bluuebunny/crossref_metadata_2025_split huggingface dataset:
NILAI PENDIDIKAN KARAKTER DENGAN DIRI SENDIRI DALAM NOVEL SEKUNTUM CINTA UNTUK ADELIA KARYA EVA LIANA
Authors: Janah Nor , Komalasari Ida , Aswadi Dana 
Published: October 2023

Pendidikan karakter dalam konteks sekarang sangat relevan untuk mengatasi krisis moral yang sedang melanda negara kita. Krisis-krisis moral itu adalah: pergaulan bebas; maraknya angka kekerasan anak-anak dan remaja; kejahatan terhadap teman; pencurian; tawuran pelajaran; kecurangan ujian; penyalahgunaan obat-obatan; pornografi; pelecehan seksual; tindak korupsi, mengunsumsi bahkan menjadi pencandu narkoba, menjadi kelompok geng motor yang anarkis, dan lain-lain. Bisa kita amati dalam kehidupan sehari-hari.
Penelitian ini, peneliti menggunakan pendekatan sosiologi sastra. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian kepustakaan. Disebut penelitian kepustakaan karena data-data atau bahan yang diperlukan dalam menyelesaikan penelitian ini berasal dari perpustakaan baik berupa buku, jurnal, kamus, dokumen, dan majalah. Metode adalah cara bagaimana penelitian itu dilakukan. Metode yang digunakan dalam penelitian ini adalah metode deskriptif analisis.
Hasil penelitian nilai-nilai pendidikan karakter dengan diri sendiri dalam novel Sekuntum Cinta untuk Adelia Karya Eva Liana mencakup nilai sebagai berikut. (1) jujur, (b) disiplin, (c) kerja keras, (d) kreatif, (e) mandiri, (f) gemar membaca, dan (g) tanggung jawab.
PENINGKATAN KETERAMPILAN MEMBACA PERMULAAN DENGAN MENGGUNAKAN MEDIA KARTU KATA PADA SISWA KELAS 1 SD NEGERI RUMINTIN 2 TAHUN 2022
Authors: Normaniah  
Published: October 2023

Penelitian ini bertujuan untuk meningkatkan keterampilan membaca permulaan dengan menggunakan media kartu kata pada siswa kelas 1 SD Negeri Rumintin 2 Kecamatan Tapin Selatan Kabupaten Tapin. Jenis penelitian ini adalah Penelitian Tindakan Kelas kolaborasi yang dilakukan sebanyak dua siklus. Desain penelitian menggunakan model Kemmis Mc. Teggart dengan subjek penelitian siswa kelas 1 yang berjumlah 16 siswa. Teknik pengumpulan data menggunakan 1) tes membaca lisan dan tes tertulis memahami bacaan, 2) observasi, dan 3) dokumentasi. Teknik analisis data menggunakan analisis statistik deskriptif yaitu dengan mencari rerata. Indikator keberhasilan siswa yang harus dicapai dengan rerata kelas 70 dan ketuntasannya 80%.
Hasil penelitian menunjukkan bahwa pembelajaran membaca permulaan dengan menggunakan media kartu kata dapat meningkatkan keterampilan membaca siswa kelas 1 SD Negeri Rumintin Keterampilan membaca pada pra siklus sebesar 60,31 dan ketuntasanya 37,5%, pada siklus I meningkat menjadi 67,34 dengan ketuntasan 56,25%, peningkatan pada siklus II 72,66 dengan ketuntasan 93,75%. Pada tindakan ini keterampilan membaca siswa dengan lafal, intonasi dan membaca memahami meningkat hingga mencapai kriteria ketuntasan yang ditetapkan yaitu dengan rata-rata kelas 70 dan ketuntasan kelas 80%. Pada siklus I digunakan kartu kata dengan ukuran 13 x 6 cm dan setiap kata dengan satu warna, pada siklus II digunakan kartu kata yang lebih lebih besar 18 x 6 cm dan setiap kata terdapat pemengaalan kata yang dipisahkan dengan warna yang berbeda.
Konstruksi Pendengar Radio pada Masyarakat Indonesia (Studi Kasus pada Aplikasi Noice)
Authors: Hayati Kumala , Ariestanty Camelia 
Published: October 2023

