bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra‐rapid lispro improved postprandial glucose control compared to insulin lispro in predominantly Chinese patients with type 1 diabetes: A prospective, randomized, double‐blind phase 3 study
Authors: Ma Jianhua , Yan Xiang , Feng Qiong , Liu Wei , Pérez Manghi Federico , García‐Hernández Pedro , Wang Guixia , Xu Jianwei , Yuan Yuan , Zhou Zhiguang 
Published: October 2023

AbstractAimsTo investigate the efficacy and safety of ultra‐rapid lispro (URLi) versus insulin lispro in predominantly Chinese patients with type 1 diabetes (T1D) in a prospective, randomized, double‐blind, treat‐to‐target, phase 3 study.Materials and MethodsFollowing a lead‐in period, during which insulin glargine U‐100 or insulin degludec U‐100 was optimized, patients were randomly assigned (1:1) to URLi (n = 176) or insulin lispro (n = 178). The primary objective was to test the noninferiority of URLi to insulin lispro in glycaemic control (noninferiority margin = 0.4% for glycated haemoglobin [HbA1c] change from baseline to week 26), with testing for the superiority of URLi to insulin lispro with regard to 1‐ and 2‐hour postprandial glucose (PPG) excursions during a mixed‐meal tolerance test and HbA1c change at week 26 as the multiplicity‐adjusted objectives.ResultsFrom baseline to week 26, HbA1c decreased by 0.21% and 0.28% with URLi and insulin lispro, respectively, with a least squares mean treatment difference of 0.07% (95% confidence interval −0.11 to 0.24; P = 0.467). URLi demonstrated smaller 1‐ and 2‐hour PPG excursions at week 26 with least squares mean treatment differences of −1.0 mmol/L (−17.8 mg/dL) and −1.4 mmol/L (−25.5 mg/dL), respectively (p < 0.005 for both) versus insulin lispro. The safety profiles of URLi and insulin lispro were similar.ConclusionsIn this study, URLi administered in a basal‐bolus regimen demonstrated superiority to insulin lispro in controlling PPG excursions, with noninferiority of HbA1c control in predominantly Chinese patients with T1D.
Application of microbial exopolysaccharides in packaging films for the food industry: a review
Authors: Guerrero Beatriz Guevara , Santos Kamila de Lima , Kamimura Eliana Setsuko , de Oliveira Carlos Augusto Fernandes 
Published: October 2023

SummaryExopolysaccharides (EPS) are high‐valued biopolymers produced by microorganisms, which are secreted to the outside of the microbial cell as capsules or soluble slime and have unique characteristics that make their application possible in multiple industries. EPS have shown antioxidant, prebiotic, and antimicrobial activity. In addition, they have been described as non‐toxic, biodegradable, and safe biopolymers for applications in food processes. Due to these characteristics, they became essential complements in the design of environmentally friendly packaging, mainly in the development of bioactive, edible, and intelligent films. In this sense, the objectives of this review were to discuss relevant issues on EPS production for environmentally sustainable food packaging, including principles, recently identified sources, and biosynthesis of different types of EPS produced by microorganisms on different substrates. The potential use of these components in manufacturing packaging films is also discussed as a promising strategy to extend the shelf life of foods.
Integrated single cell transcriptome sequencing analysis reveals species‐specific genes and molecular pathways for pig spermiogenesis
Authors: Zhao Ya‐Dan , Yang Cai‐Xia , Du Zhi‐Qiang 
Published: October 2023

AbstractMammalian spermatogenesis is a highly complicated and intricately organized process involving spermatogonia propagation (mitosis) and meiotic differentiation into mature sperm cells (spermiogenesis). In pigs, spermatogonia development and the role of somatic cells in spermatogenesis were previously investigated in detail. However, the characterization of key molecules fundamental to pig spermiogenesis remains less explored. Here we compared spermatogenesis between humans and pigs, focusing on spermiogenesis, by integrative testicular single‐cell RNA sequencing (scRNA‐seq) analysis. Human and pig testicular cells were clustered into 26 different groups, with cell‐type‐specific markers and signalling pathways. For spermiogenesis, pseudo‐time analysis classified the lineage differentiation routes for round, elongated spermatids and spermatozoa. Moreover, markers and molecular pathways specific to each type of spermatids were examined for humans and pigs, respectively. Furthermore, high‐dimensional weighted gene co‐expression network analysis (hdWGCNA) identified gene modules specific for each type of human and pig spermatids. Hub genes (pig: SNRPD2.1 related to alternative splicing; human: CATSPERZ, Ca[2+] ion channel) potentially involved in spermiogenesis were also revealed. Taken together, our integrative analysis found that human and pig spermiogeneses involve specific genes and molecular pathways and provided resources and insights for further functional investigation on spermatid maturation and male reproductive ability.
Age‐related differences in the capacity and neuromuscular control of the foot core system during quiet standing
Authors: Lai Jiaqi , Ye Yinyan , Huang Dongfeng , Zhang Xianyi 
Published: October 2023

