bluuebunny/crossref_metadata_2025_split huggingface dataset:
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase
Authors: Xu Yifeng , Crowe Kerstin B. , Lieske Paulena L. , Barnes Michael , Bandara Kalpanie , Chu Jianlin , Wei Wei , Scarcelli John J. , Zhang Lin 
Published: February 2024

AbstractSite‐specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual‐site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to generate and compare two RMCE hosts, one (dFRT) compatible with the Flp recombinase, the other (dBxb1) compatible with the Bxb1 recombinase. Our comparison demonstrated that the dBxb1 host was able to generate stable transfectant pools in a shorter time frame, and cells within the dBxb1 transfectant pools were more phenotypically and genotypically stable. We further improved process performance of the dBxb1 host, resulting in desired fed batch performance attributes. Clones derived from this improved host (referred as 41L‐11) maintained stable expression profiles over extended generations. While the data represents a significant improvement in the efficiency of our cell line development process, the dual LP architecture also affords a high degree of flexibility for development of complex protein modalities.
Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess
Authors: Ma Wei , Li Fengyi , Li Longyue , Li Bin , Niu Kangle , Liu Qinghua , Han Laichuang , Han Lijuan , Fang Xu 
Published: February 2024

AbstractWe designed and constructed a green and sustainable bioprocess to efficiently coproduce D‐tagatose, bioethanol, and microbial protein from whey powder. First, a one‐pot biosynthesis process involving lactose hydrolysis and D‐galactose redox reactions for D‐tagatose production was established in vitro via a three‐enzyme cascade. Second, a nicotinamide adenine dinucleotide phosphate‐dependent galactitol dehydrogenase mutant, D36A/I37R, based on the nicotinamide adenine dinucleotide‐dependent polyol dehydrogenase from Paracoccus denitrificans was created through rational design and screening. Moreover, an NADPH recycling module was created in the oxidoreductive pathway, and the tagatose yield increased by 3.35‐fold compared with that achieved through the pathway without the cofactor cycle. The reaction process was accelerated using an enzyme assembly with a glycine–serine linker, and the tagatose production rate was 9.28‐fold higher than the initial yield. Finally, Saccharomyces cerevisiae was introduced into the reaction solution, and 266.5 g of D‐tagatose, 162.6 g of bioethanol, and 215.4 g of dry yeast (including 38% protein) were obtained from 1 kg of whey powder (including 810 g lactose). This study provides a promising sustainable process for functional food (D‐tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.
Relationship between face‐to‐face and non‐face‐to‐face communication, and well‐being in older volunteers during the pandemic: The REPRINTS project
Authors: Fujihira Kyoko , Takahashi Tomoya , Sagara Tomoya , Matsunaga Hiroko , Fujita Koji , Suzuki Hiroyuki , Murayama Hiroshi , Fujiwara Yoshinori 
Published: February 2024

AbstractVolunteering older adults plays a role in maintaining well‐being through facilitation of face‐to‐face communication. However, the COVID‐19 pandemic reduced volunteer opportunities involving physical interaction among seniors. While studies have reported that indirect communication such as non‐face‐to‐face can support and improve mental health, it remains unclear whether it can substitute face‐to‐face communication among older volunteers accustomed to frequent interpersonal interactions. Therefore, the current study aimed to examine the relationship between communication forms and well‐being during the pandemic by communication partner, among volunteers aged 65 years and above. Participants engaged in REPRINTS, which are volunteer who read picture books to children. At questionnaire survey was completed to investigate changes in the frequency of face‐to‐face and non‐face‐to‐face communication before and during the pandemic, and well‐being using the WHO‐5. The results showed that maintaining or increasing the frequency of face‐to‐face communication with friends and neighbours before to during the pandemic was associated with a lower risk of being determined poor well‐being (ORs: 0.465, 95% CI: 0.274–0.789, p = .004). This study demonstrated the significance of creating opportunities for face‐to‐face communication to maintain the well‐being of older volunteers during the pandemic.
Marfan syndrome in a Ghanaian male: The diagnostic challenges
Authors: Folson Aba A. , Agyapong Kwabena Oteng , Dey Dzifa , Eghan Philip , Quaye Billey 
Published: February 2024

