bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining glycolysis and oxidative stress characterizations to assess prognosis in ovarian cancer
Authors: Huang Ying , Zeng Jianchang , Jiang Bingdong , Li Rong , Ma Hualing , Wang Xiufang , Yang Jun 
Published: November 2023

Abstract
Background
The research assessed glycolysis and oxidative stress scores as a potential indicator of prognosis in those afflicted with progressive ovarian cancer, which is known to have a poorer prognosis and is prone to platinum resistance.
Methods
The patients from the Ovarian Cancer (OV) dataset (TCGA-OV)is a test set. A total of 3 independent cohorts, GSE23554, GSE30587, and GSE14407 were utilized for outside verification. To discover glycolysis and oxidative stress-associated molecular patterns, unsupervised hierarchical clustering based on glycolysis and oxidative stress-associated genes was performed. Significant prognostic glycolysis and oxidative stress-associated genes were identified by LASSO(least absolute shrinkage and selection operator) regression analysis, as well as univariate and multivariate Cox regression. To differentiate between high-risk and low-risk categories, gene mutations, tumor immune microenvironments, and functional pathways were examined. Immunohistochemistry assays were utilized in this investigation to confirm the link between GLO1 and ovarian cancer prognosis.
Results
With 82 genes defined as gl ycolysis and oxidative stress- related genes ( GOSRGs ), the five GOSRGs (AKT1, ERBB2, GLO1, H6PD, and RB1) were identified to bulid a glycolysis and oxidative stress prognostic risk model. An analysis of the risk score via ROC curve revealed that the AUCs for 1, 3, and 5-years were 0.638, 0.588, and 0.635 respectively. The key genes are: GLO1, H6PD, and RB1. Glycolysis and oxidative stress-related pathways were discovered using GO, KEGG, and GSEA function analysis. The immune infiltration analysis revealed a statistically significant difference in 19 types of immune cells between the GOSs high and low groups. In addition, 15 genes were more prevalent in the GOSs high group. In univariate Cox regression analysis, GOSs, stage, and age are significantly related to prognosis. In nomogram analysis, the prognostic ability of age and stage on the model is higher than that of other variables.
Conclusions
The glycolysis-oxidative stress gene signature represents a promising tool for risk classification tool in OV patients.
WITHDRAWN: Adsorption kinetics of volatile organic compounds (VOCs) onto super activated renewable carbon (SARC) in low-carbon composites (LCCs)
Authors: Sun Bo , Sain Mohini 
Published: November 2023

Abstract

This is the first study of adsorption kinetics of volatile organic compounds (VOCs) onto super activated renewable carbon (SARC), with the aim of maximizing the removal efficiency of odor during the compounding of low-carbon composites (LCCs). Three different micrometer-sized SARC samples were prepared by alkali (NaOH) modification of renewable carbon, named as small sized SARC (S-SARC), medium sized SARC (M-SARC) and large sized SARC (L-SARC), respectively. The morphology and geometrical properties of these prepared SARC were investigated via scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) analysis, and their surface groups were determined by Boehm titration. Molecular dynamics (MD) simulations were applied to elucidate the migration behavior of SARC particles, with the aim of homogenizing the particles’ distribution and increasing their contact opportunities with VOCs molecules. The performance of SARC samples employed as adsorbents in LCCs had been exploited, and their adsorption capacity were evaluated by gas chromatography-mass spectroscopy (GC/MS) upon identifying and quantifying the volatile compounds. The Langmuir and Freundlich adsorption isotherms were applied, and the results revealed Freundlich was better fitting for the adsorption kinetics. The influence of various factors, e.g. chemical and physical properties of SARC, on the adsorption of VOCs were compared, and the results confirmed the dominant chemical adsorption (Freundlich Isotherm). Thus, using SARC, especially S-SARC can significantly promote the removal of odorous VOCs, and open up new horizons of full utilization of LCCs as substitutes for traditional high-carbon plastics in broader industries.
Cardiotoxicity: A Scoping Review
Authors: Talty Anna , Morris Roseanne , Deighan Carolyn 
Published: November 2023

