bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMMEDIATE AND LONG-TERM RESULTS OF MINIMALLY INVASIVE AORTIC VALVE REPLACEMENT: SINGLE CENTER RETROSPECTIVE STUDY
Authors: Enginoev Soslan T. , Zenkov Alexander A. , Magomedov Hasan M. , Abdulmejidova Uzlipat K. , Chertoev Shamil H. , Chernov Igor I. 
Published: January 2024

HighlightsWe have conducted a retrospective analysis of patients who underwent minimally invasive aortic valve replacement, and evaluated immediate and long-term results of the surgery. AbstractAim. To study the immediate and long-term results of minimally invasive aortic valve (AV) replacement.Methods. The retrospective study included 208 patients with AV stenosis undergoing minimally invasive aortic valve replacement in the period from 2010 to 2019. The ratio of men to women was 1:1. The mean age of the patients was 63 (58–68) years old, with the minimum age being 29 years and the maximum being 81 years. The median follow-up period was 45.5 (11–68) months. Endpoints were as follows: hospital mortality, postoperative complications, long-term outcome (long-term survival, freedom from reoperation). Inclusion criteria were as follows: patients aged 18 years and older undergoing minimally invasive aortic valve replacement. Exclusion criteria were as follows: AVNeo, Ross procedure and AV replacement with aortic homograft, combined interventions (except for intervention on the ascending aorta), and standard access site.Results. The majority of patients (199 (95.7%) underwent “J”-shaped ministernotomy through the III-IV intercostal space. As a part of the combined intervention, ascending aorta replacement was performed in 6 (2.9%) patients. The duration of cardiopulmonary bypass and aortic cross-clamping was 91 (78–106) min and 68 (60–78) min, respectively. Due to conduction disorders, implantation of a pacemaker was performed in 5 (2.4%) patients, stroke was diagnosed in 1 (0.5%) patient, resternotomy for bleeding was performed in 4 (1.9%) patients, cardiac tamponade in 2 (1 %) patients, sternal infection was found in 1 (0.5%) patient. There were no complications such as AKI, perioperative MI, or hospital mortality in any patient. Five- and ten-year survival after surgical treatment of patients was 88.2% and 80.5%, respectively. Five- and ten-year freedom from reoperation on AV was 96.2% and 96.2%.Conclusions. Minimally invasive AV replacement is a measurable procedure with negligible complication rates. Minimally invasive aortic valve replacement has shown its effectiveness in the long-term period with acceptable rates of survival and freedom from reoperation.
RISK FACTORS FOR ATRIAL TACHYARRHYTHMIA RECURRENCE IN PATIENTS WITH NON-PAROXYSMAL ATRIAL FIBRILLATION AFTER «BOX LESION SET» THORACOSCOPIC ABLATION
Authors: Kadyrova Madina , Strebkova Elizaveta D. , Yalova Evgeniya V. , Karmazanovsky Grigory G. , Revishvili Amiran S. 
Published: January 2024

Highlights Identification of risk factors impacting the effectiveness of thoracoscopic ablation according to the box session set scheme will allow us to formulate criteria for the selection of patients with non-paroxysmal atrial fibrillation for epicardial ablation of the left atrium.Duration of atrial fibrillation for more than 7.5 years and the volume of the left atrium indexed to the body surface area of more than 47 mL/m2 statistically significantly reduce the effectiveness of thoracoscopic ablation of stable atrial fibrillation by 34.2 and 55.8% during the three-year follow-up period, respectively.The need to assess the function of left atrium using modern two-dimensional speckle-tracking echocardiography technology should be considered to determine the criteria for the successful ablation of non-paroxysmal atrial fibrillation. The aim. To study risk factors for atrial tachyarrhythmias recurrence after thoracoscopic ablation (TSA) of non-paroxysmal atrial fibrillation (AF) in the long-term follow-up period.Methods. The retrospective study included a series of 100 patients with non-paroxysmal AF undergoing “Box lesion set” TSA with one-stage exclusion of the left atrial (LA) appendage. The efficacy of TSA in the long-term follow-up period was evaluated using 24-h HM ECG results. A retrospective analysis of risk factors for atrial tachyarrhythmias recurrence after TSA was performed. The analysis included the main clinical characteristics of patients in the preoperative period, as well as data of transthoracic echocardiography with inclusion of left atrial strain in the reservoir and conduit phases.Results. In the presented study, the main risk factors for atrial tachyarrhythmias recurrence after TSA were: duration of AF more than 7.5 years and LAVI more than 47 mL/m2. However, univariate analysis showed that for every 1-year increase in the duration of AF, the risk of arrhythmia recurrence after TSA increased 1.07-fold [95% CI: 1.01; 1.17, p = 0.0374], and for every 10 mL/m2 increase in LAVI, the risk of atrial tachyarrhythmia recurrence increased 1.95-fold [95% CI: 1.49; 2.56, p<0.001]. According to the data of two-dimensional speckle-tracking echocardiography there was a tendency towards the decrease of the parameters of left atrial strain in the reservoir phase mainly in patients with AF, which requires further more detailed study on a larger cohort of patients.Conclusion. It is necessary to consider not only the assessment of the anatomy of the left atrium, but its function with the use of modern methods of radial imaging at the preoperative stage in order to improve the effectiveness of TSA “Box lesion set” for non-paroxysmal AF in the long-term follow-up period.
Mycobacterial infections: features of microbiological diagnosis
Authors: Lutsenko A. V. , Yasenyavskaya A. L. , Samotrueva M. A. 
Published: January 2024

