bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomised clinical trial study assessing the efficacy of 5% losartan potassium loaded in ethosomal gel to treat human keloids: a trial protocol
Authors: Anggraini Yuni Eka , Trisnowati Niken , Martien Ronny , Danarti Retno 
Published: January 2024

AbstractBackgroundKeloid is a skin disorder that results from excessive fibrous tissue growth in the area of the initial trauma. Treating keloids can be challenging since the success of various treatments varies from one study to another. Triamcinolone acetonide injection, a standard treatment, can cause undesirable side effects. Meanwhile, the effectiveness of existing topical therapies for keloids is not always reliable. The pro-inflammatory, pro-proliferative, and pro-fibrotic effects of angiotensin II in human skin contribute to keloid formation. Losartan potassium, an angiotensin II blocker, has the potential to act as an anti-keloid agent. Due to the thicker skin structure of a keloid and ease of application, ethosome gel is chosen as a safe and comfortable carrier for losartan potassium, making it a good choice for treating keloids.MethodsIn this randomised clinical trial, 46 adults with keloids were divided into two treatment groups. One group of 23 participants received 5% losartan potassium loaded in ethosomal gel, while the other group of 23 participants received intralesional injections of 10% triamcinolone acetonide. Over 12 weeks, changes in POSAS 3.0 scores, degree of erythema and pigmentation, surface area, thickness, and pliability of the keloids will be measured at four different times: baseline, 4 weeks, 8 weeks, and 12 weeks. Statistical analysis will be conducted using SPSS software version 24, with a significance level ofp< 0.05.DiscussionLosartan potassium is believed to be beneficial for keloid management because it inhibits the angiotensin II receptor, which plays a role in inflammation, proliferation, and fibrosis. This study examines the efficacy of 5% losartan potassium loaded in ethosomal gel for human keloids.Trial registrationClinicaltrial.gov identifierNCT05893108. Registered on 7 June 2023.
A prevention program for binge drinking among students based on mindfulness and implementation intention (ALCOMEDIIT): a randomized controlled trial
Authors: Mange Jessica , Mauny Nicolas , Montcharmont Charlotte , Legrand Eve , Lemercier-Dugarin Maud , Mortier Arnaud , Duvivier Martin , Leveneur Johnny , Lacherez Cédric , Cabé Nicolas , Le Berre Anne-Pascale 
Published: January 2024

Abstract
Background
The emergence of new problematic alcohol consumption practices among young people requires new dynamics in prevention strategies. In this context, the ADUC project (Alcohol and Drugs at the University of Caen) aims to develop a better understanding of alcohol consumption, and in particular the practice of binge drinking (BD) in students, in order to develop relevant and adapted prevention tools. The ALCOMEDIIT study (Rin Normandie and IRESP funding; Agreement 20II31-00 - ADUC part 3) is a randomized controlled trial that focuses on the specific determinant of impulsivity. The main objective of this experiment is to assess a program for the prevention of BD practices based on motivational interviewing (MI) associated with implementation intention (II) and mindfulness meditation (MBM) in a student environment.

Methods
This study will include 170 healthy subjects who will be students at the university, alcohol users, with a BD score > 1 in the month preceding the inclusion but not presenting any specific disorder. The trial will be proposed by e-mail and students who meet the inclusion criteria will join either a control group which will benefit from a MI or an experimental group which will additionally benefit from an initiation to MBM with II (initial visit T0). In order to measure the effectiveness of the prevention program in terms of BD decrease, a follow-up at 1 month (T1) as well as a follow-up at 6 months (T6; exploratory) will be proposed to all participants. The total duration of this research protocol is 21 months.

Discussion
The purpose of this study is to evaluate the interest of associating mindfulness meditation practices and implementation of self-regulation strategies to optimize their use, with a motivational interview in an innovative prevention program aiming at reducing alcohol use and BD practice in the student population.

