bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wellbeing and burnout among oncology fellows compared to prior fellows and current practicing oncologists: An ASCO State of Cancer Care in America (SOCCA) trend analysis.
Authors: Subbiah Ishwaria M. , Schenkel Caroline , Kirkwood Margaret Kelsey , Levit Laura A. , Shanafelt Tait D. 
Published: October 2023

47 Background: Burnout impacts oncology clinicians across their professional lifecycle. To better understand clinician wellbeing and the role of fellowship training, we characterized trends in oncology fellows’ burnout and perception of preparedness during training. Methods: As an ASCO SOCCA initiative, all medical oncology fellows taking the 2023 ASCO Medical Oncology In-Training Exam (ITE) were invited to complete an optional post-ITE professional wellbeing survey. Fellows were asked 1 item on fatigue (on a 0-10 Likert scale, 10 = “as bad as can be”), 1 item on satisfaction with work-life integration (WLI) and 2 Maslach Burnout Inventory (MBI) items on emotional exhaustion (EE) and depersonalization (DP), each scored on a 1-7 scale, >5 being high. Per convention, respondents with a high EE and/or DP were categorized as having burnout. 2023 ITE responses were compared to (1) 2013 ASCO Fellows Post-ITE survey (n=1345),1 (2) 2012-13 ASCO survey of practicing oncologists (n=1117),2 and (3) 2023 ASCO survey of practicing oncologists (n=328; 26% tagged as early career, using age <40 as proxy; 64% in academia). Results: 2231 fellows who took the ITE in 2023. Responses from 1568 first (33%), second (36%) and third year (31%) fellows who responded to at least one wellbeing item are included in this analysis. Compared to 10 years prior, oncology fellows in 2023 reported less burnout (20% in 2023 vs 34% in 2013, p<.001) and higher satisfaction with WLI (50% in 2023 vs 34% in 2013; p<.001). Fellows across both 2013 and 2023 studies reported higher satisfaction with WLI with advancing fellowship year (1-3). The 2013 analyses found no statistically significant difference in EE, DP or overall burnout between fellows and practicing oncologists. However, in 2023, the prevalence of high EE, DP and burnout was significantly lower in fellows vs both all and early career practicing oncologists (all p<.005, Table). Most (72%) fellows in 2023 felt their training is adequately preparing them to manage burnout in future practice. Conclusions: Oncology fellows in 2023 reported significantly less burnout and higher WLI satisfaction than fellows in 2013 and current practicing oncologists. Most fellows felt prepared to manage burnout in future practice. The higher prevalence of burnout in early career oncologists vs fellows suggests a gap between expectations of practice during training and the actual experience of oncology practice. (1) Shanafelt et al: JCO 32(27), 2014. (2) Shanafelt et al: JCO 32(11), 2014. [Table: see text]
Unraveling Vulnerabilities in Endocrine Therapy-Resistant HER2+/ER+ Breast Cancer
Authors: Bahnassy Shaymaa , Stires Hillary , Jin Lu , Tam Stanley , Mobin Dua , Balachandran Manasi , Podar Mircea , McCoy Matthew D , Beckman Robert A , Riggins Rebecca B 
Published: October 2023

Abstract
Breast tumors overexpressing human epidermal growth factor receptor (HER2) confer intrinsic resistance to endocrine therapy (ET), and patients with HER2/estrogen receptor–positive (HER2+/ER+) breast cancer (BCa) are less responsive to ET than HER2–/ER+. However, real-world evidence reveals that a large subset of patients with HER2+/ER+ receive ET as monotherapy, positioning this treatment pattern as a clinical challenge. In the present study, we developed and characterized 2 in vitro models of ET-resistant (ETR) HER2+/ER+ BCa to identify possible therapeutic vulnerabilities. To mimic ETR to aromatase inhibitors (AIs), we developed 2 long-term estrogen deprivation (LTED) cell lines from BT-474 (BT474) and MDA-MB-361 (MM361). Growth assays, PAM50 subtyping, and genomic and transcriptomic analyses, followed by validation and functional studies, were used to identify targetable differences between ET-responsive parental and ETR-LTED HER2+/ER+ cells. Compared to their parental cells, MM361 LTEDs grew faster, lost ER, and increased HER2 expression, whereas BT474 LTEDs grew slower and maintained ER and HER2 expression. Both LTED variants had reduced responsiveness to fulvestrant. Whole-genome sequencing of aggressive MM361 LTEDs identified mutations in genes encoding transcription factors and chromatin modifiers. Single-cell RNA sequencing demonstrated a shift towards non-luminal phenotypes, and revealed metabolic remodeling of MM361 LTEDs, with upregulated lipid metabolism and ferroptosis-associated antioxidant genes, including GPX4. Combining a GPX4 inhibitor with anti-HER2 agents induced significant cell death in both MM361 and BT474 LTEDs. The BT474 and MM361 AI-resistant models capture distinct phenotypes of HER2+/ER+ BCa and identify altered lipid metabolism and ferroptosis remodeling as vulnerabilities of this type of ETR BCa.
The shift to 3D growth during embryogenesis of kelp species, atlas of cell division and differentiation of Saccharina latissima
Authors: Theodorou Ioannis , Charrier Bénédicte 
Published: October 2023

