bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vpliv nekaterih dejavnikov na naselitev navadne pršice (Tetranychus urticae Koch) na krizanteme Chrysanthemum 'Veria Dark ' in 'Cassablanca White'
Authors: REMIC Mojca , MILEVOJ Lea , PINTAR Marina 
Published: December 2023

Raziskava nekaterih dejavnikov (temperatura zraka, relativna zračna vlaga, načini namakanja in gostota nežleznih dlačic) v povezavi z naselitvijo navadne pršice (Tetranychus urticae Koch) na krizanteme Chrysanthemum 'Veria Dark' in 'Cassablanca White' je potekala v letih 2003 in 2004 v rastlinjaku in v laboratorijih na Biotehniški fakulteti (Ljubljana). Krizanteme obeh sort so bile vzgojene iz sadik s koreninsko grudico. Tehnologija gojenja krizantem je bila v skladu s priporočili stroke in na podlagi lastnih opazovanj. Namakanje je bilo leta 2003 kapljično in poplavno, leta 2004 pa samo poplavno. Na naselitev navadne pršice na preučevani sorti krizantem vpliva način naselitve in poraščenost listov z nežleznimi dlačicami. Na razvoj navadne pršice vplivata temperatura zraka in relativna zračna vlaga. Prevelika količina vode v substratu pa negativno vpliva na kondicijo krizantem.
Mutagenic treatment induces high transposon variation in barley (Hordeum vulgare L.)
Authors: POLOK Kornelia , ZIELINSKI Roman 
Published: December 2023

With more than 2700 mutant-derived cultivars, mutation techniques belong to the most efficient breeding methods. Despite a relatively good understanding of mutagenesis there is no agreement about the range of genome changes in mutants. Visualizing the gain and the loss of transposon insertion sites in SSAP profiles, the present studies aimed to answer whether or not mutants can be regarded as near isogenic lines with respect to their parent cultivar. Activities of BARE-1 retrotransposon and Tpo1-like DNA transposon from the CACTA superfamily were analysed in ten barley mutants derived by mutagenic treatment of two cultivars, Brenda and Scarlett. A large number and proportion of mutations (on average 21.4 mutations encompassing 19.7% loci) confirm high efficiency of applied mutagens. However, differences exist among both cultivars and transposons. The lack of clear correlation between transposon activities and morphology reflects different mechanisms shaping the mutant architecture. With respect to a transposon type, BARE-1 and Tpo1-like were equally active in inducing mutations. However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei’s similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. This way mutagenic treatment not only provides rough breeding materials but also can be used as a model in evolutionary studies.
Migration of Scaphoideus titanus Ball from the surrounding vineyards into the nursery
Authors: GRIŽON Primož , SELJAK Gabrijel , VUK Irma 
Published: December 2023

A one-year study of the mobility of leafhopper Scaphoideus titanus Ball (ST), vector of Flavescence dorée phytoplasma, was conducted in 2009 and included three Slovenian nurseries in the Primorska winegrowing region, as well as two Italian and one Swiss nursery, and was aiming at evaluating the risk of spreading ST with vine propagating material. The presence of ST was monitored by the means of yellow sticky traps that were placed at three distances in the nurseries and the nearby vineyards on three parallel lines (replicates). The traps were equidistantly placed from the nurseries’ and vineyards’ borders in order to determine the possible distance gradient. The monitoring started in the latest nymphal stages (middle of June) and terminated in the first decade of October. Insecticide treatments with deltamethrin (Decis 2.5 EC) were applied in nurseries, according to the instructions for use and recommendations for suppression of ST. On all Slovenian plots in the vineyards, the population level of ST was high (1796 adults in average / vineyard or 18 adults per trap / week), which indicated a great migration potential of the vector towards the nurseries. Catches were very low (54 adults in average / nursery or 0.4 adults / trap / week) on two nurseries’ plots (Lože and Slap), although high numbers of ST adults were caught in the adjacent untreated vineyards. On the Poreče nursery plot, insecticide treatments were not sufficient to avoid high numbers of ST catches (in total 481 adults or 3 adults / trap / week). This could be related to the sub-optimal timing of the first insecticide treatment and/or migration of adults from two nearby untreated vineyards located at a distance of 70 and 150 m. The distance between the nurseries and vineyards did not affect ST catches in the nurseries.
Expression and molecular analysis of DsRed and gfp fluorescent genes in tobacco (Nicotiana tabacum L.)
Authors: OVEN Kati , LUTHAR Zlata 
Published: December 2023

