bluuebunny/crossref_metadata_2025_split huggingface dataset:
The characteristic analysis of TaTDF1 reveals its function related to male sterility in wheat (Triticum aestivum L.)
Authors: Shan Sicong , Tang Peng , wang Rui , ren Yihang , Wu Baolin , Yan Nuo , Zhang Gaisheng , Niu Na , Song Yulong 
Published: November 2023

Abstract

Background
The male sterile lines are an important foundation for heterosis utilization in wheat (Triticum aestivum L.). Thereinto, pollen development is one of the indispensable processes of wheat reproductive development, and its fertility plays an important role in wheat heterosis utilization, and are usually influencing by genes. However, these key genes and their regulatory networks during pollen abortion are poorly understood in wheat.
Results
The rice gene TDF1, a member of the R2R3-MYB family, is crucial for early tapetum development and pollen grain fertility. TaTDF1, an ortholog of rice gene TDF1, was identified in wheat, with an average bolting time of 33 d in TaTDF1-OE Arabidopsis plants. The anthers of TaTDF1-OE plants appeared red. TaTDF1-EAR plants were shrunken and small, showing a blue-green color after Alexander's staining. The TaTDF1 interacting protein (TaMAP65) was confirmed using Yeast Two-Hybrid Assay and Bimolecular-Fluorescence Complementation experiments. The results showed that downregulated expression of TaTDF1 and TaMAP65 could cause anthers to be smaller and shrunken, leading to pollen abortion in TaTDF1 wheat plants induced by virus-induced gene-silencing technology. The expression pattern of TaTDF1 was influenced by TaMAP65.
Conclusions
Thus, systematically revealing the regulatory mechanism of wheat TaTDF1 during anther and pollen grain development may provide new information on the molecular mechanism of pollen abortion in wheat.
Analysis of Sea surface temperature pattern, variability and their teleconnection with rainfall dynamics over their Gulf of Guinea
Authors: Ideki Oye , Lupo A. R 
Published: November 2023

Abstract

Spatial pattern and variability of sea surface temperature SST and its influence on seasonal rainfall variation in the Gulf of Guinea (GOG) was examined in this study. SST data of 50 years (1970–2022) from ERA5 and NOAA CPC at 0.250 x 0.250 and 0.50 x 0.50 resolution was obtained from specific locations namely longitudes 20 °W and 10 °E and latitudes 15 °N and 2 °N. Twelve rainfall gridded stations distributed throughout Gulf of Guinea (GoG) were used to characterize the Rainfall-SST variability across the region. To investigate the relationship between the rainfall anomalies in GoG on seasonal, inter-annual and decadal scales, cross correlation analyses and composites were used. IDW from the ArcGIS Spatial Analyst Tool, Ferret and CDO were used to obtain the seasonal and decadal rainfall and SST maps and statistical analysis over the region of study. The result of the decadal analysis of SST variability from 1970–1980,1980–1990, 1990–2000,2000–2010,2010–2020 and 2022 indicate that SST was highest during the period of 2010–2020 at 28.91oC and fluctuated between 1970–1980 (28.49oC) and 1980–1990 (28.08OC) While the seasonal pattern of SST showed marked variability with March-April and May recording 29.34oC(MAM) and the lowest been in June-July-August(JJA) at 28.71oC. In terms of decadal analysis of rainfall, the period 2010–2020 recorded the highest amount of rainfall along the coast (3,145.5mm-3,928.3mm while 1970–1980 recorded the lowest amount of rainfall (2,650-3.310mm. To assess the teleconnection between of SST and precipitation dynamics, SST values were plotted against seasonal rainfall in nine stations namely Abijan ,Accra, Guinea, Conakry, Doula, Freetown, Lagos, Lome and Monrovia showed positive correlation except Doula. The spatial pattern of seasonal rainfall climatology categorized in to DJF,MAM, JJA and SON reveals that JJA and SON produced 80% of rainfall in the Coastal GOG followed by MAM. The study affirmed that warm and cold tongue exist in the GOG alongside positive teleconnection and that the spatial variability of SST observed in this study corresponds positively with the decadal and seasonal variability of rainfall
Multiple primary colon carcinomas with small cell neuroendocrine lung carcinoma: A case report and literature review
Authors: Gao Jian , Luo Zengyou , Ma Zhiming 
Published: November 2023

