bluuebunny/crossref_metadata_2025_split huggingface dataset:
N30 Development and implementation of a microlearning programme for patients with Inflammatory Bowel Disease. The ADEII project
Authors: Sanromán L , De Castro Parga M L , Figueira M , Hernández V , Martinez.Cadilla J , Rodriguez-Prada I 
Published: January 2024

Abstract

Background
Information and communication technologies (ICTs) represent a novel approach to chronic disease management. However, their use in Inflammatory Bowel Disease (IBD) is lacking, as no specific tools are available. Microlearning is a pedagogical concept that facilitates learning by breaking down concepts into micro-contents, which can be easily integrated into everyday activities. We aimed to develop a microlearning programme for IBD patients and implement it in clinical practice.

Methods
Prospective cohort study in patients with IBD (18 to 70 years old) who underwent a microlearning programme over a 2-month period, Four-minute videos recorded by IBD unit´s own staff, and organised in individualised pathways.were sent via mobile phone. The interaction with the user was maintained through a Telegram bot that uses methods from the Django REST API for content sequencing. The basic sequencing consists of a video followed by 4 quiz questions. Depending on the answers, the user receives more questions, additional content on the same topic or moves on to the next video. We conducted a final survey about the information shown in the videos.
All the contents can be found on the website: https://adeii.webs.uvigo.es/ This QR links to the promotional video of the ADEII project (Figure 1).

Results
We recorded 24 videos about IBD and treatment and another 2 about patients´personal experiences. Between 1 June and 31 October 2022, 1226 videos were sent to 200 IBD patients: 103 females (51.5%), mean age 46.1 (13) years, 105 ulcerative colitis (52.5%), and time from IBD diagnosis 13 (10) years. (Table 1). All patients received by their smart-phone the 4-video main pack, consisting of 2 videos with information about their disease (ulcerative colitis or Crohn's disease), 1 video on adherence and another one on corticosteroids.
To these we added as many videos as the number of drugs that each patient used to control IBD. Mean number of videos per patient was 6 (1), range (5-9) and their distribution is shown in Figure 1. Seven patients declined to participate in this course because of fear of information and one left the study. Altogether 156 patients (78%) attended this microlearning programme, 117 fully (58,5%). A telephone contact, planned to solve technical incidents or doubts, rose the attendants to 170 (90%), 152 (76%) fully. After the course, 72,5% of the participants (145) answered correctly more than 83% of the questions based on the IBD information included in this programme.

Conclusion
A microlearning ICT-based course was widely accepted by IBD patients, and can increase the knowledge about their disease. However there are acceptance and technological barriers that must be taken into account when approaching it.
ECCO Fellowship Effect of diet on disease development and faecal microbiota transplantation efficacy in humanized SAMP/Yit and DSS mice models
Authors: Deleu Sara , Scaldaferri Franco 
Published: January 2024

Abstract

Background and Aims
The gut microbiome and diet have been shown to be associated with IBD pathogenesis, and to be implicated as triggers in disease development. Increasing evidence shows that dysbiosis and Western diets may drive and sustain intestinal inflammation. Therefore, faecal microbiota transplantation (FMT) has been hypothesized to restore a balanced microbiome, leading to reduced inflammation and improved intestinal function.
The mechanisms by which FMT exerts its therapeutic effects in IBD are not fully understood. Yet, FMT has shown promising effects in controlling disease in randomized controlled studies in UC. In contrast, data on FMT in CD are scarce and worsening of disease has even been described (Vermeire et al., 2016). Unfortunately, up to day, this different effect of FMT in UC versus CD remains unexplained.
Therefore, the aim of this project is to evaluate the effect of FMT in a preclinical model of CD, and compare it to a UC-model and study the effect of ultra-processed Western diets.

Methods
The mice models which will be used include the acute DSS-induced colitis model for UC and the spontaneous terminal ileitis (SAMP/Yit) model for CD. Upon humanization of the mice with UC or CD patient’s stool, mice will be randomized to dietary interventions being a Western diet, a regular diet, or a Mediterranean diet. Subsequently, the mice will be randomized to receive either autologous (patient) FMT or one out of four healthy donor FMTs possessing different enterotypes.

