bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural conservation and functional genetic adaptive evolution of chloroplast genomes in Cycle-cup Oaks
Authors: Li Yu , Zheng Si-Si , Wang Tian-Rui , Liu Mei-Hua , Kozlowski Gregor , Yi Li-Ta , Song Yi-Gang 
Published: December 2023

Abstract
Background: Cycle-cup oaks (Quercus section Cyclobalanopsis) are one of the principal components of forests in the tropical and subtropical climates of East and Southeast Asia. They have experienced relatively recent increases in the diversification rate, driven by changing climates and the Himalayan orogeny. However, the course, tempo, and mode of genome evolution in cycle-cup oaks remain largely unknown; in particular, how plant plastome genes and genomes evolve during rapid speciation. To address this question, we analyzed the complete plastomes of 50 species of Quercus section Cyclobalanopsis, 36 newly sequenced and 14 published species.
Results: Based on a series of analyses, including genome structure, genome annotation, GC content, repeat sequences, SSR components, codon usage bias, and comparative genomics, we found that Quercus section Cyclobalanopsishad a conserved plastome structure. Highly divergent regions, such as the ndhFand ycf1 gene regions and the petN—psbM and rpoB—trnC-GCA gene spacer regions, provided potential molecular markers for subsequent analysis. The maximum likelihood phylogenetic tree based on complete chloroplast genomes, coding DNA sequences, and highly divergent regions produced a poorly resolved genetic relationship of the plastid lineages of section Cyclobalanopsis. We identified nine protein-coding genes containing sites for positive selection: ndhA, ndhD, ndhF, ndhH, rbcL, rpl32, accD, ycf1, and ycf2.
Conclusions: We compared and analyzed the chloroplast genomes of 50 species of Quercus section Cyclobalanopsis to explore their diversity structure, phylogenetic relationships, and ecological adaptative evolution. These chloroplast genome data provide valuable information for deep insights into phylogenetic relationships and intraspecific diversity in Quercus.
Diabetic Epiretinal Membranes Unveiled: Exploring Staging and Optical Coherence Tomography-Based Classification for Precise Characterization
Authors: Güvenç Umay , Uney Guner , Unlu Nurten , Candan Özlem , Orman Gözde 
Published: December 2023

Abstract
Background/Objectives:To evaluate the applicability of an OCT-based staging system for diabetic epiretinal membranes (ERMs) and investigate the vascular and anatomical distinctions between idiopathic and diabetic ERMs.

Subjects/Methods:A total of 142 eyes of diabetic, idiopathic ERM patients and controls were retrospectively analysed. The OCT-based staging system by Govetto et al. was used for classifying ERMs into stages. Correlations were established between OCT-A parameters, visual acuity (VA), ERM stage, and structural findings on OCT.

Results:It was accepted p-value <0.05 as significant level on all statistical analysis.Ectopic inner foveal layer thickness (EIFL) showed no significant difference between ERM groups but positively correlated with stage severity in both. Positive correlation observed between the the stage and the central foveal thickness (CFT), outer and inner retinal layer (IRL) thickness in both groups. CFT exhibited positive correlations with Macular vessel density ratio (MVR) and negative correlations with foveal avascular zone (FAZ) in both groups.

Conclusion:OCT-based staging developed for idiopatic ERMs was found to be applicable to diabetic cases without retinopathy. Mechanical traction in idiopathic ERM primarily affects the IRL and retinal blood flow. The presence of significant correlations between retinal thickness and MVR strengthens our findings, as it highlights the meaningful relationship between tractional thickening and vascular distortion in both idiopathic and diabetic ERM cases.
Modification of Acetazolamide Synthesis: New Derivatives and Investigation of Their Biological Properties
Authors: falakshahi Maryam , Mahmoodi Nosrat , khalili behzad , Melal Sarah Poormoradkhan 
Published: December 2023

