bluuebunny/crossref_metadata_2025_split huggingface dataset:
The long-term impacts of opioid use before and after joint arthroplasty: matched cohort analysis of New Zealand linked register data
Authors: Pryymachenko Yana , Wilson Ross , Abbott John Haxby , Dowsey Michelle , Choong Peter 
Published: December 2023

Abstract

Background
Opioids are commonly used both before and after total joint arthroplasty (TJA).

Objective
The objective of this study was to estimate the long-term effects of pre- and perioperative opioid use in patients undergoing TJA.

Methods
We used linked population datasets to identify all (n =18,666) patients who had a publicly funded TJA in New Zealand between 2011 and 2013. We used propensity score matching to match individuals who used opioids either before surgery, during hospital stay, or immediately post-discharge with individuals who did not based on a comprehensive set of covariates. Regression analysis was used to estimate the effect of opioid use on health and socio-economic outcomes over 5 years.

Results
Opioid use in the 3 months prior to surgery was associated with significant increases in healthcare utilization and costs (number of hospitalizations 6%, days spent in hospital 14.4%, opioid scripts dispensed 181%, and total healthcare costs 11%). Also increased were the rate of receiving social benefits (2 percentage points) and the rates of opioid overdose (0.5 percentage points) and mortality (3 percentage points). Opioid use during hospital stay or post-discharge was associated with increased long-term opioid use, but there was little evidence of other adverse effects.

Conclusions
Opioid use before TJA is associated with significant negative health and economic consequences and should be limited. This has implications for opioid prescribing in primary care. There is little evidence that peri- or post-operative opioid use is associated with significant long-term detriments.
Neuromuscular Dysfunction Precedes Cognitive Impairment in a Mouse Model of Alzheimer’s Disease
Authors: Brisendine Matthew H , Nichenko Anna S , Bandara Aloka B , Willoughby Orion S , Amiri Niloufar , Weingrad Zach , Specht Kalyn S , Bond Jacob M , Addington Adele , Jones Ronald G , Murach Kevin A , Poelzing Steven , Craige Siobhan M , Grange Robert W , Drake Joshua C 
Published: December 2023

Abstract
Alzheimer’s disease (AD) develops along a continuum that spans years prior to diagnosis. Decreased muscle function and mitochondrial respiration occur years earlier in those that develop AD; however, it is unknown what causes these peripheral phenotypes in a disease of the brain. Exercise promotes muscle, mitochondria, and cognitive health and is proposed to be a potential therapeutic for AD, but no study has investigated how skeletal muscle adapts to exercise training in an AD-like context. Utilizing 5xFAD mice, an AD model that develops ad-like pathology and cognitive impairments around 6 mo of age, we examined in vivo neuromuscular function and exercise adapations (mitochondrial respiration and RNA sequencing) before the manifestation of overt cognitive impairment. We found 5xFAD mice develop neuromuscular dysfunction beginning as early as 4 mo of age, characterized by impaired nerve-stimulated muscle torque production and compound nerve action potential of the sciatic nerve. Furthermore, skeletal muscle in 5xFAD mice had altered, sex-dependent, adaptive responses (mitochondrial respiration and gene expression) to exercise training in the absence of overt cognitive impairment. Changes in peripheral systems, specifically neural communication to skeletal muscle, may be harbingers for AD and have implications for lifestyle interventions, like exercise, in AD.
Expression profiles of oviductal mRNAs and lncRNAs in the follicular phase and luteal phase of sheep (Ovis aries) with 2 fecundity gene (FecB) genotypes
Authors: Chen Weihao , Li Zhifeng , Zhong Rongzhen , Sun Wei , Chu Mingxing 
Published: December 2023

