bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and characterisation of potential bacterialpathogens from Oreochromis niloticus (Linnaeus, 1758) of Lake Hawassa, Ethiopia
Authors: Eregena Alazar , Natarajan P. , Bedewi Zufan 
Published: February 2024

The aim of the current study was to isolate and identify potential bacterial pathogens from Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) in Lake Hawassa, Ethiopia. The study was conducted during January to December 2021. A total of 360 Nile tilapia were sampledfrom Lake Hawassa and subjected to bacteriological investigations. The results showed 64.2% prevalence of bacterial infection in Nile tilapia from the lake. Potential bacterial pathogens such as Aeromonas hydrophila, Escherichia coli, Edwardsiella tarda, Vibrio sp,,Bacillus sp., Staphylococcus sp., Salmonella sp., Flavobacterum sp., Pseudomonas sp. and Shigella sp. were isolated. The prevalence of infection in female fish (52.4%) was higher compared to male fish (47.6%). Comparatively higher prevalence was recorded in thesmaller fish group than in the larger ones. The average bacterial loads recorded in various organs such as intestine, skin, gill, liver and kidney were, 4.86±0.56 x 105, 4.72±0.48 x 105, 4.14±0.53 x 105, 3.45±0.23 x 105 and 2.63±0.324 x 105 CFU ml-1 respectively. Significantly high bacterial loads were recorded from the intestine (5.81±0.01 x 105 CFU ml-1) of fishduring the summer season (p<0.05). The results of the study indicated that there is a risk of occurrence of disease outbreak under stressful situations. Moreover, the recovery of these bacteria in the fish which are potentially pathogenic to humans, suggests that if the fish are improperly handled, undercooked or consumed raw, may cause diseases to susceptible individuals.
Keywords:Bacterial pathogens, Lake Hawassa, Oriochromis niloticus, Prevalence
Economic appraisal of Indian marine products exports – A decadal analysis for the period 2001 to 2021
Authors: Jeevitha G. N. , Murari Singh Krishna , Ahmad Nasim , Srivastava Prem Prakash 
Published: February 2024

Fishing is an age-old practice and the fisheries sector is vital to India’s economy, whichcontributes to the primary livelihoods of about 2.8 crores of fishers and many more along thefisheries value chain. Fish and fish products constitute about 17% of India’s total agriculturalexports. The sector has been reckoned as a promising contributor to foreign exchangeearnings. The compound annual growth rates of exports and their instability from 2001 to2021 have been worked out for crustaceans, molluscs and frozen fish. The study revealedthat the quantity of crustacean exports has grown at a comparatively high and significantrate of 11.08% during the decade spanning 2011-2021 (Period-II) than during 2001-2010(Period-I). The growth in unit value realised from the export of molluscs declined from5.8% during Period-I to 2.8% during Period-II. The growth in unit value of frozen fish exportdeclined from 10.68% during Period-I to a slow growth rate of 0.11% in Period-II. The declinein the growth of unit values in molluscs and frozen fish indicates a declining demand and, inturn, a declining incentive for future exports from India.
Keywords:Export performance, Foreign exchange earnings, Growth, Instability, Marine products
Effect of stocking density on shooter formation, growth and survival of Asian seabass Lates calcarifer (Bloch, 1790) during nursery rearing
Authors: Solanki H. G. , Sumara M. A. K. , Vanza J. G. , Kailasam M. , Mahalakshmi P. , Kumar Prem , Ujjania N. C. 
Published: February 2024