ABSTRACT
In most organisms, 3D growth takes place at the onset of embryogenesis. In some brown algae, 3D growth occurs later in development, when the organism consists of several hundred cells. We studied the cellular events that take place when 3D growth is established in the embryo of the brown alga Saccharina, a kelp species. Semi-thin sections, taken from where growth shifts from 2D to 3D, show that 3D growth first initiates from symmetrical cell division in the monolayered lamina, and then is enhanced through a series of asymmetrical cell divisions in a peripheral monolayer of cells called the meristoderm. Then, daughter cells rapidly differentiate into cortical and medullary cells, characterised by their position, size and shape. In essence, 3D growth in kelps is based on a series of differentiation steps that occur rapidly after the initiation of a bilayered lamina, followed by further growth of the established differentiated tissues. Our study depicts the cellular landscape necessary to study cell-fate programming in the context of a novel mode of 3D growth in an organism phylogenetically distant from plants and animals.
CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility
Authors: Shimada Keisuke , Ikawa Masahito 
Published: October 2023

ABSTRACT
Sperm flagellum plays a crucial role in male fertility. Here, we generated Ccdc183 knockout mice using the CRISPR/Cas9 system to reveal the protein function of the testis-specific protein CCDC183 in spermiogenesis. We demonstrated that the absence of CCDC183 causes male infertility with morphological and motility defects in spermatozoa. Owing to the lack of CCDC183, centrioles after elongation of axonemal microtubules do not connect the cell surface and nucleus during spermiogenesis, which causes subsequent loss of cytoplasmic invagination around the flagellum. As a result, the flagellar compartment does not form properly and cytosol-exposed axonemal microtubules collapse during spermiogenesis. In addition, ectopic localization of accessory structures, such as the fibrous sheath and outer dense fibers, and abnormal head shape as a result of abnormal sculpting by the manchette are observed in Ccdc183 knockout spermatids. Our results indicate that CCDC183 plays an essential role in cytoplasmic invagination around the flagellum to form functional spermatozoa during spermiogenesis.
Sclerotome-derived PDGF signaling functions as a niche cue responsible for primitive erythropoiesis
Authors: Mao Aihua , Li Zhuyun , Ning Guozhu , Zhou Zhengrong , Wei Chiju , Li Jianchao , He Xinyu , Wang Qiang 
Published: October 2023

ABSTRACT
Primitive erythropoiesis serves a vital role in embryonic development, generating primitive red blood cells responsible for transportation of oxygen throughout the body. Although diverse niche factors are known to function in definitive hematopoiesis, the microenvironment contributing to primitive hematopoiesis remains largely elusive. Here, we report that platelet-derived growth factor (PDGF) signaling is required for erythroid progenitor differentiation in zebrafish. Ablating pdgfαa (also known as pdgfaa) and pdgfαb (also known as pdgfab) or blocking PDGF signaling with an inhibitor impairs erythroid progenitor differentiation, thus resulting in a significant decrease in the number of erythrocytes. We reveal that pdgfαb is expressed in sclerotomal cells, and that its receptor genes, pdgfra and pdgfrb, are expressed in the adjacent erythroid progenitor cells. Sclerotome-specific overexpression of pdgfαb effectively restores primitive erythropoiesis in pdgfαa−/−;pdgfαb−/− mutant embryos. In addition, we have defined ERK1/2 signaling as a downstream pathway of PDGF signaling during embryonic erythropoiesis. Taken together, our findings indicate that PDGF signaling derived from sclerotome functions as a niche cue for primitive erythropoiesis.
The katanin A-subunits KATNA1 and KATNAL1 act co-operatively in mammalian meiosis and spermiogenesis to achieve male fertility
Authors: Dunleavy Jessica E. M. , Graffeo Maddison , Wozniak Kathryn , O'Connor Anne E. , Merriner D. Jo , Nguyen Joseph , Schittenhelm Ralf B. , Houston Brendan J. , O'Bryan Moira K. 
Published: October 2023

