bluuebunny/crossref_metadata_2025_split huggingface dataset:
Injection of Different Concentrations of Recombinant Humanized Type III Collagen Into the Dermis Promotes Type III and Type I Collagen Deposition as Well as Angiogenesis: An In Vivo Study
Authors: Xiang Haibo , Sun Yimou , Chen Yujie , Wang Guanhuier , Xie Hongbin 
Published: January 2025

BACKGROUND
Type III recombinant humanized collagen (rhCol III) has a promising future in cosmetic applications.

OBJECTIVE
To investigate the histological changes associated with injection of different concentrations of Type III rhCol III into the dermis.

MATERIALS AND METHODS
Twenty mice were divided into a control, low-dose (LD) group, and high-dose (HD) group. The animals were euthanized 1, 2, 3, and 4 weeks after the injection.

RESULTS
Injections of rhCol III induced an increase in collagen formation in a concentration-dependent manner. Type I collagen (Col I) was upregulated in both HD and LD groups to varying extents, often similar to that of Col III. Fibroblast proliferation plays an important role in the deposition of Col I and Col III. Injections of rhCol III promoted angiogenesis at later stages, regardless of concentration dependency.

CONCLUSION
Intradermal injections of rhCol III can promote fibroblast proliferation, enhancing the deposition of both Col I and Col III, and stimulating angiogenesis. Further studies are required to elucidate the mechanisms.
Scleral Lens Use in the Management of Ocular Cicatricial Pemphigoid
Authors: Severinsky Boris , Chambers Shae , Shafi Aysha , Behshad Soroosh , Kim Joung 
Published: January 2025

Purpose:
Symptom management in Ocular Cicatricial Pemphigoid (OCP) is challenging because of the progressive factors of dry eye, scarring, trichiasis, and vision impairment. We set out to evaluate the benefits and therapeutic effects of scleral contact lenses in the management of OCP.

Methods:
A retrospective review was performed over a database of 20 patients (36 eyes) fitted with scleral lenses (SL) at the Department of Ophthalmology, Emory University from May 2018 to April 2021. The fitting indications, time required for ocular surface stabilization, vision rehabilitation success, and ocular complications were evaluated.

Results:
The mean age was 67.4 (range, 43–81) years, with four to one female to male predominance. The mean duration of the follow-up was 16.5 months (range, 1.5–35). The mean wearing time was 9.0±1.9 hr a day, with no overnight wear permitted. All fitted patients reported a subjective improvement in ocular comfort after initiations of SL wear. The mean keratopathy grading improved from 2.1±0.8 to 1.4±0.7 at the last documented visit. The mean visual acuity improved from 0.56±0.51 (logMAR) to 0.34±0.45; in 46% of patient eyes, visual acuity improved by two or more lines. And 96% of fitted eyes responded well to therapy and continued scleral lens wear. Patients who initially presented with fornix shortening, symblepharon, and trichiasis remained stable.

Conclusions:
SL offer ocular surface preservation, symptom relief, and improved visual acuity in patients with ocular pemphigoid. They should be strongly considered as ancillary therapy in patients with OCP.
The Impact of Ramadan Fasting on Dyspeptic Complaints
Authors: Zekey Fethi Sada , Başak Fatih 
Published: January 2025

Objective:
Dyspepsia, a common gastrointestinal issue characterized by upper abdominal discomfort, can be influenced by diet, medication, and lifestyle changes. Ramadan fasting involves refraining from food and drink from dawn to sunset, affecting various physiological processes, including digestion. The fasting period can range from 12 to 18 hours depending on the year, potentially impacting the prevalence of dyspeptic symptoms. This study aimed to assess the effect of fasting on dyspeptic symptoms, taking into account demographic and clinical factors.

Materials and Methods:
A cohort study examined 1258 clinic patients, with 150 meeting specific inclusion criteria. Dyspepsia was diagnosed using Rome IV criteria, with fasting hours taken into consideration. The relationship between dyspepsia, fasting, Non-Steroidal Anti-Inflammatory Drug use, and demographics was analyzed.

Results:
Dyspepsia was more prevalent in females (32%) compared with males (23%), with a higher proportion of non-fasting women among dyspeptic patients. Older patients (>65 y) had a higher prevalence of dyspepsia (P = 0.026). Fasting individuals had a 2.1 times greater likelihood of experiencing dyspeptic symptoms. Although Non-Steroidal Anti-Inflammatory Drug use was lower in fasting patients, no significant association with dyspepsia was observed (P = 0.139).

