bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Graft Maturation by MRI in Anterior Cruciate Ligament Reconstruction With and Without Concomitant Anterolateral Ligament Reconstruction
Authors: Yau W.P. , Lin Wei 
Published: January 2024

Background: Single-bundle anterior cruciate ligament reconstruction (SB-ACLR) with concomitant anterolateral ligament reconstruction (ALLR) has been associated with better clinical results when compared with isolated SB-ACLR. However, it is not known whether the improved outcomes are the result of the influence of concomitant ALLR on ACL graft healing. Purpose/Hypothesis: The purpose of this study was to determine whether concomitant ALLR is associated with improved graft ligamentization after SB-ACLR. It was hypothesized that ALLR would not affect graft healing. Study Design: Cohort study; Level of evidence, 3. Methods: A 1 to 1 matching study was conducted on a consecutive series of 732 patients who underwent ACLR using a hamstring tendon autograft between 2007 and 2019. Patients were excluded if they had skeletal immaturity, inflammatory joint disease, multiple ligament reconstruction (other than ALLR), or a graft rupture. Patients with concomitant SB-ACLR and ALLR (SB-ACLR/ALLR) and isolated SB-ACLR were matched 1 to 1 based on age, sex, examination under anesthesia (EUA) grade 3 pivot shift, EUA grade 3 anterior drawer test, presence of graft impingement, sagittal graft angle, skeletal maturity, lack of generalized ligamentous laxity, and multiple ligamentous injury. A total of 40 matched pairs underwent postoperative magnetic resonance imaging (MRI) within the second year after surgery to assess graft ligamentization, which was measured by the signal-to-noise quotient (SNQ) of the ACL graft. Results: The mean follow-up period was 41 months, with a 2-year follow-up rate of 80% in the SB-ACLR/ALLR group and 98% in the isolated SB-ACLR group. The mean duration between the index operation and MRI was 16.4 ± 3.4 months. No significant difference was observed in the SNQ of the ACL graft between the SB-ACLR/ALLR and SB-ACLR groups (6.9 ± 4.6 vs 5.2 ± 4.8, respectively; P = .066). Conclusion: Study findings indicated that a concomitant ALLR at the time of hamstring tendon autograft ACLR did not affect graft healing as assessed by the SNQ of the ACL graft.
The benefit of favorable venous outflow profile is mediated through reduced microvascular dysfunction in acute ischemic stroke
Authors: Li Siyuan , Hong Lan , Yang Wenhao , Liu Xinyu , Zhang Yiran , Ling Yifeng , He Zhijiao , Wang Xinru , Yue Yunhua , Dong Qiang , Wang Feng , Cheng Xin , Xin Cheng , Feng Wang , Yunhua Yue , Ying Guo , Guozhi Lu , Jin Zhang , Ziran Wang , Xiaoyu Zhou , Danhong Wu , Qijin Zhai , Qi Fang , Ying Tang , Xin Zou , Bosheng Fan , Rongbo Qu 
Published: January 2024

Introductions: Venous outflow (VO) is emerging as a marker of microvascular integrity in acute ischemic stroke. Using hemorrhagic transformation (HT) and infarct growth as mediators, we tested whether a favorable VO profile benefited functional outcome by reducing consequences of microvascular dysfunction. Patients and methods: Patients receiving thrombectomy in three comprehensive stroke centers due to acute anterior circulation occlusion were included. VO was assessed semi-quantitatively by the opacification of ipsilateral vein of Labbé, Trolard and superficial middle cerebral vein. HT was graded on follow-up CT. Infarct growth volume (IGV) was the difference of final infarct volume and baseline core volume. The association of VO and functional independence (90-day modified Rankin Scale ⩽ 2) was examined by logistic regression. Mediation analysis was performed among VO, HT or IGV, and functional outcome in patients with or without recanalization, respectively. Results: In 242 patients analyzed, VO was strongly correlated with functional independence and VO ⩾ 4 was defined favorable. In 175 patients recanalized, favorable VO was associated with a reduced risk of HT (OR = 0.82, 95% CI 0.71–0.95, p = 0.008), which accounted for 13.1% of the association between VO and favorable outcome. In 67 patients without recanalization, favorable VO was associated with decreased IGV (β = −0.07, 95% CI −0.11 to −0.02, p = 0.007). The association of favorable VO and functional independence was no longer significant (aOR = 4.84, 95% CI 0.87–38.87, p = 0.089) after including IGV in the model, suggesting a complete mediation. Discussion and Conclusion: In patients with acute anterior large vessel occlusion, the clinical benefit of VO may be mediated through reduced microvascular dysfunction.
Salto Talaris Fixed-Bearing Total Ankle Arthroplasty: Long-Term Results at a Mean of 10.7 Years
Authors: Veale Matthew , Endo Atsushi , Veale Nicholas , Swanson Daniel , Mahendraraj Kuhan , Brzezinski Andrzej , Hofmann Kurt , Slovenkai Mark 
Published: January 2024

