bluuebunny/crossref_metadata_2025_split huggingface dataset:
No impact of time from diagnosis to treatment on survival in newly diagnosed AML treated with venetoclax-based regimens
Authors: Baden David , Zukunft Sven , Hernández Gema , Wolgast Nadine , Steinhäuser Sophie , Pohlmann Alexander , Schliemann Christoph , Mikesch Jan-Henrik , Steffen Björn , Sauer Tim , Hanoun Maher , Schäfer-Eckart Kerstin , Krause Stefan , Hänel Mathias , Einsele Hermann , Jost Edgar , Brümmendorf Tim , Scholl Sebastian , Hochhaus Andreas , Neubauer Andreas , Burchert Andreas , Kaufmann Martin , Niemann Dirk , Schaich Markus , Blau Wolfgang , Kiani Alexander , Görner Martin , Kaiser Ulrich , Kullmer Johannes , Weber Thomas , Berdel Wolfgang , Ehninger Gerhard , Müller-Tidow Carsten , Platzbecker Uwe , Serve Hubert , Bornhauser Martin , Röllig Christoph , Baldus Claudia , Fransecky Lars 
Published: January 2024

Abstract

In newly diagnosed acute myeloid leukemia, immediate initiation of treatment is standard of care. However, deferral of antileukemic therapy may be indicated to assess comorbidities or pre-therapeutic risk factors. We explored the impact of time from diagnosis to treatment on outcomes in newly diagnosed acute myeloid leukemia undergoing venetoclax-based therapy in two distinct cohorts.
By querying the Study Alliance Leukemia database and the health network TriNetX, we identified 138 and 717 patients respectively with an average age of 76 and 72 years who received venetoclax-based first-line therapy. When comparing patients who started treatment earlier or later than 10 days after initial diagnosis, no significant difference in median overall survival was observed - neither in the SAL cohort (7.7 vs. 9.6 months, p=.42) nor in the TriNetX cohort (7.5 vs. 7.2 months, p=.41). Similarly, severe infections, bleeding, and thromboembolic events were equally observed between early and later treatments, both in the overall patient groups and specific subgroups (age ≥75 years or leukocytes ≥20/nl).
This retrospective analysis indicates that postponing initiation of venetoclax-based therapy in newly diagnosed acute myeloid leukemia has no impact on survival or complication rates. However, both clinical assessment and individual preferences should be considered when deferring patients.
NephroPOC – Proenkephalin A 119-159 predicts acute kidney injury and 28-day mortality in patients with suspected organ dysfunction in the emergency department (ED): secondary analysis from the prospective observational LifePOC study
Authors: Neumann Caroline , de Sá Fabia Daniela Lobo , Hartmann Oliver , Lehmann Thomas , Kiehntopf Michael , Joannidis Michael , Bolanaki Myrto , Slagman Anna , Möckel Martin , Bauer Michael , Winning Johannes 
Published: January 2024

Abstract
Background:
Volume depletion, sepsis, major surgery, and nephrotoxins are the most common causes of acute kidney injury (AKI). The classical markers serum creatinine (Scr) and urine output (UO) for the evaluation of kidney function are of limited value in critical ill patients because they reflect an already existing organ dysfunction. Our hypothesis is that the measurement of the functional biomarker Proenkephalin A 119-159 (penKid), which is freely filtered in the glomerulus and is used as a marker for estimating the glomerular filtration rate, contributes to the early identification of patients with subclinical kidney damage.
Methods:
This was a secondary analysis of the prospective multicenter LifePOC study. We evaluated critically ill patients admitted to the emergency department (ED) with suspected organ dysfunction based on the risk-stratification tool qSOFA , who developed AKI, defined as Scr ≥0.3 mg/dl from baseline, within 72 hours of enrolment. The primary endpoint was evolving AKI after 48 h. AKI after 24 h, AKI after 72 h and 28-day mortality were defined as secondary endpoints.
Measurement and main results:
Within 48 h, 88 out of 453 patients (19.4%) developed AKI. Patients with AKI showed increased penKid levels at admission in comparison to patients without AKI (111.5 [73.0-247.5] pmol/l vs. 74.8 [47.2-120.4] pmol/l, p<0.001). PenKid was a superior predictor for AKI within 24, 48 and 72 h in comparison to Scr (all p<0.05), and the advantage increased the later the renal events occurred. Regarding 28-day mortality prediction, penKid also outperformed Scr (p<0.05). The observed superiority of penKid persisted if the recently proposed PENK-Crea formula to estimate the GFR was applied and compared to the latest CKD-EPI formula.
Conclusions:
Early measurement and the trajectory of penKid predicts early AKI and 28-day mortality in patients with suspected organ dysfunction in the ED superior compared to the classical marker Scr. The results indicate that the superiority is attributed to an earlier rise in penKid compared to Scr.
Trial registration: The trial was registered in the German Registry for Clinical Trials (DRKS00011188) on 20 October 2016.
Environment-Responsive Dopamine Nanoplatform for Tumor Synergistic Therapy
Authors: Deng Chunmin , Zhang Hao , Song Li 
Published: January 2024

