bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fertilization can accelerate the pace of soil microbial community response to rest‐grazing duration in the three‐river source region of China
Authors: Zhou Xuanbo , Wang Xiaoli , Ma Yushou , Wang Yanlong , Ma Yuan , Xie Lele 
Published: November 2023

AbstractOvergrazing leads to grassland degradation and productivity decline. Rest‐grazing during the regreen‐up period can quickly restore grassland and fertilization is a common restoration strategy. However, the effects of rest‐grazing time and fertilization on soil microorganisms are unclear in the alpine grasslands. Therefore, the experiment of rest‐grazing time and fertilization was carried out to explore the response of soil microorganisms to rest‐grazing time and fertilization measures. A field control experiment with rest‐grazing time and fertilization as factors have been conducted from the time when grass returned to green till the livestock moved to the summer pasture in Dawu Town of Maqin County of China. The primary treatment we established was the five rest‐grazing time, including rest‐grazing time of 20 days, 30 days, 40 days, 50 days, and traditional grazing was used as a check group. At the same time, the secondary treatment was nitrogen addition of 300 kg·hm−2 in each primary treatment. The results showed that the total phospholipid fatty acid (total PLFA), actinomyces (Act), and arbuscular mycorrhizal fungi (AMF) showed an ever‐increasing biomass with the increase of rest‐grazing time and the highest was at 50 days of rest‐grazing, and they were all significantly higher than CK. In addition, soil microbial biomass carbon‐nitrogen ratio (MBC/MBN) had great influence on the change of microbial community. Applying nitrogen fertilizer can increase the maximum value of biomass of all PLFA groups and the biomass of all PLFA groups changed in an “inverted V” shape with the increase of rest‐grazing time. Besides, instead of MBC/MBN, NO3−‐N was positively correlated with the biomass of all PLFA groups, which actively regulated the trend of microbial functions. The longer rest‐grazing time is more conducive to the biomass of all PLFA groups. However, applying nitrogen fertilizer could break this pattern, namely, the 30 days rest‐grazing would be beneficial to the biomass of all PLFA groups. These findings provide key information that rest‐grazing during the regreen‐up period is benefiscial to the all PLFA groups and fertilization could change the response of microorganisms to rest‐grazing, which provide reference measures for the restoration of degraded alpine meadows.
Parental exposure to heat waves improves offspring reproductive investment in Tetranychus urticae (Acari: Tetranychidae), but not in its predator, Phytoseiulus persimilis (Acari: Phytoseiidae)
Authors: Tscholl Thomas , Nachman Gösta , Spangl Bernhard , Scalmani Ida , Walzer Andreas 
Published: November 2023

AbstractThe more frequent and intense occurrence of heat waves is a challenge for arthropods because their unpredictable incidence requires fast adaptations by the exposed individuals. Phenotypic plasticity within and across generations might be a solution to cope with the detrimental effects of heat waves, especially for fast‐developing, small arthropods with limited dispersal abilities. Therefore, we studied whether severe heat may affect the reproduction of a pest species, the spider mite Tetranychus urticae, and its counterpart, the predatory mite Phytoseiulus persimilis. Single offspring females with different parental thermal origins (reared under mild or extreme heat waves) of both species were exposed to mild or extreme heat waves on bean leaves over 10 days, and the oviposition, egg sizes, survival, and escape behavior of the females were evaluated daily. The total losses of predators mainly via escapers were very high compared to prey, which makes a separation between selective and plastic effects on shifted reproductive traits impossible. Predator females laid smaller eggs, while their consumption and oviposition rates were unaffected during extreme heat waves. In comparison, larger prey females fed more and produced more, but smaller, eggs due to within‐ and trans‐generational effects. These advantages for the prey in comparison to its predator when exposed to extreme heat waves during the reproductive phase support the trophic sensitivity hypothesis: higher trophic levels (i.e., the predator) are more sensitive to thermal stress than lower trophic levels (i.e., the prey). Furthermore, the species‐specific responses may reflect their lifestyles. The proactive and mobile predator should be selected for behavioral thermoregulation under heat waves via spatiotemporal avoidance of heat‐exposed locations rather than relying on physiological adaptations in contrast to the more sessile prey. Whether these findings also influence predator–prey interactions and their population dynamics under heat waves remains an open question.
Hillslope to channel hydrologic connectivity in a dryland ecosystem
Authors: Keller Zachary T. , Vivoni Enrique R. , Kimsal Charles R. , Robles‐Morua Agustín , Pérez‐Ruiz Eli R. 
Published: November 2023

