bluuebunny/crossref_metadata_2025_split huggingface dataset:
Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis
Authors: Zhou Huan , Yuan Wang , Lei Weiqiang , Zhou Taoxun , Qin Peixi , Zhang Biying , Hu Min 
Published: November 2023

Abstract
Background
Ribosome biogenesis is the process of assembling ribosome complexes that regulate cell proliferation and differentiation with potential regulatory effects on development. Many factors regulate ribosome biological processes. Nin one binding protein (Nob1) has received widespread attention as key genes regulating ribosome biogenesis—the 3ʹ end of the 20S rRNA is cleaved by Nob1 at cleavage site D to form 18S rRNA, generating translationally capable 40S subunit. As a ribosome biogenesis factor, Nob1 may regulate the development of organisms, but almost nothing is known about the function of Nob1 for any parasitic nematode. We explored the functional role of NOBP-1 (the homologous gene of Nob1) encoding gene from a parasitic nematode—Strongyloides stercoralis.

Methods
The full-length cDNA, gDNA and promoter region of Ss-nobp-1 was identified using protein BLAST in WormBase ParaSite according to the Caenorhabditis elegans NOBP-1 sequence to analyze the gene structure. RNA sequencing (RNA-seq) data in wormbase were retrieved and analyzed to assess the transcript abundance of Ss-nobp-1 in seven developmental stages of S. stercoralis. The standard method for gonadal microinjection of constructs was carried out to determine the anatomic expression patterns of Ss-nobp-1. The interaction between Ss-NOBP-1 and partner of NOBP-1 (Ss-PNO-1) was assessed by yeast two-hybridization and bimolecular fluorescence complementarity (BiFC) experiments.

Results
The NOBP-1 encoding gene Ss-nopb-1 from the zoonotic parasite S. stercoralis has been isolated and characterized. The genomic DNA representing Ss-nobp-1 includes a 1599-bp coding region and encodes a protein comprising 403 amino acids (aa), which contains conserved PIN domain and zinc ribbon domain. RNA-seq analysis revealed that Ss-nobp-1 transcripts are present throughout the seven developmental stages in S. stercoralis and have higher transcription levels in iL3, L3 and P Female. Ss-nobp-1 is expressed mainly in the intestine of transgenic S. stercoralis larvae, and there is a direct interaction between Ss-NOBP-1 and Ss-PNO-1.

Conclusions
Collectively, Ss-NOBP-1 has a potential role in embryo formation and the infective process, and findings from this study provide a sound foundation for investigating its function during the development of parasitic nematode.

Graphical Abstract
Diversity of Thelazia spp. in domestic cattle from Romania: epidemiology and molecular diagnosis by a novel multiplex PCR
Authors: Cotuțiu Vlad-Dan , Ionică Angela Monica , Dan Teodora , Cazan Cristina Daniela , Borșan Silvia Diana , Culda Carla Andreea , Mihaiu Marian , Gherman Călin Mircea , Mihalca Andrei Daniel 
Published: November 2023

Abstract
Background
Thelaziosis is a neglected vector-borne disease caused by parasitic nematode worms of the genus Thelazia which affects various hosts. Limited attention has been given to ungulate-associated Thelazia species. Current diagnosis of thelaziosis and the identification/differentiation of species heavily relies on morphological features. Therefore, we conducted an epidemiological study in Romanian cattle, with the aim to obtain morphological and molecular data that can be used for species identification.

Methods
The eyes of 705 slaughtered cattle were sampled and subjected to morphological identification, morphometric analysis, and molecular characterization. PCR amplification and sequence analysis were performed based on the cytochromec oxidase subunit 1 (COI) gene. Statistical tests assessed the correlations between infection parameters and ecological or biogeographical factors. A novel PCR method was developed based on the consensus sequence from each species. Specific forward primers were designed for each of the three species, and a reverse primer (COIintR) was used for all reactions. A consensus thermal profile was established by gradient PCR amplification of each species separately.

Results
Of the sampled cattle, 19.3% were infected with Thelazia spp. Prevalence varied significantly with ecogeographical factors. A total of 585 Thelazia nematodes were recovered, with T. rhodesi being the most abundant, followed by T. skrjabini and T. gulosa. Morphometric and molecular analyses supported the morphological identification, yielding unique sequences for each species. From the 59 T. rhodesi specimens sequenced, 29 unique sequences were obtained, with a 99.1–99.85% nucleotide identity to the only other COI sequence present in GenBank®. All nine T. gulosa isolates were unique (99.37–100% nucleotide identity to other sequences), while T. skrjabini specimens displayed 98.47–100% nucleotide identity to the sole available sequence.

