bluuebunny/crossref_metadata_2025_split huggingface dataset:
A machine learning-based approach to prognostic model of sepsis with PANoptosis-related genes and performance of scRNA-seq data to assess prognostic signatures
Authors: Shao Jinglin , He Haihong , Huang Tingting , Lan Xi , Cui Shengjin , Wu Yunfeng , Zhang Lijun , Guo Shixing , Liu Jiao , Li Shuping , Sun Xiang , Chen Lei , Zhou Yiwen , Song Chunli 
Published: November 2023

Abstract
Sepsis is a systemic inflammatory response syndrome caused by the invasion of pathogenic microorganisms such as bacteria into the body. PANoptosis is an inflammatory programmed cell death with key characteristics of pyroptosis, apoptosis, and/or neoptosis. At present, there is no strong evidence to suggest that the prognosis of sepsis is closely related to PANoptosis. In this study, 38 key differentially expressed genes(DEGs) were obtained by analyzing DEGs in sepsis microarray data GSE65685 and GSE95233 and crossing them with the PANopotosis gene set. Then, gene features were screened through univariate analysis, lasso regression analysis, and multivariate COX regression analysis to construct a prognosis model consisting of three predictive features: IKBKB, AIM2, and CTSG. We used Kaplan Meier (K-M) survival analysis, receiver operating characteristic (ROC) time curve analysis, internal validation, and principal component analysis to evaluate the performance of the prognostic model. In addition, sepsis patients were divided into high-risk and low-risk groups based on risk scores and gene set enrichment analysis (GSEA) results, and significant differences were found in multiple immune cell functions and immune related KEGG signaling pathways. Subsequently, scRNA seq data and immune cell infiltration analysis showed that the IKBKB and AMI2 genes were highly expressed in all immune cells of sepsis patients, while the CTSG gene was mainly highly expressed in monocytes, neutrophils, NK cells, and proliferative T cells. In the analysis of target genes for 16 immunosuppressive drugs, only CSF3 was highly expressed in high-risk patients, indicating that CSF3 may be the most promising target for treating sepsis.
Causal relationships between delirium and Parkinson’s Disease: a bidirectional two-sample Mendelian randomization study
Authors: Bai Xiaoliang , Zhang Anna , Zhou Qingbo , Wang Hongli 
Published: November 2023

Abstract
Background
Previous observational studies have suggested a notably elevated prevalence of delirium in individuals diagnosed with Parkinson's disease (PD), thereby implying a potential increased susceptibility to delirium among PD patients. However, it is imperative to acknowledge that observational studies inherently possess limitations, rendering it arduous to establish a definitive causal or reverse causal association between delirium and PD.
Methods
To explore the relationship between delirium and PD, a bidirectional two-sample Mendelian randomization (MR) was conducted using summary statistics obtained from genome-wide association studies. The main analysis was performed using the inverse-variance weighted (IVW) method, with further analyses conducted using MR Egger, weighted median, and weighted mode to ensure accuracy of findings. Additionally, Cochran's Q statistics and MR Egger intercept were utilized to assess heterogeneity and horizontal pleiotropy, respectively.
Results
According to the results obtained from the IVW model, no compelling evidence was found to support a potential causal association between delirium and PD (IVW: odds ratio [OR]: 0.996, 95% confidence interval [CI] = 0.949–1.043, P = 0.845). Additionally, in the reverse direction, based on the results obtained from the IVW model, no significant evidence was found to support a causal association between PD and delirium (IVW: OR: 1.078, 95%CI = 0.960–1.204, p = 0.225). A sensitivity analysis verified the reliability of the results.
Conclusion
According to the MR findings, a bidirectional causal relationship between delirium and PD is not observed. It is crucial to conduct further research in clinical practice to investigate the association between delirium and the risk of PD.
Morphologically graded scalable nanoarchitected materials
Authors: Kagias Matias , Greer Julia R. 
Published: November 2023

