bluuebunny/crossref_metadata_2025_split huggingface dataset:
NIPBL-mediated RAD21 facilitates tumorigenicity by the PI3K pathway in non-small-cell lung cancer
Authors: Xu Xiaoling , Wang Ding , Xu Weizhen , Li Huihui , Chen Ning , Li Na , Yao Qifeng , Chen Wei , Zhong Jianxiang , Mao Weimin 
Published: February 2024

AbstractIt is urgent to identify novel early diagnostic markers and therapeutic targets for non-small-cell lung cancer (NSCLC), which accounts for 85% of lung cancer cases and has a 5-year survival rate of 4–17%. Here, chromatin immunoprecipitation (ChIP) was used to identify DNA‒protein interactions, RNA methylation was determined by methylated RNA immunoprecipitation (MeRIP), RNA stability was tested by an RNA decay assay. We showed that RAD21, a member of the cohesin complex, is upregulated in NSCLC tissues and cell lines and found to be an independent prognostic factor for overall survival (OS) of NSCLC patients. Mechanistically, the cohesin loading factor Nipped-B-Like Protein (NIPBL) promoted RAD21 gene transcription by enhancing histone H3 lysine 27 (H3K27) demethylation via recruiting lysine demethylase 6B (KDM6B) to the RAD21 gene promoter. RAD21 enhanced phosphatidylinositol 3-kinase (PI3K) gene transcription, and NIPBL reversed the effect of enhancer of zeste 2; catalytic subunit of polycomb repressive complex 2 (EZH2) on RAD21-mediated PI3K gene transcription by disrupting the association between EZH2 and RAD21. Moreover, NIPBL level was increased by stabilization of its transcripts through mRNA methylation. These findings highlight the oncogenic role of RAD21 in NSCLC and suggest its use as a potential diagnostic marker and therapeutic target for NSCLC.
Downregulation of praja2 restrains endocytosis and boosts tyrosine kinase receptors in kidney cancer
Authors: Rinaldi Laura , Chiuso Francesco , Senatore Emanuela , Borzacchiello Domenica , Lignitto Luca , Iannucci Rosa , Donne Rossella Delle , Fuggi Mariano , Reale Carla , Russo Filomena , Russo Nicola Antonino , Giurato Giorgio , Rizzo Francesca , Sellitto Assunta , Santangelo Michele , De Biase Davide , Paciello Orlando , D’Ambrosio Chiara , Amente Stefano , Garbi Corrado , Dalla Emiliano , Scaloni Andrea , Weisz Alessandro , Ambrosino Concetta , Insabato Luigi , Feliciello Antonio 
Published: February 2024

AbstractClear cell renal cell carcinoma (ccRCC) is the most common kidney cancer in the adult population. Late diagnosis, resistance to therapeutics and recurrence of metastatic lesions account for the highest mortality rate among kidney cancer patients. Identifying novel biomarkers for early cancer detection and elucidating the mechanisms underlying ccRCC will provide clues to treat this aggressive malignant tumor. Here, we report that the ubiquitin ligase praja2 forms a complex with-and ubiquitylates the AP2 adapter complex, contributing to receptor endocytosis and clearance. In human RCC tissues and cells, downregulation of praja2 by oncogenic miRNAs (oncomiRs) and the proteasome markedly impairs endocytosis and clearance of the epidermal growth factor receptor (EGFR), and amplifies downstream mitogenic and proliferative signaling. Restoring praja2 levels in RCC cells downregulates EGFR, rewires cancer cell metabolism and ultimately inhibits tumor cell growth and metastasis. Accordingly, genetic ablation of praja2 in mice upregulates RTKs (i.e. EGFR and VEGFR) and induces epithelial and vascular alterations in the kidney tissue.In summary, our findings identify a regulatory loop between oncomiRs and the ubiquitin proteasome system that finely controls RTKs endocytosis and clearance, positively impacting mitogenic signaling and kidney cancer growth.
In-situ gelation of fibrin gel encapsulating platelet-rich plasma-derived exosomes promotes rotator cuff healing
Authors: Li Molin , Shi Lin , Chen Xianghui , Yi Dan , Ding Yufei , Chen Jian , Xing Guanghui , Chen Siming , Wang Li , Zhang Yongyi , Zhu Yaqiong , Wang Yuexiang 
Published: February 2024

