bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of Hybrid Laparoscopic Hysterectomy Using the Hugo™ RAS System
Authors: KOMATSU HIROAKI , Sawada Mayumi , Iida Yuki , Wada Ikumi , Azuma Yukihiro , Kudoh Akiko , Sato Shinya , Harada Tasuku , Taniguchi Fuminori 
Published: January 2024

Abstract
Hybrid total laparoscopic hysterectomy combines conventional laparoscopic surgery and robot-assisted devices. During hybrid total laparoscopic hysterectomy, the camera and assistant forceps are operated by a robotic device, whereas the surgeon performs human procedures using a laparoscope. This enables surgery with a completely fixed field of view, thus significantly reducing errors in forceps grasping and needle misalignment. We created and implemented a new use for the Hugo™ robot-assisted surgery system, which consists of independent arms. This new technique involving new equipment can improve surgeon training and performance. We will continue to develop new techniques that can further improve surgical performance.
Point-of-care ultrasound (POCUS) pediatric resident training course: a cross-sectional survey
Authors: Bianco Manuela Lo , Presti Santiago , Finocchiaro Maria Carla , Trobia Gian Luca , Sciacca Tiziana Virginia , Cucuzza Maria Elena , Caudullo Elia , Calcara Giacomo , Ruggieri Martino , Stefano Vita Antonella Di 
Published: January 2024

Abstract
Background:Point-of-care ultrasound (POCUS) is becoming increasingly crucial in the Pediatric Emergency Department for objective patient examination. However, despite its growing interest and wide-ranging applications, POCUS remains relatively unexplored in general pediatric training and education. Many physicians still find it challenging to comprehend and implement.
Methods:a theoretical-practical POCUS course for pediatric residents was conducted at the University of Catania, Italy. The course's effectiveness and practical impact on residents was assessed through a pre-post training survey. The first part of the questionnaire focused on the self-perceived time needed to learn how to recognize the following conditions using POCUS: i) Pleural effusion ii) Lung consolidation iii) Pneumothorax (PNX) iv) Cardiac contractility v) Pericardial effusion vi) Perisplenic effusion vii) Morison’s pouch effusion viii) Douglas' pouch effusion ix) Filling and collapsibility of the inferior vena cava. In the second part, we compared the potential role of POCUS in i) Reducing the use of ionizing radiation in children ii) Increasing the sense of security in diagnosis and treatment decisions making and iii) Increasing the residents' confidence level with POCUS after the course on a 1-to-10 rating scale.
Results:seventy-two residents participated in the study. The statistical analysis showed significant pre-post differences in almost all the items considered, except for "cardiac contractility" and "PNX".Furthermore, the perceived potential role of POCUS in reducing ionizing radiation usage and the sense of security in diagnosis and treatment decisions showed statistically significant differences (p<0.05) before and after the course. Data analysis also revealed a consistently high confidence level with POCUS after the course.
Conclusions:the results highlight the importance of including a POCUS track course in pediatric post-graduate programs due to its simplicity, rapid learning time, and clinical usefulness. Based on these findings, it would be recommended to increase the teaching hours dedicated to the recognition of pneumothorax and cardiology POCUS examination. Emphasizing POCUS training in pediatric education can enhance patient care and diagnostic accuracy while minimizing radiation exposure.
Clinical significance of increased levels of endogenous Muse cells in the myocardium of patients with fulminant myocarditis
Authors: Toyoda Shigeru , Sakuma Masashi , Ishida Kazuyuki , Kushida Yoshihiro , Soma Ryoichi , Takayama Hidehito , Akimoto Kazumi , Dezawa Mari , Inoue Teruo 
Published: January 2024

