bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure of cattle to tick-borne encephalitis virus in the historical endemic zone in north-eastern France
Authors: Mathews-Martin Laure , Gonzalez Gaëlle , Dheilly Nolwenn M. , Amaral-Moraes Rayane , Dumarest Marine , Helle Teheipuaura , Migne Camille , Caillot Christophe , Lacour Sandrine A. , Pérelle Sylvie , Beck Cécile , Metras Raphaëlle , Bournez Laure 
Published: October 2023

Abstract
Background: Tick-borne encephalitis (TBE) is a severe human neuroinfection caused by TBE virus (TBEV). TBEV is transmitted by tick bites and by the consumption of unpasteurized dairy products from infected asymptomatic ruminants. In France, several food-borne transmission events have been reported since 2020, raising the question of the level of exposure of domestic ungulates to TBEV. In this study, our objectives were (i) to estimate TBEV seroprevalence and quantify antibodies titres in cattle in the historical endemic area of TBEV in France using the micro virus neutralisation test (MNT) and (ii) to compare the performance of two veterinary cELISA kits with MNT for detecting anti-TBEV antibodies in cattle in various epidemiological contexts. A total of 344 cattle sera from four grid cells of 100 km² in Alsace-Lorraine (endemic region) and 84 from western France, assumed to be TBEV-free, were investigated.
Results: In Alsace-Lorraine, cattle were highly exposed to the virus with an overall estimated seroprevalence of 57.6% (95% CI: 52.1-62.8%, n=344), varying locally from 29.9% (95% CI: 21.0-40.0%) to 92.1% (95% CI: 84.5-96.8%). Seroprevalence did not increase with age, with one- to three-year-old cattle being as highly exposed as older ones, suggesting a short-life duration of antibodies. The proportion of sera with MNT titres lower than 1:40 per grid cell decreased with increased seroprevalence. Both cELISA kits showed high specificity (>90%) and low sensitivity (less than 78.1%) compared with MNT. Sensitivity was lower for sera with neutralising antibodies titres below 1:40, suggesting that sensitivity of these tests varied with local virus circulation intensity.
Conclusions: Our results highlight that screening strategy and serological tests should be carefully chosen according to the purpose of the serological study and with regard to the limitations of each method.
Efficacy and safety of re-administration of epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) after EGFR-TKI-induced interstitial lung disease (CS-Lung-005)
Authors: Kanaji Nobuhiro , Ichihara Eiki , Tanaka Takaaki , Ninomiya Takashi , Kozuki Toshiyuki , Ishikawa Nobuhisa , Nishii Kazuya , Shoda Hiroyasu , Yamaguchi Kakuhiro , Kawakado Keita , Toyoda Yuko , Inoue Masaaki , Miyatake Nobuyuki , Watanabe Naoki , Inoue Takuya , Mizoguchi Hitoshi , Komori Yuta , Kojima Kazuki , Kadowaki Norimitsu 
Published: October 2023

Abstract
Purpose
This study investigated the safety and efficacy of epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) re-administration after recovery from EGFR-TKI-induced interstitial lung disease (ILD).
Methods
This multicenter retrospective study collected data from consecutive advanced NSCLC patients who underwent EGFR-TKI re-administration after recovery from EGFR-TKI-induced ILD.
Results
Fifty-eight patients were registered. The grades of initial TKI-induced ILD were grade 1 to 4. TKIs used for re-administration were erlotinib for 15 patients, osimertinib for 15, gefitinib for 14, afatinib for 13 patients, and dacomitinib for 1 patient. ILD recurred in 13 patients (22.4%), comprising 3 patients with grade 1, 6 patients with grade 2, and 4 patients with grade 3. No significant associations were found between ILD recurrence and age, smoking history, performance status, time from initial ILD to TKI re-administration, or concomitant corticosteroid use. However, the incidence of ILD recurrence was high in cases of repeated use of gefitinib or erlotinib or first-time use of osimertinib at TKI re-administration. The ILD recurrence rate was lowest in patients treated with first-time use of gefitinib (8%) or erlotinib (8%), followed by patients treated with repeated use of osimertinib (9%). The response rate, median progression-free survival by TKI re-administration, and median overall survival were 55%, 9.6 months, and 84.8 months, respectively.
Conclusion
This study showed that EGFR-TKI re-administration is a feasible and effective treatment for patients who recovered from EGFR-TKI-induced ILD. Our results indicate that re-administration of EGFR-TKI is an important option for long-term prognosis after recovery from EGFR-TKI-induced ILD.
Expression of the CIC-DUX4 fusion oncoprotein mimics human CIC-rearranged sarcoma in genetically engineered mouse models
Authors: Kirsch David , Hendrickson Peter , Oristian Kristianne , Browne MaKenna , Lou Lixia , Ma Yan , Cardona Dianna , Linardic Corinne 
Published: October 2023

