bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Upper-Troposphere Water Vapor Measurements during AFWEX Using LASE
Authors: Ferrare R. A. , Browell E. V. , Ismail S. , Kooi S. A. , Brasseur L. H. , Brackett V. G. , Clayton M. B. , Barrick J. D. W. , Diskin G. S. , Goldsmith J. E. M. , Lesht B. M. , Podolske J. R. , Sachse G. W. , Schmidlin F. J. , Turner D. D. , Whiteman D. N. , Tobin D. , Miloshevich L. M. , Revercomb H. E. , Demoz B. B. , Di Girolamo P. 
Published: January 2005

AbstractWater vapor mass mixing ratio profiles from NASA's Lidar Atmospheric Sensing Experiment (LASE) system acquired during the Atmospheric Radiation Measurement (ARM)–First International Satellite Cloud Climatology Project (ISCCP) Regional Experiment (FIRE) Water Vapor Experiment (AFWEX) are used as a reference to characterize upper-troposphere water vapor (UTWV) measured by ground-based Raman lidars, radiosondes, and in situ aircraft sensors over the Department of Energy (DOE) ARM Southern Great Plains (SGP) site in northern Oklahoma. LASE was deployed from the NASA DC-8 aircraft and measured water vapor over the ARM SGP Central Facility (CF) site during seven flights between 27 November and 10 December 2000. Initially, the DOE ARM SGP Cloud and Radiation Testbed (CART) Raman lidar (CARL) UTWV profiles were about 5%–7% wetter than LASE in the upper troposphere, and the Vaisala RS80-H radiosonde profiles were about 10% drier than LASE between 8 and 12 km. Scaling the Vaisala water vapor profiles to match the precipitable water vapor (PWV) measured by the ARM SGP microwave radiometer (MWR) did not change these results significantly. By accounting for an overlap correction of the CARL water vapor profiles and by employing schemes designed to correct the Vaisala RS80-H calibration method and account for the time response of the Vaisala RS80-H water vapor sensor, the average differences between the CARL and Vaisala radiosonde upper-troposphere water vapor profiles are reduced to about 5%, which is within the ARM goal of mean differences of less than 10%. The LASE and DC-8 in situ diode laser hygrometer (DLH) UTWV measurements generally agreed to within about 3%–4%. The DC-8 in situ frost point cryogenic hygrometer and Snow White chilled-mirror measurements were drier than the LASE, Raman lidars, and corrected Vaisala RS80H measurements by about 10%–25% and 10%–15%, respectively. Sippican (formerly VIZ Manufacturing) carbon hygristor radiosondes exhibited large variabilities and poor agreement with the other measurements. PWV derived from the LASE profiles agreed to within about 3% on average with PWV derived from the ARM SGP microwave radiometer. The agreement between the LASE and MWR PWV and the LASE and CARL UTWV measurements supports the hypotheses that MWR measurements of the 22-GHz water vapor line can accurately constrain the total water vapor amount and that the CART Raman lidar, when calibrated using the MWR PWV, can provide an accurate, stable reference for characterizing upper-troposphere water vapor.
The Dispersion Relation of Short Wind Waves from Space–Time Wave Measurements*
Authors: Wang David W. , Hwang Paul A. 
Published: January 2005

Abstract
To study the dispersion relation of short wind waves, a linear wave gauge array (WGA) is configured and mounted on a wave-following buoy to conduct in situ space–time measurements of short gravity waves. Results from two field deployments of the WGA buoy in growing seas are presented. The two-dimensional (2D) wavenumber–frequency spectra derived from the space–time measurements provide a direct examination on the relation of wave frequency and wavenumber of short waves in the along-wind direction. Both wavenumber-based and frequency-based phase velocities are extracted from the 2D spectra. The effect of higher harmonics resulting from the Fourier decomposition of nonlinear wave profiles is more prominent to the frequency-based phase velocity than the wavenumber-based phase velocity. The wavenumber-based phase velocity is consistent with that according to the linear dispersion relation, while the frequency-based phase velocity becomes larger due to the higher harmonics.
Minimizing Instrumental Broadening of the Drop Size Distribution with the M-Fast-FSSP
Authors: Schmidt Sebastian , Lehmann Katrin , Wendisch Manfred 
Published: January 2005

