bluuebunny/crossref_metadata_2025_split huggingface dataset:
Benefits and harms of cervical screening, triage and treatment strategies in women living with HIV
Authors: Hall Michaela T. , Simms Kate T. , Murray John M. , Keane Adam , Nguyen Diep T. N. , Caruana Michael , Lui Gigi , Kelly Helen , Eckert Linda O. , Santesso Nancy , de Sanjose Silvia , Swai Edwin E. , Rangaraj Ajay , Owiredu Morkor Newman , Gauvreau Cindy , Demke Owen , Basu Partha , Arbyn Marc , Dalal Shona , Broutet Nathalie , Canfell Karen 
Published: December 2023

AbstractTo support a strategy to eliminate cervical cancer as a public health problem, the World Health Organisation (WHO) reviewed its guidelines for screening and treatment of cervical pre-cancerous lesions in 2021. Women living with HIV have 6-times the risk of cervical cancer compared to women in the general population, and we harnessed a model platform (‘Policy1-Cervix-HIV’) to evaluate the benefits and harms of a range of screening strategies for women living with HIV in Tanzania, a country with endemic HIV. Assuming 70% coverage, we found that 3-yearly primary HPV screening without triage would reduce age-standardised cervical cancer mortality rates by 72%, with a number needed to treat (NNT) of 38.7, to prevent a cervical cancer death. Triaging HPV positive women before treatment resulted in minimal loss of effectiveness and had more favorable NNTs (19.7–33.0). Screening using visual inspection with acetic acid (VIA) or cytology was less effective than primary HPV and, in the case of VIA, generated a far higher NNT of 107.5. These findings support the WHO 2021 recommendation that women living with HIV are screened with primary HPV testing in a screen-triage-and-treat approach starting at 25 years, with regular screening every 3–5 years.
Talin and kindlin use integrin tail allostery and direct binding to activate integrins
Authors: Aretz Jonas , Aziz Masood , Strohmeyer Nico , Sattler Michael , Fässler Reinhard 
Published: December 2023

AbstractIntegrin affinity regulation, also termed integrin activation, is essential for metazoan life. Although talin and kindlin binding to the β-integrin cytoplasmic tail is indispensable for integrin activation, it is unknown how they achieve this function. By combining NMR, biochemistry and cell biology techniques, we found that talin and kindlin binding to the β-tail can induce a conformational change that increases talin affinity and decreases kindlin affinity toward it. We also discovered that this asymmetric affinity regulation is accompanied by a direct interaction between talin and kindlin, which promotes simultaneous binding of talin and kindlin to β-tails. Disrupting allosteric communication between the β-tail-binding sites of talin and kindlin or their direct interaction in cells severely compromised integrin functions. These data show how talin and kindlin cooperate to generate a small but critical population of ternary talin–β-integrin–kindlin complexes with high talin–integrin affinity and high dynamics.
Research and engineering practice on space characteristics of gangue slurry filling
Authors: Gu Wenzhe , Yang Baogui , Pan Hao , Song Tianqi 
Published: December 2023

AbstractThe macroscopic characteristics of the fractured space and the microscopic pore features are critical factors determining the effectiveness of gangue slurry backfilling. To identify the key areas for slurry backfilling, a combination of theoretical analysis, simulation experiments, and on-site industrial trials was used to reveal the movement laws of backfilling space overlying the fractured zone. The distribution characteristics of voids available for slurry backfilling within the fractured space were explored, and the interaction between gangue slurry and goaf voids was clarified. A formula for calculating the void ratio in the caved zone was derived, and a model for the distribution of voids in the slurry backfilling space was established. This model identified the main areas where slurry backfilling should be focused, namely the overlapping space between the free accumulation zone and the load-influenced zone. Experimental results demonstrated that the porosity of the collapsed rock mass within the goaf follows a negative logarithmic function along both the dip and strike directions, which was consistent with the theoretical calculations. Through in-situ grouting backfilling experiments on the ground, the feasibility of gangue slurry backfilling in the goaf was verified, and the process of interaction between gangue slurry and goaf voids was elaborated. This process generally involves three stages: initial flow, vertical upwelling, and horizontal diffusion, all of which are closely interconnected. Practical engineering applications of gangue slurry backfilling were carried out in the free accumulation zone and the load-influenced zone at the Huangling No. 2 coal mine. The successful validation of underground gangue slurry backfilling technology demonstrated its feasibility and the correctness of the theoretical approach. This research provides new evidence for environmentally friendly disposal of gangue materials.
Structural and biophysical comparisons of the pomalidomide- and CC-220-induced interactions of SALL4 with cereblon
Authors: Ma Xiaolei , Leon Barbara , Ornelas Elizabeth , Dovala Dustin , Tandeske Laura , Luu Catherine , Pardee Gwynn , Widger Stephania , Solomon Jonathan M. , Beckwith Rohan E. J. , Moser Heinz , Clifton Matthew C. , Wartchow Charles A. 
Published: December 2023

