bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arthroscopic Assessment for Intra-articular Disorders in Residual Ankle Disability after Sprain
Authors: Takao Masato , Uchio Yuji , Naito Kohei , Fukazawa Ikuo , Ochi Mitsuo 
Published: February 2005

Background After ankle sprain, there can be many causes of disability, the origins of which cannot be determined using standard diagnostic tools. Hypothesis Ankle arthroscopy is a useful tool in identifying intra-articular disorders of the talocrural joint in cases of residual ankle disability after sprain. Study Design Cohort study (diagnosis); Level of evidence, 2. Methods The authors gathered the independent diagnostic results of physical examination, standard mortise and lateral radiography, stress radiography of the talocrural joint, and magnetic resonance imaging for 72 patients with residual ankle disability lasting more than 2 months after injury (mean, 7 months after injury). They performed arthroscopic procedures and compared the double-blind results. Results In all cases, the arthroscopic results matched those of other means of diagnosis. In 14 cases, the arthroscopic approach exceeded the capabilities of the other methods. Including duplications, 39 patients (54.2%) had anterior talofibular ligament injuries, 17 patients (23.6%) had distal tibiofibular ligament injuries, 29 patients (40.3%) had osteochondral lesions, 13 patients (18%) had symptomatic os subfibulare, 3 patients (4.2%) had anterior impingement exostosis, and 3 patients (4.2%) had impingement due to abnormally fibrous bands. There were only 2 cases in which the cause of symptoms could not be detected by ankle arthroscopy, compared with 16 cases in which the cause of disability could not be detected using standard methods. In 3 cases (17.6%) of distal tibiofibular ligament injuries, 8 cases (27.6%) of osteochondral lesions, and all 3 cases (100%) of impingement of an abnormal fibrous band, ankle arthroscopy was the only method capable of diagnosing the cause of residual ankle pain after a sprain. Conclusion The present results suggest that arthroscopy can be used to diagnose the cause of residual pain after an ankle sprain in most cases that are otherwise undiagnosable by clinical examination and imaging study.
B-cell recovery following rituximab-based therapy is associated with perturbations in stromal derived factor-1 and granulocyte homeostasis
Authors: Dunleavy Kieron , Hakim Frances , Kim Hyun Kyung , Janik John E. , Grant Nicole , Nakayama Takayuki , White Therese , Wright George , Kwak Larry , Gress Ronald , Tosato Giovanna , Wilson Wyndham H. 
Published: February 2005

AbstractThe occurrence of delayed neutropenia following rituximab is poorly defined and of unknown cause. We hypothesized it may be related to perturbations of stromal derived factor-1 (SDF-1) and granulocyte homeostasis. Late-onset neutropenia (LON) was investigated in 130 patients with untreated aggressive B-cell lymphoma receiving DA-EPOCH (dose-adjusted etoposide, prednisone, Oncovin [vincristine], cyclophosphamide, and hydroxydaunorubicin) chemotherapy with or without rituximab. All patients were in remission and had no known causes for neutropenia. LON occurred in 6 (8%) of 76 patients receiving rituximab and 0 of 54 patients not receiving rituximab (P = .04). The median onset was 175 days (range, 77-204 days) after treatment with a median duration of 14 days (range, 11-16 days). In a subset of 24 patients, a significant correlation was found between rapid B-cell recovery and granulocyte decline over the 6-month recovery period (R = –0.53; P = .04). Rapid B-cell recovery directly correlated with prerecovery SDF-1 levels (R = 0.65; P = .015) and SDF-1 decline (R = –0.67; P = .013) after recovery. Our results suggest that early B-cell lymphopoiesis is important for B-cell recovery following rituximab, and that perturbation of SDF-1 during B-cell recovery retards neutrophil egress from the bone marrow. These findings illustrate the dual role of SDF-1 in human B-cell and granulocyte homeostasis.
Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development
Authors: Wang Lisheng , Li Li , Menendez Pablo , Cerdan Chantal , Bhatia Mickie 
Published: February 2005