Abstract
A modified version of the Fast-FSSP (the so-called M-Fast-FSSP) is introduced. It allows minimization of the instrumental broadening of measured cloud drop size distributions caused by laser beam inhomogeneities. This is achieved by applying a new technique based on a postexperiment stepwise reduction of the probe's sampling volume. For monodisperse glass bead samples it is shown that the width of the measured size distribution is considerably reduced when applying this technique, especially for large glass bead diameters. The instrumental broadening may exceed a factor of about 4 for a mean glass bead diameter of 30 μm. The M-Fast-FSSP was applied in two cloud measurement campaigns. For two specific cloud cases, the profile of the width of the measured drop size distribution changes significantly when applying the method.
Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1–dependent activation of PI-3K/Akt and regulation of Bcl-2 family members
Authors: Limaye Vidya , Li Xiaochun , Hahn Chris , Xia Pu , Berndt Michael C. , Vadas Mathew A. , Gamble Jennifer R. 
Published: January 2005

AbstractSphingosine-1-phosphate (S1P), the bioactive product of sphingosine kinase (SK) activation, is a survival factor for endothelial cells. The mechanism of SK-mediated survival was investigated in endothelial cells with moderately raised intracellular SK activity. Overexpression of SK mediated survival primarily through the activation of the phosphatidyl inositol 3-kinase (PI-3K)/protein kinase B (Akt/PKB) pathway and an associated up-regulation of the antiapoptotic protein B cell lymphoma gene 2 (Bcl-2) and down-regulation of the proapoptotic protein bisindolylmaleimide (Bcl-2 interacting mediator of cell death; Bim). In addition there was an up-regulation and dephosphorylation of the junctional molecule platelet endothelial cell adhesion molecule-1 (PECAM-1), which was obligatory for activation of the PI-3K/Akt pathway, for SK-induced cell survival, and for the changes in the apoptosis-related proteins. Thus, raised intracellular SK activity induced a molecule involved in cell–cell interactions to augment cell survival through a PI-3K/Akt–dependent pathway. This is distinct from the activation of both PI-3K/Akt and mitogen-activated protein kinase (MAPK) pathways seen with exogenously added S1P. Cells overexpressing SK showed enhanced survival under conditions of serum deprivation and absence of attachment to extracellular matrix, suggesting a role for SK in the regulation of vascular phenomena that occur under conditions of stress, such as angiogenesis and survival in unattached states, as would be required for a circulating endothelial cell.
Fibrinogen Philadelphia, a hypodysfibrinogenemia characterized by abnormal polymerization and fibrinogen hypercatabolism due to γ S378P mutation
Authors: Keller Margaret A. , Martinez Josè , Baradet Timothy C. , Nagaswami Chandrasekaran , Chernysh Irina N. , Borowski Meggin K. , Surrey Saul , Weisel John W. 
Published: January 2005

AbstractFibrinogen Philadelphia, a hypodysfibrinogenemia described in a family with a history of bleeding, is characterized by prolonged thrombin time, abnormal fibrin polymerization, and increased catabolism of the abnormal fibrinogen. Turbidity studies of polymerization of purified fibrinogen under different ionic conditions reveal a reduced lag period and lower final turbidity, indicating more rapid initial polymerization and impaired lateral aggregation. Consistent with this, scanning and transmission electron microscopy show fibers with substantially lower average fiber diameters. DNA sequence analysis of the fibrinogen genes A, B, and G revealed a T&gt;C transition in exon 9 resulting in a serine-to-proline substitution near the γ chain C-terminus (S378P). The S378P mutation is associated with fibrinogen Philadelphia in this kindred and was not found in 10 controls. This region of the γ chain is involved in fibrin polymerization, supporting this as the polymerization defect causing the mutation. Thus, this abnormal fibrinogen is characterized by 2 unique features: (1) abnormal polymerization probably due to a major defect in lateral aggregation and (2) hypercatabolism of the mutant protein. The location, nature, and unusual characteristics of this mutation may add to our understanding of fibrinogen protein interactions necessary for normal catabolism and fibrin formation.
Sema4D induces angiogenesis through Met recruitment by Plexin B1
Authors: Conrotto Paolo , Valdembri Donatella , Corso Simona , Serini Guido , Tamagnone Luca , Comoglio Paolo Maria , Bussolino Federico , Giordano Silvia 
Published: January 2005

