bluuebunny/crossref_metadata_2025_split huggingface dataset:
A system for inducible mitochondria-specific protein degradation in vivo
Authors: Sanyal Swastika , Kouznetsova Anna , Ström Lena , Björkegren Camilla 
Published: February 2024

AbstractTargeted protein degradation systems developed for eukaryotes employ cytoplasmic machineries to perform proteolysis. This has prevented mitochondria-specific analysis of proteins that localize to multiple locations, for example, the mitochondria and the nucleus. Here, we present an inducible mitochondria-specific protein degradation system in Saccharomyces cerevisiae based on the Mesoplasma florum Lon (mf-Lon) protease and its corresponding ssrA tag (called PDT). We show that mitochondrially targeted mf-Lon protease efficiently and selectively degrades a PDT-tagged reporter protein localized to the mitochondrial matrix. The degradation can be induced by depleting adenine from the medium, and tuned by altering the promoter strength of the MF-LON gene. We furthermore demonstrate that mf-Lon specifically degrades endogenous, PDT-tagged mitochondrial proteins. Finally, we show that mf-Lon-dependent PDT degradation can also be achieved in human mitochondria. In summary, this system provides an efficient tool to selectively analyze the mitochondrial function of dually localized proteins.
Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail
Authors: Sexton Joel A. , Potchernikov Tony , Bibeau Jeffrey P. , Casanova-Sepúlveda Gabriela , Cao Wenxiang , Lou Hua Jane , Boggon Titus J. , De La Cruz Enrique M. , Turk Benjamin E. 
Published: February 2024

AbstractCofilin family proteins have essential roles in remodeling the cytoskeleton through filamentous actin depolymerization and severing. The short, unstructured N-terminal region of cofilin is critical for actin binding and harbors the major site of inhibitory phosphorylation. Atypically for a disordered sequence, the N-terminal region is highly conserved, but specific aspects driving this conservation are unclear. Here, we screen a library of 16,000 human cofilin N-terminal sequence variants for their capacity to support growth inS. cerevisiaein the presence or absence of the upstream regulator LIM kinase. Results from the screen and biochemical analysis of individual variants reveal distinct sequence requirements for actin binding and regulation by LIM kinase. LIM kinase recognition only partly explains sequence constraints on phosphoregulation, which are instead driven to a large extent by the capacity for phosphorylation to inactivate cofilin. We find loose sequence requirements for actin binding and phosphoinhibition, but collectively they restrict the N-terminus to sequences found in natural cofilins. Our results illustrate how a phosphorylation site can balance potentially competing sequence requirements for function and regulation.
A genome and gene catalog of the aquatic microbiomes of the Tibetan Plateau
Authors: Cheng Mingyue , Luo Shuai , Zhang Peng , Xiong Guangzhou , Chen Kai , Jiang Chuanqi , Yang Fangdian , Huang Hanhui , Yang Pengshuo , Liu Guanxi , Zhang Yuhao , Ba Sang , Yin Ping , Xiong Jie , Miao Wei , Ning Kang 
Published: February 2024

AbstractThe Tibetan Plateau supplies water to nearly 2 billion people in Asia, but climate change poses threats to its aquatic microbial resources. Here, we construct the Tibetan Plateau Microbial Catalog by sequencing 498 metagenomes from six water ecosystems (saline lakes, freshwater lakes, rivers, hot springs, wetlands and glaciers). Our catalog expands knowledge of regional genomic diversity by presenting 32,355 metagenome-assembled genomes that de-replicated into 10,723 representative genome-based species, of which 88% were unannotated. The catalog contains nearly 300 million non-redundant gene clusters, of which 15% novel, and 73,864 biosynthetic gene clusters, of which 50% novel, thus expanding known functional diversity. Using these data, we investigate the Tibetan Plateau aquatic microbiome’s biogeography along a distance of 2,500 km and >5 km in altitude. Microbial compositional similarity and the shared gene count with the Tibetan Plateau microbiome decline along with distance and altitude difference, suggesting a dispersal pattern. The Tibetan Plateau Microbial Catalog stands as a substantial repository for high-altitude aquatic microbiome resources, providing potential for discovering novel lineages and functions, and bridging knowledge gaps in microbiome biogeography.
Giant electrostriction-like response from defective non-ferroelectric epitaxial BaTiO3 integrated on Si (100)
Authors: Parate Shubham Kumar , Vura Sandeep , Pal Subhajit , Khandelwal Upanya , Sandilya Ventrapragada Rama Satya , Rai Rajeev Kumar , Molleti Sri Harsha , Kumar Vishnu , Patil Girish , Jain Mudit , Mallya Ambresh , Ahmadi Majid , Kooi Bart , Avasthi Sushobhan , Ranjan Rajeev , Raghavan Srinivasan , Chandorkar Saurabh , Nukala Pavan 
Published: February 2024

