bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geographical and sex‐specific effects of malathion insecticide selection in African fig fly Zaprionus indianus Gupta, 1970 (Diptera: Drosophilidae)
Authors: de Oliveira Rios Jonathan , Costa Sávio Cunha , da Conceição Galego Luís Gustavo 
Published: December 2023

AbstractThe African fig fly (AFF) Zaprionus indianus Gupta, 1970, is an invasive species that has caused significant damage to several monoculture crops since its introduction in the Brazilian territory, resulting losses for farmers and decrease of fruit productions. A way to manage fruit pests is the chemical control, which could act as a selective pressure against genetic variation in the insect populations. The esterases are detoxifying enzymes that can respond to the selection by insecticides, they are conserved in evolution, and are involved in the hydrolysis of esters, including insecticide degradation. Esterases can be identified by isoenzyme electrophoresis, which is a valuable tool for genetic, biochemical, and ecological studies, particularly in approaches with pest species. This experimental study aimed to evaluate survival and esterase polymorphisms at the Est2 and Est3 loci in three different geographical populations of Z. indianus from Brazil (Aracaju, Uberaba and Florianópolis) after exposition to organophosphate malathion. We identified patterns of sex‐specific genetic variation that indicate a response to insecticide exposition. These results indicated that the insecticide biological response in this species is sex‐dependent, providing relevant information about the AFF‐malathion interaction. This study may contribute to the development of more effective and sustainable management strategies for AFF control in Brazil using genetic and ecological approaches.
Plastic mulches reduce abundance of some arthropods but are not detrimental to pollinators in primocane raspberries
Authors: McIntosh Hanna , Atucha Amaya , Guédot Christelle 
Published: December 2023

AbstractCultural practices modify agroecosystems to prevent or reduce pest outbreaks, but they may be detrimental to beneficial arthropods and non‐target effects are not commonly evaluated. Plastic mulches are an effective management practice for spotted‐wing drosophila (Drosophila suzukii Matsumura) since they increase UV radiance in the plant canopy to reduce larval infestation of fruit. In this 2‐year study, we evaluated how black, white, and metallic plastic mulches impact populations of arthropods, including predators of D. suzukii and raspberry pollinators in Wisconsin. The three plastic mulches did not affect arthropod richness and did not affect the abundance of about 65% of arthropod groups identified. In the raspberry canopy, the black mulch decreased the abundance of Orthoptera and Thysanoptera and increased Orius spp.; the white mulch increased Diptera, Thysanoptera, and Orius spp.; and the metallic mulch decreased Coleoptera, micro‐Hymenoptera, Orthoptera, and Thysanoptera. On the ground, all mulches decreased the abundance of Gryllidae, the black and metallic increased Formicidae, and the white increased Staphylinidae. We reported 10 insect groups visiting raspberry flowers. Bombus impatiens Cresson was the most common, accounting for 82% of flower visits. The plastic mulches did not reduce flower visitation, though the white plastic mulch increased the abundance of B. impatiens compared to the other mulch treatments. Plastic mulches can reduce populations of pests such as D. suzukii and overall have no severe non‐target effects on other arthropods in Wisconsin raspberry.
Lactic acid bacteria and spoilage bacteria: Their interactions in Escherichia coliO157:H7 biofilms on food contact surfaces and implications for beef contamination
Authors: Nan Yuchen , Rodas‐Gonzalez Argenis , Stanford Kim , Nadon Celine , Yang Xianqin , McAllister Tim , Narváez‐Bravo Claudia 
Published: December 2023

