bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peranan Bidan Desa dalam menangani dan merujuk kasus bayi baru lahir asfiksia di Kabupaten Cirebon
Authors: Umniyati Helwiah 
Published: December 2023

Each year 3 million newborns die during the first seven days of life, accounting for 75% of all neonatal deaths. At least 1 million babies die during their first 24 hours of life. Three major causes of neonatal death are infections (pneumonia, diarrhea, and tetanus) 36%, preterm birth 28% and asphyxia 23%. Causes of death vary between the early and late neonatal periods, with deaths caused by preterm birth, asphyxia and congenital defects occur predominantly during the frst week of life and infection is the major cause of neonatal deaths thereafter. In Indonesia based on 2001 Household Health Survey (SKRT 2001) neonatal death caused by asphyxia was 27% which was the second cause of neonatal death after low birth weight. The objective of this study was to learn the role of midwives in handling and referring birth asphyxia in Cirebon District by using qualitative method. The result showed that many people living in villages in Cirebon district did not understand the causes of asphyxia. They thought that babies could not cry because of bad spirit or devil. The midewives were capable of handling the asphyxia babies according to their knowledges obtained from training about asphyxia management. Sometimes however, the midwives encountered some constrains in practice such as low birth weight babies (only 1,4 Kg) and oversize concave cover for resuscitation. Other limiting factors occurred when the midwives would like to refer the babies to the hospital. The families might refuse with some reasons such as, no trust in the medical staff, shortage of money or difficulty in finding transportation. Lack of incubator in the hospital was also another obstacle.
The Effect of Calorie Restriction on Body Weight, Fat Tissue Weight and the Histopathological Picture of Fat Tissue in Rats
Authors: Fitriyana Lena , Mukhtar Diniwati , Royhan Aan 
Published: December 2023

Background : Calorie restriction is a reduction in calorie intake without malnutrition, as the main tool to improve health (Tomiyama et al.,2017). Limitation of calorie intake around 20-40% of total calorie intake, but intake remains adequate to reduce inflammation (Trepanowski et al., 2010). Obesity is one of the consequences of excessive calorie consumption which causes inflammation so that it can increase the risk of degenerative diseases (Susantiningsih, 2015). Overweight is divided into two, namely hypertrophy occurs by the accumulation of large amounts of fat in unilocular fat cells, so that the size of fat cells reaches four times larger, but the number of fat cells does not increase. And hyperplasia is a heavy obesity because in addition to growing cells also the number of cells becomes more (Sunny Wangko, 2014). One effort that can be done to reduce the increase in degenerative diseases is by limiting caloric intake or appropriate calorie restriction (Brzek et al., 2012).Purpose : This study purpose to determine changes in body weight, fat tissue and histopathological features of fat tissue in rats given calorie restriction and reviewed according to Islamic views.Methods : This type of research is an experimental laboratory for 6 weeks with research subjects male white rats (Wistar Strain) which is divided into 2 groups, namely control, and Calorie Restrictions Taking samples of body weight and fat tissue in the abdomen and supraclavicular to see the number of cells fat, fat cell size and weight of white fat tissue and brown fat tissue.Results : Calorie restriction can reduce fat cell size, and weight of white fat tissue significantly (p <0.05). in brown fat there was a significant increase. but in the measurement of body weight and fat cell numbers there were no significant differences (p> 0.05).Conclusion : In calorie restriction there was no significant relationship after a study of body weight and fat cell count, but found a significant relationship to the weight of white fat tissue and brown fat tissue and the size of fat cells. In Islamic view calorie restriction is the same as limiting food intake as recommended by the Prophet about 1/3 of the stomach for food.
Pengaruh Polusi Udara Terhadap Berat Badan, Berat Jaringan Lemak Dan Gambaran Histopatologi Jaringan Lemak Pada Tikus
Authors: Irwansyah Febri , Mukhtar Diniwati , Zuhroni Zuhroni , Royhan Aan 
Published: December 2023

