bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of formononetin as the active compound of CR‐SR in hepatocellular carcinoma treatment: An integrated approach combining network pharmacology and weighted gene co‐expression networks
Authors: Li Chun , Xie Yuxin , Hu Shaoyu , Yu Hong , Xu Yunke , Shen Hongping , Yuan Yuan , Gu Long , Pu Bangming 
Published: October 2023

AbstractHepatocellular carcinoma (HCC) is a life‐threatening disease for which there is no cure. Traditional Chinese medicine is a treasure trove of Medicinals that has been used for thousands of years. In China, the traditional herb pair, Curcumae Rhizoma and Sparganii Rhizoma (CR‐SR) represent a classic herbal combination used for the treatment of HCC. However, the drug targets and pharmacological mechanism of action of CR‐SR in the treatment of HCC are unclear. To address this, we screened the active components and drug targets of CR‐SR from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and a high‐throughput experiment‐ and reference‐guided database of traditional Chinese medicines (HERB database). Combined with the weighted co‐expression network analysis of dataset GSE76427, we constructed an active component‐target‐disease regulatory network. It was found that CR‐SR's active components for HCC treatment included trans‐gondoic acid, beta‐sitosterol, stigmasterol, hederagenin, and formononetin. These compounds specifically targeted the genes Estrogen Receptor 1 (ESR1), Cyclin A2 (CCNA2), Checkpoint Kinase 1 (CHEK1), and Nuclear Receptor Coactivator 2 (NCOA2). ESR1, CCNA2, and CHEK1 genes showed significant differences in survival prognosis, expression levels, and statistical significance during the pathological stage. Moreover, their high affinity for formononetin was determined through molecular docking analysis. Cell assays and high‐throughput sequencing were performed to reveal that the inhibitory effect of formononetin on HepG2 cell proliferation was related to hepatocyte metabolism and cell cycle regulation‐related pathways. This study provides insights into potential HCC treatments.
Occupational exposure to epoxy components and risk of dermatitis: A registry‐based follow‐up study of the wind turbine industry
Authors: Christiansen Alexandra Golabek , Kinnerup Martin Byskov , Carstensen Ole , Sommerlund Mette , Clausen Per Axel , Bønløkke Jakob Hjort , Schlünssen Vivi , Isaksson Marléne , Schmidt Sigrun Alba Johannesdottir , Kolstad Henrik Albert 
Published: October 2023

AbstractBackgroundAllergic contact allergy and dermatitis are frequently reported among epoxy‐exposed workers.ObjectivesTo determine the risk of dermatitis associated with epoxy exposure.MethodsWe followed 825 epoxy‐exposed and 1091 non‐exposed blue‐collar workers, and 493 white‐collar workers of a Danish wind turbine blade factory during 2017–2022 with linked data from national health registers on diagnoses, patch testing, or fillings of prescriptions for topical corticosteroids. Incidence rate ratios of dermatitis or a first‐time topical corticosteroid prescription were estimated with Poisson regression using non‐exposed blue‐collar workers as reference. We similarly estimated incidence rate ratios for the duration of epoxy exposure and current epoxy exposure.ResultsEpoxy‐exposed blue‐collar workers showed a dermatitis incidence rate of 2.1 per 100 000 person days, a two‐fold increased risk of dermatitis and a 20% increased risk of filling a prescription for topical corticosteroids. Incidence rate ratios were higher during early exposure and declined with further exposure for both outcomes. White‐collar workers had generally lower risks.ConclusionWe observed an increased risk of dermatitis following epoxy exposure confirming previous case reports and cross‐sectional studies emphasizing the need for intensified focus on preventive efforts for this group of workers.
An international survey exploring the management of pilonidal disease
Authors: Gallo Gaetano , Goglia Marta , Senapati Asha , Pata Francesco , Basso Luigi , Grossi Ugo ,   2022 European Society of Coloproctology (ESCP) collaborating group
Published: October 2023

