bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum transferrin receptor in the evaluation of the iron status in elderly hospitalized patients with anemia
Authors: Joosten Etienne , van Loon Ronny , Billen Jaak , Blanckaert Norbert , Fabri Ria , Pelemans Walter 
Published: August 2002

AbstractThe aim of the present study is to evaluate in an elderly hospitalized population the diagnostic value of the serum transferrin receptor (sTfR) in distinguishing IDA (iron deficiency anemia) from ACD (anemia of chronic disease) as compared to conventional laboratory tests of iron metabolism, especially serum ferritin. In a prospective study, 34 patients with IDA and 38 patients with ACD (a chronic disorder in 23 and an acute infection in 15) were evaluated using iron status tests including serum transferrin receptor assay. The iron stores were assessed by bone marrow examination. sTfR levels were elevated (>28.1 nmol/L) in 68% of the IDA patients but also in 43% of the patients with ACD‐chronic inflammation and 33% with ACD‐acute infection. Serum ferritin was the best test to differentiate IDA from ACD patients. We conclude that serum ferritin is a more sensitive and specific parameter than the sTfR assay to predict the bone marrow iron status in an elderly anemic population. Am. J. Hematol. 69:1‐6, 2002. © 2002 Wiley‐Liss, Inc.
Effectiveness of platelet transfusions after plasma exchange in adult thrombotic thrombocytopenic purpura: A report of two cases
Authors: Coppo P. , Lassoued K. , Mariette X. , Gossot D. , Oksenhendler E. , Adrie C. , Azoulay E. , Schlemmer B. , Clauvel J.P. , Bussel A. 
Published: August 2002

AbstractPlasma infusion (PI) and plasma exchange (PE) are the most efficient treatment of thrombotic thrombocytopenic purpura (TTP), allowing achievement of complete remission in 60 to 90% of cases. Life‐threatening bleeding, related to severe thrombocytopenia, is one of the main complications of the disease. Thrombocytopenia may also preclude invasive procedures such as splenectomy, which may be required during the management of TTP. Platelet concentrates transfusions are usually thought to worsen the disease, especially if not associated with the appropriate treatment of this latter, and thus should be avoided. We report hereon 2 patients with TTP who experienced a surgical procedure i.e., a cholecystectomy for a cholecystitis, and a splenectomy for a refractory TTP. In both patients, the surgical procedure was preceded by a 60 mL/kg plasma exchange with solvent/detergent treated plasma as replacement fluid, followed by platelet transfusion, with a corrected count increment of 57.1% (Patient 1) and 69.3% (Patient 2). Using this sequential treatment, the patients did not experience any deterioration of their status. Both patients had a favorable outcome after surgery. However, until such a procedure will be validated on a larger series of patients, it should be restricted to patients presenting with a refractory life‐threatening thrombocytopenia and/or requiring surgery or any kind of invasive procedure. Am. J. Hematol. 68:198–201, 2001. Published 2001 Wiley‐Liss, Inc.
Genetic variability in the Guahibo population from venezuela
Authors: Moral Pedro , Marini Elisabetta , Estebanup Esther , Mameli Giuseppa Elisa , Succa Valeria , Vona Giuseppe 
Published: August 2002

AbstractFour communities from Guahibo of Venezuela were analyzed for the genetic variants of nine erythrocyte enzymes and five serum proteins. Of the 14 loci determined, four were monomorphic. Significant frequency differentiation among communities, was present for ESD and TF markers. In general, Guahibo allele frequencies are in the variation ranges described for South American groups. The analysis indicates a relatively higher affinity of Guahibos with other Venezuelan groups within an irregular pattern of genetic distances that are likely related to the complex demographic history of the South American groups. Genetic diversity estimates reveal a moderate degree of genetic structure between the four Guahibo communities. This intra‐tribal variability in Guahibo appears to be lower than in Venezuelan Piaroa but higher than in other Amerindians and could be attributed to a combined effect of low population size and relative isolation of communities. At a continental level, the distribution of genetic diversity is consistent with preferential population movements along the eastern and western coastal areas. Am. J. Hum. Biol. 14:21–28, 2002. © 2002 Wiley‐Liss, Inc.
Association studies of the CT repeat polymorphism in the 5′ upstream region of the cholecystokinin B receptor gene with panic disorder and schizophrenia in Japanese subjects
Authors: Hattori Eiji , Yamada Kazuo , Toyota Tomoko , Yoshitsugu Kiyoshi , Toru Michio , Shibuya Haruo , Yoshikawa Takeo 
Published: August 2002

