bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fluorimetric, column‐switching HPLC and its application to an elimination study of LLU‐α enantiomers in rat plasma
Authors: Hattori Akihiro , Fukushima Takeshi , Hamamura Kimio , Kato Masaru , Imai Kazuhiro 
Published: August 2002

AbstractA method for the enantiomeric determination of 2,7,8‐trimethyl‐2‐(β‐carboxyethyl)‐6‐hydroxy chroman (LLU‐α, γ‐CEHC) in rat plasma was developed using high‐performance liquid chromatography (HPLC) with a fluorimetric derivatization with 4‐N, N‐dimethylaminosulfonyl‐7‐piperazino‐2,1,3‐benzoxadiazole (DBD‐PZ) followed by O‐acetylation with acetyl chloride. The proposed HPLC system used two non‐chiral columns (phenyl and octadecylsilica) and a chiral column (a modified cellulose type), which were connected via two column‐switching valves. A derivatized sample prepared from rat plasma was first separated on the phenyl column, and the fraction including LLU‐α derivative was introduced to the octadecylsilica column to quantify the concentration of the mixture of S‐ and R‐LLU‐α. Finally, the LLU‐α derivative was directly injected into the chiral column to obtain the ratio of the enantiomers. The proposed HPLC system was applied to the enantiomeric determination of LLU‐α in plasma after intravenous administration of racemic LLU‐α. S‐LLU‐α was eliminated faster than R‐LLU‐α, and its concentration in plasma decreased to one‐third at 2 min after dosing. Copyright © 2001 John Wiley & Sons, Ltd.
Abbreviations used:

DBD‐PZ

4‐N,N‐dimethylaminosulfonyl‐7‐piperazino‐2,1,3‐benzoxadiazole

DPDS

2,2′‐dipyridinyl disulfide

LLU‐α, γ‐CEHC

2,7,8‐trimethyl‐2‐(β‐carboxyethyl)‐6‐hydroxy chroman

TFA

trifluoroacetic acid

TPP

triphenylphosphine
Knowledge interests in corporate environmental management
Authors: Dobers Peter , Strannegård Lars , Wolff Rolf 
Published: August 2002

AbstractWe know from earlier studies that corporate environmental management is a young discipline, not yet integrated in general management and organization studies, but that researchers take an increasing part in the ongoing scientific conversation. However, the underlying knowledge interests characterizing the field of corporate environmental management is yet empirically unsubstantiated. One way to find out what elements make up the field is to analyse the contents of the most influential writings in the field. The present article identifies the 10 most cited works in Business Strategy and the Environment in 1992–2000 and explores the content of these texts. We conceptualise a typology for analysing corporate environmental management theory and formulate a characterization of the dominating knowledge interests. Our findings show that the theoretical fundament of corporate environmental management lacks a hermeneutic knowledge interest. Copyright © 2001 John Wiley & Sons, Ltd and ERP Environment
The evolution of environmental management: from stage models to performance evaluation
Authors: Kolk Ans , Mauser Anniek 
Published: August 2002

AbstractIn the past two decades, academics and practitioners have attempted to improve understanding of environmental management by classifying companies' environmental behaviour, and evaluating their performance. Driven by both research and societal interest, this has resulted in a wave of stage or phase models, and a range of typologies. This article gives an overview of the development of such environmental management models, analysing their characteristics, strengths and weaknesses. An evolution can be noted in the direction of typologies and non‐linear models to deal with organizational and strategic complexities. Models are starting to pay more attention to the management side. To overcome problems of operationalization and limited company and sector specificity, environmental performance evaluation systems have emerged more recently. Although comprehensive performance assessments are still unavailable, the tenets of such a system can already be delineated. The paper presents these components, and draws conclusions on the contribution of environmental management models and performance evaluation systems. Copyright © 2002 John Wiley & Sons, Ltd. and ERP Environment
Pretarsal fat compartment in the lower eyelid
Authors: Hwang Kun , Joong Kim Dae , Chung Robert S. 
Published: August 2002