Abstract
Nanoarchitected materials present a frontier in materials science, offering unique properties stemming from meticulously controlled nanoscale geometries. Despite considerable advancements, elucidating the connections between nanoscale architecture and macroscopic mechanical properties remains a complex challenge. In this study, we employ cryogenic 3D X-ray ptychography alongside \textit{in situ} mechanical testing of nanoarchitected pillars (diameter of approximately 40 micrometers) to illuminate the intricate interplay between morphological features and mechanical response in scalable polymer-based nano-architected metamaterials. These materials, endowed with features on the order of (approximately 500 nanometers), were created using metasurface enabled holographic lithography, and exhibited specific energy absorption in the order of 48 kJ/kg. Our analysis revealed a pronounced correlation between two spatially separated deformation modes, specifically barreling versus layer-by-layer collapse, and the principal curvatures inherent to the material's nanoscale architecture. This morphological variation demonstrates the feasibility of scalable functionally graded nanoarchitected materials with holographic lithography.
User Experience Design Methodologies for Building User Interfaces for Teleround System for Intensive Care Units
Authors: Moura Ana Beatriz Frade , Silva Lyvia Mota , Motter Fabiane Raquel , Silva Izadora Coelho , Silva William Aparecido Santos , Leao Beatriz de Faria , Col Shoraya Virginio Carneiro Dal , Gadenz Sabrina Dalbosco 
Published: November 2023

Abstract
Integrating design methods with telehealth intervention development offers an opportunity to understand user needs and to address potential barriers to using new digital tools.
Objective: This study aimed to apply user experience (UX) design methodologies for the construction of interfaces with users of the Mangará Digital Telemedicine platform, specifically for carrying out Telerounds with Intensive Care Units (ICUs) in remote locations.
Methods: This is a methodological study for developing a telemedicine platform. We used the Double Diamond Model to guide the design process. This method consists of four stages (Discover, Define, Develop, and Deliver), which include the following steps: 1. Construction of the initial vision of the Product Discovery journey through the CSD Matrix (Certainties, Assumptions, and Doubts); 2. Desk Research (DR); 3. Rapid Ethnographic research; 4. Comparison with previous projects using the methodology of “benchmarking”; 5. Creation of personas and empathy maps 6. Situational Diagnosis of ICUs. 7. User journey mapping; 8. Initial creation of the digital product using the Sitemap and UserFlow tools. 9. Construction of the usability of wireframes according to Nielsen's heuristics.
Results: The use of UX methodologies for the design of the user interface was necessary for the construction of the Mangará Digital Telerounds platform since it allowed the development of a friendly platform that meets the expectations and needs of its users.
Conclusion: This work demonstrates the importance of using UX methodologies to design the interfaces of electronic medical record systems in high-demand and complex environments such as ICUs.
Transcriptome data analysis provides insights into the conservation of Michelia lacei, a Plant Species with Extremely Small Populations distributed in Yunnan Province, China
Authors: Liu Yang , Cai Lei , Sun Weibang 
Published: November 2023

Abstract
Background
Michelia lacei W.W.Smith (Magnoliaceae) is an evergreen tree with high ornamental and scientific value. It was classified as a Plant Species with Extremely Small Populations (PSESP) by the Yunnan Provincial Government in both 2012 and 2021, due to the extremely small population size and narrow geographical distribution. Currently, research on M. lacei is limited to assessing the conservation status and population ecology, and to date there is no population genetics study. In this study, transcriptome data analysis was conducted on a total of 64 individuals from 7 populations of wild and cultivated M. lacei, resulting in the generation of 104,616 single-nucleotide polymorphisms (SNPs) that can be used in subsequent analyses.
Results
The population structure of M. lacei was analyzed using three methods, and the existing populations could be divided into three genetic clusters. In an analysis of population genetic diversity, the average π, HO, HE, FIS, and Tajima's D calculated across all populations were 0.298, 0.190, 0.702, 0.358, and 1.090, respectively. The population dynamics also reflected population changes in M. lacei during two past glaciations. The population size is thought to have stabilized about 10,000 years ago, however, there are now only 52 individuals of M. lacei known from the wild in China. Environmental changes have also promoted adaptive evolution of the species, as analysis of outlier loci indicates that some populations have high potential to adapt to the adverse environments, providing valuable genetic resources for the conservation of this species.
Conclusions
Our study clearly reveals the genetic background of M. lacei in different populations and the genetic diversity among the populations. Past gene flow between populations also reflects the causes of the current genetic structure. Combining inference of population history and adaptive analysis of M. lacei provides new insights into the conservation of genetic resources and facilitate the recovery of population size in this threatened plant species.
Treatment of type 5 acromioclavicular separation with minimal invasive closed-loop double-endobutton technique
Authors: Yang Wei-Hsiang , Liu Chung-Ting 
Published: November 2023

