bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mangiferin (mango) attenuates AOM-induced colorectal cancer in rat’s colon by augmentation of apoptotic proteins and antioxidant mechanisms
Authors: Abdul-Aziz Ahmed Khaled , Jabbar Ahmed A. J. , Abdulla Mahmood Ameen , Zuhair Alamri Zaenah , Ain Salehen Nur , Abdel Aziz Ibrahim Ibrahim , Almaimani Ghassan , Bamagous Ghazi A. , Almaimani Riyad A. , Almasmoum Hussain A. , Ghaith Mazen M. , Farrash Wesam F. 
Published: January 2024

AbstractMangiferin (MF) is a natural C-glucosylxantone compound that has many substantial curative potentials against numerous illnesses including cancers. The present study's goal is to appraise the chemo preventive possessions of MF on azoxymethane (AOM)-mediated colonic aberrant crypt foci (ACF) in rats. Rats clustered into 5 groups, negative control (A), inoculated subcutaneously with normal saline twice and nourished on 0.5% CMC; groups B-E injected twice with 15 mg/kg azoxymethane followed by ingestion of 0.5% CMC (B, cancer control); intraperitoneal inoculation of 35 mg/kg 5-fluorouracil (C, reference rats) or nourished on 30 mg/kg (D) and 60 mg/kg (E) of MF. Results of gross morphology of colorectal specimens showed significantly lower total colonic ACF incidence in MF-treated rats than that of cancer controls. The colon tissue examination of cancer control rats showed increased ACF availability with bizarrely elongated nuclei, stratified cells, and higher depletion of the submucosal glands compared to MF-treated rats. Mangiferin treatment caused increased regulation of pro-apoptotic (increased Bax) proteins and reduced the β-catenin) proteins expression. Moreover, rats fed on MF had significantly higher glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and lower malondialdehyde (MDA) concentrations in their colonic tissue homogenates. Mangiferin supplementation significantly down-shifted pro-inflammatory cytokines (transforming growth factor-α and interleukine-6) and up-shifted anti-inflammatory cytokines (interleukine-10) based on serum analysis. The chemo-protective mechanistic of MF against AOM-induced ACF, shown by lower ACF values and colon tissue penetration, could be correlated with its positive modulation of apoptotic cascade, antioxidant enzymes, and inflammatory cytokines originating from AOM oxidative stress insults.
Genetically engineered HEK cells as a valuable tool for studying electroporation in excitable cells
Authors: Batista Napotnik Tina , Kos Bor , Jarm Tomaž , Miklavčič Damijan , O’Connor Rodney P. , Rems Lea 
Published: January 2024

AbstractElectric pulses used in electroporation-based treatments have been shown to affect the excitability of muscle and neuronal cells. However, understanding the interplay between electroporation and electrophysiological response of excitable cells is complex, since both ion channel gating and electroporation depend on dynamic changes in the transmembrane voltage (TMV). In this study, a genetically engineered human embryonic kidney cells expressing NaV1.5 and Kir2.1, a minimal complementary channels required for excitability (named S-HEK), was characterized as a simple cell model used for studying the effects of electroporation in excitable cells. S-HEK cells and their non-excitable counterparts (NS-HEK) were exposed to 100 µs pulses of increasing electric field strength. Changes in TMV, plasma membrane permeability, and intracellular Ca2+ were monitored with fluorescence microscopy. We found that a very mild electroporation, undetectable with the classical propidium assay but associated with a transient increase in intracellular Ca2+, can already have a profound effect on excitability close to the electrostimulation threshold, as corroborated by multiscale computational modelling. These results are of great relevance for understanding the effects of pulse delivery on cell excitability observed in context of the rapidly developing cardiac pulsed field ablation as well as other electroporation-based treatments in excitable tissues.
Ca2+ and force during dynamic contractions in mouse intact skeletal muscle fibers
Authors: Fukutani Atsuki , Westerblad Håkan , Jardemark Kent , Bruton Joseph 
Published: January 2024

