bluuebunny/crossref_metadata_2025_split huggingface dataset:
Redox Activated Substrates for Enhancing Activatable Cyclopropene Bioorthogonal Reactions
Authors: Kao Wei-Siang , Huang Wei , Zhang Yunlei , Meyer Andrea , Laughlin Scott T. 
Published: October 2023

ABSTRACTBioorthogonal chemistry has become a mainstay in chemical biology and is making inroads in the clinic with recent advances in protein targeting and drug release. Since the field’s beginning, a major focus has been on designing bioorthogonal reagents with good selectivity, reactivity, and stability in complex biological environments. More recently, chemists have imbued reagents with new functionalities like click-and-release or light/enzyme-controllable reactivity. We have previously developed a controllable cyclopropene-based bioorthogonal ligation, which has excellent stability in physiological conditions and can be triggered to react with tetrazines by exposure to enzymes, biologically significant small molecules, or light spanning the visual spectrum. Here, to improve reactivity and gain a better understanding of this system, we screened diene reaction partners for the cyclopropene. We found that a cyclopropene–quinone pair is 26 times faster than reactions with 1,2,4,5-tetrazines. Additionally, we showed that the reaction of the cyclopropene–quinone pair can be activated by two orthogonal mechanisms, caging group removal on the cyclopropene and oxidation/reduction of the quinone. Finally, we demonstrated that this caged cyclopropene–quinone can be used as a bioorthogonal imaging tool to label the membranes of cultured cells.
Reassessing the substrate specificities of the majorStaphylococcus aureuspeptidoglycan hydrolases lysostaphin and LytM
Authors: Antenucci Lina , Virtanen Salla , Thapa Chandan , Jartti Minne , Pitkänen Ilona , Tossavainen Helena , Permi Perttu 
Published: October 2023

AbstractOrchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth and viability. Although the function of several PG synthetases and hydrolases is well-understood, the function, regulation, and mechanism of action of PG hydrolases characterized as lysostaphin-like endopeptidases have remained elusive. Many of these M23 family members can hydrolyse glycyl-glycine peptide bonds and show lytic activity againstStaphylococcus aureuswhose PG contains a pentaglycine bridge, but their exact substrate specificity and hydrolysed bonds are still vaguely determined.In this work, we have employed NMR spectroscopy to study both the substrate specificity and the bond cleavage of the bactericide lysostaphin and theS. aureusPG hydrolase LytM. Yet, we provide substrate-level evidence for the functional role of these enzymes. Indeed, our results show that the substrate specificities of these structurally highly homologous enzymes are similar, but unlike observed earlier both LytM and lysostaphin prefer the D-Ala-Gly cross-linked part of mature peptidoglycan. However, we show that while lysostaphin is genuinely a glycyl-glycine hydrolase, LytM can also act as a D-alanyl-glycine endopeptidase.
Cleave and Rescue gamete killers create conditions for gene drive in plants
Authors: Oberhofer Georg , Johnson Michelle L. , Ivy Tobin , Antoshechkin Igor , Hay Bruce A. 
Published: October 2023

AbstractGene drive elements promote the spread of linked traits, even when their presence confers a fitness cost to carriers, and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs (the Cleaver/Toxin) that disrupts endogenous versions of an essential gene, and a recoded version of the essential gene resistant to cleavage (the Rescue/Antidote).ClvRspreads by creating conditions in which those lackingClvRdie because they lack functional versions of the essential gene. We demonstrate the essential features ofClvRgene drive in the plantArabidopsis thalianathrough killing of gametes that fail to inherit aClvRthat targets the essential gene YKT61, whose expression is required in male and female gametes for their survival. Resistant (uncleavable but functional) alleles, which can slow or prevent drive, were not observed. Modeling shows plantClvRs are likely to be robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications in plant breeding, weed control, and conservation are discussed.
FluxNorm: Toolbox for metabolic flux assay normalization byin situcell counting
Authors: Djaja Nathalie A. , Bracha Teva , Yu Seungyoon B. , Wang Haoming , Carlson Natasha M. , Pekkurnaz Gulcin 
Published: October 2023

