bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multifunctional Conductive and Electrogenic Hydrogel Repaired Spinal Cord Injury via Immunoregulation and Enhancement of Neuronal Differentiation
Authors: Liu Mingshan , Zhang Wencan , Han Shuwei , Zhang Dapeng , Zhou Xiaolong , Guo Xianzheng , Chen Haosheng , Wang Haifeng , Jin Lin , Feng Shiqing , Wei Zhijian 
Published: February 2024

AbstractSpinal cord injury (SCI) is a refractory neurological disorder. Due to the complex pathological processes, especially the secondary inflammatory cascade and the lack of intrinsic regenerative capacity, it is difficult to recover neurological function after SCI. Meanwhile, simulating the conductive microenvironment of the spinal cord reconstructs electrical neural signal transmission interrupted by SCI and facilitates neural repair. Therefore, a double‐crosslinked conductive hydrogel (BP@Hydrogel) containing black phosphorus nanoplates (BP) is synthesized. When placed in a rotating magnetic field (RMF), the BP@Hydrogel can generate stable electrical signals and exhibit electrogenic characteristic. In vitro, the BP@Hydrogel shows satisfactory biocompatibility and can alleviate the activation of microglia. When placed in the RMF, it enhances the anti‐inflammatory effects. Meanwhile, wireless electrical stimulation promotes the differentiation of neural stem cells (NSCs) into neurons, which is associated with the activation of the PI3K/AKT pathway. In vivo, the BP@Hydrogel is injectable and can elicit behavioral and electrophysiological recovery in complete transected SCI mice by alleviating the inflammation and facilitating endogenous NSCs to form functional neurons and synapses under the RMF. The present research develops a multifunctional conductive and electrogenic hydrogel for SCI repair by targeting multiple mechanisms including immunoregulation and enhancement of neuronal differentiation.
From Two‐Step Excitation to Persistent Luminescence: Revisiting ZnGa2O4:Cr3+ Phosphor Through Upconversion Charging Approach
Authors: Liu Xueqing , Chen Lu , Huo Xiaowen , Liu Feng , Liao Chuan , Zhang Liangliang , Zhang Jiahua , Zhang Shaoan , Li Yang , Wang Xiao‐jun , Liu Yichun 
Published: February 2024

AbstractThe ZnGa2O4:Cr3+ phosphor has emerged as a significant luminescent material due to its long‐lasting afterglow and near‐infrared emission, making it suitable for applications in bioimaging and night‐vision detection. However, the limited availability of excitation light sources poses a challenge for charging the phosphor. In this study, the charging capabilities of ZnGa2O4:Cr3+ using visible lasers and a white flashlight as excitation sources are explored. By absorbing two excitation photons, the high‐lying delocalized state of Cr3+ can be excited through a two‐step process, resulting in the filling of persistent luminescence traps and producing a long‐lasting emission peaking at 696 nm. The application of the white flashlight revealed a nonlinear excitation threshold for charging at 1.5 mW cm−2. The findings also uncovered that the excitation mechanism involves excited‐state absorption and energy‐transfer upconversion. Moreover, taking advantage of the unique excitability of the near‐infrared persistent phosphor, the potential for charging persistent luminescent probes in vivo using chicken breast tissue as a representative model is showcased. The present upconversion charging approach may offer promising possibilities and introduce a novel excitation technique for ZnGa2O4:Cr3+ persistent phosphor.
Robust Free Excitons in CH3NH3PbI3Halide Perovskite Revealed by Four‐Wave Mixing
Authors: Solovev Ivan A. , Nazarov Roman S. , Murzin Aleksei O. , Selivanov Nikita I. , Gets Dmitry S. , Makarov Sergey V. , Stoumpos Constantinos C. , Kapitonov Yury V. 
Published: February 2024

