bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear localization of Annexin A7 during murine brain development
Authors: Rick Michaela , Ramos Garrido Soraya I , Herr Claudia , Thal Dietmar R , Noegel Angelika A , Clemen Christoph S 
Published: April 2005

Abstract
Background
Annexin A7 is a member of the annexin protein family, which is characterized by its ability to interact with phospholipids in the presence of Ca2+-ions and which is thought to function in Ca2+-homeostasis. Results from mutant mice showed altered Ca2+-wave propagation in astrocytes. As the appearance and distribution of Annexin A7 during brain development has not been investigated so far, we focused on the distribution of Annexin A7 protein during mouse embryogenesis in the developing central nervous system and in the adult mouse brain.

Results
Annexin A7 is expressed in cells of the developing brain where a change in its subcellular localization from cytoplasm to nucleus was observed. In the adult CNS, the subcellular distribution of Annexin A7 depends on the cell type. By immunohistochemistry analysis Annexin A7 was detected in the cytosol of undifferentiated cells at embryonic days E5–E8. At E11–E15 the protein is still present in the cytosol of cells predominantly located in the ventricular germinative zone surrounding the lateral ventricle. Later on, at embryonic day E16, Annexin A7 in cells of the intermediate and marginal zone of the neopallium translocates to the nucleus. Neuronal cells of all areas in the adult brain present Annexin A7 in the nucleus, whereas glial fibrillary acidic protein (GFAP)-positive astrocytes exhibit both, a cytoplasmic and nuclear staining. The presence of nuclear Annexin A7 was confirmed by extraction of the nucleoplasm from isolated nuclei obtained from neuronal and astroglial cell lines.

Conclusion
We have demonstrated a translocation of Annexin A7 to nuclei of cells in early murine brain development and the presence of Annexin A7 in nuclei of neuronal cells in the adult animal. The role of Annexin A7 in nuclei of differentiating and mature neuronal cells remains elusive.
Development and evaluation of a hand held computer based on-call pack for health protection out of hours duty: A pilot study
Authors: Abubakar Ibrahim , Williams Christopher J , McEvoy Marian 
Published: April 2005

Abstract
Background
The on call service for health protection in most parts of the UK is provided by general public health consultants, registrars and nurses as the first tier of response backed up by medical consultants in health protection. The first tier responder usually carries a large bag of papers containing both local and national guidance on the management of common cases/incidents. An electronic on call pack may provide a suitable practical alternative to large paper based systems and help professionals deliver out of hours health protection advice and response to incidents.

Methods
We developed and piloted an electronic on call pack in Hertfordshire for use at the health protection unit level containing key local and national guidelines, contact information and useful references. The on-call pack was initially piloted using a laptop and more recently using a personal digital assistant (PDA). The use of the on-call pack was evaluated.

Results
Key advantages of the electronic system include reduced size, faster access to information that is clearly indexed and the relative ease of updating information. As part of the pilot, the electronic on call pack was presented to a local and regional training meeting with good response from participants using qualitative and quantitative methods.

Conclusion
It is anticipated that with suitable evaluation this system can be adapted and utilised by other health protection practitioners. This system provides a fast, reliable and easily maintained source of information for the public health on-call team.
The effect of executive walk rounds on nurse safety climate attitudes: A randomized trial of clinical units
Authors: Thomas Eric J , Sexton J Bryan , Neilands Torsten B , Frankel Allan , Helmreich Robert L 
Published: April 2005

Abstract
Background
Executive walk rounds (EWRs) are a widely used but unstudied activity designed to improve safety culture in hospitals. Therefore, we measured the impact of EWRs on one important part of safety culture – provider attitudes about the safety climate in the institution.

Methods
Randomized study of EWRs for 23 clinical units in a tertiary care teaching hospital. All providers except physicians participated. EWRs were conducted at each unit by one of six hospital executives once every four weeks for three visits. Providers were asked about their concerns regarding patient safety and what could be done to improve patient safety. Suggestions were tabulated and when possible, changes were made. Provider attitudes about safety climate measured by the Safety Climate Survey before and after EWRs. We report mean scores, percent positive scores (percentage of providers who responded four or higher on a five point scale (agree slightly or agree strongly), and the odds of EWR participants agreeing with individual survey items when compared to non-participants.

