bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential lineage restriction of rat retinal progenitor cells in vitro and in vivo
Authors: Yang Peng , Seiler Magdalene J. , Aramant Robert B. , Whittemore Scott R. 
Published: October 2002

AbstractTo identify and characterize the lineage potential of rat neural retina progenitor cells (NRPCs) in vitro and engrafted into rats with retinal degeneration, NRPCs were isolated from neural retinas of embryonic day 17 Long Evans rats and cultured in serum‐free or serum‐containing media with fibroblast growth factor 2 and neurotrophin 3. After expansion, cellular differentiation was initiated by the withdrawal of these growth factors. Despite forming primary neurospheres, NRPCs cultured in serum‐free medium survived poorly after passage. In contrast, NRPCs cultured in serum‐containing medium could be expanded for up to 12 passages and differentiated into glial fibrillary acidic protein‐positive glial cells and retina‐specific neurons expressing rhodopsin, S‐antigen, calbindin, recoverin, and calretinin. For in vivo analysis, passage 1 (P1) undifferentiated NRPCs were labeled with bromodeoxyuridine (BrdU), implanted into the subretinal space of Royal College of Surgeons (RCS) rats, and analyzed immunohistochemically 4 weeks postgrafting. The grafted NRPCs showed extensive glial differentiation, irrespective of their topographic localization. A few BrdU‐labeled grafted NRPCs expressed protein kinase C, a marker for bipolar and amacrine interneuron‐specific differentiation. Other retina‐specific or oligodendrocytic differentiation was not detected in the grafted cells. Although NRPCs are capable of self‐renewal and multilineage differentiation in vitro, they developed mostly into glial cells following engraftment into the adult retina. These data suggest that the adult retina retains epigenetic signals that are either restrictive for neuronal differentiation or instructive for glial differentiation. Induction of lineage‐specific cell differentiation of engrafted NRPCs to facilitate retinal repair will likely require initiation of specific differentiation in vitro prior to grafting and/or modification of the host environment concomitantly with NRPC grafting. © 2002 Wiley‐Liss, Inc.
Self‐esteem, appraisal and coping: a comparison of unemployed and re‐employed people
Authors: Waters Lea E. , Moore Kathleen A. 
Published: October 2002

AbstractThe negative effects of unemployment on psychological health are well documented, yet Kasl's (1982) reverse causation hypothesis is that positive psychological health, and in particular self‐esteem, facilitates re‐employment. The aim of this study was to investigate this proposal by assessing levels of self‐esteem, cognitive appraisals and coping efforts among unemployed persons and relating these factors to their employment status six months later. Two hundred and one unemployed (49 per cent female, 51 per cent male; mean age = 32.41 ± 10.18 years) and 128 employed respondents (59 per cent female, 41 per cent male; mean age = 35.0 ± 11.73 years) participated in the study. Participants completed the Adult Self‐Perception Profile, Access to Categories of Experience, Locus of Control, Deakin Coping Scale, and the Meaningful Leisure Activities Questionnaire at baseline and at six‐month follow‐up using a mail‐out survey. Comparison of baseline appraisals revealed that future re‐employed participants rated their latent deprivation lower and their internal locus of control higher than those continuously unemployed, and they also derived more internal meaning from leisure activities. Overall, the results provide support for Kasl's reverse causation hypothesis extended to these other domains of psychological health. Intervention strategies designed to incorporate the promotion of these factors are discussed. Copyright © 2002 John Wiley & Sons, Ltd.
Man‐made climatic changes in the Ganges basin
Authors: Adel Miah M. 
Published: October 2002

