bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Surface Treatments on the Shear Bond Strength of Composite Resin and RMGIC to Biodentine: An In-Vitro Sem Study
Authors: Thomas Minu , Hari Krishnan , Mathew Joy , Gaffoor Faisal M. A. , Yumnam Gargi , Rajendran Rakhi 
Published: January 2025

ABSTRACT

Background and Objectives:
This study aimed to evaluate and contrast the impact of various surface treatments on the shear bond strength (SBS) of composite resin and RMGIC on Biodentine.

Materials and Methods:
Sixty acrylic blocks with 4mm diameter and 2mm depth central cavities were made, and Biodentine (Septodont, France) was filled into the cavities and sorted into three groups (n = 20) based on the different surface treatments on Biodentine. The SBS was evaluated by employing a knife-edge blade in a universal testing machine, and fractured surfaces were analyzed under a Scanning electron microscope.

Results:
The study results indicated that the use of 9% hydrofluoric acid etching with Biodentine resulted in significantly greater SBS compared to the other groups, namely 37% phosphoric acid etching with Biodentine and intraoral sandblasting with Biodentine.

Conclusion:
9% hydrofluoric acid etching was the preferred choice of surface treatment on Biodentine in vital pulp therapy and composite resin showed better SBS with Biodentine.
Comparative Micro-CT Evaluation of Dentinal Tubule Penetration of BIO-C and AH Plus Jet Root Canal Sealer Using Three Intra-Canal Activation Techniques
Authors: Vivek K. , Sreedhar Sreeja , Nair Siddharth V. , Sreelekshmi V. , Jitha J. , Viswanadhan Anitha , Athira P. M 
Published: January 2025

ABSTRACT

Objectives:
This study aimed to compare the effectiveness of three intra-canal sealer activation techniques: ultrasonic, Lentulospiral activation, and GP cone-mediated ultrasonic activation on dentinal tubule penetration of a bioceramic (BIO-C) root canal sealer and AH Plus jet sealer with single cone obturation.

Materials and Methods:
Eighty single-rooted straight canal teeth were randomly divided into two main groups – BIO-C sealer and AH Plus jet sealer (n = 40). Each group was further divided into four subgroups, based on the three activation techniques: ultrasonics, Lentulospiral, gutta-percha cone-mediated ultrasonic, and a control. The canals were obturated using the single cone obturation technique. Dentinal penetration of sealers was measured using Micro-CT at the coronal, middle, and apical portions of root canals.

Results:
Greater sealer penetration was observed with ultrasonic and Lentulospiral agitation compared to GP-mediated ultrasonic agitation for both sealers.

Conclusion:
BIO-C yielded better results with ultrasonic and Lentulospiral agitation, while AH Plus performed better with ultrasonic agitation.
Assessment of Dental Anxiety in Visually Impaired Children Using Two Methods of Oral Health Education in Nizamabad District: A Randomized Control Trial
Authors: Nikhitha Dasari , Ratnaditya Akurathi , Anusha Challagulla , Anusha Karre , Srilekha Macharla , Harshavallika Beeram 
Published: January 2025

ABSTRACT

Background:
Oral health is greatly impacted by visual impairment, which raises the possibility of dental treatment anxiety. Therefore, the goal of this research is to assess dental anxiety in children who are blind.

Aim and Objectives of the Study:
The intention of the study was to raise awareness of oral health among visually impaired children and to use the RMS-tactile scale (TS) to measure their anxiety levels.

Materials and Methods:
In the current study, 50 students with impairment of vision were split into two groups for a randomized control trial. There were 25 children in each group. Group 1 received dental health instruction using Braille, while Group 2 received it via audio tactile performance (ATP) approach. Pre- and post-treatment anxiety levels were recorded using RMS-TS for both groups, and scores for the plaque index (PI) and gingival index (GI) were also computed from baseline and three-month evaluations were conducted.

Results:
We performed a statistical study using a paired t-test and a one-way ANOVA. In the evaluation of pre-anxiety level on RMS-TS, group 2 (ATP) had high significance, and the values for the GI (P < 0.032) and PI (P < 0.020) decreased statistically.

Conclusion:
According to the present study, using a combination of audio and tactile models to explain dental procedures to visually impaired children can effectively reduce their anxiety.
In Silico Molecular Docking of Ocimum sanctum Phytochemicals: Targeting Key Biomarkers in Oral Cancer
Authors: Umapathy Vidhya Rekha , Swamikannu Bhuminathan , Natarajan Prabhu Manickam , Devi Renuka , Nandini MS , Vimalarani  , Vijayalakshmi Periyasamy 
Published: January 2025

ABSTRACT

Background:
Oral cancer is the sixth most common cancer occurring anywhere in the globe. Cancers of the mouth are caused by tobacco and alcohol overuse, and also between pre-malignancy and full-fledged malignancy. In order to avoid the onset of illness, proper cleanliness of the mouth is important.

