bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of different hydrolysis methods on composition and functional properties of fish protein hydrolysate obtained from Bigeye tuna waste
Authors: Abd El‐Rady Tasneem K. , Tahoun Al‐Azab M. , Abdin Mohamed , Amin Hesham F. 
Published: October 2023

SummaryThe objective of this research is to investigate the impact of different hydrolysis (chemical, enzymatic and microbiological) methods of Bigeye tuna waste (Thunnus obesus) on yield, degree of hydrolysis (DH), chemical composition, amino acid profile, functional properties and microbial quality of FPH produced. The enzymatic technique produced the highest yield (11.04%) and the greatest DH (39.04%) while it gave the lowest average peptide chain length (2.56) (P < 0.05). Also, protein and fat (76.37% and 10.82%, respectively) were the highest in the enzymatic hydrolyzed fish (FPH‐E) followed by microbial fermentation (FPH‐M) (P < 0.05). In FPH‐E, FPH‐M and chemical fish protein hydrolysates (FPH‐C), the essential to non‐essential amino acid ratios were 2.43, 1.79 and 0.51, respectively. They also had good functional properties, although they were less than FPH‐C in terms of water binding capacity (3.47 and 4.18 g g−1), oil binding capacity (9.84 and 7.38 g g−1) and foaming capacity (25% and 30%, respectively), and their foam stability rate was high. All FPHs products had a low range of microbial counts, with the FPH‐M exhibiting the greatest total count of bacteria, lactic acid bacteria and moulds and yeasts. Therefore, the biologically extracted FPH especially FPH‐E has the best compositions and qualities, making them an alternative ingredient for fish meal in fish feed formulation.
Association of the Arabidopsis oleoyl Δ12‐desaturase FAD2 with pre‐cis‐Golgi stacks at endoplasmic reticulum‐Golgi‐exit sites
Authors: Launhardt Larissa , Uhlenberg Johanna , Stellmach Hagen , Schomburg Marie , Hause Bettina , Heilmann Ingo , Heilmann Mareike 
Published: October 2023

SUMMARYThe unsaturation of phospholipids influences the function of membranes. In Arabidopsis thaliana, the oleoyl Δ12‐desaturase FAD2 converts oleic (18:1Δ9) to linoleic acid (18:2Δ9,12) and influences phospholipid unsaturation in different cellular membranes. Despite its importance, the precise localization of Arabidopsis FAD2 has not been unambiguously described. As FAD2 is thought to modify phospholipid‐associated fatty acids at the endoplasmic reticulum (ER), from where unsaturates are distributed to other cellular sites, we hypothesized that FAD2 locates to ER subdomains enabling trafficking of lipid intermediates through the secretory pathway. Fluorescent FAD2 fusions used to test this hypothesis were first assessed for functionality by heterologous expression in yeast (Saccharomyces cerevisiae), and in planta by Arabidopsis fad2 mutant rescue upon ectopic expression from an intrinsic FAD2 promoter fragment. Light sheet fluorescence, laser scanning confocal or spinning disc microscopy of roots, leaves, or mesophyll protoplasts showed the functional fluorescence‐tagged FAD2 variants in flattened donut‐shaped structures of ~0.5–1 μm diameter, in a pattern not resembling mere ER association. High‐resolution imaging of coexpressed organellar markers showed fluorescence‐tagged FAD2 in a ring‐shaped pattern surrounding ER‐proximal Golgi particles, colocalizing with pre‐cis‐Golgi markers. This localization required the unusual C‐terminal retention signal of FAD2, and deletion or substitutions in this protein region resulted in relaxed distribution and diffuse association with the ER. The distinct association of FAD2 with pre‐cis‐Golgi stacks in Arabidopsis root and leaf tissue is consistent with a contribution of FAD2 to membrane lipid homeostasis through the secretory pathway, as verified by an increased plasma membrane liquid phase order in the fad2 mutant.
Racial/Ethnic Differences in Physical Activity in a Low-Income Sample in Texas
Authors: Ranjit Nalini , Badillo David J. , Hoelscher Deanna M. , Macias Sarah , Gonzalez Alejandra , Wilkinson Anna V. 
Published: October 2023

