bluuebunny/crossref_metadata_2025_split huggingface dataset:
Questões agrárias: agricultura familiar, acesso à terra e produção para autoconsumo
Authors: Santos Leiliane Rosa de Jesus , Araújo Vanessa Marzano 
Published: January 2025

A terra é o principal meio para manter o agricultor no campo e, consequentemente, viabilizar a prática da agricultura familiar que permite acesso aos alimentos em quantidade e qualidade para que eles possam se manter nesse espaço sem, necessariamente, possuir uma renda monetária de forma constante. Esse artigo analisa o acesso à terra e a produção de autoconsumo em um assentamento rural no município de Jequitinhonha, MG. A pesquisa iniciou-se com um estudo bibliográfico, na sequência ocorreu pesquisa de campo, entrevistando as cinquenta e sete famílias assentadas. Os resultados mostraram que o povoamento da região se deu de forma expansiva e conflituosa. Conclui-se então que o acesso à terra foi fundamental para que as famílias produzissem alimentos saudáveis e variados, mas que devido à falta de água em algumas épocas do ano não conseguem produzir em grande quantidade, devendo se ajustar a venda dos excedentes na feira para compra de outros produtos.
Dawn, Mortals and Immortals in the Aeneid
Authors: Cianciosi Stefano 
Published: January 2025

Abstract
In Homer, Aurora sometimes illuminates gods and men. In Virgilian Dawn scenes, however, the gods are never said to receive her light. Thematic reasons can explain why this is the case. But even the intellectual progress of the centuries that separated Virgil from the Homeric poems might have influenced his choice. Ancient Homeric scholarship questioned the plausibility of the gods’ need for sunlight, and Hellenistic and Roman poets who influenced Virgil had already omitted the immortals in these formulae. The depiction of the gods in the Aeneid could respond to numerous factors such as the mechanics of the poet’s technique as well as aesthetic, philosophical and scientific trends that left traces in the writings of ancient Homeric scholars and imitators. Considering all these factors allows for a more nuanced understanding of Virgil’s modus operandi and can bring us closer to the perspective of a Roman audience of his time.
Developing active learning activities for didactic perfusion courses
Authors: Kim Catherine , Gerrits Ronald 
Published: January 2025

Background In the world of academia, traditional lecturing has been the most common pedagogical approach for centuries. However, it can create an environment for students to be passive learners in the classroom. Alternatively, active learning is a pedagogical approach intended to encourage students to engage with content in manners which have been associated with improved exam performance, final course grades, clinical reasoning skills, and critical thinking skills. Given that perfusion is inherently a profession where didactic information must be applied in practice, the implementation of active learning in didactic courses is worth considering. Although active learning is practiced in perfusion education through participation in simulations and clinical rotations, there are no easily identifiable active learning resources available to supplement didactic perfusion courses. In response, written active learning activities were specifically designed for didactic perfusion courses. Methods The activities were designed based on the format for guided inquiry worksheets, the incorporation of the learning cycle, and the implementation of process oriented guided inquiry learning (POGIL) strategies. Key concepts were identified, learning objectives were specified, and models (figures or tables of data) were created as targets of engagement and analysis. Following each model, questions and prompts are given to guide students through identifying, understanding, and exploring the concepts incorporated in the activity as well as opportunities to apply the newly gained knowledge and understanding. Results Ten active learning activities have been developed based on the methods summarized above. Conclusion While the purposes of this paper are to inform the perfusion community of active learning and to share guidelines on how to create active learning activities for didactic perfusion courses, the goals of the activities are to provide examples to perfusion educators and to guide students in understanding basic perfusion concepts in a clinically relevant manner. It is recommended that perfusion students complete these activities alongside their didactic perfusion courses.
Mapping the regional and remote specialised mental health workforce: Commentary on the AIHW data for 2022–2023
Authors: Looi Jeffrey CL , Allison Stephen , Bastiampillai Tarun , Kisely Steve 
Published: January 2025

