bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical risk factors and protective factors of preeclampsia in China
Authors: Hou Lei , Li Jieyan , Zhao Rong , Liu Liheng , Zhang Weiyuan , Zou Liying 
Published: January 2024

Abstract
Objective: The pathophysiology of preeclampsia remains unclear. Identification of risk factors and protective factors will help us to better elucidate the pathophysiologic basis of preeclampsia and predict it.
Methods: A cross sectional survey of all deliveries in 39 hospitals in 14 provinces of mainland China from January 1 to December 31 2011 was evaluated for risk factors and protective factors of preeclampsia.
Results: The incidence of preeclampsia was 3.7% (4137/110926), The results of multivariate logistic analysis showed that the main independent risk factors for preeclampsia included chronic hypertension[RR=9.190, 95%CI: (8.102-10.423)], assisted reproduction [OR=4.487, 95%CI:(2.591-7.772)], obesity [OR=3.822 , 95%CI: (3.556-4.107)], advanced age [OR=2.509(2.321-2.712)], GDM/DM [OR=1.656(1.479-1.855)], multiple pregnancy [OR=1.491, 95%CI: (1.283-1.735)], anemia [OR=1.253, 95%CI: (1.110-1.414)] and AB blood type [OR=1.131 , 95%CI: (1.008-1.269)]. Protective factors for preeclampsia included placenta previa [OR=0.401, 95%CI: (0.296-0.543)], A blood type [OR=0.885 , 95%CI: (0.816-0.959)] and height [OR=0.991, 95%CI: (0.985-0.997)]. The obstetrics complications associated with preeclampsia raised obviously, including preterm birth[RR=8.542 , 95%CI: (7.985-9.137)], FGR[RR= 5.020 , 95%CI: (4.635-5.437)], placenta abruption [RR=3.453, 95%CI: (2.777-4.293)]and oligohydramnios [RR=1.520, 95%CI: (1.306-1.768)].
Discussion: Our findings suggest chronic hypertension, assisted reproduction, obesity, advanced maternal age, GDM/DM, multiple pregnancy, anemia and AB blood type were the risk factors of preeclampsia. placenta previa, A blood type, height, and advanced educated degree were the protective factors for it.
Visual Analysis Method for Unmanned Pumping Stations on Dynamic Platforms based on Data Fusion Technology
Authors: Liu Zhen , Chen Sen , Zhang Zhaobo , Qin Jiahao , Peng Bao 
Published: January 2024

Abstract
As the scale of water conservancy projects continues to expand, the amount and complexity of analytical data have also correspondingly increased. Currently, As the scale of water conservancy projects continues to expand, the amount and complexity of analytical data have also correspondingly increased. Currently, It is difficult to realize project management decision support based on a single data source, and most manual analysis methods not only have high labor costs, but also are prone to the risk of misjudgment and misjudgement, resulting in huge property losses. Based on this problem, this paper proposes a visualization and analysis method for unmanned pumping stations on a dynamic platform based on data fusion technology. First, the method uses the transfer learning method to make ResNet18 model obtain generalization ability. Secondly, the method uses the ResNet18 model to extract image features, and outputs fixed length sequence data as the input of long short-term memory (LSTM) model, Finally, the method uses the LSTM model outputs the classification results. The experimental results show that the algorithm model can achieve an accuracy of 99.032%, effectively recognize and classify pump station images, and reduce the occurrence of pump station accidents.
Anti-biofilm activity of antimicrobial peptide CRAMP-34 by regulating 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (KduD) of Escherichia coli
Authors: Xiong Jing , Yang Can , Liu Dan , Yang Hongzao , Long Jinying , Liang Yuan , Hu Huiming , Peng Lianci , Zhang Yang , Fu Lizhi , Yu Yuandi , Xu Dengfeng , Zhang Suhui , Qiu Jinjie , He Yuzhang , Chen Hongwei 
Published: January 2024

