bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities
Authors: ROBERTS Scott J. , STEWART Alan J. , SADLER Peter J. , FARQUHARSON Colin 
Published: August 2004

Human PHOSPHO1 is a phosphatase enzyme for which expression is upregulated in mineralizing cells. This enzyme has been implicated in the generation of Pi for matrix mineralization, a process central to skeletal development. PHOSPHO1 is a member of the haloacid dehalogenase (HAD) superfamily of Mg2+-dependent hydrolases. However, substrates for PHOSPHO1 are, as yet, unidentified and little is known about its activity. We show here that PHOSPHO1 exhibits high specific activities toward phosphoethanolamine (PEA) and phosphocholine (PCho). Optimal enzymic activity was observed at approx. pH 6.7. The enzyme shows a high specific Mg2+-dependence, with apparent Km values of 3.0 μM for PEA and 11.4 μM for PCho. These results provide a novel mechanism for the generation of Pi in mineralizing cells from PEA and PCho.
Characterization of a sterol carrier protein 2/3-oxoacyl-CoA thiolase from the cotton leafworm (Spodoptera littoralis): a lepidopteran mechanism closer to that in mammals than that in dipterans
Authors: TAKEUCHI Hajime , CHEN Jian-Hua , JENKINS John R. , BUN-YA Masanori , TURNER Philip C. , REES Huw H. 
Published: August 2004

Numerous invertebrate species belonging to several phyla cannot synthesize sterols de novo and rely on a dietary source of the compound. SCPx (sterol carrier protein 2/3-oxoacyl-CoA thiolase) is a protein involved in the trafficking of sterols and oxidation of branched-chain fatty acids. We have isolated SCPx protein from Spodoptera littoralis (cotton leafworm) and have subjected it to limited amino acid sequencing. A reverse-transcriptase PCR-based approach has been used to clone the cDNA (1.9 kb), which encodes a 57 kDa protein. Northern blotting detected two mRNA transcripts, one of 1.9 kb, encoding SCPx, and one of 0.95 kb, presumably encoding SCP2 (sterol carrier protein 2). The former mRNA was highly expressed in midgut and Malpighian tubules during the last larval instar. Furthermore, constitutive expression of the gene was detected in the prothoracic glands, which are the main tissue producing the insect moulting hormone. There was no significant change in the 1.9 kb mRNA in midgut throughout development, but slightly higher expression in the early stages. Conceptual translation of the cDNA and a database search revealed that the gene includes the SCP2 sequence and a putative peroxisomal targeting signal in the C-terminal region. Also a cysteine residue at the putative active site for the 3-oxoacyl-CoA thiolase is conserved. Southern blotting showed that SCPx is likely to be encoded by a single-copy gene. The mRNA expression pattern and the gene structure suggest that SCPx from S. littoralis (a lepidopteran) is evolutionarily closer to that of mammals than to that of dipterans.
Thymic T Cell Development and Progenitor Localization Depend on CCR7
Authors: Misslitz Ana , Pabst Oliver , Hintzen Gabriele , Ohl Lars , Kremmer Elisabeth , Petrie Howard T. , Förster Reinhold 
Published: August 2004

T cell differentiation in the adult thymus depends on sequential interactions between lymphoid progenitors and stromal cells found in distinct regions of the cortex and medulla. Therefore, migration of T cell progenitors through distinct stromal environments seems to be a crucial process regulating differentiation and homeostasis inside the thymus.
Here we show that CCR7-deficient mice are distinguished by a disturbed thymic architecture, impaired T cell development, and decreased numbers of the thymocytes. Analysis of developing double negative (CD4−CD8−) pool of wild-type thymus reveals that CCR7 expression is restricted to a CD25intCD44+ subpopulation. Correspondingly, CCR7 deficiency results in an accumulation of this population in mutant thymus. Furthermore, immunohistology shows that in CCR7-deficient mice CD25+CD44+ cells accumulate at the cortico-medullary junction, suggesting that CCR7 signaling regulates the migration of early progenitors toward the outer thymic cortex, thereby continuing differentiation. Results obtained from mixed bone marrow chimeras support this view, since the development of CCR7-deficient thymocytes is also disturbed in a morphologically intact thymus. Thus, our findings establish an essential role for CCR7 in intrathymic migration and proper T cell development.
Photogrammetric Reconstruction of the Great Buddha of Bamiyan, Afghanistan
Authors: Grün Armin , Remondino Fabio , Zhang Li 
Published: August 2004

