bluuebunny/crossref_metadata_2025_split huggingface dataset:
A common garden experiment supports a genetic component underlying the increased resilience of common cockle (Cerastoderma edule) to the parasite Marteilia cochillia
Authors: Villalba Antonio , Coimbra Raquel M. , Pampín Marina , Iglesias David , Costas Damián , Mariño Carlos , Blanco Andrés , Vera Manuel , Domínguez Marta , Cacabelos Eva , Abella Emilio , Incera Mónica , Otero Rosa Fernández , Martínez Paulino 
Published: October 2023

AbstractThe common cockle is a valuable bivalve species inhabiting the Atlantic European coasts. The parasite Marteilia cochillia has devastated cockle beds in the southern Galician (NW Spain) rias since 2012. Previous data suggested that cockles from Ría de Arousa acquired some resilience to this parasite through natural selection after consecutive annual marteiliosis outbreaks and candidate markers associated with marteiliosis resilience were identified using population genomics and transcriptomics approaches. Here, a common garden experiment was performed using a naïve stock (from Ría de Muros‐Noia) and an affected stock (from Ría de Arousa) to test this hypothesis. Breeders from both stocks were used to produce seed cohorts at hatchery, which were pre‐grown in a raft (outdoor nursery stage) and deployed in two shellfish beds affected by marteiliosis in Ría de Arousa (growing‐out stage). In both beds, the naïve stock showed high marteiliosis prevalence and was fully depleted in a short period, while the affected stock barely showed evidence of marteiliosis. A set of 45 SNPs putatively associated with marteiliosis resilience were fitted for MassARRAY genotyping to check their role in the differential resilience detected between both stocks. Though no significant differentiation was found between the naïve and the affected stocks with neutral markers, 28 SNPs showed significant divergence between them, suggesting that these SNPs were involved in directional selection during eight generations (to the most) of marteiliosis pressure (long‐term selection). Furthermore, signals of selection were also detected in the naïve stock along the marteiliosis outbreak in the growing‐out stage (short‐term selection) and six SNPs, all shared with the long‐term evaluation, showed consistent signals of differentiation according to the infection severity. Some of these SNPs were located within immune genes pertaining to families such as proteasome, ubiquitin, tumor necrosis factor, and glutathione S‐transferase. These resilience‐associated markers will be useful to recover cockle production in Galicia.
Pelvic flexure enterotomy in the horse: A review of current literature
Authors: Albanese Valeria , Straticò Paola , Munsterman Amelia 
Published: October 2023

SummaryEnterotomy of the pelvic flexure is commonly performed during colic surgery in the horse to empty the large colon of its contents. A two‐layer closure with absorbable suture material is recommended, but a consensus has not been established. Many different techniques have been described and tested to evaluate surgical time, luminal diameter reduction, bursting pressure and post‐operative complications including adhesion formation. The results of these studies vary within and between enterotomy techniques. The objective of this narrative review was to evaluate and summarise relevant published literature on pelvic flexure enterotomy techniques in the horse.
Geomicrobiology of the Rincon de Parangueo maar crater: Exploring the link between an evolving extreme environment and its potential metabolic diversity
Authors: Sánchez‐Sánchez Janet , Cerca Mariano , Moguel Bárbara B. , Muñoz‐Velasco Israel , Alcántara‐Hernández Rocío J. , Carreón‐Freyre Dora , Levresse Gilles 
Published: October 2023

Abstract

Rincon de Parangueo (RP) is a Quaternary maar crater located in the Michoacan‐Guanajuato Volcanic Field in central Mexico. Like other volcanic craters in the region, the central part was occupied by an endorheic lacustrine system. As a consequence of extensive groundwater extraction, the perennial lake started a gradual desiccation process and now the remnant ponds host a highly saline–alkaline ecosystem. The stratigraphic record indicates an exceptional and long‐term sedimentation of carbonate microbialites. Previous studies based on the 16S rRNA gene have shown that microbial communities have a widespread presence in the crater at the microbialites, the remaining saline ponds, superficial soils and below the surface. To understand the possible role of the microbial communities that generate these biogeological structures, novel analytical methods to resolve the amplicon datasets and their microbial metabolic potential were used.

