bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital workflow for mini-implant–assisted rapid palatal expander fabrication–a case report
Authors: Hsu Li-Fang , Moon Won , Chen Shih-Chin , Chang Kelvin Wen-Chung 
Published: November 2023

Abstract
Background
Non-surgical mini-implant assisted rapid palatal expansion, or midfacial skeletal expansion, is a paradigm-shifting concept that in recent years has expanded the envelope of orthopedic movement in the transverse direction for adult patients. Although adding mini-screws to a rapid palatal expander is not complicated, accurate and successful expansion strongly depends on the device’s position and its relation to the resisting structures of the maxillofacial complex.

Case presentation
This article presents a digital workflow to locate the optimal position of the Midfacial Skeletal Expander (MSE) device in a CBCT-combined intraoral scan file and describes how to transfer the MSE position intra-orally with properly sized bands during the device fabrication. The complete digital workflow of MSE fabrication and its application for a Class III orthognathic surgical case is presented in detail.

Conclusions
This report describes a completely digital process that can accurately position the MSE device according to the orientation and morphology of maxillary basal bone, which is crucial in adult cases demand maxillary expansion.
Metal nanoparticles as a potential technique for the diagnosis and treatment of gastrointestinal cancer: a comprehensive review
Authors: Roshani Mohammad , Rezaian-Isfahni Arya , Lotfalizadeh Mohammad Hassan , Khassafi Negar , Abadi Mohammad Hassan Jafari Najaf , Nejati Majid 
Published: November 2023

AbstractGastrointestinal (GI) cancer is a major health problem worldwide, and current diagnostic and therapeutic approaches are often inadequate. Various metallic nanoparticles (MNPs) have been widely studied for several biomedical applications, including cancer. They may potentially overcome the challenges associated with conventional chemotherapy and significantly impact the overall survival of GI cancer patients. Functionalized MNPs with targeted ligands provide more efficient localization of tumor energy deposition, better solubility and stability, and specific targeting properties. In addition to enhanced therapeutic efficacy, MNPs are also a diagnostic tool for molecular imaging of malignant lesions, enabling non-invasive imaging or detection of tumor-specific or tumor-associated antigens. MNP-based therapeutic systems enable simultaneous stability and solubility of encapsulated drugs and regulate the delivery of therapeutic agents directly to tumor cells, which improves therapeutic efficacy and minimizes drug toxicity and leakage into normal cells. However, metal nanoparticles have been shown to have a cytotoxic effect on cells in vitro. This can be a concern when using metal nanoparticles for cancer treatment, as they may also kill healthy cells in addition to cancer cells. In this review, we provide an overview of the current state of the field, including preparation methods of MNPs, clinical applications, and advances in their use in targeted GI cancer therapy, as well as the advantages and limitations of using metal nanoparticles for the diagnosis and treatment of gastrointestinal cancer such as potential toxicity. We also discuss potential future directions and areas for further research, including the development of novel MNP-based approaches and the optimization of existing approaches.
Diagnostic performance of ultrasensitive rapid diagnostic test for the detection of Plasmodium falciparum infections in asymptomatic individuals in Kisangani, Northeast Democratic Republic of Congo
Authors: Lupaka Mbumba , Degefa Teshome , Eba Kasahun , Zeynudin Ahmed , Yewhalaw Delenasaw 
Published: November 2023

Abstract
Background
Ultrasensitive rapid diagnostic test (usRDT) was recently developed to improve the detection of low-density Plasmodium falciparum infections. However, its diagnostic performance has not been evaluated in the Democratic Republic of Congo (DRC). This study aims to determine the performance of the usRDT in malaria diagnosis in asymptomatic individuals under field condition in Kisangani, Northeast of DRC.

Methods
A community-based cross-sectional study was carried out from June to August 2022 on 312 asymptomatic individuals residing in the city of Kisangani. Capillary blood samples were collected by finger prick for microscopic examination of thick and thin blood film, RDTs, and nested polymerase chain reaction (PCR). Alere™ Malaria Ag P.f usRDT and conventional RDT (cRDT/SD Bioline Malaria Ag P.f) kits were used for the detection of Plasmodium histidine rich protein 2 (HRP2) antigen as a proxy for the presence of P. falciparum. The diagnostic performance of the usRDT was compared with cRDT, microscopy and PCR.

