bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of a Small-cell Lung Cancer (SCLC) Mouse Model Using Enhanced Cancer Stem Cell–functioning 3D SCLC Spheroids
Authors: Jung Yong Hun , Kim In Kyoung , Eom So Young , Kim Hyunsoo , Park Kyungwon , Chung Seok , Lee Sang Haak , Yeo Chang Dong , Kim Hye Joung , Kang Hye Seon 
Published: December 2023

Abstract
Despite its low incidence rate, small-cell lung cancer (SCLC), which accounts for approximately 10–15% of all lung cancers, is concerningly characterized by its rapid progression, strong metastatic potential, and high fatality rate. Therefore, an effective predictive model for SCLC treatment is imperative for early intervention. In this study, we aimed to enhance the cancer stem cell (CSC) capabilities of SCLC through a three-dimensional (3D) culturing approach and to evaluate its potential as a predictive model for therapeutic response in SCLC by transplanting the spheroids into mice. To achieve uniform and scalable production of 3D SCLC spheroids, the SpheroFILM culture platform was employed, and the expression of CSC markers was verified at the gene and protein levels within the spheroids cultured onboard. Moreover, spheroids were transplanted into mice to confirm gene and protein expression of CSC markers, tumor-formation capacity, proliferation ability, and invasion potential through histological staining. Based on our results, the transplantation of enhanced CSC–functioning 3D SCLC cell lines into mice led to a significant increase in tumor-formation capacity along with elevated expression of CSC markers. Thus, the results collectively suggest that the augmentation of CSC properties in 3D cultured cell lines, when transplanted into animal models, reduces necrosis and enhances tumor formation, proliferation, and invasion.
Low HBV Knowledge is Associated with Low HBV Vaccination Uptake in General Adult Population Despite Incentivization of HBV Vaccination
Authors: Kim Thanh Van , Pham Trang Nguyen Doan , Do Brian , Dao Diem Vu Bich , Nguyen Dan Xuan , Lee William , Gish Robert , Mize Gary , Trang Amy , Le Anh , Phan Loc Thi Bich , Ngo Thi-Thuy-Dung , Phan Hai Thanh , Nguyen Binh Tan , Tang Hong Kim , Dao Doan Y 
Published: December 2023

Abstract

Background. Hepatitis B virus (HBV) vaccination in Vietnamese adults remains low and unequally distributed. We conducted a study on HBV-naïve adults living in Ho Chi Minh City, Viet Nam, to determine barriers associated with HBV vaccination uptake after removing the financial barrier by providing free coupons for HBV vaccination.
Methods. After being screened for HBsAg, anti-HBs, and anti-HBc, 284 HBV-naïve study participants aged 18 and over (i.e., negative for HBsAg, anti-HBs, and anti-HBc total) were distributed with free 3-dose HBV vaccine coupons. Next, study participants’ receipt of 1st, 2nd, and 3rd doses of HBV vaccine was documented at a pre-specified study healthcare facility, where HBV vaccines were distributed at no cost to the participants. Upon study entry, participants answered questionnaires on sociodemographics, knowledge of HBV and HBV vaccination, and related social and behavioral factors. The proportions of three doses of HBV vaccine uptake and their confidence intervals were analyzed. Associations of HBV vaccine initiation with exposures at study entry were evaluated using modified Poisson regression.
Results. 98.9% (281 of 284) of study participants had complete data and were included in the analysis. The proportion of obtaining the 1st, 2nd, and 3rd doses of HBV vaccine was 11.7% (95%CI 8.0-15.5%), 10.7% (95%CI 7.1-14.3%), and 8.9% (95%CI 5.6-12.2%), respectively. On the other hand, participants were more likely to initiate the 1st dose if they had adequate knowledge of transmission (adjusted relative risk [aRR] = 2.58, 95% Confidence Interval [95% CI] 1.12-5.92), adequate knowledge of severity (aRR = 6.75, 95%CI 3.38-13.48), and annual health-checking seeking behavior (aRR = 2.04, 95%CI 1.07-3.87).
Conclusion. We documented a low HBV vaccination uptake despite incentivization. However, increased vaccine initiation was associated with better HBV knowledge and annual health check-up adherence. When considering expanding HBV vaccination to the general adult population, we should appreciate that HBV knowledge is an independent predictor of vaccine uptake.
Frequency dependent model for bone remodeling stimulated by harmonic mechanical loading
Authors: Lu Yanfei 
Published: December 2023

