bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dependency Tree-Integrator Method for Reducing Mislaid Data Errors in Water Demand Prediction
Authors: G Manikannan , K JAYANTHI 
Published: January 2025

Abstract

The water supply demand has grown significantly high par with the increase in population, activities, manufacturing, etc. in recent years. A wide range of research on water demand forecasts based on the accumulated statistical data is formulated to ensure sufficient recommendations on required water supply. Considering these facts, the problem of mislaid water data statistics impacting prediction accuracy is addressed here. This article proposes a Dependency Tree-Integrator Method (DTIM) using linear XGBoost learning to address the aforementioned issue. The proposed method generates two linear dependency trees based on the increasing order of water utilization and equivalent demand from the daily data collected. The presence of mislaid data breaks the linearity of the dependency tree, where classification is performed. This classification differentiates the demand and utilization to regularize the demand. The regularized inputs are integrated to forecast the difference in previous and current utilizations as demands. The dependency tree and the classification processes form the integrity of the linear XGBoost learning. The dependency tree fails if the linearity is interrupted by the mislaid data input that is identified as an error and the differentiation occurs. Through real-time data and statistical analysis, the proposed method’s efficacy is verified using accuracy, precision, mean error, and complexity metrics. Thus proposed DTI method improves prediction precision and accuracy by 11.23% and 11.87% under the different quarters considered.
Calcium oscillations in Mesenchymal Stem Cells, a control on cell cycle progression to influence cell fate towards proliferation or differentiation?
Authors: Vallet Leslie A , Marina SANCHEZ PETIDIER , Romain FERNANDES , Nataliia NAUMOVA , Caterina MERLA , Claudia CONSALES , Giorgia INNAMORATI , Andre Franck M , Mir Lluis M. 
Published: January 2025

Abstract

Background Mesenchymal stem cells (MSCs) have been shown to exhibit Ca2+ oscillations under regular culture conditions. Ca2+ oscillations are known to encode important information in their frequencies as well as in their amplitudes, and ultimately to control many cellular processes such as proliferation and differentiation. These Ca2+ oscillations are modified in cells differentiated from MSCs, especially in their frequencies. Understanding the regulation of the Ca2+ oscillations in MSCs, either during proliferation or differentiation, is a necessary step in the attractive perspective of controlling Ca2+ signaling to influence cell fate. Previous studies have shown that the application of a low amplitude direct current electric field, which decreased the frequency of Ca2+ oscillations in MSCs undergoing osteogenic differentiation, had an impact on the differentiation process.Methods Using fluorescent microscopy we evaluated the evolution of Ca2+ oscillations throughout the adipogenic and osteogenic differentiation processes. Then, using electrical stimulation with microsecond pulsed electric fields (µsPEFs), we manipulated the frequency of Ca2+ oscillations in MSCs and measured its consequences on cell growth.Results Although the evolution of the Ca2+ oscillation frequencies at the beginning of the differentiation processes was different between the adipogenic and osteogenic differentiations, we observed common features in the terminal stages of differentiation. The common features included a progressive decrease in the Ca2+ oscillation frequencies, before their complete arrest as the differentiations were arriving to their term. A relationship between the frequency of Ca2+ oscillations and the progression of the cell cycle, more precisely the G1 phase, could explain the frequencies of Ca2+ oscillations observed during proliferation and along the differentiations. We hypothesized that increasing the frequency of Ca2+ oscillations would promote proliferation, while decreasing it would promote differentiation under differentiating conditions. Using electrical stimulation with µsPEFs, we manipulated the frequency of Ca2+ oscillations in MSCs and their increase promoted cell proliferation.Conclusions The cell fate of MSCs, in particular cell growth, can be controlled by manipulating the frequency of Ca2+ oscillations. We propose hypotheses on the actors that could regulate the evolution of the Ca2+ oscillation frequency along the differentiation processes, based on data available in the literature.
IL-1 signaling enrichment in inflammatory skin disease loci with higher risk allele frequencies in African ancestry
Authors: Tsoi Lam , Dong Yumeng , Patrick Matthew , Sarkar Mrinal , Zhang Haihan , Bogle Rachael , Zhang Zhaolin , Dand Nick , Paulsen Michelle , Ljungman Mats , Betz Regina C. , Petukhova Lynn , Christiano Angela , Simpson Michael , Modlin Robert , Khanna Dinesh , Barker jonathan , Budunova Irina , Gharaee-Kermani Mehrnaz , Billi Allison , Elder James , Kahlenberg J. Michelle , Gudjonsson Johann 
Published: January 2025

