bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain Functional Connectivity and Anatomical Features as Predictors of Cognitive Behavioral Therapy Outcome for Anxiety in Youths
Authors: Zugman Andre , Ringlein Grace V. , Finn Emily S. , Lewis Krystal M. , Berman Erin , Silverman Wendy K. , Lebowitz Eli R. , Pine Daniel S. , Winkler Anderson M. 
Published: January 2024

AbstractBackgroundBecause pediatric anxiety disorders precede the onset of many other problems, successful prediction of response to the first-line treatment, cognitive-behavioral therapy (CBT), could have major impact. However, existing clinical models are weakly predictive. The current study evaluates whether structural and resting-state functional magnetic resonance imaging can predict post-CBT anxiety symptoms.MethodsTwo datasets were studied: (A) one consisted of n=54 subjects with an anxiety diagnosis, who received 12 weeks of CBT, and (B) one consisted of n=15 subjects treated for 8 weeks. Connectome Predictive Modeling (CPM) was used to predict treatment response, as assessed with the PARS; additionally we investigated models using anatomical features, instead of functional connectivity. The main analysis included network edges positively correlated with treatment outcome, and age, sex, and baseline anxiety severity as predictors. Results from alternative models and analyses also are presented. Model assessments utilized 1000 bootstraps, resulting in a 95% CI forR2,rand mean absolute error (MAE).OutcomesThe main model showed a mean absolute error of approximately 3.5 (95%CI: [3.1-3.8]) points aR2of 0.08 [−0.14 - 0.26] androf 0.38 [0.24 – 0.511]. When testing this model in the left-out sample (B) the results were similar, with a MAE of 3.4 [2.8 – 4.7],R2−0.65 [−2.29 – 0.16] androf 0.4 [0.24 – 0.54]. The anatomical metrics showed a similar pattern, where models rendered overall lowR2.InterpretationThe analysis showed that models based on earlier promising results failed to predict clinical outcomes. Despite the small sample size, the current study does not support extensive use of CPM to predict outcome in pediatric anxiety.
Proximity labeling proteomics reveals Kv1.3 potassium channel immune interactors in microglia
Authors: Bowen Christine A , Nguyen Hai M , Lin Young , Bagchi Pritha , Natu Aditya , Espinosa-Garcia Claudia , Werner Erica , Kumar Prateek , Tobin Brendan R , Wood Levi , Faundez Victor , Wulff Heike , Seyfried Nicholas T , Rangaraju Srikant 
Published: January 2024

ABSTRACTMicroglia are the resident immune cells of the brain and regulate the brain’s inflammatory state. In neurodegenerative diseases, microglia transition from a homeostatic state to a state referred to as disease associated microglia (DAM). DAM express higher levels of proinflammatory signaling, like STAT1 and TLR2, and show transitions in mitochondrial activity toward a more glycolytic response. Inhibition of Kv1.3 decreases the proinflammatory signature of DAM, though how Kv1.3 influences the response is unknown. Our goal was to establish the potential proteins interacting with Kv1.3 during the TLR4-mendiated transition to DAM. We utilized TurboID, a biotin ligase, fused to Kv1.3 to evaluate the potential interacting proteins with Kv1.3 via mass spectrometry in BV-2 microglia during an immune response. Electrophysiology, western blots, and flow cytometry were used to evaluate Kv1.3 channel presence and TurboID biotinylation activity. We hypothesized that Kv1.3 contains domain-specific interactors that vary during an TLR4-induced inflammatory response, some of which are dependent on the PDZ-binding domain on the C-terminus. We determined that the N-terminus of Kv1.3 is responsible for trafficking Kv1.3 to the cell surface and mitochondria (e.g.NUNDC, TIMM50). The C-terminus interacts with immune signaling proteins in an LPS-induced inflammatory response (e.g.STAT1, TLR2, and C3). There are 70 proteins that rely on the c-terminal PDZ-binding domain to interact with Kv1.3 (i.e.ND3, Snx3, and Sun1). Overall, we highlight that the Kv1.3 potassium channel functions beyond outward flux of potassium in an inflammatory context and contributes to activity of key immune signaling proteins, such as STAT1 and C3.MAIN POINTSKv1.3 channels are highly abundant in pro-inflammatory microglia in neurological diseases. Kv1.3 channels may regulate microglial functions by interacting with other proteins via its N and C terminal domains.Using proximity-based proteomics, we identified several novel proteins that interact with the N and C terminus of Kv1.3 channels, some of which are domain-specific.Kv1.3 channels in microglia interact with many immune signaling proteins, including Tlr2, Stat1 and integrins.Under homeostatic conditions, the N-terminus of Kv1.3 interacts with proteins involved in protein trafficking, to the cell surface and mitochondria. The PDZ-binding region was an important determinant of the C terminal interactome.During an LPS-induced inflammatory response, the C-terminus of Kv1.3 uniquely interacts with immune and signaling proteins of disease relevance, including STAT1
Physcomitrium patensflavodiiron proteins form a redox-dependent heterocomplex
Authors: Beraldo Claudia , Traverso Eleonora , Boschin Marco , Cendron Laura , Morosinotto Tomas , Alboresi Alessandro 
Published: January 2024

