bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of transcranial direct current stimulation paired with neuromuscular electrical stimulation on swallowing function in post stroke dysphagia
Authors: El Nahas Nevine , Shokri Hossam , Refaat Ahmed , Mousa Hanaa , Hamid Aya , Abdel Monem Amr , AbuShady Eman 
Published: February 2024

Abstract
Background
Transcranial direct current stimulation (tDCS) and neuromuscular electrical stimulation (NMES) are noninvasive neuromodulation techniques that have shown positive effects in a variety of neurological disorders. Most protocols apply one modality at a time. Here we tested the effect of tDCS applied together with NMES on patients with dysphagia after acute stroke. To assess the efficacy of combined tDCS and NMES on improvement of dysphagia after acute stroke, guided by Fiber-optic endoscopic evaluation of swallowing (FEES). This study is a double-blinded randomized case-controlled study conducted in a University hospital. This study enrolled 48 patients diagnosed by FEES and assigned to 3 groups of 16 patients each. The first group received both tDCS and NMES, the second group received NMES only and the third group received sham NMES. Gugging Swallowing Screen (GUSS) test was done before and after intervention.

Results
Significant improvement was seen in all tested materials on GUSS test in tDCS/NMES group. While in the other two groups, there was only improvement for safety of liquid swallowing.

Conclusion
This study shows that the combined application of tDCS and NMES has an advantage in improvement of PSD over active NMES and sham NMES groups in all materials tested by GUSS.
Emerging advanced approaches for diagnosis and inhibition of liver fibrogenesis
Authors: Addissouky Tamer A. , Ali Majeed M. A. , Sayed Ibrahim El Tantawy El , Wang Yuliang 
Published: February 2024

Abstract
Background
Liver fibrosis results from chronic liver injury and is characterized by excessive deposition of extracellular matrix proteins including collagen. It can progress to cirrhosis and liver failure.

Main body of the abstract
Multiple cellular signaling pathways drive hepatic stellate cell activation and fibrogenesis. Advances in biomarkers, imaging modalities, and omics platforms enable noninvasive diagnosis and staging of liver fibrosis. Emerging antifibrotic approaches include medications like pirfenidone, obeticholic acid, and monoclonal antibodies targeting pro-fibrotic mediators. Cell therapies using mesenchymal stem cells demonstrate antifibrotic potential through paracrine immunosuppression. Tissue-engineered liver grafts and biomaterial carriers for localized drug delivery are promising technologies. Microfluidic liver-on-a-chip platforms with patient-derived cells provide unprecedented models to study human liver fibrosis and test drug candidates.

Short conclusion
Significant progress has elucidated mechanisms underlying liver fibrogenesis and uncovered novel therapeutic targets. Ongoing challenges include translating preclinical findings, improving antifibrotic efficacy, and enabling personalized precision medicine approaches. Further research into combinatorial therapies, biomarkers, and tissue engineering technologies will advance the treatment of liver fibrosis from all causes.
Value for money: a cost-effectiveness analysis of microplastic analytics in seawater
Authors: Meyers Nelle , Kopke Kathrin , Buhhalko Natalja , Mattsson Karin , Janssen Colin R. , Everaert Gert , De Witte Bavo 
Published: February 2024

AbstractThe availability of many microplastic analysis methods is challenging for researchers and policy makers when tasked with choosing optimal methods for their research question and a given budget. In this study, a cost-effectiveness analysis of methods for microplastic analysis in seawater was performed using survey data acquired from experts. Total analysis cost per method was determined accounting for labour and equipment costs, while method effectiveness was scored based on their ability to confirm the plastic nature of particles, their minimum detectable particle size, and other parameters. Results were validated and discussed during two workshops with scientists and policy makers. The resulting predictive tools allow to identify the most cost-effective methods for specific scenarios, and their associated cost. They mark an important step towards a more effective and informed approach to monitoring and managing microplastic pollution in the marine environment, ultimately contributing to the protection of marine ecosystems and human health.
Bearing capacity prediction of the concrete pile using tunned ANFIS system
Authors: Gu Wei , Liao Jifei , Cheng Siyuan 
Published: February 2024

