bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in dietary habits during the first year at university
Authors: Edwards John S. A. , Meiselman Herbert L. 
Published: December 2004

SummaryLeaving home is a time when changes in dietary intake are likely. The purpose of this study was to measure changes in students’ dietary habits from leaving home in September to start their university course, to May the following year. Three cohorts (97, 123, 157) of first‐year students were surveyed over three consecutive years using food frequency questionnaires completed by students in September, late January and May of the following year (the same academic year). Reported intakes of energy, protein, fat and carbohydrate declined significantly (P < 0.05) from the period September–January for both the male and female groups and although there was a small decrease in energy intake for the male group for the period January–May and a small increase for the female group for the same period, none of these were significant. Reported intakes of alcohol in males increased in each of the three periods but none of these increases were significant. Reported intakes of most food items and the frequency with which they were consumed declined during the period September–January, but the reference nutrient intake was still met for many nutrients. Nonetheless, we consider it important to monitor food consumption, particularly when first leaving home, to ensure adequacy; and alcohol to ensure that total consumption does not take place in single binge sessions.
Positive margins in basal cell carcinoma: relationship to clinical features and recurrence risk. A retrospective study of 248 patients
Authors: Nagore E , Grau C , Molinero J , Fortea JM 
Published: December 2004

ABSTRACTBackground Incomplete resection of a basal cell carcinoma does not necessarily imply tumour recurrence.Objective The purpose of our study was to determine the clinical features most often associated with positive surgical margins and to establish whether positive margins effectively imply tumour recurrence.Methods We did a retrospective evaluation of 273 basal cell carcinomas in a total of 248 subjects. For each case, data regarding tumour location, sex, histological type and the presence or absence of affected surgical margins were collected. Follow‐up was available in 151 cases.Results Positive margins were most often observed in facial lesions, particularly in the nasal and perioral areas, and for morphoeic histological types. Tumours with margin involvement exhibited a higher recurrence rate (26%) than those with free margins (14%) over a 5‐year follow‐up period.Conclusions Individualized management, with special considerations depending on tumour location and histological type, is needed to treat basal cell carcinomas and cases with affected margins. Re‐excision, preferably with Mohs’ surgery, is advised in the latter as recurrences are much more complicated to treat. Furthermore, all cases need adequate follow‐up, even in cases with unaffected surgical margins.
Systemic 5‐fluorouracil producing an inflammatory response in porokeratosis
Authors: Nahm WK , Donohue KG , Danahy JF , Badiavas E , Falanga V 
Published: December 2004

ABSTRACTTopical 5‐fluorouracil has been used as an effective treatment for porokeratosis. Upon its treatment, an inflammatory effect occurs with the topical 5‐fluorouracil. We report a case of a patient with disseminated superficial actinic porokeratosis displaying a comparable inflammatory process following therapy with systemic 5‐fluorouracil used to manage a metastatic breast cancer.
Lichen planus and leukocytoclastic vasculitis induced by interferon alpha‐2b in a subject with HCV‐related chronic active hepatitis
Authors: De Sousa Pinto JM , Sacramento Marques M , Estanislau Correia T 
Published: December 2004

ABSTRACTLichen planus (LP) has been reported in association with chronic active hepatitis, primary biliary cirrhosis and other chronic liver diseases. The occurrence of LP in persons with hepatitis C virus (HCV) was reported by Robert et al., and the possible relationship between LP and hepatitis virus has also been supported by cases of LP following hepatitis B vaccination.Exacerbation or appearance of LP during the treatment of chronic hepatitis C, lymphoproliferative diseases and melanoma with alpha‐interferon (IFN‐α) and improvement of these diseases after discontinuation of this drug indicate that IFN‐α may possibly induce LP.We present a case of cutaneo‐mucous LP in a woman with chronic active hepatitis treated with IFN‐α and in whom local leukocytoclastic vasculitis was induced by the intradermal injection of a very low dose of IFN‐α.
Lichen planus and hepatitis C virus infection
Authors: Gimenez‐García R , Pérez‐Castrillón JL 
Published: December 2004