Abstract
CIC-DUX4 sarcoma (CDS) is a rare but highly aggressive undifferentiated small round cell sarcoma driven by a fusion between the tumor suppressor Capicua (CIC) and DUX4. Currently, there are no effective treatments and efforts to identify and translate better therapies are limited by the scarcity of patient tumor samples and cell lines. To address this limitation, we generated three genetically engineered mouse models of CDS (Ch7CDS, Ai9CDS, and TOPCDS). Remarkably, chimeric mice from all three conditional models developed spontaneous tumors and widespread metastasis in the absence of Cre-recombinase. The penetrance of spontaneous (Cre-independent) tumor formation was complete irrespective of bi-allelic CIC function and the distance between loxP sites. Characterization of primary and metastatic mouse tumors showed that they consistently expressed the CIC-DUX4 fusion protein as well as other downstream markers of the disease credentialing these models as CDS. In addition, tumor-derived cell lines were generated and ChIP-seq was preformed to map fusion-gene specific binding using an N-terminal HA epitope tag. These datasets, along with paired H3K27ac ChIP-seq maps, validate CIC-DUX4 as a neomorphic transcriptional activator. Moreover, they are consistent with a model where ETS family transcription factors are cooperative and redundant drivers of the core regulatory circuitry in CDS.
The effect of intraoperative intermittent pneumatic compression on deep venous thrombosis prophylaxis in patients undergoing craniotomy
Authors: Qi Xiang , Wang mengrui , Feng kang , Ma Yu , Zhang dan , Guo yidi , Fan Yujie , Jiao yubing , Zhang Xiao yu , wang baoguo , Shi zhonghua , Sun yongxing 
Published: October 2023

Abstract
Background: Postoperative deep venous thrombosis (DVT) is commonly observed in patients undergoing craniotomy and is associated with a high incidence of pulmonary embolism and poor clinical outcomes. Herein, we investigated the prophylactic effect of DVT of intraoperative intermittent pneumatic compression (IPC) in patients undergoing craniotomy.
Methods: A total of 516 patients who underwent elective craniotomy between December 2021 and December 2022 were enrolled in this study. Patients were randomly assigned to the intervention group (received intraoperative IPC) or control group (without IPC). Lower extremity ultrasound was performed on both legs before and after surgery (1h, 24h, and 7 days post-intervention). DVT was defined as the visualization of a thrombus within the vein lumen of the leg. Coagulation and platelet function were measured at the start and end of the craniotomy.
Results: A total of 504 patients (251 in the intervention group and 253 in the control group) completed the study. Among these patients, 20.4% (103/504) developed postoperative DVT within the first week after surgery, with 16.7% occurring within 24 hours. The incidence of postoperative DVT in the intervention group (9.6%, 24/251) was significantly lower than that in the control group (22.9%, 58/253, P<0.001). Intraoperative IPC reduced the risk of DVT by 64.6% (0.354, 95% CI, 0.223-0.564), P<0.001). There was no significant difference in coagulation and platelet function between the two groups (all P>0.05).
Conclusions: DVT may develop within 24 hours after the craniotomy. Intraoperative application of IPC reduces the incidence of postoperative DVT.
Metabonomics study on the effects of acupuncture and moxibustion in a rat knee osteoarthritis model
Authors: Li Yachen , Tan Qian , Liang Fangyuan , Li Jia , Cai Guowei , Li Jing 
Published: October 2023

