bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive review of 3D cancer models for drug screening and translational research
Authors: Sharma Karthikey , Dey Sreenath , Karmakar Rounik , Rengan Aravind Kumar 
Published: December 2023

AbstractThe 3D cancer models fill the discovery gap of 2D cancer models and play an important role in cancer research. In addition to cancer cells, a range of other factors include the stroma, density and composition of extracellular matrix, cancer‐associated immune cells (e.g., cancer‐associated fibroblasts cancer cell‐stroma interactions and subsequent interactions, and a number of other factors (e.g., tumor vasculature and tumor‐like microenvironment in vivo) has been widely ignored in the 2D concept of culture. Despite this knowledge, the continued use of monolayer cell culture methods has led to the failure of a series of clinical trials. This review discusses the immense importance of tumor microenvironment (TME) recapitulation in cancer research, prioritizing the individual roles of TME elements in cancer histopathology. The TME provided by the 3D model fulfills the requirements of in vivo spatiotemporal arrangement, components, and is helpful in analyzing various different aspects of drug sensitivity in preclinical and clinical trials, some of which are discussed here. Furthermore, it discusses models for the co‐assembly of different TME elements in vitro and focuses on their synergistic function and responsiveness as tumors. Furthermore, this review broadly describes of a handful of recently developed 3D models whose main focus is limited to drug development and their screening and/or the impact of this approach in preclinical and translational research.
First evidence of AXL expression on circulating tumor cells in metastatic breast cancer patients: A proof‐of‐concept study
Authors: Bardol Thomas , Eslami‐S Zahra , Masmoudi Doryan , Alexandre Marie , Duboys de Labarre Marie , Bobrie Angélique , D'Hondt Véronique , Guiu Séverine , Kurma Keerthi , Cayrefourcq Laure , Jacot William , Alix‐Panabières Catherine 
Published: December 2023

AbstractBackgroundFor several years, the AXL tyrosine kinase receptor, a member of the Tyro3‐Axl‐Mer (TAM) family, has been considered a new strategic target in oncology. AXL overexpression is common in solid tumors and is associated with poor prognosis. In this context, the detection of a subset of circulating tumor cells (CTCs) that express AXL (AXL+ CTCs) could be clinically relevant.MethodsImmunostaining was performed to assess AXL expression in human breast cancer cell lines. The optimal conditions were established using flow cytometry. Spiking experiments were carried out to optimize the parameters of the CellSearch® system detection test. CTC enumeration and AXL expression were evaluated in patients with metastatic breast cancer (mBC) before treatment initiation.ResultsAn innovative AXL+ CTC detection assay to be used with the CellSearch® system was developed. In a prospective longitudinal clinical trial, blood samples from 60 patients with untreated mBC were analyzed to detect AXL+ CTCs with this new assay. CTCs were detected in 35/60 patients (58.3%) and AXL+ CTCs were identified in 7 of these 35 patients (11.7% of all patients).ConclusionThis newly established AXL+ CTC assay is a promising tool that can be used for liquid biopsy in future clinical trials to stratify and monitor patients with cancer receiving anti‐AXL therapies.
Immune cells within tertiary lymphoid structures are associated with progression‐free survival in patients with locoregional recurrent breast cancer
Authors: Gu Jinyuan , Wang Jiaming , Sun Yue , Mao Xinrui , Qian Chao , Tang Xinyu , Wang Ji , Xie Hui , Ling Lijun , Zhao Yi , Liu Xiaoan , Zhang Kai , Pan Hong , Wang Shui , Wang Cong , Zhou Wenbin 
Published: December 2023

AbstractIntroductionLocoregional recurrent breast cancers have a poor prognosis. Little is known about the prognostic impact of immune microenvironment, and tertiary lymphoid structures (TLSs) in particular have not been reported. Thus, we aimed to characterize the immune microenvironment in locoregional recurrent breast tumors and to investigate its relationship with prognosis.MethodsWe retrospectively included 112 patients with locoregional recurrent breast cancer, and hematoxylin–eosin staining and immunohistochemical staining (CD3, CD4, CD8, CD19, CD38, and CD68) were performed on locoregional recurrent tumor samples. The association of immune cells and TLSs with progression‐free survival (PFS) were analyzed by survival analysis.ResultsWe found more immune cells in the peritumor than stroma. After grouping according to estrogen receptor (ER) status, a low level of peritumoral CD3+ cells in ER+ subgroup (p = 0.015) and a low level of stromal CD68+ cells in ER− subgroup (p = 0.047) were both associated with longer PFS. TLSs were present in 68% of recurrent tumors, and CD68+ cells within TLSs were significantly associated with PFS as an independent prognostic factor (p = 0.035). TLSs and immune cells (CD3, CD38, and CD68) within TLSs were associated with longer PFS in ER− recurrent tumors (p = 0.044, p = 0.012, p = 0.050, p < 0.001, respectively), whereas CD38+ cells within TLSs were associated with shorter PFS in ER+ recurrent tumors (p = 0.037).ConclusionOur study proposes potential predictors for the clinical prognosis of patients with locoregional recurrent breast cancer, emphasizing the prognostic value of immune cells within TLSs, especially CD68+ cells.
Stability challenges and prospects for the industrial application of non‐noble catalysts for selective catalytic reduction of NOx by CO (CO‐SCR)
Authors: Li Shuhao , Wang Feng , Ng Derrick , Shen Boxiong , Xie Zongli 
Published: December 2023