AbstractAlthough platelet-rich plasma-derived exosomes (PRP-Exos) hold significant repair potential, their efficacy in treating rotator cuff tear (RCT) remains unknown. In light of the potential for clinical translation of fibrin gel and PRP-Exos, we evaluated their combined impact on RCT healing and explored suitable gel implantation techniques. In vitro experiments demonstrated that PRP-Exos effectively enhanced key phenotypes changes in tendon stem/progenitor cells. Multi-modality imaging, including conventional ultrasound, shear wave elastography ultrasound, and micro-computed tomography, and histopathological assessments were performed to collectively evaluate the regenerative effects on RCT. The regenerated tendons exhibited a well-ordered structure, while bone and cartilage regeneration were significantly improved. PRP-Exos participated in the healing process of RCT. In-situ gelation of fibrin gel-encapsulated PRP-Exos at the bone-tendon interface during surgery proved to be a feasible gel implantation method that benefits the healing outcome. Comprehensive multi-modality postoperative evaluations were necessary, providing a reliable foundation for post-injury repair.
Linking ATP and allosteric sites to achieve superadditive binding with bivalent EGFR kinase inhibitors
Authors: Wittlinger Florian , Ogboo Blessing C. , Shevchenko Ekaterina , Damghani Tahereh , Pham Calvin D. , Schaeffner Ilse K. , Oligny Brandon T. , Chitnis Surbhi P. , Beyett Tyler S. , Rasch Alexander , Buckley Brian , Urul Daniel A. , Shaurova Tatiana , May Earl W. , Schaefer Erik M. , Eck Michael J. , Hershberger Pamela A. , Poso Antti , Laufer Stefan A. , Heppner David E. 
Published: February 2024

AbstractBivalent molecules consisting of groups connected through bridging linkers often exhibit strong target binding and unique biological effects. However, developing bivalent inhibitors with the desired activity is challenging due to the dual motif architecture of these molecules and the variability that can be introduced through differing linker structures and geometries. We report a set of alternatively linked bivalent EGFR inhibitors that simultaneously occupy the ATP substrate and allosteric pockets. Crystal structures show that initial and redesigned linkers bridging a trisubstituted imidazole ATP-site inhibitor and dibenzodiazepinone allosteric-site inhibitor proved successful in spanning these sites. The re-engineered linker yielded a compound that exhibited significantly higher potency (~60 pM) against the drug-resistant EGFR L858R/T790M and L858R/T790M/C797S, which was superadditive as compared with the parent molecules. The enhanced potency is attributed to factors stemming from the linker connection to the allosteric-site group and informs strategies to engineer linkers in bivalent agent design.
Catalytic thiolation-depolymerization-like decomposition of oxyphenylene-type super engineering plastics via selective carbon–oxygen main chain cleavages
Authors: Minami Yasunori , Imamura Sae , Matsuyama Nao , Nakajima Yumiko , Yoshida Masaru 
Published: February 2024

AbstractAs the effective use of carbon resources has become a pressing societal issue, the importance of chemical recycling of plastics has increased. The catalytic chemical decomposition for plastics is a promising approach for creating valuable products under efficient and mild conditions. Although several commodity and engineering plastics have been applied, the decompositions of stable resins composed of strong main chains such as polyamides, thermoset resins, and super engineering plastics are underdeveloped. Especially, super engineering plastics that have high heat resistance, chemical resistance, and low solubility are nearly unexplored. In addition, many super engineering plastics are composed of robust aromatic ethers, which are difficult to cleave. Herein, we report the catalytic depolymerization-like chemical decomposition of oxyphenylene-based super engineering plastics such as polyetheretherketone and polysulfone using thiols via selective carbon–oxygen main chain cleavage to form electron-deficient arenes with sulfur functional groups and bisphenols. The catalyst combination of a bulky phosphazene base P4-tBu with inorganic bases such as tripotassium phosphate enabled smooth decomposition. This method could be utilized with carbon- or glass fiber-enforced polyetheretherketone materials and a consumer resin. The sulfur functional groups in one product could be transformed to amino and sulfonium groups and fluorine by using suitable catalysts.
Machine learning modelling for predicting the utilization of invasive and non‐invasive ventilation throughout the ICU duration
Authors: Schwager Emma , Nabian Mohsen , Liu Xinggang , Feng Ting , French Robin , Amelung Pam , Atallah Louis , Badawi Omar 
Published: February 2024

