bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber
Authors: Zuponcic Jessica , Cunha Fernanda , Springer Grant , Ximenes Eduardo , Ladisch Michael R. 
Published: February 2024

AbstractPathogenic bacteria which enter a viable but non‐culturable (VBNC) state impede efforts to reach detectable concentrations required for PCR methods. This motivated a strategy for tangential flow filtration to concentrate bacteria in aqueous samples while maintaining the bacteria in a viable state, maximizing their recovery and achieving high fluxes through a single hollow fiber membrane. Filtrations were carried out for green fluorescent protein (GFP) E. coli at high shear rates (up to 27,000 sec−1) through 0.2 μm cut‐off polyethersulfone (PES) microfilter membranes or 50 kDa polysulfone (PS) ultrafilter membranes. High shear minimized bacterial attachment on membrane surfaces, which would otherwise occur due to forced convection of the particles to the membrane surface at high flux conditions. Single fiber filter modules were constructed to facilitate concentration of Escherichia coli at fluxes ranging from 55 to 4500 L m−2 h−1. The effect of high shear rates on bacterial viability was found to be minimal with bacterial losses during filtration caused principally by their accumulation on the membrane surface. Recoveries of 90% were achievable at high shear rates when the average flux was ≤300 L m−2 h−1. This corresponded to a 3‐h filtration time for a 225 mL sample through a single hollow fiber. Detectable bacteria concentrations of 1800 colony‐forming unit (CFU)/mL were achieved for starting concentrations of 140 CFU/mL.
New Iridoids and Acyclic Monoterpenoids from the Roots and Rhizomes of Valeriana officinalis var. latifolia
Authors: Liu Jia‐Jin , Wang Li‐Zhong , Duan Yuan‐Ting , Liu Dan , Li Hong‐Mei , Li Rong‐Tao 
Published: February 2024

AbstractFive new iridoids, valeralides A–E (1–5), two new acyclic monoterpenoids, valeralides F (6) and G (7), together with two known iridoids (8 and 9), were isolated from the roots and rhizomes of Valeriana officinalis var. latifolia. Their structures were elucidated based on 1D and 2D NMR, as well as HR‐ESI‐MS spectroscopic data. The absolute configuration of compounds 1–4 were elucidated based on electronic circular dichroism (ECD) calculation. In addition, all the isolates were evaluated for their inhibition on nitric oxide production, cytotoxicity and anti‐influenza A virus activity.
The Biological Activities of Polyether Ionophore Antibiotic Routiennocin is Independent of Absolute Stereochemistry
Authors: Borcher Møller Søren Lau , Rasmussen Michelle H. , Li Jun , Svenningsen Esben B. , Wørmer Gustav J. , Tørring Thomas , Poulsen Thomas B. 
Published: February 2024

AbstractCarboxylic polyether ionophores (CPIs) are among the most prevalent agricultural antibiotics (notably in the US) and these compounds have been in use for decades. The potential to reposition CPIs beyond veterinary use, e. g. through chemical modifications to enhance their selectivity window, is an exciting challenge and opportunity, considering their general resilience towards resistance development. Given the very large societal impact of these somewhat controversial compounds, it is surprising that many aspects of their mechanisms and activities in cells remain unclear. Here, we report comparative biological activities of the CPI routiennocin and two stereoisomers, including its enantiomer. We used an efficient convergent synthesis strategy to access the compounds and conducted a broad survey of antibacterial activities against planktonic cells and biofilms as well as the compounds’ effects on mammalian cells, the latter assessed both via standard cell viability assays and broad morphological profiling. Interestingly, similar bioactivity of the enantiomeric pair was observed across all assays, strongly suggesting that chiral interactions do not play a decisive role in the mode of action. Overall, our findings are consistent with a mechanistic model involving highly dynamic behaviour of CPIs in biological membranes.
Portal vein thrombosis in a patient with severe hemophilia B: A challenging balanced management
Authors: Benitez‐Hidalgo Olga , Suito Alcántara Milagros , Martinez Garcia Maria Fernanda , Campoy Desiree , Olivera Pavel , Gironella Mesa Mercedes , Juarez‐Gimenez Juan Carlos 
Published: February 2024