Abstract
Nanoparticle-based photothermal therapy (PTT) has emerged as a promising approach in tumor treatment due to its high selectivity and low invasiveness. However, the penetration of near-infrared light (NIR) is limited, leading it fails to induce damage to the deep-seated tumor cells within the tumor tissue. Additionally, inefficient uptake of photothermal nanoparticles by tumor cells results in suboptimal outcomes for PTT. Based on the above-mentioned issues, this study utilized the adhesive properties of photothermal material, polydopamine (PDA), which can successfully load the photosensitizer indocyanine green (ICG) and chemotherapeutic drug doxorubicin (DOX) to achieve combined photothermal and chemotherapy treatment (PDA/DOX&ICG), aiming to compensate for the poor penetration of NIR in tumor tissues and the photothermal conversion performance of PDA. For the purpose of extending the blood circulation time of PDA/DOX&ICG nanoparticles, evading clearance by the body immune system and achieving targeted delivery to tumor tissues, a protective envelopment was created using erythrocyte membranes modified with folate acid (FA-EM). After reaching the tumor tissue, the obtained FA-EM@PDA/DOX&ICG nanoparticles can specific bind with folate acid receptors on the surface of tumor cells. This interaction facilitates improved uptake by tumor cells leading to the subsequent release of loaded DOX and ICG in response to the unique tumor microenvironment. DOX penetration ability can effectively compensate the limitation of NIR penetration at the tumor tissue. While ICG, as a typical photosensitizer, significantly enhances the photothermal conversion performance of FA-EM@PDA/DOX&ICG nanoparticles, thereby inducing tumor cells damage. In vitro and in vivo experimental results demonstrated that the coordinated NIR treatment with FA-EM@PDA/DOX&ICG not only effectively inhibits tumor growth but also exhibits superior biocompatibility, effectively mitigating DOX-induced tissue damage.
NLRX1 mediates the disruption of intestinal mucosal function caused by porcine astrovirus infection via the ERK/MLCK pathway
Authors: Tao Jie , Li Benqiang , Shi Ying , Cheng Jinghua , Tang Pan , Jiao Jiajie , Liu Huili 
Published: January 2024

Abstract

Considering the possible zoonotic nature of Porcine astrovirus (PAstV) and its frequent co-infection with porcine epidemic diarrhoea virus (PEDV), we investigated the impact of NLRX1 on the dysfunction of the intestinal mucosal barrier induced by PAstV infection. The study revealed a significant up-regulation of NLRX1 and LC3 II proteins in Caco-2 cells infected with PAstV. The reduction in PAstV titer occurred with the silencing of NLRX1 and the treatment of the autophagy inhibitor 3-MA. However, the use of 3-MA did not impact the expression of NLRX1. Additionally, PAstV infection triggered the activation of the ERK/MLC pathway and led to the down-regulation of tight junction proteins (Occludin and ZO-1) as well as MUC-2 expression. Silencing the expression of NLRX1 or treating it with 3-MA demonstrated the ability to inhibit MLC phosphorylation and elevate the levels of Occludin and ZO-1 proteins. Moreover, the inhibition of MLC phosphorylation was observed by treating the ERK inhibitor PD98059. Furthermore, adding the MLC inhibitor ML-7 mitigated the down-regulation of mucosa-related protein expression induced by PAstV infection. Nevertheless, the administration of PD98059 and ML-7 did not affect the expression of NLRX1. In summary, the data from this study confirm that NLRX1 plays a role in the disruption of intestinal mucosal function triggered by PAstV infection via the ERK/MLCK pathway. This information contributes to a better understanding of the pathogenesis of PAstV.
Disparities in Access to Hematopoietic Cell Transplant Persist at a Transplant Center
Authors: Shoag Jamie , Rotz* Seth , Hanna Rabi , Buhtoiarov Ilia , Dewey Elizabeth , Bruckman David , Hamilton Betty 
Published: January 2024