Abstract
Background:
Acromioclavicular (AC) separation ranks among the prevalent shoulder girdle injuries, accounting for 50% of all sports-related shoulder ailments. Surgical intervention is advocated for type IV-VI injuries. AC joint congruity and the anatomical integrity of the coracoclavicular (CC) ligament complex are prerequisites for robust scar tissue healing. As such, the aim of our study was to describe our technique of indirect osteosynthesis, using a minimally invasive closed-loop double endobutton (TightRope) technique for fixation of type 5 AC separation, and to evaluate the short-term clinical outcomes and complications.
Methods:
Fifty-one patients with a Rockwood type 5 AC separation were treated surgically using the TightRope fixation, between January 2017 and December 2021. Clinical and radiological results were assessed using the Constant-Murley score, with concurrent documentation of any complications.
Results:
In this study, the distribution of cases across the respective groups was as follows: 16 cases in the well-reduced group (31.4%), 5 cases in the incomplete reduction group (9.8%), 20 cases in the partial loss of reduction group (39.2%), and 10 cases in the failure of reduction group (19.6%). Notably, there were no statistically significant differences observed among the first three groups, whereas the complete loss of reduction group demonstrated a significantly lower Constant-Murley score.
Conclusion:
The Tightrope system offers strength for Rockwood type 5 AC separation, yielding favorable clinical outcomes; however, radiographic findings may not be entirely satisfactory. Partial reduction loss had minimal impact, but complete loss had inferior results. Precise Tightrope placement, ensuring button perpendicularity and avoiding coracoid tip positioning is crucial.
Neoadjuvant sintilimab and chemotherapy for resectable esophageal squamous cell carcinoma: a prospective, single-arm, phase II study
Authors: Jincheng Guo , chengrui Qiao , jiabin Lu , shiqiang Yang , boyi Zhang , dongxia Tang , hui Pang 
Published: November 2023

Abstract
Background: Combining PD-1 blockade with chemotherapy has widely used in first line treatment of metastatic esophageal squamous cell carcinoma (ESCC). However, their efficacy as neoadjuvant therapy in resectable ESCC is little known. This study was performed to assess the activity and safety profile of the combination of sintilimab and chemotherapy in the neoadjuvant treatment of ESCC.
Methods: In this single-arm, phase II study, we recruited patients with histopathologically diagnosed resectable ESCC who had clinical cT1-3/N0-1M0 (stage II-Ⅲ). Patients were given sintilimab (200mg, iv, d1) in combined with chemotherapy (nab-paclitaxel 260 mg/m2, d1 and cisplatin 75 mg/m2, d1-3) every 3 weeks for 2 cycles. The primary endpoint was pathological complete response (pCR).
Results：Between Nov 2020 and Nov 2022, 29 patients were enrolled and 27 patients completed the two cycles of neoadjuvant therapy. A total of 21 patients underwent surgery without treatment-related surgical delay. The major pathologic response (MPR) rate was 42.9% (9/21) and the pathologic complete response (pCR) rate was 28.6% (6/21). The most frequent grade 3 or 4 treatment-related adverse events (TRAE) were leukopenia (26.7%), neutropenia (20%) and pneumonia (6.7%). There was no surgical delays or unexpected surgical complications related to drug toxicity.
Conclusions: The combination of sintilimab and chemotherapy shows an encouraging pCR rate and a favorable safety profile in this study, which indicated this treatment regimen may become an alternative option in the neoadjuvant treatment of resectable esophageal squamous cell carcinoma, especially for Chinese patients.
Investigation of experimental for optical properties of molybdenum trioxide (MoO3) thin films using neural networks
Authors: Mohamed R. A. , El-Nahass M. M. , El-Bakry M. Y. , El-Dahshan El-Sayed A. , Aamer E. H. , Habashy D. M. 
Published: November 2023

