bluuebunny/crossref_metadata_2025_split huggingface dataset:
Red Blood Cells Inhibit Proliferation and Stimulate Apoptosis in Human Lung Fibroblasts In Vitro
Authors: Fredriksson K. , Stridh H. , Lundahl J. , Rennard S. I. , Skold C. M. 
Published: June 2004

AbstractCell proliferation and apoptosis are both important mechanisms for the regulation of tissue homeostasis. For instance, proliferation is crucial in wound repair, whereas apoptosis is important for removal of damaged cells and resolution of inflammation. Imbalance between cell proliferation and apoptosis can therefore lead to pathological conditions and disease. In inflammatory and fibrotic lung disorders, red blood cells (RBCs) can interact with fibroblasts and connective tissue. In the present study, we therefore hypothesized that the presence of RBCs can affect fibroblast proliferation and apoptosis. Human foetal lung fibroblasts (HFL‐1) were cultured in the presence or absence of purified whole RBCs and RBC‐conditioned media. RBC significantly decreased fibroblast proliferation as determined both by DNA content analysis (Hoechst 33258 staining, P < 0.01; WST‐1, P < 0.001) and BrdU incorporation. After treatment with staurosporine (STS) for 48 h, apoptosis was determined by TUNEL and propidium iodide staining followed by flow cytometry analysis. RBCs augmented STS‐induced apoptosis (median: 46.4%; range 12.0–90.4) compared to control cells (median 26.2%; range 7.1–45.5). Thus, our data indicate that the presence of RBCs affects both fibroblast proliferation and susceptibility to undergo apoptosis. Our findings therefore suggest a role for RBCs in regulating fibroblast homeostasis after tissue injury.
The study of the participation of basement membrane zone antibodies in the formation of the lupus band in systemic lupus erythematosus
Authors: Yang Sen , Gao Yu , Song Yingxue , Liu Jiangbo , Yang Chunjun , Liu Jinli , Wang Zaixing , Zhang Xuejun 
Published: May 2004

AbstractBackground  The lupus band test (LBT) is an important auxiliary method in the diagnosis of systemic lupus erythematosus (SLE), but the mechanism of its formation is still unknown. There are many kinds of autoantibodies, such as basement membrane zone autoantibodies (BMZ‐Abs), in patients with SLE.Aim  To detect whether skin BMZ‐Abs participated in the formation of the lupus band in SLE.Methods  Immunoglobulin G (IgG)‐type BMZ‐Abs in 15 SLE patients were detected by means of immunofluorescence (IF), immunoblotting (IB), and immunoelectron microscopy (IEM).Results  Direct immunofluorescence (DIF) on salt‐split skin showed epidermal fluorescence in 12 of the 15 SLE patients. Two of the 12 patients also showed dermal fluorescence. Indirect immunofluorescence (IIF) on salt‐split skin revealed that, in 12 of the 15 (80%) sera, antibodies were bound to the epidermal roof of the salt‐split skin. IB showed that, in 14 SLE sera, autoantibodies reacted to 230, 200, 180, 130, and 97 kDa epidermal extracts and 75 kDa dermal extracts. Direct (DIEM) and indirect (IIEM) immunoelectron microscopy showed that gold particles were directed to every region of the BMZ, including hemidesmosomes, lamina lucida, lamina densa, and sublamina densa.Conclusions  BMZ‐Abs in SLE sera may participate in the formation of the lupus band.
Broad‐band UVB fiber‐optic comb for the treatment of scalp psoriasis: a pilot study
Authors: Taneja Atul , Racette Ashley , Gourgouliatos Zafirios , Taylor Charles R. 
Published: May 2004

AbstractBackground  Scalp psoriasis is a frustrating condition with limited treatment options. Our objective was to test a new fiber‐optic broad‐band UVB comb device for the treatment of this condition.Methods  Scalp psoriasis on adult subjects was treated three times a week for up to 12 weeks with a fiber‐optic UVB comb. A minimum 50‐cm2 scalp area served as the control. Starting doses were based on the standard minimal erythema dose (MED) with increments up to 20% for subsequent treatments.Results  Fourteen subjects completed the study without sunburn reactions or blisters. Treated sites showed a mean improvement in the modified PASI score of 3.6. The difference in the mean modified PASI scores between the control and treated sites was 3.9 (< 0.0001).Conclusions  This fiber‐optic broad‐band UVB comb can safely and effectively treat scalp psoriasis.
Melanoma epidemic: True or false?
Authors: Flórez Ángeles , Cruces Manuel 
Published: May 2004