AbstractClimate data pertaining to the Ganges basin in Bangladesh were analysed to find any climatic changes in the wake of the upstream water diversion by the Farakka Barrage. Whereas the diversions have been continuing from at least 30 international rivers upstream of Bangladesh, the diversion from the Ganges is the best known and has a wider coverage than all other diversions. The diversion reduced the Ganges' discharge through the delta by about 60% from a pre‐diversion average value of 1932 m3 s−1, decreased water availability in flood plains, ponds, canals, and ditches by about 50%, dropped the groundwater table, and caused changes in surface features. It took about 5 years of diversions beyond the test run year of 1975 for the environment to react to set 1981 as the baseline year. During the post‐baseline era: (1) heating degree days and cooling degree days were respectively 1.33 and 1.44 times more than their counterparts during the pre‐baseline era; (2) the summertime and wintertime average temperatures were respectively 1 °C more and 0.5 °C less than the corresponding values during the pre‐baseline era; (3) the mode 32 °C of summertime maximum temperatures was 1 °C higher and occurred 414 times more, and the mode 25 °C of wintertime temperature was 1 °C less and occurred 17 times less than the corresponding quantities during the pre‐baseline era; (4) the average value of maximum relative humidity has increased by more than 2% and that of minimum relative humidity has dropped by the same amount; (5) the mode 95% and 70% of maximum and minimum relative humidity values have occurred 1322 times and 84 times more respectively than their pre‐baseline counterparts; and (6) the frequency for 100 mm or more rainfall and the monthly average rainfalls have dropped by about 50% and 30% respectively. The solution to the climatic changes lies in the restoration of the virgin Ganges flow, dredging of the Ganges and its distributaries to remove shoals and siltation, and re‐excavation of canals for water discharge to depleted surface water bodies to re‐establish the lost wetland ecosystems. The findings are useful for climate modellers to predict the climatic changes due to changes in surface features, for policy makers of governments of riparian countries constructing dams/barrages on international rivers for unilateral diversion of water, and for donor agencies who finance such projects. Copyright © 2002 Royal Meteorological Society.
Precipitation and the North Atlantic Oscillation: a study of climatic variability in northern England
Authors: Fowler H. J. , Kilsby C. G. 
Published: October 2002

AbstractVariations in precipitation in Yorkshire, UK, are linked to large‐scale atmospheric circulation using the North Atlantic Oscillation (NAO) index, objective Lamb weather types, and long‐term seasonal and annual precipitation indices at seven sites. Since the 1960s, a significant decrease in summer precipitation has been observed across the region, with increasing winter precipitation at western locations since 1970. At western sites, precipitation is highly correlated to the NAO, with positive and negative winter‐NAO indices producing anomalies from the long‐term winter precipitation average of 112% and 77% respectively. The recent winter half‐year precipitation increase is concentrated in March and related to a large increase in monthly NAO and the increased occurrence of westerly weather types. The decrease in summer precipitation, however, is caused by both declining receipt from westerly weather types and their decreased occurrence. This is significantly correlated to a decreasing summer index for the NAO. The NAO, the frequency of weather types and precipitation totals are all intimately linked on an annual and monthly time scale. Strong connections between winter precipitation and the NAO may prove very important, particularly in western Yorkshire where most surface water supplies are located. If the NAO can be predicted using sea‐surface temperature, it may allow the estimation of winter reservoir replenishment in western Yorkshire a few months in advance. This may provide an important predictor of future hydrological drought episodes and facilitate forward planning and management of water resource systems located in climatologically similar regions of the UK. Copyright © 2002 Royal Meteorological Society
The climatology of sea breezes on Sardinia
Authors: Furberg M. , Steyn D. G. , Baldi M. 
Published: October 2002

AbstractHourly wind speed and direction data from 12 coastline stations on Sardinia, Italy, are analysed in order to characterize sea breezes in the region. A set of criteria based on the diurnal reversal of wind direction, and the thermal gradient necessary to drive the circulation, is used to identify sea breeze days. Statistics are presented that describe the occurrence, duration, and strength of the sea breezes. On a stationwide basis, sea breezes are most frequent in the summer months (May–August), when they appear on more than one‐third of the days. Sea breeze occurrence and duration are the greatest for the stations on the east coast of the island. The all‐station average sea breeze duration reaches a maximum of about 9 h in June. The strength of the sea breezes is roughly 3 m s−1 during summer months on average over all stations in the sample.An analysis of mean daily hodographs for the stations in the sample shows clearly the onshore–offshore nature of the sea breeze circulation, and the response of the sea breezes to the local coastline. Sea breezes are shown to develop simultaneously on all coasts of the island under appropriate synoptic conditions. Copyright © 2002 Royal Meteorological Society.
Structured water in hydrotalcites of formula MgxZn6−xAl2(OH)16(CO3)·4H2O: a Raman microscopic study
Authors: Johnson Therese E. , Martens Wayde , Frost Ray L. , Ding Z. , Theo Kloprogge J. 
Published: October 2002

