bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of suitable reference genes for expression profile analyses of target genes in the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae)
Authors: Zhang Shaohua , Zhu Enhang , Wang Zheng , Zhong Yaofeng , Zha Xuezong , Ji Hengqing , Meng Qianqian 
Published: January 2024

AbstractThe coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a major destructive insect pest of coffee, which impacts the coffee crops negatively. As a draft genome has been completed for this insect, most molecular studies on gene transcriptional levels under different experimental conditions will be conducted using real-time reverse-transcription quantitative polymerase chain reactions (RT-qPCR). However, the lack of suitable internal reference genes will affect the accuracy of RT-qPCR results. In this study, the expression stability of nine candidate reference genes was evaluated under different developmental stages, temperature stress, and Beauveria bassiana infection. Data analyses were completed by four commonly used programs, BestKeeper, NormFinder, geNorm, and RefFinder. The result showed that RPL3 and EF1α combination were recommended as the most stable reference genes for developmental stages. EF1α and RPS3a combination were the top two stable reference genes for B. bassiana infection. RPS3a and RPL3 combination performed as the optimal reference genes both in temperature stress and all samples. Our results should provide a good foundation for the expression profile analyses of target genes in the future, especially for molecular studies on insect genetic development, temperature adaptability, and immune mechanism to entomogenous fungi in H. hampei.
Global Maoism and the Decolonization of China’s History
Authors: Evans James Gethyn 
Published: January 2024

Charu Mazumdar seemed like an unlikely leader for a violent guerilla organization. Born into a family of landlords in India’s West Bengal in 1918, his slender frame gave him the look of someone more used to studying than directing armed insurgency. Yet, Mazumdar justified his violent leadership in West Bengal during the 1960s and 1970s by referencing the writings of Mao Zedong – known collectively as Mao Zedong Thought or Maoism – as inspiration for his revolutionary actions. Mazumdar declared that ‘the foremost duty of [Indian] revolutionaries is to spread and propagate the thought of Chairman Mao’, and that ‘China’s path is our path, China’s chairman is our chairman.’ While Mazumdar had no claim to Chinese ethnic or linguistic belonging, his activities – along with the actions of thousands of others – manifested as a result of the transnational connections and entanglements between Maoism, its translation and propagation by the Chinese Communist Party (CCP), and its reception by revolutionaries across the world.
Characterization of the violence between Türkiye and the PKK
Authors: Kurtuluş Şehmus 
Published: January 2024

AbstractThis article addresses the question of whether the violence between Türkiye and the Kurdistan Worker’s Party (PKK) constitutes an armed conflict within the meaning of international humanitarian law. The article first explains the different non-international armed conflict descriptions provided by (i) the ICTY’s famous Tadić decision, (ii) Additional Protocol II, and (iii) the Rome Statute of the ICC and discusses the different applicability thresholds set by these sources. After noting that the terrorist nature of the acts of violence in a situation will not prevent them from being characterized as an armed conflict and that the application of the international humanitarian law norms would not in fact affect the legal status of the PKK, this article examines whether Türkiye’s struggle against the PKK could be classified as an armed conflict subject to international humanitarian law norms.
Is rape a crime in Japan?
Authors: Johnson David T. 
Published: January 2024

Abstract
Japan is often said to have one of the lowest rape rates in the world, and Japanese police claim to solve 97 percent of rape cases. But in reality, only 5–10 percent of rape victims report it to police, and police record half or less of reported cases while prosecutors charge about one-third of recorded cases. The result of this process of caseload attrition is that for every 1,000 rapes in Japan, only 10–20 result in a criminal conviction – and fewer than half of convicted rapists are incarcerated. Similar patterns characterize Japan's criminal justice response to other sex crimes. This article shows that impunity for sex offenders is extremely common in Japan, and it argues that patriarchal social and legal norms help explain this pattern.
Wideband design and modeling of efficient multi-resonance reflective linear-to-circular polarization converter
Authors: Zaker Reza , Jolani Farid 
Published: January 2024

