bluuebunny/crossref_metadata_2025_split huggingface dataset:
ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas
Authors: Ming Yue , Luo Chunyuan , Ji Beihong , Cheng Jian 
Published: October 2023

Abstract
Background
Gliomas are the most common malignant brain tumors, with powerful invasiveness and an undesirable prognosis. Actin related protein 2/3 complex subunit 5 (ARPC5) encodes a component of the Arp2/3 protein complex, which plays a significant role in regulating the actin cytoskeleton. However, the prognostic values and biological functions of ARPC5 in gliomas remain unclear.

Methods
Based on the TCGA, GEO, HPA, and UALCAN database, we determined the expression of ARPC5 in glioma. The results were verified by immunohistochemistry and Western blot analysis of glioma samples. Moreover, Kaplan-Meier curves, ROC curves, Cox regression analyses, and prognostic nomograms were used to observe the correlation between the ARPC5 expression and the prognosis of glioma patients. GO and KEGG enrichment analyses were conducted to identify immune-related pathways involved with the differential expression of ARPC5. Subsequently, the TCGA database was used to estimate the relationship between ARPC5 expression and immunity-related indexes, such as immune scores, infiltrating immune cells, and TMB. The TCIA database was used to assess the correlation between ARPC5 with immunotherapy. The association between ARPC5 and T cells marker CD3 was also evaluated through immunohistochemistry methods. The correlation between ARPC5 and T cell, as well as the prognosis of patients, was also evaluated using immunological methods. Moreover, the effect of ARPC5 on the biological characteristics of LN229 and U251 cells was determined by MTT, clone formation, and transwell migration assay.

Results
The high degree of ARPC5 was correlated with worse prognosis and unfavorable clinical characteristics of glioma patients. In the analysis of GO and KEGG, it is shown that ARPC5 was strongly correlated with multiple immune-related signaling pathways. The single-cell analysis revealed that ARPC5 expression was increased in astrocytes, monocytes and T cells. In addition, ARPC5 expression was strongly associated with immune scores, infiltrating immune cells, TMB, MSI, immune biomarkers, and immunotherapy. In experimental analysis, we found that ARPC5 was significantly overexpressed in gliomas and closely correlated with patient prognosis and CD3 expression. Functionally, the knockout of ARPC5 significantly reduced the proliferation and invasion of LN229 and U251 cells.

Conclusions
Our study revealed that the high expression level of ARPC5 may serve as a promising prognostic biomarker and be associated with tumor immunity in glioma.
Development and psychometric properties of Health-Promoting Lifestyle Scale in Colorectal Cancer Survivors (HPLS-CRCS): a mixed-method study
Authors: Ramezanzade Tabriz Elahe , Ramezani Monir , Heydari Abbas , Aledavood Seyed Amir , Jamali Jamshid 
Published: October 2023

Abstract
Background
Detecting a health-promoting lifestyle in colorectal cancer (CRC) survivors is of paramount importance to manage disease complications, prevent their recurrence, and enhance survival; however, no specialized tool has yet been provided to measure the lifestyle of these patients. Accordingly, this study aimed to develop and determine the psychometric properties of the Health-Promoting Lifestyle Scale in CRC Survivors (HPLS-CRCS).

Methods
This study was a mixed study with an exploratory sequential design in two phases. Concept analysis was performed in the first phase according to Schwartz-Barcott and Kim’s (2000) hybrid model to explain the concept, identify dimensions, and generate items. In the second phase, psychometrics including validity (face, content, and construct) and reliability (internal consistency and stability) were determined. Responsiveness, interpretability, ease of use, item weighting, and scale scoring were also determined.

Results
After explaining the concept, an initial scale encompassing 211 items was developed, content and item analyses were conducted, and the items decreased to 89 items after the face validity assessment. For construct validity, confirmatory factor analysis (CFA) was conducted with a sample size of 500 survivors, and convergent validity was performed for the Persian version of the Health-Promoting Lifestyle Profile II (HPLP-II). Accordingly, 80 items were classified into six factors: activity and rest, spiritual growth, health responsibility, nutrition, interpersonal relationships, and psychological management, with RMSEA = 0.055, χ2/df = 2.484, and χ2 = 6816.516. The reliability of the scale was confirmed, Cronbach’s alpha was between 0.865 and 0.928, and the intraclass correlation coefficient (ICC), the standard error of measurement (SEM), the minimal important change (MIC), and the smallest detectable change (SDC) were 0.896, 3.36, 13.86, and 19.87, respectively.