Conclusion:
Ramadan fasting increases the risk of dyspeptic symptoms, particularly in women and older adults. Factors such as medication timing and dietary changes during fasting may contribute to this risk. Highlighting the importance of the pre-dawn meal (Suhoor) in managing dyspeptic symptoms is essential. Tailored guidance should be provided to individuals at higher risk during Ramadan.
Multicentric primary ectopic meningiomas involving lung and cranial bone barrier: A rare case report
Authors: Zhang Huiyang , Li Xiang , Jiang Yan 
Published: January 2025

Rationale:
Primary ectopic meningiomas (PEMs) are extremely rare, with limited literature available on the subject. Understanding their clinical and radiological characteristics is crucial for accurate diagnosis and treatment.

Patient concerns:
This study presents a case of a multicentric PEM involving the lungs and cranial bone barrier, aiming to provide new insights into its clinical and diagnostic features.

Diagnoses:
A 46-year-old woman with no prior history of intracranial meningiomas or other tumors was found to have multiple lobulated nodules in the lungs during a routine physical examination. Computed tomography scans revealed well-defined lesions with mild to moderate heterogeneous enhancement. Magnetic resonance imaging showed a lesion at the cranial bone barrier, which presented as a high-signal area on T2-weighted FLAIR images and mild to moderate enhancement on T1-weighted images. The magnetic resonance spectroscopy displayed a broad Lip peak but lacked N-acetylaspartate or creatine peaks. Histopathological and immunohistochemical analyses confirmed the diagnosis of multicentric PEMs.

Interventions:
We performed surgical resection of the lesion on the cranial bone plate and conducted follow-up examinations for the multiple lesions in the lungs.

Outcomes:
This case highlights the diagnostic challenges of multicentric PEMs involving the lungs and cranial bone barrier. Due to their extremely low incidence and nonspecific clinical manifestations, a comprehensive evaluation combining radiological, pathological, and immunophenotypic data is essential for accurate diagnosis.

Lessons:
This case underscores the importance of a thorough, multidisciplinary approach to diagnosis and treatment and provides valuable insights for managing similar rare cases.
Efficacy and mechanism of Qianjinweijing Decoction for asthma: Integrating systematic review with meta-analysis and network pharmacology
Authors: Zhang Limin , Su Jin , Wu Xiaozheng , Chen Yunzhi , Li Wen 
Published: January 2025

Background:
Asthma seriously affects people’s survival and quality of life, causing a huge economic burden on society. Modern clinical use of Qianjinweijing Decoction (QJWJ) for the treatment of asthma has achieved good results. However, there is still a lack of research on its efficacy and mechanism of action. Therefore, the purpose of this study is to evaluate the efficacy of QJWJ in the treatment of asthma by systematic review and meta-analysis, and to explore its potential mechanism by network pharmacology.

Methods:
The meta-analysis was performed to search for studies published before May 2023 in 7 databases, and Revman 5.4 and R language softwares were used for analysis. Network pharmacology was based on open databases and softwares such as Cytoscape, Perl, Autoduck Vina, and R language.

Results:
A total of 14 studies were included, involving 1200 patients. The results of the meta-analysis showed that QJWJ could significantly improve the clinical efficacy of asthma patients compared with routine pharmacotherapy (risk ratio = 1.22, 95% CI [1.16, 1.28], P < .00001), enhance lung function, such as FEV1/FVC (mean difference [MD] = 5.63, 95% CI [1.45, 9.81], P = .008), FEV1% (MD = 5.03, 95% CI [4.32, 5.74], P < .00001), PEF (standardized mean difference = 1.37, 95% CI [1.03, 1.71], P < .00001), and increase traditional Chinese medicine syndrome score (MD = −2.50, 95% CI [−4.81, −0.19], P = .03). The results of network pharmacology suggested that the 4 traditional Chinese medicines in QJWJ included 35 active ingredients and 34 potential targets for the treatment of asthma. The core ingredients involved were stigmasterol, β-sitosterol, hederagenin, and gibberellin 7. The core targets were PTGS2, BCL2, and CASP3. The interaction pathway between QJWJ and asthma was mainly enriched in p53, cyclic guanosine monophosphate–protein kinase G, IL-17, and advanced glycation end products-receptor for advanced glycation end products signaling pathways. Molecular docking showed that the core ingredients had good binding activity with the core targets.

