bluuebunny/crossref_metadata_2025_split huggingface dataset:
Has the concept of polyspermy prevention been invented in the laboratory?
Authors: Dale Brian 
Published: January 2024

SummaryThere is no evidence, nor need, for a fast block to polyspermy in animal oocytes. The idea that oocytes have evolved a mechanism to allow the entry of one spermatozoon and repel all others has, however, gained consensus over the last century. The main culprit is the sea urchin, which has been used for over a century in in vitro studies of the fertilization process. Images of sea urchin oocytes with thousands of sperm attached to the surface are commonplace in textbooks and appeal to the nature of the reader implying an intriguing surface mechanism of sperm selection despite these oocytes being fixed for photography (Figure 1). The abundance of gametes in this marine invertebrate and the ease of experimentation have given us the possibility to elucidate many aspects of the mechanism of fertilization, but has also led to ongoing controversies in reproductive biology, one being polyspermy prevention. Kinetic experiments by Rothschild and colleagues in the 1950s led to the hypothesis of a fast partial block to polyspermy in sea urchin oocytes that reduced the probability of a second spermatozoon from entering the oocyte by 1/20th. In the 1970s, Jaffe and colleagues suggested, with circumstantial evidence, that this partial block was due to the sperm-induced depolarization of the oocyte plasma membrane. However, the fate of supernumerary spermatozoa is determined well before the plasma membrane of the oocyte depolarizes. Transmembrane voltage does not serve to regulate sperm entry. Scholastic texts have inadvertently promulgated this concept across the animal kingdom with no logical correlation or experimentation and, as of today, a molecular mechanism to regulate sperm entry in oocytes has not been identified.
Impact of early postoperative haemodynamic and laboratory parameters on outcome after the Fontan procedure
Authors: Di Padua Chiara , Osawa Takuya , Waschulzik Birgit , Balling Gunter , Schaeffer Thibault , Staehler Helena , Piber Nicole , Hager Alfred , Ewert Peter , Hörer Jürgen , Ono Masamichi 
Published: January 2024

AbstractObjective:To identify early postoperative haemodynamic and laboratory parameters predicting outcomes following total cavopulmonary connection.Methods:Patients who underwent total cavopulmonary connection between 2012 and 2021 were evaluated. Serial values of mean pulmonary artery pressure, mean arterial pressure, peripheral oxygen saturation, and lactate levels were collected. The influence of these variables on morbidities was analyzed. Cut-off values were calculated using the receiver operating characteristic analysis.Results:A total of 249 patients were included. All patients had previous bidirectional cavopulmonary shunt. Median age and weight at total cavopulmonary connection were 2.2 (1.8–2.7) years and 11.7 (10.7–13.4) kg, respectively. All patients were extubated in the ICU at a median of 3 (2–5) hours after ICU admission. Postoperative pulmonary artery pressure, around 12 hours after extubation, was significantly associated with chest tube drainage (p = 0.048), chylothorax (p = 0.021), ascites (p = 0.016), and adverse events (p = 0.028). Receiver operating characteristic analysis revealed a cut-off value of 13–15 mmHg for chest tube drainage and chylothorax and 17 mmHg for ascites and adverse events. Mean arterial pressure 1 hour after extubation was associated with prolonged chest tube drainage (p = 0.015) and adverse events (p = 0.008). Peripheral oxygen saturation 6 hours after extubation (p = 0.003) was associated with chest tube duration and peripheral oxygen saturation 1 hour after extubation (p < 0.001) was associated with ascites. Lactate levels on 2nd postoperative day (p = 0.022) were associated with ascites and lactate levels on 1st postoperative day (p = 0.009) were associated with adverse events.Conclusions:Higher pulmonary artery pressure, lower mean arterial pressure, lower peripheral oxygen saturation, and higher lactate in early postoperative period, around 12 hours after extubation, predicted in-hospital and post-discharge adverse events following total cavopulmonary connection.
Development of robust normative data for the neuropsychological assessment of Greek older adults
Authors: Arampatzi Xanthi , Margioti Eleni S. , Messinis Lambros , Yannakoulia Mary , Hadjigeorgiou Georgios , Dardiotis Efthimios , Sakka Paraskevi , Scarmeas Nikolaos , Kosmidis Mary H. 
Published: January 2024

