bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical, biochemical and molecular characterization of a new case with FDX2‐related mitochondrial disorder: Potential biomarkers and treatment options
Authors: Wongkittichote Parith , Pantano Cassandra , He Miao , Hong Xinying , Demczko Matthew M. 
Published: January 2024

AbstractFerredoxin‐2 (FDX2) is an electron transport protein required for iron–sulfur clusters biosynthesis. Pathogenic variants in FDX2 have been associated with autosomal recessive FDX2‐related disorder characterized by mitochondrial myopathy with or without optic atrophy and leukoencephalopathy. We described a new case harboring compound heterozygous variants in FDX2 who presented with recurrent rhabdomyolysis with severe episodes affecting respiratory muscle. Biochemical analysis of the patients revealed hyperexcretion of 2‐hydroxyadipic acid, along with previously reported biochemical abnormalities. The proband demonstrated increased lactate and creatine kinase (CK) with increased amount of glucose infusion. Lactate and CK drastically decreased when parenteral nutrition containing high protein and lipid contents with low glucose was initiated. Overall, we described a new case of FDX2‐related disorder and compare clinical, biochemical and molecular findings with previously reported cases. We demonstrated that 2‐hydroxyadipic acid biomarker could be used as an adjunct biomarker for FDX2‐related disorder and the use of parenteral nutrition as a treatment option for the patient with FDX2‐related disorder during rhabdomyolysis episode.Highlights2‐Hydroxyadipic acid can serve as a potential adjunct biomarker for iron‐sulfur assembly defects and lipoic acid biosynthesis disorders. Parenteral nutrition containing high lipid and protein content could be used to reverse acute rhabdomyolysis episodes in the patients with FDX2‐related disorder.
Study on the epidemiological characteristics of enterovirus among pediatric patients in Hangzhou, China: A comparison between the pre‐COVID‐19, COVID‐19 pandemic, and post‐COVID‐19 periods
Authors: Sun Yanhong , Zhou Jianming , Nie Wenjian , Tian Dandan , Ye Qing 
Published: January 2024

AbstractNonpharmaceutical interventions (NPIs) for coronavirus disease 2019 (COVID‐19) not only reduce the prevalence of this disease among children but also influence the transmission of other viruses. This retrospective study investigated the impact of NPIs on human enterovirus (HEV) infection in children diagnosed with hand, foot, and mouth disease (HFMD) or herpangina (HA) in Hangzhou, China. We collected and analyzed the laboratory results and clinical data of children diagnosed with HFMD or HA during the following periods: pre‐COVID‐19 (January 2019 to December 2019), the COVID‐19 pandemic (January 2020 to December 2022), and post‐COVID‐19 (January to December 2023). A total of 41 742 specimens that met the inclusion criteria were obtained, of which 1998 (4.79%) tested positive for enterovirus. In comparison to those in the pre‐COVID‐19 period, which had 695 (5.63%) HEV‐positive specimens, the numbers dramatically decreased to 69 (1.19%), 398 (5.12%), and 112 (1.58%) in 2020, 2021, and 2022, respectively, but significantly increased to 724 (8.27%) in 2023. Seasonal peaks of infections occurred in May, June, July, and August each year, with the total detection rate ranging from 2019 to 2023 being 9.41% in May, 22.47% in June, 28.23% in July, and 12.16% in August, respectively. The difference in the detection rates of HEV infection between males and females was statistically significant (p < 0.005), with 5.11% (1221/23 898) of males and 4.35% (777/17 844) of females testing positive, resulting in a male‐to‐female positive ratio of 1.57:1. Among the age groups, 11.25% (378/3360) of the children aged 3–5 years had the highest detection rate, which steadily decreased with increasing or decreasing age. The detection of HEV indicated that >95% of the viruses were other types than the previously commonly reported enterovirus 71 (EV‐A71) and coxsackievirus A16 (CV‐A16). In conclusion, NPIs for COVID‐19 may be effective at reducing the transmission of HEV. However, with the relaxation of NPIs, the detection rate of HEVs increased slowly to a certain extent. Active awareness and surveillance of the epidemiological characteristics of HEV are essential for preventing, controlling, and managing the development of HFMD and HA, as well as contributing to the development of a multivalent HFMD vaccine.
Co‐augmentation of a transport gene mfsT1 in Mycolicibacterium neoaurum with genome engineering to enhance ergothioneine production
Authors: Ding Ya‐Xue , Chen Jun‐Wei , Ke Jie , Hu Fei‐Yang , Wen Jia‐Chen , Dong Yu‐Guo , Wang Feng‐Qing , Xiong Liang‐Bin 
Published: January 2024

