bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isohydric stomatal behaviour alters fruit vascular flows and minimizes fruit size reductions in drought-stressed ‘Hass’ avocado (Persea americana Mill.)
Authors: Kaneko Teruko , Gould Nick , Campbell David , Clearwater Michael J 
Published: February 2024

Abstract

Background and Aims
Plant water status is important for fruit development, because many fleshy fruits contain large amounts of water. However, there is no information on vascular flows of Persea americana ‘Hass’ avocado. The aims of this research were to explore the impact of drought stress on the water relationships of the ‘Hass’ avocado plant and its fruit growth.

Methods
Well-watered and water-stressed ‘Hass’ avocado plants were compared. Over 4 weeks, water flows through the shoot and fruit pedicel were monitored using external sap flow gauges. Fruit diameter was monitored using linear transducers, and stomatal conductance (gs), photosynthesis (A) and leaf and stem water potentials (Ѱleaf and Ѱstem) were measured to assess the response of the plants to water supply.

Key Results
In well-watered conditions, the average water inflow to the shoot was 72 g day−1. Fruit water inflow was 2.72 g day−1, but there was water loss of 0.37 g day−1 caused by the outflow (loss back into the tree) through the vascular tissues and 1.06 g day−1 from the fruit skin. Overall, fruit volume increased by 1.4 cm3 day−1. In contrast, water flow into fruit of water-stressed plants decreased to 1.88 g day−1, with the outflow increasing to 0.61 g day−1. As a result, increases in fruit volume were reduced to 0.4 cm3 day−1. The values of A, gs and sap flow to shoots were also reduced during drought conditions. Changes in the hourly time-courses of pedicel sap flow, fruit volume and stem water potential during drought suggest that the stomatal response prevented larger increases in outflow from the fruit. Following re-watering, a substantial recovery in growth rate was observed.

Conclusions
In summary, a reduction in growth of avocado fruit was observed with induced water deficit, but the isohydric stomatal behaviour of the leaves helped to minimize negative changes in water balance. Also, there was substantial recovery after re-watering, hence the short-term water stress did not decrease avocado fruit size. Negative impacts might appear if the drought treatment were prolonged.
Post-Digital Connectivities: Framing Offline Encounters in a Digital Prospection Space
Authors: Lyons Agnieszka , Tagg Caroline 
Published: February 2024

Abstract
This paper explores the use of mobile technologies in facilitating offline encounters, through a post-digital lens which posits the digital not as new or disruptive but as a ubiquitous and accepted part of everyday social connectivities. In the paper, we explore ways in which migrants draw on jointly assembled semiotic repertoires, affordances, and constraints of the digital space, as well as cultural knowledge and spatial relating, to establish common ground and an interpretative framework for engaging in ensuing offline encounters. Drawing on an interactional analysis of data from a large linguistic ethnographic project, we focus on how a group of Polish immigrants who live in different parts of London bring their offline contexts and socially or culturally motivated expectations into their interactions to facilitate alignment in interactional frames in the context of limited familiarity with each other. Overall, our analysis points to the role of group messaging in creating a digital prospection space in which a joint frame of reference can be interactively constructed in anticipation of an offline encounter.
The collision between human rights and arbitration: the game of inconsistencies at the Court of Arbitration for Sport
Authors: Shahlaei Faraz 
Published: February 2024

Abstract
This article depicts a clash between arbitration and international human rights law (IHRL) norms. It discusses the IHRL aspects of cases arbitrated by the Court of Arbitration for Sport (CAS). Procedural and substantive IHRL claims are being increasingly raised before CAS. However, CAS panels seem to be inconsistent in dealing with the issue. This article analyses the jurisprudence of CAS to identify inconsistencies, chaos, and contradictions in its approach to human rights claims. It also offers several factors that may contribute to this contradictory treatment of IHRL claims by CAS panels including the flexible nature of arbitral proceedings, inadequate expertise in IHRL among counsel and arbitrators, and the possibility of favouritism towards sport governing bodies. It concludes that the inconsistencies and contradictions in CAS jurisprudence make it difficult to regard it as a truly effective remedy for violations of human rights and that reforms are needed if human rights claims are to be given due consideration and the mechanism can be viewed as an effective remedy.
Localization of the Submandibular Glands Using High-Frequency Ultrasound
Authors: Jiang Liya , Hu Jintian , Jia Ying , Zhou Hongru , Su Xueshang , Zhuang Jun , Yuan Li , Zheng Qiaoyuan , Chai Hongli 
Published: February 2024

