bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improvement of the Oxidation Stability of Waste Cooking Oil Biodiesel Using Natural Antioxidants
Authors: Kongolo Emmanuel , Jager Debbie D , Ameh Alechine E , Oyekola Oluwaseun O. 
Published: November 2023

Abstract
Oxidation degradation is one of the primary obstacles hindering the commercialization of biodiesel, which has been established as a viable alternative to diesel derived from fossil fuels. In the present study, the Rancimat method is employed to determine the efficacy of three natural antioxidants, vitamins A, C, and E, in enhancing the oxidation stability of biodiesel produced from waste cooking oil (WCO). At a concentration of 1000 ppm, the selected antioxidants improved the oxidation stability of biodiesel. Vitamin C improved the WCO biodiesel induction period (IP) from 0.79 to 7 hours most effectively. This is due to the low bond dissociation energy (BDE, 318.5 kJ/mol), the low molecular weight (Mw, 176.16 g/mol), and the formation of acetyl palmitate, which is known to possess antioxidant properties in oils. When antioxidants A and C (IP = 12.9 hours) and antioxidants C and E (IP = 7 hours) were combined in a ratio of 1:1, they were more effective. The combination of A and E negatively affected the oxidation stability of WCO biodiesel, resulting in an induction period of 0.33 hours. Herein, the present research has demonstrated that the utilisation of antioxidant C, either alone or in conjunction with other natural antioxidants, exerts a favourable impact on the oxidative stability of WCO biodiesel.
A case of tongue swelling after S-1, oxaliplatin and trastuzumab for HER2-positive gastric cancer
Authors: abe taichi , Sumiya Tatsuya , Tsuji Ayaka , Hanai Izumi , Otomo Yukiko , Yamamoto Takae , Iguchi Emiko 
Published: November 2023

Abstract
Background
We report a case of a patient with HER2-positive gastric cancer with marked tongue swelling during the second cycle of S-1 and oxaliplatin, trastuzumab.
Case Presentation:
The patient was a 74-year-old male, who was taking ARB for pre-existing hypertension, no history of allergies, diagnosed with HER2-positive gastric cancer, treated with S-1 and oxaliplatin for the first cycle, and trastuzumab was added from the second cycle. Three weeks after initiation, during an outpatient visit, grade 2 oral mucositis and significant enlargement of the patient's tongue were observed. Due to the risk of airway obstruction, the patient was referred to an otolaryngologist. After examination, hereditary angioedema was ruled out, and treatment was discontinued in view of ARB-induced angioedema. However, the tongue swelling did not improve markedly. Considering disease progression due to the discontinuation of chemotherapy, it was decided to change S-1 to capecitabine and continue treatment, and chemotherapy was continued.
Conclusions
Angioedema has been reported to be hereditary and drug-related, and ACE inhibitors and ARBs have also been reported as drug-related. Since the patient had oral mucositis at the time of onset and was taking an ARB, it is thought that SOX and trastuzumab during ARB therapy-induced oral mucositis, leading to the development of angioedema.
EPB41L4A-AS1 modulates Aβ clearance by regulating autophagy-related gene expression
Authors: wang ziqiang , Wang Ruomei , Niu Lixin , Zhou Xiaoyan , Han Jinxiang , Li Kun 
Published: November 2023

Abstract

Alzheimer's disease (AD) is the most common neurodegenerative disorder characterized by the deposition of β-amyloid (Aβ) plaques. Aβ is generated from the cleavage of the amyloid precursor protein by β and γ-secretases and cleared by neuroglial cells mediated autophagy. The imbalance of the intracellular Aβ generation and clearance is the causative factor for AD pathogenesis. However, the exact underlying molecular mechanisms remain unclear. Our previous study reported that EPB41L4A-AS1 is an aging-related long non-coding RNA (lncRNA) that is repressed in patients with AD. In this study, we found that downregulated EPB41L4A-AS1 in AD inhibited neuroglial cells mediated-Aβ clearance by decreasing the expression levels of multiple autophagy-related genes. We found that EPB41L4A-AS1 regulates the expression of general control of amino acid synthesis 5-like 2, an important histone acetyltransferase, thus affecting histone acetylation, crotonylation, and lactylation near the transcription start site of autophagy-related genes, ultimately influencing their transcription. Collectively, this study reveals EPB41L4A-AS1 as an AD-related lncRNA via mediating Aβ clearance and provides insights into the epigenetic regulatory mechanism of EPB41L4A-AS1 in gene expression and AD pathogenesis.
Digital technological innovation and clinical risk policy: results and prospects in an Italian private hospital
Authors: Pesel Giuliano , Gibelli Filippo , Bailo Paolo , Ricci Giovanna 
Published: November 2023