AbstractMelanoma incidence has increased dramatically over the last decades in most industrial countries, mainly as a result of the large numbers of early melanomas being diagnosed. Simultaneously, a lack of commensurate change in mortality has been reported, raising the possibility that skin melanoma may have modified its aggressiveness as a result of the increased diagnosis of biologically benign lesions. The main data and controversies arising from the melanoma epidemic are reviewed.
Celecoxib‐induced photoallergic drug eruption
Authors: Yazici Ayca Cordan , Baz Kiymet , Ikizoglu Guliz , Kokturk Aysin , Uzumlu Hale , Tataroglu Canten 
Published: May 2004

AbstractPhotoallergic dermatitis is caused by a photosensitizing substance plus sunlight exposure in a sensitized person. If the photosensitizer is delivered internally, it is called a photoallergic drug reaction. Celecoxib is a new generation non‐steroidal anti‐inflammatory drug and sulfonamide derivative. We report a photoallergic drug eruption associated with the introduction of celecoxib. To our knowledge, this is the first report of photoallergic drug reaction associated with celecoxib.
Tinea faciei, an often deceptive facial eruption
Authors: Lin Richie L. , Szepietowski Jacek C. , Schwartz Robert A. 
Published: May 2004

AbstractTinea faciei is a relatively uncommon superficial dermatophyte infection limited to the glabrous skin of the face. It is usually seen in pediatric patients. This fungus can be found worldwide, but has a predilection for tropical humid climates. Although the infecting organisms and treatments are the same as for other superficial fungal infections, it is frequently misdiagnosed clinically and histologically.
Effect of feeding intensity and time on feed on intramuscular fatty acid composition of Simmental bulls
Authors: Sami A. S. , Augustini C. , Schwarz F. J. 
Published: May 2004

SummarySeventy‐two Simmental bulls, weighing 489 kg initially and approximately 15 months old, were divided into four groups to determine the effects of feeding intensity and time on feed on intramuscular fatty acid (FA) composition. Two groups of 18 bulls each were extensively (E) or intensively (I) fed on maize silage and concentrates with a daily gain of 943 g (E) or 1371 g (I). Half of each group were slaughtered after 100 days (S) or 138 days (L) on feed. In addition to carcass fatness parameters, intramuscular FA composition was also measured. Only small differences in the sum of saturated FA (SFA) percentages were found with 47.7 and 47.5% FA methyl esters (FAME) for SE and LE, respectively, and 48.7% FAME for each of SI and LI. Monounsaturated fatty acids (MUFA) significantly increased with longer, and higher feeding intensity to 44.2% FAME (LI), whereas the other groups had similar contents of 41.9, 42.2 and 42.0% FAME (SE, LE and SI respectively). Polyunsaturated FA (PUFA) decreased with higher feeding intensity to 8.39% (SI) and 6.71% (LI) FAME (p < 0.05) in comparison with 9.48% (SE) and 9.54% (LE). Intensive feeding decreased the ratio of PUFA : SFA to 0.17 (SI) and 0.14 (LI, p < 0.05) in comparison with 0.20 (SE and LE). The mean conjugated linoleic acid (CLA) concentration was 0.41% FAME. Time on feed had only a small effect on the FA composition compared with feeding intensity. No significant relationships were detected between meat quality attributes and the pattern of FA.
Inhibition of ruminal microbial methane production by β‐cyclodextrin iodopropane, malate and their combination in vitro
Authors: Mohammed N. , Lila Z. A. , Ajisaka N. , Hara K. , Mikuni K. , Hara K. , Kanda S. , Itabashi H. 
Published: May 2004