Kehadiran teknologi telah mengubah kehidupan manusia. Teknologi membuat hidup manusia menjadi semakin mudah. Teknologi telah membuat cara pendengar menikmati radio juga turut berubah. Lahirnya aplikasi Noice membuat radio bertransformasi menjadi bentuk digital. Tulisan ini akan menjelaskan bagaimana radio mengonstruksi pendengarnya dengan bentuk teknologi yang baru. Metode penelitian dalam tulisan ini adalah observasi dan wawancara mendalam pada beberapa informan. Hasil dari tulisan ini adalah pendengar mempunyai pilihan cara untuk mendengarkan radio yaitu melalui perangkat yang telah terpasang di mobil dan melalui gadget masing – masing (streaming). Pilihan konten juga penting bagi pendengar karena menyesuaikan dengan tujuan dari aktivitas mendengarkan radio itu sendiri. Hadirnya teknologi radio dalam bentuk aplikasi berbasis internet dapat memengaruhi perilaku, membentuk persepsi dan mengelola tindakan khalayak.
Kata kunci : konvergensi, digitalisasi, radio, determinisme teknologi
Long-term clinical outcomes for patients with uncrossable patent foramen ovale
Authors: Shin Yonghoon , Jang Albert Youngwoo , Won Yoonsun , Yang Taeil , Kim Joohan , Lee Joonpyo , Seo Jeongduk , Kim Minsu , Oh Pyung Chun , Chung Wook-Jin , Moon Jeonggeun , Kang Woong Chol 
Published: October 2023

IntroductionPatent foramen ovale (PFO) closure is performed in selected patients with cryptogenic stroke to prevent recurrence. The prognosis of patients with uncrossable PFO after failed guidewire or catheter passage during the procedure remains unknown. We compared the long-term prognosis between uncrossable PFO and successful PFO closure in patients with high-grade PFO shunts.MethodsWe analyzed patients who underwent PFO closure for stroke or transient ischemic attack (TIA) prevention at Gachon University Gil Medical Center between April 2010 and March 2022. The primary outcome was a composite of recurrent stroke or TIA. Secondary outcomes included stroke, TIA, all-cause death, and a composite of stroke, TIA, and all-cause death.ResultsOf 286 patients, 245 were included in the analysis after excluding those with transseptal puncture technique usage or concurrent atrial septal defect. Among them, 82 had uncrossable PFO, and 163 underwent successful PFO closure. Large shunts were more prevalent in the PFO closure group compared to the uncrossable PFO group (62.0% vs. 34.1%, P &lt; 0.001), and resting shunts were also more common in the PFO closure group (17.8% vs. 2.4%, P &lt; 0.001). Stroke or TIA occurred in 2 patients (2.4%) in the uncrossable PFO group and 8 patients (4.9%) in the PFO closure group (hazard ratio, 1.44; 95% confidence interval, 0.30–6.81; P = 0.647). Additionally, no disparities in the occurrence of stroke or TIA were found in subgroups divided by baseline characteristics, RoPE score, or shunt grade.ConclusionClinical outcomes for patients with uncrossable PFO seem similar to those with successful PFO closure.
PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages
Authors: Witzel Roman , Block Annika , Pollmann Solvey , Oetzel Leandra , Fleck Fenja , Bonaterra Gabriel A. , Kinscherf Ralf , Schwarz Anja 
Published: October 2023

