bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multibranched polymethacrylates by anionic grafting-from method via desilylation of poly(4-trimethylsilylmethylstyrene)
Authors: Hiraki Yujiro , Terasaki Masaya , Iwamura Takeru , Adachi Kaoru 
Published: January 2024

Abstract
A benzyltrimethylsilane (BnTMS)/potassium tert-butoxide (t-BuOK) initiator system for anionic polymerization was applied to poly(4-trimethylsilylmethylstyrene) (PTMSMS) in the presence of 1,1-diphenylethylene (DPE) to construct polyanion macroinitiators and polymerize methacrylate monomers therefrom. Using this method, desilylation of PTMSMS was quantitatively processed and methacrylate monomers were polymerized from the macroinitiator. The obtained multibranched polymers had narrow molecular weight distributions, indicating the well-controlled polymerization of the monomers from the macroinitiator. Therefore, it was demonstrated that PTMSMS can be a suitable precursor for the preparation of multibranched polymers. Additionally, a sequential addition of methacrylate monomers to the polyanion macroinitiator lead to a core-shell multibranched polymer with a narrow molecular weight distribution.
Oral Antibiotics for Treatment of Gram-Negative Bacteremia in Solid Organ Transplant Recipients: A Propensity Score Weighted Retrospective Observational Study
Authors: Nussbaum Eliezer Zachary , Koo Sophia , Kotton Camille N 
Published: January 2024

Abstract

Background
We assessed the safety and efficacy of oral antibiotic step-down therapy for uncomplicated gram-negative blood stream infections in solid-organ transplant recipients.

Methods
We identified all solid-organ transplant recipients within the Massachusetts General and Brigham and Women's Hospital systems from 2016 to 2021 with uncomplicated gram-negative bacteremia involving an organism susceptible to an acceptably bioavailable oral antibiotic agent. Using inverse probability of treatment-weighted models based on propensity scores adjusting for potential clinical confounders, we compared outcomes of those transitioned to oral antibiotics with those who continued intravenous (IV) therapy for the duration of treatment. Primary endpoints were mortality, bacteremia recurrence, and reinitiation of IV antibiotics. Secondary endpoints included length of stay, Clostridioides difficile infection, treatment-associated complications, and tunneled central venous catheter placement.

Results
A total of 120 bacteremia events from 107 patients met inclusion criteria in the oral group and 42 events from 40 patients in the IV group. There were no significant differences in mortality, bacteremia recurrence, or reinitiation of IV antibiotics between groups. Patients transitioned to oral antibiotics had an average length of stay that was 1.97 days shorter (95% confidence interval [CI], −.39 to 3.56 days; P = .005). Odds of developing C. difficile and other treatment-associated complications were 8.4 times higher (95% CI, 1.5–46.6; P = .015) and 6.4 times higher (95% CI, 1.9–20.9; P = .002), respectively, in the IV group. Fifty-five percent of patients in the IV group required tunneled catheter placement. There was no difference in treatment duration between groups.

Conclusions
Oral step-down therapy was effective and associated with fewer treatment-related adverse events.
Classical dance and Mediterranean imaginaries: Jia Ruskaja in Fascist Italy
Authors: Veroli Patrizia 
Published: January 2024

Abstract
Russian dancer and film actress Jia Ruskaja (Evgeniya Borisenko, 1902–70) forged a rapidly rising career in Italy as a dancer, teacher and company director during the thirties. The author of La danza come un modo di essere (1927) was a female education reformer, whose Milan dance schools were subsidized by the Fascist Regime. In 1940, she was entrusted with the directorship of the newly founded Royal School for Classical Dance. Though inspired by Isadora Duncan, in the early thirties, Ruskaja added ballet to her modern dance since she considered it crucial for the sound training of the body. According to her, the century-old codified system of ballet, once mingled with the ability to make artfully crafted movements inspired by classical imagery (‘plastique’) and rhythmic gymnastics, could transform into a new and organic physical practice, able to create a new beginning in dance. Was this so? Could her modernized ballet, imbued with the Mediterranean myth that inspired a number of artists in the twenties and thirties, convey such a modernist futural thrust, or was it rather held captive by centuries-old balletic values and culture?
Ancient tragedy, yet modern music: musical compositions in the classical performances (1921–39)
Authors: Casali Giovanna 
Published: January 2024

