bluuebunny/crossref_metadata_2025_split huggingface dataset:
End-of-Life Care in ICU in Saudi Arabia: perceptions of Charge Nurses
Authors: MANI Zakaria 
Published: January 2024

Abstract
Aim: the aim of this study was to explore the intensive care unit charge nurses' perceptions of end-of-life care in Saudi Arabia.
Design: a qualitative content analysis
Method: semi-structured interviews were conducted. Senior intensive care unit charge nurses were interviewed in the ICU meeting room of their units. The manifest qualitative content analysis method was used to analyse the transcribed data and then refining them into meaning unit, condensation, interpretation, sub-theme and theme.
Result: four themes were emerged including lack of family’s knowledge about their dying patient's condition, the need for EoLC awareness, language barriers, and education and preparation. The finding also indicated the requirement for end-of-life care guidelines, education, and preparation.
Conclusion: the issues of providing end-of-life care in the intensive care unit in Saudi Arabia can influence nurses' ability to meet the patients and their families' needs. Clear end-of-life care guidelines, education and training for nurses including Saudi’s Islamic culture, and multi professionals’ teamwork are significant to meet patients needs.
Edema melting length and therapeutic failure during hospitalization as factors associated with long-term mortality after basic treatment of severe acute malnutrition: Lwiro cohort follow-up in the Eastern Democratic Republic of the Congo
Authors: Lembebu Jean Corneille , Gaylord Amani Ngaboyeka , Bisimwa Ghislain , Neven Anouk , Makali Samuel Lwamushi , Chimanuka Christine , Chiribagula Christian Zalinga , Bugugu Bony Bonane , Bigirinama Rosine , Donnen Philippe , Mwene-Batu Pacifique 
Published: January 2024

Abstract
Purpose
The aim of this study was to identify the factors associated with long-term mortality in subjects treated for SAM in childhood 11 to 30 years after nutritional rehabilitation.
Methodology
Referring to the data collected from December 2017 to November 2018 from the Lwiro cohort; we updated this database with additional data. Records of subjects admitted for SAM between 1988 and 2007 were extracted from the archives of the pediatric hospital in Lwiro, South Kivu, Democratic Republic of Congo (DRC). A multivariate Cox proportional hazards regression was used to identify factors associated with long-term mortality.
Results
A total of 816 subjects were found to be alive and 119 died. The mean age at admission to nutritional rehabilitation was 46 months. Around two third of the subjects had edema, and in 6,8% of these subjects, edema had subsided after thirty days in hospital. Almost one in ten (9.5%) cases of SAM resulted in treatment failure. The risk of death was significantly higher in subjects with a history of therapeutic failure (hospital stay ≥ 45 days) and in those whose edema had melted late (≥ 30 days) during their hospitalization, with respective risks of HR = 1.98 (1.07; 3.67) and 2.81 (1.12; 7.03) respectively.
Conclusion
Good follow-up after hospital discharge is imperative to ensure the success of SAM management in the medium and long term. However, this follow-up must be more intensive in patients who have failed treatment and whose edema has melted late during hospitalization.
Association between interleukin-4 C589T polymorphism and asthma in Iraq: Ameta-analysis
Authors: Hameed Raghdah Maytham , Abood Mustafa 
Published: January 2024

Abstract
Background
Asthma is a common disease with a complex risk architecture involving both genetic and environmental factors. Several studies have identified an association between interleukin (IL)-4 C-589T and asthma risk; however, the results remain inconclusive.
Aims
to identify the effect of IL-4 C589T polymorphisms on asthma susceptibility in Iraq.
Material and methods
PubMed, Web of Science, Scopus, and Google Scholar databases were searched for only case-control studies published on Iraqi asthmatic. The methodological quality assessment of the included studies was performed based on the Newcastle-Ottawa Quality Scale (NOS). Based on the heterogeneity, we conducted a meta-analysis using random-effect models. Pooled odds ratios (ORs) with a 95% confidence interval (CI) were calculated using the allele (C vs. T), homozygous (CC vs. TT), heterozygous (CT vs. TT), dominant (CC þ CT vs. TT), and recessive (CC vs. CT þ TT) genetic models to assess the strength of the relationship between IL-4 C589T polymorphism and asthma risk. In addition, the stability of our analysis was evaluated by heterogeneity, sensitivity, and study design, and publication bias analysis.
Result
We included 5 case-control studies with a total of 359 cases and 245 controls. All genetic models showed a higher risk of asthma, the overall analyses were a highly statistically significant. There was moderate to high heterogeneity among the genetic models. However, sensitivity analysis revealed highly significant differences in the pooled odds ratios of the rs2243250 polymorphism between asthmatic patients and control groups.
Conclusion
The meta-analysis identified a significant association between the IL-4 C-589T polymorphism and asthma. However, the substantial result variability indicates the need for well-designed case-control studies with a large population to more accurately estimate this association.
Colloidal atomic layer deposition on nanocrystals using ligand-modified precursors
Authors: Green Philippe , Lecina Ona Segura , Albertini Petru , Newton Mark , Kumar Krishna , Boulanger Coline , Leemans Jari , Thompson Paul , Loiudice Anna , Buonsanti Raffaella 
Published: January 2024

