bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: Prepubertal-type testicular teratoma with local metastasis in a postpubertal patient
Authors: Ghirardelli Smith Olivia C. , Tsai Alexander K. , Zhong Minghao , Dejban Pegah , Nelson Andrew C. , Dolan Michelle , Antonarakis Emmanuel S. , Murugan Paari 
Published: February 2025

IntroductionWe report for the first time a case of a postpubertal patient presenting with a metastatic prepubertal-type testicular teratoma.Case discussionA 29-year-old male with a history of corrected unilateral cryptorchidism presented with progressive bilateral lower extremity edema. Imaging revealed an inferior vena cava thrombus associated with a complex mass. A left testicular ultrasound identified a solid lesion suggestive of a germ cell tumor, leading to a left radical orchiectomy, which revealed a mature pure teratoma with no evidence of germ cell neoplasia in situ (GCNIS). Excision of the retroperitoneal mass confirmed the presence of mature teratomatous elements without evidence of non-teratomatous germ cell tumor elements or cytological atypia. Fluorescence in situ hybridization (FISH) showed no evidence of gain of 12p, and next-generation sequencing showed no alterations in genes known to be associated with GCT.ConclusionThis case illustrates that pure mature teratomas lacking chromosome 12p abnormalities, GCNIS, and other dysgenetic features, occurring in postpubertal males, cannot invariably be classified into the benign prepubertal-type teratoma category. Contrary to current paradigm, in rare cases these may represent tumors with metastatic potential.
Impact of aerobic, resistance, and combined training on cardiometabolic health-related indicators in inactive middle-aged men with excess body weight and obesity
Authors: Mengistu Friew Amare , Lake Yehualaw Alemu , Andualem Molalign Enchalew , Miherete Yalemsew Demlie , Zewdie Solomon Adamu 
Published: February 2025

MethodsTwenty physically inactive men (49.15 ± 2.581 years) and BMI with 27.66 ± 0.91, participated in an 8-month training programme involving concurrent exercise (CT), resistance training (RT), and aerobic training (AT) program to determine the effects on fasting blood glucose (FBG), insulin resistance (IR), blood pressure (BP) and waist–to–hip ratio (WHR) in overweight and obese adult persons. This study was used a randomized repeated measures parallel experimental design.ResultsPre-to-post mean values of FBG, IR, SBP, DBP and WHR significantly decreased. Exercise modality had a significant effect on FBG (F (2, 26) = 10.656, p = 0.001, η2 = 0.571), with RT and CT showing greater reductions than AT. IR decreased more in RT than in AT (MD = 0.410 ± 0.101, p = 0.03). SBP also varied significantly between modalities (F (2, 26) = 13.103, p = 0.02, η2 = 0.528), with CT and RT showing larger reductions than AT. WHR differed significantly (F (2, 16) = 18.175, p = 0.001, η2 = 0.694), with AT and CT showing more reductions than RT. Diastolic blood pressure (DBP) showed no significant effect from exercise modality.ConclusionThese findings highlight the importance of tailored exercise interventions, with short rest RT and CT emerging as the most effective method for inactive overweight and obese individuals.
Dynamic molecular regulation of salt stress responses in maize (Zea mays L.) seedlings
Authors: Maimaiti Atikaimu , Gu Wei , Yu Diansi , Guan Yuan , Qu Jingtao , Qin Tao , Wang Hui , Ren Jiaojiao , Zheng Hongjian , Wu Penghao 
Published: February 2025

