bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of the transcription factor CdnL promotes adaptation to nutrient stress inCaulobacter
Authors: Smith Erika L. , Panis Gaël , Woldemeskel Selamawit Abi , Viollier Patrick H. , Chien Peter , Goley Erin D. 
Published: December 2023

ABSTRACTIn response to nutrient deprivation, bacteria activate a conserved stress response pathway called the stringent response (SR). During SR activation inCaulobacter crescentus, SpoT synthesizes the secondary messengers (p)ppGpp, which affect transcription by binding RNA polymerase to downregulate anabolic genes. (p)ppGpp also impacts expression of anabolic genes by controlling the levels and activities of their transcriptional regulators. InCaulobacter, a major regulator of anabolic genes is the transcription factor CdnL. If and how CdnL is controlled during the SR and why that might be functionally important is unclear. Here, we show that CdnL is downregulated post-translationally during starvation in a manner dependent on SpoT and the ClpXP protease. Inappropriate stabilization of CdnL during starvation causes misregulation of ribosomal and metabolic genes. Functionally, we demonstrate that the combined action of SR transcriptional regulators and CdnL clearance allows for rapid adaptation to nutrient repletion. Moreover, cells that are unable to clear CdnL during starvation are outcompeted by wild-type cells when subjected to nutrient fluctuations. We hypothesize that clearance of CdnL during the SR, in conjunction with direct binding of (p)ppGpp and DksA to RNAP, is critical for altering the transcriptome in order to permit cell survival during nutrient stress.SIGNIFICANCEThe stringent response (SR) is a ubiquitous bacterial stress response that promotes adaptation to nutrient deprivation. While it is known that SR activation affects RNA polymerase activity to reprogram the transcriptome, the impact of the SR on other transcriptional regulators is not well understood. Here, we show that a conserved transcription factor, CdnL, is cleared upon activation of the SR, and that its clearance is important for cells to efficiently adapt to nutrient fluctuations. Our results suggest that CdnL regulation enables adaptation by transcriptionally downregulating ribosome biosynthesis and flux through metabolic pathways, thereby promoting survival during nutrient stress. As CdnL homologs are broadly found, we hypothesize that CdnL regulation is a conserved mechanism of bacterial adaptation to stress.
Advancing Reef Monitoring Techniques through Exometabolomics: Quantification of Labile Dissolved Organic Metabolites on Coral Reefs
Authors: Garcia Brianna M. , Becker Cynthia C. , Weber Laura , Swarr Gretchen J. , Kido Soule Melissa C. , Apprill Amy , Kujawinski Elizabeth B. 
Published: December 2023

AbstractExtracellular chemical cues constitute much of the language of life among marine organisms from microbes to mammals. These chemical cues shape foraging and feeding strategies, symbiotic interactions, selection of mates and habitats, and the transfer of energy and nutrients within and among ecosystems. Changes in the chemical pool flux within these ecosystems serve as invisible signals of overall ecosystem health and, conversely, disruption to this finely tuned equilibrium. In coral reef systems, the full scope and magnitude of the chemicals involved in maintaining reef equilibria are largely unknown. In particular, processes involving small, polar molecules, which form the majority components of labile dissolved organic carbon (DOC), are often poorly captured using traditional techniques and therefore remain unconstrained. In this study, we employed a recently developed chemical derivatization method and mass spectrometry-based targeted exometabolomics to capture and quantify polar dissolved phase metabolites on five coral reefs in the U.S. Virgin Islands. We detected and quantified 45 polar exometabolites, and further demonstrate their variability across the investigated geographic landscape and contextualize these findings in terms of geographic and benthic cover differences. By comparing our results to previously published coral reef exometabolomes that did not employ chemical derivatization, we show the novel quantification of 23 metabolites which were previously undetected in the coral reef exometabolome, including compounds involved in central carbon metabolism (e.g.,glutamate) and novel metabolites such as homoserine betaine. We highlight the immense potential of chemical derivatization-based exometabolomics for quantifying labile chemical cues on coral reefs and measuring molecular level responses to environmental stressors. Overall, improving our understanding of the composition and dynamics of reef exometabolites is vital for effective ecosystem monitoring and management strategies.SynopsisMinimal research exists on the polar exometabolites on coral reefs, an essential component of reef health and the majority component of labile DOC. This study reports 23 newly quantified polar metabolites on coral reefs and highlights significant differences in the exometabolome composition across geographically distinct reefs and bays.
The first chromosome-level annotated reference genome forChrosomus eos, the northern redbelly dace
Authors: Schultz Ben , Mandeville Elizabeth G. 
Published: December 2023

