bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluctuations in Detection Indicators and Their Significance for the Diagnosis of Early Pressure Injury in Rat Models
Authors: Hou Ping , Chen Lu , Takashi En , Kamijo Akio , Xu Huiwen , Miura Daiji , Ten Hirotomo , Kitayama Akio , Fan Jianglin 
Published: January 2024

PURPOSE:
The aim of this study was to identify the most meaningful diagnostic indicator for distinguishing blanchable erythema (BE) and stage 1 pressure injury (early PI) in an in vivo (rat) model.

DESIGN:
A prospective case-control design was used to complete a horizontal and vertical comparison of detection indicators during the process of fading of BE or the deterioration of early PI into ulcer in rat models.

MATERIALS AND SETTING:
The sample comprised 5 hairless rats with 20 injuries, of which 10 were BE and the other 10 were early PI. Data were collected at Nagano College of Nursing in 2020 in Nagano, Japan.

METHODS:
The BE and PI rat models were established by subjecting the dorsal skin of a hairless rat to compression between 2 neodymium magnets for 45 minutes and 3.45 hours, respectively. The affected skin was observed based on the following: (1) photography, (2) hardness, (3) temperature, (4) moisture, and (5) spectrophotometric (a* value and ultraviolet [UV] reflectance) measurements. All measurements of BE were performed at the beginning to 60 minutes after decompression, and those for early PI were performed until 48 hours after decompression.

RESULTS:
Multiple BE factors, such as the degree of erythema (macroscopy and a* value), hardness, temperature, and moisture, were found to have unstable fluctuations. Only UV reflectance gradually decreased from 6 hours and decreased significantly at 48 hours after decompression (P = .001 vs 1 hour). In contrast to early PI, erythema in BE obviously faded within 10 minutes.

CONCLUSIONS:
Study findings indicate that a continuous decrease in UV reflectance can reflect the worsening of hemorrhage in early (stage 1) PI. In contrast, other indicators including photography, skin hardness, temperature, and moisture fluctuated and did not prove predictive for PI progression. The obvious fading of erythema in BE a short time after decompression can be used for clinical observations.
Caring for Patients With Malignant Fungating Wounds
Authors: Yasmara Deni , Tam Sin-Hang , Fang Su-Ying 
Published: January 2024

PURPOSE:
The aim of this scoping literature review was to identify current knowledge related to management of malignant fungating wounds (MFWs), gaps in this knowledge, and areas for future research.

METHODS:
This was a scoping literature review.

SEARCH STRATEGY:
Six electronic databases (Embase, MEDLINE, Cochrane (CENTRAL), CINAHL, Scopus, and Web of Science) were searched spanning the years 1987 and 2022. The initial electronic database search retrieved 6041 studies. The search is based on 60 articles published between 1987 and 2022, along with 14 additional studies were based on search of reference lists of key articles.

FINDINGS:
Studies were divided into 2 main domains; the vast majority (65 of 72, 87.8%) focused on symptom impact, measurement, and management. The remaining articles (n = 9, 12.2%) focused on caring for patients with MFW; these studies focused on nursing care experiences and competencies, along with lay caregiver experiences. Findings indicated that instruments have been developed to evaluate symptoms of MFW but only a few have been validated. Most of the studies reviewed evaluated the influence of MFW on physical and psychosocial function. Local and systematic symptom managements such as modern dressings, wound care strategies, surgery, and radiotherapy as well as chemotherapy were found to be effective in alleviating symptoms. Findings also indicated that families and nurses encounter obstacles and burdens when caring for patients with MFW; limited studies were retrieved that investigated the competence and needs of nurses in their caring journey.

