bluuebunny/crossref_metadata_2025_split huggingface dataset:
Corneal topographic indices of Scheimpflug camera in type 2 diabetic and non‐diabetic elderly population
Authors: Yekta AbbasAli , Khabazkhoob Mehdi , Hashemi Hassan , Ostadimoghaddam Hadi , Yekta Reihaneh , Ehsaei Asieh , Yekta Yeganeh , Narooie Foroozan , Siyahkamari Masoud 
Published: January 2024

Aims/Purpose: To determine the corneal topographic indices of Scheimpflug camera in type 2 diabetic and non‐diabetic in elderly population of Tehran, Iran.Methods: A total of 1105 subjects were selected using random cluster sampling from Tehran, Iran and categorized into two groups including diabetics and non‐diabetics. The diabetic group had HbA1c levels ≥6.4% with no other systemic problems. The non‐diabetic participants had normal eye findings and no systemic diseases. The keratometry values, Q‐value, index of surface variance (ISV), index of vertical asymmetry (IVA), central keratoconus index (CKI), keratoconus index (KI), index of height asymmetry (IHA), index of height decentration (IHD), anterior average radius of curvature (ARC), posterior average radius of curvature (PRC), and pachymetric progression index (PPI) values were measured using the Pentacam AXL.Results: A total participants included 429 diabetic (38.46% male) and 676 non‐diabetic (38.76% male). Only PPIavg and PPImax were higher in the diabetics versus non‐diabetics (p = 0.019 and p = 0.010, respectively). The HbA1c level was 5.47 ± 0.34, 6.70 ± 0.17, and 8.72 ± 1.16 in the first, second, and third group, respectively. There was a significant difference in PPImax between the three groups (p = 0.036). There were significant differences only in IVA, CKI, PPIAvg, and PPImax between different stages of DR (p = 0.045, p = 0.005, p = 0.002, and p = 0.004, respectively). The diabetic subjects were divided into two groups based on the presence of DR. There was a significant difference in ISV, IVA, PPIavg, and PPImax between diabetic subjects with and without DR (p = 0.016, p = 0.022, p < 0.001, and p < 0.001, respectively).Conclusions: The results of this study showed that DM and DR change several topographic indices and the HbA1c level may affect PPImax. Therefore, special attention should be paid to these patients for different treatment strategies.
Mir‐205‐5p as a regulator agent in cell migration processes
Authors: Sanchez Maria Ybarra , Santos Miriam Martinez , Oltra Maria , Pelluz Javier Sancho , Barcia Jorge 
Published: January 2024

Aims/Purpose: Endothelial cell migration is enhanced in pathological processes of the eye involving high glucose, such as diabetic retinopathy (DR). The microRNA miR‐205‐5p is identified as an important regulator of vasculogenesis and could slow down cell migration. The aim of this study is to understand the role of mir‐205‐5p in endothelial cell proliferation under high glucose (HG) conditions.Methods: HUVEC were seeded at a density of 5.0 × 104 cells/cm2. After 48 h, a wound was produced by scratching cell monolayers with a pipette tip. The cells were treated with different conditioned mediums from ARPE‐19 cells: control media, control media and miR‐205‐5p mimic, HG media and HG media and miR‐205‐5p. Images were taken at 0, 4, 8 and 24 h. The gap distance was analysed with Image J (migration n = 4 and viability n = 6).Results: HUVEC cells treated with HG media from ARPE‐19 cells close the gap faster than HUVEC cells treated with ARPE‐19 control media or ARPE‐19 control media with mimic miR‐205‐5p. The administration of mimic miR‐205‐5p in HG media significantly reduce migration of HUVEC cells. Besides, the presence of HG for 5 days reduces ARPE‐19 cells viability however miR‐205‐5p mimic protect them, increasing cell viability in control.Conclusions: Angiogenesis is mostly due to an enhancement of endothelial cell migration. That seems to be related to a reduction of miR‐205‐5p, released from RPE cells. When a miR‐205‐5p mimic was added in the medium, cell migration slowed down.
The miRNA‐mRNA Interactome of human trabecular meshwork cells treated with TGF‐ beta1
Authors: Roodnat Anton , Callaghan Breedge , Doyle Chelsey , Atkinson Sarah , Willoughby Colin 
Published: January 2024

