bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaccine-induced SARS-CoV-2 antibody response: the comparability of S1-specific binding assays depends on epitope and isotype discrimination
Authors: Schest Silvia , Langer Claus , Stiegler Yuriko , Karnuth Bianca , Arends Jan , Stiegler Hugo , Masetto Thomas , Peter Christoph , Grimmler Matthias 
Published: October 2023

BackgroundQuantification of the SARS-CoV-2-specific immune response by serological immunoassays is critical for the management of the COVID-19 pandemic. In particular, neutralizing antibody titers to the viral spike (S) protein have been proposed as a correlate of protection (CoP). The WHO established the First International Standard (WHO IS) for anti-SARS-CoV-2 immunoglobulin (Ig) (NIBSC 20/136) to harmonize binding assays with the same antigen specificity by assigning the same unitage in binding antibody units (BAU)/ml.MethodIn this study, we analyzed the S1-specific antibody response in a cohort of healthcare workers in Germany (n = 76) during a three-dose vaccination course over 8.5 months. Subjects received either heterologous or homologous prime-boost vaccination with ChAdOx1 nCoV-19 (AstraZeneca) and BNT162b2 (Pfizer-BioNTech) or three doses of BNT162b2. Antibodies were quantified using three anti-S1 binding assays (ELISA, ECLIA, and PETIA) harmonized to the WHO IS. Serum levels of neutralizing antibodies were determined using a surrogate virus neutralization test (sVNT). Binding assays were compared using Spearman’s rank correlation and Passing–Bablok regression.FindingsAll assays showed good correlation and similar antibody kinetics correlating with neutralizing potential. However, the assays show large proportional differences in BAU/ml. ECLIA and PETIA, which detect total antibodies against the receptor- binding domain (RBD) within the S1 subunit, interact similarly with the convalescent plasma-derived WHO IS but differently with vaccine serum, indicating a high sensitivity to the IgG/IgM/IgA ratio.ConclusionAll three binding assays allow monitoring of the antibody response in COVID-19-vaccinated individuals. However, the assay-specific differences hinder the definition of a common protective threshold in BAU/ml. Our results highlight the need for the thoughtful use of conversion factors and consideration of method-specific differences. To improve the management of future pandemics and harmonize total antibody assays, we should strive for reference material with a well-characterized Ig isotype composition.
Comprehensive analysis of necroptosis-related genes in renal ischemia-reperfusion injury
Authors: Li Shuai , Zhang Weixun , Hu Xiaopeng 
Published: October 2023

BackgroundOxidative stress is the primary cause of ischemia-reperfusion injury (IRI) in kidney transplantation, leading to delayed graft function (DGF) and implications on patient health. Necroptosis is believed to play a role in renal IRI. This research presents a comprehensive analysis of necroptosis-related genes and their functional implications in the context of IRI in renal transplantation.MethodsThe necroptosis-related differentially expressed genes (NR-DEGs) were identified using gene expression data from pre- and post-reperfusion renal biopsies, and consensus clustering analysis was performed to distinguish necroptosis-related clusters. A predictive model for DGF was developed based on the NR-DEGs and patients were divided into high- and low-risk groups. We investigated the differences in functional enrichment and immune infiltration between different clusters and risk groups and further validated them in single-cell RNA-sequencing (scRNA-seq) data. Finally, we verified the expression changes of NR-DEGs in an IRI mouse model.ResultsFive NR-DEGs were identified and were involved in various biological processes. The renal samples were further stratified into two necroptosis-related clusters (C1 and C2) showing different occurrences of DGF. The predictive model had a reliable performance in identifying patients at higher risk of DGF with the area under the curve as 0.798. Additionally, immune infiltration analysis indicated more abundant proinflammatory cells in the high-risk group, which was also found in C2 cluster with more DGF patients. Validation of NR-DEG in scRNA-seq data further supported their involvement in immune cells. Lastly, the mouse model validated the up-regulation of NR-DEGs after IR and indicated the correlations with kidney function markers.ConclusionsOur research provides valuable insights into the identification and functional characterization of NR-DEGs in the context of renal transplantation and sheds light on their involvement in immune responses and the progression of IRI and DGF.
One year study on the reproductive biology, ovary characterization and age of the European sardine (Sardina pilchardus) in the middle-west Adriatic Sea
Authors: Chemello Giulia , Cerrone Greta Lucia , Tavolazzi Valentina , Donato Fortunata , Tiralongo Francesco , Gioacchini Giorgia 
Published: October 2023

