bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential effects of microRNAs miR‐21, miR‐99 and miR‐145 on lung regeneration and inflammation during recovery from influenza pneumonia
Authors: Ong Joe Wee Jian , Tan Kai Sen , Lee Joseph Jing Xian , Seet Ju Ee , Choi Hyung Won , Ler Siok Ghee , Gunaratne Jayantha , Narasaraju Teluguakula , Sham Lok‐To , Patzel Volker , Chow Vincent T. 
Published: December 2023

AbstractIn a mouse model of influenza pneumonia, we previously documented that proliferating alveolar type II (AT2) cells are the major stem cells involved in early lung recovery. Profiling of microRNAs revealed significant dysregulation of specific ones, including miR‐21 and miR‐99a. Moreover, miR‐145 is known to exhibit antagonism to miR‐21. This follow‐up study investigated the roles of microRNAs miR‐21, miR‐99a, and miR‐145 in the murine pulmonary regenerative process and inflammation during influenza pneumonia. Inhibition of miR‐21 resulted in severe morbidity, and in significantly decreased proliferating AT2 cells due to impaired transition from innate to adaptive immune responses. Knockdown of miR‐99a culminated in moderate morbidity, with a significant increase in proliferating AT2 cells that may be linked to PTEN downregulation. In contrast, miR‐145 antagonism did not impact morbidity nor the proliferating AT2 cell population, and was associated with downregulation of TNF‐alpha, IL1‐beta, YM1, and LY6G. Hence, a complex interplay exists between expression of specific miRNAs, lung regeneration, and inflammation during recovery from influenza pneumonia. Inhibition of miR‐21 and miR‐99a (but not miR‐145) can lead to deleterious cellular and molecular effects on pulmonary repair and inflammatory processes during influenza pneumonia.
Dynamically adjustable SVEIR(MH) model of multiwave epidemics: Estimating the effects of public health measures against COVID‐19
Authors: Yin Zuo‐jing , Xiao Han , McDonald Stuart , Brusic Vladimir , Qiu Tian‐yi 
Published: December 2023

AbstractThe COVID‐19 pandemic was characterized by multiple subsequent, overlapping outbreaks, as well as extremely rapid changes in viral genomes. The information about local epidemics spread and the epidemic control measures was shared on a daily basis (number of cases and deaths) via centralized repositories. The vaccines were developed within the first year of the pandemic. New modes of monitoring and sharing of epidemic data were implemented using Internet resources. We modified the basic SEIR compartmental model to include public health measures, multiwave scenarios, and the variation of viral infectivity and transmissibility reflected by the basic reproduction number R0 of emerging viral variants. SVEIR(MH) model considers the capacity of the medical system, lockdowns, vaccination, and changes in viral reproduction rate on the epidemic spread. The developed model uses daily infection reports for assessing the epidemic dynamics, and daily changes of mobility data from mobile phone networks to assess the lockdown effectiveness. This model was deployed to six European regions Baden‐Württemberg (Germany), Belgium, Czechia, Lombardy (Italy), Sweden, and Switzerland for the first 2 years of the pandemic. The correlation coefficients between observed and reported infection data showed good concordance for both years of the pandemic (ρ = 0.84–0.94 for the raw data and ρ = 0.91–0.98 for smoothed 7‐day averages). The results show stability across the regions and the different epidemic waves. Optimal control of epidemic waves can be achieved by dynamically adjusting epidemic control measures in real‐time. SVEIR(MH) model can simulate different scenarios and inform adjustments to the public health policies to achieve the target outcomes. Because this model is highly representative of actual epidemic situations, it can be used to assess both the public health and socioeconomic effects of the public health measures within the first 7 days of the outbreak.
Spatial and temporal scaling of extreme rainfall in the United Kingdom
Authors: Wang Zijie , Wilby Robert L. , Yu Dapeng 
Published: December 2023

