bluuebunny/crossref_metadata_2025_split huggingface dataset:
La integración de conocimientos teórico-prácticos desde la experiencia de profesionales del Trabajo Social en la formación del estudiantado a través de metodologías activas y participativas
Authors: Medina-Rodríguez María del Valle , Álvarez Bernardo Gloria , Mielgo García Francisco 
Published: January 2024

En los estudios de grado de Trabajo Social, tal y como establece el Libro Blanco del Título de Trabajo Social, se establecen una serie de objetivos competenciales relacionados con los conocimientos teóricos, metodológicos y actitudinales contemplados en el currículum formativo de la disciplina. La incorporación de metodologías activas y participativas a los procesos de enseñanza-aprendizaje favorecen la adquisición de estos en el estudiantado. Igualmente, en los procesos de enseñanza-aprendizaje la utilización de recursos didácticos que conecten los conocimientos teóricos con el ejercicio de la práctica profesional favorece la comprensión de las materias curriculares en el estudiantado. Este estudio responde al objetivo de analizar el impacto que tiene la teoría en la intervención social para trabajadoras y trabajadores sociales en activo para la creación de contenido teórico-práctico para la asignatura de Introducción a los Modelos de Trabajo Social de la Universidad de Granada. La metodología empleada incorpora el trabajo documental y el desarrollo de tres grupos de discusión en el que han participado 18 profesionales del trabajo social en activo. El análisis de los discursos que se generaron en los mismos ha servido como recurso para el desarrollo de una clase invertida con el estudiantado que cursa la asignatura de Introducción a los Modelos de Trabajo Social de la Universidad de Granada. El análisis se ha realizado en torno a tres categorías: el valor de la teoría en el ejercicio profesional; roles, fines y modelos teóricos subyacentes al trabajo social; y el trabajo social del futuro. Los resultados muestran cómo los y las profesionales del trabajo social dotan de gran importancia a la teoría como guía en su práctica profesional, abordando la misma preferentemente desde modelos teóricos sistémicos y humanistas y apostando por la recuperación de enfoques teóricos comunitarios y críticos-transformadores.
Altered O-linked glycosylation in benign and malignant meningiomas
Authors: Talabnin Chutima , Trasaktaweesakul Thanawat , Jaturutthaweechot Pitchanun , Asavaritikrai Pundit , Kongnawakun Dusit , Silsirivanit Atit , Araki Norie , Talabnin Krajang 
Published: January 2024

Background
Changes in protein glycosylation have been reported in various diseases, including cancer; however, the consequences of altered glycosylation in meningiomas remains undefined. We established two benign meningioma cell lines—SUT-MG12 and SUT-MG14, WHO grade I—and demonstrated the glycan and glycosyltransferase profiles of the mucin-type O-linked glycosylation in the primary benign meningioma cells compared with two malignant meningioma cell lines—HKBMM and IOMM-Lee, WHO grade III. Changes in O-linked glycosylation profiles in malignant meningiomas were proposed.

Methods
Primary culture technique, morphological analysis, and immunocytochemistry were used to establish and characterize two benign meningioma cell lines. The glycan profiles of the primary benign and malignant meningiomas cell lines were then analyzed using lectin cytochemistry. The gene expression of O-linked glycosyltransferases, mucins, sialyltransferases, and fucosyltransferases were analyzed in benign and malignant meningioma using the GEO database (GEO series GSE16581) and quantitative-PCR (qPCR).

Results
Lectin cytochemistry revealed that the terminal galactose (Gal) and N-acetyl galactosamine (GalNAc) were highly expressed in primary benign meningioma cells (WHO grade I) compared to malignant meningioma cell lines (WHO grade III). The expression profile of mucin types O-glycosyltransferases in meningiomas were observed through the GEO database and gene expression experiment in meningioma cell lines. In the GEO database, C1GALT1-specific chaperone (COSMC) and mucin 1 (MUC1) were significantly increased in malignant meningiomas (Grade II and III) compared with benign meningiomas (Grade I). Meanwhile, in the cell lines, Core 2 β1,6-N-acetylglucosaminyltransferase-2 (C2GNT2) was highly expressed in malignant meningiomas. We then investigated the complex mucin-type O-glycans structures by determination of sialyltransferases and fucosyltransferases. We found ST3 β-galactoside α-2,3-sialyltransferase 4 (ST3GAL4) was significantly decreased in the GEO database, while ST3GAL1, ST3GAL3, α1,3 fucosyltransferases 1 and 8 (FUT1 and FUT8) were highly expressed in malignant meningioma cell lines—(HKBMM)—compared to primary benign meningioma cells—(SUT-MG12 and SUT-MG14).

