bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Perfusable Multi‐Hydrogel Vasculature On‐Chip Engineered by 2‐Photon 3D Printing and Scaffold Molding to Improve Microfabrication Fidelity in Hydrogels
Authors: Cantoni Federico , Barbe Laurent , Pohlit Hannah , Tenje Maria 
Published: January 2024

AbstractEngineering vasculature networks in physiologically relevant hydrogels represents a challenge in terms of both fabrication, due to the cell–bioink interactions, as well as the subsequent hydrogel‐device interfacing. Here, a new cell‐friendly fabrication strategy is presented to realize perfusable multi‐hydrogel vasculature models supporting co‐culture integrated in a microfluidic chip. The system comprises two different hydrogels to specifically support the growth and proliferation of two different cell types selected for the vessel model. First, the channels are printed in a gelatin‐based ink by two‐photon polymerization (2PP) inside the microfluidic device. Then, a human lung fibroblast‐laden fibrin hydrogel is injected to surround the printed network. Finally, human endothelial cells are seeded inside the printed channels. The printing parameters and fibrin composition are optimized to reduce hydrogel swelling and ensure a stable model that can be perfused with cell media. Fabricating the hydrogel structure in two steps ensures that no cells are exposed to cytotoxic fabrication processes, while still obtaining high fidelity printing. In this work, the possibility to guide the endothelial cell invasion through the 3D printed scaffold and perfusion of the co‐culture model for 10 days is successfully demonstrated on a custom‐made perfusion system.
Developing Graphene‐based Conductive Textiles Using Different Coating Methods
Authors: Abdi Babak , Tarhini Ali , Baniasadi Hossein , Tehrani‐Bagha Ali R. 
Published: January 2024

AbstractIn this research, a series of graphene‐based conductive textiles is developed by three different coating methods, including dip‐coating (D), airbrushing (A), and filtration (F). The cellulose substrate consists of a blend of cotton and rayon fabric, and the coating formulation is based on a mixture of graphene powder as a conductive filler, polyurethane (PU) as a binder, and poly(vinylidene fluoride‐co‐hexafluoropropylene) (PVDF‐HFP) as a thermoplastic co‐binder. The thermogravimetric analysis (TGA) results are used to prove an enhancement in the thermal stability of the coated fabrics. The graphene content of the coated samples is also estimated from the char residue at 800 °C of the TGA profiles. The graphene‐based coating converts the water‐adsorbing cellulose fabric to a hydrophobic surface as the water contact angle raises from 0° to more than 107° after coating. The mechanical properties of the plain cellulose fabric enhance considerably in terms of tensile strength and tensile modulus, where the highest improvement is seen in the Dip‐coating method, with an 89% increase in tensile strength compared to cellulose fabric. The graphene‐based coating developed in this work enhances the physical, thermal, mechanical, and conductivity properties of the plain cellulose substrate. The resulting coated fabrics can be potentially used in wearable smart electronic textiles.
A Low‐Cost, User‐Friendly Rheo‐Optical Compression Assay to Measure Mechanical Properties of Cell Spheroids in Standard Cell Culture Plates
Authors: Ferraro Rosalia , Caserta Sergio , Guido Stefano 
Published: January 2024

AbstractThe mechanical characterization of cell spheroids, one of the most widely used 3D biology models in vitro, is a hotspot of current research on the role played by the mechanical response of cells and tissues. The techniques proposed so far in the literature, while providing important scientific insights, require specialized equipment and technical skills that are not usually available in cell biology facilities. Here, an innovative rheo‐optical compression assay is presented: it is based on microscopy glass coverslips as the load is applied to cell spheroids in standard cell culture plates and on image acquisition with an optical microscope or even a smartphone equipped with adequate magnification lenses. Mechanical properties can be simply obtained by correlating the applied load to the deformation of cell spheroids measured by image analysis. The low‐cost, user‐friendly features of the proposed technique can boost mechanobiology research making it easily affordable to any biomedical lab equipped with cell culture facilities.
LaAlO3/SrTiO3 Heterointerface: 20 Years and Beyond
Authors: Chen Shunfeng , Ning Yuanjie , Tang Chi Sin , Dai Liang , Zeng Shengwei , Han Kun , Zhou Jun , Yang Ming , Guo Yanqun , Cai Chuanbing , Ariando Ariando , Wee Andrew T. S. , Yin Xinmao 
Published: January 2024

