bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic pain after colorectal cancer treatment: A population‐based cross‐sectional study
Authors: Elfeki Hossam , Alharbi Reem A. , Juul Therese , Drewes Asbjørn M. , Christensen Peter , Laurberg Søren , Emmertsen Katrine J. 
Published: January 2025

AbstractAimsWith the recently validated tool for estimating chronic pain after colorectal cancer surgery, the aims of this study were to calculate the prevalence and to identify predictive risk factors for chronic pain after colorectal cancer treatment.MethodClinical data from colorectal cancer patients treated between 2001 and 2014 were obtained from the Danish Colorectal Cancer Group database. In 2016, all survivors were invited to participate in a national cross‐sectional questionnaire study on long‐term functional outcomes, including the chronic pain questionnaire. The prevalence of chronic pain was analysed in groups based on demographic data and treatment‐related factors. Multivariate logistic regression analysis was performed to determine significant predictors of chronic pain, displayed as odds ratios (OR) and 95% confidence intervals.ResultsThe response rate was 63.8%, representing 11 600 patients (7127 colon and 4473 rectal cancer patients). The overall prevalence of pain was 41.5%, with 15.4% having major pain. Major pain was more prevalent in rectal cancer patients than colon cancer patients (16.7% vs. 14.5%, p < 0.001). Predictors for major pain were: young age <60 years [OR 1.7 (95% CI 1.51–1.91)]; female sex [OR 1.63 (95% CI 1.46–1.82)]; an open surgical approach [OR 1.18 (95% CI 1.05–1.33)]; chemotherapy [OR 1.32 (95% CI 1.18–1.49)]; radiotherapy [OR 1.39 (95% CI 1.17–1.65)]; permanent stoma [1.56 (95% CI 1.31–1.86)]; comorbidity [OR 1.69 (95% CI 1.5–1.9)]; anastomotic leakage [OR 1.31 (95% CI 1.05–1.64)].ConclusionChronic pain is prevalent after colorectal cancer surgery. All patients should be informed about the risks before cancer treatment, particularly those who are at an increased risk of developing major pain.
Hot child in the city: Drivers of urban buffelgrass presence in Tucson, Arizona
Authors: Hovanes Katherine A. , Gornish Elise S. , Thies Sawyer , Baldwin Elizabeth , Ossanna Lia , Dosamantes Elena , Lien Aaron 
Published: January 2025

AbstractInvasive species distributions and ecological impacts in natural ecosystems have been broadly studied, but invasive species urban distributions and impacts on human populations warrant further investigation. Urban areas are highly disturbed environments at high risk of invasion by non‐native species, and urban infrastructure can influence the dispersal and abundance of invasive species. Furthermore, in areas with concentrated human populations, invasive species may pose a risk to human as well as native biota. Here, we examine (1) how high‐traffic roadways and the presence of suitable habitat influence buffelgrass abundance in residential areas and (2) whether buffelgrass differentially invades residential areas across socioeconomic levels and racial diversity indices in Tucson, Arizona. We found that, within residential areas, the presence of vacant lots was positively associated with buffelgrass abundance; however, there was no relationship between other suitable habitat types and buffelgrass abundance. We found no relationship between road type and buffelgrass abundance in residential areas. We found that lower‐income communities were more likely to be invaded by buffelgrass, but there was no relationship between racial diversity index and buffelgrass abundance. Understanding drivers of invasive species presence and abundance in urban areas is necessary to inform urban management strategies to prevent spread to surrounding wildlands.
Identifying the Views and Needs of Family Physicians on Providing Care to Living Kidney Donors: A Cross‐Sectional Survey
Authors: Loban Katya , Trinh Emilie , Gaudio Kathleen , Nijjar Diya , Robert Jorane‐Tiana , Lam Ngan , McKay Scott , Badenoch Heather , Fortin Marie‐Chantal , Bugeja Ann , Mainra Rahul , Dipchand Christine , Sandal Shaifali 
Published: January 2025