AbstractIt is generally accepted that there are three infraorbital fat regions in the lower eyelid; medial, central, and lateral compartments. However, removing only the fat in the lateral compartment does not remove the bulge just below the eyelashes, which is caused by another fat pad. The aim of this study was to describe the anatomy of the pretarsal fat compartment and to demonstrate its clinical implications in lower lid blepharoplasty. Ten cadavers (total 20 lower eyelids) were studied. A skin‐muscle flap was reflected to expose the soft pretarsal structures. A small stab incision was made on the lateral portion of the sac containing fat on the tarsus. Methylene blue dye was injected into the sac. Specimens were fixed and sagittal sections in four different planes were prepared for histological analysis. The injected dye remained within the sac and demarcated it as a pear or cone shaped structure. This encapsulated fat compartment sits on the lateral half of the tarsal plate above the lateral compartment fat. Auxillary or submuscular fat is well known. This study, however, designates the pretarsal fat as “encapsulated” in a compartment instead of being unbound. We have named it the “pretarsal fat compartment.” Histologically, orbital septal fibers separate “pretarsal fat” from lateral infraorbital fat. It is recommended that fat in the pretarsal fat compartment be removed during lower lid blepharoplasty in order to alleviate the bulge or knoll of the skin just below the lower eyelashes. Clin. Anat. 14:179–183, 2001. © 2001 Wiley‐Liss, Inc.
Eicosapentaenoic acid and docosahexaenoic acid effects on tumour mitochondrial metabolism, acyl CoA metabolism and cell proliferation
Authors: Colquhoun Alison , Ramos Karina L. , Schumacher Robert I. 
Published: August 2002

AbstractIn order to investigate the effects of high‐fat diets rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), Wistar rats bearing subcutaneous implants of the Walker 256 tumour were fed pelleted chow containing low DHA/EPA or high DHA/EPA. The presence of n‐3 polyunsaturated fatty acids (PUFAs) led to a marked suppression (35–46%) of tumour growth over a 12 day period. Both the whole tumour homogenate and the Percoll‐purified mitochondrial fraction presented significant changes in fatty acid composition. The levels of EPA increased in both n‐3 dietary groups while the levels of DHA increased only in the high DHA/EPA group, in comparison with the control chow‐fed group. The presence of n‐3 PUFAs led to an increase in mitochondrial acyl CoA synthetase activity, but neither the cytoplasmic acyl CoA content nor the n‐3 fatty acid composition of the cytoplasmic acyl CoAs was altered by the diet. The content of thiobarbituric acid‐reactive substances (TBARS) was increased in the low DHA/EPA group but was unchanged in the high DHA/EPA group. In vitro studies with the Walker 256 cell line showed a 46% decrease in cell growth in the presence of either EPA or DHA which was accompanied by a large decrease in the measured mitochondrial membrane potential. The TBARS content was increased only in the EPA‐exposed cells. Cell cycle analysis identified a decrease in G0–G1 phase cells and an increase in G2–M phase cells and apoptotic cells, for both EPA and DHA‐exposed cells. The data show that the presence of n‐3 PUFAs in the diet is able to significantly after the growth rate of the Walker 256 tumour. The involvement of changes in mitochondrial membrane composition and membrane potential have been indicated for both EPA and DHA, while changes in lipid peroxidation have been identified in the presence of EPA but not of DHA. Copyright © 2001 John Wiley & Sons, Ltd.
The uncoupling effect of the nonsteroidal anti‐inflammatory drug nimesulide in liver mitochondria from adjuvant‐induced arthritic rats
Authors: Caparroz‐Assef S. M. , Salgueiro‐Pagadigorria C. L. , Bersani‐Amado C. A. , Bracht A. , Kelmer‐Bracht A. M. , Ishii‐Iwamoto E. L. 
Published: August 2002

AbstractThe aim of the present study was to evaluate the changes caused by adjuvant‐induced arthritis in liver mitochondria and to investigate the effects of the nonsteroidal anti‐inflammatory drug nimesulide. The main alterations observed in liver mitochondria from arthritic rats were: higher rates of state IV and state III respiration with β‐hydroxybutyrate as substrate; reduced respiratory control ratio and impaired capacity for swelling dependent on β‐hydroxybutyrate oxidation. No alterations were found in the activities of NADH oxidase and ATPase. Nimesulide produced: (1) stimulation of state IV respiration; (2) decrease in the ADP/O ratio and in the respiratory control ratio; (3) stimulation of ATPase activity of intact mitochondria; (4) inhibition of swelling driven by the oxidation of β‐hydroxybutyrate; (5) induction of passive swelling due to NH3/NH4+ redistribution. The activity of NADH oxidase was insensitive to nimesulide. Mitochondria from arthritic rats showed higher sensitivity to nimesulide regarding respiratory activity. The results of this work allow us to conclude that adjuvant‐induced arthritis leads to quantitative changes in some mitochondrial functions and in the sensitivity to nimesulide. Direct evidence that nimesulide acts as an uncoupler was also presented. Since nimesulide was active in liver mitochondria at therapeutic levels, the impairment of energy metabolism could lead to disturbances in the liver responses to inflammation, a fact that should be considered in therapeutic intervention. Copyright © 2001 John Wiley & Sons, Ltd.
Recanalization and thrombectomy of internal anastomosis in a patient with tetralogy of Fallot using the AngioJet rheolytic catheter
Authors: Kokov L.S. , Korostelev A.N. , Grinko A.N. , Ilyina M.V. , Solopova G.V. , Aiazian E. , Setum C.M. 
Published: August 2002