Objective The Australian Institute of Health and Welfare publishes statistical indicator reports on the specialised mental health workforce. These include data for 2022–2023 on psychiatrists, mental health nurses, mental health occupational therapists, psychologists and mental health social workers. We provide a brief commentary on these reports, reflecting upon the implications of such changes for psychiatric practice and patient care. Conclusions Overall, there are fewer mental health workers with increasing distance from urban centres. There are insufficient rural psychiatrists with the NT and Queensland having higher rates per 100,000 in outer regional and remote areas. Psychologists and mental health nurses have the highest rates per 100,000 in rural areas. Though low in absolute rates per 100,000, mental health social workers are better distributed in rural compared to urban areas. Further data on public, private and non-governmental sector employment would be useful.
Sustainable sound absorption using shredded plastic particles: Adjusting low-frequency acoustic performance with particle size
Authors: Železnik Anže , Čurović Luka , Prezelj Jurij 
Published: January 2025

This study investigates the sound absorption properties of shredded recycled plastic particles, with a focus on how particle size influences low-frequency acoustic performance. Given the growing environmental challenge posed by plastic waste, this research aims to repurpose such materials into effective, sustainable sound absorbers, contributing to both noise reduction and waste minimization efforts. The sound absorption coefficients and normalized surface impedances of different size fractions, ranging from 45 µm to 3 mm, were measured using the standard two-microphone impedance tube method in accordance with ISO 10534-2. The analysis revealed that smaller particle sizes significantly enhance low-frequency absorption, though at the expense of the maximum absorption coefficient across a broader frequency range. This trade-off underscores the importance of particle size in designing materials tailored to specific acoustic applications. Results indicate that optimal sound absorption shifts towards higher frequencies for larger particle sizes, while finer particles are more effective at lower frequencies. The ability to customize the acoustic properties of shredded plastic particles by selecting appropriate particle sizes is a key outcome of this study. Microscopic examination of the particles revealed a correlation between particle morphology and acoustic performance, with irregular shapes contributing to enhanced sound energy dissipation. The findings suggest that shredded recycled plastic particles are a viable and sustainable option for creating customizable sound-absorbing materials, particularly in applications where low-frequency noise reduction is critical. This research not only advances the understanding of acoustic performance in recycled materials but also supports broader sustainability goals by offering an innovative use for plastic waste in industrial and architectural noise control solutions. In conclusion, this study provides a foundation for the development of eco-friendly, effective sound-absorbing materials from recycled plastics, emphasizing the importance of particle size in achieving desired acoustic properties.
Ensifentrine in COPD patients taking long-acting bronchodilators: A pooled post-hoc analysis of the ENHANCE-1/2 studies
Authors: Dransfield Mark , Marchetti Nathaniel , Kalhan Ravi , Reyner Daniel , Dixon Amy L , Rheault Tara , Rickard Kathleen Ann , Anzueto Antonio 
Published: January 2025

Background The efficacy and safety of ensifentrine, a novel PDE3/PDE4 inhibitor, were previously evaluated in the ENHANCE-1 (NCT04535986) and ENHANCE-2 (NCT04542057) trials. Here, we present a pooled post-hoc subgroup analysis of patients according to background chronic obstructive pulmonary disease (COPD) maintenance medication regimens. Objective This analysis aimed to explore the efficacy and safety of ensifentrine in patients receiving long-acting muscarinic antagonists (LAMA) or long-acting beta-agonists with inhaled corticosteroids (LABA + ICS). Methods Eligible patients had moderate to severe COPD, were aged 40–80 years, and were symptomatic at randomization. Patients were randomized 5:3, receiving twice-daily ensifentrine 3 mg or placebo via standard jet nebulizer over 24 weeks. Results The pooled post-hoc analysis included 485 LAMA patients and 272 LABA + ICS patients. Ensifentrine showed lung function improvement over placebo at week 12, including average FEV1 AUC0–12 h in the LAMA (placebo-corrected least squares mean change from baseline [LSMC], 92 mL; 95% CI, 54, 131; p < 0.001) and LABA + ICS subgroups (LSMC, 74 mL; 95% CI, 27, 121; p = 0.002). Ensifentrine reduced the rate and risk of exacerbations in both LAMA (48% and 50%, respectively) and LABA + ICS (51% and 56%, respectively) subgroups. Ensifentrine-treated patients reported improvement in symptoms and quality of life over 24 weeks. The safety profile of ensifentrine in each subgroup was similar to the profile in the pooled modified intention-to-treat population. Conclusions Nebulized ensifentrine offers a novel non-steroidal anti-inflammatory and bronchodilator treatment added to existing LAMA or LABA + ICS treatment options in patients with moderate to severe, symptomatic COPD.
Omega-3 fatty acids: molecular weapons against chemoresistance in breast cancer
Authors: Marchio Vittoria , Augimeri Giuseppina , Morelli Catia , Vivacqua Adele , Giordano Cinzia , Catalano Stefania , Sisci Diego , Barone Ines , Bonofiglio Daniela 
Published: January 2025