Abstract
In this paper, the bone tissue was modeled as a linear viscoelastic material saturated with interstitial fluid. We considered a specific case of harmonic loading and related the mechanical stimuli to the loading frequency. In this way, we could include the inertial effect in the model while not having to deal with the perturbation during each loading period. Two types of mechanical signals were considered: strain energy and dissipation energy. A parametric study revealed the dependency of the two signals on loading frequency and material property. The evolution of the apparent mass density supported the parametric study's findings. Under the three different frequency loadings, the strain energy-stimulated samples experienced identical remodeling scenarios. The samples stimulated with dissipation energy, on the other hand, exhibited a strong frequency dependence. An additional study was performed to investigate the effect of long-term variations in the loading frequency on the remodeling process. This demonstrated the model's capabilities in designing and evaluating load regimes for rehabilitation following a bone injury or bone reconstruction.
Maize/soybeans intercropping increases nutrient uptake, crop yield and modifies soil physio-chemical characteristics and enzymatic activities in a subtropical humid region based in Southwest China
Authors: NASAR JAMAL , AHMAD MUNIR , Gitari Harun , Tang Li , Chen Yuan , Zhou Xun-Bo 
Published: December 2023

Abstract
In China, the high use of nitrogen fertilization for agricultural production and intensive mono-cropping systems have adversely affected the agricultural ecosystem and environment. Therefore, to improve the sustainable agricultural production system, farming systems need to be adjusted within the country. Consequently, a two-year (2021–2022) field experiment was conducted on the maize/soybean intercropping practices with/without nitrogen (N) fertilization (i.e., N0; 0 N kg ha− 1 and N1; 225 N kg ha− 1) to know whether such cropping system can improve the nutrients uptake and crop yields, soil physio-chemical properties, and soil enzymes, which ultimately results in enhanced crop yield. The results revealed that maize intercropping treatments (i.e., N0MI and N1MI) had higher crop yield, biomass dry matter, and 1000-grain weight of maize than mono-cropping treatments (N0MM, and N1MM). Nonetheless, these parameters were optimized in N1MI treatments in both years. For instance, N1MI produced the maximum grain yield (10105 and 11705 kg ha− 1), biomass dry matter (13893 and 14093 kg ha− 1), and 1000-grain weight (420. and 449 g) of maize in the year 2021 and 2022, respectively. Conversely, soybean intercropping treatments (i.e., N0SI and N1SI) reduced such yield parameters for soybean. Also, the land equivalent ratio (LER) and land equivalent ratio for N fertilization (LERN) values were always greater than 1, showing the intercropping system's benefits in terms of yield and improved resource usage. Moreover, maize intercropping treatments (i.e., N0MI and N1MI) and soybean intercropping treatments (i.e., N0SI and N1SI) significantly (p < 0.05) enhanced the nutrient uptake (i.e., N, Fe, P, K, Ca, and Zn) of maize and soybean, however, these nutrients uptakes were more prominent in N1MI and N1SI treatments of maize and soybean, respectively in both years (2021 and 2022) compared with their mono-cropping treatments. Similarly, maize-soybean intercropping treatments (i.e., N0MSI and N1MSI) significantly (p < 0.05) improved the soil-based N, K, P, NH4, NO3, and soil organic matter, but, reduced the soil pH. Such maize-soybean intercropping treatments also improved the soil enzymatic activities such as protease (PT), sucrose (SC), acid phosphatase (AP), urease (UE), and catalase (CT) activities. This indicates that maize-soybean intercropping could potentially contribute to higher and better crop yield, enhanced plant nutrient uptake, improved soil nutrient pool, physio-chemical characteristics, and related soil enzymatic activities. Thus, preferring intercropping to mono-cropping could be a preferable choice for ecological viable agricultural development.
Hydrological Droughts in the Eastern Mediterranean Basin Using Hybrid Trend Analysis Methods
Authors: Alashan Sadık , Gül Enes , Toprak Ahmet , İkincioğulları Erdinç 
Published: December 2023

