bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in Adherence to Dietary Guidelines for Hypertension: The Korea National Health and Nutrition Examination Surveys 1998–2012
Authors: Park Kyong , Cho SuKyung 
Published: November 2023

ObjectivesWe compared levels of adherence to the non‐pharmacological guidelines between patients with and without hypertension diagnoses, and examined changes in adherence during recent decades.MethodsWe used data from the Korean National Health and Nutrition Examination Survey (1998–2012), and 13,768 Korean hypertensive patients aged ≥30 years were categorized as with and without hypertension diagnoses based on blood pressure and self‐reported information. Adherence to the guidelines was calculated based on 6 components, including dietary and lifestyle habits. Multivariate generalized linear regression was used, accounting for a complex survey design.ResultsThe proportion of hypertensive patients aware of their condition increased from 33.4% in 1998 to 74.8% in 2012 (p for trend<0.001), although these increments plateaued during the recent survey years. Patients with hypertension diagnosis were older, female, and had lower education levels than those without hypertension diagnosis for most survey years. Overall adherence levels were poor (approximately 2 of 6 points), and levels of adherence to any of recommended non‐pharmacological habits were not significantly different between patients with and without hypertension diagnoses. However, overall adherence levels improved significantly among patients with hypertension diagnosis: from 2.09 in 1998 to 2.27 in 2012 (p=0.007), particularly regarding sufficient vegetable/seaweed consumption (p = 0.03), maintaining a normal weight (p = 0.03), and avoidance of smoking (p < 0.001).ConclusionsAwareness of hypertension is increasing, but Korean hypertensive patients have not been adequately educated in terms of disease management. Thus, there is a need for educational programs with continued implementation after patients are diagnosed with hypertensionSupport or Funding InformationThis research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Plannin(NRF‐2014R1A1A3049866).
Plasma Alkylresorcinol Concentrations as a Novel Measure of Compliance to a Gluten‐Free Diet
Authors: Lind Mads V , Madsen Mia L , Rumessen Jüri J , Vestergaard Henrik , Gøbel Rikke J , Hansen Torben , Lauritzen Lotte , Pedersen Oluf B , Kristensen Mette , Ross Alastair B 
Published: November 2023

BackgroundMany celiac disease (CD) patients experience difficulties in adherence to a gluten‐free diet. Methods for testing compliance to a gluten‐free diet are costly and cumbersome.ObjectiveA simple biomarker of gluten intake is needed in a clinical setting and will be useful for epidemiological studies investigating wider effects of gluten intake. The aim was to evaluate plasma total alkylresorcinols (AR) concentrations as a measure of gluten intake.DesignThis randomized, controlled, cross‐over intervention study in 52 Danish adults with features of the metabolic syndrome compared eight weeks gluten‐rich and gluten‐poor diet separated by a wash‐out period of at least six weeks. We measured fasting plasma concentrations of AR to determine if they reflected differences in gluten intake. Additionally, we investigated in 118 Danish adults the cross‐sectional association between self‐reported gluten intake and plasma AR in this and a similar study at baseline.ResultsTotal plasma AR decreased more during the gluten‐poor period compared to the gluten‐rich period −124.8 (−156.5; −93.0) vs. −31.8 (−63.1; −0.4) nmol/L, respectively, p<0.001. Based on the plasma AR‐profile we built a classification tree to objectively determine compliance and found an overall misclassification error of participants of 3.9%. The cross‐sectional study showed a 5.6% (2.4; 8.9) increase in total plasma AR per 1 g‐increase in reported gluten intake (p<0.001).ConclusionWe propose plasma AR as a new tool to monitor compliance to a gluten‐free diet to support clinical work with CD, as well as to help investigations on the possible effects of gluten in the wider population.Support or Funding InformationFunded by the Innovation Fund Denmark, grant no. 0603‐00487B (11‐116163). Some intervention products were sponsored by Kohberg, Lantmännen, AXA, Wasa, Urtekram, Finax, and Doves Farm. MVL is part sponsored by Cereal Partners Worldwide with an unrestricted grant. The study sponsors played no role in the design, methods, data‐management and analysis or in the decision to publish.
Dried Plum Consumption and Bone Mineral Density Retention in Postmenopausal Women: a Follow‐up Study
Authors: Arjmandi Bahram H. , Johnson Sarah A. , Pourafshar Shirin , Navaei Negin , Hooshmand Shirin , Chai Sheau C. 
Published: November 2023

