bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eviscerated liver: an extremely rare complication of abdominal wound dehiscense through a midline incision
Authors: Elbashier Mohanad , Rafei Ahmed , Abdulkarim Abdulwahab , Soud Mohamed , Musa Hassan , Taher Ali , Suliman Alsadig 
Published: November 2023

Abstract
Abdominal wound dehiscense, or burst abdomen, is a critical postoperative complication necessitating immediate intervention. We present an extremely rare case of left hepatic lobe evisceration through wound dehiscense in a 65-year-old female receiving palliative care for hypopharyngeal squamous cell carcinoma. The patient’s midline incision that was performed for feeding jejunostomy tube displayed liver protrusion on Day 14 postoperatively. Surgical exploration revealed a healthy liver, prompting reduction and secondary sutures to prevent complications. Abdominal wound dehiscense risk factors, including advanced age, poor nutrition, and medical illness, contribute to its occurrence. Although guidelines for liver evisceration management are lacking, our case emphasizes proper technique, wound care, and nutritional support to aid the healing process and to ensure a better outcome for the patients.
Persulfidation of plant and bacteroid proteins is involved in legume nodule development and senescence
Authors: Matamoros Manuel A , Romero Luis C , Tian Tao , Román Ángela , Duanmu Deqiang , Becana Manuel 
Published: November 2023

Abstract
Legumes establish symbiosis with rhizobia, forming nitrogen-fixing nodules. The central role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in nodule biology has been clearly established. Recently, hydrogen sulfide (H2S) and other reactive sulfur species (RSS) have emerged as novel signaling molecules in animals and plants. A major mechanism by which ROS, RNS, and RSS fulfil their signaling role is the post-translational modification of proteins. To identify possible functions of H2S in nodule development and senescence, we used the tag-switch method to quantify changes in the persulfidation profile of common bean (Phaseolus vulgaris) nodules at different developmental stages. Proteomic analyses indicate that persulfidation plays a regulatory role in plant and bacteroid metabolism and senescence. The effect of a H2S donor on nodule functioning and on several proteins involved in ROS and RNS homeostasis was also investigated. Our results using recombinant proteins and nodulated plants support a crosstalk among H2S, ROS, and RNS, a protective function of persulfidation on redox-sensitive enzymes, and a beneficial effect of H2S on symbiotic nitrogen fixation. We conclude that the general decrease of persulfidation levels observed in plant proteins of aging nodules is one of the mechanisms that disrupt redox homeostasis leading to senescence.
Leaf abaxial immunity to powdery mildew in Arabidopsis is conferred by multiple defense mechanisms
Authors: Wu Ying , Sexton W Kyle , Zhang Qiong , Bloodgood David , Wu Yan , Hooks Caroline , Coker Frank , Vasquez Andrea , Wei Cheng-I , Xiao Shunyuan 
Published: November 2023

Abstract
Powdery mildew fungi are obligate biotrophic pathogens that only invade plant epidermal cells. There are two epidermal surfaces in every plant leaf: the adaxial (upper) side and the abaxial (lower) side. While both leaf surfaces can be susceptible to adapted powdery mildew fungi in many plant species, there have been observations of leaf abaxial immunity in some plant species including Arabidopsis. The genetic basis of such leaf abaxial immunity remains unknown. In this study, we tested a series of Arabidopsis mutants defective in one or more known defense pathways with the adapted powdery mildew isolate Golovinomyces cichoracearum UCSC1. We found that leaf abaxial immunity was significantly compromised in mutants impaired for both the EDS1/PAD4- and PEN2/PEN3-dependent defenses. Consistently, expression of EDS1–yellow fluorescent protein and PEN2–green fluorescent protein fusions from their respective native promoters in the respective eds1-2 and pen2-1 mutant backgrounds was higher in the abaxial epidermal cells than in the adaxial epidermal cells. Altogether, our results indicate that leaf abaxial immunity against powdery mildew in Arabidopsis is at least partially due to enhanced EDS1/PAD4- and PEN2/PEN3-dependent defenses. Such transcriptionally pre-programmed defense mechanisms may underlie leaf abaxial immunity in other plant species such as hemp and may be exploited for engineering adaxial immunity against powdery mildew fungi in crop plants.
A group VIIIa ethylene-responsive factor, CmERF4, negatively regulates waterlogging tolerance in chrysanthemum
Authors: Li Chuanwei , Wang Likai , Su Jiangshuo , Li Wenjie , Tang Yun , Zhao Nan , Lou La , Ou Xiaoli , Jia Diwen , Jiang Jiafu , Chen Sumei , Chen Fadi 
Published: November 2023

