bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant enterokinase light chain with affinity tag: Expression from Saccharomyces cerevisiae and its utilities in fusion protein technology
Authors: Choi Seong II , Song Hye Won , Moon Jae Woong , Seong Baik L. 
Published: August 2002

AbstractEnterokinase and recombinant enterokinase light chain (rEKL) have been used widely to cleave fusion proteins with the target sequence of (Asp)4‐Lys. In this work, we show that their utility as a site‐specific cleavage agent is compromised by sporadic cleavage at other sites, albeit at low levels. Further degradation of the fusion protein in cleavage reaction is due to an intrinsic broad specificity of the enzyme rather than to the presence of contaminating proteases. To offer facilitated purification from fermentation broth and efficient removal of rEKL after cleavage reaction, thus minimizing unwanted cleavage of target protein, histidine affinity tag was introduced into rEKL. Utilizing the secretion enhancer peptide derived from the human interleukin 1β, the recombinant EKL was expressed in Saccharomyces cerevisiae and efficiently secreted into culture medium. The C‐terminal His‐tagged EKL was purified in a single‐step procedure on nickel affinity chromatography. It retained full enzymatic activity similar to that of EKL, whereas the N‐terminal His‐tagged EKL was neither efficiently purified nor had any enzymatic activity. After cleavage reaction of fusion protein, the C‐terminal His‐tagged EKL was efficiently removed from the reaction mixture by a single passage through nickel‐NTA spin column. The simple affinity tag renders rEKL extremely useful for purification, post‐cleavage removal, recovery, and recycling and will broaden the utility and the versatility of the enterokinase for the production of recombinant proteins. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 718–724, 2001.
Personality features and characteristics of violent events committed by juvenile offenders
Authors: Loper Ann Booker , Hoffschmidt Sara Jane , Ash Eva 
Published: August 2002

AbstractThe present study investigated the relationship between characteristics of a violent event, as self‐reported by 82 incarcerated juvenile offenders, and personality features measured by the Millon Adolescent Clinical Inventory (MACI). The study predicted that specific personality features that have previously been associated with psychopathy would be associated with the instrumentality, emotional reactivity, and empathy or guilt reported for the incident. Results confirmed that a self‐reported pattern of elevated instrumental motivation and reduced empathy or guilt was associated with higher scores on the MACI Forceful, Unruly, Substance Abuse Proneness, Impulsive Propensity, and Family Discord Scales, as well as a recently developed Psychopathy Content Scale. Self‐reported elevated instrumental motivation and reduced empathy or guilt was also associated with lower scores on the Submissive, Conforming, Anxious Feelings, and Sexual Discomfort Scales. There were no significant relationships observed between emotional reactivity and personality scales. Copyright © 2001 John Wiley & Sons, Ltd.
Is it inherently prejudicial to try a juvenile as an adult?*
Authors: Levine Murray , Williams Andy , Sixt Amy , Valenti Robert 
Published: August 2002

AbstractGiven only information that a youth who could have been tried as either an adult or as a juvenile was being tried as an adult for murder, 218 undergraduate mock jurors were able to form consistent impressions of the defendant. A very high percent of our mock jurors included a criminal or juvenile justice history as part of that impression. A very large majority of the mock jurors also said that knowledge of that criminal history would be relevant to their vote of guilty. Almost all mock jurors said they would be influenced toward voting guilty by knowledge of a previous criminal history. Few of the other components of the impression were so closely correlated with a judgment of relevance, or with a judgment that they would be influenced toward voting guilty by the knowledge of that component of the stereotype. The effect is relatively specific to knowledge of a previous criminal history. The study has limited ecological validity. Nonetheless, we raise questions about whether the fact that a youth is put on trial as an adult is inherently prejudicial, and violates the Sixth Amendment right to an impartial jury. Copyright © 2001 John Wiley & Sons, Ltd.
Effect of paratenon and repetitive motion on the gliding resistance of tendon of extrasynovial origin
Authors: Momose Toshimitsu , Amadio Peter C. , Zobitz Mark E. , Zhao Chunfeng , An Kai‐Nan 
Published: August 2002

