bluuebunny/crossref_metadata_2025_split huggingface dataset:
DPSS yellow‐green 561‐nm lasers for improved fluorochrome detection by flow cytometry
Authors: Telford William , Murga Matilde , Hawley Teresa , Hawley Robert , Packard Beverly , Komoriya Akira , Haas Fred , Hubert Charles 
Published: September 2005

AbstractIntroductionBlue‐green 488‐nm laser sources are widespread in flow cytometry but suffer some drawbacks for cell analysis, including their excitation of endogenous proteins (resulting in high cellular autofluorescence) and their less‐than‐optimal coincidence with the excitation maxima of commonly used fluorochromes, including the phycoerythrins (PE). Longer wavelength lasers such as green helium–neons and, more recently, diode‐pumped solid state (DPSS) 532‐nm sources have previously been employed to overcome these difficulties and improve overall sensitivity for PE. In this study, we evaluate an even longer wavelength DPSS 561‐nm for its ability to improve PE and DsRed fluorescent protein detection sensitivity.MethodsA DPSS 561‐nm laser emitting at 10 mW was mounted onto a BD LSR II. Mouse thymoma cells labeled with cell surface marker antibodies conjugated to the R‐ and B‐forms of PE were analyzed and compared with conventional 488‐nm excitation using the same bandpass filters and signal travel distances. A similar analysis was carried out with cell lines expressing the red fluorescent protein DsRed, several green‐yellow excited low molecular weight fluorochromes, and a rhodamine‐based caspase substrate. Additionally, cells labeled with PE and co‐labeled with fluorescein or simultaneously expressing green fluorescent protein (GFP) were analyzed to determine if PE excitation at 561 nm with simultaneous fluorescein/GFP detection was feasible.ResultsThe DPSS 561‐nm laser gave a several‐fold improvement in the fluorochrome to autofluorescence ratios between PE‐labeled cells and unlabeled controls. Analysis of cells expressing the fluorescent protein DsRed with the DPSS 561‐nm source gave a 6–7‐fold improvement in sensitivity over 488‐nm excitation, and gave excellent excitation of yellow‐green excited fluorochromes and rhodamine‐based physiological probes. Yellow‐green laser light also caused virtually no impingement on the spatially separated fluorescein/GFP detector, a significant problem with green laser sources, and also allowed simultaneous analysis of GFP and PE with virtually no signal overlap or requirement for color compensation.ConclusionsDPSS 561‐nm laser excitation gave significantly improved sensitivity for both PE‐labeled and DsRed expressing cells, with little contamination of a typical fluorescein/GFP detector. Published 2005 Wiley‐Liss, Inc.
Melanoma cell necrosis facilitates transfer of specific sets of antigens onto MHC class II molecules of dendritic cells
Authors: Röhn Till A. , Schadendorf Dirk , Sun Yuansheng , Nguyen Xuan Duc , Roeder Daniel , Langen Hanno , Vogt Anne B. , Kropshofer Harald 
Published: September 2005

AbstractVaccine strategies that target dendritic cells (DC) in order to elicit immunity against tumors are the subject of intense research. For the induction and maintenance of anti‐tumor immunity, CD4+ helper T cells are often required, which need to see appropriate MHC class II‐peptide complexes on DC. So far, it remained widely unclear what type of tumor cells can feed the MHC class II processing pathway of DC with what type of antigens. Here, we report that peptide loading onto MHC class II molecules of myeloid DC is facilitated by melanoma cells undergoing necrotic rather than apoptotic cell death. Importantly, the set of MHC class II‐associated peptides induced by necrotic tumor cells differed from those found upon engagement of apoptotic tumor cells. This may be due to the fact that only necrotic cells liberated heat shock proteins, which bind tumor‐derived peptides and thereby may promote processing by DC. The potential of DC to activate T cells was kinetically controlled through their antigen receptivity: CD4+ T cells were easily stimulated upon encountering antigen early in DC maturation, whereas antigen capture at later maturation stages favored activation of CD8+ T cells. These findings may aid in designing future vaccination strategies and in identifying novel tumor‐specific helper T cell antigens.
Assessment of CD8 involvement in T cell clone avidity by direct measurement of HLA‐A2/Mage3 complex density using a high‐affinity TCR like monoclonal antibody
Authors: Bernardeau Karine , Gouard Sébastien , David Gaëlle , Ruellan Anne‐Lise , Devys Anne , Barbet Jacques , Bonneville Marc , Chérel Michel , Davodeau François 
Published: September 2005