Introduction: National data reveal that the age-adjusted prevalence of leisure-time physical inactivity is higher among Blacks and Hispanics compared with Whites. However, these estimates do not consider nonleisure physical activity (PA). Also, race/ethnicity in these findings may by confounded by socioeconomic status disparities in PA. Here, we examine racial/ethnic differences in multiple measures of PA within a lower socioeconomic status sample. Methods: Participants in the current cross-sectional study (n = 1526 adults, aged ≥ 18 y) were recruited from Supplemental Nutrition Assistance Program-Education classes (nutrition education classes that target low-income people) in Texas. Self-report data were obtained using survey questionnaires in spring and fall 2018. PA outcomes of 4 different intensities were assessed: mean daily time spent walking, engaging in moderate and vigorous PA, and sitting. Additional PA-related measures included use and awareness of community PA resources. Linear regression models examined racial/ethnic differences in the 4 PA outcomes after adjusting for participant gender, age, household composition measures, and various socioeconomic status measures. Results: In this low-income sample, Hispanic and Black participants spent 6 to 9 more minutes per day walking and engaging in moderate and vigorous PA compared with White/other participants (P < .05 for each measure). Conversely, White/other participants reported spending 82 more minutes sitting per day than Black and Hispanic participants (P < .01). Overall, Black participants were most likely to utilize community PA resources and report ease of engaging in exercise. Discussion: Together, these results reveal greater engagement in PA by racial/ethnic minorities in low-income communities compared with Whites. Our results have implications for tailoring PA programming to these communities.
Wünderlich’s syndrome in a haemodialysis patient
Authors: Wood Anna , Ismail Ibrahim 
Published: October 2023

Wünderlich’s syndrome, or spontaneous renal haemorrhages, are rare, atraumatic, intraparenchymal and perirenal haemorrhages, which are difficult to diagnose and can be potentially fatal.Patients who are dialysis-dependent are at an increased risk of bleeding, due to an association between uraemia and platelet dysfunction; for this reason, the use of double antiplatelets is avoided in this cohort. Case studies exist demonstrating spontaneous bleeds in these patients.Fish oil is used increasingly within medicine: however, it is known to interfere with platelet aggregation, therefore, theoretically increasing the tendency to bleed. The topic remains under debate, with systemic reviews refuting a correlation between intraoperative and postoperative bleeding and fish oil consumption. There is, however, an absence of literature on the adverse effects of fish oil when taken in large quantities.This case study explores the case of a patient who had a spontaneous renal bleed following a large, self-medicated dose of fish oil.
Assessment of the Safety and Efficiency of a Preperitoneal Continuous Infusion Using Bupivacaine after Abdominal Laparotomy in Digestive Carcinology
Authors: Ben-Saghroune Hayat , Abdessadek Mohammed , Achour Sanae , Kfal Youssef , El Bouazzaoui Abderrahim , Kanjaa Nabil , Sbai Hicham 
Published: October 2023

The purpose of this paper is to evaluate the safety and efficacy of continuous preperitoneal wound infiltration using bupivacaine after abdominal laparotomy in relation to plasma bupivacaine concentration and visual analog scale. Our study was performed on 60 adult patients with digestive cancer, operated at laparotomy, and randomized into two groups: bupivacaine and saline groups. The wound infiltration was through a multiperforated catheter along the scar. For the bupivacaine group, 0.25% bupivacaine was used; however, for the saline group, only saline (0.9%) was infiltrated. The pain was assessed by using the visual analog scale (VAS) in both groups. Plasma bupivacaine concentration was measured by high-performance liquid chromatography. The bupivacaine group had significantly lower postoperative morphine consumption and lower postoperative pain than the saline group (

P

< 0.0001). The majority of patients in the bupivacaine group had significant relief with the VAS scores of less than 3/10 cm at rest and 6/10 cm on mobilization. However, for the saline group, the VAS scores were higher than 6/10 cm either at rest or with mobilization. There was no clinical sign of toxicity and no technical complications for the bupivacaine group. Only eleven patients required morphine in this group, but the majority of patients received morphine at different doses in the saline group. Plasma bupivacaine was at very low concentrations. Overall, the current study has confirmed that continuous preperitoneal wound infiltration as postoperative analgesia is a simple, effective, and safe technique. It allows decreasing of morphine consumption and subsequently canceling their side effects.
ДОСЛІДЖЕННЯ ПРОСТАТОПРОТЕКТОРНОЇ ДІЇ ГУСТОГО ЕКСТРАКТУ З ГРИБІВ МАЙТАКЕ НА МОДЕЛІ ТЕСТОСТЕРОН-ІНДУКОВАНОЇ ГІПЕРПЛАЗІЇ ПЕРЕДМІХУРОВОЇ ЗАЛОЗИ
Authors: Герасимець І. І. 
Published: October 2023

