bluuebunny/crossref_metadata_2025_split huggingface dataset:
First assessment of biofouling assemblages in the northern Red Sea, an important region for marine non-indigenous species transfer
Authors: Sempere-Valverde Juan , Aylagas Eva , Syomin Vitaly , Teixeira Marcos A. L. , Kolbasova Glafira D. , Ruiz-Velasco Sofía , Chebaane Sahar , Anker Arthur , Curdia João , Cadiz Ronald , Cottrell Dylan M. , Desiderato Andrea , Andrade Luiz F. , Antony Chakkiath Paul , Bocanegra-Castano Carolina , Marzucchi Matilde , Poliseno Angelo , Baker Doaa , Alabdulaziz Basmah , Torres Flor , Eweida Ameer A. , Carvalho Susana 
Published: February 2025

IntroductionMaritime traffic and coastal urbanisation significantly contribute to the introduction and proliferation of non-indigenous species (NIS). However, the lack of information might prevent effective monitoring in data-limited regions, particularly in areas experiencing demographic growth, where monitoring biofouling communities could offer crucial insights into the dynamics of NIS invasions. This study represents a baseline characterization of the biofouling communities in the northern Saudi Arabian Red Sea (NEOM region) prior to extensive urban, industrial, and commercial development.MethodsSamples were collected in November 2023 and February 2024 from seven sites within the region. At each site and time, three settlement structures, each with a PVC panel attached to a brick and rope, were sampled after 3-months deployment. Panels were analysed with photo quadrat analysis (PhQd), examined to manually collect sessile macroinvertebrate specimens for taxonomic identification using morphological and DNA barcoding analysis, and scraped for bulk DNA analyses using DNA metabarcoding. Five water samples were also collected for environmental DNA (eDNA) analysis. The environmental characteristics of each site were obtained by deploying temperature data loggers and through an environmental risk assessment.ResultsCommunity patterns observed from the PhQd and bulk DNA datasets revealed a clear separation of two sites within Sharma lagoon from the remaining sites. The eDNA analysis of seawater confirmed these spatial differences, also detecting a variation between sampling times that was not observed with the other approaches. A total of 20 NIS and 18 cryptogenic species were recorded, from which 12 were identified morphologically and the remaining detected with molecular methods only. The generally low NIS coverage confirms that the NEOM region is less affected by marine biological invasions compared to other anthropized habitats within the Red Sea. However, sites in the Sharma lagoon showed high human pressure levels and comparatively higher coverage of cryptogenic bryozoans on panels.DiscussionThis study provides the first detailed assessment of biofouling communities in NEOM, establishing a baseline and contributing to a regional species reference library for non-indigenous and cryptogenic species. As coastal development spreads, it presents both challenges and opportunities, highlighting the need for sustainable, ecosystem-based approaches to protect valuable natural areas. This baseline is essential for future monitoring of biofouling dynamics as the region develops.
Case report: dysgeusia, strawberry tongue, and psoriatic eruptions after combination treatment with adalimumab, sulfasalazine, and etoricoxib for ankylosing spondylitis
Authors: Cui Binglin , Lin Jing , Huang Yuanchun , Zhu Huachen , Zou Jinbo , Qin Jiesheng , Pan Hui , Chen Jian 
Published: February 2025

BackgroundTumor necrosis factor blockers can suppress immune system and lead to various adverse drug reactions, including deaths. We present a rare case of dysgeusia, strawberry tongue, and psoriasis-like eruptions after simultaneous administration of adalimumab, sulfasalazine, and etoricoxib.Case descriptionA 36 year-old male with ankylosing spondylitis presented with progressive loss of taste for 3 months and rashes on his upper trunk for 5 days. He had been receiving adalimumab, sulfasalazine, and etoricoxib for 9 months. After self-discontinuing the medicines, the rashes gradually subsided. On examination, swollen and red strawberry tongue were noticed. Acinetobacter pittii was isolated by sputum culture. The patient refused any treatment. During follow-up, recurrent dispersed papules/macules and desquamation appeared on his upper trunk, with erythema and erosion in umbilical region. Subsequently, generalized scalp erythema, exudates, scabs, hair bundles, redness and desquamation behind ears occurred. The tongue, taste, and skin lesions resolved sequentially and steadily until complete recovery.OutcomeHe remained in remission during 4 years follow-up. The total course of disease was around 10 months.DiscussionClinicians should be cautious of the adverse drug reactions/events due to adalimumab, sulfasalazine, and etoricoxib. Rational use of medicines is advocated.
Potential biomarkers and immune infiltration linking endometriosis with recurrent pregnancy loss based on bioinformatics and machine learning
Authors: Chen Jianhui , Li Qun , Liu Xiaofang , Lin Fang , Jing Yaling , Yang Jiayan , Zhao Lianfang 
Published: February 2025

