bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remodelling of cAMP dynamics within the SERCA2a microdomain in heart failure with preserved ejection fraction caused by obesity and type 2 diabetes
Authors: Lai Ping , Hille Susanne S , Subramanian Hariharan , Wiegmann Robert , Roser Pia , Müller Oliver J , Nikolaev Viacheslav O , De Jong Kirstie A 
Published: December 2023

Abstract

Aims
Despite massive efforts, we remain far behind in our attempts to identify effective therapies to treat heart failure with preserved ejection fraction (HFpEF). Diastolic function is critically regulated by sarcoplasmic/endoplasmic reticulum (SR) calcium ATPase 2a (SERCA2a), which forms a functional cardiomyocyte (CM) microdomain where 3′,5′-cyclic adenosine monophosphate (cAMP) produced upon β-adrenergic receptor (β-AR) stimulation leads to phospholamban (PLN) phosphorylation and facilitated Ca2+ re-uptake.

Methods and results
To visualize real-time cAMP dynamics in the direct vicinity of SERCA2a in healthy and diseased myocytes, we generated a novel mouse model on the leprdb background that stably expresses the Epac1-PLN Förster resonance energy transfer biosensor. Mice homozygous for the leprdb mutation (db/db) developed obesity and type 2 diabetes and presented with a HFpEF phenotype, evident by mild left ventricular hypertrophy and elevated left atria filling pressures. Live cell imaging uncovered a substantial β2-AR subtype stimulated cAMP response within the PLN/SERCA2a microdomain of db/db but not healthy control (db/+) CMs, which was accompanied by increased PLN phosphorylation and accelerated calcium re-uptake. Importantly, db/db CMs also exhibited a desensitization of β1-AR stimulated cAMP pools within the PLN/SERCA2a microdomain, which was accompanied by a blunted lusitropic effect, suggesting that the increased β2-AR control is an intrinsic compensatory mechanism to maintain PLN/SERCA2a-mediated calcium dynamics and cardiac relaxation. Mechanistically, this was due to a local loss of cAMP-degrading phosphodiesterase 4 associated specifically with the PLN/SERCA2a complex.

Conclusion
These newly identified alterations of cAMP dynamics at the subcellular level in HFpEF should provide mechanistic understanding of microdomain remodelling and pave the way towards new therapies.
Remnant cholesterol and risk of premature mortality: an analysis from a nationwide prospective cohort study
Authors: Li Likang , Lai Jun , Zhang Jingyi , Van Spall Harriette G C , Thabane Lehana , Lip Gregory Y H , Li Guowei 
Published: December 2023

Abstract

Aims
To explore the relationship between remnant cholesterol (RC) and the risk of premature mortality as well as life expectancy in the general population.

Methods
We included a total of 428 804 participants from the UK Biobank for analyses. Equivalent population percentiles approach based on the low-density lipoprotein cholesterol cut-off points was performed to categorize participants into three RC groups: low (with a mean RC of 0.34 mmol/L), moderate (0.53 mmol/L), and high (1.02 mmol/L). We used multivariable Cox proportional hazards models to evaluate the relationship between RC groups and the risk of premature mortality (defined as death before age 75 years). Life table methods were used to estimate life expectancy by RC groups.

Results
During a median follow-up of 12.1 years (Q1–Q3 11.0–13.0), there were 23 693 all-cause premature deaths documented, with an incidence of 4.83 events per 1000 person-years [95% confidence interval (CI): 4.77–4.89]. Compared with the low RC group, the moderate RC group was associated with a 9% increased risk of all-cause premature mortality [hazard ratio (HR) = 1.09, 95% CI: 1.05–1.14], while the high RC group had an 11% higher risk (HR = 1.11, 95% CI: 1.07–1.16). At the age of 50 years, high RC group was associated with an average 2.2 lower years of life expectancy for females, and an average 0.1 lower years of life expectancy for males when compared with their counterparts in the low RC group.

