bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea
Authors: Wang Xintong , Meng Xianghong , Dong Yanlong , Song Chunhua , Sui Fengyang , Lu Xinxin , Mei Xiaoxue , Fan Yawen , Liu Yan 
Published: January 2024

AbstractBackgroundThe increasingly severe salinization of the aquatic environment has led to serious damage to the habitats of aquatic organisms. Benthic diatoms are commonly employed as indicator species for assessing water quality and serve as a reflection of the overall health of the aquatic ecosystem.Nitzschia paleais a common diatom found in freshwater, with high oil content, rapid reproductive rate, and it is a commonly dominant species in various rivers.ResultsThe results showed that after 4 days (d) of saline-alkali stress, the cell density and chlorophyll a content ofNitzschia paleareached their maximum values. Therefore, we selectedNitzschia paleaunder 4 d stress for Tandem Mass Tag (TMT) quantitative proteomic analysis to explore the molecular adaptation mechanism of freshwater diatoms under saline-alkali stress. Totally, 854 proteins were enriched, of which 439 differentially expressed proteins were identified. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and subcellular fractionation analysis revealed that these proteins were mainly enriched in the photosynthesis pathway, citric acid cycle (TCA cycle), fatty acid synthesis, and glutathione cycle.ConclusionsThis study aims to reveal the physiological, biochemical and proteomic mechanisms of salt and alkali tolerance and molecular adaptation ofNitzschia paleaunder different saline-alkali concentrations. This study showed thatNitzschia paleais one candidate of the environmental friendly, renewable bioenergy microalgae. Meantime,Nitzschiapaleareveals for the proteome of the freshwater and provides the basis, it became a model algal species for freshwater diatoms.
Low-temperature methanation of fermentation gas with Ni-based catalysts in a multicomponent system
Authors: Yin Jie , Yao Zihui , Zhao Qizhi , Cheng Shikun , Wang Xuemei , Li Zifu 
Published: January 2024

AbstractA large amount of greenhouse gases, such as carbon dioxide and methane, are released during the production process of bioethanol and biogas. Converting CO2 into methane is a promising way of capturing CO2 and generating high-value gas. At present, CO2 methanation technology is still in the early stage. It requires high temperature (300–400 ℃) and pressure (> 1 MPa), leading to high cost and energy consumption. In this study, a new catalyst, Ni–Fe/Al–Ti, was developed. Compared with the activity of the common Ni/Al2O3 catalyst, that of the new catalyst was increased by 1/3, and its activation temperature was reduced by 100℃. The selectivity of methane was increased to 99%. In the experiment using simulated fermentation gas, the catalyst showed good catalytic activity and durability at a low temperature and atmospheric pressure. Based on the characterization of catalysts and the study of reaction mechanisms, this article innovatively proposed a Ni–Fe/Al–Ti quaternary catalytic system. Catalytic process was realized through the synergism of Al–Ti composite support and Ni–Fe promotion. The oxygen vacancies on the surface of the composite carrier and the higher activity metals and alloys promoted by Fe accelerate the capture and reduction of CO2. Compared with the existing catalysts, the new Ni–Fe/Al–Ti catalyst can significantly improve the methanation efficiency and has great practical application potential.
Isopropanol production via the thermophilic bioconversion of sugars and syngas using metabolically engineered Moorella thermoacetica
Authors: Kato Junya , Matsuo Takeshi , Takemura Kaisei , Kato Setsu , Fujii Tatsuya , Wada Keisuke , Nakamichi Yusuke , Watanabe Masahiro , Aoi Yoshiteru , Morita Tomotake , Murakami Katsuji , Nakashimada Yutaka 
Published: January 2024

Abstract
Background
Isopropanol (IPA) is a commodity chemical used as a solvent or raw material for polymeric products, such as plastics. Currently, IPA production depends largely on high-CO2-emission petrochemical methods that are not sustainable. Therefore, alternative low-CO2 emission methods are required. IPA bioproduction using biomass or waste gas is a promising method.