Seabass Lates calcarifer is an important brackishwater fish, which thrives and farmed infresh and saline water environment. Differential growth of this species during larval andnursery rearing phases leads to the formation of shooters, which reduces overall survival dueto cannibalism. Therefore, the present experiment was conducted to evaluate the effect ofstocking density on shooter formation, growth and survival of seabass. Hatchery producedseabass seed (total mean length 1.76±0.39 cm; mean weight 0.132±0.10 g) were stocked innet cage hapa (2x1x1 m) installed in brackishwater pond, at four different stocking densities,viz., T1 (800 nos.), T2 (850 nos.), T3 (900 nos.) and T4 (950 nos.) in triplicates. During the45 days of nursery raising, fishes were fed with formulated nursery feed (crude protein: 42%and lipid 8%) at 5 to 8% of body weight three times a day. Initially, the fishes were manuallygraded on 3rd day and later on weekly basis and transferred to separate hapa. Total numberof shooters formed during grading periods (S1, S2, S3, S4 and S5) were counted. It wasobserved that though maximum shooters were obtained from treatment T1 and minimumin treatment T3, the shooter formation among different treatments were not significantlydifferent. The maximum numbers of shooters were obtained during the period from 9 to21 days of rearing in nurseries in all the treatments. A survival of 60% was recorded duringthe present study. Based on the results of this study, it is suggested to maintain stockingdensities of 400-425 m-2 for nursery rearing in hapa. Also, the rearing period from 9 to 21days is significantly critical for ensuring good survival rate.
Keywords:Cannibalism, Differential growth, Formulated feed, Nursery feed, Shooters, Stocking density, Survival rate
The process of spatial frequency information in temporal synchrony perception for audiovisual stimuli
Authors: Takeshima Yasuhiro 
Published: February 2024

Abstract
Previous studies have shown that the spatial frequency (SF) of visual stimuli alters the perceived timing of subjective simultaneity. However, these studies have been limited to the effects of a single SF component. In this study, I measured and compared the points of subjective simultaneity (PSS) for audiovisual stimuli among low, high, and composited SF components. This experiment comprised a dual-presentation timing task and a ternary response format to eliminate response bias for simultaneity judgment. The results indicated that the PSS value of the composition-SF stimuli was more toward visual-lead timing than the low-SF stimuli and did not differ significantly from that of the high-SF stimuli. The correlation coefficients showed that the PSS in composition-SF stimuli marginally approximated that of high-SF stimuli higher than that of low-SF stimuli. Future studies are needed to confirm these findings using visual stimuli with a wider range of SF components.
Combined treatment with Purpureocillum lilacinum MTL01 and Bacillus velezensis 83 reduces galling by Meloidogyne enterolobii and improves flowering in greenhouse-grown tomato
Authors: Lugo-García Luz Elena , Martínez-Anaya Claudia , Salinas-Castro Alejandro , Landa-Cadena Mahatma Gandhi , Vázquez Irán Tapia 
Published: February 2024

Abstract
M. enterolobii is an emergent phytopathogenic nematode that infects and reduces the yields of commercially important vegetables, such as tomatoes (Solanum lycopersicum). In Mexico, M. enterolobii has gained access to states previously unknown to be affected by it, especially northern states such as Sinaloa, which is the main producer of tomato in the country. Formulations based on antagonistic microorganisms exist in the Mexican market and are used in combination with chemically synthesized pesticides, which although effective are also unspecific and even toxic to other animals and humans. The ascomycetes Purpureocillum lilacinum (previously, Paecilomyces lilacinus) is an effective nematicide already used in different commercial formulations. Here, we aimed to determine the combined effect of an environmental strain of P. lilacinum MTL01 that we isolated, with spores of B. velezensis 83 that is also a growth promoter of tomato. Our findings of two assays in greenhouse conditions of tomato development treated with MTL01 and B. velezensis 83, either individually or in combination, and then challenged with stage-2 juveniles (J2) of M. enterolobii, indicate that the combined treatment is useful for reducing galling and stimulating flowering, with the potential of better yields to produce greenhouse-grown tomato.
Evaluation of the properties of Inconel® 625 preforms manufactured using WAAM technology
Authors: Silva Wemerson Felipe , Junior Givan Martins Macedo , Oliveira Hellen , Pires Julio Cezar Alvarenga , Carneiro Jose Rubens Gonçalves 
Published: February 2024

