bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sexual quality of life between healthy women and women with gynecological cancer: Results of a hospital‐based case–control study in Türkiye
Authors: Ören Ekin Dila Topaloğlu , Kiziltaş Selin 
Published: December 2023

AbstractBackgroundGynecological cancers among women were both chronic and vitally health problems. The increasing prevalence of women with surviving gynecological cancers and the receiving treatments of cancer negatively affected the sexual quality of life.AimThe study was conducted to determine the sexual quality of life of healthy women and women with gynecological cancer and to determine the predictors of sexual quality of life in women with gynecological cancer.MethodsThe research is a hospital‐based case–control study was conducted. The study was carried out with 65 patients with histologically confirmed gynecological cancer were compared with 75 controls, who were admitted to the different departments of the same hospital in the Izmir, in western Türkiye.ResultsThe Sexual Quality of Life Questionnaire score (SQOL) of cases was 60.12 ± 9.01 and controls was 78.92 ± 7.08. SQOL scores of cases were significantly lower than controls (p < 0.05). Multiple linear regression analysis results showed that age (B = −0.295, p = 0.072), working (B = 4.981, p = 0.016), smoking (B = −4.590, p = 0.035), diagnosed with cervical cancer (B = −4.214, p = 0.045), and receiving chemotherapy (B = −4.722, p = 0.028) were associated with sexual quality of life in women with gynecological cancer (p < 0.05, R2: 0.40).ConclusionWomen with gynecological cancer had lower quality of sex life compared with healthy women. Gynecological cancers negatively affect women's sexual quality of life. Health professionals (especially nurses) should be aware of the potential problems that women with gynecological cancer may experience with their sexual lives. They should develop coping strategies and offer solutions for the problems experienced by women with gynecological cancer.
Adverse outcomes in subsequent pregnancies in women with history of recurrent spontaneous abortion: A meta‐analysis
Authors: Jia Dan , Sun Fengdan , Han Sisi , Lu Lijuan , Sun Yuanyuan , Song Qingxia 
Published: December 2023

AbstractObjectiveThis study aimed to investigate the association between a history of recurrent spontaneous abortion (RSA) and adverse outcomes in women with spontaneous conception.MethodsA search strategy from the inception to March 3, 2023 was run in PubMed, Embase, Cochrane Library, and Web of Science databases. The odds ratio (OR), and the 95% confidence interval (CI) or point estimation were used as the evaluation indexes. Each outcome measure tested was assessed for heterogeneity using the Cochran Q test. Sensitivity analyses were performed to test the credibility of the meta‐analysis results.ResultsFifteen studies involving 1 475 389 pregnant women were included. A history of RSA was associated with gestational diabetes (OR: 2.21, 95% CI: 1.70–2.87, p < 0.001), preeclampsia (OR: 2.06, 95% CI: 1.49–2.86, p < 0.001), placenta previa (OR: 1.82, 95% CI: 1.09–3.02, p = 0.021), placental abruption (OR: 1.67, 95% CI: 1.36–2.06, p < 0.001), miscarriage (OR: 6.37, 95% CI: 3.83–10.57, p < 0.001), preterm birth (OR: 1.80, 95% CI: 1.36–2.37, p < 0.001), cesarean section (OR: 1.47, 95% CI: (1.13–1.91, p = 0.004), perinatal death (OR: 2.24, 95% CI: 1.39–3.60, p = 0.001), and neonatal intensive care unit admission (OR: 1.39, 95% CI: 1.01–1.92, p = 0.047). However, the associations of a history of RSA with gestational hypertension, small for gestational age, fetal anomalies, fetal growth restriction, and postpartum hemorrhage were not observed.ConclusionThis meta‐analysis indicates a history of RSA was associated with increased risks of several adverse outcomes in pregnant women with spontaneous conception.
Preliminary study of HPV integration status on the occurrence and development of vaginal intraepithelial neoplasia
Authors: Yuan Shuning , Qian Cheng , Zhang Hailong , Xing Yan 
Published: December 2023