BackgroundPituitary adenylate cyclase-activating polypeptide (PACAP) acts as an anti-atherogenic neuropeptide and plays an important role in cytoprotective, as well as inflammatory processes, and cardiovascular regulation. Therefore, the aim of this study is to investigate the regulatory effects of PACAP and its receptor VPAC1 in relation to inflammatory processes and lipid homeostasis in different macrophage (MΦ) subtypes.MethodsTo investigate the role of PACAP deficiency in the pathogenesis of atherosclerosis under standard chow (SC) or cholesterol-enriched diet (CED) in vivo, PACAP−/− mice were crossbred with ApoE−/− to generate PACAP−/−/ApoE−/− mice. Lumen stenosis in the aortic arch and different MΦ-subtypes were analyzed in atherosclerotic plaques by quantitative immunohistochemistry. Undifferentiated bone marrow-derived cells (BMDC) from 30-weeks-old ApoE−/− and PACAP−/−/ApoE−/− mice were isolated, differentiated into BMDM1- and BMDM2-MΦ, and incubated with oxidized low-density lipoprotein (oxLDL). In addition, PMA-differentiated human THP-1 MΦ were further differentiated into M1-/M2-MΦ and subsequently treated with PACAP38, the VPAC1 agonist [(Ala11,22,28)VIP], the antagonist (PG 97–269), and/or oxLDL. Uptake/accumulation of oxLDL was analyzed by oxLDL-DyLight™488 and Bodipy™ 493/503. The mRNA expression was analyzed by qRT-PCR, protein levels by Western blot, and cytokine release by ELISA.ResultsIn vivo, after 30 weeks of SC, PACAP−/−/ApoE−/− mice showed increased lumen stenosis compared with ApoE−/− mice. In atherosclerotic plaques of PACAP−/−/ApoE−/− mice under CED, immunoreactive areas of VPAC1, CD86, and CD163 were increased compared with ApoE−/− mice. In vitro, VPAC1 protein levels were increased in PACAP−/−/ApoE−/− BMDM compared with ApoE−/− BMDM, resulting in increased TNF-α mRNA expression in BMDM1-MΦ and decreased TNF-α release in BMDM2-MΦ. Concerning lipid homeostasis, PACAP deficiency decreased the area of lipid droplets in BMDM1-/M2-MΦ with concomitant increasing adipose differentiation-related protein level. In THP-1 M1-/M2-MΦ, the VPAC1 antagonist increased the uptake of oxLDL, whereas the VPAC1 agonist decreased the oxLDL-induced intracellular triglyceride content.ConclusionOur data suggest that PACAP via VPAC1 signaling plays an important regulatory role in inflammatory processes in atherosclerotic plaques and in lipid homeostasis in different MΦ-subtypes, thereby affecting foam cell formation. Therefore, VPAC1 agonists or PACAP may represent a new class of anti-atherogenic therapeutics.
Novel mutations in the SGCA gene in unrelated Vietnamese patients with limb-girdle muscular dystrophies disease
Authors: Chung Tran Nam , Lien Nguyen Thi Kim , Ta Thanh Dat , Nguyen Van Hung , Tran Huy Thinh , Van Tung Nguyen , Xuan Nguyen Thi , Huy Hoang Nguyen , Tran Van Khanh 
Published: October 2023

Background: Limb-girdle muscular dystrophy (LGMD) is a group of inherited neuromuscular disorders characterized by atrophy and weakness in the shoulders and hips. Over 30 subtypes have been described in five dominant (LGMD type 1 or LGMDD) and 27 recessive (LGMD type 2 or LGMDR). Each subtype involves a mutation in a single gene and has high heterogeneity in age of onset, expression, progression, and prognosis. In addition, the lack of understanding of the disease and the vague, nonspecific symptoms of LGMD subtypes make diagnosis difficult. Even as next-generation sequencing (NGS) genetic testing has become commonplace, some patients remain undiagnosed for many years.Methods: To identify LGMD-associated mutations, Targeted sequencing was performed in the patients and Sanger sequencing was performed in patients and family members. The in silico analysis tools such as Fathmm, M-CAP, Mutation Taster, PolyPhen 2, PROVEAN, REVEL, SIFT, MaxEntScan, Spliceailookup, Human Splicing Finder, NetGene2, and Fruitfly were used to predict the influence of the novel mutations. The pathogenicity of the mutation was interpreted according to the ACMG guidelines.Results: In this study, six patients from four different Vietnamese families were collected for genetic analysis at The Center for Gene and Protein Research and The Department of Molecular Pathology Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam. Based on clinical symptoms and serum creatine kinase (CK) levels, the patients were diagnosed with limb-girdle muscular dystrophies. Five mutations, including four (c.229C&gt;T, p.Arg77Cys; exon one to three deletion; c.983 + 5G&gt;C; and c.257_258insTGGCT, p.Phe88Leufs*125) in the SGCA gene and one (c.946-4_946-1delACAG) in the CAPN3 gene, were detected in six LGMD patients from four unrelated Vietnamese families. Two homozygous mutations (c.983 + 5G&gt;C and c.257_258insTGGCT) in the SGCA gene were novel. These mutations were identified as the cause of the disease in the patients.Conclusion: Our results contribute to the general understanding of the etiology of the disease and provide the basis for definitive diagnosis and support genetic counseling and prenatal screening.
Extracellular proteins as potential biomarkers in Sepsis-related cerebral injury
Authors: Dong Jinlan , Wang Shuang , Hu Zhonghua , Gong Li 
Published: October 2023

