bluuebunny/crossref_metadata_2025_split huggingface dataset:
Make my memory: How advertising can change our memories of the past
Authors: Braun Kathryn A. , Ellis Rhiannon , Loftus Elizabeth F. 
Published: August 2002

AbstractMarketers use autobiographical advertising as a means to create nostalgia for their products. This research explores whether such referencing can cause people to believe that they had experiences as children that are mentioned in the ads. In Experiment 1, participants viewed an ad for Disney that suggested that they shook hands with Mickey Mouse as a child. Relative to controls, the ad increased their confidence that they personally had shaken hands with Mickey as a child at a Disney resort. The increased confidence could be due to a revival of a true memory or the creation of a new, false one. In Experiment 2, participants viewed an ad for Disney that suggested that they shook hands with an impossible character (e.g., Bugs Bunny). Again, relative to controls, the ad increased confidence that they personally had shaken hands with the impossible character as a child at a Disney resort. The increased confidence is consistent with the notion that autobiographical referencing can lead to the creation of false or distorted memory. © 2002 John Wiley & Sons, Inc.
The chemopreventive compound curcumin is an efficient inhibitor of Epstein‐Barr virus BZLF1 transcription in Raji DR‐LUC cells*
Authors: Hergenhahn Manfred , Soto Ubaldo , Weninger Annette , Polack Axel , Hsu Chih‐Hung , Cheng Ann‐Lii , Rösl Frank 
Published: August 2002

AbstractTo characterize the effects of inhibitors of Epstein‐Barr virus (EBV) reactivation, we established Raji DR‐LUC cells as a new test system. These cells contain the firefly luciferase (LUC) gene under the control of an immediate‐early gene promoter (duplicated right region [DR]) of EBV on a self‐replicating episome. Luciferase induction thus serves as an intrinsic marker indicative for EBV reactivation from latency. The tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) induced the viral key activator BamH fragment Z left frame 1 (BZLF1) protein (“ZEBRA”) in this system, as demonstrated by induction of the BZLF1 protein‐responsive DR promoter upstream of the luciferase gene. Conversely, both BZLF1 protein and luciferase induction were inhibited effectively by the chemopreventive agent curcumin. Semiquantitative reverse transcriptase (RT)‐polymerase chain reaction (PCR) further demonstrated that the EBV inducers TPA, sodium butyrate, and transforming growth factor‐β (TGF‐β) increased levels of the mRNA of BZLF1 mRNA at 12, 24, and 48 h after treatment in these cells. TPA treatment also induced luciferase mRNA with similar kinetics. Curcumin was found to be highly effective in decreasing TPA‐, butyrate‐, and TGF‐β‐induced levels of BZLF1 mRNA, and of TPA‐induced luciferase mRNA, indicating that three major pathways of EBV are inhibited by curcumin. Electrophoretic mobility shift assays (EMSA) showed that activator protein 1 (AP‐1) binding to a cognate AP‐1 sequence was detected at 6 h and could be blocked by curcumin. Protein binding to the complete BZLF1 promoter ZIII site (ZIIIA+ZIIIB) demonstrated several specific complexes that gave weak signals at 6 h and 12 h but strong signals at 24 h, all of which were reduced after application of curcumin. Autostimulation of BZLF1 mRNA induction through binding to the ZIII site at 24 h was confirmed by antibody‐induced supershift analysis. The present results confirm our previous finding that curcumin is an effective agent for inhibition of EBV reactivation in Raji DR‐CAT cells (carrying DR‐dependent chloramphenicol acetyltransferase), and they show for the first time that curcumin inhibits EBV reactivation mainly through inhibition of BZLF1 gene transcription. © 2002 Wiley‐Liss, Inc.
Abnormalities of the spatiotemporal characteristics of gait at the onset of freezing in Parkinson's disease
Authors: Nieuwboer Alice , Dom René , De Weerdt Willy , Desloovere Kaat , Fieuws Steffen , Broens‐Kaucsik Eva 
Published: August 2002

