bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendekatan Design of Experiment (DOE) untuk Menyelidiki Dampak Jenis Kelamin, IMT, dan Usia Terhadap Sit Up
Authors: Uscha Cindy Malinda , Arini Ratih Windu , Munikhah I Anna Tul 
Published: November 2023

Kesehatan merupakan hal yang esensial dalam kehidupan. Olahraga memainkan peran penting dalam mencegah penyakit kesehatan dan stroke. Manfaat kesehatan dari melakukan olahraga teratur, seperti sit-up, telah terbukti dalam banyak hasil kebugaran fisik. Penelitian ini bertujuan untuk menguji pengaruh beberapa faktor seperti Umur, Jenis Kelamin, dan Berat Badan terhadap tingkat kebugaran jasmani seseorang yang diukur dengan jumlah sit up. Pendekatan Design of Experiment (DoE) dengan implementasi metode 2k factorial design digunakan untuk menyelidiki penyebab perubahan respon dengan memvariasikan variabel variabel yang mempengaruhi. Hasil penelitian menunjukkan bahwa ada pengaruh faktor (Umur, Jenis Kelamin, dan Berat Badan) dan interaksi (Usia-Jenis, Umur-Berat, dan Jenis Kelamin-Berat) terhadap kemampuan jumlah sit up. Sementara itu, faktor Usia dianggap paling erat kaitannya dengan hasil tes. Secara umum, responden muda dapat melakukan beberapa sit-up lebih baik dibandingkan responden lainnya.

Kata kunci: berat badan, design of experiment, jenis kelamin, umur, 2k factorial design.
Exonuclease-III assisted the target recycling coupling with hybridization chain reaction for sensitive mecA gene analysis by using PGM
Authors: Du Yan , Xiu Ning 
Published: November 2023

Abstract
In the field of neonatal infections nursing, methicillin-resistant Staphylococcus aureus (MRSA) is a major bacterial pathogen. Here, we present a portable biosensor for MRSA detection that is both highly sensitive and portable, owing to its implementation on the personal glucose meter (PGM) platform. The H probe was fixed on the magnetic bead for mecA gene analysis. A blunt 3’ terminus appeared in the MBs-H probe when the mecA gene was present. Exonuclease-III (Exo-III) recognized the blunt terminus and cleaved it, freeing the mecA gene and so facilitating target recycling. In the meantime, the remaining H probe-initiated hybridization chain reaction (HCR), which led to the desired signal amplification. Portable quantitative detection of mecA gene is possible because PGM can read the quantity of invertase tagged on HCR product. After optimizing several experimental parameters, such as the concentration of Exo-III and incubation time, the constructed sensor is extremely sensitive, with a detection limit of 2 CFU/mL. The results from this sensitive PGM-based sensor are in agreement with those obtained from plate counting methods, suggesting that it can be used to accurately assess the MRSA content in artificial clinical samples. In addition, the PGM sensor can significantly cut down on time spent compared to plate counting techniques. The manufactured sensor provides a promising option for accurate identification of pathogenic bacteria.
Photoacclimation of Corals in the Turbid Waters of the Northern Malacca Straits, Malaysia
Authors: MUBIN Nur Ain Amani ABDUL , SALLEH Sazlina , KAMPHOL Nadthikphorn , MOHAMMAD Mahadi , JONIK Michelle Glory G , CHEAH Wee , HOSSAIN Md Solaiman 
Published: November 2023