Conclusion
The HPLS-CRCS consists of 80 items in six dimensions and is a valid and reliable scale for evaluating the health-promoting lifestyle in CRC survivors. Using this scale to evaluate the healthy lifestyle in these survivors can lead healthcare providers to detect deficiencies and plan the lifestyle of CRC survivors during the post-treatment period.
Fear of COVID-19: the mediation role between the COVID-19 diagnosis and KAP in Spanish university students
Authors: Cancela Ana , González-Noriega Mar , Visiers Ana 
Published: October 2023

Abstract
Background
Although health public services recommend prevention strategies for COVID-19 some of these recommendations have not been taken seriously by young people. Understanding why some people comply with these recommendations and others do not seem to be crucial in helping public health services to predict behavior and compliance with rules, especially for young people. Previous studies suggest that knowledge, attitudes, and practices (KAP) are useful to assess compliance with the preventive measures and public health policies. Being afraid has also been found to correlate with more engagement with preventive measures. This study aims to assess the KAP and fear of COVID-19 of Spanish university students and to understand the relation between diagnosis, KAP and the level of fear.

Method
Participants of this cross-sectional study were 598 college students (69.4% women) from different Spanish Universities. Data were collected for a month using an online questionnaire through Sphinx iQ2.

Results
Levels of KAP among Spanish students were satisfactory and results suggest the presence of fear among them. More importantly, fear of COVID-19 mediated the impact of the diagnosis on the KAP.

Conclusions
Feeling fear seems to be the mechanism underlying the relationship between diagnosis and KAP. Diagnosis is associated with KAP when the diagnosis it is accompanied by measures of fear. KAP, diagnosis, or perceived fear of COVID must be taken together in consideration for health interventions and public health campaigns design.
Breast cancer awareness among Sudanese females in Omdurman city: 2021
Authors: Rafat Munir Lawis Munir , Sabir Mustafa 
Published: October 2023

Abstract
Background
Breast cancer is the most commonly diagnosed malignancy among women worldwide. In contrast to Europe, it presents generally very late in Africa. As with the rest of Africa, it is the most common primary malignancy of females in Sudan, and generally presents in stage III or IV disease. This study is intended to analyse the level of breast cancer awareness among women of Sudan.

Objective
To assess the level of awareness of breast cancer among Sudanese females, and their attitude towards breast changes. Also, to establish possible associations between several variables: (education level, age, contact with a breast cancer patient, residence) on awareness.

Methodology
A descriptive cross-sectional community-based study of 385 females in Omdurman city, selected by convenience sampling. An interview based Arabic version of Breast Cancer Awareness Measure (BCAM) was used. Data was coded and analysed using Statistical Package for Social Sciences (SPSS) v.20.

Results
A total of 385 females were included, of which 38.7% were 20–30 years, 47.8% were single, 53.8% had university education, 41% of them were currently unemployed, and 54.3% resided in Ummbadda’s locality. When asked about the signs and risk factors of breast cancer, 55.06% and 55.8% failed to name any. The cumulative percentage of correct answers of close-ended question about signs, risk factors and Federal Ministry of Health (FMoH) screening program were 42.8%, 30.7% and 44.7% in that order. Only 38.2% knew the right method BSE, 48.2% of them rarely practiced it. 38.2% noticed a change in the breast tissue but didn’t visit a doctor. The majority of them, though, said they would see a doctor if they noticed a change in the future.

Conclusion
There is a severe lack of awareness of breast cancer among females of the Sudanese society. Also, there was a clear ignorant attitude practiced by a significant proportion of the candidates.