Abstract

Background
Injecting botulinum toxin (BTX) into the submandibular glands (SMGs) can treat drooling symptoms in neurological diseases and improve the aesthetics of SMG hypertrophy and ptotic SMGs.

Objectives
This study aimed to define the size and position of the SMGs by high-frequency ultrasound, and to perform statistical analysis to improve the safety and accuracy of BTX injection therapy.

Methods
Neck ultrasonography with high-frequency ultrasound was performed on 214 volunteers. The length, height, and thickness of the SMGs, and the distance between the SMGs and the midline, the anterior border of the sternocleidomastoid, the mandible, and the surface were measured.

Results
The SMGs were almond-shaped with a mean [standard deviation] length of 33.7 [4.7] mm, a thickness of 13.3 [2.9] mm, and a height of 27.6 [6.0] mm. The length and height were significantly different between underage and youth groups. The size of the SMGs did not show any notable differences with increasing BMI; however, their depth, and the distance from the mandible, midline, and anterior border of the sternocleidomastoid increased. No significant differences were observed between the affected and healthy sides in patients with microtia, hemifacial microsomia, or cleft lip and palate.

Conclusions
Ultrasound provides more comprehensive information regarding the size and position of the SMGs, which can serve as a reference in BTX therapy and in the diagnosis of SMG diseases involving size alterations.

Level of Evidence: 4
Molecular basis and functional development of membrane-based microbial metabolism
Authors: Yamada Mamoru 
Published: February 2024

ABSTRACT
My research interest has so far been focused on metabolisms related to the “membrane” of microorganisms, such as the respiratory chain, membrane proteins, sugar uptake, membrane stress and cell lysis, and fermentation. These basic metabolisms are important for the growth and survival of cell, and their knowledge can be used for efficient production of useful materials. Notable achievements in research on metabolisms are elucidation of the structure and function of membrane-bound glucose dehydrogenase as a primary enzyme in the respiratory chain, elucidation of ingenious expression regulation of several operons or by divergent promoters, elucidation of stress-induced programed-cell lysis and its requirement for survival during a long-term stationary phase, elucidation of molecular mechanism of survival at a critical high temperature, elucidation of thermal adaptation and its limit, isolation of thermotolerant fermenting yeast strains, and development of high-temperature fermentation and green energy production technologies. These achievements are described together in this review.
A comprehensive review of deep learning-based variant calling methods
Authors: Junjun Ren , Zhengqian Zhang , Ying Wu , Jialiang Wang , Yongzhuang Liu 
Published: February 2024

Abstract
Genome sequencing data have become increasingly important in the field of personalized medicine and diagnosis. However, accurately detecting genomic variations remains a challenging task. Traditional variation detection methods rely on manual inspection or predefined rules, which can be time-consuming and prone to errors. Consequently, deep learning–based approaches for variation detection have gained attention due to their ability to automatically learn genomic features that distinguish between variants. In our review, we discuss the recent advancements in deep learning–based algorithms for detecting small variations and structural variations in genomic data, as well as their advantages and limitations.
SpatialDDLS: an R package to deconvolute spatial transcriptomics data using neural networks
Authors: Mañanes Diego , Rivero-García Inés , Relaño Carlos , Torres Miguel , Sancho David , Jimenez-Carretero Daniel , Torroja Carlos , Sánchez-Cabo Fátima 
Published: February 2024

