bluuebunny/crossref_metadata_2025_split huggingface dataset:
A miniature of soluble methane monooxygenase enables
Escherichia coli to rapidly produce methanol with high yield
Authors: Lee Jeewon , Yu Yeonhwa , Shi Yongfan , Kwon Young-Wan , Choi Yoobin , Kim Yusik , Na Jeong-Geol , Huh June 
Published: October 2023

Abstract

Soluble methane monooxygenase (sMMO) oxidizes a wide range of carbon feedstocks (C1 to C8) directly using intracellular NADH and is a useful means in developing green routes for industrial manufacturing of chemicals; however, the high-throughput biosynthesis of active recombinant sMMO and the ensuing catalytic oxidation have so far been unsuccessful due to the structural and functional complexity of sMMO, comprised of functionally complementary, three enzyme components, which remains a major challenge for its industrial applications. Here we developed a catalytically active miniature of sMMO (mini-sMMO) through an optimal reassembly of minimal and modified components of sMMO on catalytically inert and stable apoferritin scaffold, with demonstrating the molecular characteristics in detail through in silico and experimental analyses and verifications. Notably, the in-situ methanol production in the high-cell-density culture of mini-sMMO-expressing recombinant Escherichia coli resulted in a remarkably higher productivity compared to the traditional methanotrophic production.
Enhancing student engagement in electronic platforms: e-gallery walk
Authors: Alkhateeb Nazdar Ezzaddin , Bigdeli Shoaleh , Mirhosseini Fakhrosadat 
Published: October 2023

Abstract
Background:
The COVID-19 pandemic was one of the most challenging situations that affected all aspects of humans’ life including education and active face to face teaching and learning methods.
Engaging learners in the teaching and learning process, teaching faculty members, and employing technologies to enhance teaching and learning requires adapting instructional methods based on diverse situations, especially in crises and force majeure.
Methods:
This study was an attempt to design and implement an electronic gallery-walk (e-gallery-walk) based on Gagne’s Nine Events of Instruction.
A teacher training e-course was designed, developed, and implemented during COVID-19 era at the center of pedagogy, training and academic development at Hawler Medical University, Iraq.
Moreover, for the purposes of this study different formative assessment tools were designed and implemented. Thirty-eight (n=38) MSc and PhD graduates from different medical specialties who were university instructors participated in this study and their learning was appraised via the designed assessment tools.
Results:
This hands-on-experience indicates that a carefully designed and implemented e-gallery walk enhances student-centered activity in an online faculty development program. This approach by improving student engagement results in more meaningful discussion and peer instruction.
The e-gallery walk gives teachers and students valuable experience of using technological advances and digital tools to modify face-to-face activities. These tools ensure successful teaching and learning practice in critical conditions when remote activities are deemed necessary.
Conclusion:
The study findings indicate that peer and teacher immediate feedback is highly influential on the quality of teaching and learning and a modified e-gallery walk by using Padlet could be a suitable choice to improve the process in virtual settings.
Prediction of birth weight using body measurements in Tunisian local goats raised in a low-input production system
Authors: AHLEM ATOUI , Carabaño M.J. , Mouna L. , Aicha Laaroussi , Mouldi mouldi , Samir Tlahig , Farah B.S. , Sghaier N. 
Published: October 2023

Abstract
The aim of this study is to establish linear measurements of local goat kids at birth and their factors of variation, as well as their possible correlations with birth weight. Additionally, the study aims to design statistical models and barymetric functions to predict birth weight of kids based on their morphometric data. The database includes data on 128 goat kids born to 89 goats and 9 bucks in the experimental goat herd at the El GORDHAB station of IRA. Average BW, withers height (L1), heart girth (L2), rump height (L3) and body length (L4) of all kids were 2.45kg, 32.66cm,30.56cm, 33.41cm and 31.21cm respectively. Results show that in general, local goat kids are small in size and weight at birth, which varies depending on sex, dam age and type of birth. Highest and positive correlation coefficient value between birth weight and heart girth were observed (r = 0.95). The coefficient of determination (R2) for heart girth (78%) was higher than other body measurements in single trait evaluation indicating it as the best trait for the predication of birth weight. The most appropriate combination of body measurements (R2 = 82%) was observed between height at withers and heart girth for predication of birth weight estimation. Developing a system for recording birth weight based on easily obtainable body measurements could be a useful approach for rural areas.
Discovery and epitypification of the sexual stage of Cadophora fallopiae on Fallopia spp. in Japan.
Authors: Itagaki Hiyori , Hosoya Tsuyoshi 
Published: October 2023

