bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile
Authors: Yang Xue-Ting , Wang Jie , Jiang Ying-Hua , Zhang Lei , Du Ling , Li Jun , Liu Feng 
Published: December 2023

IntroductionCholesterol gallstone disease is a prevalent condition that has a significant economic impact. However, the role of the bile microbiome in its development and the host’s responses to it remain poorly understood.MethodsIn this study, we conducted a comprehensive analysis of microbial and human bile proteins in 40 individuals with either gallstone disease or gallbladder polyps. We employed a combined proteomic and metaproteomic approach, as well as meta-taxonomic analysis, functional pathway enrichment, and Western blot analyses.ResultsOur metaproteomic analysis, utilizing the lowest common ancestor algorithm, identified 158 microbial taxa in the bile samples. We discovered microbial taxa that may contribute to gallstone formation, including β-glucuronidase-producing bacteria such as Streptococcus, Staphylococcus, and Clostridium, as well as those involved in biofilm formation like Helicobacter, Cyanobacteria, Pseudomonas, Escherichia coli, and Clostridium. Furthermore, we identified 2,749 human proteins and 87 microbial proteins with a protein false discovery rate (FDR) of 1% and at least 2 distinct peptides. Among these proteins, we found microbial proteins crucial to biofilm formation, such as QDR3, ompA, ndk, pstS, nanA, pfIB, and dnaK. Notably, QDR3 showed a gradual upregulation from chronic to acute cholesterol gallstone disease when compared to polyp samples. Additionally, we discovered other microbial proteins that enhance bacterial virulence and gallstone formation by counteracting host oxidative stress, including sodB, katG, rbr, htrA, and ahpC. We also identified microbial proteins like lepA, rtxA, pckA, tuf, and tpiA that are linked to bacterial virulence and potential gallstone formation, with lepA being upregulated in gallstone bile compared to polyp bile. Furthermore, our analysis of the host proteome in gallstone bile revealed enhanced inflammatory molecular profiles, including innate immune molecules against microbial infections. Gallstone bile exhibited overrepresented pathways related to blood coagulation, folate metabolism, and the IL-17 pathway. However, we observed suppressed metabolic activities, particularly catabolic metabolism and transport activities, in gallstone bile compared to polyp bile. Notably, acute cholelithiasis bile demonstrated significantly impaired metabolic activities compared to chronic cholelithiasis bile.ConclusionOur study provides a comprehensive metaproteomic analysis of bile samples related to gallstone disease, offering new insights into the microbiome-host interaction and gallstone formation mechanism.
Serum ammonia variation predicts mortality in patients with hepatitis B virus-related acute-on-chronic liver failure
Authors: Cai Yi-Jing , Dong Jia-Jia , Chen Rui-Cong , Xiao Qian-Qian , Li Xu-Mei , Chen De-Yuan , Cai Chao , Lin Xiu-Li , Shi Ke-Qing , Lu Ming-Qin 
Published: December 2023

BackgroundHyperammonemia is critical to the development of hepatic encephalopathy (HE) and is associated with mortality in end-stage liver disease. This study investigated the clinical value of ammonia variation in hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) patients.MethodsA total of 276 patients with HBV-ACLF were retrospectively recruited. Patients' ammonia levels were serially documented. Baseline ammonia, Peak ammonia (highest level), and Trough ammonia (lowest level) were particularly corrected to the upper limit of normal (AMM-ULN). The primary endpoint was 28-day mortality.ResultsThe 28-day, 3-month, and 12-month mortality rates were 19.2, 25.7, and 28.2%, respectively. A total of 51 (18.4%) patients had overt HE (grade 2/3/4). Peak AMM-ULN was significantly higher in patients with overt HE and non-survivors compared with their counterparts (P &lt; 0.001). Following adjustment for significant confounders, high Peak AMM-ULN was an independent predictor of overt HE (hazard ratio, 1.031, P &lt; 0.001) and 28-day mortality (hazard ratio, 1.026, P &lt; 0.001). The cut-off of Peak AMM-ULN was 1.8, determined by using the X-tile. Patients with Peak AMM-ULN appearing on days 1–3 after admission had a higher proportion of overt HE and mortality compared to other groups. Patients with decreased ammonia levels within 7 days had better clinical outcomes than those with increased ammonia.ConclusionSerum Peak ammonia was independently associated with overt HE and mortality in HBV-ACLF patients. Serial serum ammonia may have prognostic value.
Evaluation of the shear bond strength of a tricalcium silicate-based material to four self-adhering glass ionomer materials: an in vitro study
Authors: BinSaleh Saad , Sulimany Ayman M. , Aldowsari Mannaa K. , Al-Homaidhi Majedah , Alkuait Nour , Almashham Lama , Alghamdi Nada 
Published: December 2023

