bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Potential Role of Pyrroloquinoline Quinone to Regulate Thyroid Function and Gut Microbiota Composition of Graves’ Disease in Mice
Authors: Liu Xiaoyan , Jiang Wen , Lu Ganghua , Qiao Tingting , Gao Dingwei , Zhang Mengyu , Cai Haidong , Chai Li , Yi Wanwan , Lv Zhongwei 
Published: December 2023

Abstract
Graves’ disease (GD) is an autoimmune disorder disease, and its prevalence continues to increase worldwide. Pyrroloquinoline quinone (PQQ) is a naturally antioxidant compound in milk, vegetables, and meat. We aim to identify the treatment efficacy of PQQ on GD and its regulatory effect on intestinal microbiota. The GD mice model was built by an adenovirus expressing autoantigen thyroid-stimulating hormone receptor (Ad-TSHR289). Fecal samples were collected for 16S rDNA sequencing after PQQ pretreatments (20, 40, or 60 mg/kg BW/day) for 4 weeks. Thyroid and intestine functions were measured. The levels of serum TSHR and T4 were significantly raised, and the thyroid gland size was typically enlarged in the GD group than in controls, reversed by PQQ therapy. After PQQ replenishment, IL6 and TNFα levels in small intestine tissues were lower than those in the GD group, with Nrf2 and HO1 levels improved. Also, the PQQ supplement could maintain the mucosal epithelial barrier impaired by GD. In microbial analyses, PQQ treatment could prompt the diversity recovery of gut microbiota and reconstruct the microbiota composition injured by GD. Lactobacillus served as the most abundant genus in all groups, and the abundance of Lactobacillus was increased in the GD group than in control and PQQ groups. Besides, Lactobacillus was highly correlative with all samples and the top 50 genera. PQQ supplementation regulates thyroid function and relieves intestine injury. PQQ changes the primary composition and abundance of GD’s intestine microbiota by moderating Lactobacillus, which may exert in the pathogenesis and progression of GD.
JENS PETER JAKOBSEN'S NOVELLA “MOGENS” TRANSLATED BY OLHA KOBYLIANSKA: TEXT AND CONTEXTS
Authors: Kyryliuk Svitlana 
Published: December 2023

The problem of Olha Kobylianska’s interrelationship with European literature has been in the field of researchers’ vision since the Ukrainian author’s appearance in literature. The emergence of new facts related to this problem can accentuate the writer’s conscious focus on mastering the cultural experience of other literatures. O. Kobylianska often emphasized such moments in her own works, naming the authors that influenced her. Among them is the Danish writer Jens Peter Jacobsen. Kobylianska translated Jacobsen’s short story “There Should Have Been Roses” and wrote her original work — a prose poetry “Roses”, containing some allusions and coincidences pointing to the Danish writer’s work. However, this was not the only point connecting the literary worlds of J. P. Yacobsen and O. Kobylianska.
The other fact of such creative ‘convergence’, unknown until now, is the Ukrainian translation of J. P. Jacobsen’s novella “Mogens” (the unfinished autograph translation is kept in the Manuscript Collections and Textual Studies Department of the Institute of Literature in the fund of Olha Kobylianska under the name “Morene” and with the note “Olha Kobylianska. Unfinished story” (DM ІL, fund 14, folder 1338). The present paper offers attribution of it, including the clarified title and approximate date. The Ukrainian text of the story has been prepared for publication. The inclusion of the incomplete Ukrainian translation of J. P. Jacobsen’s novella into scholarly discourse provides new insights for assessing peculiarities and stylistic parameters of O. Kobylianska’s prose of the late 19th and early 20th centuries. This fact also enhances our understanding of the historical connections between Ukrainian and European literatures, especially Danish literature. The research of O. Kobylianska and J. P. Jacobsen’s works is based on the principles of textual studies, historical-genetic approach, comparative studies, and analytical anthropology.
GÖRSEL FOLKLOR VE FOTOĞRAF
Authors: UĞURELİ Ayşe 
Published: December 2023

