bluuebunny/crossref_metadata_2025_split huggingface dataset:
340B Contract pharmacy growth by pharmacy ownership: 2009–2022
Authors: McGlave Claire , Bruno John P , Watts Elizabeth , Nikpay Sayeh 
Published: December 2023

Abstract
The 340B program grants eligible health care providers (“covered entities”) access to discounted prices for outpatient prescription drugs. Covered entities frequently rely on retail pharmacies (“contract pharmacies”) to dispense discounted drugs. This analysis describes contract pharmacy participation by ownership: the top 4 chains, grocery chains, small chains, and institutional independent pharmacies. We found that 71% of pharmacies in the top 4 chains were contract pharmacies. Forty one percentage of institutional pharmacies, 38% of grocery store pharmacies, and 22% of independent pharmacies participated in 340B in 2022. The median number of contracts per pharmacy was 2 among the top 4 chains and grocery store pharmacies vs 1 for all other pharmacy types. The median farthest distance in miles from contracting covered entities was largest for the top 4 chains (19 miles) and small chains (18 miles) and smallest for independent and institutional pharmacies (10 miles). The top 4 chains held the highest proportion of contracts with core safety-net providers (75% vs 61% of institutional pharmacies).
On best p-norm approximation of discrete data by polynomials
Authors: Floater Michael S 
Published: December 2023

Abstract
In this note, we derive a solution to the problem of finding a polynomial of degree at most $n$ that best approximates data at $n+2$ points in the $l_{p}$ norm. Analogous to a result of de la Vallée Poussin, one can express the solution as a convex combination of the Lagrange interpolants over subsets of $n+1$ points, and the error oscillates in sign.
Mitochondrial DNA mutations in HIV-exposed uninfected infants following the cessation of triple antiretroviral drugs
Authors: Lian Yuting , Wang Ailing , Wei Lai , Yao Jun , Bulloch Gabriella , Wu Zunyou , Zhao Yan 
Published: December 2023

Abstract

Objectives
Mitochondrial mutations in HIV-exposed uninfected (HEU) infants after cessation of ART are rarely studied. We analysed a group of HEU newborns born to mothers with late HIV diagnosis who received three doses of ART immediately after birth. We observed mitochondrial DNA (mtDNA) mutations at different times of withdrawal.

Methods
The study was based on a clinical trial conducted from 2015 to 2020. Newborns of the intervention group who met the criteria for this study received triple antiretroviral drugs, zidovudine + lamivudine + nevirapine, within 2 h after the birth, as post-partum prophylaxis, and at 14 days were switched to zidovudine + lamivudine + lopinavir/ritonavir, which was continued until 6 weeks of age. From August to November 2019, blood samples from HEU infants were also collected after ceasing 12 months of ART, and analysed for mtDNA.

Results
Our study found that mtDNA mutations remained prevalent in HEU infants a few years after three ARTs were stopped immediately after birth. Among them, D-loop, ND1 and CYTB are the first three mutated regions during different withdrawal periods. This pattern of mutations is similar to, but not exactly consistent with, HIV-infected children receiving standard ART.

Conclusions
Further studies are needed to determine the effects of these mutations on the development of HEU infants and whether stopping ART leads to the restoration of mitochondrial function.
Effects of static magnetic field on the sulfate metabolic pathway involved in Magnetospirillum magneticum AMB-1 cell growth and magnetosome formation
Authors: Chen Haitao , Shi Hongkai , Chen Changyou , Jiao Yangkun , Wang Pingping , Chen Chuanfang , Li Jinhua , Wu Long-Fei , Song Tao 
Published: December 2023

Abstract

Aims
Magnetotactic bacteria (MTB) can use their unique intracellular magnetosome organelles to swim along the Earth’s magnetic field. They play important roles in the biogeochemical cycles of iron and sulfur. Previous studies have shown that the applied magnetic fields could affect the magnetosome formation and antioxidant defense systems in MTB. However, the molecular mechanisms by which magnetic fields affect MTB cells remain unclear. We aim to better understand the dark at 28°C–29°C for 20 h, as shownthe interactions between magnetic fields and cells, and the mechanism of MTB adaptation to magnetic field at molecular levels.