ABSTRACTBackground The association of lichen planus (LP) with liver diseases is well established. The reported prevalence rates of hepatitis C virus (HCV) antibodies in patients with LP tend to appear quite variable.Objective The aim of this study was to assess the prevalence of HCV antibodies in a group of patients with LP and evaluate the clinical characteristics of the subgroup with LP associated with HCV.Methods We studied 101 patients, 57 (56.4%) women and 44 (43.5%) men with a mean age of 48 years, consecutively diagnosed with cutaneous and/or mucosal LP between January 1992 and December 2000. We used 99 age‐ and sex‐matched controls.Results Anti‐HCV antibodies were detected in nine cases (8.9%) of the LP group but only two (2.02%) of the controls. The odds ratio between the subjects with HCV positivity and those with negative HCV virus was 4.74, with a confidence interval at 95%, between 0.999 and 22.545. A statistically significant association was only demonstrated between erosive LP and infection by HCV.Conclusions The possibility of liver disease caused by HCV should be ruled out in patients with LP, especially in the erosive form.
Cutaneous T‐cell lymphoma
Authors: Kotz EA , Anderson D , Thiers BH 
Published: December 2004

ABSTRACTCutaneous T‐cell lymphoma (CTCL) is a neoplasm of helper T cells whose first manifestations usually appear in the skin. The various forms of CTCL are distinguished by both clinical features and histopathology. Early on, the diagnosis may be difficult to establish because of its numerous, and often non‐specific, clinical presentations. Further, the pathological findings of early lesions may lack the diagnostic features observed in well‐developed or advanced disease. The diagnosis of CTCL must be considered in any patient with a chronic, therapy‐resistant condition of the skin. In patients with non‐specific histological findings, a high index of suspicion and multiple biopsies may eventually lead to a diagnosis of CTCL. Once the diagnosis of CTCL is established, accurate staging is essential both for its effect on treatment decisions and for its prognostic value. In general, CTCL is a chronic, slowly progressive disease with a long evolution. The development of tumours is a poor prognostic sign, as is erythroderma. The Sezary syndrome is a distinct form of erythrodermic CTCL that is characterized by exfoliative erythroderma, lymphadenopathy, lymphocytosis, intense pruritus, and circulating large, abnormal lymphocytes (Sezary cells). When death does occur, it is most often due to septicemia. Treatment of CTCL must be tailored to the individual patient. The most commonly employed treatment options are photochemotherapy and topical chemotherapy.
Specific immune therapy‐related cutaneous B‐cell pseudolymphoma with following dissemination
Authors: Gerlini G , Mori M , Massi D , Pimpinelli N 
Published: December 2004

ABSTRACTWe report two cases of cutaneous B‐cell pseudolymphoma (PCBCL) induced by intradermal antigen injections for specific immune therapy (SIT). In both cases, the lesions had first developed on the area of injection; years later, new lesions appeared far from the original site. The histological, immunohistochemical, and molecular findings of the lesions showed features consistent with the diagnosis of PCBCL in both cases. In particular, the staining for sIg light chains showed a polyclonal pattern, and the molecular analysis by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and Southern blot showed a germ‐line configuration of the Ig heavy chain genes. While the development of PCBCL related to a specific stimulus is well known and widely reported, the development of histologically and immunohistologically identical lesions far from the injection site is definitely worthy of note. This behaviour might be due to the presence of retained antigens in the injection site. This chronic antigenic stimulation could induce the progressive selection – and subsequent dissemination – of antigen‐specific B cell clones.
Neutrophilic eccrine hidradenitis heralding the onset of chronic myelogenous leukaemia
Authors: Gómez Vázquez M , Peteiro C , Toribio J 
Published: December 2004

ABSTRACTNeutrophilic eccrine hidradenitis was initially described in acute myelogenous leukaemic patients undergoing chemotherapy, suggesting a drug‐induced mechanism. It has been reported in some with various neoplastic and non‐neoplastic conditions as well as in healthy individuals. However, most the reported cases have been described in acute myelogenous leukaemic cases receiving chemotherapy. We describe a neutrophilic eccrine hidradenitis case unassociated with chemotherapy in a woman with chronic myelogenous leukaemia.
Drug‐induced sweat gland necrosis in a non‐comatose patient: a case presentation
Authors: Ferreli C , Sulica VI , Aste N , Atzori L , Pinna M , Biggio P 
Published: December 2004