The ecological and economic relevance of the European sardine (Sardina pilchardus) in the Adriatic area is well established. High exploitation rates and instability of environmental parameters could potentially impair the reproductive physiology of this species, compromising the stock’s stability. To guarantee efficient stock management, there is a need to fill the lack of updated information regarding the structure, sex ratio and reproductive season of the sardine population in the Adriatic Sea. The present study provides new data on the distribution of females’ maturation phases, sex ratio, age frequency and seasonality of the reproductive period in the middle-western Adriatic Sea. Sardine specimens were collected monthly, from April 2021 to March 2022 in the Adriatic waters off the coast of Ancona. Size, weight and sex were determined for a sub-sample of almost 144 specimens during each sampling period. Through otolith analysis and ovary characterization, population age and females’ maturation phase were estimated respectively. The results obtained highlighted a general reduction in size (15.5cm the highest size class) and age (maximum age 2+, greater than 2 years old but less than 3 years) of the population that was characterized by the predominance of males. Also, an interesting scarcity of small female specimens (&lt; 13 cm length) was observed. The reproductive period seemed to occur between October and June as suggested by ovarian characterization, GSI (0.50, 0.30 and 0.62 respectively) and Fulton’s condition factor (0.73, 0.74 and 0.74 respectively) analysis that showed the lowest values in July, August and September corresponding to the rest period.
Neutrophil–lymphocyte ratio and platelet–lymphocyte ratio as potential predictive markers of treatment response in cancer patients treated with immune checkpoint inhibitors: a systematic review and meta-analysis
Authors: Rugambwa Tibera K. , Abdihamid Omar , Zhang Xiangyang , Peng Yinghui , Cai Changjing , Shen Hong , Zeng Shan , Qiu Wei 
Published: October 2023

BackgroundThe role of platelet–lymphocyte ratio (PLR) and neutrophil–lymphocyte ratio (NLR) as independent prognostic markers in different tumors is well established. However, there is a limited review of the potential of NLR and PLR as predictors of treatment outcomes from immune checkpoint inhibitors (ICIs).ObjectiveTo establish a correlation between NLR and PLR and the potential of clinical benefit from ICIs.MethodsThe literature search was performed for studies that reported the association between NLR, PLR, and treatment outcomes among cancer patients treated with ICIs. The outcomes of interest were objective response rate (ORR), disease control rate (DCR), and progressive disease (PD). ORR was the summation of patients who achieved complete response and partial response. DCR included patients who achieved stable disease. PD was the proportion of patients who progressed, relapsed, or discontinued the treatment. Statistical analysis was performed using the STATA 12.0 package. Heterogeneity was determined by the I2 value. Quality assessment was performed using the Newcastle–Ottawa Scale. Egger’s test was used to establish publication bias and sensitivity analysis.ResultsA total of 40 papers that met the inclusion criteria were included in the systematic review. However, only 17 studies were used in the meta-analysis to determine the correlation between NLR, PLR, and treatment response. We found that treatment with ICIs and monitoring of outcomes and adverse events using PLR and NLR parameters have been studied in different tumors. Our analysis showed that low NLR correlated with higher ORR (OR = 0.62 (95% CI 0.47–0.81, p = 0.001) and higher DCR (OR = 0.23, 95% CI 0.14–0.36, p &lt; 0.001). Higher NLR predicted a higher probability of PD (OR = 3.12, 95% CI 1.44, 6.77, p = 0.004). Similarly, low PLR correlated with higher ORR (OR = 0.69, 95% CI 0.5, 0.95, p = 0.025). Generally, patients with low NLR and PLR were more likely to achieve clinical benefit and better response (p-value &lt; 0.001). Meanwhile, patients with high ratios were more likely to progress (p-value &lt; 0.005), although there was significant heterogeneity among studies. There was no significant publication bias observed.ConclusionThe study showed that high NLR and PLR either at baseline or during treatment is associated with poorer treatment outcome. Therefore, these ratios can be utilized in clinical practice with other markers to determine treatment efficacy from immunotherapy.
Efficacy of fosinopril and amlodipine in pediatric primary hypertension: a single-center observational study
Authors: Wang Hui , Shi Lin , Lin Yao , Wang Yuting , Niu Wenquan , Li Yaqi 
Published: October 2023

