bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Doxorubicin Loaded Aptamer S3-Linked DNA Tetrahedrons on Nasopharyngeal Carcinoma
Authors: Liu Xiwu , Jiang Bincan , Cheng Ailan , Guo Youwei , Wang Lei , Liu Weidong , Yin Wen , Li Yihan , Jiang Xingjun , Ren Caiping 
Published: December 2023

Objective Our research group in the early stage identified CD109 as the target of aptamer S3 in nasopharyngeal carcinoma (NPC). This study was to use S3 to connect DNA tetrahedron (DT) and load doxorubicin (Dox) onto DT to develop a targeted delivery system, and explore whether S3-DT-Dox can achieve targeted therapy for NPC. Methods Aptamer S3-conjugated DT was synthesized and loaded with Dox. The effects of S3-DT-Dox on NPC cells were investigated with laser confocal microscopy, flow cytometry, and MTS assays. A nude mouse tumor model was established from NPC 5-8F cells, and the in vivo anti-tumor activity of S3-DT-Dox was examined by using fluorescent probe labeling and hematoxylin–eosin staining. Results The synthesized S3-DT had high purity and stability. S3-DT specifically recognized 5-8F cells and NPC tissues in vitro. When the ratio of S3-DT to Dox was 1:20, S3-DT had the best Dox loading efficiency. The drug release rate reached the maximum (0.402 ± 0.029) at 48 h after S3-DT-Dox was prepared and mixed with PBS. S3-DT did not affect Dox toxicity to 5-8F cells, but reduced Dox toxicity to non-target cells. Meanwhile, S3-DT-Dox was able to specifically target the transplanted tumors and inhibit their growth in nude mice, with minor damage to normal tissues. Conclusion Our study highlights the ability and safety of S3-DT-Dox to target NPC cells and inhibit the development NPC. Graphical abstract
Nuclear face of Tau: an inside player in neurodegeneration
Authors: Younas Neelam , Saleem Tayyaba , Younas Abrar , Zerr Inga 
Published: December 2023

AbstractTau (Tubulin associated unit) protein is a major hallmark of Alzheimer’s disease (AD) and tauopathies. Tau is predominantly an axonal protein with a crucial role in the stabilization and dynamics of the microtubules. Since the discovery of Tau protein in 1975, research efforts were concentrated on the pathophysiological role of Tau protein in the context of the microtubules. Although, for more than three decades, different localizations of Tau protein have been discovered e.g., in the nuclear compartments. Discovery of the role of Tau protein in various cellular compartments especially in the nucleus opens up a new fold of complexity in tauopathies. Data from cellular models, animal models, and the human brain indicate that nuclear Tau is crucial for genome stability and to cope with cellular distress. Moreover, it’s nature of nuclear translocation, its interactions with the nuclear DNA/RNA and proteins suggest it could play multiple roles in the nucleus. To comprehend Tau pathophysiology and efficient Tau-based therapies, there is an urgent need to understand whole repertoire of Tau species (nuclear and cytoplasmic) and their functional relevance. To complete the map of Tau repertoire, understanding of various species of Tau in the nucleus and cytoplasm, identification if specific transcripts of Tau, isoforms and post-translational modifications could foretell Tau’s localizations and functions, and how they are modified in neurodegenerative diseases like AD, is urgently required. In this review, we explore the nuclear face of Tau protein, its nuclear localizations and functions and its linkage with Alzheimer’s disease.
Store of value or speculative investment? Market reaction to corporate announcements of cryptocurrency acquisition
Authors: Gimenes André D. , Colombo Jéfferson A. , Yousaf Imran 
Published: December 2023

