bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor antigens and immunogenic cell death subtypes guided mRNA vaccine development for lower-grade gliomas
Authors: Yin Wen , Xie Dongcheng , Tang Guihua , Ren Caiping , Jiang Xingjun 
Published: October 2023

Abstract
Background: Accumulating evidence demonstrated the effectiveness of mRNA vaccine against many cancers, however, their development in LGGs is still urgently needed. In addition, increasing evidence demonstrated that Immunogenic cell death (ICD) was associated with antitumor immune response. Thus, the aim of our study was to identify potential LGG tumor antigens for mRNA vaccine development and select suitable patients for vaccination based on ICD subtypes.
Methods: Gene expression matrix and matched clinical information of LGG were downloaded from the UCSC Xena website and CGGA databases. Differential expression analysis was conducted by GEPIA, and altered genomes were obtained from cBioPortal. TIMER was used for immune cell infiltration analysis, consensus clustering for typing ICD subtypes, and WGCNA for identifying hub modules and genes related to ICD subtypes. Eighty-two glioma tissue samples were collected and immunohistochemical staining was used to validate the correlation between tumor antigens and co-stimulatory factors.
Results: We identified seven potential LGG tumor antigens significantly correlated with poor prognosis and strongly positively correlated with infiltration of antigen-presenting cells, including CREB3L2, DDR2, IRF2, NCSTN, RECQL, REST, and TGFBR1. Furthermore, we identified two ICD subtypes in LGGs with different clinical, cellular, and molecular characteristics. Icds1 is an immunological "hot" and immunosuppression phenotype with a worse prognosis, while Icds2 is an immunological cold phenotype with a better prognosis. Finally, WGCNA identified hub immune-related genes associated with ICD subtypes, which could be potential vaccination biomarkers.
Conclusion: In summary, CREB3L2, DDR2, IRF2, NCSTN, RECQL, REST, and TGFBR1 are LGGs’ potential tumor antigens for mRNA vaccine development. The Icds2 subtype is suitable for vaccination.
Me and My AI Bot: Exploring the 'AIholic' Phenomenon and University Students' Dependency on Generative AI Chatbots - Is This the New Academic Addiction?
Authors: Salah Mohammed , Abdelfattah Fadi , Alhalbusi Hussam , Mukhaini Muna Al 
Published: October 2023

Abstract

Amidst the buzz of technological advancement in education, our study unveils a more disconcerting narrative surrounding student chatbot interactions. Our investigation has found that students, primarily driven by intrinsic motivations like competence and relatedness, increasingly lean on chatbots. This dependence is not just a preference but borders on an alarming reliance, magnified exponentially by their individual risk perceptions. While celebrating AI's rapid integration in education is tempting, our results raise urgent red flags. Many hypotheses were supported, pointing toward a potential over-dependence on chatbots. Nevertheless, the unpredictable outcomes were most revealing, exposing the unpredictable terrain of AI's role in education. It is no longer a matter of if but how deep the rabbit hole of dependency goes. As we stand on the cusp of an educational revolution, caution is urgently needed. Before we wholly embrace chatbots as primary educators, it is imperative to understand the repercussions of replacing human touch with AI interactions. This study serves as a stark wake-up call, urging stakeholders to reconsider the unchecked integration of chatbots in learning environments. The future of education may very well be digital, but at what cost to human connection and autonomy?
Sequence-related Amplified Polymorphism and Target Region Amplification Polymorphism Markers-based Profiling of Sodium Azide and Ethyl Methanesulfonate-derived Black Rot-resistant Dendrobium sp. ‘Earsakul’ Mutants from In Vitro Mutagenesis and Selection
Authors: Chueakhunthod Witsarut , Khairum Apinya , Jaukwon Piyangkoon , Chantakot Theerawat , Woramit Tanandorn , Hualsawat Sasitorn , Tantasawat Piyada A. 
Published: October 2023