AbstractPeptide affinity for MHC molecules determines the number of MHC/peptide complexes stabilized at the cell surface in in vitro tests or in vaccination protocols. We isolated a high affinity monoclonal antibody specific for the HLA‐A2/Mage3 complex that enables an equilibrium binding assay to be performed on T2 cell line loaded with a range of Mage3 peptides. Binding of Mage3 to the HLA‐A2 molecule can be modeled by a standard receptor‐ligand interaction characterized by an affinity constant. This model enables the measurement of the affinity of other immunogenic peptides for HLA‐A2 by a competition test and the calculation of the density of complexes stabilized at the T2 cell surface for all peptide concentrations. Quantification of the HLA‐A2/Mage3 complexes at target cell surfaces was used to estimate the number of complexes required to reach cytotoxicity ED50 of human T cell clones sorted from an unprimed repertoire. We confirm with this antibody the direct relationship between clone avidity and TCR affinity, and the moderate contribution of the CD8 co‐receptor in the reinforcement of TCR‐MHC/peptide contact. Nevertheless, CD8 plays a critical role in the amplification of the specific signal to establish an efficient T cell response at low specific complex densities found in physiological situations.
Clinical applications of neuroimaging with susceptibility‐weighted imaging
Authors: Sehgal Vivek , Delproposto Zachary , Haacke E. Mark , Tong Karen A. , Wycliffe Nathaniel , Kido Daniel K. , Xu Yingbiao , Neelavalli Jaladhar , Haddar Djamel , Reichenbach Jürgen R. 
Published: September 2005

AbstractSusceptibility‐weighted imaging (SWI) consists of using both magnitude and phase images from a high‐resolution, three‐dimensional, fully velocity compensated gradient‐echo sequence. Postprocessing is applied to the magnitude image by means of a phase mask to increase the conspicuity of the veins and other sources of susceptibility effects. This article gives a background of the SWI technique and describes its role in clinical neuroimaging. SWI is currently being tested in a number of centers worldwide as an emerging technique to improve the diagnosis of neurological trauma, brain neoplasms, and neurovascular diseases because of its ability to reveal vascular abnormalities and microbleeds. J. Magn. Reson. Imaging 2005. © 2005 Wiley‐Liss, Inc.
Managing enterprise resource planning systems
Authors: Dery Kristine , Grant David , Hall Richard , Wailes Nick 
Published: September 2005

Abstract

The last decade has seen a dramatic growth in the number of organizations adopting Enterprise Resource Planning systems (ERPs).

To date, much of the research on ERPs has focused on the process of implementation, with relatively little attention given to their ongoing impact on organizations or how to effectively manage these systems over time. The papers in this special issue explore these issues.

ERPs require a considerable financial investment and produce widespread change in organizations. The papers in this special issue suggest that for organizations to gain strategic benefit from ERPs, managers have to be prepared to make a long‐term investment of organizational resources and to develop the capabilities necessary to manage them.

Copyright © 2005 John Wiley & Sons, Ltd.
Determination of 12 pueraria components by high‐performance liquid chromatography‐mass spectrometry
Authors: Lin Ching‐Che , Wu Chuan‐I. , Sheu Shuenn‐Jyi 
Published: September 2005

AbstractPuerariae radix, a commonly used Chinese herb drug derived from the dried root of legume plant, contains a series of isoflavones as its chief pharmacologically active constituents. Using 12 pueraria components as markers, an LC‐UV‐MS method requiring less than 60 min, was developed for estimating the quality of pueraria samples within 60 min. Extracts were analyzed using a Cosmosil 5C18‐MS column, by gradient elution with an aqueous solution of acetic acid and methanol–ACN at a flow‐rate of 1.0 mL/min. Peaks were detected at 254 nm and each peak was identified by LC/MS. The reproducibilities (RSD) of this proposed method, on the basis of peak‐area ratios from six replicate injections, were 0.93–1.42% (intraday) and 1.05–1.63% (interday) at a detection limit of 0.12–0.78 μg/mL. Most of the markers used in this study can be classified, respectively, into three major categories, namely, isoflavones, O‐glycosidic isoflavones, and C‐glycosidic isoflavones. The structures of the compounds were determined from LC‐MS fragmentation data and data from the literature.
Comparing monolithic and microparticular capillary columns for the separation and analysis of peptide mixtures by liquid chromatography‐mass spectrometry
Authors: Toll Hansjörg , Wintringer Reiner , Schweiger‐Hufnagel Ulrike , Huber Christian G. 
Published: September 2005

