bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immigration background as a risk factor of chronic pain and high-impact chronic pain in children and adolescents living in Spain: differences as a function of age
Authors: Roman-Juan Josep , Sánchez-Rodríguez Elisabet , Solé Ester , Castarlenas Elena , Jensen Mark P. , Miró Jordi 
Published: January 2024

Abstract
The number of people immigrating from one country to another is increasing worldwide. Research has shown that immigration background is associated with chronic pain and pain disability in adults. However, research in this issue in children and adolescents has yielded inconsistent results. The aims of this study were to examine (1) the association between immigration background and chronic pain in children and adolescents; (2) the association between immigration background and pain interference in children and adolescents with chronic pain; and (3) the extent these associations differed as a function of sex and age. Participants of this cross-sectional study were 1115 school children and adolescents (mean age = 11.67 years; 56% girls). Participants were asked to provide sociodemographic information and respond to a survey including measures of pain (location, extension, frequency, intensity, and interference). Results showed that having an immigration background was associated with a greater prevalence of chronic pain (odds ratio [OR] = 1.91, P < 0.001) and that this association was higher in children (OR = 6.92, P < 0.001) and younger adolescents (OR = 1.66, P < 0.05) than in older adolescents. No significant association between immigration background and pain interference was identified. Children and adolescents with an immigration background, especially younger children, are at higher risk for having chronic pain. More resources should be allocated in the prevention of chronic pain in children and adolescents with an immigration background.
Description and initial validation of a novel measure of pain intensity: the Numeric Rating Scale of Underlying Pain without concurrent Analgesic use
Authors: Suri Pradeep , Heagerty Patrick J. , Timmons Andrew , Jensen Mark P. 
Published: January 2024

Abstract
Although many individuals with chronic pain use analgesics, the methods used in many randomized controlled trials (RCTs) do not sufficiently account for confounding by differential post-randomization analgesic use. This may lead to underestimation of average treatment effects and diminished power. We introduce (1) a new measure—the Numeric Rating Scale of Underlying Pain without concurrent Analgesic use (NRS-UP(A))—which can shift the estimand of interest in an RCT to target effects of a treatment on pain intensity in the hypothetical situation where analgesic use was not occurring at the time of outcome assessment; and (2) a new pain construct—an individuals' perceived effect of analgesic use on pain intensity (EA). The NRS-UP(A) may be used as a secondary outcome in RCTs of point treatments or nonpharmacologic treatments. Among 662 adults with back pain in primary care, participants' mean value of the NRS-UP(A) among those using analgesics was 1.2 NRS points higher than their value on the conventional pain intensity NRS, reflecting a mean EA value of −1.2 NRS points and a perceived beneficial effect of analgesics. More negative values of EA (ie, greater perceived benefit) were associated with a greater number of analgesics used but not with pain intensity, analgesic type, or opioid dose. The NRS-UP(A) and EA were significantly associated with future analgesic use 6 months later, but the conventional pain NRS was not. Future research is needed to determine whether the NRS-UP(A), used as a secondary outcome may allow pain RCTs to target alternative estimands with clinical relevance.
Nutritional deficiencies in children
Authors: Ali Farhana , Mouzaki Marialena 
Published: January 2024

Purpose of review
The purpose of this review is to summarize commonly encountered nutritional deficiencies in children and their implications. Considering data suggesting that the majority of children with the United States consume unhealthy diets, the growing interest in the consumption of limiting diets, as well as the insidious clinical presentation of nutritional deficiencies, this is a timely and highly relevant review.

Recent findings
The underlying socioeconomic and medical circumstances that predispose to nutritional deficiencies in the Western world are covered. The high index of suspicion required to recognize nutritional deficiencies and the limitations of available testing are also discussed. Finally, the need for the development of accurate nutritional biomarkers is presented as a future research priority.

