bluuebunny/crossref_metadata_2025_split huggingface dataset:
High biological N fixation potential dominated by heterotrophic diazotrophs in alpine permafrost rivers on the Qinghai-Tibet Plateau
Authors: Xin Yuan , Gao Qun , Chen Xin , Sun Siyue , Liu Jiao , Gao Hui , Zhou Jizhong , Xia Xinghui 
Published: February 2024

Abstract
Background
Biological nitrogen (N) fixation is a pivotal N source in N-deficient ecosystems. The alpine permafrost rivers on the Qinghai‒Tibet Plateau (QTP), which are recognized as N-limited and sub-anoxic environments, provide a suitable habitat for diazotrophs. However, the diversity, structure, and ecological functions of diazotrophic communities in these alpine permafrost QTP rivers remain poorly understood. Here, we examined diazotrophic communities in QTP rivers via the nitrogenase (nifH) gene sequencing and assessed their N-fixing activities via a 15N isotope incubation assay. We also investigated the regulating mechanism of altitude and other geochemical factors on the diazotrophic communities and their associated N-fixing activities in QTP rivers.
Results
Strikingly, anaerobic heterotrophic diazotrophs, such as sulfate- and iron-reducing bacteria, had emerged as dominant N fixers, overshadowing the contributions of well-known phototrophic cyanobacterial diazotrophs. Remarkably, the nifH gene abundance and N fixation rates increased with altitude, and the average nifH gene abundance (2.57 ± 2.60 × 108 copies g− 1) and N fixation rate (2.29 ± 3.36 nmol N g− 1 d− 1) surpassed that documented in most aquatic environments. Such distinctive heterotrophic diazotrophic communities and high N fixation potential in QTP rivers were associated with low-nitrogen, abundant organic carbon and special C:N:P stoichiometries. Additionally, the significant presence of psychrophilic bacteria within the diazotrophic communities, along with the enhanced stability and complexity of the diazotrophic networks at higher altitudes, clearly demonstrate the adaptability of diazotrophic communities to extreme cold and high-altitude conditions in QTP rivers. We further determined that altitude, coupled with organic carbon and C:N:P stoichiometries, was the predominant driver shaping diazotrophic communities and their N-fixing activities. Based on the N fixation rates obtained in this study, the biological N fixation was estimated to be 2.79 ± 4.10 Gg N yr− 1 in QTP rivers.
Conclusions
The alpine permafrost rivers on the Qinghai-Tibet Plateau (QTP) possess distinctive heterotrophic diazotrophic communities and high biological N fixation potential, which are associated with altitude, abundant organic carbon, and special C:N:P stoichiometries. The estimated nitrogen input into the QTP rivers by biological N fixation would largely compensate for the nitrogen loss by denitrification. Overall, our study provides novel insights into nitrogen dynamics in alpine permafrost rivers.
Understanding distress in patients before breast cancer diagnosis in China：A Mixed Methods Study
Authors: Gao Yan , Wang Linying , Guo Jun , Gao Jinnan 
Published: February 2024

Abstract
Purpose: Although there is extensive research on the link between breast cancer diagnosis and psychological distress after diagnosis, there is limited data on the prevalence of psychological distress prior to breast cancer diagnosis. The purpose of this study is to measure the distress levels of patients before breast cancer diagnosis, to identify the categories of distress they have, and to describe the experience of distress of patients before diagnosis.
Methods: This study uses a mixed method of survey and qualitative interviews. In the quantitative part, a cross-sectional survey of 118 patients with completed breast biopsies awaiting pathological diagnosis was conducted using the National Comprehensive Cancer Network's (NCCN's) Distress Thermometer(DT) and Problem List (PL), In the qualitative part, 15 patients were invited using convenient and purposive sampling. Data was collected using a semi-structured face-to-face interview and recorded. The transcribed text was analyzed using Colaizzi's phenomenological method.
Results: The results reveal that 63.6% of patients reported a higher level of psychological distress (n=75, scoring≥4).Most patients had emotional (98%) or physical (64%) problems. Among these, worry or anxiety (47.4%), fear (33%), finances (21.1%), sadness and depression(18.6%), and grief or loss(15.2%) were the most frequently reported problems. Multivariate logistic regression analysis showed that the factors associated with psychological distress before the diagnosis of breast cancer patients were age, education level and employment. The experience of patients before diagnosis emerged four themes: Very high psychological distress index; Sources of psychological distress; The demand for information; Positive coping.
Conclusions: Psychological distress is prevalent prior to breast cancer diagnosis and may merit early intervention. Gaining insight into patients' emotional distress can assist in shaping future evaluations and interventions focused on their psychosocial needs, to enhance their overall well-being.
Impact of mechanical power on mortality in ventilated critically ill patients. Retrospective study with continuous real-life data
Authors: Manrique Sara , Ruiz-Botella Manuel , Murillo Natalia , Canelles Sandra , Victoria Ivan David , Samper Manuel Andres , Plans Oriol , Claverias Laura , Magret Monica , Gordo Federico , Roca Oriol , Bodi Maria 
Published: February 2024

