bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional characterization of hemocyte homeostasis associated protein, HHAP in two species of crustaceans
Authors:   , Apitanyasai Kantamas 
Published: November 2023

Hemocyte homeostasis-associated protein (PmHHAP) has been reported as a viral responsive protein that plays an important role in controlling hemocyte homeostasis in shrimp Penaeus monodon. In this study, the role of PmHHAP in regulating apoptosis in shrimp was investigated. In vivo gene silencing of PmHHAP could induce the high level of apoptosis in shrimp hemocytes and altered the expression of apoptosis-related genes particularly PmCasp and PmCaspase. Moreover, PmHHAP was able to bind to PmCasp and the rPmHHAP protein could decrease apoptosis in the rPmCasp-treated hemocytes cells. These results indicated that PmHHAP is an anti-apoptosis protein that regulates hemocyte homeostasis by inhibiting apoptosis in P.monodon. Yeast two-hybrid and co-immunoprecipitation revealed that PmHHAP binds to an anti-apoptosis protein of white spot syndrome virus, WSSV134. The viral protein WSSV134 is a late protein of WSSV which is initially expressed after 24-hour post infection (hpi) and is likely involved in viral propagation. Co-silencing of PmHHAP and WSSV134 prior to WSSV infection showed significant increase of caspase activity, which was higher than silencing only PmHHAP or WSSV134, suggesting that these two proteins might work concordantly to control apoptosis during WSSV infection. Suppression subtractive hybridization (SSH) of PmHHAP silenced shrimp hemocytes identified several viral responsive genes as well as hemocyte homeostasis-related genes such as the transcription factor ATF-β, cathepsin L, lactate dehydrogenase, ferritin and inhibitor of apoptosis (IAP), some of which should be selected for further investigation. In addition, a homolog of HHAP in crayfish Pacifastacus leniusculus with 47% similarity to PmHHAP was functional characterized. PlHHAP was detected in all examined tissues but highly expressed in intestine and gill. Surprisingly, PlHHAP was found to play important role in bacterial but not viral defense in contrast to PmHHAP. PlHHAP transcript remained unchanged upon WSSV infection but dramatically increased upon bacterial Aeromonas hydrophila B1 challenge. Furthermore, suppression of PlHHAP resulted in increasing the number of bacteria in intestine but did not affected hemocyte apoptosis or the expression of genes (CHF, astakine1, astakine2, and IGFBP7) involved in crayfish hematopoiesis and hemocyte homeostasis suggested that HHAP from different crustacean species have different functions in defense against invading pathogens.
Molecular characterization of isoamylases from cassava tuber Manihot esculenta Crantz ‘KU50’
Authors:   , Panpetch Pawinee 
Published: November 2023