SummaryThe objective of this study was to evaluate the effects of different concentrations of l‐malate (0, 5, 10 and 20 mm), 2‐iodopropane‐β‐cyclodextrin complex (CD‐IP) (0, 0.1, 0.2 and 0.4 mm) and a combination of malate (10 and 20 mm) plus CD‐IP (0.2 and 0.4 mm) on methane production from corn starch. Ruminal fluid was collected from dairy cows, mixed with phosphate buffer (1 : 2) and incubated (30 ml) anaerobically at 38 °C for 6 h with or without additives. Fermentation of corn starch in the presence of malate resulted in an increase (p < 0.05) in pH of the medium, total volatile fatty acid (VFA), total gas production and molar proportion of propionate. Acetate and ammonia‐N concentration were unchanged. Methane production was decreased (p < 0.05) (15.5 to 20.4%). Addition of CD‐IP in corn starch resulted in an increase (p < 0.05) in total VFA and molar proportion of propionate. Acetate, pH and ammonia‐N concentration of the medium were decreased (p < 0.05). Total gas production was unchanged. Methane production was decreased (p < 0.05) (25.2 to 97.1%) and hydrogen production was increased (p < 0.05). Addition of l‐malate to CD‐IP resulted in an increase (p < 0.05) in total VFA, total gas production and molar proportion of propionate. Acetate and ammonia‐N concentration were decreased (p < 0.05). No effects were observed on medium pH. Methane production was decreased (p < 0.05) (49.5 to 97.1%). Hydrogen production was also decreased (p < 0.05) (54.5 to 64.1%) compared with those of CD‐IP alone. Therefore, these additives may be used as supplements to inhibit methane production as well as to improve rumen fermentation and animal performance.
Influence of dietary linseed, fish and coconut oil on growth performance of growing pigs kept on small holdings in central Vietnam
Authors: Nguyen L. Q. , Everts H. , Beynen A. C. 
Published: May 2004

SummaryThe aim of the study was to investigate the effect on growth performance of the addition of 5% of either linseed, fish or coconut oil to the diet of growing pigs kept on small holdings in central Vietnam. The diets were fed on six different farms; there were three animals per treatment per farm. The farmers fed a base diet according to personal choice, but were instructed to use linseed, fish and coconut oil. The diets were fed to the pigs from 70 to 130 days of age. The diets with 5% linseed oil on average contained 2.4 g α‐linolenic acid/MJ of metabolizable energy (ME) and the diets with 5% fish oil on average contained 0.06 and 0.75 g eicosapentaenoic and docosahexaenoic acid/MJ ME, respectively. The relative percentages of docosahexaenoic, eicosapentaenoic, α‐linolenic and linoleic acid in adipose tissue were determined by the intake of the corresponding fatty acids. Dietary linseed oil vs. coconut oil significantly enhanced daily weight gain and vs. fish oil it also stimulated growth, but this effect just failed to reach statistical significance. It is concluded that extra intake of α‐linolenic acid may stimulate growth in growing‐finishing pigs, this effect being independent of the conversion of α‐linolenic acid into eicosapentaenoic and docosahexaenoic acid.
Effects of supplemental L‐carnitine in drinking water on performance and egg quality of laying hens exposed to a high ambient temperature
Authors: Çelik L. B. , Tekeli A. , Öztürkcan O. 
Published: May 2004

SummaryThe present study was conducted to investigate effects of l‐carnitine supplied with drinking water on performance and egg quality of laying hens under high environmental temperature. In the study, 47‐week‐old laying hens (Brown hisex) were divided into two groups (control and treatment) and fed with a standard layer diet. Treatment group was received 50 p.p.m l‐carnitine with drinking water for an 8‐week period. Throughout the study, 8 h hot (35–37 °C) and 16 h thermoneutral (20–22 °C) environmental temperature regime was employed daily. The results showed that l‐carnitine supplementation affected some egg quality characteristics of layers under high environmental temperature. Relative albumen weight and height were increased (p < 0.05) by supplemental l‐carnitine. Live weight gain, feed intake, egg mass, egg weight, yolk weight, shell weight, yolk index, egg‐shape index, yolk colour score and shell thickness were not affected (p < 0.05) by l‐carnitine. It is concluded that l‐carnitine supplementation in laying hens could have potential to improve albumen quality under high environmental temperature.
The study of introgressive lines of Triticum aestivum × Aegilops speltoides by in situ and SSR analyses
Authors: Adonina I. G. , Salina E. A. , Efremova T. T. , Pshenichnikova T. A. 
Published: June 2004

AbstractFluorescence in situ hybridization (FISH) with a genome‐specific repeat, Spelt1, and wheat simple sequence repeat (SSR) markers were used to analyse the chromosome constitution of two Triticum aestivum×Aegilops speltoides introgressive lines. The lines 170/98i and 178/98i carried one and two subtelomeric regions of Ae. speltoides (per haploid genome), respectively, marked by Spelt1 repeats according to FISH data. SSR analysis detected homoeologous substitution of wheat chromosome 7D with Ae. speltoides chromosome 7S in the lines 178/98i and 170/98i as well as the assumed terminal translocation in the short arm of chromosome 3A in the line 178/98i. Anthocyanin pigmentation of the coleoptiles was found in the lines 170/98i and 178/98i and resulted from the 7S (7D) substitution. It was demonstrated that Spelt1 could be effectively used for the rapid identification (without DNA isolation) of terminal translocations of T. aestivum×Ae. speltoides introgressive lines as well as for further analysis of the stability of the hybrid plants.
Inheritance of resistance to spot blotch caused by Bipolaris sorokiniana in spring wheat
Authors: Joshi A. K. , Kumar S. , Chand R. , Ortiz‐Ferrara G. 
Published: June 2004

