bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Pharmacokinetics Reveal Impact of Lipid Composition on Microbubble and Nanoprogeny Shell Fate
Authors: Rajora Maneesha A. , Dhaliwal Alexander , Zheng Mark , Choi Victor , Overchuk Marta , Lou Jenny W. H. , Pellow Carly , Goertz David , Chen Juan , Zheng Gang 
Published: November 2023

AbstractMicrobubble‐enabled focused ultrasound (MB‐FUS) has revolutionized nano and molecular drug delivery capabilities. Yet, the absence of longitudinal, systematic, quantitative studies of microbubble shell pharmacokinetics hinders progress within the MB‐FUS field. Microbubble radiolabeling challenges contribute to this void. This barrier is overcome by developing a one‐pot, purification‐free copper chelation protocol able to stably radiolabel diverse porphyrin‐lipid‐containing Definity® analogues (pDefs) with >95% efficiency while maintaining microbubble physicochemical properties. Five tri‐modal (ultrasound‐, positron emission tomography (PET)‐, and fluorescent‐active) [64Cu]Cu‐pDefs are created with varying lipid acyl chain length and charge, representing the most prevalently studied microbubble compositions. In vitro, C16 chain length microbubbles yield 2–3x smaller nanoprogeny than C18 microbubbles post FUS. In vivo, [64Cu]Cu‐pDefs are tracked in healthy and 4T1 tumor‐bearing mice ± FUS over 48 h qualitatively through fluorescence imaging (to characterize particle disruption) and quantitatively through PET and γ‐counting. These studies reveal the impact of microbubble composition and FUS on microbubble dissolution rates, shell circulation, off‐target tissue retention (predominantly the liver and spleen), and FUS enhancement of tumor delivery. These findings yield pharmacokinetic microbubble structure‐activity relationships that disrupt conventional knowledge, the implications of which on MB‐FUS platform design, safety, and nanomedicine delivery are discussed.
Hydrogel Encapsulating Wormwood Essential Oil with Broad‐spectrum Antibacterial and Immunomodulatory Properties for Infected Diabetic Wound Healing
Authors: Wang Feng , Sun Qi , Li Yang , Xu Ruijun , Li Renjie , Wu Dingcai , Huang Rongkang , Yang Zifeng , Li Yong 
Published: November 2023

AbstractThe integration of hydrogels with bio‐friendly functional components through simple and efficient strategies to construct wound dressings with broad‐spectrum antibacterial and immunomodulatory properties to promote the healing of infected diabetic wounds is highly desirable but remains a major challenge. Here, wormwood essential oil (WEO) is effectively encapsulated in the hydrogel via an O/W‐Pickering emulsion during the polymerization of methacrylic anhydride gelatin (GelMA), acrylamide (AM), and acrylic acid N‐hydroxysuccinimide ester (AAc‐NHS) to form a multifunctional hydrogel dressing (HD‐WEO). Compared with conventional emulsions, Pickering emulsions not only improve the encapsulation stability of the WEO, but also enhance the tensile and swelling properties of hydrogel. The synergistic interaction of WEO's diverse bioactive components provides a broad‐spectrum antibacterial activity against S. aureus, E. coli, and MRSA. In addition, the HD‐WEO can induce the polarization of macrophages from M1 to M2 phenotype. With these advantages, the broad‐spectrum antibacterial and immunomodulatory HD‐WEO effectively promotes the collagen deposition and neovascularization, thereby accelerating the healing of MRSA‐infected diabetic wounds.
Fluorescence‐Quenching Lateral Flow Immunoassay for “Turn‐On” and Sensitive Detection of Anti‐SARS‐Cov‐2 Neutralizing Antibodies in Human Serum
Authors: Bian Lun , Fu Qiangqiang , Gan Zhuoheng , Wu Ze , Song Yuchen , Xiong Yufeng , Hu Fang , Zheng Lei 
Published: November 2023

