bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybrid Modeling of Natural Gas Allocation amid Imbalances: Insights from Policy Scenarios in Iran
Authors: Rajabi Sajad , Shahdani Mahdi Sadeghi , ZahediVafa Mohammadhadi , Godarzi Gholamreza 
Published: January 2025

Abstract

This study investigates the economic implications of natural gas imbalances in Iran and develops an optimal allocation mechanism to address shortages under various policy scenarios. Utilizing a hybrid approach that combines Input-Output (IO) analysis and Linear Programming (LP), this research evaluates the impact of gas shortages across 70 economic sectors based on Iran’s 2023 IO table. The study considers four strategic scenarios: prioritizing GDP-contributing sectors (S-GDP), employment-intensive sectors (S-EML), high-value-added sectors (S-VAD), and export-oriented industries (S-EXP). Each scenario models phased gas outages to minimize economic and social costs, providing quantitative insights into sectoral vulnerabilities and policy trade-offs. Results indicate that strategic allocation of gas shortages can significantly mitigate societal welfare losses, with the S-GDP and S-EXP scenarios minimizing macroeconomic impacts. At the same time, S-EML and S-VAD emphasize social stability and productivity. Policy recommendations underscore the importance of aligning gas allocation strategies with national economic priorities to sustain resilience amid energy imbalances. This framework serves as a decision-support tool for policymakers managing gas crises in resource-dependent economies.
JEL Classification: Q41, Q48, C61, C67, O13
Human densities, not pollution, affect urban coyote boldness and exploration
Authors: Estien Cesar O. , Stanton Lauren A. , Schell Christopher J. 
Published: January 2025

Abstract

Comparative studies show that urban coyotes behave differently from their rural counterparts. However, these studies often treat cities as homogeneous. Cities feature diverse pressures for wildlife, such as variable human densities and environmental hazards, two factors that are known to drive increased risk-taking. Thus, this heterogeneity creates a shifting landscape of risk, which may drive locally adapted behavioral strategies within cities. Yet, the influence of these urban pressures on coyote behavior is not well understood. To investigate this, we conducted novel object testing at 24 sites across gradients of human density and pollution. We recorded coyote detections and coyote behavioral responses to the novel object, focusing on time spent alert, time spent close, and total exploration. We found that coyote detections varied with both human density and pollution, with coyote detections being markedly lower in areas with high human density and high pollution. Coyote boldness (time spent alert and close) and exploration were uniformly associated with human density, with coyotes in human-dense displaying elevated boldness and heightened exploration. Our results suggest that urban heterogeneity in human density impacts apex predator behavior, potentially having downstream consequences on human-carnivore coexistence.
Autophagy: a novel pathway for inhibitor of apoptosis protein-like protein-2 promoting breast cancer cell proliferation
Authors: Liu Shi , Zhang Zhiliang , Xiang Siqi , Jiang Shuangyan , Cui Ruxia , Peng Hang , Xiang Mingjun , Li Yusheng 
Published: January 2025

Abstract

Inhibitors of apoptosis protein-like protein-2 (ILP-2), which is a high expression in breast cancer, can cause apoptosis deficiency to promote proliferation on breast cancer cells. It is unclear that whether ILP-2 can promote breast cancer cell growth by regulating cellular autophagy. This study was designed to check the change of autophagy level in breast cancer cells (MCF-7 and MX-1) by MDC (monodansyl- cadaverin) staining through RNA interfering ILP-2 expression after addition of rapamycin (Rapa) treatment, and the results showed that the number of autophagic vesicles decreased significantly after the interference with ILP-2. Western blot analysis showed that expression of ILP-2, ATG14 and Beclin1 was downregulated and the ILC3-II/LC3-I ratio decreased in MCF-7 and MX-1 cells, while expression of P62 thereafter increased knockdown of ILP-2. A series of experiments such as CCK-8(Cell Counting Kit-8)assays, scratch assays, AO-EB༈Acridine Orange(AO)/EB Double Stain Kit ༉double staining analysis and western blot showed that knockdown of ILP-2 resulted in down-regulation of autophagy, significant increase in caspase-9, 3 expression and downregulation of MMP-9 (matrix metalloproteinase − 9) expression, increased apoptosis rate and inhibition of cell migration ability. Co-immunoprecipitation verified the existence of an interaction between ILP-2 and PI3K. Further Western blot results showed that after interference with ILP-2 expression, cellular autophagy levels and PI3K and Akt protein, LC3-II/LC3-I and Bcl-2 and Beclin1 protein expressions were significantly decreased. However, P62 protein expression was significantly increased. The results suggest that the possible pathways of ILP-2 are based on PI3K/Akt, Bcl-2/Bax, caspase-mediated signaling pathway to activate autophagy to inhibit apoptosis and promote tumor cell growth.
Clinical factors associated with prognosis in patients with differentiated thyroid cancer with lung metastases treated with radioiodine therapy
Authors: Hasegawa Tomokazu , Tsuchiya Takaaki , Kitagawa Mio , Gocho Toshio , Someya Masanori 
Published: January 2025