Background: Total ankle arthroplasty (TAA) has become increasingly popular in the treatment for end-stage ankle arthritis in recent decades. However, there is limited evidence regarding the long-term clinical outcomes and complication rates of modern TAA implants. Methods: This study presents a follow-up on a previous cohort involving 78 patients (81 ankles) who underwent Salto Talaris fixed-bearing TAA to treat end-stage arthritis, with a mean postoperative follow-up of 5.2 years. The aim of this follow-up study was to assess the radiographic (33 patients, 35 ankles) and clinical (48 patients, 50 ankles) results from the original cohort at a mean of 10.7 years (range, 7.8-14 years). Results: At a mean of 10.7 years, the Kaplan-Meier estimated survivorship was 84.2% (95% CI, 71.9%-98.6%). For the patients reviewed, we did not find any change in patient-reported outcomes between an average 5- and 11-year follow-up. Measured total range of motion and plantarflexion did not change between 1 and 11 years, but dorsiflexion was measured as decreasing by an average of 4 degrees ( P < .02). Conclusion: In this longer-term follow-up of a limited cohort, we found that Salto Talaris fixed-bearing TAA demonstrated good long-term survival with relatively low rates of revision or other complications. Patient-reported outcome and range of motion measures revealed good stability. Level of Evidence: Level III, therapeutic.
An Exploratory Study Investigating the Correlates of Sexual Health Anxiety and Preferences for Digital Services Amongst Internet Users
Authors: Arneaud Gervan , Buchanan Tom , Gautier Chantal , Nadarzynski Tom 
Published: January 2024

Background: Sexual health clinics are under increasing pressure because of the demand for sexually transmitted infection (STI) screening services. Sexual health anxiety has been associated with increased clinic attendance and repeat screening. Aims: This study aimed to investigate the level of sexual health anxiety and its correlates in adults in England and their opinions on digital sexual health services. Setting and Design: An online cross-sectional study was conducted between May 2019 and January 2020 among internet users living in England aged 18 years and older. Methods: Participants were recruited from advertisements on social media (eg, Facebook, Twitter, Tumblr, and Reddit), and data were collected via an online Qualtrics survey. Statistical Analysis: Multiple regression analyses were used to identify correlates of sexual health anxiety. Results: One-hundred and thirty-eight participants completed the survey (mean age = 29.25, SD = 11.29; 51% male, 81% White, and 56% educated to the degree level). Average sexual health anxiety was mild ( M = 2.19, SD = .79), and the average subscale values ranged from 1.88 (SD = .92) to 2.67 (SD = 1.25). Higher sexual health anxiety was predicted by a higher frequency of obsessive-compulsive behavior and a greater risk perception of STIs. There were some overlaps between the predictors of subtypes, but further research is needed. Conclusion: Despite the levels of sexual health anxiety in the sample, the acceptability of digital sexual health services was high, but preferences leaned heavily toward face-to-face and in-person interaction.
Prevalence and Patterns of Sexual Dysfunction in Male Patients Suffering from Major Depressive Disorder Attending Psychiatry Outpatient Department
Authors: Jani Arpit , Chandrani Kalpesh , Prajapati Nisha 
Published: January 2024