Methods and results
We performed microbiological, transcriptomic, and genetic experiments to analyze the effects of a weak static magnetic field (SMF) exposure on the cell growth and magnetosome formation in the MTB strain Magnetospirillum magneticum AMB-1. The results showed that a 1.5 mT SMF significantly promoted the cell growth but reduced magnetosome formation in AMB-1, compared to the geomagnetic field. Transcriptomic analysis revealed decreased expression of genes primarily involved in the sulfate reduction pathway. Consistently, knockout mutant lacking adenylyl-sulfate kinase CysC did no more react to the SMF and the differences in growth and Cmag disappeared. Together with experimental findings of increased reactive oxidative species in the SMF-treated wild-type strain, we proposed that cysC, as a key gene, can participate in the cell growth and mineralization in AMB-1 by SMF regulation.

Conclusions
This study suggests that the magnetic field exposure can trigger a bacterial oxidative stress response involved in AMB-1 growth and magnetosome mineralization by regulating the sulfur metabolism pathway. CysC may serve as a pivotal enzyme in mediating sulfur metabolism to synchronize the impact of SMF on both growth and magnetization of AMB-1.
Academic machine learning researchers’ ethical perspectives on algorithm development for health care: a qualitative study
Authors: Kasun Max , Ryan Katie , Paik Jodi , Lane-McKinley Kyle , Dunn Laura Bodin , Roberts Laura Weiss , Kim Jane Paik 
Published: December 2023

Abstract

Objectives
We set out to describe academic machine learning (ML) researchers’ ethical considerations regarding the development of ML tools intended for use in clinical care.

Materials and Methods
We conducted in-depth, semistructured interviews with a sample of ML researchers in medicine (N = 10) as part of a larger study investigating stakeholders’ ethical considerations in the translation of ML tools in medicine. We used a qualitative descriptive design, applying conventional qualitative content analysis in order to allow participant perspectives to emerge directly from the data.

Results
Every participant viewed their algorithm development work as holding ethical significance. While participants shared positive attitudes toward continued ML innovation, they described concerns related to data sampling and labeling (eg, limitations to mitigating bias; ensuring the validity and integrity of data), and algorithm training and testing (eg, selecting quantitative targets; assessing reproducibility). Participants perceived a need to increase interdisciplinary training across stakeholders and to envision more coordinated and embedded approaches to addressing ethics issues.

Discussion and Conclusion
Participants described key areas where increased support for ethics may be needed; technical challenges affecting clinical acceptability; and standards related to scientific integrity, beneficence, and justice that may be higher in medicine compared to other industries engaged in ML innovation. Our results help shed light on the perspectives of ML researchers in medicine regarding the range of ethical issues they encounter or anticipate in their work, including areas where more attention may be needed to support the successful development and integration of medical ML tools.
Confidence score: a data-driven measure for inclusive systematic reviews considering unpublished preprints
Authors: Tong Jiayi , Luo Chongliang , Sun Yifei , Duan Rui , Saine M Elle , Lin Lifeng , Peng Yifan , Lu Yiwen , Batra Anchita , Pan Anni , Wang Olivia , Li Ruowang , Marks-Anglin Arielle , Yang Yuchen , Zuo Xu , Liu Yulun , Bian Jiang , Kimmel Stephen E , Hamilton Keith , Cuker Adam , Hubbard Rebecca A , Xu Hua , Chen Yong 
Published: December 2023

Abstract

Objectives
COVID-19, since its emergence in December 2019, has globally impacted research. Over 360 000 COVID-19-related manuscripts have been published on PubMed and preprint servers like medRxiv and bioRxiv, with preprints comprising about 15% of all manuscripts. Yet, the role and impact of preprints on COVID-19 research and evidence synthesis remain uncertain.

Materials and Methods
We propose a novel data-driven method for assigning weights to individual preprints in systematic reviews and meta-analyses. This weight termed the “confidence score” is obtained using the survival cure model, also known as the survival mixture model, which takes into account the time elapsed between posting and publication of a preprint, as well as metadata such as the number of first 2-week citations, sample size, and study type.

Results
Using 146 preprints on COVID-19 therapeutics posted from the beginning of the pandemic through April 30, 2021, we validated the confidence scores, showing an area under the curve of 0.95 (95% CI, 0.92-0.98). Through a use case on the effectiveness of hydroxychloroquine, we demonstrated how these scores can be incorporated practically into meta-analyses to properly weigh preprints.

