bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medullary bundles in Caryophyllales: form, function, and evolution
Authors: Cunha Neto Israel L. , Rossetto Elson Felipe S. , Gerolamo Caian S. , Hernández‐Gutiérrez Rebeca , Sukhorukov Alexander P. , Kushunina Maria , Melo‐de‐Pinna Gladys F. A. , Angyalossy Veronica 
Published: October 2023

Summary

The occurrence of conducting vascular tissue in the pith (CVTP) of tracheophytes is noteworthy. Medullary bundles, one of the remarkable examples of CVTP, evolved multiple times across angiosperms, notably in the Caryophyllales. Yet, information on the occurrence of medullary bundles is fragmented, hampering our understanding of their structure–function relationships, and evolutionary implications.

Using three plastid molecular markers (matK, rbcL, and rps16 intron), a phylogeny is constructed for 561 species of Caryophyllales, and anatomical data are assembled for 856 species across 40 families to investigate the diversity of medullary bundles, their function, evolution, and diversification dynamics. Additionally, correlated evolution between medullary bundles and successive cambia was tested.

Medullary bundles are ancestrally absent in Caryophyllales and evolved in core and noncore families. They are structurally diverse (e.g. number, arrangement, and types of bundles) and functionally active throughout the plant's lifespan, providing increased hydraulic conductivity, especially in herbaceous plants. Acquisition of medullary bundles does not explain diversification rate heterogeneity but is correlated to a higher diversification rate.

Disparate developmental pathways were found leading to rampant convergent evolution of CVTP in Caryophyllales. These findings indicate the diversification of medullary bundles and vascular tissues as another central theme for functional and comparative molecular studies in Caryophyllales.
The efficacy of oral corticoids in treating complex regional pain syndrome: A retrospective cohort study
Authors: van den Berg Corinne , Huygen Frank J. P. M. , Tiemensma Jitske 
Published: October 2023

AbstractObjectivesThere is growing evidence supporting the role of inflammatory mechanisms in complex regional pain syndrome (CRPS). Corticoids, as most effective anti‐inflammatory drugs, are widely used in treating inflammation. The aim of this study was to retrospectively assess the efficacy of oral corticoid treatment in CRPS patients.MethodsPatients treated at the center of pain medicine in the Erasmus University Medical Centre between January 2015 and January 2020 were approached to partake in this study. Medical records were screened for age, gender, medical history, duration of CRPS, and CRPS severity score. Also, treatment effect, dose and duration, pain scores (NRS), and side effects were extracted from medical records. In addition, global perceived effect was completed in patients treated with corticoids.ResultsBetween January 2015 and January 2020, twenty‐nine CRPS patients received corticoids and met the inclusion criteria. One extreme outlier was excluded and treatment effect was unknown for one patient. Average daily dose was 28.9 mg (range 10–30 mg) and the mean treatment duration was 10.5 days (7–21 days). Fourteen patients (51.9%) responded positively to treatment and thirteen (48.1%) did not respond. Side effects were reported in five patients (17.9%).ConclusionsCorticoid treatment was effective in more than half of the patients. With only mild side effects reported the treatment also appears to be relatively safe. Further research is needed to investigate the efficacy of corticoids in treating (early) CRPS, preferably in an intervention study.
Polydopamine‐curcumin coating of titanium for remarkable antibacterial activity via synergistic photodynamic and photothermal properties
Authors: Guo Qing , Li Peiyuan , Zhang Ying , Yan Hongjun , Yan Qiuyan , Su Rixiang , Su Wei 
Published: October 2023

AbstractCombined photothermal therapy (PTT) and photodynamic therapy (PDT) has emerged as a novel and effective antibacterial strategy. In order to endow titanium (Ti) with antibacterial properties, the Ti‐PDA‐Cur composite was prepared using the excellent adhesion properties of polydopamine (PDA) to load curcumin (Cur) on the surface of Ti. The Ti‐PDA‐Cur coating can produce singlet oxygen (1O2) and heat under 405 + 808 nm light irradiation, which can effectively kill Staphylococcus aureus and Escherichia coli. Moreover, the cytotoxicity and hemolysis rate of Ti‐PDA‐Cur were low, indicating its good biocompatibility. Therefore, this study provided a new strategy for the development of new Ti implants.
Kripke's knowledge argument against materialism
Authors: Renero Adriana 
Published: October 2023