Abstract

Reefs in the Northern Straits of Malacca are exposed to low-light conditions mainly due to sedimentation. Corals can be found in the urban reefs of Pulau Kendi (PK) and Pulau Songsong (PS). Rapid Light Curves (RLCs) measurements were performed in situ using a Diving Pulse Amplitude Modulated (PAM) fluorometer to investigate the photoacclimation of hard corals in the turbid waters of non-protected reefs in PK and PS. Despite the high turbidity at both sites, high derived maximum photochemical efficiency values (Fq′/Fm′(max)) were observed in all coral species at PK (0.77 to 0.80) and PS (0.70 to 0.76). Corals in PS had lower photosynthetic efficiency (α) ranged from 0.08 to 0.09 and maximum relative electron transport rate (rETRmax) ranged from 62.88 ± 6.16 to 136.11 ± 5.70 compared to those in PK (α range: 0.26–0.32; rETRmax range: 102.93 ± 138.41–138.4 ± 5.1). These differences were associated with low non-photochemical quenching (NPQ) (0.08 ± 0.00–0.23 ± 0.00) and symbiont density (0.05 ± 0.00 cells cm2 x 106 – 0.23 ± 0.06 cells cm2 x 106) in PS. The results indicate that coral physiology varies significantly among sites and species, and corals can acclimate to changes in the environment. However, the extent of acclimation depends on light availability in their habitat, which may play a critical role in their survival and persistence in harsh environments.
Possible correlation of selected immune genes of tiger shrimp, Penaeus monodon with respect to white spot syndrome virus (WSSV) infection
Authors: Saravanan P. , Praveena P. Ezhil , Bhuvaneswari T. , Otta Subhendu Kumar 
Published: November 2023

Abstract
White spot syndrome virus (WSSV) of shrimp is a highly virulent pathogen, which can bring mass mortality and thereby severe economic loss to the industry. Though shrimp has a less developed immune system, several gene components have been discovered which play certain and specific roles. Defense response of shrimp with respect to particular pathogen such as WSSV needs to be understood. The current study investigated the physiological changes and gene expression pattern with respect to silencing of Relish, IAP or Rab7 in Penaeus monodon by gene specific dsRNA followed by flow cytometry analysis after WSSV infection. RNAi has been used as a powerful tool to analyze the gene function in worms, shrimps, insects and plants. Knockdown of specific defense genes by dsRNA treatment and functional pattern of other intact genes were observed through expression analysis and flow cytometry. Penaeus monodon signal transducer and activator of transcription (PmSTAT) gene expression was found to be up regulated in case of dsRNA knocked down of either Penaeus monodon Relish transcription factor (PmRelish), inhibitor of apoptosis (IAP) explaining important role of STAT in immunity. Crustin expression was significantly down regulated in dsIAP and dsRelish injected animal. PmRelish and Crustin expression was up regulated in Rab7 silenced P. monodon. Necrosis and early apoptosis were high in PmRelish silenced P. monodon suggesting possible role of PmRelish in apoptosis pathway. Similarly, individual dsRNA silenced shrimp cells showed distinct phenotypic changes. The study indicates distinct inter-relationship between different defense genes while acting synergistically to provide overall protection against shrimp WSSV.
Weather Variability and Rheumatoid Arthritis: Uncovering Associations with Atmospheric Pressure and Temperature Changes
Authors: Lin Jing-Chi , Lin Chun-Yen , Wu Chien-sheng , Lien Frank , Lin Ko-ming , Chang Ting-Hui , Lin Kuan-Yu , Yang Hui Ching 
Published: November 2023

Abstract
Objectives
The aim of this study was to investigate the potential impact of weather on rheumatoid arthritis (RA) disease activity using registry data from the Taiwan Rheumatology Association Clinical Electronic Registry (TRACER), a mobile application used to record disease activity and medication in selected centers.
Methods
A total of 567 patients with RA were included in this study, with data on disease activity score, 28 joint-count erythrocyte sedimentation rate (DAS28-ESR), and medication (including disease modifying anti-rheumatic drugs (DMARDs) and biological DMARDs (bDMARDs) recorded in TRACER. Weather data were obtained from the observation data inquiring system of the Central Weather Bureau, Taiwan. GEE statistical models were used to analyze the association between patient's DAS28-ESR, medication and weather data.
Results
Of the 567 RA patients included in the study population, 75% were female, and the mean age at diagnosis was 52 years (range 15–84 years). The mean DAS28-ESR was 3.8 (range 0.2-8.0) in bDMARDs users and 3.6 (range 0.2–7.8) in DMARDs users. The results revealed that atmospheric pressure and temperature changes, rather than sunshine hours and relative humidity, were significantly associated with RA disease activity (p < 0.05).
Conclusion
This study demonstrated the value of TRACER as a tool for recording valuable data on daily practice, which can be exported for statistical analysis. Our findings suggest that weather changes, particularly atmospheric pressure and temperature changes, are associated with RA disease activity, especially in those patients with positive rheumatoid factors (RF).
Techno-economical evaluation of three methods for stripping gas recovery in gas dehydration by unit of Khark petrochemical complex
Authors: Abdehvand Arman Mohammadi , Haghi Mahdi , Hosseinirad Ebrahim 
Published: November 2023