AbstractDirect bandgap semiconductors possess a unique attribute: the presence of a free exciton state with a high total oscillator strength. This property makes them highly promising for applications in ultrafast optical signal processing and optical computing. One such protocol for optical computing is based on four‐wave mixing (FWM). In this study, the nonlinear optical effect in polycrystalline thin films of halide perovskite MAPbI3(MA+ = CH3NH) at low temperatures is demonstrated. Through analyzing the spectroscopy of the FWM signal, studying the photoluminescence excitation spectra, and comparing the findings with results from MAPbI3single crystals, it has been discovered that the strongest nonlinear response is observed at the free exciton resonance and in the region of shallow defect states. Surprisingly, the presence of FWM in cross‐linear excitation geometry has been observed, indicating potential involvement of other nonlinear or many‐body effects. The observations of FWM with free excitons even in highly defective MAPbI3thin films demonstrate the robustness of the exciton resonance and highlight the practical prospects for utilizing this material in optical computing.
High‐Humidity‐Tolerant Chloride Solid‐State Electrolyte for All‐Solid‐State Lithium Batteries
Authors: Wang Kai , Gu Zhenqi , Liu Haoxuan , Hu Lv , Wu Ying , Xu Jie , Ma Cheng 
Published: February 2024

AbstractHalide solid‐state electrolytes (SSEs) hold promise for the commercialization of all‐solid‐state lithium batteries (ASSLBs); however, the currently cost‐effective zirconium‐based chloride SSEs suffer from hygroscopic irreversibility, low ionic conductivity, and inadequate thermal stability. Herein, a novel indium‐doped zirconium‐based chloride is fabricated to satisfy the abovementioned requirements, achieving outstanding‐performance ASSLBs at room temperature. Compared to the conventional Li2ZrCl6 and Li3InCl6 SSEs, the hc‐Li2+xZr1‐xInxCl6 (0.3 ≤ x ≤ 1) possesses higher ionic conductivity (up to 1.4 mS cm−1), and thermal stability (350 °C). At the same time, the hc‐Li2.8Zr0.2In0.8Cl6 also shows obvious hygroscopic reversibility, where its recovery rate of the ionic conductivity is up to 82.5% after 24‐h exposure in the 5% relative humidity followed by heat treatment. Theoretical calculation and experimental results reveal that those advantages are derived from the lattice expansion and the formation of Li3InCl6 ·2H2O hydrates, which can effectively reduce the migration energy barrier of Li ions and offer reversible hydration/dehydration pathway. Finally, an ASSLB, assembled with reheated‐Li2.8Zr0.2In0.8Cl6 after humidity exposure, single‐crystal LiNi0.8Mn0.1Co0.1O2 and Li‐In alloy, exhibits capacity retention of 71% after 500 cycles under 1 C at 25 °C. This novel high‐humidity‐tolerant chloride electrolyte is expected to greatly carry forward the ASSLBs industrialization.
Targeting Grancalcin Accelerates Wound Healing by Improving Angiogenesis in Diabetes
Authors: Xiang Peng , Jiang Meng , Chen Xin , Chen Linyun , Cheng Yalun , Luo Xianghang , Zhou Haiyan , Zheng Yongjun 
Published: February 2024

AbstractChronic diabetic wounds are a serious complication of diabetes and often result in limb amputations and confer high mortality rates. The proinflammatory secretome in the wound perpetuates defective neovascularization and contributes to dysregulated tissue repair. This study aims to design a gelatin methacrylamide (GelMA) hydrogel to sustained the release of grancalcin‐neutralizing antibody (GCA‐NAb) and evaluate it as a potential scaffold to promote diabetic wound healing. Results show that the expression of grancalcin(GCA), a protein secreted by bone marrow‐derived immune cells, is elevated in the wound sites of individuals and animals with diabetic ulcers. Genetic inhibition of grancalcin expression accelerates vascularization and healing in an animal model. Mechanistic studies show that grancalcin binds to transient receptor potential melastatin 8(TRPM8) and partially inactivates its downstream signaling pathways, thereby impairing angiogenesis in vitro and ex vivo. Systemic or topical administration of a GCA‐NAb accelerate wound repair in mice with diabetes. The data suggest that GCA is a potential therapeutic target for the treatment of diabetic ulcers.
Growth‐Coupled Evolutionary Pressure Improving Epimerases for D‐Allulose Biosynthesis Using a Biosensor‐Assisted In Vivo Selection Platform
Authors: Li Chao , Gao Xin , Li Huimin , Wang Tong , Lu Fuping , Qin Hui‐Min 
Published: February 2024

