bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of pregnancy vitamin D supplementation on maternal blood pressure: real-world data analysis within the MAVIDOS randomised placebo-controlled trial
Authors: Citeroni-Clark Natasha L. , D’Angelo Stefania , Crozier Sarah R. , Kermack Alexandra , Godfrey Keith M. , Cooper Cyrus , Harvey Nicholas C. , Moon Rebecca J. 
Published: January 2025

Abstract

Purpose
Observational studies have suggested negative associations between maternal 25-hydroxyvitamin D (25(OH)D) status and risk of hypertensive disorders of pregnancy [pregnancy-induced hypertension (PIH) and preeclampsia (PET)]. Data from intervention studies are limited. We hypothesised that vitamin D supplementation would lower maternal blood pressure (BP) during pregnancy and reduce the incidence of hypertensive disorders of pregnancy.

Methods
The Maternal Vitamin D Osteoporosis Study (MAVIDOS) was a randomised placebo-controlled trial. Pregnant women with a baseline 25(OH)D of 25–100 nmol/l were randomized to either 1000 IU/day cholecalciferol or placebo from 14 to 17 weeks’ gestation until delivery. BP recordings documented during routine clinical pregnancy care were obtained from clinical records and grouped into gestational windows based on the schedule for routine antenatal care in the United Kingdom (23+0–24+6, 27+0–28+6, 33+0–35+6, 37+0–38+6, 39+0–40+6 and ≥ 41+0 weeks+days). Systolic and diastolic BP measurements in these gestational windows were compared between randomisation groups. Diagnoses of PIH or PET (in accordance with national guidelines) and the use of antihypertensive agents were also noted and compared between groups.

Results
Data for 734 women (366 cholecalciferol, 368 placebo) were included. Maternal mean systolic and diastolic BP did not differ between the randomization groups at any of the gestations studied. The incidences of PIH (placebo 1.6%, cholecalciferol 3.6%, p = 0.10) and PET (placebo 3.3%, cholecalciferol 3.8%, p = 0.68) were similar between the two groups.

Conclusions
Gestational vitamin D supplementation with 1000 IU/day from 14 to 17 weeks gestation did not lower maternal BP or reduce the incidences of PIH or PET in this trial.
Higher bone mineral density at six years of age in very preterm-born infants fed human milk compared to formula feeding. A secondary analysis of an RCT
Authors: Toftlund Line H. , Beck-Nielsen Signe Sparre , Agertoft Lone , Halken Susanne , Zachariassen Gitte 
Published: January 2025

Abstract
In very preterm-born infants, nutritional intake is important to reduce the risk of severe metabolic bone disease including the risk of a lower bone mineral density (BMD). The aim of this study was to evaluate bone mineral content (BMC) and BMD (measured as BMC per bone area (BA)) at six years of age in very preterm-born infants fed different diets post-discharge. Data on this topic so far is insufficient, and with this study we aim to supply more useful data. A prospective follow-up study of 281 children born very preterm (gestational age ≤ 32 + 0 weeks) and enrolled in a multicentre RCT on post-discharge nutrition. Infants fed human milk (HM) were randomised respectively to be fed unfortified HM (UHM) or fortified human milk (FHM) from hospital discharge to four months’ corrected age. Those not fed HM received a preterm formula (PF). At six years of age, BMD and BMC in all the children were established by means of a dual-energy X-ray absorptiometry (DXA) scan (Lunar Prodigy) and adjusted for sex, age, and anthropometrics. A total of 192 very preterm-born children (59 fed UHM, 67 FHM and 66 PF) had a DXA scan performed at median 6 (5.8—8.3) years of age. No significant difference was found comparing UHM and FHM according to height, weight, BA, BMC, and BMD at six years of age. However, a multiple regression analysis showed significantly improved BMD in breastfed children compared to PF-fed children.

Conclusions: Fortified compared to non-fortified human milk post-discharge did not have an impact on BMD at 6 years of age in very preterm-born infants. Breastfed children demonstrated higher BMD than formula-fed children.

What is Known:

• Adequate nutritional intake is important to improve growth and to reduce the risk of severe bone disease in very preterm born infants.