Agrobacterium-mediated transformation of tobacco leaf disks with Agrobacterium tumefaciens (A. t.) strain LBA4404 and two plasmids (pCAMBIA1390-DsRed and pART27 2mgfp5-ER) was used for introducing red fluorescent gene (DsRed), green fluorescent gene (gfp) and corresponding selection genes (hptII for resistance to antibiotic hygromycin and nptII for resistance to kanamycin) into leaf discs of tobacco (Nicotiana tabacum L.). Epifluorescent microscopy with the appropriate set of filters did not reveal phenotypic expression of the DsRed gene in 6.9 % of regenerants and the gfp gene in 1.3 % of regenerants that were successfully grown on selective medium. The duplex PCR method also did not confirm the presence of fragments specific to DsRed or gfp genes in these regenerants, while the presence of fragments characteristic of selection genes hptII and nptII was confirmed. A built-in nptII gene mutation, a deletion, was detected in one regenerant. Out of the 139 regenerants generated after the transformation of A. t.-pCAMBIA1390-DsRed, 38 or 25.5 % successfully grew only on non-selective medium; after transformation with A. t.-pART27 2mgfp5-ER 9 or 5.4 % of the 161 generants grew successfully. PCR analysis confirmed in all regenerants the presence of fragments characteristic of both transgenes, which were not expressed or were silenced. The effectiveness of transformation after infection with A. t.-pCAMBIA1390-DsRed was 93.1 %, and 98.7 % after infection with A. t.-pART27 2mgfp5-ER. We established that both fluorescent genes are suitable for setting up a transformation system. The antibiotics hygromycin and kanamycin successfully prevented the growth of untransformed tissues, but the antibiotic timentin successfully prevented the growth of bacteria A. t. after the transformation.
Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis
Authors: RADWAN Safaa A. , El-KOLY Aziza S. , SAMMOUR Reda H. 
Published: December 2023

Eighteen L. inconspicuous accessions collected from different countries were evaluated for variations of seed weight, seed protein content, and electrophoretic patterns of the total seed proteins analyzed under reducing conditions. They exhibited a reasonable genetic variability for the evaluated traits. This genetic variability revealed that improvement through simple selection for these traits is possible. The variation between the seed size of this accessions was attributed to the development process or the life cycle of the plant, and the environmental condition to which the mother plant is exposed. On the other hand, the variation in protein content among the different accessions may be due to genotype and/or seasonal influences. The relationship between protein content and 100 seeds weight in the evaluated accessions was reversible, the accession showed the lowest quantity of the total seed proteins was the accession that exhibited highest weight of 100 seeds and nearly vice versa. Each accession gave a different electrophoretic pattern except the two accessions collected from Iran, exhibited an identical one. The difference in 100 seed weight and total protein content of these accessions indicated that they are not genetically identical. The variation in the electrophoregram of the evaluated accessions located in the bands with molecular weight more than 98 kDa, the heavy subunits of alpha-lathyrin subunits and the region molecular weight around 70 kDa. The results of cluster analysis based of SDS/PAGE under reduction conditions indicated that genetic diversity between Turkish, Syrian, and Iranian and Australian accessions is pronounced, and Turkish accessions are closer to both Syrian and Iranian accessions than the relation between Syrian and Iranian. This suggested that crosses between the Iranian and Syrian accessions could create more genetic variability than crosses with Turkish accessions. The distribution of Turkish and Syrian accessions between more than one clusters revealed that genetic diversity and geographic distribution were independent of each other. PCA showed that all accessions were separated on the first principal component, indicating that the accessions showed a good association, due, probably, to parallel evolution.
Effect of intermittent irrigation with saline water on rice yield in Rasht, Iran
Authors: REZAEI M. , DAVATGAR N. , KHALEDIAN M.R. , PIRMORADIAN N. 
Published: December 2023

