bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00629, a HOXB4‐downregulated long noncoding RNA, inhibits glycolysis and ovarian cancer progression by destabilizing c‐Myc
Authors: Liu Jia , Zhu Yuan , Wang Huan , Han Chuanchun , Wang Yongpeng , Tang Ranran 
Published: January 2024

AbstractOvarian cancer (OC) cells typically reprogram their metabolism to promote rapid proliferation. However, the role of long noncoding RNAs (lncRNAs) in the metabolic reprogramming of ovarian cancer, especially in glucose metabolic reprogramming, remains largely unknown. LINC00629 has been reported in our previous study to promote osteosarcoma progression. Upregulated LINC00629 was found to enhance the growth‐suppressive effect of apigenin on oral squamous cell carcinoma. However, the precise function of LINC00629 in ovarian cancer development remains poorly understood. In this study, we found that LINC00629 was significantly downregulated in OC tissues and that low LINC00629 expression was associated with poor survival. Inhibition of LINC00629 was required for increased glycolysis activity and cell proliferation in ovarian cancer. In vivo, overexpression of LINC00629 dramatically inhibited tumor growth and lung metastasis. Mechanistically, LINC00629 interacted with and destabilized c‐Myc, leading to its ubiquitination and proteasome degradation, further resulting in increased expression of downstream glycolysis‐related genes and glucose metabolic reprogramming in OC. Interestingly, HOXB4 bound to the LINC00629 promoter and inhibited its transcription, indicating that LINC00629 is a transcriptional target of HOXB4. Collectively, these findings establish a direct role for LINC00629 in suppressing glucose metabolism, and HOXB4/LINC00629/c‐Myc might serve as a potential biomarker and an effective therapeutic strategy for OC cancer treatment.
Achaete‐scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression
Authors: Kato Yutaka , Fukazawa Takahiro , Tanimoto Keiji , Kanawa Masami , Kojima Masato , Saeki Isamu , Kurihara Sho , Touge Ryo , Hirohashi Nobuyuki , Okada Satoshi , Hiyama Eiso 
Published: January 2024

AbstractAchaete‐scute family bHLH transcription factor 2 (ASCL2) is highly expressed in hepatoblastoma (HB) tissues, but its role remains unclear. Thus, biological changes in the HB cell line HepG2 in response to induced ASCL2 expression were assessed. ASCL2 expression was induced in HepG2 cells using the Tet‐On 3G system, which includes doxycycline. Cell viability, proliferation activity, mobility, and stemness were evaluated using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, colony‐formation, migration, invasion, and sphere‐formation assays. Quantitative reverse‐transcription polymerase chain reaction was used to assess the expression of markers for proliferation (CCND1 and MYC), epithelial‐mesenchymal transition (EMT; SNAI1, TWIST1, and ZEB1), mesenchymal‐epithelial transition (CDH1), and stemness (KLF4, POU5F1, and SOX9). Compared with the non‐induced HepG2 cells, cells with induced ASCL2 expression showed significant increases in viability, colony number, migration area (%), and sphere number on days 7, 14, 8, and 7, respectively, and invasion area (%) after 90 h. Furthermore, induction of ASCL2 expression significantly upregulated CCND1, MYC, POU5F1, SOX9, and KLF4 expression on days 2, 2, 3, 3, and 5, respectively, and increased the ratios of SNAI1, TWIST1, and ZEB1 to CDH1 on day 5. ASCL2 promoted the formation of malignant phenotypes in HepG2 cells, which may be correlated with the upregulation of the Wnt signaling pathway‐, EMT‐, and stemness‐related genes. ASCL2 activation may therefore be involved in the progression of HB.
Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination
Authors: Yang Min , Li Ling 
Published: January 2024