Trial registration
ClinicalTrials.gov Identifier: NCT05565989, September 30, 2022.
https://clinicaltrials.gov/study/NCT05565989
Protocol version 2.0 (September 2022)
No. ID-RCB : 2022-A00983-40
Endovascular aortic repair with sac embolization for the prevention of type II endoleaks (the EVAR-SE study): study protocol for a randomized controlled multicentre study in Germany
Authors: Knappich Christoph , Kirchhoff Felix , Fritsche Marie-Kristin , Egert-Schwender Silvia , Wendorff Heiko , Kallmayer Michael , Haller Bernhard , Hyhlik-Duerr Alexander , Reeps Christian , Eckstein Hans-Henning , Trenner Matthias 
Published: January 2024

Abstract
Background
Beyond a certain threshold diameter, abdominal aortic aneurysms (AAA) are to be treated by open surgical or endovascular aortic aneurysm repair (EVAR). In a quarter of patients who undergo EVAR, inversion of blood flow in the inferior mesenteric artery or lumbar arteries may lead to type II endoleak (T2EL), which is associated with complications (e.g. AAA growth, secondary type I endoleak, rupture). As secondary interventions to treat T2EL often fail and may be highly invasive, prevention of T2EL is desirable. The present study aims to assess the efficacy of sac embolization (SE) with metal coils during EVAR to prevent T2EL in patients at high risk.

Methods
Over a 24-month recruitment period, a total of 100 patients undergoing EVAR in four vascular centres (i.e. Klinikum rechts der Isar of the Technical University of Munich, University Hospital Augsburg, University Hospital Dresden, St. Joseph’s Hospital Wiesbaden) are to be included in the present study. Patients at high risk for T2EL (i.e. ≥ 5 efferent vessels covered by endograft or aneurysmal thrombus volume <40%) are randomized to one group receiving standard EVAR and another group receiving EVAR with SE. Follow-up assessments postoperatively, after 30 days, and 6 months involve contrast-enhanced ultrasound scans (CEUS) and after 12 months an additional computed tomography angiography (CTA) scan. The presence of T2EL detected by CEUS or CTA after 12 months is the primary endpoint. Secondary endpoints comprise quality of life (quantified by the SF-36 questionnaire), reintervention rate, occurrence of type I/III endoleak, aortic rupture, death, alteration of aneurysm volume, or diameter. Standardized evaluation of CTA scans happens through a core lab. The study will be terminated after the final follow-up visit of the ultimate patient.

Discussion
Although preexisting studies repeatedly indicated a beneficial effect of SE on T2EL rates after EVAR, patient relevant outcomes have not been assessed until now. The present study is the first randomized controlled multicentre study to assess the impact of SE on quality of life. Further unique features include employment of easily assessable high-risk criteria, a contemporary follow-up protocol, and approval to use any commercially available coil material. Overcoming limitations of previous studies might help SE to be implemented in daily practice and to enhance patient safety.

Trial registration
ClinicalTrials.gov NCT05665101. Registered on 23 December 2022.
Nailfold videocapillaroscopy in antineutrophil cytoplasmic antibody–associated vasculitis
Authors: Sullivan Megan M. , Abril Andy , Aslam Nabeel , Ball Colleen T. , Berianu Florentina 
Published: January 2024

Abstract
Objective
Antineutrophil cytoplasmic antibody (ANCA)–associated vasculitis (AAV) is a group of illnesses that cause inflammation and alterations to small vessels in the body. Some of the most common and detrimental manifestations, including alveolar hemorrhage and glomerulonephritis, are caused by this capillary inflammation. We sought to clarify whether patients with AAV would have abnormal nailfold capillaries when evaluated with nailfold videocapillaroscopy.

Methods
Patients with a current diagnosis of AAV and a control group were identified for enrollment. Nailfold videocapillaroscopy images were used for a semiquantitative analysis on capillary density, morphology, dilation, and microhemorrhage after review by 2 rheumatologists. Disease characteristics, occurrence of recent disease flare, and presence of ANCA were recorded.

Results
Thirty-three patients with a diagnosis of AAV and 21 controls were recruited. The AAV group had a median age of 59 and 17 (52%) were women. Granulomatosis with polyangiitis was the most common diagnosis (19 [58%]), followed by eosinophilic granulomatosis with polyangiitis (7 [21%]) and microscopic polyangiitis (7 [21%]). Twenty-seven patients (82%) had positive ANCA tests. After assessment of capillary density, dilation, morphology, microhemorrhages, and disorganization, there were no statistically significant differences between the 2 groups.

Conclusion
There was no evidence of differences in nailfold capillaroscopy abnormalities between those diagnosed with AAV and the control group. While this cohort was relatively small, we did not find a high enough prevalence or specific phenotype of capillary abnormalities that could aid in diagnosis or prognostication of these diseases in the clinical setting.
Fatigue effects on the knee flexors neuromuscular parameters during repeated sprinting
Authors: Pimenta Ricardo , Lopes Tomás , Veloso A. P. 
Published: January 2024

Abstract
Background
To identify at which point fatigue on neuromuscular parameters occurs in the knee flexors during a repeated sprint protocol.

