bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caregivers' job stress related to oral health care of the elderly in long-term care facilities during a prolonged COVID-19 pandemic
Authors: Park Ji-A , Han Sol , Paik Hye-Ran , Lee Jae-Young , Jin Bo-Hyoung 
Published: October 2023

Abstract

Background The COVID-19 pandemic has been a great ordeal for elderly individuals and their caregivers. Oral health care for the elderly during the pandemic would be burdensome for those in long-term care facilities. Therefore, we confirmed caregivers’ behavioral practices and stress related to oral health care and infection control for the elderly in long-term care facilities after the COVID-19 outbreak, their relationship with job evaluations, and changes according to the prolonged pandemic. Methods Two surveys were administered to 215 and 201 caregivers working in long-term care facilities in 2020 and 2022, respectively, during the COVID-19 pandemic. The surveys assessed caregivers’ sociodemographic characteristics and job status, infection control behaviors, psychological stress in providing oral health care to the elderly during the COVID-19 pandemic, overall job stress and job satisfaction in elderly care, and turnover intentions. We analyzed the pattern in each year, the changing trend from 2020 to 2022, and the correlations among them for the behavioral and psychological impact on caregivers by the COVID-19 outbreak using an online surveying system. Results After the COVID-19 outbreak, infection control behaviors (personal protection and cross-infection prevention measures) in providing oral health care to the elderly increased rapidly. The most significant stressor for caregivers in this work was the limitation of their daily lives, followed by the challenge of managing the oral health problems of elderly individuals. A low practice rate of infection control behaviors and high levels of stress related to oral health care and elderly care jobs were correlated with low job satisfaction levels and high turnover intention. As the pandemic extended over three years from 2020 to 2022, infection control behavior practice further improved, and all stressors (e.g., fear, burden, and difficulty) in oral health care for the elderly declined. Although overall job stress in older people’s healthcare was similar, job satisfaction decreased, and serious concerns about job turnover increased significantly. Conclusions We identified behavioral and psychological stress and changes in the oral health care of caregivers for older people during the COVID-19 pandemic. Based on these results, we should have a system to support caregivers who care for the elderly to maintain their oral health and quality of life in response to future public health crises.
Novel biomarkers involved in neuroblastoma revealed by integrative analysis of GEO data
Authors: Xiong Zijun , Xu Mingjun , Yuan Ping , Yu Kefei , Xing Huanhuan , Qiu Liangyu , Yang Ruofan , Zhang Pu , Li Qiang , Zhang Jun , Wang Zihan , Zhao Liang , Gu Jiaowei , Liu Wenting 
Published: October 2023

Abstract

In this study, comprehensive bioinformatics analysis was used to identify differentially expressed genes (DEGs) between neuroblastoma cancer tissues and normal tissues, and to screen the hub genes related to neuroblastoma. GSE54720 and GSE78061 datasets were downloaded from the Gene Expression Omnibus (GEO) database to screen DEGs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on common DEGs. The protein-protein interaction (PPI) network was constructed using STRING database and Cytoscape software, and the top15 hub genes were screened out. Finally, KIF5C, TAGLN3, and SNAP91 were identified by alignment in OMIM, DisGeNET, GeneCards databases, and PubMed. These three genes are neuroblastoma related genes that have never been reported in the literature and experimentally validated. We identified a total of 37 common DEGs from the two microarray databases. KEGG pathway analysis showed that these DEGs were mainly involved in dopaminergic synapses, motor proteins and phenylalanine metabolism related pathways. GO enrichment analysis showed that KIF5C, TAGLN3 and SNAP91 were mainly concentrated in axon guidance, axon genesis, axon development, distal axon, neuronal cell body, and synaptic vesicle transport, suggesting that they may be involved in biological functions such as protein binding, plasma membrane, membrane composition and nucleus. Through OMIM, DisGeNET, GeneCards databases, and PubMed, we found that neuroblastoma related genes KIF5C, TAGLN3, and SNAP91 are associated with the proliferation and migration of other tumors. The identification of hub genes and molecules by bioinformatics methods in this study helps to reveal the potential pathogenic mechanism of neuroblastoma. These genes may be used as diagnostic indicators and therapeutic biomarkers for neuroblastoma, thereby improving the understanding of the molecular mechanism of neuroblastoma.
Association between physiological factors and grip and leg extension strength for predicting health outcomes: Tohoku Medical Megabank Community-based cohort study
Authors: Noji Yoshiaki , Hatanaka Rieko , Nakaya Naoki , Kogure Mana , Nakaya Kumi , Chiba Ippei , Kanno Ikumi , Nakamura Tomohiro , Tsuchiya Naho , Momma Haruki , Hamanaka Yohei , Orui Masatsugu , Kobayashi Tomoko , Uruno Akira , Kodama Eiichi N , Nagatomi Ryoichi , Fuse Nobuo , Kuriyama Shinichi , Hozawa Atsushi 
Published: October 2023

