bluuebunny/crossref_metadata_2025_split huggingface dataset:
Topological regulation of the estrogen transcriptional response by ZATT-mediated inhibition of TOP2B activity
Authors: Terrón-Bautista José , Martínez-Sánchez María del Mar , López-Hernández Laura , Vadusevan Ananda Ayyappan , García-Domínguez Mario , Williams R. Scott , Aguilera Andrés , Millán-Zambrano Gonzalo , Cortés-Ledesma Felipe 
Published: January 2024

SUMMARYHuman type-II topoisomerases, TOP2A and TOP2B, remove transcription associated DNA supercoiling, thereby affecting gene-expression programs, and have recently been associated with 3D genome architecture. Here, we study the regulatory roles of TOP2 paralogs in response to estrogen, which triggers an acute transcriptional induction that involves rewiring of genome organization. We find that, whereas TOP2A facilitates transcription, as expected for a topoisomerase, TOP2B limits the estrogen response. Consistent with this, TOP2B activity is locally downregulated upon estrogen treatment to favor the establishment and stabilization of regulatory chromatin contacts, likely through an accumulation of DNA supercoiling. We show that estrogen-mediated inhibition of TOP2B requires estrogen receptor α (ERα), a non-catalytic function of TOP2A, and the action of the atypical SUMO-ligase ZATT. This mechanism of topological transcriptional-control, which may be shared by additional gene-expression circuits, highlights the relevance of DNA topoisomerases as central actors of genome dynamics.
Evaluating the Effects of Environmental Disturbances and Pesticide Mixtures on N-cycle related Soil Microbial Endpoints
Authors: Drocco Camilla , Coors Anja , Devers-Lamrani Marion , Martin-Laurent Fabrice , Rouard Nadine , Spor Aymé 
Published: January 2024

ABSTRACTPesticides are widely used in conventional agriculture, either applied separately or in combination during the culture cycle. Due to their occurrence and persistence in soils, pesticide residues may have an impact on soil microbial communities and on supported ecosystem services. In this regard, the EFSA (European Food Safety Authority) recently published a scientific opinion inciting to change pesticide risk assessment to better protect soil microbe-mediated processes. Climate change is another major concern for all living organisms including soil microbial community stability. Extreme climatic events, such as heat waves or heavy rainfalls, are becoming more and more frequent and their impact on soil microbial diversity and functions have already been demonstrated.The objectives of this study were to evaluate the effects of temperature and humidity disturbances and pesticide active ingredients exposure on soil microbial community structure and functions. To this end, 250 soil microcosms were exposed to either a heat disturbance, a high humidity to mimic heavy rain, or no environmental disturbance. After three days of recovery, soil microcosms were treated with different active ingredients: clopyralid (herbicide), cypermethrin (insecticide) and pyraclostrobin (fungicide). The treatments were applied alone or in combination at 1x or 10x of the agronomical dose. We then evaluated the effects of the disturbances and the active ingredients on various microbial endpoints related to the diversity and the structure of soil microbial communities, and with a specific focus on microbial guilds involved in nitrification.Overall, we demonstrated that the impact of environmental disturbances applied to soil microcosms, especially heat, on microbial endpoints was stronger than that of the active ingredients applied alone or in combinations. Compounded effects of environmental disturbances and active ingredients were detected, but sparsely and were of small scale for the chosen pesticides and applied doses.
3D imaging with superior resolution using Atacama Clear
Authors: Lacko Lauretta A. , Choi Tansol , de Silva Neranjan , Liu Ying , Jamies Edgar A. , Evans Todd , Hurtado Romulo 
Published: January 2024