Abstract
FecB (also known as BMPR1B) is a crucial gene in sheep reproduction, which has a mutation (A746G) that was found to increase the ovulation rate and litter size. The FecB mutation is associated with reproductive endocrinology, such mutation can control external estrous characteristics and affect follicle-stimulating hormone during the estrous cycle. Previous researches showed that the FecB mutation can regulate the transcriptomic profiles in the reproductive-related tissues including hypothalamus, pituitary, and ovary during the estrous cycle of small-tailed Han (STH) sheep. However, little research has been reported on the correlation between FecB mutation and the estrous cycle in STH sheep oviduct. To investigate the coding and noncoding transcriptomic profiles involved in the estrous cycle and FecB in the sheep oviduct, RNA sequencing was performed to analyze the transcriptomic profiles of mRNAs and long noncoding RNAs (lncRNAs) in the oviduct during the estrous cycle of STH sheep with mutant (FecBBB) and wild-type (FecB++) genotypes. In total, 21,863 lncRNAs and 43,674 mRNAs were screened, the results showed that mRNAs had significantly higher expression levels than the lncRNAs, and the expression levels of these screened transcripts were lower in the follicular phase than they were in the luteal phase. Among them, the oviductal glycoprotein gene (OVGP1) had the highest expression level. In the comparison between the follicular and luteal phases, 57 differentially expressed (DE) lncRNAs and 637 DE mRNAs were detected, including FSTL5 mRNA and LNC_016628 lncRNA. In the comparison between the FecBBB and FecB++ genotypes, 26 DE lncRNAs and 421 DE mRNAs were detected, including EEF1D mRNA and LNC_006270 lncRNA. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis indicated that the DE mRNAs were enriched mainly in terms related to reproduction such as the tight junction, SAGA complex, ATP-binding cassette, nestin, and Hippo signaling pathway. The interaction network between DE lncRNAs and DE mRNAs indicated that LNC_018420 may be the key regulator in sheep oviduct. Together, our results can provide novel insights into the oviductal transcriptomic function against a FecB mutation background in sheep reproduction.
New insights into the (epi)genetics of twinning
Authors: van Dongen Jenny , Hubers Nikki , Boomsma Dorret I 
Published: December 2023

AbstractSpontaneous dizygotic (DZ) twins, i.e. twins conceived without the use of ARTs, run in families and their prevalence varies widely around the globe. In contrast, monozygotic (MZ) twins occur at a constant rate across time and geographical regions and, with some rare exceptions, do not cluster in families. The leading hypothesis for MZ twins, which arise when a zygote splits during preimplantation stages of development, is random occurrence. We have found the first series of genes underlying the liability of being the mother of DZ twins and have shown that being an MZ twin is strongly associated with a stable DNA methylation signature in child and adult somatic tissues. Because identical twins keep this molecular signature across the lifespan, this discovery opens up completely new possibilities for the retrospective diagnosis of whether a person is an MZ twin whose co-twin may have vanished in the early stages of pregnancy. Here, we summarize the gene finding results for mothers of DZ twins based on genetic association studies followed by meta-analysis, and further present the striking epigenetic results for MZ twins.
Prevalence and determinants of post-acute sequelae of COVID-19 in Liberia
Authors: Gwaikolo Cozie , Sackie-Wapoe Yatta , Badio Moses , Glidden David V , Lindan Christina , Martin Jeffrey 
Published: December 2023

Abstract

Background
Evidence from resource-rich settings indicates that many people continue to have persistent symptoms following acute SARS-CoV-2 infection, called post-acute sequelae of COVID-19 (PASC). Only a few studies have described PASC in sub-Saharan Africa (SSA). We aimed to describe PASC in Liberia.

Methods
We randomly sampled all people who were reported from the most populous county to the Liberian Ministry of Health (MOH) as having a laboratory-confirmed SARS-CoV-2 infection from June to August 2021. We interviewed individuals by phone 3 to 6 months later. Those with persistence of at least one symptom were considered to have PASC.

Results
From among 2848 people reported to the MOH from Montserrado County during the period of interest, we randomly selected 650; of these, 548 (84.3%) were reached and 505 (92.2%) of those who were contacted were interviewed. The median age was 38 years (interquartile range (IQR), 30–49), and 43.6% were female. During acute infection, 40.2% were asymptomatic, 53.9% had mild/moderate disease and 6.9% had severe/critical disease. Among the 59.8% (n = 302) who were initially symptomatic, 50.2% (n = 152) reported at least one persistent symptom; the most common persistent symptoms were fatigue (21.2%), headache (16.2%) and cough (12.6%); 40.1% reported that PASC significantly affected their daily activities. Being hospitalized with moderate disease [adjusted prevalence ratio (aPR), 2.00 (95% CI, 1.59 to 2.80] or severe/critical disease [aPR, 2.11 (95% CI, 1.59 to 2.80)] was associated with PASC, compared with those not hospitalized. Females were more likely than males to report persistent fatigue [aPR, 1.67 (95% CI, 1.08 to 2.57)].

