bluuebunny/crossref_metadata_2025_split huggingface dataset:
COVID19 vaccination prevents functional loss in elderly
Authors: Muñoz-Gutiérrez José de Jesús , Gaytán-Ramos Ángel Ariel , Rodríguez-Quintanilla Karina Alejandra 
Published: October 2023

Abstract
Background
COVID19 caused high morbidity and mortality rates among the elderly population. SARS-CoV2 vaccination significantly reduced the severity of cases, hospitalizations, and mortality. There is limited research available on the impact of vaccination on the functional performance of the elderly. This paper aims to explore the functional performance of the elderly based on their vaccination status.
Methods
This is an exploratory, observational, cross-sectional, and prospective study, conducted within a COVID-19 concentration hospital in Northern Mexico. The research focused on patients 60 years and above, who were hospitalized and tested positive for SARS-CoV-2 between March 1 and October 31, 2021. Participants were categorized based on their vaccination status upon admission. Electronic records were utilized for data collection, and a 6-month follow-up phone call, using a purpose-built instrument. The study examined mortality post-discharge, daily life activities assessed using Katz index and Lawton Brody Scale for each group.
Results
A total of 266 patients were included. 255 survived. The average age was 74 years. Regarding vaccination status, 40% were not immunized, and 31.4% had completed their immunization schedule. The analysis revealed that immunization significantly preserved IADL. The highest level of protection was observed among individuals with complete immunization status (p < 0.001; OR 0.05, 95% CI 0.01–0.18). Furthermore, complete immunization emerged as a protective factor for ADL (p = 0.008; OR 0.26, 95% CI 0.09–0.71).
Conclusion
The key finding: even incomplete vaccinations prevent functional loss in the elderly. Our study underscores the need to remove barriers hindering elderly vaccination.
Inhibition of VCAM-1 gene transcriptional blockade by RHOdiroside through inhibition of P65 and CSN5 deubiquitination reduces atherosclerosis in monocytes and endothelial adherent cells
Authors: Zhang Xu , Tang WenLian , Liu Xingquan , Luo Lin , Xin Xin , Ju Nana , Xiong Huarong , Wu Ping , Zhang Xian , Zhang Daiwei , Yu Lan , Li Gen , Zhao Feipeng , Wang Jianing , Ji Jing , Liu chao 
Published: October 2023

Abstract
Monocyte-endothelial cell adhesion plays a crucial role in the early development of atherosclerosis, contributing to lipid disruption and exacerbating the condition. RHOdiola (RHO), a prominent Chinese medicinal drug, possesses diverse pharmacological activities such as anti-inflammatory, antioxidant, anti-cancer, anti-metabolic deregulation, and neuroprotective effects. However, the specific anti-atherosclerotic effects of RHO remain incompletely understood. Thus, this study aimed to investigate the potential beneficial impact of RHO on atherosclerosis. ApoE-/- mice were fed a high-fat diet and administered RHO treatment. Protein expression levels of GATA2, CSN5, and VCAM-1 in the aortic endotheliμM were evaluated. Our findings demonstrate a reduction in GATA2, CSN5, and VCAM-1 protein expression levels in the aortic endotheliμM, accompanied by decreased P65 phosphorylation levels. Additionally, GATA2 ubiquitination was downregulated, The binding strength of PP2AC and I2PP2A decreased while its binding to P65 increased. Moreover, RHO directly inhibited the transcriptional activity of NF-κB subunit P65 as a transcription factor by targeting the I2PP2A-PP2Ac axis. Furthermore, it interacted with the transcription factor GATA-2 through CSN5-mediated deubiquitination, thereby directly suppressing the transcription of the P65-regulated VCAM-1 gene. In conclusion, the combined dual inhibition of VCAM-1 gene transcription, along with the consequent reduction in monocyte-endothelial cell adhesion, mediates the anti-atherosclerotic biological activity exerted by RHO.
Mechanistic understanding of the "gatekeeper" phosphofructokinase of the glycolytic pathway on the central carbon metabolism
Authors: Li Lingyun , Qin Ning , Chen Xin , Chen Yu , Ji Xu , Zhang Yijie , Nielsen Jens , Li Zihe 
Published: October 2023