Abstract

Background
.The healthcare industry is witnessing a transformative era marked by the advent of digital technologies. One such notable development is the digitization of prescription therapy, which holds potential for significant clinical risk reduction. The traditional methods of maintaining health records, particularly in regions like Italy, are predominantly paper-based, presenting challenges like poor legibility and transcription errors, leading to possible medication mishaps and adverse events. The advent of electronic medical record models, as seen in the private healthcare facility in Friuli Venezia-Giulia, north-eastern Italy, presents a promising solution to these issues.
Methods
.This study explores the implementation of the Electronic Unified Therapy Record (EUTR), a digital tool initiated with the partial digitization of the clinical folder. The EUTR, operational since 2021, is an application that consolidates all therapy sheet information and supplements it with patient-specific data. It is employed during the prescription phase by doctors and the administration phase by nurses, aiming to reduce assistance times and mitigate errors associated with incorrect drug administration.
Results
The preliminary outcomes of implementing the EUTR have shown significant potential in enhancing safety and quality of care. By digitizing and centralizing patient treatment data, EUTR ensures accuracy and consistency, thereby enhancing patient safety. Its real-time updating feature allows for instant error detection, swift rectification, and seamless communication among healthcare professionals. These features have contributed to a reduction in assistance time and a decrease in therapeutic errors.
Conclusions
The introduction of digital tools like the EUTR in healthcare is a promising step towards reducing clinical risks and enhancing patient care. While the preliminary outcomes have been positive, the study concludes with a proposal for future enhancements to the digital tool. Continued innovation in healthcare digitalization, driven by tools like EUTR, can potentially revolutionize patient care, paving the way for a safer, more efficient, and patient-centered healthcare system. This study serves as a benchmark for further research on the impact of digital transformation in healthcare.
Improving Response Time using Lightning based Biased Random Sampling (LBRS) for Load Balancing
Authors: evangeline preetha , D Preetha Evangeline , P Anandhakumar , V Sathya , P Karthikeyan 
Published: November 2023

Abstract
Cloud computing has rapidly emerged as a successful paradigm for providing IT infrastructure, resources and services on a pay-per-use basis over the past few years. The wider adoption of Cloud has led to the establishment of large scale data centers that consume excessive energy. Biased Random Sampling is an existing load balancing algorithm used to choose the load allocation node from the neighboring nodes to satisfy the user requests. Proposed work applies the behavior of lightning to Biased Random Sampling algorithm which is used for load balancing technique in cloud to achieve minimum response time. The work also computes the capacity of the machine to achieve high accuracy in calculating response time while assigning cloudlet received to VM. The work also includes obtaining resources from public cloud when load increases in the private cloud. Response time of proposed work is compared with existing biased random sampling and minimum response time is obtained for proposed load balancing technique. The response time obtained in LBRS is 82% less than BRS algorithm and 74% less than FCFS algorithm. The experimental results showed that the load is evenly distributed for LBRS algorithm and unevenly distributed for BRS algorithm.
LRRK2 regulates ferroptosis through the system Xc-GSH-GPX4 pathway in the neuroinflammatory mechanism of Parkinson's disease
Authors: Lu Guohui , Zheng Zijian , Zhang Shushan , Liu Xinjie , Wang Xiangrong , Xue Cheng , Wu Xiao , Zhang Xinran , Liu Zheng , Yao Longping 
Published: November 2023