AbstractWe measured the gliding resistance of canine and human tendons of intrasynovial origin and tendons of extrasynovial origin with and without paratenon, and investigated the structure of paratenon using scanning electron microscopy. In the canine study, seven intrasynovial flexor digitorum profundus (FDP) tendons, seven extrasynovial fibularis (peroneus) longus (FL) tendons with paratenon, and seven FL tendons without paratenon were used. In the human study, seven intrasynovial FDP tendons and seven extrasynovial palmaris longus (PL) tendons cut in half (one half with paratenon, the other half without paratenon) were used. The gliding resistance of each tendon was measured at 1, 5, 10, 20, 50, and100 flexion/extension cycles. The canine and human FDP tendons maintained a gliding resistance significantly lower than that of the other tendons at all observation points (P < 0.05). In the canine, the gliding resistance of the FL tendon with paratenon was significantly lower than that of the FL tendon without paratenon up to 50 flexion/extension cycles (P < 0.05), but the two were not significantly different at 100 cycles. In the human, the gliding resistance of PL tendons with paratenon was significantly lower than that of the PL tendons without paratenon at all measuring points (P < 0.05). Preservation of paratenon thus appears to decrease the gliding resistance of extrasynovial tendons after repetitive motion in vitro. Clin. Anat. 15:199–205, 2002. © 2002 Wiley‐Liss, Inc.
Right superior septal artery with “normal” right coronary and ectopic “early” aortic origin: A contribution to the vascular supply of the interventricular septum of the human heart
Authors: Von Lüdinghausen Michael , Ohmachi Nobuko 
Published: August 2002

AbstractIn the human heart, anterior and posterior septal branches are mainly responsible for the arterial supply of the interventricular septum. These arteries are the basis of efficient intercoronary collateralization. The right and left superior septal arteries also contribute to the nourishment of the septum and to any eventual collateralization. Because the right superior septal artery (RSSA) is small in diameter, short, and has a variable origin either at the proximal stem or in the ostial area of the right coronary artery, it is difficult or almost impossible to visualize it angiographically. During investigation of the arterial supply of the interventricular septum in 84 human heart specimens and 16 corrosion casts, we found a few peculiarities in the origin and pattern of the RSSA in four specimens. The RSSA was found in 27 cases (27%); in most it was a single vessel and arose from three different locations: (a) the proximal part of the right coronary artery (21 cases); (b) the right coronary ostial area (four cases); and (c) from the floor of the right (anterior) aortic sinus (two cases). Macroscopically, in 16 cases the RSSA had a length of less than 10 mm; in nine cases the arteries were between 11 and 17 mm long. In two cases the RSSA was of more substantial appearance and up to 36 mm in length; it nourished almost the entire upper third of the septal myocardium. In these two cases, two courses could be differentiated: an extramural course with the RSSA descending to the subvalvular fibrous tissue, and an intramural course with ramification in the myocardium of the crista supraventricularis and the superior parts of the interventricular septum. One cadaveric heart specimen and one corrosion cast showed RSSAs that originated “early” (ectopically) on the floor of the right (anterior) aortic sinus; their total lengths were 16 and 17 mm, respectively. Such ectopic ostia of RSSAs have never been described before in the anatomical literature. Given the intense clinical concern with the identification of possible bypass vessels in the myocardium, we assume that the RSSA may have a potential as a collateral route. These findings were also discussed in light of developmental and comparative anatomy. Clin. Anat. 14:312–319, 2001. © 2001 Wiley‐Liss, Inc.
An additional renal vein
Authors: Dhar Pushpa 
Published: August 2002

AbstractA routine cadaveric dissection in an adult male revealed the emergence of two renal veins at the hilum of the right kidney that presented two separate openings in the inferior vena cava (IVC), one above the other. At the hilum the segmental branches of the right renal artery were sandwiched between the two veins. An additional venous tributary was present posterior to the right renal pelvis. A single renal vein emerged at the hilum of the left kidney. The various tributaries observed on the right and left sides were measured. The possible developmental basis for this variation suggests the persistence of the embryonic pattern observed in the 22 mm embryo. Clin. Anat. 15:64–66, 2002. © 2002 Wiley‐Liss, Inc.
Mental health and identity: the evaluation of a drop‐in centre
Authors: Hall Sarah , Cheston Richard 
Published: August 2002

AbstractThis paper is based on interviews with users of a drop‐in centre run by a voluntary group. Using a grounded theory approach the paper argues that individuals' use of the drop‐in is linked to the discursive strategies that they have developed to cope with stigma. Tajfel's Social Identity Theory is employed as a framework to examine how users manage the threat to their identity posed by the diagnosis and experience of mental illness. The implications of this research are examined within the context of the empowerment paradigm of mental health. Copyright © 2001 John Wiley & Sons, Ltd.
Diabetic patients treated with abciximab and intracoronary stenting
Authors: Walton Brian L. , Mumm Kim , Taniuchi Megumi , Kurz Howard I. , Lasala John M. 
Published: August 2002