AbstractThe goal of this work is to develop a Machine Learning model to predict the need for both invasive and non‐invasive mechanical ventilation in intensive care unit (ICU) patients. Using the Philips eICU Research Institute (ERI) database, 2.6 million ICU patient data from 2010 to 2019 were analyzed. This data was randomly split into training (63%), validation (27%), and test (10%) sets. Additionally, an external test set from a single hospital from the ERI database was employed to assess the model's generalizability. Model performance was determined by comparing the model probability predictions with the actual incidence of ventilation use, either invasive or non‐invasive. The model demonstrated a prediction performance with an AUC of 0.921 for overall ventilation, 0.937 for invasive, and 0.827 for non‐invasive. Factors such as high Glasgow Coma Scores, younger age, lower BMI, and lower PaCO2 were highlighted as indicators of a lower likelihood for the need for ventilation. The model can serve as a retrospective benchmarking tool for hospitals to assess ICU performance concerning mechanical ventilation necessity. It also enables analysis of ventilation strategy trends and risk‐adjusted comparisons, with potential for future testing as a clinical decision tool for optimizing ICU ventilation management.
Comparative analysis of the hybrid gazelle‐Nelder–Mead algorithm for parameter extraction and optimization of solar photovoltaic systems
Authors: Ekinci Serdar , Izci Davut , Hussien Abdelazim G. 
Published: February 2024

AbstractThe pressing need for sustainable energy solutions has driven significant research in optimizing solar photovoltaic (PV) systems which is crucial for maximizing energy conversion efficiency. Here, a novel hybrid gazelle‐Nelder–Mead (GOANM) algorithm is proposed and evaluated. The GOANM algorithm synergistically integrates the gazelle optimization algorithm (GOA) with the Nelder–Mead (NM) algorithm, offering an efficient and powerful approach for parameter extraction in solar PV models. This investigation involves a thorough assessment of the algorithm's performance across diverse benchmark functions, including unimodal, multimodal, fixed‐dimensional multimodal, and CEC2020 benchmark functions. Notably, the GOANM consistently outperforms other optimization approaches, demonstrating enhanced convergence speed, accuracy, and reliability. Furthermore, the application of the GOANM is extended to the parameter extraction of the single diode and double diode models of RTC France solar cell and PV model of Photowatt‐PWP201 PV module. The experimental results consistently demonstrate that the GOANM outperforms other optimization approaches in terms of accurate parameter estimation, low root mean square values, fast convergence, and alignment with experimental data. These results emphasize its role in achieving superior performance and efficiency in renewable energy systems.
A data‐driven identification method for impedance stability analysis of inverter‐based resources
Authors: Wang Hongyi , Sun Pingyang , Sahebkar Farkhani Jalal , Chen Zhe 
Published: February 2024

AbstractObtaining inverter controller information may be a premise for seeking its dynamic behaviour. But accurate knowledge of such information would be unrealistic for real functioning inverter‐interfaced generators (IIGs), which hinders the stability analysis of the IIG. A new data‐driven impedance identification method is proposed for stability analysis, which involves an improved sparse identification algorithm as an ancillary function within the system identification framework. It contains mainly two design stages. First, the transform basis matrix (TBM) is devised systematically as a prior knowledge library to contain the possibly existing control structures. In the second stage, a sparse identification algorithm is reformulated in order to extract the relevant structures in TBM while obtaining controller parameters. The authors demonstrate that the sparse vector between the TBM and output signal is closely related to the controller structure. The effectiveness of the proposed method is verified on grid‐connected inverters based on droop control and virtual synchronous machine control.
Rheological characteristics and behaviour prediction of lubricating grease for RV reducer across a wide temperature range
Authors: Jiang Benchi , Zhou Yansheng , Tu Zhijian , Pan Jiabao 
Published: February 2024

AbstractGrease in the normal operation of the rotate vector (RV) reducer has a role that cannot be ignored, for the variable working conditions of the RV reducer, the performance of the lubricant changes directly affect its reliable operation. Therefore, the study of the rheological properties of the grease has become the focus of the study of RV reducer performance. Here, SK‐1A grease is taken as the research object, and its rheological characteristics under wide temperature range working conditions (−20–40°C) are investigated through rheological experiments to analyze the potential influence of the performance of RV reducer. However, the ordinary way of research is too complicated to better research the rheological properties of grease for a variety of working conditions. The Elman neural network (ENN) model was used to predict the rheological properties, and the results were compared with those of back propagation (BP) and radial basis function (RBF) neural networks. The results demonstrate that the ENN model demonstrates high prediction accuracy for grease rheological property prediction by comparing three types of predictions. This method can provide a theoretical reference for the accurate prediction of the rheological properties of lubricating grease affected by complex multifactors.
Accretion of primordial H–He atmospheres in mini-Neptunes: The importance of envelope enrichment
Authors: Mol Lous M. , Mordasini C. , Helled R. 
Published: February 2024