AbstractAimThe aim of this study was to investigate how the integration status of HPV in the vaginal epithelium affects the development of vaginal intraepithelial neoplasia (VaIN).MethodsTwenty‐four vaginal tissues were collected before applying high‐throughput viral integration detection (HIVID), medical records of them were documented, including age, thin‐prep cytologic test (TCT) and HPV test results, colposcopic biopsy pathology, and other clinical data, such as history of total hysterectomy for cervical lesions, whether they were infected with HPV16/18 with a follow‐up span of 2 years. We summarized the distribution of HPV integration on the host chromosome and HPV type, as well as the hotspot integration gene and its role in the development of VaIN.ResultsIn this study, 24 cases suffered from VaIN were involved. HPV integration was detected in 11 cases; furthermore, we discovered HPV 16 and 73, chromosome 1 and 2 possessed most HPV integration sites while EMBP1, CLO5A1, EHF, ELF5 as dominate hot spots. Taken clinical outcome into account, we found a significant difference between HPV integration occurrence and VaIN (p = 0.011).Conclusion(1) This study found a statistical difference between HPV integration and the occurrence of VaIN; (2) HPV integration may provide a new clinical predictor for VaIN and facilitate risk assessment and stratified management of high‐risk patients.
Going the Extra Mile: How High School Staff Use Informal Strategies to Support, Protect, and Care for LGBTQ+ Students*
Authors: Dougherty Michelle , Plenn Eion R. , Corey Stephanie L. , Onufer Lindsay , Coulter Robert W. S. ,   Coulter Lab Qualitative Team
Published: December 2023

ABSTRACTBACKGROUNDHigh school staff can play a key role in supporting students with LGBTQ+ identities through informal strategies; however, more research is needed to understand how staff are employing these strategies.METHODSWe conducted semi‐structured interviews, collecting information on informal strategies used to support students identifying as LGBTQ+ from a diverse sample of 23 school staff from high schools across the United States.RESULTSStaff employed informal support strategies across social ecological levels: within the school community, staff interacted with parents/guardians of students and advocated for more inclusive policies. Within the classroom, staff created inclusive physical environments, implemented inclusive curricula, and employed bullying prevention strategies. Interpersonally, staff listened to and affirmed students and collaborated to employ support strategies. Intrapersonal qualities, including having a personal connection to the LGBTQ+ community and demonstrating open‐mindedness, facilitated staff efforts to support students.CONCLUSIONSSupporting staff in the implementation of the strategies we identified could foster more inclusive school environments, advancing equity for students identifying as LGBTQ+.
Natural history of symptoms and prognostic information of the rapid drink challenge and solid bolus swallows in esophagogastric junction outflow obstruction defined by manometry
Authors: Josefsson Axel , Simrén Magnus , Smolak Adam , Sabbagh Nour , Törnblom Hans 
Published: December 2023

AbstractBackground/IntroductionEsophagogastric junction outflow obstruction (EGJOO) is a condition characterized by poor relaxation of the lower esophageal sphincter (LES), which can manifest as dysphagia and chest pain. The best treatment of EGJOO is unknown as some patients improve without any specific therapy, whereas some patients undergo invasive therapy. Currently, prognostic factors are lacking. We aimed to assess the long‐term prognosis and predictors of dysphagia and chest pain by the rapid drink challenge and solid bolus swallows in EGJOO.MethodsWe retrospectively assessed high‐resolution esophageal manometries (HRM) performed at our center between 2015 and 2018. The patients completed a dysphagia and chest pain questionnaire a median of 34 months after the HRM/baseline assessment, including the Impaction dysphagia questionnaire‐10 (IDQ‐10) complemented with questions regarding chest pain and esophageal treatments. Symptoms were compared with HRM findings.ResultsIn all, 980 HRMs were analyzed and 66 (6.5%) were identified as having HRM findings compatible with EGJOO. Of these, 27 patients with EGJOO (41%) completed the follow‐up questionnaires and had no exclusion criteria, and 70% of these patients had dysphagia and 44% chest pain at least once a week. Dysphagia at follow‐up was more common in patients with elevated integrated relaxation pressure (IRP) on all three HRM metrics (water swallows, solid bolus swallows, and rapid drink challenge) (p = 0.03, odds ratio: 8.4 (95% CI: 1.2–56.0)), but this was not seen for chest pain (p = 0.45). Abnormal motility patterns on rapid drink challenge or solid bolus swallows were not associated with dysphagia or chest pain at follow‐up.ConclusionsHaving a high IRP on three HRM metrics—water swallows, solid bolus swallows, and rapid drink challenge—is associated with a worse prognosis in patients with EGJOO and could potentially be used to select candidates suitable for invasive procedures.
A global study of screening intensity and economic status on epidemic control performance during various epidemic periods of COVID‐19 mutant strains
Authors: Chang Chao‐Chin , Chang Chia‐Lin 
Published: December 2023

