bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary glycine supplementation improves the growth performance of 110- to 240-g (phase II) hybrid striped bass (Morone saxatilis ♀× Morone chrysops ♂) fed soybean meal-based diets
Authors: He Wenliang , Li Xinyu , Wu Guoyao 
Published: December 2023

Abstract
We recently reported that supplementing glycine to soybean meal (SBM)-based diets is necessary for optimum growth of 5- to 40-g (phase I) hybrid striped bass (HSB). The present study tested the hypothesis that supplementing glycine to SBM-based diets may enhance the growth of 110- to 240-g (phase II) HSB. HSB (the initial body weight of approximately 110 g) were fed an SBM (58%)-based diet supplemented with 0%, 1%, or 2% of glycine, with l-alanine serving as the isonitrogenous control. There were four tanks per dietary group, with four fish per tank. The fish were fed their respective diets to apparent satiation twice daily. The feed intake and body weight of fish were recorded daily and every 2 wk, respectively. At the end of the 56-d feeding trial, plasma and tissue samples were collected to determine amino acid concentrations and histological alterations, and tissues were used to measure the oxidation of l-glutamate, l-glutamine, l-aspartate, and glycine. Results showed that dietary supplementation with 1% and 2% glycine dose-dependently increased (P &lt; 0.05) the concentration of glycine in the plasma of HSB by 48% and 99%, respectively. Compared with the 0%-glycine group, dietary supplementation with 1% glycine did not affect (P &gt; 0.05) the feed intake of HSB but increased (P &lt; 0.05) their final body weight, weight gain, and gain:feed ratio during the whole period by 13%, 29%, and 21%, respectively. Compared with the 1% glycine group, dietary supplementation with 2% glycine increased (P &lt; 0.05) the feed intake, final body weight, and weight gain of HSB by 13%, 7%, and 14%, respectively. Compared with the 0%-glycine group, fish fed with the 1%-glycine and 2%-glycine diets had a greater (P &lt; 0.05) villus height in the proximal intestine, when compared with the 0%-glycine group. Collectively, these results indicated that SBM-based diets did not provide sufficient glycine for phase II HSB (110 to 240 g) and that dietary glycine supplementation is essential for their optimum growth and intestinal structure.
The twolined spittlebug (Hemiptera: Cercopidae) invades Hawaiʻi: establishment, biology, and management of a destructive forage grass pest
Authors: Wilson Shannon , Thorne Mark S , Wright Mark G , Peck Daniel C , Mack Jennifer , Fukumoto Glen K , Curtiss RT 
Published: December 2023

Abstract
The twolined spittlebug, Prosapia bicincta (Say) (Hemiptera: Cercopidae), is a major economic pest of turfgrass, forage grass, and sugarcane. Native to the southeast United States, it impacts rangelands and the landscape trade by damaging pastures, golf courses, urban landscapes, and lawns. This insect pest was reported and confirmed in Hawaiʻi for the first time in 2016, the first species in the family Cercopidae to invade Hawaiʻi. On Hawaiʻi Island, P. bicincta is severely devastating Hawaiʻi’s beef cattle industry. Since its initial detection, P. bicincta has rapidly expanded its range across an estimated 72,183 ha area on Hawaiʻi Island, and it continues to disperse to new locations. In areas of high infestation, this pest has caused 100% dieback of key pasture grasses for livestock forage, including kikuyu (Pennisetum clandestinum Hochst. Ex Chiov) and pangola (Digitaria eriantha Steud) grasses, leading to the establishment of low-quality forage grasses and weeds. Additionally, this pest may affect the Hawaiʻi landscape trade and impact golf courses, urban landscapes, and homeowners. The taxonomy, detection history, range, biology, ecology, economic damage, and management strategies for P. bicincta are discussed.
“After completing the 2011 population census, I found myself in court for the first time”
Authors: Radojevic Gordana 
Published: December 2023

Abstract
Gordana Radojevic was dismissed from her position as director of the Statistical Office of Montenegro in 2021 and suffered almost 2 years of intense governmental scrutiny and “political disgrace”. The court annulled the decision on her dismissal in August 2023. Having now cleared her name, she shares her story
Falling parachutes: Are politicians becoming more “local”?
Authors: Gandy Rob , Foster Scott 
Published: December 2023