Objective:
In this study we aimed to identify the potential inhibitor for Aurora kinase B as a potential therapeutic target for oral cancer from the phytocompounds from Ocimum sanctum.

Methodology:
Compounds from Ocimum sanctum were screened against Aurora kinase B using a structure-based virtual screening approach using Pyrx software. Based on the scoring parameters best compounds were chosen to analysis the interaction by using Discovery studio visualizer.

Results:
Four compounds—pedunculin, nevadensin, chrysoeriol, and genistein were selected as the most promising leads based on the scoring parameter.

Conclusion:
These chemicals could be potential therapeutic candidates that need to be investigated further in the laboratory.
Effect of Polydopamine Nanoparticle-Induced Surface Modification on Demineralized Dentin for Enhanced Functional Remineralization
Authors: Madhubala Manavalan Madhana , Mahalaxmi Sekar 
Published: January 2025

ABSTRACT

Background:
Development of biomimetic nanomaterials for remineralization therapy is an important strategy in minimally invasive dentistry. The size of the therapeutic agent has a great influence on bioavailability, penetration ability, and larger surface area for interaction in hard tissue remineralization through the nonclassical crystallization pathway. This study aimed to comparatively evaluate the remineralization-inducing potential of polydopamine (PDA) and polydopamine nanoparticles (nPDs) on artificially demineralized dentin.

Methodology:
nPDs were prepared using commercially procured dopamine hydrochloride by the wet precipitation method. Obtained particles were characterized using scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM-EDX), transmission electron microscopy, particle size analysis and zeta potential determination via dynamic light scattering with a laser particle analyser, X-ray diffraction, X-ray photoelectron spectroscopy, and attenuated total reflectance Fourier transform infrared spectroscopy. The cytocompatibility of nPD and PDA was assessed by (3-(4,5-dimethythiazol-2-yl) 2,5-diphenyl tetrazolium bromide) assay on human dental pulpal stem cell lines. A total of 66 demineralized dentin slabs of 3 mm × 3 mm × 1 mm dimension were immersed in 2 mg/ml of freshly prepared PDA and nPD solutions for 12 hours, followed by reimmersion in calcium and phosphate solution at 37°C days for 10 days. Samples immersed in deionized water were used as a control group. All the specimens were subjected to various remineralization testing methods using SEM-EDX, Vicker’s microhardness, and micro-Raman spectroscopic analysis. The Shapiro–Wilk test was used to assess the normality of the distribution, followed by one-way ANOVA for intergroup analysis and post
hoc Tukey’s test for multiple comparisons within groups.

Results:
nPD exhibited a nanospherical morphology with positive zeta potential. nPD revealed the polymeric amorphous nature with characteristic functional groups and exhibited >80% relative cell viability. nPD promoted superior remineralization ability by the formation of hydroxyapatite closely mirroring the Ca/P ratio of natural dentin with a hardness value significantly closer to the baseline, which was further evidenced by higher-intensity peaks of phosphate, amide, and proline on micro-Raman spectroscopic analysis.

Conclusion:
Dentin remineralization is more strongly facilitated by nanometric PDA than PDA, which has an enhanced functional remineralization effect.
Accuracy and Reliability of Oral Exfoliative Cytology in Age Estimation: An Observational Study
Authors: Shaik Naseemoon , Anne Vivek , Mantha Saraswathi Saisupraja , Reddy Medapati Murali Jogi , Priyadarshini Vidya , Tanuja B , Pudi Sriharsha 
Published: January 2025

ABSTRACT

Aim and Objectives:
Age and gender identification are critical in forensic investigations, especially for unidentified human bodies. This study explores differences in nuclear size, nuclear area, and nuclear-cytoplasmic ratio between healthy individuals and those with physiological or pathological conditions, aiming to understand how these variations may influence age estimation.

Materials and Methods:
Following ethical clearance, the study was conducted on 80 participants aged 20–35 years, who were divided into four groups of 20 each, Group 1: Healthy individuals; Group 2: Individuals with physiological variations; Group 3: Individuals with pathological variations; and Group 4: Individuals with acquired conditions. Buccal cell samples were collected using a moistened tongue blade. Measurements of cell size, nuclear dimensions, nuclear area, and the nuclear-cytoplasmic ratio were taken.

