bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro and in vivo efficacy of a cosmetic product formulated with new lipid particles for the treatment of aged skin
Authors: Catalano Antonio , Mitri Khalil , Perugini Paola , Condrò Giorgia , Sands Caroline 
Published: October 2023

AbstractBackgroundThe cumulative oxidative damage causes an acceleration in the skin aging.ObjectivesTo evaluate the ability of a new patented matrix of lipid particles (SIREN CAPSULE TECHNOLOGY™) to have superior anti‐aging properties due to its high sensitivity to reactive oxygen species (ROS), testing its efficacy versus free or encapsulated vitamins.MethodsAn in vitro study was conducted to evaluate the protective effects of lipid particles using menadione as an enhancer of oxidative stress. Subsequently, in vivo studies evaluated skin hydration, skin barrier function, and smoothness and wrinkle depth. For this purpose, gels containing free or encapsulated vitamins were used as controls.ResultsIn vitro, the SIREN CAPSULE TECHNOLOGY™ gel shows inhibitory activity against ROS production through menadione induction. In fact, at both tested concentrations, ROS production is lower than in the control samples (placebo, free vitamins, encapsulated vitamins).In vivo, the net effect of SIREN CAPSULE TECHNOLOGY™ gel versus the others permitted to conclude that lipid particles exert a higher skin moisturizing effect (20.17%) and a stronger effect in reducing transepidermal water loss (−16.29%) after 4 weeks of treatment. As for surface analysis, a gel based on SIREN CAPSULE TECHNOLOGY™ improves the skin texture in a similar way than gel containing encapsulated vitamins (Ra and Rz variations in 4 weeks).ConclusionsSIREN CAPSULE TECHNOLOGY™ represents an advance and a successful strategy to develop cosmetic products for the treatment of skin conditions associated with an accumulation of ROS. SIREN CAPSULE TECHNOLOGY™ represents a result‐oriented breakthrough in the effective delivery of active ingredients to the skin.
Effect of different surface treatments on the retention force of additively manufactured interim implant‐supported crowns
Authors: Filokyprou Thaleia , Kesterke Matthew J. , Liu Xiaohua , Cho Seok‐Hwan , Revilla‐León Marta 
Published: October 2023

AbstractPurposeTo compare the effect of different pre‐cementation surface treatments and bonding protocols on the retention force of additively manufactured (AM) implant‐supported interim crowns.Material and methodsA total of 50 AM interim crowns (Temporary CB resin) were cemented on implant abutments. Five groups (n = 10) were established based on the different surface pre‐treatments performed in the intaglio surface of the specimens: no surface pre‐treatment (Group C or control), air‐abraded with 50‐μm aluminium oxide particles (Group AP), air‐abraded with 50‐μm aluminium oxide particles followed by the application of silane (Group AMP), silane (Group MP), and air‐abraded with 30 μm silica‐coated aluminum oxide particles followed by the application of silane (Group CMP). Each specimen was cemented into an implant abutment using a composite resin cement (Rely X Unicem2). Afterward, the specimens underwent retention testing with a Universal Instron machine. Pull‐off forces (N) and modes of failure were registered. Statistical analysis was performed using Mann‐Whitney U tests with Bonferroni corrections for multiple tests (α = 0.05).ResultsThe median retention force values were 233.27 ±79.28 N for Group Control, 398.59 ±68.59 N for Group MP, 303.21 ±116.80 N for Group AMP, 349.31 ±167.73 N for Group CMP, and 219.85 ± 55.88 N for Group AP. The pull‐off forces were significantly greater for Group MP, while the differences between the remaining groups were not statistically significant (P > 0.05). Group AP showed the lowest retention force values among all the groups. Failure modes after the pull‐off testing were predominantly adhesive and substrate failure of the AM interim material.ConclusionsThe surface treatment of the intaglio AM crown tested significantly influenced the retention force values measured. Pre‐treatment with an MDP‐containing silane improved the retentive force values computed, whereas pre‐treatment with 50‐μm Al2O3 air‐particle abrasion alone is not recommended prior to cementation on a titanium‐based implant abutment.
Characterization of Alternaria alternata isolates from different citrus species grown in Tunisian Cap Bon peninsula
Authors: Taycir Grati Affes , Walid Yeddes , Safa Rguez , Wissem Aidi Wannes , Majdi HammAmi , Salma Lasram , Synda Chenenaoui , Saber Khammessi , Bachkouel Sarra , Bouzid Nasraoui , Moufida Saidani Tounsi 
Published: October 2023