Abstract
As UK political parties gear up for a likely general election in 2024, they are busy selecting the most suitable candidates for each seat. Psephologists say voters prefer their parliamentarians to be “local”, not “parachuted” in – but what does the data teach us? Rob Gandy and Scott Foster catch the Speaker's eye
Chronic kidney disease and risk of kidney or urothelial malignancy: systematic review and meta-analysis
Authors: Brooks Emily R , Siriruchatanon Mutita , Prabhu Vinay , Charytan David M , Huang William C , Chen Yu , Kang Stella K 
Published: December 2023

ABSTRACT

Background
Chronic kidney disease (CKD) is highly prevalent, affecting approximately 11% of US adults. Multiple studies have evaluated a potential association between CKD and urinary tract malignancies. Summary estimates of urinary tract malignancy risk in CKD patients with and without common co-existing conditions may guide clinical practice recommendations.

Methods
Four electronic databases were searched for original cohort studies evaluating the association between CKD and urinary tract cancers (kidney cancer and urothelial carcinoma) through 25 May 2023, in persons with at least moderate CKD and no dialysis or kidney transplantation. Quality assessment was performed for studies meeting inclusion criteria using the Newcastle-Ottawa Scale. Meta-analysis with a random-effects model was performed for unadjusted incidence rate ratios (IRR) as well as adjusted hazard ratios (aHR) for confounding conditions (diabetes, hypertension and/or tobacco use), shown to have association with kidney cancer and urothelial carcinoma. Sub-analysis was conducted for estimates associated with CKD stages separately.

Results
Six cohort studies with 8 617 563 persons were included. Overall, the methodological quality of the studies was good. CKD was associated with both higher unadjusted incidence and adjusted hazard of kidney cancer (IRR 3.36, 95% confidence interval (CI) 2.32–4.88; aHR 2.04, 95% CI 1.77–2.36) and urothelial cancer (IRR 3.96, 95% CI 2.44–6.40; aHR 1.35, 95% CI 1.22–1.50) compared with persons without CKD. Examining incident urinary tract cancers by CKD severity, risks were elevated in stage 3 CKD (kidney aHR 1.89, 95% CI 1.56–2.30; urothelial carcinoma aHR 1.35, 95% CI 1.20–1.52) as well as in stages 4/5 CKD (kidney cancer aHR 2.30, 95% CI 2.00–2.66; urothelial carcinoma aHR 1.24, 95% CI 1.04–1.49).

Conclusions
Even moderate CKD is associated with elevated risk of kidney cancer and urothelial carcinoma. Providers should consider these elevated risks when managing individuals with CKD, particularly when considering evaluation for the presence and etiology of hematuria.
Arabidopsis Calmodulin-like Proteins CML13 and CML14 Interact with Calmodulin-Binding Transcriptional Activators and Function in Salinity Stress Response
Authors: Hau Bryan , Symonds Kyle , Teresinski Howard , Janssen Abby , Duff Liam , Smith Milena , Benidickson Kirsten , Plaxton William , Snedden Wayne A 
Published: December 2023

Abstract
Eukaryotic cells use calcium ions (Ca2+) as second messengers, particularly in response to abiotic and biotic stresses. These signals are detected by Ca2+ sensor proteins, such as calmodulin (CaM), which regulate the downstream target proteins. Plants also possess many CaM-like proteins (CMLs), most of which remain unstudied. We recently demonstrated that Arabidopsis CML13 and CML14 interact with proteins containing isoleucine/glutamine (IQ) domains, including CaM-binding transcriptional activators (CAMTAs). Here, we show that CaM, CML13 and CML14 bind all six members of the Arabidopsis CAMTA family. Using a combination of in planta and in vitro protein-interaction assays, we tested 11 members of the CaM/CML family and demonstrated that only CaM, CML13 and CML14 bind to CAMTA IQ domains. CaM, CML13 and CML14 showed Ca2+-independent binding to the IQ region of CAMTA6 and CAMTA3, and CAMTA6 in vitro exhibited some specificity toward individual IQ domains within CAMTA6 in split-luciferase in planta assays. We show that cml13 mutants exhibited enhanced salinity tolerance during germination compared to wild-type plants, a phenotype similar to camta6 mutants. In contrast, plants overexpressing CML13-GFP or CML14-GFP in the wild-type background showed increased NaCl sensitivity. Under mannitol stress, cml13 mutants were more susceptible than camta6 mutants or wild-type plants. The phenotype of cml13 mutants could be rescued with the wild-type CML13 gene. Several salinity-marker genes under CAMTA6 control were similarly misregulated in both camta6 and cml13 mutants, further supporting a role for CML13 in CAMTA6 function. Collectively, our data suggest that CML13 and CML14 participate in abiotic stress signaling as CAMTA effectors.
Shifting Attitudes towards Domestic Violence: The Impact of Primary Education on Women’s Marital Outcomes in Benin
Authors: Deschênes Sarah , Hotte Rozenn 
Published: December 2023