Conclusions
Our findings suggest that persistent symptoms may have affected a large proportion of people with initially symptomatic COVID-19 in west Africa and highlight the need to create awareness among infected people and health care professionals.
Embracing the Brave New World: A Response to Demekas and Grippa
Authors: Gruenewald Seraina , Knijp Gerdie , Schoenmaker Dirk , van Tilburg Rens 
Published: December 2023

ABSTRACT
In the article ‘Walking a Tightrope: Financial Regulation, Climate Change, and the Transition to a Low-Carbon Economy’ in the Journal of Financial Regulation, Demekas and Grippa (2022) argue that it is dangerous to give central banks and financial regulators a ‘promotional’ role in the transition to a low-carbon economy.
In this article we argue that both climate change and the transition to a low-carbon economy are clear and present dangers for financial stability. Accelerating the transition through proper risk management, both of micro and macro risks, should therefore be what regulators should do. Regulators should accept that they need to act with incomplete information. We agree with Demekas and Grippa that financial supervisors should not be the only, or even first, game in town. Nevertheless, we disagree that the effect of their action will be negligible. Financial supervisors should embrace the brave new world and play their role in addressing climate and environmental risks within the financial system.
Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland
Authors: Wang Ziqi , Wang Jie , Wu Honghui , Yang Tian , An Yixin , Zhang Yunlong , Bian Jianlin , Li Ying , Ren Haiyan , Lkhagva Ariuntsetseg , Wang Xu , Yu Qiang 
Published: December 2023

Abstract
Nitrogen (N) deposition exhibits significant impacts on ecosystem functions and processes. Previous studies have indicated that N addition has an impact on the stoichiometry of plant leaf C:N:P ratios. However, few studies have focused on effects of N addition on belowground systems. This study aims to examine the impact of 7 years of N addition on above- and belowground C:N:P stoichiometry at plant community level in a temperate grassland located in Inner Mongolia. A 7-year field N addition experiment was conducted, which included six treatments: Cont: control; N1: 0.4 mol·m−2 N; N2: 0.8 mol·m−2 N; N3: 1.6 mol·m−2 N; N4: 2.8 mol·m−2 N; N5: 4 mol·m−2 N with six replicates. Above- and belowground plant biomass and C:N:P stoichiometry were measured and analyzed. Our results showed that N addition resulted in a reduction of aboveground C concentration, but an increase in aboveground N and P concentrations, with a decrease in C:N and C:P ratios and an increase in N:P ratio. Furthermore, the aboveground C, N, and P pools all exhibited an increase as a result of N addition. However, N addition did not have any significant effect on belowground C, N, P concentrations, ratios, pools, or stoichiometric characteristics in the soil layers of 0–10, 10–30, 30–50, and 50–100 cm. These results suggest that increasing levels of N deposition significantly alter the aboveground C:N:P stoichiometry at the plant community level, which may affect functions and processes in the grassland ecosystem, but have little effect on belowground C:N:P stoichiometry.
Effects of the combination of Lactobacillus helveticus and isomalto-oligosaccharide on survival, gut microbiota, and immune function in Apis cerana worker bees
Authors: Fang Pingping , Lei Qingzhi , Lv Mingkui , Xu Le , Dong Kun , Zhao Wenzheng , Yue Dan , Cao Zhenhui , Lin Qiuye 
Published: December 2023

Abstract
The adult worker bees were fed sucrose syrup or sucrose syrup supplemented with Lactobacillus helveticus KM7, prebiotic isomalto-oligosaccharide (IMO), or L. helveticus KM7 combined with IMO. Survival rate, gut microbiota, and gene expression of gut antimicrobial peptides in worker honey bees were determined. Administration of L. helveticus KM7 and IMO significantly increased the survival rate in worker bees relative to bees fed sucrose only. Then, higher concentration of both lactic acid bacteria and Bifidobacterium in the gut and lower counts of gut fungi, Enterococcus, and Bacteroides–Porphyromonas–Prevotella were observed in bees fed the combination of KM7 and IMO compared with control bees. The combination of L. helveticus KM7 with IMO showed a greater or comparable modulating effect on those bacteria relative to either KM7 or IMO alone. Furthermore, the combination treatment of L. helveticus KM7 and IMO enhanced mRNA expression of antimicrobial peptide genes, including Abaecin, Defensin, and the gene encoding prophenoloxidase (PPO) in the gut compared with both control bees and those either L. helveticus KM7 or IMO alone. These results suggest that the combination of L. helveticus KM7 and IMO synergistically modifies the gut microbiota and immunity and consequently improves the survival rate of Apis cerana adult workers.
Scanning for dark matter subhaloes in Hubble Space Telescope imaging of 54 strong lenses
Authors: Nightingale James W , He Qiuhan , Cao Xiaoyue , Amvrosiadis Aristeidis , Etherington Amy , Frenk Carlos S , Hayes Richard G , Robertson Andrew , Cole Shaun , Lange Samuel , Li Ran , Massey Richard 
Published: December 2023