AbstractThree F1 progenies and their families in the segregating generations (F3, F4, F5 and F6), obtained after crossing resistant × susceptible wheat genotypes were studied in the field to determine the genetics of resistance to spot blotch caused by Bipolaris sorokiniana. Spot blotch scores in the F1 generation showed absence of dominance. Individually threshed F2 plants were used to advance the generations. Progenies (200‐250) of resistant genotypes Acc. No. 8226, Mon/Ald, Suzhoe#8 crossed with susceptible ‘Sonalika’ were evaluated in the F3, F4, F5 and F6 generations under induced epiphytotic conditions. Based on disease score distribution in individual progeny rows, F3 progenies were grouped into four classes: homozygous resistant, homozygous susceptible, segregating resistant and segregating susceptible. Resistance appeared to be under the control of three additive genes. The presence of three genes was also noted in the distribution of F4 and F5 lines. In the case of F6 progeny rows, both quantitative and qualitative models were used to estimate the number of segregating genes based on a 2‐year trial. It appeared that resistance to spot blotch was controlled by the additive interaction of more than two genes, possibly only three.
Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus)
Authors: Lu G. Y. , Yang G. S. , Fu T. D. 
Published: June 2004

AbstractRs1046AB is a genic male sterile two‐type line in rapeseed that has great potential for hybrid seed production. The sterility of this line is conditioned by the interaction of two genes, i.e. the dominant genic male sterility gene (Ms) and the suppressor gene (Rf). The present study was undertaken to identify DNA markers for the Ms locus in a BC1 population developed from a cross between a male‐sterile plant in Rs1046AB and the fertile canola‐type cultivar ‘Samourai’. Bulked segregant analysis was performed using the amplified fragment length polymorphism (AFLP) methodology. From the survey of 480 AFLP primer combinations, five AFLP markers (P10M13350, P13M8400, P6M6410, E7M1230 and E3M15100) tightly linked to the target gene were identified. Two of them, E3M15100 and P6M6410, located the closest, at either side of Ms at a distance of 3.7 and 5.9 cM, respectively. The Ms locus was subsequently mapped on linkage group LG10 in the map developed in this laboratory, adding two additional markers weakly linked to it. This suite of markers will be valuable in designing a marker‐assisted genic male sterility three‐line breeding programme.
Effect of genetic background on the target fatty acids of acyl‐ACP thioesterase transgenes in Brassica napus
Authors: Tang J. , Scarth R. 
Published: June 2004

AbstractAcyl‐acyl carrier protein (ACP) thioesterase (TE) is involved in the biosynthetic fatty acid pathway of plants. Conventional canola lines transformed individually with the bay‐TE (Uc FatB1), elm‐TE (Ua FatB1), nutmeg‐TE (Mf FatB1) or Cuphea‐TE transgene (Ch FatB1), produce seed oil with modified fatty acid compositions. This study assessed the effects of genetic background, cytoplasm, maternal parent, and interaction of different TE transgenes, on the target fatty acids using F1 seeds and double haploid (DH) lines. The F1 seeds were produced by crossing four TE transgenic parental lines and three non‐transgenic cultivars with distinct fatty acid compositions. The DH lines were developed from microspores of F1 plants. DH lines from different crosses showed that genetic background does not have an effect on the relative levels of the target fatty acids of the same TE, indicating the stability of the substrate specificity of the TE within canola. However, significant effects of genetic background on the content of the major target fatty acids, lauric acid (C12:0) or palmitic acid (C16:0) depending on the TE, were observed. Expression of the TE in low erucic acid (C22:1) genotypes resulted in higher target fatty acid levels than expression in high C22:1 genotypes. Reciprocal crosses showed maternal effects, but not cytoplasmic effects. In addition, co‐expression of two different TE transgenes in the same seeds was observed. These results indicate the importance of selection for appropriate genetic backgrounds in order to maximize the expression of the target fatty acids of TE transgenes, and also indicate potential uses of TE co‐expression in modifying canola seed oil.
Spanish spelt: a separate gene pool within the spelt germplasm
Authors: Elía M. , Moralejo M. , Rodríguez‐Quijano M. , Molina‐Cano J. L. 
Published: June 2004

