bluuebunny/crossref_metadata_2025_split huggingface dataset:
DETERMINAN FIRM VALUE PERUSAHAAN PROPERTY DAN REAL ESTATE
Authors: Kurniawan Novan , Marhamah Marhamah - , Heridiansyah Jefri 
Published: October 2023

Abstract. Firm Value is an investor's perception of a company's level of success which is often linked to share prices. Firm value is investors' perception of a company which is reflected in its stable share price in the long term. Firm value is proxied by Price Book Value (PBV), which is the comparison between the share price and the book value per share. This research aims to examine the effect of profitability on firm value in property and real estate companies listed on the Indonesia Stock Exchange in 2020-2022.
The population in this research is 81 property and real estate companies listed on the Indonesia Stock Exchange (BEI) in 2020-2022. The sample in this study used the Purposive Sampling method so that a sample of 15 issuers was obtained. The variables in this research are Profitability, Leverage, Debt Policy, Liquidity, and Firm Value. The analytical method used in this research is multiple linear regression analysis.
Based on the results of data analysis, it is concluded that the profitability variable has no significant effect on Firm Value, the leverage variable has no significant effect on Firm Value, the debt policy variable has no significant effect on Firm Value, the liquidity variable has a significant effect on Firm Value.
For investors and potential investors, to look more carefully at market conditions and examine more closely the financial reports submitted by issuers (companies) before making investments, especially in relation to the amount of debt, in making debt policies, companies must pay more attention to liquidity and business risk factors.
PENGARUH STRATEGI PEMBELAJARAN KOOPERATIF TGT-PUZZLE TERHADAP KECERDASAN SPASIAL-VISUAL PADA MATERI BENTUK MOLEKUL
Authors: Ah. Fathul Jadid Anshori  , Kriesna Kharisma Purwanto  , Rendy Priyasmika  
Published: October 2023

Abstract: Chemistry is divided into two concepts, namely concrete and defined concepts. Geometry Molecul is one of the defined concepts because the idea is at the molecular level and is invisible to the eye. So, to understand the material molecular shape requires spatial-visual intelligence. Therefore, to determine the effect of the TGT-Puzzle cooperative learning strategy on spatial-visual intelligence on the geometry molecul, this study was conducted. The purpose of this study was to determine whether or not there was an increase in spatial-visual intelligence after being taught using the TGT-Puzzle cooperative learning strategy in class X IPA MA Hidayatul Ummah Pringgoboyo. This study uses an inferential statistical method using the paired sample t-test to determine whether or not there is an increase in visual-spatial intelligence after being taught using the TGT-Puzzle cooperative learning strategy. The analysis technique uses a paired sample t-test with a significance value (sig) < 0.05. The results of this study indicate an increase in spatial-visual intelligence scores after being taught using the TGT-Puzzle cooperative learning strategy, where if there is an increase in the category from average to high average or high average to superior.
Keywordsâ€‹: â€‹â€‹ Visual-spatial intelligence, TGT-Puzzle cooperative learning strategy, geometry molecul.
ANALISISIS KESALAHAN SISWA DALAM MENYELESAIKAN SOAL OPERASI ALJABAR MELALUI OBJEK LANGSUNG MATEMATIKA PADA SISWA KELAS VII SMP NEGERI 6 TONDANO
Authors: Niluh Norvi Natalia  , Rosiah J. Pulukadang  , Rosiah J. Pulukadang  , Anekke Pesik  , Derel F. Kaunang  
Published: October 2023

Abstract: This research is motivated by problems encountered in mathematics learning in SMP Negeri 6 Tondano in algebraic operating materials. This research aims to find out the mistakes made by students in solving mathematical problems, especially in algebraic operating materials related to direct objects of mathematics. This type of research is descriptive research. The population of this study was 20 students. The data collection techniques in this study use test methods, interviews, and documentation. The results of this study show that the mistakes that students often make in solving algebraic operations problems are related to facts, concepts, operations, and principles. (a) Of the fact errors included in the medium category i.e. 42.5% (b) Of the concept errors included in the high category i.e. 63.125% (c) Of the operating errors included in the high category i.e. 65.625% (d) Of the principle errors included in the medium high category 48,125%
Keywords: Error Analysis, Mathematical Direct Objects, Algebraic Operations
Implications of LDH in patients with coronavirus disease 2019 pneumonia
Authors: Mu Tong , Wang Xingguang , Lu Zhiming , Tong Jia 
Published: October 2023