ObjectiveEndometriosis (EMs) is a chronic inflammatory disease characterized by the presence of endometrial tissue in the non-uterine cavity, resulting in dysmenorrhea, pelvic pain, and infertility. Epidemiologic data have suggested the correlation between EMs and recurrent pregnancy loss (RPL), but the pathological mechanism is unclear. This study aims to investigate the potential biomarkers and immune infiltration in EMs and RPL, providing a basis for early detection and treatment of the two diseases.MethodsTwo RPL and six EMs transcriptomic datasets from the Gene Expression Omnibus (GEO) database were used for differential analysis via limma package, followed by weighted gene co-expression network analysis (WGCNA) for key modules screening. Protein-protein interaction (PPI) network and two machine learning algorithms were applied to identify the common core genes in both diseases. The diagnostic capabilities of the core genes were assessed by receiver operating characteristic (ROC) curves. Moreover, immune cell infiltration was estimated using CIBERSORTx, and the Cancer Genome Atlas (TCGA) database was employed to elucidate the role of key genes in endometrial carcinoma (EC).Results26 common differentially expressed genes (DEGs) were screened in both diseases, three of which were identified as common core genes (MAN2A1, PAPSS1, RIBC2) through the combination of WGCNA, PPI network, and machine learning-based feature selection. The area under the curve (AUC) values generated by the ROC indicates excellent diagnostic powers in both EMs and RPL. The key genes were found to be significantly associated with the infiltration of several immune cells. Interestingly, MAN2A1 and RIBC2 may play a predominant role in the development and prognostic stratification of EC.ConclusionWe identified three key genes linking EMs and RPL, emphasizing the heterogeneity of immune infiltration in the occurrence of both diseases. These findings may provide new mechanistic insights or therapeutic targets for further research of EMs and RPL.
The effects of lutein/ zeaxanthin (Lute-gen®) on eye health, eye strain, sleep quality, and attention in high electronic screen users: a randomized, double-blind, placebo-controlled study
Authors: Lopresti A. L. , Smith S. J. 
Published: February 2025

BackgroundLutein and zeaxanthin are fat-soluble antioxidant nutrients that have evidence of beneficial effects on vision and eye health.PurposeExamine the effects of supplementation with lutein and zeaxanthin isomers (Lute-gen®) on eye health, eye strain, sleep quality, and attention in high electronic screen users.Study designTwo-arm, 6-month, parallel-group, randomized, double-blind, placebo-controlled trial.MethodsSeventy volunteers aged 18 to 65 who used electronic screens for more than 6 h daily were supplemented with 10 mg of lutein and 2 mg of zeaxanthin-isomers or a placebo. Outcome measures included several ophthalmic examinations comprising the Schirmer tear test, photo-stress recovery time, contrast sensitivity, tear film break-up time, and self-report measures of visual fatigue, computer vision, sleep quality and attention.ResultsCompared to the placebo, lutein and zeaxanthin supplementation was associated with greater improvements in the Schirmer tear test, photo-stress recovery time, and tear film break-up time. However, there were no between-group differences in the change in self-report measures or contrast sensitivity. Lutein and zeaxanthin supplementation was well-tolerated, with no reports of serious adverse reactions or clinically significant changes in safety blood measures, including liver function, renal function, blood lipids, and full blood examination.ConclusionThe results from this study provide support for the beneficial effects of 6 months of lutein and zeaxanthin supplementation on regular users of electronic screens. Compared to the placebo, there were improvements in several ophthalmic examinations for dry eyes and visual health. However, these findings were not corroborated by group differences in the administered self-report measures. Lutein and zeaxanthin were well tolerated, with no serious adverse effects or significant changes in vital signs or blood safety measures.
Determinants of adherence to the Mediterranean diet among individuals with type 2 diabetes mellitus living in Mediterranean countries: a systematic review
Authors: Ayoub Janot J. , Haidar Suzan A. , Blaak Ellen E. , De Vries Nanne K. 
Published: February 2025

