bluuebunny/crossref_metadata_2025_split huggingface dataset:
Household satisfaction with health services and choice of response strategies to malaria occurrence: The case of mountain communities of Elgon in Uganda
Authors: Siya Aggrey , Egeru Anthony , Kalule John B. , Lukwa Akim Tafadzwa , Mutai Noah , Hartnack Sonja 
Published: October 2023

Abstract
Background
In rural Africa, access to health services is often limited by factors ranging from economics to culture. Additionally, climate change is noted to severely undermine the fragile health systems of such regions. Consequently, climate sensitive diseases including malaria will severely impact human livelihoods and wellbeing. Measuring satisfaction with health service delivery in such areas provides an opportunity to improve the resilience of health systems to such threats. Additionally, understanding factors associated with the choice of response strategies to malaria especially at household level provides an opportunity to design context specific interventions. .
Methods
In this study, we used polytomous latent class analyses to group the participants ‘responses and an additive Bayesian modelling networks to explore satisfaction with health service delivery as well as factors associated with response strategies of households to malaria. We did this with focus on the rural parts of Uganda in Mount Elgon region. A cross sectional study design was employed including use of a semi-structured questionnaire with sections including; perceptions on climate change, perceptions on malaria, coping with malaria and perceptions regarding health service delivery. In total, 200 responds were recruited into the study.
Results
We found that approaches to malaria control and management at household level include; use of traditional (54.5%), private (20.5%) and publicly available services (25%). Regarding satisfaction with health services, 66.6% of the respondents were satisfied with health service components of information flow, drug/vaccine access and accessibility. Type of housing, livelihood sources, previous malaria experience and health services were strongly associated with responses to malaria occurrence at household level. The rest of the factors were weakly associated with malaria responses at household level.
Conclusion
The indigenous interventions utilized by households to manage and control malaria were largely dependent on their satisfaction with health service delivery components. Interventions thus ought to leverage on the local existing knowledge to optimize outcomes and ensure sustainable health. Future studies should focus on larger areas of Elgon and other mountainous areas so as to allow for comparable results.
Can Performance-Based Budgeting Improve Corporate Innovation? Evidence from Chinese Listed Firms
Authors: wang xueping , wu yuanyuan 
Published: October 2023

Abstract
This paper explores the correlation between performance-based budgeting and corporate innovation in China, utilizing a quasi-natural experiment. The study reveals a significant rise in patents among firms headquartered in provinces that have implemented performance-based budgeting compared to those that have not. The increase is particularly pronounced for firms operating in provinces facing higher financial pressure and stronger government audits. The positive impact of performance-based budgeting on corporate innovation is particularly noteworthy in state-owned firms, firms exhibiting good corporate governance practices, or firms facing high financial constraints. Collectively, the evidence implies that performance-based budgeting can serve as a catalyst for corporate innovation.
JEL: H61; M21; M41
Cross-kingdom RNAi adds a new layer of communication to the arbuscular mycorrhizal symbiosis
Authors: Lanfranco Luisa , Silvestri Alessandro , Ledford William , Fiorilli Valentina , Votta Cristina , Scerna Alessia , Tucconi Jacopo , Mocchetti Antonio , Grasso Gianluca , Balestrini Raffaella , Jin Hailing , Rubio-Somoza Ignacio 
Published: October 2023

Abstract
Cross-kingdom RNA interference (ckRNAi) is a mechanism of interspecies communication where small RNAs (sRNAs) are transported from one organism to another, where they silence target genes in trans1-9. ckRNAi is well established in pathosystems1-9 and has also been observed in a small number of mutualistic interactions10,11. Fungal and oomycete pathogens, as well as symbiotic bacterium, send sRNAs into host cells to hijack host RNAi machinery by loading into host AGO proteins in order to silence host target genes. Previous research from our group has suggested the possibility of ckRNAi occurring in Arbuscular Mycorrhizal Symbiosis (AMS)12, the most widespread and ancient symbiosis on Earth13. Here, we show that a specific sRNA from the AM fungus Rhizophagus irregularis (Rir2216) is loaded into an AGO1 silencing complex from the host plant Medicago truncatula, leading to cleavage of a host target transcript encoding for the MtWRKY69 transcription factor. MtWRKY69 is downregulated in arbusculated cells in mycorrhizal roots and MtWRKY69 overexpression led to a reduced AM colonization level, indicating that its silencing is relevant for AMS. Thus, we present the first experimental evidence of the molecular mechanism of fungus to plant ckRNAi in AMS.
Physico-chemical, Heavy Metal and Microbial Analysis of Wastewater Discharged from Selected Laboratories in Bowen University, Nigeria
Authors: Akintunde Emmanuel . A. , Atobatele Bukola . O. , Akinola Omowumi . T. , Olufunmilayo Olawole , Olutona Oladele 
Published: October 2023

