bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of MMPs by soluble E‐cadherin in human lung tumor cells
Authors: Nawrocki‐Raby Béatrice , Gilles Christine , Polette Myriam , Bruyneel Erik , Laronze Jean‐Yves , Bonnet Noël , Foidart Jean‐Michel , Mareel Marc , Birembaut Philippe 
Published: May 2003

AbstractLoss of E‐cadherin/catenin mediated cell–cell adhesion and overexpression of matrix metalloproteinases (MMPs) are largely involved in tumor invasion. It has been recently shown that high levels of a soluble 80 kDa fragment of E‐cadherin, resulting from a cleavage by MMPs, are found in serum and in urine from cancer patients. Additionally, this soluble E‐cadherin (sE‐CAD) promotes cell invasion into chick heart and into collagen type I gels. The aim of our study was to examine the mechanism of sE‐CAD‐induced cell invasion. Since MMPs play a crucial role in invasion, we looked for induction of MMPs by sE‐CAD in noninvasive human lung tumor cells 16HBE. An induction of MMP‐2, MMP‐9 and MT1‐MMP expression was observed both at the mRNA and at the protein level in the presence of sE‐CAD (in conditioned medium form or in E‐cadherin HAV peptide form). No induction of MMP‐1, ‐3 and ‐7 or variation of the levels of their inhibitors, TIMP‐1 and TIMP‐2, were detected. The biologic relevance of the sE‐CAD‐induced MMP upregulation was tested by demonstrating that sE‐CAD promotes in vitro cell invasion in a modified Boyden chamber assay. These data provide new insight into mechanisms of tumor invasion by ectodomain shedding of the cell–cell adhesion molecule E‐cadherin. © 2003 Wiley‐Liss, Inc.
Nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1) is induced by genistein through the expression of p53 in colorectal cancer cells
Authors: Wilson Leigh C. , Baek Seung Joon , Call Allison , Eling Thomas E. 
Published: May 2003

AbstractGenistein is an isoflavenoid found in soy that has anti‐tumorigenic activities. Treatment of colorectal carcinoma HCT‐116 cells with 50 μM genistein results in a 50% reduction in cell proliferation and a 6‐fold increase in apoptosis. Genistein induces nonsteroidal anti‐inflammatory drug‐activated gene 1 (NAG‐1), a protein with antitumorigenic activities, in a time‐ and concentration‐dependent manner in HCT‐116 cells. In addition, p53 and p21 are induced in HCT‐116 cells. The induction of p53 (3 hr) precedes the induction of NAG‐1 (12 hr), suggesting that genistein‐induced NAG‐1 expression is mediated by p53. In contrast, NAG‐1 is not induced by genistein in the p53‐negative colorectal carcinoma cell line HCT‐15. Luciferase reporter constructs of the NAG‐1 promoter containing 2 p53 sites showed that the p53 sites within the NAG‐1 promoter are critical to genistein‐induced NAG‐1 expression in p53‐positive U2OS cells. The expression of p53 was critical for NAG‐1 promoter activity since no promoter activity was observed with genistein treatment in HCT‐15 cells. However, genistein‐induced promoter activity was restored in HCT‐15 cells by transfection with wild‐type p53. Together our data suggest a relationship between genistein, p53 and NAG‐1 forming a novel pathway responsible for the antitumorigenic activity of genistein. © 2003 Wiley‐Liss, Inc.
Increased adhesion potency of ovarian carcinoma cells to mesothelial cells by overexpression of dipeptidyl peptidase IV
Authors: Kikkawa Fumitaka , Kajiyama Hiroaki , Ino Kazuhiko , Shibata Kiyosumi , Mizutani Shigehiko 
Published: May 2003