ABSTRACTBackground Coma‐induced bullae and sweat gland necrosis is a rare clinicopathological entity often associated with drug‐induced coma.Subject We report a case with clinical and histopathologic findings characteristic of blisters and sweat gland necrosis occurring in a non‐comatose patient.Conclusions Skin blisters with underlying sweat gland necrosis is an entity previously reported to occur in comatose patients, our findings open new questions about the role of the drugs in the pathogenesis of those conditions.
Coexistence of pemphigus herpetiformis and systemic lupus erythematosus
Authors: Marinović B , Basta‐Juzbašić A , Bukvić‐Mokos Z , Ľeović R , Lončarić D 
Published: December 2004

ABSTRACTA female patient with coexistence of pemphigus herpetiformis and systemic lupus erythematosus is described. She presented to our Department with pruritic vesicles on her trunk and extremities, which were later accompanied with butterfly like erythema on her face and with central nervous system (CNS) manifestations. The diagnosis of pemphigus herpetiformis was based on the clinical picture and immunofluorescence finding, because the histopathologic finding is not always typical for the diagnosis. The diagnosis of systemic lupus erythematosus was based on positive ANA and anti‐dsDNA, presence of butterfly‐like erythema on her face, and CNS manifestations. The patient was treated by corticosteroids in combination with immunosuppressants, which should ensure good control of both diseases. The coexistence of pemphigus herpetiformis and systemic lupus erythematosus has not been reported in recent literature.
Psoriasis‐like dermoscopic pattern of clear cell acanthoma
Authors: Bugatti Leonardo , Filosa Giorgio , Broganelli Paolo , Tomasini Carlo 
Published: December 2004

ABSTRACTBackground Dermoscopy provides additional criteria for the diagnosis of skin lesions.Objective To investigate the dermoscopic aspect of clear cell acanthoma (CCA).Methods Six consecutive cases of CCAs were observed under conventional dermoscopy (Case 1–4) or videodermoscopy (Case 5–6).Results Common dermoscopic findings were: (i) squamous surface with translucid collarette; (ii) dilated capillary loops, mainly perpendicularly orientated to the skin surface, as dotted, pinpoint‐like structures, regularly distributed in a reticular array over the surface.Conclusions The vascular psoriasis‐like dermoscopic findings seem to be a clue for CCA and provide evidence of an inflammatory process for CCA formation.
Vitiligo in association with human immunodeficiency virus infection
Authors: Antony FC , Marsden RA 
Published: December 2004

ABSTRACTAutoimmune disorders such as vitiligo have been previously reported in patients with human immunodeficiency virus (HIV) infection. We describe a case of vitiligo in association with HIV where a rising CD4 lymphocyte count due to highly active antiretroviral therapy (HAART) closely correlated with changes in the skin. This effect, to our knowledge, has not been previously observed.
Oculocutaneous albinism
Authors: Okulicz JF , Shah RS , Schwartz RA , Janniger CK 
Published: December 2004

ABSTRACTOculocutaneous albinism represents a group of inherited skin disorders characterized by a generalized reduction of cutaneous, ocular and pilar pigmentation from the time of birth. Oculocutaneous albinism types 1 and 2 are the most common, but several other types have been described. A defect in the melanin synthesis pathway, resulting in reduced formation of melanin, is responsible for oculocutaneous albinism. Aetiology, clinical manifestations, diagnosis and management are discussed.
Is the combination of calcipotriol and PUVA effective in vitiligo?
Authors: Baysal V , Yildirim M , Erel A , Kesici D 
Published: December 2004

ABSTRACTObjective The objective was to compare the effectiveness of psoralen plus ultraviolet A (PUVA) and the combination of PUVA and topical calcipotriol in the treatment of vitiligo.Background There are several reports on the response rate of patients with vitiligo treated with the combination of PUVA and calcipotriol or calcipotriol alone.Subjects and methods Twenty‐two patients with generalized vitiligo were taken into the study. PUVA treatment was applied on a twice‐weekly schedule. Calcipotriol cream was applied to one of the two symmetrical lesions of each patient twice daily.Results Our results showed that the addition of topical calcipotriol to PUVA treatment did not lead to a significant increase in response rate of patients with vitiligo compared with PUVA treatment alone.
Aquagenic keratoderma
Authors: Betlloch I , Vergara G , Albares MP , Pascual J‐C , Silvestre J‐F , Botella R 
Published: December 2004