Abstract3-dimensional (3D) imaging is a powerful tool for interrogation of intact tissues, but can suffer from poor resolution due to impediments such as high tissue autofluorescence that remains a significant challenge in imaging cleared samples, including human clinical specimens. We developed Atacama Clear (ATC), a 3D imaging technology that increases signal-to-noise ratios (SNRs) while simultaneously augmenting the capacity of tissue to be cleared. ATC exhibited SNRs that are up to 200% of widely used 3D imaging methods, potentiated all tested optical clearing solutions by up to 600%, decreased the time of optical clearing by up to a factor of 8, and enabled detection of poorly recognized antigens with a remarkable 4-fold increase in signal detection while using up to 10-fold lower antibody concentrations. Strikingly, ATC produced up to a 5x increase in transgenic fluorescent reporter protein signal detection, which is instead often diminished with currently used 3D imaging methods. This increased imaging efficacy enabled multiplex interrogation of tough fibrous tissue and specimens that naturally exhibit high levels of background noise, including the heart, kidney, and human biopsies. Indeed, ATC facilitated the use of AI based auto-segmentation with simple low tech stereo fluorescence microscopy, visualization of previously undocumented adjacent nephron segments that exhibit notoriously high autofluorescence, elements of the cardiac conduction system, and distinct human glomerular tissue layers, with cellular resolution. Taken together, these studies establish ATC as a platform for complex 3D imaging studies of basic and clinical specimens with superior resolution.
A new insight into the role of CART peptide in serotonergic function and anxiety
Authors: Balasubramanian Nagalakshmi , Wang Ruixiang , Ismail Shafa , Hartman Benjamin , Aboushaar Zeid , Marcinkiewcz Catherine A. 
Published: January 2024

AbstractCocaine and amphetamine-regulated transcript (CART) peptide has been established as a contributor to anxiogenic behavior. Genetic mutations in the CART gene are associated with anxiety and depression, and increased CART expression has been reported in suicide victims. Extensive research has focused on the role of CART peptide in mesolimbic neurocircuitry, but its involvement in the dorsal raphe nucleus (DRN) and serotonin (5HT) system remains unexplored. Here we demonstrate that CART processes are proximal to 5HTDRNneurons and that microinjection of CART(55-102)peptide into the DRN has an anxiogenic effect in mice. Furthermore, central CART administration reduced cfos activation in 5HT neurons of the ventral DRN, which is a putative reward/anti-stress circuit. The inhibitory effect of CART on 5HTDRNneuronal function and local 5HT release is further demonstrated within vivofiber photometry coupled with calcium and 5HT biosensors and by mass spectrometry. Moreover, using Cre-dependent retrograde tracing, we observed DRN-projecting CART neurons in the Edinger Westphal nucleus (EW), nucleus accumbens (NAc), and various hypothalamic nuclei including the ventromedial hypothalamus (VMH). Interestingly, based onex vivoelectrophysiological recordings, acute stress increased excitability in DRN-projecting CART neurons located in the EW, but not in the VMH or NAc. This suggests that the stress may promote anxiety-like behavior by activating the EWCART→5HTDRNcircuit that ultimately inhibits 5HT transmission. In sum, understanding the intricate dynamics of the CARTergic and 5HTergic systems proves crucial in addressing 5HT-related dysfunctions, providing invaluable insights into both health and disease.
Development of differential sublaminar feedforward inhibitory circuits in CA1 hippocampus requiresSatb2
Authors: Hanson Meretta A. , Bibi Noor , Safa Alireza , Nagarajan Devipriyanka , Marshall Alec H. , Johantges Aidan C. , Wester Jason C. 
Published: January 2024

SUMMARYPyramidal cells (PCs) in CA1 hippocampus can be classified by their radial position as deep or superficial and organize into subtype-specific circuits necessary for differential information processing. Specifically, superficial PCs receive fewer inhibitory synapses from parvalbumin (PV)-expressing interneurons than deep PCs, resulting in weaker feedforward inhibition of input from CA3 Schaffer collaterals. Using mice, we investigated mechanisms underlying PC differentiation and the development of this inhibitory circuit motif. We found that expression of the transcriptional regulator SATB2 is biased towards superficial PCs during early postnatal development and necessary to suppress PV+ interneuron synapse formation. In the absence of SATB2, the number of PV+ interneuron synaptic puncta surrounding superficial PCs increases during development to match deep PCs. This results in equivalent inhibitory current strength observed in paired whole-cell recordings, and equivalent feedforward inhibition of Schaffer collateral input. Thus, SATB2 is necessary for superficial PC differentiation and biased feedforward inhibition in CA1.
Reconciling experimental and lattice data of
Zc(3900)
in a
J/ψπ−DD¯*
coupled-channel analysis
Authors: Yan Lin-Wan , Guo Zhi-Hui , Guo Feng-Kun , Yao De-Liang , Zhou Zhi-Yong 
Published: January 2024

