bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appressorium formation is regulated by the Msb2‐ and Sho1‐dependent hierarchical transcriptional network in Colletotrichum gloeosporioides
Authors: Liu Na , Meng Fanli , Tian Chengming 
Published: November 2023

AbstractColletotrichum gloeosporioides, which penetrates the plant hosts via special infection structures called appressoria, causes anthracnose in many plants worldwide. However, the biological mechanism underlying appressorium formation is not fully characterized in this pathogen. In the present study, the overall gene expression pattern of appressorium formation was studied by targeted gene deletion and expression analysis during the conidial germination of wildtype and ΔCgMsb2Sho1 double mutant strains of C. gloeosporioides on hydrophobic surfaces. Significant transcriptional changes were detected for nearly 41.9% and 45.4% of the genes in the wildtype and ΔCgMsb2Sho1 strains, respectively. Two genes encoding the cAMP‐dependent protein kinase regulatory subunit and adenylate cyclase had higher expression in the wild type than in the ΔCgMsb2Sho1 mutant; cAMP then activates the mitogen‐activated protein kinase (MAPK) signalling pathway. However, we revealed that genes involved in the MAPK signalling pathway may inhibit appressorium formation by encoding proteins that disrupt the downstream signal transduction process after recognition of the host by the cell membrane surface receptor proteins mucin (Msb2) and the sensor protein Sho, encoded by CgMsb2 and CgSho1, respectively. Finally, the regulation of transcription factors on lipid metabolism and plant cell wall‐degrading enzymes was affected. The results provide a comprehensive overview of the changes in the expression of important genes during the early stage of appressorium formation, which may be useful for understanding the molecular mechanism of appressorium formation in C. gloeosporioides.
Legal Interpretation, Conceptual Ethics, and Alternative Legal Concepts
Authors: Plunkett David 
Published: November 2023

AbstractWhen legal theorists ask questions about legal interpretation—such as what it fundamentally is, what it aims at, or how it should work—they often do so in ways closely tethered to existing legal practice. For example: they try to understand how an activity legal actors (purportedly) already engage in should be done better, such as how judges can better learn about the content of the law. In this paper, I discuss a certain kind of “conceptual ethics” approach to thinking about legal interpretation, which is less tethered to existing legal practice (or the existing meaning of core pieces of legal terminology). The approach I explore asks questions about legal interpretation in a way that is tethered to what legal (or “legal‐ish”) concepts people should deploy, as part of arguments on behalf of engaging in legal (or “legal‐ish”) practices different from our current ones. In exploring this approach, I aim to help us better understand the landscape of philosophical issues about legal interpretation, including parts of it that I think have been underexplored.
‘Walking‐the‐talk’: How to co‐design an innovative and sustainable integrated care learning programme
Authors: Siddiqui Nazlee 
Published: November 2023

AbstractIntegrated care facilitates better outcomes for patients and their family, health workforce and health and social care systems. Accordingly, integrated care is a global and inherent area of work for health professionals. Despite this, formal learning programmes for workforce development in integrated care are still rare. In this paper, I have shared lessons about developing an innovative and sustainable integrated care learning programme for teachers, practitioners and learners in integrated care discipline. These lessons are drawn from my experience of leading an integrated care specialisation programme (that sits at Australian Qualification Framework Level 9) at a public university in Australia. An integrated care learning programme should be designed with appropriate philosophies (e.g., social constructivism and connected learning) and relevant multidisciplinary content that addresses deficiencies in practices of integrated care. Moreover, the programme should aim for sustainability, through financial feasibility and continuity with offering of creative and effective solutions in integrated care. Similarly, the programme should have a suit of curriculum development and teaching initiatives to promote genuine and collaborative learning. Throughout this learning journey, it would be critical for teachers to engage in ‘walking‐the‐talk’, that is, genuinely collaborate with patients, practitioners and students, for successful delivery of an innovative and sustainable learning programme.
IST1 regulates select recycling pathways
Authors: Clippinger Amy K. , Naismith Teresa V. , Yoo Wonjin , Jansen Silvia , Kast David J. , Hanson Phyllis I. 
Published: November 2023

