bluuebunny/crossref_metadata_2025_split huggingface dataset:
The fatty acid composition of black soldier fly larvae: the influence of feed substrate and applications in the feed industry
Authors: Cattaneo A. , Meneguz M. , Dabbou S. 
Published: November 2023

Abstract
The need to reduce, reuse and recycle materials by applying new strategies of circular economy instead of linear systems of disposal is becoming increasingly urgent. The black soldier fly (BSF), Hermetia illucens (L.) (Diptera: Stratiomyidae), is being studied for its outstanding capacity to convert organic waste into high-value biomass and as a valuable nutrient source for animal feed. A systematic literature review identified 35 articles on BSF rearing and use in animal feed. This review summarizes our current knowledge about the lipid composition of BSF larvae, in particular their fatty acid (FA) profile and the mechanisms through which the feeding substrate influences the FA profile. BSF larvae are shown to synthesize certain FAs, such as lauric and myristic acid, even when these components are absent from their diet. Furthermore, enriching the rearing substrate with eicosapentaenoic and docosahexaenoic acid is reported to increase the accumulation of these FAs. The saturated FA content increases when BSF larvae are reared on a vegetable-based diet. Moreover, enriching the rearing media with materials high in omega-3 and omega-6 FAs increases their concentration in the larvae and enhances their value as a feed substrate for other animals. Indeed, the supplementation of animal feeds with BSF larvae products is being studied for application in the aquaculture, poultry, and pig farming sectors. BSF larval fats are shown to have a positive effect on animal growth performances, body composition, and gut histology, establishing BSF larvae as an environmentally-sustainable feed ingredient. This review highlights the importance of deepening our knowledge on the various aspects of BSF larvae rearing and treatments which enhance the accumulation of specific lipids and minimize lipid quality deterioration and loss. By summarizing the existing literature on BSF larvae, this article contributes to the advancement of circular economies and the development of sustainable feed practices.
Steam pre-treatment of sugarcane bagasse and wheat straw as a cleaner feedstock for black soldier fly larvae rearing
Authors: Theron W. , Teke G.M. , Diedericks D. , Van Rensburg E. , Görgens J.F. 
Published: November 2023

Abstract
With the pressing need for alternative waste management strategies that are friendly to the environment, black soldier fly larvae (BSFL) are being cultivated as exceptional insects for the bioconversion of organic waste into larval biomass rich in protein and fat content. Agricultural residues, such as wheat straw and sugarcane bagasse are recognised as important renewable biomass sources, with potential to replace insufficient amount of suitable organic wastes available for BSFL feeding. This study evaluated the steam pre-treatment from 140 to 215 °C and enzymatic hydrolysis of lignocelluloses as BSFL feed supplement prior to blending in an equal ratio (50:50) with the standard feed for BSFL rearing. Key findings illustrated that steam pre-treatment and enzymatic hydrolysis are vital in liberating the sugar monomers of the lignocellulose biomass for BSFL utilisation, with an optimum steam pre-treatment temperature of 185 °C. With a lignocellulosic feed prepared at this temperature plus supplemented standard feed, a BSFL dry-weight bioconversion of 16% was achieved in 8 to 11 days of rearing. The inhibitory by-products formed by degradation of lignocelluloses during pre-treatment had a significantly negative effect on the rearing of the BSFL, particularly a furan concentration of 0.2 g/L, which should be investigated for efficient BSFL rearing. Finally, the crude protein content in the BSFL reared on the standard feed was higher than the feed substrate supplemented with lignocellulose. This study demonstrated the need for process optimisation and component supplements in the BSFL feed substrates that contain lignocellulosic feedstocks.
Optimization models for sustainable insect production chains
Authors: Mouhrim N. , Peguero D.A. , Green A. , Silva B. , Bhatia A. , Ristic D. , Tonda A. , Mathys A. , Smetana S. 
Published: November 2023

