bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro effect of light‐cure dental adhesive on IL‐6 release from LPS‐stimulated and unstimulated macrophages
Authors: Kostoryz Elisabet L. , Eick J. David , Chappelow Cecil C. , Glaros Alan G. , Wetmore Lori , Yourtee David M. 
Published: March 2003

AbstractThe objective of this study was to measure IL‐6 release from LPS‐stimulated and ‐unstimulated macrophages exposed to extracts from fresh and aged Scotchbond Multipurpose Plus™ adhesive disks (5 mm in diameter by 2 mm in thickness) light cured for 10, 20, or 40 s. One set of disks was aged for 16 weeks at 4°C. Extracts were prepared by incubating three disks in 1 mL of serum‐free culture medium for 72 h at 37°C. Then macrophages (RAW 264.7) were exposed to the extracts (6.25–50 μL) for 72 h at 37°C/5% CO2. Supernatants were analyzed for cytokine levels (ELISA), and the monolayer of cells was assessed for viability (MTT assay). Unlike adhesive disk age, curing time affected cell viability. Disk extracts cured for 10 s were more cytotoxic (p < 0.05) than were extracts from 20‐ or 40‐s cured disks. Macrophage release of IL‐6 was stimulated significantly (p < 0.01) by extracts from fresh 10‐s cured disks, up to 777 pg/mL and by 2 μg/mL of LPS (1174 pg/mL). The LPS response was significantly (p < 0.05) suppressed by 50 μL of extracts, which may be related to the enhanced cytotoxicity exhibited by LPS in combination with extracts. This study has demonstrated the possibility that IL‐6 release is stimulated by light‐cure dental adhesive applications using 10‐s curings. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 89–94, 2003
Surface conditions of Nitinol wires, tubing, and as‐cast alloys. The effect of chemical etching, aging in boiling water, and heat treatment
Authors: Shabalovskaya S. A. , Anderegg J. , Laab F. , Thiel P. A. , Rondelli G. 
Published: March 2003

AbstractThe surface conditions of Nitinol wires and tubing were evaluated with the use of X‐ray photoelectron spectroscopy, high‐resolution Auger spectroscopy, electron backscattering, and scanning‐electron microscopy. Samples were studied in the as‐received state as well as after chemical etching, aging in boiling water, and heat treatment, and compared to a mechanically polished 600‐grit‐finish Nitinol surface treated similarly. General regularities in surface behavior induced by the examined surface treatments are similar for wires, tubing, and studied as‐cast alloy, though certain differences in surface Ni concentration were observed. Nitinol wires and tubing from various suppliers demonstrated great variability in Ni surface concentration (0.5–15 at.%) and Ti/Ni ratio (0.4–35). The wires in the as‐received state, with the exception of those with a black oxide originating in the processing procedure, revealed nickel and titanium on the surface in both elemental and oxidized states, indicating a nonpassive surface. Shape‐setting heat treatment at 500 °C for 15 min resulted in tremendous increase in the surface Ni concentration and complete Ni oxidation. Preliminary chemical etching and boiling in water successfully prevented surface enrichment in Ni, initially resulting from heat treatment. A stoichiometric uniformly amorphous TiO2 oxide generated during chemical etching and aging in boiling water was reconstructed at 700 °C, revealing rutile structure. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 65B: 193–203, 2003
Cell proliferation and differentiation during fracture healing are influenced by locally applied IGF‐I and TGF‐β1: Comparison of two proliferation markers, PCNA and BrdU
Authors: Wildemann B. , Schmidmaier G. , Ordel S. , Stange R. , Haas N.P. , Raschke M. 
Published: March 2003