AbstractRaman microscopy was used to characterize synthesized hydrotalcites of formula MgxZn6−xAl2(OH)16 (CO3)·4H2O. The Raman spectra are conveniently subdivided into spectral features based on (a) the carbonate anion, (b) the hydroxyl units and (c) metal–oxygen units. A model is proposed based on a tripod of M3OH units in the hydrotalcite structure. In a simplified model, Raman spectra of the hydroxyl‐stretching region enable bands to be assigned to the Mg3OH, Zn3OH and Al3OH units. Bands are also assigned to the hydroxyl stretching vibrations of water. Three types of water are identified: (a) water hydrogen bonded to the interlayer carbonate ion, (b) water hydrogen bonded to the hydrotalcite hydroxyl surface and (c) interlamellar water. A model of water in the hydrotalcite structure is proposed in which water is in a highly ordered structure as it is hydrogen bonded to both the carbonate anion and the hydroxyl surface and also forms bridges between the MOH surface and the carbonate anion. Copyright © 2002 John Wiley & Sons, Ltd.
Characterisation of northwest Moroccan honeys by gas chromatographic–mass spectrometric analysis of their sugar components
Authors: Terrab Anass , Vega‐Pérez José M , Díez María J , Heredia Francisco J 
Published: October 2002

AbstractCombined gas chromatography–mass spectrometry (GC–MS) was used to analyse the sugar composition of honeys from northwest Morocco. This technique allowed the identification and quantification of two monosaccharides, eight disaccharides and three trisaccharides. In addition, characterisation of the main unifloral honeys by principal component analysis (PCA) and stepwise discriminant analysis (SDA) was carried out. PCA showed that the cumulative variance was approximately 50%, and about 70% of the samples were correctly classified using SDA, with the best results being obtained for the honeydew honeys (100% correct).© 2001 Society of Chemical Industry
Lipid analysis—a 20th century success?
Authors: Hammond Eugene W 
Published: October 2002

AbstractLipid analysis, providing an understanding of chemistry and structure, begins in Continental Europe during the 18th century. However, it necessarily takes until the mid 20th century before methodology develops sufficiently for the production of fast accurate and quantitative data. Up to 1922 analytical methodology relies on good observation and painstaking care, but only achieves bulk properties. Key early workers are Tachen and Scheele, followed by the great Chevreul, while Pelouze and Berthelot later added to his work. Thus by 1900 we had knowledge of the basic structure and composition of fat, enabling Lewkowitsch to publish manuals of data and simple analytical methodology in 1901 and 1922.Hilditch, in the UK from 1925 to 1952, much improved analytical methods and knowledge, leading to publication of the major work, The Chemical Constitution of Natural Fats. In 1951 Martin and James published the key development of gas chromatography. It led to rapid advancement of knowledge in structure, chemistry and biochemistry of lipid. Parallel and future develoments in thin layer and high performance liquid chromatography complemented this. Other developments, in particular NMR and mass spectrometry, have created an exciting completion to the success of 20th century lipid analysis.© 2001 Society of Chemical Industry
Enzymic debittering of Indian grapefruit (Citrus paradisi) juice
Authors: Prakash S , Singhal R S , Kulkarni P R 
Published: October 2002

AbstractThe present work explores the use of enzymes for debittering grapefruit (Citrus paradisi) juice. The debittering process was optimised using a statistical approach. A full factorial design using enzyme concentration (0.25, 0.5 and 1.0 g l−1), incubation temperature (25, 30 and 40 °C) and time of incubation (1, 2 and 4 h) as variables gave a regression equation that could predict the extent of debittering. The optimum set of conditions recommended is an enzyme concentration of 1 g l−1 at 40 °C for 4 h. The extent of naringin breakdown achieved under these conditions was 75%.© 2002 Society of Chemical Industry
Cell wall composition of 1B/1R translocation wheat grains
Authors: Selanere Mangala L , Andersson Roger 
Published: October 2002

