bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vertebrates As Bioindicators of Climate Change
Authors: Yanish Yevheniia 
Published: October 2023

The use of living organisms for stratigraphy or as bioindicators of the state of the environment has been known since the 19th century, but mainly for these studies the remains of invertebrate animals (in particular, molluscs) are used, and from vertebrates – mouse-like rodents. In the course of the work, certain species of vertebrates and invertebrates were selected, based on the presence of which in the material it is possible to reconstruct the climatic conditions in the historical past. Such information is very important for historians and archaeologists, since it allows them to better understand the living conditions of people in the historical past.
The aim of our work is to identify species of vertebrates that are stenotopic in at least one factor and, on the basis of the findings of these species at archaeological sites, to make climatic reconstructions.
Conclusions. To date, work on the identification of other indicator species continues, we have carried out reconstructions of the paleoclimate on the basis of osteological materials found in the excavations, and for a number of monuments we have compared the results with the data of paleobotanists and soil scientists from the same areas. The similarity of the obtained results is significant, which allows us to propose a method of reconstruction of paleoclimatic and paleoecological conditions based on the findings of the indicator species selected by us as effective. The most effective is a comprehensive study of osteological materials, taking into account the presence of the above-mentioned species in the material. In terms of time spent, the identification of zooarchaeological materials is a simpler and faster method than paleobotanical research, and the results are largely comparable. In addition, the same species can be used to model predictions of habitat changes due to climate change in the future.
Archaeological Antiquities of Church and Archaeological Museum at Kyiv Theological Academy (1872-1919)
Authors: Kravchenko Inna 
Published: October 2023

The purpose of the research paper is to study the formation history of the collection of archaeological antiquities of the Church and Archaeological Museum at Kyiv Theological Academy, the ways of their acquisition by the Museum, the content of the archaeological collections, the personalities of the collectors, and the historical fate of the holdings.
Scientific novelty. The history of the formation and content of the collections of archaeological antiquities is separated from the general issue of the activities of the Church and Archaeological Society and the Museum at Kyiv Theological Academy.
Conclusions. The Church and Archaeological Museum at Kyiv Theological Academy, founded in 1872, was the largest and most significant in the territory of modern Ukraine in terms of the number, variety, and value of the objects stored. Despite its initial focus on collecting mainly church objects, during the existence of the Museum, its collection accumulated a lot of other archaeological and historical artifacts. Accumulation of antiquities in the Museum contributed to their preservation and scientific study.
Among the archaeological antiquities of the Museum were objects of the Stone, Copper, and Bronze Ages, ancient Egyptian, antique Greek and Rome, Greek colonies of the Northern Black Sea region, Hellenistic, Scythian, Sarmatian, Gothic, Slavic, Byzantine, Kyivan Rus, Lithuanian era, period of 1569-1795, and the items belonged to the Cossacks of the 18th century, etc.
Objects came from the territory of modern Ukraine, Belarus, Georgia, Russia, Egypt, Turkey, Greece, Palestine, etc. Mainly, there were artifacts from excavations and accidental finds. Collections were given to the Museum by private individuals. Among them, the greatest contribution to the enriching of holdings belonged to Archimandrite Antonin (Kapustin), Bishop Porfyrii (Uspenskyi), M.O. Leopardov, V.Z. Zavitnevich, V.I. Hoshkevich, numerous donators from the clergy, teachers, and archeology enthusiasts. The greatest contribution to the preservation, research, scientific description, and publication of the Museum’s collections belonged to M.I. Petrov.
Disasters of the 20th century (two world wars, the revolutions of 1917, and the dominance of atheistic ideology in the USSR) negatively affected the preservation of the rich collections of the Church and Archaeological Museum at Kyiv Theological Academy. Many museum objects disappeared, were stolen, or were simply destroyed; the Museum itself also ceased to exist. However, part of the collections survived, and some items from them are still stored in some Kyiv museums.
Effectiveness of an online mental health strengthening module to build resilience and overcome stress for transitional aged medical students
Authors: Kaligis Fransiska , Ismail Raden Irawati , Wiguna Tjhin , Prasetyo Sabarinah , Gunardi Hartono , Indriatmi Wresti , Pasaribu Merci Monica , Pandia Veranita , Minayati Kusuma , Magdalena Clarissa Cita , Nurraga Garda Widhi , Pramatirta Billy , Calvin Nicholas , Sourander Andre 
Published: October 2023