Abstract

The genus Cadophora was established as a dematiaceous asexual fungi characterized by solitary phialides with distinct collarettes, and phylogenetically suggested to be the asexual stage of the family Pyrenopezizaceae (Helotiales, Ascomycetes). However, the sexual stage is unknown except in a few species. Cadophora fallopiae which occurs on the overwintered stems of Fallopia in Europe, has only been described for its conidiomatous asexual stage. In Japan, C. fallopiae-like conidiomata were discovered associated with the apothecia of Pyrenopeziza sp. on the same substrate (the stem of Fallopia spp). Therefore, conspecificities of the apothecia and conidiomata were suspected. A detailed comparison of morphology and ITS-5.8S sequences confirmed that this fungus is identical to C. fallopiae known in Europe, and that the sexual stage was connected with its asexual stage for the first time. Based on the genetic diversity of single ascosporous isolates derived from a single apothecium, we hypothesized that the reason the sexual stage has not been discovered in Europe is the lack of a compatible mating type.
Lead and Cadmium have an impact on Oxidative Stress, Rumen and Blood Serum Constituents in Grazing Nubian Goats
Authors: Oraby Mohamed I , Baraka Taher A , Salem Noha Y , Rakha Gamal H 
Published: October 2023

Abstract
Environmental pollution, especially water pollution with heavy metals has a direct economic and health impact on the animal industry. This study investigated the impact of water pollution with heavy metals on grazing goats’ haemato-biochemical and oxidative stress parameters, as well as rumen function. Fifty Egyptian Nubian goats were divided into two groups: a control group (n = 13) raised indoors on clean tap water and an exposed group (n = 37) drinking from a polluted drainage water canal with cadmium (Cd) and Lead (Pb). The exposed goats showed significant reductions in RBCs, PCV, Hb, TLC, TP, albumin, globulin, Ca, and P, and significant elevations in MDA. Significant elevation in rumen pH in exposed goats compared to control goat was recorded. Significant reductions in TVFA, ammonia, AST, ALT, GGT, Ca and P were observed in exposed goats compared with the control goats. Positive correlation between Cd/Pb and rumen pH was recorded, while negative correlation was found in association with TVFA, Ammonia, AST, ALT, GGT, Ca and P. Pb and Cd levels were significantly elevated in the rumen and blood of exposed goats compared to control goats. The study concluded that goats watered in polluted areas suffered from alterations in hematological, serum biochemical, rumen fluid constituents, oxidative stress, and disturbance in mineral levels. These results highlight the harmful impact of heavy metals, particularly lead and cadmium, on goat’s health status and body function.
Epidemiological characteristics of neuroendocrine neoplasms in Beijing: a population-based retrospective study
Authors: Chi Yujia , Liu Shuo , Zhang Jianwei , Li Huichao , Yang Lei , Zhang Xi , Li Haoxin , Li Qingyu , Wang Ning , Lu Ming , Zhuo Minglei 
Published: October 2023