Results:
Significant differences in nuclear and cell sizes were observed among participants with acquired conditions and metabolic disorders compared to healthy controls.

Conclusion:
The study suggests that systemic diseases and tobacco use (without visible oral lesions) can significantly affect cellular morphology, which may have implications for the accuracy of age determination in forensic analyses.
Assessment of IL- 35 Gene Polymorphism in Periodontitis Patients with and without Diabetes - A Cross-Sectional Study
Authors: Durga J Sri Vishnu , Devi R Renuka , Sonika S , Vignesh T 
Published: January 2025

ABSTRACT

Aim:
To evaluate the IL-35 Gene Polymorphism in Periodontitis subjects with and without type 2 diabetes mellitus.

Methodology:
96 participants were categorized into three groups, Group 1: Systemically and Periodontally healthy individuals, Group 2: Systemically healthy patients with Generalized chronic periodontitis, Group 3: Generalized chronic periodontitis with type 2 diabetes Mellitus. Genomic DNA was extracted from the whole venous blood samples collected from the participants using The PureLink Genomic Kit. The region spanning rs1580809257 and rs1580801731 were amplified using appropriate primer and reaction conditions. Determination of genotypes for each of the polymorphisms assessed using Amplification Refractory Mutation System (ARMS) - based PCR and Restriction Fragment Length Polymorphism (RFLP). The distribution of genotype and allele frequencies for Case and control groups were compared using the Chi-square test. Hardy Weinberg equilibrium is calculated.

Results:
The gene and allele frequency were found to be similar in all three groups.

Conclusion:
The study reveals that polymorphism of rs1580809257 and rs1580801731 in the IL-35 gene is neither associated with Periodontitis patients nor Periodontitis with Diabetes patients.
Renin Angiotensin Aldosterone System Gene Polymorphisms among Type 2 Diabetic Patients with Retinopathy and Nephropathy
Authors: Pawar Vaishali S. , Datkhile Kailas D. , Sontakke Ajit V. , Pawar Satyajeet K. , Patil Prajakta S. 
Published: January 2025

ABSTRACT

Introduction:
Diversified pathways are associated with the advancement of type 2 diabetes mellitus (T2DM) towards retinopathy and nephropathy. Experimental data have suggested the role of renin-angiotensin-aldosterone system (RAAS) gene polymorphism in diabetic nephropathy (DN) patients. Since very few combined studies were done for RAAS genes in T2DM patients with DN and diabetic retinopathy (DR), we tried to explore the association of certain RAAS gene polymorphisms in them.

Methods:
Single nucleotide polymorphisms (SNPs), Met235Thr of angiotensinogen (AGT) gene, and insertion/deletion (ins/del) of angiotensin-converting enzyme (ACE) gene from RAAS pathway were studied using polymerase chain reaction (PCR) method in this cross-sectional study. T2DM patients diagnosed with nephropathy and retinopathy were selected (n = 125 each) consecutively and compared with T2DM patients without DN and/or DR. SPSS software was used for statistical analysis.

Results:
Considerably higher blood pressure, serum urea, and creatinine levels were found among DN patients. The DD genotype of ACE SNP was significantly higher in the DR and DN groups (OR: 2.87,95%CI: 1.48-5.56, P = 0.002 and OR: 2.36,95%CI: 1.23-4.53, P = 0.010) compared to the DM Group. In AGT Met235Thr SNP, the CC and TC genotypes were significantly increased in DN while only the TC genotype was significantly increased in the DR group.

Conclusion:
ACE ins/del and AGT Met235Thr SNPs were significantly related to the risk of development of nephropathy and retinopathy in T2DM patients.
Assessment of Reporting Quality in Orthodontic Systematic Reviews: An Observational Study
Authors: Alharbi Fahad , Alghabban Rawda O. 
Published: January 2025

ABSTRACT

Objective:
This study aimed to assess the reporting quality of orthodontic systematic reviews published between 2018 and 2022.

Methods:
A retrospective observational study was conducted, analyzing 2114 articles from selected orthodontic journals. Descriptive statistics were used to summarize the characteristics of the included systematic reviews. Compliance scores were calculated for each review, and the overall compliance score was determined.

Results:
The European Journal of Orthodontics (EJO) contributed the most systematic reviews (49%), followed by the Journal of Orthodontics (JO, 8%). Europe had the highest first-author proportion (42%), while Asia accounted for 26%. The majority of reviews involved 4-6 authors (59%). The overall compliance score was 79.2% (95% CI: 78.1-80.3). EJO had the highest compliance (79.9%, 95% CI: 78.3-81.5), followed by Angle Orthodontist (78.9%, 95% CI: 76.8-81.1). Compliance scores varied across regions, with Europe scoring the highest (79.7%, 95% CI: 77.9-81.6). Reporting key Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist items, such as meta-analysis methods and reporting bias assessment, was inadequate.

