bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Function of the Adult Generation of Leydig Cells in Mice with Sertoli Cell-Selective or Total Ablation of the Androgen Receptor
Authors: De Gendt Karel , Atanassova Nina , Tan Karen A. L. , de França Luiz Renato , Parreira Gleydes Gambogi , McKinnell Chris , Sharpe Richard M. , Saunders Philippa T. K. , Mason J. Ian , Hartung Stefan , Ivell Richard , Denolet Evi , Verhoeven Guido 
Published: May 2005

AbstractIt is established that androgens and unidentified Sertoli cell (SC)-derived factors can influence the development of adult Leydig cells (LC) in rodents, but the mechanisms are unclear. We evaluated adult LC development and function in SC-selective androgen receptor (AR) knockout (SCARKO) and complete AR knockout (ARKO) mice. In controls, LC number increased 26-fold and LC size increased by approximately 2-fold between 12 and 140 d of age. LC number in SCARKOs was normal on d 12, but was reduced by more than 40% at later ages, although LC were larger and contained more lipid droplets and mitochondria than control LC by adulthood. ARKO LC number was reduced by up to 83% at all ages compared with controls, and LC size did not increase beyond d 12. Serum LH and testosterone levels and seminal vesicle weights were comparable in adult SCARKOs and controls, whereas LH levels were elevated 8-fold in ARKOs, although testosterone levels appeared normal. Immunohistochemistry and quantitative PCR for LC-specific markers indicated steroidogenic function per LC was probably increased in SCARKOs and reduced in ARKOs. In SCARKOs, insulin-like factor-3 and estrogen sulfotransferase (EST) mRNA expression were unchanged and increased 3-fold, respectively, compared with controls, whereas the expression of both was reduced more than 90% in ARKOs. Changes in EST expression, coupled with reduced platelet-derived growth factor-A expression, are potential causes of altered LC number and function in SCARKOs. These results show that loss of androgen action on SC has major consequences for LC development, and this could be mediated indirectly via platelet-derived growth factor-A and/or estrogens/EST.
Decreased Gonadotropin-Releasing Hormone Neuronal Activity Is Associated with Decreased Fertility and Dysregulation of Food Intake in the Female GPR-4 Transgenic Rat
Authors: Gomez Francisca , la Fleur Susanne E. , Weiner Richard I. , Dallman Mary F. , El Majdoubi Mohammed 
Published: May 2005

Abstract
Expression of a cAMP-specific phosphodiesterase in GnRH neurons in the GPR-4 transgenic rat resulted in decreased LH levels and pulse frequency and diminished fertility. We have characterized changes in fertility, adiposity, and reproductive and metabolic hormones with age. Although LH levels were decreased in 3-, 6-, and 9-month-old GPR-4 females relative to wild-type (WT) controls, GPR-4 females did not become anovulatory until 6 months of age. No differences were observed in FSH, estradiol, or androstenedione levels in 3-, 6-, or 9-month-old GPR-4 and WT females. At 9 months of age, GPR-4 females had significantly increased abdominal and sc fat depot weights that were associated with increased leptin and insulin levels not observed in WT females. We tested the hypothesis that metabolic changes observed at 9 months of age were the result of dysregulation of the mechanisms controlling energy balance. Two-month-old female GPR-4 rats placed on a high-energy diet gained weight at a rate significantly greater than WT females and, after 24 d, developed the same metabolic phenotype observed in 9-month-old GRP-4 females (increased abdominal and sc fat associated with elevated leptin and insulin concentrations). Overeating did not correlate with changes in estradiol or androstenedione levels. We conclude that decreased GnRH neuronal activity is closely associated with decreased reproductive function and dysregulation of food intake.
The Differential Effects of the Gonadotropin Receptors on Aromatase Expression in Primary Cultures of Immature Rat Granulosa Cells Are Highly Dependent on the Density of Receptors Expressed and the Activation of the Inositol Phosphate Cascade
Authors: Donadeu Francesc Xavier , Ascoli Mario 
Published: May 2005