AbstractThis research explores the interaction between Shiga toxin‐producing Escherichia coli (STEC) O157:H7 and bacteria species commonly found in beef processing environments, specifically Carnobacterium, Lactobacillus, Comamonas, Raoultella, and Pseudomonas. The study investigated how various environmental conditions impact the formation of biofilms and the ability of O157:H7 to transfer from multispecies biofilm onto beef surfaces. For this purpose, a mixture of lactic acid bacteria (LAB), spoilage bacteria (106 CFU/mL), and E. coli O157 (103 CFU/mL) were combined as follows: LAB (T1): Carnobacterium piscicola + Lactobacillus bulgaricus + O157:H7, an spoilage bacteria (T2): Comamonas koreensis + Raoultella terrigena + O157:H7, an spoilage bacteria (T3): Pseudomonas aeruginosa + C. koreensis strain + O157:H7 and only O157:H7 as control (T4). Multispecies biofilms were developed on thermoplastic polyurethane (TPU) and stainless steel (SS) coupons at 10 and 25°C for 6 days, washed and stored for 6, 30, and 60 days at wet (60%–90% RH) and dry (20%–50%, RH) conditions. To evaluate O157:H7 transfer, beef cubes (3 × 3 × 1 cm) were placed on the coupons, followed by a 50‐g weight (7.35 kPa). The experiment was repeated three times in triplicate for each strain combination. Results demonstrate that biofilms formed at 10°C were generally weaker (less biomass) than those at 25°C. Regardless of temperature, more viable O157:H7 cells were transferred to beef from moist biofilms on TPU surfaces. At 25°C, T3 biofilm exhibited the lowest O157:H7 transfer to beef by 1.44 log10 CFU/cm2 (p < 0.01). At 10°C, none of the multispecies biofilm (T1–T3) affected the number of O157:H7 transfers to beef (p > 0.05). Notably, O157:H7 was not detected on food contact surfaces with 30 and 60‐day‐old dry biofilms (T1–T4). Through enrichment, E. coli O157:H7 was recovered from multispecies biofilms T1, T2, and T3. Findings from this study imply that multispecies biofilms contribute to the persistence of O157:H7 under dry conditions, regardless of temperature. These results underscore the intricate influence of multiple environmental factors—including surface type, biofilm age, humidity, temperature, and the presence of other bacterial species—on the risk of beef contamination facilitated by biofilms.
Yajé como política. Territorio, petróleo y pandemia en los siekopái de la Amazonía ecuatoriana
Authors: García‐Labrador Julián 
Published: December 2023

AbstractThis article shows the political adaptations of the yajé ritual in the Ecuadorian Amazon. It focuses on the siekopái's practices of resistance, negotiation, and self‐affirmation around three contemporary issues: the recognition of their ancestral territory, the demand for reparations for environmental damage resulting from oil exploitation, and the management of care during the COVID‐19 pandemic. We argue that the inclusion of the yajé ritual in these contexts corresponds to a contested shamanic tradition, and that the deployment of its political potential is due to communal reasons and the shamans' negotiating skills in the invisible world. We conclude that the adaptations indicate an institutional flexibility that has allowed the siekopái to survive to this day in a changing and threatened environment.
Mindfulness, awareness, partner caring, and romantic relationship quality: Structural equation modeling
Authors: Park Cheolwoo , Harris Victor W. , Fogarty Kate 
Published: December 2023

AbstractStudies investigating the potential structures underlying the connection between mindfulness and romantic relationship quality are not sufficient and require additional empirical findings. The current study aimed to examine a hypothesized model underlying the association between mindfulness and relationship quality, while investigating the roles of individuals' level of awareness and application of care in a romantic relationship among 199 adult participants in a southeastern region of the United States. Structural equation modeling (SEM) and path analysis were executed to examine the hypothesized structural model, testing a multi‐step pathway as to how mindfulness influences relationship quality. The SEM and path analyses showed a good fit for the present study's hypothesized model, indicating that individuals' mindfulness had a direct influence on their self‐awareness level. Individuals' self‐awareness and application of care mediated the link between mindfulness and relationship quality. Implications for family life educators, dyadic couples researchers, and marriage and family practitioners are discussed.
Increases in anterograde axoplasmic transport in neurons of the hyper‐glutamatergic, glutamate dehydrogenase 1 (Glud1) transgenic mouse: Effects of glutamate receptors on transport
Authors: Lee Phil , Kim Jieun , Choi In‐Young , Pal Ranu , Hui Dongwei , Marcario Joanne K. , Michaelis Mary L. , Michaelis Elias K. 
Published: December 2023