Key Clinical MessageThe increased life expectancy in patients with hemophilia (PwH) over the last years has raised the incidence of comorbidities, including thromboembolic events. Thromboembolic events are rare in PwH and most of them occur in the presence of exogenous risk factors. There is still scarce scientific evidence on the optimal antithrombotic treatment and management approach in this population.AbstractIn the hemophilic population thromboembolic events are rare. Most of them are often multifactorial and occur in the presence of both exogenous (orthopedic surgery, intensive replacement therapy, use of central venous catheters…) and endogenous (cardiovascular diseases) risk factors. We describe the case of a 43‐year‐old patient with severe hemophilia B (sHB) receiving prophylaxis with eftrenonacog alfa (rFIXFc) and antithrombotic treatment due to portal vein thrombosis. The patient was treated with extended half‐ life factor IX (EHL‐FIX) prophylaxis maintaining higher trough levels to avoid new bleeding episodes associated to the underlying disease and the use of antithrombotic therapy with low molecular weight heparin. EHL‐FIX concentrates allow prolonged intervals between intravenous infusions and higher hemostatic protection thanks to increased factor trough levels. This current case report provides clinical evidence in antithrombotic management in a patient with severe hemophilia B.
A five‐day‐old child with lipid hemihypertrophy: A case report
Authors: Mirnia Kayvan , Saeedi Maryam , Sangsari Razieh , Kazemzadeh Kimia 
Published: February 2024

Key Clinical MessageLipid hemihypertrophy should be considered in the differential diagnosis of neonatal asymmetry. Early recognition and further evaluation for associated disorders are important for appropriate management and surveillance of potential complications.AbstractWe present the case of a 5‐day‐old female neonate who presented with a visibly enlarged right thigh, right labia majora, and below the right mandible. This case report highlights the importance of early identification, comprehensive evaluation, and multidisciplinary management in neonates with lipid hemihypertrophy to optimize their long‐term outcomes and quality of life.
A couple of the first cousins born with hypotonia and maternal polyhydramnios
Authors: Ahmadpour‐kacho Mousa , Pasha Yadollah Zahed , Pournajaf Samira 
Published: February 2024

Key Clinical MessageCongenital myotonic dystrophy should be considered in hypotonic infants with polyhydramniotic mothers with a positive history of myotonia.AbstractCongenital myotonic dystrophy (CDM) is a predominantly maternally inherited disease and results from increased numbers of cytosine, thymine, and guanine (CTG) repeats in the unstable DNA regions and presents as hypotonia in the neonatal period and myotonia in adulthood. This report aims to present two cases of CDM. A first‐cousin couple was born and hospitalized due to hypotonia at birth and a maternal history of polyhydramnios during this pregnancy. The first‐born baby girl was admitted to the NICU with tachypnea and hypotonia, clubfoot, and frog‐like posture. The pregnancy was complicated by polyhydramnios. Interestingly, her first cousin was born the next day with a similar picture and history. Myotonia was detected in their mothers. The concurrent presence of hypotonia and polyhydramnios as well as maternal myotonia in a first cousin should be considered CDM until proven otherwise and this was confirmed by the EMG‐ NCV test.
JAK3/STAT5 signaling‐triggered upregulation of PIK3CD contributes to gastric carcinoma development
Authors: Hu Qingqing , Dou Ning , Wu Qiong , Gao Yong , Li Yandong , Chen Jingde 
Published: February 2024

AbstractGastric cancer (GC) is one of the most common solid cancers with high incidence and mortality worldwide. Chronic gastritis and consequent inflammatory microenvironment is known as a major cause leading to gastric carcinogenesis. Here we report that PIK3CD that encodes p110δ, a catalytic subunit of the class IA PI3Ks, is overexpressed and tumorigenic in GC and associated with tumor inflammatory microenvironment. By investigating the data from TCGA database and our immunohistochemical staining and quantitative real‐time PCR results from clinical samples, we found PIK3CD exhibits higher expression level in GC tissues compared with adjacent non‐tumorous stomach tissues. Genetic silencing of PIK3CD in GC cells retards proliferation and migration in vitro and tumorigenicity and metastasis in vivo. In contrast, enhanced expression of PIK3CD promotes these phenotypes in vitro. Furthermore, pharmacological inhibition of PIK3CD could reduce GC cell viability and colony formation capacities. More importantly, we reveal a relevant mechanism that PIK3CD, but not PIK3CA and PIK3CB, is transcriptionally regulated by the pro‐inflammatory IL2/JAK3/STAT5 axis and tumor‐infiltrating immune cells such as lymphocytes. These observations may setup a new crosstalk between tumor inflammatory microenvironment, IL2/JAK3/STAT5 signaling and PI3K/AKT signaling. Targeting PIK3CD may be a promising therapy strategy for GC.
The influence of the Cr2O3–Mn2O3 colorants system on the spectral characteristics of R2O–PbO–SiO2 glasses
Authors: Petrov D. V. , Bragina L. L. 
Published: February 2024

