bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein disulfide isomerase A3 as novel biomarker for endometrial cancer
Authors: Yu Fanrong , Liu Xin , Li Min , Liu Xiufen , Wang Xintai , Guo Meixiang 
Published: October 2023

ObjectiveThis study aims to investigate the potential of PDIA3 as a novel prognostic biomarker and therapeutic target for Endometrial Cancer (EC) with the ultimate goal of improving survival rates in EC patients.MethodsThis study employed a combination of public database analysis and clinical tissue sample assays. The analysis included comparing the gene expression of PDIA3 between EC and adjacent paracancerous tissues, investigating this expression status using qPCR and immunohistochemistry (IHC) assays, studying the correlation of expression with different parameters using Chi-square test, Cox Regression, and log-rank test, as well as exploring the PDIA3-related immune infiltration and metabolic pathway using TIMER and GSEA.ResultsThe analysis of public datasets revealed that PDIA3 mRNA and protein expression was significantly higher in EC tissues compared to adjacent tissues (P = 4.1e-03, P = 1.95e-14, and P = 1.6e-27, respectively). The qPCR analysis supported this finding (P = 0.029). IHC analysis revealed a significant increase in PDIA3 expression in endometrial cancer (EC) tissues compared to adjacent normal tissues (P = 0.01). Furthermore, PDIA3 expression showed significant correlations with cancer stage and tumor grade. Multivariate Cox regression analysis suggested that the PDIA3 gene holds promise as a prognostic factor for EC patients (HR = 0.47, 95% CI [0.27, 0.82], P = 0.008). The results from TIMER demonstrated a positive correlation between PDIA3 and tumor-infiltrating CD8 T cells and macrophages, and a negative correlation with tumor-infiltrating CD4 T cells. Additionally, the GSEA results indicated that PDIA3 overexpression was associated with various metabolic processes in EC patients.ConclusionPDIA3 has been validated as a potential biomarker for EC, and its expression is further associated with pathological staging and prognosis.
Comprehensive multi-omics analysis of tryptophan metabolism-related gene expression signature to predict prognosis in gastric cancer
Authors: Luo Peng , Chen Guojun , Shi Zhaoqi , Yang Jin , Wang Xianfa , Pan Junhai , Zhu Linghua 
Published: October 2023

Introduction: The 5-year survival of gastric cancer (GC) patients with advanced stage remains poor. Some evidence has indicated that tryptophan metabolism may induce cancer progression through immunosuppressive responses and promote the malignancy of cancer cells. The role of tryptophan and its metabolism should be explored for an in-depth understanding of molecular mechanisms during GC development.Material and methods: We utilized the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) dataset to screen tryptophan metabolism-associated genes via single sample gene set enrichment analysis (ssGSEA) and correlation analysis. Consensus clustering analysis was employed to construct different molecular subtypes. Most common differentially expressed genes (DEGs) were determined from the molecular subtypes. Univariate cox analysis as well as lasso were performed to establish a tryptophan metabolism-associated gene signature. Gene Set Enrichment Analysis (GSEA) was utilized to evaluate signaling pathways. ESTIMATE, ssGSEA, and TIDE were used for the evaluation of the gastric tumor microenvironment.Results: Two tryptophan metabolism-associated gene molecular subtypes were constructed. Compared to the C2 subtype, the C1 subtype showed better prognosis with increased CD4 positive memory T cells as well as activated dendritic cells (DCs) infiltration and suppressed M2-phenotype macrophages inside the tumor microenvironment. The immune checkpoint was downregulated in the C1 subtype. A total of eight key genes, EFNA3, GPX3, RGS2, CXCR4, SGCE, ADH4, CST2, and GPC3, were screened for the establishment of a prognostic risk model.Conclusion: This study concluded that the tryptophan metabolism-associated genes can be applied in GC prognostic prediction. The risk model established in the current study was highly accurate in GC survival prediction.
Efficacy and safety of Panax notoginseng saponins (Xuesaitong) for patients with acute ischemic stroke: a systematic review and meta-analysis of randomized controlled trials
Authors: Shi Xinyi , Feng Luda , Li Yixuan , Qin Mingzhen , Li Tingting , Cheng Zixin , Zhang Xuebin , Zhou Congren , Cheng Sisong , Zhang Chi , Gao Ying 
Published: October 2023

