bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA Interference of Soybean Isoflavone Synthase Genes Leads to Silencing in Tissues Distal to the Transformation Site and to Enhanced Susceptibility to Phytophthora sojae
Authors: Subramanian Senthil , Graham Madge Y. , Yu Oliver , Graham Terrence L. 
Published: March 2005

Abstract
Isoflavones are thought to play diverse roles in plant-microbe interactions and are also potentially important to human nutrition and medicine. Isoflavone synthase (IFS) is a key enzyme for the formation of the isoflavones. Here, we examined the consequences of RNAi silencing of genes for this enzyme in soybean (Glycine max). Soybean cotyledon tissues were transformed with Agrobacterium rhizogenes carrying an RNAi silencing construct designed to silence expression of both copies of IFS genes. Approximately 50% of emerging roots were transformed with the RNAi construct, and most transformed roots exhibited &gt;95% silencing of isoflavone accumulation. Silencing of IFS was also demonstrated throughout the entire cotyledon (in tissues distal to the transformation site) both by high-performance liquid chromatography analysis of isoflavones and by real-time reverse transcription-PCR. This distal silencing led to a nearly complete suppression of mRNA accumulation for both the IFS1 and IFS2 genes and of isoflavone accumulations induced by wounding or treatment with the cell wall glucan elicitor from Phytophthora sojae. Preformed isoflavone conjugates were not reduced in distal tissues, suggesting little turnover of these stored isoflavone pools. Distal silencing was established within just 5 d of transformation and was highly efficient for a 3- to 4-d period, after which it was no longer apparent in most experiments. Silencing of IFS was effective in at least two genotypes and led to enhanced susceptibility to P. sojae, disrupting both R gene-mediated resistance in roots and nonrace-specific resistance in cotyledon tissues. The soybean cotyledon system, already a model system for defense signal-response and cell-to-cell signaling, may provide a convenient and effective system for functional analysis of plant genes through gene silencing.
Evaluation and Transferability of the Noah Land Surface Model in Semiarid Environments
Authors: Hogue Terri S. , Bastidas Luis , Gupta Hoshin , Sorooshian Soroosh , Mitchell Ken , Emmerich William 
Published: March 2005

Abstract
This paper investigates the performance of the National Centers for Environmental Prediction (NCEP) Noah land surface model at two semiarid sites in southern Arizona. The goal is to evaluate the transferability of calibrated parameters (i.e., direct application of a parameter set to a “similar” site) between the sites and to analyze model performance under the various climatic conditions that can occur in this region. A multicriteria, systematic evaluation scheme is developed to meet these goals. Results indicate that the Noah model is able to simulate sensible heat, ground heat, and ground temperature observations with a high degree of accuracy, using the optimized parameter sets. However, there is a large influx of moist air into Arizona during the monsoon period, and significant latent heat flux errors are observed in model simulations during these periods. The use of proxy site parameters (transferred parameter set), as well as traditional default parameters, results in diminished model performance when compared to a set of parameters calibrated specifically to the flux sites. Also, using a parameter set obtained from a longer-time-frame calibration (i.e., a 4-yr period) results in decreased model performance during nonstationary, short-term climatic events, such as a monsoon or El Niño. Although these results are specific to the sites in Arizona, it is hypothesized that these results may hold true for other case studies. In general, there is still the opportunity for improvement in the representation of physical processes in land surface models for semiarid regions. The hope is that rigorous model evaluation, such as that put forth in this analysis, and studies such as the Project for the Intercomparison of Land-Surface Processes (PILPS) San Pedro–Sevilleta, will lead to advances in model development, as well as parameter estimation and transferability, for use in long-term climate and regional environmental studies.
Desmoplastic small round cell tumour : Cytological and immunocytochemical features
Authors: Granja Nara M , Begnami Maria D , Bortolan Jeni , Filho Adhemar L , Schmitt Fernando C 
Published: March 2005

Background:
Desmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive neoplasm. The cytological diagnosis of these tumors can be difficult because they show morphological features quite similar to other small round blue cells tumors. We described four cases of DSRCT with cytological sampling: one obtained by fine needle aspiration biopsy (FNAB) and three from serous effusions. The corresponding immunocytochemical panel was also reviewed.