AbstractThis study analyzed global data on epidemic control measures and economic conditions in different countries during different mutant strain epidemic periods, including the Alpha, Delta, and Omicron strains. The study estimated the elasticity coefficient through a log‐log model, which represents the percent change of the confirmed case number with respect to a percent change in the total number of screening tests in a country for epidemic control. The 7‐day rolling data of screening tests and confirmed cases from the Our World in Data database for the pandemic periods of Alpha strain in 2020, Delta strain in 2021, and Omicron strain in 2022, suggest that the magnitude of the elasticity was associated with the economic condition of a country. Compared with the results during either Alpha or Delta pandemic period, the Omicron pandemic has a much higher estimated elasticity coefficient of 1.317 (Alpha: 0.827 and Delta: 0.885). Further examining economic conditions categorized by quartile ranges, the results indicate that the elasticity is statistically significantly lower in countries with gross domestic product (GDP) per capita between $11,354 and $26,651, and in countries with GDP per capita above $26,651 than in countries with GDP per capita below $3,335. These results suggest that countries should consider not only epidemiological measures but also economic conditions when formulating epidemic control strategies. This study highlights the importance of assessing the appropriateness of epidemic control strategies within a country and provides valuable insights into the effectiveness of such strategies, particularly in the context of community screening.
Open trial of recombinant Der f 2 pullulan‐conjugated immunotherapy in cats
Authors: Martini Franco , Rostaher Ana , Favrot Claude , Fischer Nina M. 
Published: December 2023

AbstractBackgroundAllermmune HDM (Zenoaq) is a recombinant Dermatophagoides farinae 2 (Der f 2) pullulan‐based immunotherapy vaccine whose efficacy on house dust mite allergic dogs has been demonstrated. There is no published information on its use in cats.ObjectivesThe objective of the study was to evaluate the safety and short‐term effects of Allermmune HDM in Dermatophagoides farinae (Df)‐sensitised cats.Materials and MethodsEleven cats diagnosed with atopic skin syndrome received Allermmune weekly for six weeks then monthly for three months (total duration 18 weeks). On Weeks 0, 6 and 18 clinical lesions were assessed by the Feline Dermatitis Extent and Severity Index (FEDESI); owners assessed pruritus with a 10‐cm Visual Analog Scale (pVAS). Concurrent medication use was recorded. The allergen‐specific immunoglobulin (Ig)E were measured before study inclusion with a commercial serological assay.ResultsThere were no evident adverse effects. FEDESI and pVAS improved significantly after six weeks (p = 0.001 and p = 0.01, respectively). The pretreatment Df‐specific IgE levels were significantly higher in the cats with improved clinical scores than in the cats with no clinical score change (p = 0.009).Conclusions and Clinical RelevanceAllermmune HDM may be safe in cats and has the potential to alleviate signs of atopic skin syndrome. Allergen‐specific IgE levels may represent an efficacy marker. Controlled studies of longer duration and larger sample size are worth pursuing.
In vivo
dose response and efficacy of the β-lactamase inhibitor, durlobactam, in combination with sulbactam against the
Acinetobacter baumannii-calcoaceticus
complex
Authors: O'Donnell John , Tanudra Angela , Chen April , Newman Joseph , McLeod Sarah M. , Tommasi Rubén 
Published: December 2023