AbstractMuscle fiber force production is determined by the excitation frequency of motor nerves, which induce transient increases in cytoplasmic free Ca2+ concentration ([Ca2+]i) and the force-generating capacity of the actomyosin cross-bridges. Previous studies suggest that, in addition to altered cross-bridge properties, force changes during dynamic (concentric or eccentric) contraction might be affected by Ca2+-dependent components. Here we investigated this by measuring [Ca2+]i and force in mouse muscle fibers undergoing isometric, concentric, and eccentric contractions. Intact single muscle fibers were dissected from the flexor digitorum brevis muscle of mice. Fibers were electrically activated isometrically at 30–100 Hz and after reaching the isometric force plateau, they were actively shortened or stretched. We calculated the ratio (relative changes) in force and [Ca2+]i attained in submaximal (30 Hz) and near-maximal (100 Hz) contractions under isometric or dynamic conditions. Tetanic [Ca2+]i was similar during isometric, concentric and eccentric phases of contraction at given stimulation frequencies while the forces were clearly different depending on the contraction types. The 30/100 Hz force ratio was significantly lower in the concentric (44.1 ± 20.3%) than in the isometric (50.3 ± 20.4%) condition (p = 0.005), whereas this ratio did not differ between eccentric and isometric conditions (p = 0.186). We conclude that the larger force decrease by decreasing the stimulation frequency during concentric than during isometric contraction is caused by decreased myofibrillar Ca2+ sensitivity, not by the decreased [Ca2+]i.
Analysis of the maternal inheritance hypothesis of the exochorium in eggs from hybrids of Chagas disease vectors
Authors: de Sousa Paulo Sergio , de Oliveira Jader , Ravazi Amanda , dos Reis Yago Visinho , de Azeredo Oliveira Maria Tercília Vilela , da Rosa João Aristeu , Galvão Cleber , Alevi Kaio Cesar Chaboli 
Published: January 2024

AbstractMorphological studies applied to the taxonomy of the Triatominae cover various structures (head, wing, thorax, genitalia, and eggs). Exochorial structures of hybrid eggs were characterized and compared with the parents, demonstrating that hybrids presented characteristics identical to the exochorial pattern observed in the females of the crosses, which resulted in the hypothesis that the pattern of triatomine eggs is possibly a characteristic inherited from females. Thus, we characterized the exochorium of the eggs of several triatomine hybrids and compared them with the parents, to assess the pattern of segregation and test the hypothesis of maternal inheritance. Hybrids were obtained in at least one direction from all crosses. The analysis of the exochorium of the eggs of the hybrids showed different patterns of segregation: "exclusively paternal", "predominantly maternal", "predominantly paternal", "mutual", and "differential". Curiously, none of the hybrids evaluated presented characteristics that segregated exclusively from the female parental species. Thus, we demonstrate that the hypothesis of maternal inheritance of the exochorium pattern of eggs is not valid and we emphasize the importance of alternative/combined tools (such as integrative taxonomy) for the correct identification of these insect vectors (mainly in view of possible natural hybridization events due to climate and environmental changes).
QR-1011 restores defective ABCA4 splicing caused by multiple severe ABCA4 variants underlying Stargardt disease
Authors: Kaltak Melita , de Bruijn Petra , van Leeuwen Willemijn , Platenburg Gerard , Cremers Frans P. M. , Collin Rob W. J. , Swildens Jim 
Published: January 2024

