bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and propagation of bovine blood‐derived macrophages using a mixed culture with bovine endothelial B46 cells
Authors: Hiramatsu Kanae , Ikeda Rina , Kawaji Satoko , Ueno Yuichi , Nagata Reiko , Hayashi Ken‐Go , Iga Kosuke , Yoshioka Miyako , Takenouchi Takato 
Published: November 2023

AbstractMacrophages are innate immune cells with multiple functions such as phagocytosis, cytokine production, and antigen presentation. Since macrophages play critical roles in some bacterial infectious diseases in cattle, including tuberculosis, paratuberculosis, and brucellosis, the in vitro culturing of bovine macrophages is useful for evaluating host–pathogen interactions at the cellular and molecular levels. We have previously reported the establishment of two immortalized bovine liver sinusoidal cell lines, endothelial B46 cells and myofibroblast‐like A26 cells (Cell Biology International, 40, 1372−1379, 2016). In this study, we investigated the use of these cell lines as feeder cells that support the proliferation of bovine blood‐derived macrophages (BBMs). Notably, the B46 cell line efficiently acts as feeder cells for the propagation of BBMs. Compared with primary cultured vascular endothelial cells, the infinite proliferation ability of B46 cells is more beneficial for preparing confluent feeder layers. In conclusion, this study provides a simple and efficient protocol for the isolation and propagation of BBMs using a primary mixed culture of bovine whole blood with B46 feeder cells. Isolated BBMs are expected to be useful for developing in vitro models for studying the interactions between bovine pathogens and host immune cells.
A sensitive biosensor for cyanide detection using modified glassy carbon electrode with reduced graphene oxide and immobilization of horseradish peroxidase
Authors: Ghanavati Mahdi , Keshavarz Moraveji Mostafa , Iranshahi Davood 
Published: November 2023

AbstractCyanide is a highly poisonous and hazardous substance which may release into the environment from natural sources or industrial effluent; therefore, cyanide detection is a fundamental step to prevent environmental pollution and secure health and safety. In this study, we prepared a sensitive amperometric inhibition biosensor for cyanide detection by immobilization of horseradish peroxidase (HRP) enzyme and reduced graphene oxide (rGO) on the surface of glassy carbon electrode (GCE). To do so, we performed the amperometric measurement by modified GCE to test its efficiency in detecting cyanide. The optimum conditions of pH equal to 7.5, −100 mV applied potential, 0.7 μM mediator concentration, and 0.5 mM substrate concentration were found. Then, experiments were performed at different boundary conditions in a range of 0.1 to 10 μM cyanide concentration at optimal conditions and a low detection limit of 0.01 μM was obtained. Also, the possible mechanism of inhibition was analyzed based on the Michalis–Menten equation and non‐competitive inhibition was observed. Due to high sensitivity, low detection limit, and low cost, this biosensor is proposed as a useful method for cyanide determination in real samples.
Pattern of ventral temporal lobe interconnections in rhesus macaques
Authors: Bautista Julied , García‐Cabezas Miguel Á. , Medalla Maria , Rosene Douglas L. , Zikopoulos Basilis , Barbas Helen 
Published: November 2023

