bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrasound imaging of the phrenic nerve at the scalene muscle level
Authors: Bedewi Mohamed Abdelmohsen , Habib Yomna S. , Sifey Ayman A. , Kotb Mamdouh Ali , Almalki Daifallah Mohammed , AlAseeri Ali Abdullah , Alhariqi Bader A. , Sandougah Kholoud J. , Alfaifi Tariq , Marsico Salvatore , Awad Mariam EM , Soliman Steven B. 
Published: October 2023

The aim of this study is to utilize ultrasound to evaluate the normal cross-sectional area (CSA) of the phrenic nerve (PN), at the level of the anterior scalene muscle. The study included 62 PNs in 31 healthy subjects (13 men, 18 women); mean age, 36.6 years; mean height, 161.1 cm; mean weight, 69.6 kg; and mean body mass index 25.8 kg/m2. High-resolution ultrasound images of the bilateral PNs were obtained by a radiologist with 15 years of experience in neuromusculoskeletal ultrasound. Three separate CSA measurements for the bilateral PNs bilaterally were obtained. Images were also reviewed by an experienced neurologist to evaluate for inter-rater variability. The mean CSA of the right PN was 0.54 mm2 ± 0.16. The mean CSA of the left PN was 0.53 mm2 ± 0.18. We believe that the reference values for the normal CSA of the PNs obtained in our study could help in the sonographic evaluation of PN enlargement, as it relates to the diagnosis of various diseases affecting the PN. Furthermore, knowledge of its location and size, at the level of the scalene muscle, could help prevent PN-related complications during interventional procedures in that area.
Additionally, for each participant, demographic information of age and gender as well as body mass index, weight, and height were documented.
Effectiveness of rational emotive behavior education (REBE) on computer anxiety among school teachers enrolled in e-learning
Authors: Onah Benardine Ifeoma , Ede Moses Onyemaechi , Eze Blessing Ngozi , Uzoegwu Chigozie Roseann , Mgboji Chukwuma , Agbo George Chibuike , Uzuagu Anthonia Uju , Chiamagu Charles , Agbo Philomina A , Oneli Joy Obiageli , Ogbueghu Sylvester N. 
Published: October 2023

Background:
Reports from past studies indicated that research on anxiety disorder is not extensive and further investigation is needed using different populations. Besides, psychological intervention for computer anxiety has not been given the required empirical attention. Given this, we investigated the effectiveness of rational emotive behavior education on computer anxiety among primary school teachers enrolled in e-learning.

Methods:
A pure experimental design was used, which helped to randomly assigned recruited 112 primary school teachers to 2 arms (treatment and control groups). The Computer Anxiety Scale was distributed before, immediately after, and 4 weeks after completion of intervention with 12 sessions that were given to the treatment group.

Results:
The ANOVA results showed that rational emotive behavior education significantly decreased teachers computer anxiety. The mean computer anxiety score among instructors did not significantly differ by group or gender. Therefore, we recommended that computer educators, policymakers, and rational emotive behavior educators should organize rational and emotive-oriented conferences for schoolteachers.
Could BMMNCs therapy reduce the mid- and long-term rate of total hip arthroplasty of femoral head necrosis?: A systematic review and meta-analysis
Authors: Chen Xiao , Chen Jing , Duan Yanji , Chen Chang , Cao Yuan 
Published: October 2023

Background:
Osteonecrosis of the femoral head (ONFH) is a cause of hip pain and early joint lesion in patient. The hip-preserving treatments are especially important for patients in early stage of ONFH. However, it is controversial of the effectiveness and safety of bone marrow mononuclear cells (BMMNCs) in the treatment of ONFH. The aim of the study was to explore the mid- and long-term efficacy (particularly the rate of total hip arthroplasty [THA]) with BMMNCs in treatment of ONFH.

Methods:
PubMed, Web of Science, Embase, OVID, Cochrane Libriary, CNKI, and Google Scholar databases were searched for relevant randomized controlled trials or non-randomized controlled trials from inception to October 15, 2022. Methodological quality of the trials was assessed, relevant data were extracted, and RevMan 5.3 and Stata 15.0 software were used to perform the meta-analysis of parameters related to the consequences.

Results:
A total of 22 articles were included, including 1923 patients. Meta-analysis results showed that the treatment of BMMNCs has a significantly lower incidence of THA (odds ratio [OR] = 0.33; 95% confidence interval [CI] = 0.27–0.41, P < .00001), radiographic progression rate (OR = 0.37; 95% CI = 0.21–0.63, P = .0003) and visual analog score at 24 months (mean difference [MD] = −11.84; 95% CI = −14.86 to −8.82, P < .00001), and has higher Harris hip score (MD = 6.90; 95% CI = 4.56–9.24, P < .00001), improvement of visual analog score at 24 months (MD = 6.87; 95% CI = 1.84–11.89, P = .007) and Merle D’Aubigne and Postel hip score (MD = 0.79; 95% CI = 0.14–1.44, P = .02). But there was no significant difference in the Western Ontario and McMaster University Osteoarthritis index (MD = −6.32; 95% CI = −16.76 to 4.12, P = .24) and incidence of complication (OR = 0.86; 95% CI = 0.52–1.42, P = .56).

