bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunolabelling perturbs the endogenous and antibody-conjugated elemental concentrations during immuno-mass spectrometry imaging
Authors: Mello Monique G. , Westerhausen Mika T. , Lockwood Thomas E. , Singh Prashina , Wanagat Jonathan , Bishop David P. 
Published: October 2023

AbstractImmuno-mass spectrometry imaging uses lanthanide-conjugated antibodies to spatially quantify biomolecules via laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The multi-element capabilities allow for highly multiplexed analyses that may include both conjugated antibodies and endogenous metals to reveal relationships between disease and chemical composition. Sample handling is known to perturb the composition of the endogenous elements, but there has been little investigation into the effects of immunolabelling and coverslipping. Here, we used cryofixed muscle sections to examine the impact of immunolabelling steps on the concentrations of a Gd-conjugated anti-dystrophin primary antibody, and the endogenous metals Cu and Zn. Primary antibody incubation resulted in a decrease in Zn, and an increase in Cu. Zn was removed from the cytoplasm where it was hypothesised to be more labile, whereas concentrated locations of Zn remained in the cell membrane in all samples that underwent the immunostaining process. Cu increased in concentration and was found mostly in the cell membrane. The concentration of the Gd-conjugated antibody when compared to the standard air-dried sample was not significantly different when coverslipped using an organic mounting medium, whereas use of an aqueous mounting medium significantly reduced the concentration of Gd. These results build on the knowledge of how certain sample handling techniques change elemental concentrations and distributions in tissue sections. Immunolabelling steps impact the concentration of endogenous elements, and separate histological sections are required for the quantitative analysis of endogenous elements and biomolecules. Additionally, coverslipping tissue sections for complementary immunohistochemical/immunofluorescent imaging may compromise the integrity of the elemental label, and organic mounting media are recommended over aqueous mounting media.
Graphical Abstract
Toward Online Mobile Facility Location on General Metrics
Authors: Ghodselahi Abdolhamid , Kuhn Fabian 
Published: October 2023

AbstractWe introduce an online variant of mobile facility location (MFL) (introduced by Demaine et al. (SODA 258–267 2007)). We call this new problem online mobile facility location (OMFL). In the OMFL problem, initially, we are given a set of k mobile facilities with their starting locations. One by one, requests are added. After each request arrives, one can make some changes to the facility locations before the subsequent request arrives. Each request is always assigned to the nearest facility. The cost of this assignment is the distance from the request to the facility. The objective is to minimize the total cost, which consists of the relocation cost of facilities and the distance cost of requests to their nearest facilities. We provide a lower bound for the OMFL problem that even holds on uniform metrics. A natural approach to solve the OMFL problem for general metric spaces is to utilize hierarchically well-separated trees (HSTs) and directly solve the OMFL problem on HSTs. In this paper, we provide the first step in this direction by solving a generalized variant of the OMFL problem on uniform metrics that we call G-OMFL. We devise a simple deterministic online algorithm and provide a tight analysis for the algorithm. The second step remains an open question. Inspired by the k-server problem, we introduce a new variant of the OMFL problem that focuses solely on minimizing movement cost. We refer to this variant as M-OMFL. Additionally, we provide a lower bound for M-OMFL that is applicable even on uniform metrics.
Comparison of endoscopic versus CT assessment of stone-free status after percutaneous nephrolithotomy (PCNL)
Authors: Hartung F. O. , Müller K. J. , Herrmann J. , Grüne B. , Michel M. S. , Rassweiler-Seyfried M. C. 
Published: October 2023

