bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infertility in Fabry’s Disease: role of hypoxia and inflammation in determining testicular damage
Authors: Sansone Luigi , Barreca Federica , Belli Manuel , Aventaggiato Michele , Russo Andrea , Perrone Giulietta A. , Russo Matteo A. , Tafani Marco , Frustaci Andrea 
Published: February 2024

IntroductionFabry’s disease (FD) is a genetic X-linked systemic and progressive rare disease characterized by the accumulation of globotriaosylceramide (GB3) into the lysosomes of many tissues. FD is due to loss-of-function mutations of α-galactosidase, a key-enzyme for lysosomal catabolism of glycosphingolipids, which accumulate as glycolipid bodies (GB). In homozygous males the progressive deposition of GB3 into the cells leads to clinical symptoms in CNS, skin, kidney, etc. In testis GB accumulation causes infertility and alterations of spermatogenesis. However, the precise damaging mechanism is still unknown. Our hypothesis is that GB accumulation reduces blood vessel lumen and increases the distance of vessels from both stromal cells and seminiferous parenchyma; this, in turn, impairs oxygen and nutrients diffusion leading to subcellular degradation of seminiferous epithelium and sterility.MethodsTo test this hypothesis, we have studied a 42-year-old patient presenting a severe FD and infertility, with reduced number of spermatozoa, but preserved sexual activity. Testicular biopsies were analyzed by optical (OM) and transmission electron microscopy (TEM). Activation and cellular localization of HIF-1α and NFκB was analyzed by immunofluorescence (IF) and RT-PCR on homogeneous tissue fractions after laser capture microdissection (LCMD).ResultsOM and TEM showed that GB were abundant in vessel wall cells and in interstitial cells. By contrast, GB were absent in seminiferous epithelium, Sertoli’s and Leydig’s cells. However, seminiferous tubular epithelium and Sertoli’s cells showed reduced diameter, thickening of basement membrane and tunica propria, and swollen or degenerated spermatogonia. IF showed an accumulation of HIF-1α in stromal cells but not in seminiferous tubules. On the contrary, NFκB fluorescence was evident in tubules, but very low in interstitial cells. Finally, RT-PCR analysis on LCMD fractions showed the expression of pro-inflammatory genes connected to the HIF-1α/NFκB inflammatory-like pathway.ConclusionOur study demonstrates that infertility in FD may be caused by reduced oxygen and nutrients due to GB accumulation in blood vessels cells. Reduced oxygen and nutrients alter HIF-1α/NFκB expression and localization while activating HIF-1α/NFκB driven-inflammation-like response damaging seminiferous tubular epithelium and Sertoli’s cells.
Tumor beta2-microglobulin and HLA-A expression is increased by immunotherapy and can predict response to CIT in association with other biomarkers
Authors: Reis Bernhard , Attig Jan , Dziadek Sebastian , Graefe Nico , Heller Astrid , Rieder Natascha , Gomes Bruno 
Published: February 2024