We describe in detail the relationship between the microbial communities, the evolution of physicochemical parameters, and carbonate morphology, in four micro‐environments. A 16S rRNA gene sequencing dataset from previous publications was re‐analysed using an ASV‐based bioinformatic pipeline to identify new insights into the microbial assemblage. Finally, a taxon‐based metabolic profile was used to predict the potential metabolic contribution of the prokaryotic community.

Results indicated a refinement in terms of community composition using the ASV‐based bioinformatic methods. Functional profiling through 16S rRNA gene‐based analysis suggested metabolisms associated with carbonate precipitation, indicating a broad potential for microbially mediated carbonate precipitation (e.g., carbon fixation and photosynthesis). The possibility of biogenic methane gas production by methanogenic microorganisms was recognised, supporting the estimated flow of methane on the surface soils.

The lacustrine evolution over the last years and the extreme physicochemical characteristics of RP have an impact on the microbial community structure. Prokaryotic community and metabolic potential results from RP coincide with the diversity and abundance of microbial communities and functional metabolisms reported from other microbialite‐forming lakes along the Trans Mexican Volcanic Belt. The identification of important possible phylotypes involved in carbonate precipitation might be an important factor in considering RP microbialites as active calcifying entities.

These findings provide novel insights into the potential key role of metabolic pathways driving the process of carbonate precipitation inside RP and evidence of its importance as a microbial biodiversity hotspot in Mexico.
Effects of lipoprotein nanoparticles' composition and size on their internalization in plant and mammalian cells
Authors: Fukuda Ryosuke , Tani Misaki , Shibukawa Shiori , Nobeyama Tomohiro , Nomura Taiji , Kato Yasuo , Murakami Tatsuya 
Published: October 2023

AbstractThe internalization of engineered high‐density lipoprotein nanoparticles (engineered lipoproteins [eLPs]) with different lipid and protein compositions, zeta potentials, and/or sizes were analyzed in representative plant and mammalian cells. The impact of the addition of a cell‐penetrating peptide to eLPs on the internalization was very small in Bright Yellow (BY)‐2 protoplasts compared with HeLa cells. When eLPs were prepared with one of the abundant lipids in BY‐2 cells, digalactosyldiacylglycerol (DGDG) (eLP4), its internalization was dramatically increased only in HeLa cells. Such an increase in HeLa cells was also obtained for liposomes containing DGDG in a DGDG content‐dependent manner. Increasing the size and zeta potential of eLPs improved their internalization in both HeLa cells and in BY‐2 protoplasts but to quite varying degrees. Although eLPs tended to stay at the plasma membrane (PM) in BY‐2 protoplasts with much less internalization, the PM‐bound eLPs somehow promoted the internalization of coexisting nanobeads in cell culture media. These results provide fundamental insight into the future design of lipid nanoparticles for drug delivery in mammalian and plant cells.
We are not even allowed to call them patients anymore: Conceptions about person‐centred care
Authors: Määttä Sylvia , Björkman Ida 
Published: October 2023

AbstractAlthough the implementation of person‐centred care (PCC) has gained momentum in healthcare systems, it is not always obvious why it is an important objective in clinical settings and educational institutions that train future healthcare professionals. Various conceptions about PCC have flourished, such as that it only works in certain healthcare contexts, it is the same as the patient making all their own healthcare decisions, it is too demanding for most patients, the word ‘patient’ may no longer be used, the care provided is already person‐centred, and that there is simply not enough time and resources to achieve it. This viewpoint paper aims to problematize these conceptions and may be used for didactical and reflective exercises that support the transition towards more person‐centred healthcare.Patient or Public ContributionThe Person Council for patients and carers at the University of Gothenburg provided focus group input on and validated the occurrence of the conceptions discussed in the present paper. The person council consists of a group of persons with many years of personal experiences of health care, either as patients and/or relatives/carers. One member of the person council who is also a designer and artist created the illustration for the article.
The impact of social network embeddedness on mobile massively multiplayer online games play
Authors: Gong Mingchuan , Wagner Christian , Ali Ahsan 
Published: October 2023