AbstractWe investigated the spatiotemporal variables of gait leading up to freezing. Gait analysis was carried out on 14 patients with Parkinson's disease in the off phase of the medication cycle. A computerised, three‐dimensional gait analysis system was used to measure the walking pattern. After several trials of normal walking with voluntary stopping, distracting manoeuvres and obstacles on the walkway were used to provoke freezing or festination. The gait variables of normal (off phase), festinating, prestop, and prefreezing strides were analysed using analysis of variance for repeated‐measures. Cadence was excessively increased (68%) and stride length decreased (69%) during festination compared with normal off walking; a pattern which remained pronounced when comparing prefreezing strides with normal stopping. Analysing in more detail the three steps before a freeze, we found a progressive decrease of stride length and stable cadence rates and proportions of double support phases. The relationship between cadence and stride length exhibited an exponential increase of cadence with a decreasing stride length during festination and freezing. Results suggest that freezing is caused by a combination of an increasing inability to generate stride length superimposed on a dyscontrol of the cadence of walking. © 2001 Movement Disorder Society.
Objective tinnitus associated with essential laryngeal myoclonus: Report of two cases
Authors: Bertholon Pierre , Convers Philippe , Antoine Jean Christophe , Mayaud Régis , Prades Jean Michel , Michel Daniel , Martin Christian 
Published: August 2002

AbstractWe report on two patients with an objective tinnitus. In the first one, the objective tinnitus was unique as it was due to bilateral rhythmic contractions of the vocal cords. In the second, the objective tinnitus arose from Eustachian tube contractions and was associated with up and down movements of the larynx. In both patients, the abnormal laryngeal movement shared most of the characteristics of palatal or palatolaryngeal myoclonus and was thought to be laryngeal myoclonus. Its origin remained essential as all the investigations were negative. © 2001 Movement Disorder Society.
Transferrin/transferrin receptor‐mediated drug delivery
Authors: Li Hongyan , Qian Zhong Ming 
Published: August 2002

AbstractSince transferrin was discovered more than half a century ago, a considerable effort has been made towards understanding tranferrin‐mediated iron uptake. However, it was not until recently with the identification and characterization of several new genes related to iron homeostasis, such as the hemochromatosis protein HFE and the iron transporter DMT1, that our knowledge has been advanced dramatically. A major pathway for cellular iron uptake is through internalization of the complex of iron‐bound transferrin and the transferrin receptor, which is negatively modulated by HFE, a protein related to hereditary hemochromatosis. Iron is released from transferrin as the result of the acidic pH in endosome and then is transported to the cytosol by DMT1. The iron is then utilized as a cofactor by heme and ribonucleotide reductase or stored in ferritin. Apart from iron, many other metal ions of therapeutic and diagnostic interests can also bind to transferrin at the iron sites and their transferrin complexes can be recognized by many cells. Therefore, transferrin has been thought as a “delivery system” for many beneficial and harmful metal ions into the cells. Transferrin has also be widely applied as a targeting ligand in the active targeting of anticancer agents, proteins, and genes to primary proliferating malignant cells that overexpress transferrin receptors. This is achieved by conjugation of transferrin with drugs, proteins, hybride systems with marcomolecules and as liposomal‐coated systems. Conjugates of anticancer drugs with transferrin can significantly improve the selectivity and toxicity and overcome drug resistance, thereby leading to a better treatment. The coupling of DNA to transferrin via a polycation such as polylysine or via cationic liposomes can target and transfer of the extrogenous DNA particularly into proliferating cells through receptor‐mediated endocytosis. These kinds of non‐viral vectors are potential alternatives to viral vectors for gene therapy, if the transfection efficiency can be improved. Moreover, transferrin receptors have shown potentials in delivery of therapeutic drugs or genes into the brain across blood–brain barrier. © 2002 Wiley Periodicals, Inc. Med Res Rev, 22, No. 3, 225–250, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/med.10008
Dynamic rectus abdominis muscle flap for intestinal stomal continence: A systematic approach
Authors: Stadelmann Wayne K. , Bardoel Janou W.J.M. , Perez‐Abadia Gustavo , Majzoub Ramsey K. , Maldonado Claudio , Tobin Gordon R. , Kon Moshe , Barker John H. 
Published: August 2002

