bluuebunny/crossref_metadata_2025_split huggingface dataset:
PD-1 signaling uncovers a pathogenic subset of T cells in inflammatory arthritis
Authors: Straube Johanna , Bukhari Shoiab , Lerrer Shalom , Winchester Robert J. , Gartshteyn Yevgeniya , Henick Brian S. , Dragovich Matthew A. , Mor Adam 
Published: January 2024

Abstract
Background
PD-1 is an immune checkpoint on T cells, and interventions to block this receptor result in T cell activation and enhanced immune response to tumors and pathogens. Reciprocally, despite a decade of research, approaches to treat autoimmunity with PD-1 agonists have only had limited successful. To resolve this, new methods must be developed to augment PD-1 function beyond engaging the receptor.

Methods
We conducted a flow cytometry analysis of T cells isolated from the peripheral blood and synovial fluid of patients with rheumatoid arthritis. In addition, we performed a genome-wide CRISPR/Cas9 screen to identify genes associated with PD-1 signaling. We further analyzed genes involved in PD-1 signaling using publicly available bulk and single-cell RNA sequencing datasets.

Results
Our screen confirmed known regulators in proximal PD-1 signaling and, importantly, identified an additional 1112 unique genes related to PD-1 ability to inhibit T cell functions. These genes were strongly associated with the response of cancer patients to PD-1 blockades and with high tumor immune dysfunction and exclusion scores, confirming their role downstream of PD-1. Functional annotation revealed that the most significant genes uncovered were those associated with known immune regulation processes. Remarkably, these genes were considerably downregulated in T cells isolated from patients with inflammatory arthritis, supporting their overall inhibitory functions. A study of rheumatoid arthritis single-cell RNA sequencing data demonstrated that five genes, KLRG1, CRTAM, SLAMF7, PTPN2, and KLRD1, were downregulated in activated and effector T cells isolated from synovial fluids. Backgating these genes to canonical cytotoxic T cell signatures revealed PD-1+ HLA-DRHIGH KLRG1LOW T cells as a novel inflammatory subset of T cells.

Conclusions
We concluded that PD-1+ HLA-DRHIGH KLRG1LOW T cells are a potential target for future PD-1 agonists to treat inflammatory diseases. Our study uncovers new genes associated with PD-1 downstream functions and, therefore, provides a comprehensive resource for additional studies that are much needed to characterize the role of PD-1 in the synovial subset of T cells.
Exploring a holistic training program on tactical behavior and psychological components of elite soccer players throughout competition season: a pilot study
Authors: Tassi Juan M. , Nobari Hadi , García Jesús Diaz , Rubio Ana , Gajardo Miguel Ángel López , Manzano David , García-Calvo Tomás 
Published: January 2024

AbstractThis study aimed to assess the effects of a training program, considering an ecological/holistic perspective, on both tactical behavior (i.e., principles of play; PP) and psychological aspects (i.e., emotional, and cognitive components) in elite soccer teams consisting of players from the U23 and U21 age groups. The participants were 46 players from the under U-23 and U-21 teams from the same club and the first division of Argentina. A quasi-experimental design was examined after five weeks of intervention through integrated training tasks, where psychological factors were used for the development of the tactical principles of the game. Two evaluations of the improvement of game principles were performed before and after the intervention. The results showed significant changes and differences in both teams concerning the PP during the post-intervention period. Specifically, the results show significant group-by-time interactions with an increase in the percentage of tactical actions and behaviors performed during the competitions in two game principles analyzed in both teams. There were significant in the group-by-time interactions for PP 3 (i.e., the first option to pass forward; p ≤ 0.001, F = 58.96, ηp2 = 0.88) and also, significant changes were in PP 4 (i.e., immediate pressure when losing the ball) through the main effect of time (p ≤ 0.001, F = 105.41, ηp2 = 0.93) and group by time interactions (p = 0.002, F = 20.08, ηp2 = 0.72). In both groups, there were significant changes in post hoc analysis (PP3: U21: p = 0.039 vs. U23: p ≤ 0.001) and (PP4: U21: p = 0.006 vs. U23: p = 0.001). It seems the strategies and constraints used, tactical components, and integrated into the psychological aspects during specific soccer training tasks can help improve the tactical behaviors of both teams in a competition associated with the PP of a soccer team.
Kitesurfing and snowkiting injuries in Norway: a retrospective study
Authors: Torland Venke , Thomassen Øyvind , Østerås Øyvind 
Published: January 2024