AbstractThe entorhinal cortex (EC, A28) is linked through reciprocal pathways with nearby perirhinal and visual, auditory, and multimodal association cortices in the temporal lobe, in pathways associated with the flow of information for memory processing. The density and laminar organization of these pathways is not well understood in primates. We studied interconnections within the ventral temporal lobe in young adult rhesus monkeys of both sexes with the aid of neural tracers injected in temporal areas (Ts1, Ts2, TE1, area 36, temporal polar area TPro, and area 28) to determine the density and laminar distribution of projection neurons within the temporal lobe. These temporal areas can be categorized into three different cortical types based on their laminar architecture: the sensory association areas Ts1, Ts2, and TE1 have six layers (eulaminate); the perirhinal limbic areas TPro and area 36 have an incipient layer IV (dysgranular); and area 28 lacks layer IV (agranular). We found that (1) temporal areas that are similar in laminar architecture by cortical type are strongly interconnected, and (2) the laminar pattern of connections is dependent on the difference in cortical laminar structure between linked areas. Thus, agranular A28 is more strongly connected with other agranular/dysgranular areas than with eulaminate cortices. Further, A28 predominantly projected via feedback‐like pathways that originated in the deep layers, and received feedforward‐like projections from areas of greater laminar differentiation, which emanated from the upper layers. Our results are consistent with the Structural Model, which relates the density and laminar distribution of connections to the relationship of the laminar structure between the linked areas. These connections were viewed in the context of the inhibitory microenvironment of A28, which is the key recipient of pathways from the cortex and of the output of hippocampus. Our findings revealed a higher population of calretinin (CR)‐expressing neurons in EC, with a significantly higher density in its lateral division. Medial EC had a higher density of CR neurons in the deep layers, particularly in layer Va. In contrast, parvalbumin (PV) neurons were more densely distributed in the deep layers of the lateral subdivisions of rostral EC, especially in layer Va, whereas the densities of calbindin (CB) neurons in the medial and lateral EC were comparable in all layers, except for layer IIIa, in which medial EC had a higher CB population than the lateral. The pattern of connections in the inhibitory microenvironment of EC, which sends and receives input from the hippocampus, may shed light on signal propagation in this network associated with diverse aspects of memory, and disruptions in neurologic and psychiatric diseases that affect this region.
Pragmatic Approach to Applying Polygenic Risk Scores to Diverse Populations
Authors: Patel Aniruddh P. , Fahed Akl C. 
Published: November 2023

AbstractPolygenic risk scores (PRS) estimate genetic susceptibility of an individual to disease and have the potential of providing utility in multiple clinical contexts. However, their performance, computation, and reporting in diverse populations remain challenging. Here, we present a pragmatic approach to optimize a PRS for a population of interest that leverages publicly available data and methods and consists of seven steps that are easily implemented without the requirement of expertise in complex genetics: step 1, selecting source genome‐wide association studies (GWAS) and imputation; step 2, selecting methods to compute polygenic score; step 3, adjusting scores using principal components of genetic ancestry; step 4, selecting the best performing score; step 5, defining percentiles of a population distribution; step 6, validating performance of the optimized polygenic score; and step 7, implementing the optimized polygenic score in clinical practice. © 2023 Wiley Periodicals LLC.
The effect of corporate social responsibility on firm performance: Insights from ISO 9001‐certified manufacturing firms in Ghana
Authors: Gyekye Kwabena Agyarko , Agana Joseph Akadeagre , Mireku Kwame , Domeher Daniel , Koranteng Eric Tieku 
Published: November 2023

AbstractThe purpose of this paper is to examine the effect of corporate social responsibility (CSR) on the performance of ISO 9001‐certified manufacturing firms in Ghana. Specifically, we explore the effect of CSR on the market value and profitability of manufacturing firms in Ghana that have adopted ISO 9001. We use panel data estimation techniques to examine the empirical link between CSR activities and the performance of ISO 9001 certified manufacturing firms. Our panel data was pooled from a total of 65 ISO 9001‐certified manufacturing firms in Ghana observed over 5 years yielding a total of 325 firm‐year observations. Our empirical results show that there is a positive relationship between CSR, market share and profitability of ISO 9001‐certified manufacturing firms. The results are indicative that firms stand to gain if they adopt a more deliberate strategy toward CSR activities. This study provides significant input to the extant literature and offers a comprehensive guide to policy makers and management of ISO 9001‐certified manufacturing firms on CSR engagement in emerging economies like Ghana.
UAV‐SHUTTLE‐MMM: UAV‐shuttle‐enabled mobility management model in sparsely deployed flying ad hoc networks
Authors: Agrawal Juhi , Kapoor Monit 
Published: November 2023

