bluuebunny/crossref_metadata_2025_split huggingface dataset:
The 15-min (Sub)Cellular Proteome
Authors: Shen Bowen , Pade Leena R. , Nemes Peter 
Published: February 2024

AbstractSingle-cell mass spectrometry (MS) opens a proteomic window onto the inner workings of cells. Here, we report the discovery characterization of the subcellular proteome of single, identified embryonic cells in record speed and molecular coverage. We integrated subcellular capillary microsampling, fast capillary electrophoresis (CE), high-efficiency nano-flow electrospray ionization, and orbitrap tandem MS. In proof-of-principle tests, we found shorter separation times to hinder proteome detection using DDA, but not DIA. Within a 15-min effective separation window, CE data-independent acquisition (DIA) was able to identify 1,161 proteins from single HeLa-cell-equivalent (∼200 pg) proteome digests vs. 401 proteins by the reference data-dependent acquisition (DDA) on the same platform. The approach measured 1,242 proteins from subcellular niches in an identified cell in the liveXenopus laevis(frog) embryo, including many canonical components of organelles. CE-MS with DIA enables fast, sensitive, and deep profiling of the (sub)cellular proteome, expanding the bioanalytical toolbox of cell biology.Authorship ContributionsP.N. and B.S. designed the study. L.R.P. collected theX. laeviscell aspirates. B.S. prepared and measured the samples. B.S. and P.N. analyzed the data and interpreted the results. P.N. and B.S. wrote the manuscript. All the authors commented on the manuscript.
Long-term adaptation of prefrontal circuits in a mouse model of NMDAR hypofunction
Authors: Ponserre Marion , Ionescu Tudor M , Franz Alessa A , Deiana Serena , Schuelert Niklas , Lamla Thorsten , Williams Rhîannan H , Wotjak Carsten T , Hobson Scott , Dine Julien , Omrani Azar 
Published: February 2024

AbstractPharmacological approaches to induce N-methyl-D-aspartate receptor (NMDAR) hypofunction have been intensively used to understand the aetiology and pathophysiology of schizophrenia. Yet, the precise cellular and molecular mechanisms that relate to brain network dysfunction remain largely unknown. Here, we used a set of complementary approaches to assess the functional network abnormalities present in mice that underwent a 7-day subchronic phencyclidine (PCP 10mg/kg, subcutaneously, once daily) treatment. Our data revealed that pharmacological intervention with PCP affected cognitive performance and auditory evoked gamma oscillations in the prefrontal cortex (PFC) mimicking endophenotypes of some schizophrenia patients. We further assessed PFC cellular function and identified altered neuronal intrinsic membrane properties, reduced parvalbumin (PV) immunostaining and diminished inhibition onto L5 PFC pyramidal cells. A decrease in the strength of optogenetically-evoked glutamatergic current at the ventral hippocampus (HPC) to PFC synapse was also demonstrated, along with a weaker shunt of excitatory transmission by local PFC interneurons. On a macrocircuit level, functional ultrasound measurements indicated compromised functional connectivity within several brain regions particularly involving PFC and frontostriatal circuits. Herein, we reproduced a panel of schizophrenia endophenotypes induced by subchronic PCP application in mice. We further recapitulated electrophysiological signatures associated with schizophrenia and provided an anatomical reference to critical elements in the brain circuitry. Together, our findings contribute to a better understanding of the physiological underpinnings of deficits induced by subchronic NMDAR antagonist regimes and provide a test system for characterization of pharmacological compounds.HighlightsSubchronic PCP treatment alters cognitive performance and evoked gamma synchronization in the prefrontal cortex.Subchronic PCP reduces number of parvalbumin-positive boutons and inhibitory synaptic transmission onto pyramidal neurons in layer 5/6 of the prelimbic cortex.Subchronic PCP reduces the strength of ventral hippocampal inputs to the medial prefrontal cortex.Frontostriatal circuits show robust dysconnectivity after subchronic PCP treatment. Abstract
Cortical population coding critically depends on fine-tuning of cell physiology
Authors: Revah Omer , Wolf Fred , Gutnick Michael J. , Neef Andreas 
Published: February 2024