Conclusions
Elevated RC was significantly related to an increased risk of premature mortality and a reduced life expectancy. Premature death in the general population would benefit from measurement to aid risk stratification and proactive management of RC to improve cardiovascular risk prevention efforts.
Ablation for atrial fibrillation improves the outcomes in patients with heart failure with preserved ejection fraction
Authors: Xie Zhonglei , Qi Baozhen , Wang Zimu , Li Fuhai , Chen Chaofeng , Li Chaofu , Yuan Shuai , Yao Shun , Zhou Jingmin , Ge Junbo 
Published: December 2023

Abstract

Aims
Patients with heart failure with preserved ejection fraction (HFpEF) and atrial fibrillation (AF) have worse clinical outcomes than those with sinus rhythm (SR). We aim to investigate whether maintaining SR in patients with HFpEF through a strategy such as AF ablation would improve outcomes.

Methods and results
This is a cohort study that analysed 1034 patients (median age 69 [63–76] years, 46.2% [478/1034] female) with HFpEF and AF. Of these, 392 patients who underwent first-time AF ablation were assigned to the ablation group, and the remaining 642 patients, who received only medical therapy, were assigned to the no ablation group. The primary endpoint was a composite of all-cause death or rehospitalization for worsening heart failure. After a median follow-up of 39 months, the cumulative incidence of the primary endpoint was significantly lower in the ablation group compared to the no ablation group (adjusted hazard ratio [HR], 0.55 [95% CI, 0.37–0.82], P = 0.003) in the propensity score-matched model. Secondary endpoint analysis showed that the benefit of AF ablation was mainly driven by a reduction in rehospitalization for worsening heart failure (adjusted HR, 0.52 [95% CI, 0.34–0.80], P = 0.003). Patients in the ablation group showed a 33% relative decrease in atrial tachycardia/AF recurrence compared to the no ablation group (adjusted HR, 0.67 [95% CI, 0.54–0.84], P &lt; 0.001).

Conclusion
Among patients with HFpEF and AF, the strategy of AF ablation to maintain SR was associated with a lower risk of the composite outcome of all-cause death or rehospitalization for worsening heart failure.
Lakes-scale pattern of eukaryotic phytoplankton diversity and assembly process shaped by electrical conductivity in central Qinghai-Tibet Plateau
Authors: Zhu Huan , Xiong Xiong , Liu Benwen , Liu Guoxiang 
Published: December 2023

Abstract
Phytoplankton are the main primary producers in aquatic ecosystems and play an important role in food web and geochemical cycles. Its diversity, community structure, and assembly process are influenced by several factors. Alpine lake ecosystems are relatively weak and extremely sensitive to global climate change. However, the impact of climate change on phytoplankton in Qinghai-Tibet Plateau lakes and their responses are still unclear. In this study, we analyzed the diversity, environmental drivers, and assembly process of phytoplankton community in the central QTP lakes. The phytoplankton of these lakes can be primarily distinguished into freshwater and brackish types, with significant differences in species diversity and community dissimilarity. Both shared nearly same key environmental factors that significantly affecting phytoplankton such as EC, and brackish lakes were also positively correlative with TN. Stochastic process was predominant in phytoplankton assembly. Additionally, freshwater and brackish lakes were dominated by dispersal limitation and heterogeneous selection respectively. Alpine lakes had significant EC thresholds, and their diversity and assembly processes changed significantly around the thresholds. The present findings have important implications for understanding and predicting the response of lake phytoplankton communities to climate change and for making decisions to protect the ecological resources of alpine lakes.
Functional guilds and drivers of diversity in seaweed-associated bacteria
Authors: Khan Tahsin , Song Weizhi , Nappi Jadranka , Marzinelli Ezequiel M , Egan Suhelen , Thomas Torsten 
Published: December 2023