Conclusion:
The reporting quality of orthodontic systematic reviews varied, highlighting the need for improvement in areas such as reporting meta-analysis methods and addressing reporting bias. Adherence to the PRISMA guidelines should be emphasized to enhance transparency and strengthen the credibility of orthodontic systematic reviews, supporting evidence-based decision-making in orthodontic practice.
Oral Health of the Electronic Cigarette Smokers: A Systematic Review
Authors: Alwafi Abdulraheem A. , Abuljadayel Layla W. 
Published: January 2025

ABSTRACT

Background:
This review aimed to evaluate the impact of e-cigarette usage on oral health, given the rising popularity of e-cigarettes and their perceived lower risk compared to traditional smoking.

Materials and Methods:
Searches were conducted in PubMed, Cochrane, and Scopus databases for studies from January 2014 to December 2023. Eligibility was based on the PICOS criteria. Data extraction was performed by two independent investigators. Studies were assessed using the Effective Public Health Practice Project (EPHPP) Quality Assessment Tool.

Results:
The review included 31 studies, primarily from the USA and Saudi Arabia: 12 cross-sectional, nine case-control, five prospective observational, and five RCTs. Findings showed that clinical periodontal conditions and pro-inflammatory marker levels in e-cigarette users were similar to non-users and lower than conventional cigarette smokers. Dental issues studied included fractures, pain, decay, sensitivity, and loss. Two studies examined harmful metabolites in e-cigarette users. Most studies had low-quality evidence, relying on self-reported data without considering other influencing factors.

Conclusion:
E-cigarette users may experience more changes in oral tissues compared to non-smokers and former smokers.
Knowledge and Perception of Artificial Intelligence Amidst the Health Professionals—A Web-Based Survey
Authors: Jena Amrit , Chaudhary Neel , Manohar Balaji , Mahapatra Niva , Dubey Deepa , Sharma Ankita , Bhuvaneshwari S. 
Published: January 2025

ABSTRACT

Introduction:
Artificial intelligence (AI) has been a revolutionary jump in the field of dentistry and medicine.

Materials and methods:
The acceptance of the AI among healthcare professionals is growing minutely.

Results:
This study identifies the knowledge and perception of the health professionals about the implementation of AI in regular practice.
Role of Intravenous Pyelography in the Evaluation of Developmental Renal Anomalies
Authors: Gokul V Ganesh , Ayyadurai Saranya , Sarvesh R , Thangam Vinoth 
Published: January 2025

ABSTRACT

Aim:
This study aims to examine the role of intravenous pyelography (IVP) in the evaluation of developmental renal anomalies.

Materials and Method:
A prospective cross-sectional study was conducted on 26 subjects presenting with loin pain and suspicion of renal anomalies, subjected to IVP at ACS Medical College and Hospital, Chennai. Descriptive statistics were used for frequency and percentage.

Results:
The study population consisted of 61.5% males and 38.5% females, indicating a male predilection for renal anomalies. Horseshoe kidney (31%) and duplex collecting system (23%) were the most prevalent anomalies, underscoring the importance of early detection and tailored management. Less frequent anomalies included malrotation, ectopic kidney, crossed renal ectopia, and unilateral renal agenesis.

Conclusion:
Despite advancements in imaging technology, IVP remains relevant for detailed anatomical and functional assessment of the urinary tract. This study highlights the importance of proactive management and multidisciplinary collaboration in optimizing outcomes for patients with developmental renal anomalies.
Role of Diagnostic Accuracy of Diffusion-Weighted Imaging In Adult Posterior Fossa Tumors – A Prospective Cross-Sectional Study
Authors: Keerthana B , Moorthy M Janani , Sarvesh R , Thangam Vinoth 
Published: January 2025

ABSTRACT

Aim:
This study aims to investigate the role of diffusion-weighted imaging (DWI) in enhancing the diagnostic accuracy of adult posterior fossa tumors.

Materials and Methods:
A prospective cross-sectional study was conducted involving 20 patients with suspected CNS posterior fossa tumors. MRI scans were performed using a 1.5 Tesla machine with appropriate sequences, including DWI. Data analysis was performed using descriptive statistics.

