bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoangiogenesis markers in canine urothelial carcinomas: A cross‐sectional study
Authors: Gentilini Fabio , Salaroli Roberta , Tornago Raimondo , Turba Maria Elena , Ogundipe Tolulope Grace , Zannoni Augusta , Forni Monica , Campigli Michela , Furlanello Tommaso 
Published: January 2024

AbstractBackgroundIn humans, there is a growing body of evidence that neoangiogenesis is crucial for tumour growth and progression in urothelial carcinomas (UC) which also typically exhibit overactivation of the RAS‐MAPK pathway. In canine UC (cUC), the same pathway has been aberrantly activated due to V595E BRAF variant and BRAF inhibitors has been evaluated as more effective treatment. However, BRAF inhibition is hampered in humans by rapidly occurring of chemoresistance. Targeting angiogenesis has been speculated to increase the effectiveness of BRAF inhibitors and to delay the development of chemoresistance.ObjectivesThis study aimed to investigate the level of angiogenic markers in urine samples of UC affected dogs (n = 15) in comparison to an unmatched control group (n = 16) along with the clinical, morphological and molecular features.MethodsIn urine, both vascular endothelial growth factor (VEGF) concentration, using an ELISA assay, and MMP2 and 9 activities, using the zymographic assay, were measured. BRAF analysis was carried out using a digital PCR method.ResultsUrinary VEGF concentration (mean pg/g_uCrea 6.9 ± 27.7 vs. 1074 ± 1797, p < 0.01) and MMP activity (mean 6.8 × 106 ± 9.2 × 106 vs. 2.5 × 107 ± 2.3 × 107, p < 0.05) were higher in affected dogs than in healthy controls. Urinary active MMP9 was significantly correlated with T3 stage, it was absent in dogs with undetectable VEGF and it correlated well with urinary VEGF concentration. In this cohort, 10/10 UC affected dogs exhibited the V595E BRAF variation.ConclusionThe findings are consistent with the presence of overactive neoangiogenesis in cUC. Urinary active MMP9 may be suitable for use as tumour progression biomarker. The addition of angiogenesis targeting may be rationale for novel therapeutic strategies.
MuSCs and IPCs: roles in skeletal muscle homeostasis, aging and injury
Authors: Jiang Haiyan , Liu Boya , Lin Junfei , Xue Tong , Han Yimin , Lu Chunfeng , Zhou Songlin , Gu Yun , Xu Feng , Shen Yuntian , Xu Lingchi , Sun Hualin 
Published: January 2024

AbstractSkeletal muscle is a highly specialized tissue composed of myofibres that performs crucial functions in movement and metabolism. In response to external stimuli and injuries, a range of stem/progenitor cells, with muscle stem cells or satellite cells (MuSCs) being the predominant cell type, are rapidly activated to repair and regenerate skeletal muscle within weeks. Under normal conditions, MuSCs remain in a quiescent state, but become proliferative and differentiate into new myofibres in response to injury. In addition to MuSCs, some interstitial progenitor cells (IPCs) such as fibro-adipogenic progenitors (FAPs), pericytes, interstitial stem cells expressing PW1 and negative for Pax7 (PICs), muscle side population cells (SPCs), CD133-positive cells and Twist2-positive cells have been identified as playing direct or indirect roles in regenerating muscle tissue. Here, we highlight the heterogeneity, molecular markers, and functional properties of these interstitial progenitor cells, and explore the role of muscle stem/progenitor cells in skeletal muscle homeostasis, aging, and muscle-related diseases. This review provides critical insights for future stem cell therapies aimed at treating muscle-related diseases.
Quantum Energy Inequalities in Integrable Models with Several Particle Species and Bound States
Authors: Bostelmann Henning , Cadamuro Daniela , Mandrysch Jan 
Published: January 2024

AbstractWe investigate lower bounds to the time-smeared energy density, so-called quantum energy inequalities (QEI), in the class of integrable models of quantum field theory. Our main results are a state-independent QEI for models with constant scattering function and a QEI at one-particle level for generic models. In the latter case, we classify the possible form of the stress-energy tensor from first principles and establish a link between the existence of QEIs and the large-rapidity asymptotics of the two-particle form factor of the energy density. Concrete examples include the Bullough–Dodd, the Federbush, and the O(n)-nonlinear sigma models.
Distinct concentration-dependent oxidative stress profiles by cadmium in a rat kidney proximal tubule cell line
Authors: Lee Wing-Kee , Probst Stephanie , Scharner Bettina , Deba Timo , Dahdouh Faouzi , Thévenod Frank 
Published: January 2024