ABSTRACT
The cold dark matter (DM) model predicts that every galaxy contains thousands of DM subhaloes; almost all other DM models include a physical process that smooths away the subhaloes. The subhaloes are invisible, but could be detected via strong gravitational lensing, if they lie on the line of sight to a multiply imaged background source, and perturb its apparent shape. We present a predominantly automated strong lens analysis framework, and scan for DM subhaloes in Hubble Space Telescope imaging of 54 strong lenses. We identify five DM subhalo candidates, including two especially compelling candidates (one previously known in SLACS0946 + 1006) where a subhalo is favoured after all of our tests for systematics. We find that the detectability of subhaloes depends upon the assumed parametric form for the lens galaxy’s mass distribution, especially its degree of azimuthal freedom. Using separate components for DM and stellar mass reveals two DM subhalo candidates and removes four false positives compared to the single power-law mass model that is common in the literature. We identify 45 lenses without substructures, the number of which is key to statistical tests able to rule out models of, for example, warm or self-interacting DM. Our full analysis results are available at https://github.com/Jammy2211/autolens_subhalo.
Orbit decay in encounters between anisotropic spherical galaxies of equal mass
Authors: Saleh Lucas , Barnes Joshua E 
Published: December 2023

ABSTRACT
We investigate the effect of radial anisotropy on the rate of orbit decay in parabolic encounters of identical spherical galaxies. Our galaxy models have Hernquist density profiles and Osipkov–Merritt velocity distributions. We find that radially anisotropic models merge in as little as half the time of their isotropic counterparts. Anisotropic models are more susceptible to tidal deformation; this accelerates the transfer of orbital angular momentum to internal degrees of freedom. Even during the initial approach, the anisotropic models become more distorted, and arrive at pericentre already having lost substantial amounts of angular momentum. Our results may have implications for estimates of merger rates and the persistence of tidal tails.
MUSE observations of the optical nebula surrounding the central compact object in the Vela Junior supernova remnant
Authors: Suherli Janette , Safi-Harb Samar , Seitenzahl Ivo R , Ghavamian Parviz , Ho Wynn C G , Li Chuan-Jui , Ruiter Ashley J , Sutherland Ralph S , Vogt Frédéric P A 
Published: December 2023

ABSTRACT
Central compact objects (CCOs), neutron stars found near the centre of some supernova remnants (SNRs), have been almost exclusively studied in X-rays and are thought to lack the wind nebulae typically seen around young, rotation-powered pulsars. We present the first, spatially resolved, morphological and spectroscopic study of the optical nebula observed at the location of CXOU J085201.4−461753, the CCO in the heart of the Vela Junior SNR. It is currently the only Galactic CCO with a spatially coincident nebula detected at optical wavelengths, whose exact nature remains uncertain. New Multi Unit Spectroscopic Explorer integral field spectroscopy data confirm that the nebula, shaped like a smooth blob extending 8 arcsec in diameter, is dominated by [N ii]${\lambda } {\lambda }$6548, 6583 emission. The data reveal a distinct and previously unobserved morphology of the H ${\alpha }$ emission, exhibiting an arc-like shape reminiscent of a bow shock nebula. We observe a significantly strong [N ii] emission relative to H ${\alpha }$, with the [N ii]${\lambda } {\lambda }$6548, 6583 up to 34 times the intensity of the H ${\alpha }$ emission within the optical nebula environment. Notably, the [N ii] and H ${\alpha }$ structures are not spatially coincident, with the [N ii] nebula concentrated to the south of the CCO and delimited by the H ${\alpha }$ arc-like structure. We detect additional emission in [N i], He i, [S ii], [Ar iii], [Fe ii], and [S iii]. We discuss our findings in the light of a photoionization or Wolf–Rayet nebula, pointing to a very massive progenitor and further suggesting that very massive stars do not necessarily make black holes.
Democratic Despotism, Democratic Culture, and the Democratic Ideal
Authors: Gooding-Williams Robert 
Published: December 2023