Abstract
Background
The incidence of neuroendocrine neoplasms (NENs) is rising rapidly worldwide. However, there are few reports on these heterogeneous diseases in China. Our study aimed to explore the epidemiological characteristics of NENs in Beijing.
Methods
We conducted a retrospective cohort study using population-based cancer surveillance data in Beijing, China. All data were extracted from the Beijing Cancer Registry with incidence dates from 1 January 1998 to 31 December 2018; the follow-up period was through 31 December 2021. Segi’s world standard population was used to estimate the age-standardized rate. Survival was estimated with the Kaplan-Meier method.
Results
From 1998 to 2018, the incidence of NENs in Beijing initially showed a significant increasing trend, from 1.07/100,000 to 4.03/100,000; this began to plateau after 2013. The age-specific incidence rate increased with age and peaked in the age group 70–74 years. The incidence in men was significantly higher than that in women (4.41/100,000 vs. 1.69/100,000). The most common sites of NENs were the lung (2.38/100,000) and rectum (0.14/100,000). Most NENs were diagnosed at a late stage. We found that NENs originating from the lung had worse overall survival than extrapulmonary NENs, and male patients had worse survival than female patients.
Conclusions
This study retrospectively analyzed the epidemiological characteristics of NENs in Beijing from 1998 to 2018. Our findings provide a reference regarding the epidemiological statistics of NENs in Beijing to contribute to the prevention, diagnosis, and treatment of these specific tumors.
A Champion, Any Champion: Leveraging Household Climate-Change or Energy-Efficiency Champions for Achieving Zero Emission Targets
Authors: Kleinschafer Jodie , Morrison Mark , Dowell David , Parton Kevin 
Published: October 2023

Abstract
Relationships between household members can be a significant determinant of the household’s overall stance towards energy efficiency and climate change. One understudied aspect of such within-household relationships is the role of champions. While the household champion is not a new concept, little previous research has examined the impact of champions on a household’s attitudes and actions directed towards energy efficiency and climate-change mitigation. Here, we use regression methods and a sample of 2,278 people drawn from the general population of Australia to show that household champions do significantly influence the way in which households regard these issues, and consequently the types of mitigation behaviour they engage in. There is a positive association between the presence of a champion and involvement, the perceived importance of the issue, efficacy, behaviour and behavioural intentions. While the presence of a climate-change champion was found to lead to a more substantive effect on behaviours, the presence of any champion in the household was found to have a meaningful influence on the extent of household energy-related behaviours. This was irrespective of whether they were championing energy efficiency or climate change, which household member they were and whether they were the respondent or not. These findings suggest the importance of differentiating strategies to drive behavioural change depending on whether there is someone to champion the cause in a household, as well as the type of champion present.
Upconverting-photon Quenching-mediated Perforation Influx as an Intracellular Delivery Method using posAuNP@UCNPs Nanocomposites for Osteoarthritis Treatment
Authors: Kim Hye Jin , Cho Hui Bang , Kim Hye-Ryoung , Lee Sujeong , Park Ji-in , Park Keun-Hong 
Published: October 2023

Abstract
Photoporation techniques based on plasmonic nanoparticles such as gold nanoparticles have been extensively studied for the intracellular delivery of substances via cell membrane disruption. However, the clinical application of AuNP is challenging due to its absorption in the 500 nm region of the light spectrum. To overcome this challenge, upconversion nanoparticles were employed to stimulate AuNP at NIR wavelengths. posAuNP@UCNPs nanocomposites were produced by coating 30 nm UCNPs on 80 nm AuNPs using DOPA-PEI, which were then irradiated with 980 nm NIR light to facilitate their intracellular delivery. TEM and DLS confirmed that posAuNP and UCNP combine to form nanocomposites. Additionally, multiphysics simulation was used to analyze the distribution of the posAuNP electric field based on morphological differences that change as the UCNP ratio increases. Next, effective LED irradiation conditions were established by applying upconverting-photon quenching-mediated perforation influx to C28/I2 cells as suspensions or spheroids. posAuNP@UCNP nanocomposites were confirmed to be effective for the delivery of baricitinib as a treatment for osteoarthritis in a three-dimensional osteoarthritis model. Finally, chondrocyte differentiation was induced through intracellular delivery of baricitinib using posAuNP@UCNPs. The findings suggest that posAuNP@UCNPs have great potential as a tool for non-invasive drug delivery via UCPPin.
Analysis of Influencing Factors of HPV Vaccination Willingness of Female Sex Workers in Urban Entertainment Venues Based on IMB Model in Guangxi, China
Authors: HE Zhiyan , WEI Junhong , JIANG Jianming , HU Rui , QI Guangzi 
Published: October 2023