Abstract
Insect value chains are a complex system with non-linear links between many economic, environmental, and social variables. Multi-objective optimization (MOO) algorithms for finding optimal options for complex system functioning can provide a valuable insight in the development of sustainable insect chains. This review proposes a framework for MOO application that is based on gradual implementation, beginning with factors that have an immediate impact on insect production (feed qualities, resource utilization, yield), and progressing to integrated units (environmental, social, and economic impacts). The review introduces the key hotspots of insect production chains, which have been developed in suitable MOO objectives. They represent aspects of resource use, feed quality and its conversion by insects, labor safety and wage fairness, as well as environmental impacts. The capacity of the suggested MOO framework to describe all facets of sustainability may have certain limits. To determine the framework’s applicability and the specific MOO algorithms that can perform the function, modeling and further testing on real insect production chains would be necessary for the intended objectives.
Positioning earthworms in the future foods debate: a systematic review of earthworm nutritional composition in comparison to edible insects
Authors: Sonntag E. , Vidal A. , Grimm D. , Rahmann G. , van Groenigen J.W. , van Zanten H. , Parodi A. 
Published: November 2023

Abstract
Sustainable food system innovations are urgently needed to feed a growing human population while staying within planetary boundaries. Farmed edible insects have received considerable scientific and public attention due to their potential to improve food system circularity by upcycling nutrients from organic residual streams to nutritious food. Earthworms, as non-insect invertebrates, have remained largely unrecognized in the future foods debate. However, they are already widely farmed at industrial scale for their capacity to recycle organic wastes and improve soil fertility. We conducted a systematic literature review to provide a quantitative basis on earthworm nutritional composition, thereby positioning earthworms in the future foods debate. Here we show, based on evidence from 142 scientific studies, that farmed earthworms are a potentially interesting food source. They have an attractive nutrient composition compared to the main farmed edible insect species, being especially rich in protein, low in fat and containing a favourable profile of essential amino acids. The content of important fatty acids, minerals and vitamins in earthworm biomass is higher or lower than in edible insects, depending on the feed material. Crude protein and fat contents are higher in farmed versus wild earthworms, indicating that farming conditions provide a lever for further improving the nutritional composition of earthworm biomass. Whether earthworm species or feed materials affect earthworm nutritional composition could not be finally clarified based on the available data. We conclude that earthworms have high potential as a future food from a nutritional perspective, mainly as an alternative source of protein. The integration of earthworm farming in future food systems can be expected to improve sustainability and circularity, potentially giving earthworms an advantage over edible insects.
Investigation of the usefulness of two-spotted cricket (Gryllus bimaculatus) feed using two-spotted cricket (Gryllus bimaculatus) powder to replace fishmeal
Authors: Akiyama D. , Kaewplik T. , Sasaki Y. 
Published: November 2023

Abstract
Crickets are being reevaluated as food and livestock feed due to their high nutritional value and low environmental impact during production, as food waste can be used as feed. On the other hand, current cricket production uses feed used in aquaculture and poultry farming, and these feeds contain animal proteins such as fish meal and chicken meal. This is simply converting other animal proteins into insect proteins. Therefore, this study aimed to test whether two-spotted cricket powder can be used as a substitute for fishmeal in diets for Gryllus bimaculatus production. Cricket weight, feed efficiency, and number of crickets were evaluated by feeding rice bran: cricket powder = 85:15 and rice bran: fish meal = 85:15, respectively, referring to the feed mix ratio used by cricket producers. Results showed no statistically significant differences between the two experiments in cricket weight and feed conversion ratio. When data were obtained separately for each growth phase, cricket weight was found to be highest in the fourth post-hatching week for the diet with cricket powder and then decreased (initial input: 1 g; 28 days: 89.45 ± 22.19 g; 35 days: 49.47 ± 14.11 g). Feed conversion efficiency was found to be higher in the second half of growth (1-7 days: 0.48; 23-28 days: 0.80). The use of insect powder as bait for crickets is a new proposal. In cricket production, adults with low reproductive capacity are packaged and sold as food or livestock feed, but by using insect powder as feed during cricket production, it is possible to reduce animal protein in the feed. In the future, we plan to investigate the usefulness of mass-produced insect powder as feed for crickets.
Envisioning the Revolution: Art and the Creation of Mexico’s Communist Party
Authors: Smith Stephanie J. 
Published: November 2023