To date, more than 200 species of mycobacteria have been identified, in addition to the well-known Mycobacterium leprae and Mycobacterium tuberculosis. Among microorganisms belonging to the genus Mycobacterium, there are obligate pathogenic, opportunistic and saprophytic strains. The incidence of non-tuberculous or atypical mycobacteria, which cause opportunistic infections in humans and animals, is steadily increasing. Non-tuberculous mycobacteria are increasingly recognized as a source of healthcare-associated infections.Aim of the study was to analyze the literature on current methods of microbiological diagnosis of mycobacterial infections.Material and methods. A search and analysis of scientific literature in the Web of Science, PubMed, eLIBRARY.RU, Europe PMC databases was performed using the following key words: mycobacteriosis, non-tuberculous mycobacteria, mycobacterial infections, MALDITOF MS, atypical mycobacteria. Results and discussion. The review summarizes and presents the classification, morphological, cultural, genetic and ecological features of mycobacterial strains. Modern approaches in the diagnosis of mycobacterial diseases and identification of pathogens are analyzed; their advantages and disadvantages are indicated.Conclusions. Mycobacterial infections are often considered as diseases associated with the provision of medical care, requiring a detailed assessment of the situation with the definition of criteria for microbiological monitoring of objects of a medical organization, etc. The analyzed literature data demonstrate a variety of methods for laboratory diagnosis of mycobacterial infections with the need for further improvement of methodological approaches.
Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy
Authors: Abdrakhmanova R. O. , Tazova E. G. , Genatullina G. N. , Baeva G. R. , Daudova A. D. , Demina Yu. Z. , Yasenyavskaya A. L. , Rubalsky O. V. 
Published: January 2024

Staphylococcus aureus has a high level of antibiotic resistance and is a common cause of nosocomial infections, which primarily requires development of new therapeutic strategies.Aim of the study was to investigate the level and spectrum of antibiotic resistance of S. aureus isolates for further isolation of virulent bacteriophages.Material and methods. The study used samples of clinical material obtained on the basis of the City Clinical Hospital No. 3 named after S.M. Kirov (Astrakhan). Bacterial strains isolated from patients were identified on the basis of morphological, cultural, biochemical and molecular genetic characteristics. The determination of the resistance of the isolated cultures was carried out by the disco-diffusion method using standardized commercial discs with antibiotics, in accordance with the guidelines.Results. Evaluation of antibiotic resistance of Staphylococcus aureus isolated during the study of clinical material from patients and analysis of sensitivity level of isolated strains of S. aureus allow us to conclude that all S. aureus isolated from patients are resistant to at least one antibiotic, and most strains are characterized by multidrug resistance to antibiotics.Conclusions. The presence of multi-resistant strains indicates the need to search for new approaches in the treatment of staphylococcal infection and to develop effective means for combination therapy based on virulent staphylococcal bacteriophages.
Complementary and Alternative Therapy for Picky Eater: Systematic Review
Authors: Muflih Muflih , Widaryanti Rahayu , Indrawati Fika Lilik , Trisagita Natasya Gabryella 
Published: January 2024

