bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of alginate macrocapsule device for long-term normoglycaemia in the treatment of type 1 diabetes mellitus with pancreatic cell sheet engineering
Authors: Duman Büşra Öncel , Yazir Yusufhan , Halbutoğullari Zehra Seda , Mert Serap , Öztürk Ahmet , Gacar Gülçin , Duruksu Gökhan 
Published: January 2024

Abstract
Type 1 diabetes-mellitus (T1DM) is characterized by damage of beta cells in pancreatic islets. Cell-sheet engineering, one of the newest therapeutic approaches, has also been used to create functional islet systems by creating islet/beta cell-sheets and transferring these systems to areas that require minimally invasive intervention, such as extrahepatic areas. Since islets, beta cells, and pancreas transplants are allogeneic, immune problems such as tissue rejection occur after treatment, and patients become insulin dependent again. In this study, we aimed to design the most suitable cell-sheet treatment method and macrocapsule-device that could provide long-term normoglycemia in rats. Firstly, mesenchymal stem cells (MSCs) and beta cells were co-cultured in a temperature-responsive culture dish to obtain a cell-sheet and then the cell-sheets macroencapsulated using different concentrations of alginate. The mechanical properties and pore sizes of the macrocapsule-device were characterized. The viability and activity of cell-sheets in the macrocapsule were evaluated in vitro and in vivo. Fasting blood glucose levels, body weight, and serum insulin & C-peptide levels were evaluated after transplantation in diabetic-rats. After the transplantation, the blood glucose level at 225 mg dl–1 on the 10th day dropped to 168 mg dl–1 on the 15th day, and remained at the normoglycemic level for 210 days. In this study, an alginate macrocapsule-device was successfully developed to protect cell-sheets from immune attacks after transplantation. The results of our study provide the basis for future animal and human studies in which this method can be used to provide long-term cellular therapy in T1DM patients.
Effect of TIG welding parameters on 316 L stainless steel joints using taguchi L27 approach
Authors: Khrais Samir , Mohammed Awsan , Al Ahmad Abdel , Darabseh Tariq 
Published: January 2024

Abstract
The AISI 316 L stainless steel was welded using Tungsten Inert Gas (TIG) welding, utilizing ternary shielding gases Argon (Ar), Helium (He), and Nitrogen (N2). This study aimed to assess the effects of these ternary shielding gases on the microstructure, bead profile, and bead appearance. It provides a comprehensive grasp of welding parameters’ interplay with shielding gas compositions, enabling engineers to make informed choices that significantly influence the excellence, productivity, and lastingness of the welding process. The Taguchi L-27 approach was employed, incorporating different contents of N2 (2.5 vol. % to 7.5 vol. %) and He (10 vol. % to 30 vol. %) within the Ar shielding gas composition. Additionally, welding current intensities, ranging from 120 A to 180 A, were also used in the experiment. The results demonstrated that higher content of He and N2 resulted in elevated levels of austenite-forming elements. Therefore, for TIG welding at the arc current intensity of 150 A, it is recommended to utilize the shielding gas mixtures (2.5 vol. % N2 + 10 vol. % He + 87.5 vol. % Ar). Furthermore, by augmenting the content of both N2 and He within the Ar shielding gas mixture, in addition to adjusting the arc current, a notable expansion in both the width and depth of the weld profile was achieved. This achievement, in turn, played a pivotal role in securing comprehensive fusion throughout the welding process.
Fully solution-processed n-i-p type perovskite solar cells with efficiency over 19% enabled by a hydrophobic PEDOT:F interlayer and silver nanowire top electrode
Authors: Zhao Zhenhua , Liu Yang , Ding Changzeng , Zha Wusong , Luo Qun , Zhou Yinhua , Ma Chang-Qi 
Published: January 2024