Conclusion
Our findings are the first to demonstrate the potential glycosylation changes in the O-linked glycans of malignant meningiomas compared with benign meningiomas, which may play an essential role in the progression, tumorigenesis, and malignancy of meningiomas.
Current State and Future Potential of Fisheries in the Mirsharai Coastal Areas of Chattogram for Enhancing Bangladesh’s Blue Economy
Authors: Mondal Md. Atiqul Islam , Siddiqui Abdullah Al Mamun , Rani Seema , Chowdhury Showmitra , Chowdhury Siraj Uddin Md. Babar 
Published: January 2024

Aims: This study aims to comprehensively assess the fisheries resources in the Mirsharai coastal area of Chattogram district, focusing on their contribution to both local and national economies. The research seeks to identify challenges and vulnerabilities, particularly those arising from climate change, impacting pond owners, fish farmers, hatchery owners, and nursery owners in the region.
Study Design: The study adopts a cross-sectional design, employing field surveys and interviews to collect data on fish production, and challenges faced by stakeholders. The cross-sectional approach allows for a holistic understanding of the current state of fisheries resources and their interactions with climatic factors.
Place and Duration of Study: The research was conducted in Mirsharai, a coastal upazila of Chattogram district, Bangladesh. The study encompasses data collected during the January to December 2016, providing insights into the dynamics of fish production, and challenges faced by fisheries stakeholders in Mirsharai.
Methodology: Field surveys were conducted to gather quantitative data on fish production from both capture and culture fisheries. Additionally, interviews with pond owners, fish farmers, hatchery owners, and nursery owners were conducted to qualitatively assess the impact of challenges faced by fisheries stakeholders in Mirsharai on their operations and economic well-being. The combination of quantitative and qualitative methods ensures a comprehensive analysis.
Results: The average fish production in capture fisheries was determined to be 63 kg/ha, while culture fisheries, specifically pond aquaculture, yield an average of 1656 kg/ha. The results highlight the significant impact of challenges faced by fisheries stakeholders in Mirsharai on the local fisheries sector, revealing challenges related to market timing, post-larvae availability, and pricing that affect the economic sustainability of stakeholders.
Conclusion: The findings underscore the vulnerability of Mirsharai's fisheries to challenges faced by fisheries stakeholders in Mirsharai, emphasizing economic losses for fish farmers, hatchery owners, and nursery owners. The conclusion calls for urgent adaptive measures and climate-resilient strategies in the fisheries sector to ensure sustainability and viability in the face of evolving climatic conditions.
Implication: This study has implications for fisheries management and policy development, emphasizing the need for proactive measures to address climate-induced challenges and sustain the economic well-being of fisheries stakeholders in the Mirsharai coastal area.
Effects of Disease Complex of Penicellium and Root Knot Nematode on The growth and Development of Chilli (Capsicum annum L.) Crop
Authors: Samanta Sanjoy , Shelar Vaibhav Balasaheb , Banerjee Santanu , Doggalli Gangadhara , Hazarika Sanjay , Agrawal Smita , Singh Shivam , Shahni Yumkhaibam Sonia 
Published: January 2024

The behaviour of fungal pathogens in a dormant or active state under different changes by infection of the host plant parasitic nematode was related to the function of fungi. Due to RKNs on the chilli in Meloidogyneinfected pot culture, Penicillium sp. demonstrated plant growth-promoting characteristics and nematocidal activities, according to the findings of the current study. This fungus can be used as a growth promoter in Treatments 2 and 3, where a mixture of Meloidogyne sp. and Penicelliumsp. showed higher growth rates and yellowing leaves in some pots, but no knots were found in the roots however, the current research may offer a theoretical basis for a growth promoter agent for better growth in Penicillium inoculated pots and In Nematode inoculated pots, Meloidogynesp can reduce plant growth. In both Meliodogynesp and Penicillium sp inoculated pots, it was demonstrated that decreasing nematode population increased the growth habits of chilies in terms of number of leaves Therefore, it has been demonstrated that Penicellium decreases nematocidal action while promoting growth parameters in chilli plants, and that using preventative measures can somewhat lessen insect infestation in control pots.
Glutamine Synthetase GlnA1 Is Essential for Growth of
Mycobacterium tuberculosis
in Human THP-1 Macrophages and Guinea Pigs
Authors: Tullius Michael V. , Harth Günter , Horwitz Marcus A. 
Published: June 2003

