bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water quality and fish health in the upper asahan river in supporting the sustainability of fish resources
Authors: Anggraeni D P , Ditya Y C , Makmur S , Makri  , Fatah K , Ali M 
Published: December 2023

Abstract
This research aims to analyze condition of the aquatic environment from the physical and chemical aspects of the waters and analyze the health of fish in supporting the sustainability of fish resources in the upper part of the Asahan River. Sampling was carried out by purposive sampling method at nine stations obtained in situ and secondary data. The results showed that in general the quality of the waters upstream of the Asahan River was below the second class of river water quality standard. However, it is necessary to monitor regularly the parameters of ammonia and oil content in the waters of the Asahan River so as not to exceed the second class of river water quality standard, because this had implications for the development of the parasite population of Alitropus sp. In addition, the emergence of a population of fish lice (Alitropus sp.) has also caused many fish that used to inhabit the upper reaches of the Asahan River, there is only one species, namely Asa Fish (Osteochillus vittatus) and freshwater lobster (Cherax quadricarinatus). Several physical and chemical parameters of the waters have a positive effect on the development of the Alitropus typus parasite, including temperature, brightness, ammonia and pH.
Feeding management of vannamei shrimp (Litopenaeus Vannamei) with immunostimulant addition
Authors: Daniel K , Sari P D W 
Published: December 2023

Abstract
Giving immunostimulants is one of the preventive measures against infectious diseases. This activity aims to know the feed management of vannamei shrimp with adding immunostimulants on the culture of vannamei shrimp. The parameter observed were Total Haemocyte Count (THC) to know the effect of immunostimulant. The value of total haemocytes in the DOC 31-35 was 15.87×106 cells/ml - 25.56 × 106 cells/ml. The second measurement of total haemocyte count was done for the vannamei shrimp in the DOC 59-63 and showed 14.58×106 cells/ml - 27.45×106 cells/ml.
The effectiveness of additional silage of fish ofal and bagasse fermentation on biomass and population of silk worm (Tubifex tubifex)
Authors: Ismawati H , Sulmartiwi L , Dewi N N 
Published: December 2023

Abstract
The purpose of this study was to determine the effect of giving catfish offal silage and sugarcane bagasse fermentation to the biomass The method used in this research is experimental using Completely Randomized Design (CRD). The treatments were 413 gram (100%) rice field mud, 206.5 gram rice field mud + 75% fish offal silage + 25% bagasse fermentation, 206.5 gram rice field mud + 50% fish offal silage + 50% bagasse fermentation, rice field mud 206.5 grams + 25% silage of fish offal + 75% bagasse fermentation. Each treatment was repeated five times. The results showed that the highest peak of silk worm biomass with 206.5 grams of rice field mud culture + 25% fish offal silage + 75% bagasse fermentation with an average of 22.25 grams and the lowest biomass was produced on 406 grams (100%) mud media. Rice fields with an average of 7.15 grams. The highest population of silkworms was found in 206.5 gram rice field mud media + 25% fish offal silage + 75% bagasse fermentation obtained 6651 ind, the lowest population in 406 gram (100%) rice field mud media with an average of 2.263 ind.
Water profile of white shrimp (Litopenaeus vannamei) infected with white feces disease (WFD)
Authors: Rahardjo K K E , Satyantini W H , Amin M , Mukti A T 
Published: December 2023

Abstract
In mid-2021, shrimp farmer reported case of mass deaths in white shrimp (Litopenaeus vannamei) in Pasuruan Regency, reaching 2.6-3 kg/day in Kalianyar Village, Bangil District. To determine the main pathogenic bacteria that cause mass mortality and the composition of microbes on rearing media infected with certain diseases and healthy ones, this study observed the water profile of ponds which include microbial composition in it using next-generation sequencing (NGS) technology. The research was conducted by taking healthy shrimp pond water samples and infected shrimp pond water samples. These results generally indicate that the disease is similar to white feces syndrome (WFS). The results of the NGS test showed that the main causative pathogen is Vibrio vulnificus because its presence in infected pond water is quite dominant.
Assessment of coral reef condition using upt (underwater photo transect) method on gili labak island madura indonesia
Authors: Irawan D , Mukti A T , Andriyono S , Muhsoni F F 
Published: December 2023