AbstractSignaling pathways mediating the divergent effects of FSH and LH on aromatase in immature rat granulosa cells were studied by infecting cells with increasing amounts of adenoviral vectors for the human LH receptor (hLHR) or FSH receptor (hFSHR). Increasing amounts of Ad-hLHR, used at a multiplicity of infection (MOI) of 20 or 200 viable viral particles/cell, increased human chorionic gonadotropin (hCG) binding and hCG-induced cAMP and Akt phosphorylation, but inositol phosphates only increased in response to hCG in cells infected with 200 MOI Ad-hLHR. In contrast, hCG increased aromatase expression in cells infected with 20, but not in cells infected with 200, MOI Ad-hLHR. Cells infected with 20 or 200 MOI Ad-hFSHR showed increased hFSH binding and hFSH-induced Akt phosphorylation, but the hFSH-induced cAMP response was unchanged relative to control cells. However, hFSH was able to stimulate the inositol phosphate cascade in the Ad-hFSHR-infected cells, and the hFSH induction of aromatase was abolished. We also found that activation of C kinase or expression of a constitutively active form of Gαq inhibited the induction of aromatase by hFSH or 8Br-cAMP. We conclude that the differential effects of FSH and LH on aromatase in immature granulosa cells are highly dependent on gonadotropin receptor density and on the signaling pathways activated. We propose that aromatase is induced by common signals generated by activation of the FSHR and LHR (possibly cAMP and Akt) and that the activation of the inositol phosphate cascade in cells expressing a high density of LHR or FSHR antagonizes this induction.
Androgen Receptor Mutations Identified in Prostate Cancer and Androgen Insensitivity Syndrome Display Aberrant ART-27 Coactivator Function
Authors: Li Wenhui , Cavasotto Claudio N. , Cardozo Timothy , Ha Susan , Dang Thoa , Taneja Samir S. , Logan Susan K. , Garabedian Michael J. 
Published: May 2005

AbstractThe transcriptional activity of the androgen receptor (AR) is modulated by interactions with coregulatory molecules. It has been proposed that aberrant interactions between AR and its coregulators may contribute to diseases related to AR activity, such as prostate cancer and androgen insensitivity syndrome (AIS); however, evidence linking abnormal receptor-cofactor interactions to disease is scant. ART-27 is a recently identified AR N-terminal coactivator that is associated with AR-mediated growth inhibition. Here we analyze a number of naturally occurring AR mutations identified in prostate cancer and AIS for their ability to affect AR response to ART-27. Although the vast majority of AR mutations appeared capable of increased activation in response to ART-27, an AR mutation identified in prostate cancer (AR P340L) and AIS (AR E2K) show reduced transcriptional responses to ART-27, whereas their response to the p160 class of coactivators was not diminished. Relative to the wild-type receptor, less ART-27 protein associated with the AR E2K substitution, consistent with reduced transcriptional response. Surprisingly, more ART-27 associated with AR P340L, despite the fact that the mutation decreased transcriptional activation in response to ART-27. Our findings suggest that aberrant AR-coactivator association interferes with normal ART-27 coactivator function, resulting in suppression of AR activity, and may contribute to the pathogenesis of diseases related to alterations in AR activity, such as prostate cancer and AIS.
A group III steroid solution without antibiotic components: an effective cure for external otitis
Authors: Emgård Per , Hellström Sten 
Published: May 2005

The present study was undertaken to compare the clinical benefits of prescribing ear drops containing 0.05% solution of betamethasone dipropionate (BD), and ear drops containing hydrocortisone with oxytetracycline hydrochloride and polymyxin B (HCPB), for topical treatment of external otitis.Fifty-one patients were enrolled in this open randomized, parallel-group, multicentre study,performed in eight different ENT departments. The patients were randomly assigned to one of thetwo treatment groups: BD (n= 26) and HCPB (n= 25). Only ENT specialists investigated the patients. Bacterial and fungal cultures were raised on days 1 and 11, using swabbedmaterial from ear canals. Twice daily the patients recorded their symptoms during the acute phase, using special diary cards.BD proved a significantly more effective cure than HCPB during the acute
phase of external otitis and afforded a lower relapse frequency during a six-month follow-up period. The patients of the BD group were significantly less troubled by itching (p <0.01) than those in the HCPB group. On day 11, at the end of the acute phase, growth of bacteria (p= 0.03) and fungi (p < 0.01) was less frequent in the BD group than inthe HCPB group. No serious adverse events occurred, and those minor events observed were comparable between the two groups.Our conclusion is that the group III steroid solution, BD, cured the external otitismore effectively than did the HCPB solution, whether infected by bacteria or by fungi. No difference was evident regarding adverse effects. Furthermore, price favours a solution without any antibiotic component. In view of these observations, a group III steroid solution ought to be the preferred remedy for external otitis, whether infected or not.
Endoscopic sinus surgery for antrochoanal polyp using CO2 laser and/or microresector: a long–term result
Authors: Sato Kiminori , Nakashima Tadashi 
Published: May 2005

