bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purifikasi fikosianin dari Spirulina platensis hasil intervensi kemangi (Ocimum basilicum) pada konsentrasi amonium sulfat berbeda
Authors: Yuliani Yuliani , Agustini Tri Winarni , Dewi Eko Nurcahya , Afifah Diana Nur 
Published: January 2024

Spirulina platensis merupakan kelompok ganggang hijau biru berserabut yang mengandung senyawa fikobiliprotein. Fikosianin merupakan komplek pigmen-protein paling tinggi pada fikobiliprotein. Fikosianin memiliki nilai komersial yang paling dominan pada industri makanan, kosmetik maupun obat-obatan. Purifikasi fikosianin merupakan suatu proses peningkatan kemurnian dan salah satu cara untuk meningkatkan aktivitas spesifik fikosianin. Ocimum basilicum atau kemangi adalah tumbuhan yang mengandung azulene yang merupakan senyawa hidrokarbon aromatik yang berwarna biru. Tujuan penelitian ini adalah untuk menentukan pengaruh intervensi kemangi dan tingkat saturasi pengendapan amonium sulfat pada proses purifikasi fikosianin S. platensis terhadap indeks kemurnian, kandungan fikosianin, recovery, dan total protein. Metode purifikasi fikosianin yang digunakan adalah metode multistep yang terdiri dari ekstraksi S. platensis tanpa intervensi dan intervensi kemangi, pengendapan dengan amonium sulfat pada tingkat saturasi (0-20%), (20-50%), (50-70%), (70-90%), dan ultrafiltrasi (DF/UF). Hasil penelitian menunjukkan tingkat saturasi terbaik pada tahap purifikasi dengan pengendapan amonium sulfat untuk sampel tanpa intervensi sebesar 50% dan intervensi sebesar 70%. Fikosianin hasil intervensi kemangi memiliki nilai kemurnian dan kandungan fikosianin yang lebih tinggi dibandingkan sampel tanpa intervensi pada setiap tahapan purifikasi. Nilai kemurnian pada tahap purifikasi akhir sebesar 2,54 AU untuk sampel tanpa intervensi dan 2,57 AU untuk sampel intervensi kemangi. Perlakuan intervensi maupun tanpa intervensi daun kemangi pada S. platensis dapat menurunkan nilai recovery dan total protein pada setiap tahap purifikasi.
Adverse Childhood Experiences and Cardiovascular Disease Risks: Implications for North Carolina and the Need for an Upstream Approach
Authors: Hege Adam 
Published: January 2024

Adverse childhood experiences (ACEs) have a negative impact on people across the lifespan, including poorer health outcomes. Data have indicated that 60% of North Carolina adults have experienced at least one ACE, while concurrently heart disease is the leading cause of death. There is a need to study the linkages between the two, and researchers and leaders should take an upstream approach to doing so.
The Implementation of Merdeka Curriculum on Historical Subject at SMA Negeri 3 Jember
Authors: Setyawati Laily , Umamah Nurul , Sumardi Sumardi , Marjono Marjono 
Published: January 2024

The occurrence of learning loss during the Covid-19 pandemic resulted in decreasing students' knowledge & skill. In order to overcome the learning loss, the Ministry of Education and Culture issued a new breakthrough namely the merdeka Curriculum. The research's aim is to examine in depth (1) availability of facilities, infrastructure & human resources; (2) The implementation of Process and assessment standards of merdeka. The research method used is qualitative. Data collection techniques used are; observation, interview, and documentation. Data analysis consists of 3 stages: Data reduction, Data display and Conclusion & verification. The results of this research ; (1) The facilities are good & complete. The infrastructure has good condition, but it's not complete; (2) It has quality human resources;(3) The process standards are in accordance with planning, implementation, and assessment. The planning has not been changed while the implementation & assessment are different from the planning used. The assessment standards are carried out fairly, objectively and educatively. The assessment used is formative assessment.
Keywords: Independent curriculum, History Lesson, Facilities & Infrastructure, Process Standards, Assessment Standards
Implementation of Women's Movement Values in Java as History Learning Resources
Authors: Az-Zahra Aqilla 
Published: January 2024

