bluuebunny/crossref_metadata_2025_split huggingface dataset:
Consistency of automated coronary calcium score and extent of emphysema with different CT scanners and radiation dose protocols in lung cancer screening
Authors: Ledda Roberta Eufrasia , Milanese Gianluca , Balbi Maurizio , Sabia Federica , Valsecchi Camilla , Ruggirello Margherita , Sverzellati Nicola , Marchianò Alfonso Vittorio , Pastorino Ugo 
Published: January 2024

AbstractObjectiveTo assess the consistency of automated measurements of coronary artery calcification (CAC) burden and emphysema extent on computed tomography (CT) images acquired with different scanners and radiation dose protocols in a lung cancer screening (LCS) population.Materials and MethodsThe analysis included two LCS cohorts, namedinter-scanner cohort, whose subjects underwent two consecutive screening rounds with two different dual-source CT scanners, andinter-dose cohort, whose subjects underwent a low-dose CT scan and an ultra-low dose CT scan.Exclusion criteria for CAC measurements were software failure, previous history of CVD and/or of coronary stenting, whereas for emphysema assessment software failure only. CT images were retrospectively analyzed by a fully automated AI software for CAC scoring, using three predefined Agatston score categories (0-99, 100-399, and ≥ 400), and emphysema quantification, using the percentage of low attenuation areas (%LAA). Demographic and clinical data were obtained from the written questionnaire completed by each participant at the first visit.Agreement for CAC and %LAA categories was measured by the k-Cohen Index with Fleiss-Cohen weights (Kw) and 95% Confidence Interval (CI).ResultsIn the inter-scanner cohort, an overlap of CAC strata was observed in 218/245 (90%) volunteers with an almost perfect agreement (Kw= 0.91, 95%CI 0.88-0.95), while an overlap of %LAA strata in 182/256 (71%) volunteers, with a substantial agreement (Kw= 0.70, 95%CI 0.63-0.76). In the inter-dose cohort, an overlap of CAC strata was observed in 275/327 (84%) volunteers, with an almost perfect agreement (Kw= 0.86, 95%CI 0.82-0.90), while an overlap of %LAA strata was found in 204/356 (57%) volunteers, with a moderate agreement (Kw= 0.57, 95%CI 0.51-0.63).ConclusionAutomated CAC and emphysema quantification showed consistent results when applied on CT images acquired with different scanners and different radiation dose CT protocols in two LCS cohorts.
Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement
Authors: Vilela-Alves Guilherme , Manuel Rita R. , Viegas Aldino , Carpentier Philippe , Biaso Frederic , Guigliarelli Bruno , Pereira Inês A. C. , Romão Maria João , Mota Cristiano 
Published: January 2024

AbstractMetal-dependent formate dehydrogenases are very promising targets for enzyme optimization and design of bio-inspired catalysts for CO2reduction, towards novel strategies for climate change mitigation. For effective application of these enzymes, the catalytic mechanism must be fully understood, and the molecular determinants clarified. Despite numerous studies, several doubts persist, namely regarding the role played by the possible dissociation of the SeCys ligand from the Mo/W active site. Additionally, the O2sensitivity of these enzymes must also be understood as it poses an important obstacle for biotechnological applications. Here we present a combined biochemical, spectroscopic, and structural characterization ofDesulfovibrio vulgarisFdhAB (DvFdhAB) when exposed to oxygen in the presence of a substrate (formate or CO2). This study reveals that O2inactivation is promoted by the presence of either substrate and involves forming a new species in the active site, captured in the crystal structures, where the SeCys ligand is displaced from tungsten coordination and replaced by a dioxygen or peroxide molecule. This new form was reproducibly obtained and supports the conclusion that, although W-DvFdhAB can catalyze the oxidation of formate in the presence of oxygen for some minutes, it gets irreversibly inactivated after prolonged O2exposure in the presence of either substrate. These results reveal that oxidative inactivation does not require reduction of the metal, as widely assumed, as it can also occur in the oxidized state in the presence of CO2.
Measuring and modeling the dynamics of mitotic error correction
Authors: Ha Gloria , Dieterle Paul , Shen Hao , Amir Ariel , Needleman Daniel J. 
Published: January 2024