IMPLICATIONS:
Future studies should focus on: (1) using better designs to evaluate and compare topical agents and dressings to control odor, (2) validating assessment tools specific to MFW, (3) qualitative studies designed to enhance understanding family and nurses' experiences, and (4) improving the nurse's ability to care for patients with MFWs and their responses to managing these challenging patients.
Health Inequity in Likelihood and Time to Renal Recovery after Living Kidney Donation: Implications for Kidney Health in Black Americans
Authors: Rabbani Muhammad Umaid , Reed Rhiannon D , McLeod Marshall C , MacLennan Paul A , Kumar Vineeta , Locke Jayme E 
Published: January 2024

BACKGROUND:
Live donor kidney transplantation has been popularized to help mitigate the organ shortage crisis. At the time of living donor nephrectomy, living donors lose 50% of their kidney function or glomerular filtration rate (GFR). Studies have shown that in healthy living donors, the remaining kidney is able to adapt and recover 10% to 25% of postdonation lost GFR. GFR recovery is critical to long-term kidney health, particularly for Black Americans who disproportionately suffer from kidney disease with an incidence 2.5 times White Americans. To date, no study has examined whether health inequities in renal recovery postdonation exist.

STUDY DESIGN:
We retrospectively analyzed 100,121 living kidney donors reported to the Scientific Registry of Transplant Recipients between 1999 and 2021. We estimated GFR (eGFR) using the Chronic Kidney Disease Epidemiology Collaboration 2021 equation and predicted the likelihood (logistic regression) and time (Cox regression) to recovery of 60% and 75% predonation eGFR. Models adjusted for age, sex, race, BMI, and predonation eGFR.

RESULTS:
Black patients were 47% (adjusted odds ratio 0.53, 95% CI 0.50 to 0.56, p < 0.001) and 43% (adjusted odds ratio 0.57, 95% CI 0.54 to 0.60, p < 0.001) less likely to recover 60% and 75% of predonation eGFR, respectively, compared with their White counterparts. The hazard ratio for time to renal recovery of 60% and 75% of predonation eGFR was 22% (adjusted hazard ratio 0.78, 95% CI 0.76 to 0.80, p < 0.001) and 38% (adjusted hazard ratio 0.62, 95% CI 0.60 to 0.65, p < 0.001) lower, respectively, than White patients.

CONCLUSIONS:
Black living kidney donors were less likely to recover predonation eGFR, and time to renal recovery was significantly longer than their White counterparts. These data highlight the need for enhanced living kidney donor follow-up, particularly for Black living kidney donors who are at greatest future risk of end-stage kidney disease.
The interplay of facilitation and competition drives the emergence of multistability in dryland plant communities
Authors: Pichon Benôıt , Gounand Isabelle , Donnet Sophie , Kéfi Sonia 
Published: January 2024

AbstractSpecies are wrapped in a set of feedbacks within communities and with their abiotic environment, which can can generate alternative stable states. So far, research on alternative stable states has mostly focused on systems with a small number of species and a limited diversity of interaction types. Here, we analyze a spatial model of plant community dynamics in drylands, where each species is characterized by a strategy, and interact through facilitation and competition. Our work identifies three different types of multistability emerging from the interplay of competition and facilitation. Under low-stress levels, the community organizes in small groups of coexisting species maintained by space and facilitation, while under higher stress levels, positive feedbacks from competition and facilitation lead to the dominance of a single species before desertification happens. Our study paves the way for bridging community ecology and alternative states theory in a common framework.
The medial entorhinal cortex encodes multisensory spatial information
Authors: Nguyen Duc , Wang Garret , Gu Yi 
Published: January 2024

AbstractAnimals employ spatial information in multisensory modalities to navigate their natural environments. However, it is unclear whether the brain encodes such information in separate cognitive maps or integrates all into a single, universal map. We addressed this question in the microcircuit of the medial entorhinal cortex (MEC), a cognitive map of space. Using cellular-resolution calcium imaging, we examined the MEC of mice navigating virtual reality tracks, where visual and auditory cues provided comparable spatial information. We uncovered two cell types: “unimodality cells” and “multimodality cells”. The unimodality cells specifically represent either auditory or visual spatial information. They are anatomically intermingled and maintain sensory preferences across multiple tracks and behavioral states. The multimodality cells respond to both sensory modalities with their responses shaped differentially by auditory and visual information. Thus, the MEC enables accurate spatial encoding during multisensory navigation by computing spatial information in different sensory modalities and generating distinct maps.
The 22q11.2 deletion syndrome from a biopsychosocial perspective: an ICF-based approach
Authors: Corrêa Cabral Ana Paula , Gandelman Horovitz Dafne Dain , Santos Lidiane Nogueira , de Carvalho Carvalho Amanda Oliveira , Wing Cristina Maria Duarte , Castaneda Luciana , Simon Liane , Ribeiro Carla Trevisan M. 
Published: January 2024

