bluuebunny/crossref_metadata_2025_split huggingface dataset:
Renewable energy consumption and business density as drivers of sustainable development
Authors: Zheng Renzhi , Osabohien Romanus , Madueke Emmanuel , Jaaffar Amar Hisham Bin 
Published: October 2023

The East Asia and Pacific region have witnessed significant economic growth in recent decades, leading to improved living standards and increased energy consumption. However, this rapid development has also resulted in environmental degradation and heightened concerns about climate change. In response, countries in the region have been increasingly focusing on sustainable development, with renewable energy consumption and business density emerging as key drivers. Given this background, this research empirically examines the impact of entrepreneurship and clean technology on sustainable development in East Asia and the Pacific region. To control for endogeneity, the study applied system the Generalized Method of Moments (SGMM) for the data sourced from the World Development Indicators of the World Bank for the period 2015–2021. The finding indicates that both entrepreneurship and clean technology have an adverse significant impact on sustainable development in the East Asia-Pacific region. When the regions were analysed individually, the results revealed that both entrepreneurship and clean technology have a negative substantial effect on sustainable development in East Asia, but not in the Pacific region. The study concludes that entrepreneurship and renewable energy by themselves would not result in sustainable development, except some certain conditions are met. The positive impacts of entrepreneurship and renewable energy on sustainable development can only be attained when the nature, location, and practices of new businesses are low carbon in nature.JEL Classifications: Q01, Q42, Q56
Respiration, phytoplankton size and the metabolic balance in the Atlantic gyres
Authors: Serret Pablo , Lozano Jose , Harris Carolyn B. , Lange Priscila K. , Tarran Glen A. , Tilstone Gavin H. , Woodward E. Malcolm S. , Zubkov Mikhail V. 
Published: October 2023

The balance between plankton photosynthesis (GPP) and community respiration (CR) in the euphotic zone (net community production, NCP) is an essential driver of the biological carbon pump. Deficient datasets and a lack of knowledge of the mechanisms regulating CR cause poor empirical models and oversimplified parameterisations that maintain NCP as one of the most important unknowns for projections of the carbon pump. One important unresolved issue is the unexpected lack of empirical relationships between CR and the biomass or size-structure of the phytoplankton, which undermines the use of remotely sensed observations to predict net community metabolism. Here we analyse the spatial variation of plankton metabolism, chlorophyll a concentration (Chla), pico- and nanophytoplankton abundance and size-fractionated primary production (14CPP) along a latitudinal (49°N–46°S) transect of 73 stations across the Atlantic Ocean (AMT-22 cruise). The use of depth-weighted rates (rates integrated to the depth of 0.1% PAR, divided by the regionally varying depth of integration) markedly improved the depiction of latitudinal patterns and the significance of relationships, over volumetric or integrated rates. Depth-weighted CR showed clear and consistent latitudinal patterns with relevance for the distribution of NCP. Depth-weighted Chla and CR exhibited a significant relationship (CRZ=1.42ChlaZ-0.21, r2 = 0.69, N=37, p&lt;0.001) with potential for the difficult prediction of CR. A general ratio of 1.42 mmolO2 mgChla-1 d-1 and a threshold Chla for net heterotrophy of ca. 0.25 mgChla m-3 can be tentatively proposed for the Atlantic, although further analyses of spatial and seasonal variation are necessary. We observed unusually positive NCP rates in the central part of the N gyre, due to a marked decrease of CR in a patch of high Synechococcus spp. abundance and high 14CPP by large phytoplankton. However, no relationship was observed between size-fractionated 14CPP and CR or the GPP : CR ratio during the cruise, contradicting the hypothesis that food web functioning is determined by the phytoplankton size structure. Such independence, together with the persistence of distinct GPP : CR and 14CPP : NCP relationships in distinct biogeographic provinces suggest a resilience of trophic dynamics and the existence of alternative ecosystem states, whose implications for projections of the metabolic state of the ocean are discussed.
Improving data reliability to support marine pollution assessment according to MSFD Descriptor 8 in the European Seas: the contribution of EMODnet Chemistry
Authors: French Megan Anne , Lipizer Marina 
Published: October 2023