AbstractFlavodiiron proteins (FLVs) catalyze the reduction of oxygen to water by exploiting electrons from Photosystem I (PSI). In several photosynthetic organisms such as cyanobacteria, green algae, mosses and gymnosperms, FLV-dependent electron flow protects PSI from over-reduction and consequent damage especially under fluctuating light conditions. In this work we investigated biochemical and structural properties of FLVA and FLVB from the model mossPhyscomitrium patens. The two proteins, expressed and purified fromEscherichia coli, bind both iron and flavin cofactors and show NAD(P)H oxidase activity as well as oxygen reductase capacities. Moreover, the co-expression of both FLVA and FLVB, coupled to a tandem affinity purification procedure with two different affinity tags, enabled the isolation of the stable and catalytically active FLVA/B hetero multimer protein complex, that has never been isolated and characterized so far. The multimeric organization was shown to be stabilized by inter-subunit disulfide bonds. This investigation provides valuable new information on the biochemical properties of FLVs, with new insights into theirin vivorole and regulation.
Large structural variants in KOLF2.1J are unlikely to compromise neurological disease modelling
Authors: Ryan Mallory , McDonough Justin A. , Ward Michael E. , Cookson Mark R. , Skarnes William C. , Merkle Florian T. 
Published: January 2024

SummaryGracia-Diaz and colleagues analysed high-density DNA microarray and whole genome sequencing (WGS) data from the KOLF2.1J ‘reference’ human induced pluripotent stem cell (hiPSC) line1, and report the presence of five high-confidence heterozygous copy number variants (CNVs) at least 100kbp in length2. Since three of these CNVs span coding genes, some of which have been associated with neurodevelopmental disease, the authors raise the concern that these CNVs may compromise the utility of KOLF2.1J for neurological disease modelling. We appreciate their thorough analysis and thoughtful interpretation, and agree that potential users of this line should be made aware of all cases where KOLF2.1J differs from the reference genome. However, we believe that the benefits from the widespread use of KOLF2.1J outweigh the potential risks that might arise from the identified CNVs.
Creating something out of nothing: Symbolic and non-symbolic representations of numerical zero in the human brain
Authors: Barnett Benjy , Fleming Stephen M. 
Published: January 2024

SummaryRepresenting the quantity zero as a symbolic concept is considered a unique achievement of abstract human thought. Despite considerable progress in understanding the neural code supporting natural numbers, how numerical zero is encoded in the human brain remains unknown. We find that both non-symbolic empty sets (the absence of dots on a screen) and symbolic zero (‘0’) occupy ordinal positions along graded neural number lines within posterior association cortex. Neural representations of zero are partly independent of numerical format, exhibiting distance effects with countable numerosities in the opposing (symbolic or non-symbolic) format. Our results show that format-invariant neural magnitude codes extend to judgements of numerical zero and help motivate theoretical accounts in which representations of symbolic zero are grounded in more basic representations of sensory absences.
Does actors' engagement with capacity-building training programs enable delivery of SDG-aligned public services? The case of senior public officials
Authors: Azmat Fara , Ferdous Ahmed Shahriar , Wali Faisal , Muttakin Mohammad Badrul , Haque Mohammed Ziaul 
Published: January 2024