BackgroundSepsis can cause brain damage known as septic encephalopathy (SAE), which is linked to higher mortality and poorer outcomes. Objective clinical markers for SAE diagnosis and prognosis are lacking. This study aimed to identify biomarkers of SAE by investigating genes and extracellular proteins involved in sepsis-induced brain injury.MethodsExtracellular protein differentially expressed genes (EP-DEGs) from sepsis patients’ brain tissue (GSE135838) were obtained from Gene Expression Omnibus (GEO) and evaluated by protein annotation database. The function and pathways of EP-DEGs were examined using GO and KEGG. Protein-protein interaction (PPI) networks were built and crucial EP-DEGs were screened using STRING, Cytoscape, MCODE, and Cytohubba. The diagnostic and prognostic accuracy of key EP-DEGs was assessed in 31 sepsis patients’ blood samples and a rat cecal ligation and puncture (CLP)-induced sepsis model. Cognitive and spatial memory impairment was evaluated 7-11 days post-CLP using behavioral tests. Blood and cerebrospinal fluid from 26 rats (SHAM n=14, CLP n=12) were collected 6 days after CLP to analyze key EP-DEGs.ResultsThirty-one EP-DEGs from DEGs were examined. Bone marrow leukocytes, neutrophil movement, leukocyte migration, and reactions to molecules with bacterial origin were all enhanced in EP-DEGs. In comparison to the sham-operated group, sepsis rats had higher levels of MMP8 and S100A8 proteins in their venous blood (both p&lt;0.05) and cerebrospinal fluid (p=0.0506, p&lt;0.0001, respectively). Four important extracellular proteins, MMP8, CSF3, IL-6, and S100A8, were identified in clinical peripheral blood samples. MMP8 and S100A8 levels in the peripheral blood of sepsis patients were higher in SAE than in non-SAE. In comparison to MMP8, S100A8 had a higher area under the curve (AUC: 0.962, p&lt;0.05) and a higher sensitivity and specificity (80% and 100%, respectively) than MMP8 (AUC: 0.790, p&lt;0.05). High levels of S100A8 strongly correlated with 28-day mortality and the Glasgow Coma Scale (GCS) scores.ConclusionThe extracellular proteins MMP8, CSF3, IL-6, and S100A8 may be crucial in the pathophysiology of SAE. S100A8 and MMP8 are possible biomarkers for SAE’s onset and progression. This research may help to clarify the pathogenesis of SAE and improve the diagnosis and prognosis of the disease.
Efficacy and safety of immunosuppressive agents for adults with lupus nephritis: a systematic review and network meta-analysis
Authors: Jiang Nan , Jin Shangyi , Yu Chen , Zhao Jiuliang , Wang Qian , Tian Xinping , Li Mengtao , Zeng Xiaofeng 
Published: October 2023