Abstract

Nowadays, transition metal oxide (TMO), the semi-conductor molybdenum trioxide (MoO3) is under investigation. MoO3 is a good choice because of its many industrial applications and alluring qualities. It has a significant role as an industrial catalyst and has electrochromic properties. The first part is an experimental study of the optical behaviour of the above mentioned thin film onto various substrates. X-ray diffraction (XRD) analysis revealed an orthorhombic structure. Measurements were obtained of the average crystallite size and dislocation density, which were 132.8 nm and 5.7* 10− 4 nm-2, respectively. With the use of spectrophotometric measurements of transmittance (T) and reflectance (R) at normal incidence in the 300–1400 nm wavelength range at 310 nm thickness, the optical behaviour of the mentioned film onto various substrates was examined. The dispersion and oscillator energies were measured using the single oscillator model employing a Wemple–DiDomenico relation and indirect optical transitions with corresponding h ע energy gaps were identified for glass and quartz substrates. The second part introduces estimation optical behaviour of the above mentioned thin film using ANN model. Experimental data is used as inputs. The ANN modelling outputs optical characterization provide excellent results. Error values support the success of the modelling process (MSE > 10− 1). Moreover, the theoretical equation describing the experimental results was obtained depending on the ANN model which represents the relation between the inputs and outputs. According to this research, the ANN model can be utilized as an efficient tool to simulate and predict the optical parameters of the above mentioned film. Additionally, it can establish strong connections between theoretical and experimental fields.
Enterobacter and Pseudomonas: two dominant players in the rhizosphere phosphate-solubilizing bacterial communities of forage grasses adapted to alkaline-sodic soils of the flooding pampa
Authors: Dip Diana Patricia , Sannazzaro Analía Inés , Otondo José , Pistorio Mariano , Estrella María Julia 
Published: November 2023

Abstract
Cultivable phosphate solubilizing bacteria (PSB) communities associated to native (Sporobolus indicus) and exotic (Panicum coloratum) forage grasses adapted to alkaline-sodic soils of the flooding pampa were analyzed. PSB represented 2–14% of cultivable rhizobacteria and Box-PCR fingerprinting revealed a high genetic diversity in both rhizospheres. Taxonomic identification by MALDI-TOF showed that PSB populations of P. coloratum and S. indicus rhizospheres are dominated by the phylum Proteobacteria (92,51% and 96,60% respectively) and to a lesser extent (< 10%), by the phyla Actinobacteria and Firmicutes. At the genus level, both PSB populations were dominated by Enterobacter and Pseudomonas. Siderophore production, nitrogen fixation and indoleacetic acid production were detected in a variety of PSB genera of both plant species. A higher proportion of siderophore and IAA producers were associated to P. coloratum than S. indicus, probably reflecting a greater dependence of the exotic species on rhizospheric microorganisms to satisfy its nutritional requirements in soils of the flooding pampa. This study contributes to the knowledge of the taxonomic and functional diversity of PSB that can be cultivated in environments that have not been explored yet, such as alkaline-sodic soils that impose nutritional limitations for plant growth. Likewise, the results obtained on the PSB community of both plant species constitute valuable information and a starting point to advance in the development of efficient biofertilizers for forage grasses adapted to alkaline-sodic environments and thus reduce the environmental impact of chemical fertilizers.
The Consequences of Economic Growth, Energy Use, and Agricultural Methane Emissions on Environmental Deterioration in Sweden.
Authors: Damak Obadiah Ibrahim , GÜNGÖR Hasan 
Published: November 2023

