bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of nares swab to de-escalate vancomycin for patients with suspected methicillin-resistant Staphylococcus aureus
Authors: Gentges Joshua , El-Kouri Nadeem , Rahman Tashrique , Mushtaq Nasir , Hudson Ed , Scheck David 
Published: October 2023

Abstract

Introduction:
According to the US Center for Disease Control and Prevention, 30%–50% of antibiotic use in hospitals is unnecessary or inappropriate. The coronavirus disease 2019 pandemic further complicates antibiotic use leading to greater initiation of empiric antibiotics. The result is antibiotic overuse and increased duration of unnecessary therapy. Vancomycin is a drug of last resort, primarily relegated to the treatment of Methicillin-Resistant Staphylococcus aureus (MRSA). De-escalating vancomycin can mean waiting on MRSA culture results, which may take up to 96 h. Nares screening for MRSA is shown to possess high negative predictive value for ruling out suspected MRSA pneumonia, intra-abdominal infections, and bacteremia.

Methods:
This before-and-after study examines the impact of vancomycin therapy de-escalation due to absence of MRSA colonization detected via PCR assay of nares swabs. An intervention with providers using SMART goals was designed to increase nasal swabbing for MRSA and ultimately decrease vancomycin use at a large, tertiary-care urban hospital.

Results:
There was a significant increase in use of vancomycin nares swabs (28/150 vs 48/100, p = 0.040) in the immediate pre/postintervention period, and significant decreases in vancomycin usage days/1,000 patient days of 2.34% per month (p = 0.039) over a two year period after the intervention.

Conclusion:
An intervention using PCR nares swabs to detect MRSA led to significant, lasting decreases in vancomycin usage at this hospital. Similar interventions should be planned at hospitals experiencing overuse of this antibiotic.
Applications of electrothermal bipolar vessel sealing devices in transoral head and neck surgery
Authors: Chandrasiri Scott , Sahota Raguwinder Bindy Singh , Krishnan Suren , Foreman Andrew , Bassiouni Ahmed , Jervis-Bardy Jake , Hodge J C 
Published: October 2023

AbstractObjectiveElectrothermal bipolar vessel sealing devices are electrosurgical devices that seal tissues and blood vessels up to 7 mm in diameter. This paper discusses our experience using electrothermal bipolar vessel sealing devices in transoral head and neck surgery.MethodsElectrothermal bipolar vessel sealing devices were used in five patients with lesions of varying size and type within the pharyngo-larynx. These were treated transorally by the otolaryngology department at the Royal Adelaide Hospital. Either the Medtronic LigaSure or BiZact devices were used for transoral resection, debulking or division of these lesions.ResultsElectrothermal bipolar vessel sealing devices were considered helpful in four out of the five cases. Success was dependent on suitable transoral access. A single unsuccessful case reflected the LigaSure jaw's inability to engage a large tumour effectively.ConclusionElectrothermal bipolar vessel sealing devices can be safely deployed transorally to treat lesions of the upper aero-digestive tract in selected patients. Further studies investigating additional indications would broaden applications of this approach.
Armenian (Yerevan Eastern Armenian and Beirut Western Armenian)
Authors: Seyfarth Scott , Dolatian Hossep , Guekguezian Peter , Kelly Niamh , Toparlak Tabita 
Published: October 2023

Armenian (or , /hɑjeˈɾen/, ISO 639-1 hy) comprises an independent branch of the Indo-European language family.1 Its earliest attested ancestor is Classical Armenian in the fifth century CE (see Godel 1975; Thomson 1989; DeLisi 2015; Macak 2016). Modern Armenian is classified into two dialect families: Eastern Armenian (ISO 639-3 hye) and Western Armenian (ISO 639-3 hyw). Eastern Armenian is spoken in modern-day Armenia, and large speaker communities also exist in Georgia, Russia and Iran (shown in Figure 1). Western Armenian was historically spoken in the Ottoman Empire, but now includes varieties spoken throughout the Armenian diaspora in the Middle East, Europe, and the Americas (Donabédian 2018). There are substantial Western Armenian speaker communities in Turkey (Istanbul), Lebanon (Beirut), Syria (Aleppo, Damascus), California (Fresno, Los Angeles County), France (Marseilles), Australia (Sydney) and Argentina (Buenos Aires). There are also recent diaspora communities of Eastern Armenian speakers in California (Karapetian 2014), as well as communities of Western Armenian speakers in Armenia who escaped the Armenian genocide during World War I, who repatriated after World War II, or who fled the ongoing Syrian civil war. UNESCO lists Western Armenian as an endangered language in Turkey, and there are significant language promotion efforts in many diaspora communities that are intended to combat declining use by speaker generations born in the Americas and Europe (Al-Bataineh 2015; Chahinian & Bakalian 2016).
Exploring financial abuse involving people with dementia: an empirical legal study of nation-wide court rulings over a decade in Taiwan
Authors: Ku Li-Jung Elizabeth , Huang Sieh-Chuen , Liao Yi-Han , Kang Hsin-Yu , Chung Chao-An 
Published: October 2023