Abstract
Coral reefs are unique, complex and high-productivity marine ecosystems that make coral reefs a place to grow and breed most fish and biota in the waters, and physically the function of coral reefs is to withstand coastal abrasion, break waves and so on. To be able to manage coral reefs properly, valid data support is needed as a basis for formulating a policy. Gili Labak Island is one of the islands that is currently a tourist attraction because it has a very wide expanse of coral reefs. The UPT method is a method that utilizes technological developments, because in this method digital camera technology and computer software are used. Data retrieval in the form of underwater photos is done by shooting using an underwater digital camera. The photographs were then analyzed using computer software to obtain quantitative data. This study aims to calculate the biota in the transect. The results obtained Coral 72.92%, Dead coral (DC) 2.03, Dead Coral Algae (DCA) 9.09%, Soft Coral (SC) 0.33%, Rubble (R) 6.15%, Sand (S) 8.98%, Rock (RK) 0.52%.
Growth rate and survival rate of montipora coral transplant on faba material in Baluran National Park
Authors: Herawati E Y , Arsad S , Khasanah R I , Yudatomo  , Juniarsa E F , Ma’arif M C , Nugraha D R W , Aisyah A D , Sofarini D 
Published: December 2023

Abstract
Coral transplantation is one of the rehabilitation efforts that can be applied to accelerate the coral reef recovery process. FABA (fly ash bottom ash) is an environmentally friendly material and is declared suitable for use as a coral transplantation medium. This study aims to determine the growth rate of transplanted corals as an effort to rehabilitate damaged coral reef ecosystems and restore coral reef habitat and biota associated with coral reef communities in Bama waters, Baluran National Park, East Java. The corals studied were Montipora sp. The research location has a salinity range of around 31-34 psu, temperature 28-30°C,100% brightness, turbidity (turbidity) 1.2-1.6 NTU, current velocity between 0.07 m/s to 0.31 m/s, and sedimentation ranged from 2.81 mg/cm2/day to 5.92 mg/cm2/day, nitrate 0.004-0.026 mg/l, ammonia 0.048-0.189 mg/l, and orthophosphate 0.008-0.028 mg/l. In general, the water conditions in Bama are still in quite good condition for coral growth. During the 4 months of observation, the achievement of growth in length and height of coral species of Montipora sp. reach 60.56 mm and 21.31 mm, respectively. The growth rate of the average length and height of Montipora sp. respectively reached 15.14 mm per month and 5.33 mm per month.
Improving the quality of shellfish products with boiler-based steaming machines in Banjar Kemuning Village, Sedati, Sidoarjo
Authors: Tjahjaningsih W , Mubarak A S , Saputra E , Pursetyo K T 
Published: December 2023

Abstract
Shellfish are filter feeder animals that filter food from the mud of their habitat and are used as bioindicators of water pollution. This has an impact on food safety if it does not meet the quality standards of shellfish sanitation. To provide a sense of security to consumers is to reduce some of the hazardous materials either from biological, chemical, or physical hazards. Banjar Kemuning Village is located in Sedati District, Sidoarjo Regency, East Java has five Joint Business Groups (KUB) which are engaged in catching shellfish. Shellfish taken from the waters should be handled related to sanitation processes. One solution for processing shellfish to meet quality standards is the method of steaming shellfish based on a boiler machine. Activities include (1) counseling through face-to-face meetings and discussions, (2) demonstration plots on the application of mussel steaming technology using a steamer boiler, and (3) monitoring and evaluation of post-catch shellfish handling related to sanitation and hygiene. The application of appropriate technology with the boiler-based shellfish steaming method can increase the production and quality of shellfish products.
Crude protein and crude fiber content of fish silage fermented with kombucha tea
Authors: Pratama A W W , Satyantini W H , Kenconojati H 
Published: December 2023

