bluuebunny/crossref_metadata_2025_split huggingface dataset:
An extended Tudor domain within Vreteno interconnects Gtsf1L and Ago3 for piRNA biogenesis in Bombyx mori
Authors: Bronkhorst Alfred W , Lee Chop Y , Möckel Martin M , Ruegenberg Sabine , de Jesus Domingues Antonio M , Sadouki Shéraz , Piccinno Rossana , Sumiyoshi Tetsutaro , Siomi Mikiko C , Stelzl Lukas , Luck Katja , Ketting René F 
Published: November 2023

AbstractPiwi‐interacting RNAs (piRNAs) direct PIWI proteins to transposons to silence them, thereby preserving genome integrity and fertility. The piRNA population can be expanded in the ping‐pong amplification loop. Within this process, piRNA‐associated PIWI proteins (piRISC) enter a membraneless organelle called nuage to cleave their target RNA, which is stimulated by Gtsf proteins. The resulting cleavage product gets loaded into an empty PIWI protein to form a new piRISC complex. However, for piRNA amplification to occur, the new RNA substrates, Gtsf‐piRISC, and empty PIWI proteins have to be in physical proximity. In this study, we show that in silkworm cells, the Gtsf1 homolog BmGtsf1L binds to piRNA‐loaded BmAgo3 and localizes to granules positive for BmAgo3 and BmVreteno. Biochemical assays further revealed that conserved residues within the unstructured tail of BmGtsf1L directly interact with BmVreteno. Using a combination of AlphaFold modeling, atomistic molecular dynamics simulations, and in vitro assays, we identified a novel binding interface on the BmVreteno‐eTudor domain, which is required for BmGtsf1L binding. Our study reveals that a single eTudor domain within BmVreteno provides two binding interfaces and thereby interconnects piRNA‐loaded BmAgo3 and BmGtsf1L.
Mechanistic basis of ligand efficacy in the calcium‐activated chloride channel TMEM16A
Authors: Lam Andy KM , Dutzler Raimund 
Published: November 2023

AbstractAgonist binding in ligand‐gated ion channels is coupled to structural rearrangements around the binding site, followed by the opening of the channel pore. In this process, agonist efficacy describes the equilibrium between open and closed conformations in a fully ligand‐bound state. Calcium‐activated chloride channels in the TMEM16 family are important sensors of intracellular calcium signals and are targets for pharmacological modulators, yet a mechanistic understanding of agonist efficacy has remained elusive. Using a combination of cryo‐electron microscopy, electrophysiology, and autocorrelation analysis, we now show that agonist efficacy in the ligand‐gated channel TMEM16A is dictated by the conformation of the pore‐lining helix α6 around the Ca2+‐binding site. The closure of the binding site, which involves the formation of a π‐helix below a hinge region in α6, appears to be coupled to the opening of the inner pore gate, thereby governing the channel's open probability and conductance. Our results provide a mechanism for agonist binding and efficacy and a structural basis for the design of potentiators and partial agonists in the TMEM16 family.
НОВА ПРОЄКТНА МЕТОДИКА РОЗРАХУНКУ ОСНОВНИХ ПАРАМЕТРІВ СОНЯЧНОГО КОЛЕКТОРА
Authors: Габрінець В.О. , Накашидзе Л.В. 
Published: November 2023

Анотація. Запропонована нова методика розрахунку конструкції сонячного геліоколлектора, який перетворює сонячну енергію для отримання тепла. В ній пропонується застосовувати коефіцієнт заміщення, який означає відношення теплоти, отриманої системою в результаті сонячного випромінювання, до повного теплового навантаження всієї системи, тобто приймити до уваги не тільки теплові втрати самого геліоколектора, но і втрати самої системи на яку працює геліоколлектори. Це дозволяє розраховувати ефективність усієї системи перетворення сонячної енергії в теплову. Пропонується класифікація систем, які використовують сонячну енергію з використанням колектора для вироблення тепла, Розглянуто різні варіанти принципових схем геліоколектора, а також різні варіанти його теплосприймаючої поверхні. Розглянуто вплив конструкції геліоколлектора на величину теплових втрат. Під час визначення коефіцієнта теплових втрат геліококолектора застосовується метод послідовних наближень. Під час визначення теплових втрат необхідно враховувати нерівномірність розподілу температури в перерізі геліоколектора. Для цього пропонується вираз для відповідного коефіцієнта. Зниження теплових втрат поглинаючої поверхні досягають встановленням прозорого покриття, яке повинно добре пропускати крізь себе сонячне випромінювання і утворювати повітряний прошарок, виконуючий роль термічного опору. Крім цього, покриття захищає теплосприймаючий елемент від дощу, снігу, граду і тому має бути достатньо міцним. Запропонована проектна методика розрахунку сонячного колектора дозволяє отримати такі його параметри: площу геліоколектора, кількість модулей, що входять до складу геліоколектора , теплові втрати в геліоколекторі, корисну потужність, надану теплоносію в геліоколекторі, температуру теплоносія на виході зі сонячного колектора. Вміння якісно розраховувати такий основний елемент сонячної енергоустановки, як геліоколектор, має велике практичне значення. Воно дозволяє швидко і досить точно розраховувати основні параметри всій сонячної енергетичної установки (СЕУ) в різних випадках її широкого застосування.
The Relationship of Sweet Potato Germplasm Based on Morphological Characters
Authors: Rahajeng Wiwit , Restuono Joko , Indriani Febria C. 
Published: November 2023