Results
Moorella thermoacetica, a thermophilic acetogenic microorganism, was genetically engineered to produce IPA. A metabolic pathway related to acetone reduction was selected, and acetone conversion to IPA was achieved via the heterologous expression of secondary alcohol dehydrogenase (sadh) in the thermophilic bacterium. sadh-expressing strains were combined with acetone-producing strains, to obtain an IPA-producing strain. The strain produced IPA as a major product using hexose and pentose sugars as substrates (81% mol-IPA/mol-sugar). Furthermore, IPA was produced from CO, whereas acetate was an abundant byproduct. Fermentation using syngas containing both CO and H2 resulted in higher IPA production at the specific rate of 0.03 h−1. The supply of reducing power for acetone conversion from the gaseous substrates was examined by supplementing acetone to the culture, and the continuous and rapid conversion of acetone to IPA showed a sufficient supply of NADPH for Sadh.

Conclusions
The successful engineering of M. thermoacetica resulted in high IPA production from sugars. M. thermoacetica metabolism showed a high capacity for acetone conversion to IPA in the gaseous substrates, indicating acetone production as the bottleneck in IPA production for further improving the strain. This study provides a platform for IPA production via the metabolic engineering of thermophilic acetogens.
Extracellular vesicles involved in growth regulation and metabolic modulation in Haematococcus pluvialis
Authors: Hu Qunju , Hu Zhangli , Yan Xiaojun , Lu Jun , Wang Chaogang 
Published: January 2024

Abstract
Background
Microalgae-derived extracellular vesicles (EVs), which transfer their cargos to the extracellular environment to affect recipient cells, play important roles in microalgal growth and environmental adaptation. And, they are also considered as sustainable and renewable bioresources of delivery nanocarrier for bioactive molecules and/or artificial drug molecules. However, their molecular composition and functions remain poorly understood.

Results
In this study, isolation, characterization, and functional verification of Haematococcus pluvialis-derived EVs (HpEVs) were performed. The results indicated that HpEVs with typical EV morphology and size were secreted by H. pluvialis cells during the whole period of growth and accumulated in the culture medium. Cellular uptake of HpEVs by H. pluvialis was confirmed, and their roles in regulation of growth and various physiological processes of the recipient cells were also characterized. The short-term inhibition of HpEV secretion results in the accumulation of functional cellular components of HpEVs, thereby altering the biological response of these cells at the molecular level. Meanwhile, continuously inhibiting the secretion of HpEVs negatively influenced growth, and fatty acid and astaxanthin accumulation in H. pluvialis. Small RNA high-throughput sequencing was further performed to determine the miRNA cargoes and compelling details in HpEVs in depth. Comparative analysis revealed commonalities and differences in miRNA species and expression levels in three stages of HpEVs. A total of 163 mature miRNAs were identified with a few unique miRNAs reveal the highest expression levels, and miRNA expression profile of the HpEVs exhibited a clear stage-specific pattern. Moreover, a total of 12 differentially expressed miRNAs were identified and their target genes were classified to cell cycle control, lipid transport and metabolism, secondary metabolites biosynthesis and so on.

Conclusion
It was therefore proposed that cargos of HpEVs, including miRNA constituents, were suggested potential roles in modulate cell physiological state of H. pluvialis. To summarize, this work uncovers the intercellular communication and metabolism regulation functions of HpEVs.
A systematic review and meta-analysis of protozoan parasite infections among patients with mental health disorders: an overlooked phenomenon
Authors: Abdoli Amir , Olfatifar Meysam , Eslahi Aida Vafae , Moghadamizad Zeinab , Samimi Rasoul , Habibi Mohammad Amin , Kianimoghadam Amir Sam , Badri Milad , Karanis Panagiotis 
Published: January 2024

Abstract
Background
Patients with mental disorders have a high risk of intestinal parasitic infection due to poor hygiene practices. Hence, to better clarify this overlooked phenomenon, the current study is conducted to determine the global prevalence of protozoan parasite infections in patients with mental disorders and investigate the associated risk factors.

Methods
Several databases (PubMed, Scopus, Web of Science, ProQuest, and Google Scholar) were searched for papers published until December 2022. The fixed effect meta-analysis was used to estimate the overall odds ratio (OR) and pooled prevalence was estimated using a random-effects model with a 95% confidence interval (CI).

Results
Totally, 131 articles (91 case–control and 40 cross-sectional studies) met the eligibility criteria. Patients with mental disorders were significantly at higher risk for protozoan parasites than healthy controls (OR: 2.059, 1.830–2.317). The highest pooled OR (2.485, 1.413–4.368) was related to patients with neurodevelopmental disorders, and the highest pooled prevalence was detected in patients with neurodevelopmental disorders (0.341, 0.244–0.446), followed by bipolar and related disorders (0.321, 0.000–0.995). Toxoplasma gondii was the most prevalent protozoan parasite (0.343, 0.228–0.467) in cross-sectional studies and the highest pooled OR was related to Cyclospora cayetanensis (4.719, 1.352–16.474) followed by Cryptosporidium parvum (4.618, 2.877–7.412).