Abstract

Summary
Spatial transcriptomics has changed our way to study tissue structure and cellular organization. However, there are still limitations in its resolution, and most available platforms do not reach a single cell resolution. To address this issue, we introduce SpatialDDLS, a fast neural network-based algorithm for cell type deconvolution of spatial transcriptomics data. SpatialDDLS leverages single-cell RNA sequencing data to simulate mixed transcriptional profiles with predefined cellular composition, which are subsequently used to train a fully connected neural network to uncover cell type diversity within each spot. By comparing it with two state-of-the-art spatial deconvolution methods, we demonstrate that SpatialDDLS is an accurate and fast alternative to the available state-of-the art tools.

Availability and implementation
The R package SpatialDDLS is available via CRAN-The Comprehensive R Archive Network: https://CRAN.R-project.org/package=SpatialDDLS. A detailed manual of the main functionalities implemented in the package can be found at https://diegommcc.github.io/SpatialDDLS.
Tick-tock, racing the clock: parasitism is associated with decreased sprint performance in the Eastern fence lizard
Authors: Wild Kristoffer H , Gienger C M 
Published: February 2024

Abstract
Host–parasite relationships are important components of ecological systems that influence the evolution of both hosts and parasites. High levels of ectoparasitic infections can disrupt host homeostasis, causing adverse effects on health and performance. However, the effects of natural ectoparasite levels on host physiology are less understood, with most research designs implementing experimental or hormonal manipulations of hosts. In this study, we examined the interplay between tick parasitism and host characteristics on body condition and locomotor performance in Eastern fence lizards in natural settings. We found a higher frequency of tick infections in male lizards relative to females, with larger males being more likely to experience tick infection. Infected lizards had reduced locomotor performance, max sprint speed and 2-m run speed. Together this suggests an energetic trade-off between increased immune function and reduced locomotor performance, which is consistent with the immunocompetence–handicap hypothesis. Higher numbers of ticks on adult male lizards may be explained by age as well as the immunosuppressive effects of testosterone. Tick infection did not appear to reduce overall body condition of lizard hosts. Our findings shed light on the interplay between ectoparasitic infection, host characteristics, and locomotor performance under natural conditions. Such insights are crucial for understanding host–parasite dynamics and determining the trade-offs for hosts within ecological contexts.
Individualized treatment rule characterization via a value function surrogate
Authors: Freeman Nikki L B , Browder Sydney E , McGinigle Katharine L , Kosorok Michael R 
Published: February 2024

Abstract
Precision medicine is a promising framework for generating evidence to improve health and health care. Yet, a gap persists between the ever-growing number of statistical precision medicine strategies for evidence generation and implementation in real-world clinical settings, and the strategies for closing this gap will likely be context-dependent. In this paper, we consider the specific context of partial compliance to wound management among patients with peripheral artery disease. Using a Gaussian process surrogate for the value function, we show the feasibility of using Bayesian optimization to learn optimal individualized treatment rules. Further, we expand beyond the common precision medicine task of learning an optimal individualized treatment rule to the characterization of classes of individualized treatment rules and show how those findings can be translated into clinical contexts.
Clustering blood donors via mixtures of product partition models with covariates
Authors: Argiento Raffaele , Corradin Riccardo , Guglielmi Alessandra , Lanzarone Ettore 
Published: February 2024

ABSTRACT
Motivated by the problem of accurately predicting gap times between successive blood donations, we present here a general class of Bayesian nonparametric models for clustering. These models allow for the prediction of new recurrences, accommodating covariate information that describes the personal characteristics of the sample individuals. We introduce a prior for the random partition of the sample individuals, which encourages two individuals to be co-clustered if they have similar covariate values. Our prior generalizes product partition models with covariates (PPMx) models in the literature, which are defined in terms of cohesion and similarity functions. We assume cohesion functions that yield mixtures of PPMx models, while our similarity functions represent the denseness of a cluster. We show that including covariate information in the prior specification improves the posterior predictive performance and helps interpret the estimated clusters in terms of covariates in the blood donation application.
Nonparametric predictive model for sparse and irregular longitudinal data
Authors: Wang Shixuan , Kim Seonjin , Ryan Cho Hyunkeun , Chang Won 
Published: February 2024