Summary
Nutritional deficiencies are not uncommon, even in high resource countries. Clinicians should remain vigilant and include nutritional deficiencies in the differential diagnoses of patients presenting with nonspecific symptoms.
Increased extra-axial cerebrospinal fluid and benign external hydrocephalus in early autism
Authors: Ben-Arie Gal , Shelef Ilan , Meiri Gal , Menashe Idan , Dinstein Ilan , Arazi Ayelet 
Published: January 2024

AbstractPrevious studies have reported that some children with Autism Spectrum Disorder (ASD) below the age of 3-years-old exhibit increased extra-axial cerebrospinal fluid (EA-CSF) volumes, suggesting that transient early CSF circulation abnormalities may characterize a unique etiology in a sub-group of ASD children. Specifically, this sub-group was proposed to exhibit an EA-CSF to total brain volume (TBV) ratio >0.14. It is unknown whether such findings may correspond to an early radiological diagnosis of Benign external hydrocephalus (BEH), which is defined as an enlargement of CSF volumes within the subarachnoid space (SAS) during the first two years of life.Here we examined a retrospective clinical sample extracted from the National Autism Database of Israel. In 2020, ∼14% of ASD children in the database (n= 102) had completed clinical brain MRI scans at different ages before or after ASD diagnosis. Scans of 83 ASD children fulfilled quality control criteria and were compared to those of 53 age and sex matched typicality developing (TD) children who were referred to the same clinical center for similar clinical reasons. EA-CSF volume and total brain volume (TBV) were quantified from T2-weighted scans using manual labeling and compared across groups.The results revealed that ASD children scanned before the age of 2-years-old (i.e., before ASD diagnosis) exhibited significantly larger EA-CSF volumes when compared to controls (t(49)=2.89, p=0.006, Cohen’s d=0.82). No significant differences were apparent across groups at older ages. Of those scanned before the age of 2-years-old, ∼16% of the ASD children, and none of the control children, exhibited EA-CSF/TBV ratios >0.14, thereby replicating previous findings from prospective research samples. In addition, retrospective examination of the neuroradiology assessments revealed a BEH prevalence of 33% in the ASD children scanned before the age of 2-years-old, with 80% of these children exhibiting EA-CSF/TBV ratios >0.11.These findings reveal a clear relationship between quantitative EA-CSF volume analyses and qualitative BEH radiological findings, which characterize a considerable sub-group of ASD children. Accumulating evidence, therefore, suggests that BEH diagnosis in conjunction with a high EA-CSF/TBV ratio by the age of 2-years-old may be indicative of a specific ASD etiology that warrants further research and may offer unique opportunities for intervention.
Deletion of absent in melanoma (AIM) 2 gene alters bone morphology
Authors: Gong Zhenwei , Dixit Manisha , Poudel Sher Bahadur , Yildirim Gozde , Yakar Shoshanna , Muzumdar Radhika 
Published: January 2024

ABSTRACTAbsent in Melanoma (AIM) 2 is a gene that is induced by interferon and acts as a cytosolic sensor for double-stranded (ds) DNA. It forms the AIM2 inflammasome, leading to the production of interleukin (IL)-1β and IL-18. Our previous research demonstrated that mice lacking AIM2 exhibit spontaneous obesity, insulin resistance, and inflammation in adipose tissue. In this study, we aimed to explore the impact of AIM2 gene deletion on bone structure in adult and aged mice. Utilizing micro-computed tomography (micro-CT), we discovered that female mice lacking AIM2 showed an increase in the total cross-sectional area at 5 months of age, accompanied by an increase in cortical thickness in the mid-diaphysis of the femur at both 5 and 15 months of age. At 15 months of age, the cortical bone mineral density (BMD) significantly decreased in AIM2 null females compared to wild-type (WT) mice. In AIM2 null mice, both trabecular bone volume and BMD at the distal metaphysis of the femur significantly decreased at 5 and 15 months of age. Similarly, micro-CT analysis of the L4 vertebra revealed significant decreases in trabecular bone volume and BMD in aged AIM2 null females compared to WT mice. Histological examination of femurs from aged mice demonstrated increased bone marrow adiposity in AIM2 null mice, accompanied by a significant increase in CD45-/CD31-/Sca1+/Pdgfa+ adipose progenitor cells, and a decrease in the ratio of CD31-/CD31+ osteogenic progenitor cells, as determined by flow cytometry of bone marrow cells. Our findings suggest that AIM2 deficiency affects bone health by promoting adipogenesis in bone marrow cells and inducing a pro-inflammatory environment, potentially contributing to the decreased bone mineral density.
Heparan sulfate selectively inhibits the collagenase activity of cathepsin K
Authors: Zhang Xiaoxiao , Luo Yin , Hao Huanmeng , Krahn Juno M. , Su Guowei , Dutcher Robert , Xu Yongmei , Liu Jian , Pedersen Lars C. , Xu Ding 
Published: January 2024