AbstractStargardt disease type 1 (STGD1), the most common form of hereditary macular dystrophy, can be caused by biallelic combinations of over 2200 variants in the ABCA4 gene. This leads to reduced or absent ABCA4 protein activity, resulting in toxic metabolite accumulation in the retina and damage of the retinal pigment epithelium and photoreceptors. Approximately 21% of all ABCA4 variants that contribute to disease influence ABCA4 pre-mRNA splicing. This emphasizes the need for therapies to restore disrupted ABCA4 splicing and halt STGD1 progression. Previously, QR-1011, an antisense oligonucleotide (AON), successfully corrected splicing abnormalities and restored normal ABCA4 protein translation in human retinal organoids carrying the prevalent disease-causing variant c.5461−10T>C in ABCA4. Here, we investigated whether QR-1011 could also correct splicing in four less common non-canonical splice site (NCSS) variants flanking ABCA4 exon 39: c.5461−8T>G, c.5461−6T>C, c.5584+5G>A and c.5584+6T>C. We administered QR-1011 and three other AONs to midigene-transfected cells and demonstrate that QR-1011 had the most pronounced effect on splicing compared to the others. Moreover, QR-1011 significantly increased full-length ABCA4 transcript levels for c.5461−8T>G and c.5584+6T>C. Splicing restoration could not be achieved in the other two variants, suggesting their more severe effect on splicing. Overall, QR-1011, initially developed for a single ABCA4 variant, exhibited potent splice correction capabilities for two additional severe NCSS variants nearby. This suggests the possibility of a broader therapeutic impact of QR-1011 extending beyond its original target and highlights the potential for treating a larger population of STGD1 patients affected by multiple severe ABCA4 variants with a single AON.
Big data-driven spatio-temporal heterogeneity analysis of Beijing's catering service industry during the COVID-19 pandemic
Authors: Jia Haichao , Zheng Minrui , Wang Peipei , Li Tianle , Zheng Xinqi 
Published: January 2024

AbstractThe Catering Service Industry (CSI) experienced profound impacts due to the COVID-19 pandemic. However, the long-term and multi-timepoint analysis using big data remained limited, influencing governmental decision-making. We applied Kernel Density Estimation, Shannon Diversity Index, and the Geographic detector to explore the spatial heterogeneity and determinants of the CSI in Beijing during the pandemic, with monthly granularity. The temporal-spatial dynamics of the CSI presented a "W"-shaped trend from 2018 to 2023, with pivotal shifts aligning with key pandemic stages. Spatial characteristics exhibited heterogeneity, with greater stability in the city center and more pronounced shifts in peripheral urban zones. Districts facing intricate outbreaks showed lower catering income, and Chinese eateries exhibited heightened resilience compared to others. The CSI displayed strong interconnections with living service sectors. Development in each district was influenced by economic level, population distribution, service facilities convenience, and the risk of the COVID-19 pandemic. Dominant factors included total retail sales of consumer goods, permanent population, average Baidu Heat Index, density of transportation and catering service facilities, infection cases and the consecutive days with confirmed cases existing. Consequently, we suggested seizing post-pandemic recovery as an avenue to unlock the CSI's substantial potential, ushering a fresh phase of growth.
Analysis and experimental research of transient temperature rise characteristics of high-speed cylindrical roller bearing
Authors: Dong Yanfang , Ma Yafei , Qiu Ming , Chen Feifan , He Kai 
Published: January 2024

AbstractIn this paper, on one hand, the time-varying characteristics of the heat source and thermal boundary conditions of the high-speed spindle system were analyzed considering the thermal-structural coupling effect. And a transient bearing temperature field prediction method combining the thermal network method and finite element method was proposed. Furthermore, the relationships between time step, calculation efficiency and calculation results were analyzed. On the other hand, a online real-time monitoring system of the transient temperature of the cylindrical roller bearing inner ring for maximum speed of 13,000 r/min was designed and implemented using fibre optic sensing technology. Comparing with the conventional static thermal analysis results, it is verified that the simulation method proposed in this paper has higher accuracy. This paper provides a new approach for analysing and testing the thermal characteristics of high-speed spindle system.
Domestic pigs are susceptible to experimental infection with non-human primate-derived Reston virus without the need for adaptation
Authors: Lewis Charles E. , Pinette Mathieu M. , Lakin Steven M. , Smith Greg , Fisher Mathew , Moffat Estella , Embury-Hyatt Carissa , Pickering Brad S. 
Published: January 2024

