bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Legislative Adjustment of Chinese Enforcement Regulation of the International Commercial Settlement Agreement in the context of the Singapore mediation convention
Authors: Xiong Hailiang 
Published: January 2025

Abstract
The United Nations Convention on International Settlement Agreements Resulting from Mediation has established an external legal framework for the cross-border enforcement of international commercial mediation settlement agreements. By virtue of the two-aspect institutional design, this Convention has constructed a modern mechanism for the direct enforcement of international commercial mediated settlement agreements. As one of the first signatories, China does not yet have the specific mediation legislation to conform to the Convention and cannot channel the Singapore Convention. This article discussed the dilemma of the ratification of the Singapore Convention in China from a comparative perspective and argues that China should adjust its mediation regulation by reducing the requirement of application for enforcement and limiting the judicial supervision to establish a direct enforcement mechanism following the potential international obligation under the Singapore Convention and further refine the macro mediation system.
Tiaogan Jiejiu Tongluo formula alleviates hepatic steatosis in NAFLD mice by regulating AMPK signaling pathway
Authors: Lei Yuting , Wang Lianping , Song Linze , Han Jiajun , Ma He , Luo Haoming , Ma Yan , Han Dong 
Published: January 2025

Abstract

Objectives
Tiaogan Jiejiu Tongluo formula (TJTF) is a traditional Chinese medicine formula for liver disease. The purpose of this study was to explore the protective effects and mechanisms of TJTF on nonalcoholic fatty liver disease (NAFLD) mice.

Methods
A NAFLD model of mice was established by a combination of a high-fat diet and CCL4 and then treated with TJTF. The damage to liver tissue was observed through histopathology, and the levels of AST, ALT, TG, TC, LDL-C, and HDL-C in the serum, as well as SOD, GSH, and MDA in the liver tissue were determined using biochemistry or ELISA kit. The expression of proteins related to the AMPK pathway was detected by western blotting.

Results
Biochemical indicators and pathological examination showed that TJTF could enhance the antioxidant capacity of liver tissue and significantly reduce liver lipid deposition. In addition, TJTF significantly increased levels of LKB1 and p-AMPK, and decreased the levels of HMGCR, SREBP-1c, FAS, and ACC.

Conclusion
TJTF can alleviate hepatic steatosis and effectively improve NAFLD by regulating AMPK signaling pathway in NAFLD mice.
A review of the environmental spread of Salmonella enterica serovars through water in Africa
Authors: Rabiu Akeem Ganiyu , Fadiji Oyinkansola , Adesoji Ayodele Timilehin , Jimoh Simiat Olanike , Cho Sohyun 
Published: January 2025

Abstract
Salmonella is an enteric pathogenic bacterium in mammals that thrives in sewage, soil, and aquatic environments because of its wide ecological adaptability. The spread of Salmonella infection is associated with a lack of clean water, poor hygiene, and poor sanitation in developing countries. However, the input of Salmonella-contaminated surface water and groundwater in the environmental dissemination of antimicrobial-resistant Salmonella is obscure outside developed countries. This study aimed to assess literature that described Salmonella from groundwater, surface water, and treated water in Africa and profiled the bacteria for antimicrobial resistance (AMR). We seek to highlight the pitfalls in S. enterica identification schemes and determine the entry routes of S. enterica into surface water and groundwater to comprehend the spread patterns of S. enterica through the water ecosystem. Salmonella was frequently recovered not only from groundwater but also from unprotected springs and river water in Africa. However, the insufficiency of standard methods limited the speciation of the bacteria and only a few studies determined the isolates’ AMR patterns. Integrating the Salmonella surveillance in environmental and treated water into the global Salmonella surveillance can assist in controlling typhoid fever spread and generally reduce the burden of waterborne infections in Africa.
Conformans novas selves: The significance of Balkan route migrants’ possible and future narrative identities
Authors: Poppi Fabio Indìo Massimo 
Published: January 2025

