bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel prognostic autoantibodies in diffuse large B‐cell lymphoma treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone via a high‐throughput antigen microarray
Authors: Dai Liyuan , Chen Haizhu , Tan Qiaoyun , Wang Yanrong , Li Lin , Lou Ning , Fan Guangyu , Xie Tongji , Luo Rongrong , Wang Shasha , Zhou Yu , Zhong Qiaofeng , Yao Jiarui , Zhang Zhishang , Tang Le , Shi Yuankai , Han Xiaohong 
Published: December 2023

AbstractBackgroundR‐CHOP (rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone) is a standard first‐line treatment for diffuse large B‐cell lymphoma (DLBCL). However, 20%–40% of patients survive less than 5 years. Novel prognostic biomarkers remain in demand.MethodsBaseline plasma autoantibodies (AAbs) were assessed in 336 DLBCLs. In the discovery phase (n = 20), a high‐density antigen microarray (∼21,000 proteins) was used to expound AAb profiles. In the verification phase (n = 181), with a DLBCL‐focused microarray, comparative results based on event‐free survival at 24 months (EFS24) and lasso Cox regression models of progression‐free survival (PFS) and overall survival (OS) were integrated to identify potential biomarkers. They were further validated by enzyme‐linked immunosorbent assay in validation phase 1 (n = 135) and a dynamic cohort (n = 12). In validation phase 2, a two‐AAb‐based risk score was established. They were further validated in an immunohistochemistry cohort (n = 55) and four independent Gene Expression Omnibus datasets (n = 1598).ResultsFour AAbs (CREB1, N4BP1, UBAP2, and DEAF1) were identified that showed associations with EFS24 status (p < .05) and superior PFS and OS (p < .05). A novel risk score model based on CREB1 and N4BP1 AAbs was developed to predict PFS with areas under the curve of 0.72, 0.71, 0.76, and 0.82 at 1, 3, 5, and 7 years, respectively, in DLBCL treated with R‐CHOP independent of the International Prognostic Index (IPI) and provided significant additional recurrence risk discrimination (p < .05) for the IPI. CREB1 and N4BP1 proteins and messenger RNAs were also associated with better PFS and OS (p < .05).ConclusionsThis study identified a novel prognostic panel of CREB1, N4BP1, DEAF1, and UBAP2 AAbs that is independent of the IPI in DLBCL.
It takes a village to grow a tree: Most tree species benefit from dissimilar neighbors
Authors: Di Maurizio Vanessa , Searle Eric , Paquette Alain 
Published: December 2023

AbstractScientific consensus is that diverse tree species positively impact forest productivity, especially when species are functionally dissimilar. Under the complementarity hypothesis, differences in species traits reduce competition among neighboring tree species. However, while this relationship has been extensively studied at the community level, there is a lack of understanding regarding how individuals of different species specifically respond to a functionally dissimilar neighborhood. In this study, we used permanent plots from Quebec, Canada, and 19 focal tree species to test whether: (1) tree growth response to neighborhood dissimilarity varies with their identity and competition intensity, and (2) focal tree species’ traits explain their response to neighborhood dissimilarity. We demonstrate that: tree growth is primarily influenced by competition, species identity, and their interactions, but that dissimilarity, alone and in interaction with the main drivers of tree growth, explains an additional 1.8% of the variation in species growth. Within this context, (1) most species’ respond positively to neighborhood dissimilarity, with magnitude being species and competition dependent, and (2) focal tree traits partly explain these dependencies, with shade‐intolerant species benefiting most from dissimilar neighbors under high competition. Our study provides empirical support for the complementarity hypothesis, emphasizing the small but consistent positive effect of functional dissimilarity on tree growth in local neighborhoods. Our findings identify the species with the highest potential of benefiting from dissimilar neighbors but also demonstrate that the positive effect of neighborhood dissimilarity is not limited to a select few species with specific traits; rather, it is observed across a diverse range of species. The cumulative growth responses of individuals to functionally dissimilar neighbors may help explain the commonly observed higher productivity in more diverse communities.
Integrated phylogenetic analyses reveal the evolutionary, biogeographic, and diversification history of Asian warty treefrog genus Theloderma (Anura, Rhacophoridae)
Authors: Luo Tao , Zhao Xin‐Rui , Lan Chang‐Ting , Li Wei , Deng Huai‐Qing , Xiao Ning , Zhou Jiang 
Published: December 2023