Abstract
In this paper, I outline Du Bois’s WWI-era theory of democracy, which comprises three parts: first, an historically specific explanation of the racially exclusionist character of the modern struggle for democracy; second, a justification of universal suffrage; and third, an account of democratic culture, the promotion of which he believed was necessary to supplement the enfranchisement of black people where white supremacy still threated the achievement of justice.
Contribution of tRNA sequence and modifications to the decoding preferences of E. coli and M. mycoides tRNAGlyUCC for synonymous glycine codons
Authors: Kompatscher Maria , Bartosik Karolina , Erharter Kevin , Plangger Raphael , Juen Fabian Sebastian , Kreutz Christoph , Micura Ronald , Westhof Eric , Erlacher Matthias D 
Published: December 2023

Abstract
tRNA superwobbling, used by certain bacteria and organelles, is an intriguing decoding concept in which a single tRNA isoacceptor is used to decode all synonymous codons of a four-fold degenerate codon box. While Escherichia coli relies on three tRNAGly isoacceptors to decode the four glycine codons (GGN), Mycoplasma mycoides requires only a single tRNAGly. Both organisms express tRNAGly with the anticodon UCC, which are remarkably similar in sequence but different in their decoding ability. By systematically introducing mutations and altering the number and type of tRNA modifications using chemically synthesized tRNAs, we elucidated the contribution of individual nucleotides and chemical groups to decoding by the E. coli and M. mycoides tRNAGly. The tRNA sequence was identified as the key factor for superwobbling, revealing the T-arm sequence as a novel pivotal element. In addition, the presence of tRNA modifications, although not essential for providing superwobbling, was shown to delicately fine-tune and balance the decoding of synonymous codons. This emphasizes that the tRNA sequence and its modifications together form an intricate system of high complexity that is indispensable for accurate and efficient decoding.
The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro
Authors: Wicke Dennis , Neumann Piotr , Gößringer Markus , Chernev Aleksandar , Davydov Swetlana , Poehlein Anja , Daniel Rolf , Urlaub Henning , Hartmann Roland K , Ficner Ralf , Stülke Jörg 
Published: December 2023

Abstract
Even though Bacillus subtilis is one of the most studied organisms, no function has been identified for about 20% of its proteins. Among these unknown proteins are several RNA- and ribosome-binding proteins suggesting that they exert functions in cellular information processing. In this work, we have investigated the RNA-binding protein YlxR. This protein is widely conserved in bacteria and strongly constitutively expressed in B. subtilis suggesting an important function. We have identified the RNA subunit of the essential RNase P as the binding partner of YlxR. The main activity of RNase P is the processing of 5′ ends of pre-tRNAs. In vitro processing assays demonstrated that the presence of YlxR results in reduced RNase P activity. Chemical cross-linking studies followed by in silico docking analysis and experiments with site-directed mutant proteins suggest that YlxR binds to the region of the RNase P RNA that is important for binding and cleavage of the pre-tRNA substrate. We conclude that the YlxR protein is a novel interaction partner of the RNA subunit of RNase P that serves to finetune RNase P activity to ensure appropriate amounts of mature tRNAs for translation. We rename the YlxR protein RnpM for RNase P modulator.
Positive and negative information effects on consumer preferences for lab grown meat
Authors: Kovacs Kent F , Kemper Nathan , Nayga Rodolfo M , Yang Wei , Blumenberg Aryn 
Published: December 2023

Abstract
We examine the effect of information framing on consumers’ preferences for In-vitro (or lab grown) meat (IVM). Our choice experiment uses eight choice tasks that vary across five attributes: production method (IVM or conventional), carbon trust label, organic label, animal welfare label, and price. We investigate four information treatments: (1) neutral (baseline), (2) positive, (3) negative, and (4) both positive and negative combined. Negative information framing leads consumers to require the largest discount to accept IVM, while positive information significantly reduces the discount required. Without positive information, food retailers should expect to offer steep discounts to attract customers to IVM.