bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitoguardin 1 and 2 promote granulosa cell proliferation by activating AKT and regulating the Hippo-YAP1 signaling pathway
Authors: Yan Ming-Qi , Zhu Bing-Hong , Liu Xiao-Hong , Yang Yu-Meng , Duan Xiu-Yun , Wang Yong , Sun Hui , Feng Mei , Li Tao , Liu Xiao-Man 
Published: November 2023

AbstractMitochondria have been identified to be involved in oxidative phosphorylation, lipid metabolism, cell death, and cell proliferation. Previous studies have demonstrated that mitoguardin (Miga), a mitochondrial protein that governs mitochondrial fusion, mitochondria-endoplasmic reticulum (ER) contacts, lipid formation, and autophagy, is crucial for ovarian endocrine and follicular development. Nevertheless, whether mammalian MIGA1 or MIGA2 (MIGA1,-2) regulates ovarian granulosa cell proliferation remains unclear. This study revealed that mammalian MIGA1,-2 promotes cell proliferation and regulates the phosphorylation and localization of Yes-associated protein 1 (YAP1) in ovarian granulosa cells. MIGA2 upregulation resulted in reduced YAP1 activity, while MIGA2 removal led to increased YAP1 activity. Further analysis indicated that MIGA1,-2 regulated YAP1 via the Hippo signaling pathway and regulated protein kinase B (AKT) activity in collaboration with YAP1. In addition, lysophosphatidic acid (LPA) regulated MIGA2 expression and AKT activity by activating YAP1. Briefly, we demonstrated that the mitochondrial MIGA1 and MIGA2, especially MIGA2, promoted cellular proliferation by activating AKT and regulating the Hippo/YAP1 signaling pathway in ovarian granulosa cells, which may contribute to the molecular pathogenesis of reproductive endocrine diseases, such as polycystic ovary syndrome (PCOS).
Universal selective transfer printing via micro-vacuum force
Authors: Park Sang Hyun , Kim Tae Jin , Lee Han Eol , Ma Boo Soo , Song Myoung , Kim Min Seo , Shin Jung Ho , Lee Seung Hyung , Lee Jae Hee , Kim Young Bin , Nam Ki Yun , Park Hong-Jin , Kim Taek-Soo , Lee Keon Jae 
Published: November 2023

AbstractTransfer printing of inorganic thin-film semiconductors has attracted considerable attention to realize high-performance soft electronics on unusual substrates. However, conventional transfer technologies including elastomeric transfer printing, laser-assisted transfer, and electrostatic transfer still have challenging issues such as stamp reusability, additional adhesives, and device damage. Here, a micro-vacuum assisted selective transfer is reported to assemble micro-sized inorganic semiconductors onto unconventional substrates. 20 μm-sized micro-hole arrays are formed via laser-induced etching technology on a glass substrate. The vacuum controllable module, consisting of a laser-drilled glass and hard-polydimethylsiloxane micro-channels, enables selective modulation of micro-vacuum suction force on microchip arrays. Ultrahigh adhesion switchability of 3.364 × 106, accomplished by pressure control during the micro-vacuum transfer procedure, facilitates the pick-up and release of thin-film semiconductors without additional adhesives and chip damage. Heterogeneous integration of III-V materials and silicon is demonstrated by assembling microchips with diverse shapes and sizes from different mother wafers on the same plane. Multiple selective transfers are implemented by independent pressure control of two separate vacuum channels with a high transfer yield of 98.06%. Finally, flexible micro light-emitting diodes and transistors with uniform electrical/optical properties are fabricated via micro-vacuum assisted selective transfer.
Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex
Authors: Pinotsis Nikos , Krüger Anna , Tomas Nicolas , Chatziefthymiou Spyros D. , Litz Claudia , Mortensen Simon Arnold , Daffé Mamadou , Marrakchi Hedia , Antranikian Garabed , Wilmanns Matthias 
Published: November 2023