IntroductionMaize ranks among the most essential crops globally, yet its growth and yield are significantly hindered by salt stress, posing challenges to agricultural productivity. To utilize saline-alkali soils more effectively and enrich maize germplasm resources, identifying salt-tolerant genes in maize is essential.MethodsIn this study, we used a salt-tolerant maize inbred line, SPL02, and a salt-sensitive maize inbred line, Mo17. We treated both lines with 180 mmol/L sodium chloride (NaCl) for 0 days, 3 days, 6 days, and 9 days at the three-leaf growth stage (V3). Through comprehensive morphological, physiological, and transcriptomic analyses, we assessed salt stress effects and identified hub genes and pathways associated with salt tolerance.ResultsOur analysis identified 25,383 expressed genes, with substantial differences in gene expression patterns across the salt treatment stages. We found 8,971 differentially expressed genes (DEGs)—7,111 unique to SPL02 and 4,791 unique to Mo17—indicating dynamic gene expression changes under salt stress. In SPL02, the DEGs are primarily associated with the MAPK signaling pathway, phenylpropanoid biosynthesis, and hormone signaling under salt treatment conditions. In Mo17, salt stress responses are primarily mediated through the abscisic acid-activated signaling pathway and hormone response. Additionally, our weighted gene co-expression network analysis (WGCNA) pinpointed five hub genes that likely play central roles in mediating salt tolerance. These genes are associated with functions including phosphate import ATP-binding protein, glycosyltransferase, and WRKY transcription factors.DiscussionThis study offers valuable insights into the complex regulatory networks governing the maize response to salt stress and identifies five hub genes and pathways for further investigation. These findings contribute valuable knowledge for enhancing agricultural resilience and sustainability in saline-affected environments.
A cluster randomized controlled trial examining the effects of a four-week mindfulness-based practice on primary school students’ interpersonal mindfulness, emotional intelligence, and attentional focus
Authors: Lin Li-Jen , Lin Yu-Hsun , Yu Su-Ping , Liu Thu-Hua , Chen Yi-Lang 
Published: February 2025

BackgroundAlthough mindfulness benefits are well-established across diverse populations, its impact on primary school children’s interpersonal mindfulness, emotional intelligence, and attentional focus remains underexplored. This study evaluated the effectiveness of a four-week mindfulness-based practice (MBP) intervention among primary school students.MethodsA cluster randomized controlled trial was conducted with 14 classes from Guanghua Elementary School, Taiwan. Classes were randomly assigned to either an intervention group (7 classes, n = 123) or a control group (7 classes, n = 158), encompassing middle and senior-grade male and female students. The intervention group participated in weekly 40-min MBP sessions over 4 weeks. Students completed three validated questionnaires measuring interpersonal mindfulness dimensions (Presence, PR; Awareness, AW; Nonreactivity, NR), emotional intelligence (EI), and mindfulness (MI, attentional focus), using 6-point Likert scales. Assessments were administered at baseline, immediately after the intervention, and at 8-week follow-up.ResultsBaseline scores revealed no significant differences between the groups across all measures. After the 4-week intervention, the intervention group showed significant improvements in PR (p &lt; 0.01), NR (p &lt; 0.01), and MI (p &lt; 0.001) compared to the control group. At the 8-week follow-up, improvements in PR and MI were maintained, while NR gains diminished. Three-way ANOVA identified that gender and grade significantly influenced AW (p &lt; 0.01) and PR (p &lt; 0.05), respectively, with stage effects observed for NR and EI (p &lt; 0.05). Notably, the intervention enhanced AW among boys, and gender-grade interactions had a significant impact on NR and EI outcomes.ConclusionOverall, the four-week MBP intervention significantly enhanced primary school students’ interpersonal mindfulness and attentional focus, with some benefits lasting up to 8 weeks. These findings highlight the importance of tailoring MBP programs to students’ developmental stages and gender-specific needs. Future studies should investigate longer interventions and incorporate objective measures to further validate these outcomes.
Nicotine replacement therapy as a smoking cessation tool for adolescents: an update
Authors: Beis Ioannis , Dimou Anastasios , Kotoulas Serafeim-Chrysovalantis , Pataka Athanasia 
Published: February 2025

BackgroundAdolescent smoking is a significant public health concern, as early nicotine addiction leads to more severe addiction and reduced cessation success during adulthood. While nicotine replacement therapy (NRT) is an effective smoking cessation tool in adults, its efficacy in adolescents is less clear.ObjectiveThis systematic review evaluates the effectiveness and safety of NRT for smoking cessation in adolescents.MethodsA comprehensive search of PubMed and Cochrane Library databases identified 12 studies (randomized controlled trials and observational) examining NRT in adolescents. Outcomes included smoking cessation rates, withdrawal symptom relief, smoking reduction, and adverse events.ResultsNRT demonstrated limited success in long-term smoking cessation among adolescents, with low cessation rates that often declined post-treatment. However, NRT was effective in reducing smoking frequency and in managing withdrawal symptoms in some cases. The safety profile was generally favourable, with mild side effects such as skin irritation, headaches, and nausea.ConclusionWhile NRT can reduce smoking and alleviate withdrawal symptoms, its effectiveness in sustaining long-term cessation in adolescents is limited. Adherence challenges and side effects suggest a need for complementary behavioural support and further research into tailored NRT strategies for this population.
Exercise addiction in college students: the impact of body dissatisfaction, stress, physical activity and gender
Authors: Wang Ying , Hua Guohuan , Liu Wenting , Wan Changsheng , Hao Ming , Zhang Mingshou 
Published: February 2025