The picky eater problem is one of the beginnings of toddler stunting. This problem needs to be solved with scientifically proven interven- tions. This systematic review is focused on looking at complementary and alternative interventions that are scientifically proven to be ef- fective in treating picky eaters. This study aims to identify, evaluate, and summarize the findings of all relevant literature on complemen- tary & alternative therapies for picky eaters in children (1-6 years). Search for articles on PubMed, ProQUestProQuest, Google Scholar, and Crossref search engines related to literature from 2012-2022. Inclusion criteria were original research articles that examined an intervention on picky eating in children with complementary & alter- native therapeutic approaches. Quantitative, experimental research published in English and Indonesian with child participants with picky eater problems. The data were studied and analyzed with qualitative and narrative methods. There are a few articles (10 items) that re- view the effectiveness of an intervention to treat nutritional problems and the symptoms of picky eaters. Interventions with complementary approaches and alternative therapies were found to be very few. Most of them are in the form of conventional interventions such as health education, oral supplementation, counselling, and interventions with similar approaches. Several interventions are given in combination. There is an intervention for handling picky eaters given to respon- dents who have nutritional problems accompanied by other diseases. There is an insufficient number of scientific articles found related to interventions for picky eaters with complementary approaches and alternative therapies, therefore scientific evidence with research and publications is still a big opportunity and is very much needed in the future.
Comparative effects of canagliflozin and sitagliptin in chronically ischemic myocardium
Authors: Sabe Sharif A. , Harris Dwight D. , Broadwin Mark , Xu Cynthia M. , Sabra Mohamed , Banerjee Debolina , Abid M. Ruhul , Sellke Frank W. 
Published: January 2024

Aim: Recent studies demonstrate that sodium-glucose cotransporter 2 inhibitors (SGLT2i) and dipeptidyl peptidase-4 inhibitors (DPP4i), two classes of antidiabetic drugs, are cardioprotective. However, the mechanisms of these benefits and their comparative efficacy remain unclear. We aimed to compare the effects of these antidiabetic agents on cardiac function, perfusion, and microvascular density using a swine model of chronic myocardial ischemia.
Methods: Chronic myocardial ischemia was induced in Yorkshire swine by ameroid constrictor placement to the left circumflex artery. Two weeks later, pigs were administered vehicle (“CON”, 8 pigs), 300 mg SGLT2i canagliflozin, (“CANA”, 8 pigs), or 100 mg DPP4i sitagliptin (“SIT”, 5 pigs) daily. Five weeks later, pigs were euthanized. Cardiac function, perfusion, collateralization, and protein expression were determined by pressure-volume catheter, microsphere analysis, immunofluorescence, and immunoblotting, respectively.
Results: Compared with SIT, CANA was associated with improved stroke volume and cardiac output, with a trend towards reduced left ventricular stiffness. Both CANA and SIT trended towards improved perfusion compared to CON, but there were no differences between the two treatment groups. SIT was associated with improved capillary density with a trend towards improved arteriolar density compared to CANA. Both CANA and SIT were associated with increased expression of vascular endothelial cadherin compared to CON, without differences in treatment groups. SIT pigs had decreased 5′ adenosine monophosphate-activated protein kinase activation compared to CON and CANA. There was a trend towards increased endothelial nitric oxide synthase activation in the SIT group compared to CON. There were no differences in activation of extracellular signal-regulated kinase 1/2 across groups.
Conclusions: In the setting of chronic myocardial ischemia, canagliflozin is associated with improved cardiac function compared to sitagliptin, with similar effects on perfusion despite differences in microvascular collateralization.
Whole-Rock and Apatite Geochemistry of Late Triassic Plutonic Rocks in the Eastern Songpan-Ganzi Orogenic Belt: Petrogenesis and Implications for Tectonic Evolution
Authors: Yan Haoyu , Xu Zhiqin , Li Guangwei , Zheng Bihai , Gao Jianguo , Long Xiaoping 
Published: January 2024