Abstract

Atomic layer deposition (ALD) is a method to grow thin metal oxide layers on a variety of materials for applications spanning from electronics to catalysis. Extending ALD to colloidally stable nanocrystals promises to combine the benefits of thin metal oxide coatings with the solution processability of nanocrystals. However, challenges persist in applying this method, which relate to finding precursors that promote growth of the metal oxide while preserving colloidal stability throughout the process. Herein, we introduce a colloidal ALD method to coat nanocrystals with amorphous metal oxide shells using metal and oxygen precursors that act as colloidally stabilizing ligands. Our scheme, involves metal-amide precursors modified with solubilizing groups and oleic acid as the oxygen source. The growth of the oxide is self-limiting and proceeds in a layer-by-layer fashion. Our protocol is generalizable and intrinsically scalable. Potential applications in display, light detection and catalysis are envisioned.
Hospital referrals, exclusions from hospital care, and deaths among long-term care residents in the Community of Madrid during the March-April 2020 COVID-19 epidemic period: A multivariate time series analysis
Authors: Béland François , Zunzunegui Maria Victoria , Garcia-Lopez Fernando José , Pozo-Rodriguez Francisco 
Published: January 2024

Abstract

Background:
From March 6 to April 7, 2020 the Community of Madrid (CoM), Spain, issued interventions in response to the COVID-19 epidemic, including hospital referral triage protocols for long-term care facility (LTCF) residents (March 18–25). Those with moderate to severe physical disability and/or cognitive impairment were excluded from hospital referral. This research assesses changes in the association between daily hospital referrals and the deaths of LTCF residents attributable to the triage protocols.
Methods:
Daily hospital referrals and all-cause mortality from January to June 2020 among LTCF residents were obtained. Statistically significant changes in LTCF resident hospital referrals, and daily deaths in-hospital and in-LTCF were identified when testing for breaks and regimes in the time series. Multivariate time series analyses were conducted to test changes in the associations between LTCF resident hospital referrals with in-hospital and in-LTCF daily deaths as well as with daily deaths in the CoM population aged 65 + at the time triage protocols were implemented.
Results:
Among LTCF residents, hospital referrals declined sharply from March 6 to March 23, 2020. Increases in daily deaths at LTCFs occurred from March 7 to April 1 followed by a decrease reaching pre-epidemic levels after April 28. The daily ratio of in-hospital deaths to in-LTCF deaths reached its lowest values from March 8 to April 13, 2020. The four versions of the triage protocol, published from March 18 to March 25 had no impact on further changes in the association of hospital referrals with daily deaths of LTCF residents in-hospital or in-LTCF. Daily deaths in the CoM population aged 65 + were the main driver of daily deaths of LTCF residents.
Conclusions:
During March-April 2020, while hospital referrals decreased because of CoM governmental interventions, daily deaths of LTCF residents within LTCFs increased. Increases in hospital ICU bed occupancy and non-ICU bed occupancy by non-residents of LTCF suggest that the CoM government chose to protect hospitals from collapse by restricting hospital referrals from LTCFs without concurrently implementing crisis standards of care within LTCFs. CoM triage protocols sanctioned the already existing restrictions on hospital referrals of LTCF residents set by the CoM government.
Chrysosplenetin B induces apoptosis and inhibits metastasis of gastric cancer AGS cell by regulating reactive oxygen species-mediated signaling pathways
Authors: Xue Hui , Li Shu-Mei , Tang Yan-Jun , Cao Jing-Long , Hou Wen-Shuang , Wang An-Qi , Ren Wan-Xia , Jin Cheng-Hao 
Published: January 2024