Abstract
Acetazolamide 1 is a carbonic anhydrase inhibitor used in the medical treatment of glaucoma, epileptic seizure, idiopathic intracranial hypertension, altitude sickness, etc. The synthesis of `acetazolamide from the corresponding thiol derivative 2 requires oxidation and converting it to the sulfonyl chloride intermediate 3.
This research tries to improve the safety, green conditions and efficiency of the oxidation method. For this purpose, chlorine gas was replaced with sodium hypochlorite (commercial bleach). This procedure led to the synthesis of several new products 1a-f with high purity. The synthesized key sulfonyl chloride 3 in the reaction with various amines, hydrazones, and bis-amines led to several new sulfonamide derivatives. The antibacterial activity (AA) and antioxidant activity (AO) of the newly synthesized compounds was investigated. The AA effect of bis-products (4a and 4b) is significantly increased compared to the corresponding mono-product (1a). In addition, the synthesized hydrazine-based products (10a-10 g) showed a very good effect on all Gram-positive and Gram-negative bacteria compared to other products, especially (11 g), compared to ciprofloxacin and tetracycline. Compounds 11c, 11a, 11b, 11e, and 1d showed good AO compared to ascorbic acid.
Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95
Authors: Xianwei Yang , Tao Wang , Wentao Wang , Yin Chen 
Published: December 2023

Abstract
Objective
The echinococcosis-specific antigens Eg95 and AgB8/1 and the Em18 gene sequences were fused and expressed as the novel recombinant antigens rAgB8/1-Em18-Eg95 (T3) and rEm18-Eg95 (T2), used for the diagnosis of hydatid disease, prepared into an enzyme-linked immunosorbent reaction (ELISA) kit, and evaluated for their serological diagnostic value.
Method
The genes sequences were obtained from the GenBank database, and the T3 and T2 gene sequences were optimized and transformed into the pET-22b (+) BL21(DE3) prokaryotic expression system. Expression was induced, and the bacteria were disrupted. After obtaining the target recombinant protein, an ELISA was used to evaluate the serological diagnostic effect of the recombinant antigens.
Results
T3 and T2 recombinant gene sequences were successfully constructed. The relative molecular weight of the T3 protein was 88.1 kDa. The prokaryotic expression of T3 was higher in the supernatant, which was consistent with the predicted molecular weight. The purified concentration was 1.5 mg/mL, and the purity was 80%. The relative molecular weight of T2 protein was 79.9 kDa, the total protein concentration was 0.5 mg/mL, and the purity was less than 50%. The overall coincidence rate of T2 protein was low, and it was impossible to distinguish between negative and positive sera. The T3 antigen was coated at 1.0 µg/mL, the cutoff value was 0.5271, and the serum dilution ratio was 1:400. A T3 ELISA 96T kit was constructed to detect the serum of 272 clinically and pathologically confirmed cases. The sensitivity of T3 was 93.75%, and the specificity was 93.75%. The Youden coefficient was 77.08%, the parasite cross-reaction was 30%. Satisfactorily, the Pearson correlation coefficient between the T3 OD value and lesion diameter was 0.707, showing a strong correlation.
Conclusions
T3 exhibits good antigenicity, and the prepared ELISA diagnostic kit reached the laboratory diagnostic level of commercial kits. T3 can distinguish human cystic echinococcosis (CE) and alveolar echinococcosis (AE) more significantly and predict the diameter of lesions according to the OD value, which provides practical value for drug or surgical efficacy.
Probabilistic Physics-integrated Neural Differentiable Modeling for Isothermal Chemical Vapor Infiltration Process
Authors: Wang Jian-Xun , Akhare Deepak , Chen Zeping , Gulotty Richard , Luo Tengfei 
Published: December 2023

Abstract

Chemical vapor infiltration (CVI) is a widely adopted manufacturing technique used in producing carbon-carbon and carbon-silicon carbide composites. These materials are especially valued in the aerospace and automotive industries for their robust strength and lightweight characteristics. The densification process during CVI critically influences the final performance, quality, and consistency of these composite materials. Experimentally optimizing the CVI processes is challenging due to long experimental time and large optimization space. To address these challenges, this work takes a modeling-centric approach. Due to the complexities and limited experimental data of the isothermal CVI densification process, we have developed a data-driven predictive model using the physics-integrated neural differentiable (PiNDiff) modeling framework. An uncertainty quantification feature has been embedded within the PiNDiff method, bolstering the model's reliability and robustness. Through comprehensive numerical experiments involving both synthetic and real-world manufacturing data, the proposed method showcases its capability in modeling densification during the CVI process. This research highlights the potential of the PiNDiff framework as an instrumental tool for advancing our understanding, simulation, and optimization of the CVI manufacturing process, particularly when faced with sparse data and an incomplete description of the underlying physics.
Characterization of acid-mechanical milling pretreated rice straw for subcritical water hydrolysis
Authors: Yang Wei , Yang Fan , Wu Shengji , Che Lei 
Published: December 2023