AbstractSeveral attempts to create a continent stomal sphincter using dynamic myoplasty with limited success have been reported. Denervation atrophy and early muscle fatigue have plagued all reported attempts to make a continent stoma a reality. To address this problem in a series of experiments, we designed a stomal sphincter using the most caudal segment of the rectus abdominis muscle. Then, we performed a study to determine whether a sphincter created with a rectus abdominis muscle island flap could maintain stomal continence in the short term. We found that when stimulated using two different electrical stimulation protocols, in all cases the rectus abdominis muscle sphincter generated peak pressures well above those needed to maintain stomal continence (60 mm Hg). All sphincters were able to maintain stomal continence at all intraluminal bowel pressures tested. We found one of these protocols to be far superior and reached 4 hours of stomal continence after 8 to 10 weeks of electrical stimulation. © 2001 Wiley‐Liss, Inc. MICROSURGERY 21:248–255 2001
Multi‐component behavioral intervention to promote health protective behaviors in childhood cancer survivors: The Protect Study
Authors: Hudson Melissa M. , Tyc V.L. , Srivastava D.K. , Gattuso J. , Quargnenti A. , Crom D.B. , Hinds P. 
Published: August 2002

AbstractBackgroundImproved cure rates for childhood cancer have produced a growing population of survivors at risk for late toxicities of chemotherapy and radiation therapy. Healthy behaviors can modify these risks. We initiated a controlled prospective trial to determine if a multi‐component behavioral intervention could induce change in childhood cancer survivors' health knowledge, health perceptions, and practice of health‐protective behaviors.ProcedureAdolescent cancer survivors attending a long‐term follow‐up clinic were randomized to receive standard follow‐up care or standard care plus the educational intervention. Baseline measures were obtained at randomization (T0) and repeated 1 year (T1) later during the survivors' annual check‐up.ResultsOf 272 patients enrolled and randomized, 251 are evaluable at both time points. Treatment and control groups were similar in regards to diagnosis, gender, race, and age. The change in outcome measures over the year (T1−T0) was not significantly different between the two groups as assessed by a two‐sample pooled t test. However, additional exploratory analyses indicated a significant gender difference in knowledge with female survivors in the intervention group having higher scores. In addition, patients who choose certain individual health goals, such as breast/testicular self‐examination, showed improved practice of the health behavior. In addition, in a very exploratory analysis, a gender difference in response to the intervention was noted, with females exhibiting a greater improvement in knowledge scores than did males.ConclusionsAlthough the multi‐behavioral educational intervention did not induce change in health knowledge, perceptions, and behaviors of childhood cancer survivors for the treatment group as a whole, gender differences and specific health goal differences were found. These findings suggest that future interventions should be tailored to reflect gender differences and the nature of the health goal under assessment. Med Pediatr Oncol 2002;39:2–11. © 2002 Wiley‐Liss, Inc.
ACCORD‐ADEQUATE: an improved technique for the acquisition of inverse‐detected INADEQUATE data
Authors: Thomas Williamson R. , Marquez Brian L. , Gerwick William H. , Koehn Frank E. 
Published: August 2002

AbstractA new approach for the acquisition of 1H‐detected INADEQUATE‐type data is presented. This method uses a decremented variable delay for the evolution of 1J(C,C) couplings. It is demonstrated that this variation of the 1,1‐ADEQUATE experiment provides superior performance when compared with the normal static‐optimized 1,1‐ADEQUATE experiment. This improvement is manifested in both the number and quality of correlations present in the processed data. The technique was validated using the model compound ethyl trans‐crotonate and its utility was demonstrated utilizing the structurally unique marine natural product jamaicamide A. Copyright © 2001 John Wiley & Sons, Ltd.
Structural and 1H and 13C NMR analysis of two new glabretal triterpenoid derivatives from Guarea jamaicensis
Authors: Harding Wayne W. , Jacobs Helen , McLean Stewart , Reynolds William F. 
Published: August 2002

AbstractThe 1H and 13C NMR spectra of the glabretal derivatives, 21,24‐epoxy‐3α, 7α, 21,23‐tetraacetoxy‐25‐hydroxy‐4α,4β,8β‐trimethyl‐14,18‐cyclo‐5α,13α,14α,17α‐cholestane and 21,23‐epoxy‐3α,7α,21,24,25‐pentaacetoxy‐4α,4β,8β‐trimethyl‐14,18‐cyclo‐5α,13α,14α,17α‐cholestane, obtained from an acetylated fraction of extracts of Guarea jamaicensis, were completely assigned. 1D NMR and a number of 2D shift correlated NMR experiments, 1H,1H‐COSY, 1H,13C‐HSQC‐1J(C,H), 1H,13C‐HMBC nJ(C,H) (n = 2 and 3) and 2D 1H,1H‐NOESY, were utilized. Copyright © 2001 John Wiley & Sons, Ltd.
Efficient method for expressing transgenes in nonhuman primate embryos using a stable episomal vector*
Authors: Wolfgang Michael J. , Marshall Vivienne S. , Eisele Stephen G. , Schotzko Michele L. , Thomson James A. , Golos Thaddeus G. 
Published: August 2002