AbstractLevels and chemical species of reactive oxygen/nitrogen species (ROS/RNS) determine oxidative eustress and distress. Abundance of uptake pathways and high oxygen consumption for ATP-dependent transport makes the renal proximal tubule particularly susceptible to cadmium (Cd2+)-induced oxidative stress by targeting ROS/RNS generation or antioxidant defence mechanisms, such as superoxide dismutase (SOD) or H2O2-metabolizing catalase (CAT). Though ROS/RNS are well-evidenced, the role of distinct ROS profiles in Cd2+ concentration-dependent toxicity is not clear. In renal cells, Cd2+ (10–50 µM) oxidized dihydrorhodamine 123, reaching a maximum at 2–3 h. Increases (up to fourfold) in lipid peroxidation by TBARS assay and H2O2 by Amplex Red were evident within 30 min. ROS and loss in cell viability by MTT assay with 50 µM Cd2+ could not be fully reversed by SOD mimetics Tempol and MnTBAP nor by SOD1 overexpression, whereas CAT expression and α-tocopherol were effective. SOD and CAT activities were attenuated below controls only with >6 h 50 µM Cd2+, yet augmented by up to 1.5- and 1.2-fold, respectively, by 10 µM Cd2+. Moreover, 10 µM, but not 25–50 µM Cd2+, caused 1.7-fold increase in superoxide anion (O2•−), detected by dihydroethidium, paralled by loss in cell viability, that was abolished by Tempol, MnTBAP, α-tocopherol and SOD1 or CAT overexpression. H2O2-generating NADPH oxidase 4 (NOX4) was attenuated by ~50% with 10 µM Cd2+ at 3 h compared to upregulation by 50 µM Cd2+ (~1.4-fold, 30 min), which was sustained for 24 h. In summary, O2•− predominates with low–moderate Cd2+, driving an adaptive response, whereas oxidative stress by elevated H2O2 at high Cd2+ triggers cell death signaling pathways.Highlights

Different levels of reactive oxygen species are generated, depending on cadmium concentration.

Superoxide anion predominates and H2O2 is suppressed with low cadmium representing oxidative eustress.

High cadmium fosters H2O2 by inhibiting catalase and increasing NOX4 leading to oxidative distress.

Superoxide dismutase mimetics and overexpression were less effective with high versus low cadmium.

Oxidative stress profile could dictate downstream signalling pathways.
Parental duties and foraging strategies of a tropical pelagic seabird (Phaethon aethereus, Aves: Phaethontidae) during the breeding season
Authors: Piña-Ortiz Alberto , González-Zamora Diego Adolfo , Paz Jesica Andrea , Hernández-Vázquez Salvador , Mellink Eric , Bustamante Paco , Quillfeldt Petra , Castillo-Guerrero José Alfredo 
Published: January 2024

AbstractBreeding seabirds must balance the energetic demands of feeding themselves and their offspring while coping with the constraints imposed by central-place foraging. As such, foraging strategies and parental care patterns are usually linked. Here, the foraging behavior of the Red-billed Tropicbird (Phaethon aethereus; n = 161) of Peña Blanca Islet, Mexico (19° 06ʹ 11ʺ N, 104° 29ʹ 12ʺ W) during the incubation and chick-rearing (≤ 4 weeks of age) stages was characterized with the aid of GPS loggers. Blood samples from adults and chicks were collected to determine δ15N and δ13C, and parental presence at the nest, meal size, and parent-chick feeding events were recorded. During incubation, parents made long trips offshore to areas far from the colony; however, immediately after their chicks hatched, the parents switched to a bimodal foraging strategy by undertaking both short and long foraging trips. The δ15N values indicated that trophic differences were present between parents and their offspring, with chicks being fed prey enriched in 15N. Parental presence at the nest was greater during early chick-rearing, which was associated with a higher provisioning rate. Parents adopted a strategy in which the parent on nest duty only made short foraging trips to provide for its offspring without leaving it unattended for long periods, while its mate undertook long trips to feed itself. After the early chick-rearing period, the parents gradually reduced the time spent at the nest and increased the time spent foraging, compensating with larger meal sizes for their offspring.
Graphical abstract
Musculoskeletal infections through direct inoculation
Authors: Sabir Nuran , Akkaya Zehra 
Published: January 2024