AbstractThe focus of this paper centers on an exploration of optical glasses with high transmittance within the infrared spectrum at wavelength of 1 060 nm, while concurrently demonstrating full absorption within the ultraviolet and visible spectral ranges. The paper analyzes the present state of glasses demonstrating necessary transmittance at IR range, considering their inherent strengths and limitations. In this context, an R2O–PbO–SiO2 glass system with Cr2O3–Mn2O3 colorants system emerges as the subject of this research. Research was conducted to comprehensively assess the impact of this colorant system on the glass spectral characteristics, while considering the potentially detrimental influence of FeO.The principles underlying the industrial production of these glasses are systematically elucidated and presented. It was discovered that using gas heating during the cooling phase of glass melting resulted in the disruption of the spectral homogeneity of the glass, ultimately leading to defective product outcomes. Implementing the methodology of gasless temperature reduction (inertial cooling process) before glass pouring has created a favorable environment for producing high‐quality items. Glass samples from the laboratory tests were obtained, and the researched glass was integrated into serial production.
Assessment and Upgrading of Preparation Protocols for Emulsified Crude Oils Mimicking Real Produced Water Characteristics
Authors: Idrees Hasan , Alhanini Heba , Panglisch Stefan , ElSherbiny Ibrahim M. A. 
Published: February 2024

AbstractProduced water (PW) presents a significant challenge as a complex wastewater stream, necessitating thorough treatment prior to disposal or further reuse. This study is dedicated to the assessment, reproduction and upgrading of literature‐based preparation protocols for synthetic PW at the tertiary treatment stage; more specifically those methods utilizing high‐pressure homogenizers and ultrasonication. The objective was to produce synthetic model water that can be employed as a standardized controllable medium for optimization experiments of PW treatment techniques. This synthetic medium should facilitate the investigation of specific challenges associated with PW tertiary treatment, independent of the different and often unpredictable composition of real PW. The refined methods were modified for the application in lab‐scale research investigating the use of membrane filtration as tertiary treatment step.
Synthesis and in vitro Metabolic Stability of Sterically Shielded Antimycobacterial Phenylalanine Amides
Authors: Lang Markus , Ganapathy Uday S. , Mann Lea , Seidel Rüdiger W. , Goddard Richard , Erdmann Frank , Dick Thomas , Richter Adrian 
Published: February 2024

AbstractNα‐aroyl‐N‐aryl‐phenylalanine amides (AAPs) are RNA polymerase inhibitors with activity against Mycobacterium tuberculosis and non‐tuberculous mycobacteria. We observed that AAPs rapidly degrade in microsomal suspensions, suggesting that avoiding hepatic metabolism is critical for their effectiveness in vivo. As both amide bonds are potential metabolic weak points of the molecule, we synthesized 16 novel AAP analogs in which the amide bonds are shielded by methyl or fluoro substituents in close proximity. Some derivatives show improved microsomal stability, while being plasma‐stable and non‐cytotoxic. In parallel with the metabolic stability studies, the antimycobacterial activity of the AAPs against Mycobacterium tuberculosis, Mycobacterium abscessus, Mycobacterium avium and Mycobacterium intracellulare was determined. The stability data are discussed in relation to the antimycobacterial activity of the panel of compounds and reveal that the concept of steric shielding of the anilide groups by a fluoro substituent has the potential to improve the stability and bioavailability of AAPs.
Self‐etching assembly of designed NiFeMOF nanosheet arrays as high‐efficient oxygen evolution electrocatalyst for water splitting
Authors: Zhang Shifan , Liao Miao , Huang Zhiyang , Gao Mingcheng , Liu Xinqiang , Yin Haoran , Isimjan Tayirjan Taylor , Cai Dandan , Yang Xiulin 
Published: February 2024

