bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multimodal assessment of mitochondrial function in Parkinson's disease
Authors: Payne Thomas , Burgess Toby , Bradley Stephen , Roscoe Sarah , Sassani Matilde , Dunning Mark J , Hernandez Dena , Scholz Sonja , McNeill Alisdair , Taylor Rosie , Su Li , Wilkinson Iain , Jenkins Thomas , Mortiboys Heather , Bandmann Oliver 
Published: December 2023

AbstractThe heterogenous aetiology of Parkinson's disease is increasingly recognized; both mitochondrial and lysosomal dysfunction have been implicated. Powerful, clinically applicable tools are required to enable mechanistic stratification for future precision medicine approaches. The aim of this study was to characterize bioenergetic dysfunction in Parkinson's disease by applying a multimodal approach, combining standardized clinical assessment with midbrain and putaminal 31-phosphorus magnetic resonance spectroscopy (31P-MRS) and deep phenotyping of mitochondrial and lysosomal function in peripheral tissue in patients with recent-onset Parkinson's disease and control subjects.Sixty participants (35 patients with Parkinson's disease and 25 healthy controls) underwent 31P-MRS for quantification of energy-rich metabolites [ATP, inorganic phosphate (Pi) and phosphocreatine] in putamen and midbrain. In parallel, skin biopsies were obtained from all research participants to establish fibroblast cell lines for subsequent quantification of total intracellular ATP and mitochondrial membrane potential (MMP) as well as mitochondrial and lysosomal morphology, using high content live cell imaging.Lower MMP correlated with higher intracellular ATP (r = −0.55, P = 0.0016), higher mitochondrial counts (r = −0.72, P &lt; 0.0001) and higher lysosomal counts (r = −0.62, P = 0.0002) in Parkinson's disease patient-derived fibroblasts only, consistent with impaired mitophagy and mitochondrial uncoupling. 31P-MRS-derived posterior putaminal Pi/ATP ratio variance was considerably greater in Parkinson's disease than in healthy controls (F-tests, P = 0.0036). Furthermore, elevated 31P-MRS-derived putaminal, but not midbrain Pi/ATP ratios (indicative of impaired oxidative phosphorylation) correlated with both greater mitochondrial (r = 0.37, P = 0.0319) and lysosomal counts (r = 0.48, P = 0.0044) as well as lower MMP in both short (r = −0.52, P = 0.0016) and long (r = −0.47, P = 0.0052) mitochondria in Parkinson's disease. Higher 31P-MRS midbrain phosphocreatine correlated with greater risk of rapid disease progression (r = 0.47, P = 0.0384).Our data suggest that impaired oxidative phosphorylation in the striatal dopaminergic nerve terminals exceeds mitochondrial dysfunction in the midbrain of patients with early Parkinson's disease. Our data further support the hypothesis of a prominent link between impaired mitophagy and impaired striatal energy homeostasis as a key event in early Parkinson's disease.
The ‘Common Law Method’: British Approaches to the Development of International Law
Authors: Hovell Devika 
Published: December 2023

Abstract
For better or for worse, the ‘English school’ or ‘British tradition’ of international law has eluded systematization or definition. This article examines the responses of a UK legal adviser, a British judge on the International Court of Justice and influential scholars to a particular case, the Corfu Channel case. In doing so, it is possible to identify clear synergies in the mainstream legal method of British international lawyers. It should not be surprising that this method follows in the common law tradition, displaying its three key hallmarks of connection to social practice, focus on courts and an anti-theoretical tendency. Identity and analysis of these characteristics helps us to understand the distinctive contribution of British approaches to international law and the work this ‘common law method’ has done in strengthening and shaping international law. Identifying these characteristics is also important in order to understand the more problematic implications of their application in the international legal context. The common law method has consequences for the structure and direction of the international legal system, including the parameters of its community, the site of its authority and the role of theory in its development. Reflection on these strengths and weaknesses helps us better understand British contributions to international law. Paradoxically, the route to a more universal international law requires us first to understand the ways in which it is plural.
Methotrexate did not add skin cancer risk in patients with psoriasis receiving narrowband ultraviolet B phototherapy: a nationwide retrospective cohort study
Authors: Chao Chun-Hsien , Wu Chun-Ying , Chou Fan-Ling , Chen Yi-Ju 
Published: December 2023

Abstract

Background
In the era of biologic therapy, phototherapy and methotrexate (MTX) are still commonly used for treatment in patients with moderate-to-severe psoriasis. However, the skin cancer risk following a combination of MTX and narrowband ultraviolet B (NB-UVB) has rarely been explored.