AbstractThe selective catalytic reduction of NOx by CO (CO‐SCR) holds considerable promise as a simple and cost‐effective technology capable of concurrently removing CO and NOx from mobile and stationary sources. This is especially pertinent for industry sectors with high CO emissions, such as the iron and steel industry, making CO‐SCR an attractive solution with significant industrial applicability. However, despite the promising industrial potential of CO‐SCR technology, its widespread adoption is hindered by several factors, most notably the impacts of SO2, H2O, and O2 on catalyst performance and stability. Current research on these limiting factors is not exhaustive, posing challenges for the comprehensive industrial implementation of CO‐SCR technology. Therefore, this review paper focuses on the variables impeding the industrial utilization of CO‐SCR, summarizing existing bottlenecks and emphasizing the urgent need to address the inhibitory effects of O2 on the CO‐SCR reaction, a critical issue in contemporary research. This review aims to clearly articulate the key challenges of CO‐SCR technology and provide direction for future research.
Extending the solid solution range of sodium ferric pyrophosphate: Off‐stoichiometric Na3Fe2.5(P2O7)2 as a novel cathode for sodium‐ion batteries
Authors: Pu Xiangjun , Yang Kunran , Pan Zibing , Song Chunhua , Lai Yangyang , Li Renjie , Xu Zheng‐Long , Chen Zhongxue , Cao Yuliang 
Published: December 2023

AbstractIron‐based pyrophosphates are attractive cathodes for sodium‐ion batteries due to their large framework, cost‐effectiveness, and high energy density. However, the understanding of the crystal structure is scarce and only a limited candidates have been reported so far. In this work, we found for the first time that a continuous solid solution, Na4−αFe2+α/2(P2O7)2 (0 ≤ α ≤ 1, could be obtained by mutual substitution of cations at center‐symmetric Na3 and Na4 sites while keeping the crystal building blocks of anionic P2O7 unchanged. In particular, a novel off‐stoichiometric Na3Fe2.5(P2O7)2 is thus proposed, and its structure, energy storage mechanism, and electrochemical performance are extensively investigated to unveil the structure–function relationship. The as‐prepared off‐stoichiometric electrode delivers appealing performance with a reversible discharge capacity of 83 mAh g−1, a working voltage of 2.9 V (vs. Na+/Na), the retention of 89.2% of the initial capacity after 500 cycles, and enhanced rate capability of 51 mAh g−1 at a current density of 1600 mA g−1. This research shows that sodium ferric pyrophosphate could form extended solid solution composition and promising phase is concealed in the range of Na4−αFe2+α/2(P2O7)2, offering more chances for exploration of new cathode materials for the construction of high‐performance SIBs.
Characterization of the Ligand Properties of Donor‐stabilized Pnictogenyltrielanes
Authors: Szlosek Robert , Niefanger Amelie Sophie , Balázs Gábor , Seidl Michael , Timoshkin Alexey Y. , Scheer Manfred 
Published: December 2023