Abstract
This article analyzes the interactions of artists, Mexico’s Communist Party, or the Partido Comunista Mexicano (pcm), and the Mexican state within the context of Mexico’s vibrant post-revolutionary era. Although during these early years the Party’s official membership numbers remained relatively minimal, this article argues that the extraordinary influence of Mexico’s creative participants on the politics of the period was significant. During the 1920s the pcm derived a great deal of prestige from its association with art and the muralists, including Diego Rivera and David Alfaro Siqueiros, and as a result Mexico’s lively political and artistic scene attracted the attention of writers, photographers, artists, and intellectuals from all over the world. Art and politics intertwined as artists played major roles in political affairs, and politicians appropriated the arts to transmit the “official” national history. Indeed, during these exhilarating years, the artists and the pcm built a powerful coalition, and one whose influence endured long beyond the 1920s.
The Standard Language Ideology of the Hebrew and Arabic Grammarians of the ʿAbbasid Period
Authors: Kantor Benjamin Paul 
Published: November 2023

As a discipline, the study of Biblical Hebrew grammar began largely among Arabic-speaking Jews of the Middle Ages, particularly in the ʿAbbasid period (750–1258 CE). Indeed, it has long been acknowledged by scholars that the Hebrew grammatical tradition, in many ways, grew up out of and alongside the Arabic grammatical tradition. Many concepts present in Hebrew grammar have their origins in the writings of Arabic grammarians of the ʿAbbasid period. And yet, as recent linguistic and anthropological work has shown, setting down ‘the grammar’ of a language can be as much an ideological or political activity as an academic one.
In addition to the language itself, speech communities also share beliefs and attitudes about that language—what linguistic anthropologists would term a ‘language ideology’. Language ideology can have a dramatic impact on what forms of the language one regards as acceptable and what sort of rules one imposes on and through their description of the language. Nevertheless, while much work has been done on the interface between Hebrew and Arabic grammar and literature in the Middle Ages, interface of their respective language ideologies has yet to be treated theoretically or systematically.
In the present book, then, we survey six specific characteristics of a ‘standard language ideology’ that appear in both the writings of the Hebrew grammarians who wrote in Judeo-Arabic and the Arabic grammarians during the ʿAbbasid period. Such striking lines of linguistic-ideological similarity suggest that it may not have been only grammatical concepts or literary genres that the medieval Hebrew grammarians inherited from the Arabic grammatical tradition, but a way of thinking about language as well.
Mechanisms of Offshore Solid and Liquid Freshwater Flux from the East Greenland Current
Authors: Spall Michael A. , Semper Stefanie , Våge Kjetil 
Published: November 2023

Abstract
The mechanisms that control the export of freshwater from the East Greenland Current, in both liquid and solid form, are explored using an idealized numerical model and scaling theory. A regional, coupled ocean–sea ice model is applied to a series of calculations in which key parameters are varied and the scaling theory is used to interpret the model results. The offshore ice flux, occurring in late winter, is driven primarily by internal stresses and is most sensitive to the thickness of sea ice on the shelf coming out of Fram Strait and the strength of alongshore winds over the shelf. The offshore liquid freshwater flux is achieved by eddy fluxes in late summer while there is an onshore liquid freshwater flux in winter due to the ice–ocean stress, resulting in only weak annual mean flux. The scaling theory identifies the key nondimensional parameters that control the behavior and reproduces the general parameter dependence found in the numerical model. Climate models predict that winds will increase and ice export from the Arctic will decrease in the future, both of which will lead to a decrease in the offshore flux of sea ice, while the influence on liquid freshwater may increase or decrease, depending on the relative changes in the onshore Ekman transport and offshore eddy fluxes. Additional processes that have not been considered here, such as more complex topography and synoptic wind events, may also contribute to cross-shelf exchange.