Abstract
Background
Due to advancements in methods of cancer treatment, the population of people living with and beyond cancer is dramatically growing. The number of cancer survivors developing cardiovascular diseases and heart failure is also rising, due in part to the cardiotoxic nature of many cancer treatments. Guidelines are being increasingly released, emphasising the need for interdisciplinary action to address this gap in survivorship care. However, the extent to which interventions exist, incorporating the recommendations of cardio-oncology research, remains undetermined.
Objective
The aim of this scoping review is to assess the nature, extent and remit of existing cancer care interventions and their integration of cardio-oncology principles.
Methods
The review was conducted in accordance with the PRISMA Extension for Scoping Reviews Guidelines. Databases were independently searched for articles from 2010 to 2022, by two members of the research team. Data were charted and synthesised using the following criteria: (a) the focus of the intervention (b) the medium of delivery (c) the duration (d) the modalities included in the interventions (e) the research articles associated with each intervention (f) the type of studies conducted (g) key measures used (h) outcomes reported.
Results
Interventions encompassed six key modalities: Psychological Support, Physical Activity, Nutrition, Patient Education, Lifestyle and Caregiver Support. The focus, medium of delivery and duration of interventions varied significantly. While a considerable number of study protocols and pilot studies exist documenting HSMIs, only 25% appear to have progressed beyond this stage of development. Of those that have, the present review did not identify any ‘feasible’ interventions that covered each of the six modalities, while being generalisable to all cancer survivors and incorporating the recommendations from cardio-oncology research.
Conclusion
Despite the substantial volume of research and evidence from the field of cardio-oncology, the findings of this scoping review suggest that the recommendations from guidelines have yet to be successfully translated from theory to practice. There is an opportunity, if not necessity, for cardiac rehabilitation to expand to meet the needs of those living with and beyond cancer.
Anticancer activity features of imidazole-based ionic liquids and lysosomotropic detergents: in silico and in vitro studies
Authors: Gryniukova Anastasiia , Borysko Petro , Myziuk Iryna , Alieksieieva Diana , Hodyna Diana , Semenyuta Ivan , Kovalishyn Vasyl , Metelytsia Larysa , Rogalsky Sergiy , Tcherniuk Sergey 
Published: November 2023

Abstract
Long-chain imidazole-based ionic liquids (compounds 2, 4, 9) and lysosomotropic detergents (compounds 7, 3, 8) with potent anticancer activity were synthesized. Their inhibitory activities against neuroblastoma and leukaemia cell lines were predicted by the new in silico QSAR models. The cytotoxic activities of the synthesized imidazole derivatives were investigated on the SK-N-DZ (human neuroblastoma) and K-562 (human chronic myeloid leukaemia) cell lines. Compounds 2 and 7 showed the highest in vitro cytotoxic effect on both cancer cell lines. The docking procedure of compounds 2 and 7 into the NAD + coenzyme binding site of deacetylase sirtuin 1 (SIRT1) showed the formation of protein-ligand complexes with a calculated binding energy of -8.0 and − 8.1 kcal/mol, respectively. The interaction of SIRT1 with compounds 2, 7 and 9 and the interaction of bromodomain-containing protein 4 (BRD4) with compounds 7 and 9 were also demonstrated by thermal shift assay. Compounds 2, 4, 7, and 9 inhibited SIRT1 deacetylase activity in the SIRT-Glo assay. Compounds 7 and 9 showed a moderate inhibitory activity against aurora kinase A. In addition, compounds 3, 4, 8, and 9 inhibited the janus kinase 2 activity. The results obtained showed that long-chain imidazole derivatives exhibited cytotoxic activities on K562 leukaemia and SK-N-DZ neuroblastoma cell lines. Furthermore, these compounds inhibited a panel of molecular targets involved in leukaemia and neuroblastoma tumorigenesis. All these results suggest that both long-chain imidazole-based ionic liquids and lysosomotropic detergents may be an effective alternative for the treatment of neuroblastoma and chronic myeloid leukemia and merit further investigation.
A Window of Opportunity for ICU End-of-Life Care— A Retrospective Cohort Study
Authors: Darfelt Iben Strøm , Nielsen Anne Højager , Klepstad Pål , Neergaard Mette Asbjoern 
Published: November 2023