AbstractLead-free, silicon compatible materials showing large electromechanical responses comparable to, or better than conventional relaxor ferroelectrics, are desirable for various nanoelectromechanical devices and applications. Defect-engineered electrostriction has recently been gaining popularity to obtain enhanced electromechanical responses at sub 100 Hz frequencies. Here, we report record values of electrostrictive strain coefficients (M31) at frequencies as large as 5 kHz (1.04×10−14m2/V2at 1 kHz, and 3.87×10−15m2/V2at 5 kHz) using A-site and oxygen-deficient barium titanate thin-films, epitaxially integrated onto Si. The effect is robust and retained upon cycling upto 6 million times. Our perovskite films are non-ferroelectric, exhibit a different symmetry compared to stoichiometric BaTiO3and are characterized by twin boundaries and nano polar-like regions. We show that the dielectric relaxation arising from the defect-induced features correlates well with the observed giant electrostriction-like response. These films show large coefficient of thermal expansion (2.36 × 10−5/K), which along with the giantM31implies a considerable increase in the lattice anharmonicity induced by the defects. Our work provides a crucial step forward towards formulating guidelines to engineer large electromechanical responses even at higher frequencies in lead-free thin films.
Durable Janus membrane with on-demand mode switching fabricated by femtosecond laser
Authors: Cui Zehang , Zhang Yachao , Zhang Zhicheng , Liu Bingrui , Chen Yiyu , Wu Hao , Zhang Yuxuan , Cheng Zilong , Li Guoqiang , Yong Jiale , Li Jiawen , Wu Dong , Chu Jiaru , Hu Yanlei 
Published: February 2024

AbstractDespite their notable unidirectional water transport capabilities, Janus membranes are commonly challenged by the fragility of their chemical coatings and the clogging of open microchannels. Here, an on-demand mode-switching strategy is presented to consider the Janus functionality and mechanical durability separately and implement them by simply stretching and releasing the membrane. The stretching Janus mode facilitates unidirectional liquid flow through the hydrophilic micropores-microgrooves channels (PG channels) fabricated by femtosecond laser. The releasing protection mode is designed for the in-situ closure of the PG channels upon encountering external abrasion and impact. The protection mode imparts the Janus membrane robustness to reserve water unidirectional penetration under harsh conditions, such as 2000  cycles mechanical abrasion, 10 days exposure in air and other rigorous tests (sandpaper abrasion, finger rubbing, sand impact and tape peeling). The underlying mechanism of gridded grooves in protecting and enhancing water flow is unveiled. The Janus membrane serves as a fog collector to demonstrate its unwavering mechanical durability in harsh real-world conditions. The presented design strategy could open up new possibilities of Janus membrane in a multitude of applications ranging from multiphase separation devices to fog harvesting and wearable health-monitoring patches.
Phase-engineered synthesis of atomically thin te single crystals with high on-state currents
Authors: Zhou Jun , Zhang Guitao , Wang Wenhui , Chen Qian , Zhao Weiwei , Liu Hongwei , Zhao Bei , Ni Zhenhua , Lu Junpeng 
Published: February 2024