Abstract
Background
Over the past decade, numerous studies on potential factors contributing to ventilation-induced lung injury have been carried out. Mechanical power has been pointed out as the parameter that encloses all ventilation-induced lung injury-contributing factors. However, studies conducted to date provide data regarding mechanical power during the early hours of mechanical ventilation that may not correspond to the real scenario.
Methods
Retrospective observational study conducted at a single center in Spain. Patients admitted to the intensive care unit, > o = 18 years of age, and ventilated for over 24 hours were included.
We extracted the mechanical power values throughtout the entire mechanical ventilation period from the clinical information system every two minutes. First, we calculate the cutoff-point for mechanical power beyond which there was a greater change in the probability of death. After, the sum of time values above the safe cut-off point was calculated to obtain the value in hours. We analyzed if the number of hours the patient was under ventilation with a mechanical power above the safe threshold was associated with mortality, invasive mechanical ventilation days, and intensive care unit length of stay. We repeated the analysis in different subgroups based on the degree of hypoxemia and in patients with SARS CoV-2 pneumonia.
Results
The cut-off point of mechanical power at with there is a higher increase in mortality was 18J/min. The greater the number or hours patients were under mechanical power > 18 J/min the higher the mortality in all the study population, in patients with SARS CoV-2 pneumonia and in mild to moderate hyopoxemic respiratory failure. The risk of death inceases 0.1% for each our with mechanical power exceeding 18 J/min. The number of hours with mechanical power > 18 J/min also affected the days of invasive mechanical ventilation and intensive care unit length of stay.
Conclusions
Continuous monitoring of mechanical power using an automated clinical information system shows that the number of hours with mechanical power > 18 J/min increases mortality in critically ill patients.
Evaluation of multidisciplinary high-risk pregnancy clinic for myelomeningocele
Authors: Anderson Luke , Hopson Betsy , Caudill Caroline , Rocque Brandon G. , Blount Jeffrey , Arynchyna-Smith Anastasia , Thrower Jessica , Johnston James , Rozzelle Curtis 
Published: February 2024

Abstract

A cross-sectional study retrospectively evaluating the perceived usefulness of attending a multi-disciplinary, roundtable, educational prenatal clinic for mothers expecting children with myelomeningocele is presented. Mothers who currently have children with SB completed a survey which evaluated their overall preparedness, spina bifida education, delivery plans, surgical expectations, and expectations in terms of quality of life and development. Open comments were also collected. Approximately half of these mothers received some form of prenatal SB counseling. Statistical analysis was performed to identify differences between those who attended prenatal counseling and those who did not. Mothers who attended prenatal counseling reported that they felt more informed and prepared throughout their pregnancy, during the delivery of their child, and during their initial hospital stay than mothers who did not. They reported that the roundtable discussions were beneficial, and the education they received was useful in helping them form accurate expectations and feel more at ease. This suggests that prenatal counseling and the High-Risk Pregnancy Clinic (HRPC) provides perceived utility to families and mothers and that the HRPC is an effective method of providing prenatal counseling to mothers whose unborn children have been diagnosed with myelomeningocele.
Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression
Authors: Li Lixia , Liu Dewei , Chen Tingting , Wei Chunhui , Qiao Youping , Liu Weiliang , Liang Yanmei , Liang Zhu , Chen Chunyuan , Li Dongming , Wu Bin , Zhao Xuanna , Huang Dan , Wu Dong 
Published: February 2024