Conclusion:
Current evidence supports that BMMNCs therapy could reduce the mid- and long-term rate of THA, improve hip function, alleviated the degree of hip pain, delay the progression of imaging staging and not increase the rate of complication, which maybe serve as a preferred option for treating ONFH.
A case report of Trousseau syndrome
Authors: Liu Xiao Jing , Liu Yu Xiang , Zhang Ning Yuan 
Published: October 2023

Rationale:
In 1865, Trousseau first discovered pulmonary embolism caused by multiple venous thrombosis in patients with gastric cancer, and later all clinical manifestations of malignant patients during pathogenesis due to abnormal coagulation and fibrinolysis were referred to collectively as Trousseau syndrome. Trousseau syndrome is not a benign thrombophlebitis, and when diagnosed it requires immediate treatment. The survival rate over 1 year is only 12%. Stroke in cancer patients has distinct characteristics different from conventional stroke and has higher mortality.

Patient concerns:
A 54-year-old female presented to the Department of Otolaryngology with recurrent right nasal bleeding for 4 days. After surgery, the patient experienced 7 different cerebral infarction courses. Finally died of brain herniation.

Diagnosis:
The specific abnormal laboratory index is the increase of D-dimer, suggesting the hypercoagulation state. The patient developed multiple cerebral infarction, myocardial injury, renal infarction, splenic infarction, and lower extremity arterial thrombosis, and finally was diagnosed Trousseau syndrome.

Interventions:
In the treatment, aspirin and atorvastatin were selected, but it did not work very well. D-dimer were high, we used low molecular weight heparin, and D-dimer decreased significantly.

Outcomes:
Finally the patient died of brain herniation.

Conclusion:
The raise of D-dimer and typical magnetic resonance imaging manifestation which provides a greater basis for diagnosis. The specific abnormal laboratory index is the increase of D-dimer, which provides direction for treatment and helps to evaluate treatment effect.
Vasculitis mimicking immunoglobulin-G4-related disease with involvement of the submandibular and lacrimal glands and periaortitis: A case report
Authors: Kuribayashi Mamika , Odaka Hidesato , Takahashi Susumu , Tokairin Takuo , Kurokawa Hirokazu 
Published: October 2023

Introduction:
Vasculitis does not usually induce glandular tissue lesions. Herein, we present a case of vasculitis with a distribution similar to that of immunoglobulin-G4-related disease (IgG4-RD), including that of the submandibular and lacrimal glands, and periaortitis.

Case report:
A 70-year-old man presented with shortness of breath. Laboratory findings revealed an IgG4 level of 191 mg/dL, negative antineutrophil cytoplasmic antibody test, and C-reactive protein level of 8.33 mg/dL. Magnetic resonance imaging of the head and computed tomography of the neck revealed bilaterally enlarged submandibular and lacrimal glands. Neck-to-pelvis computed tomography revealed bilateral infiltrative shadows in the lower lobes of both lungs, mass shadows in both lungs, and periaortitis of the abdominal aorta extending to the common iliac artery. Thus, the patient was diagnosed with IgG4-related respiratory disease and periaortitis/periarteritis. Prednisolone was administered at a dose of 35 mg (0.6 mg/kg daily). The dose was gradually tapered while observing the effects of the treatment. Imaging findings indicated an improvement and the C-reactive protein and IgG4 levels decreased, indicating a successful treatment course. However, after reexamination of the pathological findings, the diagnosis changed from IgG4-RD to vasculitis. One year after treatment initiation, the patient symptoms have stabilized.

Conclusion:
Vasculitis can present with lesions and pathological findings similar to those of IgG4-RD.
Effect of total quality management intervention on nurse commitment and nurse performance: A quasi-experimental study
Authors: Alzoubi Majdi M. , KS Hayati , AM Rosliza , Al-Zoubi Khaled M. , AL-Mugheed Khalid , Alsenany Samira A. , Oweidat Islam , Abdelaliem Sally M.F. 
Published: October 2023

Background:
The purpose of this study is to design, implement, and evaluate the impact of a total quality management intervention on job performance and commitment among Jordanian nurses working in government hospitals.

Methods:
A quasi-experimental multiple time series was conducted starting in September 2017 and ending in June 2018. 140 nurses were sampled using the proportionate stratified random sampling technique; 132 were completed the study 67 the intervention group, while 65 in the control group.

