bluuebunny/crossref_metadata_2025_split huggingface dataset:
The American Thyroid Association risk classification of papillary thyroid cancer according to presurgery cytology
Authors: Croce Laura , Teliti Marsida , Chytiris Spyridon , Sparano Clotilde , Coperchini Francesca , Villani Laura , Calì Benedetto , Petrone Luisa , Magri Flavia , Trimboli Pierpaolo , Rotondi Mario 
Published: February 2024

Abstract

Objective
To compare the American Thyroid Association (ATA) risk staging of histologically proven papillary thyroid cancer (PTC) in patients who received a presurgery cytologic result of either indeterminate thyroid nodules (ITNs, Bethesda III/IV) or suspicious for malignancy/malignant (TIR 4/5, Bethesda V/VI).

Methods
Clinical, ultrasonographic, cytological data from patients with histologically diagnosed PTC were retrospectively collected.

Results
Patients were stratified according to the preoperative fine-needle aspiration cytology into 2 groups: 51 ITNs (TIR3A/3B) and 118 suspicious/malignant (TIR 4/5). Male/female ratio, age, and presurgery TSH level were similar between the 2 groups. At ultrasound, TIR 4/5 nodules were significantly more frequently hypoechoic (P = .037), with irregular margins (P = .041), and with microcalcifications (P = .020) and were more frequently classified as high-risk according to the European Thyroid Imaging and Reporting Data System (EU-TIRADS; P = .021). At histology, the follicular PTC subtype was significantly more prevalent among ITNs while classical PTC subtype was more frequent in TIR 4/5 group (P = .002). In TIR 4/5 group, a higher rate of focal vascular invasion (P &lt; .001) and neck lymph node metastasis (P = .028) was observed. Intermediate-risk category according to ATA was significantly more frequent in TIR 4/5 group while low-risk category was more frequently found among ITNs (P = .021), with a higher number of patients receiving radioiodine in TIR 4/5 group (P = .002). At multivariate logistic regression, having a TIR 4/5 cytology was associated with a significant risk of having a higher ATA risk classification as compared to ITN (OR 4.6 [95% CI 1.523-14.007], P = .007), independently from presurgery findings (nodule size at ultrasound, sex, age, and EU-TIRADS score).

Conclusions
Papillary thyroid cancers recorded among ITNs are likely less aggressive and are generally assessed as at lower risk according to ATA classification.
Welfare state policy and educational inequality: a cross-national multicohort study
Authors: Schoenholzer Kevin , Burger Kaspar 
Published: February 2024

Abstract
Proponents of welfare policy have argued that publicly funded early childhood education and care (ECEC), paid parental leave, and family benefits spending can weaken the influence of social background on educational outcomes by providing a supplementary source of early investment that particularly benefits disadvantaged families. We analyze whether the welfare state context in which children spend their early childhood (ages 0–5) moderates the association between parental educational attainment and the child’s educational achievement at age 10. We combine data from two large-scale international student assessments with data about welfare state policies. Results from multilevel models show that countries with higher public ECEC spending and higher family benefits spending exhibited a weaker association between parental education and student math achievement. Countries with longer parental leave exhibited a stronger association between parental education and student math, science, and reading achievement. Findings provide evidence of the mixed role of welfare state policies for social inequality in student achievement.
Barriers to research in family medicine—interviews with Portuguese family physician researchers
Authors: Gil Conde Margarida , Rodrigues Vera L , Ramos Raquel C , Rente Ana , Broeiro-Gonçalves Paula , Ribeiro Cristina , Nicola Paulo J 
Published: February 2024

Abstract

Background
There is a need for a deeper understanding of the barriers to research in family medicine (FM) and to consider the perceptions and perspectives of professionals. Our study aims to provide a strategic view for research capacity building in FM. We included the perspective of family physician researchers (FPR) on the existing barriers to investigation in this context.

Objectives
To understand and characterize the barriers to research in FM (personal and structural), from the perspective of Portuguese family physicians who are researchers.

Methods
A qualitative study, of phenomenological nature, was performed, through the conduction of semi-structured interviews with FPR, from 2019 to 2022. Data analysis and thematic coding were done on MAxQDA®, with inductive and deductive approaches, until data saturation was reached.

Results
A total of 12 family physicians/researchers were interviewed. Seven main themes were identified as barriers to research: time, professional valorization, funding, ethics committees, infrastructure, management/institutions, and participants. Each theme is divided into subthemes that make it possible to assess how a barrier can affect researchers in performing research activities.