AbstractThe design of cereblon-binding molecular glues (MGs) that selectively recruit a desired protein while excluding teratogenic SALL4 is an area of significant interest when designing therapeutic agents. Previous studies show that SALL4 is degraded in the presence of IKZF1 degraders pomalidomide, and to a lesser extent by CC-220. To expand our understanding of the molecular basis for the interaction of SALL4 with cereblon, we performed biophysical and structural studies demonstrating that SALL4 zinc finger domains one and two (ZF1-2) interact with cereblon (CRBN) in a unique manner. ZF1 interacts with the N-terminal domain of cereblon and ZF2 binds as expected in the C-terminal IMiD-binding domain. Both ZF1 and ZF2 contribute to the potency of the interaction of ZF1-2 with CRBN:MG complexes and the affinities of SALL4 ZF1-2 for the cereblon:CC-220 complex are less potent than for the corresponding pomalidomide complex. Structural analysis provides a rationale for understanding the reduced affinity of SALL4 for cereblon in the presence of CC-220, which engages both ZF1 and ZF2. These studies further our understanding of the molecular glue-mediated interactions of zinc finger-based proteins with cereblon and may provide structural tools for the prospective design of compounds with reduced binding and degradation of SALL4.
DC-electrical penetration graph waveforms for Dalbulus maidis (Hemiptera: Cicadellidae) and the effects of entomopathogenic fungi on its probing behavior
Authors: Maluta Nathalie , Castro Thiago , Spotti Lopes João Roberto 
Published: December 2023

AbstractThe corn leafhopper Dalbulus maidis is an important transmitter of viruses and bacteria to maize plants. Data on the stylet probing and ingestion behavior of D. maidis, gathered using the DC-electrical penetration graph (DC-EPG) technique, are limited. The increasing prevalence of this pest and the diseases that it transmits in maize crops heighten the importance of studying how control tools affect the probing behavior of these insects, to reduce or prevent the chances of pathogen transmission and the damage from feeding. Our study recorded stylet activities of D. maidis, using a DC-EPG system and compared the appearances of waveforms with those from published AC and AC-DC EPG studies. All types of systems produced similar waveform appearances; therefore, we used the waveform nomenclature previously published. We also determined the effects of the entomopathogenic fungus Cordyceps javanica on the probing behavior of D. maidis at different time points after the fungus was applied by spraying. Forty-eight hours after the insects were sprayed, the effects were pronounced, with significant disruption of the stylet activities in phloem and non-phloem phases. Our study indicated that this commercial microbiological product, with the active ingredient C. javanica, can alter the probing behavior of D. maidis and may be helpful in management of the vector.
Associations between recollected rates of Category D fruit machine use as a child and adult gambling in a young adult sample
Authors: Newall Philip 
Published: December 2023

AbstractThere have been recent restrictions to the range of gambling products made available to UK children, with the industry association Bacta introducing a minimum age of 18 for Category D fruit machines, which can otherwise be legally used by children. Any potential government action on Category D fruit machines may want to consider limitations in the current evidence base. The present study addressed this issue, by conceptually replicating previous results associating recollected usage of Category D fruit machine usage as a child and adult gambling. Compared to previous studies, the present study used a younger adult sample, and also tested for potential moderation by subjective confidence. Three out of four main tested associations were significant and in the hypothesised direction, and a lack of significance on interaction terms yielded no evidence of potential moderation by subjective confidence. The present study therefore added to the limited evidence base on potential risks of legal Category D fruit machine usage as a child.
Calcium phosphate ceramics combined with rhBMP6 within autologous blood coagulum promote posterolateral lumbar fusion in sheep
Authors: Ivanjko Natalia , Stokovic Nikola , Pecin Marko , Vnuk Drazen , Smajlovic Ana , Ivkic Niko , Capak Hrvoje , Javor Ana , Vrbanac Zoran , Maticic Drazen , Vukicevic Slobodan 
Published: December 2023

