bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-33 signaling is dispensable for the IL-10-induced enhancement of mast cell responses during food allergy
Authors: Krajewski Dylan , Ranjitkar Saurav , Jordan Nathan , Schneider Sallie S. , Mathias Clinton B. 
Published: January 2025

BackgroundThe IL-33/ST2 axis plays a pivotal role in the development of IgE-mediated mast cell (MC) responses during food allergy. We recently demonstrated that the pleiotropic cytokine, IL-10, not only exerts proinflammatory effects on IgE-mediated MC activation, but also promotes IL-33-induced MC responses. However, whether IL-33 is necessary for IL-10’s proinflammatory effects has not been examined.MethodsTo therefore determine the role of the IL-33/ST2 axis in this pathway, we assessed the effects of IL-10 on IgE-mediated MC activation and food allergy development in wild-type (WT) and ST2-/- mice.ResultsIL-10 stimulation significantly enhanced IL-33 gene expression, ST2 receptor expression, cytokine production, mMCP-1 secretion, and proliferation in IgE and antigen-activated bone marrow-derived MCs (BMMCs) from WT mice. ST2-/- BMMCs exhibited reduced cytokine secretion in response to IgE-dependent activation. However, IL-10 enhanced cytokine production, mMCP-1 secretion, and proliferation in these cells as well. To further assess the role of IL-10, food allergy was induced in WT and ST2-/- mice subjected to antibody-mediated IL-10 depletion. IL-10-depleted WT mice exhibited a significant attenuation in MC-mediated responses to OVA challenge. While ST2-/- mice also exhibited a profound suppression of MC responses, IL-10 depletion had no additional effects. However, ST2-/-/IL-10-/- mice exhibited further decreases in OVA-IgE and antigen-specific MC activation compared to ST2-/- mice.ConclusionOur data demonstrates that IL-10 can enhance MC responses in both WT and ST2-/- mice, further corroborating its proinflammatory effects on MCs and suggesting that they are not regulated by IL-33 signaling.
Bug off or bug out: mapping flight secrets of Triatoma garciabesi (Hemiptera: Reduviidae) through climate, geography, and greenery
Authors: Verly Thaiane , Fiad Federico G. , Carbajal-de-la-Fuente Ana Laura , Pita Sebastián , Piccinali Romina V. , Lobbia Patricia A. , Sánchez-Casaccia Paz , Rojas de Arias Antonieta , Cavallo María José , Gigena Gisel V. , Rodríguez Claudia S. , Vega-Gómez María C. , Rolón Miriam , Nattero Julieta 
Published: January 2025

IntroductionTriatoma garciabesi is a vector of Trypanosoma cruzi, the causative agent of Chagas disease, and is found across northwest and central Argentina, southern Bolivia, and western Paraguay. It frequently invades rural houses during the warm seasons and is common in peridomestic and wild environments. Recently, the existence of two lineages has been demonstrated based on variation in cytochrome c oxidase I gene (coI). These lineages occur across the species distribution range and coincide with different ecological regions. Here, we aim to examine how phenotypic variation in flight-related traits is structured, determine the association between these traits and geographic distance, and identify the climatic, geographic, and/or vegetation cover variables that best explain the morphometric variation in flight-related traits.MethodsA total of 198 males of T. garciabesi from 24 populations in Argentina and Paraguay were included in this study, covering almost the entire T. garciabesi distribution range. Size and shape components of the forewing, membranous, and stiff portions of the forewing and head were measured using a landmark-based methodology.ResultsOur study documents that the size component of the membranous and stiff portions showed significant variation across the species range. Although forewing and head shape did not show significant differences in Procrustes distances across all pairs of populations, the membranous and stiff portions did. There is a strong and consistent association between shape and geographic distances at all levels of comparison (species and lineage ranges). The size and shape components and the geographic, climatic, and/or vegetation indexes explained covariation in all flight-related traits.DiscussionT. garciabesi appears to be a species sensitive to vegetation cover and landscape features. This study provides evidence for this by showing clear variation in flight-related traits across the species and lineage distribution range, as well as indications of isolation by distance and variation in flight-related traits according to climate, geography, and vegetation cover.
Validation of wing geometric morphometrics in Chrysodeixis spp. (Lepidoptera: Noctuidae) to support pest identification in invasive species survey programs
Authors: Smith-Pardo Allan H. , Torres Karina M. , Paula-Moraes Silvana V. 
Published: January 2025

