bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reproduction cycle, trematode parasitism and growth of freshwater mussel Anodonta anatina (Bivalvia: Unionidae)
Authors: Abdelsaleheen Olfat , Taskinen Jouni , Kekäläinen Jukka , Kortet Raine 
Published: December 2023

AbstractAnodonta anatina, a widely distributed Eurasian freshwater mussel, has dramatically declined and listed as endangered species in IUCN Red List in some parts of the Europe. To better understand its basic biology and possible sex-dependent effects of parasitism on reproduction, we investigated the reproductive traits, trematode parasitism and growth between November 2020 and October 2021. Our data from a Finnish population indicates male-biased sex ratio and the gonadal development lasting throughout the year with a bradytictic brooding pattern. Gonadosomatic index of both sexes peaked in May and declined in June. Mature oocytes were smaller sized than those of previously studied unionids, fecundity was fairly low, and the maximum predicted lifespan was 14 years. Gonads were infected with digenean trematode parasites, Rhipidocotyle campanula being the dominant species. Interestingly, trematode infection rate was higher in females than in males. In July, when the water temperature reached its annual maximum, the trematode prevalence also peaked, which was accompanied with 16% of mussels being sterile. The trematode parasitism was thus negatively associated with reproduction, that might become even more affected if parasitism increases along with the predicted climate warming, which should be considered in possible future conservation and aquaculture efforts of this species.
Biodiversity patterns of cyanobacterial oligotypes in lakes and rivers: results of a large-scale metabarcoding survey in the Alpine region
Authors: Salmaso Nico , Bernabei Serena , Boscaini Adriano , Capelli Camilla , Cerasino Leonardo , Domaizon Isabelle , Elersek Tina , Greco Claudia , Krivograd Klemenčič Aleksandra , Tomassetti Paolo , Kurmayer Rainer 
Published: December 2023

AbstractIn this work, we characterised the cyanobacterial communities in the plankton and littoral biofilm of 38 lakes and in the biofilm of 21 rivers in the Alps and surrounding subalpine regions by 16S rRNA gene metabarcoding. We found little overlap in the distribution of amplicon sequence variants (ASVs) between the three habitats and between water bodies. The differences were caused by environmental filtering acting on the selection of the most abundant ASVs and a high contribution of rare oligotypes. The differentiation of community and genotype composition from specific water bodies was explained to a significant extent by environmental variables and morphometry. The taxonomic consistency of ASVs classified under the same genus name was assessed by phylogenetic analyses performed on three representative dominant genera, namely Cyanobium, Tychonema and Planktothrix. The analyses revealed eco-evolutionary adaptations in lakes and rivers, including some evidence for a polyphyletic nature. Monitoring individual genotypes in relation to environmental conditions will be useful to define the ecological amplitude of these taxa. However, the persistence or ephemeral nature of some of the rarest and most unusual ASVs has remained unknown.
The role of the microstructural changes during induction preheating on the HAZ liquation cracking susceptibility of Ni-based superalloy
Authors: Rakoczy Łukasz , Grudzień-Rakoczy Małgorzata , Rutkowski Bogdan , Cygan Rafał , Zielińska-Lipiec Anna 
Published: December 2023

AbstractThis work presents the influence of high-frequency induction preheating (900, 1000, 1100 °C) on liquation crack formation in the René 108 Ni-based superalloy. The investigation was divided into two parts: (1) characterization of the material's microstructure after preheating and (2) determining the influence of preheating on liquation cracking during autogenous gas tungsten arc welding. During preheating, the dissolution of γ′ precipitates showed accelerated progress with increase in temperatures. This dissolution involved the continuous thinning of each precipitate, as well as more intricate mechanisms, such as splitting. The mean size of the secondary γ′ decreased from 0.32 to 0.26 μm. In the heat-affected zone (HAZ) induced by welding, constitutional liquation of mainly γ' precipitates, with a contribution of M23C6 carbides and M5B3 borides, was observed. The formation of a thin non-equilibrium liquid film along high-angle grain boundaries led to the crack initiation and their further propagation during cooling. The eutectic γ–γ' re-solidification products were visible on the crack edges independently of preheat temperature. Preheating at 900 °C decreased the length and amount of liquation cracks, while preheating at 1100 °C allowed to prevent them due to the liquid-healing effect.
Structural and magnetic properties of SmCo/Co nanocomposites elaborated using sol–gel auto-combustion strategy
Authors: Emira Sayed , Shaaban E. R. , Gelany Shaker A. , Rashad M. M. 
Published: December 2023

