bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cache Valley virus: an emerging arbovirus of public and veterinary health importance
Authors: Hughes Holly R , Kenney Joan L , Calvert Amanda E 
Published: October 2023

AbstractCache Valley virus (CVV) is a mosquito-borne virus in the genus Orthobunyavirus (Bunyavirales: Peribunyaviridae) that has been identified as a teratogen in ruminants causing fetal death and severe malformations during epizootics in the U.S. CVV has recently emerged as a viral pathogen causing severe disease in humans. Despite its emergence as a public health and agricultural concern, CVV has yet to be significantly studied by the scientific community. Limited information exists on CVV’s geographic distribution, ecological cycle, seroprevalence in humans and animals, and spectrum of disease, including its potential as a human teratogen. Here, we present what is known of CVV’s virology, ecology, and clinical disease in ruminants and humans. We discuss the current diagnostic techniques available and highlight gaps in our current knowledge and considerations for future research.
Emerging tickborne viruses vectored by Amblyomma americanum (Ixodida: Ixodidae): Heartland and Bourbon viruses
Authors: Dupuis Alan P , Lange Rachel E , Ciota Alexander T 
Published: October 2023

Abstract
Heartland (HRTV) and Bourbon (BRBV) viruses are newly identified tick-borne viruses, isolated from serious clinical cases in 2009 and 2014, respectively. Both viruses originated in the lower Midwest United States near the border of Missouri and Kansas, cause similar disease manifestations, and are presumably vectored by the same tick species, Amblyomma americanum Linnaeus (Ixodida: Ixodidae). In this article, we provide a current review of HRTV and BRBV, including the virology, epidemiology, and ecology of the viruses with an emphasis on the tick vector. We touch on current challenges of vector control and surveillance, and we discuss future directions in the study of these emergent pathogens.
Everglades virus: an underrecognized disease-causing subtype of Venezuelan equine encephalitis virus endemic to Florida, USA
Authors: Burkett-Cadena Nathan D , Fish Durland , Weaver Scott , Vittor Amy Y 
Published: October 2023

Abstract
Everglades virus (EVEV) is subtype II of the Venezuelan equine encephalitis virus (VEEV) complex (Togaviridae: Alphavirus), endemic to Florida, USA. EVEV belongs to a clade that includes both enzootic and epizootic/epidemic VEEV subtypes. Like other enzootic VEEV subtypes, muroid rodents are important vertebrate hosts for EVEV and certain mosquitoes are important vectors. The hispid cotton rat Sigmodon hispidus and cotton mouse Peromyscus gossypinus are important EVEV hosts, based on natural infection (virus isolation and high seropositivity), host competence (experimental infections), and frequency of contact with the vector. The mosquito Culex (Melanoconion) cecedei is the only confirmed vector of EVEV based upon high natural infection rates, efficient vector competence, and frequent feeding upon muroid rodents. Human disease attributed to EVEV is considered rare. However, cases of meningitis and encephalitis are recorded from multiple sites, separated by 250 km or more. Phylogenetic analyses indicate that EVEV is evolving, possibly due to changes in the mammal community. Mutations in the EVEV genome are of concern, given that epidemic strains of VEEV (subtypes IAB and IC) are derived from enzootic subtype ID, the closest genetic relative of EVEV. Should epizootic mutations arise in EVEV, the abundance of Aedes taeniorhynchus and other epizootic VEEV vectors in southern Florida provides a conducive environment for widespread transmission. Other factors that will likely influence the distribution and frequency of EVEV transmission include the establishment of Culex panocossa in Florida, Everglades restoration, mammal community decline due to the Burmese python, land use alteration by humans, and climate change.
The known unknowns of Powassan virus ecology
Authors: Brackney Doug E , Vogels Chantal B F 
Published: October 2023