We study the J/ψπ and DD¯* coupled-channel system within a covariant framework. The J/ψπ and DD¯* invariant-mass distributions measured at 4.23 and 4.26 GeV by BESIII and the finite-volume energy levels from recent lattice QCD simulations are simultaneously fitted. Phase shifts and inelasticities of the J/ψπ and DD¯* scattering are predicted using the resulting amplitudes. Poles corresponding to the Zc(3900) state are found in the complex energy plane and their couplings with J/ψπ and DD¯* are determined. Our results indicate that the current lattice data do not preclude the existence of the Zc(3900) state.

Published by the American Physical Society
2024
Does expanded disclosure in the audit report involve unintended consequences? Evidence from tax avoidance
Authors: Baatwah Saeed Rabea , Hussainey Khaled 
Published: January 2024

Purpose
This study aims to examine how new regulation changes for the auditor’s report, so-called key audit matters (KAMs), influence tax avoidance.

Design/methodology/approach
This study uses data from firms listed on the Omani capital market over the period 2012–2019 and analyzes these data using pooled panel data regression with a robust standard error. It uses two common proxies for tax avoidance and two measures for the KAMs disclosure requirement.

Findings
This study finds a sharp decrease in the effective tax rate following the introduction of KAMs disclosure and the issuance of more KAMs in audit reports. This result is supported by several robustness checks. In an additional analysis, the authors observe interesting results, indicating that real earnings management mediates this association, while the audit committee plays a moderating role. The authors do not find a moderating effect of Big4 on this association, but find discrepancies within the Big4 firms in relation to this moderating effect.

Originality/value
The results of this study indicate that although the introduction of the KAMs disclosure requirement may have positive consequences, it may also lead to unintended negative consequences. This conclusion has not been comprehensively reported in literature.
How does abusive supervision differentiation affect employee work-family conflict? A moderated chain mediation model
Authors: Zhang Lanxia , Li Jia-Min , Mao Mengyu , Na Lijie 
Published: January 2024

Purpose
This study aims to explore the mechanism of abusive supervision differentiation on employee work-family conflict, and examine the chain mediating role of work-related rumination and organizational citizenship behavior/deviant workplace behavior, as well as the moderating role of work-family boundary segmentation preference.

Design/methodology/approach
The authors designed two studies: Study 1 was a scenario experiment with 120 Master of Business Administration students. To further explore this finding, the authors conducted a multiwave survey in Study 2 with 345 employees from various organizations.

Findings
The results of Study 1 showed that abusive supervision differentiation had a positive effect on work-related rumination, and work-related rumination mediated the relationship between differentiated abusive supervision and organizational citizenship behavior/deviant workplace behavior. The results of Study 2 not only confirmed the conclusions of Study 1 but also revealed that organizational citizenship behavior/deviant workplace behavior significantly affected work-family conflict. Abusive supervision differentiation had a positive effect on work-family conflict through work-related rumination and organizational citizenship behavior/deviant workplace behavior. In addition, work-family boundary segmentation preference negatively moderated the relationship between organizational citizenship behavior and work-family conflict.

