bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive characterization of adipogenesis-related genes in colorectal cancer for clinical significance and immunogenomic landscape analyses
Authors: Han Jing , Li Shangshang , Zhan Qiong , Hu Yuchao , Zhong Chaoxiang , Yang Jie , Qiu Zhengcai 
Published: December 2023

Abstract
Objective
Colorectal cancer (CRC) is a major global health concern, necessitating the identification of biomarkers and molecular subtypes for improved clinical management. This study aims to evaluate the clinical value of adipogenesis-related genes and molecular subtypes in CRC.

Methods
A comprehensive analysis of adipogenesis-related genes in CRC was performed using publicly available datasets (TCGA and GEO database) and bioinformatics tools. Unsupervised cluster analysis was employed to identify the molecular subtypes of CRC, while LASSO regression analysis was utilized to develop a risk prognostic model. The immunogenomic patterns and immunotherapy analysis were used to predict patient response to immunotherapy. Furthermore, qPCR analysis was conducted to confirm the expression of the identified key genes in vitro.

Results
Through the analysis of RNAseq data from normal and tumor tissues, we identified 50 differentially expressed genes. Unsupervised cluster analysis identified two subtypes (Cluster A and Cluster B) with significantly different survival outcomes. Cluster A and B displayed differential immune cell compositions and enrichment in specific biological pathways, providing insights into potential therapeutic targets. A risk-scoring model was developed using five ARGs, which successfully classified patients into high and low-risk groups, showing distinct survival outcomes. The model was validated and showed robust predictive performance. High-risk patients exhibited altered immune cell proportions and gene expression patterns compared to low-risk patients. In qPCR validation, four out of the five key genes were consistent with the results of bioinformatics analysis.

Conclusion
Overall, the findings of our investigation offer valuable understanding regarding the clinical relevance of ARGs and molecular subtypes in CRC, laying the groundwork for improved precision medicine applications and personalized treatment modalities.
Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells
Authors: Kozisek Tyler , Samuelson Luke , Hamann Andrew , Pannier Angela K. 
Published: December 2023

Abstract
Background
Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic potential. Furthermore, expression of multiple transgenes in hMSCs could greatly advance their clinical significance for treatment of multifaceted diseases, as individual transgenes could be expressed that target separate pathogenic drivers of complex diseases. Expressing multiple transgenes can be accomplished by delivering multiple DNA vectors encoding for each transgene, or by delivering a single poly-cistronic vector that encodes for each transgene and accomplishes expression through either use of multiple promoters, an internal ribosome entry site (IRES), or a 2A peptide sequence. These different transgene expression strategies have been used to express multiple transgenes in various mammalian cells, however, they have not been fully evaluated in difficult-to-transfect primary cells, like hMSCs. This study systematically compared four transgene expression and delivery strategies for expression of two reporter transgenes in four donors of hMSCs from two tissue sources using lipid- and polymer-mediate transfection, as follows: (i) delivery of separate DNA vectors in separate nanoparticles; (ii) delivery of separate DNA vectors combined in the same nanoparticle; (iii) delivery of a bi-cistronic DNA vector with an IRES sequence via nanoparticles; and (iv) delivery of a bi-cistronic DNA vector with a dual 2A peptide sequence via nanoparticles.

Results
Our results indicate that expression of two transgenes in hMSCs, independent of expression or delivery strategy, is inefficient compared to expressing a single transgene. However, delivery of separate DNA vectors complexed in the same nanoparticle, or delivery of a bi-cistronic DNA vector with a dual 2A peptide sequence, significantly increased the number of hMSCs expressing both transgenes compared to other conditions tested.