Abstract
This study investigates the impact of climate change on river systems within the Eastern Mediterranean Basin (EMB), utilizing the Mann-Kendall (MK) test, enhanced by Sen’s slope estimator and Şen’s Innovative Trend Analysis (ITA) methods. The research focuses on hydrological changes in river systems, particularly streamflow trends, and their implications under climate change and anthropogenic activities. The methodology includes a detailed analysis of hydro-meteorological series, including streamflow data from different observation stations. To satisfy the serial independence requirement assumption of MK test pre-whitening and over-whitening methodologies are applied effectively. The study area comprises 10 sub-basins in the EMB, with particular attention to the Göksu River and its tributaries. The results clearly show significantly decreasing trends in the annual streamflow values at several stations, demonstrating the considerable influence of climate and environmental changes on the basin's hydrology. These trends are also critically analyzed with the help of ITA graphs, which provide insights into the spatial and temporal variability of streamflow patterns. This research contributes to a better understanding of hydrological responses to climatic variability, providing vital information for water resource management and policies in regions undergoing significant environmental changes.
Performance Study of Cold-formed steel Channel joist with web opening subjected to flexural loading
Authors: KSK. Duraimurugan , Rajaram Basker 
Published: December 2023

Abstract
This study presents the structural behaviour of cold-formed steel joists with different web-opening shapes. The investigation incorporated both experimental and analytical approaches. The study involved the selection of square and circular web openings, and channel sections were chosen based on site-specific design considerations. In the experimental phase, channel joists were subjected to flexural loading and tested. An analytical investigation was conducted. Finite element (FE) models were developed using the FEM software ANSYS APDL version 16.2. FE models were used to simulate the structural response of the channel joist when subjected to flexural loading. A parametric study was conducted on the basis of simulation and experimental investigation. Factors that influence stiffness and load-carrying capacity considering variables in cold-formed steel channel joists were analyzed. Analytical investigations were conducted. Based on this result analysis, significant effects were observed, indicating a better agreement between the analytical and experimental results. In addition, it was shown that channel joists with circular web openings provide high efficiency in terms of load-carrying capacity and stiffness in web-opening shapes.
Deletion of Slc9a1 in Cx3cr1+ cells stimulated microglial subcluster CREB1-BDNF signaling and microglia-oligodendrocyte crosstalk
Authors: Song Shanshan , Oft Helena , Metwally Shamseldin , Paruchuri Satya , Bielanin John , Fiesler Victoria , Sneiderman Chaim , Kohanbash Gary , Sun Dandan 
Published: December 2023

Abstract
Microglial Na/H exchanger-1 (NHE1) protein, encoded by Slc9a1, plays a role in white matter demyelination of ischemic stroke brains. To explore underlying mechanisms, we conducted single cell RNA-seq transcriptome analysis in conditional Slc9a1 knockout (cKO) and wild-type (WT) mouse white matter tissues at 3 days post-stroke. Compared to WT, Nhe1 cKO brains expanded a microglial subgroup with elevated transcription of white matter myelination genes including Spp1, Lgals3, Gpnmb, and Fabp5. This subgroup also exhibited more acidic pHi and significantly upregulated CREB signaling and BDNF secretion detected by ingenuity pathway analysis and flow cytometry. Moreover, the Nhe1 cKO white matter tissues showed enrichment of a corresponding oligodendrocyte subgroup, with pro-phagocytosis and lactate shuffling gene expression, where activated CREB signaling is a likely upstream regulator. These findings demonstrate that attenuation of NHE1-mediated H+ extrusion acidifies microglia and stimulates microglial CREB1-BDNF signaling, giving rise to restorative microglia-oligodendrocyte interactions to potentially improve post-stroke remyelination.
Enantiospecific Attraction of Lygus Lineolaris Palisot De Beauvois (Hemiptera: Miridae) to a Ubiquitous Floral Volatile in the Field
Authors: Hetherington Matthew C. , Weissner Morgan , Guédot Christelle 
Published: December 2023