Abstract
Background: Glycolysis is one of the oldest and most fundamental metabolic pathways responsible for glucose breakdown and energy production in cells. The key regulatory enzyme in glycolysis is phosphofructokinase (Pfks), which catalyzes the phosphorylation of fructose 6-phosphate (F6P) to fructose 1,6-bisphosphate. The regulation of Pfks plays a crucial role in fine-tuning glycolytic flux to meet the cellular energy demands, making it a "gatekeeper" enzyme of glycolysis. In Saccharomyces cerevisiae, Pfks has evolved into a hetero-octameric phosphofructokinase, consisting of the Alpha and Beta subunits encoded by PFK1 and PFK2, respectively. Deletion of either PFK1 or PFK2 could significantly impair the organism's fitness, whereas the underlying metabolic mechanisms remain unclear. In this study, we investigated the specific effects of PFK1 and PFK2 on the cellular metabolism of S. cerevisiae.
Results: Deletion of either PFK1 or PFK2 significantly reduced the cell growth, decreased ethanol and glycerol production and increased acetate production. Furthermore, through Flux Balance Analysis (FBA) and transcriptome analysis, we observed a significant upregulation of the Tricarboxylic Acid (TCA) cycle and the electron transfer chain in the PFK2 deletion strain. This upregulation indicated a shift towards enhanced oxidative respiration and energy production under the absence of the Beta subunit of Pfks. Furthermore, we demonstrated that the deletion of PFK2 led to a 33% improvement in the free fatty acid production, indicating its effectiveness as a key factor in regulation of lipid metabolism as well as bioproduction.
Conclusions: Our physiological and transcriptomic data suggested that, as an isoenzyme, Pfk1 has a relatively limited influence on the metabolism of S. cerevisiae, whereas Pfk2 plays significant and vital roles in cellular metabolism. Deletion of PFK2 has the potential to greatly enhance respiration in yeast under aerobic conditions. These observations underscore the pivotal role of PFK2 in governing cellular energy metabolism and suggest that manipulation of PFK2 expression could hold promising implications for bioproduction processes.
2-approximation algorithm for soft-capacitated facility location problem with linear penalties
Authors: Zhu Lihao , Qiu Xiangyan , Li Qiaoliang 
Published: October 2023

Abstract
In this paper, we will be discussing an extension of the classical facility location problem known as the soft-capacitated facility location problem with linear penalties. In this problem, some demand points are allowed to be abandoned under the condition of paying them established linear penalties. Each facility has its own inherent capacity limit, but each facility can be opened multiple times, just paying a corresponding multiple of the opening cost. This problem is a generation of the uncapacitated facility location with linear penalties. To solve this problem, we present a 2-approximation algorithm by reduction technique, and prove that the algorithm achieves the integrality gap of its standard linear programming relaxation. This means that if the value of the objective function corresponding to the optimal solution of the standard linear relaxation of the soft-capacitated facility location problem with linear penalties is used as the lower bound, a better approximation ratio will not be obtained.
Is metformin associated with a lower prevalence of polyps, adenomas and colorectal carcinoma in patients with diabetes mellitus?
Authors: Canha Maria Inês , Ramos Gonçalo , Prata Rita , Martins Pedro Lages , Ramos Marta Viúla , Coimbra João 
Published: October 2023

Abstract

Purpose. Recent studies suggested a protective role of metformin in the development of colorectal cancer (CRC) and its precursors. We aimed to investigate if metformin was associated with a lower prevalence and number of colorectal polyps in diabetic patients and, secondarily, adenomas, high-risk adenomas and CRC.Methods. Retrospective study on adult patients with diabetes mellitus followed in our hospital with a total colonoscopy between 2015–2019, treated with either metformin for > 5 years or other antidiabetic agent (control group). We assessed the number, size and histology of proliferative lesions detected on colonoscopy.Results. We included 401 patients aged 69 ± 9 years, 57% males, divided in two groups: treated with metformin (n = 260) and without (n = 140). The number of polyps detected was significantly lower in patients under metformin (p = 0.014). There was a nonsignificant trend towards lower polyp detection rates in the metformin compared to the control group both in unadjusted analysis (50% vs 60%, p = 0.058) and adjusted analysis (odds ratio [OR] 0.68, 95% confidence interval [CI] 0.43–1.09, p = 0.111). In the latter we identified male gender (OR 2.24, 95%CI 1.44–3.49, p < 0.001), age (OR 1.35 for every 10 years, 95%CI 1.07–1.71, p = 0.012), glycated hemoglobin value (OR 1.20 for every 1% increase, 95%CI 1.06–1.37, p = 0.005) and hypertension (OR 1.76, 95%CI 1.01–3.08, p = 0.046) as factors associated with a higher prevalence of polyps. We saw no statistically significant differences regarding adenoma (p = 0.231), high-risk adenoma (p = 0.810) and CRC (p = 0.705) diagnoses between groups.Conclusion. In our study, metformin was associated with less colorectal polyps in diabetic patients compared to other treatment modalities. The polyp detection rates, although lower in the metformin group, had no statistically significant differences between groups both in unadjusted and adjusted analysis.
Impact of Delayed Removal of Pectus Bar on Outcomes Following Nuss Repair: A Retrospective Analysis
Authors: Keong Der-En , Tzeng I-Shiang , Htut Nay , Fan Yu-Jiun , Hsieh Min-Shiau , Cheng Yeung-Leung 
Published: October 2023