AbstractAimPilonidal disease (PD) is a common debilitating condition frequently seen in surgical practice. Several available treatments carry different benefit/risk balances. The aim of this study was to snapshot the current management of PD across European countries.MethodMembers affiliated to the European Society of Coloproctology were invited to join the survey. An invitation was extended to others via social media. The predictive power of respondents' and hospitals' demographics on the change of therapeutic approach was explored.ResultsRespondents (n = 452) were mostly men (77%), aged 26–60 years, practising in both academic and public hospitals and with fair distribution between colorectal (51%) and general (48%) surgeons. A total of 331 (73%) respondents recommended surgery at first presentation of the disease. Up to 80% of them recommended antibiotic therapy and 95% did not use any classification of PD. A primary closure technique was the preferred procedure (29%), followed by open technique (22%), flap creation (7%), sinusectomy (7%) and marsupialization (7%). Approximately 27% of subjects would choose the same surgical technique even after a failure. Almost half (46%) perform surgery as office based. A conservative approach was negatively associated with acutely presenting PD (p < 0.001). Respondents who were not considering tailored surgery based on patient presentation tended to change their approach in the case of a failed procedure.ConclusionWith the caveat of a heterogeneous number of respondents across countries, the results of our snapshot survey may inform the development of future guidelines.
A novel method to assess copy number variations in melanocytic neoplasms: Droplet digital PCR for precise quantitation of MYC and MYB genes
Authors: Ramos‐Rodriguez Alvaro J. , McFadden Jason R. , Momtahen Shabnam , LeBlanc Robert E. , Yan Shaofeng , Chaudhari Advaita S. , Cloutier Jeffrey M. , Stevanovic Mirjana , Barney Rachael , Syku Marie , Lozano‐Franco Mario , Hughes Edward , Sriharan Aravindhan 
Published: October 2023

AbstractIntroductionWhile most melanocytic neoplasms can be classified as either benign or malignant by histopathology alone, ancillary molecular diagnostic tests can be necessary to establish the correct diagnosis in challenging cases. Currently, the detection of copy number variations (CNVs) by fluorescence in situ hybridization and chromosomal microarray (CMA) are the most popular methods, but remain expensive and inaccessible. We aim to develop a relatively inexpensive, fast, and accessible molecular assay to detect CNVs relevant to melanoma using droplet digital polymerase chain reaction (ddPCR) technology.MethodsIn this proof‐of‐concept study, we evaluated CNVs in MYC and MYB genes from 73 cases of benign nevi, borderline melanocytic lesions, and primary and metastatic melanoma at our institution from 2015 to 2022. A multiplexed ddPCR assay and CMA were performed on each sample, and the results were compared.ResultsConcordance analysis of ddPCR with CMA for quantification of MYC and MYB CNVs revealed a sensitivity and specificity of 89% and 86% for MYC and 83% and 74% for MYB, respectively.ConclusionWe demonstrate the first use of a multiplexed ddPCR assay to identify CNVs in melanocytic neoplasms. With further improvement and validation, ddPCR may represent a low‐cost and rapid tool to aid in the diagnosis of histopathologically ambiguous melanocytic tumors.
Review of the impact of the International System for Serous Fluid Cytopathology
Authors: Mikou Panagiota , Pergaris Alexandros , Engels Marianne , Chandra Ashish 
Published: October 2023

AbstractThe International System for Reporting Serous Fluid Cytology (TIS) has been proposed by an expert working team composed of the International Academy of Cytology and the American Society of Cytopathology, following an international survey. Since its introduction, the TIS has gained worldwide acceptance, and this review aims to assess its global impact. A literature search revealed 25 studies which have presented data on the impact of the TIS. Most of them provide data, including risk of malignancy (ROM) for each diagnostic category, separately for pleural, peritoneal and pericardial effusions, while a few do not separate them. A few studies focus on specific diagnoses like mesothelioma on specific types of fluids or more specific issues like the optimal fluid volume for cytology or interobserver variability. A synopsis of the data from the literature search is presented in four tables. The ROM assessment is discussed, as well as interobserver variability and the use of ancillary diagnostic immunochemistry. In conclusion, our review of the published data suggests that the TIS is a valid classification scheme that has been widely accepted by pathologists globally, is highly reproducible and makes a valuable contribution to clinical therapeutic management.
Abolishing β‐arrestin recruitment is necessary for the full metabolic benefits of G protein‐biased glucagon‐like peptide‐1 receptor agonists
Authors: Hinds Charlotte E. , Peace Ellie , Chen Shiqian , Davies Iona , El Eid Liliane , Tomas Alejandra , Tan Tricia , Minnion James , Jones Ben , Bloom Stephen R. 
Published: October 2023