ABSTRACTAquagenic keratoderma has been described as a transitory condition involving young females and defined clinically by the appearance of palmar lesions accentuated after immersion in water. According to previous case reports, these lesions are characterized histologically by hyperkeratosis and dilated eccrine ducts.Some aberration in the eccrine ducts has been suggested as a possible pathogenic mechanism. We report a new case in a female adolescent. With regard to the normal aspect of the epidermis in our case, the clinical changes should be ascribed to a structural alteration of the horny layer without any visible microscopy change or functional alteration of the stratum corneum involving the organism in the adolescence period.
Twenty‐seven new microsatellites for the migratory Asian catfish family Pangasiidae
Authors: Hogan Zeb S. , May Bernie P. 
Published: December 2004

AbstractIn this paper, we describe primers for 27 new microsatellite loci tested on five species of migratory Asian catfish: Pangasius krempfi, P. bocourti, P. conchophilus, P. pleurotaenia, and Helicophagus waandersii. These primers were developed from a (GATA)n library created from the pooled DNA of three species of pangasiid catfish. All 27 loci are polymorphic in at least one species. Fifteen loci are polymorphic in at least three species. The primers described in this paper are thus shown to be useful in several species within the catfish family Pangasiidae, and may prove useful in additional species in future tests.
Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)
Authors: Endsley M. A. , Baker M. D. , Krafsur E. S. 
Published: December 2004

AbstractGenomic libraries from house flies enriched for (CA)15 and (CAG)10 repeats were constructed by using biotinylated probes. Twenty‐five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty‐seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 ± 2.5 and average heterozygosity was 72 ± 4%. FST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.
famoz: a software for parentage analysis using dominant, codominant and uniparentally inherited markers
Authors: Gerber S. , Chabrier P. , Kremer A. 
Published: December 2004

Abstract famoz (an acronym for father/mother) is a software useful in reconstructing parentage for dominant, codominant and uniparentally inherited markers. It is written in C and TclTk languages and is available for Unix, Linux and Windows systems at http://www.pierroton.inra.fr/genetics/labo/Software/Famoz/index.html. Parameters and assumptions used in the calculations are few and simple. Exclusion and identity probabilities, log‐likelihoods of any genetic relationship, potential father and parent or parent pair, half‐ and full‐sibship are calculated based on real or simulated data. Error rates for genotypic mistyping can be introduced. Simulations can be done to build statistical tests for parentage assignment.
Isolation and characterization of six microsatellite loci in the drywood termite Cryptotermes secundus (Kalotermitidae)
Authors: Fuchs A. , Heinze J. , Reber‐Funk C. , Korb J. 
Published: December 2004

AbstractThe Kalotermitidae is a family of lower termites and its species are ideal to study the evolution and maintenance of eusociality within the Isoptera. Co‐dominant genetic markers are an essential tool to elucidate the putatively complex colony and population structure. Six polymorphic microsatellite loci were identified in the drywood termite Cryptotermes secundus (Kalotermitidae). To test the variability of the markers, 18 colonies (n = 18) from Darwin (Australia) were assayed. We found two to eight alleles per locus with the level of observed heterozygosity at each locus ranging from 0.00 to 0.53. The significantly positive FIT value suggests that some kind of inbreeding does occur in this population.
Characterization of six polymorphic microsatellites for the polychaete tubeworm Hydroides elegans and cross‐species amplification in the congener Hydroides hexagonus
Authors: Pettengill J. B. , Hadfield M. G. , Schug M. D. , Wendt D. E. 
Published: December 2004