Latar Belakang : Obesitas dapat meningkatkan resiko berbagai macam penyakit vaskuler. Polusi udara dapat mempengaruhi regulasi hormon yang mengatur nafsu makan dan metabolisme energi melalui berbagai mekanisme aksi. Mekanisme ini menyebabkan ekspansi jaringan lemak berupa hipertrofi dan hiperplasi sel lemak yang menyebabkan peningkatan berat badan. Dalam pandangan Islam obesitas merupakan hal yang tidak dianjurkan, bahkan tidak disukai oleh Allah dan Rasul-Nya.Tujuan : Penelitian ini bertujuan untuk mengetahui perubahan berat badan, jaringan lemak dan gambaran histopatologi jaringan lemak pada tikus yang diberi paparan polusi udara dan ditinjau menurut pandangan islam.Metode : Jenis penelitian yang dilakukan adalah eksperimental laboratorium selama 6 minggu dengan subjek penelitian tikus putih jantan (Rattus norvegicus) yang dibagi ke dalam 2 kelompok, yaitu kontrol dan paparan polusi udara. Pengambilan sampel berat badan dan jaringan lemak pada bagian abdomen dan supraklavikula untuk melihat jumlah sel lemak, ukuran sel lemak dan berat jaringan lemak putih dan jaringan lemak coklat. Hasil : Paparan polusi udara dapat meningkatkan ukuran sel lemak, jumlah sel lemak dan berat jaringan lemak putih dan coklat secara signifikan (p<0.05). Tetapi pada pengukuran berat badan, tidak didapatkan perbedaan yang bermakna (p>0,05). Kesimpulan : Paparan polusi udara dapat meningkatkan diameter sel lemak putih, jumlah sel lemak putih, dan berat jaringan lemak putih dan lemak coklat secara signifikan. Tetapi paparan polusi udara tidak memberikan pengaruh yang berarti terhadap berat badan tikus.
Gut Microbiota in Stunted Children
Authors: Hervina Hervina , Ernawati Kadek Lusi , Astuti Ni Kadek Ari 
Published: December 2023

Stunting is a condition of failure to thrive in children under five which is characterized by a lack of height compared to other children of their age. Although there was a decrease in the proportion of stunting from 37.2% in 2013 to 30.8% in 2018, this figure is still far from the target of WHO, which is 20%. In stunted children, there is a change in the intestinal microbiota which causes disruption of nutrient absorption, leading to malnutrition. The microbiota in healthy children contains many probiotic species such as Bifidobacterium longum and Lactobacillus mucosae, while in stunted children there are many inflammogenic taxa such as the genus Desulfovibrio. the order of Campylobacterales, E. coli, Firmicutes, Proteobacteria, and Bacterioides.
Sistem Monitoring Kelembaban Tanah dengan Sensor Soil Moisture Berbasis Internet of Things
Authors: Mahfud Faisal 
Published: December 2023

Kondisi tanah daerah wajik cepat kering apabila tidak mendapat air yang cukup karena lahan yang bersifat terbuka maka diperlukan pemantauan secara berkala terhadap tanah untuk mendapat hasil panen yang maksimal. Penelitian ini bertujuan untuk membuat dan merancang sistem monitoring kelembaban tanah dengan sensor soil moisture berbasis website dan melakukan proses monitoring berdasarkan hasil sensor soil moisture. Metode yang digunakan merupakan metode rule base yaitu metode yang menggunakan rules sebagai representasi pengetahuan untuk diimplementasikan ke dalam suatu sistem yang dirancang, implementasi rule base diterapkan pada kondisi nilai sensor soil moisture untuk membaca kondisi kelembaban tanah. Hasil penelitian berupa sistem monitoring kelembaban tanah dengan pembacaan sensor soil moisture yang ditampilkan secara real time pada website dan notifikasi telegram dengan akurasi hasil yang bisa digunakan untuk memantau kondisi kelembaban tanah pada lahan untuk memperoleh hasil panen yang maksimal.
ddPCR provides a sensitive test compared with GeneXpert MTB/RIF and mNGS for suspected Mycobacterium tuberculosis infection
Authors: Zhang Dan , Yu Fei , Han Dongsheng , Chen Weizhen , Yuan Lingjun , Xie Mengxiao , Zheng Jieyuan , Wang Jingchao , Lou Bin , Zheng Shufa , Chen Yu 
Published: December 2023