AbstractThe modified Blalock‐Taussig shunt is the palliative treatment of choice for tetralogy of Fallot. Shunt thrombosis is a potential complication, requiring high‐risk reoperation. The use of percutaneous rheolytic devices for thrombus removal in such occluded shunts has not been previously reported. We describe a case in which use of a rheolytic catheter resulted in significant thrombus removal and rapid reversal of cyanosis and dyspnea in a 5‐year‐old patient. The patient remains free of symptoms at 30‐day follow‐up. Cathet Cardiovasc Intervent 2001;53:504–507. © 2001 Wiley‐Liss, Inc.
Intra‐aortic counterpulsation during carotid stenting
Authors: Hennen Benno , Gröschel‐Guth Angelika , Scheller Bruno , Hammer Bernd , Böhm Michael 
Published: August 2002

AbstractPostprocedural hypotension following endovascular stent placement of carotid artery disease (CAS) predicts increased in‐hospital complications and long‐term risk of death. Intra‐aortic balloon counterpulsation (IABP) both increases mean arterial pressure and cerebral blood flow and therefore possibly reduces complications due to hemodynamic instability during and after CAS. In this study, we describe the use of IABP in a patient with severe depression of left ventricular function due to diffuse coronary artery disease undergoing CAS. Controlled studies are necessary to demonstrate a potentially protective role of IABP in high‐risk CAS patients. Cathet Cardiovasc Intervent 2001;53:546–548. © 2001 Wiley‐Liss, Inc.
The patentability of biomolecules – does online bioinformatics compromise novelty?
Authors: Hutter Anton 
Published: August 2002

AbstractResearchers are becoming increasingly concerned that the confidentiality
of their novel biomolecule sequences is being jeopardised, particularly when
these sequences are either submitted to sequence databases or uploaded as
query terms onto internet‐based bioinformatic software suites. The researcher's
fears stem from the fact that the actual uploading of their sequences acts
as a novelty destroying prior disclosure or publication, and that this may
subsequently preclude valid patent protection for the sequences. This article
addresses the key issues involved in the analyses of biomolecules, highlighting
potential risks taken by many researchers in regard to patent protection and
suggests possible ways in which these risks may be mitigated. Copyright ©
2002 John Wiley & Sons, Ltd.
Featured Organism: Arabidopsis thaliana
Authors: Wixon Jo 
Published: August 2002

AbstractArabidopsis is universally acknowledged as the model for dicotyledonous crop plants. Furthermore, some of the information gleaned from this small plant can be used to aid work on monocotyledonous crops. Here we provide an overview of the current state of knowledge and resources for the study of this important model plant, with comments on future prospects in the field from Professor Pamela Green and Dr Sean May. Copyright © 2001 John Wiley & Sons, Ltd.Summary

•  Small flowering plant of mustard family (Brassicaceae)
•  Rapid life cycle, high seed production, easy to cultivate
•  Small genome, 125 Mb, as five chromosomes
•  Genome sequence completed, 25,498 genes identified
•  30% of genes currently have no functional classification
•  Majority of genome present in two copies, suggesting whole genome duplication
Optical resolution of 6‐fluoro‐2‐methyl‐1,2,3,4‐tetrahydroquinoline by supercritical fluid extraction
Authors: Kmecz Ildikó , Simándi Béla , Bálint József , Székely Edit , Fogassy Elemér , Kemény Sándor 
Published: August 2002

Abstract6‐Fluoro‐2‐methyl‐1,2,3,4‐tetrahydroquinoline (FTHQ) enantiomers were separated by supercritical fluid extraction using carbon dioxide. Diastereoisomeric salts were formed from the racemic base with less than one equivalent of O,O′‐di‐(4‐toluoyl)‐(2R,3R)‐tartaric acid (DPTTA). Further purification was achieved by partial salt formation of the enantiomeric mixture with an achiral acid (HCl) followed by the supercritical fluid extraction of the free enantiomers. Chirality 13:568–570, 2001. © 2001 Wiley‐Liss, Inc.
Enantiomeric recognition of chiral 3,3‐bridged‐1,1′‐binaphthol dimer toward α‐phenylethylamine and α‐amino acid ester
Authors: Liu Tian Jun , Chen Yong Jun , Zhang Ke Sheng , Wang Dong , Guo Da Wei , Yang Xiao Zhen 
Published: August 2002

