bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-cultures of cerebellar slices from mice with different reelin genetic backgrounds as a model to study cortical lamination
Authors: Merighi Adalberto , Lossi Laura 
Published: October 2023

Background: Reelin has fundamental functions in the developing and mature brain. Its absence gives rise to the Reeler mouse phenotype. In reln(-/-) mutants, neurons are mispositioned in layered brain areas such as the cerebellar cortex. We demonstrate that in cultured cerebellar slices, one can reduce the number of animals and use a non-recovery procedure to analyze the effects of Reelin on the migration of Purkinje neurons (PNs). Methods: We generated mouse hybrids (L7-GFP reln F1/) with GFP-tagged PNs, directly visible under fluorescence microscopy. We cultured singularly or in combination the slices from mice with different reln genotypes and used Voronoi tessellation and geographic information systems (GIS)-based spatial statistics to validate microscopic observations. Results: In co-cultured slices from reln(-/-) mice, Voronoi polygons were larger than in single-cultured slices of the same genetic background but smaller than in slices of reln(+/-) animals, thus indicating a rearrangement of the cortical architecture toward normality. The mean roundness factor, area disorder, and roundness factor homogeneity differed when slices from reln(-/-) mice were cultivated singularly or co-cultivated with slices from reln(+/-) mice. Analysis of Central Feature, Mean Center, Median Center, Directional Distribution, Standard Distance, Average Nearest Neighbor, Getis-Ord General G, Ripley’s K function, Global Moran’s I, Anselin Local Moran’s I, and Getis-Ord G* were fully supportive of Voronoi’s results giving further insight on the role of Reelin in cerebellar development. Our approach demonstrated mathematically the transition from the clustered organization of the PNs in the absence of Reelin to a layered structure when the protein is supplied ex vivo. Conclusions: Neurobiologists are the primary target users of this 3Rs approach. They should adopt it to study and manipulate ex vivo the activity of a bioactive protein (scientific perspective), the potential reduction (up to 20%) of the animals used, and the avoidance of severe surgery (3Rs perspective).
Indoor environmental quality in schools: NOTECH solution vs. standard solution
Authors: Volf Carlo , Martiny Klaus , Andersen Mathias , Engberg Pallesen Bodil 
Published: October 2023

Background: In many Danish schools, the indoor environmental quality (IEQ) is challenging and studies document a poor IEQ in a majority of existing schools. Municipalities cannot afford comprehensive renovations and expensive mechanical ventilation solutions, hence public schools often suffer from poor indoor environment conditions. This study tests a new façade based, demand-controlled ventilation solution called NOTECH in the renovation of school. The study tests NOTECH vs. existing mechanical ventilation solution, comparing performance of both solutions at Skovbrynet Skole in Denmark. Methods: The project implements and investigates the effect of the NOTECH solution in a primary school classroom, comparing it to a similar classroom with conventional, mechanical ventilation. Methodically, indoor environmental quality and energy performance is monitored in the two identical classrooms during one school year 2018 - 2019. Results: The results show that both systems keep the conditions within acceptable limits and CO2 levels below 1000 ppm, which is the requirement according to the Danish Building Regulations. In terms of costs, the NOTECH system has a lower overall cost than the mechanical ventilation system, with total estimated costs for installation, heating, electricity and maintenance amounting to approximately 35% of the mechanical system’s costs. Finally, the results show that the NOTECH solution has a smaller embedded CO2 footprint for building materials, reducing the estimated carbon load by 95% compared to the mechanical ventilation solution. Conclusions: The performance of the two systems roughly is the same in relation to the indoor environmental quality, temperature, CO2 levels and relative air-humidity. Costs for implementation, energy consumption for heating and CO2 footprint for building materials however, are significantly lower for the NOTECH solution, compared to the mechanical solution.
Antibacterial and anti-virulence potential of plant phenolic compounds against Vibrio parahaemolyticus
Authors: Vazquez-Armenta F. Javier , Aros-Corrales M. Olivia , Alvarez-Ainza M. Lizeth , Bernal-Mercado A. Thalia , Ayala-Zavala J. Fernando , Ochoa-Leyva Adrian , Lopez-Zavala A. Alexis 
Published: October 2023