Abstract

The presence of water vapor in natural gas poses several issues including corrosion, blockages, and reduced pipeline capacity. The gas dehydration is a crucial process to reduce the water content and mitigate these problems. In this paper, glycol dehydration unit of Khark Petrochemical Company (KPC) has been simulated using Aspen Hysys v11.0 process simulator. At first, the simulation results has been validated with the design data and affecting parameters on the dehydration unit performance were identified through sensitivity analysis and their effects on the produced dry gas was investigated. The results showed that increasing the solvent circulation rate, stripping gas flow rate and reboiler temperature leads to more water removal rate. Secondly, the several options are suggested for stripping gas recovery and capital and operating expenditure for each option has been estimated and techno-economical evaluation of stripping gas recovery was carried out. Our techno-economic analysis reveals that about 0.7 MMSCFD stripping gas can be recovered from regeneration towers by increasing the operating pressure of regenerator and separating water from stripping gas. In the optimum option (sending the stripping gas to low pressure feed gas compressor), this project requires about 80,000 $ capital cost and has 1.7 MM$ annual revenue.
Measuring Personal Emergency Preparedness: Validation of the Emergency Preparedness Checklist Across Three Samples
Authors: Talisman Nicholas W. , Rohrbeck Cynthia , Moore Phillip J. , Marceron Jennifer E. , Burns Katherine M 
Published: November 2023

Abstract

Large-scale emergencies such as natural and human-made disasters (e.g., hurricanes, terrorist attacks) have profound, deleterious effects on human well-being, including loss of life, physical injury, psychological trauma, and financial devastation. Personal or household emergency preparedness can mitigate the impact of these disasters. Effective and psychometrically sound measures of preparedness are critical to identifying individual differences in these behaviors and testing theories of emergency preparedness. However, there are few personal preparedness measures appropriate for all disaster types, and fewer still with evidence of reliability and validity. This paper describes the development and psychometric validation of a new Emergency Preparedness Checklist (EPC), using three separate samples -- university undergraduates, adults living in the DC Metro Area, and a nationwide sample of adults with physical disabilities – to demonstrate its potential generalizability. Across samples, the EPC had high levels of internal consistency and demonstrated concurrent validity. In addition, research with the EPC has shown that it is related to other constructs (e.g., perception of disaster threat, self-efficacy for emergency preparedness) as expected based on disaster theories. These findings support the EPC’s utility in research on disasters and preparedness.
Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins
Authors: Garduño Roberto González , Vázquez María Sol Arias , Monteiro Cristiana Cazapal , Sánchez-Andrade Rita , Paz-Silva Adolfo 
Published: November 2023