AbstractBronchopneumonia is the most common pneumonia in childhood. Therefore, we tested the effects of Remimazolam presented Bronchopneumonia and its possible mechanisms. Phillygenin increased survival rate, reduced W/D ratio, and lung injury score, and inhibited IL‐1β, IL‐6, TNF‐α, and INF‐γ levels in mice model of bronchopneumonia. Remimazolam induced PDPK1 and p‐AKT protein expressions, and suppressed NLRP3 protein expression in lung tissue of mice model. In vitro model, Remimazolam also induced PDPK1 and p‐AKT protein expressions, and suppressed NLRP3 protein expression. Remimazolam also inhibited inflammation levels in vitro model. PDPK1 inhibitor, PHT‐427 (100 mg/kg) reduced survival rate, increased W/D ratio and lung injury score, and promoted inflammation levels in mice model of bronchopneumonia by treated with Remimazolam. PHT‐427 suppressed PDPK1 and p‐AKT protein expressions and induced NLRP3 protein expression in mice model of bronchopneumonia by treated with Remimazolam. Remimazolam interlinked PDPK1 protein. Remimazolam increased the expressions of PDPK1 and p‐AKT in vitro model. Remimazolam reduced PDPK1 ubiquitination in vitro model.
An analysis of interagency collaboration: Lessons learned from Colorado's early intervention program
Authors: Ollerenshaw Lindsay V. , McManus Beth , Rapport Mary Jane , Perraillon Marcelo , Murphy Natalie J. , Magnusson Dawn 
Published: January 2024

AbstractIntroductionThe purpose of this study was to describe interagency collaboration in Part C Early Intervention (EI) programs.MethodsBetween 18 April and 9 May 2022, 48 EI service coordinators (SCs) from 14 programs in one state completed adapted versions of the Interagency Collaboration Activities Scale (IACAS) and Relational Coordination Survey (RCS). Assessing perceptions of shared structures (IACAS) and coordination quality (RCS), these combined measures summarized interagency collaboration with 11 organizations. Mean (SD) survey responses were estimated and ranked.ResultsSample SCs represent a breadth of professional disciples and vary substantially in their current and desired collaborations. The quantity of SCs collaborating with organizations ranged from 0% to 98%. Nearly all (98%) reported collaborating with the school districts, few (15%) reported collaborating with insurance, and none reported collaborating with Supplemental Nutrition Assistance Program or Special Supplemental Nutrition Program for Women, Infants, and Children. The majority of SCs expressed desire to increase their collaborations with most of the listed organizations. The perceived quality of collaborations varied substantially at both individual and organizational levels. When comparing SC perceptions of shared structures and coordination quality, SCs reported more favourable collaborations with school districts (IACAS rank: first and RCS rank: first) and less favourable collaborations with insurance (IACAS rank: ninth and RCS rank: seventh). Some organizations rankings varied across both scales, including pediatric primary care (IACAS rank: third and RCS rank: eighth) and hospitals (IACAS rank: sixth and RCS rank: second). Overall, SCs reported low perceived existence of shared structures while coordination quality varied by organization. Opportunities for collaborative growth were identified.DiscussionDespite its importance and required implementation in EI, perceptions of interagency collaboration varied substantially within and between EI programs. There is a suggested need to increase the quantity of SCs that collaborate and identified opportunities to increase the quality of collaborations that already exist.
Blended feeding in gastrostomy‐fed children—A scoping review
Authors: Doyle Carmel , Louw Julia , Shovlin Amanda , Dowd Laura , Kavanagh Maria 
Published: January 2024