BackgroundPatients with type 2 diabetes mellitus (T2DM) are often encouraged to adopt a healthy diet, such as the Mediterranean Diet (MD) yet limited evidence exists about adherence. An increased shift toward a “Western” dietary pattern was observed.ObjectiveThis systematic review aims to gain insight into the various factors that may enhance or reduce adherence to the MD in patients with T2DM residing in Mediterranean countries.MethodWe retrieved published studies from 2000 to 2023 from PubMed, Cochrane, Embase, CINAHL, Web of Science, Medline, and PsycINFO databases. The criteria for inclusion included individuals residing in Mediterranean countries aged 18+ with T2DM who underwent assessment using a validated MD scoring tool. We included 17 studies, with participant numbers ranging from 106 to 7,447.ResultsCompliance with the MD was moderate, with the most significant determinants of adherence being age, physical activity, body mass index (BMI), marital status, and educational level. However, limited information is available on psychological and economic determinants.ConclusionVarious factors, especially sociodemographic ones, influence adherence to the MD among patients with T2DM. Future research should explore economic and psychological factors that may significantly impact adherence.Systematic review registerProspero: CRD42023396094.
Case report: Chest radiotherapy-induced vertebral fractures in lung cancer patients: a case series and literature review
Authors: Arrieta Oscar , Lozano-Ruiz Francisco , Guijosa Alberto , Soberanis-Pina Pamela , Blake-Cerda Monika , Gómez-García Ana Pamela , Maldonado-Magos Federico , Conde-Flores Emilio , Cardona Andrés F. , Pérez Álvarez Sandra Ileana , Cabrera-Miranda Luis Antonio 
Published: February 2025

BackgroundAs survival rates for lung cancer (LC) patients continue to rise, the adverse impacts of therapies become more relevant. Radiotherapy is known to negatively affect bone health. However, radiotherapy-induced vertebral fractures in lung cancer patients remain an exceedingly rare and underrecognized condition that could be mistaken for bone metastasis.Case presentationWe identified three LC patients (all long-term survivors), aged 67 to 81, who developed thoracic vertebral fractures post-chest radiotherapy, within irradiated fields; two had advanced non-small cell lung cancer (NSCLC) and one had extensive small cell lung cancer (SCLC). Baseline imaging confirmed that the fractures occurred after therapy. The median time from radiotherapy to fracture onset was 19 months (range: 1-30 months), with a median follow-up time from the initial fracture of 39 months (range: 37-61 months). All observed fractures were compressive in nature. These patients shared common characteristics, including advanced age, a history of heavy smoking, and high radiation doses. Additionally, hypermetabolic activity at the fracture sites necessitated MRI to differentiate these fractures from bone metastases. Management involved interventional strategies such as vertebroplasty, kyphoplasty, and rhizotomy, along with general and pharmacological measures to prevent subsequent fractures.ConclusionsDespite their low incidence, radiotherapy-induced vertebral fractures in LC patients are clinically significant and may resemble bone metastases on PET-CT imaging. MRI, alongside risk factors similar to those of osteoporosis, can facilitate prompt identification and differentiation. As survival rates in LC patients improve, the relevance of this adverse effect increases, underscoring the need for implementing bone protective strategies to further enhance patient outcomes and quality of life.
Assessment of myocardial deformation by CMR tissue tracking reveals left ventricular subclinical myocardial dysfunction in patients with gynecologic cancer undergoing chemotherapy
Authors: Tao Kai , Ye Lu , Xu Yan-Jia-Ni , Yang Meng-Xi , Yin Ru-Tie , Li Qing-Li , Lin Xiao-Juan , Li Ke-Min , Song Liang , Ma Yu , Zhong Lan , Hu Ying , Xu Hua-Yan , Wang Dan-Qing , Guo Ying-Kun 
Published: February 2025