Abstract
This contribution aims to analyse the role of music in the revival of Greek theatre that characterized twentieth-century Italy. In particular, it explores and compares the music composed for INDA productions between 1921 and 1939. From the beginning of the festival, music occupied a crucial role, thanks to Ettore Romagnoli’s emphasis on the musical component in reviving ancient Greek drama. The years from 1921 to 1927 were characterized by the collaboration between Romagnoli and Giuseppe Mulè, who attempted to revive ancient Greek music according to modern criteria. By 1930, the festival, run by an entirely Fascist committee, had taken on a nationalistic character, and music was integral to this. Indeed, during these years (1930–39), music acquired a pivotal role in emphasizing the heroic instances of ancient drama that had become functional to the Regime’s policy. Moreover, it was considered indispensable for this increasingly mass-oriented type of performance.
The proarrhythmogenic role of autonomics and emerging neuromodulation approaches to prevent sudden death in cardiac ion channelopathies
Authors: Tonko Johanna B , Lambiase Pier D 
Published: January 2024

Abstract
Ventricular arrhythmias in cardiac channelopathies are linked to autonomic triggers, which are sub-optimally targeted in current management strategies. Improved molecular understanding of cardiac channelopathies and cellular autonomic signalling could refine autonomic therapies to target the specific signalling pathways relevant to the specific aetiologies as well as the central nervous system centres involved in the cardiac autonomic regulation.
This review summarizes key anatomical and physiological aspects of the cardiac autonomic nervous system and its impact on ventricular arrhythmias in primary inherited arrhythmia syndromes. Proarrhythmogenic autonomic effects and potential therapeutic targets in defined conditions including the Brugada syndrome, early repolarization syndrome, long QT syndrome, and catecholaminergic polymorphic ventricular tachycardia will be examined. Pharmacological and interventional neuromodulation options for these cardiac channelopathies are discussed.
Promising new targets for cardiac neuromodulation include inhibitory and excitatory G-protein coupled receptors, neuropeptides, chemorepellents/attractants as well as the vagal and sympathetic nuclei in the central nervous system. Novel therapeutic strategies utilizing invasive and non-invasive deep brain/brain stem stimulation as well as the rapidly growing field of chemo-, opto-, or sonogenetics allowing cell-specific targeting to reduce ventricular arrhythmias are presented.
Cardiac monoamine oxidase-A inhibition protects against catecholamine-induced ventricular arrhythmias via enhanced diastolic calcium control
Authors: Shi Qian , Malik Hamza , Crawford Rachel M , Streeter Jennifer , Wang Jinxi , Huo Ran , Shih Jean C , Chen Biyi , Hall Duane , Abel E Dale , Song Long-Sheng , Anderson Ethan J 
Published: January 2024

Abstract

Aims
A mechanistic link between depression and risk of arrhythmias could be attributed to altered catecholamine metabolism in the heart. Monoamine oxidase-A (MAO-A), a key enzyme involved in catecholamine metabolism and longstanding antidepressant target, is highly expressed in the myocardium. The present study aimed to elucidate the functional significance and underlying mechanisms of cardiac MAO-A in arrhythmogenesis.

Methods and results
Analysis of the TriNetX database revealed that depressed patients treated with MAO inhibitors had a lower risk of arrhythmias compared with those treated with selective serotonin reuptake inhibitors. This effect was phenocopied in mice with cardiomyocyte-specific MAO-A deficiency (cMAO-Adef), which showed a significant reduction in both incidence and duration of catecholamine stress-induced ventricular tachycardia compared with wild-type mice. Additionally, cMAO-Adef cardiomyocytes exhibited altered Ca2+ handling under catecholamine stimulation, with increased diastolic Ca2+ reuptake, reduced diastolic Ca2+ leak, and diminished systolic Ca2+ release. Mechanistically, cMAO-Adef hearts had reduced catecholamine levels under sympathetic stress, along with reduced levels of reactive oxygen species and protein carbonylation, leading to decreased oxidation of Type II PKA and CaMKII. These changes potentiated phospholamban (PLB) phosphorylation, thereby enhancing diastolic Ca2+ reuptake, while reducing ryanodine receptor 2 (RyR2) phosphorylation to decrease diastolic Ca2+ leak. Consequently, cMAO-Adef hearts exhibited lower diastolic Ca2+ levels and fewer arrhythmogenic Ca2+ waves during sympathetic overstimulation.