Osteoporosis is a chronic condition characterized by low bone density and an increased risk of fracture. Although it affects both women and men, there is a much greater incidence in postmenopausal women. Certain lifestyle and nutritional factors are known to reduce the risk of osteoporosis and thereby the risk of fracture. In terms of nutrition, dried plum has been shown to be one of the most efficacious interventions in preventing and even reversing bone loss in postmenopausal women. In 2010, we finished a study in which we examined the effect of consuming 100 g dried plum versus a comparative control fruit (75 g dried apples) daily for one year in 160 postmenopausal women with osteopenia. Our published data indicated that women consuming dried plum had significantly higher bone mineral density (BMD) of the ulna and spine in comparison with those consuming dried apple. The purpose of the present study was to conduct a follow‐up evaluation on the extent to which individuals who received the dried plum intervention were able to retain BMD compared with those who received the dried apple intervention. A total of 20 participants were available for this follow‐up evaluation. BMD of the lumbar spine, forearm, hip, and whole body were assessed using DXA. Results showed that those who were in the dried plum group had significantly higher values of BMD of spine (P=0.012; %6) and ulna (P=0.002; %23) compared with those in the dried apple group. Importantly, participants in either group did not regularly consume dried plums as noted in their diet history. Our findings suggest that women in the dried plum group retained their BMD to a greater extent than those in the dried apple group, even after a 5‐year period in the absence of regular dried plum consumption. Our observations suggest a long‐lasting bone‐protective effect of dried plum; however, other dietary, lifestyle, and pharmaceutical factors need to be evaluated.Support or Funding InformationCalifornia Dried Plum Board
Comparing the Effects of Dairy Saturated Fatty Acids from Butter and Cheese on Non‐Lipid Cardiovascular Risk Factors in Men and Women
Authors: Brassard Didier , Tessier‐Grenier Maude , Rajendiran Ethendhar , She Yongbo , Ramprasath Vanu , Gigleux Iris , Levy Emile , Tremblay Angelo , Jones Peter , Couture Patrick , Lamarche Benoit 
Published: November 2023

OBJECTIVEThe primary objective of this study was to compare the impact of dairy saturated fatty acids (SFA) provided as cheese (CHEESE) or butter (BUTTER) on blood pressure, fasting glucose and C‐reactive protein (CRP) levels. The impacts of SFA from CHEESE were also compared to low SFA diet with either high monounsaturated (MUFA), polyunsaturated fatty acids (PUFA) and carbohydrate (CHO) content.METHODSThese are preliminary data from 57 men and women, with abdominal obesity and with relatively low HDL‐C concentrations, enrolled in an on‐going multicenter randomized crossover controlled trial consisting of 5 isoenergetic diets of 4 weeks each (table 1): 1) a diet rich in SFA from cheese (CHEESE); 2) a diet rich in SFA from butter (BUTTER); 3) a diet rich in mono‐unsaturated fatty acids (MUFA); 4) a diet rich in poly‐unsaturated fatty acids (PUFA); and 5) a low‐fat, high carbohydrate diet (CHO). All foods were provided to participants in the context of this full feeding study. Fasting blood samples, office systolic (SBP), diastolic blood pressure (DBP) and anthropometric measurements were taken at the end of each intervention.RESULTSMean age (±SD) of participants was 40.6 ± 13.6 years. Mean waist circumference was 107.7±11.3 cm in men and 94.9 ± 13.7 cm in women. No difference in body weight was observed among treatments. CHEESE compared with BUTTER had no significant impact on SBP (p=NS), DBP (p=NS), CRP (p=NS) and fasting glucose (p=NS) levels. CHEESE compared with the PUFA, MUFA and CHO diets also had no impact on these risk factors. There was also no statistically significant effect of diets on SBP (p=NS), DBP (p=NS), CRP (p=NS) or fasting glucose (p=NS) levels.CONCLUSIONSAs part of an overall healthy diet, these results suggest no deleterious short‐term effects of SFA from dairy, irrespective of the source (butter or cheese), on non‐lipid cardiovascular risk factors in men and women with abdominal obesity and with relatively low HDL‐C levels.Support or Funding InformationDairy Research Cluster Initiative (Dairy Farmers of Canada, Agriculture and Agri‐Food Canada, the Canadian Dairy Network and the Canadian Dairy Commission)
Macronutrient (% energy), calcium and sodium content of experimental diets