AbstractAsian warty treefrogs, genus Theloderma, are morphologically variable arboreal frogs endemic to Southeast Asia and Southern China. However, integrated systematic studies are lacking, and knowledge of the genus in terms of diversity, origin, and historical diversification remains limited. To address these knowledge gaps, we used three mitochondrial and five nuclear gene fragments to reconstruct the Theloderma phylogeny, estimate divergence times, and examine the biogeography of the genus. Phylogenetic and species delimitation analyses suggest that the genus Theloderma comprises three major clades corresponding to two subgenera and seven species groups, and mPTP identified at least 12 putative cryptic species, suggesting that species diversity has been underestimated. Biogeographic analyses indicated that most recent common ancestor of Theloderma originated in the Indochina Peninsula during the Middle Oligocene (ca. 27.77 Ma) and the splitting of Clade A to C occurred in the Late Oligocene (ca. 23.55–25.57 Ma). Current biogeographic patterns result from two distinct processes: in situ diversification in the Indochina Peninsula and dispersal in multiple areas, namely southward dispersal to the Malay Peninsula and Borneo, northeastward dispersal to Southern China, northward dispersal to the Himalayas, and dispersal from Southern China to the Indochina Peninsula. Ancestral character reconstruction suggests that the ancestor of Theloderma may have possessed a small body size, rough dorsal skin, and absence of vomerine teeth and hand webbing, and that these four characters have undergone multiple evolutions. Principal component analysis based on eight bioclimatic variables did not clearly distinguish the three major clades of Theloderma, suggesting that species in these clades may occupy similar climatic ecological niches. Our research highlights the importance of orogeny and paleoclimatic changes, in shaping amphibian biodiversity in mountain ecosystems.
Prospects of pollinator community surveillance using terrestrial environmental DNA metagenetics
Authors: Avalos Grace , Trott Regina , Ballas John , Lin Chia Hua , Raines Clayton , Iwanowicz Deborah , Goodell Karen , Richardson Rodney T 
Published: December 2023

AbstractCurrent pollinator survey methods exhibit bias, require highly‐trained practitioners, and are difficult to scale to large sample sizes. High‐throughput sequencing of terrestrial eDNA could provide a complementary tool for studying pollinator communities, but eDNA methods have not been extensively evaluated. We conducted metagenetic analysis of whole arthropod community eDNA from 20 flower and seven honey bee‐collected pollen samples and compared eDNA‐derived data with traditional netting‐based surveys of the pollinator communities present during sampling. We focused our analysis on Anthophila (bees) and detected eight bee genera belonging to four families across COI, 16S, and 28S markers. Results varied considerably by marker and eDNA substrate. Detected bee genera were plausible for the study system and about 43 percent of total bee genera were detected with both eDNA and net‐based surveys, though netting resulted in more detections across a wider diversity of genera. Data from sequenced controls suggest that eDNA identifications were unlikely to have resulted from cross‐contamination. Our results demonstrate that bee communities can be documented with eDNA techniques and that the choice of marker and substrate substantially influences detection. Future improvements to our methods are required, but eDNA surveys appear well‐suited to characterize diverse pollinator communities and provide novel sampling perspectives within plant‐pollinator networks. Future efforts should focus on improving the selection of markers available for pollinator eDNA metagenetics, addressing taxonomic gaps within reference sequence databases and optimizing sampling and eDNA isolation protocols. We anticipate that such improvements are highly feasible and that eDNA will be a useful tool to those who study pollinators and plant‐pollinator interactions.
Three different oils improve HFD‐induced lipid metabolism disorders via driving gut microbiota
Authors: Lai Meiying , Liu Yilin , Han Xianjing , Chen Yihan , Liu Bin , Zeng Feng 
Published: December 2023