Abstract

Inflammatory skin diseases (ISDs) exhibit varying prevalence across different ancestry background and geographical regions. Genetic research for complex ISDs has predominantly centered on European Ancestry (EurA) populations and genetic effects on immune cell responses but generally failed to consider contributions from other cell types in skin. Here, we utilized 273 genetic signals from seven different ISDs: acne, alopecia areata (AA), atopic dermatitis (AD), psoriasis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), and vitiligo, to demonstrate enriched IL1 signaling in keratinocytes, particularly in signals with higher risk allele frequencies in the African ancestry. Using a combination of ATAC-seq, Bru-seq, and promoter capture Hi-C, we revealed potential regulatory mechanisms of the acne locus on chromosome 2q13. We further demonstrated differential responses in keratinocytes upon IL1β stimulation, including the pro-inflammatory mediators CCL5, IL36G, and CXCL8. Taken together, our findings highlight IL1 signaling in epidermal keratinocytes as a contributor to ancestry-related differences in ISDs. CAPSULE SUMMARY: ISD-associated loci highlight IL-1 signaling enrichment in African ancestry. Multi-omics reveals regulatory mechanisms at 2q13 for acne.
Mycobacterium abscessus Urinary Tract Infection Associated with Urinary Catheter Use: A Case Report
Authors: Alanazi Majed , Alsaber Naif , Alharbi Nasser , Alissa Munirah , Alaskar Majed 
Published: January 2025

Abstract

Urinary tract infections (UTIs) are a common health issue, especially among the elderly. While most UTIs are caused by typical bacterial pathogens, infections with atypical organisms like Mycobacterium abscessus, a non-tuberculous mycobacterium (NTM), are exceedingly rare. This case report details one such case of a 78-year-old woman with a complex medical history who developed a persistent UTI due to M. abscessus associated with urinary catheter use. Despite multiple courses of antibiotics, her symptomatology persisted, leading to the isolation of M. abscessus via acid-fast bacilli (AFB) culture. This case makes evident the challenges associated with diagnosing and managing NTM UTIs, especially given the organism’s resistance to standard treatments and the patient’s multiple comorbidities. A comprehensive review of relevant literature is provided, further delineating the rarity of genitourinary M. abscessus infections and the need for specialized diagnostic and therapeutic approaches.
Spatiotemporal faunal connectivity across global seafloors
Authors: O'Hara Timothy , Hugall Andrew , Haines Margaret , Weber Alexandra Anh-Thu , Eichsteller Angelina , Brogger Martin , Eléaume Marc , Fujita Toshihiko , Kongsrud Jon , Arbizu Pedro Martinez , Mills V , Olbers Jennifer , Paulay Gustav , Ramil Fran , Samadi Sarah , Sands Chester , Sellanes javier , Solis-Marin Francisco , Moussalli Adnan 
Published: January 2025

Abstract

Our knowledge of biogeographic patterns and processes in the deep sea has been limited by the lack of integrated datasets that cover its vast extent. Here we analyse a new global dataset of genomic DNA sequences, spanning an entire taxonomic class of benthic invertebrates (Ophiuroidea), to obtain a broad understanding of phylogenetic divergence and biotic movement across all oceans, from coastal margins down to the abyssal plains. We show that regional faunas on the continental shelf are phylogenetically divergent, particularly at temperate and tropical latitudes. In contrast, assemblages in the deep sea are much more connected. Many temperate deep-sea lineages have achieved distribution ranges across the planet, including over the Quaternary period. A close relationship exists between deep-sea faunas of the North Atlantic and Southern Australia, on the opposite sides of the globe. Bathymetric interchange is not only reliant on vertical migration through isothermal polar waters but also occurs across the thermal depth gradients of tropical regions. The connected nature of deep-sea life should be an important consideration in marine conservation assessments.
Spillover effects from the divorce of peer parents: Evidence from student academic achievements in China
Authors: Chen Boou , Zhao Chunkai , Liu Xiaoyu 
Published: January 2025