Looper moths of the genus Chrysodeixis (Lepidoptera: Noctuidae: Plusiinae) are important pests of many crops and native plants worldwide. Chrysodeixis chalcites (Esper) is listed as an invasive species for the United States with records of interception. Native species of the Plusiinae subfamily are morphologically similar and commonly cross-attracted in survey trapping programs for C. chalcites, such as Chrysodeixis includens (Walker), a native economic pest. The species identification relies on male genitalia dissection and DNA analysis. These processes are time and cost-consuming and require expertise. In this work, we evaluated the use of wing geometric morphometrics (GM) as a tool to overcome the identification challenges associated with the complex morphologies of Chrysodeixis spp. The cleaned wings of specimens with validated identification were photographed under a digital microscope, and seven venation landmarks were annotated from the images. The digital coordinates of the wing landmarks were analyzed in MorphoJ. Our results validated the use of GM for distinguishing the invasive C. chalcites from the native C. includens. A limited number of landmarks on the center of the wing was used to address the challenges in GM for trap-collected lepidopteran pests. Future automation of the novel application of GM for identifying C. includens can be explored in trapping systems for IPM and surveys for the invasive C. chalcites.
Parasitological and clinical correlates of Plasmodium–Schistosoma mansoni co-infection and its determinants among Plasmodium-infected patients in the Wolaita Zone, South Ethiopia regional state
Authors: Mohamed Kemal , Belay Tariku , Zeynudin Ahmed 
Published: January 2025

BackgroundMalaria and schistosomiasis are among the most important parasitic diseases with overlapping geographical distribution in Ethiopia. The objective of this study, therefore, was to assess parasitological and clinical correlates and determinants of co-infection among malaria patients.MethodsA health facility-based comparative cross-sectional study was conducted among malaria patients attending public health facilities in Damot Woyide district, Wolaita zone, South Ethiopia regional state, from December 2020 to June 2021. A total of 246 (123 Plasmodium-only and 123 Plasmodium–Schistosoma mansoni co-infected) study participants were sampled consecutively. A pre-tested structured questionnaire was used to collect data on demographic and risk factors. Detection and quantification of Schistosoma mansoni ova and malaria parasites were done with the Kato–Katz technique and blood film, respectively. Determination of hematological and biochemical parameters was done by the aLCose®HemoGo test system and the Cobas C 311 chemistry analyzer, respectively.ResultsThe prevalence of Plasmodium–Schistosoma mansoni co-infection was 18.2%. Plasmodium–Schistosoma mansoni co-infected with a heavy intensity of Schistosoma mansoni had the highest parasitemia and was also significantly associated with malaria attack. In addition, anemia was associated with the presence of Plasmodium–Schistosoma mansoni co-infection. Schistosoma mansoni co-infection among malaria patients significantly increased mean Plasmodium density, serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, direct bilirubin, and total protein. Hemoglobin and hematocrit were significantly lower among co-infected individuals. On multivariate analysis, being male (AOR=3.03, CI: 1.56-5.91,p=0.001), being in the 6-15 years age group (AOR = 5.85, CI: 2.45–13.93,p=0.00), the presence of stream (AOR = 4.1, CI: 1.65–10.15,p=0.002), having a water body distance of less than 1,000 m (AOR=2.33, CI: 1.23–4.41,p=0.009), and presence of irrigation practice (AOR= 3.24, CI: 1.69–6.18,p=0.00) were found to be significant risk factors of co-infection.ConclusionMalaria attack and level of parasitemia were associated with the intensity of concurrent Schistosoma mansoni infection. Co-infection results in the change of Plasmodium density, hematological, and biochemical parameters in malaria patients.
Managing two cases of twin anemia–polycythemia sequence in monochorionic twin pregnancies
Authors: Zhang Yongke , Liang Yiheng , Chen Ran , Zhang Dirong , Wu Jing 
Published: January 2025