Significance Statement
The purpose of this study is to provide a basic understanding of what controls the flux of sea ice and low-salinity water from the East Greenland shelf into the interior of the Greenland and Iceland Seas. This is a potentially important process since it has been shown that sufficient freshening of the surface waters in the interior of the Nordic seas can inhibit deep convection and the associated air–sea heat flux and water mass transformation. A combination of idealized computer models and basic theory indicates that the fluxes of liquid and solid freshwater are controlled by different mechanisms and occur at different times of the year. Accurate representation in climate models will require representation of small-scale processes such as mesoscale eddies and gradients of ice thickness across the shelf.
FastCAR: fast correction for ambient RNA to facilitate differential gene expression analysis in single-cell RNA-sequencing datasets
Authors: Berg Marijn , Petoukhov Ilya , van den Ende Inge , Meyer Kerstin B. , Guryev Victor , Vonk Judith M. , Carpaij Orestes , Banchero Martin , Hendriks Rudi W. , van den Berge Maarten , Nawijn Martijn C. 
Published: November 2023

AbstractCell type-specific differential gene expression analyses based on single-cell transcriptome datasets are sensitive to the presence of cell-free mRNA in the droplets containing single cells. This so-called ambient RNA contamination may differ between samples obtained from patients and healthy controls. Current ambient RNA correction methods were not developed specifically for single-cell differential gene expression (sc-DGE) analyses and might therefore not sufficiently correct for ambient RNA-derived signals. Here, we show that ambient RNA levels are highly sample-specific. We found that without ambient RNA correction, sc-DGE analyses erroneously identify transcripts originating from ambient RNA as cell type-specific disease-associated genes. We therefore developed a computationally lean and intuitive correction method, Fast Correction for Ambient RNA (FastCAR), optimized for sc-DGE analysis of scRNA-Seq datasets generated by droplet-based methods including the 10XGenomics Chromium platform. FastCAR uses the profile of transcripts observed in libraries that likely represent empty droplets to determine the level of ambient RNA in each individual sample, and then corrects for these ambient RNA gene expression values. FastCAR can be applied as part of the data pre-processing and QC in sc-DGE workflows comparing scRNA-Seq data in a health versus disease experimental design. We compared FastCAR with two methods previously developed to remove ambient RNA, SoupX and CellBender. All three methods identified additional genes in sc-DGE analyses that were not identified in the absence of ambient RNA correction. However, we show that FastCAR performs better at correcting gene expression values attributed to ambient RNA, resulting in a lower frequency of false-positive observations. Moreover, the use of FastCAR in a sc-DGE workflow increases the cell-type specificity of sc-DGE analyses across disease conditions.
Identification of hypervirulent Klebsiella pneumoniae based on biomarkers and Galleria mellonella infection model
Authors: Mai Dongmei , Wu Anqi , Li Ran , Cai Donghao , Tong Huichun , Wang Nan , Tan Junqing 
Published: November 2023

Abstract
Background
Currently, clinical laboratories lack an effective method to differentiate between classical Klebsiella pneumoniae (cKP) and hypervirulent Klebsiella pneumoniae (hvKP) strains, leading to delays in diagnosing and treating hvKP infections. Previous studies have identified peg-344, iroB, iucA, prmpA, prmpA2, and siderophores (SP) yields greater than 30 μg/ml as reliable markers for distinguishing hvKP from cKp strains. However, these diagnostic tests were conducted on a relatively small study population and lacked sufficient clinical data support. In this study, hvKP strains were identified by biomarker analysis and the Galleria mellonella model. Combined with in vitro and in vivo experiments, the reliability of clinical identification method of hvKP was verified, which provided an experimental basis for timely diagnosis of hvKP infection.