Abstract
Comparisons of functional and taxonomic profiles from bacterial communities in different habitats have suggested the existence of functional guilds composed of taxonomically or phylogenetically distinct members. Such guild membership is, however, rarely defined and the factors that drive functional diversity in bacteria remain poorly understood. We used seaweed-associated bacteria as a model to shed light on these important aspects of community ecology. Using a large dataset of over 1300 metagenome-assembled genomes from 13 seaweed species we found substantial overlap in the functionality of bacteria coming from distinct taxa, thus supporting the existence of functional guilds. This functional equivalence between different taxa was particularly pronounced when only functions involved in carbohydrate degradation were considered. We further found that bacterial taxonomy is the dominant driver of functional differences between bacteria and that seaweed species or seaweed type (i.e. brown, red and green) had relatively stronger impacts on genome functionality for carbohydrate-degradation functions when compared to all other cellular functions. This study provides new insight into the factors underpinning the functional diversity of bacteria and contributes to our understanding how community function is generated from individual members.
Genomes of nine biofilm-forming filamentous strains of Cyanobacteria (genera Jaaginema, Scytonema, and Karukerafilum gen. nov.) isolated from mangrove habitats of Guadeloupe (Lesser Antilles)
Authors: Halary Sébastien , Duval Charlotte , Marie Benjamin , Bernard Cécile , Piquet Bérénice , Gros Olivier , Bourguet-Kondracki Marie-Lise , Duperron Sébastien 
Published: December 2023

Abstract
Biofilm-forming cyanobacteria are abundant in mangrove ecosystems, colonizing various niches including sediment surface and periphyton where they can cover large areas, yet have received limited attention. Several filamentous isolates were recently isolated from Guadeloupe, illustrating the diversity and novelty present in these biofilms. In this study, nine strains belonging to three novel lineages found abundantly in Guadeloupe biofilms are characterized by genome sequencing, morphological and ultrastructural examination, metabolome fingerprinting and searched for secondary metabolites biosynthesis pathways. Assignation of two lineages to known genera is confirmed, namely Scytonema and Jaaginema. The third lineage corresponds to a new Coleofasciculales genus herein described as Karukerafilum gen. nov. The four strains belonging to this genus group into two subclades, one of which displays genes necessary for nitrogen fixation as well as the complete pathway for geosmin production. This study gives new insights into the diversity of mangrove biofilm-forming cyanobacteria, including genome-based description of a new genus and the first genome sequence available for the genus Jaaginema.
Under the influence: system-level effects of alcohol industry-funded health information organizations
Authors: Maani Nason , CI van Schalkwyk May , Petticrew Mark 
Published: December 2023

Abstract
There is now an established body of evidence that the alcohol industry seeks to obstruct public health policies that could affect the availability, affordability or marketing of alcohol. In parallel, the alcohol industry is active in funding corporate social responsibility initiatives, with a particular focus on ‘responsible drinking’ campaigns, often facilitated by national-level charities established and/or funded by the alcohol industry and associated organizations. While evidence continues to grow regarding biases in the content produced by such health information organizations, they remain active in partnerships with government health departments on national health promotion campaigns and provide a range of health-related information to the public, community organizations and schools. To understand the implications of such access for policymakers, researchers and the public, there is a need to consider the wider, system-level influences of such organizations and their place in wider alcohol industry strategies. In this article, we describe evolving evidence of the direct and indirect strategic effects of such organizations and demonstrate how they serve key roles in the alcohol industry through their existence, content, partnerships and public profiles. We end by considering the implications for how we conceptualize charities established or funded (entirely or partly) by harmful commodity industries, and to what extent current conflicts of interest guidelines are sufficiently effective.
Immunogenicity and Safety Following 1 Dose of AS01E-Adjuvanted Respiratory Syncytial Virus Prefusion F Protein Vaccine in Older Adults: A Phase 3 Trial
Authors: Schwarz Tino F , Hwang Shinn-Jang , Ylisastigui Pedro , Liu Chiu-Shong , Takazawa Kenji , Yono Makoto , Ervin John E , Andrews Charles P , Fogarty Charles , Eckermann Tamara , Collete Delphine , de Heusch Magali , De Schrevel Nathalie , Salaun Bruno , Lambert Axel , Maréchal Céline , Olivier Aurélie , Nakanwagi Phoebe , Lievens Marc , Hulstrøm Veronica 
Published: December 2023

Abstract

Background
The recently approved AS01E-adjuvanted respiratory syncytial virus (RSV) prefusion F protein–based vaccine for older adults (RSVPreF3 OA) demonstrated high efficacy against RSV-related disease in ≥60-year-olds.

Methods
This ongoing phase 3 study in ≥60-year-olds evaluates immune persistence until 3 years after RSVPreF3 OA vaccination. Here, we describe interim results on humoral and cell-mediated immunogenicity, reactogenicity, and safety until 1 year post–dose 1.