Background: Vibrio parahaemolyticus is a pathogenic bacterium that affects shrimp aquaculture; its infection can lead to severe production losses of up to 90%. On the other hand, plant phenolic compounds have emerged as a promising alternative to combat bacterial infections. The antibacterial and anti-virulence activity of the plant phenolic compounds quercetin, morin, vanillic acid, and protocatechuic acid against two strains of V. parahaemolyticus (Vp124 and Vp320) was evaluated. Methods: The broth microdilution test was carried out to determine phenolic compounds' antibacterial activity. Moreover, the biofilm-forming ability of V. parahaemolyticus strains in the presence of phenolic compounds was determined by total biomass staining assay using the cationic dye crystal violet. The semisolid agar displacement technique was used to observe the effect of phenolic compounds on the swimming-like motility of V. parahaemolyticus. Results: Results showed that phenolic compounds inhibited both strains effectively, with minimum inhibitory concentrations (MICs) ranging from 0.8 to 35.03 mM. Furthermore, at 0.125 – 0.5 × MIC of phenolic compounds, V. parahaemolyticus biofilms biomass was reduced by 63.22 – 92.68%. Also, quercetin and morin inhibited the motility of both strains by 15.86 – 23.64% (Vp124) and 24.28 – 40.71% (Vp320). Conclusions: The results suggest that quercetin, morin, vanillic, and protocatechuic acids may be potential agents for controlling V. parahaemolyticus.
Human movement patterns of farmers and forest workers from the Thailand-Myanmar border
Authors: Tun Sai Thein Than , Min Myo Chit , Aguas Ricardo , Fornace Kimberly , Htoo Gay Nay , White Lisa J. , Parker Daniel M. 
Published: October 2023

Background: Human travel patterns play an important role in infectious disease epidemiology and ecology. Movement into geographic spaces with high transmission can lead to increased risk of acquiring infections. Pathogens can also be distributed across the landscape via human travel. Most fine scale studies of human travel patterns have been done in urban settings in wealthy nations. Research into human travel patterns in rural areas of low- and middle-income nations are useful for understanding the human components of epidemiological systems for malaria or other diseases of the rural poor. The goal of this research was to assess the feasibility of using GPS loggers to empirically measure human travel patterns in this setting, as well as to quantify differing travel patterns by age, gender, and seasonality among study participants. Methods: In this pilot study we recruited 50 rural villagers from along the Myanmar-Thailand border to carry GPS loggers for the duration of a year. The GPS loggers were programmed to take a time-stamped reading every 30 minutes. We calculated daily movement ranges and multi-day trips by age and gender. We incorporated remote sensing data to assess patterns of days and nights spent in forested or farm areas, also by age and gender. Results: Our study showed that it is feasible to use GPS devices to measure travel patterns, though we had difficulty recruiting women and management of the project was relatively intensive. We found that older adults traveled farther distances than younger adults and adult males spent more nights in farms or forests. Conclusion: The results of this study suggest that further work along these lines would be feasible in this region. Furthermore, the results from this study are useful for individual-based models of disease transmission and land use.
Biological activities and chemical characterization of Lavandula angustifolia essential oil from Seraïdi, Northeastern Algeria
Authors: HADJ MOUSSA ABDEL HAKIM , BENALIOUCHE FOUZIA , SBARTAI IBTISSEM , SBARTAI HANA 
Published: October 2023

Abstract. Hadj Moussa AH, Benaliouche F, Sbartai I, Sbartai H. 2023. Biological activities and chemical characterization of
Lavandula angustifolia essential oil from Seraïdi, Northeastern Algeria. Biodiversitas 24: 4535-4543. The objectives of this study are to examine the chemical composition of essential oil (EO) extracted by hydrodistillation from the flowering tops of dry lavender (Lavandula angustifolia) harvested in the town of Seraïdi in Northeastern Algeria and evaluate its antifungal, antibacterial, and antioxidant potential. The chemical analysis of this essential oil was carried out by gas chromatography coupled with mass spectrophotometry (GC-MS). The antimicrobial activity was evaluated by dilution method in solid and liquid medium, and the antioxidant activity was carried out using the DPPH° radical scavenging assay. It revealed the presence of 20 compounds representing the general component of this oil with a yield of 1.71%. This oil is composed mainly of Linalool (31.27%), followed by Camphor (16.21%), Linalool Oxide (11.98%), and Linalyl Acetate (11.93%). Other constituents were identified at relatively medium [2-Furarmethanol (7.49%), 1,8-Cineole/eucalyptol (6.76%), Borneol (5.34%)] and low contents [1-Hexyl butyrate (1.25%)]. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of lavender EO against Fusarium roseum were 3000 µg/mL and 4000 µg/mL, respectively. Lavender OE had moderate antibacterial activity against Pseudomonas savastanoi. The antioxidant activity of lavender EO was lower than that of vitamin C.
Macroeconomic Strategies and Policies Impacting the Decarburization of Economies in the Member Countries of the European Union
Authors: CIOBANU Ghenadie , GROAZNICU Luminița Popescu , RĂDUCANU Dragoș , GOMBOS Carol Cristina 
Published: October 2023