Methods
Physical active males without previous hamstring strain injury were recruited. Neuromuscular parameters such as peak torque (PT) and rate of torque development (RTD) were assessed after every two sprints in a 5 × (2 x 30 m) repeated sprint protocol.

Results
Twenty physical active males participated in the study. A significant effect of sprint number was found (p < 0.001; η2p = 0.643) with a decreased sprint speed by 6.9% from fastest to slowest sprint. No significant differences were observed in the time between finishing the sprint and performing the first MVIC (46.3 ± 4.7s; p = 0.423), nor in the time between finishing a set and starting the next set (121.2 ± 7.6s; p = 0.503). Regarding neuromuscular parameters, the only significant difference found was in PT between before and after two sprints (117.95 ± 5.61 N⋅m vs. 110.64 ± 5.71 N⋅m; p = 0.048, d = 0.289) and on RTD 0-50ms before and after ten sprints (465.78 ± 223.76 N⋅m/s vs. 382.30 ± 189.56 N⋅m/s; p = 0.008; η2p = 0.149).

Conclusions
A recovery time of 46s between sprints and testing neuromuscular parameters (due to experimental design) seems sufficient to restore the neuromuscular system. Therefore, it can be suggested that time recovery is the principal factor in detecting fatigue on neuromuscular parameters.
Accuracy validation of a wearable IMU-based gait analysis in healthy female
Authors: He Yi , Chen Yuxia , Tang Li , Chen Jing , Tang Jing , Yang Xiaoxuan , Su Songchuan , Zhao Chen , Xiao Nong 
Published: January 2024

Abstract
Objective
The aim of this study was to assess the accuracy and test-retest reliability of a wearable inertial measurement unit (IMU) system for gait analysis in healthy female compared to a gold-standard optoelectronic motion capture (OMC) system.

Methods
In our study, we collected data from 5 healthy young females. Participants were attached with markers from both the OMC system and the IMU system simultaneously. Data was collected when participants walked on a 7 m walking path. Each participant performed 50 repetitions of walking on the path. To ensure the collection of complete gait cycle data, a gait cycle was considered valid only if the participant passed through the center of the walking path at the same time that the OMC system detected a valid marker signal. As a result, 5 gait cycles that met the standards of the OMC system were included in the final analysis. The stride length, cadence, velocity, stance phase and swing phase of the spatio-temporal parameters were included in the analysis. A generalized linear mixture model was used to assess the repeatability of the two systems. The Wilcoxon rank-sum test for continuous variables was used to compare the mean differences between the two systems. For evaluating the reliability of the IMU system, we calculated the Intra-class Correlation Coefficient (ICC). Additionally, Bland-Altman plots were used to compare the levels of agreement between the two systems.

Results
The measurements of Spatio-temporal parameters, including the stance phase (P = 0.78, 0.13, L-R), swing phase (P = 0.78, 0.13, L-R), velocity (P = 0.14, 0.13, L-R), cadence (P = 0.53, 0.22, L-R), stride length (P = 0.05, 0.19, L-R), by the IMU system and OMC system were similar. Which suggested that IMU and OMC systems could be used interchangeably for gait measurements. The intra-rater reliability showed an excellent correlation for the stance phase, swing phase, velocity and cadence (Intraclass Correlation Coefficient, ICC > 0.9) for both systems. However, the correlation of stride length was poor (ICC = 0.36, P = 0.34, L) to medium (ICC = 0.56, P = 0.22, R). Additionally, the measurements of IMU systems were repeatable.

Conclusions
The results of IMU system and OMC system shown good repeatability. Wearable IMU system could analyze gait data accurately. In particular, the measurement of stance phase, swing phase, velocity and cadence showed excellent reliability. IMU system provided an alternative measurement to OMC for gait analysis. However, the measurement of stride length by IMU needs further consideration.
Variability of unilateral and bilateral isometric muscle strength of lower extremities extensors in young females and males
Authors: Kabacinski Jaroslaw , Murawa Michal , Wojtkowiak Tadeusz , Mackala Krzysztof , Dworak Lechoslaw B. 
Published: January 2024

