bluuebunny/crossref_metadata_2025_split huggingface dataset:
Economy-wide impact of climate smart agriculture in India: a SAM framework
Authors: Ajatasatru Ananya , Prabhu Vishnu , Pal Barun Deb , Mukhopadhyay Kakali 
Published: January 2024

AbstractIn the context of climate change, the Indian agricultural sector treads in a certain duality between promoting food security in response to the increasing population, but at the same time in ensuring environmental sustainability, and sustained economic growth, especially in developing countries like India. The concept of Climate Smart Agriculture (CSA) emerged from the recognition of this duality. Using the Indian Social Accounting Matrix (SAM) 2017–18, the economy-wide effects arising out of agricultural interventions were estimated, keeping accord with the impacts on sectoral outputs and household incomes from the adoption of varying CSA interventions such as Conservation Agriculture, System of Rice and Wheat Intensification (SRI-SWI) and Natural Farming, fitting the three-pillared criterion of CSA—(1) Productivity (2) Adaptation and (3) Mitigation. Additionally, a shift in cropping patterns from Paddy and Wheat to less emission-intensive crops was also studied. Results show that SRI-SWI provides the highest economy-wide impacts while accounting for lower GHG and water footprint. Alternative crops such as Maize, Sorghum, and Millet have minimal increase in income and output effects while having lower water and carbon intensity compared to rice and wheat. The current study would sensitize policymakers to prioritize suitable policy and institutional measures for upscaling climate smart interventions in India.
Modelling the nexus between finance, government revenue, institutional quality and sustainable energy supply in West Africa
Authors: Kolawole Kayode David , Abdulmumin Biliqees Ayoola , Uzuner Gizem , Seyingbo Oluwagbenga Abayomi , Abdulrauf Lukman Adebayo-Oke 
Published: January 2024

AbstractThe present study examined the relationship between finance, government revenue, institutional quality and sustainable energy supply in West Africa countries over annual frequency period from 2012 to 2020. To achieve the outlined nexus between study variables, the present study leverages on a battery of panel analysis for robust inferences. The econometric estimators employed are panel random effect regression, generalized method of moment technique. Furthermore, panel Granger causality test is utilized to analyze the direction of flow among the variables for the study. Empirical results revealed that financial development is a significant determinant of energy supply in West Africa countries while a negligible effect was reported for institutional quality and sustainable energy supply. Thus, the present study concludes that finance from financial sector is important in ensuring sufficient energy supply. To this end, this study therefore, recommends that incentives should be given to financial institutions that fund energy generation and transmission as financial development is seen to be significant on energy supply.
Nanotechnology for microglial targeting and inhibition of neuroinflammation underlying Alzheimer’s pathology
Authors: Gebril Hoda M. , Aryasomayajula Aravind , de Lima Mariana Reis Nogueira , Uhrich Kathryn E. , Moghe Prabhas V. 
Published: January 2024

Abstract
Background
Alzheimer's disease (AD) is considered to have a multifactorial etiology. The hallmark of AD is progressive neurodegeneration, which is characterized by the deepening loss of memory and a high mortality rate in the elderly. The neurodegeneration in AD is believed to be exacerbated following the intercoupled cascades of extracellular amyloid beta (Aβ) plaques, uncontrolled microglial activation, and neuroinflammation. Current therapies for AD are mostly designed to target the symptoms, with limited ability to address the mechanistic triggers for the disease. In this study, we report a novel nanotechnology based on microglial scavenger receptor (SR)-targeting amphiphilic nanoparticles (NPs) for the convergent alleviation of fibril Aβ (fAβ) burden, microglial modulation, and neuroprotection.

Methods
We designed a nanotechnology approach to regulate the SR-mediated intracellular fAβ trafficking within microglia. We synthesized SR-targeting sugar-based amphiphilic macromolecules (AM) and used them as a bioactive shell to fabricate serum-stable AM–NPs via flash nanoprecipitation. Using electron microscopy, in vitro approaches, ELISA, and confocal microscopy, we investigated the effect of AM–NPs on Aβ fibrilization, fAβ-mediated microglial inflammation, and neurotoxicity in BV2 microglia and SH-SY5Y neuroblastoma cell lines.

Results
AM–NPs interrupted Aβ fibrilization, attenuated fAβ microglial internalization via targeting the fAβ-specific SRs, arrested the fAβ-mediated microglial activation and pro-inflammatory response, and accelerated lysosomal degradation of intracellular fAβ. Moreover, AM–NPs counteracted the microglial-mediated neurotoxicity after exposure to fAβ.