Abstract

Although the impact of parental divorce on children’s human capital development has received sufficient attention from scholars, little has been paid to the potential spillovers. Based on a quasi-natural experiment with random student-classroom assignment in secondary schools in China, we aimed to explore whether the peer parental divorce (PPD) can have a negative spillover effect on students’ academic achievements in a class. The results show that the PPD negatively impacted students’ test scores and cognitive abilities, but this adverse effect is not reflected in non-cognitive abilities. Mechanism analyses suggested that this spillover effect may be explained by the decline in parental expectations, development of students’ negative expectations and learning behaviors, and drop in teachers’ efforts and enthusiasm. Moreover, we found that the negative spillovers of PPD are more pronounced for urban children, only children, and non-poor children. Furthermore, supports from teachers, parents, and friends is expected to mitigate this adverse spillover effects. Our study reveals the spillovers from parental divorce, suggesting that the negative effects of divorce on human capital are not confined within the family. These findings further provide some insights into mitigating the human capital loss from divorce.
The impact of population-wide lifestyle modifications on improving life expectancy in the Chinese population: a simulation study
Authors: Lv Jun , Sun Qiufen , Zhao Liyun , Yang Yuxiang , Ding Yinqi , Yu Canqing , Sun Dianjianyi , Pang Yuanjie , Pei Pei , Yang Ling , Chen Yiping , Du Huaidong , Du Ranran , Barnard Maxim , Chen Junshi , Chen Zhengming , Yu Dongmei , Li Liming 
Published: January 2025

Abstract

Background Despite considerable population-wide measures to promote the Chinese population’s healthy lifestyle, it is uncertain how much life expectancy (LE) would improve under current and greater policy targets on lifestyle behaviours.Methods We developed 5-year sex-specific all-cause mortality risk prediction models using data from the China Kadoorie Biobank (CKB). Based on nationally representative survey data from China Nutrition and Health Surveillance (2015), we simulated changes in the prevalence of four lifestyle factors: smoking status, alcohol consumption, total physical activity level, and dietary habits (daily intake amount of fresh fruits and fish/seafood). The LE for each hypothetical lifestyle intervention scenario was calculated based on the age-specific probability of death obtained from the risk prediction models.Results During a median follow-up of 12.1 years, the CKB documented 31,956 deaths in men and 24,593 deaths in women. The prediction models for men and women demonstrated good discrimination, with the overall area under the receiver-operating characteristic curve of 0.800 for men and 0.808 for women. In the ideal scenario of all lifestyle factors, that is, all current smokers quit smoking, excessive alcohol users reduced their alcohol intake to moderate levels, people under 65 and those aged 65 and older increased their physical activity level by one hour and half an hour per day of moderate-intensity physical activity, and all participants ate 200g more fresh fruits and 50g more fish/seafood per day, LE at age 30 would increase by 4.83 (95% confidence interval [CI]: 4.01, 5.82) and 5.39 (95% CI: 4.70, 6.18) years for men and women, respectively. In the practical scenario, where improvements in each of the lifestyle factors were approximately halved, the gains in LE at age 30 for both men and women could be half those of the ideal scenario.Conclusions Our findings reinforce the evidence that population-wide lifestyle modifications can improve the LE of the whole Chinese population. If the current policy targets set by the Healthy China Initiative could be raised, a greater increase in LE would be achieved.
High-elevation orogenic plateau influences ecosystems
Authors: Zhang Zhiyong , Wang Nan , Luffi Peter , Li Zhiheng , Yu Zhiqiang , Wan Bo , Zhu Jing-Jing , Xiang Dunfeng , Guo Chao , Xiao Wenjiao 
Published: January 2025

