bluuebunny/crossref_metadata_2025_split huggingface dataset:
S-Nitrosylation-mediated coupling of DJ-1 with PTEN induces PI3K/AKT/mTOR pathway-dependent keloid formation
Authors: Lv Dongming , Xu Zhongye , Cheng Pu , Hu Zhicheng , Dong Yunxian , Rong Yanchao , Xu Hailin , Wang Zhiyong , Cao Xiaoling , Deng Wuguo , Tang Bing 
Published: December 2023

Abstract

Background
Keloids are aberrant dermal wound healing characterized by invasive growth, extracellular matrix deposition, cytokine overexpression and easy recurrence. Many factors have been implicated as pathological causes of keloids, particularly hyperactive inflammation, tension alignment and genetic predisposition. S-Nitrosylation (SNO), a unique form of protein modification, is associated with the local inflammatory response but its function in excessive fibrosis and keloid formation remains unknown. We aimed to discover the association between protein SNO and keloid formation.

Methods
Normal and keloid fibroblasts were isolated from collected normal skin and keloid tissues. The obtained fibroblasts were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The effects of DJ-1 on cell proliferation, apoptosis, migration and invasion, and on the expression of proteins were assayed. TurboID-based proximity labelling and liquid chromatography-mass spectrometry were conducted to explore the potential targets of DJ-1. Biotin-switch assays and transnitrosylation reactions were used to detect protein SNO. Quantitative data were compared by two-tailed Student’s t test.

Results
We found that DJ-1 served as an essential positive modulator to facilitate keloid cell proliferation, migration and invasion. A higher S-nitrosylated DJ-1 (SNO-DJ-1) level was observed in keloids, and the effect of DJ-1 on keloids was dependent on SNO of the Cys106 residue of the DJ-1 protein. SNO-DJ-1 was found to increase the level of phosphatase and tensin homolog (PTEN) S-nitrosylated at its Cys136 residue via transnitrosylation in keloids, thus diminishing the phosphatase activity of PTEN and activating the PI3K/AKT/mTOR pathway. Furthermore, Cys106-mutant DJ-1 is refractory to SNO and abrogates DJ-1-PTEN coupling and the SNO of the PTEN protein, thus repressing the PI3K/AKT/mTOR pathway and alleviating keloid formation. Importantly, the biological effect of DJ-1 in keloids is dependent on the SNO-DJ-1/SNO-PTEN/PI3K/AKT/mTOR axis.

Conclusions
For the first time, this study demonstrated the effect of transnitrosylation from DJ-1 to PTEN on promoting keloid formation via the PI3K/AKT/mTOR signaling pathway, suggesting that SNO of DJ-1 may be a novel therapeutic target for keloid treatment.
Perfusion management during cardiopulmonary bypass in a patient with high cardiac output due to arteriovenous malformations
Authors: Pregaldini Fabio , Makaloski Vladimir , Erdoes Gabor , Schoenhoff Florian S 
Published: December 2023

Abstract
We present a case of a 37-year-old woman diagnosed with Parkes-Weber syndrome characterized by high-flow arterio-venous malformations of the pelvic region with an estimated cardiac output of 7.2–8.5 l/min. Due to concomitant Marfan syndrome, the patient also presented an aortic root aneurysm needing surgical treatment under cardiopulmonary bypass. Concerns arose regarding the ability to maintain sufficient perfusion pressure during cardiopulmonary bypass due to the arterio-venous malformations. To address this, an intravascular balloon occlusion at the level of the aortic bifurcation was placed in order to limit arterial inflow into the arterio-venous malformations and achieve optimal perfusion pressure during cardiopulmonary bypass. The patient did not experience any complications and recovered completely after surgery.
On Being Tongue-Tied: Franchise, Fluency, and Precarity in Montaigne’s ‘De la vanité’
Authors: O’Sullivan Luke 
Published: December 2023