ABSTRACTOptimizing the long‐term care and follow‐up of living kidney donors (LKDs) has been challenging, and prior LKDs have reported suboptimal healthcare experiences. Long‐term care of LKDs is largely undertaken by primary care practitioners such as family physicians (FPs). We conducted a cross‐sectional survey of Canadian FPs (n = 151). In our sample, 21.9% of participants reported that ≥1 patient had expressed interest in becoming a LKD, and 39.9% provided care to prior LKDs. While 55.5% knew how to find information on living kidney donation, 75.5% reported that information was not available in their practice. Only a minority had formal training in living kidney donation (<5%), and self‐reported knowledge was low (median = 3 [scale 1 = not strong to 10 = very strong]). Knowledge improved significantly with educational activities, resources, experience, and practice needs. Attitudes toward living kidney donation were generally favorable with 71.5% stating that FPs should be involved in post‐donation care. Clinical care guidelines (78.8%) were the most desired resource, followed by clear communication and reliable contact at transplant centers. Our findings inform the transplant community of an avenue to optimize LKD care by better‐supporting FPs, who provide care to LKDs. This may enhance data collection on LKD outcomes and potentially increase donation rates.
Evaluation of the Effect of Loperamide on the Cardiac Repolarization Interval Using Exposure–Response Analysis
Authors: Valenzuela Belén , Gisleskog Per Olsson , Cirillo Iolanda , Coenen Erwin , Ariyawansa Jay , Ali Saberi Rana , Takhtoukh Samiha , Pérez‐Ruixo Juan José , Ackaert Oliver 
Published: January 2025

ABSTRACTThis analysis assessed the relationship between the plasma concentrations of loperamide and its N‐desmethyl loperamide meta‐ bolite (M1) and the potential QT interval prolongation at therapeutic and supratherapeutic doses. The exposure–response analysis was performed using the data from healthy adults participating in a randomized, double‐blind, single‐dose, four‐way (placebo; loperamide 8 mg [therapeutic]; loperamide 48 mg [supratherapeutic]; moxifloxacin 400 mg [positive control]) crossover study. The electrocardiographic measurements extracted from 12‐lead digital Holter recordings were time‐matched to pharmacokinetic sampling of loperamide/M1. The primary response variable was placebo‐adjusted change from baseline in Fridericia‐corrected QT interval (ΔΔQTcF); the exposure variable was loperamide and/or M1 concentration. A total of 53 participants with 1408 time‐matched pharmacokinetic and ΔΔQTcF measurements was analyzed. Hysteresis between both loperamide and M1 concentrations and ΔΔQTcF was observed with supratherapeutic dose. The pre‐specified linear concentration‐ΔΔQTcF relationship was driven by M1 concentrations in the effect compartment. The model‐predicted mean ΔΔQTcF at the geometric mean of the maximum concentration in the effect compartment was −0.526 msec (90% CI, −1.51 to 0.462) following 8‐mg dose (2.1 ng/mL) and 6.06 msec (90% CI, 3.86–8.27) following 48‐mg dose (14.2 ng/mL). The upper bound of two‐sided 90% CI was < 10 msec for both doses. The sensitivity analysis considering loperamide concentrations in the effect compartment instead of M1 as input for the concentration‐ΔΔQTcF analysis confirmed these findings. The data showed that loperamide or M1 does not have an effect on cardiac repolarization that exceeds the threshold of regulatory concern in healthy participants at doses of 8 and 48 mg.
Sodium‐glucose cotransporter 2 inhibitors and outcomes in transthyretin amyloid cardiomyopathy: Systematic review and meta‐analysis
Authors: Karakasis Paschalis , Theofilis Panagiotis , Patoulias Dimitrios , Schuermans Art , Vlachakis Panayotis K , Klisic Aleksandra , Rizzo Manfredi , Fragakis Nikolaos 
Published: January 2025

AbstractBackgroundTransthyretin amyloid cardiomyopathy (ATTR‐CM) commonly leads to heart failure but has traditionally been an exclusion criterion in randomized clinical trials (RCTs) of sodium‐glucose cotransporter 2 inhibitors (SGLT2i); therefore, the effects of these drugs in this population remain undocumented. In light of recent studies, this meta‐analysis aimed to investigate the effect of SGLT2i on the prognosis of patients with ATTR‐CM.MethodsA comprehensive search of Medline, Scopus, and the Cochrane Library was conducted up to November 17, 2024. Study selection, data extraction and quality assessment were carried out independently by two investigators. Associations of SGLT2i with outcomes were pooled using random‐effects meta‐analyses.ResultsA total of five studies (9766 participants, 4 propensity score‐matched) were included. The use of SGLT2i was associated with significant reductions in all‐cause mortality [hazard ratio (HR) .54, 95% confidence interval (CI) .44–.66], cardiovascular mortality (HR .39, 95% CI .23–.65), major adverse cardiovascular events (HR .71, 95% CI .61–.83), and heart failure hospitalizations (HFHs) (HR .63, 95% CI .52–.77) compared to non‐use. The odds of cardiac arrhythmias were significantly lower among SGLT2i users compared to non‐users [odds ratio (OR) .73, 95% CI .65–.83]. Specifically, SGLT2i use was associated with significant reductions in the odds of atrial fibrillation (AF) (OR .75, 95% CI .62–.91), ventricular tachycardia (OR .72, 95% CI .59–.88), and sudden cardiac arrest (OR .71, 95% CI .50–.99).ConclusionsThe use of SGLT2is may be associated with a more favourable prognosis in patients with ATTR‐CM. Adequately powered, long‐term RCTs are required to validate the available observational evidence.
Responsible Use of Population Neuroscience Data in the ABCD: Towards Standards of Accountability and Integrity
Authors: Brown Sandra A. , Garavan Hugh , Jernigan Terry L. , Tapert Susan F. , Huber Rebekah S. , Lopez Daniel , Murray Traci , Dowling Gayathri , Hoffman Elizabeth A. , Uddin Lucina Q. 
Published: January 2025

