bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary molecular genetic analysis of the Receptor Interacting Protein 140 (RIP140) in women affected by endometriosis
Authors: Caballero Virginia , Ruiz Rocío , Sainz José Antonio , Cruz Marina , López-Nevot Miguel Angel , Galán José Jorge , Real Luis Miguel , de Castro Francisco , López-Villaverde Vicente , Ruiz Agustín 
Published: August 2005

Abstract

Background

Endometriosis is a complex disease affecting 10–15% of women at reproductive age. Very few genes are known to be altered in this pathology. RIP140 protein is an important cofactor of oestrogen receptor and many other nuclear receptors. Targeting disruption experiments of nrip1 gene in mice have demonstrated that nuclear receptor interacting protein 1 gene (nrip1), the gene encoding for rip140 protein, is essential for female fertility. Specifically, mice null for nrip1 gene are viable, but females are infertile because of complete failure of mature follicles to release oocytes at ovulation stage. The ovarian phenotype observed in mice devoid of rip140 closely resembles the luteinized unruptured follicle (LUF) syndrome that is observed in a high proportion of women affected of endometriosis or idiopathic infertility. Here we present a preliminary work that analyses the role of NRIP1 gene in humans.

Methods

We have sequenced the complete coding region of NRIP1 gene in 20 unrelated patients affected by endometriosis. We have performed genetic association studies by using the DNA variants identified during the sequencing process.

Results

We identified six DNA variants within the coding sequence of NRIP1 gene, and five of them generated amino acid changes in the protein. We observed that three of twenty sequenced patients have specific combinations of amino-acid variants within the RIP140 protein that are poorly represented in the control population (p = 0.006). Moreover, we found that Arg448Gly, a common polymorphism located within NRIP1 gene, is associated with endometriosis in a case-control study (59 cases and 141 controls, pallele positivity test = 0.027).

Conclusion

Our results suggest that NRIP1 gene variants, separately or in combinations, might act as predisposing factors for human endometriosis.
Why study the use of animal products in traditional medicines?
Authors: Alves Rômulo RN , Rosa Ierecê L 
Published: August 2005

Abstract
The World Health Organization (WHO) estimates that as many as 80% of the world's more than six billion people rely primarily on animal and plant-based medicines. The healing of human ailments by using therapeutics based on medicines obtained from animals or ultimately derived from them is known as zootherapy. The phenomenon of zootherapy is marked both by a broad geographical distribution and very deep historical origins. Despite their importance, studies on the therapeutic use of animals and animal parts have been neglected, when compared to plants. This paper discusses some related aspects of the use of animals or parts thereof as medicines, and their implications for ecology, culture (the traditional knowledge), economy, and public health.
Rheumatoid arthritis seropositive for the rheumatoid factor is linked to the protein tyrosine phosphatase nonreceptor 22-620W allele
Authors: Dieudé Philippe , Garnier Sophie , Michou Laëtitia , Petit-Teixeira Elisabeth , Glikmans Elodie , Pierlot Céline , Lasbleiz Sandra , Bardin Thomas , Prum Bernard , Cornélis François ,   the European Consortium on Rheumatoid Arthritis Families
Published: August 2005

AbstractThe protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene encodes for lymphoid tyrosine phosphatase LYP, involved in the negative regulation of early T-cell activation. An association has recently been reported between the PTPN22-620W functional allele and rheumatoid factor-positive (RF+) rheumatoid arthritis (RA), among other autoimmune diseases. Expected linkage proof for consistency cannot be definitely produced by an affected sib-pair (ASP) analysis. Our aim was therefore to search for linkage evidence with the transmission disequilibrium test.DNA from the French Caucasian population was available for two samples of 100 families with one RA patient and both parents, and for 88 RA index cases from RA ASP families. Genotyping was carried out by PCR-restriction fragment length polymorphism. The analysis was performed using the transmission disequilibrium test, genotype relative risk and ASP-based analysis.The transmission disequilibrium test of the PTPN22-620W allele revealed linkage and association for RF+ RA (61% of transmission, P = 0.037). The genotype relative risk showed the risk allele in 34% of RF+ RA patients and in 24% of controls derived from nontransmitted parental chromosomes (P = 0.047, odds ratio = 1.69, 95% confidence interval = 1.03–2.78). The ASP investigation showed no enriched risk allele in RA multiplex families, resulting in a lack of power of ASP analysis, explaining the published negative results.This study is the first to show linkage of PTPN22 to RF+ RA, consistent with PTPN22 as a new RA gene.
Relationship between the expression of cyclooxygenase 2 and MDR1/P-glycoprotein in invasive breast cancers and their prognostic significance
Authors: Surowiak Pawel , Materna Verena , Matkowski Rafal , Szczuraszek Katarzyna , Kornafel Jan , Wojnar Andrzej , Pudelko Marek , Dietel Manfred , Denkert Carsten , Zabel Maciej , Lage Hermann 
Published: August 2005