IntroductionAccording to the Marine Strategy Framework Directive (MSFD, 2008/56/EC), member states of the European Union (EU) had to develop a common approach in environmental monitoring and assessment. Regarding marine pollution assessments, large heterogeneities remain regarding sampling protocols, analytical methods, and quality assurance (QA) and quality control (QC) procedures. Further, data availability for Descriptor 8 (contaminants) was very fragmented in the first cycle of the MSFD. As one of the major EU spatial data infrastructures for providing access to marine data, EMODnet Chemistry has endeavoured to overcome data fragmentation and increase data ‘FAIRness’ (findable, accessible, interoperable, and reusable).MethodsAiming to improve the reliability of marine contaminant data for assessment purposes under the MSFD, detailed QA/QC information was collected using a questionnaire based on the requirements of the European Environment Agency, International Council for the Exploration of the Sea, United Nations Environment Program–Mediterranean Action Plan, and NORMAN Network. The questionnaire was distributed to institutions in 26 countries participating in EMODnet Chemistry.ResultsInformation was received from 18 countries on ~90% of the substances included in EU legislation on priority substances. The results indicate an overall good level of laboratory proficiency; however, heterogeneities were observed in sampling protocols (especially for biota) and analytical methods (e.g. for metals), suggesting the potential for increased harmonisation. While laboratory proficiency and equipment depend on institutional resources that may differ among countries, it is fundamental to share detailed QA/QC information associated with data to improve data reliability and re-usability, and to support marine pollution assessment.DiscussionEnriching data with required metadata and detailed QA/QC is part of the data curation process, which is still an overlooked aspect of the overall scientific research process and is crucial to support a reliable assessment of marine pollution, and ultimately better management of the marine environment.
Identification ferroptosis-related hub genes and diagnostic model in Alzheimer’s disease
Authors: Zhao Huabin , Wang Jiawei , Li Zhongzheng , Wang Shenghui , Yu Guoying , Wang Lan 
Published: October 2023

BackgroundFerroptosis is a newly defined form of programmed cell death and plays an important role in Alzheimer’s disease (AD) pathology. This study aimed to integrate bioinformatics techniques to explore biomarkers to support the correlation between ferroptosis and AD. In addition, further investigation of ferroptosis-related biomarkers was conducted on the transcriptome characteristics in the asymptomatic AD (AsymAD).MethodsThe microarray datasets GSE118553, GSE132903, GSE33000, and GSE157239 on AD were downloaded from the GEO database. The list of ferroptosis-related genes was extracted from the FerrDb website. Differentially expressed genes (DEGs) were identified by R “limma” package and used to screen ferroptosis-related hub genes. The random forest algorithm was used to construct the diagnostic model through hub genes. The immune cell infiltration was also analyzed by CIBERSORTx. The miRNet and DGIdb database were used to identify microRNAs (miRNAs) and drugs which targeting hub genes.ResultsWe identified 18 ferroptosis-related hub genes anomalously expressed in AD, and consistent expression trends had been observed in both AsymAD The random forest diagnosis model had good prediction results in both training set (AUC = 0.824) and validation set (AUC = 0.734). Immune cell infiltration was analyzed and the results showed that CD4+ T cells resting memory, macrophages M2 and neutrophils were significantly higher in AD. A significant correlation of hub genes with immune infiltration was observed, such as DDIT4 showed strong positive correlation with CD4+ T cells memory resting and AKR1C2 had positive correlation with Macrophages M2. Additionally, the microRNAs (miRNAs) and drugs which targeting hub genes were screened.ConclusionThese results suggest that ferroptosis-related hub genes we screened played a part in the pathological progression of AD. We explored the potential of these genes as diagnostic markers and their relevance to immune cells which will help in understanding the development of AD. Targeting miRNAs and drugs provides new research clues for preventing the development of AD.
Retrospective analysis of outcomes for pediatric acute lymphoblastic leukemia in South American centers
Authors: Duffy Caitlyn , Graetz Dylan E. , Lopez Arturo M. Zapata , Carrillo Angela K. , Job Godwin , Chen Yichen , Devidas Meenakshi , Leon Sandra Alarcón , Bonzi Sol Aponte , Flores Pedro Cardona , Torres Lizeth Escobar , Broncano Eddy Hernández , Jaramillo Soledad Jiménez , Zelada Ma Ofelia , Novoa Romulo Reaño , Samudio Angelica , Sánchez-Fernandez Gissela , Villanueva Erika , Metzger Monika L. , Friedrich Paola , Jeha Sima 
Published: October 2023