AbstractGrowth factors IGF‐I and TGF‐β1 are known to stimulate fracture healing. The purpose of this study was to investigate the role of locally applied IGF‐I and TGF‐β1 during the early phase of fracture healing (Days 5, 10, and 15 after fracture) on cellular processes like proliferation and differentiation in a rat model. Two different immunohistochemical markers were used to analyze cell proliferation: (1) injection of the thymidine analogue BrdU and subsequent immunohistochemical staining for BrdU‐positive nuclei, and (2) the antibody against the “proliferating cell nuclear antigen” (PCNA). In comparison, both methods revealed similar results concerning the types of proliferating cells at the different time points and the two groups. Labeling indices of both methods showed very good correlation (e.g., rs: 0.887 and p < 0.001 at day 10 in the control group without growth factors). Comparison of the callus morphology and the proliferation rate showed differences during fracture healing due to the local application of IGF‐I and TGF‐β1 from coated implants. At Day 5 the callus of the group treated with growth factors displayed an earlier appearance of cartilage compared to the control group. This was accompanied by an onset of cell proliferation in chondrocytes. Likewise, at the later time points an enhanced maturation of the callus tissue and the proliferation pattern were detectable in the growth‐factor group. These results indicate that local application of IGF‐I and TGF‐β1 accelerates early cellular processes during fracture healing. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 65B: 150–156, 2003
IGF‐I and TGF‐Beta 1 incorporated in a poly(D,L‐lactide) implant coating stimulates osteoblast differentiation and collagen‐1 production but reduces osteoblast proliferation in cell culture
Authors: Schmidmaier G. , Wildemann B. , Lübberstedt M. , Haas N.P. , Raschke M. 
Published: March 2003

AbstractPrevious in vivo studies revealed a stimulating effect of locally applied IGF‐I and TGF‐β1 released from poly(D,L‐lactide)‐coated titanium implants on rat and porcine fracture healing. The purpose of the present study was to evaluate the effect of IGF‐I (5% w/w) and TGF‐β1 (1% w/w) and the carrier PDLLA on osteoblasts in cell culture to improve the understanding of these growth factors. The well‐characterized human osteoblast cell line hFOB 1.19 was used in the study. The implants and cells were cocultured in a noncontact manner. The cells were incubated for 10 days in total, and the implants (n = 6 each group and time point) were added for 1 h, 12 h, 24 h, 2 d, 4 d, or 10 d. To analyze a possible effect of the growth factors or the coating, cell proliferation, metabolism, and differentiation were investigated. As an indicator for differentiation the production of collagen I was chosen. All experimental groups showed comparable cell vitality. No change in the pH of the medium was detectable between the analyzed groups. When the effect of the titanium implant and the PDLLA coating were compared with the control culture, no differences in proliferation, metabolic activity, and collagen I production were detectable. The osteoblasts treated with IGF‐I and TGF‐β1 released from PDLLA revealed a significantly enhanced collagen I production with a decrease in proliferation and metabolic activity compared to the other groups. No significant differences in collagen I production were seen due to the incubation time points. None of the experimental groups evoked an immunological response on mouse macrophages. In conclusion, the PDLLA‐carrier showed no negative effect on osteoblasts, whereas the incorporated growth factors stimulated osteoblast differentiation. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 65B: 157–162, 2003
Properties and performance of sulfide‐substituted plasticized poly(vinyl chloride) as a biomaterial
Authors: Lakshmi S. , Jayakrishnan A. 
Published: March 2003

AbstractPlasticized poly(vinyl chloride) (PVC) was surface modified by nucleophilic substitution of the chlorine atoms of PVC by sulfide ions in aqueous media in the presence of a phase‐transfer catalyst (PTC) to make it migration resistant. The modified PVC was sterilized by steam autoclaving and gamma radiation and subjected to plasticizer extraction in an extraction medium such as hexane. Virtually no plasticizer migrated out of modified PVC into the medium even after prolonged incubation for 30 d. The properties of the modified surface were evaluated by contact angle measurements, scanning electron microscopy (SEM), cell‐culture studies, hemolysis assay, and whole‐blood clotting time measurements. The mechanical properties of modified sheets were evaluated by measurements of tensile strength and strain at break after subjecting the specimens to different modes of sterilization. The modified PVC surface showed enhanced wettability and was found to be non‐hemolytic and non‐cytotoxic. Whole‐blood clotting profiles of unmodified and modified PVC were virtually the same. Modification imparted slight yellow color to the material. Surface modification resulted in an 8% decrease in the ultimate stress and 28% decrease in the strain at break values. The sulfide‐substituted PVC was found to be blood compatible and would be useful in applications such as tubing for transfusion, dialysis etc., where migration resistance is important. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 65B: 204–210, 2003
A quantitative structure–activity relationship analysis on a series of alkyl benzenes metabolized by human cytochrome P450 2E1
Authors: Lewis David F. V. , Sams Craig , Loizou George D. 
Published: March 2003