AbstractA mixture of ten proteins was trypsinized and injected onto poly‐(styrene‐divinylbenzene) monolithic columns (60×0.20 or 0.10 mm ID) and a column packed with C18 silica particles (75×0.075 mm ID), respectively. The columns were eluted at 200–2000 nL/min with gradients of ACN in 0.050% TFA. Eluting peptides were detected by ESI‐MS/MS and subsequently identified by database searching. The 100 μm ID monolithic column showed the highest cumulative Mowse scores based on the highest ion scores for the peptides and the largest number of identified peptides. It is shown that the number of identified peptides strongly depends on the dynamic range within the peptide mixture. In consequence, all proteins were identified in a mixture of relatively balanced analyte amounts (12.5–80 fmol) whereas only peptides for six out of ten proteins were found in a sample of high‐dynamic range (0.65–270 fmol). The 100 μm monolithic column showed the highest reproducibility for peptide identifications in three consecutive runs. Depending on sample amount, 57–72% of the identified peptides were detectable in each of the three runs of triplicate analyses. The results demonstrate the high suitability of 100 μm monolithic columns for high‐resolution peptide separations in proteomic research.
Simultaneous determination of carotenoids, tocopherols, and γ‐oryzanol in crude rice bran oil by liquid chromatography coupled to diode array and mass spectrometric detection employing silica C30 stationary phases
Authors: Stöggl Wolfgang , Huck Christain , Wongyai Surapote , Scherz Heimo , Bonn Günther 
Published: September 2005

AbstractCrude rice bran oil contains tocopherols (vitamin E), carotenoids (vitamin A), and phytosterols, which possess antioxidant activities and show promising effects as preventive and therapeutic agents. The aim of this work was to establish methods and to compare C18 and C30 silica stationary phases in order to separate and detect tocopherols, carotenoids, and γ‐oryzanol in one single run. Comparing RP‐LC on silica C18 and C30, higher resolution between all target compounds was obtained using the C30 stationary phase. Methanol was used as eluent and the elution strength was increased by the addition of tert‐butyl methyl ether for highly hydrophobic analytes such as γ‐oryzanol. Detection was accomplished by diode array detection from 200 to 500 nm. Absorbance maxima were found at 295 nm for tocopherols, 324 nm for γ‐oryzanol, and 450 nm for carotenoids. Furthermore, compounds were characterized and identified on the basis of their UV‐spectra. Both RP systems were coupled to MS (LC‐MS) by using an atmospheric pressure chemical ionization interface.
Corrosion characteristics of the wrought Ni‐Cr‐Mo alloys
Authors: Crook P. 
Published: September 2005

AbstractThis paper concerns the wrought, nickel‐chromium‐molybdenum (Ni‐Cr‐Mo) alloys, a family of materials with a long history of use in the chemical process industries. Their attributes include resistance to the halogen acids and resistance to pitting, crevice attack, and stress corrosion cracking in hot, halide salt solutions. The purpose of this paper is to characterize the performance of the Ni‐Cr‐Mo alloys in several key chemicals, using iso‐corrosion diagrams. These indicate the expected corrosion rates over wide ranges of concentration and temperature. Furthermore, the differences between individual Ni‐Cr‐Mo alloys, and their behavior relative to the stainless steels are defined. The data indicate benefits of both a high chromium content and a copper addition, as used in Hastelloy® C‐2000® alloy.
Effect of (R+, X–) salts addition on nickel corrosion in 1M sulfuric acid medium
Authors: Said F. , Souissi N. , Dermaj A. , Hajjaji N. , Triki E. , Srhiri A. 
Published: September 2005