AbstractSchizochytrium sp. L. oil (SO), fish oil (FO), and sacha inchi oil (SIO) can regulate high‐fat diet‐induced obesity, but the effect on gut microbiota and the mechanism remain unclear. This study aimed to find out the different effects of the three oils mediated the gut microbiota on lipid metabolism. In comparison with the high‐fat diet (HFD) group, SO, FO, and SIO could reduce fat mass accumulation and decrease the levels of the serum and liver biochemical parameters in mice. The regulatory effect on gut microbiota in mice is determined by 16S rRNA gene high‐throughput sequencing. Caulobacter, Acetatifactor, and Mucispirillum as dominant bacterial genus are enriched in intervention groups. SO, SIO, and FO could distinctly decrease interleukin 6, nuclear factor‐kappa B, tumor necrosis factor‐alpha, sterol regulatory element‐binding protein 1c, FAS, and HMGCR expressions in liver, upregulate the expression of acyl‐coenzyme A oxidase 1 and A acetyltransferases and regulate the thermogenesis. SO, SIO, and FO has a comprehensive impact on lipid metabolism by the expression of genes related to fat synthesis, cholesterol synthesis, inflammation, energy consumption, the structure of intestinal flora, abundance of beneficial bacteria, and intestinal diversity. Based on these results, SO, SIO, and FO may be used as functional food to drive beneficial changes in gut microbiota and then regulate the lipid metabolism disorders.Practical Applications: Obesity‐related diseases caused by lipid metabolism disorders have driven the rapid development of the diet drug market. However, the side effects of diet drugs on the human body also make the majority of consumers worried. Natural oil products that regulate lipid metabolism disorders and dietary obesity provide a substitute. Schizochytrium sp. L. oil (SO), fish oil (FO), and sacha inchi oil (SIO) are rich in high‐quality unsaturated fatty acids and are not suitable for high temperature cooking but can be eaten in low temperature cooking and sauces and so on. SO, FO, and SIO can be used to develop functional food that could reduce lipid metabolism disorders.
Stabilization of infant formulas against lipid oxidation: What are the key structural levers?
Authors: Cancalon Mathilde , Barouh Nathalie , Hemery Youna , Baréa Bruno , Durand Erwann , Bourlieu‐Lacanal Claire , Villeneuve Pierre 
Published: December 2023

AbstractInfant follow‐on formulas (IFF) mimic human milk. This study aimed to identify the key structural levers that influence oxidative stability of IFF. Representative model IFF of marketed products in term of lipid composition were formulated with varying droplet size, lipid droplet core composition, and interfacial composition using different emulsifiers (soy lecithin or dairy phospholipids [DPL]). The oxidative stability of model IFF was assessed in accelerated storage conditions. No significant stabilization effect based on the lipid droplet core composition was observed. However, the nature of the interface, influenced by the emulsifier type, had an impact. Model IFF with DPL showed no loss of tocopherols and peroxide value was up to twice lower than those with soy lecithin after 20 days. This effect was particularly pronounced for the 0.4µm droplets. These results suggest that model IFF with DPL had a greater oxidative stability, likely due to the presence of sphingomyelin and the formation of a rigidified domain at the droplet surface. Model IFF with 0.4 µm droplets stabilized with soy lecithin, especially when added to the water phase, showed a tocopherols loss twice as high as that of IFF with DPL. These results indicate that oxidative stability of IFF is more influenced by the chemical environment rather than droplet size.Practical Application: Infant follow‐on formulas (IFF) aim to ensure an adequate nutritional intake to support the proper development and infant growth. Therefore, IFF must be stable against degradation phenomena such as lipid oxidation and have a composition and structure that are as biomimetic as possible to mature breast milk. This study provides key information for the development of IFF with a lipid composition and structure that are suitable for the infant nutritional needs and have an acceptable resistance to lipid oxidation. More generally, these results can be applied to all dispersed systems in the form of oil‐in‐water emulsions with a similar composition.
Intracerebral electrographic activity following a single dose of diazepam nasal spray: A pilot study
Authors: Privitera Michael D. , Mendoza Lucy C. , Carrazana Enrique , Rabinowicz Adrian L. 
Published: December 2023