Abstract
The paper examines the effect of a primary education program in Benin on women’s marital outcomes. The study leverages a sharp increase in the construction of schools in the 1990s to assess the causal impact of an increase in primary-school supply on primary-school attendance, employment, marital outcomes, and experience and tolerance of intimate partner violence (IPV). Using quasi-experimental geographical and historical variations in the number of schools built, the results indicate that in rural areas the school building program increased the probability of attending primary school and increased the age at marriage and at first child. It decreased the probability that women find domestic violence justified and that they experience emotional IPV. The effects are driven by women’s own increase in education rather than their husbands’.
A Near Real-Time Risk Analytics Algorithm Predicts Elevated Lactate Levels in Pediatric Cardiac Critical Care Patients
Authors: Asfari Ahmed , Wolovits Joshua , Gazit Avihu Z. , Abbas Qalab , Macfadyen Andrew J. , Cooper David S. , Futterman Craig , Penk Jamie S. , Kelly Robert B. , Salvin Joshua W. , Borasino Santiago , Zaccagni Hayden J 
Published: December 2023

BACKGROUND:
Postoperative pediatric congenital heart patients are predisposed to develop low-cardiac output syndrome. Serum lactate (lactic acid [LA]) is a well-defined marker of inadequate systemic oxygen delivery.

OBJECTIVES:
We hypothesized that a near real-time risk index calculated by a noninvasive predictive analytics algorithm predicts elevated LA in pediatric patients admitted to a cardiac ICU (CICU).

DERIVATION COHORT:
Ten tertiary CICUs in the United States and Pakistan.

VALIDATION COHORT:
Retrospective observational study performed to validate a hyperlactatemia (HLA) index using T3 platform data (Etiometry, Boston, MA) from pediatric patients less than or equal to 12 years of age admitted to CICU (n = 3,496) from January 1, 2018, to December 31, 2020. Patients lacking required data for module or LA measurements were excluded.

PREDICTION MODEL:
Physiologic algorithm used to calculate an HLA index that incorporates physiologic data from patients in a CICU. The algorithm uses Bayes’ theorem to interpret newly acquired data in a near real-time manner given its own previous assessment of the physiologic state of the patient.

RESULTS:
A total of 58,168 LA measurements were obtained from 3,496 patients included in a validation dataset. HLA was defined as LA level greater than 4 mmol/L. Using receiver operating characteristic analysis and a complete dataset, the HLA index predicted HLA with high sensitivity and specificity (area under the curve 0.95). As the index value increased, the likelihood of having higher LA increased (p < 0.01). In the validation dataset, the relative risk of having LA greater than 4 mmol/L when the HLA index is less than 1 is 0.07 (95% CI: 0.06-0.08), and the relative risk of having LA less than 4 mmol/L when the HLA index greater than 99 is 0.13 (95% CI, 0.12–0.14).

CONCLUSIONS:
These results validate the capacity of the HLA index. This novel index can provide a noninvasive prediction of elevated LA. The HLA index showed strong positive association with elevated LA levels, potentially providing bedside clinicians with an early, noninvasive warning of impaired cardiac output and oxygen delivery. Prospective studies are required to analyze the effect of this index on clinical decision-making and outcomes in pediatric population.
Pathologic Complete Response After Gastric Artery Chemoembolization Combined With Tislelizumab for Neoadjuvant Therapy of Locally Advanced Gastric Cancer: A Case Report
Authors: Lin Yufu , Chen Yabo , Lin Shenggan , Zheng Jingmei , Zhang Xiuping , Gan Lu 
Published: December 2023