AbstractWe used the electrochemical techniques to study the effect of the addition of four salts, K+,Cl−, K+,Br−, K+,I− and C8H17Ph3P+,I−, on the corrosion behaviour of nickel when submitted to 1M sulphuric acid medium. After one hour of immersion time, we noted an acceleration of nickel corrosion process after the addition of K+,Cl− and K+,Br− in the 1M H2SO4 electrolyte. K+,I− exhibited an inhibition of the anodic Ni corrosion process whereas the phosphonium iodine (R+,I−) acts simultaneously on both processes: it is a mixed inhibitor. Such result is due to the adsorption of the alkyl group C8H17 which limited oxygen diffusion.EIS characterization of Ni exhibited surfaces after iodine salts addition allowed us simulating the resulting interfaces by electrical equivalent circuits. We concluded that (K+,I−) addition decreased the material corrosion rate without changing the alteration mechanism. That result is due to the formation of NiI at the electrode surface. On the other hand, phosphonium iodine addition modifies the interface behaviour. In fact, the alkyl part of the organic iodine adsorbs at the surface preserving it against the corrosive action of the sulfuric acid.
The study of the La(NO;3)3 inhibition on X70 pipeline steel in 3.0(wt.%) NaCl solution
Authors: Zhang L.J. , Zhang Z. , Zhang J.Q. , Cao C.N. 
Published: September 2005

AbstractLanthanum nitrate has been investigated as corrosion inhibitor of X70 pipeline steel in 3.0 (wt.%) NaCl solution using electrochemical techniques such as potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) in conjunction with scanning electron microscopy (SEM) and energy dispersive X‐ray detector (EDX) techniques. The results showed that La(NO3)3 belonged to the film‐formed mixed inhibitors, which can form a compact network over the whole surface of X70 pipeline steel. The inhibition effect of La(NO3)3 on X70 steel reinforced with the increase of the concentration itself. The EDX analysis showed that lanthanum ions were found to participate in the network film formed on the sample surface.
Evaluation of a biocide effect upon microbiologically influenced corrosion of mild steel
Authors: Hernández Gayosso M. J. , Zavala Olivares G. , Ruiz Ordaz N. , García Esquivel R. 
Published: September 2005

AbstractThe microbiologically influenced corrosion (MIC) is a very dangerous process, which affects the oil industry. The activity and microorganisms' growth at the pipelines steel cause surface modifications, which can induce a more complex corrosion process. The biocide evaluation for the MIC decrease has been normally based upon microbiological tests, and just a few references mention alternating methods which can be used as criteria for their evaluation. In this work, a commercial biocide was tested, using different electrochemical laboratory techniques, to determine its effect upon a biofilm generating bacteria consortium.Using microbiological techniques, the biocide lethal concentration was determined, and a concentration of 200 ppm was used to kill completely the consortium population in both, plancktonic and sessile parts. The electrochemical techniques: Polarisation Resistance (PR) and Electrochemical Impedance Spectroscopy (EIS), allowed describing the corrosion process associated to the microbial consortium and the biocide effect upon it.
An ultra‐sulfur Hastelloy alloy C‐4
Authors: Xiaobo L. , Youqing X. , Yaoxiao Z. 
Published: September 2005

AbstractThe effect of sulfur on solidification‐segregation and corrosion resistant properties of Hastelloy C‐4 corrosion‐resistant alloy was studied. It was found that strict controlling the content of sulfur can reduce the width and temperature interval of the solid‐liquid biphase region and the segregation degree of the alloy, resulting in a higher corrosion resistance. An improved low segregation Hastelloy C‐4 corrosion resistant alloy was developed.
Binding and Sulfonation of Poly(acrylic acid) on Iron Oxide Nanoparticles: a Novel, Magnetic, Strong Acid Cation Nano‐Adsorbent
Authors: Mak Sou‐Yee , Chen Dong‐Hwang 
Published: September 2005

AbstractSummary: A novel, magnetic, strong acid cation nano‐adsorbent has been developed by the covalent binding of poly(acrylic acid) on the surface of Fe3O4 nanoparticles followed by sulfonation using sulfanilic acid via carbodiimide activation. The nano‐absorbent can be easily recovered or manipulated with an external magnetic field and shows a good capacity for the rapid and efficient adsorption of multivalent metal cations from aqueous solutions.An illustration for the binding and sulfonation of PAA on Fe3O4 nanoparticles to produce a magnetic, strong acid cation nano‐adsorbent.magnified imageAn illustration for the binding and sulfonation of PAA on Fe3O4 nanoparticles to produce a magnetic, strong acid cation nano‐adsorbent.
Saccharide Sensing Based on Saccharide‐Induced Conformational Changes in Fluorescent Boronic Acid Polymers
Authors: Kanekiyo Yasumasa , Sato Hiroaki , Tao Hiroaki 
Published: September 2005