AbstractIn his unpublished 1979 Lectures on the Philosophy of Mind, Saul Kripke offers a knowledge argument against materialism focusing on deaf people who lack knowledge of auditory experience. Kripke's argument is a precursor of Frank Jackson's better‐known knowledge argument against materialism (1982). The paper sets out Kripke's argument, brings out its interest and philosophical importance, and explores some similarities and differences between Kripke's knowledge argument and Jackson's.
Case report of an extra‐oral cutaneous sinus tract of endodontic origin in a patient with autism spectrum disorder
Authors: Ruck Patrick , Morris Heather T. , Thikkurissy Sarat 
Published: October 2023

AbstractBackground and OverviewExtraoral cutaneous sinus tracts (EOCST) are often misdiagnosed due to their unexpected appearance without history of odontogenic pain, discomfort, or cellulitis. Diagnosis can be further confounded in those with sensory processing difficulties since patients can have a hypersensitivity to sensory input, but simultaneously demonstrate hyposensitivity and indifference toward pain. EOCST misdiagnosis and resultant mismanagement can lead to multiple surgical excisions, biopsies, and elongated antibiotic regimens, with eventual lesion recurrence.Case DescriptionA 19‐year‐old white male with autism spectrum disorder in the period of transitional dental care presented with a history of a chronic EOCST. The patient required sedation for evaluation, biopsy, and was initially managed by infectious disease under the impression of an actinomycosis infection. The patient completed a twelve‐month course of antibiotic therapy with subsequent lesion re‐occurrence. Eventually, the diagnosis of an EOCST of dental origin was confirmed. It was determined that pulpal necrosis was due to localized dental trauma of the lower left central incisor, as a result of a self‐injurious behavior. Root canal treatment eventually led to the resolution of the lesion. It took three years from initial clinical presentation for the resolution of the lesion.Conclusions and Practical ImplicationsCollaboration between the medical and dental healthcare team in diagnosis and treatment planning for a patient with special needs is essential to ensure prompt and appropriate care for this patient group.
Forecasting stability and growth pact compliance using machine learning
Authors: Baret Kéa , Barbier‐Gauchard Amélie , Papadimitriou Théophilos 
Published: October 2023

AbstractThe 2011 reform of the Stability and Growth Pact (1996) strengthened the European Commission's monitoring of EU member states' public finance. Failure to comply with the 3% limit on public deficit triggers an audit. In this paper, we present a machine learning based forecasting model for compliance with the 3% limit. We use data from 2006 to 2018 (a turbulent period including the Global Financial Crisis and the Sovereign Debt Crisis) for the 28 EU member states. After identifying 8 features as predictors among 138 variables, forecasting is performed using a support vector machine (SVM) algorithm. The proposed model achieved a forecasting accuracy of nearly 92% and outperformed the logit model used as a benchmark.
The international trade in human vaccines before COVID‐19
Authors: Segarra‐Blasco Agustí , Teruel Mercedes , Cattaruzzo Sebastiano 
Published: October 2023

AbstractThe recent COVID‐19 pandemic has highlighted the importance of global access to human vaccines. There is, however, no empirical work on either the unequal distribution of international trade or on its determinants. Applying a gravity model to the UN Comtrade database between 2000 and 2019, we explain the patterns of bilateral trade across 116 countries. Using the Poisson Pseudo‐Maximum Likelihood methodology, our results show that inequalities in international vaccine trade have steadily increased. In general, supply and demand drivers play a role in explaining the flow of human vaccines. The impact of these drivers varies depending on the income level of the importing country. High‐income countries intensify their flows with demand factors such as GDP per capita, or supply factors such as the location of big pharmaceutical companies. Conversely, low‐income countries receive more vaccines according to their population. Our results show that a poor country that houses a big pharmaceutical company acts as an exporting platform to developing and poor countries. Middle‐income countries present features similar to rich countries both by producing more and also by exporting more. The imbalance endangers the global fight against the current COVID‐19 pandemic.
Intrinsic Diophantine approximation on circles and spheres
Authors: Cha Byungchul , Kim Dong Han 
Published: October 2023