Methods:
Papanicolaou stained samples from FNAB and effusions were morphologically described. Immunoreaction with WT1 antibody was performed in all cytological samples. An immunohistochemical panel including the following antibodies was performed in the corresponding biopsies: 34BE12, AE1/AE3, Chromogranin A, CK20, CK7, CK8, Desmin, EMA, NSE, Vimentin and WT1.

Results:
The smears showed high cellularity with minor size alteration. Nuclei were round to oval, some of them with inconspicuous nucleoli. Tumor cells are clustered, showing rosette-like feature. Tumor cells in effusions and FNA were positive to WT1 in 3 of 4 cytology specimens (2 out 3 effusions and one FNA). Immunohistochemical reactions for vimentin, NSE, AE1/AE3 and WT1 were positive in all cases in tissue sections.

Conclusion:
The use of an adjunct immunocytochemical panel coupled with the cytomorphological characteristics allows the diagnosis of DSRCT in cytological specimens.
Effect of humic acids on toxicity of DDT and chlorpyrifos to freshwater and estuarine invertebrates
Authors: Mézin Laurent C. , Hale Robert C. 
Published: March 2005

Abstract
The effects of dissolved humic acids(HA)on the acute toxicities of the organophosphate pesticide chlorpyrifos and the organochlorine pesticide 4,4′-dichlorodiphenyltrichloroethane (DDT) were assessed by using freshwater crustaceans (Cerio-daphnia dubia) and saltwater crustaceans (Americamysis bahia). The effects of filtered Aldrich HAs (10–100 mg/L) on organism mortality were determined. Humic acids had no effect on mortality of A. bahia for either pesticide at a salinity of 20%, but greatly reduced the mortality of C. dubia for both pesticides in freshwater (0%). In the latter case, the effect was proportional to the HA concentration. The difference in toxicity mitigation as a function of salinity is believed to be due to conformational changes in the HA molecules, which impact pesticide—HA binding, rather than to organismal effects.
Biomarker responses and chemical analyses in fish indicate leakage of polycyclic aromatic hydrocarbons and other compounds from car tire rubber
Authors: Stephensen Eiríkur , Adolfsson-Erici Margaretha , Celander Malin , Hulander Mats , Parkkonen Jari , Hegelund Tove , Sturve Joachim , Hasselberg Linda , Bengtsson Madeleine , Förlin Lars 
Published: March 2005

Abstract
Rubber tire material contains toxic compounds including oils rich in polycyclic aromatic hydrocarbons (PAH), so-called highly aromatic (HA) oils, as well as other reactive additives used as antioxidants, antiozonants, and vulcanization accelerators. The toxicity of rubber tire leachates to aquatic organisms has been demonstrated before. However, previous studies have focused on lethal rather than sublethal effects. We kept rainbow trout (Oncorhynchus mykiss) in tanks with two types of tires: a tire containing HA oils in the tread or a tire free of HA oils in the tread. After 1 d of exposure, an induction of cytochrome P4501A1 (CYP1A1) was evident in both exposed groups, measured as elevated ethoxyresorufin-O-deethylase (EROD) activity and increased CYP1A1 mRNA levels. After two weeks of exposure, EROD activity and CYP1A1 mRNA were still high in fish exposed to leachate from HA oil-containing tire, whereas the effect was somewhat lower in fish exposed to leachate from HA oil-free tread tire. Compounds in the tire leachates also affected antioxidant parameters. Total glutathione concentration in liver as well as hepatic glutathione reductase, glutathione S-transferase, and glucose-6-phosphate dehydrogenase activities were markedly elevated after two weeks of exposure in both groups. The responses were greater in the group exposed to leachate from HA oil-free tread tire. Vitellogenin measurements did not indicate leakage of estrogenic compounds from the tires. Chemical analyses of bile from exposed fish revealed the presence of hydroxylated PAH as well as aromatic nitrogen compounds indicating uptake of these compounds by the fish.
Occurrence of neutral and acidic drugs in the effluents of Canadian sewage treatment plants
Authors: Metcalfe Chris D. , Koenig Brenda G. , Bennie Don T. , Servos Mark , Ternes Thomas A. , Hirsch Roman 
Published: March 2005