ABSTRACT

To assess the role of glutamine synthetase (GS), an enzyme of central importance in nitrogen metabolism, in the pathogenicity of
Mycobacterium tuberculosis
, we constructed a
glnA1
mutant via allelic exchange. The mutant had no detectable GS protein or GS activity and was auxotrophic for
l
-glutamine. In addition, the mutant was attenuated for intracellular growth in human THP-1 macrophages and avirulent in the highly susceptible guinea pig model of pulmonary tuberculosis. Based on growth rates of the mutant in the presence of various concentrations of
l
-glutamine, the effective concentration of
l
-glutamine in the
M
.
tuberculosis
phagosome of THP-1 cells was ∼10% of the level assayed in the cytoplasm of these cells (4.5 mM), indicating that the
M
.
tuberculosis
phagosome is impermeable to even very small molecules in the macrophage cytoplasm. When complemented by the
M
.
tuberculosis glnA1
gene, the mutant exhibited a wild-type phenotype in broth culture and in human macrophages, and it was virulent in guinea pigs. When complemented by the
Salmonella enterica
serovar Typhimurium
glnA
gene, the mutant had only 1% of the GS activity of the
M
.
tuberculosis
wild-type strain because of poor expression of the
S
.
enterica
serovar Typhimurium GS in the heterologous
M
.
tuberculosis
host. Nevertheless, the strain complemented with
S
.
enterica
serovar Typhimurium GS grew as well as the wild-type strain in broth culture and in human macrophages. This strain was virulent in guinea pigs, although somewhat less so than the wild-type. These studies demonstrate that
glnA1
is essential for
M
.
tuberculosis
virulence.
Deletion of a
Vibrio cholerae
ClC Channel Results in Acid Sensitivity and Enhanced Intestinal Colonization
Authors: Ding Yanpeng , Waldor Matthew K. 
Published: June 2003

ABSTRACT

ClC chloride channels are found in all three kingdoms of life though little is known about their functions in prokaryotes. Here we investigated the role of a
Vibrio cholerae
ClC channel in acid resistance and intestinal colonization. The putative
V. cholerae
ClC channel was found to confer mild resistance to acid when pH was adjusted with HCl, but not with other acids. Surprisingly, a ClC channel deletion mutant exhibited enhanced intestinal colonization in infant mice.
Mutation of
sarA
in
Staphylococcus aureus
Limits Biofilm Formation
Authors: Beenken Karen E. , Blevins Jon S. , Smeltzer Mark S. 
Published: June 2003

ABSTRACT

Mutation of
sarA
resulted in a reduced capacity to form a biofilm in six of the eight
Staphylococcus aureus
strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation of
agr
. Mutation of
agr
in other strains had little impact on biofilm formation. In every strain other than Newman, including RN6390, simultaneous mutation of
sarA
and
agr
resulted in a phenotype like that observed with the
sarA
mutants. Complementation studies using a
sarA
clone confirmed that the defect in biofilm formation was due to the
sarA
mutation.
Interruptibility of long call production in tamarins: implications for vocal control
Authors: Miller Cory T. , Flusberg Stephen , Hauser Marc D. 
Published: June 2003

