bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of short-term active video game play on community adults: under International Classification of Functioning, Disability and Health consideration
Authors: Wei-Che Tseng , Ru-Lan Hsieh 
Published: January 2024

Background
The effects of active video game play on healthy individuals remain uncertain. A person's functional health status constitutes a dynamic interaction between components identified in the International Classification of Functioning, Disability, and Health (ICF). The aim of this study was to investigate the short-term effects of active video game play on community adults using the ICF.

Methods
Sixty community adults with an average age of 59.3 years and without physical disabilities were recruited. Over 2 weeks, each adult participated in six sessions of active video game play lasting 20 minutes each. Participants were assessed before and after the intervention. Variables were collected using sources related to the ICF components, including the Hospital Anxiety and Depression Scale, Multidimensional Fatigue Inventory, Biodex Stability System, chair-rising time, Frenchay Activity Index, Rivermead Mobility Index, Chronic Pain Grade Questionnaire, Work Ability Index, and World Health Organization Quality of Life-Brief Version.

Results
Compared to baseline data, significantly reduced risk of a fall measured by Biodex Stability System and improvements in disability scores measured by the Chronic Pain Grade Questionnaire were noted. There was no significant change in the other variables measured.

Conclusion
Short-term, active video game play reduces fall risks and ameliorates disabilities in community adults.
Usefulness of lumen area parameters determined by intravascular ultrasound to predict functional significance of intermediate coronary artery stenosis
Authors: CUI Ming , ZHU Dan , GUO Li-jun , SONG Li-ying , ZHANG Yong-zhen , ZHANG Fu-chun , NIU Jie , WANG Gui-song , HAN Jiang-li , GAO Wei 
Published: January 2024

Background
Coronary artery disease is the leading cause of death in China. Percutaneous coronary intervention is a recent milestone technology for treatment coronary artery disease. However, clinical decision making for patients with intermediate coronary stenosis is still controversial. We designed this study to assess the optimal intravascular ultrasound (IVUS) criteria for predicting functional significance of intermediate coronary lesions.

Methods
We enrolled 141 patients with 165 intermediate coronary lesions located in vessels with a diameter ≥ 2.50 mm. IVUS of intermediate coronary lesions were performed before intervention. Pressure-derived fractional flow reserve (FFR) was measured at maximal hyperemia induced by adenosine infusion. An FFR <0.80 was considered as abnormal functional significance.

Results
For the overall 165 lesions, the mean FFR value was 0.84±0.09. The diameter of the stenosis by visual estimation on angiogram was (59.63±11.29)%. Minimum lumen diameter (MLD), minimum lumen area (MLA) and plaque burden (PB) were (2.00±0.36) mm, (3.88±1.34) mm2, (67.28±9.89)% respectively by IVUS measurements. An FFR <0.80 was seen in 43 lesions (30.5%). There was a moderate correlation between IVUS parameters and FFR, including MLD (r=0.372, P <0.001), MLA (r=0.442, P <0.001) and PB (r=-0.172, P <0.05). MLA was a predictor for FFR as a continuous variable independent of possible confounding variables (P <0.05), and MLA and PB, were predictors for FFR <0.80 as binary variables (P <0.05). The best cutoff value of MLA to predict FFR <0.80 was <3.15 mm2, with a 73.6% diagnostic accuracy; sensitivity 71.4%, specificity 67.0%, AUC=0.709, and P <0.001. The cutoff value of the PB to predict FFR <0.80 was 65.45%; sensitivity 82.6%, specificity 41.2%, AUC=0.644, and P <0.01. If both MLA and PB were taken into account, the negative predictive value and the positive predictive value were 88.7% and 64.8% respectively.

Conclusions
Anatomic measurements of intermediate coronary lesions obtained by IVUS showed a moderate correlation to FFR values. IVUS-derived MLA ≥3.15 mm2 may be useful to exclude FFR <0.80, but poor specificity limits its applicability for physiological assessment of lesions <3.15 mm2. MLA was one of many factors affecting coronary flow hemodynamics. Both MLA and PB should be taken into account when determining functional ischemia.
Effector cells derived from naive T cells used in tumor immunotherapy of mice bearing B16 melanoma
Authors: Wen Ming , Xu Weili , Ren Lili , Gao Fei , Cui Naipeng , Wen Junye , Li Xinjiang , Lin Lin , Ma Zhenfeng , Chen Baoping , Cai Jianhui 
Published: January 2024

Background
Adoptive cell transfer (ACT) immunotherapy has been used clinically for years to treat malignancies. Improving the killing efficiency of effector cells, such as tumor-specific cytotoxic T lymphocytes (CTLs), is an important component for enhancing the clinical response of cancer immunotherapy. Hence, we explored a novel method for preparing cancer-specific CTLs using naive T lymphocytes.