Abstract

Ball milling of rice straw impregnated with sulfuric acid (RS-S), hydrochloric acid (RS-H), acetic acid (RS-A), or nitric acid (RS-N) were carried out in this study. Physicochemical analysis and subcritical water hydrolysis were performed to evaluate the effect of acid species on ball milling treatment of rice straw. Acetic acid and solo ball milling treatment showed little effect on solubility, thermal stability and crystalline structure of rice straw, while hydrochloric acid, acetic acid and nitric acid significantly improved the solubility and decreased the crystallinity index and thermal stability of rice straw. Sulfuric acid was found to be the most efficient acid to destroy the rice straw structure during ball milling followed by nitric acid and hydrochloric acid, attributed to its long retention on rice straw surface after drying. The effective cleavage of holocellulose-lignin chemical linkages in RS-S during pretreatment made the hydrolysis products of RS-S easier to be hydrolyzed to biochar at high reaction temperatures, resulting in the increased solid residue yield. The breakage of crystallites and holocellulose-lignin chemical linkages greatly improved the reactivity of RS-S, resulting in the lower temperature and activation energy required to initiate the hydrolysis reaction compared with those of rice straw and RS-A.
Fractional-order effect on soliton solution and the oscillation number for some time-space fractional higher-order nonlinear Schr\"odinger equations
Authors: Zhao Xinyu , Li li , Yu FaJun 
Published: December 2023

Abstract
The time(T)- and time-space(TS)-fractional higher-order Schro ̈dinger(FHNLS) equations are stud- ied, which can describe the dispersion and nonlinearity of optical pulse propagating in nonuniform fiber systems. The generalized fractional wave transformation is appropriately used to convert the T- and TS-FHNLS equations into some ordinary differential equations, and the real and imaginary parts are separated respectively. A few of new exact analytical solutions with fractional parameters α and β are obtained with the homogeneous balance method, including time-fractional and time-space frac- tional bright and dark solutions, and some fractional order effects on soliton solutions are considered. Some dynamic behaviors of the hyperbolic soliton solutions of the fractional-typed sech and tanh are obtained, some novel forms of the dark solitary wave and bright solitary wave are presented with the parameters of α and β. And some novel toroidal, spherical bright and dark solitons are derived by the toroidal and spherical coordinate transformations. Further, the four kinds of time-space fractional non-autonomous soliton solutions of the higher-order Gross-Pitaevskii(GP) model are derived, we find that the value of α decreases, the number of oscillations or singularities increases for small time values and decreases for large time values. These results are significantly different from the classical higher-order nonlinear Schro ̈dinger and GP equations, which helps to understand the solitary wave transmission pattern and applies to the physical manipulation.
The process of student engagement in school health promotion: A scoping review
Authors: Kontak Julia , Macrae Caitlyn , Caldwell Hilary , Feicht Becky , Heath Stephanie , Friesen Camille Hancock , Kirk Sara 
Published: December 2023

