bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary human myoblasts display only minor alternative polyadenylation compared to the transformed C2C12model of muscle differentiation
Authors: Varshney Akriti , Harrison Paul F. , Swaminathan Angavai , Alexander Sarah E. , Dichtl Bernhard , Lamon Séverine , Beilharz Traude H. 
Published: December 2023

AbstractAlternative polyadenylation has been linked to multiple developmental and disease transitions. The prevailing hypothesis being that differentiated cells use longer 3’ UTRs with expended regulatory capacity whereas undifferentiated cells use shorter 3’ UTRs. Here, we describe the gene expression and alternative polyadenylation profiles of human primary myoblasts over a time course of differentiation. Contrary to expectations, only minor changes in the 3’ end choice were observed. To reconcile this finding with published research, we devised a new bioinformatic method to compare the degree of alternative polyadenylation in the differentiation of primary human and immortalized murine (C2C12) myoblasts. Differentiated human primary myotubes display only half the alternative polyadenylation of the mouse model, with less than 1/10 of the genes undergoing alternative polyadenylation in C2C12cells showing evidence of alternative processing in human primary muscle differentiation. A global reduction in the expression of cleavage and polyadenylation factors in C2C12, but not in primary human myotubes may explain the lack of alternative polyadenylation in this system. Looking more broadly at transcriptome changes across differentiation shows that less than half of the genes differentially expressed in the immortalized model were recapitulated in primary cells. Of these, important metabolic pathways, such as glycolysis and sterol biosynthesis, showed divergent regulation. Collectively, our data caution against using immortalized cell lines, which may not fully recapitulate human muscle development, and suggest that alternative polyadenylation in the differentiation of primary cells might be less pronounced than previously thought.
Bayesian Hidden Mark Interaction Model for Detecting Spatially Variable Genes in Imaging-Based Spatially Resolved Transcriptomics Data
Authors: Yang Jie , Jiang Xi , Jin Kevin W. , Shin Sunyoung , Li Qiwei 
Published: December 2023

ABSTRACTRecent technology breakthroughs in spatially resolved transcriptomics (SRT) have enabled the comprehensive molecular characterization of cells whilst preserving their spatial and gene expression contexts. One of the fundamental questions in analyzing SRT data is the identification of spatially variable genes whose expressions display spatially correlated patterns. Existing approaches are built upon either the Gaussian process-based model, which relies onad hockernels, or the energy-based Ising model, which requires gene expression to be measured on a lattice grid. To overcome these potential limitations, we developed a generalized energybased framework to model gene expression measured from imaging-based SRT platforms, accommodating the irregular spatial distribution of measured cells. Our Bayesian model applies a zero-inflated negative binomial mixture model to dichotomize the raw count data, reducing noise. Additionally, we incorporate a geostatistical mark interaction model with a generalized energy function, where the interaction parameter is used to identify the spatial pattern. Auxiliary variable MCMC algorithms were employed to sample from the posterior distribution with an intractable normalizing constant. We demonstrated the strength of our method on both simulated and real data. Our simulation study showed that our method captured various spatial patterns with high accuracy; moreover, analysis of a seqFISH dataset and a STARmap dataset established that our proposed method is able to identify genes with novel and strong spatial patterns.
Ca2+Oscillation in Vascular Smooth Muscle Cells Control Myogenic Spontaneous Vasomotion and Counteract Post-ischemic No-reflow
Authors: Li Jinze , Zhang Yiyi , Zhang Dongdong , Wang Wentao , Xie Huiqi , Ruan Jiayu , Jin Yuxiao , Li Tingbo , Li Xuzhao , Zhao Bingrui , Zhang Xiaoxuan , Lin Jiayi , Shi Hongjun , Jia Jie-Min 
Published: December 2023

AbstractIschemic stroke produces the highest adult disability. Despite successful recanalization, no-reflow, or the futile restoration of the cerebral perfusion after ischemia, is a major cause of brain lesion expansion. However, the vascular mechanism underlying this hypoperfusion is largely unknown, and no approach is available to actively promote optimal reperfusion to treat no-reflow. Here, by combining two-photon laser scanning microscopy (2PLSM) and a mouse middle cerebral arteriolar occlusion (MCAO) model, we found myogenic vasomotion deficits correlated with post-ischemic cerebral circulation interruptions and no-reflow. Transient occlusion-induced transient loss of mitochondrial membrane potential (ΔΨm) permanently impaired mitochondria-endoplasmic reticulum (ER) contacts and abolished Ca2+oscillation in smooth muscle cells (SMCs), the driving force of myogenic spontaneous vasomotion. Furthermore, tethering mitochondria and ER by specific overexpression of ME-Linker in SMCs restored cytosolic Ca2+homeostasis, remotivated myogenic spontaneous vasomotion, achieved optimal reperfusion, and ameliorated neurological injury. Collectively, the maintaining of arteriolar myogenic vasomotion and mitochondria-ER contacts in SMCs, are of critical importance in preventing post-ischemic no-reflow.
Estimation of effective population size and effective number of breeders in an abundant and heavily exploited marine teleost
Authors: Bertram Andrea , Bell Justin , Brauer Chris , Fairclough David , Hamer Paul , Sandoval-Castillo Jonathan , Wellenreuther Maren , Beheregaray Luciano B. 
Published: December 2023

