bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of education on knowledge, attitude and practices of safety precautions among laboratory technicians of a tertiary care center in Mangalore
Authors: B. V. Mamatha , M.Bhat Bhagyajyothi , Shreya Crasta Preeval , P. Khadilkar Niranjan 
Published: January 2024

Introduction and Aim: The clinical laboratory technicians are expected to have knowledge, to have a favourable attitude and practice safety precautions at their workplace. Though they are trained with safety precautions during their study period, they may not adhere to them upon joining the job. The present study was aimed at assessing the effect of educational intervention on knowledge, attitude and practices of safety precautions among laboratory technicians of our medical college hospital.

Materials and Methods: This interventional study included all the technicians. A validated questionnaire containing questions related to personal information, knowledge, attitude and practices of safety precautions was used. Pre-test was conducted followed by a lecture session regarding safety precautions. After one hour, post-test was conducted in a similar way as pre-test and responses were collected. Data was analysed using the statistical software SPSS version 28. Qualitative variables were presented as frequency and percentage and quantitative variables as mean SD. McNemar test was employed to find the association between the knowledge, attitude questions with pre-test and post-test scores. Paired t test was used for comparison of knowledge and attitude overall scores. p value less than 0.05 was considered statistically significant.

Results: The study showed a significant difference at the level of knowledge towards safety precautions before and after the educational intervention. There was no significant difference at the levels of attitude among the participants.

Conclusion: Regular training of safety precautions may improve the knowledge of laboratory technicians, which will benefit both the technicians and the institution.
Detection of the effect of synthetic siRNA on efflux pump MexA gene expression and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa
Authors: M. Al-Qurashi Farah , Abbas Al-Draghi Wathiq 
Published: January 2024

Introduction and Aim: Pseudomonas aeruginosa is a nosocomial infection with an ability to develop high levels of antibiotic resistance. The efflux pump system is one of the mechanisms that is linked to multidrug resistance in P. aeruginosa. In this study, we employed siRNA loaded on gold nanoparticles against the MexA efflux pump gene to decrease the MexA gene expression in P. aeruginosa and estimated antibiotic resistance after gene silencing.

Materials and Methods: This study examined four strains of P. aeruginosa isolated from patients in various hospitals in Baghdad. Bacteria isolated were identified by biochemical tests and Vitek compact 2 system. Single-stranded siRNA (33bp) designed in this study was loaded onto gold nanoparticles (AuNPls). Detection of the MexA gene was carried out by conventional PCR technique. The expression of MexA gene was examined by qRT-PCR in order to determine if the siRNA have impacted on MexA gene expression and on the antibiotic resistance in

aeruginosa

Results: This study showed that the mRNA expression level of the MexA gene exhibited a decrease in fold change CT -2 in P. aeruginosa (isolates numbers 67, 66,49, and PDR(5p)) when examined in vitro. The specific fold change values observed were (0.202, 0.040, 0.063, and 0.163) respectively. The resistance percentages of antibiotics tested was observed to increase after MexA gene silencing.

Conclusion: Targeting the MexA gene with synthetic siRNA may be a unique approach to diminish P. aeruginosa resistance to antibiotics. However, many unexpected consequences may occur when utilizing any genetic manipulation in bacteria.
Safety and efficacy of a combined treatment with corneal collagen cross-linking and lamellar keratoplasty in patients with keratitis
Authors: Turgunbaev Nurlan , Umetaliev Yusupzhan , Gogaeva Laila , Ostroverkhov Aleksandr , Yrysov Keneshbek 
Published: January 2024

Introduction and Aim: There is a link between an increase in eye infections, environmental deterioration, increased intensity of eye surgery, and the diversification of diagnostic techniques and the development of corneal disease and eye injuries. This study aims to assess the safety and efficacy of a complex treatment for corneal ulcers (keratitis) that combines corneal collagen cross-linking (CXL) with lamellar keratoplasty (LK).

Materials and Methods: This study consisted of 28 eyes from 28 patients with keratitis. All patients were divided into the main and control groups; each group had 14 patients (14 eyes). All patients underwent a standard ophthalmological examination, which included visometry without correction, with correction, tonometry, biomicroscopy, and ophthalmoscopy.

Results: High postoperative astigmatism was found in 50% of cases (7 eyes) of the control group and in 21.4% of cases (3 eyes) of the main group over the 1-6 months follow-up period. Both groups visual acuity development dynamics were very similar from the three-month to three-year follow-up period, and the average visual acuity with maximal correction was between 0.4 - 0.5.