ABSTRACT

Multi-drug resistant (MDR)
Acinetobacter baumannii
is emerging as a pathogen of increasing prevalence and concern. Infections associated with this Gram-negative pathogen are often associated with increased morbidity and mortality and few therapeutic options. The β-lactamase inhibitor sulbactam used commonly in combination with ampicillin demonstrates intrinsic antibacterial activity against
A. baumannii
acting as an inhibitor of PBP1 and PBP3, which participate in cell wall biosynthesis. The production of β-lactamases, particularly class D oxacillinases, however, has limited the utility of sulbactam resorting to increased doses and the need for alternate therapies. Durlobactam is a non-β-lactam β-lactamase inhibitor that demonstrates broad β-lactamase inhibition including class D enzymes produced by
A. baumannii
and has shown potent
in vitro
activity against MDR
A. baumannii
, particularly carbapenem-resistant isolates in susceptibility and pharmacodynamic model systems. The objective of this study is to evaluate the exposure-response relationship of sulbactam and durlobactam in combination using
in vivo
neutropenic thigh and lung models to establish PK/PD exposure magnitudes to project clinically effective doses. Utilizing established PK/PD determinants of %T>MIC and AUC/MIC for sulbactam and durlobactam, respectively, non-linear regressional analysis of drug exposure was evaluated relative to the 24-hour change in bacterial burden (log
10
CFU/g). Co-modeling of the data across multiple strains exhibiting a broad range of MIC susceptibility suggested net 1-log
10
CFU/g0 reduction can be achieved when sulbactam T>MIC exceeds 50% of the dosing interval and durlobactam AUC/MIC is 10. These data were ultimately used to support sulbactam-durlobactam dose selection for Phase 3 clinical trials.
Liposomal formulation of a new antifungal hybrid compound provides protection against
Candida auris
in the
ex vivo
skin colonization model
Authors: Jaromin Anna , Zarnowski Robert , Markowski Adam , Zagórska Agnieszka , Johnson Chad J. , Etezadi Haniyeh , Kihara Shinji , Mota-Santiago Pablo , Nett Jeniel E. , Boyd Ben J. , Andes David R. 
Published: December 2023

ABSTRACT

The newly emerged pathogen,
Candida auris
, presents a serious threat to public health worldwide. This multidrug-resistant yeast often colonizes and persists on the skin of patients, can easily spread from person to person, and can cause life-threatening systemic infections. New antifungal therapies are therefore urgently needed to limit and control both superficial and systemic
C. auris
infections. In this study, we designed a novel antifungal agent, PQA-Az-13, that contains a combination of indazole, pyrrolidine, and arylpiperazine scaffolds substituted with a trifluoromethyl moiety. PQA-Az-13 demonstrated antifungal activity against biofilms of a set of 10 different
C. auris
clinical isolates, representing all four geographical clades distinguished within this species. This compound showed strong activity, with MIC values between 0.67 and 1.25 µg/mL. Cellular proteomics indicated that PQA-Az-13 partially or completely inhibited numerous enzymatic proteins in
C. auris
biofilms, particularly those involved in both amino acid biosynthesis and metabolism processes, as well as in general energy-producing processes. Due to its hydrophobic nature and limited aqueous solubility, PQA-Az-13 was encapsulated in cationic liposomes composed of soybean phosphatidylcholine (SPC), 1,2-dioleoyloxy-3-trimethylammonium-propane chloride (DOTAP), and
N
-(carbonyl-methoxypolyethylene glycol-2000)-1,2-distearoyl-
sn
-glycero-3-phosphoethanolamine, sodium salt (DSPE-PEG 2000), and characterized by biophysical and spectral techniques. These PQA-Az-13-loaded liposomes displayed a mean size of 76.4 nm, a positive charge of +45.0 mV, a high encapsulation efficiency of 97.2%, excellent stability, and no toxicity to normal human dermal fibroblasts. PQA-Az-13 liposomes demonstrated enhanced antifungal activity levels against both
C. auris
in
in vitro
biofilms and
ex vivo
skin colonization models. These initial results suggest that molecules like PQA-Az-13 warrant further study and development.
Comparative evolution of influenza A virus H1 and H3 head and stalk domains across host species
Authors: Trovão Nidia S. , Khan Sairah M. , Lemey Philippe , Nelson Martha I. , Cherry Joshua L. 
Published: December 2023