Conclusion:
QJWJ is effective in the treatment of asthma, and the therapeutic mechanism may be related to its regulation of inflammation, immunity, and apoptosis.
Beating the odds: Rare prolonged survival of truncus arteriosus: A case report with literature review
Authors: Amaewhule Obuoma Umejuru , Otaigbe Barbara Edewele , Tabansi Petronila Nnenna , Uwadiale Rosemary Atsosime , Emeruwa Victoria Ezinne , Daniel Faithful Miebaka 
Published: January 2025

Rationale:
Truncus arteriosus is a cyanotic congenital heart disease in which the great vessels of the heart fail to separate in utero. Consequently, a single truncal vessel arises from the heart to supply the systemic, coronary, and pulmonary circulations. This lesion causes the total mixing of oxygenated and deoxygenated blood, with an early onset of pulmonary vascular disease. Patients rarely survive beyond the first year of life. This case report highlights a rare survival without intervention up to the ninth year of life.

Patient concerns:
We present the case of a 9-year-old male child with a history of dark discoloration of the lips and digits, easy fatigability, and fast breathing since birth. The patient is currently small for his age, with a bulging anterior chest wall, displaced apex beat, and cardiac murmur. Oxygen saturation was 57% in room air. A chest radiograph showed peri-hilar lymphadenopathy with prominent pulmonary trunks. Pack cell volume was 62%, and the gene expert test was negative.

Diagnoses:
A diagnosis of cyanotic congenital heart disease, truncus arteriosus type III with pulmonary hypertension, was made after diagnostic evaluation with echocardiography.

Interventions:
To manage congestive heart failure, the patient was put on diuretics, including furosemide and spironolactone. Parents could not carry out a definitive surgical repair due to financial constraints.

Outcomes:
Although the patient was able to survive the past year, the child had developed cardiomegaly, signs of pulmonary congestion, decreased oxygenation, and a compensatory increase in red blood cell volume.

Lessons:
Truncus arteriosus is a critical congenital heart defect that has a high potential for morbidity and mortality within the first year of life. It requires immediate intervention and surgical repair in the immediate neonatal period. In rare cases, patients have been able to survive beyond the first year, especially if they also developed pulmonary stenosis. However, this patient had been a survivor for 9 years without another structural anomaly. Given the unique presentation and its rarity, further research is needed into compensatory mechanisms and possible low-cost and accessible alternatives in resource-constrained settings. It also demonstrates that pharmacologic therapy alone is insufficient to prevent mortality.
The efficacy of auricular acupressure combined with emotional freedom techniques on the postoperative pain and anxiety state of patients with lower limb fractures: A randomized clinical controlled trial
Authors: Zhou Xianglong , Zhang Guoqin , Chen Dandan , Yao Huiping , Wang Qi 
Published: January 2025

Background:
Lower limb fractures cause local pain, swelling, ecchymosis, and dysfunction. Clinically, incision and reduction surgery are often employed to treat lower limb fractures. However, postoperative pain can be intense, leading to adverse emotions that hinder subsequent recovery.

Methods:
A total of 99 postoperative patients with lower limb fractures were randomly divided into 3 groups: the control group (routine care, 33 patients), the auricular acupressure group (routine care plus auricular acupressure, 33 patients), and the combined group (routine care, auricular acupressure, and emotional freedom techniques [EFT], 33 patients). Pain levels were measured using the visual analog scale (VAS), and anxiety levels were measured using the self-rating anxiety scale (SAS) at various time points post-intervention. Patient satisfaction with nursing care was also evaluated at the end of the intervention.

Results:
The VAS scores in the combined group were significantly lower than those in the auricular acupressure and control groups at 4, 12, 24, and 48 hours post-intervention (P < .05). Additionally, the SAS scores in the combined group were significantly lower than those in the auricular acupressure and control groups at 72 hours post-intervention (P < .05). The combined group also reported significantly higher satisfaction with nursing care compared to the other 2 groups at the end of the intervention (P < .05).

Conclusion:
Auricular acupressure combined with EFT effectively reduces pain levels, alleviates anxiety, and increases patient satisfaction with nursing care in postoperative patients with lower limb fractures.
Peritoneal dialysis-associated peritonitis caused by Coxiella Burnetii: A case report
Authors: Zhong Yue , Cai Ze-Yu , Wang Jun-Rui , Qi Qi-Ge , Hao Jian 
Published: January 2025

Rationale:
Peritoneal dialysis (PD)-associated peritonitis (PDAP) is the leading cause of PD failure and discontinuation of PD. Several zoonotic pathogens could lead to the development of PDAP. Coxiella burnetii (C. burnetii) was a zoonotic pathogen and the cause of Q fever. However, reports of PDAP caused by C. burnetii are rare. We herein report the first case of PDAP caused by C. burnetii in mainland China.