AbstractHybridization amongst teleost fish species is an ongoing phenomenon with unclear implications.Chrosomus eos, the northern redbelly dace, is a species with an especially complex and fascinating history of hybridization with the closely related speciesChrosomous neogaeus, the finescale dace. The two species historically hybridized during the last glacial maximum, forming a rare unisexual F1 hybrid lineage that utilizes gynogenetic reproduction. This hybridization event is currently thought to have only occurred during the glacial period, with subsequent dispersal of parents and hybrids into contemporary ranges during glacial recession and a cessation of further hybridization. All three maintain sympatric relationships, with limited evidence suggesting recent or contemporary hybridization between the parental species. To enable future research, a novel, high-quality, chromosome-level genome was produced through Dovetail Genomics and analyzed for completeness and the syntenic relationship between itself and the high-quality, well cited zebrafishDanio reriogenome.
Revitalisation of urban spaces by women architects: enhancing cultural heritage in the gulf region
Authors: Moscatelli Monica , Raffa Alessandro , Ulusoy Shipstone Arzu 
Published: December 2023

Purpose This study aims to demonstrate how women's involvement in urban planning and design in Gulf cities improves urban space's inclusivity and strengthens identity through cultural heritage revitalisation. It also promotes the participation of women in architecture and city-making by showcasing how shaping urban spaces offers local communities opportunities for social interaction and a more inclusive environment.Design/methodology/approach The paper critically compares two case studies in the Gulf region—one in the United Arab Emirates and the other in Bahrain—according to four inclusion criteria: context connection, cultural sensitivity, community engagement and choices of amenities. These inclusion criteria are also applied to an experimental project by women architects' students in Saudi Arabia to inspire the future female architects of the Gulf region. From urban to architectural scales, the project offers a glance into the heritage design by women architects.Findings In light of this critical analysis, this study highlights the sensitivity to issues related to the revitalisation of urban areas by women architects. The case studies identified show the role of the female architect in making architecture and linking cultural heritage with contemporary themes. These projects stitch the past with the present and link cultural identity with aspects related to sustainable architecture. Therefore, valorising women's architectural experience is necessary to contribute to sustainable urban development in the Gulf region and beyond.Originality/value The present study addresses the importance of the role of women architects in the Gulf region. The research promotes the full and equal participation of women in the architecture and construction of the city to recognise their achievements by increasing their involvement in the work in a more integrated and balanced way.
An eye-tracking-based approach to evaluate the usability of government portal websites in pilot smart cities
Authors: Li Dezhi , Zhou Huan , Zhou Shenghua , Huang Guanying , Ma Xiaoming , Zhao Yongheng , Wang Wentao , Ng S. Thomas 
Published: December 2023

PurposeThe study aims to pioneer an innovative approach for the evaluation of government portal websites (GPWs) by introducing an eye-tracking-based method. The research meticulously pinpoints and analyses the distinct usability issues and challenges that users encounter while navigating and interacting with GPWs.Design/methodology/approachThis study devises an eye-tracking-based GPW usability evaluation approach, which focuses on the major functions (i.e. government information disclosure, government services and interactive responses) of GPWs. An Entropy Weighted Technique for Order Preference by Similarity to an Ideal Solution (EW-TOPSIS) method is employed to process eye-tracking indicator results for deriving GPW usability results.FindingsThe proposed approach is demonstrated to assess the usability of 12 GPWs in pilot smart cities in China, and it is found that most GPWs have lower-than-average usability. GPWs with low usability require more cognitive load that exhibit increased fixation and saccade. The comparisons among the GPW usability results from (1) the eye-tracking experiment, (2) questionnaire surveys and (3) the ready-made performance evaluation report validate the effectiveness of eye-tracking-based GPW usability evaluation.Originality/valueThe work contributes to shifting the GPW usability evaluation approach from a subjective judgment paradigm to an objective paradigm, as well as provides implications for enhancing GPW usability, including improving search function, reducing website complexity and prioritizing user needs.
Digital transformation, financing constraints and enterprise performance
Authors: Liu Meiyu , Li Haiyan , Li Chengyou , Yan Zhaojun 
Published: December 2023