Conclusion
Separate DNA vectors delivered in the same nanoparticle and bi-cistronic DNA vectors with dual 2A peptide sequences are highly efficient at simultaneously expressing two transgenes in multiple donors of hMSCs from different tissue sources. The data presented in this work can guide the development of hMSC transfection systems for delivery of multiple transgenes, with the goal of producing clinically relevant, genetically modified hMSCs.
Eight-year chronic wound caused by Tarlov’s cyst: a case report
Authors: Huang Ya-Ning , Chang I-Wei , Li Sung-Tse , Lei Wei-Te 
Published: December 2023

Abstract
Background
Tarlov’s cyst is often underdiagnosed since it is difficult to identify without imaging assistance. Herein, we report the case of a young girl who presented with an 8-year history of chronic osteomyelitis of bilateral proximal phalanges and metatarsal bones caused by a Tarlov’s cyst that did not contain a nerve root. The chronic wound in the forefoot is an unusual presentation and resulted from the Tarlov’s cyst accompanied with tethered conus syndrome.

Case presentation
A 10-year-old Asian girl presented with an 8-year history of chronic osteomyelitis of bilateral proximal phalanges and metatarsal bones. She received sequestrectomy five times, however the immune function tests were all normal. A neurological examination revealed diminished sensation and a slapping gait pattern. Magnetic resonance imaging (MRI) demonstrated a lobulated cyst at the right aspect of the sacrum (S) 1 to sacrum (S) 3 canal near the dorsal root ganglion. Tethered conus syndrome was highly suspected. She received laminectomy of lumbar (L) 5 and S1–S2, which led to the diagnosis of a right S1–S3 epidural cyst. The final diagnosis from the histopathological examination was a right sacral Tarlov’s cyst. The clinical conditions of diminished sensation and slapping gait pattern greatly improved after successful surgical treatment.

Conclusion
In children who present with a recalcitrant chronic wound in the forefoot accompanied with a slapping gait pattern and foot hypoesthesia to pain, aggressive imaging examinations such as spine MRI should be arranged for further evaluation, especially in immunocompetent children.
Omission of Radiotherapy After Breast-Conserving Surgery for Women With Breast Cancer With Low Clinical and Genomic Risk: 5-Year Outcomes of IDEA
Authors: Jagsi Reshma , Griffith Kent A. , Harris Eleanor E. , Wright Jean L. , Recht Abram , Taghian Alphonse G. , Lee Lucille , Moran Meena S. , Small William , Johnstone Candice , Rahimi Asal , Freedman Gary , Muzaffar Mahvish , Haffty Bruce , Horst Kathleen , Powell Simon N. , Sharp Jody , Sabel Michael , Schott Anne , El-Tamer Mahmoud 
Published: December 2023

PURPOSE Multiple studies have shown a low risk of ipsilateral breast events (IBEs) or other recurrences for selected patients age 65-70 years or older with stage I breast cancers treated with breast-conserving surgery (BCS) and endocrine therapy (ET) without adjuvant radiotherapy. We sought to evaluate whether younger postmenopausal patients could also be successfully treated without radiation therapy, adding a genomic assay to classic selection factors. METHODS Postmenopausal patients age 50-69 years with pT1N0 unifocal invasive breast cancer with margins ≥2 mm after BCS whose tumors were estrogen receptor–positive, progesterone receptor–positive, and human epidermal growth factor receptor 2–negative with Oncotype DX 21-gene recurrence score ≤18 were prospectively enrolled in a single-arm trial of radiotherapy omission if they consented to take at least 5 years of ET. The primary end point was the rate of locoregional recurrence 5 years after BCS. RESULTS Between June 2015 and October 2018, 200 eligible patients were enrolled. Among the 186 patients with clinical follow-up of at least 56 months, overall and breast cancer–specific survival rates at 5 years were both 100%. The 5-year freedom from any recurrence was 99% (95% CI, 96 to 100). Crude rates of IBEs for the entire follow-up period for patients age 50-59 years and age 60-69 years were 3.3% (2/60) and 3.6% (5/140), respectively; crude rates of overall recurrence were 5.0% (3/60) and 3.6% (5/140), respectively. CONCLUSION This trial achieved a very low risk of recurrence using a genomic assay in combination with classic clinical and biologic features for treatment selection, including postmenopausal patients younger than 60 years. Long-term follow-up of this trial and others will help determine whether the option of avoiding initial radiotherapy can be offered to a broader group of women than current guidelines recommend.
The Iterates of the Frank–Wolfe Algorithm May Not Converge
Authors: Bolte Jérôme , Combettes Cyrille W. , Pauwels Edouard 
Published: December 2023