SummaryError correction is central to many biological systems and is critical for protein function and cell health. During mitosis, error correction is required for the faithful inheritance of genetic material. When functioning properly, the mitotic spindle segregates an equal number of chromosomes to daughter cells with high fidelity. Over the course of spindle assembly, many initially erroneous attachments between kinetochores and microtubules are fixed through the process of error correction. Despite the importance of chromosome segregation errors in cancer and other diseases, there is a lack of methods to characterize the dynamics of error correction and how it can go wrong. Here, we present an experimental method and analysis framework to quantify chromosome segregation error correction in human tissue culture cells with live cell confocal imaging, timed premature anaphase, and automated counting of kinetochores after cell division. We find that errors decrease exponentially over time during spindle assembly. A coarse-grained model, in which errors are corrected in a chromosome autonomous manner at a constant rate, can quantitatively explain both the measured error correction dynamics and the distribution of anaphase onset times. We further validated our model using perturbations that destabilized microtubules and changed the initial configuration of chromosomal attachments. Taken together, this work provides a quantitative framework for understanding the dynamics of mitotic error correction.
A toxic environment selects for specialist microbiome in poison frogs
Authors: Caty Stephanie N. , Alvarez-Buylla Aurora , Vasek Cooper , Tapia Elicio E. , Martin Nora A. , McLaughlin Theresa , Weber Peter K. , Mayali Xavier , Coloma Luis A. , Morris Megan M. , O’Connell Lauren A. 
Published: January 2024

SummaryShifts in microbiome community composition can have large effects on host health. It is therefore important to understand how perturbations, like those caused by the introduction of exogenous chemicals, modulate microbiome community composition. In poison frogs within the family Dendrobatidae, the skin microbiome is exposed to the alkaloids that the frogs sequester from their diet and use for defense. Given the demonstrated antimicrobial effects of these poison frog alkaloids, these compounds may be structuring the skin microbial community. To test this, we first characterized microbial communities from chemically defended and closely related non-defended frogs from Ecuador. Then we conducted a laboratory experiment to monitor the effect of the alkaloid decahydroquinoline (DHQ) on the microbiome of a single frog species. In both the field and lab experiments, we found that alkaloid-exposed microbiomes are more species rich and phylogenetically diverse, with an increase in rare taxa. To better understand the strain-specific behavior in response to alkaloids, we cultured microbial strains from poison frog skin and found the majority of strains exhibited either enhanced growth or were not impacted by the addition of DHQ. Additionally, stable isotope tracing coupled to nanoSIMS suggests that some of these strains are able to metabolize DHQ. Taken together, these data suggest that poison frog chemical defenses open new niches for skin-associated microbes with specific adaptations, including the likely metabolism of alkaloids, that enable their survival in this toxic environment. This work helps expand our understanding of how exposure to exogenous compounds like alkaloids can impact host microbiomes.
Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decay
Authors: Kolakada Divya , Fu Rui , Biziaev Nikita , Shuvalov Alexey , Lore Mlana , Campbell Amy E. , Cortázar Michael A. , Sajek Marcin P. , Hesselberth Jay R. , Mukherjee Neelanjan , Alkalaeva Elena , Coban Akdemir Zeynep H. , Jagannathan Sujatha 
Published: January 2024