AbstractThe prospection of citrus trees affected by Alternaria alternata in Tunisian Cap Bon peninsula for 2 years, 2018 and 2019, showed a variation in the percentage of isolation frequency depending on the region, age of citrus trees and citrus species and varieties. Thirty isolates of A. alternata from citrus species were characterized and studied for their variability. The isolates were subjected to morphological identification using macroscopic and microscopic features and molecular characterization through PCR amplification of their internal transcribed spacer regions. A high morphological and molecular diversity within A. alternata isolates was detected. The molecular sequencing results precisely confirmed that these fungal isolates belong to A. alternata strains. Pathogenicity test showed that A. alternata T1 and T9 isolates were capable of causing disease symptoms only on young leaves of clementine plant (MA3 variety). These findings are useful in the development of sustainable strategies to manage Alternaria in citrus‐growing areas in Tunisia.
Melatonin suppresses tumor proliferation and metastasis by targeting GATA2 in endometrial cancer
Authors: Liao Yangyou , Li Ruiling , Pei Jingyuan , Zhang Juan , Chen Bo , Dong Haojie , Feng Xiaoyu , Zhang Hongshuo , Shang Yuhong , Sui Linlin , Kong Ying 
Published: October 2023

AbstractEndometrial cancer (EC) is a reproductive system disease that occurs in perimenopausal and postmenopausal women. However, its etiology is unclear. Melatonin (MT) has been identified as a therapeutic agent for EC; however, its exact mechanism remains unclear. In the present study, we determined that GATA‐binding protein 2 (GATA2) is expressed at low levels in EC and regulated by MT. MT upregulates the expression of GATA2 through MT receptor 1A (MTNR1A), whereas GATA2 can promote the expression of MTNR1A by binding to its promoter region. In addition, in vivo and in vitro experiments showed that MT inhibited the proliferation and metastasis of EC cells by upregulating GATA2 expression. The protein kinase B (AKT) pathway was also affected. In conclusion, these findings suggest that MT and GATA2 play significant roles in EC development.
Stakeholder perspectives on co‐designing a post‐registration mental health nursing curriculum: A case study
Authors: Molloy Renee , Hansen Alison , Robinson Eddie , D'Astoli Pauline , Wood Tom , Buus Niels 
Published: October 2023

AbstractIntroductionGlobally, healthcare regulatory bodies require providers of academic programs to involve service users in the design and delivery of education, yet concrete and tested directions for operationalising this are lacking. Service users' involvement in education is often passive, ad‐hoc and tokenistic, with limited input at the program development level.AimTo explore the co‐design processes and outcomes of a collaborative revision of an Australian post‐registration mental health nursing curriculum.MethodUsing qualitative case study design, the experiences and collaborative practices of five stakeholders (one mental health service user, two carers and two academics) co‐designing a post‐registration mental health nursing curriculum were explored.ResultsCommonly identified barriers to meaningful involvement were overcome through planning and collaborative execution of the group's work. Participants reported a strong sense of successful collaboration in reviewing the curriculum, trusting relationships and mutual respect.DiscussionWhile it was possible to support and augment stakeholder knowledge in the context of post‐registration education, co‐design processes were ultimately bound by the university's relatively inflexible bureaucratic context.Implications for PracticeTo ensure effective stakeholder collaboration beyond classroom teaching, mental health nurse academics must prepare service users for the role, explaining the language and practices of the tertiary health education and nursing context. As part of co‐design processes, organisational mandates must be explored and negotiated, and mutual clarity regarding expectations and roles must be established. A Design Thinking approach can support meaningful engagement of stakeholders in curriculum development.
Black cardamom (Amomum subulatum) extract improves growth potential, antioxidant status, immune parameters and response to crowding stress in Catla catla
Authors: Ali Wazir , Fatima Mahroze , Shah Syed Zakir Hussain , Khan Noor , Naveed Saima 
Published: October 2023