Recommendations
Further research, to address the limitations of this study is to be done. FMoH has to improve its media massage and arrange targeted awareness campaigns.
Bricks and mortar of well-being: exploring the housing-health connection
Authors: Mehdipanah Roshanak , Weinstein Lara C. 
Published: October 2023

AbstractHousing is a determinant of health. Increasing housing costs and worsening housing conditions have impacted populations worldwide. This Editorial aims to examine the dynamic connection between housing and health and the role of public health in addressing this relationship for a Collection launched at BMC Public Health on Housing as a determinant of health and well-being.
Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model
Authors: Shi Lu , Zhao Tiantian , Huang Lei , Pan Xiaomin , Wu Tianzhen , Feng Xin , Chen Taoli , Wu Jiamin , Niu Jianlou 
Published: October 2023

Abstract
Background
The major safety concern of the clinical application of wild type FGF19 (FGF19WT) emerges given that its extended treatment causes hepatocellular carcinoma. Therefore, we previously generated a safer FGF19 variant - FGF19ΔKLB, which have same effects on glycemic control and bile acid production but much less mitogenic activity. However, it remains unclear as to whether FGF19ΔKLB ameliorates intrahepatic cholestasis.

Results
We found that, similar to that of FGF19WT, the chronic administration of FGF19ΔKLB protects mice from cholestatic liver injury in these two models. The therapeutic benefits of FGF19ΔKLB on cholestatic liver damage are attributable, according to the following mechanistic investigation, to the reduction of BA production, liver inflammation, and fibrosis. More importantly, FGF19ΔKLB did not induce any tumorigenesis effects during its prolonged treatment.

Conclusions
Together, our findings raise hope that FGF19ΔKLB may represent a useful therapeutic strategy for the treatment of intrahepatic cholestasis.
Impact of the adjacent bone on pseudarthrosis in mandibular reconstruction with fibula free flaps
Authors: Steffen Claudius , Soares Ana Prates , Heintzelmann Thelma , Fischer Heilwig , Voss Jan Oliver , Nahles Susanne , Wüster Jonas , Koerdt Steffen , Heiland Max , Rendenbach Carsten 
Published: October 2023

Abstract
Background
Mechanical and morphological factors have both been described to influence the rate of pseudarthrosis in mandibular reconstruction. By minimizing mechanical confounders, the present study aims to evaluate the impact of bone origin at the intersegmental gap on osseous union.

Methods
Patients were screened retrospectively for undergoing multi-segment fibula free flap reconstruction of the mandible including the anterior part of the mandible and osteosynthesis using patient-specific 3D-printed titanium reconstruction plates. Percentage changes in bone volume and width at the bone interface between the fibula/fibula and fibula/mandible at the anterior intersegmental gaps within the same patient were determined using cone-beam computed tomography (CBCT). Additionally, representative samples of the intersegmental zones were assessed histologically and using micro-computed tomography (µCT).

Results
The bone interface (p = 0.223) did not significantly impact the change in bone volume at the intersegmental gap. Radiotherapy (p < 0.001), time between CBCT scans (p = 0.006) and wound healing disorders (p = 0.005) were independent risk factors for osseous non-union. Preliminary analysis of the microstructure of the intersegmental bone did not indicate morphological differences between fibula–fibula and fibula–mandible intersegmental bones.

Conclusions
The bone interface at the intersegmental gap in mandibular reconstruction did not influence long-term bone healing significantly. Mechanical and clinical properties seem to be more relevant for surgical success.
Border-associated macrophages promote cerebral amyloid angiopathy and cognitive impairment through vascular oxidative stress
Authors: Uekawa Ken , Hattori Yorito , Ahn Sung Ji , Seo James , Casey Nicole , Anfray Antoine , Zhou Ping , Luo Wenjie , Anrather Josef , Park Laibaik , Iadecola Costantino 
Published: October 2023