AbstractThe identification and characterization of enzyme function is largely lacking behind the rapidly increasing availability of large numbers of sequences and associated high-resolution structures. This is often hampered by lack of knowledge on in vivo relevant substrates. Here, we present a case study of a high-resolution structure of an unusual orphan lipase in complex with an endogenous C18 monoacylglycerol ester reaction intermediate from the expression host, which is insoluble under aqueous conditions and thus not accessible for studies in solution. The data allowed its functional characterization as a prototypic long-chain monoacylglycerol lipase, which uses a minimal lid domain to position the substrate through a hydrophobic tunnel directly to the enzyme’s active site. Knowledge about the molecular details of the substrate binding site allowed us to modulate the enzymatic activity by adjusting protein/substrate interactions, demonstrating the potential of our findings for future biotechnology applications.
Metal-organic framework boosts heterogeneous electron donor–acceptor catalysis
Authors: Lin Jiaxin , Ouyang Jing , Liu Tianyu , Li Fengxing , Sung Herman Ho-Yung , Williams Ian , Quan Yangjian 
Published: November 2023

AbstractMetal-organic framework (MOF) is a class of porous materials providing an excellent platform for engineering heterogeneous catalysis. We herein report the design of MOF Zr-PZDB consisting of Zr6-clusters and PZDB (PZDB = 4,4’-(phenazine-5,10-diyl)dibenzoate) linkers, which served as the heterogeneous donor catalyst for enhanced electron donor–acceptor (EDA) photoactivation. The high local concentration of dihydrophenazine active centers in Zr-PZDB can promote the EDA interaction, therefore resulting in superior catalytic performance over homogeneous counterparts. The crowded environment of Zr-PZDB can protect the dihydrophenazine active center from being attacked by radical species. Zr-PZDB efficiently catalyzes the Minisci-type reaction of N-heterocycles with a series of C-H coupling partners, including ethers, alcohols, non-activated alkanes, amides, and aldehydes. Zr-PZDB also enables the coupling reaction of aryl sulfonium salts with heterocycles. The catalytic activity of Zr-PZDB extends to late-stage functionalization of bioactive and drug molecules, including Nikethamide, Admiral, and Myristyl Nicotinate. Systematical spectroscopy study and analysis support the EDA interaction between Zr-PZDB and pyridinium salt or aryl sulfonium salt, respectively. Photoactivation of the MOF-based EDA adduct triggers an intra-complex single electron transfer from donor to acceptor, giving open-shell radical species for cross-coupling reactions. This research represents the first example of MOF-enabled heterogeneous EDA photoactivation.
Antarctic daily mesoscale air temperature dataset derived from MODIS land and ice surface temperature
Authors: Nielsen Eva Bendix , Katurji Marwan , Zawar-Reza Peyman , Meyer Hanna 
Published: November 2023

AbstractKnowledge about local air temperature variations and extremes in Antarctica is of large interest to many polar disciplines such as climatology, glaciology, hydrology, and ecology and it is a key variable to understand climate change. Due to the remote and harsh conditions of Antarctica’s environment, the distribution of air temperature observations from Automatic Weather Stations is notably sparse across the region. Previous studies have shown that satellite-derived land and ice surface temperatures can be used as a suitable proxy for air temperature. Here, we developed a daily near-surface air temperature dataset, AntAir ICE for terrestrial Antarctica and the surrounding ice shelves by modelling air temperature from MODIS skin temperature for the period 2003 to 2021 using a linear model. AntAir ICE has a daily temporal resolution and a gridded spatial resolution of 1 km2. AntAir ICE has a higher accuracy in reproducing in-situ measured air temperature when compared with the well-established climate re-analysis model ERA5 and a higher spatial resolution which highlights its potential for monitoring temperature patterns in Antarctica.
Fractal basins as a mechanism for the nimble brain
Authors: Bollt Erik , Fish Jeremie , Kumar Anil , Roque dos Santos Edmilson , Laurienti Paul J. 
Published: November 2023