Abstract
For elucidating effects of acupuncture and moxibustion on the metabolism of knee osteoarthritis (KOA) in rats, A method for metabolite analysis based on high throughput liquid chromatography-tandem mass spectrometry (LC-MS) was used to investigate overall changes in serum between groups. Rats were divided into four groups: control (C), model (M), KOA plus traditional acupuncture and moxibustion (T) and KOA plus syndrome differentiation acupuncture and moxibustion (S). The paw withdrawal threshold, open-field test and Hematoxylin and eosinstaining were applied to assess the transformations of knee hyperalgesia and histopathology. The serum metabolic fingerprints were obtained by using metabonomics. Orthogonal partial least squares-discriminant analysis was evaluated to highlight the differences and identify possible biomarkers. The changing trends of potential serum biomarkers and biological pathways of differential metabolites among different groups were analyzed. The results showed that acupuncture and moxibustion alleviate hyperalgesia and cartilage degeneration in KOA rats. A total of 11 metabolites were annotated between C and M. Among the 11 metabolites, the level of 5 metabolites were reversed in group S, including Glutathione, Ornithine, Alpha-dimorphecolic acid, Carnosine, Anserine, and group T only reversed the contents of 2 metabolites, including Aminoadipic acid and Carnosine. T versus M, beta-Alanine metabolism, Histidine metabolism, Tryptophan metabolism, mTOR signaling pathway, and Chagas disease were the affected pathways. Therefore, beta-Alanine metabolism, Histidine metabolism, Tryptophan metabolism were the main differential metabolic pathways after T treatment. In the comparison of the S and M groups, beta-Alanine metabolism, mTOR signaling pathway, D-arginine and D-ornithine metabolism, PPAR signaling pathway, and Chagas disease were the affected pathways. In conclusion, acupuncture and moxibustion can ameliorate the symptoms of KOA. Compared with traditional acupuncture and moxibustion, treatment based on syndrome differentiation obviously exerted an effect on KOA-induced metabolic alterations, which is related to the improvement of oxidative damage, inflammatory, cell autophagy, and energy metabolism.
Prevalence and factors associated with rota-virus diarrhea among children aged 3-24 months at a referral hospital in Uganda: A crossectional study
Authors: Laker Goretty , Nankunda Jolly , Melvis Bernis Maren , Kajoba Dickson , Nduwimana Martin , Kimera Joel , Odong Richard Justine , Edyedu Isaac 
Published: October 2023

Abstract
Introduction: Rota-virus, the most common cause of severe acute diarrhea in the world, has a significant morbidity and mortality in children under two years. The impact of rotavirus immunization in Uganda is not well established. This study aimed to determine the prevalence, severity of dehydration and factors associated with Rota-virus diarrhea among children aged 3 to 24 months at Fort Portal Regional Referral hospital.
Methods: This was a cross-sectional hospital-based study done between December 2022 and February 2023. Stool specimens were tested for rotavirus infection using rapid immunochromatographic assay test. Data was analysed using SPSS version 22 with logistic regression done to determine the factors.
Results: Out of 268 children with acute watery diarrhea, 133 (49.6%) were females, aged ≤ 12 months 177 (66.0%). Children who were positive for rotavirus infection were 42 (15.7%), majority of whom had some dehydration 28(66.7%). The factors that were significantly associated with Rota-virus diarrhea at multivariable level were; age less than 12 months (AOR = 8.866, CI = 1.549–50.738, P = 0.014), male gender (AOR = 0.083, CI = 0.019–0.356, P = 0.001), coming from a home with another person with diarrhea (AOR = 17.821, CI = 3.484–91.166, P = 0.001) or a home where the water source was a well (AOR = 50.170, CI = 4.401–71.966, P = 0.002).
Conclusion: The prevalence of Rota-virus diarrhea was three times less in the post Rota-virus vaccination period compared to pre-rota vaccination period. Majority of the participants with rotavirus diarrhea had some dehydration. There is need for provision of safe water sources to all homes. Surveillance to determine the cause of the non rota diarrhea should be done.
WITHDRAWN: Effect of unsaturated flow on the occurrence and movement of light nonaqueous phase fluids
Authors: Zhao Guizhang , Cheng Jiale , Li Leicheng , Liu Yu , Zhang Hongli , Li Hongliang , Zhang Hepeng 
Published: October 2023

