bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optineurin provides a mitophagy contact site for TBK1 activation
Authors: Yamano Koji , Sawada Momoha , Kikuchi Reika , Nagataki Kafu , Kojima Waka , Endo Ryu , Kinefuchi Hiroki , Sugihara Atsushi , Fujino Tomoshige , Watanabe Aiko , Tanaka Keiji , Hayashi Gosuke , Murakami Hiroshi , Matsuda Noriyuki 
Published: January 2024

AbstractTank-binding kinase 1 (TBK1) is a Ser/Thr kinase that is involved in many intracellular processes, such as innate immunity, cell cycle, and apoptosis. TBK1 is also important for phosphorylating the autophagy adaptors that mediate the selective autophagic removal of damaged mitochondria. However, the mechanism by which PINK1-Parkin-mediated mitophagy activates TBK1 remains largely unknown. Here, we show that the autophagy adaptor optineurin (OPTN) provides a unique platform for TBK1 activation. Both the OPTN-ubiquitin and the OPTN-pre-autophagosomal structure (PAS) interaction axes facilitate assembly of the OPTN-TBK1 complex at a contact sites between damaged mitochondria and the autophagosome formation sites. At this assembly point, a positive feedback loop for TBK1 activation is initiated that accelerates hetero-autophosphorylation of the protein. Expression of monobodies engineered here to bind OPTN impaired OPTN accumulation at contact sites, as well as the subsequent activation of TBK1, thereby inhibiting mitochondrial degradation. Taken together, these data show that a positive and reciprocal relationship between OPTN and TBK1 initiates autophagosome biogenesis on damaged mitochondria.
Repurposing an endogenous degradation domain for antibody-mediated disposal of cell-surface proteins
Authors: Schmitt Janika , Poole Emma , Groves Ian , Owen David J , Graham Stephen C , Sinclair John , Kelly Bernard T 
Published: January 2024

AbstractThe exquisite specificity of antibodies can be harnessed to effect targeted degradation of membrane proteins. Here, we demonstrate targeted protein removal utilising a protein degradation domain derived from the endogenous human protein Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9). Recombinant antibodies genetically fused to this domain drive the degradation of membrane proteins that undergo constitutive internalisation and recycling, including the transferrin receptor and the human cytomegalovirus latency-associated protein US28. We term this approach PACTAC (PCSK9-Antibody Clearance-Targeting Chimeras).
Systematic identification of 20S proteasome substrates
Authors: Pepelnjak Monika , Rogawski Rivkah , Arkind Galina , Leushkin Yegor , Fainer Irit , Ben-Nissan Gili , Picotti Paola , Sharon Michal 
Published: January 2024

AbstractFor years, proteasomal degradation was predominantly attributed to the ubiquitin-26S proteasome pathway. However, it is now evident that the core 20S proteasome can independently target proteins for degradation. With approximately half of the cellular proteasomes comprising free 20S complexes, this degradation mechanism is not rare. Identifying 20S-specific substrates is challenging due to the dual-targeting of some proteins to either 20S or 26S proteasomes and the non-specificity of proteasome inhibitors. Consequently, knowledge of 20S proteasome substrates relies on limited hypothesis-driven studies. To comprehensively explore 20S proteasome substrates, we employed advanced mass spectrometry, along with biochemical and cellular analyses. This systematic approach revealed hundreds of 20S proteasome substrates, including proteins undergoing specific N- or C-terminal cleavage, possibly for regulation. Notably, these substrates were enriched in RNA- and DNA-binding proteins with intrinsically disordered regions, often found in the nucleus and stress granules. Under cellular stress, we observed reduced proteolytic activity in oxidized proteasomes, with oxidized protein substrates exhibiting higher structural disorder compared to unmodified proteins. Overall, our study illuminates the nature of 20S substrates, offering crucial insights into 20S proteasome biology.
A high sucrose detection threshold is associated with increased energy intake and improved post-prandial glucose response independent of the sweetness intensity of isocaloric sucrose solutions
Authors: Preinfalk Verena , Schweiger Kerstin , Hüller Leonie , Dunkel Andreas , Kimmeswenger Isabella , Deck Corinna M. , Rust Petra , Somoza Veronika , Krammer Gerhard E. , Ley Jakob P. , Lieder Barbara 
Published: January 2024