AbstractA general synthesis and the characterization of novel alkyl‐substituted NHC‐stabilized pnictogenylboranes NHC ⋅ BH2ER2 (NHC=N‐heterocyclic carbene, E=P, As; R2=Me2, Ph2, tBuH, Cy2, (SiMe3)2) are reported. These compounds were reacted with Ni(CO)4 to the corresponding complexes of the type [(NHC ⋅ BH2ER2)Ni(CO)3] to determine their donor strength by Tolman Electronic Parameters (TEPs) and their steric demand as ligands compared to classical phosphines, superbasic phosphines and other commonly applied donor systems. The results show that the NHC‐stabilized pnictogenyltrielanes can be considered as being highly basic, while their steric influence depends strongly on the organic residues as well as the donor attached to the {BH2} moiety. Although weaker than commonly used superbasic phosphines, the donor strength of pnictogenyltrielanes in general can be classified as of similar strength as NHCs. The steric and electronic properties can easily be modified by alkyl substitution as evident from the TEP trends.
“Buttressing Effect” in the Halogen‐Lithium Exchange in ortho‐Bromo‐N,N‐dimethylanilines and Related Naphthalenes
Authors: Meshalkin Stepan A. , Tsybulin Semyon V. , Bardakov Victor G. , Tatarinov Ilya A. , Shitov Daniil A. , Tupikina Elena Y. , Efremova Mariia M. , Antonov Alexander S. 
Published: December 2023

AbstractNon‐covalent interactions such as coordination of an organolithium reagent by a directing group and steric repulsion of substituents strongly affect the halogen‐lithium exchange process. Here we present the manifestation of the “buttressing effect” – an indirect interaction between two substituents issued by the presence of a third group – and its influence on the ease and selectivity of the bromine‐lithium exchange and the reactivity of formed aryllithiums. The increase of the size of the “buttressing” substituent strongly affects the conformation of a NMe2 group, forcing it to hinder ortho‐bromine and thus slowing down the exchange. In naphthalene substrates bearing two bromines, this suppresses regioselectivity of the reaction. The “buttressing effect” forces formed aryllithiums to deaggregate, thus boosting their reactivity. This facilitates the decomposition via protolisys by ethereal solvents even at low temperatures and in some cases initiates fast Wurtz‐Fittig coupling.
Research on the working mode, working state, and control strategies of generalized electric spring
Authors: Wang Xiaohu , Chen Xinyuan , Huang Zhun , Zhao Chaohui 
Published: December 2023

SummaryThe advantage of a generalized electric spring (G‐ES) is that it solves the redundancy problem of a conventional electric spring (ES) in the face of critical load clusters. This paper first introduces Hamilton's principle into the G‐ES, derives the mathematical model of the G‐ES, and further introduces the topology of the G‐ES. Next, the working modes and working states of the G‐ES are defined. The working modes are used to reflect the combination of ES power inside the G‐ES, and the power conditions in specific working modes are described using a phase diagram. Then, the paper analyses the power case of the G‐ES to the external system, giving the respective working states according to their internal working modes. Finally, this paper introduces PQ control to the control strategy of the G‐ES and achieves control of the G‐ES working mode and its working state. The results of the study show that there are 100 working modes of the G‐ES, and 76 working modes have a specific working state. The correctness of the working mode and working state analysis is verified with the help of the MATLAB/Simulink simulation and experiment.
Molecular analysis of XPO1 inhibitor and gemcitabine–nab‐paclitaxel combination in KPC pancreatic cancer mouse model
Authors: Uddin Md. Hafiz , Al‐Hallak Mohammad Najeeb , Khan Husain Yar , Aboukameel Amro , Li Yiwei , Bannoura Sahar F. , Dyson Gregory , Kim Seongho , Mzannar Yosef , Azar Ibrahim , Odisho Tanya , Mohamed Amr , Landesman Yosef , Kim Steve , Beydoun Rafic , Mohammad Ramzi M. , Philip Philip A. , Shields Anthony F. , Azmi Asfar S. 
Published: December 2023