• Bone mineralization is attained later in preterm born infants compared to term born infants.

What is New:

• Feeding human milk with fortification compared to non-fortified human milk did not improve bone mineral density in children born very preterm in this follow-up study at six years of age.

• Feeding human milk compared to formula was associated with increased BMD at six years of age among very preterm born infants.
Epigenetic memory as crucial contributing factor in directing the differentiation of human iPSC into pancreatic β-cells in vitro
Authors: Diane Abdoulaye , Mu-U-Min Razik Bin Abdul , Al-Siddiqi Heba Hussain 
Published: January 2025

Abstract
Impaired insulin secretion contributes to the pathogenesis of type 1 diabetes mellitus through autoimmune destruction of pancreatic β-cells and the pathogenesis of severe forms of type 2 diabetes mellitus through β-cell dedifferentiation and other mechanisms. Replenishment of malfunctioning β-cells via islet transplantation has the potential to induce long-term glycemic control in the body. However, this treatment option cannot widely be implemented in clinical due to healthy islet donor shortage. Emerging β-cell replacement with human-induced pluripotent stem cell (iPSC) provides high remedial therapy hopes. Thus, tremendous progress has been made in developing β-cell differentiation protocols in vitro; however, most of the differentiated iPSC-derived β-cells showed immature phenotypes associated with low efficiency depending on the iPSC lines used, creating a crucial barrier for their clinical implementation. Multiple mechanisms including differences in genetic, cell cycle patterns, and mitochondrial dysfunction underlie the defective differentiation propensity of iPSC into insulin-producing β-cells. Accumulating evidence recently indicated that, following the reprogramming, epigenetic memory inherited from parental cells substantially affects the differentiation capacity of many iPSC lines. Therefore, differences in epigenetic signature are likely to be essential contributing factors influencing the propensity of iPSC differentiation. In this review, we will document the impact of the epigenome on the reprogramming efficacy and differentiation potential of iPSCs and how targeting the epigenetic residual memory could be an additional strategy to improve the differentiation efficiency of existing protocols to generate fully functional hPSC-derived pancreatic β-cells for diabetes therapy and drug screening.
How effective and sustainable is proctoring in robotic surgery? A retrospective analysis based on interviews with surgeons
Authors: Günther Veronika , Nees Frauke , Maass Nicolai , von Otte Sören , Ruchay Zino , Pape Julian , Ackermann Johannes , Alkatout Ibrahim 
Published: January 2025

Abstract

Background
Robot-assisted surgery is one of several minimally invasive techniques which have become increasingly important in recent years. Education and training are key factors of sustainable success, and surgical tutoring by an experienced external surgeon (proctoring) has emerged as a very useful method of training surgeons. Proctoring enables surgeons to train their respective skills and eventually improve the overall quality of surgical care.

Methods
To evaluate the sustainability of proctoring, we conducted telephone interviews with colleagues who participated in a single-center surgical proctoring program. The aim was to analyze the feasibility of the concept for being established in the future, how well it suits the surgeons (depending on their individual work experience), and which areas of clinical practice would benefit from proctoring. Furthermore, the surgeons provided free-text comments to the questions on a feedback form handed out after the proctoring.

Results
Surgical skills were significantly enhanced and operating times were reduced after proctoring. Given favorable structural conditions, the number of cases and the case-mix index were also significantly increased. It was found that the learning needs of surgeons differ, depending on their medical function, work experience, and the frequency of robotic surgery. On the feedback forms, proctoring was consistently rated as a positive and supportive measure.

Conclusions
Proctoring is an important aspect of the individual development of surgeons. Depending on learning needs, a structured curriculum is crucial and should be established for the future. Regular feedback modules should be a part of any surgical training concept.
Evaluation of ILAR and PRINTO classifications for juvenile idiopathic arthritis: oligoarticular JIA vs early-onset ANA-positive JIA
Authors: Küçükali Batuhan , Yıldız Çisem , Gülle Buğra Taygun , Gezgin Yıldırım Deniz , Bakkaloğlu Sevcan A. 
Published: January 2025

Abstract

Objectives
The International League of Associations for Rheumatology (ILAR) juvenile idiopathic arthritis (JIA) classification was revisited by the Pediatric Rheumatology International Trials Organization (PRINTO) in 2018. Classifications should establish uniform groups to assist physicians in providing optimal care. Therefore, we evaluated changes proposed by PRINTO to highlight their impact on forming consistent groups regarding uveitis and treatment responses, particularly focusing on early-onset anti-nuclear antibody (ANA)-positive JIA.