Abstract
Background
Kiteboarding (kitesurfing on water and snowkiting) is a fairly new sport and is defined as a high-risk sport. The injury rate has been reported to be between 6 and 9 per 1000 h. The aim of the study was to identify and describe kiteboarding-related injuries in Norway over a five-year period.

Methods
We used “snowball sampling” to identify kiteboarding accidents in a retrospective study. In addition, we conducted structural searches in the National Air Ambulance Service and Search and Rescue Helicopter patient record databases. All included informants were interviewed. Descriptive methods were used to characterise the sample.

Results
Twenty-nine kiteboarders were included, with a total of 33 injuries. One half of the injuries to head, face and neck were cerebral concussions (n = 12). The most common type of injury was bone fractures (n = 28), followed by soft tissue injuries (n = 24). Most injuries were of moderate severity (51%) and falling from less than 5 m was the most common mechanism of injury. Operator error and lack of experience were the most frequently reported causes of accidents (82%).

Conclusions
Serious injuries occured during kiteboarding. The majority of kiteboarders reported operator error or lack of experience as the cause of their accident. Prior to kiteboarding, a course highlighting the importance in using helmet for snowkiting and both helmet and life vest in kitesurfing, should be mandatory.
Plasma metabolic profiles predict future dementia and dementia subtypes: a prospective analysis of 274,160 participants
Authors: Qiang Yi-Xuan , You Jia , He Xiao-Yu , Guo Yu , Deng Yue-Ting , Gao Pei-Yang , Wu Xin-Rui , Feng Jian-Feng , Cheng Wei , Yu Jin-Tai 
Published: January 2024

AbstractBackgroundBlood-based biomarkers for dementia are gaining attention due to their non-invasive nature and feasibility in regular healthcare settings. Here, we explored the associations between 249 metabolites with all-cause dementia (ACD), Alzheimer’s disease (AD), and vascular dementia (VaD) and assessed their predictive potential.MethodsThis study included 274,160 participants from the UK Biobank. Cox proportional hazard models were employed to investigate longitudinal associations between metabolites and dementia. The importance of these metabolites was quantified using machine learning algorithms, and a metabolic risk score (MetRS) was subsequently developed for each dementia type. We further investigated how MetRS stratified the risk of dementia onset and assessed its predictive performance, both alone and in combination with demographic and cognitive predictors.ResultsDuring a median follow-up of 14.01 years, 5274 participants developed dementia. Of the 249 metabolites examined, 143 were significantly associated with incident ACD, 130 with AD, and 140 with VaD. Among metabolites significantly associated with dementia, lipoprotein lipid concentrations, linoleic acid, sphingomyelin, glucose, and branched-chain amino acids ranked top in importance. Individuals within the top tertile of MetRS faced a significantly greater risk of developing dementia than those in the lowest tertile. When MetRS was combined with demographic and cognitive predictors, the model yielded the area under the receiver operating characteristic curve (AUC) values of 0.857 for ACD, 0.861 for AD, and 0.873 for VaD.ConclusionsWe conducted the largest metabolome investigation of dementia to date, for the first time revealed the metabolite importance ranking, and highlighted the contribution of plasma metabolites for dementia prediction.
Rare, late onset of immune checkpoint inhibitor-induced type 1 diabetes mellitus in a patient with small-cell lung cancer treated with serplulimab: a case report and review of the literature
Authors: Ning Peng , Liu Shilan , Cao Hongyi 
Published: January 2024

Abstract
Background
As a newly approved immune checkpoint inhibitor in China, serplulimab has been widely used in the immunotherapy of tumors. However, the immune-related adverse events of immune checkpoint inhibitors should not be ignored. Although immune checkpoint inhibitor-induced type 1 diabetes mellitus is a rare complication, it may cause diabetic ketoacidosis and endanger the lives of patients.