AbstractSocial factors play a critical role in motivating player participation and commitment to online multiplayer games. Many popular mobile massively multiplayer online games (MMOGs) adopt social network embeddedness (SNE) functions to optimise players' social play experience. SNE changes the traditional pattern of MMOG social play by porting acquaintance relationships (e.g., Facebook friends) from social networking sites to the virtual game world. However, little understanding exists on how SNE impacts mobile MMOG players' game participation results such as play performance and play frequency. Drawing on the affordance framework and social capital literature, this research proposes a theoretical model that integrates the factors of SNE technology affordance (identity transparency and information transparency), players' social experience (social interaction, social support, shared vision, and social pressure), plus affordance effects (play performance and play frequency). The model was validated through a longitudinal field study, in which both subjective and objective data were collected from Game for Peace players. Our findings indicate that identity transparency and information transparency positively correlate with social interaction, social support, shared vision, and social pressure, which, taken together, significantly affect play frequency. The results also show that social interaction and shared vision positively impact players' play performance. The study enhances the theoretical understanding of social relationships in players' game participation results from the SNE aspect. Finally, we lend insights on how game operators can improve player game experience and stickiness.
Lethality Patterns in Multiple Suicide Attempts*
Authors: Worden J. William , Sterling‐Smith Robert S. 
Published: October 2023

ABSTRACT: There are few studies in the suicide literature where persons who make more than one suicide attempt are compared with persons who make only a single attempt. The present study investigated all patients (N = 347) who had come to Massachusetts General Hospital with a suicide attempt in 1965. In this group of patients, the 43 who had made multiple attempts were compared with a randomly selected group of 96 patients who had made only a single attempt.When compared to single attempters, multiple attempters made their initial attempt at an earlier age and at a lower lethal level (as measured by the Risk‐Rescue Rating), had a more intense history of prior psychiatric treatment, and received more psychiatric treatment subsequent to the first attempt. Multiple attempters did not differ from single attempters with regard to sex, race, religion, or marital status at the time of the first attempt.Within the group of multiple attempters two subgroups emerged: (a) those who were younger, whose initial lethality level was lower, and who had shorter time intervals between attempts; and (b) those who were older, had higher initial lethality levels, and had longer time periods between attempts. For both subgroups, receiving psychiatric treatment following the first attempt lowered the lethality of their second attempt.Multiple regression analysis was employed to predict the lethal level of a subsequent attempt, should such occur. This predictive equation and the descriptive differences cited above may have direct implications for the management of suicidal persons.
Systematic Observation of Crisis Center Telephone Interactions*
Authors: Powell Evan R. , Heaton Martha E. , Ashton Patricia T. 
Published: October 2023

ABSTRACTThe development of a systematic, objective method of categorizing helper‐helpee interactions as the first step in a predictive study is reported. Data on 100 initial suicidal telephone calls to three crisis intervention centers are generated from tapes of these calls. The 28,000 plus statements made in the calls are categorized into up to 8 categories in up to 11 levels. Descriptive data, including modal patterns, are presented. The importance of data rather than ratings is pointed out, and the Caller‐Worker Interaction Program is recommended as a research and monitoring tool with relevance to worker training.
Electromechanical properties of [001]‐textured Mn‐PMN‐PZT ceramics under hydrostatic pressure
Authors: Tang Mingyang , Hu Liqing , Wu Yue , Wang Yike , Liu Xin , Ren Xiaodan , Zheng Shuguang , Wang Sanhong , Xu Zhuo , Geng Liwei , Yan Yongke 
Published: October 2023