Methods
Pediatric patients diagnosed with JIA according to ILAR and PRINTO classification, with a minimum of 1-year of follow-up, were enrolled, excluding those meeting the exclusion criteria for both the oligoarticular JIA and the early-onset ANA-positive JIA groups.

Results
Among the 139 enrolled patients, 110 (79.1%) had oligoarticular JIA, while 15 (10.8%) had early-onset ANA-positive JIA. The below-age-5 criterion demonstrated the strongest association with uveitis, while the below-age-7 provided similar associations without substantial exclusions (odds ratio (OR) 8.62 [2.50–29.81] vs 7.45 [2.37–26.66]). Patients with a single ANA positivity at a titer ≥ 1/160 and age of onset below 7 had a notably higher risk of new-onset uveitis and biologic DMARD requirement (OR 7.95 [2.37–26.66] and 3.6 [1.42–9.09], respectively).

Conclusion
The inclusion of age of disease onset and ANA positivity with a titer ≥ 1/160 has enhanced uniformity in uveitis risk and treatment response, including failure of conventional synthetic DMARDs. Additionally, a single ANA positivity at a ≥ 1/160 titer rather than requiring two instances yields similar consistency. However, the joint count criteria failed to form consistent groups. PRINTO’s classification places a significant proportion of patients into the “other JIA” group, necessitating further classification for improved clinical utility.

Key Points•Inclusion of age and ANA positivity criteria increased uniformity among the subgroups.•Single ANA positivity at a ≥ 1/160 titer can be sufficient instead of twice.•Early utilization of bDMARDs may be beneficial for early-onset ANA-positive JIA group.•PRINTO classification must further classify the “other JIA” before being implemented in clinical practice.
Radiographic entheseal lesions of the pelvic region are more prevalent in radiographic axSpA than in age- and sex-matched controls and are associated with more severe spinal disease
Authors: Wink F. R. , Diemel T. , Arends S. , Spoorenberg A. 
Published: January 2025

Abstract

Objectives
In axial spondyloarthritis (axSpA), entheseal involvement is common, which contributes significantly to disease burden and may also lead to structural damage. Although radiographs of the pelvis are widely available in axSpA, information on entheseal damage and associated characteristics are lacking. Therefore, we assessed the prevalence of radiographic entheseal lesions at the pelvic region in radiographic (r-) axSpA compared with controls and explored associations with patient and disease characteristics.

Methods
Pelvic radiographs of 167 consecutive r-axSpA patients were randomized with 100 pelvic radiographs from age- and sex-matched controls. Radiographs were blinded for patient information and sacroiliac joints and bilaterally evaluated for erosions/cortical irregularities, enthesophytes, and calcifications by two trained readers at the greater and lesser trochanter, os ischium, and iliac crest.

Results
Entheseal lesions were observed in 127 (76%) of r-axSpA patients and 58 (58%) controls. R-axSpA patients showed significantly more (bilateral) entheseal lesions than controls at all entheseal sites. Most lesions were found at the os ischium, erosions/cortical irregularities were most prevalent, and calcification was the most specific lesion in r-axSpA. Patients with lesions were significantly older, had longer symptom duration, and more severe spinal radiographic damage than patients without lesions. Enthesophytes were found significantly more often in patients with body mass index (BMI) ≥ 25.

Conclusion
Structural entheseal lesions observed at pelvic radiographs are not specific but occur often in r-axSpA patients. Treating physicians should keep in mind that these entheseal lesions are associated with more severe axial disease and high BMI which may be relevant for treatment decisions.