AbstractNatural herbs are excellent alternatives to synthetic compounds to enhance the growth performance and health status of fish. This study was conducted to evaluate the efficacy of black cardamom (Amomum subulatum) extract (BCE) as an herbal supplement in improving the growth, antioxidant status, haematology, and serum biochemistry of Catla catla. The acclimatized fish (N = 900; average initial weight = 14.44 ± 0.33 g) were allocated into five groups (60 fish/group in triplicate) in hapas (4 × 2 × 2.5 ft) and provided with feed containing 0 (control), 0.5, 1, 2 and 4 g/kg BCE for 90 days before being subjected to 8 days of crowding stress. After 90 days, significantly higher feed utilization and growth were observed in all BCE‐fed treatments compared to the control; however, the maximum values of these parameters were noted in the 2 g/kg BCE‐fed treatment. Moreover, the BCE‐fed groups exhibited a significant increase in antioxidant indices (glutathione peroxidase, superoxide dismutase and catalase), with a significant reduction in malondialdehyde levels, indicating a higher antioxidant capacity compared to the control. Significant improvements in haematological parameters, such as an increase in haematocrit, haemoglobin and red blood cells and a decrease in white blood cells, were observed in BCE‐fed treatments compared to the control. Furthermore, BCE‐fed groups showed a significant decrease in serum glucose, cortisol and triglycerides, while total protein, globulin and albumin levels were significantly higher compared with the control. During the feeding trial of 90 days, no mortality was observed; however, the lowest cumulative mortality was noted in the 2 g/kg BCE group after crowding stress of 8 days. In conclusion, the use of BCE in feed can enhance the growth performance, antioxidant status, haematology, and serum biochemistry of C. catla and improve the resistance against crowding stress. The optimum levels of BCE for C. catla were estimated based on weight gain % (1.78 g/kg; R2 = 0.97), feed conversion ratio (1.65 g/kg; R2 = 0.98), MDA content (1.66 g/kg; R2 = 0.93) and serum lysozyme activity (1.72 g/kg; R2 = 1) using broken‐line regression analysis.
The impact of a 40‐min nap on neuromuscular fatigue profile and recovery following the 5‐m shuttle run test
Authors: Boukhris Omar , Zghal Firas , Trabelsi Khaled , Suppiah Haresh , Ammar Achraf , Jahrami Haitham , Hsouna Hsen , Abdessalem Raouf , Glenn Jordan M. , Chtourou Hamdi , Driller Matthew 
Published: October 2023

SummaryThis study aims to investigate the impact of a 40‐min nap opportunity on perceived recovery, exertion, and maximal voluntary isometric contraction (MVIC) following the 5‐m shuttle run test (5SRT), after 1 night of normal sleep. In a randomised, counterbalanced, cross‐over design, 17 trained men (mean [SD] age 20 [3] years, height 173 [6] cm, body mass 68 [6] kg) performed a 5SRT under two conditions: a 40‐min nap opportunity and no‐nap condition. After both conditions, electromyography signals during a 5‐s isometric knee extension were recorded before and immediately after the 5SRT. Two electrical nerve stimulations at the femoral nerve were measured during and after the MVIC. Force, voluntary activation level, M‐wave amplitudes, potentiated twitch, and electromyography signals (root mean square) were measured during each MVIC. Perceived exertion was recorded after each repetition of the test and perceived recovery was determined after the end of the MVIC. Compared to the no‐nap condition, the 40‐min nap resulted in significant enhancements in both the highest distance (p < 0.01, Δ = +7.6%) and total distance (p < 0.01, Δ = +7.5%). Before and after exercise, values for MVIC, root mean square, M‐wave amplitudes, and voluntary activation level were improved after the 40‐min nap opportunity compared to no‐nap condition (all p ≤ 0.01). Values for perceived exertion and recovery were improved after the 40‐min nap opportunity in comparison with no‐nap condition (p ≤ 0.01). A 40‐min nap opportunity improved repeated high‐intensity short‐term maximal performance, perceived recovery, associated neuromuscular responses, and reduced perceived fatigue. Therefore, our findings suggest that central and peripheral processes are involved in the improvements of 5SRT performance after napping.
Sleep and the sleep electroencephalogram in C57BL/6 and C3H/HeN mice
Authors: van Dorp Rick , Rolleri Elisa , Deboer Tom 
Published: October 2023

SummaryDifferent mouse strains used in biomedical research show different phenotypes associated with their genotypes. Two mouse strains commonly used in biomedical sleep research are C57Bl/6 and C3H/He, the strains differ in numerous aspects, including their ability to secrete melatonin as well as the expression of several sleep‐related genes. However, sleep regulation has only limitedly been compared between C3H/HeN and C57Bl/6 mice. We therefore compared sleep–wake behaviour and EEG‐measured spectral brain activity for C57bl/6 and C3H/HeN mice during a 12:12 h light: dark baseline and during and after a 6 h sleep deprivation. The C3H mice spent more time in NREM sleep around the light–dark transition and more time in REM sleep during the dark phase compared with C57bl/6 mice. The C3H mice also showed more EEG activity in the 4.5–7.5 Hz range during all stages and a stronger 24 h modulation of EEG power density in almost all EEG frequencies during NREM sleep. After the sleep deprivation, C3H mice showed a stronger recovery response, which was expressed in both a larger increase in EEG slow wave activity (SWA) and more time spent in NREM sleep. We show large differences regarding sleep architecture and EEG activity between C3H and C57bl/6 mice. These differences include the amount of waking during the late dark phase, the 24 h amplitude in EEG power density, and the amount of REM sleep during the dark phase. We conclude that differences between mouse strains should be considered when selecting a model strain to improve the generalisability of studies investigating biomedical parameters related to sleep and circadian rhythms.
Associations of actigraphy‐assessed sleep variables with adiposity and serum cardiometabolic outcomes in emerging adults
Authors: McNeil Jessica , Berry Nathaniel T. , Dollar Jessica M. , Shriver Lenka H. , Keane Susan P. , Shanahan Lilly , Wideman Laurie 
Published: October 2023