AbstractAn interesting feature of the brain is its ability to respond to disparate sensory signals from the environment in unique ways depending on the environmental context or current brain state. In dynamical systems, this is an example of multi-stability, the ability to switch between multiple stable states corresponding to specific patterns of brain activity/connectivity. In this article, we describe chimera states, which are patterns consisting of mixed synchrony and incoherence, in a brain-inspired dynamical systems model composed of a network with weak individual interactions and chaotic/periodic local dynamics. We illustrate the mechanism using synthetic time series interacting on a realistic anatomical brain network derived from human diffusion tensor imaging. We introduce the so-called vector pattern state (VPS) as an efficient way of identifying chimera states and mapping basin structures. Clustering similar VPSs for different initial conditions, we show that coexisting attractors of such states reveal intricately “mingled” fractal basin boundaries that are immediately reachable. This could explain the nimble brain’s ability to rapidly switch patterns between coexisting attractors.
Design and fabrication of a magnetic nanobiocomposite based on flaxseed mucilage hydrogel and silk fibroin for biomedical and in-vitro hyperthermia applications
Authors: Radinekiyan Fateme , Eivazzadeh-Keihan Reza , Naimi-Jamal Mohammad Reza , Aliabadi Hooman Aghamirza Moghim , Bani Milad Salimi , Shojaei Shirin , Maleki Ali 
Published: November 2023

AbstractIn this research work, a magnetic nanobiocomposite is designed and presented based on the extraction of flaxseed mucilage hydrogel, silk fibroin (SF), and Fe3O4 magnetic nanoparticles (Fe3O4 MNPs). The physiochemical features of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite are evaluated by FT-IR, EDX, FE-SEM, TEM, XRD, VSM, and TG technical analyses. In addition to chemical characterization, given its natural-based composition, the in-vitro cytotoxicity and hemolysis assays are studied and the results are considerable. Following the use of highest concentration of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite (1.75 mg/mL) and the cell viability percentage of two different cell lines including normal HEK293T cells (95.73%, 96.19%) and breast cancer BT549 cells (87.32%, 86.9%) in 2 and 3 days, it can be inferred that this magnetic nanobiocomposite is biocompatible with HEK293T cells and can inhibit the growth of BT549 cell lines. Besides, observing less than 5% of hemolytic effect can confirm its hemocompatibility. Furthermore, the high specific absorption rate value (107.8 W/g) at 200 kHz is generated by a determined concentration of this nanobiocomposite (1 mg/mL). According to these biological assays, this magnetic responsive cytocompatible composite can be contemplated as a high-potent substrate for further biomedical applications like magnetic hyperthermia treatment and tissue engineering.
VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance
Authors: Nan Xinsheng , Hardinge Patrick , Hoehn Sven , Dighe Shrinivas Nivrutti , Ukeri John , Pease Darius F. , Griffin Joshua , Warrington Jessica I. , Saud Zack , Hottinger Emma , Webster Gordon , Jones Davey , Kille Peter , Weightman Andrew , Stanton Richard , Castell Oliver K. , Murray James A. H. , Jurkowski Tomasz P. 
Published: November 2023

AbstractThe COVID-19 pandemic demonstrated the need for rapid molecular diagnostics. Vaccination programs can provide protection and facilitate the opening of society, but newly emergent and existing viral variants capable of evading the immune system endanger their efficacy. Effective surveillance for Variants of Concern (VOC) is therefore important. Rapid and specific molecular diagnostics can provide speed and coverage advantages compared to genomic sequencing alone, benefitting the public health response and facilitating VOC containment. Here we expand the recently developed SARS-CoV-2 CRISPR-Cas detection technology (SHERLOCK) to provide rapid and sensitive discrimination of SARS-CoV-2 VOCs that can be used at point of care, implemented in the pipelines of small or large testing facilities, and even determine the proportion of VOCs in pooled population-level wastewater samples. This technology complements sequencing efforts to allow facile and rapid identification of individuals infected with VOCs to help break infection chains. We show the optimisation of our VarLOCK assays (Variant-specific SHERLOCK) for multiple specific mutations in the S gene of SARS-CoV-2 and validation with samples from the Cardiff University Testing Service. We also show the applicability of VarLOCK to national wastewater surveillance of SARS-CoV-2 variants and the rapid adaptability of the technique for new and emerging VOCs.
Inverse association between adult height and diabetes risk in a cohort study of Chinese population
Authors: Li Xiaoli , Cheng Tiantian , Leng Lina , Song Guangyao , Ma Huijuan 
Published: November 2023

