bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological, immunological and nutritional assessment of Penaeus vannamei fed with different combinations of novel feed ingredients
Authors: Rajalakshmi Kalaivanan , Felix Nathan , Ranjan Amit , Arumugam Uma , Sathishkumar Govindharaj 
Published: January 2025

Abstract
An eight-week feeding trial was carried out to examine the effects of diets formulated with different combinations of novel feed ingredients as substitutes for fishmeal (FM). The research focused on evaluating their influence on fatty acid and amino acid profiles, immunological and plasma biochemical analysis, immune related gene expression and histological analysis of Pacific white shrimp, Penaeus vannamei. Five diets were developed for Penaeus vannamei, all of which were isonitrogenous (36%) and isolipidic (6%). Diet-1 (Control): primary source of protein was fish meal (FM); Diet 2: replaced FM with a 1:1 ratio of poultry by-product meal (PBM) and single cell protein (SCP); Diet 3: replaced FM with a 1:1:1 ratio of insect meal (IM), rapeseed meal (RM), and SCP; Diet-4 replaced FM with fish waste (FW), peanut meal (PM), and SCP in a 1:1:1 ratio; Diet-5 replaced FM with PBM, SCP, IM, FW, PM, and RM in equal amounts (1:1:1:1:1:1). Nursery reared juvenile shrimps (1.05± 0.03 g) were stocked in triplicates in the experimental tanks at the rate of 35 nos. shrimp per tank. The plasma biochemical responses and amino acid profile did not differ significantly (p>0.05) between the dietary groups. EPA was significantly higher in diet 1 and diet 5 which was not different from diet 4 and DHA was significantly higher in diet 1 which was not different from diet 5. Prophenoloxidase activity (proPO) was significantly higher (p<0.05) in diet 1 and diet 5, and Respiratory burst activity was significantly (p<0.05) higher in diet 1 and diet 5 which was not different from diet 3. The immune gene expression (LYZ) was upregulated in diet 1 and diet 5. More B cells and few number of R cells were observed in diet 1 and diet 5 compared to other diets. Thus, the present study concluded that a mixture of PBM, SCP, IM, RM, PM and FW (1:1:1:1:1:1) serve as a good combination diet to totally replace FM in shrimp feed without compromising the fatty acid and amino acid profile and health of Pacific white shrimp (P. vannamei).
Effects of dietary algae meal supplementation on growth, whole-body composition, histological and immune responses of Pacific white shrimp, Penaeus vannamei
Authors: Prabu Elangovan , Felix Nathan , Sivasankar Sundaram , Thirumalai Arangannal , Uma Arumugam , Manikandan Kalidoss , Sathishkumar Govindharaj , Thiruvasagam Thangaraju 
Published: January 2025

Abstract
Algae meal is an emerging shrimp feed additive due to its essential nutrient composition, such as fatty acids, amino acids, vitamins, and minerals. A 60-day feeding trial examined the effects of algae meal as an additive on growth, whole-body composition, and histological and immune responses of Pacific white shrimp, Penaeus vannamei. Six iso-protien and iso-lipid treatment diets were formulated and prepared using best mix feed formulation software and pelletizer, respectively. The diet without supplementation of algae meal was used as a control. Two treatment diets included algae meal at 0.5% (T1-AM) and 1% (T2-AM). After pelletization, another three treatment diets were vacuum coated with algae meal at 0.5% (T3-AM), 1% (T4-AM), and a combination of 0.5% algae meal and 0.5% probiotic (T5-AMP). A total of 900 juvenile shrimp, with an initial average weight of 3.12 ± 0.07g, were evenly distributed into 18 rectangular cages in three indoor cement tanks (5.0 m × 3.0 m × 1.2 m). Each diet was randomly allotted to triplicate groups of 50 shrimps (average weight of 3.12 ± 0.07g) per treatment and fed thrice daily (9:00, 13:00, and 17:00 H). The results showed that significantly higher water stability of feed, water absorption (%), and dry matter retention were observed in the T5-AMP diet (P<0.05). Similarly, higher weight gain, protein efficiency ratio, specific growth rate, and better feed conversion ratio were observed in the shrimp-fed T5-AMP diet (P<0.05). The whole-body proximate and amino acid composition of shrimp were not affected by the inclusion and coating of algae meal and probiotic diets (P>0.05). Moreover, no significant abnormalities were found in the histology analysis of the hepatopancreas and intestine of shrimp-fed experimental diets. The relative mRNA expression of immune-related genes was significantly upregulated in shrimp fed a T5-AMP diet (P<0.05). In conclusion, this study indicated that 0.5% algae meal blended with probiotics through vacuum coating could improve the feed quality, nutrient utilization, growth performance, and immune responses of P. vannamei. A blend of algae meal and probiotics could help to develop nutritious immune-boosting shrimp feed and ensure sustainable shrimp farming practices.
Non-antibiotic growth promoters in poultry nutrition
Authors: Ishaq Hafiz Muhammad , Ishaq Waqas , Usman Muhammad , Ghayas Abdul , Yameen Rao Muhammad Kashif , Abdullah  , Saleem Kinza , Ahmad Sohail 
Published: January 2025

