bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluoroscopy-guided fine-needle aspiration of deep-seated pulmonary masses in dogs and cats appears safe and accurate
Authors: Jacob Frédéric 
Published: November 2023

Abstract

OBJECTIVE
Deep-seated pulmonary lesions can be difficult to sample safely. The objective of this study was to determine the relative safety and accuracy of fluoroscopy-guided fine-needle aspiration of deep-seated pulmonary lesions regardless of their size and depth.

ANIMALS
Client-owned animals; 5 dogs and 5 cats.

CLINICAL PRESENTATION
Pulmonary lesion locations were determined on dorsoventral and lateral views using fluoroscopy. The lateral thoracic wall was aseptically scrubbed, and an indelible marker was used to mark the point of entry of the needle for sampling. The path of a 22-gauge needle attached to a syringe was followed using fluoroscopic guidance. Mass volume (Vma) and distance from skin and pleura to lesion (DSK-L and DPL-L) were recorded.

RESULTS
In dogs, mean Vma was 137.2 cm3 (range, 6.3 to 426.2 cm3). Mean DSK-L was 71 mm (range, 37 to 101 mm) and DPL-L was 33 mm (range, 16 to 71 mm). Exfoliative cytology results were consistent with carcinoma in 4 dogs and lymphoma in 1 dog. A minor postprocedural complication was noted in 1 dog. In cats, the mean Vma was 2.4 cm3 (range, 1.6 to 3.7 cm3). Mean DSK-L was 42 mm (range, 20 to 75 mm) and DPL-L was 21 mm (range, 12 to 32 mm). Cytology results were consistent with pulmonary carcinoma in 2 cats, inflammation in 2 cats, and necrotic debris in 1 cat.

CLINICAL RELEVANCE
Fluoroscopy-guided fine-needle aspiration of pulmonary masses is a safe and accurate technique to obtain cytologic samples irrespective of the size and depth of the lesions.
БІНАРНІ ЗАНЯТТЯ У ПРОЦЕСІ РЕАЛІЗАЦІЇ ОНП “ПРАВО” В НАУКОВО-ДОСЛІДНОМУ ІНСТИТУТІ ВИВЧЕННЯ ПРОБЛЕМ ЗЛОЧИННОСТІ ІМЕНІ АКАДЕМІКА В.В. СТАШИСА НАПрН УКРАЇНИ
Authors: Батиргареєва Владислава , Ворожбіт-Горбатюк Вікторія , Євтєєва Дарина 
Published: November 2023

У статті автори презентували досвід організації і проведення бінарних занять для здобувачів третього освітньо-наукового рівня вищої освіти галузі знань 08 – право, спеціальності 081 – “право” у Науково-дослідному інституті вивчення проблем злочинності імені академіка В.В. Сташиса НАПрН України.
Описано досвід створення мотивуючого середовища для здійснення фундаментальних і прикладних досліджень для забезпечення прав і свобод людини, протидії злочинності, якісної підготовки фахівців для наукової і викладацької діяльності у науковій установі. У статті зазначено, що бінарне заняття має особливу поетапну організацію, інтегрований характер змісту і методики проведення. З опорою на висновки досліджень Z. Bekzhanova, K. Ryssaldy, C. Drinko виокремлено етапи бінарного заняття: організації, зосередження на цілях і очікуваних результатах, енергетичний.
Mach Number and Heating Effects on Turbulent Supersonic Jets
Authors: Georgiadis Nicholas J. , Wernet Mark P. , Locke Randy J. , Eck Dennis. G. 
Published: November 2023

