bluuebunny/crossref_metadata_2025_split huggingface dataset:
Specific IgE and IgG to gelatin in children with systemic cutaneous reactions to Japanese encephalitis vaccines
Authors: Sakaguchi M. , Miyazawa H. , Inouye S. 
Published: March 2003

Background: Systemic allergic reactions to Japanese encephalitis (JE) vaccine that include urticaria, angioedema, and rash have been reported. In Japan, children who suffered from allergic immediate‐type reactions to JE vaccine had antigelatin IgE in their sera. However, the immunologic mechanism of allergic nonimmediate‐type reactions that consist of cutaneous signs appearing several hours or more after JE vaccination has not been defined. Methods: Serum samples were taken from 28 children who showed allergic nonimmediate‐type cutaneous reactions to JE vaccine. Furthermore, serum samples were taken from 10 children who showed allergic immediate‐type reactions with cutaneous signs and/or respiratory symptoms to JE vaccine. We have defined an immediate‐type reaction as one occurring within 1 h after vaccination. Results: Of 10 children who showed immediate‐type reactions, all had antigelatin IgE and IgG. Of 28 children who showed systemic nonimmediate‐type reactions, one had antigelatin IgE and nine (32%) had antigelatin IgG. The child who had antigelatin IgE showed urticaria 2 h after JE vaccination. Conclusions: These results suggest that some children who showed allergic nonimmediate‐type reactions to JE vaccine were sensitized to gelatin.
Comparison of ELISA for tissue transglutaminase autoantibodies with antiendomysium antibodies in pediatric and adult patients with celiac disease
Authors: Salmaso C. , Ocmant A. , Pesce G. , Altrinetti V. , Montagna P. , Descalzi D. , Martino S. , Bagnasco M. , Mascart F. 
Published: March 2003

Background: Tissue transglutaminase (t‐TG) is the main autoantigen recognized by the endomysium antibodies (EMA) observed in patients with celiac disease (CD). The aim of the study was to assess an ELISA method for t‐TG antibodies (t‐TGA) with respect to EMA IF assay in pediatric and adult patients. Methods: t‐TGA were analyzed by ELISA in 220 sera samples: 82 patients with biopsy‐proven untreated CD (23 adults and 59 children), 14 CD children on gluten‐free diet, 18 asymptomatic relatives of CD patients, and 106 age‐matched control patients with gluten‐unrelated gastrointestinal diseases (58 adults and 48 children). Serum IgA EMA were tested on umbilical cord sections in all patients. Results: The great majority (92.7%) of untreated CD patients (both adults and children) were t‐TGA positive (values ranging from 20.1 to >300 AU). None of the child control patients and only two out of 58 (3.4%) of the adults with unrelated gastrointestinal diseases had serum t‐TGA positivity; two out of 18 first‐degree relatives with biopsy‐proved silent CD were t‐TGA (as well as EMA) positive. Finally, two out of 14 CD children, assuming a gluten‐free diet, had serum t‐TGA (as well as EMA). A highly significant correlation (P<0.001) was observed between t‐TGA concentrations and EMA. t‐TGA showed a sensitivity of 87% and 95%, a specificity of 97% and 100% for adults and children, respectively. Conclusions: The method is highly sensitive and specific in the diagnosis of CD and is promising as a tool for routine diagnostic use and population screening, especially in children.
Occupational contact urticaria from diglycidyl ether of bisphenol A epoxy resin
Authors: Kanerva L. , Pelttari M. , Jolanki R. , Alanko K. , Estlander T. , Suhonen R. 
Published: March 2003

Background: Epoxy resin (ER) is a common cause of occupational allergic contact dermatitis (ACD), but contact urticaria from ER is very rare.Methods and Results: A plastic‐product worker first developed ACD from diglycidyl ether of bisphenol A (DGEBA) epoxy resin, and subsequent exposure resulted half a year later in contact urticaria: first with edema of the lips and eyelids, and later an urticarial reaction on the upper chest, with strong swelling of the eyelids and tightness of the throat. The diagnosis was based on a positive skin prick test to his “own” ER compound, a positive prick test reaction to DGEBA, and a positive skin provocation test with the ER compound and DGEBA. The contact urticaria test reaction was strongly aggravated when the allergen was wiped off with an alcohol solution, apparently because the solution enhanced the penetration of the allergen.Conclusions: Our case is of interest, first, because contact urticaria from ER is very rare, and second, because this is the second report in which a strongly intensified contact urticaria reaction was provoked by an alcohol solution. We suggest that if the contact urticaria provocation test with low‐molecular‐weight chemicals is negative, a contact urticaria provocation test with alcohol (CUPTA) should be performed.
Influence of food processing on the allergenicity of celery: DBPCFC with celery spice and cooked celery in patients with celery allergy
Authors: Ballmer‐Weber B. K. , Hoffmann A. , Wüthrich B. , Lüttkopf D. , Pompei C. , Wangorsch A. , Kästner M. , Vieths S. 
Published: March 2003