Abstract
Parkinson's disease (PD) is the most prevalent neurodegenerative disorder. Neuroinflammation mediated by activated microglia and apoptosis of dopaminergic (DA) neurons in the midbrain are its primary pathological manifestations. Leucine-rich repeat protein kinase 2 (LRRK2) kinase has been observed increased expression during neuroinflammation. however, the effect of LRRK2 on microglia activation remains poorly understood. In this study, we have established lipopolysaccharide (LPS) treated BV2 cells and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) models for both in vivo and in vitro investigation. Our data in vivo reveal that LRRK2 can promote microglia activation by regulating ferroptosis and activating NF-κB. Inhibition of LRRK2 expression effectively suppressed the LPS-induced pro-inflammatory cytokines and facilitated the secretion of neuroprotective factors. Importantly, we identified the system Xc-GSH-GPX4 pathway as a critical factor in LRRK2-mediated microglial inflammatory response via the regulation of ferroptosis. Using a microglial culture supernatant (MCS) transfer model, we found that inhibiting LRRK2 or downregulating ferroptosis in BV2 cells prevented SH-SY5Y cell apoptosis. Additionally, we observed abundant expression of LRRK2 and p-p65 in the midbrain, which was elevated in the MPTP-induced PD model, along with microglia activation. LRRK2 and p-p65 expression inhibition with PF-06447475 attenuated microglia activation in the nigrostriatal dense part of MPTP-treated mice. Based on our findings, it is evident that LRRK2 plays a critical role in promoting the neuroinflammatory response during the pathogenesis of PD by regulating the system Xc-GSH-GPX4 pathway. Taken together, our data highlights the potential research and therapeutic value of targeting LRRK2 to regulate neuroinflammatory response in PD through ferroptosis. .
First severe malaria in pregnancy followed in Philippine real-world setting: proof-of-concept of probabilistic record linkage between disease surveillance and hospital administrative data
Authors: Kinoshita Takuya , Espino Fe Esperanza Caridad , Bunagan Raymart , Lim Dodge , Daga Chona , Parungao Sabrina , Balderian Aileen , Micu Katherine , Laborera Rutchel , Basilio Ramon , Inobaya Marianette , Baquilod Mario , Dy Melecio , Chiba Hitoshi , Matsumoto Takehiro , Nakayama Takeo , Kita Kiyoshi , Hirayama Kenji 
Published: November 2023

Abstract
Background
Although the Philippines targets malaria elimination by 2030, it is a disease of public health importance in provinces that report malaria. Pregnant women residing in endemic areas are a vulnerable population because their pregnancy is not followed through, and the outcome of their pregnancy is unknown. This study determined the utility of real-world data integrated with disease surveillance dataset as real-world evidence of pregnancy and delivery outcomes in areas endemic for malaria in the Philippines.
Methods
For the period of 2015 to 2019, electronic datasets of malaria surveillance data and Ospital ng Palawan hospital admission log of pregnant women residing in the four selected barangays of Rizal, Palawan were merged using probabilistic linkage. The source data for record linkage were first and last names, birth date, and address as the mutual variable. The data used for characteristics of the pregnant women from the hospital data set were admission date, discharge date, admitting and final diagnosis and body weight on admission. From the malaria surveillance data these were date of consultation, and malaria parasite species. The Levenshtein distance formula was used for a fuzzy string-matching algorithm. Chi-square test, and Mann-Whitney U test were used to compare the means of the two datasets.
Results
The prevalence of pregnant women admitted to the tertiary referral hospital, Ospital ng Palawan, was estimated to be 8.34/100 overall, and 11.64/100 from the four study barangays; that of malaria during pregnancy patients was 3.45/100 and 2.64/100, respectively. There was only one true-positive matched case from 238 women from the hospital and 54 women from the surveillance datasets. The overall Levenshstein score was 97.7; for non-matched cases, the mean overall score was 36.6 (35.6–37.7). The matched case was a minor who was hospitalized for severe malaria. The outcome of her pregnancy was detected from neither dataset but from village-based records.
Conclusion
This proof-of-concept study demonstrated that probabilistic record linkage could match real-world data in the Philippines with further validation required. The study underscored the need for more integrated and comprehensive database to monitor disease intervention impact on pregnancy and its outcome in the Philippines.
Nicotinamide protects against diabetic kidney disease through regulation of Sirt1
Authors: Yang Yeping , Huang Jinya , Xie Lijie , Wang Yilin , Guo Shizhe , Wang Meng , Shao Xiaoqing , Liu Wenjuan , Wang Yi , Li Qin , Wu Xia , Zhang Zhaoyun , Zeng Fangfang , Gong Wei 
Published: November 2023