IntroductionEngaging in physical activity is commonly regarded as beneficial to health. However, exercise addiction may arise when enthusiasm for exercise reaches a level that disrupts life balance and overall well-being. Factors influencing college students’ exercise addiction remain largely unknown.MethodsParticipants aged 18–23 years (N = 384) underwent body measurements, and sex-adapted silhouettes were employed to assess their level of body dissatisfaction. The Exercise Addiction Inventory was used to investigate the level of exercise addiction among college students. We used t-tests to compare sex differences in BMI, body fat percentage, body dissatisfaction levels, stress levels, and exercise addiction among college students. Chi-square’s test was used to compare differences between males and females in terms of BMI, physical activity levels, stress, and exercise addiction ratings. The exercise addiction level of college students was used as the dependent variable in multiple regression analysis, and BMI, muscle mass, body fat percentage, exercise score, stress level score, and body dissatisfaction level score were used as predictors.ResultsThe results of multivariate regression analysis revealed sex differences in physical activity scores, stress levels, body dissatisfaction, and exercise addiction levels, with males consistently exhibiting significantly higher scores than females. In males, body dissatisfaction scores and stress were significant predictors of exercise addiction. Among females, physical activity scores, stress, body dissatisfaction, and body fat percentage were identified as significant predictors of exercise addiction.DiscussionCollege students confront risks in relation to exercise addiction. High stress levels and body dissatisfaction may be important causes of exercise addiction. The male students had higher body dissatisfaction, stress levels, and risk of exercise addiction than the female students. With a change in female bodily aesthetics in terms of a shift from a primary focus on thinness-related beauty to having a thin and toned body, the risk of exercise addiction in female college students may increase.
Long-term consumption of green tea protects the mental health of middle-aged and older adult men by improving inflammation levels
Authors: Wan Zhenyu , Wan Qirong , Qin Xucong , Wang Gaohua , Lin Hong , Jin Yong , Wan Bing , Ai Linfeng , Wei Juan 
Published: February 2025

BackgroundMiddle-aged and older adult men are at a heightened risk of depression. Green tea, as a popular beverage, has drawn widespread attention for its health benefits. However, there remains controversy over the effects of green tea on combating depression and regulating hormones.ObjectiveThis study aimed to investigate the effects of long-term green tea consumption on depression levels, hormones, and brain structure in, middle-aged and older adult men.MethodsA total of 280 volunteers participated in the study, divided into a tea-drinking group and a control group. Basic demographic information and biological marker data, as well as MRI data from some of the volunteers, were collected. A controlled study was conducted to explore the effects of long-term tea drinking on them.ResultsBMI (p = 0.002), depression level (p = 0.003), insomnia severity (p = 0.008), and systemic inflammation index (p = 0.009) were significantly lower in the tea drinking group, and their testosterone levels were significantly higher than those in the control group (p = 0.001). Moreover, GM volume in the right precuneus in the control group was significantly reduced compared with that in the tea drinking group.ConclusionLong-term tea consumption helps reduce BMI and increase testosterone levels in middle-aged and older adult men, and it can also reduce their risk of depression by lowering inflammation and improving sleep quality. Additionally, long-term tea consumption may have the potential to delay brain aging in middle-aged and older adult men.
Climate of Accountability, Respect, and Ethics Survey (CARES): development and validation of an organizational climate survey
Authors: Martinson Brian C. , Smallfield Jarvis , Magley Vicki J. , Thrush Carol R. , Gunsalus C. K. 
Published: February 2025