Abstract
Fish silage production using mineral acid is considered to have a negative effect because it is highly corrosive. As an alternative, kombucha tea which contains several organic acids and microorganisms can be used as a starter for fish silage fermentation. This study aims to determine the effect of different doses and duration of fermentation on the crude protein and crude fiber content of fish silage. The research method used was a factorial completely randomized design (CRD) method with 2 factors, namely factor A (dose) and factor B (fermentation time). The results showed that the difference in the dose of kombucha tea was not significantly different (p>0.05) on the increase in crude protein, while the difference in fermentation time was considerably different (p<0.05). Meanwhile, the difference in kombucha tea dose was not significantly different (p>0.05) on the decrease in crude fiber, but the difference in fermentation time was significantly different (p<0.05). Based on these results, there was an interaction between the dosage and the length of fermentation time on the crude protein content, while there was no interaction in crude fiber content. In conclusion, fermentation of fish silage using kombucha tea increased crude protein content while decreasing crude fiber content.
Evaluation of larval Beautiful Groupers (Epinephelus fuscoguttatus x E. microdon) using Chlorella sp. and Artemia sp.
Authors: Ramadhany F , Sumito  , Sari L A , Nindarwi D D , Arsad S 
Published: December 2023

AbstractGrouper is one of the fishery commodities having a fairly high price and high economic value at the cultivator level. Due to the increasing market demand in the world, the larval rearing of the beautiful groupers (Epinephelus fuscoguttatusxE. microdon) has been carried out in order to obtain quality seeds. The purpose of this study was to determine the results of the evaluation of larval rearing of beautiful groupers (Epinephelus fuscoguttatusxE. microdon) usingChlorella sp. andArtemia sp. The working methods used in this study included preparation of tanks and water maintenance of culture media, management of feed, stocking and hatching of eggs, frequency and timing of feeding larvae/seeds, water quality management, pest and disease control, and grading and harvesting. Seed harvesting could be done after the seeds were aged 45 to 50 days old with a size of 3 cm. Water quality parameters measured were temperature at 28-32°C, pH at 7-8, salinity at 29-33 ppt, and DO at 6.25-7.3 mg/L. Survival rate (SR) in larval rearing was 57%, and the hatching rate (HR) was 60%.
Histopathological condition of Litopenaeus vannamei infected with Infectious Myonecrosis Virus (IMNV) on different days of culture collected from Aceh, Sidoarjo, Probolinggo and Lampung Indonesia
Authors: Puspitasari I , Suseno D N , Yudana IGP G R , Anwar M K 
Published: December 2023

Abstract
Histopathological detection is one of many methods that has been used on shrimp diseases detection. A study on histopathology of Litopenaeus vannamei infected by infectious Myonecrosis Virus (IMNV) has been conducted in Sidoarjo, East Java. The purpose of this study was to give a clear view of the histopathological condition of shrimp infected by IMNV which is necessary to examine the destructive level of the disease within the shrimp cells and hopefully can contribute to adding more information about the disease. About 16 samples of shrimp from different days of cultured that shown an IMNV infection clinically sign has been examined on their muscle tissue, lymphoid organ and hepatopancreas condition. The result has shown that shrimp infected by IMNV has a necrosis and haemocyte infiltration on muscle, spheroids on lymphoid organ and shown congestions and haemocyte infiltrations on hepatopancreas. There were significant differences (P<0.05, Kruskal-Wallis) on muscle and lymphoid organ condition between shrimp before and after 2 months cultured. But there was no significant difference in shrimp hepatopancreas condition.
Characterizing subgenome recombination and chromosomal imbalances in banana varietal lineages
Authors: Higgins Janet , Osorio-Guarín Jaime Andrés , Olave-Achury Carolina , Toloza-Moreno Deisy Lisseth , Enriquez Ayda , Di Palma Federica , Yockteng Roxana , De Vega Jose J 
Published: December 2023

Abstract

Background
Bananas and plantains (Musa spp.) are among the most important crops worldwide. The cultivated varieties are vegetatively propagated, so their genetic diversity is essentially fixed over time. Musa acuminata, M. balbisiana and M. schizocarpa have provided the named A, B and S subgenomes that predominantly constitute these varieties. Here we aimed to characterize intergenetic recombination and chromosomal imbalances between these A/B/S subgenomes, which often result in copy-number variants (CNVs) leading to changes in gene dosage and phenotype, in a diverse panel of bananas and plantains. This will allow us to characterize varietal lineages better and identify sources of genetic variation.

