bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENDAMPINGAN PRODUKSI MaMiN BERBAHAN BAKU DAUN KELOR PADA KELOMPOK KADER ODGJ
Authors: Rulianah Sri , Prayitno Prayitno , Susilo Sugeng Hadi , Hidayatinnisa Nurul 
Published: January 2024

The ODJG group (People in Mental Disorders) is a community group engaged in social activities: helping, guidance, and mental health rehabilitation for ODJG who have undergone psychiatric medical treatment from a Mental Hospital. To carry out its activities, this group formed a business that produces food and beverages (MaMin) made from Moringa leaves (moringa tea, instant Moringa, Moringa ice wax, Moringa yogurt, Moringa pudding, Moringa flower extract). where the products have been marketed in Malang and surrounding areas. However, the production process encountered several obstacles: the lack of production equipment, the lack of maintaining product quality and the health of the work environment, and the lack of marketing techniques. Community service (PkM) aims to increase partners' ability to produce quality, safe, dan healthy MaMin made from Moringa leaves. The methods used in solving partner problems: 1). Production equipment grants, 2). Excellent and safe production technique training (GMP and HACCP),
3). Marketing digital training (digital marketing), 4). Production and marketing assistance and consulting. The results of the PkM activity show that there is an increase in the knowledge and skills of partners in producing healthy and quality MaMin and marketing it with digital marketing media
Association of pancreatitis with risk of diabetes: analysis of real-world data
Authors: Ba Djibril M. , Chinchilli Vernon M. , Cozzi Anna M. , Bradley David P. , Pichardo-Lowden Ariana R. 
Published: January 2024

IntroductionDiabetes is a major cause of disease burden with considerable public health significance. While the pancreas plays a significant role in glucose homeostasis, the association between pancreatitis and new onset diabetes is not well understood. The purpose of this study was to examine that association using large real-world data.Materials and methodsUtilizing the IBM® MarketScan® commercial claims database from 2016 to 2019, pancreatitis and diabetes regardless of diagnostic category, were identified using International Classification of Diseases, Tenth Revision [ICD-10] codes. We then performed descriptive analyses characterizing non-pancreatitis (NP), acute pancreatitis (AP), and chronic pancreatitis (CP) cohort subjects. Stratified Cox proportional hazards regression models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CI) of diabetes across the three clinical categories.ResultsIn total, 310,962 individuals were included in the analysis. During 503,274 person‐years of follow‐up, we identified 15,951 incident diabetes cases. While men and women had higher incidence rates of CP and AP-related diabetes, the rates were significantly greater in men and highest among individuals with CP (91.6 per 1000 persons-years (PY)) followed by AP (75.9 per 1000-PY) as compared to those with NP (27.8 per 1000-PY). After adjustment for diabetes risk factors, relative to the NP group, the HR for future diabetes was 2.59 (95% CI: 2.45-2.74) (P&lt;0.001) for the CP group, and 2.39 (95% CI: 2.30-2.48) (P&lt;0.001) for the AP group.ConclusionPancreatitis was associated with a high risk of diabetes independent of demographic, lifestyle, and comorbid conditions.
Green extraction of puromycin-based antibiotics from Streptomyces albofaciens (MS38) for sustainable biopharmaceutical applications
Authors: Singh Neha , Patil Sandip , Shahnawaz Mohd. , Rai Vibhuti , Patil Abhinandan , Tripathi C. K. M. , Wen Feiqiu , Dong Shaowei , Cai Defeng 
Published: January 2024