Abstract
PURPOSE
The 'window of opportunity' for ICU staff to deliver end-of-life (EOL) care lies in the timeframe from 'diagnosing the dying' to death. We aimed to describe the characteristics of trajectories for dying patients in Danish ICUs and to examine if physicians diagnose dying patients in time to perform EOL care and, if so, whether a window of opportunity for EOL care exists.
METHODS
From the Danish Intensive Care Database, we identified patients ≥18 years old admitted to a Danish ICU between January and December 2020 with an ICU stay of >96 h (four days) and who died during the ICU stay or within seven days after ICU discharge.
A chart review was performed on 250 consecutive patients admitted from January 1, 2020 in an ICU in the Central Denmark Region.
RESULTS
Most patients (223 (89%)) were diagnosed as dying. Of those patients who received mechanical ventilation, 171 (68%) died after abrupt discontinuation of mechanical ventilation and 63 (25%) died after gradual withdrawal. Patients whose mechanical ventilation was discontinued abruptly died after a median of one hour (IQR: 0-15) and five hours (IQR: 2-15) after a diagnosis of dying was recorded. In contrast, patients with a gradual withdrawal died after a median of 108 hours (IQR: 71-189) and 22 hours (IQR: 5-67) after a diagnosis of dying was recorded.
CONCLUSION
EOL care hinges on the ability to diagnose the dying. This study shows that there is a short window of opportunity for EOL care, particularly for patients being weaned from mechanical ventilation.
Early change in fatigue, insomnia, and cognitive impairment and symptom severity at 3-years post-treatment in breast cancer survivors
Authors: Lee Rachel M. , Rash Joshua A. , Seal Melanie , McCarthy Joy , Garland Sheila N. 
Published: November 2023

Abstract

Purpose
Breast cancer is the most common form of cancer among Canadian women. Survivorship challenges include fatigue, sleep disturbance, and cognitive impairment. This study examined: 1) symptom trajectory from diagnosis to three years; 2) whether symptom change in the first four months was associated with prolonged difficulties after three years; and 3) which factors were associated with deterioration in symptoms during the first four months.
Methods
This prospective observational cohort study examined 53 women (Mage = 58.6, 96.2% White, 67.9% Stage I) with newly diagnosed breast cancer over three years. Women completed assessments before starting treatment, four months, and three years after diagnosis. Three-way repeated-measures ANOVAs evaluated symptom trajectories. A repeated-measures mediation analysis was performed to determine if change from pre-treatment to four months accounted for change from pre-treatment to three years. A series of between-subjects ANOVAs were used to determine what variables significantly differed by deterioration status.
Results
Perceived cognitive impairment and fatigue increased linearly from diagnosis to three years. Change in fatigue in the first four months fully accounted for its change over three years. Insomnia severity and sleep quality deteriorated from diagnosis to four months, but returned to pre-treatment levels at three years. Those whose fatigue and cognitive ability deteriorated during the first four months were younger.
Conclusion
Efforts to identify those who are at risk of experiencing fatigue, sleep disturbance, and cognitive impairment; monitor patients early after receiving a diagnosis; and provide targeted interventions may prevent long-term deterioration and improve well-being.
Analysis of OCTA Features of the Retina and DTI Features of the Visual Pathway in Patients with Pituitary Adenoma
Authors: Tan Zhi , Liao Zhihui , Peng Shuying , Wen Xiujuan , Mo Wei , Xiao Keqi , Lai Xiaoying , Pang Yanhua 
Published: November 2023

Abstract
Purpose
The objective of this research is to investigate the ocular neural pathway in individuals affected by pituitary adenoma (PA), by combining two distinct methods: diffusion tensor imaging (DTI) and optical coherence tomography angiography (OCTA). The relationship between retinal blood flow density and neural fiber conduction function in these patients is explored.
Methods
The case group comprised 24 individuals who had been recently identified and pathologically verified as suffering from PA. A control group was assembled, consisting of 24 subjects who were aligned with the case group in terms of age and gender. All participants underwent OCTA, optical coherence tomography (OCT), and DTI examinations. Differences in the optic disc, macular OCTA parameters, OCT parameters, and DTI parameters between the two groups were compared, and correlations between these parameters were discussed.
Results
DTI parameters: In the case group, the optic nerve, optic chiasm, optic tract, and optic radiation showed a decline in their fractional anisotropy (FA) values when juxtaposed with the control group. Concurrently, the apparent diffusion coefficient (ADC) values for the optic nerve and optic radiation escalated in comparison to the control group. Parameters of OCTA: In the region of the macula, the density of blood flow across all layers, barring the deep capillary plexus (DCP), was found to be lesser in the subject group when contrasted with the reference group. There was a notable decline in the blood flow density in the radial peripapillary capillaries plexus (RPCP) and the superficial vascular complex (SVC) as well when set against the reference group. Correlation: A statistically significant correlation existed between the blood flow density of all layers of the macular area and the DTI parameters of the optic radiation. Importantly, strong associations were found between the superficial vascular network (SVN) in the macular area and the ADC measurement of the optic nerve, the FA measurement of the optic tract, and the ADC measurement of the optic radiation. This suggests that these values might serve as biomarkers for assessing neural fiber conduction function in the visual pathway.
Conclusion
OCTA can assess retinal blood flow density in PA patients, while DTI quantifies neural fiber conduction function. The combination of these techniques offers a novel approach for studying visual pathway injury in PA.
Co-culture of rice and crayfish increases the global warming potential-An evidence from soil culture with different water content
Authors: Liu Zhangyong , Zhang Dingyue , Hou Jun , Cao Yuxian , Hu Ren , Xiao Dakang 
Published: November 2023