ObjectiveThe objective of this study was to explore the value of serum lactic dehydrogenase (LDH) in the early diagnosis and prognostic evaluation of pneumonia associated with the novel coronavirus infection.MethodsA total of 101 patients with coronavirus disease 2019 (COVID-19) pneumonia were included in the study. According to the severity of the initial chest computed tomography (CT), the patients were divided into the ordinary pneumonia group and the severe pneumonia group and then divided into the remission group and the nonremission group according to the changes of the chest CT after medication treatment. The differences in general characteristics, underlying diseases, clinical symptoms, laboratory findings, and imaging examination outcomes between groups were observed retrospectively. To analyze the diagnostic performance of LDH, receiver operating characteristic (ROC) curves were constructed and the area under the curve (AUC) was calculated.ResultsCompared with ordinary pneumonia patients, patients in the severe group presented with significantly higher LDH, neutrophil count, high-sensitivity troponin T (HS-TnT), C-reactive protein (CRP), human serum amyloid A (SAA), N-terminal pro-brain natriuretic peptide (NTproBNP), and D-dimer. Compared with remission patients, non-remission patients presented with significantly higher LDH, neutrophil count, HS-TnT, CRP, SAA, procalcitonin (PCT), creatine kinase–MB mass (CKMB_M), NTproBNP, and D-dimer. In multivariate logistic regression analysis, we found that LDH [odds ratio (OR), 1.015; 95% confidence interval (CI), 1.006–1024; p = 0.001] and neutrophil count (OR, 1.352; 95% CI, 1.008–1.811; p = 0.044) were independently associated with exacerbation in COVID-19 patients. For ROC analysis, the AUC was 0.833 (95% CI, 0.729–0.936; p &lt; 0.001) when we use the LDH value of 256.69 U/L to discriminate the ordinary pneumonia and severe pneumonia patients. The AUC was 0.759 (95% CI, 0.603–0.914; p = 0.008) and the sensitivity is 92.3% when we combined the LDH (cutoff value 258.46 U/L) and the neutrophil count (cutoff value 6.76 × 109/L) to discriminate remission and non-remission patients.ConclusionThe level of LDH is associated with the severity of COVID-19 pneumonia and can be used as important indicators to evaluate the prognosis of patients.
Identification and analysis of differential miRNA–mRNA interactions in coronary heart disease: an experimental screening approach
Authors: Wang Jie , Liu Lanchun , Liu Chao , Cheng Nuo , Mao Qiyuan , Chen Cong , Hu Jun , He Haoqiang , Hui Xiaoshan , Qu Peirong , Lian Wenjing , Duan Lian , Dong Yan , Liu Yongmei , Li Jun 
Published: October 2023

ObjectiveThis aim of this study is to screen the differential molecules of kidney deficiency and blood stasis (KDBS) syndrome in coronary heart disease by high-throughput sequencing. In addition, the study aims to verify the alterations in the expression levels of miR-4685-3p and its regulated downstream, namely, C1QC, C4, and C5, using quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), and to determine whether the complement and coagulation cascade pathway is the specific pathogenic pathway.MethodsPatients diagnosed with unstable angina pectoris with KDBS syndrome, patients with non-kidney deficiency blood stasis (NKDBS) syndrome, and a Normal group were recruited. The clinical symptoms of each group were further analyzed. Illumina's NextSeq 2000 sequencing platform and FastQC software were used for RNA sequencing and quality control. DESeq software was used for differential gene expression (DGE) analysis. qPCR and ELISA verification were performed on DGE analysis.ResultsThe DGE profiles of 77 miRNA and 331 mRNA were selected. The GO enrichment analysis comprised 43 biological processes, 49 cell components, and 42 molecular functions. The KEGG enrichment results included 40 KEGG pathways. The PCR results showed that, compared with the Normal group, the miR-4685-3p levels decreased in the CHD_KDBS group (P = 0.001), and were found to be lower than those observed in the CHD_NKDBS group. The downstream mRNA C1 regulated by miR-4685-3p showed an increasing trend in the CHD_KDBS group, which was higher than that in the Normal group (P = 0.0019). The mRNA C4 and C5 in the CHD_KDBS group showed an upward trend, but the difference was not statistically significant. ELISA was utilized for the detection of proteins associated with the complement and coagulation cascade pathway. It was found that the expression level of C1 was significantly upregulated in the CHD_KDBS group compared with the Normal group (P &lt; 0.0001), which was seen to be higher than that in the CHD_NKDBS group (P &lt; 0.0001). The expression levels of C4 and C5 in the CHD_KDBS group were significantly lower than the Normal group, and were lower than that in the CHD_NKDBS group (P &lt; 0.0001).ConclusionThe occurrence of CHD_KDBS might be related to the activation of the complement and coagulation cascade pathway, which is demonstrated by the observed decrease in miR-4685-3p and the subsequent upregulation of its downstream C1QC. In addition, the expression levels of complement C4 and C5 were found to be decreased, which provided a research basis for the prevention and treatment of this disease.
Unraveling the role of VLDL in the relationship between type 2 diabetes and coronary atherosclerosis: a Mendelian randomization analysis
Authors: Feng Wenshuai , Guo Liuli , Liu Yiman , Ren Ming 
Published: October 2023