Abstract
Escherichia coli (E. coli) has high antimicrobial resistance and is globally prevalent, forming aggressive and dense bacterial biofilms. Previous studies have shown that the mouse antimicrobial peptide CRAMP-34 eradicates biofilms. The current study aimed to investigate the mechanism of CRAMP-34 on the biofilm of a clinically isolated E. coli (No. Ec032) with strong biofilm formation ability. An unreported gene kduD associated with Ec032 biofilm formation was identified by transposon mutation technology, and the kduD gene deletion strain Ec032ΔkduD was constructed using CRISPR/Cas9. Furthermore, the deletion of kduD gene reduced the motility of Ec032 and inhibited the aggregation of bacteria to form mature biofilms. Also, CRAMP-34 inhibited the motility of Ec032 and significantly cleared the mature biofilm. RT-qPCR showed that CRAMP-34 significantly downregulated the biofilm-related genes, including ycgR, papG, csgD, bcsA, uxaA, uxuA, kduD, and araE. These findings indicated that KduD protein is a potential target of CRAMP-34. Therefore, this study provides the foundation for the prevention and treatment of E. coli biofilm and also develops CRAMP-34 as a new biofilm scavenger.
Determining how oxygen legacy affects trajectories of soil denitrifier community structure, functional dynamics, and N2O emissions
Authors: Sennett Louise B. , Roco Constance A. , Lim Natalie Y. N. , Yavitt Joseph B. , Dörsch Peter , Bakken Lars R. , Shapleigh James P. , Frostegård Åsa 
Published: January 2024

Abstract

Denitrification – a key process in the global nitrogen cycle and main source of the greenhouse gas N2O – is intricately controlled by O2. While the transition from aerobic respiration to denitrification is well-studied, our understanding of denitrifier communities' responses to cyclic oxic/anoxic shifts, prevalent in natural and engineered systems, is limited. Here, agricultural soil was exposed to repeated cycles of long or short anoxic spells (LA; SA) or constant oxic conditions (Ox). Unexpectedly, Ox had up to three times greater denitrification and N2O reduction rates compared to LA and SA during a final anoxic incubation, despite comparable bacterial biomass and denitrification gene abundances. Metatranscriptomics indicated that LA favoured canonical denitrifiers carrying nosZ clade I. Ox instead favoured nosZ clade II-carrying partial- or non-denitrifiers, suggesting efficient partnering of the reduction steps among organisms. SA had the slowest denitrification progression and highest accumulation of intermediates, indicating less functional coordination. The findings demonstrate how adaptations of denitrifier communities to varying O2 conditions are tightly linked to the duration of oxic-anoxic cycles, emphasizing the importance of knowing an environment´s O2 legacy for accurately predicting N2O emissions originating from denitrification.
Fatty acid metabolism constrains Th9 cell differentiation and anti-tumor immunity via modulation of retinoic acid receptor signaling
Authors: Endo Yusuke , Nakajima Takahiro , Kanno Toshio , Ueda Yuki , Miyako Keisuke , Endo Takeru , Yokoyama Satoru , Asou Hikari , Yamada Kazuko , Ikeda Kazutaka , Togashi Yosuke 
Published: January 2024

Abstract

T helper 9 cells (Th9) are interleukin 9 (IL-9)–producing cells that have diverse functions ranging from anti-tumor immune responses to driving allergic inflammation. Th9 cells differentiate from naïve CD4+ T cells in the presence of IL-4 and transforming growth factor-beta (TGF-β); however, our understanding of the molecular basis of their differentiation remains incomplete. Previously, we reported that the differentiation of another subset of TGF-β–driven T helper cells, Th17 cells, is highly dependent on de novo lipid biosynthesis. On the basis of this finding, we hypothesized that lipid metabolism may also be important for Th9 cell differentiation. We therefore investigated the differentiation and function of mouse and human Th9 cells in vitro under conditions of pharmacologically or genetically induced deficiency of intracellular fatty acid content and in vivo in mice genetically deficient for acetyl-CoA carboxylase 1 (ACC1), an important enzyme for fatty acid biosynthesis. Both inhibition of de novo fatty acid biosynthesis and deprivation of environmental lipids augmented differentiation and IL-9 production in mouse and human Th9 cells. Mechanistic studies revealed that the augmentation of Th9 cell differentiation was mediated by retinoic acid receptor and the TGF-β–SMAD signaling pathways. Upon adoptive transfer, ACC1-inhibited Th9 cells suppressed tumor growth in murine models of melanoma and adenocarcinoma. Together, our findings highlight a novel role of fatty acid metabolism in controlling the differentiation and in vivo functions of Th9 cells.
Baculovirus and plasmid vector-mediated transgenic experiments in the embryonic cell cultures developed from the freshwater crustacean Daphnia magna
Authors: CP Sreevidya , Balakrishnan Soumya , Puthumana Jayesh 
Published: January 2024

