bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the physical-mechanical behavior of the polymer composite reinforced with silicon flotation residue
Authors: Banna Wassim El , Xavier Wendel de Jesus da Cruz , Costa Deibson Silva da , Pinheiro Darllan do Rosário 
Published: October 2023

Abstract
The use of composite materials has seen a significant increase for economic, performance, and environmental reasons. Consequently, improperly discarded industrial waste has been employed as particles in composite materials. Thus, this study addresses the fabrication of polyester matrix composites reinforced with in natura silicon residue particles. In this regard, the manual hand lay-up method was used in the fabrication process, employing rectangular metal molds. Silicon residue was incorporated at mass fractions of 5%, 10%, and 15%. The manufactured composites were analyzed for mechanical properties (tensile and flexural) and physical properties (density, apparent density, water absorption, and apparent porosity). The results showed that the mechanical characteristics of the materials changed with varying mass fractions. X-ray Diffraction (XRD) analysis provided insights into certain material properties based on the identified materials. The studied composite achieved a flammability classification according to established standards. Furthermore, it exhibited tensile strength (37.28 ± 4.87 MPa) and flexural strength (114.17 ± 12.09 MPa).
Assessing Genetic Diversity in Critically Endangered Chieniodendron hainanense Populations within Fragmented Habitats in Hainan
Authors: Chen Yukai , Zhang Hai-Li , Zhang Li , Wu Tingtian , Liu Tingting , Zhou Qin , Nizamani Mir Muhammad 
Published: October 2023

Abstract

Habitat fragmentation engenders a reduction in the geographic distribution of species, thereby rendering diminutive populations susceptible to extinction due to environmental, demographic, and genetic factors. Chieniodendron hainanense (henceforth C. hainanense) exemplifies a wild plant with extremely small populations (WPESP) and faces endangerment, necessitating urgent national conservation efforts. Elucidating the genetic diversity of C. hainanense is crucial for uncovering underlying mechanisms and devising protective strategies. In the present study, 35 specimens from six distinct cohort groups were genotyped utilizing genotyping-by-sequencing (GBS) and single nucleotide polymorphism (SNP) methodologies. The results indicated that C. hainanense exhibits limited genetic diversity. Observed heterozygosity within C. hainanensepopulations spanned from 10.79% to 14.55%, with an average value of 13.15%. The six C. hainanense populations can be categorized into two distinct groups: (1) Diaoluoshan and Baishaling, and (2) Wuzhishan, Huishan, Bawangling, and Jianfengling. The degree of genetic differentiation among C. hainanensepopulations is relatively weak. The observed loss of diversity can be attributed to the effects of natural selection.
Macroscopic inhibition of DNA damage repair pathways by targeting TFAP2A with LEI110 eradicates hepatocellular carcinoma
Authors: Wang Chenchen , Zhao Zhenjun , Zhao Yudon , Xia Lei , Qiang Xia 
Published: October 2023

Abstract
Hepatocellular carcinoma (HCC) is one of the world’s leading causes of cancer-related death and DNA damage repair-related genes were closed related to the progression of HCC. Combining the copy number variation and the expression profile of DNA damage repair-related genes, we observed a unique cluster of “deletion-up” genes in HCC, which strongly correlated with the prognosis of liver cancer. Binding motif analysis and further validation with ChIP-qPCR/dual luciferase assay unveiled that the TFAP2A could modulate the transcription of critical DNA repair genes including TOP2A, NUDT1, POLD1, and PARP1, which eventually facilitated the sanitation of oxidized DNA lesions in cells. Structural analysis and the following validation identified LEI110 as a potent TFAP2A inhibitor. We demonstrated that LEI110 could stabilize TFAP2A and sensitize HCC cells towards different DNA damaging reagents in liver cancer cells. Together, we identified TFAP2A as a crucial transcription modulator for a unique cluster of “deletion-up” genes in HCC and identified LEI110 as a potential inhibitor of TFAP2A which sensitized HCC to DNA-damaging reagents. Our study provide insights into a concept of macroscopic inhibition of DNA damage repair-related genes in the cancer treatment.
Uni-pKa: An Accurate and Physically Consistent pKa Prediction through Protonation Ensemble Modeling
Authors: Zheng Hang , Luo Weiliang , Zhou Gengmo , Zhu Zhengdan , Yuan Yannan , Ke Guolin , Wei Zhewei , Gao Zhifeng 
Published: October 2023

