bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing
Authors: Chen Xiaohang , Song Haoyue , Song Kun , Zhang Yuan , Wang Jia , Hong Jinjia , Xie Qingpeng , Zhao Jing , Liu Meixian , Wang Xing 
Published: February 2024

AbstractThe dressing that promotes scarless healing is essential for both normal function and aesthetics after a wound. With a deeper understanding of the mechanisms involved in scar formation during the wound healing process, the ideal dressing becomes clearer and more promising. For instance, the yes‐associated transcriptional regulator (YAP) has been extensively studied as a key gene involved in regulating scar formation. However, there has been limited attention given to pectolinarin, a natural flavonoid that may exhibit strong binding affinity to YAP, in the context of scarless healing. In this study, we successfully developed a temperature‐sensitive Pluronic@F‐127 hydrogel as a platform for delivering pectolinarin to promote scarless wound healing. The bioactive pectolinarin was released from the hydrogel, effectively enhancing endothelial cell migration, proliferation and the expression of angiogenesis‐related genes. Additionally, a concentration of 20 μg/mL of pectolinarin demonstrated remarkable antioxidant ability, capable of counteracting the detrimental effects of reactive oxygen species (ROS). Our results from rat wound healing models demonstrated that the hydrogel accelerated wound healing, promoting re‐epithelialization and facilitating skin appendage regeneration. Furthermore, we discovered that a concentration of 50 μg/mL of pectolinarin incorporated to the hydrogel exhibited the most favourable outcomes in terms of promoting wound healing and minimizing scar formation. Overall, our study highlights that the significant potential of locally released pectolinarin might substantially inhibit YAP and promoting scarless wound healing.
Nerve growth factor promote osteogenic differentiation of dental pulp stem cells through MEK/ERK signalling pathways
Authors: Cheng Chen , Tang Shuai , Cui Shuyue , Yang Tong , Li Lan , Zhai Mingrui , Wei Fulan , Ding Gang 
Published: February 2024

AbstractNerve growth factor (NGF) and its receptor, tropomyosin receptor kinase A (TrkA), are known to play important roles in the immune and nervous system. However, the effects of NGF on the osteogenic differentiation of dental pulp stem cells (DPSCs) remain unclear. This study aimed to investigate the role of NGF on the osteogenic differentiation of DPSCs in vitro and the underlying mechanisms. DPSCs were cultured in osteogenic differentiation medium containing NGF (50 ng/mL) for 7 days. Then osteogenic‐related genes and protein markers were analysed using qRT‐PCR and Western blot, respectively. Furthermore, addition of NGF inhibitor and small interfering RNA (siRNA) transfection experiments were used to elucidate the molecular signalling pathway responsible for the process. NGF increased osteogenic differentiation of DPSCs significantly compared with DPSCs cultured in an osteogenic‐inducing medium. The NGF inhibitor Ro 08‐2750 (10 μM) and siRNA‐mediated gene silencing of NGF receptor, TrkA and ERK signalling pathways inhibitor U0126 (10 μM) suppressed osteogenic‐related genes and protein markers on DPSCs. Furthermore, our data revealed that NGF‐upregulated osteogenic differentiation of DPSCs may be associated with the activation of MEK/ERK signalling pathways via TrkA. Collectively, NGF was capable of promoting osteogenic differentiation of DPSCs through MEK/ERK signalling pathways, which may enhance the DPSCs‐mediated bone tissue regeneration.
Clinical manifestations, endoscopic findings and outcomes of tonsillar fossa foreign bodies in dogs: seven cases (2020‐2023)
Authors: Boot G. , Petitpre A. , Lamoureux A. 
Published: February 2024