AbstractObjectiveRescue benzodiazepine medication can be used to treat seizure clusters, which are intermittent, stereotypic episodes of frequent seizure activity that are distinct from a patient's usual seizure pattern. The NeuroPace RNS® System is a device that detects abnormal electrographic activity through intracranial electrodes and administers electrical stimulation to control seizures. Reductions in electrographic activity over days to weeks have been associated with the longer‐term efficacy of daily antiseizure medications (ASMs). In this pilot study, electrographic activity over hours to days was examined to assess the impact of a single dose of a proven rescue therapy (diazepam nasal spray) with a rapid onset of action.MethodsAdult volunteers (>18 years old) with clinically indicated RNS (stable settings and ASM usage) received a weight‐based dose of diazepam nasal spray in the absence of a clinical seizure. Descriptive statistics for a number of detections and a sum of durations of detections at 10‐min, hourly, and 24‐h intervals during the 7‐day (predose) baseline period were calculated. Post‐dose detections at each time interval were compared with the respective baseline‐detection intervals using a 1 SD threshold. The number of long episodes that occurred after dosing also were compared with the baseline.ResultsFive participants were enrolled, and four completed the study; the excluded participant had recurrent seizures during the study. There were no consistent changes (difference >1 SD) in detections between post‐dose and mean baseline values. Although variability was high (1 SD was often near or exceeded the mean), three participants showed possible trends for reductions in one or more electrographic variables following treatment.SignificanceRNS‐assessed electrographic detections and durations were not shown to be sensitive measures of short‐term effects associated with a single dose of rescue medication in this small group of participants. The variability of detections may have masked a measurable drug effect.Plain Language SummaryRescue drugs are used to treat seizure clusters. Responsive neurostimulation (RNS) devices detect and record epilepsy brain waves and then send a pulse to help stop seizures. This pilot study looked at whether one dose of a rescue treatment changes brain activity detected by RNS. There was a very wide range of detections, which made it difficult to see if or how the drug changed brain activity. New studies should look at other types of brain activity, multiple doses, and larger patient groups.
Long‐term drinking of green tea combined with exercise improves hepatic steatosis and obesity in male mice induced by high‐fat diet
Authors: Wang Ruru , Gu Mingxing , Zhang Yanzhong , Zhong Qinglin , Chen Linbo , Li Daxiang , Xie Zhongwen 
Published: December 2023

AbstractDietary habits and exercise play an important role in the well‐being of human health. Currently, how long of drinking tea combined with exercise could efficiently ameliorate hepatic steatosis and obesity still needs to be investigated. Here, short‐term and long‐term green tea drinking combined with exercise were studied to improve hepatic steatosis and obesity in high‐fat diet‐induced (HF) mice. Our results showed that Yunkang 10 green tea (GT) combined with exercise (Ex) exhibited synergistic prevention effects on ameliorating hepatic steatosis and obesity. Especially, 22‐week intervention with GT or Ex improved all symptoms of obesity, which indicated that long‐term intervention exhibited profound preventive effects than the short term. Moreover, the combined intervention of 22 weeks inhibited the activation of NF‐κB pathway and the expression of proinflammatory cytokines, which suggests that tea combined exercise may improve liver steatosis mainly by inhibiting inflammation. The key molecules for regulating lipid and glucose metabolism SCD1 were obviously downregulated, and GLU2 and PPARγ were significantly upregulated by GT and exercise in the liver of high‐fat diet‐induced mice. This study demonstrated that long‐term intervention with GT and exercise effectively relieved hepatic steatosis and obesity complications by ameliorating hepatic inflammation, reducing lipid synthesis, and accelerating glucose transport.
Relative preference for distinct reinforcers maintaining destructive behavior
Authors: Norris Halle M. , Greer Brian D. 
Published: December 2023

AbstractThe literature offers few recommendations for sequencing exposure to treatment conditions with individuals with multiply maintained destructive behavior. Identifying relative preference for the functional reinforcers maintaining destructive behavior may be one means of guiding that decision. The present study presents a preliminary attempt at developing a robust relative preference and reinforcer assessment for individuals with multiply maintained destructive behavior. Guided and free‐choice trials were implemented in which participants chose between two multiple‐schedule arrangements, each of which programmed signaled periods of isolated reinforcer availability and unavailability. Consistent participant choice and responding during free‐choice trials was then used to thin the corresponding schedule of reinforcement. The results demonstrated a strong preference for one of the two functional reinforcers for all four participants, yet preferences differed across participants and were not well predicted by responding in prior analyses.
A multi‐centre survey of New Zealand cancer patients' preferences for radiation treatment information
Authors: Flockton Alannah , Leong Aidan , Gilfillan Daniel , Larsen Peter 
Published: December 2023