Background: Stroke is the major cause of mortality and permanent disability and is associated with an astonishing economic burden worldwide. In the past few decades, accumulated evidence has indicated that Xuesaitong (XST) has therapeutic benefits in cases of acute ischemic stroke (AIS). Our study aimed to provide the best current body of evidence of the efficacy and safety of XST for patients with AIS.Methods: This is a systematic review and meta-analysis of randomized controlled trials (RCTs). We searched eight electronic databases from inception to 17 July 2023 for relevant RCTs. The investigators independently screened trials, extracted data, and assessed the risk of bias. A meta-analysis was conducted using RevMan 5.3 and STATA 16.0 software.Results: In total, 46 RCTs involving 7,957 patients were included. The results showed that XST improved the long-term functional outcomes with lower modified Rankin Scale (mRS) scores (MD = −0.67; 95% CI [−0.92 to −0.42]; p &lt; 0.00001) and a higher proportion of functional independence (mRS ≤2) (RR = 1.08; 95% CI [1.05 to 1.12]; p &lt; 0.00001). Low-quality evidence indicated that XST improved the activities of daily living (MD = 10.17; 95% CI [7.28 to 13.06]; p &lt; 0.00001), improved the neurological impairment (MD = −3.39; 95% CI [−3.94 to −2.84]; p &lt; 0.00001), and enhanced the total efficiency rate (RR = 1.19; 95% CI [1.15 to 1.23]; p &lt; 0.00001). No significant difference was found in the all-cause mortality or incidence of adverse events between the XST and control groups. The certainty of evidence was estimated as moderate to very low.Conclusion: Presently, the administration of XST within 14 days of AIS is associated with favorable long-term functional outcomes. In addition, XST can improve activities of daily living, alleviate neurological deficits, and has shown good tolerability. However, the current evidence is too weak, and the confidence of evidence synthesis was restricted by the high risk of bias. Given the insufficient evidence, appropriately sized and powered RCTs investigating the efficacy and safety of XST for patients with AIS are warranted.Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=446208, CRD42023446208.
Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell model
Authors: Jana Sirsendu , Kassa Tigist , Wood Francine , Hicks Wayne , Alayash Abdu I. 
Published: October 2023

Red blood cells (RBCs) undergo metabolic, oxidative, and physiological changes during storage, collectively described as the “storage lesion.” The impact of storage on oxygen homeostasis, following transfusion, is not fully understood. We show that RBC storage induces changes in oxygen binding that were linked to changes in oxygen sensing (hypoxia-inducible factor, HIF-1α) mechanisms and mitochondrial respiration in human pulmonary arterial endothelial cells (HPAECs). A decrease in oxygen affinity (P50) to approximately 20 from 30 mmHg was seen at the first week but remained unchanged for up to 42 days. This led to the suppression of HIF-1α in the first 3 weeks due to limited oxygen supplies by RBCs. Furthermore, membrane oxidative damage, band 3 alterations, and subsequent microparticle (MP) formation were also noted. Mass spectrometric analysis revealed the upregulation of transitional endoplasmic reticulum ATPase, essential for clearing ROS-damaged membrane proteins and the protein DDI1 homolog, a proteasomal shuttle chaperone. Band 3 complex proteins and superoxide dismutase were among the downregulated proteins. Mitochondrial oxygen consumption rates measured in HPAECs incubated with RBC-derived MPs (14-day and 42-day) showed a rise in maximal respiration. Intervention strategies that target intracellular hemoglobin (Hb)’s redox transitions and membrane changes may lead to the reestablishment of oxygen homeostasis in old RBCs.
FGF23 level in poultry chicken, a systematic review and meta-analysis
Authors: Poorhemati Hossein , Ghaly Melody , Sadvakassova Gulzhakhan , Komarova Svetlana V. 
Published: October 2023

Introduction: In vertebrates fibroblast growth factor 23 (FGF23) is a phosphate regulating hormone closely linked to calcium regulation by vitamin D and parathyroid hormone (PTH). Although phosphorus, calcium and vitamin D are important for poultry well-being, relatively little is known about their levels of FGF23. Our objective was to quantitatively estimate the blood FGF23 level in birds, and to examine its relationship to diet and blood levels of other components of phosphate and calcium homeostasis.Methods: A systematic search of Agricola, Embase and Medline identified 86 studies focused on FGF23 in birds, from which 12 manuscripts reporting data for 60 independent groups of chickens were included in the analysis.Results: FGF23 levels were 256 pg/ml (Confidence interval (CI): 215, 297) in broilers (39 datasets containing 435 birds), and 256 pg/ml (CI: 178, 339) in egg-laying hens (21 datasets containing 208 birds). FGF23 levels did not correlate with dietary phosphorus, calcium or vitamin D, or with plasma calcium or PTH. FGF23 levels demonstrated a trend to positively correlate with plasma phosphate and a strongly and positive correlation with plasma vitamin D.Discussion: This study provides normative estimates of FGF23 levels in poultry birds and new insights into the regulation of calcium and phosphate homeostasis.
Measuring mentalizing in youth: further validation of the reflective functioning questionnaire for youth (RFQY-13)
Authors: Martin-Gagnon Gabriel , Fonagy Peter , Bégin Michaël , Normandin Lina , Ensink Karin 
Published: October 2023

