bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Method for Rapid Extraction of Fungal Genome for PCR
Authors: Tan Yiyang , Liu Shutong , Zhang Yanhua , Wang Depei , Xue Xianli 
Published: November 2023

Abstract
Objectives The present work aimed to develop a new method for rapid extraction of the fungal genome for Polymerase Chain Reaction (PCR).
Results(method) We used only 15 minutes to obtain an intact genome for PCR, due to cells were disrupted in the 0.5 mol/L NaOH solution. The quality and quantity of genomic DNA was verified by Nanodrop under OD260/OD280, even the density of dsDNA was measured. Firstly, we tried different lysis modes including enzymatic lysis and NaOH lysis. Finally the condition of NaOH cleavage method was selected and optimized for subsequent experiments. In conclusion, the optimal NaOH cleavage conditions were 100 μL of 0.5 mol/L NaOH to lysis the mycelium for 15 minutes at 25 ℃. The optimal growth time of mycelium was 48 hours, and the optimum amount to be used was 3.5 millimeter diameter. The method was applicable to different fungus and validation of multiple genes with diversable primers.
Conclusions Compared to traditional gene extraction methods, the new method was less time-consuming, safer, and simpler.. This method was so fast that it took only 15 minutes to complete the preparation of Polymerase Chain Reaction (PCR) reaction templates. The quality and quantity of extracted fungal genomes were confirmed at OD260/OD280 in the range of 1.8-1.9, and density in the range of 150-350 ng/μL. The new method was successfully applied to Aspergillus niger JH101, Saccharomyces Y3, Aspergillus niger ATCC1015, Aspergillus oryzae 3.042, Ustilago maydis UM1, Trichoderma reesei SUS, and even genetically engineered A. niger strains, and the success rate was remarkably high reaching to 89.2 %. Thus this technology can significantly increase the efficiency of basic molecular biology procedures.
Analysis of Prognostic Risk Factors and Establishment of a Prognostic Model for Liver Metastasis in Patients with Colon Cancer
Authors: Xiang Xingxing , Li Wei , Cao Yinghao , Liu Tao 
Published: November 2023

Abstract
Background
Colorectal cancer often metastasizes to the liver, which is associated with poor prognosis. The aim of this study was to establish an efficient nomogram model for predicting overall survival (OS) and disease-free-survival (DFS) in patients with colorectal cancer and liver metastasis.
Methods
We analyzed 421 patients diagnosed with colorectal cancer and liver metastasis at Tongji Medical College of Huazhong University of Science and Technology and Affiliated Union Hospital from January 2013 to December 2018. These patients were randomly assigned to training and validation cohorts. Single-factor and multivariate Cox regression analyses were performed to determine independent predictive risk factors and to construct nomograms for predicting OS and DFS. The performance of the nomograms was evaluated using calibration curves, area under the receiver operating characteristic curve (AUROC), and decision curve analysis (DCA).
Results
Tumor size, vascular tumor embolus, blood transfusion, number of liver metastases, number of sampled lymph nodes, staging, postoperative hospital stay, and carcinoembryonic antigen (CEA) were identified as independent predictive factors for liver metastasis. We developed a survival prediction model incorporating these eight prognostic factors. The Nomogram demonstrated good sensitivity in predicting 1-year, 3-year, and 5-year OS rates. In the training cohort, the AUROC for 1-year, 3-year, and 5-year OS was 0.793, 0.758, and 0.823, respectively. In the validation cohort, the respective AUROC values were 0.750, 0.704, and 0.822, respectively. Additionally, we constructed a column chart for patients' DFS based on histological subtype, number of sampled lymph nodes, vascular tumor embolus, number of liver metastases, perioperative transfusion, and CEA level. In the training cohort, the 1-year, 3-year, and 5-year DFS rates were 0.768, 0.716, and 0.803, respectively. In the validation cohort, the rates were 0.730, 0.839, and 0.838, respectively.
Conclusion
Based on clinical, pathological, and tumor biomarker characteristics, the newly constructed nomogram accurately predicted OS and DFS. This tool may be valuable for guiding clinical decision-making. In practice, individual patient data and analytical results may be used to develop personalized treatment plans that may improve prognosis and overall survival rates.
Touchscreen Communication (ToSCom): EQS Body Communication during Touch Sensing
Authors: Sen Shreyas , Datta Arunashish , Yang David , Maity Shovan 
Published: November 2023