Case presentation
This case report describes a 55-year-old male of Han nationality from China diagnosed with small-cell lung cancer with multiple metastases who experienced an adverse event of type 1 diabetes mellitus 68 weeks after receiving serplulimab therapy. The patient presented with typical symptoms of diabetic ketoacidosis, including severe thirst, nausea, vomiting, deep respirations, and stupor. Despite the absence of diabetes-related autoantibodies, the patient had extremely low levels of insulin and C-peptide release. Other potential causes of diabetes were ruled out, confirming the condition as serplulimab-induced immune checkpoint inhibitor-induced type 1 diabetes mellitus. After aggressive treatment to correct diabetic ketoacidosis, the patient’s blood glucose levels stabilized and symptoms of diabetes improved significantly, although long-term insulin maintenance therapy was necessary.

Conclusion
This case highlights a rare, late-onset adverse event of immune checkpoint inhibitor-induced type 1 diabetes mellitus that may be overlooked during treatment with serplulimab. The monitoring of blood glucose levels and early signs and symptoms of diabetes cannot be relaxed at the late stage of treatment, even if patients do not have elevated blood glucose levels before and during the middle stage of treatment.
In silico analysis of the Val66Met mutation in BDNF protein: implications for psychological stress
Authors: Shan Muhammad Adnan , Khan Muhammad Umer , Ishtiaq Warda , Rehman Raima , Khan Samiullah , Javed Muhammad Arshad , Ali Qurban 
Published: January 2024

AbstractThe brain-derived neurotrophic factor (BDNF) involves stress regulation and psychiatric disorders. The Val66Met polymorphism in theBDNFgene has been linked to altered protein function and susceptibility to stress-related conditions. This in silico analysis aimed to predict and analyze the consequences of the Val66Met mutation in theBDNFgene of stressed individuals. Computational techniques, including ab initio, comparative, and I-TASSER modeling, were used to evaluate the functional and stability effects of the Val66Met mutation in BDNF. The accuracy and reliability of the models were validated. Sequence alignment and secondary structure analysis compared amino acid residues and structural components. The phylogenetic analysis assessed the conservation of the mutation site. Functional and stability prediction analyses provided mixed results, suggesting potential effects on protein function and stability. Structural models revealed the importance ofBDNFin key biological processes. Sequence alignment analysis showed the conservation of amino acid residues across species. Secondary structure analysis indicated minor differences between the wild-type and mutant forms. Phylogenetic analysis supported the evolutionary conservation of the mutation site. This computational study suggests that the Val66Met mutation inBDNFmay have implications for protein stability, structural conformation, and function. Further experimental validation is needed to confirm these findings and elucidate the precise effects of this mutation on stress-related disorders.
Hand hygiene compliance in a Brazilian COVID-19 unit: the impact of moments and contact precautions
Authors: Valim Marília Duarte , Rossetto Jéssica Regina , Bortolini Juliano , Herwaldt Loreen 
Published: January 2024

Abstract
Background
Healthcare-associated infections are among the most common complications during hospitalization. These infections increase morbidity and mortality and they increase length of hospital stay and the cost of healthcare. The aims of our study were to monitor hand hygiene (HH) compliance, HH technique quality and factors related to HH practice among health professionals in a COVID-19 Intensive Care Unit (ICU).

Methods
An observational, prospective study. Between September and December 2021, we observed 69 healthcare professionals in an eight-bed ICU for patients with COVID-19 in midwestern Brazil. We used the WHO observation form to collect data. The dependent variable was HH compliance and independent variables were professional category, sex, HH quality (3-step technique for at least 15 s), number of HH opportunities observed, observation shift and inappropriate glove use.

Results
We observed 1185 HH opportunities. The overall compliance rate was 26.4%, but only 6.5% were performed with the correct 3-step technique for the minimum time. HH compliance was considerably lower for moments “before” tasks (6.7%; 95% CI 4.8%, 9.2%) compared with moments “after” tasks (43.8%; 95% CI 39.9%, 47.8%). The logistic model found that inappropriate glove use, night shift and physicians (p < 0.001) were associated with low HH compliance. The infrastructure analysis found that the unit had an insufficient number of alcohol-based handrub (ABHR) dispensers at the point of care and that the mechanism for activating them was poorly designed.