AbstractExtreme rainfall estimates for ungauged areas contribute to improved resilience to flooding. This study applies a sub‐daily rainfall scaling method to annual daily maximum rainfall series from 102 UK weather stations. We analyse resultant parameters by season, homogenous rainfall region, urban area and geographic factors to investigate how scaling varies temporally and spatially. Dummy regression models are built using these variables to predict the sub‐daily scaling parameter for any location in the United Kingdom. Estimated rainfall intensities are validated with observations and yield Mean Absolute Errors of 3.0, 1.9 and 0.9 mm/h for 1‐, 2‐ and 6‐h events, respectively. We also demonstrate intensity‐duration‐frequency curves at a site in Oxfordshire for scaled and observed data and find that 1‐ to 6‐h, 20‐year rainfall intensities are estimated to be within 9.4%. With such unified scaling relationships, it is possible to derive extreme rainfall for specified durations and return periods at ungauged locations. According to our cross‐validation of estimated and observed intensities, more than 88% of sites fall within 10% error bounds. This method offers a means of generating design rainfall series as input to flood simulation models to evaluate pluvial flood risks in urban areas.
Development of a capillary electrophoresis method based on magnetic solid‐phase extraction for simultaneous and sensitive detection of eight biogenic amines in foods
Authors: Fan Yixuan , Yu Runze , Waterhouse Geoffrey I.N. , Wang Ruiqiang , Sun Yufeng , Xu Zhixiang 
Published: December 2023

AbstractBACKGROUNDBiogenic amines (BAs) in high concentrations are toxic and may cause a series of health symptoms. A sensitive measurement of BA levels is essential for human health. Capillary electrophoresis (CE) has emerged for the separation of eight BAs due to simple sample preparation and highly efficient separation. However, an important drawback for CE is low sensitivity. Magnetic solid‐phase extraction (MSPE) has become a technique of interest owing to its brief operation and low solvent consumption. Hence, MSPE as a pretreatment has great potential to improve CE sensitivity for the analysis of BAs in complex food.RESULTSResults showed that the Pt‐Co‐MWCNTs‐COOH possessed strong magnetism, good reusability, and high adsorptive ability toward eight biogenic amines based on the hydrogen bonding between the −COOH of Pt‐Co‐MWCNTs‐COOH and −NH2 groups of BAs. Using it as an adsorbent, a magnetic solid‐phase extraction coupled with capillary electrophoresis (MSPE‐CE) method was developed to effectively extract and sensitively analyze eight BAs. Under optimal conditions, the MSPE‐CE method has wide linearities (10.0–1000.0 μg L−1) and low limits of detection (1.0–6.1 μg L−1). The accuracy of the developed method yielded recovery values from 82.07% to 102.58%. Meanwhile, the BAs contents in two samples were analyzed using the MSPE‐CE method, with the results consistent with those detected by a high‐performance liquid chromatography method.CONCLUSIONGiven those advantages, the established MSPE‐CE method promises the practical guidance of monitoring a variety of BAs and provides a foundation for the detection of other food hazards. © 2023 Society of Chemical Industry.
Integrated metabolomics and network pharmacology to reveal the lipid‐lowering mechanisms of Qizha Shuangye granules in hyperlipidemic rats
Authors: Zhao Liang , Wang Shuyue , Xu Xiaohang , Guo Wenjun , Yang Jingxuan , Liu Yue , Xie Shengxu , Piao Guangchun , Xu Tunhai , Wang Yang , Xu Yajuan 
Published: December 2023

AbstractBACKGROUNDQizha Shuangye granules (QSG) comprise six traditional Chinese herbal medicines (TCHMs), which have a long history of treating hyperlipidemia (HLP) in China. This study aimed to evaluate the potential lipid‐lowering effects of QSG in an HLP rat model and investigate possible mechanisms. The HLP rat model was induced by a high‐fat diet. Lipid‐related indicators in serum were detected. Serum and liver metabolites were investigated using a liquid chromatography–mass spectrometry‐based metabolomics approach. A herb–compound–target–metabolite (H‐C‐T‐M) network was further constructed to reveal the possible molecular mechanism of QSG to alleviate HLP.RESULTSThe administration of QSG inhibited the HLP‐induced changes in total cholesterol, triglyceride, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, and non‐esterified fatty acid (NEFA) levels. Additionally, QSG significantly attenuated the liver histopathological changes induced by HLP. Metabolomic analysis showed the serum and liver metabolic disorders presented in HLP rats. QSG can reverse the abnormal metabolism caused by HLP. Through network pharmacology analysis, key proteins such as androgen receptor, 3‐hydroxy‐3‐methylglutaryl‐CoA reductase, and peroxisome proliferator‐activated receptor‐α were screened out, and they were speculated to be possible therapeutic targets for QSG to treat HLP.CONCLUSIONThe present study integrated metabolomics and network pharmacology analysis to reveal the efficacy and possible mechanism of QSG in treating HLP, which provides a new reference for the research and development of QSG as a functional food. © 2023 Society of Chemical Industry.
The impact of screening positive for hazardous alcohol use on the diagnostic accuracy of the PTSD Checklist for DSM‐5 among veterans
Authors: Sistad Rebecca E. , Kimerling Rachel , Schnurr Paula P. , Bovin Michelle J. 
Published: December 2023