The struggle of the women's movement in the Java region in obtaining rights and authorities which were initially opposed during the movement provided lessons for the community, especially students in history learning activities. The aim of the research is for students to take the value of the struggle from the women's movement as implementation in everyday life to advance education that has been built by women's movement figures during the period of the national movement. This research was conducted using a descriptive research type using a qualitative approach. Data collection techniques were carried out through literature studies by reviewing various sources ranging from articles, research-related literature, as well as analysis of movement discussions related to women. The value of the struggle of the Javanese women's movement can be implemented in history lessons by integrating Javanese women's movement material into the school curriculum and opening extracurriculars with the theme of the history of the Javanese women's movement. The struggle of the women's movement is expected to have a positive impact today such as gender empowerment, expansion of education, equal rights and authority or freedom and just leadership.
Keywords: Value of struggle, Javanese Women's Movement, Education, Rights and authority, Learning History.
Captain Wardiman's Way of Fighting the Dutch
Authors: Matanasi Petrik 
Published: January 2024

We traced the life of Captain Wardiman which has not been much discussed in Indonesian historical research. Captain Wardiman was one of the leading Javanese officers in the Dutch East Indies era. He is actually an interesting figure in the history of Indonesians in the Dutch East Indies colonial army. Captain Wardiman was considered brilliant in suppressing the East Kalimantan SI Pasir rebellion. He actually left Dutch military service when his income was high. He wanted to be a soldier again when Indonesia faced the Dutch army.
Keywords: Captain Wardiman’s, figthting, Dutch
The Israel-Palestine Sovereignty Struggle: A Historical Review Based On Territorial Claims
Authors: Pratama Affilah Putra , Wiratama Nara Setya , Budiono Heru 
Published: January 2024

The land of Palestine is holy land for the three major religions, namely Islam, Christianity and Judaism. Israel and Palestine are countries that claim that this land is their territory, so this has sparked a prolonged conflict between the two. This research aims to provide an overview of the history of territorial claims in the Israeli-Palestinian region, territorial claims based on an Israeli-Palestinian perspective, and efforts to resolve the Israeli-Palestinian conflict. Researchers use the historical method with a qualitative approach, which has stages of heuristics, criticism, interpretation and historiography. The research results concluded that the Israeli-Palestinian conflict is a complex problem. The involvement of religious, political and historical dimensions in it can influence a person's views and understanding of the Israeli-Palestinian territorial claims. Through this research, it can be seen that the importance of good diplomacy and mediation can encourage the creation of commitment and trust in each other, thereby creating a sense of tolerance which can be used as the key to fair sovereignty for both parties.
Keywords: Israel, Palestine, Islam, Judaism
Application of the Learning Contract Learning Method to History Learning Activities of Class X Students in Online Business and Marketing at State Vocational High School 1 Pontianak
Authors: Lidia Lidia , Ulfa Maria , Putri Astrini Eka 
Published: January 2024

This research was conducted with the aim of knowing the application of the learning contract learning method to the learning activities of class X BDP students at Vocational High School 1 Pontianak. This research is a quantitative research with experimental method. The data source was used by students of class X BDP 1 as the experimental class and class X BDP 2 as the control class. Data collection through questionnaires and documentation. From the results of the research the researchers found that: The implementation of learning history using the learning contract learning method includes planning by determining learning objectives, applying learning contract learning methods and evaluating. Student activities during the learning process showed that students were able to explain material about the origins of their ancestors and conduct group discussions. In the learning process using the learning contract learning method there is an influence on the history learning activities of class X BDP SMK Negeri 1 Pontianak. As for learning activities such as arriving on time, listening to subject matter and having group discussions related to historical material. In this case the learning contract learning method aims to achieve what is planned as well as possible.
Keywords: Learning Menthods, Learning Contract, Activity
Management of marandu grass pasture increases soil carbon and nitrogen stocks in forest grazing systems in the brazilian cerrado
Authors: Anésio Arnon Henrique Campos , Santos Márcia Vitória , Frazão Leidivan Almeida , Teixeira Rafael Bastos , Tuffi-Santos Leonardo David , Pegoraro Rodinei Facco , Barboza Josiane Aparecida de Souza , Dumont Mariana Almeida , Paschoaloto Josimari Regina , Silva Konrad Passos e 
Published: January 2024