Aims/Purpose: Pseudoexfoliation glaucoma (XFG) is an aggressive form of secondary open angle glaucoma, associated with activation of the TGF‐β pathway by TGF‐β1. MicroRNAs are small non‐coding genes that regulate mRNA expression. To investigate the role of TGFβ1 in glaucomatous changes in the trabecular meshwork (TM) and to determine how mRNA expression may be regulated by microRNAs in this condition, we performed a bioinformatics analysis to determine the microRNA‐mRNA interactome.Methods: Primary human trabecular meshwork cells were obtained from normal donors (n = 4) and treated with TGF‐β1 at 5 ng/mL for 24 h. RNA was extracted and miRNA‐Seq and mRNA‐Seq performed. Differential mRNA and miRNA expression was determined using a bioinformatics pipeline consisting of FastQC, STAR, FeatureCounts, edgeR (miRNA) and DESeq2 (mRNA). Putative interactions between differentially expressed mRNAs and miRNAs, in which the direction of expression of the regulated mRNA was required to be opposite to that of the regulating miRNA, were determined using interaction databases miRTarBase, TarBase, TargetScan and miRWalk. To classify mRNA species by biological function and pathway, gene enrichment was performed using Enrichr.Results: The resulting microRNA‐mRNA interactome consisted of 1202 interactions. Some highly connected microRNAs were hsa‐let‐7e‐5p, hsa‐miR‐20a‐5p, hsa‐miR‐122‐5p, and hsa‐miR‐29c‐3p. Most genes in all enriched biological functions and pathways were indicated to be regulated by microRNAs. The sub‐interactomes of several specific XFG‐related enrichment terms such as oxidative stress, unfolded protein response, signal molecules and ECM remodelling were determined.Conclusions: This work presents a genome‐wide microRNA‐mRNA regulatory network for human TM cells treated with TGF‐β1 and may serve to generate unbiased hypotheses about regulatory functions of miRNAs found to be differentially expressed in XFG.
Towards the preclinical diagnosis of diabetic retinopathy and diabetic macular oedema using tear samples
Authors: Ituarte Víctor Alegre , Andrés‐Blasco Irene , Gallego‐Martínez Alex , Casaroli Ricardo , Di Lauro Salvatore , Galarreta‐Mira David , Zanón‐Moreno Vicente , Pinazo‐Duran Maria Dolores 
Published: January 2024

Aims/Purpose: A wide spectrum of molecular‐genetic events, including inflammation (INF), angiogenesis (ANG), apoptosis (AP) and the concurrence of miRNAs and target genes, are involved in diabetic retinopathy (DR) and diabetic macular oedema (DMO). To unravel the complex pathogenesis of DR and DMO, we focus on the microvascular endothelial dysfunction (MVED) pathophysiologic pathways.Methods: Volunteers of both sexes, aged 35–80 years were enrolled and distributed into type 2 diabetics (T2DM), with DR (+DRG; n = 35), without DR (−DRG; n = 35), with DMO (DMOG; n = 28), and controls (CG; n = 40). Sociodemographic and comorbid data, ophthalmologic parameters, and molecular‐genetic variables related to INF, ANG, and AP, assayed in tear samples, were recorded. Statistics were performed by the R Core Team (2022) program.Results: Most of our participants were middle‐aged (mean age was 58 ± 11 years). Differential expression profile in tears was found statistically significant for SOCS6 gene (involved in INF) target of hsa‐miR‐155‐5p, VEGF‐A gene (implicated in ANG) target of hsa‐miR‐15b‐5p, and the BCL2L2 gene (AP effector) target of hsa‐miR‐10a‐5p, in both the +DRG and DMOG, as compared to the −DRG and CG.Conclusions: We confirm that INF, ANG, and AP, regulated by the hsa‐miR‐155‐5p, hsa‐miR‐15b‐5p and hsa‐miR‐10a‐5p, and its respective target genes SOCS6, VEGF‐A, and BCL2L2, expressed in tears, are key players in the development of structural and functional chorioretinal changes associated with MVED in DR/DMO patients. We may propose the above insights as potential diagnostic‐prognostic biomarkers for better managing the diabetic eyes and vision.
Genetic landscape of inherited retinal dystrophies in a Welsh Tertiary Referral Centre
Authors: Sanders Francis , Votruba Marcela 
Published: January 2024