Results:
There were no significant differences in nurses’ job performance or commitment between the 2 groups (control and intervention). A repeated measure MANOVA test for both groups revealed that the interaction between group and time was statistically significant (F (4, 127) = 144.841; P = .001; Wilk’s Λ = 0.180; η2 = .820), indicating that groups had a significantly different pattern of job performance and commitment over time. A repeated test The MANCOVA test for both groups across time revealed significant differences in nurses’ job performance and nurses’ commitment at a less than 0.05 significance level (F (2127) = 320.724; P = .001; Wilk’s Λ = 0.165; η2 = 0.835), and the overall effect of time was significant for all dependent variables (F (4125) = 36.879; P = .001; Wilk’s Λ = 0.459; η2 = 0.541).

Conclusion:
The educational intervention was effective in improving nursing job performance among the study sample. The improved commitment of respondents in the intervention group was attributed to the improvement in job performance.
Whole-Body 18F-FDG PET/CT Patlak Parametric Imaging of Hepatic Alveolar Echinococcosis
Authors: Maurer Alexander , Kotasidis Fotis , Deibel Ansgar , Burger Irene A. , Huellner Martin W. 
Published: October 2023

Abstract
We present dynamic 18F-FDG PET/CT acquisition in a 52-year-old old woman with histologically proven hepatic alveolar echinococcosis (AE). Metabolic rate of FDG images generated with traditional and relative Patlak analysis show the AE manifestation in the liver significantly better the static SUV images. Dynamic PET may thus have the potential to increase sensitivity in the assessment of hepatic AE manifestations. Such parametric images may offer complementary qualitative information and quantification superior to SUV images.
Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis
Authors: Knights Alexander J. , Farrell Easton C. , Ellis Olivia M. , Song Michelle J. , Appleton C. Thomas , Maerz Tristan 
Published: October 2023

ABSTRACTObjectiveSynovium is home to immune and stromal cell types that orchestrate inflammation following a joint injury; in particular, macrophages are central protagonists in this process. We sought to define the cellular and temporal dynamics of the synovial immune niche in a mouse model of post-traumatic osteoarthritis (PTOA), and to identify stromal-immune crosstalk mechanisms that coordinate macrophage function and phenotype.DesignWe induced PTOA in mice using a non-invasive tibial compression model of anterior cruciate ligament rupture (ACLR). Single cell RNA-seq and flow cytometry were used to assess immune cell populations in healthy (Sham) and injured (7d and 28d post-ACLR) synovium. Characterization of synovial macrophage polarization states was performed, alongside computational modeling of macrophage differentiation, as well as implicated transcriptional regulators and stromal-immune communication axes.ResultsImmune cell types are broadly represented in healthy synovium, but experience drastic expansion and speciation in PTOA, most notably in the macrophage portion. We identified several polarization states of macrophages in synovium following joint injury, underpinned by distinct transcriptomic signatures, and regulated in part by stromal-derived macrophage colony-stimulating factor signaling. The transcription factors Pu.1, Cebpα, Cebpβ, and Jun were predicted to control differentiation of systemically derived monocytes into pro-inflammatory synovial macrophages.ConclusionsWe defined different synovial macrophage subpopulations present in healthy and injured mouse synovium. Nuanced characterization of the distinct functions, origins, and disease kinetics of macrophage subtypes in PTOA will be critical for targeting these highly versatile cells for therapeutic purposes.
Individuals with knee osteoarthritis show few limitations in reactive stepping responses after gait perturbations
Authors: Boekesteijn R.J. , Keijsers N.L.W. , Defoort K. , Geurts A.C.H. , Smulders K. 
Published: October 2023

AbstractBackgroundKnee osteoarthritis (OA) causes structural joint damage. The resultant symptoms can impair the ability to recover from unexpected gait perturbations, contributing to an increased fall risk. This study compared reactive stepping responses to gait perturbations between individuals with knee OA and healthy individuals.MethodsKinematic data of 35 individuals with end-stage knee OA, and 32 healthy individuals in the same age range were obtained during perturbed walking on a treadmill at 1.0 m/s. Participants received anteroposterior (trip or slip) or mediolateral perturbations during the stance phase. Changes from baseline in margin of stability (MoS), step length, step time, and step width during the first two steps after perturbation were compared between groups using a linear regression model. Extrapolated center of mass (XCoM) excursion was descriptively analyzed.FindingsAfter all perturbation modes, XCoM trajectories overlapped between individuals with knee OA and healthy individuals. Participants predominantly responded to mediolateral perturbations by adjusting their step width, and to anteroposterior perturbations by adjusting step length and step time. None of the perturbation modes yielded between-group differences in changes in MoS and step width during the first two steps after perturbation. Small between-group differences were observed for step length (i.e. 2 cm) of the second step after trip and slip perturbation, and for step time (i.e. 0.02 s) of the second step after slip perturbations.InterpretationDespite considerable pain and damage to the knee joint, individuals with knee OA showed comparable reactive stepping responses after gait perturbations to healthy participants.
Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalability
Authors: Cao Yunlong , Bai Yali , Yuan Tianjiao , Song Liyang , Fan Yu , Ren Liuhao , Song Weiliang , Peng Jiahui , An Ran , Gu Qingqing , Zheng Yinghui , Xie Xiaoliang Sunney 
Published: October 2023