AbstractBackgroundBlended feeding has become increasingly prevalent in recent years with its practice gaining some momentum. With anecdotal reports of benefits and little evidence of harm in the literature regarding blended feeding, this scoping review was deemed important. The aim of this scoping review was to summarise the published evidence about blended feeding in gastrostomy‐fed children.MethodThe scoping review methodology used included searches in specific online databases: PUBMED, PsychINFO, CINAHL, SCOPUS, AMED and EMBASE for articles that addressed issues pertaining to blended feeds in gastrostomy‐fed children. Grey literature was also considered. Inclusion criteria included papers that pertained to information and research on blended feeding in gastrostomy‐fed children. Studies published in English over the past 11 years (2011–2022) were included. This resulted in 59 papers being included in this scoping review.ResultsThematic analysis of the literature identified eight overall themes. It was clear that parents found blended feeding promoted the normalising of feeding, their own involvement in decision‐making around foods and promotion of a socially inclusive mealtime. The need for dietician and health professional input and support is key, whereas a lack of guidelines acts as a barrier to blended feeding. Furthermore, risks associated with blended feeding are identified but also the benefits to the physical well‐being of the child are considered.ConclusionThe review was comprehensive in that it identified a broad range of literature, exploring the extent, range and nature of research activity related to the use of blended feeds. The lack of original research is a concern. However, it is expected this review will provide direction for researchers, and in particular inform policy and practitioners working in the field where blended feeds may be an option for gastrostomy‐fed children.
Investigating the perceptions and experiences of Canadian dentists on dental regulatory bodies' communications and guidelines during the COVID‐19 pandemic
Authors: McLaughlin Kendra Jennie , Khanna Mehak , Allison Paul J. , Glogauer Michael , McNally Mary E. , Quiñonez Carlos , Rock Leigha , Siqueira Walter , Madathil Sreenath A. 
Published: January 2024

AbstractObjectiveDental regulatory bodies aim to ensure the health and safety of dentists, dental staff patients and the public. An important responsibility during a pandemic is to communicate risk and guidelines for patient care. Limited data exist on the perceptions and experiences of dentists navigating new guidelines for mitigating risk in dental care during the pandemic. The objective of this study was to use a qualitative approach to explore how dentists in Canada experienced and perceived their regulatory bodies' communication about COVID‐19 risks and guidelines during the pandemic.MethodsParticipants were Canadian dentists (N = 644) recruited through the email roster of nine provincial dental associations or regulatory bodies. This qualitative analysis was nested within a prospective longitudinal cohort study in which data were collected using online questionnaires at regular intervals from August 2020 to November 2021. To address the objective reported in this paper, a conventional qualitative content analysis method was applied to responses to three open‐ended questions included in the final questionnaire.ResultsParticipants encountered challenges and frustrations amid the COVID‐19 pandemic, grappling with diverse regulations and communications from dental bodies. While some bodies offered helpful guidance, many participants felt the need for improved communication on guidelines. Dentists urged for expedited, clearer and more frequent updates, expressing difficulty in navigating overwhelming information. Negative views emerged on the vague and unclear communication of COVID‐19 guidelines, contributing to confusion and frustration among participants.ConclusionAs COVID‐19 persists and in planning for future pandemics, these experiential findings will help guide regulatory bodies in providing clear, timely and practical guidelines to protect the health and safety of dentists, dental staff, patients and the public.
Factor structure of the alcohol expectancies questionnaire among adolescents in rural Ghana
Authors: Weobong Benedict , Monk Rebecca L. , Anyorikeya Maria , Qureshi Adam W. , Heim Derek 
Published: January 2024