Key Clinical MessageMarfan syndrome (MFS) is an autosomal dominantly inherited condition that has varying phenotypic expressions. This case report describes one such African patient, from Ghana, who had typical clinical and imaging traits of MFS but was first diagnosed incidentally at the age of 23 years. In this report, we explore the challenges of early diagnosis in this population.
Optimization of Ca/Si ratio in schists to enhance the pozzolanic supplementary cementitious material for OPC blending
Authors: Mokhtari Pozhhan , Hassannezhad Kosar , Bundur Zeynep Basaran , Donmez Sezen , Gulgun Mehmet Ali , Kriven Waltraud M. 
Published: February 2024

AbstractSupplementary cementitious materials (SCMs) are partial substitutes for ordinary Portland cement (OPC) to reduce cost, the production of carbon dioxide (CO2), and fossil fuel consumption. Recent studies illustrated the effectiveness of calcined kaolinite clay as a proper partial replacement for OPC. Hence, schist‐type materials containing sufficient clay contents are regarded as promising candidates for OPC replacement. This study focuses on the possible use of activated calcium carbonates (CaCO3) to augment pozzolanic reactions of schists as a substitute for OPC. The use of activated calcium carbonates proved to be successful in expediting pozzolanic reactions and facilitating the formation of additional calcium aluminosilicate hydrate (C‐A‐S‐H). In this study, the optimal strength performance for blended cement was achieved by adjusting the total carbonate content to 30 wt.% of the schist. The decision to introduce carbonate to the schist was guided by calculations determining the need for an excess of calcium ions to fully harness the potential for C‐A‐S‐H formation. Virgin and augmented schists were activated by up to 80% of their respective potentials by heat treatment. Phase content and decomposition behavior of the active components were investigated by thermogravimetric analysis (TGA), x‐ray diffraction (XRD), and scanning electron microscopy (SEM). Cement paste samples incorporating 30 wt.% replacement of OPC with activated schist SCMs were prepared. The phase distribution and compressive strengths of these samples were assessed at hydration intervals of 2, 7, 28, 50, and 90 days. Cement formulations with activated calcium‐augmented SCMs exhibited compressive strength values at 109%, 101%, 96%, 95%, and 94% compared to pure cement paste at 2, 7, 28, 50, and 90 days, respectively. These values surpassed 90% of the compressive strength of pure OPC at equivalent hydration time points.
Fish and coral assemblages of a highly isolated oceanic island: The first eDNA survey of the Ogasawara Islands
Authors: Açıkbaş Ayşe Haruka Oshima , Narisoko Haruhi , Huerlimann Roger , Nishitsuji Koki , Satoh Noriyuki , Reimer James Davis , Ravasi Timothy 
Published: February 2024

AbstractThe Ogasawara Islands are a highly isolated oceanic archipelago in the Pacific Ocean that possess unique faunal and floral biodiversity with a high level of endemism. As historically more focus has been put on the terrestrial realm in examining diversification and evolutionary processes on oceanic islands, publicly accessible and spatially resolved data of marine reef ecosystems remain scarce. To address this issue, we conducted the first environmental DNA (eDNA) metabarcoding surveys of the actinopterygian (ray‐finned) and elasmobranch fishes and of Scleractinia coral assemblages in the waters of the Ogasawara Islands. We detected a total of 124 unique taxa of fish and 38 unique taxa of scleractinian corals. Overall, our eDNA results confirmed that the Ogasawara Islands host a rich variety of coral and fish fauna and underline the strength of eDNA surveys in rapidly obtaining targeted multi‐taxa data using seawater samples, requiring comparatively little effort and a lack of requirement for in situ taxonomic expertise. We anticipate that continued biomonitoring using eDNA with high sampling effort will add to and complement the body of knowledge regarding species distributions, invasive species, and biodiversity hotspots within oceanic archipelagos.
Enhancing betacyanin stability: Comparison of dragon fruit (Hylocereus polyrhizus) pulp and peel powders through encapsulation technology during storage
Authors: Nurhadi Bambang , Qonit Muhammad Abdillah Hasan , Mubarok Syariful , Saputra Rudy Adi 
Published: February 2024

