bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of serum docosahexaenoic acid and eicosapentaenoic acid levels with dietary intakes and supplement use during pregnancy: a prospective observational study
Authors: Wakabayashi Nana , Haruna Megumi , Yonezawa Kaori , Sasagawa Emi , Usui Yuriko , Ohori Riko , Aoyama Satoko , Sasaki Satoshi , Nagamatsu Takeshi ,   Tokyo area members of the J-PEACH Study group as of 2019–2022
Published: December 2023

Abstract
This study aimed to determine the association of serum docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) levels with dietary intakes and supplement use during pregnancy. This prospective observational study was conducted at a university hospital in Tokyo, Japan. Participants in their second and third trimesters were given a self-administered questionnaire assessing the frequency of DHA and EPA supplement use in the past month and a brief-type self-administered diet history questionnaire. Non-fasting serum DHA and EPA levels were analysed using gas chromatography. Differences in biomarkers by frequency of supplement use were determined using multiple comparison analyses, and Spearman's correlation coefficient was used to determine biomarkers and DHA and EPA intakes by food group. Of the 116 participants, 11 (9⋅5 %) in the second trimester and 18 (15⋅5 %) in the third trimester regularly used supplements (≥5 times per week). Regular users had higher serum DHA and EPA levels than never users in the second and third trimesters. Dietary DHA and EPA intake from fish and shellfish was positively correlated with serum DHA and EPA in the second and third trimesters. Supplement use ≥5 times per week and fish and shellfish intake were associated with high serum DHA and EPA levels.
Feed energy utilization by hair sheep: does the 0.82 conversion factor of digestible to metabolizable energy need to be revised?
Authors: Brito Neto A. S. , Herbster C. J. L. , Geraseev L. C. , Macedo Junior G. L. , Nascimento D. R. , Rocha A. C. , Pereira M. I. B. , Marcondes M. I. , Silva L. P. , Bezerra L. R. , Oliveira R. L. , Pereira E. S. 
Published: December 2023

AbstractThe objective was to evaluate energy partitioning and predict the relationship between metabolizable energy (ME) and digestible energy (DE) in hair sheep fed tropical diets at three feeding levels (maintenance, intermediate and high). To evaluate the energy partition, a database with 114 records (54 non-castrated males and 60 females) from comparative slaughter studies was used. To estimate the ratio ME:DE, 207 observations (74 non-castrated males and 133 females) were used from six studies in a multi-study approach, two indirect calorimetry studies (n= 93) and four comparative slaughter (n= 114), using a mixed model and study as random effect. A simple linear regression equation of the ME against DE was fitted to predict the efficiency of DE to ME conversion. Gas losses were greatest (P< 0.05) for animals fed at maintenance level (7.92% of gross energy intake). The variations of energy losses in the urine were 2.64, 2.06 and 2.08%; faecal losses were 34.37, 37.80 and 36.91% for maintenance, intermediary and high level of feeding, respectively. The regression analysis suggested a strong linear relationship between ME and DE, generating the model ME (MJ/day) = −0.1559 (±0.07525) + 0.8503 (±0.005864) × DE (MJ/day). This study highlights the importance of the relationship ME:DE. Equation/factor 0.85 presented herein is alternative that could be used for the calculation of ME from DE in feedlot diets tropical. In conclusion, we suggest that for hair sheep fed tropical diets the conversion factor 0.85 is more adequate to predict ME from DE.
Navigating the research–practice relationship: Professional goals and constraints
Authors: Sato Masatoshi 
Published: December 2023

AbstractIn this article, which is based on my AAAL 2023 plenary talk, I argue that researchers may be contributing to widening the never-ending gap between research and practice. At least, there is such a possibility given that researchers, including myself, have rarely investigated their own beliefs and practices related to classroom teaching. In the first part, I overview research of the research–practice relationship and problematize the epistemological clash between two groups of professionals, that is, teachers and researchers. In the second part, I focus on researchers as a profession who have significant potential in contributing to education. In the third part, I share concrete ways of tackling researchers' obstacles in communicating with practitioners if, and when, they wish to be useful for real-world education, including: (a) adjusting the nature of research, and (b) improving communication methods. In conclusion, I propose a model in which the research–practice relationship can be more equitable, effective, and mutually beneficial.
Transforming African musics at a South African university
Authors: Bruinders Sylvia 
Published: December 2023