AbstractSeveral studies proposed a role for the sweet taste receptor in energy intake and blood glucose regulation, but little is yet known about the impact of the individual sweet taste perception. Here, we found in a cross-over human intervention study with 29 male participants that modulating the sweetness of an isocaloric sucrose solution did not influence postprandial plasma concentrations of blood glucose and associated hormones over 120 min and 2 h post-load energy intake. Independent of the sweetness of the test solution, tests persons with a higher sucrose detection threshold had an average of 402 ± 78.8 kcal (39 ± 21%) higher energy intake and a higher glucose/insulin ratio, combined with a higher liking for sweet tasting food, than the test persons of the low threshold group. The body composition suggested a higher fat-free mass in the high threshold group that may have influenced energy intake and post-prandial glucose responses.
Soft-templated, mesoporous Co3O4 thin films for electrocatalysis of the oxygen evolution reaction
Authors: Wu Qingyang , Mellin Maximilian , Lauterbach Stefan , Tian Chuanmu , Dietz Christian , Hofmann Jan P. , Einert Marcus 
Published: January 2024

Mesoporous Co3O4 thin films, prepared by dip-coating and using Pluronic® F-127 as template, demonstrated improved OER activity compared to dense Co3O4 by providing high surface areas with increased concentration of catalytically active surface sites.
A Brief Intervention to Teach Parents Naturalistic Language Facilitation Strategies
Authors: Harrington Emily K. , Hadley Pamela A. 
Published: January 2024

Purpose:
This proof of concept study assessed the feasibility of a novel approach to teaching parents naturalistic language facilitation strategies in a single session. We investigated whether parents could learn to use the See and Say Sequence, which integrated responsive and language modeling strategies and measured the impacts that this intervention had on features of their input.

Method:

Fourteen parent–child dyads participated in the study. Children ranged from 15 to 23 months of age and produced between three and 135 words. Five parents had concerns about their children's rate of language development. Parents were taught the See and Say Sequence during a brief single session (
M
= 18.98 min,
SD
= 2.65 min) using the Teach–Model–Coach–Review instructional process. We analyzed parents' use of the three See and Say Sequence components, total number of utterances, and mean turn length, as well as responsive and linguistic features of parent input before and after the brief intervention.

Results:
Following intervention, parents significantly increased their use of the three See and Say Sequence components and decreased their total number of utterances and mean turn length. In addition, the use of the See and Say Sequence components substantially altered the overall composition of parent input.

Conclusions:
The results of this preliminary study demonstrate the feasibility of the See and Say Sequence in teaching responsive and language modeling strategies in a single session. We discuss the potential use and future evaluation of the See and Say Sequence as an option for early intervention service delivery.
Narrative Discourse in Youth Offenders: Examining Individual Differences
Authors: Shirley Rhiannah , Silverman Claire , Tran Claire , Nippold Marilyn A. 
Published: January 2024

Purpose:
This study examined narrative discourse in youth offenders, focusing on quality of story retelling, story comprehension, critical thinking, and the use of complex syntax.

Method:

The participants were 15 incarcerated adolescents, ages 13–18 years (
M
age
= 16 years). Each was evaluated at their detention center via Zoom, using a standardized language test and language samples that elicited narrative speaking with fables. After retelling a fable, the participant answered questions that examined story comprehension and critical thinking.

Results:
Most participants performed below average on the standardized language test and had difficulty on one or more of the language sampling measures. Areas of concern included quality of story retelling, story comprehension, critical thinking, and the use of complex syntax. Had language sampling not been employed, those weaknesses might have been overlooked.

Conclusions:
When evaluating youth offenders, it is important to elicit language samples that can provide detailed information about an adolescent's ability to communicate for genuine purposes. In this regard, the tasks employed in the current study could be helpful to speech-language pathologists in working with youth offenders, enabling them to pinpoint deficits and offer targeted intervention.
Step‐by‐step image enhancement method for PTZ‐camera based crack detection in expressways
Authors: Xia Zhiqiang , Shao Chunyan , Feng Xuezhi , Wang Huaiqiang 
Published: January 2024