AbstractSm–Co nanomagnetic material has received much attention recently since it is thought to be the next generation of permanent magnets with potential uses in energy technologies. Here, ethylenediaminetetraacetic acid (EDTA) is utilized for the first time as a fuel source in a sol–gel auto-combustion process to synthesize Sm–Co nanoparticles. Then, reduction–diffusion process strategy followed the auto-combustion pathway. Typically, Sm2O3 and Co3O4 nanoparticles were prepared by combining Sm and Co nitrates with the chelating agent EDTA. The Sm–Co nanocomposites were subsequently created by reductively annealing precursor oxides using calcium powder. To display the temperature-dependent breakdown of the original precursor and determine the correct annealing temperature, TGA was employed to identify the annealing temperature and the precursor products. Additionally, other physical characterization techniques such as XRD, FE-SEM, EDX, and VSM were used for further investigations. Three distinct Sm1Cox compositions with different cobalt ratios (x = 4.0, 3.5, and 2.0) were prepared and studied. The findings demonstrate that the composition Sm1Cox (x = 2.0) led to the formation of hard phases of SmCo5, Sm2Co7, and Sm2Co17. These particles’ morphology reveals that they are made up of nanowires with an average thickness of 25 nm. As well, according to the VSM findings, this composite had the highest coercivity Hc and a maximum squareness ratio Mr/Ms, which were 2161 Oe and 0.57, respectively.
Coupling homogeneous chains of damped harmonic oscillators
Authors: Bandeira Luís , Correia Ramos Carlos 
Published: December 2023

AbstractWe study vibrational properties of non-homogeneous materials. We consider idealized materials composed of one dimensional chains of damped harmonic oscillators represented by a sequence of particles, springs and dampers. The system is linear, thus possesses exact explicit solutions, nevertheless the formulas can be very complicated. Homogeneous chains are used as basic building blocks for characterizing global dynamics of the system. In particular, we determine the solutions for a system composed of two distinct homogeneous chains in terms of the original solutions for these two homogeneous chains, and the original parameters, when uncoupled. Two types of coupling are considered, one with a gluing particle, the other with a gluing spring and damper.
The role of HIF in angiogenesis, lymphangiogenesis, and tumor microenvironment in urological cancers
Authors: Lin Shen , Chai Yueyang , Zheng Xiangyi , Xu Xin 
Published: December 2023

Abstract
Typically associated with solid tumors, hypoxia contributes to tumor angiogenesis and lymphangiogenesis through various molecular mechanisms. Accumulating studies indicate that hypoxia-inducible factor is the key transcription factor coordinating endothelial cells to respond to hypoxia in urological cancers, mainly renal cell carcinoma, prostate cancer, and bladder cancer. Moreover, it has been suggested that tumor hypoxia in tumor microenvironment simultaneously recruits stromal cells to suppress immune activities. This review summarizes the mechanisms by which HIF regulates tumorigenesis and elaborates on the associations between HIF and angiogenesis, lymphangiogenesis, and tumor microenvironment in urological cancers.
Changes in miRNA expression in the lungs of pigs supplemented with different levels and forms of vitamin D
Authors: Wierzbicka Alicja , Pawlina-Tyszko Klaudia , Świątkiewicz Małgorzata , Szmatoła Tomasz , Oczkowicz Maria 
Published: December 2023

Abstract

Background
Vitamin D is an immunomodulator, and its effects have been linked to many diseases, including the pathogenesis of cancer. However, the effect of vitamin D supplementation on the regulation of gene expression of the lungs is not fully understood. This study aims to determine the effect of the increased dose of cholecalciferol and a combination of cholecalciferol + calcidiol, as well as the replacement of cholecalciferol with calcidiol, on the miRNA profile of healthy swine lungs.