AbstractPurposeTo describe children with 22q11.2 deletion syndrome functioning from a biopsychosocial perspective, focusing on the impact of children’s health condition from domains to the International Classification of Functioning, Disability and Health (ICF).MethodsA descriptive, cross-sectional case series study with 22q11.2del children. A questionnaire with an ICF checklist for 22q11.2del was completed using a structured interview. The Test of Childhood Language ABFW was used to fill in vocabulary, fluency and language pragmatics questions. The Wechsler Abbreviated Scale of Intelligence (WASI) was used to determine Intelligence Quotient (IQ).ResultsSeven participants from 7 to 12 years old, presented some level of IQ impairment. Observed that 22q11.2del children experience significant intellectual, cognitive and speech impairment across ICF Body Function domains. Impairment related to nose and pharynx were found in only one patient. The most relevant categories considered limitations in Activity and Participation component were pertained to producing nonverbal messages, communication, handling stress and social interaction. Family, health professionals and acquaintances were perceived as facilitators in the component Environmental Factors.ConclusionChildren with 22q11.2del have their functioning affected by aspects that go beyond medical diagnosis. A checklist base on ICF for functional profile can contribute to incorporate a biopsychosocial approach.
Anxiety appraisal in mothers of preterm neonates admitted in critical care unit
Authors: Gul Rafia , Fatima Samer , Khurshid Samina , Niamat Sidra , Anwar Zahid , Ahdi Saher Gul 
Published: January 2024

AbstractBackgroundMothers of premature neonates often confront various psychological challenges including postpartum depression, anxiety, and elevated stress levels. However, anxiety has not received the necessary emphasis in routine clinical practice and research, often going unnoticed. There is insufficient data regarding the utilization of specific tools for screening maternal anxiety in hospitalized preterm infants. The study aimed to determine the prevalence of maternal anxiety and its risk factors among mothers of premature neonates admitted to the intensive care unit.MethodsFollowing Institutional Review Board (IRB) approval, a descriptive cross-sectional study was conducted at level-III Neonatal Unit, Fatima Memorial Hospital Shadman, Lahore over duration of 30 months, (January 2021-July 2023). Mothers werescreened for anxiety using Perinatal Anxiety Screening Scale (PASS) having 31 items. Comparative descriptive statistics and multiple logistic regression were applied to identify all risk factor while taking p <0.05 as significant.ResultsOut of 430 mothers, 28.1% experienced anxiety. Statistically significant factors for maternal anxiety include young age, urban residence, higher education, smoking, diabetes, depression/anxiety diagnosis, primiparity, delivery complications, maternal involvement in neonatal care, breastfeeding, fear of handling preterm infants at home, and prematurity-related concerns, as well as gestational age, birth weight, and weight for gestational age (p < 0.05). Among all these, however, prematurity <28 weeks (p < 0.001, AOR 496, 95% CI 22.5–10951) and 28-31+6 weeks (p < 0.001, AOR 265, 95% CI 14– 5010), alongwith primiparity (p = 0.001, AOR 16.483, 95% CI 3.287–82.648), and SGA (p = 0.025, AOR 8.9, 95% CI 1.3–60.6) increase while extended family system protects from maternal anxiety (p = 0.002, AOR 0.25, 95% CI 0.106–0.595).ConclusionEvery third mother in our study population who delivered prematurely experienced anxiety. The younger gestational age, first-time motherhood, SGA neonate increase while the extended family system serves as a protective factor against maternal anxiety.
Annexin- and calcium-regulated priming of legume root cells for endosymbiotic infection
Authors: Guillory Ambre , Fournier Joëlle , Kelner Audrey , Hobecker Karen , Auriac Marie-Christine , Frances Lisa , Delers Anaïs , Pedinotti Léa , Ru Aurélie Le , Keller Jean , Delaux Pierre-Marc , Gutjahr Caroline , Frei Dit Frey Nicolas , de Carvalho-Niebel Fernanda 
Published: January 2024

