bluuebunny/crossref_metadata_2025_split huggingface dataset:
P-MSC-derived extracellular vesicles facilitate diabetic wound healing via miR-145-5p/ CDKN1A-mediated functional improvements of high glucose-induced senescent fibroblasts
Authors: Su Jianlong , Wei Qian , Ma Kui , Wang Yaxi , Hu Wenzhi , Meng Hao , Li Qiankun , Zhang Yuehou , Zhang Wenhua , Li Haihong , Fu Xiaobing , Zhang Cuiping 
Published: October 2023

AbstractBackgroundPersistent hyperglycaemia in diabetes causes functional abnormalities of human dermal fibroblasts (HDFs), partially leading to delayed skin wound healing. Extracellular vesicles (EVs) containing multiple pro-healing microRNAs (miRNAs) have been shown to exert therapeutic effects on diabetic wound healing. The present study aimed to observe the effects of EVs derived from placental mesenchymal stem cells (P-MSC-EVs) on diabetic wound healing and high glucose (HG)-induced senescent fibroblasts and to explore the underlying mechanisms.MethodsP-MSC-EVs were isolated by differential ultracentrifugation and locally injected into the full-thickness skin wounds of diabetic mice, to observe the beneficial effects on wound healing in vivo by measuring wound closure rates and histological analysis. Next, a series of assays were conducted to evaluate the effects of low (2.28 x 1010 particles/ml) and high (4.56 x 1010 particles/ml) concentrations of P-MSC-EVs on the senescence, proliferation, migration, and apoptosis of HG-induced senescent HDFs in vitro. Then, miRNA microarrays and real-time quantitative PCR (RT–qPCR) were carried out to detect the differentially expressed miRNAs in HDFs after EVs treatment. Specific RNA inhibitors, miRNA mimics, and small interfering RNA (siRNA) were used to evaluate the role of a candidate miRNA and its target genes in P-MSC-EV-induced improvements in the function of HG-induced senescent HDFs.ResultsLocal injection of P-MSC-EVs into diabetic wounds accelerated wound closure and reduced scar widths, with better-organized collagen deposition and decreased p16INK4a expression. In vitro, P-MSC-EVs enhanced the antisenescence, proliferation, migration, and antiapoptotic abilities of HG-induced senescent fibroblasts in a dose-dependent manner. MiR-145-5p was found to be highly enriched in P-MSC-EVs. MiR-145-5p inhibitors effectively attenuated the P-MSC-EV-induced functional improvements of senescent fibroblasts. MiR-145-5p mimics simulated the effects of P-MSC-EVs on functional improvements of fibroblasts by suppressing the expression of cyclin-dependent kinase inhibitor 1A and activating the extracellular signal regulated kinase (Erk)/protein kinase B (Akt) signaling pathway. Furthermore, local application of miR-145-5p agomir mimicked the effects of P-MSC-EVs on wound healing.ConclusionsThese results suggest that P-MSC-EVs accelerate diabetic wound healing by improving the function of senescent fibroblasts through the transfer of miR-145-5p, which targets cyclin-dependent kinase inhibitor 1A to activate the Erk/Akt signaling pathway. P-MSC-EVs are promising therapeutic candidates for diabetic wound treatment.
A chromosome-level reference genome for the common octopus, Octopus vulgaris (Cuvier, 1797)
Authors: Destanović Dalila , Schultz Darrin T , Styfhals Ruth , Cruz Fernando , Gómez-Garrido Jèssica , Gut Marta , Gut Ivo , Fiorito Graziano , Simakov Oleg , Alioto Tyler S , Ponte Giovanna , Seuntjens Eve 
Published: October 2023