To reveal the applicability of sequence-related amplified polymorphism (SRAP) and target region amplification polymorphism (TRAP) markers to mutant genotyping and marker identification for resistance to black rot (Phytophthora palmivora) in orchids (Dendrobium sp. ‘Earsakul’), we fingerprinted four nonmutagenized controls and 12 black rot-resistant mutants obtained with in vitro sodium azide (NaN3) and ethyl methanesulfonate (EMS) mutagenesis and in vitro selection using culture filtrate of P. palmivora. Each of the 20 SRAP and TRAP primer combinations yielded 375 and 384 scorable DNA bands, respectively, of which 94 (24.42%) and 88 (22.91%) were polymorphic, respectively. Mantel’s test cophenetic correlation coefficients of SRAP, TRAP, and SRAP/TRAP were 0.750, 0.921, and 0.861, respectively, indicating the efficiency of these markers, especially TRAP and SRAP/TRAP, for Dendrobium sp. ‘Earsakul’ mutant characterization. Moreover, the correlations between the matrices of cophenetic correlation values for the dendrograms of SRAP with TRAP, SRAP with SRAP/TRAP, and TRAP with SRAP/TRAP were 0.399, 0.566, and 0.793, respectively, and the dendrograms based on SRAP vs. TRAP and SRAP vs. SRAP/TRAP, with lower correlations, had more variations, i.e., the number of clusters, the members of clusters, and the placement of the materials, than the ones based on TRAP vs. SRAP/TRAP. Among the three dendrograms, all nonmutagenized controls were clustered together, whereas all the highly resistant and the most resistant mutants were distributed separately as individuals. Interestingly, the four SRAP and TRAP markers were significantly associated with black rot resistance. Overall, our results will be useful for facilitating future Dendrobium sp. ‘Earsakul’ breeding programs.
Optimization of phenolic compounds extraction from olive mill wastewater using response surface methodology
Authors: Shahkouhmahali Elnaz , Mohamadzadeh Jalal 
Published: October 2023

Abstract

Large amounts of pomace are produced during the extraction of olive oil. This is because only 21% of an olive's weight is made up of oil, while 79% includes water, bark and pulp. This project extracted total phenolic compounds (TPCs) from olive mill wastewater as efficiently as possible. The TPCs were analyzed and the total antioxidant activity (TAA) was evaluated by spectrophotometry. Data were evaluated using the response surface method (RSM). The largest TAA and TPC were discovered in extracting using 80% ethanol at 25 °C, and 2.5 bar with pH = 4. The highest amount of TPCs was 11.614 mg of gallic acid per 100 mL, and a value of 71.06% was reported for TAA. The results of the quadratic model showed that R2 is equal to 0.937, because it has a larger coefficient and the pH factor had the least effect. The temperature factor had the greatest impact on the extraction of TPC and TAA, and the mutual temperature and pH impacts affected the extraction positively. As a result, it can be concluded that the RSM was a useful tool for assessing the ideal circumstances for phenolic component extraction.
SGK-1 Signalling Pathway is a Key Factor in Cell Survival in Ischemic
Injury
Authors: Chaudhary Manisha , Sharma Veerta , Bedi Onkar , Kaur Amarjot , Singh Thakur Gurjeet 
Published: October 2023

Abstract:
Serum and glucocorticoid-regulated kinases (SGK) are serine/threonine kinases that belong
to AGC. The SGK-1, which responds to stress, controls a range of ion channels, cell growth,
transcription factors, membrane transporters, cellular enzymes, cell survival, proliferation and
death. Its expression is highly controlled by various factors such as hyperosmotic or isotonic oxidative
stress, cell shrinkage, radiation, high blood sugar, neuronal injury, DNA damage, mechanical
stress, thermal shock, excitement, dehydration and ischemia. The structural and functional deterioration
that arises after a period of ischemia when blood flow is restored is referred to as ischemia/
reperfusion injury (I/R). The current review discusses the structure, expression, function and
degradation of SGK-1 with special emphasis on the various ischemic injuries in different organs
such as renal, myocardial, cerebral, intestinal and lungs. Furthermore, this review highlights the
various therapeutic agents that activate the SGK-1 pathway and slow down the progression of I/R
injuries.
Discrepancies between Screening Sonography and Ultrasound in Emergency
Department – A Case Report
Authors: Miravent Sérgio , Figueiredo Teresa , Donchenko Inna , Duarte Lobo Manuel , Cruz Gualter , Pedro Almeida Rui 
Published: October 2023

Introduction::
This case report presents a discrepancy in sonographic findings between a screening sonography performed by a Sonographer in the Basic
Emergency Service (BES) and a subsequent ultrasound performed by a Radiologist physician in a Referral Hospital (RH). The aim of this report is
to discuss the possible reasons for the discrepancy and its implications for patient care.

