bluuebunny/crossref_metadata_2025_split huggingface dataset:
Could there be a slower physiological healing process in vegan individuals?
Authors: Dias Laís Ferreira , Acosta‐Navarro Julio Cesar , da Silva Tuane Cassemiro 
Published: December 2023

AbstractThe article “Comparing Tattoo Removal Responses in Vegan and Omnivore Patients” by Fusano et al. investigates the clinical outcomes of Q‐switched laser tattoo removal in vegans and omnivores. The study reveals that vegans required more laser sessions, exhibited poorer clinical responses, and experienced extended healing periods compared to omnivores. When well‐planned vegetarian diets offer numerous health benefits. Unfortunately, the study does not assess the diets of the groups, nor does it address factors like hydration levels and prior skincare regimens, limiting the interpretation of results.
Hyaluronic acid filler‐induced vascular occlusion—Three case reports and overview of prevention and treatment
Authors: Wang Rong , Li Yukun , Li Zhaoxin , Yao Haifeng , Zhai Zhaohui 
Published: December 2023

AbstractBackgroundVascular occlusion induced by hyaluronic acid injections is rare, but can lead to severe adverse events, including necrosis, blindness, and cerebral infarction.ObjectiveThis study aims to explore methods of reducing the risk of complications such as embolism induced by hyaluronic acid injection, and to study the impact of comprehensive systematic treatment on the prognosis of patients with hyaluronic acid embolism.MethodsThe clinical data of three female patients with vascular occlusion due to hyaluronic acid injection was analyzed. Their median age was 26 years, with symptoms presenting 1–6 h postinjection. Hospital stays averaged 6 days. Two patients had ptosis, diplopia, and severe pain after injection of eyebrows. The other, who had a nose enhancement, experienced facial skin color changes and intense pain.ResultsTwo patients received comprehensive, systematic treatment based on injectable hyaluronidase. One patient self‐discharged after receiving injectable hyaluronidase, antispasmodic, and vasodilator treatment on the night of embolism and returned to the hospital 3 days later with worsening embolism symptoms and received symptomatic treatment again. Two patients who received comprehensive, systematic treatment based on injectable hyaluronidase showed significant improvement, while the patient who did not undergo systematic treatment left scars on the face.ConclusionVascular occlusion caused by hyaluronic acid facial filling is a severe adverse event, and timely, comprehensive, systematic treatment can effectively improve irreversible damage caused by thrombosis, and even cure it.
Medical device‐related pressure injuries in paediatric patients: An incidence study in a children's hospital
Authors: Başbakkal Zümrüt , Yilmaz Hatice Bal , Gümüş Merve , Belli Mustafa , Erçelik Zübeyde Ezgi 
Published: December 2023

AbstractAimTo determine the incidence of pressure injuries from medical devices in children.BackgroundMedical devices can cause pressure injuries on skin and soft tissues.DesignA prospective, descriptive study adhering to STROBE guidelines.MethodsThis study was conducted in the third‐level Paediatric Intensive Care Unit of Ege University Hospital in İzmir, Türkiye between April 2019 and October 2019 in Türkiye. Patients aged between 1 month and 18 years with medical devices were observed for pressure injuries using Braden scales and a specific monitoring form.ResultsIn this study, we followed 522 medical devices applied to 96 patients. The three most commonly used medical devices were the ECG probe (21%), the blood pressure cuff (16%) and the saturation probe. Out of the 522 medical devices followed, 36 caused pressure injuries (6.8%).ConclusionThe incidence of medical device‐related pressure injuries was found to be high. Effective training and implementation strategies need to be devised for paediatric nurses to prevent pressure injuries associated with medical devices.Relevance to Clinical PracticeThe results of this study reveal that pressure injuries related to medical devices are an important health problem in paediatric hospitals. Therefore, awareness‐raising and educational activities among health professionals and nurses should be accelerated.Patient or Public ContributionNo patient or public contribution in the study.
Phenotypic and transcriptional features of Araliaceae species under distinct light environments
Authors: Niu Yu‐Qian , Zhang Yu‐Xin , Wang Xin‐Feng , Wen Jun , Wang Zhen‐Hui , Yang Ji , Wang Yu‐Guo , Zhang Wen‐Ju , Song Zhi‐Ping , Li Lin‐Feng 
Published: December 2023