Abstract
Predicting pKa values of small molecules has key applications in drug discovery and molecular simulation. However, current methods face challenges in rigorously interpreting experimental data and ensuring thermodynamic consistency between successive pKa values. To address these limitations, we present Uni-pKa, an accurate and reliable pKa prediction framework. Uni-pKa is based on comprehensive free energy modeling of possible molecules in protonation equilibrium. Within this framework, a structural enumerator recovers underlying structures in pKa datapoints, and a neural network serves as a free energy predictor, learning from data rigorously while inherently preserving thermodynamic consistency. Through a pretraining-finetuning strategy utilizing predicted and experimental pKa data, Uni-pKa achieves state-of-the-art accuracy among chemoinformatic methods. Uni-pKa provides a good example of combining chemical principles and machine learning to solve scientific problems.
Sowing Seeds of Purpose: How Green HRM Practices Elevate Employee Experience
Authors: Sidique Nazif , Pereira Leandro , Dias Alvaro 
Published: October 2023

Abstract
Green Human Resource Management (GHRM) merges environmental and HR practices, promoting green behavior across organizations. This quantitative study examines the effects of GHRM on employees' experiences and perceptions. Results indicate that a higher inclination to adopt GHRM correlates with an enhanced sense of purpose in work, leading to a positive work ambiance. This motivation also ties to greater job satisfaction, emphasizing the significance of aligning individual values with organizational objectives. Notably, organizations with robust GHRM leadership foster greater employee loyalty, underscoring GHRM's role in boosting commitment and cohesion. These insights shed light on GHRM's profound influence on employee welfare and organizational dynamics. Future research should investigate the long-term effects of GHRM on employee behavior.
Property Deterioration in Reactive Elastomeric Terpolymer Modified Binders During Storage at Elevated Temperatures
Authors: Sharma Alok , Pandey Akanksha , R.N GD Ransinchung , Ravindrananth Sham S. 
Published: October 2023

Abstract
Polymer-modified binders are often stored and transported at elevated temperatures, where their properties may deteriorate if mishandled. This study investigates the effect of storage temperature, storage time, base binder, and PPA concentration on the conventional and rheological properties of commercial-grade reactive elastomeric terpolymer-modified binders (RET-MBs). To mimic practical storage conditions, RET-MB was contained in sealed metal tubes and stored in a forced convection oven at temperatures of 120, 135, 150, and 165°C for up to 14 days. The results show that the deterioration in conventional and rheological properties of RET-MB was noticeable even at a low storage temperature of 135°C. At temperatures of 150 and 165°C, significant erosion in properties was observed within the first three days of storage. After 7 days of storage at 150°C, the softening point, Tu, and % elastic recovery values decrease by 4°C, 3.5°C, and 46%, respectively. One PG-grade drop can occur after 3–7 days of storage at 150 and 165°C. FTIR analysis of the solution-cast films of RET polymer reveals a strong interaction of the epoxy ring with PPA molecules to form phosphate ester linkage during storage, due to which the interaction between RET polymer and binder molecules reduces, resulting in property erosion during storage. The results show that RET-MBs should be stored and transported at temperatures < 135°C.
NK Receptors Replace CD28 As the Dominant Source of Signal 2 for Cognate Recognition of Cancer Cells by TAA-specific Effector CD8+ T Cells
Authors: Kalinski Pawel , Dong Bowen , Obermajer Natasa , Tsuji Takemasa , Matsuzaki Junko , Bonura Cindy , Withers Henry , Long Mark , Chavel Colin , Olejniczak Scott , Minderman Hans , Edwards Robert , Storkus Walter , Romero Pedro 
Published: October 2023