AbstractThe electromechanical properties of [001]‐textured Mn‐PMN‐PZT ceramics are characterized under a hydrostatic pressure of up to 400 MPa. The dielectric constant of textured ceramics increases first and then decreases as pressure increases. A comparison of the electromechanical constants sij, kij, and dij (i, j = 1, 3) obtained by the method of direct calculation and admittance fitting is displayed in this work. The results show that the two methods can obtain similar results at lower pressures. When the pressure is relatively high, the loss cannot be ignored, and the results of the two methods are quite different. The admittance fitting method gives a more accurate piezoelectric constant. Under 400 MPa, textured ceramics have k31 = 0.55, d31 = 321 pC/N, k33 = 0.89, and d33 = 702 pC/N, while random ceramics have k31 = 0.33, d31 = 92 pC/N, k33 = 0.71, and d33 = 230 pC/N. The large piezoelectric properties of textured ceramics under high pressure make them potential underwater transducers.
Opposing genetic patterns of range shifting temperate and tropical gastropods in an area undergoing tropicalisation
Authors: Zarzyczny Karolina M. , Hellberg Michael E. , Lugli Elena B. , MacLean Moira , Paz‐García David A. , Rius Marc , Ross Ethan G. , Treviño Balandra Erick X. , Vanstone James , Williams Suzanne T. , Fenberg Phillip B. 
Published: October 2023

AbstractAimThe poleward range expansion of tropical species, and range contraction of temperate species (known as tropicalisation) has mainly been studied from an ecological perspective, with little research on its genetic consequences. Here, we used distributional and genetic data to document the consequences of tropicalisation in rocky shore gastropods and assess more broadly the future implications of tropicalisation on phylogeographic patterns.LocationNineteen sampling sites along >3000 km of the eastern Pacific rocky intertidal zone, from the tip of the Baja California Peninsula to southern California.TaxonTemperate gastropods: Lottia conus, L. strigatella, Fissurella volcano and Tegula gallina.Tropical gastropods: Fissurella rubropicta, Nerita funiculata and N. scabricosta.MethodsWe determine historical and modern distributions of tropical and temperate species by combining historical records with current field surveys. Using a section of the cytochrome oxidase subunit I gene, we utilised comparative phylogeography, analysis of molecular variance, FST pairwise comparison, mismatch distributions of haplotype differences and neutrality tests to detect genetic signatures of tropicalisation and to better understand its consequences.ResultsWe identified range contractions in two temperate species and range expansion in all three tropical species. We detected genetic signatures of range expansion in the tropical species through unimodal distributions of pairwise haplotype differences and strongly negative values for the Fu and Li D and F* statistics. We found population subdivision and phylogeographic breaks in three temperate species, although the geographic location of the breaks differed among species.Main ConclusionsGenetic signatures and field surveys indicate recent range expansions in tropical species, supporting tropicalisation along the studied coastline. Conversely, we found phylogeographic breaks in temperate species, suggesting that tropicalisation may cause genetic erosion of evolutionary distinct lineages with range‐contraction. The different locations of the phylogeographic breaks among temperate species suggests that some barriers are species specific.
Ten recommendations for reducing the long‐term costs of conduct problems: A commentary on the economic analysis of Goulter et al. (2023)
Authors: Hawes David J. 
Published: October 2023

The economic analysis of Goulter et al. (2023) identifies the long‐term financial costs arising from conduct problems in the kindergarten period, including those associated with later criminal activity, lost offender productivity, victim costs and government and medical services. These costs are substantial and provide policymakers with a compelling argument for investing in early intervention and prevention of conduct problems. The ultimate goal of reducing the long‐term costs of early conduct problems is likely to be achieved only through the coordinated efforts of many stakeholders and diverse courses of action. Outlined here are 10 recommendations for investment that in my view would support progress towards this outcome.
Repair effect of Centella asiatica (L.) extract on damaged HaCaT cells studied by atomic force microscopy
Authors: Wan Linlin , Song Zhengxun , Wang Zuobin , Dong Jianjun , Chen Yujuan , Hu Jing 
Published: October 2023