AbstractThe excitatory neurotransmitter glutamate has a role in neuronal migration and process elongation in the central nervous system (CNS). The effects of chronic glutamate hyperactivity on vesicular and protein transport within CNS neurons, that is, processes necessary for neurite growth, have not been examined previously. In this study, we measured the effects of lifelong hyperactivity of glutamate neurotransmission on axoplasmic transport in CNS neurons. We compared wild‐type (wt) to transgenic (Tg) mice over‐expressing the glutamate dehydrogenase gene Glud1 in CNS neurons and exhibiting increases in glutamate transmitter formation, release, and synaptic activation in brain throughout the lifespan. We found that Glud1 Tg as compared with wt mice exhibited increases in the rate of anterograde axoplasmic transport in neurons of the hippocampus measured in brain slices ex vivo, and in olfactory neurons measured in vivo. We also showed that the in vitro pharmacologic activation of glutamate synapses in wt mice led to moderate increases in axoplasmic transport, while exposure to selective inhibitors of ion channel forming glutamate receptors very significantly suppressed anterograde transport, suggesting a link between synaptic glutamate receptor activation and axoplasmic transport. Finally, axoplasmic transport in olfactory neurons of Tg mice in vivo was partially inhibited following 14‐day intake of ethanol, a known suppressor of axoplasmic transport and of glutamate neurotransmission. The same was true for transport in hippocampal neurons in slices from Glud1 Tg mice exposed to ethanol for 2 h ex vivo. In conclusion, endogenous activity at glutamate synapses regulates and glutamate synaptic hyperactivity increases intraneuronal transport rates in CNS neurons.image
Effect of age on metabolomic changes in a model of paclitaxel‐induced peripheral neurotoxicity
Authors: Bonomo Roberta , Canta Annalisa , Chiorazzi Alessia , Carozzi Valentina Alda , Meregalli Cristina , Pozzi Eleonora , Alberti Paola , Frampas Cecile F. , Van der Veen Daan R. , Marmiroli Paola , Skene Debra J. , Cavaletti Guido 
Published: December 2023

AbstractBackground and AimsChemotherapy‐induced peripheral neurotoxicity (CIPN) is one of the most common dose‐limiting side effects of paclitaxel (PTX) treatment. Many age‐related changes have been hypothesized to underlie susceptibility to damage or impaired regeneration/repair after nerve injury. The results of these studies, however, are inconclusive and other potential biomarkers of nerve impairment need to be investigated.MethodsTwenty‐four young (2 months) and 24 adult (9 months) Wistar male rats were randomized to either PTX treatment (10 mg/kg i.v. once/week for 4 weeks) or vehicle administration. Neurophysiological and behavioral tests were performed at baseline, after 4 weeks of treatment and 2‐week follow‐up. Skin biopsies and nerve specimens collected from sacrificed animals were examined for intraepidermal nerve fiber (IENF) density assessment and nerve morphology/morphometry. Blood and liver samples were collected for targeted metabolomics analysis.ResultsAt the end of treatment, the neurophysiological studies revealed a reduction in sensory nerve action potential amplitude (p < .05) in the caudal nerve of young PTX‐animals, and in both the digital and caudal nerve of adult PTX‐animals (p < .05). A significant decrease in the mechanical threshold was observed only in young PTX‐animals (p < .001), but not in adult PTX‐ones. Nevertheless, both young and adult PTX‐rats had reduced IENF density (p < .0001), which persisted at the end of follow‐up period. Targeted metabolomics analysis showed significant differences in the plasma metabolite profiles between PTX‐animals developing peripheral neuropathy and age‐matched controls, with triglycerides, diglycerides, acylcarnitines, carnosine, long chain ceramides, sphingolipids, and bile acids playing a major role in the response to PTX administration.InterpretationOur study identifies for the first time multiple related metabolic axes involved in PTX‐induced peripheral neurotoxicity, and suggests age‐related differences in CIPN manifestations and in the metabolic profile.
An examination of retracted articles in nursing literature
Authors: Nicoll Leslie H. , Carter‐Templeton Heather , Oermann Marilyn H. , Bailey Hannah E. , Owens Jacqueline K. , Wrigley Jordan , Ledbetter Leila S. 
Published: December 2023