AbstractPosterolateral spinal fusion (PLF) is a procedure used for the treatment of degenerative spine disease. In this study we evaluated Osteogrow-C, a novel osteoinductive device comprised of recombinant human Bone morphogenetic protein 6 (rhBMP6) dispersed in autologous blood coagulum with synthetic ceramic particles, in the sheep PLF model. Osteogrow-C implants containing 74–420 or 1000–1700 µm ceramic particles (TCP/HA 80/20) were implanted between L4–L5 transverse processes in sheep (Ovis Aries, Merinolaandschaf breed). In the first experiment (n = 9 sheep; rhBMP6 dose 800 µg) the follow-up period was 27 weeks while in the second experiment (n = 12 sheep; rhBMP6 dose 500 µg) spinal fusion was assessed by in vivo CT after 9 weeks and at the end of the experiment after 14 (n = 6 sheep) and 40 (n = 6 sheep) weeks. Methods of evaluation included microCT, histological analyses and biomechanical testing. Osteogrow-C implants containing both 74–420 and 1000–1700 µm ceramic particles induced radiographic solid fusion 9 weeks following implantation. Ex-vivo microCT and histological analyses revealed complete osseointegration of newly formed bone with adjacent transverse processes. Biomechanical testing confirmed that fusion between transverse processes was complete and successful. Osteogrow-C implants induced spinal fusion in sheep PLF model and therefore represent a novel therapeutic solution for patients with degenerative disc disease.
Effect of Pd/Pt decoration on MoSSe monolayer for CH4 signature through surface adsorption mechanism
Authors: Babariya Bindiya , Gupta Sanjeev K. , Gajjar P. N. 
Published: December 2023

AbstractConsidering the current breakthrough in gas sensor technology, we have examined impact of CH4 in the vicinity of pristine MoSSe and Pd/Pt decorated MoSSe monolayers using first principles approach. The negative formation energies confirm structural stability of considered monolayers. The pristine MoSSe monolayer is semiconductor having 1.52 eV direct band gap. This value decreases in the presence of Pd/Pt adatom. Further, adsorption strength of CH4 to monolayers is validated by sensing parameters such as adsorption energy, recovery time, charge transfer and work function. Though we found maximum adsorption energies of − 0.674 and − 0.636 eV for adsorption on Se site of Pd/Pt decorated MoSSe monolayers, the overall sensing response also reveals high sensitivity for Se surface. However, both sites S and Se are favorable for CH4 adsorption. When CH4 is activated on Pd/Pt decorated monolayers, band gaps vary with marginal alterations and transform to direct type. Moreover, optical dielectric response alters strongly in the visible region after activation of CH4 on to Pd/Pt decorated MoSSe monolayers. This result identifies sensitivity response in the presence of methane which may detect CH4 gas easily in visible region. Generally, these interesting results of methane sensing study provoke Pd/Pt decorated MoSSe monolayers to be good sensing nano-device.
Research on the localized lightfield features of metallic nano-cone-tip optical antenna via investigating near-field lightwave and correlated net-charge distribution
Authors: Liu Taige , Shi Jiashuo , Duan Shenghua , Ji Wuyang , Wang Zhe , Zhang Xinyu 
Published: December 2023