Abstract
Rice–crayfish co-culture (RC) has been recognized as a comprehensive ecotype mode. Controversial opinions exist regarding the increase or decrease in global warming potential (GWP) by RC compared with rice monoculture (RM) because N2O and CH4 emission are affected by complex external factors including fertilizer, water management, and temperature (e.g., as in the RC system). However, whether soil from the RC field, except for external conditions, has different characteristics (i.e., organic N and carbon mineralization) that affect N2O and CH4 emission needs clarification. This study aimed to investigate the characteristics of soil organic carbon (SOC) and soil organic nitrogen (SON) mineralization and greenhouse gas emission in soils from RC (RC8 and RC15, indicating 8 and 15 years, respectively) and RM fields using the indoor biological culture method with different water-holding capacity (WHC) levels with a soil moisture content of 30%, 60%, and 90%s. Moreover, the dynamic model of SOC/SON mineralization was fitted to provide data support for fertilization and low-carbon management. RC soil had a higher rate of SOC/SON mineralization that mainly occurred in the first 7 days, and the mineralization rate increased first and then decreased with increasing WHC. The SOC mineralization rate constant (k) showed the trend of RC15 > RC8 > RM at 60% WHC, while the soil from these three fields had similar k values at 30% and 90% WHCs. Further, the half-revolution period of SOC (T1/2) was between 3.395 and 3.665, unlike the k value. The SON mineralization rate constant (k0) showed significant differences, with RC8 > RC15 > RM at 90% WHC, while no significant difference was found at 30% and 60% WHC and the k0 value varied from 0.103 to 0.120 under different WHC levels. RC8 and RC15 soils showed significantly higher soil net ammonification rate (0.5–3.04 g kg− 1 d− 1), net nitrification rate (0.04–0.09 g kg− 1 d− 1), and SON net mineralization rate (0.55–3.13 g kg− 1 d− 1) by more than 67%, 33%, and 65% compared with RM soil. At 30%, 60%, and 90% WHC, cumulative N2O emissions increased by 19–34%, 12–33%, and 17–32% in RC soil than in RM soil, respectively, and RC15 soil showed the highest emission. The cumulative CH4 emission significantly decreased by 23.1% and 11.5% at 30% WHC in RC8 and RC15 soils, respectively, than that of in RM soil. RC soil showed higher soil mineral N content and 31–59% higher GWP compared with those in RM soil, indicating that RC had higher mineral N support, while also having higher carbon emission risk. Therefore, N fertilizer can be reduced to a certain extent for long-time RC fields, and other effective low-carbon measures (e.g., enhanced-efficiency fertilizer, deep fertilization, and straw management) can be adopted to reduce GWP.
Remnant cholesterol and the risk of carotid plaque in hypertension: results from a community-based screening in Hangzhou, China
Authors: Yu Zhecong , Yang Haifeng , Shou Biqi , Cheng Zongxue , Jiang Caixia , Ye Yang , Xu Jue 
Published: November 2023