Information about the morphological and genetic characteristics of sweet potato (Ipomoea batatas) is important in the variety development program. This experiment aimed to determine the diversity and genetic relationship of sweet potato accessions from NTT based on morphological character data. The research was carried out at Kendalpayak Research Station, Malang, East Java from April - September 2019. The materials used were seventy-one sweet potato germplasm from the ILETRI collection (collected from NTT). Each accession was planted on a 1 m × 5 m plot size, with a spacing of 100 × 20 cm (single row). Fertilization was carried out using a dose of “100 kg urea + 100 kg SP36 + 200 kg KCl ha-1”. The observed characters were the vines length, growth type, internode length, leaf length, leaf width, leaf size, leaf bone color, leaf shape, leaf characteristics, lobes number, lobe shape, mature leaf color, shoot color, pigmentation of petiole, young stems pigmentation (dominant and secondary color), young leaves feathers, tuber skin color, tuber flesh color, weight of canopy, number of tubers perplot, weight of tubers perplot, number and weight of tubers perplot. Cluster analysis was carried out using the Minitab 17 program. There was morphological diversity in seventy-one accessions of sweet potato germplasm from NTT. Principal component analysis resulted in seven main components with the proportion of diversity 76.3%. cluster analysis, seventy-one accessions of sweet potato germplasm were divided into fifteen accession groups on the basis of 80% degree. Characteristics of shoot color, mature leaf color, leaf size, petiole pigmentation, and leaf bone color contributed greatly to the total diversity.
Effect of Mercury Stress on the Growth and Lipid Content of Euglena sp. and Echinodorus palaefolius
Authors: Wardana Wisnu Eka , Tantri Dea Hastaning , Afifah Marshanda Nur Roosyana , Aini Hasna Nur , Siswanti Dwi Umi , Maghfiroh Khusnul Qonita , Erfianti Tia , Amelia Ria , Kurnianto Dedy , Suyono Eko Agus 
Published: November 2023

One way to reduce the adverse effects of the heavy metals mercury in the aquatic environment are using organisms to break down or convert toxic substances into non-toxic forms, either by phytore- mediation or phycoremediation. This research aimed to analyze the growth and lipid content of Euglena sp. after mercury exposure. This research also aimed to analyze the growth of E. palaefolius which is associated with Euglena sp. In this study, the bioremediation ability of Euglena sp. and Echinodorus palaefolius through treatment with mercury concentrations of 5 ppm, 10 ppm, 15 ppm, and 20 ppm, as well as association and non-association treatments. The parameters are the growth of Euglena sp. and the association between Euglena sp. andE. palaefolius measurement and lipid content. The result of the growth of Euglena sp. experienced a significant increase. Lipid content in Euglena sp. was also seen high at 10 ppm mercury concentration. In E. palaefolius, the ability to adsorb heavy metals was also shown by the large diameter of the stems and also the plant growth which has optimal growth in the treatment of 10 ppm mercury stress.
Analisis Daya Keluaran pada Kinerja Motor Listrik Tipe Synchronous Berbasis Komputasional
Authors: Ferdyanto Ferdyanto , Farha Auditya , Julian James , Wahyuni Fitri , Rizal Reda 
Published: November 2023