Case Presentation::
A patient with a history of epigastric pain and vomiting underwent screening sonography in a BES, which suggested Intrahepatic Biliary Dilatation
Duct (IHBD) and main pancreatic duct dilatation. The patient was subsequently referred to the RH for further evaluation. However, the Radiologist
in the RH did not confirm any of the initial suspicions from BES through a normal ultrasound procedure. The discrepancy raises questions
regarding the quality of the screening ultrasound, misinterpretation of the BES images, or the potential for ambiguity in the point of care ultrasound
(POCUS) exam.

Conclusion::
The differences in sonographic findings between BES and RH, in this case, suggest that the improvement of the patient's clinical condition and
therapeutic interventions may have contributed to the discrepancy. Further investigation and standardization of POCUS training and interpretation
may improve diagnostic accuracy and patient outcomes.
Regulating miRNAs Expression by Resveratrol: Novel Insights based on
Molecular Mechanism and Strategies for Cancer Therapy
Authors: Keshavarzmotamed Atoosa , Mousavi Vahide , Masihipour Niloufar , Rahmati Atefe , Mousavi Dehmordi Rohollah , Ghezelbash Behrooz , Alimohammadi Mina , Mafi Alireza 
Published: October 2023

Abstract:
Resveratrol, a polyphenolic phytoalexin found in a wide range of plants, including grapes, berries, and peanuts, is an extensively researched
phytochemical with unique pharmacological capabilities and amazing potential to affect many targets in various cancers. Resveratrol's anti-cancer
activities are due to its targeting of a variety of cellular and molecular mechanisms and crucial processes involved in cancer pathogenesis, such as
the promotion of growth arrest, stimulation of apoptosis, suppression of cell proliferation, induction of autophagy, regulating oxidative stress and
inflammation, and improving the influence of some of the other chemotherapeutic agents. MicroRNAs (miRNAs) are non-coding RNAs that
modulate gene expression by degrading mRNA or inhibiting translation. MiRNAs serve critical roles in a wide range of biological activities, and
disruption of miRNA expression is strongly linked to cancer progression. Recent research has shown that resveratrol has anti-proliferative and/or
pro-apoptotic properties via modulating the miRNA network, which leads to the inhibition of tumor cell proliferation, the activation of apoptosis,
or the increase of traditional cancer therapy effectiveness. As a result, employing resveratrol to target miRNAs will be a unique and potential
anticancer approach. Here, we discuss the main advances in the modulation of miRNA expression by resveratrol, as well as the several miRNAs
that may be influenced by resveratrol in different types of cancer and the significance of this natural drug as a promising strategy in cancer
treatment.
Optimization and In Vitro Evaluation of Curcumin-loaded Calcium Alginate
Microbeads using Box-Behnken Design for Colorectal Cancer
Authors: Kumar Pandey Amit , Singh Sara Udaivir , Dwivedi Harinath 
Published: October 2023

Background and Objective::
After lung cancer and breast cancer, colorectal cancer (CRC) is the third most common type of cancer and has the second-highest fatality rate.
Curcumin is one such naturally occurring dietary compound that demonstrated promise to treat colon cancer. For that, the goal of the current study
was to coat curcumin with Eudragit S100 to treat colorectal cancer in the distal intestine release. The multiparticulate dosage form can increase the
solubility of curcumin in the colon environment, sustain the drug release, and protect the drug from abrupt degradation in the colon environment.
All of these alterations can enhance the colon tissue levels, especially in the colon cancer cells in the patients, and thereby can enhance the utility
of the therapy.

Methods::
By using the ionotropic gelation technique, the formulations were made. Moreover, Design Expert 13 used a three-factor, three-level Box-Behnken
design (BBD) in this work to optimize the formulation for colon-targeted drug delivery. The concentration of potassium alginate, the concentration
of calcium chloride, and the curing duration were considered independent variables, and prepared microbeads were refined to study their impacts
on entrapment effectiveness and particle size. Eudragit S100 was enteric coated on Calcium alginate beads with an improved core.