Background: Celery root is often consumed in a processed form as a cooked vegetable or as a spice. So far, however, there has been no information about the allergenicity of processed celery in celery‐allergic patients. Methods: In 12 patients with a history of allergic reactions to raw or raw and cooked celery, double‐blind placebo‐controlled food challenges (DBPCFCs) with raw celery (n = 10), cooked celery (110°C/15 min; n = 11), and celery spice (n = 5) were performed. Nine patients underwent an open mucosal challenge with four samples of canned celery retorted at Co‐values (cooking effect) of 7.45–76.07 (corresponding to the time periods in minutes at a thermal influence of 100°C). IgE immunoblot analysis of celery extract was performed with sera of all challenged patients. The thermal stability of celery allergen was investigated by enzyme allergosorbent test (EAST) inhibition. Furthermore, intraperitoneal immunization of mice followed by a rat basophil leukemia (RBL) cell mediator release assay was used as a biological in vitro model to assess the allergenicity of processed celery. Results: Six out of 11 patients showed a positive DBPCFC to cooked celery and five out of five patients to celery spice. Allergenicity of celery was preserved in four patients with a positive DBPCFC to cooked celery even if celery was treated at a Co‐value of 76.07. Patients with positive DBPCFC to cooked celery reacted to known celery allergens (Api g 1, Api g 4, cross‐reactive carbohydrate determinants CCD). EAST inhibition showed that heat resistance of celery allergens decreases in the following order: CCD > Api g 4 > Api g 1. Accordingly, five of six patients with a positive DBPCFC to cooked celery were sensitized to profilin and/or CCD. The murine model reflected the reactivity of patients sensitized to the major allergen Api g 1. Conclusions: 1) In a subset of patients with a positive DBPCFC to cooked celery, celery remains allergenic even after extended thermal treatment (76.07 min/100°C). 2) Celery spice is allergenic for patients with an allergy to raw celery. 3) RBL cells sensitized with mouse IgE to raw celery may serve as a useful tool for screening the potential allergenicity of heat‐processed products containing celery.
Prick tests to aeroallergens: is it possible simply to wipe the device between tests?
Authors: Piette V. , Bourret E. , Bousquet J. , Demoly P. 
Published: March 2003

Background: Improperly performed skin prick tests (SPT) can lead to wrong allergy diagnosis and incorrect treatment. To overcome false‐positive results it is recommended to change the puncture device between each test, although very few studies have examined the real drawbacks (false‐positives) and advantages (time and cost savings) of using only one device.Methods: Two groups of 20 patients with rhinitis or asthma, sensitized to either house‐dust mites or grass pollens, had successive serial SPT to 9% codeine phosphate and the relevant allergen using the same needle or lancet, wiped between each test.Results: With both the needle and the lancet, there were 12.5–67.5% false‐positive results using the house‐dust mite or grass pollen allergen extracts, respectively. There were no false‐positive results with the 9% codeine phosphate.Conclusions: Our study shows that this technique is not reliable as it provoked an unacceptable number of false‐positive results.
Urinary leukotriene levels are increased during exacerbation of atopic eczema/dermatitis syndrome. Relation to clinical status
Authors: Adamek‐Guzik T. , Guzik T. J. , Czerniawska‐Mysik G. , Korpanty G. , Mastalerz L. , Radwan J. , Szczeklik A. 
Published: March 2003