BackgroundChemotherapy-induced cardiotoxicity is a concern for patients with gynecologic cancer. This study aimed to assess left ventricular (LV) myocardial deformation in patients with gynecologic cancer undergoing chemotherapy and to investigate the association between myocardial deformation and chemotherapy factors.MethodsCardiac magnetic resonance (CMR) was performed to assess LV deformation parameters using CMR tissue tracking based on cine images. Serum myocardial injury biomarker were measured. Deformation parameters were compared between healthy controls and patients. Changes in deformation were assessed as chemotherapy progressed. Correlations between LV deformation parameters, clinical characteristics, and serum myocardial injury biomarkers were also analyzed.ResultsA total of 86 patients with gynecologic cancer and 30 normal controls were included. Among the patients, 41 completed CMR follow-up with a median interval of 6 months. Compared to the controls, patients exhibited lower absolute value of global radial strain (GRS) (37.30 ± 8.94% vs. 44.32 ± 8.44%), global circumferential strain (GCS) (-22.12 ± 3.05% vs. -24.08 ± 2.13%) and global longitudinal strain (GLS) (median -15.72% [IQR-17.13 to -13.58%] vs. -17.51 ± 2.00 %) (all p &lt; 0.05). Patients with preserved LV ejection fraction (LVEF) also showed impaired global strain (all p &lt; 0.05). GRS (39.71 ± 8.09% vs. median 30.56% [IQR 26.52 to 38.15%]; p = 0.001), GCS (-23.45 ± 2.09% vs. median -19.71% [IQR -21.71 to -19.10%]; p &lt; 0.001) and GLS (-16.17 ± 2.42% vs. median -12.12% [IQR -14.10 to -8.53%]; p&lt; 0.001) further decreased as the number of chemotherapy cycles increased during follow-up (all p &lt; 0.05). Multivariate analysis showed that GCS was independently associated with the number of chemotherapy regimens (Standard regression coefficient [β] = 0.397, p &lt; 0.001).ConclusionsMyocardial deformation is more sensitive than LVEF in detecting subclinical left ventricular dysfunction in patients with gynecologic cancer undergoing chemotherapy. GCS was associated with the number of chemotherapy regimens.
Overview of female healthcare providers’ outlooks on cervical cancer screening: a narrative review
Authors: Gezimu Wubishet , Demeke Ababo , Bekele Firomsa , Alemu Solomon Seyife , Fikadu Lema , Demsash Addisalem Workie , Dube Geleta Nenko , Kitil Gemeda Wakgari , Ezo Elias 
Published: February 2025

BackgroundCervical cancer is the second most frequent gynecologic cancer. Uniquely, it is easily preventable and treatable cancer if identified early. The insights of healthcare providers about cervical cancer screening have a crucial role in prevention and treatment. However, there has been limited literature on the providers’ perspectives on cervical cancer screening.ObjectiveThis review narrated the female healthcare providers’ (FHCPs’) outlooks on cervical cancer screening in terms of risk perceptions, awareness, knowledge, attitude, practice, and possible barriers.MethodsA thorough literature search was conducted to identify studies conducted on female healthcare providers’ overview of the perceived risk of cervical cancer, cervical cancer screening awareness, knowledge, attitude, and practice, as well as barriers to cervical cancer screening. Databases such as PubMed, Medline, Embase, Virtual Health Library, and Google Scholar were used to search for articles.ResultsAccordingly, this review identified that female healthcare providers have a low perceived risk of the disease, poor awareness and knowledge, unfavorable attitudes, and low uptake of screening practices. Furthermore, this review highlights the obstacles to cervical cancer screening acceptance, such as service inaccessibility, a lack of training and education, and fear of the procedure and results.ConclusionThis narrative review described the variable distribution of the FHCPs’ perceived risk of acquiring cervical cancer (CC). Poor knowledge and screening practices were observed. Moreover, the barriers to cervical cancer screening uptake were described. Given that healthcare providers are on the frontlines (act as role models) in increasing the community’s cervical cancer screening uptake, we suggest concerned bodies increase screening access and implement staff training programs. In addition, further mixed studies should be considered to deeply understand the possible attributes ingrained in individual and social belief systems.
Comparative efficacy of topical commercial Chinese polyherbal preparation for vulvovaginal candidiasis: a network meta-analysis
Authors: Wu Lizheng , Jing Shangwen , Li Na , Cao Dandan , Pei Fangli , Luo Yantong , Chen Xiaoxin , Huang Yingjie , Zeng Cheng 
Published: February 2025