Abstract
Background
Elevated remnant cholesterol (RC) is considered a risk factor for atherosclerotic cardiovascular disease, but whether this association applies to the Chinese population with hypertension has not been found. We aimed to explore the association between RC levels and carotid plaque in patients with hypertension.
Methods
8523 hypertensive patients aged ≥ 60 years with serum lipids and carotid ultrasonography data were included in this community-based screening. Fasting RC was calculated as total cholesterol minus high-density lipoprotein cholesterol minus low-density lipoprotein cholesterol (LDLC). The associations of RC levels with carotid plaque risk were evaluated using Logistic regression and restricted cubic spline models.
Results
Carotid plaque was screened in 4821 (56.6%) subjects. After multivariable-adjusted, RC was significantly related to carotid plaque [Odd ratio (OR)] = 1.043 per 0.1 mmol/L increase, 95% confidence interval (CI): 1.031–1.056]. The highest versus lowest quartile of RC was 1.928 (1.673–2.223) for carotid plaque. A nonlinear association was found between serum RC levels and the risk of carotid plaque (P for nonlinearity < 0.001). Moreover, an RC > 0.78 mmol/L differentiated patients at a higher risk of carotid plaque compared to those at lower concentrations, regardless of whether LDLC was on target at 2.59 mmol/L.
Conclusion
In Chinese patients with hypertension, elevated RC was positively associated with carotid plaque, independent of LDLC and other conventional risk factors.
Therapeutic Effect of Anwulignan on Knee Osteoarthritis and Its Mechanism through Cell Pyroptosis Pathway in Rats
Authors: Niu Shengnan , Jiang Weihai , Zhao Chenghe , Chen Hongxu , Wang Chunmei , Sun Jinghui , Zhang Chengyi , Li He 
Published: November 2023

Abstract
Anwulignan (AN) is a monomer compound in the lignans of Schisandra chinensis. In this study, the effect of AN on knee osteoarthritis (KOA) in male SD rats was observed and its potential mechanisms were explored. The results showed that AN could significantly increase the number of standing times of rats within the same time, reduce the degree of knee joint swelling in rats, and alleviate the damage of cartilage tissue. AN could increase the activity of superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA) and reactive oxygen species (ROS) in the synovial tissue of rats, and reduce the levels of inflammatory factors such as tumor necrosis factor-α (TNF-α), interlukin (IL)-6, IL-1β and IL-18, prostaglandin E2 (PGE2) and nitric oxide (NO) in the serum and synovial tissue of rats. AN could play an antioxidant role by regulating the nuclear factor erythroid E2-related factor 2 (Nrf2) signaling pathway-related proteins, and play anti-inflammatory and anti-pyroptosis roles by regulating pyroptosis pathway-related proteins, including the nuclear factor-k-gene binding (NF-κB) signaling pathway-related proteins and NOD-like receptor family pyrin domain containing 3 (NLRP3). AN could significantly increase the expression level of CollagenII protein in KOA rats and decrease the expression levels of matrix metalloproteinase1 (MMP1) and matrix metalloproteinase13 (MMP13) proteins. The results of immunofluorescence analysis further confirmed the protective effect of AN against KOA in rats, which may be related to the regulation of Nrf2/HO-1 signaling pathway to play an antioxidant role and the regulation of TLR4/NLRP3/Caspase-1 signaling pathway-mediated anti-pyroptosis effect.
Changes in Ruminoreticular Temperature and Body Activity After Akabane Vaccination in Pregnant Hanwoo (Bos taurus coreanae)
Authors: Yi Junkoo , Gim Gyong-Min , Ha Jaejung , Park Jinyeon , Yu Daejung , Kwon Woo-Sung , Kim Daehyun 
Published: November 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Geospatial Information systems for modeling accessibility to service points in Zimbabwe with reference to health provision centers
Authors: MAZHAWIDZA THERESA , MURWIRA K S , SHEKEDE M D 
Published: November 2023

Abstract
Objectives Understanding geographical accessibility to services is important for informed decision making and policy formulation pertaining to infrastructural development and sustainable disaster management. The aim of this study was to develop an interactive travel time tool that can be used to model accessibility to any destination in Zimbabwe.
Setting We modelled physical accessibility to the health facility network in Zimbabwe using time as a metric measure. The research takes into consideration the various factors that influence the speed of traversing the landscape across different landcover types and terrain-conditions. Modelling was done using SAGA GIS and High Speed Computing.
Results This research was applied specifically on health service centres as destinations, and the model output revealed how certain areas with rugged terrain or dense vegetation and impassable streams can cause a set of villages to be “cut off” from apparently close health facilities as the bird flies.
Conclusions The study successfully demonstrates the application of GIS in accessibility modelling and reflects opportunities for integrating accessibility modelling with service provision and infrastructural planning.