Abstract
Background
The incidence of multiple primary cancers is increasing with age and improved medical screening, but the simultaneous coexistence of solitary lung cancer and multiple colon cancers is rare.
Case demonstration
A 69-year-old male patient was admitted to the hospital with a physical examination that revealed a mass in the right lung and another in the hepatic flexure of the colon. Furthermore, a colonoscopy identified three masses in the colon. Therefore, the patient was diagnosed with a "pulmonary mass" along with a "colonic mass”. Following a comprehensive multidisciplinary discussion, the patient underwent a "lobectomy", and the postoperative pathology report indicated a "neuroendocrine tumor". The patient was admitted to the hospital again three months later due to abdominal pain and difficulty with bowel movements. He was diagnosed with multiple colon cancer and underwent surgery. The pathology report confirmed adenocarcinoma, and then it was confirmed to be a primary tumor originating from two different organs. Postoperative adjuvant CapeOx chemotherapy was performed after surgery. After two consecutive years of follow-up, no recurrence or metastasis of the tumor was observed.
Conclusion
Clinical efforts should not only focus on diagnosing one cancer but also consider the possibility of other diseases. The importance of a systematic approach to comprehensive diagnosis should be highlighted. Intraoperative colonoscopy and PET-CT have certain advantages in detecting MPCs. The treatment of MPCs should follow the principle of an individualized therapeutic approach. Tumors with a poor prognosis and higher risks should receive priority treatment.
Respirable fine particulate matter (PM2.5) aggravates cerebral ischemia-reperfusion injury in rats by inducing high expression of LOX-1/ NF-κB
Authors: Li Wei , Li Chang-qing , Zhou Peng-bo , Chen Yue-yang , Sun Hong-tao , Chai Er-qing 
Published: November 2023

Abstract
Objective:To investigate the mechanism of exacerbating cerebral ischaemia/reperfusion injury in rats after PM2.5 exposure.
Methods: Eight-week-old SD male rats were selected and randomly grouped into the following groups: control group, I/R group, I/R+PM2.5 low concentration group, I/R+PM2.5 medium concentration group, I/R+PM2.5 high concentration group, and the rats were given the appropriate interventions.The volume of cerebral infarction, the scores of neurological deficits at 24 hours and 48 hours postoperatively; the expression levels of LOX-1, NF-κB, ROS , SOD in brain tissues, and the expression levels of inflammatory factors (IL-1α, IL-1β, IL-18, TNF-α) in serum were observed in rats.
Results: Compared with the I/R group, short-term exposure to medium and high concentrations of PM2.5 increased the volume of cerebral infarction and aggravated the neurological impairment in ischemia-reperfusion rats (p<0.05), increased the expression levels of inflammatory factors (IL-1α, IL-1β, IL-18, TNF-α) in serum of the I/R rats (p<0.05), inhibited SOD, promoted the expression of ROS, and increased the oxidative stress injury. Moreover, moderate and high PM2.5 exposure significantly up-regulated the protein expression levels of LOX-1 and NF-κB in the brain tissue of ischemia-reperfusion rats (p<0.001).
Conclusion: Medium and high PM2.5 exposure increases inflammatory reactions and oxidative stress injury in ischemia-reperfusion rats, exacerbating cerebral infarct volume and neurological impairment. The neurological damaging effects of PM2.5 exposure in I/R rats may be related to its pro-inflammatory and pro-oxidative effects through up-regulation of LOX-1, NF-κB inflammatory mediators.
SiC generation mechanism in photovoltaic crystal pulling waste graphite infusion cylinder
Authors: Hu Yiyou , Chen Zhengjie , Zhang Yonghang , Chen Xiuhua , Ma Wenhui 
Published: November 2023

Abstract
Carbon etching and silicide deposition are common phenomena in furnaces during photovoltaic crystal pulling processes,both of which decrease Graphite components in the furnace service life and pollute silicon-based materials. SiC as one of the major silicides and carbon etching products whose generation mechanism is of practical significance. Therefore, this study took discarded graphite infusion cylinders with significantly different apparent morphologies as the research objects. Through systematic characterization and thermodynamic analysis, we investigated the physical and chemical reactions that occurred during the generation of SiC in various regions of a discarded graphite infusion cylinder. We derived the SiC delamination, infiltration, and phagocytosis mechanism to explain the formation of the apparent morphological characteristics of the waste graphite infusion cylinder. We also described Generation mechanism of SiC in graphite infusion cylinder during photovoltaic crystal pulling process to provide a theoretical basis for prolonging the service life of the graphite infusion cylinder and treating waste graphite infusion cylinder .
Frontal deficits and atrophy in a patient with neuroserpinosis detected by single-case voxel-based morphometry: A case report
Authors: Handa Hideo , Sugiyama Atsuhiko , Kaname Tadashi , Shigemoto Yoko , Sato Noriko , Hirano Shigeki , Nakagawa Yuki , Uzawa Akiyuki , Aotsuka Akiyo , Kuwabara Satoshi 
Published: November 2023