Abstract
Previous literature attempted to gain insight into financial abuse involving people with dementia by analysing court cases, but these studies were limited in sample size or scope. This study collected 214 court rulings directly related to the financial decisions of people with dementia to identify characteristics of the financial abuse victim, perpetrators and the types of assets. The models of bystander intervention and routine activity theory were used as conceptual models to guide analysis regarding the role of bank staff as well as the court's decision in cases of financial abuse. The majority of financial abuse perpetrators were family members (73.8%), as opposed to outsiders (19.2%). Transfer of real estate was the most common legal issue, and land was the most common financial asset involved. Difficult intra-family relationships seem to pose a great risk of financial abuse involving people with dementia since adult children were found to be the most likely perpetrators (52.7%) but also plaintiffs accusing financial abuse (57.6%). In accordance with the bystander intervention model, bank staff were more likely to be suspicious of financial abuse when an outsider was regarded as the perpetrator. In accordance with the routine activity theory model, the court was more likely to acknowledge the case as an invalid financial decision when an outsider was regarded as the perpetrator in financial abuse cases. Since people with dementia suffer from greater losses due to their family members, future policies should establish guidelines for front-line bank staff to identify warning signs to reduce the risk of financial abuse involving people with dementia, not only to prevent fraud by outsiders but also exploitation by family members.
A type-directed, dictionary-passing translation of method overloading and structural subtyping in Featherweight Generic Go
Authors: SULZMANN MARTIN , WEHR STEFAN 
Published: October 2023

Abstract
Featherweight Generic Go (FGG) is a minimal core calculus modeling the essential features of the programming language Go. It includes support for overloaded methods, interface types, structural subtyping, and generics. The most straightforward semantic description of the dynamic behavior of FGG programs is to resolve method calls based on runtime type information of the receiver. This article shows a different approach by defining a type-directed translation from

${\textrm{FGG}^{-}}$

to an untyped lambda-calculus.

${\textrm{FGG}^{-}}$

includes all features of FGG but type assertions. The translation of an

${\textrm{FGG}^{-}}$

program provides evidence for the availability of methods as additional dictionary parameters, similar to the dictionary-passing approach known from Haskell type classes. Then, method calls can be resolved by a simple lookup of the method definition in the dictionary. Every program in the image of the translation has the same dynamic semantics as its source

${\textrm{FGG}^{-}}$

program. The proof of this result is based on a syntactic, step-indexed logical relation. The step index ensures a well-founded definition of the relation in the presence of recursive interface types and recursive methods. Although being non-deterministic, the translation is coherent.
From cakravartin to bodhisattva: Buddhist models for globalization
Authors: Cohen Signe 
Published: October 2023

AbstractThis article examines globalization in an Asian context through the lens of two Buddhist concepts: the cakravartin and the bodhisattva. A cakravartin is a ruler who fuses spiritual and political power in his global reign. This article argues that the cakravartin represents one model of Buddhist globalization where the spread of the religion coincides with the growing military dominion of a BuddhGist king. A bodhisattva, on the other hand, is an enlightened being who has chosen to be reborn out of compassion with the entire suffering world. A bodhisattva watches over a ‘Buddha field’, or spiritual realm. Each Buddha field has its own laws, culture, language, or even separate forms of time and space. The bodhisattva provides a new model for understanding cultural diversity in the absence of a unified political power: the Buddhist world is a transnational network where new identities are negotiated.
Crystal dissolution by particle detachment
Authors: Zhu Guomin , Legg Benjamin A. , Sassi Michel , Liang Xinran , Zong Meirong , Rosso Kevin M. , De Yoreo James J. 
Published: October 2023