AbstractDiabetic patients are at greater risk for restenosis, recurrent ischemia, and complications following angioplasty than are their nondiabetic counterparts. This is a retrospective study identifying diabetic patients who were treated with abciximab and intracoronary stenting during the period of January 1997 to December 1999. Abciximab was administered to 268 of 707 diabetic patients who received intracoronary stents from 1997 to 1999. The abciximab group contained a higher number of patients with severe ventricular dysfunction and high‐grade lesions. Primary endpoints of all‐cause mortality, same‐vessel revascularization, CABG, TVR, and postprocedural myocardial infarction were similar for both groups. The abciximab group had reduced rates of readmission for cardiac reasons during all follow‐up periods. The trends toward improvement of mortality, surgical or percutaneous revascularization, and cardiac readmissions suggest the effect of abciximab may provide benefit for up to 9 months for higher‐risk diabetic patients. Cathet Cardiovasc Intervent 2002;55:321–325. © 2002 Wiley‐Liss, Inc.
Successful balloon valvuloplasty in an adult patient with severe pulmonic stenosis and aneurysmal poststenotic dilatation
Authors: Fernandes Valerian , Kałuża Grzegorz L. , Zymek Pawet T. , DeFelice Clement A. , Hust Robert , Raizner Albert E. 
Published: August 2002

AbstractWe present a case of pulmonic stenosis with large aneurysmal poststenotic dilatation that was safely and effectively treated with balloon valvuloplasty. Though the poststenotic dilatation persists after the procedure, the risk of dissection and rupture is very low. Hence, balloon valvuloplasty should be considered the treatment of choice in this setting. Cathet Cardiovasc Intervent 2002;55:376–380. © 2002 Wiley‐Liss, Inc.
Endovascular stent implantation in the pulmonary arteries of infants and children without the use of a long vascular sheath
Authors: Pass Robert H. , Hsu Daphne T. , Garabedian Carl P. , Schiller Myles S. , Jayakumar K. Anitha , Hellenbrand William E. 
Published: August 2002

AbstractEndovascular stent implantation for pulmonary artery stenosis requires the use of a long, large‐bore vascular sheath to insure precise implantation without embolization or malposition. A long vascular sheath may be difficult to position and usage may be associated with vascular compromise and/or hemodynamic embarrassment, especially in infants and small children. We report a new technique for pulmonary artery endovascular stent implantation without the use of a long sheath. From December 2000 to May 2001, 10 patients underwent implantation of 13 Palmaz Corinthian premounted biliary transhepatic stents for pulmonary artery stenosis. Median age was 0.8 years (range, 0.5–18.5) and median weight was 11.8 kg (range, 4.6–65). Patient diagnoses were tetralogy of Fallot (five), double outlet right ventricle (three), branch peripheral pulmonary artery stenosis (two), single ventricle s/p cavopulmonary shunt (one), and truncus arteriosus (one). All Palmaz Corinthian stents were delivered uncovered on Cordis Opta LP balloon catheters via short sheaths (6–7 Fr); super‐stiff guidewires were not always necessary. These stents, with a maximal expanded diameter of 12 mm, were placed for peripheral pulmonary artery stenosis as a definitive procedure or at the pulmonary artery bifurcation in patients who were expected to undergo future open heart surgery. The stents were initially implanted on 4, 6, or 8 mm balloon catheters and further expanded if needed. Stents were placed in the right pulmonary artery alone in three patients, left pulmonary artery alone in four patients, and side‐by‐side stents were implanted simultaneously in three patients. All thirteen stents were implanted successfully in the desired location without stent malposition or embolization. Mean angiographic diameter increased from 2.5 ± 1.5 to 5.7 ± 1.4 mm (P < 0.01) and peak systolic ejection gradients decreased from 44 ± 22 to 14 ± 11.6 mm Hg (P < 0.01). The uncovered delivery of the premounted Palmaz Corinthian stent allowed for precise and safe endovascular stent implantation without the hemodynamic and technical problems associated with long vascular sheath usage. This technique is useful for the palliation of proximal pulmonary artery stenosis and is effective definitive treatment for peripheral pulmonary artery stenosis in small infants and children. Cathet Cardiovasc Intervent 2002;55:505–509. © 2002 Wiley‐Liss, Inc.
Inferior vena cava occlusion catheter for pediatric patients with heart disease: For more detailed cardiovascular assessments
Authors: Senzaki Hideaki , Miyagawa Katuya , Kishigami Yoshikazu , Sasaki Nozomu , Masutani Satoshi , Taketazu Mio , Kobayashi Jun , Kobyashi Toshiki , Asano Haruhiko , Kyo Shunei , Yokote Yuji 
Published: August 2002