Context. Out of the more than 5000 detected exoplanets, a considerable number belong to a category called “mini-Neptunes”. Interior models of these planets suggest that they have primordial H–He-dominated atmospheres. As this type of planet is not found in the Solar System, understanding their formation is a key challenge in planet formation theory. Unfortunately, quantifying how much H–He planets have, based on their observed mass and radius, is impossible due to the degeneracy of interior models.
Aims. Another approach to estimating the range of possible primordial envelope masses is to use formation theory. As different assumptions in planet formation can heavily influence the nebular gas accretion rate of small planets, it is unclear how large the envelope of a protoplanet should be. We explore the effects that different assumptions regarding planet formation have on the nebular gas accretion rate, particularly by exploring the way in which solid material interacts with the envelope. This allows us to estimate the range of possible post-formation primordial envelopes. Thereby, we demonstrate the impact of envelope enrichment on the initial primordial envelope, which can be used in evolution models.
Methods. We applied formation models that include different solid accretion rate prescriptions. Our assumption is that mini-Neptunes form beyond the ice line and migrate inward after formation; thus, we formed planets in situ at 3 and 5 au. We considered that the envelope can be enriched by the accreted solids in the form of water. We studied how different assumptions and parameters influence the ratio between the planet’s total mass and the fraction of primordial gas.
Results. The primordial envelope fractions for low- and intermediate-mass planets (total mass below 15 M⊕) can range from 0.1% to 50%. Envelope enrichment can lead to higher primordial mass fractions. We find that the solid accretion rate timescale has the largest influence on the primordial envelope size.
Conclusions. Rates of primordial gas accretion onto small planets can span many orders of magnitude. Planet formation models need to use a self-consistent gas accretion prescription.
Designing an experimental method for assessing biocompatibility of circuit coatings using biomarkers for platelet activation during cardiopulmonary bypass
Authors: Sancheti Meghal , Rentschler Mitchell , Bolch Charlotte , Li Weidang , Necco Katelyn , Rath Thomas , Esfandiarei Mitra , Darban Nathaniel 
Published: February 2024

Introduction: Cardiopulmonary bypass is an essential component of cardiothoracic surgeries. However, significant complications such as systemic inflammatory response syndrome (SIRS) resulting from cardiopulmonary bypass (CPB) are a common occurrence due to contact between circulating blood and foreign surfaces that leads to platelet activation. It is suggested that different available CPB circuit coatings can potentially reduce platelet activation. However, there have been no published evidence-based reports confirming these claims. In addition, there is no well-established protocol for studying platelet activation biomarkers during CPBin vitroin a laboratory setting.Methods: CPB was simulated in the laboratory using bovine blood in two different types of coated CPB circuits:Trillium®Biosurfaceby Medtronic, andXcoatingTMSurface by Terumo. Fresh bovine blood samples were collected and circulated through the CPB circuit following the standard protocol used in the operation rooms. Blood samples were then collected at 5 min, 30 min, and 55 min during the circulation. Blood plasmas were separated and subjected to enzyme-linked immunosorbent assay to measure most established platelet activation markers P-selectin, Platelet Factor 4 (PF4), Glycoprotein IIb/IIIa (GPIIb/IIIa), and β-thromboglobulin (β-TG) at different time points.Results: The biomarker values at 30 min and 55 min were compared to the base values at 5 min for each type of CPB circuit. The results of the means from all measured biomarkers showed data measurements that indicated no significant variability within each coating. All collected data points fell within ±2 SD of the means, which was considered acceptable variations across technical replicates. Conclusion: In this study, we were able to establish anin vitroprotocol in the laboratory setting that is precise and reliable with minimum intra-variability. This established protocol will allow for future studies in which different coated CPB circuits can be compared for their effectiveness in blocking platelet activation during the CPB.
Infusionswärmer zur Hämotherapie in der
Notfallmedizin: Ist die schnelle Gabe gewärmter Blutprodukte
prähospital möglich?
Authors: Rudolph Marcus , Knapp Jürgen , Sitzmann Nicole , Braun Jörg , Reifferscheid Florian 
Published: February 2024