Abstract

Chrysosplenetin B (CHR) is a flavonoid compound with various pharmacological activities. This study aimed to investigate the effect and mechanism of CHR on gastric cancer (GC). A cell counting Kit 8 assay results showed that CHR had a good cytotoxic effect in twelve types of GC cell lines. Annexin-V/PI staining, flow cytometry, and western blot analysis results showed that CHR induced mitochondrial-dependent apoptosis of AGS cells by decreasing mitochondrial membrane potential and increasing the expression levels of Bad/Bcl-2 homologous dimer proteins. Network pharmacological analysis results showed that there were twenty high-value signaling pathways correlated with CHR and GC, among which AKT, MAPK, and STAT3 signaling pathways were closely related to the CHR induced apoptosis signaling pathways on AGS cells. Further through western blot analysis results showed that the protein expression levels of p-AKT, p-ERK, and p-STAT3 were significantly decreased, while the protein expression levels of p-JNK and p-p38 were significantly increased. Moreover, reactive oxygen species (ROS) analysis results showed that CHR induced ROS accumulation on AGS cells as an initial signal to regulate downstream signaling pathways. Cell cycle results showed that CHR arrested the AGS cell cycle in the G2/M phase by regulating the ROS/AKT signaling pathway. Transwell and wound healing assay results showed that CHR inhibited the invasion and migration of AGS cells by regulating ROS/Wnt-3a/GSK-3β/β-catenin signaling pathway. In conclusion, CHR inhibited cell proliferation, induced cell apoptosis, arrested the cell cycle in the G2/M phase, and inhibited invasion and migration on AGS cells.
Molecular characterization of a Single negative stranded RNA virus from the rice blast fungus Magnaporthe oryzae isolate NJ39
Authors: Ding Hang , Wang Yao , Cong Li , Shuai Simin , An Hongliu , 方 守国 , Zhang Songbai , Deng Qingchao 
Published: January 2024

Abstract

A novel negative-sense single-stranded RNA mycovirus, designated as " Magnaporthe oryzae
mymonavirus 1" (MoMNV1), was identifed in the rice blast fungus Magnaporthe oryzae isolate NJ39. MoMNV1 has a single genomic RNA segment consisting of 10,515 nucleotides, which contains six open reading frames. The largest open reading frame contains 5837 bases and encodes RNA replicase. The six open reading frames have no overlap and are linearly arranged on the genome, but the spacing of the genes is small, with a maximum of 315 bases and a minimum of 80 bases. Genome comparison and phylogenetic analysis indicated that MoMNV1 is a new member of the genus Penicillimonavirusin the family Mymonaviridae.
Study on viscoelasticity and damping properties of OSA/PAAM hydrogel
Authors: Zhang Xiaoran , Zhang Xin , Wang Puying , Wang Xinze , Zhong Linlin , Ma Songmei , Xu Wenlong 
Published: January 2024

Abstract
The problems of vibration and noise have seriously affected people's production and life. Hydrogel can be used as damping material because of its good viscoelasticity, but the current damping material is difficult to meet the needs of actual production and life because of the lack of wide damping temperature and frequency range and high loss factor. In this work, ammonium persulfate was used as both initiator and oxidant to form a mixed double network hydrogel of polyacrylamide and oxidized sodium alginate. The double network hydrogel prepared by this method has good adhesion and mechanical properties. In addition, due to the adjustable viscoelasticity of the hydrogel, the hydrogel shows excellent damping properties. When the concentration of MBA is 0.02%, it can reach the temperature range of 0-125 ℃, and the damping factor is more than 0.3. The visualization experiment proves the practical application effect of the hydrogel, so it is expected to be used as a damping material in damping, noise reduction and other fields in the future.
Integrated analysis of single-cell and bulk RNA-sequencing reveals a novel signature based on NK cell marker genes to predict prognosis and immunotherapy response in gastric cancer
Authors: Sun Jian-Rong , Kong Chen-Fan , Ye Yi-Xiang , Wang Qin , Qu Xiang-Ke , Jia Li-Qun 
Published: January 2024