AbstractHydrologic connectivity refers to the processes and thresholds leading to water transport across a landscape. In dryland ecosystems, runoff production is mediated by the arrangement of vegetation and bare soil patches on hillslopes and the properties of ephemeral channels. In this study, we used runoff measurements at multiple scales in a small (4.67 ha) mixed shrubland catchment of the Chihuahuan Desert to identify controls on and thresholds of hillslope‐channel connectivity. By relating short‐ and long‐term hydrologic records, we also addressed whether observed changes in outlet discharge since 1977 were linked to modifications in hydrologic connectivity. Hillslope runoff production was controlled by the maximum rainfall intensity occurring in a 30‐min interval (I30), with small‐to‐negligible effects of antecedent surface soil moisture, vegetation cover, or slope aspect. An I30 threshold of nearly 10 mm/h activated runoff propagation from the shrubland hillslopes and through the main ephemeral channel, whereas an I30 threshold of about 16 mm/h was required for discharge from the catchment outlet. Since storms rarely exceed I30, full hillslope‐channel connectivity occurs infrequently in the mixed shrubland, leading to <2% of the annual precipitation being converted into outlet discharge. Progressive decreases in outlet discharge since 1977 could not be explained by variations in precipitation metrics, including I30, or the process of woody plant encroachment. Instead, channel modifications from the buildup of sediment behind measurement flumes may have increased transmission losses and reduced outlet discharge. Thus, alterations in channel properties can play an important role in the long‐term (45‐year) variations of rainfall–runoff dynamics of small desert catchments.
Proportion of charcoal carbon in the forest floor carbon pool in relation to fire history in a boreal forest landscape
Authors: Haukenes Vilde L. , Asplund Johan , Åsgård Lisa , Rolstad Jørund , Storaunet Ken Olaf , Ohlson Mikael 
Published: November 2023

AbstractFire in the boreal forests emits substantial amounts of organically bound carbon (C) to the atmosphere and converts a fraction of the burnt organic matter into charcoal, which in turn is highly refractory and functions as a long‐term stable C pool. It is well established that the boreal forest charcoal pool is sufficiently large to play a significant role in the global C cycle. However, there is a need for spatially representative estimates of how large proportions of the forest floor C pool are made up of charcoal across different plant communities in the boreal forest ecosystem. Thus, we have quantified the amounts of C separately in charcoal and the organic layers of the forest floor across fine spatial scales in a boreal forest landscape with a well‐documented fire history. We found that the proportion of charcoal C made up an average of 1.2% of the total forest floor C, and the charcoal proportions showed a high small‐scale spatial variability and were concentrated in the organic–mineral soil interface. Proportions of charcoal C decreased with increasing time since last fire. Deeper soils, denser soils, and local concave areas had the highest proportions of charcoal C, whereas historical fire frequencies and current differences in vegetation did not relate to the proportions of charcoal C.
MFishBT: A global database of biogeochemical tags in migratory fish
Authors: Ding Liuyong , Ding Chengzhi , Tao Juan , Avigliano Esteban , Shipley Oliver N. , Tang Bangli , Chen Jinnan , Liu Xingchen , Sun Jingrui , He Daming 
Published: November 2023