Modern experimental techniques were used to obtain detailed aerodynamic and thermodynamic data for turbulent supersonic jets. Operating points from the work of Seiner et al. for heated Mach 2 jets were used as a starting point for the matrix of cases examined here using a geometrically similar Mach 2 nozzle. Two additional nozzles were examined having design Mach numbers of 1.36 and 1.63. For each Mach number, multiple jet temperatures were investigated. Jet measurements were first made with stagnation pressure and temperature probes for comparison to the previous experiments. The experiments focused on modern nonintrusive measurement techniques, including particle image velocimetry for mean and root-mean-square (rms) velocities, and rotationally resolved Raman scattering spectroscopy for mean and rms temperatures. Experimental results showed that as jet Mach number increases, the overall jet mixing rate decreases, as indicated by the potential core length, downstream mean velocities, and turbulence levels. Similar behavior was observed in the mean temperatures and rms temperatures. The suppression of turbulence as the jet Mach number increases is due to compressibility. For a fixed jet Mach number, increasing the jet temperature resulted in faster mixing by all measures. Peak turbulent stress levels, located along the lipline of the jet, demonstrated trends in individual stress components that differed depending on whether jet Mach number or temperature was varied, but indicated that the turbulence structure parameter [Formula: see text] was very similar when varying either jet temperature or Mach number.
Eagle syndrome: An updated review
Authors: Pagano Serena , Ricciuti Vittorio , Mancini Fabrizio , Barbieri Francesca Romana , Chegai Fabrizio , Marini Alessandra , Marruzzo Daniele , Paracino Riccardo , Ricciuti Riccardo Antonio 
Published: November 2023

Background:This work aims to review the current literature and our experience on vascular Eagle syndrome (ES) that can present misleading clinical presentations and better understand the possible therapeutic strategies.Methods:We reviewed the existing literature on PubMed from January 1, 2017, to December 31, 2022, including the sequential keywords “vascularANDEagle syndrome,” “vascularANDstyloid syndrome,” “vascularANDelongated styloid process,” “vascularANDstylocarotid syndrome,” and “Eagle syndromeANDcarotid artery dissection.”Results:38 vascular ES cases, including our experience, were analyzed. The most frequent clinical onset was hemiparesis (n21, 57%), but other regular clinical presentations were aphasia, loss of consciousness, amaurosis, headache, or a combination of the latter. Massive oral bleeding was reported only once in the literature before our case. Twelve patients were treated with only antiplatelet therapy, either single or double. Nine patients were treated with anticoagulation therapy only. In 14 patients, a carotid artery stent was used, associated with anticoagulation or antiplatelet therapy. In 17 cases, a styloid process (SP) resection was performed.Conclusion:ES has many clinical presentations, and carotid artery dissection resulting in oral bleeding seems rare. Literature results and our experience make us believe that when dealing with vascular ES, the best treatment strategy is endovascular internal carotid artery stenting with antiplatelet therapy, followed by surgical removal of the elongated SP to prevent stent fracture.
Impact of Secure Messaging Telehealth Service on the Quality of Health Care
Authors: Ko, PhD Dong-Gil 
Published: November 2023

Importance: Secure messaging is the only rapidly growing virtual care in the telehealth ecosystem; it is a cost-effective way to get medical advice and an alternative to an in-person, video, or telephone visit. Secure messaging has been shown to positively affect patients manage chronic diseases, improve health awareness and literacy, and improve communication and care coordination. However, providers have seen a 200% increase in secure messages in the past few years with some that are associated with medical visit avoidance and providers’ burnout. Such paradoxical tension raises questions about the value of secure messages and whether it impacts the quality of health care outcomes.
Objective: Conduct an empirical assessment and evaluate the extent to which and for whom secure messages improve the quality of health care.
Methods: Electronic health records of adult patients with type 2 diabetes mellitus with an active patient portal account who visited the Academic Medical Center between 2015-2020 were included in the analysis. Patients must have also initiated a secure message, made a follow-up in-person appointment, and completed the CAHPS (Consumer Assessment of Healthcare Providers and Systems) patient satisfaction survey.
Results: The final regression analysis included 1,332 patients with no missing data. The results reveal a negative relationship between patients who discuss their secure messages during their encounters and patient satisfaction even after controlling for patient-specific (age, gender, insurance), provider-specific (responsiveness), and medical conditions (average blood sugar, mean arterial pressure). The results also show a stronger negative effect for the underrepresented minorities (Blacks and Hispanics).
Discussion: The negative relationship between secure message exchanges and patient satisfaction may stem from different mental models between patients and providers as stocks of prior knowledge. Providers with their subject matter expertise have mental models that are incongruent with patients’ general health knowledge suggesting that the patients may struggle to recognize the value of new information, assimilate it, and apply it. On the other hand, effective communication is fundamental to patient satisfaction, and providers may be challenged to instill greater understanding during their average 18-minute encounters. The proliferation of digital divide research in health care indicates that Blacks and Hispanics are at a disadvantage; therefore, it is not unreasonable to suggests that the rate of health literacy differs possibly creating greater uncertainty and ambiguity. More research is needed that probes into the role and value of secure messaging as health care providers begin to charge patients for this telehealth service.
Modern possibilities of diagnostic and therapeutic interventions for decompensated liver cirrhosis: the focus is on «gut — liver — brain». Clinical case
Authors: Fadieienko G. D. , Nikiforova Y. V. 
Published: November 2023