ObjectivesClinical presentation and outcomes of dogs with tonsillar foreign bodies are not described in the literature. The objective of this case series is to describe the presence, clinical manifestations, endoscopic findings and outcomes of foreign bodies in the palatine tonsillar fossa of dogs.Materials and MethodsMedical records of dogs in which tonsillar fossa foreign bodies were removed by endoscopy between 2020 and 2023 at a referral centre were reviewed. Dogs were included if complete medical records were available.ResultsSeven dogs were included. Among all clinical signs reported, acute onset of throat clearing occurred in all dogs. Palatine tonsil enlargement and protrusion from the tonsillar fossa were observed in four of seven dogs. Foreign bodies were removed under endoscopic guidance in all dogs; a vegetal foreign body was found in all but one dog (6/7). Complete resolution of clinical signs was reported in the five dogs for which follow‐up information was available.Clinical SignificanceThe tonsillar fossa is a rare but possible location for foreign bodies in dogs and should be explored even in the absence of macroscopic lesions of the tonsils, since some dogs could have a normal palatine tonsil appearance. Acute throat clearing should raise the suspicion of tonsillar fossa foreign bodies. The prognosis appears good after removal.
Biogenesis of serum HBV RNA and clinical phenomena of serum HBV RNA in chronic hepatitis B patients before and after receiving nucleos(t)ide analogues therapy
Authors: Wu Liandong , Yang Zhenggang , Zheng Min 
Published: February 2024

AbstractThere are estimated 300 million people afflicted with chronic hepatitis B (CHB) worldwide. The risk of liver cirrhosis and hepatocellular carcinoma (HCC) increases considerably with chronic hepatitis B infection. While current therapeutics are effective in controlling hepatitis B virus (HBV) infection and disease progression, a cure for HBV infection remains unattainable due to an intranuclear replicative intermediate known as covalently closed circular DNA (cccDNA). It has recently been shown that serum HBV RNA is a non‐invasive biomarker that reflects cccDNA transcriptional activity. This review provides a comprehensive overview and the latest updates on the molecular characteristics and clinical significance of serum HBV RNA, such as species of serum HBV RNA, forms of serum HBV RNA carriers and predictive value for relapses in CHB patients after nucleos(t)ide analogues (NAs) discontinuation and development of liver fibrosis and HCC. Furthermore, we summarize standardized assays for testing serum HBV RNA, the dynamic changes of serum HBV RNA levels in treatment‐naïve CHB patients and those under NAs therapy, as well as the host and viral influencing factors of serum HBV RNA levels. Finally, we discuss the future perspectives in studies of serum HBV RNA.
Behaviour change communication to improve complementary feeding practices in Ethiopia: Couples' beliefs concerning paternal involvement in childcare
Authors: Han Yaeeun , Hoddinott John , Kim JiEun , Pelletier David 
Published: February 2024

AbstractAn important cause of stunting is limited consumption of complementary foods, in terms of both quantities and nutrients. Although existing studies show a positive association between fathers' engagement and children's diet, programmes designed to improve complementary feeding practices often only target mothers. In response to this, maternal behaviour change communication (BCC), paternal BCC and food voucher programmes were designed and implemented in Ethiopia using a clustered randomized controlled trial design. The paternal BCC programme included gender‐equal messages to increase fathers' participation in childcare, household labour and decision making. The research reported in this paper is an examination of the BCC programmes, characterizing the behavioural, normative and control beliefs of both mothers and fathers in BCC households compared to those in control households. In this study, a total of 40 participants were included, with 13 mother–father pairs in the BCC + food voucher group, and seven pairs in the control group. Each participant was interviewed separately. We found that BCC mothers showed more gender‐equal tendencies than the control mothers despite being more rural in location. By contrast, the beliefs of BCC and control fathers were similar overall, suggesting men are more resistant to gender‐equal BCC. More work is needed to develop and test effective methods for changing fathers' beliefs and practices.
Renal transplants increase in size and function in keeping with compensatory renal hypertrophy
Authors: Lee Chiang Sheng , Li Jordan Y Z , Juneja Rajiv , Ullah Shahid , van der Jeugd Jane , Gleadle Jonathan M. 
Published: February 2024