AbstractBackgroundThe muscle strength of the lower extremity extensors can be evaluated in the closed kinetic chain (CKC) during unilateral or bilateral conditions. Factors such as the mass and length of the muscle, joint angle, type of contraction, and gender influence the magnitude of the muscle strength. The aim of this study was to compare the isometric strength of lower extremity extensors between the different knee extension angles (KEs) as well as between bilateral and unilateral conditions.MethodsNineteen female students (age: 20.2 ± 0.6 years) and nineteen male students (age: 20.3 ± 0.7 years) participated in the study. The muscle strength was evaluated in CKC using the strain gauge dynamometer. The analysis included values of the maximum muscle strength normalized to body mass (MS/BM) for the six KEs of 80°, 70°, 60°, 50°, 40° and 30°.ResultsA significant main effect in the MS/BM values for the angle factor (p < 0.001) and condition factor (p < 0.001) was found. Moreover, there was a non-significant interaction effect between the angle factor and gender factor (p = 0.476) as well as between the condition factor and gender factor (p = 0.770). Comparisons showed significant differences in the MS/BM values between the six KEs (p < 0.001). Furthermore, significantly lower MS/BM values for bilateral conditions than unilateral conditions at the 30° KE were observed (p < 0.001).ConclusionThe decrease in KE by 10° significantly increased the muscle strength of the lower extremity extensors. Gender did not affect the change in MS/BM values with the change in KE and conditions. Findings also revealed significant bilateral deficit, i.e., significantly a lower summed muscle strength during bilateral conditions than unilateral conditions. The study emphasized the importance of selecting the 30° KE as the optimal angle to assess the maximum strength developed in CKC.
Metronomic cyclophosphamide and metformin inhibited tumor growth and repopulated tumor-infiltrating lymphocytes in an experimental carcinoma model
Authors: Zaki Heba Mohamed , Ali Khadiga Mohamed , Abd Allah Mona Younis Youssef , Abouelnaga Amoura Mohamed , Abdraboh Mohamed Elsaid , Hussein Osama 
Published: January 2024

AbstractMetformin is a widely used antidiabetic biguanide. Retrospective data demonstrated the association of metformin use with survival benefit in multiple tumor types. Interest in repurposing metformin to treat cancer has not been translated into encouraging clinical benefit. In animal models, metformin activated cytotoxic T cells and exerted an immune-mediated anticancer effect. The current research was conducted to investigate the possible therapeutic benefit of metformin in combination with metronomic cyclophosphamide in an experimental cancer model. Ehrlich ascites carcinoma was injected into the subcutaneous tissue to induce solid tumors in syngeneic mice. Exponential solid tumor growth ensued and was effectively arrested with the administration of a cytotoxic dose of parenteral cyclophosphamide. Alternatively, oral metformin and continuous, low-dose cyclophosphamide significantly inhibited tumor growth relative to untreated mice. The drug combination was well tolerated. Histopathological examination of the tumor showed an increased number of tumor-infiltrating lymphocytes and enhanced expression of granzyme B by this drug combination. The current data suggests a potential role of metformin and metronomic chemotherapy that warrants further investigation.
Trust in adolescence and depression and anxiety symptoms in young adulthood: findings from a Swedish cohort
Authors: Låftman Sara Brolin , Raninen Jonas , Östberg Viveca 
Published: January 2024

Abstract
Objective
Trust and health are both fundamental elements of a socially sustainable society. While much research has shown that trust is associated with better mental health outcomes in adults, studies of young people are relatively scarce, despite the fact that mental health problems are common in young ages. In particular, there are few longitudinal studies that cover different dimensions of trust. Building on a previous study on trust and psychosomatic complaints in adolescents, the aim was to examine the links between generalised and institutional trust in adolescence and depression and anxiety symptoms in young adulthood. Data was obtained from a Swedish cohort study with self-reported information on generalised and institutional trust at ages 15–16 and 17–18 and depression and anxiety symptoms at age 20–21 (n = 2,668). Covariates included sociodemographic characteristics and indicators of prior mental health status.