Abstract
Ethylene-responsive factors (ERF) play an important role in plant responses to waterlogging stress. However, the function and mechanism of action of ERFVIII in response to waterlogging stress remain poorly understood. In this study, we found that expression of the ERF VIIIa gene CmERF4 in chrysanthemum was induced by waterlogging stress. CmERF4 localized to the nucleus when expressed in tobacco leaves. Yeast two-hybrid and luciferase assays showed that CmERF4 is a transcriptional inhibitor. CmERF4 overexpression in chrysanthemum reduced plant waterlogging tolerance, whereas overexpression of the chimeric activator CmERF4-VP64 reversed its transcriptional activity, promoting higher waterlogging tolerance than that observed in wild-type plants, indicating that CmERF4 negatively regulates waterlogging tolerance. Transcriptome profiling showed that energy metabolism and reactive oxygen species (ROS) pathway-associated genes were differentially expressed between CmERF4-VP64 and wild-type plants. RT–qPCR analysis of selected energy metabolism and reactive oxygen species-related genes showed that the gene expression patterns were consistent with the expression levels obtained from RNA-seq analysis. Overall, we identified new functions of CmERF4 in negatively regulating chrysanthemum waterlogging tolerance by modulating energy metabolism and ROS pathway genes.
Cloud–cloud collision in S235: triggered the formation of high-mass stars and young star clusters
Authors: Chen En , Gao Yu , Zhang Shiyu , Chen Xuepeng , Fang Min , He Qianru , Jiang Xue-Jian , Sun Yan , Wang Xiao-Long , Ma Hongjun 
Published: November 2023

ABSTRACT
We present the analysis of cloud–cloud collision (CCC) process in the Galactic molecular complex S235. Our new CO observations performed with the PMO-13.7 m telescope reveal two molecular clouds, namely the S235-Main and the S235-ABC, with ∼4 km s−1 velocity separation. The bridge feature, the possible colliding interface and the complementary distribution of the two clouds are significant observational signatures of cloud–cloud collision in S235. The most direct evidence of cloud–cloud collision process in S235 is that the S235-Main (in a distance of 1547$^{+44}_{-43}$ pc) and S235-ABC (1567$^{+33}_{-39}$ pc) meet at almost the same position (within 1σ error range) at a supersonic relative speed. We identified ten 13CO clumps from PMO-13.7 m observations, 22 dust cores from the archival SCUBA-2 data, and 550 YSOs from NIR–MIR data. 63 per cent of total YSOs are clustering in seven MST groups (M1−M7). The tight association between the YSO groups (M1 $\&$ M7) and the bridge feature suggests that the CCC process triggers star formation there. The collisional impact subregion (the South) shows 3 ∼ 5 times higher CFE and SFE (average value of 12.3 and 10.6 per cent, respectively) than the non-collisional impact subregion (2.4 and 2.6 per cent, respectively), suggesting that the CCC process may have enhanced the CFE and SFE of the clouds compared to those without collision influence.
Unsupervised machine learning models reveal predictive clinical markers of glioblastoma patient survival using white blood cell counts prior to initiating chemoradiation
Authors: Wang Wesley , Kumm Zeynep Temerit , Ho Cindy , Zanesco-Fontes Ideli , Texiera Gustavo , Reis Rui Manuel , Martinetto Horacio , Khan Javaria , McCandless Martin G , Baker Katherine E , Anderson Mark D , Chohan Muhammad Omar , Beyer Sasha , Elder J Brad , Giglio Pierre , Otero José Javier 
Published: November 2023

Abstract

Background
Glioblastoma is a malignant brain tumor requiring careful clinical monitoring even after primary management. Personalized medicine has suggested the use of various molecular biomarkers as predictors of patient prognosis or factors utilized for clinical decision-making. However, the accessibility of such molecular testing poses a constraint for various institutes requiring identification of low-cost predictive biomarkers to ensure equitable care.