AbstractCathepsin K (CtsK) is a cysteine protease with potent collagenase activity. CtsK is highly expressed by bone-resorbing osteoclasts and plays an essential role in bone remodeling. Although CtsK is known to bind heparan sulfate (HS), the structural details of the interaction, and how HS ultimately regulates the biological functions of CtsK, remains largely unknown. In this report, we determined that CtsK preferably binds to larger HS oligosaccharides, such as dodecasaccharides (12mer), and that the12mer can induce monomeric CtsK to form a stable dimer in solution. Interestingly, while HS has no effect on the peptidase activity of CtsK, it greatly inhibits the collagenase activity of CtsK in a manner dependent on sulfation level. By forming a complex with CtsK, HS was able to preserve the full peptidase activity of CtsK for prolonged periods, likely by stabilizing its active conformation. Crystal structures of Ctsk with a bound 12mer, alone and in the presence of the endogenous inhibitor cystatin-C reveal the location of HS binding is remote from the active site. Mutagenesis based on these complex structures identified 6 basic residues of Ctsk that play essential roles in mediating HS-binding. At last, we show that HS 12mers can effectively block osteoclast resorption of bonein vitro. Combined, we have shown that HS can function as a multifaceted regulator of CtsK and that HS-based oligosaccharide might be explored as a new class of selective CtsK inhibitor in many diseases that involve exaggerated bone resorption.
Intestinal flow rates, absorption of felodipine from the small intestine and attributes of chyme collected at midgut from Labradors
Authors: Diebold Steffen M. ,   In memory of Mia Wilsson-Rahmberg
Published: January 2024

AbstractThe objectives of the present study were (1) to investigate gastrointestinal hydrodynamics of Labradors as a model for human midgut (2) to examine various attributes of intestinal fluids in vivo and (3) to study the influence of hydrodynamics on the dissolution and absorption of a poorly soluble drug from various suspensions.Gastrointestinal flow rates were determined volumetrically using an aspiration method. Isotonic saline and 20 % glucose solutions were used to alter gastrointestinal hydrodynamics. Felodipine, a BCS class II substance, was suspended in these fluids. Osmolality, pH, bile acid concentration and drug solubility in various chyme samples were determined. Blood plasma levels of felodipine were recorded while gastrointestinal dissolution was ongoing.Fluid recovery at midgut fistula was significantly higher (>100 %) for glucose 20 % than for isotonic saline solutions (70 %). After administration of 200 ml glucose 20 % the (overall) grand median of differential gastrointestinal flow rates (DFR) was 8.3 ml/min.. Individual spike flow ranged from 20 up to 60 ml/min. Corresponding flow rates after administration of 200 ml isotonic saline were 35.0 ml/min. for the grand median including individual spike flows beyond 100 ml/min.. Within and between-dog variability in flow rate data was similar. In general, glucose solutions released more evenly. Following oral administration of glucose solution 20 % osmolality of intestinal fluids decreased within 40 min. from about 1000 mOsm. towards more physiological values of about 350 mOsm.. Saturation solubility of felodipine (Cs) in jejunal chyme after administration of either solution (saline or glucose) was determined to be about 10 (µg/ml) on average (median),exposing high variability with time! The intestinal solubility varied greatly within the course of an experiment. However, a strong correlation was observed between the aspirated fluid volume and the dissolved amount of felodipine confirming the well known relationship of Noyes, Whitney, Nernst and Brunner in-vivo. Grand median of pH in jejunal chyme of labradors was determined to be 6.68. Median values range from 4.38-7.62. The pharmacokinetic data showed a slight trend to differences based on particle size and on fluid administered.
Reprogramming of 3D genome structure underlying HSPC development in zebrafish
Authors: He Min , Li Xiaoli , Xu Bingxiang , Lu Yibo , Liu Huakai , An Ziyang , Zhang Wenqing , Li Feifei 
Published: January 2024