Abstract
Introduction
Recent reports suggest that expression of the cyclooxygenase 2 (COX-2) enzyme may up-regulate expression of MDR1/P-glycoprotein (MDR1/P-gp), an exponent of resistance to cytostatic drugs. The present study aimed at examining the relationship between the expression of COX-2 and of MDR1/P-gp in a group of breast cancer cases.

Methods
Immunohistochemical reactions were performed using monoclonal antibodies against COX-2 and MDR1/P-gp on samples originating from 104 cases of primary invasive breast cancer.

Results
COX-2-positive cases were shown to demonstrate higher expression of MDR1/P-gp (P < 0.0001). The studies also demonstrate that COX-2 expression was typical for cases of a higher grade (P = 0.01), a shorter overall survival time (P < 0.0001) and a shorter progression-free time (P < 0.0001). In the case of MDR1/P-gp, its higher expression characterised cases of a higher grade (P < 0001), with lymph node involvement (P < 0001), and shorter overall survival (P < 0.0001) and progression-free time (P < 0.0001).

Conclusion
Our studies confirmed the unfavourable prognostic significance of COX-2 and MDR1/P-gp. We also document a relationship between COX-2 and MDR1/P-gp, which suggests that COX-2 inhibitors should be investigated in trials as a treatment supplementary to chemotherapy of breast cancers.
Relation between respiratory variations in pulse oximetry plethysmographic waveform amplitude and arterial pulse pressure in ventilated patients
Authors: Cannesson Maxime , Besnard Cyril , Durand Pierre G , Bohé Julien , Jacques Didier 
Published: August 2005

Abstract
Introduction
Respiratory variation in arterial pulse pressure is a reliable predictor of fluid responsiveness in mechanically ventilated patients with circulatory failure. The main limitation of this method is that it requires an invasive arterial catheter. Both arterial and pulse oximetry plethysmographic waveforms depend on stroke volume. We conducted a prospective study to evaluate the relationship between respiratory variation in arterial pulse pressure and respiratory variation in pulse oximetry plethysmographic (POP) waveform amplitude.

Method
This prospective clinical investigation was conducted in 22 mechanically ventilated patients. Respiratory variation in arterial pulse pressure and respiratory variation in POP waveform amplitude were recorded simultaneously in a beat-to-beat evaluation, and were compared using a Spearman correlation test and a Bland–Altman analysis.

Results
There was a strong correlation (r2 = 0.83; P < 0.001) and a good agreement (bias = 0.8 ± 3.5%) between respiratory variation in arterial pulse pressure and respiratory variation in POP waveform amplitude. A respiratory variation in POP waveform amplitude value above 15% allowed discrimination between patients with respiratory variation in arterial pulse pressure above 13% and those with variation of 13% or less (positive predictive value 100%).

Conclusion
Respiratory variation in arterial pulse pressure above 13% can be accurately predicted by a respiratory variation in POP waveform amplitude above 15%. This index has potential applications in patients who are not instrumented with an intra-arterial catheter.
Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae
Authors: Eide David J , Clark Suzanne , Nair T Murlidharan , Gehl Mathias , Gribskov Michael , Guerinot Mary Lou , Harper Jeffrey F 
Published: August 2005

Abstract
Background
Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'.

Results
We identified 212 strains that showed altered ionome profiles when grown on a rich growth medium. Surprisingly few of these mutants (four strains) were affected for only one element. Rather, levels of multiple elements were altered in most mutants. It was also remarkable that only six genes previously shown to be involved in the uptake and utilization of minerals were identified here, indicating that homeostasis is robust under these replete conditions. Many mutants identified affected either mitochondrial or vacuolar function and these groups showed similar effects on the accumulation of many different elements. In addition, intriguing positive and negative correlations among different elements were observed. Finally, ionome profile data allowed us to correctly predict a function for a previously uncharacterized gene, YDR065W. We show that this gene is required for vacuolar acidification.