ABSTRACTPlate-based quantitative metabolic flux analysis has emerged as the central technology to examine cellular metabolism and mitochondrial bioenergetics. However, accurate interpretation of metabolic activity between different experimental conditions in multi-well microplates requires data normalization based onin situcell counts. Here, we describe FluxNorm, a platform-independent semi-automated computational workflow, validated for three different cell types, to normalize cell density for accurate assessment of cellular bioenergetics.
Threat awareness and counter-terrorism preparedness of Dutch hospitals: a cross-sectional survey
Authors: Barten Dennis G. , Janssen Maud , De Cauwer Harald , Keereweer Dennis , Tan Edward , van Osch Frits , Mortelmans Luc 
Published: October 2023

ABSTRACTBackgroundWorkplace violence, including violent extremism, is a growing concern in the healthcare environment. Furthermore, there has been a disproportionate rise in the rate of terrorist attacks on hospitals during the past two decades. Hospitals are vulnerable targets due to their easy accessibility and their high density of patients, staff and visitors. Nonetheless, little is known about the hospitals’ awareness of these risks, and to which extent these facilities protect themselves from violent extremism and terrorist attacks.MethodsThis was a cross-sectional survey study among emergency managers of acute care hospitals in the Netherlands. The questionnaire included 42 items across six themes: demographic (hospital) data; general and emergency department (ED) access control; ED security and preparedness; online security and offline transparency; violence, terrorism and warfare; and impact of the COVID-19 pandemic. Responses were collected and stored in a secured online database, and subsequently exported to an Excel spreadsheet for descriptive analysis. Continuous data were reported as means or as medians with interquartile ranges (IQR), using SPSS. Categorical data were reported as absolute numbers and as valid percentages.ResultsThe questionnaire was completed on behalf of 33 out of 71 hospital organizations (46%), representing 38 out of 82 EDs (46%). Hospitals had broadly different policies with regards to patient and visitor registration, and the presence of security guards. Most hospitals had controlled vehicle access for the parking lot and ambulance bay, but this was not 24/7 in all hospitals. A paragraph on terrorist attacks was included in 34% of hospital disaster plans. Eighteen percent of hospitals had previous experience with acts of violent extremism and 55% of hospitals had sustained (attempted) cyberattacks. Whilst the likelihood of a physical terrorist attack was deemed low at 3.6 (median 4, IQR 2.6) on a 10-point Likert scale, the likelihood of a cyberattack was considered high at 7.3 (median 8, IQR 2.3). A significant proportion of emergency managers reported to experience an increased risk of violence since the onset of the COVID-19 pandemic.ConclusionPractice variation with regards to counter-terrorism defence measures in Dutch hospitals is high. The preparedness of hospitals for terrorist attacks or acts of violent extremism could be improved and may benefit from uniform, evidence based hospital security policies.
Marketplace accessibility: a service-provider perspective
Authors: Husemann Katharina C. , Zeyen Anica , Higgins Leighanne 
Published: October 2023

Purpose
This study aims to explore the strategies that service providers use to facilitate marketplace accessibility, and identify the key challenges in that process. The authors do so to develop a roadmap towards improved accessibility and disability inclusion in the marketplace.

Design/methodology/approach
The authors conducted eight semi-structured interviews with service providers (curators, visitor service coordinators and access managers) at museums who run access programmes for customers with visual impairment (VI), along with an embodied duo-ethnography of those programmes.

Findings
Service providers foster autonomous, embodied and social access. Resource constraints, safety concerns and exposed differences between customers compromise access. To overcome these challenges, service providers engage in three inclusionary strategies – informing, extending and sensitizing.

Research limitations/implications
This service provider- and VI-focus present limitations. Future research should consider a poly-vocal approach that includes the experiences of numerous stakeholders to holistically advance marketplace accessibility; and apply the marketplace accessibility findings upon different disabilities in other marketplace contexts.