AbstractDomestic pigs are a critical component of the food supply and one of the most commonly raised production animals. Pork consumption has driven the intensification of pig production expanding into environments conducive to increased emergence and spread of infectious diseases, including the spillover of pathogens into human populations. One of these emerging viruses, Reston virus (RESTV), is an enigma among the Orthoebolavirus genus in that its lack of human pathogenicity is in stark contrast to the high virulence associated with most other ebolaviruses. RESTV is, however, associated with outbreaks of highly lethal hemorrhagic disease in non-human primates (NHP), as well as poorly understood clinical manifestations of mixed virulence and lethality in naturally and experimentally infected domestic pigs. Our results show it is possible for RESTV derived from an NHP to infect domestic pigs resulting in a spectrum of disease, from asymptomatic to severe respiratory distress. Further, we report on the first experimental transmission of RESTV between infected pigs and a co-housed, naïve animal, as well as the first report of the successful use of group oral fluids for the detection of RESTV RNA and virus-specific IgA antibodies.
The role of implants and implant prostheses on the accuracy and artifacts of cone-beam computed tomography: an in-vitro study
Authors: Gurjar Balwant Singh , Sharma Vineet , Paliwal Jyoti , Kalla Rajani , Meena Kamal Kumar , Tahir Mohammed 
Published: January 2024

AbstractTo assess the accuracy of CBCT in implant-supported prostheses and to evaluate metal artifacts with and without implants or implant prostheses. Accuracy and artifacts were assessed in the dried mandible at three points on the buccal and lingual cortical plates on the mandible's body near the crest and the base. On the buccal cortical plate, these points were labelled as A, B and C near the crest and D, E and F near the base of the body of the mandible. Similarly, points a to f were marked on the lingual cortical plate corresponding to points A to F. The study had two control groups, C0 for physical linear measurement (PLM) and C1 for radiographic linear measurement (RLM) and artifact assessment. There were seven test groups, TG 1 to 7, progressing from a single implant to implant full-arch prosthesis. For accuracy assessment, PLM was compared to RLM. CBCT artifacts were investigated in images integrated at 0.25 mm, 10 mm, and 20 mm at regions of interest on concentric circles at different intersecting angles by comparing grayscale values at C1 and TG1 to 7. The data were collected and statistically analyzed. A significant difference was observed between C0 and C1, and RLM in test groups at the superior axial plane. Similarly, PLM and test RLM in the sagittal plane at A-B, B-C, and D-E were statistically significant. A significant difference between PLM and RLM was also observed in the vertical plane at A-D, B-E, and C-F. Quantification of CBCT artifacts in the presence of implants or prostheses revealed that full-arch prostheses had the highest mean grayscale value, whereas single implants with a prosthesis had the lowest. The mean grayscale change was greatest around the implant and implant prosthesis. The mean grayscale value was maximum at 20 mm voxel integration scales (VIS) and lowest at 0.25 mm. CBCT is a clinically reliable device. Metal in implants or implant-supported prostheses prevents true assessment of the peri-implant area; therefore, lower VIS is suggested in the presence of implants or implant prostheses.
New energy vehicle battery recycling strategy considering carbon emotion from a closed-loop supply chain perspective
Authors: Guo Rong , He Yongjun , Tian Xianjun , Li Yixin 
Published: January 2024

AbstractThe negative impact of used batteries of new energy vehicles on the environment has attracted global attention, and how to effectively deal with used batteries of new energy vehicles has become a hot issue. This paper combines the rank-dependent expected utility with the evolutionary game theory, constructs an evolutionary game model based on the interaction mechanism between decision makers' emotions and decision making, and studies the recycling strategy of new energy automobile trams under the heterogeneous combination of emotions. The study shows that: (1) In addition to the establishment of effective external norms, the subjective preference of decision makers can also positively affect the recycling strategy of new energy vehicle batteries. (2) Fairness preferences can have a significant nonlinear effect on new energy vehicle battery recycling strategies by changing the utility function of decision makers. (3) When new energy vehicle manufacturers remain optimistic and new energy vehicle demanders remain rational or pessimistic, the new energy vehicle battery recycling strategy can reach the optimal steady state.
Enhancing engineering optimization using hybrid sine cosine algorithm with Roulette wheel selection and opposition-based learning
Authors: Pham Vu Hong Son , Nguyen Dang Nghiep Trinh , Nguyen Van Nam 
Published: January 2024