Objectives
To investigate whether MTX plus NB-UVB increases skin cancer risk in patients with psoriasis.

Methods
We conducted a retrospective cohort study of data in the Taiwan National Health Insurance Research Database from 1997 to 2013. Cumulative incidences and multivariate analysis were investigated using a competing risk regression model, comparing skin cancer risk between cohorts of people having combination therapy and those using NB-UVB alone, matched for relative confounders. We further conducted a sensitivity analysis for those receiving a higher MTX dose. Standardized incidence ratios (SIRs) were calculated for skin cancer risk.

Results
We enrolled 3203 participants in each cohort. No significant differences in skin cancers were noted between the two cohorts for the cumulative incidences (log-rank test, P = 0.28) and for the hazard ratio (HRs) [adjusted HR 0.50, 95% confidence interval (CI) 0.15–1.63, P = 0.247] in the competing risk regression model. There were also no significant differences between those receiving higher-dose MTX and UVB alone in the cumulative incidences of skin cancers (P = 0.23) and the HR (adjusted HR = 0.29, 95% CI 0.04–2.21, P = 0.23) in the multivariate analysis. There was no significant difference in the SIR between the two cohorts compared with the general population.

Conclusions
In the Taiwanese population, MTX does not increase skin cancer risk in patients with moderate-to-severe psoriasis receiving NB-UVB.
Age-Specific Resurgence in Invasive Pneumococcal Disease Incidence in the COVID-19 Pandemic Era and Its Association With Respiratory Virus and Pneumococcal Carriage Dynamics: A Time-Series Analysis
Authors: Rybak Alexis , Assad Zein , Levy Corinne , Bonarcorsi Stéphane , Béchet Stéphane , Werner Andreas , Wollner Alain , Valtuille Zaba , Kaguelidou Florentia , Angoulvant François , Cohen Robert , Varon Emmanuelle , Ouldali Naïm 
Published: December 2023

Abstract
Using multiple national surveillance systems, we found an increase in the incidence of invasive pneumococcal disease during after the relaxation of non-pharmaceutical interventions against COVID-19, which strongly varied by age. Age groups with higher incidence of respiratory syncytial virus and influenza also experienced higher increase in invasive pneumococcal disease incidence, with no change in pneumococcal carriage.
Oral health professional intervention and child physical abuse—European legal approach
Authors: Corte-Real Ana , Almiro Pedro Armelim , Silva Mafalda , Nunes Tiago , Abreu João , Carreira Carla , Vieira Duarte Nuno 
Published: December 2023

Abstract

Physical violence against children and adolescents is an issue of Global Public Health. This study aims to identify traumatic injuries and the medicolegal temporary framework of the victim’s profile in the European legal approach. Participants and setting include the following: the clinical reports of a Portuguese European Clinical Academic Center database were analysed. An observational and prospective cohort study was performed. A descriptive analysis of the variables was conducted, considering gender, bimodal age groups, place of residence, offender data, place of occurrence, aetiology, localization, type of injuries, personal injury assessment by Quantum doloris, and injury time. The statistical analysis was performed by Spearman’s rho and Kendall’s tau-b correlation tests, Pearson’s chi-square test of independence (χ2), and Mann–Whitney and Kruskal–Wallis nonparametric tests (P &lt; 0.05). The relationship between age groups and the place of occurrence was statistically significant (P = 0.001). Orofacial and nonorofacial injuries were related (P = 0.035). The General Data Protection Regulation is not a barrier to the treatment and sharing of justified data but a framework for safeguarding individuals’ fundamental rights, including the Right to Health. Meticulous reporting of the clinical situation involves the victim, the occurrence, and the potential offender.