Abstract

Race, ethnicity, and socioeconomic status impact access to hematopoietic cell transplant (HCT). Whether differences in accessibility occur within HCT centers remains unknown.We performed a single-center retrospective review of 1,102 patients who underwent HCT consultation. We examined the association between race (Black vs. non-Black and White vs. non-White), ethnicity (Hispanic vs. non-Hispanic) and socioeconomic status (defined by zip code median household income quartiles) with receipt of HCT, time to HCT, and Psychosocial Assessment of Candidates for Transplantation (PACT) scores. Race and ethnicity were not associated with receipt of HCT (p>0.20 for all comparisons). Those living in higher income quartiles and those with private insurance were more likely to receive HCT (p=0.02 and p<0.001, respectively). Among HCT recipients, patients of White race had a shorter time to transplant than those of non-White race (p=0.0175). There was a strong association between lower PACT scores and poorer income quartiles (p<0.001). Socioeconomic status impacts receipt of HCT and PACT scores among patients evaluated at an HCT center. Race and ethnicity did not affect receipt of HCT. However, non-White patients waited longer from consultation to transplant. Further investigation as to whether the psychosocial eligibility evaluation impedes access to HCT in vulnerable populations is warranted.
Determinants of Human Papilloma Virus Vaccine Uptake Among Adolescent Girls at Selected Secondary Schools in Kibaha Town Council
Authors: NGAILO NEHEMIA , Mushi Lawrencia 
Published: January 2024

Abstract
Background: Human papilloma virus infection rates among girls aged 13 to 19 years are as high as 83%. Cervical cancer kills one woman every two minutes and affects approximately 570,000 women worldwide.This study investigated determinants of human papilloma virus vaccine uptake among adolescent girls at selected secondary schools in the Kibaha Town Council.
Methods: The research employed a mixed-method research approach. The study's sample size was 385 respondents, including 344 adolescent girl students, 40 parents/guardians, and 1 district immunization and vaccine officer. For quantitative data, the researcher collected the data and inserted them into Microsoft Excel for data sorting and cleaning. The cleaned data were then exported to SPSS version 26 to calculate descriptive data wich were represented in frequency and percentage. For qualitative data, the researcher used thematic content analysis.
Results: The results showed that only 47.92% of the total adolescent girls completed both doses of the HPV vaccine, while 52.08% of respondents did not receive the second dose. 66% of the respondents felt comfortable discussing the HPV vaccine. Most respondents (89.6%) believed that no negative health side effects were associated with HPV vaccine uptake.
Conclusion: There is a notable gap between awareness and action regarding HPV vaccination. The majority of respondents are aware of the importance of HPV vaccination in preventing cervical cancer, which indicates strong support for using the HPV vaccine. However, this positive attitude does not seem to translate into a high rate of girls actually completing both doses of the vaccine.
Genome-wide identification of the sorghum OVATE gene family and revelation of its expression characteristics in sorghum seeds and leaves
Authors: An Yanlin , Xia Xiaobo , Zhang Xiaoqin , Liu Li , Jiang Sixia , Jing Tingting , Zhang Feng 
Published: January 2024

Abstract
The OVATE gene family plays an important role in regulating the development of plant organs and resisting stress, but its expression characteristics and functions in sorghum have not been revealed. In this study, we identified 26 OVATE genes in the sorghum BTx623 genome, which were divided into four groups and distributed unevenly across 9 chromosomes. Evolutionary analysis showed that after differentiation between sorghum and Arabidopsis, the OVATE gene family may have experienced unique expansion events, and all OVATE family members were negatively selected. Transcriptome sequencing and RT-qPCR results showed that OVATE genes in sorghum showed diverse expression characteristics, such as gene SORBl_3001G468900 and SORBl_3009G173400 were significantly expressed in seeds, while SORBI_3005G042700 and SORBI_3002G417700 were only highly expressed in L1. Meantime, in the promoter region, a large number of hormone-associated cis-acting elements were identified, and these results suggest that members of the OVATE gene family may be involved in regulating specific development of sorghum leaves and seeds. This study improves the understanding of the OVATE gene family of sorghum and provides important clues for further exploration of the function of the OVATE gene family.
Patient-reported outcome measures in an outpatient parenteral antimicrobial therapy program– a prospective cohort study
Authors: Burch Andrea R. , Ledergerber Bruno , Ringer Martin , Zinkernagel Annelies S. , Eberhard Nadia , Kaelin Marisa B. , Hasse Barbara 
Published: January 2024