AbstractESCRTs (Endosomal Sorting Complex Required for Transports) are a modular set of protein complexes with membrane remodeling activities that include the formation and release of intraluminal vesicles (ILVs) to generate multivesicular endosomes. While most of the 12 ESCRT‐III proteins are known to play roles in ILV formation, IST1 has been associated with a wider range of endosomal remodeling events. Here, we extend previous studies of IST1 function in endosomal trafficking and confirm that IST1, along with its binding partner CHMP1B, contributes to scission of early endosomal carriers. Functionally, depleting IST1 impaired delivery of transferrin receptor from early/sorting endosomes to the endocytic recycling compartment and instead increased its rapid recycling to the plasma membrane via peripheral endosomes enriched in the clathrin adaptor AP‐1. IST1 is also important for export of mannose 6‐phosphate receptor from early/sorting endosomes. Examination of IST1 binding partners on endosomes revealed that IST1 interacts with the MIT domain‐containing sorting nexin SNX15, a protein previously reported to regulate endosomal recycling. Our kinetic and spatial analyses establish that SNX15 and IST1 occupy a clathrin‐containing subdomain on the endosomal perimeter distinct from those previously implicated in cargo retrieval or degradation. Using live‐cell microscopy, we see that SNX15 and CHMP1B alternately recruit IST1 to this subdomain or the base of endosomal tubules. These findings indicate that IST1 contributes to a subset of recycling pathways from the early/sorting endosome.
Models of care and the advanced practice nurse role in caring for children and adolescents with a cancer predisposition syndrome: a scoping review protocol
Authors: Grant Andrew M. , Signorelli Christina , Taylor Natalie , de Graves Sharon , Tucker Kathrine M. , Cruickshank Marilyn 
Published: November 2023

Objective:
This scoping review will examine the literature describing models of care, barriers and facilitators of care, and gaps in care delivery for children and adolescents with a cancer predisposition syndrome (CPS). It will also explore how advanced practice nurses contribute to the delivery of care for children and adolescents with a CPS.

Introduction:
Cancer remains a leading cause of death in children and adolescents. Pediatric CPS clinics proactively aim for early diagnosis or prevention of cancer in children and adolescents with a CPS. Additionally, the holistic well-being of individuals requires a multidisciplinary team, including advanced practice nurses, to manage their complex health care needs.

Inclusion criteria:
This review will consider both published and unpublished literature exploring aspects of models of care and the role of the nurse in pediatric CPS clinics. Literature published in English from 1991 onward will be considered.

Methods:
This scoping review will follow the JBI methodology for scoping reviews. The review will include searches in MEDLINE, Embase, and CINAHL Complete. Gray literature searches will be conducted in OAIster and Social Science Research Network, as well as websites of hospitals in the USA and the UK with large pediatric cancer centers. Two reviewers will screen titles, abstracts, and full-text articles. An extraction table will be used to extract relevant data from all included articles and facilitate data analysis. Results will be presented in narrative and tabular format.

Review registration:
Open Science Framework osf.io/axkp7/
Verification and Validation of Rotating Machinery Using Digital Twin
Authors: Yanik Yasar , Ekwaro-Osire Stephen , Dias João Paulo , Porto Edgard Haenisch , Alves Diogo Stuani , Machado Tiago Henrique , Bregion Daniel Gregory , de Castro Helio Fiori , Cavalca Katia Lucchesi 
Published: November 2023