AbstractThe tetrapeptide of cholecystokinin (CCK), CCK‐4, is known to induce panic attacks in human subjects, while CCK‐8 is reported to have a therapeutic effect on schizophrenia symptoms. Recently, we have identified a novel microsatellite polymorphism in the 5′ upstream region of the CCK gene and shown a significant association between this polymorphism and panic disorder. In this study, we have investigated the CCK‐B receptor (CCKBR) gene, which is the main constituent of the CCK receptor in the CNS. Recently, a dinucleotide repeat, (CT)n, in the 5′ regulatory region of the CCKBR gene was reported to be associated with panic disorder in Canadian samples. To evaluate an association of the CT repeat with panic disorder and schizophrenia, we genotyped 71 subjects with panic disorder, 154 schizophrenics and 199 controls. However, no evidence of allelic association was found between the polymorphic repeat of the CCKBR gene and either panic disorder or schizophrenia (P = 0.186 and 0.987, respectively). Together with the negative reports on association analyses using other polymorphisms of the CCKBR gene and Japanese samples, the present results exclude a major genetic contribution of the CCKBR gene to susceptibilities to panic disorder and schizophrenia in Japanese cohorts. © 2001 Wiley‐Liss, Inc.
Mixed clefting type in Rapp‐Hodgkin syndrome
Authors: Neilson Derek E. , Brunger Jeanne W. , Heeger Shauna , Bamshad Michael , Robin Nathaniel H. 
Published: August 2002

AbstractMixed clefting type (MCT) is the rare occurrence of cleft lip, with or without cleft palate, and cleft palate alone in the same pedigree. Here we present a family with Rapp‐Hodgkin syndrome (RHS) that manifests MCT, and use this rare finding to suggest that RHS may be related not only to phenotypically similar syndromes, but seemingly dissimilar ones as well. RHS has obvious phenotypic overlap with other ectodermal dysplasia‐clefting syndromes (EDCS), such as ectrodactyly‐ectodermal dysplasia‐clefting syndrome (EEC) and ankyloblepharon‐ectodermal dysplasia‐clefting syndrome (AEC), all of which show MCT. MCT is also found in the allelic disorders van der Woude syndrome (VDW) and popliteal‐pterygium syndrome (PPS). Therefore, while VDW and PPS have little clinical overlap with the EDCS, the common finding of MCT may indicate closer relationships at the developmental or genetic level. © 2002 Wiley‐Liss, Inc.
Niemann‐Pick disease type B: An unusual clinical presentation with multiple vertebral fractures*
Authors: Volders P. , Van Hove J. , Lories R.J.U. , Vandekerckhove Ph. , Matthijs G. , De Vos R. , Vanier M.T. , Vincent M.F. , Westhovens R. , Luyten F.P. 
Published: August 2002

AbstractWe report here a unique case of a 55‐year‐old woman presenting with a clinical picture of Parkinson disease, severe back pain, splenomegaly, and pronounced dyspnea. Radiographic examination of the spine showed multiple vertebral fractures. Niemann‐Pick disease type B was diagnosed by findings of lipid‐loaded histiocytes and a strongly reduced sphingomyelinase enzyme activity. She was homozygous for the deletion of codon 608 (delR608), which encodes an arginine residue in the Acid Sphingomyelinase gene. To investigate the cause of the unusual vertebral fractures, we screened for polymorphisms previously described as possibly associated with increased risk for osteoporosis and fractures. Our patient was heterozygous for the polymorphisms of the vitamin D receptor gene, the estrogen receptor gene, and the collagen 1A1gene. Increased physical activity after Parkinson treatment, a genetic predisposition, together with worsening disease due to interfering medications could explain the dramatic presentation of this patient. She was treated with cholesterol lowering drugs such as statins to decrease sphingomyelin synthesis, avoidance of drugs that inhibit sphingomyelinase, and bisphosphonates. No new fractures have occurred, but the interstitial lung disease has progressed. © 2002 Wiley‐Liss, Inc.
Duplication of chromosome region (16)(p11.2 → p12.1) in a mother and daughter with mild mental retardation
Authors: Engelen J.J.M. , de Die‐Smulders C.E.M. , Dirckx R. , Verhoeven W.M.A. , Tuinier S. , Curfs L.M.G. , Hamers A.J.H. 
Published: August 2002