AbstractThe results of quantitative structure–activity relationships for eight alkyl benzenes undergoing oxidative metabolism via human CYP2E1 are reported. Molecular orbital calculations via the AM1 method were employed for the generation of electronic structural descriptors against experimentally generated kinetic data for CYP2E1‐mediated metabolism.The findings point to the importance of electronic structural properties of the molecules themselves, particularly the role of frontier orbitals, in determining rates of metabolism. Other factors appear to be responsible for the affinity of these substrates for the CYP2E1 enzyme however, such as its lipophilic character.The results are consistent with the interactive molecular modeling of these compounds within the putative active site of human CYP2E1 constructed from the CYP2C5 template, where it was found that π–π stacking interactions between aromatic rings are important for the binding of substrates to the CYP2E1 active site, together with contributions from desolvation entropy changes accompanying substrate binding. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:47–52, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10055
Isolation, sequencing, and expression of a cDNA for the HXM‐A form of xenobiotic/medium‐chain fatty acid:CoA ligase from human liver mitochondria
Authors: Vessey Donald A. , Lau Eva , Kelley Michael , Warren Robert S. 
Published: March 2003

AbstractThe purification of xenobiotic/medium‐chain fatty acid:CoA ligases (XM‐ligases) from human liver mitochondria resulted in the isolation of two chromatographically separable forms (HXM‐A and HXM‐B). These two forms were purified to near homogeneity, cleaved with cyanogen bromide, the resulting peptides separated, and the N‐terminus of two of the peptides partially sequenced. Identical sequences were obtained for HXM‐A and HXM‐B for the two peptides. These sequences were used to design probes for screening a human liver cDNA library. This resulted in the isolation of two overlapping cDNAs. Using these sequences we were able to design PCR primers that resulted in the isolation of a full‐length cDNA from a human cDNA library. The cDNA contained 1731 bp of open reading frame and coded for a 64,230‐Da protein. This protein bears 56.2% amino acid homology to the MACS1 (medium‐chain acyl‐CoA synthetase) enzyme, 58.7% homology to the bovine XL‐III XM‐ligase, and 81.5% homology to the bovine XL‐I XM‐ligase. The cDNA could be expressed in COS cells, and the expressed enzyme had greater benzoate activity than phenylacetate activity, which is consistent with the known substrate specificity of HXM‐A. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:1–6, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10056
Increase of lipid peroxidation by cisplatin in WI38 cells but not in SV40‐transformed WI38 cells
Authors: Yen Hsiu‐Chuan , Nien Chung‐Yi , Majima Hideyuki J. , Lee Chia‐Pei , Chen Sz‐Yun , Wei Jeng‐Shu , See Lai‐Chu 
Published: March 2003

AbstractCisplatin (CPT) is an effective anticancer drug that causes cumulative toxicity to normal tissues. It has been suggested that CPT damages normal cells by causing oxidative stress, but it is not known whether it can induce similar oxidative damage to tumor cells. In this study, by using normal human lung fibroblast (W138) cells and SV40‐transformed WI38 (VA13) cells as a model, we compared the effect of CPT on cytotoxicity, apoptosis, lipid peroxidation, and mitochondrial gene expression, which could be regulated by oxidative stress, between normal and tumor cells. CPT induced greater growth inhibition and percentage of apoptotic cells in VA13 cells. However, levels of esterified F2‐isoprostanes and 4‐hydroxy‐2‐nonenal, two specific products of lipid peroxidation, were increased by CPT in WI38 cells, but not in VA13 cells. Furthermore, the transcript level of mitochondrial 12S rRNA was augmented by CPT in both cells, but to a higher degree in WI38 cells. The data suggest a correlation between lipid peroxidation and cytotoxicity or increased mitochondrial transcript levels in WI38 cells but not in VA13 cells. The results also indicate an altered response of oxidative damage and mitochondrial gene regulation to CPT in the transformed phenotype of WI38 cells. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:39–46, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10059
Aryl sulfatase from Naja nigricolis venom: Characterization and possible contribution in the pathology of snake poisoning
Authors: Nok Andrew J. , Abubakar M. S. , Adaudi Ambrose , Balogun E. 
Published: March 2003