Aims/Purpose: Inherited retinal dystrophies (IRD) are a group of predominantly monogenic disorders which have genetically heterogeneous origins and display wide clinical phenotypic heterogeneity. The current study aims to describe the clinical diagnoses, demographics, and genetic aetiology of patients in an adult cohort of Welsh patients to provide insight into the rates of genetic diagnosis of IRD. This will help inform patient prognosis and counselling in future clinical encounters.Methods: A comprehensive database of patients attending the tertiary referral clinic from 2011 to 2022 was compiled contemporaneously. Demographic data were collated from patients' clinical records. Subjects underwent genetic screening, including targeted gene sequencing, next‐generation sequencing‐based gene panel, or whole exome sequencing, to investigate the causative mutations.Results: A total of 365 probands (191 men and 174 women). The median age at which the patients developed visual symptoms related to IRD was 42 ± 17.7 years (range, 1–86 years). Retinitis pigmentosa (176; 48.2%), Macular Dystrophy (49; 13.4%), and Cone Rod Dystrophy (41; 11.2%) were the most common clinical diagnoses in this cohort. Among all the subjects, a genetic mutation was identified in 193 (52.9%) patients and the genetic aetiology of diseases was confirmed in 147 (40.3%) patients. Pathogenic mutations were identified in 48 retinal genes with the most commonly affected genes being ABCA4, USH2A and GUCY2D with 78, 44 and 13 mutations identified overall.Conclusions: The establishment of a molecular genetic diagnosis in this group of patients serves as a basis for genetic counselling and will allow patients to enrol in current and future gene‐based clinical trials, and benefit from any future gene‐based treatments.
Needle track technique in strabismus surgery
Authors: Bandhu Seema , Bansal Yashi , Bains Barinder , Moudgil Tania 
Published: January 2024

Aims/Purpose: Scleral perforation during the passage of suture needle is a known complication of strabismus surgery.[1–4] The present study was conducted as a pilot study to evolve a safe and easy technique of passing the suture needle through the sclera. A scleral tunnel was created with a 26‐guage needle through which the suture needle was passed.Methods: After taking clearance from the Institutional Ethics Committee, an interventional study was carried out on twenty consenting patients. A tunnel through approximately half the thickness of the sclera was created with the help of a bent 26‐gauge needle. The suture needle was then passed through this tunnel. Rest of the steps of the surgery were carried out in the conventional manner. In a control group of same number of patients, the surgery was performed in the conventional method. Both the groups were followed up for any complications. Ease of passing suture and surgeons' satisfaction with the technique was noted on a 5‐point Likert scale.Results: None of the patients in either group suffered from any complications. Four surgeons participated in the study. The average Likert scale score of the surgeons for satisfaction with the technique was 5 on a scale of 5. The score for ease of passage of suture needle was 5 on a score of 5.Conclusions: The needle track is an easy technique to perform which eases the passage of suture needle through the sclera. This will increase the confidence of Ophthalmologists, specially the residents in performing strabismus surgery.References1. Simon JW. Complications of strabismus surgery. Curr Opin Ophthalmol 2010;21:361–6.2. Dang Y, Racu C, Isenberg SJ. Scleral penetrations and perforations in strabismus surgery and associated risk factors. J AAPOS. 2004;8:325–31.3. Morris RJ, Rosen PH, Fells P. Incidence of inadvertent globe perforation during strabismus surgery. Br J Ophthalmol. 1990;74:490–3.4. Olitsky SE, Coats DK. Complications of Strabismus Surgery. Middle East Afr J Ophthalmol. 2015 Jul‐Sep;22(3):271–8. doi: 10.4103/0974‐9233.159692. PMID: 26180463; PMCID: PMC4502168.
Intravitreal bevacizumab in choroidal neovascularisation in Vogt‐Koyanagi‐Harada disease in a child: A review of literature
Authors: Chahir Roukaya , Ghizlane Daghouj , Mtalai Naoual , Elmaaloum Loubna , Allali Bouchra , Elkettani Asmaa 
Published: January 2024

