bluuebunny/crossref_metadata_2025_split huggingface dataset:
Online information acquisition affects food risk prevention behaviours: the roles of topic concern, information credibility and risk perception
Authors: You Zhenwu , Zhan Weizhen , Zhang Fan 
Published: October 2023

Abstract
Background
The COVID-19 pandemic has not only brought great challenges to the global health system but also bred numerous rumours about food safety. Food safety issues have once again attracted public attention.

Methods
The data were drawn from the fifth wave of the first Taiwan Communication Survey database. The respondents were selected via multistage stratified random sampling. The sampling units were townships/districts, villages/neighbourhoods and households. The sample consisted of 2098 respondents. This study first used propensity value matching to analyse the direct impact of online food safety information acquisition on preventive behaviours and examined the heterogeneous impact caused by the difference in the degree of topic attention through value matching. Hayes’ PROCESS macro model 6 was applied to confirm the mediating effect and the serial mediating effect.

Results
The research results show that an increase in the frequency of the acquisition of online food safety information significantly increases individuals’ food risk prevention behaviour. However, only users with high concern about the issue are affected. The food risk prevention behaviour of users with low concern about this issue is not affected by the acquisition of online food safety information. Further analysis shows that risk perception and information credibility both play mediating roles in the impact of online food safety information acquisition on food risk prevention behaviour. Moreover, the transmission and united effects of information credibility and risk perception play a distal mediating role.

Conclusions
Food risk prevention behaviours are an important topic for personal health as well as government management. Our study’s findings can provide empirical evidence for risk managers and decision-makers to reevaluate the role of the internet in food risk management.
Validating a measure for eco-anxiety in Portuguese young adults and exploring its associations with environmental action
Authors: Sampaio Francisco , Costa Tiago , Teixeira-Santos Luísa , de Pinho Lara Guedes , Sequeira Carlos , Luís Sílvia , Loureiro Ana , Soro Jerônimo C. , Roldán Merino Juan , Moreno Poyato Antonio , Peña Loray Juan Segundo , Rodríguez Quiroga Andrea , O’Brien Léan V. , Hogg Teaghan L. , Stanley Samantha K. 
Published: October 2023

Abstract
Background
Worsening environmental conditions may amplify people’s emotional responses to an environmental crisis (eco-anxiety). In Portugal, young people seem to be especially concerned about climate change. However, this phenomenon needs to be interpreted using accurate instruments. Thus, this study aimed to validate the Portuguese version of the Hogg Eco-Anxiety Scale (HEAS) in young adults and examine the associations among eco-anxiety, sociodemographic characteristics, and pro-environmental behaviours.

Methods
A survey was administered to 623 Portuguese university students aged between 18 and 25 years. The survey included our Portuguese translation of the HEAS (obtained through a back-translation and pretesting process), a sociodemographic assessment, and questions related to pro-environmental behaviours. Confirmatory factor analysis was conducted to assess the construct validity of the Portuguese version of the HEAS, and global fit indices were used to assess whether the original four-dimensional structure of the scale was reproduced. The reliability of the Portuguese version of the HEAS was evaluated by Cronbach’s alpha and the intraclass correlation coefficient. Measurement invariance examined sex differences in scale interpretation. Linear regressions were used to detect whether sociodemographic variables predict eco-anxiety and whether eco-anxiety predicts pro-environmental behaviours.

Results
The factorial structure of the original scale was replicated in the Portuguese version of the HEAS, showing good internal consistency, reliability over time and strict invariance between men and women. A higher paternal education level predicted greater eco-anxiety in children. Two dimensions of eco-anxiety—namely, rumination and anxiety about personal impacts on the environment—predicted higher engagement in pro-environmental behaviours.

Conclusions
The translated scale is an appropriate tool to measure eco-anxiety in the Portuguese context and should be used to collect evidence to drive environmental and health policies. An individual’s education level should be considered a determinant of their emotional response to environmental conditions. Importantly, eco-anxiety can act as a protective emotional response to preserving the planet.
Study on proximal humerus evaluation of effective treatment (SPHEER) – what is the effect of rehabilitation compliance on clinical outcomes of proximal humerus fractures
Authors: Nah Ming Foo Kenneth , Pereira Michelle Jessica , Hemaavathi Mani , Wong Shiyun Winnie , Lim Chien Joo , Tan Bryan Yijia 
Published: October 2023

Abstract
Introduction
Proximal humeral fractures (PHFs) are 3rd commonest fragility fractures and cause significant functional impairment. This paper sought to determine impact of rehabilitation compliance on the clinical outcomes for non-surgically managed PHFs, while ascertaining reasons for non-compliance which can be addressed to improve compliance.