Results
Binary logistic and linear regressions showed that higher levels of generalised trust at ages 15–16 and 17–18 were inversely associated with depression and anxiety symptoms at age 20–21. Institutional trust was however not linked with subsequent depression and anxiety symptoms when adjusting for generalised trust and covariates. The findings indicate that generalised trust is a social determinant for mental health in young people.
Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney
Authors: Jiang Yongwei , Deng GuoXiong , Liu Chengyin , Tang Han , Zheng Jing , Kong Xiaomu , Zhao Meimei , Liu Yi , Gao Peng , Li Tianbao , Zhao Hailing , Cao Yongtong , Li Ping , Ma Liang 
Published: January 2024

Abstract
Background
The objective of this study was to examine and analyze differential methylation profiles in order to investigate the influence of hyper-methioninemia (HM) on the development of diabetic nephropathy (DN). Male Wistar rats, aged eight weeks and weighing 250–300 g, were randomly assigned into four groups: a control group (Healthy, n = 8), streptozocin-induced rats (STZ group, n = 8), HM + STZ group (n = 8), and the Tangshen Formula (TSF) treatment group (TSF group, n = 8). Blood glucose levels and other metabolic indicators were monitored before treatment and at four-week intervals until 12 weeks. Total DNA was extracted from the aforementioned groups, and DNA methylation landscapes were analyzed via reduced representative bisulfite sequencing.

Results
Both the STZ group and HM + STZ group exhibited increased blood glucose levels and urinary albumin/creatinine ratios in comparison with the control group. Notably, the HM + STZ group exhibited a markedly elevated urinary albumin/creatinine ratio (411.90 ± 88.86 mg/g) compared to the STZ group (238.41 ± 62.52 mg/g). TSF-treated rats demonstrated substantial reductions in both blood glucose levels and urinary albumin/creatinine ratios in comparison with the HM + STZ group. In-depth analysis of DNA methylation profiles revealed 797 genes with potential therapeutic effects related to TSF, among which approximately 2.3% had been previously reported as homologous genes.

Conclusion
While HM exacerbates DN through altered methylation patterns at specific CpG sites, TSF holds promise as a viable treatment for DN by restoring abnormal methylation levels. The identification of specific genes provides valuable insights into the underlying mechanisms of DN pathogenesis and offers potential therapeutic targets for further investigation.
Interest in genetic susceptibility testing and disclosure of AD dementia risk in cognitively normal adults: a survey study
Authors: Waterink Lisa , Masselink Larissa A. , van der Lee Sven J. , Visser Leonie N. C. , Cleutjens Solange , van der Schaar Jetske , van Harten Argonde C. , Scheltens Philip , Sikkes Sietske A. M. , van der Flier Wiesje M. , Zwan Marissa D. 
Published: January 2024

Abstract
Background
Apolipoprotein-E (APOE) genetic testing for Alzheimer’s disease is becoming more important as clinical trials are increasingly targeting individuals carrying APOE-ε4 alleles. Little is known about the interest in finding out one’s genetic risk for Alzheimer’s disease in the general population. Our objective was to examine this in a sample of cognitively normal (CN) adults within a population-based online research registry with the goal to implement APOE-ε4 status for trial recruitment.

Methods
An online survey was completed by 442 CN participants between the age of 49 and 75 years (56% female) from the Dutch Brain Research Registry. The survey assessed interest in participation in research into, and disclosure of, genetic risk for dementia. The survey assessed interest in participation in research into, and disclosure of, genetic risk for dementia and knowing their genetic risk in different hypothetical risk scenarios (10%, 30%, and 50% genetic risk for dementia at age 85, corresponding to APOEε2/ε2 or ε2/ε3, APOEε3/ε4 or ε2ε4, and APOE-ε4/ε4 genotypes). Cochran’s Q and post hoc McNemar tests were used to analyse differences in frequencies across scenarios.

Results
Most participants were interested in participating in research into and disclosure of their genetic risk (81%). The most reported reason was to contribute to scientific research (94%). Interest was higher in males, whilst lower-educated participants were more often undecided. When provided with different risk scenarios, interest in knowing their risk was somewhat higher in the scenarios with higher risk, i.e. in the 50% (79%) compared to the 10% scenario (73%;χ2(2) = 7.98; p = .005). Most individuals expected they would share their genetic risk with close relatives (77–89%), would participate in medication trials (79–88%), and would make long-term arrangements, e.g. retirement, health care, will (69–82%), with larger proportions for scenarios with higher hypothetical genetic risk.

Conclusions
Our findings indicate that the vast majority of CN adults participating in a research registry expresses interest in AD genetic risk research and disclosure. Interest in genetic risk disclosure is higher in scenarios corresponding to the APOE-ε4 genotype. This suggests APOE-ε4 screening within an online research registry is potentially a well-received method to accelerate inclusion for trials.