AbstractErgothioneine (EGT) is a rare thiohistidine derivative with exceptional antioxidant properties. The blood level of EGT is considered highly reliable predictors for cardiovascular diseases and mortality, yet animals lack the ability to synthesize this compound. Free plasmids have been previously used to overexpress genes involved in the EGT biosynthetic pathway of Mycolicibacterium neoaurum. Here, we tentatively introduced a putative transporter gene mfsT1 into high‐copy plasmids and sharply increased the ratio of extracellular EGT concentration from 18.7% to 44.9%. Subsequently, an additional copy of egtABCDE, hisG, and mfsT1 was inserted into the genome with a site‐specific genomic integration tool of M. neoaurum, leading a 2.7 times increase in EGT production. Co‐enhancing the S‐adenosyl‐L‐methionine regeneration pathway, or alternatively, the integration of three copies of egtABCDE, hisG and mfsT1 into the genome further increased the total EGT yield by 16.1% (64.6 mg/L) and 21.7% (67.7 mg/L), respectively. After 168‐h cultivation, the highest titer reached 85.9 mg/L in the latter strain with three inserted copies. This study provided a solid foundation for genome engineering to increase the production of EGT in M. neoaurum.
Co‐metabolism of Na+/K+ ion regulated physiological enhancement on selenium‐accumulation in Saccharomyces yeasts
Authors: Hao Wenhui , Li Ying , Guo Hanlin , Chen Jian , Pi Fuwei 
Published: January 2024

AbstractBACKGROUNDSelenium is an important nutritional supplement that mainly exists naturally in soil as inorganic selenium. Saccharomyces cerevisiae cells are excellent medium for converting inorganic selenium in nature into organic selenium.RESULTSUnder the co‐stimulation of sodium selenite (Na2SeO3) and potassium selenite (K2SeO3), the activity of selenophosphate synthetase (SPS) was improved up to about five folds more than conventional Na2SeO3 group with the total selenite salts content of 30 mg/L. Transcriptome analysis first revealed that due to the sharing pathway between sodium ion (Na+) and potassium ion (K+), the K+ largely regulates the metabolisms of amino acid and glutathione under the accumulation of selenite salt. Furthermore, K+ could improve the tolerance performance and selenium‐biotransformation yields of Saccharomyces cerevisiae cells under Na2SeO3 salt stimulation.CONCLUSIONThe important role of K+ in regulating the intracellular selenium accumulation especially in terms of amino acid metabolism and glutathione, suggested a new direction for the development of selenium‐enrichment supplements with Saccharomyces cerevisiae cell factory. © 2024 Society of Chemical Industry.
Arithmeticity of the Kontsevich–Zorich monodromies of certain families of square‐tiled surfaces II
Authors: Kany Manuel , Matheus Carlos 
Published: January 2024

AbstractIn this note, we extend the scope of our previous work joint with Bonnafoux, Kattler, Niño, Sedano‐Mendoza, Valdez, and Weitze‐Schmithüsen by showing the arithmeticity of the Kontsevich–Zorich monodromies of infinite families of square‐tiled surfaces of genera four, five, and six.
On Fourier–Mukai type autoequivalences of Kuznetsov components of cubic threefolds
Authors: Liu Ziqi 
Published: January 2024

AbstractWe determine the group of all Fourier–Mukai type autoequivalences of Kuznetsov components of smooth complex cubic threefolds, and provide yet another proof for the Fourier–Mukai version of the categorical Torelli theorem for smooth complex cubic threefolds.
Polar Molecules Regulating the Regio‐ and Stereoselectivity of Polymerization of Conjugated Dienes Catalyzed by CGC‐Type Rare‐Earth Metal Catalysts
Authors: Fu Zhijie , Song Chuang , Chen Chongan , Qi Yuanxia , Pei Jingting , Chen Yuwei , Zheng Jie , Zhao Yanan , Luo Yi , Li Xiaofang 
Published: January 2024