IntroductionTransitional-aged youths (17-to-24-years-old) are prone to mental-health problems. Students in higher education, especially medical students, are more exposed to stressors and thus need training to increase resilience. However, there have been limited mental-health strengthening modules specifically developed for medical students of transitional age, and none in Indonesia. This study intends to test the effectiveness of an online mental-health strengthening module in altering resilience.MethodsA pragmatic randomized trial with repeated measurements was employed to evaluate biopsychosocial outcomes of resilience. The intervention module was delivered in 4 weeks to 105 eligible students. Participants were divided into intervention group (n = 52) and control group (n = 53). Outcomes were measured in the 4th, 8th, and 12th weeks. Primary outcome was resilience level as measured by Connor-Davidson Resilience Scale (CD-RISC). Perceived Stress Scale (PSS), Depression Anxiety Stress Scale (DASS) were utilized to measure stress, depression and anxiety. Knowledge and attitude toward mental-health were also measured through validated questionnaires. Stress levels of participants were measured biologically by measuring salivary cortisol and alpha-amylase levels at the baseline and 12th-week.ResultsCompared to the control group, there were no significant difference in resilience score of the intervention group compared to control group [F(1, 103) = 2.243, P = .137]; however, there was a significant main effect of time [F(3, 309) = 18.191, P &lt; .001] and interaction effect between intervention and time in resilience score [F(3, 309) = 5.056, P = .002]. Additionally, compared to the control group, there were significant increases in knowledge [F(1, 103) = 66.805, P &lt; .001], attitudes and behavior towards mental-health [F(1, 103) = 5.191, P = .025], and a significant decrease in stress perception score [F(1, 103) = 27.567, P &lt; .001]. The mean salivary delta cortisol during pre-test and post-test at week 12 in the intervention group showed significant difference (P &lt; .001). However, there was no significant difference in the mean delta salivary alpha-amylase between pre-test and post-test at week 12, both in the intervention and control groups.ConclusionThe mental-health strengthening module was accepted and applicable to first-year medical students and was found to be effective in increasing resilience from various biopsychosocial aspects. It is also advisable to have similar modules throughout the medical school to maintain sustainability.
In situ U–Pb age determination of apatite from carbonatite and kimberlite in the Batain Basin, eastern Oman
Authors: Sun Jing , Jiang Leiyin , Sun Jiaxuan 
Published: October 2023

Northeastern Oman is characterized by carbonatite and kimberlite complexes, which are the ideal samples for studying the relationship between carbonatite and kimberlite. However, the ages of the Oman kimberlite and carbonatite complexes are still unknown, which restricts the understanding of the relationship between carbonatite and kimberlite in Oman. In this study, we use in situ laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) to analyze the apatite from Oman carbonatite, kimberlite, and spessartite. The U–Pb apatite ages are 141.6 ± 6.0 Ma, 137.4 ± 5.2 Ma, and 141.2 ± 6.2 Ma for carbonatite, spessartite (a kind of calc-alkaline lamprophyre), and kimberlite, respectively. These results suggest that the carbonatite and kimberlite were emplaced contemporaneously, followed by calc-alkaline carbonatite (spessartite) emplaced in the Early Cretaceous. The occurrence of carbonatite, kimberlite, and spessartite magmatism of Oman was contemporaneous with the time of the Gondwana breakup during the opening of the Indian Ocean. It is seen that 140 Ma–130 Ma is one of the strongest global kimberlite abundance peaks of the 250 Ma–50 Ma kimberlite bloom, which corresponds with the period of the Pangea supercontinent breakup. The Oman kimberlites and carbonatites are related to a distal effect of the breakup of the Gondwana portion of the Pangea supercontinent, which provided a cool, volatile-fluxed decompression-related circumstance for the formation.
Identifying potential biomarkers for non-obstructive azoospermia using WGCNA and machine learning algorithms
Authors: Tang Qizhen , Su Quanxin , Wei Letian , Wang Kenan , Jiang Tao 
Published: October 2023