Abstract
Rotating machinery has extensive usage in industrial applications, either as leading equipment (power plants) or as auxiliary equipment (oil and gas exploitation). These highly complex systems demand expensive maintenance programs due to the high costs of eventual shutdown. Consequently, critical fault diagnosis and prognosis are essential during the operation of those systems. Fault identification and classification demand robust verification of codes and calculations and a discerning validation of numerical models used for rotating machinery. Hence, verification and validation (V&V) are an essential initial service for a digital twin (DT), offering some advantages in this application. In this context, the following research question is proposed: Does V&V using DT improve data access and reduce the effort of data exchange? The following objectives are created to address the research question: perform a code verification, conduct the calculation verification, validate the models using two different validation approaches, and demonstrate easy access to asset data. For this study, two identical hydrodynamic bearings and a noncentral disk were considered, representing a laboratory experimental setup. The validation metric requirement is promisingly satisfied for the disk and bearings according to validation Approaches 1 and 2. Furthermore, validation Approach 2 generates even more successful results than approach 1. Accurate estimation and reliable interpretation of the numerical model outcomes encourage the application of DT for future fault diagnosis and prognosis.
A combination of burn wound injury and
Pseudomonas
infection elicits unique gene expression that enhances bacterial pathogenicity
Authors: Kambouris Adrienne R. , Brammer Jerod A. , Roussey Holly , Chen Chixiang , Cross Alan S. 
Published: November 2023

ABSTRACT

Burns are a leading cause of morbidity and mortality worldwide with the most common cause of death resulting from sepsis, often from
Pseudomonas aeruginosa
. We previously reported that a non-lethal flame burn induced an altered host immune response. Using this model, gene expression in both the murine host and
P. aeruginosa
was measured using a NanoString custom probe panel. We observed differing patterns of gene expression in both host and
P. aeruginosa
in the skin, blood, liver, and spleen of mice that were burned and/or infected, compared to mice that were neither burned nor infected (i.e., Sham). In mice that were both burned and infected (B/I), we observed changes in gene expression in both the host and
P. aeruginosa
that were distinct from all other treatment conditions. These data suggest that the combination of the burned state and superimposed infection affects both host and pathogen gene expression to increase infection propensity. Gene transcription significantly changed from 6 to 24 h post-B/I in each tissue. Finally, inhibiting IL-10 signaling or co-administering arginine at the time of
P. aeruginosa
infection prolonged or restored survival in an otherwise 100% fatal burn and infection model. These findings suggest that disease states such as burns may differentially alter innate immune response gene expression in both a host- and pathogen-specific manner.

IMPORTANCE

The interaction between an underlying disease process and a specific pathogen may lead to the unique expression of genes that affect bacterial pathogenesis. These genes may not be observed during infection in the absence of, or with a different underlying process or infection during the underlying process with a different pathogen. To test this hypothesis, we used Nanostring technology to compare gene transcription in a murine-burned wound infected with
P. aeruginosa
. The Nanostring probeset allowed the simultaneous direct comparison of immune response gene expression in both multiple host tissues and
P. aeruginosa
in conditions of burn alone, infection alone, and burn with infection. While RNA-Seq is used to discover novel transcripts, NanoString could be a technique to monitor specific changes in transcriptomes between samples and bypass the additional adjustments for multispecies sample processing or the need for the additional steps of alignment and assembly required for RNASeq. Using Nanostring, we identified arginine and IL-10 as important contributors to the lethal outcome of burned mice infected with
P. aeruginosa
. While other examples of altered gene transcription are in the literature, our study suggests that a more systematic comparison of gene expression in various underlying diseases during infection with specific bacterial pathogens may lead to the identification of unique host-pathogen interactions and result in more precise therapeutic interventions.
Whole-genome sequence of
Periconia
sp. strain TS-2, an ascomycete fungus isolated from a freshwater outflow and capable of Mn(II) oxidation
Authors: Tsushima Shihori , Kanaly Robert A. , Mori Jiro F. 
Published: November 2023