AbstractMusculoskeletal infections consist of different clinical conditions that are commonly encountered in daily clinical settings. As clinical findings and even laboratory tests cannot always be specific, imaging plays a crucial role in the diagnosis and treatment of these cases. Musculoskeletal infections most commonly occur secondary to direct inoculation into the skin involuntarily affected by trauma, microorganism, foreign bodies, or in diabetic ulcers; direct infections can also occur from voluntary causes due to surgery, vaccinations, or other iatrogenic procedures. Hematogenous spread of infection from a remote focus can also be a cause for musculoskeletal infections. Risk factors for soft tissue and bone infections include immunosuppression, old age, corticosteroid use, systemic illnesses, malnutrition, obesity, and burns. Most literature discusses musculoskeletal infections according to the diagnostic tools or forms of infection seen in different soft tissue anatomical planes or bones. This review article aims to evaluate musculoskeletal infections that occur due to direct inoculation to the musculoskeletal tissues, by focusing on the traumatic mechanism with emphasis on the radiological findings.
Pre-assessment of patients with suspected axial spondyloarthritis combining student-led clinics and telemedicine: a qualitative study
Authors: Boy Katharina , von Rohr Sophie , May Susann , Kuhn Sebastian , Schett Georg , Labinsky Hannah , Knitza Johannes , Muehlensiepen Felix 
Published: January 2024

Abstract
Objective
Patients referred to rheumatologists are currently facing months of inefficient waiting time due to the increasing demand and rising workforce shortage. We piloted a pre-assessment of patients with suspected axial spondyloarthritis (axSpA) combining student-led clinics and telemedicine (symptom assessment, symptom monitoring and at-home capillary self-sampling) to improve access to rheumatology care. The aim of this study was to explore (1) current challenges accessing axSpA care and (2) patients’ first-hand experiences.

Methods
Embedded within a clinical trial, this study was based on qualitative interviews with patients with suspected axSpA (n = 20). Data was analysed via qualitative content analysis.

Results
Student-led clinics were perceived as high-quality care, comparable to conventional rheumatologist-led visits. Patients expressed that their interactions with the students instilled a sense of trust. History-taking and examinations were perceived as comprehensive and meticulous. Telehealth tools were seen as empowering, offering immediate and continuous access to symptom assessment at home. Patients reported a lack of specificity of the electronic questionnaires, impeding accurate responses. Patients requested a comments area to supplement questionnaire responses. Some patients reported receiving help to complete the blood collection.

Conclusion
Patients’ access to rheumatology care is becoming increasingly burdensome. Pre-assessment including student-led clinics and telemedicine was highly accepted by patients. Patient interviews provided valuable in-depth feedback to improve the piloted patient pathway.
Effects of transitioning from conventional to organic farming on soil organic carbon and microbial community: a comparison of long-term non-inversion minimum tillage and conventional tillage
Authors: Mihelič Rok , Pintarič Sara , Eler Klemen , Suhadolc Marjetka 
Published: January 2024

AbstractThe combination of conservation tillage (non-inversion and no-till) with organic farming is rare due to weed problems. However, both practices have the potential to improve soil quality and increase soil organic C (SOC). This study investigated the changes in SOC, microbial biomass, and microbial composition during the transition from conventional to organic farming (from 2014 to 2020) in a long-term tillage trial established in 1999. Non-inversion minimum tillage to a depth of 10 cm (MT) resulted in SOC stratification, whilst conventional soil tillage with 25-cm-deep mouldboard ploughing (CT) maintained an even SOC distribution in the plough layer. After 12 years of contrasting tillage in 2011, the uppermost soil layer under MT had a 10% higher SOC content (1.6% w/w) than CT (1.45% w/w). This difference became even more pronounced after introducing organic farming in 2014. By the fall of 2020, the SOC content under MT increased to 1.94%, whilst it decreased slightly to 1.36% under CT, resulting in a 43% difference between the two systems. Conversion to organic farming increased microbial biomass under both tillage systems, whilst SOC remained unchanged in CT. Abundances of total bacterial and Crenarchaeal 16S rRNA and fungal ITS genes indicated shifts in the microbial community in response to tillage and depth. Fungal communities under MT were more responsive to organic farming than bacterial communities. The improved soil quality observed under MT supports its adoption in both organic and conventional systems, but potentially large yield losses due to increased weed cover discourage farmers from combining MT and organic farming.
Similar brain proteomic signatures in Alzheimer’s disease and epilepsy
Authors: Leitner Dominique , Pires Geoffrey , Kavanagh Tomas , Kanshin Evgeny , Askenazi Manor , Ueberheide Beatrix , Devinsky Orrin , Wisniewski Thomas , Drummond Eleanor 
Published: January 2024