BackgroundDownregulation of MHC class I expression and/or defects in the antigen presentation pathways are commonly reported in human cancers. Numerous studies previously have explored extensively the molecular mechanisms that underlie HLA-class I and Beta2-Microglobulin (B2M) downregulation. However, the techniques presently available to detect expression of MHC class I proteins lack the robustness, specificity and sensitivity needed for systematic integration and analysis in clinical trials. Furthermore, the dynamics of HLA-class I and B2M expression have not been comprehensively studied as a potential biomarker for immunotherapy.MethodsUsing novel, validated, immunohistochemistry (IHC)-based methods for quantifying B2M and HLA-A in tumor samples from diverse cancer types, we have determined loss of B2M and HLA-A proteins in 336 archived, primary specimens and 329 biopsies from metastatic patients collected during Roche-sponsored Phase 1 clinical trials investigating novel immunotherapy candidates as monotherapy or in combination with CPI.ResultsUp to 56% of cases with B2M or HLA-A loss were noted in the investigated tumor types. The frequency of loss was dependent on indication and stage of disease and revealed heterogeneous expression patterns across patients. B2M and HLA-A loss was increased in metastatic lesions compared to primary tumors, indicating selection of MHC class I low clones in metastatic and refractory tumor cells. High on-treatment B2M expression correlated with successful clinical outcome (RECIST), while high baseline B2M did not. A treatment-induced increase of B2M expression was noted in most of the patients with low B2M levels at baseline. The triple biomarker combination of B2M, CD8 and PDL1 strongly improved response prediction to cancer immunotherapy.ConclusionOur results indicate that B2M and HLA-A loss occurs frequently in tumors and is reversed in most instances following immunotherapy which supports the conclusion that MHC class I loss is not the dominant resistance mechanism to CPI treatment. This investigation reveals a highly dynamic expression of HLA-A and B2M in tumors affected by indication, metastatic status, immunophenotype and immunotherapy treatment. Baseline expression levels of B2M on tumors may be of utility as a constituent of a biomarker panel used for selecting patients for immunotherapy clinical trials.
Dl-3-n-butylphthalide attenuates cerebral ischemia/reperfusion injury in mice through AMPK-mediated mitochondrial fusion
Authors: Zhu Ting , Dong Shanshan , Qin Na , Liu Rujuan , Shi Liuliu , Wan Qi 
Published: February 2024

Introduction: NBP is a compound isolated from celery seeds, which was approved by the National Medical Products Administration in 2002 for clinical treatment of ischemic stroke. However, in brain ischemia/reperfusion (I/R) injury, the related research on mitochondrial dynamics and its mechanism of action of NBP still need to be further studied. The aim of this study was to assess NBP on cerebral pathology in ischemic stroke in vivo, with a specific focus on the molecular mechanisms of how NBP promotes mitochondrial fusion.Methods: Male C57BL/6 mice were utilized in this study and were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R). Pre-ischemia, NBP was administered through intraperitoneal (i.p.) injection for 7 days.Results: Our findings demonstrated that NBP effectively reduced infarct volume, improved neurological dysfunction, enhanced cerebral blood flow, and promoted mitochondrial fusion in mice subjected to MCAO/R. More importantly, the pro-fusion effects of NBP were found to be linked to the activation of AMPK/Mfn1 pathway, and with the activation of neurological function, which was partially eliminated by inhibitors of AMPK.Discussion: Our results revealed that NBP is a novel mitochondrial fusion promoter in protecting against ischemic stroke through the AMPK-mediated Mfn1. These findings contribute to the understanding of novel mechanisms involved in the protection of neurological function following NBP treatment for ischemic stroke.
Facilitators and barriers to behavior change in overweight and obesity management using the COM-B model
Authors: Timkova Vladimira , Minarikova Daniela , Fabryova Lubomira , Buckova Jana , Minarik Peter , Katreniakova Zuzana , Nagyova Iveta 
Published: February 2024

IntroductionIncreasing overweight and obesity rates represent one of the global public health challenges. COM-B is a theoretical model used to identify areas to target to achieve behavior change. It identifies three factors that are needed for any behavior to occur: capability, opportunity, and motivation. We aimed to assess the potential facilitators and barriers to behavior change in weight management using the COM-B.MethodsThe study included 139 people with overweight and obesity (mean age 48.81 ± 14.49 years; 64.5% female; body mass index 32.64 ± 6.51 kg/m2; waist-to-height ratio 0.62 ± 0.10) from primary care settings. All participants completed the Brief Measure of Behavior Change (COM-B), the General Self-Efficacy Scale (GSE), the Rosenberg Self-esteem Scale (RSE), and the Overall Evaluation of Health (OEH). Multiple linear regression was performed to analyse the data.ResultsThe associations between sociodemographic and clinical variables and COM-B domains attenuated or were no longer significant when psychological resources were added to the regression models. Self-efficacy was identified as a stronger facilitator of health behavior change (p &lt; 0.001) when compared to self-esteem (p &lt; 0.05). No associations between automatic motivation and psychological resources were identified, however. Automatic motivation was found to be associated with higher age, being in a relationship, and better health.DiscussionBehavioral interventions for weight management should specifically target different components of COM-B. Self-efficacy and self-esteem may play a significant role in individual capabilities, opportunities, and reflective motivation and should be included in tailored public health interventions. Health programs targeting younger and single people, and people with chronic conditions may help to promote sustainable behavior change.
Follow-up cancer care in Danish general practice: a questionnaire study
Authors: Sætre Lisa Maria Sele , Naamansen Steffi Blach , Balasubramaniam Kirubakaran , Søndergaard Jens , Jarbøl Dorte Ejg 
Published: February 2024