AbstractTransgenesis in the nonhuman primate can enhance the study of human biology by providing animal models for the study of primate‐specific physiology, pathophysiology, and embryonic development. Progress with this technology has been hindered by the inherent inefficiency of transgenesis, transgene silencing, and practical restrictions on the production of sufficient pronuclear stage nonhuman primate zygotes. We have developed a novel technique using an Epstein Barr virus (EBV)‐based episomal vector to produce rhesus monkey (Macaca mulatta) embryos expressing a transgene. Plasmid DNA containing the latent origin of replication, oriP, and Epstein Barr Nuclear Antigen‐1 (EBNA‐1) of EBV, as well as a CMV IE‐enhanced green fluorescent protein (eGFP) expression cassette, was introduced into rhesus embryos by direct pronuclear microinjection. We detected eGFP in early cleavage stage embryos (4–8 cell) and throughout the duration of culture (day 8–9 blastocysts) by epifluorescent microscopy. A 50% transduction rate was obtained with the EBV‐based vector. Microinjected embryos expressed eGFP and retained their developmental capacity as evidenced by development to the blastocyst stage. EBV‐based vectors present a novel and efficient means of delivering transgenes for the study of the molecular control of primate embryonic development. Mol. Reprod. Dev. 62: 69‐73, 2002. © 2002 Wiley‐Liss, Inc.
DNA hypomethylation of karyoplasts for bovine nuclear transplantation*
Authors: Jones Karen L. , Hill Jonathon , Shin Tae Young , Lui Ling , Westhusin Mark 
Published: August 2002

AbstractThe objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum‐fed (SF), serum‐starved (SS), and 1, or 5 μM 5‐azacytidine (5‐aza‐CR) treated cells was digested with a methylation sensitive enzyme, and evaluated for DNA methylation. A significant reduction in DNA methylation was observed in cells cultured for 48 or 72 hr in SS medium as well as in cells cultured for 48 hr in the presence of 5 μM 5‐aza‐CR when compared to cells cultured in SF medium. All other comparisons contained no significant differences when compared to controls. When donor cells were cultured in 5‐aza‐CR, SF, or SS treatment media for 48 hr, no significant difference was observed (P = 0.06) in blastocyst development rates after NT. One embryo produced by donor cells treated with 5‐aza‐CR established a pregnancy. Four pregnancies resulted from embryos produced by SS donor cell NT and 3 resulted from embryos produced by SF donor cell NT. Supplementation of the donor cell culture medium with 5‐aza‐CR was not beneficial for increasing blastocyst rate or establishing pregnancy after NT. Mol. Reprod. Dev. 60: 208–213, 2001. © 2001 Wiley‐Liss, Inc.
Exocytosis of a 60 kDa protein (Calreticulin) from activated hamster oocytes
Authors: Muñoz‐Gotera Rafaela J. , Hernández‐González Enrique O. , Mendoza‐Hernández Guillermo , Contreras Rubén G. , Mújica Adela 
Published: August 2002