Conclusion
Cardiac MAO-A inhibition exerts an anti-arrhythmic effect by enhancing diastolic Ca2+ handling under catecholamine stress.
Evaluating the impact of light quality on plant–herbivore interactions using hemp as the model system
Authors: Pojmann-Ezeonyilo Eze , Langenhoven Petrus , Ingwell Laura L 
Published: January 2024

Abstract
Light-emitting diodes (LED) offer energy-efficient and customizable light sources that can be tailored to optimize plant chemistry and growth characteristics. Indoor cannabis production is the most energy-intensive crop in the United States and suffers from insect pest infestations including the cannabis aphid, Phorodon cannabis Passerini, which can negatively impact yield. Here we investigated the potential of light quality (color) to manage Cannabis sativa plant chemistry and cannabis aphids to increase crop quality. Cannabis was grown indoors under LED lighting systems where we manipulated the color spectrum. Within each light treatment, a subset of plants was exposed to aphid herbivory. Physical and chemical plant responses and aphid biology were measured. The interaction between light quality and herbivory drove the time to the first flower (cola) in our experimental plants. Light quality did not impact THC/CBD, but plants under increased blue light had higher bud yield than those grown under white light. The red–blue light treatment resulted in the tallest plants with the lowest leaf-stem dry mass and bud yield. Herbivory decreased bud yield and lowered the concentration of CBD/THC in buds. Lastly, light quality impacted the reproduction and mortality of the cannabis aphid. This study demonstrates the capacity of light quality to impact plant growth traits but offers no evidence for light quality impacting CBD/THC production in Cannabis. More importantly, herbivory resulting from aphid feeding was shown to decrease CBD and THC. Light quality impacted pest biology, supporting the potential use of light quality as a pest management tool.
Incorporating reproductive system history data into cardiovascular nursing research to advance women’s health
Authors: Roberts Davis Mary , Hiatt Shirin O , Gupta Nandita , Dieckmann Nathan F , Hansen Lissi , Denfeld Quin E 
Published: January 2024

Abstract
The lack of sex-specific variables, such as reproductive system history (RSH), in cardiovascular research studies is a missed opportunity to address the cardiovascular disease (CVD) burden, especially among women who face sex-specific risks of developing CVD. Collecting RSH data from women enrolled in research studies is an important step towards improving women’s cardiovascular health. In this paper, we describe two approaches to collecting RSH in CVD research: extracting RSH from the medical record and participant self-report of RSH. We provide specific examples from our own research and address common data management and statistical analysis problems when dealing with RSH data in research.
Digest: Mating systems, intragenomic conflict, and speciation
Authors: Roy Scott William 
Published: January 2024

Abstract
Conflict over the degree of maternal investment in an offspring can exist between an offspring’s maternally inherited and paternally inherited alleles. Such conflict is not expected under self-fertilization. A new study led by Rifkin and Ostevik suggests that divergence in the degree of conflict between closely related outcrossing and selfing species can lead to aberrant early development of hybrids in morning glories. This dynamic represents a potentially powerful driver of reproductive incompatibility and thus speciation.
Theoretical hypothesis in a direct electron transfer between non-interacting Fe-S proteins within an artificial fusion
Authors: Lim Jae Kyu 
Published: January 2024

Abstract
Reduction of CO2 to formate utilizing formate dehydrogenases (FDHs) has been attempted biologically and electrochemically. However, the conversion efficiency is very low due to the low energy potential of electron donors and/or electron competition with other electron acceptors. To overcome such a low conversion efficiency, I focused on a direct electron transfer between two unrelated redox enzymes for the efficient reduction of CO2 and utilized the quantum mechanical magnetic properties of the [Fe-S] ([iron-sulfur]) cluster to develop a novel electron path. Using this electron path, we connected non-interacting carbon monoxide dehydrogenase and FDH, constructing a synthetic carbon monoxide:formate oxidoreductase as a single functional enzyme complex in the previous study. Here, a theoretical hypothesis that can explain the direct electron transfer phenomenon based on the magnetic properties of the [Fe-S] cluster is proposed.
Defective Assistive Device Involvement in Older Adult Emergency Department Visits
Authors: Restrepo Felipe , Mali Namrata , Sands Laura P , Abrahams Alan , Goldberg David M , Gruss Richard , Zaman Nohel , Shields Wendy , Omaki Elise , Ehsani Johnathon , Ractham Peter , Kaewkitipong Laddawan 
Published: January 2024