AbstractHerein, a concise, effective, and scalable strategy is reported that the introduction of polar molecules (PMs) (e.g., anisole (PhOMe), phenetole (PhOEt), 2‐methoxynaphthalene (NaphOMe), thioanisole (PhSMe), and N,N‐dimethylaniline (PhNMe2)) as continuously coordinated neutral ligand of cationic active species in situ generated from the constrain‐geometry‐configuration–type rare‐earth metal complexes A–F/AliBu3/[Ph3C][B(C6F5)4] ternary systems can easily switch the regio‐ and stereoselectivity of the polymerization of conjugated dienes (CDs, including 2‐subsituted CDs such as isoprene (IP) and myrcene (MY), 1,2‐disubstituted CD ocimene (OC), and 1‐substituted polar CD 1‐(para‐methoxyphenyl)‐1,3‐butadiene (p‐MOPB)) from poor selectivities to high selectivities (for IP and MY: 3,4‐selectivity up to 99%; for OC: trans‐1,2‐selectivity up to 93% (mm up to 90%); for p‐MOPB: 3,4‐syndioselectivity (3,4‐ up to 99%, rrrr up to 96%)). DFT calculations explain the continuous coordination roles of PMs on the regulation of the regio‐ and stereoselectivity of the polymerization of CDs. In comparison with the traditional strategies, this strategy by adding some common PMs is easier and more convenient, decreasing the synthetic cost and complex operation of new metal catalyst and cocatalyst. Such regio‐ and stereoselective regulation method by using PMs is not reported for the coordination polymerization of olefins catalyzed by rare‐earth metal and early transition metal complexes.
Synthetically accessible de novo design using reaction vectors: Application to PARP1 inhibitors**
Authors: Ghiandoni Gian Marco , Flanagan Stuart R. , Bodkin Michael J. , Nizi Maria Giulia , Galera‐Prat Albert , Brai Annalaura , Chen Beining , Wallace James E. A. , Hristozov Dimitar , Webster James , Manfroni Giuseppe , Lehtiö Lari , Tabarrini Oriana , Gillet Valerie J. 
Published: January 2024

AbstractDe novo design has been a hotly pursued topic for many years. Most recent developments have involved the use of deep learning methods for generative molecular design. Despite increasing levels of algorithmic sophistication, the design of molecules that are synthetically accessible remains a major challenge. Reaction‐based de novo design takes a conceptually simpler approach and aims to address synthesisability directly by mimicking synthetic chemistry and driving structural transformations by known reactions that are applied in a stepwise manner. However, the use of a small number of hand‐coded transformations restricts the chemical space that can be accessed and there are few examples in the literature where molecules and their synthetic routes have been designed and executed successfully. Here we describe the application of reaction‐based de novo design to the design of synthetically accessible and biologically active compounds as proof‐of‐concept of our reaction vector‐based software. Reaction vectors are derived automatically from known reactions and allow access to a wide region of synthetically accessible chemical space. The design was aimed at producing molecules that are active against PARP1 and which have improved brain penetration properties compared to existing PARP1 inhibitors. We synthesised a selection of the designed molecules according to the provided synthetic routes and tested them experimentally. The results demonstrate that reaction vectors can be applied to the design of novel molecules of biological relevance that are also synthetically accessible.
Design of circularly polarized antenna with wide overlapped axial‐ratio and gain beamwidths for upper hemispherical coverage
Authors: Ren Junhao , Liu Hongmei , Yu Da , Zeng Youjie , Wang Zhongbao 
Published: January 2024

AbstractIn this letter, a circularly polarized antenna with wide overlapped axial ratio and gain beamwidth is proposed for upper hemispherical coverage. By bending half of the dipoles to the vertical direction, and connecting eight metal columns to the ground, the half‐power beamwidth (HPBW) of all cut planes can be increased effectively. On the basis, one reactive impedance structure is applied as the reflector to achieve unidirectional radiation, as well as increase the 3‐dB axial ratio beamwidth (ARBW) of the antenna. Miniaturized four‐feed networks are utilized to provide excitation, and the shielding technique is applied to reduce its influences on the antenna radiation performances. Measurements show that the proposed antenna can operate in the range of 1.51–1.59 GHz, which covers the upper band of global navigation satellite system (GNSS). Besides, at 1.561 GHz, the measured HPBWs and 3‐dB ARBWs are larger than 131° and 135° in all cut planes. The wide overlapped beamwidth of the proposed antenna with small size indicates the potential applications in high‐precision GNSS navigations.
A concept analysis of transitional care for people with cancer
Authors: Mardani Abbas , Azizi Maryam , Alazmani Noodeh Farshid , Alizadeh Azizeh , Maleki Maryam , Vaismoradi Mojtaba , Glarcher Manela 
Published: January 2024