Abstract
This study explores the multifaceted nature of narrative identities among migrants, emphasizing the importance of possible and future dimensions. Drawing on interviews with fifteen men from Pakistan, Afghanistan, Iraq, Syria, and Bangladesh who traveled the Balkan route to Trieste (Italy), the research examines how temporal influences, narrative disruptions, reverse cultural enrichment, and the significance of revenge shape their identities. Integrating biographical and interactional approaches, the study highlights how migrants use narratives to bridge past experiences with future aspirations, creating coherence in their personal histories. These narrative identities provide psychological resilience, meaning-making, and empowerment, allowing migrants to envision and actively shape their futures. By addressing the underexplored potential and future aspects of narrative identity, this research contributes to a more comprehensive understanding of identity construction in the context of migration, offering insights into the dynamic and transformative power of narratives in shaping personal and social realities.
Impact of an institutional antifungal stewardship program on antifungal usage and outcomes in patients with invasive fungal infections
Authors: Sachdev Janya , Gourav Sudesh , Xess Immaculata , Soneja Manish , Punjadath Sryla , Siddharth Vijaydeep , Pandey Mragnayani , Gupta Sonakshi , Manhas Aish , Rana Bhaskar , Appasami Kavi Priya , Singh Gagandeep 
Published: January 2025

Abstract
Therapeutic and prophylactic use of antifungals is rising continuously. However, inadequate awareness of diagnostic and treatment guidelines and limited laboratory modalities lead to inappropriate use. This study assessed the impact of an institutional antifungal stewardship program on antifungal use practices and patient outcomes. In the pre-intervention phase, data was collected regarding antifungal therapy among patients with invasive fungal infections. Appropriateness of antifungal prescription was assessed. In the intervention phase, simple algorithms for diagnosis and management of fungal infections were prepared from international guidelines and incorporated into a booklet for distribution. Monthly training sessions were conducted. New serological and molecular tests and therapeutic drug monitoring were introduced. In the post-intervention phase, an antifungal stewardship team was constituted for clinical advisory on demand and ongoing training. Data regarding antifungal therapy was collected and compared with pre-intervention data. Untreated patients decreased from 25% to 18.9% post-intervention (P = .28). Appropriate antifungal use increased from 72.6% to 77.9% (P = .4) among patients with a single fungal infection, and from 57.1% to 88.5% (P = .04) for at least one infection among those with dual fungal infections. 49 incidents of inappropriate use in various categories were seen among 75 patients receiving antifungals pre-intervention, decreasing to 42 incidents among 94 patients post-intervention (P = .06), particularly evident among patients with dual infections (P = .002). Mortality increased from 51% to 75.86% post-intervention (P = .0001). Overall, the small improvement noticed in antifungal usage pattern can still be considered significant, given the limited study period.
Evidence for magneto-gravitational processes in supermassive black hole binary PG 1553+113
Authors: Zhang Haiyun , Yan Dahai , Zhang Li , Tang Niansheng 
Published: January 2025

ABSTRACT
PG 1553+113 has drawn significant attention for its quasi-periodic oscillation (QPO) in $\gamma$-ray variability, though the origin of its variability remains uncertain. In this study, we propose a physical mechanism to explain the observed $\gamma$-ray variability within the framework of a supermassive black hole binary (SMBHB) system, supported by a newly identified component hidden in the light curve. A detailed analysis for its $\sim$16-yr light curve obtained from Fermi-LAT observations is performed by Gaussian process. As anticipated, the QPO of 2.1 yr ($771\pm 8$ d) is effectively captured by the stochastically driven damped simple harmonic oscillator kernel within the underdamped regime, and the overall stochastic nature of the variability is described by the damped random walk (DRW) kernel albeit with an unconstrained damping time-scale. Additionally, our results reveal a previously unrecognized component in active galactic nuclei variability, characterized by the Mat$\acute{\rm e}$rn$-3/2$ kernel, which is typically associated with systems undergoing abrupt energy release. These findings can be consistently interpreted within the SMBHB framework. The QPO of $\sim$2.1 yr is likely attributed to the orbital motion in a SMBHB system. The Mat$\acute{\rm e}$rn$-3/2$ component is interpreted as resulting from magnetic reconnection events triggered by gravitational perturbations of the magnetic field within the jet, occurring as one black hole approaches the other. Meanwhile, in this case, the damping time-scale of the common DRW kernel remains unconstrained due to the influence of new perturbations within the system.
The nature and evolution of early massive quenched galaxies in the simba-C simulation
Authors: Szpila Jakub , Davé Romeel , Rennehan Douglas , Cui Weiguang , Hough Renier T 
Published: January 2025