Abstract

Purpose
This retrospective study aimed to identify clinical factors associated with prognosis and to develop a scoring system to predict treatment efficacy in patients with differentiated thyroid cancer (DTC) who developed lung metastases following radioiodine therapy (RAI).
Methods
Among 300 patients who received RAI at our institution between January 1, 2017, and December 31, 2022, 74 exhibited lung metastases. We constructed a scoring system based on age (< 55 years vs. ≥55 years), the size of lung metastases (< 10 mm vs. ≥10 mm), the total diameter of lung metastases (≤ 20 mm vs. >20 mm), the presence or absence of iodine-131 (131I) uptake, and the pretreatment serum thyroglobulin (Tg) level (< 100 vs. ≥100 ng/mL). Each factor was assigned a specific point value, yielding a total possible score ranging from 0 to 7.
Results
The median progression-free survival (PFS) for all patients was 46.3 months, and the median overall survival (OS) was 76.0 months. Among these patients, 20 had 131I -avid lung metastases. In univariate analyses, age, size of lung metastases, total diameter of lung metastases, pretreatment Tg level, and 131I uptake were significantly associated with PFS. In multivariate analyses, the size of lung metastases and the presence or absence of 131I uptake were identified as independent prognostic factors. Based on the scoring system, patients were categorized into low-risk (0–2 points), intermediate-risk (3–4 points), and high-risk (5–7 points) groups. Both PFS and OS were significantly shorter in the high-risk group.
Conclusion
The scoring system developed in this study may serve as a valuable tool to guide treatment decisions based on risk stratification.
Transcriptome profiling uncovered the therapeutic potentials of proso millet malt in regulating hyperlipidemia
Authors: Pujari Nivedita , Hoskeri Joy 
Published: January 2025

Abstract

Adipose tissue dysfunction and adipogenesis are the pivotal events that are intricately linked to lipid disorders, notably encompassing hyperlipidemia and obesity. Here, we investigated transcriptome alterations in the visceral adipose tissue of male Wistar albino rats fed a high-fat diet, following the treatment with proso millet malt extract, through RNA sequencing technology. In-vivo anti-hyperlipidemic studies showed increased weight gain in high fat diet fed animals (130.67 ± 22.7g), whereas proso-millet fed animals showed subsequent decrease in body weight (67.67 ± 15.2g). The millet treatment also demonstrated significant improvement in lipid profile and liver function. Histopathological studies of adipose tissue revealed hypertrophy and hyperplasia conditions elevated in high fat fed animals was mitigated in animals fed with proso millet malt. Comprehensive transcriptomic profiling of HFD adipose tissues projected 8030 differentially expressed genes out of which 859 genes were significantly down-regulated in the adipose tissue transcriptome of proso millet-treated rats. Gene ontology analysis showed the involvement of genes in some of the prominent adipogenesis and adipocyte dysfunction-associated signaling pathways. Most of the genes involved in the PI3/AKT signaling pathway were found to be significantly down-regulated in proso millet-treated rats. Further disease pathway analysis showed Fos and Mapk genes to be the bottleneck proteins that play a major role in the Il-6 signaling pathway. Our findings provide compelling evidence that proso millet malt has significant potential for managing lipid disorders. This is achieved through its modulation of Fos and MAPK proteins within the PI3/AKT signaling pathway, which is crucial for adipogenesis. This suggests a novel avenue for therapeutic intervention in lipid disorders. We present one of the first studies focusing on the alteration of gene expression profiles in visceral adipose tissue induced by proso millet malt.
High frequency of muscle activity relapses in anti-synthetase syndrome
Authors: Choma Kaioan , Brasileiro Caio Gabriel Jeronymo Lima , De Souza Fernando Henrique Carlos , Miossi Renata , Shinjo Samuel Katsuyuki 
Published: January 2025

