bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of hippocampus‐related candidate genes for Alzheimer's disease
Authors: Taguchi Keiko , Yamagata Hidehisa D. , Zhong Wangtao , Kamino Kouzin , Akatsu Hiroyasu , Hata Ryuji , Yamamoto Takayuki , Kosaka Kenji , Takeda Masatoshi , Kondo Ikuko , Miki Tetsuro 
Published: March 2005

AbstractAlzheimer's disease (AD) is a complex multifactorial disease in which many genetic and environmental factors are involved. We performed an association study using 376 AD patients and 376 control subjects. We studied 35 single nucleotide polymorphisms in 35 genes that were significantly downregulated or upregulated only in the AD hippocampus compared with control and found that 9 single nucleotide polymorphisms were associated with AD. Our data indicated that single nucleotide polymorphisms could highly reflect differences in gene expression. Furthermore, an intronic polymorphism (+9943T/C) in POU2F1 was most significantly associated with AD (p = 0.0007). Our results suggest that POU2F1 is a candidate gene for AD. Ann Neurol 2005;57:585–588
Early onset neuropathy in a compound form of Charcot–Marie–Tooth disease
Authors: Meggouh Farid , de Visser Marianne , Arts Willem F. M. , De Coo Rene I. F. M. , van Schaik Ivo N. , Baas Frank 
Published: March 2005

AbstractA 2‐year‐old boy presented with early‐onset Charcot–Marie–Tooth disease (CMT). His parents had not been diagnosed previously with CMT, but on careful examination they showed clinical signs of CMT and reduced nerve conduction velocities. Genetic analysis identified the boy as a heterozygote for both a peripheral myelin protein 22 (PMP22) duplication and a mutation in the lipopolysaccharide‐induced‐tumour‐necrosis‐factor‐α‐factor (LITAF) gene, whereas each parent only had one mutated CMT gene. This suggests that LITAF mutations can severely affect the CMT phenotype caused by a PMP22 duplication. Ann Neurol 2005;57:589–591
Multiple regions of α‐synuclein are associated with Parkinson's disease
Authors: Mueller Jakob C. , Fuchs Julia , Hofer Anne , Zimprich Alexander , Lichtner Peter , Illig Thomas , Berg Daniela , Wüllner Ullrich , Meitinger Thomas , Gasser Thomas 
Published: March 2005

Abstractα‐Synuclein is considered to play an important role in the pathogenesis of both the rare familial and the common sporadic forms of Parkinson's disease. Previous reports primarily have tested the association of α‐synuclein promoter polymorphisms with idiopathic Parkinson's disease, but results are controversial. We first characterized the linkage disequilibrium structure of the α‐synuclein gene region with a dense set of 56 genetic markers and subsequently performed two independent case–control association analyses using tagging markers. We could distinguish two large linkage disequilibrium blocks spanning the α‐synuclein gene. Several markers within the 3′‐block around exons 5 and 6 showed strong association with Parkinson's disease (p = 0.00009). Effects of the associated variants might be mediated by regulatory elements in this highly conserved region or by a frequency shift in a previously described splice variant lacking exon 5. A direct association with promoter polymorphisms could not be replicated in our sample set. A second set of markers in the 5′‐block of the gene were also significantly associated with Parkinson's disease, when young patients and female subjects were analyzed separately. These findings indicate locus heterogeneity for the pathogenesis of Parkinson's disease in different genetic or physiological environments, related to sex and age. Ann Neurol 2005;57:535–541
Murine gammaherpesvirus‐68 infection of mice: A new model for human cerebral Epstein–Barr virus infection
Authors: Häusler Martin , Sellhaus Bernd , Scheithauer Simone , Engler Matthias , Alberg Evelyn , Teubner Andreas , Ritter Klaus , Kleines Michael 
Published: March 2005