ObjectiveTo systematically evaluate the differences in the efficacy of commonly used topical commercial Chinese polyherbal preparations (CCPPs) for vulvovaginal candidiasis (VVC), and to provide evidence-based reference for clinical drug regimens.MethodsComputer searched major Chinese and English literature databases, and collected randomized controlled trials (RCTs) of external use of CCPPs combined with conventional treatment (CT, test group) versus CT alone (control group) in patients with VVC. After screening the literature, the quality of the included studies was assessed using the risk of bias assessment tool recommended in 5.3 of the Cochrane Handbook. Outcome data for each outcome measure were extracted and a network meta-analysis was performed using Stata 15.0.ResultsA total of 74 RCTs with a total of 8,151 patients were included, nine interventions were involved. The results of network meta-analysis showed that, Fufang Shajiziyou suppository combined with CT was more effective in improving the negative conversion rate and controlling the recurrence rate of Candida albicans. Kangfu gel combined with CT had the better effect in relieving pruritus. Baofukang suppository combined with CT was the most effective in reducing leukorrhea. Fufukang spray combined with CT had fewer adverse reactions.ConclusionThe addition of CCPPs to CT may acquire a better curative effect in patients with VVC and external does not significantly increase the occurrence of adverse reactions. However, due to the low quality of the included literature, larger-scale, higher-quality clinical studies are still needed.Systematic Review RegistrationPROSPERO, NO: CRD42023410244.
ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway
Authors: Hou Jing-Yu , Wu Hao , Li Shuang-Mei , Li Xiao-Jing , Yang Shu-Jun , Chen Xu-Xiang , Zhou Chang-Qing , Long Hui-Bao , Wu Hai-Dong , Fu Jia-Ying , Guo Ya-Jie , Wang Tong 
Published: February 2025

BackgroundBone marrow mesenchymal stem cells (BMSCs) hold promise for repairing myocardial injury following acute myocardial infarction (AMI), but their clinical application is hindered by poor migration, homing efficiency, and survival rates. Previously, we demonstrated that ELABELA (ELA), a small peptide, enhances the survival of rat BMSCs under hypoxia-reoxygenation (H/R) conditions by activating ERK1/2. However, the role of ELA in promoting BMSCs migration and homing to injured cardiomyocytes remains unclear.MethodsPrimary BMSCs and neonatal rat ventricular myocytes (NRVMs) were isolated and cultured. NRVMs were exposed to H/R to mimic the microenvironment of AMI in vitro. The migration of BMSCs toward the injured myocardium was assessed in different treatment groups using transwell and chemotaxis assays. Additionally, in vivo studies were performed using a rat myocardial infarction/reperfusion injury (MI/RI) model with DIR-labeled BMSCs. Cardiac repair was evaluated through fluorescence imaging, echocardiography, and histological analysis. Transcriptome sequencing and bioinformatics analysis were employed to identify and validate the mechanisms by which ELA promoted the migration of BMSCs. A dual luciferase assay was used to investigate the interaction between Exo70 and miR-299a-5p. Subsequently, a series of experimental procedures were performed, including sequential silencing of APJ or Exo70, overexpression of miR-299a-5p, inhibition of ERK1/2 phosphorylation, assessment of BMSCs migration through transwell and scratch assays, detection of F-actin polymerization via immunofluorescence, and evaluation of the expression levels of each factor using qPCR and Western blotting.ResultsIn vitro, the migration ability of ELA-pretreated BMSCs was significantly augmented in the H/R environment. ELA pretreatment effectively heightened the homing capacity of BMSCs to the site of myocardial injury and their proficiency in repairing myocardial damage in vivo. Transcriptome sequencing revealed upregulation of Exo70 in ELA pretreated BMSCs, which promoted F-actin polymerization and migration. Overexpression of miR-299a-5p reduced Exo70 expression and impaired BMSCs migration. ELA also activated ERK1/2 phosphorylation, while inhibition of ERK1/2·with U0126 abrogated F-actin polymerization and migration, increasing miR-299a-5p levels and reducing Exo70.ConclusionELA enhances BMSCs migration and homing to injured cardiomyocytes by activating the APJ receptor, promoting ERK1/2 phosphorylation, downregulating miR-299a-5p, and upregulating Exo70, providing a potential therapeutic strategy for improving stem cell-based cardiac repair.