Abstract
Samples of influent (untreated) and effluent (treated) from 18 sewage treatment plants (STPs) in 14 municipalities in Canada were analyzed for residues of selected prescription and nonprescription drugs. Several neutral and acidic drugs were detected in effluents, including analgesic/anti-inflammatory agents, lipid regulators, and an antiepileptic drug, carbamazepine. Residues were extracted from effluents by solid-phase extraction, followed by either methylation and analysis of acidic drugs by gas chromatography/mass spectrometry or direct analysis of neutral drugs by liquid chromatography/tandem mass spectrometry. Analgesic/anti-inflammatory drugs such as ibuprofen and naproxen, as well as the metabolite of acetylsalicyclic acid, salicylic acid, were often detected in final effluents at μg/L concentrations. The acidic lipid regulator, clofibric acid, and the analgesic/anti-inflammatory drug diclofenac were not detected in any final effluent samples, which is not consistent with data from Europe. The precursor to clofibric acid, clofibrate, is not widely prescribed as a lipid regulator in Canada. However, the lipid regulators bezafibrate and gemfibrozil were detected in some samples of influent and effluent. The chemotherapy drugs ifosfamide and cyclophosphamide and the anti-inflammatory phenazone were not detected in influent or effluent samples, but the vasodilator drug pentoxyfylline was detected at ng/L concentrations in some final effluents. The widespread occurrence of carbamazepine at concentrations as high as 2.3 μg/L may be explained by use of this drug for other therapeutic purposes besides treatment of epilepsy and its resistance to elimination in STPs. The rates of elimination of ibuprofen and naproxen appeared to be elevated in STPs with hydraulic retention times for sewage greater than 12 h.
Issues of replicability in Monte Carlo modeling: A case study with a pesticide leaching model
Authors: Dubus Igor G. , Janssen Peter H. M. 
Published: March 2005

Abstract
Sensitivity and uncertainty analyses based on Monte Carlo sampling were undertaken for various numbers of runs of the pesticide leaching model (PELMO). Analyses were repeated 10 times with different seed numbers. The ranking of PELMO input parameters according to their influence on predictions for leaching was stable for the most influential parameters. For less influential parameters, the sensitivity ranking was severely influenced by the seed number used. For uncertainty analyses, probabilities of exceeding a particular concentration were significantly influenced by the seed number used in the random sampling of values for the two parameters considered, even for those cases in which 5,000 model runs were undertaken (coefficient of variation of 10 replicated analyses, 5%). A decrease in the variability of exceedance probabilities could be achieved by further increasing the number of model runs. However, this may prove to be impractical when complex deterministic models with a relatively long running time are used. Attention should be paid to replicability aspects by modelers when devising their approach to assessing the uncertainty associated with the modeling and by decision makers when examining the results of probabilistic approaches.
The effects of low hardness and pH on copper toxicity to Daphnia magna
Authors: Long Kristen E. , Van Genderen Eric J. , Klaine Stephen J. 
Published: March 2005

Abstract
The majority of metal toxicity data available for freshwater organisms have been generated in laboratory water at pH &gt; 6.5 and hardness &gt; 50 mg/L as CaCO3. Extrapolation of these results to soft surface waters (i.e., hardness ≤ 40 mg/L as CaCO3), similar to predominant conditions in the southeastern United States, may prove challenging. For example, South Carolina has surface waters that average 20 mg/L as CaCO3, and exist at extremes of 1 and 600 mg/L as CaCO3. This research characterized the acute toxicity of Cu to Daphnia magna in waters with low hardness and low pH. The 48-h total Cu median lethal concentrations were related to water hardness over a hardness range of 8 to 51 mg/L as CaCO3. Although toxicological differences existed between water hardness of 7 and 20 mg/L as CaCO3 (p = 0.0001), differences in pH (range 5.5–8.5) did not influence acute Cu toxicity. Results of these laboratory studies will provide the data needed to more accurately predict organism response to Cu in waters with low pH and low hardness.
The effect of counterion and percolation on the toxicity of lead for the springtail Folsomia candida in soil
Authors: Bongers Marina , Rusch Ben , van Gestel Cornelis A. M. 
Published: March 2005