Methods
C57BL/6 mice bearing B16 melanoma tumors were pretreated with cyclophosphamide (CTX) by peritoneal injection. The immunosuppressive influence of CTX on tumor regression and the tumor microenvironment was assessed. Naive T cells and T cell pools were isolated via negative selection using immunomagnetic beads. The proliferative potential and cytokine production of different T cell subpopulations were evaluated in vitro. Tumor-specific CTLs derived from naive T cells (naive CD4+ T cells: naive CD8+ T cells=2:1) and pooled T cells were generated in vitro, respectively. B16 melanoma-bearing C57BL/6 mice were pretreated with CTX, followed by ACT immunotherapy using dendritic cell-induced CTLs. The homing abilities of the effector cells and interleukin-2 (IL-2), interferon-ã, granzyme B, and perforin mRNA levels in tumor tissues were evaluated, and the change in tumor volume was measured.

Results
Mice receiving CTX peritoneal pretreatment injections did not display tumor regression compared with control mice. However, a significant downregulation of splenic Tregs and tumor growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) serum levels was observed (P <0.05). Naive T cells showed a stronger proliferative capacity and elevated cytokine production than did pooled T cells (P <0.05). In addition, effector cells generated from naive T cells displayed more potent antitumor activity in vivo than those derived from pooled T cells (P <0.05).

Conclusion
Effector cells derived from the naive T cells possess a stronger proliferative potential, homing capacity, and enhanced cytokine production, which leads to a superior antitumor response.
Inhibition of nuclear factor-κB activity enhanced chemosensitivity to cisplatin in human lung adeno-carcinoma A549 cells under chemical hypoxia conditions
Authors: LI Fang , HUANG Li , SU Xiao-li , GU Qi-hua , HU Cheng-ping 
Published: January 2024

Background
Tumor hypoxia, one of the features of solid tumors, is associated with chemo-resistance. Recently, nuclear factor-κB (NF-κB) was found to be activated during hypoxia. However, the impact of NF-κB activation on chemo-resistance during hypoxia remains unknown.

Methods
Human lung adenocarcinoma A549 cells were transfected with NF-κB p65siRNA and treated with cobalt chloride (CoCl2) to mimic hypoxia in the presence or absence of cisplatin. NF-κB expression was measured by Western blotting, immune-fluorescence and real-time PCR. Hypoxia-inducible factor-1α (HIF-1α) and Bcl-2 expression were determined by Western blotting. Cell apoptosis and survival with half-maximum inhibitory concentration (IC50) of cisplatin were determined by Annexin V-FITC/PI and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), respectively.

Results
Exposure of A549 cells to CoCl2 increased nuclear HIF-1α protein expression, and enhanced NF-κB p65 protein nuclear accumulation (the mark of NF-κB activation) in a time and dose dependant manner. CoCl2 did not promote apoptosis in A549 cells; on the contrary, it reduced cisplatin-induced apoptosis and increased the IC50 of cisplatin. However, when we inhibited CoCl2-induced activation of NF-κB through NF-κB p65siRNA, cisplatin-induced apoptosis was increased and IC50 of cisplatin was reduced to levels similar to those in control cells. Meanwhile, CoCl2-induced Bcl-2 over-expression was down-regulated in the presence of cisplatin when NF-κB activity was inhibited.

Conclusion
Up-regulating Bcl-2 might be involved in NF-κB activation induced resistance to cisplatin in A549 cells under CoCl2-induced chemical hypoxia.
Impact of the origin of sinus node artery on recurrence after pulmonary vein isolation in patients with paroxysmal atrial fibrillation
Authors: ZHANG Zhi-jun , CHEN Ke , TANG Ri-bo , SANG Cai-hua , LOPES LAO Edmundo Patricio , YAN Qian , HE Xiao-nan , DU Xin , LONG De-yong , YU Rong-hui , DONG Jian-zeng , MA Chang-sheng 
Published: January 2024

Background
Major atrial coronary arteries, including the sinus node artery (SNA), were commonly found in the areas involved in atrial fibrillation (AF) ablation and could cause difficulties in achieving linear block at the left atrial (LA) roof. The SNA is a major atrial coronary artery of the atrial coronary circulation. This study aimed to determine impact of the origin of SNA on recurrence of AF after pulmonary vein isolation (PVI) in patients with paroxysmal AF.