Abstract
Background
Upper and lower extremity muscle strengths predict health outcomes. However, the difference between each muscle's strength in physiological factors affecting the causal association is unclear. We aimed to evaluate the association between physiological data and muscle strength, measured using the grip and leg extension strengths, among Japanese adults.
Methods
We conducted a cross-sectional study of 2,861 men and 6,717 women aged ≥ 20 years, who lived in Miyagi Prefecture, Japan. The grip strength was measured using a dynamometer. The leg extension strength was measured using a hydraulic isokinetic leg press machine. Anthropometry and physiological data were assessed, including blood pressure, calcaneal ultrasound bone status, pulmonary function, carotid echography, and blood information. We used a general linear model adjusted for age, body composition, and smoking status to evaluate the association between muscle strength and physiological factors.
Results
Both grip and leg extension strengths were positively associated with the bone area ratio, vital capacity, forced vital capacity, forced expiratory volume in one second and eGFR, and negatively associated with the waist circumference and percentage body fat mass in both sexes. The diastolic blood pressure was positively associated with grip strength in both sexes and leg extension strength in men but not with leg extension strength in women. HDL cholesterol and red blood cell counts were positively associated with grip strength and leg extension strength in women, but not in men. In both sexes, the pulse rate, total cholesterol, and uric acid were consistently associated with only leg extension strength but not grip strength. In women, glycated hemoglobin demonstrated negative and positive associations with the grip and leg extension strengths, respectively.
Conclusions
The grip strength and leg extension strength demonstrated similar associations with anthropometry, pulmonary function, and eGFR, but the associations with the other factors were not always consistent. The impact of muscle strength on physiological data may partially follow different paths for the grip and leg extension strengths.
Trial Registration
Not applicable
Development of a rapid and comprehensive genomic profiling test supporting diagnosis and research for brain tumors
Authors: Nakashima Takuma , Yamamoto Ryo , Ohno Makoto , Sugino Hirokazu , Takahashi Masamichi , Funakoshi Yusuke , Nambu Shohei , Uneda Atsuhito , Yanagisawa Shunsuke , Uzuka Takeo , Arakawa Yoshiki , Hanaya Ryosuke , Ishida Joji , Yoshimoto Koji , Saito Ryuta , Narita Yoshitaka , Suzuki Hiromichi 
Published: October 2023

Abstract
A prompt and reliable molecular diagnosis for brain tumors has become crucial in precision medicine. While Comprehensive Genomic Profiling (CGP) has become feasible, there remains room for enhancement in brain tumor diagnosis due to the partial lack of essential genes and limitations in broad copy number analysis. Additionally, the long turnaround time of commercially available CGPs poses an additional obstacle to the timely implementation of results in clinics. To address these challenges, we developed a CGP encompassing 113 genes, genome-wide copy number changes, and MGMTpromoter methylation. Our CGP incorporates not only diagnostic genes but also supplementary genes valuable for research. Our CGP enables us to simultaneous identification of mutations, gene fusions, focal and broad copy number alterations, and MGMT promoter methylation status, with results delivered within a minimum of four days. Validation of our CGP, through comparisons with whole-genome sequencing, RNA sequencing, and pyrosequencing, has certified its accuracy and reliability. We applied our CGP for 23 consecutive cases of intracranial mass lesions, which demonstrated its efficacy in aiding diagnosis and prognostication. Our CGP offers a comprehensive and rapid molecular profiling for brain tumors, which could contribute to clinical practices and research in the field of brain tumors.
Downregulated the expression of DNMT3B by suppressing PI3K/Akt signaling pathway and enhances the chemosensitivity of glioblastoma to temozolomide.
Authors: Kan Wenwu , Gao Linhui , Chen Jingnan , Chen Li , Zhang Guojun , Hao Bilie , He Min , Chen Xudong , wang cheng 
Published: October 2023

