bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sfrp1 as a Pivotal Paracrine Factor in the Trained Pericardial Stem Cells that Foster Reparative Activity
Authors: Zhu Hongtao , Liu Xueqing , Ouyang Weili , Hao Yingcai , Ding Zheheng , Tan Kezhe , Tang Jianfeng , Zhao Jianfeng , Ding Xiaojun , Teng Zenghui , Deng Xiaoming , Wu Weidong , Ding Zhaoping 
Published: November 2023

Abstract
Tissue damage often induces local inflammation that in turn dictates a series of subsequential responses, such as stem cell activation and growth, to maintain tissue homeostasis. The aim of the study is to testify the possibility of using inflammation-trained stem cells as optimal donor cells to augment the efficacy of cell therapy. The pericardial stem/stromal cells derived from the animals after myocardial infarction (MI-pSC) showed an enhanced myogenic potential and augmented reparative activity after transplantation in the injured hearts, as compared to the Sham-pSC. Bulk RNA-Seq analysis revealed significant upregulation of a panel of myogenic and trophic genes in the MI-pSC and, notably, Sfrp1 as an important anti-apoptotic factor induced robustly in the MI-pSC. Injection of the MI-pSC yielded measurable numbers of surviving cardiomyocytes (Tunel and Casp-3 negative) within the infarct area, but the effects were significantly diminished by siRNA-based silence of Sfrp1 gene in the pSC. Primed Sham-pSC with pericardial fluid from MI rats mimicked the upregulation of Sfrp1 and enhanced myogenic potential and reparative activity of pSC. Taken together, our results illustrated the inflammation-trained pSC favor a reparative activity through upregulation of Sfrp1 gene that confers anti-apoptotic activity in the injured cardiomyocytes. Therefore, the active form of stem cells may be used as a cardiac protective agent to boost therapeutical potential of stem cells.
Phylogeny, taxonomy, and biogeography of the Sphaeriinae (Bivalvia: Sphaeriidae)
Authors: Bespalaya Yulia V , Vinarski Maxim V , Aksenova Olga V , Babushkin Evgeniy S , Gofarov Mikhail Yu , Kondakov Alexander V , Konopleva Ekaterina S , Kropotin Alexander V , Mabrouki Youness , Ovchankova Nadezda B , Palatov Dmitry M , Sokolova Svetlana E , Shevchenko Alexander R , Travina Oksana V , Taybi Abdelkhaleq F , Soboleva Alena A , Zubrii Natalia A , Bolotov Ivan N 
Published: November 2023

Abstract
The subfamily Sphaeriinae is a diverse and ecologically significant group of freshwater invertebrates, playing a keystone role in the energy flow and nutrient cycling in aquatic ecosystems. Despite a long history of its study, the taxonomic structure and species content of this group are a matter of ongoing disputes between malacologists. We clarify the taxonomy, distribution, and evolutionary biogeography of the Sphaeriinae around the world based on the largest dataset of DNA sequences of these clams ever accumulated, which includes the data on specimens collected from various continents and countries around the globe. Our 16S rRNA and two-locus phylogenies reveal that the Sphaeriinae is a monophyletic group, containing at least seven genus-level clades: Sphaerium Scopoli, 1777, Pisidium C. Pfeifffer, 1821 (s.s.), Euglesa Jenyns, 1832, Odhneripisidium Kuiper, 1962, Conventus Pirogov & Starobogatov stat. nov., 1974, ‘Pisidium’ sterkianum group, and Hindupisidium Vinarski & Bespalaya gen. nov. Our 16S rRNA phylogenetic tree contains 80 well-supported clades corresponding to the putative species-level taxa. Four species new to science are described here: Euglesa kolymensis Bespalaya, Aksenova & Bolotovsp. nov., Euglesa kyrgyzica Bespalaya, Aksenova, Kondakov & Palatovsp. nov., Euglesa moroccana Bespalaya, Vinarski, Aksenova, Mabrouki, Kondakov & Palatovsp. nov., Odhneripisidium caucasus Bespalaya, Vinarski, Aksenova, Kondakov & Palatovsp. nov. Our fossil-calibrated phylogeny and generalized biogeographic model indicate that the most recent common ancestor of the Sphaeriidae could have originated somewhere within a broad area covering China near the Lower Cretaceous boundary.
Underexplored diversity in Palaearctic Miltogramma Meigen, 1803 (Diptera: Sarcophagidae)
Authors: Szpila Krzysztof , Walczak Kinga , Grzywacz Andrzej , Soszyńska Agnieszka , Akbarzadeh Kamran , Bystrowski Cezary , Pape Thomas 
Published: November 2023