Methods
Seventy-eight patients underwent coronary angiography for suspected coronary heart disease, followed by catheter ablation for paroxysmal AF. According to the origin of SNA from angiographic findings, they were divided into right SNA group (SNA originating from the right coronary artery) and left SNA group (SNA originating from the left circumflex artery). Guided by an electroanatomic mapping system, circumferential pulmonary vein ablation (CPVA) was performed in both groups and PVI was the procedural endpoint. All patients were followed up at 1, 3, 6, 9 and 12 months post-ablation. Recurrence was defined as any episode of atrial tachyarrhythmias (ATAs), including AF, atrial flutter or atrial tachycardia, that lasted longer than 30 seconds after a blanking period of 3 months.

Results
The SNA originated from the right coronary artery in 34 patients (43.6%) and the left circumflex artery in 44 patients (56.4%). Freedom from AF and antiarrhythmic drugs (AADs) at 1 year was 67.9 % (53/78) for all patients. After 1 year follow-up, 79.4% (27/34) in right SNA group and 59.1% (26/44) in left SNA group (P=0.042) were in sinus rhythm. On multivariate analysis, left atrium size (HR=1.451, 95%CI: 1.240-1.697, P <0.001) and a left SNA (HR=6.22, 95%CI: 2.01-19.25, P=0.002) were the independent predictors of AF recurrence.

Conclusions
The left SNA is more frequent in the patients with paroxysmal AF. After one year follow-up, the presence of a left SNA was identified as an independent predictor of AF recurrence after CPVA in paroxysmal AF.
Pattern of instent neointimal formation compared to native atherosclerosis in the coronary bifurcation lesions: volumetric intravascular ultrasound analysis
Authors: XU Jian-qiang , Song Young Bin , Hahn Joo-Yong , Choi Seung-Hyuk , Choi Jin-Ho , LU Cheng-zhi , Lee Sang Hoon , Hong Kyung Pyo , Park Jeung Euy , Gwon Hyeon-Cheol 
Published: January 2024

Background
No clinical study has systematically analyzed and compared circumferential neointimal and plaque distribution of stent neointimal proliferation and in native atherosclerotic plaques. This study aimed to investigate and compare the pattern of instent neointimal formation and native atherosclerosis in the coronary bifurcation lesions by volumetric analysis using systematic intravascular ultrasound (IVUS).

Methods
We examined bifurcation lesions in native coronary artery (plaque group, n=102) and stented bifurcations at 9-month follow-up (neointima group, n=51) using volumetric IVUS analysis of both the main vessel (MV) and side branch (SB). Three 5-mm segments were analyzed; the proximal MV (MVp), distal MV (MVd) and SB ostium (SBo). For each segment, volumetric analysis was performed in each of four quadrants (divided according to the branch takeoff and the geometric center of the lumen); carinal, epicardial, abcarinal, and myocardial. The eccentricity index was defined as the ratio of the abcarinal plaque (or neointimal) volume to the carinal plaque (or neointimal) volume.

Results
The plaque distribution differed significantly between the four quadrants, with the largest in the abcarinal quadrant, followed by the myocardial, epicardial, and carinal quadrants. The distribution of neointima was similar in the MV, but the four quadrants in the SB did not differ significantly. The eccentricity indices of both the MVd (P <0.001) and SBo (P=0.001) were significantly higher for the plaque group than the neointima group.

Conclusions
The distribution of neointimal proliferation seems to have a similar pattern to that of atherosclerotic plaque in native coronary arteries, particularly in the main vessel, but the trend is less prominent.
Can “Hybrid stent implantation” improve long-term safety without adversely affecting efficacy when treating multilesion coronary artery disease in the drug-eluting stent era?
Authors: ZHANG Dong , XU Bo , DOU Ke-fei , YANG Yue-jin , CHEN Ji-lin , QIAO Shu-bin , WANG Yang , LI Jian-jun , QIN Xue-wen , YAO Min , LIU Hai-bo , WU Yong-jian , CHEN Jue , YUAN Jin-qing , YOU Shi-jie , LI Wei , GAO Run-lin 
Published: January 2024

Background
Though drug-eluting stent (DES) almost solved a problem of restenosis, safety issues related to stent thrombosis are still the major concern of DES. We hypothesized that hybrid stent implantation may decrease the use of DES, probably improving the long-term safety but not affecting efficacy adversely when treating multilesion coronary artery disease in the DES era.