AbstractDipeptidyl peptidase IV (DPPIV), cell surface peptidase, works as an adhesion molecule as well as an enzyme. To investigate the role of DPPIV as an adhesion molecule of ovarian carcinoma cells to mesothelial cells, ovarian carcinoma (SKOV3 and NOS4) cells were transfected with a sense and an antisense cDNA coding human DPPIV. The adhesion potency of these transfected cells to fibronectin‐ and collagen‐coated plates and mesothelial cells were examined with or without fibronectin. Flow cytometry and immunohistochemistry demonstrated DPPIV in ovarian carcinoma cells. The adhesion rate of DPPIV‐transfected SKOV3 (SKDPIV) cells to fibronectin‐coated plates was significantly higher than SKOV3 cells, while there was no difference in the adhesion rate to non‐coated plates between SKDPIV and SKOV3 cells. The adhesion rates of vector‐transfected SKOV3 (SKpcDNA) cells to coating and noncoating plates were similar to those of SKOV3 cells. SKDPIV cells showed twofold adhesion potency to mesothelial cells compared to SKOV3 cells. Furthermore, an addition of soluble fibronectin resulted in a dose‐dependent increase in the adhesion rate of SKDPPIV cells, but not in either SKOV3 or SKpcDNA cells. Antisense‐transfected NOS4 cells decreased the adhesion potency compared to NOS4 and vector‐transfected NOS4 cells. In conclusion, ovarian carcinoma cells express DPPIV and adhere to human mesothelial cells in part by DPPIV. This adhesion mechanism of DPPIV is mediated by immobilized and soluble fibronectin. © 2003 Wiley‐Liss, Inc.
Novel plant triterpenoid drug amooranin overcomes multidrug resistance in human leukemia and colon carcinoma cell lines
Authors: Ramachandran Cheppail , Rabi Thangaiyan , Fonseca Hugo B. , Melnick Steven J. , Escalon Enrique A. 
Published: May 2003

AbstractAmooranin (AMR), a plant terpenoid, isolated from Amoora rohituka, was investigated for its ability to overcome multidrug resistance in human leukemia and colon carcinoma cell lines. AMR IC50 values of multidrug‐resistant leukemia (CEM/VLB) and colon carcinoma (SW620/Ad‐300) cell lines were higher (1.9‐ and 6‐fold) than parental sensitive cell lines (CEM and SW620). AMR induced G2+M phase‐arrest during cell cycle traverse in leukemia and colon carcinoma cell lines and the percentage of cells in G2+M phase increased in a dose‐dependent manner. Coincubation of tumor cells with both DOX and AMR reversed DOX resistance in 104‐fold DOX‐resistant CEM/VLB and 111‐fold DOX‐resistant SW620/Ad‐300 cell lines with a dose modification factor of 50.9 and 99.6, respectively. Flow cytometric assay showed that AMR causes enhanced cellular DOX accumulation in a dose‐dependent manner. AMR inhibits photolabeling of P‐glycoprotein (P‐gp) with [3H]‐azidopine and the blocking effect enhanced with increasing concentrations of AMR. Our results show that AMR competitively inhibits P‐gp‐mediated DOX efflux, suggestive of a mechanism underlying the enhanced DOX accumulation and reversal of multidrug resistance by AMR. © 2003 Wiley‐Liss, Inc.
[125I], [127I]‐and [14C]‐Labelling of the GLP‐1‐(7‐37) derivative NN2211
Authors: Kristensen Jesper B. , Pedersen Mette L. , Larsen Ulla D. , Martiny Lars , Hansen Louis B. , Foged Christian 
Published: May 2003

AbstractArg34Lys26(Nε‐(γ‐L‐glutamyl(Nα‐palmitoyl)))‐GLP‐1(7‐37) (NN2211) is currently in development as a diabetes type 2 drug. The fatty acid attached to the GLP‐1(7‐37) ensures a long and controlled duration of action. The synthesis of [125I]NN2211, [127I]NN2211 and [14C]NN2211 used for preclinical ADME studies are described. NN2211 was iodinated using the lactoperoxidase/hydrogen peroxide method, and [14C]NN2211 was synthesized in 4 steps by two routes both starting from an α‐protected [U‐14C]glutamic acid. Copyright © 2003 John Wiley & Sons, Ltd.
Iridium‐mediated β‐deuteration of enones
Authors: Gibson Jennifer S. , Herbert John M. 
Published: May 2003