Abstract

Background
To date, no study has analyzed muscular activity relapses in anti-synthetase syndrome (ASyS), underscoring the necessity for this investigation.
Methods. This single-center retrospective cohort study, conducted between 2010 and 2014, included patients with anti-Jo-1 ASyS.
Results. A total of 95 patients with ASyS were identified. However, we excluded patients with anti-aminoacyl-tRNA-synthetase non-Jo-1 antibodies (n = 26), or myositis-associated autoantibodies (n = 25), without muscle involvement (n = 10), and those with incomplete data (n = 2). Consequently, a total of 48 anti-Jo-1 ASyS patients were evaluated with a median age of 44 (interquartile range: 35–54) years, predominantly female (81.3%), and of white ethnicity (79.2%). The median follow-up period was 80 (26–126) months. Twenty patients (41.7%) experienced second muscle activity during follow-up, whereas 10 (20.8%) and four (10.4%) experienced third and fourth muscle relapses, respectively. Compared to those without muscle relapse (n = 20), patients with at least one muscle relapse (n = 28) were younger (48 vs. 39 years, respectively; P = 0.020), exhibited a higher median creatine phosphokinase level (4144 vs. 8124U/L; P = 0.019), and demonstrated lower rates of pulmonary involvement, specifically ground-glass opacity (P = 0.006) and lung fibrosis (P = 0.003). There was no significant difference in treatment between patients with and without muscle relapse, except for cyclophosphamide, which was more frequently utilized in patients without muscle relapse (5.0% vs. 32.9%; P = 0.031).
Conclusion. A high frequency of muscle activity relapse was observed in patients with ASyS, particularly among younger individuals with high initial creatine phosphokinase levels and reduced pulmonary involvement. A small subset of patients experienced more than three muscle relapses.
Rhizobacteria from vineyard and commercial mycorrhizal fungi induce synergistic microbiome shifts within grapevine root systems
Authors: Darriaut Romain , Lailheugue Vincent , Wastin Jules , Tran Joseph , Martins Guilherme , Ballestra Patricia , Masneuf-Pomarède Isabelle , Ollat Nathalie , Lauvergeat Virginie 
Published: January 2025

Abstract

Background
The addition of bacteria and arbuscular mycorrhizal fungi (AMF) is a strategy used to protect plants against disease and improve their growth and yield, known as biocontrol and biostimulation, respectively. In viticulture, the plant growth promotion (PGP) potential of bacteria endemic to vineyard soil has been underexplored. Furthermore, most research about microbial biostimulants focuses on the effect on the plant, but little is known on how their application modify the soil and root microbial ecosystem, which may have an impact on plant growth and resistance. The objectives of this work were 1) to identify bacteria present in vineyard soils with functional PGP traits, 2) to test their PGP activity on young grapevines, in combination with AMF, 3) to assess the impact on the microbial communities and their inferred functions in the rhizosphere and plant roots.
Results
Two hundred bacteria were isolated from vineyards and characterized for their biochemical PGP activities. The most efficient were tested in vitro, both singly and in combination, on Lepidium sativum and grapevine plantlets. Two Pseudomonas species particularly increased in vitro growth and were selected for further testing, with and without two Glomus species, on grapevines planted in soil experiencing microbial dysbiosis in a greenhouse setting. After five months of growth, the co-application of PGP rhizobacteria and AMF significantly enhanced root biomass and increased the abundance of potentially beneficial bacterial genera in the roots, compared to untreated conditions and single inoculum treatments. Additionally, the prevalence of Botrytis cinerea, associated with grapevine diseases, decreased in the root endosphere. The combined inoculation of bacteria and AMF resulted in a more complex bacterial network with higher metabolic functionality than single inoculation treatments.
Conclusions
To our knowledge, this is the first study to examine and apply bacterial strains derived from soils of the same vineyard plot in co-application with commercialized fungi. The results show a remodeling of microbial communities and their functions associated with a beneficial effect on the plant in terms of growth and presence of pathogens. The observed synergistic effect of bacteria and arbuscular mycorrhizal fungi indicates that it is important to consider the combined effects of individuals from synthetic communities applied in the field.
An Advanced Cognitive Strategy for Drug Formulation Utilizing Deep Neural Networks and Feature-based Fusion
Authors: Singh Jagendra , Pal Yogesh , Suryavanshi Madhuri , S Jailani. , Amer A beer A. , Kumar Rakesh 
Published: January 2025