IntroductionThe Metagenomics next-generation sequencing (mNGS) and GeneXpert MTB/RIF assay (Xpert) exhibited a sensitivity for tuberculosis (TB) diagnostic performance. Research that directly compared the clinical performance of ddPCR analysis, mNGS, and Xpert in mycobacterium tuberculosis complex (MTB) infection has not been conducted.MethodsThe study aimed to evaluate the diagnostic performance of ddPCR compared to mNGS and Xpert for the detection of MTB in multiple types of clinical samples. The final clinical diagnosis was used as the reference standard.ResultsOut of 236 patients with suspected active TB infection, 217 underwent synchronous testing for tuberculosis using ddPCR, Xpert, and mNGS on direct clinical samples. During follow-up, 100 out of 217 participants were diagnosed with MTB infection. Compared to the clinical final diagnosis, ddPCR produced the highest sensitivity of 99% compared with mNGS (86%) and Xpert (64%) for all active MTB cases. DiscussionTwenty-two Xpert-negative samples were positive in mNGS tests, which confirmed the clinical diagnosis results from ddPCR and clinical manifestation, radiologic findings. Thirteen mNGS-negative samples were positive in ddPCR assays, which confirmed the clinical final diagnosis.ddPCR provides a higher sensitive compared to Xpert and mNGS for MTB diagnosis, as defined by the high concordance between ddPCR assay and clinical final diagnosis.
Longitudinal outcomes of final kissing balloon inflation in coronary bifurcation lesions treated with a single stent
Authors: He Lefan , Robb John F. , Martinez-Camblor Pablo , Andrus Bruce W. , Greene Lily J. , Gongal Prajesh , Reddy Dhruthi S. , DeVries James T. 
Published: December 2023

BackgroundFinal kissing balloon inflation (FKBI) is a percutaneous coronary intervention (PCI) technique that is considered mandatory to improve outcomes in two-stent strategies, but its use in single-stent bifurcation PCI remains controversial.MethodsIn this retrospective cohort study, we identified patients with coronary bifurcation lesions treated with one stent from January 2012 to March 2021 at a single academic medical center. Incidence rates per 1,000 patient-years (IR1000) were calculated for the outcomes of all-cause mortality, myocardial infarction (MI), stent thrombosis (ST), target lesion revascularization (TLR), coronary artery bypass graft (CABG), and cardiac readmission between patients who received FKBI and those who did not over a median follow up of 2.3 years. Studied outcomes were adjusted for all baseline clinical and procedural characteristics.ResultsThis study included 893 consecutive patients of which 256 received FKBI and 637 did not. The IR1000 for MI were 51.1 and 27.6 for patients who received FKBI and patients who did not, respectively (adjusted HR = 2.44, p = 0.001). The IR1000 for death were 31.2 and 52.3 for patients who received FKBI and patients who did not, respectively (adjusted HR = 0.68, p = 0.141). The incidence rates of ST, TLR, CABG, and cardiac readmissions were similar between patients who received FKBI and those who did not.ConclusionsThese results suggest that performing FKBI in a one-stent technique was associated with higher rates of myocardial infarction, particularly in the first 6 months, and no difference in death, ST, TLR, CABG, and cardiac readmission rates.
Case Report: SCN5A mutations in three young patients with sick sinus syndrome
Authors: Liang Jiayu , Luo Suxin , Huang Bi 
Published: December 2023

