bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of bile acid TLC-S vitality of pantacric acinary cells and level of mitochondrial membrane potential
Authors:   Ivan Franko National University of Lviv, Manko B.V. 
Published: October 2023

To investigate the mechanism of action of bile acids, TLC-S, the viability of pancreatic acinar cells, and the level of mitochondrial membrane potential were studied using various oxidation substrates. The experiments were conducted on male Wistar rats weighing 250–300 g. A suspension of isolated pancreatic acinar cells was obtained using collagenase (type IV, 0.2 mg/ml). The trypan blue exclusion test assessed cell viability after isolation and the amount was >90 %. The survival of acinar cells after prolonged incubation in different environments was determined by fluorescent dyes propidium iodide (0.5 mg/ml) and Hoechst 33258 (5 mg/ml) dyes. Mitochondrial membrane potential was recorded by adding the dye TMRM (50 nM). Cells were photographed using an inverted microscope Olympus IX73 with a digital camera DP-74. Cell counting and fluorescence intensity of TMRM were analyzed using ImageJ software. It was found that the presence of TLC-S in the medium at a concentration of 0.5 mmol/L, when used as an oxidation substrate for glucose (10 mmol/L), pyruvate (2 mmol/L), or alanine (2 mmol/L), did not significantly affect the proportion of live cells after 2 and 4 hours of incubation. However, when TLC-S was added to the medium at a concentration of 2 mmol/L, the proportion of live cells significantly decreased. This decrease was least pronounced during pyruvate oxidation. Under the action of TLC-S (after 25 min) in the presence of glucose alone, the mitochondrial membrane potential decreased by 9.8 % (P<0.05, n=3), in the presence of alanine (in the presence of glucose) – by 23.5 % (P<0.05, n=4). When pyruvate was added to the medium (in the presence of glucose), the mitochondrial membrane potential likely did not change significantly. These results suggest the presence of several different mechanisms of TLC-S’s negative effects on pancreatic acinar cells: at low concentrations (or at early stages), it disrupts alanine transamination reactions, leading to the disruption of mitochondrial membrane potential generation, while at high concentrations, it affects the integrity of plasma and/or intracellular membranes.
TRATAMENTO CONSERVADOR NA UNIDADE BÁSICA DE SAÚDE
Authors: De Lima Rafael Abrantes , Silva de Souza Bianca Beatriz , Andrade da Rocha Izabella , Gerbassi Costa Aguiar Beatriz 
Published: October 2023

Objetivo: Ampliar o debate sobre a atuação do Enfermeiro Nefrologista nos ambulatórios de tratamento conservador em nefrologia, tomando como foco em educação em saúde, mitigando os impactos na saúde doa pacientes renais crônicos. Métodos: Estudo teórico sobre o desenvolvimento operacional do enfermeiro nefrologista no tratamento conservador em nefrologia. Desenvolvimento: Além disso, o enfermeiro nefrologista desempenha um papel crucial na prevenção de complicações, na identificação precoce de alterações no estado de saúde e na tomada de decisões em conjunto com os demais profissionais de saúde. Considerações Finais: O Enfermeiro Nefrologista desempenha um papel central na orientação, educação e suporte aos pacientes, colaborando para a prevenção de complicações e o alcance de uma melhor qualidade de vida. Portanto, é fundamental reconhecer e fortalecer a importância desse profissional na assistência aos pacientes renais crônicos.
Limits of the U.S. Power in the Current Global World
Authors: KARAZANISHVILI Tamar 
Published: October 2023

In a little over a decade, geopolitics has moved to the “Multipolar Era”, and has entered the twenty-first century, which is likely to see the distribution of economic and political power and eventually military capabilities spread across a broader range of national “actors.” U.S. global role has consequently started decreasing in various areas, and America’s capacity to influence choices made by Russia, China, India, Japan and others countries of different regions has been reduced. The rising powers are charting their own courses. The article deeply analyses the limits of U.S. power and its role in global world, starting with the brief historical review and developing into the discussion of the current period. It gives information regarding the U.S. relations and importance with different countries, while emphasizing the global view of the U.S. role in the world. The paper discusses the effectiveness of today’s America and shows reasons for prompting declination of the U.S. power in the current world affairs, while at the same time, reveals another side of the U.S. power still being a leading country in the world, analyzed well in conclusion.
European Integration as one of the Main Foreign Policy and National Security Priorities of Georgia
Authors: CHITADZE Nika 
Published: October 2023