AbstractMeta-heuristic algorithms distinguish themselves from conventional optimization methods owing to their intrinsic adaptability and straightforward implementation. Among them, the sine cosine algorithm (SCA) is lauded for its ability to transition seamlessly between exploration and exploitation phases throughout the optimization process. However, there exists potential for enhancing the balance that SCA maintains between exploration and exploitation. To augment the proficiency in global optimization of SCA, an innovative strategy—nSCA—that integrates the roulette wheel selection (RWS) with opposition-based learning was formulated. The robustness of nSCA was rigorously evaluated against leading-edge methods such as the genetic algorithm (GA), particle swarm optimization, moth-flame optimization, ant lion optimization, and multi-verse optimizer, as well as the foundational SCA. This evaluation included benchmarks set by both CEC 2019 and CEC 2021 test functions. Additionally, the performance of nSCA was confirmed through numerous practical optimization problems, emphasizing its effectiveness in applied settings. In all evaluations, nSCA consistently showcased superior performance compared to its evolutionary algorithm counterparts, delivering top-tier solutions for both benchmark functions and real-world optimization challenges. Given this compelling evidence, one can posit that nSCA serves as a strong candidate for addressing intricate optimization challenges found in real-world contexts, regardless of whether they are of a discrete or continuous nature.
A method for extracting small water bodies based on DEM and remote sensing images
Authors: Sun Qingzhen , Li Jinguang 
Published: January 2024

AbstractAccurate and comprehensive extraction of water bodies facilitates the study of watershed changes and water quality monitoring and also provides a reference for ecological evolution. When extracting tributaries and small water bodies based on remote sensing images, there are problems such as uncertain threshold selection, more edge voids, and extraction difficulties. The improved water body extraction method (Combination Normalized Difference Water Index), fuses Digital Elevation Model and remote sensing images. The river network is extracted from the Digital Elevation Model and the remote sensing image is normalized. After fusion, threshold comparison is performed, and the threshold of the river network location is used as the basis for connecting the broken water bodies, it is compared and verified with the normalized thresholds to achieve accurate identification and complete extraction of water bodies. Taking the water bodies in Xinyang City as an example, the overall accuracy of the confusion matrix was the highest, with an overall accuracy of 93.0% compared with the traditional index method. Combined with water bodies in Zhengzhou City for model validation, it has high applicability. Whether the water body is large or small, the extraction results are complete, with no voids or broken, and the accuracy is high.
Anterior atlantoaxial motion preservation fixation technique for axis complex fractures (odontoid process with Hangman’s fractures) and technique notes
Authors: Lu Qilin , Tang Jin , Xie Wei , Mei Xianzhong , Kang Hui , Liu Ximing , Xu Feng , Cai Xianhua 
Published: January 2024

AbstractThis study aims to investigate the feasibility and efficacy of anterior atlantoaxial motion preservation fixation (AMPF) in treating axis complex fractures involving the odontoid process fracture and Hangman’s fractures with C2/3 instability. A retrospective study was conducted on eight patients who underwent AMPF for axis complex fractures at the General Hospital of Central Theater Command from February 2004 to October 2021. The types of axis injuries, reasons for injuries, surgery time, intraoperative blood loss, spinal cord injury classification (American Spinal Injury Association, ASIA), as well as complications and technical notes, were documented. This study included eight cases of type II Hangman’s fracture, five cases of type II and three cases of type III odontoid process fracture. Five patients experienced traffic accidents, while three patients experienced falling injuries. All patients underwent AMPF surgery with an average intraoperative blood loss of 288.75 mL and a duration of 174.5 min. Two patients experienced dysphagia 1 month after surgery. The patients were followed up for an average of 15.63 months. One case improved from C to E in terms of neurological condition, three cases improved from D to E, and four cases remained at E. Bony fusion and Atlantoaxial Motion Preservation were successfully achieved for all eight patients. AMPF is a feasible and effective way for simultaneous odontoid process fracture and Hangman’s fractures with C2/3 instability, while preserving atlantoaxial movement.