Abstract2D metal‐organic frameworks (MOFs) have emerged as potential candidates for electrocatalytic oxygen evolution reactions (OER) due to their inherent properties like abundant coordination unsaturated active sites and efficient charge transfer. Herein, a versatile and massively synthesizable self‐etching assembly strategy wherein nickel−iron foam (NFF) acts as a substrate and a metal ion source. Specifically, by etching the nickel−iron foam (NFF) surface using ligands and solvents, Ni/Fe metal ions are activated and subsequently reacted under hydrothermal conditions, resulting in the formation of self‐supporting nanosheet arrays, eliminating the need for external metal salts. The obtained 33 % NiFeMOF/NFF exhibits remarkable OER performance with ultra‐low overpotentials of 188/231 mV at 10/100 mA cm−2, respectively, outperforming most recently reported catalysts. Besides, the built 33 % NiFeMOF/NFF(+)||Pt/C(−) electrolyzer presents low cell voltages of 1.55/1.83 V at 10/100 mA cm−2, superior to the benchmark RuO2(+)||Pt/C(−), implying good industrialization prospects. The excellent catalytic activity stems from the modulation of the electronic spin state of the Ni active site by the introduction of Fe, which facilitates the adsorption process of oxygen‐containing intermediates and thus enhances the OER activity. This innovative approach offers a promising pathway for commercial‐scale sustainable energy solutions.
The challenge of standardizing CAR‐T cell monitoring: A comparison of two flow‐cytometry methods and correlation with qPCR technique
Authors: Valdivieso‐Shephard Juan Luis , Matas‐Pérez Elisabet , García‐Bujalance Silvia , Mirones‐Aguilar Isabel , González‐Martínez Berta , Pérez‐Martínez Antonio , López‐Granados Eduardo , Martínez‐Feito Ana , Sánchez‐Zapardiel Elena 
Published: February 2024

AbstractChimeric antigen receptor (CAR) T‐cell therapy is a breakthrough in hematologic malignancies, such as acute B lymphoblastic leukemia (B‐ALL). Monitoring this treatment is recommended, although standardized protocols have not been developed yet. This work compares two flow cytometry monitoring strategies and correlates this technique with qPCR method. CAR‐T cells were detected by two different flow‐cytometry protocols (A and B) in nine blood samples from one healthy donor and five B‐ALL patients treated with Tisagenlecleucel (Kymriah®, USA). HIV‐1 viral load allowed CAR detection by qPCR, using samples from seven healthy donors and nine B‐ALL patients. CAR detection by protocol A and B did not yield statistically significant differences (1.9% vs. 11.8% CD3 + CAR+, p = 0.07). However, protocol B showed a better discrimination of the CD3 + CAR+ population. A strong correlation was observed between protocol B and qPCR (r = 0.7, p < 0.0001). CD3 + CAR+ cells were detected by flow cytometry only when HIV‐1 viral load was above 104 copies/mL. In conclusion, protocol B was the most specific flow‐cytometry procedure for the identification of CAR‐T cells and showed a high correlation with qPCR. Further efforts are needed to achieve a standardized monitoring approach.
Dynamic, multi‐scale analyses indicate site‐ and landscape‐level forest cover drive Yellow‐billed and Black‐billed Cuckoo interannual turnover
Authors: Johnson Claire A. , Benson Thomas J. 
Published: February 2024

AbstractStudies of habitat use in breeding birds often assume species have relatively stable breeding distributions. Some species, however, display considerable year‐to‐year variability, complicating efforts to determine suitable or preferred habitats. After returning to their breeding range, Black‐billed Cuckoos (Coccyzus erythropthalmus) and Yellow‐billed Cuckoos (C. americanus) are thought to range widely before nesting, resulting in high rates of interannual breeding‐site turnover, potentially contributing to conflicting habitat associations found in past studies. However, difficulty detecting these rare and declining species could lead to overinflated estimates of interannual turnover. Using broadcast surveys to increase detection probability, we collected detection/non‐detection data in 2019 and 2020 at 41 publicly owned sites in Illinois and performed a dynamic, multi‐scale occupancy analysis for each species to separate detection probability from potential interannual turnover and determine landscape and small‐scale variables driving habitat use and occupancy dynamics. We found strong support for interannual turnover for both species based on poor performance of non‐dynamic models and variation in estimated annual occupancy (20% and 21% increase between years for Black‐billed and Yellow‐billed Cuckoos, respectively). Black‐billed Cuckoos persisted at sites with less forest in the surrounding landscape and used areas with denser understory vegetation. Yellow‐billed Cuckoos colonized sites with greater canopy cover, avoided developed landscapes, and used areas with a shorter subcanopy layer. The dynamic nature of habitat use in these two cuckoo species suggests the importance of coordinating management and conservation across a broader spatial scale. Managing for larger patches of dense shrubs in less forested landscapes would benefit Black‐billed Cuckoos while Yellow‐billed cuckoos would benefit from management creating forested areas with open understories in less‐developed landscapes.
1,5,7‐Triazabicyclo[4.4.0]dec‐5‐ene (TBD): An Organocatalyst for Rapid Access to 3‐Hydroxyisoindolin‐1‐ones
Authors: Lan Chunling Blue , Auclair Karine 
Published: February 2024