AbstractPeople's choice of cosmetics is no longer just ‘Follow the trend’, but pays more attention to the ingredients of cosmetics, whether the ingredients of cosmetics are beneficial to people's skin health; therefore, more and more skin‐healthy ingredients have been discovered and used in cosmetics. In this work, atomic force microscope (AFM) is used to provide physical information about biomolecules and living cells; it brings us a new method of high‐precision physical measurement. Centella asiatica (L.) extract has the ability to promote skin wound healing, but its healing effect on damaged HaCaT cells needs to be investigated, which plays a key role in judging the effectiveness of skincare ingredients. The objective of this study was to explore the impact of Centella asiatica (L.) extract on ethanol‐damaged human immortalised epidermal HaCaT cells based on AFM. We established a model of cellular damage and evaluated cell viability using the MTT assay. The physical changes of cell height, roughness, adhesion and Young's modulus were measured by AFM. The findings indicated that the Centella asiatica (L.) extract had a good repair effect on injured HaCaT cells, and the optimal concentration was 75 μg/mL.
Unveiling the clinical and electrophysiological profile of CMTX6: Insights from two Brazilian families
Authors: Maciel Victor Augusto Zanesi , Maximiano‐Alves Gustavo , Frezatti Rodrigo Siqueira Soares , Alves Anna Letícia De Moraes , Andrade Bianca Mara Alves , Leal Rita De Cassia Carvalho , Tomaselli Pedro José , Reilly Mary M. , Marques Wilson 
Published: October 2023

AbstractBackground and AimsX‐linked Charcot–Marie–Tooth disease type 6 (CMTX6) is an extremely rare condition associated with mutations in the PDK3 gene. To date, only three families from different countries have been reported (Australia, South Korea, and Germany). In this study, we sought to provide a comprehensive clinical and electrophysiological characterization of two Brazilian families.MethodsWe conducted comprehensive clinical assessments, extensive electrophysiological evaluations, and performed whole‐exome sequencing in the probands to investigate the genetic basis of the disease.ResultsMales in the family carrying the Arg162His mutation displayed early‐onset motor and/or sensory axonal neuropathy, absence of tendon jerks, pes cavus, and frequently reported pain. Females in the same family exhibited a milder phenotype of the disease with later onset and some remained asymptomatic into their 50s. In the unrelated family with a single affected male, the clinical presentation was characterized by severe progressive sensorimotor polyneuropathy accompanied by neuropathic pain.InterpretationWe report two Brazilian families with CMTX6 including one harboring a previously unpublished variant in the PDK3 gene, which co‐segregates with the disease as expected in a X‐linked disease. Notably, the clinical presentations across the five families with available descriptions, including our study, share striking similarities. Furthermore, the proximity of the three reported mutations suggests potential functional similarities and common underlying mechanisms. This study contributes to the growing knowledge of CMTX6 and underscores the importance of international collaborations in studying rare genetic disorders.
Social Comparison Inside Business Groups and Strategic Change: Evidence from Group‐affiliated Chinese Firms
Authors: Ma Pengcheng , Cui Lin , Xu Dean 
Published: October 2023

AbstractThis study examines the effect of performance feedback on strategic change with a focus on internal social comparison in a business group context. We argue that group affiliates are more responsive to internal social comparison with group peers than to external social comparison with industry peers. However, the salience of internal social comparison is subject to institutional contingencies. We test these arguments using panel data from 1449 group affiliates in China during the period 2005–12. We find that internal social comparison has a greater effect on a group affiliate's strategic change than does external social comparison. Moreover, this effect differential is smaller in groups located in regions with more developed market institutions but larger in state‐owned groups and groups managed by internally promoted CEOs.