SummaryThis study assessed associations of actigraphy‐assessed sleep with adiposity and serum cardiometabolic outcomes in emerging adults, and whether sex and race modified these associations. Data on 147 emerging adults (age = 19.4 ± 1.3 years; body mass index = 26.4 ± 7.0 kg m−2; 59% female; 65% White) from RIGHT Track Health were used. Actigraphy‐based sleep measures included sleep duration, sleep efficiency, sleep timing midpoint, day‐to‐day sleep duration and sleep timing midpoint variability. Combined sleep duration and sleep timing behaviours were also derived (early‐bed/late‐rise, early‐bed/early‐rise, late‐bed/late‐rise, late‐bed/early‐rise). Outcomes included body mass index and BodPod‐assessed fat mass index, fasting serum leptin, C‐reactive protein, and homeostatic model assessment‐insulin resistance. Sleep duration was 5.4 h per night. We noted an inverse association between sleep duration and homeostatic model assessment‐insulin resistance. The early‐bed/early‐rise group had greater body mass index, C‐reactive protein and homeostatic model assessment‐insulin resistance compared with the early‐bed/late‐rise group (referent). Sex modified associations of sleep efficiency with C‐reactive protein; stratified results revealed positive association between sleep efficiency and C‐reactive protein in males, but not females. Race modified associations of sleep duration with body mass index and leptin, and of sleep duration variability with C‐reactive protein. Stratified analyses revealed inverse associations between sleep duration with body mass index and leptin in Black, multiracial/other race individuals only. Positive association between sleep duration variability and C‐reactive protein was noted in White individuals only. Shorter sleep duration, particularly when combined with earlier sleep timing, is associated with greater adiposity and serum cardiometabolic outcomes. Additional studies are needed to assess individual‐ and contextual‐level factors that may contribute to sex and race differences in sleep health and cardiometabolic risk in emerging adults.
Response and survival of dogs with proteinuria (UPC > 2.0) treated with angiotensin converting enzyme inhibitors
Authors: Fulton Emily A. , McBrearty Alix R. , Shaw Darren J. , Ridyard Alison E. 
Published: October 2023

AbstractBackgroundAngiotensin‐converting enzyme inhibitors (ACEi) are a recommended treatment for glomerular proteinuria. Frequency of response to ACEi and the association of achieving proposed urine protein‐to‐creatinine ratio (UPC) targets on survival is unknown.ObjectivesTo determine response rates to ACEi therapy and whether a positive response is associated with improved survival.AnimalsEighty‐five dogs with proteinuria (UPC > 2.0).MethodsRetrospective study including dogs (UPC > 2.0) prescribed an ACEi for treatment of proteinuria. Baseline creatinine, albumin, cholesterol, UPC, and systolic blood pressure were recorded, and cases reviewed to track UPC. Treatment response was defined as achieving a UPC of <0.5 or reduction of ≥50% from baseline within 3 months. Outcome data were collected to determine overall and 12‐month survival.ResultsThirty‐five (41%) dogs responded to ACEi treatment. Treatment response was statistically associated with both median survival time (664 days [95% confidence interval (CI): 459‐869] for responders compared to 177 [95% CI: 131‐223] for non‐responders) and 12‐month survival (79% responders alive compared to 28% non‐responders). Baseline azotemia or hypoalbuminemia were also associated with a worse prognosis, with odds ratios of death at 12 months of 5.34 (CI: 1.85‐17.32) and 4.51 (CI: 1.66‐13.14), respectively. In the 25 dogs with normal baseline creatinine and albumin, response to treatment was associated with 12‐month survival (92% responders alive compared to 54% non‐responders, P = .04).Conclusions and Clinical ImportanceWhen the UPC is >2.0, achieving recommended UPC targets within 3 months appears to be associated with a significant survival benefit. Response to treatment is still associated with survival benefit in dogs with less severe disease (no azotemia or hypoalbuminemia).
Determining reinfection rates by hepatitis C testing interval among key populations: A systematic review and meta‐analysis
Authors: Munari Stephanie C. , Traeger Michael W. , Menon Vinay , Latham Ned H. , Manoharan Lakshmi , Luhmann Niklas , Baggaley Rachel , MacDonald Virginia , Verster Annette , Siegfried Nandi , Conway Brian , Klein Marina , Bruneau Julie , Stoové Mark A. , Hellard Margaret E. , Doyle Joseph S. 
Published: October 2023