AbstractElucidating how plant species respond to variable light conditions is essential to understanding the ecological adaptation to heterogeneous environments. Plant performance and gene regulatory network underpinning the adaptation have been well documented in heliophytic species. However, it remains largely unclear how the sciophytic plants respond to distinct light conditions. We measured phenotypic and transcriptomic features of four sciophytic (Fatsia japonica, Metapanax delavayi, Heptapleurum arboricola, and Heptapleurum delavayi) and one heliophytic woody species (Tetrapanax papyrifer) of the Araliaceae family under distinct light conditions. Our phenotypic comparisons demonstrate that the four sciophytic species maintain similar photosynthesis efficiency between high light and low light conditions. However, a significantly decreased photosynthesis rate was observed under the low light conditions of the heliophytic species compared with the high light conditions. In addition, our leaf anatomical analyses revealed that, while all five species showed different anatomical structures under distinct light conditions, the sciophytic species possessed a lower degree of phenotypic plasticity relative to the heliophytic species. Further comparisons of the transcriptome profiling showed that differentially expressed genes identified in the five species were functionally related to photosynthesis, secondary metabolites, and other basic metabolisms. In particular, differential regulation of the photosynthesis‐related and photomorphogenesis‐related genes were potentially correlated with the phenotypic responses to the distinct light conditions of the five species. Our study provides evolutionary and ecological perspectives on how the heliophytic and sciophytic woody species respond to shade and sunlight environments.
Evaluation of the clinical outcome of hypercalcemia of malignancy and concurrent azotemia in dogs with lymphoma
Authors: Strumpf Alyssa A. , Selmic Laura , Husbands Brian 
Published: December 2023

AbstractBackgroundHypercalcemia of malignancy (HM) secondary to lymphoma in dogs has the potential to cause renal injury.Hypothesis/ObjectivesCharacterize outcomes related to acute kidney injury (AKI) secondary to HM. We hypothesized that dogs do suffer AKI regardless of HM severity at the time of lymphoma diagnosis or relapse.AnimalsRetrospective study. Twenty‐nine dogs with lymphoma, HM, and azotemia (International Renal Interest Society [IRIS] grade II or higher AKI) that underwent chemotherapy were identified at 2 veterinary institutions.MethodsLogistic regression and descriptive statistical analysis were performed to evaluate data for potential prognostic factors.ResultsAfter initiating treatment, resolution of hypercalcemia and azotemia occurred in 100% (29/29) and 79.3% (23/29) of dogs, respectively. Resolution of azotemia was influenced by serum creatinine concentration (odds ratio [OR], 0.148; Confidence interval [CI], 0.03‐0.734; P = .02) and total hypercalcemia (OR, 0.36; CI, 0.14‐0.93; P = .04) at diagnosis, whereas blood urea nitrogen concentration, IRIS grade, sex, and whether or not dogs were hospitalized were not significant factors. At data analysis, 13.8% (4/29) of dogs were alive or lost to follow‐up. Of those dead, 4 dogs (15%) had renal disease at the time of death, 2/4 having concurrent lymphoma progression.Conclusions and Clinical ImportanceAlthough AKI may be of clinical concern in dogs with HM secondary to lymphoma at diagnosis, death secondary to renal impairment appears to be infrequent.
Plastic and genomic change of a newly established lizard population following a founder event
Authors: Sabolić Iva , Mira Óscar , Brandt Débora Y. C. , Lisičić Duje , Stapley Jessica , Novosolov Maria , Bakarić Robert , Cizelj Ivan , Glogoški Marko , Hudina Tomislav , Taverne Maxime , Allentoft Morten E. , Nielsen Rasmus , Herrel Anthony , Štambuk Anamaria 
Published: December 2023