AbstractThe foot core system is essential for upright stability. However, aging‐induced changes in the foot core function remain poorly understood. The present study aimed to examine age‐related differences in postural stability from the perspective of foot core capacity and neuromuscular control during quiet standing. Thirty‐six older and 25 young adults completed foot core capacity tests including toe flexion strength, muscle ultrasonography, and plantar cutaneous sensitivity. The center of pressure (COP) and electromyography (EMG) of abductor hallucis (ABH), peroneus longus (PL), tibialis anterior (TA) and medial gastrocnemius (GM) were simultaneously recorded during double‐leg and single‐leg standing (SLS). EMG data were used to calculate muscle synergy and intermuscular coherence across three frequency bands. Compared to young adults, older adults exhibited thinner hallucis flexors, weaker toe strength, and lower plantar cutaneous sensitivity. The ABH thickness and plantar cutaneous sensitivity were negatively associated with the COP mean peak velocity in older adults, but not in young adults. Besides, older adults had higher cocontraction of muscles spanning the arch (ABH‐PL) and ankle (TA‐GM), and had lower beta‐ and gamma‐band coherence of the ABH‐PL and TA‐PL during SLS. Foot core capacities became compromised with advancing age, and the balance control of older adults was susceptible to foot core than young adults in balance tasks. To compensate for the weakened foot core, older adults may adopt arch and ankle stiffening strategies via increasing muscle cocontraction. Furthermore, coherence analysis indicated that aging may increase the demand for cortical brain resources during SLS.
Social protection, community participation and state‐citizen relations: Evidence from a cash transfer program in south‐central Somalia
Authors: van den Boogaard Vanessa , Santoro Fabrizio , Walker Michael 
Published: October 2023

AbstractWe investigate whether social protection programs can increase participation in community‐driven development programs and examine how this affects state‐citizen relations. Using a randomized controlled trial in south‐central Somalia, we study the impacts of one‐time unconditional cash transfers to vulnerable households that were specifically designed to encourage participation in community development. While the cash transfer is relatively small as a share of annual household expenditure, it is more than sufficient to cover households' anticipated community development contributions. The transfers were funded by an NGO but delivered through state institutions. We collect survey data before and after the intervention with almost 600 individuals eligible to receive cash transfers. We find no substantial differences in participation in community development projects for cash transfer recipient households relative to non‐recipient households. However, we do find positive impacts of the cash transfers on citizen perceptions of clan elders and the local government. Our findings suggest that relatively small social protection interventions may face challenges in increasing vulnerable households' participation in community development and decision‐making, while also highlighting potential positive spillover effects for state‐citizen relations and beliefs about the capacity of local institutions where states institutions are involved in program delivery, even if they do not finance the program.
Two chromosome‐level genome assemblies of Rhodiola shed new light on genome evolution in rapid radiation and evolution of the biosynthetic pathway of salidroside
Authors: Zhang Dan‐Qing , Liu Xiao‐Ying , Qiu Lin‐Feng , Liu Zhao‐Rui , Yang Ya‐Peng , Huang Long , Wang Shi‐Yu , Zhang Jian‐Qiang 
Published: October 2023