1839 yılında teknik olarak görüntüleri yüzey üzerinde sabitleyen daguerretype adlı icadın duyurulmasından yedi yıl sonra 1846’da folklor terimi ilk defa William John Thoms tarafından kullanılmıştır. İcat edildikten kısa bir süre sonra hayatın her alanında aktif bir şekilde kullanılan fotoğraf, bilim dünyasına da hızlı bir şekilde giriş yapmıştır. Özellikle sosyal bilimlerde/toplum bilimlerinde fotoğraf, bilimsel ilerleme anlamında önemli aşamalar kat edilmesine vesile olmuştur. Antropoloji, etnografya ve sosyolojide fotoğraf kullanımı, disiplinlerin ilk gelişim evrelerine denk gelmiş ve bir süre sonra da disiplinlerin alt dalları olarak görsel antropoloji, görsel etnografya ve görsel sosyoloji olarak kuramsallaşmıştır. Folklor ise bilimsel olarak kuramsallaşma döneminde genellikle antropoloji ve etnografyanın fotoğraf kullanma prensiplerini ödünçlemiştir. Bunun en önemli sebebi antropoloji ve etnografyada fotoğraf kullanma prensiplerinin kültürel çalışmalar için oldukça kapsamlı olmasıdır.
Konuyu folklor özelinde ele alacak olursak fotoğraf ve folklor, gelişim süreçlerinde omuz omuza yürümüştür. Birbirlerine yakın zamanlarda doğan, tarihi gelişim sürecini omuz omuza kat eden, ortak özellikleri hayatın her alanına nüfuz edebilmek olan bir icat ve bir bilim dalının birbirini etkilemesi, birbirleriyle (olumlu ya da olumsuz) temas etmesi tabii bir beklentidir. Özellikle folklorun bir bilim dalı olarak gelişim gösterdiği dönemde arşiv folklorculuğunun ön plana çıktığı düşünüldüğünde fotoğrafın folklora etkisini ilk dönemlerinden başlayarak tarihi gelişimi içerisinde incelemek ve günümüze kadar gelen süreci, folklorun gelişimine paralel bir şekilde ele almak önem arz etmektedir. Bu ihtiyaçlar doğrultusunda, bu makalede kısaca fotoğraf tarihi ele alındıktan sonra etnografya, antropoloji ve sosyolojinin fotoğraf kullanma prensiplerine yer verilmiştir. Bu prensipler görsel folklorun hazırlayıcıları olarak değerlendirilmiş ve bunlara binaen “Görsel folklor nedir?” sorusunun cevabı aranmış ve bir tanım denemesi yapılmıştır.
Factors associated with diabetes concordant comorbidities among adult diabetic patients in Central Ethiopia: a cross-sectional study
Authors: Negussie Yohannes Mekuria , Getahun Mihiret Shawel , Bekele Nardos Tilahun 
Published: December 2023

BackgroundDiabetes comorbidities are a serious public health issue that raises the risk of adverse health effects and complicates diabetes management. It also harms emotional health, medication adherence, self-management, and general quality of life. However, evidence is scarce in Ethiopia, particularly in the study area. Thus, this study aimed to estimate the prevalence of diabetes concordant comorbidities and identify factors associated with the presence of concordant comorbidities among adult diabetic patients in central Ethiopia.MethodsA health facility-based cross-sectional study was conducted among 398 adult diabetic patients. A computer-generated simple random sampling was used to select study participants. Data were collected using a structured data extraction checklist. The collected data were entered into Epi info version 7.2 and exported to SPSS version 27 for analysis. A binary logistic regression model was used to analyze the association between dependent and independent variables. An adjusted odds ratio with the corresponding 95% confidence interval was used to measure the strength of the association and statistical significance was declared at a p-value &lt; 0.05.ResultThe prevalence of diabetes-concordant comorbidities was 41% (95% CI: 36.2-46.0). The multivariable logistic regression model showed that age 41–60 (AOR = 2.86, 95% CI: 1.60–5.13), place of residence (AOR = 2.22, 95% CI: 1.33–3.70), having type two diabetes (AOR = 3.30, 95% CI: 1.21–8.99), and having positive proteinuria (AOR = 2.64, 95% CI: 1.47–4.76) were significantly associated with diabetes concordant comorbidities.ConclusionThe prevalence of diabetes-concordant comorbidities was relatively high. Age, place of residence, type of diabetes, and positive proteinuria were factors associated with diabetes-concordant comorbidities. Prevention, early identification, and proper management of diabetes comorbidities are crucial.
Identification of efferocytosis-related subtypes in gliomas and elucidating their characteristics and clinical significance
Authors: Gao Mengge , Huang Jinsheng , Yang Bo , Liu Qiong , Luo Miaoqing , Yang Biying , Li Xujia , Liu Xiaofang 
Published: December 2023