Anticipated Impact
By evaluating, clinical endpoints, as well as microbiota and transcriptomic endpoints, this project will provide insights in the differences between FMT success in UC and CD, as well as in the importance of donor selection (enterotype matching) and additional effect of dietary interventions on inflammation. Herewith predicting that this project will aid to bring microbiota-targeted treatment strategies to the IBD clinic.
D-ECCO Grant Dietary patterns during early life and risk of inflammatory bowel disease
Authors: Brusco De Freitas M , Anneberg O M , Jess T 
Published: January 2024

Abstract

Background and Aims
Diet plays a key role in modulating the intestinal microbiota, thereby impacting the regulation of intestinal inflammation. The Western diet has been linked to non-communicable diseases such as obesity, cardiovascular disease, diabetes, and metabolic syndrome, and its role in inflammatory disorders is subject to increasing attention. It is of interest to understand, how maternal nutrition directly impacts fetal development and, together with the child’s own nutrition, influences the later susceptibility to immune mediated diseases. This ambitious project will assess how maternal exposures and dietary patterns during pregnancy combined with early life exposures at birth influence the risk of inflammatory bowel disease (IBD) in offspring.

Methods
We will use the Danish National Birth Cohort (about 100,000 women and 91,383 children were included in the cohort) in which 72,821 completed the food frequency questionnaire (FFQ) in early pregnancy and nationwide health data to 1) characterize the dietary patterns of mothers in mid-pregnancy, and 2) use this information to assess the association of maternal dietary pattern with risk of IBD in offspring. Furthermore, we will examine 3) the impact of diets rich in ultra-processed foods, and 4) mothers' fish consumption in mid-pregnancy and the association with risk of IBD in offspring.

Anticipated Impact
The applicant has assembled a unique team of collaborators, which, together with access to exclusive Danish resources, will guarantee the success of the project, with the primary objective is to understand how diet influences the development and worsening of IBD, improve quality of life, and to pave the way for improved dietary guidance for a growing global group of young people with chronic diseases.
Novel breast cancer susceptibility loci under linkage peaks identified in African ancestry consortia
Authors: Ochs-Balcom Heather M , Preus Leah , Du Zhaohui , Elston Robert C , Teerlink Craig C , Jia Guochong , Guo Xingyi , Cai Qiuyin , Long Jirong , Ping Jie , Li Bingshan , Stram Daniel O , Shu Xiao-Ou , Sanderson Maureen , Gao Guimin , Ahearn Thomas , Lunetta Kathryn L , Zirpoli Gary , Troester Melissa A , Ruiz-Narváez Edward A , Haddad Stephen A , Figueroa Jonine , John Esther M , Bernstein Leslie , Hu Jennifer J , Ziegler Regina G , Nyante Sarah , Bandera Elisa V , Ingles Sue A , Mancuso Nicholas , Press Michael F , Deming Sandra L , Rodriguez-Gil Jorge L , Yao Song , Ogundiran Temidayo O , Ojengbede Oladosu , Bolla Manjeet K , Dennis Joe , Dunning Alison M , Easton Douglas F , Michailidou Kyriaki , Pharoah Paul D P , Sandler Dale P , Taylor Jack A , Wang Qin , O’Brien Katie M , Weinberg Clarice R , Kitahara Cari M , Blot William , Nathanson Katherine L , Hennis Anselm , Nemesure Barbara , Ambs Stefan , Sucheston-Campbell Lara E , Bensen Jeannette T , Chanock Stephen J , Olshan Andrew F , Ambrosone Christine B , Olopade Olufunmilayo I , the Ghana Breast Health Study Team  , Conti David V , Palmer Julie , García-Closas Montserrat , Huo Dezheng , Zheng Wei , Haiman Christopher 
Published: January 2024

Abstract

Background
Expansion of genome-wide association studies across population groups is needed to improve our understanding of shared and unique genetic contributions to breast cancer. We performed association and replication studies guided by a priori linkage findings from African ancestry (AA) relative pairs.

Methods
We performed fixed-effect inverse-variance weighted meta-analysis under three significant AA breast cancer linkage peaks (3q26-27, 12q22-23, and 16q21-22) in 9241 AA cases and 10 193 AA controls. We examined associations with overall breast cancer as well as estrogen receptor (ER)-positive and negative subtypes (193,132 SNPs). We replicated associations in the African-ancestry Breast Cancer Genetic Consortium (AABCG).

