bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Safety of Contraction of Subcutaneous Tissue Following Liposuction Procedures
Authors: Shridharani Sachin M , Ruff Paul G , Doolabh Vaishali B , Zimmerman Edward M 
Published: December 2023

Abstract

This paper examines the practice of using a helium plasma radiofrequency (RF) device for contracting subcutaneous soft tissue following liposuction in all body areas. A review of the data from 6 industry-sponsor-initiated retrospective studies was performed, wherein 483 real-world patients underwent liposuction followed by contraction of the subcutaneous soft tissue with the helium plasma RF system. These data were evaluated to determine if any new or increased risks were introduced compared to the risks of liposuction alone. The totality of the real-world data demonstrates there are no new or increased risks for helium plasma RF procedures following liposuction compared to liposuction alone. These data support the safety of helium plasma RF for subcutaneous soft-tissue contraction following liposuction. There are currently no alternative therapies specifically cleared by the FDA that can claim use following liposuction for the purpose of contracting the subcutaneous soft tissue.

Level of Evidence: 3
PaintorPipe: a pipeline for genetic variant fine-mapping using functional annotations
Authors: Gerber Zoé , Fisun Michel , Aschard Hugues , Djebali Sarah 
Published: December 2023

Abstract

Motivation
Genome-wide association studies (GWAS) have identified thousands of genetic variants associated with common diseases. These results include a mix of causal and non-causal variants related through strong linkage disequilibrium (LD, i.e. highly correlated). Fine-mapping methods have been developed to decipher the causal from non-causal variants using GWAS results and LD information, assigning to each variant a probability of being causal. In this field, the PAINTOR program has become a standard, one of its advantages being its ability to take into account functional annotations. This approach requires many pre- and post-processing steps. Here, we developed a Nextflow pipeline called PaintorPipe that wraps all these steps and the fine-mapping itself together. PaintorPipe uses three independent sources of information: GWAS summary statistics, LD information and functional annotations, to rank the variants according to their susceptibility to be involved in the disease development. The PAINTOR framework is used to calculate the posterior probability of each variant (single nucleotide polymorphism) to be causal (a.k.a. Bayesian fine-mapping). The resulting credible sets of variants are annotated with their biological functions and visualized using CANVIS. This pipeline requires minimal input from users (a GWAS summary statistics file and a set of functional annotation files) and is designed to be modular and customizable, allowing for an easy integration of diverse functional annotations.

Availability and implementation
PaintorPipe is implemented in the Nextflow pipeline specific language, can be run locally or on a slurm cluster and handles containerization using Singularity. PaintorPipe is freely available on GitHub (https://github.com/sdjebali/PaintorPipe).
Effects of dopaminergic treatment on inhibitory control differ across Hoehn and Yahr stages of Parkinson’s disease
Authors: Mirabella Giovanni , Pilotto Andrea , Rizzardi Andrea , Montalti Martina , Olivola Enrica , Zatti Cinzia , Di Caprio Veronica , Ferrari Elisabetta , Modugno Nicola , Padovani Alessandro 
Published: December 2023