AbstractThe venom of Naja nigricolis was found to contain a high level of the enzyme aryl sulfatase. The enzyme was isolated from the venom of N. nigriclois and purified to electrophoretic homogeneity by gel chromatography on Sephadex G‐100, DEAE‐cellulose, and phenyl‐sepharose columns. The enzyme was optimally active at pH 5 and 40°C. Arrhenius plot for the determination of the activation energy (Ea) gave the value 25 kJ/mol with a half‐life (t1/2) of 5 min at 50°C. It was highly activated by Fe2+ and Ca2+ and inhibited by Co2+ and Mn2+. The enzyme catalyzed the hydrolysis of the fluorescent compound methylumbelliferyl‐sulfate (MU‐SO4). Double reciprocal plots of initial velocity data, using MU‐SO4 as substrate, gave a KM value of 110 μM and Vmax of 225 μmol min−1 mg−1. N. nigricolis Aryl sulphatase also hydrolyzed chondroitin‐4‐sulphate. It was inhibited competitively by N‐acetyl glucosamine sulfate (GlcNAc‐SO4), glucose‐6‐sulfate (Glc‐6‐SO4), and glucose 1‐sulfate (Glc‐1‐SO4). Extrapolated inhibition binding constants (Ki) gave the values of 3, 25, and 315 μM for GlcNAc‐SO4, Glc‐6‐SO4, and Glc‐1‐SO4 respectively. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:59–66, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10061
Why capacity trumps costs
Authors: Yu‐Lee Reginald Tomas 
Published: March 2003

AbstractUnderstanding how to reduce costs is not hard. But savings accrue only from acting on improvement ideas, and
only then will there be any financial benefit. Ultimately, companies have to execute effectual management
practices to successfully manage their costs. To effectively utilize capacity, a company's managers must
take extra steps to reduce the capacity or else pay less for it. © 2003 Wiley Periodicals,
Inc.
Performance excellence through activity value management
Authors: Higgins Brian , Young S. Mark 
Published: March 2003

AbstractThis article introduces a new way to think about managing information. This approach, known as
“activity value management,” focuses on improvement methods and uses a coordinated approach to
integrating information. This is necessary because improvement initiatives are often suboptimized simply because
of a lack of coordination. When—as frequently happens—these programs are sponsored by various
organizational silos, they often have to compete for resources, funding, management support, and commitment.
Because no overarching structure exists to link the outcomes of these various improvement efforts, the overall
strategic vision of the enterprise often goes unmet. © 2003 Wiley Periodicals, Inc.
A new look at manufacturing using CAM‐I's capacity management model
Authors: Muras Andrew , Rodriguez Marlene 
Published: March 2003

AbstractA capacity management model helps managers evaluate capacity by providing a visual presentation of problem
areas. This can lead to much‐needed insight to address many management issues in manufacturing and
production. This article outlines an application of the capacity model developed by the Consortium for Advanced
Manufacturing‐International (CAM‐I), along with the synergistic efforts of product
costing and process improvement initiatives. © 2003 Wiley Periodicals, Inc.
Operational resource accounting
Authors: Stenzel Catherine , Stenzel Joe 
Published: March 2003

AbstractOperational resource accounting provides a more integrated, cross‐functional perspective on
organizational resources than conventional analytical methods. Its tenets include the belief that people are the
most important process, product, and purpose; that pricing is a partnership, not a competition; and
that internal projects and initiatives are all about renewal of people and processes. © 2003 Wiley
Periodicals, Inc.
Coordinate down‐regulation of cartilage matrix gene expression in Bcl‐2 deficient chondrocytes is associated with decreased SOX9 expression and decreased mRNA stability
Authors: Kinkel Mary D. , Horton Walter E. 
Published: March 2003

