bluuebunny/crossref_metadata_2025_split huggingface dataset:
Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage
Authors: Vorobey Nadiya , Kukol Kateryna , Kots Sergii , Pukhtaievych Petro , Patyka Volodymyr 
Published: December 2023

Background. With the increase in soybean cultivation areas, inoculants are becoming increasingly sought after. They are not only compatible with the original preparations for seed treatment of soybean but also offer the possibility of applying them for several days or even months before sowing into the soil.
Objective. The viability of new strains of nodule bacteria Bradyrhizobium japonicum (strain PC07 and strain B78) was investigated on the surface of soybean seeds treated with fungicides Fever and Maxim XL during the extended storage of inoculated seeds. Additionally, their ability for nitrogen fixation under symbiotic conditions was evaluated.
Methods. Сultivation, serial dilution method, determination of bacterial titer of inoculants, quantification of colony forming units (CFU), gas chromatography.
Results. In laboratory conditions, on the soybean seeds of Almaz and Vasylkivska varieties, the viability of the nodule bacteria B. japonicum PC07 and B78, which are more resistant to the active substances of these fungicides in pure culture, decreased. This reduction was dependent on the storage period, the fungicide used, and the strain of inoculant. One day after inoculation on the seeds without the use of fungicide, 68.6–75.4% CFU of the initial number was retained. Seeds treated with the fungicide Fever contained 36.5–38.3% CFU, and those treated with Maxim XL contained 26.2–27.0% CFU. The use of inoculants based on fungicide-resistant strains of B. japonicum PC07 and B78 also provided high cell viability – 2.41–2.8×106 (on the seeds without treatment), 1.40–1.70×106 (with Fever) and 0.8–1.17×106 (with Maxim XL) CFU/seed after 5 days of storage. The nitrogen-fixing activity of root nodules in soybean plants of Almaz and Vasylkivska varieties, whose seeds were treated with Fever, decreased on both varieties by 18.4–22.4% and 32.1–39.5%, respectively and for treatment with Maxim XL, the reduction was 24.5–33.7% and 47.7–75.2%, respectively, during the storage of seeds for 5 and 7 days before sowing, compared to control variants (seeds without fungicide treatment).
Conclusions. The utilization of fungicide-resistant strains of B. japonicum PC07 and B78 for bacterization of soybean seeds treated with fungicides Fever and Maxim XL provides a high inoculation titer during 5 days of storage. Viability of microbial cells on seeds treated with fungicides significantly diminishes during storage for more than 7–14 days. Insufficient titer of CFU of B. japonicum strains PC07 and B78 on the seed surface after 14 days of storage can considerably impede the efficacy of biopreparations. Therefore, it is crucial to seek substances that can enhance the resistance of rhizobia on seeds to the adverse effects of fungicide seed treatment, ensuring a longer period of their viability.
RUANG MENTAL DAN MODEL KOGNITIF IDEAL PEJABAT PEMERINTAH PADA HUMOR SATIRE #LORDLUHUT
Authors: Azizah Nurul 
Published: December 2023

<p>This research aims to (1) explain the relationship between the mental space of humor and #LordLuhut's satire; (2) describes the ideal concept of the idealized duties and authorities of government officials through the satire humor of #Lord Luhut. Citizen have the right to express their opinions and aspirations. There are many ways that can be done to convey the aspirations of a community to the government. One of them is by using social media to aspire their voice as a citizen. Criticism is conveyed through linguistic expressions on social media, one of which is manifested in satire humor. The data of this research is a form of humorous satire #LordLuhut which comes from various Twitter uploads. Data analysis was carried out qualitatively by using cognitive linguistic theories, such as mental space, profiling, prototypes and ideal cognitive models. The research results are presented with formal and informal methods. Several conclusions were obtained: (1) the existence of mental space in the expression of satire humor indicates that the expression is only imagination, not reality; (2) there is an ideal concept of a government official written by the author in the humorous satire #LordLuhut.</p><p> </p>
Partial Blue Light Blocking Glasses at Night Advanced Sleep Phase and Reduced Daytime Irritability but Did Not Alter Salivary Melatonin Secretion in Japanese Schoolchildren
Authors: Nishino Seiji , Maeda-Nishino Naoya , Yoshimoto Ryohei , Ono Taisuke , Chiba Shintaro 
Published: December 2023