AbstractExposure of captodative enone systems to deuterium in the presence of Crabtree's catalyst (1) results in deuteration at the vinylic site β‐ to the ketone carbonyl, as well as at any accessible ortho‐position. β‐exchange is also observed during the reduction of ethyl cinnamate (3) catalyzed by 1. Copyright © 2003 John Wiley & Sons, Ltd.
How to increase the reactivity of [18F]fluoroethyl bromide: [18F]fluoroethylation of amine, phenol and amide functional groups with [18F]FEtBr, [18F]FEtBr/NaI and [18F]FEtOTf
Authors: Zhang Ming‐Rong , Furutsuka Kenji , Yoshida Yuichiro , Suzuki Kazutoshi 
Published: May 2003

Abstract[18F]Fluoroethyl bromide ([18F]FEtBr) is a useful synthetic precursor to synthesize 18F‐labeled compounds. However, the lower reactivity of [18F]FEtBr with amine, phenol and amide functional groups than that of [11C]CH3I partly limits its wide application in the synthesis of [18F]fluoroethylated compounds. The aim of this study was to increase the reactivity of [18F]FEtBr with various nucleophilic substrates for PET tracers containing amine, phenol and amide moieties. The present strategies included (1) adding NaI into the reaction mixture of [18F]FEtBr and substrate, where [18F]FEtI is reversibly formed and becomes more reactive; (2) converting [18F]FEtBr into much more reactive [18F]FEtOTf, similar to conversion of [11C]CH3I into [11C]CH3OTf. By these efforts, the [18F]fluoroethylation efficiency of various substrates containing amine, phenol and amide groups with [18F]FEtBr/NaI and [18F]FEtOTf was significantly improved, compared with the corresponding reaction efficiency with [18F]FEtBr. Copyright © 2003 John Wiley & Sons, Ltd.
Temperature controlled burn generation system based on a CO2 laser and a silver halide fiber optic radiometer
Authors: Cohen Meir , Ravid Avi , Scharf Vered , Hauben Daniel , Katzir Abraham 
Published: May 2003

AbstractBackground and ObjectivesExperimental animal study of burns is dependent on a reliable burn generation system. Most of the experimental systems used today are unable to produce precise partial thickness burns. This limits the ability to study minor changes associated with burn care. The aim of the study was to develop a method for generating burns with a fixed depth using a CO2 laser burn generation system.Materials and MethodsThe burn generation system was composed of two components: a burn generation device and a temperature sensing and control system. These components were designed to operate together in order to keep a constant, predetermined skin surface temperature during prolonged burn generation. One hundred thirty‐eight spot burns were generated on the back of five shaved 450 g male Wistar rats. The rat skin was exposed to a 70°C for 5–60 seconds. The burned areas were excised and underwent evaluation by hematoxylin‐eosin‐stained slide microscopy.ResultsA linear correlation was found between the duration of exposure and the average burn depth (r = 0.93). This correlation is represented by the equation: burn depth in millimeters = 0.012× (duration in seconds of skin exposure at 70°C).ConclusionsThe fiber‐optic‐controlled laser burn generation system studied is a reliable tool for creating partial thickness as well as full thickness skin burns in rats. Lasers Surg. Med. 32:413–416, 2003. © 2003 Wiley‐Liss, Inc.
Use of Q‐switched ruby laser in the treatment of nevus of ota in different age groups
Authors: Kono Taro , Chan Henry H.L. , Erçöçen Ali Riza , Kikuchi Yuji , Uezono Shoichi , Iwasaka Susumu , Isago Tsukasa , Nozaki Motohiro 
Published: May 2003