AbstractAimTo evaluate changes in allograft kidney length in renal transplant recipients and the relationship with estimated glomerular filtration rate (eGFR).MethodsThis single‐centre retrospective study of renal transplant recipients was conducted at Flinders Medical Centre (FMC) from January 2007 to June 2020. Donor and recipient details, renal allograft length from transplant ultrasounds at 0, 1, 3, 6 and 12 months were collected. The association between compensatory renal hypertrophy (CRH) and eGFR and its magnitude was analysed using multivariate multilevel mixed‐effects linear regression models.ResultsA total of 183 renal transplant recipients were studied. 100 of 175 recipients (62.9%) demonstrated an increase in renal length defined as any increase in maximal longitudinal diameter on serial ultrasounds. Twenty‐three recipients (13.1%) had no change in transplant length and 42 recipients (24%) had a decrease in length. The mean increase in kidney length over the first 12 months was 0.57 cm. Ninety of 156 (57.7%) recipients with a renal ultrasound within a month post‐transplant demonstrated a mean increase kidney length of 0.3 cm. Multivariate analysis demonstrated that eGFR increased by 2.5 mL/min/1.73 m2 (95% CI 0.72– 4.4; p = .006) with every 1 cm increase in kidney length. Absolute changes in kidney length did not demonstrate any statistically significant correlation with eGFR in both complete case and multiple imputation analysis.ConclusionAn increase in transplant kidney length is common in renal transplant recipients and is associated with enhanced eGFR. However, further studies need to be performed to study the association of absolute change in kidney length and eGFR.
The China–Iran strategic deal and CPEC: Navigating the influence of pragmatic balancing in China's relations with Iran and Pakistan
Authors: Hussain Mehmood , Jamali Ahmed Bux , Nisar Rana Danish , Omar Abdulfattah 
Published: February 2024

AbstractThis article argues that China is advancing its relations with Iran and Pakistan based on pragmatic balancing, a key component of soft balancing, to safeguard and advance national self‐interest. China's relationship with Pakistan is based on shared interests to counter the Indian hegemony in South Asia and end the Chinese energy security dilemma through an overland route of the China–Pakistan Economic Corridor (CPEC). The bilateral relationship has enhanced and expanded over the years, despite steep transformations in regional geopolitics. Meanwhile, abundant Iranian energy reserves and Tehran's willingness to support China against the United States in the Middle East encouraged the Chinese leadership to sign a strategic deal with no significant ramifications for Pakistan and CPEC. The fair‐weather friendship between China and Iran remains limited in scope, often suspending cooperation in various domains, including energy and defense. Nonetheless, an all‐weather friendship between China and Pakistan sustained the collaboration of security, economy, and diplomacy, meticulously representing sincerity and cordiality.Related ArticlesBishwakarma, Jham Kumar, and Zongshan Hu. 2021. “Problems and Prospects for the South Asian Association for Regional Cooperation (SAARC).” Politics & Policy 50(1): 154–79. https://doi.org/10.1111/polp.12443.Singh, Amit, and Amit Sarwal. 2017. “Paraspara, Encounters, and Confluences: India's Soft Power Objective in the Indo‐Pacific Region.” Politics & Policy 45(5): 733–61. https://doi.org/10.1111/polp.12222.Zaidi, Syed Muhammad Saad. 2020. “American Global Supremacy under Threat? The Chinese Factor.” Politics & Policy 49(2): 502–28. https://doi.org/10.1111/polp.12402.
Dyadic, biobehavioral, and sociocultural approaches to romantic relationships and health: Implications for research, practice, and policy
Authors: Shrout M. Rosie , Wilson Stephanie J. , Farrell Allison K. , Rice TeKisha M. , Weiser Dana A. , Novak Joshua R. , Monk J. Kale 
Published: February 2024

AbstractRomantic relationships are a key health determinant. Partners influence each other's psychological, behavioral, and biological trajectories in ways that can foster health and longevity or fuel disease risk and early mortality. A romantic relationship's health impact is considerable yet has historically garnered limited recognition from government agencies, healthcare providers, and policymakers. World‐wide public health organizations are increasingly attending to the importance of social connection and health and calling for action and intervention to improve social connection. In this review, we identify key areas to act on this call and advance research, practice, and policy on romantic relationships and health: dyadic effects in how partners influence each other's health; the socio‐historical context and systems of social stratification; and couple‐level prevention, intervention, and health‐promotion efforts. We connect these perspectives and offer next steps to further establish romantic relationships as a public health priority and target for policy and programming that foster social connection and health.
Yield reduction caused by elevated temperatures and high nitrogen fertilization is mitigated by SP6A overexpression in potato (Solanum tuberosum L.)
Authors: Koch Lisa , Lehretz Günter G. , Sonnewald Uwe , Sonnewald Sophia 
Published: February 2024