Masalah yang menjadi pertimbangan dalam penggunaan motor listrik adalah daya keluaran yang dihasilkan pada saat motor bekerja. Penelitian ini berfokus pada analisis daya keluaran Permanent Magnet Synchronous Motor (PMSM) yang akan diuji pada kecepatan tertentu serta ditinjau terhadap perubahan sudut torsi tiap putaran. Tujuan penelitian ini adalah mendapatkan analisis daya keluaran dengan karakteristik yang stabil serta kinerja PMSM yang menghasilkan efisiensi sesuai standar. Penelitian menggunakan metode komputasi dengan model finite element analysis (FEA) yang dilakukan dengan menggunakan software. Hasil dari penelitian ini diperoleh performa PMSM dengan arus input sebesar 1.5 kA pada setiap fasa sehingga motor dapat beroperasi dengan kecepatan 13300 rpm serta menghasilkan torsi sebesar 164 Nm. Fluks magnet yang terbentuk berada dalam kondisi stabil dengan nilai 0.02 Wb dan membentuk distribusi medan magnet yang merata pada setiap kumparan stator. Rata-rata daya keluaran maksimum yang dihasilkan jangkar rotor adalah 107.6 kW pada sudut torsi 114°. Penelitian telah berhasil memperoleh analisis daya keluaran pada PMSM melalui metode komputasi. Hasil analisis tersebut dapat menjadi acuan dalam proses manufaktur PMSM. The problem that is considered in the use of electric motors is the output power generated when the motor is working. This research focuses on analyzing the output power of a Permanent Magnet Synchronous Motor (PMSM) which will be tested at a certain speed and looking at changes in torque angle for each rotation. The aim of this research is to obtain an output power analysis with stable characteristics and PMSM performance that produces efficiency according to standards. The research uses computational methods with finite element analysis (FEA) models which are carried out using software. The results of this research obtained PMSM performance with an input current of 1.5 kA in each phase so that the motor can operate at a speed of 13300 rpm and produce a torque of 164 Nm. The magnetic flux formed is in a stable condition with a value of 0.02 Wb and forms an even magnetic field distribution in each stator coil. The average maximum output power produced by the rotor armature is 107.6 kW at a torque angle of 114°. Research has succeeded in obtaining output power analysis on PMSM through computational methods. The results of this analysis can be used as a reference in the PMSM manufacturing process.
Geotechnical properties of natural sand-mixed granulated blast furnace slag applied to the sand compaction pile method
Authors: Nguyen Thi Thanh Nhan , Matsuda Hiroshi , Duong Trung Quoc , Tran Xuan Thach , Tran Thi Phuong An , Tran Thi Ngoc Quynh , Tran Thanh Nhan , Pham Van Tien , Nguyen Thi Le Huyen 
Published: November 2023

Granulated blast furnace slag (GBFS) has more advantageous properties over natural sand, such as lightweight, higher shear strength, and higher permeability. Therefore, it is regarded as a potentially promising substitute for natural sand in the ground improvement using the sand compaction pile (SCP) method. Due to the relatively rapid solidification, however, which is induced by the latent hydraulic property of GBFS, the permeability of the installed GBFS compaction pile (GBFSCP) decreases quickly, and such an application becomes difficult for the case of SCP with low sand replacement area ratio (LSRAR). So, to decelerate the permeability reduction of GBFSCP, different GBFSs, and natural sands were collected in Japan and Vietnam, and the specimens were prepared by changing the mixing ratio of sand to GBFS from 20% to 80%. Then, firstly, fundamental geotechnical properties of sand-mixed GBFS without hydration were observed. Secondly, the specimens of sand-GBFS mixture were cured in seawater at the temperature of 80°C for 3 days to 380 days. The unconfined compressive strength, permeability, and hydration reaction rate of the hydrated sand-mixed GBFS specimen were measured.
In conclusion, the geotechnical properties of the unhydrated sand-mixed GBFS become more advantageous than those of natural sand. In addition, by mixing with natural sand, the hydration reaction rate and the solidification of GBFS are controlled. This means that it is possible to keep the compressive strength and the permeability at the predetermined conditions and, in turn, to satisfy requirements for the SCP method with LSRAR. In addition, a simple estimation method of the unconfined compressive strength was proposed for the hydrated sand-mixed GBFS specimen.
Предварительные данные о флоре цианобактерий российских вод Финского залива Балтийского моря
Authors:   Российский государственный педагогический университет им. А.И. Герцена, Горин К.К. ,   Ботанический институт РАН им. В.Л. Комарова
Published: November 2023