Results
Before EWRs the mean safety climate scores for nurses were similar in the control units and EWR units (78.97 and 76.78, P = 0.458) as were percent positive scores (64.6% positive and 61.1% positive). After EWRs the mean safety climate scores were not significantly different for all providers nor for nurses in the control units and EWR units (77.93 and 78.33, P = 0.854) and (56.5% positive and 62.7% positive). However, when analyzed by exposure to EWRs, nurses in the control group who did not participate in EWRs (n = 198) had lower safety climate scores than nurses in the intervention group who did participate in an EWR session (n = 85) (74.88 versus 81.01, P = 0.02; 52.5% positive versus 72.9% positive). Compared to nurses who did not participate, nurses in the experimental group who reported participating in EWRs also responded more favorably to a majority of items on the survey.

Conclusion
EWRs have a positive effect on the safety climate attitudes of nurses who participate in the walk rounds sessions. EWRs are a promising tool to improve safety climate and the broader construct of safety culture.
Physicochemical characterization of vibriophage N5
Authors: Sen Anindito , Ghosh Amar N 
Published: April 2005

AbstractPhage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70: 2241–2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.
Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR
Authors: Kleiboeker Steven B 
Published: April 2005

Abstract
Although PCR and RT-PCR provided a valuable approach for detection of pathogens, the high level of sensitivity of these assays also makes them prone to false positive results. In addition to cross-contamination with true positive samples, false positive results are also possible due to "carry-over" contamination of samples with amplicon DNA generated by previous reactions. To reduce this source of false positives, amplicon generated by reactions in which dUTP was substituted for dTTP can be degraded by uracil DNA glycosylase (UNG). UNG does not degrade RNA but will cleave contaminating uracil-containing DNA while leaving thymine-containing DNA intact. The availability of heat-labile UNG makes use of this approach feasible for RT-PCR. In this study, real-time RT-PCR was used to quantify UNG degradation of amplicon DNA and the effect of UNG on RNA detection. Using the manufacturers' recommended conditions, complete degradation of DNA was not observed for samples containing 250 copies of amplicon DNA. Doubling the UNG concentration resulted in degradation of the two lowest concentrations of DNA tested, but also resulted in an increase of 1.94 cycles in the CT for RNA detection. To improve DNA degradation while minimizing the effect on RNA detection, a series of time, temperature and enzyme concentrations were evaluated. Optimal conditions were found to be 0.25 U UNG per 25 μl reaction with a 20 min, 30°C incubation prior to RT-PCR. Under these conditions, high concentrations of amplicon DNA could be degraded while the CT for RNA detection was increased by 1.2 cycles.
Phase I Study of Oblimersen Sodium, an Antisense to Bcl-2, in Untreated Older Patients With Acute Myeloid Leukemia: Pharmacokinetics, Pharmacodynamics, and Clinical Activity
Authors: Marcucci Guido , Stock Wendy , Dai Guowei , Klisovic Rebecca B. , Liu Shujun , Klisovic Marko I. , Blum William , Kefauver Cheryl , Sher Dorie A. , Green Margaret , Moran Mollie , Maharry Kati , Novick Steven , Bloomfield Clara D. , Zwiebel James A. , Larson Richard A. , Grever Michael R. , Chan Kenneth K. , Byrd John C. 
Published: April 2005