AbstractAimEarlier studies have shown that peptide glucagon‐like peptide‐1 receptor (GLP‐1R) agonists with reduced β‐arrestin recruitment show enhanced anti‐hyperglycaemic efficacy through avoidance of GLP‐1R desensitization. However, the ligand modifications needed to decrease β‐arrestin recruitment usually also reduces GLP‐1R affinity, therefore higher doses are needed. Here we aimed to develop new, long‐acting, G protein‐biased GLP‐1R agonists with acute signalling potency comparable with semaglutide, to provide insights into specific experimental and therapeutic scenarios.Materials and MethodsNew GLP‐1R agonist peptides were assessed using a variety of in vitro and in vivo assays.ResultsFirst, we show that very substantial reductions in β‐arrestin recruitment efficacy are required to realize fully the benefits of GLP‐1R agonism on blood glucose lowering in mice, with more moderate reductions being less effective. Secondly, our lead compound (SRB107) performs substantially better than semaglutide for effects on blood glucose and weight loss, which may be jointly attributable to its biased agonist action and protracted pharmacokinetics. Thirdly, we show that biased agonist‐specific GLP‐1R internalization profiles occur at clinically relevant pharmacological concentrations. Finally, we show that SRB107 cAMP signalling is differentially modulated by single and double GLP1R coding variants seen in human populations, with implications for GLP‐1R agonist pharmacogenomics.ConclusionsCompletely abolishing β‐arrestin recruitment improves the anti‐hyperglycaemic effects of GLP‐1R agonists in mice.
Association between exposure to air pollution and blood lipids in the general population of Spain
Authors: Valdés Sergio , Doulatram‐Gamgaram Viyey , Maldonado‐Araque Cristina , García‐Escobar Eva , García‐Serrano Sara , Oualla‐Bachiri Wasima , García‐Vivanco Marta , Garrido Juan Luis , Gil Victoria , Martín‐Llorente Fernando , Calle‐Pascual Alfonso , Castaño Luis , Delgado Elías , Menéndez Edelmiro , Franch‐Nadal Josep , Gaztambide Sonia , Girbés Joan , Chaves F. Javier , Galán‐García José L. , Aguilera‐Venegas Gabriel , Vallvé Joan Carles , Amigó Núria , Guardiola Montse , Ribalta Josep , Rojo‐Martínez Gemma 
Published: October 2023

AbstractBackground and AimsWe aimed to assess the associations of exposure to air pollutants and standard and advanced lipoprotein measures, in a nationwide sample representative of the adult population of Spain.MethodsWe included 4647 adults (>18 years), participants in the national, cross‐sectional, population‐based di@bet.es study, conducted in 2008–2010. Standard lipid measurements were analysed on an Architect C8000 Analyzer (Abbott Laboratories SA). Lipoprotein analysis was made by an advanced 1H‐NMR lipoprotein test (Liposcale®). Participants were assigned air pollution concentrations for particulate matter <10 μm (PM10), <2.5 μm (PM2.5) and nitrogen dioxide (NO2), corresponding to the health examination year, obtained by modelling combined with measurements taken at air quality stations (CHIMERE chemistry‐transport model).ResultsIn multivariate linear regression models, each IQR increase in PM10, PM2.5 and NO2 was associated with 3.3%, 3.3% and 3% lower levels of HDL‐c and 1.3%, 1.4% and 1.1% lower HDL particle (HDL‐p) concentrations (p < .001 for all associations). In multivariate logistic regression, there was a significant association between PM10, PM2.5 and NO2 concentrations and the odds of presenting low HDL‐c (<40 mg/dL), low HDL‐p (<p25) and higher LDL particle (LDL‐p) concentrations (≥p75). In subgroup analyses there were stronger associations between PM10 and NO2 and low HDL‐p in men (p for interaction .008 and .034), and between NO2 and low HDL‐p in individuals with obesity (p for interaction .015).ConclusionsOur study shows an association between the exposure to air pollutants and blood lipids in the general population of Spain, suggesting a link to atherosclerosis.
Aggressiveness of Fusarium oxysporum and F. verticillioides isolates on stone and scots pine under greenhouse conditions
Authors: Olaizola Jaime , Pajares Juan A. , Gordon Thomas R. , Diez Julio J. 
Published: October 2023

