bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electron ionization mass spectral fragmentation of C19 isoprenoid aldehydes and carboxylic acid methyl and trimethylsilyl esters
Authors: Rontani J.‐F. , Aubert C. 
Published: January 2005

AbstractThe electron ionization (EI) mass spectra of saturated and α,β‐unsaturated C19 isoprenoid aldehydes and carboxylic acid methyl and trimethylsilyl esters are reported. Different pathways are proposed in order to explain the main fragmentations observed. The conjugated double bond migrates more or less readily before γ‐hydrogen rearrangement according to the structure of the considered compound. Configurations of the double bond of α,β‐unsaturated C19 isoprenoid aldehydes and fatty acid methyl and trimethylsilyl esters can be easily determined thanks to the peaks at m/z 97, 127 and 185, respectively, which are much more abundant in the mass spectra of the Z isomers owing to the formation of a cyclic ion. In the case of trimethylsilyl esters, subsequent fragmentation of the cyclic ion at m/z 185 affords two other diagnostic ions at m/z 95 and 169. Copyright © 2003 John Wiley & Sons, Ltd.
A matrix‐assisted laser desorption/ionization mass spectrometry study of the non‐enzymatic glycation products of human globins in diabetes
Authors: Lapolla A. , Tubaro M. , Fedele D. , Reitano R. , Aricò N. C. , Ragazzi E. , Seraglia R. , Vogliardi S. , Traldi P. 
Published: January 2005

AbstractThe molecular species present in globins from healthy and diabetic subjects with and without chronic complications have been analyzed by matrix‐assisted laser desorption/ionization mass spectrometry. The technique demonstrated the presence of glycated and glyco‐oxidated species of both α‐ and β‐globins. Their abundances show a good linear relationship with respect to HbA1c values and with the mean daily plasma glucose levels over the 6 weeks preceding the investigation. Interestingly, slightly different behaviour is observed in the data from patients with and without chronic complications; the plots of HbA1c vs. the abundance of glycated and glyco‐oxidated species show different slopes and different intercepts with the y‐axis. To investigate this aspect the mean abundances of glyco‐oxidated species from healthy subjects and from diabetic patients with and without complications were calculated. Higher values were found for the two last sets of samples, but no significant difference was found between them. These data could indicate different individual proclivities to oxidation and/or different oxidation kinetics related to behavioural and environmental factors. Copyright © 2004 John Wiley & Sons, Ltd.
A simple method for the characterization and quantification of soy isoflavone metabolites in the serum of MMTV‐Neu mice using high‐performance liquid chromatography/electrospray ionization mass spectrometry with multiple reaction monitoring
Authors: Morandi Sheila , Locati Daniela , Ferrario Francesca , Chiesa Giulia , Arnoldi Anna 
Published: January 2005

AbstractIn a study aimed at elucidation of the possible role of dietary phytoestrogens in the growth of breast cancer, it was necessary to develop a convenient, accurate, and reproducible method for the characterization and quantification of isoflavone metabolites in the serum of MMTV mice that were fed diets containing different amounts of these polyphenols. The analytical method is based on liquid chromatography with mass spectrometry in multiple reaction monitoring mode, using deuterated genistein as internal standard. The identified metabolites were genistein and dihydrodaidzein (DHD); their average concentrations in serum were 0.71 and 0.21 μM, respectively, for animals fed 1.5 mg/day of genistein and 0.7 mg/day of daidzein. This method assured a limit of quantification of 0.04 μM for genistein and 0.08 μM for DHD, and a limit of detection of 0.018 μM for genistein and 0.035 μM for DHD. The coefficient of variation was 8.9% for genistein and lower than 15% for DHD. This study provides the first data indicating the presence of dihydrodaidzein in serum of mice fed diets containing soy proteins. Copyright © 2004 John Wiley & Sons, Ltd.
Determination of inorganic chlorine stable isotopes by continuous flow isotope ratio mass spectrometry
Authors: Shouakar‐Stash Orfan , Drimmie Robert J. , Frape Shaun K. 
Published: January 2005

