bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emotion dysregulation and psychopathological symptoms in non-clinical adolescents: The mediating role of boredom and social media use
Authors: Iannattone Sara , Mezzalira Selene , Bottesi Gioia , Gatta Michela , Miscioscia Marina 
Published: January 2024

Abstract
Background
Emotion dysregulation, boredom, and problematic social media use are well-known vulnerability factors for psychopathology during adolescence; nevertheless, the interplay between these factors remains underinvestigated in the literature. Therefore, the present cross-sectional study aimed to explore the mediating role of boredom and problematic social media use in the relations between emotion dysregulation and both internalizing and externalizing problems in a non-clinical group of Italian adolescents.

Method
721 students (64.6% girls; Mage = 15.49 years ± 1.82) were involved and completed self-report tools assessing emotion dysregulation, boredom, problematic social media use, and psychopathological symptoms. Path analysis was used to test whether boredom and problematic social media use mediated the relation between emotion dysregulation and psychopathology, distinguishing between internalizing and externalizing problems.

Results
Path models showed that emotion dysregulation predicted both internalizing and externalizing problems, as well as boredom and problematic social media use. Importantly, boredom mediated the associations between emotion dysregulation and both psychopathological dimensions, while problematic social media use mediated only the relation with externalizing problems.

Conclusions
Our results highlight that the influence of emotion dysregulation on psychopathology can manifest through different paths, leading to specific symptomatology based on interactions between various variables. In particular, boredom seems to be a transdiagnostic factor for psychopathology in adolescence, whereas problematic social media use would be a dimension-specific factor. The practical implications of these findings are discussed.
Association between maternal polycystic ovarian syndrome undergoing assisted reproductive technology and pregnancy complications and neonatal outcomes: a systematic review and meta-analysis
Authors: Ban Miaomiao , Sun Yifei , Chen Xiaojing , Zhou Xiaoqian , Zhang Yiyuan , Cui Linlin 
Published: January 2024

Abstract
Background
Polycystic ovarian syndrome (PCOS) is recognized as the most prevalent endocrine disorder among women of reproductive age. While the utilization of assisted reproductive technology (ART) has resulted in favorable outcomes for infertility treatment in PCOS patients, the inherent pathophysiological features of the condition give rise to complications and consequences during pregnancy and delivery for both the mother and offspring. This study was to assess the correlation between maternal PCOS and various pregnancy complications and neonatal outcomes undergone ART.

Methods
A systematic search was conducted on PubMed, EmBase, and the Cochrane Library to identify observational studies that investigated the association between PCOS and the risk of various pregnancy complications and neonatal outcomes, including gestational diabetes mellitus (GDM), hypertension in pregnancy (PIH), preeclampsia (PE), preterm birth, abortion, congenital malformations (CA), small for gestational age (SGA), large for gestational age (LGA), low birth weight (LBW), macrosomia, neonatal intensive care unit (NICU) admission and birth weight. Eligible studies were selected based on predetermined inclusion and exclusion criteria. The meta-analysis was conducted using Review Manager and Stata software, with odds ratios (ORs) or mean difference (MD), confidence intervals (CIs), and heterogeneity (I2) being calculated. The search was conducted up to March 2023.

Results
A total of 33 studies with a combined sample size of 92,810 participants were identified. The findings indicate that PCOS is significantly associated with an increased risk of GDM (OR 1.51, 95% CI:1.17–1.94), PIH (OR 1.72, 95% CI:1.25–2.39), PE (OR 2.12, 95% CI:1.49–3.02), preterm birth (OR 1.29, 95% CI:1.21–1.39), and LBW (OR 1.29, 95% CI:1.14–1.47). In subgroup analyses, the risks of GDM (OR 1.80, 95% CI:1.23–2.62) and abortion (OR 1.41, 95% CI:1.08–1.84) were elevated in fresh embryo transferred (ET) subgroup, whereas elevated risk of PE (OR 1.82, 95% CI:1.17–2.83) and preterm birth (OR 1.31, 95% CI:1.21–1.42) was identified in frozen ET subgroup. Whatever with or without hyperandrogenism, patients with PCOS had a higher risk in preterm birth (OR 1.69, 95% CI: 1.31–2.18; OR 1.24, 95% CI:1.02–1.50) and abortion (OR 1.38, 95% CI:1.12–1.71; OR 1.23, 95% CI:1.06–1.43).