Conclusion
Our results indicate the power of ionomics to identify new aspects of mineral homeostasis and how these data can be used to develop hypotheses regarding the functions of previously uncharacterized genes.
E-Predict: a computational strategy for species identification based on observed DNA microarray hybridization patterns
Authors: Urisman Anatoly , Fischer Kael F , Chiu Charles Y , Kistler Amy L , Beck Shoshannah , Wang David , DeRisi Joseph L 
Published: August 2005

AbstractDNA microarrays may be used to identify microbial species present in environmental and clinical samples. However, automated tools for reliable species identification based on observed microarray hybridization patterns are lacking. We present an algorithm, E-Predict, for microarray-based species identification. E-Predict compares observed hybridization patterns with theoretical energy profiles representing different species. We demonstrate the application of the algorithm to viral detection in a set of clinical samples and discuss its relevance to other metagenomic applications.
Psychiatric Disorders and Mental Health Service Use Among Caregivers of Advanced Cancer Patients
Authors: Vanderwerker Lauren C. , Laff Rachel E. , Kadan-Lottick Nina S. , McColl Suzanne , Prigerson Holly G. 
Published: August 2005

Purpose Despite research demonstrating the psychological burden of caregiving for advanced cancer patients, limited information exists on the prevalence of psychiatric disorders and mental health service use among these informal caregivers. Methods Two hundred informal caregivers of advanced cancer patients were interviewed and administered the Structured Clinical Interview of the Diagnostic and Statistical Manual of Mental Disorders Fourth Edition and an assessment of mental health service use. Results Thirteen percent of caregivers met criteria for a psychiatric disorder; 25% accessed treatment for mental health concerns since the patient's cancer diagnosis. The frequencies of current psychiatric disorders were as follows: panic disorder, 8.0% (95% CI, 4.6% to 12.7%), major depressive disorder, 4.5% (95% CI, 2.1% to 8.4%), post-traumatic stress disorder, 4.0% (95% CI, 1.7% to 7.7%), and generalized anxiety disorder, 3.5% (95% CI, 1.4% to 7.1%). Among caregivers with a current psychiatric disorder, 81% discussed mental health concerns with a health professional before the patient's cancer diagnosis compared with 46% after the diagnosis (McNemar test = 5.40; P = .02). Only 46% of caregivers with a current psychiatric disorder accessed mental health services. Caregivers who discussed mental health concerns with a clinician before the patient's cancer diagnosis (odds ratio [OR] = 3.51; 95% CI, 1.42 to 8.71) and after the diagnosis (OR = 21.23; 95% CI, 9.02 to 49.94) were more likely than caregivers not having these discussions to receive mental health services. Conclusion Many caregivers of advanced cancer patients either meet criteria or are being treated for psychiatric problems. Discussing mental health issues positively influences the receipt of mental health services and should be actively pursued in this vulnerable population.
Quality-of-Life Effects of Prophylactic Salpingo-Oophorectomy Versus Gynecologic Screening Among Women at Increased Risk of Hereditary Ovarian Cancer
Authors: Madalinska Joanna B. , Hollenstein Judith , Bleiker Eveline , van Beurden Marc , Valdimarsdottir Heiddis B. , Massuger Leon F. , Gaarenstroom Katja N. , Mourits Marian J.E. , Verheijen René H.M. , van Dorst Eleonora B.L. , van der Putten Hans , van der Velden Ko , Boonstra Henk , Aaronson Neil K. 
Published: August 2005