Abstract
Contamination from light nonaqueous phase fluids (LNAPL) and their derivatives during mining, production, and transportation has become a concern. Scholars have extensively studied LNAPL contamination, but the role of water content variation on its migration process in the unsaturated zone has not been sufficiently researched. In this study, indoor simulated vertical, one-dimensional, multiphase flow soil column experiments were conducted on the sandy soil of the Zhengzhou reach of the Yellow River to analyze the effect of water content on the occurrence state and migration rate of LNAPL. The experimental results indicate: (1) The migration rate of LNAPL exhibits an L-shaped trend during subsurface imbibition and a nonlinear relationship with migration time, and the migration rate and migration time of surface infiltration have a linear growth relationship. (2) The adsorption rate of LNAPL is negatively correlated with water content and positively correlated with oil content in the homogeneous non-saturated state. As LNAPL moves from the aeration zone to the saturated zone, the relationship between oil content and water content is nonlinear. (3) When the water content of the test medium is below 14% and the oil content is below 11%, LNAPL appears in the aeration zone in a solid phase. As the water content increases, the adsorption rate of the oil phase gradually decreases and eventually reaches the oil saturation point. (4) When the water content of the medium exceeds 8%, over time, LNAPL will be subject to oil-water interfacial tension, and the rate of LNAPL movement first decreases and then increases, displaying nonlinear growth.
Evaluation of the Immunogenicity of a DNA Vaccine for Leishmania Major based on LACK antigen using Calcium Phosphate and Chitosan Adjuvants
Authors: Gharaei Abdolaziz , Rahdar Mahmoud , Jorjani Oghlniaz , Saberi Sedigheh , Beiromvand Molouk , Feiz-Haddad Mohammad Hossein 
Published: November 2023

Abstract
Leishmaniasis is a parasitic disease of global health concern, and an affordable and effective vaccine could be a valuable solution. The aim of this study was to evaluate the immunogenicity of a Leishmania major DNA vaccine based on LACK antigen using calcium phosphate and chitosan adjuvants nano particles. Seventy Balb/c female mice 4–6 weeks old and 20–22 g weight were selected and were divided into 5 groups [Each group 14 mice]. First Group received plasmid LACK vaccine [PCDN3 + LACK], second group received PCDN3 + LACK + Nano adjuvant phosphate Ca, third group received PCDN3 + LACK + Nano adjuvant chitosan, forth group received PBS as negative control and fifth group did not receive vaccine as positive control. The mice were vaccinated two times intramuscular injection with three weeks interval. The mice were challenged with wild type of Leishmania major promastigote via intra dermal injection into the basal of their tail three weeks after the last vaccination. Clinical sign and measuring the size of lesion were evaluated two times in every week. To assay immune system responses, IFN-gamma and IL4 were analysed using ELISA test after and before vaccination and challenging. The finding showed that PCDN3 + LACK + n anno chitosan, PCDN3 + LACK + nano calcium phosphate and PCDN3 + LACK [without adjuvant] groups had the highest increase in IFN- gamma titer and the greatest decrease of IL4 titer. The size of Leishmania lesion were decreased in vaccination groups and the best results were found in PCDN3 + LACK + nano chitosan at last week of challenging. Based on the results, vaccination with using of LACK antigen with nano calcium phosphate adjuvant and nano chitosan may be an effective approach for controlling cutaneous leishmaniosis in endemic region.
Correlation of gestational age and age at death in Sudden Infant Death Syndrome: another pointer to the role of critical developmental period?
Authors: Habich Malgorzata , Zielenkiewicz Piotr , Paczek Leszek , Szczesny Pawel 
Published: October 2023

Abstract

Background
Filiano and Kinney proposed a triple-risk model for the sudden infant death syndrome (SIDS) that involves the intersection of three risks: (1) a vulnerable infant, (2) a critical developmental period in homeostatic control, and (3) an exogenous stressor(s). The primary evidence for the role of a critical developmental period in SIDS etiology is the peak of cases around the third month of life. Independently, several studies pointed to correlation between gestational age and age at death in SIDS, but used that to assess the SIDS risk for preterm infants, ignoring further ramifications.
Methods
We did a detailed analysis of CDC data spanning over two decades (1983-2011). We focused not only on the correlation between two age variables (gestational and age at death), but also on the possibility of misdiagnosis. Also, we attempted to account for potential biases in the data induced by the ICD-9/ICD-190 transition or the “Back to Sleep” campaign.
Results
The peak of deaths in the third month of life, that was the main argument for the role or critical development period, wasn’t unique to SIDS. However, we confirmed almost linear and negative correlation between gestational age and the week of death due to SIDS. This pattern (slope of correlation < 0 and significance of correlation p < 0.05) is characteristic for SIDS among all diseases analyzed in the study.
Conclusions
We interpret the results as the evidence of the role of the critical development period in SIDS etiology. Possibly more attention in the future research should be put to theories that are based on homeostatic control
Comparison of the Accuracy and Precision in Digital Scans for Implant-Supported Maxillary Hybrid Prosthesis: In Vitro Study
Authors: Sultanoğlu Elifnur Güzelce , Eroğlu Büşra Keleş 
Published: October 2023