BackgroundSick Sinus Syndrome (SSS) is generally regarded as a degenerative disease with aging; however, genetic mutations have been confirmed to be associated with SSS. Among them, mutations in SCN5A are common in patients with SSS. We report three young SSS patients with SCN5A mutations at different sites that have not been previously reported in Asian patients.Case presentationThe three patients were all young females who presented with symptoms of severe bradycardia and paroxysmal atrial flutter, for which two patients received ablation therapy. However, after ablation, Holter monitoring indicated a significant long cardiac arrest; therefore, the patients received pacemaker implantation. The three patients had familial SSS, and genetic testing was performed. Mutations were found in SCN5A at different sites in the three families. All three patients received pacemaker implantation, resulting in the symptoms of severe bradycardia disappearing.ConclusionSCN5A heterozygous mutations are common among patients clinically affected by SSS. Their causative role is confirmed by our data and by the co-occurrence of genetic arrhythmias among our patients. Genetic testing for SSS cannot be performed as a single gene panel because of feasible literature results, but in presence of familial and personal history of SSS in association with arrhythmias can provide clinically useful information.
The adverse effect of gestational diabetes mellitus and hypertensive disorders of pregnancy on maternal–perinatal outcomes among singleton and twin pregnancies: a retrospective cohort study (2011–2019)
Authors: Nawsherwan  , Liu Zhiyi , Le Zhang , Mubarik Sumaira , Sun Yanmei , Naeem Shafaq , Li Hui 
Published: December 2023

BackgroundGestational diabetes mellitus (GDM) and hypertensive disorders of pregnancy (HDP) are the predominant pregnancy complications among singleton and twin pregnancies worldwide. Our primary objective was to explore the adverse effect of GDM and HDP on maternal–perinatal outcomes compared with non-GDM and non-HDP in singleton and twin pregnancies. The secondary objective was to find the risk of adverse maternal–perinatal outcomes in twin pregnancies compared with singleton pregnancies complicated with GDM and HDP in Hubei, China.MethodsA tertiary hospital-based retrospective study was conducted at Wuhan University Renmin Hospital, Hubei Province, China, from 2011 to 2019. A chi-square test was used to determine the difference in adverse maternal–perinatal outcomes between singleton and twin pregnancies. A multiple binary logistic regression model and a joinpoint regression model were used to determine the association of GDM and HDP with adverse maternal–perinatal outcomes and GDM and HDP temporal trend among singleton and twin pregnancies.ResultsThe trend of HDP [average annual percentage change (AAPC) 15.1% (95% confidence interval (95%CI): 5.3, 25.7)] among singleton pregnancies and GDM [AAPC 50.4% (95%CI: 19.9, 88.7)] among twin pregnancies significantly increased from 2011 to 2019. After adjusting for confounding factors, GDM is associated with an increased risk of C-section (adjusted odds ratio (aOR), 1.5; 95%CI: 1.3, 1.6) and macrosomia (aOR, 1.3; 95%CI: 1.1, 1.6) in singleton and preterm birth (PTB) (aOR, 2.1; 95%CI: 1.2, 3.3) in twin pregnancies compared with non-GDM. HDP was associated with a higher risk of C-section, PTB, perinatal mortality, and low birth weight (LBW) in both singleton and twin pregnancies compared with the non-HDP. Compared with singleton pregnancies complicated with GDM and HDP, twin pregnancies showed higher odds of C-section [(aOR, 1.7; 95%CI: 1.1, 2.7), (aOR, 4.6; 95%CI: 2.5, 8.7), respectively], PTB [(aOR, 22.9; 95%CI: 14.1, 37.3), (aOR, 8.1; 95%CI: 5.3, 12.3), respectively], LBW [(aOR, 12.1; 95%CI: 8.2, 18.1), (aOR, 5.1; 95%CI: 3.6, 7.4), respectively], and low Apgar score [(aOR, 8.2; 95%CI: 4.4, 15.1), (aOR, 3.8; 95%CI: 2.4, 5.8), respectively] complicated with GDM and HDP.ConclusionIn conclusion, GDM showed an increased risk of a few adverse maternal–perinatal outcomes and HDP is associated with a higher risk of several adverse maternal–perinatal outcomes in singleton and twin pregnancies compared to non-GDM and non-HDP. Moreover, twin pregnancies complicated with GDM and HDP showed higher odds of adverse maternal–neonatal outcomes compared with singleton pregnancies complicated with GDM and HDP.
Case Report: Extraocular muscles paralysis associated with GAD65 antibody: a case series study
Authors: Zhang Heyu , Yue Jiajia , Lian Chun , Long Youming , He Dan 
Published: December 2023