Introduction: Gliomas, the most prevalent tumors of the central nervous system, are known for their aggressive nature and poor prognosis. The heterogeneity among gliomas leads to varying responses to the same treatments, even among similar glioma types. In our study, we efferocytosis-related subtypes and explored their characteristics in terms of immune landscape, intercellular communication, and metabolic processes, ultimately elucidating their potential clinical implications.Methods and Results: We first identified efferocytosis-related subtypes in Bulk RNA-seq using the NMF algorithm. We then preliminarily demonstrated the correlation of these subtypes with efferocytosis by examining enrichment scores of cell death pathways, macrophage infiltration, and the expression of immune ligands. Our analysis of single-cell RNA-seq data further supported the association of these subtypes with efferocytosis. Through enrichment analysis, we found that efferocytosis-related subtypes differ from other types of gliomas in terms of immune landscape, intercellular communication, and substance metabolism. Moreover, we found that the efferocytosis-related classification is a prognostic factor with robust predictive performance by calculating the AUC values. We also found that efferocytosis-related subtypes, when compared with other gliomas in drug sensitivity, survival, and TIDE scores, show a clear link to the effectiveness of chemotherapy, radiotherapy, and immunotherapy in glioma patients.Discussion: We identified efferocytosis-related subtypes in gliomas by analyzing the expression of 137 efferocytosis-associated genes, exploring their characteristics in immune landscape, intercellular communication, metabolic processes, and genomic variations. Moreover, we discovered that the classification of efferocytosis-related subtypes has a strong prognostic predictive power and holds potential significance in guiding clinical treatment.
The genetic puzzle of a SOD1-patient with ocular ptosis and a motor neuron disease: a case report
Authors: Vacchiano Veria , Palombo Flavia , Ormanbekova Danara , Fiorini Claudio , Fiorentino Alessia , Caporali Leonardo , Mastrangelo Andrea , Valentino Maria Lucia , Capellari Sabina , Liguori Rocco , Carelli Valerio 
Published: December 2023

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a complex genetic architecture, showing monogenic, oligogenic, and polygenic inheritance. In this study, we describe the case of a 71 years-old man diagnosed with ALS with atypical clinical features consisting in progressive ocular ptosis and sensorineural deafness. Genetic analyses revealed two heterozygous variants, in the SOD1 (OMIM*147450) and the TBK1 (OMIM*604834) genes respectively, and furthermore mitochondrial DNA (mtDNA) sequencing identified the homoplasmic m.14484T&gt;C variant usually associated with Leber’s Hereditary Optic Neuropathy (LHON). We discuss how all these variants may synergically impinge on mitochondrial function, possibly contributing to the pathogenic mechanisms which might ultimately lead to the neurodegenerative process, shaping the clinical ALS phenotype enriched by adjunctive clinical features.
The role of pudendal nerve block in hemorrhoid surgery: a systematic review and meta-analysis of double-blind randomized controlled trials
Authors: Xia Shijun , Luo Lidan , Wu Wenjiang , Lu Kaiyuan , Jiang Tao , Li Yue 
Published: December 2023

BackgroundPudendal nerve block (PNB) is a commonly used anesthesia method that has been widely used in postoperative analgesia for hemorrhoids in recent years. Therefore, we conducted a systematic review and meta-analysis of double-blind randomized controlled trials (RCTs) to analyze the effectiveness of PNB in postoperative analgesia for hemorrhoids.MethodsRelevant data and studies published from inception until August 14, 2023, were retrieved from PubMed, Embase, and Web of Science to evaluate the beneficial effects of PNB for analgesia following hemorrhoidectomy.ResultsThis meta-analysis included 6 double-blind RCTs comprising 501 patients. We evaluated the function of PNB in improving outcomes of postoperative analgesia of hemorrhoids. Visual analogue scale (VAS) scores on postoperative within 6 h (MD, −3.04; 95% CI, −4.13 to −1.95; P &lt; 0.0001), 12 h (MD, −3.14; 95% CI, −3.87 to −2.40; P &lt; 0.0001), and 24 h (MD, −2.25; 95% CI, −2.95 to −1.55; P &lt; 0.0001) were enhanced by the application of PNB, but not in 48 h (MD, −2.54; 95% CI, −5.29 to 0.20; P = 0.07).ConclusionPudendal nerve block (PNB) could effectively relieve postoperative pain of hemorrhoids. However, our results still need to be confirmed by multi-center clinical studies.
OIP5-AS1/CD147/TRPM7 axis promotes gastric cancer metastasis by regulating apoptosis related PI3K-Akt signaling
Authors: Chen Jianpeng , Wang Wei , Zhang Yujie , Wang Caixia , Wang Weibo , Zheng Aiming 
Published: December 2023