The relevance of the issue of management of patients with decompensated liver cirrhosis (LC) is stipulated by its medical and socio­economic importance not only in Ukraine, but worldwide due to the high prevalence of etiological factors, as well as the development of severe complications that lead to fatal consequences. Chronic alcohol intoxication is considered the most common cause of LC. The understanding of the etiopathogenetic mechanisms of liver damage and the development of complications is constantly updated, which requires the improvement of approaches to the treatment of such patients. An important role in modern diagnostic and therapeutic interventions is given to the «gut/intestinal microbiota — liver — brain» axis. Today there is evidence that intestinal microbiota indirectly affects intestinal permeability, production of nitric oxide, release of biologically active peptides from enteroendocrine cells, production of short chain fatty acids — the main metabolic product of bacteria that feed cells of the mucous membrane, affect cognitive functions and the activation of the immune system. intestinal system. Research in recent years has proven that it is the liver that takes the leading place in the «gut/intestinal microbiota — liver — brain» axis system. Today, the pathogenesis of alcoholic liver disease is also associated with the hypothesis of «leakage» of the intestine, since alcohol significantly increases its permeability. Special attention in the development of encephalopathy with an imbalance of the microbiome is paid to bacterial translocation into the submucosa due to the violation of the integrity of the tight contacts of epithelial cells, with subsequent stimulation of the release of inflammatory mediators by smooth cells and lymphocytes, which leads to the development of the inflammatory process, increased vascular permeability in the portal, hepatic and systemic networks circulation.
The authors presented a clinical case of the use of modern possibilities of diagnostic and therapeutic interventions for decompensated liver cirrhosis of the of toxic etiology based on the global and domestic recommendations with a detailed discussion of patient care tactics.
ANALYSIS OF CLINICAL CRITERIA FOR ASSESSING QUALITY OF REMOVABLE ORTHOPEDIC APPLIANCES MANUFACTURED USING DOPED PACKAGING MATERIALS
Authors: Andrienko K.Yu. 
Published: November 2023

The purpose of this study is to analyse and compare clinical indicators for assessing the quality of removable dental prostheses manufactured using doped packing materials.
Materials and Methods. The study was carried out at the Department of Prosthetic Dentistry, University Dental Centre of Kharkiv National Medical University. We examined and treated 55 patients aged 45 to 75 years. The study involved clinical, biometric, macrohistochemical, and biomechanical assessments of the oral cavity; mathematical calculations were applied to analyze changes in the topographical and anatomical features of the jaws following the course of treatment.
Results. Achieving even distribution of chewing pressure from the prosthesis base to adjacent oral tissues is a primary goal in modern removable dental appliances that largely depends on the quality of the packaging material used for creating working plaster impression. Precisely replicating the microrelief of the oral mucosa on the prosthesis base improves fixation and stabilization, leading to reduced inflammation and tissue atrophy in the prosthetic area. A macrohistochemical study was used to objectively assess the clinical condition of the mucous membrane in the prosthetic area. After 6 months after the placement of removable denture, a significant reduction in the inflammation area was observed in the group, whose denture base was fabricated by using doped packaging materials, including KE-10-01 (1.8%), PVA (1.67%), BS-65-GP (1.62%), and PVA (1.1%). These findings indicate the functional benefits of using doped packaging materials in the first group of patients.
Conclusions. Statistical analysis of the findings obtained indicates that the atrophy of prosthetic area tissues was least pronounced in the control group. This underscores the effectiveness of utilizing doped packaging materials with optimized concentrations of KE-10-01, PVA, BS-65-GP, and PVA modifiers for manufacturing removable dental prostheses.
Linking Air Pollution Exposure to Blood-Based Metabolic Features in a Community-Based Aging Cohort with and without Dementia
Authors: Kalia Vrinda , Kulick Erin R. , Vardarajan Badri , Gu Yian , Manly Jennifer J. , Elkind Mitchell S.V. , Kaufman Joel D. , Jones Dean P. , Baccarelli Andrea A. , Mayeux Richard , Kioumourtzoglou Marianthi-Anna , Miller Gary W. 
Published: November 2023