IntroductionAcute lymphoblastic Leukemia (ALL) is the most common pediatric malignancy. While the survival rate for childhood ALL exceeds 90% in high-income countries, the estimated survival in low-and middle-income countries ranges from 22-79%, depending on the region and local resources.MethodsThis study retrospectively reviewed demographic, biological, and clinical parameters of children under 18 years of age with newly diagnosed ALL presenting between 2013-2017 across five pediatric centers in 4 countries in South America. Survival analyses were estimated using the Kaplan-Meier method.ResultsAcross the five centers, 752 patients were analyzed (Bolivia [N=9], Ecuador [N=221], Paraguay [N=197], Peru [N=325]) and 92.1% (n=690) patients were diagnosed with B-cell and 7.5% (n= 56) with T-cell ALL. The median age was 5.5 years old (IQR 7.29). At diagnosis, 47.8% of patients were categorized as standard and 51.9% as high risk per their institutional regimen. Advanced diagnostics availability varied between modalities. MRD was evaluated in 69.1% of patients; molecular testing was available for ETV6-RUNX, BCR-ABL1, TCF3-PBX1, and KMT2A-rearranged ALL in 75-81% of patients; however, karyotyping and evaluation for iAMP21 were only performed in 42-61% of patients. Central nervous system (CNS) involvement was evaluated at diagnosis in 57.3% (n=429) patients; of these, 93.7% (n=402) were CNS 1, 1.6% (n=7) were CNS 2, 0.7% (n=11) were CNS3, 1.9% (n=8) had cranial nerve palsy, and 2.1% (n=9) results unavailable. Chemotherapy delays &gt;2 weeks were reported in 56.0% (n=421) patients during treatment. Delays were attributed to infection in 63.2% (n=265), drug-related toxicities in 47.3% (n=198), and resource constraints, including lack of bed availability in 23.2% (n=97) of patients. The 3-year Abandonment-sensitive EFS and OS were 61.0±1.9% and 67.2±1.8%, respectively. The 3-year EFS and OS were 71.0±1.8% and 79.6±1.7%, respectively.DiscussionThis work reveals opportunities to improve survival, including addressing severe infections, treatment interruptions, and modifications due to drug shortages. In 2018, healthcare professionals across South America established the Pediatric Oncology Latin America (POLA) group in collaboration with St. Jude Children’s Research Hospital. POLA collaborators developed an evidence-based, consensus-derived, adapted treatment guideline, informed by preliminary results of this evaluation, to serve as the new standard of care for pediatric ALL in participating institutions.
Cloning and functional analysis of the BrCUC2 gene in Brassica rapa L
Authors: Tao Xiaolei , Zhao Yuhong , Ma Li , Wu Junyan , Zeng Rui , Jiao JinTang , Li Rong , Ma Weiming , Lian Yintao , Wang Wangtian , Pu Yuanyuan , Yang Gang , Liu Lijun , Li Xuecai , Sun Wancang 
Published: October 2023