AbstractRecent studies linking adult height to diabetes risk remain controversial and few were from Asia. This study, therefore, aimed to explore the association of adult height with diabetes risk in a Chinese population. This retrospective cohort study was a secondary analysis of data from the DATADRYAD website, involving 211,172 non-diabetic individuals aged ≥ 20 years from the health screening program in China. Cox regression models were employed to evaluate hazard ratios (HRs) with 95% confidence interval (CI) of diabetes related to height. During an average 3.12-year follow-up, 4156 (1.97%) subjects reported developing diabetes. After adjusting for potential confounding factors, an inverse association of height with diabetes risk was observed among men and women [HR per 10 cm (95% CI), 0.78 (0.73–0.83) and 0.76 (0.68–0.86), respectively]. Moreover, subgroup analyses indicated the inverse association was only detected in individuals with aged < 70 years, fasting plasma glucose (FPG) < 6.1 mmol/L, and men with body mass index (BMI) < 28 kg/m2. In brief, height is inversely associated with diabetes risk in Chinese adults. Specifically, this association appears to be more pronounced in individuals with aged < 70 years, FPG < 6.1 mmol/L, and men with BMI < 28 kg/m2.
Robust in-vehicle heartbeat detection using multimodal signal fusion
Authors: Warnecke Joana M. , Lasenby Joan , Deserno Thomas M. 
Published: November 2023

AbstractA medical check-up during driving enables the early detection of diseases. Heartbeat irregularities indicate possible cardiovascular diseases, which can be determined with continuous health monitoring. Therefore, we develop a redundant sensor system based on electrocardiography (ECG) and photoplethysmography (PPG) sensors attached to the steering wheel, a red, green, and blue (RGB) camera behind the steering wheel. For the video, we integrate the face recognition engine SeetaFace to detect landmarks of face segments continuously. Based on the green channel, we derive colour changes and, subsequently, the heartbeat. We record the ECG, PPG, video, and reference ECG with body electrodes of 19 volunteers during different driving scenarios, each lasting 15 min: city, highway, and countryside. We combine early, signal-based late, and sensor-based late fusion with a hybrid convolutional neural network (CNN) and integrated majority voting to deliver the final heartbeats that we compare to the reference ECG. Based on the measured and the reference heartbeat positions, the usable time was 51.75%, 58.62%, and 55.96% for the driving scenarios city, highway, and countryside, respectively, with the hybrid algorithm and combination of ECG and PPG. In conclusion, the findings suggest that approximately half the driving time can be utilised for in-vehicle heartbeat monitoring.
Outbursts from an ice-marginal lake in Antarctica in 1969–1971 and 2017, revealed by aerial photographs and satellite data
Authors: Hata Shuntaro , Kawamata Moto , Doi Koichiro 
Published: November 2023

AbstractThe liquid water around the Antarctic Ice Sheet plays a key role in modulating both the vulnerability of ice shelves to hydrofracturing and ice discharge from outlet glaciers. Therefore, it needs to be adequately constrained for precise future projections of ice-mass loss and global sea-level rise. Although glacial lake outburst floods (GLOFs) pose one of the greatest risks in glacierized mountainous regions, any long-term monitoring of Antarctic ice-marginal lakes and their associated potential for GLOFs has been neglected until recently owing to the limited number of such events reported in Antarctica. Here we present direct evidence of repeated GLOFs from Lake Kaminotani-Ike, an ice-sheet-dammed lake in East Antarctica, via an analysis of historical aerial photographs and recent satellite data. Two GLOFs occurred in 1969–1971 and 2017, with discharge volumes of (8.6 ± 1.5) × 107 and (7.1 ± 0.4) × 107 m3, respectively, making them two of the largest GLOFs in Antarctica. A southerly oceanward pathway beneath the ice sheet is the most likely drainage route of these GLOF events based on the available surface- and bed-elevation datasets. Furthermore, the 2017 event occurred during the austral winter, thereby implying the possibility of year-round active subglacial networks in Antarctica. Our results highlight that studies on Antarctic ice-marginal lakes provide an opportunity to better understand Antarctic hydrological processes and emphasize the need for both detailed monitoring of ice-marginal lakes and detailed surveying of the subglacial environments of the Antarctic Ice Sheet.
EpCAM-positive circulating tumor cells and serum AFP levels predict outcome after curative resection of hepatocellular carcinoma
Authors: Kocheise Lorenz , Schoenlein Martin , Behrends Berit , Joerg Vincent , Casar Christian , Fruendt Thorben W. , Renné Thomas , Heumann Asmus , Li Jun , Huber Samuel , Lohse Ansgar W. , Pantel Klaus , Riethdorf Sabine , Wege Henning , Schulze Kornelius , von Felden Johann 
Published: November 2023