Abstract
Background
Health Promoting Schools (HPS) is a whole school model that strengthens and maintains a healthy school environment. While a key component of HPS is the engagement of students, there is little known about the strategies for, facilitators of, and barriers to, student engagement. The purpose of this scoping review was to summarize and characterize the evidence on the process of student engagement in school health promotion, with a focus on whole school models like HPS.
Methods
This scoping review followed the Joanna Briggs Institute guidelines and the Arksey and O’Malley scoping review framework. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews guided reporting. Eligibility included sources examining the process of student engagement in school health promotion for children and youth (ages 5 – 19) in any country, who attended a private or public school. Both published and unpublished sources were included. Databases searched were: CINAHL, ERIC, MEDLINE, Scopus, and Google Scholar. Relevant organisational websites and sources identified by experts were also reviewed. Two independent reviewers screened the title, abstract, and full text of the sources. Descriptive analysis was conducted for quantitative data, and content analysis was employed for qualitative data.
Results
1740 sources were screened, 133 citations were eligible for full text review and a total of 50 sources were included: 38 peer-reviewed publications, 7 grey literature sources, 2 peer-reviewed publications from reference-checking and 3 sources recommended by experts. The majority of articles reporting on primary research (n = 34) employed qualitative methods (n = 28) and over half of all sources were published from European institutions/organizations (n = 26). Process strategies for student engagement predominantly related to participatory mechanisms including reflection and visioning, determining priorities and action-oriented learning. A wide range of intersecting facilitators and barriers were identified, with school structures largely acting as a barrier and adult approaches to engagement being a facilitator.
Conclusion
This scoping review described the strategies, facilitators and barriers involving the process of student engagement in school health promotion. The results can inform the development and implementation of future student engagement strategies to strengthen school health promotion actions.
Delay of diagnosis of tumor in children and influence on prognosis. Experience of the AIEOP Centre of Bologna.
Authors: Rondelli Roberto , Prete Arcangelo , Belotti Tamara , Masetti Riccardo , Morigi Francesca , Riolo Serena , Battista Antonia Di , Ronchini Laura , Baccelli Francesco , Gottardi Francesca , Fois Maura , Grasso Antonio , Venturelli Francesco , Mercolini Federico , Legnani Elena Lara , Cantarini Maria Elena , Melchionda Fraia , Facchini Elena , Pession Andrea 
Published: December 2023

Abstract
Background The Pediatric Oncology and Hematology Unit Centre of Bologna adhere to the Italian Pediatric Hematology and Oncology Association (AIEOP) that since 1989 register any child diagnosed and treated with a malignant tumor, with the aim to quantify the number of cases diagnosed and treated, enter or not to official diagnostic-therapeutic protocols and the extraregional migration in the different Italian centres.Methods Data collection is performed adopting an ad-hoc form named Model 1.01 (Mod.1.01) through an electronic database open in the net to current 47 AIEOP Centres recruits annually about 1400 children (age 0–14 years) and 200 adolescents (age 15–19 years).Results From 1989 to 2018, out of 44730 AIEOP patients < 20 years at diagnosis, 1734 (3.9%) were accrued from Bologna Centre, 1528/1734 (88.1%) resulted resident in Italy and 877/1734 (50.6%) evaluable for this analysis. Most cases were ALL (343, 39.1%), followed by neuroblastoma (126, 14.4%), NHL (98, 11.2%), HL (66, 7.5%), ANLL (64, 7.3%), WT (56, 6.4%) and other types (124, 14.1%).Conclusions In order to evaluate the impact of a delay of diagnosis on outcome, with a mean of 4 days of latency between arrival to the center and diagnosis, OS for patients with < 4 days delay was significantly better than for patients with ≥ 4 days delay (p = 0.0236), resulting furthermore a prognostic factor such as age, diagnosis type and period of diagnosis in a multivariate fashion.
Intervening effect of polysaccharides on structured soy-based meat analogues by high- moisture extrusion
Authors: Zhao Yu , Sui Wenjie , Zhang Jingxin , Liu Rui , Wu Tao , Xu Jinchuan , Zhang Min 
Published: December 2023

Abstract
This study investigates the impact of four polysaccharides on enzyme-modified soybean protein concentrate (ESPC) within a high-water extrusion environment. Optimal texture and fibrous structure were achieved with 1% konjac glucomannan (KGM), 2% carrageenan (CA), 3% sodium alginate (SA), and 6% wheat starch (WS), respectively. These findings align with both macroscopic and microscopic observations. Structural analyses revealed that the presence of polysaccharides facilitated the formation of β-sheet structures, increased S-S bond content, and enhanced the ordered structure of ESPC. Water distribution and thermal property tests indicated that these polysaccharides improved water-holding capacity and mitigated the degradation rate of ESPC. In vitro digestion experiments demonstrated a 9.3% decrease in the digestibility of ESPC-CA compared to ESPC. This reduction could be attributed to electrostatic attraction between the protein and polysaccharide, creating a physical barrier that hindered the digestive process. This study provides insights into the influence of four polysaccharides on ESPC within a high-moisture extrusion environment. The information derived from this research holds potential significance for the development of high-quality plant-based meat analogs.
High performance Sodium Storage of Nano-CoSe 2 at Nitrogen Doping C arbon Frameworks with Carbon Nanotube Shuttles
Authors: An Ruifeng , Wang Bolun , Niu Hongkun , Liu Zhiwei , Gao Bingliang 
Published: December 2023