Conclusions
HH compliance was very low. Inappropriate glove use was associated with low compliance and the unit’s infrastructure did not support good HH practice. The fact that healthcare professionals were more likely to do HH after tasks, suggests that they use HH to protect themselves rather than the patients. Adequate infrastructure and ongoing health education with a focus on HH while caring for patients in contact precautions are essential for improving HH compliance and patient safety.
Supporting emergency service workers to cope with critical incidents that can lead to psychological burden at work - developing skills in the Post Critical Incident Seminar: a qualitative interview study
Authors: Korpela Sanna , Nordquist Hilla 
Published: January 2024

Abstract
Background
Emergency service workers face critical incidents causing psychological burden. This qualitative study investigates how attending a Post Critical Incident Seminar could affect the skills of emergency service workers to overcome incidents that can cause psychological burden at work and their consequences with the following research questions: (1) How did attending the Post Critical Incident Seminar impact the skills to overcome work incidents that can lead to psychological burden? and (2) How have these skills been manifested since attending the Post Critical Incident Seminar?

Methods
The data consists of individual interviews with fifteen emergency services workers who attended a Post Critical Incident Seminar in April 2021 in Finland. The interviews were conducted six months after the Post Critical Incident Seminar and analysed using inductive content analysis.

Results
The results of the first research question formed two main categories: improved performance abilities and improved self-regulation abilities. From the results of the second research question two main categories were created: new kind(s) of well-being and readiness to help. Several upper categories and sub-categories were discovered.

Conclusions
Based on the results, attending a Post Critical Incident Seminar may be effective in developing skills to overcome critical incidents that can lead to psychological burden. Further follow-up studies should investigate how acquired skills from the Post Critical Incident Seminar practically affect emergency service workers and their well-being in the longer term.
Effect of lignin in cellulose nanofibers on biodegradation and seed germination
Authors: Stocker Craig W. , Wong Vanessa N. L. , Patti Antonio F. , Garnier Gil 
Published: January 2024

AbstractPure cellulose nanofibers (CNFs) rapidly degrade in soil, limiting their prospective applications in agriculture. We incorporated lignin into CNFs as an antimicrobial and crosslinking agent to control the biodegradation rate. CNFs with different lignin concentrations were prepared by mechanochemical treatment in the presence of choline chloride-urea deep eutectic solvent. These were characterized using conductometric titration, scanning electron microscopy, and FT-IR. The fibers were applied to soil to determine the effect of lignin on soil respiration and nanocellulose degradation, and were used as a substrate for radish and cress seed germination. Modifying the lignin content of the fibers successfully modulated the biodegradation rate in soil. Fibers containing 35% lignin degraded 5.7% in 14 days, while fibers with 20% lignin degraded 20.8% in 14 days. Nanofiber suspensions showed low chemical inhibition for the germination of radish and cress seeds but higher lignin contents reduced the imbibition rate as a seed coating. This study presents the first use of lignin to control the biodegradation rate of cellulose nanofibers in a one-pot, scalable and sustainable system, allowing the advancement of lignocellulose nanofibers for applications such as seed coatings, mulches, and controlled release fertilizers.
Graphical Abstract
Detoxification and enhancement of in vitro rumen digestibility of exhausted olive pomace wastes through alkaline hydrogen peroxide treatment
Authors: Masmoudi Rahma , Ben Yahmed Nesrine , Moujahed Nizar , Darej Cyrine , Smaali Issam 
Published: January 2024

Abstract
Background
Due to the sharp rise in animal feed costs, funding alternatives to substitute high-cost raw materials used in animal feed is a persistent need. This study investigated the effect of alkaline hydrogen peroxide pretreatment as straightforward non-toxic technology to enhance the in vitro rumen digestibility of exhausted olive pomace (EOP), an abundant agricultural waste, to be suitable as animal feedstock. It examined the efficiency to eliminate the toxic phenolic content and minimize lipid oxidation of EOP.