ABSTRACTThis editorial focuses on the issue of data misuse that is increasingly evidenced in social media as well as some premiere scientific journals. This issue is of critical importance to open science projects in general, and ABCD in particular, given the broad array of biological, behavioural and environmental information collected on this American sample of 12,000 youth and parents. ABCD data are already widely used with over 1,200 publications and twice as many citations per year as expected (relative citation index based on year, field and journal). However, the adverse consequences of misuse of data and inaccurate interpretation of emergent findings from this precedent setting study may have a profound impact on disadvantaged populations and perpetuate biases and societal injustices.
Bridging the work governance divide: Pluralism and performance
Authors: de Oliveira Gustavo Magalhães , Saes Maria Sylvia Macchione , de Amorim Wilson Aparecido Costa , Grandori Anna 
Published: January 2025

AbstractThis article introduces a new direction of studies that looks at the Workplace of the Future through enlarged interdisciplinary lenses. This article bridges the divide between different traditions – human resource management, industrial relations and economic democracy – arguing theoretically and demonstrating empirically their complementarity in designing a workplace that better responds to the challenges posed by increasingly conflicting demands, such as flexibility and safeguards; quality of life and quality of productive output; diversity of workforce; and diversity of contexts and institutions. We hypothesise and find (using the largest available dataset on work‐related systems CRANET) that the response may lie in adopting ‘pluralist’ work governance models, blending mechanisms that differ in kind and are recommended by different disciplinary traditions: ‘highly powered incentives’, worker representation and voice rights, communitarian practices and formal personnel systems. We also show that pluralist systems in the ‘private governance’ sphere are complementary with strong labour‐related institutions in the ‘public governance’ of work; and indicate how those findings can lead to a better understanding of how to take into account institutions, such as governments, regulations and industries, in shaping and designing the workplace of the future. In particular, the finding that a pluralist configuration consistently outperforms other configurations across industries and countries suggests that a pluralist trait may be a universally valid attribute of workplace governance, even though the specific mechanisms that create that variety are different and context‐dependent.
A Recursive Model Approach to Include Epigenetic Effects in Genetic Evaluations Using Simulated DNA Methylation Effects
Authors: López‐Catalina Adrián , Ragab Mohamed , Reverter Antonio , González‐Recio Oscar 
Published: January 2025