AbstractThe renewed interest in spelt (Triticum spelta L.) for wheat improvement programmes requires the study of the available genetic diversity. The purpose of this study was to assess genetic diversity within a Spanish spelt collection. Sixty‐six Spanish spelt accessions, 19 accessions of T. spelta and T. macha from different origins, three bread wheat cultivars (T. aestivum) and one accession of T. dicoccum were screened using simple sequence repeats (SSRs). The diversity observed within the Spanish group was comparable with that observed in the other wheat varieties, despite their broader geographical diversity. Indeed, the highest polymorphic information content value calculated with SSRs for Spanish material (0.90) is similar to that observed for the other wheat varieties (0.98). Principal component analysis explained 46.5% of the cumulative variation and confirmed the Spanish accessions as a separate group. This study showed the Spanish spelt collection to be a variable and unique genetic resource for wheat and spelt breeding programmes.
Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two‐pair primers
Authors: Domon E. , Yanagisawa T. , Saito A. , Takeda K. 
Published: June 2004

AbstractA high‐throughput single nucleotide polymorphism (SNP) genotyping procedure was developed to select amylose‐free barley mutants whose waxy genes had a C‐ to T‐base substitution in exon 5, which converted Gln‐89 of the wild‐type gene into a termination codon. An F2 population carrying an amylose‐free waxy gene was checked for segregation. Polymerase chain reaction with confronting two‐pair primers (PCR‐CTPP) produced allele‐specific PCR products that have different sizes and are inherited in a co‐dominant manner. Two alleles of the barley waxy gene with SNP were correctly identified in parental strains using the PCR‐CTPP procedure. Segregation of the SNP as detected by PCR‐CTPP in an F2 population fitted the expected 1:2:1 ratio. The PCR‐CTPP procedure can provide a time saving and cost‐effective alternative to derived cleaved amplified polymorphic sequence in marker‐assisted selection.
Genetics, fertility behaviour and molecular marker analysis of a new TGMS line, TS6, in rice
Authors: Latha R. , Thiyagarajan K. , Senthilvel S. 
Published: June 2004

AbstractThe thermosensitive genic male sterility (TGMS) system has great potential for revolutionizing hybrid rice production through simple, less expensive and more efficient seed production technology. For the successful utilization of this novel male sterility system, knowledge of the breeding and fertility behaviour of a TGMS line is essential. In this study, the fertility transformation behaviour, the critical fertility and sterility temperatures and the mode of inheritance of male sterility were studied for a new TGMS line, TS6, identified at Tamil Nadu Agricultural University, Coimbatore, India. The pollen and spikelet fertilities recorded on plants raised at fortnightly intervals revealed that this line was completely sterile for 78 consecutive days (35/22 to 32/23°C, maximum/minimum temperatures) and reverted to fertile when the temperature was 30/18°C. It remained fertile continuously for 69 days and the maximum pollen and spikelet fertilities recorded were 75 and 70%, respectively. The fertility was highly influenced by daily maximum temperature followed by average and minimum temperatures. It was not influenced by relative humidity, sunshine hours or photoperiod. The critical temperature inducing sterility and fertility was 26.7 and 25.5°C, respectively. The male sterility in TS6 was inherited as a monogenic recessive in the F2 and BC1 populations of TS6 × MRST9 as well as TS6 × IR68281B. Using bulked segregant analysis on an F2 population of TS6 × MRST9, an RAPD marker, OPC052962, was identified to be associated with TGMS in TS6.
Identification of quantitative trait loci underlying milling quality of rice (Oryza sativa) grains
Authors: Li Z. F. , Wan J. M. , Xia J. F. , Zhai H. Q. , Ikehashi H. 
Published: June 2004

AbstractMilling quality of rice grains is important to both producers and consumers. In this study, quantitative trait loci (QTLs) controlling brown rice rate (BR), milled rice recovery (MR) and head rice recovery (HR) were analysed by composite interval mapping over 2 years using 98 backcross inbred lines (BILs). A total of 12 QTLs for the three traits were detected, of which five were for BR, four for MR and three for HR. The proportion of phenotypic variation explained by individual QTLs ranged from 7.5 to 19.9%, and additive effects contributed by a single QTL accounted for 0.46 to 2.34% of the variation. QTL‐by‐environment interactions were observed by comparing QTL mapping of the same population grown in two consecutive years. Three of five QTLs for BR and two of four QTLs for MR were detected in 2 years, and all three QTLs for HR were detected in 1 year only. BR was significantly correlated with MR, and all four QTLs of MR were located in the same regions as those of BR. This indicated that QTLs for highly correlated traits could often be detected in the same interval.