The paper explores the basic socio-economic and political processes, which were going on in Georgia after the restoration of national independence in the beginning of the 90-th of the XX Century and which were related to the economic difficulties and conflicts in the two separatist regions of Georgia; also, the reasons, which determined the desire of Georgia, to develop cooperation with western democratic institutions – first of all with the European Union. The second part of the research is concentrated on the modern period, particularly, on the basic issues, related to the geopolitical importance of Georgia in the region, taking into consideration new geopolitical realities - main foreign policy and national security priorities of the country, reasons for the European Integration of Georgia, basic priorities of the EU Eastern Partnership Program and cooperation between EU and Georgia in different fields.
Geopolitical Issues in the Current Crisis Between Ukraine and Russia
Authors: GIRGIN Dogan 
Published: October 2023

This article concentrates on the new geopolitical role and place of Ukraine, taking into account consequences of the military conflict with Russia. After the Soviet Union collapsed, a huge transformation occurred because of the critical role and geopolitical importance of Ukraine for both Russia and the EU. The former considers it strategically important not only as a littoral state at the Black Sea that borders on several EU member states but also a transit country for Russian gas. In turn, the European Union occupies a cautious stance towards both Ukraine and Russia weighting positive and negative developments that its decisions may cause. Depending on the results of the future presidential elections, the United States might continue to pursue aims that would further irritate Russia or they will prefer to avoid actions that would risk harming Moscow’s ties with Washington.
The Role and Place of Georgia in the Globalized World
Authors: MKALAVISHVILI Tamar 
Published: October 2023

The article focuses on explaining the importance of the role of Georgia in the globalization processes. Main positive aspects are indicated with the Scientificcitations. Foreign policy has become one of the main important challenges of the new government of Georgia so article will provide you with the information about the relationship between Georgia and EU, NATO, and USA. Foreign Trade of Georgia is one of the most important factors,which determines the country’s active involvement in the Globalization processes and my article aims to provide you with important information concerning the main crucial processes in this direction.
Psychosocial Predictors of Drug Involvement Among Secondary School Students
Authors: ASAGBA Rachel B. , CHOVWEN Catherine O. , ALARAPE Aderemi l. , AGBEROTIMI Samson F. 
Published: October 2023

Predictors of drug involvement among secondary school students in Ibadan were examined in this study. Two hundred and four (204) students comprising 113 males and 91 females, whose age ranged between 13 and 25 years (M = 16.32, S.D. = 1.81) participatedin the study. Data collected with a structured questionnaire were subjected to independent sample t-test, and regression analysis. Psychosocial factors (life purpose, need for achievement and self-esteem) significantly predicted drug abuse [F (6, 113) = 7.75; p < .01, R2 = .29]. Participants with low score on life purpose reported higher substanceabuse than those with high score on life purpose, t (161) = 2.62, p <.05 ; those with low score on the need for achievement reported higher substance abuse than those with high score on the need for achievement, t (162) = 4.09, p < .01 ; those with low score on the need for achievement reported higher substance abuse than those with high scoreon the need for achievement t (162) = 2.62, p <.05; female participants reported higher substance abuse than male counterparts, t (176) = - 4.05, p <.01). In conclusion, it is expedient that efforts be intensified on actions that will reawaken the strive for excellence and sense of self worth through engagement in self development activities, thereby minimizingthe prevalence of substance abuse among Nigerian youths.
THE AMERICAN WOMAN IDENTITY AS REFLECTED IN EAT PRAY LOVE NOVEL IN HER PURSUIT OF HAPPINESS
Authors: Ardian Edi , Amri Samsul , Putra Edi Susrianto Indra 
Published: October 2023