Abstract
In modern society, delayed sleep patterns among schoolchildren present challenges to academic attendance and performance. The impact of nighttime light exposure, especially blue wavelength light, on sleep delay has long been acknowledged. We investigated the effects of using partial blue light blocking glasses (JINS Screen Lens Heavy [40% cut]) on salivary melatonin levels, sleep patterns, circadian phase, and daytime behavior in 39 male schoolchildren aged 10–12. Participants alternated between blue light blocking and standard clear lens glasses for three hours before bedtime, over two weeks in a crossover design, with a one-week washout interval. While blue light blocking glasses did not influence melatonin levels, they significantly advanced the sleep phase (bedtime: 22.03 ± 0.08h vs. 22.10 ± 0.1h, p < 0.05, sleep onset: 22.26 ± 0.08h vs. 22.36 ± 0.10h, p < 0.05). The effects were more pronounced in the second week and accompanied by improved irritability and reduced disruptive behavior. Blue light blocking glasses can advance sleep phase and improve behavior in schoolchildren, warranting further mechanistic investigation.
Enhancing Lead Ion Removal from Simulated Wastewater through Continuous Electrocoagulation Process: Investigating Operating Parameters and Adsorption Behavior
Authors: Ajjam Sata , Hlih Basheer , Alwan Hameed Hussein 
Published: December 2023

Abstract

Electrocoagulation, a promising method for the removal of contaminants from aqueous solutions, was investigated in this study. The effects of different operational parameters on the removal of lead ions were examined using a continuous electrocoagulation process in an 800 ml electrocoagulation cell. The experimental setup involved varying the hydraulic retention time (HRT) in intervals of 15, 25, 35, and 45 minutes, adjusting the applied voltage from 2 to 10 volts, manipulating the rotation speed at 0, 50, 100, and 150 rpm, and altering the distance between the electrodes at 2.3 and 2.7 cm. The initial concentration of lead ions in the aqueous solution was set at 200 mg Hg+2/L. The results revealed that an extended HRT significantly enhanced the removal efficiency of lead ions, with the maximum efficiency attained at a 45-minute HRT. Increasing the applied voltage up to 10 volts also improved the removal efficiency. However, as the distance between the electrodes increased, the removal efficiency decreased. Regarding the rotation speed, an increasing trend in removal efficiency was observed up to 100 rpm. Beyond this threshold, the removal efficiency gradually declined due to the destabilization of the formed flocs. Under optimal operating conditions, including a hydraulic retention time of 45 minutes, an applied voltage of 10 volts, a rotational velocity of 100 rpm, 2.3 cm between the electrodes, a lead concentration of 200 mg/l, and a pH of 7, a remarkable maximum removal efficiency of 97% was achieved. The electrodes utilized in the experiment were composed of aluminum/stainless steel (Al/St. St.).
The Combined Treatment with Ketogenic Diet and Metformin Slows Tumor Growth in Two Mouse Models of Triple Negative Breast Cancer
Authors: Schmidt Karen , Thatcher Amber , Grobe Albert , Hicks Linda , Gu Haiwei , Sears Dorothy D. , Ellies Lesley G. , Kalachev Leonid , Kroll Eugene 
Published: December 2023

Abstract

BACKGROUND
Many tumors contain hypoxic microenvironments caused by inefficient tumor vascularization. Hypoxic tumors have been shown to resist conventional cancer therapies. Hypoxic cancer cells rely on glucose to meet their energetic and anabolic needs to fuel uncontrolled proliferation and metastasis. This glucose dependency is linked to a metabolic shift in response to hypoxic conditions.
METHODS
To leverage the glucose dependency of hypoxic tumor cells, we assessed the effects of a controlled reduction in systemic glucose by combining dietary carbohydrate restriction, using a ketogenic diet, with gluconeogenesis inhibition, using metformin, on two mouse models of triple-negative breast cancer (TNBC).
RESULTS
We confirmed that MET − 1 breast cancer cells require abnormally high glucose concentrations to survive in a hypoxic environment in vitro. Then, we showed that, compared to a ketogenic diet or metformin alone, animals treated with the combination regimen showed significantly lower tumor burden, higher tumor latency and slower tumor growth. As a result, lowering systemic glucose by this combined dietary and pharmacologic approach improved overall survival in our mouse model by 31 days, which is approximately equivalent to 3 human years.
CONCLUSION
This is the first preclinical study to demonstrate that reducing systemic glucose by combining a ketogenic diet and metformin significantly inhibits tumor proliferation and increases overall survival. Our findings suggest a possible treatment for a broad range of hypoxic and glycolytic tumor types, one that can also augment existing treatment options to improve patient outcomes.
Quality of life among patients with Sickle Cell Disease and their parents in Democratic Republic of Congo, qualitative study
Authors: ZIGASHANE Adrien BURUME , ONGEZI Emmanuel LURHANGIRHE , NYANGUNIA Nash MWANZA , OKITOTSHO Stanislas WEMBONYAMA , KAMBALE Richard MBUSA 
Published: December 2023