ObjectiveFosinopril and amlodipine are commonly prescribed as first-line pharmacotherapeutic agents for pediatric hypertension, but there is a lack of comparative studies regarding the efficacy of these two drugs. We aimed to evaluate and compare the efficacy of fosinopril and amlodipine monotherapy in pediatric primary hypertension.MethodsThis was a single-center, bidirectional observational study. A total of 175 children and adolescents with primary hypertension receiving antihypertensive monotherapy from July 2020 to February 2023 were enrolled. According to antihypertensive drugs, they were divided into the fosinopril group (n = 96) and the amlodipine group (n = 79). Subgroup analysis was performed to compare the efficacy of the two groups in terms of blood pressure (BP) control rates and reductions following a 4-week treatment.ResultsAfter 4 weeks of treatment, both groups achieved significant reductions in systolic BP (SBP) and diastolic BP (DBP) by more than 18 mmHg and 6 mmHg, respectively, with BP control rates of 61.5% in the fosinopril group and 59.5% in the amlodipine group, revealing no significant differences in the antihypertensive efficacy between the two groups except for DBP control rate (FDR adjusted P &gt; 0.05). Further subsequent subgroup analyses revealed that the reductions in SBP and DBP in the fosinopril group were significantly greater than those in the amlodipine group in patients of females and hypo-HDL-cholesterolemia (FDR adjusted P &lt; 0.05), and there was a trend of difference, although not significant, in patients with central obesity and insulin resistance (IR) (FDR adjusted 0.05 &lt; P ≤ 0.1). However, there were no significant differences in treatment efficacy in patients without these characteristics. Furthermore, hypertriglyceridemia did not exhibit a significant association with the difference in treatment efficacy between the two medications (FDR adjusted P &gt; 0.05).ConclusionsFosinopril and amlodipine monotherapy were both effective in pediatric primary hypertension during a short-term follow-up. Fosinopril may be particularly effective in reducing BP in hypertensive patients of females, central obesity, IR, and hypo-HDL-cholesterolemia. These findings indicate that optimizing antihypertensive medication selection based on the individualized characteristics of children with hypertension may improve the efficacy of antihypertensive treatment.
THE INFLUENCE OF COMPETENCE-ORIENTED TASKS OF CARTOGRAPHIC DIRECTION ON THE QUALITY OF TEACHING GEOGRAPHY IN HIGHER EDUCATION
Authors: Fedii O. , Halushka L. , Hasanova S. 
Published: October 2023

The article determines that geographic education, especially at the current stage of society’s development, is impossible without theoretical foundations and practical skills in working with cartographic sources, their analysis, and an integrated approach in the fields of application. It has been proven that competence-oriented tasks (COTs) in cartography and topography provide high-quality training for students of the first (bachelor’s) and second (master’s) levels of higher education. An own definition of the COTs of the cartographic direction is proposed. The issue of cartographic teaching aids development has been highlighted, and their conditions have been analyzed within future geography teacher training. The peculiarities of problem-solving method in cartography and topography for students of higher education institutions are revealed. A diagram of the advantages of the COTs of cartographic orientation over traditional tasks has been created according to their application stages. They are the following: life orientation, a motive that leads to a resolution, independence in decision-making and high cognitive complexity. The positive features of training results with the elements of COTs have been specified.
COTs of the cartographic direction have been developed, which can be used during the mastering of the disciplines «Cartography with the Fundamental of Topography», «Methodology of Teaching Geography», and «Methodology of Teaching Geography in a Senior Specialized School» by students of higher education in Poltava V. G. Korolenko National Pedagogical University (PNPU) and Azerbaijan State Pedagogical University (ASPU). Attention is focused on the importance of the topographic map in the wartime period.
INNOVATIVE APPROACHES TO STUDENTS’ KEY COMPETENCIES FORMATION IN THE CONTEXT OF LABOR TRAINING
Authors: Hrytsenko L. , Liashenko S. 
Published: October 2023