Conclusion
Our study highlights the identification of 7 main barriers. Structuring them into sub-themes not only improved the organization of our results but also provided robust support for the next phase, namely the application of a survey with the aim of gaining a deeper insight into the repercussions that these barriers to FPR have at a national level. This research is crucial to laying the foundations for a policy document that offers well-defined and tailored recommendations to address the barriers we have uncovered.
Excessive tubulin glutamylation leads to progressive cone-rod dystrophy and loss of outer segment integrity
Authors: Aljammal Rawaa , Saravanan Thamaraiselvi , Guan Tongju , Rhodes Scott , Robichaux Michael A , Ramamurthy Visvanathan 
Published: February 2024

Abstract
Mutations in Cytosolic Carboxypeptidase-like Protein 5 (CCP5) are associated with vision loss in humans. To decipher the mechanisms behind CCP5-associated blindness, we generated a novel mouse model lacking CCP5. In this model, we found that increased tubulin glutamylation led to progressive cone-rod dystrophy, with cones showing a more pronounced and earlier functional loss than rod photoreceptors. The observed functional reduction was not due to cell death, levels, or the mislocalization of major phototransduction proteins. Instead, the increased tubulin glutamylation caused shortened photoreceptor axonemes and the formation of numerous abnormal membranous whorls that disrupted the integrity of photoreceptor outer segments (OS). Ultimately, excessive tubulin glutamylation led to the progressive loss of photoreceptors, affecting cones more severely than rods. Our results highlight the importance of maintaining tubulin glutamylation for normal photoreceptor function. Furthermore, we demonstrate that murine cone photoreceptors are more sensitive to disrupted tubulin glutamylation levels than rods, suggesting an essential role for axoneme in the structural integrity of the cone outer segment. This study provides valuable insights into the mechanisms of photoreceptor diseases linked to excessive tubulin glutamylation.
Identification and characterization of two P450 enzymes from Citrus sinensis involved in TMTT and DMNT biosyntheses and Asian citrus psyllid defense
Authors: Sun Xueli , Hu Chunhua , Yi Ganjun , Zhang Xinxin 
Published: February 2024

Abstract
The homoterpenes (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are the major herbivore-induced plant volatiles that help in defense directly by acting as repellants and indirectly by recruiting insects’ natural enemies. In this study, DMNT and TMTT were confirmed to be emitted from citrus (Citrus sinensis) leaves infested with Asian citrus psyllid (Diaphorina citri Kuwayama; ACP), and two cytochrome P450 (CYP) genes (CsCYP82L1 and CsCYP82L2) were newly identified and characterized. Understanding the functions of these genes in citrus defense will help plan strategies to manage huanglongbing caused by Candidatus Liberibacter asiaticus (CLas) and spread by ACP. Quantitative real-time PCR (qPCR) analysis showed that CsCYP82L1 and CsCYP82L2 were significantly upregulated in citrus leaves after ACP infestation. Yeast recombinant expression and enzyme assays indicated that CsCYP82L1 and CsCYP82L2 convert (E)-nerolidol to DMNT and (E,E)-geranyllinalool to TMTT. However, citrus calluses stably overexpressing CsCYP82L1 generated only DMNT, whereas those overexpressing CsCYP82L2 produced DMNT and TMTT. Furthermore, ACPs preferred wild-type lemon (Citrus limon) over the CsCYP82L1-overexpressing line in dual-choice feeding assays and mineral oil over TMTT or DMNT in behavioral bioassays. Finally, yeast one-hybrid, electrophoretic mobility shift, and dual luciferase assays demonstrated that CsERF017, an AP2/ERF transcription factor, directly bound to the CCGAC motif and activated CsCYP82L1. Moreover, the transient overexpression of CsERF017 in lemon leaves upregulated CsCYP82L1 in the absence and presence of ACP infestation. These results provide novel insights into homoterpene biosynthesis in C. sinensis and demonstrate the effect of homoterpenes on ACP behavior, laying a foundation to genetically manipulate homoterpene biosynthesis for application in huanglongbing and ACP control.
Bacterial infection in endometriosis: a silver-lining for the development of new non-hormonal therapy?
Authors: Khan Khaleque N , de Ziegler Dominique , Guo Sun-Wei 
Published: February 2024