AbstractThe cell wall composition and total dietary fibre content of 12 different winter wheat cultivars both without and with 1B/1R gene were analysed. Translocation wheat (with 1B/1R gene) had higher contents of extractable xylose and unextractable mannose residues than standard wheat (without 1B/1R gene). Three sieved flours of standard wheat, translocation wheat and rye respectively were used for further detailed studies. Water‐extractable arabinoxylan was isolated and fractionated on a DEAE‐cellulose column. The water‐eluted fraction of translocation wheat had a higher proportion of monosubstituted xylose residues than the same fraction of standard wheat, which is a typical feature of rye arabinoxylan. Molecular weight determination showed that rye water‐eluted arabinoxylans were larger than those of wheat. The molecular weight of translocation wheat arabinoxylan was lower than that of standard wheat and had a high polydispersity. Xylanase‐resistant arabinoxylan was extracted from all three flours, and NMR studies showed that wheat xylanase‐resistant arabinoxylan had a lower amount of monosubstituted xylose residues, while the rye fraction was rich in consecutive disubstituted xylose residues. Translocation wheat xylanase‐resistant arabinoxylan resembled that of standard wheat.© 2002 Society of Chemical Industry
Analysis of the theaflavin composition in black tea (Camellia sinensis) for predicting the quality of tea produced in Central and Southern Africa
Authors: Wright Louwrance P , Mphangwe Nicholas I K , Nyirenda Hastings E , Apostolides Zeno 
Published: October 2002

AbstractA parameter of black tea that correlates with quality is highly desired. Twenty good and 20 poor quality tea clones were selected from the breeding programme at the Tea Research Foundation (Central Africa) (TRF(CA)). The theaflavin (TF) composition of the black tea manufactured from each clone was analysed by capillary electrophoresis and correlated with total scores and valuation from two tea tasters with regression analysis. The significance of the differences between the 20 good and 20 poor quality tea clones was determined with the Student t‐test and the distribution‐free Kruskal–Wallis test. The sum of the TFs of the black tea correlated well (r = 0.785, P = 0.0002) with the value of the tea. Of the individual TFs determined in the black tea, the highest correlation was obtained with TF‐3′‐monogallate (TF‐B) (r = 0.788, P = 0.0016), TF‐3‐monogallate (TF‐A) (r = 0.741, P = 0.0004) and free TF (TF‐f) (r = 0.705, P = 0.0002). TF‐B (m = 24.90) followed by TF‐A (m = 12.48) showed the steepest slopes against value, making them good indicators for value. The TF‐digallate (TF‐dg) content did not show significant correlation with value (r = 0.316, P = 0.358). The individual TF content thus can be used for optimising the black tea manufacturing process in Central and Southern Africa.© 2002 Society of Chemical Industry
Oxidation of polyphenols in unfermented and partly fermented cocoa beans by cocoa polyphenol oxidase and tyrosinase
Authors: Misnawi  , Selamat Jinap , Bakar Jamilah , Saari Nazamid 
Published: October 2002

AbstractUnfermented and partly fermented dried cocoa beans have an excessively astringent and bitter flavour owing to their high polyphenol content. Studies on the remaining polyphenol oxidase activity and the oxidation of polyphenols in these beans have been conducted in relation to two factors, ie incubation time (0, 1, 2, 4, 8 and 16 h) and pH of incubation (3.5, 4.5, 5.5 and 6.5). Owing to unsuccessful polyphenol oxidation during incubation of partly fermented beans, incubation–enrichment treatments were carried out by combining incubation time (0, 8, 16, 24 and 32 h) and addition of crude cocoa polyphenol oxidase and purified tyrosinase from mushroom at 88 and 8800 units g−1 and pH 5.5. Results showed that the remaining polyphenol oxidase activities of unfermented and partly fermented dried beans were 1 and 0.08% with specific activities of 9 and 1% respectively. The polyphenols of unfermented beans were effectively oxidised by incubation at 45 °C and pH 3.5–6.5 without enzyme enrichment; while those of partly fermented beans required enzyme enrichment. Both crude cocoa polyphenol oxidase and tyrosinase could be used for enzyme enrichment, but tyrosinase seemed to be more effective.© 2002 Society of Chemical Industry
Optimisation of kojic acid monolaurate synthesis with lipase PS from Pseudomonas cepacia
Authors: Chen Chee‐Shan , Liu Kuan‐Ju , Lou Ya‐Hui , Shieh Chwen‐Jen 
Published: October 2002

AbstractTo improve the instability of kojic acid in food and cosmetic use, the esterification of kojic acid catalysed by lipase from Pseudomonas cepacia (Amano PS) to synthesise kojic acid monolaurate (KAML) was investigated in this study. Response surface methodology (RSM) with a five‐level/five‐factor central composite rotatable design (CCRD) was employed to evaluate the effects of synthesis parameters such as reaction time (8–24 h), temperature (35 55 °C), enzyme amount (10–50%), substrate molar ratio of lauric acid to kojic acid (1:1–3:1) and added water content (0–20%) on the percentage molar conversion to KAML by direct esterification. Reaction time and added water content were the most important variables, while substrate molar ratio had less effect on percentage molar conversion. Based on canonical analysis and ridge maximum analysis, optimal synthesis conditions were reaction time 19 h, temperature 44 °C, enzyme amount 38%, substrate molar ratio 2:1 and added water content 10%. The predicted value was 85% and the actual experimental value 82% molar conversion.© 2002 Society of Chemical Industry
Muscle protein solubility of some cephalopods (pota and octopus) during frozen storage
Authors: Moral A , Morales J , Ruíz‐Capillas C , Montero P 
Published: October 2002