ObjectiveThis study investigated the psychometric properties, including the factor structure, validity, and reliability of the 13-item Reflective Function Questionnaire for Youth (RFQY-13), using a new scoring system.MethodA community sample of 414 adolescents and a clinical sample of 83 adolescents (aged 12–21) completed the RFQY, the Borderline Personality Features Scale for Children (BPFS-C), the Beck Youth Inventories (BYI), the Child Behavior Checklist-Youth Self Report (CBCL-YSR) and the Movie for the Assessment of Social Cognition (MASC).ResultsUsing the new scoring system, our results demonstrated configural and metric invariance, as well as adequate reliability and validity across both samples for the two-factor structure of the RFQY. The Uncertainty subscale also showed strong associations with psychopathology.DiscussionThe findings show that the RFQY-13, when used with the new coding system, has good psychometric properties and is a reliable measure of mentalizing for adolescents and young adults. We discuss clinical implications, limitations and future directions.
Spirulina and its residual biomass as alternative sustainable ingredients: impact on the rheological and nutritional features of wheat bread manufacture
Authors: Fratelli Camilly , Nunes Maria Cristiana , De Rosso Veridiana Vera , Raymundo Anabela , Braga Anna Rafaela Cavalcante 
Published: October 2023

Introduction: Following consumers’ demand for healthier alternatives, Spirulina biomass has been included as a functional ingredient in different types of food as a sustainable alternative to improve physicochemical characteristics and nutritional aspects in the diet. This study aimed to explore the use of Spirulina biomass (SB) and residual biomass (RB) obtained after C-phycocyanin (C-PC) extraction in the production of wheat bread (WB).Methods: The dough rheology, texture, color, nutritional aspects, and bioaccessibility of the different manufactured bread samples were accessed to achieve this goal.Results and Discussion: This is the first research to exploit RB. It was possible to replace 3% of wheat flour for SB or RB in bread-making; this substitution did not impact the rheological characteristics of the dough, even though the pH increased with the addition of SB or RB (5.63 and 5.70, respectively). SB and RB addition improved bread volume and enhanced the nutritional profile, increasing the antioxidant capacity (DPPH and FRAP) compared to WB. Heavy metals (Ni, Cd, and Pb) were not found in any of the samples (&lt;0.2 mg/100 g). The in vitro protein digestibility in bread was better than in SB and RB raw materials, which indicates that bread manufacturing may contribute to improving protein digestibility. In addition, SB needs greater appreciation for sustainable food practices worldwide and in Brazilian exploration, requiring management strategies with industry and society working together. Further studies are necessary, focusing on acceptability to understand the viability of these ingredients to target consumers’ preferences.
Sheath Blight of Maize: An Overview and Prospects for Future Research Directions
Authors: Di Runze , Liu Lun , Shoaib Noman , Xi Boai , Zhou Qiyan , Yu Guowu 
Published: October 2023

Sheath blight (ShB) of maize, as a soil-borne disease caused by Rhizoctonia solani AG1-IA, is one of the main obstacles for maintaining the sustainable production of maize. R. solani has a wide host range and low-resistance sources, there is a lack of resistant resources against ShB, and the damage caused by ShB cannot be effectively controlled. To effectively protect crops against ShB, it is necessary to combine information about its pathogenicity and about the disease prevention and control of the pathogenic fungus and to identify areas that require more in-depth research. While progress has been made in the identification of disease-related genes in corn and pathogens, their mechanisms remain unclear. Research related to disease control involves the use of agronomic methods, chemical control, biological control, and genetic improvement. Breeding and identification of high-resistant maize varieties are key and difficult points in the control of maize sheath blight. This article reviews the research progress of the symptoms, the pathogen’s biological characteristics, the infection process, the pathogenic mechanism, and comprehensive control of maize sheath blight disease and provides future research directions of maize sheath blight in China. It aims to provide reliable technical routes and research ideas for future crop-disease-resistance research, especially to sheath blight of maize.
Review of the Narrow-Banded Hawkmoth, Neogurelca montana (Rothschild & Jordan, 1915) (Lepidoptera: Sphingidae) in China, with Morphological and Phylogenetic Analysis
Authors: Xu Zhen-Bang , He Ji-Bai , Yang Nan , Kitching Ian J. , Hu Shao-Ji 
Published: October 2023

