bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sistem Pendukung Keputusan Dalam Pemilihan Supplier Kopi Menerapkan Metode OCRA Dengan Pembobotan ROC
Authors: Chandra Novyanti , Trista Ayunda Afifah 
Published: October 2023

Perkembangan bisnis kopi terus berkembang pesat sehingga membuat pelaku bisnis kopi untuk mencari supplier kopi yang berkualitas dan dapat memenuhi kebutuhan para konsumennya. Dalam pemilihan supplier kopi, penting untuk dilakukan dengan baik karena dapat mempertahankan keberadaan para pelanggannya. Namun, seringkali dalam pemilihan supplier kopi ditemukan sebuah masalah dalam proses seleksinya, seperti dalam mengelola informasi yang kompleks, dan melakukan evaluasi, serta pemilihan calon supplier tidak dilakukan secara tersistem. Maka dari itu, diperlukan sistem pendukung keputusan dalam memberikan sebuah keputusan yang akurat dan cepat dengan menggunakan metode OCRA dan proses pembobotan yang dihasilkan dari metode ROC. Penentuan supplier kopi ini, memiliki 5 kriteria, yaitu harga, kualitas kopi, variasi roasted level, aroma, dan lokasi. Hasil dari penilaian menggunakan metode ROC dan metode OCRA menghasilkan alternatif terbaik sebagai supplier kopi adalah bernama The Cold Crafters dengan nilai preferensi 1.2453
Implantação do Plano Terapêutico Singular na Atenção Básica: Fortalezas e possíveis obstáculos
Authors: Guimarães Ana Clara Ribeiro , Pereira Queli Lisiane Castro , Ferreira Adriano Borges 
Published: October 2023

O presente artigo busca relatar fatores potencializadores e dificultadores à implementação do Plano Terapêutico Singular na Atenção Básica na percepção dos profissionais de saúde. É exploratório, descritivo e transversal com abordagem qualitativa. Realizado com 15 preceptores/ profissionais de saúde da Atenção Básica de Pontal do Araguaia (MT). Da análise temática emergiram as categorias: fortalezas para implementação do PTS e possíveis obstáculos. Frente ao processo de trabalho em saúde em seus territórios, os profissionais dos serviços de saúde identificaram resolutividade, vínculo, integralidade, prática interprofissional colaborativa e a centralidade no usuário como dispositivos motivadores à implementação. Enquanto a falta de pertencimento /comprometimento, a escassez de planejamento e sobrecarga de trabalho poderão ser obstáculos, na percepção dos profissionais. Em síntese, os resultados obtidos servirão como base para nortear a implementação do PTS na AB do município e em outras Secretarias de Saúde visto que se identificou elementos facilitadores a sua efetivação assim como os obstáculos.
Technical report: liquid overlay technique allows the generation of homogeneous osteosarcoma, glioblastoma, lung and prostate adenocarcinoma spheroids that can be used for drug cytotoxicity measurements
Authors: Jubelin Camille , Muñoz-Garcia Javier , Cochonneau Denis , Ollivier Emilie , Vallette François , Heymann Marie-Françoise , Oliver Lisa , Heymann Dominique 
Published: October 2023

Introduction: The mechanisms involved in cancer initiation, progression, drug resistance, and disease recurrence are traditionally investigated through in vitro adherent monolayer (2D) cell models. However, solid malignant tumor growth is characterized by progression in three dimensions (3D), and an increasing amount of evidence suggests that 3D culture models, such as spheroids, are suitable for mimicking cancer development. The aim of this report was to reaffirm the relevance of simpler 3D culture methods to produce highly reproducible spheroids, especially in the context of drug cytotoxicity measurements.Methods: Human A549 lung adenocarcinoma, LnCaP prostate adenocarcinoma, MNNG/HOS osteosarcoma and U251 glioblastoma cell lines were grown into spheroids for 20 days using either Liquid Overlay Technique (LOT) or Hanging Drop (HD) in various culture plates. Their morphology was examined by microscopy. Sensitivity to doxorubicin was compared between MNNG/HOS cells grown in 2D and 3D.Results: For all cell lines studied, the morphology of spheroids generated in round-bottom multiwell plates was more repeatable than that of those generated in flat-bottom multiwell plates. HD had no significant advantage over LOT when the spheroids were cultured in round-bottom plates. Finally, the IC50 of doxorubicin on MNNG/HOS cultured in 3D was 18.8 times higher than in 2D cultures (3D IC50 = 15.07 ± 0.3 µM; 2D IC50 = 0.8 ± 0.4 µM; *p &lt; 0.05).Discussion: In conclusion, we propose that the LOT method, despite and because of its simplicity, is a relevant 3D model for drug response measurements that could be scaled up for high throughput screening.
Echocardiography and laboratory outcomes of COVID-19 in children with a history of Kawasaki disease: a preliminary observation
Authors: Guo Mindy Ming-Huey , Chang Ling-Sai , Chen Yu-Jhen , Kuo Ho-Chang 
Published: October 2023