Abstract
Powassan virus (POWV; Family: Flaviviridae, Genus: Flavivirus) is the sole North American member of the tick-borne encephalitis sero-complex. While associated with high rates of morbidity and mortality, POWV has historically been of little public health concern due to low incidence rates. However, over the last 20 yr, incidence rates have increased highlighting the growing epidemiological threat. Currently, there are no vaccines or therapeutics with tick habitat reduction, acaricide application, and public awareness programs being our primary means of intervention. The effectiveness of these control strategies is dependent on having a sound understanding of the virus’s ecology. In this Forum, we review what is currently known about POWV ecology, identify gaps in our knowledge, and discuss prevailing and alternative hypotheses about transmission dynamics, reservoir hosts, and spatial focality.
The increasing threat of Rift Valley fever virus globalization: strategic guidance for protection and preparation
Authors: Gibson Seth , Noronha Leela E , Tubbs Heidi , Cohnstaedt Lee W , Wilson William C , Mire Chad , Mitzel Dana , Anyamba Assaf , Rostal Melinda , Linthicum Kenneth J 
Published: October 2023

Abstract
Rift Valley fever virus (RVFV) (Bunyavirales: Phlebovirus) is a prominent vector-borne zoonotic disease threat to global agriculture and public health. Risks of introduction into nonendemic regions are tied to changing climate regimes and other dynamic environmental factors that are becoming more prevalent, as well as virus evolutionary factors and human/animal movement. Endemic to the African continent, RVFV has caused large epizootics at the decadal scale since the early 20th century but has spread to the Arabian Peninsula and shows increasing patterns of interepizootic transmission on the annual scale. This virus can be transmitted by mosquitoes as well as through direct contact with infected tissues and can cause sporadic to widespread morbidity and mortality in domestic ungulate livestock as well as humans. High viremias in infected livestock moved for legal and illegal trade as well as in infected mosquitoes or human travelers can spread this virus worldwide. With increasing global commerce, it is likely RVFV will be introduced to new areas with suitable hosts, mosquito vector species, and environments. However, the strong mosquito component of RVFV epidemiology combined with advancements in vaccines, diagnostics, and virus evolutionary factors create opportunities for strategies to leverage models of connectivity among potential source and emerging regions to target surveillance and mitigation activities to reduce the risk of RVFV introduction, or contain the virus should it be introduced, into new regions.
Temporal population genetic structure of Phormia regina (Diptera: Calliphoridae)
Authors: Owings Charity G , Banerjee Aniruddha , Picard Christine J 
Published: October 2023

Abstract
The genetic structure of forensically important blow fly (Brauer & Bergenstamm) (Diptera: Calliphoridae) populations has remained elusive despite high relatedness within wild-caught samples. This research aimed to determine if the implementation of a high-resolution spatiotemporal sampling design would reveal latent genetic structure among blow fly populations and to elucidate any environmental impacts on observed patterns of genetic structure. Adult females of the black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae), were collected from 9 urban parks in Indiana, USA over 3 yr and genotyped at 6 polymorphic microsatellite loci. The data analysis involved 3 clustering methods: principal coordinate analysis (PCoA), discriminant analysis of principal components (DAPC), and STRUCTURE. While the PCoA did not uncover any discernible clustering patterns, the DAPC and STRUCTURE analyses yielded significant results, with 9 and 4 genetic clusters, respectively. Visualization of the STRUCTURE bar plot revealed N = 11 temporal demarcations indicating barriers to gene flow. An analysis of molecular variance of these STRUCTURE-inferred populations supported strong temporally driven genetic differentiation (FST = 0.048, F’ST = 0.664) relative to geographic differentiation (FST = 0.009, F’ST = 0.241). Integrated Nested Laplace Approximation and Boosted Regression Tree analyses revealed that collection timepoint and 4 main abiotic factors (temperature, humidity, precipitation, and wind speed) were associated with the genetic subdivisions observed for P. regina. A complex interplay between environmental conditions, the unique reproductive strategies of the blow fly, and the extensive dispersal abilities of these organisms likely drives the strong genetic structure of P. regina in the Midwestern US.
National Cancer Institute funding for rapid cycle interventional research in cancer care delivery
Authors: Norton Wynne E , Neta Gila , Jacobsen Paul B 
Published: October 2023

Abstract

Background
Rapid cycle interventional research can accelerate improvements to cancer care delivery and patient health outcomes by answering multiple questions as part of a single research study. To complement ongoing efforts to increase awareness of and support for rapid cycle interventional research, we conducted a systematic portfolio analysis of research grants funded by the National Cancer Institute on the topic.