AbstractCracks on highway pavement surfaces are among the most critical problems affecting expressway maintenance. A pan/tilt/zoom (PTZ) camera‐based crack detection method was proposed, owing to the intelligent traffic system (ITS) development of an expressway network in China. However, the quality of the crack image decreased at distant PTZ camera distances, resulting in a low mean average precision (MVP) for crack detection, which is difficult to implement in civil engineering. A weighted superposition‐based crack enhancement algorithm for crack detection was proposed to enhance crack images collected by PTZ cameras. Compared with the poor generalization ability of using a single image enhancement method, this method combines the advantages and characteristics of multiple image enhancement methods, thereby ensuring the quality of crack images. This enhances the visual characteristics of the cracks to adapt to various illuminations, thereby improving the crack detection rate and providing scientific and technological reserves for its application in highway maintenance engineering. Further comparison tests on crack detection for pavement crack images collected from the China G4/Jingshi Highway demonstrated that the proposed method was efficient in detecting is efficiently to detect cracks, achieving 96% MAP and 94% F1 values after enhancing the crack images, thereby providing an objective and promising means of crack detection using PTZ cameras.
Assessment on gas‐polyethylene terephthalate solid interface partial discharge properties of C4F7N/CO2 gas mixture for eco‐friendly gas insulating transformer
Authors: Xiao Song , Wang Yifan , Ren Chenhua , Xia Haoran , Zhao Yue , Qin Jingzi , Zhang Xiaoxing , Luo Yi , Li Yi 
Published: January 2024

AbstractThe eco‐friendly insulating gas perfluoroisobutyronitrile (C4F7N) is potentially used in gas‐insulated transformers (GIT) to replace sulphur hexafluoride (SF6). However, evaluation of the long‐term insulation reliability and gas–solid interface discharge decomposition characteristics of the gas–solid film insulation structure in GIT is indispensable. The authors simulated the gas–solid film insulation structure in GIT and explored the interface partial discharge (PD) characteristics of C4F7N/CO2 gas mixture with polyethylene terephthalate (PET). The effect of gas pressure, mixing ratio on gas–solid interface gas decomposition, PET degradation was investigated, and the interaction mechanism was analysed. It is found that the interface PD generated three degradation regions on a PET film. The gas–solid interface reaction in the electrode contact region and the discharge development trace was significantly higher than that of halation region. The content of gas decomposition products decreases with the increase of gas pressure and the PD intensity of SF6‐PET is inferior to that of C4F7N/CO2 under the same condition. Relevant results provide reference for the development and application of C4F7N/CO2 based GIT.
Star formation in G11.497-1.485: Two-epoch VLA study of a 6.7 GHz methanol maser flare
Authors: Bayandina O. S. , Burns R. A. , Caratti o Garatti A. , Kurtz S. E. , Moscadelli L. , Shakhvorostova N. N. , Sobolev A. M. , Tanabe Y. , Val’tts I. E. , Yonekura Y. 
Published: January 2024

Context. Maser flares are particularly significant in the study of massive star formation as they not only signal but also provide unique insights into transient phenomena such as accretion bursts.
Aims. With this project, we aim to investigate the context of the ongoing 6.7 GHz methanol maser flare in the little-known massive star-forming region G11.497-1.485.
Methods We carried out two epochs of the Karl G. Jansky Very Large Array (VLA) observation for 6.7 GHz and 12 GHz class II methanol, 22 GHz water masers, and continuum in the C, Ku, and K bands.
Results. The VLA overview revealed the presence of five distinct radio-continuum sources (CM1-4 and N) in G11.497-1.485. The central source, CM1, is found to show signs of accretion disc fragmentation, highlighted by the centimetre-continuum-traced fragments, and is found to drive a high-energy jet, the ends of which are marked by non-thermal knots CM2 and CM3. CM1 showed a gradual flaring of methanol masers and a fading of a 22 GHz water maser, which might be signalling an accretion burst. The two remaining sources of the region, CM4 and N, make up one of the most compact jet and disc–jet systems found to date.
Conclusions. The obtained data reveal, for the first time, the structure of the G11.497-1.485 region. The change in fluxes of the maser and the continuum emission confirm a transient event and reveal its impact on multiple sources in the region.
Spontane femorale arterio-venöse Fistel bei einer 80-jährigen Patientin als Zufallsbefund
Authors: Stammler Frank 
Published: January 2024