Conclusion
Our findings suggest that individuals with PCOS undergone ART are at a notably elevated risk for experiencing pregnancy complications and unfavorable neonatal outcomes. Nevertheless, to establish a definitive association between PCOS and pregnancy-related outcomes, it is necessary to conduct extensive prospective, blinded cohort studies and effectively control for confounding variables.
No evidence for a diminished ovarian reserve among patients with hypertensive disorders of pregnancy: a case control study
Authors: van Bree Bo E. , Jorissen Laura M. , Pattinaja Désirée A.P.M. , Bons Judith A.P. , Spaanderman Marc E.A. , Valkenburg Olivier , van Golde Ron J.T. 
Published: January 2024

Abstract
Background
Existing evidence suggests a relation between cardiovascular dysfunction and diminished ovarian reserve. While it is known that pre-existent cardiovascular dysfunction is also associated with the development of preeclampsia (PE) during pregnancy, we hypothesize that signs of diminished ovarian reserve may occur more frequently among women with a history of hypertensive disorders of pregnancy (HDP). The aim of our study was therefore to analyse if women with a history of HDP show signs of diminished ovarian reserve, represented by lower anti-Mullarian hormone (AMH) levels, compared to controls. For this retrospective observational case control study, patients included women with a history of HDP, whereas controls constituted of women with a history of an uncomplicated pregnancy. The study was conducted in a tertiary referral centre in which all women underwent a one-time cardiovascular and metabolic assessment. Ovarian reserve and markers of cardiovascular function were evaluated, adjusted for age and body mass index (BMI) using linear regression analyses.

Results
163 patients and 81 controls were included over a time span of 3 years. No signs of diminished ovarian reserve i.e. lower AMH level were observed in the patient group versus controls. A subgroup analysis even showed higher AMH levels in late onset HDP as compared to controls (2.8 vs. 2.0 µg/L, p = 0.025). As expected, cardiovascular function markers were significantly less favourable in the patient group compared to controls; higher levels of systolic blood pressure (BP) (5%), diastolic BP (4%), triglycerides (29%), glucose (4%) and insulin levels (81%) (all p < 0.05), whereas high density lipid (HDL) cholesterol was 12% lower (NS).

Conclusions
Despite unfavourable cardiovascular risk profile, the present study does not substantiate the hypothesis that women with HDP show accelerated ovarian ageing as compared to healthy parous controls. Although HDP patients should be warned about their cardiovascular health, they shouldn’t be concerned about unfavourable ovarian reserve status.
Identification and validation of senescence-related genes in polycystic ovary syndrome
Authors: Jiang Danni , Xu Yang , Yang Lin , Li Pengfei , Han Xiaojuan , Li Qianni , Yang Yang , Chao Lan 
Published: January 2024

Abstract
Background
Polycystic ovary syndrome (PCOS) is an exceedingly intractable issue affecting female endocrine and reproductive health. However, the etiology and intricate pathological mechanisms of PCOS remain unclear. Nowadays, aging was found to share multiple common pathological mechanisms with PCOS, which causes probing into the pathogenesis of PCOS from senescence. However, no bioinformatics analyses have specifically focused on connection between PCOS and ovarian aging.

Methods
Differentially expressed aging-related genes in PCOS were identified and then analyzed using function enrichment method. Hub genes were determined based on multiple algorithms, and expression validation of hub genes was performed in both datasets and experiments (human granulosa-like tumor cell line, KGN; human Granulosa Cell, hGCs). Finally, a transcription factor-miRNA-gene network of hub genes was constructed.

Results
Here, we identified 73 aging-related differential expression genes (ARDEGs) by intersecting DEGs in PCOS and senescence-related gene set. Furthermore, we performed biological functions and potential pathways of ARDEGs and potential hub genes were also screened by multiple algorithms. From the perspective of immune dysfunction, we analyzed the correlation between PCOS and immune cells. Finally, TF-miRNA-gene networks were constructed. Finally, TF-miRNA-gene networks were constructed.