BackgroundThis study aimed to evaluate and compare the shear bond strength (SBS) of EQUIA Forte HT with that of other restorative materials, including EQUIA Forte, glass ionomer cement (GIC), and resin-modified glass ionomer cement (RMGIC) when bonded to NeoMTA 2.Materials and methodsA total of 120 holes were created in Teflon molds and filled with NeoMTA 2. The restorative materials were immediately applied using customized silicone molds. The samples were randomly divided into two main groups: one to measure the immediate SBS and the other to measure the delayed SBS. These two main groups were further divided into four subgroups based on the restorative material used: EQUIA Forte HT, EQUIA Forte, GIC, and RMGIC.ResultsThe study groups showed statistically significant differences in the mean SBS (p &lt; 0.0001). The immediate SBS of the RMGIC group (mean ± SD: 5.43 ± 1.22) was significantly higher than those of the GIC and EQUIA Forte groups, with no significant difference found compared to the SBS of EQUIA Forte HT. In the delayed SBS, both the RMGIC and EQUIA Forte HT groups (4.98 ± 0.67 and 4.93 ± 0.60, respectively) demonstrated significantly higher bond strengths than the GIC and EQUIA Forte groups (3.81 ± 0.57 and 4.2 ± 0.63, respectively). However, there were no statistically significant differences between the RMGIC and EQUIA Forte HT groups or between the GIC and EQUIA Forte groups.ConclusionBased on our findings, EQUIA Forte HT has shown promising outcomes when used as a restorative material following pulpotomies, with results comparable to those of RMGIC.
Imperatorin ameliorates pulmonary fibrosis via GDF15 expression
Authors: Tsai Cheng-Fang , Chen Yen-Chang , Li Ya-Zhen , Wu Chen-Teng , Chang Pei-Chun , Yeh Wei-Lan 
Published: December 2023

Background: Pulmonary fibrosis features in damaged pulmonary structure or over-produced extracellular matrix and impaired lung function, leading to respiratory failure and eventually death. Fibrotic lungs are characterized by the secretion of pro-fibrotic factors, transformation of fibroblasts to myofibroblasts, and accumulation of matrix proteins.Hypothesis/purpose: Imperatorin shows anti-inflammatory effects on alveolar macrophages against acute lung injury. We attempt to evaluate the properties of imperatorin on the basis of fibroblasts.Methods: In in vitro, zymosan was introduced to provoke pro-fibrotic responses in NIH/3T3 or MRC-5 pulmonary fibroblasts. Imperatorin was given for examining its effects against fibrosis. The mice were stimulated by bleomycin, and imperatorin was administered to evaluate the prophylactic potential in vivo.Results: The upregulated expression of connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), and collagen protein due to zymosan introduction was decreased by imperatorin in fibroblasts. Zymosan induced the activity of transglutaminase 2 (TGase2) and lysyl oxidase (LOX), which was also inhibited by the administration of imperatorin. Imperatorin alone enhanced sirtuin 1 (SIRT1) activity and growth differentiation factor 15 (GDF15) secretion in fibroblasts via LKB1/AMPK/CREB pathways. In addition, GDF15 exerted a beneficial effect by reducing the protein expression of CTGF, α-SMA, and collagen and the activities of TGase and LOX. Moreover, orally administered imperatorin showed prophylactic effects on bleomycin-induced pulmonary fibrosis in mice.Conclusion: Imperatorin reduces fibrotic marker expression in fibroblasts and also increases GDF15 secretion via the LKB1/AMPK/CREB pathway, attenuating pro-fibrotic responses in vitro. Imperatorin also alleviates pulmonary fibrosis induced by bleomycin in vivo.
A review of factors influencing sensitive skin: an emphasis on built environment characteristics
Authors: Chen Xiangfeng , Wen Jing , Wu Wenjuan , Peng Qiuzhi , Cui Xiangfen , He Li 
Published: December 2023