AbstractThe Posttraumatic Stress Disorder (PTSD) Checklist for DSM‐5 (PCL‐5) is a widely used self‐report measure of PTSD symptoms that has demonstrated strong psychometric properties across settings and samples. Co‐occurring hazardous alcohol use and PTSD are prevalent among veterans, and the effects of alcohol use may impact the performance of the PCL‐5. However, this possibility is untested. In this study, we evaluated the PCL‐5 diagnostic accuracy for veterans who did and did not screen positive for hazardous alcohol use according to the Alcohol Use Disorders Identification Test–Consumption (AUDIT‐C). Participants were 385 veterans recruited from Veterans Affairs primary care clinics. Results indicated that PCL‐5 performance, AUC = .904, 95% CI [.870, .937], did not differ as a product of hazardous alcohol use. PCL‐5 diagnostic utility was comparably high for veterans with, AUC = .904; 95% CI [.846, .962], and without, AUC = .904 95% CI [.861, .946], positive AUDIT‐C screens. Although optimally efficient cutoff scores for veterans who screened positive were higher (i.e., 34–36) than for those with negative screens (i.e., 30), neither were significantly different from the overall PCL‐5 cutoff score (i.e., 32), suggesting that neither veterans with nor without positive AUDIT‐C screens require differential PCL‐5 cutoff scores. The results do underscore the importance of using PCL‐5 cutoff scores in concert with clinical judgment when establishing a provisional PTSD diagnosis and highlight the need for additional study of the impact of comorbidities on PCL‐5 diagnostic accuracy and cutoff scores.
Frontiers in Green Perovskite Light‐Emitting Diodes
Authors: Yu Runnan , Li Changxiao , Zhao Biao , Tan Zhan'ao 
Published: December 2023

AbstractPerovskite, as a star fluorescent material, has the characteristics of adjustable luminescence peak, high color purity, high photoluminescence quantum yield, and low cost, showing significant potential in the photoelectric field. Among the RGB colors for display devices, green is the most sensitive color for human eyes, and green perovskite light‐emitting diodes (GPeLEDs) have aroused much interest in recent years. In this work, the research history of GPeLEDs is reviewed, the perovskites of different dimensions are introduced, and the common synthesis methods of perovskites are discussed. Besides, the strategies to improve the stability and luminous efficiency of GPeLEDs are summarized, including component engineering, phase engineering, ligand engineering, additive engineering, interface engineering, and optical coupling structure strategy. Finally, the development state of GPeLEDs is summarized and prospected. This work is expected to stimulate more unprecedented achievements derived from GPeLEDs, thus promoting their practical applications in future ultra‐high‐definition displays.
Functional Zwitterionic Polyurethanes: State‐of‐the‐Art Review
Authors: Zhang Jinshuai , Lv Siyao , Zhao Xiaoduo , Ma Shuanhong , Zhou Feng 
Published: December 2023

AbstractRecent advancements in bioengineering and medical devices have been greatly influenced and dominated by synthetic polymers, particularly polyurethanes (PUs). PUs offer customizable mechanical properties and long‐term stability, but their inherent hydrophobic nature poses challenges in practically biological application processes, such as interface high friction, strong protein adsorption, and thrombosis. To address these issues, surface modifications of PUs for generating functionally hydrophilic layers have received widespread attention, but the durability of generated surface functionality is poor due to irreversible mechanical wear or biodegradation. As a result, numerous researchers have investigated bulk modification techniques to incorporate zwitterionic polymers or groups onto the main or side chains of PUs, thereby improving their hydrophilicity and biocompatibility. This comprehensive review presents an extensive overview of notable zwitterionic PUs (ZPUs), including those based on phosphorylcholine, sulfobetaine, and carboxybetaine. The review explores their wide range of biomedical applications, from blood‐contacting devices to antibacterial coatings, fouling‐resistant marine coatings, separation membranes, lubricated surfaces, and shape memory and self‐healing materials. Lastly, the review summarizes the challenges and future prospects of ZPUs in biological applications.
Novel markers and networks related to restored skeletal muscle transcriptome after bariatric surgery
Authors: Ouni Meriem , Kovac Leona , Gancheva Sofiya , Jähnert Markus , Zuljan Erika , Gottmann Pascal , Kahl Sabine , de Angelis Martin Hrabĕ , Roden Michael , Schürmann Annette 
Published: December 2023