Abstract
Current knowledge of the global diversity of schizophoran flies, the megadiverse group that evolved during the episode of rapid dipteran radiations in the Early Cenozoic, is far from satisfactory. A significant part of this radiation is flesh flies (Sarcophagidae), which include about 3000 known species. Miltogramma Meigen, the largest genus of the flesh fly subfamily Miltogramminae, includes 119 known species with a peak of diversity in the Middle East and Central Asia. The taxonomy of the genus is acknowledged to be challenging, partly due to logistical issues associated with the deposition of type specimens in less accessible Russian institutions. Six new distinctive species of Miltogramma collected during recent expeditions to Iran and Uzbekistan are described using methods of integrative taxonomy: Miltogramma manouchehrii Szpila & Pape, sp. nov., M. mikolajczyki Szpila & Pape, sp. nov., M. rognesi Szpila & Pape, sp. nov., M. soszynskii Szpila & Pape, sp. nov., M. thompsoni Szpila & Pape, sp. nov., and M. vervesi Szpila & Pape, sp. nov. Flies are characterized by secondary sexual dimorphism expressed in the form of species-specific chaetotaxy of the male fore tarsus, a character related to courtship behaviour. The reconnaissance surveys to both of the mentioned countries performed in the last decade brought information about a large number of potential new species of Miltogramma, despite the taxonomic work performed in this area in the past. Describing the majority of them requires extensive revisionary work, which involves accessing the type specimens and applying modern methods of species delimitation.
Safety and Efficacy Outcomes Following Spinal Endoscopic Procedures for Thoracic Ligamentous Ossification
Authors: Ye Jingyao , Guo Wenlong , Hu Youpeng , Fan Xiaohong 
Published: November 2023

Study Design.
Systematic review and meta-analysis.

Objective.
This study systematically reviewed and evaluated the safety and efficacy of spinal endoscopic techniques as a treatment for thoracic ligamentum flavum ossification (TOLF).

Summary of Background Data.
The use of spinal endoscopic techniques for the treatment of TOLF has increased in recent years. The present study is the first comprehensive systematic review and meta-analysis focused on the use of spinal endoscopic techniques for TOLF.

Methods.
The Cochrane Central, PubMed, Web of Science, and Embase databases were systematically searched for studies focused on patients undergoing spinal endoscopic techniques to treat symptomatic TOLF.

Results.
This meta-analysis included 23 studies. We included 323 patients (177 males, 146 females) with a mean age of 58.40±10.06 years, with 304 total recorded lesion locations of which 245 were located in the lower thoracic spine. Complications affected 35/323 patients, and the mean operative duration for 305 patients was 108.15±47.34 minutes. For 187 patients, the mean operative bleeding was 25.13±12.54 mL, while for 87 patients the mean duration of hospitalization was 4.59±1.93 days. At last follow-up，functional assessment was performed for 260 patients, of whom 200 were in excellent condition, visual analog scale (VAS) scores were assessed for 160 patients, with a mean improvement of 4.40 (3.95,4.86) Japanese Orthopedic Association (JOA) scores were recorded for 115 patients, with a mean improvement of 2.99 (2.77,3.22) , and modified Japanese Orthopedic Association (mJOA) scores were recorded for 208 patients, with a mean improvement of 3.41 (3.17,3.64) .

Conclusions.
These results support several advantages of spinal endoscopic techniques for the treatment of symptomatic TOLF. These include low complication rates, rapid postoperative recovery, and good functional recovery when used for single-segment, non-nodular ossification and no combined dural ossification.
A pig model of chronic hepatitis E displaying persistent viremia and a downregulation of innate immune responses in the liver
Authors: León-Janampa Nancy , Caballero-Posadas Ignacio , Barc Céline , Darrouzain François , Moreau Alain , Guinoiseau Thibault , Gatault Philippe , Fleurot Isabelle , Riou Mickaël , Pinard Anne , Pezant Jérémy , Rossignol Christelle , Gaudy-Graffin Catherine , Brand Denys , Marlet Julien 
Published: November 2023

Background:
Hepatitis E virus (HEV) is a zoonotic virus transmitted by pig meat and responsible for chronic hepatitis E in immunocompromised patients. It has proved challenging to reproduce this disease in its natural reservoir. We therefore aimed to develop a pig model of chronic hepatitis E to improve the characterization of this disease.

Methods:
Ten pigs were treated with a tacrolimus-based regimen and intravenously inoculated with HEV. Tacrolimus trough concentration, HEV viremia, viral diversity, innate immune responses, liver histology, clinical disease and biochemical markers were monitored for 11 weeks post-infection (p.i.).