AbstractEpstein–Barr virus infection may cause severe neurological complications that are not mirrored by animal models. Here, we show that nasal inoculation of newborn BALB/c wild‐type mice with MHV‐68, a murine gammaherpesvirus, causes cerebral infection with inflammation in 50% of the animals. The inflammatory patterns are strikingly similar to those known from Epstein–Barr virus, including hydrocephalus, meningitis, cerebellitis, focal or diffuse encephalitis, and temporal lobe encephalitis. This offers a new powerful tool to study the virological and immunological characteristics of cerebral gammaherpesvirus infections. Ann Neurol 2005;57:600–603
Commonality of TRIM32 mutation in causing sarcotubular myopathy and LGMD2H
Authors: Schoser Benedikt G. H. , Frosk Patrick , Engel Andrew G. , Klutzny Ursula , Lochmüller Hanns , Wrogemann Klaus 
Published: March 2005

AbstractSarcotubular myopathy (OMIM 268950) is a rare autosomal recessive myopathy first described in two Hutterite brothers from South Dakota and in two non‐Hutterite brothers from Germany. We report that sarcotubular myopathy (STM) is caused by mutation in TRIM32, the gene encoding the tripartite motif‐containing protein 32. TRIM32 was found to be the gene mutated in limb girdle muscular dystrophy type 2H (LGMD2H [OMIM 254110]), a disorder that has been confined to the Hutterite population. The TRIM32 mutation found in the STM patients is identical to the causative mutation for LGMD2H (D487N), Haplotype analysis shows that the disease chromosomes share common ancestry. Ann Neurol 2005;57:591–595
The metabolic pathology of dopa‐responsive dystonia
Authors: Asanuma Kotaro , Ma Yilong , Huang Chaorui , Carbon‐Correll Maren , Edwards Christine , Raymond Deborah , Bressman Susan B. , Moeller James R. , Eidelberg David 
Published: March 2005

AbstractWe used [18F]‐fluorodeoxyglucose and positron emission tomography to determine a discrete cerebral pattern of abnormal glucose utilization in dopa‐responsive dystonia. Network analysis demonstrated that dopa‐responsive dystonia is associated with a specific pattern of regional metabolic covariation, characterized by increases in the dorsal midbrain, cerebellum, and supplementary motor area, as well as reductions in motor and lateral premotor cortex and in the basal ganglia. This pattern was not expressed in mutation carriers for primary torsion dystonia. Dopa‐responsive dystonia has a unique metabolic architecture that differs from other inherited forms of dystonia. Ann Neurol 2005;57:596–600
Convection perfusion of glucocerebrosidase for neuronopathic Gaucher's disease
Authors: Lonser Russell R. , Walbridge Stuart , Murray Gary J. , Aizenberg Michele R. , Vortmeyer Alexander O. , Aerts Johannes M. F. G. , Brady Roscoe O. , Oldfield Edward H. 
Published: March 2005

AbstractSystemic enzyme replacement for Gaucher's disease has not prevented premature death or severe morbidity in patients with a neuronopathic phenotype, because the enzyme does not cross the blood–brain barrier. We used convection‐enhanced delivery for regional distribution of glucocerebrosidase in rat and primate brains and examined its safety and feasibility for neuronopathic Gaucher's disease. Rats underwent intrastriatal infusion and were observed and then sacrificed at 14 hours, 4 days, or 6 weeks. Primates underwent serial magnetic resonance imaging during enzyme perfusion of the right frontal lobe or brainstem, were observed and then sacrificed after infusion completion. Animals underwent histologic and enzymatic tissue analyses. Magnetic resonance imaging revealed perfusion of the primate right frontal lobe or pons with infusate. Enzyme activity was substantially and significantly (p < 0.05) increased in cortex and white matter of the infused frontal lobe and pons compared to control. Immunohistochemistry demonstrated intraneuronal glucocerebrosidase. There was no toxicity. Convection‐enhanced delivery can be used to safely perfuse large regions of the brain and brainstem with therapeutic levels of glucocerebrosidase. Patients with neuronopathic Gaucher's disease and similar central nervous system disorders may benefit from this treatment. Ann Neurol 2005;57:542–548
Die Jagd auf Moleküle, die nie existiert haben: Falsch zugeordnete Naturstoffstrukturen und die Rolle der chemischen Synthese in der modernen Strukturaufklärung
Authors: Nicolaou K. C. , Snyder Scott A. 
Published: March 2005