Abstract

Additive manufacturing is a constantly evolving technology that makes possible to manufacture highly complex parts by adding material layer by layer, as opposed to the various conventional manufacturing methods. Among additive manufacturing technologies, the Wire Arc Additive Manufacturing (WAAM) process stands out for its ability to manufacture large components with high mechanical properties and is a candidate to replace components manufactured by processes such as casting and forging. In order to increase the maturity of the process and understand the limits of the technology, this work objective is to evaluate the mechanical and metallurgical properties of a preform manufactured by the WAAM process on a substrate of the same alloy, comparing them with the reference material. To this end, the WAAM process was used to manufacture an Inconel® 625 preform, using wires as the deposition raw material and a substrate of the same alloy supplied forged. In order to select the processing window for the material, a Design of Experiment (DOE) was carried out, using deposition parameters as factors, such as: welding speed, stick-out, wire feed speed, shielding gas type and flow rate, among others, with the response of the experiment (DOE) being the geometric variables of the deposited layers (layer height and width) and the thermal input generated. After processing the information and selecting the processing window, four preforms were manufactured, following the same parameterization. After this, representative samples were taken to characterize the mechanical properties achieved and evaluate the metallurgical behaviour. Samples were prepared for macro and micrographic analysis through an optical microscope, a scanning electron microscope, mechanical hardness tests and tensile test specimens in three directions: vertical, horizontal and at the intersection with the substrate. After carrying out the mechanical tests on the preform and the substrate, the differences in mechanical properties were evaluated, with the sample manufactured by WAAM caracterizing lower strength and tensile limits than the forged Inconel® 625 substrate, however, with greater elongation when compared to the same forged material. The presence of solidification dendrites with porosity in the interdendritic regions and niobium microsegregation in the interdendritic regions were also observed. One hypothesis for the observations in this work is associated with anisotropy arising from the multiple thermal cycles that occur in the process, making it possible to obtain better properties after carrying out a heat treatment compatible with the microstructure of interest.
Relationship Between Leadership Behaviour and Firm Performance in Ready Made Garments (Rmg) Industry of Bangladesh
Authors: Tareque Alam , Islam Prof. Nazrul 
Published: February 2024

Abstract
Purpose
The primary objective of this research is to investigate and elaborate on the relationship between leadership behaviour and firm performance in the context of Bangladesh RMG (Ready-Made Garments) firms. The study aimed to contribute to the existing literature by exploring the role of leadership behaviour in the competitive advantage process, addressing gaps in understanding within the RMG industry.
Design/Methodology/Approach:
The research employs a mixed-method approach, incorporating qualitative and quantitative research methods. The conceptual model is developed through an extensive literature review and subsequently tested, refined, and validated. The empirical analysis involves 400 target respondents, and data is processed using PLS structural equation modelling principles. This includes structural path analysis, hypothesis testing, mediation, and moderation analyses.
Findings:
Empirical findings align with the resource-based view, confirming that Leadership Behaviours are intangible resources. Furthermore, the study identifies relationship-oriented behaviour as the most significant influence on the relationship between leadership behaviour and firm performance.
Originality:
This research delves into the Resource View's black box in firms. It establishes Dynamic Capabilities as a theoretically well-founded and valuable construct for understanding the leadership behaviour of strategic management.
Practical Implications:
providing insights into how leadership behaviour can impact firm performance. Identifying Relation Behaviours as particularly influential suggests that fostering positive relationships within the organisation may be a key strategy for leaders to enhance overall performance.
Social Implications:
the findings may have implications for workplace relationships, employee satisfaction, and potentially broader societal aspects influenced by the performance of RMG firms.
Vaginal Length Measurement and Examination of Sexual Functions in Patients Who Had Total or Subtotal Hysterectomy for Benign Reasons
Authors: kartal Duygu ucar , Dogan Ozan 
Published: February 2024