In the context of the strategic development objectives of the European Union for the next strategic cycle until 2030, a priority issue is the decarburization of the economy, oriented towards the design and monitoring of strategies in order to achieve the objectives regarding climate change, the simultaneous stimulation of economic growth and ensuring social cohesion. A special place belongs to the progress of climate change, which requires progressive steps in the implementation of strategies and policies for all member countries of the EU. We propose in this article to focus on the application of decarburization strategies and policies in the countries of the European Union with an emphasis on Romania, with a necessary approach to social inclusion issues. The development of proposals for efficient strategies for the decarburization of economies in EU, the comparative analysis between the diversification of macroeconomic policies and instruments with different evaluation criteria, having as an essential support - the digitalization tools, which will accelerate the process of decarburization of the countries' economy EU. We propose the development of decarburization strategies based on a mix of policies focused on establishing emission prices; standards and regulations; the implementation of capital, labor and innovation reallocation policies.
Key words: Decarburization of the economy, strategies and policies, digitalization, innovation
Avalanche Danger on the Highways of the Samegrelo-Zemo Svaneti Region (Georgia)
Authors: Pipia Mikheil , Gorgijanidze Sophio , Beglarashvili Nazibrola , Kobakhidze Natela , Jintcharadze Gocha 
Published: October 2023

The importance of highways in the mountainous regions of Georgia is very great for the socio-economic development of the country. Such natural disasters as snow avalanches significantly damage the road infrastructure, create delays in movement, often cause human casualties. Therefore, in order to implement preventive measures, the study of avalanche hazards on highways is of great importance. Mestia municipality of Samegrelo-Zemo Svaneti region (Zemo Svaneti) is characterized by mountainous and high-mountain terrain, snow avalanches are frequent, including on sections of highways.
Avalanche hazards on highways of Mestia municipality of Samegrelo-Zemo Svaneti region and climatic factors of their occurrence are studied. The data of the Mestia meteorological station on air temperature and solid precipitation covering the years 1961-2021 have been processed. A geo-informational map of the avalanche danger of highways in the Samegrelo-Zemo Svaneti region has been compiled.
Keywords: Snow avalanches, climate, natural disaster, highways, geoinformation map
Have Hungarian districts become more resilient? – A comparison of the 2014 and 2020 Baseline Resilience Indicators for Communities (BRICs)
Authors: Csizovszky Anna 
Published: October 2023

Recent crises have put resilience at the center of sustainability and territorial studies. Enhancing resilience plays a crucial role in sustainable regional development and calls for knowledge of local vulnerabilities as well as capacity for absorption, adaptation, and transformation. Community resilience refers to the ability of different regional levels to adjust their socioeconomic systems to manage the adverse effects of shocks and stresses and to be able to provide adaptive responses. So far, studies have focused mainly on specific areas, which is difficult to apply in the context of successive crises and makes it more challenging to set development priorities.
This research adopts the Baseline Resilience Indicators for Communities (BRIC) framework and focuses on changes between 2014 and 2020. The community resilience assessment of Hungarian districts revealed quite a homogenous performance concerning the overall score but showed high heterogeneity concerning the five resilience dimensions evaluated. Over time, the indicators generally increased in the social and economic domains and decreased in the community domain. Besides, rapid suburbanization processes around cities contributed to lower overall performances indicating the necessity of action.
Keywords: community resilience, BRIC, regional pattern, Hungary, spatiotemporal analysis
The Spread of Snow Avalanches at Transport Junctions in the Mountainous Regions of Georgia
Authors: Gorgidjanidze Sophio , Beglarashvili Nazibrola , Pipia Mikheil , Kobakhidze Natela 
Published: October 2023

The snow avalanche has an insidious character, it can be repeated once in 100-200 years and cause great harm to the population of the mountainous region. There are many examples of damage caused by avalanches. In the 20th century, several thousand soldiers were died by avalanches in the Alps during the First World War, on the Austrian-Italian front. There are many examples of them not only in the Alps but also in the mountainous regions of Georgia. Frequently, snow avalanches block important transportation routes that connect people and countries.
In general, 56% of the territory of Georgia is covered with avalanche-prone slopes. Based on existing examples, it is important to record and study them, because snow avalanches are characterized by repetition. Examples of this are Svaneti, Racha and Mtianeti (regions of Georgia). It is worth noting the Mleta-Kazbegi section road (Georgia's military road), where there is a danger of avalanches every year, which is the subject of our research. The highway of Georgia is a transport hub of international importance. Therefore, observation of avalanches, study and carrying out various measures is a necessary prerequisite in order to avoid damage caused by avalanches.
Keywords: Mountainous regions, avalanche danger, climate, natural phenomena, transport infrastructure
Sustainability in a Sand-Depleted World: Identifying Barriers to Adoption of Sand Substitutes
Authors: Zadeh Adel A. , Peng Yunxin , Puffer Sheila M. 
Published: October 2023