Conclusions
The AM–NP nanotechnology presents a multifactorial strategy to target pathological Aβ aggregation and arrest the fAβ-mediated pathological progression in microglia and neurons.

Graphical Abstract
Effects of combined nutrient and pesticide exposure on algal biomass, and Daphnia magna abundance
Authors: Onyango Joel , van Bruggen J. J. A. , Kitaka Nzula , Simaika John , Irvine Kenneth 
Published: January 2024

AbstractFertilisers and pesticides are increasingly used in agriculture to improve productivity and protect crops from fungi and insects. However, these farm inputs may lead to adverse effects on aquatic biodiversity through eutrophication and pesticide toxicity. This study aimed to establish the effects of nutrient-only, pesticide-only, combined nutrients and pesticides, and control on the abundance of Daphnia magna, and algal biomass. In each of the treatments, different concentrations of nutrients and pesticides residues were added separately or in combination. Responses were measured every 24 h, and the experiments ended after 168 h of exposure. The experiment was set in four concentration treatments comprising high, moderately high, moderately low, and low concentrations. Data analysis was done using Multiple Analysis of Variance (MANOVA) and ANOVA to determine the effect of time, concentrations and the interaction of time and concentrations for each of the treatments on D. magna abundance, and algal biomass. Higher concentrations of pesticide additives were associated with lower abundance of D. magna, and higher algal biomass over the exposure periods. There was a significant reduction in the abundance of D. magna in the combined treatment indicating the toxic effect of pesticide addition. Determination of effect concentrations based on combined nutrients-pesticides experiments becomes important in setting water quality standards, and monitoring the quality status, to avoid underestimating the ecological implications of combined contamination.
Acellular scaffold-based approach for in situ genetic engineering of host T-cells in solid tumor immunotherapy
Authors: Dandia Hiren Y. , Pillai Mamatha M. , Sharma Deepak , Suvarna Meghna , Dalal Neha , Madhok Ayush , Ingle Arvind , Chiplunkar Shubhada V. , Galande Sanjeev , Tayalia Prakriti 
Published: January 2024

Abstract
Background
Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological malignancies. However, its application to solid tumors presents significant challenges due to the limited accessibility and heterogeneity. Localized delivery of tumor-specific T-cells using biomaterials has shown promise, however, procedures required for genetic modification and generation of a sufficient number of tumor-specific T-cells ex vivo remain major obstacles due to cost and time constraints.

Methods
Polyethylene glycol (PEG)-based three-dimensional (3D) scaffolds were developed and conjugated with positively charged poly-L-lysine (PLL) using carbamide chemistry for efficient loading of lentiviruses (LVs) carrying tumor antigen-specific T-cell receptors (TCRs). The physical and biological properties of the scaffold were extensively characterized. Further, the scaffold loaded with OVA-TCR LVs was implanted in B16F10 cells expressing ovalbumin (B16-OVA) tumor model to evaluate the anti-tumor response and the presence of transduced T-cells.

Results
Our findings demonstrate that the scaffolds do not induce any systemic inflammation upon subcutaneous implantation and effectively recruit T-cells to the site. In B16-OVA melanoma tumor-bearing mice, the scaffolds efficiently transduce host T-cells with OVA-specific TCRs. These genetically modified T-cells exhibit homing capability towards the tumor and secondary lymphoid organs, resulting in a significant reduction of tumor size and systemic increase in anti-tumor cytokines. Immune cell profiling revealed a significantly high percentage of transduced T-cells and a notable reduction in suppressor immune cells within the tumors of mice implanted with these scaffolds.

Conclusion
Our scaffold-based T-cell therapy presents an innovative in situ localized approach for programming T-cells to target solid tumors. This approach offers a viable alternative to in vitro manipulation of T-cells, circumventing the need for large-scale in vitro generation and culture of tumor-specific T-cells. It offers an off-the-shelf alternative that facilitates the use of host cells instead of allogeneic cells, thereby, overcoming a major hurdle.
Selective regulation of endophytic bacteria and gene expression in soybean by water-soluble humic materials
Authors: Wang Wenqian , Li Dongmei , Qiu Xiaoqian , Yang Jinshui , Liu Liang , Wang Entao , Yuan Hongli 
Published: January 2024

Abstract
Background
As part of the plant microbiome, endophytic bacteria play an essential role in plant growth and resistance to stress. Water-soluble humic materials (WSHM) is widely used in sustainable agriculture as a natural and non-polluting plant growth regulator to promote the growth of plants and beneficial bacteria. However, the mechanisms of WSHM to promote plant growth and the evidence for commensal endophytic bacteria interaction with their host remain largely unknown. Here, 16S rRNA gene sequencing, transcriptomic analysis, and culture-based methods were used to reveal the underlying mechanisms.