SUMMARYVocal production can be highly deterministic, such that once the central nervous system generates a signal to call, the vocalization is emitted immune to external events. Conversely, vocal production can be modulated by auditory feedback such that interference or disruption can cause an individual to stop calling or, if it continues to call, for the acoustic morphology of the signal to change. To explore which of these models best accounts for the control of vocal production in non-human primates, we adapted an interruption technique originally developed for songbirds for use with a New World monkey species,the cotton-top tamarin (Saguinus oedipus). Results from a pilot experiment indicated that an auditory stimulus (white noise) was more effective than a visual stimulus (strobe light) at interrupting the tamarin's species-typical `combination long call (CLC)'. Data from a second experiment showed that although the duration of the auditory stimulus did not affect the proportion of interruptions that occurred, a 1000 ms white noise stimulus perturbed the temporal structure of the CLC to a greater extent than did a 250 ms white noise stimulus. Furthermore, when call production was interrupted,tamarins stopped vocalizing after the completion of a syllable, suggesting that the syllable represents a unit of organization within the call. Overall,these results provide evidence that tamarins can modify their vocal output based on external events, but the degree of vocal control is significantly less than in oscine songbirds.
A Study of the Relative Responsiveness of Five Sensibility Tests for Assessment of Recovery after Median Nerve Injury and Repair
Authors: JEROSCH-HEROLD CHRISTINA 
Published: June 2003

A longitudinal dynamic cohort study was conducted on patients with median nerve injuries to evaluate the relative responsiveness of five sensibility tests: touch threshold using the WEST (monofilaments), static two-point discrimination, locognosia, a pick-up test and an object recognition test. Repeated assessments were performed starting at 6 months after surgery. In order to compare the relative responsiveness of each test, effect size and the standard response mean were calculated for sensibility changes occurring between 6 and 18 months after repair. Large effect sizes (>0.8) and standard response means (>0.8) were obtained for the WEST, locognosia, pick-up and object recognition tests. Two-point discrimination was hardly measurable at any time point and exhibited strong flooring effects. Further analysis of all time points was undertaken to assess the strength of the monotonic relationship between test scores and time elapsed since surgery. Comparison of monotonicity between the five tests indicated that the WEST performed best, whereas two-point discrimination performed worst. These results suggest that the monofilament test (WEST), locognosia test, Moberg pick-up test and tactile gnosis test capture sensibility changes over time well and should be considered for inclusion in the outcome assessment of patients with median nerve injury.
Nitric Oxide Production and Mononuclear Cell Nitric Oxide Synthase Activity in Malaria-Tolerant Papuan Adults
Authors: Boutlis Craig S. , Tjitra Emiliana , Maniboey Helena , Misukonis Mary A. , Saunders Jocelyn R. , Suprianto Sri , Weinberg J. Brice , Anstey Nicholas M. 
Published: June 2003

ABSTRACTIndividuals living in regions of intense malaria transmission exhibit natural immunity that allows them to be without fever and other symptoms for most of the time despite frequent parasitization. Although this tolerance of parasitemia appears to be more effective in children than in adults (as evidenced by lower parasitemia fever thresholds with age), adults do exhibit a degree of tolerance but the mechanism(s) underlying this are unclear. Asymptomatic malaria-exposed children have higher levels of nitric oxide (NO) than children with severe disease, and NO has been proposed as a mediator of malarial tolerance. However, the ability of highly malaria-exposed asymptomatic adults to generate high-level basal NO is unknown, as is the relationship between NO and malaria tolerance in adults. The relationship between NO and malaria parasitemia was therefore determined in asymptomatic adults from Papua, Indonesia. Adults withPlasmodium falciparumparasitemia had markedly increased basal systemic NO production relative to aparasitemic Papuan controls, who in turn produced more NO than healthy controls from a region without malaria. Immunoglobulin E levels were universally elevated in malaria-exposed Papuan subjects, suggesting that the prevalence of intestinal parasitosis may be high and that nonmalarial infection may also contribute to high basal NO production. Basal peripheral blood mononuclear cell (PBMC) NO synthase activity was elevated in Papuans but poorly correlated with systemic NO production, suggesting that NO production in this setting arises not only from PBMCs but also from other tissue and cellular sources. NO production was associated with and may contribute to malaria tolerance in Papuan adults.
Overexpression of Interleukin-4 in Lungs of Mice Impairs Elimination ofHistoplasma capsulatum
Authors: Gildea Lucy A. , Gibbons Reta , Finkelman Fred D. , Deepe George S. 
Published: June 2003