Results
In total, 1653 participants were vaccinated. One month post–dose 1, neutralization titers increased 10.5-fold (RSV-A) and 7.8-fold (RSV-B) vs pre–dose 1. Titers then declined to levels 4.4-fold (RSV-A) and 3.5-fold (RSV-B) above pre–dose 1 at month 6 and remained 3.1-fold (RSV-A) and 2.3-fold (RSV-B) above pre–dose 1 levels after 1 year. RSVPreF3-binding immunoglobulin G levels and CD4+ T-cell frequencies showed similar kinetics. Solicited administration-site and systemic adverse events (mostly mild to moderate and transient) were reported by 62.2% and 49.5% of participants. Serious adverse events were reported by 3.9% of participants within 6 months post–dose 1; 1 case was considered vaccine related.

Conclusions
One RSVPreF3 OA dose elicited cell-mediated and RSV-A– and RSV-B–specific humoral immune responses that declined over time but remained above pre–dose 1 levels for at least 1 year. The vaccine was well tolerated with an acceptable safety profile.
Clinical Trials Registration. NCT04732871 (ClinicalTrials.gov).
A preliminary survey reveals that common viruses are found at low titers in a wild population of honey bees (Apis mellifera)
Authors: Dickey Myra , Whilden Mckaela , Ellis Jordan Twombly , Rangel Juliana 
Published: December 2023

Abstract
A major threat to honey bee (Apis mellifera Linnaeus, Hymenoptera: Apidae) health continues to be parasitism by the mite Varroa destructor, which has been linked to high colony losses worldwide. Besides feeding on developing and adult bees, Varroa is also a prolific vector of honey bee-associated viruses. Because they live in unmanaged conditions, wild honey bee colonies are not treated against Varroa, which has enabled the natural selection of more mite-tolerant bees. To date, few studies have explored the prevalence of viruses in unmanaged colonies. The Welder Wildlife Refuge (WWR) in Texas is a unique site to study the viral landscape of unmanaged honey bees in the United States. The goals of this study were to identify and quantify viruses in wild colonies at the WWR, to examine changes in the prevalence of viruses in these colonies over time, and to compare the presence and titers of viruses between wild colonies at the WWR and those from the nearest managed apiary. We collected bees from colonies at the WWR in 2013, 2016, and 2021, and analyzed selected viruses for their presence and titers via quantitative polymerase chain reaction. In 2021, we also sampled bees from the nearest managed apiary for comparison. We found low average virus titers in all wild colonies sampled, and no difference in virus titers between colonies at the WWR and those from the managed apiary. Our study indicates that virus titers in wild colonies at the WWR are similar to those found in nearby colonies, and that these titers fluctuate over time.
Gender diversity at academic conferences—the case of the International Communication Association
Authors: Braun Maike , Heintz Laura , Kruschinski Simon , Trepte Sabine , Scharkow Michael 
Published: December 2023

Abstract
Gender diversity and the lack of women in leadership in academia have been issues of academic interest for decades. However, little is known about gender diversity at academic conferences as an essential aspect of academia. We investigated 86,719 contributions to International Communication Association (ICA) conferences over the past 18 years with regard to female and male authorship and how it changed following the introduction of childcare, during the global pandemic, and under female division leadership. Lastly, we analyzed divisions/interest groups, authors’ gender, and national affiliation. We found that the proportion of female authors is high in all conference years and is representative of ICA membership. We found differences in how women and men are represented across divisions, countries of author affiliation, based on the availability of childcare, and during the global pandemic. We discuss implications at societal, organizational, and individual levels.
Experiences of working from home: umbrella review
Authors: Hall Charlotte E. , Brooks Samantha K. , Mills Freya , Greenberg Neil , Weston Dale 
Published: December 2023