Results
According to the clinical data, a total of 108 strains of hvKP were preliminary screened. Among them, 94 strains were further identified using PCR analysis of biomarkers and quantitative determination of SP. The high virulence of hvKP was subsequently confirmed through infection experiments on Galleria mellonella. Additionally, susceptibility testing revealed the identification of 58 carbapenem-resistant hvKP (CR-hvKP) strains and 36 carbapenem-sensitive hvKP (CS-hvKP) strains. By comparing molecular diagnostic indexes, molecular characteristics such as high SP production of CR-hvKP were found.

Conclusion
The combination of clinical data and molecular diagnostic index analysis effectively enables the identification of hvKP, particularly CR-hvKP. This study provides a scientific basis for accurate clinical identification and timely treatment of hvKP.
A novel report on the emerging and zoonotic neurotropic fungus Trichosporon japonicum in the brain tissue of the endangered Brazilian guitarfish (Pseudobatos horkelii) off the southeastern coast of Brazil
Authors: Peixoto-Rodrigues Maria Carolina , da Costa Gisela Lara , Pinto Tatiane Nobre , Adesse Daniel , Oliveira Manoel Marques Evangelista , Hauser-Davis Rachel Ann 
Published: November 2023

AbstractYeast infections have gained significant attention in the field of marine biology in recent years. Among the broad diversity of marine organisms affected by these infections, elasmobranchs (sharks and rays) have emerged as highly susceptible, due to climate change effects, such as increasing water temperatures and pollution, which can alter the composition and abundance of fungal communities. Additionally, injuries, or compromised immune systems resulting from pollution or disease may increase the likelihood of fungal infections in elasmobranchs. Studies are, however, still lacking for this taxonomic group. In this context, this study aimed to screen yeast species in cell cultures obtained from the brain of artisanally captured Pseudobatos horkelii, a cartilaginous fish that, although endangered, is highly captured and consumed worldwide. Fungi were isolated during an attempt to establish primary cultures of elasmobranch neural cells. Culture flasks were swabbed and investigated using morphological, phenotypic, and molecular techniques. Two isolates of the emerging opportunistic pathogen Trichosporon japonicum were identified, with high scores (1.80 and 1.85, respectively) by the MALDI-ToF technique. This is the first report of the basidiomycetous yeast T. japonicum in Pseudobatos horkelii in Brazil. This finding highlights the need for further research to determine the potential impact on elasmobranch health, ecology, as well as on commercial fisheries.
survextrap: a package for flexible and transparent survival extrapolation
Authors: Jackson Christopher H. 
Published: November 2023

AbstractBackgroundHealth policy decisions are often informed by estimates of long-term survival based primarily on short-term data. A range of methods are available to include longer-term information, but there has previously been no comprehensive and accessible tool for implementing these.ResultsThis paper introduces a novel model and software package for parametric survival modelling of individual-level, right-censored data, optionally combined with summary survival data on one or more time periods. It could be used to estimate long-term survival based on short-term data from a clinical trial, combined with longer-term disease registry or population data, or elicited judgements. All data sources are represented jointly in a Bayesian model. The hazard is modelled as an M-spline function, which can represent potential changes in the hazard trajectory at any time. Through Bayesian estimation, the model automatically adapts to fit the available data, and acknowledges uncertainty where the data are weak. Therefore long-term estimates are only confident if there are strong long-term data, and inferences do not rely on extrapolating parametric functions learned from short-term data. The effects of treatment or other explanatory variables can be estimated through proportional hazards or with a flexible non-proportional hazards model. Some commonly-used mechanisms for survival can also be assumed: cure models, additive hazards models with known background mortality, and models where the effect of a treatment wanes over time. All of these features are provided for the first time in an R package, , in which models can be fitted using standard R survival modelling syntax. This paper explains the model, and demonstrates the use of the package to fit a range of models to common forms of survival data used in health technology assessments.ConclusionsThis paper has provided a tool that makes comprehensive and principled methods for survival extrapolation easily usable.