Abstract
Purpose
To investigate the effect of nicotinamide (Nam) on diabetic kidney disease (DKD) in mice and explore its mechanism.
Methods
Thirty DBA/2J mice were randomly assigned to three groups. After 8 weeks of hyperglycemia induced by streptozocin (STZ), Nam and saline were administrated to the STZ + Nam and STZ + NS mice, respectively, for 8 weeks. Non-diabetic mice (NDM) were used as control group. Twenty In2−/− Akita mice were randomly divided into two groups. After 8 weeks of hyperglycemia, Nam and saline were administered to Akita + Nam and Akita + NS mice, respectively, for 6 weeks. Wild-type littermates were used as the control group. Markers of renal injury were analyzed, and the molecular mechanisms were explored in human proximal tubular HK2 cells.
Results
Urinary albumin-to-creatinine ratio (UACR) and kidney injury molecule 1 (KIM-1) decreased in the STZ + Nam and Akita + Nam groups. Pathological analysis showed that Nam improved the structure of glomerular basement membrane, ameliorated glomerular sclerosis, and decreased the accumulation of extracellular matrix and collagen. Compared to the diabetic control group, renal fibrosis, inflammation, and oxidative stress were reduced in the Nam-treated mice. The expression of sirtuin 1 (SIRT1) in human proximal tubular HK2 cells was inhibited by high glucose and Nam treatment enhanced its expression. However, in HK2 cells with SIRT1 knockdown, the protective effect of Nam was abolished, indicating that the beneficial effect of Nam was partially dependent on SIRT1.
Conclusions
Nam has a renoprotective effect against renal injury caused by hyperglycemia and may be a potential target for the treatment of DKD.
Can Posttransplant Cycloposphamide Reduce the Risk of Graft vs Host Disease for Pediatric Fully Matched Non-Related Stem Cell Transplantation?
Authors: Aksoy Basak Adakli , Ersoy Gizem Zengin , Oner Ozlem Basoglu , Aydogdu Selime , Dikme Gurcan , erdem melek , Fisgin Tunc , Bozkurt Ceyhun 
Published: November 2023

Abstract
Objective
Post-transplant cyclophosphamide (postCy) is widely used as prophylaxis for graft-versus-host disease (GvHD) in haploidentical bone marrow transplantation and is being investigated in matched transplants for pediatric patients. The effect of postCy for prophylaxis of GvHD in pediatric, fully-matched, unrelated, peripheral blood stem cell transplantation (PBSCT) is described.
Methods
This was a retrospective clinical study of 110 pediatric patients diagnosed with malignant and non-malignant diseases who underwent PBSCT from matched unrelated donors (MUD). Fifty patients received 50 mg/kg/d cyclophosphamide on day +3 and +4, designated Group 1, while the remaining 60 (Group 2) did not receive postCy. All patients were given a regimen of standard immunosuppression.
Results
One hundred and ten patients younger than 18 years (73 males and 37 females) were followed-up for a median of two years after successful PBSCT. The incidence of grade II–IV and grade III–IV acute GvHD was 28% and 18% in Group 2 while in Group 1 these rates were 20% and 10%, respectively. The incidence of chronic GvHD was 8.3% for Group 2 and 4% in Group 1. Survival for >100 days was 87.2% overall, 86% in Group 1 and 89% in Group 2; similarly, overall survival in these three populations was 78%, 76% and 80%, respectively. None of these differences were significant.
Conclusion
Although postCy-transplant was shown to have advantages for unmatched and haploidentical PBSCT in adult patients, the findings of the present study suggest no effect in fully MUD PBSCT in children. There is a need for larger studies to confirm or refute these findings and, if shown to be robust, to investigate why there is this difference between pediatric and adult transplant patients.
Increased epithelial cell spreading by histatin-1 is associated by enhanced mitochondrial and cytoskeletal dynamics
Authors: Bikker Floris , Ma Dandan 
Published: November 2023

Abstract
Histatin-1, a histidine-rich salivary peptide, promotes cell spreading and cell-surface adhesion thereby playing a role in faster oral wound healing processes when compared to the skin. However, the underling mechanisms remains relatively understudied. This study explored the potential relationships between histatin-1(Hst1), mitochondria and alterations in the cytoskeleton. Live cell confocal microscopes were adopted to capture the images and videos to assess the effect of Hst1 on the interaction between mitochondrial and cytoskeletal dynamics. Mitochondrial bioenergetics were determined using Seahorse® XF96 analyzers. Hst1 significantly increase filopodia formation and elongation within 24hours. NaN3, an inhibitor of cytochrome oxidase, resulted in reduced filopodial formation capacity of Hst1. This difference was likely due to distinct patterns of mitochondrial morphology, density, and membrane potential, as well as ATP production. Meanwhile, Hst1 also targeted to the actin and may involve in the process of action polymerization in cell spreading. Hst1 promotes interaction between mitochondria and cytoskeleton to induce filopodial formation preceding cell spreading.
Prevelance of Long Acting Contraceptive Use and Associated Factors Among Married Women of Reproductive Age in Angacha Destrict, Kemebata Temebaro Zone, Snnpr, Ethiopia.
Authors: Demilo Melkamu Gebre , Kassa Dejene Hailu 
Published: November 2023