Conclusions
Our work aimed to elucidate the relation between PCOS and cellular senescence based on bioinformatics strategy, deepening the understanding of mechanisms and to seek for novel therapy strategies for improving reproductive lifespan and female health. Exploring the potential molecular mechanism of cell aging in PCOS is expected to bring a new breakthrough for PCOS diagnosis and therapy strategies. And this, might deepen our understanding about intricate mechanisms of ovarian aging.
Green synthesis of silver nanoparticles and its environmental sensor ability to some heavy metals
Authors: Ibrahim Nesma H. , Taha Gharib M. , Hagaggi Noura Sh. A. , Moghazy Marwa A. 
Published: January 2024

AbstractThis study marks a pioneering effort in utilizing Vachellia tortilis subsp. raddiana (Savi) Kyal. & Boatwr., (commonly known as acacia raddiana) leaves as both a reducing and stabilizing agent in the green “eco-friendly” synthesis of silver nanoparticles (AgNPs). The research aimed to optimize the AgNPs synthesis process by investigating the influence of pH, temperature, extract volume, and contact time on both the reaction rate and the resulting AgNPs' morphology as well as discuss the potential of AgNPs in detecting some heavy metals. Various characterization methods, such as UV–vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), infrared spectroscopy (IR), Zeta sizer, EDAX, and transmitting electron microscopy (TEM), were used to thoroughly analyze the properties of the synthesized AgNPs. The XRD results verified the successful production of AgNPs with a crystallite size between 20 to 30 nm. SEM and TEM analyses revealed that the AgNPs are primarily spherical and rod-shaped, with sizes ranging from 8 to 41 nm. Significantly, the synthesis rate of AgNPs was notably higher in basic conditions (pH 10) at 70 °C. These results underscore the effectiveness of acacia raddiana as a source for sustainable AgNPs synthesis. The study also examined the AgNPs' ability to detect various heavy metal ions colorimetrically, including Hg2+, Cu2+, Pb2+, and Co2+. UV–Vis spectroscopy proved useful for this purpose. The color of AgNPs shifts from brownish-yellow to pale yellow, colorless, pale red, and reddish yellow when detecting Cu2+, Hg2+, Co2+, and Pb2+ ions, respectively. This change results in an alteration of the AgNPs' absorbance band, vanishing with Hg2+ and shifting from 423 to 352 nm, 438 nm, and 429 nm for Cu2+, Co2+, and Pb2+ ions, respectively. The AgNPs showed high sensitivity, with detection limits of 1.322 × 10–5 M, 1.37 × 10–7 M, 1.63 × 10–5 M, and 1.34 × 10–4 M for Hg2+, Cu2+, Pb2+, and Co2+, respectively. This study highlights the potential of using acacia raddiana for the eco-friendly synthesis of AgNPs and their effectiveness as environmental sensors for heavy metals, showcasing strong capabilities in colorimetric detection.
Minimal aromatic aldehyde reduction (MARE) yeast platform for engineering vanillin production
Authors: Mo Qiwen , Yuan Jifeng 
Published: January 2024

Abstract
Background
Vanillin represents one of the most widely used flavoring agents in the world. However, microbial synthesis of vanillin is hindered by the host native metabolism that could rapidly degrade vanillin to the byproducts.

Results
Here, we report that the industrial workhorse Saccharomyces cerevisiae was engineered by systematic deletion of oxidoreductases to improve the vanillin accumulation. Subsequently, we harnessed the minimal aromatic aldehyde reduction (MARE) yeast platform for de novo synthesis of vanillin from glucose. We investigated multiple coenzyme-A free pathways to improve vanillin production in yeast. The vanillin productivity in yeast was enhanced by multidimensional engineering to optimize the supply of cofactors (NADPH and S-adenosylmethionine) together with metabolic reconfiguration of yeast central metabolism. The final yeast strain with overall 24 genetic modifications produced 365.55 ± 7.42 mg l−1 vanillin in shake-flasks, which represents the best reported vanillin titer from glucose in yeast.

Conclusions
The success of vanillin overproduction in budding yeast showcases the great potential of synthetic biology for the creation of suitable biocatalysts to meet the requirement in industry. Our work lays a foundation for the future implementation of microbial production of aromatic aldehydes in budding yeast.

Graphical Abstract
Combining a prioritization strategy and functional studies nominates 5’UTR variants underlying inherited retinal disease
Authors: Dueñas Rey Alfredo , del Pozo Valero Marta , Bouckaert Manon , Wood Katherine A , Van den Broeck Filip , Daich Varela Malena , Thomas Huw B , Van Heetvelde Mattias , De Bruyne Marieke , Van de Sompele Stijn , Bauwens Miriam , Lenaerts Hanne , Mahieu Quinten , Josifova Dragana ,   Genomics England Research Consortium, Rivolta Carlo , O’Keefe Raymond T , Ellingford Jamie , Webster Andrew R , Arno Gavin , Ayuso Carmen , De Zaeytijd Julie , Leroy Bart P , De Baere Elfride , Coppieters Frauke 
Published: January 2024

Abstract
Background
5’ untranslated regions (5’UTRs) are essential modulators of protein translation. Predicting the impact of 5’UTR variants is challenging and rarely performed in routine diagnostics. Here, we present a combined approach of a comprehensive prioritization strategy and functional assays to evaluate 5’UTR variation in two large cohorts of patients with inherited retinal diseases (IRDs).