Purpose Recommendations for women at high risk of ovarian cancer include periodic gynecologic screening (GS) and prophylactic bilateral salpingo-oophorectomy (PBSO). The aim of the current study was to determine the quality-of-life (QOL) effects of PBSO versus GS. Patients and Methods Questionnaire data were obtained from 846 high-risk women who had participated in this nationwide, cross-sectional, observational study. Forty-four percent of the women had undergone PBSO, and 56% had opted for GS. Topics addressed by the questionnaire included generic QOL, cancer-specific distress, endocrine symptoms, and sexual functioning. Results No statistically significant between-group differences were observed in generic QOL (Short Form-36), with women in both the PBSO and GS groups scoring similarly to the general population. Compared with GS, PBSO was associated with fewer breast and ovarian cancer worries (P < .001) and more favorable cancer risk perception (P < .05). However, the PBSO group reported significantly more endocrine symptoms (P < .001) and worse sexual functioning (P < .05) than the GS group. Eighty-six percent of women would choose PBSO again, and 63% would recommend it to a friend with familial risk of ovarian cancer. Conclusion PBSO had no measurable adverse impact on generic QOL of high-risk women. The favorable effects of PBSO in terms of reduced cancer worries and low perceived cancer risk need to be weighed against the increase in endocrine and sexual symptoms. Balanced information will help clinicians and high-risk women to make informed decisions about the optimal preventive health strategy.
Treatment Results in Localized Primary Gastric Lymphoma: Data of Patients Registered Within the German Multicenter Study (GIT NHL 02/96)
Authors: Koch Peter , Probst Andreas , Berdel Wolfgang E. , Willich Normann A. , Reinartz Gabriele , Brockmann Jens , Liersch Rüdiger , del Valle Francisco , Clasen Hermann , Hirt Carsten , Breitsprecher Regine , Schmits Rudolf , Freund Mathias , Fietkau Rainer , Ketterer Peter , Freitag Eva-Maria , Hinkelbein Margit , Heinecke Achim , Parwaresch Reza , Tiemann Markus 
Published: August 2005

Purpose In the prospective study 02/96 on primary GI lymphoma, we have collected data on histology, clinical features, and treatment results. In particular, in stages I and II localized primary gastric lymphoma (PGL), our objectives were to reduce treatment intensity and to confirm our hypothesis from study 01/92, which maintained that an organ-preserving approach is not inferior to primary surgery. Patients and Methods Patients receiving radiotherapy and/or chemotherapy were stratified for histologic grade, stage, and whether surgery had been carried out or not (as decided by each participating center). Patients with aggressive PGL received six cycles of CHOP-14 (cyclophosphamide, doxorubicin, vincristine, and prednisone) followed by involved-field radiotherapy (40 Gy). Patients with indolent PGL (including patients experiencing treatment failure with antibiotic therapy for Helicobacter pylori) were treated with extended-field radiotherapy. The volume depended on stage. The irradiation dose was 30 Gy, followed by a boost of 10 Gy (the latter omitted after complete resection) to the tumor region. Results Seven hundred forty-seven patients were accrued. Of these patients, 393 with localized PGL were treated with radiotherapy and/or chemotherapy only or additional surgery between December 1996 and December 2003. The survival rate at 42 months for patients treated with surgery was 86% compared with 91.0% for patients without surgery. Conclusion In this nonrandomized study (02/96), we reproduced the previous results of study 01/92 showing no disadvantage for an organ-preserving treatment. Therefore, primary stomach resection should be questioned.
Large Cleaved and Immunoblastic Lymphoma May Represent Two Distinct Clinicopathologic Entities Within the Group of Diffuse Large B-Cell Lymphomas
Authors: De Paepe Pascale , Achten Ruth , Verhoef Gregor , Wlodarska Iwona , Stul Michel , Vanhentenrijk Vera , Praet Marleen , De Wolf-Peeters Chris 
Published: August 2005

Purpose The reliability of immunohistochemistry for subdividing diffuse large B-cell lymphomas (DLBCL) into germinal center B-cell-like (GCB) and non-GCB prognostic subgroups is debated. In this study we evaluated the prognostic significance of such subgrouping on a series of 153 DLBCL patients. Furthermore, we investigated whether both subgroups could comprise clinicopathologic entities recognized by their morphology and characterized by a distinct phenotype, specific genetic abnormalities, and clinical characteristics. Patients and Methods All samples from patients were reviewed and morphologically subdivided into large cleaved, immunoblastic, and not otherwise specified DLBCL. GCB and non-GCB immunohistochemical profiles were established. The presence of chromosomal translocations involving BCL2, BCL6, and MYC and/or rearrangements of these genes was investigated. Results Subdividing DLBCL with either a GCB or non-GCB immunophenotypic profile was not of prognostic significance. Nevertheless, CD10 expression was a predictor of favorable outcome, whereas high bcl-2 expression and BCL6 rearrangement were adverse predictors of disease-free survival. Interestingly, large cleaved DLBCL was clearly associated with a GCB immunophenotypic profile, CD10 expression, BCL2 rearrangement, age younger than 60 years, and low to low/intermediate International Prognostic Index risk, but was not of prognostic significance. In contrast, immunoblastic morphology was associated with a non-GCB profile and was a significant predictor of unfavorable DFS. Conclusion Subdividing DLBCL into subgroups based on their immunohistochemical profile was not of prognostic significance. Nevertheless, it allowed the additional characterization of two lymphoma subgroups previously recognized in the Working Formulation. Both correspond to two distinct clinicopathologic entities within the DLBCL.
Characteristics of Water Adsorbed on TiO2 Photocatalytic Surfaces as Studied by 1H NMR Spectroscopy
Authors: Y. Nosaka Atsuko , Nosaka Yoshio 
Published: August 2005

