bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ropivacaine inhibits the malignant behavior of lung cancer cells by regulating retinoblastoma-binding protein 4
Authors: Jia Weiai , Shen Junmei , Wei Sisi , Li Chao , Shi Jingpu , Zhao Lianmei , Jia Huiqun 
Published: November 2023

Background
Ropivacaine is a local anesthetic commonly used in regional nerve blocks to manage perioperative pain during lung cancer surgery. Recently, the antitumor potential of ropivacaine has received considerable attention. Our previous study showed that ropivacaine treatment inhibits the malignant behavior of lung cancer cells in vitro. However, the potential targets of ropivacaine in lung cancer cells have not yet been fully identified. This study aimed to explore the antitumor effects and mechanisms of action of ropivacaine in lung cancer.

Methods
Lung cancer A549 cells were treated with or without 1 mM ropivacaine for 48 h. Quantitative proteomics was performed to identify the differentially expressed proteins (DEPs) triggered by ropivacaine treatment. STRING and Cytoscape were used to construct protein-protein interaction (PPI) networks and analyze the most significant hub genes. Overexpression plasmids and small interfering RNA were used to modulate the expression of key DEPs in A549 and H1299 cells. MTS, transwell assays, and flow cytometry were performed to determine whether the key DEPs were closely related to the anticancer effect of ropivacaine on the malignant behavior of A549 and H1299 cells.

Results
Quantitative proteomic analysis identified 327 DEPs (185 upregulated and 142 downregulated proteins) following ropivacaine treatment. Retinoblastoma-binding protein 4 (RBBP4) was one of the downregulated DEPs and was selected as the hub protein. TCGA database showed that RBBP4 was significantly upregulated in lung cancer and was associated with poor patient prognosis. Inhibition of RBBP4 by siRNA resulted in a significant decrease in the proliferation and invasive capacity of lung cancer cells and the induction of cell cycle arrest. Additionally, the results indicated RBBP4 knockdown enhanced antitumor effect of ropivacaine on A549 and H1299 cells. Conversely, the overexpression of RBBP4 using plasmids reversed the inhibitory effects of ropivacaine.

Conclusion
Our data suggest that ropivacaine suppresses lung cancer cell malignancy by downregulating RBBP4 protein expression, which may help clarify the mechanisms underlying the antitumor effects of ropivacaine.
Context of substance initiation among urban Native Americans: an exploratory retrospective case-control study
Authors: Guenzel Nicholas , Daisy Dai Hongying , Dean Lyndsay 
Published: November 2023

Background
Addiction is a significant problem among many Native American groups but has rarely been examined in urban populations. In particular, little is known about the context in which urban Native Americans first use substances. This study compares cases (people with a history of addiction) to controls (people without a history of addiction) on demographics, substance use history, context of first substance use, and polysubstance use. In addition, this appears to be the first study to overcome the lack of Native American professionals by employing and training lay community members to identify criteria of substance use disorders in survey participants. Employing community members helped foster trust that enabled the revelation of sensitive and often illegal activity. As a result, the investigators were able to recruit participants who likely would not have engaged with traditional researchers.

Methods
The trained Native American lay research assistants recruited community members and administered surveys. They first asked questions regarding the criteria for substance use disorders. Individuals who were determined to have met criteria for a substance use disorder in the past were classified as cases (n = 38) and those who never met such criteria were classified as controls (n = 42). They then asked demographic, substance use, and polysubstance use questions. Lastly, eight cases and eight controls were randomly selected for a second interview by a licensed drug and alcohol counselor (LDAC) who conducted a blinded assessment regarding the presence or absence of a history of a substance use disorder.

Results
Both groups reported a relatively young age of first substance use (age 16 years for cases and age 15 years for controls). Alcohol was the first substance most commonly used in both groups. Controls reported first benzodiazepine use at a younger age than cases but no other significant differences were found. Both groups reported first obtaining their first drug from family, friends, or at home (rather than a party, bar, or store). Most commonly, the location of their first use of drugs occurred at a friend’s home, a party, a bar, or school rather than at their own home. Cases were marginally more likely to report that their first drug use occurred with a friend rather than with a family member when compared with controls. The majority of both groups reported that their first drug use occurred with other Native Americans rather than with non-Native Americans. Polysubstance use was common in both groups (43–45%). There were no significant differences between the groups regarding polysubstance use. The LDAC arrived at the same determination as the trained research assistants on all eight cases and eight controls.
Assessment of Sexual Maturity and Spawning Biology of Channa punctata (Bloch, 1793): Conservation and Reproduction
Authors: Awal Md. Rabiul , Rahman Md. Ashikur , Khanoma Maliha , Islam Md. Saiful , Bhadra Anuradha , Mahmud Yahia 
Published: November 2023