BackgroundTo explore the mechanism of OIP5-AS1/CD147/TRPM7 axis to gastric cancer (GC) metastasis.MethodsBioinformatic analysis was performed to pick up the candidate genes associated with regulation GC metastasis. Using GC cell lines, AGS and MKN-45 as research objects, identify the effect of candidate genes on GC metastasis, judge cell proliferation status by MTT assay and cell clone number, and detect cell migration by Transwell and Wound-healing assay. The molecular mechanism of CD147/OIP5/TRPM7 axis regulating GC metastasis was further explored by RNA sequencing. The key signaling pathways were subsequently verified by flow cytometry and WB.ResultsBioinformatic analysis suggested OIP5-AS1/CD147/TRPM7 axis may be involving in GC metastasis. The RNA interference experiment proved that after gene interference, the proliferation ability of GC cells decreased significantly (P&lt;0.05), which was manifested in the reduction of the number of cell clones. In addition, the migration ability of GC cells was also affected, which was based on the results of Wound Healing (P&lt;0.05). CD147, OIP5-AS1 and TRPM7 all have harmful effects on GC cells. The relationship between OIP5-AS1 and CD147/TRPM7 was detected by RNA immunoprecipitation. Moreover, the RNA sequencing data indicated that CD147/OIP5-AS1/TRPM7 may coordinately regulate the PI3K-AKT pathway related to GC cell apoptosis, thereby affecting the proliferation and migration of GC cells. After RNA interference, the level of apoptosis increased both in AGS and MKN-45 cells. Meanwhile, the expression of pro-apoptotic proteins Caspase9 and BAX were up-regulated (P&lt;0.05). In addition, the expression of PI3K and AKT proteins was reduced (P&lt;0.05). The mouse tumorigenesis experiment corroborated the results of the in vitro study.ConclusionOIP5-AS1/CD147/TRPM7 axis reduces GC cell proliferation by regulating apoptosis associated with PI3K-AKT signaling, further affecting cancer metastasis.
Lesion segmentation on 18F-fluciclovine PET/CT images using deep learning
Authors: Wang Tonghe , Lei Yang , Schreibmann Eduard , Roper Justin , Liu Tian , Schuster David M. , Jani Ashesh B. , Yang Xiaofeng 
Published: December 2023

Background and purposeA novel radiotracer, 18F-fluciclovine (anti-3-18F-FACBC), has been demonstrated to be associated with significantly improved survival when it is used in PET/CT imaging to guide postprostatectomy salvage radiotherapy for prostate cancer. We aimed to investigate the feasibility of using a deep learning method to automatically detect and segment lesions on 18F-fluciclovine PET/CT images.Materials and methodsWe retrospectively identified 84 patients who are enrolled in Arm B of the Emory Molecular Prostate Imaging for Radiotherapy Enhancement (EMPIRE-1) trial. All 84 patients had prostate adenocarcinoma and underwent prostatectomy and 18F-fluciclovine PET/CT imaging with lesions identified and delineated by physicians. Three different neural networks with increasing levels of complexity (U-net, Cascaded U-net, and a cascaded detection segmentation network) were trained and tested on the 84 patients with a fivefold cross-validation strategy and a hold-out test, using manual contours as the ground truth. We also investigated using both PET and CT or using PET only as input to the neural network. Dice similarity coefficient (DSC), 95th percentile Hausdorff distance (HD95), center-of-mass distance (CMD), and volume difference (VD) were used to quantify the quality of segmentation results against ground truth contours provided by physicians.ResultsAll three deep learning methods were able to detect 144/155 lesions and 153/155 lesions successfully when PET+CT and PET only, respectively, served as input. Quantitative results demonstrated that the neural network with the best performance was able to segment lesions with an average DSC of 0.68 ± 0.15 and HD95 of 4 ± 2 mm. The center of mass of the segmented contours deviated from physician contours by approximately 2 mm on average, and the volume difference was less than 1 cc. The novel network proposed by us achieves the best performance compared to current networks. The addition of CT as input to the neural network contributed to more cases of failure (DSC = 0), and among those cases of DSC &gt; 0, it was shown to produce no statistically significant difference with the use of only PET as input for our proposed method.ConclusionQuantitative results demonstrated the feasibility of the deep learning methods in automatically segmenting lesions on 18F-fluciclovine PET/CT images. This indicates the great potential of 18F-fluciclovine PET/CT combined with deep learning for providing a second check in identifying lesions as well as saving time and effort for physicians in contouring.
Efficacy and safety of metformin for melasma treatment: a systematic review and meta-analysis
Authors: Mongkhon Pajaree , Ruengorn Chidchanok , Awiphan Ratanaporn , Phosuya Chabaphai , Ruanta Yongyuth , Thavorn Kednapa , Jamjanya Sirinda , Chuamanochan Mati , Nochaiwong Surapon 
Published: December 2023