AbstractThe 1,1′‐binaphthol‐based dimers with p‐phenylenebis(2‐ethynyl) spacer, (+)‐6 and (+)‐2, were synthesized as chiral host compounds. 1H NMR, UV‐vis, and fluorescent titration were used to evaluate the enantiomeric recognition abilities of the chiral host dimers toward the guest amine 7 and α‐amino acid ester 8. The chiral BINOL‐based dimers were found to have good enantiomeric recognition ability. The computer simulation of the host–guest complex molecules was carried out to describe the conformational changes of both naphthyl ring in the molecule of chiral host dimer after complexation with the guest molecule. Chirality 13:595–600, 2001. © 2001 Wiley‐Liss, Inc.
Reorganization of the actin cytoskeleton in the protruding lamellae of human fibroblasts
Authors: Safiejko‐Mroczka Barbara , Bell Paul B. 
Published: August 2002

AbstractTo investigate the mechanisms of protrusion in vertebrate cells, the primary event in cell motility, human fibroblasts were treated with neomycin, an inhibitor of the phosphatidylinositol cycle, to induce protrusion. Changes in cell motility and the cytoskeleton were examined by video, fluorescence, scanning electron, and confocal microscopy and by cytofluorometry. Protrusion in neomycin‐treated human fibroblasts is correlated with a transient overall decrease in F‐actin followed by an increase in F‐actin at the leading edge of the protruding lamella. In growing lamellae, F‐actin is organized in a marginal band at the leading edge. Although actin is present in the lamella behind the leading edge, very little of it is F‐actin. Scanning electron microscopy of detergent‐extracted cells reveals a band of dense filaments at the leading edge, corresponding to the marginal band of F‐actin seen in fluorescently labeled cells, and a sparse population of short, fragmented filaments, in the rest of the lamella. Gelsolin is colocalized with F‐actin in the marginal band and is also present in the lamella where F‐actin is largely absent. The data support the hypothesis that the protrusion is initiated by the breakdown of cortical actin filaments, possibly mediated by gelsolin, whereas expansion of the protrusion requires de novo polymerization of actin filaments at the leading edge. Cell Motil. Cytoskeleton 50:13–32, 2001. © 2001 Wiley‐Liss, Inc.
Magnetic resonance imaging compatible neonate incubator
Authors: Dumoulin Charles L. , Rohling Kenneth W. , Piel Joseph E. , Rossi Charles J. , Giaquinto Randy O. , Watkins Ronald D. , Vigneron Daniel B. , Barkovich A. James , Newton Nancy 
Published: August 2002

AbstractAn incubator for magnetic resonance imaging (MRI) of premature and full‐term infants was designed and constructed. The device is an extensively modified MRI system table that has a permanently attached incubator housing, self‐contained power supply, and full patient monitoring capability. A custom eight‐element birdcage coil within the incubator slides over the infant and provides high sensitivity for MRI. The incubator provides all the temperature control and patient monitoring found in conventional neonatal intensive care incubators, but in an MRI‐compatible fashion. The table can operate for at least three hours on a single battery charge. It is believed that this table design will enable routine MRI scanning of newborn infants requiring extensive monitoring of vital signs and/or temperature regulation. © 2002 Wiley Periodicals, Inc. Concepts in Magnetic Resonance (Magn Reson Engineering) 15: 117–128, 2002
HER‐2/neu overexpression is rare in hepatocellular carcinoma and not predictive of anti‐HER‐2/neu regulation of cell growth and chemosensitivity
Authors: Hsu Chiun , Huang Chin‐Lun , Hsu Hey‐Chi , Lee Po‐Huang , Wang Shiou‐Jeng , Cheng Ann‐Lii 
Published: August 2002