The Frank–Wolfe algorithm is a popular method for minimizing a smooth convex function f over a compact convex set [Formula: see text]. Whereas many convergence results have been derived in terms of function values, almost nothing is known about the convergence behavior of the sequence of iterates [Formula: see text]. Under the usual assumptions, we design several counterexamples to the convergence of [Formula: see text], where f is d-time continuously differentiable, [Formula: see text], and [Formula: see text]. Our counterexamples cover the cases of open-loop, closed-loop, and line-search step-size strategies and work for any choice of the linear minimization oracle, thus demonstrating the fundamental pathologies in the convergence behavior of [Formula: see text]. Funding: The authors acknowledge the support of the AI Interdisciplinary Institute ANITI funding through the French “Investments for the Future – PIA3” program under the Agence Nationale de la Recherche (ANR) agreement [Grant ANR-19-PI3A0004], the Air Force Office of Scientific Research, Air Force Material Command, U.S. Air Force [Grants FA866-22-1-7012 and ANR MaSDOL 19-CE23-0017-0], ANR Chess [Grant ANR-17-EURE-0010], ANR Regulia, and Centre Lagrange.
Prediction and simulation of a potential barrier block in Van der Waals heterojunction photodetectors
Authors: Liu Yangjun , Liang Haifeng , Li Yang 
Published: December 2023

The pBp structure can effectively suppress the dark current of a photodetector by blocking the majority of carriers. However, it is a big challenge to carry out large-scale simulation optimization for two-dimensional (2D) pBp heterojunction photodetectors due to a lack of the device models. Here, a numerical simulation model of the 2D pBp heterojunction was established based on the finite element method to solve this problem. Using this model, the spatial distribution of the energy band is clarified for each layer. The concentration of nonuniformly distributed electrons, induced by the incident light and bias voltage, is obtained by solving the diffusion and drift equations. The characteristics of the photocurrent and the dark current could be presented and the quantum efficiency could be calculated by counting the ratio of the number of carriers collected at the terminals and the carriers photogenerated. The material parameters could be modified for the optimization of the simulation and prediction. In using our model, a BP/MoS2/graphene photodetector was constructed, and the simulation results show that it works effectively under a reverse bias ranging from −0.3 to 0 V. The external quantum efficiency is 18%, while the internal efficiency approaches 85%. The doping in the barrier region definitely does not affect the dark current and the photocurrent. These results are similar to experimental results published earlier. In addition, with the BP bandgap width of 0.8 eV and incident wavelength of 1.7 µm, the dark current density predicted by the model could reach 3.3×10−8A/cm2, which is two orders lower than the reported 2D photodetectors at room temperature. This proposed model provides a way to design 2D pBp heterojunction photodetectors.
Does maternal health voucher scheme have association with distance inequality in maternal and newborn care utilization? Evidence from rural Bangladesh
Authors: Chowdhury Asiful Haidar , Hanifi Syed Manzoor Ahmed , Iqbal Mohammad , Hossain Aazia , Stones William , Amos Mark , Pallikadavath Saseendran , Bhuiya Abbas , Mahmood Shehrin Shaila 
Published: December 2023

Background
Bangladesh has made substantial progress in maternal health. However, persistent inequities in service use undermine the achievements at the national level. In 2007, the government introduced a Maternal Health Voucher Scheme (MHVS) to reduce barriers to service utilization. The current study explores the impact of MHVS on reducing distance inequality in utilization across the maternal and newborn continuum of care (MNCoC).