Abstract
Objective: It was aimed to evaluate the vaginal length in patients who underwent total or subtotal hysterectomy and to reveal its effects on the sexual functions of the patients depending on the choice of surgery.
Materials and Methods: 85 patients who were decided to undergo hysterectomy for benign reasons were included in the study. Vaginal lengths of all patients and cervical lengths of patients who underwent subtotal hysterectomy were recorded before and after the operation. In addition, the Female Sexual Function Index (FSFI) questionnaire was administered to the patients before and 24 months after the operation, and the International Index of Erectile Function (IIEF) questionnaire was administered to their spouses.
Results: While there was no significant change in the vaginal length of women who underwent subtotal hysterectomy after the operation (p>0.05), a significant shortening was detected in the total hysterectomy group (p:<0.0001). There was a decrease in the total FSFI scores of the patients after the operation in both groups (p<0.0001). However, the decrease in FSFI scores in the subtotal hysterectomy group was significantly less than that in the total hysterectomy group.
Conclusion: The decreasing FSFI scores of the patients after the operation show that both operations have negative effects on sexual functions. However, due to the vaginal tissue loss seen in the total hysterectomy group, the sexual functions of the patients were more negatively affected than the subtotal hysterectomy group.
Effectiveness of food quality and safety management systems in Oman’s food supply chain
Authors: Masengu Reason , Din Mohamed El , Ruzive Benson , Habsi Jouhara Al 
Published: February 2024

Abstract

This study has offered an extensive analysis of the effectiveness of Oman’s food supply chain’s quality and safety management. The study focused on the complex relationships among several variables, such as information technology, food traceability, supply chain risks, performance measures, and food safety regulations, and how these elements all work together to shape the efficiency of the food supply chain. The research examined data gathered from middle- and top-level managers in Oman’s food supply chain using a practical research strategy that combined quantitative and qualitative approaches and employed structural equation modelling to investigate the interconnections and effects on the performance of the supply chain from the previously listed factors. The key findings of this research have demonstrated the vital significance of strict food safety measures by revealing a substantial positive association between food safety standards and the performance of the food supply chain. Information technology has been found to be a significant catalyst for improving regulatory compliance and supply chain performance. The mediation study has highlighted how information technology is interdependent with other elements and demonstrated how it supports the robustness, agility and resilience of supply chains. This study further established that improving food safety standards, utilising cutting-edge information technology to enhance compliance and traceability, implementing thorough risk management plans, encouraging cooperation among supply chain participants and raising consumer knowledge of food safety are important suggestions to improve public health and economic growth. These suggestions are meant to assist Oman’s food supply chain in being robust, effective and safe. The research provided valuable insights for supply chain professionals, policymakers, and researchers who are interested in food safety and supply chain management, especially in the context of Oman and other GCC countries.
KAT2A-targeted inhibition prevents an inflammatory response to protect the myocardium
Authors: Lv Bin , Wang Xiaoying , Yu Haiyang , Chen Yang , Guo Jinhe , Du Yaodong , Feng Chao , Cui Rutao , Gao Xiumei 
Published: February 2024

Abstract
Background: Hyperactive inflammation is crucial for the development of acute myocardial infarction (AMI) to heart failure and can promote impaired cardiac function, and it is difficult to determine its specific therapeutic targets and drugs. Therefore, it is necessary to develop new therapeutic strategies to control inflammatory responses to protect myocardial cells during AMI.
Methods: Levels of critical cardiac markers and inflammatory factors were measured in blood samples collected from clinical patients with AMI to analyze the association between the serum expression levels of inflammatory factors and the progression of AMI. To uncover the mechanism underlying the association between inflammatory response and AMI, microarray analysis was performed to identify key myocardial cell regulators. ATAC-seq was also performed to identify how immune response genes are transcriptionally regulated by KAT2A. KAT2A-/- genetically engineered mice were used to validate the screened targets. Finally, a small molecule targeting KAT2A was developed and tested in a rat model of AMI.
Results: Here we show that KAT2A, a histone H3K9 acetyltransferase, is a key factor regulating the inflammatory response in AMI. Specifically, the level of cardiac damage was initially found to be associated with the inflammatory response in patients with AMI. Regarding the mechanism, KAT2A transcriptionally regulates pro-inflammatory tumor necrosis factor alpha and interleukin-6 to induced cardiomyocyte damage. Using a genetically engineered mouse model of AMI, we show that targeted KAT2A loss or inhibition prevents the recruitment of immune cells into the myocardium to suppress the inflammatory explosion in AMI in vivo and significantly improved heart function. Finally, Tanshinone I, a KAT2A-targeted inhibitor, could inhibited the inflammatory response in AMI in vivo.
Conclusion: KAT2A is a potential therapeutic target for AMI, especially for inhibiting the inflammatory response to this condition. As a specific KAT2A-targeted inhibitor, Tanshinone I is a potent candidate drug for AMI.
Dual-trigger release of berberine chloride from the Gelatin/Perfluorohexane core-shell structure
Authors: Givarian Mahshid , Moztarzadeh Fathollah , Ghaffari Maryam , Bahmanpour AmirHossein , Mollazadeh-Bajestani Maryam , Mokhtari-Dizaji Manijhe , Mehradnia Fatemeh 
Published: February 2024