Abstract
The improper discharge of chemical compounds from laboratory activities into the natural environment can lead to the pollution of water resources and risks to human health. Physical, chemical, heavy metals, and microbial analyses of wastewater from selected Bowen University Laboratories were carried out to ascertain the magnitude of the effects of its pollution on entire students, staff, and the environment. The physicochemical parameters were determined using standard laboratory procedures. Concentrations of metals were determined utilizing a Flame Atomic Absorption Spectrophotometer. Quality assurance techniques involved blank determination, recovery analyses, and calibration of standards were applied. Descriptive and inferential statistics were used for data interpretations which indicated significant differences at a 95% level. The contents range of metals were: 0.345 – 7.651, 3.644 – 9.296, 54.986 – 84.749, 0.012 – 10.222, 8.032 – 12.031, and 1.001 – 23.265 µg/ml for Cu, Cr, Fe, Pb, Mn, and Zn respectively, while Cd was not detected at all in the samples. Their increase in mean concentration was in this order: Fe > Zn >Mn >Pb > Cr > Cu > Cd. Microbial analysis methods were also done where gram stain reaction, biochemical tests, and fermentation tests were used to identify the pure isolate in the sample. The work has shown that almost all physicochemical parameters monitored were either within or below the limit set by the Federal Ministry of Environmental for industrial effluent. From microbial studies, the most occurring bacteria was Protues miraculous while Enterobacter cloacae, Streptococcus sp, and Salmonella sp are the least occurring organism that has the potential to become pathogens and cause a wide range of diseases. It is therefore recommended that periodic assessment of both physicochemical, metals, and microbial analysis of the wastewater in the laboratories should be carried out, as this would be helpful in the early detection of any future degradation.
IRF5 promotes the polarization of M1 macrophages and aggravates lung inflammation in connective tissue disease-related interstitial lung disease (CTD-ILD) by regulating IFN-β
Authors: Chen Shanshan , Ming Qianwen , Wang Xiaohong 
Published: October 2023

Abstract
Background
Interferon regulatory factor 5 (IRF5) plays a vital role in proinflammatory M1 macrophages and is involved in their polarization. We aimed to explore the mechanism of action by which IRF5 participates in lung inflammation in connective tissue disease-related interstitial lung disease (CTD-ILD).
Methods
mRNA and protein levels were determined by qRT‒PCR and Western blotting. Chromatin immunoprecipitation (ChIP) assays were conducted to confirm the targeted relationship between IRF5 and interferon (IFN)-β. ELISA was performed to measure the levels of proinflammatory factors (IL-6, TNF-α, and IL-1β). IRF5 and CD86 expression levels were analyzed by flow cytometry. The nuclear location of IRF5 was detected by immunofluorescence. A double luciferase reporter assay was performed to confirm that IRF5 targets the IFN-β promoter. Hematoxylin and eosin (HE) staining was conducted to assess the histopathology of the lung.
Results
IRF5 and IFN-β levels in peripheral blood were increased in CTD-ILD patients. IRF5 enhanced IFN-β expression by directly binding to its promoter. IRF5 promoted the M1 polarization of macrophages by activating IFN-β. MS19 suppressed IFN-β expression by inhibiting the activation of IRF5. MS19 repressed the IRF5-induced promotion of LPS-treated RAW264.7 cell M1 polarization. MS19 improved lung inflammation in CTD-ILD model mice by inhibiting the polarization of M1 macrophages via the IRF5/IFN-β axis.
Conclusion
IRF5 activated IFN-β transcription by targeting the IFN-β promoter, ultimately promoting the polarization of M1 macrophages and aggravating inflammation in CTD-ILD, and the effects were reversed by MS19 treatment.
Fluid absorption during flexible ureteroscopic lithotripsy with intelligent control of renal pelvic pressure: A prospective randomized controlled trial
Authors: Deng Xiaolin , Chen Yijian , Song Leming , Du Chuance , Tan Wanlong 
Published: October 2023