Key points
Estimating geodynamic model parameters from geodetic observations using a particle method
Authors: Marsman C P , Vossepoel F C , van Dinther Y , Govers R 
Published: December 2023

SUMMARY
Bayesian-based data assimilation methods integrate observational data into geophysical forward models to obtain the temporal evolution of an improved state vector, including its uncertainties. We explore the potential of a variant, a particle method, to estimate mechanical parameters of the overriding plate during the interseismic period. Here we assimilate vertical surface displacements into an elementary flexural model to estimate the elastic thickness of the overriding plate, and the locations and magnitudes of line loads acting on the overriding plate to produce flexure. Assimilation of synthetic observations sampled from a different forward model than is used in the particle method, reveal that synthetic seafloor data within 150 km from the trench are required to properly constrain parameters for long wavelength solutions of the upper plate (i.e. wavelength ∼500 km). Assimilation of synthetic observations sampled from the same flexural model used in the particle method shows remarkable convergence towards the true parameters with synthetic on-land data only for short to intermediate wavelength solutions (i.e. wavelengths between ∼100 and 300 km). In real-data assimilation experiments we assign representation errors due to discrepancies between our incorrect or incomplete physical model and the data. When assimilating continental data prior to the 2011 Mw Tohoku-Oki earthquake (1997–2000), an unrealistically low effective elastic plate thickness for Tohoku of ∼5–7 km is estimated. Our synthetic experiments suggest that improvements to the physical forward model, such as the inclusion of a slab, a megathrust interface and viscoelasticity of the mantle, including accurate seafloor data, and additional geodetic observations, may refine our estimates of the effective elastic plate thickness. Overall, we demonstrate the potential of using the particle method to constrain geodynamic parameters by providing constraints on parameters and corresponding uncertainty values. Using the particle method, we provide insights into the data network sensitivity and identify parameter trade-offs.
New constraints on the shear wave velocity structure of the Ivrea geophysical body from seismic ambient noise tomography (Ivrea-Verbano Zone, Alps)
Authors: Scarponi M , Kvapil J , Plomerová J , Solarino S , Hetényi G 
Published: December 2023

SUMMARY
We performed seismic ambient noise tomography to investigate the shallow crustal structure around the Ivrea geophysical body (IGB) in the Ivrea-Verbano Zone (IVZ). We achieved higher resolution with respect to previous tomographic works covering the Western Alps, by processing seismic data collected by both permanent and temporary seismic networks (61 broad-band seismic stations in total). This included IvreaArray, a temporary, passive seismic experiment designed to investigate the IVZ crustal structure. Starting from continuous seismic ambient noise recordings, we measured and inverted the dispersion of the group velocity of surface Rayleigh waves (fundamental mode) in the period range 4–25 s. We obtained a new, 3-D vS model of the IVZ crust via the stochastic neighbourhood algorithm (NA), with the highest resolution between 3 to 40 km depth. The fast and shallow shear wave velocity anomaly associated with the IGB presents velocities of 3.6 km s−1 directly at the surface, in remarkable agreement with the location of the exposed lower-to-middle crustal and mantle outcrops. This suggests a continuity between the surface geological observations and the subsurface geophysical anomalies. The fast IGB structure reaches vS of 4 km s−1 at 20–25 km depth, at the boundary between the European and Adriatic tectonic plates, and in correspondence with the earlier identified Moho jump in the same area. The interpretation of a very shallow reaching IGB is further supported by the comparison of our new results with recent geophysical investigations, based on receiver functions and gravity anomaly data. By combining the new geophysical constraints and the geological observations at the surface, we provide a new structural interpretation of the IGB, which features lower crustal and mantle rocks at upper crustal depths. The comparison of the obtained vS values with the physical properties from laboratory analysis of local rock samples suggests that the bulk of the IGB consists of a combination of mantle peridotite, ultramafic and lower crustal rocks, bound in a heterogeneous structure. These new findings, based on vS tomography, corroborate the recent interpretation for which the Balmuccia peridotite outcrops are continuously linked to the IGB structure beneath. The new outcomes contribute to a multidisciplinary framework for the interpretation of the forthcoming results of the scientific drilling project DIVE.
DIVE aims at probing the lower continental crust and its transition to the mantle, with two ongoing and one future boreholes (down to 4 km depth) in the IVZ area, providing new, complementary information on rock structure and composition across scales. In this framework, we constrain the upper crustal IGB geometries and lithology based on new evidence for vS, connecting prior crustal knowledge to recent active seismic investigations.
Simultaneous Bayesian estimation of size-specific catchability and size spectrum parameters from trawl data
Authors: Krumsick Kyle J , Pedersen Eric J 
Published: December 2023