IntroductionVarious immunosuppressive regimens have been developed for the treatment of lupus nephritis (LN). This study aimed to compare the efficacy and safety of immunosuppressive regimens in adults with LN.MethodsWe systematically searched the PubMed, Embase, and Cochrane Central Register of Controlled Trials databases, including conference proceedings, trial registries, and reference lists, from inception until July 10, 2022. The effects of treatment were compared and ranked using the surface under the cumulative ranking curve (SUCRA). The primary endpoint was total remission. The secondary endpoints were complete remission, systemic lupus erythematosus disease activity index (SLEDAI), relapse, all-cause mortality, end-stage renal disease (ESRD), infection, herpes zoster, ovarian failure, myelosuppression, and cancer.ResultsSixty-two trials reported in 172 studies involving 6,936 patients were included in the network meta-analysis. The combination of tacrolimus (TAC), mycophenolate mofetil (MMF), and glucocorticoid (GC) provided the best result for the total remission rate (SUCRA, 86.63%) and SLEDAI (SUCRA, 91.00%), while the combination of voclosporin (VCS) , MMF and GC gave the best improvement in the complete remission rate (SUCRA, 90.71%). The combination of cyclophosphamide (CYC), MMF and GC was associated with the lowest risk of relapse (SUCRA, 85.57%) and cancer (SUCRA, 85.14%), while the combination of obinutuzumab (OTB), MMF and GC was associated with the lowest risk of all-cause mortality (SUCRA, 84.07%). Rituximab (RTX) plus MMF plus GC was associated with the lowest risk of ESRD (SUCRA, 83.11%), while the risk of infection was lowest in patients treated with azathioprine (AZA) plus CYC plus GC (SUCRA, 68.59%). TAC plus GC was associated with the lowest risk of herpes zoster (SUCRA, 87.67%) and ovarian failure (SUCRA, 73.60%). Cyclosporine (CsA) plus GC was associated with the lowest risk of myelosuppression (SUCRA, 79.50%), while AZA plus GC was associated with the highest risk of myelosuppression (SUCRA, 16.25%).DiscussionThis study showed that a combination of TAC, MMF and GC was the best regimen for improving the total remission rate. The optimal regimen for specific outcomes should be highlighted for high-risk patients.
Single-cell mass cytometric analysis of peripheral immunity and multiplex plasma marker profiling of non-small cell lung cancer patients receiving PD-1 targeting immune checkpoint inhibitors in comparison with platinum-based chemotherapy
Authors: Neuperger Patrícia , Szalontai Klára , Gémes Nikolett , Balog József Á. , Tiszlavicz László , Furák József , Lázár György , Puskás László G. , Szebeni Gábor J. 
Published: October 2023

IntroductionThe effect of platinum-based chemotherapy (Chem.) and second- or multiple- line immune checkpoint PD-1 blocking therapy by Nivolumab or Pembrolizumab (ICI) was assayed in the peripheral blood of non-small cell lung cancer (NSCLC) patients.MethodsFlow cytometry was used to detect NSCLC-related antigen binding IgG antibodies. The Luminex MagPix multiplex bead-based cytokine/chemokine detecting system was used to quantitatively measure 17 soluble markers in the plasma samples. Single-cell mass cytometry was applied for the immunophenotyping of peripheral leukocytes.ResultsThe incubation of patient derived plasma with human NSCLC tumor cell lines, such as A549, H1975, and H1650, detected NSCLC-specific antibodies reaching a maximum of up to 32% reactive IgG-positive NSCLC cells. The following markers were detected in significantly higher concentration in the plasma of Chem. group versus healthy non-smoker and smoker controls: BTLA, CD27, CD28, CD40, CD80, CD86, GITRL, ICOS, LAG-3, PD-1, PD-L1, and TLR-2. The following markers were detected in significantly higher concentration in the plasma of ICI group versus healthy non-smoker and smoker controls: CD27, CD28, CD40, GITRL, LAG-3, PD-1, PD-L1, and TLR-2. We showed the induction of CD69 and IL-2R on CD4+ CD25+ T-cells upon chemotherapy; the exhaustion of one CD8+ T-cell population was detected by the loss of CD127 and a decrease in CD27. CD19+CD20+, CD79B+, or activated B-cell subtypes showed CD69 increase and downregulation of BTLA, CD27, and IL-2R in NSCLC patients following chemotherapy or ICI.DiscussionPeripheral immunophenotype caused by chemotherapy or PD-1 blocking was shown in the context of advanced NSCLC.
Monocytes and T cells incorporated in full skin equivalents to study innate or adaptive immune reactions after burn injury
Authors: Mulder Patrick P.G. , Vlig Marcel , Elgersma Anouk , Rozemeijer Lotte , Mastenbroek Leonore S. , Middelkoop Esther , Joosten Irma , Koenen Hans J.P.M. , Boekema Bouke K.H.L. 
Published: October 2023