Abstract
To constrain the late Triassic tectonic evolution of the Songpan-Ganzi orogenic belt, we present new whole-rock and in situ apatite geochemistry for plutonic rocks in its eastern margin. The Taiyanghe pluton can be classified into two rock types: dioritic and granitic rocks. The former exhibits low SiO2 and MgO contents but high Al2O3, Th, LREE contents, and Th/Yb and Th/Nb ratios, as well as low Ba/La and Ba/Th ratios and enriched Sr-Nd isotopic compositions, which, together with apatite geochemistry and Nd isotopes, indicate that they were derived from low degrees of partial melting of lithospheric mantle metasomatized by sediment-derived melts. The latter is characterized by high Sr and low Y and Yb, with elevated Sr/Y and (La/Yb)N ratios, implying an adakitic affinity. Notably, their similar Sr-Nd isotopic compositions indicate an origin from partial melts of a newly underplated lower crust. The Maoergai granitic rocks, characterized by high Sr and low Y and Yb contents with high Sr/Y and (La/Yb)N ratios, are indicative of adakitic rocks. In combination with the enriched whole-rock Sr-Nd isotopes and the apatite Nd isotopic data, we suggest that they were generated by the partial melting of the ancient thickened mafic lower crust. The Markam and Yanggonghai felsic granitoid rocks are peraluminous and similar to typical S-type granitoids, indicating an origin from remelting of the Triassic metasedimentary rocks. Based on the temporal-spatial relationship of the late Triassic plutonic rocks in the orogenic belt, we suggest that these rocks were formed in association with the roll-back and subsequent break-off of a subducted slab of the Paleo-Tethys Ocean. During the subduction, the formation of the Maoergai adakitic rocks was triggered by slab roll-back, whereas the magmatic “flare up” (ca. 216–200 Ma) was likely caused by slab break-off. This indicates that the final closure of the Paleo-Tethys Ocean happened in the end of the Triassic or Early Jurassic.
Petrogenesis and Tectonic Implication of Jurassic Granites in Central Guangdong, SE China: Constraints from Zircon U-Pb-Hf-O and Whole-Rock Geochemical and Sr-Nd Isotopic Data
Authors: Lai Zhiguang , Xu Yongxin , Xin Chunbo , Luo Xuewen 
Published: January 2024

Abstract
The origin and tectonic regime responsible for the inland Jurassic granites in Southeast (SE) China remain controversial. This study presents zircon secondary ion mass spectrometry (SIMS) U-Pb ages, in situ zircon Hf-O isotopes, and whole-rock geochemical and Sr-Nd isotopic data for the Fogang and Xinxing Batholiths in central Guangdong. Mineralogical and geochemical features indicate that these granites are high-K (&gt;4.8 wt% K2O at 72 wt% SiO2), calc-alkaline I-type granites. SIMS U-Pb analyses on magmatic zircons yield consistent ages ranging from 158 to 163 Ma, suggesting that the Fogang and Xinxing granites were emplaced in the period of 163–158 Ma. In addition, these granites have whole-rock initial Sr87/Sr86 ratios of 0.6802–0.7072 and negative εNd(t) values of −9.5 to −8.2, zircon negative εHf(t) values of −12.34 to −0.56, and high δ18O values of 7.64‰–10.08‰. The above features imply that the granites were most likely generated through the mixture of supracrustal sedimentary components with minor addition of mantle-derived magmas. Granites from the Fogang and Xinxing Batholiths in SE China should be derived from the Proterozoic crustal reworking due to asthenosphere upwelling or underplating and intrusion of mafic magmas. These Jurassic granites reflect anorogenic magmatism probably formed in an intraplate extensional setting resulted from the foundering of the flat slab beneath SE China.
Potentiometric determination of urea in fermented alcoholic beverages commonly consumed in India using biosensor by immobilization of urease on nylon membrane using Ammonium Ion Selective Electrode (AISE)
Authors: Attri Meenakshi , Kumari Seema 
Published: January 2024