Abstract
We propose a kernel-based estimator to predict the mean response trajectory for sparse and irregularly measured longitudinal data. The kernel estimator is constructed by imposing weights based on the subject-wise similarity on L2 metric space between predictor trajectories, where we assume that an analogous fashion in predictor trajectories over time would result in a similar trend in the response trajectory among subjects. In order to deal with the curse of dimensionality caused by the multiple predictors, we propose an appealing multiplicative model with multivariate Gaussian kernels. This model is capable of achieving dimension reduction as well as selecting functional covariates with predictive significance. The asymptotic properties of the proposed nonparametric estimator are investigated under mild regularity conditions. We illustrate the robustness and flexibility of our proposed method via extensive simulation studies and an application to the Framingham Heart Study.
Intravenous gentamicin therapy induces functional type VII collagen in patients with recessive dystrophic epidermolysis bullosa: an open-label clinical trial
Authors: Woodley David T , Hao Michelle , Kwong Andrew , Levian Brandon , Cogan Jon , Hou Yingping , Mosallaei Daniel , Kleinman Elana , Zheng Kate , Chung Claire , Kim Gene , Peng David , Chen Mei 
Published: February 2024

Abstract

Background
Recessive dystrophic epidermolysis bullosa (RDEB) is an incurable widespread blistering skin disorder caused by mutations in the gene encoding for type VII collagen (C7), the major component of anchoring fibrils.

Objectives
To evaluate the efficacy and safety of intravenous (IV) gentamicin readthrough therapy in patients with RDEB harbouring nonsense mutations. The primary outcomes were increased expression of C7 in patients’ skin and safety assessments (ototoxicity, nephrotoxicity, autoimmune response); secondary outcomes included measuring wound healing in target wounds and assessment by a validated Epidermolysis Bullosa Disease Activity and Scarring Index (EBDASI) scoring system.

Methods
An open-label pilot trial to assess two different IV gentamicin regimens between August 2018 and March 2020 with follow-up through to 180 days post-treatment was carried out. Three patients with RDEB with confirmed nonsense mutations in COL7A1 in either one or two alleles and decreased baseline expression of C7 at the dermal–epidermal junction (DEJ) of their skin participated in the study. Three patients received gentamicin 7.5 mg kg–1 daily for 14 days and two of the three patients further received 7.5 mg kg–1 IV gentamicin twice weekly for 12 weeks. Patients who had pre-existing auditory or renal impairment, were currently using ototoxic or nephrotoxic medications, or had allergies to aminoglycosides or sulfate compounds were excluded.

Results
After gentamicin treatment, skin biopsies from all three patients (age range 18–28 years) exhibited increased C7 in their DEJ. With both regimens, the new C7 persisted for at least 6 months post-treatment. At 1 and 3 months post-treatment, 100% of the monitored wounds exhibited &gt; 85% closure. Both IV gentamicin infusion regimens decreased EBDASI total activity scores. Of the patients assessed with the EBDASI, all exhibited decreased total activity scores 3 months post-treatment. All three patients completed the study; no adverse effects or anti-C7 antibodies were detected.