Abstract
CoSe2 exhibits a high theoretical sodium storage capacity as an anode material for sodium-ion batteries (SIBs). However, it faces several challenges during electrochemical cycling, including the shuttle effect of sodium polyselenide, volume expansion, and poor electrical conductivity. To address these issues, this study proposes a novel approach to synthesize CoSe2@NC/CNTs materials using cross-linked PMMA spheres as templates. The resulting structure demonstrates a hollow core-shell configuration comprising nitrogen-doped carbon nanotubes (N-CNTs) and dispersed CoSe2 nanoparticles. This was confirmed through characterization techniques such as XRD, RAMAN, SEM, TEM, etc., which verified the successful preparation. Moreover, XPS and TG analyses revealed the presence of strong interfacial interactions within the structure. These unique structural features enable CoSe2@NC/CNTs to exhibit exceptional sodium storage performance: remarkable stability (after 5000 cycles at 20 A·g− 1, the specific capacity only slightly decreases from 320 mAh·g− 1 to 280 mAh·g− 1 with a minimal degradation rate of 0.8% per cycle); and practicality (the specific capacity of the full battery NTO||CoSe2@NC/CNTs assembled with sodium nickel-iron-manganate (NTO) remains at 323 mAh·g− 1 after 120 cycles at a high current intensity of 5 A·g− 1). Additionally, we elucidate the intrinsic reason behind the strong stability of CoSe2@NC/CNTs through SEM and EDS analysis after cycling while also providing EX-situ characterization to explain their sodium storage.
Characterization of Anopheles gambiae breeding sites and insecticide resistance mechanisms in urban areas of Cotonou and Natitingou, Benin
Authors: Djègbè Innocent , HESSOU-DJOSSOU Donald , Boukari Massioudou Koto Yerima Gounou , Nonfodji Odilon , Tchigossou Geneviève , Djouaka Rousseau , Cornelie Sylvie , Akogbeto Martin , Djogbenou Luc , Chandre Fabrice 
Published: December 2023

Abstract
Background
This study aimed to investigate the relationship between the physicochemical characteristics of An. gambiae and An. Coluzzii breeding sites, the susceptibility to common insecticides used in public health, and the underlying insecticide resistance mechanisms.
Methods
Anopheles breeding sites surveys were conducted in Cotonou and Natitingou. Physicochemical parameters and bacterial loads were determined in individual breeding sites. The WHO susceptibility assays were carried out using the female of the emerging adult mosquitoes. Anopheles species were identified through PCR techniques. Kdr L1014F/S, N1575Y and G119S mutations were investigated using TaqMan genotyping assays.
Results
Molecular analysis showed that all mosquitoes analyzed in Cotonou were Anopheles coluzzii, while those of Natitingou were Anopheles gambiae s.s. WHO susceptibility assay indicated a high level of resistance to deltamethrin in the two cities. The resistance levels to deltamethrin were higher in Cotonou (X2 = 31.689; DF = 1; P < 0.0001). There was a suspicion of resistance to bendiocarb in Cotonou, whereas the mosquito population in Natitingou was resistant. The kdr L1014F mutation was highly observed in both mosquito populations, while the Ace-1 mutation was found in a small proportion of mosquitoes. In Cotonou, salinity was the only recorded physicochemical parameter significantly correlated with the resistance of Anopheles mosquitoes to deltamethrin. In Natitingou, significant correlations were observed between the allelic frequencies of the kdr L1014F mutation and pH, conductivity, and TDS.
Conclusion
These results indicate a high level of pyrethroid resistance in the An. gambiae population of both Cotonou and Natitingou. Moreover, the data suggest the involvement of the kdr mutation and possibly the breeding sites abiotic factors.