Objective: Metformin has recently been demonstrated to have an anti-melanogenic activity. Nevertheless, clinical evidence of the effectiveness of metformin in melasma is lacking. The objective of this study was to assess the efficacy and safety of metformin in the treatment of melasma.Methods: MEDLINE, Embase, PubMed, Cochrane Library (CENTRAL), Scopus, CINAHL, and grey literature databases were searched to 4 October 2022 and updated on 26 February 2023. Randomized controlled trials (RCTs), quasi-RCTs, observational studies, case series, and case reports investigating the efficacy and safety of metformin for melasma were included. The Melasma Area Severity Index (MASI) scores that changed from baseline were pooled using fixed-effects model and expressed as standardized mean differences (SMDs) and 95% confidence intervals (CIs).Results: Three RCTs including 140 patients with melasma were included. The results demonstrated that after 8 weeks, 15% topical metformin significantly reduced the Melasma Area Severity Index (MASI) score compared to placebo (1 trial; n = 60; MD, −0.56; 95% CI, −1.07 to −0.04; p = 0.034). Furthermore, when compared to triple combination cream (TCC), 30% topical metformin demonstrated similar efficacy in reducing the MASI score after 8 weeks (2 trials; n = 80; MD, 0.19, 95% CI, −0.25 to 0.63; p = 0.390). Patients using 30% topical metformin had fewer adverse events compared to TCC users, although no statistical difference was found.Conclusion: Topical metformin was as effective as triple combination cream (TCC) in decreasing changes in the MASI score in patients with melasma, with minimum adverse events. Further studies with larger sample sizes, longer follow-up times, and well-designed trials are required.Systematic Review Registration: Identifier PROSPERO (CRD42022351966).
Female-specific pharmacotherapy in ADHD: premenstrual adjustment of psychostimulant dosage
Authors: de Jong M. , Wynchank D. S. M. R. , van Andel E. , Beekman A. T. F. , Kooij J. J. S. 
Published: December 2023

ObjectiveAttention-Deficit/Hyperactivity Disorder (ADHD) is a common neurodevelopmental condition which is underdiagnosed and undertreated in women. For decades, the ADHD field has called for more insight into female-specific therapy. Preliminary findings postulate that changes in sex hormones during the menstrual cycle may influence the effectiveness of psychostimulant medication. Yet, pharmacotherapeutic interventions tailored to women with ADHD remain scarce. Previously, our group showed an increase in mood symptoms in the premenstrual week in women with ADHD. Premenstrual worsening of depressive and ADHD symptoms represent a treatment challenge. In our adult ADHD clinic, we noted several women describing exacerbation of their ADHD and depressive symptoms in the premenstrual week and/or insufficient effect of their established dosage of psychostimulant. We responded to the need expressed by these women by increasing their stimulant dosage in the premenstrual week, while monitoring the response and side effects.MethodsThis community case study of nine consecutive women being treated for ADHD and co-occurring conditions (including depression and premenstrual dysphoric disorder), reports our local experience of increasing the individually prescribed psychostimulant dosage during the premenstrual period. We methodically monitored the effect of this increased dosage on ADHD symptoms, mood and somatic symptoms for the following 6–24 months.ResultsWith premenstrual dose elevation, all nine women experienced improved ADHD and mood symptoms with minimal adverse events. Premenstrual inattention, irritability and energy levels improved, and now resembled the other non-premenstrual weeks more closely. All women decided to continue with the elevated premenstrual pharmacotherapy.DiscussionOur preliminary results demonstrate potential benefits of increasing premenstrual psychostimulant dosage in women with ADHD, experiencing premenstrual worsening of ADHD and mood symptoms. The results concur with previous findings of diminished response to amphetamines in the late luteal phase. Increased dosage may help combat premenstrual worsening of cognitive and emotional symptoms in women with ADHD, with significant clinical implications. Better management of premenstrual ADHD and mood symptoms in vulnerable women can improve treatment outcome and meet an unmet need. However, implementation should be individually explored. Further investigation of luteal phase psychostimulant dose adjustment is required for safe, optimal and individualised treatment for women with ADHD.