Abstract
Natural killer (NK) cells play essential roles in the tumor development, diagnosis, and prognosis of tumors. In this study, we aimed to establish a reliable signature based on marker genes in NK cells, thus providing a new perspective for assessing immunotherapy and the prognosis of patients with gastric cancer (GC). We analyzed a total of 1187 samples retrieved from the public database. We performed a comprehensive analysis of single-cell RNA-sequencing (scRNA-seq) data of gastric cancer and identified 377 marker genes for NK cells. By performing Cox regression analysis, we established a 12-gene NK cell-associated signature (NKCAS) for the Cancer Genome Atlas (TCGA) cohort, that assigned GC patients into a low-risk group (LRG) or a high-risk group (HRG). In the TCGA cohort, the areas under curve (AUC) value were 0.73, 0.81, and 0.80 at 1, 3, and 5 years. External validation of the predictive ability for the signature was then validated in the Gene Expression Omnibus (GEO) cohorts (GSE84437). The expression levels of signature genes were measured and validated in GC cell lines by real-time PCR. Moreover, NKCAS was identified as an independent prognostic factor by multivariate analysis. We combined this with a variety of clinicopathological characteristics (age, M stage, and tumor grade) to construct a nomogram to predict the survival outcomes of patients. Moreover, the LRG showed higher immune cell infiltration, especially CD8 + T cells and NK cells. The risk score was negatively associated with inflammatory activities. Importantly, analysis of the independent immunotherapy cohort showed that the LRG had a better prognosis and immunotherapy response when compared with the HRG. The identification of NK cell marker genes in this study suggests potential therapeutic targets. Additionally, the developed predictive signatures and nomograms may aid in the clinical management of GC.
Effect of hydrogen embrittlement on the adhesion strength and wear resistance of DLC-coated 316L stainless steel for application in hydrogen valves of FCEVs
Authors: Kim Seong-Jong , Shin Dong-Ho 
Published: January 2024

Abstract

Diamond-like carbon (DLC) coating is a surface coating technology with excellent hydrogen permeation resistance and wear resistance. However, it is difficult to completely prevent hydrogen permeation, and when hydrogen penetrates into the coating layer, the DLC coating is adversely affected. Therefore, we investigated the effect of hydrogen embrittlment on the adhesion strength and wear resistance of the DLC coating layer. As the results of the research, the surface roughness of the DLC coating was increased by a maximum of 3.8 times with hydrogen charging, and the delamination ratio of the DLC coating reached about 58%. In addition, the Lc3, which refers to the adhesion strength corresponding to the complete delamination of the DLC coating, was decreased by a maximum of 2.0 N due to hydrogen permeation. In addition, the wear resistance decreased due to hydrogen permeation, and the exposed width of the substrate due to wear increased by more than 4 times. It was also determined that hydrogen blistering or hydrogen-induced cracking occurred at the interface between the DLC coating and the chromium buffer layer due to hydrogen permeation, which decreased the durability of the DLC coating.
Cluster analysis of Noncommunicable disease risk factors among adults in Brunei: The National Health and Nutritional Status Survey based on WHO’s STEPwise methodology
Authors: Rahman Hanif Abdul , Ong Sok King , Arifin Evi Nurvidya , Ahmad Siti Rohaiza , Murang Zaidah Rizidah , Tuah Nik AA , Razak Lubna Abdul , Lai Daphne Teck Ching , Chong Chean Lin , Naing Lin 
Published: January 2024

