bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis
Authors: Khosraviani Negin , Yerlici V. Talya , St-Germain Jonathan , Hou Yi Yang , Cao Shi Bo , Ghali Carla , Krishnan Rehna , Hakem Razqallah , Raught Brian , Mekhail Karim 
Published: December 2023

AbstractNucleolar ribosomal DNA (rDNA) repeats control ribosome manufacturing. rDNA harbors a ribosomal RNA (rRNA) gene and an intergenic spacer (IGS). RNA polymerase (Pol) I transcribes rRNA genes yielding the rRNA components of ribosomes. Pol II at the IGS induces rRNA production by preventing Pol I from excessively synthesizing IGS non-coding RNAs (ncRNAs) that can disrupt nucleoli. At the IGS, Pol II regulatory processes and whether Pol I function can be beneficial remain unknown. Here, we identify IGS Pol II regulators, uncovering nucleolar optimization via IGS Pol I. Compartment-enriched proximity-dependent biotin identification (compBioID) showed enrichment of the TATA-less promoter-binding TBPL1 and transcription regulator PAF1 with IGS Pol II. TBPL1 localizes to TCT motifs, driving Pol II and Pol I and maintaining its baseline ncRNA levels. PAF1 promotes Pol II elongation, preventing unscheduled R-loops that hyper-restrain IGS Pol I and its ncRNAs. PAF1 or TBPL1 deficiency disrupts nucleolar organization and rRNA biogenesis. In PAF1-deficient cells, repressing unscheduled IGS R-loops rescues nucleolar organization and rRNA production. Depleting IGS Pol I-dependent ncRNAs is sufficient to compromise nucleoli. We present the interactome of nucleolar Pol II and show its control by TBPL1 and PAF1 ensures IGS Pol I ncRNAs maintaining nucleolar structure and operation.
A rapid review of physical health risks associated with special procedures (tattooing, body piercing, acupuncture, electrolysis)
Authors: Kiseleva Meg , Csontos Judit , Edwards Deborah , Gillen Elizabeth , Mann Mala , Searchfield Lydia , Sha’aban Abubakar , Lewis Ruth , Cooper Alison , Edwards Adrian 
Published: December 2023

AbstractSpecial procedures, such as tattooing (including semi-permanent make-up), body piercing, acupuncture and electrolysis involve piercing the skin and carry a potential risk of infection and other complications. This review provides an up-to-date evidence base on the main physical health risks associated with these special procedures.Searches were conducted for research published between 2015 and 2023. Where possible, existing reviews were used. For tattooing, one systematic review including 55 studies was identified. For semi-permanent make-up, 37 cases presented in 31 case reports were identified. For body piercing, four systematic reviews were identified, which covered 174 studies. For acupuncture, one evidence map of 535 systematic reviews without age limits and one overview of 24 systematic reviews focused on children were included. For electrolysis, one case report was identified.Complications and adverse events resulting from special procedures include fungal infections, bacterial infections, viral infections, blood-borne infections, allergic reactions, malignant growths, benign growths, sarcoidosis-related reactions, and a range of other skin and eye-related adverse reactions and dental issues.Research Implications and Evidence GapsMost of the primary evidence for the physical health risks associated with the special procedures came from case reports and case series, which do not allow causal inferences or provide information on the prevalence of adverse events. Future primary research should employ more robust designs to gather evidence about the causal links between special procedures and adverse physical health events as well as about the prevalence of such events in people receiving special procedures. More evidence is needed to identify physical health risks associated with electrolysis.Policy and Practice ImplicationsThe evidence contained in this review will be used to inform the training of local authority enforcement officers and special procedures practitioners and to educate members of the public who seek to use these services. Due to the nature of the special procedures which involves piercing the skin and poses the risk of introducing infections to the body, high standards of hygiene may reduce the rate of infection.Funding statementThe Wales Centre for Evidence Based Care, and the Specialist Unit for Review Evidence, were funded for this work by the Health and Care Research Wales Evidence Centre, itself funded by Health and Care Research Wales on behalf of Welsh Government.
An integrated G-MACONT approach for healthcare supplier selection
Authors: Chakraborty Santonab , Raut Rakesh D. , Rofin T.M. , Chakraborty Shankar 
Published: December 2023