AbstractThe titer of anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) neutralizing antibodies (NAbs) in the human body is an essential reference for evaluating the acquired protective immunity and resistance to SARS‐CoV‐2 infection. In this study, a fluorescence‐quenching lateral flow immunoassay (FQ‐LFIA) is established for quantitative detection of anti‐SARS‐CoV‐2 NAbs in the sera of individuals who are vaccinated or infected within 10 min. The ultrabright aggregation‐induced emission properties encapsulated in nanoparticles, AIE490NP, are applied in the established FQ‐LFIA with gold nanoparticles to achieve a fluorescence “turn‐on” competitive immunoassay. Under optimized conditions, the FQ‐LFIA quantitatively detected 103 positive and 50 negative human serum samples with a limit of detection (LoD) of 1.29 IU mL−1. A strong correlation is present with the conventional pseudovirus‐based virus neutralization test (R2 = 0.9796, P < 0.0001). In contrast, the traditional LFIA with a “turn‐off” mode can only achieve a LoD of 11.06 IU mL−1. The FQ‐LFIA showed excellent sensitivity to anti‐SARS‐CoV‐2 NAbs. The intra‐ and inter‐assay precisions of the established method are below 15%. The established FQ‐LFIA has promising potential as a rapid and quantitative method for detecting anti‐SARS‐CoV‐2 NAbs. FQ‐LFIA can also be used to detect various biomarkers.
Affinity‐Directed Site‐Specific Protein Labeling and Its Application to Antibody‐Drug Conjugates
Authors: Kim Sooin , Kim Sanggil , Kim Sangji , Kim Namkyoung , Lee Sang Won , Yi Hanbin , Lee Seungeun , Sim Taebo , Kwon Yongseok , Lee Hyun Soo 
Published: November 2023

AbstractChemically modified proteins have diverse applications; however, conventional chemo‐selective methods often yield heterogeneously labeled products. To address this limitation, site‐specific protein labeling holds significant potential, driving extensive research in this area. Nevertheless, site‐specific modification of native proteins remains challenging owing to the complexity of their functional groups. Therefore, a method for site‐selective labeling of intact proteins is aimed to design. In this study, a novel approach to traceless affinity‐directed intact protein labeling is established, which leverages small binding proteins and genetic code expansion technology. By applying this method, a site‐specific antibody labeling with a drug, which leads to the production of highly effective antibody‐drug conjugates specifically targeting breast cancer cell lines is achieved. This approach enables traceless conjugation of intact target proteins, which is a critical advantage in pharmaceutical applications. Furthermore, small helical binding proteins can be easily engineered for various target proteins, thereby expanding their potential applications in diverse fields. This innovative approach represents a significant advancement in site‐specific modification of native proteins, including antibodies. It also bears immense potential for facilitating the development of therapeutic agents for various diseases.
A Near‐Infrared Fluorogenic Probe for Rapid, Specific, and Ultrasensitive Detection of Sphingosine in Living Cells and In Vivo
Authors: Chen Yanyan , Hao Tingting , Wang Jing , Chen Yiming , Wang Xiuxiu , Wei Wei , Zhao Jing , Qian Yong 
Published: November 2023

AbstractSphingosine (Sph) plays important roles in various complex biological processes. Abnormalities in Sph metabolism can result in various diseases, including neurodegenerative disorders. However, due to the lack of rapid and accurate detection methods, understanding sph metabolic in related diseases is limited. Herein, a series of near‐infrared fluorogenic probesDMS‐X(X = 2F, F, Cl, Br, and I) are designed and synthesized. The fast oxazolidinone ring formation enables theDMS‐2Fto detect Sph selectively and ultrasensitively, and the detection limit reaches 9.33 ± 0.41 nm. Moreover, it is demonstrated thatDMS‐2Fexhibited a dose‐ and time‐dependent response to Sph and can detect sph in living cells. Importantly, for the first time, the changes in Sph levels induced by Aβ42oligomers and H2O2are assessed through a fluorescent imaging approach, and further validated the physiological processes by which Aβ42oligomers and reactive oxygen species (ROS)‐induce changes in intracellular Sph levels. Additionally, the distribution of Sph in living zebrafish is successfully mapped by in vivo imaging of a zebrafish model. This work provides a simple and efficient method for probing Sph in living cells and in vivo, which will facilitate investigation into the metabolic process of Sph and the connection between Sph and disease pathologies.
Hybridization in agricultural weeds: A review from ecological, evolutionary, and management perspectives
Authors: Westbrook Anna S. , DiTommaso Antonio 
Published: November 2023