AbstractMultiple structural phases of tellurium (Te) have opened up various opportunities for the development of two-dimensional (2D) electronics and optoelectronics. However, the phase-engineered synthesis of 2D Te at the atomic level remains a substantial challenge. Herein, we design an atomic cluster density and interface-guided multiple control strategy for phase- and thickness-controlled synthesis of α-Te nanosheets and β-Te nanoribbons (from monolayer to tens of μm) on WS2 substrates. As the thickness decreases, the α-Te nanosheets exhibit a transition from metallic to n-type semiconducting properties. On the other hand, the β-Te nanoribbons remain p-type semiconductors with an ON-state current density (ION) up to ~ 1527 μA μm−1 and a mobility as high as ~ 690.7 cm2 V−1 s−1 at room temperature. Both Te phases exhibit good air stability after several months. Furthermore, short-channel (down to 46 nm) β-Te nanoribbon transistors exhibit remarkable electrical properties (ION = ~ 1270 μA μm−1 and ON-state resistance down to 0.63 kΩ μm) at Vds = 1 V.
A 30-m annual corn residue coverage dataset from 2013 to 2021 in Northeast China
Authors: Dong Yi , Xuan Fu , Huang Xianda , Li Ziqian , Su Wei , Huang Jianxi , Li Xuecao , Tao Wancheng , Liu Hui , Chen Jiezhi 
Published: February 2024

AbstractCrop residue cover plays a key role in the protection of black soil by covering the soil in the non-growing season against wind erosion and chopping for returning to the soil to increase organic matter in the future. Although there are some studies that have mapped the crop residue coverage by remote sensing technique, the results are mainly on a small scale, limiting the generalizability of the results. In this study, we present a novel corn residue coverage (CRC) dataset for Northeast China spanning the years 2013–2021. The aim of our dataset is to provide a basis to describe and monitor CRC for black soil protection. The accuracy of our estimation results was validated against previous studies and measured data, demonstrating high accuracy with a coefficient of determination (R2) of 0.7304 and root mean square error (RMSE) of 0.1247 between estimated and measured CRC in field campaigns. In addition, it is the first of its kind to offer the longest time series, enhancing its significance in long-term monitoring and analysis.
Multimodal single-neuron, intracranial EEG, and fMRI brain responses during movie watching in human patients
Authors: Keles Umit , Dubois Julien , Le Kevin J. M. , Tyszka J. Michael , Kahn David A. , Reed Chrystal M. , Chung Jeffrey M. , Mamelak Adam N. , Adolphs Ralph , Rutishauser Ueli 
Published: February 2024

AbstractWe present a multimodal dataset of intracranial recordings, fMRI, and eye tracking in 20 participants during movie watching. Recordings consist of single neurons, local field potential, and intracranial EEG activity acquired from depth electrodes targeting the amygdala, hippocampus, and medial frontal cortex implanted for monitoring of epileptic seizures. Participants watched an 8-min long excerpt from the video “Bang! You’re Dead” and performed a recognition memory test for movie content. 3 T fMRI activity was recorded prior to surgery in 11 of these participants while performing the same task. This NWB- and BIDS-formatted dataset includes spike times, field potential activity, behavior, eye tracking, electrode locations, demographics, and functional and structural MRI scans. For technical validation, we provide signal quality metrics, assess eye tracking quality, behavior, the tuning of cells and high-frequency broadband power field potentials to familiarity and event boundaries, and show brain-wide inter-subject correlations for fMRI. This dataset will facilitate the investigation of brain activity during movie watching, recognition memory, and the neural basis of the fMRI-BOLD signal.
Cross-talk between disulfidptosis and immune check point genes defines the tumor microenvironment for the prediction of prognosis and immunotherapies in glioblastoma
Authors: Zhou Yanjun , Qin Xue , Hu Qunchao , Qin Shaolei , Xu Ran , Gu Ke , Lu Hua 
Published: February 2024

AbstractDisulfidptosis is a condition where dysregulated NAPDH levels and abnormal accumulation of cystine and other disulfides occur in cells with high SLC7A11 expression under glucose deficiency. This disrupts normal formation of disulfide bonds among cytoskeletal proteins, leading to histone skeleton collapse and triggering cellular apoptosis. However, the correlation between disulfidptosis and immune responses in relation to glioblastoma survival rates and immunotherapy sensitivity remains understudied. Therefore, we utilized The Cancer Genome Atlas and The Chinese Glioma Genome Atlas to identify disulfidptosis-related immune checkpoint genes and established an overall survival (OS) prediction model comprising six genes: CD276, TNFRSF 14, TNFSF14, TNFSF4, CD40, and TNFRSF18, which could also be used for predicting immunotherapy sensitivity. We identified a cohort of glioblastoma patients classified as high-risk, which exhibited an upregulation of angiogenesis, extracellular matrix remodeling, and epithelial-mesenchymal transition as well as an immunosuppressive tumor microenvironment (TME) enriched with tumor associated macrophages, tumor associated neutrophils, CD8 + T-cell exhaustion. Immunohistochemical staining of CD276 in 144 cases further validated its negative correlation with OS in glioma. Disulfidptosis has the potential to induce chronic inflammation and an immunosuppressive TME in glioblastoma.
A real-time field bus architecture for multi-smart-motor servo system
Authors: Huang Zhichao , Qiu Song , Wang Bangji , Liu Qingxiang 
Published: February 2024