AbstractWe study Lagrange spectra arising from intrinsic Diophantine approximation of circles and spheres. More precisely, we consider three circles embedded in or and three spheres embedded in or . We present a unified framework to connect the Lagrange spectra of these six spaces with the spectra of and . Thanks to prior work of Asmus L. Schmidt on the spectra of and , we obtain as a corollary, for each of the six spectra, the smallest accumulation point and the initial discrete part leading up to it completely.
Increased iron utilization and oxidative stress tolerance in a
Vibrio cholerae flrA
mutant confers resistance to amoeba predation
Authors: Hoque M. Mozammel , Noorian Parisa , Espinoza-Vergara Gustavo , Adhikary Srijon , To Joyce , Rice Scott A. , McDougald Diane 
Published: October 2023

ABSTRACT

The flagellar transcriptional regulator
flrA
initiates the regulatory cascade of flagellum synthesis in
Vibrio cholerae
. Previously, we observed an increase in sub-populations of
V. cholerae
carrying mutations in the
flrA
gene during long-term co-adaptation with the amoeba host
Acanthamoeba castellanii
. The
flrA
mutants exhibit increased growth and intracellular survival in the amoeba host but the molecular mechanisms were unknown. Using an in-frame deletion of
flrA,
here we show that the absence of
flrA
increases pathogen growth and induces a distinct
V. cholerae
transcriptomic signature during amoeba predation. Transcriptome analysis of a
flrA
mutant in
A. castellanii
revealed that several genes involved in iron acquisition and amino acid biosynthesis are highly up-regulated compared to the wild-type strain. Furthermore, we show that iron availability is crucial for the survival of
V. cholerae
in amoeba. We also report that
V. cholerae
KatB and KatG catalases confer an increased tolerance to oxidative stress. We conclude that the increased survival of the
flrA
mutant in amoeba is due to a combination of factors, including resistance to oxidative stress and an increased capacity to utilize essential nutrients such as iron and amino acids. Together, the results presented here detail how a bacterial pathogen increases resistance to protozoan predation, thereby allowing for increased survival in the environment.

IMPORTANCE

Persistence of
V. cholerae
in the aquatic environment contributes to the fatal diarrheal disease cholera, which remains a global health burden. In the environment, bacteria face predation pressure by heterotrophic protists such as the free-living amoeba
A. castellanii
. This study explores how a mutant of
V. cholerae
adapts to acquire essential nutrients and survive predation. Here, we observed that up-regulation of iron acquisition genes and genes regulating resistance to oxidative stress enhances pathogen fitness. Our data show that
V. cholerae
can defend predation to overcome nutrient limitation and oxidative stress, resulting in an enhanced survival inside the protozoan hosts.
Channelrhodopsins: From Phototaxis to Optogenetics
Authors: Govorunova Elena G. , Sineshchekov Oleg A. 
Published: October 2023

Abstract
Channelrhodopsins stand out among other retinal proteins because of their capacity to generate passive ionic currents following photoactivation. Owing to that, channelrhodopsins are widely used in neuroscience and cardiology as instruments for optogenetic manipulation of the activity of excitable cells. Photocurrents generated by channelrhodopsins were first discovered in the cells of green algae in the 1970s. In this review we describe this discovery and discuss the current state of research in the field.
Biomarkers of the End-Stage Renal Disease Progression: Beyond the GFR
Authors: Yakupova Elmira I. , Abramicheva Polina A. , Bocharnikov Alexey D. , Andrianova Nadezda V. , Plotnikov Egor Y. 
Published: October 2023

Abstract
Chronic kidney disease can progress to the end-stage renal disease (ESRD) characterized by a high risk of morbidity and mortality. ESRD requires immediate therapy or even dialysis or kidney transplantation, therefore, its timely diagnostics is critical for many patients. ESRD is associated with pathological changes, such as inflammation, fibrosis, endocrine disorders, and epigenetic changes in various cells, which could serve as ESRD markers. The review summarizes information on conventional and new ESRD biomarkers that can be assessed in kidney tissue, blood, and urine. Some biomarkers are specific to a particular pathology, while others are more universal. Here, we suggest several universal inflammatory, fibrotic, hormonal, and epigenetic markers indicative of severe deterioration of renal function and ESRD progression for improvement of ESRD diagnostics.
Modification of Lugano criteria by pre-infusion tumor kinetics improves early survival prediction for patients with lymphoma under chimeric antigen receptor T-cell therapy
Authors: Winkelmann Michael , Blumenberg Viktoria , Rejeski Kai , Quell Christina , Bücklein Veit , Ingenerf Maria , Unterrainer Marcus , Schmidt Christian , Dekorsy Franziska J , Bartenstein Peter , Ricke Jens , von Bergwelt-Baildon Michael , Subklewe Marion , Kunz Wolfgang G 
Published: October 2023