Methods and results
The swine were long-term (88 days) supplemented with a standard dose (2000IU/kg) of cholecalciferol and calcidiol, the increased dose (3000 IU/kg) of cholecalciferol, and the cholecalciferol + calcidiol combination: grower: 3000 IU/Kg of vitamin D (67% of cholecalciferol and 33% of calcidiol), finisher 2500 IU/Kg of vitamin D (60% of cholecalciferol and 40% of calcidiol). Swine lung tissue was used for Next Generation Sequencing (NGS) of miRNA. Long-term supplementation with the cholecalciferol + calcidiol combination caused significant changes in the miRNA profile. They embraced altered levels of the expression of miR-150, miR-193, miR-145, miR-574, miR-340, miR-381, miR-148 and miR-96 (q-value < 0.05). In contrast, raising the dose of cholecalciferol only changed the expression of miR-215, and the total replacement of cholecalciferol with calcidiol did not significantly affect the miRNAome profile.

Conclusions
The functional analysis of differentially expressed miRNAs suggests that the use of the increased dose of the cholecalciferol + calcidiol combination may affect tumorigenesis processes through, inter alia, modulation of gene regulation of the TGF- β pathway and pathways related to metabolism and synthesis of glycan.
Drought and deluge: the recurrence of hydroclimate extremes during the past 600 years in eastern Australia’s Natural Resource Management (NRM) clusters
Authors: Palmer Jonathan G. , Verdon-Kidd Danielle , Allen Kathryn J. , Higgins Philippa , Cook Benjamin I. , Cook Edward R. , Turney Christian S. M. , Baker Patrick J. 
Published: December 2023

AbstractRecent extremes of flood and drought across Australia have raised questions about the recurrence of such rare events and highlighted the importance of understanding multi-decadal climate variability. However, instrumental records over the past century are too short to adequately characterise climate variability on multi-decadal and longer timescales or robustly determine extreme event frequencies and their duration. Palaeoclimate reconstructions can provide much-needed information to help address this problem. Here, we use the 600-year hydroclimate record captured in the eastern Australian and New Zealand Drought Atlas (ANZDA) to analyse drought and pluvial frequency trends for East Australian Natural Resource Management (NRM) clusters. This partitioning of the drought atlas grid points into recognised biophysical areas (i.e. NRM clusters) revealed their differences and similarities in drought intensity and pluvial events over time. We find sustained multi-decadal periods of a wet–dry geographic 'seesaw' between eastern to central and southern NRMs (e.g. 1550–1600 CE and 1700–1750 CE). In contrast, other periods reveal spatially consistent wetting (e.g. 1500–1550 CE) or drying (e.g. 1750–1800 CE). Emerging hot spot analysis further shows that some areas that appear naturally buffered from severe drought during the instrumental period have a greater exposure risk when the longer 600-year record is considered. These findings are particularly relevant to management plans when dealing with the impacts of climate extremes developed at regional scales. Our results demonstrate that integrating and extending instrumental records with palaeoclimate datasets will become increasingly important for developing robust and locally specific extreme event frequency information for managing water resources.
Australian population norms for health-related quality of life measured using the EQ-5D–5L, and relationships with sociodemographic characteristics
Authors: Redwood Lisa , Currow David , Kochovska Slavica , Thomas Susan J. 
Published: December 2023

Abstract
Background
Measuring health related quality-of-life (HRQoL) of the general population is essential to establish a reference for health outcome evaluations. This study sought to establish EQ-5D-5L population norms in Australia and to investigate the heterogeneity of HRQoL between sociodemographic variables.

Methods
A cross-sectional study comprising of a representative sample of Australia’s general population (n = 9958) aged 18 or older. Recruitment quotas were set for the Australian census population by age, sex, state/territory of residence and rurality. Participants were recruited by Qualtrics through its database of over 800,000 registered panel members and asked to value their own state of health using the EQ-5D-5L domains and the EuroQol-Visual Analogue Scale (EQ-VAS). An Australian value set developed using Discreet Choice Experiment was used to calculate utility scores.

Results
The estimated mean EQ-5D-5L index for Australia’s general population was 0.86 (standard deviation [SD] 0.19), and the EQ-VAS score was estimated as 73.2 (SD 21.7). 23.9% of the study population reported being in the best health state (11,111). Younger people, current smokers, people who are unemployed and people with more financial stress reported a lower EQ-5D-5L index score (p < 0.001). Residents in the major cities, inner regional and outer regional Australia reported higher health utility scores than those residing in remote and very remote Australia.