Abstract
Breast cancer is the most commonly diagnosed type of cancer and the leading cause of cancer-related death in women worldwide. Highly targeted therapies have been developed for different subtypes of breast cancer, including hormone receptor (HR)-positive and human epidermal growth factor receptor 2 (HER2)-positive breast cancer. However, triple-negative breast cancer (TNBC) and metastatic breast cancer disease are primarily treated with chemotherapy, which improves disease-free and overall survival, but does not offer a curative solution for these aggressive forms of breast cancer. Moreover, the development of chemoresistance is a major cause of therapeutic failure in this neoplasia, leading to disease relapse and patient death. In addition, chemotherapy’s adverse side effects may substantially worsen health-related quality of life. Therefore, to improve the outcome of patients with breast cancer who are undergoing chemotherapy, several therapeutic options are under investigation, including the combination of chemotherapeutic drugs with natural compounds. Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs), including docosahexaenoic and eicosapentaenoic acids, have drawn attention for their antitumoral properties and their preventive activities against chemotherapy-induced toxicities in breast cancer. A literature review was conducted on PubMed using keywords related to breast cancer, omega-3, chemoresistance, and chemotherapy. This review aims to provide an overview of the molecular mechanisms driving breast cancer chemoresistance, focusing on the role of ω-3 PUFAs in these recognized cellular paths and presenting current findings on the effects of ω-3 PUFAs combined with chemotherapeutic drugs in breast cancer management.
Crop protection by RNA interference: a review of recent approaches, current state of developments and use as of 2013
Authors: Germing Kirsten , Navarrete Cecilia Andrea Díaz , Schiermeyer Andreas , Hommen Udo , Zühl Luise , Eilebrecht Sebastian , Eilebrecht Elke 
Published: January 2025

Abstract
RNAi-based applications aim to inhibit the expression of specific essential genes in target organisms by uptake and processing of double-stranded RNA and subsequent degradation of target gene mRNA. While the research on RNAi-based pesticides was initially limited to endogenous applications (i.e., production of the dsRNA by the crop), the development of technologies for exogenous applications like RNAi sprays in particular has increased in recent years. Due to the assumed beneficial properties of RNA molecules, such as degradability or target specificity, RNAi technology receives increasing attention in the development of plant protection products, as evidenced by a steadily increasing number of publications. A horizon scan was conducted with a specific emphasis to identify and illustrate the current state of RNAi developments and applications in crop protection as well as their biomanufacturing readiness levels. In this study, more than 180 publications were evaluated. Furthermore, we identified 268 patent families on this topic. A large variety with regard to treated crops, targeted pest species and target gene functions as well as application types was observed. In total, RNAi applications for more than 30 different pest species were identified, most of which belonged to the insect orders Lepidoptera, Hemiptera and Coleoptera. In addition, applications targeting fungi and viruses were found. RNAi applications were identified to be an upcoming topic in crop protection and, therefore, are becoming increasingly relevant for environmental risk assessment, due to the high number of targeted pest species as well as the variety of novel application types. With this review, we inform future work aimed to develop further adequate concepts and methods for environmental risk assessment of RNAi-based applications.
Transcriptional time-course analysis during ash dieback infection revealed different responses in tolerant and susceptible Fraxinus excelsior genotypes
Authors: Chano Víctor , Ferrari Renata Callegari , Domínguez-Flores Tania , Shrestha Karuna , Fussi Barbara , Seidel Hannes , Gailing Oliver , Budde Katharina B. 
Published: January 2025