Abstract
Back ground
In many developing countries including Ethiopia, rapid population growths is a major challenge to meeting the Millennium Development Goals (MDGs) and affects maternal health. Despite their safety and effectiveness, only 15.5% and 3.4% of women are globally using long acting contraceptive methods (LACMs) such as IUCDs and sub dermal implants, respectively. Angacha District has been exerting a lot of efforts to promote LACM, but there is lack of scientific- evidence regarding the status of LACM use and associated factors among married women.
Methods
A sample of 632 married women of reproductive age was participated. Multi-stage sampling technique was carried out to select the study population. First, all the 19 kebeles of the woreda were stratified into 2 urban and 17 rural kebeles. Then, 1 kebele from urban and 4 kebeles from rural, a total of 5 kebeles were randomly selected by lottery method. Data were collected by face-to-face interviews with married women of reproductive age from selected households. Descriptive and binary logistic regressions were done to describe and examine association between outcome and explanatory variables.
Results
The prevalence of LACMs use was 14.2%. Women aged between 25–34 and 35–49 years [AOR = 0.36(95%CI:0.17,0.77)] and [AOR = 0.28(95%CI:0.1,0.77)], monthly average income[AOR = 0.26(95%CI:0.1,0.69)], overall knowledge scores about LACMs [AOR = 3.1 (95%CI:1.23,7.66)], partners discussion[AOR = 0.24(95%CI:0.07,0.9)], womens’ desire for more children [AOR = 4.32(95%CI:1.55,12.03)] were significantly associated with LACMs use.
Conclusion
LACMs use in the study area was low. Some socio-demographic, economic & cultural factors like age of respondents, households’ monthly average income, overall knowledge scores about LACMs, partner’s discussion & desire for more children were associated with LACMs use.
Growth charts for children aged 6-17 years in Shanxi, China: comparison with other cities of China, WHO and neighboring countries
Authors: li Kunkun , Cao wenjun , Tao Mengmeng , Tian Wei , Chang Ruimin 
Published: November 2023

Abstract
Background
Growth charts are an important method for evaluating a child’s health, growth, and nutritional status. To present body mass index (BMI)-for-age references reflecting children’s growth in Shanxi.
Methods
A stratified cluster random sampling method was used to recruit 5461 children and adolescents aged 6–17 years. Height and weight were measured and BMI was calculated. The LMS method was used to calculate the percentile values of body mass index by gender and age. Smoothed BMI-for-age growth curves were established for both sexes and compared with reference data from other cities of China, WHO and neighboring countries.
Results
BMI centiles increased with age but with different patterns in both boys and girls. The centile curves from the 3rd to the 50th had a slight increase, while a sharp increase was seen from 11 to 17 years in boys and from 6 to 14 years in girls in the higher centiles. In comparison with other cities of China, the values for the 50th percentile are higher than those reported for children from China 2009, Shanghai, Changsha and China 2010 in both sexes. In comparison with WHO growth references, Chinese girls and boys had higher values in all percentiles, whereas curves of girls look roughly the same. The medians for BMI in Shanxi increase linearly from 6 to 17 years in boys. Compared to the neighboring countries, the 50th percentile curves of Japan, India and Iran are lower for boys and girls aged 5–14 than Korea, Shanxi and Pakistan. Shanxi has the highest percentile curves for girls and boys aged 12–17 compared to other neighboring countries.
Conclusions
The BMI percentiles of children aged 6–17 years in Shanxi differed significantly from the growth reference curves of other cities of China, WHO and neighbouring countries. Therefore, BMI curves that meet local and population-specific should be developed to assess physical growth of children, detect overweight and obese children early, and provide scientific basis for the prevention and control of overweight and obesity in children.