AbstractThis year marks the 20th anniversary of the discovery of LaAlO3/SrTiO3 (LAO/STO) oxide heterointerfaces. Since their discovery, transition metal oxide (TMO) interfaces have emerged as a fascinating and fast‐growing area of research, offering a variety of unique and exotic physical properties which has provided a strong impetus for the rapid advances and actualization of oxide electronics. This review revisits the fundamental mechanisms accounting for the two‐dimensional (2D) conducting interfaces, and how new models proposed to better account for the unique interfacial effects. Recent breakthroughs in the theoretical and experimental domains of oxide interfaces are also discussed including the detection and investigation of 2D quasiparticle. Moving beyond the well‐known LAO/STO interface, this review delves into other systems where unconventional interfacial superconductivity, interfacial magnetism, and spin polarization are dealt with in greater detail. In terms of device applications, this review proceeds with a treatment on the recent developments in domains including field effect transistors and freestanding heterostructure membranes. By emphasizing the opportunities and challenges of integrating oxide interfaces with existing technologies, the review will end off with an outlook projecting the progress and the trajectory of this research domain in the years to come.
Extrusion bioprinting of elastin‐containing bioactive double‐network tough hydrogels for complex elastic tissue regeneration
Authors: Wang Di , Zeng Jinshi , Zhu Hailin , Liu Siyu , Jia Litao , Liu Wenshuai , Wang Qian , Wang Senmao , Liu Wei , Zhou Jiayu , Chen Huimin , Liu Xia , Jiang Haiyue 
Published: January 2024

AbstractDespite recent advances in extrusion bioprinting of cell‐laden hydrogels, using naturally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge. Here, we address this challenge with precisely designed biological tough hydrogel bioinks featuring a double‐network structure. The tough hydrogels consisted of energy‐dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid (o‐nitrobenzyl‐grafted hyaluronic acid) and elastin through Schiff's base reaction, and free‐radically polymerized gelatin methacryloyl. The incorporation of elastin further improved the elasticity, stretchability (∼170% strain), and toughness (∼45 kJ m−3) of the hydrogels due to the random coiling structure. We used this novel class of hydrogel bioinks to bioprint several complex elastic tissues with good shape retention. Furthermore, in vitro and in vivo experiments also demonstrated that the existence of elastin in the biocompatible bioinks facilitated improved cell behaviors and biological functions of bioprinted tissues, such as cell spreading and phenotype maintenance as well as tissue regeneration. The results confirmed the potential of the elastin‐containing tough hydrogel bioinks for bioprinting of 3D complex elastic tissues with biological functionalities, which may find widespread applications in elastic tissue regeneration.
Unilateral sacral erector spinae plane block for hip fracture surgery
Authors: Marrone F. , Paventi S. , Tomei M. , Failli S. , Crecco S. , Pullano C. 
Published: January 2024

SummaryThe provision of anaesthesia for hip fracture surgery in elderly and frail patients can be challenging, with potentially significant risks associated with both general and neuraxial techniques. Here, we report the use of a sacral erector spinae plane block as an alternative to conventional anaesthetic approaches for a frail 89‐year‐old woman with significant cardiovascular and respiratory comorbidity who underwent intramedullary nailing for a proximal femoral fracture. A unilateral injection of local anaesthetic at the intermediate crest of the second sacral vertebra resulted in bilateral sensory block of the T12 to S2 dermatomes. The technique did not result in hypotension or motor block of the limbs, and the surgery was completed uneventfully. Sacral erector spinae plane block warrants further investigation as an alternative to spinal and general anaesthesia for hip and lower limb surgery.
Investigation of chemical properties and bioactive compounds of oils from different pumpkin seeds (Cucurbita pepo L.) during maturation
Authors: Dülger Gizem Çağla , Geçgel Ümit 
Published: January 2024