CHEESE
BUTTER
MUFA
PUFA
CHO

Lipids (%)
32
32
32
32
25

SFA (%)
13
13
6
6
6

MUFA (%)
14
14
21
14
14

PUFA (%)
5
5
5
12
5

Carbohydrates (%)
52
52
52
52
59

Proteins (%)
16
16
16
16
16

Calcium (mg/2500 kcal)
1261
812
812
812
842

Sodium (mg/2500 kcal)
2482
2480
2479
2479
2485
Long‐Term Effects of Early‐Life Exposure to Olanzapine
Authors: George Brianna , Ramalingam Latha , Menikdewella Kalhara , Soto Paul , Moustaid‐Moussa Naima 
Published: November 2023

An increasing number of children and young adults are prescribed antipsychotic medications, such as olanzapine, which is known to cause excessive weight gain and other metabolic issues. This has raised concerns of long‐term complications such as obesity, diabetes and heart disease. We propose to use fish oil, with known triglyceride lowering effects and potential anti‐obesity properties, to counteract obesity and other metabolic complications associated with antipsychotic drugs. Hence, we designed this project to test the effects of olanzapine and fish oil, individually or in combination in mice fed high fat diets. Specifically, male C57BL/6J mice (N=32) were given plain cookie dough or cookie dough with olanzapine (3 and then 6 mg/kg/day) from postnatal day (PND) 35 to 63. Mice were maintained on a high fat (HF)‐high sucrose (HS) diet, with or without fish oil (FO) supplementation, resulting in four groups of mice (n=8 per group): HF‐HS, HF‐HS with olanzapine, HF‐HS‐FO, and HF‐HS‐FO with olanzapine. Body weight and food consumption were measured daily. Glucose and insulin tolerance were assessed in adulthood (≥ PND 90). Mice exposed to FO and olanzapine had lower body weights compared to HF‐HS diet alone, despite equivalent amounts of food consumed between the groups. Glucose clearance rates were higher in the HF‐HS with olanzapine and FO supplementation group compared to HF‐HS fed mice. In conclusion, the results demonstrated long‐lasting effects of early‐life exposure to olanzapine on body weight and metabolism that were comparable to fish oil effects. Gene expression analyses of genes related to lipid metabolism, obesity, and diabetes, as well as triglyceride content in the liver are currently underway to determine the mechanisms mediating long‐term effects of olanzapine on body weight and liver metabolism.Support or Funding InformationTexas Tech University Presidential Cluster Hire Tier 2 and Obesity Research Cluster Pilot and Feasibility Award
lncRNA with DNA Motive Putative Epigenetic Regulators in Atherosclerosis
Authors: Flores Fabiola Estefania Tristan , Martinez Guillermo Antonio Silva , Ríos Dalia Rodríguez , Caudillo Yolanda Alvarado , Villate Plinio Guzmán , Lund Gertrud , Zaina Silvio 
Published: November 2023

Atherosclerosis is characterized by lipid infiltration into the arterial wall accompanied with inflammation. In previous studies, a DNA motif called differential methylation motive (DMM) which is involved aberrant methylation changes was identified in atherosclerotic aortas. We pretend to identify the role of the DMM in atherosclerosis. Hypomethylation in advanced lesions compared with middle lesions in long noncoding RNA (lncRNA) with DMM in, also identified one binding protein to DMM is LXRβ involved in reducing atherosclerotic plate. We demonstrated that LXRβ is binding to intergenic lncRNA that containing the DMM, further stimulus with the agonist of LXRβ regulates the expression of lncRNA adjacent to target genes of LXRβ. Also, we identified an inverse expression patterns in atherosclerotic aorta between lncRNA adjacent gene, suggesting the regulation in cis on genes.Support or Funding InformationCONACyT
Morphological Characterization of Triple Negative Breast Cancer Cells
Authors: Jogalekar Manasi P , Serrano Elba E 
Published: November 2023