Abstract
A method is described for construction of a highly sensitive potentiometric biosensor for detection of urea in some easily available fermented alcohols. The biosensor is based on covalent immobilization of urease enzyme nanoparticles on nylon membrane deposited on to ammonium ion selective electrode (AISE).The enzyme electrode was characterized by Fourier transform infra-reds pectroscopy (FTIR) and Field emission scanning electron microscopy (FESEM) techniques with particle size ranging between 0.09µm-0.49µm.in diameter.The biosensor showed optimum response within 20s at pH 5.5 in 0.05mM urea conc. in sodium phosphate buffer and 40˚C. It exhibited excellent sensitivity of 38 mV/decade and lower detection limit is 0.001 mM, and wider linear range from 0.001 to 0.80 mM. Analytical recovery of added urea which were found to be 99.8%, 101.04%, 108.35%, 103.9%, 98.99%, 104.7%, 99.93%, 102.34%, 101.76%, and 103.02%.The average urea conc. in beer is 22.23mg/L whereas in wine it is 0.7234 mg/L. The average pH is 3.795 in beer and in wine it is 3.85. The average urea conc. in beer is 22.23mg/L whereas in wine it is 0.7234mg/L, which is a very low amount as compared to that of found in milk (being compared with due to unavailability of permissible limit for alcohols). The working electrode was used weekly times over a period of 6 months, when stored at 4˚C. The potentiometric biosensor measured urea level in fermented alcoholic beverages, which correlated well with a standard potentiometric method (r =0.9898)
Macrophage-derived CCL1 targets CCR8 receptor in hepatic stellate cells to promote liver fibrosis through JAk/STAT pathway
Authors: Li Jun , Diao Shaoxi , Li Liangyun , Zhang Jintong , Ji Minglu , Sun Lijiao , Shen Wen-wen , Wu Shuai , Chen Zixiang , Huang Cheng 
Published: January 2024

Abstract
Liver fibrosis is caused by liver injury resulting from the wound healing response. According to recent research, the primary factor responsible for liver fibrosis is the activation of hepatic stellate cells (HSCs). C-C motif chemokine ligand 1 (CCL1) is one of several chemokine genes clustered on chromosome 17, which is involved in immune regulation and inflammatory processes. However, the role of CCL1 in liver fibrosis has not been reported. We found that CCL1 secreted by macrophages can target and activate the receptor protein C-C motif chemokine receptor 8 (CCR8) of HSCs, accelerating liver fibrosis progression by activating the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signalling pathway. This suggested that the CCL1-mediated regulation of CCR8 is an important event in liver fibrosis progression. In conclusion, this study identified a novel signalling axis, the CCL1/CCR8/JAK/STAT pathway, which regulates the activation and apoptosis of HSCs, thus providing a novel therapeutic strategy for liver fibrosis.
Clostridium cellabutyricum sp. nov., isolated from a Chinese liquor mud cellar, exhibiting probiotic potential and antibacterial activity against Pseudomonas aeruginosa
Authors: Qi Yin , Lu Xiao-Ling , Li Yingli , Liu Xiang-Ru , Nie Hao , Zhang Yuan-Jie , Wang Meng-Ling 
Published: January 2024

Abstract

Clostridium spp., which can produce short-chain fatty acids, represents potential probiotic candidates as promising adjuvant therapy against immune-associated disease via modulating host gut microbiome disorder. However, as obligate anaerobic bacteria, the strain resource of Clostridium spp. remains limited. A novel Clostridium bacterium, designated strain YQ-FP-027T, was isolated from a Chinese liquor mud cellar with the highest 16S rRNA gene sequence similarity (97.24%) with Clostridium intestinale DSM6191T. Characterization based on phylogenetic, phenotypic, chemotaxonomic, and genomic evidence was conducted. The probiotic profiles were determined both in vitro and in vivo. While its in vitro resistance to bile salts and acid was limited, the strain produced significant quantities of butyric acid (2001.92µg/g), and acetic acid (458.17µg/g). Its supernatant exhibited inhibitory effects on Pseudomonas aeruginosa bacterial cell growth. To evaluate its in vivo probiotic capacity, two chronic Pseudomonas aeruginosa Biofilm lung infection mouse models for both the preventative (pre-treatment) and therapeutic (post-treatment) applications of strain YQ-FP-027T were constructed. Treatment with YQ-FP-027T substantially reduced bacterial loads in lung tissue by 44.4% and 34.9% for the prevention and treatment models, respectively. Notably, upregulation of interleukin-10 expression and a significant increase in gut flora diversity were observed during YQ-FP-027T treatment. Further analysis of gut microbial diversity indicated an increase in beneficial genera such as Lachnospira and Prevotella, with a potential uptick in Lactobacillus abundance post-treatment in the prevention model, indicating the modulating ability of strain YQ-FP-027T on the gut microbiome as underlying mechanisms against Pseudomonas aeruginosa Biofilm infections. Characterization based on phylogenetic, phenotypic, chemotaxonomic, and genomic evidence demonstrated that strain YQ-FP-027T represents a novel species of the genus Clostridium, for which the name Clostridium cellabutyricum sp. nov., is proposed. Our results showed that strain holds great potential to provide a therapeutic alternative to alleviate the negative impacts of current infectious disease treatments.