PurposeSupplier selection along with continuous evaluation of their performance is a crucial activity in healthcare supply chain management for effective utilization of scarce resources while providing quality service at an affordable price, and minimizing chances of stock-out, avoiding serious consequences on the illness or fatality of the patients. Presence of both qualitative and quantitative evaluation criteria, set of potential suppliers and participation of different stakeholders with varying interest make healthcare supplier selection a challenging task which can be effectively solved using any of the multi-criteria decision making (MCDM) methods.Design/methodology/approachTo deal with various qualitative criteria, like cost, quality, delivery performance, reliability, responsiveness and flexibility, this paper proposes integration of grey system theory with a newly developed MCDM tool, i.e. mixed aggregation by comprehensive normalization technique (MACONT) to identify the best performing supplier for pharmaceutical items in a healthcare unit from a pool of six competing alternatives based on the opinions of three healthcare professionals.FindingsWhile assessing importance of the six evaluation criteria and performance of the alternative healthcare suppliers against those criteria using grey numbers, and exploring use of three normalization procedures and two aggregation operations of MACONT method, this integrated approach singles out S5 as the most compromised healthcare supplier for the considered problem. A sensitivity analysis of its ranking performance against varying values of both balance parameters and preference parameters also validates its solution accuracy and robustness.Originality/valueThis integrated approach can thus efficiently solve healthcare supplier selection problems based on qualitative evaluation criteria in uncertain group decision making environment. It can also be deployed to deal with other decision making problems in the healthcare sector, like supplier selection for healthcare devices, performance evaluation of healthcare units, ranking of physicians etc.
Inventory and financial performance in the pharmaceutical industry: what transpired over the last two decades?
Authors: Choi Jeong Hoon , Choi Sangdo , Suresh Nallan C. 
Published: December 2023

PurposeThe objective of this study is to explore the structural attributes of the pharmaceutical industry before the onset of the COVID-19 pandemic by examining the relationship between inventory and firm performance and developing a taxonomy of pharmaceutical firms based on the earns-turns matrix.Design/methodology/approachThis study examines the inventory–firm performance linkage, considering both total inventory and its discrete inventory components in pharmaceutical firms. In addition, this research develops a new taxonomy of pharmaceutical firms based on the earns-turns matrix. A large panel dataset of firms in the US pharmaceutical industry was collected for the period 2000–2019.FindingsThe results reveal that strategic groups identified based on this taxonomy show different levels of profitability and inventory turns in the earns-turns matrix. Most pharmaceutical firms moved from the low-right to the top-left section in the earns-turns matrix, indicating that these firms have generally pursued profitability rather than effective inventory management.Research limitations/implicationsThis study explores the structural attributes of the pharmaceutical industry using the earns-turns matrix. This two-dimensional analysis may not, however, capture the full complexity of inventory–firm performance dynamics.Practical implicationsThe mapping of strategic groups on the earns-turns matrix provides a useful tool for visual representations of the dynamics of strategic groups in terms of financial performance and inventory management performance. Practitioners can use the earns-turns matrix to benchmark their firm's position against their competitors.Originality/valueThis study broadens the scope of operations management research by introducing the earns-turns matrix as an empirical validation tool for operational and strategic management theories. This study emphasizes the effectiveness of the earns-turns matrix in analyzing strategic groups of pharmaceutical firms.
Decolonising doctoral education in an era of pandemic
Authors: Manathunga Catherine 
Published: December 2023

Purpose
The purpose of this paper is to investigate the impact of the COVID-19 pandemic on doctoral education. Pandemics throughout history have generated new educational theories and practices, accelerated some trends and signalled the abrupt end of others. The unpredictable effects of the COVID-19 pandemic have particularly impacted upon First Nations and transcultural communities and People of Colour throughout the globe. A second significant recent global trend that occurred at the height of the pandemic was the reignited #BlackLivesMatter (#BLM) protest campaign. This campaign drew attention to the vast inequities faced by black, transcultural (migrant, refugee, culturally diverse and international) and Indigenous peoples and triggered rapid action in higher education institutions against racism and unconscious bias.