AbstractDevelopment of hematopoietic stem and progenitor cells (HSPC) is a multi-staged complex process that conserved between zebrafish and mammals; however, the mechanism underlying HSPC development is not fully understood. Chromatin conformation plays important roles in transcriptional regulation and cell fate decision, its dynamic and role in HSPC development is poorly investigated. Here, we performed chromatin structure and multi-omics dissection across different stages of HSPC developmental trajectory in zebrafish. Chromatin organization of zebrafish HSPC resemble mammalian cells with similar hierarchical structure and characteristics. We revealed the multi-scale reorganization of 3D genome and its influence on transcriptional regulation and transition of cell function during HSPC development. Nascent HSPC is featured by loose conformation with obscure structure at all layers. Notably, PU.1 was identified as a potential factor mediating formation of promoter-involved loops and regulating gene expression as well as HSPC function. Our results provided a global view of chromatin structure dynamics associated with development of zebrafish HSPC and discovered key transcription factor involved in HSPC chromatin interactions, which will provide new insights into the epigenetic regulatory mechanisms underlying vertebrate HSPC fate decision.
Cytochrome P450 Enzyme Design by Constraining Catalytic Pocket in Diffusion model
Authors: Wang Qian , Liu Xiaonan , Zhang Hejian , Chu Huanyu , Shi Chao , Zhang Lei , Liu Pi , Li Jing , Zhu Xiaoxi , Liu Yuwan , Chen Zhangxin , Huang Rong , Bai Jie , Chang Hong , Liu Tian , Chang Zhenzhan , Cheng Jian , Jiang Huifeng 
Published: January 2024

AbstractAlthough cytochrome P450 enzymes are the most versatile biocatalysts in nature, there is insufficient comprehension of the molecular mechanism underlying their functional innovation process. Here, by combining ancestral sequence reconstruction, reverse mutation assay and structure analysis, we identified five founder residues in the catalytic pocket of flavone 6-hydroxylase (F6H) and proposed a “three-point fixation” model to elucidate the functional innovation mechanisms of P450s in nature. According to this design principle of catalytic pocket, we further developed a de novo diffusion model (P450Diffusion) to generate artificial P450s. Ultimately, among the 17 non-natural P450s we generated, ten designs exhibited significant F6H activity and six exhibited a 1.3- to 3.5-fold increase in catalytic capacity compared to the natural CYP706X1. This work not only explores the design principle of catalytic pockets of P450s, but also provides an insight into the artificial design of P450 enzymes with desired functions.
Butterfly-themed collectible African phonecards
Authors: Tavares Wagner De Souza , Silitonga Rani Uli 
Published: January 2024

Purpose
Phonecards were largely used in African countries for telephone services. They have been collected by people around the world as a hobby. Images from several themes were printed in phonecards, including insects (beetles, butterflies, dragonflies, etc.). We hypothesized that the display of butterfly images in phonecards aimed to arise issues associated with butterfly endangerment and conservation status by protecting their natural habitat and/or butterfly beautifulness, and that they present “extinct” or “threatened” conservation status classification. The purpose of this study is to describe how butterfly images were used in African phonecards; to identify the butterfly species name through their images and information available in all African phonecards; and to record the most recent known geographical distribution and conservation status of butterflies.

Design/methodology/approach
Colnect database was assessed to identify all butterfly-themed African phonecards. International Union for Conservation of Nature database was also assessed to obtain information on the most recent known geographical distribution and conservation status of butterflies.