AbstractWe report a 40‐year‐old female with mild mental retardation and behavior problems and her 6‐year‐old daughter. Chromosome analysis showed that both patients had a proximal duplication in the short arm of chromosome 16. The aberration was characterized further with band‐specific probes, resulting in a 46,XX,dir dup(16)(pter → p11.2::p12.1 → qter) karyotype. The clinical and cytogenetical findings are compared to other patients with partial trisomy 16p reported in the literature. © 2002 Wiley‐Liss, Inc.
Phenotypic spectrum of interstitial 7p duplication in mosaic and non‐mosaic forms
Authors: Cox Helen , Stewart Helen , Hall Lucy , Donnai Dian 
Published: August 2002

AbstractThe phenotypes of a mother and child with a duplication of 7p15–7p22 are described. The mother is mosaic for the cytogenetic abnormality, whereas all cells are affected in her son. Fewer than 5 patients with interstitial 7p duplications are described in the world literature whereas over 30 phenotypic descriptions of individuals with terminal 7p duplication can be found. Authors have suggested that the associated phenotype amounts to a recognizable syndrome. The current cases give further insights into the phenotype that results from pure 7p duplication, both in its mosaic and in its full form. Comparisons are made with previous cases, in the light of the shorter segment involved in the current patients, whose duplication does not extend to pter. This case description will be useful in counseling patients with duplications of 7p and lends support to the existence of characteristic craniofacial features and congenital malformations in this chromosome rearrangement. In addition, as earlier case reports all describe the phenotype associated with non‐mosaic partial 7p trisomy, the current observations amount to clear evidence that mosaicism attenuates the phenotype of this rearrangement. © 2002 Wiley‐Liss, Inc.
Systematic search for mutations in the human tissue inhibitor of metalloproteinases‐3 (TIMP‐3) gene on chromosome 22 and association study with schizophrenia
Authors: Hung Chao‐Chun , Chen Yun‐Hsiang , Tsai Ming‐Ta , Chen Chia‐Hsiang 
Published: August 2002

AbstractSeveral linkage studies have suggested that chromosome 22q12–q13 is a putative region for schizophrenic genes. In this study, the human tissue inhibitor of metalloproteinase‐3 (TIMP‐3) gene was investigated as positional candidate gene for schizophrenia because of its regulatory function on extracellular matrix proteins, cell adhesion molecules, and neural cell adhesion molecules in the brain. We systematically searched for the nucleotide variants by sequencing all the exons and their flanking intronic sequences in a sample of Chinese schizophrenic patients from Taiwan. Two silent mutations in the exon 3 were identified: c.249T→C at codon 83 (His) and c.261C→T at codon 87 (Ser). However, no mutations causing amino acid alteration or aberrant splicing of transcripts were observed. Hence, it is unlikely that the TIMP‐3 gene itself may play an important role in the genetic susceptibility to schizophrenia. Further case control association study revealed a significant difference of genotype distribution of the c.249T→C between schizophrenic patients and control. This finding supports that 22q12 is a schizophrenia susceptible region, and it is likely that there might be other genetic mutations in the neighborhood of the TIMP‐3 gene locus that may contribute to the susceptibility of schizophrenia. © 2001 Wiley‐Liss, Inc.
Analysis of epistasis in linked regions in the Irish study of high‐density schizophrenia families
Authors: Sullivan Patrick F. , O'Neill F. Anthony , Walsh Dermot , Ma Yunlong , Kendler Kenneth S. , Straub Richard E. 
Published: August 2002

AbstractEpistasis may be important in the etiology of schizophrenia. Analysis of epistasis has been important in the positional cloning of a gene involved in the etiology of type II diabetes mellitus. We investigated the importance of epistasis among six linked regions in 268 multiplex pedigrees in the Irish Study of High‐Density Schizophrenia Families (ISHDSF) by computing pairwise correlations between nonparametric linkage scores for narrow, intermediate, and broad diagnostic definitions. The linked regions were on chromosomes 2, 4, 5, 6, 8, and 10. No correlation reached our a priori level of statistical significance. Using this statistical approach, we did not find evidence of important epistatic effects among these six regions in the ISHDSF. © 2001 Wiley‐Liss, Inc.
Tool‐using chimpanzees
Authors: Mason William A. 
Published: August 2002

AbstractReview of Folk Physics for Apes. The Chimpanzee’s Theory of How the World Works by Daniel J. Povinelli, in collaboration with James E. Reaux, Laura A. Theall, and Steve Giambrone. Oxford, Oxford University Press, 2000, xiii + 391 pp, 98 fig., 35 tab., $85.00.
Infant feeding and weaning practices in Roman Egypt
Authors: Dupras Tosha L. , Schwarcz Henry P. , Fairgrieve Scott I. 
Published: August 2002