AbstractIn the valley of Bamiyan, Afghanistan, approximately 1700 years ago, two large standing Buddha statues were carved out of the sedimentary rock of the region. They were 53 and 38 m high and the larger one figured as the tallest representation of a standing Buddha in the world. In March 2001 the Taleban government militia demolished the colossal statues. After the destruction a group from ETH Zürich completed the computer reconstruction of the Great Buddha, which can serve as the basis for a physical reconstruction. This paper reports the results of the image‐based 3D reconstruction of the statue, performed on three different data‐sets in parallel and using different photogrammetric techniques and algorithms.
CREDIBILITY, MACROECONOMIC FUNDAMENTALS AND MARKOV REGIME SWITCHES IN THE EMS
Authors: Sarantis Nicholas , Piard Sylviane 
Published: August 2004

AbstractThe main objective of this paper is to use the Markov regime‐switching modelling framework to describe and analyse the credibility of a number of countries participating in the European Monetary System during 1980–1998. Our credibility indicator, based on Hughes Hallet et al.'s (1997) methodology, is subject to discrete regime shifts and is made dependent on macroeconomic fundamentals. We carry out extensive testing to assess the specification of the Markov regime‐switching model and the potential existence of permanent breaks. A contribution of our paper is the specification of a multivariate Markov switching model that allows us to examine whether macroeconomic variables have asymmetric effects on credibility. Another contribution is the specification of a regime‐switching model with time‐varying transition probabilities, which enables us to determine whether changes in macroeconomic variables can trigger switches between the low and high credibility regimes. We find strong evidence of regime switching behaviour in all countries. Both the level of credibility and the transition probabilities display an asymmetric response to changes in macroeconomic variables, with the stance of fiscal policy exerting the most systematic influence in all countries.
IS THE VIEW FROM THE EUROTOWER PURELY EUROPEAN? – NATIONAL DIVERGENCE AND ECB INTEREST RATE POLICY
Authors: Heinemann Friedrich , Huefner Felix P. 
Published: August 2004

AbstractThe official view on ECB monetary policy claims that decisions are based on euro zone data and that diverging regional developments are disregarded. To test empirically whether regional developments have an impact on ECB decisions we develop a generalised monetary policy reaction function which allows for an influence of regional divergence. Reaction function estimations and a probit model of interest rate decisions for the first years of the euro area offer some first support for an impact of regional divergence. The results clarify that ignoring a potential national perspective may lead to biased estimates for the ECB reaction function.
Cation binding by acid‐washed peat, interpreted with Humic Ion‐Binding Model VI‐FD
Authors: Smith E. J. , Rey‐Castro C. , Longworth H. , Lofts S. , Lawlor A. J. , Tipping E. 
Published: August 2004