Abstract

Cyathostomins are gastrointestinal nematodes (GIN) that affect equines in many parts of the world. Due to anthelmintic resistance (AR), it is necessary to search for control alternatives. The objective of the study was to carry out a screening of 26 essential oils (EOs) from plants to determine in vitro their action on cyathostomins. Essential oils were obtained by hydro-distillation from leaves, flowers, fruits and seeds of the selected species and used against the eggs of cyathostomins by means of the tests of egg hatching and larval motility inhibition. For each EO, different concentrations were prepared (250, 125, 62.5, 31.3, 15.6, 7.8, 3.9, 2.0, 1.0, 0.5 and 0.24 mg/mL). Oxfendazole at 2.5% mixed with closantel 5% was used as positive control, and PBS served as negative control. Cyathostomin eggs were obtained from feces of infected horses and infective larvae were recovered after de coproculture. The in vitro test was carried out 36 hours after the challenge con the EOs. The highest production of EOs was obtained from leaves of bay, eucalyptus, mallow and basil, as well as from seeds of black pepper, coriander, cinnamon, anise, and peel of orange and cloves, cumin and Lawson’s cypress. The oils extracted from laurel leaves, ground cinnamon, anise, cumin, and coriander seeds had a very high effect on cyathostomins eggs up to doses lower than 3.9 mg/mL. Therefore, in addition to the uses in other species such as fungi and bacteria, the use of EOs in the control of nematode eggs gives greater importance as alternative control.
It is the habit not the handle that affects tooth brushing. Results of a randomised counterbalanced cross over study
Authors: Deinzer Renate , Eidenhardt Zdenka , Sohrabi Keywan , Stenger Manuel , Kraft Dominik , Sick Bernhard , Götz-Hahn Franz , Bottenbruch Carlotta , Berneburg Nils , Weik Ulrike 
Published: November 2023

Abstract
Objective:
To assess the effect of the toothbrush handle on video-observed toothbrushing behaviour and toothbrushing effectiveness
Methods:
This is a randomized counterbalanced cross-over study. N = 50 university students brushed their teeth at two occasions, one week apart, using either a commercial ergonomically designed manual toothbrush (MT) or Brushalyze V1 (BV1), a manual toothbrush with a thick cylindrical handle without any specific ergonomic features. Brushing behaviour was video-analysed. Plaque was assessed at the second occasion immediately after brushing. Participants directly compared the two brushes regarding their handling and compared them to the brushed they used at home.
Results:
The study participants found the BV1 significantly more cumbersome than the M1 or their brush at home. (p < 0.05). However, correlation analyses revealed a strong consistency of brushing behavior with the two brushes (0.71 < r < 0.91). Means differed only slightly (all d < 0.36). These differences became statistically significant only for the brushing time at inner surfaces (d = 0.31 p = 0.03) and horizontal movements at inner surfaces (d = 0.35, p = 0.02). Plaque levels at the gingival margins did not differ while slightly more plaque persisted at the more coronal aspects of the crown after brushing with BV1 (d = 0.592; p 0.042)
Discussion:
The results of the study indicate that the brushing handle does not play a major role in brushing behavior or brushing effectiveness.
Zhen-Wu-Tang decoction ameliorates lupus nephritis by diminishing renal tissue-resident memory CD8+ T cells via suppressing IL15/STAT3 pathway
Authors: Liang Chunling , Wei Yuyan , Luo Yini , Qin Fenglian , Chen Yuchao , Liu Huazhen , Qiu Feifei , wu Junbiao , Liu Yuntao , Dai Zhenhua 
Published: November 2023