ObjectiveThe cause and mechanism of non-obstructive azoospermia (NOA) is complicated; therefore, an effective therapy strategy is yet to be developed. This study aimed to analyse the pathogenesis of NOA at the molecular biological level and to identify the core regulatory genes, which could be utilised as potential biomarkers.MethodsThree NOA microarray datasets (GSE45885, GSE108886, and GSE145467) were collected from the GEO database and merged into training sets; a further dataset (GSE45887) was then defined as the validation set. Differential gene analysis, consensus cluster analysis, and WGCNA were used to identify preliminary signature genes; then, enrichment analysis was applied to these previously screened signature genes. Next, 4 machine learning algorithms (RF, SVM, GLM, and XGB) were used to detect potential biomarkers that are most closely associated with NOA. Finally, a diagnostic model was constructed from these potential biomarkers and visualised as a nomogram. The differential expression and predictive reliability of the biomarkers were confirmed using the validation set. Furthermore, the competing endogenous RNA network was constructed to identify the regulatory mechanisms of potential biomarkers; further, the CIBERSORT algorithm was used to calculate immune infiltration status among the samples.ResultsA total of 215 differentially expressed genes (DEGs) were identified between NOA and control groups (27 upregulated and 188 downregulated genes). The WGCNA results identified 1123 genes in the MEblue module as target genes that are highly correlated with NOA positivity. The NOA samples were divided into 2 clusters using consensus clustering; further, 1027 genes in the MEblue module, which were screened by WGCNA, were considered to be target genes that are highly correlated with NOA classification. The 129 overlapping genes were then established as signature genes. The XGB algorithm that had the maximum AUC value (AUC=0.946) and the minimum residual value was used to further screen the signature genes. IL20RB, C9orf117, HILS1, PAOX, and DZIP1 were identified as potential NOA biomarkers. This 5 biomarker model had the highest AUC value, of up to 0.982, compared to other single biomarker models; additionally, the results of this biomarker model were verified in the validation set.ConclusionsAs IL20RB, C9orf117, HILS1, PAOX, and DZIP1 have been determined to possess the strongest association with NOA, these five genes could be used as potential therapeutic targets for NOA patients. Furthermore, the model constructed using these five genes, which possessed the highest diagnostic accuracy, may be an effective biomarker model that warrants further experimental validation.
Exposure to outdoor artificial light at night and breast cancer risk: a population-based case-control study in two French departments (the CECILE study)
Authors: Prajapati Nirmala , Cordina-Duverger Emilie , Boileau Adélie , Faure Elodie , Guénel Pascal 
Published: October 2023

BackgroundExposure to outdoor artificial light at night (LAN) disrupts circadian rhythms and is suspected of increasing the risk of breast cancer. To date, this is an understudied aspect of environmental pollution. In this study, we sought to assess the specific role of exposure to outdoor artificial light at night in breast cancer, independently of air pollution-related effects.MethodsData from a French population-based case-control study, including 1,185 incident breast cancer cases and 1,282 controls enrolled in 2005–2007, were used. Outdoor LAN exposure data were obtained using radiance-calibrated images from the Defense Meteorological Satellite Program (DMSP) for 1995–2006 by cross-referencing the DMSP images and the geocoded locations of residences in ArcGIS. The odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were obtained using logistic regression adjusting for multiple potential confounders, including air pollution.ResultsThe OR for overall breast cancer unadjusted for air pollution per interquartile range increase in LAN exposure was 1.05 (95% CI: 0.92–1.20). The OR decreased to 0.98 (95% CI: 0.81–1.17) after adjustment for ambient NO2 levels. Subgroup analyses showed slightly higher ORs in postmenopausal women (OR per IQR increase: 1.07; 95% CI: 0.85–1.35) and a positive association for HER2-positive breast tumors (OR: 1.55; 95% CI: 1.03–2.31).ConclusionOur results do not provide evidence that outdoor LAN exposure is associated with increased risk of breast cancer. However, an association was suggested for the HER2-positive subtype of breast cancer. Further large-scale studies with more precise exposure assessment methods, including blue light and indoor exposure measurements, and considering environmental exposures correlated with LAN exposure such as air pollution, are needed.
Consensus cluster analysis of apoptosis-related genes in patients with osteoarthritis and their correlation with immune cell infiltration
Authors: Yu Enming , Zhang Mingshu , Xu Gongping , Liu Xiaoqi , Yan Jinglong 
Published: October 2023