AbstractThe design process for pile foundations necessitates meticulous deliberation of the calculation pertaining to the bearing capacity of the piles. The primary objective of this work was to investigate the potential use of Coot bird optimization ($${\text{CBO}}$$
CBO
) techniques in predicting the load-bearing capacity of concrete-driven piles. Despite the availability of several suggested models, the investigation of Coot bird optimization ($${\text{CBO}}$$
CBO
) for estimating the pile-carrying capacity has been somewhat neglected in this research. This work presents and validates a unique approach that combines the Coot bird optimization ($${\text{CBO}}$$
CBO
) model with the Multi-layered perceptron ($${\text{MLP}}$$
MLP
) neural network and adaptive neuro-fuzzy inference system ($${\text{ANFIS}}$$
ANFIS
). The findings of 472 different driven pile static load tests were put in a database. The proposed framework's building, validation, and testing stages were each accomplished utilizing the training set (70%), validation set (15%), and testing set (15%) of the dataset, respectively. According to the findings, $${{\text{MLP}}}_{{\text{CBO}}}$$

MLP
CBO

and $${{\text{ANFIS}}}_{{\text{CBO}}}$$

ANFIS
CBO

both offer remarkable possibilities for accurately predicting the pile-bearing capacity of a given structure. The $${R}^{2}$$

R

2

values for $${{\text{ANFIS}}}_{{\text{CBO}}}$$

ANFIS
CBO

during the training stage were 0.9874, while during the validating stage, they were 0.9785, and during the testing stage they were 0.987. After considering various kinds of performance studies and contrasting them with existing literature, it has been concluded that the $${{\text{ANFIS}}}_{{\text{CBO}}}$$

ANFIS
CBO

model provides a more appropriate calculation of the bearing capacity of concrete-driven piles.
Asymmetric acoustic splitting with the dual-layers of binary metagratings
Authors: Liu Zhengang , Chang Shuai , Xue Shibei , Ju Fangfang , Qian Shengyou 
Published: February 2024

Abstract
Here, we exhibit the asymmetric acoustic splitting with the dual-layers of binary metagratings (BMs), i.e., BM1 and BM2. For the positive incidence (PI) normally from the BM1 side, acoustic waves are freely transmitted through the BM1 and split into two beams with the transmitted angles at

and

, then the two beams are freely transmitted through the BM2 with the transmitted angles at

and

. For the negative incidence (NI) normally from the BM2 side, acoustic waves are wholly reflected. Hence, the asymmetric acoustic splitting is observed. Here, the dual-layers of BMs are exemplified by the coating cells with different period lengths. Excellent agreement can be observed between the numerical simulations and the theoretical analysis. Our proposal may be applied in noise control and acoustic communication.
Anomalous metallic conductivity and short-range ferromagnetic correlation in high-pressure synthesized pyrochlore
Authors: Zhao Jianfa , Deng Zheng , Zhang Jun , Peng Yi , Shi Luchuan , Min Baosen , Duan Lei , Li Wenmin , Cao Lipeng , Chen Jeng-Lung , Hu Zhiwei , Yu Runze , Jin Changqing 
Published: February 2024

Abstract
Iridates show fascinating properties due to the unpredictable ground states of their Ir cations. Generally, Ir5+(5d
4) systems exhibit insulating nonmagnetic states owing to the strong spin-orbit coupling (SOC). Herein a new pyrochlore iridate Hg2Ir2O7 with an Ir5+ charge state synthesized by high-pressure technique is reported. Hg2Ir2O7 crystallizes in the typical cubic pyrochlore crystal structure. The Ir5+ valence state is evidenced by the XAS spectrum. Surprisingly, Hg2Ir2O7 displays short-range ferromagnetic correlations at low temperatures as evidenced by S-shape field-dependent magnetization curves, positive magnetoresistance, and magnetic excitations in specific heat. Furthermore, it also shows metallic conduction and large electron component of specific heat. These results all indicate that Ir5+ in Hg2Ir2O7 deviates from SOC-dominated insulating nonmagnetic states.
Compliance With Federal Price Transparency Rules and Cost Estimation at United States Hospitals With Neurosurgical Training Programs
Authors: Giantini-Larsen Alexandra , Pandey Abhinav , Abou-Mrad Zaki , Tata Nalini , Barzilai Ori , Bilsky Mark , Newman W. Christopher 
Published: February 2024

BACKGROUND AND OBJECTIVES:
The Centers for Medicare & Medicaid Services implemented federal requirements on January 1, 2021, under the Public Health Service Act that require hospitals to provide a list of payer-negotiated prices or “standard charges” in a machine-readable file and in a patient-friendly online estimator for standard services. We sought to assess compliance by United States hospitals associated with neurosurgical training programs with these federal requirements for 11 common neurosurgical procedures.