AbstractIn this paper, the near-field lightwave characteristics of an arrayed silicon nano-cone-tip optical antenna (NOA) covered by a common metal film, which can be viewed as a featured quasi quantum dot (QD), are carefully investigated. A dipole net-charge distribution closely correlated with the surface lightwaves excited over the antennas by incident lasers with a central wavelength of 633 nm, is clearly observed. An obvious Coulomb-like blockade from the apex apparently influencing the net-charge converging over the surface of NOA, is verified, which can also be predicted by the simulations according to surface standing waves across the apex node. The antinodes of the surface net-charge instantaneous distribution are already pushed away from the normal location owing to the apex Coulomb-like blockade, so as to present a distorted waveform different from traditional standing wave modes. The tip proximity effect leading to a relatively weak net-charge converging over surrounding planar facet and adjacent NOAs, is also discovered.
Characterization of durum wheat resistance against leaf rust under climate change conditions of increasing temperature and [CO2]
Authors: Porras Rafael , Miguel-Rojas Cristina , Lorite Ignacio J. , Pérez-de-Luque Alejandro , Sillero Josefina C. 
Published: December 2023

AbstractDurum wheat cultivation in Mediterranean regions is threatened by abiotic factors, mainly related to the effects of climate change, and biotic factors such as the leaf rust disease. This situation requires an in-depth knowledge of how predicted elevated temperatures and [CO2] will affect durum wheat-leaf rust interactions. Therefore, we have characterised the response of one susceptible and two resistant durum wheat accessions against leaf rust under different environments in greenhouse assays, simulating the predicted conditions of elevated temperature and [CO2] in the far future period of 2070–2099 for the wheat growing region of Cordoba, Spain. Interestingly, high temperature alone or in combination with high [CO2] did not alter the external appearance of the rust lesions. However, through macro and microscopic evaluation, we found some host physiological and molecular responses to infection that would quantitatively reduce not only pustule formation and subsequent infection cycles of this pathogen, but also the host photosynthetic area under these predicted weather conditions, mainly expressed in the susceptible accession. Moreover, our results suggest that durum wheat responses to infection are mainly driven by temperature, being considered the most hampering abiotic stress. In contrast, leaf rust infection was greatly reduced when these weather conditions were also conducted during the inoculation process, resembling the effects of possible heat waves not only in disease development, but also in fungal germination and penetration success. Considering this lack of knowledge in plant-pathogen interactions combined with abiotic stresses, the present study is, to the best of our knowledge, the first to include the effects of the expected diurnal variation of maximum temperature and continuous elevated [CO2] in the durum wheat-leaf rust pathosystem.
Level I of evidence does not support manual lymphatic drainage for total knee arthroplasty: a meta-analysis
Authors: Migliorini Filippo , Schäfer Luise , Bertini Francesca Alzira , Memminger Michael Kurt , Simeone Francesco , Giorgino Riccardo , Maffulli Nicola 
Published: December 2023

AbstractIt is unclear whether manual lymphatic drainage (MLD) following primary total knee arthroplasty (TKA) is effective in reducing pain and swelling and improving knee function. The present study investigated the efficacy of MLD after TKA. The outcomes of interest are the range of motion (ROM), pain (visual analogue scale, VAS), and circumference of the lower leg. This meta-analysis was conducted according to the 2020 PRISMA statement. In November 2023, the following databases were accessed: PubMed, Web of Science, Google Scholar, and Embase, with no time constraint. Only level I evidence studies, according to the Oxford Centre of Evidence-Based Medicine, were considered. All the randomised controlled trials (RCTs) comparing patients who have received MLD versus a group of patients who did not undergo MLD following primary TKA were accessed. Data from four RCTs (197 TKAs) were retrieved. 67% (132 of 197 patients) were women. The mean length of follow-up was 7.0 ± 5.8 weeks. The mean age of the patients was 69.6 ± 2.7 years, and the mean BMI was 28.7 ± 0.9 kg/m2. At baseline, between-group comparability was evidenced in the male:female ratio, mean age, mean BMI, knee flexion, and VAS. No difference was found in flexion (P = 0.7) and VAS (P = 0.3). No difference was found in the circumference of the thigh (P = 0.8), knee (P = 0.4), calf (P = 0.4), and ankle (P = 0.3). The current level I of evidence does not support the use of MLD in primary TKA.
A noval pulmonary function evaluation method based on ResNet50 + SVR model and cough
Authors: Xu Wenlong , He Guoqiang , Shen Dan , Xu Bingqiao , Jiang Peirong , Liu Feng , Lou Xiaomin , Guo Lingling , Ma Li 
Published: December 2023