Conclusions
IV gentamicin induced the readthrough of nonsense mutations in patients with RDEB and restored functional C7 in their skin, enhanced wound healing and improved clinical parameters. IV gentamicin may be a safe, efficacious, low-cost and readily available treatment for this population of patients with RDEB.
Can diffusion tensor imaging unlock the secrets of the growth plate?
Authors: Kvist Ola , Santos Laura A , De Luca Francesca , Jaramillo Diego 
Published: February 2024

Abstract
“How tall will I be?” Every paediatrician has been asked this during their career. The growth plate is the main site of longitudinal growth of the long bones. The chondrocytes in the growth plate have a columnar pattern detectable by diffusion tensor imaging (DTI). DTI shows the diffusion of water in a tissue and whether it is iso- or anisotropic. By detecting direction and magnitude of diffusion, DTI gives information about the microstructure of the tissue. DTI metrics include tract volume, length, and number, fractional anisotropy (FA), and mean diffusivity. DTI metrics, particularly tract volume, provide quantitative data regarding skeletal growth and, in conjunction with the fractional anisotropy, be used to determine whether a growth plate is normal. Tractography is a visual display of the diffusion, depicting its direction and amplitude. Tractography gives a more qualitative visualization of cellular orientation in a tissue and reflects the activity in the growth plate. These two components of DTI can be used to assess the growth plate without ionizing radiation or pain. Further refinements in DTI will improve prediction of post-imaging growth and growth plate closure, and assessment of the positive and negative effect of treatments like cis-retinoic acid and growth hormone administration.
Unravelling the evolutionary history and promoting conservation genetics of Anadenanthera colubrina var. cebil (Leguminosae), a paradigmatic species in Seasonally Dry Tropical Forests
Authors: Zerda Moreira Andrea , García María Victoria , Barrandeguy María Eugenia 
Published: February 2024

Abstract
Seasonally Dry Tropical Forests (SDTFs) exhibit a patchy distribution throughout the Neotropics and are considered one of the most threatened tropical forests in the world. Nonetheless, their conservation has often been neglected in comparison to efforts directed towards other biomes. The distribution pattern of Anadenanthera colubrina (Vell.) Brenan var. cebil (Griseb) Altschul allows an adequate mapping of this biome. The main objectives of this work are to study the historical processes that determined the current distribution of A. colubrina var. cebil under the hypothesis that the demographic history of the remaining fragments of SDTFs in Argentina leads back to a single event of ancestral divergence, and identify Argentinean localities with priority for conservation. Plastid genetic diversity of A. colubrina var. cebil from northern Argentina is characterised using three plastid microsatellite loci. From this, genetic characterization, population genetics, and Bayesian inference were carried out. The results from approximate Bayesian computations show that the Misiones and Piedmont Subandean nuclei diverged from an ancestral population in the early Neogene while the Formosa population derived from the Misiones nucleus in the late Neogene. Two out of five locations from the Misiones nucleus and three of nine locations from the Subandean Piedmont nucleus are proposed as priority conservation areas.
Trends of fruit morphogenesis in Pandanaceae: comparative carpology of Freycinetia Gaudich
Authors: Bobrov Alexey V F Ch , Zdravchev Nikita S , Romanov Mikhail S , Iovlev Petr S , Mikhaylova Anna A , Kuptsov Kirill V , Timchenko Anton S , Vasekha Natalia D , Sorokin Alexey N 
Published: February 2024

Abstract
With the current reassessment of the phylogeny of Pandanales, revealing the peculiarities of fruit structure of representatives of the order, as well as determination of apomorphies and plesiomorphies of the families included in the order are of importance. With the aim to fill in gaps in the data on fruit structure and to determine principal trends of fruit morphogenesis in Pandanaceae, the present study focused on pericarp anatomy in the genus Freycinetia, fruits of which are traditionally referred to as berries. The present investigation confirmed the lack of a continuous sclerenchymatous zone in the pericarp of Freycinetia species, and the fruit is treated as a superior paracarpous berry of Nuphar type. Fusiform groups of longitudinally elongated sclereids are revealed in the mesocarp of Freycinetia palawanensis (incertae sedis) and F. graminifolia (section Solmsiella). Since this character is treated as specific for the subgenus Gaudichaudiella, the inclusion of these two studied species in this subgenus is proposed. The principal carpological plesiomorphies and apomorphies of the family are outlined, and two scenarios for fruit morphogenesis, from berries to ‘drupaceous’ fruits and vice versa, are proposed.