AbstractChlorine stable isotope analyses of inorganic samples were conducted using continuous flow isotope ratio mass spectrometry (CF‐IRMS) coupled with gas chromatography (GC). Inorganic chloride was precipitated in the form of silver chloride (AgCl) by using silver nitrate in a standard methodology. Chlorine stable isotope analysis was carried out on methyl chloride (CH3Cl) after converting AgCl into CH3Cl by reacting it with methyl iodide (CH3I). The reaction between AgCl and CH3I took place in 20 mL size vials. Addition of CH3I was performed in a glove bag under helium flow. An Agilent 6890 gas chromatograph equipped with a CTC Analytics CombiPAL autosampler and a DB‐5MS 60 m column was used to separate CH3Cl from CH3I. This new technique uses samples as small as 0.2 mg of AgCl (1.4 μmol of Cl−). The chlorine stable isotope analysis using continuous flow technology showed excellent precision and accuracy. The internal precision using pure CH3Cl gas is better than ±0.04‰ (±STDV). The external precision using seawater standard is better than ±0.07‰ (±STDV) for n = 12. Moreover, the sample analysis time is much shorter and many more samples can be analyzed in one day than by using the conventional off‐line techniques. Copyright © 2004 John Wiley & Sons, Ltd.
A method for routine quantitation of urinary 8‐hydroxy‐2′‐deoxyguanosine based on solid‐phase extraction and micro‐high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry
Authors: Sabatini Laura , Barbieri Anna , Tosi Marco , Roda Aldo , Violante Francesco Saverio 
Published: January 2005

Abstract8‐Hydroxy‐2′‐deoxyguanosine (8OHdG), one of the major oxidative DNA lesions induced by radical agents, is commonly used as a biomarker for oxidative stress, nowadays preferably in urine. In the absence of a commercially available internal standard a micro‐high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (micro‐HPLC/ESI‐MS/MS) method, suitable for routine analysis of 8OHdG in human urine using external calibration, was developed. Evaluation of the matrix effect showed that the method allows highly sensitive and accurate quantitation despite the absence of an internal standard. HPLC analysis was performed using gradient elution at a flow rate of 10 μL min−1 using a capillary reversed‐phase column and an injection volume of 0.5 μL, with detection of 8OHdG in positive multiple reaction monitoring (MRM) mode. The absolute limit of detection was 0.35 fmol using m/z 168 as a quantifier (fragment) ion. A linear (R2 > 0.999) calibration curve in urine was obtained over a range 0.2–10 ng mL−1. This method is about 20 times more sensitive than previously described procedures, and is characterized by high accuracy (mean 90%) and good reproducibility (RSD < 10%). The optimized method was applied to determination of 8OHdG in 18 urinary samples derived from three healthy volunteers. 8OHdG urinary excretion ranged from 3.0–7.9 μg/day, and a large intra‐individual variation was found. This method, which effectively circumvents the need for isotopically labeled 8OHdG (internal standard), is suitable for routine monitoring of exposure to DNA‐damaging factors in a large number of subjects. Copyright © 2004 John Wiley & Sons, Ltd.
Characterization of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb by high‐performance liquid chromatography/electrospray ionization mass spectrometry
Authors: Schütz Katrin , Kammerer Dietmar R. , Carle Reinhold , Schieber Andreas 
Published: January 2005

AbstractPhenolic acids and flavonoids were extracted from a dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb juice and characterized by high‐performance liquid chromatography/electrospray ionization mass spectrometry. Among the 43 compounds detected, 5 mono‐ and dicaffeoylquinic acids, 5 tartaric acid derivatives, 8 flavone and 8 flavonol glycosides were characterized based on their UV spectra and their fragmentation patterns in collision‐induced dissociation experiments. The predominant compound was chicoric acid (dicaffeoyltartaric acid). Furthermore, several caffeoylquinic acid isomers were distinguished in dandelion extracts for the first time by their specific mass spectral data. The present study reveals that even more quercetin glycosides were found in dandelion than hitherto assumed. The occurrence of di‐ and triglycosylated flavonoids in particular has not yet been described. This paper marks the first report on HPLC‐DAD/ESI‐MSn investigations of phenolic compounds in dandelion. Copyright © 2004 John Wiley & Sons, Ltd.
Ketosteroid profiling using Girard T derivatives and electrospray ionization tandem mass spectrometry: direct plasma analysis of androstenedione, 17‐hydroxyprogesterone and cortisol
Authors: Johnson David W. 
Published: January 2005