ABSTRACT

Members of the genus
Periconia
are commonly found as plant-associated filamentous fungi. Here, the first draft genome sequence of a new
Periconia
strain, TS-2, that was isolated from freshwater outflow sediment and possesses the ability to oxidize dissolved Mn(II), was obtained and has an estimated size of 40.7 Mb.
RNA virus diversity in three parasitoid wasps of tephritid flies: insights from novel and known species
Authors: Zhang Wei , Li Rong , Li Shuai , Li Shao-Yang , Niu Jinzhi , Wang Jin-Jun 
Published: November 2023

ABSTRACT

Parasitoid wasps are a diverse group of parasitoid insects that play a crucial role in biological control and integrated pest management programs due to their wide range of host species and complex behaviors. Previous studies have reported that select RNA viruses in parasitoid wasps can affect either the host-parasitoid wasp or the pest host parasitized by the wasps. Therefore, a study of the dynamics of RNA viruses in parasitoid wasps is essential for artificial breeding programs and improving their parasitic capabilities. In this study, we detected the presence of nine novel and three known RNA viruses with complete genomes in three parasitoid wasp species:
Diachasmimorpha longicaudata
,
Fopius arisanus
, and
Spalangia endius
. These wasps are parasitoids that can parasitel tephritid flies, one of the most important pest groups of fruits and vegetables. In
D. longicaudata
, the small RNAs derived from the viruses exhibited distinct peaks at 22 nt and were symmetrically distributed across the viral genome. These findings indicate that these viruses can activate the host’s RNAi immune response. PCR detection further revealed that DlNaLV efficiently infects female individuals of
D. longicaudata
with 100% efficiency, and ZcNLV is capable of infecting both
D. longicaudata
and its host,
Zeugodacus cucurbitae
. Our findings contribute to a better understanding of RNA viruses in parasitoid wasps and lay the foundation for future research on the triple-level trophic relationships between tephritid flies, parasitoid wasps, and RNA viruses.

IMPORTANCE

Parasitoid wasp populations have developed persistent beneficial symbiotic relationships with several viruses through repeated evolution. However, there have been limited reports on RNA viruses in parasitoid wasps of tephritid flies, a significant pest group affecting fruits and vegetables. This study explores the diversity of RNA viruses in three parasitoid wasps of tephritid flies and highlights the potential biological significance of specific viruses in
Diachasmimorpha longicaudata
. These findings have important implications for the development of sustainable pest management strategies and the enhancement of artificial rearing techniques for parasitoid wasps.
Impact of the COVID-19 pandemic on the cost of chronic diseases treatment and care at public hospitals in Wallaga zones, Oromia Regional State, Ethiopia: a hospital-based, cross-sectional study
Authors: Terefa Dufera Rikitu , Tesfaye Edosa , Tolessa Belachew Etana , Desisa Adisu Ewunetu , Olani Wolkite , Fetensa Getahun , Chego Melese , Abdisa Eba , Turi Ebisa , Bekuma Tariku Tesfaye , Getachew Motuma , Tesfaye Lensa , Tilahun Temesgen 
Published: November 2023

ObjectiveGlobally, around one-third of the population has at least one long-term health condition that could be affected by the COVID-19 pandemic. Despite the fact that studies have revealed the direct impact of COVID-19 on healthcare provision and utilisation, the impact of the pandemic on the cost of chronic disease treatment and care from a patient perspective was scanty. So, the study aimed to determine the impact of the COVID-19 pandemic on cost of chronic diseases treatment and care at public hospitals in Wallaga zones, Oromia Regional State, Ethiopia, from 1 August to 31 August 2020.MethodsAn institutional-based cross-sectional study design was used, and the sample size for the study (n=642) was determined using a single population mean formula. Data were collected using interviews and analysed using SPSS V.25. Descriptive statistics were performed, and the cost of follow-up care before and after the pandemic was compared using a related-samples Wilcoxon signed-rank test, declaring the level of significance of the median cost difference at p<0.05.ResultsA total of 642 patients were included in the study, of whom 605 (94.2%) responded to the interviews. There was a significant median cost difference (n=593, Z=5.05, p=0.001) between the cost of chronic diseases among follow-up patients during the pandemic and the costs incurred by these patients before the pandemic.ConclusionThe cost of follow-up care among chronic disease patients during the COVID-19 pandemic was significantly higher compared with before the pandemic era. Therefore, healthcare providers should arrange special fee waiver mechanisms for chronic disease healthcare costs during such types of pandemics and provide the services at proximal health facilities.
Precancerous cervical lesion screening acceptance among women in Eastern Ethiopia
Authors: Wakwoya Elias Bekele , Sadi Chala Gari , Sendo Endalew G 
Published: November 2023