Triple negative breast cancer (TNBC) is characterized by the lack of receptors for estrogen (ER), progesterone (PR) and human epidermal growth factor (HER2). Reports suggest that cancer cells maintained in three‐dimensional (3D) cultures are biologically more similar than monolayer cultures to in vivo tumor tissue. We undertook the current study to enable design of a 3D platform that may afford success in developing interventions to treat TNBC. To this end, we determined the morphological impact of growing a triple negative breast cancer cell line, HCC70 in monolayer culture on tissue culture polystyrene, as compared with growth in 3D culture using extracellular matrix Geltrex™. When we analyzed the morphology of cells in culture at the resolution of light microscopy using phase contrast and laser confocal imaging methods, we found that monolayer cells appeared flat, adhered to the polystyrene surface, and formed clusters with neighboring cells. In contrast, matrix‐organized cells formed compact spheroids. Ultra‐structural characterization was achieved using quantitative analysis of images captured with transmission electron microscopy. Our results showed that the 3D cultures had a higher number of mitochondria, autophagic vacuoles and intercellular junctions than those present in monolayer cultures. In addition, 3D cultures had membrane protrusions which were not present in monolayer cultures; we hypothesize that these structures may play an important role in matrix invasion. Our results demonstrate that the morphology of triple negative breast cancer cells is influenced by the surrounding environment. Our results suggest that 3D culture systems may provide a useful alternative platform for testing anti‐cancer treatments in triple negative breast cancer cell lines as compared to monolayer culture systems.Support or Funding InformationNIH grant P50GM068762 and the New Mexico State University's (NMSU) Manasse Endowment to Dr. Elba Serrano.
Effect of probiotics and Atractylodes/Taraxacum against DSS‐induced colitis in mouse
Authors: Park Min young , Kwon Oran 
Published: November 2023

By constituting barrier surfaces, intestinal epithelial cell plays a critical role on maintenance of gut health. Dysregulated immune responses and destruction of cell in intestine causes breakdown in intestinal balance. Dextran sulphate sodium (DSS) is the most widely used chemical for induction of colitis in animal. Thus its treatment induces colonic inflammation, acute diarrhea, and shortening of intestine length with clinical and histological similarity to human ulcerative colitis (UC). Recent researches have shown that probiotics could support gut health by providing good gut microbes and playing beneficial roles in gut. And Atractylodes/Taraxacum was used as oriental medicine for intestinal function. So we hypothesized that probiotics lactobacillus gasseri BNR17 (BNR17) and Atractylodes/Taraxacum could help enhancing gut health through reducing destruction of colon tissue and pro‐inflammatory cytokine increased in DSS treated mouse. Female Balb/c mice (7 weeks old, n=20) were acclimated for 1 week and randomized into 4 groups; normal control group (NC), DSS treated group (positive control, DSS), DSS + BNR17 (DP) and DSS + Atractylodes/Taraxacum treatment at 100 mg/kg B.W. (DA) groups. Materials were treated by gavage for 14 days. 3% of DSS in drinking water was provided and Disease activity index (DAI) was estimated during the last 7 days. Mice were fed chow diet ad libitum. At the end of experimental period, colon length and weight were measured followed by H&E staining to determine the level of mucosal inflammation, cell destruction, and mucin secretion. Body weight, DAI and colon length of DSS group were significantly different with NC group. Colon length was also decreased in DSS induced group. In material administration groups, body weight and DAI tended to increase in DA compared to DSS group. Inflammation and cell destruction of colon tissue observed in histopathology were reduced in DP and DA. Mucin secretion was increased in DA group. These results suggest that probiotics and Atractylodes/Taraxacum has capability of alleviating DSS‐induced colitis by reducing damage and inflammation of colon tissue in mouse. We will examine immunohistochemistry and measure target gene expression relate with immune response, cell proliferation/destruction, and mucin secretion with matching morphological data in further study.Support or Funding InformationThis work was supported by the Ministry of Science, ICT & Future Planning (Bio‐synergy Research Project NRF2012M3A9C4048761) and BK21 PLUS through the National Research Foundation.
Flavonoid Intake and Mortality from Cardiovascular Disease and All Causes: A meta‐analysis of prospective cohort studies
Authors: Kim Youngyo , Je Youjin 
Published: November 2023

