bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studying the Structure and Evaluating α‐Glucosidase Inhibition of Novel Acetamide Derivatives Incorporating 4‐Ethyl‐4H‐1,2,4‐Triazole Starting from 2‐(Naphthalen‐1‐yl)Acetic Acid
Authors: Le Trong Duc , Nguyen Tien Cong , Tran Thi Minh Dinh , Hoang Thi Kim Dung , To Khanh Duong , Nguyen Minh Phuong Hien , Tran Thi My Ngan , Dinh Chau Phi , Alhaji Jibril Abdullahi , Van Meervelt Luc 
Published: February 2024

AbstractThis research aims to create innovative compounds that incorporate 1,2,4‐triazole and exhibit α‐glucosidase inhibitory potential. Similar to our previously reported series of N‐aryl 2‐{[5‐(naphthalen‐1‐ylmethyl)‐4‐phenyl‐4H‐1,2,4‐triazol‐3‐yl]thio}acetamide compounds, we explore here 4‐ethyl instead of 4‐phenyl as substituent. The synthesis process effectively yielded these compounds, with the highest yield reaching up to 91 % for compound N‐phenyl‐2‐{[5‐(naphthalen‐1‐ylmethyl)‐4‐ethyl‐4H‐1,2,4‐triazol‐3‐yl]thio}acetamide 5 a. Their structures were validated through various spectroscopic techniques such as IR, 1H‐NMR, 13C‐NMR, and HR‐MS spectra, and for compounds 3, 5 d, and 5 e by X‐ray diffraction. In vitro experiments revealed that only compound 5 g, marked by a 3‐hydroxy substitution on the N‐phenylacetamide moiety, demonstrated higher α‐glucosidase inhibitory potential (IC50=158.4±3.4 μM) compared to the positive control, acarbose (IC50=351.3±1.8 μM). Molecular docking studies also coincide with in vitro assay by uncovering a strong hydrogen bond with residue Asp1526 along with other hydrophobic interactions of compound 5 g in the α‐glucosidase binding pocket. Compound 5 g showed a free binding energy of −9.7 kcal/mol, contrasting with acarbose (−8.0 kcal/mol). Despite the modest biological activity, this research underscores the simplicity and convenience of the procedure for synthesizing 1,2,4‐triazole‐based compounds, and contributes a key feature to the structure‐activity relationship of the triazole scaffold in the α‐glucosidase pocket.
A novel small‐animal locomotor activity recording device for biological clock research
Authors: Wu Yi‐Long , Zhong Ming , Yin Jun , Ou Wei‐Jie , Zhuang Yu‐Bin , Zhang Nan‐Wen , Lin Su , Zhu Yue‐Yong 
Published: February 2024

AbstractThe rodent running‐wheel recording apparatus is a reliable approach for studying circadian rhythm. This study demonstrated how to construct a simple and intelligent running‐wheel recording system. The running wheel was attached to the cage's base, whereas the Hall sensor was attached to the cage's cover. Then, the RJ25 adaptor relayed the running signal to the main control board. Finally, the main control board was connected to the USB port of the computer with the USB connection. Data were collected using the online‐accessible, self‐created software Magturning. Through Magturning, generated data were saved and exported in real time. Afterward, the device was validated by collecting data on the locomotor activities of mice under different light conditions. In conclusion, this new device can record circadian activity of rodents. Our device is appropriate for interdisciplinary investigations related to biological clock research.
Peptide Stapling by Crosslinking Two Amines with α‐Ketoaldehydes through Diverse Modified Glyoxal‐Lysine Dimer Linkers
Authors: Guo Pan , Chu Xin , Wu Chengjin , Qiao Tianjiao , Guan Wenli , Zhou Chuanzheng , Wang Tao , Tian Changlin , He Gang , Chen Gong 
Published: February 2024