Starch debranching enzyme or isoamylase (EC.3.2.1.68), an important enzyme in starch metabolism, catalyses the hydrolysis of α-1,6 glycosidic linkages of amylopectin. In this work, cassava isoamylase genes were isolated from cDNA generated from total RNA from tubers of Manihot esculanta Crantz cultivar KU50 by RT-PCR and analysed by agarose gel electrophoresis. Three putative isoamylase genes, MeISA1, MeISA2 and MeISA3 were identified and amplified excluding the fragments encoding their transit peptides. Full length of MeISA3 gene was obtained by 5’ Rapid Amplification of cDNA Ends (5’ RACE). The mature MeISA1 and MeISA2 were successfully amplified and cloned into a pETDuet1 vector, while the mature MeISA3 was amplified and cloned into a pET21b and sequenced. The ORFs of mature recombinant MeISA1, MeISA2 and MeISA3 genes were 2,292, 2,649 and 2,127 kb, encoding 763, 882 and 708 amino acid with substantial similarity to StISA1, StISA2 and StISA3 from potato at 84.4%, 68.9% and 80.9%, respectively. Then, rMeISA1 and rMeISA2 were coexpressed and rMeISA3 was single expressed in Escherichia coli SoluBL21(DE3) and purified by HistrapTM column. Analysis by SDS-PAGE and immunoblot showed approximate molecular weights of 87, 99, and 80 kDa, respectively. Debranching activity was only detectable in the fractions where both rMeISA1 and rMeISA2 were present. The heteromultimeric DBE from 4-5 h induced culture analysed by gel filtration chromatography and Western blot showed combinations of rMeISA1 and rMeISA2 at ratios of 1:1 to 1:4. Pooled fractions of rMeISA1/rMeISA2 with DBE activity and the purified rMeISA3 were used for enzyme characterisation. Both rMeISA1/rMeISA2 and rMeISA3 showed the optimum activity at 37ºC but their optimum pH were at 7.0 and 6.0, respectively. Enzyme activity of rMeISA1/rMeISA2 was enhanced by Co2+, Mg2+ and Ca2+, whereas rMeISA3 activity could be significantly activated by Mn2+ and Co2+. Cu2+ was a strong inhibitor for both enzymes. Debranched amylopectin products analysed by HPACE-PAD showed chain length distributions typical of plant debranching enzymes with DP from 6 to 20 with major products around DP 10 to 12. rMeISA1/rMeISA2 were specific for amylopectin while rMeISA3 showed the highest activity on beta-limit dextrin. However, they could hardly hydrolyse pullulan at all, indicating their classification as isoamylase-type debranching enzyme. The debranching activity of rMeISA1 and rMeISA2 required their association as heteromeric complexes.
Free radical scavenging and antiproliferative activities of peptide from hygroscopic earthstar astraeus hygrometricus
Authors:   , Tunyatarinun Natpatsorn 
Published: November 2023

Mushrooms have been used as food and for therapeutic purpose for decades. Indeed, various compounds derived from them have potential biological activities. The present study aims to explore the free radical scavenging properties of peptides derived from the edible mushroom Astraeus hygrometricus. In this work, peptides from A. hygrometricus protein hydrolysates attainable with microbial proteases (Alcalase®, Flavourzyme® and Neutrase®) were prepared and their antioxidant activities were determined. Peptide fractions derived from A. hygrometricus hydrolyzed by 1% Alcalase®, showed the highest DPPH and ABTS radical scavenging activities with IC50 values of 13.40±0.48 and 5.53±0.59 µg/mL, respectively. Peptide fractions were obtained using molecular weight cut-offs of 10, 5, 3 and 0.65 kDa membranes and their antioxidant properties were further analyzed. Among the fractions, MW < 0.65 kDa (F5 fraction) exhibited high levels of free radical scavenging activities towards DPPH and ABTS with IC50 values of 4.84±0.26 and 1.49±0.39 µg/mL, respectively. The F51 fraction from the Superdex® 75 column was identified as having the highest scavenging activities on DPPH and ABTS radicals with IC50 values of 32.47±1.16 and 15.58±0.73 µg/mL, respectively. Furthermore, the F51 fraction could protect hydroxyl radical-induced DNA damage as shown in pKS, pUC19, and pBR322. In addition, fraction F51 was used to determine antiproliferative activity. The results showed that purified peptide fraction F51 was not cytotoxic on normal and cancer cell lines, and the lung cancer cell line (Chago-k1 cell) had the highest antiproliferative activity. Moreover, fraction F51 was used for determining the induction of apoptosis that used flow cytometry and the caspase 3, 8 and 9 activities analysis method. The results indicate that purified peptide fraction F51 could induce the highest apoptotic cells and caspase 3, 8 and 9 activities on Chago-k1 cell at 72 hours. Furthermore, the peptide fraction F51 was purified using RP-HPLC and sub-fractions F51-1 to F51-5 collected. The result was found to possess the highest antioxidant activity on fraction F51-5. Finally, the amino acid sequences of sub-fractions F51-1 to F51-5 were identified using MS/MS (ESI-Q-TOF). The amino acid sequences of the purified peptide fractions F51-1 to F51-5 in respective order were as follows: Val-Thr-Thr-His-Lys-Thr-Val-Thr-Lys-His (VTTHKTVTKH, 1152 Da), Met-Pro-His-His-Tyr-Thr-Ile-Phe-Ala-Leu-Gly-Thr-Gln-Ser-Arg-Pro-Ser (MPHHYTIFALGTQSRPS, 1942 Da), Pro-Ser-Arg-Gly-Ser-Glu-Arg-Ala-Arg-Ala (PSRGSERARA, 1086 Da), Thr-Leu-Ser-Asn-Leu-Gly-Leu-Val-Leu-Val-His (TLSNLGLVLVH, 1165 Da) and Leu-Thr-Asn-Ser-Pro-Trp-Ala-His-Ala (LTNSPWAHA, 996 Da). Hence, the purified peptide fraction F51 could be used as a natural antioxidant for drug development.
”Oli kyllä ajatuksena, että suorittais sitten sitä töiden ohella loppuun”
Authors: Nori Hanna , Isopahkala-Bouret Ulpukka , Vanttaja Markku 
Published: November 2023

