bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating changes in firefighter urinary metabolomes after structural fires: an untargeted, high resolution approach
Authors: Furlong Melissa A. , Liu Tuo , Snider Justin M. , Tfaily Malak M. , Itson Christian , Beitel Shawn , Parsawar Krishna , Keck Kristen , Galligan James , Walker Douglas I. , Gulotta John J. , Burgess Jefferey L. 
Published: November 2023

AbstractFirefighters have elevated rates of urinary tract cancers and other adverse health outcomes, which may be attributable to environmental occupational exposures. Untargeted metabolomics was applied to characterize this suite of environmental exposures and biological changes in response to occupational firefighting. 200 urine samples from 100 firefighters collected at baseline and two to four hours post-fire were analyzed using untargeted liquid-chromatography and high-resolution mass spectrometry. Changes in metabolite abundance after a fire were estimated with fixed effects linear regression, with false discovery rate (FDR) adjustment. Partial least squares discriminant analysis (PLS-DA) was also used, and variable important projection (VIP) scores were extracted. Systemic changes were evaluated using pathway enrichment for highly discriminating metabolites. Metabolome-wide-association-study (MWAS) identified 268 metabolites associated with firefighting activity at FDR q < 0.05. Of these, 20 were annotated with high confidence, including the amino acids taurine, proline, and betaine; the indoles kynurenic acid and indole-3-acetic acid; the known uremic toxins trimethylamine n-oxide and hippuric acid; and the hormone 7a-hydroxytestosterone. Partial least squares discriminant analysis (PLS-DA) additionally implicated choline, cortisol, and other hormones. Significant pathways included metabolism of urea cycle/amino group, alanine and aspartate, aspartate and asparagine, vitamin b3 (nicotinate and nicotinamide), and arginine and proline. Firefighters show a broad metabolic response to fires, including altered excretion of indole compounds and uremic toxins. Implicated pathways and features, particularly uremic toxins, may be important regulators of firefighter’s increased risk for urinary tract cancers.
Exploring ceRNA networks for key biomarkers in breast cancer subtypes and immune regulation
Authors: Shariatmadar Taleghani Alireza , Zohrab Beigi Yasaman , Zare-Mirakabad Fatemeh , Masoudi-Nejad Ali 
Published: November 2023

AbstractBreast cancer is a major global health concern, and recent researches have highlighted the critical roles of non-coding RNAs in both cancer and the immune system. The competing endogenous RNA hypothesis suggests that various types of RNA, including coding and non-coding RNAs, compete for microRNA targets, acting as molecular sponges. This study introduces the Pre_CLM_BCS pipeline to investigate the potential of long non-coding RNAs and circular RNAs as biomarkers in breast cancer subtypes. The pipeline identifies specific modules within each subtype that contain at least one long non-coding RNA or circular RNA exhibiting significantly distinct expression patterns when compared to other subtypes. The results reveal potential biomarker genes for each subtype, such as circ_001845, circ_001124, circ_003925, circ_000736, and circ_003996 for the basal-like subtype, circ_00306 and circ_00128 for the luminal B subtype, circ_000709 and NPHS1 for the normal-like subtype, CAMKV and circ_001855 for the luminal A subtype, and circ_00128 and circ_00173 for the HER2+ subtype. Additionally, certain long non-coding RNAs and circular RNAs, including RGS5-AS1, C6orf223, HHLA3-AS1, circ_000349, circ_003996, circ_003925, circ_002665, circ_001855, and DLEU1, are identified as potential regulators of T cell mechanisms, underscoring their importance in understanding breast cancer progression in various subtypes. This pipeline provides valuable insights into cancer and immune-related processes in breast cancer subtypes.
A comparative cradle-to-gate life cycle assessment of three cotton stalk waste sustainable applications
Authors: Ibrahim Rana Adel , Inan Hatice , Fahim Irene S. 
Published: November 2023