Abstract
Solution-processed top electrode is the key technology for the realization of fully solution-processed perovskite solar cells (PSCs). In this paper, we reported the preparation of fully-solution processed n-i-p type PSCs with spray-coated silver nanowires (AgNWs) top electrode. Experiment results demonstrated that low power conversion efficiency (PCE) of 16.00% with a low fill factor of 67.41% was obtained for the reference cell, when the AgNWs is directly coated on the 2,2′,7,7′-tetrakis(N,N-di-p-methoxyphenylamine)-9,9′-spirobifluorene (Spiro-OMeTAD) layer. This result indicates a charge extraction barrier at the Spiro-OMeTAD/AgNWs interface, which could be attributed to the poor compatibility between Spiro-OMeTAD and AgNWs layers. By inserting a thin layer of poly(3,4-ethylenedioxythiophene):perfluorinated sulfonic acid ionomers (PEDOT:F), a modified PEDOT with lower surface energy, the interface connection between Spiro-OMeTAD/PEDOT:F and AgNWs was improved, leading to an enhanced PCE of up to 19.11%. In addition, the PEDOT:F-based cells retained 64% of the initial PCE after operating at the maximum power point for 1800 h, whereas the reference cells retained only 30% of the initial PCE. The current work proved that PEDOT:F/AgNWs could be an excellent option for fully-solution processed n-i-p type PSCs.
Indoor air quality monitoring and source apportionment using low-cost sensors
Authors: Higgins Christina , Kumar Prashant , Morawska Lidia 
Published: January 2024

Abstract
Understanding of the various sources of indoor air pollution requires indoor air quality (IAQ) data that is usually lacking. Such data can be obtained using unobtrusive, low-cost sensors (LCS). The aim of this review is to examine the recent literature published on LCS for IAQ measurements and to determine whether these studies employed any methods to identify or quantify sources of indoor air pollution. Studies were reviewed in terms of whether any methods of source apportionment were employed, as well as the microenvironment type, geographical location, and several metrics relating to the contribution of outdoor pollutant ingress versus potential indoor pollutant sources. We found that out of 60 relevant studies, just four employed methods for source apportionment, all of which utilised receptor models. Most studies were undertaken in residential or educational environments. There is a lack of data on IAQ in other types of microenvironments and in locations outside of Europe and North America. There are inherent limitations with LCS in terms of producing data which can be utilised in source apportionment models. This applies to external pollution data, however IAQ can be even more challenging to measure due to its characteristics. The indoor environment is heterogeneous, with significant variability within the space as well as between different microenvironments and locations. Sensor placement, occupancy, and activity reports, as well as measurements in different microenvironments and locations, can contribute to understanding this variability. Outdoor pollutants can ingress into the space via the building envelope, however measurement of external pollution and environmental conditions, as well as recording details on the building fabric and ventilation conditions, can help apportion external contributions. Whether or not source apportionment models are employed on indoor data from LCS, there are parameters which, if carefully considered during measurement campaigns, can aid in source identification of pollutants.
Towards truly sustainable IoT systems: the SUPERIOT project
Authors: Katz Marcos , Paso Tuomas , Mikhaylov Konstantin , Pessoa Luis , Fontes Helder , Hakola Liisa , Leppäniemi Jaakko , Carlos Emanuel , Dolmans Guido , Rufo Julio , Drzewiecki Marcin , Sallouha Hazem , Napier Bruce , Branquinho André , Eder Kerstin 
Published: January 2024

Abstract
This paper provides an overview of the SUPERIOT project, an EU SNS JU (Smart Networks and Services Joint Undertaking) initiative focused on developing truly sustainable IoT systems. The SUPERIOT concept is based on a unique holistic approach to sustainability, proactively developing sustainable solutions considering the design, implementation, usage and disposal/reuse stages. The concept exploits radio and optical technologies to provide dual-mode wireless connectivity and dual-mode energy harvesting as well as dual-mode IoT node positioning. The implementation of the IoT nodes or devices will maximize the use of sustainable printed electronics technologies, including printed components, conductive inks and substrates. The paper describes the SUPERIOT concept, covering the key technical approaches to be used, promising scenarios and applications, project goals and demonstrators which will be developed to the proof-of-concept stage. In addition, the paper briefly discusses some important visions on how this technology may be further developed in the future.
Antibiofilm approaches as a new paradigm for treating infections
Authors: Reffuveille Fany , Dghoughi Yasser , Colin Marius , Torres Marcelo D T , de la Fuente-Nunez Cesar 
Published: January 2024