Abstract
Objective: To investigate fluid absorption and its influencing factors during flexible ureteroscopic lithotripsy with intelligent control of renal pelvic pressure (RPP).
Methods: A total of 80 patients with upper urinary tract calculi underwent flexible ureteroscopic lithotripsy with intelligent control of RPP and were randomly divided into four groups. The RPP of Groups A, B, and C were set at -5 , 0 and 5 mmHg, respectively. Conventional flexible ureteroscopic lithotripsy with uncontrolled pressure served as control Group D. The perfusion flow rate was set at 100 ml/min in the four groups, with 20 patients in each group. The fluid absorption was measured by 1% ethanol every 10 minutes. Operation time, stone-free rate, and complications were recorded.
Result: Seventy-three patients were finally included in the RCT. The general and preoperative data of the patients were comparable between the groups. The fluid absorption of Groups A, B, and C was significantly less than that of Group D (P < 0.01). Fluid absorption and operation time were positively correlated, and the correlation coefficients R were 0.864, 0.896, 0.918, and 0.947, respectively (P<0.01). The fluid absorption of patients with vomiting, fever and ureteral injury was greater than that of patients without complications in the four groups (P < 0.01). In different groups, fluid absorption was greater in patients with ureteral injury PULS 1-3 than in noninjured patients (P<0.01).
CONCLUSION: Flexible ureteroscopic lithotripsy with intelligent control of RPP effectively reduces the absorption of perfusion fluid. Operation time and ureteral injury are also key factors affecting perfusion fluid absorption.
Influence of global DNA-methylation on chondrogenic fate during digit development
Authors: Chimal-Monroy Jesus , Perez-Maldonado Mario , Gonzalez-Gonzalez Ximena , Marín-Llera Jessica 
Published: October 2023

Abstract
Precise regulation of gene expression is of utmost importance during cell fate specification. DNA methylation is a key epigenetic mechanism that plays a significant role in gene expression regulation that recruits proteins involved in gene repression or inhibits the binding of transcription factors to DNA during cell commitment to regulate cell fate. Limb development is a well-established model for understanding cell fate decisions, and the formation of skeletal elements is coordinated through a sequence of events that control chondrogenesis spatiotemporally. It has been established that epigenetic control plays a crucial role in cartilage maturation. However, further investigation is required to determine its position in the earliest stages that direct cells to the chondrocyte lineage. This study investigates how the global DNA methylation environment affects cell fate divergence during the early chondrogenic events of the chondrocyte lineage. Our research has shown for the first time that inhibiting global DNA methylation in interdigital tissue with 5-azacytidine results in the formation of an ectopic digit. This discovery suggested that DNA methylation and demethylation dynamics could change the fate of cells that were supposed to die and redirect them toward chondrogenesis induction. Our vitro findings indicate that global DNA methylation is integral in regulating precartilage condensation formation by controlling cell proliferation, cell adhesion, and proapoptotic genes. As a result, the dynamics of methylation and demethylation are crucial in governing the development of chondrogenesis and cell death during digit formation.
Association between the time of initial relapse and subsequent relapses in patients with childhood-onset idiopathic nephrotic syndrome
Authors: Inoki Yuta , Nishi Kentaro , Osaka Kei , Kaneda Tomoya , Akiyama Misaki , Sato Mai , Ogura Masao , Kamei Koichi 
Published: October 2023