Results
In AA women, we identified two associations on chr12q for overall breast cancer (rs1420647, OR = 1.15, p = 2.50×10−6; rs12322371, OR = 1.14, p = 3.15×10−6), and one for ER-negative breast cancer (rs77006600, OR = 1.67, p = 3.51×10−6). On chr3, we identified two associations with ER-negative disease (rs184090918, OR = 3.70, p = 1.23×10−5; rs76959804, OR = 3.57, p = 1.77×10−5) and on chr16q we identified an association with ER-negative disease (rs34147411, OR = 1.62, p = 8.82×10−6). In the replication study, the chr3 associations were significant and effect sizes were larger (rs184090918, OR: 6.66, 95% CI: 1.43, 31.01; rs76959804, OR: 5.24, 95% CI: 1.70, 16.16).

Conclusion
The two chr3 SNPs are upstream to open chromatin ENSR00000710716, a regulatory feature that is actively regulated in mammary tissues, providing evidence that variants in this chr3 region may have a regulatory role in our target organ. Our study provides support for breast cancer variant discovery using prioritization based on linkage evidence.
Restoration of injured motoneurons reduces microglial proliferation in the adult rat facial nucleus
Authors: Ishijima Takashi , Nakajima Kazuyuki 
Published: January 2024

Abstract
In the axotomized facial nucleus (axotFN), the levels of choline acetyltransferase, vesicular acetylcholine transporter, and gamma amino butyric acid A receptor α1 are decreased, after which the microglia begin to proliferate around injured motoneuron cell bodies. We conjectured that an injury signal released from the injured motoneurons triggers the microglial proliferation in the axotFN. However, it is unclear whether the level of microglial proliferation is dependent on the degree of motoneuronal insult. In this study, we investigated the relationship between the extents of motoneuronal injury and microglial proliferation in a rat axotFN model. Administration of glial cell line-derived neurotrophic factor, N-acetyl L-cysteine, or salubrinal at the transection site ameliorated the increase in c-Jun and the reductions in levels of phosphorylated cAMP response element binding protein (p-CREB) and functional molecules in the injured motoneurons. Concurrently, the levels of the microglial marker ionized calcium-binding adapter molecule 1 and of macrophage colony-stimulating factor (cFms), proliferating cell nuclear antigen, and p-p38/p38 were significantly downregulated in microglia. These results demonstrate that the recovery of motoneuron function resulted in the reduction in microglial proliferation. We conclude that the degree of neuronal injury regulates the levels of microglial proliferation in the axotFN.
Cone-beam computed tomography-guided online adaptive radiotherapy for pharyngeal cancer with whole neck irradiation: dose-volume histogram analysis between adapted and scheduled plans
Authors: Uehara Takuya , Nishimura Yasumasa , Ishikawa Kazuki , Inada Masahiro , Matsumoto Kenji , Doi Hiroshi , Monzen Hajime , Matsuo Yukinori 
Published: January 2024

Abstract
The present study aimed to evaluate whether an adapted plan with Ethos™ could be used for pharyngeal cancer. Ten patients with pharyngeal cancer who underwent chemoradiotherapy with available daily cone-beam computed tomography (CBCT) data were included. Simulated treatments were generated on the Ethos™ treatment emulator using CBCTs every four to five fractions for two plans: adapted and scheduled. The simulated treatments were divided into three groups: early (first–second week), middle (third–fourth week), and late (fifth–seventh week) periods. Dose-volume histogram parameters were compared for each period between the adapted and scheduled plans in terms of the planning target volume (PTV) (D98%, D95%, D50% and D2%), spinal cord (Dmax and D1cc), brainstem (Dmax) and ipsilateral and contralateral parotid glands (Dmedian and Dmean). The PTV D98%, D95% and D2% of the adapted plan were significantly higher than those of the scheduled plans in all periods, except for D98% in the late period. The adapted plan significantly reduced the spinal cord Dmax and D1cc compared with the scheduled plan in all periods. Ipsilateral and contralateral parotid glands Dmean of the adapted plan were lower than those of scheduled plan in the late period. In conclusion, the present study revealed that the adapted plans could maintain PTV coverage while reducing the doses to organs at risk in each period compared with scheduled plans.
Differential expression and evolutionary diversification of RNA helicases in Boechera sexual and apomictic reproduction
Authors: Binmöller Laura , Volkert Christopher , Kiefer Christiane , Zühl Luise , Slawinska Magdalena W , Loreth Anna , Nauerth Berit H , Ibberson David , Martinez Rafael , Mandakova Terezie M , Zipper Reinhard , Schmidt Anja 
Published: January 2024