РЕЗЮМЕ. Аденома передміхурової залози посідає перше місце серед чоловічих урологічних захворювань. Вона призводить до значного погіршення якості життя чоловіків унаслідок порушень сечостатевої системи та репродуктивної функції, порушується і психоемоційний стан. Для лікування простатиту застосовують різноманітні терапевтичні заходи, проте ефективність лікування залишається на низькому рівні. Тому пошук та створення нових препаратів з групи простатопротекторів є актуальним.
Метою роботи було експериментальне вивчення простатопротекторних властивостей густого екстракту з грибів майтаке за умов тестостерон-індукованої доброякісної гіперплазії передміхурової залози у білих щурів.
Матеріал і методи. Аденому простати моделювали шляхом підшкірного введення розчину тестостерону пропіонату в дозі 3 мг/кг білим щурам-самцям протягом 21 доби. Густий екстракт грибів майтаке піддослідним тваринам вводили внутрішньошлунково щоденно в дозі 150 мг/кг маси тіла щура одночасно з підшкірним введенням тестостерону пропіонату протягом 3 тижнів. Препарат порівняння “Простатофіт” в розведенні 1:10 у дозі 1 мл/100 г маси тіла тварини вводили внутрішньошлунково за такою ж схемою, що й екстракт грибів майтаке. Для проведення лабораторних досліджень брали гомогенат простати та сироватку крові щурів. Простатопротекторну дію екстракту з грибів майтаке оцінювали за активністю кислої та лужної фосфатази, вмістом ТБК-активних продуктів та відновленого глутатіону, розраховували фосфатазний індекс (КФ/ЛФ).
Результати. Введення тестостерону пропіонату білим щурам-самцям протягом 21 доби вірогідно порушує активність фосфатаз, призводить до підвищення ТБК-активних продуктів та зниження вмісту відновленого глутатіону в сироватці крові та гомогенаті простати уражених тварин. Доведено ефективність застосування густого екстракту з грибів майтаке за умов тестостерон-індукованої доброякісної гіперплазії передміхурової залози у щурів. Досліджуваний екстракт пригнічує перекисне окиснення ліпідів та нормалізує антиоксидантно/прооксидантний баланс в організмі тварин з патологією.
Висновки. Густий екстракт з грибів майтаке за умов модельованої гіперплазії передміхурової залози достовірно проявляє простатопротекторну дію. Отримані результати досліджень свідчать про антиоксидантну дію досліджуваного екстракту, оскільки гриби майтаке містять у своєму складі стероїдні сполуки, феноли, флавоноїди та вітаміни.
СИНДРОМАЛЬНА ХАРАКТЕРИСТИКА ДИТЯЧОГО ТРАВМАТИЗМУ
Authors: Процайло М. Д. , Процайло О. М. , Немченко К. Ю. 
Published: October 2023

РЕЗЮМЕ. Мета – вивчити основні синдромальні характеристики дитячого травматизму. Детально описати маловідомі раніше закономірності каліцтв у дітей – синдром травматологічної дивергенції, синдром травматологічної тріади, портрет дитини, уразливої до травм. Знання цих синдромів допоможе в практичній діяльності медичних та соціальних працівників значно знизити частоту пошкоджень у дітей.
Для реалізації цієї мети було поставлено такі завдання: дослідити основну сезонну закономірність травматизму; дослідити основну закономірність пошкодження топографо-анатомічних зон дитини; дати загальну характеристику дитини, схильної до травм; розробити основні напрямки профілактики дитячого травматизму.
Матеріал і методи. Здійснювався щомісячний моніторинг показників дитячого травматизму протягом 2020 року в абсолютних цифрах. Аналізували випадки травм у дітей, яким була надана допомога в обласному дитячому травмпункті. Обстежено 7 434 дитини. Пошкодження були об’єднані в загальні групи – переломи верхніх та нижніх кінцівок, вивихи, тупі травми, розтягнення зв’язок суглобів, рани голови, верхніх та нижніх кінцівок. Вивчалася частота пошкоджень кожної топографо-анатомічної ділянки у дітей – голова, кінцівки, кістки таза, хребет.
Травми, які мали спільну причину, виокремили у групи травматизму – побутовий, вуличний, шкільний, спортивний, ДТП.
Вивчали математичну залежність показників травматизму відносно пори року, оточуючого середовища. Отримані показники відображали в абсолютних цифрах у відсотках. Для більш наочної демонстрації цих закономірностей використовували графіки, таблиці.
Результати. Понад 90 % випадків травматизму припадає на вуличний – 5873 (79,9 %), побутовий – 398 (19,0 %). Виявлено характерну сезонну динаміку побутового та вуличного травматизму – синдром травматологічної дивергенції. Максимальні показники травматизму припадають на три літні місяці – синдром травматологічної тріади. Домінували переломи кінцівок. Виявлено основні риси характеру дітей схильних до травм – портрет дитини, уразливої до травм.
Transcriptomic analysis identifies CYP27A1 as a diagnostic marker for the prognosis and immunity in lung adenocarcinoma
Authors: Yin Yi , He Muqun , Huang Yunjian , Xie Xianhe 
Published: October 2023