BackgroundChimeric antigen receptor T-cell therapy (CART) is effective for patients with refractory or relapsed lymphoma with prolongation of survival. We aimed to improve the prediction of Lugano criteria for overall survival (OS) at 30-day follow-up (FU1) by including the pre-infusion tumor growth rate (TGRpre-BL) and its early change to 30-day FU1 imaging (TGRpost-BL).MethodsConsecutive patients with pre-baseline (pre-BL), baseline (BL) and FU1 imaging with CT or positron emission tomography/CT before CART were included. TGR was defined as change of Lugano criteria-based tumor burden between pre-BL, BL and FU1 examinations in relation to days between imaging examinations. Overall response and progression-free survival were determined based on Lugano criteria. Proportional Cox regression analysis studied association of TGR with OS. For survival analysis, OS was analyzed using Kaplan-Meier survival curves.ResultsFifty-nine out of 81 patients met the inclusion criteria. At 30-day FU1 8 patients (13.6%) had a complete response (CR), 25 patients (42.4%) a partial response (PR), 15 patients (25.4%) a stable disease (SD), and 11 patients (18.6%) a progressive disease (PD) according to CT-based Lugano criteria. The median TGRpre-BLwas −0.6 mm2/day, 24.4 mm2/day, −5.1 mm2/day, and 18.6 mm2/day and the median TGRpost-BLwas −16.7 mm2/day, −102.0 mm2/day, −19.8 mm2/day and 8.5 mm2/day in CR, PR, SD, and PD patients, respectively. PD patients could be subclassified into a cohort with an increase in TGR (7 of 11 patients (64%), PD TGRpre-to-post-BL INCR) and a cohort with a decrease in TGR (4 of 11 patients (36%), PD TGRpre-to-post-BL DECR) from pre-BL to post-BL. PD TGRpre-to-post-BL DECRpatients exhibited similar OS to patients classified as SD, while PD TGRpre-to-post-BL INCRpatients had significantly shorter OS (65 days vs 471 days, p<0.001).ConclusionIn the context of CART, the additional use of TGRpre-BLand its change to TGRpost-BLdetermined at 30-day FU1 showed better OS prognostication for patients with overall PD according to Lugano criteria. Therefore, this modification of the Lugano classification should be explored as a potential novel imaging biomarker of early response and should be validated prospectively in future studies.
Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) neutralization counteracts T cell immune evasion in breast cancer
Authors: Travelli Cristina , Colombo Giorgia , Aliotta Martina , Fagiani Francesca , Fava Natalia , De Sanctis Rita , Grolla Ambra A , Garcia Joe G N , Clemente Nausicaa , Portararo Paola , Costanza Massimo , Condorelli Fabrizio , Colombo Mario Paolo , Sangaletti Sabina , Genazzani Armando A 
Published: October 2023