Background: Microbial secondary metabolites have shown promise as a source of novel antimicrobial agents. In this study, we aimed to isolate, characterize, and evaluate the antimicrobial activity of compound from a novel Streptomyces albofaciens strain MS38. The objective was to identify a potential bioactive compound with broad-spectrum antimicrobial properties.Methods: The isolated strain MS38 on starch casein agar was characterized using morphological, physiological, and molecular identification techniques. The compound was obtained from the fermented broth through extraction with n-butanol and further purification using silica gel column chromatography and high-performance liquid chromatography (HPLC). Structural elucidation was conducted using Ultraviolet (UV), Infrared (IR), nuclear magnetic resonance (NMR), and mass spectrometry (MS) techniques. The antimicrobial activity was evaluated using the agar well diffusion method and the microplate Alamar blue assay (MABA).Results: The isolated strain MS38 was identified as novel S. albofaciens based on morphological characteristics and confirmed by 16S sequences analysis and MALDI-TOF MS. The compound obtained from the fermented broth exhibited substantial antimicrobial activity against a variety of pathogenic bacteria and fungi. Structural analysis revealed a complex chemical structure with characteristic functional groups indicative of potential antimicrobial properties. The compound demonstrated strong activity against both Gram-positive (Staphylococcus Spp.) and Gram-negative (Klebsiella pneumoniae and Escherichia coli) bacteria, as well as fungi, including Candida albicans and Trichophyton rubrum.Conclusion: This study successfully isolated and characterized a bioactive compound from a novel S. albofaciens MS38. The compound exhibited significant antimicrobial activity against a range of pathogenic microorganisms. These findings underscore the importance of exploring microbial biodiversity for the discovery of novel antimicrobial agents. This study contributes to the growing knowledge of microbial secondary metabolites with potential therapeutic value.
Eliminating the invading extracellular and intracellular FnBp+ bacteria from respiratory epithelial cells by autophagy mediated through FnBp-Fn-Integrin α5β1 axis
Authors: Meng Meiqi , Wang Jiachao , Li Hongru , Wang Jiao , Wang Xuan , Li Miao , Gao Xue , Li Wenjian , Ma Cuiqing , Wei Lin 
Published: January 2024

BackgroundWe previously found that the respiratory epithelial cells could eliminate the invaded group A streptococcus (GAS) through autophagy induced by binding a fibronectin (Fn) binding protein (FnBp) expressed on the surface of GAS to plasma protein Fn and its receptor integrin α5β1 of epithelial cells. Is autophagy initiated by FnBp+ bacteria via FnBp-Fn-Integrin α5β1 axis a common event in respiratory epithelial cells?MethodsWe chose Staphylococcus aureus (S. aureus/S. a) and Listeria monocytogenes (L. monocytogenes/L. m) as representatives of extracellular and intracellular FnBp+ bacteria, respectively. The FnBp of them was purified and the protein function was confirmed by western blot, viable bacteria count, confocal and pull-down. The key molecule downstream of the action axis was detected by IP, mass spectrometry and bio-informatics analysis.ResultsWe found that different FnBp from both S. aureus and L. monocytogenes could initiate autophagy through FnBp-Fn-integrin α5β1 axis and this could be considered a universal event, by which host tries to remove invading bacteria from epithelial cells. Importantly, we firstly reported that S100A8, as a key molecule downstream of integrin β1 chain, is highly expressed upon activation of integrin α5β1, which in turn up-regulates autophagy.ConclusionsVarious FnBp from FnBp+ bacteria have the ability to initiate autophagy via FnBp-Fn-Integrin α5β1 axis to promote the removal of invading bacteria from epithelial cells in the presence of fewer invaders. S100A8 is a key molecule downstream of Integrin α5β1 in this autophagy pathway.
TNIK regulation of interferon signaling and endothelial cell response to virus infection
Authors: Chau Khanh M. , Dominic Abishai , Davis Eleanor L. , Kotla Sivareddy , Berrios Estefani Turcios , Fahim Arsany , Arunesh Ashwin , Li Shengyu , Zhao Dongyu , Chen Kaifu , Davis Alan R. , Nguyen Minh T. H. , Wang Yongxing , Evans Scott E. , Wang Guangyu , Cooke John P. , Abe Jun-ichi , Huston David P. , Le Nhat-Tu 
Published: January 2024