Practical implications
This study offers a roadmap for policymakers and service providers on: which types of access should and can be created; what challenges may be encountered; how to manage these challenges; and, thus, how to advance accessibility beyond regulations.

Originality/value
This study contributes a service provider perspective on marketplace accessibility that goes beyond removing “disabling” barriers towards creating opportunities for co-creation; an approach towards marketplace accessibility that fosters inclusiveness while considering the inherent challenges of that process; and an illustration of posthumanism’s empirical value in addressing issues of accessibility in the marketplace.
The determinants of abusive supervision
Authors: Moin Muhammad Farrukh , Khan Ali Nawaz 
Published: October 2023

Purpose
On the basis of affective events theory, this study aims to examine the connection between work-related events (i.e. supervisor role ambiguity and role conflicts) and abusive supervision via emotion (i.e. supervisor frustration). This study also examines the moderating role of supervisor personality traits (i.e. neuroticism and conscientiousness).

Design/methodology/approach
This study collected lagged and multisource field data (472 subordinates and supervisors dyads) from the service organizations.

Findings
The data collected supported majority of this study’s hypothesized relationships regarding determinants of abusive supervision.

Practical implications
This study underlines what triggers supervisor abuse. This study also enables organizations with the intervention opportunity to reduce the effects of supervisor role ambiguity, role conflict, negative emotions and personality on triggering abusive supervision.

Originality/value
Prior research on abusive supervision has extensively focused on its outcomes, leaving a noteworthy research gap about what triggers abusive supervision. To fill this important gap in leadership literature, this study proposed and tested a research model of determinants of supervisor abuse. Thus, this study contributes to leadership and abusive supervision research. Implications and future research directions are discussed.
Shareholder activism and accounting reporting complexity
Authors: Dao Mai , Xu Hongkang 
Published: October 2023

PurposeIn this paper the authors aim to examine whether shareholder activism is associated with accounting reporting complexity (ARC).Design/methodology/approachThe authors employ ordinary least squares (OLS) and a sample of 19,530 firm-year observations (representing 3,377 unique firms) over the 2010–2019 period to test the prediction.FindingsThe authors find that firms with shareholder activism provide more complex accounting reporting. Further, both types of activism (including Concern & Dispute and Control & Discussion) are positively associated with ARC. The authors also find that the association between shareholder activism and ARC is more pronounced when the firms have a higher level of litigation risk and a higher proportion of institutional ownership. Collectively, the findings suggest that firms with shareholder activism may be under more pressure to disclose more accounting items, leading to more complex accounting reporting.Originality/valueThe study may be informative to regulators considering the costs and benefits of shareholder activism in financial reporting.
Green entrepreneurship in Saudi Arabia: shaping the landscape of the greener economy
Authors: Abdelwahed Nadia A. Abdelmegeed , Al Doghan Mohammed A. , Saraih Ummi Naiemah , Soomro Bahadur Ali 
Published: October 2023

PurposeEntrepreneurship is one of the pillars of economic development and helps to green the economy. This study investigates the factors that affect green entrepreneurship (GE) in small- and medium-sized enterprises (SMEs) in Saudi Arabia.Design/methodology/approachUsing quantitative modes, this study's findings are based on 334 valid cases from Saudi Arabian SME employees.FindingsUsing path analysis, this study’s findings reveal that green entrepreneurial skills (GES), green opportunities (GOs), entrepreneurial motivation (EM), green incentives (GIs), availability of capital (AoC) and green entrepreneurial self-efficacy (GESE) have positive and significant effects on GE. Moreover, GESE mediates GE's relationships with GES, GOs, EM, GIs and AoC.Practical implicationsThis study's findings support the development of green business practices in terms of green business planning, training and skills. The research provides opportunities for green businesses. Policymakers and SME authorities would benefit from this study's findings in producing green goods and services.Originality/valueThis study empirically confirms the role of various factors such as GESE and GE among Saudi Arabian SMEs.
Impact of social media influencers' credibility
on millennial consumers' green purchasing behavior: a concept paper on
personal and social identities
Authors: Rodrigo Arani , Mendis Trevor 
Published: October 2023