Abstract

Background
The progression of non-small cell lung cancer (NSCLC) is significantly influenced by circular RNAs (circRNAs), especially in tumor hypoxia microenvironment. However, the precise functions and underlying mechanisms of dysregulated circRNAs in NSCLC remain largely unexplored.
Methods
Differentially expressed circRNAs in NSCLC tissues were identified through high-throughput RNA sequencing. The characteristics of circ_0007386 were rigorously confirmed via Sanger sequencing, RNase R treatment and actinomycin D treatment. The effects of circ_0007386 on proliferation and apoptosis were investigated using CCK8, cloning formation assays, TUNEL staining, and flow cytometry assays in vitro. In vivo, xenograft tumor models were used to evaluate its impact on proliferation. Mechanistically, the regulatory relationships of circ_0007386, miR-383-5p and CIRBP were examined through dual luciferase reporter assays and rescue experiments. Additionally, we detected the binding of EIF4A3 to CRIM1 pre-mRNA by RNA immunoprecipitation and the interaction between YAP1 and EIF4A3 under hypoxic conditions by co-immunoprecipitation.
Results
Our investigation revealed a novel circRNA, designated as circ_0007386, that was upregulated in NSCLC tissues and cell lines. Circ_0007386 modulated proliferation and apoptosis in NSCLC both in vitro and in vivo. Functionally, circ_0007386 acted as a sponge for miR-383-5p, targeting CIRBP, which influenced NSCLC cell proliferation and apoptosis via the PI3K/AKT signaling pathway. Furthermore, under hypoxic conditions, the interaction between YAP1 and EIF4A3 was enhanced, leading to the displacement of EIF4A4 from binding to CRIM1 pre-mRNA. This facilitated the back-splicing of CRIM1 pre-mRNA, increasing the formation of circ_0007386. The circ_0007386/miR-383-5p/CIRBP axis was significantly associated with the clinical features and prognosis of NSCLC patients.
Conclusions
Circ_0007386, regulated by YAP1-EIF4A3 interaction under hypoxia conditions, plays an oncogenic role in NSCLC progression via the miR-383-5p/CIRBP axis.
Local Delivery of Accutox® Synergises With Immune-checkpoint Inhibitors at Disrupting Tumor Growth
Authors: Bikoriman Jean-Pierre , Hachme Nehme , Abusara Jamilah , Gonçalves Marina Pereira , Farah Roudy , Talbot Sebastien , Beaudoin Simon , Stanga Daniela , Plouffe Sebastien , Rafei Moutih 
Published: February 2024

Abstract

Background: The Accum® platform was initially designed to accumulate biomedicines in target cells by inducing endosomal-to-cytosol escape. Interestingly however, the use of unconjugated Accum® was observed to trigger cell death in a variety of cancer cell lines; a property that was further exploited in the development of Accum®-based anti-cancer therapies. Despite the impressive pro-killing abilities of the parental molecule, some cancer cell lines exhibited resistance. This prompt us to test additional Accum® variants, which led to the identification of the AccuTOX® molecule.
Methods: A series of flow-cytometry and cell-based assays were used to assess the pro-killing properties of AccuTOX® along with its ability to trigger the production of reactive oxygen species (ROS), endosomal breaks and antigen presentation. RNA-seq was also conducted to pinpoint the most prominent processes modulated by AccuTOX® treatment in EL4 T-cell lymphoma. Finally, the therapeutic potency of intratumorally-injected AccuTOX® was evaluated in three different solid tumors models (EL4, E0771 and B16) both as a monotherapy or in combination with three immune-checkpoint inhibitors (ICI).
Results: In total, 7 Accum® variants were screened for their ability to induce complete cell death in 3 murine (EL4, B16 and E0771) and 3 human (MBA-MD-468, A549, and H460) cancer cell lines of different origins. The selected compound (hereafter refereed to as AccuTOX®) not only displayed an improved killing efficiency (~5.5 fold compared to the parental Accum®), but it did so while retaining its ability to trigger immunogenic cell death, ROS production, and endosomal breaks. Moreover, transcriptomic analysis revealed that low dose AccuTOX® enhances H2-Kb cell surface expression as well as antigen presentation in cancer cells. The net outcome culminates in impaired T-cell lymphoma, breast cancer and melanoma growth in vivo especially when combined with anti-CD47, anti-CTLA-4 or anti-PD-1 depending on the animal model.
Conclusions: AccuTOX® exhibits enhanced cancer killing properties, retains all the innate characteristics displayed by the parental Accum® molecule, and synergizes with various ICI in controlling tumor growth. These observations will certainly pave the path to continue the clinical development of this lead compound against multiple solid tumor indications.
Factors influencing the physio psychosocial state and health concerns of Canadian and Iranian undergraduate nursing students during the pandemic
Authors: Okuyan Canan Birimoglu , Begen Mehmet , Mohtashami Jamileh , Güngörmüş Zeynep 
Published: February 2024