Methods
Prospective cohort study of patients undergoing non-surgical treatment for PHFs from August 2017 to April 2020 in a tertiary trauma centre was performed. Data was collected via questionnaire: patient demographic data, PHF injury details, clinical outcome measures, therapist-reported (Sport Injury Rehabilitation Adherence Scale [SIRAS]) and patient-reported (subjective compliance, frequency of exercise) rehabilitation compliance measures. Data was analysed using multiple linear regression model to account for confounding variables.

Results
107 participants attended physical therapy follow-up for mean 137.8 days. 6-week SIRAS strongly predicted 3-month Constant score (p = 0.023; 95%CI = 0.265,3.423), OSS (p = 0.038; 95%CI = 0.049,1.634), flexion ROM (p < 0.001; 95%CI = 2.872,8.982), extension ROM (p = 0.035; 95%CI = 0.097,2.614), abduction ROM (p = 0.002;95%CI = 1.995,8.466) and achievement of functional active ROM at 3-months (p = 0.049; 95%CI = 1.001,1.638). Pain was the top reason impairing rehabilitation compliance from therapist (43.9% at 6-weeks and 20.6% at 3-months) and patient-perspective (33.6% at 6-weeks, 24.3% at 3-months). Author-developed patient-reported compliance measures had good correlation with validated SIRAS score (subjective compliance: p < 0.001 frequency of exercise: p = 0.001).

Conclusion
Rehabilitation compliance predicts short-term clinical outcomes up to 3-months and potentially 1-year outcomes. Pain control should be optimised to maximise rehabilitation compliance and improve PHF outcomes. There is lack of consensus definition for rehabilitation compliance measures; patient-reported measures used have good correlation to existing validated measures and could serve as a steppingstone for further research.

Level of evidence
II, cohort study.
Outcomes of direct anterior approach for uncemented total hip replacement in medial femoral neck fractures: a retrospective comparative study on the first 100 consecutive patients
Authors: Di Martino Alberto , Pederiva Davide , Brunello Matteo , Tassinari Leonardo , Geraci Giuseppe , Stefanini Niccolò , Faldini Cesare 
Published: October 2023

Abstract
Background
With the aging of the population, the incidence of medial femoral neck fractures is likely to increase, and along them the need for total hip replacement. The present study aimed to analyze whether the use of the direct anterior hip approach, compared with posterolateral approach in medial proximal femur fracture patients, results in any advantage in terms of complications rate and functional recovery.

Methods
A total of 162 patients were included in the study, and divided by approach: 100 performed with direct anterior approach (group A) and 62 with posterolateral approach (group B). The two populations were overlapping in age (75 vs 74 years; p = 0.13), sex (58F 42M vs 46F 16M; p = 0.12) and BMI (24 vs 24; p = 0.77).

Results
Group A showed a higher ASA score compared to group B (3 vs 2; p = 0.04). Similar hospital stays (7 vs 7 days; p = 0.55) and complication rates (6% vs 8%; p = 0.61) were observed among groups, the most frequent being periprosthetic fractures, and need for allogeneic blood transfusion (20% vs 13%; p = 0.25). Patients in group A (96 vs 85 min; p = 0.10) showed a slightly, longer surgical time and a faster postoperative functional recovery witnessed by the ability to climb stairs at hospital discharge (37% vs 21%; p = 0.041).

Conclusion
The use of the direct anterior hip approach was effective in the management of frail patients with medial femoral neck fractures managed by total hip arthroplasty, allowing faster functional recovery in the elderly population.
Low proviral load in the Kumamoto strain of Japanese Brown cattle infected with the bovine leukemia virus
Authors: Inenaga Toshiaki , Fukuoka Koh , Sumida Mikiya , Aiba Sakiko , Nishikaku Kohei , Matsuno Yuta , Kobayashi Tomoko , Imakawa Kazuhiko 
Published: October 2023

Abstract
Background
The Kumamoto strain of Japanese Brown (JBRK) cattle is a sub-breed of Wagyu and has a different genetic background than that of Japanese Black (JB) cattle. Bovine leukemia virus (BLV) is the pathogen causing enzootic bovine leukosis (EBL), the predominant type of bovine leukosis (BL). EBL is one of the most common bovine infectious diseases in dairy countries, including Japan. Some host genetic factors, including the bovine leukocyte antigen (BoLA)-DRB3 gene, have been associated with the proviral load (PVL) of BLV and/or onset of EBL. Here, we determined the number of BL cases by analyzing prefectural case records in detail. We measured the PVL of BLV-infected JBRK cattle and compared it with that obtained for other major breeds, JB and Holstein-Friesian (HF) cattle. Finally, the relationship between PVL levels and BoLA-DRB3 haplotypes was investigated in BLV-infected JBRK cattle.