AbstractHumans have long been fascinated by the mysteries surrounding fish migrations and addressing these complex behaviors often requires large data sets. Biogeochemical tags, including trace elements and stable isotopes, are the most accessible biomarkers for tracking fish migrations. However, access to standardized biogeochemical tag data is rarely available for migratory fish, which limits our understanding of the evolutionary origins, drivers, timing, and corridors of migration. This precludes the development of conservation strategies and the implementation of management actions. Here, we present MFishBT, a global, open‐access database of Migratory Fish's Biogeochemical Tags. As of April 2023, the MFishBT contains biogeochemical records from 1,305 studies, of which 53% used element‐to‐calcium (E/Ca) ratios, 34% used isotopic ratios, and 13% used both. The database covers 17,413 field sampling locations (inland 47% vs. marine 53%) around the globe, comprising 490 migratory fish species of four classes, 44 orders/suborders, and 137 families. In total, 77 trace elements and 11 isotope systems were measured across various fish biological archives, including otoliths, scales, eye lenses, and vertebrae. E/Ca ratios were examined more frequently than isotopic ratios, led by Sr/Ca, Mg/Ca, Ba/Ca, and 87Sr/86Sr, δ13C, and δ18O. The MFishBT compiles 27,030, 16,222, and 2,481,714 records with biogeochemical data detected in the core, edge, and core‐to‐edge transects for biological archives of migratory fish. This is the most globally comprehensive open‐access database on biogeochemical tags in migratory fish to date, and can serve a variety of needs in scientific research, conservation, and management. We encourage researchers to add more data sets to this database in the future. This database is released for noncommercial use only. There are no copyright restrictions, and please cite this paper when using these data, or a subset of these data, for publication.
Environmental DNA biomonitoring in biodiversity hotspots: A case study of fishes of the Okavango Delta
Authors: von der Heyden Sophie , Neef Götz , Grevesse Thomas , Cwecwe Yandisa , Sado Tetsuya , Miya Masaki , Mosie Ineelo , Creer Simon , Skelton Paul , von Brandis Rainer 
Published: November 2023

AbstractThe Okavango Delta is the largest freshwater wetland in southern Africa and a recognized biodiversity hotspot and UNESCO World Heritage Site. The region is extremely rich in floral and faunal diversity, including a fish fauna of ~90 species in 15 families, that also support recreational and subsistence fishing. Anthropogenic pressures and invasive species threaten the unique biodiversity and ecosystem services that the Delta provides, necessitating biomonitoring tools that can provide broad community‐level diversity insights. Here, we utilize environmental DNA metabarcoding of aquatic eDNA using the MiFish 12S rRNA primers, to investigate fish communities and also sequenced 211 mtDNA 12S barcodes for 74 species across 36 genera of fishes from the region. Metabarcoding recovered 11 of 15 families, with 40 species detected across 23 genera, representing ~50% of known diversity, with the mtDNA 12S fragment able to delineate all genera (except for the cichlid genera Serranochromis and Pharyngochromis that comprised a single clade) and most species, except for some in the Clarias, Enteromius, Labeo, Lacustricola, and Petrocephalus genera. Generally, abundant and wide‐spread taxa such as Clarias spp. and Marcusenius altisambesi, amongst others, were often detected in the surveys, with other species, including Zaireichthys kavangoensis, Schilbe intermedius, and Labeo sp. detected less frequently. Dissolved oxygen, temperature, and dissolved organic solids were positively correlated with community diversity, highlighting the influence of environmental factors in shaping fish communities in the region. Further, there was strong variability in the eDNA signal across only 1000 m, suggesting that future surveys need to consider spatio‐temporal aspects of sample collection. Our study highlights the potential of eDNA metabarcoding for surveying aquatic biodiversity in the Okavango Delta, particularly within the context of baseline biodiversity inventories, that underpin conservation and management initiatives. As such, we provide a number of recommendations that can help structure future sampling efforts in the region.
The efficacy and safety of new potassium binders on renin–angiotensin–aldosterone system inhibitor optimization in heart failure patients: a systematic review and meta‐analysis
Authors: Abuelazm Mohamed , Badr Amr , Turkmani Mustafa , Amin Mostafa Atef , Amin Ahmed Mazen , Aboutaleb Aya , Gowaily Ibrahim , Soliman Youssef , Abdelazeem Basel 
Published: November 2023