AbstractBetacyanin can be found in the peel or pulp of dragon fruit. As a natural pigment, betacyanin is unstable, so it requires encapsulation technology to maintain its quality. The stability of encapsulated betacyanin from dragon fruit peel compared to dragon fruit pulp has yet to be discovered. This study aims to compare the stability of encapsulated betacyanin (with maltodextrin and gum Arabic) from dragon fruit peel and pulp dried with vacuum drying. Dragon fruit peel extraction utilized a 50% aqueous ethanol solvent, while pulp juice extraction was performed. The ratio of dragon fruit extract to coating materials was set at 1:3 (solid/solid). Research shows that dragon fruit juice powder had higher stability and phytochemical concentrations than the ethanol extract of dragon fruit peel powder during 30 days of storage. Despite similar color stability (similar range value of ΔE), the color from dragon fruit juice powder more closely resembled the natural fruit, albeit with weaker antioxidant activity than the peel powder. Betacyanin concentration in juice powder was notably higher (82.56–156.82 μg/g) than in the ethanol extract of dragon fruit peel powder (52.51–75.12 μg/g). A combination of maltodextrin and Arabic gum (1:1) as coating materials demonstrated the highest concentrations of total phenolic and total betacyanin (81.15–95.87 mg/g and 121.91–156.82 μg/g, respectively) during the storage period. These findings contribute to our comprehension of betacyanin stability and functionality, facilitating precise applications in industrial processing environments based on their source attributes.
Pistachio green hull and pomegranate peel extracts as two natural antiglycation agents
Authors: Roudbari Mozhgan , Barzegar Mohsen , Sahari Mohammad Ali 
Published: February 2024

AbstractAdvanced glycation end products (AGEs) are formed in the final step of the nonenzymatic Maillard reaction, which can contribute to various health problems such as diabetes mellitus, renal failure, and chronic inflammation. Bioactive compounds with antiglycation properties have the potential to inhibit AGE‐related diseases. This study investigated the antiglycation potential of pistachio green hull (PGH) and pomegranate peel (PP) extracts, which are polyphenol‐rich agro‐residues, against fluorescent AGE formation and compared the results with pyridoxine (vitamin B6), metformin, and EDTA (as usual chemical antiglycation agents). The results showed that PGH and PP effectively inhibited the formation of AGEs in bovine serum albumin–glucose (BSA‐Glu) and BSA–fructose (BSA‐Fru) with antiglycation activities ranging from 92% to 97%. PP extract (with an IC50 of 94 mg ml−1) had a greater antiglycation ability than PGH extract (with an IC50 of 142 mg ml−1). Also, results indicated that the antiglycation activities of the extracts were comparable to that of pyridoxine, and higher than metformin and EDTA. These findings suggest that the two studied extracts can be used for sustainable production of high‐added‐value food products with a positive effect on consumers' health.
Improving fresh‐cut fruit salad quality and longevity with chitosan coating enriched with poppy seed phenolics
Authors: Demircan Bahar , Velioglu Yakup Sedat 
Published: February 2024

AbstractThis innovative study introduces the application of a 5% (v/v) poppy seed phenolic extract‐infused edible chitosan coating on fresh‐cut fruit salads (comprising apple, pineapple, pomegranate, and kiwi) stored at +4°C for 12 days. Non‐coated samples experienced notable changes: 4.30% weight loss, 25% decay, pH level at 3.59, titratable acidity of 0.18%, and browning index of 1.71. In contrast, fruit salads coated with chitosan–poppy seed phenolic extract exhibited significant improvements: weight loss reduced to 3.10%, decay limited to 3.13%, pH increased to 3.76, titratable acidity enhanced to 0.20%, and browning index notably decreased to 0.33. Soluble solids ranged from 11.83 to 14.71, L* from −8.13 to 18.64, a* from −1.85 to 22.35, and b* from 8.26 to 27.89 in non‐coated salads. Adding poppy seed phenolic extract to the coated fruits slightly expanded these ranges. Sensory evaluations consistently rated non‐coated samples between 1 and 3, while the coated samples received higher ratings between 6 and 7. These assessments consistently highlighted enhanced attributes, including intensified aroma, enriched color, improved taste, texture, and overall acceptability. Moreover, incorporating poppy seed phenolic extract amplified sensory qualities and significantly improved microbial safety (<106 CFU/g). In summary, the chitosan‐based coating, enriched with poppy seed phenolic extract, effectively extended the shelf life of fresh‐cut fruit salads. This integrated approach preserves key attributes, ensures microbial quality, and enhances the sensory characteristics of these products. The study's results emphasize its potential as a pivotal innovation in food preservation by providing specific and tangible outcomes.
Anti‐Toxoplasma gondii activity of rose hip oil–solid lipid nanoparticles
Authors: Mohammad Rahimi Hanieh , Hesari Zahra , Mirsamadi Elnaz Sadat , Nemati Sara , Mirjalali Hamed 
Published: February 2024