AbstractThis study is aimed to determine whether postoperative low dose computed tomography (LDCT) imaging is necessary after percutaneous nephrolithotomy (PCNL), or the surgeon's intraoperative assessment of residual fragments (RF) is sufficient and avoidance of postoperative imaging with reduction of radiation exposure can be achieved. Data of all 610 patients who underwent PCNL in prone position in our institution from February 2009 to September 2020 was collected. Parameters such as age, gender, BMI, ASA-Classification, stone related parameters and the surgeon’s assessment of stone-free status were analyzed. The LDCT performed postoperatively was compared to the intraoperative assessment of the surgeon regarding RF. The mean age of patients was 52.82 years; the mean BMI was 28.18 kg/m2. In 418 cases, the surgeon made a clear statement about the presence of RF and postoperative LDCT was carried out. The discrepancy between the two methods (surgeon´s assessment vs. LDCT) was significant at p < 0.0001. The sensitivity, specificity, positive and negative predictive value of the surgeon when assessing RF were 24.05%, 99.45%, 98.28% and 50%. Stone free rate (SFR) after primary PCNL was 45.57%. The overall SFR at discharge was 96.23%. Although the surgeon´s assessment of RF was reliable, postoperative LDCT imaging should still be performed if endoscopic stone clearance is suspected due to the high false negative rate and the low negative predictive value. The optimal timing of postoperative imaging following PCNL remains unclear.
Over ninety years old: Does high cognitive reserve still help brain efficiency?
Authors: Carta Elisa , Riccardi Alice , Marinetto Silvia , Mattivi Sara , Selini Enrico , Pucci Veronica , Mondini Sara 
Published: October 2023

AbstractNonagenarians and centenarians, also called oldest-old, are a very heterogeneous population that counts a limited number of individuals as it is a real challenge to reach this goal. Even if it is well known that cognitive reserve can be considered a factor in maintaining good cognitive functioning in ageing, only very few studies have been carried out on the role of cognitive reserve (CR) in the oldest-old people. The aim of this study is to investigate the relationship between cognitive reserve and cognitive functioning in a population living in a specific region of Italy, the Blue Zone in Sardinia. This population is characterised by extreme longevity and distinctive historical, geographic, social, linguistic and nutritional features. The cognitive Reserve Index questionnaire (CRIq) and the short cognitive Esame Neuropsicologico Breve-2 (ENB-2, Brief Neuropsychological Examination) were administered to 67 participants, all aged between 90 and 105 years old. The CRIq was a predictor of neuropsychological performance for the global score of the battery of tests, ENB-2 (ENB-tot) and also for 7 out of 16 of its sub-tests. All except one (Token) tapped executive functions (Interference memory at 10 and 30 s, TMT-B, Overlapping figures, Abstraction, Fluency). Results highlight that also in the oldest-old population CR has a positive effect on cognition, especially on executive functioning.
Touch imprint cytology is useful for the intraoperative pathological diagnosis of PitNETs’ surgical margins
Authors: Tanabe Noriaki , Inoshita Naoko , Ishida Atsushi , Kato Masataka , Yoshimoto Haruko , Shiramizu Hideki , Suga Hidetaka , Tateno Toru , Ohashi Kenichi , Yamada Shozo 
Published: October 2023

AbstractTouch imprint cytology (TIC) and frozen section (FS) procedures are essential for intraoperative pathological diagnosis (IPD). They are invaluable tools for therapeutic decision-making, helping surgeons avoid under or overtreatment of patients. Pituitary neuroendocrine tumors (PitNETs) are generally small, slow-growing tumors with low-grade malignancy located at the base of the skull where it is impossible to maintain a wide tumor margin. Therefore, transsphenoidal surgery (TSS) should be performed with necessary caution, and with sufficient and minimal resection. Thus, this study aimed to evaluate the diagnostic accuracy of TIC for the diagnosis of PitNET and determine its ability to accurately evaluate the surgical margin compared to the FS procedure. A total of 104 fresh specimens from 28 patients who underwent TSS for PitNETs were examined using TIC and FS. TIC specimens were categorized according to the cell imprinting pattern. All specimens with a large number of neuroendocrine cells diffusely attached to the glass surfaces had PitNET components. Contrarily, no rich or diffuse cell attachments were observed in any non-tumoral endocrine cells. In conclusion, recognizing a pattern of endocrine cell adherence to glass is highly effective in IPD to certify the existence of a PitNET component.
SDN as a defence mechanism: a comprehensive survey
Authors: Ayodele Believe , Buttigieg Victor 
Published: October 2023