Discussion
It is important to note that our method does not aim to replace existing measures of study quality but rather serves as a supplementary measure that overcomes some limitations of current approaches.

Conclusion
Our proposed confidence score has the potential to improve systematic reviews of evidence related to COVID-19 and other clinical conditions by providing a data-driven approach to including unpublished manuscripts.
Rumen fermentation of meal-fed sheep in response to diets formulated to vary in fiber and protein degradability
Authors: Sujani Sathya , Gleason Claire B , dos Reis Barbara R , White Robin R 
Published: December 2023

Abstract
The concentration of volatile fatty acid (VFA) provides an imprecise view of VFA dynamics due to the confounding effects of fluid pool size and dynamics. Determination of VFA flux using isotope is expensive and a complex methodology. Therefore, a rapid and affordable approach to explore VFA dynamics may allow comprehensive characterization of VFA availability. The objective of this study was to explore the use of VFA dynamics generated by meal feeding to derive time-series rates of VFA apparent appearance and disappearance driven by different protein and fiber sources. Six ruminally cannulated wethers were fed diets containing timothy hay or beet pulp (TH and BP) and soybean meal (SBM) or heated soybean meal (HSBM). Diets were, TH + HSBM; TH + SBM; BP + HSBM; and BP + SBM and the experimental design was a partially replicated 4 × 4 Latin Square. Concentrations of VFA and polyethylene glycol (PEG) in rumen fluid samples were estimated. Concentrations of PEG were used to estimate fluid passage and volume to calculate VFA mass, and fluid-mediated exit. Maximum apparent appearance rate (mmol/h), the rate of apparent appearance decline (mmol/mmol/h), mean apparent appearance flux (mmol/h), mean apparent disappearance (mmol/h), and apparent disappearance rate (mmol/mmol/h) were estimated by deriving a 1 pool model for each VFA on a mass basis where appearance was assumed to follow an exponential decay pattern and disappearance followed mass-action kinetics. Statistical analyses were conducted using a linear mixed effect regression with fixed effects for fiber source, protein source, and their interaction, as well as random effects for animal and period. Rumen fluid volume (L) was greater in HSBM diets (P = 0.033) and fluid passage (%/h) was greater in SBM diets (P = 0.048). Concentrations (higher acetate and butyrate, P = 0.002 and 0.004, respectively) and molar proportions (higher valerate, P = 0.035) of VFA were affected only by fiber source; however, protein source and fiber source interacted to significantly influence apparent appearance rates and absorption rates of many major VFA. On a flux basis, HSBM supported significantly elevated mean disappearance of propionate (P = 0.033). This data demonstrates that time-series evaluation of fermentation dynamics, including fluid dynamics and VFA concentrations can be used to estimate apparent appearance and disappearance of VFA. Although further work is needed to confirm the alignment of these estimates with measurements of VFA supplies to the animal, this modeling approach may provide a simpler way to better understand the kinetics of rumen.
2-Methoxybenzaldehyde effectively repels ants
Authors: Kay Tomas , Siegenthaler Georges , Kench Timothy , Keller Laurent 
Published: December 2023

AbstractAnts can particularly make for harmful pests, infesting human homes and reducing crop yields. The damage caused by ants and the efforts to mitigate the damage are hugely costly. Broad-spectrum insecticides are used most commonly; however, due to their negative side effects, there is increasing interest in nontoxic alternatives. One promising commercially available alternative is 2-hydroxybenzaldehyde, which is naturally produced by various arthropods as a means of chemical defense and effectively repels ants. Here we conduct a structure–activity relationship investigation, testing how different chemical modifications alter the repellence of 2-hydroxybenzaldehyde. We find that 2-methoxybenzaldehyde is considerably more effective than 2-hydroxybenzaldehyde at repelling the common black garden ant, Lasius niger. We next compare the most effective repellent chemicals against 4 particularly harmful ant species to confirm that the results obtained with L. niger are general to ants and that our results are relevant to mitigate the costs of ant damage.
Physician reported outcomes of hip arthroscopy without a perineal post: an international survey
Authors: Volpi Alexander , Hopkins Kristin Twomey , McHugh Malachy , Galano Gregory 
Published: December 2023