AbstractScots pine (Pinus sylvestris) and Stone pine (Pinus pinea) are two important species used in re‐forestation that are subject to damage by damping‐off fungi in forest nurseries. Twenty‐two isolates of Fusarium oxysporum and F. verticillioides from diseased seedlings of eight different hosts were tested for aggressiveness on seeds and seedlings of both pine species, including the effects on seedling emergence and mortality. Scots pine was more susceptible to damping‐off than Stone pine, as indicated both by reduced seedling emergence and elevated seedling mortality. The impact of F. oxysporum and F. verticillioides on seeds and seedlings did not differ significantly for either pine species. Our findings support previous studies that found that these are damping‐off pathogens on the studied pines. Whereas most isolates proved to be pathogenic, some isolates of both Fusarium species showed to be non‐pathogenic.
Arbuscular mycorrhizal fungi benefit plants in response to major global change factors
Authors: Tang Bo , Man Jing , Lehmann Anika , Rillig Matthias C. 
Published: October 2023

AbstractLand plants play a key role in global carbon cycling, but the potential role of arbuscular mycorrhizal fungi (AMF) in the responses of a wide range of plant species to global change factors (GCFs) remains limited. Based on 1100 paired observations from 181 plant species, we conducted a meta‐analysis to test the role of AMF in plant responses to four GCFs: drought, warming, nitrogen (N) addition and elevated CO2. We show that AMF significantly ameliorate the negative effects of drought on plant performance. The GCFs N addition and elevated CO2 significantly enhance the performance of AM plants but not of non‐inoculated plants. AM plants show better performance than their non‐inoculated counterparts under warming, although neither of them showed a significant response to this GCF. These results suggest that AMF benefit plants in response to GCFs. Our study highlights the importance of AMF in enhancing plant performance under ongoing global change.
Migratory swans individually adjust their autumn migration and winter range to a warming climate
Authors: Linssen Hans , van Loon E. Emiel , Shamoun‐Baranes Judy Z. , Nuijten Rascha J. M. , Nolet Bart A. 
Published: October 2023

AbstractIn response to climate warming, migratory animals can alter their migration so that different events in the annual cycle are better aligned in space and time with suitable environmental conditions. Although such responses have been studied extensively during spring migration and the breeding season, much less is known about the influence of temperature on movements throughout autumn migration and how those movements result in a winter range and shifts therein. We use multi‐year GPS tracking data to quantify how daily autumn movement and annual winter distance from the breeding grounds are related to temperature in the Western Palearctic Bewick's swan, a long‐lived migratory waterbird whose winter range has shifted more than 350 km closer to the breeding grounds since 1970 due to individuals increasingly ‘short‐stopping’ their autumn migration. We show that the migratory movement of swans is driven by lower temperatures throughout the autumn season, with individuals during late autumn moving only substantially when temperatures drop below freezing. As a result, there is large flexibility in their annual winter distance as a response to winter temperature. On average, individuals overwinter 118 km closer to the breeding grounds per 1°C increase in mean December–January temperature. Given the observed temperature increase in the Bewick's swan winter range during the last decades, our results imply that the observed range shift is for a substantial part driven by individual responses to a warming climate. We thus present an example of individual flexibility towards climatic conditions driving the range shift of a migratory species. Our study adds to the understanding of the processes that shape autumn migration decisions, winter ranges and shifts therein, which is crucial to be able to predict how climate change may impact these processes in the future.
Asymmetric response of aboveground and belowground temporal stability to nitrogen and phosphorus addition in a Tibetan alpine grassland
Authors: Wang Yonghui , Wang Chao , Ren Fei , Jing Xin , Ma Wenhong , He Jin‐Sheng , Jiang Lin 
Published: October 2023