Results
The prevalence of asymptomatic P. falciparum malaria was 40.4%, 42.0%, 47.1% and 54.2% by cRDT, microscopy, usRDT and PCR, respectively. By using PCR as a reference, usRDT had sensitivity and specificity of 87.0% (95% CI 81.4–91.7) and 100.0% (95% CI 97.5–100.0), respectively, whereas the cRDT had sensitivity and specificity of 74.6% (95% CI 67.3–80.9) and 100% (95% CI 97.1–100.0), respectively. By using microscopy as a reference, usRDT had sensitivity and specificity of 96.9% (95% CI 92.4–99.2) and 89.0% (95% CI 83.5–93.1), respectively, while the cRDT had sensitivity and specificity of 96.2% (95% CI 92.3–98.7) and 100% (95% CI 97.9–100.0), respectively.

Conclusion
The usRDT showed better diagnostic performance with higher sensitivity than the cRDT which is currently in use as point-of-care test. Further research is necessary to assess the access and cost-effectiveness of the usRDTs to use for malaria surveillance.
Ag nanocomposite hydrogels with immune and regenerative microenvironment regulation promote scarless healing of infected wounds
Authors: Zhang Yihui , Kang Jian , Chen Xuan , Zhang Wenkai , Zhang Xiangqi , Yu Wei , Yuan Wei-En 
Published: November 2023

Abstract
Background
Bacterial infection, complex wound microenvironment and persistent inflammation cause delayed wound healing and scar formation, thereby disrupting the normal function and appearance of skin tissue, which is one of the most problematic clinical issues. Although Ag NPs have a strong antibacterial effect, they tend to oxidize and form aggregates in aqueous solution, which reduces their antibacterial efficacy and increases their toxicity to tissues and organs. Current research on scar treatment is limited and mainly relies on growth factors and drugs to reduce inflammation and scar tissue formation. Therefore, there is a need to develop methods that effectively combine drug delivery, antimicrobial and anti-inflammatory agents to modulate the wound microenvironment, promote wound healing, and prevent skin scarring.

Results
Herein, we developed an innovative Ag nanocomposite hydrogel (Ag NCH) by incorporating Ag nanoparticles (Ag NPs) into a matrix formed by linking catechol-modified hyaluronic acid (HA-CA) with 4-arm PEG-SH. The Ag NPs serve dual functions: they act as reservoirs for releasing Ag/Ag+ at the wound site to combat bacterial infections, and they also function as cross-linkers to ensure the sustained release of basic fibroblast growth factor (bFGF). The potent antibacterial effect of the Ag NPs embedded in the hydrogel against S.aureus was validated through comprehensive in vitro and in vivo analyses. The microstructural analysis of the hydrogels and the in vitro release studies confirmed that the Ag NCH possesses smaller pore sizes and facilitates a slower, more sustained release of bFGF. When applied to acute and infected wound sites, the Ag NCH demonstrated remarkable capabilities in reshaping the immune and regenerative microenvironment. It induced a shift from M1 to M2 macrophage polarization, down-regulated the expression of pro-inflammatory factors such as IL-6 and TNF-α, and up-regulated the expression of anti-inflammatory IL-10. Furthermore, the Ag NCH played a crucial role in regulating collagen deposition and alignment, promoting the formation of mature blood vessels, and significantly enhancing tissue reconstruction and scarless wound healing processes.

Conclusions
We think the designed Ag NCH can provide a promising therapeutic strategy for clinical applications in scarless wound healing and antibacterial therapy.