Originality/value
First, to the best of the authors’ knowledge, this study is the first paper to test the spillover effect of abusive supervision differentiation on the family domain through a chain mediation model. It extends the research on abusive supervision differentiation from the work domain to the family domain. Second, previous research has highlighted role conflict or role insufficiency as significant factors contributing to work-family conflict. However, this study suggests that abusive supervision differentiation from workplace managers can also trigger work-family conflict, providing a new perspective in the study of precursors to work-family conflict.
Exploring factors affecting communication in three-dimensional virtual worlds for second language learning: development and validation of a scale
Authors: Wiboolyasarin Kanokpan , Wiboolyasarin Watcharapol , Kamonsawad Ruedee , Tiranant Phornrat , Boonyakitanont Poomipat , Jinowat Nattawut 
Published: January 2024

PurposeThe use of three-dimensional virtual worlds (3DVWs) is increasingly becoming a common practice in language education to provide digital learning environments for second-language (L2) communicative classes. This study aimed to identify the key factors underlying communication in 3DVWs that can improve the communication skills of L2 learners.Design/methodology/approachTo achieve this, exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were conducted to validate the identified factors affecting communication in 3DVWs. A self-reported questionnaire with 47 items on a five-point Likert scale was administered to 513 pre-service teachers, teachers and lecturers in the field of language education.FindingsThe results of the EFA revealed four factors that contribute to communication in 3DVWs, namely learner motivation, interaction pattern, language development and learner autonomy. CFA results provided support for the updated model, with statistically significant Chi-square results (χ² (df = 83) = 181.049, p < 0.001) indicating a good fit between the model and the data.Originality/valueThe findings suggest that the four EFA-derived parameters are valid and can assist instructional designers and L2 instructors in creating 3DVWs that enhance L2 learners' communication abilities. This study provides valuable insights for educators, instructional designers and researchers in the field of language education and technology-enhanced learning.
Study on structure optimization and performance improvement of Y-shaped water seal of rock drill
Authors: Jia Chun Qiang , Wang Aofei , Yu Ling , Zong Li 
Published: January 2024

Purpose
The rock drill’s drill tail experiences high-frequency fretting simultaneously in the rotational and axial directions. Due to the complex working characteristics and the low viscosity of the water medium, the pure water seal is susceptible to damage and failure. The purpose of this paper is to enhance the water seal’s performance.

Design/methodology/approach
The Y-shaped seal ring is modeled and simulated using orthogonal testing. Through analysis of the impact of various seal section parameters on sealing performance, the maximum contact stress and maximum Von Mises stress are selected as indicators of sealing effectiveness.

Findings
The maximum contact stress is proportional to lip thickness and chamfer length but inversely proportional to lip length. Meanwhile, the maximum Von Mises stress is directly influenced by lip depth and the included angle of the lip and drill tail but is inversely proportional to the lip thickness. The enhanced Y-shaped water seal sees reductions of 15% and 45% in maximum contact stress and maximum Von Mises stress, respectively.

Originality/value
This paper used analytical method and model that is helpful for design of the water seal’s structure in complex working characteristics and the low viscosity of the water medium.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-11-2023-0366/
Islamic finance as a driver for enhancing economic sustainability and innovation in the GCC
Authors: Alhammadi Salah 
Published: January 2024

Purpose
This study aims to investigate the role of Islamic finance in supporting sustainable economic growth, innovation and digital transformation in the Gulf Cooperation Council (GCC) region. Amid global challenges like the Russia–Ukraine conflict and COVID-19, the focus extends beyond the GCC’s oil dependency to explore how Islamic finance can enable technological advancements and foster a digitally innovative economy. The research aims to reveal the potential of Islamic finance in driving economic diversification, technological progress and sustainable development in the GCC.

Design/methodology/approach
Using a content analysis approach, this study critically examines the economic repercussions of recent global crises, shedding light on how Islamic finance contributes to socio-economic justice and the provision of social goods in the GCC. The research synthesises findings from various secondary sources, including academic literature, reports and industry standards, to analyse Islamic finance’s role from an ethical and strategic perspective within the GCC’s evolving economic landscape.

Findings
The findings reveal Islamic finance’s potential to significantly contribute to the GCC’s economic diversification and resilience against global economic downturns. The study highlights how Islamic finance aligns with the sustainable development goals and its effectiveness in promoting ethical financial practices and socio-economic justice.