ABSTRACT
Although the current literature reports an acceptable rate of complications with the use of a perineal post in hip arthroscopy, they are still possible and preventable. The purpose of this study was to survey International Society for Hip Arthroscopy (ISHA) members on their use of postless distraction in hip arthroscopy. A 19-question survey was emailed to hip preservation surgeons that are members of ISHA. The questions examined surgeons’ location, experience, utilization of a perineal post or postless distraction and any complications they may have encountered. In all, 145 respondents completed the survey. Regarding complications encountered when using a perineal post, the most frequent responses were temporary nerve damage (115, 80.6%), temporary genitourinary complications (39, 27%), temporary genital skin injury (35, 24%) and permanent nerve injury (12, 8%). Regarding the postless technique, of the 60 respondents who noted they have utilized postless distraction, 9 (15%) reported complications, with 7 (12%) reporting temporary nerve damage being the most common and 0 reporting cases of permanent nerve injury. These were statistically significantly less than those reported with a perineal post. Ninety-seven percent reported that after utilizing postless distraction, their patients were recovering better than or the same as when using a perineal post. This survey had excellent international participation by experienced hip arthroscopists. There were a statistically significantly decreased number of complications reported by the surgeons utilizing postless distraction. This survey highlights that postless distraction is being done successfully with lower reported complications and excellent patient recovery.
Why Care About Freedom and Agency?
Authors: Prueitt Catherine 
Published: December 2023

AbstractIn ethical systems that focus on apportioning praise and blame, a key consideration is often whether or not the individual is a free agent since individuals are only held to be responsible for what they freely choose. As various critiques indicate, if it were to be the case that freedom is in some way illusory or radically restricted, these systems would have a significant problem since reactive attitudes would involve holding individuals responsible for actions that they did not freely choose. I will argue that the problem may run even deeper: even if there is such a thing as free agency, it is a mistake to think that autonomous individuals uniquely instantiate this agency. I will draw on arguments from Pratyabhijñā Śaivism, which state that although there is ultimately no such thing as a praise or blameworthy individual agent, free agency is the precondition for manifestation itself. Worlds, not individuals, are the proper unit of analysis for ethical theories. This position picks up on many of the critiques of the kind of substantial self that stands apart from the world that were offered by various Buddhist traditions in the Classical Sanskritic context. At the same time, it does not fall prey to these objections precisely because the self that Pratyabhijñā theorists argue for is neither an unchanging substance nor a minimally thin kind of self-awareness that could be accommodated by no-self theorists. Pratyabhijñā theorists’ particular way of understanding agency, then, presents a productive exchange between some of the most ethically salient ramifications of Buddhist no-self theories and insights into why, nevertheless, freedom and agency are inextricably bound up in our worlds.
The distribution of triatomine (Hemiptera: Reduviidae) vectors of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Missouri: historical records and specimen submissions from community science programs
Authors: Santos Ellen M , Santanello Catherine D , Curtis-Robles Rachel , Killets Keswick , Lawrence Gena , Sevenshadows Jet , Mahoney Meredith J , Baker Molly , Hamer Sarah A 
Published: December 2023

Abstract
Triatomine species (kissing bugs) infected with Trypanosoma cruzi are found across the southern United States. The northern limits of Trypanosoma cruzi infected kissing bugs are less understood. The objective of this work was to describe the locations of kissing bugs from Illinois and Missouri based on historical records, submissions to Texas A&M University’s (TAMU) Kissing Bug Community Science Program and the Centers for Disease Control and Prevention (CDC), and records from online platforms (iNaturalist, BugGuide, and GBIF) up to and including 2022. A total of 228 records were discovered, including 186 from historical or observation platforms and 42 specimens submitted to TAMU or CDC. Species included Triatoma sanguisuga (221 total records, 9 nymphs) and Triatoma lecticularia (7 records). Notably, nearly all (24/26) records submitted to TAMU were collected indoors. Twelve of the 30 (40%) specimens tested were positive for the presence of T. cruzi, including parasite discrete taxonomic units TcI and TcIV. One triatomine sample had been found in a bed feeding on the submitter; this bug was positive for T. cruzi and had evidence of human blood in its gut. Records suggest a ubiquitous distribution in Missouri and potentially to the northernmost border in Illinois. Further investigations into triatomine distribution and infection status are needed within states assumed to be northern limits in order to create public health and veterinary health messaging and baseline distributional maps from which to measure future range shifts in relation to a changing climate.
Trade-offs in growth and reproduction of rhizomatous clonal plant Phragmites communis in response to aeolian processes
Authors: Ba Chaoqun , Zhai Shanshan , Qian Jianqiang , Liu Bo , Zhu Jinlei , Liu Zhimin 
Published: December 2023