Abstract

This research introduces an innovative approach to drug formulation optimization by integrating a deep neural network (DNN) model with feature-based fusion. The study leverages diverse datasets, including molecular databases, biological response datasets, and pharmacokinetic information, to develop a holistic understanding of the intricate factors influencing drug behavior within biological systems. The DNN model, chosen for its capacity to handle high-dimensional data and capture complex relationships, was complemented by feature-based fusion to enhance the cognitive strategy for drug formulation. The quantitative evaluations of the models, conducted over 10 trials, yielded promising results. The DNN model demonstrated commendable performance with an average accuracy of 91.8%, precision of 89.2%, recall of 93.5%, and F1-score of 91.3%. However, the Feature-based Fusion approach consistently outperformed the DNN, achieving an average accuracy of 93.5%, precision of 91.7%, recall of 94.6%, and F1-score of 92.8%. These results highlight the superiority of the Feature-based Fusion approach in optimizing drug formulations, showcasing higher quantitative metrics and a more balanced trade-off between precision and recall. This research advances the field of drug formulation by providing a robust framework that integrates advanced cognitive strategies, contributing to more efficient and personalized therapeutic interventions.
Effect of Self-Regulated Learning by Nursing Students on Ethical Problems in Clinical Teaching
Authors: Bozkurt Ramazan , Dogu Ozlem 
Published: January 2025

Abstract

Background
The clinical education process, which is essential for nursing education, must be carried out by contributing to students' ethical values and self-regulated learning situations.
Aim
The study was conducted to evaluate the relationship between self-regulated learning situations and the ethical problems experienced by nursing students in clinical teaching and to determine their views.
Methods
This study used a mixed-method design with 371 nursing students. Data were collected using the Socio-Demographic Data Form, the Self-Regulated Learning Scale in Clinical Nursing Practice, and The Scale of Ethical Problems in Clinical Teaching in Nursing. The qualitative part was completed with 67 students who reported ethical issues.
Results
There was a weak, positive, and significant relationship between self-regulated learning in clinical practice and ethical problems in clinical teaching. Qualitative data were analyzed in two themes: "Team members with ethical problems" and "Ethical violation principles".
Conclusions
It was determined that nursing students experienced fewer ethical problems in clinical teaching as their self-regulated learning level increased. They also reported ethical problems within the team and that the most common violations were patient autonomy and respect for the individual.
Premarital spinal muscular atrophy screening program results from a province in the Black Sea region of Turkiye: Two years of experience
Authors: Yenercag Feyza Nur Topcu , Tarhan Günay Kaya 
Published: January 2025

Abstract

Objective
Spinal muscular atrophy (SMA) is a life-shortening autosomal recessive neuromuscular disease. In this study, the aim was to determine the use of premarital SMA carrier screening and the frequency of SMA carriers in this population.
Study Design:
The Premarital National SMA Carrier Screening Program was applied to 19,988 people between 27 December 2021 and 31 May 2024.
Result
A total of 453 people were found to be SMA carriers with heterozygote deletions in exon 7 of SMN1, indicating a carrier prevalence of approximately 1:44 (2.26%) in the population. After detailed genetic counseling, 441 partners were tested, and 13 were identified as SMA carriers. These couples were given information about preimplantation genetic diagnosis, and the birth of infants affected by SMA disease was prevented.
Conclusion
In our experience with carrier screening, awareness of premarital SMA carrier screening can be effectively maximized.
Experimental Design and Kinetics Study of Epoxidized Used Cooking Oil Using R
Authors: Bourkaib Nor El houda , irinislimane Ratiba , Belhaneche naima 
Published: January 2025