The CUP-SHAPED COTYLEDON2 (CUC2) gene plays an important role in the formation of apical meristem and organ edges in plants. The apical meristematic tissue of Brassica rapa (B. rapa) is associated with cold resistance, however, the role of the CUC2 gene in cold resistance of B.rapa is unclear. In this study, we used bioinformatics software to analyze the structure of BrCUC2 gene, real-time fluorescence quantitative PCR to detect the expression level of BrCUC2, constructed transgenic Arabidopsis thaliana by the flower dipping method and subcellular localization for functional validation. The results showed that, we isolated a 1104 bp open reading frame of BrCUC2 from the winter B. rapa cultivar ‘Longyou 7’. The BrCUC2 contains a highly conserved domain belonging to the NAM superfamily. Its homologus CUC genes contain similar conserved motifs and are closely related to Brassica oleracea (B.oleracea), and the N-terminal of amino acid sequence contains NAC domain. BrCUC2 protein was localized in the nucleus and self-activation tests showed that pGBKT7-BrCUC2 had self-activation. Tissue-specific expression analysis and promoter β-Glucuronidase (GUS) activity showed that BrCUC2 had high expression levels in B. rapa growth points and A. thaliana leaf edges, stems and growth points. After low-temperature stress, BrCUC2 showed greater expression in ‘Longyou 7,’ which presents strong cold resistance and concave growth points, than in ‘Longyou 99,’ which presents weak cold resistance and protruding growth points. BrCUC2 promoter contains multiple elements related to stress responses. BrCUC2 overexpression revealed that the phenotype did not differ from that of the wild type during the seedling stage but showed weak growth and a dwarf phenotype during the flowering and mature stages. After low-temperature treatment, the physiological indexes and survival rate of BrCUC2-overexpression lines of Arabidopsis thaliana (A. thaliana) were better than those of the wild type within 12 h, although differences were not observed after 24 h. These results showed that BrCUC2 improved the low-temperature tolerance of transgenic A. thaliana within a short time. It can provide a foundation for the study of cold resistance in winter B. rapa.
Comprehensive assessment of executive functioning following childhood severe traumatic brain injury: clinical utility of the child executive functions battery
Authors: Chevignard Mathilde , Guerra Amanda , Fliss Rafika , Salah Lucie , Pineau Emmanuelle , Notteghem Pauline , Roche Jeanne , Huon Caroline , Krasny Pacini Agata , Le Gall Didier , Fournet Nathalie , Roulin Jean-Luc , Roy Arnaud 
Published: October 2023

ObjectivesTo perform a detailed description of executive functioning following moderate-to-severe childhood traumatic brain injury (TBI), and to study demographic and severity factors influencing outcome.MethodsA convenience sample of children/adolescents aged 7–16 years, referred to a rehabilitation department after a TBI (n= 43), was compared to normative data using a newly developed neuropsychological test battery (Child Executive Functions Battery—CEF-B) and the BRIEF.ResultsPerformance in the TBI group was significantly impaired in most of the CEF-B subtests, with moderate to large effect sizes. Regarding everyday life, patients were significantly impaired in most BRIEF clinical scales, either in parent or in teacher reports. Univariate correlations in the TBI group did not yield significant correlations between the CEF-B and socio-economic status, TBI severity, age at injury, or time since injury.ConclusionExecutive functioning is severely altered following moderate-to-severe childhood TBI and is best assessed using a combination of developmentally appropriate neuropsychological tests and behavioral ratings to provide a comprehensive understanding of children’s executive functions.
Influence of servant leadership on the life satisfaction of basic education teachers: the mediating role of satisfaction with job resources
Authors: Quinteros-Durand Ronald , Almanza-Cabe Robinson B. , Morales-García Wilter C. , Mamani-Benito Oscar , Sairitupa-Sanchez Liset Z. , Puño-Quispe Lucy , Saintila Jacksaint , Saavedra-Sandoval Renán , Paredes Alcides Flores , Ramírez-Coronel Andrés Alexis 
Published: October 2023

BackgroundLife satisfaction is essential for teachers’ work performance and student learning. Additionally, servant leadership has been shown to be one of the best leadership practices as it promotes employee well-being and satisfaction. Moreover, satisfaction with job resources acts as a mediator in the relationship between servant leadership and life satisfaction by influencing individual and collective performance in the organization.ObjectiveThis research aimed to evaluate the mediating role of satisfaction with job resources in the relationship between servant leadership and life satisfaction.MethodsThe study was cross-sectional and explanatory. 620 teachers aged between 20 and 62 years (M = 35 and SD = 9.49) participated in the study. Structural Equation Modeling (SEM) was used to measure life satisfaction, service leadership, and job resource satisfaction through the use of questionnaires.ResultsThe results indicated that the model obtained an adequate fit, χ2 = 2,658, df = 551, p &lt; 0.001, CFI = 0.941, TLI = 0.936, RMSEA = 0.079, SRMR = 0.070. The results confirm the positive influence of leadership on satisfaction with resources and life satisfaction. Additionally, a positive influence of satisfaction with job resources on life satisfaction was observed. Moreover, the mediation of job resources in servant leadership and life satisfaction was confirmed.ConclusionServant leadership, supported by satisfaction with job resources, can reduce effort and associated costs, stimulate personal growth and learning, and improve the well-being of teachers.
Highly sensitised individuals present a distinct Treg signature compared to unsensitised individuals on haemodialysis
Authors: Dudreuilh C. , Basu S. , Shaw O. , Burton H. , Mamode N. , Harris F. , Tree T. , Nedyalko P. , Terranova-Barberio M. , Lombardi G. , Scottà C. , Dorling A. 
Published: October 2023