The article is devoted to research of innovative approaches to students’ key competencies formation in the context of labor training. The importance of using innovative methods and technologies in the learning process, in particular, those based on the active involvement of students, contributing to the development of their skills, knowledge, and abilities, which are key to a successful further professional career, is determined. The types of competencies are singled out: general cultural, civic, health-preserving, social, information-communication, and project-technological. Attention is also paid to interactive learning, which stimulates students’ active participation in the learning process and promotes the development of their creative thinking and problem-solving. The importance of using information and communication technologies (ICT) in the educational process is emphasized, as they allow lecturers to effectively involve students in activities, stimulate independent learning, and promote the development of digital literacy.
In general, the article considers innovative approaches to students’ key competencies formation in the context of labor training, emphasizing the importance of using innovative methods, a competency approach, interactive learning, and information and communication technologies. It has been determined that innovative approaches usage in labor education is the main element for the efficient students’ key competencies formation, such as knowledge, skills, and developed creative abilities. It is noted that the systematic implementation of innovative approaches in labor training guarantees a smooth and consistent development of student’s skills and competencies, which makes the learning process more understandable and attractive for them. It is substantiated that thanks to innovative approaches, education quality improves significantly. It helps students to prepare for life in the modern information society, where the requirements for competencies are constantly changing.
Strategi Penataan Lingkungan Berbasis Masyarakat Di Kawasan Pantai Kastela Ternate, Maluku Utara
Authors: Handiwiyanto Raras , Haris Muh , Rizkiyanto M. Taher  
Published: October 2023

Abstrak
Kota Ternate merupakan salah salah satu Kota yang memiliki destinasi wisata bahari yang potensial, salah satunya adalah Pantai Kastela yang terletak di keluarahan Kastela. Kondisi lingkungan di sekitar pantai Kastela yang kurang tertata menjadi salah satu hal yang perlu dibenahi untuk mengembangkan pariwisata pantai Kastela. Oleh karena itu CSR PT Pertamina Patra Niaga Fuel Terminal Ternate menyelenggarakan program penataan lingkungan Kawasan Pantai Kastela. Program ini bertujuan untuk menata kawasan wisata Ring 1 Pertamina dengan melibatkan peran serta masyarakat melalui pembentukan pokdarwis. Program penataan lingkungan di Kawasan Pantai Kastela diselenggarakan dengan menggunakan strategi kegiatan focus group discussion (FGD) dan perencanaan kerja, sosialisasi penataan lingkungan, pengukuhan kelompok sadar wisata, pelatihan manajemen wisata, pengadaan infastruktur, dan Kerjasama dengan berbagai instansi.
Kata Kunci: Penataan; Lingkungan; Pantai Kastela; Berbasis Masyarakat
Contexte scolaire et mixité Pistes de réflexion en psychologie sociale
Authors: Morin-Messabel Christine 
Published: October 2023

Avant d’interroger la variable « mixité », il est nécessaire de définir les mécanismes liés à la catégorisation de sexe, à savoir l’asymétrie cognitive et sociale et ses conséquences sur les différenciations entre groupes de sexe régis par un rapport de pouvoir.
Des éclairages théoriques de psychologie sociale sur la variable sexe et le système éducatif et, d’autre part, des éléments de réflexion sur les contextes pédagogiques d’apprentissage.
Les mutations dans le métier d'éducateur de jeunes enfants
Authors: Verba Daniel 
Published: October 2023

Le métier d'éducateur de jeunes enfants restera durablement marqué par d'importantes mutations, avec la décentralisation d'une part, les évolutions sociologiques de la famille d'autre part, et une série d'infléchissements juridiques, notamment par la refonte du diplôme d'État en 1993-2005 et surtout la publication, après vingt ans de tergiversations et de luttes d'influence (1980-2000), du décret « petite enfance ».
Il convient d'analyser les effets de ces mutations sur les pratiques et l'identité professionnelles des éducateurs et les défis à relever pour les années futures.
Les loisirs des jeunes dans la cité
Authors: Charbonnel Pierre 
Published: October 2023

Dans le cadre de l'ouvrage « Les Jeunes et leurs loisirs », l'Observatoire de l'enfance en France a mené en 2003 une enquête originale auprès de 1 276 collégiens et lycéens.
Cette enquête démontre que les loisirs des jeunes sont une composante non négligeable de leur rapport à la cité et que, à côté des infrastructures indispensables (installations sportives, culturelles, d'accueil), il conviendrait d'envisager des actions de type volontariste.
De la ségrégation urbaine à la ségrégation scolaire Propos d’un économiste
Authors: Maurice Joël 
Published: October 2023