Purpose
The purpose of this paper is to provide the theoretical insights with regard
to the green purchasing intention–behavior gap and the role played by
social media influences in abating this gap. This paper takes into
consideration a wider aspect with regard to the antecedents of behavioral
intention through personal and social identities in place of the antecedents
presented in the theory of planned behavior and social-identity theory.
Furthermore, as the theories lack an explanation of how to reduce the
intention–behavior gap, this paper also argues the source credibility
model (SCM) in explaining the impact that social media influences can have
on the behavioral gap.

Design/methodology/approach
Hypothetical deductive method is proposed for this concept paper under the
positivism research paradigm.

Findings
Not applicable as this is a concept paper. However, the paper discusses the
theoretical and managerial implications.

Research
limitations/implications
This is a concept paper. Yes this paper discusses the theoretical,
managerial, and social/ecological implications.

Practical implications
This paper highlights the relevance of consumers' personal and social
identities when consumers make purchasing decisions regarding green
products. How managers can make marketing strategies, based on credibility
model, involving social media influences as product endorsers and
ambassadors, as well as the policy makers to design products, earmark
consumer behavior and to conduct marketing campaigns in time to come.

Social implications
As to how policies can be designed and adopted for bio-based economies where
sustainability and circularity are given priority and to increase the
attention of businesses moving toward sustainable practices.

Originality/value
Original thought developed based on research, theoretical and market
gaps.
Variation in local population size predicts social network structure in wild songbirds
Authors: Beck Kristina B. , Farine Damien R. , Firth Josh A. , Sheldon Ben C. 
Published: October 2023

Abstract

The structure of animal societies is a key determinant of many ecological and evolutionary processes. Yet, we know relatively little about the factors and mechanisms that underpin detailed social structure.

Among other factors, social structure can be influenced by habitat configuration. By shaping animal movement decisions, heterogeneity in habitat features, such as vegetation and the availability of resources, can influence the spatiotemporal distribution of individuals and subsequently key socioecological properties such as the local population size and density. Differences in local population size and density can impact opportunities for social associations and may thus drive substantial variation in local social structure.

Here, we investigated spatiotemporal variation in population size at 65 distinct locations in a small songbird, the great tit (Parus major) and its effect on social network structure. We first explored the within‐location consistency of population size from weekly samples and whether the observed variation in local population size was predicted by the underlying habitat configuration. Next, we created social networks from the birds' foraging associations at each location for each week and examined if local population size affected social structure.

We show that population size is highly repeatable within locations across weeks and years and that some of the observed variation in local population size was predicted by the underlying habitat, with locations closer to the forest edge having on average larger population sizes. Furthermore, we show that local population size affected social structure inferred by four global network metrics. Using simple simulations, we then reveal that much of the observed social structure is shaped by social processes. Across different population sizes, the birds' social structure was largely explained by their preference to forage in flocks. In addition, over and above effects of social foraging, social preferences between birds (i.e. social relationships) shaped certain network features such as the extent of realized social connections.

Our findings thus suggest that individual social decisions substantially contribute to shaping certain social network features over and above effects of population size alone.
Transmisogyny in later (1588–1623) hagiography on Mother Juana de la Cruz (1481–1534)
Authors: Becker Claire 
Published: October 2023

AbstractIn this article, I contextualise and explain the restriction of what I term ‘trans potential’ in later hagiography on the Spanish visionary abbess Mother Juana de la Cruz (1481–1534). The first written work on Juana included a functionally transmasculine narrative (Juana passed briefly as a man) and a functionally transfeminine narrative (God miraculously feminised the previously male Juana prior to her birth, leaving her with an Adam's apple). In later works, the transmasculine narrative appears significantly modified, and the transfeminine narrative does not appear at all. These textual reformulations, I conclude, are most legible within the framework of transmisogyny.
Monocyte‐to‐lymphocyte ratio and gastrointestinal disorder‐related hospitalization in patients on maintenance hemodialysis
Authors: Tian Maolu , Yuan Jing , He Pinghong , Yu Fangfang , Long Changzhu , Zha Yan 
Published: October 2023