Abstract
In standard soil toxicity tests, heavy metals are amended as water-soluble salts. The role of the counterion in metal salt toxicity is scarcely looked into. In this study, we assessed the contribution of nitrate and chloride to the toxicity of lead to Folsomia candida in a natural standard soil. Both lead salts were tested according the standard test protocol as well as after percolation of the soil with deionized water. Lead nitrate was more toxic than lead chloride for survival as well as reproduction. Percolation proved to be an effective method to remove counterions from the soil. Survival of F. candida increased for both metal salts when percolation was included. Percolation reduced the reproduction toxicity of lead, the effect of which was largest for the nitrate salt. In percolated treatments, the nitrate and chloride lead salts did not differ in toxicity. It is concluded that counterions contribute to metal toxicity and that nitrate is more toxic to F. candida than chloride.
Effect of prairie grass on the dissipation, movement, and bioavailability of selected herbicides in prepared soil columns
Authors: Belden Jason B. , Phillips Todd A. , Coats Joel R. 
Published: March 2005

Abstract
Phytoremediation of pesticide-contaminated sites using a prairie grass mixture (big bluestem, yellow indiangrass, and switch grass) has been suggested as a low-cost in situ remediation strategy. In this study, the proposed phytoremediation technique was applied to artificially prepared soil columns that were fortified with high concentrations of four herbicides (atrazine, alachlor, metolachlor, and pendimethalin). The fate and toxicity of the herbicides were compared with results from soil columns lacking vegetation. After either 150 or 240 d of phytoremediation, soils were watered with 7.5 cm of water, and leachate was collected. Columns were then divided into three sections (top, middle, bottom). For each section of the column, chemical analysis (ethyl acetate and water extractions), earthworm accumulation tests, and lettuce seedling growth tests were performed. The leachate was chemically analyzed and tested for chronic toxicity to algae. Atrazine and alachlor degraded rapidly in the column, and the total amount recoverable was less than 2% of applied. After 250 d, vegetation reduced the total recoverable amounts of metolachlor and pendimethalin by 78 and 39%, respectively. Metolachlor was the only compound found in leachate, and the amounts recovered were reduced 5- to 20-fold by vegetation. Vegetation decreased the bioavailability of pendimethalin as measured by 8-d, earthworm bioaccumulation factors (BAFs) and lettuce seedling growth assays. Decreases in mobility and bioavailability indicate that this technique may stabilize pesticide residues in addition to increasing dissipation rates.
Relative sensitivity distribution of aquatic invertebrates to organic and metal compounds
Authors: von der Ohe Peter Carsten , Liess Matthias 
Published: March 2005

Abstract
In the field, a multitude of species can be exposed to numerous toxicants; thus, the sensitivity of individual species to particular toxicants must be known to predict effects and to analyze changes in species composition. For most species, no information about their toxicant sensitivity is available. To address this limitation, we have grouped the available information to assign sensitivities to aquatic invertebrate taxa relative to Daphnia magna. With respect to organic compounds, most taxa of the orders Anisoptera, Basommatophora, Coleoptera, Decapoda, Diptera, Ephemeroptera, Eulamellibranchiata, Heteroptera, Hirudinea, Isopoda, Oligochaeta, Prosobranchia, Trichoptera, Tricladida, and Zygoptera are less sensitive than D. magna. Some taxa of the Amphipoda, Plecoptera, and Cladocera (other than D. magna) are significantly more sensitive. For organic compounds, approximately 22% of the investigated taxa were more sensitive than D. magna. Most taxa of the orders Amphipoda, Basommatophora, Diptera, Ephemeroptera, Eulamellibranchiata, Heteroptera, Isopoda, Oligochaeta, and Tricladida are significantly less sensitive than D. magna to metal compounds. The taxa belonging to the Crustacea, with the exception of the order Isopoda, are much more sensitive. For metal compounds, approximately 30% of the investigated taxa were more sensitive than D. magna. Hence, D. magna is among the most sensitive taxa regarding both groups of toxicants. The sensitivities for several taxa are listed, and use of the relative sensitivity distribution to link toxicant effects in mesocosm studies and field investigations is discussed.
Estrogenic compounds affect development of harpacticoid copepod Tigriopus japonicus
Authors: Marcial Helen S. , Hagiwara Atsushi , Snell Terry W. 
Published: March 2005