AbstractIntroductionChildren's early experiences with alcohol inform the development of alcohol‐related beliefs which are known to predict alcohol consumption during the critical stage of adolescence. Yet, there has been considerably less research into these alcohol‐related cognitions in low‐ and middle‐income countries (LMIC) and existing measures of these beliefs are highly reflective of Western contexts, which may not be fully appropriate for use in LMICs. The aim is to ascertain the construct validity of the Alcohol Expectancies Questionnaire (AEQ) in a non‐Western sample.MethodsA cross‐sectional diagnostic accuracy study involving 500 adolescents aged 10 to 18 years randomly selected from the database of the Navrongo Health and Demographic Surveillance System. Participants were administered the locally back translated version of the 34‐item AEQ. Confirmatory factor analysis using the lavaan package in R was conducted to generate indices for the factor structure of the AEQ.ResultsConfirmatory factor analyses showed that while groupings of positive and negative expectancies were similar to those observed when expectancies have been assessed previously in Western studies, these formed a single ‘alcohol expectancy’ factor. Questions relating to positive tension reduction and negative physical expectancies showed inconsistent responses in this study.Discussion and ConclusionsCommonly used tools for the assessment of alcohol expectancies may not be suitable for use in Ghana, possibly owing to their development and validation in Western contexts. These findings have implications for the assessment of alcohol‐related beliefs in LMIC settings and begin to map out a research agenda to develop more contextually and culturally attune alcohol assessments.
Studying serum neurofilament light chain levels as a potential new biomarker for small fiber neuropathy
Authors: Baka Panoraia , Steenken Livia , Escolano‐Lozano Fabiola , Steffen Falk , Papagianni Aikaterini , Sommer Claudia , Pogatzki‐Zahn Esther , Hirsch Silke , Protopapa Maria , Bittner Stefan , Birklein Frank 
Published: January 2024

AbstractBackground and purposeDiagnosing small fiber neuropathies can be challenging. To address this issue, whether serum neurofilament light chain (sNfL) could serve as a potential biomarker of damage to epidermal Aδ‐ and C‐fibers was tested.MethodsSerum NfL levels were assessed in 30 patients diagnosed with small fiber neuropathy and were compared to a control group of 19 healthy individuals. Electrophysiological studies, quantitative sensory testing and quantification of intraepidermal nerve fiber density after skin biopsy were performed in both the proximal and distal leg.ResultsSerum NfL levels were not increased in patients with small fiber neuropathy compared to healthy controls (9.1 ± 3.9 and 9.4 ± 3.8, p = 0.83) and did not correlate with intraepidermal nerve fiber density at the lateral calf or lateral thigh or with other parameters of small fiber impairment.ConclusionSerum NfL levels cannot serve as a biomarker for small fiber damage.
A systematic review and meta‐analysis of the efficacy of platelet‐rich plasma products for treatment of equine joint disease
Authors: Peng Cong , Yang Luo , Labens Raphael , Gao Yu , Zhu Yiping , Li Jing 
Published: January 2024

AbstractBackgroundEquine joint disease including septic arthritis (SA) and osteoarthritis (OA) is a critical cause of equine lameness. Platelet‐rich plasma (PRP) is one of the most popular regenerative therapies to treat equine OA, even SA, but the evidence in support of the treatment is conflicting.ObjectivesThe aim of the study was to systematically review the current evidence on PRP products used for SA and OA, as well as the efficacy of PRP products as treatment for OA on the basis of a meta‐analysis of the available literature.Study designSystematic review and meta‐analysis.MethodsA systematic search of relevant databases (PubMed, Web of Science, Scopus) was performed to identify studies published from 2013 to 2023, in accordance with the PRISMA guidelines. Randomised controlled trials, non‐randomised trials and controlled laboratory studies that used at least one type of PRP products were included. Dichotomous outcomes were presented using odds ratios (ORs) and 95% confidence intervals (95% CIs).ResultsA total of 21 publications were identified in the systematic review and 5 of them in the meta‐analysis. These publications involved various types of PRP products and reported different outcomes. Although most of the studies were associated with a high risk of bias, the overall estimated effect was consistent with a significant improvement in the PRP products treatment group compared with the control group (OR: 15.32; 95% CI: 3.00–78.15; p < 0.05). There was a significant improvement in clinical performance outcomes between the groups (OR: 36.64; 95% CI: 3.69–364.30; p < 0.05).ConclusionPRP products as intra‐articular treatment are likely efficacious for treatment of equine OA and have potential for treating SA. These conclusions might be affected by the limited number of randomised controlled studies and high variability of different types of PRP products. To better evaluate the efficacy of PRP, a widely recognised classification system and the utilisation of randomised, blinded, equivalency or non‐inferiority trials are required.
The effect of political alignment on street‐level bureaucrat job satisfaction and motivation
Authors: Piotrowska Barbara Maria 
Published: January 2024