BackgroundThe causal link between Type 2 diabetes (T2D) and coronary atherosclerosis has been established through wet lab experiments; however, its analysis with Genome-wide association studies (GWAS) data remains unexplored. This study aims to validate this relationship using Mendelian randomization analysis and explore the potential mediation of VLDL in this mechanism.MethodsEmploying Mendelian randomization analysis, we investigated the causal connection between T2D and coronary atherosclerosis. We utilized GWAS summary statistics from European ancestry cohorts, comprising 23,363 coronary atherosclerosis patients and 195,429 controls, along with 32,469 T2D patients and 183,185 controls. VLDL levels, linked to SNPs, were considered as a potential mediating causal factor that might contribute to coronary atherosclerosis in the presence of T2D. We employed the inverse variance weighted (IVW), Egger regression (MR-Egger), weighted median, and weighted model methods for causal effect estimation. A leave-one-out sensitivity analysis was conducted to ensure robustness.ResultsOur Mendelian randomization analysis demonstrated a genetic association between T2D and an increased coronary atherosclerosis risk, with the IVW estimate at 1.13 [95% confidence interval (CI): 1.07–1.20]. Additionally, we observed a suggestive causal link between T2D and VLDL levels, as evidenced by the IVW estimate of 1.02 (95% CI: 0.98–1.07). Further supporting lipid involvement in coronary atherosclerosis pathogenesis, the IVW-Egger estimate was 1.30 (95% CI: 1.06–1.58).ConclusionIn conclusion, this study highlights the autonomous contributions of T2D and VLDL levels to coronary atherosclerosis development. T2D is linked to a 13.35% elevated risk of coronary atherosclerosis, and within T2D patients, VLDL concentration rises by 2.49%. Notably, each standard deviation increase in VLDL raises the likelihood of heart disease by 29.6%. This underscores the significant role of lipid regulation, particularly VLDL, as a mediating pathway in coronary atherosclerosis progression.
Role of inflammation and immunity in vascular calcification: a bibliometric and visual analysis, 2000–2022
Authors: Wang Chen , Liang Qingchun , He Siyi , Zhu Jie , Lin Xiafei , Lin Guanwen , Wu Duozhi , Zhang Wenqi , Wang Zhihua 
Published: October 2023

BackgroundIn recent years, a great deal of research has been done on vascular calcification (VC), and inflammation and immunity have been displayed to play important roles in the mechanism of VC. However, to date, no comprehensive or systematic bibliometric analyses have been conducted on this topic.MethodsArticles and reviews on the roles of inflammation and immunity in VC were obtained from the Web of Science Core Collection on August 5, 2022. Four scientometric software packages—HistCite, CiteSpace, VOSviewer, and R-bibliometrix—were used for the bibliometric and knowledge mapping analyses.ResultsThe obtained 1,868 papers were published in 627 academic journals by 9,595 authors of 2,217 institutions from 69 countries. The annual number of publications showed a clear growth trend. The USA and China were the most productive countries. Karolinska Institutet, Harvard University, and the University of Washington were the most active institutions. Stenvinkel P published the most articles, whereas Demer LL received the most citations. Atherosclerosis published the most papers, while Circulation was the most highly cited journal. The largest cluster among the 22 clusters, based on the analysis of co-citations, was osteo-/chondrogenic transdifferentiation. “Vascular calcification,” “inflammation,” “chronic kidney disease,” and “expression” were the main keywords in the field. The keyword “extracellular vesicle” attracted great attention in recent years with the strongest citation burst.ConclusionsOsteo-/chondrogenic transdifferentiation is the primary research topic in this field. Extracellular vesicles are expected to become a new research focus for exploring the inflammatory and immune mechanisms of VC.
Clinical characteristics of a large familial cohort with Medullary thyroid cancer and germline Cys618Arg RET mutation in an Israeli multicenter study
Authors: Rosenblum Rachel Chava , Hirsch Dania , Grozinsky-Glasberg Simona , Benbassat Carlos , Yoel Uri , Ishay Avraham , Zolotov Sagit , Bachar Gideon , Banne Ehud , Levy Sigal , Twito Orit 
Published: October 2023