Abstract

Hymenoscyphus fraxineus, the causal agent of Ash Dieback (ADB), has been introduced to eastern Europe in the 1990s from where it spread causing decline in European ash populations. However, the genetic basis of the molecular response in tolerant and susceptible ash trees to this disease is still largely unknown. We performed RNA-sequencing to study the transcriptomic response to the disease in four ash genotypes (ADB-tolerant FAR3 and FS36, and ADB-susceptible UW1 and UW2), during a time-course of 7, 14, 21, and 28 days post-inoculation, including mock-inoculated trees as control samples for each sampling time point. The analysis yielded 395 and 500 Differentially Expressed Genes (DEGs) along the response for ADB-tolerant FAR3 and FS36, respectively, while ADB-susceptible UW1 and UW2 revealed 194 and 571 DEGs, respectively, with most DEGs found exclusively in just one of the genotypes. DEGs shared between tolerant genotypes FAR3 and FS36, included genes involved in the production of phytoalexins and other secondary metabolites with roles in plant defense. Moreover, we identified an earlier expression of genes involved in both pattern- and effector-triggered immunity (PTI and ETI) in ADB-tolerant genotypes, while in ADB-susceptible genotypes both responses were delayed (late response). Overall, these results revealed different transcriptomic expression patterns not only between ADB-tolerant and ADB-susceptible genotypes, but also within these two groups. This hints to individual responses in the natural tolerance to ADB, possibly revealing diversified strategies across ash genotypes.
Reticulocyte hemoglobin content: a new frontier in iron deficiency diagnostics for major surgical patients
Authors: Choorapoikayil Suma , Kotlyar Mischa J. , Kawohl Lisa , Pratz Paul P. , Mehic Denana , Kranke Peter , Rumpf Florian , Blum Lea V. , Kloka Jan A. , Zacharowski Kai , Neef Vanessa , Meybohm Patrick 
Published: January 2025

Abstract

Background
Iron deficiency (ID) is the most common nutritional deficiency among patients undergoing major surgery. Treatment of ID is straightforward, however implementing a comprehensive anemia management strategy within clinical routines is complex. Recently, reticulocyte hemoglobin content (Ret-He) has been evaluated as an early marker for ID diagnosis.

Method
In this retrospective study, 2,966 major surgical patients from two University Hospitals were screened for the presence of ID and the significance of Ret-He in diagnosis ID was evaluated in both non-anemic and anemic patients. According to hemoglobin, ferritin, and transferrin saturation concentrations patients were assigned to a Control group (no anemia, no ID), ID group (no anemia, ID), IDA group (anemia, ID) or Others group (anemia, no ID).

Results
In total, 2,760 patients were included in analysis: Control (n = 1500; 54.2%), IDA (n = 412; 14.9%), ID (n = 487; 17.6%), and Others (n = 370; 13.4%). Ret-He was significantly decreased in the IDA group compared to ID, Control and Others, respectively (p < 0.001). The ROC curve analysis revealed an AUC of 0.842 (95% CI (0.82–0.87)) at Ret-He cutoff 33.5 pg, by which IDA was discriminated with 69.7% (95% CI (65.3–74.0%)) sensitivity and 85.7% (95% CI (82.3-86.1%)) specificity. Of the 370 patients with anemia of unknown cause (Others group) 131 had Ret-He levels < 33.5 pg. In these patients, the median values for ferritin was 492.0 ng/ml (333.5; 818.5 ng/ml) and transferrin saturation 11.9% (18.0; 23.3%). Logistic regression identified significant predictors of ID, with each decrease in Hb and Ret-He associated with a 19.4% (OR = 0.806; p < 0.001) and 26% (OR = 0.740; p < 0.001) increase in the odds of ID, respectively.