Abstract
Background
Zhen-Wu-Tang (ZWT), a traditional herbal formula, has been recommended for treating lupus nephritis (LN) in clinic, but the mechanisms underlying its effects remain unclear. Here we aimed to define the immunological mechanisms underlying its effects on LN.
Methods
In this study, murine LN was induced by a single injection of pristane, while in vitro TRM cells differentiated in the presence of IL-15/TGF-β. The 24h-urine protein, serum creatinine (Scr), serum anti-dsDNA Ab, H&E stain and renal depositions of IgG/complement 3 (C3) were measured to evaluate renal injury and function. Immunoassays, including immunohistochemistry, immunofluorescence, flow cytometry and ELISA, were performed to quantify TRM cells, macrophages polarization and STAT activation both in vivo and in vitro.
Results
We found that ZWT or mycophenolate mofetil treatment significantly ameliorated kidney injury in LN mice by decreasing 24-h urine protein, Scr and anti-dsDNA Ab. ZWT also improved renal histology and reduced the deposition of IgG and C3. In addition, ZWT downregulated renal desmin expression. Moreover, it significantly reduced the number of renal CD8+ TRM in LN mice while decreasing expression of TNF-α and IFN-γ, but not ki67, in renal CD8+ TRM cells. Consistent with in vivo results, ZWT-containing serum inhibited TRM cell differentiation induced by IL-15/TGF-β ex vivo, but it had no effect on ki67 expression. Importantly, ZWT reduced the number of total F4/80+CD11b+ and CD86+, but not CD206+, macrophages in the kidney of LN mice. Interestingly, ZWT decreased IL-15 protein expression in the kidney tissue of LN mice. Further, it mainly suppressed phosphorylation of STAT3 in CD8+ TRM cells in vitro.
Conclusions
We have provided the first evidence that ZWT decoction can be used to improve LN outcome by reducing CD8+ TRM cells via inhibition of IL-15/STAT3 signaling.
Exploring the microbiota to better understand pancreatic cancer physiology
Authors: Chen Anli , Zhao Feng , Wu Xiaotian , Deng Xiangyu , Yang Jiali , Xue Jianjiang 
Published: November 2023

Abstract
Pancreatic cancer is a leading cause of cancer death worldwide and its global burden of disease is increasing year by year. This study explores the microbiota of pancreatic cancer patients with the aim of better understanding the physiology of pancreatic cancer. A total of 14 patients diagnosed with pancreatic cancer and 14 patients with chronic pancreatitis were included, and serum samples and lesion tissues of both groups were obtained under the premise of informed consent. Fresh stool samples were collected from 29 pancreatic cancer patients, and gut microbial data from normal control populations were obtained from the SRA database (SRA data number: SRP150089).16S rRNA sequencing was used to analyze the microbial profile and diversity of feces and lesion tissue samples. The results showed that the expression levels of tumor markers CA242 and CA199 were lower in the chronic pancreatitis than in the pancreatic cancer (P < 0.05); the pancreatic cancer patient group showed increased gut microbial α diversity and decreased number of beneficial bacteria compared with the healthy control group; meanwhile, the pancreatic cancer group showed increased tumor microbial α diversity and altered species composition compared with the chronic pancreatitis; In addition, the expression level of CA242 was positively correlated with Acinetobacter (P < 0.05).The microbiota of patients with pancreatic cancer is specifically altered, and Acinetobacter may be a marker for early screening for pancreatic cancer.
Growing of perfect single-crystal epitaxial films of (Si 2 ) 1-x (GaN) x solid solutions on Si (111) substrates from the liquid phase
Authors: Saparov Dadajon , Saidov A. S. , Usmonov Sh. N. , Ishniyazov T. T. , Kalanov M. U. , Akhmedov A. M. 
Published: November 2023

Abstract
The technological capabilities of the method of liquid-phase epitaxy from a limited volume of Sn solution-melt for obtaining films of substitutional solid solution (Si2)1−x(GaN)x on Si (111) substrates are shown. The grown films had a single-crystal structure with (111) orientation, n-type conductivity with a resistivity of ρ ~ 1.38 Ω∙cm, a carrier concentration of n ~ 3.4∙1016 cm− 3, and a charge carrier mobility of µ ~ 133 cm2/(V⋅sec). The relatively narrow width (FWHM = 780 arcsec) and high intensity (2⋅105 pulses/sec) of the main structural reflection (111)Si/GaN indicate a high degree of perfection of the crystal lattice of the epitaxial layer (Si2)1−x(GaN)x. The photosensitivity region of p-Si–n-(Si2)1−x(GaN)x heterostructures covers the photon energy range from 1.2 to 2.4 eV, with a maximum at 1.9 eV.
Cystatin C and sarcopenia index are associated with all-cause and cardiovascular death among adults in the United States
Authors: Wang Tianbo , Zhu Yuxin , Liu Xiaohan , Zhang Yue , Zhou Shihang , Zhang Zhen , Wu Jin , Huang Gang , Xu Junbo 
Published: November 2023