Methods
We collected retrospective data from patients seen at Ohio State University, University of Mississippi, Barretos Cancer Hospital (Brazil), and FLENI (Argentina) who were managed for glioblastoma—amounting to 581 patient records documented using REDCap. Patients were evaluated using an unsupervised machine learning approach comprised of dimensionality reduction and eigenvector analysis to visualize the inter-relationship of collected clinical features.

Results
We discovered that the serum white blood cell (WBC) count of a patient during baseline planning for treatment was predictive of overall survival with an over 6-month median survival difference between the upper and lower quartiles of WBC count. By utilizing an objective PD-L1 immunohistochemistry quantification algorithm, we were further able to identify an increase in PD-L1 expression in glioblastoma patients with high serum WBC counts.

Conclusions
These findings suggest that in a subset of glioblastoma patients the incorporation of WBC count and PD-L1 expression in the brain tumor biopsy as simple biomarkers predicting glioblastoma patient survival. Moreover, machine learning models allow the distillation of complex clinical data sets to uncover novel and meaningful clinical relationships.
A comparative study of microsurgery and gamma knife radiosurgery in vestibular schwannoma evaluating tumor control and functional outcome
Authors: Tatagiba Marcos , Wang Sophie S , Rizk Ahmed , Ebner Florian , van Eck Albertus T C J , Naros Georgios , Horstmann Gerhard 
Published: November 2023

Abstract

Background
Both stereotactic radiosurgery (SRS) and microsurgical resection (SURGERY) are available as treatment options for sporadic vestibular schwannoma (VS). There are very few direct comparative studies comparing both treatment modalities in large cohorts allowing detailed subgroup analysis. This present study aimed to compare the nuances in the treatment of VS by SURGERY and SRS in 2 highly specialized neurosurgical centers.

Methods
This is a retrospective bicentric cohort study. Data from patients treated between 2005 and 2011 were collected retrospectively. Recurrence-free survival (RFS) was assessed radiographically by contrast-enhanced magnetic resonance imaging.

Results
The study population included N = 901 patients with a mean follow-up of 7 years. Overall, the incidence of recurrence was 7% after SURGERY, and 11% after SRS with superior tumor control in SURGERY in the Kaplan–Meier-analysis (P = 0.031). In small tumors (Koos I and II), tumor control was equivalent in both treatment arms. In large VS (Koos III and IV), however, RFS was superior in SURGERY. The extent of resection correlated with RFS (P &lt; .001). Facial and hearing deterioration was similar in both treatment arms in small VS, but more pronounced in SURGERY of large VS. Tinnitus, vertigo, imbalance, and trigeminal symptoms were more often improved by SURGERY than SRS.

Conclusions
SRS can achieve similar tumor control compared to SURGERY in smaller VS (Koos I and II)—with similar postinterventional morbidities. In large VS (Koos III and IV), long-term tumor control of SRS is inferior to SURGERY. Based on these results, we suggest that if combination therapy is chosen, the residual tumor should not exceed the size of Koos II.
Effects of Pictorial Health Warning Labels on Intention to Quit Waterpipe in Lebanon: A Mediation Analysis
Authors: Jebai Rime , Asfar Taghrid , Cano Miguel Ángel , Nakkash Rima , Schmidt Michael , Wu Wensong , Bursac Zoran , Maziak Wasim 
Published: November 2023

Abstract

Introduction
Pictorial health warning labels (HWLs) on waterpipe (WP) tobacco packages represent a better strategy for communicating the health risks associated with WP use and promoting quit intention than text-only. However, the mechanism by which these warnings lead to higher intentions to quit remains unknown.

Aims and Methods
This study explores how pictorial warnings versus text-only induces higher quit intention among a sample of young adult WP smokers in Lebanon. An online randomized cross-over experimental study was conducted in August 2021 among 276 young adult WP smokers who were exposed to two conditions: pictorial HWLs and text-only warnings on WP tobacco packages in random order. After each image, participants completed post-exposure assessments of health communication outcomes (eg, attention and negative affect). Using serial and parallel mediation analysis, we examined the role of attention, negative affect, cognitive elaboration, and perceived harm in mediating the relationship between exposure to HWLs and intention to quit.

Results
Using serial mediation, exposure to pictorial warnings versus text-only was found to affect intention to quit through the following pathways: attention and negative affect, which accounted for 17.28% of the total effect, and through negative affect and cognitive elaboration, which accounted for 21.53% of the total effect. Results of parallel mediation showed that the indirect effect pathways via negative affect (β = 0.063; [95% bootstrap CI = 0.004 to 0.149]) and cognitive elaboration (0.047; [0.001 to 0.114]) were statistically significant.