AbstractThis article critically engages with the discipline of African musics in the academy. It examines the process of curriculum transformation of the African music section at the South African College of Music, University of Cape Town since 2005 as an emergent curriculum model for an integrated approach to the teaching of African musics at universities. The adoption of this curriculum predated the 2015–2016 fervent calls in South Africa to decolonise the university, which necessitates an approach to teaching African musics not rooted in its colonial past. As is known, the study and research of African musics in the academy partly stemmed from the efforts of European (and later US) researchers who often received support through the British colonial administration and much of their output seemed to be focused on convincing their peers about the virtues of ‘African music’ and the study thereof. Despite this history, there is evidence to support the fact that in most African universities, the music departments are far more interested in teaching Western art music and its virtues. In recognition of the calls by students for radical changes to the curricula in South African universities, the article seeks to answer the question ‘how do we consider ongoing changes to the knowledge both received and produced in this field?’
“A Mission Without Precedent”: The Rise and Fall of the Office of Economic Opportunity, 1964–1981
Authors: LAROCHELLE RYAN 
Published: December 2023

AbstractThis article traces the history of the Office of Economic Opportunity/Community Services Administration, focusing on Richard Nixon’s failed attempt to dismantle it in 1973 and Ronald Reagan’s successful effort in 1981. I explore main two main questions: Why was Reagan able to succeed when Nixon had failed? and What does the dismantling of the agency reveal about the development of American conservatism in the 1970s and 1980s? Drawing on original archival materials, I argue that the Reagan administration learned from Nixon’s failures and adopted a more professional, managerial stance when it dismantled the agency in 1981. In addition, recent work in history and political science has explored how the multiracial democratic vision articulated by LBJ’s Great Society helped fuel the modern conservative movement. By focusing on the long-term opposition against OEO/CSA, this article provides new insights into how conservatives articulated an alternative ideology to postwar liberalism.
Satellite Video Remote Sensing for Estimation of River Discharge
Authors: Masafu Christopher , Williams Richard , Hurst Martin D. 
Published: December 2023

AbstractWe demonstrate that river discharge can be estimated by deriving water surface velocity estimates from satellite‐derived video imagery when combined with high‐resolution topography of channel geometry. Large Scale Particle Image Velocimetry (LSPIV) was used to map surface velocity from 28 s of 5 Hz satellite video acquired at a 1.2 m nominal ground spacing over the Darling River, Tilpa, Australia, during a 1‐in‐5‐year flood. We stabilized and assessed the uncertainty of the residual motion induced by the satellite platform, enhancing our sub‐pixel motion analysis, and quantified the sensitivity of image extraction rates on computed velocities. In the absence of in situ observations, LSPIV velocity estimates were validated against predictions from a calibrated 2D hydrodynamic model. Despite the confounding influence of selecting a surface velocity depth‐averaging coefficient, inference of discharge was within 0.3%–15% compared with gauging station measurements. These results provide a valuable foundation for refining satellite video LSPIV techniques.
Multi‐Instrument Observations of Various Ionospheric Disturbances Caused by the 6 February 2023 Turkey Earthquake
Authors: Haralambous Haris , Guerra Marco , Chum Jaroslav , Verhulst Tobias G. W. , Barta Veronika , Altadill David , Cesaroni Claudio , Galkin Ivan , Márta Kiszely , Mielich Jens , Kouba Daniel , Buresova Dalia , Segarra Antoni , Spogli Luca , Rusz Jan , Zedník Jan 
Published: December 2023