PurposeThis study examines whether engagement with Sustainable Development Goal (SDG)-focused specialized training programs enable senior public officials (focal actor) to collectively deliver on public services that have a transformational societal impact over time. Further, the study explores the factors that impede and facilitate the delivery of such services. The authors do so by using service mechanics theorization and drawing on the lens of actor and collective engagement.Design/methodology/approachThis study undertakes a longitudinal exploratory qualitative study design. SDG-focused training programs were delivered, as interventions, for two cohorts of senior public officials from Bangladesh in an Australian University in 2017 and 2019. In-depth interviews were conducted upon the training's completion and then after 8- and 12-month intervals to assess the short- and long-term impact respectively.FindingsAn empirical framework is proposed from the study findings. It shows that engagement – cognitive, emotional and behavioral – with SDG-focused specialized training programs enables focal actors (i.e. senior public officials) to engage other actors (other public officials, community members) in networks, facilitated the delivery of SDG-aligned public services. Such engagement results in a transformative impact that spans micro (individual), meso (organizational) and macro (societal) levels over time. Factors that impede and facilitate SDG-aligned delivery of public services are also identified.Research limitations/implicationsTheoretically, the authors contribute to the literature that relates to actor and collective engagement, SDG-focused capacity-building training programs and service mechanics. Practically, this study informs organizations about the ways that they can effectively engage their senior employees with capacity-building training programs that focus on sustainability.Originality/valueThis study is one of the few that connects the interface between public service delivery for enacting societal changes and SDG-focused capacity-building training programs through service mechanics theorization and using the lens of actor and collective engagement.
A phenomenological study of university lecturers’ EFL teaching challenges in an Asian setting
Authors: Sipe Marlon , Segumpan Reynaldo Gacho 
Published: January 2024

PurposeEnglish, the lingua franca of the world, permeates a global teaching-learning space not only for communication per se but also as a medium of instruction and social interaction. The authors studied the lived experiences of five English-speaking university lecturers as regards English as a foreign language (EFL) teaching in a non-English-speaking Asian country.Design/methodology/approachUsing a phenomenological inquiry and through Zoom interviews, the authors studied the lived experiences of five English-speaking university lecturers as regards EFL teaching in a non-English-speaking Asian country.FindingsThematic analyses guided the authors' understanding of the participants’ lived experiences in the classroom, such as the following: (1) Local students have a negative perception of EFL; (2) lecturers’ professional standards are hardly imposed and (3) local students have a mixed level of English proficiency. The authors also examined the participants’ lived experiences in the organization, where the following issues surfaced: (1) sending of communication messages beyond working hours, (2) bureaucratic working environment and (3) changing of policies.Practical implicationsThe authors' research implies the need for programs and policies that will help EFL teachers deal with instructional and other socio-cultural challenges in an Asian context.Originality/valueIn the Asian setting, there is a dearth of literature that focuses on the experience of university lecturers of English as a Foreign Language (EFL), in particular, their teaching challenges. The authors confronted this gap by understanding the local beliefs about EFL teaching among English-speaking university lecturers.
Gen Z buying patterns: comparing the influence of professional advising and social media engagement
Authors: Stoica Michael , Hickman Thomas M. 
Published: January 2024

Purpose
This paper aims to assess retail shopping avenues for young consumers based on the type of guidance and assistance they pursue (social media vs professional service provider) in purchasing situations judged to be important. Young consumers consider themselves pet parents and pets represent an extension of self. Thus, pet-related products, by their importance, represent an excellent area of analysis for this inquiry.