AbstractTraditionally, the clinical evaluation of respiratory diseases was pulmonary function testing, which can be used for the detection of severity and prognosis through pulmonary function parameters. However, this method is limited by the complex process, which is impossible for patients to monitor daily. In order to evaluate pulmonary function parameters conveniently with less time and location restrictions, cough sound is the substitute parameter. In this paper, 371 cough sounds segments from 150 individuals were separated into 309 and 62 as the training and test samples. Short-time Fourier transform (STFT) was applied to transform cough sound into spectrogram, and ResNet50 model was used to extract 2048-dimensional features. Through support vector regression (SVR) model with biological attributes, the data were regressed with pulmonary function parameters, FEV1, FEV1%, FEV1/FVC, FVC, FVC%, and the performance of this models was evaluated with fivefold cross-validation. Combines with deep learning and machine learning technologies, the better results in the case of small samples were achieved. Using the coefficient of determination (R2), the ResNet50 + SVR model shows best performance in five basic pulmonary function parameters evaluation as FEV1(0.94), FEV1%(0.84), FEV1/FVC(0.68), FVC(0.92), and FVC%(0.72). This ResNet50 + SVR hybrid model shows excellent evaluation of pulmonary function parameters during coughing, making it possible to realize a simple and rapid evaluation for pneumonia patients. The technology implemented in this paper is beneficial in judge the patient's condition, realize early screening of respiratory diseases, evaluate postoperative disease changes and detect respiratory infectious diseases without time and location restrictions.
Stability of Feline Coronavirus in aerosols and dried in organic matrices on surfaces at various environmental conditions
Authors: Reissner Janina , Siller Paul , Bartel Alexander , Roesler Uwe , Friese Anika 
Published: December 2023

AbstractEnveloped respiratory viruses, including the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), can be transmitted through aerosols and contact with contaminated surfaces. The stability of these viruses outside the host significantly impacts their transmission dynamics and the spread of diseases. In this study, we investigated the tenacity of Feline Coronavirus (FCoV) in aerosols and on surfaces under varying environmental conditions. We found that airborne FCoV showed different stability depending on relative humidity (RH), with higher stability observed at low and high RH. Medium RH conditions (50–60%) were associated with increased loss of infectivity. Furthermore, FCoV remained infectious in the airborne state over 7 h. On stainless-steel surfaces, FCoV remained infectious for several months, with stability influenced by organic material and temperature. The presence of yeast extract and a temperature of 4 °C resulted in the longest maintenance of infectivity, with a 5 log10 reduction of the initial concentration after 167 days. At 20 °C, this reduction was achieved after 19 days. These findings highlight the potential risk of aerosol and contact transmission of respiratory viruses, especially in enclosed environments, over extended periods. Studying surrogate viruses like FCoV provides important insights into the behavior of zoonotic viruses like SARS-CoV-2 in the environment.
Investigating the prognostic value of mTORC1 signaling in bladder cancer via bioinformatics evaluation
Authors: Yu Xin , Li Wenge , Sun Shengrong , Li Juanjuan 
Published: December 2023

AbstractBladder cancer, a prevalent and heterogeneous malignancy, necessitates the discovery of pertinent biomarkers to enable personalized treatment. The mammalian target of rapamycin complex 1 (mTORC1), a pivotal regulator of cellular growth, metabolism, and immune response, exhibits activation in a subset of bladder cancer tumors. In this study, we explore the prognostic significance of mTORC1 signaling in bladder cancer through the utilization of bioinformatics analysis. Our investigation incorporates transcriptomic, somatic mutation, and clinical data, examining the mTORC1 score of each sample, as well as the enrichment of differentially expressed genes (DEGs), differentiation characteristics, immunological infiltration, and metabolic activity. Our findings reveal that elevated mTORC1 levels serve as an adverse prognostic indicator for bladder cancer patients, exhibiting a significant association with Basal-type bladder cancer. Patients with heightened mTORC1 activation display heightened levels of pro-carcinogenic metabolism. Additionally, these individuals demonstrate enhanced response to immunotherapy. Finally, we develop an mTORC1-related signature capable of predicting the prognosis of bladder cancer patients.The signature offers novel mTORC1-related biomarkers and provides fresh insights into the involvement of mTORC1 in the pathogenesis of bladder cancer.