Abstract
Motor inhibitory control, a core component of cognitive control, is impaired in Parkinson’s disease, dramatically impacting patients’ abilities to implement goal-oriented adaptive strategies. A progressive loss of the midbrain’s dopamine neurons characterizes Parkinson’s disease and causes motor features responsive to dopaminergic treatments. Although such treatments restore motor symptoms, their impact on response inhibition is controversial. Most studies failed to show any effect of dopaminergic medicaments, although three studies found that these drugs selectively improved inhibitory control in early-stage patients. Importantly, all previous studies assessed only one domain of motor inhibition, i.e. reactive inhibition (the ability to react to a stop signal). The other domain, i.e. proactive inhibition (the ability to modulate reactive inhibition pre-emptively according to the current context), was utterly neglected. To re-examine this issue, we recruited cognitively unimpaired Parkinson’s patients under dopaminergic treatment in the early (Hoehn and Yahr, 1–1.5, n = 20), intermediate (Hoehn and Yahr 2, n = 20), and moderate/advanced (Hoehn and Yahr, 2.5–3, n = 20) stages of the disease. Using a cross-sectional study design, we compared their performance on a simple reaction-time task and a stop-signal task randomly performed twice on dopaminergic medication (ON) and after medication withdrawal (OFF). Normative data were collected on 30 healthy controls. Results suggest that medication effects are stage-dependent. In Hoehn and Yahr 1–1.5 patients, drugs selectively impair reactive inhibition, leaving proactive inhibition unaffected. In the ON state, Hoehn and Yahr two patients experienced impaired proactive inhibition, whereas reactive inhibition is no longer affected, as it deteriorates even during the OFF state. By contrast, Hoehn and Yahr 2.5–3 patients exhibited less efficient reactive and proactive inhibition in the OFF state, and medication slightly improved proactive inhibition. This evidence aligns with the dopamine overdose hypothesis, indicating that drug administration may overdose intact dopamine circuitry in the earliest stages, impairing associated cognitive functions. In later stages, the progressive degeneration of dopaminergic neurons prevents the overdose and can exert some beneficial effects. Thus, our findings suggest that inhibitory control assessment might help tailor pharmacological therapy across the disease stage to enhance Parkinson’s disease patients’ quality of life by minimizing the hampering of inhibitory control and maximizing the reduction of motor symptoms.
Association of endomyocardial fibrosis and minor myocarditis sequelae with intracardiac thrombus and Ebstein like valvulopathy in a patient with Behçet disease: a case report
Authors: Ceruti Hélène , van de Borne Philippe , Mirica Daniela-Corina 
Published: December 2023

Abstract

Background
Cardiac complications occur in 1–6% of cases of Behçet disease (BD) with intracardiac thrombus being the most frequent complication. Endomyocardial fibrosis, less common and occasionally associated with intracardiac thrombus, is reported in &lt;20 case reports of BD, among which, three cases are described to mimic Ebstein disease based on echocardiography. We present the first case in the literature of a 34-year-old man with BD diagnosed with multiple cardiovascular complications, highlighting the challenging diagnosis and treatment of this pathology, especially regarding anticoagulation therapy.

Case summary
A 34-year-old man, diagnosed with BD, presented to the Emergency Room with haemoptysis. Computed tomography study of the thorax diagnosed pulmonary arterial aneurysm with multiple arterial thrombi, associated with multiple intracardiac thrombi in the right ventricle and atrium. The echocardiography confirmed the presence of voluminous thrombi in the right ventricle and atrium and showed hypertrabeculation of the right ventricle and a high insertion of the posterior leaflet of the tricuspid valve inducing a moderate tricuspid insufficiency compatible with an Ebstein disease. The cardiac MRI later revealed right ventricular fibrosis consistent with endomyocardial fibrosis and sequelae of myocarditis, also described as BD rare cardiac manifestations. The patient had a favourable outcome under anticoagulant treatment and immunosuppressive drugs.

Discussion
The association of multiple cardiovascular complications can occur in a single patient with BD. The endomyocardial fibrosis in the right heart chambers acting as a substrate for thrombus formation and subsequent pulmonary embolism; fibrosis extending to the tricuspid valve inducing an Ebstein-like morphology.
Machine learning–based gait analysis to predict clinical frailty scale in elderly patients with heart failure
Authors: Mizuguchi Yoshifumi , Nakao Motoki , Nagai Toshiyuki , Takahashi Yuki , Abe Takahiro , Kakinoki Shigeo , Imagawa Shogo , Matsutani Kenichi , Saito Takahiko , Takahashi Masashige , Kato Yoshiya , Komoriyama Hirokazu , Hagiwara Hikaru , Hirata Kenji , Ogawa Takahiro , Shimizu Takuto , Otsu Manabu , Chiyo Kunihiro , Anzai Toshihisa 
Published: December 2023

Abstract

Aims
Although frailty assessment is recommended for guiding treatment strategies and outcome prediction in elderly patients with heart failure (HF), most frailty scales are subjective, and the scores vary among raters. We sought to develop a machine learning–based automatic rating method/system/model of the clinical frailty scale (CFS) for patients with HF.