Results::
Regarding particle size and entrapment effectiveness, respectively, the polymer concentration and curing duration had a substantial impact. The
ideal concentrations of calcium chloride and potassium alginate were 15% w/v and 6% w/v, respectively, with a 20-minute curing time. With a
drug entrapment efficiency of 88.4%, the improved formulation had particles with a size of 708 μm. After 12 hours, 79.23±0.32% of the drug was
released following an enteric coating of Eudragit S100 of optimized calcium alginate microbeads (10% weight gain).

Conclusion::
The present study conclusively demonstrates the usefulness of a Box-Behnken design in the optimization of colon-targeted formulations. To
effectively treat colorectal cancer, enteric-coated calcium alginate microbeads can be administered orally to deliver curcumin precisely to the
colon.
An update on Tomato Flu Virus: A Mini-review
Authors: Ahmad Zargar Abrar , Kumar Arvind , Gangwar Ruby , Kumar Ranjeet , Sharma Amit 
Published: October 2023

Abstract:This article aims to highlight the most recent information on the “tomato flu” epidemic in India. A new disease has sprung out in several areas of India. The illness had a rash that was predominantly seen in children under the age of five and was highly infectious. It was known as “tomato flu” because the rash was excruciatingly painful, and the blisters were the size of tiny tomatoes. None of the afflicted children experienced problems that would have resulted in death. The focus of the therapy was primarily symptomatic, supportive care along with isolation and sustaining hygiene standards. The RNA virus Coxsackievirus A16, a member of the Picornaviridae family, was shown to be the cause of this disease. A detailed literature review was performed on the epidemiology, aetiology, evolution, transmission, outbreak, preventive measures and management and treatment of tomato flu. The leading prevention is preventing infected children and infants from sharing clothes, food, and toys. Few drugs are used to prevent fever, but no antiviral drugs or vaccines are available to prevent tomato fever. We came to the conclusion that a novel form of Coxsackievirus A16 may be responsible for the current pandemic of this disease in India.
Association Between Socioeconomic Status and Adherence to Fecal Occult Blood Tests in Colorectal Cancer Screening Programs: Systematic Review and Meta-Analysis of Observational Studies
Authors: Luo Zilin , Dong Xuesi , Wang Chenran , Cao Wei , Zheng Yadi , Wu Zheng , Xu Yongjie , Zhao Liang , Wang Fei , Li Jibin , Ren Jiansong , Shi Jufang , Chen Wanqing , Li Ni 
Published: October 2023

Background
Screening adherence is important in reducing colorectal cancer (CRC) incidence and mortality. Disparity in CRC screening adherence was observed in populations of different socioeconomic status (SES), but the direction and strength of the association remained unclear.

Objective
We aimed to systematically review all the observational studies that have analyzed the association between SES and adherence to organized CRC screening based on fecal occult blood tests.

Methods
We systematically reviewed the studies in PubMed, Embase, and Web of Science and reference lists of relevant reviews from the inception of the database up until June 7, 2023. Individual SES, neighborhood SES, and small-area SES were included, while any SES aggregated by geographic areas larger than neighbors were excluded. Studies assessing SES with any index or score combining indicators of income, education, deprivation, poverty, occupation, employment, marital status, cohabitation, and others were included. A random effect model meta-analysis was carried out for pooled odds ratios (ORs) and relative risks for adherence related to SES.

Results
Overall, 10 studies, with a total of 3,542,379 participants and an overall adherence rate of 64.9%, were included. Compared with low SES, high SES was associated with higher adherence (unadjusted OR 1.73, 95% CI 1.42-2.10; adjusted OR 1.53, 95% CI 1.28-1.82). In the subgroup of nonindividual-level SES, the adjusted association was significant (OR 1.57, 95% CI 1.26-1.95). However, the adjusted association was insignificant in the subgroup of individual-level SES (OR 1.46, 95% CI 0.98-2.17). As for subgroups of the year of print, not only was the unadjusted association significantly stronger in the subgroup of early studies (OR 1.97, 95% CI 1.59-2.44) than in the subgroup of late studies (OR 1.43, 95% CI 1.31-1.56), but also the adjusted one was significantly stronger in the early group (OR 1.86, 95% CI 1.43-2.42) than in the late group (OR 1.26, 95% CI 1.14-1.39), which was consistent and robust. Despite being statistically insignificant, the strength of the association seemed lower in studies that did not adjust for race and ethnicity (OR 1.31, 95% CI 1.21-1.43) than the overall estimate (OR 1.53, 95% CI 1.28-1.82).