AbstractSemaphorins, a large family of membrane-bound and secreted proteins, signal through their transmembrane receptors, the plexins. Semaphorins and plexins share structural homologies with scatter factor receptors, a family of tyrosine kinase receptors for which Met is the prototype. Semaphorins have been studied primarily in the developing nervous system, where they act as repelling cues in axon guidance. However, they are widely expressed in several tissues, and their role in epithelial morphogenesis has been recently established. Not much is known about their role in angiogenesis, a key step during embryonic development and adulthood. Here we demonstrate that a semaphorin, Sema4D, is angiogenic in vitro and in vivo and that this effect is mediated by its high-affinity receptor, Plexin B1. Moreover, we prove that biologic effects elicited by Plexin B1 require coupling and activation of the Met tyrosine kinase. In sum, we identify a proangiogenic semaphorin and provide insight about the signaling machinery exploited by Plexin B1 to control angiogenesis.
Prognostic Index for Adult Patients With Acute Myeloid Leukemia in First Relapse
Authors: Breems Dimitri A. , Van Putten Wim L.J. , Huijgens Peter C. , Ossenkoppele Gert J. , Verhoef Gregor E.G. , Verdonck Leo F. , Vellenga Edo , De Greef Georgine E. , Jacky Emanuel , Van der Lelie Johannes , Boogaerts Marc A. , Löwenberg Bob 
Published: January 2005

Purpose The treatment of acute myeloid leukemia (AML) in first relapse is associated with unsatisfactory rates of complete responses that usually are short lived. Therefore, a clinically useful prognostic index can facilitate therapeutic decision making and evaluation of investigational treatment strategies at relapse of AML. Patients and Methods A prognostic score is presented based on the multivariate analysis of 667 AML patients in first relapse among 1,540 newly diagnosed non-M3 AML patients (age 15 to 60 years) entered onto three successive Dutch-Belgian Hemato-Oncology Cooperative Group and the Swiss Group for Clinical Cancer Research Collaborative Group trials. Results Four clinically relevant parameters are included in this index (ie, length of relapse-free interval after first complete remission, cytogenetics at diagnosis, age at relapse, and whether previous stem-cell transplantation was performed). Using this stratification system, three risk groups were defined: a favorable prognostic group A (overall survival [OS] of 70% at 1 year and 46% at 5 years), an intermediate-risk group B (OS of 49% at 1 year and 18% at 5 years), and a poor-risk group C (OS of 16% at 1 year and 4% at 5 years). Conclusion The prognostic index estimates the outcome of AML patients in first relapse using four commonly applied clinical parameters and might identify patients who are candidates for salvage and investigational therapy.
Biosonar performance of foraging beaked whales (Mesoplodon densirostris)
Authors: Madsen P. T. , Johnson M. , de Soto N. Aguilar , Zimmer W. M. X. , Tyack P. 
Published: January 2005

SUMMARYToothed whales (Cetacea, odontoceti) emit sound pulses to probe their surroundings by active echolocation. Non-invasive, acoustic Dtags were placed on deep-diving Blainville's beaked whales (Mesoplodon densirostris) to record their ultrasonic clicks and the returning echoes from prey items, providing a unique view on how a whale operates its biosonar during foraging in the wild. The process of echolocation during prey capture in this species can be divided into search, approach and terminal phases, as in echolocating bats. The approach phase, defined by the onset of detectable echoes recorded on the tag for click sequences terminated by a buzz, has interclick intervals (ICI) of 300-400 ms. These ICIs are more than a magnitude longer than the decreasing two-way travel time to the targets, showing that ICIs are not given by the two-way-travel times plus a fixed, short lag time. During the approach phase, the received echo energy increases by 10.4(±2) dB when the target range is halved, demonstrating that the whales do not employ range-compensating gain control of the transmitter, as has been implicated for some bats and dolphins. The terminal/buzz phase with ICIs of around 10 ms is initiated when one or more targets are within approximately a body length of the whale (2-5 m), so that strong echo returns in the approach phase are traded for rapid updates in the terminal phase. It is suggested that stable ICIs in the search and approach phases facilitate auditory scene analysis in a complex multi-target environment, and that a concomitant low click rate allows the whales to maintain high sound pressure outputs for prey detection and discrimination with a pneumatically driven,bi-modal sound generator.
Body-induced vortical flows: a common mechanism for self-corrective trimming control in boxfishes
Authors: Bartol Ian K. , Gharib Morteza , Webb Paul W. , Weihs Daniel , Gordon Malcolm S. 
Published: January 2005