Design/methodology/approach
This conceptual paper draws upon postcolonial/decolonial theory to demonstrate how the COVID pandemic and #BLM movement prompts us to revitalise doctoral education.

Findings
These two issues have created renewed urgency around the need to decolonise higher education and a desire to transform the “business-as-usual” geopolitical power dynamics that continue to privilege Northern knowledge over culturally diverse knowledge systems from First Nations and transcultural contexts. A key site where special opportunities exist to effect this transformation lies in doctoral education. Doctoral education is a significant location of new knowledge creation and the development of the world’s future researchers.

Research limitations/implications
Applying post/decolonial theory enables one to rethink how doctoral education should be changed to work towards greater decolonisation.

Originality/value
This study applies Santos’ ideas about “the sociologies of emergence” in the global South to think about how doctoral education should be reconstructed as a liberated zone of decolonisation and epistemic justice.
Front-desk workforce cultural diversity and its implications for service quality in the accommodation sector: a case from Australia
Authors: Suwarto Titania , Pforr Christof , Volgger Michael 
Published: December 2023

Purpose
Language and dialect, customs and service attitudes are just a few of the characteristics that make each culture unique. Consequently, the presence of people from different cultures during a service encounter may impact service quality, as what constitutes good service quality is perceived differently across cultures. As a country with a multicultural society, culturally diverse migrants from non-English speaking backgrounds (NESB) play an increasingly important role in the Australian hospitality and accommodation sector. Within this regional context, this study aims to contribute to a more robust understanding of the influence of workforce cultural diversity on different aspects of front-desk accommodation service quality in the accommodation industry.

Design/methodology/approach
In this qualitative study, 31 semi-structured in-depth interviews with guests, front-desk staff and managers at service apartments in Western Australia were conducted, followed by validation interviews with five hospitality human resource management experts.

Findings
The results of the interviews suggest that cultural diversity of NESB staff affects several aspects of Australian accommodation front-desk services, including communication quality, staff-guest interaction and guest satisfaction. Findings point out that culturally diverse NESB front-desk staff may face cross-cultural communication barriers due to different foreign accents and the presence of local slang, improve guest-staff interaction and the overall guest experience in case of similar cultural backgrounds with guests and provide authentic representations of multiculturalism in Australia.

Originality/value
The study sheds new light on tourists’ shifting perceptions and expectations of authenticity, particularly in Australia, where cultural diversity has increased in prominence. Moreover, in the Australian accommodation service, many NESB employees who have learned American/British English have difficulty conversing with people with Australian accents, especially when Australian slang is also present.
Patient preferences for disposable and reusable vaginal specula and their willingness to compromise in the era of climate change: A cross‐sectional study
Authors: Ten Buuren Amber A. A. , Poolman Tessa B. , Bongers Marlies Y. , Bullens Lauren M. , Van Hanegem Nehalennia , Klerkx Wenche M. , Timmermans Anne , Zuithoff Nicolaas P. A. , Kwee Anneke 
Published: December 2023