AbstractAimTransitional care as the journey between different caregivers in multiple healthcare centres is crucial for the provision of care to people with cancer, but it is often complex and poorly coordinated. This study aimed to analyse the concept of transitional care for people with cancer.DesignRodgers' evolutionary concept analysis.MethodsA systematic literature search was conducted on the databases of PubMed (including MEDLINE), EMBASE, Scopus and Web of Science to retrieve articles published between 2000 and 2022.ResultsTwenty‐nine eligible articles were selected and their findings were classified in terms of related concepts and alternative terms, antecedents, attributes and consequences. Attributes included three main categories, namely ‘nurse‐related attributes’, ‘organisation‐related attributes’ and ‘patient‐related attributes’. Antecedents of transitional care for people with cancer were categorized into two main categories: ‘patient‐related antecedents’ and ‘caregiver‐related antecedents’. Consequences were categorized into ‘psychological consequences’ and ‘objective consequences’.
Psychosocial markers of pre‐hospital delay in patients with diabetic foot: A cross‐sectional survey
Authors: Xu Huiwen , Wu Chen , Xiang Shengxiao , Qiu Shuang , Chen Yan , Takashi En , Yanagihara Kiyoko , Xie Ping 
Published: January 2024

AbstractAimThis study aimed to determine the psychosocial markers associated with pre‐hospital delay among patients with diabetic foot (DF).DesignThis study has a cross‐sectional design.MethodsThe participants completed a questionnaire including pre‐hospital time, demographic characteristics, Social Support Rate Scale, Brief Illness Perception Questionnaire and Type D Personality Scale‐14. Bivariate and multivariate analyses were conducted to explore independent associations with pre‐hospital delay.ResultsOnly 1.8% (3/164) of participants arrived at the hospital for medical care in 24 h of symptom onset. Patients with low utilization of social support (p = 0.029), low negative illness perceptions (p = 0.014) and high levels of negative affectivity (p = 0.009) are likely to arrive late at the clinic. Medical staff should pay attention to identifying diabetic patients' Type D personalities and take actions to improve their social support as well as illness perception, so as to reduce the occurrence of hospital delay.Implications for the Profession and/or Patient CarePsychosocial factors play a vital role in the delay in seeking medical treatment for patients with DF. Medical staff need to improve patients' illness perception as well as self‐management ability through health education. Importantly, key family members provide an emotional and psychological support system for diabetic patients. Therefore, nurses need to work with family members together to give information and psychological support during family visits. Additionally, building and maintaining trust with patients is crucial to encouraging individuals to express their concerns and worries. In this case, nurses may identify patients' negative emotions and conduct timely intervention, so as to achieve favourable outcomes.Patient or Public ContributionThis study used a convenience sample of 164 participants with DF recruited from the wound clinic of Northern Jiangsu People's Hospital and Yangzhou Hospital of TCM in China.
Racial Disparities in Head and Neck Cancer: It's Not Just About Access
Authors: Whitehead Russell A. , Patel Evan A. , Liu Jeffrey C. , Bhayani Mihir K. 
Published: January 2024

AbstractObjectiveMedical literature identifies stark racial disparities in head and neck cancer (HNC) in the United States, primarily between non‐Hispanic white (NHW) and non‐Hispanic black (NHB) populations. The etiology of this disparity is often attributed to inequitable access to health care and socioeconomic status (SES). However, other contributors have been reported. We performed a systematic review to better understand the multifactorial landscape driving racial disparities in HNC.Data SourcesA systematic review was conducted in Covidence following Preferred Reporting Items for Systematic Reviews and Meta‐analyses Guidelines. A search of PubMed, SCOPUS, and CINAHL for literature published through November 2022 evaluating racial disparities in HNC identified 2309 publications.Review MethodsFull texts were screened by 2 authors independently, and inconsistencies were resolved by consensus. Three hundred forty publications were ultimately selected and categorized into themes including disparities in access/SES, treatment, lifestyle, and biology. Racial groups examined included NHB and NHW patients but also included Hispanic, Native American, and Asian/Pacific Islander patients to a lesser extent.ResultsOf the 340 articles, 192 focused on themes of access/SES, including access to high‐quality hospitals, insurance coverage, and transportation contributing to disparate HNC outcomes. Additional themes discussed in 148 articles included incongruities in surgical recommendations, tobacco/alcohol use, human papillomavirus‐associated malignancies, and race‐informed silencing of tumor suppressor genes.ConclusionDifferential access to care plays a significant role in racial disparities in HNC, disproportionately affecting NHB populations. However, there are other significant themes driving racial disparities. Future studies should focus on providing equitable access to care while also addressing these additional sources of disparities in HNC.
Shape memory polylactic acid/modified Eucommia ulmoides gum thermoplastic vulcanizates based on excellent interfacial adhesion and co‐continuous structure
Authors: Wang Yan , Pei Xianqiang , Pei Qianyao , Zhang Zhancheng , Zhang Yaoming , Wang Qihua , Wang Tingmei 
Published: January 2024