The study was conducted in the freshwater station of the Bangladesh Fisheries Research Institute in Mymensingh between September 2021 and August 2022. Finding the sex ratio, sexual maturity, LWR, reproductive biology, fecundity, and histological examination of gonads of C. punctata were the main objectives of the present study. The correlation between length and weight was determined by analyzing the power function. Both sexes showed positive allometric growth and the correlation between length and weight was TW= 0.011TL3.09 for males and TW= 0.009TL3.11 for females. The mean condition factor for males was 1.09 (0.89 to 1.24), whereas the mean condition factor for females was 1.12 (0.95 to 1.25). In both cases, the condition factor was more than 1, indicating that the physical condition of both sexes was excellent. Among the 329 samples, 142 (43.16%) were male and 187 (56.84%) were female (male: female = 1: 1.32). The chi-square test showed a significant departure from the 1:1 ratio between the number of males and females within the sample months (df = 1, χ2= 0.01, p=0.05). When they reached first sexual maturity (L50), both sexes were of similar size, as evidenced by their lengths of 14.95 cm. The peak spawning season for both sexes was observed in June and July; however spawning season lasted from April to July. According to the study results, July was recorded the highest absolute fecundity (23609), while December recorded the lowest (1998).
Prevalence of Work-Related Stress and its Associated Factors among Nursing Staff in King Abdallah Complex at Jeddah City
Authors: Al-Shehri Maryam Mohammed , Harazi Nouran , Alhassani Sheikah , Alshahrani Waleed Abdullah , Almzmommi Fawziah , Almalki Ohoud Saad , Qusti Shahad Numan 
Published: November 2023

Aim: This study aims to assess the prevalence of work-related stress and its impact on nurses’ work performance at King Abdullah Complex in Jeddah City.
Methods: A cross-sectional- study was conducted using a previously validated questionnaire distributed among nursing staff at King Abdullah Complex in Jeddah City.
Duration of the Study: This study was conducted for some time from September 2022 until August 2023.
Results: From a total of 263 nursing participants, the study findings reported that the prevalence of Work-related stress among nursing staff in the King Abdallah Complex at Jeddah City was 63.7% and most of the nurses feel that their job negatively impacts their physical or emotional health. Many nurses expressed that they have insufficient recognition or rewards for good performance at work.
Conclusion: The findings of the study indicate that work-related stress is a pervasive concern among the nursing staff employed at King Abdullah Complex. A considerable percentage of nursing workers have reported encountering different levels of stress within their everyday work settings.
LIDERANÇA FEMININA NA GESTÃO PÚBLICA: UM ESTUDO DE CASO NA UNIVERSIDADE DA INTEGRAÇÃO INTERNACIONAL DA LUSOFONIA AFRO-BRASILEIRA (UNILAB)
Authors: Costa Neila Priscila , Gonçalves Lenira Mendes Monteiro 
Published: November 2023

RESUMO
O presente trabalho investigou e discutiu sobre gênero e gestão pública a partir da temática da liderança feminina em uma universidade pública, a Universidade da Integração Internacional da Lusofonia Afro-brasileira, campus dos Malês, localizada no município baiano de São Francisco do Conde. A pesquisa é qualitativa, ancorada em revisão bibliográfica e em entrevista estruturada. Uma professora que exerce função de diretora no referido campus foi entrevistada, configurando um estudo de caso. Objetivou-se compreender, através de seu relato/narrativa, como acontece a (sub-)representação das mulheres na gestão pública. Para o momento da análise dos dados gerados em entrevista, foram consideradas como categorias analíticas, teto de vidro (labirinto de cristal), também conhecida como segregação vertical, bem como a segregação horizontal. A participante nos convida a pensar que as mulheres estão aptas a exercer cargos de liderança, ainda que a realidade social obstaculize essa empreitada.