Results
We determined the number of BL cases recorded over the past ten years in Kumamoto Prefecture by cattle breed. A limited number of BL cases was observed in JBRK cattle. The proportion of BL cases in the JBRK was lower than that in JB and HF. The PVL was significantly lower in BLV-infected JBRK cattle than that in the JB and HF breeds. Finally, in BLV-infected JBRK cattle, the PVL was not significantly affected by BoLA-DRB3 alleles and haplotypes. BoLA-DRB3 allelic frequency did not differ between BLV-infected JBRK cattle with low PVL and high PVL.

Conclusions
To our knowledge, this is the first report showing that BL occurred less in the JBRK population of Kumamoto Prefecture. After BLV-infection, the PVL was significantly lower in JBRK cattle than that in JB and HF breeds. The genetic factors implicated in maintaining a low PVL have yet to be elucidated, but the BoLA-DRB3 haplotypes are likely not involved.
A macrophage related signature for predicting prognosis and drug sensitivity in ovarian cancer based on integrative machine learning
Authors: Zhao Bo , Pei Lipeng 
Published: October 2023

Abstract
Background
Ovarian cancer ranks the leading cause of gynecologic cancer-related death in the United States and the fifth most common cause of cancer-related mortality among American women. Increasing evidences have highlighted the vital role of macrophages M2/M1 proportion in tumor progression, prognosis and immunotherapy.

Methods
Weighted gene co-expression network analysis (WGCNA) was performed to identify macrophages related markers. Integrative procedure including 10 machine learning algorithms were performed to develop a prognostic macrophage related signature (MRS) with TCGA, GSE14764, GSE140082 datasets. The role of MRS in tumor microenvironment (TME) and therapy response was evaluated with the data of CIBERSORT, MCPcounter, QUANTISEQ, XCELL, CIBERSORT-ABS, TIMER and EPIC, GSE91061 and IMvigor210 dataset.

Results
The optimal MRS developed by the combination of CoxBoost and StepCox[forward] algorithm served as an independent risk factor in ovarian cancer. Compared with stage, grade and other established prognostic signatures, the current MRS had a better performance in predicting the overall survival rate of ovarian cancer patients. Low risk score indicated a higher TME score, higher level of immune cells, higher immunophenoscore, higher tumor mutational burden, lower TIDE score and lower IC50 value in ovarian cancer. The survival prediction nomogram had a good potential for clinical application in predicting the 1-, 3-, and 5-year overall survival rate of ovarian cancer patients.

Conclusion
All in all, the current study constructed a powerful prognostic MRS for ovarian cancer patients using 10 machine learning algorithms. This MRS could predict the prognosis and drug sensitivity in ovarian cancer.
Native American ancestry and breast cancer risk in Colombian and Mexican women: ruling out potential confounding through ancestry-informative markers
Authors: Zollner Linda , Torres Diana , Briceno Ignacio , Gilbert Michael , Torres-Mejía Gabriela , Dennis Joe , Bolla Manjeet K. , Wang Qin , Hamann Ute , Lorenzo Bermejo Justo 
Published: October 2023

Abstract
Background
Latin American and Hispanic women are less likely to develop breast cancer (BC) than women of European descent. Observational studies have found an inverse relationship between the individual proportion of Native American ancestry and BC risk. Here, we use ancestry-informative markers to rule out potential confounding of this relationship, estimating the confounder-free effect of Native American ancestry on BC risk.

Methods and study population
We used the informativeness for assignment measure to select robust instrumental variables for the individual proportion of Native American ancestry. We then conducted separate Mendelian randomization (MR) analyses based on 1401 Colombian women, most of them from the central Andean regions of Cundinamarca and Huila, and 1366 Mexican women from Mexico City, Monterrey and Veracruz, supplemented by sensitivity and stratified analyses.