Abstract
Introduction: The concept of “working from home” is extremely topical following the COVID-19 pandemic; therefore, it is unsurprising that there has been an increased interest in collating research related to homeworking. This has been carried out by multiple reviews, all with slightly different research aims and methodologies. Collating the findings from the available reviews is therefore highly beneficial to establish the experience of homeworking to create recommendations for the future of home-based work.
Methods: An umbrella review was carried out. In June 2022, literature searches were conducted across 4 electronic databases. Published reviews of literature that used a systematic process, were focused on working from home populations, and detailed factors that could be related to the personal experience of homeworking (eg, barriers, facilitators, advantages, disadvantages) were included.
Results: A total of 1930 records were screened and 6 review articles were included. Results report on the following sections: working environment (eg, workplace design, space conditions), personal impact (eg, satisfaction, career impact), and health (eg, physical health, well-being) including a total of 19 themes. Mixed findings were apparent for nearly all included themes, highlighting the need to consider individual and contextual circumstances when researching working from home.
Conclusions: This review establishes the importance of retaining flexibility while homeworking for employees, managers, and organizations. Essentially, a one-size-fits-all approach to working from home is impractical as individual circumstances limit application. Eight recommendations for the future of working from home are suggested.
Clinical effectiveness of trigger point acupuncture on chronic neck and shoulder pain (katakori) with work productivity loss in office workers: a randomized clinical trial
Authors: Minakawa Yoichi , Miyazaki Shogo , Waki Hideaki , Akimoto Yoshiko , Itoh Kazunori 
Published: December 2023

Abstract
Objectives: To evaluate whether trigger point acupuncture (TrPA) is beneficial for office workers who have reduced job performance (presenteeism) due to chronic neck and shoulder pain (katakori).
Methods: A 4-week single-center randomized controlled trial was conducted on 20 eligible female office workers with chronic neck and shoulder pain of at least 3-month duration. The control group implemented only workplace-recommended presenteeism measures, whereas the intervention group received TrPA up to 4 times per month in addition to the presenteeism measures recommended by each workplace. The major outcome measure was the relative presenteeism score on the World Health Organization Health and Work Performance (WHO-HPQ). The secondary outcome measures were pain intensity (numerical rating scale), absolute presenteeism (WHO-HPQ), anxiety and depression (Hospital Anxiety and Depression Scale; HADS), catastrophic thoughts related to pain (Pain Catastrophizing Scale; PCS), and sleep (Athens Insomnia Scale; AIS).
Results: All 9 cases in the intervention group and 11 cases in the control group were analyzed. TrPA up to 4 times per month reduced the intensity of neck and shoulder pain by 20% (P &lt; .01, d = 1.65) and improved labor productivity (relative presenteeism value) by 0.25 (P &lt; .01, d = 1.33) compared with the control group over 1 month. No significant differences were observed between the 2 groups in terms of absolute presenteeism score, HADS, PCS, or AIS.
Conclusions:These results suggest that regular intervention with TrPA may be effective in the relative presenteeism score before and after the intervention and the degree of neck and shoulder pain over 28 days compared with the control group.
Can you See? Actuality Entailments in the Present
Authors: Dieuleveut Anouk 
Published: December 2023

Abstract
This paper argues that English present ability modal statements like “I can see Saturn” are ambiguous in the same way as past ability statements like “I was able to lift a fridge”: they can express either a general ability (‘I have the ability to see Saturn, in general’), or have an actualized (episodic) interpretation (‘I'm seeing Saturn, right now’). The challenge is to explain why in the present, actualized interpretations are only licensed when the modal's prejacent is a perception verb like see, and not with other predicates: “I can watch Saturn” only has the general ability reading available, and not the actualized one. I propose that (i) similar to what has been shown for past modal statements in the literature on Actuality Entailments (AEs) (Bhatt 1999), the ambiguity depends on grammatical aspect: general ability readings are due to the imperfective, which “removes” AEs by having the event occur in worlds introduced by a generic operator (Bhatt 1999), and actualized readings are due to the perfective, which directly combines with the prejacent event across the modal (Hacquard 2009); (ii) The usual unavailability of actualized interpretations in the present comes from the Present Perfective Paradox (Malchukov 2009): perfective aspect is incompatible with present tense, because the event time, a time interval, cannot be contained within the punctual speech time. (iii) Perception verbs are special in that they, and only they, are able to combine with perfective in the present, either because the PPP does not arise at all, or because they allow a specific type of aspectual coercion. This also explains their behavior in (non-modal) simple present sentences. A second challenge is that actualized interpretations in the present appear to occur exclusively with ability modals, and not when modals express other root flavors (e.g. teleological or deontic). I propose that this restriction is due to a further temporal orientation constraint.