BackgroundSensitive skin (SS) is a condition characterized by hyperreactivity. Impacting around 37 percent of the worldwide population and exerting an influence on the quality of life for affected individuals. Its prevalence rate has increased due to factors such as elevating stress levels and deteriorating environmental conditions. The exposome factors influencing SS have extended from demographic, biological attributes, and lifestyle to external environments. Built environments (BEs) have demonstrated as root drivers for changes in behaviors and environmental exposure which have the potential to trigger SS, but the review of the associations between BEs and SS is currently lacking.ObjectiveThis review aims to achieve two primary objectives: (1) Examine exposome factors that exert influence on SS at the individual and environmental levels. (2) Develop a theoretical framework that establishes a connection between BEs and SS, thereby offering valuable insights into the impact of the built environment on this condition.MethodsAn extensive literature search was carried out across multiple fields, including sociology, epidemiology, basic medicine, clinical medicine, and environmental research, with a focus on SS. To identify pertinent references, renowned databases such as PubMed, Web of Science, and CNKI were utilized.ResultsSS is the outcome of interactions between individual attributes and environmental factors. These influencing factors can be categorized into five distinct classes: (1) demographic and socioeconomic characteristics including age, gender, and race; (2) physiological and biological attributes such as emotional changes, skin types, sleep disorders, and menstrual cycles in women; (3) behavioral factors, such as spicy diet, cosmetic use, alcohol consumption, and physical exercise; (4) natural environmental features, including climate conditions and air pollution; (5) built environmental features such as population density, green space availability, road network density, and access to public transportation, also have the potential to affect the condition.ConclusionThe importance of interdisciplinary integration lies in its ability to ascertain whether and how BEs are impacting SS. By elucidating the role of BEs in conjunction with other factors in the onset of SS, we can provide guidance for future research endeavors and the formulation of interventions aimed at mitigating the prevalence of SS.
Effects of Gamma Irradiation on the AC Electrical Properties of Cross-Linked Epoxy Resin/Bisphenol A-Based Polycarbonate Composites
Authors: Alqudah Ziad , Juwhari Hassan K. , Elimat Ziad 
Published: December 2023

The effects of gamma radiation on the AC electrical properties of highly cross-linked epoxy resin/bisphenol A-based polycarbonate samples have been investigated as a function of concentrations of bisphenol A-based polycarbonate, frequency, and temperature. The composite samples contained different bisphenol A-based polycarbonate concentrations of 0, 4, 8, 10, and 15 by wt%. The gamma irradiation process was performed at different gamma doses of 0, 100, 300, and 500 Gy. The AC electrical properties of the tested samples were studied before and after gamma irradiation within a frequency range of 200 kHz to 1 MHz. The results show that after irradiation, a consistent decrease in complex impedance values (Z∗) was observed, indicating an increase in conductivity due to radiation-induced scission of the composite structure. Dielectric properties, including the dielectric constant (εr) and dielectric loss (εi), exhibited an increase with higher doses and higher polycarbonate concentrations, signifying the formation of defect sites and charge carrier trapping. AC electrical conductivity (σac) displayed a notable rise post irradiation, with temperatures ranging from 30 °C to 110 °C, and higher radiation doses and higher temperatures led to increased conductivity. The activation energy ( Ea) decreased as the radiation dose increased, reflecting structural modifications induced by radiation.
Genome-Wide Identification and Analysis of ZF-HD Gene Family in Moso Bamboo (Phyllostachys edulis)
Authors: Huang Feiyi , Wang Jiaxin , Tang Chao 
Published: December 2023