ZusammenfassungIm hämorrhagischen Schock erfolgt die zielgerichtete Therapie durch die
schnelle Transfusion von Blutprodukten. Diese sollten erwärmt appliziert
werden, um eine Hypothermie mit Koagulopathie zu verhindern. Hierzu,
müssen geeignete Maßnahmen ergriffen werden.Die folgende Arbeit untersucht die Eignung handelsüblicher mobiler
Blutwärmer für die präklinische Transfusion.
Blutwärmer mit einem Wärmemodul reduzieren die Durchflussrate
und sind somit nur eingeschränkt für eine Notfalltransfusion
geeignet. Die Auswahl eines geeigneten Wärmegeräts spielt somit
eine wichtige Rolle für eine schnelle Applikation im Notfall.
Promotional language in radiology publications: increasing use of “excellent”, “favorable”, “promising”, “robust”, and “unique”
Authors: Kwee Thomas Christian , Kwee Robert Michael 
Published: February 2024

Purpose To investigate if radiology researchers are increasingly promoting their scientific findings by more frequently using positive words in their publications.
Materials and Methods This study included all articles that were published in 14 general radiology journals between 2003 and 2022. The title and abstract of each article were assessed for the presence of positive, negative, neutral, and random words, according to predefined sets of words for each category. Usage of positive, negative, neutral, and random words was calculated for each year and corrected for the total number of articles in each year. Temporal trends between 2002 and 2023 and the relationship between positive word usage and journal impact factor (IF) were assessed.
Results Positive word usage (Mann-Kendall tau of 0.895, P< 0.001) and neutral word usage (Mann-Kendall tau of 0.463, P = 0.005) showed significant upward temporal trends. Negative word usage and random word usage did not show any significant temporal trends. Five positive words showed significantly increased usage over time and were present in more than 1 % of titles/abstracts in at least one year: “excellent” (Mann-Kendall tau of 0.800, P< 0.001), “favorable” (Mann-Kendall tau of 0.547, P< 0.001), “promising” (Mann-Kendall tau of 0.607, P< 0.001), “robust” (Mann-Kendall tau of 0.737, P< 0.001), and “unique” (Mann-Kendall tau of 0.747, P< 0.001). There was no significant association between positive word usage and journal IF.
Conclusion Radiology researchers appear to increasingly promote their scientific findings by more frequently using positive words in their publications over the past two decades.
Key Points
Citation Format
Chronische Schmerzen im Alter – psychologische Aspekte und
ihre therapeutischen Konsequenzen*
Authors: Mattenklodt Peter , Ingenhorst Anne , Flatau Brigitta , Grießinger Norbert 
Published: February 2024

ZusammenfassungChronische Schmerzen im Alter gehen mit gravierenden gesundheitlichen
Auswirkungen einher. Internationale Leitlinien fordern daher, dass die
Schmerztherapie Älterer bevorzugt als interdisziplinäre
multimodale Therapie auf der Basis eines bio-psycho-sozialen Schmerzmodells
erfolgen sollte. Für das interdisziplinäre Schmerzassessment
liegen spezifische Testverfahren und Interviewleitfäden vor. Die Evidenz
für die Wirksamkeit einer interdisziplinären multimodalen
Schmerztherapie bei Älteren ist noch begrenzt. Kontrollierte klinische
Studien konnten zeigen, dass diese Patienten von einer solchen Therapie
profitieren – insbesondere, wenn die Therapie an ihre spezifischen
Bedürfnisse angepasst wird. Im psychologischen Training sind
insbesondere Schmerzakzeptanz, Balancierung von Ruhe und Aktivität,
soziale Integration und der Umgang mit dem Älterwerden wichtige Themen.
Auch Entspannungs- und Achtsamkeitsverfahren können die Schmerzen und
die Funktion günstig beeinflussen, sind bei Patienten beliebt und werden
oft in das alltägliche Schmerzmanagement übernommen.
Schmerzedukation als begleitende Maßnahme gilt als sinnvoll und kann
auch bei Älteren zunehmend durch digitale Medien unterstützt
werden. Zur Reduzierung von Angst-Vermeidungs-Überzeugungen empfiehlt
sich ein konfrontationstherapeutisches Vorgehen nach den Prinzipien einer
Angsttherapie („AMIKA“). Da unklar ist, wie lange die erreichten
therapeutischen Effekte anhalten, kommt der Nachsorge bei der Therapie
älterer Patienten besondere Bedeutung zu.