Abstract
The aim of this investigation was to evaluate the impact of estrogenic compounds onthe harpacticoid copepod Tigriopus japonicus after continuous exposure to environmentally relevant concentrations. Natural estrogen (17β-estradiol), three known estrogenic compounds in vertebrates (bisphenol A, 4-nonylphenol, p-t-octylphenol), and an invertebrate molting hormone (20-hydroxyecdysone) were tested for their effects on development and reproductive characters in two successive generations of T. japonicus. Less than 24-h-old nauplii (parentals) were exposed to four sublethal concentrations of these compounds for 21 d at 25°C. The first brood of nauplii (F1) produced was monitored further under the same culture conditions and exposures to test compounds. Results showed that all estrogenic compounds affected development (both in number of days to reach copepodid stage and sexual maturity) in the parental generation. Similar effects were apparent in the F1; however, fecundity, sex ratio, and survival were not significantly affected, even at concentrations as high as 10 μg/L (nominal concentration). The invertebrate molting hormone 20-hyroxyecdysone had no detectable effect on any of the endpoints tested but gave the lowest 48-h 50% lethal concentration (LC50) value. The results suggest that endocrine disruption could occur in copepods following exposure to environmentally relevant concentrations of estrogenic compounds, especially if they are exposed starting from embryonic development.
Derivation of a chronic site-specific water quality standard for selenium in the Great Salt Lake, Utah, USA
Authors: Brix Kevin V. , Deforest David K. , Cardwell Rick D. , Adams William J. 
Published: March 2005

Abstract
The purpose of this study was to develop a site-specific water quality standard for selenium in the Great Salt Lake, Utah, USA. The study examined the bioavailability and toxicity of selenium, as selenate, to biota resident to the Great Salt Lake and the potential for dietary selenium exposure to aquatic dependent birds that might consume resident biota. Because of its high salinity, the lake has limited biological diversity with bacteria, algae, diatoms, brine shrimp, and brine flies being the only organisms present in the main (hypersaline) portions of the lake. To evaluate their sensitivity to selenium, a series of acute and chronic toxicity studies were conducted on brine shrimp (Artemia franiciscana), brine fly (Ephydra cinerea), and a hypersaline alga (Dunaliella viridis). The resulting acute and chronic toxicity data indicated that resident species are more selenium tolerant than many freshwater species. Because sulfate is known to reduce selenate bioavailability, this selenium tolerance is thought to result in part from the lake's high ambient sulfate concentrations (&gt;5,800 mg/L). The acute and chronic test results were compared to selenium concentrations expected to occur in a mining effluent discharge located at the south end of the lake. Based on these comparisons, no appreciable risks to resident aquatic biota were projected. Field and laboratory data collected on selenium bioaccumulation in brine shrimp demonstrated a linear relationship between water and tissue selenium concentrations. Applying a dietary selenium threshold of 5 mg/kg dry weight for aquatic birds to this relationship resulted in an estimate of 27 μg/L Se in water as a safe concentration for this exposure pathway and an appropriate chronic site-specific water quality standard. Consequently, protection of aquatic birds represents the driving factor in determining a site-specific water quality standard for selenium.
Behavioral response of young rainbow trout (Oncorhynchus mykiss) to forest fire-retardant chemicals in the laboratory
Authors: Wells Jason B. , Little Edward E. , Calfee Robin D. 
Published: March 2005

Abstract
Fire-retardant chemicals often are applied in relatively pristine and environmentally sensitive areas that are potentially inhabited by endangered or threatened aquatic species. Avoidance of contaminants is an adaptive behavior that may reduce exposure to harmful conditions. We evaluated the avoidance responses of rainbow trout (Oncorhynchus mykiss) to concentrations of fire-retardant chemicals and alternate constituent formulations ranging from 0.65 to 26 mg/L. Countercurrent avoidance chambers were used in a flow-through design with receiving water at each end and a drain at the center to create a distinct boundary between treatment water and reference water. Rainbow trout consistently avoided water treated with retardants at all concentrations tested. The magnitude of the avoidance response did not appear to follow a concentration-response relationship, but rather was an all-or-none response.