BackgroundTwin anemia–polycythemia sequence (TAPS) is a rare monochorionic complication. In this study, we discuss the management of two cases of TAPS with different conditions.Case presentationPatient 1 was a 34-year-old multigravida in whom the fetal middle cerebral artery peak systolic velocities (MCA-PSVs) were 0.86 multiples of the median (MoM) and 2.0 MoM at 33 weeks of gestation. After cesarean section, stage 3 TAPS was confirmed according to the ultrasound findings and hemoglobin results of the newborns and placenta examination after birth. Patient 2 was a nulligravida who was diagnosed with stage 2 TAPS at 18 weeks of gestation. The patient underwent fetoscopic laser surgery. Ultrasonography monitoring of the MCA-PSVs was performed on a schedule after surgery, with a good status but selective intrauterine growth restriction of one cotwin. The newborns reached their normal development milestones after spontaneous preterm birth.ConclusionOptimal management should be carefully selected for patients with different TAPS conditions.
Prevalence and clinical profile of comorbidity among newly diagnosed pulmonary tuberculosis patients: a multi-center observational study in eastern China
Authors: Wang Wei , Wang Xiaomeng , Chen Songhua , Li Jun , Cheng Qinglin , Zhang Yu , Wu Qian , Liu Kui , Jiang Xuli , Chen Bin 
Published: January 2025

ObjectiveTo identify the composition of comorbidities among patients with newly diagnosed pulmonary tuberculosis and assess the impact of comorbidities on the clinical characteristics of patients.MethodsThis study was conducted in 13 hospitals across 13 counties in Zhejiang province, China. Patient data collected in this study included demographic characteristics, chest radiography results, etiological results, and comorbidities. Descriptive statistics were conducted to describe the composition of comorbidities of all participants. Univariate and multivariate logistic regression analyzes were performed to identify the effects of comorbidities on the clinical features of the participants.ResultsOf the 8,421 total participants, 27.6% reported cavities in the chest radiography results, 41.9% were Mycobacterium tuberculosis-positive in the etiology test results, and 38.7% (3,258/8,421) had at least one type of comorbidity. The most predominant comorbidity was pleuritis (1,833, 21.8%), followed by diabetes mellitus (763, 9.1%), other extrapulmonary tuberculosis (421, 5%), tracheobronchial tuberculosis (275, 3.3%), and silicosis (160, 1.9%). Participants with diabetes mellitus had the highest rate of chest cavities on X-ray (54.8%), followed by those with silicosis (33.1%). In addition, a higher percentage of the M. tuberculosis-positive etiology (45%) was observed in participants without comorbidities than in participants with comorbidities (37.1%). Compared to patients without comorbidities, patients with diabetes mellitus (adjusted odds ratio [AOR]: 2.88, 95% confidence interval [CI]: 2.42–3.43) were more likely to show cavities in chest X-ray, while patients with pleuritis (AOR: 0.27, 95% CI: 0.23–0.32), other extrapulmonary tuberculosis (AOR: 0.48, 95% CI: 0.36–0.64), and tracheobronchial tuberculosis (AOR: 0.40–0.79) were less likely to show chest cavities in X-ray. In addition, patients with diabetes mellitus (AOR: 2.05, 95% CI: 1.72–2.45), tracheobronchial tuberculosis (AOR: 3.22, 95% CI: 2.4–4.32) were more likely to show Mycobacterium tuberculosis-positive in the etiology, and patients with pleuritis (AOR: 0.25, 95% CI: 0.22–0.29), other extrapulmonary tuberculosis (AOR: 0.61, 95% CI: 0.48–0.76) were less likely to show Mycobacterium tuberculosis-positive in the etiology.ConclusionThe prevalence of comorbidities was high in patients newly diagnosed with pulmonary tuberculosis. Thus, integration of screening and personalized management is needed for the control of tuberculosis and its comorbidities.
Global research trends on gastrointestinal cancer and mental health (2004–2024): a bibliographic study
Authors: Han Wenjin , Wang Tianmeng , He Zhiqiang , Wang Caihua , Hui Zhaozhao , Lei Shuangyan , Hao Nan , Li Ning , Wang Xiaoqin 
Published: January 2025