IntroductionThermal injury often leads to prolonged and excessive inflammation, which hinders the recovery of patients. There is a notable absence of suitable animal-free models for investigating the inflammatory processes following burn injuries, thereby impeding the development of more effective therapies to improve burn wound healing in patients.MethodsIn this study, we established a human full skin equivalent (FSE) burn wound model and incorporated human peripheral blood-derived monocytes and T cells.ResultsUpon infiltration into the FSEs, the monocytes differentiated into macrophages within a span of 7 days. Burn-injured FSEs exhibited macrophages with increased expression of HLA-DR+ and elevated production of IL-8 (CXCL8), in comparison to uninjured FSEs. Among the T cells that actively migrated into the FSEs, the majority were CD4+ and CD25+. These T cells demonstrated augmented expression of markers associated with regulatory T cell, Th1, or Th17 activity, which coincided with significant heightened cytokine production, including IFN-γ, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IP-10 (CXCL10), and TGF-β1. Burn injury did not impact the studied effector T cell subsets or cytokine levels.DiscussionCollectively, this study represents a significant advancement in the development of an immunocompetent human skin model, specifically tailored for investigating burn-induced innate or adaptive immune reactions at the site of burn injury.
Transradial intra-aortic catheter looping in the angioplasty of severe intracranial symptomatic arteriosclerotic diseases
Authors: Xu Gang-Qin , Xia Jin-Chao , Cai Dong-Yang , Yang Bo-Wen , Zhao Tong-Yuan , Xue Jiang-Yu , Wang Zi-Liang , Li Tian-Xiao , Gao Bu-Lang 
Published: October 2023

PurposeThis study aims to investigate the effect and feasibility of intra-aortic catheter looping via transradial access in angioplasty for symptomatic intracranial severe (&gt;70%) atherosclerotic stenosis or occlusion of large arteries (SISOLAs).Materials and methodsPatients with SISOLAs who underwent transradial endovascular angioplasty using the catheter looping technique in the ascending aorta were retrospectively enrolled. The clinical data and treatment outcomes were analyzed.ResultsFifteen patients aged 48–71 years were enrolled in this study. Left vertebrobasilar artery occlusion was present in 1 (6.7%) patient, severe left middle cerebral artery stenosis in 7 (46.7%) patients, severe left internal carotid artery (ICA) stenosis of the ophthalmic segment in 4 (26.7%) patients, severe left ICA stenosis of the cavernous segment in 2 (13.3%) patients, and severe right middle cerebral artery stenosis in 2 (13.3%) patients. The arterial stenosis ranged from 70 to 92% (mean 86%) before stenting. The looping of a guiding catheter in the ascending aorta via transradial access for angioplasty was successful in all patients (100%). The vertebral artery intracranial segment occlusion was successfully recanalized, while severe stenosis in the remaining 14 patients was successfully eliminated. After endovascular recanalization, the residual stenosis was reduced by 12–26% (median 18%). No puncture-related complications or surgical-related neurological complications occurred in these patients. In the follow-up angiography conducted on 10 (66.7%) patients after 6–25 months, no in-stent restenosis was detected.ConclusionIntra-aortic guiding catheter looping via transradial access for endovascular angioplasty of SISOLAs is technically safe, feasible, and effective, especially when the transfemoral artery approach is difficult or impossible to undertake.