Abstract
CD28-driven “signal 2” is critical for naïve CD8+ T cell responses to dendritic cell (DC)-presented weak antigens, including non-mutated tumor-associated antigens (TAAs). However, it is unclear how DC-primed cytotoxic T lymphocytes (CTLs) respond to the same TAAs presented by cancer cells which lack CD28 ligands. Here, we show that NK receptors (NKRs) DNAM-1 and NKG2D replace CD28 during CTL re-activation by cancer cells presenting low levels of MHC I/TAA complexes, leading to enhanced proximal TCR signaling, immune synapse formation, CTL polyfunctionality, release of cytolytic granules and antigen-specific cancer cell killing. Double-transduction of T cells with recombinant TCR and NKR constructs or upregulation of NKR-ligand expression on cancer cells by chemotherapy enabled effective recognition and killing of poorly immunogenic tumor cells by CTLs. Operational synergy between TCR and NKRs in CTL recognition explains the ability of cancer-expressed self-antigens to serve as tumor rejection antigens, helping to develop more effective therapies.
Reference interval proposal for fasting insulin and HOMA-IR measurement based on access to an extensive big Brazilian laboratory database.
Authors: Schrank Yolanda , Fontes Rosita , Perozo Andrea Faria Dutra Fragoso , Araújo Paula Bruna , Pinheiro Maria Fernanda Miguens Castelar , Gomes Dalva Margareth Valente , Vieira Luisane Maria Falci 
Published: October 2023

Abstract
Objectives: Fasting insulin and the homeostatic model assessment of insulin resistance (HOMA-IR) index are relatively simple and reliable noninvasive markers of insulin resistance (IR). Given the relevance of correctly diagnosing IR, we emphasize the importance of establishing reliable reference intervals (RIs) for these markers.
This study aimed to determine the reference RIs of fasting insulin and HOMA-IR index in adults living in Rio de Janeiro – Brazil, and, secondarily, to verify if there was any difference in RIs between sexes.
Methods: Fasting serum insulin levels of 146497 (20 to 60 years old) subjects submitted to blood sampling from January to December of 2019 in Rio de Janeiro were obtained retrospectively through access to an extensive laboratory database. Insulin was determined by the electrochemiluminescence immunoassay (ECLIA) method, using Roche Diagnostics kits. After applying exclusion criteria 21684 subjects [18576 (86%) women] were included (average 36 ± 3.66 years). Reference interval was established using a validated mining algorithm named Laboratory Reference Interval (LabRI).
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Results: Overall, 95% RIs for fasting insulin levels were, respectively, 2.54-13.30, 2.43-11.89, and 2.52-13.14 μU/mL (15.3-80.12, 14.6-71.7, and 15.2-79.2 pmol/L) in women, men, and the total population. HOMA-IR index RIs were 0.39-2.86, 0.38-2.81, and 0.39-2.86 in women, men, and the total population. Although the difference in insulin levels and HOMA-IR index between men and women were statistically significant, it does not justify using sex-specific RIs.
Conclusion: We conclude that RIs of fasting insulin and HOMA-IR index found in the total population can be applied to both sexes. Thus, we suggest the following RIs for our population: fasting Insulin: 2.52-13.14 μU/mL (15.1-78.8 pmol/L) and HOMA-IR index: 0.39-2.86.
Spontaneous creation of muons and destruction of protons in ultra- dense hydrogen H(0)
Authors: Holmlid Leif 
Published: October 2023

Abstract
A patented laser-induced muon generator (Holmlid 2017) is here used to observe also the spontaneous formation of muons from ultra-dense hydrogen H(0). The muons are detected by their interaction with converters and scintillators both inside and outside the vacuum apparatus containing the muon generator. This interaction creates x-ray radiation which then gives beta e+e- pairs with a zero energy cutoff of 510 ± 10 keV. The e+e- energy distributions are matched by a statistical model. It is concluded that muons are formed by spontaneous annihilation nuclear processes in H(0) primarily creating mesons. The lifetime before the spontaneous proton destruction by annihilation is estimated from the results to be of the order of 1011 years, thus much shorter than theoretical proton lifetimes.
Effects of Metformin on Transcriptomic and Metabolomic Profiles in Breast Cancer Survivors Enrolled in the Randomized Placebo-Controlled MetBreCS Trial
Authors: Strømland Pouda Panahandeh , Bertelsen Bjørn-Erik , Viste Kristin , Chatziioannou Anastasia Chrysovalantou , Bellerba Federica , Robinot Nivonirina , Trolat Amarine , Flågeng Marianne Hauglid , Scalbert Augustin , Keski-Rahkonen Pekka , Sears Dorothy D. , Bonanni Bernardo , Gandini Sara , Johansson Harriet , Mellgren Gunnar 
Published: October 2023