SUMMARYBoxfishes (Teleostei: Ostraciidae) are marine fishes having rigid carapaces that vary significantly among taxa in their shapes and structural ornamentation. We showed previously that the keels of the carapace of one species of tropical boxfish, the smooth trunkfish, produce leading edge vortices (LEVs) capable of generating self-correcting trimming forces during swimming. In this paper we show that other tropical boxfishes with different carapace shapes have similar capabilities. We conducted a quantitative study of flows around the carapaces of three morphologically distinct boxfishes(spotted boxfish, scrawled cowfish and buffalo trunkfish) using stereolithographic models and three separate but interrelated analytical approaches: digital particle image velocimetry (DPIV), pressure distribution measurements, and force balance measurements. The ventral keels of all three forms produced LEVs that grew in circulation along the bodies, resembling the LEVs produced around delta-winged aircraft. These spiral vortices formed above the keels and increased in circulation as pitch angle became more positive,and formed below the keels and increased in circulation as pitch angle became more negative. Vortices also formed along the eye ridges of all boxfishes. In the spotted boxfish, which is largely trapezoidal in cross section, consistent dorsal vortex growth posterior to the eye ridge was also present. When all three boxfishes were positioned at various yaw angles, regions of strongest concentrated vorticity formed in far-field locations of the carapace compared with near-field areas, and vortex circulation was greatest posterior to the center of mass. In general, regions of localized low pressure correlated well with regions of attached, concentrated vorticity, especially around the ventral keels. Although other features of the carapace also affect flow patterns and pressure distributions in different ways, the integrated effects of the flows were consistent for all forms: they produce trimming self-correcting forces, which we measured directly using the force balance. These data together with previous work on smooth trunkfish indicate that body-induced vortical flows are a common mechanism that is probably significant for trim control in all species of tropical boxfishes.
Non-olfactory chemoreceptors in asymmetric setae activate antennular grooming behavior in the Caribbean spiny lobsterPanulirus argus
Authors: Schmidt Manfred , Derby Charles D. 
Published: January 2005

SUMMARYIn the spiny lobster Panulirus argus the antennules carrying olfactory sensilla called aesthetascs and several types of other non-olfactory sensilla accompanying them are frequently groomed by the third maxillipeds in a stereotyped behavioral pattern. This behavior can be elicited by chemical stimulation with l-glutamate. Using selective sensillar ablations,we tested whether this behavior is driven by the numerous aesthetascs, which have been implicated as mediating this chemically elicited antennular grooming behavior in a previous investigation, or other, less numerous sensilla called asymmetric setae, which are tightly associated with aesthetascs. The selective sensilla ablations showed that the asymmetric setae are necessary and sufficient for driving chemically elicited antennular grooming. Bilateral elimination of the ca. 160 asymmetric setae almost completely abolished the behavior, whereas bilateral elimination of the ca. 2600 aesthetascs or of another type of sensilla associated with them (guard setae) did not cause a reduction in chemically elicited antennular grooming. Microscopical analysis of the morphological properties of the asymmetric setae revealed the presence of a terminal pore at the tip of the seta and a phalloidin-positive scolopale below its base. Since these structures have been identified in decapod crustaceans as modality-specific structures of bimodal chemo- and mechanosensory sensilla, we conclude that the asymmetric setae belong to this type of sensilla and thus have the appropriate features to function as chemoreceptors in the elicitation of antennular grooming. The identification of asymmetric setae and not aesthetascs as the drivers of chemically elicited antennular grooming suggests that it is not the olfactory pathway in the brain but a parallel pathway, constituted mainly by the lateral antennular neuropils, that is the neuronal substrate of this behavior. The lateral antennular neuropils receive non-olfactory sensory input from the antennule and contain the major arborizations of antennular motoneurons, allowing that direct sensory-motor coupling is involved in mediating the chemical elicitation of antennular grooming behavior.
Dynamic pressure maps for wings and tails of pigeons in slow, flapping flight, and their energetic implications
Authors: Usherwood James R. , Hedrick Tyson L. , McGowan Craig P. , Biewener Andrew A. 
Published: January 2005