PurposeThe main purpose of this paper is to explore the impact of digital transformation on enterprises' performance considering financing constraints in the capital market to explore whether digital transformation improves enterprises' performance through the financing constraints channel.Design/methodology/approachThis study, using a panel data set of 14,669 observations of 2,858 non-financial enterprises that issued A shares on the Shanghai and Shenzhen stock exchanges from 2013 to 2019, theoretically and empirically tests the impact and mechanism of digital transformation on enterprise performance.FindingsDigital transformation has a significant positive effect on enterprise performance; this conclusion remains the same after the robustness test and endogeneity problems are dealt with. Financing constraints play a mediation role between digital transformation and enterprise innovation. The effect of digital transformation on enterprise performance varies significantly by size, ownership and industry.Originality/valueThe theoretical contributions of this study not only enrich the literature on the economic benefits and mechanism of digital transformation but also expand the literature on the factors that influence enterprise performance. The practical contribution of this study is the reference that it provides for implementing decisions about enterprise digital transformation and formulating differentiated policies for government digital transformation.
A review of in-memory computing for machine learning: architectures, options
Authors: Snasel Vaclav , Dang Tran Khanh , Kueng Josef , Kong Lingping 
Published: December 2023

Purpose
This paper aims to review in-memory computing (IMC) for machine learning (ML) applications from history, architectures and options aspects. In this review, the authors investigate different architectural aspects and collect and provide our comparative evaluations.

Design/methodology/approach
Collecting over 40 IMC papers related to hardware design and optimization techniques of recent years, then classify them into three optimization option categories: optimization through graphic processing unit (GPU), optimization through reduced precision and optimization through hardware accelerator. Then, the authors brief those techniques in aspects such as what kind of data set it applied, how it is designed and what is the contribution of this design.

Findings
ML algorithms are potent tools accommodated on IMC architecture. Although general-purpose hardware (central processing units and GPUs) can supply explicit solutions, their energy efficiencies have limitations because of their excessive flexibility support. On the other hand, hardware accelerators (field programmable gate arrays and application-specific integrated circuits) win on the energy efficiency aspect, but individual accelerator often adapts exclusively to ax single ML approach (family). From a long hardware evolution perspective, hardware/software collaboration heterogeneity design from hybrid platforms is an option for the researcher.

Originality/value
IMC’s optimization enables high-speed processing, increases performance and analyzes massive volumes of data in real-time. This work reviews IMC and its evolution. Then, the authors categorize three optimization paths for the IMC architecture to improve performance metrics.
Investigating the role of flexibility as a moderator between supply chain integration and firm performance: the case of manufacturing sector
Authors: Jum'a Luay , Bushnaq Malak 
Published: December 2023

PurposeThe study aims to examine the impact of three types of supply chain integration (SCI) on supply chain flexibility (SCF), investigate the impact of SCF on supply chain performance (SCP) and analyse the indirect impact of SCI on SCP by considering the mediating role of SCF within the manufacturing sector of Jordan.Design/methodology/approachThis study used a quantitative approach to validate the study model. An online self-completed questionnaire was used to gather data from 219 participants from managers in various Jordanian manufacturing firms. SmartPLS software was used to perform structural equation modelling to test the formulated hypotheses.FindingsBased on the findings of the study, firms in Jordan's manufacturing sector would benefit from developing an integrative and flexible supply chain to boost SCP in the present volatile, uncertain, complex and speculative market. In addition, SCP was significantly influenced by investments in supply chain management practices related to SCI and SCF. Moreover, SCF significantly moderated the relationship between SCI and SCP. Thus, SCI and SCF assisted firms in reaching their highest potential performance through increased productivity, decreased expenses and increased satisfaction of their customers.Research limitations/implicationsThe study employed a cross-sectional design using SCF as a single construct. Future research should look into the specific type of SCFs that have an immense effect on SCP and how these types are affected by the three types of SCI. Furthermore, future research ought to employ probability sampling techniques to improve the generalizability of results or using a longitudinal data-collection design. Finally, additional research should be conducted to validate the findings of this study by replicating it in other specific industries or countries.Originality/valueThe study fills an identified gap based on previous studies by exploring the linkages between SCI, SCF and SCP in the context of manufacturing sector. Moreover, based on the relational view theory, the study proposed an assessment mechanism for SCP for firms based on the link between three types of SCI and SCF.
Exploring consumer webrooming behaviour in the emerging fashion market: an integrated approach and forthcoming research
Authors: Gopi Rajendra Kumar , Lavuri Rambabu , Sudhakar K Francis 
Published: December 2023

PurposeThe purpose of the study is to explore the role of affective commitment (AC) consumer empowerment on webrooming behaviour (WB) in a multichannel context of the fashion industry, with mediating (attitude [ATT]) and moderating (product involvement [PT]) effect. We used the stimulus– organism–responses theory as a theoretical underpinning.Design/methodology/approachWe collected 307 responses from fashionable consumer who was purposed fashion products recently through convenience sampling approach and analysed the data with structural equation modelling and PROCESS macro.FindingsThe results illustrated that AC and consumer empowerment had a significant impact on consumer attitude and their WB. Likewise, consumer attitude had a positive mediating association between AC, consumer empowerment and WB. PI significantly moderated the relationship between ACs, consumer empowerment with attitude and attitude with WB.Originality/valueThis study is one of the new research works of its kind, which examines the role of AC and consumer empowerment on WB in the fashion industry. This study contributes to the growing amount of literature on fashion marketing by analysing the rapidly growing phenomena of WB in a multichannel context of the fashion industry.
A critical analysis of large scale research infrastructures’ performances
Authors: Nocella Isabella , Linzalone Roberto , Ammirato Salvatore , Felicetti Alberto Michele 
Published: December 2023