AbstractBackground and ObjectivesNevus of Ota is a form of dermal melanocytic hamartoma that appears as a bluish discoloration in the trigeminal region. Although Q‐switched lasers provide effective treatment, the appropriate age at which to start that treatment is not known. Our aim is to compare the clinical efficacy and safety of Q‐switched ruby laser (QSRL) in the treatment of nevus of Ota in different age groups.Study Design/Materials and MethodsOur study included 46 children and 107 adults with nevus of Ota, which had been treated with QSRL that achieved excellent (75% or more) to complete response. The laser parameters were 694‐nm wavelength, 30 nanoseconds pulse duration, 4‐mm spot size, and 5–7 J/cm2 fluence at 3–4 month intervals. We assessed the mean number of treatment sessions and the degree of complications in the two age groups of patients.ResultsThe mean number of treatment sessions to achieve significance to complete clearing was 3.5 for the younger age group and 5.9 for the older age group (P = 0.0001). The complication rate for the younger age group was 4.8% as compared to 22.4% for the older age group.ConclusionsThe use of QSRL for the treatment of nevus of Ota in children can achieve an excellent result in fewer sessions and at a lower complication rate than later treatment. The risk of recurrence is a concern, however, and further long‐term study is necessary to address this issue. Lasers Surg. Med. 32:391–395, 2003. © 2003 Wiley‐Liss, Inc.
Facial rhytides—subsurfacing or resurfacing? A review
Authors: Grema Hortensia , Greve Bärbel , Raulin Christian 
Published: May 2003

AbstractStudy Design/Background and ObjectivesCurrently, ablative laser therapy (with CO2/Er:YAG lasers) is considered an effective and promising method of skin rejuvenation. The induction of collagen synthesis was observed after treatments with the CO2laser and with the long‐pulsed Er:Yag laser. In past years, the undesirable side effects and risks of these methods have led to intensified research efforts in the fields of non‐ablative facial rejuvenation as well as subsurfacing by means of non‐ablative laser systems and intense pulsed light systems. The objective is to achieve selective, heat‐induced denaturalization of dermal collagen that leads to subsequent reactive synthesis of neocollagen but does not damage the epidermis. This article reviews the use of different types of lasers and intense pulsed light sources for the non‐ablative treatment of facial rhytides.ResultsThe results of numerous clinical and histological investigations have recently indicated that these new technologies are successful. Some studies demonstrated remarkable effects with non‐ablative systems; others, however, showed only limited cosmetic improvement or none at all.ConclusionsAfter critical review and assessment of current literature on the treatment of rhytides, we have found that non‐ablative methods do not appear to be a comparable alternative to ablative skin resurfacing in terms of their efficacy and side effects. Lasers Surg. Med. 32:405–412, 2003. © 2003 Wiley‐Liss, Inc.
A preliminary study of laser tissue soldering as arterial wall reinforcement in an acute experimental aneurysm model
Authors: Oskoui Philip , Stadler Istvan , Lanzafame Raymond J. 
Published: May 2003

AbstractBackground and ObjectivesAneurysm formation results from destruction of structural arterial wall connective tissue, leading to wall weakening and rupture. The purpose of this study was to demonstrate that reinforcement of the arterial wall using laser tissue soldering contributes to arterial wall stabilization and rupture prevention in an acute experimental model.Study Design/Materials and MethodsElastase (10 U/mg protein, Sigma‐Aldrich Co., St. Louis, MO) was applied with a fine paint brush on femoral artery segments to cause fusiform aneurysm formation. After aneurysms formed (approximately 45 minutes after treatment), elastase was rinsed out and indocyanine green (ICG) and albumin soldering mixture (2.5 mg/ml ICG in 50% albumin) was delivered to the arterial segment, followed by laser irradiation at 830 nm, (15mW output for 20 minutes). In situ pressure burst measurements were then performed.ResultsIn situ burst pressures were > 503 mmHg for normal arteries and 181 ± 26.0 mmHg, for Elastase treated segments. (P < 0.0001) Treatment of experimental aneurysms laser tissue soldering returned burst strengths to > 503 mmHg.ConclusionsThese results indicate laser tissue soldering reinforcement of weak arterial walls, is possible and may reduce the likelihood of acute rupture. Further development of this technique for aneurysm management is warranted. Lasers Surg. Med. 32:346–348, 2003. © 2003 Wiley‐Liss, Inc.
Value of endotracheal tube safety in laryngeal laser surgery
Authors: Sesterhenn Andreas M. , Dünne Anja‐A. , Braulke Daniel , Lippert Burkard M. , Folz Benedikt J. , Werner Jochen A. 
Published: May 2003