Abstract
Background
Usually, pectus bars are removed 3 years after Nuss procedure in patients with pectus excavatum. However, the optimal timing for postoperative pectus bar removal remains undefined. Our study investigated the effects of delayed pectus bar removal after Nuss repairs.
Methods
Retrospective data were collected on patients who underwent Nuss procedures for pectus excavatum and had their bars removed from August 2014 to December 2020. Patients with correction period > 3 years were divided into group A (< 6 years) and group B (≥ 6 years). Propensity score matching was used to compare complications and radiological outcomes associated with bar removal.
Results
Of the 542 patients who underwent bar removal, 451 (Group A: 419 patients, Group B: 32) had correction duration > 3 years. The average correction duration was 4.5 ± 1.4 years. After propensity score matching analysis, group B (mean: 8.0 (6.0–16.2) years) exhibited significantly longer median operative times (85 vs 55 min; P = 0.026), higher callus formation rates (68.8% vs 46.9%; P = 0.029), and greater median intraoperative blood loss (35 vs 10 mL; P = 0.017) than group A (mean: 4.2 (3.0–5.9) years). However, following bar removal, the groups showed no statistical differences in the surgical complication rates (group A: 6.3% vs group B: 9.4%; P = 0.648) or median ratio of radiological improvement (an improvement on the Haller index on chest radiograph, 21.0% vs 22.2%; P = 0.308).
Conclusions
Delaying pectus bar removal after Nuss repair presents certain challenges but does not compromise overall outcomes. These findings suggest that a longer correction period may be unnecessary. However, further multicenter studies with long-term follow-up are warranted to assess long-term outcomes.
Study on the dynamic adsorption and recycling of phosphorus by Fe-Mn oxide/mulberry branch biochar composite adsorbent
Authors: Meina Liang , Qiao Mushi , Zhang Qing , Xu Shuiping 
Published: October 2023

Abstract
In this study, the Fe-Mn oxide/mulberry stem biochar composite adsorbent (FM-MBC) was used to conduct dynamic adsorption experiments on phosphate in water. The effects of pH value (3.0, 4.5, and 6.0), initial concentration of phosphorus (10, 20, and 30 mg L− 1), adsorbent bed height (2, 3, and 4 cm), and solution flow rate (1, 2, and 3 mL min− 1) were investigated to analyze the breakthrough curves. The results showed that as the initial phosphorus concentration and flow rate increased, and the bed height decreased, the breakthrough time was shortened. This indicates that higher initial phosphorus concentrations and flow rates, as well as lower bed heights, led to faster breakthrough of phosphate ions in the FM-MBC adsorbent. Additionally, it was observed that increasing the pH value was not conducive to the adsorption of phosphorus by the FM-MBC adsorbent. To analyze the experimental data, three different models [Yoon Nelson, Thomas, Adams-Bohart and Bed depth service time (BDST)] were fitted to the dynamic adsorption data under different experimental conditions. These models were used to predict the breakthrough curves and evaluate the model parameters of the fixed bed for process design reference. Among the four models, the Thomas model showed good agreement with the experimental data, indicating its suitability for describing the dynamic adsorption process of phosphate ions using FM-MBC. The saturated fixed-bed column including FM-MBC were regenerated with NaOH and HCl, which 0.3 mol L− 1 NaOH solution had the best regeneration effect. Overall, the results of the study demonstrated that FM-MBC has excellent adsorption properties for removing phosphate from aqueous solutions.
Alterations of the fractional amplitude of low-frequency fluctuations in patients with Moyamoya disease after revascularization: a resting-state functional magnetic resonance imaging study
Authors: Qi Yi-Chen , Deng Yu-En , Wen Jia-Yi , Chen Lu-Jia , Wu Di , Wu Shi-Nan , Shao Yi 
Published: October 2023