Conclusions
Bovine thelaziosis is prevalent in Romania, raising concerns for animal welfare and potential economic impacts. Infected cattle grazing alongside vulnerable wild ruminants, such as the European bison, may affect conservation efforts. Our newly developed multiplex PCR shows promise as a valuable surveillance tool, enabling the detection of occult infections in apparently healthy animals through lachrymal secretion testing.

Graphical Abstract
Investigating the association between characteristics of local crisis care systems and service use in an English national survey
Authors: Rojas-García Antonio , Dalton-Locke Christian , Sheridan Rains Luke , Dare Ceri , Ginestet Cedric , Foye Una , Kelly Kathleen , Landau Sabine , Lynch Chris , McCrone Paul , Nairi Shilpa , Newbigging Karen , Nyikavaranda Patrick , Osborn David , Persaud Karen , Sevdalis Nick , Stefan Martin , Stuart Ruth , Simpson Alan , Johnson Sonia , Lloyd-Evans Brynmor 
Published: November 2023

Background
In England, a range of mental health crisis care models and approaches to organising crisis care systems have been implemented, but characteristics associated with their effectiveness are poorly understood.

Aims
To (a) develop a typology of catchment area mental health crisis care systems and (b) investigate how crisis care service models and system characteristics relate to psychiatric hospital admissions and detentions.

Method
Crisis systems data were obtained from a 2019 English national survey. Latent class analyses were conducted to identify discernible typologies, and mixed-effects negative binomial regression models were fitted to explore associations between crisis care models and admissions and detention rates, obtained from nationally reported data.

Results
No clear typology of catchment area crisis care systems emerged. Regression models suggested that provision of a crisis telephone service within the local crisis system was associated with a 11.6% lower admissions rate and 15.3% lower detention rate. Provision of a crisis cafe was associated with a 7.8% lower admission rates. The provision of a crisis assessment team separate from the crisis resolution and home treatment service was associated with a 12.8% higher admission rate.

Conclusions
The configuration of crisis care systems varies considerably in England, but we could not derive a typology that convincingly categorised crisis care systems. Our results suggest that a crisis phone line and a crisis cafe may be associated with lower admission rates. However, our findings suggest crisis assessment teams, separate from home treatment teams, may not be associated with reductions in admission and detentions.
A New History of ‘Made in Italy’: Fashion and Textiles in Post-War Italy, Lucia Savi (2023)
Authors: Hart Rachel 
Published: November 2023

Review of: A New History of ‘Made in Italy’: Fashion and Textiles in Post-War Italy, Lucia Savi (2023)
London: Bloomsbury Publishing Plc, 204 pp.,
ISBN 978-1-35024-7758, h/bk, £85
Effects of ZnO nanoparticles, polyethylene glycol 400, and polyoxyethylene sorbitan ester Tween 80 on PLA films properties
Authors: Tajari Neda , Sadrnia Hassan , Hosseini Fereshte 
Published: November 2023

Abstract
Polylactic acid has the potential to be an alternative to petroleum-based materials in the food packaging industry. In this study, the effect of zinc oxide nanoparticles, polyethylene glycol 400, and Tween 80 as plasticizers on the properties of polylactic acid films was investigated. In order to study the mechanical properties more accurately, the tests were repeated for four months. An experimental design method was used to investigate the effects of additives on the measurement factors and finally to choose the optimal combination with the help of the TOPSIS technique. The results showed that the addition of different materials increased the opacity, most of which being related to P400/ZnO (6.82 ± 0.07 mm−1). The presence of plasticizers increases the hydrophilicity of the film and the water vapor permeability. The highest contact angle (85.33° ± 4.00) and the lowest water vapor permeability (0.074 ± 0.002 g mm/kPa h m2) were related to neat PLA film. The lowest modulus of elasticity, the highest tensile strength and elongation at break were related to P400/T80/ZnO (1.18 ± 0.17 GPa, first month), PLA/ZnO (96.28 ± 3.17 MPa, fourth month), and P400/ZnO (76.82 ± 27.22 %, first month), respectively. The effect of plasticizers was significant in most of the measurement factors, but the effect of nanoparticles was significant in some cases such as opacity and contact angle. The results of an ANOVA analysis showed that the effect of film type on the mechanical properties was significant, and the effect of storage time was only significant on the elongation at break. According to the results obtained from the TOPSIS technique, P400/ZnO was chosen as the combination with the best features among the produced films.
La educación inicial con una perspectiva hacia el emprendimiento en el contexto rural
Authors: Burbano Jiménez Neyit Sveidy , Molina Burbano Jessica Dayana , Castro Juan José 
Published: November 2023