Patients concerns:
A 45-year-old woman was admitted to our hospital with chief complaint of yellow and cloudy PD effluent for 2 days. She had undergone PD for 5 years due to end-stage renal disease. She was engaged in cattle and sheep breeding. The culture of PD effluent was negative, even for specific species, such as Mycobacteria and fungi.

Diagnoses:
The culture from the PD effluent tested positive for C. burnetii by adopting metagenomic next-generation sequencing on day 37. We diagnosed her as PDAP caused by C. burnetii.

Interventions:
Empirical treatment with multiple broad-spectrum antibiotics (including vancomycin, etimicin, piperacillin) was initially adopted. After identifying C. burnetii as the culprint as the PDAP, the regimen was changed to doxycycline (100 mg twice daily) and moxifloxacin (400 mg once daily) orally, leading to clinical improvement.

Outcomes:
The white blood cell count of the PD effluent decreased to within the normal range and the culture of PD effluent was negative for C. burnetii at the visit of 4 months after discharge. Also, there was no sign for recurrence.

Lessons:
Vigilance should be heightened for PDAP cases with negative culture of PD fluid and poor response to standard broad-spectrum antibiotic treatment, along with a history of cattle and sheep breeding. In such conditions, PD effluent should be tested to detect possible peritonitis caused by C. burnetii, even in patients without symptoms of fever. Prompt pathogen identification and appropriate treatment are crucial for clinical improvement of such cases.
Concurrent ANCA-associated vasculitis and IgG4-related disease in a patient with fever of unknown origin and acute kidney injury: A case report
Authors: Lee Soo Jin , Shin Yujin , Cha Seung Ah , Kim Kyoung Min , Kang Kyung Pyo 
Published: January 2025

Rationale:
It is often challenging to differentiate between IgG4-related disease (IgG4-RD) and antineutrophil cytoplasmic antibody-associated vasculitis (AAV) due to their similar clinical presentations. Recently, growing evidence has suggested a strong connection between AAV and IgG4-RD.

Patient concerns:
A 60-year-old woman was transferred to our hospital with fever and kidney dysfunction. Abdominal computed tomography revealed widespread infiltrative lesions in both kidneys.

Diagnoses:
Laboratory tests and subsequent renal biopsy confirmed both antineutrophil cytoplasmic antibody-associated vasculitis and IgG4-related disease.

Interventions:
We initiated plasmapheresis, oral cyclophosphamide, and high-dose glucocorticoids for treatment. Despite this, the patient’s condition worsened, requiring emergency hemodialysis.

Outcomes:
After 3 months of continued immunosuppressive treatment, renal function improved and hemodialysis was discontinued.

Lessons:
Our case showed an overlap of AAV and IgG4‐RD, which might support the hypothesis of an overlap syndrome of AAV and IgG4-RD. Clinicians should have a high index of suspicion when diagnosing fever of unknown origin, with the possibility of overlapping AAV and IgG4-RD.
Patient-Centered Physical Activity Intervention in Lung Cancer Patients
Authors: Heredia Ciuró Alejandro , Martín Núñez Javier , Navas Otero Alba , Calvache Mateo Andrés , Torres Sánchez Irene , Granados Santiago María , Valenza Marie Carmen 
Published: January 2025

Background
Increasing physical activity levels is a significant unmet need in cancer survivors, and it can likely be enhanced through a better understanding of the interventions developed. Some studies on patient-centered physical activity interventions have shown promising results in increasing daily activity levels among lung cancer survivors. However, the programs present a high heterogeneity, and there is no consensus on the parameters and their effectiveness.

Objective
To examine the effectiveness of patient-centered physical activity interventions on clinical severity and functional capacity in lung cancer patients.

Methods
A systematic review was performed on randomized controlled trials. A literature search was conducted using MEDLINE, Web of Science, Science Direct, and Cochrane Library (last search November 2023). The Cochrane tool and the Grading of Recommendations Assessment, Development, and Evaluation system were used for quality assessment. Pooled data were meta-analyzed for physical activity levels, functional capacity, and cancer-related symptoms.

Results
Fourteen studies, encompassing 1123 lung cancer patients, were included. The treatment status of patients varied. The components of the physical activity programs showed heterogeneity. Results revealed significant differences favoring patient-centered physical activity interventions over the control group for physical activity levels (P < .05), functional capacity (P < .001), and cancer-related symptoms (P < .05).

Conclusion
The results indicate that patient-centered physical activity programs positively enhance physical activity levels, improve functional capacity, and reduce cancer-related symptoms in patients with lung cancer.