AbstractA function of concentrations of three ketosteroids, [(androstenedione + 17α‐hydroxyprogesterone)/cortisol], has been shown to provide better discrimination than the concentration of 17α‐hydroxyprogesterone alone, for the diagnosis of the inherited disease congenital adrenal hyperplasia (CAH). A rapid, direct infusion electrospray ionization tandem mass spectrometry (ESI‐MS/MS) method has been developed to measure this ratio in plasma (100 μL). Sensitivity and specificity result from the preparation of mono‐Girard T derivatives. Androstenedione, 17α‐hydroxyprogesterone and cortisol are individually quantitated by isotope dilution. Excellent statistical correlation with radioimmunoassay (RIA) results for the analysis of 17α‐hydroxyprogesterone is demonstrated. The value of the ratio function of the three ketosteroids is 0.01–0.16 for normal controls (n = 26) and 1.83–18.7 for patients with severe CAH (n = 4). Profiles of ketosteroids derivatized with Girard T from the plasma of a child with severe CAH, generated by ESI‐MS/MS analysis, are shown. Neutral loss scans for 29.5 and 59 Da assist in the identification of the ketosteroids. Copyright © 2004 John Wiley & Sons, Ltd.
State of the Art in Burn Treatment
Authors: Atiyeh Bishara S. , Gunn S. William , Hayek Shady N. 
Published: January 2005

AbstractOptimal treatment of burn victims requires deep understanding of the profound pathophysiological changes occurring locally and systemically after injury. Accurate estimation of burn size and depth, as well as early resuscitation, is essential. Good burn care includes also cleansing, debridement, and prevention of sepsis. Wound healing, is of major importance to the survival and clinical outcome of burn patients. An ideal therapy would not only promote rapid healing but would also act as an antiscarring therapy. The present article is a literature review of the most up‐to‐date modalities applied to burn treatment without overlooking the numerous controversies that still persist.
Gallstone Disease after Extended (D2) Lymph Node Dissection for Gastric Cancer
Authors: Akatsu Tomotaka , Yoshida Masashi , Kubota Tetsuro , Shimazu Motohide , Ueda Masakazu , Otani Yoshihide , Wakabayashi Go , Aiura Koichi , Tanabe Minoru , Furukawa Toshiharu , Saikawa Yoshiro , Kawachi Shigeyuki , Akatsu Yukako , Kitajima Koichiro Kumai Masaki 
Published: January 2005

AbstractFew studies have reported the incidence and clinical outcomes of gallstone disease after extended (D2) lymph node dissection for gastric cancer. The present study was designed to retrospectively compare limited (D1) and D2 dissections in terms of gallstone formation, presentation of gallstones, and surgery for gallstone disease. A total of 805 Japanese gastric cancer patients (595 male, 210 female) who underwent curative resection with D1 (n = 490) or D2 (n= 315) dissection were retrospectively reviewed. Of those subjects followed for 70.5 ± 44.3 months (range: 2‐196 months), 102 (12.7%) developed gallstones. The incidence of gallstone formation was higher in the D2 group than in the D1 group (17.8% vs. 9.4%, p= 0.001). The interval between gastrectomy and detection of gallstones was shorter in the D2 group than in the Dl group (18.8 ± 11.4 months vs. 29.4 ± 18.3 months, p = 0.002). Of those with gallstones followed for 48.0 ± 28.6 months (range: 1‐158 months), 74 (72.5%) remained asymptomatic, and 15 (14.7%) experienced mild biliary pain. Thirteen patients (12.7%) developed recurrent biliary pain (n = 3) or biliary complications (n = 10; 6 acute cholecystitis, 3 obstructive jaundice, and 1 cholangitis), and required surgical treatment. Surgery was more frequently sought in the D2 group than in the D1 group (19.5% vs. 4.3%, p = 0.033). In conclusion, patients with D2 dissection developed gallstones more frequently and earlier than patients with D1 dissection. Of those with gallstones, patients with D2 dissection required surgery more often than patients with D1 dissection. A closer follow‐up should be mandatory for gallstone disease after D2 dissection, but further studies are needed before generalizations can be made.
Predictive Value of Procalcitonin for the Diagnosis of Bowel Strangulation
Authors: Ayten Refik , Dogru Osman , Camci Cemalettin , Aygen Erhan , Cetinkaya Ziya , Akbulut Handan 
Published: January 2005