Abstract

Background
Sickle cells disease (SCD) is one of the most prevalent hemoglobinopathies. In Democratic Republic of Congo (DRC) Sickle cell disease is a serious public health issue aggravated by a limited access to health and social support. health and social support. SCD has an effect on individual overall well-being and quality of life. It has a negative impact on a patient's social life, schoolwork, physical and mental health, and career. In some cases, those who are impacted may have mental health conditions including grief, fear about dying, and denial of their illness.
Method
This qualitative study set out to assess the quality of life of children with SCD and their parents attending clinic ‘’Petite Clinique amis des enfants’’. For this study, we used in-depth interview.
Results
12 in-depth interviews were conducted including 7 parents of children with SCD and 5 teenagers’ patients. Five major interview themes emerged: (1) discovery circumstances, (2) Experience of sickle cell patients and/or their parents, (3) Repercussions of the disease in the lives of sickle cell patients, (4) Perceptions in relation to the disease, (5) cares issues and needs.
Conclusion
The findings point out the significance of creating culturally and locally relevant interventions to enhance access to healthcare, lower social and cultural barriers, foster better understanding of the disease, and integrate a model of routine follow-up for sickle cell patients, either in other secondary structures, which implies its integration within the DRC's health system.
A Novel Electromechanical System for Guide Vane Adjustment in a Small Turbine Test Rig
Authors: Kumar Sandeep , Gandhi Bhupendra K 
Published: December 2023

Abstract
A cost-effective and reliable electromechanical system for guide vane (GV) control is designed and developed, which is easy to install and operate for micro hydro and model turbine test rigs. The electromechanical system can hold the GVs against the forces of the flowing fluid. The GV control mechanism contains a two-phase permanent magnet synchronous motor (PMSM), field-oriented control, and a feedback mechanism to control current and speed. The transfer functions, algorithm, and working of PMSM by supervisory control and data acquisition are integrated with drivers written in C++. The validation of the model and prototype shows good agreement with a maximum deviation of 2.14% in unit discharge values. The GV operating mechanism is used to run the turbine at design and off-design operating conditions, and the corresponding flow field of the draft tube is analysed. The GVO of 34˚ is identified as a BEP condition that matches with the prototype's BEP condition. The maximum pressure pulsations are observed at 22.10˚ GVO, 63.70% of the BEP condition. The rotating vortex rope formed shows a normalised frequency of 0.284 with the normalised pressure fluctuations amplitude of 2 to 3 times.
Multi-modality Multi-task Model for mRS Prediction using Diffusion-weighted Resonance Imaging
Authors: Park In-Seo , Kim Seongheon , Jang Jae-Won , Park Sang-Won , Yeo Na-Young , Seo Soo Young , Jeon Inyeop , Shin Seung-Ho , Kim Yoon , Choi Hyun-Soo , Kim Chulho 
Published: December 2023

Abstract

This study focuses on predicting the prognosis of acute ischemic stroke patients with focal neurologic symptoms using a combination of diffusion-weighted magnetic resonance imaging (DWI) and clinical information. The primary outcome is a poor functional outcome defined by a modified Rankin Scale (mRS) score of 3-6 after 3 months of stroke. Employing nnUnet for DWI lesion segmentation, the study utilizes both multi-task and multi-modality methodologies, integrating DWI and clinical data for prognosis prediction. Integrating the two modalities was shown to improve performance by 0.04 compared to using DWI only. The model achieves notable performance metrics, with a dice score of 0.7375 for lesion segmentation and an area under the curve of 0.8065 for mRS prediction. These results surpass existing scoring systems, showing a 0.16 improvement over the Totaled Health Risks in Vascular Events score. The study further employs grad-class activation maps to identify critical brain regions influencing mRS scores. Analysis of the feature map reveals the efficacy of the multi-tasking nnUnet in predicting poor outcomes, providing insights into the interplay between DWI and clinical data. In conclusion, the integrated approach demonstrates significant advancements in prognosis prediction for cerebral infarction patients, offering a superior alternative to current scoring systems.
Clinical outcome of extended curettage with postoperative denosumab administration for the treatment of Campanacci Grade III giant cell tumors of the extremities
Authors: Zhang Shuai , Zhao JiaQi , Song Lei 
Published: December 2023