Abstract
Poultry meat and eggs are considered as major sources of food for rapidly increasing human population across the globe. During the rearing of poultry, different antibiotics were included in poultry diets in sub therapeutic doses to attain improvements in growth performance. Recently, the use of antibiotics in poultry production has been considered as one of the major reasons behind the emergence of antibiotic resistance in human and poultry pathogens. The pressing issue of antibiotic resistance led to complete or partial bans on the use of antibiotics as growth promoters in many parts of the world. Moreover, there have been increased concerns among the consumers about the antibiotic residues in poultry products. These administrative bans led to an increased incidence of bacterial disease outbreaks thus compromising the poultry performance, welfare, and economic outcomes of poultry producers. This ultimately attracted the attention of researchers to find the alternative strategies that could replace the antibiotics and bring production, health, and food safety benefits to the poultry production systems. These non-antibiotic growth promoters mainly include probiotics, prebiotics, synbiotics, phytogenic substances, organic acids, antimicrobial peptides, enzymes, bacteriophages, and egg yolk antibodies. Inclusion of these non-antibiotic solutions in poultry diets demonstrates promising results in terms of production performance and birds’ health. These promising results are demonstrated through improvements in nutrient absorption, proliferation of beneficial bacteria, reduction in pathogenic bacterial species, production of bacterial metabolites that serve as energy sources for intestinal epithelial cells of the host, and positive modulation of immune responses. Apart from reducing the colonization of bacterial species that are pathogenic for poultry, these alternative solutions have also exhibited satisfactory efficacy in reducing the colonization of foodborne pathogens like Salmonella and Campylobacter jejuni which cause illness in the human population. In this paper, we reviewed studies that evaluated the effects of non-antibiotic growth promoters on different types of poultry. A description of mechanism of action, advantages, disadvantages and effects on production performance, gut health and immune parameters are discussed in this paper.
Identification of genes related to fat deposition as candidates for er stress based on combined RNA-ATAC sequencing analysis
Authors: Piórkowska Katarzyna , Żukowski Kacper , Witarski Wojciech , Kowalska Katarzyna , Ropka-Molik Katarzyna , Tyra Mirosław 
Published: January 2025

Abstract
Endoplasmic reticulum (ER) stress is associated with obesity as this state challenges lipid and carbohydrate metabolisms, resulting in glucolipotoxicity and endoplasmic reticulum dysfunction. It can be induced by excessive ectopic fat accumulation, rising blood glucose levels or meta-inflammatory factors, which disturb the liver’s numerous pathways favoring hepatic lipogenesis. In the present study, It was attempted to identify liver molecular processes associated with fat deposition in pigs based on combined RNA-ATAC-seq analysis. The pig groups used in the present study were significantly different in terms of subcutaneous and visceral fat deposition; they belonged to a native Polish breed and were not under selection pressure. Based on RNAATAC-seq combined analysis, it was identified 45 significant differentially expressed genes (DEGs) in liver tissue dependent on fat deposition, for which open chromatin regions in transcription start sites were found. The functional analysis pinpointed that 5 of them are involved in the ER stress process (MANF, SELENOS, HYOU1, PIK3R1, and HSPA5). These five proposed genes expressed in the liver as candidates associated with fat deposition in pigs because ER stress plays a significant role in this organ in molecular process associated with the determination of fat level in the organism, which was previously broadly described in humans.
Technical and Economic Analysis of a Robotic Station for Assembling the FPV Drone Body
Authors: Górski Marcin , Prykhodzhenko Valerii , Karamon Tomasz , Caban Jacek 
Published: January 2025

Abstract
The current technological progress is possible due to automated assembly and transport operations, and the increasing use of robotic cells. This article presents a robotic station for assembling the body of an FPV drone. The article uses analyzes and simulations as well as the results of simulation studies. The research has shown that the robotic station significantly increases the efficiency of the assembly process compared to traditional methods, shortening the unit production time by approximately 30-50%. The economic analysis has shown that despite higher initial costs, the investment in a robotic station pays off in approximately 3.3 years, which results from lower operating costs and the elimination of some labor costs. Moreover, the automation of assembly processes not only contributes to financial savings, but also to the improved quality of the final product. The future research should focus on further optimization of robotic stations, in particular on increasing their flexibility and adaptability in dynamic production environments.
Views of the Baltic German Press on the Traditions: of Other Nationalities in Riga’s Music Life in the 1870s–1880s: The Influence of the Political Context
Authors: Jaunslaviete Baiba 
Published: January 2025