ABSTRACT
The influenza A virus hemagglutinin protein (HA) has been studied extensively in humans but less so in other host species. Here, we compared the rates of nucleotide substitution, protein evolution, and glycosylation in the H1 and H3 head and stalk domains among five host classes (avian, canine, equine, human, and swine). For further resolution, we separately analyzed 49 lineages differentiated by host and geography. HA evolution in non-human hosts was more dynamic than expected. For example, the classical swine H1 head domain accumulated glycosylation sites in the antigenically important head domain at a rate as high as in humans. However, whereas the stalk domain was highly conserved in humans, with a low ratio of non-synonymous to synonymous changes, this ratio was approximately 2.5-fold higher in canines. Together, these findings highlight the need for further study of HA evolution in non-human hosts.

IMPORTANCE
For decades, researchers have studied the rapid evolution of influenza A viruses for vaccine design and as a useful model system for the study of host/parasite evolution. By performing an exhaustive analysis of hemagglutinin protein (HA) sequences from 49 lineages independently evolving in birds, swine, canines, equines, and humans over the last century, our work uncovers surprising features of HA evolution. In particular, the canine H3 stalk, unlike human H3 and H1 stalk domains, is not evolving slowly, suggesting that evolution in the stalk domain is not universally constrained across all host species. Therefore, a broader multi-host perspective on HA evolution may be useful during the evaluation and design of stalk-targeted vaccine candidates.
Dimeric immunoglobulin A as a novel diagnostic marker of measles infection
Authors: Mohd Hanafiah Khayriyyah , Hiebert Joanne , Zubach Vanessa , Severini Alberto , Anderson David A. , Drummer Heidi E. 
Published: December 2023

ABSTRACT

Despite tremendous measles incidence reduction through universal vaccination, elimination efforts rely on improved surveillance. The detection of anti-measles immunoglobulin M (IgM) by enzyme-linked immunosorbent assay is the standard laboratory diagnostic method. However, true infection is rare, and seroconversion following measles, mumps, and rubella vaccination also generates IgM, which results in low positive predictive values of assays in elimination settings, thus necessitating confirmatory testing. Improved diagnostic tests for measles infection are a World Health Organization research priority. We investigated whether dimeric immunoglobulin A (dIgA), the predominant antibody produced in mucosal immunity, may be a marker of recent or acute measles infection. We examined a serological panel of confirmed measles infection (anti-measles IgM positives,
n
= 50) and non-measles infection with rubella (
n
= 36), roseola (
n
= 40), chikungunya/dengue/zika (
n
= 41), parvovirus (
n
= 35), and other fever-rash illnesses of unknown cause (
n
= 37). Sera were examined on microimmune anti-measles IgM, Euroimmun anti-measles virus lysate (VL), and nucleoprotein (NP) IgM kits. Assays were then modified to detect dIgA using an in-house protocol based on a recombinant chimeric secretory component protein and an anti-secretory component monoclonal antibody. We observed significantly higher levels of anti-measles VL dIgA in measles samples than in non-measles controls (
P
< 0.001), and there was a low correlation with IgM (R
2
: 0.01,
P
value: 0.487). Unlike IgM, dIgA reactive to measles NP was not detected in most samples. The comparable diagnostic potential of anti-measles dIgA (area under the curve, AUC: 0.920–0.945) to anti-measles IgM (AUC: 0.986–0.995) suggests that dIgA may be a new blood-based marker of acute measles, independent of IgM, which merits further investigation and optimization.

IMPORTANCE
The world is facing a measles resurgence, and improved diagnostic tests for measles infection are an urgent World Health Organization research priority. Detection of measles-specific immunoglobulin M (IgM) as a standard diagnostic test has low positive predictive value in elimination settings, and there is a need for new biomarkers of measles infection to enable enhanced surveillance and response to outbreaks. We demonstrate the detection of measles-specific dimeric immunoglobulin A (dIgA) in patients with confirmed measles infections using a new indirect enzyme-linked immunosorbent assay protocol that selects for the dIgA fraction from total IgA in the blood. The magnitude of measles-specific dIgA responses showed a low correlation with IgM responses, and our results highlight the potential of dIgA for further development as an alternative and/or complementary biomarker to IgM for serological diagnosis of measles infection.