AbstractHepatocellular carcinoma (HCC) has high recurrence rates exceeding 50% despite curative resection. The serum biomarker alpha-fetoprotein (AFP) is a well-known prognostic marker for HCC. EpCAM-positive circulating tumor cells (CTC) have a high predictive value for early HCC recurrence after curatively intended resection, most likely indicating micro-metastases at the time of resection. However, sensitivity remains low. The objective of this study was to evaluate a composite test comprising both CTC and AFP to identify patients at high risk for early HCC recurrence. We prospectively enrolled 58 patients undergoing curative intended resection for HCC at a tertiary referral center. Blood specimens were obtained prior to resection and analyzed for EpCAM-positive CTC and serum AFP levels. A positive result was defined as either detection of CTC or AFP levels ≥ 400 ng/ml. Eight patients tested positive for CTC, seven for AFP, and two for both markers. A positive composite test was significantly associated with shorter early recurrence-free survival (5 vs. 16 months, p = 0.005), time to recurrence (5 vs. 16 months, p = 0.011), and overall survival (37 vs. not reached, p = 0.034). Combining CTC and AFP identified patients with poor outcome after surgical resection, for whom adjuvant or neoadjuvant therapies may be particularly desirable.
Metal surface defect detection based on improved YOLOv5
Authors: Zhou Chuande , Lu Zhenyu , Lv Zhongliang , Meng Minghui , Tan Yonghu , Xia Kewen , Liu Kang , Zuo Hailun 
Published: November 2023

AbstractDuring the production of metal material, various complex defects may come into being on the surface, together with large amount of background texture information, causing false or missing detection in the process of small defect detection. To resolve those problems, this paper introduces a new model which combines the advantages of CSPlayer module and Global Attention Enhancement Mechanism based on the YOLOv5s model. First of all, we replace C3 module with CSPlayer module to augment the neural network model, so as to improve its flexibility and adaptability. Then, we introduce the Global Attention Mechanism (GAM) and build the generalized additive model. In the meanwhile, the attention weights of all dimensions are weighted and averaged as output to promote the detection speed and accuracy. The results of the experiment in which the GC10-DET augmented dataset is involved, show that the improved algorithm model performs better than YOLOv5s in precision, mAP@0.5 and mAP@0.5: 0.95 by 5.3%, 1.4% and 1.7% respectively, and it also has a higher reasoning speed.
Expression prevalence and dynamics of GPCR somatostatin receptors 2 and 3 as cancer biomarkers beyond NET: a paired immunohistochemistry approach
Authors: Oron-Herman Mor , Kirmayer David , Lupp Amelie , Schulz Stefan , Kostenich Genady , Afargan Michel 
Published: November 2023

AbstractSomatostatin receptors are clinically validated GPCR biomarkers for diagnosis and treatment of various neuroendocrine tumors (NET). Among the five somatostatin receptors, SST2 and SST3 are associated with apoptosis and cell cycle arrest, making these receptor subtypes better differentiated targets in precision oncology. In this study we performed immunohistochemistry of paired tissue microarrays containing 1125 cores, representing 43 tumor types, each stained for SST2 and SST3. A 12-point immunoreactive scoring (IRS) range was used for interpretation of the staining results. We analyzed the results twice, using the conventional positivity IRS cutoffs ≥ 3 and more stringent ≥ 6. Evaluation of receptors expression dynamics was performed for tumor-nodes-metastases (TNM) defined subgroups (ovarian and hepatocellular adenocarcinomas) as a function of their tumor stage. Our results indicate that two-thirds of tested cores exhibit clinically significant expression of at least SST2 or SST3 (IRS ≥ 6). The expression prevalence of both receptors tends to decline with tumor progression. However, an unexpected upregulation of both SST2 and SST3 reemerged in metastases suggesting conserved receptors genetic potential during tumor life cycle. We suggest that SST2 and SST3 should be further explored as potential biomarkers and therapeutic tools for maximizing the efficiency of somatostatin-based precision oncology of solid tumors beyond NET.