AbstractIntroductionPatients undergoing radiation therapy have a diverse range of information needs, however, there is a lack of data specific to the needs of New Zealand patients. This cross‐sectional survey captured New Zealand cancer patients' preferences for radiation treatment information. Preferences were assessed regarding the scope of information needs and the satisfaction with which these needs were being met.MethodsA custom survey was offered to 275 eligible patients undergoing radiation treatment at six of 10 departments across New Zealand over a 2‐day period. The survey captured patient demographics as well as information needs and satisfaction across five distinct domains using Likert scales and one free‐text question. Responses were analysed using descriptive statistics and directed content analysis.ResultsOver 80% of participants rated all information domains as extremely or very important. Over 90% of participants were extremely satisfied or very well satisfied in four of the five domains. Information on what happens after radiation treatment had the lowest satisfaction at 78%. No demographic subcategories were clearly associated with differences in information needs or satisfaction. 59% of participants indicated their willingness to engage in online education. The qualitative analysis strongly corroborated the quantitative results.ConclusionThe New Zealand cancer patients surveyed in this study demonstrated high levels of importance and satisfaction with the information provided during radiation treatment. Information on what happens after completing radiation treatment had the lowest satisfaction. These findings support further exploration of New Zealand cancer patients' information needs following radiation treatment as well as strategies to address them.
Molecular epidemiology and genetic diversity of norovirus among hospitalized patients with acute gastroenteritis in Shandong, China, 2016–2018
Authors: Li Chao , Song Shaoxia , Huang Xianglin , Liu Xiaolin , Lv Hui , Shen Yuanyuan , Wei Xuemin , Zhang Wenqiang , Xu Yifei 
Published: December 2023

AbstractNorovirus (NoV) infection is a leading cause of acute gastroenteritis (AGE) for people of all ages. Here, we reported the molecular epidemiology and genetic diversity of NoVs among hospitalized patients with AGE between 2016 and 2018 in Shandong Province, China. Two thousand sixty‐nine AGE patients from sentinel hospitals were enrolled. The stool samples were collected and tested for NoVs by real‐time RT‐PCR. The RNA‐dependent RNA polymerase (RdRp) and capsid gene of 163 strains were amplified and sequenced for genotyping. Phylogenetic analyses and genomic characterization were conducted with the VP1 and RdRp region of the full genome sequences. Four hundred seventy two (21.76%) samples were NoV‐positive. The positive rate in 2016 was higher than those of 2017 and 2018. We observed diverse NoV genotypes. GII.2[P16] emerged in January 2017 and became the dominant genotype between May and June 2017. Phylogenetic analyses showed that our GII.2[P16] genomes clustered in the SC1 in VP1 region, while they belonged to the Emerging GⅡ.P16 (2015−2017) clade in RdRp region. Our GⅡ.4 strains displayed two amino acid mutations, positions R297H and D372N, in epitope A of the VP1 region. Our study highlighted that NoV is an important pathogen of viral AGE in Shandong and, therefore, it is necessary to strengthen its surveillance.
New Strategies for Remnant Ear Treatment in Microtia Reconstruction Based on Morphometric Studies
Authors: Xu Zhicheng , Li Yiyuan , Li Datao , Zhang Ruhong , Zhang Qun , Xu Feng , Chen Xia 
Published: December 2023

ObjectiveGiven the lack of specific evaluation indices, it is difficult to determine whether to transpose or abandon remnant ears in lobule‐type microtia reconstruction. The authors illuminate referable parameters beneficial for proper treatment of remnant ear in an efficient manner.MethodsA series of 359 lobule‐type microtia patients underwent autogenous costal cartilage auricular reconstruction between 2016 and 2021. Fourteen measuring points and defined distances as well as six ratios of specific distances based on position, plumpness, similarity and the width‐to‐length ratio of the remnant ear have been described, and relevant tactics for appropriate treatments are introduced.ResultsDefinite morphometric results contribute to attaining satisfactory contours of reconstructed auricles with harmonious earlobes, which exhibit highly similar dimensions and appearances compared to the contralateral normal ears.ConclusionWith the help of the proposed locating points and measuring approaches, the procedure of remnant ear treatment is systematically clarified. This technique ensures operation safety and contributes to the aesthetic contour of the auricle.Level of EvidenceIV Laryngoscope, 134:2741–2747, 2024