Methods
We used standard portfolio analytic methods for identifying, coding, and synthesizing rapid cycle interventional research funded by the National Cancer Institute between 2016 and 2022. A codebook was used to standardize assessment of the grants by common study characteristics, intervention topics, and cancer care delivery context.

Results
We identified 26 grants, mostly funded since 2019, as rapid cycle interventional research. Most studies included adult or older adult target populations, used electronic systems for intervention delivery, and focused primarily on testing different components of interventions. Studies also used a range of study designs, intervention content areas, cancer sites, and across the cancer control continuum.

Conclusions
The current portfolio analysis of funded rapid cycle interventional research grants suggests a growing albeit relatively small number of studies in this area. Several efforts are needed to continue to grow this area of research, including training programs, funding opportunities, and strengthening research-practice partnerships. This analysis provides a snapshot of current studies and highlights the opportunity for growing this important area of research to optimize cancer care delivery and improve patient outcomes.
An Analysis of Micro-scale Conflict in Collaborative Governance
Authors: Ulibarri Nicola 
Published: October 2023

Abstract
Conflict is the forgotten sibling of collaborative governance. Variably framed as an alternative to collaboration, a contextual feature shaping interpersonal interactions, or an obstacle to be overcome via deliberation, conflict lurks in the background of discourse about collaboration. However, few theories of collaboration directly address the role of conflict, and those that do focus on conflict as a macro-scale phenomenon, characteristic of a governance forum or participating organizations. Given the importance of short term, person-to-person interactions in shaping the overall trajectory of collaborative dynamics and outcomes, a micro-scale analysis of collaborative conflict is warranted. This article develops a framework for evaluating the role of micro-scale conflict in collaborative governance, drawing on the case of negotiations to relicense hydropower dams in the Central Valley of California, USA. Data sources include 4 years of meeting observations, interviews with participating stakeholders, and written comments submitted during the process. The work first classifies all instances of disagreement observed during the negotiations to develop a typology of micro-scale conflict. It then compares differences in the frequency, type, and management of disagreements in high and low collaboration relicensings to explore the interaction between conflict dynamics and overall collaborative approach. In the high collaboration case, interpersonal disagreements occurred frequently, were more dynamic and mutable over time, and served to elaborate and refine management approaches. By evaluating conflict dynamics that occur at the scale of an individual interaction and the positive and negative roles they play in shaping collaborative outcomes, this research moves conflict from being a static barrier or contextual factor to a dynamic ingredient that can be managed to shape policy outcomes.
A paradoxical internal hernia at Brolin’s anti-obstruction stitch following Roux-en-Y gastric bypass: a case report
Authors: Patel Krishna , Serapiglia Vinnie , Rizzo Anthony N , Varre Jaya , Sonnanstine Thomas 
Published: October 2023

Abstract
Internal hernias are a rare but morbid complication following Roux-en-Y gastric bypass surgery. The incorporation of Brolin’s anti-obstruction stitch has historically demonstrated a significant reduction in the incidence of internal hernias following Roux-en-Y gastric bypass. We present an ironic and unique case of a patient with small bowel herniation into a defect between Brolin’s stitch and the stapled closed common enterotomy of the jejunojejunostomy and technical considerations to decrease internal hernias at this site in the future.
Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs
Authors: Ye Shengjian , Wang Sheng , Chan Ron , Cao Ling , Wang Hong 
Published: October 2023