AbstractCrystal dissolution, which is a fundamental process in both natural and technological settings, has been predominately viewed as a process of ion-by-ion detachment into a surrounding solvent. Here we report a mechanism of dissolution by particle detachment (DPD) that dominates in mesocrystals formed via crystallization by particle attachment (CPA). Using liquid phase electron microscopy to directly observe dissolution of hematite crystals — both compact rhombohedra and mesocrystals of coaligned nanoparticles — we find that the mesocrystals evolve into branched structures, which disintegrate as individual sub-particles detach. The resulting dissolution rates far exceed those for equivalent masses of compact single crystals. Applying a numerical generalization of the Gibbs-Thomson effect, we show that the physical drivers of DPD are curvature and strain inherently tied to the original CPA process. Based on the generality of the model, we anticipate that DPD is widespread for both natural minerals and synthetic crystals formed via CPA.
GLP-1R signaling neighborhoods associate with the susceptibility to adverse drug reactions of incretin mimetics
Authors: Wright Shane C. , Motso Aikaterini , Koutsilieri Stefania , Beusch Christian M. , Sabatier Pierre , Berghella Alessandro , Blondel-Tepaz Élodie , Mangenot Kimberley , Pittarokoilis Ioannis , Sismanoglou Despoina-Christina , Le Gouill Christian , Olsen Jesper V. , Zubarev Roman A. , Lambert Nevin A. , Hauser Alexander S. , Bouvier Michel , Lauschke Volker M. 
Published: October 2023

AbstractG protein-coupled receptors are important drug targets that engage and activate signaling transducers in multiple cellular compartments. Delineating therapeutic signaling from signaling associated with adverse events is an important step towards rational drug design. The glucagon-like peptide-1 receptor (GLP-1R) is a validated target for the treatment of diabetes and obesity, but drugs that target this receptor are a frequent cause of adverse events. Using recently developed biosensors, we explored the ability of GLP-1R to activate 15 pathways in 4 cellular compartments and demonstrate that modifications aimed at improving the therapeutic potential of GLP-1R agonists greatly influence compound efficacy, potency, and safety in a pathway- and compartment-selective manner. These findings, together with comparative structure analysis, time-lapse microscopy, and phosphoproteomics, reveal unique signaling signatures for GLP-1R agonists at the level of receptor conformation, functional selectivity, and location bias, thus associating signaling neighborhoods with functionally distinct cellular outcomes and clinical consequences.
The Personalized Nutrition Study (POINTS): evaluation of a genetically informed weight loss approach, a Randomized Clinical Trial
Authors: Höchsmann Christoph , Yang Shengping , Ordovás José M. , Dorling James L. , Champagne Catherine M. , Apolzan John W. , Greenway Frank L. , Cardel Michelle I. , Foster Gary D. , Martin Corby K. 
Published: October 2023

AbstractWeight loss (WL) differences between isocaloric high-carbohydrate and high-fat diets are generally small; however, individual WL varies within diet groups. Genotype patterns may modify diet effects, with carbohydrate-responsive genotypes losing more weight on high-carbohydrate diets (and vice versa for fat-responsive genotypes). We investigated whether 12-week WL (kg, primary outcome) differs between genotype-concordant and genotype-discordant diets. In this 12-week single-center WL trial, 145 participants with overweight/obesity were identified a priori as fat-responders or carbohydrate-responders based on their combined genotypes at ten genetic variants and randomized to a high-fat (n = 73) or high-carbohydrate diet (n = 72), yielding 4 groups: (1) fat-responders receiving high-fat diet, (2) fat-responders receiving high-carbohydrate diet, (3) carbohydrate-responders receiving high-fat diet, (4) carbohydrate-responders receiving high-carbohydrate diet. Dietitians delivered the WL intervention via 12 weekly diet-specific small group sessions. Outcome assessors were blind to diet assignment and genotype patterns. We included 122 participants (54.4 [SD:13.2] years, BMI 34.9 [SD:5.1] kg/m2, 84% women) in the analyses. Twelve-week WL did not differ between the genotype-concordant (−5.3 kg [SD:1.0]) and genotype-discordant diets (−4.8 kg [SD:1.1]; adjusted difference: −0.6 kg [95% CI: −2.1,0.9], p = 0.50). With the current ability to genotype participants as fat- or carbohydrate-responders, evidence does not support greater WL on genotype-concordant diets. ClinicalTrials identifier: NCT04145466.
Induced photoelectron circular dichroism onto an achiral chromophore
Authors: Rouquet Etienne , Roy Chowdhury Madhusree , Garcia Gustavo A. , Nahon Laurent , Dupont Jennifer , Lepère Valéria , Le Barbu-Debus Katia , Zehnacker Anne 
Published: October 2023