AbstractSummary: A novel fluorometric saccharide detection strategy has been established by utilizing saccharide‐induced conformational changes in copolymers containing boronic acid and fluorescent units. The polymer chain changes its conformation due to an alteration of the charge state induced by binding with saccharides. The conformational changes are conveniently detected by monitoring excimer to monomer intensity ratio in the fluorescence spectra. Thus, the present strategy would be a promising way for the creation of highly selective and sensitive saccharide‐sensing systems.Saccharide‐induced conformational change in copolymers containing boronic acid units.magnified imageSaccharide‐induced conformational change in copolymers containing boronic acid units.
Microfocus X‐Ray Scattering Scanning Microscopy for Polymer Applications
Authors: Zafeiropoulos Nikolaos E. , Davies Richard J. , Roth Stephan V. , Burghammer Manfred , Schneider Konrad , Riekel Christian , Stamm Manfred 
Published: September 2005

AbstractSummary: The fracture properties of polymers are one of the key parameters that define their service life and limit their applications. One of the most interesting and important questions is how the molecular architecture and the structure of polymers at nanolength scales influence their fracture properties. X‐ray scattering is a powerful means of probing bulk structures at the nanometre scale. It can therefore provide a wealth of information relating to such structure‐property relationships. In the present study, synchrotron radiation microfocus small‐angle X‐ray scattering is used to investigate the damage area ahead and around the crack tip in polyamide 6 (PA6). The results reveal that the damage area propagates far beyond the visible crack, and inside the damaged zone platelet‐shaped cracks/voids are formed.
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A hybrid qualitative method for pretesting questionnaires: The example of a questionnaire to caregivers of Alzheimer disease patients
Authors: Oremus Mark , Cosby Jarold L. , Wolfson Christina 
Published: September 2005

AbstractA hybrid method based on cognitive interviewing and consensus panels was developed to pretest a questionnaire for caregivers of persons with Alzheimer disease (AD). The objective of the questionnaire was to elicit caregivers' attitudes and opinions on the use of medications to treat the disease. Thirty‐one caregivers were divided into five pretest groups, within which each participant was asked to comment on questionnaire wording and design. The comments from participants in the first three groups were used to revise the questionnaire, and the revised version was given to participants in the remaining two groups. Overall, 81% (118/146) of the participants' comments were implemented. The number of comments made in the last two groups decreased relative to the number of comments made in the first three groups. The hybrid method enhanced the user‐friendliness of the questionnaire and can serve as an alternative to common ad hoc pretest approaches that have little basis in theory. © 2005 Wiley Periodicals, Inc. Res Nurs Health 28: 419–430, 2005.
Resident–caregiver relationships and thriving among nursing home residents
Authors: Bergland Ådel , Kirkevold Marit 
Published: September 2005

AbstractThe purpose of this study was to describe nursing home residents' experiences and perceptions of relationships with caregivers and to explore their importance to thriving. Data were collected through field observations and open‐ended interviews with 26 residents. Receiving appropriate care from kind caregivers made life easier for all residents but contributed to thriving only for some. One group of residents emphasized personal relationships to individual caregivers as essential to thriving. A second group reported that non‐personal relationships contributed to thriving. A third group preferred distant relationships to the caregivers but reported thriving as well. Personal relationships to individual caregivers were not essential to thriving for all residents. © 2005 Wiley Periodicals, Inc. Res Nurs Health 28: 365–375, 2005.
Decisional balance for exercise: Adaptation of an instrument for older Mexican American women
Authors: Laffrey Shirley Cloutier , Lee Young Shin 
Published: September 2005

AbstractThe purpose of this study was to adapt and test an instrument to measure decisional balance for exercise with older Mexican American women. Items were derived from a Decisional Balance Scale (DBS) developed by Marcus, Rakowski, and Rossi (1992) and a focus group with Mexican American women aged 60 to 86 years. The adapted Spanish‐English DBS was administered to 75 Mexican American women (38 exercisers and 37 non‐exercisers). A factor analysis yielded a positive and a negative factor. Alpha coefficients were .95 for the positive factor and .79 for the negative factor. Decisional balance for exercise was significantly more positive for the exercisers than for the non‐exercisers and was positively related to exercise readiness and habitual activity. The findings suggest that the adapted Spanish‐English DBS can be used in exercise and activity research with older Mexican American women. © 2005 Wiley Periodicals, Inc. Res Nurs Health 28: 388–397, 2005.