AbstractCurrent knowledge of infant feeding and weaning practices during the Roman period in Egypt is limited to scanty documentary and iconographic evidence. Stable nitrogen and carbon isotope analysis provides another avenue to explore this question. A sample of 49 infant and juvenile human skeletal remains from the Kellis 2 cemetery in the Dakhleh Oasis, Egypt, was used to determine patterns of infant feeding and weaning. δ15N values indicate that supplementary foods were introduced at around 6 months of age, and that weaning was complete by 3 years of age. By 6 months of age, δ13C values become increasingly enriched over adult values, and reach peak enrichment at approximately 1.5 years of age. Beyond this age, δ13C gradually declines to approach adult values. This enrichment in infant δ13C values is indicative of consumption of 13C‐enriched supplementary foods. Based on isotopic study of faunal and botanical remains from the ancient village of Kellis, we conclude that at approximately 6 months of age, infants were fed milk of goat and/or cow. Am J Phys Anthropol 115:204–212, 2001. © 2001 Wiley‐Liss, Inc.
Mouse glioma gene expression profiling identifies novel human glioma‐associated genes
Authors: Gutmann David H. , Huang Zhi‐Yong , Hedrick Nicolé M. , Ding Hao , Guha Abhijit , Watson Mark A. 
Published: August 2002

AbstractBased on previous studies demonstrating increased RAS activity in human astrocytomas, we developed a transgenic mouse model (B8) that targets an activated RAS molecule to astrocytes. Within 3 to 4 months after birth, these mice develop high‐grade astrocytomas that are histologically identical to human astrocytomas. To characterize genetic events associated with B8 mouse astrocytoma formation, we employed comparative gene expression profiling of wild‐type cultured mouse astrocytes, non‐neoplastic B8 astrocytes, B8 astrocytoma cultures, and two other astrocytoma cultures from independently derived RAS transgenic mouse lines. We identified several classes of gene expression changes, including those associated with the non‐neoplastic state in the B8 transgenic mouse, those associated with astrocytoma formation, and those specifically associated with only one of the three independently derived transgenic mouse astrocytomas. Differential expression of several unique genes was confirmed at the protein level in both the RAS transgenic mouse astrocytomas and two human glioblastoma multiforme cell lines. Furthermore, reexpression of one of these downregulated astrocytoma‐associated proteins, GAP43, resulted in C6 glioma cell growth suppression. The use of this transgenic mouse model to identify novel genetic changes that might underlie the pathogenesis of human high‐grade astrocytomas provides a unique opportunity to discover future targets for brain tumor therapy.
Synthesis and properties of σ–π conjugated alternating polymers consisting of carbazole and organosilicon units
Authors: Sakamaki Koichi , Ohshita Joji , Kunai Atsutaka , Nakao Hidenobu , Adachi Akira , Okita Koichi 
Published: August 2002

AbstractWe have synthesized novel σ–π conjugated polymers with an alternating organosilanylene and π‐electron system, intending to utilize them for hole‐transporting materials of electroluminescent (EL) devices. 3,6‐Di(lithioethynyl)carbazoles were co‐polymerized with organodichlorosilanes to give the corresponding polymers with molecular weights of MW = 2000–5000. Another type of polymer with a thienylene unit was also synthesized by the nickel‐catalyzed reaction of the di‐Grignard reagent of 1,2‐bis[2‐(5‐bromothienyl)]tetraethyldisilane with 3,6‐dibromocarbazole, the molecular weight being Mn = 3100. The EL devices with a double‐layer system composed of tris(8‐quinolinolato)aluminum(III) and the present polymers as the emitting‐electron‐transporting and hole‐transporting layers, respectively, emit green EL with a maximum intensity of the order of 103 cd m−2. Of these, the device with the thienylene–carbazole polymers exhibited the highest luminance of 1480 cd m−2. Copyright © 2001 John Wiley & Sons, Ltd.
Aluminiumorganic compounds of some metaldioximates
Authors: Voiculescu Nicolae 
Published: August 2002

AbstractCoordinated dioximes with nickel(II), palla‐dium(II) and copper(II) ions proved capable of giving substitution reactions with a series of aluminiumorganic compounds. Studies made on the reaction products have revealed that in the case of both dimethylglyoxime and diphenylglyoxime. The reaction products result from substitution of the OH…O hydrogen bridges existing in such compounds by OAlO bridges. All compounds reported in this paper were separated from the reaction mixture as coloured powders and were characterized by chemical analyses, IR spectroscopy, X‐ray diffraction spectra, proton NMR spectra and magnetic properties. Copyright © 2001 John Wiley & Sons, Ltd.
Dimethylarsinic acid targets tubulin in mitotic cells to induce abnormal spindles
Authors: Kawata Hiroko , Kuroda Koichi , Endo Yoko , Endo Ginji 
Published: August 2002

