bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Lavender on Mood Disorders Associated with the Use of Combined Oral Contraceptives (COCs): A Triple-Blinded Randomized Controlled Trial
Authors: Naghdi Mina , Farshbaf-Khalili Azizeh , Nahaee Jila , Hakimi Parvin , Shahnazi Mahnaz 
Published: October 2023

Abstract
Background: Combined oral contraceptives (COCs) are reliable medications used as a contraception method. One of the most common reasons for discontinuing COCs is their association with negative mood disorders. Studies have confirmed the anti-anxiety and mood-stabilizing effects of lavender.
This study was conducted to investigate the effects of lavender essential oil (LEO) on mood disorders associated with the use of COCs.
Methods: This triple-blinded clinical trial was conducted on 60 married women (aged 15-49 years old) who were consumers of COCs, referring to 26 health centers in Tabriz, Iran. The participants were randomly assigned to either the intervention (consuming one gelatin capsule containing 80 mg LEO daily) or control (consuming one placebo capsule daily) group. The intervention continued for 56 days. Scores for Positive and negative were determined using the Positive and Negative Affect Schedule (PANAS) questionnaire, and stress, depression, anxiety were measured using the DASS-21 questionnaire on day’s 28th and 56th post-intervention. Data analysis was conducted using the t-test and ANOVA with the RM model and a p-value of < 0.05 was considered significant for all analyses.
Results: A statistically significant difference was observed in mood disorders, stress, and depression between women receiving LEO or placebo. The consumption of LEO increased the positive mood on day 28 [MD (95% CI): 4.5 (2.1 to 7.0), p=0.001] and day 56 [5.9 (3.4 to 8.3), p<0.001] while decreased the negative mood on day 28 [MD (95% CI): -3.5 (-5.3 to -1.3), p<0.001] and day 56 [-4.3 (-6.3 to -2.2), p<0.001] , stress on day 28 [MD (95% CI): -4.9 (-7.1 to -2.8), p=0.001] and day 56 [-5.3 (-7.6 to -3.1), p<0. 001], and depression on day 28 [MD (95% CI): -3.0 (-4.9 to 1.1), p=0.003] and day 56 [-3.1 (-5.0 to 1.2), p=0.002]. There was no statistically significant difference between the two groups in terms of anxiety.
Conclusions: The consumption of LEO with COCs improved mood disorders and reduced stress and depression. It is expected that appropriate counseling and education should be offered to women using COCs regarding the possibility of mood changes, providing appropriate solutions, including the simultaneous use of LEO.
The registration number: IRCT20110606006709N24.
The registration date: 20/04/2022
Temporal overlapping effect in pump-probe technique on the Rhodamine 6G with addition copper nanoparticle
Authors: khalid Saad 
Published: October 2023

Abstract
This study took place utilizing the laser "Pump-Probe" probe technique to investigate the properties of a rhodamine 6G laser dye. 532 nm wavelength laser was employed for excitation of the dye "pumping", while another laser with a wavelength of 657 nm was utilized for sensitization "probe". A copper nanomaterial with an average size of 43 nm has been added to the system. The green laser pulse width was modulated at various percentages, including 5%, 10%, 20%, 30%, 40%, and 50% of the original pulse width. The pulse utilized for the pumping process was divided into two components, one of the tow pulse was directed towards the dye, while optical path of the other component was altered by employing multiple mirrors, the modification in the optical path was implemented in order to establish an accurate delay time between the two pulses and control their overlap by extending the duration of the laser pulse. The transmittance laser intensity was determined both before and after the addition of the nanomaterial. Additionally, (FWHM) of the output laser pulse was computed. The wavelength of the output laser pulse was also determined through calculation. The study findings demonstrated a discernible impact resulting from the alteration of laser pulse width as well as the influence of the nanomaterial on the intensity and optical characteristics of the dye. This study is a component of a series of research endeavors focused on comprehending the interplay between nanomaterials and laser dyes as well as the resultant impact of these interactions on the characteristics of laser dyes.
Risk of stroke in patients with psoriatic disease: a systematic review and meta-analysis
Authors: Li Zhengjun , Chen Fuqiang , Li Xueqing , Li Chengguo , Chen Zhixin , Freel Colman I. , Mu Zhen , Zhao Yingjie 
Published: October 2023