AbstractLegumes establish endosymbioses with arbuscular mycorrhizal (AM) fungi or rhizobia bacteria to improve mineral nutrition. Symbionts are hosted in privileged habitats, root cortex (for AM fungi) or nodules (for rhizobia) for efficient nutrient exchange. To reach these habitats, plants form cytoplasmic bridges, which are key to predicting and guiding the cellular route of entry of fungal hyphae or rhizobia-filled infection threads (ITs). However, the underlying mechanisms are poorly studied. Here we show that unique ultrastructural changes and Ca2+spiking signatures, closely linked to MtAnn1 annexin accumulation, accompany rhizobia-associated bridge formation. Loss ofMtAnn1function inM. truncatulaaffects Ca2+spike amplitude, cytoplasmic configuration and rhizobia infection efficiency, consistent with a role of MtAnn1 in regulating infection priming.MtAnn1,which evolved in species establishing intracellular symbioses, is also AM-symbiosis-induced and required for proper arbuscule formation. Together, we propose that MtAnn1 is part of an ancient Ca2+-regulatory module for transcellular endosymbiotic infection.
Rgf1 GEF activity toward Rho1 defines a new actin-dependent signal to determine growth sites independently of microtubules and Tea1
Authors: Garcia Patricia , Celador Ruben , Edreira Tomas , Sanchez Yolanda 
Published: January 2024

ABSTRACTCellular asymmetry begins with the selection of a discrete point on the cell surface that triggers Rho-GTPases activation and localized assembly of the cytoskeleton to establish new growth zones. The cylindrical shape of fission yeast is organized by microtubules that deliver the landmark Tea1–Tea4 complex at the cell tips to define the growth poles. However, only a fewtea1Δ cells mistaken the direction of growth, indicating that they manage to detect their growth sites. Here we show that Rgf1 (Rho1-GEF) and Tea4 are components of the same complex and that Rgf1 activity toward Rho1 is required for strengthen Tea4 at the cell tips. Moreover, in cells lacking Tea1, selection of the correct growth site depends on Rgf1 and on a correctly polarized actin cytoskeleton, both necessary for Rho1 activation at the pole. We propose an actin-dependent mechanism driven by Rgf1–Rho1 that marks the poles independently of microtubules and the Tea1–Tea4 complex.
Mechanism and regulation of cargo entry into the Commander recycling pathway
Authors: Butkovič Rebeka , Walker Alexander P. , Healy Michael D. , McNally Kerrie E. , Liu Meihan , Kato Kohji , Collins Brett M. , Cullen Peter J. 
Published: January 2024

ABSTRACTCommander is a multiprotein complex that orchestrates endosomal recycling of diverse integral cargo proteins and in humans is required for normal skeletal, brain, kidney, and cardiovascular development. While the structure of this complex has recently been described, the central question of how cargo proteins are selected for entry into the Commander recycling pathway remains unclear. Here using recombinant protein reconstitution andin silicopredictions we identify the evolutionary conserved mechanism through which the unstructured carboxy-terminal tail of the integral protein adaptor sorting nexin-17 (SNX17) directly binds to the Retriever sub-complex of Commander. SNX17 adopts an autoinhibited conformation where its carboxy-terminal tail occupies the cargo binding groove. Competitive cargo binding overcomes this autoinhibition, promoting SNX17 endosomal residency and the release of the carboxy tail for Retriever association. Using molecular cell biology and high-resolution microscopy, we establish the central importance of SNX17-Retriever association in the handover of integrin and lipoprotein receptor cargoes into pre-existing endosomal retrieval sub-domains for entry into the recycling pathway. In describing the principal mechanism of cargo entry into the Commander recycling pathway we provide key insight into the function and regulation of this evolutionary conserved sorting complex.
Growth phase-dependent reorganization of cryptophyte photosystem I antennae
Authors: Zhang Shumeng , Si Long , Su Xiaodong , Li Mei 
Published: January 2024