Graphical Abstract
How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis
Authors: Kułak Karolina , Wojciechowska Natalia , Samelak-Czajka Anna , Jackowiak Paulina , Bagniewska-Zadworna Agnieszka 
Published: November 2023

AbstractThe evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem and phloem, are characterized by high specialization, cell heterogeneity, and diverse cell components. During differentiation and maturation, these tissues undergo an irreversible sequence of events, leading to complete protoplast degradation in xylem or partial degradation in phloem, enabling their undisturbed conductive function. Due to the unique nature of vascular tissue, and the poorly understood processes involved in xylem and phloem development, studying the molecular basis of tissue differentiation is challenging. In this review, we focus on methods crucial for gene expression research in conductive tissues, emphasizing the importance of initial anatomical analysis and appropriate material selection. We trace the expansion of molecular techniques in vascular gene expression studies and discuss the application of single-cell RNA sequencing, a high-throughput technique that has revolutionized transcriptomic analysis. We explore how single-cell RNA sequencing will enhance our knowledge of gene expression in conductive tissues.
Bacteremia due to Serratia rubidaea in intensive care unit: a case series
Authors: Mehdi Asma , Trifi Ahlem , Abbes Salma , Seghir Eya , Tlili Bedis , Masseoud Linda , Noussair Azzouz , Ouhibi Asma , Battikh Hajer , Zribi Meriam , Abdellatif Sami 
Published: November 2023

Abstract
Introduction
Bacteremia caused by Serratia rubidaea is seldom mentioned in comparison with other Enterobacteriaceae species. It primarily affects immunocompromised patients undergoing invasive procedures. Furthermore, the incidence, clinical features, and microbiological profile of this pathogen in the intensive care unit are rarely described.

Case presentation
We present four North African case studies of bacteremia in four young female patients admitted to the intensive care unit for ketoacidosis with a history of diabetes mellitus. All four patients developed catheter-related infections complicated by deep vein thrombosis. The catheter site was femoral in all cases, and the main clinical manifestation was poorly tolerated fever. The pathogen was isolated in multiple peripheral blood cultures (> 4) for each patient, showing a similar profile in all cases: resistance to third-generation cephalosporins and sensitivity to aminoglycosides, piperacillin, fluoroquinolones, and folate-pathway inhibitors. Targeted treatment consisted of a combination of ciprofloxacin 400 mg twice per day and trimethoprim/sulfamethoxazole 400/80 mg thrice per day for all four cases. However, in one case, this regimen was switched to amikacin due to adverse effects. The outcomes were favorable in the majority of cases. The patients described in this study were 21, 66, 22, and 27-year-old North African women.

Conclusion
Most of the reported cases shared common risk factors and clinical aspects. Notably, a case of thrombosis complicating a catheter infection caused by Serratia rubidaea has not been previously reported in the literature. Furthermore, this bloodstream infection typically affects deeply immunocompromised patients. However, our four cases, admitted to the intensive care unit for ketoacidosis, only had a history of diabetes mellitus.
HIF-1α promotes kidney organoid vascularization and applications in disease modeling
Authors: Peng Kexin , Xie Wanqin , Wang Tingting , Li Yamei , de Dieu Habimana Jean , Amissah Obed Boadi , Huang Jufang , Chen Yong , Ni Bin , Li Zhiyuan 
Published: November 2023

Abstract
Background
Kidney organoids derived from human pluripotent stem cells (HiPSCs) hold huge applications for drug screening, disease modeling, and cell transplanting therapy. However, these applications are limited since kidney organoid cannot maintain complete morphology and function like human kidney. Kidney organoids are not well differentiated since the core of the organoid lacked oxygen, nutrition, and vasculature, which creates essential niches. Hypoxia-inducible factor-1 α (HIF-1α) serves as a critical regulator in vascularization and cell survival under hypoxia environment. Less is known about the role of HIF-1α in kidney organoids in this regard. This study tried to investigate the effect of HIF-1α in kidney organoid vascularization and related disease modeling.

Methods
For the vascularization study, kidney organoids were generated from human induced pluripotent stem cells. We overexpressed HIF-1α via plasmid transfection or treated DMOG (Dimethyloxallyl Glycine, an agent for HIF-1α stabilization and accumulation) in kidney progenitor cells to detect the endothelium. For the disease modeling study, we treated kidney organoid with cisplatin under hypoxia environment, with additional HIF-1α transfection.

Result
HIF-1α overexpression elicited kidney organoid vascularization. The endothelial cells and angiotool analysis parameters were increased in HIF-1α plasmid-transfected and DMOG-treated organoids. These angiogenesis processes were partially blocked by VEGFR inhibitors, semaxanib or axitinib. Cisplatin-induced kidney injury (Cleaved caspase 3) was protected by HIF-1α through the upregulation of CD31 and SOD2.