AbstractThe anti‐apoptotic protein Bcl‐2 has been shown to function in roles unrelated to apoptosis in a variety of cell types. We have previously reported that loss of Bcl‐2 expression alters chondrocyte morphology and modulates aggrecan expression via an apoptosis‐independent pathway. Here we show that Bcl‐2 is required for chondrocytes to maintain expression of a variety of cartilage‐specific matrix proteins. Using quantitative, real‐time PCR, we demonstrate that Bcl‐2‐deficient chondrocytes coordinately down‐regulate genes coding for hyaline cartilage matrix proteins including collagen II, collagen IX, aggrecan, and link protein. The decrease in steady‐state level of these mRNA transcripts results, in part, from decreased mRNA stability in Bcl‐2‐deficient chondrocytes. Transcriptional regulation is also likely involved because chondrocytes with decreased Bcl‐2 levels show decreased expression of SOX9, a transcription factor necessary for expressing the major cartilage matrix proteins. In contrast, chondrocytes constitutively expressing Bcl‐2 have a stable phenotype when subjected to loss of serum factor signaling. These cells maintain high levels of SOX9, as well as the SOX9 targets collagen II and aggrecan. These results suggest that Bcl‐2 is involved in a pathway important for maintaining a stable chondrocyte phenotype. J. Cell. Biochem. 88: 941–953, 2003. © 2003 Wiley‐Liss, Inc.
Expression of various genes is controlled by DNA methylation during mammalian development
Authors: Ehrlich Melanie 
Published: March 2003

AbstractDespite thousands of articles about 5‐methylcytosine (m5C) residues in vertebrate DNA, there is still controversy concerning the role of genomic m5C in normal vertebrate development. Inverse correlations between expression and methylation are seen for many gene regulatory regions [Heard et al., 1997; Attwood et al., 2002; Plass and Soloway, 2002] although much vertebrate DNA methylation is in repeated sequences [Ehrlich et al., 1982]. At the heart of this debate is whether vertebrate DNA methylation has mainly a protective role in limiting expression of foreign DNA elements and endogenous transposons [Walsh and Bestor, 1999] or also is important in the regulation of the expression of diverse vertebrate genes involved in differentiation [Attwood et al., 2002]. Enough thorough studies have now been reported to show that many tissue‐ or development‐specific changes in methylation at vertebrate promoters, enhancers, or insulators regulate expression and are not simply inconsequential byproducts of expression differences. One line of evidence comes from mutants with inherited alterations in genes encoding DNA methyltransferases and from rodents or humans with somatically acquired changes in DNA methylation that illustrate the disease‐producing effects of abnormal methylation. Another type of evidence derives from studies of in vivo correlations between tissue‐specific changes in DNA methylation and gene expression coupled with experiments demonstrating cause‐and‐effect associations between DNA hyper‐ or hypomethylation and gene expression. In this review, I summarize some of the strong evidence from both types of studies. Taken together, these studies demonstrate that DNA methylation in mammals modulates expression of many genes during development, causing major changes in or important fine‐tuning of expression. Also, I discuss previously established and newly hypothesized mechanisms for this epigenetic control. J. Cell. Biochem. 88: 899–910, 2003. © 2003 Wiley‐Liss, Inc.
Modeling zinc in biomolecules with the self consistent charge‐density functional tight binding (SCC‐DFTB) method: Applications to structural and energetic analysis
Authors: Elstner Marcus , Cui Qiang , Munih Petra , Kaxiras Efthimios , Frauenheim Thomas , Karplus Martin 
Published: March 2003

AbstractParameters for the zinc ion have been developed in the self‐consistent charge density functional tight‐binding (SCC‐DFTB) framework. The approach was tested against B3LYP calculations for a range of systems, including small molecules that contain the typical coordination environment of zinc in biological systems (cysteine, histidine, glutamic/aspartic acids, and water) and active site models for a number of enzymes such as alcohol dehydrogenase, carbonic anhydrase, and aminopeptidase. The SCC‐DFTB approach reproduces structural and energetic properties rather reliably (e.g., total and relative ligand binding energies and deprotonation energies of ligands and barriers for zinc‐assisted proton transfers), as compared with B3LYP/6‐311+G** or MP2/6‐311+G** calculations. © 2003 Wiley Periodicals, Inc. J Comput Chem 24: 565–581, 2003