AbstractFast screening strategies that enable high‐throughput evaluation and identification of desired variants from diversified enzyme libraries are crucial to tailoring biocatalysts for the synthesis of D‐allulose, which is currently limited by the poor catalytic performance of ketose 3‐epimerases (KEases). Here, the study designs a minimally equipment‐dependent, high‐throughput, and growth‐coupled in vivo screening platform founded on a redesigned D‐allulose‐dependent biosensor system. The genetic elements modulating regulator PsiR expression levels undergo systematic optimization to improve the growth‐responsive dynamic range of the biosensor, which presents ≈30‐fold facilitated growth optical density with a high signal‐to‐noise ratio (1.52 to 0.05) toward D‐allulose concentrations from 0 to 100 mm. Structural analysis and evolutionary conservation analysis of Agrobacterium sp. SUL3 D‐allulose 3‐epimerase (ADAE) reveal a highly conserved catalytic active site and variable hydrophobic pocket, which together regulate substrate recognition. Structure‐guided rational design and directed evolution are implemented using the growth‐coupled in vivo screening platform to reprogram ADAE, in which a mutant M42 (P38N/V102A/Y201L/S207N/I251R) is identified with a 6.28‐fold enhancement of catalytic activity and significantly improved thermostability with a 2.5‐fold increase of the half‐life at 60 °C. The research demonstrates that biosensor‐assisted growth‐coupled evolutionary pressure combined with structure‐guided rational design provides a universal route for engineering KEases.
A Thermosensitive Bi‐Adjuvant Hydrogel Triggers Epitope Spreading to Promote the Anti‐Tumor Efficacy of Frameshift Neoantigens
Authors: Ke Yaohua , Xin Kai , Tao Yaping , Li Lin , Chen Aoxing , Shao Jingyi , Zhu Junmeng , Zhang Dinghu , Cen Lanqi , Chu Yanhong , Yu Lixia , Liu Baorui , Liu Qin 
Published: February 2024

AbstractTumor‐specific frameshift mutations encoding peptides (FSPs) are highly immunogenic neoantigens for personalized cancer immunotherapy, while their clinical efficacy is limited by immunosuppressive tumor microenvironment (TME) and self‐tolerance. Here, a thermosensitive hydrogel (FSP‐RZ‐BPH) delivering dual adjuvants R848 (TLR7/8 agonist) + Zn2+ (cGAS‐STING agonist) is designed to promote the efficacy of FSPs on murine forestomach cancer (MFC). After peritumoral injection, FSP‐RZ‐BPH behaves as pH‐responsive sustained drug release at sites near the tumor to effectively transform the immunosuppressive TME into an inflammatory type. FSP‐RZ‐BPH orchestrates innate and adaptive immunity to activate dendritic cells in tumor‐draining lymph nodes and increase the number of FSPs‐reactive effector memory T cells (TEM) in tumor by 2.9 folds. More importantly, these TEM also exhibit memory responses to nonvaccinated neoantigens on MFC. This epitope spreading effect contributes to reduce self‐tolerance to maintain long‐lasting anti‐tumor immunity. In MFC suppressive model, FSP‐RZ‐BPH achieves 84.8% tumor inhibition rate and prolongs the survival of tumor‐bearing mice with 57.1% complete response rate. As a preventive tumor vaccine, FSP‐RZ‐BPH can also significantly delay tumor growth. Overall, the work identifies frameshift MFC neoantigens for the first time and demonstrates the thermosensitive bi‐adjuvant hydrogel as an effective strategy to boost bystander anti‐tumor responses of frameshift neoantigens.
Synthesis and Structural Analysis of High‐Silica ERI Zeolite with Spatially‐Biased Al Distribution as a Promising NH3‐SCR Catalyst
Authors: Zhu Jie , Muraoka Koki , Ohnishi Takeshi , Yanaba Yutaka , Ogura Masaru , Nakayama Akira , Wakihara Toru , Liu Zhendong , Okubo Tatsuya 
Published: February 2024