AbstractSiehe Originalbeitrag Angew. Chem. 2005, 117, 1036–1069
Macroporous SiCMoSi2ceramics from templated hybrid MoCl5–polymethylsilane
Authors: Wang Hao , Li Xiao‐dong , Kim Dong‐pyo 
Published: March 2005

AbstractA molybdenum‐containing preceramic polymer, MoPMS, was synthesized for the first time by HCl elimination of polymethylsilane (PMS) and MoCl5at room temperature in tetrahydrofuran. The insoluble MoPMS prepared was embedded into the void spaces of a silica colloidal crystal template within the pot life of the polymer and successfully transformed to a three‐dimensionally long‐range‐ordered macroporous SiCMoSi2ceramic after pyrolysis at 1400 °C in an argon atmosphere followed by template removal in HF. The bead‐inverse macroporous SiCMoSi2ceramic, with a ceramic yield of about 88%, exhibits high temperature stability, high BET surface area, and semiconducting behavior. In addition, the macroporous SiCMoSi2ceramic was used as a catalyst carrier for platinum–ruthenium coated on the surface of the pores. The preceramic polymer and the ceramic were characterized by IR, thermogravimetric analysis, X‐ray diffraction, scanning and transmission electron microscopy, and BET surface area. Copyright © 2005 John Wiley & Sons, Ltd.
Structural and physicochemical characterization of zinc(II) and cadmium(II) complexes with 1,4‐dimethy‐lhomopiperazine
Authors: Matsunaga Yuki , Fujisawa Kiyoshi , Amir Nagina , Miyashita Yoshitaro , Okamoto Ken‐ichi 
Published: March 2005

AbstractIn order to know the relationship between structures and physicochemical properties of Group 12 metal(II) ions, the complexes with ‘simple’ ligands, such as alkyl cyclic diamine ligand and halide ions, were synthesized by the reaction of 1,4‐dimethylhomopiperazine (hp′) with MX2 as metal sources (M = Zn, Cd; X = Cl, Br, I). The five structural types, [ZnX2(hp′)] (X = Cl (1), Br (2) and I (3)), [ZnX3(Hhp′)] (X = Cl (1′) and Br (2′)), [CdCl2(hp′)]n (4), [{CdCl2(Hhp′)}2(µ‐Cl)2] (4′) and [{CdX(hp′)}2(µ‐X)2] (X = Br (5), I (6)), were determined by X‐ray analysis. The sizes of both metal(II) and halide ions and the difference in each other's polarizability influence each structure. All complexes were characterized by IR, far‐IR, Raman and UV–Vis absorption spectroscopies. In the far‐IR and Raman spectra, the typical ν(MN) and ν(MX) peaks clearly depend on the five structural types around 540–410 cm−1 and 350–160 cm−1 respectively. The UV–Vis absorption band energy around 204–250 nm also reflects each structural type. Copyright © 2005 John Wiley & Sons, Ltd.
Air stable Fe and FeCo magnetic fluids—synthesis and characterization
Authors: Bönnemann H. , Brand R. A. , Brijoux W. , Hofstadt H.‐W. , Frerichs M. , Kempter V. , Maus‐Friedrichs W. , Matoussevitch N. , Nagabhushana K. S. , Voigts F. , Caps V. 
Published: March 2005

AbstractThermolysis of Fe(CO)5 and Co2(CO)8, dissolved in tetrahydronaphthalene, in the presence of aluminum trialkyl leads to uniform‐sized Fe or FeCo nanoparticles, respectively. Subsequent treatment with very dilute oxygen forms a shell which protects the metallic or alloyed core of the particles against further oxidation. With the help of surfactants, for instance oleic acid or cashew nut shell liquid, the particles can be peptized in organic solvents like toluene or kerosene, resulting in magnetic fluids with extraordinary magnetic properties. The saturation of magnetization, Ms, of the fluids was determined by specific magnetization. The sizes and structure of the particles were investigated by transmission electron microscopy, and Moessbauer analysis showed that the core of the particles was metallic or alloyed, respectively. The particle surface termination was studied by X‐ray photoelectron spectroscopy and Auger electron spectroscopy. Copyright © 2005 John Wiley & Sons, Ltd.
Aluminum alkyls with intramolecularly coordinated oxygen
Authors: Dostál Libor , Jambor Roman , Ru̇žička Aleš , Císařová Ivana , Holeček Jaroslav 
Published: March 2005