Artikkelissa tarkastelemme yliopistossa vähintään kymmenen vuotta kirjoilla olleita eli "ikuisia opiskelijoita". Kysymme, miten taustaan ja elämäntilanteeseen liittyvät tekijät ovat yhteydessä opintojen pitkittymiseen. Tutkimuksessa on hyödynnetty rekisteripohjaista ja haastatteluihin pohjautuvia aineistoja. Teoreettisena viitekehyksenä käytämme Vincent Tinton integraatioteoriaa. Opintojen pitkittymisen riskitekijöitä olivat opintojen varhainen aloitusikä, vanhempien pienituloisuus, generalistialalla opiskelu, perheellisyys ja hyväpalkkaiseen työhön päätyminen jo opiskeluaikana. Aineistoista löysimme neljä ikuisten opiskelijoiden profiilia: jumiutuneet, pitkittynyttä nuoruutta viettävät, perheelliset ja urakeskeiset. Kaikilla opiskelun haasteita määritti rakenteellisten seikkojen ohella myös opiskelijoiden aikaisempi elämänhistoria ja elämäntilanteet.
Korrekturhinweis zu WUG 45 (4)
Authors: Theurl Simon , Tamesberger Dennis 
Published: November 2023

In der Berechnung der Kosten einer Jobgarantie für Langzeitbeschäftigungslose in Österreich ist ein Fehler unterlaufen wodurch sich die zusätzlichen Kosten für einen Arbeitsplatz pro langzeitbeschäftigungsloser Person/pro Jahr auf 8228,8 € erhöhen (ursprünglich wurde mit Kosten in der Höhe von 6.7858 €gerechnet). Die Neuberechnung wurde mithilfe des Brutto-Netto Rechners des Finanzministeriums 2020 durchgeführt (das ist die zum Zeitpunkt der Korrektur früheste Verfügbare Version). Dadurch kam es zu Veränderungen der Dienstgeberabgaben in den Ausgaben und Rückflüssen (8.091,6 € anstelle von 8.164,4 €). Die Veränderung der Kosten, der Fehler in der ursprünglichen Berechnung, ist auf eine Überschätzung der SV-Beiträge der ArbeitgeberInnen zurückzuführen. Dieser wurde für die Neuberechnung korrigiert und anstelle von 7.643 € wurde der Betrag auf 6.126 (inkl. BMVK) € angepasst. In Summe würde die Finanzierung von Vollzeitarbeitsplätzen für 40.000 Langzeitbeschäftigungslose das Budgetdefizit im ersten Jahr nach den korrigierten Berechnungen um 329 Mio. Euro erhöhen (anstelle von 271,4 Mio. Euro in der ursprünglichen Berechnung). Für den Fehler und etwaige Unannehmlichkeiten möchten sich die Autoren hiermit aufrichtig entschuldigen.