Abstract
Stroke is a neurologic disease caused by sudden disturbance of cerebral blood circulation. Studies have shown that patients with psoriatic disease (PsD) (psoriasis and psoriatic arthritis (PsA)) may have an increased risk of stroke, but the results are inconsistent. The aim of this study was to provide an updated meta-analysis about the risk of stroke in PsD. Electronic databases was searched to identify relevant studies. Random-effects model was used to analyze the risk of stroke in patients with PsD. Subgroup analyses were conducted to identify sources of heterogeneity between studies and further explore risk relationships. This meta-analysis found an increased risk of stroke in patients with PsD, with the pooled relative risk (RR) of 1.23 (95% confidence interval (CI): 1.20-1.27). In subgroup analysis stratified by severity of psoriasis, the risk of stroke was higher in severe (RR = 1.42, 95% CI: 1.22-1.65) than in mild psoriasis (RR = 1.12, 95% CI: 1.03-1.21). In the subgroup analysis of stroke type, PsD patients had a greater risk of ischemic (RR = 1.49, 95% CI: 1.26-1.76) than hemorrhagic stroke (RR = 1.30, 95% CI: 0.80-2.11). The RRs of stroke in PsD patients from Asia, Europe, and the United States were 1.26 (95% CI: 1.08-1.46), 1.19 (95% CI: 1.08-1.31), and 1.28 (95% CI: 0.75-2.17), respectively. Patients with PsD have an increased risk of stroke. Severe psoriasis patients may have a higher risk of stroke than mild.
Trial registration: This study was registered at international prospective register of systematic reviews (PROSPERO), registration number: CRD42022365700.
Synthetic organelles for energy conservation and delivery of building blocks for lipid biosynthesis
Authors: Poolman Bert , Bailoni Eleonora , Patiño-Ruiz Miyer , Stan Andreea , Schuurman-Wolters Gea , Exterkate Marten , Driessen Arnold 
Published: October 2023

Abstract
ATP is a universal energy currency that is essential for life. ATP generation based on the degradation of L-arginine can be used for the bottom-up construction of synthetic cells. In an earlier design of this pathway, a relatively slow L-arginine/L-ornithine antiporter limited ATP production. After testing homologous proteins, we now implement an antiporter (ArcD from Lactobacillus sakei) with improved transport kinetics, leading to faster ATP recycling in organelle-like vesicles. Furthermore, we exploit the L-arginine-dependent ATP formation for the synthesis and export of glycerol-3-phosphate with complete recycling of waste products, ensuring sustainable production of building blocks. The exported glycerol-3-phosphate from the feeding vesicles serves as a precursor for the biosynthesis of phospholipids in utilizer vesicles, which provides the basis for the expansion of the cell membrane. We thus develop an out-of-equilibrium metabolic network for ATP generation and couple metabolic energy production to lipid synthesis in an organelle-like system, inspired by compartmentalization in eukaryotic cells.
CK2α-mediated phosphorylation of GRP94 facilitates the metastatic cascade in triple-negative breast cancer
Authors: Hong Suntaek , Kim Hye-Youn , Kim Young-Mi 
Published: October 2023

Abstract

Distant metastasis is a significant hallmark affecting to the high death rate of patients with triple-negative breast cancer (TNBC). Thus, it is crucial to identify and develop new therapeutic strategies to hinder cancer metastasis. While emerging studies have hinted a pivotal role of glucose-regulated protein 94 (GRP94) in tumorigenesis, the exact biological functions and molecular mechanisms of GRP94 in modulating cancer metastasis remain to be elucidated. Our study demonstrated an increased expression of GRP94 in TNBC correlated with metastatic progression and unfavorable prognosis in patients. Functionally, we identified that GRP94 depletion significantly diminished TNBC tumorigenesis and subsequent lung metastasis. In contrast, GRP94 overexpression exacerbated the invasiveness, migration, and lung metastasis of non-TNBC cells. Mechanistically, we found that casein kinase 2 alpha (CK2α) active in advanced breast cancer phosphorylated GRP94 at a conserved serine 306 (S306) residue. This phosphorylation increased the stability of GRP94 and enhanced its interaction with LRP6, leading to activation of canonical Wnt signaling. From a therapeutic standpoint, we found that benzamidine, a novel CK2α inhibitor, effectively suppressed GRP94 phosphorylation, LRP6 stabilization, and metastasis of TNBC. Our results point to the critical role of CK2α-mediated GRP94 phosphorylation in TNBC metastasis through activation of Wnt signaling, highlighting GRP94 as a therapeutic target to impede TNBC metastasis.
Clarification attempt of the mechanism of late recurrence by micro- and macro-analyses in estrogen receptor-positive breast cancer
Authors: Kitano Sae , Tsunashima Ryo , Kato Chikage , Watanabe Akira , Sota Yoshiaki , Matsumoto Saya , Morita Midori , Sakaguchi Koichi , Naoi Yasuto 
Published: October 2023