Conclusion
Our findings demonstrated that individuals afflicted with mental disorders are significantly more susceptible to acquiring protozoan parasites in comparison to healthy individuals. Preventive interventions, regular screening, and treatment approaches for parasitic diseases should be considered for patients with mental disorders.
Prolonged hiccups induced by renal infarction: a case report
Authors: Kato Akira , Sato Nobuhiro , Hirose Yasuo , Nomoto Yuji , Ozaki Seiga , Yamaga Saori , Yabe Masahiro 
Published: January 2024

Abstract
Background
Hiccups are common symptoms that last for less than 48 hours. However, we encountered a case of renal infarction in a patient with prolonged hiccup. The relationship between hiccups and renal infarction is important in differentiating patients with prolonged hiccups.

Case presentation
An 87-year-old Japanese man with atrial fibrillation and receiving antithrombotic therapy presented to the emergency department with prolonged hiccups. The patient discontinued antithrombotic therapy for atrial fibrillation due to subcortical bleeding, after which he experienced right back pain. He was diagnosed with right renal infarction based on computed tomography images, and the antithrombotic therapy was continued. The patient’s hiccups ceased, and he was discharged on hospital day 11.

Conclusion
Hiccups can be induced by various clinical conditions. It is hypothesized that the inflammation of the right kidney infarction stimulated the diaphragm and induced prolonged hiccups in this patient; this theory is supported by the computed tomography images. This case report shows that internal organ diseases irritating the diaphragm can cause hiccups, and renal disease should be considered in patients with prolonged hiccups.
Risk factors for bleeding complications in patients undergoing extracorporeal cardiopulmonary resuscitation following out-of-hospital cardiac arrest: a secondary analysis of the SAVE-J II study
Authors: Isokawa Shutaro , Hifumi Toru , Hirano Keita , Watanabe Yu , Horie Katsuhiro , Shin Kijong , Shirasaki Kasumi , Goto Masahiro , Inoue Akihiko , Sakamoto Tetsuya , Kuroda Yasuhiro , Tomita Shiori , Otani Norio , group The SAVE-J II study 
Published: January 2024

Abstract
Background
Bleeding is the most common complication in out-of-hospital cardiac arrest (OHCA) patients receiving extracorporeal cardiopulmonary resuscitation (ECPR). No studies comprehensively described the incidence rate, timing of onset, risk factors, and treatment of bleeding complications in OHCA patients receiving ECPR in a multicenter setting with a large database. This study aimed to analyze the risk factors of bleeding during the first day of admission and to comprehensively describe details of bleeding during hospitalization in patients with OHCA receiving ECPR in the SAVE-J II study database.

Methods
This study was a secondary analysis of the SAVE-J II study, which is a multicenter retrospective registry study from 36 participating institutions in Japan in 2013–2018. Adult OHCA patients who received ECPR were included. The primary outcome was the risk factor of bleeding complications during the first day of admission. The secondary outcomes were the details of bleeding complications and clinical outcomes.

Results
A total of 1,632 patients were included. Among these, 361 patients (22.1%) had bleeding complications during hospital stay, which most commonly occurred in cannulation sites (14.3%), followed by bleeding in the retroperitoneum (2.8%), gastrointestinal tract (2.2%), upper airway (1.2%), and mediastinum (1.1%). These bleeding complications developed within two days of admission, and 21.9% of patients required interventional radiology (IVR) or/and surgical interventions for hemostasis. The survival rate at discharge of the bleeding group was 27.4%, and the rate of favorable neurological outcome at discharge was 14.1%. Multivariable logistic regression analysis showed that the platelet count (< 10 × 104/μL vs > 10 × 104/μL) was significantly associated with bleeding complications during the first day of admission (adjusted odds ratio [OR]: 1.865 [1.252–2.777], p = 0.002).