Palavras-chave: Gênero. Gestão Pública. Liderança Feminina. Instituição de Ensino.
Gel‐Staining Techniques – Dyeing to Know It All
Authors: Mansour née Gauci Victoria J , Noaman Nour , Coorssen Jens R 
Published: November 2023

Abstract

Staining techniques are the primary method for quantitative detection of gel‐resolved proteins. With stain sensitivity defining the extent of total protein detected, staining‐technique enhancements that facilitate increased detection of low‐abundance proteins are constantly sought. Although stains must exhibit high detection sensitivity, they must also be broadly applicable, practical, cost‐effective and compatible with downstream identification techniques. Currently, the most widely utilised in‐gel protein stains are colloidal Coomassie Brilliant Blue and SYPRO Ruby (or slight variants on each). As with any stain, each requires extensive characterisation and rigorous optimisation of protocols to effectively define limits of sensitivity and quantitative capacity. However, the procedures involved in preparing, resolving, imaging and analysing samples can also have significant consequences on quantitative outcomes, and thus must be fully considered in order to facilitate detailed and reproducible analyses (i.e. as required in top‐down proteomics).
Clinical and imaging predictors of late‐onset GM2 gangliosidosis: A scoping review
Authors: Godbole Neha P. , Haxton Elizabeth , Rowe Olivia E. , Locascio Joseph J. , Schmahmann Jeremy D. , Eichler Florian S. , Ratai Eva‐Maria , Stephen Christopher D. 
Published: November 2023

AbstractObjectiveLate‐onset GM2 gangliosidosis (LOGG) subtypes late‐onset Tay‐Sachs (LOTS) and Sandhoff disease (LOSD) are ultra‐rare neurodegenerative lysosomal storage disorders presenting with weakness, ataxia, and neuropsychiatric symptoms. Previous studies considered LOTS and LOSD clinically indistinguishable; recent studies have challenged this. We performed a scoping review to ascertain whether imaging and clinical features may differentiate these diseases.MethodsWe examined MEDLINE/non‐MEDLINE databases up to May 2022. Articles reporting brain imaging findings in genetically/enzymatically confirmed LOGG, symptom onset at age ≥ 10 years (or evaluated at least once ≥18 years) were included, yielding 170 LOGG patients (LOTS = 127, LOSD = 43) across 68 papers. We compared LOTS versus LOSD and performed regression analyses. Results were corrected for multiple comparisons.ResultsAge of onset was lower in LOTS versus LOSD (17.9 ± 8.2 vs. 23.9 ± 14.4 years, p = 0.017), although disease duration was similar (p = 0.34). LOTS more commonly had psychosis/bipolar symptoms (35.0% vs. 9.30%, p = 0.011) but less frequent swallowing problems (4.10% vs. 18.60%, p = 0.041). Cerebellar atrophy was more common in LOTS (89.0%) versus LOSD (60.5%), p < 0.0001, with more severe atrophy in LOTS (p = 0.0005). Brainstem atrophy was documented only in LOTS (14.2%). Independent predictors of LOTS versus LOSD (odds ratio [95% confidence interval]) included the presence of psychosis/bipolar symptoms (4.95 [1.59–19.52], p = 0.011), no swallowing symptoms (0.16 [0.036–0.64], p = 0.011), and cerebellar atrophy (5.81 [2.10–17.08], p = 0.0009). Lower age of onset (0.96 [0.93–1.00], p = 0.075) and tremor (2.50 [0.94–7.43], p = 0.078) were marginally statistically significant but felt relevant to include in the model.InterpretationThese data suggest significant differences in symptomatology, disease course, and imaging findings between LOTS and LOSD.
Long‐Life Lithium‐Metal Batteries with an Ultra‐High‐Nickel Cathode and Electrolytes with Bi‐Anion Activity
Authors: Zhang Xianhui , Cui Zehao , Manthiram Arumugam 
Published: November 2023