BackgroundTraf2 and Nck-interacting kinase (TNIK) is known for its regulatory role in various processes within cancer cells. However, its role within endothelial cells (ECs) has remained relatively unexplored.MethodsLeveraging RNA-seq data and Ingenuity Pathway Analysis (IPA), we probed the potential impact of TNIK depletion on ECs.ResultsExamination of RNA-seq data uncovered more than 450 Differentially Expressed Genes (DEGs) in TNIK-depleted ECs, displaying a fold change exceeding 2 with a false discovery rate (FDR) below 0.05. IPA analysis unveiled that TNIK depletion leads to the inhibition of the interferon (IFN) pathway [-log (p-value) &gt;11], downregulation of IFN-related genes, and inhibition of Hypercytokinemia/Hyperchemokinemia [-log (p-value) &gt;8]. The validation process encompassed qRT-PCR to evaluate mRNA expression of crucial IFN-related genes, immunoblotting to gauge STAT1 and STAT2 protein levels, and ELISA for the quantification of IFN and cytokine secretion in siTNIK-depleted ECs. These assessments consistently revealed substantial reductions upon TNIK depletion. When transducing HUVECs with replication incompetent E1-E4 deleted adenovirus expressing green fluorescent protein (Ad-GFP), it was demonstrated that TNIK depletion did not affect the uptake of Ad-GFP. Nonetheless, TNIK depletion induced cytopathic effects (CPE) in ECs transduced with wild-type human adenovirus serotype 5 (Ad-WT).SummaryOur findings suggest that TNIK plays a crucial role in regulating the EC response to virus infections through modulation of the IFN pathway.
Overview of early cretaceous gold mineralization in the orogenic belt of the eastern margin of the Siberian craton: geological and genetic features
Authors: Goryachev Nikolay , Fridovsky Valery 
Published: January 2024

The giant Verkhoyansk-Kolyma gold province, producing more than∼ 3700 t of gold, is one of the most important metallogenic provinces for orogenic gold deposits in the world. The province is located on the eastern margin of the Siberian craton. The main types here are multistage orogenic Au-As and orogenic-like Au-Sb types of mineralization, related to the Late Jurassic-Early Cretaceous metallogeny and the evolution of the convergent margin. These deposits were formed in the environment of late orogeny during two metallogenic stages—the Late Jurassic-Early Cretaceous collision-related (early stage/stage one, 135–150 Ma) and the Early Cretaceous subduction-related (late stage/stage two 114–130 Ma). The common features, differences and genetic nature of the deposits of these two metallogenic stages have been debated for a long time. To improve the understanding of these problematic issues and the genesis of deposits, and control over them by the mantle lithosphere, we consider here the Early Cretaceous orogenic belts of the eastern margin of the Siberian craton. Orogenic belts are related to the development of the paleo-Arctic and paleo-Pacific margins of Siberia. The tectonic, geochronological, mineralogical and geochemical specificity of the deposits of the orogenic belts are shown. A regional metallogenic overview of gold deposits is performed using data on the age of mineralization and the isotopic composition of sulfur sulfides. A preliminary model of the origin of late stage mineralization is proposed in connection with the processes of the Okhotsk-Koryak orogenic belt formation. Increased concentrations of siderophilic and chalcophilic elements, the Ni, Bi minerals presence in the ores, relatively high fineness of gold, predominance of juvenile sulfur in the isotopic composition of sulfide sulfur, аnd the control by large trans-crustal faults as well as spatial and chronological association with initial (dike) magmatism indicate mantle sources of ore fluids. This mineralization within the orogenic belts of the eastern margin of the Siberian craton assumingly appeared due to dehydration of the submerged slab and local upwelling in the mantle in the rear of the active continental margin. The possibility of the gold-bearing fluids existence in such conditions is estimated. The metallogenic specific nature of collision-related and subduction-related orogenic Au is defined. The proposed model can be useful for the analysis of polychronous orogenic Au-As and orogenic-like Au-Sb metallogeny of orogenic belts on craton margins globally.
Rockburst hazard assessment and prevention and control strategies in the Ruihai gold mine
Authors: Li Pengxiang , Wang Zongwen , Chen Bingrui , Peng Jianping , Zhang Bochao , Zhao Jinshuai , Xie Houlin 
Published: January 2024