Guilan, a well-known province in rice production in Iran, has been facing water shortage and water degradation. In order to study the effects of salinity stress as well as water stress on rice a pot experiment was conducted at Rice Research Institute of Iran. Five water salinity levels: fresh water (EC = 1 dS m-1), 2, 4, 6 and 8 dS m-1 and five irrigation regimes: continues flooding, Alternative Wetting and Drying (AWD), intermittent irrigation at 100, 90 and 80 percent of field capacity (FC) were considered as irrigation treatments. The results showed severe effects of water and salinity stresses on rice yield and yield components. Fresh water produced the highest yield, 18.57 gr pot-1, whereas, the yield in salinity levels of 2, 4, 6 and 8 dS m-1 were 13.78, 5.78, 3.61 and 0.74 gr pot-1, respectively, with the yield losses of 25, 70, 80 and 97%, respectively. Intermittent irrigation at FC produced the highest yield. The yield increased 8 and 13% in AWD and intermittent irrigation at FC treatments respectively, while it decreased 8 and 27% in intermittent irrigation at 80 and 90% of FC treatments as compared with continues flooding treatment. The highest yield with application of intermittent irrigation at FC was valid only in water salinity less than 4 dS m-1. When water salinity was higher than 4 dS m-1 all irrigation methods gave the same yield. This study showed that the best method to use saline water was intermittent irrigation at FC with EC = 2 dS m-1. In case of more salinity, mixing fresh and saline water and intermittent irrigation can mitigate the severe effects of salinity on rice.
Antioksidativni potencial otrobov pire, navadne in tatarske ajde
Authors: LUKŠIČ Lea 
Published: December 2023

Pira, navadna in tatarska ajda vsebujejo antioksidante in še nekatere snovi, ugodne za ohranjanje zdravja ljudi. V raziskovalni nalogi smo preučevali predvsem koliko in katere antioksidante vsebujejo otrobi posameznih vrst rastlin in v kakšnem zaporedju po količini antioksidantov, si vrste sledijo. Antioksidante smo tako v vzorcih rastlin določali spektrofotometrično. Ugotovili smo, da otrobi tatarske ajde vsebuje največ antioksidantov. Njena skupna antioksidativna aktivnost znaša 87,23 % DPPH razbarvanja, sledi ji navadna ajda z 11,71 % DPPH razbarvanja, najmanjšo skupno antioksidativno aktivnost pa ima pira z 1,01 % DPPH razbarvanja.
Novejši podatki o vsebnosti semen vrst iz rodu Ambrosia v krmi za prostoživeče ptice v Sloveniji
Authors: JAKOVAC STRAJN Breda , POZVEK Kristina Jelka , PROSENIK Tanja , LEŠNIK Mario , UJČIČ VRHOVNIK Igor 
Published: December 2023

Vdihavanje peloda vrst iz rodu Ambrosia lahko povzroči preobčutljivostne reakcije. Krma za prostoživeče ptice je eden od dejavnikov, ki pripomorejo k širjenju omenjenih rastlin. Leta 2010 so zato k Direktivi o nezaželenih snoveh v živalski krmi (2002/32/ES) dodali aneks, da lahko krma za živali, ki vsebuje nezmleta žita, vsebuje do 50 mg semen vrst iz rodu Ambrosia v kilogramu krme (UL L 290/54). Podatkov o vsebnosti semen te rastline v krmi je zelo malo, zato smo z mikroskopsko metodo preiskali 40 vzorcev krme za prostoživeče ptice. Semena ambrozije je vsebovalo 20 vzorcev oziroma 50 %. Ugotovljeno število semen v kilogramu posameznega vzorca je bilo od 2 do 146 (10 mg do 774 mg). V skladu s predpisi smo vsebnosti preračunali relativno na vzorec z 12 % vlage in ugotovili, da je dovoljeno mejo presegalo 5 vzorcev (12,5 %).
Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP
Authors: CIZELJ Ivanka , BERČIČ Rebeka Lucijana , DUŠANIĆ Daliborka , BENČINA Mateja , NARAT Mojca , ZORMAN-ROJS Olga , BENČINA Dušan 
Published: December 2023