Conclusion: Ultraviolet radiation exposure during CXL increases the graft's strength, the recipient's corneal tissue in the suture area, and the pathological flora's direct bactericidal and virucidal effects, reducing infectious process recurrences.
Increased circulatory plasma and urinary exosomal microRNA-191a as a prognostic biomarker in patients with nephrotic syndrome
Authors: Usman Bedrabettu Rahinaz , Pai B. H. Santhosh , Rao C. K. Anuradha 
Published: January 2024

Introduction and Aim: Nephrotic syndrome is one of the most common problems in kidney disease. Renal biopsy is the only standard method that determines the renal cells scarring and which also classifies the pathology of the disease. It is one of the invasive procedures with its own potential complications. So generally, it will not be suitable for frequent monitoring. MicroRNAs (miRNAs), are the short noncoding RNA molecules that regulate gene expression and are conventionally constituted of 22–25 nucleotides. MicroRNAs by binding to messenger RNAs suppress the gene expressions and it regulates different path physiological processes. In renal tissue, miRNAs also regulate the tubular and glomerular damage and proteinuria. MicroRNA has the major role in various kidney diseases due to its functional role in apoptosis, cell proliferation, differentiation, and development Elevated levels of plasma and urinary microRNAs can predict a high risk of renal function loss, disease progression and histological diagnosis. miRNA plays a very significant role in disease monitoring as well. Thus, the miRNAs have potential which could be developed as biomarkers in diagnosed cases of nephrotic syndrome. The present study was conducted to find out the specific role of miRNA-191a in nephrotic syndrome.

Materials and Methods: Quantitative real-time PCR was used to detect the overexpressed plasma miRNA by Taqman microarray. U6sn RNA were selected as a control. Using a primer of miRNA191a, quantification of mi RNA-191a was done by RT-qPCR.

Results: We found significant difference in f mol concentration among nephrotic syndrome at diagnosis, nephrotic syndrome after follow-up of three months and in the control (one-way ANOVA F=1321).

Conclusion: Increased miRNAs in patients with nephrotic syndrome can be used as the potential biomarker for monitoring the diagnosed disease.
Exploring Mycobacterium tuberculosis microarray data: Identification of interleukin (IL) as a key deregulated gene family for targeted therapy with phytochemicals
Authors: Sayyed Shamabanu , A.S. Sriranjini , Selvakumar Kamalnath , L.S. Hallur Raghavendra 
Published: January 2024

Introduction and Aim: The contemporary global health landscape is shadowed by the persistent menace of Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), a recalcitrant infectious malady. Despite medical advances, TB remains a formidable health threat, necessitating innovative strategies. Emerging drug-resistant strains further complicate its management, compelling the quest for novel biomarkers and therapeutic targets. In this context, biomarker identification assumes salience for enhanced TB management.

Methods: The Microarray technology, with its multifaceted gene expression analysis capability, gains prominence in this endeavor. Employing microarray datasets, this study identified perturbed gene families, discerning nuanced TB pathogenesis and drug resistance through network pharmacology. In-silico computational techniques including molecular docking, molecular dynamic simulation and pharmacokinetics were employed to attain a comprehensive understanding of pharmacological attributes of phytocompounds towards interleukins.

Results: Intriguingly, the representatives of the IL family were overrepresented and highly deregulated. To address TB's therapeutic conundrum, the study synthesizes microarray insights with phytochemical potential. Strikingly, IL-6 manifested as a key player, engaging in substantial protein-protein interactions within the network. Molecular docking unveiled Quercetin, Apigenin, Luteolin, Eupatilin, and Jaceidin as potent IL-6 interactors, with Quercetin showcasing notable binding affinity.
.
Conclusion: Quercetin, an anti-inflammatory agent, targeting IL-6 might mitigate detrimental immune responses and tissue damage, aiding TB management. Further exploration involves in vitro and in vivo validation of Quercetin-IL-6 interactions, assessing their impact on TB immune responses and pathogenesis. This study pioneers’ potential interventions, merging gene insights with phytochemical prowess, towards innovative TB management.
SNP IFNG rs1861494 gene polymorphism in pediatric patients with gastroenteritis and its relation to co-infectious with Adenovirus and Cryptosporidium
Authors: Hashim Abbas Fatima , S. Ibrahim Maryam , Hammad Mohammed Al-Alwany Shakir , A. Fatal Ghassan , Hasan Mohammed Ali Saad , Fadhil Bounya Sara 
Published: January 2024