ABSTRACT
We examine the nature, origin, and fate of early ($z\ge 2$) massive ($M_\star \gt 10^{10}\,\mathrm{ M}_\odot$) quenched galaxies (EQGs) in a new $(100\,h^{-1}\,{\rm Mpc}^3)$ run of the simba-C galaxy formation model. We define ‘quenched’ to be $\gt 4\sigma$ below an iterative polynomial fit to the star-forming sequence (SFS), and find that simba-C produces EQGs as early as $z\sim 5$ and number densities agreeing with observations at $z\lesssim 3$ (though slightly low at $z\gtrsim 4$). Using a photometric-based EQG selection or a fixed specific star formation rate cut of $10^{-10}$ yr$^{-1}$ yields similar results. EQGs predominantly arise in central galaxies with stellar mass $M_\star \sim 10^{10.5-11.3}\,\rm{M}_\odot$, not necessarily the most massive systems. A Uniform Manifold Approximation and Projection shows that quenched galaxies have notably large black hole-to-stellar mass ratios, lower rotational support, and less dust, but are not atypical versus similar-mass non-EQGs in their environments, halo mass, or halo gas temperatures at the time of quenching. However, via galaxy tracking we show that the progenitor environments of EQGs are significantly more overdense than that of non-EQGs, which drives higher black hole mass fractions and stellar-to-halo mass ratios. This results in the Eddington ratio dropping sufficiently low for simba-C’s jet mode feedback to turn on, which quickly quenches the host galaxies. EQGs thus seem to be galaxies that grow their black holes quickly within highly dense environments, but end up in moderately dense environments where black hole feedback can quench effectively. We find that $\gtrsim 30~{{\ \rm per\ cent}}$ of EQGs rejuvenate, but the rejuvenating fraction drops quickly below $z\lesssim 2$. By $z=0$, it is difficult to distinguish the descendants of EQGs versus non-EQGs.
Comprehensive analysis of four Kepler eclipsing binaries possessing solar-type components on wide eccentric orbits
Authors: Özdarcan Orkun , Çakırlı Ömür 
Published: January 2025

ABSTRACT
Such spectroscopic and photometric studies have opened up a window into the interiors of solar-type stars that are found in eclipsing binaries, which is proving to be a remarkable facility of knowledge to test models of stellar evolution. Until recently, numerous cases had been analysed using Kepler data. To further clarify this research, we investigate four long-period detached eclipsing binaries: KIC 8868907, KIC 8610483, KIC 9948002, and KIC 11769146. Our findings revealed that the target systems are composed of solar-type components in a wide and very eccentric orbit. Simultaneous orbital and light-curve solutions indicate that the computed stellar radii and masses of the components of KIC 8868907, KIC 9948002, and KIC 11769146 align with theoretical models within the uncertainties. We observe a curious case for KIC 8610483 whose components are very likely main-sequence stars and possess lower mass than our Sun but are positioned very close to the terminal age main sequence due to their significantly large radii compared to a classical main-sequence star with a similar mass. Predictions of circularization theories suggest that none of the target systems will reach a circular orbit before the end of their main-sequence phases.
A case of high tibial osteotomy in a patient with psoriatic arthritis
Authors: Koga Mikiro , Maeyama Akira , Ishimatsu Tetsuro , Hagio Tomonobu , Shibata Terufumi , Yamasaki Yutaro , Sugino Yuki , Yamamoto Takuaki 
Published: January 2025

ABSTRACT
Traditionally, patients with rheumatic diseases, such as rheumatoid arthritis (RA), were considered unsuitable for joint-sparing surgery. In the present study, we report on bilateral knee joints affected by psoriatic arthritis coexisting with osteoarthritis, with good, albeit short-term, results. A 62-year-old woman was treated for psoriatic arthritis with a biologic (adalimumab). The Disease Activity in Psoriatic Arthritis index was 7.24, indicating low disease activity. She had been suffering from bilateral knee pain for some time and was treated conservatively by her local doctor, but the pain persisted, and she came to visit us. At the initial visit, tenderness in the medial joint line of both knees and hydrarthrosis in the right knee were observed. Preoprative radiographs at the time of the initial examination showed medial-type osteoarthritis in both knees. First, interlocking closed wedge high tibial osteotomy (CWHTO) was performed on the right knee. This was followed 1 year later by right knee implant removal and interlocking CWHTO on the left knee, with implant removal on the left knee 1 year after that. In both knees preoperatively and postoperatively, the joint range of motion and the Knee Injury and Osteoarthritis Outcome Score total improved Considering the patient’s background, we considered high tibial osteotomy if the disease activity was controlled. However, if the disease worsens in the future, joint destruction may occur, so careful follow-up is necessary.
Metabolic profiling of meningioma reveals novel subgroup-specific biologic insights and outcome dependencies
Authors: Landry Alexander P , Wang Justin Z , Yefet Leeor S , Liu Jeff , Patil Vikas , Zhang Wen-Jiang , Sosa Julio , Ellenbogen Yosef , Gui Chloe , Ajisebutu Andrew , Aldape Kenneth , Gao Andrew , Kislinger Thomas , Chen Eric X , Nassiri Farshad , Zadeh Gelareh 
Published: January 2025