AbstractErionite (ERI) zeolite has recently attracted considerable attention for its application prospect in the selective catalytic reduction of NOx with NH3 (NH3‐SCR), provided that the high‐silica (Si/Al > 5.5) analog with improved hydrothermal stability can be facilely synthesized. In this work, ERI zeolites with different Si/Al ratios (4.6, 6.4, and 9.1) are synthesized through an ultrafast route, and in particular, a high‐silica ERI zeolite with a Si/Al ratio of 9.1 is obtained by using faujasite (FAU) as a starting material. The solid‐state 29Si MAS NMR spectroscopic study in combination with a computational simulation allows for figuring out the atomic configurations of the Al species in the three ERI zeolites. It is revealed that the ERI zeolite with the highest Si/Al ratio (ERI‐9.1, where the number indicates the Si/Al ratio) exhibits a biased Al occupancy at T1 site, which is possibly due to the presence of a higher fraction of the residual potassium cations in the can cages. In contrast, the Al siting in ERI‐4.6 and ERI‐6.4 proves to be relatively random.
Spatiotemporal Controllable Sono‐Nanovaccines Driven by Free‐Field Based Whole‐Body Ultrasound for Personalized Cancer Therapy
Authors: Wang Yang , Li Guangzhe , Su Jianlong , Liu Yiming , Zhang Xiaomai , Zhang Guanyi , Wu Zhihao , Li Jinrong , Zhang Yuxuan , Wang Xu , Yang Zejia , Wang Ruimin , Wang Chengdong , Wang Liu , Sun Fangfang , Zhao Weijie , Wang Xuejian , Peng Xiaojun , Shao Kun 
Published: February 2024

AbstractTherapeutic cancer vaccines fail to produce satisfactory outcomes against solid tumors since vaccine‐induced anti‐tumor immunity is significantly hampered by immunosuppression. Generating an in situ cancer vaccine targeting immunological cold tumor microenvironment (TME) appears attractive. Here, a type of free‐field based whole‐body ultrasound (US)‐driven nanovaccines are constructed, named G5‐CHC‐R, by conjugating the sonosensitizer, Chenghai chlorin (CHC) and the immunomodulator, resiquimod (R848) on top of a super small‐sized dendrimeric nanoscaffold. Once entering tumors, R848 can be cleaved from a hypoxia‐sensitive linker, thus modifying the TME via converting macrophage phenotypes. The animals bearing orthotopic pancreatic cancer with intestinal metastasis and breast cancer with lung metastasis are treated with G5‐CHC‐R under a free‐field based whole‐body US system. Benefit from the deep penetration capacity and highly spatiotemporal selectiveness, G5‐CHC‐R triggered by US represented a superior alternative for noninvasive irradiation of deep‐seated tumors and magnification of local immune responses via driving mass release of tumor antigens and “cold‐warm‐hot” three‐state transformation of TME. In addition to irradiating primary tumors, a robust adaptive anti‐tumor immunity is potentiated, leading to successful induction of systemic tumor suppression. The sono‐nanovaccines with good biocompatibility posed wide applicability to a broad spectrum of tumors, revealing immeasurable potential for translational research in oncology.
Mitochondrial‐Targeted CS@KET/P780 Nanoplatform for Site‐Specific Delivery and High‐Efficiency Cancer Immunotherapy in Hepatocellular Carcinoma
Authors: Liu Shanshan , Tian Hailong , Ming Hui , Zhang Tingting , Gao Yajie , Liu Ruolan , Chen Lihua , Yang Chen , Nice Edouard C. , Huang Canhua , Bao Jinku , Gao Wei , Shi Zheng 
Published: February 2024