AbstractThe sp50 protein localized at the acrosomal region of guinea pig sperm was suggested to participate in acrosome exocytosis, the acrosome reaction (AR). On the other hand, the cortical reaction (CR), also an exocytotic event, occurs during egg activation. The aim of the present work was to identify sp50 and also to define if sp50 is present in hamster eggs, as well as its location before and after CR. Sp50 was identified as calreticulin (CRT), based on: (a) its NH2‐terminal amino acid (25 aa) sequence, (b) a cross‐recognition of pure sp50 and pure CRT with anti‐CRT (from Santa Cruz, anti‐CRTsc), and anti‐sp50 (anti‐sp50/CRT) antibodies, respectively, and (c) that both antibodies revealed a 50 kDa protein in a Brij sperm extract. On the other hand, CRT presence in eggs was positively determined by Western blotting (Wb) using anti‐sp50/CRT antibody which recognized a 60 kDa protein in the egg extract, and by indirect immunofluorescence (IIF), CRT was located in the cortical granules (CG). It was defined by a granular pattern and co‐localization with mannose, a specific carbohydrate of the CG. Additionally, a decrease in CRT concentration occurred in eggs after their activation and, in parallel, the protein was revealed in the egg's incubation medium. In activated eggs with zona pellucida (ZP), CRT remains as a halo in the perivitelline space and around the polar body. From these results we suggest that: (1) CRT is present in the CG of non‐activated hamster eggs, (2) CRT is exocytosed during the CR, in response to egg activation, and (3) CRT might participate in the block to polyspermy, together with other CG components. Mol. Reprod. Dev. 60:405‐413, © 2001 Wiley‐Liss, Inc.
Effect of antibodies to sperm‐specific recombinant contraceptive vaccinogen (rCV) on murine fertilization: search for an animal model to examine its contraceptive potential
Authors: Chauhan Subhash C. , Naz Rajesh K. 
Published: August 2002

AbstractRecently, we cloned and sequenced a sperm‐specific antigen, designated as Contraceptive Vaccinogen (rCV), from human testis (Naz et al., 2001). The present study was conducted to examine its proteomic homologue and function in murine sperm, in order to find out whether or not the mouse can provide a suitable model for examining its immunocontraceptive effects. This was examined by using purified antibodies (Ab) raised against the recombinant (r) human CV antigen of ∼44 kD. In the Western blot procedure, rCV antibodies recognized a specific protein band of ∼64 ± 5 kD in murine testis and murine sperm extracts, the band similar to that found in human testis and human sperm. In the immunoprecipitation procedure, rCV Ab immunoprecipitated a protein band of similar size from murine sperm and murine testis extracts. The immunocytochemical (ICT), immunoscanning electronmicroscopic (ISEM) and the immunobead binding technique (IBT) revealed the subcellular localization of CV antigen on the surface of acrosome and tail regions of the noncapacitated and capacitated murine sperm cell. In functional bioassays, rCV Ab inhibited the acrosome reaction as well as sperm–egg binding in vitro. These data indicate that the CV antigen is expressed in murine sperm and has a biological role in sperm function and sperm–egg binding. In vitro inhibition of capacitation/acrosome reaction and sperm–zona binding suggest that the mouse can provide a suitable model to examine the immunocontraceptive effects of CV antigen in actively‐immunized animals. Mol. Reprod. Dev. 60:425‐432, © 2001 Wiley‐Liss, Inc.
Sulfogalactosylglycerolipid is involved in human gamete interaction
Authors: Weerachatyanukul Wattana , Rattanachaiyanont Manee , Carmona Euridice , Furimsky Anna , Mai Andrew , Shoushtarian Ali , Sirichotiyakul Supatra , Ballakier Hanna , Leader Arthur , Tanphaichitr Nongnuj 
Published: August 2002

AbstractRecent results from our laboratory have revealed the role of sulfogalactosylglycerolipid (SGG) in mouse sperm‐zona pellucida (ZP) binding. In this report, we demonstrated the presence of SGG in Percoll‐gradient centrifuged (PGC) human sperm by high performance thin layer chromatography with orcinol and Azure A staining, specific for glycolipids and sulfolipids, respectively. SGG in human PGC sperm was quantified by its affinity to Azure A to be 12–15 mol% of sperm lipids. Indirect immunofluorescence revealed that SGG existed on both live and aldehyde fixed human sperm in the head region. Pretreatment of human PGC sperm with affinity purified antiSGG Fab markedly inhibited sperm binding to the ZP in a concentration dependent manner, without any changes in the spontaneous acrosome rate or sperm motility parameters. Fluorescently labeled SGG liposomes also bound uniformly to isolated human ZP, while fluorescently labeled galactosylglycerolipid (GG, SGG's parental lipid) or phosphatidylserine (PS, negatively charged like SGG) liposomes did not. All of these results suggested the role of human sperm SGG in ZP binding. Mol. Reprod. Dev. 60: 569–578, 2001. © 2001 Wiley‐Liss, Inc.