Abstract
Background
This in vitro study aimed to assess and compare the accuracy and precision of six intraoral scanners (IOS) in the All-on-4 treatment concept.
Methods
An edentulous maxillary model was utilized, with four implants placed at 12, 15, 22, and 25 teeth positions, simulating the All-on-4 approach using resin acrylic. Following the placement of scanbodies, each of the six intraoral scanners (Primescan, Trios 3, Trios 4, Trios 5, Virtuo Vivo, Medit i 700) performed eight scans of the model. An industrial scanner was employed for the control group. Trueness evaluation involved aligning the data from the six dental scanners with the reference model. Precision analysis encompassed aligning the data from all six scanners within their respective groups. Data alignment and comparison were executed using the CloudCompare software (v2.11.3, General Public License of Telecom ParisTech, Paris, France). Statistical scrutiny encompassed the Shapiro-Wilk, Levene's, and Games-Howell tests.
Results
Among the scanners, Primescan exhibited the highest trueness (35,75±26,08 μm ), while the Medit i700 demonstrated superior precision (0,163 μm).
Conclusion
IOS can be used to make dental impressions within the All-on-4 concept. More comprehensive and clinical studies are needed on this subject.
An Experimental Study on Strength and Some Durability Characteristics of Concrete With 60% Replacement of Recycled Aggregate Concrete With Nanosilica
Authors: kuppili Prudvi , MURTY DSR 
Published: October 2023

Abstract
Scarcity of good natural materials have been playing a key role in present environmental consideration The present study is about the properties of concrete with natural crushed aggregate (NAC) recycled aggregate (RA). In this work, two sets of concrete grade. First set is (M20,M25,M30 & M35 with 100% M-sand & 100% crushed aggregate & 1.5% of Nano-silica by cement weight) i.e natural aggregate concrete (NAC), Second set (M20,M25,M30 &M35 with 100% Manufactured-sand & 60% recycled aggregate & 1.5% of Nano-silica by cement weight) i.e recycled aggregate concrete (RAC) with w/c ratio of (0.35–0.55) and PPC were taken. Mechanical & Durability properties of recycled coarse aggregate were studied with NAC & RAC and were tested at 3,7,28 days of curing. Durability aspects such as rapid chloride penetration & water permeability & Sorptivity and chloride attack are tested after 28days of curing,. this combination of recycled aggregate with natural aggregate. In this work, 0% of recycled aggregate concrete i.e (natural aggregate concrete) has shown good performance in mechanical properties when compared to 60% replacement of aggregates. 60% replacements shown good performance in durability properties. M-sand showed a good influence in contribution of strength properties.
Addressing healthcare practitioner genetic knowledge, attitude and practices in Gauteng Province, South Africa
Authors: Walters Sarah , Aldous Colleen , Malherbe Helen 
Published: October 2023

Abstract
Genetic testing and services are crucial in modern healthcare. Genetic testing is increasingly important in the diagnosis and treatment of more common diseases as well as rare diseases. Primary healthcare practitioners (HCPs), including general practitioners (GPs), are often the first contact point for patients seeking genetic information. However, many HCPs lack adequate genetic expertise to assist patients with genetic-related health concerns. Currently, no data is available on the genetic knowledge, attitudes, and behaviours of HCPs in the Gauteng province of South Africa. We aimed to investigate HCPs’ knowledge, attitude and behaviours towards genetics and genetic testing in the Gauteng province of South Africa. A tailored pre-validated questionnaire was administered online and in-person to targeted HCPs in the private healthcare sector in Gauteng, South Africa, to investigate genetic knowledge, behaviours and attitudes towards genetics and genetic testing. Of the 61 HCPs who responded (16.3% response), 95% were trained at South African medical schools. GPs, pathologists, physiotherapists, and specialists answered the questionnaire. Self-reported genetics knowledge was poor/very poor for 70% of respondents, making them ill-prepared to cope with the anticipated increasing demand for genetic information in primary healthcare. However, 93% displayed appropriate behaviour to genetic scenarios presented and showed positive attitudes towards providing and referring to, genetic services. Nevertheless, access to specific genetic information and resources was limited. Eighty-seven percent of respondents were interested in further genetic education. HCPs are interested in and expressed positive behaviours and attitudes towards genetics and genetic services, but their current knowledge is insufficient to cope with the growing demand.