Background: Major depressive disorder (MDD) can cause sexual dysfunction (SD), and it is more frequent with antidepressant treatment. Different antidepressant agents have different effects on SD according to their group and dose, which is studied in the present research. Aims: (a) To study the prevalence and patterns of SD in male patients with MDD, both drug naïve and on antidepressant treatment. (b) To correlate the severity of depression and SD. Settings and Design: Cross-sectional study carried out among 200 patients who attended to the psychiatry outpatient department of a tertiary care hospital. Materials and Methods: Every fifth patient with MDD attending to an investigator had been enrolled for the study. Sociodemographic details and clinical variables were obtained. The diagnosis of MDD established by the Diagnostic and Statistical Manual of Mental Disorders, fifth edition, criteria and the Hamilton Depression Rating Scale was used to assess the severity of depression. The Arizona Sexual Experience Scale and Change in Sexual Functioning Questionnaire-14 was applied to measure SD. Statistical Analysis: Statistical analysis was done using the χ2 test, unpaired t test, Pearson correlation coefficient, and analysis of variance test. Results: Total 82 (41%) patients had SD. Amongst them, 37 (45.12%) patients had the onset of SD before and 45 (54.88%) patients had the onset of SD after treatment. There was a statistically significant correlation between the severity of depression and SD ( P = .001), SD responses of patients on the selective serotonin reuptake inhibitor and tricyclic antidepressant ( P = .005). Sexual pleasure shows statistical significance with severity ( P = .004) and duration of depressive illness ( P = .03). The age factor was statistically significant with SD. Conclusions: Sexual dysfunction had significance with the severity of depression. Sexual pleasure had significance with severity and duration of depression, and some SD domains had significance with age groups.
Initiation and Receipt of Medication for Opioid Use Disorder Among Adolescents and Young Adults in 4 State Medicaid Programs in 2018: Improving Medicaid Quality Metrics
Authors: Lynch Victoria , Clemans-Cope Lisa 
Published: January 2024

Background: Medications for opioid use disorder (MOUD) in youth can reduce harms but many youths do not receive MOUD. Improving quality metrics of MOUD among youth can advance interventions for youth with opioid use disorder (OUD). Methods: We relied on 2018 Medicaid claims data from California, Colorado, Massachusetts, and New Mexico. We calculated the percentage of youth with OUD included in the quality metric for initiation, and the percentage who initiated by state. We also calculated the percentage excluded from the quality metric for initiation because they have an existing episode of OUD care and their MOUD receipt. We compared the characteristics of those who initiated/received MOUD to those who did not and compared state estimates after adjusting for age and health conditions. Results: Estimates of initiation exclude about half of the youth with OUD because they were in an existing episode of OUD care and could not be observed initiating. Among youth in a new episode of OUD care, only about 1 in 4 initiated and state estimates varied from 18.9% to 40.1%. Among youth with an existing episode of OUD care, more than half received MOUD and state estimates ranged from 35.2% to 71.3%. Youth who initiated MOUD or received MOUD with an existing OUD had more severe OUD but fewer co-occurring substance use disorders or mental or physical health diagnoses. After adjusting for age and health conditions, MOUD still varied substantially across states. Conclusions: Most youth with a new OUD diagnosis do not initiate MOUD but more than half of the youth in an existing OUD diagnosis receive MOUD. MOUD quality metrics that are disaggregated, adjusted, and inclusive of youth in an existing episode of care provide additional insight into opportunities to better support youth who might choose MOUD. State differences should be further studied for insight into policies that may affect MOUD.
Smoking and home oxygen therapy: a review and consensus statement from a multidisciplinary Swedish taskforce
Authors: Ahmadi Zainab , Björk Joar , Gilljam Hans , Gogineni Madhuri , Gustafsson Torbjörn , Runold Michael , Ringbæk Thomas , Wahlberg Josefin , Wendel Lotta , Ekström Magnus 
Published: January 2024