AbstractAgricultural weeds frequently hybridize with each other or with related crop species. Some hybrid weeds exhibit heterosis (hybrid vigor), which may be stabilized through mechanisms like genome duplication or vegetative reproduction. Even when heterosis is not stabilized, hybridization events diversify weed gene pools and often enable adaptive introgression. Consequently, hybridization may promote weed evolution and exacerbate weed–crop competition. However, hybridization does not always increase weediness. Even when viable and fertile, hybrid weeds sometimes prove unsuccessful in crop fields. This review provides an overview of weed hybridization and its management implications. We describe intrinsic and extrinsic factors that influence hybrid fitness in agroecosystems. We also survey the rapidly growing literature on crop–weed hybridization and the link between hybridization and invasiveness. These topics are increasingly relevant in this era of genetic tools for crop improvement, intensive and simplified cropping systems, and globalized trade. The review concludes with suggested research priorities, including hybridization in the context of climate change, plant–insect interactions, and redesigned weed management programs. From a weed management perspective, hybridization is one of many reasons that researchers and land managers must diversify their weed control toolkits.
First fossil woods and palm stems from the mid‐Paleocene of Myanmar and implications for biogeography and wood anatomy
Authors: Gentis Nicolas , Licht Alexis , De Franceschi Dario , Win Zaw , Aung Day Wa , Dupont‐Nivet Guillaume , Boura Anaïs 
Published: November 2023

AbstractPremiseThe rise of angiosperm‐dominated tropical rainforests has been proposed to have occurred shortly after the Cretaceous–Paleogene transition. Paleocene fossil wood assemblages are rare yet provide important data for understanding these forests and whether their wood anatomical features can be used to document the changes that occurred during this transition.MethodsWe used standard techniques to section 11 fossil wood specimens of Paleocene‐age, described the anatomy using standard terminology, and investigated their affinities to present‐day taxa.ResultsWe report here the first middle Paleocene fossil wood specimens from Myanmar, which at the time was near the equator and anchored to India. Some fossils share affinities with Arecaceae, Sapindales (Anacardiaceae, Meliaceae) and Moraceae and possibly Fabaceae or Lauraceae. One specimen is described as a new species and genus: Compitoxylon paleocenicum gen. et sp. nov.ConclusionsThis assemblage reveals the long‐lasting presence of these aforementioned groups in South Asia and suggests the early presence of multiple taxa of Laurasian affinity in Myanmar and India. The wood anatomical features of the dicotyledonous specimens reveal that both “modern” and “primitive” features (in a Baileyan scheme) are present with proportions similar to features in specimens from Paleocene Indian localities. Their anatomical diversity corroborates that tropical flora display “modern” features early in the history of angiosperms and that their high diversity remained steady afterward.
Gamete sex and elevation affect genetically based variation for unreduced gamete production in a mixed‐ploidy plant
Authors: DeVries Sarah , Kron Paul , Husband Brian C. 
Published: November 2023

AbstractPremiseUnreduced gametes are the primary mechanism of neopolyploid formation. Their production in diploid populations is arguably maladaptive, but the magnitude and patterns of genetically based variation maintained in natural populations are poorly understood.MethodsWe examined variation in male and female unreduced gamete production among plants from different elevations in fireweed, Chamerion angustifolium, grown in a common environment. Using seeds from three high‐elevation and three low‐elevation diploid populations in one study, and a single diploid population in another, we estimated realized rates of unreduced male (sperm) and female (egg) gamete production by reciprocally pollinating diploid and tetraploid plants and estimating the incidence of tetraploid seeds using flow cytometry.ResultsUnreduced gamete frequencies per plant were similar in the two studies (0.12% vs. 0.08%). High‐elevation populations had a greater percentage of fruit with seeds from unreduced gametes, but a lower percentage of seeds per fruit than low‐elevation populations. Female unreduced gamete frequencies differed among elevations, but male frequencies did not, and the gamete sexes were not correlated at the plant level.ConclusionsWe conclude that genetically based variation for unreduced gametes is maintained within and among natural populations, despite their fitness disadvantages, suggesting that local selection may be ineffective at purging them under some conditions.
Brain metabolic network covariance and aging in a mouse model of Alzheimer's disease
Authors: Chumin Evgeny J. , Burton Charles P. , Silvola Rebecca , Miner Ethan W. , Persohn Scott C. , Veronese Mattia , Territo Paul R. 
Published: November 2023