Methods
A cross-sectional survey was conducted from October’2017 to April’2018 in four selected MHVS sub-districts of Chattogram and Sylhet Divisions of Bangladesh. 2,400 women with at-least one child aged below two years were randomly selected. Both bivariate and multivariate analyses were carried out to explore the absolute and relative influence of the voucher scheme and chi-square test was used for hypothesis testing.

Results
Nineteen percent of the women were MHVS beneficiaries and 23% of them lived within 5 km of the health facility. Among the beneficiaries no significant differences were observed in the utilization of at-least 4 antenatal visits, skilled-assistance at delivery, postnatal care, and MNCoC between those living closer to the health facility and those living far away. However, a higher facility delivery rate was observed among beneficiary women living closer. By contrast, for non-beneficiaries, a significant difference was found in service use between women living closer to health facilities compared to those living further away.

Conclusion
The study found the use of MNCoC to be similar for all MHVS beneficiaries irrespective of their distance to health facilities whereas non-beneficiary women living further away had lower utilization rates. MHVS could have potentially reduced distance-related inequality for its beneficiaries. However, despite the provision of transport incentives under MHVS the reduction in inequality in facility delivery was limited. We propose a revision of the transportation incentive adjusting for distance, geographical remoteness, road condition, and transport cost to enhance the impact of MHVS.
The association between cardiopulmonary exercise testing and postoperative outcomes in patients with lung cancer undergoing lung resection surgery: A systematic review and meta-analysis
Authors: Arbee-Kalidas Nabeela , Moutlana Hlamatsi Jacob , Moodley Yoshan , Kebalepile Moses Mogakolodi , Motshabi Chakane Palesa 
Published: December 2023

Background
Exercise capacity should be determined in all patients undergoing lung resection for lung cancer surgery and cardiopulmonary exercise testing (CPET) remains the gold standard. The purpose of this study was to investigate associations between preoperative CPET and postoperative outcomes in patients undergoing lung resection surgery for lung cancer through a review of the existing literature.

Methods
A search was conducted on PubMed, Scopus, Cochrane Library and CINAHL from inception until December 2022. Studies investigating associations between preoperative CPET and postoperative outcomes were included. Risk of bias was assessed using the QUIPS tool. A random effect model meta-analysis was performed. I2 > 40% indicated a high level of heterogeneity.

Results
Thirty-seven studies were included with 6450 patients. Twenty-eight studies had low risk of bias. V˙O2 peak is the oxygen consumption at peak exercise and serves as a marker of cardiopulmonary fitness. Higher estimates of V˙O2 peak, measured and as a percentagege of predicted, showed significant associations with a lower risk of mortality [MD: 3.66, 95% CI: 0.88; 6.43 and MD: 16.49, 95% CI: 6.92; 26.07] and fewer complications [MD: 2.06, 95% CI: 1.12; 3.00 and MD: 9.82, 95% CI: 5.88; 13.76]. Using a previously defined cutoff value of > 15mL/kg/min for V˙O2 peak, showed evidence of decreased odds of mortality [OR: 0.55, 95% CI: 0.28–0.81] and but not decreased odds of postoperative morbidity [OR: 0.82, 95% CI: 0.64–1.00]. There was no relationship between V˙E/V˙CO2 slope, which depicts ventilatory efficiency, with mortality [MD: -9.60, 95% CI: -27.74; 8.54] however, patients without postoperative complications had a lower preoperative V˙E/V˙CO2 [MD: -2.36, 95% CI: -3.01; -1.71]. Exercise load and anaerobic threshold did not correlate with morbidity or mortality. There was significant heterogeneity between studies.