Abstract
Touchscreens are a fundamental technology for human society providing the primary gateway for human-machine interaction. Today's touchscreens can only be used to detect touch and provide the location of the user's touch input but not to simultaneously communicate digital data during a touch event through the touchscreen. If communication through a touchscreen can be enabled, it promises deep societal impact by augmenting the most popular Human-Computer-Interaction interface with new possibilities such as a single application on the same device opening up personalized user-specific account data depending on the person interacting with the application. Leveraging significant advances in Electro-Quasistatic field based communication in the past decade, we propose and demonstrate Touchscreen Communication (ToSCom), a high-speed (> Mbps) simultaneous communication and touch sensing interface. We develop a low path loss channel across the entire touchscreen surface enabling 3 Mbps data rate communication with an average bit-error-rate of less than 5*10^(-7) through the touchscreen surface simultaneously during touch sensing. ToSCom enables a wide range of possibilities in day-to-day life like in wearable devices like transactions in a POS system, audio/image file transfer, and viewing personalized data in touchscreen kiosks.
Mood profile in men and women of all ages is improved by leisure-time physical activity rather than work-related physical activity
Authors: Skurvydas Albertas , Istomina Natalja , Dadeliene Ruta , Majauskiene Daiva , Strazdaite Emilija , Lisinskiene Ausra , Valanciene Dovile , Uspuriene Aiste Barbora , Sarkauskiene Asta 
Published: November 2023

Abstract
Background
The aim of our study was to determine how six mood indicators (vigour, fatigue, depression, anger, confusion, tension) depend on moderate to vigorous physical activity, walking time and sedentary time at work, after working hours and during leisure time, in men and women from different age groups.
Methods
A total of 1,140 individuals aged 18 to 64 years participated in the study. The participants were enrolled in this cross-sectional survey using a snowball sampling method. An online questionnaire was shared through popular social networks and emails within the period October 2019 to June 2020. Mood responses were assessed using The Brunel Mood Scale-LTU (BRUMS-LTU). Physical activity was assessed using the long International Physical Activity Questionnaire (IPAQ). Descriptive analysis, a two-way analysis of variance (ANOVA), and linear regression analysis were used to interpret the data.
Results
The survey results showed that vigour and fatigue correlated significantly only with leisure-time moderate to vigorous physical activity (two-way ANOVA for vigour: leisure-time moderate to vigorous physical activity effect: p < .0001, \({ŋ}_{\text{p}}^{2}\)= .12; gender effect: p < .0001, \({ŋ}_{\text{p}}^{2}\)=.09, ns; two-way ANOVA for fatigue: leisure-time moderate to vigorous physical activity effect: p < .0001, \({ŋ}_{\text{p}}^{2}\)=.09; gender effect: p < .0001, \({ŋ}_{\text{p}}^{2}\)=.091; ns.) The present results show a significant positive correlation between women and men moods and leisure-time moderate to vigorous physical activity, the length of time walking to work and back home, and negative correlation between moods and leisure-time sedentary behaviour. However, there was no significant correlation between moods and work-related moderate to vigorous physical activity and household moderate to vigorous physical activity, walking at work, and sitting duration at work.
Conclusions
According to the regression analysis, exercising men in all age groups moved the most (had a higher moderate to vigorous physical activity level) during leisure time, the highest work-related moderate to vigorous physical activity was observed in men and women with lower education, and the highest household moderate to vigorous physical activity was observed in older age men and women living in rural areas.
Association between Oral Frailty and Physical Frailty Subdomains in Chinese Community-Dwelling Older Adults:A Cross-sectional Study
Authors: Yan Jin , Fan Li , Lu Bingjie , Yang Cunmei , Xu Qiuli , Wu Bing , Zhang Tianyi , Bai Xiao , Chen Rongxia , Liu Jian , Zhu Zhihui , Di Ning , Hu Yixin 
Published: November 2023