AbstractInvesting in cybersecurity is increasingly considered a significant area and aspect a business or organisation should seriously consider. Some of these security solutions are network-based and provide many levels of protection. However, traditional networks are seen to be vendor-specific and are limited, enabling minor to no network flexibility or customisation. Implementing SDN to combat cyberattacks is a workable option for resolving this traditional network constraint. Less attention has been paid to how SDN has been utilised to address security concerns, with most surveys concentrating on the security challenges the SDN paradigm faces. This study aims to provide a comprehensive overview of the state-of-the-art on how SDN has been used to combat attacks between 2017 and 2022 by highlighting the specifics of each literature, its advantages, limitations, and potential areas for further study. This work introduces a taxonomy highlighting SDN’s fundamental traits and contributions as a defence mechanism (SaaDM).
An AI-based English education platform during the COVID-19 pandemic
Authors: Um Hansuk , Kim Hisam , Choi Dain , Oh Hyungna 
Published: October 2023

AbstractThis study examines whether the use of AI-Pengtalk, an AI-based Conversational English programme, provided by a broadcasting company (EBS) that specializes in public education can significantly improve conversational English skills and bridge the English language proficiency gap associated with parental socioeconomic status. Over the course of four weeks from April 27 to May 22 in 2020, 108 fourth-grade classes in 54 elementary schools voluntarily participated in this experiment. Two classes in each school were designated as a treatment group and a control group. For the treatment group, a tablet installed with a pilot version of AI-Pengtalk was provided and students were encouraged to make use of the programme. Two sets of surveys and English tests were placed pre and post hoc. After 4 weeks, test scores, log files, and survey responses of participants were analysed. A series of DID analyses demonstrate that the use of AI-Pengtalk improves the treatment group’s self-evaluation of their English abilities, confidence in using English, preference on English itself, and amount of time spent on studying English during the pilot experimental period compared to the control group. When other variables were controlled, the use of AI-Pengtalk also helped the treatment group achieve higher test scores. This study implicates that the use of smart English education like AI-Pengtalk may especially be able to better compensate for academic setbacks caused by low parental SES or, in the case of English learning, the reluctance to converse in English with other students.
Multiple factors driving the acquisition efficiency of apple proliferation phytoplasma in Cacopsylla melanoneura
Authors: Corretto Erika , Trenti Massimiliano , Štarhová Serbina Liliya , Howie James Malcolm , Dittmer Jessica , Kerschbamer Christine , Candian Valentina , Tedeschi Rosemarie , Janik Katrin , Schuler Hannes 
Published: October 2023

AbstractPhytoplasmas are bacterial pathogens located in the plant’s phloem that are responsible for several plant diseases and are mainly transmitted by phloem-sucking insects. Apple proliferation (AP) is an economically important disease associated with the presence of ‘Candidatus Phytoplasma mali’ which is transmitted by two psyllid species. While Cacopsylla picta is a vector in different regions, the vector efficiency of C. melanoneura varies between different populations. This species is considered the main AP vector in Northwestern Italy but plays a minor role in Northeastern Italy and other European regions. To investigate whether the psyllid and/or the phytoplasma subtype drive the phytoplasma acquisition in C. melanoneura, a phytoplasma acquisition experiment was set up using single mating couples of overwintered individuals from different psyllid populations and phytoplasma subtypes. All analyzed insect populations acquired phytoplasma, but with different efficiencies and concentrations. The main factors driving the acquisition were the phytoplasma subtype and its concentration in the leaves of the infected trees together with the psyllid lineage. The phytoplasma concentration in the psyllids was again influenced by the phytoplasma subtype, the psyllid lineage and the region of origin, whereas the phytoplasma concentration in the leaves and the psyllid haplotype defined with the cytochrome oxidase I gene had only a minor impact on the phytoplasma concentration. This is the first study evaluating the roles of both the psyllid haplotype and the phytoplasma subtype on the acquisition process and highlights the importance of C. melanoneura as an additional AP vector.
Hemodynamic implications of mitral annular calcification in patients undergoing transcatheter aortic valve implantation for severe aortic stenosis
Authors: Hirasawa Kensuke , Butcher Steele C. , Pereira Ana Rita , Meucci Maria Chiara , Stassen Jan , van Rosendael Philippe , Marsan Nina Ajmone , Bax Jeroen J , Delgado Victoria 
Published: October 2023

Abstract
Purposes
Predicting hemodynamic changes of stenotic mitral valve (MV) lesions with mitral annular calcification (MAC) following transcatheter aortic valve implantation (TAVI) may inform clinical decision-making. This study aimed to investigate the association between the MAC severity quantified by computed tomography (CT) and changes in mean transmitral gradient (mTMG), mitral valve area (MVA) and stroke volume index (SVi) following TAVI.