ObjectiveTo determine genealogical, clinical and pathological characteristics of a cohort with Cys618Arg mutation from an Israeli multicenter MTC study.MethodsRetrospective database analysis examining RET mutations and comparing Cys618Arg and Cys634Arg/Thr/Tyr subgroups.ResultsGenetic testing was performed in 131/275 MTC patients (47.6%). RET mutations were found in 50/131 (38.2%), including Cys618Arg (28/50 cases,56%), and Cys634Arg/Thr/Tyr (15/50,30%). Through genealogical study, 31 MTC patients were found descendants of one family of Jewish Moroccan descent, accounting for 27/28 patients with documented Cys618Arg mutation and 4 patients without available genetic testing. Familial Cys618Arg cases (n=31) and Cys634Arg/Thr/Tyr cases (n=15, from 6 families) were compared. Although surgical age was similar (25.7 vs 31.3 years, p=0.19), the Cys618Arg group had smaller tumors (8.9mm vs 18.5mm, p=0.004) and lower calcitonin levels (33.9 vs 84.5 X/ULN, p=0.03). Youngest ages at MTC diagnosis were 8 and 3 years in Cys618Arg and Cys634Arg/Thr/Tyr cohorts, respectively. Long-term outcome was similar between groups. The Cys618Arg cohort had lower rates of pheochromocytoma (6.5% vs 53.3%, p=0.001) and primary hyperparathyroidism (3.2% vs 33.3%, p=0.01).ConclusionThis is the first description of RET mutation distribution in Israel. Of 131 tested MTC patients, Cys618Arg was the predominant mutation. To the best of our knowledge, this is the largest cohort of Cys618Arg mutation described. For Cys618Arg and Cys634Arg/Thr/Tyr cohorts, MTC was diagnosed earlier than expected, likely due to familial genetic screening, and MTC outcomes were similar between groups. International studies are necessary to further characterize the clinical features of Cys618 mutations due to their relative rarity.
RNA sequencing profiles reveals progressively reduced spermatogenesis with progression in adult cryptorchidism
Authors: Sun Weihao , Zhang Xinhui , Wang Lei , Ren Guanyu , Piao Shuguang , Yang Chenghua , Liu Zhiyong 
Published: October 2023

IntroductionThe fertility of cryptorchidism patients who didn’t perform corrective surgery will decrease with age. Herein, we elucidate the histological alterations and underlying molecular mechanism in patients with an increase in the disease duration from 20 to 40 years.MethodsTesticular tissues were obtained from three patients with cryptorchidism, ranging in age from 22 to 44 years. Three benign paracancerous testicular samples of matched ages were used as controls. The normal and undescended testicular tissues were stained with hematoxylin and eosin (HE) and immunofluorescence and all six testicular samples were subjected to RNA sequencing. RNA sequencing data were subjected to gene set enrichment analysis (GSEA), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI) network analysis, and Gene Ontology (GO) searches. Real-time reverse transcriptase polymerase chain reaction was used to confirm the DEGs.ResultsThe seminiferous tubules’ basement membrane thickens with age in healthy testes. As the period of cryptorchidism in the cryptorchid testis extended, the seminiferous tubules significantly atrophy, the number of spermatogenic cells declines, and the amount of interstitial fibrous tissue increases in comparison to normal tissues. The number of germ cells per cross-section of seminiferous tubules was significantly lower in cryptorchidism than in normal testicular tissues, according to immunofluorescence staining, but the number of Sertoli cells remained stable. RNA sequencing analysis identified 1150 differentially expressed genes (DEGs) between cryptorchidism and normal testicular tissues (fold change &gt;2 and p&lt;0.05), of which 61 genes were noticeably upregulated and 1089 were significantly downregulated. These genes were predominantly linked to sperm development and differentiation, and fertilization, according to GO analysis. Meiosis pathways were significantly downregulated in cryptorchidism, according to KEGG pathway analysis and GSEA (P&lt;0.001). PPI analysis was used to identify the top seven downregulated hub genes (PLCZ1, AKAP4, IZUMO1, SPAG6, CAPZA3, andROPN1L), which were then further verified by qPCR.DiscussionBy describing the histological changes and differential gene expression patterns in adult cryptorchid patients of different age groups, we discovered the progression mechanisms of undescended testes in adults with aging and identified seven significantly downregulated hub genes (PLCZ1, AKAP4, IZUMO1, SPAG6, CAPZA3, andROPN1L) in cryptorchid testis compared to normal testicular tissues. These genes played a role in the process of spermgenesis and are directly linked to the steady decline in fertility caused by cryptorchidism. Our study provided a better understanding of the molecular mechanisms underlying the loss of spermatogenesis in adult cryptorchidism, and give support for the development of adult cryptorchidism treatments.
Risk factor analysis and prediction model for papillary thyroid carcinoma with lymph node metastasis
Authors: Lu Juerong , Liao Jintang , Chen Yunhao , Li Jie , Huang Xinyue , Zhang Huajun , Zhang Bo 
Published: October 2023