Abstract
Background
To investigate the willingness of female sex workers in urban entertainment venues in Guangxi, China to vaccinate against HPV and its influencing factors.
Methods
From August 15 to October 15, 2022, the intentional sampling method was used to select female sex workers in commercial sex trading entertainment venues in an urban area of Guangxi as the respondents of the study for the questionnaire survey. The questionnaire based on the information-motivation-behavior skills (IMB) model collected the basic information, HPV and HPV vaccine-related information and cognition, vaccination motivation, behavioral skills, and willingness to vaccination of the respondents. A multi-factor logistic regression model was used to analyze the influencing factors of the HPV vaccination willingness of the respondents.
Results
Among the 921 respondents, 712 (77.31%) were willing to receive HPV vaccination. The higher the level of awareness of HPV and HPV vaccine-related information, the stronger the motivation for HPV and HPV vaccination, and the higher the behavioral skills score, the higher the willingness of female sex workers in entertainment venues to receive HPV vaccination (P < 0.001). Female sex workers in entertainment venues with lower grades [OR(95% CI) = 0.693 (0.539, 0.891), P = 0.004] were more reluctant to receive HPV vaccination; those who favored the vaccines as effective in preventing disease [OR(95% CI) = 2.144 (1.449, 3.174), P < 0.001], had heard of HPV vaccine [OR( 95% CI) = 2.105 (1.451, 3.054), p < 0.001], being able to perceive the benefits of HPV vaccination [OR(95% CI) = 1.134 (1.045, 1.230), p = 0.002] and acquiring more behavioral skills i.e. self-decision for HPV vaccination [OR(95% CI) = 1.130 (1.008, 1.267), P = 0.036] and self-efficacy [OR (95% CI) = 1.135 (1.081, 1.191), P < 0.001] preferred to receive HPV vaccination.
Conclusion
Female sex workers in urban entertainment venues in Guangxi, China, have relatively high willingness to vaccinate against HPV, and should focus on introducing the benefits of primary prevention measures such as HPV vaccine to individuals and behavioral skills of HPV vaccination, so as to improve the willingness and vaccination rate of HPV vaccine.
Efficiency of fixed-ratio combination glucagon-like peptide receptor agonist and basal insulin in type 2 diabetes on different therapeutic regimen
Authors: Risovic Ivona , Djekic Danijel , Bojic Mirjana , Markovic Aleksandra , Dojcinovic Tamara , Vlatkovic Vlastimir , Dobrovoljski Daniela 
Published: October 2023

Abstract
Fixed ratio combination of basal insulin and a glucagon-like peptide-1 receptor agonist (GLP-1RA) offers another option to patients with type 2 diabetes (T2DM) with inadequate glycemic control, especially for those desiring simplified method of treatment intensification in order to improve adherence. There are two types of fixed-ratio combinations: insulin degludec/liraglutide (iDergLira) and insulin glargine/lixisenatide (iGlarLixi). The aim of this study was to evaluate efficacy of intesifying the treatment with fixed ratio combination basal insulin and GLP-1 RA in patients inadequatly controlled on different therapeutic regimen. The study included 200 patients with uncontrolled T2DM, who were divided into four equal groups based on the therapeutic regimen before study: oral diabetic agents or metformin and basal insulin or metformin and GLP 1RA or premix insulin. Our results showed that fixed ratio combination showed better efficiency of on glycemic control in patients previously treated with oral agents and premix insulin, while patients previously treated with premix insulin have had the greatest benefit regarding weight change. We found no difference in glycemic control between iDegLira and iGlarLixi, but significant difference in weight reduction between iDegLira and iGlarLixi.
Effectiveness evaluation of the postnatal care intervention ‘Look! - your baby is talking to you’: A complex intervention trial aiming to reduce depressive symptoms among vulnerable mothers
Authors: Ejlertsen Camilla , Brødsgaard Anne , Kristensen Ingeborg Hedegaard , Urhoj Stine Kjaer , Andersen Anne-Marie Nybo 
Published: October 2023