Abstract
The lack of effective antibiotics for drug-resistant infections has led the World Health Organization to declare antibiotic resistance a global priority. Most bacterial infections are caused by microbes growing in structured communities called biofilms. Bacteria growing in biofilms are less susceptible to antibiotics than their planktonic counterparts. Despite their significant clinical implications, bacterial biofilms have not received the attention they warrant, with no approved antibiotics specifically designed for their eradication. In this paper, we aim to shed light on recent advancements in antibiofilm strategies that offer compelling alternatives to traditional antibiotics. Additionally, we will briefly explore the potential synergy between computational approaches, including the emerging field of artificial intelligence, and the accelerated design and discovery of novel antibiofilm molecules in the years ahead.
A railway fastener inspection method based on lightweight network
Authors: Wang Yue , Wang Ji , Zheng Shubin , Li Liming , Xie Xing 
Published: January 2024

Abstract
To ensure the safety of train operations, regular inspection and maintenance of railway fastener is crucial. Currently, computer vision-based fastener inspection has become the primary method. However, to improve detection accuracy, model complexity has been continuously increasing, leading to a decrease in detection efficiency and seriously affecting real-time fastener inspection task. To address this issue, we propose a fastener inspection method based on lightweight network. First, we introduce YOLOv5n-Faster, where this model selects YOLOv5n as the base network for fastener localization and uses partial convolution (PConv) from FasterNet for lightweight design, while using CoordConv to improve localization accuracy, thereby achieving efficient fastener localization task. Then, we perform lightweight design on the overall network structure and Block module of the ConvNeXt-T to propose the fastener state inspection model, Light ConvNeXt, for rapid fastener classification. Experimental results show that our proposed fastener localization model achieves a mean average precision (mAP) of 84.7%, a detection speed of 161 FPS. The fastener state inspection model achieves an accuracy of 99.7%.
Optimal fuel supply of green ammonia to decarbonise global shipping
Authors: Verschuur Jasper , Salmon Nicholas , Hall Jim , Bañares-Alcántara René 
Published: January 2024

Abstract
Green ammonia has been proposed as a technologically viable solution to decarbonise global shipping, yet there are conflicting ambitions for where global production, transport and fuelling infrastructure will be located. Here, we develop a spatial modelling framework to quantify the cost-optimal fuel supply to decarbonise shipping in 2050 using green ammonia. We find that the demand for green ammonia by 2050 could be three to four times the current (grey) ammonia production, requiring major new investments in infrastructure. Our model predicts a regionalisation of supply, entailing a few large supply clusters that will serve regional demand centres, with limited long-distance shipping of green ammonia fuel. In this cost-efficient model, practically all green ammonia production is predicted to lie within 40° latitudes North/South. To facilitate this transformation, investments worth USD 2 trillion would be needed, half of which will be required in low- and middle-income countries.
Long-term predictors of developmental outcome and disease burden in SCN1A-positive Dravet syndrome
Authors: Feng Tony , Makiello Phoebe , Dunwoody Benjamin , Steckler Felix , Symonds Joseph D , Zuberi Sameer M , Dorris Liam , Brunklaus Andreas 
Published: January 2024

Abstract
Dravet syndrome is a severe infantile onset developmental and epileptic encephalopathy associated with mutations in the sodium channel alpha 1 subunit gene SCN1A. Prospective data on long-term developmental and clinical outcomes are limited; this study seeks to evaluate the clinical course of Dravet syndrome over a 10-year period and identify predictors of developmental outcome. SCN1A mutation-positive Dravet syndrome patients were prospectively followed up in the UK from 2010 to 2020. Caregivers completed structured questionnaires on clinical features and disease burden; the Epilepsy & Learning Disability Quality of Life Questionnaire, the Adaptive Behavioural Assessment System-3 and the Sleep Disturbance Scale for Children. Sixty-eight of 113 caregivers (60%) returned posted questionnaires. Developmental outcome worsened at follow-up (4.45 [SD 0.65], profound cognitive impairment) compared to baseline (2.9 [SD 1.1], moderate cognitive impairment, P &lt; 0.001), whereas epilepsy severity appeared less severe at 10-year follow-up (P = 0.042). Comorbidities were more apparent at 10-year outcome including an increase in autistic features (77% [48/62] versus 30% [17/57], χ2 = 19.9, P &lt; 0.001), behavioural problems (81% [46/57] versus 38% [23/60], χ2 = 14.1, P &lt; 0.001) and motor/mobility problems (80% [51/64] versus 41% [24/59], χ2 = 16.9, P &lt; 0.001). Subgroup analysis demonstrated a more significant rise in comorbidities in younger compared to older patients. Predictors of worse long-term developmental outcome included poorer baseline language ability (P &lt; 0.001), more severe baseline epilepsy severity (P = 0.003) and a worse SCN1A genetic score (P = 0.027). Sudden unexpected death in epilepsy had not been discussed with a medical professional in 35% (24/68) of participants. Over 90% of caregivers reported a negative impact on their own health and career opportunities. Our study identifies important predictors and potential biomarkers of developmental outcome in Dravet syndrome and emphasizes the significant caregiver burden of illness. The negative impact of epilepsy severity at baseline on long-term developmental outcomes highlights the importance of implementing early and focused therapies whilst the potential impact of newer anti-seizure medications requires further study.
Annular remodelling predicts outcome in isolated severe tricuspid regurgitation: a registry-based echocardiographic analysis
Authors: Arfsten Henrike , König Andreas , Geller Welf , Bodner Lorenz , Dannenberg Varius , Prausmüller Suriya , Bartko Philipp E , Binder Thomas , Hengstenberg Christian , Goliasch Georg , Schneider-Reigbert Matthias 
Published: January 2024