Abstract
Background: The significance of Muse cells, stress-tolerant endogenous pluripotent-like reparative stem cells involved in tissue repair, in acute myocarditis has not been evaluated.
Methods: Muse (SSEA-3+) cells/area were counted in biopsied myocardial tissue samples from 17 patients with fulminant myocarditis (51±19 years) and 6 with non-inflammatory myocardial disease (69±5 years, control). Patients were segregated according to clinically and histopathologically relevant items, and further segregated by median values of echocardiographic parameters and biomarkers in acute and recovery phases for stratification into Yes/No groups. Categorical variables with binary values were assigned Yes or No and continuous variables were stratified based on the median value (values indicating more critical status or poorer recovery assigned to the Yes group).
Results: Compared with controls, patients with fulminant myocarditis had significantly more Muse cells (P=0.00042). Patients with mechanical circulatory support (P=0.006), myocardial degeneration (P=0.023), and an acute-phase creatine kinase-myocardial band (CK-MB) level >71 U/L, indicating more critical status, had significantly more Muse cells (P=0.008). Patients with a higher acute/recovery phase ratio (indicator of recovery) of CK-MB <15.2 and cardiac troponin I <207.9, indicating poorer recovery, had significantly fewer Muse cells than patients that did not (better recovery; P=0.008 and =0.033, respectively). Patients with a C-reactive protein (CRP) level >0.10 mg/dL during recovery had a lower Muse cell number than patients with CRP <0.10 (P=0.046).
Conclusions: Muse cell number in acute phase myocardium biopsy specimens correlated with the severity of clinical features in the acute phase and the recovery from myocardial damage in the chronic phase. Myocardial biopsy combined with Muse cell detection by anti-SSEA-3 staining might be useful for evaluating disease severity and predicting treatment responsiveness in fulminant myocarditis.
A Burden of Rare Copy Number Variants in
Obsessive-Compulsive Disorder
Authors: Halvorsen Matthew , de Schipper Elles , Boberg Julia , Strom Nora , Hagen Kristen , Lindblad-Toh Kerstin , Karlsson Elinor , Pedersen Nancy , Bulik Cynthia , Fundín Bengt , Landén Mikael , Kvale Gerd , Hansen Bjarne , Haavik Jan , Mattheisen Manuel , Rück Christian , Mataix-Cols David , Crowley James 
Published: January 2024

Abstract
Current genetic research on obsessive-compulsive disorder (OCD) supports contributions to risk specifically from common single nucleotide variants (SNVs), along with rare coding SNVs and small insertion-deletions (indels). The contribution to OCD risk from large, rare copy number variants (CNVs), however, has not been formally assessed at a similar scale. Here we describe an analysis of rare CNVs called from genotype array data in 2,248 deeply phenotyped OCD cases and 3,608 unaffected controls from Sweden and Norway. We found that in general cases carry an elevated burden of large (>30kb, at least 15 probes) CNVs (OR=1.12, P=1.77x10-3). The excess rate of these CNVs in cases versus controls was around 0.07 (95% CI 0.02-0.11, P=2.58x10-3). This signal was largely driven by CNVs overlapping protein-coding regions (OR=1.19, P=3.08x10-4), particularly deletions impacting loss-of-function intolerant genes (pLI>0.995, OR=4.12, P=2.54x10-5). We did not identify any specific locus where CNV burden was associated with OCD case status at genome-wide significance, but we noted non-random recurrence of CNV deletions in cases (permutation P = 2.60x10-3). In cases where sufficient clinical data were available (n=1612) we found that carriers of neurodevelopmental duplications were more likely to have comorbid autism (P<0.001), and that carriers of deletions overlapping neurodevelopmental genes had lower treatment response (P=0.02). The results demonstrate a contribution of large, rare CNVs to OCD risk, and suggest that studies of rare coding variation in OCD would have increased power to identify risk genes if this class of variation were incorporated into formal tests.
Unraveling Organoboron/Nickel Ion-Pair Interactions via Benchtop-Stable Carbyl Iminopyridyl NiII Complexes for Olefin Polymerization
Authors: Harth Eva , Rauf Hasaan , Liu Yu-Sheng , Solanki Surya Pratap , Deydier Eric , Poli Rinaldo , Grabow Lars 
Published: January 2024