AbstractTraditional evaluation of cardiac function is too often limited by reliance on measurements with complex interdependence between cardiac properties and loading factors. Analysis by ventricular pressure–volume (P–V), –area (P–A), or –dimension (P–D) relations during inferior vena caval (IVC) occlusion independently quantifies ventricular properties and loading conditions, providing detailed information about cardiovascular dynamics. However, there has been no appropriate size of balloon catheter that can effectively occlude IVC of pediatric patients, hindering the application of P–V (P–A, or P–D) analysis to children with heart disease despite its potential benefit. To address this problem, we have developed a new balloon catheter for IVC occlusion in children. The catheter effectively occluded IVC in 92 pediatric patients with varying forms of heart disease who underwent cardiac catheterization, yielding end‐systolic pressure–area relations. Thus a newly developed balloon catheter would contribute to establishing more accurate and detailed cardiovascular assessments in children with heart disease. Cathet Cardiovasc Intervent 2001;53:392–396. © 2001 Wiley‐Liss, Inc.
Closure of prosthetic paravalvular mitral regurgitation with the Gianturco‐Grifka vascular occlusion device
Authors: Eisenhauer Andrew C. , Piemonte Thomas C. , Watson Paul S. 
Published: August 2002

AbstractParavalvular regurgitation associated with prothetic mitral valves is often a consequence of infectious endocarditis. The condition is usually treated with debridement and repeat surgical valve replacement. However, repeated operations are associated with high risk. This report describes a case of successful transcatheter treatment of severe paravalvular mitral regurgitation and pulmonary edema in a patient in whom repeat mitral valve replacement was not believed possible. Cathet Cardiovasc Intervent 2001;54:234–238. © 2001 Wiley‐Liss, Inc.
The platelet function dose‐response to abciximab during percutaneous coronary revascularization is variable
Authors: Casterella Peter J. , Kereiakes Dean J. , Steinhubl Steven R. , Raymond Russell E. , Kottke‐Marchant Kandice , Patel Karen , Mueller Michelle , Rosenthal Monique , Moliterno David J. , Teirstein Paul S. 
Published: August 2002

AbstractThe platelet function dose‐response to incremental abciximab (Reopro, Eli Lilly/Centocor, Indianapolis, IN) bolus dosing during percutaneous coronary intervention (PCI) was evaluated in 85 patients using a point‐of‐service platelet function assay. Patients received incremental bolus doses of abciximab at 10‐ to 20‐min intervals; platelet function was measured at 10‐min intervals during dosing. The percentage of patients achieving ≥ 80% inhibition of platelet function after 50%, 75%, and 100% of a standard abciximab bolus was 40%, 87%, and 95%, respectively. There were no significant associations between the platelet function dose‐response to abciximab and age, weight, platelet count, hematocrit, heparin dose, peak activated clotting time, thienopyridine use prior to PCI, gender, cigarette smoking, diabetes mellitus, or clinical syndrome. This study demonstrated significant interpatient variability in platelet function dose‐response to abciximab with a substantial proportion (87%) of patients achieving high‐level platelet function inhibition with less than the standard abciximab bolus dose. Cathet Cardiovasc Intervent 2001;54:497–504. © 2001 Wiley‐Liss, Inc.
Selective coronary angiography in patients younger than 1 year of age
Authors: Butera Gianfranco , Saliba Zakhia , Aggoun Yacine , Bonnet Damien , Sidi Daniel , Kachaner Jean , Bonhoeffer Philipp 
Published: August 2002

AbstractPrecise assessment of coronary artery anatomy is needed in congenital coronary malformations and acquired coronary obstruction in children. The aim of the study was to describe our experience of selective coronary angiography (SCA) in newborns and infants. One hundred and three patients younger than 1 year of age underwent SCA. Indications were preoperative screening for coronary artery lesions or anomalous pattern (41 patients), signs of myocardial ischemia (43 patients), surgical difficulties during the reimplantation of the coronary arteries without signs of ischemia (15 patients), and other indications (4 patients). Success rate of selective injection was 100% with no significant complication. In the presence of clinical suspicion of myocardial ischemia, abnormalities of coronary artery pattern was found in 17 of 43 patients. Precious information for the surgical management was obtained in 13 of 41 patients. SCA can be performed safely even in newborns and small infants. Skills in catheterization and knowledge about the coronary artery anatomy are needed to perform SCA. Cathet Cardiovasc Intervent 2001;54:505–509. © 2001 Wiley‐Liss, Inc.
Intracoronary separation of a nosecone: An unusual complication of directional atherectomy
Authors: Suguta Masahiko , Nakano Akihiko , Hoshino Yoichi , Tomita Tomoyuki , Hasegawa Akira , Kurabayashi Masahiko 
Published: August 2002