Abstract
Purpose
Glioblastoma is the most common malignant brain tumors and has the poorest prognosis. And a poor prognosis is attributed to chemoresistance to temozolomide (TMZ), the first-line drug for treating glioblastoma. This study aimed to investigate how to enhance the chemosensitivity of glioblastoma to temozolomide.
Methods
Human glioblastoma cell line U251 was used to established temozolomide-resistant U251 (U251-TMZ) cell line by stepwise induction of temozolomide-resistant strains. Reverse Transcription-quantitative PCR(RT-PCR) was applied to detect chemoresistance-related gene expression. Following DNMT3B-siRNA lentiviral vectors transfection and suppressing PI3K/Akt signaling pathway, western Blotting (WB) and RT-PCR were applied to detect DNMT3B gene, p-Akt, t-Akt, p-PI3K, t-PI3K protein expression and cell apoptosis was detect flow cytometry analyses.
Results
Whole-transcriptome analysis revealed that the level of DNMT3B gene expression was significantly up-regulated in U251-TMZ cell line compared to U251 cell line. Moreover, we found that DNMT3B down-expression is correlated with increasing the chemosensitivity of glioblastoma cells to TMZ. Meanwhile, we also found that p-Akt and p-PI3K protein expression in U251-TMZ cells were significant elevated compared with U251 cells. Subsequently, the PI3K/Akt signaling pathway was suppressed using LY294002, leading to a notable inhibition of PI3K phosphorylation and a significant decrease in DNMT3B expression in U251-TMZ cells.
Conclusion
DNMT3B down-expression can inhibit the proliferation of glioblastoma cells and induce the glioblastoma cells apoptosis in vitro. Additionally, the PI3K/Akt signaling pathway plays an important role in the chemosensitivity of glioblastoma cells to temozolomide by regulating the DNMT3B expression.
Determination of heavy metals Cd (II) and Pb (II) with an electrochemically treated pencil graphite electrode in carbonated mineral water using anodic stripping voltammetry
Authors: Afacan Reyhan Selin Uysal 
Published: October 2023

Abstract
The starting point of this study was to propose a simple and rapid electrochemical approach for quantitative analysis of heavy metal ions in natural mineral water samples. For this purpose, it was aimed to quantitatively determine Cd (II) and Pb (II) ions in gaseous mineral waters by square wave anodic stripping voltammetry (SWASV) by modification of pencil graphite electrode (PGE) with graphene oxide (GO) and deposition of auric acid solution. A three-step modification process was implemented to the PGE surface, including graphene oxide coating (GO/PGE), GO reduction (ERGO/PGE), and gold coating (Au-ERGO/PGE). The morphologies and electrochemical properties of the developed electrode were checked with scanning electron microscopy (SEM), cyclic voltammetry (CV), and SWASV. The results show the synergistic effect of graphene oxide and auric acid-activated surface and good electrical conductivity leading to the deposition of Cd (II) and Pb (II) on the electrode surface. Under optimum conditions, a linear behavior was obtained between currents and Cd (II) and Pb (II) concentrations in the range of 0.6–1.6 µM and 0.4–1.6 µM. Detection limits were calculated as 0.36 µM and 0.24 µM for Cd (II) and Pb (II), respectively. Finally, the improved electrode was applied for the detection of Cd (II) and Pb (II) in gaseous natural mineral water samples with high recovery rates. This study revealed that Au-ERGO/PGE modification could be an alternative route in practical applications of electrochemical detection of heavy metal ions.
WITHDRAWN: Seismic Imaging of the Kumaon-Garhwal Himalaya: Insights into the Main Himalayan Thrust and the Crust-Mantle Boundary
Authors: Mandal Prantik , Raghavan R. V. , Saha Satish , Biswas Rahul , Prathigadapa Raju 
Published: October 2023