Background:  Leukotrienes are potent mediators of allergic inflammation and their role in the pathogenesis of allergic disorders, particularly asthma, is well established. Their importance in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS) is still unclear. We aimed to compare urinary cysteinyl leukotriene (Cys‐LT) levels during exacerbation and remission of AEDS in relation to clinical status, IgE levels, and eosinophil counts. Methods:  Urinary Cys‐LTs were measured by direct enzyme immunoassay in 17 adult patients with AEDS and in 17 healthy controls in whom atopy had been excluded. Cys‐LTs were compared during exacerbation and remission of AEDS in relation to the clinical status measured by SCORAD. Total IgE levels were measured by enzyme‐linked immunoassay (ELISA). Results:
Mean clinical score during the exacerbation was 64.3 ± 3.1 and during remission 22.4 ± 4 (
P
< 0.01). Cys‐LTs levels were significantly higher during the exacerbation of AEDS than in the control group (230.9 ± 20.8 vs 123.2 ± 9.9 pg/mg creatinine;
P
< 0.005). During the remission, the difference between AEDS patients and the control group was not significant (96.3 ± 8.7
vs
123.2 ± 9.9 pg/mg creatinine;
P
= 0.8). During AEDS exacerbation Cys‐LTs levels were significantly correlated with the clinical status (
r
S
= 0.73,
P
< 0.01) and with eosinophil counts (
r
= 0.86;
P  < 0.01) but not with the duration of the disease, age of patients, or IgE levels. Conclusions:  Our results point to enhanced biosynthesis of Cys‐LTs during the AEDS exacerbations. Inflammatory cells, e.g. eosinophils are the most probable source of Cys‐LTs. A strong correlation between Cys‐LT levels and clinical status may in part explain preliminary clinical observations of efficacy of leukotriene antagonists in alleviating symptoms of AEDS.
The 3 mm skin prick test (SPT) threshold criterion is not reliable for Tyrophagus putrescentiae: the re‐evaluation of SPT criterion to dust mites
Authors: Kanceljak‐Macan B. , Macan J. , Plavec D. , Klepac T. , Milković‐Kraus S. 
Published: March 2003

Background: The mean wheal diameter ≥ 3 mm is the usual criterion for positive skin prick test (SPT) reaction to dust mites. The study assessed the accuracy of this SPT criterion with respect to specific IgE values of above 0.35 kUA/l (+ sIgE).Methods: Specific IgE (ImmunoCAP, Pharmacia AB Diagnostics, Uppsala, Sweden) and standard SPT to Dermatophagoides pteronyssinus (DP) and farinae (DF), Lepidoglyphus destructor (LD) and Tyrophagus putrescentiae (TP) (ALK, Hørsholm, Denmark) were performed in a random sample of 457 subjects, of whom 273 men (mean age 35.3 ± 11.0 years) and 184 women (mean age 37.9 ± 9.5 years). Statistical analysis was performed using the chi‐square test, regression analysis and discriminant analysis.Results: When the mean wheal diameter of ≥ 3 mm was considered positive (+ SPT), the correlation between + SPT and + sIgE was 0.47 for DP (P < 0.001), 0.43 for DF (P = 0.004), 0.35 for LD (P = 0.03) and 0.37 for TP (P = 0.014). Regarding + sIgE, this SPT criterion has a specificity of 92.2% for DP, 82.3% for DF, 80.8% for LD and 70.1% for TP. When the value 4.5 mm was taken as the threshold for the positive SPT reaction to TP, specificity increased significantly from 70.1% to 86.4% (χ2 = 32.04, P < 0.001).Conclusions: The 3 mm SPT threshold criterion is not reliable in evaluating sensitization to TP due to an insufficient specificity of the allergen extract to this mite. It is advisable either to re‐evaluate the TP allergen extract or change the threshold criterion for positive SPT reaction to TP.
The increased histamine release in ischaemic heart disease patients undergoing coronaroangiography is not mediated by specific IgE
Authors: Rodriguez R. M. , Guéant J.‐L. , Aimone‐Gastin I. , Gérard P. , Amoghly F. , Bellou A. , Jullière Y. , Faure G. , Danchin N. , Romano A. 
Published: March 2003