AbstractObtaining reliable estimates of the effective number of breedersN(b) and generational effective population size (Ne) for fishery-important species is challenging because they are often iteroparous and highly abundant, which can lead to bias and imprecision. However, recent advances in understanding of these parameters, as well as the development of bias correction methods, have improved the capacity to generate reliable estimates. We utilized samples of both single-cohort young of the year and mixed-age adults from two geographically and genetically isolated stocks of the Australasian snapper (Chrysophrys auratus) to investigate the feasibility of generating reliableNbandNeestimates for a fishery species. Snapper is an abundant, iteroparous broadcast spawning teleost that is heavily exploited by recreational and commercial fisheries. Employing neutral genome-wide SNPs and the linkage-disequilibrium method, we determined that the most reliableNbandNeestimates could be derived by genotyping at least 200 individuals from a single cohort. Although our estimates made from the mixed-age adult samples were generally lower and less precise than those based on a single cohort, they still proved useful for understanding relative differences in genetic effective size between stocks. The correction formulas applied to adjust for biases due to physical linkage of loci and age structure resulted in substantial upwards modifications of our estimates, demonstrating the importance of applying these bias corrections. Our findings provide important guidelines for estimatingNbandNefor iteroparous species with large populations. This work also highlights the utility of samples originally collected for stock structure and stock assessment work for investigating genetic effective size in fishery-important species.
Distinct host preconditioning regimens differentially impact the antitumor potency of adoptively transferred Th17 cells
Authors: Wittling Megen C. , Knochelmann Hannah M. , Wyatt Megan M. , Rangel Rivera Guillermo O. , Cole Anna C. , Lesinski Gregory B. , Paulos Chrystal M. 
Published: December 2023

AbstractBackgroundMechanisms by which distinct methods of host preconditioning impact the efficacy of adoptively transferred antitumor T helper cells is unknown.MethodsCD4+T cells with a transgenic TCR that recognize TRP-1 melanoma antigen were polarized to the T helper 17 (Th17) phenotype and then transferred into melanoma-bearing mice preconditioned with either total body irradiation or chemotherapy.ResultsWe found that preconditioning mice with a non-myeloablative dose of total body irradiation (TBI of 5 Gy) was more effective than using an equivalently dosed non-myeloablative chemotherapy (CTX at 200 mg/kg) at augmenting therapeutic activity of anti-tumor TRP-1 Th17 cells. Anti-tumor Th17 cells engrafted better following preconditioning with TBI and regressed large established melanoma in all animals. Conversely, only half of mice survived long-term when preconditioned with CTX and infused with anti-melanoma Th17 cells. IL-17 and IFN-g produced by the infused Th17 cells, were detected in animals given either TBI or CTX preconditioning. Interestingly, inflammatory cytokines (G-CSF, IL-6, MCP-1, IL-5, and KC) were significantly elevated in the serum of mice preconditioned with TBI versus CTX after Th17 therapy.ConclusionsOur results indicate, for the first time, that the antitumor response, persistence, and cytokine profiles resulting from Th17 therapy are impacted by the specific regimen of host preconditioning. This work is important for understanding mechanisms that promote long-lived responses by ACT, particularly as CD4+based T cell therapies are now emerging in the clinic.
The role of foreign board and ownership on the quality of sustainability disclosure: the moderating effect of social reputation
Authors: Zarefar Arumega , Agustia Dian , Soewarno Noorlailie 
Published: December 2023

Purpose
This study aims to examine the effect of social reputation on the relationship between boards and foreign ownership on the quality of sustainability disclosure.

Design/methodology/approach
The sample of this study consists of publicly-traded primary and secondary sector companies in Indonesia for 12 years, from 2009 to 2020. This study uses panel model regression to generate its results. The disclosure data are hand-collected data sourced from annual financial and company sustainability reports.