American culture background influences the content of literary work in this novel. The purpose of the research is to know an American woman identity as reflected in Eat Pray Love (EPL) novel in her pursuit of happiness. For that purpose, a thematic and descriptive, analysis is conducted to answer two research problems: 1) what constraint of woman to face in her attempts to achieve happiness; and 2) How the novel reflects of the American woman identity in her pursuit of happiness? The analysis finds out that woman faces the problem based on her culture. She is unhappy in her marriage, but the she deal with her problem in American culture identity, she attempts individual redefinition of the self by undergoing material and spiritual search and fulfillment, and finally managed to achieve her true happiness. The ego wins over the superego, and a new identity comes out of an internal conflict and an intense spiritual process. American individualism allows this. American individualism gives greater priority to individuals.
Pembuatan Cake Tepung Ubi Jalar Ungu Dengan Penambahan Pure Buah Nangka Terhadap Nilai Antioksidan, Karakteristik Kimia dan Organoleptik
Authors: Mita Elly , Rochmah Alfi Nur 
Published: October 2023

Cake merupakan makanan ringan dengan rasa manis yang menggunakan adonan dari tiga bahan utama, yaitu tepung terigu, telur dan gula. Penambahan buah nangka murni memiliki aroma, rasa yang khas dan potensi antioksidan maka dapat digunakan sebagai bahan komposisi dalam pembuatan kue tepung ubi jalar ungu. Penelitian ini bertujuan untuk mengetahui nilai antioksidan, karakteristik kimia dan organoleptik kue tepung ubi jalar ungu. Sampel yang digunakan dalam pembuatan kue tepung ubi jalar ungu dengan penambahan buah nangka murni yaitu tanpa penambahan buah nangka murni (kontrol), penambahan 20% buah nangka murni dari total penggunaan tepung, penambahan 30% buah nangka murni dari total penggunaan tepung dan penambahan 40% buah nangka murni dari total penggunaan tepung. Berdasarkan hasil organoleptik, uji SPSS versi 23 dan uji De Garmo sampel terpilih yaitu formulasi penambahan 30% buah nangka murni dari total penggunaan tepung, kemudian dilakukan analisis kimia meliputi kadar udara, kadar abu, kadar protein, kadar lemak, kadar karbohidrat dan nilai antioksidan. Hasil analisis sampel kimia terpilih menunjukkan nilai kadar udara sebesar 32,76%, kadar abu sebesar 1,33%, kadar protein sebesar 4,76%, kadar lemak sebesar 18,45%, kadar karbohidrat sebesar 42,7% dan nilai antioksidan sebesar 21,6%.
Kata kunci : Kue, Tepung Ubi Jalar Ungu, Buah Nangka Murni
Classifying BISINDO Alphabet using TensorFlow Object Detection API
Authors: Hayati Lilis Nur , Handayani Anik Nur , Irianto Wahyu Sakti Gunawan , Asmara Rosa Andrie , Indra Dolly , Fahmi Muhammad 
Published: October 2023

Indonesian Sign Language (BISINDO) is one of the sign languages used in Indonesia. The process of classifying BISINDO can be done by utilizing advances in computer technology such as deep learning. The use of the BISINDO letter classification system with the application of the MobileNet V2 FPNLite SSD model using the TensorFlow object detection API. The purpose of this study is to classify BISINDO letters A-Z and measure the accuracy, precision, recall, and cross-validation performance of the model. The dataset used was 4054 images with a size of consisting of 26 letter classes, which were taken by researchers by applying several research scenarios and limitations. The steps carried out are: dividing the ratio of the simulation dataset 80:20, and applying cross-validation (k-fold = 5). In this study, a real time testing using 2 scenarios was conducted, namely testing with bright light conditions of 500 lux and dim light of 50 lux with an average processing time of 30 frames per second (fps). With a simulation data set ratio of 80:20, 5 iterations were performed, the first iteration yielded a precision result of 0.758 and a recall result of 0.790, and the second iteration yielded a precision result of 0.635 and a recall result of 0.77, then obtained an accuracy score of 0.712, the third iteration provides a recall score of 0.746, the fourth iteration obtains a precision score of 0.713 and a recall score of 0.751, the fifth iteration gives a precision score of 0.742 for a fit score case and the recall score is 0.773. So, the overall average precision score is 0.712 and the overall average recall score is 0.747, indicating that the model built performs very well.
Análisis del alcance de la conciliación extrajudicial frente a las cargas procesales en familia
Authors: Quinde Estrella Klever Agustín , Plasencia Sánchez Liliana Gisele , Calle Masache Nube Catalina 
Published: October 2023