Results
The proportion of Colombian Native American ancestry showed a putatively causal protective effect on BC risk (inverse variance-weighted odds ratio [OR] = 0.974 per 1% increase in ancestry proportion, 95% confidence interval [CI] 0.970–0.978, p = 3.1 × 10–40). The corresponding OR for Mexican Native American ancestry was 0.988 (95% CI 0.987–0.990, p = 1.4 × 10–44). Stratified analyses revealed a stronger association between Native American ancestry and familial BC (Colombian women: OR = 0.958, 95% CI 0.952–0.964; Mexican women: OR = 0.973, 95% CI 0.969–0.978), and stronger protective effects on oestrogen receptor (ER)-positive BC than on ER-negative and triple-negative BC.

Conclusions
The present results point to an unconfounded protective effect of Native American ancestry on BC risk in both Colombian and Mexican women which appears to be stronger for familial and ER-positive BC. These findings provide a rationale for personalised prevention programmes that take genetic ancestry into account, as well as for future admixture mapping studies.
The multi-kingdom microbiome of the goat gastrointestinal tract
Authors: Cao Yanhong , Feng Tong , Wu Yingjian , Xu Yixue , Du Li , Wang Teng , Luo Yuhong , Wang Yan , Li Zhipeng , Xuan Zeyi , Chen Shaomei , Yao Na , Gao Na L. , Xiao Qian , Huang Kongwei , Wang Xiaobo , Cui Kuiqing , Rehman Saif ur , Tang Xiangfang , Liu Dewu , Han Hongbing , Li Ying , Chen Wei-Hua , Liu Qingyou 
Published: October 2023

Abstract
Background
Goat is an important livestock worldwide, which plays an indispensable role in human life by providing meat, milk, fiber, and pelts. Despite recent significant advances in microbiome studies, a comprehensive survey on the goat microbiomes covering gastrointestinal tract (GIT) sites, developmental stages, feeding styles, and geographical factors is still unavailable. Here, we surveyed its multi-kingdom microbial communities using 497 samples from ten sites along the goat GIT.

Results
We reconstructed a goat multi-kingdom microbiome catalog (GMMC) including 4004 bacterial, 71 archaeal, and 7204 viral genomes and annotated over 4,817,256 non-redundant protein-coding genes. We revealed patterns of feeding-driven microbial community dynamics along the goat GIT sites which were likely associated with gastrointestinal food digestion and absorption capabilities and disease risks, and identified an abundance of large intestine-enriched genera involved in plant fiber digestion. We quantified the effects of various factors affecting the distribution and abundance of methane-producing microbes including the GIT site, age, feeding style, and geography, and identified 68 virulent viruses targeting the methane producers via a comprehensive virus-bacterium/archaea interaction network.

Conclusions
Together, our GMMC catalog provides functional insights of the goat GIT microbiota through microbiome-host interactions and paves the way to microbial interventions for better goat and eco-environmental qualities.
Carbon quantum dots as a tracer of water seepage sources and pathways in grottoes
Authors: Sun Bo , Ma Fenyan , Zhang Huyuan , Peng Ningbo , Zhang Peng 
Published: October 2023

Abstract
Water seepage is one of the main factors leading to the damage of grottoes. The sources and pathways of water seepage need to be identified to relieve it. Although the sources and pathways are investigated using geophysical exploration methods commonly, the results are unsatisfactory due to the limitation of resolution. The tracer method has been widely used to examine water seepage in the natural sciences and engineering. However, most tracers have an impact on grottoes, making this method inapplicable. This study was the first to use the carbon quantum dots as a tracer of water seepage in grottoes. The characteristics of the carbon quantum dots, which was synthesized by various biomass precursors through large-scale synthesis in the field, were analyzed to determine the optimal precursor. The structure, fluorescence intensity, and water solubility of the carbon quantum dots were evaluated. Laboratory tests were designed to examine the transport properties of the carbon quantum dots in rocks and cracks. The results showed that the carbon quantum dots synthesized by Ginkgo biloba were small and had uniform size, excellent fluorescence, good water solubility and transport ability. Furthermore, the carbon quantum dots were successfully used to tracing the source of water seepage at the chest of the Leshan Giant Buddha. The low cost of synthesis, wide precursors, easy and convenient synthesis methods, friendliness to grottoes, and excellent performance of the carbon quantum dots as a tracer suggest the efficacy of this method. These findings could lead to the widespread use of tracer method in studies of water seepage in grottoes.
Activation of multiple Eph receptors on neuronal membranes correlates with the onset of optic neuropathy
Authors: Strong Thomas A. , Esquivel Juan , Wang Qikai , Ledon Paul J. , Wang Hua , Gaidosh Gabriel , Tse David , Pelaez Daniel 
Published: October 2023