Methods
From April 2004 to October 2006, 848 patients with multilesion disease underwent hybrid stent implantation. During the same period 5647 patients with multilesion coronary heart disease were treated by exclusive DES implantation in Fu Wai Hospital. According to propensity score matching, we chose 823 pairs of patients with multileison coronary artery disease for inclusion into our study. We obtained the 24-month clinical outcome including death, myocardial infarction (MI), thrombosis, target lesion revascularization (TLR), target vessel revascularization (TVR), and major adverse cardiac events (MACE, the composite of death, MI, and TVR). We used Cox's proportional-hazard models to assess relative risks of all the outcome measures after propensity match.

Results
At 24 months, patients in the hybrid stent implantation group showed a significantly higher risk of TLR (8.39% vs. 3.28%, HR 2.38, 95% CI: 1.50-3.70), TVR (11.07% vs. 6.32%, HR 1.61, 95% CI: 1.15-2.27) and MACE (13.75% vs. 8.75%, HR 1.37, 95% CI: 1.02-1.85). No significant difference was apparent in terms of mortality (1.22% vs. 1.70%, HR 0.55, 95% CI: 0.24-1.25), MI (1.95% vs. 2.31%, HR 0.73, 95% CI: 0.37-1.42), or thrombosis (definite+probable) (0.73% vs. 1.58%, HR 0.40, 95% CI: 0.15-1.05).

Conclusions
In patients with multilesion coronary artery disease, the exclusive DES implantation was associated with significantly lower risks of TLR, TVR and MACE, and the hybrid stent implantation did not result in any significant improvements regarding safety issues. Prospective studies are needed to confirm our results.
Molecular mechanism of indirubin-3′-monoxime and Matrine in the reversal of paclitaxel resistance in NCI-H520/TAX25 cell line
Authors: LUO Su-xia , DENG Wen-ying , WANG Xin-feng , Hui-fang LÜ , HAN Li-li , CHEN Bei-bei , CHEN Xiao-bing , LI Ning 
Published: January 2024

Background
Multidrug resistance (MDR) is a main reason for paclitaxel (TAX) treatment failure. Indirubin-3′-monoxime (IRO) and Matrine are traditional Chinese medicines, which may reverse the resistance of tumor cells to some chemotherapy drugs, but the relationship between paclitaxel resistance and Matrine is still unclear. The aim of this study was to explore the potential molecular mechanism of IRO and Matrine in reversal of TAX resistance.

Methods
In this study, MTT assay was used to measure the non-cytotoxic dosage of IRO and Matrine on NCI-H520/TAX25 cells and determine the reversal extent of TAX resistance under non-toxic doses. In addition, RT-PCR and Western blotting were used to evaluate the mRNA expression and the protein level of survivin, Oct-4, and Sox-2 in NCI-H520/TAX25 cells using semi-quantitative methods.

Results
There was no obvious inhibition on sensitive cell strains and drug-resistant strains, when the final concentration was at lest 4 μmol/L for IRO and 100 μmol/L for Matrine. So 4 μmol/L of IRO and 100 μmol/L of Matrine were considered as the reversal dosage. When 4 μmol/L of IRO or 100 μmol/L of Matrine were used together with TAX, the sensitivity to TAX increased evidently in NCI-H520/TAX2 cells; the reversal rate of IRO and Matrine was about 1.92 (43.56/22.6 nmol/L) and 1.74 (43.56/25.0 nmol/L), respectively. The mRNA expression and the protein level of survivin, Oct-4, and Sox-2 in NCI-H520/TAX25 decreased significantly (P <0.05) after addition of IRO or Matrine in TAX treatment, compared to that of TAX treatment alone.

Conclusion
The decrease in both mRNA expression and protein level of survivin, Oct-4, and Sox-2 might be the molecular mechanism, by which IRO and Matrine mediate the reversal of TAX resistance.
Elective single blastocyst transfer is more suitable for normal responders than for high responders
Authors: WU Ke-liang , ZHAO Hai-bin , LIU Hui , ZHONG Wan-xia , YU Guan-ling , CHEN Zi-jiang 
Published: January 2024

Background
Embryo quality and receptivity of the endometrium are two factors that determine the results of in vitro fertilization/intra-cytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET). There is no consensus of the optimal transfer strategy for normal responders or high responders. The current study aimed to find the optimal transfer strategy for different subgroups of patients.

Methods
From April 2010 to December 2010, patients who meet the following criteria were included in this study; primary infertility, female age <35 years, FSH level on female cycle day 2-3 ≤12 mIU/ml, at least six good quality embryos available on day three.The clinical outcomes using different transfer strategies between normal responders and high responders were reviewed and compared.