SummaryA sample of ombrotrophic peat from Moor House in northern England was extensively extracted with dilute nitric acid (pH 1) to free it of bound cations. Suspensions of the acid‐washed peat (5–30 g l−1), prepared with different concentrations of background electrolyte (NaCl and KCl), were used to conduct batch acid–base titrations. A strong dependence of proton release on ionic strength (I) was observed, the apparent acid dissociation constant (pKapp) being found to decrease by approximately 1.0 for each tenfold increase in I. This behaviour could not be explained satisfactorily with Humic Ion‐Binding Model VI, a discrete‐site/electrostatic model of cation binding by humic substances, parameterized with data from laboratory studies on isolated samples. More success was obtained by abandoning the impermeable‐sphere electrostatic submodel used in Model VI, and instead assuming the peat to consist of aggregates with fixed internal volume, and with counterion accumulation described by the Donnan model, as proposed by Marinsky and colleagues. The fixed‐volume Donnan model (Model VI‐FD) could also approximately explain other reported results from acid–base titrations of peat, including the effects on the titrations of complexing cations (Al, Ca, Cu). Copper titrations of the Moor House sample were performed using an ion‐selective electrode, with peat suspensions in the acid pH range, at two ionic strengths, and in the presence of Al and Ca. The measured concentrations of Cu2+ were in the range 10−13−10−5 m. Model VI‐FD provided reasonable fits of the experimental data, after optimization of the intrinsic binding constant for Cu, the optimized value being close to the default value derived previously from data referring to isolated humic substances. The optimized constants for Al and Ca, derived from their competition effects, were also close to their default values. Additional experiments were performed in which the centrifugation‐depletion method was used to measure the binding of a cocktail of metals (Al, Ni, Cu, Zn, Cd, Eu, Pb) at a single pH. The model correctly predicted strong binding of Al, Cu, Eu and Pb, and weaker binding of Ni, Zn and Cd. For the strongly binding metals, the dissolved forms were calculated to be mainly due to complexes with dissolved humic matter, whereas the free ions (Ni2+, Zn2+, Cd2+) dominated for the weakly binding metals. Acid‐washed soil appears to provide a valuable intermediate between isolated humic substances and untreated soil for the investigation of cation binding by natural organic matter in the natural environment.
Anticlastogenic, antitoxic and sorption effects of humic substances on the mutagen maleic hydrazide tested in leguminous plants
Authors: Ferrara G. , Loffredo E. , Senesi N. 
Published: August 2004

SummaryThe potential anticlastogenic and antitoxic effects of a soil humic acid (HA), a peat HA and a peat fulvic acid (FA) on the mutagen maleic hydrazide (MH) have been investigated in two legume species, Vicia faba and Pisum sativum. Both HAs and FA were tested at two different concentrations, 20 and 200 mg l−1, either alone or after 24‐hour interaction with 10 mg l−1 of MH before addition to the legume seeds. Anticlastogenicity, i.e. an antimutagenic action defined as the capacity for minimizing chromosome breakages, was evaluated by counting both micronuclei (MN) and aberrant anatelophases (AAT) in root‐tip cells. Length and dry weight of the seedling primary root were measured to test the antitoxic activity of HA and FA on MH. The possible occurrence and extent of adsorption or desorption of MH onto or from HA were also investigated. The two species responded differently to the anticlastogenic tests, with V. faba showing a greater number of MN and AAT anomalies than P. sativum. Peat HA and FA exhibited anticlastogenic and antitoxic activities of similar intensity and greater than those of soil HA. The adsorption capacity of both HAs for MH was small, thus suggesting that adsorption is not a major mechanism responsible for the reduction of clastogenicity and antitoxicity of MH by HA.
Soil physical properties affected by long‐term fertilization
Authors: Pernes‐Debuyser A. , Tessier D. 
Published: August 2004

SummaryAccording to the literature, soil physical properties are linked mainly with organic constituents that are often considered as the first indicator of soil fertility. But the use of fertilizers and amendments can change soil properties independently of the organic matter content. In a long‐term experiment at Versailles, fertilizers and amendments have been applied each year to uncultivated plots. After 70 years, the plots had the same low organic matter content except those which were treated with manure. However, the physico‐chemical environment had become strongly differentiated. Physical properties, especially soil water relations, were also greatly affected. The use of ammoniacal fertilizers strongly decreased soil pH and cation exchange capacity (CECsoil). Plots treated in this way were more sensitive to the degradation of their hydraulic properties and became unstable in spite of the preservation of their porosity. Basic amendments (i.e. bases added as CO32–, OH–, O2– or silicate anions) increased soil pH, CECsoil and its saturation by exchangeable calcium. The increase in CECsoil improved soil structural cohesion and water flow properties. After basic treatment, there is greater structural stability, and water moves faster through the soil. In potassic and sodic plots, K+ and Na+ affected water movement and increased the soil's sensitivity to degradation. Manure treatment increased water retention and soil stability. The cation exchange capacity, measured at soil pH, can be used as a good indicator of soil stability, in combination with the organic matter content and the kinds of exchangeable cation (especially K+ and Na+).
Soil invertebrate activity in biological crusts on tropical inselbergs
Authors: VaÇulik A. , Kounda‐Kiki C. , Sarthou C. , Ponge J. F. 
Published: August 2004