Abstract
Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kip-related proteins (KRPs). ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels. However, understanding of how ICK protein levels are regulated is very limited. We fused Arabidopsis ICK sequences to green fluorescent protein (GFP) and determined their effects on the fusion proteins in plants, yeast, and Escherichia coli. The N-terminal regions of ICKs drastically reduced GFP fusion protein levels in Arabidopsis plants. A number of short sequences of 10–20 residues were found to decrease GFP fusion protein levels when fused at the N-terminus or C-terminus. Three of the four short sequences from ICK3 showed a similar function in yeast. Intriguingly, three short sequences from ICK1 and ICK3 caused the degradation of the fusion proteins in E. coli. In addition, computational analyses showed that ICK proteins were mostly disordered and unstructured except for the conserved C-terminal region, suggesting that ICKs are intrinsically disordered proteins. This study has identified a number of short protein-destabilizing sequences, and evidence suggests that some of them may cause protein degradation through structural disorder and instability.
AstroSat observation of the magnetar SGR J1830−0645 during its first detected X-ray outburst
Authors: Sharma Rahul , Jain Chetana , Paul Biswajit , Seshadri T R 
Published: October 2023

ABSTRACT
We present here timing and spectral analyses of SGR J1830−0645 based on an AstroSat observation carried out on 2020 October 16, about a week after the onset of its first detected X-ray outburst. Using data taken with the Soft X-ray Telescope (SXT) and Large Area X-ray Proportional Counter (LAXPC), we have detected 0.9–10 keV coherent pulsations at a period of ∼10.4 s. The pulse profiles were single-peaked, asymmetric, and consisted of minor peaks attributable to hotspots on the neutron star surface. The pulsed fraction evolved significantly with energy, increasing to energies around 5 keV with a steep drop thereafter. The 0.9–25 keV SXT–LAXPC energy spectrum is best described with two thermal components having temperatures ∼0.46 and ∼1.1 keV (emission radii of ∼2.4 and ∼0.65 km, respectively, assuming a distance of 4 kpc) along with a power-law component having a photon index of ∼0.39. We report the detection of 67 X-ray bursts having an average duration of ∼33 ms. The brightest burst lasted for about 90 ms and had a 3–25 keV fluence of ∼5 × 10−9 erg cm−2.
Three young planets around the K-dwarf K2-198: high-energy environment, evaporation history, and expected future
Authors: Ketzer L , Poppenhaeger K , Baratella M , Ilin E 
Published: October 2023

ABSTRACT
Planets orbiting young stars are thought to experience atmospheric evaporation as a result of the host stars’ high-magnetic activity. We study the evaporation history and expected future of the three known transiting exoplanets in the young multiplanet system K2-198. Based on spectroscopic and photometric measurements, we estimate an age of the K-dwarf host star between 200 and 500 Myr, and calculate the high-energy environment of these planets using eROSITA X-ray measurements. We find that the innermost planet K2-198c has likely lost its primordial envelope within the first few 10s of Myr regardless of the age at which the star drops out of the saturated X-ray regime. For the two outer planets, a range of initial envelope mass fractions is possible, depending on the not-yet-measured planetary mass and the stars’ spin-down history. Regarding the future of the system, we find that the outermost planet K2-198b is stable against photoevaporation for a wide range of planetary masses, while the middle planet K2-198d is only able to retain an atmosphere for a mass range between ∼7 and 18 M⊕. Lower mass planets are too susceptible to mass-loss, and a very thin present-day envelope for higher mass planets is easily lost with the estimated mass-loss rates. Our results support the idea that all three planets started out above the radius valley in the (sub-)Neptune regime and were then transformed into their current states by atmospheric evaporation, but also stress the importance of measuring planetary masses for (young) multiplanet systems before conducting more detailed photoevaporation simulations.
Clinical investigation for the mechanisms of anaphylactic symptoms in osteoarthritis patients after diclofenac etalhyaluronate administration
Authors: Nishida Yoshihiro , Yagami Akiko , Takada Shuhei , Muramatsu Dai , Nobuoka Yuji , Okayama Yoshimichi 
Published: October 2023

ABSTRACT

Objective
This study was conducted to investigate the mechanisms of anaphylaxis in patients with osteoarthritis of the knee and hip after diclofenac etalhyaluronate [product name: JOYCLU® (JCL)] intra-articular injection and to determine the utility of tests to investigate the mechanism involved.