Methods
We performed an isoform-level re-analysis of retinal RNA-seq data to identify the protein-coding transcripts of 378 IRD genes with highest expression in retina. We evaluated the coverage of their 5’UTRs by different whole exome sequencing (WES) kits. The selected 5’UTRs were analyzed in whole genome sequencing (WGS) and WES data from IRD sub-cohorts from the 100,000 Genomes Project (n = 2397 WGS) and an in-house database (n = 1682 WES), respectively. Identified variants were annotated for 5’UTR-relevant features and classified into seven categories based on their predicted functional consequence. We developed a variant prioritization strategy by integrating population frequency, specific criteria for each category, and family and phenotypic data. A selection of candidate variants underwent functional validation using diverse approaches.

Results
Isoform-level re-quantification of retinal gene expression revealed 76 IRD genes with a non-canonical retina-enriched isoform, of which 20 display a fully distinct 5’UTR compared to that of their canonical isoform. Depending on the probe design, 3–20% of IRD genes have 5’UTRs fully captured by WES. After analyzing these regions in both cohorts, we prioritized 11 (likely) pathogenic variants in 10 genes (ARL3, MERTK, NDP, NMNAT1, NPHP4, PAX6, PRPF31, PRPF4, RDH12, RD3), of which 7 were novel. Functional analyses further supported the pathogenicity of three variants. Mis-splicing was demonstrated for the PRPF31:c.-9+1G>T variant. The MERTK:c.-125G>A variant, overlapping a transcriptional start site, was shown to significantly reduce both luciferase mRNA levels and activity. The RDH12:c.-123C>T variant was found in cis with the hypomorphic RDH12:c.701G>A (p.Arg234His) variant in 11 patients. This 5’UTR variant, predicted to introduce an upstream open reading frame, was shown to result in reduced RDH12 protein but unaltered mRNA levels.

Conclusions
This study demonstrates the importance of 5’UTR variants implicated in IRDs and provides a systematic approach for 5’UTR annotation and validation that is applicable to other inherited diseases.
Uterine rupture in the first trimester: a case report and review of the literature
Authors: Mutiso Steve Kyende , Oindi Felix Mwembi , Mundia Debbie Muthoni 
Published: January 2024

Abstract
Background
Uterine rupture is a rare complication that can occur in the first trimester of pregnancy. It can lead to serious maternal morbidity or mortality, which is mostly due to catastrophic bleeding. First trimester uterine rupture is rare; hence, diagnosis can be challenging as it may be confused with other causes of early pregnancy bleeding such as an ectopic pregnancy. We present a case of first trimester scar dehiscence and conduct a literature review of this rare condition.

Case presentation
A 39-year-old African patient with four previous hysterotomy scars presented with severe lower abdominal pain at 11 weeks of gestation. She had two previous histories of third trimester uterine rupture in previous pregnancies with subsequent hysterotomies and repair. She underwent a diagnostic laparoscopy that confirmed the diagnosis of a 10 cm anterior wall uterine rupture. A laparotomy and repair of the rupture was subsequently done.

Conclusion
In conclusion, the case presented adds to the body of evidence of uterine scar dehiscence in the first trimester. The risk factors, clinical presentation, diagnostic imaging, and management outlined may help in early identification and management of this rare but life threatening condition.
The association of vasoactive-inotropic score and surgical patients’ outcomes: a systematic review and meta-analysis
Authors: Sun Yan-ting , Wu Wei , Yao Yun-tai 
Published: January 2024

Abstract
Background
The objective of this study is to conduct a systematic review and meta-analysis examining the relationship between the vasoactive-inotropic score (VIS) and patient outcomes in surgical settings.