AbstractGuideline‐directed medical therapy (GDMT) has improved outcomes in patients with heart failure, including the use of renin–angiotensin–aldosterone system inhibitors, which can hinder the excretion of potassium, resulting in hyperkalaemia. New potassium binders (NPBs) can prevent this adverse effect; however, the efficacy and safety of NPB for this indication have not been fully established. We conducted a systematic review and meta‐analysis synthesizing randomized controlled trials (RCTs), which were retrieved by systematically searching PubMed, Web of Science, Scopus, and Cochrane through 26 April 2023. The risk of bias assessment was conducted, following Cochrane's updated Risk of Bias 2 assessment tool. We used the fixed‐effects model to pool dichotomous data using risk ratio (RR) and continuous data using mean difference (MD), with a 95% confidence interval (CI) (PROSPERO ID: CRD42023426113). We included six RCTs with a total of 1432 patients. NPB was significantly associated with successful mineralocorticoid receptor antagonist (MRA) optimization [RR: 1.13 with 95% CI (1.02–1.25), P = 0.02], decreased patients with MRA at less than the target dose [RR: 0.72 with 95% CI (0.57–0.90), P = 0.004], and decreased hyperkalaemic episodes [RR: 0.42 with 95% CI (0.24–0.72), P = 0.002]. However, there was no difference between NPB and placebo regarding angiotensin‐converting enzyme inhibitor (ACEi)/angiotensin receptor blocker (ARB)/angiotensin receptor/neprilysin inhibitor (ANRi) optimization [RR: 1.02 with 95% CI (0.89–1.17), P = 0.76] and serum potassium change [MD: −0.31 with 95% CI (−0.61 to 0.00), P = 0.05], with an acceptable safety profile except for the increased incidence of hypokalaemia with NPB [RR: 1.57 with 95% CI (1.12–2.21), P = 0.009]. NPB has been shown to improve GDMT outcomes by enhancing MRA optimization and reducing hyperkalaemic episodes. However, there are limited data on the effects of NPB on ACEi/ARB/ANRi optimization. Future RCTs should investigate ACEi/ARB/ANRi optimization and conduct head‐to‐head comparisons of NPB (patiromer and sodium zirconium cyclosilicate).
Heart failure ‘the cancer of the heart’: the prognostic role of the HLM score
Authors: Severino Paolo , Mancone Massimo , D'Amato Andrea , Mariani Marco Valerio , Prosperi Silvia , Alunni Fegatelli Danilo , Birtolo Lucia Ilaria , Angotti Danilo , Milanese Alberto , Cerrato Enrico , Maestrini Viviana , Pizzi Carmine , Foà Alberto , Vestri Annarita , Palazzuoli Alberto , Vizza Carmine Dario , Casale Paul N. , Mather Paul J. , Fedele Francesco 
Published: November 2023

AbstractAimsThe multi‐systemic effects of heart failure (HF) resemble the spread observed during cancer. We propose a new score, named HLM, analogous to the TNM classification used in oncology, to assess the prognosis of HF. HLM refers to H: heart damage, L: lung involvement, and M: systemic multiorgan involvement. The aim was to compare the HLM score to the conventional New York Heart Association (NYHA) classification, American College of Cardiology/American Heart Association (ACC/AHA) stages, and left ventricular ejection fraction (LVEF), to assess the most accurate prognostic tool for HF patients.Methods and resultsWe performed a multicentre, observational, prospective study of consecutive patients admitted for HF. Heart, lung, and other organ function parameters were collected. Each patient was classified according to the HLM score, NYHA classification, ACC/AHA stages, and LVEF assessed by transthoracic echocardiography. The follow‐up period was 12 months. The primary endpoint was a composite of all‐cause death and rehospitalization due to HF. A total of 1720 patients who completed the 12 month follow‐up period have been enrolled in the study. 520 (30.2%) patients experienced the composite endpoint of all‐cause death and rehospitalization due to HF. 540 (31.4%) patients were female. The mean age of the study population was 70.5 ± 12.9. The mean LVEF at admission was 42.5 ± 13%. Regarding the population distribution across the spectrum of HLM score stages, 373 (21.7%) patients were included in the HLM‐1, 507 (29.5%) in the HLM‐2, 587 (34.1%) in the HLM‐3, and 253 (14.7%) in the HLM‐4. HLM was the most accurate score to predict the primary endpoint at 12 months. The area under the receiver operating characteristic curve (AUC) was greater for the HLM score compared with the NYHA classification, ACC/AHA stages, or LVEF, regarding the composite endpoint (HLM = 0.645; NYHA = 0.580; ACC/AHA = 0.589; LVEF = 0.572). The AUC of the HLM score was significantly better compared with the LVEF (P = 0.002), ACC/AHA (P = 0.029), and NYHA (P = 0.009) AUC.ConclusionsThe HLM score has a greater prognostic power compared with the NYHA classification, ACC/AHA stages, and LVEF assessed by transthoracic echocardiography in terms of the composite endpoint of all‐cause death and rehospitalization due to HF at 12 months of follow‐up.
Cerebellar somatotopy of the trigemino‐cervical complex during nociception
Authors: Basedau Hauke , May Arne , Mehnert Jan 
Published: November 2023