Results
WSHM reduced the alpha diversity of soybean endophytic bacteria, but increased the bacterial interactions and further selectively enriched the potentially beneficial bacteria. Meanwhile, WSHM regulated the expression of various genes related to the MAPK signaling pathway, plant-pathogen interaction, hormone signal transduction, and synthetic pathways in soybean root. Omics integration analysis showed that Sphingobium was the genus closest to the significantly changed genes in WSHM treatment. The inoculation of endophytic Sphingobium sp. TBBS4 isolated from soybean significantly improved soybean nodulation and growth by increasing della gene expression and reducing ethylene release.

Conclusion
All the results revealed that WSHM promotes soybean nodulation and growth by selectively regulating soybean gene expression and regulating the endophytic bacterial community, Sphingobium was the key bacterium involved in plant-microbe interaction. These findings refined our understanding of the mechanism of WSHM promoting soybean nodulation and growth and provided novel evidence for plant-endophyte interaction.
Dietary preferences and quality of life among dialysis patients in Pune: a cross-sectional study
Authors: Gokhale Devaki , Kaskar Sehlaa , Bansal Ariti 
Published: January 2024

Abstract
Background
To assess the dietary preference and quality of life among dialysis patients in Pune.

Method
This cross-sectional study was conducted among 127 dialysis patients aged 18–70 through a one-on-one interview to record data on demographic, biochemical, diet preference, and quality of life. The anthropometric and biochemical parameters were recorded with the help of patient reports from the four dialysis centers. A kidney disease quality of life questionnaire was used to assess the quality of life.

Result
The mean age of the dialysis patients was (49.1 ± 12.9), comprising of males (104, 81.9%). (63, 49.6%) of the participants belonged to the normal weight category, followed by the underweight category. No association was noted between diet preference and quality of life, but a significant difference between BMI and personal appearance was observed. Serum calcium levels were highest (64.29 ± 1.0) in individuals in the high category of the effect of kidney disease compared to low serum calcium levels (20.89 ± 14.71) in the low category of the same.

Conclusion
The present study found that diet preferences were affected due to diet restrictions, but their association with quality of life was not significantly proven. The mean for the disease burden was the lowest, implying poor quality of life among dialysis patients. Therefore, providing nutrition education and counseling for dialysis patients is crucial. A detailed dialysis care plan must address all patient requirements, including medication, dietary changes, modifications, and malnutrition screenings.
Relationship between tobacco smoking and hematological indices among Sudanese smokers
Authors: Ahmed Izzut Awad , Mohammed Mahmood Abdalmonem , Hassan Hussam Mohammed , Ali Ibrahim Abdelrhim 
Published: January 2024

Abstract
Background
Tobacco Smoking is one of the leading causes of preventable morbidity and mortality in the world. It is well documented that tobacco smoking is risk factor for many diseases like: cancers, chronic respiratory and cardiovascular diseases, and the effects of tobacco smoking on hematological indices gets a little attention: the data is mostly inconsistent regarding the differential of WBCs, a conflicting studies described the effect of smoking on hemoglobin descriptive parameters and a regular monitoring of platelets count in smokers was advised.

Objectives
The study aimed to evaluate the relationship between tobacco smoking and hematological parameters among Sudanese healthy Smokers at Bahri Town.

Methods
This was a cross sectional study conducted during 2022 in Bahri town, Khartoum state. A total of 120 male subjects participated in this study. Of them, 60 healthy non-smokers participants (Control), and 60 age matched smokers who were smoking tobacco for a minimum of 1 year. Smokers group was divided into three major sub-groups with each group contains 20 subjects: Cigarettes smokers (CS), Water pipes (Shisha) smokers (WP) and both Cigarettes and water pipes (shisha) smokers (CSWP). Data was collected through questionnaire interviews and laboratory investigation. A sample of Five ml venous blood was taken for Complete blood count testing using Urite 3000 plus semi-automated hematology analyzer. Data were analyzed using SPSS version 25. Assocation between the variables were estimated and p value ≤ 0.05 was considered statistically significant.