AbstractToxoplasma gondii is a highly prevalent pathogen, reported from almost all geographical regions of the world. Current anti‐T. gondii drugs are not effective enough in immunocompromised patients, encephalitis, chorioretinitis, and congenital toxoplasmosis. Therefore, the prescription of these drugs has been limited. Rose hip oil (RhO) is a natural plant compound, which shows antibacterial, anticancer, and anti‐inflammatory activities. In the current study, the anti‐T. gondii and cell toxicity effects of solid lipid nanoparticles (SLNs) loaded by RhO (RhO‐SLNs) were evaluated. Emulsification sonicated‐homogenization method was used to prepare SLNs. RhO‐SLNs were characterized, and their anti‐T. gondii and cell toxicity effects were evaluated using in vitro analyses. The particle size and the zeta potential of the nanoparticles were 152.09 nm and −15.3 mV nm, respectively. The entrapment efficiency percentage was 79.1%. In the present study, the inhibitory concentration (IC)50 against T. gondii was >1 μg/mL (p‐value <.0001). The cell toxicity assay showed cytotoxicity concentration (CC)50 >10 mg/mL (p‐value = .017). In addition, at least 75% of T. gondii‐infected Vero cells remained alive at concentrations >10 mg/mL. The concentration of 1 mg/mL showed highest anti‐Toxoplasma activity and lowest cell toxicity against the Vero cell. Our findings suggest that carrying natural plant compounds with SLNs could be considered an effective option for treatment strategies against T. gondii infections.
A bibliometric analysis of the research status and trends in studies on polymyositis and dermatomyositis with interstitial lung disease from 2000 to 2022 using Web of Science
Authors: Ma Xiao‐Na , Feng Wei , Chen Shu‐Lin , Zhong Xiao‐Qin , Zheng Xue‐Xia , Lin Chang‐Song , Xu Qiang 
Published: February 2024

AbstractBackgroundThe main subtypes of idiopathic inflammatory myopathies (IIMs)—polymyositis (PM) and dermatomyositis (DM)—are often presented as interstitial lung disease (ILD) in clinical practice; therefore, many researchers have combined the three studies into PM/DM with ILD.MethodsUsing bibliometrics, the research status, progress, and hotspots of PM/DM with ILD between 2000 and 2022 were studied. Literature data on PM/DM with ILD were retrieved from the Web of Science (WoS) database for the research period. Visualization software, including VOSviewer, Pajek, CiteSpace, and Scimago Graphica were used for bibliometric analysis.ResultsA total of 1555 relevant articles were obtained, and the overall research in this field showed an increasing trend. Regarding contributing countries and venues, Japan published the most articles while Rheumatology was the most prolific journal. Regarding authors, the most published article was by Wang Guochun from Changchun University of Technology in China. Keyword analysis and cocited literature cluster analysis showed that diagnosis, classification, autoantibodies, antibodies, prognosis, complications, and treatment of PM/DM with ILD have been hot topics in this field recently. Moreover, our study shows that anti‐mda5 antibody, mortality, gene 5 antibody, IIMs, double‐blind, and prognostic factors, among others, may be new hot topics.ConclusionThis study found that research on PM/DM with ILD has increased over time, and scholars are paying more attention to this field. The development of new drugs for the management, treatment, and prevention of PM/DM with ILD is the primary task of researchers and a direction for future research in this field.
Cotyledon‐Specific Flow Evaluation of Rhesus Macaque Placental Injury Using Ferumoxytol Dynamic Contrast‐Enhanced MRI
Authors: Chen Ruiming , Seiter Daniel , Keding Logan T. , Vazquez Jessica , Antony Kathleen M. , Simmons Heather A. , Basu Puja , Mejia Andres F. , Johnson Kevin M. , Stanic Aleksandar K. , Liu Ruo‐Yu , Shah Dinesh M. , Golos Thaddeus G. , Wieben Oliver 
Published: February 2024