Zusammenfassung
Einführung Spontane arteriovenöse Fisteln (AVF) sind im Gegensatz zu iatrogenen oder posttraumatisch bedingten Fisteln extrem selten und in der Literatur nur vereinzelt publiziert. Die Diagnose einer AVF kann eine Herausforderung darstellen, insbesondere, wenn es sich um einen Zufallsbefund handelt.
Anamnese Eine 80-jährige Patientin stellte sich wegen Schwellneigung des linken Beines bei Varikosis in unserer phlebologischen Sprechstunde vor. Seit Jahren bemerke sie zudem, dass der rechte Fuß kühler sei. Katheter-Untersuchungen über die Leiste seien nicht durchgeführt worden, an Leistenverletzungen könne sie sich nicht erinnern.
Untersuchung und Befunde Eine Stammvarikosis der Vena saphena magna bestätigte sich klinisch und sonografisch. Zudem wirkte bei erhaltenen peripheren Pulsen der rechte Fuß leicht kühler, jedoch nicht diskoloriert. Auskultatorisch imponierte an der rechten Leiste ein systolisch-diastolisches Maschinengeräusch, einhergehend mit palpablem Schwirren. Farbduplex-sonografisch bestand zwischen der A. femoralis communis (AFC) und der Vena saphena accessoria anterior (VSAA) ein grobes Farbmosaik im Sinne eines Aliasings („Konfetti-Phänomen“). Es konnte ein Fistelkanal zwischen der AFC und der VSAA mit hohen systolisch-diastolischen Strömungsgeschwindigkeiten dargestellt werden. Im Bereich der Crosse-nahen Vena femoralis communis lag eine pulsatile und turbulente Strömung vor.
Therapie und Verlauf Bei symptomatischer Stammvarikosis am linken Bein wurde eine endovenöse Laserablation durchgeführt. Unter ultraschallgesteuerter Kompression der afferenten Arterie und Fistel sistierte der Fistelflow nicht (negatives Auslöschphänomen). Die Patientin reagierte hinsichtlich eines vorgeschlagenen interventionellen Verschlusses der AVF zurückhaltend. In Verlaufskontrollen über mittlerweile 4 Jahre entwickelten sich keine Zeichen einer kardialen Insuffizienz oder kritischen Extremitätenischämie.
Diskussion Spontane femorale AVF stellen eine Rarität dar. Charakteristische klinische Befunde führen zu einem gezielten Einsatz der Farbduplex-Sonografie mit korrekter Interpretation der Artefakte, die ansonsten leicht übersehen werden können.
Kein Nice-to-have
Authors: Heeser Alexandra 
Published: January 2024

ZusammenfassungDas seit 2020 in Kraft getretene Pflegeberufegesetz hat erstmalig Vorbehaltsaufgaben für die Pflege gesetzlich verankert. Sie sind essenziell für die Selbstbestimmung sowie für die Eigenverantwortung des Berufes. Dennoch scheint es an der Umsetzung noch zu hapern.
Aspergillus and the Lung
Authors: Janssens Iris , Lambrecht Bart N. , Van Braeckel Eva 
Published: January 2024

AbstractThe filamentous fungus Aspergillus causes a wide spectrum of diseases in the human lung, with Aspergillus fumigatus being the most pathogenic and allergenic subspecies. The broad range of clinical syndromes that can develop from the presence of Aspergillus in the respiratory tract is determined by the interaction between host and pathogen. In this review, an oversight of the different clinical entities of pulmonary aspergillosis is given, categorized by their main pathophysiological mechanisms. The underlying immune processes are discussed, and the main clinical, radiological, biochemical, microbiological, and histopathological findings are summarized.
Pulmonary Aspergillosis in People with Cystic Fibrosis
Authors: Schwarz C. , Eschenhagen P.N. , Mainz J.G. , Schmidergall T. , Schuette H. , Romanowska E. 
Published: January 2024

AbstractIn the last decade, fungal respiratory diseases have been increasingly investigated for their impact on the clinical course of people with cystic fibrosis (CF), with a particular focus on infections caused by Aspergillus spp. The most common organisms from this genus detected from respiratory cultures are Aspergillus fumigatus and Aspergillus terreus, followed by Aspergillus flavus, Aspergillus niger, and Aspergillus nidulans. These species have been identified to be both chronic colonizers and sources of active infection and may negatively impact lung function in people with CF. This review article discusses definitions of aspergillosis, challenges in clinical practice, and current literature available for laboratory findings, clinical diagnosis, and treatment options for pulmonary diseases caused by Aspergillus spp. in people with CF.
Statistical Evaluation of 231 Ears That Received Tympanic Membrane Perforation Closure Using Carbon Dioxide Laser and Collagen Sponge
Authors: Kuroda Kazuhiro , Kuroda Tatsuaki 
Published: January 2024