Abstract

The development of smart nanocarriers that enable controlled drug release in response to internal and external triggers is an emerging approach for targeted therapy. This study focused on designing pH-sensitive, ultrasound-responsive gelatin/perfluorohexane (PFH) nanodroplets loaded with berberine chloride as a model drug. The nanodroplets were prepared using an emulsion technique and optimized by varying process parameters like homogenization rate, polymer concentration, surfactant, drug, and perfluorocarbon content. The optimal formulation yielded nanodroplets with a particle size of 281.7 nm, a drug encapsulation efficiency of 66.8 ± 1.7, and a passive drug release of 15.4 ± 0.2 within 24 hours. Characterization confirmed successful encapsulation and pH-responsive behavior. Ultrasound stimulation significantly enhanced drug release, with 150 kHz being more effective than 1 MHz in triggering acoustic droplet vaporization while minimizing heat generation. After 10 minutes of radiation, the optimal formulation showed 89.4% cumulative drug release. The nanodroplets displayed stability over one month at 4°C. Overall, the dual-triggered nanodroplets demonstrate excellent potential for controlled delivery and targeted release of berberine chloride.
Expression of Lipid Metabolism Genes Is Correlated With Immune Microenvironment and Predicts Prognosis in Endometrial Carcinoma
Authors: Chen Huang , Chen Ye , Liu Xiaoli , Weng Ling , Zeng Yongping , Wang Yanying , Zhao Lijuan 
Published: February 2024

Abstract
Objective
Endometrial carcinoma (EC) is one of the most prevalent types of gynecologic cancer. The purpose of this work was to identify the metabolic-related biological characteristics of endometrial cancer and to investigate the immune-related molecular pathways of carcinogenesis in endometrial cancer.
Methods
Data from The Cancer Genome Atlas (TCGA) were utilized to identify lipid metabolism-related genes (LMRGs) with significant correlations to the prognosis of EC patients. Enrichment of functional pathways within the LMRGs was studied. LASSO and Cox regression analysis were conducted to identify LMRGs that were significantly associated with the prognosis of EC patients. We created a prognostic signature and proved its effectiveness in both training and validation groups. In addition, we constructed a complete nomogram consisting of risk models and clinical variables to estimate the survival probability of EC patients.
Results
ACOT11, CYP1A2, GDPD5, MOGAT3, OLAH, PIASS4, PIP5K1C, PLPP2, and SRD5A1 were discovered to be strongly associated with the clinical outcomes of EC patients. On the basis of these nine LMRGs, we generated and validated our predictive signature using the training and validation cohorts. In addition to being independent of other clinical factors, the nine-LMRG signature distinguished between patients at high- and low-risk for EC and predict EC patient's probability of survival. Statistically, the nomogram exhibited a high correlation between survival forecasts and observations. In the high-risk group, immune/stromal scores were lower and there was a higher density of several kinds of immune cells.
Conclusions
The LMRG's prognostic model and comprehensive nomogram could guide therapeutic choices in clinical practice, and explore the underlying mechanisms involved in EC progression.