Abstractα‐Ketoaldehydes play versatile roles in the ubiquitous natural processes of protein glycation. However, leveraging the reactivity of α‐ketoaldehydes for biomedical applications has been challenging. Previously, the reactivity of α‐ketoaldehydes with guanidine has been harnessed to design probes for labeling Arg residues on proteins in an aqueous medium. Herein, a highly effective, broadly applicable, and operationally simple protocol for stapling native peptides by crosslinking two amino groups through diverse imidazolium linkers with various α‐ketoaldehyde reagents is described. The use of hexafluoroisopropanol as a solvent facilitates rapid and clean reactions under mild conditions and enables unique selectivity for Lys over Arg. The naturally occurring GOLD/MOLD linkers have been expanded to encompass a wide range of modified glyoxal‐lysine dimer (OLD) linkers. In a proof‐of‐concept trial, these modular stapling reactions enabled a convenient two‐round strategy to streamline the structure–activity relationship (SAR) study of the wasp venom peptide anoplin, leading to enhanced biological activities.
Dynamic Modulation of Li2O2 Growth in Li‐O2 Batteries through Regulating Oxygen Reduction Kinetics with Photo‐Assisted Cathodes
Authors: Yu Haohan , Liu Dapeng , Fu Zerui , Wang Shu , Zuo Xintao , Feng Xilan , Zhang Yu 
Published: February 2024

AbstractWidely acknowledged that the capacity of Li‐O2 batteries (LOBs) should be strongly determined by growth behaviors of the discharge product of lithium peroxide (Li2O2) that follows both coexisting surface and solution pathways. However until now, it remains still challenging to achieve dynamic modulation on Li2O2 morphologies. Herein, the photo‐responsive Au nanoparticles (NPs) supported on reduced oxide graphene (Au/rGO) have been utilized as cathode to manipulate oxygen reduction reaction (ORR) kinetics by aid of surface plasmon resonance (SPR) effects. Thus, we can experimentally reveal the importance of matching ORR kinetics with Li+ migration towards battery performance. Moreover, it is found that Li+ concentration polarization caused “sudden death” of LOBs is supposed to be just a form of suspended animation that could timely recover under irradiation. This work provides us an in‐depth explanation on the working mechanism of LOBs from a kinetic perspective, offering valuable insights for the future battery design.
Antibiotic Resistance Detection in Pseudomonas aeruginosa: Recent Strategies, Advances, and Challenges
Authors: Clarindo Lopes Luma , Koushanpour Ashkan , Wiebe Kolby , Kuss Sabine 
Published: February 2024

AbstractAntibiotic resistance presents a worldwide public health emergency, impeding the effective management of infectious diseases. Pseudomonas aeruginosa plays a substantial role as a bacterial pathogen, particularly in infections among hospitalized individuals, and those with weakened immune systems. Timely and accurate detection of antimicrobial resistance in P. aeruginosa is crucial for initiating tailored antibiotic therapy promptly, thus improving patient outcomes. Nevertheless, this endeavor encounters challenges due to the intricate and varied nature of antibiotic‐resistant strains. Extensive efforts have been invested in developing sensors and instrumentations for assessing antibiotic resistance or susceptibility (AST), aiming to enable personalized patient treatment with appropriate medications. This paper focuses on recent advancements in these methodologies, utilized for evaluating the degree of antibiotic resistance or susceptibility in pathogenic bacteria. Flow cytometry, dual molecular recognition and mass spectrometry are presented as newer phenotypic AST techniques. Genomic methods and the pyocyanin detection are listed as methodologies for the detection of resistance indicators.
Fabrication of stretchable and high‐filtration performance melt‐blown nonwoven webs for PM0.3 aerosol filtration
Authors: Eticha Andinet Kumella , Akgul Yasin , Pakolpakcil Ayben , Unlu Oğuz Kagan , Cug Harun , Kilic Ali 
Published: February 2024

AbstractPolypropylene (PP) is a semi‐crystalline polymer that displays simple manufacturing, high stiffness, lightweight, chemical resistance, and inexpensive. However, PP has significant drawbacks, such as poor brittleness at low temperatures, high shrinkage ratio, and low impact resistance, which limit its development. Thermoplastic polyurethane (TPU) possesses recyclable and eco‐friendly characteristics, along with the elasticity of rubber and exceptional mechanical properties. In this study, a flexible and high‐filtration performance PP‐TPU textile material was developed by melt‐blowing for filtering PM0.3 aerosols. For the first time, a melt‐blown PP‐TPU nonwoven was used as an air filter. The fiber morphological studies exhibited that addition of 10 and 20 wt.% TPU into PP resulted in a fiber diameter increment from 0.94 to 1.24 μm. Also, melt‐blown PP‐TPU forms helical fibers, which are different from fibers noticed in melt‐blown PP. Corona‐charged double‐layer 80PP‐20TPU nonwovens have a filtration efficiency of 99.25% and quality factor (QF) of 0.13 mm H2O−1 at an air flow rate of 95 L/min. Moreover, PP's tensile strength was increased by 72.22%, and elongation was raised by 38.1% with the addition of 20 wt.% TPU. Thus, PP‐TPU melt‐blown composites may bring novel perspectives into the design and development of high‐performance filtering materials for a variety of applications.
Treating cardiac tamponade due to malignant effusion with percutaneous balloon pericardiotomy: Back to the future
Authors: Chaus Adib , Uretsky Barry F. 
Published: February 2024