Results:
HEV viremia persisted for 11 weeks p.i. HEV RNA was detected in the liver, small intestine, and colon at necropsy. Histological analysis revealed liver inflammation and fibrosis. Several mutations selected in the HEV genome were associated with compartmentalization in the feces and intestinal tissues, consistent with the hypothesis of extrahepatic replication in the digestive tract. Antiviral responses were characterized by a downregulation of IFN pathways in the liver, despite an upregulation of RIG-I and ISGs in the blood and liver.

Conclusions:
We developed a pig model of chronic hepatitis E that reproduced the major hallmarks of this disease. This model revealed a compartmentalization of HEV genomes in the digestive tract and a downregulation of innate immune responses in the liver. These original features highlight the relevance of our model for studies of the pathogenesis of chronic hepatitis E and for validating future treatments.
Development and clinical validation of a novel algorithmic score (GAAD) for detecting HCC in prospective cohort studies
Authors: Piratvisuth Teerha , Hou Jinlin , Tanwandee Tawesak , Berg Thomas , Vogel Arndt , Trojan Jörg , De Toni Enrico N. , Kudo Masatoshi , Eiblmaier Anja , Klein Hanns-Georg , Hegel Johannes Kolja , Madin Kairat , Kroeniger Konstantin , Sharma Ashish , Chan Henry L.Y. 
Published: November 2023

Background:
Alpha-fetoprotein (AFP) and des-gamma carboxyprothrombin (DCP), also known as protein induced by vitamin K absence-II (PIVKA-II [DCP]) are biomarkers for HCC with limited diagnostic value when used in isolation. The novel GAAD algorithm is an in vitro diagnostic combining PIVKA-II (DCP) and AFP measurements, age, and gender (biological sex) to generate a semi-quantitative result. We conducted prospective studies to develop, implement, and clinically validate the GAAD algorithm for differentiating HCC (early and all-stage) and benign chronic liver disease (CLD), across disease stages and etiologies.

Methods:
Patients aged ≥18 years with HCC or CLD were prospectively enrolled internationally into algorithm development [n = 1084; 309 HCC cases (40.7% early-stage) and 736 controls] and clinical validation studies [n = 877; 366 HCC cases (47.6% early-stage) and 303 controls]. Serum samples were analyzed on a cobas® e 601 analyzer. Performance was assessed using receiver operating characteristic curve analyses to calculate AUC.

Results:
For algorithm development, AUC for differentiation between early-stage HCC and CLD was 90.7%, 84.4%, and 77.2% for GAAD, AFP, and PIVKA-II, respectively. The sensitivity of GAAD for the detection of early-stage HCC was 71.8% with 90.0% specificity. Similar results were shown in the clinical validation study; AUC for differentiation between early-stage HCC and CLD was 91.4% with 70.1% sensitivity and 93.7% specificity. GAAD also showed strong specificity, with a lower rate of false positives regardless of disease stage, etiology, or region.

Conclusions:
The GAAD algorithm significantly improves early-stage HCC detection for patients with CLD undergoing HCC surveillance. Further phase III and IV studies are warranted to assess the utility of incorporating the algorithm into clinical practice.
Vitreous-lens interface changes after cataract surgery using active fluidics and active sentry with high and low infusion pressure settings
Authors: Scarfone Hugo A. , Rodriguez Emilia C. , Rufiner Maira G. , Riera José J. , Fanego Susel E. , Charles Martín , Albano Rodrigo 
Published: November 2023

Purpose:To determine whether the infusion pressure used during phacoemulsification may have a detrimental effect on the anterior hyaloid membrane barrier (AHMB) in a pressure fluctuation-free environment using diagnostic spectral-domain optical coherence tomography (SD-OCT) postoperatively.Setting:Tandil Eye Clinic, Tandil, Buenos Aires, Argentina, and Centro Oftalmológico Dr. Charles, CABA, Buenos Aires, Argentina.Design:Prospective, randomized, multicenter, experimental, and double-masked study.Methods:Phacoemulsification with intraocular lens implantation was performed in all patients with the Centurion Vision System equipment with active fluidics and active sentry. Patients were randomly assigned to configuration 1 or 2. Configuration 1 had intraocular pressure (IOP) 30 mm Hg and configuration 2 IOP 80 mm Hg. Inclusion criteria were axial length >22 mm and <25 mm, age older than 50 and younger than 70 years, and complete adhesion of AHMB.Results:80 eyes of 80 patients were included. Berger space was identified in 17 cases (42.5%) of group 2 and 3 cases (7.5%) of group 1 postoperatively using SD-OCT. A statistically significant relationship was established using Fisher exact test (P= .0003). Postoperatively, we observed posterior vitreous detachment changes in only 1 patient (1.25%) during the 3 months of follow-up (P= .5). According to the Wong-Baker FACES Scale, the patient's subjective perception was better for the low infusion pressure group (P= .0001, Fisher exact test).Conclusions:Phacoemulsification with high infusion pressure can change the vitreous-lens interface. Positive Berger space after phacoemulsification is a biomarker of this change and can occur in eyes without risk factors. Incidence is directly related to the infusion pressure used.
Electrode−Modiolus Distance Affects Speech Perception for Lateral Wall Electrodes
Authors: Ding Ling , Zhang Luguang , Li Jing , Lin Tao 
Published: November 2023