AbstractBackgroundCerebral amyloid angiopathy (CAA) is a devastating condition common in patients with Alzheimer’s disease but also observed in the general population. Vascular oxidative stress and neurovascular dysfunction have been implicated in CAA but the cellular source of reactive oxygen species (ROS) and related signaling mechanisms remain unclear. We tested the hypothesis that brain border-associated macrophages (BAM), yolk sac-derived myeloid cells closely apposed to parenchymal and leptomeningeal blood vessels, are the source of radicals through the Aβ-binding innate immunity receptor CD36, leading to neurovascular dysfunction, CAA, and cognitive impairment.MethodsTg2576 mice and WT littermates were transplanted with CD36−/−or CD36+/+bone marrow at 12-month of age and tested at 15 months. This approach enables the repopulation of perivascular and leptomeningeal compartments with CD36−/−BAM. Neurovascular function was tested in anesthetized mice equipped with a cranial window in which cerebral blood flow was monitored by laser-Doppler flowmetry. Amyloid pathology and cognitive function were also examined.ResultsThe increase in blood flow evoked by whisker stimulation (functional hyperemia) or by endothelial and smooth muscle vasoactivity was markedly attenuated in WT → Tg2576 chimeras but was fully restored in CD36−/− → Tg2576 chimeras, in which BAM ROS production was suppressed. CAA-associated Aβ1-40, but not Aβ1-42, was reduced in CD36−/− → Tg2576 chimeras. Similarly, CAA, but not parenchymal plaques, was reduced in CD36−/− → Tg2576 chimeras. These beneficial vascular effects were associated with cognitive improvement. Finally, CD36−/−mice were able to more efficiently clear exogenous Aβ1-40injected into the neocortex or the striatum.ConclusionsCD36 deletion in BAM suppresses ROS production and rescues the neurovascular dysfunction and damage induced by Aβ. CD36 deletion in BAM also reduced brain Aβ1-40and ameliorated CAA without affecting parenchyma plaques. Lack of CD36 enhanced the vascular clearance of exogenous Aβ. Restoration of neurovascular function and attenuation of CAA resulted in a near complete rescue of cognitive function. Collectively, these data implicate brain BAM in the pathogenesis of CAA and raise the possibility that targeting BAM CD36 is beneficial in CAA and other conditions associated with vascular Aβ deposition and damage.Graphical Abstract
Delirium and neuropsychological recovery among emergency general surgery survivors (DANE): study protocol for a randomized controlled trial and collaborative care intervention
Authors: Mohanty Sanjay , Holler Emma , Ortiz Damaris , Meagher Ashley , Perkins Anthony , Bylund Peggy , Khan Babar , Unverzagt Frederick , Xu Hupuing , Ingraham Angela , Boustani Malaz , Zarzaur Ben 
Published: October 2023

Abstract
Background
Delirium is a complex neuropsychiatric syndrome which consists of acute and varying changes in cognition and consciousness. Patients who develop delirium are at increased risk for a constellation of physical, cognitive, and psychological disabilities long after the delirium has ended. Collaborative care models integrating primary and specialty care in order to address patients with complex biopsychosocial needs have been demonstrated to improve outcomes in patients with chronic diseases. The purpose of this study is to evaluate the ability of a collaborative care model on the neuropsychologic recovery of delirium survivors following emergency surgery.

Methods
This protocol describes a multicenter (eight hospitals in three states) randomized controlled trial in which 528 patients who develop delirium following emergency surgery will be randomized to either a collaborative care model or usual care. The efficacy of the collaborative care model on cognitive, physical, and psychological recovery in these delirium survivors will then be evaluated over 18 months.

Discussion
This will be among the first randomized clinical trials in postoperative delirium survivors evaluating an intervention designed to mitigate the downstream effects of delirium and improve the neuropsychologic recovery after surgery. We hope that the results of this study will add to and inform strategies to improve postoperative recovery in this patient group.

Trial registration
ClinicalTrials.gov NCT05373017. Registered on May 12, 2022.
Trends and future projections of liver cancer incidence in Hong Kong: a population-based study
Authors: Ma Tianyou , Wei Xiaohui , Wu Xiaoming , Du Jianqiang 
Published: October 2023

Abstract
Background
Liver cancer remains a significant burden in Hong Kong. We sought to examine trends in liver cancer incidence using 30 years of cancer registry data in Hong Kong. Additionally, we aimed to assess the impact of age, period and birth cohort on liver cancer incidence, provided projections for liver cancer incidence until 2030, and examined the drivers of liver cancer incidence.

Methods
Data on liver cancer incidence were collected from the Hong Kong Cancer Registry (HKCaR). We assessed age, period, and birth cohort effects using age-period-cohort (APC) models. We employed Bayesian APC analysis with integrated nested Laplace approximations to project the future burden of liver cancer in Hong Kong. Furthermore, we attributed the changes in new liver cancer cases to population growth, population ageing, and epidemiological changes.