Abstract

Cell culture represents an indispensable tool for investigating fundamental biological processes. Nevertheless, technical challenges such as low cell yield, sub-optimal cell differentiation, and inadequate attachment to the growth substrate have restricted the application of this tool in many studies. Here, we introduce an easy protocol for the preparation of primary cell cultures from Daphnia magna embryos, offering a versatile approach to address cell biological questions in conjunction with the robust in vivo model of D. magna. The development of transgenic cells is an emerging interdisciplinary field that can be used for the fundamental understanding of normal and pathological responses of cells and the improvement of tissue functionality. The application of this technology to primary cells is still in its infancy but promises to accelerate research. In this work, embryonic cell culture is developed from D. magna; and is used to standardize viral (BacIe1-GFP) and plasmid vector (pCS-EF1α1-DSRed2)-mediated transgenic experiments. The standardized conditions methodology for developing embryonic cell culture, Cellfectin-mediated transfection and baculovirus-mediated transduction methods envisage strengthening the crustacean cell line research and bringing forth the Daphnia cell culture system as a 'model' in vitro system for crustaceans. Additionally, the simplicity and flexibility of the methodology described are expected to lead to widespread use in many biological research areas, including their wide application to ecotoxicological and epigenetic studies which are currently limited to in vivo studies. This is the first report on the optimization of cell culture medium for freshwater crustaceans and the use of baculovirus for transduction studies in D. magna embryonic cell culture.
cellMarkerPipe: Cell Marker Identification and Evaluation Pipeline in Single Cell Transcriptomes
Authors: Yao Qiuming , Jia Yinglu , Ma Pengchong 
Published: January 2024

Abstract
Assessing marker genes from all cell clusters can be time-consuming and lack systematic strategy. Streamlining this process through a unified computational platform that automates identification and benchmarking will greatly enhance efficiency and ensure a fair evaluation. We therefore developed a novel computational platform, cellMarkerPipe (https://github.com/yao-laboratory/cellMarkerPipe), for automated cell-type specific marker gene identification from scRNA-seq data, coupled with comprehensive evaluation schema. CellMarkerPipe adaptively wraps around a collection of commonly used and state-of-the-art tools, including Seurat, COSG, SC3, SCMarker, COMET, and scGeneFit. From rigorously testing across diverse samples, we ascertain SCMarker’s overall reliable performance in single marker gene selection, with COSG showing commendable speed and comparable efficacy. Furthermore, we demonstrate the pivotal role of our approach in real-world medical datasets. This general and opensource pipeline stands as a significant advancement in streamlining cell marker gene identification and evaluation, fitting broad applications in the field of cellular biology and medical research.
Investigation of the Electrochemical Properties of Desert Rose-Like ZIF-67/NiCoTe Hybrid Composite as Asymmetric Supercapacitors for Energy Storage Applications
Authors: Meghanathan K. L. , Parthibavarman M. 
Published: January 2024

Abstract

The distinctive morphology of the desert rose structure facilitates enhanced accessibility to many electroactive sites, hence enhancing the internal electrical characteristics of the ZIF-67/NiCoTe composite. This allows for the effective utilization of the whole resource and enhances rapid redox response kinetics. The synthesis was achieved by a direct hydrothermal technique. The electrode materials that have been manufactured have exceptional electrochemical characteristics. The ZIF-67/NiCoTe material exhibits a specific capacitance of 2215 Fg− 1 when subjected to an imposed current density of 1 Ag− 1. In addition, it retains 97.4% of its original capacitance even after enduring 10000 cycles. In addition, the ZIF-67/NiCoTe//AC hybrid supercapacitor operates at a specific voltage of 1.5 V. The system has an energy density of 67.5 Wh kg− 1, with a corresponding power density of 2422.2 W kg− 1. Furthermore, it retains 93.5% of its capacitance even after enduring 10000 cycles at a significant current density of 10 Ag− 1. This research introduces a straightforward and efficient method for fabricating desert rose-shaped electrodes made of bimetallic nickel-cobalt telluride. These electrodes demonstrate excellent performance in hybrid supercapacitors.
Burnout, Associated Determinants and Effects among Nurses and Midwives at Selected CHAG Facilities in the Greater Accra Region, Ghana
Authors: Kyei Enoch , Takyi Sylvia Akpene 
Published: January 2024