Conclusions
The higher-SES population had higher adherence to fecal occult blood test–based organized CRC screening. Neighborhood SES, or small-area SES, was more competent than individual SES to be used to assess the association between SES and adherence. The disparity in adherence between the high SES and the low SES narrowed along with the development of interventions and the improvement of organized programs. Race and ethnicity were probably important confounding factors for the association.
Preferences in the Willingness to Download an mHealth App: Discrete Choice Experimental Study in Spain, Germany, and the Netherlands
Authors: Folkvord Frans , Bol Nadine , Stazi Giacomo , Peschke Lutz , Lupiáñez-Villanueva Francisco 
Published: October 2023

Background
Despite the worldwide growth in mobile health (mHealth) tools and the possible benefits for both patients and health care providers, the adoption of mHealth is low, and only a limited number of studies have examined the intention to download mHealth apps.

Objective
In this study, we investigated individuals’ preferences in the adoption of a health app.

Methods
We conducted a discrete choice experimental study in 3 countries (Spain: n=800, Germany: n=800, and the Netherlands: n=416) with 4 different attributes and levels (ie, price: €1.99 vs €4.99 [a currency exchange rate of €1=US $1.09 is applicable] vs for free, data protection: data protection vs no information, recommendation: patients’ association vs doctors, and manufacturer: medical association vs pharmaceutical company). Participants were randomly assigned. For the analyses, we used the conditional logistic model separately for each country.

Results
The results showed that price and data protection were considered important factors that significantly increased the probability to download an mHealth app. In general, the source of the recommendation and the manufacturer affected the probability to download the mHealth app less. However, in Germany and the Netherlands, we found that if the app was manufactured by a pharmaceutical company, the probability to download the mHealth app decreased.

Conclusions
mHealth tools are highly promising to reduce health care costs and increase the effectiveness of traditional health interventions and therapies. Improving data protection, reducing costs, and creating sound business models are the major driving forces to increase the adoption of mHealth apps in the future. It is thereby essential to create trustworthy standards for mobile apps, whereby prices, legislation concerning data protection, and health professionals can have a leading role to inform the potential consumers.
The relationships between variables of glycated hemoglobin and diabetes distress in patients with type 1 and type 2 diabetes mellitus
Authors: Pankiv V.I. , Yuzvenko T.Yu. 
Published: October 2023

Background. Diabetes distress is among potential barriers for people with diabetes mellitus (DM) to reach glycemic goals. Accumulating evidence suggests diabetes distress may be linked to individuals’ emotion regulation capacities. The purpose of this study was to explicate the relationships between variables of glyca­ted hemoglobin (HbA1c), diabetes distress, emotion regulation, and self-care variables through the analysis of cross-sectional data from individuals with type 1 and type 2 DM. Materials and methods. We used structural equation modeling to assess the cross-sectional relationships between variables of HbA1c, diabetes distress, emotion regulation, and self-care variables through the analysis of cross-sectional data from 132 individuals with type 1 and type 2 DM. After giving informed consent for their data to be used for research purposes without identifying them, study participants were examined by clinical psychologists with a structured clinical assessment and a series of other assessments relevant to DM. Results. While participants with type 2 DM differed from those with type 1 DM in terms of age, duration of DM, insulin dependence, and self-care, no significant diffe­rences were observed between the groups in sex, HbA1c levels, diabetes distress, emotion regulation-experience, or emotion regulation-skill scores. Study examined two potential explanatory models with one of them showing a more comprehensive view of the data revealing a total effect of poor emotional regulation on HbA1c levels. Diabetes distress in adults is linked to increased negative emotionality (emotion regulation-experience) and reduced skill at emotional regulation (emotion regulation-skill), both of which are associated with elevated HbA1c levels, and these relationships are stronger than those in diabetes self-care. Conclusions. This study suggests that in people with DM, elevated HbA1c levels and diabetes distress are linked to poor emotion regulation. These data emphasize that targeting difficulties in emotion regulation may hold promise for maximizing improvement in diabetes distress and HbA1c in individuals with DM.