Abstract

The interactions between plate tectonic processes and ecosystems are well-documented, but the precise influence of tectonic evolution on ecosystems remains elusive. This study investigates Jurassic–Cretaceous Northeastern Asia, where the Yanliao and Jehol Biotas flourished amid a prominent tectonic transition from crustal shortening to extension. To explore the impact of these processes on the ecosystem, we use innovative whole-rock geochemical composition-elevation models, alongside a large dataset from Late Triassic–Early Cretaceous arc magmatic rocks, to estimate regional-scale variations in paleo-crustal thickness, paleo-elevation, and paleo-temperature. Our results suggest that during the Jurassic, Northeastern Asia experienced significant crustal thickening, elevation increasing, and inland-ward magmatism due to plate convergence. By the Early Cretaceous, magmatism migrated outboard, driven by Paleo-Pacific slab rollback, leading to differential crustal thickness and elevation across the region. We propose an orogenic plateau with elevations of 2.0–4.5 km and mean annual temperatures of -1.0–12.8°C, creating complex topography that impacted spatiotemporal evolution of regional ecosystems and explains the high-elevation and cold habitats of the Yanliao and Jehol Biotas.
Epilobium parviflorum, hemorrhagic risk with anticoagulants
Authors: Beghriche Imene , Bensalem Samia , Fendri Allaoua Hicham 
Published: January 2025

Abstract

Plants have a crucial importance in our ecosystem. They contain complex mixtures of compounds that can interact with various therapeutics. Among them, the small-flowered willowherb (Epilobium parviflorum) has seen its use expand in recent years, particularly for prostate diseases.
The aim of this work is to demonstrate the risk of interaction between the use of E. parviflorum and medications, especially anticoagulants, illustrated by the case of our patient suffering from dilated cardiomyopathy, treated with acenocoumarol 4 mg, a vitamin K antagonist (VKA), for over a year.
The recent intake of small-flowered willow herb tea, rich in tannins and flavonoids, increased the patient's INR (International Normalized Ratio), indicating an increased risk of haemorrhage. A therapeutic education session was then held, with management of interactions and monitoring of the patient until stabilisation.
The intake of E. parviflorum or any other plant with anticoagulants requires rigorous pharmacological therapeutic monitoring to anticipate the risk of bleeding and improve the quality of life of patients.
Detection of Colombian datura virus infecting Brugmansia × candida medicinal cultivars and evaluation of sap inoculation in Solanaceae plants
Authors: Oliveros-Garay Oscar Arturo , González-Almario Adriana 
Published: January 2025

Abstract

Angel’s trumpets (Brugmansia spp.) are important medicinal plants belonging to the Solanaceae family that were domesticated in the Andean region of South America. Indigenous communities from the Colombian Andes have created cultivars with unusual leaf shapes and different medicinal uses. These cultivars exhibit symptoms on leaves associated with viral infection, such as mosaic, vein chlorosis, and morphological deformities. Previous studies have shown that the most widespread virus in Brugmansia is the Colombian datura virus (CDV), a globally distributed potyvirus that also affects agriculturally significant hosts. The present study aimed to evaluate the health status related to viral infections in Colombian Brugmansia medicinal cultivars and the relationship between the presence of viruses and the expression of symptoms in their leaves. We searched for CDV in the transcriptomes from a diverse collection of Solanaceae species and found it mainly in Brugmansia medicinal cultivars and wild solanaceous species. Sap from leaves of B. × candida cultivars with different symptoms were used as a source of CDV inoculum to infect representative cultivated plants of the Solanaceae family. We confirmed CDV infection of mechanically inoculated plants by RT-PCR and sequencing. Furthermore, we confirmed CDV ability to cause leaf deformations in agriculturally important plants such as Physalis peruviana, Solanum lycopersicum, and for the first time, reported the symptoms of the infection in Solanum melongena. In conclusion, this study is pioneering in characterizing a virus involved in the domestication of a medicinal plant.
Intranasal monoclonal antibodies do not prevent respiratory infection in a randomized, controlled experimental infection trial
Authors: Terstappen Jonne , Nibbelke Elisabeth E. , Ascough Stephanie , Bergstrom Emma , Bont Louis J. , Buiteman Brigitte J.M. , Chiu Chris , Cianci Daniela , Dayananda Pete , Delemarre Eveline M. , de Groot-Mijnes Jolanda D.F. , McCall Matthew B.B. , Reitsma Johannes B. , Schuurman Rob , Taylor Lydia , Troelstra Annet , Versnel Anouk , Vlaskamp Jacqueline , Viveen Marco , Zoggel J.A.A. van , Mazur Natalie I. 
Published: January 2025