ObjectiveWe aimed to identify the clinical factors associated with lymph node metastasis (LNM) based on ultrasound characteristics and clinical data, and develop a nomogram for personalized clinical decision-making.MethodsA retrospective analysis was performed on 252 patients with papillary thyroid carcinoma (PTC). The patient’s information was subjected to univariate and multivariate logistic regression analyses to identify risk factors. A nomogram to predict LNM was established combining the risk factors. The performance of the nomogram was evaluated using receiver operating characteristic (ROC) curve, calibration curve, cross-validation, decision curve analysis (DCA), and clinical impact curve.ResultsThere are significant differences between LNM and non-LNM groups in terms of age, sex, tumor size, hypoechoic halo around the nodule, thyroid capsule invasion, lymph node microcalcification, lymph node hyperechoic area, peak intensity of contrast (PI), and area under the curve (AUC) of the time intensity curve of contrast (P&lt;0.05). Age, sex, thyroid capsule invasion, lymph node microcalcification were independent predictors of LNM and were used to establish the predictive nomogram. The ROC was 0.800, with excellent discrimination and calibration. The predictive accuracy of 0.757 and the Kappa value was 0.508. The calibration curve, DCA and calibration curve demonstrated that the prediction model had excellent net benefits and clinical practicability.ConclusionAge, sex, thyroid capsule invasion, and lymph node microcalcification were identified as significant risk factors for predicting LNM in patients with PTC. The visualized nomogram model may assist clinicians in predicting the likelihood of LNM in patients with PTC prior to surgery.
The prognostic value of the histological shape of tumor negative sentinel nodes in breast cancer
Authors: Kooreman Loes F. S. , Dieleman Sabine , van Kuijk Sander M. J. , zur Hausen Axel , Smidt Marjolein L. , Grabsch Heike I. 
Published: October 2023

IntroductionSentinel lymph node (SLN) metastasis is an important predictor of prognosis in breast cancer (BC) patients, guiding treatment decisions. However, patients with the same BC subtype and tumor negative SLN (SLNneg) can have different survival outcomes. We hypothesized that the host anti-tumor immune reaction in SLNneg is important and results in morphometrically measurable changes in SLN size or shape which are related to patient prognosis.MethodsSurface area, circumference, long axis and short axis were histologically measured in 694 SLNneg from 356 cases of invasive BC and 67 ductal carcinoma in situ cases. The area occupied by fat was categorized as less or more than 50%. The long to short axis (L/S) ratio was calculated. The relationship between SLNneg morphometries and clinicopathological variables like tumor-infiltrating lymphocytes (TILs) within the primary tumor, as well as prognosis at 10 years follow up were analyzed.ResultsThe mean SLNneg surface area was 78.7mm2, circumference 40.3mm, long axis 13.1mm, short axis 8.2mm and L/S ratio 1.7. Larger surface area, long axis and short axis, including age &gt;55 years were associated with higher body mass index (BMI) and SLN fat over 50% (p&lt;0.003). In invasive BC, a high SLNneg L/S ratio (≥1.9) was related to poorer disease-free (HR=1.805, 95%CI 1.182-2.755, p=0.006) and overall (HR=2.389, 95%CI 1.481-3.851, p&lt;0.001) survival. A low SLNneg L/S ratio (&lt;1.9) was associated with high TILs in the primary BC (≥10%) (p=0.005). However a high TIL count was not of prognostic relevance.ConclusionsThis is the first study to suggest that morphometric characteristics of axillary SLNneg, like L/S ratio, could be used to predict prognosis in patients with SLNneg invasive BC of all subtypes. The association between low L/S ratio and high TILs suggest that SLN shape is related to immunological functioning of the SLN and could be used in addition to TIL evaluation. Regarding the dubious role of TILs in hormone receptor positive breast cancer, SLNneg morphometry to gain information about host immune status could especially be of benefit in this subtype. Further studies are warranted to better understand the underlying biological mechanisms.