Background:Home oxygen therapy (HOT) improves survival in patients with hypoxaemic chronic respiratory disease. Most patients evaluated for HOT are former or active smokers. Oxygen accelerates combustion and smoking may increase the risk of burn injuries and fire hazards; therefore, it is considered a contraindication for HOT in many countries. However, there is variability in the practices and policies regarding this matter. This multidisciplinary Swedish taskforce aimed to review the potential benefits and risks of smoking in relation to HOT, including medical, practical, legal and ethical considerations.Methods:The taskforce of the Swedish Respiratory Society comprises 15 members across respiratory medicine, nursing, medical law and ethics. HOT effectiveness and adverse risks related to smoking, as well as practical, legal and ethical considerations, were reviewed, resulting in five general questions and four PICO (population–intervention–comparator–outcome) questions. The strength of each recommendation was rated according to the GRADE (grading of recommendation assessment, development and evaluation) methodology.Results:General questions about the practical, legal and ethical aspects of HOT were discussed and summarised in the document. The PICO questions resulted in recommendations about assessment, management and follow-up of smoking when considering HOT, if HOT should be offered to people that meet the eligibility criteria but who continue to smoke, if a specific length of time of smoking cessation should be considered before assessing eligibility for HOT, and identification of areas for further research.Conclusions:Multiple factors need to be considered in the benefit/risk evaluation of HOT in active smokers. A systematic approach is suggested to guide healthcare professionals in evaluating HOT in relation to smoking.
BATF and BATF3 deficiency alters CD8+ effector/exhausted T cells balance in skin transplantation
Authors: Li Chenghao , Liu Zongtao , Wang Zihao , Yim Wai Yen , Huang Yajun , Chen Yuqi 
Published: January 2024

Abstract
Background
It is well-established that CD8+ T-cells play a critical role in graft rejection. The basic leucine zipper ATF-like transcription factor (BATF) and BATF3 are transcriptional factors expressed in T lymphocytes. Herein, we investigated the functions of BATF and BATF3 in the differentiation and exhaustion of CD8+ T cells following alloantigen activation.

Methods
Wild-type CD8+ T cells, BATF-deficient (Batf−/−) CD8+ T cells, and CD8+ T cells deficient in both BATF and BATF3 (Batf−/−Batf3−/−) were transferred to B6.Rag1−/− mice, which received skin allografts from BALB/c mice. Flow cytometry was conducted to investigate the number of CD8+ T cells and the percentage of effector subsets.

Results
BATF expression positively correlated with effector CD8+ T cell differentiation. BATF and BATF3 deficiency promoted skin allograft long-term survival and attenuated the CD8+ T cell allo-response and cytokine secretion. Finally, BATF and BATF3 deficiency prompted the generation of exhausted CD8+ T cells.

Conclusions
Overall, our findings provide preliminary evidence that both BATF and BATF3 deficiency influences the differentiation of effector CD8+ T cells and mediates the exhaustion of CD8+ T cells, prolonging transplant survival. Targeting BATF and BATF3 to inhibit CD8+ T cell function has huge prospects for application as a therapeutic approach to prevent transplant rejection.
Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress
Authors: Wang Zicheng , Chen Zirui , Wu Yuchen , Mu Meiqi , Jiang Jingwen , Nie Wanting , Zhao Siwen , Cui Guowen , Yin Xiujie 
Published: January 2024

Abstract
Background
The NAC TF family is widely involved in plant responses to various types of stress. Red clover (Trifolium pratense) is a high-quality legume, and the study of NAC genes in red clover has not been comprehensive. The aim of this study was to analyze the NAC gene family of red clover at the whole-genome level and explore its potential role in the Pb stress response.

Results
In this study, 72 TpNAC genes were identified from red clover; collinearity analysis showed that there were 5 pairs of large fragment replicators of TpNAC genes, and red clover was found to be closely related to Medicago truncatula. Interestingly, the TpNAC genes have more homologs in Arabidopsis thaliana than in soybean (Glycine max). There are many elements in the TpNAC genes promoters that respond to stress. Gene expression analysis showed that all the TpNAC genes responded to Pb stress. qRT-PCR showed that the expression levels of TpNAC29 and TpNAC42 were significantly decreased after Pb stress. Protein interaction network analysis showed that 21 TpNACs and 23 other genes participated in the interaction. In addition, the TpNAC proteins had three possible 3D structures, and the secondary structure of these proteins were mainly of other types. These results indicated that most TpNAC members were involved in the regulation of Pb stress in red clover.