Abstract

Aims
Depending on volume status, secondary tricuspid regurgitation (sTR) has a strong dynamic component. In contrast, associated structural dilatation of the tricuspid annulus and the right heart chambers may be less volume dependent. This study aimed to assess the prognostic value of right heart remodelling in isolated severe sTR (isoTR).

Methods and results
A total of 36 000 patients from the longitudinal echocardiographic database of our tertiary centre were screened for severe isoTR [vena contracta (VC) ≥ 7 mm] in the absence of atrial fibrillation (AF), other valve disease, and/or reduced systolic left ventricular function. Echocardiographic examinations were re-read, focusing on right ventricular (RV) parameters and on quantitative and qualitative parameters of isoTR. All-cause mortality was defined as the primary endpoint. Two hundred and sixteen patients fulfilled the inclusion criteria. Severe TR was predominant; only few were classified in the new grades massive [n = 23 (10%)] and torrential TR [n = 4 (2%)]. During a median follow-up of 35 months (20–53), all-cause mortality was 31% (n = 67). Multivariate Cox regression analysis revealed no association of VC, effective regurgitant orifice area, or regurgitant volume with all-cause mortality. However, indexed RV end-diastolic diameter (P &lt; 0.001), indexed right atrial dimensions (P = 0.019), and particularly tricuspid valve (TV) annulus diameter diastole index (P = 0.002) and TV annulus diameter systole index (P = 0.001) were significantly associated with outcome.

Conclusion
Severe isolated TR in the absence of AF is a rare finding with a grim prognosis. Tricuspid annular diameter dimensions rather than quantitative measures of TR proved to be of significant prognostic value indicating a continuous remodelling leading to a ‘point of no return’ with a dismal outcome.
The Association of Skeletal Muscle Energetics With Recurrent Falls in Older Adults Within the Study of Muscle, Mobility and Aging
Authors: Kramer Philip A , Zamora Ezequiel , Barnes Haley N , Strotmeyer Elsa S , Glynn Nancy W , Lane Nancy E , Coen Paul M , Cawthon Peggy M , Goodpaster Bret H , Newman Anne B , Kritchevsky Stephen B , Cummings Steven R 
Published: January 2024

Abstract

Background
Falls in the older population are a major public health concern. While many physiological and environmental factors have been associated with fall risk, muscle mitochondrial energetics has not yet been investigated.

Methods
In this analysis, 835 Study of Muscle, Mobility and Aging (SOMMA) participants aged 70–94 were surveyed for number of falls (total), recurrent falls (2+), and fall-related injuries over the past 12 months at baseline and again after 1 year. Skeletal muscle energetics were assessed at baseline in vivo using 31P Magnetic Resonance Spectroscopy for the maximal rate of adenosine triphosphate recovery (ATPmax) after an acute bout of exercise, and ex vivo by High-Resolution Respirometry for the maximal rate of complex I and II supported oxygen consumption (MaxOXPHOS) in permeabilized muscle fibers from the vastus lateralis.