AbstractCortical function reflects the activities of populations of neurons. How accurately they can encode information depends on the speed with which each neuron can respond to input, as revealed by dynamic gain analysis. Theory predicts that the determinants of a population’s dynamic gain - cell number, cell size and the correlation time of the background noise - control the speed with which the population can respond to input. Because the constituents of a neuronal population change from moment to moment, experimental confirmation of these predictions has not been feasible. Here, we study a stable, defined neuronal population with known input and connectivity: spiny stellate cells in layer 4 of rodent barrel cortex. We confirm predictions as to the impacts of cell size and input correlation time and we demonstrate that these disparate factors are precisely matched with the number of neurons in layer 4, such that even a single thalamocortical spike at the input is reliably reflected in the population output. We further show that the speed with which the spike-generation machinery can respond is modulated by the channels that mediate M-current, suggesting that coding in layer 4 may vary as a function of brain state.One-Sentence SummaryIn layer 4 of the somatosensory cortex, the neuronal population is able to perform its precise temporal function because it is tuned by a set of disparate parameters.
Ion-channel degeneracy and heterogeneities in the emergence of signature physiological characteristics of dentate gyrus granule cells
Authors: Kumari Sanjna , Narayanan Rishikesh 
Published: February 2024

ABSTRACTComplex systems are neither fully determined nor completely random. Biological complex systems, including single neurons, manifest intermediate regimes of randomness that recruit integration of specific combinations of functionally segregated subsystems. Such emergence of biological function provides the substrate for the expression of degeneracy, the ability of disparate combinations of subsystems to yield similar function. Here, we present evidence for the expression of degeneracy in morphologically realistic models of dentate gyrus granule cells (GC) through functional integration of disparate ion-channel combinations. We performed a 45-parameter randomized search spanning 16 active and passive ion channels, each biophysically constrained by their gating kinetics and localization profiles, to search for valid GC models. Valid models were those that satisfied 17 sub- and supra-threshold cellular-scale electrophysiological measurements from rat GCs. A vast majority (>99%) of the 15,000 random models were not electrophysiologically valid, demonstrating that arbitrarily random ion-channel combinations wouldn’t yield GC functions. The 141 valid models (0.94% of 15,000) manifested heterogeneities in and cross-dependencies across local and propagating electrophysiological measurements, which matched with their respective biological counterparts. Importantly, these valid models were widespread throughout the parametric space and manifested weak cross-dependencies across different parameters. These observations together showed that GC physiology could neither be obtained by entirely random ion-channel combinations nor is there an entirely determined single parametric combination that satisfied all constraints. The complexity, the heterogeneities in measurement and parametric spaces, and degeneracy associated with GC physiology should be rigorously accounted for, while assessing GCs and their robustness under physiological and pathological conditions.GRAPHICAL ABSTRACT
TLR9 activation in large wound induces tissue repair and hair follicle regeneration via γδT cells
Authors: Li Xinhui , Yang Yang , Yi Zumu , Xu Zhaoyu , Chen Shuaidong , An Tiantian , Zhou Feng , Deng Chen , Man Yi , Hu Chen 
Published: February 2024