Results:
The study revealed that the majority of posterior fossa tumors occurred in the age group of 61–70 years, with medulloblastoma being the most common tumor subtype. Male patients were more prevalent than females, and DWI showed hyperintensity in the majority of lesions. The reported sensitivity and specificity of DWI were high, indicating its effectiveness in tumor evaluation.

Conclusion:
In conclusion, this study provides valuable insights into the epidemiology, clinical features, and radiological characteristics of adult posterior fossa tumors. It underscores the significance of advanced imaging techniques like DWI in improving diagnostic accuracy and guiding clinical management decisions for these challenging neoplasms.
An In vitro Study Comparing Dimensional Stability of Four Types of Interocclusal Recording Material
Authors: Shetty Aksshay , Saleem C Mohammed , Ajmal B Mohammed , Afzaa S A Saadath , Saleem Nawal , Raheel Syed A. 
Published: January 2025

ABSTRACT

Background and Objectives:
Interocclusal recording materials should have high dimensional stability for accurate articulation. The aim of this in vitro investigation is to assess and compare the linear dimensional stability of interocclusal recording materials over time.

Methods:
Polyvinylsiloxane, polyether, bisacryl and bite registration wax were used in the study. The test used a mold according to the American Dental Association (ADA) specification number 19. A total of 40 samples were collected, and each group consisted of 10 samples. The dimensional changes in the samples after 1, 24, 48, and 72 h were measured using an optical microscope equipped with a micrometer device.

Results:
Each sample had five measurements taken at different times, and the mean of those readings was used to calculate the dimensional change and compare it to the initial measurement made on the die. ANOVA and the Tukey HSD test for repeated measurements were used to examine the data. The average percentage dimensional change over time 1) Group A: 1, 24, 48, and 72 h; 2) Group B: 1, 24, 48, 72 h; 3) Group C: 1, 24, 72 h; 4) Group D1, 24, 48, 72 h; and so on.

Interpretation and Conclusion:
Both material and temporal considerations impact on linear dimensional stability. The most dimensionally stable material was discovered to be polyvinylsiloxane, followed by polyether, bisacryl, and bite registration wax.
Hepatitis B: Assessment of Knowledge, Attitude, and Practice among the Graduating Dental Students of Odisha: A Web-Based Survey
Authors: Kashyap Shubham , Manohar Balaji , Bali Anmol K. , Sharma Nitin , Dhiman Meenu , Singh Ajaypal , Mishra Pratyush , Bhuvaneshwari S. 
Published: January 2025

ABSTRACT

Introduction:
Evaluating the level of knowledge, attitude, and practice regarding Hepatitis B viral infection among the interns of dental colleges in Odisha.

Materials and Method:
A questionnaire with 20 closed-ended questions was circulated to 269 interns in the dental colleges of Odisha. A total of 243 interns participated in the study and responded on various aspects of Hepatitis B viral infection including symptoms, transmission mechanisms, preventive measures, and immunization.

Results and Conclusion:
The findings revealed that while most dental interns had a basic understanding of Hepatitis B viral infection, significant gaps existed in their knowledge of transmission routes and the importance of vaccination.
Antibacterial Properties of Lemon Juice–Mediated Zinc Oxide Nanoparticle and Titanium Dioxide Nanoparticle
Authors: Missier Mary Sheloni , Ramakrishnan Mahesh , Veerasankar S , Dhanalakshmi C , Ponniah Harish , Rajeshkumar Shanmugam 
Published: January 2025

ABSTRACT

Introduction:
The emergence of antibiotic-resistant pathogenic bacteria has become a major global health concern, with the increasing prevalence of infections that are difficult to treat with conventional antibiotics. As a result, there is a critical need for alternative antimicrobial agents that can effectively control bacterial infections and combat the growing problem of antibiotic resistance.

Materials and Methods:
In this study, the antibacterial properties of lemon juice mediated zinc oxide nanoparticles (ZnO NPs) and titanium dioxide nanoparticles (TiO2 NPs) was evaluated against oral pathogens. The synthesized nanoparticles were characterized using UV-Vis spectroscopy. The antimicrobial activity of the nanoparticles was determined by the agar well diffusion assay.

Results:
Our results showed that both ZnO NPs and TiO2 NPs exhibited potent antimicrobial activity against both Streptococcus mutans and Streptococcus mitis. Our findings suggest that the antimicrobial activity of ZnO NPs and TiO2 NPs is attributed to the disruption of bacterial cell membrane integrity and inhibition of bacterial growth.

Conclusion:
Therefore, lemon juice mediated ZnO NPs and TiO2 NPs can be considered as promising antimicrobial agents for controlling bacterial infections.