AbstractIntroductionThe output of scholarly publications in scientific literature has increased exponentially in recent years. This increase in literature has been accompanied by an increase in retractions. Although some of these may be attributed to publishing errors, many are the result of unsavory research practices. The purposes of this study were to identify the number of retracted articles in nursing and reasons for the retractions, analyze the retraction notices, and determine the length of time for an article in nursing to be retracted.DesignThis was an exploratory study.MethodsA search of PubMed/MEDLINE, the Cumulative Index to Nursing and Allied Health Literature, and Retraction Watch databases was conducted to identify retracted articles in nursing and their retraction notices.ResultsBetween 1997 and 2022, 123 articles published in the nursing literature were retracted. Ten different reasons for retraction were used to categorize these articles with one‐third of the retractions (n = 37, 30.1%) not specifying a reason. Sixty‐eight percent (n = 77) were retracted because of an actual or a potential ethical concern: duplicate publication, data issues, plagiarism, authorship issues, and copyright.ConclusionNurses rely on nursing‐specific scholarly literature as evidence for clinical decisions. The findings demonstrated that retractions are increasing within published nursing literature. In addition, it was evident that retraction notices do not prevent previously published work from being cited. This study addressed a gap in knowledge about article retractions specific to nursing.
Effect of thickened water swallow training in tube‐feeding and dysphagia patients in the acute and early subacute phases of stroke: A quasi‐experimental study
Authors: Su Jianping , Li Yijing , Xu Zhihua , Sun Dan , Zhu Xiangning , Dong Yueyang , He Meng , Bu Buyin , Sun Jiao 
Published: December 2023

AbstractBackgroundThickened water has been widely used in patients with dysphagia who receive oral feeding, but there is little evidence for tube‐feeding patients.ObjectiveTo explore the effects of thickened water swallow training in tube‐feeding and dysphagia patients in the acute and early subacute phases of stroke.MethodsA quasi‐experimental study. Hospitalised patients with acute and early subacute stroke who received tube feeding due to dysphagia were recruited from March to December 2021. Patients assigned to the intervention group (n = 23) received thickened water swallow training three times daily until the feeding tube was removed or they were discharged, and patients in the control group (n = 23) received usual care. The main outcomes were duration of tube feeding and rates of weaning at discharge.ResultsPatients in the intervention group had a shorter tube‐feeding duration (p = .046) and a higher rate of weaning at discharge (p = .017) than those in the control group. Significant interaction effects between time and group were detected regarding quality of life except for the swallowing burden dimension.ConclusionsThickened water swallow training is feasible and effective for stroke patients with tube feeding and can shorten the duration of tube feeding and improve the rates of weaning and quality of life. Healthcare providers in nonrehabilitation units should actively conduct swallowing function intervention training to maximise the potential for acute and early subacute phase rehabilitation.
Effects of solid and aqueous dietary diflubenzuron ingestion on some biological parameters in synthetic pyrethroid‐resistant German cockroach, Blattella germanicaL. (Blattodea: Ectobiidae)
Authors: Oz Emre , Polat Burak , Cengiz Aysegul , Kahraman Sevval , Gultekin Zeynep Nur , Caliskan Cansu , Cetin Huseyin 
Published: December 2023

AbstractCockroaches, widespread pests found in metropolitan areas, are known as vectors of various disease agents, including viruses, fungi and antibiotic‐resistant bacteria, as well as causing allergies in humans. Insect growth regulators have been used in pest management for several decades. These insecticides disrupt insect development and reproduction. Chitin synthesis inhibitors interfere with chitin biosynthesis in insects, causing abortive moulting and mortality, as well as inhibiting egg fertility, and larval hatching in insects. In this research, we evaluated the various effects of diflubenzuron, a chitin synthesis inhibitor, on synthetic pyrethroid‐resistant German cockroach (Blattella germanica L. Blattodea: Ectobiidae), including ootheca production, oothecal viability, ootheca incubation time, the number of nymphs emerging from the ootheca and survivorship of nymphs. The cockroaches were fed diets that contained diflubenzuron, which was added to solid bait (impregnated fish food) and ingestible aqueous bait (impregnated cotton). Three concentrations (0.5%, 1% and 2%) were used in the experiments. As a result, diflubenzuron treatment led to ootheca production ranging from 60% to 100%; statistically, no difference was found between the treatment and the control groups. The number of nymphs emerging from the first and second ootheca was reduced by 40%–100% in the diflubenzuron‐treated groups compared with the control. Nymphs exposed to diflubenzuron‐impregnated solid bait and ingestible aqueous bait experienced mortality exceeding 92.1% and 66.27% within 15 days, respectively. In conclusion, diflubenzuron is a potential insecticide for use in cockroach baits to control B. germanica, as it caused high nymphal and embryonic mortality in the synthetic pyrethroid‐resistant population and decreased the number of nymphs emerging from the ootheca.
The tomato EAR‐motif repressor, SlERF36, accelerates growth transitions and reduces plant life cycle by regulating GA levels and responses
Authors: Garg Rashmi , Mahato Hrishikesh , Choudhury Upasana , Thakur Ravindra S. , Debnath Pratima , Ansari Nasreen G. , Sane Vidhu A. , Sane Aniruddha P. 
Published: December 2023