Gastric cancer is the most common type of gastrointestinal cancer in China which about 80% of patients are locally advanced or advanced when diagnosed. Surgery along brings high recurrence rate for locally advanced gastric cancer (LAGC), and neoadjuvant therapies are needed. The use of programmed cell death-1 (PD-1)/programmed death-ligand 1 inhibitor nowadays improved the disease-free survival for LAGC, however, only <35% of patients achieved pathologic complete response (pCR) after neoadjuvant therapy nowadays. Therefore, new regimens are needed to be investigated. Gastric artery chemoembolization is applied to metastasis gastric cancer and researches showed interventional therapy can enhance the antitumor effect of PD-1 inhibitor. Here, for the first time, we combined gastric artery chemoembolization with tislelizumab (a PD-1 inhibitor) for neoadjuvant therapy of a patient with LAGC. The patient achieved pCR after a D2 resection and tumor regression grade score was 1. After surgery, the patient received tislelizumab 200 mg per 3 weeks, and showed no sign of recurrence after 6 months of follow-up. The study indicated the use of tislelizumab and gastric artery chemoembolization for neoadjuvant therapy may bring a better pCR rate and prognosis of LAGC.
Systematic Review of Morbidity and Mortality Meeting Standardization: Does It Lead to Improved Professional Development, System Improvements, Clinician Engagement, and Enhanced Patient Safety Culture?
Authors: Steel Emily J. , Janda Monika , Jamali Shayaun , Winning Michelle , Dai Bryan , Sellwood Kylie 
Published: December 2023

Objectives
This systematic review sought to better understand the effect of standardized Morbidity and Mortality meetings (M&Ms) on learning, system improvement, clinician engagement, and patient safety culture.

Methods
Three electronic databases were searched using a range of text words, synonyms, and subject headings to identify the major concepts of M&M meetings. Articles published between October 2012 (the end date of an earlier review) and February 2021 were assessed against the inclusion criteria, and thematic synthesis was conducted on the included studies.

Results
After abstract and full-text review in Covidence, from 824 studies identified, 16 met the eligibility criteria. Studies were mostly surveys (n = 13) and evaluated effectiveness primarily from the perspectives of M&M chairs and participants, rather than assessment of objective improvement in patient outcomes. The most prevalent themes relating to the standardization of M&M processes were case selection (n = 15) and administration (n = 12). The objectives of quality improvement and education were equally prevalent (12 studies each), but several studies reported that these 2 objectives as conflicting rather than complementary. Clinician engagement, patient safety culture, and organizational governance and leadership were identified as facilitators of effective M&Ms.

Conclusions
There is insufficient evidence to guide best practice in M&Ms, but standardized structures and processes implemented with organizational leadership and administrative support are associated with M&Ms that address objectives related to learning and system improvement. Standardization of the structures and processes of M&Ms is perceived differently depending on participants’ role and discipline, and clinician engagement is critical to support a culture of safety and quality improvement.
Diagnostic Accuracy of Contrast-Enhanced Thoracic Photon-Counting Computed Tomography for Opportunistic Locoregional Staging of Breast Cancer Compared With Digital Mammography
Authors: Neubauer Jakob , Wilpert Caroline , Gebler Oliver , Taran Florin-Andrei , Pichotka Martin , Stein Thomas , Molina-Fuentes Moisés Felipe , Weiss Jakob , Juhasz-Böss Ingolf , Bamberg Fabian , Windfuhr-Blum Marisa , Neubauer Claudia 
Published: December 2023

Objective
Accurate locoregional staging is crucial for effective breast cancer treatment. Photon-counting computed tomography (PC-CT) is an emerging technology with high spatial resolution and the ability to depict uptake of contrast agents in tissues, making it a promising tool for breast cancer imaging. The aim of this study was to establish the feasibility of locoregional staging of breast cancer through contrast-enhanced thoracic PC-CT, assess its diagnostic performance, and compare it with that of digital mammography (DM).

Materials and Methods
Patients with newly diagnosed breast cancer, DM, and indication of thoracic CT staging were prospectively enrolled in this clinical cohort study over a period of 6 months. Participants underwent contrast-enhanced thoracic PC-CT and breast magnetic resonance imaging in prone position. After blinding to patient data, 2 radiologists independently rated PC-CT and DM regarding the following 6 characteristics: (1) diameter of the largest mass lesion, (2) infiltration of cutis/pectoral muscle/thoracic wall, (3) number of mass lesions, (4) presence/absence of adjacent ductal carcinoma in situ (DCIS), (5) tumor conspicuity, and (6) diagnostic confidence. Reference standard was generated from consensus reading of magnetic resonance imaging combined with all histopathological/clinical data by an independent adjudication committee applying TNM eighth edition.