Methods and results
We prospectively examined 417 elderly (≥75 years) with symptomatic chronic HF patients from 7 centres between January 2019 and October 2023. The patients were divided into derivation (n = 194) and validation (n = 223) cohorts. We obtained body-tracking motion data using a deep learning–based pose estimation library, on a smartphone camera. Predicted CFS was calculated from 128 key features, including gait parameters, using the light gradient boosting machine (LightGBM) model. To evaluate the performance of this model, we calculated Cohen’s weighted kappa (CWK) and intraclass correlation coefficient (ICC) between the predicted and actual CFSs. In the derivation and validation datasets, the LightGBM models showed excellent agreements between the actual and predicted CFSs [CWK 0.866, 95% confidence interval (CI) 0.807–0.911; ICC 0.866, 95% CI 0.827–0.898; CWK 0.812, 95% CI 0.752–0.868; ICC 0.813, 95% CI 0.761–0.854, respectively]. During a median follow-up period of 391 (inter-quartile range 273–617) days, the higher predicted CFS was independently associated with a higher risk of all-cause death (hazard ratio 1.60, 95% CI 1.02–2.50) after adjusting for significant prognostic covariates.

Conclusion
Machine learning–based algorithms of automatically CFS rating are feasible, and the predicted CFS is associated with the risk of all-cause death in elderly patients with HF.
Current national availability of advanced echocardiography imaging: real world data from an Italian Society of Echocardiography and Cardiovascular Imaging survey
Authors: Barbieri Andrea , Mantovani Francesca , Ciampi Quirino , Barchitta Agata , Faganello Giorgio , Miceli Sofia , Parato Vito Maurizio , Tota Antonio , Trocino Giuseppe , Antonini-Canterin Francesco , Carerj Scipione , Pepi Mauro 
Published: December 2023

AbstractAimsAdvanced echocardiographic imaging (AEI) techniques, such as three-dimensional (3D) and multi-chamber speckle-tracking deformation imaging (strain) analysis, have been shown to be more accurate in assessing heart chamber geometry and function when compared with conventional echocardiography providing additional prognostic value. However, incorporating AEI alongside standard examinations may be heterogeneous between echo laboratories (echo labs). Thus, our goal was to gain a better understanding of the many AEI modalities that are available and employed in Italy.Methods and resultsThe Italian Society of Echocardiography and Cardiovascular Imaging (SIECVI) conducted a national survey over a month (November 2022) to describe the use of AEI in Italy. Data were retrieved via an electronic survey based on a structured questionnaire uploaded on the SIECVI website. Data obtained from 173 echo labs were divided into 3 groups, according to the numbers of echocardiograms performed: &lt;250 exams (low-volume activity, 53 centres), between 251 and 550 exams (moderate-volume activity, 62 centres), and ≥550 exams (high-volume activity, 58 centres). Transthoracic echocardiography (TTE) 3D was in use in 75% of centres with a consistent difference between low (55%), medium (71%), and high activity volume (85%) (P = 0.002), while 3D transoesophageal echocardiography (TEE) was in use in 84% of centres, reaching the 95% in high activity volume echo labs (P = 0.006). In centres with available 3D TTE, it was used for the left ventricle (LV) analysis in 67%, for the right ventricle (RV) in 45%, and for the left atrium (LA) in 40%, showing greater use in high-volume centres compared with low- and medium-volume centres (all P &lt; 0.04). Strain analysis was utilized in most echo labs (80%), with a trend towards greater use in high-volume centres than low- and medium-volume centres (77%, 74%, and 90%, respectively; P = 0.08). In centres with available strain analysis, it was mainly employed for the LV (80%) and much less frequently for the RV and LA (49% and 48%, respectively).ConclusionIn Italy, the AEI modalities are more frequently available in centres with high-volume activity but employed only in a few applications, being more frequent in analysing the LV compared with the RV and LA. Therefore, the echocardiography community and SIECVI should promote uniformity and effective training across the Italian centres. Meanwhile, collaborations across centres with various resources and expertise should be encouraged to use the benefits of the AEI.
Mirroring the American Bankruptcy Code? IP Licences in the European Insolvency Harmonisation Project
Authors: Gabriel-Pizarro Guillem 
Published: December 2023