Abstract
Methods This cross-sectional study was conducted in urban communities in Beijing from March 2022 to June 2023. We chose the Frailty Phenotype (FP) to assess physical frailty and Oral Frailty Index-8 to assess oral frailty. The analysis of factors affecting oral frailty was analyzed by multi-factorial logistic stepwise regression.Results 427 participants (147 women; mean age 81.81 ± 9.54 years) were enrolled in this study, 169 (39.58%) were non-oral frailty and 258(60.42%) were oral frailty. Visit a dental clinic less than annually (75.0%) and tooth loss(70.2%) are the most prevalent subdomains in oral frailty adults. After adjusting covariates, logistic regression analysis revealed that frailty phenotype (OR: 1.433, 95% CI: 1.150–1.785,p = 0.001)was associated with oral frailty. In the subdomains of physical frailty, exhaustion( OR = 2.363, 95% CI:1.302–4.289, p = 0.005 ) and slow gait speed (OR = 1.752, 95% CI:1.076–2.853, p = 0.024 ) were associated with oral frailty .Conclusion People with physical frailty should be more concerned about oral health issues, exhaustion and slow gait speed can be the sensitive indicators of oral frailty.
In Silico Predictive Homology Modeling of PKHD-1 Protein: A Comparative Study among Three Different Species
Authors: SB Arunannamalai 
Published: November 2023

Abstract
Background: The PKHD-1 (Polycystic Kidney and Hepatic Disease-1) gene encodes a crucial protein vital for renal and hepatic functions. Mutations in PKHD-1 result in Autosomal Recessive Polycystic Kidney Disease (ARPKD), a severe disorder in early infancy. Despite its significance, the structural information on PKHD-1 remains limited, with few low-resolution structures accessible. Homology Modeling was employed to generate structural models of PKHD-1 proteins from three species: Homo sapiens (Human), Mus musculus (Mouse), and Canis lupus familiaris (Dog). Various bioinformatics tools were utilized for analysis and validation.
Results: Structural models of PKHD-1 proteins from different species were generated using Homology Modeling and advanced bioinformatics tools, including SWISS-Model, ProtParam, GOR4, PROSA Web, ExPasy QMEANDisCo, and P2Rank. The primary structure, physicochemical properties, and secondary structure of PKHD-1 proteins were analyzed and validated. Binding pockets critical for understanding functional roles and potential therapeutic interventions were predicted using the P2Rank tool.
Conclusion: This study provides comprehensive structural insights into PKHD-1 proteins across multiple species. Rigorous validation of homology models through Z-Score analysis and QMEANDisCo Global Score ensures their reliability and accuracy. The identification of binding pockets offers potential targets for therapeutic interventions. Comparative analysis of PKHD-1 protein structures enhances understanding of evolutionary relationships and lays the foundation for future comparative functional studies. This research significantly contributes to structural biology and biomedical research, serving as a valuable resource for researchers investigating PKHD-1 function, disease mechanisms, and drug targeting strategies. The findings pave the way for exploring species-specific functions and adaptations of PKHD-1, fostering advancements in the understanding and treatment of ARPKD and related disorders.
3D-printed PCL framework assembling ECM-inspired multi-layer mineralized GO-Col-HAp microscaffold for in situ mandibular bone regeneration
Authors: Yang Yanqing , He Huan , Miao Fang , Yu Mingwei , Wu Xixi , Liu Yuanhang , Fu Jie , Chen Junwei , Ma Liya , Chen Xiangru , Peng Ximing , Zhen You , Zhou Chuchao 
Published: November 2023

Abstract
Background: In recent years, natural bone extracellular matrix (ECM)-inspired materials have found widespread application as scaffolds for bone tissue engineering. However, the challenge of creating scaffolds that mimic natural bone ECM's mechanical strength and hierarchical nano-micro-macro structures remains. The purposes of this study were to introduce an innovative bone ECM-inspired scaffold that integrates a 3D-printed framework with hydroxyapatite (HAp) mineralized graphene oxide-collagen (GO-Col) microscaffolds and find its application in the repair of mandibular bone defects.
Methods: Initially, a 3D-printed PCL scaffold was designed with cubic disks and square pores to mimic the macrostructure of bone ECM. Subsequently, we developed multi-layer mineralized GO-Col-HAp microscaffolds (MLM GCH) to simulate natural bone ECM's nano- and microstructural features. Systematic in vitro and in vivo experiments were introduced to evaluate the ECM-inspired structure of the scaffold and to explore its effect on cell proliferation and its ability to repair rat bone defects.
Results: The resultant MLM GCH/PCL composite scaffolds exhibited robust mechanical strength and ample assembly space. Moreover, the ECM-inspired MLM GCH microscaffolds displayed favorable attributes such as water absorption and retention and demonstrated promising cell adsorption and proliferation in vitro. The MLM GCH/PCL composite scaffolds exhibited successful bone regeneration within mandibular bone defects in vivo.
Conclusions: This study presents a well-conceived strategy for fabricating ECM-inspired scaffolds by integrating 3D-printed PCL frameworks with multilayer mineralized porous microscaffolds, enhancing cell proliferation and bone regeneration. This construction approach holds the potential for extension to various other biomaterial types.
Harnessing Strong Aromatic Conjugation in Low-Dimensional Perovskite Heterojunctions for High-Performance Photovoltaic Devices
Authors: Zhu Zonglong , Li Bo , Liu Qi , Gong Jianqiu , Li Shuai , Zhang Chunlei , Gao Danpeng , Chen Zhongwei , Li Zhen , Wu Xin , Zhao Dan , Yu Zexin , Li Xintong , Wang Yan , Lu Haipeng , Zeng Xiao Cheng 
Published: November 2023