Abstract
Objective: Revascularization surgery is commonly used to treat Moyamoya disease (MMD). To assess the effects of revascularization, we investigated the neural activities in patients with MMD using the alterations of the fractional amplitude of low-frequency fluctuations (fALFF).
Methods: Eighteen patients with MMD (6 male and 12 female) who had undergone revascularization surgery and 18 healthy controls of the same sex were selected for the study. The fALFFs in the brain regions of all the participants were investigated and analyzed.
Results: The fALFF of the Right Medial Frontal Gyrus, Frontal_Inf_Orb_R, Frontal_Inf_Tri_R, Frontal_Sup_L, and Right Medial Frontal Gyrus increased after the surgical intervention. The fALFF in Thalamu_R, Hippocampus_L, and Cerebellum_L decreased. However, the alternations of mean ALFF values in the different regions did not correlate with postoperative clinical manifestations in MMD patients.
Conclusion: Surgical interference can improve brain function in patients with MMD, and fALFF can be used for more accurate predictions of the prognosis of MMD.
Comparison in radiographic outcomes between robotic-assisted cruciate- retaining and posterior-stabilized total knee arthroplasty: a prospective randomized controlled study
Authors: Huang Zhenchao , Zhang Zian , Yu Chaoqun , Wang Wenzhe , Zhang Haining 
Published: October 2023

Abstract
Background
Robotic-assisted total knee arthroplasty (RA-TKA) is currently increasingly used in the field of joint arthroplasty, with cruciate-retaining (CR) and posterior-stabilized (PS) prostheses currently being the most widely used prosthesis designs. The purpose of this study was to investigate the difference in radiographic outcomes and early clinical outcomes between CR and PS in RA-TKA.
Methods
This prospective randomized controlled study enrolled 120 patients who underwent primary CR or PS RA-TKA between July 2022 and March 2023. Knee radiographs were taken one-day postoperative, while range of motion (ROM) and clinical scores were recorded in the outpatient clinic six-month postoperative. Radiographic outcomes and early clinical outcomes between the two groups were compared, respectively.
Results
Patients implanted with PS achieved a lower postoperative PTS (P < 0.001), as well as a higher ΔFTA (P = 0.001), ΔHKA (P < 0.001) and absolute ΔPTS (P < 0.001) compared to patients with CR RA-TKA. All radiographic parameters in both groups were changed significantly after surgery (P < 0.001). No difference was found in Forgotten Joint Score-12 (FJS-12) or 5-point Likert Score. However, postoperative ROM (P = 0.034) and ΔROM (P = 0.012) was significantly higher in CR than in PS group.
Conclusion
Patients with PS can achieve a lower postoperative PTS, and a higher ΔFTA, ΔHKA and absolute ΔPTS compared to patients with CR RA-TKA. Postoperative ROM and ΔROM were higher in CR than in PS group. However, the significant difference in radiographic outcomes and ROM did not translate into any difference in clinical outcomes. No difference was found in FJS-12 or 5-point Likert Score.
A Traffic Flow Prediction Method Based on Blockchain and Federated Learning
Authors: Zhi Hui , 段 苗苗 , Yang Lixia 
Published: October 2023