AbstractThis paper examines how political alignment influences the job satisfaction and motivation of street‐level bureaucrats (SLBs)—low‐level frontline workers implementing policies while interacting directly with the public. I argue that political alignment affects SLBs' job satisfaction and motivation to work harder through three theoretical channels: (1) policy‐driven effects, particularly demoralization from implementing disagreeable policies; (2) spill‐over effects from other policy areas; and (3) political person‐organization fit effects, which result in decreased pride in their employer. Using the International Social Survey Program data from 34 countries, and panel data from the German Socio‐Economic Panel, tracking 880 respondents over 30 years, I find a robust link between political alignment and job satisfaction and motivation, and evidence for all three links. These findings underscore the importance of considering political alignment as a key factor in understanding and improving street‐level bureaucrat performance beyond policy‐specific and client‐specific contexts.
Masstige buyers: Profile, perceived luxury values and purchase intentions
Authors: Al‐Issa Nermain , Kwiatek Piotr , Dens Nathalie 
Published: January 2024

AbstractAs the term masstige has only recently emerged, the literature on masstige branding and marketing remains relatively limited. More research is needed to understand better the distinct profiles of masstige and luxury buyers, their perceived luxury values and purchasing motives. Our study, involving 601 UK consumers, aims to address this gap. Based on consumers' self‐reported prior experiences with masstige and luxury brands, we identify four distinct consumer segments: Premium luxury Buyers, Masstige Buyers, Buyers of Both Premium luxury and Masstige, and Non‐luxury buyers who have not previously purchased either Premium luxury or Masstige brands. We then explore each segment's socio‐demographic profiles, their luxury value perceptions, and how these values influence their purchase intentions. Individuals across diverse income levels, spanning low, middle, and high, fall into four distinct segments with unique luxury perceptions. Premium luxury buyers are characterized as socially oriented perfectionists, while masstige buyers align with hedonic materialism. Buyers of both seek self‐distinction. Non‐luxury buyers spire for group conformity, prioritizing experiential enjoyment when given the opportunity. Furthermore, our study offers a practical value‐based positioning framework and a roadmap for brands looking to expand their target markets. This framework can be a valuable resource for luxury marketers seeking to refine their positioning and better align with the values that resonate with their target audiences.
The Access Paradox: unmet healthcare needs among young adults in Alberta – an exploratory study of the systemic ironies in healthcare accessibility
Authors: Rao Sandy 
Published: January 2024

AbstractThere is a pressing need to investigate how young adults' access to healthcare, including how delayed help‐seeking behaviours and the complexities inherent in traditional healthcare systems, exacerbate the challenges they face. This study explores the associations among functional impairments, self‐perceptions of health, anxiety and mood disorders and their potential relationship to unmet healthcare needs among young adults aged 20–29. This cross‐sectional study used data from the 2017–2018 Canadian Community Health Survey to analyse a sample of 1636 young adults from Alberta, Canada. Central to the analysis was the application of conceptual framework of access to health care, as it offers a comprehensive view of healthcare access. Among the sampled young adults, 7.0% reported unmet healthcare needs. Self‐perceived unmet healthcare needs were significantly associated with anxiety disorders, functional impairments—specifically cognitive and social skills, and self‐perceptions of poor or fair mental health, as opposed to those perceiving their mental health as excellent. Multivariable analyses incorporating extraneous variables were not statistically significant, emphasising the critical role of systemic and structural factors in healthcare access. The study presents preliminary insights into the intricate dynamics shaping unmet healthcare needs. These insights can guide future research and practice advances, particularly in developing targeted interventions that effectively reduce healthcare disparities and enhance access to healthcare services for young adults.