AbstractBackgroundThe majority of pancreatic ductal adenocarcinoma (PDAC) patients experience disease progression while on treatment with gemcitabine and nanoparticle albumin‐bound (nab)‐paclitaxel (GemPac) necessitating the need for a more effective treatment strategy for this refractory disease. Previously, we have demonstrated that nuclear exporter protein exportin 1 (XPO1) is a valid therapeutic target in PDAC, and the selective inhibitor of nuclear export selinexor (Sel) synergistically enhances the efficacy of GemPac in pancreatic cancer cells, spheroids and patient‐derived tumours, and had promising activity in a phase I study.MethodsHere, we investigated the impact of selinexor–gemcitabine–nab‐paclitaxel (Sel‐GemPac) combination on LSL‐KrasG12D/+; LSL‐Trp53R172H/+; Pdx1‐Cre (KPC) mouse model utilising digital spatial profiling (DSP) and single nuclear RNA sequencing (snRNAseq).ResultsSel‐GemPac synergistically inhibited the growth of the KPC tumour‐derived cell line. The Sel‐GemPac combination reduced the 2D colony formation and 3D spheroid formation. In the KPC mouse model, at a sub‐maximum tolerated dose (sub‐MTD) , Sel‐GemPac enhanced the survival of treated mice compared to controls (p < .05). Immunohistochemical analysis of residual KPC tumours showed re‐organisation of tumour stromal architecture, suppression of proliferation and nuclear retention of tumour suppressors, such as Forkhead Box O3a (FOXO3a). DSP revealed the downregulation of tumour promoting genes such as chitinase‐like protein 3 (CHIL3/CHI3L3/YM1) and multiple pathways including phosphatidylinositol 3'‐kinase‐Akt (PI3K‐AKT) signalling. The snRNAseq demonstrated a significant loss of cellular clusters in the Sel‐GemPac‐treated mice tumours including the CD44+ stem cell population.ConclusionTaken together, these results demonstrate that the Sel‐GemPac treatment caused broad perturbation of PDAC‐supporting signalling networks in the KPC mouse model.Highlights
The majority of pancreatic ductal adenocarcinoma (PDAC) patients experience disease progression while on treatment with gemcitabine and nanoparticle albumin‐bound (nab)‐paclitaxel (GemPac).
Exporter protein exportin 1 (XPO1) inhibitor selinexor (Sel) with GemPac synergistically inhibited the growth of LSL‐KrasG12D/+; LSL‐Trp53R172H/+; Pdx1‐Cre (KPC) mouse derived cell line and enhanced the survival of mice.
Digital spatial profiling shows that Sel‐GemPac causes broad perturbation of PDAC‐supporting signalling in the KPC model.
Beetle colony optimization algorithm‐based node clustering scheme for efficient data dissemination in vehicular ad hoc networks
Authors: Nithyanandam Gopinath , Ambayiram Chinnasamy , Natarajan Bhalaji 
Published: December 2023

SummaryVehicular ad hoc networks (VANETs) are the ultimate solution for preventing road accidents, which result in the loss of precious human life worldwide. In this context, effective communication between the vehicular nodes is essential due to the varying network topology and high vehicular mobility inherent with VANETs. Cluster‐based routing is identified to be a significant approach for achieving efficient routing and improving communication proficiency in VANETs. In this paper, a beetle colony optimization algorithm–based clustering scheme (BCOACS) is proposed for generating optimized clusters for facilitating reliable data dissemination. This BCOACS algorithm includes two vital strategies such as beetle antenna search (BAS) and swarm intelligence for attaining inter‐cluster and intra‐cluster communications. In specific, BAS strategy that includes random search attributed toward gradient direction is used for intra‐cluster communication without using the complete amount of gradient information. On the other hand, a swarm intelligence strategy that encompasses a collective approach of self‐organized and decentralized agents is used for inter‐cluster communication with the view to minimize the load on each cluster head (CH) and to extend the clusters' lifetime. The simulation outcomes of the proposed BCOACS scheme confirmed improved performance in optimizing the number of constructed clusters independent of the increase in the network grid size, transmission range, and number of vehicular nodes in the network compared to the benchmarked approaches. The results also confirmed that the proposed BCOACS scheme achieved a maximized throughput of 13.42%, with reduced delay and protocol overhead of 18.96% and 19.45%, better than the benchmarked schemes used for investigation.
Plasma p‐tau217, p‐tau181, and NfL as early indicators of dementia risk in a community cohort: The Shanghai Aging Study
Authors: Xiao Zhenxu , Wu Wanqing , Ma Xiaoxi , Wu Jie , Liang Xiaoniu , Zhou Xiaowen , Cao Yang , Zhao Qianhua , Ding Ding 
Published: December 2023

AbstractINTRODUCTIONBlood biomarkers showed values for predicting future cognitive impairment. Evidence from the community‐based cohort was limited only in high‐income countries.METHODSThis study included 1857 dementia‐free community residents recruited in 2009–2011 and followed up in waves 2014–2016 and 2019–2023 in the Shanghai Aging Study. We intended to explore the relationships of baseline plasma ALZpath phosphorylated tau 217 (p‐tau217), p‐tau181, neurofilament light chain (NfL) with follow‐up incident dementia, Alzheimer's disease (AD), and amyloidosis.RESULTSHigher concentrations of plasma p‐tau217, p‐tau181, and NfL were correlated to higher decline speed of Mini‐Mental State Examination score, and higher risk of incident dementia and AD. The p‐tau217 demonstrated a significant correlation with longitudinal neocortical amyloid‐beta (Aβ) deposition (r = 0.57 [0.30, 0.76]) and a high accuracy differentiating Aβ+ from Aβ‐ at follow‐ups (area under the receiver operating characteristic curve = 0.821 [0.703, 0.940]).DISCUSSIONPlasma p‐tau217 may be an early predictive marker of AD and Aβ pathology in older community‐dwelling individuals.Highlights
Plasma p‐tau217, p‐tau181, and NfL were positively associated with long‐term cognitive decline and risk of incident dementia.
Plasma p‐tau217 showed a better performance distinguishing Aβ+ individuals from Aβ‐ individuals at follow‐ups.
Plasma NfL may be a suitable predictor of general cognitive decline in older community‐dwelling individuals.
A preliminary site selection system for underground hydrogen storage in salt caverns and its application in Pingdingshan, China
Authors: Huang Liangchao , Fang Yanli , Hou Zhengmeng , Xie Yachen , Wu Lin , Luo Jiashun , Wang Qichen , Guo Yilin , Sun Wei 
Published: December 2023