Abstract
Objectives Conventional perspectives that solely consider clinical outcomes and costs fail to acknowledge the patient's perspective. Outpatient Parenteral Antimicrobial Therapy (OPAT) has become a global standard in antimicrobial therapy. The objective of this prospective cohort study was to evaluate the influence of our local OPAT program on Health-Related Quality of Life (HrQoL) using Patient Reported Outcomes (PROs). This approach gave us an alternative perspective of high patient satisfaction further underlining the importance of OPAT for patients.
Methods Sociodemographic data, treatment details and outcomes were prospectively recorded for all patients enrolled in the University Hospital Zurich’s OPAT program. Eligible patients underwent interviews pre-discharge and 7-14 days post-discharge (in person or via phone) between October 2020 and September 2022. The assessments utilized a shortened, four-domain version of the validated Short Form-36 (SF-36) questionnaire, complemented by four additional questions gauging patient satisfaction.
Results Thirty-three patients participated in the study. Univariate analysis revealed substantial improvement in three of the four SF-36 domains. Specifically, participants reported enhanced role emotional (p=0.038), social functioning (p=0.003) and emotional well-being (p=0.003). Furthermore, 97% of patients would recommend OPAT to others.
Conclusion OPAT significantly impacts HRQoL and yields high patient satisfaction. Hospitals considering new OPAT programs should include both patient satisfaction and HRQoL impact in their argument repertoire for the introduction of OPAT.
Projected wind and waves around the Cuban archipelago using a multimodel ensemble
Authors: Mayo Axel Hidalgo , Llano Alejandro Vichot , Arenal Ida Mitrani 
Published: January 2024

Abstract
A statistical downscaling of wind and wave regimes is presented. The study is around the Cuban archipelago for the mid-term (2031–2060) and the long-term (2061–2090) with respect to the historical period 1976–2005. A multimodel ensemble of CMIP5 models under the RCP4.5 and the RCP8.5 scenarios is used. Projections of the wind and wave regimes are projected through the BIAS correction (delta and empirical quantile mapping), and multiple regression with a determination coefficient of 88.3%, a residual standard deviation of 0.11, and a square mean error of 0.29. The results obtained through the statistical downscaling do not show significant differences against previous results using dynamic downscaling over the region. According to the statistical downscaling, the mean annual wind speed and the wave height showed significant changes in the western part of the Cuban archipelago. The extreme indicators of climate change referred to by Hsig show similarity in the representation of the future Cuban marine climate, which would have the most accentuated changes on the north coast of the central and eastern regions.
Deep Convolution Neural Network Using TMC-2 DEM Images in Chandrayaan-2
Authors: Sinha Mimansa , Paul Sanchita , Ghosh Mili , Mohanty Sachi Nandan , Pattanayak Radha Mohan 
Published: January 2024

Abstract
The terrestrial planets, including the Moon, Earth, and Mars, have impact craters, contributing significantly to the solar system's complex geomorphology. However, conventional crater identification methods struggle with the accuracy, of their varied shapes, locations, and sizes. Our main aim is to locate lunar craters using Digital Elevation Model (DEM) images from Terrain Mapping Camera-2 (TMC-2) onboard the Chandrayaan-2 mission. Employing a crater-based U-Net model, CNN, Resnet18, and Image Net are utilized for weight training. The custom semantic segmentation network based on the U-Net model proves effective. The methodology involves Canny Edge Detection, pre-trained models, and bounding boxes for crater localization. Fully Convolutional Neural Networks (FCNN) and U-Net are applied to assess and recognize lunar craters in complex scenarios. The proposed model comprises a neural network, feature extractor, and optimization technique for lunar crater detection. The model achieves 80.95% accuracy using unannotated data and precision and recall are much better with annotated data and accuracy 86.91% in object detection with Chandrayaan-2's DEM photos. As we have only considered 2000 images as annotation is a time-consuming process, in the future we will use more image data sets so that our result is comparatively better for this.
Both heat stress and prey species affect Amblyseius orientalis performance
Authors: Zhou Xinyuan , Yan Hong , Hao Xuemin , Zhao Peipei , Sheng Fujing , Wang Endong , Xu Xuenong , Zhang Bo 
Published: January 2024