ABSTRACT
Katanins, a class of microtubule-severing enzymes, are potent M-phase regulators in oocytes and somatic cells. How the complex and evolutionarily crucial, male mammalian meiotic spindle is sculpted remains unknown. Here, using multiple single and double gene knockout mice, we reveal that the canonical katanin A-subunit KATNA1 and its close paralogue KATNAL1 together execute multiple aspects of meiosis. We show KATNA1 and KATNAL1 collectively regulate the male meiotic spindle, cytokinesis and midbody abscission, in addition to diverse spermatid remodelling events, including Golgi organisation, and acrosome and manchette formation. We also define KATNAL1-specific roles in sperm flagellum development, manchette regulation and sperm-epithelial disengagement. Finally, using proteomic approaches, we define the KATNA1, KATNAL1 and KATNB1 mammalian testis interactome, which includes a network of cytoskeletal and vesicle trafficking proteins. Collectively, we reveal that the presence of multiple katanin A-subunit paralogs in mammalian spermatogenesis allows for ‘customised cutting’ via neofunctionalisation and protective buffering via gene redundancy.
Metabolic switches during development and regeneration
Authors: Mahmoud Ahmed I. 
Published: October 2023

ABSTRACT
Metabolic switches are a crucial hallmark of cellular development and regeneration. In response to changes in their environment or physiological state, cells undergo coordinated metabolic switching that is necessary to execute biosynthetic demands of growth and repair. In this Review, we discuss how metabolic switches represent an evolutionarily conserved mechanism that orchestrates tissue development and regeneration, allowing cells to adapt rapidly to changing conditions during development and postnatally. We further explore the dynamic interplay between metabolism and how it is not only an output, but also a driver of cellular functions, such as cell proliferation and maturation. Finally, we underscore the epigenetic and cellular mechanisms by which metabolic switches mediate biosynthetic needs during development and regeneration, and how understanding these mechanisms is important for advancing our knowledge of tissue development and devising new strategies to promote tissue regeneration.
Sex-Specific Impact of CARM1 in Skeletal Muscle Adaptations to Exercise
Authors: vanLieshout Tiffany L. , Stouth Derek W. , Raziee Rozhin , Sraka Anne-Sophie J. , Masood Hooriya A. , Ng Sean Y. , Mattina Stephanie R. , Mikhail Andrew I. , Manta Alexander , Ljubicic Vladimir 
Published: October 2023

ABSTRACT

Purpose
The purpose of this study was to determine how the intersection of coactivator-associated arginine methyltransferase 1 (CARM1) and biological sex impacts skeletal muscle adaptations to chronic physical activity.

Methods
12-week-old female (F) and male (M) wild-type (WT) and CARM1 skeletal muscle-specific knockout mice (mKO) were randomly assigned to sedentary (SED) or voluntary wheel running (VWR) experimental groups. For 8 weeks, the animals in the VWR cohort had volitional access to running wheels. Subsequently, we performed whole-body functional tests, and 48 hours later muscles were harvested for molecular analysis. Western blotting, enzyme activity assays, as well as confocal and transmission electron microscopy (TEM) were used to examine skeletal muscle biology.

Results
Our data reveal a sex-dependent reduction in VWR volume caused by muscle-specific ablation of CARM1, as F CARM1 mKO mice performed less chronic, volitional exercise than their WT counterparts. Regardless of VWR output, exercise-induced adaptations in physiological function were similar between experimental groups. A broad panel of protein arginine methyltransferase (PRMT) biology measurements, including markers of arginine methyltransferase expression and activity, were unaffected by VWR, except for CARM1 and PRMT7 protein levels, which decreased and increased with VWR, respectively. Changes in myofiber morphology and mitochondrial protein content showed similar trends among animals. However, a closer examination of TEM images revealed contrasting responses to VWR in CARM1 mKO mice compared to WT littermates, particularly in mitochondrial size and fractional area.