Abstract
Purpose
The mechanism of late recurrence (LR) of estrogen receptor (ER)-positive breast cancer remains unclear. As prediction models for LR of ER-positive breast cancer, 42-gene classifier (42GC), which analyzes “micro-factors (gene expression patterns)” and the Clinical Treatment Score post-5 years (CTS5), which analyzes “macro-factors (clinicopathological factors)”, were developed; however, improving the accuracy of these models is desirable. We aimed to clarify the mechanism and develop a new prediction model by combining 42GC and CTS5.
Methods
We selected 2,454 patients with ER-positive breast cancer from public microarray databases. We performed recurrence prognostic analysis using 42GC and CTS5.
Results
In “the basic research” for recurrent patients (n = 347), the 42GC LR and CTS5 low-risk groups tended to have LR. In “the clinical research” for recurrence-free patients 5 years after surgery (n = 671), the 42GC LR and CTS5 high-risk group had a significantly higher LR rate after 5 years (16.9%) than the 42GC non-LR and CTS5 low-risk group (5.41%) (p = 0.037).
Conclusion
In “the basic research,” we found that both micro-and macro-factors were associated with the mechanisms of early recurrence and LR. Meanwhile, in “the clinical research,” we found that the mechanistic tendency toward LR (the CTS5 low-risk group) differed from the high rate of LR (the CTS5 high-risk group). Therefore, differentiating between the biological mechanisms elucidated in “the basic research” and the decision-making process concerning extended hormonal therapy in “the clinical research” is necessary. These findings propose the development of a novel prediction model for LR.
Proteus vulgaris strain Ld01-originated mandelonitrile defends Leptinotarsa decemlineata larvae and adults against predation
Authors: Li Guo-Qing , Kang Wei-Nan , Zhang Xiao-Qing , Jin Lin , Fu Kai-Yun , Guo Wen-Chao 
Published: October 2023

Abstract

Leptinotarsa decemlineata larvae and adults have evolved to apply aposematic signals to display their toxicity to natural enemies. Nevertheless, the chemical components of the volatile repellant remain to be identified. Here we show that the larvae and adults actively release hypertoxic hydrogen cyanide (HCN) from the degradation of nitriles, in contrast to the pupae. A bacterial symbiont Proteus vulgaris Ld01 is responsible for the production of a cyanoglucoside, amygdalin. The bacterium mainly lives in beetle guts and proliferates rapidly during ecdysis. Removal of larval bacteria significantly reduces HCN emission in the larvae and adults. Re-infection with the symbiont in or addition of mandelonitrile to the bacteria-deprived beetles restores the emission. Deprivation of bacteria decreases the defense of the larvae and adults against chick predation, which can be mitigated by re-colonization of P. vulgaris Ld01 or introduction of mandelonitrile. Together, these findings highlight the roles of intestinal P. vulgaris Ld01 in the production of an aromatic cyanoglucoside amygdalin in its host L. decemlineata. Degradation of amygdalin liberates an aposematic compound mandelonitrile to the hemolymph. Mandelonitrile and its metabolites are accumulated near the cuticle, where they are converted to HCN to defend the beetles against predation.
Gut microbiota and male fertility: a two-sample Mendelian randomization study
Authors: Kang YiFan , Wang ZhiHong 
Published: October 2023