Results
Among 32 enrolled female subjects (mean ± SD age, 59 ± 13.0 years), diagnostic accuracy for T-classification was higher for PC-CT compared with DM (0.94 vs 0.50, P < 0.01). Moreover, the correlation of the number of detected tumor masses with the reference standard was stronger for PC-CT than for DM (0.72 vs 0.50, P < 0.01). We observed that PC-CT significantly (P < 0.04) outperformed DM regarding not only sensitivity (0.83 and 0.25, respectively) but also specificity (0.99 and 0.80, respectively) for adjacent DCIS. The κ values for interreader reliability were higher for PC-CT compared with DM (mean 0.88 vs 0.54, respectively; P = 0.01).

Conclusions
Photon-counting computed tomography outperformed DM in T-classification and provided higher diagnostic accuracy for the detection of adjacent DCIS. Therefore, opportunistic locoregional staging of breast cancer in contrast-enhanced thoracic PC-CT is feasible and could overcome limitations of DM with the potential to improve patient management.
A targeted metabolomic method to detect epigenetically relevant metabolites
Authors: Miro-Blanch J. , Junza A. , Capellades J. , Balvay A. , Maudet C. , Kovatcheva M. , Raineri S. , Rabot S. , Mellor J. , Serrano M. , Yanes O. 
Published: December 2023

AbstractMetabolites play a central role in the chemical crosstalk between metabolism and epigenetic marks. Epigenetically relevant metabolites are substrates, products and cofactors that can act as activators or inhibitors of epigenetic enzymes, which control gene expression by adding or removing chemical marks in the DNA, RNA and histones. Diet composition, and biosynthetic pathways encoded in the gut microbiome and the host genome are the main sources of these metabolites for mammals. Despite the increasing interest in the study of the ‘microbiota-nutrient metabolism-host epigenetic axis’ to understand health and disease, there is a lack of a sensitive and easy analytical method to detect epigenetically relevant metabolites simultaneously. Here, we show an straightforward biphasic extraction where the organic phase is directly analyzed by GC-EI MS to detect short-chain fatty acids and formate without chemical derivatization, and the aqueous phase is analyzed by HILIC coupled to ESI-MS/MS, which together can cover >30 epigenetically relevant metabolites in biological samples such as liver, plasma or feces. In addition, we propose a stable isotope tracing method based on multiple-reaction monitoring (MRM) transitions by LC-QqQ MS to understand how13C-labeled glucose or glutamine are used to build SAM and acetyl-CoA, the main methyl and acetyl group donors in epigenetic modifications, respectively. We anticipate that our methods will complement epigenomic and proteomic analyses adding another layer of molecular information towards mechanistic insights.HighlightsHost and microbiota metabolites link metabolism with epigenetic regulation.Chemical structure diversity in epigenetically relevant metabolites challenges its analysis with a single method.A biphasic extraction with no chemical derivatization is able to recover SCFAs and other epigenetically relevant metabolites.A novel isotope trace experiment approach allows isotopomer resolution using MS2 data.
Categorising potential non-disasters
Authors: Podloski Brady , Kelman Ilan 
Published: December 2023

PurposeThis short paper builds on and critiques work presenting potential non-disasters: disasters that did not seem to happen despite a major hazard. Previous work does not differentiate among different types of potential non-disasters. This short paper uses local information to propose three categories according to reasons for vulnerability being low or absent. These proposed categories are used to critique the construct of “potential non-disasters”.Design/methodology/approachThis short paper uses a subjective approach to examples of potential non-disasters in 2022, focusing on local information that describes what happened. This information is applied and analysed for the three proposed categories using examples from Japan, Nepal, the Philippines and Vietnam. Such comparisons are useful for critiquing “potential non-disasters”, by understanding better local approaches and information available for reporting on situations that could be disasters.FindingsPotential non-disasters remain relevant for exploring mechanisms, tools and actions for educating about vulnerability causes and vulnerability reduction to avert disasters. Limitations are evident by relying on media reports, even local ones with local authors. A suggestion is to implement a grant programme for collecting data immediately after a major hazard without an evident, major disaster. Additionally, an annual report and critique of each year's potential non-disasters, categorised and analysed, would help to evidence the presence and limits of the “potential non-disaster” construct.Originality/valueThis short paper contributes a much deeper theoretical dive into understanding potential non-disasters, both describing them and the drawbacks of the construct. To practitioners, the construct now offers more avenues for actions while illustrating their effectiveness in reducing vulnerabilities. Thus, this paper supports multiple, linked pathways towards more non-disasters.