Background:  The release of histamine by iodinated contrast media (ICM) is higher in coronary artery disease patients than in noncoronary patients during coronary angiogram. Methods:  Eighty‐eight patients who underwent a coronary angiography were classified either as having coronary artery disease or as noncoronary patients. Histamine concentration was higher than the 6.8 nM upper limit in 7 cases (group 1), of whom six were coronary artery disease patients. We compared the IgE and complement fractions in plasma of these patients to two control groups with normal histamine blood level, one (group 2) with and the other (group 3) without coronary artery disease. Results:
No difference of total IgE and C
3c
and C
4 complement fractions was found among the three groups. Anti‐ioxaglate IgE‐RIA was positive in only one patient from group 1. The affinity of drug‐IgE binding in the serum of this patient was very low (Kd: 18.7 mM). The level of anti‐ICM IgE detected by ioxitalamate‐ and iomeprol‐Sepharose RIA was significantly higher in groups 2 and 3 than in group 1. Conclusions:  The higher histamine release in ischaemic heart disease patients undergoing coronaroangiography is not mediated by IgE or complement activation. Further studies are needed to investigate the implication of histamine release factors.
Tuberculin responses in children with allergic diseases
Authors: Ozmen S. , Tomac N. , Uysal A. , Arslan Z. , Kuyucu N. , Yoney A. 
Published: March 2003

Background: The prevalence of allergic disorders has been increasing over the last 30 years, especially in developed countries. One factor associated with this rise may be the decline of many childhood infections. We investigated tuberculin responses in allergic children in order to see the development of delayed‐type hypersensitivity reactions to tubercule bacillus infection.Methods: The study sample was composed of 106 allergic and 100 nonallergic children vaccinated with bacille Calmette‐Guerin (BCG). The standard Mantoux test was applied to all children. The reactions were read after 72 h by measuring the diameter of the wheal.Results: The wheal size was 6.29 ± 5.09 mm (mean ± SD) in allergic children, and 2.79 ± 2.96 mm in nonallergic children. The difference between the two groups was significant (P < 0.001). In children with a single BCG scar, the mean purified protein derivative (PPD) wheal size for allergic children was 4.77 ± 4.79 mm, and for nonallergic children it was 2.48 ± 3.19 mm. The mean PPD wheal sizes in allergic and nonallergic children who had been vaccinated twice were 8.35 ± 4.80 mm and 3.33 ± 2.44 mm, respectively. This difference was statistically significant (P < 0.05). In 27.35% of the allergic children and 6% of the nonallergic children, the positive tuberculin responses (PPD ≥ 10 mm) were recorded. The difference was significant (P < 0.05).Conclusions: Our results showed that response to tuberculin in BCG‐immunized allergic children is higher than in BCG‐immunized nonallergic children.
Tetraploidy in a 26‐month‐old girl (cytogenetic and molecular studies)
Authors: Guc‐Scekic M , Milasin J , Stevanovic M , Stojanov LJ , Djordjevic M 
Published: March 2003

Liveborn infants with tetraploidy are very rare in human pregnancies and usually die during the first days or months. Seven cases of liveborn infants with tetraploidy have previously been reported. Among them only two 92, XXXX infants survived for longer than 12 months. Here we report on the case of a 26‐month‐old girl with tetraploidy. The main clinical features of tetraploidy are facial dysmorphism, severely delayed growth and developmental delay.On the basis of molecular studies we discuss the possible origin of the additional chromosome sets in our proband. To our knowledge, this infant is the first reported case of tetraploidy who lived up to 26 months.
Mycophenolic acid concentrations in long‐term heart transplant patients: relationship with calcineurin antagonists and acute rejection
Authors: Cantin Bernard , Giannetti Nadia , Parekh Hemal , Panchal Shyam N , Kwok Bernard W‐K , Najem Ruhina , Woodman Katina , Hunt Sharon A , Valantine Hannah A 
Published: March 2003

Background: When used in conjunction with steroids and cyclosporin, mycophenolate mofetil (MMF) has been shown to significantly reduce mortality and incidence of rejection in the first year after heart transplantation. It also appears that in this early post‐transplantation period, the monitoring of immunosuppressive therapies may be warranted. The current study was undertaken to determine if such monitoring is still useful more than 1 yr after heart transplantation.Methods: Twenty‐six patients who had survived the first year after orthotopic heart transplantation and had been on MMF therapy for more than 3 months were prospectively followed. At the time of their routine endomyocardial biopsy blood samples were taken to monitor immunosuppressive therapy. Most patients had two samples taken, on average 109 d apart.Results: There were 22 episodes of asymptomatic rejection documented on a total of 48 biopsies. Of these, only two were of ISHLT (International Society for Heart and Lung Transplantation) grade 3A the remainder being of ISHLT grades 1 or 2. There was no relation between immunosuppressive regimen (tacrolimus and MMF or cyclosporin and MMF) and rejection. There was no relation between monitored immunosuppressive levels and rejection. Patients with the combination of MMF and tacrolimus had significantly higher plasma mycophenolic acid levels despite significantly lower daily MMF dose.Conclusion: There does not appear to be a benefit in continued monitoring of plasma mycophenolic acid levels beyond the first year of heart transplantation. There were significant differences in plasma mycophenolic acid levels depending on the type of calcineurin inhibitor concomitantly used.
A measure for assessing patient perception of provider support for self‐management of bipolar disorder
Authors: Ludman Evette J , Simon Gregory E , Rutter Carolyn M , Bauer Mark S , Unützer Jürgen 
Published: March 2003