SummaryThe reliable surveillance of a large region typically needs a large number of unmanned aerial vehicles (UAVs), which leads to high operational costs in flying ad hoc networks (FANETs). Therefore, the UAV mobility model plays an important role that affecting the performance of FANET. This research paper comes up with a novel UAV‐shuttle‐enabled mobility management model (US‐MMM) that is a crucial attribute for the large and highly sparse network. The proposed mobility model uses UAV shuttles. A relatively lesser number of UAVs are used than comparative approaches to enhance the connectivity among the UAVs in a sparse network. UAV shuttle is the special self‐governing node, which traverses the network and collects the data to deliver to the base station (BS). The main strength of the proposed mobility model is the self‐governing ability of the UAV shuttle, which leads to high connectivity in the network. The UAV shuttle executes the proposed dynamic path planning algorithm and uses the adaptive ranking algorithm to determine its target. The main contribution of this research work is to develop a subnet scan and Number of Neighbor and Distance‐based Adaptive Ranking of Targets (NN‐DART) algorithm for dynamic route planning of UAV shuttle. The proposed mobility model is used with various routing schemes to check the difference in the performance of various routing schemes. The proposed mobility model is simulated in NS‐2.35, and the results have shown an improvement in the packet delivery ratio, routing overhead, and end‐to‐end delay.
Medicinal polypharmacology: Exploration and exploitation of the polypharmacolome in modern drug development
Authors: Stefan Sven Marcel , Rafehi Muhammad 
Published: November 2023

AbstractAt the core of complex and multifactorial human diseases, such as cancer, metabolic syndrome, or neurodegeneration, are multiple players that cross‐talk in robust biological networks which are intrinsically resilient to alterations. These multifactorial diseases are characterized by sophisticated feedback mechanisms which manifest cellular imbalance and resistance to drug therapy. By adhering to the specificity paradigm (“one target‐one drug concept”), research focused for many years on drugs with very narrow mechanisms of action. This narrow focus promoted therapy ineffectiveness and resistance. However, modern drug discovery has evolved over the last years, increasingly emphasizing integral strategies for the development of clinically effective drugs. These integral strategies include the controlled engagement of multiple targets to overcome therapy resistance. Apart from the additive or even synergistic effects in therapy, multitarget drugs harbor molecular‐structural attributes to explore orphan targets of which intrinsic substrates/physiological role(s) and/or modulators are unknown for future therapy purposes. We designated this multidisciplinary and translational research field between medicinal chemistry, chemical biology, and molecular pharmacology as ‘medicinal polypharmacology’. Medicinal polypharmacology emerged as alternative approach to common single‐targeted pharmacology stretching from basic drug and target identification processes to clinical evaluation of multitarget drugs, and the exploration and exploitation of the ‘polypharmacolome’ is at the forefront of modern drug development research.
Optimizing Sex Ratios of Hyalella azteca to Reduce Variability in Reproduction and Improve Reproductive Toxicity Test Methods
Authors: Khan H. N. , Bartlett A. J. , Kudla Y. M. , Prosser R. S. 
Published: November 2023

Abstract
Standard toxicological test methods with Hyalella azteca incorporate both lethal and sublethal (growth, reproduction) endpoints, although lethal endpoints are often favored in routine testing. However, sublethal endpoints are important to consider because they are ecologically relevant and are often more sensitive than lethality. It is difficult to achieve robust data for reproduction in H. azteca because high biological variability is associated with reproductive yield, likely due to complex interactions in mate selection, which can add to the variability in brood sizes that females produce. In addition, effects on reproduction often co-occur with effects on growth, making it difficult to differentiate effects on growth from those on reproduction in standard tests initiated with juveniles. The present study characterized the reproductive capacity of H. azteca by investigating the role of sex ratios in reproductive yield. Experiments were initiated in the absence of toxicants with sexually mature (6–8-week-old) individuals that were placed in different female-to-male ratios (1:1, 2:3, 3:2, and 7:3). Reproduction was monitored during weekly static renewals for 7 weeks. The results indicated that a higher female:male ratio (seven females to three males) improved reproductive success and lowered biological variability in reproduction. In addition, the body lengths and weights of newly mature amphipods were used to create a size distribution to aid in the identification of small female amphipods. Based on the results of our study, a novel reproductive toxicity test method is proposed that begins with sexually mature amphipods in a ratio of seven females to three males to minimize reproductive variability in amphipods and to enable a more effective assessment of contaminants using reproduction as a highly sensitive endpoint. However, evaluation of this novel method with toxicants is needed to demonstrate that results are comparable to standard methods. Environ Toxicol Chem 2024;43:712–722. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Evaluation criteria for artificial intelligence
Authors: Montrosse‐Moorhead Bianca 
Published: November 2023