Results
Smokers had significantly higher RBCs count (p = 0.017), Hb level (p < 0.001), WBCs count (p = .017), Neutrophils (p < 0.001), MCH (p = 0.029), MCHC (p < 0.001), RDW (p < 0.001), and PDW (p < 0.001) compared to the non-smokers. In contrast, non-smokers had higher MPV (p < 0.001) and MCV (p < 0.001) levels than smokers. Between the non-smokers and different subtypes of the smokers (CS, WP & CSWP), there were significant differences between the subgroups for all hematological parameters except for PLTs and lymphocytes count. CS had lower levels of MCV (p < 0.001), MCHC (p < 0.001), HCT (p = 0.036), and RDW (p < 0.001) compared to the non-smokers, while both cigarette and shisha smokers had the higher levels of neutrophils count (p < 0.001) and PDW (p < 0.001) compared to the non-smokers.

Conclusion
Smoking affects hematological parameters; smokers had significantly higher RBCs count, Hb level, WBCs count, Neutrophils, MCH, MCHC, RDW and PDW compared to the non-smoker group. WP smoking caused higher levels of RBCs, Hb, neutrophils, MCH and MCHC. PDW was high in smokers’ sub-groups compared to control group, while MPV was lower despite insignificant change In PLTs count.
Building a resilient health system for universal health coverage and health security: a systematic review
Authors: Debie Ayal , Nigusie Adane , Gedle Dereje , Khatri Resham B. , Assefa Yibeltal 
Published: January 2024

Abstract
Background
Resilient health system (RHS) is crucial to achieving universal health coverage (UHC) and health security. However, little is known about strategies towards RHS to improve UHC and health security. This systematic review aims to synthesise the literature to understand approaches to build RHS toward UHC and health security.

Methods
A systematic search was conducted including studies published from 01 January 2000 to 31 December 2021. Studies were searched in three databases (PubMed, Embase, and Scopus) using search terms under four domains: resilience, health system, universal health coverage, and health security. We critically appraised articles using Rees and colleagues’ quality appraisal checklist to assess the quality of papers. A systematic narrative synthesis was conducted to analyse and synthesise the data using the World Health Organization’s health systems building block framework.

Results
A total of 57 articles were included in the final review. Context-based redistribution of health workers, task-shifting policy, and results-based health financing policy helped to build RHS. High political commitment, community-based response planning, and multi-sectorial collaboration were critical to realising UHC and health security. On the contrary, lack of access, non-responsive, inequitable healthcare services, poor surveillance, weak leadership, and income inequalities were the constraints to achieving UHC and health security. In addition, the lack of basic healthcare infrastructures, inadequately skilled health workforces, absence of clear government policy, lack of clarity of stakeholder roles, and uneven distribution of health facilities and health workers were the challenges to achieving UHC and health security.

Conclusions
Advanced healthcare infrastructures and adequate number of healthcare workers are essential to achieving UHC and health security. However, they are not alone adequate to protect the health system from potential failure. Context-specific redistribution of health workers, task-shifting, result-based health financing policies, and integrated and multi-sectoral approaches, based on the principles of primary health care, are necessary for building RHS toward UHC and health security.
Test–retest reliability of the FitMáx©-questionnaire in a clinical and healthy population
Authors: Meijer Renske , Schep Goof , Regis Marta , Papen-Botterhuis Nicole E. , Savelberg Hans H. C. M. , van Hooff Martijn 
Published: January 2024

Abstract
Purpose
The FitMáx© was developed as a questionnaire-based instrument to estimate Cardiorespiratory Fitness (CRF) expressed as oxygen uptake at peak exercise (VO2peak). Test–retest reliability is a clinometric measurement property, which defines stability over time if multiple measurements are performed (i.e. reliability). The present study aimed to assess the test–retest reliability of the FitMáx©-questionnaire in different patient groups.

Patients and methods
A total of 127 cardiac, pulmonary and oncology patients and healthy subjects aged 19–84 years who completed the questionnaire twice within an average of 18 days were included for analysis. Participants were in a stable clinical situation (no acute disease or participating in a training program). To determine the test–retest reliability, the Intraclass Correlation Coefficient (ICC) and Standard Error of the Measurement (SEM) was calculated between the first (T0) and second (T1) administration of the questionnaires.