Abstract
The pathogenesis of endometriosis is a hotly debated topic, yet still cloaked in multiple layers of hypothetical theories. A recent report raises the possibility that bacterial infection, especially those of the genus Fusobacterium, may be the cause of endometriosis, at least in certain women. More importantly, the demonstration that treatment with broad-spectrum antibiotics significantly reduced the size of lesions in a mouse endometriosis model rekindles the hope for new non-hormonal treatments. The development of new therapies has been plagued by strings of unsuccessful clinical trials over the last two decades. Is this antibiotic therapy, a silver lining for the research and development of non-hormonal drugs for endometriosis?
Assembling China’s Belt and Road Initiative: Discourse, Institution, and Materials
Authors: Hu Ran 
Published: February 2024

AbstractThe article uses the concept of assemblage to analyze the becoming, not being, of China’s Belt and Road Initiative (BRI), that is, the ways in which different elements were framed and constructed so as to constitute the BRI, often seen as China’s “grand” or “global” strategy. Different from rational and more-than-rational perspectives, the assemblage approach pays attention to the role of materials as well as the contingencies that were associated with the BRI. Thus, the article argues that the global BRI is best conceptualized as an assemblage that emerged through (de)territorializing processes in which various practices, institutions, discourses, and materials came together to form different relations with specific effects, rather than a set of principles simply conceived and imposed by China’s central government. The Chinese governmental institutions and national discourses are critical to its emergence, but so are materials such as the forms of policy papers and infrastructure. Seeing it this way allows us to understand how such a broad and heterogenous strategy as the BRI is held together without ceasing to be heterogenous; in other words, how the BRI emerges, travels, and mutates.
Coral mucus as a reservoir of bacteriophages targeting Vibrio pathogens
Authors: Rubio-Portillo Esther , Robertson Sophia , Antón Josefa 
Published: February 2024

Abstract
The increasing trend in sea surface temperature promotes the spread of Vibrio species, which are known to cause diseases in a wide range of marine organisms. Among these pathogens, Vibrio mediterranei has emerged as a significant threat, leading to bleaching in the coral species Oculina patagonica. Bacteriophages, or phages, are viruses that infect bacteria, thereby regulating microbial communities and playing a crucial role in the coral’s defense against pathogens. However, our understanding of phages that infect V. mediterranei is limited. In this study, we identified two phage species capable of infecting V. mediterranei by utilizing a combination of cultivation and metagenomic approaches. These phages are low-abundance specialists within the coral mucus layer that exhibit rapid proliferation in the presence of their hosts, suggesting a potential role in coral defense. Additionally, one of these phages possesses a conserved domain of a leucine-rich repeat protein, similar to those harbored in the coral genome, that plays a key role in pathogen recognition, hinting at potential coral–phage coevolution. Furthermore, our research suggests that lytic Vibrio infections could trigger prophage induction, which may disseminate genetic elements, including virulence factors, in the coral mucus layer. Overall, our findings underscore the importance of historical coral–phage interactions as a form of coral immunity against invasive Vibrio pathogens.
The Separability Thesis
Authors: Spoor Iris 
Published: February 2024

Abstract
Debates over the metaphysical status of aesthetic properties have persisted for decades in Aesthetics. One question that consistently arises in any discussion of aesthetic properties is whether they are fundamentally evaluative or descriptive in character. Aesthetic properties are often treated as parallel to moral properties which means many philosophers take it for granted that aesthetic properties are fundamentally evaluative. There are some philosophers, like Frank Sibley and Jerrold Levinson, who take the road less traveled and treat aesthetic properties as primarily descriptive in character. In this paper, I outline Levinson’s intriguing view that the evaluative aspect of an aesthetic property can be canceled leaving us with the descriptive essence of the property. I refer to this as the separability thesis. It contends that evaluative reactions to aesthetic properties can be separated from the objective, perceptual content of an aesthetic experience thereby leaving us with the non-evaluative core of the property. I go on to defend the separability thesis from a powerful challenge developed by Rafael De Clercq. Ultimately, I contend that while De Clercq’s argument is clever and insightful it fails to undermine the separability thesis.
In vitro activity of cefiderocol and comparator antibiotics against multidrug-resistant non-fermenting Gram-negative bacilli
Authors: Huang Yu-Shan , Chuang Yu-Chung , Chen Pao-Yu , Chou Pei-Chun , Wang Jann-Tay 
Published: February 2024

Abstract

Background
This study evaluated the in vitro activity of cefiderocol, ceftazidime/avibactam, and aztreonam/avibactam against clinically important multidrug-resistant non-fermenting Gram-negative bacilli.