Abstract
The aim of our study is to investigate and analyze the factors that influence the physical, psychological, and social well-being of nursing students in Canada and Iran amidst the COVID-19 pandemic.The current study is both descriptive and comparative. Nursing students from both Canadian and Iranian institutions. Data was collected using a questionnaire based on the Short Health Anxiety Inventory (SHAI) scale and the Physio-Psychosocial Response Scale (PPSRS).
There were statistically significant positive and extremely strong correlations (respectively; r=.911, r=.964, r=.952). between the total score of the physio-psycho-social response scale and the social, emotional, and somatic subscales of the students' subgroups. According to our findings, the COVID-19 pandemic has had a negative impact on the physio-psycho-social well-being of Canadian and Iranian nursing students. The total scores of the physio psychosocial response scale and the health anxiety scale were found to have a statistically significant positive relationship.The research findings support that during the pandemic, psychological support initiatives for nursing students should be prioritized. Moreover, the present study could be valuable in assessing the immediate psychological needs of the general population who are encountering physical symptoms during the epidemic.
Efficient Server Room Monitoring with Flutterand Firebase - Real-Time Insights and InstantNotifications
Authors: Özkurt Cem 
Published: February 2024

Abstract

A comprehensive server room monitoring system is presented in this research,leveraging Raspberry Pi devices, DHT11 temperature/humidity sensors, PIRmotion sensors, and magnetic reed switches. Integration of Flutter and Firebasetechnologies enabled real-time environmental monitoring, security event detection, and remote access control. The effectiveness of Raspberry Pi devices infacilitating cost-effective and scalable monitoring solutions was demonstrated,while Flutter and Firebase enhanced accessibility, usability, and responsiveness.The aim of this research is to improve server room management, security, andoperational efficiency, contributing to a more secure and resilient infrastructurein the digital age
Global research trends on sepsis and septic shock during 2003–2022: A data-driven bibliometric analysis
Authors: Han Weiying , Guo Haoxin , Wang Zhongqing , Ding Renyu 
Published: February 2024

Abstract
Background
Sepsis is a major medical condition that contributes to substantial morbidity and mortality rates worldwide. Research to better understand and manage these complex conditions has intensified over the past two decades. This bibliometric analysis aims to map global research trends in sepsis and septic shock from 2003 to 2022, providing insights into the evolution of the field.
Methods
This study conducted a comprehensive search for publications on sepsis and septic shock using the Web of Science Core Collection. Data were analysed using VOSviewer for bibliometric mapping, focusing on publication trends, country, institutional contributions, journal sources, citation analysis, and keyword co-occurrence.
Results
In total, 78,108 publications were retrieved, with a notable increase in annual output over the study period. The United States ranked first in publication volume, followed by China, Germany, and the United Kingdom. Harvard University was the most prolific institution. Critical Care Medicine emerged as the leading journal in terms of publication count. The most cited articles and references were predominantly related to definition, epidemiology, and management guidelines for sepsis. Recent research hotspots included COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA).
Conclusions
Our bibliometric analysis identifies global research trends in sepsis over the past 20 years, with evolving hotspots and increasing global collaboration. Key findings highlight continuous efforts to understand the complex pathophysiology of sepsis and septic shock. COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA) are hotspots.
Diffusely adherent and cyclomodulin- positive Escherichia coli are associated with colon diseases
Authors: Hosseininasab Seyed_Abolfazl , Saffari Fereshteh , Tadjrobehkar Omid , Zandi Hengameh , Ahmadi Bijan , Khoshnood Asghar , Niksefat Naser , Mehboodi Mahtab 
Published: February 2024