AbstractIn this study, seed and oil yields, protein, and moisture ratios of seeds of four different types of pumpkin seed varieties, namely Palancı population, VD1sn8, and VD1sn6 hybrid varieties and commercial variety grown in Edirne conditions in 2014 and 2015. Also, this study aimed to determine the change of fatty acid, tocopherol, and sterol composition of mentioned pumpkin seed varieties in three different periods from seed formation to final harvest time. During the ripening period, it was obtained that the oil yield increased, the moisture content of pumpkin seeds decreased. In the last harvest period, the oil yield of pumpkin seed varieties was determined to be between 37.21% and 42.07%. Protein ratios of all pumpkin seed species were found to be very close to each other (37.94%–39.28%) and statistically similar (p > 0.05). In 2014 and 2015, the dominant fatty acids for all pumpkin seed varieties are 18:1 (39.49%–46.95%) and 18:2 (32.57%–39.26%). Except for these fatty acids, 16:0 varies between 10.65% and 13.60% in all varieties; 18:0 varies at a ratio of 5.70%–6.38%. It is seen that the dominant tocopherol isomer is γ‐tocopherol for all pumpkin seed species in all harvest periods. In the last harvest period in 2014 and 2015, the amounts of γ‐tocopherol constitute 99.98%–84.95% and 86.91%–89.86% of the total tocopherol, respectively. It was observed that the tocopherol composition changed during the ripening period in all pumpkin seed species (p < 0.05). In general, the amount of sterols decreased during the ripening period for all cultivars in 2014 and 2015. In order from the highest to the least, β‐sitosterol, 5,24‐stigmastadienol, campesterol, Δ‐5 avenasterol, and stigmasterol were determined as phytosterols in pumpkin seed oils. Generally, β‐sitosterol ratios in all varieties were high in the 1st harvest period, decreased slightly in the 2nd harvest period, increased again until the 3rd harvest period and reached the values in the 1st harvest period in both 2014 and 2015.
Random sulfonated poly(arylene ether sulfone) and sulfonated poly(arylene ether ketone) membranes for fuel cell applications
Authors: Ramos‐Rivera Gilberto , Suleiman David 
Published: January 2024

AbstractIn this study, sulfonated poly(arylene ether sulfone) (SPAES) and sulfonated poly(arylene ether ketone) (SPAEK) were randomly synthesized, employing a presulfonation process. This presulfonation process resulted in a more controlled and reproducible sulfonation level. The respective polymers were prepared using 2,2‐Bis(4‐hydroxyphenyl) propane at 50% molar ratio, which also provided some membrane elasticity. The resulting polymers, each had 25% of the block containing the sulfonic domains (SPAES A 25 and SPAEK A 25). Better conductive membranes were achieved for the random sulfone polymers than for the random ketone polymers, with values, respectively, of 0.24 and 0.07 S cm−1 at 80°C. The lower proton conductivity from the ketone‐based polymer was compensated with very low methanol permeability (0.25 × 10−6 cm2 s−1) and outstanding oxidative stability. The selectivity of both polymer membranes exceeded the reported values for the state‐of‐the‐art Nafion® 117 and other commercially available options. Both polymer membranes, with their unique combination of ionic domains, elastomeric blocks, and resulting morphology, could be viable candidates for fuel cell applications.
Earthquake impacts on a protected pinniped in New Zealand
Authors: Hall Alasdair A. , Chilvers B. Louise , Weir Jody Suzanne , Boren Laura J. 
Published: January 2024

Abstract

The impacts of natural disasters on marine mammals are poorly understood and difficult to study, which can hamper management responses following such events.
This study provides the first distribution and abundance assessment of New Zealand fur seal (NZFS: Arctocephalus forsteri) colonies around Kaikōura, New Zealand, since a 7.8 magnitude earthquake in November 2016 caused substantial changes to both the local marine and terrestrial environments, and led to the reconstruction of a major highway that runs adjacent to NZFS colonies.
Mark–recapture and direct counts in the 2022–2023 breeding season estimated pup production for NZFS breeding colonies along the Kaikōura coast. Using established multipliers, pup estimates were used to provide the first comprehensive population estimate for Kaikōura's NZFS population since the earthquake.
Three new colonies and three new subcolonies were assessed and recorded, additional to reassessments of two established colonies. Overall, Kaikōura's NZFS population has grown and spread post‐earthquake, with an upper total population estimate of between 21,560 and 28,327 animals in the 2022–2023 breeding season. Some sites, such as Lynch's Reef, appear to have benefited from earthquake‐induced coastal uplift, with pup production increasing. Contrastingly, the estimated 2,401 (±99) pups produced at Ōhau Point in 2023 is similar to pre‐earthquake estimates. This indicates that the earthquake has disrupted previously documented growth at this site. The distribution of NZFS breeding at Ōhau Point has also changed substantially since the last pre‐earthquake assessment.
From these findings, alterations to the Ōhau Point New Zealand Fur Seal Sanctuary and similar protections at other locations on the Kaikōura coast are suggested, as greater numbers of NZFSs are now accessible to human interaction and disturbance. The results demonstrate both how natural disasters and subsequent infrastructure modifications can impact coastal species and how conservation measures may need to be amended accordingly.
Adaptive fuzzy finite‐time prescribed performance control for uncertain nonlinear systems with actuator saturation and unmodeled dynamics
Authors: Kang Shijia , Liu Peter Xiaoping , Wang Huanqing 
Published: January 2024