SUMMARYRhodiola L. is a genus that has undergone rapid radiation in the mid‐Miocene and may represent a typic case of adaptive radiation. Many species of Rhodiola have also been widely used as an important adaptogen in traditional medicines for centuries. However, a lack of high‐quality chromosome‐level genomes hinders in‐depth study of its evolution and biosynthetic pathway of secondary metabolites. Here, we assembled two chromosome‐level genomes for two Rhodiola species with different chromosome number and sexual system. The assembled genome size of R. chrysanthemifolia (2n = 14; hermaphrodite) and R. kirilowii (2n = 22; dioecious) were of 402.67 and 653.62 Mb, respectively, with approximately 57.60% and 69.22% of transposable elements (TEs). The size difference between the two genomes was mostly due to proliferation of long terminal repeat‐retrotransposons (LTR‐RTs) in the R. kirilowii genome. Comparative genomic analysis revealed possible gene families responsible for high‐altitude adaptation of Rhodiola, including a homolog of plant cysteine oxidase 2 gene of Arabidopsis thaliana (AtPCO2), which is part of the core molecular reaction to hypoxia and contributes to the stability of Group VII ethylene response factors (ERF‐VII). We found extensive chromosome fusion/fission events and structural variations between the two genomes, which might have facilitated the initial rapid radiation of Rhodiola. We also identified candidate genes in the biosynthetic pathway of salidroside. Overall, our results provide important insights into genome evolution in plant rapid radiations, and possible roles of chromosome fusion/fission and structure variation played in rapid speciation.
Evaluation of the Value of the Y-Balance Test to Predict Lower Limb Injuries in Professional Male Footballers
Authors: Mohammadi Haniyeh , Ghaffari Raheleh , Kazemi Abdolreza , Bennett Hunter , Hosseinzadeh Mahdi 
Published: October 2023

Context: The aim of this prospective cohort study was to identify whether Y-Balance Test (YBT) performance and asymmetry are associated with lower limb injury in elite adult football athletes. Design: A prospective cohort study. Methods: Preseason YBT measures were obtained from 121 male footballers participating in National League One across the 2021–2022 season. Lower limb injuries were tracked across the season to determine the relationship between YBT variables and injury incidence using logistic regression analysis. The statistical significance level was .05. Results: The average YBT score was 111.0 (5.8) cm on the left limb and 112.0 (5.5) cm on the right limb, with an average asymmetry of 2.3 (1.4) cm. Athletes with lower YBT scores on both the left (odds ratio = 2.9; 95% confidence interval, 1.7 to 4.8: P ≤ .001) and right (odds ratio = 2.3; 95% confidence interval, 1.6 to 3.2: P ≤ .001) limbs were at a greater risk of injury. Similarly, athletes with greater amounts of asymmetry were also more likely to get injured (odds ratio = 2.1; 95% confidence interval, 1.3 to 3.3: P = .002). Conclusions: Results indicate that lower and asymmetrical YBT scores have a significant relationship with future lower limb injuries in professional male footballers. The YBT offers a simple, reliable, and effective screening tool that can be used by practitioners in football to help identify players at a greater risk of injury before the season commences.
In vitro
reconstitution of herpes simplex virus 1 fusion identifies low pH as a fusion co-trigger
Authors: Ramirez J. Martin , Calderon-Zavala Ariana C. , Balaram Ariane , Heldwein Ekaterina E. 
Published: October 2023

ABSTRACT

Membrane fusion mediated by herpes simplex virus 1 (HSV-1) is a complex, multi-protein process that is receptor triggered and can occur both at the cell surface and in endosomes. To deconvolute this complexity, we reconstituted HSV-1 fusion with synthetic lipid vesicles
in vitro
. Using this simplified, controllable system, we discovered that HSV-1 fusion required not only a cognate host receptor but also low pH. On the target membrane side, efficient fusion required cholesterol, negatively charged lipids found in the endosomal membranes, and an optimal balance of lipid order and disorder. On the virion side, the four HSV-1 entry glycoproteins—gB, gD, gH, and gL—were sufficient for fusion. We propose that low pH is a biologically relevant co-trigger for HSV-1 fusion. The dependence of fusion on low pH and endosomal lipids could explain why HSV-1 enters most cell types by endocytosis. We hypothesize that under neutral pH conditions, other, yet undefined, cellular factors may serve as fusion co-triggers. The
in vitro
fusion system established here can be employed to systematically investigate HSV-1-mediated membrane fusion.