Introducción: La conciliación extrajudicial juega un rol importante en la administración de justicia ecuatoriana, ya que busca ser un complemento a la justicia ordinaria y reducir el congestionamiento de procesos en familia que se tramitan en vía judicial. Objetivo: Determinar si la conciliación extrajudicial vulnera el principio de celeridad y si es efectiva en el amparo a la tutela judicial efectiva por medio de los Centros de Mediación del Consejo de la Judicatura es el objetivo del presente trabajo; Metodología: Para ello se opta por una investigación no experimental de forma descriptiva, investigativa y dogmática, así como el uso de fuentes bibliográficas. Resultados y Conclusión: Este análisis reconoce las circunstancias que afectan la aplicación de la conciliación extrajudicial en los Centros de Mediación debidamente acreditados. Área de estudio general: Derecho. Área de estudio específico: Familia.
Tingkat Kecemasan Atlet Karate saat Bertanding Di Perguruan Lemkari Kota Bengkulu
Authors: Huzaimah  , Nopiyanto Yahya Eko , Rizky Oddie Barnanda 
Published: October 2023

ABSTRAK
Penelitian ini bertujuan untuk mengetahui tingkat kecemasan atlet karate di perguruan LEMKARI Kota Bengkulu. Jenis penelitian yang digunakan yaitu penelitian kualitataif. Metode penelitiannya yaitu menggunakan metode deskriptif kualitatif. Subjek penelitiannya adalah atlet karate LEMKARI Kota Bengkulu yang berjumlah 21 atlet dengan menggunakan teknik purposive sampling. Teknik pengumpulan data menggunakan observasi, angket dan dokumentasi. Teknik analisis data yang digunakan yaitu analisis deskriptif dengan teknik persentase. Sedangkan pada hasil kuesioner tingkat kecemasan atlet karate di perguruan LEMKARI berada pada kategori “sedang” yaitu dengan persentase 66.67%. dari penelitian ini didapat bahwa hasil penelitian tingkat kecemasan atlet banyak terletak pada indikator kognitif dengan persentase 62% dengan jumlah 13 atlet.

Kata kunci: Atlet, Karate, Kecemasan, Strategi
Identification of optimal feature genes in patients with thyroid associated ophthalmopathy and their relationship with immune infiltration: a bioinformatics analysis
Authors: Xiong Chao , Wang Yaohua , Li Yue , Yu Jinhai , Wu Sha , Wu Lili , Zhang Boyuan , Chen Yunxiu , Gan Puying , Liao Hongfei 
Published: October 2023

BackgroundThyroid associated ophthalmopathy (TAO) is an organ-specific autoimmune disease that has a significant impact on individuals and society. The etiology of TAO is complicated and poorly understood. Thus, the goal of this study was to use bioinformatics to look into the pathogenesis of TAO and to identify the optimum feature genes (OFGs) and immune infiltration patterns of TAO.MethodsFirstly, the GSE58331 microarray data set was utilized to find 366 differentially expressed genes (DEGs). To find important modular genes, the dataset was evaluated using weighted gene coexpression network analysis (WGCNA). Then, the overlap genes of major module genes and DEGs were further assessed by applying three machine learning techniques to find the OFGs. The CIBERSORT approach was utilized to examine immune cell infiltration in normal and TAO samples, as well as the link between optimum characteristic genes and immune cells. Finally, the related pathways of the OFGs were predicted using single gene set enrichment analysis (ssGSEA).ResultsKLB, TBC1D2B, LINC01140, SGCG, TMEM37, and LINC01697 were the six best feature genes that were employed to create a nomogram with high predictive performance. The immune cell infiltration investigation revealed that the development of TAO may include memory B cells, T cell follicular helper cells, resting NK cells, macrophages of type M0, macrophages of type M1, resting dendritic cells, active mast cells, and neutrophils. In addition, ssGSEA results found that these characteristic genes were closely associated with lipid metabolism pathways.ConclusionIn this research, we found that KLB, TBC1D2B, LINC01140, SGCG, TMEM37, and LINC01697 are intimately associated with the development and progression of TAO, as well as with lipid metabolism pathways.
Research on carbon emission prediction and economic policy based on TCN-LSTM combined with attention mechanism
Authors: Wei Xiaoyan , Xu Ying 
Published: October 2023