Conclusions
The present findings demonstrate that CARM1 mKO reduces daily running volume in F mice, as well as exercise-evoked skeletal muscle mitochondrial plasticity, which indicates that this enzyme plays an essential role in sex-dependent differences in exercise performance and mitochondrial health.
Analysis of carbon nano particle variant as the propellant fuel to increase specific impulses of rockets
Authors: Navalino RDA , Muda NRS , Hafizah MAE , Ruyat Y 
Published: October 2023

Background: This study compares propellant fuels' specific thrust and impulse parameters using nanocarbon variant fuels from coconut shells and coal. Specific impulses and impulses are essential parameters that determine rocket performance. The specific thrust and impulses are influenced by fuel type, material composition, heat flow, and burning time parameters. The characteristics of nanocarbon as a fuel are proven to have high-value features and a long combustion time. This parameter is essential to add value to specific thrusts and impulses. Methods: The method to prove the quality of solid fuel material was done experimentally. The composition of the propellant fuel variant mixed with NH4ClO4 as an oxidizer through the printing process and sample testing was carried out using scanning electron microscope (SEM), X-ray diffraction analysis (XRD), combustion time, and specific impulse tests. Results: Test results with the composition of CBK2 (Coconut Shell + NH4ClO4 + Binder + Al = 15: 70: 10: 5) produced a specific impulse of 267 seconds or an increase of 37 seconds without carbon. CBB2 (Coal + NH4ClO4 + Binder + Al = 15: 70: 10: 5) produced a specific impulse of 261 seconds or an increase of 31 seconds. Conclusions: The composition of nanocarbon as a solid fuel propellant has been proven to increase a rocket's thrust and specific impulses. After all the material variants have been tested for thrust and specific impulse, the best rocket propellant is CBK2.
Patient satisfaction of specialist nurse-led renal cancer follow-up
Authors: Vanalia Parthvi , Paracha Areej , Herdman Jennifer , Zelhof Bachar 
Published: October 2023

Background: For many patients with locally advanced renal cancer, management is surgery followed by surveillance imaging for several years. There is no concrete data regarding specialist nurse follow-up for patients after they have had surgery. Aims: The authors' aim was to assess patient satisfaction with specialist nurse follow-up for patients with renal cancer who have undergone surgery. Methods: A questionnaire was used to measure patient satisfaction of the specialist nurse consultation. There were 100 patients invited who had been followed up in the nurse-led clinic between January 2020 and May 2021. These patients were given a phone call to complete the questionnaire. Findings: The audit revealed that in terms of general satisfaction, 97.1% of patients felt totally satisfied with their visit/consultation at the specialist nurse clinic. Furthermore, 94.2% of patients felt that they had good continuity of care with specialist nurse follow-up. Conclusion: Nurse-led follow-up was found to have high levels of patient satisfaction.
Effectiveness of a telenursing intervention program in reducing exacerbations in patients with chronic respiratory failure receiving noninvasive positive pressure ventilation: A randomized controlled trial
Authors: Shimoyama Makoto , Yoshida Shiori , Takahashi Chikako , Inoue Mizue , Sato Naoko , Sato Fumiko 
Published: October 2023