Conclusions
The findings call on designing and implementing attention-grabbing, emotionally evocative, and cognitively persuasive pictorial warnings in Lebanon and other countries to curb WP tobacco smoking.

Implications
This is among the first experimental studies looking at multiple mediators underlying the effect of exposure to pictorial HWLs on intention to quit in a sample of current WP smokers in Lebanon. The findings showed that using attention-grabbing, emotionally evocative, and persuasive pictures with a clear textual warning is crucial to maximizing the impact of implementing WP-HWLs regulations in Lebanon and other countries to curb WP tobacco smoking.
Experimental analysis of development, lipid accumulation and gene expression in a high-latitude marine copepod
Authors: Roncalli Vittoria , Block Lauren N , Niestroy Jeanette L , Cieslak Matthew C , Castelfranco Ann M , Hartline Daniel K , Lenz Petra H 
Published: November 2023

Abstract
The high-latitude copepod Neocalanus flemingeri exploits the spring phytoplankton bloom to accumulate lipids for survival during food-limited periods and to fuel reproduction. At some point during development, lipid-accumulation ends and pre-adults molt into adults, descend to depth and enter a state of dormancy termed "diapause." How and when they determine to make this transition is still unresolved. According to one hypothesis, the trigger is their attaining a threshold amount of "lipid fullness." Alternatively, they might follow a genetic program, entering diapause within a narrow developmental window. To better understand the decision, a 5-week laboratory experiment was conducted to assess the effect of food quantity and type on lipid accumulation, biomass and gene expression in N. flemingeri copepodite stage CV. In fed individuals, the initial rate of lipid accumulation slowed by week 5, as a portion of CVs began to molt into adults. While changes in gene expression common to all fed individuals between weeks 1 and 3 were consistent with a developmental program, the duration of the CV stage was variable. Unfed individuals maintained lipid stores initially, suggesting physiological acclimatization to conserve energy. A comparison with gene expression profiles of field-collected individuals suggests similar responses to resources in the environment.
Trade-offs between cold protection and air pollution–induced mortality of China's heating policy
Authors: Zhang Haofan , He Pan , Liu Linxin , Dai Hui , Zhao Bin , Zeng Yi , Bi Jun , Liu Miaomiao , Ji John S 
Published: November 2023

AbstractThe winter heating policy in northern China was designed to safeguard households from the harsh subfreezing temperatures. However, it has inadvertently resulted in seasonal spikes in air pollution levels because of the reliance on coal as an energy source. While the loss of life years attributable to mortality from air pollution caused by winter heating has been estimated, the beneficial effect of protection from cold temperatures has not been assessed, primarily due to a lack of individual-level data linking these variables. Our study aims to address this research gap. We provide individual-level empirical evidence that quantifies the impact of protection from cold temperatures and air pollution on mortality, studying 5,334 older adults living around the Huai River during the period between 2000 and 2018. Our adjusted Cox-proportional hazard models show that winter heating was associated with a 22% lower mortality rate (95% CI: 16–28%). Individuals residing in areas without access to winter heating are subjected to heightened mortality risks during periods of cold temperatures. The protective effect is offset by a 27.8% rise attributed to elevated PM2.5 levels. Our results imply that the equilibrium between the effects of these two factors is achieved when PM2.5 concentration exceeds 24.3 µg/m3 (95% CI: 18.4–30.2). Our research suggests that while the existing winter heating policy significantly mitigates winter mortality by lessening the detrimental effects of cold temperatures, future air pollution reduction could provide further health benefits.
ALCON EYE CARE UK LTD v AMO DEVELOPMENT, LLC
Authors:   Patents Court
Published: November 2023

Abstract
H1 Patents—European patents—Revocation—Ophthalmic surgery—Cataract surgery—Validity—Characteristics and experience of the skilled person—Interaction between skilled team members—Common general knowledge—Negative or intermediate common general knowledge—Obviousness—Hindsight—Insufficiency—Evidence—Expert witnesses—Case management
Specificity in the processing of a subject’s own name
Authors: Bao Han , Xie Musi , Huang Ying , Liu Yutong , Lan Chuyi , Lin Zhiwei , Wang Yuzhi , Qin Pengmin 
Published: November 2023