Abstract
While current research on Ni-catalyzed olefin polymerization predominantly focuses on ligand design, ion-pair interactions remain largely unexplored. We report the development of air-stable carbyl iminopyridyl NiII precatalysts to enable an investigation of inner- and outersphere Ni ion-pairs. The use of innersphere organoboron counterions allows the Ni complexes to access higher molecular weight homo/co-polymers and regulate the density and distribution of polyethylene branches. Moreover, implementing a phenyl group on the tether carbon functioned as a rotational barrier, producing higher molecular weight polymers compared to methylsubstituted analogs. A controlled incorporation of shortchain branches was achieved under high ethylene pressure, circumventing the need for elaborate ligand design, low monomer pressures, and the copolymerization with α-olefins. DFT calculations further elucidated the ion-pair interactions and controlled chain-walking mechanism. Here, we provide a new perspective to manipulate the iminopyridyl NiII system leveraging both ion-pair interactions and ligand design to govern polyolefin molecular weights and microstructures.
Retinal degeneration protein 3 mutants are associated with cell-cycle arrest and apoptosis
Authors: Bräuer Anja , Hausmann Jens , Zimmermann Benjamin , Chen Yaoyu , Helgers Simeon , Dömer Patrick , Woitzik Johannes , Raap Ulrike , Gray Natalie , Büttner Andreas , Koch Karl-Wilhelm 
Published: January 2024

Abstract
Retinal degeneration protein 3 (RD3) plays a crucial role in controlling guanylate cyclase activity in photoreceptor rod and cone cells, and mediates trafficking processes within photoreceptor cells. Loss of RD3 function correlates with severe forms of retinal dystrophy and the development of aggressive neuroblastoma cancer. In the present study, we analysed RD3 expression by applying a data-mining approach using public databases. In addition, we performed an RD3 transcript analysis on specimens of glioma tissues from patients. We found that RD3 is downregulated in glioblastoma compared to non-tumor tissues. Low expression of RD3 in gliomas was also associated with a poor prognosis of survival rate. Applying a multi-cohort receiver operating characteristic test, we verified significant changes in RD3 expression in glioblastomas, indicating a potential use of RD3 in glioblastoma diagnosis. The apparent influence of RD3 on cell viability or on cell cycle progression we examined by overexpressing RD3 in a cell culture system. Wild-type RD3 significantly decreased cell viability, which subsequently led to cell-cycle arrest at the G2/M phase and induced cell apoptosis. Conversely, single-point mutations in RD3 at the exposed protein surface involved in RD3 target interaction diminished the impact of RD3 wild type, thereby showing its specificity. Therefore, expression of wild type RD3 might prevent tumor progression by facilitating the death of cancer cells or arresting the cell cycle in a developing tumor. Our findings further suggest that RD3 is a potential prognostic factor in glioblastoma.
Clinical observation of the formation of fat particles and fat liquefaction in a CIED pocket: A case report
Authors: Guan Aili , He Tao , Huang Xingang , Xu Wenliang , Shao Yibing , Chen Xueying 
Published: January 2024

Abstract
Background The number of implanted cardiovascular implantable electronic devices (CIEDs) has increased significantly in recent years, which has led to an upsurge in CIED complications, such as infection and lead malfunction requiring CIED extraction. Few cases of fat liquefaction and fat particles in the CIED pocket have been reported. And no pathological diagnosis was found.Case presentation A 71-year-old Chinese woman presented with Pacemaker failure and asthma. Fat liquefaction and fat particles in the pacemaker pocket were found during the replacement procedure. The patient had no symptoms and no signs of infection or other discomfort of the heart and pacemaker pocket. Thick yellow liquid was seen to flow out of the pacemaker pocket when the pocket was opened, and many fat particles were found adhering to the wall of the pacemaker pocket during the pacemaker replacement procedure. Fat and fibrillar connective tissue with a few inflammatory cells, local tissue degeneration and necrosis were shown on immunohistochemical staining and no bacterial growth including anaerobic bacteria was observed. No redness, swelling, fever, or wound pain were observed during the nine-month follow-up period.Conclusion The presence of fat liquefaction and fat particles in a CIED pocket may be related to the necrosis of fat cells.
An adaptation of the goggles experiment for dogs: Evidence that dogs are unable to use their own experience to infer a human’s visual perspective
Authors: West-Brownbill Amy , Proops Leanne , Herrmann Esther , Kaminski Juliane 
Published: January 2024