Telenursing for patients with chronic respiratory failure receiving noninvasive positive pressure ventilation (NPPV) is an important aid in reducing exacerbations; however, there is insufficient evidence. This randomized controlled trial investigated the effectiveness of a telenursing intervention program in reducing exacerbations in patients with chronic respiratory failure receiving NPPV at home. We included patients receiving NPPV at home who could handle a tablet device. The intervention group (n = 15) underwent an information and communications technology-based telenursing intervention program in addition to usual care; the control group (n = 16) received the usual care only. The telenursing intervention program comprised telemonitoring and health counseling sessions via videophone. The intervention was evaluated once at enrollment and after 3 months. The primary endpoints were the number of unscheduled outpatient visits, hospitalizations, and hospital days. The secondary endpoints included the St. George’s Respiratory Questionnaire (SGRQ) score, Euro QOL 5 Dimension score, Self-Care Agency Questionnaire (SCAQ) score, pulmonary function tests, and 6-min walking distance. We used the Mann–Whitney U test for our analysis. We found no significant differences between the intervention and control groups at enrollment. Then, the differences between the endpoints at baseline and 3 months after enrollment were calculated and used to compare both groups. At follow-up, the number of routine outpatient visits for acute exacerbations (p = .045), the number of hospitalizations (p = .037), the number of hospital days (p = .031), SGRQ (p = .039) score, and SCAQ (p = .034) score were significantly different. The increase in the number of unscheduled outpatient visits in the intervention group during follow-up was attributed to acute exacerbations and a significant decrease in the number of hospitalizations and hospital days. Hence, the telenursing intervention program may be effective in reducing exacerbations in patients with chronic respiratory failure receiving NPPV at home.
Trial registration: UMIN Clinical Trials Registry (UMIN-CTR) UMIN000027657.
Development and effects of salutogenesis program for adolescents with moyamoya disease: A randomized controlled trial
Authors: Yeom Insun , Oh Won-oak 
Published: October 2023

Background
Disease-specific interventions for management and health behavior implementation are needed to improve the health and quality of life of adolescents with moyamoya disease.

Objective
This study aimed to develop a program for adolescents with moyamoya disease based on the salutogenesis theory, which focuses on the process of enhancing health through successful adaptation to external stressors, and to evaluate its effectiveness.

Methods
A randomized controlled trial was performed according to the CONSORT guidelines. This preliminary research and experimental treatment were conducted at a Severance Hospital ward and outpatient clinic among 48 participants randomized into the intervention (seven sessions of salutogenesis program, n = 24) or the control group (one session of one-to-one moyamoya disease education program, n = 24) from September 6, 2018 to January 4, 2019. Changes in the following study outcomes were reported: “knowledge of moyamoya disease,” “social support,” “sense of coherence,” “moyamoya disease health behavior,” “stress,” “depression,” “subjective health status,” “frequency of ischemic symptoms,” and “quality of life”.

Results
The salutogenesis program improved the knowledge and social support of adolescents with illness-related problems and helped them attain healthy behaviors and stress reduction. It was confirmed to be effective in improving their quality of life.

Conclusions
The salutogenesis program for adolescents with moyamoya disease effectively improved the generalized resistance resources and sense of coherence in adolescents with moyamoya disease.

Trial registration
Korean Clinical Research Information Service registry, KCT0006869.
Google Search Trends to assess public interest in and concern about Vuity for treating presbyopia
Authors: Wakabayashi Taku , Mansour Hana A. , Abishek Robert M. , Sridhar Jayanth , Cohen Michael N. , Xu David , Deaner Jordan , Yonekawa Yoshihiro , Hsu Jason , Kuriyan Ajay E. 
Published: October 2023

Purpose
To assess public awareness, interest, and concerns regarding Vuity (1.25% pilocarpine hydrochloride ophthalmic solution), an eye drop for the treatment of presbyopia, based on Google Trends.

Methods
We used Google Trends that provides a relative search volume for queried terms, to evaluate searches for Vuity from June 30, 2021, to June 30, 2022, in the United States. The data for this study were downloaded on June 30, 2022. Main outcome measures were changes in relative search volumes for the terms “Vuity,” “Eye drops for reading,” “Eye drops for near vision,” “Presbyopia,” “Pilocarpine,” and related popular search terms, such as “Vuity side effects,” and “Vuity retinal detachment”.

Results
Since the approval of Vuity on October 29, 2021, notable increases in the relative search volumes occurred for Vuity in October 2021, December 2021, and from March to April 2022, which coincided with its approval, availability, and subsequent direct-to-consumer advertising based on positive results of clinical trials. The direct-to-consumer advertising had the greatest impact on the search volume for Vuity. Specific interests included Vuity cost, where to buy it, and its side effects. Retinal detachment was the most highly searched Vuity side effect. Geographic variation was evident, with the relative search volumes highest for “Vuity” in Wyoming, followed by North Dakota.

Conclusion
Google Trends is a useful tool for monitoring increases in public interest in Vuity for presbyopia. Public concerns regarding side effects warrant further real-world investigations of the causal relationship between Vuity and retinal detachment.