Methods
Two independent reviewers searched PubMed, Web of Science, EMBASE, Scopus, Cochrane Library, Google Scholar, and CNKI databases from November 2010, when the VIS was first published, to December 2022. Additional studies were identified through hand-searching the reference lists of included studies. Eligible studies were those published in English that evaluated the association between the VIS and short- or long-term patient outcomes in both pediatric and adult surgical patients. Meta-analysis was performed using RevMan Manager version 5.3, and quality assessment followed the Joanna Briggs Institute (JBI) Critical Appraisal Checklists.

Results
A total of 58 studies comprising 29,920 patients were included in the systematic review, 34 of which were eligible for meta-analysis. Early postoperative VIS was found to be associated with prolonged mechanical ventilation (OR 5.20, 95% CI 3.78–7.16), mortality (OR 1.08, 95% CI 1.05–1.12), acute kidney injury (AKI) (OR 1.26, 95% CI 1.13–1.41), poor outcomes (OR 1.02, 95% CI 1.01–1.04), and length of stay (LOS) in the ICU (OR 3.50, 95% CI 2.25–5.44). The optimal cutoff value for the VIS as an outcome predictor varied between studies, ranging from 10 to 30.

Conclusion
Elevated early postoperative VIS is associated with various adverse outcomes, including acute kidney injury (AKI), mechanical ventilation duration, mortality, poor outcomes, and length of stay (LOS) in the ICU. Monitoring the VIS upon return to the Intensive Care Unit (ICU) could assist medical teams in risk stratification, targeted interventions, and parent counseling.

Systematic review registration
PROSPERO CRD42022359100.
A knowledge-graph based text summarization scheme for mobile edge computing
Authors: Yu Zheng , Wu Songyu , Jiang Jielin , Liu Dongqing 
Published: January 2024

AbstractAs the demand for edge services intensifies, text, being the most common type of data, has seen a significant expansion in data volume and an escalation in processing complexity. Furthermore, mobile edge computing (MEC) service systems often faces challenges such as limited computational capabilities and difficulties in data integration, requiring the development and implementation of more efficient and lightweight methodologies for text data processing. To swiftly extract and analysis vital information from MEC text data, an automatic generation scheme of multi-document text summarization based on knowledge graph is proposed in this paper, named KGCPN. For the text data from MEC devices and applications, the natural language processing technology is used to execute the data pre-processing steps, which transforms the MEC text data into a computationally tractable and semantically comprehensible format. Then, the knowledge graph of multi-document text is constructed by integrating the relationship paths and entity descriptions. The nodes and edges of the knowledge graph serve to symbolize the semantic relationships within the text, and the Graph Convolution Neural network (GCN) is used to understand the text and learn the semantic representation. Finally, a pointer-generator network model accepts the encoding information from GCN and automatically generate a general text summarization. The experimental results indicate that our scheme can effectively facilitate the smart pre-processing and integration of MEC data.
Utilizing neurodegenerative markers for the diagnostic evaluation of amyotrophic lateral sclerosis
Authors: Klíčová Kateřina , Mareš Jan , Menšíková Kateřina , Kaiserová Michaela , Friedecký David , Kaňovský Petr , Strnad Miroslav , Matěj Radoslav 
Published: January 2024

Abstract
Background
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by progressive deterioration of upper and lower motor neurons. A definitive diagnostic test or biomarker for ALS is currently unavailable, leading to a diagnostic delay following the onset of initial symptoms. Our study focused on cerebrospinal fluid (CSF) concentrations of clusterin, tau protein, phosphorylated tau protein, and beta-amyloid1–42 in ALS patients and a control group.

Methods
Our study involved 54 ALS patients and 58 control subjects. Among the ALS patients, 14 presented with bulbar-onset ALS, and 40 with limb-onset ALS. We quantified biomarker levels using enzyme-linked immunosorbent assay (ELISA) and compared the results using the Mann–Whitney U-test.

Results
Significant elevations in neurodegenerative markers, including tau protein (p < 0.0001), phosphorylated tau protein (p < 0.0001), and clusterin (p = 0.038), were observed in ALS patients compared to controls. Elevated levels of tau protein and phosphorylated tau protein were also noted in both bulbar and limb-onset ALS patients. However, no significant difference was observed for beta-amyloid1–42. ROC analysis identified tau protein (AUC = 0.767) and p-tau protein (AUC = 0.719) as statistically significant predictors for ALS.

Conclusion
Our study demonstrates that neurodegenerative marker levels indicate an ongoing neurodegenerative process in ALS. Nonetheless, the progression of ALS cannot be predicted solely based on these markers. The discovery of a specific biomarker could potentially complement existing diagnostic criteria for ALS.