Abstract
The combination of three-dimensional (3D) metal halide perovskite with low-dimensional (LD) perovskite in a heterojunction has shown significant potential for improving the performance of perovskite solar cells (PSCs). However, the large organic cations in LD perovskite often introduces carrier mobility anisotropy and impedes charge transport at the heterojunction interface, thereby limiting the overall efficiency of PSCs. In this study, we report a novel LD/3D perovskite heterojunction that introduces strong aromatic conjugated (SAC) LD perovskite materials at the 3D perovskite/electron extraction interface in p-i-n PSCs to reduce the electron transport resistance crossing the interface. SAC molecules possess a large π conjugated network through aromatic rings connected by conjugated bonds. This network results in continuous energy band orbits between the [Pb2I6]2− framework and the organic cation, thereby effectively suppressing interfacial non-radiative recombination and enhancing carrier extraction efficiency. As a result, the fabricated PSCs exhibit significantly improved efficiency, reaching up to 25.66% (certified 25.20%). Furthermore, these devices demonstrate exceptional stability, maintaining over 95% of the initial efficiency after 1200 hours and 1000 hours under ISOS-L-1I and ISOS-D-1 protocols, respectively. The chemistry design of SAC molecules in the LD/3D perovskite heterojunction represents a promising strategy for developing efficient and stable PSCs, paving the way for further advancements in the field of PSC technology.
Forced Intracellular Degradation of Xenoantigens: A Novel Modality for Cell-Based Cancer Immunotherapy
Authors: Rafei Moutih , Bikorimana Jean-Pierre , Farah Roudy , Abusarah Jamila , Erregragui Mohamed Ali , Gonçalves Marina , Talbot Sebastien , El-Hachem Nehme 
Published: November 2023

Abstract

Background: Antigen presentation is crucial in fighting cancer and infectious diseases. This process is however dependent on proteasomal degradation of captured or intracellular antigens. Given the recent leverage of mesenchymal stromal cells (MSCs) as a potent vaccination platform, we investigated whether forced degradation of an expressed experimental antigen fused to small degron sequences could prime potent antitumoral responses.
Methods: Retroviral vectors were used to gene-engineer murine MSCs. Besides comparing their in vitro antigen presentation capacity, qPCR and flow-cytometry were used to quantify gene expression and assess reactive oxygen (ROS) species production respectively. In addition, an immunopeptidome analysis was conducted to mine the peptide repertoire of engineered cells. Finally, the therapeutic potency of engineered MSCs was evaluated as a monotherapy or in combination with anti-PD-1 in syngeneic immunocompetent mice with a pre-established solid T-cell lymphoma.
Results: Despite similar transgene expression and absent changes in the expression of genes related to antigen presentation/loading or endoplasmic-reticulum stress, the MSC-UBvR-OVA group triggered potent T-cell activation due to enriched cell surface presentation of OVA-derived peptides. Nevertheless, the UBvR degron elicits mitochondrial ROS production, which appears to be important for efficient antigen processing. Administration of MSC-UBvR-OVA as a monotherapy to cancer-bearing mice controls tumor growth, an effect further enhanced using tranylcypromine-stimulated MSCs combined with the anti-PD-1 immune checkpoint.
Conclusions: Forced antigen degradation using the UBvR degron represents a plausible modality for the future development of MSC-based cancer vaccine expressing relevant tumor antigens.
Non-equivalent Atomic Vibrations at Interfaces in a Polar Superlattice
Authors: Hoglund Eric , Walker Harrison , Hussain Mohammad , Bao De-Liang , Ni Haoyang , Mamun Abdullah , Baxter Jefferey , Khan Asif , Pantelides Sokrates , Hopkins Patrick , Hachtel Jordan 
Published: November 2023