METHODS:
We performed a cross-sectional analysis in March 2023 of 116 United States hospitals associated with a neurosurgical training program to assess compliance with the new federal requirements to have a machine-readable, downloadable file with standard charges and a patient-friendly online estimator for two spinal procedures.

RESULTS:
A total of 110/114 (96.5%) hospitals were compliant with the requirement for a machine-readable file with payer-negotiated prices. A total of 47/110 hospitals (42.7%) were compliant with downloadable machine-readable files and reported at least one payer-negotiated price for 1 of the 11 common neurosurgical procedures. A total of 45/110 (40.9%) used bundled Diagnosis-Related Group codes, and 18/110 (16.4%) did not contain any price information for neurosurgical procedures. For neurosurgical procedures, the percent difference between the average negotiated private insurance and Medicare price per procedure ranged from 17.5% to 77.6%. Medicare and private insurance data for each procedure were available on average for 10.3 states (SD = 3.8) and 15.6 states (SD = 4.8), respectively.

CONCLUSION:
While hospital compliance with federal requirements for machine-readable files with payer-negotiated prices was high, availability of payer-negotiated prices for 4 major insurance types across 11 common neurosurgical procedures based on Current Procedural Terminology codes was sparce. Consequently, meaningful conclusions on procedure-related charges for elective procedures are difficult for patients to make because of the unintelligible format of data and a lack of reporting of charges per Current Procedural Terminology code in a comprehensive manner.
Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo
Authors: Athilingam Thamarailingam , Nelanuthala Ashwin V. S. , Breen Catriona , Karedla Narain , Fritzsche Marco , Wohland Thorsten , Saunders Timothy E. 
Published: February 2024

ABSTRACT
Morphogen gradients provide essential positional information to gene networks through their spatially heterogeneous distribution, yet how they form is still hotly contested, with multiple models proposed for different systems. Here, we focus on the transcription factor Bicoid (Bcd), a morphogen that forms an exponential gradient across the anterior-posterior (AP) axis of the early Drosophila embryo. Using fluorescence correlation spectroscopy we find there are spatial differences in Bcd diffusivity along the AP axis, with Bcd diffusing more rapidly in the posterior. We establish that such spatially varying differences in Bcd dynamics are sufficient to explain how Bcd can have a steep exponential gradient in the anterior half of the embryo and yet still have an observable fraction of Bcd near the posterior pole. In the nucleus, we demonstrate that Bcd dynamics are impacted by binding to DNA. Addition of the Bcd homeodomain to eGFP::NLS qualitatively replicates the Bcd concentration profile, suggesting this domain regulates Bcd dynamics. Our results reveal how a long-range gradient can form while retaining a steep profile through much of its range.
Sall4 regulates posterior trunk mesoderm development by promoting mesodermal gene expression and repressing neural genes in the mesoderm
Authors: Pappas Matthew P. , Kawakami Hiroko , Corcoran Dylan , Chen Katherine Q. , Scott Earl Parker , Wong Julia , Gearhart Micah D. , Nishinakamura Ryuichi , Nakagawa Yasushi , Kawakami Yasuhiko 
Published: February 2024

ABSTRACT
The trunk axial skeleton develops from paraxial mesoderm cells. Our recent study demonstrated that conditional knockout of the stem cell factor Sall4 in mice by TCre caused tail truncation and a disorganized axial skeleton posterior to the lumbar level. Based on this phenotype, we hypothesized that, in addition to the previously reported role of Sall4 in neuromesodermal progenitors, Sall4 is involved in the development of the paraxial mesoderm tissue. Analysis of gene expression and SALL4 binding suggests that Sall4 directly or indirectly regulates genes involved in presomitic mesoderm differentiation, somite formation and somite differentiation. Furthermore, ATAC-seq in TCre; Sall4 mutant posterior trunk mesoderm shows that Sall4 knockout reduces chromatin accessibility. We found that Sall4-dependent open chromatin status drives activation and repression of WNT signaling activators and repressors, respectively, to promote WNT signaling. Moreover, footprinting analysis of ATAC-seq data suggests that Sall4-dependent chromatin accessibility facilitates CTCF binding, which contributes to the repression of neural genes within the mesoderm. This study unveils multiple mechanisms by which Sall4 regulates paraxial mesoderm development by directing activation of mesodermal genes and repression of neural genes.
TIAM-1 regulates polarized protrusions during dorsal intercalation in the Caenorhabditis elegans embryo through both its GEF and N-terminal domains
Authors: Zhu Yuyun , Tesone Zoe , Tan Minyi , Hardin Jeff 
Published: February 2024