Zinc finger-homeodomain (ZF-HD) proteins play essential roles in plant growth, development and stress responses. However, knowledge of the expression and evolutionary history of ZF-HD genes in moso bamboo remains limited. In this study, a total of 24 ZF-HD genes were found unevenly distributed on 12 chromosomes in moso bamboo (Phyllostachys edulis). Phylogenetic analysis indicated that PeZF-HDs were divided into two subfamilies: ZHD and MIF. The ZHD subfamily genes were further classified into seven groups according to their orthologous relationships among the rice and Arabidopsis ZF-HD gene family. The gene structures and conserved motifs of PeZF-HDs were analyzed. Whole-genome duplication (WGD) or segmental duplication promoted the evolution and expansion of the moso bamboo ZF-HD gene family. Ka/Ks ratios suggested that the twenty-four duplication pairs had undergone purifying selection. Promoter analysis showed that most PeZF-HDs contained cis-elements associated with stress responses and hormones. Expression analysis demonstrated that many PeZF-HDs were responsive to abiotic stress treatment. Overall, this work investigated PeZF-HD genes in moso bamboo using bioinformatic approaches. The evolutionary research on gene structure, motif distribution and cis-regulatory elements indicated that PeZF-HDs play distinct roles in biological processes, which provides a theoretical basis for exploring the physiological functions of ZF-HDs and selecting candidate stress-related genes in moso bamboo.
MODEL OF THE MOBILE ROBOTIC COMPLEX MANAGEMENT SYSTEM
Authors: Березкин А.А. , Вивчарь Р.М. , Киричек Р.В. 
Published: December 2023

В статье поставлена задача по разработке модели функционирования системы управления мобильным роботизированным комплексом и приведено ее формальное описание. Представлена структура модели, описаны ее основные элементы. Определена система показателей эффективности системы управления роботизированными комплексом. Описан подход к обоснованию требований к показателям эффективности и их рассчитанные требуемые значения.
The article presents the task of developing a model for the operation of a control system for a mobile robotic complex. Its formal description is given. The structure of the model is presented, its main elements are described. The system of performance indicators of the robotic complex control system is defined. An approach to substantiating the requirements for performance indicators and their calculated required values are described.
Pengaruh Lingkungan Kerja dan Insentif terhadap Kepuasan Kerja Karyawan UD. Trubus Kota Bekasi
Authors: Viona Rezkiani  , Amanda Setiorini  
Published: December 2023

The purpose of this study was to analyze the effect of the work environment and incentives on UD job satisfaction. Bekasi City Trubus. This research uses quantitative descriptive methods. The population of this study was all UD employees. Trubus has 510 employees. The sample of this study amounted to 60 employees. The sampling technique is with the formula isaac and michael. The data collection technique is carried out by questionnaires. The data analysis techniques used are multiple and part partially ial linear regression analysis, The results of this study show: First, there is a positive and significant influence of the work environment on job satisfaction. Second, there isa positive influence of incentives on job satisfaction. Third, there is a positive influence of the work environment and incentives on job satisfaction. The results of this study are expected to be useful for increasing job satisfaction through a comfortable work environment and incentives provided.
Separate evaluation of interface and oxide hole traps in SiO2/GaN MOS structures with below- and above-gap light excitation
Authors: Kobayashi Takuma , Tomigahara Kazuki , Nozaki Mikito , Shimura Takayoshi , Watanabe Heiji 
Published: December 2023

Abstract
Understanding the traps in metal-oxide-semiconductor (MOS) structures is crucial in the fabrication of MOS transistors with high performance and reliability. In this study, we evaluated the hole traps in SiO2/GaN MOS structures through photo-assisted capacitance-voltage measurements. Below- and above-gap light was used to distinguish between the contributions of fast interface and slow oxide hole traps. While annealing in oxygen is effective in reducing the oxide hole traps, a high density of hole traps exceeding 1012 cm−2eV−1 remains at the interface. Although these traps are donor-type and thus hidden in n-type MOS structures, they could impair the switching performance of GaN MOS transistors.
Influence Of Product Brand, Customer Satisfaction, Product Quality, Promotion, Service And Samsung Prices On Interest In Buying At Chandra's Shop Cellular Kotapinang
Authors: Triana Lia , Nasution Siti Lam'ah , Harahap Aziddin 
Published: December 2023