ABSTRACTThe advancement of epigenetics has highlighted DNA methylation as an intermediate‐omic influencing gene regulation and phenotypic expression. With emerging technologies enabling the large‐scale and affordable capture of methylation data, there is growing interest in integrating this information into genetic evaluation models for animal breeding. This study used methylome information from six dairy cows to simulate the methylation profile of 13,183 genotyped animals. The liability to methylation was treated as an additive trait, while a trait moderated by methylation effects was also simulated. A multiomic model (GOBLUP) was adapted to incorporate methylation data in genomic and genetic evaluations, using the traditional BLUP method as a benchmark. The GOBLUP accurately recovered heritability estimates for the liability to methylation in all low, medium and high heritability scenarios and was consistent at estimating the heritability for the epigenetics‐moderated trait of interest at a low–medium heritability of 0.14. The genetic variance recovered by the BLUP model was influenced by the h2 of the liability to methylation, and a part of the methylation variance for the phenotypic trait was captured as additive. The h2 of the phenotypic trait partially relies on the h2 value for the methylation windows in the traditional model. A newly proposed estimated epigenetic value (EEV) combines the traditional additive genetic information from genotyping arrays with epigenetic information. The correlation between the traditional estimated breeding value (EBV) and EEV was high (0.92–0.99 depending on the scenario), but the correlation of the EEV with the true breeding value was higher than the correlation between the traditional EBV and the TBV (0.85 vs. 0.75, 0.71 vs. 0.66 and 0.61 vs. 0.62 depending on the scenario). This study demonstrates that the GOBLUP multiomic recursive model can effectively separates additive and epigenetic variances, enabling improved breeding decisions by accounting for genetic liability to DNA methylation. This enables more informed breeding decisions, optimising selection for desired traits. Emerging sequencing techniques offer new opportunities for cost‐effective simultaneous acquisition of genetic and epigenetic data, further enhancing breeding accuracy.
Integrative Machine Learning of Glioma and Coronary Artery Disease Reveals Key Tumour Immunological Links
Authors: He Youfu , You Ganhua , Zhou Yu , Ai Liqiong , Liu Wei , Meng Xuantong , Wu Qiang 
Published: January 2025

ABSTRACTIt is critical to appreciate the role of the tumour‐associated microenvironment (TME) in developing strategies for the effective therapy of cancer, as it is an important factor that determines the evolution and treatment response of tumours. This work combines machine learning and single‐cell RNA sequencing (scRNA‐seq) to explore the glioma tumour microenvironment's TME. With the help of genome‐wide association studies (GWAS) and Mendelian randomization (MR), we found genetic variants associated with TME elements that affect cancer and cardiovascular disease outcomes. Using machine learning techniques high dimensional data was analysed to obtain new molecular sub‐types and biomarkers that are important for prognosis and treatment response. F3 was identified as a top regulator and revealed potential angiogenic and immunogenic characteristics within the TME that could be harnessed in immunotherapy. These results demonstrate the potential of machine‐learning approaches in identifying and dissecting TME heterogeneity and informing treatment in precision oncology. This work proposes improving the immunotherapeutic response through targeted modulation of relevant cellular and molecular interactions.
The effect of exercise on GDF‐15 levels in individuals with prediabetes: A randomized controlled trial
Authors: Yıldırım Ayaz Elif , Mesci Banu , Üner Özden Ezgi , Kaya Fatoş Nimet , Dincer Berna , İşman Ferruh Kemal , Oğuz Aytekin 
Published: January 2025

ABSTRACTAimsGrowth differentiation factor‐15 (GDF‐15) is an inflammatory cytokine that increases in prediabetes and is known for its anorexigenic effects. This study aims to evaluate the effects of a 12‐week exercise program on GDF‐15 in individuals with prediabetes.Materials and MethodsIn this multicenter, parallel‐group, randomized‐controlled trial, 64 patients aged 18–60 diagnosed with prediabetes were randomized in a 1:1 ratio into the exercise group (E) and the control group (C). Additionally, 32 patients who were planned to start metformin were included in the metformin group (M). Participants in the exercise group engaged in aerobic exercise at 50–70% of their maximum heart rate for 60 min, 3 days a week. Serum GDF‐15 levels were evaluated at the beginning and the end of the 12th week.ResultsThe mean age of the 91 participants who completed the study was 46.13 ± 8.52 years, and 23.1% were male. Basal GDF‐15 levels were similar among the groups (E = 668.6 ± 415.1, C = 651.8 ± 352.5, M = 603.6 ± 387.2, P = 0.47). At the 12th week, GDF‐15 levels were lower in the E compared to the C, while higher in the M compared to the C (E = 383.1 ± 215.6, C = 556.4 ± 285.6, M = 810.8 ± 498.0, P < 0.001). In inter‐group comparisons, no significant change was observed in the C between the 0th and 12th weeks, while GDF‐15 decreased in the E (P < 0.001) and increased in the M (P < 0.001).ConclusionsIt was determined that in individuals with prediabetes, GDF‐15, which serves both as a biomarker of metabolic disorder and has a negative regulatory effect on appetite, decreased with 12 weeks of aerobic exercise and increased with metformin administration.
Characterization of Allobodo yubaba sp. nov. and Novijibodo darinka gen. et sp. nov., cultivable free‐living species of the phylogenetically enigmatic kinetoplastid taxon Allobodonidae
Authors: Packer Julia A. , Zavadska Daryna , Weston Elizabeth J. , Eglit Yana , Richter Daniel J. , Simpson Alastair G. B. 
Published: January 2025