Abstract
Background
Previous studies have reported that alterations in gut microbiota composition are associated with male fertility. However, it is unclear and difficult to establish whether these associations reflect a causal relationship.
Objective
To reveal the causal association between gut microbiota and male fertility, we conducted a two-sample Mendelian randomization (MR) analysis.
Materials and Methods
We assessed genome-wide association study (GWAS) summary statistics for gut microbiota and male fertility to perform MR analysis. Independent single nucleotide polymorphisms closely associated with 211 gut bacterial taxa (N = 122110) were identified as instrumental variables. The summary statistic data for male infertility (N = 733,479), abnormal spermatoz (N = 209,921) and erectile dysfunction (N = 223,805) were obtained from the latest release from the FinnGen consortium as the outcome of interest. Two-sample MR was performed to evaluate the causal effect of gut microbiota on male fertility, including inverse-variance-weighted (IVW) method, weighted median method, MR-Egger, mode-based estimation and MR-PRESSO. A series of sensitivity analyses was performed to validate the robustness of the results. The robustness of the estimation was tested by a series of sensitivity analyses including Cochran’s Q test, MR-Egger intercept analysis, leave-one-out analysis and funnel plot were used to assess the causal association.
Results
Combining the results from the discovery and replication stages, we identified three causal bacterial genus. Ruminiclostridm6 (OR = 0.537, 95%CI = 0.292-0.987, P = 0.045, PFDR = 0.234) was found to be closely associated with male infertility, and the decrease in its quantity increased the risk of male infertility. Decreased Prevotella9 (OR = 0.670, 95%CI = 0.452-0.992, P = 0.046, PFDR = 0.175) was found to be closely related to abnormal sperm. Lachnospiraceae NC2004 group (OR = 1.173, 95%CI = 1.008-1.366, P = 0.078, PFDR = 0.530) was found to be closely related to male erectile dysfunction, and there was a positive correlation between them. No heterogeneity and pleiotropy were detected.
Conclusion
This study implied a causal relationship between the Ruminiclostridm6 genus, Prevotella9 genus, Lachnospiraceae NC2004 group genus and male fertility, thus providing novel insights into the gut microbiota-mediated development mechanism of ADs. Nevertheless, future studies are warranted to dissect the underlying mechanisms of specific bacterial taxa’s role in the pathophysiology of male fertility.
Effects of urban forms on the Thermal-Acoustic-Air comfort in high-density neighborhoods: A case study of Shuangjing subdistrict, Beijing
Authors: Zhou Dawei , Wu Bomeng 
Published: October 2023

Abstract
Improving environmental comfort can help promote residents’ health and well-being in urban areas. This article takes Shuangjing subdistrict in Beijing as an example to explore the complicated effects of urban forms on the thermal-acoustic-air comfort in high-density neighborhoods aiming to provide urban regeneration strategy supports for improving the environmental comfort of residents. The results show that the eastern region exhibits better thermal-air comfort, while the southwest region demonstrates greater acoustic-air comfort. The central region experiences the poorest acoustic-thermal comfort but boasts the highest air comfort. In addition, all three types of environmental comfort are influenced by sky view factor (SVF), spatial enclosure, research unit area, and building height, with distinct underlying mechanisms. The research findings demonstrate that regarding urban forms air comfort and acoustic comfort share similar requirements while thermal comfort and acoustic comfort exhibit opposing demands. Urban designers need to carefully balance the spatial requirements for these three comfort types. Zoning management and design strategies emerge as feasible approaches.
Identification of Lactic Acid Bacteria in Spontaneous Fermented Cassava Food Product
Authors: Wahyuni S. , Dewi Novi Dian Puspita , Pato U. , Susilowati P. E. , Khaeruni Andi , Fatahu Fatahu 
Published: October 2023