ObjectiveTo explore the clinical manifestations of glutamic acid decarboxylase 65 (GAD65) antibody-positive patients with extraocular symptoms and the possible mechanism.MethodAssays for the presence of GAD65 antibodies were performed on patients’ serum and cerebral spinal fluid (CSF). The brain and ocular structures involved in eye movement were assessed via magnetic resonance imaging (MRI). Tests such as electromyography (EMG), particularly repetitive nerve stimulation (RNS), and neostigmine tests were utilized for differential diagnosis. Additionally, the interaction of GAD65 antibodies with muscle tissue was confirmed using immunofluorescence techniques.ResultEach patient exhibited symptoms akin to extraocular myasthenia gravis (MG), with two individuals reporting diplopia and two experiencing ptosis. GAD65 antibodies were detected in either the serum or CSF, which were shown to bind with monkey cerebellum slides and mouse muscle slides. Neuroimaging of the brain and extraocular muscles via MRI showed no abnormalities, and all patients tested negative for the neostigmine test, RNS via EMG, and the presence of MG antibodies. However, thyroid-related antibodies were found to be abnormal in four of the patients.ConclusionOur results showed that GAD65 antibodies are not only associated with encephalitis, cerebellum ataxia or stiff-person syndrome caused by the decrease of GABAergic transmission but also diplopia and ptosis. Therefore, we should pay more attention to extraocular muscle paralysis patients without pathogenic antibodies directed against the components of neuromuscular junctions.
Efficacy, safety, and survival of neoadjuvant immunochemotherapy in operable non-small cell lung cancer: a systematic review and meta-analysis
Authors: Zheng Yue , Feng Baijie , Chen Jingyao , You Liting 
Published: December 2023

BackgroundNeoadjuvant immunochemotherapy may benefit patients with non-small cell lung cancer (NSCLC), but its impact requires further investigation.MethodsA meta-analysis was conducted. PubMed, Embase, Web of Science, and the Cochrane Library were searched. The study was registered in PROSPERO (registration no. CRD42022360893).Results60 studies of 3,632 patients were included. Comparing with neoadjuvant chemotherapy, neoadjuvant immunochemotherapy showed higher pCR (RR: 4.71, 95% CI: 3.69, 6.02), MPR (RR, 3.20, 95% CI: 2.75, 3.74), and ORR (RR, 1.46, 95% CI: 1.21, 1.77), fewer surgical complications (RR: 0.67, 95%CI: 0.48, 0.94), higher R0 resection rate (RR: 1.06, 95%CI: 1.03, 1.10, I2 = 52%), and longer 1-year and 2-year OS, without affecting TRAEs. For neoadjuvant immunochemotherapy in NSCLC, the pooled pCR rate was 0.35 (95% CI: 0.31, 0.39), MPR was 0.59 (95% CI: 0.54, 0.63), and ORR was 0.71 (95% CI: 0.66, 0.76). The pooled incidence of all grade TRAEs was 0.70 (95% CI: 0.60, 0.81), and that of &gt;= grade 3 TRAEs was 0.24 (95% CI: 0.16, 0.32). The surgical complications rate was 0.13 (95% CI: 0.07, 0.18) and R0 resection rate was 0.98 (95% CI: 0.96, 0.99). The pooled 1-year OS was 0.97 (95%CI: 0.96, 0.99), and 2-year OS was 0.89 (95%CI: 0.83, 0.94). Patients with squamous cell carcinoma, stage III or higher PD-L1 performed better. Notably, no significant differences were observed in pCR, MPR, and ORR between 2 or more treatment cycles. Pembrolizumab-, or toripalimab-based neoadjuvant immunochemotherapy demonstrated superior efficacy and tolerable toxicity.ConclusionAccording to our analysis, reliable efficacy, safety, and survival of neoadjuvant immunochemotherapy for operable NSCLC were demonstrated.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022360893, identifier CRD42022360893.
De novo identification of bacterial antigens of a clinical isolate by combining use of proteosurfaceomics, secretomics, and BacScan technologies
Authors: Yang Jinyue , Zhang Xueting , Dong Junhua , Zhang Qian , Sun Erchao , Chen Cen , Miao Zhuangxia , Zheng Yifei , Zhang Nan , Tao Pan 
Published: December 2023