AbstractBackground & AimsDetecting hepatitis C virus (HCV) reinfection among key populations helps prevent ongoing transmission. This systematic review aims to determine the association between different testing intervals during post‐SVR follow‐up on the detection of HCV reinfection among highest risk populations.MethodsWe searched electronic databases between January 2014 and February 2023 for studies that tested individuals at risk for HCV reinfection at discrete testing intervals and reported HCV reinfection incidence among key populations. Pooled estimates of reinfection incidence were calculated by population and testing frequency using random‐effects meta‐analysis.ResultsForty‐one single‐armed observational studies (9453 individuals) were included. Thirty‐eight studies (8931 individuals) reported HCV reinfection incidence rate and were included in meta‐analyses. The overall pooled estimate of HCV reinfection incidence rate was 4.13 per 100 per person‐years (py) (95% confidence interval [CI]: 3.45–4.81). The pooled incidence estimate among people who inject drugs (PWID) was 2.84 per 100 py (95% CI: 2.19–3.50), among men who have sex with men (MSM) 7.37 per 100 py (95% CI: 5.09–9.65) and among people in custodial settings 7.23 per 100 py (95% CI: 2.13–16.59). The pooled incidence estimate for studies reporting a testing interval of ≤6 months (4.26 per 100 py; 95% CI: 2.86–5.65) was higher than studies reporting testing intervals >6 months (5.19 per 100 py; 95% CI: 3.92–6.46).ConclusionsHCV reinfection incidence was highest in studies of MSM and did not appear to change with retesting interval. Shorter testing intervals are likely to identify more reinfections, help prevent onward transmission where treatment is available and enable progress towards global HCV elimination, but additional comparative studies are required.
Intrinsic cancer cell phosphoinositide 3‐kinase δ regulates fibrosis and vascular development in cholangiocarcinoma
Authors: Bou Malham Vanessa , Benzoubir Nassima , Vaquero Javier , Desterke Christophe , Agnetti Jean , Song Pei Xuan , Gonzalez‐Sanchez Ester , Arbelaiz Ander , Jacques Sophie , Di Valentin Emanuel , Rahmouni Souad , Tan Tuan Zea , Samuel Didier , Thiery Jean Paul , Sebagh Mylène , Fouassier Laura , Gassama‐Diagne Ama 
Published: October 2023

AbstractBackground & AimsThe class I‐ phosphatidylinositol‐3 kinases (PI3Ks) signalling is dysregulated in almost all human cancers whereas the isoform‐specific roles remain poorly investigated. We reported that the isoform δ (PI3Kδ) regulated epithelial cell polarity and plasticity and recent developments have heightened its role in hepatocellular carcinoma (HCC) and solid tumour progression. However, its role in cholangiocarcinoma (CCA) still lacks investigation.Approach & ResultsImmunohistochemical analyses of CCA samples reveal a high expression of PI3Kδ in the less differentiated CCA. The RT‐qPCR and immunoblot analyses performed on CCA cells stably overexpressing PI3Kδ using lentiviral construction reveal an increase of mesenchymal and stem cell markers and the pluripotency transcription factors. CCA cells stably overexpressing PI3Kδ cultured in 3D culture display a thick layer of ECM at the basement membrane and a wide single lumen compared to control cells. Similar data are observed in vivo, in xenografted tumours established with PI3Kδ‐overexpressing CCA cells in immunodeficient mice. The expression of mesenchymal and stemness genes also increases and tumour tissue displays necrosis and fibrosis, along with a prominent angiogenesis and lymphangiogenesis, as in mice liver of AAV8‐based‐PI3Kδ overexpression. These PI3Kδ‐mediated cell morphogenesis and stroma remodelling were dependent on TGFβ/Src/Notch signalling. Whole transcriptome analysis of PI3Kδ using the cancer cell line encyclopedia allows the classification of CCA cells according to cancer progression.ConclusionsOverall, our results support the critical role of PI3Kδ in the progression and aggressiveness of CCA via TGFβ/src/Notch‐dependent mechanisms and open new directions for the classification and treatment of CCA patients.