Abstract
Drawing attention to a key but overlooked moment in Montaigne’s ‘De la vanité’, this article interrogates the relationship between two aspects of the essayist’s ‘franchise’: his personal freedom and his frank, direct speech. It offers a reading that runs counter to Montaigne’s self-characterization as ‘le plus libre et moins endebté’ person he knows, underscoring the precarity of his ‘franchise’ and his anxieties surrounding it. In this context, ‘franchise’ is shown to be hemmed in by a cluster of obligations, even as the essayist seeks to escape them. In contrast to the critical interest in Montaigne’s reflections on free-thinkers and courageous speakers, including Socrates, Seneca and Cato the Younger, and complementing studies that have highlighted the motif of babble and loquacity in the Essais, this article focuses on Montaigne seeing himself in a frightful inversion of ‘franchise’: the looking glass of Lyncestes and the story of his death as a consequence of his disfluency. Reading ‘franchise’ in this light helps us to learn what Montaigne considered one of the first lessons worth learning: the difference between ‘liberté’ and ‘licence’.
A prospective cohort study of presenteeism and increased risk of unemployment among Japanese workers during the COVID-19 pandemic
Authors: Fujino Yoshihisa , Okawara Makoto , Hino Ayako , Muramatsu Keiji , Nagata Tomohisa , Tateishi Seiichiro , Tsuji Mayumi , Ogami Akira , Ishimaru Tomohiro ,   for the CORoNaWork project
Published: December 2023

Abstract
Objectives: Presenteeism adversely affects workers’ quality of life, leading to further deterioration of their health and affecting their ability to continue working. Unemployment is one of the most serious consequences for workers experiencing presenteeism. A worker’s ability to work depends on the degree of mismatch between their health status and job demands and work factors. The COVID-19 pandemic has affected workers’ experiences of presenteeism as well as their employment status. We examined the association between presenteeism and risk of job resignations and unemployment among Japanese workers during the COVID-19 pandemic.
Methods: A prospective study of 27 036 internet monitors was conducted, starting in December 2020, with 18 560 (68.7%) participating in the follow-up by December 2021. The Work Functioning Impairment Scale (WFun) was used to measure the degree of work function impairment.
Results: The group with the highest WFun scores had higher odds ratios (ORs) for both retirement and unemployment for health reasons than the group with the lowest WFun scores. ORs were 2.99 (95% CI, 2.48-3.62; P &lt; .001) and 1.82 (95% CI, 1.65-2.00; P &lt; .001), respectively.
Conclusions: Workers with work functioning impairment are at increased risk of resignation or unemployment. Management strategies for workers with work functioning impairment are needed to reduce their disadvantages in employment.
Using Bayesian phylogenetics to infer manuscript transmission history
Authors: McCollum Joey , Turnbull Robert 
Published: December 2023

Abstract
Bayesian phylogenetic methods offer various models that would be especially suitable in the reconstruction of textual traditions, but text-critical applications of phylogenetics to date have generally not taken advantage of these features. In this article, we offer a way forward for text-critical phylogenetics. On the side of theory, we highlight multiple Bayesian phylogenetic models and discuss their relevance to textual criticism. More practically, we show how TEI XML collations of textual traditions can be encoded to facilitate robust analyses using these models in BEAST 2, with the teiphy Python package mediating the conversion from TEI XML to BEAST XML. Finally, we give a proof of concept for this approach, showing that the results of BEAST 2 analyses of a sample collation of the Epistle to the Ephesians under different clock models cohere with established findings on the textual tradition of this work.
Spin effect on redox acceleration and regioselectivity in Fe-catalyzed alkyne hydrosilylation
Authors: He Peng , Hu Meng-Yang , Li Jin-Hong , Qiao Tian-Zhang , Lu Yi-Lin , Zhu Shou-Fei 
Published: December 2023