Results
The study included a total of 51,333 individuals, of whom 39,287 (76.53%) were male. From 1991 to 2020, the age-standardized liver cancer incidence rate in Hong Kong continued declining, while the number of new cases increased significantly, especially among males. The net drift, representing the overall annual percentage change of the age-adjusted rate, was − 3.06% (95% confidence interval [CI]: -3.31% to -2.80%) for males and − 3.85% (95% CI: -4.61% to -3.09%) for females. Local drift, which estimates the annual percentage change over time specific to age group, decreased in all age groups for both sexes, with a more pronounced decrease in younger age groups. The period and cohort risk of developing liver cancer also showed decreasing trends for both sexes. The study projected a decline in liver cancer cases for males but an increase for females in Hong Kong, with an estimated 1,083 cases in males and 710 cases in females by 2030. Demographic decomposition analysis revealed that while population growth and ageing were the main drivers of increased liver cancer cases, epidemiologic shifts mostly offset these factors.

Conclusion
The period and cohort risk of developing liver cancer in Hong Kong declined due to epidemiological changes. Although the age-standardized incidence rates of liver cancer have also declined, demographic and epidemiological factors have led to lower case expectations in males but a likely increase in females. Further research and epidemiological assessment of the disease are needed.
Evaluation of the fate of trace organic compounds using the Level III fugacity model: a case study of Rajbandh open dump site in Khulna City of Bangladesh
Authors: Pangkaj K. M. , Rafizul I. M. , Kraft E. , Berner S. 
Published: October 2023

AbstractKhulna is the 3rd largest city corporation in Bangladesh that produces 450 tons of Municipal solid waste (MSW) per day and dumps the waste in the Rajbandh open dump site. The main focus of this study is to determine the fate of chemicals in different environmental media at the Rajbandh open dump site. To accomplish the aim Level III fugacity model was evaluated which is subject to steady-state partitioning, reaction, advection, and intercompartmental transfer in an evaluative environment consisting of three compartments landfill gas (LFG), leachate, and waste. In this study, the model was implemented for six chemicals Trichloroethylene, Mono-chlorobenzene, 1,4-dichlorobenzene, 1,2,4-trichlorobenzene, Hexachlorobenzene, and Benzene having different physiochemical properties. The result reveals that Benzene was found with the highest fugacity value of 2.03E + 00 Pa in all compartments while Hexachlorobenzene shows the highest value of concentration 2.67E-01 mol/m3 in the waste compartment. Regarding mass distribution, all chemical shows a higher mass remaining in the waste compartment in comparison with the other compartment. The result also reveals that the dominating removal process of a chemical from the system is the reaction process. For all the chemicals almost all the mass is removed from the environment by reaction process rather than advection in LFG and leachate. Finally, the estimated behavior of the selected chemical will help to evaluate the health and ecological hazards at the open dump site and take necessary steps to control the emission of such hazardous chemicals from the open dump site by the authority.
Monitoring of equatorial plasma bubbles using aeronautical navigation system: a feasibility study
Authors: Hosokawa Keisuke , Saito Susumu , Nakata Hiroyuki , Lin Chien-Hung , Lin Jia-Ting , Supnithi Pornchai , Tomizawa Ichiro , Sakai Jun , Takahashi Toru , Tsugawa Takuya , Nishioka Michi , Ishii Mamoru 
Published: October 2023

AbstractIt has long been known that field-aligned irregularities within equatorial plasma bubbles (EPBs) can cause long-range propagation of radio waves in the VHF frequencies such as those used for TV broadcasting through the so-called forward scattering process. However, no attempt has been made to use such anomalous propagations of VHF radio waves for wide-area monitoring of EPBs. In this study, we investigated the feasibility of monitoring of EPBs using VHF radio waves used for aeronautical navigation systems such as VHF Omnidirectional radio Range (VOR). There are 370 VOR stations in the Eastern and Southeastern Asian region that can be potentially used as Tx stations for the observations of anomalous propagation. We have examined the forward scattering conditions of VHF waves using the magnetic field model and confirmed that it is possible to observe the EPB-related anomalous propagation if we set up Rx stations in Okinawa (Japan), Taiwan, and Thailand. During test observations conducted in Okinawa since 2021, no signal has been received that was clearly caused by anomalous propagation due to EPBs. This is simply because EPBs have not developed to high latitudes during the observation period due to the low solar activity. In March 2023, however, possible indications of EPB-related scattering were detected in Okinawa which implies the feasibility of observing EPBs with the current observation system. We plan to conduct pilot observations in Taiwan and Thailand in future to further evaluate the feasibility of this monitoring technique.
Graphical Abstract