Aims/Purpose: Interest of bevacizumab in children in the treatment of choroidal neoplasms in Vogt Koyanagi Harada syndrome.Methods: In this work we report the case of an extra‐foveal choroidal neovascularization in a child followed for VKH syndrome with a good response to intravitreal injections of bevacizumab.Results: We report the case of a 12‐year‐old child who initially presented to the hospital in 2020 for bilateral decreased visual acuity and headache. There was no history of systemic disease. Initial visual acuity was 1/10 in both eyes. Biomicroscopic examination revealed granulomatous retrodescemetic precipitates, 2× anterior chamber tyndall, 1× vitreous tyndall, and bilateral exudative retinal detachment associated with bilateral papilledema in both eyes. Based on these findings, a diagnosis of Vogt‐Koyanagi‐Harada syndrome was made, and treatment was initiated with intravenous, oral, and topical corticosteroids. Visual acuity improved to 6/10 OD and 7/10 OS the following month. The patient was then followed up at the university hospital regularly. After controlling the inflammation, steroids were decreased over a period of 2 years but the patient relapsed at high dose to declare cortico‐dependence. Anti‐TNF alpha was started to control the inflammation and visual acuity remained stable until July 2022. Choroidal neovascularization then developed, reducing the patient's visual acuity to 3/10 in both eyes within 1 month. Fluorescein angiography and macular OCT confirmed the diagnosis of extrafoveolar choroidal neovascularization in both eyes. After obtaining informed consent, intravitreal bevacizumab was injected 3 times at 6 week intervals in each eye. After the 6th injection, the patient's best‐corrected visual acuity improved to 8/10 in the right eye and 10/10 in the left eye. The fundus photograph illustrates the regression of the choroidal neovessels. Fluorescein angiography confirmed the reduction in leakage. Choroidal neovascular membranes can cause severe visual impairment and are a major challenge in the management of patients.Conclusions: This case illustrates that intravitreal bevacizumab has a possible role in the treatment of the disease, with regression of neovascular lesions in the eye.
Ptosis revealing breast carcinoma about a case
Authors: Okomiko Delphes Mathilde Otsasso , Hammoumi Zineb , Houti Sarah , Ghizlane Daghouj , El Maaloum Loubna , Allali Bouchra , El Kettani Asmaa 
Published: January 2024