Key Points• Pelvic radiographic entheseal lesions are significantly more prevalent in r-axSpA patients than in controls.• Radiographic entheseal lesions are associated with longer symptom duration and more spinal radiographic damage.• Pelvic enthesophytes are found significantly more often in r-axSpA patients with BMI ≥ 25.
An advanced regional integrated water vapor estimation model utilizing least squares support vector machine for the upper Rhine graben region
Authors: Wang Lingke , Kutterer Hansjörg 
Published: January 2025

Abstract
Integrated Water Vapor (IWV) is crucial in environmental research, offering insights into atmospheric dynamics. Direct IWV measurement is challenging, necessitating alternative estimation technologies. Existing methods including Global Navigation Satellite System (GNSS), radiosondes, water vapor radiometers (WVR), satellite remote sensing, and numerical weather models (NWM), have specific limitations. GNSS and WVR provide high precision and temporal resolution (e.g., 5 min) but are limited to specific locations. Radiosondes, while accurate, have sparse spatial distribution and low temporal resolution (e.g., twice daily). Satellite remote sensing offers broad spatial resolution but lower temporal resolution (hours to days) and reduced accuracy under cloudy conditions and due to satellite tracks. NWMs provide global hourly products but their accuracy depends on meteorological data and model precision.
This study introduces a regional IWV predictive model using Machine Learning to address these challenges. Utilizing IWV data from GNSS stations, the study develops a predictive model based on least squares support vector machine, which autonomously determines optimal parameters to enhance performance. The model enables accurate IWV estimation at any location within a region, using inputs such as latitude, longitude, altitude, and temperature, achieving an average root mean square error of 0.95 mm. The model’s performance varies across seasons and terrains, showing adaptability to diverse conditions. The model’s reliability is validated by comparing its predictions with the conventional ERA5 IWV method, showing a 61% improvement rate. This refined IWV estimation model is applied for regional climate analysis, demonstrating its practical utility in environmental research, specifically for the Upper Rhine Graben Region.
Does the use of engorged adult ticks of Rhipicephalus microplus as substrate modifies the acaricidal behavior of Metarhizium anisopliae?
Authors: Fernández-Salas Agustín , Romero-Pérez Juan Bernardo , Alonso-Díaz Miguel Ángel 
Published: January 2025

Abstract
Entomopathogenic fungi (EPF) is one of the most promising alternatives to regulate tick populations. However, these fungi may lose acaricidal effectiveness over time, due to the storage period and/or successive cultivation on artificial media. It is known that using arthropod pests as a substrate for EPF could potentially alter their acaricidal behavior over time, however, studies using ticks for this purpose are scarce. Hence, the aims of this research were: (1) to isolate strains of Metarhizium anisopliae from paddocks of cattle farms, (2) to evaluate the effect of engorged adult ticks of Rhipicephalus microplus as a substrate on the tickicide behavior of Metarhizium anisopliae strains, and (3) to determine the lethal time of each M. anisopliae strain to kill 50% (LT50) and 99% (LT99) of engorged ticks. First, the natural acaricidal effect of 10 strains of M. anisopliae sensu lato isolated from soils of cattle farms on R. microplus was evaluated. Second, the influence of substrate (R. microplus, Galleria mellonella and Sabouraud dextrose agar [SDA]) on the acaricidal activity and virulence index (lethal time) of each strain during four generations was evaluated. Strains MaV69, MaV60 and MaV67 showed more than 90% mortality at day 20 post-treatment. The use of engorged adult ticks as substrate increased the virulence of five M. anisopliae s.l. strains. Larvae of G. mellonella and SDA as substrate did not modify the acaricidal behavior of the 10 strains evaluated. Seven of ten EPF decreased their LT50 and three decreased their LT99 as an index of their virulence enhancement.
Exploring macroinvertebrate community assembly rules: unraveling the effects of flow intermittency and poor ecological potential on environmental filtering and limiting similarity through functional traits
Authors: Szeles Júlia , B-Béres Viktória , Bozóki Tamás , Fekete Judit , Ficsór Márk , Boda Pál , Várbíró Gábor 
Published: January 2025