AbstractAnthropogenic eutrophication is known to impair the stability of aboveground net primary productivity (ANPP), but its effects on the stability of belowground (BNPP) and total (TNPP) net primary productivity remain poorly understood. Based on a nitrogen and phosphorus addition experiment in a Tibetan alpine grassland, we show that nitrogen addition had little impact on the temporal stability of ANPP, BNPP, and TNPP, whereas phosphorus addition reduced the temporal stability of BNPP and TNPP, but not ANPP. Significant interactive effects of nitrogen and phosphorus addition were observed on the stability of ANPP because of the opposite phosphorus effects under ambient and enriched nitrogen conditions. We found that the stability of TNPP was primarily driven by that of BNPP rather than that of ANPP. The responses of BNPP stability cannot be predicted by those of ANPP stability, as the variations in responses of ANPP and BNPP to enriched nutrient, with ANPP increased while BNPP remained unaffected, resulted in asymmetric responses in their stability. The dynamics of grasses, the most abundant plant functional group, instead of community species diversity, largely contributed to the ANPP stability. Under the enriched nutrient condition, the synchronization of grasses reduced the grass stability, while the latter had a significant but weak negative impact on the BNPP stability. These findings challenge the prevalent view that species diversity regulates the responses of ecosystem stability to nutrient enrichment. Our findings also suggest that the ecological consequences of nutrient enrichment on ecosystem stability cannot be accurately predicted from the responses of aboveground components and highlight the need for a better understanding of the belowground ecosystem dynamics.
Asymmetric impacts of surface thaw onset change on seasonal vegetation growth in Arctic permafrost
Authors: Chen Xing , Jeong Sujong 
Published: October 2023

AbstractAimChanges in surface thaw onset in Arctic permafrost can regulate terrestrial ecosystem dynamics. Permafrost surface thaw onset has advanced significantly because of strong Arctic warming. However, surface thaw onset change effects on vegetation during the different growing season stages remain unclear.LocationArctic and subarctic permafrost region.Time Period1982–2016.Major Taxa StudiedArctic vegetation.MethodsThis study investigated surface thaw onset change effects on vegetation growth in the Arctic permafrost region by analysing the relationships between surface thaw onset dates and vegetation indices obtained via satellite remote sensing from 1982 to 2016.ResultsThe leaf area index (LAI) from April to June was negatively correlated (−0.16 area‐averaged partial correlation coefficient) with surface‐thawed dates in over 78.6% of the Arctic permafrost region, and the LAI from August to October was positively correlated (0.17 area‐averaged partial correlation coefficient) with surface‐thawed dates in over 79.9% of the Arctic permafrost region. These distinct relationships suggest that earlier thaw onset may have a positive effect on vegetation growth during the early stage of the growing season. However, earlier thaw onset can limit vegetation growth during the late stage of the growing season. The different relationships between surface thaw onset and LAI can be explained by moisture availability. This difference occurred because 67.3% of the Arctic permafrost region was more constrained by temperature during the early stage of the growing season, therefore, the earlier thaw onset alleviated this temperature constraint and benefited vegetation growth. During the late stage of the growing season, 47.7% of the Arctic permafrost region was more constrained by moisture.Main ConclusionsOur results suggest that surface thaw onset change effects on vegetation vary by period, and this difference requires careful consideration when predicting ecosystem changes in permafrost regions.
A Tapestry of Black Anthropology of Freedom: Insights from James H. Cone and Frantz Fanon
Authors: Aihiokhai SimonMary Asese 
Published: October 2023

ABSTRACTAbstract: What is freedom without the ability to wonder and imagine new ways of being in the world? This question is at the heart of the works and contributions of Frantz Fanon and James H. Cone in their responses to the colonialities operating in the Black world, and the appropriate response to such colonialities through the medium of strategic alliances and a theological imagination of what it means to be human that is oriented towards blackness itself. However, since blackness is a production of white gaze, it is intended to embody pathologies of dehumanisation. Fanon and Cone do not shy away from shedding light on these pathologies. However, rather than slipping into the realm of nihilism, a pneumatological turn is articulated that allows for blackness to be a medium of encountering the gift of authentic humanity that is in solidarity with God's epiphany of life in the world. Fanon's and Cone's centring of rebellion as the pathway for an embrace of an anthropology of freedom is retrieved as a way of understanding the link between hope and a rich reading of anthropology of freedom that blackness evokes.