Introduction and Aim: The most common cause of infantile gastroenteritis is enteric adenoviruses, while Cryptosporidiosis is caused by Cryptosporidium. The function of IFN-gamma (IFNG/IFN-y) in immunological responses, inflammation, and autoimmune disease is increasingly recognized. This study aimed to examine intestinal co-infections of Cryptosporidium and adenovirus in pediatrics and the role of IFNG rs1861494 polymorphism to gastroenteritis among Iraqi children.
Materials and Methods: This case control study included 75 pediatric patients with severe gastroenteritis, aged 3-120 months, with a mean age of 30.64 months. The apparently healthy control (AHC) in this study included 25 pediatric individuals with a mean age of 27.64 months. Total genomic DNA extracted from stool specimens were subjected to PCR detection of human adenovirus virus. SNP IFNG rs1861494 polymorphism was detected using the ARMS-PCR technique.
Results: The percentage of males to female ratio in this study was 54:46. The positive PCR result of HADV7 was 28% (14 of 50 cases) while the positive result of Cryptosporidium was 6% (6 of 75 cases).Genotyping studies showed the GG genotype increased at rate OR=2.67 as compared to AG and AA genotypes for the SNP IFNG rs1861494. The SNP IFNG rs1861494 results in gastroenteritis were highly correlated with HADV7 and Cryptosporidium (respectively: r = 0.968, P = 0.007 as well as r = 0.984, P = 0.008).
Conclusions: Adenovirus diarrhea in Iraqi pediatric patients is associated with SNP rs1861494 polymorphism and could possibly be used as a biomarker in assessing the susceptibility of Iraqi children to the virus.
HUBUNGAN PEMENUHAN GIZI DAN TINGKAT KECEMASAN DENGAN PENGELUARAN ASI PADA IBU POST PARTUM DI KOTA PADANGSIDIMPUAN TAHUN 2023
Authors: Pebrianthy Lola , Saragih Sarli 
Published: January 2024

Ibu menyusui memerlukan gizi yang cukup untuk kesehatan tubuh dan bayinya. Gangguan psikologi pada ibu menyebabkan berkurangnya pengeluaran ASI. Penelitian ini bertujuan Untuk mengetahui hubungan pemenuhan gizi dan tingkat kecemasan dengan pengeluaran ASI pada ibu post partum di Kota Padangsidimpuan Tahun 2023. Jenis penelitian ini menggunakan desain Crossecsional study. Populasi dalam penelitian ini adalah ibu post partum yang memberikan ASI kepada bayinya yaitu sebanyak 30 orang di Kota Padangsidimpuan dengan jumlah sampel adalah 30 orang. Teknik pengambilan sampel adalah total sampling. Hasil penelitian diperoleh bahwa ada hubungan antara pemenuhan gizi (p value = 0,025) dan tingkat kecemasan (p value = 0,001) dengan pengeluaran ASI pada ibu post partum di Kota Padangsidimpuan tahun 2023. Kesimpulan diperoleh bahwa ada hubungan pemenuhan gizi dan tingkat kecemasan dengan pengeluaran ASI pada ibu post partum. Diharapkan kepada ibu agar menambah pengetahuan mengenai pemenuhan gizi pada ibu post partum agar ibu dapat menghasilkan ASI yang banyak serta menjaga kondisi psikolgi yang baik agar tidak terjadi stres yang berlebihan.
Formulasi Ekstrak Daun Sirih Hijau ( Piper Betle L) Sebagai Anti Jerawat
Authors: Harahap Cory , Yanti Susi , Nova Ira , Natunna Sofiyah 
Published: January 2024

Abstrak
Daun sirih hijau (Piper betle L.) mengandung flavanoid yang cukup baik dalam menghambat pertumbuhan bakteri penyebab jerawat, salah satunya Propionibacterium acnes. Tujuan penelitian ini adalah untuk mengetahui bedak tabur anti jerawat ekstrak daun sirih hijau (Piper betle L.) dapat menghabat pertumbuhan bakteri Propionibacterium acnes, dan untuk mengetahui ekstrak daun sirih hijau (Piper betle L.) dapat diformulasikan menjadi sediaan bedak tabur anti jerawat. Menggunakan metode eksperimental. Hasil penelitian pada uji skrining fitokimia positif mengandung flavanoid, tanin, saponin. Uji organoleptis berwarna hijau dan berbau khas daun sirih hijau. Uji homogenitas sediaan homogen. Uji pH 5,22. Uji daya hambat antibakteri 6,5-10 mm termasuk dalam kategori daya hambat sedang, dimana kategori daya hambat sedang yaitu sebesar 5-10 mm. uji iritasi tidak terdapat reaksi yang menunjukkan adanya iritasi pada responden. Uji hedonik pada sediaan bedak tabur yang paling disukai oleh responden formula sediaan 3 konsenterasi 6%. Kesimpulan dari penelitian ini adalah bedak tabur anti jerawat ekstrak daun sirih hijau (Piper betle L.) dapat menghambat pertumbuhan dari bakteri Propionibacterium acnes dan daun sirih hijau (Piper betle L.) dapat diformulasikan menjadi bedak tabur anti jerawat.