Design/methodology/approach
Data collection was completed in a way that is contextually relevant and gives the results ecological validity with the assistance of a major supplier of pet food. A total of 470 Gen Z owners of small companion animals were obtained. The partial least squares structural equation modeling testing method is considered appropriate for the model analysis due to the structure and homogeneity of the sample and due to the precision of the method.

Findings
Results indicate that, in accordance with social media engagement (SME) theory, high SME elevated social commerce purchase intentions while professional advice seekers reported increased brick-and-mortar shopping. Thus, context-dependent, young consumers may purchase from brick-and-mortar sites.

Originality/value
This paper draws on SME and Advice Response Theory and proposes an original model explaining channel selection preferences for young consumers based on the type of advice sought for important purchasing decisions. The model represents a steppingstone to investigate the mechanism that translates information search sources into buying through different channels.
Constraints of digestion on swimming performance and stress tolerance vary with habitat in freshwater fish species
Authors: ZHANG Yongfei , LUO Yulian , HUANG Keren , LIU Qianying , FU Cheng , PANG Xu , FU Shijian 
Published: January 2024

AbstractLimited aerobic scope (AS) during digestion might be the main constraint on the performance of bodily functions in water‐breathing animals. Thus, investigating the postprandial changes in various physiological functions and determining the existence of a shared common pattern because of possible dependence on residual AS during digestion in freshwater fish species are very important in conservation physiology. All species from slow‐flow habitats showed impaired swimming speed while digesting, whereas all species from fast‐flow habitats showed strong swimming performance, which was unchanged while digesting. Only two species from slow‐flow habitats showed impaired heat tolerance during digestion, suggesting that whether oxygen limitation is involved in the heat tolerance process is species‐specific. Three species from slow‐ or intermediate‐flow habitats showed impaired hypoxia tolerance during digestion because feeding metabolism cannot cease completely under hypoxia. Overall, there was no common pattern in postprandial changes in different physiological functions because: (1) the digestion process was suppressed under oxygen‐limiting conditions, (2) the residual AS decreased during digestion, and (3) performance was related to residual AS, while digestion was context‐dependent and species‐specific. However, digestion generally showed a stronger effect on bodily functions in species from slow‐flow habitats, whereas it showed no impairment in fishes from fast‐flow habitats. Nevertheless, the postprandial change in physiological functions varies with habitat, possibly due to divergent selective pressure on such functions. More importantly, the present study suggests that a precise prediction of how freshwater fish populations will respond to global climate change needs to incorporate data from postprandial fishes.
Exercise‐inducible circulating extracellular vesicle irisin promotes browning and the thermogenic program in white adipose tissue
Authors: Shi Hongwei , Hao Xiaojing , Sun Yaqin , Zhao Yating , Wang Yue , Cao Xiaorui , Gong Zeen , Ji Shusen , Lu Jiayin , Yan Yi , Yu Xiuju , Luo Xiaomao , Wang Juan , Wang Haidong 
Published: January 2024

AbstractAimExercise can reduce body weight and promote white fat browning, but the underlying mechanisms remain largely unknown. This study investigated the role of fibronectin type III domain‐containing protein 5 (FNDC5)/Irisin, a hormone released from exercising muscle, in the browning of white fat in circulating extracellular vesicles (EVs).MethodsMice were subjected to a 4 weeks of running table exercise, and fat browning was analyzed via histology, protein blotting and qPCR. Circulating EVs were extracted by ultrahigh‐speed centrifugation, and ELISA was used to measure the irisin concentration in the circulating EVs. Circulating EVs that differentially expressed irisin were applied to adipocytes, and the effect of EV‐irisin on adipocyte energy metabolism was analyzed by immunofluorescence, protein blotting, and cellular oxygen consumption rate analysis.ResultsDuring sustained exercise, the mice lost weight and developed fat browning. FNDC5 was induced, cleaved, and secreted into irisin, and irisin levels subsequently increased in the plasma during exercise. Interestingly, irisin was highly expressed in circulating EVs that effectively promoted adipose browning. Mechanistically, the circulating EV‐irisin complex is transported intracellularly by the adipocyte membrane receptor integrin αV, which in turn activates the AMPK signaling pathway, which is dependent on mitochondrial uncoupling protein 1 to cause mitochondrial plasmonic leakage and promote heat production. After inhibition of the AMPK signaling pathway, the effects of the EV‐irisin on promoting fat browning were minimal.ConclusionExercise leads to the accumulation of circulating EV‐irisin, which enhances adipose energy metabolism and thermogenesis and promotes white fat browning in mice, leading to weight loss.
Tumor‐derived Prevotella intermedia aggravates gastric cancer by enhancing Perilipin 3 expression
Authors: Liang Wei , Zhou Zhengyang , Gao Qizhao , Zhu Zhichen , Zhu Jie , Lin Jiayao , Wen Yicheng , Qian Feinan , Wang Liang , Zhai Yaxuan , Lv Jingnan , Zhang Haifang , Zhong Fengyun , Du Hong 
Published: January 2024