AbstractObjective:Normative data for older adults may be tainted by inadvertent inclusion of undiagnosed individuals at the very early stage of a neurodegenerative process. To avoid this pitfall, we developed norms for a cohort of older adults without MCI/dementia at 3-year follow-up.Methods:A randomly selected sample of 1041 community-dwelling individuals (age ≥ 65) received a full neurological and neuropsychological examination on two occasions [mean interval = 3.1 (SD = 0.9) years].Results:Of these, 492 participants (Group 1; 65–87 years old) were without dementia on both evaluations (CDR=0 and MMSE ≥ 26); their baseline data were used for norms development. Group 2 (n = 202) met the aforementioned criteria only at baseline, but not at follow-up. Multiple linear regressions included demographic predictors for regression-based normative formulae and raw test scores as dependent variables for each test variable separately. Standardized scaled scores and stratified discrete norms were also calculated. Group 2 performed worse than Group 1 on most tests (p-values < .001–.021). Education was associated with all test scores, age with most, and sex effects were consistent with the literature.Conclusions:We provide a model for developing sound normative data for widely used neuropsychological tests among older adults, untainted by potential early, undiagnosed cognitive impairment, reporting regression-based, scaled, and discrete norms for use in clinical settings to identify cognitive decline in older adults. Additionally, our co-norming of a variety of tests may enable intra-individual comparisons for diagnostic purposes. The present work addresses the challenge of developing robust normative data for neuropsychological tests in older adults.
Premorbid personality traits as predictors for incident predementia syndromes: a multistate model approach
Authors: Schaeffer Morgan J. , MacDonald Stuart W.S. , Paterson Theone S.E. 
Published: January 2024

AbstractObjective:Associations have been found between five-factor model (FFM) personality traits and risk of developing specific predementia syndromes such as subjective cognitive decline (SCD) and mild cognitive impairment (MCI). The aims of this study were to: 1) Compare baseline FFM traits between participants who transitioned from healthy cognition or SCD to amnestic MCI (aMCI) versus non-amnestic MCI (naMCI); and 2) Determine the relationship between FFM traits and risk of transition between predementia cognitive states.Methods:Participants were 562 older adults from the Einstein Aging Study, 378 of which had at least one follow-up assessment. Baseline data collected included levels of FFM personality traits, anxiety and depressive symptoms, medical history, performance on a cognitive battery, and demographics. Follow-up cognitive diagnoses were also recorded.Results:Mann–Whitney U tests revealed no differences in baseline levels of FFM personality traits between participants who developed aMCI compared to those who developed naMCI. A four-state multistate Markov model revealed that higher levels of conscientiousness were protective against developing SCD while higher levels of neuroticism resulted in an increased risk of developing SCD. Further, higher levels of extraversion were protective against developing naMCI.Conclusions:FFM personality traits may be useful in improving predictions of who is at greatest risk for developing specific predementia syndromes. Information on these personality traits could enrich clinical trials by permitting trials to target individuals who are at greatest risk for developing specific forms of cognitive impairment. These results should be replicated in future studies with larger sample sizes and younger participants.
An overview of the nutritional status of childbearing age women, children and adolescents living in a rural area of Madagascar: preliminary results of the Tany Vao project
Authors: Conti Maria Vittori , Itani Leila , Beretta Alice , Bono Edoardo , Yaghi Kassandra , Filosa Asia , Monti Cristina , Cena Hellas 
Published: January 2024

Abstract

Objective:
To describe the food consumption, nutrition knowledge and nutritional assessment of childbearing age women and their children, living in rural villages in Madagascar. The results presented are related to the Tany Vao research study.

Design:
A cross-sectional pilot study.

Setting:
The study was carried out in Ampanitosoha village on Nosy Mitsio island in Madagascar.

Participants:
32 women (14–49 years) and 36 children and adolescents (2–17 years).