Abstract

The seismic velocity tomography of reveals a high-velocity (5–20% increase in dVp, 4–16% increase in dVs, low Vp/Vs (~ 1.5–1.8)) upper crust between 0 and 20 km depth and a low-velocity (10–20% drop in dVp, 4–12% drop in dVs, high Vp/Vs (~ 1.65-2.0)) lower crust between 20 and 40 km depth. Our modelling detects a low-angle north-dipping low-velocity layer (10–20% drop in Vp and Vs, 10–15% increase in Vp/Vs) at depths of 8–20 km, which may be the Main Himalayan Thrust (MHT). The presence of low-velocity pockets in the lower crust may be indicative of partial melts, while those in the upper and middle crust may be indicative of aqueous or metamorphic fluids. The Common Conversion Point stacking (CCP) of radial P- Receiver Functions (PRFs) detects a double Moho structure below the rupture zone of the 1803 Mw7.8 Garhwal, the 1991 Mw6.8 Uttarkashi, and the 1999 Mw6.5 Chamoli earthquakes, which might be accumulating marked crustal stresses on the MHT. Our modelling also suggests that fluids or high pore-fluid pressure caused the above-mentioned three Mw≥6.5 earthquakes, which occurred in low-velocity zones on the MHT. Our CCP imaging also suggests a north-dipping crust-mantle boundary (with larger positive impedance contrast) at depths of 30–55 km while these CCP images also detect a north-dipping zone with larger negative impedance contrast at depths of 8–20 km, possibly representing the low-velocity MHT. Our joint inversion of radial PRFs and group velocity dispersion data of Rayleigh waves has also mapped an MHT and Moho that dip to the north.
Research on Injury Protection for Rear Pregnant Occupants in Frontal Impact
Authors: Wang Ping , Tang Youming , Zhang Yi , Mao Anli 
Published: October 2023

Abstract

This paper investigates the protective effects of restraint systems, specifically the three-point and combined safety belts, on pregnant occupants during transportation safety. The goal is to explore potential injury risks and enhance safety measures. Firstly, a restraint system model of the rear female occupant in the frontal full-width collision is established and validated. Secondly, the finite element model of the uterus at 30 weeks of pregnancy is established and coupled with Hybrid III 5th female facet human model to build a pregnant occupant model. Finally, according to the injury mechanism of the pregnant occupant and fetus, a comprehensive injury index WICp is proposed to assess the injury of the pregnant occupant and fetus. Research has been conducted on the effectiveness of wearing both a combined safety belt and a three-point safety belt by the pregnant occupant under various impact loads to ensure optimal protection. The results reveal that compared with the three-point safety belt, the combined safety belt worn by the pregnant occupant could reduce the abdominal compression value by 38% on average, decrease the Uterus Placental Interface (UPI) strain at the uteroplacental intersection by an average of 18.3%, and make the average reduction of the comprehensive injury by 13.4%. When the impact speed is less than 55km/h, the UPI strain value of the uteroplacental interface will not exceed the limit value of 60%. As a result, the risk of placental abruption for the pregnant occupant is reduced, and the protective effect during transportation safety for the pregnant and fetus is enhanced. The research results provide a scientific theoretical basis and reference value for pregnant occupants and fetuses to develop safer, more reliable, and more comfortable seat belts.
A Repertoire of Ethanol-Producing Human Microbes
Authors: MBAYE Babacar , WASFY Reham MAGDY , ALOU Maryam TIDJANI , BORENTAIN Patrick , GEROLAMI Rene , DUFOUR Jeans Charles , MILLION Matthieu 
Published: October 2023

Abstract

Background Studies of the relationship between the human microbiota and the host, particularly in the maintenance of health and the onset of disease, is still a topical issue. The microbiota is home to a number of microorganisms, including bacteria, yeasts, viruses and archaea, which play a crucial role in the body's proper functioning. In the presence of risk factors, mainly antibiotics, unbalanced diet and so on, a microbiota disequilibrium known as dysbiosis can set in, eventually leading to the onset of several diseases. Several mechanisms have been described for this dysbiosis, but the one linked to endogenous ethanol production is under-investigated. Results The aim of this review is to provide an inventory of all ethanol-producing microorganisms in humans. Over 36,0000,000 articles were searched on pubmed with our query, of which 7275 were retained. Of these, 5397 contributed to the review, enabling us to identify 85 ethanol-producing microorganisms in humans, including 61 bacterial species and 24 yeasts. Conclusion Our results establish an important role for endogenous ethanol in the onset of certain diseases. They will also enable us to better understand the origins of diseases linked to this mechanism, and to improve diagnosis and therapeutic management in the future.
Analysing the psychometric properties of the PHQ-9 using item response theory in a Chinese adolescent population
Authors: Gao Xuliang , Liu Ziyu 
Published: October 2023