Purposes Pharmacologic downregulation of Bcl-2, an antiapoptotic protein overexpressed in cancer, might increase chemosensitivity in acute myeloid leukemia (AML). Herein, we investigated the feasibility of this approach in untreated elderly AML patients by administering oblimersen sodium (G3139), an 18-mer phosphorothioate antisense to Bcl-2, during induction and consolidation treatments. Patients and Methods Untreated patients with primary or secondary AML (stratified to cohort 1 or 2, respectively) who were ≥ 60 years received induction with G3139, cytarabine, and daunorubicin at one of two different dose levels (45 and 60 mg/m2) and, on achievement of complete remission (CR), consolidation with G3139 and high-dose cytarabine. An enzyme-linked immunosorbent assay (ELISA) –based assay was used to measure plasma and intracellular concentrations (IC) of G3139. Bcl-2 mRNA and protein levels were quantified by real-time reverse transcriptase polymerase chain reaction and ELISA, respectively, in bone marrow samples collected before induction treatment and after 72 hours of G3139 infusion, prior to initiation of chemotherapy. Results Of the 29 treated patients, 14 achieved CR. With a median follow-up of 12.6 months, seven patients had relapsed. Side effects of this combination were similar to those expected with chemotherapy alone and were not dose limiting at both dose levels. After 72-hour G3139 infusion, Bcl-2/ABL mRNA copies were decreased compared with baseline (P = .03) in CR patients and increased in nonresponders (NRs; P = .05). Changes in Bcl-2 protein showed a similar trend. Although plasma pharmacokinetics did not correlate with disease response, the median IC of the antisense was higher in the CR patients compared with NRs (17.0 v 4.4 pmol/mg protein, respectively; P = .05). Conclusion G3139 can be administered safely in combination with intensive chemotherapy, and the degree of Bcl-2 downmodulation may correlate with response to therapy.
Cr3+‐Doped LiNbO3 Crystals Grown by the Bridgman Method.
Authors: Xia Haiping , Song Hongwei , Wang Jinhao , Zhang Jiahua , Wang Tie , Zhang Jianli , Zhang Yuepin , Nie Qiuhua 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Model High Surface Area Alumina‐Supported Palladium Catalyst.
Authors: Lear Timothy , Marshall Robert , Gibson Emma K. , Schuett Thomas , Klapoetke Thomas M. , Rupprechter Guenther , Freund Hans‐Joachim , Winfield John M. , Lennon David 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
The First Example of Solvothermally Synthesized Thioantimonates(V) with Ethylenediamine Coordinated Lanthanide(III): [Sm(en)4]SbS4×0.5en and [Sm(en)3(H2O)(μ‐SbS4)] ∞
Authors: Jia Ding‐xian , Zhu Qin‐Yu , Dai Jie , Lu Wen , Guo Wen‐Juan 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Open‐Framework Polar Compounds: Synthesis and Characterization of Rare‐Earth Polyoxometalates (C6NO2H5)2 [Ln(H2O)5 (CrMo6H6O24)]×0.5H2O (Ln: Ce and La).
Authors: An Haiyan , Xiao Dongrong , Wang Enbo , Li Yangguang , Wang Xinlong , Xu Lin 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Two New Hydrated Oxyhydroxides Sr3Co1.7Ti0.3O5 (OH)2×xH2O and Sr4Co1.6Ti1.4O8 (OH)2×xH2O Derived from the RP n = 2 and 3 Members: Structural and Magnetic Behavior versus Temperature.
Authors: Pelloquin D. , Barrier N. , Flahaut D. , Caignaert V. , Maignan A. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of 2‐Alkyl‐ and 2‐Aryl‐3‐aminopropionic Acids (β2‐Amino Acids) from (S)‐N‐Acryloyl‐5,5‐dimethyloxazolidin‐2‐one SuperQuat Derivatives.
Authors: Beddow James E. , Davies Stephen G. , Smith Andrew D. , Russell Angela J. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Reduction of Prochiral Ketones Using in situ Generated Oxazaborolidine Derived from (1S,2S,3R,4R)‐3‐Amino‐7,7‐dimethoxynorbornan‐2‐ol. An Efficient Synthesis of Enantiopure (R)‐Tomoxetine.
Authors: Lapis Alexandre A. M. , de Fatima Angelo , Martins Jose E. D. , Costa Valentim E. U. , Pilli Ronaldo A. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Preparation of Chiral 7,7′‐Disubstituted BINAPs for Rh‐Catalyzed 1,4‐Addition of Arylboronic Acids.
Authors: Yuan Wei‐Cheng , Cun Lin‐Feng , Gong Liu‐Zhu , Mi Ai‐Qiao , Jiang Yao‐Zhong 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Heterolytic Reactions of 2‐(Furyl‐2)‐1,3‐dioxacycloalkanes.
Authors: Chanysheva K. V. , Tokunova E. F. , Khusainov M. A. , Khlebnikova T. D. , Shakirova F. M. , Grishin S. A. , Kantor E. A. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reduction of Aldehydes and Oximes to Their Corresponding Alcohols and Amines by Catalytic Hydrogenation Method.
Authors: Basappa Basappa , Doreswamy B. H. , Mahendra M. , Mantelingu K. , Sridhar M. A. , Prasad J. Shashidhara , Rangappa K. S. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Active Palladium Catalysts Supported by Bulky Proazaphosphatrane Ligands for Stille Cross‐Coupling: Coupling of Aryl and Vinyl Chlorides, Room Temperature Coupling of Aryl Bromides, Coupling of Aryl Triflates, and Synthesis of Sterically Hindered Biaryls
Authors: Su Weiping , Urgaonkar Sameer , McLaughlin Patrick A. , Verkade John G. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Practical Synthesis of Optically Active Fluorine‐Substituted α‐Phenylethylamines by Retardation of Hydrogenolytic Cleavage at Benzylic Position.