Key points
Percutaneous balloon pericardiotomy (PBP) involves creation of large hole in the pericardium with balloon inflation to avoid the need for a surgical “window” for treatment of malignant pericardial effusion.
The procedure can be safely performed effectively with low complication rate and it can be repeated for recurrent pericardial effusion.
The recurrence rate of pericardial effusion was 24.5% with a mean survival of 5.8 months. The subgroup surviving at least 1 month, likely had a longer median survival than the overall group, suggesting its value in a subgroup of patients.
Exposure to and Biomarker Responses From Legacy and Emerging Contaminants Along Three Drainages in the Milwaukee Estuary, Wisconsin, USA
Authors: Custer Christine M. , Custer Thomas W. , Dummer Paul M. , Schultz Sandra , Karouna-Renier Natalie , Tseng Chi Yen , Matson Cole W. 
Published: February 2024

Abstract
Legacy contaminants and contaminants of emerging concern (CECs) were assessed in tree swallow (Tachycineta bicolor) tissue and diet samples from three drainages in the Milwaukee estuary, Wisconsin, USA, to understand exposures and possible biomarker responses. Two remote Wisconsin lakes were assessed for comparative purposes. Bioaccumulative classes of contaminants, such as polybrominated diphenyl ethers and per- and polyfluoroalkyl substances, while at higher concentrations than the reference lakes, did not vary significantly among sites or among the three drainages. Polycyclic aromatic hydrocarbons were assessed in diet and sediment and were from primarily pyrogenic sources. Ten biomarkers were assessed relative to contaminant exposure. Polychlorinated biphenyls (PCBs) were elevated above reference conditions at all Milwaukee sites but did not correlate with any measured biomarker responses. Only one site, Cedarburg, just downstream from a Superfund site, had elevated PCBs compared to other sites in the Milwaukee estuary. Few non-organochlorine insecticides or herbicides were detected in tree swallow liver tissue, except for the atrazine metabolite desethylatrazine. Few pharmaceuticals and personal care products were detected in liver tissue except for N,N-diethyl-meta-toluamide, iopamidol, and two antibiotics. The present study is one of the most comprehensive assessments to date, along with the previously published Maumee River data, on the exposure and effects of a wide variety of CECs in birds. Environ Toxicol Chem 2024;43:856–877. © 2024 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Concentrations of many little-studied contaminants were assessed in tree swallows in three drainages within the Milwaukee estuary, Wisconsin, USA.
Connectedness and risk spillover in China's commodity futures sectors
Authors: Long Jun , Yuan Xianghui , Jin Liwei , Zhao Chencheng 
Published: February 2024

AbstractThis study employs minimum spanning tree and generalized forecast error variance decomposition methods to investigate the connectedness and risk spillovers across China's commodity sectors from January 2016 to December 2021. The results show that total connectedness within the commodity system is time varying. Chemical is the main risk driver, while other sectors occasionally dominate the system. These two methods achieve consistent results in identifying the systemically important sector and dynamic connectedness. In addition, we find that Chinese economic policy uncertainty and the investor sentiment index have significant impacts on total connectedness. Our findings have implications for preventing systemic risk for policymakers and managing commodity portfolio risk for investors.
Large thermal heat storages in rock caverns – numerical simulation of heat losses
Authors: Messerklinger Sophie , Smaadahl Mikkel , Rabaiotti Carlo 
Published: February 2024

AbstractIn the future large energy storage facilities will play a key role in district heating systems that transport heat energy through tube systems with water as transport media. Energy storages enable storage of renewable energy and industrial waste heat through flexible buffer heat storage and allow a reduction in installed capacities of heat supply stations. In this article, the application of water‐filled rock caverns for the use of large thermal energy storages is analysed. A key issue is the energy loss over the month/year. Therefore, this study focuses on the quantification of energy losses from water‐filled rock caverns by means of numerical analysis. Three different rock cavern geometries are analysed, varying rock conductivity parameter and varying temperature profiles of the water storage. By simulations with the software COMSOL it could be shown that (i) the energy losses of underground caverns are only 25 % compared to the energy losses from the currently used insulated steel tanks located above ground, (ii) the energy losses can be further decreased by the application of a thermal insulation layer and (iii) the energy losses decrease over the lifetime due to the reducing temperature gradients in the surrounding rock. Since cavern reservoirs can be operated for more than 100 years, these findings are of great relevance and shall be further investigated with respect to economical assessment.
Compressed cerebellar functional connectome hierarchy in spinocerebellar ataxia type 3
Authors: Liu Xinyuan , Guo Jing , Jiang Zhouyu , Liu Xingli , Chen Hui , Zhang Yuhan , Wang Jian , Liu Chen , Gao Qing , Chen Huafu 
Published: February 2024