The global sand crisis has gained considerable attention among environmentalists over the last few years, and the United Nations has proposed some initiatives to reduce the use of river sand. Despite the existence of a number of promising sustainable alternatives to alluvial sand, there has been little effort to implement those initiatives in the construction industry. This paper attempts to develop a better understanding of barriers and challenges related to the adoption of sustainable substitutes for sand in the construction industry. An online survey designed by the authors was distributed among construction industry professionals located in 35 US states and 7 Canadian provinces. The findings from 344 respondents show that different stakeholders in construction have different priorities and concerns when it comes to sustainable sand substitutes, with some focusing more on the technical and practical aspects, while others focus more on the long-term and environmental aspects.
Keywords: : Construction industry, sand crisis, barriers, awareness, sand substitutes
School for Sustainable Living: Assessing the Energy Related Topics Among Young People
Authors: Breskvar Uros 
Published: October 2023

We associate modern man with a sustainable way of life. The very concept of sustainability is closely linked to the energy we need for our lives. The amount of energy is limited by planet Earth itself and by technology for transformation/capturing energy. Man has always strived to have as much energy as possible, as it significantly affects the quality of life. With a population growth and slowly decreasing reserves of fossil fuels, it is increasingly important to make the most of a given energy and move to clean energy sources. The article conducted research on how well young people know the basic energy values for everyday needs and how familiar they are with current technologies that enable better use of energy. According to the results of the research, suggestions are made for improving general energy literacy among young people.
Keywords: energy, sustainable competences, education, knowledge examination
Exploration of Healthcare Sensitive Systems and Services for Supporting Ambient Assisted Living of Diversified Social Groups in South Korea
Authors: Chang Seongju , Nam Hyunjae 
Published: October 2023

Different social groups normally have specific sets of requirements and priorities in relation to their surrounding living environment. In our research, we conducted an extensive survey on the preferred conditions and demands for four different domains of daily life: healthcare, environment, activity, and information. Carefully composed sets of questionnaires were distributed to 800 people representing diversified social groups in South Korea classified by gender, age, occupation, co-residents, home location, housing style, economic level, and chronic disease. Their responses were collected and analyzed through cross-tabulation to identify characteristic preference patterns concerning their immediate housing unit, residential cluster, and close neighborhood environment and services. Based on the extracted socio-environmental preferences of the different social groups in South Korea, we developed a web-based information system called AALPSS (Ambient Assisted Living Pattern Support System), which includes a total of 100 selected healthcare-sensitive system/service patterns to support the optimum design and operation of artifacts and facilitate the promotion of a healthy silver life. Each pattern is organized based on Christopher Alexander's 'Pattern Language' format and can be browsed and rated by different online users with diverse social profiles. This information system, therefore, has the potential to evolve into a better-tuned healthcare-related system/service decision support system, enhancing social sustainability within a community.
Keywords: social group, ambient assisted living, preferred life pattern, healthcare sensitive environment, online information system
Research of Adverse Social Effects and Prevention Challenges in the Context of Sustainable Societal Development
Authors: Kipāne Aldona , Vilks Andrejs 
Published: October 2023

The sustainable development of society is determined by activities aimed at ensuring the well-being of society, integrated and balanced evolution of the environment and economy, at the same time, without jeopardising the possibilities of meeting the needs of future generations. Improving the quality of life of every member of society by improving economic activities, promoting social cohesion and security are of great importance. In today’s society, crime, corruption and other adverse social phenomena have a major impact on the sustainable development of society by reducing investment in the development and implementation of full-fledged growth programmes. During the crisis and post-crisis period, social pathologies are growing and their adverse effects are increasing too. The true indicators of their prevalence and negative impact are objectively sufficiently not known. Adverse social phenomena have a particularly negative impact on the sustainable development of society, which has various and diverse forms of influence. Large financial, material, intellectual and other resources, which could be used in the sustainable development of society, are accumulated in the research of adverse phenomena, assessment of their impact, combating and prevention.
Keywords: development, prevention, research, social effects, social sustainability.
Economic impact of productive use of renewable energy: A case of a women-collective from rural Maharashtra (India)
Authors: Ingole Chandrakant K. 
Published: October 2023

Development activities and energy access are intertwined; however, they also contribute towards climate change. Therefore, climate friendly activities are needed to be promoted to ensure socio-economic development. Productive use of renewable energy for income generation is an emerging area of development in developing countries and is under-explored; therefore, there is a need to study the impact of such interventions on income generation. In this context, this paper attempts to estimate the impact of a renewable energy based, women managed collective from rural Maharashtra (India) on the income of beneficiaries using interview-based survey design. An analysis of the data indicates a significant change in beneficiaries’ income with associated multiplier effect vis-à-vis non-beneficiaries’, depicting such initiatives have potential to effect socio-economic development while being environment friendly. This is vital as India foresees an important role for renewable energy to address development issues, rural infrastructure gaps, and climate goals.
Keywords: Climate Change, Sustainable Development, Renewable Energy, Collective, Women Empowerment