Results:
70 % of the women lacked nutrition knowledge and did not reach the Minimum Dietary Diversity Index for Women cut-off. The median BMI was 21·1 kg/m2 but 55·2 % of the women exceeded the cut-off for waist-to-hip ratio, 51·7 % for waist-to-height ratio and 81·2 % for mid-upper arm circumference (MUAC). Almost all had adequate intake of energy, protein and carbohydrates, while 27·6 % had excessive fat intake and 75·9 % of added sugars. Over half of the women did not meet the micronutrients Reference Daily Intake (RDI). For children, the MUAC z-score was lower for boys than for girls (P-value = 0·041).

Conclusions:
These results underline the importance of increasing women’s nutritional knowledge to promote healthy pregnancy and lactation. Moreover, it is fundamental to provide people living in rural areas with sustainable tools to improve dietary diversity and support long-term health.
Through the looking glass: the effect of participation in a participatory budget on citizens’ populist attitudes
Authors: Theuwis Marie-Isabel , Kindt Rosa 
Published: January 2024

AbstractMany citizens feel excluded from political decision-making, which, in their eyes, is dominated by an unresponsive political elite. Citizens with high populist attitudes perceive the world through a populist ‘lens’ and therefore yearn for more popular control and for ‘the people’ to be included in the political process. Participatory budgeting should be particularly suited to address populist demands due to the fact that it is focused on giving citizens actual influence on policy-making. However, so far, no study has examined the effect of participation in a democratic innovation on populist attitudes. This paper empirically assesses if and to what extent participation in a participatory budget affects populist attitudes, and whether citizens with high populist attitudes are affected differently than citizens with low populist attitudes. We analyze panel data on participants of four local participatory budgeting events in the Netherlands before and after participation and find that citizens with high populist attitudes decrease these attitudes significantly after participating in a participatory budget, whereas citizens with low populist attitudes are not significantly affected. Moreover, the significant difference in change between these two groups suggests that citizens with high populist attitudes go ‘through the looking glass’ and become less populist after participating in a participatory budget.
Weed communities after decades of mineral fertilization and tillage treatments in a corn–soybean rotation
Authors: Simard Marie-Josée , Ziadi Noura 
Published: January 2024

Abstract
Soil fertility and disturbance can potentially modify weed dynamics in a corn–soybean rotation. Knowing how added mineral fertilizers and tillage influence weed populations in the long term can provide insight into weed community shifts, which may affect future weed management requirements. The first objective of this study was to evaluate the effect of 24 to 25 years of nitrogen and phosphorus fertilization treatments and tillage (moldboard vs. no-till) on weeds (density, biomass, and composition) before and after herbicide applications in 2016/corn and 2017/soybean. The second objective was to evaluate the effect of the same treatments 6 yr after no postemergence weed management in 2022/corn. Since the last evaluation performed in 2004, weed density, richness, and diversity increased, and more annual grasses were observed under both tillage regimes. No fertilization effect was observed on any weed variables, including composition, except for increased biomass when left to grow all season after crop planting. In managed plots, the density and biomass of annuals and perennials were generally higher in no-till, and discrepancies were highest for annual grass densities before herbicide application. Weed species richness and diversity based on counts were equivalent between tillage regimes, but total biomass was distributed between more species in no-till. Higher weed densities and concurrent weed biomass, observed in no-till, reduced crop yields in 2016/soybean only. The combination of low crop diversity and low use of residual herbicides during the trial potentially led to the observed species shifts and increased weed density.
Adaptations in wild radish (Raphanus raphanistrum) flowering time, Part 2: Harvest weed seed control shortens flowering by twelve days
Authors: Somerville Gayle J. , Ashworth Michael B. 
Published: January 2024