Abstract
Subject’s own name (SON) is widely used in both daily life and the clinic. Event-related potential (ERP)-based studies have previously detected several ERP components related to SON processing; however, as most of these studies used SON as a deviant stimulus, it was not possible to determine whether these components were SON-specific. To identify SON-specific ERP components, we adopted a passive listening task with EEG data recording involving 25 subjects. The auditory stimuli were a SON, a friend’s name (FN), an unfamiliar name (UN) selected from other subjects’ names and seven different unfamiliar names (DUNs). The experimental settings included Equal-probabilistic, Frequent-SON, Frequent-FN and Frequent-UN conditions. The results showed that SON consistently evoked a frontocentral SON-related negativity (SRN) within 210–350 ms under all conditions, which was not detected with the other names. Meanwhile, a late positive potential evoked by SON was found to be affected by stimulus probability, showing no significant difference between the SON and the other names in the Frequent-SON condition, or between the SON and a FN in the Frequent-UN condition. Taken together, our findings indicated that the SRN was a SON-specific ERP component, suggesting that distinct neural mechanism underly the processing of a SON.
Bridging conflicting frames in policies for digital transformation
Authors: Coco Nunzia , Colapinto Cinzia , Finotto Vladi 
Published: November 2023

Abstract
Despite several policies aimed at igniting digital transformation, many regions lag behind. We investigate this discrepancy by postulating that the political consensus on accelerating Industry 4.0 adoption among micro-, small- and mid-sized enterprises (MSMEs) needs to consider the heterogeneity of entrepreneurial and social fabrics. Best practices in policy-making exist, but their deployment requires adaptations. The present paper delves into such adaptations and on the mechanics behind how a ‘model’ is modified locally. We do so by illustrating the results of action research on a regional policy initiative. Our findings point to one important set of factors and mechanisms conducive to the adaptation of a policy model to the peculiarities of a locale via the alignment of different cognitive frames: co-design processes. We elaborate on the role of different actors, the relevance of ‘hybrid’ actors, and the nature of universities as platforms
Hyperbaric oxygen therapy as a treatment for erectile dysfunction: a meta-analysis
Authors: Saffati Gal , Naeem Taher , Guhan Maya , Abello Alejandro , Hinojosa-Gonzalez David Eugenio , Kaaki Basil , Stocks Blair T , Lipshultz Larry I , Khera Mohit 
Published: November 2023

Abstract

Introduction
Hyperbaric oxygen therapy (HBOT) is a medical treatment in which the patient is exposed to 100% oxygen at a higher than atmospheric pressure. Over the past few decades, HBOT has been used to treat a variety of medical conditions. In recent times, there has been a rising curiosity regarding the potential therapeutic benefits of HBOT in the treatment of erectile dysfunction (ED).

Aims
The study sought to review and meta-analyze available data regarding the use of HBOT for ED, including its potential mechanisms of action and effectiveness.

Methods
We included only articles that evaluated the impact of HBOT on ED symptoms using the International Index of Erectile Function score. Prospective nonrandomized studies or randomized controlled clinical trials were included. Data extraction was performed in duplicate. Data analysis was conducted using Review Manager 5.41, and the presence of heterogeneity between studies was evaluated. The results were presented as the mean difference (MD) with 95% confidence interval (CI).

Results
A total of 5 studies that reported outcomes using the International Index of Erectile Function scores were included in this analysis. In patients with post–robotic-assisted laparoscopic prostatectomy–induced ED, the analysis showed a significant MD of −4.13 (95% CI, −6.08 to −2.18; P &lt; .0001) in favor of the control group. Conversely, patients who received HBOT for reasons other than ED exhibited an MD of 4.58 (95% CI, 2.63 to 6.52; P &lt; .00001). In the group that received HBOT for pure vasculogenic ED, the MD was 10.50 (95% CI, 9.92 to 11.08) in favor of HBOT. A meta-analysis of these data revealed a nonsignificant difference in erectile function scores, with an MD of 3.86 (95% CI, −2.13 to 9.86; P = .21).

Conclusion
The use of HBOT in the treatment of ED appears to be a promising approach. While further research is needed to establish the efficacy and long-term effects of this treatment, preliminary studies have shown encouraging results in terms of improving erectile function in men with vasculogenic ED.