Neogurelca montana (Rothschild & Jordan, 1915) is a species of the genus Neogurelca Hogenes & Treadaway, 1993, that was previously known from Sichuan, Yunnan, and Tibet, China. Recently, however, this species was also found in Beijing and Hebei. These populations differ from those in southwest China in body colour and the shape of the yellow patches of the hindwing—a paler body colour and triangular patches in the former and darker body colour and fan-like patches in the latter. Wing morphology, male and female genitalia, and molecular evidence (DNA barcodes) were analysed for the different localities of this species and three other Neogurelca species—N. hyas, N. himachala, and N. masuriensis. Our molecular data support the Beijing population of montana as a valid subspecies, which we describe as N. montana taihangensis ssp. nov. Wing and genital morphology confirm the molecular conclusions. We also collected larvae of the new subspecies in the Beijing suburbs and describe its life history and larval hosts and compare them with those of N. himachala.
The Molecular Genetic Epidemiology and Antimicrobial Resistance of Neisseria gonorrhoeae Strains Obtained from Clinical Isolates in Central Panama
Authors: Núñez-Samudio Virginia , Herrera Mellissa , Herrera Genarino , Pimentel-Peralta Gumercindo , Landires Iván 
Published: October 2023

We aim to analyze Neisseria gonorrhoeae isolates in central Panama, characterize the associated gonococcal antimicrobial resistance (AMR) and conduct molecular epidemiology and genetic typing. We conducted a retrospective study based on N. gonorrhoeae hospital isolates collected between 2013 and 2018. AMR was determined using dilution agar and Etest®. Molecular typing was conducted using the Multilocus Sequence Typing (ST) scheme. The isolates analyzed (n = 30) showed resistance to penicillin (38%), tetracycline (40%), and ciprofloxacin (30%), and sensitivity to extended-spectrum cephalosporins and azithromycin. We identified 11 STs, the most frequent of which was ST1901 among the strains with decreased sensitivity and resistance to three types of antibiotics. We identified eight variations for the penA gene, all non-mosaic, with type II LVG as the most frequent (50%). To the best of our knowledge, we conducted the first Central American genomic study that analyzes a collection of gonococcal isolates, which represents a benchmark for future epidemiological and molecular genetic studies. The high prevalence of ciprofloxacin, tetracycline, and penicillin resistance, in addition to the identification of the worldwide spread of multidrug-resistant clone ST1901, should prompt the continuous and reinforced surveillance of AMR, including the molecular epidemiology of N. gonorrhoeae in Panama.
Steroidal Alkaloids from the Roots of Veratrum mengtzeanum Loes. with Their Anti-Inflammatory Activities
Authors: Yuan Wenjuan , Ma Jinrong , Liu Xinlan , Zi Chengting , Xi Yongkai , Shen Xiaojing , Li Guodong , Sheng Jun , Wang Xuanjun 
Published: October 2023

The phytochemical investigation of Veratrum mengtzeanum Loes. roots resulted in the isolation and characterization of two novel, namely Mengtzeanines A (1), Mengtzeanines B (2), and eight known steroidal alkaloids (3–10). Their structural properties were assessed though extensive spectroscopic techniques. All constituents 1–10 were analyzed for suppression of NO formation in LPS-induced RAW264.7 macrophages. Among them, constituent 6 (Verazine) showed inhibition against LPS-induced NO production (IC50 = 20.41 μM). Additionally, compound 6 could inhibit the secretion of IL1β, IL6, and TNFα, and downregulate the productions of iNOS and COX2 in LPS-induced RAW264.7 macrophages. Further experiments revealed that 6 exhibited a potent anti-inflammatory level in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB, and triggering of Keap1/Nrf2/HO-1 axis, implying that compound 6 may be a promising candidate for treating inflammatory disorders.
Construction of BiFeO3/SrTiO3 p–n heterojunction for enhanced photoelectrochemical performance
Authors: Nan Feng , Qiu Xin-Zhi , Lin Yi , Li Hao , Li Chun-Bo , Zhang Chun-Li , Zhou Lei 
Published: October 2023