AbstractIn this study, we analyze the stock market reaction to 35 events associated with 32 publicly traded companies from six countries that have announced cryptocurrency acquisitions, selling, or acceptance as a means of payment. Our analysis focuses on traditional firms whose core business is unrelated to blockchain or cryptocurrency. We find that the aggregate market reaction around these events is slightly positive but statistically insignificant for most event windows. However, when we perform heterogeneity analyses, we observe significant differences in market reaction between events with high (larger CARs) and low cryptocurrency exposure (lower CARs). Multivariate regressions show that the level of exposure to cryptocurrency ("skin in the game") is a critical factor underlying abnormal returns around the event. Further analyses reveal that economically meaningful acquisitions of BTC or ETH (relative to firm's total assets) drive the observed effect. Our findings have important implications for managers, investors, and analysts as they shed light on the relationship between cryptocurrency adoption and firm value.
Evaluation of adjuvants for reducing the risk of phytotoxicity in low-volume spray of propiconazole
Authors: Teng Lijie , Gao Tuqiang , Gu Anyu , Zhang Qizhen , Hu Maolin , Hao Jianjun , Li Xiaolin , Liu Pengfei 
Published: December 2023

AbstractUnmanned aerial vehicles (UAVs) have been increasingly employed for fungicide applications in plant disease control. However, due to weight limitations, the fungicides sprayed through UAVs must be in low volumes with high concentrations in many instances, which may result in potential phytotoxicity. Here we evaluate the safety of low-volume spray of chemicals on rice plants. The plants were sprayed with propiconazole emulsifiable concentrate (EC) at 250 g/L mixed with various adjuvants and applied at a low volume, which contained the fungicide at concentrations equivalent to or higher than that used in UAV application. The spray adjuvants included YS-20, Biaopu adjuvant, TriTek, Yipinsongzhi, AgriSolv-C100, and Hongyuyan. Potential phytotoxicity on rice plants was examined based on surface tension and crop growth. Additives suitable for a low-volume spray of propiconazole were also assessed on three rice varieties for phytotoxicity. The results showed that after 72 h of fungicide application at 2, 4, and 8 times the recommended dose of 7500 μg/mL for UAV spray, rice leaves exhibited abnormal growth, and the dry weight of rice significantly decreased 21 days after application. Phytotoxicity was evaluated on three rice varieties 5 days after spraying propiconazole EC at 2 × recommended dose with one of the spray adjuvants. The addition of 1% YS-20, Biaopu adjuvant, TriTek, and Yipinsongzhi significantly augmented the phytotoxicity. However, both AgriSolv-C100 and Hongyuyan significantly reduced the comprehensive index of phytotoxicity and, therefore, could be used for UAV applications.
Coping strategies and burden stress in caregivers of patients with schizophrenia and patients with bipolar disorder
Authors: Mohamed Rehab Serag , Mahmoud Nivert Zaki , Mahmoud Dalia Abdel Moneim , Eissa Mohamed Fekry , Zohdy Youssef Wael , Hafez Yassmin Shawky Rateb 
Published: December 2023

Abstract
Background
Mental illness has a great impact not only on patients but also on their carers as it makes them more prone to stress, interferes with their daily life activities, and can negatively affect their social, psychological, and physical health quality of life. They neglect seeking medical help that may negatively affect clinical outcome of patients and quality of their life. Therefore, it is important to evaluate the magnitude of burden on such caregivers and to investigate the coping strategies used by them in order to develop tailored biopsychosocial interventions to reduce their stress.

Objective
To assess the magnitude of burden and patterns of coping with stress in caregivers of patients with schizophrenia. To assess the magnitude of burden and patterns of coping with stress in caregivers of patients with bipolar affective disorder. To compare both the coping strategies and burden stress scores among carers of patients diagnosed with schizophrenia and bipolar patients.

Patients and methods
This cross-sectional study was done on 100 caregivers to assess burden in caregivers of schizophrenia patients and caregivers of bipolar patients, the coping style used by the caregivers of both groups and comparing between two groups.

Results
There was statistically significant difference found between bipolar group and schizophrenia group regarding coping inventory scores make fun and behavioral withdrawal, which showed higher median score in schizophrenia group than bipolar group. Burden of caring and coping with this burden, affect each other mutually. As, according to the results of our study, rural residence of caregiver, positive reassessment ≤ 12 and denial > 8 were found significantly associated with high burden in bipolar group. While employed caregivers were associated with high burden among schizophrenia group. Burden is higher in bipolar group than schizophrenia group.