Abstract

The aim of this study is to optimize the parameters for epoxidizing used cooking oil (UCO) using in-situ generated performic acid (PFA), with a focus on employing the R Project for Statistical Computing. This optimization was achieved through a thorough full factorial experimental design, involving the variation of key parameters within defined ranges. Specifically, we varied the molar ratio of double bond C = C, hydrogen peroxide H2O2 and formic acid FA as follows: C = C/H2O2/FA from 1/1.5/1 to 1/2.5/1.5. Additionally, we considered temperature variations ranging from 40°C to 60°C, and adjusted the reaction times between 3 and 5 h. Following meticulous experimentation and analysis, we determined the optimal conditions to be a C = C/H2O2/FA molar ratio of 1/2.7/0.8, a temperature of 60°C, and a reaction time of 3 h. These ideal conditions served as the basis for a detailed study of reaction kinetics. Subsequent calculation of the activation energy resulted in an approximate value of 14.7 kcal mol-1. This finding strongly substantiates the possibility of replacing epoxidized fresh oil with epoxidized used cooking oil in industrial processes, underlining the potential for the effective utilization of recycled resources.
Drug resistance in bacteria: An analysis of the knowledge, attitudes and beliefs of primary care patients in areas that are key for better management of the problem
Authors: Handke Wiktoria , Karasiewicz Monika , Chawłowska Ewelina 
Published: January 2025

Introduction: The growing issue of bacterial resistance to antibiotics is a global problem and requires urgent action, including at the stage of primary healthcare (PHC). Objective: The goal of the study was to assess patients’ knowledge of antibiotic-resistant bacteria and patients’ attitudes toward antibiotic therapy.
Material and method: The study, using the survey method by means of a self-developed questionnaire, was conducted among 110 residents of the Wielkopolskie province in 2022.
Results: As many as 76.4% of the respondents had not come across information about antibiotic resistance at any PHC facility. A high percentage of the respondents believed that antibiotics are effective against influenza (60%) and the common cold (31.8%). In total, 27.3% of the study group reported taking medication that they had left over after previous therapies. Only 50.9% of the respondents properly handled expired antibiotics. At least eight out of ten respondents had not encountered any educational campaigns on the issue. Demographic factors do not differentiate the responses to key questions on antibiotic protection.
Conclusions: The unsatisfactory knowledge in the patient population that frequently undergoes antibiotic therapy calls for more effective health education. The role of medical personnel and conditions in which educational campaigns are more accessible is crucial.
The Effect of Cinnamon-Based Cookies on Blood Glucose and Antioxidant Levels of ST-Induced Diabetic Rats
Authors: Verdini Liana , Setiawan Budi , Sulaeman Ahmad , Wibawan I Wayan Teguh 
Published: January 2025

Introduction:
Diabetes mellitus is a significant public health concern as its global prevalence
continues to rise. Non-pharmacological treatments may offer promising results for diabetic
patients. This study aimed to evaluate the effects of cinnamon-based cookies on blood glucose
and antioxidant levels in diabetic rats.

Methods:
A randomized block design was used, involving five groups: a non-diabetic group, a
positive control group receiving 0.6 mg/kg/day of glibenclamide, a negative control group receiving
aqua dest, a group receiving cinnamon extract cookies (0.2 g/kg), and a group receiving powdered
cinnamon cookies (0.6 g/kg). A total of 40 Sprague-Dawley rats were randomly divided into
these groups (n = 8 per group). Diabetes was induced using streptozotocin (50 mg/kg). The intervention
lasted for 21 days, during which body weight and blood glucose levels were monitored
on days 0, 3, 7, 14, and 21. At the end of the intervention, the rats were sacrificed, and blood
serum was collected via intracardiac puncture. Body weight was measured using a digital scale,
while blood glucose levels were determined using a glucometer. The serum was then analyzed for
SOD, MDA, and insulin levels using the ELISA method.

Results:
The study demonstrated that both types of cinnamon cookies significantly decreased
blood glucose levels by day 3, with the cinnamon powder cookies achieving levels comparable to
those of non-diabetic rats by day 7. Additionally, both types of cookies significantly improved
SOD and insulin levels and reduced MDA levels in the rats. However, the body weight of the diabetic
rats was not fully restored.

Conclusion:
These findings suggest that cinnamon could be utilized in food product development
for blood glucose control, with cinnamon powder potentially offering more favorable results.

conclusion:
These findings suggest that cinnamon could be utilized in food product development for blood glucose control, with cinnamon powder potentially offering more favorable results.