Abstract
Herbivorous insects utilize olfactory and visual cues to orient on suitable host plants, and such cues can be employed to facilitate insect monitoring. Lygus lineolaris Palisot de Beauvois is a polyphagous pest throughout North America. Monitoring this pest as it moves between crop and non-crop hosts remains challenging and a lack of effective monitoring tools complicates management of this insect. In this study, we examined the electrophysiological and behavioral responses of L. lineolaris to the volatile emissions of two crop hosts: Medicago sativa and Fragaria ananassa. Gas chromatography with electroantennographic detection was applied to identify antennally active compounds in headspace extracts of flowering host plants, before responses to individual compounds were examined in the field. Five compounds consistently elicited antennal depolarizations in adult L. lineolaris and, of these, (±)-linalool increased female L. lineolaris capture rate in the field. Subsequent experiments examined the influence of visual cues and stereochemistry on capture rate, revealing that lures containing (±)-linalool and (S)-(+)-linalool significantly increased female L. lineolaris capture rates compared to traps baited with (R)-(-)-linalool and controls, indicating that L. lineolaris is attracted to (S)-(+)-linalool. While lures increased capture on red traps, this was not the case for white traps, emphasizing the importance of visual cues in L. lineolaris monitoring. This study demonstrates that L. lineolaris is attracted to (S)-(+)-linalool in the field, and that attraction depends on trap color. This knowledge is expected to improve L. lineolaris monitoring in agricultural systems.
Development of a Myeloid-Derived Suppressor Cells-Related Genes Predictive Signature for Prognostic Survival and Immunotherapeutic Responses in Hepatocellular Carcinoma
Authors: Zhao Minjie , Li Chunming , Gong Jianping 
Published: December 2023

Abstract
Background
In hepatocellular carcinoma (HCC), myeloid-derived suppressor cells (MDSCs) play a vital role in the tumor immune microenvironment, significantly impacting tumor initiation and progression. The study of genes linked to MDSCs in predicting the progression of HCC is of significant scholarly significance.
Method
Acquiring a comprehensive single cell RNA sequencing (scRNA-seq) dataset from GEO to extract valuable information and detect distinct cell subgroups through the application of clustering and dimensionality reduction techniques. Moreover, the determination of molecular subtypes that demonstrate excellent clustering performance is achieved by calculating the cumulative distribution function (CDF). The analysis of the immune landscape and tumor immune escape state is conducted in this study using estimation methods, particularly the cibersort algorithm, in conjunction with publicly accessible tools for tumor immune dysfunction and exclusion (TIDE). Furthermore, a Cox regression analysis is employed to establish a gene risk model associated with MDSCs, which is later confirmed using various datasets and dimensions. Moreover, a functional enrichment analysis is performed to detect potential signaling pathways linked to marker genes of MDSCs.
Result
From the scRNA-seq dataset (GSE202642), a collection of 30 subpopulations and 65 marker genes associated with MDSCs were discovered. When clustering the molecular subtypes using marker genes related to MDSCs, there were notable differences in prognostic survival and immune signatures observed between the two subtypes. As a result, an independent prognostic factor for patients with HCC was discovered, which is a 7-gene predictive signature consisting of DAB2, FTL, CSTB, APOE, PLA2G7, PPT1, and ANXA2. Individuals with a lower RiskScore exhibited a reduced survival rate and higher immune infiltration in contrast to patients who had a higher RiskScore.
Conclusion
In summary, a highly effective MDSCs-related marker was created to predict the prognosis and response to immunotherapy in individuals diagnosed with HCC.
An algorithm for English capital letters based on discrete Hopfield network and BP network
Authors: Wan Xiaojuan , Yang Yongxin , Bai YanPing , Xie Congjin , Liu XiaoLin , Du Bingfang 
Published: December 2023

Abstract
English is the most widely used language in the world. In reality, we often need to get some old English newspapers or books and periodicals information, even the license plate also has English letters, these important English information often takes the form of English capital letters. But because of the age, or other factors, these English letters are often not very clear. The accuracy of English letter recognition at a certain level determines whether English handwriting can be processed quickly and efficiently by a computer. The Hopfield network is a recognized network of associative memory patterns. How to maximize its associative recognition ability and efficiently recognize handwritten English letters is the main focus of this article. This paper analyzes various problems encountered in the actual processing process and explores the performance of discrete Hopfield. To verify the identification ability of another network, Hopfield was added. That is, the recognition result of DNHH is used as input for BP network, and then identified by BP network. Compare with only the same noise directly with BP network identification. According to the experimental results, Hopfield does have a certain recognition effect. Clearly improve the accuracy of the recognition.
Molecular Dynamics, Inhibitive, Structural Activity, and Toxicity Studies of Novel 4-aminoquinoline Imidazole Analogues Against Chloroquine-Resistant-Plasmodium falciparum (3D7 strain)
Authors: Salau Shina , Ahmed Sikiru Akinyeye , Saliu Taofeek , Ahmed Rabiat Shola , Suleiman Nafisat Omowumi 
Published: December 2023