Abstract

Intramuscular monoclonal antibodies (mAbs) can prevent respiratory infections but are costly and not globally available. The development of intranasal mAbs offers a promising cost-reducing and non-invasive alternative to systemic administration, though clinical evidence is lacking. Intranasal palivizumab prevented respiratory syncytial virus (RSV) infection in mice but not in healthy infants. This proof-of-concept study evaluates efficacy of intranasal palivizumab to prevent RSV infection. In this randomized, double-blind, placebo-controlled, experimental infection model, 28 healthy adults (18-55 years old) were randomized 1:1 to receive 1 mg/mL palivizumab in saline nasal drops or placebo. All participants were inoculated intranasally with RSV-A Memphis 37b strain one hour after administration of intranasal prophylaxis. The primary outcome of viral load was determined by RT-qPCR of nasal-wash samples 2-14 days post-inoculation. Symptoms were recorded by self-completed diaries. Intranasal palivizumab had no effect on viral load despite an 80% reduction in symptomatic infections. Upper respiratory tract symptoms over time were marginally lower in the intervention group compared to placebo. Intranasal palivizumab does not protect against RSV infection under optimally controlled conditions. Despite promising results in animal models, intranasal mAbs are highly unlikely to be a feasible and affordable method of protection against respiratory infection without high and frequent dosing. Funding: Lung Foundation Netherlands Junior Investigator Grant 5.2.20.020
Exploring the Physicochemical Compatibility of Minoxidil in Combination with Different Active Pharmaceutical Ingredients in Ready-to-use Vehicles for Alopecia Treatment
Authors: Marianni Bruna , Koulouridas Savvas , Polonini Hudson Caetano 
Published: January 2025

Background:
Alopecia is globally known as a distressing medical disorder that affects
men and women, and current commercially available minoxidil solutions are formulated with irritant
vehicles with frequent complaints of dermatologic adverse effects.

Objectives:
This study aimed to investigate further the compatibility of ready-to-use vehicles for the
preparation of tailored formulations for alopecia treatment, namely TrichoSol™ (a ready-to-use vehicle
for personalized hair solutions) and TrichoFoam™ (a ready-to-use vehicle for personalized
foam formulations), in combination with minoxidil and other active pharmaceutical ingredients
(APIs), to establish adequate beyond-use dates (BUD) for the given formulations.

Methods:
Products under evaluation were compounded using TrichoSol™ or TrichoFoam™, with
direct incorporation of the APIs into these vehicles. Samples were then stored at controlled room
temperature for up to 180 days. High-performance liquid chromatography (HPLC) methods were
developed and validated, and then utilized to evaluate the compatibility of the APIs in TrichoSol™
and TrichoFoam™. Forced degradation studies were conducted to assess API stability under various
stress conditions, and Antimicrobial Effectiveness Testing (AET) was performed at 0 and 180 days
after compounding.

Results:
According to our results, BUDs of up to 90-180 days were obtained for the examined formulations
stored at room temperature, considering a degradation of maximum 10% of the nominal
concentration of the APIs within them. The formulations exhibited no discernible physical alterations
throughout this period and maintained chemical stability within acceptable limits. Microbiological
evaluations confirmed the efficacy of the preservative system.

Conclusion:
Products compounded with TrichoSol™ and TrichoFoam™ showed suitable stability to
be used as personalized treatments for alopecia. We can then suggest that the vehicles TrichoSol™
and TrichoFoam™ present effective solutions for compounding personalized hair care treatments.