AbstractIntestinal obstruction is responsible for 3% of admissions to hospital emergency surgical departments, but it is difficult to distinguish simple obstruction from strangulation. Simple criteria for this distinction are sought. In this experimental study, procalcitonin, a known marker of bacterial inflammation, was used to detect strangulation. The predictive value of procalcitonin for small bowel strangulation was evaluated. Thirty male New Zealand rabbits (mean weight: 3.0 kg) were divided into three groups. In the first (control) group, only laparatomy was performed. In the second group, simple obstruction was created by ligating a 10‐cm distal ileum segment. In the third group, distal strangulation was created by ligating a 10‐cm distal ileum segment with the mesentery. Blood (1 cc) was taken from the right auricular vein of each animal for measuring the procalcitonin level. In both the control group and the simple obstruction group the procalcitonin levels were normal. In the strangulation group, elevation of procalcitonin was detected after 30 minutes, and the elevation was statistically significant at 120th minute compared with the control and simple obstruction groups. In patients with small bowel obstruction, measurement of procalcitonin levels is easy to perform and can be used in the follow‐up. A more extensive clinical study is needed to evaluate the accuracy of the test as a marker.
Addition of Rectus Sheath Relaxation Incisions to Emergency Midline Laparotomy for Peritonitis to prevent Fascial Dehiscence
Authors: Marwah Sanjay , Marwah Nisha , Singh Mandeep , Kapoor Ajay , Karwasra Rajender Kumar 
Published: January 2005

AbstractThe incidence of fascial dehiscence and incisional hernia after two methods for abdominal wound closure (rectus sheath relaxation incisions and conventional mass closure) was studied in a randomized prospective clinical trial in a consecutive series of 100 patients undergoing midline laparotomy for peritonitis. The two groups were well matched for etiologies of peritonitis, the surgical procedures performed, and the presence of known risk factors for fascial dehiscence. Fifty patients each were randomized either to the conventional continuous mass closure procedure or the rectus sheath relaxation incision technique (designed to increase wound elasticity and decrease tension in the suture line) using identical polypropylene sutures. The incidence of postoperative complications such as duration of ileus, chest infection, and wound infection were not statistically different between the two groups. The intensity of postoperative pain in the rectus sheath relaxation incision group was significantly less. The incidence of wound hematoma was significantly increased in the rectus sheath relaxation incision group. The incidences of fascial dehiscence (16% vs,28%; p < 0.05) and incisional hernia (18% vs, 30%; p < 0.05) were significantly lower after rectus sheath relaxation incisions compared to conventional mass closure. Closure of the midline laparotomy wound in cases of peritonitis using the rectus sheath relaxation technique is safe and less painful, provides increased wound elasticity and decreased tension on the suture line, and significantly decreases the incidence of wound dehiscence.
Impact of Age on Quality of Life in Patients with Rectal Cancer
Authors: Schmidt Christian E. , Bestmann Beate , Kuchler Thomas , Longo Walter E. , Kremer Bernd 
Published: January 2005

AbstractSome studies indicate that age at the time of surgery has a general effect on outcomes. The impact of age on the quality of life (QOL) of patients with rectal cancer, however, has not been investigated. The present study was conducted to address this issue. Over a 5‐year period the European Organization for Research and Treatment of Cancer (EORTC)‐QLQ‐C‐30 and a tumor‐specific module were prospectively administered to patients before surgery, at discharge, and at 3, 6, 12, and 24 months postoperatively. Comparisons were made between age groups. A total of 519 patients participated in the study. QOL data were available for 253 patients. Significant differences were observed only between patients aged 69 years and younger (≤69 years) (169/253) and those aged 70 years and older (≥70 years) (85/253). Physical and role functioning was better for patients ≤69 years; patients ≥70 years suffered from increased pain and fatigue. Younger patients had more difficulty with sexual enjoyment, and over time sexual strain was worse for patients aged ≥70 years during the early postoperative period but improved, whereas patients aged ≤69 years had increasing levels of strain over time. The findings in this study confirmed that QOL is dynamic over time and that age has an impact on QOL and sexuality. Patients aged ≥70 years are affected by impaired physical functioning, global health, and fatigue, whereas increased treatment strain during the early postoperative period improves over time. Patients aged ≤69 years experience increased strain because of impaired sexual function.
Hemorrhage after Duodenopancreatectomy: Impact of Neoadjuvant Radiochemotherapy and Experience with Sentinel Bleeding
Authors: Turrini Olivier , Moutardier Vincent , Guiramand Jerome , Lelong Bernard , Bories Erwan , Sannini Antoine , Magnin Valerie , Viret Frederic , Blache Jean‐Louis , Giovannini Marc , Delpero Jean‐Robert 
Published: January 2005