AbstractIn this article, the tracking control problem is addressed for uncertain nonlinear systems with actuator saturation and unmeasurable unmodeled dynamics. Being different from the existing performance function control results, to constraint the output tracking error within a predefined boundary in finite time, an improved performance function, that is, finite‐time performance function, is introduced. The design difficulties of asymmetric input saturation and unmodeled dynamics are solved simultaneously for the first time by applying a smooth non‐affine function and a measurable dynamic signal. Furthermore, an adaptive fuzzy control scheme is established via command filtering, which not only guarantees the semi‐global boundedness of all the controlled system signals but also makes the output tracking error that can be restrained by the described performance bound within a finite‐time interval. At last, an effective example is supplied to validate the availability of the presented theoretical finding.
Investigating the partonic entanglement entropy at low Bjorken‐x within Gluon saturation approach
Authors: Ramos Gabriel Silveira , Machado Magno V. T. 
Published: January 2024

AbstractIn this work, the entanglement entropy is examined within the context of deep inelastic scattering in collisions. The calculation is based on a formalism where the partonic state at small‐ is maximally entangled, consisting of a large number of micro‐states occurring with equal probabilities. Analytical expressions for the number of gluons, , are considered, derived from gluon saturation models for dipole‐target amplitudes within the framework of the Quantum Chromodynamics (QCD) color dipole picture. A comparison of the entanglement entropy with thermodynamic entropy measured in and collisions at high energies is done.
A computed tomography urography‐based machine learning model for predicting preoperative pathological grade of upper urinary tract urothelial carcinoma
Authors: Zheng Yanghuang , Shi Hongjin , Fu Shi , Wang Haifeng , Wang Jincheng , Li Xin , Li Zhi , Hai Bing , Zhang Jinsong 
Published: January 2024

AbstractObjectivesDevelopment and validation of a computed tomography urography (CTU)‐based machine learning (ML) model for prediction of preoperative pathology grade of upper urinary tract urothelial carcinoma (UTUC).MethodsA total of 140 patients with UTUC who underwent CTU examination from January 2017 to August 2023 were retrospectively enrolled. Tumor lesions on the unenhanced, medullary, and excretory periods of CTU were used to extract Features, respectively. Feature selection was screened by the Pearson and Spearman correlation analysis, least absolute shrinkage and selection operator algorithm, random forest (RF), support vector machine (SVM), and eXtreme Gradient Boosting (XGBoost). The logistic regression (LR) was used to screen for independent influencing factors of clinical baseline characteristics. Machine learning models based on different feature datasets were constructed and validated using algorithms such as LR, RF, SVM, and XGBoost. By computing the selected features, a radiomics score was generated, and a diverse feature dataset was constructed. Based on the training set, 16 ML models were created, and their performance was evaluated using the validation set for metrics including sensitivity, specificity, accuracy, area under the receiver operating characteristic curve (AUC), and others.ResultsThe training set consisted of 98 patients (mean age: 64.5 ± 10.5 years; 30 males), whereas the validation set consisted of 42 patients (mean age: 65.3 ± 9.78 years; 17 males). Hydronephrosis was the best independent influence factor (p < 0.05). The RF model had the best performance in predicting high‐grade UTUC, with AUC of 0.914 (95% Confidence Interval [95%CI] 0.852–0.977) and 0.903 (95%CI 0.809–0.997) in the training set and validation set, and accuracy of 0.878 and 0.857, respectively.ConclusionsAn ML model based on the RF algorithm exhibits excellent predictive performance, offering a non‐invasive approach for predicting preoperative high‐grade UTUC.
Aerobic Catalytic Cross‐Dehydrogenative Coupling of Furans with Indoles Provides Access to Fluorophores with Large Stokes Shift
Authors: Mhaske Krishna , Gangai Shon , Fernandes Rushil , Kamble Angulimal , Chowdhury Arkaprava , Narayan Rishikesh 
Published: January 2024