Abstract
To date, hematopoietic development of human embryonic stem cells (hESCs) has been limited to cell lines cultured in the presence of either mouse embryonic fibroblasts (MEFs) or MEF-conditioned media (MEF-CM). Anonymous xenogenic factors from MEFs or MEF-CM complicate studies of hESC self-renewal and also raise concerns for the potential clinical applications of generating primitive hematopoietic cells from hESC lines maintained under these ambiguous conditions. Here, we demonstrate that hESCs can be cultured over 30 passages in defined conditions in the absence of MEFs or MEF-CM using only serum replacement (SR) media and high concentrations of basic fibroblast growth factor (SR-bFGF). Similar to hESCs cultured in MEF-CM, hESCs cultured in SR-bFGF sustained characteristics of undifferentiated hESCs, proliferative potential, normal karyotype, in vitro and in vivo 3 germ-layer specification and gave rise to hemogenic-endothelial precursors required for subsequent primitive hematopoietic development. Our report demonstrates that anonymous factors produced by feeder cells are not necessary for hESC maintenance and subsequent hematopoietic specification, thereby providing a defined system for studies of hESC self-renewal and hESC-derived hematopoiesis. (Blood. 2005;105:4598-4603)
Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1α in the circulation
Authors: Davis David A. , Singer Kathleen E. , De La Luz Sierra Maria , Narazaki Masashi , Yang Fuquan , Fales Henry M. , Yarchoan Robert , Tosato Giovanna 
Published: February 2005

AbstractThe chemokine stromal-derived factor-1α (SDF-1α) is an essential regulator of hematopoiesis, lymphocyte homing, pre-B-cell growth, and angiogenesis. As SDF-1α is constitutively expressed in many tissues, chemokine function is mostly regulated by proteolytic degradation. Human serum cleaves the 68-amino acid chemokine, SDF-1α, at both termini. The enzyme or enzymes responsible for the removal of the carboxy-terminal lysine from SDF-1α, leading to significant reduction in biologic activity, have not been identified. Using a new biochemical assay for measuring the carboxy-terminal cleavage activity, we purified from serum and plasma a peptidase that specifically removes the carboxy-terminal lysine from SDF-1α and identified it as carboxypeptidase N (CPN, also known as kininase I, arginine carboxypeptidase, and anaphylotoxin inactivator). We demonstrate that SDF-1α in serum and plasma lacks the carboxy terminal lysine, and depletion of CPN from serum and plasma significantly reduces the SDF-1α carboxypeptidase activity. Purified CPN effectively and specifically removes the carboxy-terminal lysine from SDF-1α and significantly reduces the chemokine's biologic activity as a pre-B-cell growth factor and chemoattractant. Thus, in addition to its role as a regulator of the biologic activity of kinins and anaphylatoxins, CPN is an important regulator of the biologic activity of SDF-1α by reducing the chemokine-specific activity. (Blood. 2005;105:4561-4568)
Weekly Paclitaxel and Gemcitabine in Advanced Transitional-Cell Carcinoma of the Urothelium: A Phase II Hoosier Oncology Group Study
Authors: Li Jinxing , Juliar Beth , Yiannoutsos Constantin , Ansari Rafat , Fox Edward , Fisch Michael J. , Einhorn Lawrence H. , Sweeney Christopher J. 
Published: February 2005

Purpose To evaluate the efficacy and toxicity of weekly paclitaxel and gemcitabine in patients with advanced transitional-cell carcinoma (TCC) of the urothelial tract. Patients and Methods Patients with advanced unresectable TCC were enrolled onto this multicenter, community-based, phase II trial. Initially, patients were treated with paclitaxel 110 mg/m2 and gemcitabine 1,000 mg/m2 by intravenous infusion on days 1, 8, and 15 every 28 days. Patients who had an objective response or stable disease continued treatment for a maximum of six courses. Paclitaxel was decreased to 90 mg/m2 and gemcitabine was decreased to 800 mg/m2 for the last 12 patients because of a concerning incidence of pulmonary toxicity in the first 24 patients. Results Thirty-six patients were enrolled between September 1998 and March 2003. Twenty-four patients received the higher doses of paclitaxel and gemcitabine, and 12 patients received the lower doses. Twenty-five (69.4%) of 36 patients had major responses to treatment, including 15 patients (41.7%) with complete responses. With a median follow-up time of 38.7 months, the median survival time was 15.8 months. Grade 3 and 4 toxicities included granulocytopenia (36.1%), thrombocytopenia (8.3%), and neuropathy (16.7%). Five patients (13.9%) had grades 3 to 5 pulmonary toxicity, and one patient had grade 2 pulmonary toxicity. Conclusion Weekly paclitaxel and gemcitabine is an active regimen in the treatment of patients with advanced TCC. However, because of the high incidence of pulmonary toxicity associated with this schedule of paclitaxel and gemcitabine, we recommend against the use of this regimen in this patient population.
Pheochromocytoma Catecholamine Phenotypes and Prediction of Tumor Size and Location by Use of Plasma Free Metanephrines
Authors: Eisenhofer Graeme , Lenders Jacques WM , Goldstein David S , Mannelli Massimo , Csako Gyorgy , Walther McClellan M , Brouwers Frederieke M , Pacak Karel 
Published: February 2005