Abstract
Traffic flow prediction is the an important issue in the field of intelligent transportation, and real-time and accurate traffic flow prediction plays a crucial role in improving the efficiency of traffic networks. Existing traffic flow prediction methods use deep learning models and collected traffic flow datasets to predict traffic flow. These datasets contain the private data of clients, so if some clients are unwilling to participate in the traffic flow prediction, the traffic flow prediction results will be inaccurate. Therefore, it is important to address the issue that how to motivate clients to actively participate in the traffic flow prediction while protecting the privacy data. So, this paper proposes a traffic flow prediction method based on blockchain and federated learning (TFPM-BFL). Firstly, the traffic flow prediction problem is described as federated learning (FL) task, the improved long and short-term memory (LSTM) model is used to predict the traffic flow at the client side, the traffic flow data is decomposed by wavelet function, and the LSTM network with added attention mechanism is used to obtain traffic flow prediction results; Then, incentive mechanism based on reputation value is proposed, the model parameters are uploaded to the blockchain for local and partial reputation evaluation through smart contracts, and the corresponding global reputation update is obtained, the reward is distributed to clients according to global reputation, so the clients are motivated to participate in the traffic flow prediction; Finally, the model aggregation method based on reputation value and compression rate is designed. Based on the reputation evaluation results, the edge server uses the Topk algorithm to perform high-quality aggregation of the local model parameters uploaded by clients (roadside units), central server aggregates the partial model parameters from edge server, and then the central server distributes the global aggregated model parameters to clients to perform the next round of FL. By using the FL framework, TFPM-BFL uploads the model parameters instead of the original traffic flow data, so it can protect private data. Moreover, it can provide incentive mechanism through reputation evaluation and reward to encourage clients to participate in the FL task. Simulation results show that TFPM-BFL can realize accurate and timely traffic flow prediction, and it can effectively motivate clients to participate in FL task while ensuring the privacy of the underlying data.
Molecular Characterization of the Multi-drug Resistant Myroides Odoratimimus Isolates: a Whole Genome Sequence-based Study to Confirm Carbapenem Resistance
Authors: Yartaşı Esra , Durmaz Rıza , Arı Oğuz , Mumcuoğlu İpek , Dinç Bedia 
Published: October 2023

Abstract
The bacteria belonging to the Myroides genus are opportunistic pathogens causing community or hospital-acquired infections that result in treatment failure due to antibiotic resistance. This study aimed to investigate molecular mechanisms of antibiotic resistance, clonal relatedness, and the biofilm forming capacity of the 51 multi-drug resistant Myroides odoratimimus. All isolates were screened for blaKPC, blaOXA, blaVIM, blaIMP, blaMUS, blaTUS, blaNDM, and blaB genes by using PCR amplification. Whole genome sequencing (WGS) was applied on three isolates for further investigation of antibiotic resistance mechanisms. Clonal relatedness was analyzed by Pulsed-field gel electrophoresis (PFGE) and the microtiter plate method was used to demonstrate biofilm formation. All isolates were positive for biofilm formation. PCR analysis resulted in a positive for only the blaMUS-1 gene. WGS identified blaMUS-1, erm(F), ere(D), tet(X), and sul2 genes in all strains tested. PFGE yielded a clustering rate of 96%. High clonal relatedness, biofilm formation, and multi-drug resistance properties may lead to the predominance of these opportunistic pathogens in hospital environments and make them cause nosocomial infections.
mmu-miR-374b-5p modulated inflammatory factors via downregulation of C/EBP β/NF-κB signaling in Kupffer cells during Echinococcus multilocularis infection
Authors: Pu Guiting , Li Yanping , Liu Tingli , Li Hong , Wang Liqun , Chen Guoliang , Amuda Tharheer Oluwashola , Guo Xiaola , Luo Xuenong 
Published: October 2023

Abstract
Background
Alveolar echinococcosis (AE) is an important infectious disease caused by the metacestode larva of Echinococcus multilocularis (E. multilocularis), seriously threatening global public health security. Kupffer cells (KCs) play important roles in inflammasome. However, its role in hepatic alveolar echinococcosis not yet fully elucidated.
Methods
In this study, qRT-PCR was used to detect the expression level of miR-374b-5p in KCs. C/EBP β, one of the targets of miR-374b-5p, was identified through luciferase reporter assays and loss-of-function and gains. Critical genes involved in NFκB signaling pathway were analyzed by qRT-PCR and western blot.
Results
This study reported that miR-374b-5p was significantly up-regulated in KCs during E. multilocularis infection, and further showed that miR-374b-5p was able to bind to the 3'-UTR of the C/EBP β gene and regulated its expression. The expression levels of NF-κBp65 and p-NF-κBp65 and pro-inflammatory factor including iNOS, TNFα, and IL6 was attenuated after overexpression of miR-374b-5p while enhanced after suppression of miR-374b-5p. However, the Arg1 expression level was promoted after overexpression of miR-374b-5p while suppressed after suppression of miR-374b-5p. Additionally, the increased protein levels of NF-κBp65 and p-NF-κBp65 were found in the C/EBP β-overexpressed KCs.
Conclusions
These results demonstrated that miR-374b-5p probably regulated the expression of inflammatory factors via C/EBP β/NF-κB signaling. This finding is helpful to explore the mechanism of inflammation regulation during E. multilocularis infection.