AbstractHepatocellular carcinoma (HCC) is a form of malignancy with limited curative options available. To improve therapeutic outcomes, it is imperative to develop novel, potent therapeutic modalities. Ketoconazole (KET) has shown excellent therapeutic efficacy against HCC by eliciting apoptosis. However, its limited water solubility hampers its application in clinical treatment. Herein, a mitochondria‐targeted chemo‐photodynamic nanoplatform, CS@KET/P780 NPs, is designed using a nanoprecipitation strategy by integrating a newly synthesized mitochondria‐targeted photosensitizer (P780) and chemotherapeutic agent KET coated with chondroitin sulfate (CS) to amplify HCC therapy. In this nanoplatform, CS confers tumor‐targeted and subsequently pH‐responsive drug delivery behavior by binding to glycoprotein CD44, leading to the release of P780 and KET. Mechanistically, following laser irradiation, P780 targets and destroys mitochondrial integrity, thus inducing apoptosis through the enhancement of reactive oxygen species (ROS) buildup. Meanwhile, KET‐induced apoptosis synergistically enhances the anticancer effect of P780. In addition, tumor cells undergoing apoptosis can trigger immunogenic cell death (ICD) and a longer‐term antitumor response by releasing tumor‐associated antigens (TAAs) and damage‐associated molecular patterns (DAMPs), which together contribute to improved therapeutic outcomes in HCC. Taken together, CS@KET/P780 NPs improve the bioavailability of KET and exhibit excellent therapeutic efficacy against HCC by exerting chemophototherapy and antitumor immunity.
Temperature‐Dependent Separation of CO2 from Light Hydrocarbons in a Porous Self‐Assembly of Vertexes Sharing Octahedra
Authors: Li Shun , Li Qing , Chen Ting , Ji Zhen‐Yu , Li Guo‐Ling , Wu Ming‐Yan , Meng Li‐Yi , Nan Zi‐Ang , Wang Wei , Zhuo Zhu , Fan Fengru , Huang You‐Gui 
Published: February 2024

AbstractDesign of flexible porous materials where the diffusion of guest molecules is regulated by the dynamics of contracted pore aperture is challenging. Here, a flexible porous self‐assembly consisting of 1D channels with dynamic bottleneck gates is reported. The dynamic pendant naphthimidazolylmethyl moieties at the channel necks provide kinetic gate function, that enables unusual adsorption for light hydrocarbons. The adsorption for CO2 is mainly dominated by thermodynamics with the uptakes decreasing with increasing temperature, whereas the adsorptions for larger hydrocarbons are controlled by both thermodynamics and kinetics resulting in an uptake maximum at a temperature threshold. Such an unusual adsorption enables temperature‐dependent separation of CO2 from the corresponding hydrocarbons.
High Sensitivity and Ultra‐Broad‐Range NH3 Sensor Arrays by Precise Control of Step Defects on The Surface of Cl2‐Ndi Single Crystals
Authors: Lu Bin , Stolte Matthias , Liu Dong , Zhang Xiaojing , Zhao Lihui , Tian Liehao , Frisbie C. Daniel , Würthner Frank , Tao Xutang , He Tao 
Published: February 2024

AbstractVapor sensors with both high sensitivity and broad detection range are technically challenging yet highly desirable for widespread chemical sensing applications in diverse environments. Generally, an increased surface‐to‐volume ratio can effectively enhance the sensitivity to low concentrations, but often with the trade‐off of a constrained sensing range. Here, an approach is demonstrated for NH3 sensor arrays with an unprecedentedly broad sensing range by introducing controllable steps on the surface of an n‐type single crystal. Step edges, serving as adsorption sites with electron‐deficient properties, are well‐defined, discrete, and electronically active. NH3 molecules selectively adsorb at the step edges and nearly eliminate known trap‐like character, which is demonstrated by surface potential imaging. Consequently, the strategy can significantly boost the sensitivity of two‐terminal NH3 resistance sensors on thin crystals with a few steps while simultaneously enhancing the tolerance on thick crystals with dense steps. Incorporation of these crystals into parallel sensor arrays results in ppb–to–% level detection range and a convenient linear relation between sheet conductance and semi‐log NH3 concentration, allowing for the precise localization of vapor leakage. In general, the results suggest new opportunities for defect engineering of organic semiconductor crystal surfaces for purposeful vapor or chemical sensing.
H2S‐Releasing Versatile Montmorillonite Nanoformulation Trilogically Renovates the Gut Microenvironment for Inflammatory Bowel Disease Modulation
Authors: Jin Ting , Lu Hongyang , Zhou Qiang , Chen Dongfan , Zeng Youyun , Shi Jiayi , Zhang Yanmei , Wang Xianwen , Shen Xinkun , Cai Xiaojun 
Published: February 2024