Abstract

Background
Our group and others have recently identified four molecular groups of meningioma, with unique underlying biology and outcomes. The relevance of group-specific metabolite profiles (particularly among hypermetabolic tumors), has not been explored.

Methods
We performed untargeted metabolic profiling of meningiomas representing each molecular group and World Health Organization (WHO) grade. Prognostic biochemicals were identified using Cox regression and their biological importance was explored using RNA and protein-based pathway analyses. Validation was performed using targeted high performance liquid chromatography-mass spectrometry (HPLC-MS/MS).

Results
Global metabolic profiling identified 560 unique biochemicals. We identified a 21-metabolite outcome signature which is strongly predictive of outcome after adjusting for WHO grade, extent of resection, and receipt of adjuvant radiotherapy (HR = 326.49, 95% CI = 16.72-6375.48, P &lt; .0001). The abundance of N6-trimethyllysine was associated with earlier time to recurrence on our whole cohort (log-rank P = .009) and within hypermetabolic and WHO grade 2 tumors specifically; this was validated using targeted HPLC-MS/MS on two cohorts. Consensus RNA and protein expression analysis demonstrated as association between N6-trimethyllysine abundance and activation of oxidative phosphorylation pathways, which portended worse outcomes in the hypermetabolic subgroup but, interestingly, better outcomes in the proliferative subgroup. By contrast, upregulated pyruvate and lactate transporters were associated with worse outcomes in proliferative meningiomas specifically.

Conclusions
This is the first study to demonstrate a subgroup-specific prognostic role of N6-trimethyllysine in hypermetabolic meningiomas, offering increasingly granular outcome predictions using a widely accessible technique (HPLC-MS/MS). We also suggest fundamental differences in preferred energy utilization between and a potential need for subgroup-specific therapies.
Inter-organelle communication dynamically orchestrates juvenile hormone biosynthesis and female reproduction
Authors: Zhu Shiming , Liu Fangfang , Chen Xiaoyi , Xia Sishi , Wu Yingting , Tang Wei , Ren Chonghua , Wang Jian , Li Sheng 
Published: January 2025

Abstract
Inter-organelle communication coordinates cellular homeostasis and function. Juvenile hormone (JH) is produced in the corpora allata (CA) and acts as a gonadotrophic hormone in most insects. Using transcriptomic, biochemical, molecular, and genetic analyses, here we investigated the underlying mechanism how inter-organelle communication dynamically orchestrates JH biosynthesis and female reproduction in the American cockroach, Periplaneta americana. The extracellular stimuli insulin and allatostatin act through their membrane receptors and antagonistically regulate RyR-mediated Ca2+ release from the endoplasmic reticulum in CA cells. Ca2+-activated CaMKII stimulates energy metabolism in the mitochondria partially via SLC25A6, and induces the expression of JH biosynthetic genes HMGR, Jhamt, and Cyp15a1 through activating transcription factor CREB, which recruits CBP for histone acetylation in the nucleus. Additionally, mitochondria interact with CREB-CBP through mitonuclear communication to regulate JH biosynthesis. From the perspective of inter-organelle communication, this comprehensive study significantly advanced our understanding of hormone biosynthesis and reproductive biology in insects.
The translation initiation factor eIF3M2 upregulates HEAT SHOCK PROTEIN 70 to maintain pollen tube membrane integrity during heat shock
Authors: Kahrizi Zahra , Michailidis Christos , Raabe Karel , Kumar Vinod , Honys David , Hafidh Said 
Published: January 2025