Abstract
The purpose of this study is to look into how environmental deterioration in Sweden changed from 1990 to 2021 as a result of energy use, economic growth, and agricultural methane emissions. The empirical analysis makes use of various econometric methods, including the bounds test for autoregressive distributed lags (ARDL). The tests for Granger causality and FMOLS are applied to the data. At levels, the variables are non-stationary, other than renewable energy, in light of the results of the unit root and stationarity tests, despite the fact that they remain stationary in the I (1) or first-differences form. The five variables are shown to be related over the long term by the ARDL limits F-test. Additionally, both a rise in the usage of fossil fuels and clean energy usage has a major long-term impact on environmental deterioration. The coefficients of the estimated error correction component are also negative and statistically significant. Additionally, the research employs Granger causality tests to investigate the variables' causal connections. The outcomes demonstrated that use of clean energy does not contribute to CO2 emissions, whereas GDP, fossil fuel consumption, and agricultural methane emission do. The study’s also addressed a vacuum in the literature by using the ARDL method to look at this important topic for Sweden.
Spatial accessibility and travel to pharmacy in Detroit and its suburbs
Authors: Li Xiaomeng , Vojnovic Igor , Ligmann-Zielinska Arika 
Published: November 2023

Abstract
Community pharmacies are a critical part of the health care provision system. Yet less is understood about the spatial accessibility to pharmacies and how people travel to reach these services. This study compared spatial accessibility and actual travel to different types of pharmacies in sampled neighborhoods across the Detroit Region, including Urban Detroit neighborhoods with a concentration of low-income African Americans and predominantly high-income white suburbs. The results showed that, overall, low-income African American urban communities face a greater burden in reaching pharmacy services needed. Compared to suburban residents, Urban Detroit residents had higher access to independent pharmacies, but much lower access to national chains, which most residents relied on. In fact, Urban Detroit residents bypassed nearby local independent pharmacies and traveled long distances to use farther locations, primarily national chains. They also tended to shop around more for services. To our knowledge, this is the first study that has compared spatial access and actual travel to different types of pharmacies. It demonstrates that methodologically, it is important to distinguish spatial accessibility among different pharmacy types. In an urban context, supposedly good spatial access considering all pharmacies together may mask excessive burden in reaching the pharmacy services of choice in low-income minority communities, as in the case of Urban Detroit.
Epidemiological characteristics of common respiratory viruses among children in Hangzhou, China: comparison of COVID-19 before, during and after the pandemic
Authors: Zhou Jianming , Sun Yanhong , Shen Hao , Yang Liuqiao , Ye Qing , Zhu Zhiwen 
Published: November 2023

Abstract
Background: Non-Pharmaceutical Interventions (NPIs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) not only curbed the spread of novel coronavirus (COVID-19) but also affected common respiratory viruses infected by children. The purpose of this study was to explore the effect of NPIs on common respiratory viruses in children.
Methods: Samples of children diagnosed with respiratory tract infection in Children's Hospital affiliated with Zhejiang University from January 2019 to August 2023 were collected, and ADV, Flu A, Flu B and RSV were detected. To analyze the changes in common respiratory viruses in children in three stages: pre-COVID-19, pandemic and late. Statistical analysis was carried out with R software.
Results: From January 2019 to August 2023, a total of 567,858 samples were tested, including 308,163 males, accounting for 54.26%, and 259,695 females, accounting for 45.73%. Among them, there were 173,956 positive samples (30.63%), 40,279 ADV-positive samples (23.15%), 78,580 Flu A-positive samples (45.17%), 26,888 Flu B-positive samples (15.45%) and 28,209 RSV-positive samples (16.21%). Among children of different ages in Hangzhou before, during and after COVID-19, the highest total detection rate of respiratory virus was 1-3 years old (accounting for 40.24%), followed by 4-6 years old (accounting for 26.59%). The distribution in different seasons shows that the number of children infected with respiratory viruses reaches a peak in winter and spring. Compared with 2019 (35.60%) before the COVID-19 epidemic, the total detection rate of common respiratory viruses in children was lower during the COVID-19 pandemic (2020-2022) (27.89%, 22.71%, 24.63%), and it was increased in 2023 while after NPIs were cancelled (accounting for 33.75%).
Conclusion: NPI measures can effectively reduce the spread of common respiratory viruses, but at the same time, they will weaken human immunity to these respiratory viruses.