Abstract
Background: Measurements of plasma free metanephrines (normetanephrine and metanephrine) provide a useful test for diagnosis of pheochromocytoma and may provide other information about the nature of these tumors.
Methods: We examined relationships of tumor size, location, and catecholamine content with plasma and urinary metanephrines or catecholamines in 275 patients with pheochromocytoma. We then prospectively examined whether measurements of plasma free metanephrines could predict tumor size and location in an additional 16 patients.
Results: Relative proportions of epinephrine and norepinephrine in tumor tissue were closely matched by relative increases of plasma or urinary metanephrine and normetanephrine, but not by epinephrine and norepinephrine. Tumor diameter showed strong positive relationships with summed plasma concentrations or urinary outputs of metanephrine and normetanephrine (r = 0.81 and 0.77; P &lt;0.001), whereas relationships with plasma or urinary catecholamines were weaker (r = 0.41 and 0.44). All tumors in which increases in plasma metanephrine were &gt;15% of the combined increases of normetanephrine and metanephrine either had adrenal locations or appeared to be recurrences of previously resected adrenal tumors. Measurements of plasma free metanephrines predicted tumor diameter to within a mean of 30% of actual diameter, and high plasma concentrations of free metanephrine relative to normetanephrine accurately predicted adrenal locations.
Conclusions: Measurements of plasma free metanephrines not only provide information about the likely presence or absence of a pheochromocytoma, but when a tumor is present, can also help predict tumor size and location. This additional information may be useful for clinical decision-making during tumor localization procedures.
Präzision und Richtigkeit in der internen Qualitätskontrolle deutscher Laboratorien – Ergebnisse einer Befragung durch die DGKL. Precision and accuracy in internal quality control of German laboratories –a survey performed by DGKL
Authors: Geilenkeuser Wolf-Jochen 
Published: February 2005

Abstract

Abstract In Germany, the new Guidelines of the German Medical Association (RiliBÄK) have regulated since 2002 the quality assurance requirements for quantitative laboratory measurements. For all measurands listed in the corresponding annex 1, the maximum allowable bias (related to a target value) is stated for internal and external quality control. Originally, these limits were derived statistically from the results of external quality assessment schemes. However, after they were set into operation, it turned out that they are not applicable to some measurands for internal quality control purposes.
Therefore, the advisory board of the BÄK initiated a questionnaire survey among the participants in external quality assessment schemes to get information about the precision and the accuracy of internal quality control measurements.
This paper shows the results of this survey which was performed from July to September 2003.
Effects of Simulated Flash Fire and Variations in Skin Model on Manikin Fire Test
Authors: Song G , Barker R , Thompson D , Grimes R 
Published: February 2005

Abstract
An established numerical model of a manikin fire test, which has the capability of predicting heat transfer through thermally protective clothing exposed to an intense heat environment, is described in this paper. The model considers the fire characteristics simulated in a manikin chamber as well as the insulating air layers between protective garments and the skin surface. The numerical model is applied to analyze the effects of simulated flash fire and variations in a skin model on a manikin test. The study demonstrates that the heat flux measured by 122 thermal sensors over the surface of the manikin exhibits a bell-shaped Gaussian distribution for a short duration in calibration burn. A series of flash fire data with different distributions was generated statistically, and the effects on burn predictions were investigated. The results suggest that the fire distribution affects the burn predictions for 4 s of exposure. The effects of initial temperature distribution, thermal properties, as well as involvement of blood perfusion in a skin model on burn predictions are also discussed. The model predictions demonstrate that the initial temperature distribution in a skin model has a large effect on burn predictions for a one-layer garment exposed to short duration flash fire conditions.
Singers of the landscape: Song, History, and Property Rights in the Malaysian Rain Forest
Authors: Roseman Marina 
Published: February 2005