ABSTRACTNonsense variants underlie many genetic diseases. The phenotypic impact of nonsense variants is determined by nonsense-mediated mRNA decay (NMD), which degrades transcripts with premature termination codons (PTCs). Despite its clinical importance, the factors controlling transcript-specific and context-dependent variation in NMD activity remain poorly understood. Through analysis of human genetic datasets, we discovered that the amino acid preceding the PTC strongly influences NMD activity. Notably, glycine codons promote robust NMD efficiency and show striking enrichment before PTCs but depletion before normal termination codons (NTCs). This glycine-PTC enrichment is particularly pronounced in genes tolerant to loss-of-function variants, suggesting evolutionary selection or neutrality conferred by efficient elimination of truncated proteins from non-essential genes. Using biochemical assays and massively parallel reporter analysis, we demonstrated that the peptide release rate during translation termination varies substantially with the identity of the preceding amino acid and serves as the primary determinant of NMD activity. We propose a “window of opportunity” model where translation termination kinetics modulate NMD efficiency. By revealing how sequence context shapes NMD activity through translation termination dynamics, our findings provide a mechanistic framework for improved clinical interpretation of nonsense variants.
Evolution and diversification of the momilactone biosynthetic gene cluster in the genusOryza
Authors: Priego-Cubero Santiago , Toyomasu Tomonobu , Gigl Michael , Liu Youming , Hasegawa Yuto , Nojiri Hideaki , Dawid Corinna , Okada Kazunori , Becker Claude 
Published: January 2024

ABSTRACTPlants are master chemists and collectively are able to produce hundreds of thousands of different organic compounds. The genes underlying the biosynthesis of many specialized metabolites are organized in biosynthetic gene clusters (BGCs), which is hypothesized to ensure their faithful co-inheritance and to facilitate their coordinated expression. In rice, momilactones are diterpenoids that act in plant defence and various organismic interactions. Many of the genes essential for momilactone biosynthesis are grouped in a BGC. Here, we apply comparative genomics of diploid and allotetraploidOryzaspecies to reconstruct the species-specific architecture, evolutionary trajectory, and sub-functionalisation of the momilactone biosynthetic gene cluster (MBGC) in theOryzagenus. Our data show that the evolution of the MBGC is marked by lineage-specific rearrangements and gene copy number variation, as well as by occasional cluster loss. We identified a distinct cluster architecture inO. coarctata, which represents the first instance of an alternative architecture of the MBGC inOryzaand strengthens the idea of a common origin of the cluster inOryzaand the distantly related genusEchinochloa. Our research illustrates the evolutionary and functional dynamics of a biosynthetic gene cluster within a plant genus.
Multimodal interactions in Stomoxys navigation reveals synergy between olfaction and vision
Authors: Getahun Merid N , Baleba Steve , Ngiela John , Ahuya Peter , Masiga Daniel 
Published: January 2024

AbstractStomoxys flies exhibit an attraction towards objects that offer no rewards, such as traps and targets devoid of blood or nectar incentives. This behavior provides an opportunity to develop effective tools for vector control and monitoring. However, for these systems to be sustainable and eco-friendly, the visual cues used must be selective in attracting the target vector(s). In this study, we modified the existing blue Vavoua trap, originally designed to attract biting flies, to create a deceptive host attraction system specifically biased towards attracting Stomoxys. Our research reveals that Stomoxys flies are attracted to various colors, with red proving to be the most attractive and selective color for Stomoxys compared to other colors tested. Interestingly, our investigation on cattle-Stomoxys interaction demonstrates that Stomoxys flies do not have preference for a specific livestock fur color phenotype, despite variation in spectrum. To create a realistic sensory impression of the trap in the Stomoxys nervous system, we incorporated olfactory cues from livestock host odors that significantly increased trap catches. The optimized novel nanopolymer bead dispenser capable of effectively releasing the attractive odor, carvone + p-cresol, with strong plume strands, longevity. Overall, red trap baited with nano polymer beads dispenser is environmentally preferred.
Taking aim at the wino-Higgsino plane with the LHC
Authors: Carpenter Linda M. , Gilmer Humberto , Kawamura Junichiro , Murphy Taylor 
Published: January 2024

In this work we explore multiple search strategies for Higgsinos and mixed Higgsino-wino states in the minimal supersymmetric Standard Model (MSSM) and project the results onto the (μ,M2) plane. Assuming associated production of Higgsino-like pairs with a W/Z boson, we develop a search in a channel characterized by a hadronically tagged vector boson accompanied by missing energy. We use as our template an ATLAS search for dark matter produced in association with a hadronically decaying vector boson, but upgrade the search by implementing a joint-likelihood analysis, binning the missing transverse energy distribution, which greatly improves the search sensitivity. For Higgsino-like states (more than 96% admixture) we find sensitivity to masses up to 550 GeV. For well-mixed Higgsino-wino states (70–30% Higgsino) we still find sensitivities above 300 GeV. Using this newly proposed search, we draw a phenomenological map of the wino-Higgsino parameter space, recasting several complementary searches for disappearing tracks, soft leptons, trileptons, and hadronic diboson events in order to predict LHC coverage of the (μ,M2) mass plane at integrated luminosities of up to 3  ab−1. Altogether, the full run of the HL-LHC can exclude much of the “natural” (μ,M2<500  GeV) wino-Higgsino parameter space.