Abstract
Cephalopods are emerging animal models and include iconic species for studying the link between genomic innovations and physiological and behavioral complexities. Coleoid cephalopods possess the largest nervous system among invertebrates, both for cell counts and brain-to-body ratio. Octopus vulgaris has been at the center of a long-standing tradition of research into diverse aspects of cephalopod biology, including behavioral and neural plasticity, learning and memory recall, regeneration, and sophisticated cognition. However, no chromosome-scale genome assembly was available for O. vulgaris to aid in functional studies. To fill this gap, we sequenced and assembled a chromosome-scale genome of the common octopus, O. vulgaris. The final assembly spans 2.8 billion basepairs, 99.34% of which are in 30 chromosome-scale scaffolds. Hi-C heatmaps support a karyotype of 1n = 30 chromosomes. Comparisons with other octopus species' genomes show a conserved octopus karyotype and a pattern of local genome rearrangements between species. This new chromosome-scale genome of O. vulgaris will further facilitate research in all aspects of cephalopod biology, including various forms of plasticity and the neural machinery underlying sophisticated cognition, as well as an understanding of cephalopod evolution.
Differential Longitudinal Associations Between Domains of Cognitive Function and Physical Function: A 20-Year Follow-Up Study
Authors: Sala Giovanni , Nishita Yukiko , Tange Chikako , Zhang Shu , Ando Fujiko , Shimokata Hiroshi , Otsuka Rei , Arai Hidenori 
Published: October 2023

Abstract

Objectives
Cognitive and physical functions are both associated with disability and death. Recent studies have addressed the relationship between cognitive declines and physical declines; however, whether various facets of cognition are diversely associated with specific physical functions is yet to be ascertained. The present work examines the longitudinal associations between fluid and crystallized cognitive functions (Gf and Gc) and physical functions.

Methods
The sample consisted of 863 community-dwelling older adults (baseline age 60–79 years) from the National Institute for Longevity Sciences-Longitudinal Study of Aging. The participants were tested on a set of Gf and Gc tests and physical tests (grip strength and gait speed). We ran a series of Multivariate Latent Growth Curve models. Specifically, we tested the relationship between cognitive and physical functions in terms of baseline performance (intercept) and rate of change (slope).

Results
The slope–slope correlations between Gf and physical function were large (grip strength r = 0.64 and gait speed r = 0.68, ps &lt; .001). By contrast, the slope correlations between Gc and physical functions were weak (rs ≤ 0.31) and barely or marginally significant (ps ≤ .06).

Discussion
The results show that distinct domains of cognitive functions have different associations with physical functions. Namely, the aging-associated declines in the tested physical functions are robustly correlated with the declines in Gf, but are only weakly correlated with the declines in Gc. Therefore, Gc measures may be poor proxies for the patient’s frailty and should be considered with caution in clinical assessment.
Pandemic and Older Adults’ Social Structure of Shared Time
Authors: Chen Jen-Hao 
Published: October 2023

Abstract

Objectives
Shared time with family and friends is crucial for older adults’ health and well-being. This study examines how a public health crisis affects older adults’ social connectedness through their shared time with known persons.

Methods
The study used data from the 2019–2020 American Time Use Survey (N = 9,697) to assess older adults’ (aged 50+) social structure of shared time before and during the coronavirus disease 2019 pandemic. Logistic regression and hurdle model with state-fixed effects were used to estimate the relationships between state-level pandemic severity and measures of older adults’ shared time while controlling for sociodemographic characteristics.

Results
There were small, statistically significant effects of pandemic severity on older adults’ shared time. State-level incidence rates and policy stringency indexes were correlated with a lower likelihood of, and a decline in, older adults’ shared time in public places and interactions with individuals outside their immediate family. State policy stringency was associated with more shared time in immediate family interactions. Furthermore, pandemic severity was not associated with a decrease in likelihood and older adults’ shared time with nonresident immediate family. Policy stringency was more consequential than the incidence rate for older adults’ social structure of shared time during the pandemic.