Research limitations/implications
Future research should focus on global comparative studies to understand Islamic finance’s impact on sustainable development beyond the GCC. Longitudinal studies are also essential to assess the long-term effects of Islamic financial instruments on economic stability.

Practical implications
The research advocates for incorporating Islamic finance principles into the GCC’s economic strategies, emphasising its role in providing resilient and ethical financial alternatives conducive to sustainable development. It underscores the need for policy initiatives integrating Islamic finance to bolster socio-economic welfare and environmental sustainability.

Originality/value
Offering a novel perspective, this paper enriches the discourse on the contribution of Islamic finance to sustainable economic development. It presents critical insights into how Islamic finance can underpin long-term economic resilience and growth in the GCC. It provides valuable implications for academia and policymaking, particularly in emerging economies’ science and technology policy management.
Acceptability of home-based transcranial direct current stimulation (tDCS) in major depression: a qualitative analysis of individual experiences
Authors: Rimmer Rachael M. , Woodham Rachel D. , Cahill Sharon , Fu Cynthia H.Y. 
Published: January 2024

Purpose
The purpose of this paper was to gain a qualitative view of the participant experience of using home-based transcranial direct current stimulation (tDCS). Acceptability impacts patient preference, treatment adherence and outcomes. However, acceptability is usually assessed by rates of attrition, while multifaceted constructs are not reflected or given meaningful interpretations. tDCS is a novel non-invasive brain stimulation that is a potential treatment for major depressive disorder (MDD). Most studies have provided tDCS in a research centre. As tDCS is portable, the authors developed a home-based treatment protocol that was associated with clinical improvements that were maintained in the long term.

Design/methodology/approach
The authors examined the acceptability of home-based tDCS treatment in MDD through questionnaires and individual interviews at three timepoints: baseline, at a six-week course of treatment, and at six-month follow-up. Twenty-six participants (19 women) with MDD in a current depressive episode of at least moderate severity were enrolled. tDCS was provided in a bifrontal montage with real-time remote supervision by video conference at each session. A thematic analysis was conducted of the individual interviews.

Findings
Thematic analysis revealed four main themes: effectiveness, side effects, time commitment and support, feeling held and contained. The themes reflected the high acceptability of tDCS treatment, whereas the theme of feeling contained might be specific to this protocol.

Originality/value
Qualitative analysis methods and individual interviews generated novel insights into the acceptability of tDCS as a potential treatment for MDD. Feelings of containment might be specific to the present protocol, which consisted of real-time supervision at each session. Meaningful interpretation can provide context to a complex construct, which will aid in understanding and clinical applications.
The relationship between weight status and food environments, peer influence and dietary intake among high-school students: a case-control study
Authors: Mohabati Shabnam , Mirahmadizadeh Alireza , Hassanzadeh-Rostami Zahra , Bellissimo Nick , Faghih S. 
Published: January 2024

Purpose
The purpose of this study is to evaluate the relationship between weight status and food environments, peer influence and dietary intake among high-school students in Shiraz, Iran.

Design/methodology/approach
Applying a case-control design, 406 adolescents (n = 203 overweight or obese and n = 203 normal weight) aged 14–18 years were selected using a multistage cluster random sampling method. Demographic information, physical activity level and anthropometric indices were collected. Dietary intake was determined using a 147-item food frequency questionnaire. Food environment (home and out of home) and peer influence were determined by a validated questionnaire.

Findings
The type of food purchased using pocket money was different between adolescents with overweight or obesity and normal weight adolescents (p < 0.001). The out-of-home food environment score was not different between groups, but the home food environment score (p = 0.004) and the peer influence score (p = 0.001) were higher in normal weight adolescents. Adolescents with overweight or obesity consumed higher amounts of carbohydrate (p = 0.006) and lower amounts of protein (p = 0.01) and more sweet junk foods (p = 0.01), nonstarchy vegetables (p = 0.03) and fruits (p = 0.01) compared to the normal weight group.

Originality/value
Home food environment, peer influence, differences in macronutrient intake and dietary patterns may be contributing factors to adolescent weight status.