Abstract
Background
People are more likely to fall victim to depression during adolescence since it is a period of rapid bio-psycho-social transformation. Despite this, most depression research has concentrated on clinical issues, and evaluating depressive symptoms in teenagers is not as widespread. This study used item response theory (IRT) to examine the psychometric properties of the Patient Health Report scale (PHQ-9) in Chinese adolescents. Meanwhile, item function difference tests were revealed to check whether there were differences in depression symptoms in this group based on education and gender.
Methods
In this research, the PHQ-9 was employed as a measurement tool, and 5958 valid data were obtained from 12 secondary schools in China (Mage= 13.484; SDage= 1.627; range 11–19 years; 52.17% boys).
Results
IRT examines that all items of PHQ-9 satisfy monotonicity, unidimensionality, local independence, and good psychometric properties. Furthermore, DIF analysis revealed gender and educational disparities in adolescent depressive symptoms.
Conclusion
The study indicates that the PHQ-9 possesses favorable psychometric properties in Chinese adolescents. As a result, it serves as a valuable tool for effectively screening depressive symptoms in adolescents. It provides a foundation for prioritizing the development of secondary school students' physical and mental health.
Short-Term Safety Evaluation of Albumin-Bound Paclitaxel in Intraoperative and Postoperative Hyperthermic Intraperitoneal Chemotherapy for Gastric Cancer
Authors: Zhou Jingxiang , Cai Xiaopeng , Lu Zhao , Xiong Bin , Peng Chunwei 
Published: October 2023

Abstract
Purpose
To evaluate the short-term safety of albumin-bound paclitaxel in hyperthermic intraperitoneal chemotherapy (HIPEC) during and after gastric cancer (GC) surgery.
Methods
A retrospective analysis of clinical data was conducted for GC surgery patients at Zhongnan Hospital of Wuhan University, from January 2020 to September 2022. The study group (n = 120) received HIPEC and the control group (n = 268) did not receive albumin-bound paclitaxel. Short-term safety indicators including intraoperative complications, hematological toxicity, liver and kidney function, and gastrointestinal function recovery, were compared between the two groups.
Results
There were no statistically significant differences between the two groups regarding intraoperative complications, hematological toxicity, liver and kidney function, and gastrointestinal function recovery time (P > 0.05 for all). In the study group, patients were further divided into subgroups based on dose and timing. Subgroup analysis revealed no significant differences among the different dose subgroups. However, when focusing on timing subgroups, postoperative subgroup exhibited significantly higher white blood cell counts and bilirubin levels compared to intraoperative subgroup, while the intraoperative subgroup had significantly higher bilirubin levels compared to both postoperative and intraoperative plus postoperative subgroups.
Conclusion
Albumin-bound paclitaxel demonstrates good safety and tolerability in HIPEC during and after GC surgery, without increasing the risk of intraoperative complications.
The effect of remote programming on deep brain stimulation in Parkinson’s disease: a retrospective study
Authors: Li Dianyou , Wan Xiaonan , Duan Chengcheng , Lin Zhengyu , Zeng Zhitong , Zhang Chencheng 
Published: October 2023

Abstract
For Parkinson’s disease (PD) patients undergoing deep brain stimulation (DBS), remote programming (RP) was developed to eliminate the burden of repeat office visits for standard programming (SP) sessions to maximize the treatment effect. No studies comparing the treatment effects of RP with SP directly were performed. Thirty-two propensity score matched patients were retrospectively recruited and allocated to the RP or SP group according to their programming methods, and clinical outcomes were compared at ~12-month follow-ups between the two groups. No differences were found in changes of the Unified PD Rating Scale III score, PD Questionnaire-8 score, or levodopa equivalent daily dose between the groups (p>0.05); and the average cost of the RP group decreased compared with their SP sessions (p<0.001), and was less than the average cost of the SP group (p=0.040), even though the patients in the RP group reside further from our center than patients in the SP group (p=0.004). This study demonstrated RP alleviates the cost burden of the short-term follow-ups for PD patients without compromising the DBS treatment effect.