Background
The number of patients who have survived cancer has increased substantially owing to improved cancer treatment. This has reinforced the need for effective strategies for follow-up cancer care in general practice.

Aim
To investigate the organisation of follow-up cancer care in Danish general practice and to analyse GPs’ self-assessment of competences regarding patients who have survived cancer and late effects.

Design & setting
A total of 500 Danish general practices were invited to a web-based survey.

Method
Questions comprised organisation of follow-up cancer care, and GPs’ self-assessment of their competences in follow-up care and evaluation of late effects. Covariates considered included sex, age, seniority, and practice type. Analyses were conducted using descriptive statistics and multivariable logistic regression models.

Results
Some 28% of the GPs reported systematic organisation of follow-up cancer care in their clinic. More than half of the GPs assessed themselves as competent in evaluating mental sequelae, existential considerations, and the impact on comorbidities. In contrast, only 19% and 33% of GPs reported competences in sexual and physical sequelae, respectively. Female GPs were less likely to report competences regarding physical and mental sequelae as well as sexual disturbances, and GPs from partnership practices were more likely to report competence in assessing mental sequelae.

Conclusion
Fewer than one in three general practices have organised systematic follow-up cancer care and GPs assess their competence as low with respect to physical sequelae and sexual challenges. This emphasises the need for more systematic organisation and focus on knowledge of late effects in general practice.
GROWTH AND YIELD OF SUNFLOWER (HELIANTHUS ANNUUS L.) UNDER DIFFERENT ORGANIC (FARM YARD MANURE) AND INORGANIC PHOSPHORUS DOSES
Authors: Kaleri Asif , Soomro Nighat Seema , Banbhan Ghulam Mustafa , Wahocho Safdar Ali , Rehman Shafiq-ur- , Kaleri Naseeruddin , Iqbal Khalid , Samoon Rukhsar , Khokhar Farhan , Bughio Aweesha , Baloch Ghulam Asghar 
Published: February 2024

In this study, we investigate the impact of different rates of farmyard manure application on the growth and yield of sunflower (Helianthus annuus L.). Our objective is to assess how various levels of organic fertilizer influence the development and productivity of this crop. The experiment involved five integrated applications of farmyard manure (T1 = 00 tons of farmyard manure + P (0), T2 = 1 ton of farmyard manure, P (30 P kg ha-1), T3 = 3 tons of farmyard manure + (60 P kg ha-1), T4 = 4 tons of farmyard manure + 90 P kg ha-1, and T5 = 5 tons of farmyard manure + (120 P kg ha-1). The results revealed that T5 = 5 tons of farmyard manure and (120 P kg ha-1). resulted best and produced the maximum plant population (m2) (10.5), plant height (273.7 cm), stem girth (10.9 cm), head diameter (53.8 cm), number of seeds head-1 (2696.9), seed weight head-1 (60.7 g), seed index (32.7 g), and seed yield (2698.7 kg ha-1). Whereas the T1=00-ton Farm Yard Manure treatment produced the least results and recorded plant population (m-2) (6.6), plant height (217.7 cm), stem girth (9.3), head diameter (30.3 cm), number of seeds head-1 (1510.7), seed weight head-1 (26.6 g), seed index (23.0 g), and seed yield (2100.0 kg ha-1). It is therefore recommended that, for obtaining higher growth and seed yields of sunflower, farmyard manure should be applied at 5 tons per hectare, and (120 P kg ha-1) respectively. The results concluded that the growth and yield of sunflowers were significantly affected by the application of farmyard manure as compared to the control (no farmyard manure). The grain yield increased linearly with increasing farmyard manure levels. However, the plot fertilized with 5 tons of farmyard manure produced the maximum grain yield of sunflower.
New anthracnose disease caused by Colletotrichum orchidearum on Cattleya sp. orchid in India
Authors: Dutta Suman , Basu Amitava , Maji Pijush Kanti 
Published: February 2024