Abstract
With the growth of the licensing of Intellectual Property rights in the global economy, the insolvency treatment of licensing agreements has also taken on greater importance. This paper analyses the treatment of intellectual property licences under the European Commission’s new Directive Proposal 2022/0408/COD, the flagship law of the European insolvency harmonisation project. The paper focuses on the Proposal’s approach in cases where the debtor’s business, or part of it, is sold as a going concern in the pre-pack context. It contrasts the European response to the US Bankruptcy Code, which serves as a significant source of inspiration, and provides examples of how some EU Member States handle such cases.
In substance, the paper examines the Commission’s steps towards harmonising the treatment of IP licences in insolvency. Considering the insolvency context and the needs of the parties to the licence, it critically analyses not only the expediency of implementing specific regulations but also the effectiveness of the means employed. Finally, the paper provides some suggestions to amend the Proposal and highlights the necessity to revisit the broader debate over the treatment of IP licences in European insolvency legislation in general.
Impact of COVID-19 on incidence and outcomes of post-infarction mechanical complications in Europe
Authors: Ronco Daniele , Matteucci Matteo , Ravaux Justine Mafalda , Kowalewski Mariusz , Massimi Giulio , Torchio Federica , Trumello Cinzia , Naito Shiho , Bonaros Nikolaos , De Bonis Michele , Fina Dario , Kowalówka Adam , Deja Marek , Jiritano Federica , Serraino Giuseppe Filiberto , Kalisnik Jurij Matija , De Vincentiis Carlo , Ranucci Marco , Fischlein Theodor , Russo Claudio Francesco , Carrozzini Massimiliano , Boeken Udo , Kalampokas Nikolaos , Golino Michele , De Ponti Roberto , Pozzi Matteo , Obadia Jean-François , Thielmann Matthias , Scrofani Roberto , Blasi Stefania , Troise Giovanni , Antona Carlo , De Martino Andrea , Falcetta Giosuè , Actis Dato Guglielmo , Severgnini Paolo , Musazzi Andrea , Lorusso Roberto 
Published: December 2023

Abstract

OBJECTIVES
Post-acute myocardial infarction mechanical complications (post-AMI MCs) represent rare but life-threatening conditions, including free-wall rupture, ventricular septal rupture and papillary muscle rupture. During the coronavirus disease-19 (COVID-19) pandemic, an overwhelming pressure on healthcare systems led to delayed and potentially suboptimal treatments for time-dependent conditions. As AMI-related hospitalizations decreased, limited information is available whether higher rates of post-AMI MCs and related deaths occurred in this setting. This study was aimed to assess how COVID-19 in Europe has impacted the incidence, treatment and outcome of MCs.

METHODS
The CAUTION-COVID19 study is a multicentre retrospective study collecting 175 patients with post-AMI MCs in 18 centres from 6 European countries, aimed to compare the incidence of such events, related patients’ characteristics, and outcomes, between the first year of pandemic and the 2 previous years.

RESULTS
A non-significant increase in MCs was observed [odds ratio (OR) = 1.15, 95% confidence interval (CI) 0.85–1.57; P = 0.364], with stronger growth in ventricular septal rupture diagnoses (OR = 1.43, 95% CI 0.95–2.18; P = 0.090). No significant differences in treatment types and mortality were found between the 2 periods. In-hospital mortality was 50.9% and was higher for conservatively managed cases (90.9%) and lower for surgical patients (44.0%). Patients admitted during COVID-19 more frequently had late-presenting infarction (OR = 2.47, 95% CI 1.24–4.92; P = 0.010), more stable conditions (OR = 2.61, 95% CI 1.27–5.35; P = 0.009) and higher EuroSCORE II (OR = 1.04, 95% CI 1.01–1.06; P = 0.006).