Published by the American Physical Society
2024
Placebo effect in food marketing: young consumers' perception of food healthiness and tastiness in reduced-sugar labeling
Authors: Huang Li , Song Xi , Liu Matthew Tingchi , Chang Wen-yu , Shi Guicheng James 
Published: January 2024

PurposeThe purpose of this study is to provide a nuanced understanding of the marketing placebo effect (MPE) of products with reduced sugar labeling and how it forms certain perceptual underpins (perceived healthiness (PH) and perceived tastiness (PT)), with the potential effect of product category and social class in consideration.Design/methodology/approachThe proposed model is tested using a sample of 822 participants by employing partial least squares structural equation modeling (PLS-SEM). Hypothetical relationships among MPE, PH, PT, purchase intention (PI) and social class are examined for both hedonic and utilitarian products.FindingsThe results highlight the positive role of MPE in leveraging consumer PI through the parallel mediation of PH and PT. The positive effect of MPE on PH and PT was more pronounced for the utilitarian product. In addition, social class negatively moderated the relationship between PH and PI only in the case of the utilitarian product.Originality/valueThis paper contributed to the MPE literature in the food industry by challenging the conventional intuition of “Unhealthy = Tasty” and highlighting the potential of perceived food healthiness to positively influence perceived food tastiness under the effect of MPE. An upper social class would attenuate the positive effect of perceived food healthiness on PI.
Let us buy online directly from farmers: an integrated framework of individualistic and collectivistic consumption values
Authors: Kol Ofrit , Zimand-Sheiner Dorit , Levy Shalom 
Published: January 2024

PurposeBuying directly from farmers online has become increasingly popular in recent years. This study aims to investigate the effect of the interaction between various consumption values that drive consumers to buy directly from farmers online. The proposed conceptual framework suggests that consumers who buy online directly from farmers are driven by an interaction of weighted individualistic consumption value (i.e. an integration of values such as saving money, getting quality and fresh produce) and collectivistic values (pro-environmental behaviour and ethnocentric perception).Design/methodology/approachData were collected using a representative sample of 576 consumers via an online access panel and analysed using AMOS SEM.FindingsA weighted individualistic consumption value affects consumer attitudes and, consequently, consumers' intention to buy agri-food products directly from farmers. Nonetheless, individualistic consumption value is more effective in enhancing attitudes among consumers with high pro-environmental behaviour. Moreover, ethnocentric perception lowers the effect of individualistic consumption value on attitudes and enhances the positive effect of attitudes on buying intention.Originality/valueThis study contributes to the literature on consumer online behaviour when buying food products directly from farmers. Its originality lies in the effect of interacting individualistic and collectivistic consumption values to explain consumer motivation for this behaviour.
Enhancing fire safety with Improved Risk Index and BIM in building evacuation
Authors: Zhang Jingqi , Jiang Shaohua , Qi Xiaomin 
Published: January 2024

PurposeThe purpose of this paper is to conduct a comprehensive study on building, fire and evacuation, so as to effectively improve the efficiency of building fire evacuation and the management level of fire evacuation site. Make up for the difficulties of BIM technology in effectively connecting building information and fire data.Design/methodology/approachFirst, this paper establishes a fire model and an evacuation model based on BIM information. Then, the safety index (SI) is introduced as a comprehensive index, and the IRI is established by integrating the SI function to evaluate the safety of evacuation routes. Based on these two indices, the IRI-based fire evacuation model is established.FindingsThis study offers an Improved Risk Index (IRI)-based fire evacuation model, which may achieve effective evacuation in fire scenes. And the model is verified by taking the fire evacuation of a shopping center building as an example.Originality/valueThis paper proposes a fire evacuation principle based on IRI, so that the relevant personnel can comprehensively consider the fire factors and evacuation factors to achieve the optimization of building design, thereby improving the fire safety of buildings.
The intention-behaviour gap in sustainable hospitality: a critical literature review
Authors: Khan Nayla , Acuti Diletta , Lemarie Linda , Viglia Giampaolo 
Published: January 2024