AbstractHarvest weed seed control (HWSC) is an effective technique for managing wild radish (Raphanus raphanistrum L.), a weed that retains its seed until harvest. However, earlier flowering time (leading to increased seed shedding before harvest) is a risk to HWSC effectiveness. This study investigated the effects of repeated HWSC on the evolution of R. raphanistrum flowering dates, using two methods: an adaptation of the SOMER model that included flowering genes (called SOMEF); and a mathematical calculation of the endpoints of flowering date evolution utilizing the relevant life-history equations. In weed management systems with highly effective herbicides, the additional use of HWSC predicted R. raphanistrum population extinction. Low weed numbers and rapid extinction meant that any gradual evolution in days to first flower (DFF) was insufficient to lead to HWSC evasion. In alternative management systems with less vigorous herbicide control and using HWSC, modeling predicted a maximum 2- to 3-d reduction in DFF. In contrast, mathematical calculations of the phenotypes maximizing seeds returned to the seedbank predicted an endpoint to evolution of 12-d earlier flowering, which matched field observations. However, genetic change postulated by the mathematical calculations was not hampered by a restriction to changing DFF allele frequencies. Unknown accompanying genetic changes could affect germination dates or flowering triggers.Simulation modeling that included only flowering genes failed to predict the magnitude of an observed 12-d reduction in DFF. Differences between the 12 d observed in the field (and predicted using mathematical calculations) and the modest changes demonstrated in this field-based modeling study are postulated to be due to unaccounted evolutionary changes in R. raphanistrum.
Laboratory Hydrofractures as Analogs to Tectonic Tremors
Authors: Yuan C. , Cochard T. , Denolle M. , Gomberg J. , Wech A. , Xiao L. , Weitz D. 
Published: January 2024

AbstractThe fracture of Earth materials occurs over a wide range of time and length scales. Physical conditions, particularly the stress field and Earth material properties, may condition rupture in a specific fracture regime. In nature, fast and slow fractures occur concurrently: tectonic tremor events are fast enough to emit seismic waves and frequently accompany slow earthquakes, which are too slow to emit seismic waves and are referred to as aseismic slip events. In this study, we generate simultaneous seismic and aseismic processes in a laboratory setting by driving a penny‐shaped crack in a transparent sample with pressurized fluid. We leverage synchronized high‐speed imaging and high‐frequency acoustic emission (AE) sensing to visualize and listen to the various sequences of propagation (breaks) and arrest (sticks) of a fracture undergoing stick‐break instabilities. Slow radial crack propagation is facilitated by fast tangential fractures. Fluid viscosity and pressure regulate the fracture dynamics of slow and fast events, and control the inter‐event time and the energy released during individual fast events. These AE signals share behaviors with observations of episodic tremors in Cascadia, United States; these include: (a) bursty or intermittent slow propagation, and (b) nearly linear scaling of radiated energy with area. Our laboratory experiments provide a plausible model of tectonic tremor as an indicative of hydraulic fracturing facilitating shear slip during slow earthquakes.
A Systems Analysis of Sustainability Impacts of Agricultural Policies in India
Authors: Maji P. , Selin N. E. 
Published: January 2024

AbstractWe apply a systems framework for analyzing the overall sustainability impacts of interventions to a case of the rice‐wheat cropping system of Punjab (India), where agricultural practices lead to air pollution‐related health impacts, over‐exploitation of groundwater, over‐use of fertilizers and reduced local crop diversity. We use this case to quantify how varying degrees of change in interventions result in sustainability impacts using an inclusive wealth‐based approach. We show that either improving the existing cropping system or inducing fundamental changes in the cropping system, can lead to substantial and wide‐ranging sustainability benefits. We also show that interventions that improve human health show the largest quantitative benefit due to the assumed high marginal value of human life. Accurate localized estimates of marginal values of stocks are needed for estimating overall sustainability impacts.
Roles of Continental Mid‐Lithosphere Discontinuity in the Craton Instability Under Variable Tectonic Regimes
Authors: Fu Hui‐Ying , Li Zhong‐Hai 
Published: January 2024