Purpose
Large scale research infrastructures (LSRIs) are rising in the competitive and globalized research environment, since they offer to external researchers-users, inputs and services for cutting-edge, large scale researches. Such researches would not be possible with usual infrastructures and budgets of single universities and research institutions. However, despite the strategic relevance acknowledged to LSRI by the nascent literature and by national policymakers, there is a lack of understanding of configurations and key performances of a LSRI. This paper aims to bridge this gap by identifying key morphologies of LSRIs and analysing their performances.

Design/methodology/approach
The research is carried out adopting a mixed research methodology, merging a literature review with a survey conducted on a sample of 11 LSRIs; they provided the data set for the parametrization of a morphological matrix.

Findings
The research led to the identification of seven LSRIs morphologies, with different performance linked to their structure.

Originality/value
To the best of the authors’ knowledge, this paper originally proposes the data set needed to develop a morphological analysis of LSRIs.
FromPeople's KingtoKing of a Country: The Development of the Place Element Modifying the Title Paired in Apposition with a Personal Name Traced in thePeterborough Chronicle
Authors: Shinkawa Seiji 
Published: December 2023

AbstractA king was typically referred to aspeople's kingin the early period of Old English, whereas today,king of a countryis the most commonly used. This study attempts to trace the development of the place element modifying the title paired in apposition with a personal name in the tradition of vernacular English historiography as represented by thePeterborough Chronicle. There existed a strong tendency to specify a smaller land area (city, town, etc.) with the genitive form of the name of the place or with a prepositional phrase involving the place‐name and a larger one (kingdom, shire, etc.) with the genitive form of the name of the people living there in Early Old English. In later periods, however, the prepositional phrase was left as the only place element available for either a smaller or larger land area. Various prepositions heading the prepositional phrase became limited during the Old English period to just one in Early Middle English. Thus, the shift frompeople's kingtoking of a countrywas almost complete in Early Middle English. These findings are related to more general patterns of syntactic change in the noun phrase in English to highlight their current relevance.
Moving beyond the political trust crisis debate: Residual analyses to understand trends in political trust
Authors: VAN DER MEER T.W.G. , VAN ERKEL P.F.A. 
Published: December 2023

AbstractFor decades, political scientists have hotly debated longitudinal trends in political trust rates. An important undercurrent in the debate is that any decline in political trust might signal a legitimacy crisis. Yet, descriptive figures are unable to distinguish between two interpretations of these downward trends: (i) declines that can reasonably be expected as a reflection of declining political trustworthiness (i.e., procedural or output performance) and thereby reflect critical citizens who monitor their democratic institutions; and (ii) downward trends that are not warranted by democratic performance and thereby suggest a more fundamental disconnect between citizens and their democratic institutions.This research note argues that residuals to multilevel models of political trust allow us to distinguish between these two types, and thereby provide a better understanding of trends in political trust. These residuals do not only reveal short‐term aberrations to the explanatory model (often reflecting short‐lived, country‐specific events), but also the extent to which a country's trust rate systematically underperforms in the middle‐ to long‐term. To the extent that declining trust rates are lower than explanatory models predict, the residuals express excessive distrust. To the extent that declining trust rates are in line with the explanatory models, the residuals reflect critical, monitoring citizenship.We outline the approach of residual analyses as a tool to better understand trends in political trust. We illustrate the use of these residual analyses on a cross‐national, longitudinal data set (the Eurobarometer), covering 15 Western and Southern European countries between 1999 and 2019. While political trust rates fluctuate in all these countries, we only find evidence for a structural decline in two of these countries. In France and Spain political trust failed to recover in line with improving economic and institutional performance after the Great Recession. We then test the versatility of the tool to different conditions, including retests on an alternative set of countries (11 Central and Eastern European countries between 2004 and 2019) and an alternative dataset with different measures and time points (the European Social Survey).Finally, we elaborate on the two main conditions under which residual analyses offer a useful tool to the trend debate in political trust research: (1) a firm understanding of the object‐driven determinants of political trust, and (2) a detailed coverage of country‐wave combinations to separate structural trends from short‐term fluctuations.