AbstractAn achiral chromophore can acquire a chiral spectroscopic signature when interacting with a chiral environment. This so-called induced chirality is documented in electronic or vibrational circular dichroism, which arises from the coupling between electric and magnetic transition dipoles. Here, we demonstrate that a chiroptical response is also induced within the electric dipole approximation by observing the asymmetric scattering of a photoelectron ejected from an achiral chromophore in interaction with a chiral host. In a phenol–methyloxirane complex, removing an electron from an achiral aromatic π orbital localised on the phenol moiety results in an intense and opposite photoelectron circular dichroism (PECD) for the two enantiomeric complexes with (R) and (S) methyloxirane, evidencing the long-range effect (~5 Å) of the scattering chiral potential. This induced chirality has important structural and analytical implications, discussed here in the context of growing interest in laser-based PECD, for in situ, real time enantiomer determination.
Concretized structural evolution supported assembly-controlled film-forming kinetics in slot-die coated organic photovoltaics
Authors: Zhang Hao , Tian Chenyang , Zhang Ziqi , Xie Meiling , Zhang Jianqi , Zhu Lingyun , Wei Zhixiang 
Published: October 2023

AbstractBulk-heterojunction structured small-area organic solar cells are approaching 20% power conversion efficiency, but the blurred film-forming kinetics in the fabrication of large-area devices causes significant PCE loss and restrains the potential of commercialization. Such blurring came from insufficient knowledge of structural evolution during the film-forming process. Here, we concretize the evolution process with structures detailed to the submolecular level by comprehensive investigations of in-situ UV-vis spectroscopy, Atomic Force Microscope, Grazing Incident Wide Angle X-ray Scattering, and molecular dynamic simulation. With such hierarchical structural knowledge, assembly-controlled film-forming kinetics is proposed to explain the whole picture. Such assembly is determined by molecule configuration and can be tuned via external conditions. Understanding this kinetics will contribute to screening large-area device fabrication conditions, and the detailed structural knowledge could inspire the future design of novel photovoltaic materials that are intrinsically excellent in large-area device fabrications.
In vivo noninvasive systemic myography of acute systemic vasoactivity in female pregnant mice
Authors: Huda Kristie , Lawrence Dylan J. , Thompson Weylan , Lindsey Sarah H. , Bayer Carolyn L. 
Published: October 2023

AbstractAltered vasoactivity is a major characteristic of cardiovascular and oncological diseases, and many therapies are therefore targeted to the vasculature. Therapeutics which are selective for the diseased vasculature are ideal, but whole-body selectivity of a therapeutic is challenging to assess in practice. Vessel myography is used to determine the functional mechanisms and evaluate pharmacological responses of vascularly-targeted therapeutics. However, myography can only be performed on ex vivo sections of individual arteries. We have developed methods for implementation of spherical-view photoacoustic tomography for non-invasive and in vivo myography. Using photoacoustic tomography, we demonstrate the measurement of acute vascular reactivity in the systemic vasculature and the placenta of female pregnant mice in response to two vasodilators. Photoacoustic tomography simultaneously captures the significant acute vasodilation of major arteries and detects selective vasoactivity of the maternal-fetal vasculature. Photoacoustic tomography has the potential to provide invaluable preclinical information on vascular response that cannot be obtained by other established methods.
Asymmetric formal sp2-hydrocarbonations of dienes and alkynes via palladium hydride catalysis
Authors: Tang Ming-Qiao , Yang Zi-Jiang , He Zhi-Tao 
Published: October 2023