Conclusion
We demonstrated that HIF-1α elicited the process of kidney organoid vascularization and protected against cisplatin-induced kidney organoid injury in hypoxia environment.
Artificial pastoral systems: a review of agent-based modelling studies of pastoral systems
Authors: Moritz Mark , Cross Benjamin , Hunter Chelsea E. 
Published: November 2023

AbstractAgent-based modelling allows researchers to build artificial pastoral systems that are spatially explicit and allow for the examination of complex interactions between households, herds, and rangelands over long time periods. However, agent-based modelling also necessarily reduces the complexity of the pastoral systems. The question that we examine in this paper is how researchers model pastoral systems and what artificial pastoral systems they create. To answer that question, we systematically reviewed 35 agent-based modelling studies of pastoral systems. We examined how the studies describe the focal pastoral system, how the focal system is represented in a conceptual model, implemented in computer code, and how it emerges as an artificial pastoral system from the simulations. Our review indicates that most models are built by interdisciplinary teams, integrated into empirical studies of pastoral systems, and use a specific pastoral system as its focal system. The research problems explored in the models range from resource management, wealth dynamics, herd demography, sustainability, adaptation, mobility, and conflict. The artificial pastoral systems that emerge from these agent-based modelling studies mostly confirm current theoretical understandings that are based on empirical studies of pastoral systems. There are a few emergent patterns that have not been validated extensively in empirical studies. We conclude with a discussion of the theoretical, methodological, and practical implications of using agent-based models to create artificial pastoral systems.
Microbiological trends and mortality risk factors of central line-associated bloodstream infections in an academic medical center 2015–2020
Authors: Alwazzeh Marwan Jabr , Alnimr Amani , Al Nassri Samia A. , Alwarthan Sara M. , Alhajri Mashael , AlShehail Bashayer M. , Almubarak Mahdi , Alghamdi Nada S. , Wali Haytham A. 
Published: November 2023

Abstract
Background
Despite tremendous efforts to prevent central line-associated bloodstream infections, they still remain life-threatening complications among hospitalized patients with significant morbidity and mortality worldwide. The emerging antibiotic-resistant bacteria and other risk factors, including patient comorbidities, complicate patient management.

Methods
A single-center retrospective observational study was conducted at King Fahad Hospital of the University, Eastern Province, Saudi Arabia. Hospitalized patients with confirmed central line-associated bloodstream infections between January 2015 and December 2020 were included. The primary objectives were to investigate the trends in antibiotic susceptibility patterns of the causative agents, coexisting comorbid conditions, and other risk factors associated with mortality.

Results
A total of 214 patients with confirmed central line-associated bloodstream infections were included (CLABSI). The overall 30-day mortality rate was 33.6%. The infection rates per 1000 central line days for medical, surgical, and pediatric intensive care units were 4.97, 2.99, and 4.56 per 1000 CL days, respectively. The overall microbiological trends showed a predominance of Gram-negative agents, a steady increase of fungal CLABSI up to 24.0% in 2020, and a high prevalence of multidrug resistance up to 47% of bacterial CLABSI. In addition, the study indicates a significant negative surviving correlation with diabetes mellitus, cardiovascular disease, lung disease, chronic kidney disease, and the presence of ≥ 3 comorbidities (P < 0.05).

Conclusion
The microbiological trends of the study population demonstrated a steady increase of CLABSI caused by Candida spp. with a predominance of Gram-negative pathogens. Stratifying the patients according to relevant mortality risk factors, including patient comorbidities, will help reduce CLABSI rates and improve patient outcomes.
Inpatient hospital course and self-reported symptomatology in underweight adults with ARFID compared to age- and sex-matched controls with anorexia nervosa
Authors: Vanzhula Irina A. , Wang Erin , Martinelli Mary K. , Schreyer Colleen , Guarda Angela S. 
Published: November 2023

Abstract
Objective
Avoidant restrictive food intake disorder (ARFID) has similar prevalence to anorexia nervosa (AN) in adults, but research in this population is lacking. Although inpatient or residential treatment involving nutritional rehabilitation is increasingly recommended for malnourished individuals with ARFID, best practices remain poorly defined. Existing studies on self-reported symptomatology and treatment course and outcome are primarily in child and adolescent cohorts and demonstrate inconsistent findings. This study aimed to compare hospital course and self-reported symptomatology of underweight adult inpatients with ARFID and sex- and age-matched patients with AN.