AbstractDimethylarsinic acid (DMA) is the most effective inducer of cell‐cycle disruption among the arsenic compounds and their metabolites. The present study was conducted to gain further insight into cell‐cycle disruption induced by DMA. The inhibition of cell proliferation and the mitotic arrest induced by DMA were significant and dose‐dependent in Chinese hamster V79 cells and the two seemed to be closely related. At less than 140 µM the DMA did not inhibit the proliferation of cells, but it significantly induced mitotic arrest. An indirect immunofluorescence assay using anti‐α‐tubulin antibodies revealed that DMA induced the formation of abnormal spindles in the metaphase cells even at 350 µM with 5 h of treatment. At 1.4 mM DMA no metaphase cells could form a definite spindle structure. The spindle figures were similar to those induced by colchicine (125 nM) or vinblastine (110 nM), major antimitotic agents. In DMA‐treated interphase cells, the microtubule networks were indistinguishable from those of normal cells. With the tubulin‐assembly assay estimated by turbidity, DMA at less than 200 µM suppressed tubulin assembly in a dose‐dependent manner, whereas at more than 700 µM it enhanced tubulin polymerization remarkably with or without addition of excess guanosine‐5′‐triphosphate. According to the above findings, we discussed the possibility that DMA, a primary metabolite of inorganic arsenic in mammals, is related to arsenic carcinogenicity. Copyright © 2001 John Wiley & Sons, Ltd.
Organometallic complexes with biological molecules: XVI. Endocrine disruption effects of tributyltin(IV)chloride on metamorphosis of the ascidian larva
Authors: Patricolo E. , Mansueto C. , D'Agati P. , Pellerito L. 
Published: August 2002

AbstractThe effects of tributyltin(IV)chloride (TBT) on the metamorphosis of ascidian larvae of Ciona intestinalis, 2 h after hatching, were investigated. Ascidians are protochordates that lack thyroid follicles and possess thyroid hormones (THs) and their precursors, 3‐monoiodo‐tyrosine (MIT) and 3,5‐diiodo‐tyrosine (DIT), in their endostyle. According to recent findings, these hormones are also present at larval stages, localize in mesenchymal cells and their function seems to be mainly related to larval transformations. Here, we investigate the effects of TBT on thyroxine (T4) content and localization by exposing larvae of C. intestinalis for different times to TBT concentrations known to block metamorphosis. The result is a blocking of the retraction of the tail, and larval immobility. As detected by immmunohistochemistry, in normal larvae, T4 is found in all mesenchymal cells spread out in the body cavity, under the adhesive papillae and around the intestine. By contrast, in larvae exposed to 10−5 M and 10−7 M TBT, T4 is detected only in 5% and 25% of total mesenchymal cells respectively. Radioimmunoassay shows a 70% decrease of T4 content in TBT‐exposed larvae, with respect to unexposed larvae. In addition, neosynthesis of THs is inhibited. Even if parallelism does not exist between the endocrine systems of invertebrates and vertebrates, however, a close similarity exists for functions such as reproduction and metabolism. Our results indicate that TBT could behave as an endocrine disrupter (ED) in ascidians and could impair T4 metabolism. These findings suggest that the ED activity of TBT could be conserved from invertebrates to vertebrates. Copyright © 2001 John Wiley & Sons, Ltd.
Aluminiumorganic compounds of coordinated salicylaldoxime and their adducts with nitrogen Lewis bases
Authors: Voiculescu Nicolae 
Published: August 2002

AbstractAluminiumorganic compounds of a coordinated salicylaldoxime resulted from reaction of M(II)(SALOxH)2 (where M(II) = Ni(II), Pd(II) and Cu(II); SALOx represents the divalent radical of the salicylaldoxime) with AlR3(R = CH3,C2H5,i‐C4H9,C6H5 and Cl). Copper(II) bis‐salicylaldoximate reacting with Al(i‐C4H9)3 does not form a compound similar to those obtained with nickel and palladium. Aluminiumorganic compounds of the coordinated salicylaldoxime result from the substitution of OHO hydrogen bonds, existing in chelates, by OAlO bridges. All compounds reported in this paper were separated from the reaction mixture as coloured powders and were characterized by chemical analyses, IR spectroscopy, X‐ray diffraction spectra, proton NMR spectra and magnetic properties. The new aluminiumorganic compounds form adducts with amine. Among the amine adducts, only the adducts with pyridine were isolated to confirm their formula and the mode of binding. Copyright © 2001 John Wiley & Sons, Ltd.