Methods
Bacteraemic isolates of 126 multidrug-resistant Acinetobacter baumannii (MDRAB), 110 imipenem-resistant Pseudamoas aeruginosa [including 14 difficult-to-treat resistant P. aeruginosa (DTRPA)], 45 beta-lactam-non-susceptible Burkholderia cepacia complex (BCC), 47 levofloxacin or trimethoprim/sulfamethoxazole-non-susceptible Stenotrophomonas maltophilia and 22 ciprofloxacin-non-susceptible Elizabethkingia spp. collected between 2019 and 2021 were subjected to MIC determination for cefiderocol, ceftazidime/avibactam and aztreonam/avibactam.

Results
The MIC50/90s of ceﬁderocol for drug-resistant A. baumannii, P. aeruginosa, BCC, S. maltophilia and Elizabethkingia spp. were 0.25/2, 0.25/1, ≤0.06/≤0.06, ≤0.06/0.25 and &gt;32/&gt;32 mg/L, respectively. Cefiderocol inhibited 94.4% (119/126) of MDRAB, 100% of imipenem-resistant P. aeruginosa, 100% of DTRPA and 100% of BCC at an MIC ≤4 mg/L, and 97.9% (46/47) of S. maltophilia at ≤1 mg/L. Ceftazidime/avibactam inhibited 76.4% (84/110) of imipenem-resistant P. aeruginosa, 21.4% (3/14) of DTRPA and 68.9% (31/45) of BCC at an MIC ≤8 mg/L. Aztreonam/avibactam had MIC50/90s of 16/&gt;32, 8/16 and 4/8 mg/L for imipenem-resistant P. aeruginosa, BCC and S. maltophilia, respectively. At ≤8 mg/L, aztreonam/avibactam inhibited 7.1% (1/14) of DTRPA and 93.6% (44/47) of S. maltophilia isolates. Elizabethkingia spp. demonstrated high MICs for cefiderocol, ceftazidime/avibactam and aztreonam/avibactam, with all MIC50s and MIC90s &gt; 32 mg/L.

Conclusion
Cefiderocol may serve as an alternative treatment for multidrug-resistant A. baumannii, P. aeruginosa, BCC and S. maltophilia when other antibiotics have been ineffective or intolerable. The role of ceftazidime/avibactam and aztreonam/avibactam in the management of BCC or S. maltophilia infections warrants further investigation.
Effect of the initial pH of the culture medium on the nutrient consumption pattern of Bifidobacterium animalis subsp. lactis Bb12 and the improvement of acid resistance by purine and pyrimidine compounds
Authors: Zhang Zongcai , Yin Boxing , Liu Fei , Zhou Wei , Wang Mengrui , Chang Ziqing , Zhou Junping , Yue Mingzhe , Chen Junxia , Feng Zhen 
Published: February 2024

Abstract

Aims
During fermentation, the accumulation of acidic products can induce media acidification, which restrains the growth of Bifidobacterium animalis subsp. lactis Bb12 (Bb12). This study investigated the nutrient consumption patterns of Bb12 under acid stress and effects of specific nutrients on the acid resistance of Bb12.

Methods and results
Bb12 was cultured in chemically defined medium (CDM) at different initial pH values. Nutrient consumption patterns were analyzed in CDM at pH 5.3, 5.7, and 6.7. The patterns varied with pH: Asp + Asn had the highest consumption rate at pH 5.3 and 5.7, while Ala was predominant at pH 6.7. Regardless of the pH levels (5.3, 5.7, or 6.7), ascorbic acid, adenine, and Fe2+ were vitamins, nucleobases, and metal ions with the highest consumption rates, respectively. Nutrients whose consumption rates exceeded 50% were added individually in CDM at pH 5.3, 5.7, and 6.7. It was demonstrated that only some of them could promote the growth of Bb12. Mixed nutrients that could promote the growth of Bb12 were added to three different CDM. In CDM at pH 5.3, 5.7, and 6.7, it was found that the viable cell count of Bb12 was the highest after adding mixed nutrients, which were 8.87, 9.02, and 9.10 log CFU ml−1, respectively.