SummaryFaster vegetative growth and early maturity/harvest reduce plant life cycle time and are important agricultural traits facilitating early crop rotation. GA is a key hormone governing developmental transitions that determine growth speed in plants. An EAR‐motif repressor, SlERF36 that regulates various growth transitions, partly through regulation of the GA pathway and GA levels, was identified in tomato. Suppression of SlERF36 delayed germination, slowed down organ growth and delayed the onset of flowering time, fruit harvest and whole‐plant senescence by 10–15 days. Its over‐expression promoted faster growth by accelerating all these transitions besides increasing organ expansion and plant height substantially. The plant life cycle and fruit harvest were completed 20–30 days earlier than control without affecting yield, in glasshouse as well as net‐house conditions, across seasons and generations. These changes in life cycle were associated with reciprocal changes in expression of GA pathway genes and basal GA levels between suppression and over‐expression lines. SlERF36 interacted with the promoters of two GA2 oxidase genes, SlGA2ox3 and SlGA2ox4, and the DELLA gene, SlDELLA, reducing their transcription and causing a 3–5‐fold increase in basal GA3/GA4 levels. Its suppression increased SlGA2ox3/4 transcript levels and reduced GA3/GA4 levels by 30%–50%. SlERF36 is conserved across families making it an important candidate in agricultural and horticultural crops for manipulation of plant growth and developmental transitions to reduce life cycles for faster harvest.
A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana
Authors: Grützner Ramona , König Kristin , Horn Claudia , Engler Carola , Laub Annegret , Vogt Thomas , Marillonnet Sylvestre 
Published: December 2023

SummaryTransient expression in Nicotiana benthamiana offers a robust platform for the rapid production of complex secondary metabolites. It has proven highly effective in helping identify genes associated with pathways responsible for synthesizing various valuable natural compounds. While this approach has seen considerable success, it has yet to be applied to uncovering genes involved in anthocyanin biosynthetic pathways. This is because only a single anthocyanin, delphinidin 3‐O‐rutinoside, can be produced in N. benthamiana by activation of anthocyanin biosynthesis using transcription factors. The production of other anthocyanins would necessitate the suppression of certain endogenous flavonoid biosynthesis genes while transiently expressing others. In this work, we present a series of tools for the reconstitution of anthocyanin biosynthetic pathways in N. benthamiana leaves. These tools include constructs for the expression or silencing of anthocyanin biosynthetic genes and a mutant N. benthamiana line generated using CRISPR. By infiltration of defined sets of constructs, the basic anthocyanins pelargonidin 3‐O‐glucoside, cyanidin 3‐O‐glucoside and delphinidin 3‐O‐glucoside could be obtained in high amounts in a few days. Additionally, co‐infiltration of supplementary pathway genes enabled the synthesis of more complex anthocyanins. These tools should be useful to identify genes involved in the biosynthesis of complex anthocyanins. They also make it possible to produce novel anthocyanins not found in nature. As an example, we reconstituted the pathway for biosynthesis of Arabidopsis anthocyanin A5, a cyanidin derivative and achieved the biosynthesis of the pelargonidin and delphinidin variants of A5, pelargonidin A5 and delphinidin A5.
The tautomer‐specific excited state dynamics of 2,6‐diaminopurine using resonance‐enhanced multiphoton ionization and quantum chemical calculations
Authors: Gate Gregory , Williams Ann , Boldissar Samuel , Šponer Jiří , Szabla Rafal , de Vries Mattanjah 
Published: December 2023

Abstract2,6‐Diaminopurine (2,6‐dAP) is an alternative nucleobase that potentially played a role in prebiotic chemistry. We studied its excited state dynamics in the gas phase by REMPI, IR‐UV hole burning, and ps pump‐probe spectroscopy and performed quantum chemical calculations at the SCS‐ADC(2) level of theory to interpret the experimental results. We found the 9H tautomer to have a small barrier to ultrafast relaxation via puckering of its 6‐membered ring. The 7H tautomer has a larger barrier to reach a conical intersection and also has a sizable triplet yield. These results are discussed relative to other purines, for which 9H tautomerization appears to be more photostable than 7H and homosubstituted purines appear to be less photostable than heterosubstituted or singly substituted purines.