AbstractThe multi-motor servo system (MMSS) is an electro-mechanical system widely used in various fields, including electric vehicles, robotics, and industrial machinery. Depending on the application, the number of motors in the system can range from several dozens to tens of thousands, which imposes additional communication demands. Thus, ensuring synchronization and control precision of the system requires addressing the challenge of guaranteeing the performance and reliability of communication among motors in the MMSS. In this paper, we design a smart servo motor (SSM) to upgrade the system to the multi-smart-motor servo system (MSMSS) based on a distributed real-time field bus architecture, namely, Multi-Motor Bus (MMB) architecture. The proposed MMB architecture is lightweight and stable, providing real-time support for Control Area Network connections to a central user computer and inter-integrated circuit connections to SSM units. This MMB architecture facilitates the synchronization of command transmission across SSMs and ensures the consistency of motors in the MSMSS. Additionally, a serial experiments to examine 3 key system performance and reliability characteristics are conducted, including command transmission time, transmission jitters, and rotation consistency. The analysis of these characteristics demonstrates the system’s potential and feasibility to be applicable in industry.
Bioinformatic analysis of endometrial miRNA expression profile at day 26–28 of pregnancy in the mare
Authors: Sadowska Agnieszka , Molcan Tomasz , Wójtowicz Anna , Lukasik Karolina , Pawlina-Tyszko Klaudia , Gurgul Artur , Ferreira-Dias Graca , Skarzynski Dariusz J. , Szóstek-Mioduchowska Anna 
Published: February 2024

AbstractThe establishment of the fetomaternal interface depends on precisely regulated communication between the conceptus and the uterine environment. Recent evidence suggests that microRNAs (miRNAs) may play an important role in embryo-maternal dialogue. This study aimed to determine the expression profile of endometrial miRNAs during days 26–28 of equine pregnancy. Additionally, the study aimed to predict target genes for differentially expressed miRNAs (DEmiRs) and their potential role in embryo attachment, adhesion, and implantation. Using next-generation sequencing, we identified 81 DEmiRs between equine endometrium during the pre-attachment period of pregnancy (day 26–28) and endometrium during the mid-luteal phase of the estrous cycle (day 10–12). The identified DEmiRs appear to have a significant role in regulating the expression of genes that influence cell fate and properties, as well as endometrial receptivity formation. These miRNAs include eca-miR-21, eca-miR-126-3p, eca-miR-145, eca-miR-451, eca-miR-491-5p, members of the miR-200 family, and the miRNA-17-92 cluster. The target genes predicted for the identified DEmiRs are associated with ion channel activity and sphingolipid metabolism. Furthermore, it was noted that the expression of mucin 1 and leukemia inhibitory factor, genes potentially regulated by the identified DEmiRs, was up-regulated at day 26–28 of pregnancy. This suggests that miRNAs may play a role in regulating specific genes to create a favorable uterine environment that is necessary for proper attachment, adhesion, and implantation of the embryo in mares.
Digital economy structuring for sustainable development: the role of blockchain and artificial intelligence in improving supply chain and reducing negative environmental impacts
Authors: Hong Zexin , Xiao Kun 
Published: February 2024