AbstractObjectiveTo evaluate patient satisfaction on gynaecological examination with metal, plastic and biobased plastic vaginal specula, and to investigate whether patients are willing to compromise on comfort for a more sustainable healthcare system.DesignCross‐sectional study: population‐based survey.SettingGynaecological outpatient clinics in five Dutch hospitals.PopulationPatients during general gynaecology consultation hours.MethodsA survey containing two questions about patient demographics, four about comfort and five about sustainability and healthcare was distributed.Main outcome measuresComfort score (scale 1–10). Secondary outcomes: (1) temperature, size and ease of insertion, (2) willingness to compromise for a more sustainable healthcare system.ResultsIn all, 196 patients completed the survey. Biobased plastic vaginal specula scored significantly higher on comfort than the metal ones (mean 8.03 ± 1.65 versus 7.26 ± 1.51 respectively; P < 0.001). The biobased plastic vaginal speculum is significantly the most comfortable on temperature, whereas the metal speculum is the least comfortable (P < 0.007). Most patients are willing to compromise on comfort or are open to the reuse of disposables to contribute to a more sustainable healthcare. The majority of patients (77%) urge healthcare organisations to combat climate change.ConclusionsThere is a small but statistically significant difference in favour of a biobased plastic speculum regarding comfort score, although it might be questioned whether this is clinically relevant. Furthermore, patients are willing to compromise on comfort for a more sustainable healthcare, which should be a contributing factor in speculum selection.
Extracellular ATP inhibits excitatory synaptic input on parvalbumin positive interneurons and attenuates gamma oscillations via P2X4 receptors
Authors: Wildner Florian , Neuhäusel Tim S. , Klemz Alexander , Kovács Richard , Ulmann Lauriane , Geiger Jörg R. P. , Gerevich Zoltan 
Published: December 2023

AbstractBackground and PurposeP2X4 receptors (P2X4R) are ligand gated cation channels that are activated by extracellular ATP released by neurons and glia. The receptors are widely expressed in the brain and have fractional calcium currents comparable with NMDA receptors. Although P2X4Rs have been reported to modulate synaptic transmission and plasticity, their involvement in shaping neuronal network activity remains to be elucidated.Experimental ApproachWe investigated the effects of P2X receptors at network and synaptic level using local field potential electrophysiology, whole cell patch clamp recordings and calcium imaging in fast spiking parvalbumin positive interneurons (PVINs) in rat and mouse hippocampal slices. The stable ATP analogue ATPγS, selective antagonists and P2X4R knockout mice were used.Key ResultsThe P2XR agonist ATPγS reversibly decreased the power of gamma oscillations. This inhibition could be antagonized by the selective P2X4R antagonist PSB‐12062 and was not observed in P2X4−/− mice. The phasic excitatory inputs of CA3 PVINs were one of the main regulators of the gamma power. Associational fibre compound excitatory postsynaptic currents (cEPSCs) in CA3 PVINs were inhibited by P2X4R activation. This effect was reversible, dependent on intracellular calcium and dynamin‐dependent internalization of AMPA receptors.Conclusions and ImplicationsThe results indicate that P2X4Rs are an important source of dendritic calcium in CA3 PVINs, thereby regulating excitatory synaptic inputs onto the cells and presumably the state of gamma oscillations in the hippocampus. P2X4Rs represent an effective target to modulate hippocampal network activity in pathophysiological conditions such as Alzheimer's disease and schizophrenia.
Graft survival and postoperative complications following orthotopic renal transplantation
Authors: Territo Angelo , Selvi İsmail , Malçok Aydan , Boissier Romain , Campi Riccardo , Prudhomme Thomas , Pecoraro Alessio , Piana Alberto , Lopez‐Abad Alicia , Bañuelos Marco Beatriz , Breda Alberto , Dönmez M. İrfan 
Published: December 2023

AbstractThe iliac fossa is the most commonly used site to place the graft in renal transplantation in adults. However, iliac fossa may not be used in various conditions. Thus, orthotopic renal transplantation becomes a viable alternative for these selected patients. Given the technically challenging surgery and limited number of patients, data on the long‐term outcomes on this regard are scarce. This narrative review serves as an update on the clinical outcomes after orthotopic renal transplantation, focusing on overall recipient survival and renal graft survival, as well as postoperative complications. We found that studies to date showed a comparable survival rate in both recipients and renal grafts in the postoperative follow‐up period after orthotopic renal transplantation with a lower complication rate compared to the published data on heterotopic renal transplantation. The results of our review may encourage transplant centers to reevaluate their policies to consider orthotopic renal transplantation as an alternative technique in cases where heterotopic kidney transplantation is not possible.
Elevation filters bat, rodent and shrew communities differently by morphological traits
Authors: Monadjem Ara , Farooq Harith , Kane Adam 
Published: December 2023