AbstractAnion chemistry in electrolytes can greatly dictate the nature and quality of passivation layers on both cathode and anode surfaces. This will be more significant when it comes to highly reactive Li‐metal anode and aggressive high‐nickel cathodes. Herein, a competitive bi‐anion activity is found in electrolytes with the co‐existence of two anions, which leads to a controlled Li‐salt decomposition kinetics and entirely favorable interphasial chemistry on both Li‐metal anode and ultrahigh‐nickel cathode. The proposed bi‐anion localized high‐concentration electrolytes are demonstrated to exhibit superior electrochemical compatibility toward Li metal and long‐term cycling stabilities under both 4.4 and 4.6 V in Li‐metal batteries with ultrahigh‐nickel cathode. This study sheds fresh light on dendrite‐free Li‐metal anodes and provides guidance to achieve high‐energy‐density batteries.
A Small‐Molecule Polycationic Crosslinker Boosts Alginate‐Based Bioinks for Extrusion Bioprinting
Authors: Gao Chongjian , Tang Lan , Qu Huawei , Wu Mingming , Zhou Tian , Wen Chunyi , Wang Pinpin , Xu Nan , Ruan Changshun 
Published: November 2023

AbstractThe importance of bioink suitability for extrusion bioprinting tissue‐like constructs cannot be overemphasized. Owing to their excellent accessibility, alginate (Alg) ‐based bioinks are widely used. However, the printability accuracy and post‐printing stability of current Alg‐based bioinks remain challenging, especially for large‐size fabrication. Herein, an improvement strategy for pre‐crosslinking and post‐crosslinking of methacrylated Alg (AlgMA) bioinks in extrusion bioprinting is presented via introduction of methacrylated ɛ‐polylysine (ɛ‐PLMA) as a small‐molecule polycationic crosslinker. Due to their electrostatic interaction and covalent bonding, ɛ‐PLMA significantly reinforce the operability and prolong stability of AlgMA bioinks, in comparison with single‐molecule Ca2+ or large‐molecule methacrylated gelatin as pre‐crosslinkers. Meanwhile, attributed to their regulation of a charged microenvironment (−345.25 to 121.55 mV) and hydrophilicity (26.64° to 52.00°), the cells in bioprinted AlgMA/PLMA constructs demonstrated improved viability and vitality. Therefore, such a strategy of introducing small‐molecule cationic crosslinker ɛ‐PLMA is a promising booster for improving Alg‐based extrusion bioprinting, potential to extend its biomedical applications.
Raman Spectroscopy Application in Anisotropic 2D Materials
Authors: Zhao Xun , Li Zhipu , Wu Shiru , Lu Min , Xie Xiaoji , Zhan Da , Yan Jiaxu 
Published: November 2023

AbstractIn recent years, anisotropic 2D materials (black phosphorus, ReS2, WTe2, etc.) have garnered significant attention due to their orientation‐dependent physical and chemical properties, along with their potential applications in micro‐nano device development, such as crystal‐based diodes, polarized light photodetectors, and directional heat transfer. As a practical, quick, and nondestructive characterization tool, Raman spectroscopy, with its unique and unmatched advantages in studying anisotropic materials, plays a crucial role. It enables lattice orientation identification, investigation of structural phase transitions, and examination of anisotropic lattice vibrations, among other aspects. Here, a comprehensive review of recent developments in Raman spectroscopy research on anisotropic materials is provided. To begin, this study introduces the classification of anisotropic materials before delving into the polarized Raman spectroscopy principle. Various research directions of Raman spectroscopy in anisotropic materials are explored, including lattice orientation identification, temperature dependence, interlayer coupling, electron–phonon interaction, thickness dependence, and high‐pressure phase transition. Finally, potential future directions in the field of Raman spectroscopy for anisotropic materials are discussed, and the potential challenges that may arise are addressed.
Lithium Dredging and Capturing Dual‐Gradient Framework Enabling Step‐Packed Deposition for Dendrite‐Free Lithium Metal Anodes
Authors: Pan Jiajie , Shi Kaixiang , Wu Hao , Li Junhao , Zhang Rui , Liu Quanbing , Liang Zhenxing 
Published: November 2023