Findings
The species name of butterflies was identified from 6 out of 18 African phonecards. The most recent known geographical distribution of butterflies was wide across Africa and conservation status of most genera is classified as “least concern”.

Originality/value
The display of butterfly images in African phonecards is mostly associated with their beautifulness and least related with butterfly conservation, protection and endangerment status.
Unleashing innovation and agility: interaction between intellectual capital and supply chain analytics
Authors: Al-Omoush Khaled Saleh , Ribeiro-Navarrete Samuel , Palomo Maite , Jaspe Nieto Javier 
Published: January 2024

PurposeThis study explores the impact of intellectual capital on the adoption of supply chain analysis by manufacturing companies. The authors also examine the potential role of supply chain analytics in supply chain innovation and agility.Design/methodology/approachData were gathered from 268 managers and directors of Jordanian companies. The hypotheses were tested using the Smart PLS software.FindingsThe results reveal that human, structural and social capital significantly impact supply chain analytics. Moreover, the findings show that supply chain analytics significantly affect supply chain innovation and agility. In other words, cultivating intellectual capital is crucial for utilizing supply chain analysis to enhance performance in terms of innovation and agility.Originality/valueThis study adds to the literature on the determinants of the adoption of supply chain analytics and its function in establishing the dynamic capabilities of businesses, including supply chain innovation and agility.
Indigenous healing practices in Bangladesh: does science think parallel to these or not?
Authors: Yesmin Shamima , Akhter Ayesha 
Published: January 2024

Purpose
A shared set of moral standards, ethical principles and behavioral norms of social structure can be referred to as culture. Many health problems are strongly influenced by one’s cultural background. The purpose of the paper is to examine the scientific explanation of indigenous norms and practice of health healing.

Design/methodology/approach
This qualitative study considered in-person interviews to know the Tribals’ indigenous healing practice in Bangladesh. A focus group discussion with five tribal students was conducted to form a baseline on Tribals’ norms, rituals and information-sharing behavior. Around 35 tribal students were interviewed to find out their healing practices, norms and rituals on health issues. All these practicing indigenous knowledge were documented instantly. Peer-reviewed scientific papers from renowned databases were searched to have scientific evidence on each case. All the studies having negative or positive evidence were mentioned with each case.

Findings
The findings showed more indigenous knowledge with scientific disagreements on health aspects among the Tribals’ health practice in Bangladesh. However, the positive impact of such knowledge is not negligible. Therefore, showcasing the scientific tribals’ indigenous knowledge to a global audience is a strong recommendation.

Originality/value
Health and health care-seeking behavior among the tribal population in Bangladesh is not a new area of research, few studies have focused on the context, reasons and choices in patterns of health care-seeking behavior; obstacles and challenges faced in accessing health-care provision in the tribal areas in the country. However, research attempts to show the relationship between ecological knowledge and scientific indication is new in nature.
Towards the measurement of stereotype threat: scale development and validation
Authors: Sultana Almaas , Farooq Rayees 
Published: January 2024

Purpose
The purpose of the study is to develop a valid measure of stereotype threat.

Design/methodology/approach
A convenience sample of 2,900 respondents from different occupational sectors, including managers, engineers and health-care professionals, was used for the present study. The data were collected from various government and private organizations in North India. The questionnaire survey was administered in three phases. During the first phase, 800 questionnaires were circulated, followed by 1,200 questionnaires in the second phase, and the third phase involves 900 questionnaires. The data were analysed using exploratory factor analysis, confirmatory factor analysis and structural equation modelling.

Findings
The results indicate nine dimensions, namely, occupational identification, occupational stigma consciousness, gender identification, gender stigma consciousness, religion identification, religion stigma consciousness, caste identification, caste stigma consciousness and negative effect of stereotype threat. The study ensures the reliability and validity of the stereotype threat scale. The measure also fulfils the assumptions of nomological validity.

Originality/value
To the best of the authors’ knowledge, the study is the first of its kind to develop and validate the stereotype threat scale adhering to scale development procedures.