SUMMARYDifferential pressure measurements offer a new approach for studying the aerodynamics of bird flight. Measurements from differential pressure sensors are combined to form a dynamic pressure map for eight sites along and across the wings, and for two sites across the tail, of pigeons flying between two perches. The confounding influence of acceleration on the pressure signals is shown to be small for both wings and tail. The mean differential pressure for the tail during steady, level flight was 25.6 Pa, which, given an angle of attack for the tail of 47.6°, suggests the tail contributes 7.91% of the force required for weight support, and requires a muscle-mass specific power of 19.3 W kg-1 for flight to overcome its drag at 4.46 m s-1. Differential pressures during downstroke increase along the wing length, to 300-400 Pa during take-off and landing for distal sites. Taking the signals obtained from five sensors sited along the wing at feather bases as representative of the mean pressure for five spanwise elements at each point in time, and assuming aerodynamic forces act within the x-z plane (i.e. no forces in the direction of travel) and perpendicular to the wing during downstroke, we calculate that 74.5% of the force required to support weight was provided by the wings, and that the aerodynamic muscle-mass specific power required to flap the wings was 272.7 W kg-1.
Electrical activity of caudal neurosecretory neurons in seawater and freshwater-adapted Platichthys flesus, in vivo
Authors: Ashworth A. J. , Banks J. R. , Brierley M. J. , Balment R. J. , McCrohan C. R. 
Published: January 2005

SUMMARY
This study examined the electrical firing activity of neuroendocrine Dahlgren cells in the caudal neurosecretory system (CNSS) of the euryhaline flounder in vivo. Intracellular recordings revealed generally similar activity patterns and membrane properties to those previously reported in vitro. To investigate the potential role of the CNSS in osmoregulatory adaptation, extracellular, multiunit, recordings compared the activity patterns of Dahlgren cells in fully seawater- and freshwater-adapted fish. The proportion of cells showing bursting (as opposed to phasic or tonic) activity was greater in seawater-than in freshwater-adapted fish, as was the Correlation Index, a measure of the degree of correlation between firing activities of cells recorded simultaneously from the same preparation. Acute transfer of fish from seawater to freshwater gill perfusion led to recruitment of previously silent Dahlgren cells and a reduction in Correlation Index;freshwater to seawater transfer increased the Correlation Index. Severing the spinal cord anterior to the CNSS led to an increase in overall Dahlgren cell activity. Electrical stimulation of branchial nerve branches providing input to the brainstem, or tactile (pinch) stimulation of lips or fins, led to a reduction in CNSS activity lasting up to 500 s, indicating the presence of descending modulatory pathways from the brain. These results are consistent with a role for CNSS neuropeptides, urotensins, in supporting survival in a hypertonic, seawater, environment.
Troponin T expression in trout red muscle correlates with muscle activation
Authors: Coughlin David J. , Caputo Nicholas D. , Bohnert Krista L. , Weaver Frances E. 
Published: January 2005

SUMMARYRed or aerobic muscle from the anterior of rainbow trout Oncorhynchus mykiss activates (generates force) more quickly than that from the posterior. TnT is a component of the troponin complex that modulates muscle activation once Ca2+ is bound. Since trout express at least two forms of TnT in their red muscle (S1 and S2), the differential expression of these two forms was predicted to explain variations in contractile properties. TnT isoforms from trout muscle were identified through hydroxy-apatite chromatography of purified myofibrillar proteins followed by SDS-PAGE. Western blots employing a mammalian anti-troponin T monoclonal antibody were used to identify TnT isoforms. The relative expression of the two isoforms of TnT was then examined at seven longitudinal positions from each of three fish using SDS-PAGE and densitometry on the silver-stained TnT bands. A significant shift in expression was detected from anterior to posterior in all three fish with TnT S1 becoming more dominant in the posterior red muscle. As predicted, a shift in TnT expression was associated with the decrease in activation rate along the length of the fish. This study was then extended to include a different species of salmonid, brook trout Salvelinus fontinalis, to explore the generality of TnT modulation of muscle activation. Muscle contractile properties were determined from anterior and posterior muscle, and relative expression of S1 and S2 was determined. Unlike rainbow trout, there is no consistent longitudinal pattern of muscle activation in brook trout:some fish have kinetically faster muscle in the anterior, some in the posterior. Similarly, there is no consistent pattern of TnT expression. Individual analysis of muscle activation and TnT expression in brook trout provides insight into the role of TnT in modulating muscle activation in slow fish muscle.