AbstractThe indigenous microbial milieu within tumorous tissues exerts a pivotal influence on the genesis and advancement of gastric cancer (GC). This investigation scrutinizes the functions and molecular mechanisms attributed to Prevotella intermedia in the malignant evolution of GC. Isolation of P. intermedia from paired GC tissues was undertaken. Quantification of P. intermedia abundance in 102 tissues was accomplished using quantitative real‐time PCR (qRT‐PCR). Assessment of the biological effects of P. intermedia on GC cells was observed using culture medium supernatant. Furthermore, the protein profile of GC cells treated with tumor‐derived P. intermedia was examined through label‐free protein analysis. The functionality of perilipin 3 (PLIN3) was subsequently confirmed using shRNA. Our investigation revealed that the relative abundance of P. intermedia in tumor tissues significantly surpassed that of corresponding healthy tissues. The abundance of P. intermedia exhibited correlations with tumor differentiation (p = 0.006), perineural invasion (p = 0.004), omentum majus invasion (p = 0.040), and the survival duration of GC patients (p = 0.042). The supernatant derived from tumor‐associated P. intermedia bolstered the proliferation, clone formation, migration, and invasion of GC cells. After indirect co‐cultivation with tumor‐derived P. intermedia, dysregulation of 34 proteins, including PLIN3, was discerned in GC cells. Knockdown of PLIN3 mitigated the malignancy instigated by P. intermedia in GC cells. Our findings posit that P. intermedia from the tumor microenvironment plays a substantial role in the malignant progression of GC via the modulation of PLIN3 expression. Moreover, the relative abundance of P. intermedia might serve as a potential biomarker for the diagnosis and prognosis of GC.
The (patho)physiological roles of the individual deacylase activities of a sirtuin
Authors: Zheng Weiping 
Published: January 2024

AbstractSince the discovery of the sirtuin family founding member (i.e., the yeast silent information regulator 2 (sir2) protein) in 2000, more and more sirtuin proteins have been identified and are currently known to be present in organisms from all the three kingdoms of life (i.e., bacteria, archaea, and eukarya). Seven sirtuin proteins have been identified in mammals including humans, that is, SIRT1/2/3/4/5/6/7. Sirtuin proteins are a class of enzymes with primary catalytic activity being the β‐nicotinamide adenine dinucleotide (β‐NAD+ or NAD+)‐dependent deacylation from the Nε‐acyl‐lysine residues on cellular proteins. Many sirtuins (e.g., human SIRT1/2/3/4/5/6/7) have been found to each possess multiple individual deacylase activities acting on Nε‐acyl‐lysine substrates with different acyl groups ranging from the simple formyl and acetyl to the more complex groups like succinyl and myristoyl; however, our current knowledge on the (patho)physiological roles of these individual deacylase activities is still limited, which could be due to the currently still thin research toolbox for investigation (i.e., the deacylase‐selective sirtuin mutant and inhibitor/activator). In this article, an updated account on the subject matter will be presented with biochemical and medicinal chemistry perspectives.