Abstract
The history of German presence in the territory of Latvia began in the thirteenth century, when Germans arrived here as crusaders. From that time until the nineteenth century, they played the dominant role in the economic and political life of the region. In the second half of the nineteenth century, however, the situation changed. After the abolition of serfdom (1817–1819), Latvians greatly improved their economic and educational status, and the new Latvian intelligentsia expressed its opposition to German dominance in the region. The Latvian national revival of that period was supported by the Russian authorities with a somewhat selfish interest.
The aim of this paper is to answer the question: how did these processes affect the descriptions of music life in the press, or, more precisely, one aspect of press commentaries – the Baltic Germans’ views on the traditions of other nationalities?
It has been found that many reviews confirm the Baltic Germans’ interest and openness towards other national traditions. At least some of the German music critics do not deserve to be criticised for ignoring the music life of ethnic Latvians as described in Latvian periodicals. The political context, however, comes to the fore when discussing not the music itself, but the press publications or speeches that include some ‘provocative’ elements – be it claims to the premises of the Rigaer Stadttheater voiced in a Russian newspaper, or efforts to deny the inspiring role of Germans in Latvian cultural history.
The use of sonar data for mapping the muddy bottom of water reservoir
Authors: Pastusiak Tadeusz 
Published: January 2025

Abstract
The goal of the work was to find a method to determine the structure of the subsurface of the bottom of a water reservoir with relatively soft ooze-muddy sediments using the recorded parameters of the first and second sonar echoes. This would allow avoiding the use of expensive devices such as sub-bottom profilers.
Sonar measurements were taken on the entire lake commencing from the depth accessible to the measuring boat with outboard transducers. The sonar screen image and the first and second echo parameters were recorded and used according to the RoxAnn method. In order to verify and extend the scope of the sonar data, measurements of the bottom depth and hardness were made using a pole at 40 control points located along five cross sections.
Finally achieved numerical models of sediment structures under the bottom surface of the water reservoir for three reference surfaces: depths for initial top surface of the colloidal state (ooze) layer, top surface of dense muddy bottom and surface of solid bottom under muddy layer. Each of them was obtained by two independent methods, in which the input data was the depth measured by the sonar for the initial top surface of the colloidal state layer or roughness of the first echo.
The volume between the numerical surfaces for the colloidal state layer, which is important for environmental protection purposes, was calculated using three different methods. The results were very similar. The difference of volume was within 1.7–5.7%. Each of the method of measuring any layer volume using a single beam echo sounder / sonar can be used to assess the scope of work and the costs of required restoration measures or water reservoir by removal of colloid suspension layer. The advantage of the developed method is the possibility of performing the work using a single beam echo sounder / sonar instead of an expensive sub-bottom profiler.
A Location Effect on the Capital Gains of Condominium Investment
Authors: Teeramungcalanon Monthinee , Rattanaprichavej Niti 
Published: January 2025

Abstract
This research focuses on the effect of locational factors on condominium capital gains investment in Thailand. It employed secondary data selected through judgmental sampling. Capital gains data spanning 5 to 15 years were collected quarterly from 1,381 condominium projects in Thailand. ANOVA and Pearson correlation were conducted to study the relationship between locations, capital gains, and price per square meter.
The main findings can be summarized in three aspects: capital gains rates, locations, and timing, indicating that investing in a condominium based solely on capital gains without considering rental fees may not be a favorable strategy. Furthermore, regarding locations and timing, no significant returns were contributed. Although each location does not significantly contribute to different capital gains, the research highlights that residential and commercial activities are the most critical factors for generating higher-than-average capital gains. As a result, residential areas, the Bangkok Central Business District (CBD), Bangkok suburbs, commercial districts, and high-density residential areas are the top five potential locations for investment. Moreover, the relationship between the capital gains and the prices per square meter exhibits three directions: positive, negative, and no relationship, depending on locations.
The Model of Real Estate Valuation as an Investor-Oriented Model
Authors: Kucharska-Stasiak Ewa 
Published: January 2025

Abstract
In the article, the role of demand and supply in the theory of value and valuation is discussed using the case of the real estate market. The research hypothesis tested is that demand is dominant in the valuation process. The purpose of the article is to present evidence in support of the research hypothesis and to explain why real estate valuations need the investor-oriented model of valuation. A critical analysis of the pertinent literature and legislation and the observation method were employed to this end. The need to use the investor-oriented model for real estate valuation is discussed based on: (1) the residual (development) method; (2) the valuation of properties’ potential; and (3) the valuation of a subdivided property to calculate an adjacency fee. The matter under consideration is important not only for theoretical deliberations but also for the practice of real estate valuation and management.
Subject Pedagogy Research Methodologies: Analysis of Academic Journal Articles Published by Affiliates of Japan Consortium of Subject Pedagogy Associations
Authors: Kishi Noriko , Yamamoto Takaharu 
Published: January 2025