AbstractTowards the goal of developing renewable, biocompatible and intelligent polymer materials, natural Eucommia ulmoides gum (EUG) was modified via epoxidation and then the epoxidized EUG (EEUG) was compounded with another sustainable and biobased polymer, polylactic acid (PLA), to develop shape memory thermoplastic vulcanizates (TPVs) through reactive blending. The prepared PLA/EEUG TPVs displayed not only enhanced toughness but also greatly improved shape recovery ability, which was generated from the co‐continuous phase structure and excellent interfacial adhesion induced by in situ compatibilization during reactive blending. It is shown that dicumyl peroxide content exerted little influence on the toughness of the TPVs. However, heat‐triggered shape memory effects of the TPVs were significantly affected by the dicumyl peroxide content with a decrease in deformation ratio (Δε) from 163.51% to 128.36% and an increase in shape recovery ratio (Rr) from 73.32% to 91.93%. The findings of the present study offer an idea for the industrialization of biocompatible and smart materials for biomedical applications. © 2024 Society of Industrial Chemistry.
Energy, water, and protein folding: A molecular dynamics‐based quantitative inventory of molecular interactions and forces that make proteins stable
Authors: Galano‐Frutos Juan José , Sancho Javier 
Published: January 2024

AbstractProtein folding energetics can be determined experimentally on a case‐by‐case basis but it is not understood in sufficient detail to provide deep control in protein design. The fundamentals of protein stability have been outlined by calorimetry, protein engineering, and biophysical modeling, but these approaches still face great difficulty in elucidating the specific contributions of the intervening molecules and physical interactions. Recently, we have shown that the enthalpy and heat capacity changes associated to the protein folding reaction can be calculated within experimental error using molecular dynamics simulations of native protein structures and their corresponding unfolded ensembles. Analyzing in depth molecular dynamics simulations of four model proteins (CI2, barnase, SNase, and apoflavodoxin), we dissect here the energy contributions to ΔH (a key component of protein stability) made by the molecular players (polypeptide and solvent molecules) and physical interactions (electrostatic, van der Waals, and bonded) involved. Although the proteins analyzed differ in length, isoelectric point and fold class, their folding energetics is governed by the same quantitative pattern. Relative to the unfolded ensemble, the native conformations are enthalpically stabilized by comparable contributions from protein–protein and solvent–solvent interactions, and almost equally destabilized by interactions between protein and solvent molecules. The native protein surface seems to interact better with water than the unfolded one, but this is outweighed by the unfolded surface being larger. From the perspective of physical interactions, the native conformations are stabilized by van de Waals and Coulomb interactions and destabilized by conformational strain arising from bonded interactions. Also common to the four proteins, the sign of the heat capacity change is set by interactions between protein and solvent molecules or, from the alternative perspective, by Coulomb interactions.
Modeling analysis and experiment for energy system of ocean energy driven unmanned marine vehicle: The Yulang II case study
Authors: Jia Qi , Liao Yulei , Xu Peihong , Pang Shuo , Wang Bo , Li Ye 
Published: January 2024

AbstractIt is critical to master a marine robot's energy system characteristics adequately to ensure that the robot can perform long‐endurance tasks in complex and ever‐changing marine environments. This paper presents, for the first time, an energy system modeling and analysis method for a marine robot driven by wind, solar, and wave composite energy, using Yulang II as an example. First, an onshore experiment method was designed, and then the conversion experiment of wind, sunlight, and electrical energy was carried out based on that experiment method. At the same time, based on computational fluid dynamics technology, a resistance pole curve considering wave energy capture is constructed. In addition, the attitude, relative motion, and battery constraints of the robot in the ocean are analyzed to construct the energy system model of Yulang II. Finally, the method and model proposed in this paper were compared and verified by simulation and field tests. The results indicated that the corrected forecast deviation was 5.71% in the typical marine environment of the experiment, which supported the study of ocean energy‐driven unmanned marine vehicles strongly.