IntroductionHighly sensitised (HS) patients represent up to 30% of patients on the kidney transplant waiting list. When they are transplanted, they have a high risk of acute/chronic rejection and long-term allograft loss. Regulatory T cells (Tregs) (CD4+CD25hiCD127lo) are T cells involved in the suppression of immune alloresponses. A particular subset, called T follicular regulatory T cells (Tfr, CXCR5+Bcl-6+), is involved in regulating interactions between T effectors and B cells within the germinal centre and can be found in peripheral blood. Therefore, we wanted to identify specific subsets of Tregs in the peripheral blood of HS individuals.MethodsWe recruited prospectively healthy volunteers (HV) (n = 9), non-sensitised patients on haemodialysis (HD) (n = 9) and HS individuals, all of whom were on haemodialysis (n = 15).ResultsWe compared the Treg phenotypes of HV, HD and HS. HS patients had more CD161+ Tregs (p = 0.02) and more CD45RA−CCR7− T effectors (Teffs) (p = 0.04, memory Teffs able to home to the germinal centre) compared to HVs. HS patients had more Bcl-6+ Tregs (p &lt; 0.05), fewer Th1-like Tregs, more Th2-like Tregs (p &lt; 0.001) and more CD161+ (p &lt; 0.05) Tregs compared to HD patients. This population has been described to be highly suppressive. HD had a deficiency in a Th17-like CD161+ effector Treg cluster (cluster iii., CCR6+CCR4+CXCR3− CD39+CD15s+ICOS−CCR7−CD161+) (p &lt; 0.05).DiscussionThis is the first study presenting a deep Treg phenotype in HS patients. We confirmed that HS patients had more of a Th17-like CD161+ effector Treg from population III (CD4+CD25hiCD127loCD45RA−) compared to non-sensitised patients on HD. The clinical relevance of this highly suppressive Tregs population remains to be determined in the context of transplantation.
Use of immune checkpoint inhibitors in solid organ transplant recipients with advanced cutaneous malignancies
Authors: Ji Stephanie , Liu Hao , Pachella Laura , Stephenson Ryan D. , Groisberg Roman , Weiss Sarah A. 
Published: October 2023

BackgroundImmune checkpoint inhibitors (ICI) are standard of care therapy for patients with cutaneous malignancies, the most frequently diagnosed cancers in solid organ transplant (SOT) recipients. The activity and rate of allograft rejection in SOT recipients with advanced skin cancers treated with ICI is understudied.MethodsWe conducted a retrospective analysis of SOT recipients with advanced melanoma, cutaneous squamous cell carcinoma (cSCC), and merkel cell carcinoma (MCC) who were treated with ICI. Unpublished cases from our institution and published cases from the literature were aggregated. Demographics, type of immunosuppressive therapy, type of ICI(s) administered, prior systemic therapies, tumor response to ICI, and evidence of organ rejection and/or failure were recorded. Objective response rates (ORR) and rates of graft rejection and failure are reported.ResultsNinety patients were identified; four patients from our institution and 86 unique patients from a literature review. ORR to first-line ICI for the entire cohort was 41.1% (37/90). ORR by tumor type was 31% (18/58), 64.3% (18/28), and 25.0% (1/4) for melanoma, cSCC, and MCC, respectively. The rate of graft rejection was 37.8% (34/90) with 61.8% (21/34) of these cases progressing to graft failure. Number of immunosuppressive agents (0, 1, 2, or 3) was inversely associated with rate of graft failure.ConclusionsIn this retrospective analysis, ICIs demonstrate clinical activity in SOT recipients with cutaneous malignancies; however, the rate of graft rejection is high. Treatment plans should be individualized through thorough interdisciplinary discussion. Immunosuppressive modifications may be considered prior to starting treatment, but when feasible, enrollment on clinical trials is preferred.