AbstractCriteria identify and define the aspects on which what we evaluate is judged and play a central role in evaluation practice. While work on the use of AI in evaluation is burgeoning, at the time of writing, a set of criteria to consider in evaluating the use of AI in evaluation has not been proposed. As a first step in this direction, Teasdale's Criteria Domains Framework was used as the lens through which to critically read articles included in this special issue. This resulted in the identification of eight criteria domains for evaluating the use of AI in evaluation. Three of these criteria domains relate to the conceptualization and implementation of AI in evaluation practice. Five criteria domains are focused on outcomes, specifically those stemming from the use of AI in evaluation. More work is needed to further identify and deliberate possible criteria domains for AI use in evaluation.
Soil organic carbon stocks and belowground biomass in patches in heterogeneous grassland
Authors: Komainda Martin , Mohn Eliana , Kajzrová Klára , Obermeyer Kilian , Titěra Jan , Pavlů Vilém , Isselstein Johannes 
Published: November 2023

AbstractBackgroundSelective grazing creates stable patches of contrasting sward height, thereby providing different growth conditions for the grass sward above and below ground and potentially affecting soil organic carbon (SOC) stocks. We hypothesized that the presence of patches leads to greater spatial variability in belowground biomass (BGB) and SOC stocks than occurs between pastures managed under different stocking intensities.MethodsA long‐term grazing experiment consisting of three stocking intensities was used for this study. We studied BGB, SOC, and soil total nitrogen (Ntot) stocks in the 0–15 cm soil depth. Shannon diversity of plant species, soil bulk density, soil phosphorus, potassium, and magnesium contents were considered.ResultsThere were no significant effects of patch or stocking intensity on BGB, SOC, and Ntot stocks. Short patches had a greater Shannon diversity than tall patches (p < 0.05) and plant‐available nutrients in soil correlated positively with sward height (p < 0.05).ConclusionsWe conclude from the current results and previous studies that higher plant species diversity with lower soil nutrient contents in short‐patch areas and higher nutrient contents together with light competition in tall‐patch areas might balance each other out with respect to BGB and SOC stocks.
What is typical: Atypical in young children's attention regulation?: Characterizing the developmental spectrum with the Multidimensional Assessment Profiles—Attention Regulation Infant‐Toddler (MAPS‐AR‐IT) Scale
Authors: Nili Amanda N. , Miller Meghan , Zhang Yudong , Sherlock Philip R. , Burns James L. , Zola Anne , Kaat Aaron , Wakschlag Laurie S. , Krogh‐Jespersen Sheila 
Published: November 2023

AbstractWhile attention dysregulation is a promising early indicator of neurodevelopmental risk, in particular attention‐deficit/hyperactivity disorder (ADHD), it is difficult to characterize clinical concern due to its developmental expectability at the transition to toddlerhood. Thus, explicating the typical:atypical continuum of risk indicators is among the key future directions for research to promote early identification and intervention, and prevent decrements in the attainment of developmental milestones into early childhood. In this paper, we present the Multidimensional Assessment Profiles—Attention Regulation Infant‐Toddler (MAPS‐AR‐IT) Scale, a novel parent‐report survey of dimensional, developmentally specified indicators of attention (dys)regulation. Item Response Theory was employed to characterize the typical:atypical spectrum of both normative and more concerning dysregulation (including the contexts in which behavior occurs). We provide evidence of the validity of this measure in capturing the full typical:atypical spectrum via a longitudinal sample of typically developing children at 12–18 months of age (baseline) via concurrent scores on well‐validated temperament and clinical measures. We also examine longitudinal stability and predictive validity if the MAPS‐AR‐IT via a clinical interview of ADHD symptoms at 24–30 months (follow‐up). While not diagnostic, we present evidence of the utility of the MAPS‐AR‐IT in explicating individual neurodevelopmental risk and elucidating the broader typicality of behaviors related to attention (dys)regulation.
Front Cover
Authors: Juhong Aniwat , Li Bo , Liu Yifan , Yao Cheng‐You , Yang Chia‐Wei , Agnew Dalen W. , Lei Yu Leo , Luker Gary D. , Bumpers Harvey , Huang Xuefei , Piyawattanametha Wibool , Qiu Zhen 
Published: November 2023