IntroductionIn the face of increasingly severe global climate change and environmental challenges, reducing carbon emissions has become a key global priority. Deep learning, as a powerful artificial intelligence technology, has demonstrated significant capabilities in time series analysis and pattern recognition, opening up new avenues for carbon emission prediction and policy development.MethodsIn this study, we carefully collected and pre-processed four datasets to ensure the reliability and consistency of the data. Our proposed TCN-LSTM combination architecture effectively leverages the parallel computing capabilities of TCN and the memory capacity of LSTM, more efficiently capturing long-term dependencies in time series data. Furthermore, the introduction of an attention mechanism allows us to weigh important factors in historical data, thereby improving the accuracy and robustness of predictions. ResultsOur research findings provide novel insights and methods for advancing carbon emission prediction. Additionally, our discoveries offer valuable references for decision-makers and government agencies in formulating scientifically effective carbon reduction policies. As the urgency of addressing climate change continues to grow, the progress made in this paper can contribute to a more sustainable and environmentally conscious future. DiscussionIn this paper, we emphasize the potential of deep learning techniques in carbon emission prediction and demonstrate the effectiveness of the TCN-LSTM combination architecture. The significant contribution of this research lies in providing a new approach to address the carbon emission prediction problem in time series data. Moreover, our study underscores the importance of data reliability and consistency for the successful application of models. We encourage further research and application of this method to facilitate the achievement of global carbon reduction goals.
Identification of MARK2, CCDC71, GATA2, and KLRC3 as candidate diagnostic genes and potential therapeutic targets for repeated implantation failure with antiphospholipid syndrome by integrated bioinformatics analysis and machine learning
Authors: Zhang Manli , Ge Ting , Zhang Yunian , La Xiaolin 
Published: October 2023

BackgroundAntiphospholipid syndrome (APS) is a group of clinical syndromes of thrombosis or adverse pregnancy outcomes caused by antiphospholipid antibodies, which increase the incidence of in vitro fertilization failure in patients with infertility. However, the common mechanism of repeated implantation failure (RIF) with APS is unclear. This study aimed to search for potential diagnostic genes and potential therapeutic targets for RIF with APS.MethodsTo obtain differentially expressed genes (DEGs), we downloaded the APS and RIF datasets separately from the public Gene Expression Omnibus database and performed differential expression analysis. We then identified the common DEGs of APS and RIF. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed, and we then generated protein-protein interaction. Furthermore, immune infiltration was investigated by using the CIBERSORT algorithm on the APS and RIF datasets. LASSO regression analysis was used to screen for candidate diagnostic genes. To evaluate the diagnostic value, we developed a nomogram and validated it with receiver operating characteristic curves, then analyzed these genes in the Comparative Toxicogenomics Database. Finally, the Drug Gene Interaction Database was searched for potential therapeutic drugs, and the interactions between drugs, genes, and immune cells were depicted with a Sankey diagram.ResultsThere were 11 common DEGs identified: four downregulated and seven upregulated. The common DEG analysis suggested that an imbalance of immune system-related cells and molecules may be a common feature in the pathophysiology of APS and RIF. Following validation, MARK2, CCDC71, GATA2, and KLRC3 were identified as candidate diagnostic genes. Finally, Acetaminophen and Fasudil were predicted as two candidate drugs.ConclusionFour immune-associated candidate diagnostic genes (MARK2, CCDC71, GATA2, and KLRC3) were identified, and a nomogram for RIF with APS diagnosis was developed. Our findings may aid in the investigation of potential biological mechanisms linking APS and RIF, as well as potential targets for diagnosis and treatment.
Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies
Authors: Wright Robin D. , Bartelli Thais F. , Baydogan Seyda , White James Robert , Kim Michael P. , Bhutani Manoop S. , McAllister Florencia 
Published: October 2023

IntroductionThe tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult to define the TM prior to surgical resection.MethodsIn this pilot feasibility study, patients underwent Endoscopic Ultrasound-Fine Needle Aspiration (EUS-FNA) biopsy of pancreatic adenocarcinoma. These samples were analyzed using 16S rRNA and internal transcribed spacer (ITS) sequencing for characterization of the tumor bacteria and fungi.ResultAfter in silico decontamination and comparison to non-matched tumor, we were able to characterize the TM in biopsies, which was comparable to the TM from surgical specimens.DiscussionEUS-FNA biopsy may represent a feasible modality to characterize the pancreatic TM prior to surgical resection with proper decontamination strategies and improvements in matched controls.