AbstractThe mechanisms underlying tissue repair in response to damage have been one of main subjects of investigation. In this study, we leveraged the wound-induced hair neogenesis (WIHN) models in adult mice to explore the inner correlation. Our investigation revealed that heightened release of mitochondrial DNA (mtDNA) accompanying tissue damage activated the toll-like receptor 9 (TLR9) pathway, influencing the repair process and the ultimate number of regenerated hair follicles. Furthermore, our analysis of single-cell RNA sequencing comparisons demonstrated increased TLR9 activation was associated with the recruitment of gamma delta T cells (γδT). Inhibition of γδT cell recruitment led to a reduction in the population of γδT cells and a more fibrotic healing outcome. Notably, these γδT cells exhibited distinctive high production of AREG, contributing to the rapid increase of local AREG levels around the epidermis and influencing the fate commitment of keratinocytes. These findings provide new insights into the roles of TLRs as critical mediators in the sense of tissue damage, the modulation of immune cell activity, and the ultimate influence on healing outcomes.TeaserStarting with how tissue injury stimulates downstream tissue repair and regeneration through relevant signals, this study explored the phenomenon and correlation between tissue damage and TLR9, and the effect of TLR9 on γδT, keratinocytes and the healing outcomes.
Licensing and competition of stem cells at the niche combine to regulate tissue maintenance
Authors: García-Tejera Rodrigo , Amoyel Marc , Grima Ramon , Schumacher Linus 
Published: February 2024

AbstractTo maintain and regenerate adult tissues after injury, the numbers, proliferation, and differentiation rates of tissue-resident stem cells must be precisely regulated. The regulatory strategies preventing exhaustion or overgrowth of the stem cell pool, whether there is coordination between different mechanisms, and how to detect them from snapshots of the cell populations, remains un-resolved. Recent findings in the Drosophila testes show that prior to differentiation, somatic stem cells transition to a state that licenses them to differentiate upon receiving a commitment signal, but remain capable of fully regaining stem cell function. Here, we build stochastic mathematical models for the somatic stem cell population to investigate how licensing contributes to homeostasis and the variability of stem cell numbers. We find that licensing alone is sufficient regulation to maintain a stable homeostatic state and prevent stem cell extinction. Comparison with previous experimental data argues for the likely presence of regulation through competition for niche access. We show that competition for niche access contributes to the reduction of the variability of stem cell numbers but does not prevent extinction. Our results suggest that a combination of both regulation strategies, licensing and competition for niche access, is needed to reduce variability and prevent extinction simultaneously.
Pleotropic effects of a recessive COL1α2mutation occurring in a mouse model of severe osteogenesis imperfecta
Authors: Corcelli Michelangelo , Sagar Rachel , Petzendorfer Ellen , Hasan Mohammad Mehedi , de Vries Hilda I , van Dijk Fleur S , David Anna L , Guillot Pascale V 
Published: February 2024

AbstractApproximately 85-90% of individuals with Osteogenesis Imperfecta (OI) have dominant pathogenic variants in theCOL1A1 or COL1A2genes. This leads to decreased or abnormal Collagen type I production. Subsequently, bone formation is strongly reduced, causing bone fragility and liability to fractures throughout life. OI is clinically classified in 5 types with the severity ranging from mild to lethal depending on the gene and the type and location of the OI-causative variant and the subsequent effect on (pro) collagen type I synthesis. However, the specific effects on the phenotype and function of osteoblasts are not fully understood.To investigate this, the OI murine model was used, with theoim/oim(OIM) mice closest resembling severely deforming OI type 3 in humans. We showed that in OIM, COL1 mutation results in a multifactorial inhibition of the osteogenic differentiation and maturation as well as inhibition of osteoclastogenesis. The phenotype of differentiated OIM osteoblasts also differs from that of wild type mature osteoblasts, with upregulated oxidative cell stress and autophagy pathways, possibly in response to the intracellular accumulation of type I collagen mRNA. The extracellular accumulation of defective type I collagen fibres contributes to activation of the TGF-β signalling pathway and activates the inflammatory pathway. These effects combine to destabilise the balance of bone turnover, increasing bone fragility. Together, these findings identify the complex mechanisms underlying OI bone fragility in the OIM model of severe OI and can potentially enable identification of clinically relevant endpoints to assess the efficacy of innovative pro-osteogenic treatment for patients with OI.
The crystal structure of mycothiol disulfide reductase (Mtr) provides mechanistic insight into the specific low-molecular-weight thiol reductase activity of Actinobacteria
Authors: Gutiérrez-Fernández Javier , Hersleth Hans-Petter , Hammerstad Marta 
Published: February 2024