SummaryGranite inselbergs protrude from forest and savanna in the tropics. They are exposed to harsh climates (alternation of heavy rain and severe drought) and provide little nutrient for plants. Soil animals and humus components were investigated in cyanobacterial crusts close to patches of epilithic vegetation on the surface of the Nouragues inselberg (French Guiana). Three biological crust samples, corresponding to bromeliacean carpets of increasing size (supposed of increasing age), were sampled for faunal and micromorphological studies. Arthropods (mainly mites and insects) were abundant and highly diversified, the more so after enchytraeid worms ate and transformed the cyanobacterial mass. Below the superficial cyanobacterial crust, humus was made of a loose assemblage of enchytraeid faeces where these animals were present, or of a compact assemblage of cyanobacteria and amorphous organic matter where mites were the dominant animal group. Roots abounded in the humified part of the crust. We conclude that soil invertebrates, in particular enchytraeid worms, are important for the accumulation of organic matter on granite outcrops, and so therefore for the encroachment of plant succession.
Podzolization as a deferralitization process: a study of an Acrisol–Podzol sequence derived from Palaeozoic sandstones in the northern upper Amazon Basin
Authors: Do Nascimento N.R. , Bueno G.T. , Fritsch E. , Herbillon A.J. , Allard Th. , Melfi A.J. , Astolfo R. , Boucher H. , Li Y. 
Published: August 2004

SummaryMorphological, geochemical and mineralogical studies were carried out in a representative soil catena of the low‐elevation plateaux of the upper Amazon Basin to interpret the steps and mechanisms involved in the podzolization of low‐activity clay soils. The soils are derived from Palaeozoic sandstones. They consist of Hydromorphic Podzols under tree savannah in the depressions of the plateaux and predominantly of Acrisols covered by evergreen forest elsewhere.Incipient podzolization in the uppermost Acrisols is related to the formation of organic‐rich A and Bhs horizons slightly depleted in fine‐size particles by both mechanical particle transfer and weathering. Weathering of secondary minerals by organic acids and formation of organo‐metallic complexes act simultaneously over short distances. Their vertical transfer is limited. Selective dissolution of aluminous goethite, then gibbsite and finally kaolinite favour the preferential cheluviation of first Fe and secondly Al. The relatively small amount of organo‐metallic complexes produced is related to the quartzitic parent materials, and the predominance of Al over Fe in the spodic horizons is due to the importance of gibbsite in these low‐activity clay soils.Morphologically well‐expressed podzols occur in strongly iron‐depleted topsoils of the depression. Mechanical transfer and weathering of gibbsite and kaolinite by organic acids is enhanced and leads to residual accumulation of sands. Organo‐metallic complexes are translocated in strongly permeable sandy horizons and impregnate at depth the macro‐voids of embedded soil and saprolite materials to form the spodic Bs and 2BCs horizons. Mechanical transfer of black particulate organic compounds devoid of metals has occurred later within the sandy horizons of the podzols. Their vertical transfer has formed well‐differentiated A and Bh horizons. Their lateral removal by groundwater favours the development of an albic E horizon. In an open and waterlogged environment, the general trend is therefore towards the removal of all the metals that have initially accumulated as a response to the ferralitization process and have temporarily been sequestrated in organic complexes in previous stages of soil podzolization.
A new model for the soil‐water retention curve that solves the problem of residual water contents
Authors: Groenevelt P. H. , Grant C. D. 
Published: August 2004