AbstractThis paper presents a novel approach to utilizing agricultural waste. It compares three different applications for cotton stalks: fabrication of wood composites, bioethanol production, and biogas cradle-to-gate Life cycle assessment production processes. Cotton cultivation generates a lot of debris, mostly cotton stalks, which are incinerated or landfilled, Sustainable resource management is critical for maintaining the ecosystem, and economic stability, and promoting social fairness since it ensures the long-term availability of resources while minimizing environmental damage. The investigation uses the Ecological Footprint, Impact 2002 +, Global Warming Damage Potential, Greenhouse Gas Protocol, Recipe Midpoint, Ecosystem Damage Potential, and CML IA Baseline—open LCA-enabled environmental sustainability assessments. The analysis showed that bioethanol has a lower carbon footprint and climate change impact than both wood composite and biogas production processes, as a result, this could cause a preference for bioethanol production as an environmentally friendly strategy for cotton stalks utilization. While human toxicity was higher in the biogas production process, it emits less fossil CO2 than biogenic CO2. The total climate change of wood composite, bioethanol, and biogas production processes was 0.01761, 0.011300, and 0.01083 points, respectively. This research helps accomplish wider ecological and economic aims by giving insights into sustainable waste management practices.
Ecological correlates and predictors of Lassa fever incidence in Ondo State, Nigeria 2017–2021: an emerging urban trend
Authors: Cadmus Simeon , Taiwo Olalekan John , Akinseye Victor , Cadmus Eniola , Famokun Gboyega , Fagbemi Stephen , Ansumana Rashid , Omoluabi Anddy , Ayinmode Adekunle , Oluwayelu Daniel , Odemuyiwa Solomon , Tomori Oyewale 
Published: November 2023

AbstractLassa fever (LF) is prevalent in many West African countries, including Nigeria. Efforts to combat LF have primarily focused on rural areas where interactions between rodents and humans are common. However, recent studies indicate a shift in its occurrence from rural to urban areas. We analysed secondary data of reported LF outbreaks from 2017 to 2021 in Ondo State, Nigeria to identify the distribution pattern, ecological variations, and other determinants of disease spread from the ward level using nearest neighbour statistics and regression analysis. Data utilised include LF incidence, ecological variables involving population, nighttime light intensity, vegetation, temperature, market presence, road length, and building area coverage. ArcGIS Pro 3.0 software was employed for spatial analysis. Results revealed spatio-temporal clustering of LF incidents between 2017 and 2021, with an increasing trend followed by a decline in 2021. All wards in Owo Local Government Area were identified as LF hotspots. The ecological variables exhibited significant correlations with the number of LF cases in the wards, except for maximum temperature. Notably, these variables varied significantly between wards with confirmed LF and those without. Therefore, it is important to prioritise strategies for mitigating LF outbreaks in urban areas of Nigeria and other LF-endemic countries.
Long noncoding RNA NONHSAT160169.1 promotes resistance via hsa-let-7c-3p/SOX2 axis in gastric cancer
Authors: Zhao Xuan , Xu Zijian , Meng Bi , Ren Tong , Wang Xu , Hou Rui , Li Sijin , Ma Wen , Liu Dan , Zheng Junnian , Shi Ming 
Published: November 2023

AbstractIn clinical trials involving patients with HER2 (ERBB2 receptor tyrosine kinase 2) positive gastric cancer, the efficacy of the HER2-targeted drug lapatinib has proven to be disappointingly poor. Under the persistent pressure exerted by targeted drug therapy, a subset of tumor cells exhibit acquired drug resistance through the activation of novel survival signaling cascades, alongside the proliferation of tumor cells that previously harbored mutations conferring resistance to the drug. This study was undertaken with the aim of elucidating in comprehensive detail the intricate mechanisms behind adaptive resistance and identifying novel therapeutic targets that hold promise in the development of effective lapatinib-based therapies for the specific subset of patients afflicted with gastric cancer. We have successfully established a gastric cancer cell line with acquired lapatinib resistance, designated as HGC-27-LR cells. Utilizing comprehensive coding and noncoding transcriptome sequencing analysis, we have identified key factors that regulate lapatinib resistance in HGC-27 cells. We have compellingly validated that among all the lncRNAs identified in HGC-27-LR cells, a novel lncRNA (long noncoding RNA) named NONHSAT160169.1 was found to be most notably upregulated following exposure to lapatinib treatment. The upregulation of NONHSAT160169.1 significantly augmented the migratory, invasive, and stemness capabilities of HGC-27-LR cells. Furthermore, we have delved into the mechanism by which NONHSAT160169.1 regulates lapatinib resistance. The findings have revealed that NONHSAT160169.1, which is induced by the p-STAT3 (signal transducer and activator of transcription 3) nuclear transport pathway, functions as a decoy that competitively interacts with hsa-let-7c-3p and thereby abrogates the inhibitory effect of hsa-let-7c-3p on SOX2 (SRY-box transcription factor 2) expression. Hence, our study has unveiled the NONHSAT160169.1/hsa-let-7c-3p/SOX2 signaling pathway as a novel and pivotal axis for comprehending and surmounting lapatinib resistance in the treatment of HER2-positive gastric cancer.
A human bispecific antibody neutralizes botulinum neurotoxin serotype A
Authors: Lu Jiansheng , Jiang Yujia , Guo Jiazheng , Chen Lei , Liu Fujia , Li Zhiying , Liu Xuyang , Du Peng , Yu Yunzhou , Wang Rong , Yang Zhixin 
Published: November 2023