Cattleya sp. is high value tropical orchid gaining huge popularity due to its great aesthetic value in West Bengal among the non- conventional agriculturist growers. In August, 2017 to February, 2018 and August, 2018 to February, 2019, new anthracnose disease with initial symptoms characterized by: Blight lesions from margin of the leaves forming greyish to whitish centre surrounded by irregular deep brown colour covering large area were observed on Cattleya sp. of orchid in a nursery at Arambagh, Hooghly District, West Bengal, India (23⁰69’80’’N, 87⁰67’34’’E).Disease incidence and severity were recorded up to 22.67% and during survey in January month Cattleya sp. was found to infest with anthracnose where disease severity was recorded up to 23.00%, respectively. The fungus was isolated from the diseased leaves and tentatively identified as Colletotrichum orchidearum based on cultural and morphological characteristics such as spore and acervulus characters. Partial DNA sequences of the ITS1 and ITS4 of the rRNA gene of Small Sub Unit (SSU) of the representative isolate CATA was found to be 100% similar with that of Colletotrichum orchidearumstrain LZJZ6 (Accession number: MK796541, China) and Colletotrichum orchidearum strain LL29-1-3 (Accession number: OM666546, China) obtained from NCBI database. Phylogenetic analysis using the internal transcribed spacer (ITS) region sequences indicated that the isolate CATA (MT158680) was closely related with Colletotrichum orchidearum strain LZJZ6 (Accession number: MK796541) and Colletotrichum orchidearum strain LL29-1-3 (Accession number: OM666546) and clustered together in as a single group with other deposited isolates of Colletotrichum orchidearum (OM666547 from China, MT114868 from Sri Lanka and MT299587 from Brazil). Isolates CATA was found to be pathogenic on the Cattleya sp. orchid. This is the first report of Colletotrichum orchidearum as the causal organism of Anthracnose disease in Cattleya sp.orchid in India.
Testimonios y evidencias
Authors: Pozzi Escot Denise , Sala i Vila Núria , Villar Rocío , Fuentes Sarita 
Published: February 2024

Una práctica recurrente en la arqueología peruana es comparar las evidencias arqueológicas con informaciones aportadas por los cronistas de la conquista y el temprano siglo XVI, quienes brindan datos significativos sobre el imperio incaico y las sociedades coexistentes, su organización económica, política, social, cultos, ideología, entre otros aspectos. En este artículo proponemos un diálogo analítico y cuestionador entre las informaciones de exploradores, viajeros y otros testimonios del s. XVIII y el registro arqueológico, a fin de comprender los vestigios del santuario de Pachacamac, tal como fueron observados y descritos en ese tiempo.
Presentamos los alcances en base a nuestra última intervención en el Templo Pintado (200 – 1470 d.C.) del santuario de Pachacamac, que revela parcialmente la apariencia del oráculo de Pachacamac durante sus últimos momentos de servicio al culto prehispánico, luego de la incursión de los primeros colonizadores españoles, actividades intensivas de saqueo, un parcial abandono y de desde la mirada de los viajeros del S.XVIII, expedicionarios científicos, cartógrafos e intelectuales peruanos.
PENGARUH KEPEMIMPINAN TRANSFORMASIONAL, PERILAKU KERJA DAN DISIPLIN TERHADAP PRESTASI KERJA PEGAWAI PADA KANTOR DPR RI KOMISI IX DI JAKARTA
Authors: Sari Novita , Mardjuni Sukmawati , Mane A. Arifuddin 
Published: February 2024