ABSTRACT
Iron catalysts are ideal transition metal catalysts because of the Earths abundant, cheap, biocompatible features of iron salts. Iron catalysts often have unique open-shell structures that easily undergo spin crossover in chemical transformations, a feature rarely found in noble metal catalysts. Unfortunately, little is known currently about how the open-shell structure and spin crossover affect the reactivity and selectivity of iron catalysts, which makes the development of iron catalysts a low efficient trial-and-error program. In this paper, a combination of experiments and theoretical calculations revealed that the iron-catalyzed hydrosilylation of alkynes is typical spin-crossover catalysis. Deep insight into the electronic structures of a set of well-defined open-shell active formal Fe(0) catalysts revealed that the spin-delocalization between the iron center and the 1,10-phenanthroline ligand effectively regulates the iron center's spin and oxidation state to meet the opposite electrostatic requirements of oxidative addition and reductive elimination, respectively, and the spin crossover is essential for this electron transfer process. The triplet transition state was essential for achieving high regioselectivity through tuning the nonbonding interactions. These findings provide an important reference for understanding the effect of catalyst spin state on reaction. It is inspiring for the development of iron catalysts and other Earth-abundant metal catalysts, especially from the point of view of ligand development.
Machine learning reveals that climate, geography, and cultural drift all predict bird song variation in coastal Zonotrichia leucophrys
Authors: Yang Jiaying , Carstens Bryan C , Provost Kaiya L 
Published: December 2023

Abstract
Previous work has demonstrated that there is extensive variation in the songs of White-crowned Sparrow (Zonotrichia leucophrys) throughout the species range, including between neighboring (and genetically distinct) subspecies Z. l. nuttalli and Z. l. pugetensis. Using a machine learning approach to bioacoustic analysis, we demonstrate that variation in song is correlated with year of recording (representing cultural drift), geographic distance, and climatic differences, but the response is subspecies- and season-specific. Automated machine learning methods of bird song annotation can process large datasets more efficiently, allowing us to examine 1,913 recordings across ~60 years. We utilize a recently published artificial neural network to automatically annotate White-crowned Sparrow vocalizations. By analyzing differences in syllable usage and composition, we recapitulate the known pattern where Z. l. nuttalli and Z. l. pugetensis have significantly different songs. Our results are consistent with the interpretation that these differences are caused by the changes in characteristics of syllables in the White-crowned Sparrow repertoire. This supports the hypothesis that the evolution of vocalization behavior is affected by the environment, in addition to population structure.
Social Movements
Authors: Chua Lynette J. 
Published: December 2023

Abstract
Although social movements and constitutional law are closely connected, their relationship tends to be overlooked in Asian constitutional law scholarship. Drawing from the socio-legal subfield of law and social movements, this chapter highlights the significance of studying social movements and their relationship to constitutional law in Asia. Because Asian jurisdictions are diverse, running the gamut from liberal democracies to various shades of illiberalism, this chapter does not emphasize any shared characteristics among social movements in the region. Rather, it considers social movement emergence, strategies and tactics, and both litigation and non-litigation outcomes in its examination of the relationship between social movements and constitutional law. It concludes with lessons from the socio-legal study of law and social movements, as well as from legal scholarship that has been influenced by this field, to offer suggestions on how Asian constitutional law scholars could approach the study of movement-constitution dynamics.
Psychology in Mathematics Education
Authors: Jones Ryan Seth , Lovett Jennifer , Fletcher Samantha , Heath Amanda Lake 
Published: December 2023

Abstract
This chapter explores the influence of psychology on Mathematics Education research, which is motivated by a desire to improve mathematical thinking and learning and coordinated between mathematics, psychologists, and educators. It explains how psychological perspectives are widely influential across research programs, especially depending on the age of the participants in the context. Moreover, the perspective ranges between diverse theoretical orientations from sociology, anthropology, economics, and many other philosophies. Mathematics Education researchers employ a diverse array of theoretical and methodological tools and often conduct research with different goals in mind. The chapter then highlights the importance of coordinating diverse perspectives for the future of Mathematics Education.
Precision medicine in inflammatory bowel disease
Authors: Zeng Zhen , Jiang Mingshan , Li Xi , Yuan Jing , Zhang Hu 
Published: December 2023