Background: Long-term exposure to air pollution has been associated with changes in levels of metabolites measured in the peripheral blood. However, most research has been conducted in ethnically homogenous, young or middle-aged populations. Objective: To study the relationship between the plasma metabolome and long-term exposure to three air pollutants: particulate matter (PM) less than 2.5μm in aerodynamic diameter (PM2.5), PM less than 10μm in aerodynamic diameter (PM10), and nitrogen dioxide (NO2) in an ethnically diverse, older population. Methods: Plasma metabolomic profiles of 107 participants of the Washington Heights and Inwood Community Aging Project in New York City, collected from 1995–2015, including non-Hispanic white, Caribbean Hispanic, and non-Hispanic Black older adults were used. We estimated the association between each metabolic feature and predicted annual mean exposure to the air pollutants using three approaches: 1) A metabolome wide association study framework; 2) Feature selection using elastic net regression; and 3) A multivariate approach using partial-least squares discriminant analysis. Results: 79 features associated with exposure to PM2.5 but none associated with PM10 or NO2. PM2.5 exposure was associated with altered amino acid metabolism, energy production, and oxidative stress response, pathways also associated with Alzheimer’s disease. Three metabolites were associated with PM2.5 exposure through all three approaches: cysteinylglycine disulfide, a diglyceride, and a dicarboxylic acid. The relationship between several features and PM2.5 exposure was modified by diet and metabolic diseases. Conclusions: These relationships uncover the mechanisms through which PM2.5 exposure can lead to altered metabolic outcomes in an older population.
Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer
Authors: Li Danni , Li Xuran , Li Jiaojiao , Wang Yanhong , Tan Fei , Li Xiao 
Published: November 2023

Purpose: The combination of near-infrared (NIR) and positron emission tomography (PET) imaging presents an opportunity to utilize the benefits of dual-modality imaging for tumor visualization. Based on the observation that fibroblast activation protein (FAP) is upregulated in cancer-associated fibroblasts (CAFs) infiltrating all solid tumors, including head and neck squamous cell carcinoma (HNSCC), we developed the novel PET/NIR probe [68Ga]Ga-FAP-2286-ICG. Preclinically, the specificity, biodistribution and diagnostic properties were evaluated.Methods: Cell uptake assays were completed with the U87MG cell to evaluate the specificity of the [68Ga]Ga-FAP-2286-ICG. The tumor-targeting efficiency, biodistribution and optimal imaging time window of the [68Ga]Ga-FAP-2286-ICG were studied in mice bearing U87MG xenografts. HNSCC tumor-bearing mice were used to evaluate the feasibility of [68Ga]Ga-FAP-2286-ICG for tumor localization and guided surgical resection of HNSCC tumors.Results: The in vitro experiments confirmed that [68Ga]Ga-FAP-2286-ICG showed good stability, specific targeting of the probe to FAP, and the durable retention effect in high-expressing FAP tumors U87MG cell. Good imaging properties such as good tumor uptake, high tumor-to-background ratios (5.44 ± 0.74) and specificity, and tumor contouring were confirmed in studies with mice bearing the U87MG xenograft. PET/CT imaging of the probe in head and neck cancer-bearing mice demonstrated specific uptake of the probe in the tumor with a clear background. Fluorescence imaging further validated the value of the probe in guiding surgical resection and achieving precise removal of the tumor and residual lesions.Conclusion: In a preclinical model, these attractive [68Ga]Ga-FAP-2286-ICG PET/NIR imaging acquired in head and neck cancer make it a promising FAP-targeted multimodal probe for clinical translation.
The association between serum selenium levels and pathological features of papillary thyroid cancer in 284 patients
Authors: Ge Shenghui , Zhao Junyu , Yao Jinming , Fu Hang , Tian Yutian , Shan Yuanyuan , Sun Mengli , Feng Jing , Dong Jianjun , Liao Lin 
Published: November 2023