El acercamiento del ser humano hacia su propio entorno le ayuda a descubrir la esencia que lo rodea, a la vez que contribuye en el desarrollo de las dimensiones que integran la individualidad de la persona. En el presente artículo se hace un análisis de la importancia de la educación trabajada desde el contexto donde está ubicado el ser que aprende y que enseña, tomando como referencia las experiencias vividas desde la práctica pedagógica de los autores en la búsqueda de favorecer las habilidades de emprendimiento de los niños. El estudio referido en el artículo se adelantó con estudiantes del nivel preescolar y básica primaria del sector rural, en donde se dispone de los recursos proporcionados por el entorno y que permiten dar significado al aprendizaje.
La responsabilidad social
Authors: Benavides Paz Nelson Felipe 
Published: November 2023

La Responsabilidad Social en las Organizaciones nace a comienzos del siglo XX con el concepto denominado filantropía, el cual se sustenta en acciones esporádicas por parte de las estructuras organizacionales a favor de una causa contextual, sin un seguimiento o estrategia empresarial transversal.
Entre los años 50‘s y 60‘s ese concepto se desarrolla hasta empezar a hablar de estrategia y se comienza a generar la noción de Responsabilidad Social [RS], la cual debe ser gestionada para considerarse estratégica; es así como la RS debe ser una obligación a fin de que pueda ser valorada como sustentable y aporte al desarrollo sostenible de los contextos mundiales.
La vida desde el heterodoxo cósmico
Authors: Rojas Vergara Emma Del Pilar 
Published: November 2023

Hablar de la vida, hablar del hombre, hablar del hombre heterodoxo en la realidad donde se hace, se forma y existe, es el ejercicio permanente del hombre que se mira a sí mismo, reconoce su entorno y descubre el sentido de su vida. Esta es una comprensión que conlleva a que el hombre se aproxime cada vez a una mayor profundización en el conocimiento de sí mismo, de los demás y del espacio donde transcurre el milagro permanente de la existencia.
Desde la Filosofía tenemos la oportunidad de explorar este grandioso horizonte para el pensamiento de los heterodoxos de hoy, propósito que se esboza en el artículo a partir del compartir el ejercicio investigativo doctoral en Filosofía.
El erotismo, parte de la cotidianidad que muchos prefieren callar
Authors: Huertas Timaná Laura Sofía , Yamá Diana Lucía , Timaná de la Cruz Alba Yamile 
Published: November 2023

El erotismo, es una característica propia del ser humano, prácticamente ineludible desde sus orígenes. No obstante, ha sido invisibilizado y menospreciado por la sociedad, a tal punto de ser un tema prohibido hasta la actualidad. Debido a esto, con el presente escrito se pretende cavilar a la luz de diferentes autores sobre el tema, de tal forma que se lo considere y reconozca como una dimensión propia de la existencia de cada individuo, que no puede recaer únicamente en lo biológico y fisiológico enmarcado en el placer, lo inmoral y obsceno, sino dar la posibilidad de entenderlo como una forma en que el cuerpo se expresa, no solo desde el apetito carnal, sino también espiritual, el autorreconocimiento y el respeto por el otro, como resultando de una forma amplia de lenguaje.
Elevated serum apolipoprotein E level associated with poor prognosis of patients with acute omicron variant COVID-19 infection
Authors: Shi Cong , Ma Chao , Ma Ruishuang , Zhu Ting , Ge Xiaoqin , Xie Rongrong , Tao Qingsong , Gong Shengping 
Published: November 2023