Abstract
Background
Metformin reduces the incidence of breast cancer in patients with type 2 diabetes and obesity. However, our knowledge about the effects of metformin on cancer recurrence in breast tissue is limited. Therefore, in this study, we examined the breast tissue gene expression changes by metformin in breast cancer survivors.
Methods
Within the randomized placebo-controlled MetBreCS trial, baseline and one-year post-treatment fasting plasma and serum as well as breast tissue biopsies were collected. Breast cancer survivors with BMI >25 kg/m2 were randomly assigned to metformin (n=27), or placebo (n=13). We analyzed the transcriptomic profiles of the tissue biopsies by RNA sequencing. We also performed high-throughput metabolomics and sex steroid hormone analyses on the plasma and serum samples, respectively. To identify the metformin-associated signaling pathways in breast tissues, we integrated the gene expression and metabolomics and steroid hormone profiles using bivariate and functional analysis.
Results
Comparing breast tissue transcriptomic data, we identified MS4A1, HBA2, MT-RNR1 and MT-RNR2 expression to be differentially expressed in breast tissues from pre- and postmenopausal women. We also found significant metformin-associated down-regulation of EGFL6 and FDCSP in postmenopausal women. Long-term metformin treatment was significantly associated with decreased plasma levels of citrulline, arginine, PC ae C36:5, PC ae C38:6, caffeine, and 4-methyl-2-oxovalerate. The integration of transcriptomic and metabolomic profiles using bivariate correlation analysis followed by functional analysis revealed a down-regulation of immune response associated with the reduced plasma levels of arginine and citrulline in the pre- and postmenopausal metformin-treated group. The correlation between two steroid hormones (17β-estradiol, estrone) and global gene expression also showed an enrichment of steroid hormone biosynthesis and metabolism pathway with highly negatively correlated CYP11A1 and CYP1B1 expression in breast tissue from postmenopausal metformin-treated women.
Conclusions
Our results indicate that breast cancer survivors treated with metformin have specific changes in breast tissue gene expression that may prevent the development of new tumors. Reduced levels of circulating arginine, citrulline, and estrogens in metformin-treated breast cancer survivors may also contribute to reducing recurrence risk in obesity-associated breast cancer.
Trial registration
MetBreCs trial was started in 2015 and is registered at European Union Clinical Trials Register (EudraCT Protocol #: 2015-001001-14) on 7 October 2015.
The Perennial Logistical Challenges during Nigerian Elections: The Unmanned Aircraft System (UAS) Solution
Authors: Ehimare Ehinomen , Orikpete Ochuko , Ewim Daniel Raphael Ejike 
Published: October 2023

Abstract

The Independent National Electoral Commission (INEC) in Nigeria continues to grapple with recurrent logistical issues during elections. To address these challenges, this research focused on the potential deployment of a hybrid Vertical Take-off and Landing (VTOL) fixed-wing Unmanned Aircraft System (UAS), employing Commercial-off-the-Shelf (COTS) components and subsystems, as a conduit for efficient transmission of election results from remote polling units to nationwide collation centers. The study used a mixed-methods approach, combining technical evaluation, economic analysis, and a consideration of political and environmental contexts. Additionally, the research considered the practicalities of component sourcing and the economic implications of importing necessary equipment into Nigeria. Findings underscored the paramountcy of cost-effectiveness, component reliability, weight, and system criticality in the selection of COTS components that would best fulfill the project's objectives. Importantly, the results highlighted the potential political barriers to UAS deployment in this context. The study concludes that while the proposed UAS solution holds promise, it must contend with significant political hurdles and practical considerations concerning component sourcing and importation. The study recommends further exploration of these issues to optimally implement this technological solution to address Nigeria's election logistics challenges.
Irritable bowel syndrome associated with B. hominis or without relationship of it? A case control study and minireview
Authors: Maghsood Amir Hossein , Kayedimajd Shaghayegh , Motavallihaghi Seyedmousa , Abedian Rohallah , Kordi Shirafkan , Davoodi Lotfollah , Faizi Fariba , Soleymani Eissa 
Published: October 2023