AbstractIn this work, we investigate various types of ionospheric disturbances observed over Europe following the earthquake that occurred in Turkey on 6 February 2023. By combining observations from Doppler sounding systems, ionosondes, and GNSS receivers, we are able to discern different types of disturbances, propagating with different velocities and through different mechanisms. We can detect co‐seismic ionospheric disturbances close to the epicenter, as well as ionospheric signatures of acoustic waves propagating as a consequence of propagating seismic waves. Unlike the vast majority of past ionospheric co‐seismic disturbance studies that are primarily based on Total Electron Content variations, reflecting disturbances propagating around the F‐region peak, the focus of the present study is the manifestation of disturbances at different ionospheric altitudes by exploiting complementary ionospheric remote sensing techniques. This is particularly highlighted through ionospheric earthquake‐related signatures established as specific ionogram deformations known as multiple‐cusp signatures which appear as additional cusps at the base of the F‐region attributed to electron density irregularities generated by Rayleigh surface waves that generate acoustic waves propagating up to the ionosphere. Therefore this study underlines the advantage that multi‐instrument investigations offer in identifying the propagation of earthquake‐related ionospheric disturbances at different ionospheric altitudes and distances from the earthquake epicenter.
Quantifying Photodegradation of Peatland‐Derived Dissolved Organic Carbon in the Coastal Ocean of Southeast Asia
Authors: Zhou Yongli , Müller Moritz , Cherukuru Nagur , Martin Patrick 
Published: December 2023

AbstractSoutheast Asia's peatlands are a globally significant source of terrigenous dissolved organic carbon (tDOC) to the ocean, and field observations show that this tDOC is extensively remineralized within the shelf sea. Yet the processes that drive this remineralization remain unclear. Here, we combined incubation experiments and model simulations to quantify the rate and extent of photodegradation of tDOC in the Sunda Shelf Sea. During laboratory photodegradation experiments, 26%–74% of the peatland tDOC was photomineralized, but realistic in situ rates of photodegradation have not yet been estimated in this region. Based on spectrally resolved apparent quantum yields for tDOC remineralization calculated from experiments, modeled in situ solar irradiance, and measured in‐water inherent optical properties, we simulated peatland tDOC photomineralization for two coastal regions of the Sunda Shelf Sea. These simulations show that sunlight can directly remineralize 25 ± 9% of the tDOC input over its maximum 2‐year residence time in the Sunda Shelf Sea, accounting for 38% of the total tDOC remineralization. We also found that photobleaching can remove 54 ± 4% of colored dissolved organic matter over this time‐scale. We further derived a simplified photochemical decay constant ∅ref of 0.016 day−1 for Southeast Asia's peatland‐derived tDOC, which can be used to parameterize the recently proposed UniDOM model. We conclude that direct photodegradation may be a greater sink for tDOC in Southeast Asia's coastal ocean compared to higher latitudes, although it is insufficient to account for the total tDOC remineralization observed in the Sunda Shelf Sea.
Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma
Authors: Zhang Xiaoping , Zhong Yong , Liu Lin , Jia Chengyou , Cai Haidong , Yang Jianshe , Wu Bo , Lv Zhongwei 
Published: December 2023

AbstractRecurring evidence suggests that fasting has extensive antitumor effects in various cancers, including papillary thyroid carcinoma (PTC). However, the underlying mechanism of this relationship with PTC is unknown. In this study, we study the effect of fasting on glycolysis and mitochondrial function in PTC. We find that fasting impairs glycolysis and reduces mitochondrial dysfunction in vitro and in vivo and also fasting in vitro and fasting mimicking diets (FMD) in vivo significantly increase the expression of lncRNA-protein kinase C theta antisense RNA 1 (PRKCQ-AS1), during the inhibition of TPC cell glycolysis and mitochondrial function. Moreover, lncRNA PRKCQ-AS1 was significantly lower in PTC tissues and cells. In addition, PRKCQ-AS1 overexpression increased PTC cell glycolysis and mitochondrial function; PRKCQ-AS1 knockdown has the opposite effect. On further mechanistic analysis, we identified that PRKCQ-AS1 physically interacts with IGF2BPs and enhances protein arginine methyltransferases 7 (PRMT7) mRNA, which is the key player in regulating glycolysis and mitochondrial function in PTC. Hence, PRKCQ-AS1 inhibits tumor growth while regulating glycolysis and mitochondrial functions via IGF2BPs/PRMT7 signaling. These results indicate that lncRNA PRKCQ-AS1 is a key downstream target of fasting and is involved in PTC metabolic reprogramming. Further, the PRKCQ-AS1/IGF2BPs/PRMT7 axis is an ideal therapeutic target for PTC diagnosis and treatment.
A cytosolic mutp53(E285K) variant confers chemoresistance of malignant melanoma
Authors: Dunsche Luise , Ivanisenko Nikita , Riemann Shamala , Schindler Sebastian , Beissert Stefan , Angeli Cristian , Kreis Stephanie , Tavassoli Mahvash , Lavrik Inna , Kulms Dagmar 
Published: December 2023