Abstract

Background: Noncommunicable diseases (NCDs) constitute a major global health concern, accounting for 74% of annual deaths worldwide. In Brunei Darussalam, the population faces high prevalence of risk factors for NCDs. To monitor progress of interventions aimed at reducing NCDs, Brunei conducted the third National Health and Nutritional Survey (NHANSS) using WHO STEPs methodology in 2022-2023.
Methods: Data from the NHANSS, comprising of 2,832 adults aged 18 to 69 in Brunei Darussalam were extracted and analysed. Clustering analysis was applied to identify naturally occurring clusters in the population based on NCD risk factors.
Results: The analysis revealed five distinct clusters. Cluster 1 exhibited highest prevalence (79%) of insufficient fruit and vegetable intake. Clusters 2 and 4, predominantly male, exhibited smoking, low fruit and vegetable intake, and abdominal obesity despite physical activity. Cluster 3, mainly comprised of women and older adults, demonstrated highest physical inactivity. Cluster 5, comprised of non-smokers with lower incidences of diabetes mellitus and cardiovascular diseases among high income group.
Conclusions: Effective population-based strategies, in line with the World Health Organization's recommendations, were proposed based on each cluster's characteristics. This underscores the Brunei government's commitment to implementing preventive measures and tailored interventions to mitigate the harmful impact of noncommunicable diseases.
Morpho-agronomic traits in Phaseolus lunatus L.: systematic review and meta-analysis as a tool in genotype selection
Authors: Dantas Saulo de Jesus , Torres Maria Fernanda Oliveira , Álvares-Carvalho Sheila Valéria , Leal Gildemberg Amorim , Silva-Mann Renata , Vargas Pablo Forlan 
Published: January 2024

Abstract

This study estimated the mean values of morpho-agronomic traits of Phaseolus lunatus L. through a systematic review and meta-analysis. Seven studies were selected through the systematic review, which encompassed a total of 104 genotypes. The selected traits were pod length and width, number of seeds per pod, grain yield, and 100-seed weight. In the exploratory analysis, normality, descriptive parameters, box-plot graph, and quantile-quantile plot of the data were evaluated. In the meta-analysis, the Q test and I² statistic were applied, and then, the combined effect model and fixed effect model were tested, using the Genes and R software. The data showed a normal distribution, and the box-plot graph indicated the need to remove some outliers. The quantile-quantile plot showed no publication bias. Q values were high for most traits, indicating that the studies were not homogeneous, and the random effects model was adopted. The combined estimates of pod length, width, number of seeds, grain yield, and 100-seed weight were 69.80mm, 16.29mm, 2.69, 395.97kg/ha, and 48.61g, respectively. The values obtained were precise, due to their similarity with the mean values, indicating that the methodology is adequate for generating mean values of agronomic interest.
COVID-19 and the risk of acute cardiovascular diseases: A two-sample Mendelian randomization study
Authors: Li Yuling , Yang Dongliang , Kang Jian , Cao Yaming , Cui Liwang , Liu Funan 
Published: January 2024

Abstract
Background
Evidence suggests that coronavirus disease 2019 (COVID-19) is associated with the risk of cardiovascular diseases (CVDs). However, the results are inconsistent, and the causality remains to be established. We aimed to investigate the potential causal relationship between COVID-19 and CVDs by using two-sample Mendelian randomization (MR) analysis.
Methods
Summary-level data for COVID-19 and CVDs including myocarditis, heart failure (HF), acute myocardial infarction (AMI), arrhythmia and venous thromboembolism (VTE) were obtained from the IEU OpenGWAS project, a public genome-wide association study (GWAS). Single nucleotide polymorphisms (SNPs) were used as instrumental variables. Five complementary MR methods were performed, including inverse variance weighted (IVW), MR-Egger, weighted median, weighted mode and simple mode methods. IVW method was considered as the primary approach. Besides, sensitivity analyses, including Cochran's Q test, MR-Egger intercept test, and leave-one-out analysis, were performed to evaluate the robustness of the results.
Results
According to the IVW results, our MR study indicated that genetically predicted COVID-19 was not causally connected with the risk of CVDs [myocarditis: odds ratio (OR) = 1.407, 95% confidence interval (CI) = 0.761–2.602, p-value = 0.277; HF: OR = 1.180, 95% CI = 0.980–1.420, p-value = 0.080; AMI: OR = 1.002, 95% CI = 0.998–1.005, p-value = 0.241; arrhythmia: OR = 0.865, 95% CI = 0.717–1.044, p-value = 0.132; VTE: OR = 1.013, 95% CI = 0.997–1.028, p-value = 0.115]. The supplementary MR methods showed similar results. Sensitivity analyses suggested that the causal estimates were robust.
Conclusion
This two-sample MR analysis did not provide sufficient evidence for a causal relationship between COVID-19 and the risk of acute CVDs, which may provide new insights into the prevention of acute CVDs in COVID-19 patients.