AbstractA reflective linear-to-circular polarization converter based on dual frequency-selective structures (FSSs) is proposed and modeled to exhibit efficient wideband performance. The design utilizes a diagonal array of two connected circular patches as an effective anisotropy with regular current distribution in several successive resonances, resulting in orthogonal reflections with a 90° phase difference. The relevant upper-part characteristic is improved by using two separate square patches as a high-frequency resonator. This design with distinct key parameters leads to high overlapping and then excellent bandwidth and efficiency over 105% and 96%, respectively, with an axial ratio below 1.7 dB. A sophisticated equivalent circuit-admittance model including effective mutual coupling between two FSSs is extracted, featuring closed-form equations for the physical design. Different dielectric constants are studied on the converter, which offer controllable coverage in the range of 3–24 GHz (S, C, X, Ku, and K bands), variably. For actual validation, a very thin (0.04λ0 at 3.65 GHz) 8 × 8 array prototype was built and measured at different incident angles, showing angular stability up to 45° in 78% (6–14 GHz) bandwidth. This converter has potential applications in communication, spectroscopy, detection, and imaging in micro-, mm-, and THz-wave regions.
A Hybrid Gain Analog Offline EnKF for Paleoclimate Data Assimilation
Authors: Sun Haohao , Lei Lili , Liu Zhengyu , Ning Liang , Tan Zhe‐Min 
Published: January 2024

AbstractFor Paleoclimate data assimilation (PDA), a hybrid gain analog offline ensemble Kalman filter (HGAOEnKF) is proposed. It keeps the benefits of the analog offline ensemble Kalman filter (AOEnKF) that constructs analog ensembles from existing climate simulations with joint information of the proxies. The analog ensembles can provide more accurate prior ensemble mean and “flow‐dependent” error covariances than randomly sampled ensembles. HGAOEnKF further incorporates the benefits of static prior error covariances that better capture large‐scale error correlations and mitigate sampling errors than the sample prior error covariances, through a hybrid gain approach within an ensemble framework. Observing system simulation experiments are conducted for various data assimilation methods, using ensemble simulations from the Community Earth System Model‐Last Millennium Ensemble Project. Results show that using the static prior error covariances estimated from a sufficiently large sample set is beneficial for the traditional offline ensemble Kalman filter (OEnKF) and AOEnKF. HGAOEnKF method is superior to the OEnKF and AOEnKF with and without static prior error covariances, especially for the reconstruction of extreme events. The advantages of HGAOEnKF over OEnKF and AOEnKF with and without static prior error covariances are persistent with varying sample sizes and presence of model errors.
Tradeoffs Between Temporal and Spatial Pattern Calibration and Their Impacts on Robustness and Transferability of Hydrologic Model Parameters to Ungauged Basins
Authors: Demirel M. C. , Koch J. , Rakovec O. , Kumar R. , Mai J. , Müller S. , Thober S. , Samaniego L. , Stisen S. 
Published: January 2024

AbstractOptimization of spatially consistent parameter fields is believed to increase the robustness of parameter estimation and its transferability to ungauged basins. The current paper extends previous multi‐objective and transferability studies by exploring the value of both multi‐basin and spatial pattern calibration of distributed hydrologic models as compared to single‐basin and single‐objective model calibrations, with respect to tradeoffs, performance and transferability. The mesoscale Hydrological Model (mHM) is used across six large central European basins. Model simulations are evaluated against streamflow observations at the basin outlets and remotely sensed evapotranspiration patterns. Several model validation experiments are performed through combinations of single‐ (temporal evaluation through discharge) and multi‐objective (temporal and spatial evaluation through discharge and spatial evapotranspiration patterns) calibrations with holdout experiments saving alternating basins for model evaluation. The study shows that there are very minimal tradeoffs between spatial and temporal performance objectives and that a joint calibration of multiple basins using multiple objective functions provides the most robust estimations of parameter fields that perform better when transferred to ungauged basins. The study indicates that particularly the multi‐basin calibration approach is key for robust parametrizations, and that the addition of an objective function tailored for matching spatial patterns of ET fields alters the spatial parameter fields while significantly improving the spatial pattern performance without any tradeoffs with discharge performance. In light of model equifinality, the minimal tradeoff between spatial and temporal performance shows that adding spatial pattern evaluation to the traditional temporal evaluation of hydrological models can assist in identifying optimal parameter sets.
Downslope Weakening of Soil Revealed by a Rapid Robotic Rheometer
Authors: Ruck John G. , Wilson Cristina G. , Shipley Thomas , Koditschek Daniel , Qian Feifei , Jerolmack Douglas 
Published: January 2024