AbstractMalignant melanoma (MM) is known to be intrinsically chemoresistant, even though only ~20% of MM carry mutations of the tumor suppressor p53. Despite improvement of systemic therapy the mortality rate of patients suffering from metastatic MM is still ~70%, highlighting the need for alternative treatment options or for the re-establishment of conventional therapeutic approaches, including chemotherapy. Screening the p53 mutation status in a cohort of 19 patient-derived melanoma samples, we identified one rarely described missense mutation of p53 leading to E285K amino acid exchange (mutp53(E285K)). Employing structural and computational analysis we revealed a major role of E285 residue in maintaining stable conformation of wild-type p53 (wtp53). E285K mutation was predicted to cause interruption of a salt-bridge network affecting the conformation of the C-terminal helix of the DNA-binding domain (DBD) thereby preventing DNA interaction. In this context, a cluster of frequently mutated amino acid residues in cancer was identified to putatively lead to similar structural effects as E285K substitution (E285 cluster). Functional analysis, including knockdown of endogenous p53 and reconstitution with diverse p53 missense mutants confirmed mutp53(E285K) to have lost transcriptional activity, to be localized in the cytosol of cancer cells, by both means conferring chemoresistance. Re-sensitization to cisplatin-induced cell death was achieved using clinically approved compounds aiming to restore p53 wild-type function (PRIMA1-Met), or inhibition of AKT-driven MAPK survival pathways (afuresertib), in both cases being partially due to ferroptosis induction. Consequently, active ferroptosis induction using the GPX4 inhibitor RSL3 proved superior in tumorselectively fighting MM cells. Due to high prevalence of the E285-cluster mutations in MM as well as in a variety of other tumor types, we conclude this cluster to serve an important function in tumor development and therapy and suggest new implications for ferroptosis induction in therapeutic applications fighting MM in particular and cancer in general.
Activation of ILC2s through constitutive IFNγ signaling reduction leads to spontaneous pulmonary fibrosis
Authors: Otaki Natsuko , Motomura Yasutaka , Terooatea Tommy , Thomas Kelly S. , Mochizuki Miho , Takeno Natsuki , Koyasu Shigeo , Tamamitsu Miu , Sugihara Fuminori , Kikuta Junichi , Kitamura Hideya , Shiraishi Yoshiki , Miyanohara Jun , Nagano Yuji , Saita Yuji , Ogura Takashi , Asano Koichiro , Minoda Aki , Moro Kazuyo 
Published: December 2023

AbstractPulmonary fibrosis (PF), a condition characterized by inflammation and collagen deposition in the alveolar interstitium, causes dyspnea and fatal outcomes. Although the bleomycin-induced PF mouse model has improved our understanding of exogenous factor-induced fibrosis, the mechanism governing endogenous factor-induced fibrosis remains unknown. Here, we find that Ifngr1-/-Rag2-/- mice, which lack the critical suppression factor for group 2 innate lymphoid cells (ILC2), develop PF spontaneously. The onset phase of fibrosis includes ILC2 subpopulations with a high Il1rl1 (IL-33 receptor) expression, and fibrosis does not develop in ILC-deficient or IL-33-deficient mice. Although ILC2s are normally localized near bronchioles and blood vessels, ILC2s are increased in fibrotic areas along with IL-33 positive fibroblasts during fibrosis. Co-culture analysis shows that activated-ILC2s directly induce collagen production from fibroblasts. Furthermore, increased IL1RL1 and decreased IFNGR1 expressions are confirmed in ILC2s from individuals with idiopathic PF, highlighting the applicability of Ifngr1-/-Rag2-/- mice as a mouse model for fibrosis research.
Cytoglobin regulates NO-dependent cilia motility and organ laterality during development
Authors: Rochon Elizabeth R. , Xue Jianmin , Mohammed Manush Sayd , Smith Caroline , Hay-Schmidt Anders , DeMartino Anthony W. , Clark Adam , Xu Qinzi , Lo Cecilia W. , Tsang Michael , Tejero Jesus , Gladwin Mark T. , Corti Paola 
Published: December 2023