Major poultry pathogens, Mycoplasma gallisepticum and Mycoplasma synoviae possess cysteine protease CysP, which in vitro cleaves chicken immunoglobulin G (IgG) into Fab (antigen-binding fragment) and Fc (crystallisable region fragment). We used recombinant CysP of M. synoviae (rCysP) in enzyme immunoassays to detect antibodies to CysP in sera, synovial fluids, and in washings of respiratory tract and oviducts of chickens and turkeys experimentally or naturally infected with M. gallisepticum or M. synoviae. In poultry infected with M. synoviae, 70.4 % of samples contained antibodies reacting with rCysP. In birds infected with M. gallisepticum we detected CysP antibodies in 63.1 % of samples. Our data demonstrate that CysP is immunogenic for chickens and turkeys and indicate that M. gallisepticum and M. synoviae infecting chickens and turkeys synthesise CysP in vivo. This is the first study demonstrating that proteases of any Mycoplasma species can induce production of specific antibodies in the natural host.
effect of uncertainty in a fuzzy Input-Output analysis of water consumption applied to Macedonia
Authors: HRISTOV Jordan , SURRY Yves 
Published: December 2023

This paper reconsiders the reliability of the results obtained by Hristov et al. (2012) from the input-output analysis of water consumption, by taking into account the effect of uncertainty inherent in the data. The imprecision and uncertainty is studied within a workable fuzzy environment as introduced by Beynon et al. (2005), where rank reversal is plausible. In addition, the water-intensive structure of the Macedonian economy is investigated by applying Dietzenbacher eigenvector methodology (1992). As suggested in the analysis of Hristov et al. (2012), the water-intensive structure of the economy in Macedonia is confirmed as being mainly focused on agriculture and several industrial sectors, given that rank reversal is absent. Consequently, there is a need to introduce changes in the agricultural production technology as well as the specialization of production in this region.
Cervical length as a screening tool for preterm birth in twin pregnancies: a systematic review and critical evaluation of quality clinical practice guidelines
Authors: De Vito Marika , Cetraro Jessica , Capannolo Giulia , Alameddine Sara , Patelli Chiara , D’Antonio Francesco , Rizzo Giuseppe 
Published: December 2023

Abstract

Objectives
Twin pregnancies are at increased risk of preterm birth (PTB) compared to singletons. Evaluation of cervical length (CL) represents the optimal tool to screen PTB in singleton. Conversely, there is less evidence on the use of CL in twins. Our aim was to evaluate the methodological quality and clinical heterogeneity of clinical practice guidelines (CPGs) on the CL application in twins using AGREE II methodology.

Methods
MEDLINE, Scopus, and websites of the main scientific societies were examined. The following aspects were evaluated: diagnostic accuracy of CL, optimal gestational age at assessment and interventions in twin pregnancies with reduced CL. The quality of the published CPGs was carried out using “The Appraisal of Guidelines for REsearch and Evaluation (AGREE II)” tool. The quality of guideline was rated using a scoring system. Each considered item was evaluated by the reviewers on a seven-point scale that ranges from 1 (strongly disagree) to 7 (strongly agree). A cut-off >60 % identifies a CPGs as recommended.

Results
The AGREE II standardized domain scores for the first overall assessment had a mean of 74 %. The score was more than 60 % in the 66.6 % of CPGs analyzed indicating an agreement between the reviewers on recommending the use of these CPGs. A significant heterogeneity was found; there was no specific recommendation on CL assessment in about half of the published CPGs. There was also significant heterogeneity on the CL cut-off to prompt intervention.

Conclusions
Despite the fact that the AGREE II analysis showed that the majority of the included guidelines are of good quality, there was a significant heterogeneity among CPGs as regard as the indication, timing, and cut-off of CL in twins as well as in the indication of interventions.
On the Structure Lie Operator of a Real Hypersurface in the Complex Quadric
Authors: De Dios Pérez Juan , Pérez-López David , Suh Young Jin 
Published: December 2023

ABSTRACT
The almost contact metric structure that we have on a real hypersurface M in the complex quadric Qm
= SO

m+2/SO

m

SO
2 allows us to define, for any nonnull real number k, the k-th generalized Tanaka-Webster connection on M,

∇
^

(
k
)

. Associated to this connection, we have Cho and torsion operators

F
X

(
k
)

and

T
X

(
k
)

, respectively, for any vector field X tangent to M. From them and for any symmetric operator B on M, we can consider two tensor fields of type (1,2) on M that we denote by

B
F

(
k
)

and

B
T

(
k
)

, respectively. We classify real hypersurfaces M in Qm
for which any of those tensors identically vanishes, in the particular case of B being the structure Lie operator Lξ
on M.
The Alpha Power Muth-G Distributions and Its Applications in Survival and Reliability Analyses
Authors: Eghwerido Joseph Thomas , Agu Ikechukwu Friday 
Published: December 2023