Endoscopic sinus surgery procedure using CO2 laser and/or microresector for paediatric and adult chronic sinusitis with antrochoanal polyp (ACP), with long-term follow-up, was examined. Twelve children and 13 adults underwent the surgical procedure.The following two techniques were used to remove the antral portion of an ACP with a 70°endoscope:(1) CO2 laser – The base of the ACP was vaporized and removed via an enlarged natural ostium with a pipe-guide handpiece with a deflective tip.(2) Microresector – The ACP was resected via
an enlarged natural ostium and/or nasoantral window opened under the inferior turbinate with the curved and straight blade of a microresector.In the endoscopic follow up for 10 to 57 months, no patient who underwent the primary surgery required reoperation for ACP recurrence. One out of
the seven patients who underwent secondary surgery required a revised operation with microresector and CO2 laser.
Referral letters: are we prioritizing consistently?
Authors: Pothier David D , Repanos Costa 
Published: May 2005

Objectives: To determine the levels of intra- and inter-grade variability of the vetting of general practice (GP) letters as well as the intra-rater reliability of letterprioritization.Design: Prospective assessment of letter vetting and questionnaire survey.Setting: Three otolaryngology secondary referral centres in Bristol and Bath, UK.Participants: Twelve consultants, nine registrars, four staff and associate specialists (SAS) and 16 senior house officers (SHOs) in otolaryngology.Methods: Fifty GP letters (not including ’fast-track’ referrals) addressed
to one of the ENT departments were chosen sequentially. These were anonymized, photocopied and included in the questionnaire to all participating staff. Participants were asked to vet the letters as ’urgent’, ’soon’ or ’routine’ according to supplied waiting time criteria. The same letters were sent out again six weeks later.Results: There was no significant difference between grades for the mean number of letters vetted into each category. Intra-grade variability was high; the number of letters vetted urgentvaried from one out of 50 to 15 out of 50 for the consultants.The intra-rater reliability was high.Conclusion: The grade of trainee seems to make little difference with regard to ability to prioritize referrals, but within grades there is little agreement on what constitutes an urgent referral. We suggest further research, looking at the final outcome of patients, needs to be done to try to establish evidence-based guidelines to assist with letter vetting.
Cerebellopontine angle arachnoid cysts in adult patients: what is the appropriate management?
Authors: Alaani A , Hogg R , Siddiq M A , Chavda S V , Irving R M 
Published: May 2005

Arachnoid cysts can occur at different intracranial sites, including the cerebellopontine angle (CPA). The incidence of arachnoid cysts is 1 per cent of all intracranial lesions. Recent advances in MRI (magnetic resonance imaging) scan techniques have led to CPA arachnoid cysts being more frequently diagnosed and with a higher degree of certainty. The need for further understanding of their natural history as well as for the development of a management rationale has been highlighted with this increased rate of diagnosis.We present a series of five adult patients with
different clinical presentations attributed to CPA arachnoid cysts. These lesions have a characteristic location in the posterior-inferior aspect of the CPA below the facial and vestibulocochlear nerves. These cysts did not show change in size on repeated MRI scan and the patients’ symptoms did not progress over the period of follow up. Our findings would support a conservative management approach to the majority of these cysts.
Famine, social disruption, and involuntary fetal loss: Evidence from chinese survey data
Authors: Cai Yong , Feng Wang 
Published: May 2005