Abstract
Background: Neuroserpinosis is a rare genetic disorder characterized by progressive cognitive decline and myoclonic epilepsy, caused by pathogenic variants of SERPINI1. We reported a case of genetically confirmed neuroserpinosis with de novo H338R mutation in the SERPINI1, in which frontal deficits including inattention and disinhibition, and relevant atrophy in the vmPFC on brain MRI were observed in the early stage of the disease.
Case presentation: A 23-year-old Japanese man presented with progressive inattention and disinhibition over 4 years followed by myoclonic epilepsy. The whole-genome sequencing and filtering analysis showed de novoheterozygous H338R mutation in the SERPINI1, confirming the diagnosis of neuroserpinosis. Single-case voxel-based morphometry using brain magnetic resonance imaging obtained at the initial visit revealed focal gray matter volume loss in the ventromedial prefrontal cortices, which is presumed to be associated with inattention and disinhibition.
Conclusion: Frontal deficits including inattention and disinhibition can be the presenting symptoms of patients with neuroserpinosis. Single-case voxel-based morphometry may be useful for detecting regional atrophy of the frontal lobe in neuroserpinosis. Detecting these abnormalities in the early stage of disease may be key findings for differentiating neuroserpinosis from other causes of progressive myoclonic epilepsy.
Effects of energy director types on the interface morphology and tensile properties of ultrasonically welded CF/PAEK joints
Authors: Xiao Li , Li Binbin , Bu Hengchang , Gao Qiyu , Li Xiaodong , Zhan Xiaohong 
Published: November 2023

Abstract
This study investigates the impact of ultrasonic welding amplitudes and time on the properties of carbon fiber reinforced polyaryletherketone (CF/PAEK) composite joints. To enhance the performance of CF/PAEK ultrasonic welded joints, a hybrid energy director (ED) was proposed, which was composed of the interfacial microgroove and resin film or metal mesh. This study investigated the effect of different types of ED on the forming quality, shear failure load, and fracture interface microstructure of single lap joints made of CF/PAEK. The results indicated that the hybrid ED with resin film offers a distinct effect on enhancing the strength of ultrasonic welded joints. The hybrid ED with resin film essentially improves the tensile properties of the joint, with the strength and toughness increased by 35.8% and 174.3%, respectively. This strengthening effect is primarily attributed to the added resin film providing adequate interfacial resin. Sufficient resin is filled into the interior of the microgroove, ultimately forming a mechanical anchoring structure to strengthen the joint strength.
Enhancing Tea Disease Identification with Lightweight MobileNetV2
Authors: Li Zhilin , Li Yuxin , Yan Chunyu , Yan Peng , Li Xiutong , Yu Mei , Wen Tingchi , Xie Benliang 
Published: November 2023

Abstract
Plant diseases in tea trees can result in significant losses in both the quality and quantity of tea production. Regular monitoring can prevent the occurrence of large-scale diseases in tea plantations. However, existing methods face challenges such as a high number of parameters and low recognition accuracy, which hinder their application for monitoring tea gardens on edge devices. This paper presents a lightweight I-MobileNetV2 model for identifying diseases in tea leaves, with the goal of addressing these challenges. The proposed method embeds a coordinate attention mechanism module into the original MobileNetV2 network, enabling the model to accurately locate disease regions. Furthermore, a multi-branch parallel convolution module is employed to extract disease features across multiple scales, which improves the adaptability of the model to different disease scales. Then an automated pruning strategy is employed to compress the model and reduce computational complexity. The results indicate that algorithm proposed surpass the original MobileNetV2 by 1.91 percentage points with an average accuracy of 96.12% based on self-built tea disease dataset, the model parameters have been reduced by 40%, making it more suitable for practical application in tea garden environments.
Cross-border comparison of mycobiomes in Parmelia and Peltigera lichens from Turkey and South Korea
Authors: Yang Jiho , Woo Jung-Jae , Sesal Cenk , Gökalsın Barış , Açıkgöz Birkan , Başaran Tunahan Irmak , Kurtuluş Gamze , Hur Jae-Seoun 
Published: November 2023