Abstract
Background: The SARS-CoV-2 virus is responsible for the human disease known as COVID-19. This virus is capable of generating a spectrum of infections ranging from moderate to severe. The omicron variant is currently the predominant mutant strain of the pandemic on a global scale. Serum apolipoprotein E (ApoE) inhibits inflammation by preserving immune regulatory function. Nonetheless, the relationship between serum ApoE and clinical prognosis in omicron remains elusive.
Methods: A cohort of 231 patients with omicron was studied retrospectively to determine the prognostic usefulness of blood ApoE levels upon diagnosis. All patients were observed for 65 days, with death as the primary outcome. Based on their ApoE levels, the patients were categorized into two groups: individuals with elevated ApoE levels and those with lower ApoE levels. In order to do statistical comparisons, the log-rank test was utilized, and the Kaplan-Meier method was utilized in order to estimate survival rates. Cox hazard models, both univariate and multivariate, were employed to examine the prognostic relevance.
Results: According to our research, omicron had significantly greater ApoE levels. In mild-to-moderate and severe cases, the study identified a statistically significant variation in ApoE levels. Procalcitonin (PCT), Interleukin-6 (IL-6) and Interleukin-10 (IL-10) levels were found to be considerably higher, and the absolute count of CD3 lymphocyte, absolute count of CD8 lymphocytes was found to be fewer in people with high levels of ApoE. Additionally, there was a drop in overall survival that is statistically significant (OS, P < 0.0001) for patients with greater ApoE levels. Multiple Cox proportional hazards regression analysis indicates that (P = 0.022), an elevated ApoE level, specifically more than 49.8 mg/L, was determined to be an adverse and independent prognostic factor of overall survival in patients with omicron.
Conclusion: The level of serum ApoE at the time of initial diagnosis was substantially connected to the severity and prognosis of omicron. Consequently, we propose that ApoE might be a poor prognostic factor in individuals afflicted with the omicron variant.
Hippopotamus Optimization Algorithm: A Novel Nature-Inspired Optimization Algorithm
Authors: Amiri Mohammad Hussein , Hashjin Nastaran Mehrabi , Montazeri Mohsen , Mirjalili Seyedali , Khodadadi Nima 
Published: November 2023

Abstract
The novelty of this article lies in introducing a novel nonparametric metaheuristic technique named the Hippopotamus Optimization (HO) algorithm. The HO is conceived by drawing inspiration from the inherent behaviors observed in hippopotamuses, showcasing an innovative approach in metaheuristic methodology. The HO is conceptually defined using a trinary-phase model that incorporates their position updating in rivers or ponds, defensive strategies against predators, and evasion methods, which are mathematically formulated. It attained the top rank in 132 out of 161 benchmark functions in finding optimal value, encompassing unimodal and high-dimensional multimodal functions, fixed-dimensional multimodal functions, as well as the CEC 2019 test suite and CEC 2014 test suite dimensions of 10, 30, 50, and 100 and Zigzag Pattern benchmark functions, this suggests that the HO demonstrates a noteworthy proficiency in both local search and exploitation, as well as in global search and exploration. Moreover, it effectively balances exploration and exploitation, supporting the search process. The performance of the HO consistently surpassed that of the top 3 algorithms in achieving optimal value, except for 29 functions. However, although it did not exhibit strong convergence in these 29 functions, the standard deviation for them was lower than the other investigated algorithms, illustrating its ability to manage the functions effectively. In light of the results from addressing four distinct engineering design challenges, the HO has effectively achieved the most efficient resolution while concurrently upholding adherence to the designated constraints. The Wilcoxon signed test demonstrates that HO exhibits a notable and statistically significant advantage over the investigated algorithms in effectively addressing the optimization problems examined in this study.
Whole transcriptome sequencing analysis revealed key RNA profiles in primary Sjögren's syndrome
Authors: Wang Guangyu , Liu Zhenyu , Zhang Junning , Yang Xueli , Feng Zhihui , Zhang Biao , Pan Xiaoping , Li Chunhong , Tang Yuhan , Dai Yong , Hou Xianliang 
Published: November 2023

Abstract
Background
The pathogenesis of Primary Sjögren's syndrome (pSS) is complex and no definite conclusions have been reached. Accumulating evidence suggests that non-coding RNAs (ncRNAs) are involved in the pathogenesis of pSS. However, regulatory mechanisms of ncRNAs in pSS are not fully elucidated, including mRNAs, circRNAs, lncRNAs and miRNAs.
Results
In this study, whole blood samples of 3 pSS patients and 3 healthy individuals were collected, which were sequenced by whole transcriptome sequencing to identify differentially expressed mRNAs, lncRNAs, circRNAs, and miRNAs. Compared to the healthy control group, we identified 205 differentially expressed mRNAs (DEMs), 543 DE lncRNAs (DELs), 28 DE circRNAs (DECs) and 18 DE miRNAs (DEmiRs) in the pSS group. In addition, GO and KEGG analysis showed that these differentially expressed genes were mainly enriched in the PI3K-Akt signaling pathway, NF-kappa B signaling pathway, B cell receptor signaling pathway, calcium signaling pathway, ECM-receptor activation and VEGF signaling pathway. Furthermore, the constructed ceRNA networks suggested that several miRNAs might be a crucial role in the pathogenesis of pSS.
Conclusions
These results provide date support for exploring the key ncRNAs and signaling pathways in pSS. The mRNA-miRNA-lncRNA/circRNA network may contribute to the insight into the pathogenesis of disease.
Syntheses and Biological Activities of Calix[4]resorcinarene Derivatives Modified by Sulfonamides
Authors: Wang Qing , Sun Meng , Zhou Xin-Min , Ye Xiao-Dan , Jia Ai-Quan , Zhang Qian-Feng 
Published: November 2023