AbstractUnderstanding how phenotypic divergence arises among natural populations remains one of the major goals in evolutionary biology. As part of competitive exclusion experiment conducted in 1971, 10 individuals of Italian wall lizard (Podarcis siculus (Rafinesque‐Schmaltz, 1810)) were transplanted from Pod Kopište Island to the nearby island of Pod Mrčaru (Adriatic Sea). Merely 35 years after the introduction, the newly established population on Pod Mrčaru Island had shifted their diet from predominantly insectivorous towards omnivorous and changed significantly in a range of morphological, behavioural, physiological and ecological characteristics. Here, we combine genomic and quantitative genetic approaches to determine the relative roles of genetic adaptation and phenotypic plasticity in driving this rapid phenotypic shift. Our results show genome‐wide genetic differentiation between ancestral and transplanted population, with weak genetic erosion on Pod Mrčaru Island. Adaptive processes following the founder event are indicated by highly differentiated genomic loci associating with ecologically relevant phenotypic traits, and/or having a putatively adaptive role across multiple lizard populations. Diverged traits related to head size and shape or bite force showed moderate heritability in a crossing experiment, but between‐population differences in these traits did not persist in a common garden environment. Our results confirm the existence of sufficient additive genetic variance for traits to evolve under selection while also demonstrating that phenotypic plasticity and/or genotype by environment interactions are the main drivers of population differentiation at this early evolutionary stage.
Disruption of a Plasmodium falciparum patatin‐like phospholipase delays male gametocyte exflagellation
Authors: Pietsch Emma , Niedermüller Korbinian , Andrews Mia , Meyer Britta S. , Lenz Tobias L. , Wilson Danny W. , Gilberger Tim‐Wolf , Burda Paul‐Christian 
Published: December 2023

AbstractAn essential process in transmission of the malaria parasite to the Anopheles vector is the conversion of mature gametocytes into gametes within the mosquito gut, where they egress from the red blood cell (RBC). During egress, male gametocytes undergo exflagellation, leading to the formation of eight haploid motile microgametes, while female gametes retain their spherical shape. Gametocyte egress depends on sequential disruption of the parasitophorous vacuole membrane and the host cell membrane. In other life cycle stages of the malaria parasite, phospholipases have been implicated in membrane disruption processes during egress, however their importance for gametocyte egress is relatively unknown. Here, we performed comprehensive functional analyses of six putative phospholipases for their role during development and egress of Plasmodium falciparum gametocytes. We localize two of them, the prodrug activation and resistance esterase (PF3D7_0709700) and the lysophospholipase 1 (PF3D7_1476700), to the parasite plasma membrane. Subsequently, we show that disruption of most of the studied phospholipase genes does neither affect gametocyte development nor egress. The exception is the putative patatin‐like phospholipase 3 (PF3D7_0924000), whose gene deletion leads to a delay in male gametocyte exflagellation, indicating an important, albeit not essential, role of this enzyme in male gametogenesis.
Epidemiological and CBCT characterizations of odontomas: A retrospective study of 87,590 subjects
Authors: Liu Shu , Lin Zitong , Wen Shanhui , Teng Yuehui , Xie Kaipeng , Huang Yanhua 
Published: December 2023

AbstractObjectivesThis study aimed to assess the epidemiological and three‐dimensional (3D) radiological characterizations of odontomas, as well as the spatial relationship between odontomas and gubernaculum tracts (GT).Materials and MethodsWe retrieved the cone‐beam computed tomography (CBCT) data of 87,590 patients. Dentition, location, type, diameter of the odontomas, width of the dental follicle (DF), the spatial relationship between the odontoma and GT, and the influence on adjacent teeth were evaluated.ResultsSignificant differences were found in age, dentition, location, Max/Min diameter, width of DF, impaction, retention, and root bending of adjacent teeth among different spatial relationships between the odontoma and GT (all p < 0.05), as well as in age, type and size, absence, impaction, malposition, and retention of adjacent teeth among different locations of odontomas (all p < 0.05). Compared to the odontomas without impaction, those with impaction had larger diameter (p < 0.05 in all directions). This statistically significant association was consistent for odontomas with malposition, while no similar result was observed in the maximum diameter.ConclusionOur findings provide the preliminary data for clinicians to comprehensively understand the incidence, radiographic characterizations and symptoms of odontoma in Chinese population.
Retronychia in children: A case series
Authors: Díaz María Sol , Boggio Paula , Calvano Roberta , Scacchi María Florencia , Giachetti Ana 
Published: December 2023