AbstractPostoperative hemorrhage (PH) after duodenopancreatectomy (DP) is frequently lethal. The aim of this study was to delineate guidelines of management. Between August 1994 and July 2003, 172 patients underwent DP for cancer. Altogether, 26 patients were subjected to an institutional protocol (IP) with standard‐dose chemoradiation (CRT) and 4 patients to an extrainstitutional protocol (EIP) with high‐dose CRT. Sixteen patients (9.3%) were reoperated for PH. Hemorrhage occurred in 23% of irradiated patients (4 EIP, 3 IP) and in 6% of nonirradiated patients [confidence interval (CI) 1.8‐6.5]. Pancreatic leak occurred in nine patients with PH (56%). Sentinel bleeding (SB) was noted in eight patients (50%) with a mean delay of 10 days after DP. Overall mortality after hemorrhage was 56%. Morality rates of patients with EIP or IP were, respectively, 100% and 0%. Mortality rates of patients with or without SB were similar. Mortality rates of axial bleeding (hepatic artery, mesenteric vessels) or lateral bleeding (pancreas remnant, splenic vessels) were, respectively, 88% and 25% (CI 1.6‐8.6). Completion of pancreatectomy was achieved in 75% without rebleeding. Preoperative high‐dose CRT increased the risk of fatal PH. Because SB occurs before massive hemorrhage, prompt reoperation could reduce mortality. Completion of pancreatectomy was essential during reintervention. Axial bleeding supports high mortality. Moving to the left, the pancreatojejunostomy could avoid contact of pancreatic juice with axial vessels in the case of pancreatic leakage. Ligating the gastroduodenal artery during DP had to leave a stump of around 1 cm to facilitate hemorrhage control without ligating the common hepatic artery.
Occult Intrabiliary Rupture of Hydatid Cysts in the Liver
Authors: Hamamci Enver Okan , Besim Hasan , Sonisik Muhittin , Korkmaz Atila 
Published: January 2005

AbstractThe aim of this study was to investigate the effects of various surgical modalities directed at the cavity of hydatid cysts in patients with occult intrabiliary rupture. In this respect, 324 patients with hydatid cyst of the liver operated on during 1983‐2003 were analyzed; among them, 39 patients with occult intrabiliary rupture were included in the study. Clinical symptomatology, physical examination, laboratory findings, results of imaging studies, the localization and size of the cyst, and operative findings were reviewed. Twelve patients had complications, and there was no mortality. The most common complication was bile fistula. The average postoperative hospital stay was 7.0 ± 3.8 days for patients with omentoplasty and 6.0 ± 2.5 days for those who underwent cavitary drainage. For patients who do not have bile‐stained cystic fluid, the utilization of scolicidal agents is appropriate. Although the opening of the duct is sutured when it is identified, the risk of biliary fistula is not clearly correlated with this approach. In such cases, omentoplasty provides a good alternative to cavitary drainage.
A Critical Evaluation of Hepatic Resection in Cirrhosis: Optimizing Patient Selection and Outcomes
Authors: Emond Jean C. , Samstein Benjamin , Renz John F. 
Published: January 2005

AbstractHepatic resection has long been the mainstay of treatment of primary liver cancers, particularly hepatocellular carcinoma (HCC). Because of the high incidence of cirrhosis in patients with HCC, the use of resection was initially limited by the ability of the cirrhotic liver to sustain the surgical insult and the mass reduction. Today, hepatectomy in cirrhosis is undergoing a remarkable evolution. Although surgical and anesthetic improvements have increased the safety of this option, the rapid development of alternative therapies has decreased the need for it. Local excision for small HCC is likely to be replaced by image‐guided, percutaneous ablative techniques. Furthermore, total replacement of a cirrhotic liver may be a more effective long‐term cure than resection. Unquestionably, resection remains the optimal approach for patients with large tumors and healthy underlying liver function. The role of rapidly evolving new approaches will remain the subject of intensive inquiry in the years to come. In this report, we have attempted to clarify current practice with respect to the evaluation, selection, and technique of resection in cirrhosis, and identify areas of active inquiry.