AbstractIntroductionThe somatotopic organization of the human cerebellum processes somato‐motoric input. Its role during pain perception for nociceptive input remains ambiguous. A standardized experimental trigeminal nociceptive input in functional imaging might clarify the role of the cerebellum in trigeminal nociception. Also of interest is the greater occipital nerve, which innervates the back of the head, and can influence the trigeminal perception due to functional coupling within the brainstem, forming the so‐called trigemino‐cervical complex.MethodsIn our preregistered study (clinicaltrials.gov: NTC03999060), we stimulated the greater occipital as well as the three main branches of the trigeminal nerve during functional magnetic resonance imaging in two independent cohorts of young healthy volunteers without psychiatric, neurological or pain‐related disorders to disentangle overlapping somatotopic cerebellar organization of the nerves innervating the human head.ResultsWe found a dominant effect of the first trigeminal branch in the cerebellum, underpinning its particular role for headache diseases, and somatotopic representations in bilateral cerebellar lobules I–IV, V, VIIb, VIIIa and Crus I as well as in the brainstem.SignificanceThe study expands the current knowledge on facial and head pain processing by the cerebellum and provides an initial somatotopic map of the trigemino‐cervical complex in the human cerebellum with a predominant representation of the first trigeminal branch.
Dual‐Objective Optimization for Maximizing Photovoltaic Output Power in a Two‐Stage Single‐Phase Grid‐Tied System
Authors: K.T. Swetha , Reddy Barry Venugopal , Jain Rohit Kumar 
Published: November 2023

The effective utilization of photovoltaic systems (PVS) is significantly affected by partial shading (PS). Although total cross‐tied topology minimizes the power loss, the partial shading condition (PSC) decreases the output power generation and exhibits multiple power peaks in the Power Voltage characteristics. To address this issue, a dual‐objective optimization technique based on reduced search space equilibrium optimization (RSS‐EO) for a single‐phase, two‐stage grid‐connected PVS, is presented. The proposed technique accomplishes two objectives 1) maximizing power generation by reconfiguring the PV modules using a switching matrix based on irradiance equalization and 2) extracting the global maximum power (GMP) with faster convergence, negligible steady‐state oscillations, and reduced steady‐state error. The simulation and experimental results validate the effectiveness of the proposed RSS‐EO technique in efficiently distributing shade over the entire PV array and tracking GMP. The power enhancements achieved by RSS‐EO, particle swarm optimization, and Munkres are 23.03%, 20.02%, and 19.01%, respectively. Additionally, the proposed method exhibits an average mismatch power loss of 738 W, a fill factor of 58.1%, and a percentage power loss of 26.83%. Furthermore, the proposed algorithm is evaluated based on energy calculations and shows better performance by achieving the shortest payback period of 1.6 years.
DYNATE: Localizing rare‐variant association regions via multiple testing embedded in an aggregation tree
Authors: Li Xuechan , Pura John , Allen Andrew , Owzar Kouros , Lu Jianfeng , Harms Matthew , Xie Jichun 
Published: November 2023

AbstractRare‐variants (RVs) genetic association studies enable researchers to uncover the variation in phenotypic traits left unexplained by common variation. Traditional single‐variant analysis lacks power; thus, researchers have developed various methods to aggregate the effects of RVs across genomic regions to study their collective impact. Some existing methods utilize a static delineation of genomic regions, often resulting in suboptimal effect aggregation, as neutral subregions within the test region will result in an attenuation of signal. Other methods use varying windows to search for signals but often result in long regions containing many neutral RVs. To pinpoint short genomic regions enriched for disease‐associated RVs, we developed a novel method, DYNamic Aggregation TEsting (DYNATE). DYNATE dynamically and hierarchically aggregates smaller genomic regions into larger ones and performs multiple testing for disease associations with a controlled weighted false discovery rate. DYNATE's main advantage lies in its strong ability to identify short genomic regions highly enriched for disease‐associated RVs. Extensive numerical simulations demonstrate the superior performance of DYNATE under various scenarios compared with existing methods. We applied DYNATE to an amyotrophic lateral sclerosis study and identified a new gene, EPG5, harboring possibly pathogenic mutations.