Abstract

Background and Objectives
Many older adults adopt equipment to address physical limitations and reduce dependence on others to complete basic activities of daily living. Although a few prior studies have considered injuries associated with assistive devices for older adults, those studies focused on older adults’ health and functional risks for injury. There is limited analysis of older adult injuries involving defective or malfunctioning assistive devices.

Research Design and Methods
Data from this study are from the National Electronic Surveillance System All Injury Program which collected data on consumer product-related injuries from a probability sample of 66 hospital Emergency Departments across the United States. Data from 30 776 older adult Emergency Department (ED) injury narratives from 2016 to 2020 were coded according to the assistive device involved and whether malfunctioning led to the injury. The study team manually examined all narratives in which the assistive device was coded to have malfunctioned.

Results
A total of 10 974 older adult ED cases were treated for 12 488 injuries involving a defective device. Injuries included 4 212 head and neck injuries (eg, concussion), 4 317 trunk injuries (eg, hip fractures), and 3 959 arm or leg injuries (eg, leg fracture). Of these patients, 4 586 were admitted to a hospital ward for further evaluation and treatment. Seventy percent of these patients were injured while using a walker; in contrast, wheelchairs were implicated in only 4% of the above cases. Design flaws were identified in 8 158 cases and part breakage/decoupling incidents in 2 816 cases.

Discussion and Implications
Our findings provide evidence that assistive devices are actively involved in older adult injuries. Further research is needed to reduce injuries associated with assistive devices by educating patients and their careproviders about device use and assembly and developing effective methods for informing manufacturers about malfunctioning devices.
Transcriptome Dynamics and Cell Dialogs Between Oocytes and Granulosa Cells in Mouse Follicle Development
Authors: Liu Wenju , Chen Chuan , Gao Yawei , Cui Xinyu , Zhang Yuhan , Gu Liang , He Yuanlin , Li Jing , Gao Shaorong , Gao Rui , Jiang Cizhong 
Published: January 2024

Abstract
The development and maturation of follicles is a sophisticated and multistage process. The dynamic gene expression of oocytes and their surrounding somatic cells and the dialogs between these cells are critical to this process. In this study, we accurately classified the oocyte and follicle development into nine stages and profiled the gene expression of mouse oocytes and their surrounding granulosa cells and cumulus cells. The clustering of the transcriptomes showed the trajectories of two distinct development courses of oocytes and their surrounding somatic cells. Gene expression changes precipitously increased at Type 4 stage and drastically dropped afterward within both oocytes and granulosa cells. Moreover, the number of differentially expressed genes between oocytes and granulosa cells dramatically increased at Type 4 stage, most of which persistently passed on to the later stages. Strikingly, cell communications within and between oocytes and granulosa cells became active from Type 4 stage onward. Cell dialogs connected oocytes and granulosa cells in both unidirectional and bidirectional manners. TGFB2/3, TGFBR2/3, INHBA/B, and ACVR1/1B/2B of TGF-β signaling pathway functioned in the follicle development. NOTCH signaling pathway regulated the development of granulosa cells. Additionally, many maternally DNA methylation- or H3K27me3-imprinted genes remained active in granulosa cells but silent in oocytes during oogenesis. Collectively, Type 4 stage is the key turning point when significant transcription changes diverge the fate of oocytes and granulosa cells, and the cell dialogs become active to assure follicle development. These findings shed new insights on the transcriptome dynamics and cell dialogs facilitating the development and maturation of oocytes and follicles.
Molecular Evolution of Protein Sequences and Codon Usage in Monkeypox Viruses
Authors: Shan Ke-Jia , Wu Changcheng , Tang Xiaolu , Lu Roujian , Hu Yaling , Tan Wenjie , Lu Jian 
Published: January 2024