Abstract
Background
Management of Campanacci Grade III giant cell tumors of the extremities is controversial because wide resection with reconstruction results in a poor functional outcome for most patients. Denosumab has been used before operation to enable curettage and surgical downstaging in patients with giant cell tumor of bone (GCTB), where joint preservation is difficult. However, owing to osteosclerosis of the lesion and difficulty to curet the lesion thoroughly, preoperative denosumab treatment increases the local recurrence rate. It is unclear whether postoperative treatment with denosumab facilitate the ability of a joint to be salvaged by intralesional surgery instead of resection. Therefore, we performed a retrospective study to investigate the local recurrence rate, joint preservation status, and functional outcomes after extended curettage and postoperative denosumab treatment in Campanacci Grade III giant cell tumors of the extremities.
Methods
We retrospectively reviewed 23 patients with Campanacci Grade III GCTB of the extremities in our hospital from January 2017 to June 2023, who underwent extended curettage and postoperative denosumab administration alone, without preoperative denosumab treatment. Patients were followed for adverse events of denosumab, surgical outcome, limb function of lesions, and local recurrence following extended curettage with postoperative denosumab .
Results
All incisions were healed without deep infection and internal fixation failure. The mean age of the patients at surgery was 36.6 years, and the mean followup was 35.8 months ( range, 6–72 months). There of the 23 patients had local recurrence years postoperatively. The recurrence rate is 13.0%. Two was treated with repeat intralesional surgery with no additional recurrence 2 years later, and another was treated with en bloc resection and reconstruction with vascularised fibular graft. One patient had progression to knee osteoarthritis without oral analgesics. No patient had pulmonary metastases develop and malignant transformation of GCTB. The mean Musculoskeletal Tumor Society functional scores at last followup were 27.3 of 30 (range,25–29). No serious adverse events were detected due to denosumab.
Conclusion
Our observations suggest extended curettage with postoperative denosumab administration is a reasonable option for Campanacci Grade III giant cell tumors of the extremities. Extended curettage with adjuvant denosumab therapy resulted in beneficial surgical downstaging, including either a less morbid surgical procedure or delayed en bloc resection. Maybe, resection should be considered when structural integrity cannot be regained after bone grafting or bone cement filling combined with intemal fixation.
Genome-wide identification of WRKY genes in soybean and functional analysis of GmWRKY162-GmGSL7c in resistance to soybean mosaic virus
Authors: Zhao Zhihua , Wang Rongna , Su Weihua , Sun Tianjie , Wei Fengju , Yu Zhouliang , Xiao Fuming , Yan Long , Yang Chunyan , Zhang Jie , Wang Dongmei 
Published: December 2023

Abstract
Background Soybean mosaic disease caused by soybean mosaic virus (SMV) is one of the most devastating and widespread diseases in soybean producing areas worldwide. The WRKY transcription factors (TFs) are widely involved in plant development and stress responses. However, the roles of GmWRKY TFs in resistance to SMV infection are largely unclear.Results Here, a total of 185 GmWRKYs were identified from soybean (Glycine max), and were clustered into 3 groups and 7 subgroups. The characteristics, such as gene structure, conserved motifs, cis-regulatory elements, and selective pressure, were systematically analyzed. Furthermore, 60 GmWRKY genes were differentially expressed during SMV infection according to the transcriptome data. Among these genes, the expression of GmWRKY162 decreased after imidazole treatment in the incompatibility combination in soybean varieties Jidou 7 with SMV strain N3, which was confirmed by RT‒qPCR. Remarkably, the silencing of GmWRKY162 reduced callose deposition and enhanced virus spread during SMV infection. Next, EMSA and ChIP‒qPCR revealed that GmWRKY162 could directly bind to the promoter of GmGSL7c, which was reported to be involved in callose synthesis in our previous study.Conclusion Our study revealed that GmWRKY162 play a positive role in increasing callose deposition and suppressing virus spread during SMV infection by targeting GmGSL7c, which providing valuable insights into the roles of WRKY family genes in response to SMV and guidance for future studies in understanding virus-resistance mechanisms in soybean.
Risk and protective factors in the development of subthreshold anxiety during early adolescence
Authors: Chen Keyin , Li Qian , Zhang Jiayuan , Niu Lijing , Dai Haowei , Peng Lanxin , Wang Xingqin , Ma Qing , Zhang Ruibin 
Published: December 2023