Conclusions
This is the first Australian study to apply the EQ-5D-5L in a nationally representative sample. The EQ-5D-5L Australian population norms obtained can be used as reference scores for future population health evaluations and comparisons. The findings facilitate a national reference for clinical, economic, and policy decision-making processes and provide a fuller understanding of the Australian population’s HRQoL.
Bio-bearings: Numerical Model of the Solid Lubricant in the Leg Joints of Insects
Authors: Filippov Alexander E. , Nadein Konstantin , Gorb Stanislav N. , Kovalev Alexander 
Published: December 2023

AbstractThe proposed model describes the behavior of the lubricant particles at the interface between two surfaces for a group of cylindrical semi-solid lubricant fragments observed in a real beetle leg joint. It is shown that the lubricant particles may maintain a gap between joint surfaces at load. At the regular shear motion, such randomly oriented particles are reorienting perpendicular to the shear and function as a rolling bearing: the system demonstrates the lowest energy loss and lowest friction. At an increased load, the friction non-linearly increases, because of an increase of the particles’ eccentricity. Finally, at some load, the particle rotation stops, friction switches from the rolling to the sliding regime and increases significantly.
Agents of change or victims of transition? Media framings on household roles during the energy crisis
Authors: Laakso Senja , Castellazzi Eugenia , Matschoss Kaisa , Rinkinen Jenny 
Published: December 2023

AbstractThe energy crisis that began in late 2021 and escalated after the war in Ukraine in early 2022 was seen as a disruption accelerating the energy transition in Europe. It forced households to save energy and change their patterns of energy use in a struggle to cut dependency on Russian energy, and in public discussions, various kinds of agency were required from and allocated to households. In this article, we analyze a total of 220 articles on energy use published in the main Finnish newspaper, Helsingin Sanomat, during the energy crisis between 1 November 2021 and 31 October 2022. We investigate how household agency driven by the energy crisis is framed in the media by analyzing the variety of roles assigned to households and the framings of the agency attached to those roles in relation to mitigating the energy crisis. The analysis provides insights into how households are embedded within the energy system and whether and how they are expected to facilitate the energy transition; moreover, the article contributes to a better conceptualization of the understudied roles and agency of households in transitions.
Rethinking internationalization processes: toward a circular framework
Authors: Domínguez Romero Elena , Durst Susanne , Navarro Garcia Antonio 
Published: December 2023

AbstractWhy should models and theories that have set images and boundaries of the internationalization process in the past remain constant over time within International Business research? Despite a recent emphasis on its nonlinear nature, many still view internationalization as a forward-moving process, overlooking its dynamic nature involving enter, re-enter, and exit decisions during the process. To discuss this issue, this paper rethinks the existing International Business literature to retain its explanatory power as an essential discipline by developing a circular framework to capture firms' non-linear internationalization process. The authors propose a circular framework with the decision-making process as the unit of analysis and four statuses: Enter, De-Internationalization, Re-Internationalization, and Operation Internationalized. The decision-making process guides firms through the statuses simultaneously or individually, in the same or a diverse temporal dimension. With this circular framework, the authors wish to contribute to future International Business research; research that can keep up with the “real” world.
Broad Range Eubacterial Polymerase Chain Reaction of Cerebrospinal Fluid Reduces the Time to Exclusion of and Costs Associated with Ventriculostomy-Related Infection in Hemorrhagic Stroke
Authors: Pietrzko Elisabeth , Bögli Stefan , Frick Katja , Ebner-Dietler Sabeth , Capone Crescenzo , Imkamp Frank , Koliwer-Brandl Hendrik , Müller Nicolas , Keller Emanuela , Brandi Giovanna 
Published: December 2023

Abstract
Background
Patients with hemorrhagic stroke and an external ventricular drain in situ are at risk for ventriculostomy-related-infections (VRI). Because of the contamination of the cerebrospinal fluid (CSF) with blood and the high frequency of false negative CSF culture, the diagnosis of VRI remains challenging. This study investigated the introduction of CSF broad range eubacterial polymerase chain reaction (ePCR) and its effect on frequency and duration of antibiotic therapy for VRI, neurocritical care unit (NCCU) length of stay, related costs, and outcome.

Methods
Between 2020 and 2022, we prospectively included 193 patients admitted to the NCCU of the University Hospital of Zürich with hemorrhagic stroke and an external ventricular drain for more than 48 h. Patient characteristics, serum inflammatory markers, white blood cell count in CSF, use and duration of antibiotic treatment for VRI, microbiological findings (CSF cultures and ePCR tests), and NCCU length of stay were compared in patients with no infection, noncerebral infection, suspected VRI, and confirmed VRI. Data of patients with suspected VRI of this cohort were compared with a retrospective cohort of patients with suspected VRI treated at our NCCU before the introduction of CSF ePCR testing (2013–2019).