ObjectiveThis study assessed perceived barriers, precancerous cervical lesion screening acceptance, and associated factors among women in Eastern Ethiopia.SettingThis study was conducted in Hiwot Fana Specialized Hospital and Jugal Hospital.Study designThis study is a facility-based cross-sectional study.Study participantsThis study included 1181 women aged 25–49 years. Bivariate and multivariable logistic regression was used to evaluate the relationship between variables and control confounders.ResultsNearly half of the participants (587 or 49.7%) agreed to be screened for precancerous cervical lesions. Seventy-six per cent of those checked were negative for visual inspection with acetic acid, 18.5% were positive, and 5.7% had cancer-like lesions. In multivariable analysis, fear of discomfort from the screening procedure, having a male screener, and embarrassment were the perceived barriers that were inversely associated with screening acceptance.ConclusionsThe uptake of the screening service in the study area was not satisfactory, indicating that the programme was underutilised in the area.
Design and evaluation of a digital health intervention with proactive follow-up by nurses to improve healthcare and outcomes for patients with breast cancer in Mexico: protocol for a randomised clinical trial
Authors: Contreras Sánchez Saúl Eduardo , Doubova Svetlana V , Grajales Álvarez Rocío , Dip Borunda Abdel Krim , Martínez Pineda Wendy Jazmín , Nuñez Cerrillo Jose Gustavo , Silva Bravo Fernando , Zalapa Velázquez Rita , Gutiérrez De la Barrera Marcos , Leslie Hannah H 
Published: November 2023

IntroductionNearly 30 000 Mexican women develop breast cancer annually, frequently presenting unmet supportive care needs. In high-income countries, incorporating electronic patient-reported outcomes (ePROs) into cancer care has demonstrated potential for increasing patient-centred care and reducing unmet needs. No such ePRO interventions have been implemented in Mexico. This paper presents the study protocol for designing and evaluating an ePRO digital health application combined with proactive follow-up by nurses.Methods and analysisWe designed a two-component intervention for women receiving breast cancer treatment: a responsive web application for monitoring ePROs and clinical algorithms guiding proactive follow-up by nurses. We will conduct a pilot test of the intervention with 50 patients with breast cancer for 6 weeks to assess feasibility and adjust the application. We will conduct a parallel arm randomised controlled trial assigning 205 patients each to intervention and control in one of Mexico’s largest public oncology hospitals. The intervention will be provided for 6 months, with additional 3 months of post-intervention observation. The control group will receive usual healthcare and a list of breast cancer information sources. Women diagnosed with stages I, II or III breast cancer who initiate chemotherapy and/or radiotherapy will be invited to participate. The primary study outcome will be supportive care needs; secondary outcomes include global quality of life and breast symptoms. Information on the outcomes will be obtained through web-based self-administered questionnaires collected at baseline, 1, 3, 6 and 9 months.Ethics and disseminationThe National Research and Ethics Committees of the Mexican Institute of Social Security approved the study (R-2021-785-059). Participants will sign an informed consent form prior to their inclusion. Findings will be disseminated through a policy brief to the local authorities, a webinar for patients, publications in peer-reviewed journals and presentations at national and international conferences.Trial registration numberNCT05925257.