AbstractKinetoplastids are a large and diverse protist group, spanning ecologically important free‐living forms to medically important parasites. The taxon Allobodonidae holds an unresolved position within kinetoplastids, and the sole described species, Allobodo chlorophagus, is uncultivated, being a necrotroph/parasite of macroalgae. Here we describe Allobodo yubaba sp. nov. and Novijibodo darinka gen. nov. et sp. nov., both free‐living bacterivores isolated into monoeukaryotic cultures. Electron microscopy shows that both A. yubaba and N. darinka have a microtubular prism in the feeding apparatus (absent in A. chlorophagus), and an ovoid eukinetoplast, rather than pan‐kDNA as in A. chlorophagus. Phylogenetic analyses of SSU rDNA sequences robustly place A. yubaba as the sister to A. chlorophagus, while N. darinka branches separately within Allobodonidae, as a sister group of undescribed freshwater isolates. We view Allobodonidae as containing at least four genus‐level clades: Allobodo (A. chlorophagus and A. yubaba n. sp.), an undescribed fresh‐water clade, an undescribed marine clade, and now Novijibodo—with N. darinka as its sole known member. Electron microscopy also revealed a rod‐shaped gram‐negative bacterial cytoplasmic endosymbiont in our N. darinka isolate. The availability of these species in monoeukaryotic culture should facilitate future research, including resolving the position of Allobodonidae using phylogenomic approaches.
Ultrastructure of Olkasia polycarbonata (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses
Authors: Palka Maia V. , Manglicmot Regine Claire , Lax Gordon , Wakeman Kevin C. , Leander Brian S. 
Published: January 2025

AbstractEuglenids are flagellates with diverse modes of nutrition, including the photosynthetic Euglenophyceae, which acquired plastids via secondary endosymbiosis with green algae, and a diverse assemblage of predators of bacteria and other microeukaryotes. Most heterotrophic euglenids have never been cultivated, so their morphology remains poorly characterized and limited to only a few studies. “Ploeotids” are a paraphyletic group representing much of the diversity of heterotrophic euglenids and are characterized by their feeding apparatus and a rigid pellicle of 10–12 longitudinally arranged strips. Ploeotid‐like euglenids gave rise to the Spirocuta, a large clade of heterotrophic and photosynthetic euglenids defined by a flexible pellicle of helically arranged strips. Using single‐cell approaches, we report the first ultrastructural characterization of Olkasia polycarbonata, a ploeotid that is consistently positioned as the sister lineage to the Spirocuta in multigene phylogenetic analyses. O. polycarbonata shares several morphological characteristics with members of Spirocuta, such as prominent swellings on the paraxonemal rods and a robust feeding apparatus consisting of rods and vanes. These morphological traits are consistent with the phylogenetic position of O. polycarbonata and demonstrate an increase in cytoskeletal complexity that occurred prior to the key strip duplication event in the most recent common ancestor of Spirocuta.
A deep dive into bryophyte genome space reveals opposing evolutionary trends in the sister lineages, mosses and liverworts
Authors: Fujiwara Tao , Liu Hong‐Mei , Zhu Rui‐Liang , Schneider Harald 
Published: January 2025

AbstractAmong the lineages of the tree of life, land plants exhibit a remarkably high genomic disparity because of their distinct evolutionary trajectories in the phylogenetic history of their major lineages. The macroevolutionary pattern of genomic evolution has been mainly investigated to obtain insights into well‐studied lineages such as angiosperms, but little attention has been given to many important lineages such as bryophytes. This study was designed to resolve this gap by comparing the genomic evolution trajectories of mosses and liverworts. Thus, a data set comprising chromosome number and genome size was compiled, including previously published and newly generated data that were used to trace the phylogenetic history of these two parameters among mosses and liverworts via ancestral state reconstruction and phylogenetic comparative analyses. Contrasting patterns of chromosome number and genome size evolutions were detected between the two sister lineages. Mosses accumulated high chromosome number disparity via repeated whole‐genome duplications and descending dysploidy but maintained a small genome size. By contrast, the chromosome number of liverworts was highly conserved, and heterogeneous trends in genome size evolution were identified among major lineages. These contrasting patterns may be partly explained by the difference in genomic dynamics: Active dynamics enables genome downsizing and reorganization in mosses, whereas genome stability leads to the accumulation of large genomes in liverworts. The results of this study confirmed the distinct trends of genomic evolution in bryophytes.