AbstractOrganoaluminum alkyls L1AlMe2 (1), L2AlMe2 (2) and L2AliBu2 (3) with O,C,O‐chelating ligands L1 and L2 [L1 = 2, 6‐(MeOCH2)2C6H3 and L2 = 2, 6‐(tBuOCH2)2C6H3] were prepared. The compounds have been characterized by elemental analysis, 1H, 13C, 27Al NMR spectroscopy and X‐ray diffraction analysis (3). Solution NMR studies indicated the four coordinated aluminum atom and dissociation/association dynamic process in solution of 1–3. The X‐ray diffraction analysis of 3 showed that the aluminum atom is [4 + 1] coordinated with the trans‐trigonal bipyramidal geometry. The reactivity of 2 was investigated. Reactions of 2 with MeOH and I2 resulted in aluminum alkoxide [L2Al(OMe)2 (4)] and iodides [L2AlI2· THF (5) and L2AlI2 (6)], respectively, characterized by elemental analysis, IR and 1H, 13C and 27Al NMR spectroscopy. Copyright © 2005 John Wiley & Sons, Ltd.
Developmental abnormalities in rat embryos leading to tibial ray deficiencies induced by busulfan
Authors: Otsuji Miwako , Takahara Masatoshi , Naruse Takuji , Guan Dehong , Harada Mikio , Zhe Piao , Takagi Michiaki , Ogino Toshihiko 
Published: March 2005

AbstractBACKGROUNDLittle is known about the developmental changes associated with tibial ray deficiencies. The aim of this study was to detect cell death, proliferation, and gene expression that result in tibial ray deficiencies.METHODSWe induced tibial ray deficiencies in rat embryos using a teratogenic agent (busulfan) and observed the developmental changes in 1126 hindlimbs. We performed Nile blue staining, whole mount in situ hybridization for fibroblast growth factor 8 (Fgf8), bone morphogenetic protein 4 (Bmp4) and Sonic hedgehog (Shh), terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end‐labeling (TUNEL) and assessment of cell proliferation by 5‐bromo‐2′‐deoxy‐uridine (BrdU)/anti‐BrdU immunohistochemistry.RESULTSIn situ hybridization showed reductions in Fgf8 and Bmp4 expression. Histological examination showed a delay of mesenchymal condensation, increased mesenchymal cell death, decreased mesenchymal cell proliferation, and a reduction in the number of mesenchymal cells. These abnormalities may cause hypoplasia of the limb. Bmp4 expression was markedly reduced in the anterior mesenchyme. Shh was expressed in the posterior mesenchyme. We suggest that the posterior skeletal elements may be fully formed owing to Shh expression, but the anterior skeletal elements may be underdeveloped owing to an intense reduction of Bmp4 expression in the anterior mesenchyme, causing hypoplasia of the tibial ray.CONCLUSIONSThe combined effects of increased cell death, decreased cell proliferation, reduction of Fgf8 expression, and intense reduction of Bmp4 expression in the anterior mesenchyme may play an important role in the development of tibial ray deficiency induced by busulfan. Birth Defects Research (Part A), 2005. © 2005 Wiley‐Liss, Inc.
Comparative study of SP[6–11] and its analogs using simulated annealing
Authors: Bogár Ferenc , Leitgeb Balázs , Paragi Gábor , Orosz Antal , Penke Botond 
Published: March 2005