Abstract

Lichens, traditionally considered as a simple partnership primarily between fungi and photobiont, are, in reality, complex ecosystems comprised of a multitude of microorganisms. Endolichenic fungi represent fungi residing within lichen thalli without causing any disease symptoms. Various factors, including the host lichen species and environmental conditions, can influence the structure of the lichen mycobiome. To investigate which factor, host genus or location, has a greater impact on the lichen mycobiome, we conducted a comparative analysis of mycobiomes withinParmeliaandPeltigeracollected from both Turkey and South Korea, using high-throughput sequencing. In terms of taxonomic diversity, the lichen mycobiome does not show significant differentiation based on host phylogeny or geographical distance, suggesting similar characteristics. Location differences exert a stronger influence on the lichen mycobiome than the host genus. We have identified the core mycobiomes ofParmeliaandPeltigera, and these two core mycobiomes share common fungal strains including basidiomycete yeast. Our study enhances our understanding of lichen mycobiomes, shedding light on their taxonomic diversity, the factors influencing their composition, and the core members and specialists within these intricate ecosystems.
Entrepreneurship cultivation and application modeling of artificial intelligence under the construction of "Three regions and One District" in Anhui Province
Authors: Jiang Leilei , Hu Pan , Dong Ke 
Published: November 2023

Abstract

As a double border area of eastern and central areas, anhui "duplicity", facing the national development strategy of multiple stacking, cultural historical heritage deep, science and technology innovation ability for many years in the country the first phalanx, transportation network access, the ecological environment is good, in the development of Yangtze river delta integration has obvious comparative advantage. Therefore, Anhui should take the initiative in the integration of the Yangtze River Delta and the high-quality development of the central region. The use and promotion of artificial intelligence technology is a profound educational change in innovation and entrepreneurship education, which better meets the requirements of the combination of theory and practice in innovation and entrepreneurship education, and has broad development prospects and great application value. This paper establishes a complete set of application modeling system of entrepreneurship training based on artificial intelligence technology. The experimental results show that the application model of entrepreneurial cultivation has good prediction performance, and the evaluation model can provide accurate information and decision support, which has a good application prospect.
Examining Physical Activity’s Impact on Delivery, Institution based cross- sectional study
Authors: Hailu Molla , Amsalu Hailemariam 
Published: November 2023

Abstract
Background
Although cesarean section is an essential maternal healthcare service, it has both maternal and neonatal adverse outcomes as compared to spontaneous vaginal delivery. Physical activities enhance the strength of the abdominal, diaphragm, and pelvic floor muscles, stretching the thigh and back leg adductors, all these help the women more easily turn to and keep their natural delivery positions. This might facilitate spontaneous vaginal delivery with a short duration of labor.
Objective
To assess the effect of degree of physical activity during pregnancy on mode of delivery and duration of labor.
Methods
A cross-sectional study was conducted in 150 women who gave birth at MizanTepi University Teaching Hospital (MTUTH), Ethiopia. Data was collected through face-to-face interviews using a structured questionnaire. Descriptive analysis, chi-square and t-test were conducted to check the presence of any association between the dependent and independent variables. A p-value of < 0.05 will be considered statistically significant.
Result
Our study found that 87% of women who engaged in vigorous physical activities had spontaneous vaginal deliveries. Of those women who have light physical activities, only 16.5% have spontaneous vaginal delivery. The duration of labor for women with vigorous and light physical activity was 5.2 ± 2.07 and 8.9 ± 2.8 hours, respectively.
Conclusion
Engaging in the recommended amount of aerobic exercise while pregnant under a doctor's supervision is crucial for reducing the duration of labor and the need for an operational delivery.
Development and Validation of Self-assessment Instrument to Measure the Digital Professionalism of Healthcare Professionals Using Social Media
Authors: Imran Shazia , Yasmeen Rahila , Mansoor Memoona 
Published: November 2023