AbstractMoving down a hillslope from ridge to valley, soil develops and becomes increasingly weathered. Downslope variation in clay content, organic matter, and porosity should produce concomitant changes in soil strength that influence slope stability and erosion. This has yet to be demonstrated, however, because in situ measurements of soil rheology are challenging and rare. Here we employ a robotic leg as a mechanically sensitive and time‐efficient penetrometer to map soil strength along a canonical temperate hillslope profile. We observe a systematic downslope weakening, and increasing heterogeneity, of soil strength associated with a transition from sand‐rich ridge materials to cohesive valley bottom soil aggregates. Weathering‐induced changes in soil composition lead to physically distinct mechanical behaviors in cohesive soils that depart from the behavior observed for sand. We also demonstrate the promise that legged robots may use their limbs to sense and improve mobility in complex environments, with implications for planetary exploration.
Spatial Patterns and Controls on Wind Erosion in the Great Basin
Authors: Treminio Ronald S. , Webb Nicholas P. , Edwards Brandon L. , Faist Akasha , Newingham Beth , Kachergis Emily 
Published: January 2024

AbstractThe Great Basin of the western United States is experiencing dramatic increases in wildfire and Bromus species invasion that potentially accelerate wind erosion and plant community change. We used a wind erosion model parameterized for rangelands and standard ecological monitoring data sets collected at 10,779 locations from 2011 to 2019 to characterize potential wind erosion in the Great Basin, assess relationships between factors affecting wind erosion, and quantify effects of wildfire and invasive Bromus species on aeolian horizontal sediment flux, Q. There were 403 monitoring plots (∼3.7% of plots) with Q > 100 g m−1 d−1. Median values for the highest Q category (>100) ranged from 196.5 to 308.5 g m−1 d−1. Locations with Q > 100 g m−1 d−1 were associated with dry, low elevation areas of the Great Basin with low perennial grass and perennial forb cover, and with large bare gaps between plants. Areas with high perennial grass, perennial forb, and shrub cover had small Q (≤10 g m−1 d−1). Substantial wind erosion was predicted in areas that have experienced wildfires, but areas with multiple wildfires had a lower predicted probability of Q particularly as invasive Bromus species cover increased. Modeled Q was up to two orders of magnitude higher post‐wildfire (median 44.2 g m−1 d−1) than in intact or annual grass‐invaded regions of the Great Basin (median 0.4 g m−1 d−1). Our results reveal the complex interplay among plant community composition, wildfire, and the amount of bare ground controlling wind erosion on Great Basin rangelands.
Harnessing upregulated E-selectin while enhancing SDF-1α sensing redirects infused NK cells to the AML-perturbed bone marrow
Authors: Sanz-Ortega Laura , Andersson Agneta , Carlsten Mattias 
Published: January 2024

AbstractIncreased bone marrow (BM) homing of NK cells is associated with positive outcome in patients with acute myeloid leukemia (AML) treated within adoptive NK cell transfer trials. While most efforts to further improve the efficacy focus on augmenting NK cell persistence and cytotoxicity, few address their ability to home to the tumor. Here, we decipher how AML growth alters the BM niche to impair NK cell infiltration and how insights can be utilized to resolve this issue. We show that AML development gradually impairs the BM homing capacity of infused NK cells, which was tightly linked to loss of SDF-1α in this environment. AML development also triggered up-regulation of E-selectin on BM endothelial cells. Given the poor E-selectin-binding capacity of NK cells, introduction of fucosyltransferase-7 (FUT7) to the NK cells per mRNA transfection resulted in potent E-selectin binding and stronger adhesion to E-selectin+ endothelial cells. Co-introduction of FUT7 and gain-of-function CXCR4 (CXCR4R334X) redirected NK cell homing to the BM of AML-bearing mice nearly to the levels in AML-free mice. This work shows how impaired NK cell homing caused by AML-induced microenvironmental changes can be overcome by genetic engineering. We speculate our insights can help further advance future NK cell immunotherapies.
Ultrahigh-fidelity spatial mode quantum gates in high-dimensional space by diffractive deep neural networks
Authors: Wang Qianke , Liu Jun , Lyu Dawei , Wang Jian 
Published: January 2024