AbstractRelying on half a million pregnancy histories collected from Chinese women in the late 1980s, we studied nearly a quarter century of self-reported miscarriages and stillbirths in China. Our results suggest that these two forms of involuntary fetal loss are affected not only by biological and demographic factors, such as the mother’s age, pregnancy order, and pregnancy history, but also by the mother’s social characteristics and the larger social environment. In this article, we focus on how two social and economic crises—the Great Leap Forward famine and the Cultural Revolution— resulted in elevated risks of miscarriage and stillbirth in the Chinese population.
Sex differences in morbidity and mortality
Authors: Case Anne , Paxson Christina 
Published: May 2005

Abstract
Women have worse self-rated health and more hospitalization episodes than men from early adolescence to late middle age, but are less likely to die at each age. We use 14 years ofdata from the u.s. National Health Interview Survey to examine this paradox. Our results indicate that the difference in self-assessed health between women and men can be entirely explained by differences in the distribution ofthe chronic conditions they face. This is not true, however, for hospital episodes and mortality. Men with several smoking-related conditions-including cardiovascular disease and certain lung disorders-are more likely to experience hospital episodes and to die than women who suffer from the same chronic conditions, implying that men may experience more-severe forms of these conditions. While some of the difference in mortality can be explained by differences in the distribution of chronic conditions, an equally large share can be attributed to the larger adverse effects of these conditions on male mortality. The greater effects ofsmoking-related conditions on men’s health may be due to their higher rates ofsmoking throughout their lives.
Three dimensions of the survival curve: horizontalization, verticalization, and longevity extension
Authors: Cheung Siu Lan Karen , Robine Jean-Marie , Tu Edward Jow-Ching , Caselli Graziella 
Published: May 2005

AbstractThree dimensions of the survival curve have been developed: (1) “horizontalization,” which corresponds to how long a cohort and how many survivors can live before aging-related deaths significantly decrease the proportion of survivors; (2) “verticalization,” which corresponds to how concentrated aging-related (“normal”) deaths are around the modal age at death (M); and (3) “longevity extension,” which corresponds to how far the highest normal life durations can exceed M. Our study shows that the degree of horizontalization increased relatively less than the degree of verticalization in Hong Kong from 1976 to 2001. After age normalization, the highest normal life durations moved closer to M, implying that the increase in human longevity is meeting some resistance.
By what measure? family time devoted to children in the united states
Authors: Folbre Nancy , Yoon Jayoung , Finnoff Kade , Fuligni Allison Sidle 
Published: May 2005

Abstract
We argue that previous research on time devoted to child care has paid insufficient attention to the conceptualization of care time. Three separate problems are evident. First, the conventional focus on explicit activities with children distracts attention from the larger responsibilities of “passive” care, which ranges from time when children are sleeping to time when they are in the same general area but are not engaged in an activity with parents. Second, the empirical analysis of activity time focuses almost exclusively on parents, overlooking the role of relatives such as grandmothers and siblings. Third, the measurement of active care time often ignores the impact of overlaps among both care providers and recipients. Our analysis of the Child Development Supplement of the Panel Study of Income Dynamics sheds light on these three problems and presents new measures of passive and active care time.
Market transition, educational disparities, and family strategies in rural china: New evidence on gender stratification and development
Authors: Hannum Emily 
Published: May 2005

Abstract
Two theoretical perspectives have dominated debates about the impact of development on gender stratification: modernization theory, which argues that gender inequalities decline with economic growth, and the “women in development” perspective, which argues that development may initially widen gender gaps. Analyzing cross-sectional surveys and time-series data from China, this article indicates the relevance of both perspectives: while girls’ educational opportunities were clearly more responsive than boys’ to better household economic circumstances, the era of market transition in the late 1970s and early 1980s failed to accelerate and, in fact, may have temporarily slowed progress toward gender equity.
Ethnic intermarriage in times of social change: The case of latvia
Authors: Monden Christiaan W. S. , Smits Jeroen 
Published: May 2005