AbstractBackground and ObjectivesDue to the increased popularity of laryngeal laser therapy, surgeons and anesthesiologists are inevitably confronted with questions concerning the choice of the most efficient endotracheal tube (ETT) for laryngeal laser surgery, especially with regard to possible endolaryngeal tube fires, or combustions. The purpose of this study was to determine the current practice in endolaryngeal laser surgery in Germany.Study Design/Materials and MethodsA questionnaire was sent to 152 ENT Departments in Germany, care was taken that the responders could send back the questionnaire anonymously. Among other questions the participants were asked for the number of lasersurgical treatments of the larynx performed in the past, the usual type of ETTs in use, whether other safety precautions were taken during CO2 laser surgery of the larynx and for intraoperative complications like tube ignition, fires or combustions.ResultsEighty six of the 152 addressed ENT departments replied. In laryngeal laser surgery, 59/86 departments regularly use special laser tubes in daily routine (74.5%). In about 20,000 lasersurgical procedures, 15 incidents of ETT fire have been reported. In six of the reported 15 cases a tube fire occurred despite the fact that special laser tubes had been utilized.ConclusionsThe present study could demonstrate that the use of special laser tubes does not necessarily protect against ETT fire. Thus, even when using special laser tubes other safety measures should be taken. In view of the maximum safety for the patient it has to be stated, that the safety during surgery correlates definitely with the experience of the surgeon. The weakest point of ETTs is usually situated in the cuff region. Lasers Surg. Med. 32:384–390, 2003. © 2003 Wiley‐Liss, Inc.
Hair removal with long pulsed diode lasers: A comparison between two systems with different pulse structures
Authors: Fiskerstrand Eli Janne , Svaasand Lars O. , Nelson J. Stuart 
Published: May 2003

AbstractBackground and ObjectivesThe aim was to study hair removal efficacy, and possible side effects of two commercially available long pulsed diode lasers. The radiant exposure was selected to a value of 35 J/cm2, which is frequently used in the clinic in accordance with manufacturer's recommendations.Study Design/Materials and MethodsA prospective clinical study was performed on twenty‐nine patients with hair color ranging from light brown to black on the upper lip. One half of the upper lip was randomly selected for treatment with the MedioStar® laser; the contralateral half of the lip was treated with the LightSheer® laser. Three treatments were performed at 6–8 week intervals. Percent hair reduction and acute‐ and long‐term side effects were evaluated after treatment.ResultsThe average hair reductions 6 months after the first treatment were 49% with the MedioStar® laser and 48% with the LightSheer® laser. No scarring or pigmentary change of the skin was observed after any of the treatments with either laser. However, differences in acute side effects such as degree of erythema and burned hairs were observed.ConclusionsNo statistically significant differences in hair removal efficacy were observed. These results agree with mathematical modeling, which also offers a method to estimate hair removal efficacy and adverse effects for a range of hair characteristics and laser parameters. Lasers Surg. Med. 32:399–404, 2003. © 2003 Wiley‐Liss, Inc.
Measurement of the elastic modulus of rabbit nasal septal cartilage during Nd:YAG (λ = 1.32 μm) laser irradiation
Authors: Chao Kenneth K.H. , Ho Ki‐Hong Kevin , Wong Brian J.F. 
Published: May 2003