AbstractSpinocerebellar ataxia type 3 (SCA3) is an inherited movement disorder characterized by a progressive decline in motor coordination. Despite the extensive functional connectivity (FC) alterations reported in previous SCA3 studies in the cerebellum and cerebellar‐cerebral pathways, the influence of these FC disturbances on the hierarchical organization of cerebellar functional regions remains unclear. Here, we compared 35 SCA3 patients with 48 age‐ and sex‐matched healthy controls using a combination of voxel‐based morphometry and resting‐state functional magnetic resonance imaging to investigate whether cerebellar hierarchical organization is altered in SCA3. Utilizing connectome gradients, we identified the gradient axis of cerebellar hierarchical organization, spanning sensorimotor to transmodal (task‐unfocused) regions. Compared to healthy controls, SCA3 patients showed a compressed hierarchical organization in the cerebellum at both voxel‐level (p < .05, TFCE corrected) and network‐level (p < .05, FDR corrected). This pattern was observed in both intra‐cerebellar and cerebellar‐cerebral gradients. We observed that decreased intra‐cerebellar gradient scores in bilateral Crus I/II both negatively correlated with SARA scores (left/right Crus I/II: r = −.48/−.50, p = .04/.04, FDR corrected), while increased cerebellar‐cerebral gradients scores in the vermis showed a positive correlation with disease duration (r = .48, p = .04, FDR corrected). Control analyses of cerebellar gray matter atrophy revealed that gradient alterations were associated with cerebellar volume loss. Further FC analysis showed increased functional connectivity in both unimodal and transmodal areas, potentially supporting the disrupted cerebellar functional hierarchy uncovered by the gradients. Our findings provide novel evidence regarding alterations in the cerebellar functional hierarchy in SCA3.
Control of muscle satellite cell function by specific exercise‐induced cytokines and their applications in muscle maintenance
Authors: Guo Qian , Luo Qing , Song Guanbin 
Published: February 2024

AbstractExercise is recognized to play an observable role in improving human health, especially in promoting muscle hypertrophy and intervening in muscle mass loss‐related diseases, including sarcopenia. Recent rapid advances have demonstrated that exercise induces the release of abundant cytokines from several tissues (e.g., liver, muscle, and adipose tissue), and multiple cytokines improve the functions or expand the numbers of adult stem cells, providing candidate cytokines for alleviating a wide range of diseases. Muscle satellite cells (SCs) are a population of muscle stem cells that are mitotically quiescent but exit from the dormancy state to become activated in response to physical stimuli, after which SCs undergo asymmetric divisions to generate new SCs (stem cell pool maintenance) and commit to later differentiation into myocytes (skeletal muscle replenishment). SCs are essential for the postnatal growth, maintenance, and regeneration of skeletal muscle. Emerging evidence reveals that exercise regulates muscle function largely via the exercise‐induced cytokines that govern SC potential, but this phenomenon is complicated and confusing. This review provides a comprehensive integrative overview of the identified exercise‐induced cytokines and the roles of these cytokines in SC function, providing a more complete picture regarding the mechanism of SC homeostasis and rejuvenation therapies for skeletal muscle.
Processing the process: Reflections on genetic counselor‐led student supervision groups and practical tips for future facilitators
Authors: Florido Michelle E. , Giordano Jessica L. 
Published: February 2024