Abstract
Dogs have strong bonds with humans but the extent to which they can take our perspective into account is still in question. This study adapts the goggles experiment methodology to investigate if domestic dogs engage in level 2 perspective taking. The dogs not only have to understand when the human individual’s line of sight is blocked (level 1 perspective taking), but have to infer the other individual’s visual experience based on their own previous experience. A competitive context of food stealing is used. Dogs (N = 24) first gained experience with two compartments, learning that food was accessible from one end while the other end had either a transparent or opaque lid. When stealing the food, dogs were visible to the human only through the transparent lid. In the test, dogs experienced two conditions: where both compartments were baited and a human either remained in the room (social condition) or left the room (non-social condition). Three factors were observed: (i) whether the dog took the food, (ii) the compartment the dog stole from, and (iii) latency to steal. The findings revealed that dogs didn't exhibit a preference for stealing from the concealed side, demonstrating that dogs don't consider the human's perspective when selecting which food to steal. However, in the social condition more dogs refrained from stealing, demonstrating sensitivity to the scenario’s competitive nature. It’s possible that the setting was overall too challenging and future research should look for alternative procedures to address the question of dogs’ understanding of a human’s perspective.
High Energy X-ray Source Characterization at 0.450, 3, 6, 9, and 15 MVp
Authors: Hellmann David , Liesenfelt Michael , Hayward Jason P. 
Published: January 2024

Abstract

As the availability and importance of high energy X-ray sources grows, accurate source characterizations provide critical information for field flatness corrections, beam hardening corrections, detector response corrections, and radiation shielding assessments. This study uses MCNP6.2 to create accurate high definition angular and energy dependent X-ray source definitions for the most common high energy industrial X-ray sources at 450 kVp, 3 MVp, 6 MVp, 9 MVp, and 15 MVp.
Integrating post-hospital care by digital counseling tools: A non-randomized proof-of-concept study
Authors: Röglin Julia , Nitschke Johanna , Kleemann Tobias , Ortmann Steffen 
Published: January 2024

Abstract
Background
The duration of stays in hospitals have decreased by almost 50% to an average of 7.2 days in 2021 compared to 1992 whilst reliance on internet-based health information has increased. This trend raises concerns about potential misinterpretations and the need for enhanced post-hospital support.
Methods
This proof-of-concept study established a chat-based recovery counseling service providing nursing expertise and digital counseling options to patients within 7 days after discharge from hospital. Therefore, real nursing professionals where available to respond to patient queries and questions. A chatbot assisted the counselor by suggesting potential responses based on the patient's questions. This chatbot was trained using the expertise of nursing professionals. The study aimed to assess patients' acceptance, nursing professionals' commitment, and patients' willingness to contribute chat interactions and chat content for further research and tool developments. Surveys and interviews were conducted with recovery counselors to explore their attitudes towards digitalization, self-assessed digital competencies, and potential changes to the service structure.
Results
Within one year, 247 patients across five stations (surgery, oncology, and orthopedics) were introduced to the digital recovery counseling service. Several patients declined to participate in the study, with the main reason for refusal voluntarily given by patients being the lack of a PC in the household (68.86%). Patients in the 51–70 age group showed the highest positive responses. Out of the consenting patients, all but one agreed to donate their chat history, and 21 of the participants registered on the platform. Neurosurgery patients exhibited the highest interest, while oncology patients had limited interest due to pre-existing information. Recovery counselors reported varying degrees of improved digital competencies. The chatbot presented challenges for uniform training across specialties due to its limited dataset, emphasizing the need for a broader question set for comprehensive training.
Conclusions
The study shows patients acceptance for digital counselling via chat, emphasizing also nursing staff's readiness for digital expansion. Integrating digital training is vital to overcome initial doubts. Patients willingly donate data with clear information, showcasing the chatbot's potential as a nursing expert system. Expanding chat-based nurse counselling may enhance post-inpatient advice, necessitating future considerations for broader deployment.
Agreement of point of care ultrasound and final clinical diagnosis in patients with acute decompensated heart failure, acute coronary syndrome, and shock: POCUS not missing the target
Authors: Ramos José Atilio Núñez , Misol Dagoberto Duarte , Petro María Andrea Burgos , Pérez Keren Jemima Sarmiento , Echeverry Vanesa Paola Gutiérrez , Velasco-Malagón Sergio 
Published: January 2024