Authors: Kanai Masatomi , Yasumoto Manabu , Kuriyama Yokusu , Inomiya Kenjin , Katsuhara Yutaka , Higashiyama Kimio , Ishii Akihiro 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Specific Features of Nucleophilic Substitution in 1‐Chloro‐3,4‐dinitrobenzene.
Authors: Zotova N. V. , Kushakova P. M. , Kuznetsov V. A. , Rodin A. A. , Garabadzhiu A. V. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereochemistry of the Reaction Catalyzed by 2‐Hydroxy‐6‐keto‐6‐phenyl‐hexa‐2,4‐dienoic Acid 5,6‐Hydrolase (BphD).
Authors: Li Jian‐Jun , Bugg Timothy D. H. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
An Environmentally Friendly Procedure for Mukaiyama Aldol and Mukaiyama—Michael Reactions Using a Catalytic Amount of DBU under Solvent‐ and Metal‐Free Conditions.
Authors: Shen Zhi‐Liang , Ji Shun‐Jun , Loh Teck‐Peng 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
1,3‐Dibromo‐5,5‐dimethylhydantoin [DBDMH] as an Efficient and Selective Agent for the Oxidation of Thiols to Disulfides in Solution or under Solvent‐Free Conditions.
Authors: Khazaei Ardeshir , Zolfigol Mohammad Ali , Rostami Amin 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Transformation of Schiff Bases Derived from α‐Naphthaldehyde. Synthesis, Spectral Data and Biological Activity of New 3‐Aryl‐2‐(α‐naphthyl)‐4‐thiazolidinones and N‐Aryl‐N‐[1‐(α‐naphthyl)but‐3‐enyl]amines.
Authors: Kouznetsov Vladimir , Rodriguez William , Stashenko Elena , Ochoa Carmen , Vega Celeste , Rolon Miriam , Montero Pereira David , Escario Jose A. , Gomez Barrio Alicia 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactions of Quadricyclane with Fluorinated Nitrogen‐Containing Compounds. Synthesis of 3‐Aza‐4‐perfluoroalkyl‐tricyclo[4.2.1.02,5]non‐3,7‐dienes.
Authors: Petrov Viacheslav , Davidson Frederic , Marshall Will 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Preparation and Stereochemistry of the Isopropylidene Derivatives of 1,2‐Dihydronaphtho[2,1‐b]furan‐1,2‐diol and 1,2,9,10‐Tetrahydronaphtho[2,1‐b:7,8‐b′]difuran‐1,2,9,10‐tetraol in Comparison with the Corresponding Acetyl Derivatives.
Authors: Yamaye Makoto , Motoyanagi Yoshiko , Nakagawa Aya , Kametani Sanae , Yoshinaga Tetsutaro , Cho Namiko , Fan Xiaobo , Kito Taketoshi 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Studies of Novel Synthetic Methodologies. Part 32. Amberlyst‐15 Catalyzed Acetylation of Heteroaromatics with Acetic Anhydride under Solvent Free Conditions.
Authors: Das Biswanath , Pal Rammohan , Banerjee Joydeep , Mahender G. , Holla Harish , Venkateswarlu K. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Studies on Reactions of Cyclic Oxalyl Compounds with Hydrazines or Hydrazones. Part 2. Synthesis and Reactions of 4‐Benzoyl‐1‐(4‐nitrophenyl)‐5‐phenyl‐1H‐pyrazole‐3‐carboxylic Acid.
Authors: Sener A. , Kasimogullari R. , Sener M. K. , Genc H. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
1,3,4‐Thiadiazoles. Regioselective O‐Demethylation on Dehydrative Cyclization of 1‐(3,4,5‐Trimethoxybenzoyl)‐4‐substituted Thiosemicarbazides with Sulfuric Acid.
Authors: Al‐Omar Mohamed , Al‐Deeb Omar A. , Al‐Khamees Hamad A. , El‐Emam Ali A. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 9‐Acridinyl Sulfur Derivatives: Sulfides, Sulfoxides and Sulfones. Comparison of Their Activity on Tumor Cells.
Authors: Santelli‐Rouvier Christiane , Barret Jean‐Marc , Farrell Christopher M. , Sharples Derek , Hill Bridget T. , Barbe Jacques 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Novel Synthetic Routes to New 3‐Substituted‐4‐oxo‐3,4,5,6,7,8‐hexahydropyrido[4′,3′,4,5]thieno [2,3‐d]pyrimidine‐7‐carboxylic Acid Ethyl Ester Derivatives.
Authors: Ahmed E. Kh. , Abdel‐latif F. F. , Ameen M. A. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthetic Approaches to Physiologically Active Polycyclic Compounds. Part 5. Ritter Reaction of 4‐Hydroxyadamantan‐2‐one.
Authors: Averina N. V. , Zefirova O. N. , Zefirov N. S. , Chekhlov A. N. , Shilov G. V. , Aldoshin S. M. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 2‐Aryl‐7‐(3‐oxo‐2H‐[1,4]‐benzoxazin‐6‐yl)pyrazolo[1,5‐a]pyrimidines as Potential COX‐2‐Inhibitors.
Authors: Reddy G. Jagath , Sailaja S. , Rao K. Srinivasa , Reddanna P. , Reddy D. Bharat 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Convenient Synthesis of New Purinyl‐homo‐carbonucleosides on a Cyclopentane Ring Fused with Pyridazine.
Authors: Caamano Olga , Gomez Generosa , Fernandez Franco , Garcia Marcos Daniel , Garcia‐Mera Xerardo , De Clercq Eric 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Guangsangons F—J, Antioxidant and Antiinflammatory Diels—Alder Type Adducts, from Morus macroura Miq.
Authors: Dai Sheng‐Jun , Ma Zhi‐Bo , Wu Yan , Chen Ruo‐Yun , Yu De‐Quan 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Electrochemical Studies of Biologically Important Systems
Authors: Yosypchuk Bogdan , Novotny Ladislav , Heyrovsky Michael , Sokolova Romana , Hromadova Magdalena , Pospisil Lubomir , Sestakova Ivana , Senholdova‐Dlaskova Zdenka , Navratil Tomas , Pristoupilova Kamila , et al. et al. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Monitoring proton dissociation and solution conformation of chiral ytterbium complexes with near‐IR CD
Authors: Lelli Moreno , Pintacuda Guido , Cuzzola Angela , Di Bari Lorenzo 
Published: April 2005