Abstract
This paper aimed to evaluate the influence of management practices of marandu grass, at eucalyptus shading levels in a forest grazing system, on organic carbon (OC) and nitrogen (N) stocks, and on soil chemical characteristics in the Brazilian Cerrado. The experimental design adopted was entirely random with five repetitions. In the plots were allocated the environments with different light availability, and a treatment with marandu grass in full sun. The management of marandu grass pasture was distributed in the subplots with pre-pasture at 30, 40, 50, and 60 cm high. Evaluations were repeated in two crop cycles in year 1 and year 2. Soil chemical characteristics, OC and N stocks were analyzed. The management of marandu grass at 50 cm height in the forest grazing system provided higher levels of OC, N, organic matter in the soil and an increase in fertility, compared to monoculture pasture, with repercussions in two years. The integration of eucalyptus and marandu grass pasture contributes with greater stocks of OC and N in the soil strips closest to the tree row, due to greater nutrient cycling. Marandu grass in forest grazing systems with shading varying from 40 to 60% present greater efficiency and maintenance of soil chemical attributes, demonstrating greater sustainability compared to monoculture pastures.
Immunoinformatics approach to Rift Valley fever virus vaccine design in ruminants
Authors: Oladipo Elijah Kolawole , Taiwo Oluseyi Rotimi , Teniola Fashanu Omotoyosi , Temitope Adedokun Praise , Boluwatife Akanni Motunrayo , Oluwaseyi Oyewale Isaac , Oladimeji Bolanle Victor , Taiwo Jonathan Iyanuoluwa , Adejumo Isaac Oluseun 
Published: January 2024

Abstract
Rift Valley fever is a new arboviral illness with serious public health implications. The disease, which is brought on by the Rift Valley fever virus (RVFV), is linked to severe economic losses in animals as well as a number of human ailments. There are currently no authorized live attenuated vaccines for human use, and they have been linked to increased risks of abortion in ruminants.
In this study, the use of reverse vaccination was applied in creating a vaccine using a segment for use in ruminants by exploring proteins in the M-glycoprotein segment. Glycoprotein sequences were retrieved from cattle, sheep and goats from endemic countries across Africa and subjected to computational analysis for B and T cell epitope prediction. Highly conserved, immunodominant epitopes were obtained and connected with the ideal linkers for proper conformation. A Bos taurus-specific beta-defensin was also added to increase immunogenicity. The secondary as well as tertiary structures of the vaccine were then determined by SOPMA and alphafold2, respectively.
Analysis of the vaccine’s physicochemical properties all yielded favorable results regarding its potential effectiveness and safety. Furthermore, molecular docking analysis was carried out using Toll-like receptors 7 and 8 to show molecular binding interactions. While in-silico analyses have yielded satisfactory results, translational research may be required to validate the vaccines’ effectiveness in animals.
Preferences for genetic interventions for SCA and Huntington’s disease: results of a discrete choice experiment among patients.
Authors: Os Nienke J.H. Van , Oosterloo Mayke , Grutters Janneke P.C. , Essers Brigitte A.B. , de Warrenburg Bart P.C. van 
Published: January 2024