Der ursprüngliche Artikel im Archiv: Dennis Tamesberger, Simon Theurl, (2019), Vorschlag für eine Jobgarantie für Langzeitarbeitslose in Österreich, Wirtschaft und Gesellschaft 2019, Band 45 Nr.4, S471-49 PDF Herunterladen
Klassismus
Authors: Hofmann Julia , Walter Julia 
Published: November 2023

Aumair, Betina/Theißl, Brigitte (2020). Klassenreise. Wie die soziale Herkunft unser Leben prägt. Wien, ÖGB Verlag. ISBN: 978-3-99046-429-8
Altieri, Riccardo/Hüttner, Bernd (Hg.) (2020). Klassismus und Wissenschaft. Erfahrungsberichte und Bewältigungsstrategien. Marburg, BdWi-Verlag. ISBN: 978-3-939864-28-8
Seeck, Francis/Theißl, Brigitte/Aktivist*innen der Solidarischen Aktion Neukölln, Aktivist*innen der Solidarischen Aktion (2020). Solidarisch gegen Klassismus. Organisieren, intervenieren, umverteilen. Unrast Verlag. ISBN: 978-3-89771-296-6
Poland: Policies dealing with the inflation crisis
Authors: Grodzicki Maciej J. , Surmacz Tomasz , Mozdzen Michal 
Published: November 2023

The aim of the national case study is to investigate the recent inflationary developments in Poland, against the background of tendencies present in the EMU economies. It provides insights into the structural aspects of inflation in Poland, as well as into its outcomes for growth prospects and income distribution. For this purpose, the main tendencies in nominal wages and corporate mark-ups, as well as the up-to-date strategies of the government and domestic trade unions are analysed.

The study combines quantitative analysis with qualitative overview of policy making and the activities of social partners, including the trade unions. First, we demonstrate that post-pandemic inflationary dynamics in Poland are similar to the CEE average, yet much higher than in the EMU. This can be attributed to the composition of the consumption basket, with a relatively high share of energy, food and transport expenses. There are also inflationary pressures from a strong expansion, as reflected in low unemployment, full capacity utilisation and exchange rate depreciation.
Second, we identified strong redistributive effects of the inflation in Poland, as reflected in declining real wages and labour share in national income. We see extraordinarily high mark-ups and profits in the business sector, particularly in 2021. Finally, we identify the social dialogue in Poland as conflictual, with virtually no coordinated efforts to limit the rate of inflation. Under such conditions, government policies are of even higher importance. These focused primarily on mitigating the social costs of inflation for households, and mostly took form of economy-wide tax cuts and transfers.
Fine particle liberation in saturated porous media under non-isothermalfluid flow
Authors: Zhai Xinle , Atefi-Monfared Kamelia 
Published: November 2023

Decline in well productivity is a widely reported phenomenon and a critical challenge negatively impacting energy and water operations in deep geological reservoirs [1, 2]. A key contributor to this problem is the detachment of in-situ fine particles present in the porous matrix, which will then migrate and travel through the porous formation until getting strained within thin pore throats resulting in pore clogging and therefore permeability damage [3]. The detachment of in-situ fine particle occurs when the mechanical equilibrium of the attaching (i.e., electrostatic and gravity forces) and the detaching forces (i.e., drag and lifting forces) exerted on the particle is disturbed. In geological reservoirs, the equilibrium of in-situ fines can be disturbed as a result of fluid flow velocities, temperature alterations in the porous formation, or reduced ionic strength of the in-situ fluids [4]. Fines migration and straining can also alter in-situ stresses through generating pore pressure changes as a result of permeability damage [5].
Multiple experimental and numerical studies have evaluated the mechanisms involved in detachment, migration, and straining of in-situ fines and the clogging of pore fluid channels [6, 7]. A number of studies have also focused on evaluating temperature-induced particle mobilization [8]. Variations of the electrostatic force with temperature is often explained through the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory [9]. In a saturated porous formation containing in-situ fines, Dielectric permittivity of pore fluid decreases with an increase in temperature, weakening the repulsion between the fine clay particles and the sand surface [10]. As a result, the attaching electrostatic forces are lower under higher temperatures.
This study focuses on developing a theoretical model to evaluate the impact of the size distribution of in-situ fine particles on non-isothermal fluid flow induced fine mobilization in saturated porous media. Expressions for drag force and electrostatic force are obtained based on the DLVO theory considering coupled effects of fluid velocity, temperature, and ionic strength of in-situ fluids. The main parameters adopted in the proposed model are presented in Table 1. The proposed model predicts the maximum concentration of retained fines considering coupled effects from temperatures and pore pressures. Results are valuable for estimating permeability damage and well productivity during enhanced geothermal operations.
Ovarian non-Hodgkin lymphoma with central nervous system involvement: A case report
Authors: Nyamu Brenda , Cheserem Eunice 
Published: November 2023