AbstractThe prevalence of epilepsy is increased among Alzheimer’s Disease (AD) patients and cognitive impairment is common among people with epilepsy. Epilepsy and AD are linked but the shared pathophysiological changes remain poorly defined. We aim to identify protein differences associated with epilepsy and AD using published proteomics datasets. We observed a highly significant overlap in protein differences in epilepsy and AD: 89% (689/777) of proteins altered in the hippocampus of epilepsy patients were significantly altered in advanced AD. Of the proteins altered in both epilepsy and AD, 340 were altered in the same direction, while 216 proteins were altered in the opposite direction. Synapse and mitochondrial proteins were markedly decreased in epilepsy and AD, suggesting common disease mechanisms. In contrast, ribosome proteins were increased in epilepsy but decreased in AD. Notably, many of the proteins altered in epilepsy interact with tau or are regulated by tau expression. This suggests that tau likely mediates common protein changes in epilepsy and AD. Immunohistochemistry for Aβ and multiple phosphorylated tau species (pTau396/404, pTau217, pTau231) showed a trend for increased intraneuronal pTau217 and pTau231 but no phosphorylated tau aggregates or amyloid plaques in epilepsy hippocampal sections. Our results provide insights into common mechanisms in epilepsy and AD and highlights the potential role of tau in mediating common pathological protein changes in epilepsy and AD.
Optimierung der Klauswehrordnung des Mondsees, Teil II – Hydrologie
Authors: Klingler C. , Preiml M. , Habersack H. , Holzmann H. 
Published: January 2024

ZusammenfassungSeen mit großer Oberfläche weisen grundsätzlich ein hohes Retentionspotenzial auf und beeinflussen das Abflussgeschehen der Unterlieger-Bereiche. Der Wasserstand bzw. der Abfluss des Mondsees wird durch ein steuerbares Klauswehr im Nieder- und Mittelwasserbereich geregelt, dessen Betriebsweise durch eine Wehrordnung aus dem Jahr 1982 festgelegt ist. Verschiedene Nutzeransprüche verfolgen hinsichtlich der Wasserstände und Abflüsse unterschiedliche Interessen. In diesem Beitrag werden die Grundlagen für eine adaptierte Klauswehrordnung vorgestellt, welche nicht nur die Verbesserung des Hochwasserschutzes, sondern auch andere Nutzeransprüche berücksichtigen. Gegenüber ersten Überlegungen aus einem Vorprojekt, in dem die Möglichkeit der prognosebasierten Vorabsenkung untersucht wurde, fokussiert dieser Beitrag auf eine saisonal optimierte Betriebsweise des Klauswehrs. Damit kann sowohl die Hochwassergefährdung durch hohe Seewasserstände verbessert, aber auch andere Nutzungen wie Energiewirtschaft, Tourismus und Ökologie positiv beeinflusst werden.
The intersection of oncology and oral health: exploring nurses’ insights and practices — a systematic review
Authors: Sajwani Arsheen Imran , AlShdaifat Mohammad , Hashi Fatima , Abdelghany Eman , Alananzeh Ibrahim 
Published: January 2024

Abstract
Purpose
Oral health care for cancer patients is essential but often overlooked. Nurses play a critical role in assessing and managing oral health in this population. This systematic review aims to examine nurses’ knowledge, attitudes, and practices regarding oral healthcare in cancer patients.

Methods
A systematic review was conducted following the Joanna Briggs Institute methodology. Qualitative and quantitative studies focused on nurses’ knowledge, attitudes, and practices in oral healthcare for cancer patients. Seven databases were searched for studies published between January 2000 and January 2023. The primary outcomes of interest were patient satisfaction, quality of life, and nurses’ knowledge, attitudes, and practices related to oral healthcare.

Results
The review identified gaps in nurses’ knowledge and training in oral healthcare for cancer patients. Insufficient understanding of oral diagnoses, treatment protocols, and pediatric oral care was noted. Lack of knowledge and skills posed barriers to implementation. Some healthcare providers demonstrated low awareness of oral health recommendations, including the use of fluoridated toothpaste and the need for dental referrals. Referrals to dental services and regular oral assessments were infrequent. Attitudes towards oral healthcare varied, with providers feeling more comfortable in certain areas than others.