Abstract
Lactic Acid Bacteria (LAB) such as Lactobaccilus is very important in digestive system health. One source of lactic Acid Bacteria is from cassava fermented foods. this research aimed to identify the genetic diversity of amylase-producing lactic acid bacteria isolated from fermented cassava. Fermentation was conducted by immersion of cassava in seawater for 6 hours and incubated for 3 days. analysis of morphology shows that the bacterial colonies has shape of bacilli and cocci cells, which are Gram-positive type. PCR analysis reveals that the bacterial DNA sequences Cladogram follows a Klad sister group Lactobacillus plantarum strain CQ2017ZC MH727586.1 with a bootstrap value of 77, Pediococcus pentosaceus strain 1931 MT597748.1 and P. Pediococcus pentosaceus strain 5583 MT510326.1, with a bootstrap value of 100. The analysis of genetic diversity showed that lactic acid bacteria from cassava fermentation had genetic similarities with lactic acid bacteria from milk. The results of chemical analysis showed that the isolation of lactic acid bacteria (UM 48.1, UM 48.2 and UM 24.2) has amylase activity of enzyme 446 mU/mL, 499 mU/mL and 1053 mU/mL respectively. These two Pediococcus pentosaceus have application potential in the chemical, pharmaceutical, food, and healthcare sectors. Furthermore, this study may provide an alternative to making milk-based health food and new fermented products using Lactic Acid Bacteria from cassava fermentation.
Endogenous cell membrane interactome mapping for GLP-1 receptor via ligand-based proximity labeling
Authors: Shui Wenqing , Dang Ting , Yu Jie , Cao Zhihe , Zhang Bingjie , Li Shanshan , Xin Ye , Yang Lingyun , Lou Ronghui , Zhuang Min 
Published: October 2023

Abstract

GLP-1 receptor, one of the most successful targets for therapeutic drug development against type 2 diabetes and obesity, is known to engage multiple intracellular proteins to initiate different signaling pathways. However, due to technical challenges, it remains much less explored how the receptor interaction with proteins on the cell membrane mediates its signaling activity and physiological effects. Here, we present a ligand-based proximity labeling approach to be integrated with quantitative proteomics so as to interrogate the native cell membrane interactome for the GLP-1 receptor upon agonist simulation. With this approach, our study identified a number of unreported cell membrane interactors for the endogenous receptor in both a pancreatic β cell line and a neuronal cell line, which revealed interaction landscapes distinct from those previously mapped in less physiological cellular systems. We further demonstrate this strategy can be exploited to uncover new regulators of GLP-1 receptor-mediated signaling and insulinotropic response in β cells. Additionally, we obtain a time-resolved cell membrane interactome map for the receptor in β cells. Therefore, our study provides a new approach that is generalizable to map endogenous cell membrane interactomes for GPCRs so as to decipher the molecular basis of their cell-type-specific functional regulation.
Reservoir-computing based associative memory for complex dynamical attractors
Authors: Lai Ying-Cheng , Kong Ling-Wei , Brewer Gene 
Published: October 2023

Abstract

Traditional neural-network models of associative memories were for storing and retrieving static patterns. We develop reservoir-computing based memories for complex dynamical attractors, under two common recalling scenarios in neuropsychology: location-addressable with an index channel and context-addressable without such a channel. We demonstrate that, for location-addressable retrieval, a single reservoir computing machine can memorize a large number of periodic and chaotic attractors, each retrievable with a specific index value. We articulate control strategies to achieve successful switching among the attractors. An algebraic scaling law between the number of stored attractors and the reservoir-network size is uncovered. For context-addressable retrieval, we exploit multistability with cue signals, where the stored attractors coexist in the high-dimensional phase space of the reservoir network. As the length of the cue signal increases through a critical value, a high success rate can be achieved. The work provides foundational insights into developing long-term memories for complex dynamical patterns.
Stress and Resilience Education in the Top 50 U.S. Medical Schools
Authors: Roos Lydia G. , Lee Jang H. , Thyne Shannon M. , Bhushan Devika , Slavich George M. 
Published: October 2023

Abstract
Substantial research points to psychosocial stressors as being very common, key drivers of lifelong health disparities. Given this evidence base, it should follow that medical schools provide focused education around stress and adversity, resilience (including resilience-promoting interventions), health equity, and integrative frameworks for understanding health and treating disease. To determine whether this is occurring, we systematically evaluated course descriptions for 50 of the top primary care-focused medical school programs in the United States, as judged by U.S. News and World Report. We calculated the percent of courses addressing each topic, evaluated whether the courses were elective or required, and re-ranked the programs based on their coverage of the above-described topics. Of 227 identified, 89.4% addressed equity, but only 7.0% discussed an integrative framework for understanding health and only 6.6% discussed stress, adversity, or resilience. No programs specifically mentioned life stress, toxic stress, or social stratification. Moreover, only 4.0% of courses were both required and devoted specifically to these topics. Current medical school curricula are thus leaving our future physician workforce ill-equipped to understand and treat stress-related disease. Expanding education on these topics will be important for addressing health inequities.