Abstract
Chloroquine resistance in Plasmodium falciparum, is the deadliest form of malaria. It is associated with several mutations of PfCRT (P. falciparum chloroquine resistant transporter): PfCRT is a protein group that serves as a transporter in the parasite's digesting vacuole membrane. In the quest to battle chloroquine-resistant—P. falciparum strains, this study aim to predict the anti-plasmodial activity of ten (10) novel 4-aminoquinoline imidazole (4AQI) analogues (new-sets), whose activity was modeled using a multi-linear regression algorithm; trained by the structural-activity data from fifteen (15) 4-aminoquinoline (4aq). Additionally, the study aim to understand the effect of electron delocalization and the extent to which the structural affinity of the new-sets has to the Pf3D7 protein. Results reveal that R2 value (0.9180), R2pred value (0.8276), and Q2 value (0.6672) obtained from the QSAR studies proofed to be sufficient for constructing and testing the QSAR model. According to molecular docking studies, it was understood that new-sets with electron withdrawing groups, such as, -CO2H, -NO2, -OH, F, Cl, and Br have a higher tendency toward plasmodium affinity than those with electron donating groups like -OCH3 and -NH2 against the Pf3D7 protein. All ten (10) 4AQI (new-sets) were approved as prospective drugs by the ADMET predictive study. Molecular dynamics results simulation at 50ns of time reveals that of all the new-sets, n16 and n21 stabilized the Pf3D7 (~ 0.1–0.2 Å) the most and better than chloroquine with respect to the protein structure. Furthermore, n16, n21, and chloroquine could provide flexibility in two amino acid regions 180–230 (~ 0.95–1.55Å) and 260–289 (~ 0.7–1.2Å). Additionally, n16 and n21 complexes showed retained a stable range of 210–250 number of hydrogen bonds, while chloroquine maintained a range of 180–220 hydrogen bonds.
The research of oil washing method for Oil-based mud cuttings samples from exploration wells
Authors: Kui Chen , Shikui Zhai , Yushuang Zhu , Peiyuan Zhu , Jinlai Huan , Chun Xiu 
Published: December 2023

Abstract
In order to eliminate the pollution of oil-based mud to deep-water drilling cuttings in Qiongdongnan Basin, South China Sea, and ensure the smooth development of drilling cuttings logging, oil-gas identification and subsequent analysis and testing, an exploratory study on the experimental method of mud-based oil cleaning was carried out, in which a Soxhlet extractor with siphon effect was selected as the oil washing equipment, and anhydrous ethanol and carbon tetrachloride as the organic reagents for oil washing. Get the following understanding: Through the analysis of the shortcomings of several exploratory experiments of ' wash clean point baseline method ', several key points of the experiment were implemented, and two methods of ' continuous sampling washing oil method ' and ' equal-division oil washing method ' were summarized. When the sample was washed for 20.5h, the base oil in the sample had been completely removed, which guided the subsequent batch washing experiment.
Research focusing on Junjube's storage and preservation techniques in Xinjiang in connection with the Internet of Things
Authors: Zhang Bin , Lin Xiao Dan , zhang Xue Min , Ma Shao Hui , Ding Yu 
Published: December 2023

Abstract
The surge in jujube yield has left numerous farmers baffled about maintaining the freshness of these jubes. To tackle this issue, we've developed a jujube storage system that integrates Internet of Things technology. The system incorporates a programmable controller (PLC) as its core control, utilizes HMI for human-computer interaction, and sets up the system's equipment, transmission, and application layers. In collaboration with the Internet of Things cloud platform, this system utilizes TCP/IP protocol and the 4G network for transmitting data, easing the data exchange between the equipment layer and the cloud server, and managing the system's remote control via the cell phone applet. Data indicates the system's robustness, improving jujube's automated preservation and freshness, prolonging its shelf life and quality, and establishing it as a standard for intelligent fruit and vegetable storage.