Conclusion
The burden placed on caregivers has a significant impact on their capacity to offer service and care to patients. It also has an impact on how service providers adjust to and deal with their families' illnesses. Reduced caregiver load and increased awareness of the condition may help caregivers to use more flexible coping techniques.
Long-term Risk for Type 1 Diabetes and Obesity in Early Term Born Offspring: A Systematic Review and Meta-Analysis
Authors: Tsaitlin-Mor Lilah , Cahen-Peretz Adva , Bentov Yaakov , Ben-Shushan Tomer , Levine Hagai , Walfisch Asnat 
Published: December 2023

Abstract

Context
Prematurity increases the long-term risks for endocrine and metabolic morbidity of offspring, but there is uncertainty regarding the risks for early-term deliveries (370/7-386/7 weeks of gestation).

Objective
We aim to evaluate whether early-term deliveries increase the long-term risk for type 1 diabetes and obesity of offspring up to the age of 18 years compared with full-term children. PubMed, Medline, and EMBASE were searched. Observational cohort studies addressing the association between early-term delivery and long-term risk for type 1 diabetes and obesity, were included. Two independent reviewers extracted data and assessed risk of bias. Pooled relative risks (RRs) and heterogeneity were determined. Publication bias was assessed by funnel plots with Egger's regression line and contours, and sensitivity analyses were performed.

Results
Eleven studies were included following a screen of 7500 abstracts. All studies were scored as high quality according to the Newcastle–Ottawa Quality Assessment Scale. Early-term delivery was significantly associated with an increased risk for type 1 diabetes (RR 1.19, 1.13-1.25), while the association was weaker for overweight and obesity (RR 1.05, 0.97-1.12). It is challenging to determine whether the association between early-term births and long-term morbidity represents a cause and effect relationship or is attributable to confounders. Most of the included studies adjusted for at least some possible confounders.

Conclusion
Compared with full-term offspring, early-term delivery poses a modest risk for long-term pediatric type 1 diabetes. Our analysis supports that, whenever medically possible, elective delivery should be avoided before 39 completed weeks of gestation.
Adult-type Diffuse Gliomas
Authors: de la Fuente Macarena I. 
Published: December 2023

ABSTRACT

OBJECTIVE
This article highlights key aspects of the diagnosis and management of adult-type diffuse gliomas, including glioblastomas and IDH-mutant gliomas relevant to the daily practice of the general neurologist.

LATEST DEVELOPMENTS
The advances in molecular characterization of gliomas have translated into more accurate prognostication and tumor classification. Gliomas previously categorized by histological appearance solely as astrocytomas or oligodendrogliomas are now also defined by molecular features. Furthermore, ongoing clinical trials have incorporated these advances to tailor more effective treatments for specific glioma subtypes.

ESSENTIAL POINTS
Despite recent insights into the molecular aspects of gliomas, these tumors remain incurable. Care for patients with these complex tumors requires a multidisciplinary team in which the general neurologist has an important role. Efforts focus on translating the latest data into more effective therapies that can prolong survival.
2021 World Health Organization Classification of Brain Tumors
Authors: Meredith David M. , Pisapia David J. 
Published: December 2023

ABSTRACT

Objective
The classification of brain tumors is a rapidly evolving field that requires extensive integration of molecular diagnostic findings from an expanding set of platforms and assays. This article summarizes the schema presented in the 5th edition of the World Health Organization (WHO) classification of central nervous system (CNS) tumors while highlighting diagnostic molecular findings and discussing the strengths and weaknesses of commonly available testing modalities.

Latest Developments
Several major changes in practice were introduced with the 5th edition of the CNS WHO classification, including molecular grading of adult diffuse gliomas, the introduction of many new entities within the spectrum of pediatric gliomas and glioneuronal tumors, and the widespread adoption of methylation classes as useful or even necessary diagnostic criteria. Additionally, several revisions to nomenclature (eg, IDH-mutant gliomas) were introduced for simplicity and to disambiguate from other tumor types.

Essential Points
The classification of brain tumors continues to grow in complexity alongside our improved understanding of their nuanced molecular underpinnings.
Central Nervous System Lymphoma
Authors: Schaff Lauren 
Published: December 2023

ABSTRACT

OBJECTIVE
This article reviews the clinical presentation, diagnostic workup, staging, and treatment of primary central nervous system (CNS) lymphoma and common manifestations of secondary CNS lymphoma.