AbstractThe continental mid‐lithosphere discontinuity (MLD) is widely detected within cratons, with the dominant depth range of 70–100 km and a significant reduction of shear‐wave velocity of 2%–12%. However, the formation mechanism and corresponding strength of the MLD are widely debated, which may strongly affect the roles of MLD in craton evolution. The comparisons among variable mechanisms indicate that the strength of the MLD varies from the relatively high viscosity of wet olivine to the rather low viscosity of antigorite. Thus, systematic numerical modeling has been conducted with the MLD of contrasting strengths, that is, the wet olivine‐induced MLD or antigorite‐induced MLD, to investigate the roles of MLD in the craton instability under variable tectonic regimes (stable, extension, compression, mantle flow traction, or mantle plume). The models show that the cratonic lithosphere with wet olivine‐induced MLD maintains its stability under all the tectonic regimes. In contrast, the antigorite‐induced MLD with lowest viscosity could significantly promote the decoupling of lithosphere, and facilitate the lithospheric deformation. However, lithospheric delamination only occurs with the rather weak MLD interacting with the sub‐plate asthenosphere upwelling during craton extension or mantle plume activity. The sufficient amount of melts is essential for this process, which requires a large amount of extension or a mantle plume with rather high temperature anomaly and large size. Therefore, craton destruction is still difficult, and requires additional strict conditions. This may explain the general stability of most cratons with widespread MLDs.
Determinants of Field‐Saturated Soil Hydraulic Conductivity Across Sub‐Saharan Africa: Texture and Beyond
Authors: Bargués‐Tobella Aida , Winowiecki Leigh Ann , Sheil Douglas , Vågen Tor‐Gunnar 
Published: January 2024

AbstractSoil infiltration is critical for water security and related ecosystem services. This infiltration, the ability of soils to absorb water at their surface, is controlled by the soil hydraulic conductivity. Despite recent efforts in assembling measurements of soil hydraulic conductivity, global databases and derived pedotransfer functions lack coverage in the tropics. Here, we present soil infiltration measurements and other indicators of soil and land health collected systematically in 3,573 plots from 83 100 km2 sites across 19 countries in sub‐Saharan Africa. We use these data to (a) determine field‐saturated hydraulic conductivity (K
fs) and (b) explore which variables best predict variation in K
fs. Our results show that sand content, soil organic carbon (SOC), and woody cover had a positive relationship with K
fs, whereas grazing intensity and soil pH had a negative relationship. Our findings highlight that, despite soil texture being important, structure also plays a critical role. These results indicate considerable potential to improve soil hydrological functioning through management and restoration practices that target soil structure. Enhancing SOC content, limiting animal stocking, promoting trees, shrubs, and other vegetation cover, and preventing soil erosion can increase K
fs and improve water security. This data set can contribute to improving Earth system and land surface models for applications in Africa.
Increase in peg-asparaginase clearance as a predictor for inactivation in patients with acute lymphoblastic leukemia
Authors: Dam Merete , Centanni Maddalena , Friberg Lena E. , Centanni Daniel , Karlsson Mats O. , Stensig Lynggaard Line , Johannsdottir Inga Maria , Wik Hilde Skuterud , Malmros Johan , Vaitkeviciene Goda Elizabeta , Griskevicius Laimonas , Hallböök Helene , Jónsson Ólafur Gísli , Overgaard Ulrik , Schmiegelow Kjeld , Hansen Stefan Nygaard , Heyman Mats , Albertsen Birgitte Klug 
Published: January 2024

AbstractAsparaginase is an essential component of acute lymphoblastic leukemia (ALL) therapy, yet its associated toxicities often lead to treatment discontinuation, increasing the risk of relapse. Hypersensitivity reactions include clinical allergies, silent inactivation, or allergy-like responses. We hypothesized that even moderate increases in asparaginase clearance are related to later inactivation. We therefore explored mandatory monitoring of asparaginase enzyme activity (AEA) in patients with ALL aged 1–45 years treated according to the ALLTogether pilot protocol in the Nordic and Baltic countries to relate mean AEA to inactivation, to build a pharmacokinetic model to better characterize the pharmacokinetics of peg-asparaginase and assess whether an increased clearance relates to subsequent inactivation. The study analyzed 1631 real-time AEA samples from 253 patients, identifying inactivation in 18.2% of the patients. This inactivation presented as mild allergy (28.3%), severe allergy (50.0%), or silent inactivation (21.7%). A pharmacokinetic transit compartment model was used to describe AEA-time profiles, revealing that 93% of patients with inactivation exhibited prior increased clearance, whereas 86% of patients without hypersensitivity maintained stable clearance throughout asparaginase treatment. These findings enable prediction of inactivation and options for either dose increments or a shift to alternative asparaginase formulations to optimize ALL treatment strategies.