Results
For the normal responders, the clinical pregnancy rate of day three double-embryo transfer (DET) was comparable to that of day five elective single blastocyst transfer (eSBT), 64.04% vs. 60.33% (P>0.05). For the high responders, the clinical pregnancy rate of day five eSBT was significantly lower than that of day three DET, 43.35% vs. 57.21% (P<0.05). For the high responders, the rates of clinical pregnancy and implantation in frozen-thawed embryo transfer (FET) cycles were notably higher than in eSBT cycles (64.56% vs. 43.35% and 62.11% vs. 43.35% respectively) (P<0.05).

Conclusions
For normal responders, eSBT might be an applicable strategy to reduce multiple pregnancy rates while maintaining acceptable overall pregnancy rates. And in order to reduce multiple pregnancies and increase the chance of pregnancy of high responders, FET may be a preferable strategy.
Change of CMTM7 expression, a potential tumor suppressor, is associated with poor clinical outcome in human non-small cell lung cancer
Authors: LIU Qiang , SU Yu , JIANG Guan-chao , ZHOU Zu-li , LIU Bao-cai , BU Liang , YANG Fan , QIAO Fang , WANG Jun 
Published: January 2024

Background
CKLF-like MARVEL transmembrane domain-containing 7 (CMTM7) located at 3p22.3, is a frequent deletion site and a tumor suppressor gene (TSG) locus in many cancer, which suggests CMTM7 may be a potential TSG. The aim of this study was to investigate the correlations of CMTM7 expression and survival rate in patients with non-small-cell lung cancer (NSCLC).

Methods
Surgical specimens of 180 cases with pathologically confirmed NSCLC were grouped into 18 tissue microarray slides. CMTM7 expression in these specimens were detected by immunohistochemistry staining and representative cases were confirmed by Western blotting. Univariate and multivariate analyses were performed to identify the association of CMTM7 expression with pathological features and survival of patients with NSCLC.

Results
A total of 78.9% of the 180 patients had variations of CMTM7 protein expression, either up-regulated or down-regulated. Univariate analysis showed that the patients' survival rate after surgery was highly correlated with CMTM7 expression (P=0.0091). In addition, prognostic factors were examined by multivariate Cox regression analysis, and results suggested that CMTM7 expression was a unique prognostic factor in NSCLC survival.

Conclusions
The CMTM7 expression may be related to survival of patients with NSCLC and a unique prognostic factor. CMTM7 may play an important role in NSCLC development.
A single-site retrospective study of pediatric arterial ischemic stroke etiology, clinical presentation, and radiologic features
Authors: SUN Dan , WU Xiao-man , WANG Zeng-wu , JIN Run-ming , LIU Zhi-sheng , LIU Fan , HUANG Sui , WANG Hai-qin , HU Jia-sheng 
Published: January 2024

Background
Stroke occurs upon obstruction of cerebral blood circulation and is clinically characterized by sudden onset symptoms. Advanced age is the main risk factor of stroke, but cases of pediatric stroke have been rarely reported. This study aimed to determine the etiology, clinical presentation, and radiologic features of neurological deficit for pediatric arterial ischemic stroke (PAIS).

Methods
The medical records of 42 PAIS patients (age range: 9 months to 13 years) treated at Wuhan Children's Hospital between July 2007 and January 2011 were retrospectively reviewed. Infarction location was first determined by craniocerebral computed tomography and magnetic resonance (MR) imaging. The stenotic or occluded main cerebral arteries and/or branches were determined by MR angiography and digital subtraction angiography.

Results
The majority of the 42 PAIS cases (66.7%, n=28) were ≤3 years old (vs. >3 years old: 33.3%, n=14; P<0.05), but the male: female ratio was similar in both groups (P>0.05). The most frequently reported signs and symptoms for both age groups were limited physical activity followed by convulsions and delirium, but convulsions were more prevalent in children ≤3 years-old. Children>3 years-old mainly experienced the limited physical activity symptoms, including hemiparalysis, aphasia, and ataxia. For all 42 cases, the most frequent etiologies were infections (38.1%, n=16), iron deficiency anemia (16.7%, n=7), and moyamoya syndrome (11.9%, n=5). The predominant infarcts among all cases were middle cerebral artery (63.6%, n=21) and basal ganglia (64.3%, n=27).

Conclusions
PAIS occurs more frequently in younger children and this group most frequently presents with convulsion as the initial symptom. The overall etiologies of PAIS may be different from those of adult stroke and the involved regions may be distinguishing features of PAIS or its different forms, but more research is required.