Abstract
This study aimed to clarify issues in subject pedagogy research by examining changes in research methods over time, with a focus on articles published in academic association journals. To identify the research methods used, we extracted data from articles published by academic associations within the Consortium for Subject Pedagogy. Using a common subject framework to classify research methods, we examined the methodologies through data analysis and a literature review. The results showed that (1) research methods varied by subject, and classifications that were valid in one subject society were not optimal for others; (2) theoretical-philosophical research has been common in mathematics but less so in social studies, geography, and home economics; (3) practical-development and empirical-experiential research have been common across subjects; (4) critical inquiry is an important step beyond traditional approaches; (5) individualistic and traditional-technical approaches are important in subject pedagogy, and recognition of their significance remains a future challenge.
Ne Health: Bisphenol a and Early Puberty, a Systematic Literature Review
Authors: Pereira Natália Ferrini Laviola , Kavalek Débora Schmitt , Zanardo Larissa Goulart , Guerra Thais Cardoso , Lima Matheus Ramalho 
Published: January 2025

Objective: Investigate the relationship between BPA exposure and precocious puberty.

Theoretical Framework: The theoretical framework includes studies that aim to demonstrate human contamination by BPA; highlight its toxic potential to human health and the environment; and associate BPA exposure with alterations in pubertal development (Valadares and Pfeilsticker, 2012; Castro-Correia and Fontoura, 2015; Sonnenschein and Soto, 2010, among others).

Method: The methodology adopted for this research comprises a systematic literature review, analyzing experimental, epidemiological, and clinical studies available in databases such as PubMed, Scopus, and Web of Science.

Results and Discussion: The results revealed a positive correlation between BPA exposure and alterations in pubertal development, particularly in girls, with evidence pointing to the influence of this compound in accelerating sexual maturation. However, the methodological heterogeneity of the studies and the lack of consensus on the exact biological mechanisms indicate the need for more robust research.

Research Implications: This study contributes to the literature by demonstrating that BPA exposure may be a risk factor for precocious puberty, highlighting the importance of stricter regulatory policies and additional investigations to confirm and clarify this association.

Originality/Value: This study significantly contributes to the literature by gathering and synthesizing available evidence on the relationship between bisphenol A (BPA) exposure and precocious puberty. It provides a comprehensive overview of the experimental, epidemiological, and clinical data, highlighting the potential of BPA as an environmental risk factor for alterations in pubertal development, particularly in girls.Furthermore, the article identifies critical gaps in scientific knowledge, such as the lack of consensus on the exact biological mechanisms through which BPA accelerates sexual maturation and the methodological heterogeneity present in the analyzed studies. This critical approach reinforces the need for future research that is more robust and methodologically consistent. Finally, by emphasizing the importance of stricter regulatory policies to minimize BPA exposure, the study also contributes to a public health agenda, pointing to pathways for interventions that can mitigate the impacts of this endocrine disruptor on human health and the environment.
Characterization of Wastewater in The University Campus: A Case Study in Universitas Andalas, Indonesia
Authors: Ruslinda Yenni , Nur Ansiha , Komala Puti Sri , Leni Leni , Regia Rinda Andhita , Mardatillah Regina 
Published: January 2025

This study aims to analyze wastewater characteristics at Universitas Andalas Campus Limau Manis Padang encompassing physical, chemical, and biological attributes, while comparing them to established quality standards and estimating mass loading. Wastewater samples were collected from various sources, including domestic and specific wastewater. Sampling locations and times were chosen based on peak activity. Physical analysis revealed temperature ranging from 27.32 to 31.5Â°C and TSS concentrations from 130.00 to 580.00 mg/L. Chemical parameters displayed significant variation, with pH values between 2.27 and 9.70, BOD5Â concentrations spanning 108.46 to 506.59 mg/L, COD levels from 162.70 to 1,428.00 mg/L, and oil and grease content from 0.20 to 84.31 mg/L. N-total ranged from 47.07 to 309.16 mg/L, sulfate from 72.70 to 1,480.00 mg/L, and phosphate from 1.14 to 98.15 mg/L. Biological attributes showed total coliform counts exceeding 10,000 colonies per 1,000 mL, all surpassing quality standards. Notably, the BOD5/COD ratio indicated low biodegradable quality (values <0.5) for certain wastewater sources. Greywater emerged as the primary pollutant, with substantial mass loadings. Eventually, this study underscores the vital need for wastewater treatment before environmental discharge.Keywords:Â Characteristics, Domestic Wastewater, Campus, Quality Standards, Hazardous WastewateÂ