Abstract

Background
It is widely acknowledged that the postnatal period is crucial in creating a healthy relationship between infant and parent. The mental health of the mother challenges the prerequisites for this process. This study aimed to evaluate whether a short-term intervention delivered to vulnerable mothers during the post-partum stay in hospital reduced the symptoms of depression and impaired well-being three months post partum.
Methods
All new mothers with a history of maternal depression and/or anxiety were invited to participate in a complex intervention trial where they received either care as usual (n=65) or, after a period where health care professionals were trained, were allocated to the intervention (n=57). Background characteristics and baseline data were collected using online questionnaires 48-72 hours post-partum, and the mothers were followed up regarding mental health three months post-partum.
The intervention took place during the planned five-day stay post-partum at the hospital. It consisted of three Newborn Behavioral Observation sessions and three family conversations with trained healthcare professionals, one of which was a meeting including the health visitor from the municipality to improve the transition to the primary healthcare sector. The primary outcome measure was maternal depressive symptoms (Edinburgh Postnatal Depression Scale), and secondary well-being outcome measures were maternal stress (Parental Stress Scale) and Family function (ICE-EFFQ). Differences between the intervention and the non-intervention group were estimated using Poisson and linear regression analyses.
Results
Mothers in the intervention group showed a small, however not statistically significant, reduction in maternal depressive symptoms three months post-partum (OR 0.80 95% CI 0.36-2.00) when compared to mothers in the non-intervention group. No significant differences between the intervention and non-intervention were found regarding the well-being outcomes, although the results pointed towards an improvement in the intervention group. The observation that all mothers with depressive symptoms in the intervention group participated until the end of follow-up suggests that these mothers found the intervention meaningful.
Conclusions
In this short-term and easy-to-implement intervention project ‘Look-your baby is talking to you’, maternal depressive symptoms three months after birth were slightly, however not statistically significantly, decreased in the intervention group compared to the non-intervention group.
Impact of High-Speed Handpiece Noise- Induced Dental Anxiety on Heart Rate: Analyzing Experienced and Non- Experienced Patients - A Comparative Study
Authors: Ghaffar Raahim Salman Abdul , Sheikh Mahnoor , Kidwai Muneeza , Sanaullah Anas , Salman Mousab , Ilyas Anum , Ahmed Naseer , lal Abhishek 
Published: October 2023

Abstract
Background: Dental anxiety is very much common among the patients and could be due to different factors like the behavior of the dentist, past experiences, Needle phobia, or word of mouth from other patients. According to recent studies, a strong association between sound and anxiety has been found, so this observational study has been conducted to find out the link between the activation of anxiety with the sound of a handpiece between experienced patients, who have already gone through the dental treatments and non-experienced patients.
METHODS Total of 297 participants were part of this study. These participants were divided into 2 groups according to the experienced and non-experienced dental patients. The researcher first filled out the CORAH anxiety form to mark the anxiety level of the patients, and then noted the readings of the heart rate in 3 intervals which were before during, and after the treatment with the pulse oximeter. Later the data was analysed using the spss paired t-test.
RESULTS: Results show that patients in group 1 who have gone through the dental treatment before were less anxious and had a lesser effect on their heart rate than the patient who were having the treatment for the first time who were in group 2. Another interesting factor was noticed that in both the groups female were found to be more anxious than male participants. Participants with younger age were found to be more anxious than older age patient in both groups
CONCLUSIONS: The sound of the handpiece can provoke anxiety in the patient, affecting the heart and increasing the heart rate. Participants who were experienced were found to be less anxious than the participants who were inexperienced