AbstractCytoglobin is a heme protein with unresolved physiological function. Genetic deletion of zebrafish cytoglobin (cygb2) causes developmental defects in left-right cardiac determination, which in humans is associated with defects in ciliary function and low airway epithelial nitric oxide production. Here we show that Cygb2 co-localizes with cilia and with the nitric oxide synthase Nos2b in the zebrafish Kupffer’s vesicle, and that cilia structure and function are disrupted in cygb2 mutants. Abnormal ciliary function and organ laterality defects are phenocopied by depletion of nos2b and of gucy1a, the soluble guanylate cyclase homolog in fish. The defects are rescued by exposing cygb2 mutant embryos to a nitric oxide donor or a soluble guanylate cyclase stimulator, or with over-expression of nos2b. Cytoglobin knockout mice also show impaired airway epithelial cilia structure and reduced nitric oxide levels. Altogether, our data suggest that cytoglobin is a positive regulator of a signaling axis composed of nitric oxide synthase–soluble guanylate cyclase–cyclic GMP that is necessary for normal cilia motility and left-right patterning.
Fragment-based drug nanoaggregation reveals drivers of self-assembly
Authors: Chen Chen , Wu You , Wang Shih-Ting , Berisha Naxhije , Manzari Mandana T. , Vogt Kristen , Gang Oleg , Heller Daniel A. 
Published: December 2023

AbstractDrug nanoaggregates are particles that can deleteriously cause false positive results during drug screening efforts, but alternatively, they may be used to improve pharmacokinetics when developed for drug delivery purposes. The structural features of molecules that drive nanoaggregate formation remain elusive, however, and the prediction of intracellular aggregation and rational design of nanoaggregate-based carriers are still challenging. We investigate nanoaggregate self-assembly mechanisms using small molecule fragments to identify the critical molecular forces that contribute to self-assembly. We find that aromatic groups and hydrogen bond acceptors/donors are essential for nanoaggregate formation, suggesting that both π-π stacking and hydrogen bonding are drivers of nanoaggregation. We apply structure-assembly-relationship analysis to the drug sorafenib and discover that nanoaggregate formation can be predicted entirely using drug fragment substructures. We also find that drug nanoaggregates are stabilized in an amorphous core-shell structure. These findings demonstrate that rational design can address intracellular aggregation and pharmacologic/delivery challenges in conventional and fragment-based drug development processes.
Directing polymorph specific calcium carbonate formation with de novo protein templates
Authors: Davila-Hernandez Fatima A. , Jin Biao , Pyles Harley , Zhang Shuai , Wang Zheming , Huddy Timothy F. , Bera Asim K. , Kang Alex , Chen Chun-Long , De Yoreo James J. , Baker David 
Published: December 2023

AbstractBiomolecules modulate inorganic crystallization to generate hierarchically structured biominerals, but the atomic structure of the organic-inorganic interfaces that regulate mineralization remain largely unknown. We hypothesized that heterogeneous nucleation of calcium carbonate could be achieved by a structured flat molecular template that pre-organizes calcium ions on its surface. To test this hypothesis, we design helical repeat proteins (DHRs) displaying regularly spaced carboxylate arrays on their surfaces and find that both protein monomers and protein-Ca2+ supramolecular assemblies directly nucleate nano-calcite with non-natural {110} or {202} faces while vaterite, which forms first in the absence of the proteins, is bypassed. These protein-stabilized nanocrystals then assemble by oriented attachment into calcite mesocrystals. We find further that nanocrystal size and polymorph can be tuned by varying the length and surface chemistry of the designed protein templates. Thus, bio-mineralization can be programmed using de novo protein design, providing a route to next-generation hybrid materials.