Implications for Practice
Patient-centered physical activity interventions show promise in improving the care and management of lung cancer patients. These interventions provide a basis for encouraging lung cancer patients to actively participate in their treatment.
Reduction of Streak Artifacts in the Superior Vena Cava for Better Visualization of Mediastinal Structures Through Virtual Monoenergetic Reconstructions Using a Photon-counting Detector Computed Tomography
Authors: Thater Greta , Frerichs Isabel , Büttner Sylvia , Schoenberg Stefan O. , Froelich Matthias , Ayx Isabelle 
Published: January 2025

Purpose:
Computed tomography (CT) is crucial in oncologic imaging for precise diagnosis and staging. Beam-hardening artifacts from contrast media in the superior vena cava can degrade image quality and obscure adjacent structures, complicating lymph node assessment. This study examines the use of virtual monoenergetic reconstruction with photon-counting detector CT (photon-counting CT) to mitigate these artifacts.

Materials and Methods:
The retrospective study included 50 patients who underwent thoracoabdominal scans. Virtual monoenergetic reconstructions at nine keV levels (60 to 140 keV) were analyzed for Hounsfield Unit (HU) stability, image noise, and artifact index in various regions of interest (ROIs): mediastinal adipose tissue (ROI 1 to 3) and vascular stations (ROI 4 to 6) were compared with reference tissue (ROI 7 to 8). The diagnostic image quality of the keV levels was assessed using a 5-point Likert Scale.

Results:
Lower keV values (60 to 80) exhibited higher image noise and lower HU stability in mediastinal adipose tissue compared with higher energies, with optimal noise reduction observed at 130 keV (ROI 1 to 3). HU stability in vascular structures (ROI 4 to 6) significantly improved above 80 keV, with the best performance at 140 keV. Artifact levels decreased progressively from 60 to 140 keV. Visually, keV levels of 110 keV (96% Likert ≥4) and 120 keV (60% Likert 4) were rated most diagnostically valuable, consistent with technical findings.

Conclusion:
Virtual monoenergetic reconstructions with photon-counting CT effectively reduce beam-hardening artifacts near the superior vena cava, enhancing the visualization of lymph nodes and adjacent structures. This technology advances oncologic imaging by improving diagnostic accuracy in areas previously affected by artifact-related image degradation.
Flavobacterium arundinis sp. nov., Flavobacterium calami sp. nov., Flavobacterium helocola sp. nov. and Flavobacterium flavipallidum sp. nov., isolated from stagnant water in a clump of Phragmites japonica Steud
Authors: Park Sunho , Kim Inhyup , Woo Haejin , Lee Hyunji , Yook Subin , Seo Taegun 
Published: January 2025

Four novel bacterial strains were isolated from stagnant water in a clump of Phragmites japonica Steud. The four Gram-negative, facultatively anaerobic, rod-shaped, non-motile and yellow-coloured strains were designated as DGU11T, DGU38T, DGU41T and DGU99T. The four novel strains exhibited 16S rRNA gene similarities with their closest type strains, ranging from 95.7 to 98.7%, average nucleotide identity values from 75.3 to 95.6% and digital DNA–DNA hybridization values between 17.9 and 59.6%. The cells of strain DGU11T were non-motile and grew at 10−30°C (optimum 25 °C), a pH range of 4.0–12.0 (optimum 9.0) and in the presence of 0–1.0% sodium chloride (NaCl) (optimum 0%). The cells of strain DGU38T were non-motile and grew at 10−35°C (optimum 30 °C), a pH range of 4.0–8.0 (optimum 7.0) and at 0 –2.0% NaCl (optimum 0%). The cells of strain DGU41T were non-motile and grew at 15−50°C (optimum 30 °C), a pH range of 6.0–9.0 (optimum 7.0) and in the presence of 0% NaCl (optimum 0%). The cells of strain DGU99T were non-motile and grew at 15−35°C (optimum 25 °C), a pH range of 6.0–12.0 (optimum 9.0) and in the presence of 0 –2.0% NaCl (optimum 0%). The major fatty acid composition of the four novel strains was iso-C15:0 and C16:1
ω7c/C16:1
ω6c, which is similar to other Flavobacterium species. The proposed names of the novel strains are Flavobacterium arundinis sp. nov. (type strain DGU11T=KACC 23722T=TBRC 19004T), Flavobacterium calami sp. nov. (type strain DGU38T=KACC 23723T=TBRC 19005T), Flavobacterium helocola sp. nov. (type strain DGU41T=KACC 23724T=TBRC 19006T) and Flavobacterium flavipallidum sp. nov. (type strain DGU99T=KACC 23725T=TBRC 19007T).