BackgroundAccumulating studies have suggested that flavonoid intake is associated with a decreased risk of coronary heart disease and cardiovascular disease (CVD). There are many epidemiological studies on flavonoid intake and mortality, but no comprehensive investigation has yet been conducted.ObjectiveTo quantitatively assess the association between flavonoid intake and mortality from CVD and all‐causes, we performed a meta‐analysis of prospective cohort studies.DesignEligible studies were identified by searching PubMed and Web of Science databases for all articles published up to June 2015 and via hand searching. Study‐specific estimates adjusting for potential confounders were combined to calculate a pooled relative risk (RR) with 95% confidence interval (CI) using a random‐effects model.ResultsA total of 15 publications that examined the association between flavonoid intake and mortality from CVD and all‐causes were identified. The pooled RR of CVD mortality for the highest versus lowest category of flavonoid intake was 0.85 (95% CI: 0.74, 0.98). By subclass of flavonoids, all classes showed significant inverse associations, except flavonols and isoflavones. For total mortality, high flavonoid intake is weakly associated with lower total mortality (pooled RR = 0.78, 95% CI: 0.72, 1.01). The associations did not differ substantially by sex and geographical region.ConclusionsOur findings indicate that higher intake of flavonoids is associated with a reduced risk of mortality from CVD and all causes in men and women. These results support current recommendations of high fruit and vegetables intake as a part of a healthy diet.Support or Funding InformationThis work was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (NRF‐2015R1A1A1A05001362).
Effect on One Time Application of Functional Cerebrospinal Therapy (FCST): A Retrospective Clinical Survey
Authors: Jung Jeeyoun , Lee Somin , Jerng Ui Min 
Published: November 2023

ObjectivesThe aim of this study was main symptoms of outpatient in Lee young Jun clinics who developed the functional cerebrospinal therapy and to investigate the therapeutic effects on one time application of functional cerebrospinal therapy (FCST).MethodsPatients who visited at least twice at an oriental medical clinic and treated with FCST were included. They were asked to write 216 numeric rating scale(NRS)‐based questionnaire about degree of various symptoms at each visit. Prevalence and degree decrease of all reported symptoms were analyzed.ResultsTotal of 744 outpatients were included. There were fourteen symptoms of which more than half of all patients complained. All symptoms were significantly reduced after one administration of FCST. About four to seven percent of patients reported at least five‐point reduction on NRS in each symptom after FCST.ConclusionFCST might have potential immediate effect not only TMJ related symptoms, but also all sorts of pain, psychological symptoms, and fatigue‐related symptoms.Support or Funding InformationThis work was supported by grants from Korea Institute of Oriental Medicien(C15110)
Resveratrol Increases Catecholamine Synthesis in Macrophages: Implications on Obesity
Authors: Parmar Ashish , Mula Ramanjaneya V.R , Azhar Yusra , Shashidharamurthy Rangaiah , Rayalam Srujana 
Published: November 2023

Sympathetic activation of white adipocytes results in the transdifferentiation of white adipocytes into brown like thermogenic adipocytes called beige adipocytes. White adipose tissue (WAT) is composed of several cell types including macrophages and activation of anti‐inflammatory M2 macrophages was recently shown to induce beiging of WAT in rodent models. M2 macrophages secrete catecholamines which play a predominant role in the induction of beiging in WAT. In the current study, we demonstrate novel anti‐obesity effects of resveratrol (RES), a phytoalexin, mediated through catecholamine synthesis in RAW 264.7 macrophage cell line. RES significantly increased the mRNA expression of tyrosine hydroxylase, phenylalanine N‐methyl transferase, dopamine beta hydroxylase and phenyl alanine hydroxylase expression in these cells. In addition, RES increased arginase, a marker for M2 phenotype and decreased LPS‐induced iNOS (inducible nitric oxide synthase), a marker for M1 phenotype. Furthermore RES decreased nitric oxide production from LPS‐treated RAW264.7 cells. To demonstrate the effect of RES on catecholamine secretion, we examined the cell culture supernatant from RES treated RAW264.7 cells for catecholamine levels. The results indicate that RES significantly increased catecholamine production in LPS‐treated RAW264.7 cells. Finally, upregulation of thermogenic markers was seen in mature 3T3‐L1 adipocytes incubated with conditioned media from RAW264.7 cells treated with RES. These studies suggest a novel anti‐obesity mechanism for RES by increasing catecholamine production in macrophages.Support or Funding InformationThis work was supported by the Biomedical sciences graduate research program at Philadelphia College of Osteopathic Medicine – GA Campus.