AbstractIn the current global context of environmental degradation and resource constraints, the pursuit of sustainable development has become an imperative. One avenue that holds promise for achieving this objective is the application of digital technologies, which have the potential to decouple economic growth from its carbon footprint. However, it is crucial to ensure that these technologies are designed and governed in a prudent manner, with a strong alignment to environmental priorities. This study focuses on exploring the potential roles of blockchain and artificial intelligence (AI) in supply chain coordination and impact mitigation. Furthermore, they have the capacity to incentivize recycling and circular business models, as well as facilitate carbon accounting and offsetting. To fully realize these benefits, it is essential to deploy these technologies within inclusive collaborative frameworks that take into consideration social and ecological considerations. The study also offers policy recommendations that highlight key leverage points for digital innovation, enabling countries to embark on smart and green industrial transformation pathways. By harnessing the potential of blockchain and AI in supply chains, governments can promote transparency, traceability, and accountability, thereby fostering sustainable practices and reducing environmental impacts. Incorporating blockchain and AI technologies into supply chain approaches leads to a substantial improvement in efficiency, as demonstrated by a numerical analysis. In conclusion, the integration of innovative digital technologies offers significant opportunities to optimize production systems and economic activity while prioritizing sustainability objectives for the betterment of society and the environment. These technologies have the potential to mitigate environmental externalities by addressing information imbalances within global supply chains. However, it is essential to prioritize inclusive governance that emphasizes democratic participation to mitigate any unintended negative consequences, especially for vulnerable communities. By ensuring inclusive decision-making processes, we can maximize the positive impact of these technologies while minimizing potential harm.
Therapeutic effect of knee extension exercise with single-joint hybrid assistive limb following total knee arthroplasty: a prospective, randomized controlled trial
Authors: Maeda Takaya , Sasaki Eiji , Kasai Takayuki , Igarashi Shigesato , Wakai Yuji , Sasaki Tomoyuki , Tsuda Eiichi , Ishibashi Yasuyuki 
Published: February 2024

AbstractThe single-joint hybrid assistive limb (HAL-SJ), an exoskeletal robotic suit, offers functional improvement. In this prospective randomized controlled trial, we investigated the therapeutic effects of knee extension exercises using the HAL-SJ after total knee arthroplasty (TKA). Seventy-six patients with knee osteoarthritis were randomly assigned to HAL-SJ or conventional physical therapy (CPT) groups. The HAL-SJ group underwent exercise using the HAL-SJ for 10 days postoperatively, in addition to CPT; the CPT group underwent only CPT. Pain intensity and active and passive knee extension angles were evaluated preoperatively and on postoperative days 1–10 and weeks 2 and 4. Performance tests and Knee Injury and Osteoarthritis Outcome Scores (KOOS) were evaluated preoperatively and at postoperative weeks 2 and 4. Statistical analysis showed that the HAL-SJ group significantly improved active and passive knee extension angles compared with the CPT group. The HAL-SJ group showed immediate improvement in active knee extension angle through day 5. There were no significant differences in results between the performance tests and KOOS. Knee extension exercises with the HAL-SJ improved knee pain and the angle of extension in the acute phase after TKA.
An efficient and accurate semi-analytical matching technique for waveguide-fed antennas
Authors: Negri Edoardo , Fuscaldo Walter , Tofani Silvia , Burghignoli Paolo , Galli Alessandro 
Published: February 2024

AbstractSeveral RF and microwave radiating devices, such as horn antennas, Fabry–Perot cavity antennas, and aperture-fed focusing devices, are excited through rectangular waveguides. The impedance matching of the overall system (from the waveguide feed to the radiating aperture) is a task of crucial importance that is often addressed by means of brute-force parameter-sweep full-wave analyses or blind optimization algorithms. In both cases, a significant amount of memory and time resources are required. For this purpose, we propose here a simple, yet effective solution, which only requires a single full-wave simulation and a semi-analytical procedure. The former is used to retrieve the antenna input impedance at the end of the waveguide port excitation. The semi-analytical procedure consists in a transmission-line equivalent circuit that models two waveguide discontinuities (namely two capacitive irises) within the waveguide section, whose position and geometric features are finely tuned to obtain a satisfactory impedance matching around the working frequency. The proposed method is shown to be effective in diverse and attractive application-oriented contexts, from the impedance matching of a Fabry–Perot cavity antenna to that of a wireless near-field link between two aperture-fed focusing devices. A remarkable agreement between full-wave simulations and numerical results is found in all cases. Thanks to its versatility, simplicity, and a rather low demand of computational resources, the proposed approach may become an essential tool for the effective design of waveguide-fed antennas.