Methods
We delimited population structure and clonal lineages in a diverse panel of 188 banana and plantain accessions from the most common cultivars using admixture, principal component and phylogenetic analyses. We used new scalable alignment-based methods, Relative Averaged Alignment (RAA) and Relative Coverage, to infer subgenome composition (AA, AAB, etc.) and interspecific recombination.

Results
In our panel, we identified ten varietal lineages composed of somatic clones, plus three groups of tetraploid accessions. We identified chromosomal exchanges resulting in gains/losses in chromosomal segments (CNVs), particularly in AAB and ABB varieties.

Conclusions
We demonstrated alignment-based RAA and Relative Coverage can identify subgenome composition and introgressions with similar results to more complex approaches based on single nucleotide polymorphism (SNP) databases. These ab initio species-agnostic methods can be used without sequencing a panel of wild ancestors to find private SNPs, or in recently diverged pools where private SNPs are uncommon. The extensive A/B/S exchanges and the variation in the length of some introgressions between lineages further support multiple foundational events of hybridization and residual backcrossing. Imbalances between A/B/S may have resulted in CNVs and gene dosage variation. Since most edible banana genomes are fixed on time, these CNVs are stable genetic variations probably associated with phenotypic variation for future genetic studies.
Anti-seizure gene therapy for focal cortical dysplasia
Authors: Almacellas Barbanoj Amanda , Graham Robert T , Maffei Benito , Carpenter Jenna C , Leite Marco , Hoke Justin , Hardjo Felisia , Scott-Solache James , Chimonides Christos , Schorge Stephanie , Kullmann Dimitri M , Magloire Vincent , Lignani Gabriele 
Published: December 2023

Abstract
Focal cortical dysplasias are a common subtype of malformation of cortical development, which frequently presents with a spectrum of cognitive and behavioural abnormalities as well as pharmacoresistant epilepsy. Focal cortical dysplasia type II is typically caused by somatic mutations resulting in mammalian target of rapamycin (mTOR) hyperactivity, and is the commonest pathology found in children undergoing epilepsy surgery. However, surgical resection does not always result in seizure freedom, and is often precluded by proximity to eloquent brain regions. Gene therapy is a promising potential alternative treatment and may be appropriate in cases that represent an unacceptable surgical risk. Here, we evaluated a gene therapy based on overexpression of the Kv1.1 potassium channel in a mouse model of frontal lobe focal cortical dysplasia. An engineered potassium channel (EKC) transgene was placed under control of a human promoter that biases expression towards principal neurons (CAMK2A) and packaged in an adeno-associated viral vector (AAV9). We used an established focal cortical dysplasia model generated by in utero electroporation of frontal lobe neural progenitors with a constitutively active human Ras homolog enriched in brain (RHEB) plasmid, an activator of mTOR complex 1. We characterized the model by quantifying electrocorticographic and behavioural abnormalities, both in mice developing spontaneous generalized seizures and in mice only exhibiting interictal discharges. Injection of AAV9-CAMK2A-EKC in the dysplastic region resulted in a robust decrease (∼64%) in the frequency of seizures. Despite the robust anti-epileptic effect of the treatment, there was neither an improvement nor a worsening of performance in behavioural tests sensitive to frontal lobe function. AAV9-CAMK2A-EKC had no effect on interictal discharges or behaviour in mice without generalized seizures. AAV9-CAMK2A-EKC gene therapy is a promising therapy with translational potential to treat the epileptic phenotype of mTOR-related malformations of cortical development. Cognitive and behavioural co-morbidities may, however, resist an intervention aimed at reducing circuit excitability.
Exploring the role of mutual prediction in inter-brain synchronization during competitive interactions: an fNIRS hyperscanning investigation
Authors: Zhang Yuxuan , Ye Weihao , Yin Junting , Wu Qin , Huang Yao , Hao Na , Cui Liying , Zhang Mingming , Cai Dan 
Published: December 2023