BackgroundGastrointestinal (GI) cancers impose a significant burden on global public health. Patients often experience mental health challenges due to physical changes and treatment-related symptoms, which can worsen their condition or delay recovery. Although research is mounting in this field, visual bibliometric analysis has not yet been conducted. This study aims to reveal the research hotspots and frontiers in this field using bibliometrics to guide future research.MethodsThe publications on GI cancer and mental health were retrieved in the Web of Science Core Collection from 2004 to 2024. VOS Viewer and CiteSpace, as commonly used bibliometric analysis tools, were employed to visualize the network structure of bibliometric data and uncover the evolving trends in scientific research fields. VOS Viewer was used to identify keyword co-occurrences, while CiteSpace was utilized to generate network visualizations, produce dual-map overlays of journals, and perform burst keyword analysis.ResultsA total of 1,118 publications were included for analysis. China had the highest number of publications in this field (341, 30.5%), while the United States held a central position (centrality = 0.48). The most productive author and institution were Floortje Mols and Tilburg University, respectively. Keyword analysis highlighted that “quality of life” (QoL) is a prominent research topic in the field, while “complications,” “cancer-related fatigue,” (CRF) “chronic stress,” and “epidemiology” have been identified as key areas for future research.ConclusionResearch interest in this field continues to grow. The research direction is mainly focused on personalized mental health interventions to improve QoL, as well as preoperative mental healthcare and ongoing care through internet-based multidisciplinary collaboration to reduce postoperative complications. More detailed clinical symptom assessment is needed to distinguish between CRF and mental health issues and to provide targeted intervention measures in the future. The mechanism of mental health effects on the occurrence and development of GI cancer will be a frontier.
Comparative analysis of the Streptococcus pneumoniae competence development in vitro versus in vivo during pneumonia-derived sepsis
Authors: Chong Sook Yin , Lew Shi Qian , Alam Tauqeer , Gaulke Christopher A. , Lau Gee W. 
Published: January 2025

IntroductionThe Streptococcus pneumoniae (pneumococcus) competence regulon is well-known for regulating genetic transformation but is also important for virulence. Some pneumococcal strains can enter a transient competent state for genetic transformation in an optimized competence-inducing medium when the threshold level of the peptide pheromone competence stimulating peptide is attained; upregulating the expression of three distinct phases of “early”, “late” and “delayed” competence genes. Recently, we discovered that pneumococcus can naturally enter a prolonged competent state during acute pneumonia in mice. However, mechanisms driving competence development during host infection are rarely examined, and a direct comparison between in vitro and in vivo competence induction has not been performed.MethodsWe conducted a comparative gene expression analysis of pneumococcal competence development in vitro versus in vivo during pneumonia-derived sepsis in mice. We examined existing RNA-Seq data and performed validation using RNA obtained from an independent replicate experiment.Results and discussionOur analysis revealed both similarities and differences in the expression of “early”, “late”, and “delayed” competence between in vitro versus during pneumonia-derived sepsis. Our results may reveal new aspects of pneumococcal competence biology.
Combining interictal intracranial EEG and fMRI to compute a dynamic resting-state index for surgical outcome validation
Authors: Boerwinkle Varina L. , Gunnarsdottir Kristin M. , Sussman Bethany L. , Wyckoff Sarah N. , Cediel Emilio G. , Robinson Belfin , Reuther William R. , Kodali Aryan , Sarma Sridevi V. 
Published: January 2025

IntroductionAccurate localization of the seizure onset zone (SOZ) is critical for successful epilepsy surgery but remains challenging with current techniques. We developed a novel seizure onset network characterization tool that combines dynamic biomarkers of resting-state intracranial stereoelectroencephalography (rs-iEEG) and resting-state functional magnetic resonance imaging (rs-fMRI), vetted against surgical outcomes. This approach aims to reduce reliance on capturing seizures during invasive monitoring to pinpoint the SOZ.MethodsWe computed the source-sink index (SSI) from rs-iEEG for all implanted regions and from rs-fMRI for regions identified as potential SOZs by noninvasive modalities. The SSI scores were evaluated in 17 pediatric drug-resistant epilepsy (DRE) patients (ages 3–15 years) by comparing outcomes classified as successful (Engel I or II) versus unsuccessful (Engel III or IV) at 1 year post-surgery.ResultsOf 30 reviewed patients, 17 met the inclusion criteria. The combined dynamic index (im-DNM) integrating rs-iEEG and rs-fMRI significantly differentiated good (Engel I–II) from poor (Engel III–IV) surgical outcomes, outperforming the predictive accuracy of individual biomarkers from either modality alone.ConclusionThe combined dynamic network model demonstrated superior predictive performance than standalone rs-fMRI or rs-iEEG indices.SignificanceBy leveraging interictal data from two complementary modalities, this combined approach has the potential to improve epilepsy surgical outcomes, increase surgical candidacy, and reduce the duration of invasive monitoring.
Virtual reality-based vestibular rehabilitation therapy in patients with acute unilateral vestibulopathy: a randomized controlled trial
Authors: Lee Jae Woo , Yoon Chul Young , Kim Jae Ha , Seo Young Joon , Kong Tae Hoon 
Published: January 2025