AbstractPhotosynthetic cryptophytes are eukaryotic algae that utilize membrane-embedded chlorophyll a/c binding proteins (CACs) and lumen-localized phycobiliproteins (PBPs) as their light-harvesting antennae. Cryptophytes go through logarithmic and stationary growth phases, and may adjust their light-harvesting capability according to their particular growth state. How cryptophytes change the type/arrangement of the photosynthetic antenna proteins to regulate their light-harvesting remains unknown. Here we solved four structures of cryptophyte photosystem I (PSI) bound with CACs that show the rearrangement of CACs at different growth phases. We identified a cryptophyte-unique protein, PsaQ, which harbors three chlorophyll molecules. PsaQ specifically binds to the lumenal region of PSI during logarithmic growth phase and may assist the association of PBPs with photosystems and energy transfer from PBPs to photosystems.
High-throughput formulation of reproducible 3D cancer microenvironments for drug testing in myelogenous leukemia
Authors: Rudzinska-Radecka M. , Turos-Korgul L. , Mukherjee D. , Podszywalow-Bartnicka P. , Piwocka K. , Guzowski J. 
Published: January 2024

AbstractTargeting cancer microenvironment is currently one of the major directions in drug development and preclinical studies in leukemia. Despite the variety of available chronic myelogenous leukemia 3D culture models, the reproducible generation of miniaturized leukemia microenvironments, suitable for high-throughput drug testing, has remained a challenge. Here, we use microfluidics to generate over ten thousand highly monodisperse leukemic-bone marrow hydrogel microbeads per minute. We employ gelatin methacrylate (GelMA) as a model extracellular matrix (ECM) and tune the concentration of the biopolymer, as well as other possible components of the ECM (fibrin, hyaluronic acid), cell concentration and the ratio of leukemic cells to bone marrow cells within the microbeads. This allows to achieve optimal cell viability and the propensity of the encapsulated cells to microtissue formation, while also warranting long-term stability of the microbeads in culture. We administer model kinase inhibitor, imatinib, at various concentrations to the microbeads and, via comparing mono-and co-culture conditions (cancer alone vs cancer-stroma), we find that the stroma-leukemia crosstalk systematically protects the encapsulated cells against the drug-induced cytotoxicity, confirming therefore that our system mimics the physiological stroma-dependent protection. We finally discuss applicability of our model to (i) studying the role of direct-or close-contact interactions between leukemia and bone marrow cells embedded in 3D ECM on the stroma-mediated protection, and (ii) high-throughput screening of anti-cancer therapeutics in personalized therapies.
Meta-metabolome ecology reveals that geochemistry and microbial functional potential are linked to organic matter development across seven rivers
Authors: Danczak Robert E. , Goldman Amy E. , Borton Mikayla A. , Chu Rosalie K. , Toyoda Jason G. , Garayburu-Caruso Vanessa A. , Graham Emily B. , Morad Joseph W. , Renteria Lupita , Hager Jacqueline R. , Arnon Shai , Brooks Scott , Bar-Zeev Edo , Jones Michael , Jones Nikki , Lewandowski Jorg , Meile Christof , Mueller Birgit M. , Schalles John , Schulz Hanna , Ward Adam , Stegen James C. 
Published: January 2024

AbstractRivers receive substantial dissolved organic matter (DOM) input from the land and transport it to the ocean. As DOM travels through watersheds, it undergoes biotic and abiotic transformations that impact biogeochemical cycles and any subsequent CO2release into the atmosphere. While recent research has increased our mechanistic knowledge of DOM composition within watersheds, DOM development across broad spatial distances and within divergent biomes is under investigated. Here, we combined DOM characterization, geochemical analyses, and shotgun metagenomics to analyze samples from seven rivers ranging from the U.S. Pacific Northwest to Berlin, Germany. Initial analyses revealed that many DOM properties were distinguished by river type (e.g., wastewater, headwater) and that geochemistry often explained variation across rivers. At a global scale, analyses rooted in meta-metabolome ecology indicated that DOM was structured overwhelmingly by deterministic selection. When controlling for scale, however, analyses indicated that ecological assembly dynamics were again partially structured by river type. Finally, microbial analyses revealed that many riverine microbes from our systems shared core metabolic functional potential while differing in peripheral capabilities in across the rivers. Further analysis of the carbon degradation potential for recovered metagenomically assembled genomes indicated that the sampled rivers had strong taxonomically conserved niche differentiation and that carbon degradation potential diversity was significantly related to organic matter diversity. Together, these results help us uncover interconnections between the development of DOM, riverine geochemistry, and microbial functional potential.