SUMMARYPotatoes (Solanum tuberosum L.) are a fundamental staple for millions of people worldwide. They provide essential amino acids, vitamins, and starch – a vital component of the human diet, providing energy and serving as a source of fiber. Unfortunately, global warming is posing a severe threat to this crop, leading to significant yield losses, and thereby endangering global food security. Industrial agriculture traditionally relies on excessive nitrogen (N) fertilization to boost yields. However, it remains uncertain whether this is effective in combating heat‐related yield losses of potato. Therefore, our study aimed to investigate the combinatory effects of heat stress and N fertilization on potato tuber formation. We demonstrate that N levels and heat significantly impact tuber development. The combination of high N and heat delays tuberization, while N deficiency initiates early tuberization, likely through starvation‐induced signals, independent of SELF‐PRUNING 6A (SP6A), a critical regulator of tuberization. We also found that high N levels in combination with heat reduce tuber yield rather than improve it. However, our study revealed that SP6A overexpression can promote tuberization under these inhibiting conditions. By utilizing the excess of N for accumulating tuber biomass, SP6A overexpressing plants exhibit a shift in biomass distribution towards the tubers. This results in an increased yield compared to wild‐type plants. Our results highlight the role of SP6A overexpression as a viable strategy for ensuring stable potato yields in the face of global warming. As such, our findings provide insights into the complex factors impacting potato crop productivity.
Magnetic resonance and computed tomographic imaging characteristics and potential molecular mechanisms of feline meningioma associated calvarial hyperostosis
Authors: Edwards Michael R. , Garcia Mora Josefa K. , Fowler Kayla M. , Vezza Christina , Robertson John L. , Daniel Gregory B. , Shinn Richard L. , Parker Rell L. , Werre Stephen R. , Rossmeisl John H. 
Published: February 2024

AbstractMeningiomas are the most common feline primary brain tumours, and calvarial hyperostosis (CH) is frequently documented in association with this neoplastic entity. The clinical significance of and mechanisms driving the formation of CH in cats with meningiomas are poorly understood, although tumour invasion into the skull and tumour production of cytokines and enzymes have been implicated as causes of CH in humans. This retrospective study investigated relationships between signalment, MRI or CT imaging features, histopathologic tumour characteristics, alkaline phosphatase (ALP) isoenzyme concentrations, tumour expression of matrix metalloproteinases (MMP)‐2, MMP‐9, and interleukin‐6 (IL‐6), and progression free survival times (PFS) following surgical treatment in 27 cats with meningiomas with (n = 15) or without (n = 12) evidence of CH. No significant differences in breed, age, sex, body weight, tumour grade, tumour volume, peritumoral edema burden, ALP isoenzyme concentrations, tumour Ki‐67 labelling indices or MMP‐2 or MMP‐9 expression and activity, or PFS were noted between cats with or without CH. There was a trend towards higher serum (p = .06) and intratumoral (p = .07) concentrations of IL‐6 in cats with CH, but these comparisons were not statistically significant. Histologic evidence of tumour invasion into bone was observed in 5/12 (42%) with CH and in no (0/6) cats without CH, although this was not statistically significant (p = .07). Tumour invasion into bone and tumour production of IL‐6 may contribute to the formation of meningioma associated CH in cats, although larger studies are required to further substantiate these findings and determine their clinical relevance.
Mavintramycin A is a promising antibiotic for treating
Mycobacterium avium
complex infectious disease
Authors: Hosoda Kanji , Koyama Nobuhiro , Shigeno Satoru , Nishimura Tomoyasu , Hasegawa Naoki , Kanamoto Akihiko , Ohshiro Taichi , Tomoda Hiroshi 
Published: February 2024