ObjectiveThe relationship between serum selenium levels and papillary thyroid cancer (PTC), especially the pathological features, still remains controversial. We conducted this study to investigate the relationship between serum selenium levels and PTC in a Chinese population.MethodsCross-sectional data of 284 patients with PTC were collected from the First Affiliated Hospital of Shandong First Medical University. The general clinical characteristics, serum selenium levels, and tumor pathological features were described in PTC. The association between serum selenium levels and pathological features in PTC was analyzed using SPSS 26.0 statistical software.ResultsOur results showed that the median serum selenium level was 79.15 μg/L (IQR: 71.00 - 86.98 μg/L) in PTC patients. Serum selenium levels were lower in females than males (p = 0.035). Serum selenium levels were negatively correlated with the number of lymph node metastases (p = 0.048). High serum selenium (OR = 0.397, 95%CI: 0.217 - 0.725) and diastolic blood pressure (OR = 1.028, 95%CI: 1.005 - 1.051) were related factors for the incidence of bilateral tumors. High serum selenium (OR = 0.320, 95%CI: 0.166 - 0.617) and diastolic blood pressure (OR = 1.066, 95%CI: 1.031 - 1.103) were related factors for tumor multifocal incidence.ConclusionsThe serum selenium levels of PTC patients in females were lower than males. High serum selenium levels might be a protective factor in PTC patients. Further research is necessary to better understand the influence of selenium on PTC progression.
A comprehensive approach to developing a multi-epitope vaccine against Mycobacterium tuberculosis: from in silico design to in vitro immunization evaluation
Authors: Jiang Fan , Han Yong , Liu Yinping , Xue Yong , Cheng Peng , Xiao Li , Gong Wenping 
Published: November 2023

IntroductionThe Bacillus Calmette-Guérin (BCG) vaccine, currently used against tuberculosis (TB), exhibits inconsistent efficacy, highlighting the need for more potent TB vaccines.Materials and methodsIn this study, we employed reverse vaccinology techniques to develop a promising multi-epitope vaccine (MEV) candidate, called PP13138R, for TB prevention. PP13138R comprises 34 epitopes, including B-cell, cytotoxic T lymphocyte, and helper T lymphocyte epitopes. Using bioinformatics and immunoinformatics tools, we assessed the physicochemical properties, structural features, and immunological characteristics of PP13138R.ResultsThe vaccine candidate demonstrated excellent antigenicity, immunogenicity, and solubility without any signs of toxicity or sensitization. In silico analyses revealed that PP13138R interacts strongly with Toll-like receptor 2 and 4, stimulating innate and adaptive immune cells to produce abundant antigen-specific antibodies and cytokines. In vitro experiments further supported the efficacy of PP13138R by significantly increasing the population of IFN-γ+ T lymphocytes and the production of IFN-γ, TNF-α, IL-6, and IL-10 cytokines in active tuberculosis patients, latent tuberculosis infection individuals, and healthy controls, revealing the immunological characteristics and compare the immune responses elicited by the PP13138R vaccine across different stages of Mycobacterium tuberculosis infection.ConclusionThese findings highlight the potential of PP13138R as a promising MEV candidate, characterized by favorable antigenicity, immunogenicity, and solubility, without any toxicity or sensitization.
Deletion of Salmonella enterica serovar Typhi tolC reduces bacterial adhesion and invasion toward host cells
Authors: Hussain Ashraf , Ong Eugene Boon Beng , Balaram Prabha , Ismail Asma , Kien Phua Kia 
Published: November 2023

BackgroundS. Typhi is a Gram-negative bacterium that causes typhoid fever in humans. Its virulence depends on the TolC outer membrane pump, which expels toxic compounds and antibiotics. However, the role of TolC in the host cell adhesion and invasion by S. Typhi is unclear.ObjectiveWe aimed to investigate how deleting the tolC affects the adhesion and invasion of HT-29 epithelial and THP-1 macrophage cells by S. Typhi in vitro.MethodsWe compared the adhesion and invasion rates of the wild-type and the tolC mutant strains of S. Typhi using in vitro adhesion and invasion assays. We also measured the expression levels of SPI-1 genes (invF, sipA, sipC, and sipD) using quantitative PCR.ResultsWe found that the tolC mutant showed a significant reduction in adhesion and invasion compared to the wild-type strain in both cell types. We also observed that the expression of SPI-1 genes was downregulated in the tolC mutant.DiscussionOur results suggest that TolC modulates the expression of SPI-1 genes and facilitates the adhesion and invasion of host cells by S. Typhi. Our study provides new insights into the molecular mechanisms of S. Typhi pathogenesis and antibiotic resistance. However, our study is limited by the use of in vitro models and does not reflect the complex interactions between S. Typhi and host cells in vivo.