Abstract
The monkeypox virus (mpox virus, MPXV) epidemic in 2022 has posed a significant public health risk. Yet, the evolutionary principles of MPXV remain largely unknown. Here, we examined the evolutionary patterns of protein sequences and codon usage in MPXV. We first demonstrated the signal of positive selection in OPG027, specifically in the Clade I lineage of MPXV. Subsequently, we discovered accelerated protein sequence evolution over time in the variants responsible for the 2022 outbreak. Furthermore, we showed strong epistasis between amino acid substitutions located in different genes. The codon adaptation index (CAI) analysis revealed that MPXV genes tended to use more non-preferred codons compared to human genes, and the CAI decreased over time and diverged between clades, with Clade I &gt; IIa and IIb-A &gt; IIb-B. While the decrease in fatality rate among the three groups aligned with the CAI pattern, it remains unclear whether this correlation was coincidental or if the deoptimization of codon usage in MPXV led to a reduction in fatality rates. This study sheds new light on the mechanisms that govern the evolution of MPXV in human populations.
Global Marine Cold Seep Metagenomes Reveal Diversity of Taxonomy, Metabolic Function, and Natural Products
Authors: Yu Tao , Luo Yingfeng , Tan Xinyu , Zhao Dahe , Bi Xiaochun , Li Chenji , Zheng Yanning , Xiang Hua , Hu Songnian 
Published: January 2024

Abstract
Cold seeps in the deep sea are closely linked to energy exploration as well as global climate change. The alkane-dominated chemical energy-driven model makes cold seeps an oasis of deep-sea life, showcasing an unparalleled reservoir of microbial genetic diversity. Here, by analyzing 113 metagenomes collected from 14 global sites across 5 cold seep types, we present a comprehensive Cold Seep Microbiomic Database (CSMD) to archive the genomic and functional diversity of cold seep microbiomes. The CSMD includes over 49 million non-redundant genes and 3175 metagenome-assembled genomes, which represent 1895 species spanning 105 phyla. In addition, beta diversity analysis indicates that both the sampling site and cold seep type have a substantial impact on the prokaryotic microbiome community composition. Heterotrophic and anaerobic metabolisms are prevalent in microbial communities, accompanied by considerable mixotrophs and facultative anaerobes, highlighting the versatile metabolic potential in cold seeps. Furthermore, secondary metabolic gene cluster analysis indicates that at least 98.81% of the sequences potentially encode novel natural products, with ribosomally synthesized and post-translationally modified peptides being the predominant type widely distributed in archaea and bacteria. Overall, the CSMD represents a valuable resource that would enhance the understanding and utilization of global cold seep microbiomes.
DVsc: An Automated Framework for Efficiently Detecting Viral Infection from Single-cell Transcriptomics Data
Authors: Leng Fei , Mei Song , Zhou Xiaolin , Liu Xuanshi , Yuan Yefeng , Xu Wenjian , Hao Chongyi , Guo Ruolan , Hao Chanjuan , Li Wei , Zhang Peng 
Published: January 2024

Abstract
Single-cell RNA sequencing (scRNA-seq) has emerged as a valuable tool for studying cellular heterogeneity in various fields, particularly in virological research. By studying the viral and cellular transcriptomes, the dynamics of viral infection can be investigated at a single-cell resolution. However, limited studies have been conducted to investigate whether RNA transcripts from clinical samples contain substantial amounts of viral RNAs, and a specific computational framework for efficiently detecting viral reads based on scRNA-seq data has not been developed. Hence, we introduce DVsc, an open-source framework for precise quantitative analysis of viral infection from single-cell transcriptomics data. When applied to approximately 200 diverse clinical samples that were infected by more than 10 different viruses, DVsc demonstrated high accuracy in systematically detecting viral infection across a wide array of cell types. This innovative bioinformatics pipeline could be crucial for addressing the potential effects of surreptitiously invading viruses on certain illnesses, as well as for designing novel medicines to target viruses in specific host cell subsets and evaluating the efficacy of treatment. DVsc supports the FASTQ format as an input and is compatible with multiple single-cell sequencing platforms. Moreover, it could also be applied to sequences from bulk RNA sequencing data. DVsc is available at http://62.234.32.33:5000/DVsc.