Abstract
This account is a review of the research on the characteristics of adsorbed water molecules and the photocatalytic reaction sites of incorporated organic molecules on various TiO2 photocatalysts in powder and film forms by means of 1H NMR spectroscopy. The adsorbed water can be categorized into four distinctively different water species: i) rigid water species with restricted mobility near the solid surface; ii) less mobile water molecules in the intermediate water layer; iii) relatively mobile water molecules in the outer water layer; iv) very loosely adsorbed water molecules which exchange slowly with gaseous water molecules in air. The temperature dependence of the NMR signal of a small quantity of ethanol molecules incorporated in TiO2 suggests that the actual photocatalytic reaction site can be these adsorbed water regions. The surface conversion of hydrophilic properties of TiO2 films induced by photoirradiation is well reflected in the NMR signal change. The enhancement of water molecularly adsorbed on TiO2 films is evidently detected on UV irradiation; this phenomenon has been difficult to elucidate so far with alternative spectroscopic techniques.
External Quantum Efficiency and Electroluminescence Spectra of BIODE (Biomolecular Light-Emitting Diode) Fabricated from Horse-Heart Cytochrome c
Authors: Ikeda Shingo , Tajima Hiroyuki , Matsuda Masaki , Ando Yoriko , Akiyama Hidefumi 
Published: August 2005

Abstract
We fabricated organic light-emitting diodes (OLED) from horse-heart cytochrome c, and measured the electroluminescence (EL) spectra, current–voltage and EL intensity–voltage characteristics. The external quantum efficiency evaluated from those characteristics was 6–8 × 10−8 at an applied voltage of 13 V. The EL spectrum shows the Soret band around 400 nm, the Q band around 500 nm, and the 690 nm band. The three bands became obscure for a sample prepared in an acidic solution (pH = 1.68) and in a basic solution (pH = 10.01). Based on the energy diagram of the /ITO/cytochrome c/Al/ junction, we considered the difference in the EL spectrum from the photoluminescence or absorption spectrum.
Dioxygen Binding to a Cobalt(II) Porphycene Complex and Its Auto-Oxidized Cobalt(III) Complex
Authors: Shimakoshi Hisashi , Aritome Isao , Hirota Shun , Hisaeda Yoshio 
Published: August 2005

Abstract
The reaction between a cobalt porphycene complex, [CoII(TPrPc)] (TPrPc is a dianion of 2,7,12,17-tetrapropylporphycene), and dioxygen was investigated by means of UV–vis, ESR, and resonance Raman (RR) spectroscopies. These spectra indicate the formation of [CoIII–O2−•]. In the presence of pyridine as an axial ligand, the formation of a six-coordinated superoxide species was confirmed by its characteristic ESR spectrum. The six-coordinated dioxygen adduct exhibits an RR band at 1144 cm−1, which is characteristic of O–O stretching of superoxide. ν(Co–O2) stretching is also observed at 540 cm−1. The ν(O–O) and ν(Co–O2) wavenumbers in the complex were assigned by means of 18O2 substitution. ν(Co–O2) of the O2 adduct shows a higher frequency relative to those of cobalt porphyrin complexes. On standing for one day at room temperature, the compound was auto-oxidized to a CoIII complex, which was structurally determined by X-ray analysis. The complex crystallized in the monoclinic space group P21/n, a = 9.6266(4) Å, b = 12.6343(5) Å, c = 15.6566(6) Å, β = 99.1200(10)°, V = 1880.17(13) Å3, and Z = 2. The CoIII ion lies on the plane of the porphycenato core, and coordinates four porphycenato nitrogen atoms and two pyridine nitrogen atoms. The complex cation, [CoIII(TPrPc)(Py)2]+, exhibits a slightly distorted octahedral coordination geometry. The average Co–N (porphycenato pyrrole) distance and the Co–N (pyridine) distance are 1.929 and 1.952 Å, respectively.