AbstractWhile the spatial mode of photons is widely used in quantum cryptography, its potential for quantum computation remains largely unexplored. Here, we showcase the use of the multi-dimensional spatial mode of photons to construct a series of high-dimensional quantum gates, achieved through the use of diffractive deep neural networks (D2NNs). Notably, our gates demonstrate high fidelity of up to 99.6(2)%, as characterized by quantum process tomography. Our experimental implementation of these gates involves a programmable array of phase layers in a compact and scalable device, capable of performing complex operations or even quantum circuits. We also demonstrate the efficacy of the D2NN gates by successfully implementing the Deutsch algorithm and propose an intelligent deployment protocol that involves self-configuration and self-optimization. Moreover, we conduct a comparative analysis of the D2NN gate’s performance to the wave-front matching approach. Overall, our work opens a door for designing specific quantum gates using deep learning, with the potential for reliable execution of quantum computation.
Highly efficient and aberration-free off-plane grating spectrometer and monochromator for EUV—soft X-ray applications
Authors: Li Jie , Li Kui , Zhang Xiaoshi , Popmintchev Dimitar , Xu Hao , Wang Yutong , Li Ruixuan , Zhang Guangyin , Tang Jiyue , Niu Jin , Ma Yongjun , Meng Runyu , Ke Changjun , Qiu Jisi , Ma Yunfeng , Popmintchev Tenio , Fan Zhongwei 
Published: January 2024

AbstractWe demonstrate a novel flat-field, dual-optic imaging EUV—soft X-ray spectrometer and monochromator that attains an unprecedented throughput efficiency exceeding 60% by design, along with a superb spectral resolution of λ/Δλ > 200 accomplished without employing variable line spacing gratings. Exploiting the benefits of the conical diffraction geometry, the optical system is globally optimized in multidimensional parameter space to guarantee optimal imaging performance over a broad spectral range while maintaining circular and elliptical polarization states at the first, second, and third diffraction orders. Moreover, our analysis indicates minimal temporal dispersion, with pulse broadening confined within 80 fs tail-to-tail and an FWHM value of 29 fs, which enables ultrafast spectroscopic and pump-probe studies with femtosecond accuracy. Furthermore, the spectrometer can be effortlessly transformed into a monochromator spanning the EUV—soft X-ray spectral region using a single grating with an aberration-free spatial profile. Such capability allows coherent diffractive imaging applications to be conducted with highly monochromatic light in a broad spectral range and extended to the soft X-ray region with minimal photon loss, thus facilitating state-of-the-art imaging of intricate nano- and bio-systems, with a significantly enhanced spatiotemporal resolution, down to the nanometer–femtosecond level.
Tissue factor overexpression promotes resistance to KRAS-G12C inhibition in non-small cell lung cancer
Authors: Zhang Yu , Liu Liang , Pei Jinpeng , Ren Zhiqiang , Deng Yan , Yu Ker 
Published: January 2024