AbstractThe readily available organocatalyst 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene (TBD) was used for the rapid synthesis of 3‐hydroxyisoindolin‐1‐ones from 3‐alkylidenephthalides. The transformation occurs at room temperature and requires less solvent than traditional methods, providing a more sustainable synthetic option to access 3‐hydroxyisoindolin‐1‐ones with broad scope. Elaboration of the products to diverse scaffolds as well as synthesis of biologically active compounds are demonstrated.
The integrated farming system is an environmentally friendly and cost‐effective approach to the sustainability of agri‐food systems in the modern era of the changing climate: A comprehensive review
Authors: Bhagat Rakshit , Walia Sohan Singh , Sharma Kartik , Singh Rajbir , Singh Gurshaminder , Hossain Akbar 
Published: February 2024

AbstractThe integrated farming system (IFS) is a holistic farming approach specially designed for small/marginal farmers to enhance the system productivity, profitability, and employment generation of their farm, ultimately ensuring their food and nutrition security for their livelihood. The mechanism of IFS offers various ecosystem services making farms environment‐friendly, sustainable, and climate‐resilient. IFS is characterized by the allocation of different agricultural components systematically in a single farm performing synergistically among themselves making the farm more productive, healthy, biodiversity enriched, and eco‐friendly than simplified farms. Therefore, this review paper represents an extensive analysis of existing literature on IFS exploring its concept, components, and need, and emphasizing its potential for higher system productivity, profitability, and employment generation in a sustainable manner. Moreover, it develops a conceptualization of the relationship among different agricultural components in a single farm exploring its role in ecosystem services (reducing greenhouse gases, carbon sequestration, nutrient recycling, improving soil health, and biodiversity conservation).
Immunomodulatory activity of heat‐killed Lacticaseibacillus paracaseiMCC1849 based on the activation of plasmacytoid dendritic cells in the peripheral blood of healthy adults
Authors: Li Yiran , Aoki Takahiro , Iwabuchi Sadahiro , Arai Satoshi , Iwabuchi Noriyuki , Motobayashi Hideki , Tanaka Miyuki , Hashimoto Shinichi 
Published: February 2024

AbstractProbiotics are widely used in food for their health benefits to the host. Inactivated probiotics also reportedly improve the intestinal environment and immune regulation. Our previous studies showed that heat‐killed Lacticaseibacillus paracasei MCC1849 (hk‐MCC1849) effectively induced IL‐12 production in mouse spleen cells and significantly reduced cold symptoms in clinical trial subjects. To further elucidate the mechanism of host immune regulation by hk‐MCC1849, human peripheral blood mononuclear cells (PBMCs) were cocultured with hk‐MCC1849. The Toll‐like receptor 9 ligands CpG‐ODN 2216 and hk‐MCC1849 and the heat‐killed Lacticaseibacillus rhamnosus ATCC53103 were used as positive and negative controls, respectively. The results showed that, compared with the control, hk‐MCC1849 significantly increased the expression of the plasmacytoid dendritic cell (pDC) marker CD86 (p < .0001) and the pDC marker HLA‐DR (p < .001) in PBMCs. The expression levels of the IL‐12p40, IFNα, IFNα1, IFNγ, and ISG15 genes were significantly increased after coculture with hk‐MCC1849 (p < .05, p < .05, p < .05, p < .05, and p < .05, respectively, vs. control). Furthermore, to confirm whether hk‐MCC1849 directly interacted with pDCs, DCs were enriched with PBMCs following 24 h of coculture with hk‐MCC1849. Phagocytosis of fluorescently labeled hk‐MCC1849 by pDCs was observed, and there were significant increases in CD86 (p < .05) and HLA‐DR (p < .0001) expression in pDCs. These results suggest that hk‐MCC1849 exerts a potential immunomodulatory effect on the host through the activation of peripheral pDCs.