Abstract
Introduction: Point-of-Care ultrasound (POCUS) is an important tool for clinical diagnosis and decision-making in critical and non-critical scenarios. Dyspnea, chest pain and shock are conditions valuable to evaluate with ultrasound considering diagnostic accuracy and clinical impact already proven. There is scarce evidence in diagnosis agreement using ultrasound as an extension of physical examination. We aimed to evaluate ED patients in whom POCUS was performed, to analyze agreement between clinical initial diagnosis using ultrasound images and final diagnosis. Furthermore, we analyze failed diagnosis, inconclusive POCUS exams and discuss details.
Methods: A cross-sectional analytical study was conducted in adults who visited the emergency department with any of these three chief complaints: dyspnea, chest pain, shock. All were evaluated with ultrasound at admission. Agreement between initial diagnosis using POCUS and final definite diagnosis was calculated. Failed diagnosis and inconclusive exams were analyzed.
Results: A total of 209 patients were analyzed. Populations mostly males, mean age 64yo, hypertensive. Patients with dyspnea, agreement in heart failure diagnosis was 0.98; agreement in chest pain with non-ST acute coronary syndrome was 0.96; agreement in type of shock was 0.90. Twelve 12 (5%) patients had an inconclusive POCUS exam, and 16 (7%) patients had a failed diagnosis.
Conclusion: The use POCUS in the emergency department shows almost perfect agreement when compared with the final diagnosis in individuals experiencing acutely decompensated heart failure, acute myocardial infarction, and shock. Prospective studies are needed to evaluate the impact of this tool on mortality and prognosis when there are diagnostic errors.
Immunological sub-phenotypes and response to Convalescent Plasma in COVID-19 induced ARDS: a secondary analysis of the CONFIDENT trial.
Authors: Misset Benoit , Diep Anh Nguyet , Bertrand Axelle , Piagnerelli Michael , Hoste Eric , Michaux Isabelle , De Waele Elisabeth , Dumoulin Alexander , Jorens Philippe G , Hauwaert Emmanuel van der , Vallot Frédéric , Swinnen Walter , De Schryver Nicolas , de Mey Nathalie , Layios Nathalie , Mesland Jean-Baptiste , Robinet Sébastien , Cavalier Etienne , Donneau Anne-Françoise , Moutschen Michel , Laterre Pierre-François 
Published: January 2024

Abstract
Background
Convalescent Plasma (CP) reduced the mortality in COVID-19 induced ARDS (C-ARDS) patients treated in the CONFIDENT trial. As patients are immunologically heterogeneous, we hypothesized that clusters may differ in their treatment responses to CP.
Methods
We measured 20 cytokines, chemokines and cell adhesion markers using a multiplex technique at the time of inclusion in the CONFIDENT trial in patients of centers having accepted to participate in this secondary study. We performed descriptive statistics, unsupervised hierarchical cluster analysis, and examined the association between the clusters and CP effect on day-28 mortality.
Results
Of the 475 patients included in CONFIDENT, 391 (82%) were sampled, and 196/391 (50.1%) had been assigned to CP. We identified four sub-phenotypes representing 89 (22.8%), 178 (45.5%), 38 (9.7%), and 86 (22.0%) patients. The most contributing biomarkers in the principal component analysis were IL-1β, IL-12p70, IL-6, IFN-α, IL-17A, IFN-γ, IL-13, TFN-α, total IgG, and CXCL10. Sub-phenotype-1 displayed a lower immune response, sub-phenotype-2 a higher adaptive response, subphenotype-3 the highest innate antiviral, pro and anti-inflammatory response, and adhesion molecule activation, and sub-phenotype-4 a higher pro and anti-inflammatory response, migration protein and adhesion molecule activation. Sub-phenotype-2 and sub-phenotype-4 had higher severity at the time of inclusion. The effect of CP treatment on mortality appeared higher than standard care in each sub-phenotype, without heterogeneity between sub-phenotypes (p = 0.97).
Conclusion
In patients with C-ARDS, we identified 4 sub-phenotypes based on their immune response. These sub-phenotypes were associated with different clinical profiles. The response to CP was similar across the 4 sub-phenotypes.