AbstractAbnormal activation of the intestinal mucosal immune system, resulting from damage to the intestinal mucosal barrier and extensive invasion by pathogens, contributes to the pathogenesis of inflammatory bowel disease (IBD). Current first‐line treatments for IBD have limited efficacy and significant side effects. An innovative H2S‐releasing montmorillonite nanoformulation (DPs@MMT) capable of remodeling intestinal mucosal immune homeostasis, repairing the mucosal barrier, and modulating gut microbiota is developed by electrostatically adsorbing diallyl trisulfide‐loaded peptide dendrimer nanogels (DATS@PDNs, abbreviated as DPs) onto the montmorillonite (MMT) surface. Upon rectal administration, DPs@MMT specifically binds to and covers the damaged mucosa, promoting the accumulation and subsequent internalization of DPs by activated immune cells in the IBD site. DPs release H2S intracellularly in response to glutathione, initiating multiple therapeutic effects. In vitro and in vivo studies have shown that DPs@MMT effectively alleviates colitis by eliminating reactive oxygen species (ROS), inhibiting inflammation, repairing the mucosal barrier, and eradicating pathogens. RNA sequencing revealed that DPs@MMT exerts significant immunoregulatory and mucosal barrier repair effects, by activating pathways such as Nrf2/HO‐1, PI3K‐AKT, and RAS/MAPK/AP‐1, and inhibiting the p38/ERK MAPK, p65 NF‐κB, and JAK‐STAT3 pathways, as well as glycolysis. 16S rRNA sequencing demonstrated that DPs@MMT remodels the gut microbiota by eliminating pathogens and increasing probiotics. This study develops a promising nanoformulation for IBD management.
BCG as an Innovative Option for HCC Treatment: Repurposing and Mechanistic Insights
Authors: Vaziri Farzam , Setayesh Tahereh , Hu Ying , Ravindran Resmi , Wei Dongguang , Wan Yu‐Jui Yvonne 
Published: February 2024

AbstractThis study investigates Bacillus Calmette‐Guérin (BCG) as a potential treatment for hepatocellular carcinoma (HCC), a condition often associated with unfavorable treatment outcomes. Exploiting BCG's recognized immune‐boosting properties, preclinical trials are conducted using HCC mice, with a single subcutaneous dose of BCG administered post‐tumor formation. Results indicate that BCG treatment effectively diminishes tumor burden and extends survival in both male and female HCC mice. Positive influences on hepatic fibrosis and metabolism are observed, leading to a reduction in lipid levels. Spatial analysis underscores BCG's tumor‐specific effects, inducing the enrichment of metabolic pathways and inhibiting various cancer‐related pathways. Furthermore, BCG promotes immune cell infiltration, including CD4+, CD8+ T cells, and M1 macrophages, in both v‐akt murine thymoma viral oncogene homolog 1(AKT)/neutoblastoma RAS viral oncogene homolog (RAS) and β‐catenin positive HCC models. Interestingly, blocking T cells, trained immunity, and Interferon‐γ (IFN‐γ) function reverses BCG's anti‐HCC effects. In conclusion, BCG emerges as a promising treatment option for HCC, characterized by a favorable safety profile and efficacy in inhibiting fibrosis, improving metabolism, and engaging both trained immunity and T cells in therapeutic mechanisms.