BackgroundInfection with SARS-CoV-2 virus has been associated with cardiovascular sequelae including multisystem inflammatory syndrome (MIS-C) in children. Patients with a prior history of Kawasaki disease, may be more susceptible to changes in echocardiographic or laboratory findings after COVID-19. The objective of this study was to investigate the echocardiographic and laboratory findings in children with a prior history of Kawasaki disease after SARS-CoV-2 infection.Materials and methodsIn this study, we performed a retrospective chart review of 41 children younger than 18 years old who were diagnosed with COVID-19 from April to August of 2022 and had a prior history KD. We included echocardiography and blood draw data obtained at the last outpatient follow-up at our hospital for KD, and within 4 months of SARS-CoV-2 infection. Echocardiographic data obtained from 82 age-matched and gender matched controls were also included for comparison.ResultsWe found that COVID-19 resulted in slightly higher RCA Z-scores within the first month after infection (mean ± SE, 1.20 ± 0.18 vs. 0.83 ± 0.18, p = 0.030), although this increase did not result in coronary artery dilatation, defined as a Z-score of at least 2.5. In addition, we found that degree of RCA dilatation after COVID-19 infection was negatively correlated with the change in monocyte percentage (Pearson's correlation coefficient—0.363, p = 0.020). Moreover, RCA Z-score changes were lower in patients who received at least one dose of mRNA COVID-19 vaccine when compared those who did not receive any (mean ± SE, −0.23 ± 0.16 vs. 0.39 ± 0.17, p = 0.031).ConclusionIn this pilot study we found that COVID-19 infection resulted in slightly higher RCA Z-scores in children with a prior history of KD, although not large enough to be classified as coronary aneurysms. While these changes could be the result of measurement imprecision or interobserver variation, further study of the cardiac outcomes of COVID-19 infection in children with a prior history of KD are needed in the future.
Abductive reasoning in modeling biological phenomena as complex systems
Authors: Engelschalt Paul , Röske Maxime , Penzlin Johanna , Krüger Dirk , Upmeier zu Belzen Annette 
Published: October 2023

IntroductionAbductive reasoning is a type of reasoning that is applied to generate causal explanations. Modeling for inquiry is an important practice in science and science education that involves constructing models as causal explanations for scientific phenomena. Thus, abductive reasoning is applied in modeling for inquiry. Biological phenomena are often best explained as complex systems, which means that their explanations ideally include causes and mechanisms on different organizational levels. In this study, we investigate the role of abductive reasoning in modeling for inquiry and its potential for explaining biological phenomena as complex systems.MethodsEighteen pre-service science teachers were randomly assigned to model one of two biological phenomena: either a person's reddened face, for which participants knew of explanations from their everyday lives, or a clownfish changing its sex, for which participants did not know about explanations. Using the think-aloud method, we examined the presence of abductive reasoning in participants' modeling processes. We also analyzed modeling processes in terms of participants' ability to model the phenomena as complex systems.ResultsAll participants reasoned abductively when solving the modeling task. However, modeling processes differed depending on the phenomenon. For the reddened face, participants generated simple models that they were confident with. In contrast, for the clownfish, participants generated more complex models that they were insecure about. Extensive engagement in abductive reasoning alone did not lead to the generation of models that explained the phenomena as complex systems.DiscussionBased on the findings, we conclude that engagement in abductive reasoning will not suffice to explain phenomena as complex systems. We suggest examining in future studies how abductive reasoning is combined with systems thinking skills to explain phenomena as complex systems in biological model construction.
Multiple endocrine neoplasia type 1 with Zollinger–Ellison syndrome: clinicopathological analysis of a Japanese family with focus on menin immunohistochemistry
Authors: Kimura Noriko , Hirata Yasuji , Iwashiro Nozomu , Kijima Hiroshi , Takayasu Shinobu , Yamagata Satoshi , Sakihara Satoru , Uchino Shinya , Ohara Masanori 
Published: October 2023

BackgroundMultiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder characterized by the occurrence of multiple epithelial neuroendocrine tumors (NETs) and non-NETs in various organs. MEN1 encodes a 610-amino acid-long tumor suppressor protein, menin. The optimal treatment for multiple tumors, identification of the most critical tumors for patient prognosis, and menin immunohistochemistry findings remain controversial. Therefore, we aimed to elucidate these issues through a histological analysis of tumors and tumor-like lesions in a Japanese family, comprising a father and his two sons, who had MEN1 with Zollinger–Ellison syndrome (ZES).Patients and methodsAll family members had a germline alteration in exon 10, c.1714-1715 del TC of MEN1, and exhibited multiple synchronous and metachronous tumors. The patients had pulmonary NETs, hyperparathyroidism, hypergastrinemia, pituitary adenomas, pancreaticoduodenal NETs, adrenocortical adenoma with myelolipoma, nodular goiter of the thyroid, lipomas, and angiofibroma. Most tumors were resected and histologically examined. We compared their clinical courses and tumor histology, and conducted menin immunohistochemistry (IHC).ResultsTwo patients died of pulmonary NET G2. One patient who underwent pancreaticoduodenectomy was cured of ZES; however, the two other patients who did not undergo pancreaticoduodenectomy suffered persistent ZES despite treatment with octreotide. Menin IHC revealed varying NET intensities, ranging from positive to negative stains.ConclusionPancreaticoduodenectomy is the most effective treatment for ZES. Long-term follow-up is essential for pulmonary NET G2 owing to the risk of distant metastasis and/or multiplicity. Moreover, the variability of menin IHC in MEN1-related tumors may indicate the pattern of tumor formation rather than the diagnostic utility of menin in MEN1.
Endoplasmic reticulum stress related genome-wide Mendelian randomization identifies therapeutic genes for ulcerative colitis and Crohn’s disease
Authors: Zou Menglong , Liang Qiaoli , Zhang Wei , Zhu Ying , Xu Yin 
Published: October 2023

Background: Endoplasmic reticulum stress (ERS) is an important pathophysiological mechanism in ulcerative colitis (UC) and Crohn’s disease (CD). ERS-related genes may be influenced by genetic factors and intestinal inflammation. However, the role of ERS as a trigger or potential etiological factor for UC and CD is unclear, as the expression of ERS-related genes in UC and CD may be the cause or subsequent changes in intestinal inflammation. Here, we used a three-step summary data-based Mendelian randomization (SMR) approach integrating multi-omics data to identify putative causal effects of ERS-related genes in UC and CD.Methods: Genome-wide association study (GWAS) summary data for UC (6,968 cases and 20,464 controls) and CD (5,956 cases and 14,927 controls) were extracted as outcome, and DNA methylation quantitative trait loci (mQTL, 1,980 participants) data and expression QTL data (eQTL, 31,684 participants) from the blood were obtained as exposure. The ERS-related genes were extracted from the GeneCards database, and then the GWAS summary data were integrated with the mQTL and eQTL data associated with ERS genes by SMR. Sensitivity analysis included two-sample MR analysis, power calculations, Bayesian co-localization analysis, and phenotype scanning were performed to evaluate the robustness of the results.Results: A total of 1,193 ERS-related genes were obtained. The three-step SMR analysis showed that cg24011261 CpG site regulating GPX1 expression was associated with a low risk of UC, whereas GPX1 expression regulated by a combination of cg05055782, cg24011261, and cg05551922 CpG sites was associated with a low risk of CD. Sensitivity analysis further supports these findings.Conclusion: This multi-omics integration study identifies a causal relationship between the role of ERS in UC and CD and suggests potential new therapeutic targets for clinical practice.
Causal relationship between Butyricimonas and allergic asthma: a two-sample Mendelian randomization study
Authors: Wan Wei , Qiu Yi , Huang Xinyi , Ren Yingxiang , Peng Andi , Yang Chunping 
Published: October 2023