ABSTRACT
Mediolateral cell intercalation is a morphogenetic strategy used throughout animal development to reshape tissues. Dorsal intercalation in the Caenorhabditis elegans embryo involves the mediolateral intercalation of two rows of dorsal epidermal cells to create a single row that straddles the dorsal midline, and thus is a simple model to study cell intercalation. Polarized protrusive activity during dorsal intercalation requires the C. elegans Rac and RhoG orthologs CED-10 and MIG-2, but how these GTPases are regulated during intercalation has not been thoroughly investigated. In this study, we characterized the role of the Rac-specific guanine nucleotide exchange factor (GEF) TIAM-1 in regulating actin-based protrusive dynamics during dorsal intercalation. We found that TIAM-1 can promote formation of the main medial lamellipodial protrusion extended by intercalating cells through its canonical GEF function, whereas its N-terminal domains function to negatively regulate the generation of ectopic filiform protrusions around the periphery of intercalating cells. We also show that the guidance receptor UNC-5 inhibits these ectopic filiform protrusions in dorsal epidermal cells and that this effect is in part mediated via TIAM-1. These results expand the network of proteins that regulate basolateral protrusive activity during directed rearrangement of epithelial cells in animal embryos.
Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling
Authors: Nguyen Jo , Wang Lu , Lei Wen , Hu Yechen , Gulati Nitya , Chavez-Madero Carolina , Ahn Henry , Ginsberg Howard J. , Krawetz Roman , Brandt Matthias , Betz Timo , Gilbert Penney M. 
Published: February 2024

ABSTRACT
Understanding how biophysical and biochemical microenvironmental cues together influence the regenerative activities of muscle stem cells and their progeny is crucial in strategizing remedies for pathological dysregulation of these cues in aging and disease. In this study, we investigated the cell-level influences of extracellular matrix (ECM) ligands and culture substrate stiffness on primary human myoblast contractility and proliferation within 16 h of plating and found that tethered fibronectin led to stronger stiffness-dependent responses compared to laminin and collagen. A proteome-wide analysis further uncovered cell metabolism, cytoskeletal and nuclear component regulation distinctions between cells cultured on soft and stiff substrates. Interestingly, we found that softer substrates increased the incidence of myoblasts with a wrinkled nucleus, and that the extent of wrinkling could predict Ki67 (also known as MKI67) expression. Nuclear wrinkling and Ki67 expression could be controlled by pharmacological manipulation of cellular contractility, offering a potential cellular mechanism. These results provide new insights into the regulation of human myoblast stiffness-dependent contractility response by ECM ligands and highlight a link between myoblast contractility and proliferation.
Palmitoylation of proteolipid protein M6 promotes tricellular junction assembly in epithelia of Drosophila
Authors: Schleutker Raphael , Luschnig Stefan 
Published: February 2024