Ruihai gold mine is a super large underwater gold mine with an initial mining depth of more than 1,400 m. Deep mining may face rockburst disaster. In order to scientifically control the potential rockburst risk, ensure the safety of underground personnel and equipment, and realize the safe and efficient mining of the mine, it is necessary to evaluate the rockburst risk of the mine and formulate appropriate rockburst risk warning and mitigation strategies. XRD mineral composition analysis and conventional rock mechanical tests were carried out on the parent rock granite samples. The results show that there are differences in the lithologic composition and rock mechanics parameters of the three colors of granite, which are preliminarily determined as three kinds of granite; The rockburst tendency of three kinds of granites is evaluated by using three classical rockburst criteria, and the discrimination results of the three rockburst criteria are not completely consistent or even contradictory. The application limitations rockburst risk assessment based on index criteria are discussed and analyzed in detail. It is proposed to apply microseismic monitoring technology to warn and manage the rockburst risk of Ruihai gold mine in development and mining stages, and put forward microseismic monitoring layout schemes and targeted rockburst prevention and control measures in different stages. The research results can provide reference for similar projects.
Chemogenetic activation of PVN CRH neurons disrupts the estrous cycle and LH dynamics in female mice
Authors: Yu Junru , Li Xiao-Feng , Tsaneva-Atanasova Krasimira , Zavala Eder , O’Byrne Kevin T. 
Published: January 2024

IntroductionThe impact of stress on reproductive function is significant. Hypothalamic paraventricular nucleus (PVN) corticotrophin-releasing hormone (CRH) plays a major role in regulating the stress response. Understanding how the hypothalamic-pituitary-adrenal (HPA) axis and the hypothalamic-pituitary-gonadal (HPG) axis interact is crucial for comprehending how stress can lead to reproductive dysfunction. However, whether stress influences reproductive function via modulating PVN CRH or HPA sequelae is not fully elucidated.MethodsIn this study, we investigated the impact of chemogenetic activation of PVN CRH neurons on reproductive function. We chronically and selectively stimulated PVN CRH neurons in female CRH-Cre mice using excitatory designer receptor exclusively activated by designer drugs (DREADDs) viral constructs, which were bilaterally injected into the PVN. The agonist compound-21 (C21) was delivered through the drinking water. We determined the effects of DREADDs activation of PVN CRH neurons on the estrous cycles, LH pulse frequency in diestrus and metestrus and LH surge in proestrus mice. The effect of long-term C21 administration on basal corticosterone secretion and the response to acute restraint stress during metestrus was also examined. Additionally, computer simulations of a mathematical model were used to determine the effects of DREADDs activation of PVN CRH neurons, simulating chronic stress, on the physiological parameters examined experimentally.ResultsAs a result, and consistent with our mathematical model predictions, the length of the estrous cycle was extended, with an increase in the time spent in estrus and metestrus, and a decrease in proestrus and diestrus. Additionally, the frequency of LH pulses during metestrus was decreased, but unaffected during diestrus. The occurrence of the preovulatory LH surge during proestrus was disrupted. The basal level of corticosterone during metestrus was not affected, but the response to acute restraint stress was diminished after long-term C21 application.DiscussionThese data suggest that PVN CRH neurons play a functional role in disrupting ovarian cyclicity and the preovulatory LH surge, and that the activity of the GnRH pulse generator remains relatively robust during diestrus but not during metestrus under chronic stress exposure in accordance with our mathematical model predictions.
Industrial agglomeration and PM2.5 pollution in Yangtze River Economic Belt in China: non-linear estimation and mechanism analysis
Authors: Li Lin , Xia Zhexi , Yi Jiahui , Qi Rui , Cheng Jinhua 
Published: January 2024

Introduction: The uncertainty associated with PM2.5 pollution hinders the economic high-quality development and threatens public health. Industrial agglomeration stands as a critical factor in regional economic and environmental governance, and the current studies about its impact on PM2.5 pollution are mostly limited to a specific industry or unidirectional influence.Methods: Our study constructed spatial econometric models to analyze the effect of three major industrial agglomerations on PM2.5 pollution, based on evidence from 110 prefecture-level cities of the Yangtze River Economic Belt in 2005–2019.Results: The results show that: 1) The three major industrial agglomerations and PM2.5 pollution present different spatiotemporal characteristics and show prominent positive spatial autocorrelation and agglomeration effect. 2) The primary industrial agglomeration contributes to a decrease in PM2.5 pollution and exhibits negative spatial spillover effects. A nonlinear relationship is observed between the secondary industrial agglomeration and PM2.5 pollution. The tertiary industrial agglomeration results in an increase in PM2.5 pollution. 3) The effects of secondary industrial agglomeration on PM2.5 pollution exhibit varying degrees of ‘inverted U-shape’ curves in the upstream, midstream, and downstream cities. The midstream cities are the first to reach the inflection point of agglomeration. 4) Industrial agglomeration affects PM2.5 pollution through three mechanisms, including scale expansion effect, technological spillover effect, and population scale effect.Discussion: Based on the empirical findings, this study provides scientific support and decision-making reference to improve the positive impacts of industrial agglomerations on PM2.5 pollution.
Multi-dimensional temperature sensitivity of protected tropical mountain rain forests
Authors: Finegan Bryan , Delgado Diego , Hernández Gordillo Alba Lorena , Zamora Villalobos Nelson , Núñez Florez Rafael , Díaz Santos Fabio , Vílchez Mendoza Sergio 
Published: January 2024