AbstractThe recently approved KRASG12C mutation-specific inhibitors sotorasib and adagrasib (KRASG12C-I) represent a promising therapy for KRASG12C-driven non-small cell lung cancer (NSCLC). However, many eligible patients do not benefit due to intrinsic or acquired drug resistance. Tissue factor (TF) is overexpressed in KRAS-mutated (KRASmut) NSCLC and is the target of the FDA-approved ADC Tivdak. Here, we employed HuSC1-39, the parent antibody of a clinical stage TF-ADC (NCT04843709), to investigate the role of TF in KRASmut NSCLC. We found that patients with TF-overexpression had poor survival, elevated P-ERK/P-AKT activity levels and low immune effector cell infiltration in the tumor. In a panel of KRASG12C cell lines, KRASG12C-I response correlated with suppression of TF mRNA, which was not observed in resistant cells. In the drug resistant cells, TF-overexpression relied on an mTORC2-mediated and proteasome-dependent pathway. Combination treatment of HuSC1-39 or mTORC1/2 inhibitor MTI-31 with KRASG12C-I each produced synergistic antitumor efficacy in cell culture and in an orthotopic lung tumor model. TF-depletion in the resistant cells diminished epithelial mesenchymal transition, reduced tumor growth and greatly sensitized KRASG12C-I response. Moreover, employing immunohistochemistry and coculture studies, we demonstrated that HuSC1-39 or MTI-31 reset the tumor microenvironment and restore KRASG12C-I sensitivity by reshaping an M1-like macrophage profile with greatly enhanced phagocytic capacity toward tumor cell killing. Thus, we have identified the TF/mTORC2 axis as a critical new mechanism for triggering immunosuppression and KRASG12C-I resistance. We propose that targeting this axis with HuSC1-39 or MTI-31 will improve KRASG12C-I response in KRAS-driven NSCLC.
HPV-associated oropharyngeal cancer: in search of surrogate biomarkers for early lesions
Authors: Lim Yvonne X. , D’Silva Nisha J. 
Published: January 2024

AbstractThe incidence of oropharyngeal cancer (OPSCC) has escalated in the past few decades; this has largely been triggered by high-risk human papillomavirus (HPV). Early cancer screening is needed for timely clinical intervention and may reduce mortality and morbidity, but the lack of knowledge about premalignant lesions for OPSCC poses a significant challenge to early detection. Biomarkers that identify individuals at high risk for OPSCC may act as surrogate markers for precancer but these are limited as only a few studies decipher the multistep progression from HPV infection to OPSCC development. Here, we summarize the current literature describing the multistep progression from oral HPV infection, persistence, and tumor development in the oropharynx. We also examine key challenges that hinder the identification of premalignant lesions in the oropharynx and discuss potential biomarkers for oropharyngeal precancer. Finally, we evaluate novel strategies to improve investigations of the biological process that drives oral HPV persistence and OPSCC, highlighting new developments in the establishment of a genetic progression model for HPV + OPSCC and in vivo models that mimic HPV + OPSCC pathogenesis.
SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair
Authors: Huang Yujia , Tang Ming , Hu Zhiyi , Cai Bailian , Chen Guofang , Jiang Lijun , Xia Yan , Guan Pujun , Li Xiaoqi , Mao Zhiyong , Wan Xiaoping , Lu Wen 
Published: January 2024

AbstractEndometrial cancer (EC) stands as one of the most prevalent malignancies affecting the female genital tract, witnessing a rapid surge in incidence globally. Despite the well-established association of histone methyltransferase SMYD3 with the development and progression of various cancers, its specific oncogenic role in endometrial cancer remains unexplored. In the present study, we report that the expression level of SMYD3 is significantly upregulated in EC samples and associated with EC progression. Through meticulous in vivo and in vitro experiments, we reveal that depletion of SMYD3 curtails cell proliferation, migration, and invasion capabilities, leading to compromised non-homologous end joining repair (NHEJ) and heightened sensitivity of EC cells to radiation. Furthermore, our pathway enrichment analysis underscores the pivotal involvement of the DNA damage repair pathway in regulating EC progression. Mechanistically, in response to DNA damage, SMYD3 is recruited to these sites in a PARP1-dependent manner, specifically methylating LIG4. This methylation sets off a sequential assembly of the LIG4/XRCC4/XLF complex, actively participating in the NHEJ pathway and thereby fostering EC progression. Notably, our findings highlight the promise of SMYD3 as a crucial player in NHEJ repair and its direct correlation with EC progression. Intriguingly, pharmacological intervention targeting SMYD3 with its specific inhibitor, BCI-121, emerges as a potent strategy, markedly suppressing the tumorigenicity of EC cells and significantly enhancing the efficacy of radiotherapy. Collectively, our comprehensive data position SMYD3 as a central factor in NHEJ repair and underscore its potential as a promising pharmacological target for endometrial cancer therapy, validated through both in vitro and in vivo systems.