Findings
Higher foreign board component companies report lower quality of sustainability disclosure, whereas companies that possess foreign ownership components report a higher quality of sustainability disclosure. This result is strengthened by obtaining consistent results tested with economic, social and environmental disclosure components. In addition, if the company has a good social reputation, it will strengthen the relationship of foreign ownership to the quality of sustainability disclosure.

Practical implications
These findings are relevant for policymakers, professional organizations and practitioners in Indonesia and other developing countries.

Originality/value
The moderating effect of social reputation on the relation of the foreign board and foreign ownership-quality of sustainability disclosure as this study does remain rare in developing countries. This study complements various research conducted in developing countries, such as Indonesia, by offering a new dimension. The results indicate that social reputation has a moderating role in determining the impact of foreign ownership on the quality of sustainability disclosure.
Interview between Bruno Haghebaert and Ian Davis concerning the early days of disaster risk reduction 1970–2000
Authors: Davis Ian 
Published: December 2023

PurposeThe interview documents early days in the field of disaster risk reduction.Design/methodology/approachThe transcript and video were developed in the context of a United Nations Office for Disaster Risk Reduction (UNDRR) project on the History of DRR.FindingsThe transcript presents important developments during the 1980s with valuable lessons about risk reduction.Originality/valueIt takes the readers on a history of the journey of DRR over three decades.
Environmental protection versus economic growth in the views of Muslims: a large sample of empirical evidence
Authors: Sholihin Muhammad , Sugiyanto Catur , Susamto Akhmad Akbar 
Published: December 2023

Purpose
This research aims to examine the impact of religiosity and other control variables on Muslims’ environmental preservation and economic growth choices in 33 nations.

Design/methodology/approach
The study uses data from the World Values Survey (Waves 4–7) with a large sample size of 30,242 individuals. Logistic regression analysis is used to analyze the data, and the robustness principle is applied using the marginal effect of interaction variables method to select a viable model.

Findings
This study reveals that different aspects of religiosity – cognitive, affective and behavioral – positively impact the tendency of Muslims in 33 countries to prioritize environmental protection over economic progress. However, these influences vary significantly, as seen through odds ratios. In essence, the degree of religious devotion in these nations affects individuals’ leaning toward environmental preservation. This impact is further shaped by other factors such as politics, governance, economic development, environmental measures and legal frameworks.

Practical implications
The practical implication of this study is the development of an alternative theory that explains the conditions and categories under which religious beliefs and attitudes can influence the preferences of Muslims concerning environmental issues and economic growth.

Originality/value
This study fills a void in the body of literature by examining the nonlinear relationship between religiosity and individual Muslim preferences for environmental preservation and economic growth. It offers a framework for comprehending religion’s impact on Muslims’ redistributive individual preferences in these fields.
What enhances or worsens the user-generated metaverse experience? An application of BERTopic to Roblox user eWOM
Authors: Oh Yun Kyung , Yi Jisu , Kim Jongdae 
Published: December 2023

PurposeGiven its growing economic potential and social impact, this study aims to understand the motivations and concerns regarding metaverse usage. It identifies user needs and risks around the metaverse grounded on uses and gratifications theory and perceived risk theory.Design/methodology/approachThe authors analyzed user reviews and rating data from Roblox, a representative modern metaverse platform. They applied BERTopic modeling to extract topics from reviews, identifying key motivations and risk aspects related to metaverse usage. They further constructed an explanatory model to assess how those affect user satisfaction and changes in these effects over time.FindingsThis study discovered that gratifications like entertainment, escapism, social interaction and avatar-based self-expression significantly influence user satisfaction in the metaverse. It also highlighted that users find satisfaction in self-expression and self-actualization through creating virtual spaces, items and video content. However, factors such as identity theft, fraud and child safety were identified as potential detriments to satisfaction. These influences fluctuated over time, indicating the dynamic nature of user needs and risk perceptions.Research limitations/implicationsThe novelty of this study lies in its dual application of the uses and gratifications theory and perceived risk theory to the metaverse. It provides a novel perspective on user motivations and concerns, shedding light on the distinct elements driving user satisfaction within the metaverse. This study unravels the metaverse’s unique capacity to assimilate features from established digital media while offering a distinctive user-generated experience. This research offers valuable insights for academics and practitioners in digital media and marketing.Originality/valueThis research pioneers the application of both uses and gratifications and perceived risk theories to understand factors influencing metaverse satisfaction. By establishing a comprehensive framework, it explores the metaverse’s unique value as a user-content creation platform, while encompassing existing digital platform characteristics. This study enriches the academic literature on the metaverse and offers invaluable insights for both metaverse platforms and brand marketers.
Impact of agricultural credit sources heterogeneity on rice production efficiency in Côte d'Ivoire
Authors: Ouattara N'Banan , Xiong Xueping , Ali Abdelrahman , Sedebo Dessalegn Anshiso , Youan Bi Trazié Bertrand Athanase , Ballo Zié 
Published: December 2023