AbstractBotulinum neurotoxin (BoNT) shows high lethality and toxicity, marking it as an important biological threat. The only effective post-exposure therapy is botulinum antitoxin; however, such products have great potential for improvement. To prevent or treat BoNT, monoclonal antibodies (mAbs) are promising agents. Herein, we aimed to construct a bispecific antibody (termed LUZ-A1-A3) based on the anti-BoNT/A human monoclonal antibodies (HMAb) A1 and A3. LUZ-A1-A3 binds to the Hc and L-HN domains of BoNT/A, displaying potent neutralization activity against BoNT/A (124 × higher than that of HMAb A1 or HMAb A3 alone and 15 × higher than that of the A1 + A3 combination). LUZ-A1-A3 provided effective protection against BoNT/A in an in vivo mouse model. Mice were protected from infection with 500 × LD50 of BoNT/A by LUZ-A1-A3 from up to 7 days before intraperitoneal administration of BoNT/A. We also demonstrated the effective therapeutic capacity of LUZ-A1-A3 against BoNT/A in a mouse model. LUZ-A1-A3 (5 μg/mouse) neutralized 20 × LD50 of BoNT/A at 3 h after intraperitoneal BoNT/A administration and complete neutralized 20 × LD50 of BoNT/A at 0.5 h after intraperitoneal BoNT/A administration. Thus, LUZ-A1-A3 is a promising agent for the pre-exposure prophylaxis and post-exposure treatment of BoNT/A.
Sea level anomalies affect the ocean circulation at abyssal depths
Authors: Frey D. I. , Morozov E. G. , Smirnova D. A. 
Published: November 2023

AbstractAbyssal channels are the key points controlling bottom circulation of the World Ocean. They provide meridional transport of the coldest Antarctic Bottom Water between deep-water basins influencing the meridional overturning circulation and the climate on a global scale. Here we show that the synoptic variability of deep-water flows including blocking abyssal currents between deep ocean basins is related to sea level anomalies observed over the channels. Our results demonstrate that processes at the ocean surface have a more significant connection with the bottom circulation than it was considered earlier. This study opens a discussion of the importance of mesoscale eddies and air-sea interactions on water exchange between abyssal basins, meridional heat transport in the ocean, and possible responses of the ocean to the observed sea level rise in a changing climate.
Label-free quantitative proteomics analysis for type 2 diabetes mellitus early diagnostic marker discovery using data-independent acquisition mass spectrometry (DIA-MS)
Authors: Nimer Refat M. , Alfaqih Mahmoud A. , Shehabat Eman R. , Mujammami Muhammad , Abdel Rahman Anas M. 
Published: November 2023