Abstract
Background
Nephrotic syndrome relapse within 6 months is a known risk factor for steroid-dependent nephrotic syndrome/frequently relapsing nephrotic syndrome (SDNS/FRNS), but the risk of early development of SDNS/FRNS and initiation of immunosuppression therapy remains unknown.
Methods
Patients with childhood-onset idiopathic nephrotic syndrome who had the first relapse within 6 months were enrolled. We analyzed the relationship between the time of the first and second relapse, incidence of SDNS/FRNS, and initiation of immunosuppression therapy.
Results
Forty-five patients were enrolled. Twenty out of 23 patients (87%) with a first relapse within 30 days after discontinuing initial steroid therapy experienced a second relapse within 30 days after discontinuing steroid therapy. Additionally, most patients in this group (96%) experienced a second relapse within 6 months after the onset and were diagnosed as SDNS/FRNS at this time. In this group, the incidence of SDNS/FRNS development within 6 months was 96%. In contrast, the incidence of SDNS/FRNS development within 6 months and 12 months was 18% and 41%, respectively, in patients with a first relapse more than 30 days after steroid discontinuation. The incidence of initiation of immunosuppressive agents within 6 months and 12 months was 83% and 91%, respectively, in the former group and 14% and 27%, respectively, in the latter group.
Conclusions
Most patients with a first relapse within 30 days after discontinuing steroid therapy developed SDNS/FRNS and were administered immunosuppressive agents within 6 months. Thus, it may be reasonable to start immunosuppression therapy in this group without waiting for the second relapse.
Distributed power analysis attack on SM4 encryption chip
Authors: Gong Haoran , Ju Tailiang 
Published: October 2023

Abstract
Encryption chips are specialized integrated circuits that incorporate encryption algorithms for data encryption and decryption, ensuring data confidentiality and security. In China, the domestic SM4 algorithm is commonly utilized, as opposed to the international AES encryption algorithm. These widely implemented encryption standards have been proven to be difficult to crack through crypt analysis methods Currently, power consumption side-channel attacks are the most prevalent method. They involve capturing power consumption data during the encryption process and subsequently recovering the encryption key from this data. The two leading methods are Differential Power Analysis (DPA) and machine learning techniques. DPA does not necessitate prior knowledge but relies heavily on the number of power consumption curves. With only 50 power consumption data points, theaccuracy is a mere 80%. Machine learning methods require prior knowledge, achieving an accuracy rate above 95% with only 30 power traces?, albeit with training times typically exceeding 15 minutes. In this paper, a distributed energy analysis attack approach was presented based on Correlation Power Analysis (CPA). The power consumption data was divided into 16 subsets, with each subset corresponding to 8 bytes of the key. By training each subset separately, the 8-byte key’s corresponding power consumption data is reduced to only 100 dimensions, resulting in a 76% decrease in cracking time and a 3% improvement in cracking accuracy rate.This article also trains a more complex 256 classification model to directly crack the final key, achieving a success rate of 28% in cracking 128-bit passwords with only 1 energy trace
Clinicopathological characteristics and prognosis of IgA nephropathy patients with hyperuricemia
Authors: Qi Chenchen , Liu Xudong , Mao Jing , Zhang Sen , Ye Lan , Wang Xuan , Peng Jianan , Zhou Xiaoling 
Published: October 2023

Abstract
Objective: To understand the clinical and pathological characteristics of patients with IgA nephropathy (IgAN) complicated with hyperuricemia, and to analyze the effect of serum uric acid on the prognosis of IgAN.
Methods: A retrospective analysis of 718 IgAN patients with diagnosis confirmed by renal biopsy and follow-up of more than 1 year was performed. At least two serum uric acid (SUA) levels were recorded measurements of during the follow-up period at intervals of 0.5–1 year. Time-average SUA (TA-SUA) was calculated according to the area under the curve during the follow-up period. The study’s primary end-point was doubling of creatinine or end-stage renal disease. Four groups (Q1–Q4) were divided according to baseline uric acid and TA-SUA quartile spacing from low to and the association of high uric acid level with prognosis in IgAN patients was assessed using Kaplan–Meier survival analysis and Cox proportional hazards models.
Results: Compared with the other three groups, the clinical and pathological features of patients in Q4 were more severe in both the SUA and TA-SUA groups. SBP, DBP, MAP, baseline SUA, high SUA and high TS-SUA, eGFR, ALB, 24-hour urinary protein quantification, and renal tubular atrophy/interstromal fibrosis were all identified risk factors for IgA nephropathy progression by univariate Cox regression model analysis. Multivariate results suggested that baseline SUA was not an independent risk factor for renal prognosis in IgAN patients after adjustment for clinical variables such as eGFR. High TA-SUA is an independent risk factor for renal prognosis in IgAN patients.
Conclusion: High uric acid levels IgAN patients with high uric acid levels present more severe clinical features and pathological damage. TA-SUA is as an independent risk factor for renal prognosis in IgA nephropathy patients. Baseline SUA level is not an independent risk factor for renal prognosis in IgAN patients after renal function adjustment.
A Numerical Investigation of the Thermal Behavior of Different Phase Change Materials in Thermal Energy Storage Systems
Authors: KAN Mehmet 
Published: October 2023