ABSTRACT
The generalization of the family of distributions that could provide a simple, and efficient algorithm for parameter estimation and study of the behavior of datasets from various fields has received significant interest. Such a model has enormous advantages, such as its flexible nature, and the regression form can easily be derived. In the literature, various generalized families of distributions have been introduced. Despite the merits of these distributions, they still have some limitations due to many parameters in the model. Thus, the estimation of parameters often becomes cumbersome. Therefore, this study introduced the alpha power Muth or Teissier-G family of continuous distributions with well-defined parameters, and obtained the joint progressive type-II censoring scheme and their reliability measures. Furthermore, we obtained the global and local influences of the APTG model. We used real-life and simulated data to evaluate the numerical applications of the introduced model. The results show that the alpha power Muth or Teissier-G family of distributions gave the best fits to both datasets than some existing models.
Social partners and OSH: a multilevel and cross-country exploration in the hospital and social services sectors
Authors: Weber Sabrina , Bechter Barbara , Galetto Manuela , Guobaite Ramune , Blaziene Inga , Hiltunen Linda , Larsson Bengt , Pankow Maciej , Czarzasty Jan , Owczarek Dominik 
Published: December 2023

Abstract
The article examines the role of social partners (trade unions and employers) in the field of occupational safety and health (OSH). The Covid-19 pandemic directed attention to the importance of greater national and European level policy coordination to protect and promote healthy, safe, and well-adapted work environments. On the basis of two sectors, hospitals and social services with a focus on elder care, the article asks how OSH policy interventions at the national level and the European level are interlinked. To explore interlinkages, the article focuses on OSH policy coordination between national social partners and European level sectoral social dialogue. The analysis is informed by actor-centred institutionalist and multilevel governance approaches and uses qualitative data. The article explores patterns of interlinkages between the national and European levels in two sectors and six countries and discusses the limitations of applying a cross-national and multilevel conceptual approach. The analysis shows that the pandemic has promoted some coordination at national and between national and EU levels, yet still rather modest interlinkages and degrees of ‘vertical’ coordination can be observed. The paper concludes that there continues to be a degree of ‘in-the-making’ to the multilevel governance of employment relations, even in the presence of common challenges.
Equity and local health systems: a qualitative evaluation of the experiences of local health service leads during the first two years of the NHS Low Calorie Diet programme pilot
Authors: Drew Kevin , Homer Catherine , Radley Duncan , Freeman Charlotte , Kinsella Karina , Maynard Maria , Bakhai Chirag , Ells Louisa 
Published: December 2023

Background: Obesity and type 2 diabetes (T2DM) can both profoundly impact health and wellbeing. Their prevalence largely follows a social gradient. The National Health Service Low Calorie Diet programme in England aims to support people to achieve T2DM remission while also reducing health inequalities. We aimed to explore the experiences of local health service leads and identify barriers and facilitators in relation to the equitable mobilisation of the Low Calorie Diet programme.
Methods: Twenty semi-structured interviews were completed with 24 locality leads across the first two years of the Low Calorie Diet programme. Interviewees were purposively sampled from the 10 localities who undertook the Low Calorie Diet programme pilot. Each interview explored a number of topics of interest, including referrals, training, communication, incentivisation, governance and engagement, before being subjected to a thematic analysis.
Results: From the data, seven core themes were identified: COVID-19 and primary care capacity and engagement; methods of communication; approaches to training; approaches to incentivisation; approaches to referrals; barriers to referrals; and the importance of collaboration. COVID-19 presented a specific challenge to the mobilisation and delivery of the Low Calorie Diet programme; however, our findings demonstrate the large variation and differences in the approaches taken when delivering the programme across 10 geographically and demographically distinct pilot sites. We also identified a lack of a recognised approach or strategy to mobilisation and delivery support for the Low Calorie Diet programme, such as proportionate universalism, which is a social policy response to tackling health inequalities by ensuring that service delivery is equitable.
Conclusions: Health inequalities remain a significant challenge, and health service leads have the potential to adopt an equity perspective from the start of programme mobilisation. In doing so, resources at their disposal can be managed equitably and can therefore contribute to efforts to reduce the potential occurrence of intervention-generated inequalities.