BackgroundGrowing evidence has well documented the close association between the gut microbiome and allergic respiratory disease, which has been notably represented by allergic asthma. However, it is unclear whether this association is a causal link. Therefore, we investigated the potential causal associations between the gut microbiome and allergic asthma or other allergic diseases.MethodsIn this study, we performed two-sample Mendelian randomization (MR) analyses by using the publicly available genome-wide association study (GWAS) summary data. Single-nucleotide polymorphisms (SNPs) that significantly correlated were selected as instrumental variables. The inverse variance weighted (IVW) method was used to examine the potential causal gut microbial genera for allergic asthma and other allergic diseases. The robustness of the primary findings of the MR analyses was ensured by using different sensitivity analyses.ResultsCombining the findings from multiple analyses, the host genetic-driven increases in Butyricimonas at the genus level were positively correlated with the risk of allergic asthma. In addition, phylum Bacteroidetes and class Bacteroidia were also found to have negative associations with the risk of allergic asthma; genus Slackia was identified as having potential causal effects with allergic asthma. No clear evidence of pleiotropy and heterogeneity was observed in genus Butyricimonas. Butyricimonas was also found to have an association with allergic rhinitis, but not with other allergic diseases.ConclusionOur findings indicate that there are new gut microbial genera that were causally associated with the risk of allergic asthma and other allergic diseases, and offer novel insights into the pathogenesis of allergic respiratory diseases.
Graph analysis of cortical reorganization after virtual reality-based rehabilitation following stroke: a pilot randomized study
Authors: Feitosa Jamille Almeida , Casseb Raphael Fernandes , Camargo Alline , Brandao Alexandre Fonseca , Li Li Min , Castellano Gabriela 
Published: October 2023

IntroductionStroke is the leading cause of functional disability worldwide. With the increase of the global population, motor rehabilitation of stroke survivors is of ever-increasing importance. In the last decade, virtual reality (VR) technologies for rehabilitation have been extensively studied, to be used instead of or together with conventional treatments such as physiotherapy or occupational therapy. The aim of this work was to evaluate the GestureCollection VR-based rehabilitation tool in terms of the brain changes and clinical outcomes of the patients.MethodsTwo groups of chronic patients underwent a rehabilitation treatment with (experimental) or without (control) complementation with GestureCollection. Functional magnetic resonance imaging exams and clinical assessments were performed before and after the treatment. A functional connectivity graph-based analysis was used to assess differences between the connections and in the network parameters strength and clustering coefficient.ResultsPatients in both groups showed improvement in clinical scales, but there were more increases in functional connectivity in the experimental group than in the control group.DiscussionThe experimental group presented changes in the connections between the frontoparietal and the somatomotor networks, associative cerebellum and basal ganglia, which are regions associated with reward-based motor learning. On the other hand, the control group also had results in the somatomotor network, in its ipsilateral connections with the thalamus and with the motor cerebellum, which are regions more related to a purely mechanical activity. Thus, the use of the GestureCollection system was successfully shown to promote neuroplasticity in several motor-related areas.
Neuromodulation-induced prehabilitation to leverage neuroplasticity before brain tumor surgery: a single-cohort feasibility trial protocol
Authors: Boccuni Leonardo , Abellaneda-Pérez Kilian , Martín-Fernández Jesús , Leno-Colorado David , Roca-Ventura Alba , Prats Bisbe Alba , Buloz-Osorio Edgar Antonio , Bartrés-Faz David , Bargalló Nuria , Cabello-Toscano María , Pariente José Carlos , Muñoz-Moreno Emma , Trompetto Carlo , Marinelli Lucio , Villalba-Martinez Gloria , Duffau Hugues , Pascual-Leone Álvaro , Tormos Muñoz Josep María 
Published: October 2023