Abstract
Calixarenes are a diverse class of macrocyclic compounds, and the calix[4]resorcinarenes studied in this paper are one of the special subgroups. The calix[4]resorcinarene derivatives with the introduction of sulfonic and sulfonamides groups at C-2 positions were synthesized and characterized by IR and 1H NMR spectroscopies. The compounds synthesized in the paper were used for antibacterial and antitumor tests. The results showed that in terms of antimicrobial activity calix[4]resorcinarenes and their derivatives showed bacteriostatic activity against both Escherichia coli and Staphylococcus aureus, with compound 4c being the most effective: the MIC value for E. coli was 6.25 mg/mL and for S. aureus was 1.56 mg/mL. In terms of antitumor activity, calix[4]resorcinarenes and their derivatives showed strong inhibitory effects on the three tumor cells selected for the experiment. Among them, the survival rate of A549 was 76.03% in the presence of 40 µM 1d, and the survival rates of HepG2 and MDA-MB-321 were 28.66% and 65.39% in the presence of 40 µM 2d, respectively.
Diurnal dynamics of different circadian transcription modules in Chinese Pine needles and roots during dormancy introduction
Authors: Yang Junhe , Qu Kai , Wang Huili , Bian Liming , El-Kassaby Yousry A. , Li Wei 
Published: November 2023

Abstract
Winter dormancy ensures temperate zone’s trees proper response to environmental changes and enhances their adaptability. In northern hemisphere, conifers’ dormancy is induced by short-day and cold temperature. Previous studies have revealed that TFL2 is a key regulator involved in conifers’ bud set and growth cessation during the dormancy-induced phase. Based on the annual expression profile analysis of PtTFL2 in Chinese pine (Pinus tabuliformis Carr.), we identified key time nodes for dormancy initiation in autumn. To provide insight of the diurnal transcriptome dynamic in needles and roots during dormancy introduction, RNA-seq was performed at 12 consecutive time points in 24 hours under natural environment in Chinese pine. Interestingly, we found that both needles and roots have rhythmic oscillatory genes, even though the roots could not receive light signals directly. We constructed the transcription factor (TF) co-expression networks of needles and roots and in the core TF modules, we identified 10 hub genes in each. Revealed a network associated with the putative core clock genes (PtCCA1, PtPRR4, PtGI, and PtTCP42), which were also isolated, suggesting that they are important in the circadian regulation of the transcriptome. Regulatory network analysis identified key TFs associated with dormancy and cold acclimation in two tissues. Our results provide new insights into the molecular regulatory mechanisms involved in pine dormancy.
Bioactive Natural Leads and Traditional Herbal Plants in the Management of Inflammatory Bowel Diseases: A Brief Review
Authors: Chauhan Sonia , Sharma Sakshi , Mazumder Rupa , Sharma Nidhi 
Published: November 2023

:
Inflammatory bowel disease is a chronic relapsing disorder that causes chronic inflammation
and ulcers in the GIT. Depending upon the location, ulcerative colitis and Crohn's disease come
under IBD. The exact etiology of IBD is still unknown. Over 8 lakhs of people were affected by
inflammatory disease yearly, and the death rate increased daily. Depending upon the severity of the
disease, JAK inhibitors, anti-TNF agents, and immunosuppressants can be used to manage ulcerative
colitis and Crohn's disease. However, these treatments have been associated with harmful adverse
effects, which cannot be ignored. To treat inflammatory diseases safely, various herbal medicines
and their bioactive are preferred as game changers. Recently, the effectiveness of herbal plants has
been recommended as the treatment against IBD, as shown by various in vivo models and clinical
trials. The various herbal plants reported in the literature include gallic acid, lupeol, and curcumin
aloe vera. This review focused on medicinal plants' anti-inflammatory, antioxidant, and anti-ulcer
properties. Over 1.2 million healthcare practitioners are using herbal bioactive and have the advantages
of lower side effects. Therefore, it is estimated that in Europe, the demand for plant-based
products/formulations has risen by millions in 2020, showing the current position of herbal-based
products in consumer health awareness.