La question de la ségrégation urbaine, peu abordée par les économistes, l’a cependant été dans le rapport Fitoussi-Laurent-Maurice.
L’un des auteurs présente les sept éléments de cette analyse économique, qui ont permis de déboucher sur un ensemble de recommandations, dont certaines concernent l’école.
Creil (Oise) Collège Gabriel-Havez. Dispositif d'accueil et d'intégration
Authors: Laborie Henri 
Published: October 2023

Les élèves nouvellement arrivés en France sont, dans leur quasi totale majorité, d’une origine linguistique autre que le français.
Or, s’il suffit de quelques mois d’immersion pour acquérir un début de compétence orale suffisante pour communiquer en français dans le cadre de la vie quotidienne, cette compétence – qui ne repose que sur le système de l’oral – s’avère totalement insuffisante lorsqu’il s’agit de l’acquisition de savoirs scolaires qui, eux, reposent essentiellement sur le système de l’écrit.
C’est pourquoi, si un dispositif spécifique d’enseignement du français langue étrangère n’est pas proposé, la langue des disciplines, au travers de laquelle doivent se faire les acquisitions scolaires thématiques constitue plus un barrage qu’une voie de transfert.
Le collège Gabriel-Havez, à Creil, collège classé « ambition réussite », a donc été amené, comme d’autres établissements, à créer des dispositifs spécifiques pour accueillir ces élèves.
Lyon (Rhône) La Cité scolaire internationale. FLS et utilisations diversifiées de l'environnement homoglotte
Authors: Duc-Ballatore Monique , Bouchard Christine 
Published: October 2023

En inscrivant leurs enfants à la Cité scolaire internationale (CSI) de Lyon, les parents marquent leur volonté de poursuivre en France un projet plurilingue d’autant plus nécessaire que la durée de leur séjour est variable et soumise aux déplacements pour raisons professionnelles de « travailleurs migrants temporaires ».
La plupart du temps, il ne s’agit ni du premier ni du dernier déménagement, même si certaines familles souhaitent se fixer définitivement en France…
Dans ce contexte, les élèves ont des parcours scolaires, des acquis linguistiques, des projets à court, moyen et long termes extrêmement variés.
Le dispositif Adultes relais
Authors: Ruffion Alain 
Published: October 2023

Le dispositif Adultes relais, au début des années 2000, a offert la possibilité à plus de 4 000 médiateurs d’exercer dans le champ polymorphe des médiations socioculturelles. Ces neuf années d’existence donnent suffisamment de recul, à l’heure ou l’Agence nationale pour la cohésion sociale et l’égalité des chances (ACSÉ) évalue ce dispositif pour cerner l’utilité sociale de leurs actions. Le temps semble propice également à la mesure de leurs influences sur les politiques publiques même si ces influences restent confrontées aux limites artificielles que l’on impose trop souvent à la médiation enfermée dès lors dans des conceptions sécuritaires ou pacificatrices
Un français pour les jeunes Algériens
Authors: Hedid Souheila 
Published: October 2023

L'image utilisée par H. Boyer pour expliquer la complexité de ce que l'on appelle le parler des jeunes est assez fidèle à sa réalité. Ce parler est tout d'abord un volcan puissant et incontrôlable, bouillonnant car il est en perpétuel changement linguistique. Le parler des jeunes, fruit de l'expansion des sociétés modernes et de leur éclatement identitaire, tend aujourd'hui à se propager et à s'inscrire comme «une forme contemporaine de parler populaire».
Cette forme, souvent caricaturée par les tenants de la norme académique car menaçant le bon usage de la langue standard, constitue pour certains le signe d''un handicap linguistique et pour d'autres une source inépuisable de créativités langagières.
Les manuels scolaires au temps de la colonisation (1830-1962)
Authors: Boutan Pierre 
Published: October 2023

Les manuels scolaires sont souvent utilisés comme moyen de démonstration, à charge en général, dans des procès à l’égard de telle ou telle opinion, en particulier à l’égard de la colonisation : souvent sans précaution. Rappelons des évidences. D’abord, que les manuels ne sont pas l’enseignement : témoins certes concrets et privilégiés, ils constituent un moyen d’information commode mais qui mérite des recoupements pour en apprécier les effets réels. L’acculturation, ensuite, passe par bien d’autres chemins que l’école. Pour autant, la pyramide des âges permet d’estimer à 20 % de la population française (ayant eu 8 ans au moins en 1958) la proportion qui est allée à l’école avant 1962 et a donc utilisé les manuels scolaires d’avant la fin de la colonisation.