Abstract
Trade-offs in growth and reproduction are essential parts of the adaptive strategies of clonal plants. How rhizomatous psammophytes respond to aeolian processes (sand burial and wind erosion) by means of trade-offs is supposed to be especially important for their colonization on the active sand dune. Despite partial documentation of the responses of rhizomatous species to aeolian processes, how these clonal species respond to aeolian processes by means of potential trade-offs in growth and reproduction still remains unclear. In this study, we employed field investigation and biomass modeling to evaluate the trade-offs between vegetative and reproductive growth as well as between the number and size of ramets of Phragmites communis in response to sand burial and wind erosion. Sand burial enhanced the accumulation of seed biomass and reproductive effort. Wind erosion reduced reproductive effort but had no significant influence on seed biomass. Sand burial increased the biomass of ramets, while wind erosion increased ramet population density and accelerated ramet maturation. Our results demonstrate that rhizomatous psammophytes adjust their growth strategies in response to aeolian processes, i.e. reproductive growth and ramet size increase in response to sand burial, while vegetative growth and ramet numbers increase in response to wind erosion.
CRISPR/Cas9 editing of Downy mildew resistant 6 (DMR6-1) in grapevine leads to reduced susceptibility to Plasmopara viticola
Authors: Djennane Samia , Gersch Sophie , Le-Bohec Françoise , Piron Marie-Christine , Baltenweck Raymonde , Lemaire Olivier , Merdinoglu Didier , Hugueney Philippe , Nogué Fabien , Mestre Pere 
Published: December 2023

Abstract
Downy mildew of grapevine (Vitis vinifera), caused by the oomycete Plasmopara viticola, is an important disease that is present in cultivation areas worldwide, and using resistant varieties provides an environmentally friendly alternative to fungicides. DOWNY MILDEW RESISTANT 6 (DMR6) from Arabidopsis is a negative regulator of plant immunity and its loss of function confers resistance to downy mildew. In grapevine, DMR6 is present in two copies, named VvDMR6-1 and VvDMR6-2. Here, we describe the editing of VvDMR6-1 in embryogenic calli using CRISPR/Cas9 and the regeneration of the edited plants. All edited plants were found to be biallelic and chimeric, and whilst they all showed reduced growth compared with non-transformed control plants, they also had reduced susceptibility to P. viticola. Comparison between mock-inoculated genotypes showed that all edited lines presented higher levels of salicylic acid than controls, and lines subjected to transformation presented higher levels of cis-resveratrol than controls. Our results identify VvDMR6-1 as a promising target for breeding grapevine cultivars with improved resistance to downy mildew.
Mutator-like transposable element 9A interacts with metacaspase 1 and modulates the incidence of Al-induced programmed cell death in peanut
Authors: Luo Shuzhen , Li Ailing , Luo Jin , Liao Guoting , Li Xia , Yao Shaochang , Wang Aiqin , Xiao Dong , He Longfei , Zhan Jie 
Published: December 2023

Abstract
The toxicity of aluminum (Al) in acidic soil inhibits plant root development and reduces crop yields. In the plant response to Al toxicity, the initiation of programmed cell death (PCD) appears to be an important mechanism for the elimination of Al-damaged cells to ensure plant survival. In a previous study, the type I metacaspase AhMC1 was found to regulate the Al stress response and to be essential for Al-induced PCD. However, the mechanism by which AhMC1 is altered in the peanut response to Al stress remained unclear. Here, we show that a nuclear protein, mutator-like transposable element 9A (AhMULE9A), directly interacts with AhMC1 in vitro and in vivo. This interaction occurs in the nucleus in peanut and is weakened during Al stress. Furthermore, a conserved C2HC zinc finger domain of AhMULE9A (residues 735–751) was shown to be required for its interaction with AhMC1. Overexpression of AhMULE9A in Arabidopsis and peanut strongly inhibited root growth with a loss of root cell viability under Al treatment. Conversely, knock down of AhMULE9A in peanut significantly reduced Al uptake and Al inhibition of root growth, and alleviated the occurrence of typical hallmarks of Al-induced PCD. These findings provide novel insight into the regulation of Al-induced PCD.