SummaryWe present a new model for the soil‐water retention curve, θ(hm), which, in contrast to earlier models, anchors the curve at zero water content and does away with the unspecified residual water content. The proposed equation covers the complete retention curve, with the pressure head, hm, stretching over approximately seven orders of magnitude. We review the concept of pF from its origin in the papers of Schofield and discuss what Schofield meant by the ‘free energy, F ’. We deal with (historical) criticisms regarding the use of the log scale of the pressure head, which, unfortunately, led to the apparent demise of the pF. We espouse the advantages of using the log scale in a model for which the pF is the independent variable, and we present a method to deal with the problem of the saturated water content on the semi‐log graph being located at a pF of minus infinity. Where a smaller range of the water retention is being considered, the model also gives an excellent fit on a linear scale using the pressure head, hm, itself as the independent variable. We applied the model to pF curves found in the literature for a great variety of soil textures ranging from dune‐sand to river‐basin clay. We found the equation for the model to be capable of fitting the pF curves with remarkable success over the complete range from saturation to oven dryness. However, because interest generally lies in the plant‐available water range (i.e. saturation, θs, to wilting point, θwp), the following relation, which can be plotted on a linear scale, is sufficient for most purposes: , where k0, k1 and n are adjustable fitting parameters.
Scale‐ and location‐dependent correlation of nitrous oxide emissions with soil properties: an analysis using wavelets
Authors: Lark R. M. , Milne A. E. , Addiscott T. M. , Goulding K. W. T. , Webster C. P. , O'Flaherty S. 
Published: August 2004

SummaryThis paper shows how the wavelet transform can be used to analyse the complex spatial covariation of the rate of nitrous oxide (N2O) emissions from the soil with soil properties that are expected to control the evolution of N2O. We use data on N2O emission rates from soil cores collected at 4‐m intervals on a 1024‐m transect across arable land at Silsoe in England. Various soil properties, particularly those expected to influence N2O production in the soil, were also determined on these cores.We used the adapted maximal overlap discrete wavelet transform (AMODWT) coefficients for the N2O emissions and soil variables to compute their wavelet covariances and correlations. These showed that, over the transect as a whole, some soil properties were significantly correlated with N2O emissions at fine spatial scales (soil carbon content), others at intermediate scales (soil water content) and others at coarse spatial scales (soil pH). Ammonium did not appear to be correlated with N2O emissions at any scale, suggesting that nitrification was not a significant source of N2O from these soils in the conditions that pertained at sampling.We used a procedure to detect changes in the wavelet correlations at several spatial scales. This showed that certain soil properties were correlated with N2O emissions only under certain conditions of topography or parent material. This is not unexpected given that N2O is generated by biological processes in the soil, so the rate of emission may be subject to one limiting factor in one environment and a different factor elsewhere. Such changes in the relationship between variables from one part of the landscape to another is not consistent with the geostatistical assumption that our data are realizations of coregionalized random variables.
The concentrations of fatty acids in organo‐mineral particle‐size fractions of a Chernozem
Authors: Jandl G. , Leinweber P. , Schulten H.‐R. , Eusterhues K. 
Published: August 2004

SummaryFatty acids, the most abundant class of soil lipids, indicate pedogenetic processes and soil management. However, their quantitative distribution in organo‐mineral particle‐size fractions is unknown. The concentrations of n‐C10:0 to n‐C34:0 fatty acids both in whole soil samples and in the organo‐mineral particle‐size fractions of the Ap horizon of a Chernozem were determined (i) to evaluate the effects of long‐term fertilization and (ii) to investigate their influence on the aggregation of organo‐mineral primary particles. Quantification by gas chromatography/mass spectrometry (GC/MS) showed that long‐term fertilization with nitrogen, phosphorus and potassium (NPK) and farmyard manure (FYM) led to larger concentrations (25.8 µg g−1) of fatty acids than in the unfertilized sample (22.0 µg g−1). For particle‐size fractions of the unfertilized soil, the fatty acid concentrations increased from the coarse silt to the clay fractions (except for fine silt). Fertilization with NPK and FYM resulted in absolute enrichments of n‐C21:0 to n‐C34:0 fatty acids with a maximum at n‐C28:0 in clay (×2.2), medium silt (×2.0), coarse silt (×1.8) and sand (×2.9) compared with the unfertilized treatment (the factors of enrichment are given in parentheses). New evidence for the aggregate stabilizing function of n‐C21:0 to n‐C34:0 fatty acids was shown by the characteristic pattern in size‐fractionated, disaggregated and aggregated samples. Highly significant correlations of fatty acid concentrations with organic C concentrations and specific surface areas are interpreted as indicators of (i) trapping of fatty acids in organic matter macromolecules and (ii) direct bonding to mineral surfaces. This interpretation was supported by the thermal volatilization and determination of fatty acids by pyrolysis‐field ionization mass spectrometry (Py‐FIMS).