BackgroundOsteoarthritis (OA) progression involves multiple factors, including cartilage erosion as the basic pathological mechanism of degeneration, and is closely related to chondrocyte apoptosis. To analyze the correlation between apoptosis and OA development, we selected apoptosis genes from the differentially expressed genes (DEGs) between OA and normal samples from the Gene Expression Omnibus (GEO) database, used lasso regression analysis to identify characteristic genes, and performed consensus cluster analysis to further explore the pathogenesis of this disease.MethodsThe Gene expression profile datasets of OA samples, GSE12021 and GSE55235, were downloaded from GEO. The datasets were combined and analyzed for DEGs. Apoptosis-related genes (ARGs) were collected from the GeneCards database and intersected with DEGs for apoptosis-related DEGs (ARDEGs). Least absolute shrinkage and selection operator (LASSO) regression analysis was performed to obtain characteristic genes, and a nomogram was constructed based on these genes. A consensus cluster analysis was performed to divide the patients into clusters. The immune characteristics, functional enrichment, and immune infiltration statuses of the clusters were compared. In addition, a protein–protein interaction network of mRNA drugs, mRNA-transcription factors (TFs), and mRNA-miRNAs was constructed.ResultsA total of 95 DEGs were identified, of which 47 were upregulated and 48 were downregulated, and 31 hub genes were selected as ARDEGs. LASSO regression analysis revealed nine characteristic genes: growth differentiation factor 15 (GDF15),NAMPT,TLR7,CXCL2,KLF2,REV3L,KLF9,THBD, andMTHFD2. Clusters A and B were identified, andneutrophil activationandneutrophil activation involved in the immune responsewere highly enriched in Cluster B, whereasprotein repairand purine salvagesignal pathways were enriched in Cluster A. The number of activated natural killer cells in Cluster B was significantly higher than that in Cluster A.GDF15andKLF9interacted with 193 and 32 TFs, respectively, andCXCL2andREV3Linteracted with 48 and 82 miRNAs, respectively.ConclusionARGs could predict the occurrence of OA and may be related to different degrees of OA progression.
Eotaxin-1/CCL11 promotes cellular senescence in human-derived fibroblasts through pro-oxidant and pro-inflammatory pathways
Authors: Lavandoski Patrícia , Pierdoná Vinícius , Maurmann Rafael Moura , Grun Lucas Kich , Guma Fatima T. C. R. , Barbé-Tuana Florencia María 
Published: October 2023

IntroductionEotaxin-1/CCL11 is a pivotal chemokine crucial for eosinophil homing to the lungs of asthmatic patients. Recent studies also suggest that CCL11 is involved in the aging process, as it is upregulated in elderly, and correlated with shorter telomere length in leukocytes from asthmatic children. Despite its potential pro-aging effects, the precise contribution of CCL11 and the underlying mechanisms involved in the promotion of cellular senescence remains unclear. Therefore, the primary goal of this study was to explore the role of CCL11 on senescence development and the signaling pathways activated by this chemokine in lung fibroblasts.MethodsTo investigate the targets potentially modulated by CCL11, we performed an in silico analysis using PseudoCell. We validated in vitro the activation of these targets in the human lung fibroblast cell line MRC-5 following rhCCL11 exposure. Finally, we performed differential gene expression analysis in human airway epithelial cells of asthmatic patients to assess CCL11 signaling and activation of additional senescent markers.ResultsOur study revealed that eotaxin-1/CCL11 promote reactive oxygen secretion (ROS) production in lung fibroblasts, accompanied by increased activation of the DNA damage response (DDR) and p-TP53 and γH2AX. These modifications were accompanied by cellular senescence promotion and increased secretion of senescence-associated secretory phenotype inflammatory cytokines IL-6 and IL-8. Furthermore, our data show that airway epithelial lung cells from atopic asthmatic patients overexpress CCL11 along with aging markers such as CDKN2A (p16INK4a) and SERPINE1.DiscussionThese findings provide new insights into the mechanisms underlying the pro-aging effects of CCL11 in the lungs of asthmatic patients. Understanding the role of CCL11 on senescence development may have important implications for the treatment of age-related lung diseases, such as asthma.
Comparison of cataract patients with regular corneal astigmatism after implantation of extended range-of-vision and bifocal toric intraocular lenses
Authors: Li Zhuoya , Guo Rong , Hu Xiaomin , Yang Xinyue , Wen Ziyuan , Lin Yi , Zhang Hui 
Published: October 2023