Abstract
Fisheries-independent surveys are a critical tool for monitoring marine populations and communities. However, considerations must be made to account for variable-size-based catchability. The size-specific catchability function is therefore key for estimating size distributions, but often requires extensive data sets or specialized field experiments to determine. We develop a Bayesian model capable of simultaneously estimating both a size-based catchability curve and species-specific size spectrum parameters from trawl data by assuming that individual species size spectra follow a theoretically derived parametric size spectrum model. The resulting model provides a means of estimating catchability and size spectra within an adaptive framework capable of accommodating confounding factors such as vessel power and fish density, potentially allowing for improved biomass and productivity estimates. We demonstrate the application of this model using 15 years of Greenland Halibut (Reinhardtius hippoglossoides) survey data from Nunavut to determine size-specific catchabilities and assess whether the size spectrum of Greenland Halibut has changed across the time series. While size spectrum parameters for this stock were not found to vary, we did find evidence of time-varying catchability parameters across the study period.
Circle Actions on Four Dimensional Almost Complex Manifolds With Discrete Fixed Point Sets
Authors: Jang Donghoon 
Published: December 2023

Abstract
We establish a necessary and sufficient condition for pairs of integers to arise as the weights at the fixed points of an effective circle action on a compact almost complex 4-manifold with a discrete fixed point set. As an application, we provide a necessary and sufficient condition for a pair of integers to arise as the Chern numbers of such an action, answering negatively a question by Sabatini whether $c_{1}^{2}[M] \leq 3 c_{2}[M]$ holds for any such manifold $M$. We achieve this by demonstrating that pairs of integers that arise as weights of a circle action also arise as weights of a restriction of a $\mathbb {T}^{2}$-action. Furthermore, we discuss applications to circle actions on complex/symplectic 4-manifolds and semi-free circle actions with discrete fixed point sets.
A Bedaquiline, Pyrazinamide, Levofloxacin, Linezolid, and Clofazimine Second-line Regimen for Tuberculosis Displays Similar Early Bactericidal Activity as the Standard Rifampin-Based First-line Regimen
Authors: Zainabadi Kayvan , Vilbrun Stalz Charles , Mathurin Laurent Daniel , Walsh Kathleen Frances , Pape Jean William , Fitzgerald Daniel W , Lee Myung Hee 
Published: December 2023

Abstract

Background
In 2018 the World Health Organization recommended a switch to an all oral bedaquiline-based second-line regimen for treatment of drug-resistant tuberculosis (DR-TB). How these new second-line regimens fare in comparison to first-line regimens for treatment of drug-sensitive tuberculosis (DS-TB) is not well known.

Methods
In this study, we contemporaneously enrolled subjects with DS-TB (n = 31) or DR-TB (n = 23) and assessed their response to therapy with first-line (rifampin, isoniazid, ethambutol, pyrazinamide) or second-line (bedaquiline, pyrazinamide, levofloxacin, linezolid, clofazimine) regimens, respectively.

Results
We found that the early bactericidal activity of first- and second-line regimens was similar during the first 2 weeks of therapy as determined by BACTEC MGIT, colony-forming units, and a liquid limiting dilution assay capable of detecting differentially detectable/culturable Mycobacterium tuberculosis. Furthermore, an identical percentage (77.8%) of subjects from the DS-TB and DR-TB cohorts converted to culture negative after 2 months of therapy.