AbstractAimMountains are geographic features that provide sharp elevational gradients which can accommodate a diversity of terrestrial flora and fauna. In the face of climate change, many of these species are being pushed higher to escape ever‐increasing temperatures. Despite this, we have little understanding of how species distribute themselves across mountains. African mountains in particular are less studied than mountains elsewhere and the small mammal groups that inhabit them are also poorly studied when compared to other groups. In this study, we examined the responses of three functionally distinct small mammal taxonomic groups to changes in elevation across Africa.LocationAfrica.MethodsWe calculated four functional diversity metrics for 166, 97 and 153 communities of rodents, bats and shrews comprising 225, 183 and 109 species, respectively. We employed RLQ and fourth‐corner analyses to identify any associations between the functional traits of the small mammals and elevation and other climatic variables.ResultsWe show that the species richness of the three taxa responded differently to increases in elevation, with only rodents showing a mid‐elevational hump. The composition of rodent and bat communities differed significantly in upland versus lowland sites, but bat communities showed far more overlap across the elevational gradient. Functional diversity metrics responded differently for each taxon highlighting the importance of using different taxonomic groups when studying elevational patterns of functional diversity.Main ConclusionsOur study shows that functional divergence increases with elevation in all three taxa, indicating a pervasive and broadly applicable strong environmental filtering at higher elevations. Our findings suggest that species at higher elevations may be at higher risk due to specialization. These species are losing habitat due to ongoing climate change that will force them up the elevational gradient. At the same time, the human population in Africa is predicted to triple in size by the year 2100, which will also likely contribute to further habitat loss and fragmentation. As small mammals often play essential roles in ecosystems, from seed dispersal to soil aeration and predation, understanding the susceptibility of the specialization of species at higher elevations is crucial for the effective conservation and management of mountain ecosystems, especially in light of climatic change and human expansion.
Efficacy of adipose‐derived mesenchymal stem cell therapy in the treatment of chronic micro‐ and macrovascular complications of diabetes
Authors: Mikłosz Agnieszka , Chabowski Adrian 
Published: December 2023

AbstractDiabetes mellitus is a highly prevalent disease characterized by hyperglycaemia that damages the vascular system, leading to micro‐ (retinopathy, neuropathy, nephropathy) and macrovascular diseases (cardiovascular disease). There are also secondary complications of diabetes (cardiomyopathy, erectile dysfunction or diabetic foot ulcers). Stem cell‐based therapies have become a promising tool targeting diabetes symptoms and its chronic complications. Among all stem cells, adipose‐derived mesenchymal stem cells (ADMSCs) are of great importance because of their abundance, non‐invasive isolation and no ethical limitations. Characteristics that make ADMSCs good candidates for cell‐based therapy are their wide immunomodulatory properties and paracrine activities through the secretion of an array of growth factors, chemokines, cytokines, angiogenic factors and anti‐apoptotic molecules. Besides, after transplantation, ADMSCs show great ex vivo expansion capacity and differentiation to other cell types, including insulin‐producing cells, cardiomyocytes, chondrocytes, hepatocyte‐like cells, neurons, endothelial cells, photoreceptor‐like cells, or astrocytes. Preclinical studies have shown that ADMSC‐based therapy effectively improved visual acuity, ameliorated polyneuropathy and foot ulceration, arrested the development and progression of diabetic kidney disease, or alleviated the diabetes‐induced cardiomyocyte hypertrophy. However, despite the positive results obtained in animal models, there are still several challenges that need to be overcome before the results of preclinical studies can be translated into clinical applications. To date, there are several clinical trials or ongoing trials using ADMSCs in the treatment of diabetic complications, most of them in the treatment of diabetic foot ulcers. This narrative review summarizes the most recent outcomes on the usage of ADMSCs in the treatment of long‐term complications of diabetes in both animal models and clinical trials.