Abstract
In higher plants, sexual reproduction is characterized by meiosis of the first cells of the germlines, and double fertilization of the egg and central cell after gametogenesis. In contrast, in apomicts of the genus Boechera, meiosis is omitted or altered and only the central cell requires fertilization, while the embryo forms parthenogenetically from the egg cell. To deepen the understanding of the transcriptional basis underlying these differences, we applied RNA-seq to compare expression in reproductive tissues of different Boechera accessions. This confirmed previous evidence of an enrichment of RNA helicases in plant germlines. Furthermore, few RNA helicases were differentially expressed in female reproductive ovule tissues harboring mature gametophytes from apomictic and sexual accessions. For some of these genes, we further found evidence for a complex recent evolutionary history. This included a homolog of Arabidopsis thaliana FASCIATED STEM4 (FAS4). In contrast to AtFAS4, which is a single-copy gene, FAS4 is represented by three homologs in Boechera, suggesting a potential for subfunctionalization to modulate reproductive development. To gain first insights into functional roles of FAS4, we studied Arabidopsis lines carrying mutant alleles. This identified the crucial importance of AtFAS4 for reproduction, as we observed developmental defects and arrest during male and female gametogenesis.
Cross-Species Comparative DNA Methylation Reveals Novel Insights into Complex Trait Genetics among Cattle, Sheep, and Goats
Authors: Chen Siqian , Liu Shuli , Shi Shaolei , Yin Hongwei , Tang Yongjie , Zhang Jinning , Li Wenlong , Liu Gang , Qu Kaixing , Ding Xiangdong , Wang Yachun , Liu Jianfeng , Zhang Shengli , Fang Lingzhao , Yu Ying 
Published: January 2024

Abstract
The cross-species characterization of evolutionary changes in the functional genome can facilitate the translation of genetic findings across species and the interpretation of the evolutionary basis underlying complex phenotypes. Yet, this has not been fully explored between cattle, sheep, goats, and other mammals. Here, we systematically characterized the evolutionary dynamics of DNA methylation and gene expression in 3 somatic tissues (i.e. brain, liver, and skeletal muscle) and sperm across 7 mammalian species, including 3 ruminant livestock species (cattle, sheep, and goats), humans, pigs, mice, and dogs, by generating and integrating 160 DNA methylation and transcriptomic data sets. We demonstrate dynamic changes of DNA hypomethylated regions and hypermethylated regions in tissue-type manner across cattle, sheep, and goats. Specifically, based on the phylo-epigenetic model of DNA methylome, we identified a total of 25,074 hypomethylated region extension events specific to cattle, which participated in rewiring tissue-specific regulatory network. Furthermore, by integrating genome-wide association studies of 50 cattle traits, we provided novel insights into the genetic and evolutionary basis of complex phenotypes in cattle. Overall, our study provides a valuable resource for exploring the evolutionary dynamics of the functional genome and highlights the importance of cross-species characterization of multiomics data sets for the evolutionary interpretation of complex phenotypes in cattle livestock.
Incidence, risk factors and outcome of postoperative acute kidney injury in China
Authors: Cheng Yichun , Nie Sheng , Zhao Xingyang , Xu Xin , Xu Hong , Liu Bicheng , Weng Jianping , Chunbo Chen , Liu Huafeng , Yang Qiongqiong , Li Hua , Kong Yaozhong , Li Guisen , Wan Qijun , Zha Yan , Hu Ying , Shi Yongjun , Zhou Yilun , Su Guobin , Tang Ying , Gong Mengchun , Hou Fan Fan , Ge Shuwang , Xu Gang 
Published: January 2024

ABSTRACT

Background
Postoperative acute kidney injury (AKI) is a common condition after surgery, however, the available data about nationwide epidemiology of postoperative AKI in China from large and high-quality studies are limited. This study aimed to determine the incidence, risk factors and outcomes of postoperative AKI among patients undergoing surgery in China.