AbstractThe ytterbium complex [Yb((S)‐THP)]3+ ((S)‐THP = (1S,4S,7S,10S‐tetrakis(2‐hydroxypropyl)‐1,4,7,10‐tetraazacyclododecane) is investigated in solution through NMR, near‐IR absorption, and CD spectroscopy. Quantitative analysis of the paramagnetic pseudocontact NMR shift shows Λ helicity of the ligand cage around the metal. The NIR CD spectrum recorded at acidic pH is found to be very similar to that of [Yb((R)‐DOTMA)]− ((R)‐DOTMA = (1R,4R,7R,10R)‐α,α′,α′′,α′′′‐tetramethyl‐1,4,7,10‐tetraazacyclododecane‐1,4,7,10‐tetraacetic acid), which in solution assumes a twisted square antiprism (TSA) conformation. The similarity of the NIR CD spectra is discussed, and it is the first proof of the Λ(λ,λ,λ,λ) conformation of [Yb((S)‐THP)]3+. NIR CD spectra recorded in the pH range of 2–9 allow one to easily follow proton dissociation and to calculate the pK of this equilibrium in water (pKA = 6.4 ± 0.1). This value agrees well with that determined for [Lu((S)‐THP)]3+ using potentiometric methods. This demonstrates once again that NIR CD spectroscopy is a powerful technique for investigating the solution structure and dynamics of these complexes. Chirality 17:201–211, 2005. © 2005 Wiley‐Liss, Inc.
Determination of enantiomeric composition of (−)‐(R)‐2‐tert‐butyltetrahydroimidazolidin‐4‐one by polarimetry, 1H NMR, and chiral SFC
Authors: Garcia‐Martinez Cirilo , Hernandez Gilberto , Biba Mirlinda , Welch Christopher J. 
Published: April 2005

AbstractPartial resolution of rac‐2‐tert‐butyltetrahydroimidazolidin‐4‐one was carried out by recrystallization of diastereomeric salts. The enantiomeric composition of enriched samples was estimated by polarimetry, 1H NMR, and chiral SFC. Enantiomeric composition estimated by polarimetry or by 1H NMR was directly proportional to that estimated by chiral SFC. The occurrence of solute self‐association in chloroform was detected through measurements of optical and specific rotation at variable concentration of (−)‐(R)‐2‐tert‐butyltetrahydroimidazolidin‐4‐one. Our data suggest that solute self‐association in chloroform might be independent of enantiomeric composition. Chirality 17:212–217, 2005. © 2005 Wiley‐Liss, Inc.