Penelitian bertujuan untuk mengetahui dan menganalisis pengaruh kepemimpinan transformasional terhadap prestasi kerja pegawai, untuk mengetahui dan menganalisis pengaruh perilaku terhadap prestasi kerja pegawai, dan untuk mengetahui dan menganalisis pengaruh disiplin terhadap prestasi kerja pegawai. Untuk mencapai tujuan tersebut maka digunakan teknik pengumpulan data melalui penyebaran kuesioner dan teknik analisis data menggunakan analisis regresi berganda. Hasil penelitian menemukan bahwa kepemimpinan transformasional mempunyai pengaruh positif dan signifikan terhadap prestasi kerja pegawai pada Kantor DPR RI Komisi IX. Perilaku kerja mempunyai pengaruh positif dan signifikan terhadap prestasi kerja pegawai pada Kantor DPR RI Komisi IX. Disiplin kerja mempunyai pengaruh positif dan signifikan terhadap prestasi kerja pegawai pada Kantor DPR RI Komisi IX.
This study aimed to identify and analyze the effect of transformational leadership on employee performance, to identify and analyze the influence of work behavior on employee performance, to determine and analyze the influence of discipline on employee performance. To achieve this goal, data collection techniques were used by distributing questionnaires, with data analysis technique using multiple regression analysis. The result of the study found that transformational leadership has a positive and significant influence on employee performance at DPR RI Commission IX. Work behavior has a positive and significant influence on employee performance at DPR RI Commission IX. Work discipline has a positive and significant influence on employee performance at DPR RI Commission IX.
ANALISIS KESESUAIAN KOMPENSASI DAN SEMANGAT KERJA TERHADAP KINERJA PEGAWAI PADA KANTOR KECAMATAN RAPPOCINI KOTA MAKASSAR MELALUI KOMITMEN ORGANISASI
Authors: Citra Nur , Saleh Muhammad Yusuf , Said Miah 
Published: February 2024

Tujuan penelitian untuk menganalisis pengaruh kesesuaian kompensasi semangat kerja dan komitmen organisasi terhadap komitmen organisasi, untuk menganalisis pengaruh kesesuaian kompensasi dan semangat kerja terhadap kinerja pegawai, untuk menganalisis pengaruh semangat kerja terhadap kinerja pegawai, serta untuk menganalisis pengaruh kesesuaian kompensasi dan semangat kerja terhadap kinerja pegawai melalui komitmen organisasi. Analisis yang digunakan dalam penelitian ini adalah path analysis. Temuan ini diperoleh bahwa kesesuaian kompensasi memberikan dampak yang secara nyata dalam meningkatkan komitmen organisasi pada kantor Kecamatan Rappocini di Kota Makassar. Semangat kerja secara nyata dapat meningkatkan komitmen organisasi dalam pelaksanaan pekerjaan selama bekerja pada Kantor Kecamatan Rappocini di Kota Makassar. Secara empiris menemukan bahwa semangat kerja memberikan kontribusi terhadap peningkatan kinerja pegawai. Semangat kerja pegawai dapat meningkatkan kinerja pegawai. Komitmen organisasi secara nyata akan dapat meningkatkan kinerja pegawai dalam pelaksanaan kerja yaitu pada Kantor Kecamatan Rappocini di Kota Makassar. Komitmen organisasi berperan dalam memediasi pengaruh kesesuaian kompensasi terhadap kinerja pegawai pada Kantor Kecamatan Rappocini di Kota Makassar. Komitmen organisasi memiliki peran dalam memediasi pengaruh semangat kerja terhadap kinerja pegawai.
The purpose of this study was to analyze the effect of suitability of employee compensation and organizational commitment, to analyze the effect of suitability of compensation and morale on employee performance, to analyze the effect of morale on employee performance, and to analyze the effect of suitability of compensation and morale on employee performance through organizational commitment. The analysis used in this research was path analysis. This finding showed that the suitability of compensation has a real impact on increasing organizational commitment at the Rappocini Sub-district office in Makassar City. Morale can significantly imrpove the organizational commitment in the implementation of work while working at the Rappocini Sub-district Office in Makassar City. Empirically found that morale contributes to improve employee performance. Organizational commitment will significantly increase employee performance in carrying out work at the Rappocini Sub-district Office in Makassar City. Organizational commitment plays a role in mediating the effect of suitability of compensation on employee performance at the Rappocini Sub-district Office in Makassar City. Organizational commitment has a role in mediating the effect of morale on employee performance.
PENGARUH PERTUMBUHAN EKONOMI DAN PENDAPATAN ASLI DAERAH TERHADAP KEMANDIRIAN KEUANGAN DAERAH MELALUI UMKM DI KABUPATEN KEPULAUAN SELAYAR
Authors: Ashary Ulfirah Mutmainnah , Menne Firman , Abduh Thamrin 
Published: February 2024