Low-molecular-weight (LMW) thiols are involved in many processes in all organisms, playing a protective role against reactive species, heavy metals, toxins and antibiotics. Actinobacteria, such as Mycobacterium tuberculosis, use the LMW thiol mycothiol (MSH) to buffer the intracellular redox environment. The NADPH-dependent FAD-containing oxidoreductase mycothiol disulfide reductase (Mtr) is known to reduce oxidized mycothiol disulfide (MSSM) to MSH, which is crucial to maintain the cellular redox balance. In this work, the first crystal structures of Mtr are presented, expanding the structural knowledge and understanding of LMW thiol reductases. The structural analyses and docking calculations provide insight into the nature of Mtrs, with regard to the binding and reduction of the MSSM substrate, in the context of related oxidoreductases. The putative binding site for MSSM suggests a similar binding to that described for the homologous glutathione reductase and its respective substrate glutathione disulfide, but with distinct structural differences shaped to fit the bulkier MSSM substrate, assigning Mtrs as uniquely functioning reductases. As MSH has been acknowledged as an attractive antitubercular target, the structural findings presented in this work may contribute towards future antituberculosis drug development.
Bolstering circular economy in construction through digitalisation
Authors: Eze Emmanuel Chidiebere , Sofolahan Onyinye , Ugulu Rex Asibuodu , Ameyaw Ernest Effah 
Published: February 2024

Purpose
The purpose of this study is to assess the potential benefits of digital technologies (DTs) in bolstering the circular economy (CE) transition in the construction industry, to speed up the attainment of sustainable development objectives.

Design/methodology/approach
A detailed literature review was undertaken to identify DTs that could influence CE transition and the benefits of these DTs in the CE transitioning efforts of the construction industry. Based on these, a survey questionnaire was formulated and administered to construction professionals using convenient sampling techniques. With a response rate of 49.42% and data reliability of over 0.800, the gathered data were analysed using frequency and percentage, mean item score, normalisation value, coefficient of variation, Kendall’s coefficient of concordance, analysis of variance and factor analysis.

Findings
This study found that the construction experts agreed that building information modelling, blockchain technology, RFID, drone technology and cloud computing are the leading DTs that have the potential to influence and speed up CE transition in construction. Also, six clusters of benefits of DTs in bolstering EC are quicken CE transition, proactive waste management, recycling and zero waste, data management and decision-making, enhance productivity and performance and resource optimisation.

Originality/value
Studies on the integration of DTs in CE transition are scarce and it is even lacking in the Nigerian context. To the best of the authors’ knowledge, this study is the first to assess the role of DTs in CE transitioning in the Nigerian construction industry.
Intellectual capital efficiency: the missing piece to your corporate governance and profitability puzzle
Authors: Ijaz Misal , Zarrar Abeera , Naz Farah 
Published: February 2024

Purpose
The purpose of this study is to evaluate the synergy of corporate governance (CG) with intellectual capital (IC) and to assess the moderating effect of profitability indicator on the aforementioned synergy using agency theory, resource-based view theory and theory of financial ratios as conceptual frameworks.

Design/methodology/approach
The sample includes 72 companies with a six-year data set drawn from the KSE 100 Index companies of Pakistan. In addition, the study adopts Pulic’s model to compute the efficiency of IC. The research uses fixed-effect panel regression for analysis and two-stage least squares regression (2SLS) to address endogeneity issues in the estimation process.

Findings
The results showcased that chief executive officer duality possesses negligible impact on IC efficiency (ICE), while independent directors, audit committees and board size tend to attain a strong association with IC. Moreover, it postulates that the moderation of return on equity strengthens the path between all governance components and ICE significantly.

Originality/value
The research uses a 2SLS regression analysis to explore how CG practices take hold on the effectiveness of IC in Pakistan while taking into account the moderating impact of profitability. The findings add to the body of knowledge on the value that strong governance practices have on businesses and society.
Self-determination theory and customer revenge behavior: explaining how customers regulate their anger and revenge behavior
Authors: Shahrasbi Nasser , Rohani Mina , Purmehdi Mostafa , Rajabzadeh Ghatari Ali 
Published: February 2024

Purpose
This study aims to explore and empirically examine an integrative model of the customer revenge process by linking two well-established theories of self-determination theory (SDT) and appraisal theory.