BackgroundThis research describes the development and validation of the CARES Climate Survey, a 22-item measure designed to assess interpersonal dimensions of work-unit climates. Dimensions of work-unit climates are identified through work-unit member perceptions and include civility, interpersonal accountability, conflict resolution, and institutional harassment responsiveness.MethodsTwo samples (N = 1,384; N = 868) of academic researchers, including one from the North American membership of the American Geophysical Union (AGU), and one from a large research-intensive university, responded to the CARES and additional measures via an online survey.ResultsWe demonstrate content validity of the CARES measure and confirm structural validity through exploratory and confirmatory factor analyses which yielded four dimensions of interpersonal climate. In addition, we confirm the CARES internal reliability, construct validity, and excellent sub-group invariance.ConclusionsThe CARES is a brief, psychometrically sound instrument that can be used by researchers, institutional leaders, and other practitioners to assess interpersonal climates in organizational work-units.Originality/valueThis is the first study to develop and validate such a measure of interpersonal climates specifically in research-intensive organizations, using rigorous psychometric methods, grounded in both theory and prior research on work-unit climates.
Exploring the impacts of purslane extract: a novel supplement for improving physiological and immunological responses in Labeo catla fingerlings
Authors: Faisal Muhammad , Hussain Syed Makhdoom , Sarker Pallab K. , Ali Shafaqat , Al-Ghanim Khalid A. 
Published: February 2025

IntroductionThe application of therapeutic plants in aquaculture has gained considerable worldwide attention and is currently the focus of ongoing scientific research. These plants possess diverse bioactive compounds that offer safer, non-toxic, biodegradable, and biocompatible alternatives for consumers. This research assessed the efficacy of purslane extract supplementation on sunflower meal (SFM)-based diets (iso-nitrogenous, iso-lipidic, and iso-caloric) in terms of growth, improving carcass, mineral content, hematology, immune responses, and antioxidant status in Labeo catla fingerlings.MethodsEach of the seven test diets—T0 (no extract supplementation), T1 (0.5% supplementation), T2 (1% supplementation), T3 (1.5% supplementation), T4 (2% supplementation), T5 (2.5% supplementation), and T6 (3% supplementation)—were administered two times per day at a rate equivalent to 5% of their body mass. The experiment was maintained in V-shaped steel tanks for a duration of 90 days. Each group included 15 fingerlings (N = 315; average weight: 7.36 ± 0.03 g), and each diet was administered in triplicates.ResultsBased on the results of the current study, it is clear that the T3 (1.5% supplementation) diet showed significantly improved growth (in terms of percentage weight gain), feed utilization, and whole-body protein content. In addition, the incorporation of 1.5% purslane extract in SFM-based diets significantly enhanced hematological indices, including platelets, red blood cells, hemoglobin, and white blood cells. Furthermore, all the examined diets exhibited a significant (p &lt; 0.05) increase in mineral content. Dietary supplementation with 1.5% purslane extract exhibited significant enhancements in antioxidant defenses, including increased superoxide dismutase, catalase, and glutathione peroxide activities, alongside a marked decrease in malondialdehyde levels. Furthermore, immune parameters, such as lysozyme activity and total globulins levels, showed considerable improvements in response to the 1.5% purslane supplementation.DiscussionThis study suggested that 1.5% purslane extract supplementation is the optimum level for improving the health and physiology of L. catla fingerlings. The therapeutic benefits of purslane may be attributed to its bioactive compounds, which contribute significantly to enhancing fish performance.
IgE-Crosslinking-Induced Luciferase Expression Test as a Sensitive Indicator of Anisakis Allergy
Authors: Akiyama Haruyo , Niwa Masashi , Kurisaka Chisato , Hamada Yuto , Fukutomi Yuma , Teshima Reiko 
Published: February 2025

Background: Anisakis allergy has been increasing, and the diagnosis of it is based on specific serum IgE detection. Recently, the IgE-crosslinking-induced luciferase expression (EXiLE) test has been proposed as convenient tool for detecting functionally specific IgE antibodies. Here, we investigated if the EXiLE test is a useful tool in the diagnosis of Anisakis allergy. Methods: HuRa-40 cells were sensitized using six serum types from three patients with Anisakis allergy at the time of the initial test and after 6–12 months. Thereafter, various concentrations of Anisakis worm protein (AWP) were reacted to measure the degree of EXiLE. The degree of EXiLE was compared with Anisakis-specific IgE antibody levels measured by the CAP-FEIA method, and the IgE-antibody-binding protein profile was examined using IgE immunoblotting. Results: The results showed a good correlation between the CAP-FEIA values and EXiLE obtained with 5 μg/mL of AWP (R = 0.91, p < 0.01), a strong response on IgE immunoblotting in the region containing proteins weighing ≥40,000 Da. In addition, after the onset of Anisakis allergy, the degree of serum EXiLE decreased in two patients whose Anisakis-specific IgE antibody levels decreased over time but increased in one patient whose specific IgE antibodies increased after repeated antigen sensitization. Conclusions: Based on these data, the AWP-induced EXiLE test seemed to be useful and convenient for the diagnosis of Anisakis allergy, supplementing specific IgE determinants. After allergy onset, the use of this method to observe changes in specific IgE levels over time may be important for predicting the risk of recurrence.
Eficácia da Rotigotina no manejo dos sintomas da Doença de Willis-Ekbom: uma revisão de literatura
Authors: Ferrari João Pedro de Assis , Koga Anna Victoria Tetto , Moura Adriano Soler de , Silva Natalia Hirt Viana e , Oliveira Bárbara Alexandra Ossuchi de , Alves Giovanna de Lara Moreira , Macedo Luiz Felipe Amorim 
Published: February 2025