AbstractINTRODUCTIONAlzheimer's disease (AD), the leading cause of dementia worldwide, represents a human and financial impact for which few effective drugs exist to treat the disease. Advances in molecular imaging have enabled assessment of cerebral glycolytic metabolism, and network modeling of brain region have linked to alterations in metabolic activity to AD stage.METHODSWe performed 18F‐FDG positron emission tomography (PET) imaging in 4‐, 6‐, and 12‐month‐old 5XFAD and littermate controls (WT) of both sexes and analyzed region data via brain metabolic covariance analysis.RESULTSThe 5XFAD model mice showed age‐related changes in glucose uptake relative to WT mice. Analysis of community structure of covariance networks was different across age and sex, with a disruption of metabolic coupling in the 5XFAD model.DISCUSSIONThe current study replicates clinical AD findings and indicates that metabolic network covariance modeling provides a translational tool to assess disease progression in AD models.
Performance optimization of acrylonitrile butadiene styrene/thermoplastic polyurethane composite foams blown with carbon dioxide using Taguchi technique
Authors: Khaleghi Sara , Azdast Taher , Doniavi Ali , Hasanzadeh Rezgar 
Published: November 2023

AbstractIn this study, acrylonitrile butadiene styrene (ABS)/thermoplastic polyurethane (TPU) composite foam blown with CO2 was fabricated. Optimization was done by design of experiment (DOE) on the cellular structure using the Taguchi method. Foaming time (20, 40, and 80 s), saturation pressure (4, 5.5, and 7 MPa), and foaming temperature (80, 90, and 120°C) are the input parameters. The results obtained from the signal‐to‐noise (S/N) analysis showed that the most effective factor on the cell density (CD) was the saturation pressure and its influence rate was 48.05%, and also, the CD improved with the increase in the saturation pressure because the high saturation pressure leads to an enhancement in gas solubility and the rate of cell nucleation. Moreover, the foaming temperature and the foaming time had a noteworthy impact on the void fraction and the cell size (CS), and they should be controlled accurately. The impression rate of the foaming time on the CS was 50.86%, and also, with increase in the temperature and the time of foaming, the void fraction showed an increasing trend. The optimal values for the CD, the CS, and the void fraction were predicted to be 1.18 × 109 cells/cm3, 5.37 μm, and 0.5744%, respectively.
Can you change my generosity towards future others? The impact of observability on intertemporal pro‐social decisions
Authors: Hong Mei , Liang Dapeng , Lu Teng 
Published: November 2023

AbstractResearch has demonstrated that delays in realizing pro‐social decisions significantly influence pro‐social choices. However, the impact of time delay may vary by context. A key contextual factor is decision observability (i.e., the visibility of one's decision to others). Using a dictator game task with delayed rewards, the current study examined the effects of observability on intertemporal pro‐social choices. Subjects were randomly assigned to either an Anonymous group, where payment was private, or an Observable group, involving public payment. They had to decide between the selfish option (which only benefited the subject) and the generous option (sharing money with another person in a specific delay condition). Our data revealed that subjects were less eager to forgo money when time delay increased and showed more selfishness toward specific people, independent of decision observability. This pattern was aligned with a hyperbolic discounting model. Notably, observability mitigated the impact of time delay; subjects were more inclined to donate to temporally distant individuals when their decisions were observable instead of anonymous. In addition, we discuss the practical implications of observability for designing intertemporal donation appeals.
“It wasn't me, check the cameras!” Suspects' apparent verifiable responses might not indicate innocence
Authors: Jiménez‐González Ana Isabel , Masip Jaume , Blandón‐Gitlin Iris , Herrero Carmen 
Published: November 2023

AbstractThe verifiability of a suspect's alibi is often interpreted as a sign of innocence. Because the police resources are limited, verifiability could be used to dismiss suspects of minor offenses. We examined whether alibi verifiability actually indicates innocence for minor crimes. In Experiment 1, participants imagined they were guilty or innocent suspects of minor crimes and selected a response to convince the police of their innocence. Compared to innocent suspects, guilty suspects were more likely to select pseudo‐verifiable responses (which seemed verifiable but were not) rather than non‐verifiable responses. Experiment 2 revealed that pseudo‐verifiable responses increased observers' perceptions of innocence (rather than guilt). Experiment 3 suggested that people infer the police will not verify alibis of minor crimes, which may lead people to invent pseudo‐verifiable responses. These results indicate that apparent verifiability does not necessarily indicate innocence. The police should systematically test alternative hypotheses whenever they encounter apparent verifiable responses.
Definition and anatomical description of the left atrial appendage neck
Authors: Batko Jakub , Jakiel Rafał , Krawczyk–Ożóg Agata , Litwinowicz Radosław , Hołda Jakub , Bartuś Stanisław , Bartuś Krzysztof , Hołda Mateusz K. , Konieczyńska Małgorzata 
Published: November 2023