Abstract

Background Although genetic interventions are on the horizon for some polyglutamine expansion diseases, such as subtypes of spinocerebellar ataxia (SCA) and Huntington’s disease (HD), the patients’ preferences regarding these new therapies are unclear. This study aims to what extent different characteristics of genetic interventions affect the preferences of patients with SCA and HD with regard to these interventions.Methods Manifest and premanifest patients with SCA or HD were recruited online by platforms of patient associations. The respondents conducted a questionnaire that included a discrete choice experiment (DCE). The experimental design included 24 choice sets, but these were divided into three blocks of eight to reduce the number of tasks per respondent. Each choice set included two alternative treatments and consisted of four attributes (mode and frequency of administration, chance of a beneficial effect, risks, and follow-up), each with three or four different levels. The forced choice-elicitation format was used. Data were analyzed by using a multinominal logistic regression model.Results Responses of 216 participants were collected. The mode and frequency of administration of a genetic intervention, as well as the chance of a beneficial effect both influence the choice for a genetic intervention. Respondents less prefer repeated lumbar punctures compared to a single operation. As expected, a higher beneficial effect of treatment was preferred. Risks and follow-up did not influence the choice for a genetic intervention. Completing the DCE appeared difficult for some respondents, in particular for patients in a more severe disease stage of HD.Conclusions The results can be used for the design and implementation of future genetic interventional trials and care pathways for patients with rare movement disorders such as SCA and HD.
Eigenspace-based Fuzzy C-Means with Large Language Model BERT for Topic Detection
Authors: Murfi Hendri , Agung Yudhistira Jinawi , Nurrohmah Siti , Satria Yudi , Za’in Choiru , Rahayu Dwi 
Published: January 2024

Abstract

Topic detection is the process of automatically identifying topics within text data. The manual execution of this task becomes challenging for large-scale datasets due to its labor-intensive nature, and it needs machine learning for automated processing. A prevalent method in topic detection is clustering through Eigenspace-based Fuzzy C-Means (EFCM), utilizing a standard TFIDF as the text representation. However, TFIDF only pays attention to the frequency of words and does not consider the semantics of these words in the text. Bidirectional Encoder Representation from Transformer (BERT) is a pre-trained model which means that it has learnt the representations of the words and sentences as well as the underlying semantic relations that they are connected and has shown significant advantages over text representation in many Natural Language Processing (NLP) tasks. This paper extends the EFCM model using BERT instead of the standard TFIDF as text representation. Then, we use TFIDF on each cluster (c-TFIDF) to generate the most frequent words to represent the topics. Our simulations show that the BERT representation improves topic coherence scores of the EFCM model for topic detection. If we measure the topic coherence scores using TC-W2V, the BERT-based EFCM model scores better on the two datasets. Meanwhile, the model achieves better on all three datasets using the CTC measurement unit.
Behavior-based dependency networks between places shape urban economic resilience
Authors: Yabe Takahiro , Garcia-Bulle Bernardo , Frank Morgan , Pentland Alex , Moro Esteban 
Published: January 2024

Abstract

Urban economic resilience is intricately linked to how disruptions caused by pandemics, disasters, and technological shifts ripple through businesses and urban amenities.
Disruptions, such as closures of non-essential businesses during the COVID-19 pandemic, not only affect those places directly but also influence how people live and move, spreading the impact on other businesses and increasing the overall economic shock. However, it is unclear how much businesses depend on each other in these situations.
Leveraging large-scale human mobility data and millions of same-day visits in New York, Boston, Los Angeles, Seattle, and Dallas, we quantify dependencies between points-of-interest (POIs) encompassing businesses, stores, and amenities.
Compared to places' physical proximity, dependency networks computed from human mobility exhibit significantly higher rates of long-distance connections and biases towards specific pairs of POI categories.
We show that using behavior-based dependency relationships improves the predictability of business resilience during shocks, such as the COVID-19 pandemic, by around 40% compared to distance-based models.
Simulating hypothetical urban shocks reveals that neglecting behavior-based dependencies can lead to a substantial underestimation of the spatial cascades of disruptions on businesses and urban amenities.
Our findings underscore the importance of measuring the complex relationships woven through behavioral patterns in human mobility to foster urban economic resilience to shocks.
Genome-guided isolation of Fervidibacter sacchari, an aerobic, hyperthermophilic polysaccharide-degrading specialist
Authors: Hedlund Brian , Nou Nancy , Covington Jonathan , Lai Dengxun , Mayali Xavier , Seymour Cale , Johnston Juliet , Jiao Jian-Yu , Buessecker Steffen , Mosier Damon , Muok Alise , Torosian Nicole , Cook Allison , Briegel Ariane , Woyke Tanja , Eloe-Fadrosh Emiley , Shapiro Nicole , Bryan Scott , Sleezer Savannah , Dimapilis Joshua , Gonzalez Cristina , Gonzalez Lizett , Noriega Marlene , Hess Matthias , Carlson Ross , Liu Lan , Li Meng-Meng , Lian Zheng-Han , Zhu Siqi , Liu Fan , Sun Xian , Gao Beile , Mewalal Ritesh , Harmon-Smith Miranda , Blaby Ian , Cheng Jan-Fang , Weber Peter , Grigorean Gabriela , Li Wen-Jun , Dekas Anne , Dodsworth Jeremy , Pett-Ridge Jennifer , Palmer Marike 
Published: January 2024