Aims/Purpose: The purpose of our work is to describe the clinical and therapeutic aspects of a clinical case of ptosis revealing breast carcinoma.Methods: We report the case of a 61‐year‐old patient, with no pathological history, who presented to the ophthalmological emergency department for a unilateral left ptosis, of rapidly progressive onset.Results: This is a 61‐year‐old patient, with no pathological history, who presents to the ophthalmological emergency department for a unilateral left ptosis of rapidly progressive onset. Ophthalmological examination noted visual acuity at 10/10 in 2 eyes and Parinaud 2 in near vision in 2 eyes, complete ophthalmoplegia of oculomotor nerve (III) with severe ptosis on the left and relative left afferent pupillary deficit. The ophthalmological examination of the right eye was unremarkable. The general examination revealed a right breast mass. Cerebral angio‐MRI revealed four metastatic lesions on the left, above and under tentorial. A mammography showed a diffuse invasive lesion classified ACR 5 (American College of Radiology) on the right. A breast biopsy with histopathological study confirmed the diagnosis of poorly differentiated and invasive lobular carcinoma of the right breast. The patient is currently undergoing chemotherapy treatment.Conclusions: Acute ptosis is an emergency. Thus, a well‐conducted interrogation as well as a thorough general and ophthalmological examination make it possible to orient the etiological assessment and to take early and adequate management of a pathology that may be life‐threatening for the patient.References1. Barale PO, De Nicola R, Mann F, Miléa D, Tuil E. Ophtalmologie en Urgences. Elsevier Health 2018; 4e édition: p. 309.2. Audren F. Les paralysies de la IIIe paire crânienne: diagnostic. Pratiques en Ophtalmologie 2011; 5:119 A‐44.3. Birzu C, Royer‐Perron L, Hoang‐Xuan K. Tumeurs intracrâniennes. La Revue du Praticien 2020; 70(2): 39–46.
Evaluation of machine learning algorithms using OCT and OCT angiography for the diagnosis of multiple sclerosis
Authors: Lozano María Pilar Rojas , Mesa Pablo García , Gutiérrez Nuria Díaz , Valverde Juan José Gómez 
Published: January 2024

Aims/Purpose: We present a comprehensive evaluation of machine learning (ML) algorithms using Optical coherence tomography (OCT) and optical coherence tomography angiography (OCT‐A) for the diagnosis of Multiple sclerosis (MS).Methods: Prospective observational study using OCT volumes acquired with Cirrus HD‐OCT 5000 (Carl Zeiss, Meditec, Dublin, CA, EEUU) using the scanning protocols Optic Disc Cube 200 × 200 and Macular Cube 512 × 128, and OCT‐A macular volumes 175 × 350 with FOV 6 × 6 mm and OCT‐A of the Optic Nerve Head (ONH) with FOV 4.5 × 4.5 mm. The total data set consisted of 79 eyes of 40 patients with MS and 54 eyes of 27 control subjects. We evaluated classification algorithms such as Support Vector Machines, k‐Nearest Neighbour, Decision Trees, Random Forest, Extra Trees Classifier and Gaussian NB using data extracted from Ganglion Cell Analysis (8 parameters), Macular Thickness Analysis (7 parameters) and Optic Disc Analysis (5 parameters) from the OCT volumes and 5 parameters from the analysis of the macular OCT‐A volumes and 6 parameters from the analysis of the ONH. We studied all the ML algorithms considering three scenarios: OCT (20 parameters), OCT‐A (11 parameters), and OCT + OCT‐A (31 parameters). The models were trained using a 5‐fold cross‐validation strategy. We calculated the accuracy, F1‐score for each model and the area under the curve (AUC) was used to select the best ML algorithm. We applied SHAP (SHapley Additive exPlanations) to explain the output of the best ML model.Results: For the MS diagnosis, the best results were obtained for OCT with Gaussian NB (accuracy 0.865 ± 0.085, F1‐score 0.864 ± 0.086 and AUC 0.871 ± 0.081), for OCT‐A with Random Forest (accuracy 0.832 ± 0.032, F1‐score 0.832 ± 0.043 and AUC 0.831 ± 0.043) and for OCT + OCT‐A with Gaussian NB (accuracy 0.885 ± 0.028, F1‐score 0.886 ± 0.028 and AUC 0.891 ± 0.034). The input parameters with the highest contribution to the model's predictions were the minimum ganglion cell thickness and the temporal flow index in the ETDRS grid.Conclusions: This study highlights the efficacy of machine learning techniques in utilizing combined parameters from OCT and OCT‐A images of the macula and ONH tests to facilitate early diagnosis of MS. Moreover, it underscores the significance of ophthalmic examination in the comprehensive management of the disease.
Ocular tics in Paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections (PANDAS)
Authors: Dore Stefano , Zinellu Angelo , Satta Daniele , Boscia Giacomo , Murgia Carlo , Pinna Antonio 
Published: January 2024