Цианобактерии – важная для водных экосистем группа фотоавтотрофных организмов, роль которой в вегетационный период зачастую является определяющей для биотопов Финского залива. Изучение их флоры не утратило своей актуальности. На протяжении всего периода исследований микроводорослей этого района, планктонные цианобактерии чаще всего являлись объектами комплексных гидробиологических исследований, осуществляющихся при строительных, гидротехнических, природоохранных и других работах, а также мониторинге в акватории залива [4,5,6,7]. Исследования бентосных сообществ микроводорослей имели скорее эпизодический характер, и к настоящему времени, сведения о их видовом составе в восточной части Финского залива ограничивались небольшим числом публикаций [1,2,3]. Материалом для настоящей работы послужили пробы, собранные летом в период с 2017 по 2021 гг. на станциях в прибрежных зонах северного и южного побережий Финского залива, архипелага Берёзовые острова, а также островов Гогланд и Мощный. В результате инвентаризации таксономического состава, по оригинальным и литературным данным, составлен список из 243 видов и внутривидовых таксона цианобакторий из 77 родов, 31 семейства, 13 порядков и одного класса. Наибольшим количеством видов представлены порядки Chroococcales и Nostocales - 74 и 67 видов, соответственно. Меньшее количество видов включали порядки Oscillatoriales (37 видов), Synechococcales (17 видов), Leptolyngbyales (14 видов) и Pseudanabaenales (12 видов). Остальные порядки содержали от 1 до 6 видов. По отношению к местообитанию выявлено 104 планктонных вида, 80 бентосных видов, произрастающих на различных субстратах. Планктонно-бентосные цианопрокариоты были представлены 58 видами, в основном это представители родов Chroococcus Nägeli, Merismopedia Meyen, Lyngbya C.Agardh ex Gomont, Oscillatoria Oscillatoria Vaucher ex Gomont, Phormidium Kützing ex Gomont, и др. 4 вида являлись эндоглойными, живущими в слизи других цианобактерий и водорослей. Для 173 видов были установлены галобные характеристики. Большинство из них – 81 вид являлись пресноводно-солоноватоводными. На втором месте по количеству видов были пресноводные цианобактерии - 67 видов. Доля солановатоводно-морских видов во флоре была не высока и составляла 5% - 13 видов. Всего 3% видов от общего числа относятся к эвригалинным и способны произрастать в широком диапазоне солёности.	По составу зонально-географических элементов были выявлены виды являющиеся аркто-бореальными, бореальными, бореально-тропическим, голарктическими, голарктическо-тропическими, голарктическо-палеотропическими, голарктическо-неотропическими, голарктическо-австралийскими, и космополитными.
Source Spectra of Pg and Lg Waves from North Korean Nuclear Tests Estimated Using a Nonmodel-Based Approach
Authors: Jin Ping , Xu Henglei , Ding Shiban , Wang Weiping , Zhu Haofeng 
Published: November 2023

ABSTRACT
In this article, we demonstrate that source spectra for regional seismic waves generated by an underground explosion may be uniquely extracted using a simple linear regression method between the geometrical spreading corrected displacement spectra and epicentral distances as long as the recording stations are well distributed in distances. Using this method, source spectra of Pg and Lg waves from North Korean nuclear tests conducted between 2009 and 2017 are estimated, respectively, and are compared to test the conjecture of Fisk (2006) as well as to determine the relative size between the seismic moment of Lg waves and that of compressional waves. For a specified explosion, the resultant Pg and Lg source spectra approximately have equal low-frequency source level. Based on this result, it is estimated that for the explosions seismic moment of Lg waves would be about 35% of that of compressional waves. In addition, it is shown the Fisk conjecture approximately stands, as for a specified explosion the observed Pg and Lg source spectra share the same manner of high-frequency rolloff as f−2, the source corner frequency of Lg waves is smaller than that of Pg waves by a factor of about 1/3, and the Pg source spectrum exhibits a prominent overshoot, whereas it is not obvious in corresponding Lg source spectrum. The new method for source spectra estimation may have a promising application prospect in monitoring studies. As an example, it is shown that the yield and even buried depths of the North Korean nuclear explosions may be reasonably estimated by fitting the observed Pg source spectra using an MM71- (Mueller and Murphy, 1971) and DJ91 (Denny and Johnson, 1991)-based hybrid source model suggested by Ford and Walter (2013).
Physician Burnout as Reflected by Patients – the Effect of COVID-19 on Patients’ Perspective
Authors: Tal Orna , Ravid Arnon , Bargil Shlomit , Epel Zaklin , Avdayev Mira , Bitan Michal 
Published: November 2023