AbstractObjectiveThe aim of this study was to discover novel markers underlying the improvement of skeletal muscle metabolism after bariatric surgery.MethodsSkeletal muscle transcriptome data of lean people and people with obesity, before and 1 year after bariatric surgery, were subjected to weighted gene co‐expression network analysis (WGCNA) and least absolute shrinkage and selection operator (LASSO) regression. Results of LASSO were confirmed in a replication cohort.ResultsThe expression levels of 440 genes differing between individuals with and without obesity were no longer different 1 year after surgery, indicating restoration. WGCNA clustered 116 genes with normalized expression in one major module, particularly correlating to weight loss and decreased plasma free fatty acids (FFA), 44 of which showed an obesity‐related phenotype upon deletion in mice. Among the genes of the major module, 105 represented prominent markers for reduced FFA concentration, including 55 marker genes for decreased BMI in both the discovery and replication cohorts.ConclusionsPreviously unknown gene networks and marker genes underlined the important role of FFA in restoring muscle gene expression after bariatric surgery and further suggest novel therapeutic targets for obesity.
NADPH oxidase 4 in mouse β cells participates in inflammation on chronic nutrient overload
Authors: Holendová Blanka , Benáková Štěpánka , Křivonosková Monika , Pavluch Vojtěch , Tauber Jan , Gabrielová Eva , Ježek Petr , Plecitá‐Hlavatá Lydie 
Published: December 2023

AbstractObjectiveBy exposing mice carrying a deletion of NADPH oxidase isoform 4, NOX4, specifically in pancreatic β cells (βNOX4−/−) to nutrient excess stimulated by a high‐fat diet (HFD), this study aimed to elucidate the role of β‐cell redox status in the development of meta‐inflammation within the diabetic phenotype.MethodsThe authors performed basic phenotyping of βNOX4−/− mice on HFD involving insulin and glycemic analyses, histochemistry of adipocytes, indirect calorimetry, and cytokine analyses. To characterize local inflammation, the study used caspase‐1 activity assay, interleukin‐1β immunochemistry, and real‐time polymerase chain reaction during coculturing of β cells with macrophages.ResultsThe phenotype of βNOX4−/− mice on HFD was not associated with hyperinsulinemia and hyperglycemia but showed accumulation of excessive lipids in epididymal fat and β cells. Surprisingly, mice showed significantly reduced systemic inflammation. Decreased interleukin‐1β protein levels and downregulated NLRP3‐inflammasome activity were observed on chronic glucose overload in βNOX4−/− isolated islets and NOX4‐silenced INS1‐E cells resulting in attenuated proinflammatory polarization of macrophages/monocytes in vitro and in situ and reduced local islet inflammation.ConclusionsExperimental evidence suggests that NOX4 pro‐oxidant activity in β cells is involved in NLRP3‐inflammasome activation during chronic nutrient overload and participates in local inflammatory signaling and perhaps toward peripheral tissues, contributing to a diabetic inflammatory phenotype.image
Development of biodegradable filaments based on agro‐industrial waste and evaluation of process parameters in final properties
Authors: da Silva Fortes Allef Gabriel , de Abreu Iago Rodrigues , de Sousa Nascimento Junior Renato , de França Railha Antunes , dos Santos Rosa Ana Carolina Ferreira , de Farias Braz Cristiano José , de Carvalho Laura Hecker , Barbosa Renata , Alves Tatianny Soares 
Published: December 2023