Tujuan penelitian ini adalah untuk mengetahui dan menganalisis pengaruh pertumbuhan ekonomi terhadap UMKM, untuk mengetahui pengaruh pendapatan asli daerah terhadap UMKM, untuk mengetahui pengaruh pertumbuhan ekonomi terhadap kemandirian keuangan daerah, untuk mengetahui pengaruh pendapatan asli daerah terhadap kemandirian keuangan daerah, untuk mengetahui pengaruh UMKM terhadap kemandirian keuangan daerah, untuk mengetahui pengaruh pertumbuhan ekonomi terhadap kemandirian keuangan daerah melalui UMKM, untuk mengetahui pengaruh pendapatan asli daerah terhadap kemandirian keuangan daerah melalui UMKM di Kabupaten Kepulauan Selayar. Hasil penelitian menemukan bahwa pertumbuhan ekonomi tidak berpengaruh terhadap UMKM dan kemandirian keuangan daerah, pendapatan asli derah berpengaruh terhadap UMKM dan kemandirian keuangan daerah, UMKM tidak berpengaruh terhadap kemandirian keuangan daerah. Hasil uji sobel test menunjukkan bahwa UMKM tidak dapat memediasi pengaruh pertumbuhan ekonomi dan pendapatan asli daerah terhadap kemandirian keuangan daerah. Hal ini dapat diindikasikan bahwa semakin tinggi pendapatan asli daerah maka tingkat kemandirian keuangan daerah semakin baik. karena itu maka disarankan agar pemerintah daerah meningkatkan pendapatan asli daerah dan pertumbuhan ekonomi dengan mendorong sektor UMKM.
The purpose of this study was to determine and analyze the effect of economic growth on MSMEs, to find out the influence of local native income through MSMEs, to determine the effect of economic growth on regional financial independence, To determine the effect of local original income on regional financial independence, to determine the influence of MSMEs on regional financial independence, to determine the effect of economic growth on regional financial independence through MSMEs, to determine the effect of local native income on regional financial independence through MSMEs in Selayar Island Regency. The results of the study found that economic growth has no affect on MSMEs and regional financial independence, local native income affected MSMEs and regional financial independence, MSMEs did not affect regional financial independence. The results of the sobel test showed that MSMEs cannot mediate the effect of economic growth and local original income on regional financial independence. It can be indicated that the higher the local native income, the better the level of regional financial independence. Therefore, it is recommended that local governments increase local native income and economic growth by encouraging the MSMEs sector.