Discussion
Results indicate a place-based and role-based restructuring of older adults’ daily shared time during the pandemic. Despite small effect sizes, the substantial old age population implies significant changes in shared time and patterns of daily connection at the population level. Theoretical and policy implications of the findings are discussed.
metaGOflow: a workflow for the analysis of marine Genomic Observatories shotgun metagenomics data
Authors: Zafeiropoulos Haris , Beracochea Martin , Ninidakis Stelios , Exter Katrina , Potirakis Antonis , De Moro Gianluca , Richardson Lorna , Corre Erwan , Machado João , Pafilis Evangelos , Kotoulas Georgios , Santi Ioulia , Finn Robert D , Cox Cymon J , Pavloudi Christina 
Published: October 2023

Abstract

Background
Genomic Observatories (GOs) are sites of long-term scientific study that undertake regular assessments of the genomic biodiversity. The European Marine Omics Biodiversity Observation Network (EMO BON) is a network of GOs that conduct regular biological community samplings to generate environmental and metagenomic data of microbial communities from designated marine stations around Europe. The development of an effective workflow is essential for the analysis of the EMO BON metagenomic data in a timely and reproducible manner.

Findings
Based on the established MGnify resource, we developed metaGOflow. metaGOflow supports the fast inference of taxonomic profiles from GO-derived data based on ribosomal RNA genes and their functional annotation using the raw reads. Thanks to the Research Object Crate packaging, relevant metadata about the sample under study, and the details of the bioinformatics analysis it has been subjected to, are inherited to the data product while its modular implementation allows running the workflow partially. The analysis of 2 EMO BON samples and 1 Tara Oceans sample was performed as a use case.

Conclusions
metaGOflow is an efficient and robust workflow that scales to the needs of projects producing big metagenomic data such as EMO BON. It highlights how containerization technologies along with modern workflow languages and metadata package approaches can support the needs of researchers when dealing with ever-increasing volumes of biological data. Despite being initially oriented to address the needs of EMO BON, metaGOflow is a flexible and easy-to-use workflow that can be broadly used for one-sample-at-a-time analysis of shotgun metagenomics data.
‘An inferior technician’? African American signallers in the First World War
Authors: Hall Brian N 
Published: October 2023

Abstract
In the literature on the struggles of African Americans during the First World War, there has been a failure to examine the experiences of the 325th Field Signal Battalion, the first Black signal unit in the U.S. Army. Drawing upon a range of archival sources, unpublished letters, official documents and newspapers, this article assesses the experiences of the battalion’s Black officers and men before, during and after the war, arguing that they defied not only traditional notions of specialism within the army, threatening to destabilize unambiguous racial boundaries by challenging what had long been viewed as an intrinsically white soldiers’ sphere of influence, but also some of the fundamental principles underpinning Jim Crow ideology. In so doing, they made an important, albeit subtle, contribution to the ‘long civil rights movement’.
The contribution of the parliamentary press to Oliver Cromwell’s image as a military hero of the first English Civil War
Authors: Macadam Joyce 
Published: October 2023

Abstract
This article examines the parliamentary press’s role in promoting Oliver Cromwell’s heroic image during the first civil war, a topic that has received relatively little scholarly attention. Based on a detailed study of contemporary pamphlets and newsbooks, it suggests three phases of press coverage that correspond to the conflict’s campaigning seasons. It shows that Cromwell’s image in print was greatly assisted by his close relationship with the Independent leadership at Westminster, and their expertise in the manipulation of the press. For them, Cromwell’s successes in the field were essential for securing victory. His military reputation, initially nurtured by the civil war press, laid the foundations for his eventual role as lord protector.
Black American women’s attitudes toward seeking mental health services and use of mobile technology to support the management of anxiety
Authors: McCall Terika , Foster Meagan , Tomlin Holly R , Schwartz Todd A 
Published: October 2023

Abstract

Objectives
This study aimed to understand Black American women’s attitudes toward seeking mental health services and using mobile technology to receive support for managing anxiety.

Methods
A self-administered web-based questionnaire was launched in October 2019 and closed in January 2020. Women who identified as Black/African American were eligible to participate. The survey consisted of approximately 70 questions and covered topics such as, attitudes toward seeking professional psychological help, acceptability of using a mobile phone to receive mental health care, and screening for anxiety.