Conclusions
In a large ECPR registry database in Japan, up to 22.1% of patients experienced bleeding complications requiring blood transfusion, IVR, or surgical intervention for hemostasis. The initial platelet count was a significant risk factor of early bleeding complications. It is necessary to lower the occurrence of bleeding complications from ECPR, and this study provided an additional standard value for future studies to improve its safety.
The role of confidence in the gaze bias effect among economics trainee teachers — results from a digital assessment of economic content knowledge
Authors: Brückner Sebastian , Zlatkin-Troitschanskaia Olga 
Published: January 2024

AbstractIn the present study, we recorded the eye movements of 20 criterion-based selected trainee teachers in economics while they responded to 25 single choice (SC) items in an economic content knowledge (CK) test and rated their confidence for each response in a digital assessment. By using a multilevel modeling approach with crossed random effects, we confirmed prior findings from eye-tracking research on SC tests, which showed longer dwell time on the correct response options (attractor) and shorter dwell time on the distractors are positively linked to correct options. Furthermore, we identified an additional effect on dwell time on the attractor in a moderator model with participants who highly rated their confidence for correct response options. Thus, we identified a specific role of students’ confidence in their CK on the gaze bias effect. We interpret these results in terms of students’ actual understanding of test contents from assessments of their professional knowledge and draw implications for further research and teacher education.
Calculation model and influence factors of thermal conductivity of composite cement-based materials for geothermal well
Authors: Yang Yu , Li Bo , Che Lulu , Li Menghua , Luo Ye , Han Hang 
Published: January 2024

AbstractThe use of cement-based composites (CBC) with high thermal conductivity for geothermal well cementing is extremely important for the efficient development and use of geothermal energy. Accurate prediction of thermal conductivity can save a lot of experimental costs and time. At present, there is no specific calculation model for the thermal conductivity of CBC. In this study, the microstructure, thermal conductivity model and influencing factors of CBC were investigated by experimental tests, theoretical analysis and numerical simulation. The results showed that the cement-based material could be simplified into a two-layer structure of hydrated and unhydrated layers. Mathematical and numerical models based on the coupled Series model and the Maxwell–Eucken model were established to calculate the thermal conductivity for CBC. The mathematical and numerical models were found to be more accurate by comparison with the conventional models and experimental test results. The cubic packing was more favorable than the spherical packing to improve the thermal conductivity of CBC. The plate material had significant anisotropy. The thermal conductivity of CBC showed a rapid decrease followed by a slow decrease, a decrease followed by a slow increase and finally a rapid decrease, a rapid increase followed by an up and down fluctuation and finally a plateau, respectively, with the increase of filler particle diameter, spacing and curing temperature. Based on these results, the effective methods and future research directions were proposed to maximize the thermal conductivity of geothermal well cementing materials in actual engineering applications. The research findings can provide some technical references for the efficient development of geothermal energy and research on CBC with high thermal conductivity.
Scale elasticity and technical efficiency measures in two-stage network production processes: an application to the insurance sector
Authors: Amirteimoori Alireza , Allahviranloo Tofigh , Arabmaldar Aliasghar 
Published: January 2024

AbstractIn performance analysis with tools such as data envelopment analysis, calculations of scale properties of the frontier points are studied using both qualitative and quantitative approaches. When the production process is a bit complicated, the calculation needs to be modified. Most existing studies are focused on a single-stage production process under the constant or variable returns to scale specification. However, some processes have two-stage structures, and, in such processes, the concepts of scale elasticity and returns to scale are inextricably related to the conditions of the stages of production. Thus, an evaluation of efficiency, scale elasticity, and returns to scale is sensitive to stages. In this study, we introduced a procedure to calculate technical efficiency and scale elasticity in a two-stage parallel-series production system. Then, our proposed technical efficiency and scale elasticity programs are applied to real data on 20 insurance companies in Iran. After applying our estimations to a real-world insurance industry, we found that, (i) overall, the total inputs of insurers in the life insurance sector should be reduced by 9%. Moreover, the inputs of nonlife insurers should be reduced by 50%. The final output in the investment sector must be increased by 48%. (ii) There are inefficiencies among all insurers in the investment sector, and to improve technical efficiency, the income from investments should be increased significantly. (iii) Finally, the efficiency and elasticity characterizations of insurers are directly subject to stages.
Stanisław Płaza, Historia prawa w Polsce na tle porównawczym. Cz. 3. Okres międzywojenny, 2001
Authors: Mikołajczyk Marian 
Published: January 2024