PurposeThis study examines the impact of agricultural credit on rice farmers' technical efficiency (TE) in Côte d'Ivoire by considering the heterogeneity among credit sources.Design/methodology/approachA multistage sampling technique was used to collect data from 588 randomly sampled rice farmers in seven rice areas of the country. The authors use the endogenous stochastic frontier production (ESFP) model to account for the endogeneity of access to agricultural credit.FindingsOn the one hand, agricultural credit has a significant and positive impact on rice farmers' TE. Rice farmers receiving agricultural credit have an average of 5% increase in their TE, confirming the positive impact of agricultural credit on TE. On the other hand, the study provides evidence that the impact of credit on rice production efficiency differs depending on the source of credit. Borrowing from agricultural cooperatives and paddy rice buyers/processors positively and significantly influences the TE, while borrowing from microfinance institutions (MFIs) negatively and significantly influences the TE. Moreover, borrowing from relatives/friends does not significantly influence TE.Research limitations/implicationsFuture research can further explore the contribution of agricultural credit by including several agricultural productions and using panel data.Originality/valueThe study provides evidence that the impact of agricultural credit on agricultural production efficiency depends on the source of credit. This study contributes to the literature on the impact of agricultural credit and enlightens policymakers in the design of agricultural credit models in developing countries, particularly Côte d'Ivoire.
How do family support factors influence college students’ online-startup thinking?
Authors: Li Lifu , Kang Kyeong 
Published: December 2023

Purpose
The purpose of this study is to present the relationship between family support factors and Chinese college students’ online-startup thinking on live streaming platforms. Considering China's specific online entrepreneurial environment, this paper divides Chinese college students’ online-startup thinking according to the liberal–conservative thinking theory. This study classifies family support factors based on the tangible–intangible resource division theory. Different tangible and intangible factors have different impacts on their online-startup thinking.

Design/methodology/approach
This study tests 588 samples based on the partial least squares path modelling and variance-based structural equation modelling. This study promotes importance-performance map analysis to explore additional findings of influencing factors and provide suitable suggestions for Chinese college students and related departments.

Findings
Tangible family support factors, such as labour resources support, and intangible family support factors, such as verbal encouragement, can positively enhance Chinese college students’ liberal thinking to online-startup and decrease their conservative thinking. Meanwhile, according to importance-performance map analysis results, verbal encouragement from the intangible unit instead of financial resource support from the tangible unit has a higher total effect and performance on Chinese college students’ liberal thinking and conservative thinking.

Originality/value
This study draws on psychology research based on Chinese college students’ unique entrepreneurial mentality. This paper divides Chinese college students’ thinking in online-startups into liberal thinking and conservative thinking based on the liberal–conservative thinking theory. Meanwhile, according to the feature of Chinese family support factors, this paper classifies various elements based on the tangible–intangible resource division theory, which is helpful for scholars to understand that the student perceptions of the value of family support are critical to the success of the online-startup.
The effects of virtual reality travel on satisfaction and visiting intention utilizing an extended stimulus-organism-response theory: perspectives from Thai tourists
Authors: Sann Raksmey , Luecha Pakkapol , Rueangchaithanakun Rawisara 
Published: December 2023

PurposeThis study investigates how virtual reality (VR) travel attributes (e.g. sense and quality of information) influence spectators' flow experience, how emotion and past experience affect enjoyment and examines the impact of flow experience and enjoyment on satisfaction and booking or visiting intention.Design/methodology/approachThe VR tour stimuli were fabricated using scenic views from the National Aquarium in the USA. Participants were equipped with Matterport VR and audio headsets and started their virtual travel. Once the participants completed their VR tours, they were asked to complete the questionnaire. Using the stimulus-organism-response theory, 303 valid responses were analyzed using partial least square structural equation modeling (PLS-SEM).FindingsThe results showed that the sense and quality of information in VR travel positively and significantly impacted the flow experience. Moreover, emotions and past experiences positively and significantly influenced the enjoyment of VR travel. Similarly, flow experience and enjoyment positively and significantly affect satisfaction. However, satisfaction with VR-related tourism experiences negatively affects users' bookings and visiting intentions.Practical implicationsThis study concludes that, from Thai tourists' perspectives, virtual travel should be used as a solution only during the pandemic because, in the long term it can cause a loss to the business chain in the tourism industry.Originality/valueTo the best of the authors' knowledge, no prior research has examined the influence of past experiences and emotions on satisfaction with VR travel.