AbstractThis study relates to myofibrillar and sarcoplasmic protein solubility in 5% NaCl and collagen solubility in 0.05 M acetic acid, from muscle and mantle of pota (Todaropsis eblanae) and octopus (Eledone cirrhosa) kept in frozen storage for 12 months. In both species, protein solubility in 5% NaCl remained high, over 60%, throughout the storage period. Solubility was lower in the octopus than in the pota lots. The species also behaved differently in terms of the increase in solubility, which occurred earlier in octopus (after 2 months) than in pota (after 5–6 months). Thereafter, solubility gradually declined. The values for solubility of proteins from mantle of both pota and octopus in 5% NaCl were greater in young specimens than in mature samples. There was no clear gender‐dependent tendency. The acid solubility of collagen also increased in most lots up to the sixth month, after which it characteristically began to decline. Pota collagen was significantly more soluble in mantles than in arms. In octopus mantle, collagen solubility gradually declined. In the sexually developed phases (immature and mature), no gender‐dependent differences were detected in the solubility of collagen from octopus mantle and arms. However, values did tend to be slightly higher in females. The mantles of immature specimens of pota and octopus were more suitable for freezing.© 2002 Society of Chemical Industry
Physical and chemical properties of chufa (Cyperus esculentus L) tubers grown in the Çukurova region of Turkey
Authors: Coşkuner Yalçın , Ercan Recai , Karababa Erşan , Nazlıcan Ahmet Nedim 
Published: October 2002

AbstractFour lines of chufa (Cyperus esculentus) grown in 1998 and 1999 in the Çukurova region of Turkey were analysed for their physical properties, proximate chemical composition and fatty acid contents. The chufa lines contained on average (g kg−1) 932.8 dry matter, 245.0 crude lipid, 256.8 starch, 14.3 ash, 50.5 protein, 89.1 crude fibre, 17.1 reducing sugar, 154.3 total sugar and 130.4 sucrose. Hunter L, a+ and b+ colour values of ground chufa samples were in the ranges 55.93–60.59, 3.71–5.09 and 15.60–16.85 respectively. Individual chufa tubers weighed between 0.224 and 0.283 g. The fatty acid composition of chufa oil included (g kg−1) 689.2–732.9 oleic acid, 125.5–141.2 palmitic acid and 99.6–154.6 linoleic acid, which is comparable with that of olive oil. After storage for 1 year the differences in mean values were significant (p < 0.05).© 2002 Society of Chemical Industry
Feasibility of fishmeal replacement by shrimp head silage protein hydrolysate in Nile tilapia (Oreochromis niloticus L) diets
Authors: Plascencia‐Jatomea Maribel , Olvera‐Novoa Miguel A , Arredondo‐Figueroa José Luis , Hall George M , Shirai Keiko 
Published: October 2002

AbstractThis study provides information on the use of shrimp head silage protein hydrolysate (SPH) as an alternative protein source for tilapia feeding. Six diets (28% protein, 12% lipid) were prepared where fishmeal protein was replaced at levels of 0 (control), 10, 15, 20, 25 and 30% with the hydrolysate. The diets were supplied to Nile tilapia fry (338 mg initial weight) stocked in plastic recirculating 20 l tanks (10 animals per tank), with three replicates per treatment. After an 8 week experimental period, fish fed the diets containing 10 and 15% SPH showed significantly better performance in terms of final body weight, weight gain (%), mean daily weight gain (mg day−1), specific growth ratio and feed conversion ratio than those fed the control diet (fishmeal as protein source) and higher‐SPH diets. It is concluded that shrimp head hydrolysate is a promising alternative protein source for tilapia feeding, improving growth ratio at dietary inclusion levels as high as 15%. In addition, the diets with added shrimp silage protein were well accepted by the fish, which avidly consumed the feed during the experiment.© 2002 Society of Chemical Industry