LATEST DEVELOPMENTS
Lymphoma can arise in the CNS de novo (primary CNS lymphoma) or as the result of systemic disease (secondary CNS lymphoma). Symptoms may include focal neurologic deficits related to the disease site, cognitive decline, and symptoms of increased intracranial pressure. Standard treatment may differ based on lymphoma subtype and location. A majority of CNS lymphoma is diffuse large B-cell subtype and exhibits aggressive behavior. First-line treatment is generally methotrexate-based polychemotherapy. Response rates to treatment are high, approximately 80% to 90% for primary CNS lymphoma, but relapse is common. Consolidation approaches including myeloablative chemotherapy followed by autologous stem cell rescue, nonmyeloablative chemotherapy, radiation, and medical maintenance regimens reduce rates of relapse. The recent development of targeted agents such as Bruton tyrosine kinase inhibitors and immunomodulatory strategies have shown promise in the treatment of CNS lymphoma. Immunotherapy in the form of checkpoint inhibitors and chimeric antigen receptor T cells is being studied. More indolent forms of lymphoma may be treated with radiation or targeted therapy.

ESSENTIAL POINTS
CNS lymphoma is an uncommon but clinically meaningful manifestation of extranodal lymphoma. The diagnosis requires a high level of suspicion for rapid initiation of potentially curative treatment.
Paraneoplastic Neurologic Syndromes
Authors: Graber Jerome J. 
Published: December 2023

ABSTRACTOBJECTIVEProgress is ongoing in understanding paraneoplastic neurologic disorders, with new syndromes and antibodies being described and more detailed evidence available to guide workup for diagnosis and treatment to improve outcomes. Many excellent reviews have summarized the molecular features of different antibodies, but this article emphasizes the clinical features of each syndrome that may help guide initial diagnosis and treatment, which often should occur before an antibody or cancer is found to confirm the diagnosis.LATEST DEVELOPMENTSRecent findings include updated diagnostic criteria with validated sensitivity and specificity, discovery of novel antibodies, and clinical findings that increase the likelihood of an underlying paraneoplastic disorder. Suggestive syndromes that have been recently identified include faciobrachial dystonic seizures and pilomotor auras in anti–leucine-rich glioma inactivated protein 1 encephalitis, extreme delta brush on EEG inN-methyl-d-aspartate (NMDA)-receptor encephalitis, déjà vu aura in anti–glutamic acid decarboxylase 65 (GAD65) encephalitis, and sleep disturbances in several disorders. In addition, there is confirmed utility of brain positron emission tomography (PET) and CSF markers, including carcinoembryonic antigen and oligoclonal bands, as well as improved tests for the presence of leptomeningeal cancer cells in CSF. Associations of cancer immunotherapies with paraneoplastic syndromes and herpes simplex virus encephalitis (and COVID-19) with NMDA-receptor encephalitis have been described.ESSENTIAL POINTSAll neurologists should be aware of advances regarding paraneoplastic neurologic syndromes, as patients can present with a wide variety of neurologic symptoms and earlier diagnosis and treatment can improve outcomes.
Management of Complications in Neuro-oncology Patients
Authors: Welch Mary R. 
Published: December 2023

ABSTRACT

OBJECTIVE
The purpose of this article is to familiarize the reader with the spectrum of neurologic and medical complications relevant to the care of patients with neurologic cancer while highlighting best practices to prevent morbidity and mortality. Topics include tumor-related epilepsy, vasogenic edema, complications of corticosteroid use, disruption of the hypothalamic-pituitary axis, venous thromboembolism, and opportunistic infection.

LATEST DEVELOPMENTS
In 2021, a joint guideline from the Society for Neuro-Oncology and the European Association of Neuro-Oncology reaffirmed recommendations first established in 2000 that patients with newly diagnosed brain tumors should not be prescribed an antiseizure medication prophylactically. For those with tumor-related epilepsy, monotherapy with a non–enzyme-inducing anticonvulsant is the preferred initial treatment, and levetiracetam remains the preferred first choice. Surveys of physician practice continue to demonstrate excessive use of glucocorticoids in the management of patients with both primary and metastatic central nervous system malignancy. This is particularly concerning among patients who require checkpoint inhibitors as the efficacy of these agents is blunted by concomitant glucocorticoid use, resulting in a reduction in overall survival. Finally, direct oral anticoagulants have been shown to be safe in patients with brain tumors and are now favored as first-line treatment among those who require treatment for venous thromboembolism.