Abstract
Phase change materials (PCM) are widely used in thermal energy storage systems due to their high heat storage properties. However, due to the low thermal conductivity of PCMs, different surface areas are employed in order to increase the amount of energy. One of these methods is to use fins with high thermal conductivity. This study numerically investigated the thermal behavior of different PCMs (paraffin, paraffin wax, polyethylene glycol 6000) during the melting process in a thermal energy storage system with 15 fins. A FOX 50 heat flow meter was used for thermal conductivity measurements of these PCMs, and TA DSC Q200 (Differential Scanning Calorimetry) devices were used for specific heat measurements. The thermal property data of these measured PCMs were used in a time-dependent analysis. With the PCM data obtained, time-dependent thermal analyses were carried out using the Ansys-Fluent program based on the Computational Fluid Dynamics (CFD) method. The effect of these different PCMs on the melting processes was investigated by using water at 75oC in a 15-fin thermal storage system by observing their thermal behavior in the thermal energy storage system. In addition, cost analyses were conducted by determining the required amount of PCMs for the thermal storage system.
Spatial and temporal variability of soil microbial community in the Carpathian biosphere reserve
Authors: SYMOCHKO Lyudmyla , KILIÇ Kenan , DOĞAN Hakan Mete , KILIÇ Kenan 
Published: October 2023

Abstract
In this study, the effect of endogenous and exogenous factors on the soil microbiome in the primitive forest ecosystems of the Carpathian Biosphere Reserve (Shyrokoluzhansky massif) in Ukraine was been investigated as temporal variation. These unique ecosystems have a model value for investigating the distinctive soil microbiota, such as the structure of their microbial communities, the number of major ecological groups, and their spatial variability. For this aim, microbial communities in the soil were been researched in the years 2008 and 2018 by field sampling, and georeferenced field data has been collected for mapping process in geographic information systems (GIS). Microbiological studies on soils in the research area were been carried out according to the general methods used in soil microbiology. Spatial distribution of microbial communities was been transformed to interpolated raster maps for the years 2008 and 2018 by utilizing Kriging interpolation method in GIS. The relationships of soil microbial communities with elevation, climate, and soil variables were also investigated by utilizing available climate (annual mean temperature and annual precipitation amount), elevation, and soil (sand-silt-clay, organic matter, pH, and cation exchange capacity) raster maps. Statistical analyses were been conducted by utilizing test of normality (Shapiro–Wilks), descriptive statistics, t-test, correlation, and linear regression analyses. Significant increases in the number of microorganism communities were been detected from 2008 to 2018, with the most significant increases seen in oligotrophs and pedotrophs, followed by ammonifiers and bacteria. While ammonifiers and bacteria constituted the first group similar to each other in terms of the number of microorganisms, the second group was been formed by pedotrophs and oligotrophs. The differences between these two groups of microorganisms also reflected in their relationships with the independent variables. The strongest associations with microorganism communities were been found between elevation, Cation Exchange Capacity (CEC), precipitation and temperature variables. While ammonifiers and bacteria showed a negative relationship with elevation, CEC and precipitation, and a positive relationship with temperature, pedotrophs and oligotrophs showed a positive relationship with altitude, CEC and precipitation and a negative relationship with temperature. Strong relationships were been modeled. Temperature and precipitation values also increased significantly between 2008 and 2018, giving some clues about how global warming affects the geographical distribution of microorganism communities. *Corresponding author. E-mail Addres: kenankilic@ohu.edu.tr