Aims/Purpose: Little is known about ocular tics in Paediatric Autoimmune Neuropsychiatric Disorders associated with Streptococcal infections (PANDAS). The purpose of this retrospective study was to assess ocular symptoms in 40 children with PANDAS.Methods: The clinical records of children with PANDAS referred to the Ophthalmology Unit, Azienda Ospedaliero‐Universitaria di Sassari, Sassari, Italy, between 2010 and 2019 were examined. The detection of ocular tics was investigated. Data about plasma antistreptolysin O (ASO) and anti‐DNAse b titres, erythrocyte sedimentation rate (ESR), plasma C‐reactive protein (CRP), and antibiotic use were recorded and statistically analysed.Results: 40 children (34 boys, 6 girls; mean age: 7.6 ± 2.5 years) with PANDAS were identified; 33 (82.5%) showed ocular tics, whereas the remaining 7 (17.5%) had extraocular tics. Children with ocular tics had significantly higher plasma values of anti‐DNAse b antibodies (p = 0.04) and CRP (p = 0.016) than those with extraocular tics. A course of oral antibiotics was given to 25 (76%) out of 33 children with ocular tics. All the treated patients showed marked improvement/complete resolution of symptoms; conversely, no improvement was observed in those untreated. A post‐antibiotic recurrence occurred in 8 (32%) children.Conclusions: Results suggest that higher plasma values of anti‐DNAse b antibodies and CRP may play a role in the pathophysiological mechanisms underlying ocular tics in PANDAS. Oral antibiotics may be beneficial in improving ocular symptoms. Further research is necessary to confirm our findings.
Changes in the structure and function of the visual pathway in subjects at high risk for the development of Alzheimer's disease: 27 months of follow‐up
Authors: López‐Cuenca Inés , Sanchez‐Puebla Lidia , Gutierrez María Álvarez , Salobrar‐Garcia Elena , Elvira‐Hurtado Lorena , Barabash Ana , Ramirez‐Toraño Federico , Fernández‐Albarral José A. , Matamoros José A. , Salazar Juan Jose , Ramirez Jose Manuel , de Hoz Rosa 
Published: January 2024

Aims/Purpose: In addition to advanced age, two of the most important risk factors for the development of Alzheimer's disease are having a family history of the disease (FH+) and being a carrier of at least one Ɛ4+ allele for the ApoE gene (ApoE Ɛ4+). AD generates changes in the retina and visual function when the disease is established, so the aim of the present work is to know if these changes appear in healthy subjects but with high genetic risk to develop the disease and to analyse if these changes evolve along 27 months of follow‐up.Methods: A retinal thickness analysis by optical coherence tomography and visual function by assessing visual acuity (VA), contrast sensitivity (CS), colour perception, perception digital test and visual field analysis was completed by all participants. For structural findings analysis subjects were divided into (FH+ ApoE Ɛ4+) and (FH‐ ApoE Ɛ4‐) groups and for functional analysis these subgroups were subdivided by ages between 40 and 60 years and subjects over 60 years.Results: After 27 months of follow‐up, the FH+ ApoE Ɛ4+ group experienced changes in the thickness of all retinal layers except the outer layers. In this group, the inner nuclear layer showed progressing changes, but there was no progression in visual function. However, compared to the FH‐ ApoE Ɛ4‐ group at 27 months, the improvement in visual acuity and contrast sensitivity (3 and 12 cpd) observed during the initial visit was maintained.Conclusions: In cognitively healthy individuals at high risk of developing AD structural changes in the retina continue to progress after 27 months, while functional changes detected at the beginning remain stable.