Results
An excellent agreement was found between the FitMáx©-questionnaire scores at T0 and T1, with an ICC of 0.97 (SEM 1.91) in the total study population and an ICC ranging from 0.93 to 0.98 (SEM 1.52–2.27) in the individual patient groups.

Conclusion
The FitMáx©-questionnaire proves to be reliable and stable over time to estimate CRF of patients and healthy subjects.
Trial registration NTR (Netherlands Trial Register), NL8846. Registered 25 August 2020, https://trialsearch.who.int/Trial2.aspx?TrialID=NL8846
Cardiac-induced motion of the pancreas and its effect on image quality of ultrahigh-resolution CT
Authors: Holmes Thomas Wesley , Yu Zhou , Thompson Richard , Oshinski John N. , Pourmorteza Amir 
Published: January 2024

AbstractRecent advancements in diagnostic CT detector technology have made it possible to resolve anatomical features smaller than 20 LP/cm, referred to as ultra-high-resolution (UHR) CT. Subtle biological motions that did not affect standard-resolution (SR) CT may not be neglected in UHR. This study aimed to quantify the cardiac-induced motion of the pancreas and simulate its impact on the image quality of UHR-CT. We measured the displacement of the head of the pancreas in three healthy volunteers using Displacement Encoding with Stimulated Echoes (DENSE) MRI. The results were used to simulate SR- and UHR-CT acquisitions affected by pancreatic motion.We found pancreatic displacement in the 0.24–1.59 mm range during one cardiac cycle across the subjects. The greatest displacement was observed in the anterior–posterior direction. The time to peak displacement varied across subjects. Both SR and UHR images showed reduced image quality, as measured by radial modulation transfer function, due to cardiac-induced motion, but the motion artifacts caused more severe degradation in UHR acquisitions. Our investigation of cardiac-induced pancreatic displacement reveals its potential to degrade both standard and UHR-CT scans. To fully utilize the improvement in spatial resolution offered by UHR-CT, the effects of cardiac-induced motion in the abdomen need to be understood and corrected.Relevance statement Advancements in CT detector technology have enhanced CT scanner spatial resolution to approximately 100 µm. Consequently, previously ignored biological motions such as the cardiac-induced motion of the pancreas now demand attention to fully utilize this improved resolution.
Graphical Abstract
Molecular characterization and biocontrol potential of Trichoderma spp. against Fusarium oxysporum f. sp. dianthi in carnation
Authors: Zandyavari Nazyar , Sulaiman Mueed Ali , Hassanzadeh Nader 
Published: January 2024

AbstractBackgroundCarnation, a major cut flower product cultivated economically in Iran, faces economic challenges due to the devastatingFusariumwilt caused byFusarium oxysporumf. sp.dianthi(Fod). To address this issue, twenty-fiveTrichoderma harzianumandT. virideisolates were collected from the rhizosphere soil of three Iranian provinces: Tehran, Markazi, and Fars. RAPD-PCR was applied to analyze the genetic relatedness of the isolates.ResultsThe RAPD profiles showed genetic diversity among the isolates, with two major clusters. The antagonistic potential of the twenty isolates was evaluated against the carnation wilt caused byFod. The results showed that Th1, Th7, and Th2 isolates ofT. harzianumsignificantly inhibitedFodmycelial growth (58, 56.5, and 48.6%, respectively). AmongT. virideisolates, Tv5, Tv4, and Tv7 exhibited the highest antifungal ability to inhibit mycelial growth. All investigated isolates ofTrichodermasecreted volatile compounds that hinderedFodmycelial growth, with isolates ofT. harzianumranging from 9.3 to 67.5% inhibition and those ofT. viridefrom 25.2 to 50.2%. Additionally, the experiment on competitive saprophytic ability indicated that maximum colonization occurred with Th1, Th7, and Th2 isolates at 78.2, 70.8, and 69.8%, respectively. Lastly, the greenhouse experiment showed a complete pathogen eradication or significant inhibition in the infected carnation afterT. harzianumandT. viride. Conversely, control treatment with theFodpathogen died after 90 days.ConclusionsThe investigation suggested thatTrichodermaspp. could be a potential biocontrol agent to mitigateFusariumwilt in carnation and improve production quality, replacing chemical pesticides.