BackgroundEmerging infectious diseases pose a significant threat to both human and animal populations. Rapid de novo identification of protective antigens from a clinical isolate and development of an antigen-matched vaccine is a golden strategy to prevent the spread of emerging novel pathogens.MethodsHere, we focused on Actinobacillus pleuropneumoniae, which poses a serious threat to the pig industry, and developed a general workflow by integrating proteosurfaceomics, secretomics, and BacScan technologies for the rapid de novo identification of bacterial protective proteins from a clinical isolate.ResultsAs a proof of concept, we identified 3 novel protective proteins of A. pleuropneumoniae. Using the protective protein HBS1_14 and toxin proteins, we have developed a promising multivalent subunit vaccine against A. pleuropneumoniae.DiscussionWe believe that our strategy can be applied to any bacterial pathogen and has the potential to significantly accelerate the development of antigen-matched vaccines to prevent the spread of an emerging novel bacterial pathogen.
Damaging effects of UVA, blue light, and infrared radiation: in vitro assessment on a reconstructed full-thickness human skin
Authors: Montero Paula , Roger Inés , Milara Javier , Cortijo Julio 
Published: December 2023

IntroductionExposure to solar radiation can cause a range of skin damage, including sunburn, erythema, skin carcinogenesis, the release of reactive oxygen species (ROS), inflammation, DNA damage, and photoaging. Other wavelengths beyond UVB, such as UVA, blue light, and infrared radiation, can also contribute to the harmful effects of solar radiation. Reconstructed full-thickness human skin has the potential to serve as effective predictive in vitro tools for evaluating the effects of solar radiation on the skin. The aim of this work was to evaluate the damaging effects of UVA, blue light, and infrared radiation in a full-thickness skin model in terms of viability, inflammation, photoaging, tissue damage, photocarcinogenesis.MethodsFull thickness skin models were purchased from Henkel (Phenion FT; Düsseldorf, Germany), and irradiated with increasing doses of UVA, blue light, or infrared radiation. Different endpoints were analyzed on the tissues: Hematoxylin-eosin staining, inflammation mediators, photoaging-related dermal markers and oxidative stress marker GPX1, evaluated by real-time quantitative PCR, as well as photocarcinogenesis markers by Western Blot.Results and DiscussionThe results showed differential responses in cytokine release for each light source. In terms of photoaging biomarkers, collagen, metalloproteinases 1 and 9, elastin, and decorin were modulated by UVA and blue light exposure, while not all these markers were affected by infrared radiation. Furthermore, exposure to UVA and blue light induced loss of fibroblasts and modulation of the photocarcinogenesis markers p53 and p21. In conclusion, the presented results suggest that the various wavelengths of solar light have distinct and differential damaging effects on the skin. Understanding the differential effects of UVA, blue light, and infrared radiation can serve as a valuable tool to investigate the efficacy of photoprotective agents in full thickness skin models.