Objectives
The goal of this study was to use cone-beam computed tomography to locate the electrode–modiolus distance (EMD) and correlate this with speech perception in cochlear implant (CI) recipients of the 31.5-mm lateral wall (LW) electrode arrays.

Study Design
Retrospective review.

Patients
Forty-five child CI recipients with prelingual profound sensorineural hearing loss of inserted 31.5-mm LW arrays listening with a CI-alone device.

Interventions
Stepwise forward multiple linear regression was performed to control and reduce the variability in implant performance to determine whether EMD affects speech perception.

Main Outcome Measures
Electrode location (angular insertion depth [AID], EMD), together with the electrode impedance (EI), surgical approach, sex, CI age, and preimplant hearing aid usage were estimated as independent variables. The dependent variables were the Meaningful Use of Speech Scale (MUSS) and parents’ evaluation of children’s aural/oral performance (PEACH) assessed with the CI alone at 12 months postactivation.

Results
EMD and CI age were predictive variables for PEACH/MUSS. A negative correlation was found between AID and EMD (r = −0.56, p < 0.01), whereas EMD had a moderately positive correlation with EI (r = 0.32, p < 0.01).

Conclusions
The best “location-related” predictor of postoperative speech perception was EMD with a 31.5-mm array among CI-alone users.
Osteoprotegerin is associated with subclinical left ventricular systolic dysfunction in non-dipper hypertensive patients: a 2D speckle tracking echocardiographic study
Authors: Alizade Elnur , Kahyaoglu Muzaffer , Balaban Ismail , Izci Servet , Guler Ahmet 
Published: November 2023

Background
Past studies have shown that non-dipper hypertensive patients have more frequent subclinical left ventricular (LV) systolic dysfunction compared to dippers. Many different parameters have been examined to predict subclinical LV dysfunction. The role of osteoprotegerin (OPG) in the pathogenesis of heart failure and LV systolic dysfunction through different mechanisms had well described. In the present study, we hypothesized that increased OPG levels could predict subclinical LV systolic dysfunction in non-dipper hypertensive patients.

Patients and methods
Hypertensive patients were divided into two groups according to the results of ambulatory blood pressure (BP) monitoring. Non-dipper patients were subsequently divided into two further groups (normal LV function and impaired LV function) according to LV global longitudinal strain (GLS).

Results
A total of 103 hypertensive patients (51 dippers, 52 non-dippers) were included in the study. In the non-dipper group, LV GLS was normal in 21 patients and impaired in 31 patients. Based on the results of the multivariate logistic regression test, it was determined that OPG levels (OR: 2.413, 95% CI: 1.284–4.535, P = 0.006) and LVMI (OR: 1.086, 95% CI: 1.013–1.165, P = 0.021) were independently associated with impaired GLS.

Conclusion
Higher OPG values were associated with subclinical LV systolic dysfunction in non-dipper hypertensive patients. It could be used for the early diagnosis of subclinical LV systolic dysfunction, which would allow for strategies to be designed to reduce the cardiovascular event rate in this patient population.
Urea cycle disorders in critically Ill adults
Authors: Long Micah T. , Kruser Jacqueline M. , Quinonez Shane C. 
Published: November 2023