ABSTRACT
Tricellular junctions (TCJs) seal epithelial cell vertices and are essential for tissue integrity and physiology, but how TCJs are assembled and maintained is poorly understood. In Drosophila, the transmembrane proteins Anakonda (Aka, also known as Bark), Gliotactin (Gli) and M6 organize occluding TCJs. Aka and M6 localize in an interdependent manner to vertices and act jointly to localize Gli, but how these proteins interact to assemble TCJs was not previously known. Here, we show that the proteolipid protein M6 physically interacts with Aka and with itself, and that M6 is palmitoylated on conserved juxta-membrane cysteine residues. This modification promotes vertex localization of M6 and binding to Aka, but not to itself, and becomes essential when TCJ protein levels are reduced. Abolishing M6 palmitoylation leads to delayed localization of M6 and Aka but does not affect the rate of TCJ growth or mobility of M6 or Aka. Our findings suggest that palmitoylation-dependent recruitment of Aka by M6 promotes initiation of TCJ assembly, whereas subsequent TCJ growth relies on different mechanisms that are independent of M6 palmitoylation.
2′-O-ribose methylation levels of ribosomal RNA distinguish different types of growth arrest in human dermal fibroblasts
Authors: Yang Guohuan , Schmid-Siegel Maximilian , Heissenberger Clemens , Kos-Braun Isabelle C. , Prechtl Martina , Meca-Laguna Gabriel , Rocha Marta , Wagner-Schrittwieser Anja , Pils Vera , Meixner Barbara , Tav Koray , Hengstschläger Markus , Grillari Johannes , Koš Martin , Schosserer Markus 
Published: February 2024

ABSTRACT
The 2′-O-methylation (2′-O-Me) of ribosomal RNA (rRNA) shows plasticity that is potentially associated with cell phenotypes. We used RiboMeth-seq profiling to reveal growth arrest-specific 2′-O-Me patterns in primary human dermal fibroblasts from three different donors. We exposed cells to hydrogen peroxide to induce cellular senescence and to high cell densities to promote quiescence by contact inhibition. We compared both modes of cell cycle arrest to proliferating cells and could indeed distinguish these conditions by their overall 2′-O-Me patterns. Methylation levels at a small fraction of sites showed plasticity and correlated with the expression of specific small nucleolar RNAs (snoRNAs) but not with expression of fibrillarin. Moreover, we observed subtle senescence-associated alterations in ribosome biogenesis. Knockdown of the snoRNA SNORD87, which acts as a guide for modification of a hypermethylated position in non-proliferating cells, was sufficient to boost cell proliferation. Conversely, depletion of SNORD88A, SNORD88B and SNORD88C, which act as guides for modification of a hypomethylated site, caused decreased proliferation without affecting global protein synthesis or apoptosis. Taken together, our findings provide evidence that rRNA modifications can be used to distinguish and potentially influence specific growth phenotypes of primary cells.
FEATURES OF PROFESSIONALISM OF CORRECTIONAL INSTITUTION EMPLOYEES WITH DIFFERENT LEVELS OF MOTIVATION FOR PHYSICAL AND SPORTS ACTIVITIES
Authors: Kuklina Larisa V. 
Published: February 2024

Professional development plays a key role in the career and personal growth of every specialist. It includes not only the acquisition of new knowledge and skills, but also the formation of professional self-awareness, which allows the employee to realize his importance and responsibility to society.
Therefore, the problem arises of searching for factors that influence the professionalism of employees at different stages of professional activity and developing a model of psychological and acmeological support that contributes to the formation of their professional competence.
Purpose of the work: to study the features of motivation for physical culture and sports activities of employees of the penal system and its relationship with professional competence
Materials and methods of research. The study was conducted on the basis of the institutions of the Federal Penitentiary Service of Russia in the Vologda region, 2 groups of respondents took part in it: group 1 – 25 employees of a special forces detachment involved in sports (athletes with ranks in wrestling) aged 23 to 27 years; group 2 – 25 correctional colony employees aged from 23 to 27 years old who are not involved in sports (employees of the educational department, administration employees).
The methods “Motives for Playing Sports” by A.V. Shaboltas, expert assessment of the professional competence of employees of the penal system using an adapted “methodology for a comprehensive assessment of the professional performance of a state civil servant.”
Research results and discussion. The results of the study showed that employees of the penitentiary system with different levels of motivation for physical education and sports activities have differences in professional competence. There is a relationship between professional competence and motivation for physical education and sports activities among correctional officers.
The practical significance of the study lies in the possibility of applying the results in the development of programs for the development of professional competence of correctional officers, designed on the basis of differences in their motivation for physical education and sports activities
The conducted research allows us to formulate the following recommendations:
Purposeful formation of motivation for physical culture and sports activities of employees of the penal system through the development of internal attitudes and external stimulation will also contribute to the formation of health-preserving competence of employees.
Creating conditions for the formation of motivation for physical culture and sports activities of employees of the penal system will contribute not only to increasing the level of physical fitness as a component of the professional competence of employees, but will also stimulate the manifestation of their subjectivity.