Abstract
Any alteration in gut microbiome may result in colonization of some pathobionts and consequently some colon diseases. The present study was performed to determine association between E.coli pathobionts (cyclomodulin positive and afa-C + diffusely adherent E.coli) and also some characteristics of them in patients with colon diseases. Stool specimens were obtained from patients referred to colonoscopy centers of university hospitals of Yazd and Kerman, Iran. Totally 67 patients voluntarily joined the study as target group (21 case of colorectal cancer and 46 case of inflammatory bowel disease) as well as 67 healthy individuals. Stool samples were screened for Escherichia coli isolates by culture technique. Cyclomodulin encoding genes (clbN, cnf, cdt and cif) as well as afa-C, were tracked by PCR assay. Phylogrouping, virulence gene screening, antibiotic susceptibility evaluation and biofilm formation assessment were also performed. Results showed clbN, cnf and afa-C + DAEC were significantly associated with CRC. The latter was also associated with IBD (p < 0.05). Most of the isolates from patients group, belonged to B2 phylogroup. Iron uptake related genes were significantly associated with patients. Antibiotic resistance and biofilm formation were not significantly associated with any studied groups. In conclusion, this study provides primarily data about the status of some important E.coli pathobionts involved in colon diseases. It appears that afa-C + DAEC was more associated with colon diseases so it could be proposed as a putative marker for screening procedures. However, as few of specimens were positive for afa-C and cyclomodulins (clb and cnf), definitive conclusion require more comprehensive investigation.
Provider and community perspectives of integrated COVID-19 and routine childhood immunisation programmes in Nigeria: a qualitative exploratory study
Authors: Bakare Ayobami A. , Akinsola Kofoworola O. , King Carina , Sogbesan Abiodun A. , Bakare Oluwabunmi R. , Fadahunsi Opeyemi Y. , Salako Julius , Falade Adegoke G. , Wees Sibylle Herzig 
Published: February 2024

Abstract

Background
In Nigeria, COVID-19 vaccines were delivered through outreach activities and integrated with routine immunisation. However, evaluations of integrated approaches for novel vaccines are scarce. We aimed to understand the perceived benefits and challenges of integrating the COVID-19 vaccine within routine- immunisation in Nigeria and identify ways to strengthen this approach.
Methods
We conducted 30 semi-structured interviews with community members and healthcare workers in primary healthcare (PHC) facilities in Jigawa (n = 16) and Oyo (n = 14) states, Nigeria in August 2022. Participants were selected purposively from PHC facilities. Healthcare worker and community interviews were analysed separately using thematic analysis.
Results
We identified four themes that describe the community response, perceived impact, and health system adaptions to the challenges associated with the integrated vaccine delivery approach. Community members expressed concern that children might be given COVID-19 vaccines instead of routine immunisations, while others appreciated the integrated approach due to their trust in the efficacy of COVID-19 vaccines, government, and healthcare providers. Healthcare providers perceived the integrated approach as improving vaccination coverage and awareness but noted additional problems of increased workload, vaccine scarcity, and prolonged clinic visits. Insufficient resources were subsisting barriers to effective integration in both states, but the provider’s gender was also a challenge in Jigawa state. Additionally, the use of incentives to generate demand had ambiguous effects in Jigawa state.
Conclusion
Taking an integrated approach to deliver COVID-19 vaccines was acceptable by health workers but resisted from the community. Addressing persistent challenges in existing vaccination programmes is pertinent to enhance effectiveness and acceptability of the integrated approach.