In the 1930s, British field ethnographer and museum curator H. D. Noone published a map of the Perak/Kelantan watershed, homeland to a number of Orang Asli peoples, including Temiars. With this map their last refuge entered the colonial record. But long before this, the region had been mapped in song by Temiar hunter‐gatherers and horticulturalists. The Temiar inscribe crucial forms of knowledge in song: medical, personal, social, historical, geographic.But their carefully cultivated knowledge has been dismissed. This article recuperates the song map as an ethnohistorical document comprising a new way of making claims to land.
Race and the Culture of Anthropology
Authors: Visweswaran Kamala 
Published: February 2005

The number of panels on “multiculturalism” and “cultural studies” at the AAA's annual meetings has increased significantly. Many anthropologists believe that the discipline has been in the vanguard of debates on racism and multiculturalism, that it stands for precisely those issues raised in the “culture wars”: the equal valuation of all cultures. Yet this is not the case.Multiculturalism and cultural studies have emerged as counterdisciplinary formations which radically foreground race and racial identity precisely because anthropology cannot do so.
Eae19, a New Locus on Rat Chromosome 15 Regulating Experimental Autoimmune Encephalomyelitis
Authors: Sheng Jian Rong , Jagodic Maja , Dahlman Ingrid , Becanovic Kristina , Nohra Rita , Marta Monica , Iacobaeus Ellen , Olsson Tomas , Wallström Erik 
Published: February 2005

AbstractMultiple sclerosis (MS) and its animal model, myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis (MOG-EAE), share a complex genetic predisposition with contributions from the major histocompatibility complex class II genes and many other genes. Linkage mapping in F2 crosses between the susceptible DA rat strain and the resistant ACI or BN rat strains in various models of autoimmune neuroinflammation have repeatedly displayed suggestive linkage to a region on rat chromosome 15. A direct study of this region was undertaken in congenic strains by transferring resistant ACI alleles to the susceptible DA background. Phenotypic analysis demonstrated lower maximal and cumulative EAE scores in the DA.ACI–D15Rat6-D15Rat71 (C15), DA.ACI–D15Rat6-D15Rat48, D15Rat126-D15Rat71 (C15R3b), and DA.ACI–D15Rat23-D15rat71 (C15R4) strains compared to the parental DA rat strain. Linkage analysis was then performed in a (DA × PVG.AV1)F7 advanced intercross line, resulting in a LOD score of 4.7 for the maximal EAE score phenotype at the peak marker D15Rat71 and a confidence interval of 13 Mb, overlapping with the congenic fragment defined by the C15R3b and the C15R4 strains. Thus, a new MOG-EAE locus with the designation Eae19 is identified on rat chromosome 15. There are 32 confirmed or predicted genes in the confidence interval, including immune-responsive gene 1 and neuronal ceroid lipofuscinose gene 5. Definition of loci such as Eae19 enables the characterization of genetically regulated, evolutionary conserved disease pathways in complex neuroinflammatory diseases.
Requirements for Replication Restart Proteins During Constitutive Stable DNA Replication in Escherichia coli K-12
Authors: Sandler Steven J 
Published: February 2005