Purpose of reviewUrea cycle disorders (UCDs) cause elevations in ammonia which, when severe, cause irreversible neurologic injury. Most patients with UCDs are diagnosed as neonates, though mild UCDs can present later - even into adulthood - during windows of high physiologic stress, like critical illness. It is crucial for clinicians to understand when to screen for UCDs and appreciate how to manage these disorders in order to prevent devastating neurologic injury or death.Recent findingsHyperammonemia, particularly if severe, causes time- and concentration-dependent neurologic injury. Mild UCDs presenting in adulthood are increasingly recognized, so broader screening in adults is recommended. For patients with UCDs, a comprehensive, multitiered approach to management is needed to prevent progression and irreversible injury. Earlier exogenous clearance is increasingly recognized as an important complement to other therapies.SummaryUCDs alter the core pathway for ammonia metabolism. Screening for mild UCDs in adults with unexplained neurologic symptoms can direct care and prevent deterioration. Management of UCDs emphasizes decreasing ongoing ammonia production, avoiding catabolism, and supporting endogenous and exogenous ammonia clearance. Core neuroprotective and supportive critical care supplements this focused therapy.
A paradigm shift from office to home-based blood pressure measurement approaches in kidney transplant recipients
Authors: Tantisattamo Ekamol , Ferrey Antoney J. , Reddy Uttam G. , Malik Fatima T. , Siu Man Kit Michael , Ammary Fawaz Al , Lau Wei Ling 
Published: November 2023

Purpose of review
The unattended blood pressure (BP) readings from home blood pressure (HBP) monitoring should provide more accurate BP readings than attended BP obtained from office blood pressure (OBP). Here, we review evidence supporting the clinical utility of HBP and automatic remote monitoring of blood pressure (ARM-BP) in kidney transplant recipients (KTR).

Recent findings
BP from 24-h ambulatory blood pressure monitoring (24-h ABPM) is higher than but better associated with kidney and cardiovascular outcomes compared to OBP and HBP. While there is discordance of BP readings across different BP measurement methods causing BP misclassification, HBP provides BP readings closer to the readings from the 24-h ABPM than those from OBP. Systolic and diastolic BP is better controlled within 30 days after utilizing ARM-BP.

Summary
Compared to OBP, HBP minimizes the attended effect of OBP, and its readings are closer to the gold standard 24-h ABPM. ARM-BP improves BP control in the short term and trials of longer follow-up duration are required to evaluate sustained clinical benefits in KTR. The paradigm of BP monitoring may shift toward HBP, while OBP may be utilized primarily for KTR who cannot perform HBP for hypertension diagnosis and management.
The Mediating Effect of Hope Level Between Social Support and Benefit Finding in Patients With Advanced Lung Cancer
Authors: Zhao Mengjiao , Jiang Yunxia , Xu Guangyi , Lin Xin 
Published: November 2023

Background
As a protective factor, social support can influence benefit finding (BF) in patients with advanced lung cancer. However, the mechanism through which social support affects BF is controversial.

Objective
To investigate the factors influencing BF in patients with advanced lung cancer and the role of hope level in mediating the relationship between social support and BF.

Methods
This was a cross-sectional study. From September 2022 to March 2023, 286 participants completed the Social Support Rating Scale, Herth Hope Index, and Benefit Finding Scale. Data analysis included correlation analyses and multiple stepwise regression analysis; the SPSS PROCESS program was used to determine the significance of mediating effects.

Results
Social support and hope level were positively associated with BF. Monthly per-capita household income, social support and hope level entered the final regression model and explained 50.7% of BF variance. Social support’s direct and indirect effects on BF were significant (P < .05), suggesting that hope level partially mediated the relationship between social support and BF.

Conclusion
Our findings validate a positive association between social support and BF in patients with advanced lung cancer. The hope level as a mediating variable provided the impetus for their BF.

Implications for practice
Nurses should implement necessary interventions to help patients with advanced lung cancer carry out protective resources and coping strategies to facilitate their BF.
Can a Checklist Facilitate Recognition of a Transfusion-Associated Adverse Event by Prelicensure Nurses?
Authors: Scott Sue S. , Scanlon Marcia , McKelvey Michele , Nathanson Brian H. , Andrzejewski Chester 
Published: November 2023

Background:
Early identification of a transfusion-associated adverse event (TAAE) is key to patient safety. Research has indicated that use of a TAAE checklist resulted in greater frequency of TAAE recognition behaviors.

Purpose:
To explore whether use of a TAAE checklist resulted in greater frequency of TAAE recognition behaviors among nursing students.

Methods:
A prospective, randomized controlled study was conducted with 62 nursing students playing the role of a registered nurse in a simulation of a patient exhibiting clinical indicators of a TAAE. Subjects were randomly assigned to have or not have a TAAE checklist. Time to recognition of the TAAE was measured.

Results:
The checklist group (CG) identified TAAEs on average 4 minutes sooner than the non-CG (P < .001). CG subjects were also significantly less likely to miss TAAEs completely.

Conclusions:
A checklist may improve the safety of transfusions by promoting earlier recognition and treatment of TAAEs.