AbstractAdditive manufacturing, which has become popular in recent years, is the process of joining materials through the deposition of layers to form parts based on data from a 3D model and fused deposition modeling. The search for biodegradable materials for the production of filaments for 3D printing is increasing and opening up possibilities for the development of new sustainable products, including the use of lignocellulosic waste in the formulation. Therefore, the present work aims to develop polymeric filaments based on polybutylene adipate terephthalate and low content (2.5 and 5 wt%) of peanut and soybean shells and evaluate their potential for 3D printing. The charges were characterized in terms of chemical composition. Tensile and dimensional variation tests were performed on samples manufactured from two different filling orientations: −45/45° and 0/90°. The printability of the filament was based on the manufacture of prototypes of agricultural tubes that were also characterized by thermogravimetry and their morphology through scanning electron and optical microscopy. Both natural residues significantly (p < 0.05) affected the sample dimensions and mechanical properties. However, the filling orientation difference was insignificant (p > 0.05). The filaments produced showed good processability, thermal and dimensional stability. The samples produced showed a satisfactory surface finish without exposing load agglomerates. These results demonstrate the feasibility of using lignocellulosic fillers, such as peanut and soybean shells, in additive manufacturing and indicate the possibility of producing parts with varied geometries.Highlights
Use of lignocellulosic residues in biodegradable filaments.
The filament produced with fillers of peanut and soybean shells showed good processability.
Filament fracture depends on the raster angle.
It was possible to produce tubes with good surface quality and mechanical properties.
Prenatal diagnosis of autosomal recessive renal tubular dysgenesis caused by variants in the ACE gene: Two fetuses with anhydramnios
Authors: Nguyen Thi Sim , Nguyen Duy Anh , Luong Thi Lan Anh , Pho Hong Diep , Thu Huong Nguyen , Huu Bay Luong , Nguyen Duc Anh , Thi Huong Ngo , Thi Canh Thu Than 
Published: December 2023

AbstractIntroductionAutosomal recessive renal tubular dysgenesis (ARRTD) is a rare genetic disorder with a very high mortality rate. The typical symptoms of the disease during pregnancy are oligohydramnios, anhydramnios, and nearly all affected fetuses die after birth or have a stillbirth in late gestation, which can adversely increase maternal risks.MethodsOligohydramnios/anhydramnios can make both amniocentesis for diagnostic testing and morphological evaluation via ultrasound more difficult. In cases of oligohydramnios/anhydramnios suspicious for urinary tract anomalies, amnioinfusion is a meaningful technique that facilitates sampling of amniotic fluid for genetic diagnosis.ResultsWe report two cases of fetuses with anhydramnios and invisible urinary bladder. Clinical exome sequencing from amniotic fluid revealed a biparentally inherited homozygous pathogenic nonsense ACE variant c.2503G 〉 T [p.Glu853Ter] in proband 1 and a biparentally inherited homozygous pathogenic nonsense ACE variant c.2992C 〉 T [p.Gln998Ter] in proband 2. The prognosis was poor and the patients elected to terminate the pregnancies. Additional post‐mortem histopathological examination from the renal tissue of the second fetus showed renal tubular hypoplasia.ConclusionTo our knowledge for the first time, we describe the prenatal diagnosis of ARRTD in Vietnam, and highlight the benefit of detecting ACE variants associated with ARRTD in fetuses with oligohydramnios/anhydramnios through amnioinfusion and amniocentesis, which improves genotype‐phenotype correlations and provides valuable information for reproductive counseling.
Clinical outcomes associated with anti‐Xa‐monitored enoxaparin for venous thromboembolism prophylaxis
Authors: Saunders John A. , Vazquez Sara R. , Hill Joseph A. , Witt Daniel M. 
Published: December 2023

AbstractStudy ObjectiveThe objective of the study was to assess clinical outcomes (composite of any venous thromboembolism [VTE], any bleeding, and mortality) associated with anti‐Xa monitoring in the 30 days following enoxaparin initiation for VTE prophylaxis.DesignRetrospective cohort study.SettingHospital within an academic healthcare system.PatientsPropensity score‐matched hospitalized adults receiving enoxaparin for VTE prophylaxis.InterventionLow‐molecular‐weight heparin anti‐Xa monitoring.Measurements and Main ResultsDuring the 13‐month study period, a total of 6611 patients received enoxaparin for VTE prophylaxis, 301 in the anti‐Xa monitored group and 6310 in the unmonitored group (4.6% received monitoring). The mean age was 52.9 years and 52% of patients were male. The mean body mass index was 31 kg/m2 and the mean creatinine clearance was 109 mL/min. Twenty percent of patients had active cancer. The most common indication for enoxaparin prophylaxis was hospitalization for medical illness (52%) followed by nonorthopedic surgery (37%). The adjusted odds ratio for the primary outcome comparing monitored to unmonitored patients was 1.26 (95% confidence interval, 0.75–2.11). None of the between‐group differences in the individual components of the composite outcome were statistically significant.ConclusionsThirty‐day clinical outcomes in patients receiving enoxaparin for VTE prophylaxis were not improved by anti‐Xa monitoring. Our results support current evidence‐based guideline recommendations against anti‐Xa monitoring for patients receiving enoxaparin for VTE prophylaxis.