Abstract
We gain insight into the dynamics of ethnic intermarriage in times of social change by studying marriages between Latvians and Russians (including Belarussians and Ukrainians) that occurred in Latvia before and after independence from the Soviet Union. Before independence, ethnic intermarriage was already rather common, involving about 17% of the marriages annually. Since independence, intermarriage between Russians and Latvians has increased substantially. Part of this increase can be explained by selective emigration, but at least half of it may be due to integrative processes. Although there were more marriages between Russian men and Latvian women before independence, the gender pattern reversed after independence. Intermarriage levels were the highest among the less educated, children of mixed couples, partners with similar educational levels, and people in the countryside.
Migration and relationship power among mexican women
Authors: Parrado Emilio A. , Flippen Chenoa A. , McQuiston Chris 
Published: May 2005

AbstractOur study drew on original data collected in Durham, NC, and four sending communities in Mexico to examine differences in women’s relationship power that are associated with migration and residence in the United States. We analyzed the personal, relationship, and social resources that condition the association between migration and women’s power and the usefulness of the Relationship Control Scale (RCS) for capturing these effects. We found support for perspectives that emphasize that migration may simultaneously mitigate and reinforce gender inequities. Relative to their nonmigrant peers, Mexican women in the United States average higher emotional consonance with their partners, but lower relationship control and sexual negotiation power. Methodologically, we found that the RCS is internally valid and useful for measuring the impact of resources on women’s power. However, the scale appears to combine diverse dimensions of relationship power that were differentially related to migration in our study.
Changes in children’s time with parents: A correction
Authors: Sandberg John F. , Hofferth Sandra L. 
Published: May 2005

Abstract
This article provides corrected estimates of the weekly time that 3- to 12-year-old children spent either directly engaged with their parents or with their parents accessible to them in 1997, replicating the figures presented in the original 2001 Demography article. The data come from the 1997 Child Development Supplement to the Panel Study of Income Dynamics. The results show a considerably greater increase in children’s total time in 1997 with mothers, fathers, or either parent than was shown in the original article. Some alternative estimates, likely reasons for the larger change, and the implications are discussed.
The age pattern of first-birth rates among U.S. women: The bimodal 1990s
Authors: Sullivan Rachel 
Published: May 2005

Abstract
Between 1990 and 2002, the age pattern of Type I first-birth rates (i.e., the hazard of a first birth) among U.S. women was bimodal. This pattern, driven by changing differential fertility patterns among racial and ethnic groups, reached its apex at the mid-1990s and had almost vanished by the decade’s end. Research on first-birth timing has tended to focus on Type II first-birth rates and therefore has failed to identify this larger, bimodal pattern. This article presents the benefits of using Type I rates, documents the emergence of the bimodal pattern via two new measures of bimodality, and uses a decomposition analysis to discuss the pattern’s causes.
Race, gender, and marriage: destination selection during the great migration
Authors: Curtis White Katherine J. , Crowder Kyle , Tolnay Stewart E. , Adelman Robert M. 
Published: May 2005

Abstract
Using historical census microdata, we present a unique analysis of racial and gender disparities in destination selection and an exploration ofhypotheses regarding tied migration in the historical context ofthe Great Migration. Black migrants were more likely to move to metropolitan areas and central cities throughout the period, while white migrants were more likely to locate in nonmetropolitan and farm destinations. Gender differences were largely dependent on marital status. Consistent with the "tied-migration" thesis, married women had destination outcomes that were similar to those of men, whereas single women had a greater propensity to reside in metropolitan locations where economic opportunities for women were more plentiful.
Inverse Problem in Diffuse Reflectance Spectroscopy: Accuracy of the Kubelka-Munk Equations
Authors: Loyalka S. K. , Riggs C. A. 
Published: May 2005

In diffuse reflectance spectroscopy the Kubelka-Munk equations have been used extensively. These equations provide simple solutions to the inverse problem of obtaining information on the scattering and absorption cross sections from reflected light. Proof is provided that the basic Kubelka-Munk equation [Formula: see text] should be replaced by the equation [Formula: see text] and that the Kubelka-Munk function [Formula: see text] should be replaced by the function [Formula: see text] Here r( x) is the reflectance; s is the scattering cross section (cm−1); a = ( k + s)/ s, where k is the absorption cross section (cm−1); and R∞ is the reflection coefficient of an infinitely thick sample. We note, however, that because of a redefinition of a carried out by Kubelka and Munk in the process of their calculations, the scattering cross section s calculated from their expression [Formula: see text] is correct. But the Kubelka-Munk theory still overestimates k by a factor of two.