Abstract
Background
Burnout among nurses and midwives is a public health concern due to its rapidly increasing prevalence. Although several studies have explored burnout among nurses in general, research specifically focusing on burnout among nurses and midwives in faith-based health facilities remains limited.
Objective
We investigated burnout, associated determinants and effects among nurses and midwives in selected CHAG facilities in the greater Accra region, Ghana.
Methods
The study recruited 266 nurses and midwives, using a structured questionnaire. Proportionate sampling technique was used to sample nurses and midwives from the selected CHAG facilities. The Maslach Burnout Inventory survey tool was used to assess data on the determinants and effects of burnouts among study participants. Next, Chi-square test and multivariate logistic regression was used to determine factors associated with burnout.
Results
The majority (78.6%) were females aged between 30–39 years. About a third of the nurses and midwives (34.22%) reported experiencing high level of burnout. In the multivariate logistic regression analysis, being Muslim [OR = 3.54; 95% CI: 1.08, 11.61; p = 0.037], working at the emergency department [OR = 2.93; 95% CI; 1.09, 7.92; p = 0.034], working at the paediatric ward [OR = 7.82; 95% CI: 1.65, 37.18; p = 0.010] and having items to work with more than half of the time [OR = 0.10; 95% CI: 0.04, 0.24; p = 0.000] were found to be associated with burnout among nurses and midwives. The effects of burnout included insomnia, irritability, sleep disorders, depression, cardiovascular disease, drug abuse, increased alcohol intake, absenteeism, and reduced productivity.
Conclusion
Nurses and midwives in the three selected CHAG facilities in Accra experienced high levels of burnout. In-service training on building good interpersonal relationship among nurses and midwives, adequate staffing and training on coping mechanism is encouraged.
Spatiotemporal changing pattern of land use and land cover of Sangu- Matamuhuri watershed in the South-Eastern part of Bangladesh
Authors: Rudra Ajit Kumar , Alam A. K. M. Rashidul 
Published: January 2024

Abstract

This study employed integrated remote sensing (RS) and geographic information system (GIS) approaches to characterize the spatiotemporal changing patterns of LULC in the upper part of Sangu-Matamuhuri Watershed (SMW) from 1988 to 2019 utilizing Landsat imagery of the years 1988, 1999, 2009 and 2019. Geo-spatial object-based supervised classification focusing on five major classes namely forest, agriculture, shrubland, settlement, and water body were applied. The overall classification accuracy was found 92 ~ 94% with a corresponding Kappa coefficient range of 0.90 ~ 0.93. The forest cover showed a major variation with the interval, decreasing from 234634 ha (76%) in 1988 to 168476 ha (54%) in 2019 with an annual loss of 2134.13 ha/year. Conversion results obtained from temporal overlay operation showed that forest patches converted to shrubland due to cutting down trees and forest burning and thereafter this shrubland cleared or re-burned for shifting cultivation which significantly increased agricultural land from 21787 ha (7%) in 1988 to 78704 ha (25%) in 2019 at the rate of 1836.03 ha/year. The area of other land use types including shrubland and settlement also increased. But the area of water body, the only source of drinking water to the indigenous community and irrigation water in the dry season, was found decreased with an average annual rate of 29.84 ha/year which indicate the drying of water body. The observed changing trend of LULC of the studied watershed could have a significant negative impact on the watershed environment as there would be more water shortage in the near future during the dry season and excess water in the rainy season. The findings of this study emphasizes important policy implications for sustainable watershed management in hilly regions of the tropics.
Stigma and Discrimination Against COVID-19 Positive Patients in Bangladesh: A Comparative Qualitative Study of Non-Vaccinated and Vaccinated Patients
Authors: Rashid Tamanna , Arefin Shamsul , Bhattacharjee Mowsume , Islam Md. Ashraful 
Published: January 2024

Abstract

Historically, pandemics have often led to the stigmatization and discrimination of those affected. Despite some studies detailing the stigmatized experiences of COVID-19 patients in Bangladesh, there is a noticeable absence of comparative research exploring the experiences of vaccinated and non-vaccinated COVID-19 survivors across different pandemic phases. This study aims to bridge this gap by comprehensively examining the stigma and discrimination encountered by both vaccinated and non-vaccinated COVID-19 survivors in Bangladesh. Employing a comparative qualitative case study approach, we conducted in-depth interviews with 22 COVID-19-positive individuals, comprising 13 interviews from the initial wave (between July 26 and August 12, 2020) and 9 interviews during the ongoing wave (between October 10, 2022, and April 10, 2023) of the pandemic in Bangladesh. Recruitment was facilitated through a targeted social media campaign, and a purposive sampling technique was applied. Interviews were predominantly held via telephone and online platforms. Subsequently, a thorough transcription of all interviews was carried out, followed by an in-depth thematic analysis. The findings highlight critical factors contributing to stigma and discrimination towards COVID-19-positive patients in Bangladesh, including the fear of death, infection, disease control measures (such as lockdowns, public announcements, and testing protocols), and individual experiences with COVID-19 testing. Notably, non-vaccinated participants conveyed a range of evolving experiences linked to COVID-19-related stigma and discrimination, differing from their vaccinated counterparts. The study also reveals that enhanced social support and normalized perceptions of COVID-19 played pivotal roles in mitigating stigma and discrimination. The insights gleaned from this study are poised to significantly inform healthcare policymakers in their efforts to devise effective pandemic control and management strategies. Moreover, by comprehending the socio-psychological nuances of stigmatization, this research offers novel perspectives for crafting policies that address emerging pandemic-related stigma in Bangladesh.
Health-related quality of life in in a large cohort of patients with cardiac implantable electronic devices – a registry based study
Authors: Gatti Paolo , Nymark Caroline , Gadler Fredrik 
Published: January 2024