AbstractConstitutive stable DNA replication (cSDR) is a mechanism for replisome loading in Escherichia coli K-12. This occurs in a dnaA-independent fashion in an rnhA mutant. cSDR is dependent on recA, priA, and transcription. In this report, it is shown that dnaA rnhA mutants using cSDR for initiation of their DNA replication additionally require priB, but not priC, for viability. Two subtle priA missense mutations either eliminated the ability to grow using cSDR (priA301 C479Y) or resulted in very small colonies (priA300 K230R). DnaC809, a priA suppressor, failed to allow priA or priB mutants to grow using cSDR to initiate DNA replication. Furthermore, unlike dnaC+ strains, dnaC809 strains require priC for cSDR. DnaC809,820, a priC-independent suppressor of priA2::kan phenotypes, allowed priA and priC (but not priB) mutants to grow using cSDR to initiate DNA replication. It is also shown that rep and rnhA mutations are synthetically lethal. DnaC809 and dnaC809,820 mutations suppress this lethality. Rep is further shown to be required for cSDR in a dnaC809 strain. A model whereby these different sets of replication restart proteins interact preferentially with substrates associated with either RecA or SSB during replication restart and cSDR, respectively, is proposed.
Mutation Rates, Spectra and Hotspots in Mismatch Repair-Deficient Caenorhabditis elegansSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY863110, AY863111, AY863112, AY863113, AY863114, AY863115, AY863116, AY863117, AY863118, AY863119, AY863120, AY863121, AY863122, AY863123, AY863124, AY863125, AY863126, AY863127, AY863128, AY863129, AY863130, AY863131, AY863132, AY863133, AY863134, AY863135, AY863136, AY863137, AY863138, AY863139, AY863140, AY863141, AY863142, AY863143.
Authors: Denver Dee R , Feinberg Seth , Estes Suzanne , Thomas W Kelley , Lynch Michael 
Published: February 2005

Abstract
Although it is clear that postreplicative DNA mismatch repair (MMR) plays a critical role in maintaining genomic stability in nearly all forms of life surveyed, much remains to be understood about the genome-wide impact of MMR on spontaneous mutation processes and the extent to which MMR-deficient mutation patterns vary among species. We analyzed spontaneous mutation processes across multiple genomic regions using two sets of mismatch repair-deficient (msh-2 and msh-6) Caenorhabditis elegans mutation-accumulation (MA) lines and compared our observations to mutation spectra in a set of wild-type (WT), repair-proficient C. elegans MA lines. Across most sequences surveyed in the MMR-deficient MA lines, mutation rates were ∼100-fold higher than rates in the WT MA lines, although homopolymeric nucleotide-run (HP) loci composed of A:T base pairs mutated at an ∼500-fold greater rate. In contrast to yeast and humans where mutation spectra vary substantially with respect to different specific MMR-deficient genotypes, mutation rates and patterns were overall highly similar between the msh-2 and msh-6 C. elegans MA lines. This, along with the apparent absence of a Saccharomyces cerevisiae MSH3 ortholog in the C. elegans genome, suggests that C. elegans MMR surveillance is carried out by a single Msh-2/Msh-6 heterodimer.
Analysis of Antigens Targeted by Circulating IgG and IgA Antibodies in Patients With Mucous Membrane Pemphigoid Predominantly Affecting the Oral Cavity
Authors: Carrozzo Marco , Cozzani Emanuele , Broccoletti Roberto , Carbone Mario , Pentenero Monica , Arduino Paolo , Parodi Aurora , Gandolfo Sergio 
Published: February 2005

Background: Mucous membrane pemphigoid (MMP) includes a range of variants frequently having oral, particularly gingival, lesions. It has been suggested that MMP exclusively involving the oral cavity could be a distinct subset, but the immunological status of patients with predominantly oral lesions has received little attention.Methods: Indirect immunofluorescence (IIF) on normal human skin and normal human salt‐split skin (SSS) as a substrate and immunoblotting on extracts of human keratinocyte cultures and normal human skin were carried out in 28 consecutive patients with MMP predominantly affecting the gingiva.Results: There was no significant difference between frequency of positive SSS‐IIF among patients with exclusive oral lesions (42%) and with MMP not restricted to the oral cavity (78%) (P = 0.114). By immunoblotting, IgG of 13 sera (46%) reacted with the 180 kD bullous pemphigoid antigen (BPAg2), six (21%) reacted with the 230 kD bullous pemphigoid antigen (BPAg1), and two (7%) reacted with an antigen of 200 kD. IgA of three sera (11%) reacted with BPAg2 and three (11%) reacted with BPAg1. There was no significant difference between frequency of positive immunoblotting among patients with exclusive oral lesions (68%) and with MMP not restricted to the oral cavity (67%) (P = 1.00).Conclusions: Most of the MMP sera contain IgG and IgA antibodies to the two major bullous pemphigoid antigens, independent of clinical presentation. Thus, in the majority of cases, patients with exclusively gingival pemphigoid lesions do not appear to have a distinct variety of mucous membrane pemphigoid. J Periodontol 2004;75:1302‐1308.