Results
The findings of the study (N = 395) showed that younger Black women were more likely to have greater severity of anxiety than their older counterparts. Respondents were most comfortable with the use of a voice call or video call to communicate with a professional to receive support to manage anxiety in comparison to text messaging or mobile app. Younger age, higher income, and greater scores for psychological openness and help-seeking propensity increased odds of indicating agreement with using mobile technology to communicate with a professional. Black women in the Southern region of the United States had twice the odds of agreeing to the use of mobile apps than women in the Midwest and Northeast regions.

Discussion
Black American women, in general, have favorable views toward the use of mobile technology to receive support to manage anxiety.

Conclusion
Preferences and cultural appropriateness of resources should be assessed on an individual basis to increase likelihood of adoption and engagement with digital mental health interventions for management of anxiety.
Determination of the optimal dietary zinc content for pigs between 10 and 30 kg body weight
Authors: Hansen Sally Veronika , Graffagnino Aurégane , Hedemann Mette Skou , Nielsen Tina Skau , Woyengo Tofuko Awori 
Published: October 2023

Abstract
The Zn requirement of pigs immediately after weaning is more investigated compared to the Zn requirement in the growth period between 10 and 30 kg. Unabsorbed and excessive dietary Zn is excreted mainly through feces, and spreading pig slurry to fields can cause environmental issues because high levels of Zn can impair plant growth and contribute to the development of antimicrobial resistance genes in microorganisms. Therefore, more precise knowledge of Zn requirements and dietary Zn recommendations is important. The present study investigated the optimal dietary Zn content for 10- to 30-kg pigs. The study used 150 pigs weaned at 28 d of age (day 0) and supplied with 1,474 mg dietary Zn/kg the first 2 wk post-weaning. After 2 wk, pigs were randomly distributed according to body weight (BW; 10.1 ± 0.3 kg) and sex, to individually housing, and fed a diet supplemented with either 0, 30, 60, 120, or 240 mg Zn/kg (from ZnO), resulting in total dietary Zn contents of 80, 92, 117, 189, and 318 mg/kg until week 6 post-weaning. BW, feed intake, and fecal scores were recorded, and samples of blood (weeks 2, 3, 5, and 6) and tissues (week 6) were collected. The feed intake, growth, feed efficiency, relative weight of the pancreas and liver, Zn concentration in the liver, and pancreatic digestive enzyme activity were unaffected by dietary Zn content (P &gt; 0.12). The serum Zn level decreased (P &lt; 0.01) by up to 24% from weeks 2 to 3. The serum Zn concentrations in weeks 5 and 6 were similar to in week 2 when 117, 189, and 318 mg Zn/kg were provided, while with 80 and 92 mg Zn/kg the serum Zn concentration was lower (P &lt; 0.01) than in week 2. The serum Zn concentration reached a plateau in weeks 5 and 6, and breakpoints were calculated at 126 ± 17 and 102 ± 6 mg Zn/kg, respectively. Bone Zn status was greater (P &lt; 0.01) with 189 than 80 mg Zn/kg and a breakpoint was calculated at 137 ± 19 mg Zn/kg. According to performance, the Zn requirement for 10- to 30-kg pigs can be fulfilled with 80 mg total Zn/kg, but based on serum and bone Zn status, the optimal total dietary Zn content is 102 to 137 mg/kg. The latter corresponds to a daily Zn intake (requirement) of 103 to 138 mg when calculated from the average feed intake during weeks 3 to 6 (1,005 g/d). Importantly, the presented results are obtained in pigs supplied with 1,474 mg Zn/kg from ZnO the first 2 wk post-weaning and a high level of phytase (1,000 phytase units) in the diet throughout the experiment.
Sand cat arithmetic optimization algorithm for global optimization engineering design problems
Authors: Chen Shuilin , Zheng Jianguo 
Published: October 2023