Abstract

In heterostructures made from polar materials, e.g., AlN-GaN-AlN, the non-equivalence of the two interfaces has long been recognized as a critical aspect of their electronic properties, in that they host different two-dimensional carrier gasses. Interfaces play as important a role in the vibrational properties of materials, where interface states enhance thermal conductivity and can generate unique infrared-optical activity. The non-equivalence of the corresponding interface atomic vibrations, however, has not been investigated so far due to a lack of experimental techniques with both high spatial and high spectral resolution. Herein we experimentally demonstrate the non-equivalence of AlN-(Al0.65Ga0.35)N and (Al0.65Ga0.35)N-AlN interface vibrations using monochromated electron energy-loss spectroscopy in the scanning transmission electron micro-scope (STEM-EELS) and employ density-functional-theory (DFT) calculations to gain insights in the origins of observations. We demonstrate that STEM-EELS possesses sensitivity to the dis-placement vector of the vibrational modes as well as the frequency, which enables direct mapping of the non-equivalent interface phonons between materials with different phonon polarizations in the interface direction. The physical origin of the non-equivalent interface behavior is then unraveled by understanding the localization and anisotropy of phonon displacements using DFT calculations. The results demonstrate the capacity to carefully assess the vibrational properties of com-plex heterostructures where interface states dominate the functional properties.
Prevalence of Radiographic Appendicular Osteoarthritis and Associated Clinical Signs in Young Dogs
Authors: Enomoto Masataka , de Castro Nicholas , Hash Jonathan , Thomson Andrea , Nakanishi-Hester Aoi , Perry Erin , Aker Savannah , Haupt Emily , Opperman Logan , Roe Simon , Cole Tracey , Thompson Nichola Archer , Innes John F , Lascelles B. Duncan X. 
Published: November 2023

Abstract
This study aimed to determine the prevalence of osteoarthritis (OA) and associated clinical signs in young dogs. Owners of dogs aged 8months-4years from a single practice, were contacted in random order, to participate in a general health screen. Clinical and orthopedic examinations were performed. Each joint was scored for pain reactions (0–4). Orthogonal radiographs of all joints were made under sedation. Each joint was scored for radiographic OA (rOA) severity on an 11-point scale. Clinical OA (cOA) was defined as an overlap of rOA and joint pain in ≥ 1 joint. Owners completed OA questionnaires. The owners of 123 dogs agreed to participate. Overall, 39.8% (49/123) of dogs had rOA in ≥ 1 joint, and 16.3% (20/123) or 23.6% (29/123) dogs had cOA, depending on the cut-off value of joint pain; moderate (2), or mild (1), respectively. Owners of dogs with cOA observed signs of impairment in approximately 30% of cases. Only 2 dogs with cOA were receiving OA pain management. The most commonly affected joints in descending order of frequency were elbow, hip, tarsus, and stifle. Radiographically visible OA is common in young dogs, and 40–60% of dogs with rOA had cOA. However, OA-pain appears underdiagnosed and undertreated in young dogs.
Social Vulnerability and Surgery Outcomes: A Cross-sectional Analysis
Authors: Abdelhack Mohamed , Tripathi Sandhya , Chen Yixin , Avidan Michael S. , King Christopher R. 
Published: November 2023

Abstract

Background
Post-operative complications present a challenge to the healthcare system due to the high unpredictability of their incidence. However, the socioeconomic factors that relate to postoperative complications are still unclear as they can be heterogeneous based on communities, types of surgical services, and sex and gender.
Methods
In this study, we conducted a large population cross-sectional analysis of social vulnerability and the odds of various post-surgical complications. We built statistical logistic regression models of postsurgical complications with social vulnerability index as the independent variable along with sex interaction.
Results
We found that social vulnerability was associated with abnormal heart rhythm with socioeconomic status and housing status being the main association factors. We also found associations of the interaction of social vulnerability and female sex with an increase in odds of heart attack and surgical wound infection.
Conclusions
Our results indicate that social vulnerability measures such as socioeconomic status and housing conditions could be related to health outcomes. This suggests that the domain of preventive medicine should place social vulnerability as a priority to achieve its goals.

The authors would like to note that Mohamed Abdelhack and Sandhya Tripathi contributed equally to this work.