Recenzja: Stanisław Płaza, Historia prawa w Polsce na tle porównawczym. Cz. 3. Okres międzywojenny, Księgarnia Akademicka, Kraków 2001, ss. 762.
Kilka lat temu (1997, 1998) ukazały się dwie pierwsze części Historii prawa w Polsce na tle porównawczym autorstwa Stanisława Płazy. Wykład został w ten sposób doprowadzony do początków XX wieku. Oczekiwaliśmy na tom następny, poświęcony okresowi międzywojennemu, choć zdawaliśmy sobie sprawę, że zadanie będzie w tym wypadku o wiele trudniejsze. Pisząc obie poprzednie części Autor mógł przecież w pewnym stopniu wykorzystać swoje wcześniejsze doświadczenia - wszak od początku lat siedemdziesiątych ukazywały się kolejne wersje i wydania podręczników Profesora poświęconych dziejom prawa w Polsce, tak przedrozbiorowej, jak i dziewiętnastowiecznej. Ponadto w przypadku prawa okresu międzywojennego wyraźnie odczuwalny jest brak szczegółowych i monograficznych opracowań, które mogłyby być podstawą dla syntetycznego ujęcia. Wiele istotnych zagadnień pozostaje wciąż poza zasięgiem ustaleń historyków prawa. Co prawda w ostatnich latach da się zauważyć pewien postęp, jednak prace, które ukazały się przed kilku czy też kilkunastu miesiącami nie mogły już zostać wykorzystane przy pisaniu omawianego podręcznika. Autor musiał zatem w wielu wypadkach sam prowadzić szczegółowe badania, studiować międzywojenne piśmiennictwo, sięgać do źródeł normatywnych. Tym większy podziw budzi efekt owych prac potężne, liczące przeszło 760 stron dzieło.
Dziedziczenie beztestamentowe krewnych kognacyjnych w świetle nowel 118 i 127 Justyniana
Authors: Stęplowska Agnieszka 
Published: January 2024

La succession ab intestat des collateraux à la lumière des novelles 118 Et 127de l’empereur Justinien Décréts par l’empereur Justinien, la Novelle 118 de la 543 et aussi la Novelle 127 de l’an 548 qui a complété la loi précédente, réglaient dans la manière complexe la question de la succession ab intestat, qui auparavant resta très complique et incohérente. La succession de ce genre-ci a été basé sur la parentèle avec le défunt et par les deux lois on a égalisé la position des hommes et des femmes en droit de succession. Justinien a divisé les héritiers en quatre classes. La classe plus proche exclua celle plus éloignée. A la première classe appartenaient tous les descendents du défunt sans regarder au grade de leur parentèle. Ils succédaient l’héritage in stirpes. Dans cette classe ont été appelés à la succession surtout les enfants venants du iustum matrimonium. A condition d’être les descendents uniques du defunt, les enfants de celui nées en concubinat ont reçu le droit à la succession spéciale en vertu des réglements séparés (Novelle 89). Selon la Novelle 118, la deuxième classe d’héritiers comprend les ascendents ainsi que les frères germains et les soeurs germaines du défunt. Pourtant les ascendents plus éloignés que propres parents du defunt ne pouvaient hériter de lui qu’à defaut de ses frères et de ses soeurs germaines. La troisième classe a été divisé en deux sous-classes. Dans la première de celles-ci appartenaient les frères et les soeurs du défunt avec leurs enfants. Ce groupe fait éliminer de la succession la seconde sous-classe composée des démi-frères et des démi-soeurs avec leurs enfants. De la succession ont été pourtant exclus les descendants des ceux derniers. Dans la classe IV héritaient d’autres parents latéraux. La reconnaissance conséquente du principe de la parentèle fondée sur les liens de sang s’est manifestée dans la convocation les enfants naturels à l’héritage et dans l’introduction de la succession des ascendents déjà en deuxième classe paralelement avec l’annulation du droit du père à succéder son fils comme un parens manumissor. Grâce à la reconnaissance du principe de cognation ainsi que à l’admission de la possibilité de succéder de biens de son enfant, on a reconnu la pleine capacité juridique de l’enfant de famille. La mise en égalité des droits de deux sexes avait pour la conséquence cela que les soeurs ont acquis les mêmes droits ainsi qu’on a admis la succession les descendents feminins dans les classes I et III. Pareillement s’est ameliorée la position de la mère de famille, laquelle en principe jouissait des mêmes droits de succession que le père.