Background: Ovarian lymphoma is rare. Extranodal non-Hodgkin lymphoma (NHL) represents 0.5% of malignant genital diseases. Due to its rarity, there are no accepted treatment protocols. Diffuse large B cell lymphoma (DLBCL) has a central nervous system (CNS) involvement rate of 3-5%.
Case presentation: A 38-year-old presented to the gynecological oncology clinic with abdominal pain and distension. Her tumor marker CA-125 levels were elevated. She developed right-sided facial nerve palsy and blindness. A diagnosis of aggressive NHL with a differential of DLBCL was made by histopathological and immunohistochemical examination of the excised ovarian tissue. A head computed tomography scan revealed subtle enhancing opacity suspicious of lymphomatous deposits. She was lost to follow-up after ten months of chemotherapy and cranial radiotherapy.
Conclusion: Primary lymphoma of the ovary is rare, and the prognosis is often poorer when compounded with CNS involvement. Because of poor prognosis in the presence of CNS involvement, this case highlights the need for timely diagnosis and management.
Schizophrenia Of the Main Character In Fear Of Rain Movie Directed By Castille Landon
Authors: Nijam Infrida 
Published: November 2023

Literature is something that cannot be separated from people's lives, and films are one of the most popular forms of literary works today. Mental health is an important focus in everyone's life, to maintain and maintain sanity for a better life. This study discusses mental health problems, namely schizophrenia, with films as research objects. The purpose of this study was to find out the early symptoms of schizophrenia, the impact of schizophrenia, and the role of parents in responding to children with schizophrenia. This research uses descriptive qualitative method where the data collected is in the form of words, pictures and not numbers. Schizophrenia has two types of initial symptoms, namely positive symptoms and negative symptoms. In "FEAR OF RAIN" movie, the writer found that the character Rain (1). experiences positive symptoms, such as hallucinations and delusions. (2). The impact of schizophrenia makes it difficult for Rain to adapt to her family, friends and environment, because relapse can pose serious risks such. (3). In the film, the role of parents is very important like Rain parent’s who always accompany and support Rain in every difficult time. This shows how important the role of parents is in the condition of children with schizophrenia.The conclusion is that in Fear of Rain movie the main character experiences early symptoms in the form of hallucinations and delusions that cause Rain to experience excessive anxiety and aggressive behavior.
Estrategia para mejorar la seguridad en la prescripción de dosis altas de ácido acetilsalicílico en atención primaria
Authors: Prieto Utiel Eva , Trillo Gallo Esther , Almodóvar Carretón Mª José , Basagoiti Carreño Blanca , Herrero Domínguez-Berrueta M Carmen , Gangoso Fermoso Ana , Ibarra Mira Mª Luisa , Díez Alcántara Ana 
Published: November 2023