Conclusion
These results suggest that most TpNAC members are involved in the regulation of Pb stress in red clover. TpNAC members play an important role in the response of red clover to Pb stress.
Transcriptomic profiling of different developmental stages reveals parasitic strategies of Wohlfahrtia magnifica, a myiasis-causing flesh fly
Authors: Jia Zhipeng , Hasi Surong , Zhan Deng , Vogl Claus , Burger Pamela A. 
Published: January 2024

Abstract
Background
Wohlfahrtia magnifica is an obligatory parasite that causes myiasis in several warm-blooded vertebrates. Adult females deposit the first-stage larvae directly onto wounds or natural body orifices (e.g., genitalia) of the host, from where they quickly colonize the host tissue and feed on it for development. The infestation of W. magnifica can lead to health issues, welfare concerns, and substantial economic losses. To date, little is known about the molecular mechanisms of the W. magnifica-causing myiasis.

Results
In this study, we collected parasitic-stage larvae of W. magnifica from wounds of naturally infested Bactrian camels, as well as pupae and adult flies reared in vitro from the wound-collected larvae, for investigating the gene expression profiles of the different developmental stages of W. magnifica, with a particular focus on examining gene families closely related to the parasitism of the wound-collected larvae. As key proteins related to the parasite-host interaction, 2049 excretory/secretory (ES) proteins were identified in W. magnifica through the integration of multiple bioinformatics approaches. Functional analysis indicates that these ES proteins are primarily involved in cuticle development, peptidase activity, immune response, and metabolic processes. The global investigation of gene expression at different developmental stages using pairwise comparisons and weighted correlation network analysis (WGCNA) showed that the upregulated genes during second-stage larvae were related to cuticle development, peptidase activity, and RNA transcription and translation; during third-stage larvae to peptidase inhibitor activity and nutrient reservoir activity; during pupae to cell and tissue morphogenesis and cell and tissue development; and during adult flies to signal perception, many of them involved in light perception, and adult behavior, e.g., feeding, mating, and locomotion. Specifically, the expression level analysis of the likely parasitism-related genes in parasitic wound-collected larvae revealed a significant upregulation of 88 peptidase genes (including 47 serine peptidase genes), 110 cuticle protein genes, and 21 heat shock protein (hsp) genes. Interestingly, the expression of 2 antimicrobial peptide (AMP) genes, including 1 defensin and 1 diptericin, was also upregulated in the parasitic larvae.

Conclusions
We identified ES proteins in W. magnifica and investigated their functional distribution. In addition, gene expression profiles at different developmental stages of W. magnifica were examined. Specifically, we focused on gene families closely related to parasitism of wound-collected larvae. These findings shed light on the molecular mechanisms underlying the life cycle of the myiasis-causing fly, especially during the parasitic larval stages, and provide guidance for the development of control measures against W. magnifica.
Profiling of T cell repertoire in peripheral blood of patients from type 2 diabetes with complication
Authors: Yin YongHui , Sheng YingLi , Gao Shuo , Zhang JinTao , Wang WenKuan , Liu YingJun , Xu TingTing , Zhang Yi 
Published: January 2024

Abstract
Purpose
More than 90% of patients with diabetes worldwide are type 2 diabetes (T2D), which is caused by insulin resistance or impaired producing insulin by pancreatic β cells. T2D and its complications, mainly large cardiovascular (LCV) and kidney (Ne) complications, are the major cause of death in diabetes patients. Recently, the dysregulation of peripheral T cell immune homeostasis was found in most T2D patients. However, the characteristics of T-cell receptors (TCR) remain largely unexplored in T2D patients.

Patients and methods
Here we investigated the TCR repertoire using high-throughput sequencing in peripheral blood collected from T2D patient with (8 LCV and 7 Ne) or without complications.

Results
Our analysis of TCR repertoires in peripheral blood samples showed that TCR profiles in T2D patients with complications tended to be single and specific compared to controls, according to the characteristics of TCR repertoire in V-J combination number, diversity, principal component analysis (PCA) and differential genes. And we identified some differentially expressed V-J gene segments and amino acid clonotypes, which had the potential to contribute to distinguishing T2D patient with or without complications. As the progression of the disease, we found that the profiling of TCR repertoire was also differential between T2D patients with LVD and Ne complications base on this pilot analysis.

Conclusion
This study demonstrated the protentional unique property of TCR repertoire in peripheral blood of T2D patient with and without complications, or T2D patients with LVD and Ne complications, which provided the possibility for future improvements in immune-related diagnosis and therapy for T2D complications.