ABSTRACTProtection against the pathogenic fungusHistoplasma capsulatumrequires Th1 cytokines. Since interleukin-4 (IL-4) can inhibit both Th1 cytokine production and activity, we examined the effects of overproduction of IL-4 in the lung on the course of pulmonary histoplasmosis. IL-4 lung transgenic mice manifested a higher fungal burden in their lungs, but not spleens, compared to wild-type infected controls. Despite the higher burden, the transgenic animals were ultimately capable of controlling infection. The adverse effects of IL-4 onH. capsulatumelimination were not observed during the early phase of infection (days 1 to 3) but were maximal at day 7 postinfection, prior to the induction of cell-mediated immunity. Analysis of total body and lung cytokine levels revealed that gamma interferon and tumor necrosis factor alpha production were not inhibited in the presence of excess IL-4. Our results with transgenic mice were supported by additional in vivo studies in which allergen induction of pulmonary IL-4 was associated with delayed clearance ofH. capsulatumyeast and increased fungal burden. These findings demonstrate that excess production of endogenous IL-4 modulates protective immunity toH. capsulatumby delaying clearance of the organism but does not prevent the generation of a Th1 response that ultimately controls infection.
The Largest Open Reading Frame (
pks12
) in the
Mycobacterium tuberculosis
Genome Is Involved in Pathogenesis and Dimycocerosyl Phthiocerol Synthesis
Authors: Sirakova Tatiana D. , Dubey Vinod S. , Kim Hwa-Jung , Cynamon Michael H. , Kolattukudy Pappachan E. 
Published: June 2003

ABSTRACT

The cell wall lipids in
Mycobacterium tuberculosis
are probably involved in pathogenesis. The largest open reading frame in the genome of
M. tuberculosis
H37Rv,
pks12
, is unique in that it encodes two sets of domains needed to produce fatty acids. A
pks12
-disrupted mutant was produced, and disruption was confirmed by both PCR analysis and Southern blotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that a 430-kDa protein band present in the wild type was missing in the mutant. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) and liquid chromatography (LC)-MS analysis of tryptic peptides showed that 54 peptides distributed throughout this protein matched the
pks12-
encoded sequence. Biochemical analysis using [1-
14
C]propionate as the radiotracer showed that the
pks12
mutant was deficient in the synthesis of dimycocerosyl phthiocerol (DIM). SDS-PAGE, immunoblot analysis of proteins, and analysis of fatty acids showed that the mutant can produce mycocerosic acids. Thus, the
pks12
gene is probably involved in the synthesis of phthiocerol, the diol required for DIM synthesis. Growth of the
pks12
mutant was attenuated in mouse alveolar macrophage cell line MH-S, and the virulence of the mutant in vivo was highly attenuated in a murine model. Thus,
pks12
probably participates in DIM production and its expression is involved in pathogenesis.
The C-Terminal Domain of
Salmonella enterica
Serovar Typhimurium OmpA Is an Immunodominant Antigen in Mice but Appears To Be Only Partially Exposed on the Bacterial Cell Surface
Authors: Singh Shiva P. , Williams Yvonne U. , Miller Stephanie , Nikaido Hiroshi 
Published: June 2003

ABSTRACT

We examined the way the major outer membrane protein OmpA of
Salmonella enterica
serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs) against this protein. Interaction between OmpA and these MAbs is competitively inhibited with several-hundredfold dilutions of mouse polyclonal sera obtained by immunization with live or heat-killed whole cells, suggesting that OmpA is one of the immunodominant antigens of serovar Typhimurium. All of the MAbs were specific for an identical epitope(s) located on the C-terminal domain of OmpA, as indicated by the use of OmpA fragments generated by protease or cyanogen bromide treatment and by competitive inhibition enzyme-linked immunosorbent assay. This epitope was highly conserved within (but not outside) the family
Enterobacteriaceae
. The strong immunogenicity of this epitope was surprising because the C-terminal domain of OmpA, usually thought to be located in the periplasm, is not expected to be exposed on the bacterial cell surface. A MAb, however, reacted in a cytofluorometry assay more strongly with outer-membrane-permeabilized cells than with untreated cells, a result supporting the predominantly periplasmic localization of the epitope. Significant, though low-level, reactivity of intact cells nevertheless suggests that in some cells the C-terminal domain of OmpA is exposed on the surface, a result consistent with the proposal that OmpA can fold into one of the two alternate conformations.