Abstract
Inflammatory bowel disease (IBD) is an incurable disease characterized by remission-relapse cycles throughout its course. Both Crohn's disease (CD) and ulcerative colitis (UC), the two main forms of IBD, exhibit tendency to develop complications and substantial heterogeneity in terms of frequency and severity of relapse, thus posing great challenges to the clinical management for IBD. Current treatment strategies are effective in different ways in induction and maintenance therapies for IBD. Recent advances in studies of genetics, pharmacogenetics, proteomics and microbiome provide a strong driving force for identifying molecular markers of prognosis and treatment response, which should help clinicians manage IBD patients more effectively, and then, improve clinical outcomes and reduce treatment costs of patients. In this review, we summarize and discuss precision medicine in IBD, focusing on predictive markers of disease course and treatment response, and monitoring indices during therapeutic drug monitoring.
Forearm Granular Cell Tumor: A Rare Entity
Authors: Mansour Walaa Anis , El Saloussy Yasmeen , Wahdan Wessam 
Published: December 2023

Summary:
Granular cell tumors are uncommon benign masses. They occur rarely and are usually solitary, nontender lesions, with a tendency to occur in the head and neck region and a predilection for the tongue. A malignant variant is even less common. To date, its origin remains uncertain. We report the case of a 19-year-old Egyptian man who presented to the Kasr eini Plastic Surgery Clinic with a swelling on the posterior aspect of his right forearm. After surgical excision and histopathological examination, it was found to be a granular cell tumor. This finding was of interest because of the rarity of the neoplasm in general, but in particular because of the scarcity of its occurrence in this anatomic location and the patient’s sex, and age group.
Potential of serious games as a competency assessment tool for acute care nurses on the blood transfusion procedure
Authors: Rajasegeran Darshini Devi , Liu Kai , Sheng Yanyan , Loh Christian Sebastian , Choh Andrea Chau Lin , Teo Kai Yunn , Fan Peijin Esther Monica , Tan Min Yi , Aloweni Fazila , Ang Shin Yuh 
Published: December 2023

Background:
Blood transfusion is a life-saving treatment that requires comprehensive knowledge and compliance to safety, administration, and management of blood transfusion process. It is a core competency for a registered nurse. Competency assessments are annually conducted as regulatory requirement and for quality assurance within our institution. Prior to using serious games, competency assessment involved an online learning module followed by a face-to-face assessment. This had limitations such as such as limited opportunities, potential lack of standardisation, time, and resources.

Aim:
The aim of this study was to assess whether serious game for blood transfusion administration could be a valid and reliable nursing competency assessment tool through capturing and analysis of in-game empirical data.

Methods:
The nursing department from Singapore General Hospital (SGH) developed a serious game for blood transfusion administration. The game modules were developed to match the actual procedure with established learning objectives at each stage. The game’s content validity was assessed by four external subject-matter-experts. Gameplay data of 1093 nurses were tested for construct validity, internal-consistency reliability, and criterion validity of the game.

Results:
The item-level content validity index (I-CVI) ranged between 0.75 and 1, while the average of the I-CVI scores for all items on the scale (S-CVI/Ave) was 0.99. Six factors and 82 game actions were obtained using principle factor analysis. Most game actions had a communality value (h2) above 0.5. The Inter-factor correlations between the six factors ranged from 0.2 to 0.45. Internal-consistency reliability estimates (α) (together with their 95% CIs) for the stages were greater than 0.75, with an overall of 0.96 for the 82 game actions. Linear association between each predictor and the criterion were below 0.25.