Abstract

Objectives
This study aimed to investigate the potential association between cystatin C, creatinine, and the sarcopenia index (SI) and cardiovascular and all-cause death in general population.
Methods
Data of participants from the National Health and Nutrition Examination Surveys (NHANES) from 1999 to 2004 were used and all participants were followed up regularly until December 31, 2019. Multivariable Cox analysis was used to investigate the potential association between the potential risk factors and cardiovascular and all-cause death. Restricted cubic spline regression was conducted to evaluate the potential nonlinearity between potential risk factors and death.
Results
In this study, 9894 participants with a mean age of 45.64 years were enrolled and followed up for a mean duration of 15.62 ± 4.68 years. About 50.3% were men and a total of 2681 all-cause deaths and 691 cardiovascular deaths were recorded. In multivariable Cox models, higher cystatin C level (≥ 0.877 vs. <0.659) was associated with an increased risk of cardiovascular death (HR:3.36, 95% CI: 2.06–5.46, p < 0.001) and all-cause death (HR:2.71, 95% CI: 2.17–3.38, p < 0.001). However, lower SI (< 88.41 vs. ≥125.52) was associated with a higher risk of cardiovascular death (HR:2.56, 95% CI: 1.88–3.49, p < 0.001) and all-cause death (HR:2.45, 95% CI: 2.07–2.90, p < 0.001). Additionally, a J-shaped curve association was found between cardiovascular and all-cause death and creatinine.
Conclusions
Cystatin C and SI are associated with cardiovascular and all-cause death in general population in the United States. There is a J-shaped curve association between creatinine and cardiovascular and all-cause death among US adults.
Cigarette smoking and cardiovascular disease incidence and all-cause mortality: the modifying role of diet quality
Authors: Norouzzadeh Mostafa , Teymoori Farshad , Farhadnejad Hossein , Moslehi Nazanin , Mirmiran Parvin , Rahideh Seyedeh Tayebeh , Azizi Fereidoun 
Published: November 2023

Abstract
Background
This study examines the potential long-term interaction between smoking and diet as modifiable risk factors concerning cardiovascular disease (CVD) incidence and all-cause mortality in the Tehran Lipid and Glucose Study framework.
Method
The study followed 955 smokers from the third and fourth examinations and collected dietary data using a food frequency questionnaire to calculate three different diet quality indices (DQIs): DQI-international, DQI-revised, and Mediterranean-DQI. Current smokers were classified according to smoking intensity and pack-year index. Cox proportional hazards regression models were used to determine the HR (95% CI) of the impact of DQIs, smoking status, and their interaction on CVD incidence and all-cause mortality.
Result
Over a follow-up period of almost 8 years, 94 cases of CVD (9.8%) and 40 cases of mortality (4.2%) were documented. Although no significant association was found between adherence to the DQI-I and DQI-R indices and the studied outcomes, the study found that lower diet quality based on the Med-DQI was associated with a higher risk of mortality among current smokers (HR: 3.45; 95%CI:1.12, 10.57). Light smokers with higher scores on the DQI-I and DQI-R, compared to heavy smokers with poor diet quality, had a lower risk of CVD incidence (HR: 0.35; 95%CI: 0.35, 0.83) and mortality (HR: 0.20; 95%CI:0.05, 0.77), respectively. Smoking cessation reduced mortality risk (HR: 0.35; 95%CI:0.12, 1.03), and this risk reduction was greater when accompanied by improved diet quality (HR: 0.10; 95%CI:0.02, 0.45).
Conclusion
Improving diet quality and quitting smoking can lower the risk of CVD and mortality for smokers.