AbstractThe tympanoplasty technique reported by Yuasa et al, wherein autologous tissue is fixed to the perforated tympanic membrane using fibrin glue, is widely used. This technique has also led to the development of several simpler surgical methods using artificial materials not involving the use of fibrin glue. We used a carbon dioxide laser device (OtoLAM, Lumenis Be Japan Inc.) during tympanoplasty to refresh the perforation margin of the tympanic membrane and a collagen sponge with a silicone membrane (Terudermis, Alcare Inc.) as the closure material for tympanoplasty. OtoLAM is used to refresh the perforation margin in a short time. By simply fixing Terudermis to the perforated area using the attached silicone membrane, the surgery can be performed in a short time without the need for autologous tissue collection. For the past 9 years and 5 months, from June 2013 to November 2022, we used this method for tympanic membrane perforation closure in 231 ears. The closure rate after a single operation was not high (78/231; 33.8%) but significantly improved with multiple attempts (161/231; 69.7%). The advantages of this technique are that it is simple, can be performed quickly, is economical as it does not involve the use of fibrin glue, and there is no possibility of blood infection, eliminating the need for a detailed explanation to the patient.
An empirical analysis of SDG disclosure (SDGD) and board gender diversity: insights from the banking sector in an emerging economy
Authors: Mazumder Mohammed Mehadi Masud 
Published: January 2024

Abstract
There has been a recent surge in interest in academic research and public discourse surrounding the Sustainable Development Goals (SDGs). This study examines the level of SDG disclosure (SDGD) in Bangladeshi banking companies and explores its relationship with board gender diversity. An automated keyword search is applied to measure SDGD in the annual reports of publicly traded commercial banks. Based on agency, resource dependence, and stakeholder theories, this study hypothesises that a higher presence of female directors on the board is associated with greater commitment and contribution to SDGs, as evident in SDGD. The findings reveal a consistent increase in SDGD from 2015 to 2020 and confirm a significant positive relationship between board gender diversity and SDGD. The results remain consistent following robustness tests, including one-step and two-step system (dynamic) GMM estimations. Also, the study provides evidence that a critical mass of at least three female directors is necessary to establish a significant positive relationship between board gender diversity and SDGD. The implications of this study are twofold: firstly, it provides preliminary insights into the state of SDGD in the banking industry, which can inform regulators in assessing corporate engagement to SDGs and issuing disclosure guidance for the interest of the stakeholders. Secondly, the study highlights the importance of gender diversity on boards, as more female representation is associated with increasing SDGD. Such empirical findings imply that banking companies should consider appointing more female directors to their boards to advance SDG initiatives. Policymakers can utilise these findings to formulate regulations, particularly regarding the minimum number of female directors on corporate boards. This research contributes to the limited SDGD literature in the emerging economy context, offering insights into how board gender diversity is associated with SDGD.
Development and validation of a cryogenic far-infrared diffraction grating spectrometer used to post-disperse the output from a Fourier transform spectrometer
Authors: Anderson Alicia M. , Naylor David A. , Gom Brad G. , Buchan Matthew A. , Christiansen Adam J. , Veenendaal Ian T. 
Published: January 2024

Recent advances in far-infrared detector technology have led to increases in raw sensitivity of more than an order of magnitude over previous state-of-the-art detectors. With such sensitivity, photon noise becomes the dominant noise component, even when using cryogenically cooled optics, unless a method of restricting the spectral bandpass is employed. The leading instrument concept features reflecting diffraction gratings, which post-disperse the light that has been modulated by a polarizing Fourier transform spectrometer (FTS) onto a detector array, thereby reducing the photon noise on each detector. This paper discusses the development of a cryogenic (4 K) diffraction grating spectrometer that operates over the wavelength range of 285 to 500 μm and was used to post-disperse the output from a room-temperature polarizing FTS. Measurements of the grating spectral response and diffraction efficiency are presented as a function of both wavelength and polarization to characterize the instrumental performance.