Abstract
Background
Subthreshold anxiety (STA) is a significant risk factor for developing anxiety disorders (AX), especially during adolescence. Identifying the risk and protective factors in early life is crucial for early prevention and intervention. However, there is limited research on this topic among early adolescents.
Methods
We studied 11876 early adolescents from the Adolescent Brain and Cognitive Development (ABCD) Study to investigate factors influencing the evolution of STA from ages 9–10 to 12–13. Outcomes included developing AX, persistent STA, and recovery from STA. 786 participants were identified as STA using Child Behavior Checklist (CBCL). We incorporated 31 diathesis-stress-related variables as predictors, encompassing demographics, mental and physical health, and environment, and employed logistic regression for modeling STA transitions.
Results
Compared to HCs, adolescents with STA have a 6.93 odds ratio (OR) for converting to AX in three years. Risk factors for conversion include psychiatric family history, behavior inhibition, depression, sluggish cognitive tempo, oppositional defiance, and sleep disturbance. Those maintaining STA experience fewer risk factors like behavioral inhibition, depression, somatic problems, sleep disturbance, and family conflict. Recovery from STA is challenging with psychiatric family history, prodromal psychosis, depression, and sleep disturbance. Sensation seeking and lack of premeditation are protective against STA or transitioning to AX.
Conclusions
Various modifiable and non-modifiable factors were identified for prognosis of STA in early adolescence. Managing sleep routines, encouraging sensation seeking, and reducing premeditation could help prevent adverse outcomes. By targeting these modifiable factors, it becomes feasible to intervene and prevent adolescents at high risk early in life.
The evolution of organic material on Asteroid 162173 Ryugu and its delivery to Earth
Authors: Changela Hitesh , Kebukawa Yoko , Petera Lukas , Ferus Martin , Chatzitheodoridis Elias , Nejdl Lukas , Nebel Roman , Protiva Vaclav , Krepelka Pavel , Moravkova Jana , Holbova Radka , Hlavenkova Zuzana , Šamořil Tomáš , Yamashita Shohei , Takahashi Yoshio , Yada Toru , Nakato Aiko , Sobotkova Katerina , Tesarova Hana , Zapotok Dave 
Published: December 2023

Abstract

The recent return of samples from carbonaceous asteroids provides first insight into early Solar System prebiotic evolution from known planetary bodies. Asteroid 162173 Ryugu samples are CI (Ivuna type), the most water- and organic-rich chondritic material, composing mostly of phyllosilicate. This phyllosilicate surrounds micron to submicron macromolecular organic particles - insoluble organic matter. Alteration on 162173Ryugu evolved insoluble organic material into organic particles aromatic-richer than what is found in the lesser altered carbonaceous chondrite matrices, consistent with CI and type 1 carbonaceous chondrites. Wider-spread diffuse organic material is also preserved within the phyllosilicate, aliphatic-, carboxylic-richer and aromatic-poorer than the organic particles. This diffuse organic material is probably a mixture of both acid insoluble and solvent soluble organic molecules. Carboxyl/aromatic ratios in the diffuse organic matter of Ryugu are also characteristic of CI and type 1 carbonaceous chondrites, indicating the role of aqueous alteration in its formation. Some organic particles additionally evolved to completely surround phyllosilicate itself, meaning that the soluble organic bearing diffuse organic matter is contained within some of the macromolecular organic particles by aqueous alteration on Ryugu. Earth therefore was, and currently is, delivered micron-sized polymeric organic objects encapsulating biologically relevant molecules.
Boundedness of fractional sublinear operators on weightedgrand Herz-Morrey spaces with Variable Exponents
Authors: Yang zhenzhen Yang , Zhang WanJing Zhang , Zhang Jing Zhang 
Published: December 2023

Abstract
In this paper, our goal is to further introduce the weighted grand variableHerz-Morrey spaces and establish the boundedness of fractional sublinear operatorsand their multilinear commutators on the weighted grand variable Herz-Morrey s-paces. The results are still new even in the unweighted setting, which generalizes theprevious work of Wang et al. [17] and Zhang et al. [19].