IntroductionNeurosurgery for brain tumors needs to find a complex balance between the effective removal of targeted tissue and the preservation of surrounding brain areas. Neuromodulation-induced cortical prehabilitation (NICP) is a promising strategy that combines temporary inhibition of critical areas (virtual lesion) with intensive behavioral training to foster the activation of alternative brain resources. By progressively reducing the functional relevance of targeted areas, the goal is to facilitate resection with reduced risks of neurological sequelae. However, it is still unclear which modality (invasive vs. non-invasive neuromodulation) and volume of therapy (behavioral training) may be optimal in terms of feasibility and efficacy.Methods and analysisPatients undertake between 10 and 20 daily sessions consisting of neuromodulation coupled with intensive task training, individualized based on the target site and neurological functions at risk of being compromised. The primary outcome of the proposed pilot, single-cohort trial is to investigate the feasibility and potential effectiveness of a non-invasive NICP protocol on neuroplasticity and post-surgical outcomes. Secondary outcomes investigating longitudinal changes (neuroimaging, neurophysiology, and clinical) are measured pre-NICP, post-NICP, and post-surgery.Ethics and disseminationEthics approval was obtained from the Research Ethical Committee of Fundació Unió Catalana d'Hospitals (approval number: CEI 21/65, version 1, 13/07/2021). The results of the study will be submitted to a peer-reviewed journal and presented at scientific congresses.Clinical trial registrationClinicalTrials.gov, identifier NCT05844605.
The many faces of gastrointestinal dysfunction in stiff person syndrome spectrum disorders
Authors: Koshorek Jacqueline , Wang Yujie , Maldonado Daniela Pimentel , Reyes-Mantilla Maria I. , Obando Danielle , Balshi Alexandra , Comisac Michael , Pasricha Pankaj Jay , Newsome Scott D. 
Published: October 2023

IntroductionThe effect of stiff person syndrome spectrum disorders (SPSD) on the gastrointestinal tract (GIT) is unknown. This case series aims to characterize the prevalence and types of GI dysfunction in individuals with SPSD.MethodsA retrospective chart review included individuals diagnosed with SPSD with descriptors of GI symptoms in their medical records. SPSD phenotypes, type of motility test performed, and dysmotility pattern (upper, lower, or diffuse) were assessed. Descriptive statistics and univariate chi-square analyses were utilized.ResultsOf 240 individuals with SPSD, 32% reported GI symptoms, most were female (83.1%), and white (74%), with a median age at time of GI symptom onset of 50 ± 13 years. Most common symptoms reported were dysphagia (45%), constipation (40%), and nausea/vomiting (23%). Most individuals had classic SPS (47%) followed by SPS-plus (29%) and 82.9% were positive for serum antiGAD65 antibodies. Of 36 patients that underwent at least one GI motility test, 26 had evidence of upper, lower, or diffuse GI dysmotility (44.4%, 17%, and 4%, respectively). The group who did not undergo testing had a higher proportion of patients with DM.DiscussionThere is a high prevalence of GI symptoms and transit abnormalities in patients with SPSD. Future prospective, longitudinal studies are warranted to further assess GI symptoms in the context of SPSD and to determine if individuals with GI symptoms differ in prognosis or treatment response from those without GI symptoms. In the meantime, there should be a low threshold for motility testing in patients with SPSD.
Physiological and cognitive changes after treatments of cyclophosphamide, methotrexate, and fluorouracil: implications of the gut microbiome and depressive-like behavior
Authors: Corley Christa , McElroy Taylor , Sridharan Bhavana , Trujillo Madison , Simmons Pilar , Kandel Sangam , Sykes Delawrence J. , Robeson Michael S. , Allen Antiño R. 
Published: October 2023

IntroductionChemotherapy-induced cognitive impairment colloquially referred to as chemobrain is a poorly understood phenomenon affecting a highly variable proportion of patients with breast cancer. Here we investigate the association between anxiety and despair-like behaviors in mice treated with cyclophosphamide, methotrexate, and fluorouracil (CMF) along with host histological, proteomic, gene expression, and gut microbial responses.MethodsForced swim and sociability tests were used to evaluate depression and despair-like behaviors. The tandem mass tag (TMT) proteomics approach was used to assess changes in the neural protein network of the amygdala and hippocampus. The composition of gut microbiota was assessed through 16S rRNA gene sequencing. Finally, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate changes in intestinal gap junction markers.Results and discussionWe observed that CMF induced social and despair-like behavior in mice 96 hours following treatment. Proteomic analysis identified changes in various proteins related to progressive neurological disease, working memory deficit, primary anxiety disorder, and gene expression revealing increases in NMDA and AMPA receptors in both the hippocampus and the amygdala because of CMF treatment. These changes finally, we observed immediate changes in the microbial population after chemotherapy treatment, with a notable abundance of Muribaculaceae and Romboutsia which may contribute to changes seen in the gut.