Abstract

Few aerobic hyperthermophiles degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic polysaccharide-degrading hyperthermophile, Fervidibacter sacchari, which was originally ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides as sole carbon sources from 65-87.5 °C and expresses 191 carbohydrate-active enzymes (CAZymes) according to RNA-Seq and proteomics, including 30 with unusual glycoside hydrolase (GH)109, 177, or 179 domains. Many CAZymes were also expressed in a proteolytic enrichment culture, and fluorescence in situ hybridization and nanoscale secondary ion mass spectrometry confirmed rapid assimilation of 13C-starch in spring sediments. Purified GHs were optimally active at 80-100 °C on eight different polysaccharides. Finally, we reassign Fervidibacteria as a class within phylum Armatimonadota, along with 18 other species, and trace the evolution of aerobic and anaerobic polysaccharide catabolism within the phylum. This study establishes Fervidibacteria as unique hyperthermophilic polysaccharide-degrading specialists in terrestrial geothermal springs.
The Epstein Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and ER-phagy
Authors: Masucci Maria 
Published: January 2024

Abstract

The synthesis of membrane and secreted proteins is safeguarded by an Endoplasmic Reticulum-associated Ribosome Quality Control (ER-RQC) that promotes the disposal of defective translation products by the proteasome or via a lysosome-dependent pathway involving the degradation of portions of the ER by macroautophagy (ER-phagy). The UFMylation of RPL26 on ER-stalled ribosomes is essential for activating the ER-RQC and ER-phagy. Here, we report that the viral deubiquitinase (vDUB) encoded in the N-terminal domain of the EBV large tegument protein BPLF1 hinders the UFMylation of RPL26 on ribosomes that stall at the ER, promotes the stabilization of ER-RQC substrates, and inhibits ER-phagy. We found that the vDUB does not have UFM1 deconjugase activity and does not prevent the UFMylation of the ER membrane protein CYB5R3. However, it copurifies with ribosomes in sucrose gradients and abrogates a ZNF598- and LTN1-independent ubiquitination event that appears to be required for RPL26 UFMylation. Physiological levels of BPLF1 impaired RPL26 UFMylation and promoted the accumulation of lipidated LC3-II in productively EBV-infected cells, pointing to an important role of the enzyme in regulating the translation quality control that allows the efficient synthesis of viral proteins and the production of infectious virus.
The arterial supply of pancreas: clinical and anatomical correlation
Authors: Kaur Simarpreet , Bathla Shilpa , Haokip Lamhoitheng , Sharma Abhishek , jain pooja , Yadav Anjoo 
Published: January 2024

Abstract

Purpose: The main arterial blood supply to pancreas is provided by splenic, superior (SPDA) and inferior pancreaticoduodenal arteries (IPDA). These arteries are susceptible to anatomical variations which affect the outcome in various embolization and invasive techniques especially involved in management of GI bleed. The present study was performed with the objective of streamlining variations of the arterial pattern, classify them and to document the presence of accessory arteries which will aid in success of various surgical and radiological interventions.Methods: The study was conducted on 23 cadavers during routine dissection. The origin, course, branching pattern of splenic as well as superior and inferior pancreaticoduodenal arteries were studied carefully.Results: significant variants were seen in case of SPDA & IPDA. The variants of SPDA and IPDA were classified based upon the origin, course, branching pattern and presence of accessory arteries into group I, II and III respectively.Conclusions-The knowledge of the rare anatomic variants can help the interventional radiologists to plan further treatment for aneurysms and prevent risk of hemorrhage during surgical procedures.