Katakunci:Daun sirih hijau, Jerawat, Bedak tabur

LOOSE POWDER FORMULATION ANTI-ACNE EXTRACT
GREEN SRIH LEAVES (Piper betle L.)

Abstract
Green betel leaf (Piper betle L.) contains flavanoids that are quite good at inhibiting the growth of acne-causing bacteria, one of which is Propionibacterium acnes. The purpose of this study was to determine the anti-acne powder of green betel leaf extract (Piper betle L.) can inhibit the growth of Propionibacterium acnes bacteria, and to determine the green betel leaf extract (Piper betle L.) can be formulated into an anti-acne powder preparation. Using experimental method. The results of the research on phytochemical screening test were positive for flavanoids, tannins, saponins. Organoleptical test is green and smells typical of green betel leaves. Homogeneity test is homogeneous preparation. pH test 5.22. The antibacterial inhibition test of 6.5-10 mm is included in the category of moderate inhibition, where the category of moderate inhibition is 5-10 mm. irritation test there is no reaction that indicates irritation to the respondent. Hedonic test on the preparation of loose powder which is most preferred by respondents in the preparation formula 3 with a concentration of 6%. The conclusion of this study is that anti-acne powder of green betel leaf extract (Piper betle L.) can inhibit the growth of Propionibacterium acnes bacteria and green betel leaf (Piper betle L.) can be formulated into anti-acne powder.

Keywords: Green betel leaf, Acne, Loose powder
PENGARUH BABY SPA TERHADAP PERKEMBANGAN KEMAMPUAN MOTORIK BAYI USIA 3-6 BULAN DI DESA TELAGA SARI TAHUN 2023
Authors: Susanti Novi , Sinaga Meiyana , Silalahi Efi Satriana 
Published: January 2024

Perkembangan motorik merupakan sesuatu proses kemasakan atau gerak yang langsung melibatkan otot-otot untuk bergerak dan proses pensyarafan yang menjadi seseorang mampu menggerakkan tubuhnya. WHO menyatakan, bahwa 5-10% anak –anak usia prasekolah mengalami disfungsi otak minor, termasuk gangguan perkembangan motorik halus. Indonesia anak mengalami keterlambatan perkembangan umum belum diketahui dengan pasti , tetapi diperkirakan sekitar 1-3% anak di bawah usia 5 tahun mengalami keterlambatan perkembangan umum. Salah satu cara yang dapat digunakan untuk memenuhi kebutuhan pada perkembangan motorik adalah dengan melakukan baby spa. Tujuan penelitian ini untuk melihat bahwa treatment yang dilakukan dapat meningkatkan Perkembangan Motorik Bayi Usia 3-6 Bulan di Desa Medan Krio Tahun 2023.
Jenis desain penelitian ini menggunakan pre-Eksperimen Design dengan rancangan One Group Pretest-Postest Design. Populasi dalam penelitian ini yaitu bayi dengan usia 3-6 bulan di desa medan krio dengan teknik pengambilan sampel secara Perposivo Sampling yang terdiri dari 30 responden.
Hasil penelitian menunjukkan perkembangan bayi sesudah dilakukan baby spa lebih tinggi dari pada sebelum baby spa. Setelah mendapat perlakukan baby spa terjadi peningkatan bayi dalam kategori caution dari 2 menjadi 16 (53.3) bayi, kategori normal dari 13 menjadi sebanyak 14 (46.7) bayi dan dalam kategori caution telah terjadi peningkatan menjadi kategori advance dengan nilai p= 015b (<0,05). Sehingga dapat disimpulkan bahwa Hipotesa diterima berarti Ada Pengaruh Baby Spa Terhadap Perkembangan Kemampuan Motorik Bayi Usia 3-6 Bulan Di Desa Medan Krio Tahun 2023.
Kesimpulan dalam penelitian ini yaitu Ada Pengaruh Baby Spa Terhadap Perkembangan Kemampuan Motorik Bayi Usia 3-6 Bulan Di Desa Medan Krio Tahun 2023.Disarankan kepada tenaga kesehatan untuk mengembangkan promosi dan edukasi tentang baby spa kepada masyarakat khususnya pada orang tua untuk meningkatkan perkembangan motorik bayi.
The contribution of residential wood combustion to the PM2.5 concentrations in the Helsinki metropolitan area
Authors: Kangas Leena , Kukkonen Jaakko , Kauhaniemi Mari , Riikonen Kari , Sofiev Mikhail , Kousa Anu , Niemi Jarkko V. , Karppinen Ari 
Published: January 2024