BackgroundRecently, dynamic contrast‐enhanced (DCE) MRI with ferumoxytol as contrast agent has recently been introduced for the noninvasive assessment of placental structure and function throughout. However, it has not been demonstrated under pathological conditions.PurposeTo measure cotyledon‐specific rhesus macaque maternal placental blood flow using ferumoxytol DCE MRI in a novel animal model for local placental injury.Study TypeProspective animal model.SubjectsPlacental injections of Tisseel (three with 0.5 mL and two with 1.5 mL), monocyte chemoattractant protein 1 (three with 100 μg), and three with saline as controls were performed in a total of 11 rhesus macaque pregnancies at approximate gestational day (GD 101). DCE MRI scans were performed prior (GD 100) and after (GD 115 and GD 145) the injection (term = GD 165).Field Strength/Sequence3 T, T1‐weighted spoiled gradient echo sequence (product sequence, DISCO).AssessmentSource images were inspected for motion artefacts from the mother or fetus. Placenta segmentation and DCE processing were performed for the dynamic image series to measure cotyledon specific volume, flow, and normalized flow. Overall placental histopathology was conducted for controls, Tisseel, and MCP‐1 animals and regions of tissue infarctions and necrosis were documented. Visual inspections for potential necrotic tissue were conducted for the two Tisseelx3 animals.Statistical TestsWilcoxon rank sum test, significance level P < 0.05.ResultsNo motion artefacts were observed. For the group treated with 1.5 mL of Tisseel, significantly lower cotyledon volume, flow, and normalized flow per cotyledon were observed for the third gestational time point of imaging (day ~145), with mean normalized flow of 0.53 minute−1. Preliminary histopathological analysis shows areas of tissue necrosis from a selected cotyledon in one Tisseel‐treated (single dose) animal and both Tisseelx3 (triple dose) animals.Data ConclusionThis study demonstrates the feasibility of cotyledon‐specific functional analysis at multiple gestational time points and injury detection in a placental rhesus macaque model through ferumoxytol‐enhanced DCE MRI.Level of EvidenceNATechnical EfficacyStage 2
Clonal propagation, scale dependent assembly, and nucleation drives natural regeneration in a restinga sandy coastal plain
Authors: Matias Lidiane , Demetrio Guilherme Ramos , da Silva Milena Dutra , Moura Flávia 
Published: February 2024

AbstractOn the sandy coastal plains, environmental filters that limit seedling establishment and survival create a naturally heterogeneous landscape, formed by vegetation patches inserted in a matrix of bare sandy soil. The succession processes in these ecosystems depend on some pioneer species that facilitate the recruitment and survival of other species. Here, we evaluated the spatial pattern and changes of vegetation patches of restinga (i.e., coastal sandy plain vegetation) over one decade (2011–2021). The research was carried out in Alagoas State, Northeast of Brazil. We considered as patches the vegetation islands inserted in a matrix of bare sandy soil and applied methodologies of photointerpretation. Landscape metrics included patch area, edge, shape, and core area. To test the effect of landscape metrics on species occurrence and species richness per patch, we applied generalized linear models (GLM) with binomial and quasi‐Poisson distributions, respectively. We used the “betapart” package to evaluate the importance of turnover and nestedness as drivers of beta diversity among patches. Our data indicate that a key plant (Myrciaria floribunda), which can propagate by root suckers, initiates a succession process, creating regeneration nuclei, which seem to follow a deterministic succession pattern until the patch reaches 10 m2 in area. In the successional sequence, the patches grow and coalesce with neighboring patches. Species richness of patches presented an unimodal response to patch size. Overall, there is a greater contribution of species turnover to among‐patch beta diversity. However, for patches smaller than 10 m2, beta diversity was mostly driven by nestedness, reflecting a process of species addition to the growing patches. The succession pattern found in the study area should be considered for the formulation of nature‐based restoration models, expected to be more efficient and effective for ecosystems with similar environmental filters.