Purpose
The intention of consumers to behave sustainably is not a reliable predictor of sustainable hospitality choices. This intention-behaviour gap represents one of the biggest challenges for marketers and environment-friendly businesses. To address this issue, this study aims to draw upon the intention-behaviour gap. The authors revise the sustainable hospitality literature to identify the limitations, to evaluate the extent to which the intention-behaviour gap is embedded in the hospitality literature and to provide practical guidance on how to move research forward in the sustainable hospitality field.

Design/methodology/approach
The authors adopted a five-step process to review and analyse 71 scientific papers published in 14 Hospitality Journals. The authors developed a descriptive overview of the literature showing the publications in this field over the years, the sustainability practices implemented by companies and consumers and the setting of the studies. Finally, the authors conducted a critical analysis of research in sustainable hospitality adopting the intention-behaviour gap lens.

Findings
Leveraging the descriptive overview and critical analysis, the authors offer four directions for future research to address the existing literature limitations. The authors encourage scholars to expand the scope of the research setting, investigate diverse sustainability practices, integrate existing knowledge on the intention-behaviour gap into sustainable hospitality research and combine traditional research methods with emerging technologies.

Practical implications
This study exposes the theoretical challenge of applying conventional behaviour theories to sustainable hospitality, prompting a call for framework re-evaluation. It offers practical insights, empowering researchers, marketers and policymakers to navigate and mitigate the intention-behaviour gap in sustainable hospitality.

Originality/value
The originality of this paper is underscored by its distinctive focus on the unique intention-behaviour gap within sustainable hospitality, coupled with a compelling call to re-evaluate traditional behavioural frameworks. It provides a roadmap for future research in sustainable hospitality, benefiting researchers, policymakers and marketers in promoting sustainable initiatives.
Leveraging organizational knowledge to develop agility and improve performance: the role of ambidexterity
Authors: Stei Gerald , Rossmann Alexander , Szász Levente 
Published: January 2024

PurposeAs a response to the increased frequency of disruptive events and intense competition, organizational agility has become a key concept in organizational research. Fostering organizational agility requires leveraging knowledge that exists both outside (exploration) and inside (exploitation) the organization. This research tests the so-called ambidexterity hypothesis, which claims that a balance between exploration and exploitation leads to increased organizational outcomes, including the development of organizational agility. Complementing previously established measurement models on ambidexterity, this research proposes an alternative measurement model to analyze how ambidexterity can enhance organizational agility and, indirectly, performance, taking into consideration the moderating effect of environmental competitiveness.Design/methodology/approachA review of existing measurement models for ambidexterity shows that tension, a crucial aspect of ambidexterity, is often neglected. The authors, therefore, develop a new measurement model of ambidexterity to incorporate ambidexterity-induced tension. Using this measurement model, they examine the effect of ambidexterity on the development of entrepreneurial and adaptive agility as well as performance.FindingsAmbidexterity positively influences both entrepreneurial and adaptive agility, indicating that a balance between exploration and exploitation has superior organizational effects. This finding confirms the ambidexterity hypothesis with respect to organizational agility. Furthermore, both entrepreneurial and adaptive agility drive organizational performance. These two indirect effects via agility fully mediate the impact of ambidexterity on organizational performance. Finally, environmental competitiveness positively moderates the relationship between ambidexterity and adaptive agility.Originality/valueThe findings extend research on ambidexterity by showing its positive effects on organizational agility. Furthermore, the study proposes an alternative operationalization to capture the ambidexterity construct that may lay the groundwork for further applications of the ambidexterity concept.