Physicians exposed to a risky environment experience burnout, which negatively impacts patient care, stress decreases compassion, harms patient-doctor communication and may jeopardize patient safety. Patients are directly exposed to their physicians&#039; overload and can sense their exhaustion, making them a sensitive seismograph of burnout. This study aimed to compare patients&#039; standpoints towards physicians&#039; burnout before and during the COVID-19 pandemic, characterizing patients&#039; perceptions according to the 4 components of burnout.
Two cross-sectional surveys (February-March 2018 and 2021) were conducted. 314 responders, 133 in 2018 and 181 in 2021. Physician burnout, accountability, empathy towards doctors (&quot;mirror reflection&quot;) and the expected patient-doctor partnership were measured.
Patients&#039; perception of physicians&#039; burnout was significantly associated with urban community, education and religiosity. Perceived accountability was associated with female gender and education. Empathy was associated with employment and gender.
Patients described physicians&#039; burnout as significantly higher prior to the COVID-19 pandemic. Physicians&#039; accountability, empathy for doctors, and perceived partnership were more intense in 2021, implying that the emergency emphasized the physician&#039;s role and the patient-doctor relationship. Global disasters may have an unexpected positive effect on how patients understand the role and duty of physicians and the health system.
Association of WBC Levels With All-Cause Mortality in ICU Patients Receiving CRRT: A Retrospective Cohort Study
Authors: Huang Haowen , Huang Feng , Zhou Jie , Zhang Zhiqin , Ren Yuan , Chang Sansi , Xue Jun 
Published: November 2023

Background
Acute kidney injury (AKI) is a common comorbidity in the intensive care unit (ICU), with higher mortality rate among those who receive continuous renal replacement therapy (CRRT). This study aimed to investigate the association of white blood cell (WBC) levels with all-cause mortality in AKI patients undergoing CRRT.
Method
We conducted a post hoc analysis of ICU patient data from multiple centers in Korea. First, the levels of WBCs were classified into four groups based on quartiles. Second, the relationship between different WBC levels and mortality was investigated with the Kaplan-Meier (K-M) survival curves, Cox proportional hazards model, and generalized additivity model (GAM). Finally, we used multiple imputation to avoid bias caused by non-randomized missing data. The endpoints were short-term (28 days) and long-term (90 days) mortality.
Result
In our analysis, a total of 1,100 ICU patients were included. Among them, 670 (60.90%) and 781 (71.00%) died within 28 and 90 days, respectively. K-M survival curves showed that patients with the lowest WBC levels had the highest risk of mortality for both outcomes. Adjusted multivariate Cox regression analysis confirmed that compared with the reference level (quartile 2), low WBC levels increased the risk of both 28-day mortality (quartile 1, HR = 1.43, 95% CI: 1.13–1.83, P = 0.004) and 90-day mortality (quartile 1: HR = 1.32; 95% CI: 1.05-1.66, P = 0.016). GAM analysis showed a non-linear, U-shaped relationship between pre-CRRT WBC levels and all-cause mortality (non-linear P &lt; 0.05).
Conclusion
The baseline WBC level is an independent predictor of prognosis for ICU patients receiving CRRT. Patients in the lowest WBC quartile had the highest risk of all-cause mortality. These results provide further evidence that early observation and intervention may have a prognostic value for these high-risk patients.
Increasing the Oil Production Coefficient of Oil and Gas Reservoirs Operating in The Dissolved Gas Mode
Authors: Mammadova G. G. , Abbasova S. V. , Abbaszade E. E. 
Published: November 2023

Abstract
Depending on the daily gas production, the character of the pressure dynamics in the field and the movement of the gas-water contact should be determined in order to provide a project for the development of gas fields. The change of those factors mainly depends on the regime of the bed.
In the dissolved gas regime, the only driving force is the elastic energy of gas dissolved in oil.
When gas-cut liquid (oil) moves in the formation, the relative permeability of the formation for gas and oil depends on its oil saturation factor. The saturation coefficient depends on the amount of oil and gas extracted from the reservoir and reservoir pressure. Therefore, since the movement under the dissolved gas drive mode is complex and undetermined, approximate methods to solve hydrodynamic problemsare used.