IntroductionTropical mountain rain forests (TMRF, natural forests at &gt; 300 m asl) are globally important for biodiversity and ecosystem services and are believed to be highly vulnerable to climate change. But there are no specific approaches for rigorous assessment of their vulnerability at the landscape and local scales necessary for management for adaptation. We address the challenge of evaluating the ecological sensitivity to temperature of TMRF, applying a multidimensional approach in protected areas over a 440–2,950 m asl altitudinal gradient in Costa Rica, synthesizing results of a long-term research programme (2012-present). We evaluate the sensitivity to the current spatial temperature gradient of eleven ecosystem properties in three categories: forest composition and diversity, thermal characteristics of forest stands and forest structure and dynamics.MethodsData are from 29 to 32 plots of 50 m x 50 m (0.25 ha) distributed over the gradient, in which all trees, palms and tree ferns ≥ 10 dbh are identified to species and measured for recruitment, growth and mortality. An experimental study of leaf litter decomposition rates was carried out in twelve plots. Current and future (SSP 585, 2070) values of mean annual temperatures MAT were obtained from online climate surfaces. Thermal characteristics of forest stands were determined using MATs of species occurrences in GBIF and include a new index, the Community Thermal Capital Index (CTCI), calculated as CTI-MAT.ResultsWe classified degrees of sensitivity to temperature as very weak, weak, moderate or substantial. All eleven ecosystem properties are substantially sensitive, so changes in their values are expected under rising temperatures. Species density, the community temperature index CTI, tree recruitment and mortality rates and leaf litter decomposition rates are positively related to temperature, while the community weighted mean thermal niche breadth, the CTCI, net basal area increments, stand basal area and carbon in aboveground biomass are negatively related. Results point to zones of vulnerability in the protected areas.DiscussionIn montane forests, positive values of the CTCI–climate credit– robust basal area growth and very low mortality and leaf litter decomposition rates suggest healthy ecosystems and no risk of mountaintop extinction. Lowland forests may be vulnerable to degradation and biotic attrition, showing current basal area loss, high mortality and climate debts. National and local actors are participating in a process of adoption of the sensitivity analysis and recommendations regarding zones of vulnerability.
Virus infection pattern imprinted and diversified the differentiation of T-cell memory in transcription and function
Authors: Wang Yuan , Mei Xinyue , Lin Zhengfang , Yang Xiaoyun , Cao Jinpeng , Zhong Jiaying , Wang Junxiang , Cheng Li , Wang Zhongfang 
Published: January 2024

IntroductionMemory T (Tm) cells are a subpopulation of immune cells with great heterogeneity. Part of this diversity came from T cells that were primed with different viruses. Understanding the differences among different viral-specific Tms will help develop new therapeutic strategies for viral infections.MethodsIn this study, we compared the transcriptome of Tm cells that primed with CMV, EBV and SARS-CoV-2 with single-cell sequencing and studied the similarities and differences in terms of subpopulation composition, activation, metabolism and transcriptional regulation.ResultsWe found that CMV is marked by plentiful cytotoxic Temra cells, while EBV is more abundant in functional Tem cells. More importantly, we found that CD28 and CTLA4 can be used as continuous indicators to interrogate the antiviral ability of T cells. Furthermore, we proposed that REL is a main regulatory factor for CMV-specific T cells producing cytokines and plays an antiviral role.DiscussionOur data gives deep insight into molecular characteristics of Tm subsets from different viral infection, which is important to understand T cell immunization. Furthermore, our results provide basic background knowledges for T cell based vaccine development in future.