AbstractLarge‐scale underground hydrogen storage (UHS) provides a promising method for increasing the role of hydrogen in the process of carbon neutrality and energy transition. Of all the existing storage deposits, salt caverns are recognized as ideal sites for pure hydrogen storage. Evaluation and optimization of site selection for hydrogen storage facilities in salt caverns have become significant issues. In this article, the software CiteSpace is used to analyze and filter hot topics in published research. Based on a detailed classification and analysis, a “four‐factor” model for the site selection of salt cavern hydrogen storage is proposed, encompassing the dynamic demands of hydrogen energy, geological, hydrological, and ground factors of salt mines. Subsequently, 20 basic indicators for comprehensive suitability grading of the target site were screened using the analytic hierarchy process and expert survey methods were adopted, which provided a preliminary site selection system for salt cavern hydrogen storage. Ultimately, the developed system was applied for the evaluation of salt cavern hydrogen storage sites in the salt mines of Pingdingshan City, Henan Province, thereby confirming its rationality and effectiveness. This research provides a feasible method and theoretical basis for the site selection of UHS in salt caverns in China.
Organised loneliness and its discontents
Authors: Sagan Olivia 
Published: December 2023

AbstractThis review paper offers a critique of the discourse of loneliness both in the popular and academic imagination. It questions the stance and approach of much loneliness research and the headlines that have been extracted from it. These position loneliness as an epidemic, framing it as a global public health problem, its aetiology and management located in the individual. The paper draws attention to overlooked alternative framings of loneliness as well as to the risks of maintaining our current levels of alarm regarding it. Finally, the work of Hannah Arendt is turned to, as part of a wider academic reappreciation of her work on loneliness. The paper ends by suggesting what can be learned by loneliness researchers in the medical humanities from such political analyses.
What medical providers need to elevate outpatient care for adolescents and young adults with binge‐eating disorder: A novel protocol
Authors: Downey Amanda E. , Odette Michelle , Sanders Austin E. , Kuykendall Mikayla , Saunders Elizabeth , Nagata Jason M. , Forsberg Sarah , Buckelew Sara M. , Garber Andrea K. 
Published: December 2023

AbstractPurposeBinge‐eating disorder (BED) in adolescents and young adults is underrecognized and understudied and no standardized guidelines exist for medical providers caring for this population. To highlight the lack of extant evidence, we examine the demographic characteristics of youth with BED in an academic eating disorders (EDs) program and primary care clinic and describe the needs of their medical care providers.MethodA retrospective chart review was conducted for patients who met criteria for BED from July 2021 to June 2022. We surveyed their medical providers to understand their needs in caring for this population. A multidisciplinary team with expertise in the care of youth with EDs amalgamated current evidence in caring for youth with BED into a protocolized care schema designed for implementation in the outpatient medical setting.ResultsEighteen youth with BED were reviewed, 14 identified as female, 3 as male, and 1 as “Other.” Average age was 15.4 (2.7) years old, and mean body mass index was 35.90 (8.25). 33.3% (6) patients identified as White/Caucasian, followed by 22.2% (4) Hispanic/Latinx. Eleven of 18 were privately insured. The most common medical recommendations were for regular meals and snacks and for individual psychotherapy. Medical providers desired more education about identification and management of youth with BED.ConclusionsTo address the lack of medical care guidelines for youth with BED, recommendations in this Forum include: increased education for medical providers, standardized medical monitoring, an emphasis on psychotherapy, and a weight‐inclusive orientation.Public SignificanceAdolescents and young adults with BED are underrecognized and undertreated. Little is known about the characteristics of these patients and the medical care these patients receive within academic EDs program. For the first time, preliminary recommendations for medical care are provided.