AbstractGenetic counseling graduate students face growth and challenges across a variety of axes both personally and professionally throughout their training. The formation of leader‐led supervision groups for second‐year genetic counseling students has created a safe space for students to give and receive feedback, process their positionality in complex clinical scenarios and the medical system at large, dissect psychosocial counseling theory, and share personal and professional experiences with the overall aim of supporting their growth. This work requires faculty facilitators who are invested in student growth and operate from a framework of empathy, humanism, curiosity, and vulnerability. The authors share their reflections on stepping into the facilitator role with no prior experience other than their work in clinical genetic counseling in varied practice settings. Common themes across four cohorts of students are presented along with reflections on facilitator growth, consideration of student developmental stages, and the parallel process between providing clinical services to clients and educating students. The authors hope to highlight the value of processing cases, inspire other genetic counselors to engage in this work, and normalize the experiences of those already running genetic counseling student process groups.
Suboptimal reliability of FIB‐4 and NAFLD‐fibrosis scores for staging of liver fibrosis in general population
Authors: Hazzan Rawi , Abu Ahmad Nur , Habib Abu Shqara , Saleh Igbaria , Ziv Neeman 
Published: February 2024

AbstractBackground and AimThe burden and incidence of liver cirrhosis are increasing worldwide. Early detection of liver fibrosis would help in early interventions and preventing the progression of fibrosis and cirrhosis. The accepted noninvasive markers for liver fibrosis staging, namely fibrosis‐4 (FIB‐4) and nonalcoholic fatty liver disease fibrosis score (NFS), have shown inconsistent performance for detecting the fibrosis stage. We aimed to evaluate the efficacy of FIB‐4 score and NFS for the detection of liver fibrosis in the general population.MethodsFrom a general population referred from a single, community‐based family‐physician clinic, we included study participants between the ages of 45 and 65 years, with no knowledge of liver disease and no record of alcohol consumption. Liver fibrosis was evaluated by the FIB‐4 score and NFS using shear wave elastography (SWE) or transient elastography (TE) measurements as a reference.ResultsA total of 76 participants (aged 61.5 ± 0.37 years, 33% females) were included in the study cohort. We observed a nonsignificant correlation between liver stiffness measurement (LSM) and FIB‐4 and NFS (r = 0.1, P = 0.37; r = 0.16, P = 0.15, respectively). Our results showed that only 5.2% with FIB‐4 >3.25 and 9.7% with NFS >0.675 had LSM >12 kPa. The compatibility of fibrosis staging was 55% between FIB‐4 score and LSM and only 18% between NFS and LSM.ConclusionWe found that FIB‐4 and NFS are unreliable tools for liver fibrosis estimation in the general population. There is a need for more reliable noninvasive methods for the early detection of liver fibrosis.
Impact of citrulline substitution on clinical outcome after liver transplantation in carbamoyl phosphate synthetase 1 and ornithine transcarbamylase deficiency
Authors: Aldrian Denise , Waldner Birgit , Vogel Georg F. , El‐Gharbawy Areeg H. , McKiernan Patrick , Vockley Jerard , Landau Yuval E. , Al Mutairi Fuad , Stepien Karolina M. , Kwok Anne Mei‐Kwun , Yıldız Yılmaz , Honzik Tomas , Kelifova Silvie , Ellaway Carolyn , Lund Allan M. , Mori Mari , Grünert Sarah C. , Scholl‐Bürgi Sabine , Zöggeler Thomas , Oberhuber Rupert , Schneeberger Stefan , Müller Thomas , Karall Daniela 
Published: February 2024

AbstractCarbamoyl phosphate synthetase 1 (CPS1) and ornithine transcarbamylase (OTC) deficiencies are rare urea cycle disorders, which can lead to life‐threatening hyperammonemia. Liver transplantation (LT) provides a cure and offers an alternative to medical treatment and life‐long dietary restrictions with permanent impending risk of hyperammonemia. Nevertheless, in most patients, metabolic aberrations persist after LT, especially low plasma citrulline levels, with questionable clinical impact. So far, little is known about these alterations and there is no consensus, whether l‐citrulline substitution after LT improves patients' symptoms and outcomes. In this multicentre, retrospective, observational study of 24 patients who underwent LT for CPS1 (n = 11) or OTC (n = 13) deficiency, 25% did not receive l‐citrulline or arginine substitution. Correlation analysis revealed no correlation between substitution dosage and citrulline levels (CPS1, p = 0.8 and OTC, p = 1). Arginine levels after liver transplantation were normal after LT independent of citrulline substitution. Native liver survival had no impact on mental impairment (p = 0.67). Regression analysis showed no correlation between l‐citrulline substitution and failure to thrive (p = 0.611) or neurological outcome (p = 0.701). Peak ammonia had a significant effect on mental impairment (p = 0.017). Peak plasma ammonia levels correlate with mental impairment after LT in CPS1 and OTC deficiency. Growth and intellectual impairment after LT are not significantly associated with l‐citrulline substitution.