AbstractBackgroundRetronychia is characterized by proximal ingrowing of the nail plate into the proximal nail fold. It is always associated with the presence of two or more overlapping nail plates under the proximal nail fold, clinical signs of chronic proximal paronychia refractory to antimicrobial treatment, and a yellowish nail that does not grow. It mainly affects young female adults, with less than 30 pediatric cases described in the literature so far.MethodsRetrospective and observational study of patients between 0 and 18 years with a clinical and/or ultrasound diagnosis of retronychia attending a pediatric dermatology service between December 2020 and January 2022.ResultsWe identified 9 patients with retronychia, 7 girls and 2 boys. In all cases, the hallux nails were affected with 5 unilateral and 4 bilateral cases. On physical examination we observed the following signs: thickened and opaque nail plate (one patient), yellowish nail plate (7 patients), double nail plate (6 patients), and erythema with edema, pain, and suppuration of the proximal nail fold (7 patients). Ultrasound was performed in 7 patients and specific findings of retronychia were found in 5 of them. All patients received topical treatment and were referred for the appropriate surgical treatment. To date, only one patient underwent nail avulsion, which was followed by complete recovery.ConclusionsRetronychia is underdiagnosed, particularly in the pediatric population. We present a series of 9 cases of retronychia in children, with clinical and ultrasonographic findings consistent with those of adults. We emphasize the importance of recognizing this entity, which will allow early and adequate treatment.
Streptozotocin induced hyperglycaemia in the invertebrate diabetic model Bombyx mori
Authors: Ankola Kunal , Chiome Tafadzwa Justin , Srinivasan Asha , H. B. Manjunatha 
Published: December 2023

AbstractWorldwide spread in the incidence of diabetes justified the need of extensive research in an appropriate model system to develop an effective treatment strategy for diabetology. In recent years, because of animal ethical issues and limitations, the silkworm (Bombyx mori) has emerged as a perfect invertebrate model for various biomedical experiments. However, there are several issues related to induce hyperglycaemia in the mammalian system that have not been studied in the silkworms. In the present study, we investigated the consequences of induced hyperglycaemia by oral and intravascular administration of streptozotocin (STZ) and glucose in the silkworms. Interestingly, results confirmed the competence of STZ to induce hyperglycaemia when administered through intravascular route, but suppress the glucose concentration if administrated orally in the silkworms. Comparatively, the severity of induced hyperglycaemia in the silkworms remained nearly the same in response to the oral and intravascular administration of glucose. Furthermore, prolonged administration of STZ has recorded negative effect on the growth rate of the silkworms, whereas a drastic decline in the same was reported in the silkworms administered with glucose. Notably, these results confirm that the response shown by the silkworms to STZ is very similar to that of mammals, making it an excellent model to study diabetic complications and consequences associated with STZ in diabetology.
Decoding the guardians of cotton resilience: A comprehensive exploration of the βCA genes and its role in Verticillium dahliae resistance
Authors: Yang Mengying , Umer Muhammad Jawad , Wang Heng , Han Jiale , Han Jiangping , Liu Qiankun , Zheng Jie , Cai Xiaoyan , Hou Yuqing , Xu Yanchao , Wang Yuhong , Khan Muhammad Kashif Riaz , Ditta Allah , Liu Fang , Zhou Zhongli 
Published: December 2023