Abstract

Background:The use of social media across the globe has risen incrementally. During the COVID-19 pandemic, these sites undeniably provided new avenues for professional networking but also led to a surge in cases of online misconduct. Professionalism instruments and scales do not assess the digital attitude and behavior of healthcare professionals (HCPs). The purpose of this study was to identify the domains of digital professionalism related to social media use and to validate a self-assessment instrument to assess the digital professionalism of HCPs using social media.MethodsAn instrument development multiphase mixed method study (exploratory sequential) was conducted in two phases: item development and qualitative content validation followed by validation of the instrument. Feedback was taken from 15 experts for qualitative content validation in phase 1. In phase 2, content validity was established through three rounds of modified Delphi. Validity evidence was collected for the content (content validity index), response process (cognitive interviews), internal structure (confirmatory factor analysis), and internal consistency (Cronbach’s alpha).ResultsThe 48-item preliminary instrument was reduced to a 28-item instrument with eight domains: self-anonymity, privacy settings, maintenance of boundaries and confidentiality, conflict of interest, accountability, respect for colleagues, and ethics. The content validity index of the scale was 0.91. The reliability and construct validity of the instrument was established by responses from 500 healthcare professionals from multiple hospitals. Confirmatory factor analysis showed a model with a goodness-of-fit index of 0.86, root mean square error of approximation of 0.06, and observed normed χ2of 2.7. The internal consistency through Cronbach's alpha α was 0.96.ConclusionThe digital professionalism self-assessment instrument (DP-SAI) has an appropriate level of content and measures the construct reliably. It can be used by medical doctors, dental clinicians, nurses, physiotherapists, and clinical pharmacists to self-assess and reflect on their social media practices. This will help to address these issues to enhance the quality of online communication through various social media platforms.
Targeting GD2-positive Refractory/Resistant Neuroblastoma and Osteosarcoma with Anti- CD3 x Anti-GD2 Bispecific Antibody Armed T cells
Authors: Yankelevich Maxim , Thakur Archana , Modak Shakeel , Chu Roland , Taub Jeffrey , Martin Alissa , Schalk Dana L. , Schienshang Amy , Whitaker Sara , Rea Katie , Lee Daniel W. , Liu Qin , Shields Anthony , Cheung Nai-Kong , Lum Lawrence G. 
Published: November 2023

Abstract
Background: Since treatment of neuroblastoma (NB) with anti-GD2 monoclonal antibodies provides a survival benefit in children with minimal residual disease and our preclinical study shows that anti-CD3 x anti-GD2 bispecific antibody (GD2Bi) armed T cells (GD2BATs) were highly cytotoxic to GD2+ cell lines, we conducted a phase I/II study in recurrent/refractory patients to establish safety and explore the clinical benefit of GD2BATs.
Methods: The 3+3 dose escalation study (NCT02173093) phase I involved 9 evaluable patients with NB (n=5), osteosarcoma (OST) (n=3), and desmoplastic small round cell tumors (DSRCT) (n=1) with twice weekly infusions of GD2BATs at 40, 80, or 160 x 106 GD2BATs/kg/infusion with daily interleukin 2 (300,000 IU/m2) and twice weekly granulocyte-macrophage colony stimulating factor (250 µg/m2). Phase II portion of the trial was conducted in patients with NB at the dose 3 level of 160 x 106 GD2BATs/kg/infusion but failed to enroll the planned number of patients.
Results: Nine of 12 patients in the phase I completed therapy. There were no dose limiting toxicities (DLTs). All patients developed mild and manageable cytokine release syndrome (CRS) with grade 2-3 fevers/chills, headaches, and occasional hypotension up to 72 hours after GD2BAT infusions. GD2-antibody associated pain was not significant in this study. The median OS for patients in the Phase I and limited Phase II was 18.0 and 31.2 months, respectively, whereas the combined OS was 21.1 months. There was a complete bone marrow response with overall stable disease in one of the phase I patients with NB. Ten of 12 phase II patients were evaluable for response: 1 had partial response. Three additional patients were deemed to have clinical benefit with prolonged stable disease. More than 50% of evaluable patients showed augmented immune responses to GD2+ targets after GD2BATs as measured by interferon-gamma (IFN-γ) EliSpots, Th1 cytokines, and/or chemokines.
Conclusions: Our study demonstrated safety of up to 160 x 106 cells/kg/infusion of GD2BATs. Combined with evidence for the development of post treatment endogenous immune responses, this data supports further investigation of GD2 BATs in larger Phase II clinical trials.