Methods and results
A total of 708 patients (median age 81, 52% male) with severe aortic stenosis (AS) underwent pre-procedural CT and pre- and post-TAVI transthoracic echocardiography. According to the classification of MAC severity determined by CT, 299 (42.2%) patients had no MAC, 229 (32.3%) mild MAC, 102 (14.4%) moderate MAC, and 78 (11.0%) severe MAC. After adjusting for age and sex, there was no significant change in mTMG following TAVI (Δ mTMG = 0.07 mmHg, 95% CI -0.10 to 0.23, P = 0.92) for patients with no MAC. In contrast, patients with mild MAC (Δ mTMG = 0.21 mmHg, 95% CI 0.01 to 0.40, P = 0.018), moderate MAC (Δ mTMG = 0.31 mmHg, 95% CI 0.02 to 0.60, P = 0.019) and severe MAC (Δ mTMG = 0.43 mmHg, 95% CI 0.10 to 0.76, P = 0.0012) had significant increases in mTMG following TAVI, with greater changes associated with increasing MAC severity. In contrast, there was no significant change in MVA or SVi following TAVI.

Conclusion
In patients with severe AS undergoing TAVI, MAC severity was associated with greater increases in post-procedural mTMG whereas MVA or SVi remained unchanged. MAC severity should be considered for potential subsequent MV interventions if TAVI does not improve symptoms.
From Meaning to Ecocide: The Value of Phenomenology for Green Criminology
Authors: Burns Reece 
Published: October 2023

AbstractThe planetary crisis that we face today is not only a result of human-induced environmental degradation, but also of a deep crisis of meaning and value in human existence. In consequence, this article will demonstrate the value of phenomenology towards the existential paradigm within green criminology and its importance to overcome a lived experience that is opposed to the planet’s ecological balance. The article will present Martin Heidegger’s phenomenology through his conception of ‘being-here’, which elaborates on the modes of unreflective and unselfconsciousness of everyday existence. This will then be developed into the theory of being-towards-ecocide that is concerned with the meaning of the individual’s encounter with ecocide. Finally, the value of phenomenology as a conceptual tool for the analysis of green crimes and harms will be outlined as a necessary shift towards transcending ecocide and for an existential, theoretical, and systematic construction for the world of everyday life.
Thread-modular counter abstraction: automated safety and termination proofs of parameterized software by reduction to sequential program verification
Authors: Pani Thomas , Weissenbacher Georg , Zuleger Florian 
Published: October 2023

AbstractParameterized programs are composed of an arbitrary number of concurrent, infinite-state threads. Automated safety and liveness proofs of such parameterized software are hard; state-of-the-art methods for their formal verification rely on intricate abstractions and complicated proof techniques that impede automation. In this paper, we introduce thread-modular counter abstraction (TMCA), a lean new abstraction technique to replace the existing heavy proof machinery. TMCA is a structured abstraction framework built from a novel combination of counter abstraction, thread-modular reasoning, and predicate abstraction. Its major strength lies in reducing the parameterized verification problem to the sequential setting, for which powerful proof procedures, efficient heuristics, and effective automated tools have been developed over the past decades. In this work, we first introduce the TMCA abstraction paradigm, then present a fully automated method for parameterized safety proofs, and finally discuss its application to automated termination and liveness proofs of parameterized software.
High-fat diet, but not duration of lactation, increases mammary gland lymphatic vessel function and subsequent growth of inflammatory breast cancer cells
Authors: Balema Wintana , Morton Janelle , Larson Richard A. , Li Li , Velasquez Fred Christian , Fowlkes Natalie W. , Krishnamurthy Savitri , Debeb Bisrat G. , Sevick-Muraca Eva , Woodward Wendy A. 
Published: October 2023