IMPORTANCE

HSV-1 causes lifelong, incurable infections and diseases ranging from mucocutaneous lesions to fatal encephalitis. Fusion of viral and host membranes is a critical step in HSV-1 infection of target cells that requires multiple factors on both the viral and host sides. Due to this complexity, many fundamental questions remain unanswered, such as the identity of the viral and host factors that are necessary and sufficient for HSV-1-mediated membrane fusion and the nature of the fusion trigger. Here, we developed a simplified
in vitro
fusion assay to examine the fusion requirements and identified low pH as a co-trigger for virus-mediated fusion
in vitro
. We hypothesize that low pH has a critical role in cell entry and, potentially, pathogenesis.
Chlamydial YAP activation in host endocervical epithelial cells mediates pro-fibrotic paracrine stimulation of fibroblasts
Authors: Caven Liam , Carabeo Rey 
Published: October 2023

ABSTRACT

Infection of the female genital tract by
Chlamydia trachomatis
can produce severe fibrotic sequelae, including tubal factor infertility and ectopic pregnancy. While infection demonstrably mediates a pro-fibrotic response in host cells, it remains unclear if intrinsic properties of the upper genital tract exacerbate chlamydial fibrosis. The relatively sterile environment of the upper genital tract is primed for a pro-inflammatory response to infection, potentially enhancing fibrosis; however, subclinical
C. trachomatis
infections still develop fibrosis-related sequelae. Here, we compare infection-associated and steady-state gene expression of primary human cervical and vaginal epithelial cells. In the former, we observe enhanced baseline expression and infection-mediated induction of fibrosis-associated signal factors (e.g.,
TGFA
,
IL6
,
IL8
, and
IL20
), implying predisposition to
Chlamydia
-associated pro-fibrotic signaling. Transcription factor enrichment analysis identified regulatory targets of YAP, a transcriptional cofactor induced by infection of cervical epithelial cells, but not vaginal epithelial cells. YAP target genes induced by infection include secreted fibroblast-activating signal factors; therefore, we developed an
in vitro
model involving coculture of infected endocervical epithelial cells with uninfected fibroblasts. Coculture enhanced fibroblast expression of type I collagen, as well as prompting reproducible (albeit statistically insignificant) induction of α-smooth muscle actin. Fibroblast collagen induction was sensitive to siRNA-mediated YAP knockdown in infected epithelial cells, implicating chlamydial YAP activation in this effect. Collectively, our results present a novel mechanism of fibrosis initiated by
Chlamydia
, wherein infection-mediated induction of host YAP facilitates pro-fibrotic intercellular communication. Chlamydial YAP activation in cervical epithelial cells is thus a determinant of this tissue’s susceptibility to fibrosis.

IMPORTANCE

Chronic or repeated infection of the female upper genital tract by
C. trachomatis
can lead to severe fibrotic sequelae, including tubal factor infertility and ectopic pregnancy. However, the molecular mechanisms underlying this effect are unclear. In this report, we define a transcriptional program specific to
C. trachomatis
infection of the upper genital tract, identifying tissue-specific induction of host YAP—a pro-fibrotic transcriptional cofactor—as a potential driver of infection-mediated fibrotic gene expression. Furthermore, we show that infected endocervical epithelial cells stimulate collagen production by fibroblasts and implicate chlamydial induction of YAP in this effect. Our results define a mechanism by which infection mediates tissue-level fibrotic pathology via paracrine signaling and identify YAP as a potential therapeutic target for the prevention of
Chlamydia
-associated scarring of the female genital tract.
Use of a Woven Endobridge device (WEB) for the treatment of an aneurysmal diverticulum of the transverse sinus causing pulsatile tinnitus
Authors: Fageeh Areej , Zur Gil , Diouf Ange , Drake Brian , P dos Santos Marlise , Lui Anne , Karwaski Stephen , Chenier Chad , Trussler Laurie , Barnes Nancy , Tse Darren , Schramm David , Lesiuk Howard , Fahed Robert 
Published: October 2023

Pulsatile tinnitus is a symptom with a potentially significant impact on the quality of life of patients.1 In some cases the pulsatile tinnitus is secondary to an arterial, arteriovenous, or a venous condition that can be treated endovascularly.2–5 One of the newly recognized entities that can cause pulsatile tinnitus is the presence of an ipsilateral aneurysmal diverticulum of the transverse sinus. The Woven EndoBridge (WEB) is an intra-aneurysmal flow disruptor for the treatment of broad-based arterial aneurysms with a high safety and effectiveness profile.3 The initial version of the WEB with a dual-layer structure evolved into a single-layer structure in two different versions (WEB SL, a barrel shape, and WEB SLS, a spherical shape).4 The WEB system does not require concomitant antiplatelet therapy, unlike other intraluminal devices such as flow diverters or intracranial stents. We describe a case of pulsatile tinnitus secondary to an aneurysmal diverticulum of the transverse sinus successfully treated with a WEB SL device instead of stent-assisted coiling, therefore alleviating the need for antiplatelets (video 1). The patient had an immediate clinical response with complete and persisting disappearance of her pulsatile tinnitus.Video 1-
Effects of odderon spin on helicity amplitudes in pp elastic scattering
Authors: Sawasdipol Prin , Magallanes Jingle B. , Pongkitivanichkul Chakrit , Samart Daris 
Published: October 2023