Abstract
Both temperature and prey closely impact the fitness and predation of natural enemies during biological control. Amblyseius orientalis (Ehara) (Acari: Phytoseiidae) is a native and effective predatory mite against spider mites and small sucking insects in many kinds of orchards and greenhouses, where high temperatures always occur during hot summer. However, the effects of heat stress and prey species on the fitness and predatory efficiency of A. orientalis have not been well understood. In current study, we selected two preys of Tetranychus urticae and Bemisia tabaci and two temperatures of 25℃ and 33℃ to explore the performances of A. orientalis under four combination treatments. The results showed that both temperature and prey significantly affect A. orientalis fitness. In detail, A. orientalis had the longest developmental duration of 7.63 days when feeding B. tabaci at 25℃, while the shortest development period was found in the group fed on T. urticae at 33℃. Heat stress and B. tabaci significantly decreased A. orientalis fecundity. High temperature significantly reduced the life span of A. orientalis, however, the longevity of A. orientalis feeding on T. urticae were significantly longer than those feeding on B. tabaci, regardless of temperature. Thus, our study assessing A. orientalis performances under different conditions can provide better biological control reference against pest mite and insect by native predatory mites in the fields.
Balanced Activation of Nrf-2/ARE Mediates Sulforaphane's Protective Effect on Keratoconus in Cell Mechanical Microenvironment
Authors: Liu Ruixing , Ma Ruojun , Yan Xiaoming 
Published: January 2024

Abstract
Keratoconus (KC) is a progressive degenerative disease that usually occurs bilaterally and is characterized by corneal thinning and the apical protrusion of the cornea. Oxidative stress is an indication of the accumulation of reactive oxygen species (ROS), and KC keratocytes exhibit increased ROS production compared with normal keratocytes. Therefore, oxidative stress in KC keratocytes may play a major role in the development and progression of KC. Here, we investigated the protective effect of the antioxidant sulforaphane (SF) using a hydrogel-simulated model of the cell mechanical microenvironment of KC. The stiffness of the KC matrix microenvironment in vitro was 16.70 kPa and the stiffness of the normal microenvironment was 34.88 kPa. Human keratocytes (HKs) were cultured for 24 h before observation or drug treatment with H2O2 in the presence or absence of SF. The levels of oxidative stress, nuclear factor E2-related factor 2 (Nrf-2) and antioxidant response element (ARE) were detected. The high-stress state of HKs in the KC cell mechanical microenvironment compensates for activation of the Nrf-2/ARE signaling pathway. H2O2 leads to increased oxidative stress and decreased levels of antioxidant proteins in KC. In summary, SF can reduce endogenous and exogenous oxidative stress and increase the antioxidant capacity of cells.
A hybrid approach for efficient outlier detection using supervised and unsupervised techniques
Authors: Jayaramulu C. , Venkateswarlu Bondu 
Published: January 2024

Abstract
Due to data imbalance and dimensionality, it is difficult to achieve optimal performance when detecting outliers in high-dimensional data. Numerous algorithms were developed in try to solve this issue. However, they have their advantages in identifying outliers from such data and are created using either supervised learning technique or unsupervised learning. While unsupervised learning techniques offer mechanisms for discovering and utilising complicated patterns, supervised learning techniques make use of training data. This paper's key premise is that you may "combine two methodologies to create a hybrid and reap the benefits of both worlds." We put forth a cutting-edge machine learning (ML) framework to evaluate this claim, combining supervised and unsupervised techniques for effective outlier detection. Additionally, we suggested an approach called the Multi-Model Approach for Outlier Detection (MMA-OD). The technique improves performance by utilising the advantages of both supervised and unsupervised learning models. Its strength is getting a better feature space. With several benchmark datasets, the suggested approach is assessed. According to the empirical findings, MMA-OD performs better than many other techniques.