Conclusions
Despite presenting with more advanced disease at time of treatment, subjects with DR-TB receiving an all oral bedaquiline-based second-line treatment regimen displayed a similar microbiological response to therapy as subjects with DS-TB receiving a first-line treatment regimen.
Investigation of the transport and metabolic patterns of oil-displacing bacterium FY-07-G in the microcosm model using X-CT technology
Authors: Zhao Xueqing , Liao Zitong , Liu Tongtong , Cheng Wei , Gao Ge , Yang Mingbo , Ma Ting , Li Guoqiang 
Published: December 2023

Abstract

Aims
Microbial enhanced oil recovery (MEOR) is dedicated to enhancing oil recovery by harnessing microbial metabolic activities and their byproducts within reservoir rocks and fluids. Therefore, the investigation of microbial mobility and their extensive distribution within crude oil is of paramount importance in MEOR. While microscale models have been valuable for studying bacterial strain behavior in reservoirs, they are typically limited to 2D representations of porous media, making them inadequate for simulating actual reservoir conditions. Consequently, there is a critical need for 3D models and dependable visualization methods to observe bacterial transport and metabolism within these complex reservoir environments.

Methods and results
Bacterial cellulose (bc) is a water-insoluble polysaccharide produced by bacteria that exhibits biocompatibility and biodegradability. It holds significant potential for applications in the field of MEOR as an effective means for selective plugging and spill prevention during oil displacement processes. Conditionally cellulose-producing strain, FY-07-G, with green fluorescent labeling, was engineered for enhanced oil recovery. 3D micro-visualization model was constructed to directly observe the metabolic activities of the target bacterial strain within porous media and to assess the plugging interactions between cellulose and the medium. Additionally, X-ray computed tomography (X-CT) technology was employed for a comprehensive analysis of the transport patterns of the target strain in oil reservoirs with varying permeabilities. The results indicated that FY-07-G, as a microorganism employing biopolymer-based plugging principles to enhance oil recovery, selectively targets and seals regions characterized by lower permeability and smaller pore spaces.

Conclusions
This work provided valuable insights into the transport and metabolic behavior of MEOR strains and tackled the limitation of 2D models in faithfully replicating oil reservoir conditions, offering essential theoretical guidance and insights for the further application of oil-displacing bacterial strains in MEOR processes.
An update on the prevalence of colistin and carbapenem-resistant Gram-negative bacteria in aquaculture: an emerging threat to public health
Authors: Memesh Roa , Yasir Muhammad , Ledder Ruth G , Zowawi Hosam , McBain Andrew J , Azhar Esam I 
Published: December 2023

Abstract
Aquaculture has been recognized as a hotspot for the emergence and spread of antimicrobial resistance genes conferring resistance to clinically important antibiotics. This review gives insights into studies investigating the prevalence of colistin and carbapenem resistance (CCR) among Gram-negative bacilli in aquaculture. Overall, a high incidence of CCR has been reported in aquatic farms in several countries, with CCR being more prevalent among opportunistic human pathogens such as Acinetobacter nosocomialis, Shewanella algae, Photobacterium damselae, Vibrio spp., Aeromonas spp., as well as members of Enterobacteriaceae family. A high proportion of isolates in these studies exhibited wide-spectrum profiles of antimicrobial resistance, highlighting their multidrug-resistance properties (MDR). Several mobile colistin resistance genes (including, mcr-1, mcr-1.1, mcr-2, mcr-2.1, mcr-3, mcr-3.1, mcr-4.1, mcr-4.3, mcr-5.1, mcr-6.1, mcr-7.1, mcr-8.1, and mcr-10.1) and carbapenemase encoding genes (including, blaOXA-48, blaOXA-55, blaNDM, blaKPC, blaIMI, blaAIM, blaVIM, and blaIMP) have been detected in aquatic farms in different countries. The majority of these were carried on MDR Incompatibility (Inc) plasmids including IncA/C, and IncX4, which have been associated with a wide host range of different sources. Thus, there is a risk for the possible spread of resistance genes between fish, their environments, and humans. These findings highlight the need to monitor and regulate the usage of antimicrobials in aquaculture. A multisectoral and transdisciplinary (One Health) approach is urgently needed to reduce the spread of resistant bacteria and/or resistance genes originating in aquaculture and avoid their global reach.