Results
At least 1 fall was reported in 28.7% of SOMMA participants in the first year of the study, with 12% of older adults reporting recurrent falls (2+). Individuals who experienced recurrent falls had a slower 400-m walk gait speed (1.0 ± 0.2 vs 1.1 ± 0.2, p &lt; .001), reported fewer alcoholic drinks per week in the past year (2.4 ± 4.3 vs 2.8 ± 4.4, p = .054), and took a significantly greater number of medication in the 30 days before their baseline visit (5.6 ± 4.4 vs 4.2 ± 3.4, p &lt; .05). A history of falls was reported in 63% of individuals who experienced recurrent falls in the first year of the study compared to 22.8% who experienced 1 or fewer falls. MaxOXPHOS was significantly lower in those who reported recurrent falls (p = .008) compared to those with 1 or fewer falls, but there was no significant difference in ATPmax (p = .369). Neither muscle energetics measure was significantly associated with total number of falls or injurious falls, but recurrent falls were significantly higher with lower MaxOXPHOS (risk ratio = 1.33, 95% confidence interval = 1.02–1.73, p = .033). However, covariates accounted for the increased risk.

Conclusions
Mitochondrial energetics were largely unrelated to fall risk in older adults when accounting for variables, suggesting that the complex etiology of falls may not be related to a single “hallmark of aging” biological pathway.
Relationship between physical activity and locomotive syndrome among young and middle-aged Japanese workers
Authors: Watanabe Kazuhiko , Lu Xi , Masuda Shota , Miyamoto Takeshi , Katoh Takahiko 
Published: January 2024

Abstract
Objectives: This study aimed to examine the relationship between physical activity (PA) and locomotive syndrome (LS) among young and middle-aged Japanese workers.
Methods: This cross-sectional study included 335 participants from a company in Kumamoto, Japan. LS was evaluated using the 25-question Geriatric Locomotive Function Scale (GLFS-25); a GLFS-25 score ≥7 was defined as LS. Weekly PA was measured using the International Physical Activity Questionnaire. Work-related PA (time spent sitting, standing, walking, and strenuous work per day) and sedentary breaks were measured using a Work-related Physical Activity Questionnaire. Screen usage (television [TV], smartphones, tablets, and personal computers) during leisure time was recorded. The association between PA and LS was examined using a multivariate logistic regression analysis adjusted for age, sex, body mass index, history of musculoskeletal disorders, cancer, stroke, occupation, employment type, work time, shift system, employment status, and body pain.
Results: A total of 149 participants had LS. Fewer sedentary breaks during work (&gt;70-minute intervals, odds ratio [OR] = 2.96; prolonged sitting, OR = 4.12) and longer TV viewing time (≥180 minutes, OR = 3.02) were significantly associated with LS. In contrast, moderate PA (OR = 0.75) was significantly associated with a lower risk of LS.
Conclusions: Fewer sedentary breaks during work and longer TV viewing time could increase the risk of LS in young and middle-aged Japanese workers.
Codelivery of TGFβ and Cox2 siRNA inhibits HCC by promoting T-cell penetration into the tumor and improves response to Immune Checkpoint Inhibitors
Authors: Kim Wookhyun , Ye Zhou , Simonenko Vera , Shahi Aashirwad , Malikzay Asra , Long Steven Z , Xu John J , Lu Alan , Horng Jau-Hau , Wu Chang-Ru , Chen Pei-Jer , Lu Patrick Y , Evans David M 
Published: January 2024

Abstract
Upregulation of TGFβ and Cox2 in the tumor microenvironment results in blockade of T-cell penetration into the tumor. Without access to tumor antigens, the T-cell response will not benefit from administration of the immune checkpoint antibodies. We created an intravenous polypeptide nanoparticle that can deliver two siRNAs (silencing TGFβ and Cox2). Systemic administration in mice, bearing a syngeneic orthotopic hepatocellular carcinoma (HCC), delivers the siRNAs to various cells in the liver, and significantly reduces the tumor. At 2 mg/kg (BIW) the nanoparticle demonstrated a single agent action and induced tumor growth inhibition to undetectable levels after five doses. Reducing the siRNAs to 1mg/kg BIW demonstrated greater inhibition in the presence of PD-L1 mAbs. After only three doses BIW, we could still recover a smaller tumor and, in tumor sections, showed an increase in penetration of CD4+ and CD8+ T-cells deeper into the remaining tumor that was not evident in animals treated with non-silencing siRNA. The combination of TGFβ and Cox2 siRNA co-administered in a polypeptide nanoparticle can act as a novel therapeutic alone against HCC and may augment the activity of the immune checkpoint antibodies. Silencing TGFβ and Cox2 converts an immune excluded (cold) tumor into a T-cell inflamed (hot) tumor.