Abstract
Flow intermittency and poor environmental conditions can occur separately or simultaneously, affecting the occurrence of trait states in stream macroinvertebrate assemblages. Within the framework of limiting similarity theory, we investigate whether environmental filtering or limiting similarity serves as the primary driver force under three environmental conditions: unstressed (perennial environment and good ecological potential), single-stressed (intermittent condition or poor ecological potential), and multi-stressed (intermittent condition and poor ecological potential) environments. Our analysis validated neutral trait states that are not influenced by different environmental stressors. In unstressed environments, limiting similarity is the primary driver force shaping the macroinvertebrate community. In stressed environments, however, the type of stress determines the influence of environmental filtering. It is not the quantity but rather the quality of stress that increases the influence of environmental filtering. Flow intermittency has a more pronounced effect on the aquatic community than poor ecological potential because it eliminates living conditions, while poor ecological conditions merely restrict them. As anticipated, we observed that certain trait states have specific responses indicating that different driver forces influence them across various environmental conditions. Our study provides valuable insights for managing freshwater habitats and developing adaptive conservation strategies considering increasing drought frequency worldwide.
Hybrid Nanofluids-Based Direct Absorption Solar Collector: An Experimental Approach
Authors: Huminic Gabriela , Huminic Angel , Fleacă Claudiu , Dumitrache Florian 
Published: January 2025

Abstract
In the last years, studies have demonstrated the potential of hybrid nanofluids to enhance the performance of direct absorption solar collectors. These working fluids containing noble metals (gold, silver) are known for their local surface plasmon resonance which is the main cause for the increased absorption within the solar spectrum. In the current paper, new direct absorption solar collectors prototypes using water-ethylene glycol solution and 0.1 wt.% silver nanoparticles + reduced graphene oxide dispersed in water-ethylene glycol mixture were designed, built, and tested under outdoor conditions, at two flow rates (1.0 and 1.5 l·min−1) and two inlet temperatures (20

$$^\circ{\rm C}$$

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C

and 30

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C

), over several days in September 2023, at Brasov, Romania. The results suggested that by using silver nanoparticles + reduced graphene oxide nanofluid, the efficiency is improved related to water-ethylene glycol solution. The maximum relative enhancement in efficiency was 16.72% related to the base fluid. Also, at 1.0 l·min−1, the instantaneous and accumulative energies delivered were about 13.51% and 42.91%, respectively, higher than the water-ethylene glycol solution. Finally, the current results were compared to other research carried out on full-scale direct absorption solar collectors tested in outdoor conditions.
Operationalizing the ICF Core Sets for Autism and ADHD: A Multiple-Methods Feasibility Study
Authors: Alehagen Lovisa , Hasslinger John , Wessman Elina , Black Melissa , Lundin Remnélius Karl , Helander Johan , Zander Eric , Bölte Sven 
Published: January 2025

Abstract
The International Classification of Functioning, Disability and Health (ICF) is the World Health Organization’s (WHO) standard for assessing individual functioning. Over the last decade, the ICF has been made more accessible for autism and ADHD through the development and validation of tailored shorter ICF versions for these diagnoses, ICF Core Sets. To further enhance their applicability in research and practice, these Core Sets have been operationalized and implemented on an online platform, the ICF CoreSets platform. Here, we describe the iterative development process of the CoreSets platform. This multiple-methods study examines user feedback on the operationalization of the Core Sets and the feasibility of the CoreSets platform as a functional assessment for autism and ADHD. We collected a total of 678 assessments from the CoreSets platform. Individuals diagnosed with autism and/or ADHD, their relatives, participants from the general population, and professionals completed and provided feedback on the usability of the CoreSets platform. Qualitative feedback via interviews and focus groups were also collected. Qualitative data were analysed via content analysis, while quantitative data were examined using univariate and descriptive techniques. Findings show that the ICF CoreSets platform is feasible and user-friendly, but areas for improvement were also indicated, leading to additional refinement of the operationalization and platform. The operationalization of the ICF Core Sets and their implementation in the CoreSets platform appears adequate for use in research and practice, particularly after revisions indicated by future users, and is now ready for psychometric standardization.