Abstract
Background
The association between lipid metabolism disorder and carcinogenesis is well-established, but there is limited research on the connection between lipid metabolism-related genes (LRGs) and lung adenocarcinoma (LUAD). The objective of our research was to identify LRGs as the potential biomarkers for prognosis and assess their impact on immune cell infiltration in LUAD.

Methods
We identified novel prognostic LRGs for LUAD patients via the bioinformatics analysis. CYP27A1 expression level was systematically evaluated via various databases, such as TCGA, UALCAN, and TIMER. Subsequently, LinkedOmics was utilized to perform the CYP27A1 co-expression network and GSEA. ssGSEA was conducted to assess the association between infiltration of immune cells and CYP27A1 expression. CYP27A1’s expression level was validated by qRT-PCR analysis.

Results
CYP27A1 expression was decreased in LUAD. Reduced CYP27A1 expression was linked to unfavorable prognosis in LUAD. Univariate and multivariate analyses indicated that CYP27A1 was an independent prognostic biomarker for LUAD patients. GSEA results revealed a positive correlation between CYP27A1 expression and immune-related pathways. Furthermore, CYP27A1 expression was positively correlated with the infiltration levels of most immune cells.

Conclusion
CYP27A1 is a potential biomarker for LUAD patients, and our findings provided a novel perspective to develop the prognostic marker for LUAD patients.
The preconception health status of nongravid women aged 18 to 45 years in Arima, Trinidad: a cross-sectional study
Authors: Bala Ria , Maharaj Rohan G , Boppana Leela Krishna Teja , Teelucksingh Surujpal 
Published: October 2023

Abstract
Background
Preconception care (PCC) is the term used for activities and interventions designed to address and prevent problems related to pregnancy, the neonatal period and childhood. This study assessed maternal health status prior to conception in Trinidad by means of a screening tool, physical measurements, and laboratory samples.

Methods
A cross-sectional study was conducted among women aged 18–45 years at a primary care centre in Arima, Trinidad. A de novo PCC screening tool was used to assess 13 domains of high-risk pregnancy in participants. These domains included dietary details, gynaecological and obstetric histories, and genetic and vaccination histories, among others. Blood pressure, weight, height, and waist circumference were recorded, and a capillary blood sample was used to determine random blood glucose and HbA1c levels. All data were coded and entered into SPSS ver. 21.

Results
A total of 400 nongravid participants were recruited, of whom 366 were included in the final analysis. Most (96.7%) had one or more risk factors for adverse pregnancy outcomes. These included overweight (27%), obesity (35%), central obesity (69.4%), and impaired glucose tolerance/diabetes mellitus (IGT/DM) (26.2%). Additionally, a sedentary lifestyle and diet high in processed food/fats were self-reported by 74.9% and 88.8% of participants, respectively. Only 13.1% had planned to conceive, and of those who had no immediate plans to conceive, 76.4% were currently sexually active, and many (60.7%) did not use birth control techniques. More than half (57.1%) had never had a pap smear. On the other hand, 86.3% knew their HIV status. Self-reported percentages for vaccination were as follows: MMR (100%), tetanus (17.5%), hepatitis B (11.5%) and influenza (2.7%). The majority (82.8%) of participants had not visited the dentist in the past year, with 35.9% of these individuals reporting symptoms of periodontitis. Segments of the population had multiple risk factors; for example, 23.7% of participants were overweight or obese and had an elevated HbA1c level.

Conclusions
Unexpectedly, most participants had a risk factor for an adverse pregnancy outcome, and many had multiple risk factors. There is a strong case for enhanced preconception care for women in Trinidad.
A systematic review of school-based student peer-led oral health interventions to promote the oral health of school children
Authors: Elsadek Yasmen E. , Edwebi Sakina , Turner Abigail , Vinall-Collier Karen , Csikar Julia , Pavitt Sue 
Published: October 2023

Abstract
Background
Poor oral health in children highlights the need for prevention and effective interventions. During late childhood and adolescence, peer relationships can play a vital role in adopting and maintaining positive health behaviours.