Abstract
Aim
The association of cardiac implantable electronic devices (CIED), namely pacemaker (PM), implantable cardioverter-defibrillator (ICD) and cardiac resynchronization therapy with (CRT-D) or without defibrillator (CRT-P), on health-related quality of life (HRQoL) is lacking.
Methods and results
Data from the Swedish Pacemaker and ICD Registry together with the European Quality of Life-5 Dimension questionnaire (EQ-5D) administered before and after one year the CIED implant from January 2019 to February 2022 were used to analyze the HRQoL with the EQ-5D visual analogue scale (EQ-VAS) and the EQ-5D index.
Of 1,479 who completed the EQ-5D, 80% had a PM, 10% an ICD, 5% a CRT-P and 6% a CRT-D. The median age was 77 years with a prevalence of females ranging from 38% in the PM group to 17% in the ICD group. The EQ-VAS and the EQ-5D index significantly increased in patients with PM and CRT-P (EQ-VAS +2.8, standard deviations (SD) 23 and +5.8, SD 24.9; EQ-5D index +0.019, SD 0.114 and +0.051, SD 0.125) while only the EQ-5D index increased in patients with ICD (+0.002, SD 0.104 ). After adjusting for age, sex and HRQoL at baseline, the presence of defibrillator was associated with lower EQ-VAS (-3.4, 95% confidence intervals (CI) -6.7; -0.1 and -4.8, 95% CI -8.8;-0.7) and EQ-5D index (-0.018, 95% CI -0.035; -0.0003 and -0.025 95% CI 0.046;0.004) at follow-up compared to PM.
Conclusion
These findings, showing the HRQoL associated with CIED, are important to support physicians' and pacemaker nurses' care after device implantation by embracing the patients’ perspectives.
Integrating Periodontal Therapy and Biofilm Management into Breast Cancer Patients' Care: Periodontal, Hematological and salivary Parameters Assessment Before and During Chemotherapy
Authors: Villafuerte Kelly R. V. , Reis Francisco J Candido Dos , Carrara Hélio H. A. , Martinez Cristhiam , Palioto Daniela B. 
Published: January 2024

Abstract
Objective
To evaluate the impact of chemotherapy on periodontal conditions, hematology, and salivary flow in patients with breast cancer and gingivitis, after basic periodontal therapy (BPT).
Methods
They were divided into patients with breast cancer and gingivitis (BC/G = 20); and patients without cancer with gingivitis (G = 20). Clinical parameters [Plaque Index (PI), bleeding on probing (BOP), Probing Depth (PD), Clinical Attachment Level (CAL)], hematological parameters, and salivary flow were evaluated at baseline, 6, 12 and 24 weeks.
Results
After BPT, both groups exhibited a reduction in BOP and PI. However, group G showed a significantly (p < 0.05) greater reduction at weeks 12 and 24 compared to group BC/G. In the hematological parameters, the G group showed higher hemoglobin and red blood cell levels than the BC/G group at 6, 12, and 24 weeks (p < 0.05). The salivary flow rate was decreased at 6, 12, and 24 weeks in BC/G (< 0.3 ml/min), however, they did not show hyposalivation (< 0.1ml/min).
Conclusion
Chemotherapy significantly impacts periodontal and hematological conditions, as well as salivary flow. Both groups, showed improvement in periodontal conditions after BPT, with a reduction in BOP and PI. However, the group without cancer demonstrated significantly greater improvement compared to the group with cancer, indicating that chemotherapy affects inflammatory control. The application of BPT with strict biofilm control is necessary for breast cancer patients, contributing to an overall favorable treatment outcome.