AbstractIn this study we compared the steric structures of the bioactive part of substance P (SP[6–11]) and its analogs (NY3460 and pHOPA‐SP5). The molecular dynamics–simulated annealing method was used to explore the conformational space, and the structural differences and similarities of these molecules were identified. For the three peptides, the conformational distributions were represented in Ramachandran density plots. The occurring secondary structural elements of the investigated molecules were identified, namely α‐Helix, type III β‐Turn, γ‐Turn, and inverse γ‐Turn. For SP[6–11] and its two analogs, different intramolecular interactions (H‐bonds between the main‐chain atoms, aromatic–aromatic interactions, and amino–aromatic interactions) that can stabilize the various conformations of the three peptides were investigated. Detailed examination of these intramolecular interactions revealed that H‐bonds between the main‐chain atoms are relevant in the determination and stabilization of the conformer structures of the peptides, while the aromatic–aromatic interactions do not play an important stabilizing role. Furthermore, in the conformers of NY3460 and pHOPA‐SP5, different types of amino–aromatic interactions were identified that contribute to the formation of the various structures of these peptides. For all three molecules, the orientations of the side chains were investigated and the rotamer populations were determined. © 2005 Wiley Periodicals, Inc. Biopolymers, 2005
Laparoscopic restorative proctocolectomy (Br J Surg 2005; 92: 88–93)
Authors: Bemelman W A , D'Hoore A 
Published: March 2005

Abstract
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Volatile Organic Compounds from Arctic Bacteria of the Cytophaga‐Flavobacterium‐Bacteroides Group: A Retrobiosynthetic Approach in Chemotaxonomic Investigations
Authors: Dickschat Jeroen S. , Helmke Elisabeth , Schulz Stefan 
Published: March 2005

AbstractVolatile organic compounds emitted by different marine arctic strains of the Cytophaga‐Flavobacterium‐Bacteroides group were investigated by using a modified closed‐loop stripping apparatus (CLSA). Seven of nine strains emitted volatiles, dominated by methyl ketones, in specific patterns. The methyl ketones were aliphatic saturated, or unsaturated, and comprised 12 to 18 C‐atoms, sometimes with terminal Me branches. They were identified by GC/MS, retention‐index calculations, derivatization with dimethyl disulfide for CC bond location, and GC/FTIR to elucidate their uniform (Z)‐configuration. The proposed structures of all methyl ketones were subsequently confirmed by synthesis, while the absolute configuration of chiral volatiles was elucidated by stereoselective synthesis. From retrobiosynthetic considerations, it was found that strain ARK10267 uses mainly valine, and strain ARK10063 mainly isoleucine for formation of starters for the ketone biosynthesis, which is correlated to fatty acid biosynthesis. Four strains (ARK10223, ARK10044, ARK10141, and ARK10146) use leucine. These separations are supported by phylogenetic affiliations based on 16S rRNA. Strain ARK10255b, in the course of this study found to be not a member of the Cytophaga‐Flavobacterium‐Bacteroides phylum, did not emit aliphatic ketones of medium chain length, but methionine‐derived 4‐(methylsulfanyl)butan‐2‐one and corresponding 4‐(methylsulfanyl)butan‐2‐ol. Most of the compounds described have not been reported previously from nature.
Antioxidant Quinonemethide Triterpenes from Salacia campestris
Authors: Carvalho Paulo R. F. , Silva Dulce H. S. , Bolzani Vanderlan S. , Furlan Maysa 
Published: March 2005

AbstractA new quinonemethide triterpene named as salacin, has been isolated from the root bark of Salacia campestris in addition to the known pristimerin, maytenin, 20α‐hydroxymaytenin, and netzahualcoyene. Salacin was identified on the basis of NMR‐spectral and mass spectrometric analysis. The free‐radical scavenging activities of the quinonemethide triterpenes salacin (1), pristimerin (2), maytenin (3), 20α‐hydroxymaytenin (4), and netzahualcoyene (5) towards DPPH have been evaluated and showed absorbance variation (ΔA) of 19, 20, 39, 28, 55, and 10%, respectively, having rutin (74% at 50 μM) and BHT (7% at 50 μM) as standard compounds.
Sorption of Lead, Cadmium, and Zinc on Sulfur‐Containing Chemically Modified Wastes of Fluted Pumpkin (Telfairia occidentalis Hook f.)
Authors: Horsfall Michael , Spiff Ayebaemi I. 
Published: March 2005