Method
Underweight adult patients with ARFID or AN admitted to a specialized, hospital-based behavioral treatment program completed measures of body dissatisfaction, drive for thinness, bulimic symptoms, anxiety, depression, and personality traits. Demographic and treatment course data were abstracted from electronic medical records. Patients with ARFID (n = 69) were matched to those with AN (n = 69) based on sex and age.

Results
Adults with ARFID were closer to target weight at admission, but gained weight at a slower rate, were discharged at lower BMI, and were less likely to reach target weight by discharge than adults with AN. Patients with ARFID reported less weight and shape-related eating disorder, state anxiety, and depression symptoms and lower neuroticism.

Discussion
Adults with ARFID progress through treatment more slowly and achieve less favorable weight outcomes by hospital discharge than patients with AN, but long-term outcomes are unclear. Describing clinical presentations and course of illness of adult ARFID may help inform treatment protocols.
COVID-19, multiple emergencies, and moral entanglements: extraordinary and transcendental moral worlds as a new analytical framework
Authors: Ali Inayat 
Published: November 2023

AbstractThe COVID-19 pandemic resulted in a multitude of complex emergencies, extending beyond the realm of health and healthcare. The situation presented a significant challenge to human security. The content analysis and literature review provide evidence of varied choices and decision-making processes at individual, local, national, and global levels in response to the COVID-19 pandemic. These decisions were influenced by various factors such as time constraints, limited resources, unfamiliar situations, unpredictable outcomes, and the uncertainty surrounding the end of the pandemic. The necessity to make exceptional moral decisions posed a challenge to existing conceptual and analytical frameworks, including Arthur Kleinman’s concept of “local moral worlds.” An examination of emergency medicine, particularly in the context of a critical global event, revealed the presence of numerous ethical decisions that, I suggest, may better be termed extraordinary local moral worlds and transcendental moral worlds. Following the development of an effective vaccine and the official declaration of the end of the pandemic, a thorough assessment of these impacts has commenced at various levels. We have observed a growing sense of regret regarding numerous decisions made amidst this exceptional circumstance. Not only can individuals regret over their choices, but governments and stakeholders, including the World Health Organization (WHO), may also find themselves regretting decisions that were initially seen as pragmatic in containing the virus and flattening the curve. Individuals can also experience regret over their choices. To illustrate the exceptional impacts of the pandemic on “ordinary people”, I specifically draw upon the case of thalassemia patients from Pakistan as these patients required blood to survive, thus, they serve as a significant example of making certain choices. Lastly, I contend that it is crucial to reexamine these choices in order to gain valuable insights that can inform more moral and practical choices in the future.
An improved constraint-inference approach for causality exploration of power system transient stability
Authors: Zhou Yibo , An Jun , Mu Gang , Shi Yan 
Published: November 2023

AbstractTransient stability is the key aspect of power system dynamic security assessment, and data-driven methods are becoming alternative measures of assessment. The current data-driven methods only construct correlations between variables while neglecting causal relationships. Therefore, they face problems such as poor robustness, which restrict their practical application. This paper introduces an improved constraint-inference approach for causality exploration of power system transient stability. Firstly, a causal structure discovery method of power system transient stability is proposed based on a PC-IGCI algorithm, which addresses the shortage caused by Markov equivalence and massive variables. Then, a relative average causal effect index is proposed to reveal the relationship between relative intervention strength and causal effects. The results of a case study verify that the proposed method can identify the causal structure between the transient stability variables entirely based on data. In addition, the causal effect sorting between “cause” and “outcome” of transient stability variables is revealed. This paper provides a new approach for data mining to uncover the causal mechanisms between variables in power systems and expand the capabilities of data-driven methods in power system application.