IntroductionConventional vestibular rehabilitation therapy (VRT) requires significant time and resources, especially for patients with low compliance, limiting its effectiveness and accurate assessment. Studies have shown VRT using virtual reality (VR) to be effective, with meta-analyses confirming its superiority over conventional methods. However, methodological variations in VR remain a limitation. This study aimed to assess the effects of VRT using head-mounted display (HMD) VR technology in patients with acute unilateral vestibulopathy (AUVP) and compare the outcomes with conventional VRT.MethodsWe conducted a single-blinded randomized controlled trial with 60 AUVP patients randomly assigned to VR or control groups. The VR group received VRT via VR, while the control group underwent conventional VRT. Both groups followed individual home-based programs for 8 weeks and visited the clinic every 2 weeks. Subjective dizziness symptoms were evaluated using the visual analog scale (VAS), dizziness handicap inventory (DHI), and Activities-specific Balance Confidence (ABC) scale. Compliance with home-based programs was assessed on a 0–2-point scale based on responses in a booklet guide.ResultsSeven patients were lost to follow-up, leaving 26 and 27 patients in the VR and control groups, respectively. The mean patient age was 56.91 ± 12.11 years; 22 men and 33 women were included. Two-way repeated measures analysis of variance showed significant improvement in both groups for DHI and ABC scores. However, changes in DHI, VAS, and compliance scores did not differ between groups. Improvement in the physical domain of the DHI and ABC scores was significantly faster in the VR group (p = 0.019 for DHI, p = 0.0020 for ABC).DiscussionVRT using VR technology showed comparable efficacy to conventional VRT in AUVP patients. The VR group demonstrated greater improvement in the physical domain of the DHI and ABC scales, indicating enhanced confidence in movement and reduced perception of physical handicap due to dizziness.
Clinical characteristics, diagnosis, treatment, and prognosis of Atezolizumab-induced encephalitis, aseptic meningitis or meningoencephalitis
Authors: Yan Qingzi , Hu Yixiang , Liu Xiang , Xia Hong 
Published: January 2025

ObjectiveImmune checkpoint inhibitors (ICIs) have revolutionized cancer treatment and expanded the range of tumor indications. However, as the usage of this medication has increased, related adverse events are increasingly being identified. Among these, Atezolizumab-induced encephalitis, aseptic meningitis, and meningoencephalitis remain poorly understood regarding clinical features. This study provides a comprehensive reference for classifying, identifying, and managing Atezolizumab-associated neurological adverse events, specifically encephalitis, aseptic meningitis, and meningoencephalitis.MethodsThis study systematically collected published case reports from public databases such as PubMed, Web of Science, Wanfang Database, and CNKI Database. Two independent reviewers screened titles and abstracts and then extracted data in the included article according to the designed table and analyzed the clinical characteristics of Atezolizumab-induced encephalitis, aseptic meningitis, or meningoencephalitis.ResultsA total of 17 articles were included, with 19 patients diagnosed with encephalitis, aseptic meningitis, or meningoencephalitis after Atezolizumab treatment. The most common presenting symptoms included fever, altered consciousness, fatigue, somnolence, and seizures. Diagnosis was primarily based on cerebrospinal fluid analysis, blood tests, and imaging studies, such as computed tomography (CT) scans. Treatment strategies typically involved systemic steroids, antiviral agents, antibiotics, and anti-epileptic medications, as appropriate.ConclusionNeurological immune-related adverse events may rapidly progress and impact prognosis. Therefore, clinical practitioners should have a deep understanding of these neurological immune-related adverse events, promptly diagnose them, and provide accurate and timely treatment.