Conclusion
This study highlights the potential of Ret-He as a promising alternative marker for diagnosing ID in patients undergoing major surgery, particularly in cases of elevated ferritin levels or non-anemic patients. Ret-He may serve as a valuable tool to prioritize patients for further iron status testing, especially when preoperative time is limited.
Application of artificial intelligence in nursing practice: a qualitative study of Jordanian nurses’ perspectives
Authors: Almagharbeh Wesam Taher , Alfanash Hazem AbdulKareem , Alnawafleh Khaldoon Aied , Alasmari Amal Ali , Alsaraireh Faris Abdelkarim , Dreidi Mutaz M. , Nashwan Abdulqadir J. 
Published: January 2025

Abstract

Background
Artificial Intelligence (AI) is increasingly applied in healthcare to boost productivity, reduce administrative workloads, and improve patient outcomes. In nursing, AI offers both opportunities and challenges. This study explores nurses’ perspectives on implementing AI in nursing practice within the context of Jordan, focusing on the perceived benefits and concerns related to its integration.

Method
A qualitative research approach was employed, involving semi-structured interviews with 25 nurses and 3 focus group discussions, each consisting of 7–8 participants. The data collected was coded and analyzed using thematic analysis to identify recurring patterns and key themes in the nurses’ views on AI.

Results
Three major themes emerged from the analysis: (1) AI as an efficiency tool – Nurses recognized AI’s ability to reduce administrative burdens and improve patient monitoring in real-time. (2) Ethical and practical concerns – Nurses raised issues regarding patient privacy, data security, and the fear that AI might replace human decision-making in care. (3) Lack of preparedness and training – There was a consensus on nurses’ inadequate training in AI tools, limiting their ability to integrate AI into their practice fully.

Conclusion
While AI is seen as a valuable tool to enhance nursing productivity, several challenges still need to be addressed, particularly regarding ethical concerns and insufficient training. To ensure AI complements nursing without compromising the human element, healthcare institutions must address these issues by implementing comprehensive training programs and establishing clear ethical guidelines.
Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) and mortality among survivors of liver cirrhosis: a prospective cohort study
Authors: Fotros Danial , Hekmatdoost Azita , Pashayee-khamene Fereshteh , Karimi Sara , Ahmadzadeh Saleheh , Saberifiroozi Mehdi , Hatami Behzad , Yari Zahra 
Published: January 2025

Abstract

Background
Cirrhosis is a medical condition marked by persistent liver damage, which leads to the development of fibrous tissue and compromised liver function. In the present study, we decided to investigate the possibility of a connection between the consumption of fermentable olig-, di-, monosaccharides, and polyols (FODMAPs) and mortality rates in cirrhotic patients by utilizing data obtained from a prospective cohort study.

Methods
This cohort study enrolled 166 ambulatory patients from two hospitals in Tehran, Iran, between 2016 and 2018, and followed them up for 5 48 months until April 30, 2022. During the 3,955 person-months of follow-up, 43 fatalities were recorded (36 men and 7 women). The study classified participants into three groups based on their FODMAPs consumption and assessed the risk of mortality using Cox proportional hazards regression models.

Results
Total FODMAPs intake was associated with increased overall mortality risk (T3 vs. T1, HR = 3.5; 95%CI: 1.05, 11.7; P-trend = 0.036). This significant trend was also observed for total fructans (T3 vs. T1, HR = 5.15; 95% CI: 1.15, 23.2; P-trend = 0.006) and fructose (T3 vs. T1, HR = 5.55; 95% CI: 0.54, 57.14; P-trend = 0.018). Mortality risk was U-shaped with galactooligosaccharide intake, a lower mortality risk was observed with lactose intake and a higher mortality risk with polyols intake, although these associations did not reach statistical significance.

Conclusion
In conclusion, this study highlights a higher risk of mortality with higher intake of fructans, excess fructose and total FODMAPs.