Abstract
Pollen germination and pollen tube (PT) growth are extremely sensitive to high temperatures. During heat stress (HS), global translation shuts down and favors the maintenance of the essential cellular proteome for cell viability and protection against protein misfolding. Here, we demonstrate that under normal conditions, the Arabidopsis (Arabidopsis thaliana) eukaryotic translation initiation factor subunit eif3m1/eif3m2 double mutant exhibits poor pollen germination, loss of PT integrity and an increased rate of aborted seeds. Surprisingly, under HS at 37 °C, eif3m1 pollen germination outperformed wild-type Col-0, showing enhanced PT integrity. We established that the improved thermotolerance of the eif3m1 PT was due to increased expression of its putative paralog eIF3M2, which in turn upregulated Heat Shock protein 70 (HSP70) mRNA and protein levels. Indeed, eIF3M2 overexpression upregulated HSP70 expression, whereas eif3m2 knockdown showed reduced HSP70.1 promoter activity and increased in PT burst under HS conditions. Moreover, we show that eIF3M2 coimmunoprecipitates with HSP70 in PTs and directly interacts with cytoplasmic HSP70.1/2/4 and eIF4G in Nicotiana benthamiana pavement cells. Collectively, our data revealed that plants employ the eIF3M2-HSP70 module as a regulator of thermotolerance to maintain PT membrane integrity and improve fertilization and seed set adaptation under high temperatures.
Phosphorylation-dependent VaMYB4a regulates cold stress in grapevine by inhibiting VaPIF3 and activating VaCBF4
Authors: Yu Qinhan , Zheng Qiaoling , Liu Chang , Zhang Junxia , Xie Yaping , Yao Wenkong , Li Jiaxin , Zhang Ningbo , Hao Xinyi , Xu Weirong 
Published: January 2025

Abstract
Cold stress severely impacts the quality and yield of grapevine (Vitis L.). In this study, we extend our previous work to elucidate the role and regulatory mechanisms of Vitis amurensis MYB transcription factor 4a (VaMYB4a) in grapevine's response to cold stress. Our results identified VaMYB4a as a key positive regulator of cold stress. We demonstrated that VaMYB4a undergoes phosphorylation by V. amurensis calcineurin B-like (CBL) proteins-interacting protein kinase 18 (VaCIPK18) under cold stress, a process that activates VaMYB4a transcriptional activity. Using chromatin immunoprecipitation sequencing (ChIP-seq). We performed a comprehensive genomic search to identify downstream components that interact with VaMYB4a, leading to the discovery of a basic helix-loop-helix transcription factor, V. amurensis phytochrome-interacting factor 3 (VaPIF3). VaMYB4a attenuated the transcriptional activity of VaPIF3 through a phosphorylation-dependent interaction under cold conditions. Furthermore, VaPIF3, which interacts with and inhibits V. amurensis C-repeat binding factor 4 (VaCBF4, a known positive regulator of cold stress), has its activity attenuated by VaMYB4a, which mediates the modulation of this pathway. Notably, VaMYB4a also interacted with and promoted the expression of VaCBF4 in a phosphorylation-dependent manner. Our study shows that VaMYB4a positively modulates cold tolerance in plants by simultaneously downregulating VaPIF3 and upregulating VaCBF4. These findings provide a nuanced understanding of the transcriptional response in grapevine under cold stress and contribute to the broader field of plant stress physiology.
A C4 plant K+ channel accelerates stomata to enhance C3 photosynthesis and water use efficiency
Authors: Alvim Fernanda A L S , Alvim Jonas Chaves , Hibberd Julian M , Harvey Andrew R , Blatt Michael R 
Published: January 2025

Abstract
Accelerating stomatal kinetics through synthetic optogenetics and mutations that enhance guard cell K+ flux has proven a viable strategy to improve water use efficiency and biomass production. Stomata of the model C4 species Gynandropsis gynandra, a relative of the C3 plant Arabidopsis thaliana, are similarly fast to open and close. We identified and cloned the guard cell rectifying outward K+ channel (GROK) of Gynandropsis and showed that GROK is preferentially expressed in stomatal guard cells. GROK is homologous to the Arabidopsis guard cell K+ channel GORK and, expressed in oocytes, yields a K+ current consistent with that of Gynandropsis guard cells. Complementing the Arabidopsis gork mutant with GROK promoted K+ channel gating and K+ flux, increasing stomatal kinetics and yielding gains in water use efficiency and biomass with varying light, especially under water limitation. Our findings demonstrate the potential for engineering a C4 K+ channel into guard cells of a C3 species, and they speak to the puzzle of how C4 species have evolved mechanisms that enhance water use efficiency and growth under stress.