Results
Out of 193 patients, 12 (6%) were diagnosed with a confirmed VRI, 66 (34%) with suspected VRI, 90 (47%) with a noncerebral infection, and 25 (13%) had no infection at all. Compared with the retrospective cohort of patients, the use of CSF ePCR resulted in a reduction of patients treated for suspected VRI for the whole duration of 14 days (from 51 to 11%). Furthermore, compared with the retrospective group of patients with suspected VRI (n = 67), after the introduction of CSF ePCR, patients with suspected VRI had shorter antibiotic treatment duration of almost 10 days and, hence, lower related costs with comparable outcome at 3 months.

Conclusions
The use of CSF ePCR to identify VRI resulted in shorter antibiotic treatment duration without changing the outcome, as compared with a retrospective cohort of patients with suspected VRI.
KLF5 promotes esophageal squamous cell cancer through the transcriptional activation of FGFBP1
Authors: Wang Fengyun , Luo Ming , Cheng Yufeng 
Published: December 2023

AbstractKrüpple-like factor 5 (KLF5) is a zinc-finger-containing transcription factor implicated in several human malignancies, but its potential regulatory mechanisms implicated in esophageal squamous cell carcinoma (ESCC) remain elusive. Here, we show that KLF5 is upregulated in ESCC, where its level was significantly associated with tumor differentiation and lymph node metastasis status. Upregulated KLF5 expression promoted the proliferation, migration, and invasion of ESCC cells. Reduced KLF5 showed the opposite effects. Mechanistically, KLF5 exerts its tumor promotion effect by up-regulating fibroblast growth factor binding protein 1 (FGF-BP1) and snail family transcriptional repressor 2 (SNAIL2). KLF5 binds to the promoter regions of FGF-BP1 and transcriptionally activates its expression. Our study indicated that KLF5 could promote esophageal squamous cell cancer proliferation, migration, and invasion by upregulating FGF-BP1/SNAIL2 signaling. Our work suggests that KLF5 might be a proto-oncogene in ESCC and implicated in ESCC metastasis.
Pathological Deficit of Cystatin B Impairs Synaptic Plasticity in EPM1 Human Cerebral Organoids
Authors: Pizzella Amelia , Penna Eduardo , Abate Natalia , Frenna Elisa , Canafoglia Laura , Ragona Francesca , Russo Rosita , Chambery Angela , Perrone-Capano Carla , Cappello Silvia , Crispino Marianna , Di Giaimo Rossella 
Published: December 2023

AbstractCystatin B (CSTB) is a small protease inhibitor protein being involved in cell proliferation and neuronal differentiation. Loss-of-function mutations in CSTB gene cause progressive myoclonic epilepsy 1 (EPM1). We previously demonstrated that CSTB is locally synthesized in synaptic nerve terminals from rat brain and secreted into the media, indicating its role in synaptic plasticity. In this work, we have further investigated the involvement of CSTB in synaptic plasticity, using synaptosomes from human cerebral organoids (hCOs) as well as from rodents’ brain. Our data demonstrate that CSTB is released from synaptosomes in two ways: (i) as a soluble protein and (ii) in extracellular vesicles-mediated pathway. Synaptosomes isolated from hCOs are enriched in pre-synaptic proteins and contain CSTB at all developmental stages analyzed. CSTB presence in the synaptic territories was also confirmed by immunostaining on human neurons in vitro. To investigate if the depletion of CSTB affects synaptic plasticity, we characterized the synaptosomes from EPM1 hCOs. We found that the levels of presynaptic proteins and of an initiation factor linked to local protein synthesis were both reduced in EPM1 hCOs and that the extracellular vesicles trafficking pathway was impaired. Moreover, EPM1 neurons displayed anomalous morphology with longer and more branched neurites bearing higher number of intersections and nodes, suggesting connectivity alterations. In conclusion, our data strengthen the idea that CSTB plays a critical role in the synapse physiology and reveal that pathologically low levels of CSTB may affect synaptic plasticity, leading to synaptopathy and altered neuronal morphology.