AbstractDue to the sterile lithium affinity and poor Li+ regulation ability, lithium metal tends to deposit on the top of substrate materials, which lowers the space utilization and deteriorates the dendrites growth, further increasing the risk of short circuits. It is necessary to guide lithium deposition and adjust Li+ flux at the base of substrate toward alleviating those issues of lithium metal anodes (LMAs). Herein, a dredging and capturing dual‐gradient framework is conveniently fabricated toward inducing the Li+ migration and deposition. The dual‐gradient framework combined with upper Ni3S2 nanowires with lower Li+ migration barrier and bottom Ni2P nanowires with stronger Li affinity, such reasonable distribution of components conquers the ionic concentration gradient to acquire a “step‐packed” lithium deposition mode. Moreover, the in situ formation of Li2S/Li3P‐enriched SEI further promotes Li+ transport to the interior framework. Consequently, a high average Coulombic efficiency of 98% over 230 cycles and a lifespan of 180 h (10 mA cm−2, 5 mAh cm−2) are achieved. The LiFePO4 full cells also exhibit a considerable capacity retention. This work provides a feasible strategy of step‐packing lithium deposition for significant framework design insight to boost practical lithium metal batteries (LMBs) development.
Extending Ring‐Chain Coupling Empirical Law to Lithium‐Mediated Electrochemical Ammonia Synthesis
Authors: Li Ya , Wang Zhenkang , Ji Haoqing , Wang Mengfan , Qian Tao , Yan Chenglin , Lu Jianmei 
Published: November 2023

AbstractWith its efficient nitrogen fixation kinetics, electrochemical lithium‐mediated nitrogen reduction reaction (LMNRR) holds promise for replacing Haber–Bosch process and realizing sustainable and green ammonia production. However, the general interface problem in lithium electrochemistry seriously impedes the further enhancement of LMNRR performance. Inspired by the development history of lithium battery electrolytes, here, we extend the ring‐chain solvents coupling law to LMNRR system to rationally optimize the interface during the reaction process, achieving nearly a two‐fold Faradaic efficiency up to 54.78±1.60 %. Systematic theoretical simulations and experimental analysis jointly decipher that the anion‐rich Li+ solvation structure derived from ring tetrahydrofuran coupling with chain ether successfully suppresses the excessive passivation of electrolyte decomposition at the reaction interface, thus promoting the mass transfer of active species and enhancing the nitrogen fixation kinetics. This work offers a progressive insight into the electrolyte design of LMNRR system.
Does motivation for practising mindfulness moderate the effects of mindfulness training on stressed adults?
Authors: Moran Megan J. , Miller Reagan L. , Rzonca Addie , Krause Jill , Adams Melanie S. , Rigsby Brock A. , Prince Mark A. , Lucas‐Thompson Rachel G. 
Published: November 2023

AbstractBackgroundMindfulness‐based intervention (MBI) has been associated with positive health outcomes in adults; however, there are important gaps in our knowledge of factors that influence which individuals are more likely to engage with and benefit from these interventions. Research on other types of behavioural interventions suggests that motivation changes over the course of intervention and may moderate attendance and therapeutic outcomes. This study investigated changes in motivation for practising mindfulness across an MBI and associations between baseline motivation and outcomes, to gain a preliminary understanding of the role of motivation in MBI adherence and outcomes.MethodsIn a sample of adults (N = 80) who received an MBI, we characterised change in motivation from pre‐ to postintervention and tested whether baseline motivation predicted change in attendance and outcomes.ResultsWe found that some, but not all, dimensions of motivation for practising mindfulness increased and also were associated with attendance and pre–post change stress and emotion regulation.ConclusionsOur findings suggest that there may be potential for optimising MBI through attention to individual differences in motivation and change in motivation over time. The findings justify research with larger sample sizes, control groups and repeated measurements to test the temporal ordering of changes in these outcomes.
Exploring Thermomyces lanuginosus Lipase (TLL)‐PdNPs Nanohybrid as Suitable Catalyst for One‐pot Synthesis of Bis(3‐indolyl)phenylmethane
Authors: Santos A. Sofia , Losada‐Garcia Noelia , Garcia‐Sanz Carla , Silva Artur M. S. , Marques M. Manuel B. , Palomo Jose M. 
Published: November 2023

AbstractPalladium nanohybrids were synthesized and applied to the one‐pot synthesis of bis(3‐indolyl)methanes by selective C−C bond reaction from benzyl alcohol and indole. A T. lanuginosus lipase‐palladium nanoparticles hybrid (Pd@TLL) was synthesized, yielding PdNPs with an average diameter size of 5 nm. This heterogeneous catalyst was first tested in the selective oxidation of benzyl alcohol to benzaldehyde in different solvents. Then, the direct formation of bis(3‐indolyl)methane, by in situ oxidation and C−C coupling, was successfully evaluated under different conditions, obtaining >99 % conversion at 80 °C in toluene, with a TOF value of 9 min−1 and 89 % in pure water, demonstrating the versatility of these biohybrids.