AbstractType-2 diabetes mellitus (T2DM) therapy requires early diagnosis and complication avoidance. Unfortunately, current diagnostic markers do not meet these needs. Data-independent acquisition mass spectrometry (DIA-MS) offers a solution for clinical diagnosis, providing reliable and precise sample quantification. This study utilized DIA-MS to investigate proteomic differential expression in the serum of recently diagnosed T2DM patients. The study conducted a comparative protein expression analysis between healthy and recently diagnosed T2DM groups (discovery cohort). A candidate protein was then validated using enzyme-linked immune assay (ELISA) on serum samples collected from T2DM patients (n = 87) and healthy control (n = 60) (validation cohort). A total of 1074 proteins were identified, and 90 were significantly dysregulated between the two groups, including 32 newly associated with T2DM. Among these proteins, the expression of S100 calcium-binding protein A6 (S100A6) was validated by ELISA. It showed a significant increase in T2DM samples compared to the control group. It was evaluated as a biomarker using the receiver operating characteristic (ROC) curve, consistent with the DIA-MS results. Novel proteins are reported to be involved in the development and progression of T2DM. Further studies are required to investigate the differential expression of candidate marker proteins in a larger population of T2DM patients.
Fault-tolerant attitude control of the satellite in the presence of simultaneous actuator and sensor faults
Authors: Fazlyab Ali Reza , Fani Saberi Farhad , Kabganian Mansour 
Published: November 2023

AbstractIn this paper, a robust attitude control algorithm is developed based on backstepping sliding mode control for a satellite using reaction wheels and thrusters that can perform its mission despite faulty actuators. In this method, the actuator dynamics have been considered to design the controller and the asymptotic stability of the proposed algorithm has been proven based on the Lyapunov theory. The designed controller can converge the attitude of the system into the desired path in the presence of faulty actuators. Then a fault-tolerant attitude estimation system is designed based on federated unscented Kalman filters that can be effectively employed to detect and isolate sensor faults. Finally, the performance of the designed attitude estimation and controller is investigated by simulation in the presence of both actuator and sensor faults.
Citronella essential oil-based nanoemulsion as a post-emergence natural herbicide
Authors: Somala Naphat , Laosinwattana Chamroon , Chotsaeng Nawasit , Teerarak Montinee 
Published: November 2023

AbstractA natural herbicide nanoemulsion was fabricated from citronella (Cymbopogon nardus L.) essential oil (CEO) and a nonionic surfactant Tween 60 mixed with Span 60 at hydrophilic-lipophilic balance 14 using a microfluidization method. The main constituents of CEO were citronellol (35.244%), geraniol (21.906%), and citronellal (13.632%). CEO nanoemulsion droplet size and polydispersity index (PI) were evaluated by dynamic light scattering (DLS). The smallest droplet size (33.2 nm, PI 0.135) was obtained from a microfluidizer at 20,000 psi, 7 cycles. Nanoemulsion droplet in transmission electron microscopy correlated with DLS confirmed CEO to successfully produce nanoemulsion. The herbicidal activity of the nanoemulsion as a foliar spray was evaluated against Echinochloa cruss-galli and Amaranthus tricolor as representative narrow- and broadleaf weed plants, both of which presented visual toxicity symptoms. The modes of action of the nanoemulsion were then determined in terms of membrane integrity (relative electrolyte leakage; REL), malondialdehyde (MDA), and photosynthetic pigment contents. The results showed increase in REL and MDA which indicated the destruction of the treated plants; additionally, chlorophylls and carotenoid contents were decreased. Consequently, CEO nanoemulsion may have the possibility to act as a natural herbicide resource, and natural herbicides from citronella nanoemulsions could be good alternatives for use in sustainable agriculture.
Bioaccumulation and biomagnification of heavy metals in marine micro-predators
Authors: Danovaro Roberto , Cocozza di Montanara Adele , Corinaldesi Cinzia , Dell’Anno Antonio , Illuminati Silvia , Willis Trevor J. , Gambi Cristina 
Published: November 2023

AbstractNematodes represent >3/5 of the abundance of the world’s metazoans and usually account for nearly 90% of the total benthic fauna, playing a key ecological role in the benthic ecosystem functioning on a global scale. These small metazoans include a relevant number of microscopic predators and, in turn, are the most abundant preys of macro-megafauna and fish juveniles thus playing a key role in marine food webs. Here, using two independent approaches, we test the bioaccumulation in marine nematodes of several heavy metals present in contaminated sediments. We report here that nematodes, despite their short life cycle and small size, bioaccumulate significantly heavy metals. Bioaccumulation increases from deposit feeders and microalgal grazers to predators of microbes and other tiny metazoans. These results suggest that nematodes also contribute to their biomagnification along the food webs and can contribute to increase the transfer of contaminants from the sediments to larger organisms.
Digital twin‐based online resilience scheduling for microgrids: An approach combining imitative learning and deep reinforcement learning
Authors: Sun Haonan , Liu Nian , Tan Lu , Du Peng , Zhang Bocheng 
Published: November 2023