Conclusion:
Based on the empirical data collated, we conclude that the serious game may be a valid competency assessment tool for nurses in SGH. For future work, we propose to conduct the blood transfusion serious game in other hospitals to further support its validity and reliability as a competency tool for multi-step complex procedural clinical skills.
Monitoring for 5-aminosalicylate toxicity: prognostic model development and validation
Authors: Abhishek A , Nakafero Georgina , Grainge Matthew J , Card Tim , Taal Maarten W , Aithal Guruprasad P , Fox Christopher P , Mallen Christian D , Stevenson Matthew D , Riley Richard D 
Published: December 2023

Background and aimTo develop and validate a prognostic model for risk-stratified monitoring of 5-aminosalicylate (5-ASA) toxicity.MethodsThis nationwide retrospective cohort study used data from the Clinical Practice Research Datalink (CPRD) Aurum and Gold for model development and validation respectively. It included adults newly diagnosed with inflammatory bowel disease (IBD) and established on 5-ASAs between 01/01/2007 and 31/12/2019. 5-ASA discontinuation with abnormal monitoring blood test result was the outcome of interest. Patients prescribed 5-ASAs for ≥6 months i.e., established on treatment, were followed-up for up to five years. Penalised Cox-regression was used to develop the risk equation. Model performance was assessed in terms of calibration and discrimination. Statistical analysis was performed using STATA (StataCorp LLC).Results14,109 and 7,523 participants formed the development and validation cohorts with 401 and 243 events respectively. 185, 172, and 64 discontinuations were due to cytopenia, elevated creatinine and elevated liver enzymes respectively in the derivation cohort. Hazardous alcohol intake, chronic kidney disease, thiopurine use, and blood test abnormalities before follow-up were strong prognostic factors. The optimism adjusted R2Din development data was 0.08. The calibration slope and Royston D statistic (95% Confidence Interval) in validation cohort were 0.90 (0.61-1.19) and 0.57 (0.37-0.77) respectively.ConclusionThis prognostic model utilises information available during routine clinical care and can be used to inform decisions on the interval between monitoring blood-tests. The results of this study ought to be considered by guideline writing groups to risk-stratify blood test monitoring during established 5-ASA treatment.What is already known?Renal, hepatic and blood toxicity are uncommon during long-term 5-aminosalicylate (5-ASA) treatment.There are no mechanisms to predict the risk of these toxicities during established treatment that may be used to risk stratify blood-test monitoring.What this study adds?Using a large national dataset originated during routine care, this study developed a prognostic model that discriminated patients at varying risk of 5-ASA toxicity during established treatment with good performance characteristics validated.Most patients were at low-risk of toxicity due to 5-ASAs and could continue with annual monitoring blood-tests while others at high risk may require more frequent monitoring.This prognostic model can be used to make an informed decision on the interval between monitoring blood tests and the findings ought to be considered by guideline writing groups to bring about equitable and sustainable change in clinical practice.
Comparative Analysis of the Duodenojejunal Microbiome with the oral and fecal microbiome unveils its role in Human Severe Obesity
Authors: Steinbach Emilie , Belda Eugeni , Alili Rohia , Adriouch Solia ,   Jejunal Luminal and Colonic Mucosa-Associated Microbiota in Metabolic Diseases (Je/Col-MiMe) Group, Chassaing Benoit , Le Roy Tiphaine , Clement Karine 
Published: December 2023

AbstractThe intestinal microbiota is recognized as an important player in the development and maintenance of obesity. Most studies focus on faecal microbiota because of its accessibility. However, the small intestine is a major site for nutrient sensing and absorption and few studies have examined the composition and function of the microbiota in this segment of the digestive tract.We conducted a clinical research project on 30 age- and sex-matched participants with (N=15) and without (N=15) obesity. Duodenojejunal fluid was obtained by aspiration during fibroscopy. Phenotyping included clinical variables related to metabolic status, lifestyle and psychosocial factors using validated questionnaires. Metagenomic analyses of the oral, duodenojejunal and faecal microbiome, as well as metabolomic data from duodenojejunal fluid and faeces, were integrated with clinical and lifestyle data.The results show associations between duodenojejunal microbiota and lifestyle as well as clinical phenotypes. These associations had larger effect sizes than the associations between these variables and faecal microbiota. We also observed that the duodenojejunal microbiota of obese patients had a higher diversity. In addition, we observed differences in the abundance of several species of the duodenojejunal microbiota between control individuals and patients suffering from obesity.In conclusion, our results support the relevance of studying the role of the small intestinal microbiota in the development of metabolic diseases.