AbstractWe have tested the applicability of regular as well as sulfanylacetic acid (SA) modified fluted pumpkin waste biomass as adsorbents for Pb2+, Cd2+, and Zn2+ aqueous solutions by means of the batch‐sorption technique. The data revealed that SA modification produces a larger surface area, enhancing the metal‐ion binding capacity of the biomass. The sorption process was examined by means of Freundlich and Langmuir models. The kinetic study showed that the sorption rates can be described by a pseudo‐second‐order process. The rate constants for the control biomass (CB) were 2.2×10−2, 4.4×10−2, and 1.6×10−2 mg g−1 min−1 for Pb2+, Cd2+, and Zn2+, respectively; and the corresponding rate constants for the SA‐modified biomass were 4.0×10−2, 4.7×10−2, and 1.7×10−2 mg g−1 min−1, respectively. Thermodynamic considerations indicated a spontaneous exothermic process, which implies that physisorption is the main mechanism in the sorption process.
Spectrophotometric Determination with a Chromogenic Reagent of Lead in Vegetables
Authors: Kumar Kailasa Suresh , Krishnaiah Lingaladinne , Babu Sunnapu Hari , Suvardhan Kanchi , Swaroop Lokanath B. , Chiranjeevi Pattium 
Published: March 2005

AbstractA method was developed for the simple, selective, and sensitive spectrophotometric determination of lead in vegetables with synthesized chromogenic reagent 3‐[(2,6‐dibromo‐4‐methylphenyl)diazenyl]‐4,5‐dihydroxy‐6‐[(2,4,6‐tribromophenyl)diazenyl]naphthalene‐2,7‐disulfonic acid (DBMTBA; 1). In 0.25M phosphoric acid medium, which greatly increases the selectivity, lead reacts with DBMTBA to form a 1 : 2 blue complex, which shows maximum absorption at 646 nm. Under optimal conditions, Beer's law is obeyed over the range from 0.09 to 0.8 μg ml−1 Pb2+, and the apparent molar absorptivity is 1.024×105 l mol−1 cm−1. The detection limit and the variation coefficient were found to be 2.09 μg ml−1 and 1.0%, resp. The proposed method has been applied successfully for the determination of lead in vegetables (Solanum melongena fruits, tomato fruits, Ablemoschus esculentus leaves, and Daucous carota leaves) with satisfactory results.
Microbial Transformation of Mesterolone
Authors: Choudhary M. Iqbal , Sultan Sadia , Jalil Saima , Anjum Shazia , Rahman Azhar Abdul , Fun Hoong‐Kun , Atta‐ur‐Rahman  
Published: March 2005

AbstractThe microbial transformation of mesterolone (=(1α,5α,17β)‐17‐hydroxy‐1‐methylandrostan‐3‐one; 1), by a number of fungi yielded (1α,5α)‐1‐methylandrostane‐3,17‐dione (2), (1α,3β,5α,17β)‐1‐methylandrostane‐3,17‐diol (3), (5α)‐1‐methylandrost‐1‐ene‐3,17‐dione (4), (1α,5α,15α)‐15‐hydroxy‐1‐methylandrostane‐3,17‐dione (5), (1α,5α,6α,17β)‐6,17‐dihydroxy‐1‐methylandrostan‐3‐one (6), (1α,5α,7α,17β)‐7,17‐dihydroxy‐1‐methylandrostan‐3‐one (7), (1α,5α,11α,17β)‐11,17‐dihydroxy‐1‐methylandrostan‐3‐one (8), (1α,5α,15α, 17β)15,17‐dihydroxy‐1‐methylandrostan‐3‐one (9), and (5α,15α,17β)‐15,17‐dihydroxy‐1‐methylandrost‐1‐en‐3‐one (10). Metabolites 5–10 were found to be new compounds. All metabolites, except 2, 3, 6, and 7, exhibited potent anti‐inflammatory activity. The structures of these metabolites were characterized on the basis of spectroscopic studies, and the structure of 5 was also determined by single‐crystal X‐ray‐diffraction analysis.