Conclusions
The findings suggest that the initial pH of the culture medium affects the nutrient consumption patterns of Bb12. Specific nutrients can enhance the growth of Bb12 under acidic conditions and increase its acid resistance.
Human technology intermediation to reduce cognitive load: understanding healthcare staff members’ practices to facilitate telehealth access in a Federally Qualified Health Center patient population
Authors: Williamson Alicia K , Antonio Marcy G , Davis Sage , Kameswaran Vaishnav , Dillahunt Tawanna R , Buis Lorraine R , Veinot Tiffany C 
Published: February 2024

Abstract

Objectives
The aim of this study was to investigate how healthcare staff intermediaries support Federally Qualified Health Center (FQHC) patients’ access to telehealth, how their approaches reflect cognitive load theory (CLT) and determine which approaches FQHC patients find helpful and whether their perceptions suggest cognitive load (CL) reduction.

Materials and Methods
Semistructured interviews with staff (n = 9) and patients (n = 22) at an FQHC in a Midwestern state. First-cycle coding of interview transcripts was performed inductively to identify helping processes and participants’ evaluations of them. Next, these inductive codes were mapped onto deductive codes from CLT.

Results
Staff intermediaries used 4 approaches to support access to, and usage of, video visits and patient portals for FQHC patients: (1) shielding patients from cognitive overload; (2) drawing from long-term memory; (3) supporting the development of schemas; and (4) reducing the extraneous load of negative emotions. These approaches could contribute to CL reduction and each was viewed as helpful to at least some patients. For patients, there were beneficial impacts on learning, emotions, and perceptions about the self and technology. Intermediation also resulted in successful visits despite challenges.

Discussion
Staff intermediaries made telehealth work for FQHC patients, and emotional support was crucial. Without prior training, staff discovered approaches that aligned with CLT and helped patients access technologies. Future healthcare intermediary interventions may benefit from the application of CLT in their design. Staff providing brief explanations about technical problems and solutions might help patients learn about technologies informally over time.

Conclusion
CLT can help with developing intermediary approaches for facilitating telehealth access.
Job search strategies and early careers of clinical informatics fellowship alumni (2016-2022)
Authors: Kim Ellen , Van Cain Melissa , Hron Jonathan D 
Published: February 2024

Abstract

Objective
To report on clinical informatics (CI) fellows’ job search and early careers.

Materials and Methods
In the summer of 2022, we performed a voluntary and anonymous survey of 242 known clinical informatics fellowship alumni from 2016 to 2022. The survey included questions about their initial job search process; first job, salary, and informatics time after training; and early career progression over the first 1-6 years after fellowship.

Results
Nearly half (101, 41.7%) responded to the survey. Median informatics time was 50%; most were compensated similar/better than a purely clinical position. Most reported CI fellowship significantly impacted their career, were satisfied with their first and current job after training, and provided advice for current fellows and CI education leaders. Graduates in 2022 had a median job search of 5 months, beginning 3-15 months before graduation; most had a position created for them. Nearly all graduates from 2016-2021 (61, 93.8%) had at least one change in roles/benefits since finishing training, with a trend for increased informatics time and salary.

Discussion
There was a wide variety of roles, salary, and funding sources for CI positions. This highlights some of the unique challenges CI fellows face and the importance of networking. These results will help CI education leaders, fellows, alumni, and prospective fellowship applicants.

Conclusion
Graduates felt that CI fellowship had a significant impact on their career, were pleased with their first jobs and early career trajectory. Continued follow-up of the experience of new graduates and alumni is needed to assess emerging patterns over time.
An open-source platform for pediatric cancer data exploration: a report from Data for the Common Good
Authors: Wyatt Kirk D , Graglia Luca , Furner Brian , Kang Bobae , Fitzsimons Michael , Grossman Robert L , Volchenboum Samuel L 
Published: February 2024

Abstract

Objective
The Pediatric Cancer Data Commons (PCDC)—a project of Data for the Common Good—houses clinical pediatric oncology data and utilizes the open-source Gen3 platform. To meet the needs of end users, the PCDC development team expanded the out-of-box functionality and developed additional custom features that should be useful to any group developing similar data commons.

Materials and Methods
Modifications of the PCDC data portal software were implemented to facilitate desired functionality.

Results
Newly developed functionality includes updates to authorization methods, expansion of filtering capabilities, and addition of data analysis functions.

Discussion
We describe the process by which custom functionalities were developed. Features are open source and available to be implemented and adapted to suit needs of data portals that utilize the Gen3 platform.

Conclusion
Data portals are indispensable tools for facilitating data sharing. Open-source infrastructure facilitates a modular and collaborative approach for meeting needs of end users and stakeholders.