Conclusion
Enhancing nurses’ knowledge, attitudes, and practices in oral healthcare for cancer patients is crucial. Targeted educational initiatives and interventions are needed to address these gaps. By improving nurses’ understanding of oral complications and management approaches, patient outcomes and quality of life can be improved.
Registration: PROSPERO International prospective register of systematic reviews, ID: CRD42022368053.
An intelligent Island detection scheme to enhance grid resilience
Authors: Shukla Apoorva , Dutta Soham , Sadhu Pradip Kumar , Dey Bishwajit 
Published: January 2024

AbstractThe importance of strengthening grid resilience has grown with the increase in environmental destruction and modern power grid complexity, as a consequence of power outages inflicted by human intrusion and extreme weather events. Micro-grids (MGs) have proven to be a viable alternative in such circumstances. However, these occurrences are highly unpredictable, resulting in unintended islands of MGs with negative consequences. As a response, alerting its distributed generations about unintended island is indeed a crucial issue for enhancing grid resilience with MG. Therefore, it is essential to develop a technique for the efficient and accurate detection of unintended islands. There has been an increase in the use of micro-phasor measurement units (µ-PMUs) in MG. In the perspective of this, using an efficient µ-PMU, the research provides a method for finding unintended islands in a MG. The µ-PMU analyses the solar generator bus voltage and analyzes it with symmetrical components for island identification. This study introduces a µ-PMU based Fortescue-transform and random forest algorithm method for rapid detection of unintended islanding in distribution generation system. The approach monitors voltage phasor of zero and negative sequence, calculating angular sum over time to distinguish islanding event from other disturbance. Using Matlab/Simulink, the proposed method is evaluated on the IEEE-34 node distribution network. Multiple simulations provide validation for the method’s resilient performance. The methodology proposed has a detection time of 20 ms.
On the settling of a spherical particle in slightly perturbed ambient fluid
Authors: Catalán J. M. , Moriche M. , Flores O. , García-Villalba M. 
Published: January 2024

AbstractDirect numerical simulations of the settling of a spherical particle under the action of gravity in a slightly perturbed ambient fluid have been performed. The ambient perturbations are generated using a synthetic turbulence inflow generator method, and their length scale and intensity are varied to study their influence on the particle motion. The Galileo number is 151 and the solid-to-fluid density ratio is 1.5, so that in the absence of perturbations, the particle settles following a steady vertical trajectory. It has been found that the ambient perturbations trigger the formation of double-threaded vortical structures in the wake of the particle. These structures resemble those that appear in the oblique oscillating regime that is found in the absence of perturbations at higher Galileo numbers. Due to the flow perturbations the particle is pushed randomly in all directions, and this results in a combination of slow lateral drifts along fixed directions and relatively fast excursions in random directions. The particle response has been characterized using probability density functions of the velocity in the cross-plane and persistence probability. The slow drifts are strongly influenced by the size of the perturbations and by the rotational motion of the particle, while the intensity of the perturbations seems to play a minor role.
Comparison of laser ablation, simple excision, and flap reconstruction in the treatment of pilonidal sinus disease
Authors: Tyrväinen Emmi , Nuutinen Henrik , Savikkomaa Elina , Myllykangas Heidi-Mari 
Published: January 2024

Abstract
Pilonidal sinus disease (PSD) is a common condition that typically affects young adults. PSD may cause significant morbidity due to its chronic nature and tendency to recur. Laser ablation has recently shown promising results in the treatment of PSD, but comparative studies are sparse. We aimed to compare laser ablation with two conventional treatment options: simple excision with direct closure and excision with flap reconstruction. This retrospective study material included patients who underwent PSD surgery in the plastic surgery department of a single academic teaching hospital. Patients were divided into three groups based on the operation technique: laser group, direct closure group, and flap group. Preoperative and postoperative data were compared between the groups including patient characteristics, residual disease, recurrent disease, complications, and re-operations. Among the 278 patients, 66 underwent laser treatment, 134 excision with direct closure, and 78 excision with flap closure. The follow-up time ranged from 15.4 ± 7.6 months in the laser group to 87.6 ± 29.3 months in the flap group. Eventless healing occurred in 67.7% of the patients in the laser group, 66.4% of the patients in the direct closure group, and 56.4% of the patients in the flap group. There was significantly more residual disease in the laser group whereas significantly more complications were found in the direct closure and flap groups. The advantages of laser treatment include fast postoperative recovery and reduced risk of complications.