Methods
This was a large, multicentre, retrospective study performed in 16 tertiary medical centres in China. Adult patients (≥18 years of age) who underwent surgical procedures from 1 January 2013 to 31 December 2019 were included. Postoperative AKI was defined by the Kidney Disease: Improving Global Outcomes creatinine criteria. The associations of AKI and in-hospital outcomes were investigated using logistic regression models adjusted for potential confounders.

Results
Among 520 707 patients included in our study, 25 830 (5.0%) patients developed postoperative AKI. The incidence of postoperative AKI varied by surgery type, which was highest in cardiac (34.6%), urologic (8.7%) and general (4.2%) surgeries. A total of 89.2% of postoperative AKI cases were detected in the first 2 postoperative days. However, only 584 (2.3%) patients with postoperative AKI were diagnosed with AKI on discharge. Risk factors for postoperative AKI included older age, male sex, lower baseline kidney function, pre-surgery hospital stay ≤3 days or &gt;7 days, hypertension, diabetes mellitus and use of proton pump inhibitors or diuretics. The risk of in-hospital death increased with the stage of AKI. In addition, patients with postoperative AKI had longer lengths of hospital stay (12 versus 19 days) and were more likely to require intensive care unit care (13.1% versus 45.0%) and renal replacement therapy (0.4% versus 7.7%).

Conclusions
Postoperative AKI was common across surgery type in China, particularly for patients undergoing cardiac surgery. Implementation and evaluation of an alarm system is important for the battle against postoperative AKI.
Circulating Hsa-miR499a-5p as markers in dysmobility syndrome patients: a new index for diagnosing dysmobility syndrome based on osteoporosis and predicting fracture risk
Authors: Geng Zixiang , Wang Peige , Yang Guangyue , Li Yifei , Zhao Yongfang 
Published: January 2024

Abstract

Background
Dysmobility syndrome based on osteoporosis (ODS) is a disease characterized by low bone mass and low muscle mass. Its features are high fracture and high fall risk. Falls and fractures are the most important factors affecting the quality of life and lifespan of ODS. However, there is no serum marker for the evaluation of ODS patients.
Our previous studies have shown that the expression of circulating miRNA is stable and is a good marker for disease diagnosis. Therefore, this study aims to explore potential serum markers of ODS.

Methods
A total of 78 subjects were included in this study. The data including appendicular skeletal muscle mass index, bone mineral density, bone metabolism markers, and other relevant information were collected for analysis. Real-time quantitative polymerase chain reaction was used to detect 19 miRNAs associated with muscle mass reduction. The correlation of quantitative data was analyzed by Pearson. The receiver operating characteristic curve was used to evaluate the performance of miRNA as a biomarker.

Results
In this study, we found that the muscle mass and strength of patients with ODS are significantly reduced and are negatively correlated with the risk of fracture. The hsa-miR-499a-5p is specifically downregulated in ODS, and is positively correlated with muscle mass and strength, and negatively correlated with the risk of fracture. Compared with muscle mass and strength, hsa-miR-499a-5p has better sensitivity and specificity as a diagnostic marker.

Conclusion
hsa-miR-499a-5p is a potential serum biomarker for assessing muscle function and predicting fall or fracture risk in the ODS population.
Incorporating Trauma-informed Approaches to Care in Vascularized Composite Allotransplantation
Authors: Thys Erika , Laspro Matteo , Chaya Bachar F. , Rodriguez Eduardo D. , Kimberly Laura L. 
Published: January 2024

Summary:Since 1998, vascularized composite allotransplantation (VCA) has been a growing area of research in the field of reconstructive surgery. Although conditions treated with VCA often result from a traumatic injury, there has been limited emphasis on incorporating trauma-informed care (TIC) models in VCA. Considering the importance of psychosocial well-being in VCA candidates and recipients, applying best practices in TIC to the face, upper extremity, and penile transplantation may be critical in minimizing retraumatization throughout the perioperative process. There are six main principles of TIC: safety; trustworthiness and transparency; peer support; collaboration and mutuality; empowerment and choice; and cultural, historical, and gender issues, which may be further incorporated in care for VCA patients. Evaluating for PTSD and incorporating TIC may have substantial benefit to postoperative outcomes.