This study aims to investigate the effect of Brand Product, Custemer Satisfaction Product Quality, Promotion, Service And Samsung Prices On Purchase Interest In Chandra Celluler Shops. Data collection was carried out by distributing questionnaires and distributing them to 100 research samples, namely Chandra cellular consumers. Data analysis in this study used SPSS version 20. Data testing techniques were carried out in partial and simultaneous testing. The results of the analysis show that overall the independent variables have a positive and significant influence on the dependent variable. This can be seen from the value of all t counts which have a value higher than 1.985 with a significant value below 0.05. Overall the final result of the study is that the independent variables provide influence of 0.906 or 90.06% and the rest is influenced by other supporting factors.
The Effect of Service Quality and Clinic Image on Satisfaction to Mediate Revisit Intention and Loyalty in Beauty Clinic Industry
Authors: Lienata Andrew , Pink Berlianto Margaretha 
Published: December 2023

The aesthetic sector of healthcare services has grown significantly for both men and women in Indonesia and around the world. This study targets to examine the direct effect of service quality, which consists of tangibility, reliability, responsiveness, assurance, empathy, and clinic image, on patient satisfaction, which is assessed by the expectation of intention to revisit and patient loyalty. A questionnaire was used to collect data for this quantitative analysis. The research focused on patients who had received aesthetic treatment at SKYN Clinic in South Jakarta, Indonesia. According to data, there were 4,290 outpatients between January and August of 2022. It was determined that there were a total of 231 samples. The sampling technique used was systematic sampling. The research study utilized SEM-PLS (Partial Least Square-Structural Equation Modeling). Patient satisfaction positively influenced patient loyalty and revisit intention, while service quality and clinic image positively influenced patient satisfaction. This study revealed that to compete with the competitors in the beauty clinic industry, revisit intention and patient loyalty must be strengthened. Practitioners in the business of aesthetics, is required to rise customer satisfaction by promoting healthcare service quality factors and the overall clinic image.
A Strategies for Preventing and Handling Child Violence Through Assertive Training Methods in Banyuwangi Regency
Authors: Fitria Yuli , Wirawati Wahyu Adri , Rahman Machria 
Published: December 2023

Child violent abuses were fatally unrevealed because of the children’s inability to report and they do not even realize that they become victims of the abuse. Assertive training is expected to be an alternative method that can be used to improve children’s communicating their thoughts and feelings. Therefore, it needs to be proven through research. The objective of this study is to test the effectiveness of assertive training as a method to prevent and handle violence against children and improving children’s social skills in the form of assertive abilities. The study is a mixed method approaches and used purposive sampling technique. The sample were 100 students at two public junior high schools in Banyuwangi. For data analysis, the study used Paired Sample T- Test. The results of research showed assertive training had positive impact and was proven significantly increasing the assertive abilities. From the provision of assertive training, the children’s improved social skills were courage in expressing feelings, increased self-esteem, optimism and resilience. It can be concluded that the assertive training is able to increase assertive abilities, thus it can be an alternative method to prevent and handle violence against children.

Keywords: Assertive, training, violence, children
Analysis of the Effect of Financial Inclusion on the Success of MSME Business Rujak Traders in Natsepa Beach, Central Maluku Regency, Moderated by Fintech
Authors: Siaila Steven , Soukotta Agnes , De Fretes Anna F. 
Published: December 2023

This paper aims to test whether financial inclusion affects the success of the business of rojak merchant MSMEs in Natsepa Beach, Central Maluku Regency? Does Fintech affect the success of the business of rojak merchant MSMEs in Natsepa Beach? Does financial inclusion affect the success of the business of rojak merchant MSMEs in Natsepa Beach, Central Maluku Regency with Financial Technology as moderation? The samples taken were all rojak traders located at the location of Natsepa beach; The analysis technique used is path analysis (Path Analysis)
The results showed that Financial Inclusion had a positive and significant effect on the success of the rojak trader business in Natsepa Beach, Central Maluku Regency. Financial Technology (FINTECH) does not have a significant effect on the success of the rojak trader business on Natsepa beach, Central Maluku Regency. Financial Inclusion does not have a significant effect on the business success of rojak traders on Natsepa beach, Central Maluku Regency, mediated by FINTECH.