Methods
In this observational study in Japan, patients aged ≥20 years with knee or hip osteoarthritis who received JCL intra-articular injection experienced anaphylactic symptoms considered related to JCL (‘experienced patients’) or did not experience allergic symptoms considered related to JCL (‘non-experienced patients’). Basophil activation tests (BATs), specific immunoglobulin E (IgE) antibody testing by enzyme-linked immunosorbent assays (ELISAs) or immunochromatographic kit, and genome-wide association studies (GWASs) were conducted using patient blood and saliva.

Results
Thirteen experienced patients and 14 non-experienced patients were tested. Seven experienced patients tested positive by BAT using diclofenac etalhyaluronate-containing test substances. Diclofenac-specific IgE antibodies were detected in four of seven BAT-positive patients but not in the non-experienced patients. Specific IgE antibody testing by immunochromatographic kit and genome-wide association study showed no clear results.

Conclusions
These findings suggest that anaphylaxis occurs after JCL administration via an IgE-mediated mechanism and that diclofenac etalhyaluronate may be involved in this mechanism. BAT and diclofenac -specific IgE enzyme-linked immunosorbent assay may be useful tests for investigating the mechanisms of anaphylactic reactions after JCL administration.
Diet, cellular, and systemic homeostasis control the cycling of potassium stable isotopes in endothermic vertebrates
Authors: Tacail T , Lewis J , Clauss M , Coath C D , Evershed R , Albalat E , Elliott T R , Tütken T 
Published: October 2023

AbstractThe naturally occurring stable isotopes of potassium (41K/39K, expressed as δ41K) have the potential to make significant contributions to vertebrate and human biology. The utility of K stable isotopes is, however, conditioned by the understanding of the dietary and biological factors controlling natural variability of δ41K. This paper reports a systematic study of K isotopes in extant terrestrial endothermic vertebrates. δ41K has been measured in 158 samples of tissues, biofluids, and excreta from 40 individuals of four vertebrate species (rat, guinea pig, pig and quail) reared in two controlled feeding experiments. We show that biological processing of K by endothermic vertebrates produces remarkable intra-organism δ41K variations of ca. 1.6‰. Dietary δ41K is the primary control of interindividual variability and δ41K of bodily K is +0.5–0.6‰ higher than diet. Such a trophic isotope effect is expected to propagate throughout trophic chains, opening promising use for reconstructing dietary behaviors in vertebrate ecosystems. In individuals, cellular δ41K is related to the intensity of K cycling and effectors of K homeostasis, including plasma membrane permeability and electrical potential. Renal and intestinal transepithelial transports also control fractionation of K isotopes. Using a box-modeling approach, we establish a first model of K isotope homeostasis. We predict a strong sensitivity of δ41K to variations of intracellular and renal K cycling in normal and pathological contexts. Thus, K isotopes constitute a promising tool for the study of K dyshomeostasis.
An ATP-independent role for Prp16 in promoting aberrant splicing
Authors: Chung Che-Sheng , Wai Hsu Lei , Kao Ching-Yang , Cheng Soo-Chen 
Published: October 2023

Abstract
The spliceosome is assembled through a step-wise process of binding and release of its components to and from the pre-mRNA. The remodeling process is facilitated by eight DExD/H-box RNA helicases, some of which have also been implicated in splicing fidelity control. In this study, we unveil a contrasting role for the prototypic splicing proofreader, Prp16, in promoting the utilization of aberrant 5′ splice sites and mutated branchpoints. Prp16 is not essential for the branching reaction in wild-type pre-mRNA. However, when a mutation is present at the 5′ splice site or if Cwc24 is absent, Prp16 facilitates the reaction and encourages aberrant 5′ splice site usage independently of ATP. Prp16 also promotes the utilization of mutated branchpoints while preventing the use of nearby cryptic branch sites. Our study demonstrates that Prp16 can either enhance or impede the utilization of faulty splice sites by stabilizing or destabilizing interactions with other splicing components. Thus, Prp16 exerts dual roles in 5′ splice site and branch site selection, via ATP-dependent and ATP-independent activities. Furthermore, we provide evidence that these functions of Prp16 are mediated through the step-one factor Cwc25.