AbstractBackground and ObjectivesThe objective of this study was to quantitatively measure changes in the elastic moduli of rabbit nasal septal cartilage during laser heating. While the efficacy of laser cartilage reshaping has been established for use in nasal surgery, few studies have investigated the temperature‐dependent viscoelastic behavior of cartilage.Study Design/Materials and MethodsCyclic force versus displacement curves were generated during the Nd:YAG laser (λ = 1.32 μm, 10 second exposure time, 21.22 W/cm2) irradiation of cartilage specimens secured in cantilevered geometry. Samples were irradiated three times with 30 second cooling intervals between each laser exposure. Measurements were recorded before, during, and after laser irradiation, and then following complete rehydration in normal saline (NS) for 1 hour at 25°C. Elastic modulus was calculated assuming linear viscoelastic behavior.ResultsThe elastic modulus in native tissue decreased during and after successive laser exposures from about 6 to 3.5 MPa. After rehydration, the modulus returned to near‐baseline value. Surface temperature reached a maximum of 65°C.ConclusionsThe laser irradiation of cartilage using parameters similar to those used in reshaping does not produce significant irreversible changes in the mechanical properties of the tissue. Measurement of the elastic modulus is an effective means of characterizing alterations in cartilage mechanical behavior during and after laser heating. Lasers Surg. Med. 32:377–383, 2003. © 2003 Wiley‐Liss, Inc.
Preliminary study of laser welding for aortic dissection in a porcine model using a diode laser with indocyanine green
Authors: Fujita Masanori , Morimoto Yuji , Ohmori Sayaka , Usami Noriko , Arai Tsunenori , Maehara Tadaaki , Kikuchi Makoto 
Published: May 2003

AbstractBackground and ObjectivesThe objective of this study was to determine whether a dissected aorta could be welded by a diode laser with a solder using an in vitro porcine aortic dissection model.Study Design/Materials and MethodsPorcine aortic strips were dissected into two flaps and the dissected faces were immersed in a solution of indocyanine green. The two flaps were pressed at 0.2 kg/cm2 with contact between the two immersed faces. The pressed flaps were irradiated with a diode laser (810 nm) at intensities of 170–425 W/cm2 for 8 seconds. The welded flaps were studied by light microscopy and the adhesive strengths were measured.ResultsThe irradiated flaps were successfully welded. The breaking stress, the maximum stress recorded in a stress‐strain curve, increased with increase in irradiation intensity up to 396 W/cm2 (2.7 × 102 mmHg) and decreased when the intensity reached 425 W/cm2. In the specimen irradiated at 396 W/cm2, the welded faces showed continuous fusion of elastin layers, while some voids were seen between the welded faces in the specimen irradiated at 425 W/cm2.ConclusionsThe dissected porcine aortas were successfully welded using a laser with solder. The results suggest that the welded aorta can bear physiological blood pressure. Lasers Surg. Med. 32:341–345, 2003. © 2003 Wiley‐Liss, Inc.
Leg ulceration after pulsed dye laser treatment of a vascular malformation
Authors: Lamb Steven R. , Sheehan‐Dare Robert A. 
Published: May 2003

AbstractBackground and ObjectivesThe pulse dye laser (PDL) is a well‐established treatment for port wine stains (PWSs) and is generally accepted to be safe, effective, and results in fading in the majority of patients. Significant side effects such as scarring are extremely rare. To date ulceration has not been reported.Study Design/Patients and MethodsWe report a 22‐year‐old male who presented with a large vascular malformation on his lower leg with both superficial capillary and deeper venous components, which on venography and ultrasound showed abnormal architecture and reflux into the superficial collecting system. He underwent a series of test areas with the PDL on the lower leg.ResultsUlceration developed in multiple sites after treatment as well as significant symptoms and signs of varicose eczema, which took at least 2 months to heal despite appropriate topical treatments and compression.ConclusionsPatients with vascular malformations of the lower leg with deeper venous components should be warned about the development of ulceration and treated with caution. Lasers Surg. Med. 32:396–398, 2003. © 2003 Wiley‐Liss, Inc.