A síndrome das pernas inquietas ou doença de Willis-Ekbom (WED), do inglês Restless Legs Syndrome (RLS), é um distúrbio do ritmo circadiano sensório-motor que afeta profundamente a qualidade de vida do portador. Os agonistas dos receptores da dopamina, como a rotigotina, estão entre as terapias mais utilizadas para o tratamento da RLS. Desse modo, objetiva-se nesta revisão analisar a eficácia do uso da rotigotina na melhora do quadro da síndrome das pernas inquietas. Revisão integrativa com estudos em inglês publicados nas bases de dados MEDLINE–PubMed COCHRANE, EMBASE e Google Scholar nos últimos dez anos. Os termos de busca utilizados foram “rotigotine”, “restless legs syndrome”, “treatment” e o operador booleano “AND”. Apenas 04 estudos randomizados, duplo-cego, controlado por placebo foram selecionados, sendo incluídos um total de 649 participantes. De modo geral, a rotigotina apresentou efeitos benéficos no manejo da RLS, apresentando pouco efeitos adversos sistêmicos como náuseas e reações alérgicas no local da aplicação. A rotigotina foi benéfica na melhoria da gravidade geral dos sintomas de RLS a curto prazo na grande maioria dos estudos, indicando boa eficácia e risco relativamente baixo de piora do quadro. A perspectiva é que este estudo possa dar embasamento para futuras pesquisas clínicas que investiguem os mecanismos da rotigotina na síndrome das pernas inquietas de forma mais precisa, assim como seus efeitos a longo prazo, tornando-o parte da terapêutica segura.
Apneia do sono e acidente vascular cerebral: investigando a relação e impactos na saúde
Authors: Nakoula Eduarda , Chiaratti Bruno Ceschin , Roldan Mariana Malzoni , Neto Salim Mussi , Beraldi Rafaella Vercesi , Miranda Ana Beatriz Coral , Varaschin Nicolas Kubo , Mendes Gabriela Caramori , Vernay Bruna Almeida Faust Von Borell du , Oberderfer Aline Brizola 
Published: February 2025

A apneia obstrutiva do sono (AOS) é uma condição crônica caracterizada pela obstrução intermitente das vias aéreas durante o sono, resultando em hipoxemia e interrupções frequentes no ciclo de descanso. Evidências crescentes demonstram uma relação direta entre a AOS e o aumento do risco de acidente vascular cerebral (AVC), uma das principais causas de mortalidade e incapacidade de indivíduos globalmente. Compreender os mecanismos fisiopatológicos que conectam essas condições é essencial para a elaboração de intervenções preventivas e terapêuticas, visando à mitigação dos impactos da AOS na saúde neurológica.
Relationship between sound pressure distribution and drying effect for a wet cloth sample in an intense aerial standing wave sound field
Authors: Owada Chika , Asami Takuya , MIURA Hikaru 
Published: February 2025

Abstract
The authors have been studying the promotion of non-contact drying of wet cloth by forming a standing wave sound field in the air using intense aerial ultrasonic sources. In this paper, the sound pressure distribution with a wet cloth sample was examined, and the relationship between the sound field and the drying effect was investigated. When a high-water-content sample that was larger than the wavelength of the sound wave was placed at the sound pressure node, the standing wave sound field was changed by the reflection of the sound wave. However, the drying experiments showed that the drying effect was similar to that with the sample at the anti-node, demonstrating the usefulness of drying by ultrasonic waves.