AbstractTransition metal-catalyzed asymmetric hydrofunctionalizations of unsaturated bonds via π-ƞ3 substitution have emerged as a reliable method to construct stereogenic centers, and mainly rely on the use of heteroatom-based or carbon nucleophiles bearing acidic C-H bonds. In comparison, sp2 carbon nucleophiles are generally not under consideration because of enormous challenges in cleaving corresponding inert sp2 C-H bonds. Here, we report a protocol to achieve asymmetric formal sp2 hydrocarbonations, including hydroalkenylation, hydroallenylation and hydroketenimination of both 1,3-dienes and alkynes via hydroalkylation and Wittig reaction cascade. A series of unachievable motifs via hydrofunctionalizations, such as di-, tri- and tetra-substituted alkenes, di-, tri- and tetra-substituted allenes, and tri-substituted ketenimines in allyl skeletons are all facilely constructed in high regio-, diastereo- and enantioselectivities with this cascade design. Stereodivergent synthesis of all four stereoisomers of 1,4-diene bearing a stereocenter and Z/E-controllable olefin unit highlights the power of present protocol. An interesting mechanistic feature is revealed that alkyne actually undergoes hydrocarbonation via the formation of conjugated diene intermediate, different from conventional viewpoint that the hydrofunctionalization of alkynes only involves allene species.
Lyophilization process optimization and molecular dynamics simulation of mRNA-LNPs for SARS-CoV-2 vaccine
Authors: Li Mingyuan , Jia Lin , Xie Yanbo , Ma Wenlin , Yan Zhihong , Liu Fufeng , Deng Jie , Zhu Ali , Siwei Xue , Su Wen , Liu Xiaofeng , Li Shiqin , Wang Haomeng , Yu Peng , Zhu Tao 
Published: October 2023

AbstractSome studies have shown that lyophilization significantly improves the stability of mRNA-LNPs and enables long-term storage at 2–8 °C. However, there is little research on the lyophilization process of mRNA-lipid nanoparticles (LNPs). Most previous studies have used empirical lyophilization with only a single lyoprotectant, resulting in low lyophilization efficiency, often requiring 40–100 h. In the present study, an efficient lyophilization method suitable for mRNA-LNPs was designed and optimized, shortening the total length of the lyophilization process to 8–18 h, which significantly reduced energy consumption and production costs. When the mixed lyoprotectant composed of sucrose, trehalose, and mannitol was added to mRNA-LNPs, the eutectic point and collapse temperature of the system were increased. The lyophilized product had a ginger root-shaped rigid structure with large porosity, which tolerated rapid temperature increases and efficiently removed water. In addition, the lyophilized mRNA-LNPs rapidly rehydrated and had good particle size distribution, encapsulation rate, and mRNA integrity. The lyophilized mRNA-LNPs were stable at 2–8 °C, and they did not reduce immunogenicity in vivo or in vitro. Molecular dynamics simulation was used to compare the phospholipid molecular layer with the lyoprotectant in aqueous and anhydrous environments to elucidate the mechanism of lyophilization to improve the stability of mRNA-LNPs. This efficient lyophilization platform significantly improves the accessibility of mRNA-LNPs.
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening
Authors: Porebski Benjamin T. , Balmforth Matthew , Browne Gareth , Riley Aidan , Jamali Kiarash , Fürst Maximillian J. L. J. , Velic Mirko , Buchanan Andrew , Minter Ralph , Vaughan Tristan , Holliger Philipp 
Published: October 2023

AbstractDeveloping therapeutic antibodies is laborious and costly. Here we report a method for antibody discovery that leverages the Illumina HiSeq platform to, within 3 days, screen in the order of 108 antibody–antigen interactions. The method, which we named ‘deep screening’, involves the clustering and sequencing of antibody libraries, the conversion of the DNA clusters into complementary RNA clusters covalently linked to the instrument’s flow-cell surface on the same location, the in situ translation of the clusters into antibodies tethered via ribosome display, and their screening via fluorescently labelled antigens. By using deep screening, we discovered low-nanomolar nanobodies to a model antigen using 4 × 106 unique variants from yeast-display-enriched libraries, and high-picomolar single-chain antibody fragment leads for human interleukin-7 directly from unselected synthetic repertoires. We also leveraged deep screening of a library of 2.4 × 105 sequences of the third complementarity-determining region of the heavy chain of an anti-human epidermal growth factor receptor 2 (HER2) antibody as input for a large language model that generated new single-chain antibody fragment sequences with higher affinity for HER2 than those in the original library.