ABSTRACT

Mycobacterium avium
complex (MAC) is a serious disease that is mainly caused by infection with the non-tuberculous mycobacteria (NTM),
Mycobacterium avium
and
Mycobacterium intracellulare
. Seven new compounds, designated mavintramycins A–G (
1
–
7
), were isolated along with structurally related compounds, including amicetin (
9
) and plicacetin (
10
), from the culture broth of
Streptomyces
sp. OPMA40551 as anti-MAC compounds that were active against
M. avium
and
M. intracellulare
. Among them, mavintramycin A showed the most potent and selective inhibition of
M. avium
and
M. intracellulare
. Furthermore, mavintramycin A was active against more than 40 clinically isolated
M. avium
, including multidrug-resistant strains, and inhibited the growth of
M. avium
in a persistent infection cell model using THP-1 macrophages. Mavintramycin A also exhibited
in vivo
efficacy in silkworm and mouse infection assays with NTM. An experiment to elucidate its mechanism of action revealed that mavintramycin A inhibits protein synthesis by binding to 23S ribosomal RNA in NTM. Mavintramycin A, with a different chemical structure from those of clinically used agents, is a promising drug candidate for the treatment of MAC infectious disease.
Genome sequences of two isolates of the yeast
Candida zeylanoides
: UCD849 from soil in Ireland, and AWD from an African wild dog
Authors: McLaughlin Richard W. , Hession Conor , Bergin Sean , Cosgrove Aoife , Dowd Andrew , Garvey Niall , Litovskich Gabrielle , Osaigbovo Evita , Popa Denis , Thuku Cynthia , Butler Geraldine , Wolfe Kenneth H. , Byrne Kevin P. 
Published: February 2024

ABSTRACT

We report genome sequences of two new isolates of the budding yeast
Candida zeylanoides
. Strain UCD849 from soil in Ireland was assembled into eight complete chromosomes. Strain AWD from an African Wild Dog in a US zoo was sequenced to draft level. The assemblies are 10.6 Mb and 99.57% identical.
The complete genome sequences of
Thioglobus autotrophicus
strains EF2 and EF3, isolated from an oxycline in Effingham Inlet, British Columbia
Authors: Morris Robert M. , Mino Sayaka 
Published: February 2024

ABSTRACT

Here we provide the complete genome sequences of two chemoautotrophic isolates from the
Thioglobaceae
family of marine gamma-proteobacteria. The genomes were obtained from pure cultures that were initially isolated from Effingham Inlet in 2013 and revived from freezer stocks for whole genome sequencing in 2023.
Snowball: a novel gene family required for developmental patterning of fruiting bodies of mushroom-forming fungi (Agaricomycetes)
Authors: Földi Csenge , Merényi Zsolt , Balázs Bálint , Csernetics Árpád , Miklovics Nikolett , Wu Hongli , Hegedüs Botond , Virágh Máté , Hou Zhihao , Liu Xiao-Bin , Galgóczy László , Nagy László G. 
Published: February 2024

ABSTRACT

The morphogenesis of sexual fruiting bodies of fungi is a complex process determined by a genetically encoded program. Fruiting bodies reached the highest complexity levels in the Agaricomycetes; yet, the underlying genetics is currently poorly known. In this work, we functionally characterized a highly conserved gene termed
snb1
, whose expression level increases rapidly during fruiting body initiation. According to phylogenetic analyses, orthologs of
snb1
are present in almost all agaricomycetes and may represent a novel conserved gene family that plays a substantial role in fruiting body development. We disrupted
snb1
using CRISPR/Cas9 in the agaricomycete model organism
Coprinopsis cinerea. snb1
deletion mutants formed unique, snowball-shaped, rudimentary fruiting bodies that could not differentiate caps, stipes, and lamellae. We took advantage of this phenotype to study fruiting body differentiation using RNA-Seq analyses. This revealed differentially regulated genes and gene families that, based on wild-type RNA-Seq data, were upregulated early during development and showed tissue-specific expression, suggesting a potential role in differentiation. Taken together, the novel gene family of
snb1
and the differentially expressed genes in the
snb1
mutants provide valuable insights into the complex mechanisms underlying developmental patterning in the Agaricomycetes.

IMPORTANCE
Fruiting bodies of mushroom-forming fungi (Agaricomycetes) are complex multicellular structures, with a spatially and temporally integrated developmental program that is, however, currently poorly known. In this study, we present a novel, conserved gene family, Snowball (snb), termed after the unique, differentiation-less fruiting body morphology of snb1 knockout strains in the model mushroom Coprinopsis cinerea. snb is a gene of unknown function that is highly conserved among agaricomycetes and encodes a protein of unknown function. A comparative transcriptomic analysis of the early developmental stages of differentiated wild-type and non-differentiated mutant fruiting bodies revealed conserved differentially expressed genes which may be related to tissue differentiation and developmental patterning fruiting body development.