A deep learning model based on hybrid convolutional and recurrent neural networks is proposed for generating sequential optoacoustic, unmixed optoacoustic of a specific contrast agent, and ultrasound images in order to extend the stack of crosssectional images and reduce acquisition time by approximately 71%.For further details please visit the article by Aniwat Juhong, Bo Li, Yifan Liu, Cheng‐You Yao, Chia‐Wei Yang, Dalen W. Agnew, Yu Leo Lei, Gary D. Luker, Harvey Bumpers, Xuefei Huang, Wibool Piyawattanametha, and Zhen Qiu (e202300142).image
Inside Cover
Authors: Li Kerui , Wu Qijia , Feng Shiyu , Zhao Hongyou , Jin Wei , Qiu Haixia , Gu Ying , Chen Defu 
Published: November 2023

This study explored the feasibility of diffuse reflectance spectroscopy (DRS) for discrimination between brain cancer and noncancer tissues. Our method could achieve a sensitivity of 93% and specificity of 95% for discriminating high‐grade glioma from normal white matter. Our results showed that DRS has the potential to be used for label‐free, real‐time in vivo cancer detection during brain surgery.For further details please visit the article by Kerui Li, Qijia Wu, Shiyu Feng, Hongyou Zhao, Wei Jin, Haixia Qiu, Ying Gu, and Defu Chen (e202300195).image
Targeting altered calcium homeostasis and uncoupling protein‐2 promotes sensitivity in drug‐resistant breast cancer cells
Authors: Balakrishnan Pavithra , Arasu Ashok , Velusamy Thirunavukkarasu 
Published: November 2023

AbstractMetastatic breast cancer has the highest mortality rate among women owing to its poor clinical outcomes. Metastatic tumors pose challenges for treatment through conventional surgery or radiotherapy because of their diverse organ localization and resistance to various cytotoxic agents. Chemoresistance is a significant obstacle to effective breast cancer treatment owing to cancer's heterogeneous nature. Abnormalities in intracellular calcium signaling, coupled with altered mitochondrial metabolism, play a significant role in facilitating drug resistance and contribute to therapy resistance. Uncoupling protein‐2 (UCP2) is considered as a marker of chemoresistance and is believed to play a major role in promoting metabolic shifts and tumor metastasis. In this context, it is imperative to understand the roles of altered calcium signaling and metabolic switching in the development of chemotherapeutic resistance. This study investigates the roles of UCP2 and intracellular calcium signaling (Ca2+) in promoting chemoresistance against cisplatin. Additionally, we explored the effectiveness of combining genipin (GP, a compound that reverses UCP2‐mediated chemoresistance) and thapsigargin (TG, a calcium signaling modulator) in treating highly metastatic breast cancers. Our findings indicate that both aberrant Ca2+ signaling and metabolic shifts in cancer cells contribute to developing drug‐resistant phenotypes, and the combination treatment of GP and TG significantly enhances drug sensitivity in these cells. Collectively, our study underscores the potential of these drug combinations as an effective approach to overcome drug resistance in chemoresistant cancers.
Stakeholder influences on management control systems for ESG governance and reporting in the global automotive industry
Authors: Yeh Ting‐Tsen , Xiao Yuanzhang , Daniel Shirley J. 
Published: November 2023

AbstractDrawing on Simons’ levers of control, we develop a theoretical model to explore the influences of stakeholders (customers, suppliers, employees, regulators, communities, industry associations, and investors) on Management Control Systems for achieving strategic environmental, social, and governance (ESG) objectives. We then test the model using data from over 600 firms in the global automotive industry by linking the eleven largest automotive manufacturers with over 500 of their tier‐1 and tier‐2 suppliers and examining whether stakeholder‐relevant metrics impact supplier retention and whether management compensation is tied to ESG governance. We also examine whether stakeholder influences lead to higher ESG scores. We find that stakeholder‐related metrics for emissions, human rights, customer health, employee empowerment and industry associations are related to management compensation systems tied to ESG. We also find significant linkages between stakeholder influences and ESG scores.