AbstractInflammatory breast cancer (IBC) presents as rapid-onset swelling and breast skin changes caused by tumor emboli in the breast and breast skin lymphatics. IBC has been linked with obesity and duration of breastfeeding, but how these factors affect IBC tumor progression is not clear. We modeled the simultaneous effects of diet and weaning in mice on in vivo lymphatic function; on IBC tumor growth; and on aspects of the mammary gland microenvironment before and after IBC (SUM149) xenograft inoculation. We hypothesized that weaning status and diet would have synergistic effects on lymphatic function and the breast microenvironment to enhance IBC tumor growth. Changes in lymphatic structure and function were characterized with in vivo near-infrared fluorescence (NIRF) imaging. Mice were fed either a high-fat diet (HFD; 60 kcal%) or a normal/low-fat diet (LFD; 10 kcal%), bred twice, and subjected to either normal-duration nursing (NW) or forced weaning (FW). SUM149 IBC tumors were implanted at 14 months; images were obtained before and after implantation. Multiparous mice fed HFD showed increased pre-tumor lymphatic pulsing in both the FW and NW groups relative to mice fed LFD. HFD promoted tumor growth independent of weaning time (P = 0.04). Pre-tumor lymphatic pulsing was associated with tumor volume at 8 weeks (P = 0.02) and was significantly correlated with expression of the lymphatic tracking ligand CCL21 (P = 0.05, Table 1). HFD significantly increased the numbers of monocyte-derived IBA1+, CD163+, and CD11c+ cells (P < 0.0001, P < 0.0001, P = 0.0005) in the contralateral, non-tumor-bearing mammary gland. Numbers of lymphangiogenic podoplanin+/IBA1+ macrophages were increased in the ducts of HFD and FW mice (all P < 0.003). HFD in nulliparous mice had a similar increase in lymphatic pulsing at 14 weeks (P = 0.006), indicating that this functional change was independent of parity. We conclude that HFD induced increases in mammary gland lymphatic function, assessed as pulsing rate before tumor initiation, and correlated with inflammation in the mammary gland and increased SUM149 tumor growth. The relationship between diet, lymphatic pulsing, and tumor growth warrants further investigation.
Time-series analysis of radon monitoring in soil gas in association with earthquakes in Stivos faulting, at Lagadas basin, North Greece
Authors: Stylianos Stoulos , Alexandra Ioannidou 
Published: October 2023

AbstractTime series analysis was applied to the continuous radon level, temperature, pressure, and rainfall to find clear earthquake signals. Radon signals appeared a few days after heavy rains, and radon signals associated with events M = 3.8–4.2 were detected 12 up to 36 days before. The events are complete data recorded from 1983 to 1986, giving discussion and conclusion on M with prediction time and radon anomaly detected in the Stivos faulting near Thessaloniki, N. Greece.
Has language as resource been the basis for mother-tongue instruction in Sweden? On the evolution of policy orientations towards a uniquely enduring bilingual policy
Authors: Aktürk-Drake Memet 
Published: October 2023

AbstractThis paper provides a comprehensive analysis of the motivations that key policy documents have put forward as justifications for Sweden’s mother-tongue instruction in immigrant and historical minority languages as a multicultural policy that has endured for nearly half a century. The diachronic development of these motivations is analysed in four periods and interpreted with the help of Ruiz’s (1984) orientations in language planning. The corpus consists of 26 key policy documents making up the coordinative discourse among policy actors. Based on an innovative mix of qualitative and quantitative methods, the motivations are presented in a three-tiered taxonomy consisting of motivational units, themes and language-planning orientations. The results point to both continuity and change in how mother-tongue instruction has been justified over time. Confirming previous research, the results show that the language-as-resource orientation has played a central role in justifying both the establishment and the maintenance of mother-tongue instruction in Sweden and that language as right complemented this orientation. Furthermore, the study illustrates that the language-as-problem orientation need not always be detrimental to bilingualism and minority-language maintenance. Contrary to some claims in the literature, it is argued that language as extrinsic resource is not necessarily underpinned by neoliberalism, as there are also social liberal and conservative inroads to this orientation. The paper concludes that although the language-as-resource orientation plays an indispensable role in supporting bilingualism in education, not only the language-as-right orientation but also the language-as-problem orientation should not be neglected.