Objetivo: Se planteó un estudio cuyo objetivo principal fue cuantificar el cambio en el número de pacientes con edad ≥74 años en tratamiento crónico con dosis altas de ácido acetilsalicílico (AAS) (> 150 mg), después de una estrategia de intervención del farmacéutico de atención primaria (FAP).
Método: estudio transversal de intervención en pacientes ≥74 años con AAS >150 mg, dispensados en un periodo de 6 meses (enero-julio 2021), en 40 centros de salud.
Resultados: 731 pacientes ≥74 años con dosis altas de AAS (3,6%). 56,6% mujeres, edad media=85,0 años (IC 95% 84,6-85,5); media de años con AAS > 150 mg = 6,5 (IC 95% 6,2-6,8). Se consiguió una reducción de la dosis en 394 pacientes (53,9%; IC 95% 50,2 -57,6). El número total de pacientes y porcentaje en el que la aceptación de la intervención fue mayor del 50% según el diagnóstico fue: insuficiencia venosa (n=8; 87,5%), enfermedad trombótica venosa (n=4; 75,0%), diagnóstico incierto (n=56; 69,6%), fibrilación auricular (n=25; 64,0%), prevención primaria de enfermedad cardiovascular (n=102; 62,7%), arteriopatía periférica (n=22; 54,5%) y accidente cerebrovascular (n= 362; 51,7%). Un 17,2% (IC 95% 14,57-20,17) no estaban en tratamiento concomitante con un IBP.
Conclusiones: a pesar de la evidencia científica, se siguen encontrando prescripciones de AAS a dosis altas en mayores, exponiéndoles a un mayor riesgo de hemorragias. Esta estrategia ha sido eficaz ya que ha permitido adecuar la dosis a más de la mitad de los pacientes. Los FAP pueden desempeñar una importante labor en la detección y resolución de potenciales reacciones adversas.
MOTIVASI BERPRESTASI DALAM NOVEL NEGERI 5 MENARA
Authors: Syarifah Laili 
Published: November 2023

To achieve the results of a goal requires an encouragement. The power that is the driving force is called motivation, which shows a condition in the individual who drives and moves the individual to carry out an activity to achieve a goal. Motivation underlies all individual behaviors, as well as the learning process undertaken by students. Learning is a long process which has been carried out for years. Learning requires motivation, especially achievement motivation, so that it can encourage students to give their best. This type of research is library research, which is an attempt to obtain data using library resources. The types of data used in this study are primary data and secondary data. In analyzing the data that has been collected, the author uses several methods of analysis, namely descriptive methods and content analysis methods. In the novel Negeri 5, Menara Alif was able to carve out achievements by being elected as a Student Speaker. Chosen as a speaker is a long journey that is the result of rigorous selection and observation of speech and language skills. Alif's desire arose when a year before the King was chosen as a speaker when he welcomed the Egyptian Ambassador.
METODE IJTIHAD DALAM KONSTRUKSI HUKUM TAHKIM INDONESIA
Authors: Nurul Khikmah  , Karimatul Khasanah  
Published: November 2023

This paper provides input on where Tahkim or arbitration which is a form of dispute resolution outside the court and aims to reconcile the conflicting parties has existed and has long been practiced in the customs and culture of the Indonesian nation. However, with the increasing need for a peace model to resolve conflicts amid the rapid development of sharia business in Indonesia today, it is necessary to formulate a sharia tahkim model that accommodates local values. The fiqh rules clearly state that al-hukmuyadurruma’aillatihi wujudan waadaman, that the law is enforced according to ilat whether it exists or does not exist, as well as the taghayyural-ahkam bitaghayural amkinat waal-azman rule that the law changes according to changes in place and time. The type of this research is juridical philosophical with maslahah approach. The data sources used are secondary and consist of three legal materials: primary, secondary, and tertiary. The type of this research is juridical philosophical with maslahah approach. The data sources used are secondary data sources consisting of three legal materials, namely primary, secondary and tertiary. The analysis was carried out using a descriptive inductive technique. The results of this study are that several ijtihad methods such as istihsan, maslahah mursalah, and especially the technique of establishing law with 'urf can be used to realize Indonesian Tahkim fiqh. The selection of these three methods, especially the 'urf which is based on benefit and istihsan is very suitable for conditions in Indonesia, considering that a culture of peace rooted in adat is still very strong.