ESSENTIAL POINTS
Medical care for patients impacted by primary and secondary central nervous system malignancy is complex and requires a committed team-based approach that routinely calls upon the expertise of physicians across multiple fields. Neurologists have an important role to play and should be familiar with the spectrum of complications impacting these patients as well as the latest recommendations for management.
Neurologic Complications of Cancer Immunotherapy
Authors: Wang Nancy 
Published: December 2023

ABSTRACT

Objective
Immunotherapeutic approaches have revolutionized cancer treatment with immune checkpoint inhibitors and adoptive T-cell therapy now approved to treat a variety of solid and hematologic malignancies. This article summarizes the distinctive neurologic side effects of these therapies as well as their management.

Latest Developments
Neurologic immune-related adverse events are rare but potentially serious complications of immune checkpoint inhibitors. Both peripheral and central nervous system disorders have been described, often necessitating a pause or cessation of immunotherapy. Immune effector cell–associated neurotoxicity syndrome is a potentially serious complication of chimeric antigen receptor T-cell therapy. While symptoms may be mild and self-limited, delirium, encephalopathy, seizures, focal neurologic deficits, and fulminant cerebral edema can be seen. Close neurologic monitoring is imperative. The mainstay of treatment for neurologic complications includes high-dose corticosteroids, although other immunomodulatory strategies may be used in severe or refractory cases.

Essential Points
The spectrum of neurologic complications of cancer immunotherapy is broad, encompassing both central and peripheral nervous system disorders, indolent as well as fulminant clinical presentations, and wide-ranging severity with variable response to treatment. Early identification and multidisciplinary management are crucial to balance neurologic recovery and antitumor control.
Rules of blood sampling for laboratory tests
Authors: Novikova Ol'ga 
Published: December 2023

There are rules that are mandatory when taking biological material for laboratory research. For the results of the study, not only the age, gender, race of the patient, factors of biological variation are important, but also the time of blood collection, physical activity, emotional state, the use of certain products, pharmacological preparations and biological additives.
Analysis of a sample taken with a violation may be more dangerous for the patient than the absence of a study. This is fraught with incorrect tactics of the doctor when monitoring the course and pharmacotherapy of an already known disease and diagnostic errors in primary and / or differential diagnosis.
A Regularized High-Dimensional Positive Definite Covariance Estimator with High-Frequency Data
Authors: Cui Liyuan , Hong Yongmiao , Li Yingxing , Wang Junhui 
Published: December 2023

This paper proposes a novel large-dimensional positive definite covariance estimator for high-frequency data under a general factor model framework. We demonstrate an appealing connection between the proposed estimator and a weighted group least absolute shrinkage and selection operator (LASSO) penalized least-squares estimator. The proposed estimator improves on traditional principal component analysis by allowing for weak factors, whose signal strengths are weak relative to idiosyncratic components. Despite the presence of microstructure noises and asynchronous trading, the proposed estimator achieves guarded positive definiteness without sacrificing the convergence rate. To make our method fully operational, we provide an extended simultaneous alternating direction method of multipliers algorithm to solve the resultant constrained convex minimization problem efficiently. Empirically, we study the monthly high-frequency covariance structure of the stock constituents of the S&P 500 index from 2008 to 2016, using all traded stocks from the NYSE, AMEX, and NASDAQ stock markets to construct the high-frequency Fama-French four and extended eleven economic factors. We further examine the out-of-sample performance of the proposed method through vast portfolio allocations, which deliver significantly reduced out-of-sample portfolio risk and enhanced Sharpe ratios. The success of our method supports the usefulness of machine learning techniques in finance. This paper was accepted by Agostino Capponi, finance. Funding: This work was supported by the Research Grants Council, University Grants Committee [Grants 11500119, 11505522, 11505721, and 21504818] and the National Natural Science Foundation of China (NSFC) Basic Scientific Center Project [Grant 71988101], entitled as “Econometric Modelling and Economic Policy Studies”, as well as NSFC [Grants 71803166 and 72173104]. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2022.04138 .