AbstractThe left atrial appendage (LAA) is well known as a source of cardiac thrombus formation. Despite its clinical importance, the LAA neck is still anatomically poorly defined. Therefore, this study aimed to define the LAA neck and determine its morphometric characteristics. We performed three‐dimensional reconstructions of the heart chambers based on contrast‐enhanced electrocardiography–gated computed tomography scans of 200 patients (47% females, 66.5 ± 13.6 years old). The LAA neck was defined as a truncated cone‐shaped canal bounded proximally by the LAA orifice and distally by the lobe origin and was present in 98.0% of cases. The central axis of the LAA neck was 14.7 ± 2.3 mm. The mean area of the LAA neck walls was 856.6 ± 316.7 mm2. The LAA neck can be divided into aortic, arterial (the smallest), venous (the largest), and free surfaces. All areas have a trapezoidal shape with a broader proximal base. There were no statistically significant differences in the morphometric characteristics of the LAA neck between LAA types. Statistically significant differences between the sexes in the main morphometric parameters of the LAA neck were found in the central axis length and the LAA neck wall area. The LAA neck can be evaluated from computed tomography scans and their three‐dimensional reconstructions. The current study provides a complex morphometric analysis of the LAA neck. The precise definition and morphometric details of the LAA neck presented in this study may influence the effectiveness and safety of LAA exclusion procedures.
Synthesis of High‐Purity Monocalcium Phosphate Monohydrate from Crab Shell Waste
Authors: Pusparizkita Yustina M. , Schmahl Wolfgang W. , Widiyanto Muhammad A. , Ismail Rifky , Jamari J. , Bayuseno Athanasius P. 
Published: November 2023

AbstractWaste crab shells (CS) have the potential to mineralize CO2, yielding precipitated calcium carbonate (PCC) powder feedstock for use in the synthesis of monocalcium phosphate monohydrate (MCPM) in an acidic condition by partial neutralization of phosphoric acid at 90 °C and varying times (30–120 min), followed by evaporation of the water at 100 °C. The PCC and MCPM products were analyzed using X‐ray powder diffraction, Fourier transform infrared spectroscopy, and the scanning electron microscopy/energy dispersive X‐ray method. After 120 min of stirring, the pure MCPM revealed uniform particle sizes from 1 to 3 μm. The presented MCPM synthesis using the PCC powder precursor may assist in developing a large‐scale MCPM industry while recycling a considerable quantity of CS waste.
Remarkable Off–On Tunable Solid‐State Luminescence by the Regulation of Pyrene Dimer
Authors: Zhao Wenyang , Ding Zeyang , Yang Zhiqiang , Lu Tong , Yang Bing , Jiang Shimei 
Published: November 2023

AbstractIt is always a challenge to achieve “off–on” luminescent switch by regulating non‐covalent interactions. Herein, we report a unique strategy for constructing high performance “off–on” tunable luminescent materials utilizing a novel molecule (TFPA) consist of pyrene and cyanostilbene. The pristine crystal of TFPA is almost non‐emissive. Upon grinding/UV irradiation, an obvious luminescence enhancement is observed. Theoretical and experimental results revealed the underlying mechanism of this intriguing “off–on“ switching behavior. The non‐emissive crystal consists of ordered H‐aggregates, with adjacent two molecules stacked in an anti‐parallel manner and no overlapped area in pyrene moieties. When external force is applied by grinding or internal force is introduced through the photoisomerization, the dimer structures are facilitated with shorter intermolecular distances and better overlapping of pyrene moieties. In addition, the “on” state can recover to “off” state under thermal annealing, showing good reversibility and applicability in intelligence material. The present results promote an in‐depth insight between packing structure and photophysical property, and offer an effective strategy for the construction of luminescence “off–on” switching materials, toward the development of stimuli‐responsive luminescent materials for anti‐counterfeiting.