AbstractBACKGROUNDThe overexpression of HER‐2/neu oncogene has been implicated in the development and modulation of many types of cancer. However, whether HER‐2/neu overexpression plays a similar role in hepatocellular carcinoma (HCC) has not been determined.METHODSTissue specimens from 36 HCC patients who had been enrolled in 3 separate prospective clinical trials of systemic chemotherapy were studied by immunohistochemical staining. A polyclonal antibody (A0485; DAKO, Copenhagen, Denmark) against HER‐2/neu and a horseradish peroxidase‐based visualization system (Envision+, DAKO) was used. Scoring criteria was in accordance with the manufacturer's guidelines. Twelve HCC cell lines were examined for HER‐2/neu overexpression by Western blotting. Single‐agent growth regulatory activity of the anti–HER‐2/neu antibody, trastuzumab (Herceptin; Genentech, South San Francisco, CA), and its combinative cytotoxicity with chemotherapeutic agents (doxorubicin, gemcitabine, cisplatin, irinotecan) were determined by a tetrazolium‐based colorimetric assay (MTT test).RESULTSAll but one of the HCC tumor tissues were negative for HER‐2/neu expression. The only patient with positive HER‐2/neu expression was a 57‐year‐old male patient who achieved stabilization of disease for 2 months after chemotherapy. Eight of the 35 patients with negative HER‐2/neu expression had had partial remission after chemotherapy (P = 0.78). Only one (Tong cells) of the 12 HCC cell lines had a significant level of HER‐2/neu expression. However, trastuzumab up to 10 μg/mL had no discernible growth inhibitory or chemosensitizing effect on Tong cells or any other cell lines.CONCLUSIONSHER‐2/neu overexpression is rare in human HCC tissues, and anti–HER‐2/neu regulation appears to play little role in the treatment of this tumor. Cancer 2002;94:415–20. © 2002 American Cancer Society.
European Organization for Research and Treatment of Cancer and Groupe d'Etude des Lymphomes de l'Adulte very favorable and favorable, lymphocyte‐predominant Hodgkin disease
Authors: Wilder Richard B. , Schlembach Pamela J. , Jones Dan , Chronowski Gregory M. , Ha Chul S. , Younes Anas , Hagemeister Fredrick B. , Barista Ibrahim , Cabanillas Fernando , Cox James D. 
Published: August 2002

AbstractBACKGROUNDLymphocyte‐predominant Hodgkin disease (LPHD) is rare and has a natural history different from that of classic Hodgkin disease. There is little information in the literature regarding the role of chemotherapy in patients with early‐stage LPHD. The objective of this study was to examine recurrence free survival (RFS), overall survival (OS), and patterns of first recurrence in patients with LPHD who were treated with radiotherapy alone or with chemotherapy followed by radiotherapy.METHODSFrom 1963 to 1996, 48 consecutive patients ages 16–49 years (median, 28 years) with Ann Arbor Stage I (n = 30 patients) or Stage II (n = 18 patients), very favorable (VF; n = 5 patients) or favorable (F; n = 43 patients) LPHD, according to the European Organization for Research and Treatment of Cancer and Groupe d'Etude des Lymphomes de l'Adulte (EORTC‐GELA) criteria, received radiotherapy alone (n = 37 patients) or received chemotherapy followed by radiotherapy (n = 11 patients). The percentages of patients with VF disease (11% vs. 9% in the radiotherapy group vs. the chemotherapy plus radiotherapy group, respectively) or F disease (89% vs. 91%, respectively) within the two treatment groups were similar (P = 1.00). A median of three cycles of chemotherapy with mechlorethamine, vincristine, procarbazine, and prednisone (MOPP) or with mitoxantrone, vincristine, vinblastine, and prednisone (NOVP) was given initially to six patients and five patients, respectively. A median total radiotherapy dose of 40 grays (Gy) given in daily fractions of 2.0 Gy was delivered to both treatment groups.RESULTSThe median follow‐up was 9.3 years, and 98% of patients were observed for ≥ 3.0 years. RFS was similar for patients who were treated with radiotherapy alone and patients who were treated with chemotherapy followed by radiotherapy (10‐year survival rates: 77% and 68%, respectively; P = 0.89). The OS rate also was similar for the two groups (10‐year survival rates: 90% and 100%, respectively; P = 0.43). MOPP or NOVP chemotherapy did not reduce the risk of recurrence outside of the radiotherapy fields.CONCLUSIONSMOPP or NOVP chemotherapy did not improve RFS or OS significantly in patients with VF or F LPHD, although the statistical power was limited. Ongoing clinical trials will help to clarify the role of a watch‐and‐wait strategy or systemic therapy, including anthracycline (epirubicin or doxorubicin), bleomycin, and vinblastine‐based chemotherapy or antibody‐based approaches, in the treatment of these patients. Cancer 2002;94:1731–8. © 2002 American Cancer Society.DOI 10.1002/cncr.10404