Abstract. This article has investigated the contribution of residential wood combustion (RWC) to the fine particulate matter (PM2.5) concentrations in the Helsinki metropolitan area (HMA) for 6 years, from 2009 to 2014. We have used the PM2.5 concentrations measured at eight air quality measurement stations. The dispersion of emissions on an urban scale was evaluated with multiple-source Gaussian dispersion models, UDM-FMI (urban dispersion model of the Finnish Meteorological Institute) and CAR-FMI (Contaminants in the Air from a Road model of the Finnish Meteorological Institute), and on a regional scale using the chemical transport model SILAM (System for Integrated modeLling of Atmospheric coMposition). The overall agreement of the predicted concentrations with measurements of PM2.5 was good or fairly good for all stations and years; e.g. at the permanent residential station the daily average values of the index of agreement ranged from 0.69 to 0.81, and the fractional bias values ranged from −0.08 to 0.11, for the considered 6 years. Both the measured and predicted daily averaged concentrations showed increasing trends towards the lower-temperature values. The highest predicted annual averaged concentrations in the region occurred in the vicinity of major roads and streets and in the suburban residential areas to the northwest, north, and northeast of the city centre. The average concentrations of PM2.5 attributed to RWC in winter were up to 10- or 15-fold, compared to the corresponding concentrations in summer. During the considered 6-yearly period, the spatially highest predicted fractions of RWC of the annual PM2.5 concentrations ranged from 12 % to 14 %. In winter, the corresponding contributions ranged from 16 % to 21 %. The RWC contribution was higher than the corresponding urban vehicular traffic contribution at all the residential stations during all years. The study has highlighted new research needs for the future, in particular (i) the modelling of the RWC emissions that would be explicitly based on the actual ambient temperatures and (ii) the modelling of the impacts of the most important holiday periods on the emissions from RWC.
Identifying and correcting interferences to PTR-ToF-MS measurements of isoprene and other urban volatile organic compounds
Authors: Coggon Matthew M. , Stockwell Chelsea E. , Claflin Megan S. , Pfannerstill Eva Y. , Xu Lu , Gilman Jessica B. , Marcantonio Julia , Cao Cong , Bates Kelvin , Gkatzelis Georgios I. , Lamplugh Aaron , Katz Erin F. , Arata Caleb , Apel Eric C. , Hornbrook Rebecca S. , Piel Felix , Majluf Francesca , Blake Donald R. , Wisthaler Armin , Canagaratna Manjula , Lerner Brian M. , Goldstein Allen H. , Mak John E. , Warneke Carsten 
Published: January 2024

Abstract. Proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) is a technique commonly used to measure ambient volatile organic compounds (VOCs) in urban, rural, and remote environments. PTR-ToF-MS is known to produce artifacts from ion fragmentation, which complicates the interpretation and quantification of key atmospheric VOCs. This study evaluates the extent to which fragmentation and other ionization processes impact urban measurements of the PTR-ToF-MS ions typically assigned to isoprene (m/z 69, C5H8H+), acetaldehyde (m/z 45, CH3CHO+), and benzene (m/z 79, C6H6H+). Interferences from fragmentation are identified using gas chromatography (GC) pre-separation, and the impact of these interferences is quantified using ground-based and airborne measurements in a number of US cities, including Las Vegas, Los Angeles, New York City, and Detroit. In urban regions with low biogenic isoprene emissions (e.g., Las Vegas), fragmentation from higher-carbon aldehydes and cycloalkanes emitted from anthropogenic sources may contribute to m/z 69 by as much as 50 % during the day, while the majority of the signal at m/z 69 is attributed to fragmentation during the night. Interferences are a higher fraction of m/z 69 during airborne studies, which likely results from differences in the reactivity between isoprene and the interfering species along with the subsequent changes to the VOC mixture at higher altitudes. For other PTR masses, including m/z 45 and m/z 79, interferences are observed due to fragmentation and O2+ ionization of VOCs typically used in solvents, which are becoming a more important source of anthropogenic VOCs in urban areas. We present methods to correct these interferences, which provide better agreement with GC measurements of isomer-specific molecules. These observations show the utility of deploying GC pre-separation for the interpretation PTR-ToF-MS spectra.