Objectives:
Health care providers have an important role in acknowledging and supporting patients' self‐management of chronic illnesses such as bipolar disorder. This report describes the development and evaluation of a brief measure for assessing patient perception of providers' support for self‐management of bipolar disorder.
Methods:
A 10‐item measure was developed combining generic items from an existing measure of providers' autonomy supportive versus controlling style with items specific to the self‐management of bipolar disorder. The psychometric properties of the measure and its relation to clinical variables were evaluated in a sample of patients enrolled in an ongoing randomized intervention trial.
Results:
Data were obtained from 420 patients with a chart diagnosis of bipolar disorder (mean age=44, 68% female, 88% Caucasian). The proportion of missing responses for items ranged from 0 to 3%. Reliability coefficient alpha for the full scale was 0.94. Corrected item–total correlations for individual items ranged from 0.70 to 0.83. Factor analysis identified a single factor accounting for 67% of total variance. Factor loadings for individual items were all at least 0.75. The measure showed moderate positive correlations with measures of self‐efficacy for managing bipolar disorder (r=0.34; p < 0.001), treatment satisfaction (r=0.63; p < 0.001), small negative correlations with measures of mania symptoms (r=–0.11; p < 0.03) and depressive symptoms (r=–0.09; p < 0.10).
Conclusions:
This measure shows good psychometric properties and good evidence for convergent and discriminant validity. It is promising for assessing an important aspect of care for bipolar disorder.
Infection, treatment and immune response in patients with bipolar disorder versus patients with major depression, schizophrenia or healthy controls
Authors: Hinze‐Selch Dunja 
Published: March 2003

Hinze‐Selch D. Infection, treatment and immune response in patients with bipolar disorder versus patients with major depression, schizophrenia or healthy controls.  Bipolar Disord 2002: 4(Suppl. 1): 81–83. ©Blackwell Munksgaard, 2002Bipolar disorder is the least studied among the three major psychiatric disorders of schizophrenia, major depression and bipolar disorder. Furthermore, investigations on infection and immunity in bipolar disorder make up only a small portion of the sparse research done on this disorder. However, there are reports that modulation of the immune system and certain infections might be associated with bipolar disorder and that there might be differences between bipolar and the other disorders. The purpose of this paper is to briefly review published data on these issues in bipolar versus the other disorders, and to present an ongoing clinical study on the putative involvement of infection with the parasite Toxoplasma gondii in these three major psychiatric disorders.
Etiology and outcome of second trimester non‐immunologic fetal hydrops
Authors: HEINONEN SEPPO , RYYNÄNEN MARKKU , KIRKINEN PERTTI 
Published: March 2003

Background. This investigation was undertaken to study the conditions resulting in midtrimester fetal hydrops and to evaluate its overall prognosis as regards counseling purposes.Material and methods. The etiology of midtrimester non‐immune fetal hydrops was retrospectively evaluated as regards cases detected in singleton pregnancies (n=58) from January 1987 through December 1996. The condition was diagnosed by way of routine biochemical and ultrasonographic screening of a pregnant population.Results. The rate of diagnosed midtrimester fetal hydrops was 1 in 1700 pregnancies and the overall survival rate in this usually unexpected condition was less than 10%. Various chromosomal and fetal structural abnormalities dominated as a cause of fetal hydrops, representing 44.8% and 43.1% of the cases, respectively. Infection caused only 6.9%. The underlying etiology remained unknown in only three cases. However, in 78% the diagnosis was already known prenatally.Discussion. Although the overall prognosis is poor, knowledge of the primary etiology is important to determine a rational therapeutic strategy. If the results of routine investigations rule out malformation and abnormal chromosomes, follow‐up serial ultrasonographic assessment may indicate that the hydropic state is transient, carrying a somewhat better prognosis.