PurposeTo compare the postoperative visual acuity and visual quality between extended range-of-vision and multifocal toric intraocular lens (IOLs) after implantation in cataract patients with regular corneal astigmatism.SettingDepartment of Ophthalmology, the Second Hospital of Jilin University, Changchun, Jilin Province, China.DesignRetrospective and single-center study.MethodsThe study involved implanting the Tecnis Symphony (ZXR00IOL) or the bifocal toric (ZMTIOL) in patients undergoing cataract surgery. Three months after surgery, lens performance was evaluated using distance, intermediate, and near visual acuity tests, defocus curves, the modulation transfer function (MTF), a visual function index questionnaire (VF-14), and the adverse optical interference phenomena.ResultsThe 3-month postoperative follow-up found that both groups had good corrected distance vision. The ZMT group had better-uncorrected distance visual acuity and near visual acuity (p &lt; 0.05). However, the ZXR group showed better uncorrected intermediate visual acuity (p &lt; 0.05) and visual continuity. Overall astigmatism in the postoperative ZMT group was significantly lower than that in the pre-operative group (p &lt; 0.05). The ZMT group had lower total high-order aberrations (tHOs), higher MTF values, and higher VF-14 scores (p &lt; 0.05). Finally, the ZXR group exhibited reduced halo and glare phenomena (p &lt; 0.05).ConclusionWe found that ZMT can effectively correct a corneal astigmatism of 1.0–1.5 D and ZXR can improve patient outcomes regarding subjective optical quality and range of vision. These findings have the potential to improve future astigmatism treatment options.
Analysis of distribution and antibiotic resistance of Gram-positive bacteria isolated from a tertiary-care hospital in southern China: an 8-year retrospective study
Authors: Zhang Xiao , Tan Liming , Ouyang Pengwen , Ma Haiyan , Peng Jianqiao , Shi Ting , Xie Liangyi 
Published: October 2023

ObjectiveDue to the severe drug resistance situation of Gram-negative bacteria, especially Gram-negative enterobacter, relatively little attention has been paid to the changes in Gram-positive bacteria species and drug resistance. Therefore, this study analyzed the prevalence and drug resistance of Gram-positive bacteria in a general tertiary-care hospital from 2014 to 2021, in order to discover the changes in Gram-positive bacteria distribution and drug resistance that cannot be easily identified, inform clinicians in their respective regions when selecting antimicrobial agents, and to provide the basis for the diagnosis of Gram-positive bacterial infection, and for the comprehensive and multi-pronged prevention and control of drug-resistant bacteria.MethodsA retrospective study was conducted on Gram-positive bacteria isolated from patients presented to a general tertiary-care hospital from January 2014 to December 2021. A total of 15,217 Gram-positive strains were analyzed.ResultsDuring the 8-year period, the total number and the species of Gram-positive bacteria isolated from clinic increased continuously. The seven most common species were Streptococcus pneumoniae (21.2%), Staphylococcus aureus (15.9%), Enterococcus faecium (20.6%), Enterococcus faecalis (14.0%), and Staphylococcus epidermidis (7.8%), Staphylococcus haemolyticus (4.8%), Streptococcus agalactiae (3.6%). The isolation rates of Staphylococcus aureus and Streptococcus agalactiae increased, and the isolation rate of Enterococcus faecium decreased. The resistance rates of Staphylococcus aureus to erythromycin, clindamycin, tetracycline, rifampicin and furantoin decreased obviously. The resistance rates of Streptococcus pneumoniae to cefepime (non-meningitis) and ceftriaxone (meningitis) decreased significantly. The resistance rates of Enterococcus faecium to penicillin, ampicillin, erythromycin, levofloxacin, ciprofloxacin and furantoin rose rapidly from 50.3, 47.6, 71.5, 44.9, 52.3, and 37.5% in 2014 to 93.1, 91.6, 84.9, 86.8, 86.8, and 60.0% in 2021, respectively.ConclusionThe total number and the species of Gram-positive bacteria isolated during the 8-year period increased continuously. Streptococcus pneumoniae and Staphylococcus aureus are the main causes of positive bacterial infections in this hospital. The resistance rates of Enterococcus faecium to a variety of commonly used antibiotics increased significantly. Therefore, it is very important to monitor the distribution of bacteria and their resistance to antibiotics to timely evaluate and identify changes in drug resistance that are not easily detected.