Aim
To identify the oral health outcomes of school-based student peer-led delivery of oral health interventions.

Methods
A search strategy was developed, piloted, and run in four electronic databases: Medline via Ovid, Web of Science, CINAHL via EBSCO, and CENTRAL (Cochrane Central Register of Controlled Trials) using key concepts of peer, oral health and adolescent in the school context. Methodological quality was assessed using QuaDs quality assessment tool. All articles were independently screened by two researchers and data was analysed using narrative data synthesis. The PRISMA checklist complemented by aspects of the Synthesis Without Meta-analysis (SWiM) was used to report this systematic review.

Results
There were 7572 identified, 24 studies progressed to full-text review, ten studies met the eligibility criteria and were included in the review. Only six studies based their interventions on psychological & behavioural theory. Intervention delivered by peers showed improvements in both clinical and self-reported outcomes when compared to other delivery methods (e.g., professionals). Quality of included studies was reported according to QuaDs guidance.

Conclusion
Peer-led interventions were more effective in improving oral health status and behaviours when compared to other modes of delivery. Future research should assess if a bi-directional impact of peer-led interventions can be seen. Specifically, if there is added value for school-based student peer-leader's including their own oral health knowledge, skills, attitude and preventative behaviours.
Predicting perineal trauma during childbirth using data from a general obstetric population
Authors: Maher Gillian M. , O'Byrne Laura J. , McKernan Joye , Corcoran Paul , Greene Richard A. , Khashan Ali S. , McCarthy Fergus P. 
Published: October 2023

Background: Perineal trauma is a common complication of childbirth and can have serious impacts on long-term health. Few studies have examined the combined effect of multiple risk factors. We developed and internally validated a risk prediction model to predict third and fourth degree perineal tears using data from a general obstetric population. Methods: Risk prediction model using data from all singleton vaginal deliveries at Cork University Maternity Hospital (CUMH), Ireland during 2019 and 2020. Third/fourth degree tears were diagnosed by an obstetrician or midwife at time of birth and defined as tears that extended into the anal sphincter complex or involved both the anal sphincter complex and anorectal mucosa. We used univariable and multivariable logistic regression with backward stepwise selection to develop the models. Candidate predictors included infant sex, maternal age, maternal body mass index, parity, mode of delivery, birthweight, post-term delivery, induction of labour and public/private antenatal care. We used the receiver operating characteristic (ROC) curve C-statistic to assess discrimination, and bootstrapping techniques were used to assess internal validation. Results: Of 8,403 singleton vaginal deliveries, 8,367 (99.54%) had complete data on predictors for model development. A total of 128 women (1.53%) had a third/fourth degree tear. Three variables remained in the final model: nulliparity, mode of delivery (specifically forceps delivery or ventouse delivery) and increasing birthweight (per 100 gram increase) (C-statistic: 0.75, 95% CI: 0.71, 0.79). We developed a nomogram to calculate individualised risk of third/fourth degree tears using these predictors. Bootstrapping indicated good internal performance. Conclusions: Use of our nomogram can provide an individualised risk assessment of third/fourth degree tears and potentially aid counselling of women on their potential risk.
Implementing 3rd-year learning disability student nurse-led clinics and evaluating self-efficacy and confidence
Authors: Hodge Gemma , Cross Debbie , Barwick Claire 
Published: October 2023

Background Workplace self-confidence and self-efficacy has been strongly associated with increased coping strategies, job satisfaction and resilience to workplace adversity ( Bandura, 1982 ) which results in higher quality care delivery. The main aim was to evaluate the growth of confidence and autonomy in developing a Learning Disability professional identity within primary care settings, through student led clinics, utilising a long arm supervision approach. Methodology/Methods An explorative hermeneutic phenomenology approach, using semi structured interviews was utilised to explore themes and experiences around self-efficacy and confidence in students, practice assessors and supervisors. Findings Long arm approach is an effective learning approach for students to develop their own autonomy in primary care clinics. However, placement planning and a supportive environment are vital for its success and for the student to build self-efficacy and autonomy in a primary care setting. Conclusion and Implications Increased confidence is viewed as a crucial factor in evolving role identity and managing change which are major factors in the preparation for registration and joining the healthcare workforce. The 3rd year Learning Disability nurse led clinics did have impact on workforce well-being by alleviating workload on quality outcomes and frameworks (QOF); prepared students for autonomous working; and increase practitioners’ confidence in using long arm supervision.