Abstract
In this study, we design a hybrid BiFeO3/SrTiO3 p–n heterojunction to enhance the photoelectrochemical (PEC) performance of the sample. The experimental results reveal that the heterojunction can significantly improve the PEC performance. The optimized BiFeO3/SrTiO3 heterojunction sample shows a photocurrent density of −20.8 μA·cm−2, which is 2.9 times higher than that of the pristine BiFeO3 (−7.2 μA·cm−2). The improvement in PEC performance is attributed to the p–n heterojunction formed between BiFeO3 and SrTiO3, leading to the efficient separation and transport of carriers. Our work provides a promising route to develop the high-performance PEC system.
Fear-Neuro-Inspired Reinforcement Learning for Safe Autonomous Driving
Authors: He Xiangkun , Wu Jingda , Huang Zhiyu , Hu Zhongxu , Wang Jun , Sangiovanni-Vincentelli Alberto , Lv Chen 
Published: October 2023

<p>Ensuring safety and achieving human-level driving performance remain challenges for autonomous vehicles, especially in safety-critical situations. As a key component of artificial intelligence, reinforcement learning is promising and has shown great potential in many complex tasks; however, its lack of safety guarantees limits its real-world applicability. Hence, further advancing reinforcement learning, especially from the safety perspective, is of great importance for autonomous driving. As revealed by cognitive neuroscientists, the amygdala of the brain can elicit defensive responses against threats or hazards, which is crucial for survival in and adaptation to risky environments. Drawing inspiration from this scientific discovery, we present a fear-neuro-inspired reinforcement learning framework to realize safe autonomous driving through modeling the amygdala functionality. This new technique facilitates an agent to learn defensive behaviors and achieve safe decision making with fewer safety violations. Through experimental tests, we show that the proposed approach enables the autonomous driving agent to attain state-of-the-art performance compared to the baseline agents and perform comparably to 30 certified human drivers, across various safety-critical scenarios. The results demonstrate the feasibility and effectiveness of our framework while also shedding light on the crucial role of simulating the amygdala function in the application of reinforcement learning to safety-critical autonomous driving domains.</p>
Streamlined and Resource-Efficient Predictive Uncertainty Estimation of Deep Ensemble Predictions via Regression
Authors: Masakuna Jordan F. 
Published: October 2023

<p>This paper highlights the contribution of utilizing ensemble deep learning with auto-encoders (AEs) for out-of-distribution data detection. The key innovation is treating ensemble UQ as a regression problem, mapping uncertainty distribution to a single model, reducing computational demands. This approach aligns well with the ensemble of AEs' uncertainty distribution, making it valuable for resource-constrained systems and rapid decision-making in computational intelligence.</p>
Proton Craniospinal Irradiation with Immunotherapy in Two Patients with Leptomeningeal Disease from Melanoma
Authors: Sener Ugur , Webb Mason , Breen William G. , Neth Bryan J. , Laack Nadia N. , Routman David , Brown Paul D. , Mahajan Anita , Frechette Kelsey , Dudek Arkadiusz Z. , Markovic Svetomir N. , Block Matthew S. , McWilliams Robert R. , Dimou Anastasios , Kottschade Lisa A. , Montane Heather N. , Kizilbash Sani H. , Campian Jian L. 
Published: October 2023

ABSTRACT

Introduction
Proton craniospinal irradiation (pCSI) is a treatment option for leptomeningeal disease (LMD), which permits whole neuroaxis treatment while minimizing toxicity. Despite this, patients inevitably experience progression. Adding systemic therapy to pCSI may improve outcomes.

Methods
In this single-institution retrospective case series, we present the feasibility of treatment with pCSI (30Gy, 10 fractions) and an immune checkpoint inhibitor (ICI) in two sequential patients with LMD from melanoma.

Results
The first patient developed LMD related to BRAF V600E-mutant melanoma after prior ICI and BRAF-targeted therapy. After pCSI with concurrent nivolumab, the addition of relatlimab, and BRAF-targeted therapy, he remained alive 7 months after LMD diagnosis despite central nervous system progression. The second patient developed LMD related to BRAF-wildtype melanoma after up-front ICI. He received pCSI with concurrent ipilimumab and nivolumab, then nivolumab maintenance. Though therapy was held for ICI hepatitis, the patient remained progression-free 5 months after LMD diagnosis.

Conclusion
Adding an ICI to pCSI is feasible for patients with LMD and demonstrates a tolerable toxicity profile. While prospective evaluation is ultimately warranted, pCSI with ICI may confer survival benefits, even after prior ICI.