AbstractSustainability in chemical processes is a crucial aspect in contemporary chemistry with sustainable catalysis as a vital parameter of the same. There has been a renewed focus on utilizing earth‐abundant metal catalysts to expand the repertoire of organic reactions. Furan is a versatile heterocycle of natural origin used for multiple applications. However, it has scarcely been used in cross‐dehydrogenative coupling. In this work, we have explored the cross‐dehydrogentive coupling of furans with indoles using commonly available, inexpensive FeCl3 ⋅ 6H2O (<0.25 $/g) as catalyst in the presence of so called ‘ultimate oxidant’ – oxygen, without the need for any external ligand or additive. The reactions were found to be scalable and to work even under partially aqueous conditions. This makes the reaction highly economical, practical, operationally simple and sustainable. The methodology provides direct access to π‐conjugated short oligomers consisting of furan, thiophene and indole. These compounds were found to show interesting fluorescence properties with remarkably large Stokes shift (up to 205 nm). Mechanistic investigations reveal that the reaction proceeds through chemoselective oxidation of indole by the metal catalyst followed by nucleophilic trapping by furan.
Deregulation of miR‐1245b‐5p and miR‐92a‐3p and their potential target gene, GATA3, in epithelial–mesenchymal transition pathway in breast cancer
Authors: Yadollahi Farsani Mahtab , Amini Farsani Zeinab , Teimuri Shohreh , Kolahdouzan Mohsen , Eshraghi Samani Reza , Teimori Hossein 
Published: January 2024

AbstractBackgroundMicroRNAs (miRNAs) are small molecules that have prominent roles in tumor development and metastasis and can be used for diagnostic and therapeutic purposes. This study evaluated the expression of miR‐92a‐3p and miR‐1245b‐5p and their potential target gene, GATA3 in patients with breast cancer (BC).Materials and MethodsIn the search for BC‐related microRNAs, miR‐124b‐5p and miR‐92a‐3p were selected using Medline through PubMed, miR2disease, miRcancer and miRTarBase. Moreover, target gene GATA3 and their possible interaction in the regulating epithelial‐mesenchymal transition (EMT) and invasion was evaluated using in silico tools including miRTarBase, TargetScan, STRING‐db, and Cytoscape. The expression level of miR‐92a‐3p, miR1245b‐5p, and GATA3 were assessed on extracted RNAs of tumor and nontumor tissues from 36 patients with BC using qPCR. Additionally, clinical‐pathologic characteristics, such as tumor grade, tumor stage, lymph node were taken into consideration and the diagnostic power of these miRNAs and GATA3 was evaluated using the ROC curve analysis.ResultsIn silico evaluation of miR‐92a‐3p and miR‐1245b‐5p supports their potential association with EMT and invasion signaling pathways in BC pathogenesis. Comparing tumor tissues to nontumor tissues, we found a significant downregulation of miR‐1245b‐5p and miR‐92a‐3p and upregulation of GATA3. Patients with BC who had decreased miR‐92a‐3p expression also had higher rates of advanced stage/grade and ER expression, whereas decreased miR‐1245b‐5p expression was only linked to ER expression and was not associated with lymph node metastasis. The AUC of miR‐1245b‐5p, miR‐92a‐3p, and GATA3 using ROC curve was determined 0.6449 (p = .0239), 0.5980 (p = .1526), and 0.7415 (p < .0001), respectively, which showed a significant diagnostic accuracy of miR‐1245b‐5p and GATA3 between the BC patients and healthy individuals.ConclusionMiR‐1245b‐5p, miR‐92a‐3p, and GATA3 gene contribute to BC pathogenesis and they may be having potential regulatory roles in signaling pathways involved in invasion and EMT pathways in BC pathogenesis, as a result of these findings. More research is needed to determine the regulatory mechanisms that they control.