AbstractIntroductionTo explore the association between monocyte‐to‐lymphocyte ratio (MLR) and the risk of hospitalization due to gastrointestinal (GI) disorder in hemodialysis (HD) patients.MethodsIn this multicenter, observational cohort study, 1626 patients were enrolled in 2019 and followed up to 2 years. Cox regression models were performed to estimate the association of MLR with GI disorder‐related hospitalization risk. Receiver‐operating characteristic (ROC) analyses were conducted to evaluate the cutoff value of MLR in identifying GI disorder‐related hospitalization.ResultsDuring a median follow‐up of 24 months, GI disorder‐related hospitalization occurred in 107 patients. Higher MLR was independently associated with greater risks of GI disorder‐related hospitalization. Furthermore, a cut‐off value of 0.42 differentiated patients with GI disorder‐related hospitalization from those without GI involvement.ConclusionMLR was associated with the occurrence of GI disorder‐related hospitalization in HD patients. The blood MLR could be monitored as a useful marker to predict GI disorder‐related hospitalization.
Senolytics prevent caveolar CaV3.2‐RyR axis malfunction in old vascular smooth muscle
Authors: Lin Jie , Guo Weiming , Luo Qingtian , Zhang Qingping , Wan Teng , Jiang Changyu , Ye Yuanchun , Lin Haihuan , Fan Gang 
Published: October 2023

AbstractAging is a major risk factor for cardiovascular diseases. Our previous studies demonstrate that aging impairs the caveolar T‐type CaV3.2‐RyR axis for extracellular Ca2+ influx to trigger Ca2+ sparks in vascular smooth muscle cells (VSMCs). We hypothesize that the administration of senolytics, which can selectively clear senescent cells, could preserve the caveolar CaV3.2‐RyR axis in aging VSMCs. In this study, 10‐month‐old mice were administered the senolytics cocktail consisting of dasatinib (5 mg/kg) and quercetin (50 mg/kg) or vehicle bi‐weekly for 4 months. Using VSMCs from mouse mesenteric arteries, we found that Ca2+ sparks were diminished after caveolae disruption by methyl‐β‐cyclodextrin (10 mM) in cells from D + Q treated but not vehicle‐treated 14‐month‐old mice. D + Q treatment promoted the expression of CaV3.2 in 14‐month‐old mesenteric arteries. Structural analysis using electron tomography and immunofluorescence staining revealed the remodeling of caveolae and co‐localization of CaV3.2‐Cav‐1 in D + Q treatment aged mesenteric arteries. In keeping with theoretical observations, Cav3.2 channel inhibition by Ni2+ (50 μM) suppressed Ca2+ in VSMCs from the D + Q group, with no effect observed in vehicle‐treated arteries. Our study provides evidence that age‐related caveolar CaV3.2‐RyR axis malfunction can be alleviated by pharmaceutical intervention targeting cellular senescence. Our findings support the potential of senolytics for ameliorating age‐associated cardiovascular disease.
Mixed ownership reform, political connections, and overinvestment
Authors: Wang Jizhou , He Jin’an , Cebula Richard , Foley Maggie , Peng Fangping 
Published: October 2023

AbstractThe study discovers that mixed ownership reform aimed at enhancing the performance and resource allocation efficiency of state‐owned enterprises may have unintended consequences in China. When the nature of state‐owned control remains unchanged, there is a risk of increased overinvestment due to misaligned interests between state‐owned equity representatives and companies. This incentive can be mitigated by introducing nonstate shareholders with political connections. The study employs a double machine learning method to analyze data from state‐owned listed companies that introduced nonstate shareholders through stock issuance between 2008 and 2019. The research underscores that modern corporate governance mechanisms are crucial for successful mixed ownership reform.