Abstract
Mutual prediction is crucial for understanding the mediation of bodily actions in social interactions. Despite this importance, limited studies have investigated neurobehavioral patterns under the mutual prediction hypothesis in natural competitive scenarios. To address this gap, our study employed functional near-infrared spectroscopy hyperscanning to examine the dynamics of real-time rock-paper-scissors games using a computerized paradigm with 54 participants. Firstly, our results revealed activations in the right inferior frontal gyrus, bilateral dorsolateral prefrontal cortex, and bilateral frontopolar cortex, each displaying distinct temporal profiles indicative of diverse cognitive processes during the task. Subsequently, a task-related increase in inter-brain synchrony was explicitly identified in the right dorsolateral prefrontal cortex, which supported the mutual prediction hypothesis across the two brains. Moreover, our investigation uncovered a close association between the coherence value in the right dorsolateral prefrontal cortex and the dynamic predictive performances of dyads using inter-subject representational similarity analysis. Finally, heightened inter-brain synchrony values were observed in the right dorsolateral prefrontal cortex before a draw compared to a no-draw scenario in the second block, suggesting that cross-brain signal patterns could be reflected in behavioral responses during competition. In summary, these findings provided initial support for expanding the understanding of cognitive processes underpinning natural competitive engagements.
Using artificial intelligence to promote equitable care for inpatients with language barriers and complex medical needs: clinical stakeholder perspectives
Authors: Barwise Amelia K , Curtis Susan , Diedrich Daniel A , Pickering Brian W 
Published: December 2023

Abstract

Objectives
Inpatients with language barriers and complex medical needs suffer disparities in quality of care, safety, and health outcomes. Although in-person interpreters are particularly beneficial for these patients, they are underused. We plan to use machine learning predictive analytics to reliably identify patients with language barriers and complex medical needs to prioritize them for in-person interpreters.

Materials and methods
This qualitative study used stakeholder engagement through semi-structured interviews to understand the perceived risks and benefits of artificial intelligence (AI) in this domain. Stakeholders included clinicians, interpreters, and personnel involved in caring for these patients or for organizing interpreters. Data were coded and analyzed using NVIVO software.

Results
We completed 49 interviews. Key perceived risks included concerns about transparency, accuracy, redundancy, privacy, perceived stigmatization among patients, alert fatigue, and supply–demand issues. Key perceived benefits included increased awareness of in-person interpreters, improved standard of care and prioritization for interpreter utilization; a streamlined process for accessing interpreters, empowered clinicians, and potential to overcome clinician bias.

Discussion
This is the first study that elicits stakeholder perspectives on the use of AI with the goal of improved clinical care for patients with language barriers. Perceived benefits and risks related to the use of AI in this domain, overlapped with known hazards and values of AI but some benefits were unique for addressing challenges with providing interpreter services to patients with language barriers.

Conclusion
Artificial intelligence to identify and prioritize patients for interpreter services has the potential to improve standard of care and address healthcare disparities among patients with language barriers.
Lifestyle factors associated with presenteeism among city government office workers: a cross-sectional study
Authors: Otsubo Tsubasa , Kinjo Aya , Kuwabara Yuki , Hongja Kim , Osaki Yoneatsu 
Published: December 2023

Abstract
Objectives: Presenteeism is a critical issue in occupational health. This study aimed to examine the association between presenteeism and subjective sleep quality, smoking status, and alcohol consumption.
Methods: Anonymous data of 777 workers in a Japanese city were retrospectively obtained from City Government Office A. They included variables like absolute presenteeism scores (measured using the Japanese version of the World Health Organization Health and Work Performance Questionnaire short form), gender, age, family status, subjective sleep quality, smoking status, and alcohol consumption. A binary logistic regression analysis was performed with gender, age, family status, subjective sleep quality, smoking status, and alcohol consumption as the independent variables, and absolute presenteeism scores equal to or below 40 as the dependent variable. A gender-stratified binary logistic regression analysis was also performed.
Results: The logistic regression analysis results revealed that absolute presenteeism was positively associated with poor subjective sleep quality among all respondents (odds ratio [OR], 1.70; 95% CI, 1.18-2.44) and men (OR, 1.85; 95% CI, 1.12-3.05) and with current drinkers among women (OR, 3.49; 95% CI, 1.36-8.92); it was negatively associated with age among those who were ≥50 years old (OR, 0.50; 95% CI, 0.27-0.93) and with current drinkers among men (OR, 0.43; 95% CI, 0.20-0.92).
Conclusions: The factors associated with presenteeism differed between men and women office workers, suggesting that gender differences need to be considered when working toward improving workers’ productivity.