CONCLUSIONS
A non-significant increase in MCs incidence occurred during the first year of COVID-19, characterized by a significantly higher rate of late-presenting infarction, stable conditions and EuroSCORE-II if compared to pre-pandemic data, without affecting treatment and mortality.
Ex vivo comparative immunogenicity assessment (EVCIA) to determine relative immunogenicity in chronic plaque psoriasis in participants receiving Humira® or undergoing repeated switches between Humira® and AVT02
Authors: Richter Kathleen , Haliduola Halimu N , Schockaert Jana , Mazy Aurélie , Reznichenko Nataliya , Guenzi Eric , Berti Fausto 
Published: December 2023

Summary
Immunogenicity against biologic medicines is ubiquitous, and it is traditionally measured by the final humoral response. However, the onset of a sustained immunogenic response begins at the cellular level with activation of T cells and maturation of naïve B cells into plasma cells. Ex vivo comparative immunogenicity assessment (EVCIA) of cellular immunogenicity in participants with moderate-to-severe chronic plaque psoriasis in the AVT02-GL-302 study, who received either reference product (RP) alone (non-switching arm) or switched between RP and AVT02 (switching arm) after 1:1 randomization at week 12. Peripheral blood mononuclear cells (PBMCs) were collected and cryopreserved from 28 participants at: baseline (before treatment) (week 1); pre-randomization (week 12); and week 16 and week 28 in both switching and non-switching arms. PBMCs were thawed and re-exposed to either medium alone (negative control), RP, AVT02, keyhole limpet hemocyanin (KLH) (positive control), RP+KLH, or AVT02+KLH. Samples from 10 participants (predetermined average cell viability of 75% across all timepoints) from each arm were analyzed for cytokine release after 24 hours and for Th-cell proliferation, 6 days post-seeding. Until week 28, cytokine release and Th-cell proliferation was similar at all time points in both switching and non-switching arms. Overall cellular immune response was elevated post-KLH re-exposure at all timepoints. The comparable ex vivo cellular immunogenicity between switching and non-switching arms complements the confirmation of interchangeability in the main study. Given the sensitivity of novel EVCIA, detecting cellular immunogenicity could be a potential outcome in predicting the immunogenicity of biologic medicines.
Africa's Great Moderation
Authors: Krantz Sebastian 
Published: December 2023

Abstract
Over the past 30 years, African economies have experienced remarkable improvements in macroeconomic conditions, characterised by higher and more stable real per-capita growth rates and lower and more stable inflation. This paper documents the persistent decline in macroeconomic volatility at the aggregate and sectoral levels and seeks to provide explanations. Sectoral analysis shows a particularly strong reduction of growth volatility in agriculture and, to a lesser extent, in services. Classical structural change only explains a small fraction of the moderation. Analysis of further factors yields that changes in structural characteristics such as institutions, trade intensity and diversification, natural resource dependence or conflict incidence do not explain the moderation. On the positive side, the paper provides evidence to suggest that changes in the external environment, improved macroeconomic policy frameworks and ‘softer’ structural improvements, such as the deepening of the domestic financial sector, were important in reducing macroeconomic volatility on the continent.
Sympatry and parapatry among rocky reef cichlids of Lake Victoria explained by female mating preferences
Authors: Svensson Ola , Woodhouse Katie , Smith Alan , Seehausen Ole , Turner George F 
Published: December 2023

Abstract
Work on the Lake Victoria cichlids Pundamilia nyererei (red dorsum males, deeper water), Pundamilia pundamilia (blue males, shallower water) and related species pairs has provided insights into processes of speciation. Here, we investigate the female mating behaviour of 5 Pundamilia species and 4 of their F1 hybrids through mate choice trials and paternity testing. Complete assortative mating was observed among all sympatric species. Parapatric species with similar depth habitat distributions interbred whereas other parapatric and allopatric species showed complete assortative mating. F1 hybrids mated exclusively with species accepted by females of the parental species. The existence of complete assortative mating among some currently allopatric species suggests that pre-existing mating barriers could be sufficient to explain current patterns of co-existence, although, of course, many other factors may be involved. Regardless of the mechanism, mating preferences may influence species distribution in potentially hybridizing taxa, such as in the adaptive radiation of cichlid fish. We suggest that this at least partly explains why some species fail to establish breeding populations in locations where they are occasionally recorded. Our results support the notion that the mating preferences of potentially cross-breeding species ought to be included in coexistence theory.