AbstractA rare case of coronary separation of nosecone fixed to a 6 Fr GTO cutter catheter after failed directional coronary atherectomy (DCA) is reported. Revascularization was successful using a stent via the subintimal neolumen beside the nosecone. Heavily calcified lesions are relative contraindications to DCA. Cathet Cardiovasc Intervent 2001;54:526–530. © 2001 Wiley‐Liss, Inc.
Solubility, metastable zone width, and racemic characterization of propranolol hydrochloride
Authors: Wang Xin , Wang Xiu Juan , Ching Chi Bun 
Published: August 2002

AbstractCharacterization of the racemic species, which can be a racemic compound, a racemic conglomerate, or a pseudoracemate (solid solution), is a prerequisite for the design of crystallization resolution processes. It is useful to determine the solid/liquid equilibrium solubility of the enantiomer mixtures for crystallization operation. For the beta‐blocker drug propranolol hydrochloride, Gibbs free energy of formation of racemic compound and entropy of mixing of the (R)‐ and (S)‐ enantiomers in the liquid state for racemic conglomerate were calculated. The structural differences between (R, S)‐propranolol hydrochloride and its (S)‐enantiomer were further investigated by powder X‐ray diffraction patterns, infrared spectra, and solid‐state NMR spectra. The solubility and metastable zone width of (R, S)‐ propranolol hydrochloride in a mixed solvent of methanol and acetone were determined by cooling crystallization over the temperature range 3.5–42.5°C. The ternary solubility diagram of (R)‐, (S)‐propranolol hydrochloride was constructed using the same mixed solvent. The diagram will be useful as a guide for choosing crystallization operation conditions to produce pure enantiomers. Chirality 14:318–324, 2002. © 2002 Wiley‐Liss, Inc.
Enantiomeric separation of a thiazolbenzenesulfonamide compound using packed‐column subcritical fluid chromatography
Authors: Chen Lu , Thompson Richard A. , Johnson Bruce D. , Wyvratt Jean M. 
Published: August 2002

AbstractSeparation of enantiomers of a thiazolbenzenesulfonamide compound was performed on a Chiralpak® AD column using subcritical fluid chromatography. Effects of alcohol modifier and temperature on the separations were studied. The results revealed that while the main adsorbing interactions were between the hydroxyl group of the analyte and the carbamate group of the stationary phase, chiral discrimination was achieved through an inclusion mechanism within the chiral cavity created along the amylose chains. Analogs and synthetic precursors of the thiazolbenzenesulfonamide studied were also investigated so as to understand the effect of functional groups and configuration of the analyte molecule upon chiral recognition. Chirality 14:393–399, 2002. © 2002 Wiley‐Liss, Inc.
Chiroptical properties of binuclear rhodium complexes of lanostane alcohols
Authors: Frelek Jadwiga , Jagodziński Jacek , Meyer‐Figge Heike , Sheldrick William S. , Wieteska Edyta , Szczepek Wojciech J. 
Published: August 2002

AbstractThe circular dichroism (CD) of the in situ‐formed Rh2(OCOCF3)4 complexes of sterically hindered, secondary lanostane alcohols was investigated. The main object of the present studies are derivatives of 3β‐, 7α‐, 7β‐, and 11β‐hydroxylanostanes with or without an additional functional group, e.g., double bond, oxo‐, hydroxy‐, or acetoxy groups. Up to five Cotton effects (CEs) can be found in the CD spectra of Rh‐complexes of these alcohols in the spectral range between 650–300 nm. Correlation of the CEs signs with the absolute stereochemistry at the carbon atom bearing the hydroxy group was investigated. The Rh‐complex with the 3β‐acetoxylanostan‐11β‐ol ligand was isolated in the crystalline form. Its polymeric structure, determined by the X‐ray method, shows the di‐Rh‐units linked by the axially ligating oxygen atoms of the hydroxy and acetoxy groups. In the latter case, the coordination takes place through the carbonyl oxygen. Chirality 13:313–321, 2001. © 2001 Wiley‐Liss, Inc.