AbstractPlant Carbonic anhydrases (Cas) have been shown to be stress‐responsive enzymes that may play a role in adapting to adverse conditions. Cotton is a significant economic crop in China, with upland cotton (Gossypium hirsutum) being the most widely cultivated species. We conducted genome‐wide identification of the βCA gene in six cotton species and preliminary analysis of the βCA gene in upland cotton. In total, 73 βCA genes from six cotton species were identified, with phylogenetic analysis dividing them into five subgroups. GHβCA proteins were predominantly localized in the chloroplast and cytoplasm. The genes exhibited conserved motifs, with motifs 1, 2, and 3 being prominent. GHβCA genes were unevenly distributed across chromosomes and were associated with stress‐responsive cis‐regulatory elements, including those responding to light, MeJA, salicylic acid, abscisic acid, cell cycle regulation, and defence/stress. Expression analysis indicated that GHβCA6, GHβCA7, GHβCA10, GHβCA15, and GHβCA16 were highly expressed under various abiotic stress conditions, whereas GHβCA3, GHβCA9, GHβCA10, and GHβCA18 had higher expression patterns under Verticillium dahliae infection at different time intervals. In Gossypium thurberi, GthβCA1, GthβCA2, and GthβCA4 showed elevated expression across stress conditions and tissues. Silencing GHβCA10 through VIGS increased Verticillium wilt severity and reduced lignin deposition compared to non‐silenced plants. GHβCA10 is crucial for cotton's defense against Verticillium dahliae. Further research is needed to understand the underlying mechanisms and develop strategies to enhance resistance against Verticillium wilt.
Electroporation‐based delivery of proteins in Penium margaritaceum and other zygnematophycean algae
Authors: Carrillo‐Carrasco Vanessa Polet , Hernández‐García Jorge , Weijers Dolf 
Published: December 2023

AbstractZygnematophycean algae represent the streptophyte group identified as the closest sister clade to land plants. Their phylogenetic position and growing genomic resources make these freshwater algae attractive models for evolutionary studies in the context of plant terrestrialization. However, available genetic transformation protocols are limited and exclusively DNA‐based. To expand the zygnematophycean toolkit, we developed a DNA‐free method for protein delivery into intact cells using electroporation. We use confocal microscopy coupled with fluorescence lifetime imaging to assess the delivery of mNeonGreen into algal cells. We optimized the method to obtain high efficiency of delivery and cell recovery after electroporation in two strains of Penium margaritaceum and show that the experimental setup can also be used to deliver proteins in other zygnematophycean species such as Closterium peracerosum‐strigosum‐littorale complex and Mesotaenium endlicherianum. We discuss the possible applications of this proof‐of‐concept method.
Target of rapamycin (TOR) plays a role in regulating ROS‐induced chloroplast damage during cucumber (Cucumis sativus) leaf senescence
Authors: Ma Lifeng , Song Tiefeng , Yu Yongbo , Liu Linghao , Qu Mengqi , Zhou Shuang , Meng Xiangnan , Fan Haiyan 
Published: December 2023

AbstractIn cucumber production, delaying leaf senescence is crucial for improving cucumber yield and quality. Target of rapamycin (TOR) is a highly conserved serine/threonine protein kinase in eukaryotes, which can integrate exogenous and endogenous signals (such as cell energy state levels) to stimulate cell growth, proliferation, and differentiation. However, no studies have yet examined the regulatory role of TOR signalling in cucumber leaf senescence. In this study, the effects of TOR signalling on dark‐induced cucumber leaf senescence were investigated using the TOR activator MHY1485 and inhibitor AZD8055 combined with transient transformation techniques. The results indicate that TOR responds to dark‐induced leaf senescence, and alterations in TOR activity/expression influence cucumber leaf resistance to dark‐induced senescence. Specifically, in plants with elevated TOR activity/expression, we observed reduced expression of senescence‐related genes, less membrane lipid damage, decreased cell apoptosis, lower levels of reactive oxygen species production, and less damage to the photosynthetic system compared to the control. In contrast, in plants with reduced TOR activity/expression, we observed higher expression of senescence‐related genes, increased membrane lipid damage, enhanced cell apoptosis, elevated levels of reactive oxygen species production, and more damage to the photosynthetic system. These comprehensive results underscore the critical role of TOR in regulating dark‐induced cucumber leaf senescence. These findings provide a foundation for controlling premature leaf senescence in cucumber production and offer insights for further exploration of leaf senescence mechanisms and the development of more effective control methods.