Abstract
Sand cat swarm optimization (SCSO) is a recently introduced popular swarm intelligence metaheuristic algorithm, which has two significant limitations – low convergence accuracy and the tendency to get stuck in local optima. To alleviate these issues, this paper proposes an improved SCSO based on the arithmetic optimization algorithm (AOA), the refracted opposition-based learning and crisscross strategy, called the sand cat arithmetic optimization algorithm (SC-AOA), which introduced AOA to balance the exploration and exploitation and reduce the possibility of falling into the local optimum, used crisscross strategy to enhance convergence accuracy. The effectiveness of SC-AOA is benchmarked on 10 benchmark functions, CEC 2014, CEC 2017, CEC 2022, and eight engineering problems. The results show that the SC-AOA has a competitive performance.
Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli—a rate limiting step in the induction of recombinant protein expression
Authors: Simas Rodrigo G , Pessoa Junior Adalberto , Long Paul F 
Published: October 2023

Abstract

Coupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This is because gene expression under lac operon regulation is tightly correlated with intracellular IPTG concentration due to allosteric regulation of the lac repressor protein (lacY). An in-silico mathematical model established that uptake of IPTG across the cytoplasmic membrane of E. coli by simple diffusion was negligible. Conversely, lacY mediated active transport was a rapid process, taking only some seconds for internal and external IPTG concentrations to equalize. Optimizing kcat and KM parameters by targeted mutation of the galactoside binding site in lacY could be a future strategy to improve the performance of recombinant protein expression. For example, if kcat were reduced whilst KM was increased, active transport of IPTG across the cytoplasmic membrane would be reduced, thereby lessening the metabolic burden on the cell and expediating accumulation of recombinant protein. The computational model described herein is made freely available and is amenable to optimize recombinant protein expression in other heterologous hosts.

One-Sentence Summary
A computational model made freely available to optimize recombinant protein expression in Escherichia coli other heterologous hosts.
High-grade appendiceal mucinous neoplasm presenting as renal colic; a case report and review of literature
Authors: Amir Baraa , Amir Amaar , Sheikh Salwa 
Published: October 2023

Abstract
High-grade appendiceal mucinous neoplasms (HAMN) are rare mucinous appendiceal tumors that are mostly incidentally discovered and histologically show marked cytoplasmic atypia. We report a 62-year-old female patient who was diagnosed with acute right flank pain mimicking renal colic. Abdominal and pelvis CT scans showed no calculi or hydronephrosis. Incidentally, a markedly distended retrocecal appendix was identified and an appendectomy was performed. Histopathology revealed an HAMN of size 5.8 cm. HAMN are one of the rare and somewhat recently described epithelial appendiceal tumors. The latest 2019 classification is based on histologic appearance and on the consensus for classification and pathologic reporting of Pseudomyxoma peritonei and associated appendiceal neoplasia (Peritoneal Surface Pathology Group International, modified Delphi process). Histologically, appendiceal mucinous lesions are classified as nonneoplastic/mucocele and neoplastic lesions with separate subcategorization. Despite its rarity, it is crucial for clinicians to be aware of HAMN to distinguish it from other clinical differentials.
Delayed presentation of isolated grade III pancreatic injury—a case report
Authors: Ferreira Maria João , Gallardo Gabriel , Vigia Emanuel , Filipe Edite , Marques Hugo Pinto 
Published: October 2023

Abstract
Because of their vague and subtle indications and symptoms, pancreatic injuries are frequently misdiagnosed. It’s crucial to have a high level of clinical suspicion. The presence of other organ solid lesions and vascular injuries, as well as the patient’s hemodynamic condition, will determine how these injuries are treated. A surgical approach is mandatory when a ductal disruption occurs. The case of a 32-year-old man who experienced an upper abdominal blunt trauma is presented. He was admitted to our hospital with an acute abdomen 48 hours later. A complete transection of the major pancreatic duct was discovered during surgical investigation, and a distal pancreatectomy with en bloc splenectomy was performed. Even in a delayed context, distal pancreatectomy can be safely performed and is the best option.