Abstract
Background: Blastocystis hominis (B. hominis) is a protozoan parasite that is found worldwide distribution. some studies have suggested a link between B. hominis and the development of irritable bowel syndrome (IBS). The objective of this study was to determine the prevalence of B. hominis in patients with IBS compared to healthy individuals.
Material and methods: A total of 65 stool samples from patients with IBS and 65 samples from healthy individuals in northern Iran were examined, the samples were tested using various methods including direct smear, formalin ether sedimentation and culture to detect the presence of B. hominis. Additionally, polymerase chain reaction (PCR) was performed on all culture-positive isolates to confirm the results and identify the genotype.
Results: B. hominis was detected in 15.38% of IBS patients and 9.2% of the healthy group. The culture in RPMI1640 was found to be better than the formalin ether and direct smear methods. Positive samples were confirmed using the molecular method. No significant difference was observed in the order of B. hominisinfection between the two groups.
Conclusions: The results of our study indicate that no significant difference was observed in the order of B. hominisinfection between IBS patients and healthy groups. Therefore, firthet, study is necessary to determine the potential pathogenic effects of this parasite and its role in causing IBS.
Synergistic neuroprotective action of prolactin and 17β-estradiol on kainic acid-induced hippocampal injury and long-term memory deficit in ovariectomized rats.
Authors: Torre Karen De la , Cerbón Marco Antonio , Molina-Salinas Gladys , Suárez-Santiago José Eduardo , Morin Jean Pascal , Roldán-Roldán Gabriel , Picazo Ofir 
Published: October 2023

Abstract
Purpose
The neuroprotective actions of the ovarian hormone 17β-estradiol (E2) against different brain lesions have been continuously confirmed in a variety of models including kainic acid (KA) lesions. In the same line, the pituitary hormone prolactin (PRL), traditionally associated to lactogenesis, has recently been linked to a great diversity of functions, including neurogenesis, neuroprotection, and cognitive processes. While the mechanisms of actions of E2 regarding its neuroprotective and behavioral effects have been extensively explored, the molecular mechanisms of PRL related to these roles remain under investigation. The aim of the current study was to explore if the simultaneous administration of PRL and a low dose of E2 prevents the KA-induced cognitive deficit and if this action is associated to changes in hippocampal neuronal density.
Methods
Ovariectomized (OVX) rats were treated with saline, PRL and/or E2 in the presence or absence of KA. Neuroprotection was assessed by Nissl staining and neuron counting. Evaluation of memory was carried out by means of the novel object recognition test (NOR).
Results
These findings indicate that both PRL and E2 prevent short- and long-term memory deficits in lesioned animals. In addition, both hormones exert neuroprotection against KA-induced excitotoxicity in the hippocampus. Interestingly, the combined hormonal treatment was superior at improving the behavioral performance of rats in the NOR and neuronal survival than either treatment administered separately.
Conclusion
Taken together, these results suggest that these hormones act in different ways at the hippocampus to produce their behavioral, proliferative, and neuroprotective effects.
Alkalosis in Regional Citrate Anticoagulation during Continuous Renal Replacement Therapy—— A single-center retrospective study
Authors: Tang Hanqi , Cui Qinghong , Xu Shengyong , Wu Yao , Shi Jing , Zhu Huadong , Yu Xuezhong , Xu Jun 
Published: October 2023

Abstract
Background:
Metabolic alkalosis has raised concerns in patients receiving continuous renal replacement therapy (CRRT) via regional citrate anticoagulation (RCA). This study searched for alkalosis-related factors and mechanisms.
Measurements:
We conducted a retrospective cross-sectional study of alkalosis in patients who received CRRT for at least 12 hours with RCA at an emergency department in a tertiary hospital between April 2017 and April 2020.
Main Results:
The 59 patients meeting the inclusion criteria were 49% male, with a mean age of 55 ± 18 years old, and 42% had alkalosis by 12 hours after CRRT. Patients were divided into 4 groups based on whether they received NaHCO3 and alkalosis after 12 hours of CRRT. No significant differences in demographic features or laboratory results were observed among the groups. CRRT metrics, including blood flow rate, PBP rate, replacement fluid rate and total effluent rate, were significantly different among groups (p < 0.01). Multivariable logistic regression analysis indicated that the citrate rate was a risk factor for alkalosis (OR 1.088, 95% CI 1.020–1.161, p = 0.010). In patients receiving no NaHCO3 and without alkalosis, the linear regression analysis described the relationships of citrate with replacement fluid rate (citrate rate = 0.090 × replacement fluid rate + 56.581; R2 = 0.6918) and total effluent rate (citrate rate = 0.099 × total effluent rate + 2.449).
Conclusions:
This retrospective observational study demonstrated that CRRT metrics are highly associated with alkalosis after 12 hours of CRRT. Without NaHCO3 infusion, a 10-fold linear correlation was observed between citrate and total effluent rate in patients without metabolic alkalosis.