Abstract
Background
Optic neuropathy is a major cause of irreversible blindness, yet the molecular determinants that contribute to neuronal demise have not been fully elucidated. Several studies have identified ‘ephrin signaling’ as one of the most dysregulated pathways in the early pathophysiology of optic neuropathy with varied etiologies. Developmentally, gradients in ephrin signaling coordinate retinotopic mapping via repulsive modulation of cytoskeletal dynamics in neuronal membranes. Little is known about the role ephrin signaling plays in the post-natal visual system and its correlation with the onset of optic neuropathy.

Methods
Postnatal mouse retinas were collected for mass spectrometry analysis for erythropoietin-producing human hepatocellular (Eph) receptors. Optic nerve crush (ONC) model was employed to induce optic neuropathy, and proteomic changes during the acute phase of neuropathic onset were analyzed. Confocal and super-resolution microscopy determined the cellular localization of activated Eph receptors after ONC injury. Eph receptor inhibitors assessed the neuroprotective effect of ephrin signaling modulation.

Results
Mass spectrometry revealed expression of seven Eph receptors (EphA2, A4, A5, B1, B2, B3, and B6) in postnatal mouse retinal tissue. Immunoblotting analysis indicated a significant increase in phosphorylation of these Eph receptors 48 h after ONC. Confocal microscopy demonstrated the presence of both subclasses of Eph receptors within the retina. Stochastic optical reconstruction microscopy (STORM) super-resolution imaging combined with optimal transport colocalization analysis revealed a significant co-localization of activated Eph receptors with injured neuronal cells, compared to uninjured neuronal and/or injured glial cells, 48 h post-ONC. Eph receptor inhibitors displayed notable neuroprotective effects for retinal ganglion cells (RGCs) after six days of ONC injury.

Conclusions
Our findings demonstrate the functional presence of diverse Eph receptors in the postnatal mammalian retina, capable of modulating multiple biological processes. Pan-Eph receptor activation contributes to the onset of neuropathy in optic neuropathies, with preferential activation of Eph receptors on neuronal processes in the inner retina following optic nerve injury. Notably, Eph receptor activation precedes neuronal loss. We observed a neuroprotective effect on RGCs upon inhibiting Eph receptors. Our study highlights the importance of investigating this repulsive pathway in early optic neuropathies and provides a comprehensive characterization of the receptors present in the developed retina of mice, relevant to both homeostasis and disease processes.
Risk of mental disorders and malnutrition in elderly COVID-19 survivors: An observational study
Authors: Theresa Ria Maria , Dewiastuti Marlina , Ningsih Sri Rahayu , Safira Lisa 
Published: October 2023

Background: The incidence rate of COVID-19 is around 11-15% in the elderly. The case fatality rate (CFR) of COVID-19 in the elderly is around 8.9% and increases with age. The risk of mental disorders and malnutrition is increased in COVID-19 survivors. Continuous inflammatory conditions result in a state of hypercatabolism that can disrupt brain neuroendocrine and protein consumption for the formation of acute-phase reactant proteins. Mental disorders and malnutrition can lead to fragility. The aim of this study was to assess the risk of mental disorders and malnutrition in elderly survivors of COVID-19. Methods: This research was a cross-sectional study. The results of the research on age, disease symptoms, and comorbidities have proven that they are risk factors for mental disorders and malnutrition in elderly COVID-19 survivors. This study used total sampling and included 100 study subjects. The research was conducted in Depok for two months; data was collected directly through shared questionnaires and direct anthropometric measurements. The questionnaires used were the SRQ-20 tool for mental disorder screening and MNA for malnutrition screening. Results: The risk factors for mental disorders were age over 70 years old OR 3 (CI 1.0-8.8), severe COVID-19 symptoms OR 4.5 (CI 1.2-16.17), and multi-comorbidity OR 2.3 (CI 0.6-8.8). The risk factors for malnutrition were age higher than 70 years old OR 2.5 (CI 0.8-7.9), moderate COVID-19 symptoms OR 6.3 (CI 2.0-19.81), and multi-comorbidity OR 6.6 (CI 1.5-28.5). Conclusions: Those infected with COVID-19 have a risk of mental disorders and malnutrition, especially in geriatrics, and this risk increases with age.