AbstractStrong uncertainty of renewables puts high demands on the fast response of flexibility resources and resilience‐oriented optimal scheduling for microgrids (MGs). Digital twins (DT) technology based on data‐driven methods is a potential solution to this problem. A DT‐based online resilience scheduling framework for MGs is designed in this study. Based on the proposed framework, a hybrid sequential‐parallel combination method of imitation learning (IL) and deep reinforcement learning (DRL) is proposed to develop the optimal scheduling strategy for MGs. First, a mixed integer second‐order cone programming (MISOCP) model is adopted to behave as an expert to generate decision demonstrations corresponding to operation scenarios of MGs. Then, IL and deep deterministic policy gradient (DDPG) are combined by (1) sequential pretrain and finetune and (2) parallel experience storing and sampling two steps to learn the optimal policy for MGs. Expert demonstrations from the MISOCP model are utilized to pretrain a deep neural network model by IL and initialize the policy network of DDPG to avoid it learning from scratch. Moreover, an expert replay buffer is introduced specifically to avoid forgetting the well‐behaved expert experience and to further accelerate the training process. A 6‐bus MG test system case study demonstrates the effectiveness and scalability of the proposed approach.
Device-assisted enteroscopy in the surveillance of intestinal hamartomas in Peutz-Jeghers syndrome
Authors: Mohamed Elfeky Omar Wahid , Panjwani Suraj , Cave David , Wild Daniel , Raines Daniel 
Published: November 2023

Abstract
Background and study aims Peutz-Jeghers syndrome (PJS) is an
autosomal-dominant genetic disorder characterized by the formation of hamartomatous polyps in
the gastrointestinal tract. These polyps result in significant morbidity due to adverse events
(AEs) including intestinal obstruction, bleeding, and malignancy. The aim of this study was to
describe the role of device-assisted enteroscopy (DAE) in monitoring and prophylactic
polypectomy within the small bowel.
Patients and methods Electronic medical records were surveyed to
identify all DAE procedures performed in patients with PJS at three US referral centers
between January 1, 2007 and January 1, 2020. Individual charts were reviewed to collect and
analyze specific data points. Primary end points included AEs associated with DAE-related
polypectomy and the rate of laparotomy in PJS patients prior to, and following, index DAE.
Secondary data points included patient characteristics, procedural details, and
size/location/distribution of small bowel hamartomas.
Results Twenty-three patients met our inclusion criteria. Of
these, 18 (75%) had previously undergone small bowel surgery prior to index DAE. Between 2007
and 2020, 46 DAEs were performed in these patients with an average of one exam every 2.5
years. A total of 131 polypectomies were performed with an AE rate of 1.5%. None of our cohort
required emergent surgery related to AEs of small bowel hamartomas over 336 years of
aggregated follow-up.
Conclusions Endoscopic management of small bowel polyps in
patients with PJS using DAE is an effective strategy for prophylactic removal of hamartomas.
DAE surveillance and endoscopic polypectomy is safe and may decrease the need for repeated
laparotomy in patients with PJS.
Regioselective Double C–H Functionalization of Arenes via Aryl Thianthrenium Salt Analogues
Authors: Hamad Aboubakr , Mrozowicz Michał , Ritter Tobias , Xie Yuanhao 
Published: November 2023

AbstractDirected C–H functionalization reactions are powerful tools for the rapid and selective syntheses of complex molecules. However, many existing C–H functionalization reactions require the presence of a preinstalled directing group that has to be either a part of the molecule or introduced onto an existing functional group. Here, we report analogues of thianthrene that can also function as directing groups for intermediate directed C–H functionalization and thereby permit regioselective 2,4- and 3,4-aromatic C–H difunctionalization of simple arenes in yields of 22–33% over three steps.