AbstractIn recent years, the discovery of the odderon, a colorless C-odd gluonic compound, has been confirmed in the TOTEM and D0 collaborations. However, the spin quantum number of the odderon remains unidentified. In this work, we aim to attribute a spin of $$J=3$$

J
=
3

to the odderon in pp elastic scattering by calculating the helicity amplitudes and the corresponding complex parameter $$r_5$$

r
5

, the ratio of helicity’s single-flip to non-flip amplitudes, for the spin-3 tensor odderon with the standard spin-2 tensor pomeron exchanges. Then, we apply these results to the constraints obtained from the STAR experiment at RHIC. By comparing to the contributions of the spin-1 vector odderon and spin-2 tensor pomeron, we demonstrate that the spin-3 tensor odderon, i.e. $$J=3$$

J
=
3

, provides a better explanation for the observable in pp elastic scattering.
Effects of Mulberry Leaf Extract on the Liver Function of Juvenile Spotted Sea Bass (Lateolabrax maculatus)
Authors: Zhou Sishun , Lin Hao , Kong Lumin , Ma Jianrong , Long Zhongying , Qin Huihui , Huang Zhangfan , Lin Yi , Liu Longhui , Li Zhongbao 
Published: October 2023

In order to explore the effect of mulberry leaf extract (ELM) on the liver function of spotted sea bass, 360 fish with healthy constitution (average body weight 9.00 ± 0.02 g) were selected and randomly divided into six groups with three repetitions, and six groups of fish were randomly placed into 18 test tanks (200 L) with 20 fish per tank for the 52-day feeding test. Every day, the fish were fed the experimental feed with different concentrations (0, 3, 6, 9, 12, 15 g/kg) to the level of apparent satiation, with a crude protein content of 48.0% and a crude fat content of 8.6%. And the water temperature was maintained at 25–28°C with a salinity of 0.5%–1‰. After feeding, five fish were randomly selected to collect their livers and serum for detection of indicators. The results showed that, compared with the control group, ELM significantly increased the activities of lipase (LPS) and trypsin (TRS) in the liver, and reached the highest level when the amount of ELM added was 6 g/kg

P
<
0.05

. ELM significantly increased the activities of lactate dehydrogenase (LDH) and glutamic-oxaloacetic transaminase (GOT) involved in the metabolic process in liver tissue, and GOT activity reached the highest when ELM was added at 9 g/kg, and LDH activity reached the highest when ELM was added at 15 g/kg

P
<
0.05

. ELM had no significant effect on liver antioxidant enzymes

P
>
0.05

, but the content of malondialdehyde was significantly reduced

P
<
0.05

. Compared with the control group, ELM significantly increased the activities of AKP and ACP in the liver, and the AKP activity reached the highest when the ELM addition amount was 3 g/kg, and the ACP activity reached the highest when the ELM addition amount was 9 g/kg

P
<
0.05

. Through comparative transcriptomic analysis, it was indicated that ELM enhanced the hepatic lipids and carbohydrates metabolism ability, as manifested in the upregulation of expression of phosphatidate phosphatase, glucuronosyltransferase, inositol oxygenase, carbonic anhydrase, and cytochrome c oxidase subunit 2. ELM can also increase the expression of signal transducer and activator of transcription 1, ATP-dependent RNA helicase and C-X-C motif chemokine 9 involved in the immune process. The above results show that the ELM can enhance the digestion, metabolism, and immunity of the liver by increasing the activity of digestive enzymes, metabolic enzymes, and the expression of metabolism and immune regulation genes. This study provides a theoretical basis for the application of ELM in the cultivation of spotted sea bass by exploring the effect of ELM on the liver function of spotted sea bass.