Results
The pretreatment was first optimized using a central composite experimental design. Under the optimized conditions (1.6% H2O2, 5% NaOH), the measured phenolic content was 1.51 ± 0.03 mg/100 g dry weight (DW) for treated olive pomace (TOP) versus 4.91 ± 0.06 mg/100 g for the untreated one. The pretreatment showed that approximately 25% of the lignin was removed. Crude proteins, neutral detergent fibers, and acid detergent fibers yields of TOP were, respectively, 3.320 ± 0.05, 75.24 ± 0.23, and 54.05 ± 0.35 g/100 g of DW, significantly more important than those of untreated EOP. The enzymatic hydrolysis with a cellulase-based cocktail (Celluclast15 FPU/gDW), recorded a 48% of reducing sugar yield for TOP against 33% for EOP. When the in vitro organic matter digestibility (IVOMD) was assayed, the potential gas production of TOP (41.371 ml/g DM) was significantly higher than EOP (25.771 ml/g DM). The metabolizable energy of TOP (9.28 kcal/kg DM) was higher than that of EOP (7.78 kcal/kg DM).

Conclusions
The present study revealed that alkaline hydrogen peroxide (AHP) could be an efficient treatment for the detoxification and enhancement of in vitro rumen digestibility of olive pomace. This straightforward approach demonstrated that treated olive pomace waste may be well valorized as suitable animal feedstock.

Graphical Abstract
Simulation study of atmosphere–ionosphere variations driven by the eruption of Hunga Tonga-Hunga Ha’apai on 15 January 2022
Authors: Shinagawa Hiroyuki , Miyoshi Yasunobu 
Published: January 2024

AbstractThe volcano of Hunga Tonga-Hunga Ha’apai in Tonga erupted on 15 January 2022, generating severe disturbances in the atmosphere and the ionosphere. This event provided us with large amount of data of the atmosphere and the ionosphere, and various kinds of observational studies have been made. Recently several simulation studies have also been made to reproduce and understand the atmosphere–ionosphere variations driven by the volcanic eruption. Although the simulation studies have reproduced the global variations of the atmosphere and the ionosphere successfully, phenomena related with acoustic waves have not been fully investigated. We employed an axisymmetric three-dimensional nonhydrostatic atmospheric model and the whole atmosphere–ionosphere coupled model GAIA. We found that the simulation can produce various kinds of atmospheric waves generated by the eruption, such as acoustic waves, gravity waves, Lamb waves, Pekeris waves, and TIDs concentrically propagating from the eruption site, and atmospheric oscillations with a period of a few minutes. In addition, the results indicate that the eruption generates supersonic shock waves in the volcanic region, leading to the extremely large vertical oscillations in the thermosphere and ionosphere above the volcanic eruption region.
Graphical Abstract
Bathymetric effect on geoid modeling over the Great Lakes area
Authors: Li Xiaopeng , Lin Miao , Krcmaric Jordan , Carignan Kelly 
Published: January 2024

AbstractBathymetry data over lake areas are not included in the current and previous NGS (National Geodetic Survey) geoid models. Lake surfaces are simply treated as land surfaces during the modeling regardless of the apparent density difference between water and rock, resulting in artificial masses that distort the model from the actual gravity field and the corresponding geoid surface. In this study, compiled high-resolution bathymetry data provided by National Centers for Environmental Information are used to identify the real volume of water bodies. Under the mass conservation principle, two strategies are deployed to properly account the water body bounded by the mean lake surface and the bathymetry indicated lake floor into the current NGS geoid modeling scheme, where the residual terrain modeling method is used to account for topographic effects. The first strategy condenses water bodies into equivalent rock masses, with the cost of changing the geometrical shape of the water body. The second one keeps the shape of the water body unchanged but replaces the water and rock densities inside each topographical column bounded by the geoid surface and the mean lake surface by an averaged density. Both strategies show up to 1-cm geoid changes when compared with the previous geoid model that does not consider bathymetric information. All three geoid models are evaluated by local GNSS/Leveling benchmarks and multi-year-multi-mission altimetry indicated mean lake surface heights. The results show that both strategies can improve the geoid model precision. And the second strategy yields more realistic results.Graphical Abstract