Design/methodology/approach
A survey of 901 respondents, followed by a post-hoc survey of 712 individuals, was conducted to examine the autonomous versus controlled orientations for revenge motivation.

Findings
The results show that customers’ orientation of motivation (OM) can regulate their revenge behavior (direct versus indirect) in case of service failures. Specifically, the interaction of OM components (i.e. autonomy, relatedness and competence) can play a significant role in the relationship between revenge predictors and revenge behavior. Implications for research and practice are discussed.

Originality/value
This paper offers a novel conceptual framework to explain the moderating effects of OM on the relationship between revenge predictors and revenge behavior. This study extends the application of SDT to the context of customer anger and revenge.
Free banking theory: literature review and relevance to the regulation of cryptocurrencies debate
Authors: Norton Simon D. 
Published: February 2024

Purpose
Free banking theory, as developed in Adam Smith’s 1776 treatise, “The Wealth of Nations” is a useful tool in determining the extent to which the “invisible hand of the market” should prevail in regulatory policy. The purpose of this study is to provide a timely review of the literature, evaluating the theory’s relevance to regulation of financial technology generally and cryptocurrencies (cryptos) specifically.

Design/methodology/approach
The methodology is qualitative, applying free banking theory as developed in the literature to technology-defined environments. Recent legislative developments in the regulation of cryptocurrencies in the UK, European Union and the USA, are drawn upon.

Findings
Participants in volatile cryptocurrency markets should bear the consequences of inadvisable investments in accordance with free banking theory. The decentralised nature of cryptocurrencies and the exchanges on which these are traded militate against coordinated oversight by central banks, supporting a qualified free banking approach. Differences regarding statutory definitions of cryptos as units of exchange, tokens or investment securities and the propensity of these to transition between categories across the business cycle render attempts at concerted classification at the international level problematic. Prevention of criminality through extension of Suspicious Activity Reporting to exchanges and intermediaries should be the principal objective of policymakers, rather than definitions of evolving products that risk stifling technological innovation.

Originality/value
The study proposes that instead of a traditional regulatory approach to cryptos, which emphasises holders’ safety and compensation, a free banking approach combined with a focus on criminality would be a more effective and pragmatic way forward.
Electricity energy-saving behavior of households in Jordan: a qualitative study
Authors: Alwedyan Safaa 
Published: February 2024

PurposeGiven the rapid increase in energy consumption in the residential sector in Jordan recently, the question of how to promote energy-saving behavior in Jordanian households is an emerging topic that is receiving increasing attention from scholars and academics. Generally, there is an unresolved paradox in the literature concerning electricity-saving behaviors. On one hand, numerous studies highlight energy-saving behaviors. On the other hand, recent research indicates the presence of significant untapped potential in electricity-saving behaviors. Therefore, it is useful to revisit the construct of these behaviors qualitatively to expand understanding. The study aimed to provide a better understanding of electricity energy-saving behaviors in terms of its motivations, barriers and support mechanisms from household heads' or household members' perspectivesDesign/methodology/approachQualitative study in a sample of households in north Jordan was conducted in the Irbid province using grounded theory methodology. The analysis of qualitative data involved coding, followed by the integration of codes into more comprehensive categories and themes and interpreting the findings.FindingsThe results identify the motivations for households to save energy, the main barriers to indulging in electricity energy-saving behaviors, and the main support mechanisms and perceived support of electricity energy-saving behaviorsPractical implicationsThe findings bear significant implications for targeted interventions in the study area, improving motivations and addressing local barriers and can inform future policy issues by tailoring initiatives to the specific context.Originality/valueThis study is distinguished by being the first study that specializes in electricity energy-saving behavior of households in Jordan, using new methodology and techniques (qualitative survey).