AbstractWe isolated and characterized six polymorphic microsatellite loci for the polychaete tubeworm, Hydroides elegans. Two additional loci were not reliably scorable and estimates of heterozygosity were obtained for the other six. In addition, cross‐species amplification was successful for two loci using the congener H. hexagonus. Given that few microsatellite loci are available for polychaetes, these markers will be useful in assessing dispersal and gene flow in H. elegans and probably also other polychaetes.
Microsatellite markers for the heavily exploited canary (Sebastes pinniger) and other rockfish species
Authors: Gomez‐Uchida D. , Hoffman E. A. , Ardren W. R. , Banks M. A. 
Published: December 2004

AbstractThe isolation and characterization of nine polymorphic microsatellite loci (eight tetranucleotide and one dinucleotide) from the canary rockfish Sebastes pinniger are described. Polymorphism at these loci revealed from six to 28 alleles, with expected heterozygosities ranging between 0.42 and 0.88, enabling high‐resolution genetic population structure investigation for this overfished species in the northeastern Pacific. They also amplify in 13 other congeneric species, providing highly variable loci for research on other rockfishes.
Characterization of microsatellite markers in the squid, Loligo bleekeri (Cephalopoda: Loliginidae)
Authors: Iwata Y. , Munehara H. , Sakurai Y. 
Published: December 2004

Abstract Loligo bleekeri has a long spawning season and the size of mature males changes during the season: dimorphic (large/small) early in the spawning season and monomorphic (small) later in the spawning season. To understand how copulatory behaviours relate to the dimorphism, we developed five polymorphic microsatellite loci in L. bleekeri. The level of polymorphism ranged from 10 to 22 alleles with expected heterozygosities ranging from 0.79 to 0.93, suggesting that the novel polymorphic loci should be useful for parentage analysis of L. bleekeri.
Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa
Authors: Alberto F. , Correia L. , Billot C. , Duarte C. M. , Serrão E. 
Published: December 2004

AbstractIn order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.
Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles maculipennis
Authors: Weill M. , Severini C. , Guillemin M.‐L. , Berticat C. , Berthomieu A. , Rousset F. , Raymond M. 
Published: December 2004

AbstractThe Anopheles maculipennis complex includes the most important malaria vectors of the western Palearctic. Anopheles maculipennis s.s., one member of this complex, is a reported vector in the Middle East. Here we describe the isolation of 15 microsatellite polymorphic loci from the An. maculipennis s.s. genome, displaying a high among individual diversity (0.37–0.77) in a sample from France. Three loci displayed a significant departure from Hardy–Weinberg proportions, suggesting a substantial frequency of null alleles. The remaining 12 loci are good candidates for further genetic studies in this species.
Identification of microsatellite markers in the red flour beetle, Tribolium castaneum
Authors: Pai Aditi , Sharakhov Igor V. , Braginets Olga , Costa Christina , Yan Guiyun 
Published: December 2004

AbstractWe isolated and characterized microsatellite markers for the red flour beetle Tribolium castaneum, an important model species for studies in various areas of evolutionary biology and ecology. A microsatellite‐enriched genomic library was constructed and screened with single stranded oligonucleotide probes [(CCT)17, (AAT)17 and (CAG)17]. Forty‐five primer pairs were designed of which 19 pairs produced successful amplification. Polymorphism screening involved beetles from five beetle strains and revealed 15 polymorphic and four monomorphic markers. The development of polymorphic microsatellite markers will facilitate future ecological and genetic studies involving T. castaneum beetles.
Development of microsatellite markers for the endangered freshwater pearl mussel Margaritifera margaritifera L. (Bivalvia: Unionoidea)
Authors: Geist Jürgen , Rottmann Oswald , Schröder Wolfgang , Kühn Ralph 
Published: December 2004

AbstractFreshwater pearl mussels (Margaritifera margaritifera L.) are among the most critically endangered freshwater invertebrates. We describe the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening 4900 recombinant clones from two genomic libraries. Thirteen loci revealed polymorphisms as demonstrated on 42 tested individuals from four river drainages. Allelic richness ranged from two to 12 alleles and averaged 6.8 alleles per locus with heterozygosity levels varying from 0 to 0.850 for observed heterozygosity (HO) and from 0.174 to 0.850 for expected heterozygosity (HE). Deficiency of heterozygous genotypes was observed in eight of 13 loci.