Conclusions
Estimates of cardiopulmonary fitness as evidenced by higher V˙O2 peak, measured and as a percentage of predicted, were associated with decreased morbidity and mortality. A cutoff value of V˙O2 peak > 15mL/kg/min was consistent with improved survival but not with fewer complications. Ventilatory efficiency was associated with decreased postoperative morbidity but not with improved survival. The heterogeneity in literature could be remedied with large scale, prospective, blinded, standardised research to improve preoperative risk stratification in patients with lung cancer scheduled for lung resection surgery.
CACTI: Co-developing awareness of bi/multilingual classroom practices with a professional development survey instrument
Authors: Mendoza Anna , Ou Jiaen , Rajendram Shakina , Coombs Andrew 
Published: December 2023

Abstract
This paper introduces an instrument, the Classroom Approaches to CLIL and Translanguaging Inventory (CACTI), to help primary and secondary teachers and academic researchers collaboratively develop awareness of bi/multilingual practices when academic subjects are taught in English. While teachers can develop explicit awareness of their language policies and practices, and of critical gaps that require action toward more equitable and productive learning spaces, researchers can learn what different bi/multilingual practices mean to teachers, and what concerns are salient for teachers regarding these practices. The practices may include (1) teaching for knowledge transfer across languages, (2) cultivating linguistic analysis skills, (3) raising critical awareness of the value placed on different languages in society, (4) developing bi/multilingualism and bi/multiliteracies, and (5) involving ALL students’ languages in the social and academic life of the class, even if they are not the official medium of instruction. Besides explaining what brought us to develop the CACTI, we illustrate two ways it can be used for collaborative reflection, one with pre-service teachers and one with in-service teachers. For each application, we discuss how our understanding was challenged by teachers, and what insights we offer for teachers on theirs.
Public History in Digital Spaces: Public Interpretations of the Transatlantic Slave Trade and Its Implications for History Teaching
Authors: Sosu Edmund S. , Boadu Gideon , Boateng Emmanuel B. , Appiah-Thompson Christopher 
Published: December 2023

Abstract
The Transatlantic Slave Trade was one of the cruelest events in human history, with its effects spanning several centuries. Slave monuments are visible representations of the memory of the slave trade and avenues for public discourse about the event and its impacts. This study draws on YouTube videos commemorating the Transatlantic Slave Trade in Ghana, examining not only the content of the videos but also the comments that YouTube users made on the videos. Based on the videos and comments, we analyze public sentiments, interpretations, and reconstruction of the Transatlantic Slave Trade. This study finds that social media presents opportunities to intensify public discourses about the Transatlantic Slave Trade. Public interpretations of the event convey a sense of optimism, forgiveness, reconciliation, and hope for a better and fairer world. Implications for the teaching of difficult histories in schools are discussed.
Traffic grooming with greedy-based priority routing and wavelength assignment for passive optical networks
Authors: Kumar Ashok , Bhat Shiveta , Aggarwal Sonam , Semwal Sunil , Batra Jyoti 
Published: December 2023

Abstract
Today, in passive optical networks (PON) the major issue is call blocking and it is getting worse as there is an increase in the number of connection requests but the wavelength channels in fiber links are limited. In this research, greedy-based priority routing and wavelength assignment traffic grooming (GPRWATG) technique is proposed aimed at reducing call blockage. In this scheme, to avoid optical–electrical–optical correspondence, firstly the grooming of connection requests with same source destination (s–d) is performed. According to the priority of these groomed connection requests, wavelength assignment and routing is assigned. This approach not only addresses the call blocking issue but also aligns with industry demands for improved network infrastructure. The proposed work performance is analyzed for blocking probability (BP), congestion, and its performance is compared with the non-priority-based routing and wavelength assignment traffic grooming (NPRWATG) and priority-based routing and wavelength assignment traffic grooming (PRWATG) schemes. The proposed method has 23.6 % lower congestion as compared to PRWATG and 21 % lower congestion as compared to NPRWATG. Also, the BP of GPRWATG is 26 % less than PRWATG and 21 % less than NPRWATG. Thus, it can be analyzed that by using the proposed technique, the BP as well as the congestion of the network altogether is reduced in comparison to the existing state-of-art techniques.