BackgroundNicotinamide phosphoribosyltransferase (NAMPT) is a key intracellular enzyme that participates in nicotinamide adenine dinucleotide (NAD) homeostasis as well as a released cytokine (eNAMPT) that is elevated in inflammatory conditions and in cancer. In patients with breast cancer, circulating eNAMPT is elevated and its plasma levels correlate with prognosis and staging. In light of this, we investigated the contribution of eNAMPT in triple negative mammary carcinoma progression by investigating the effect of its neutralization via a specific neutralizing monoclonal antibody (C269).MethodsWe used female BALB/c mice injected with 4T1 clone 5 cells and female C57BL6 injected with EO771 cells, evaluating tumoral size, spleen weight and number of metastases. We injected two times a week the anti-eNAMPT neutralizing antibody and we sacrificed the mice after 28 days. Harvested tumors were analyzed by histopathology, flow cytometry, western blot, immunohistochemistry, immunofluorescence and RNA sequencing to define tumor characteristics (isolating tumor infiltrating lymphocytes and tumoral cells) and to investigate the molecular mechanisms behind the observed phenotype. Moreover, we dissected the functional relationship between T cells and tumoral cells using three-dimensional (3D) co-cultures.ResultsThe neutralization of eNAMPT with C269 led to decreased tumor size and reduced number of lung metastases. RNA sequencing and functional assays showed that eNAMPT controlled T-cell response via the programmed death-ligand 1/programmed cell death protein 1 (PD-L1/PD-1) axis and its neutralization led to a restoration of antitumoral immune responses. In particular, eNAMPT neutralization was able to activate CD8+IFNγ+GrzB+T cells, reducing the immunosuppressive phenotype of T regulatory cells.ConclusionsThese studies indicate for the first time eNAMPT as a novel immunotherapeutic target for triple negative breast cancer.
A dedicated application of evolutionary algorithms: synchrotron X-ray radiation optimization based on an in-vacuum undulator
Authors: Ketenoglu Bora , Bostanci Erkan , Ketenoglu Didem , Canbay Ali Can , Harder Manuel , Karaca Adnan Sahin , Eren Engin , Aydin Ayhan , Yin Zhong , Guzel Mehmet Serdar , Martins Michael 
Published: October 2023

Following the rapid growth in accelerator-based light sources research since the mid of 20th century, miscellaneous third generation synchrotron radiation (SR) facilities such as SSRL, APS, ESRF, PETRA-III, and SPring-8 have come into existence. These SR source facilities provide 1020–1025 photons/s/mrad2/mm2/0.1%BW peak brightness within the photon energy range of 10–105 eV. Since different measurement techniques are utilized at X-ray beamlines of SR facilities, many kinds of insertion devices (i.e., undulators and wigglers) and optical components (e.g., high-resolution monochromators, double-crystal monochromators, lenses, mirrors, etc.) are employed for each experimental setup as a matter of course. Under the circumstances, optimization of a synchrotron beamline is a big concern for many scientists to ensure required radiation characteristics (i.e., photon flux, spot size, photon energy, etc.) for dedicated user experiments. In this respect, an in-vacuum hybrid undulator driven by a 6 GeV synchrotron electron beam is optimized using evolutionary algorithms (EA). Finally, it is shown that EA results are well consistent with both the literature and the analytical calculations, resulting in a promising design estimation for beamline scientists.
Revision of Motec Total Wrist Replacement in the Second Metacarpal
Authors: MAHER Mark , ESTFAN Rami , STOREY Philip , PACKER Greg 
Published: October 2023

Wrist arthroplasty is becoming more commonplace, with various implant choices available. The Motec (Swemac Orthopaedics AB, Linköping, Sweden) cementless ball-and-socket system is being increasingly utilised and is designed for the distal component to be implanted into the third metacarpal. However, distal component failure is a recognised complication. We outline our experience with the revision of this component into the second metacarpal. This technical note is presented through our experience of two patients who underwent revision arthroplasty for the management of peri-prosthetic fracture of the third metacarpal. This technique has demonstrated a safe and viable solution to this complication, achieving good anatomical centre of rotation, function, range of movement and patient satisfaction. Level of Evidence: Level V (Therapeutic)
Angioleiomyoma – A Case Report
Authors: AMRUTIYA Pratik Arvind , BROWN Oliver Scott , PAPANIKOS Efstratios , MALLINA Ravi 
Published: October 2023

Angioleiomyoma is a rare benign soft tissue tumour arising from smooth muscle, representing <1% of upper limb soft tissue tumours. We report a 54-year-old male presenting with a progressively enlarging atraumatic lesion along the palmar side of the base of the ring and little finger. A biopsy was done to determine the diagnosis. Intraoperatively, the lump was found to be intimately related to the radial digital artery, it could not be excised en-bloc without transecting the radial digital artery of the little finger. Following excision, the ends of the digital artery were anastomosed. At 10-months follow-up, the hand was fully functional without any evidence of cold-intolerance or neurological deficit along the distribution of the digital nerve. We review the literature on angioleiomyoma and report careful resection of the tumour with digital artery transection and repair as a treatment option for angioleiomyoma of the digital artery. Level of Evidence: Level V (Therapeutic)