AbstractExisting single cell bisulfite-based DNA methylation analysis is limited by low DNA recovery, and the measurement of 5hmC at single-base resolution remains challenging. Here we present a bisulfite-free single-cell whole-genome 5mC and 5hmC profiling technique, named Cabernet, which can characterize 5mC and 5hmC at single-base resolution with high genomic coverage. Cabernet utilizes Tn5 transposome for DNA fragmentation, which enables the discrimination between different alleles for measuring hemi-methylation status. Using Cabernet, we revealed the 5mC, hemi-5mC and 5hmC dynamics during early mouse embryo development, uncovering genomic regions exclusively governed by active or passive demethylation. We show that hemi-methylation status can be used to distinguish between pre– and post-replication cells, enabling more efficient cell grouping when integrated with 5mC profile. The property of Tn5 naturally enables Cabernet to achieve high-throughput single-cell methylome profiling, where we probed mouse cortical neurons and embryonic day 7.5 (E7.5) embryos, and constructed the library for thousands of single cells at high efficiency, demonstrating its potential for analyzing complex tissues at substantially low cost. Together, we present a new way of high-throughput methylome and hydroxymethylome detection at single-cell resolution, enabling efficient analysis of epigenetic status of biological systems with complicated nature such as neurons and cancer cells.Significance StatementMost of current methylation profiling techniques rely on bisulfite treatment, which suffers low DNA recovery. The technique proposed in this study, named Cabernet, can be used to measure 5mC and 5hmC at single-base resolution with high genomic coverage. By using Tn5 transposome, hemi-methylation status can be measured and high-throughput methylome profiling can be achieved. Together, it provides an efficient way to analyze the epigenetic landscape of complicated biological systems.
Statistical analysis plan for the “Does longer peripheral intravenous catheter Length optimise Antimicrobial Delivery: a randomised controlled trial to reduce interruptions to intravenous antimicrobial delivery (The LEADER Study)”
Authors: El-Heneidy Asmaa , Ware Robert S. , O’Brien Catherine , Larsen Emily , Marsh Nicole , Corley Amanda 
Published: October 2023

ABSTRACTThe “Does longer peripheral intravenous catheter (PIVC) Length optimise Antimicrobial Delivery: a randomised controlled trial to reduce interruptions to intravenous antimicrobial delivery (The LEADER Study)”, is a randomised controlled trial (RCT). This RCT aims to assess if long peripheral intravenous catheters (PIVCs) (4.5-6.4 cm) when compared with short-PIVCs (<4 cm) optimise antimicrobial delivery by reducing PIVC failure rates.The purpose for this document is to minimise bias and ensure transparency and internal validity for the findings of the trial, by defining and making publicly available the analysis approach prior to reviewing or analysing trial data. The statistical analysis plan (SAP) will inform analysis and reporting of the main effectiveness findings of the trial. It provides a detailed description of the primary and secondary trial outcomes and the methods for statistical comparison.
Fourth dose of Microneedle Array Patch of SARS-CoV-2 S1 Protein Subunit Vaccine Elicits Robust Long-lasting Humoral Responses in mice
Authors: Kim Eun , Shin Juyeop , Ferrari Alessandro , Huang Shaohua , An Eunjin , Han Donghoon , Khan Muhammad S. , Kenniston Thomas W. , Cassaniti Irene , Baldanti Fausto , Jeong Dohyeon , Gambotto Andrea 
Published: October 2023

AbstractThe COVID-19 pandemic has underscored the pressing need for safe and effective booster vaccines, particularly in considering the emergence of new SARS-CoV-2 variants and addressing vaccine distribution inequalities. Dissolving microneedle array patches (MAP) offer a promising delivery method, enhancing immunogenicity and improving accessibility through the skin’s immune potential. In this study, we evaluated a microneedle array patch-based S1 subunit protein COVID-19 vaccine candidate, which comprised a bivalent formulation targeting the Wuhan and Beta variant alongside a monovalent Delta variant spike proteins in a murine model. Notably, the second boost of homologous bivalent MAP-S1(WU+Beta) induced a 15.7-fold increase in IgG endpoint titer, while the third boost of heterologous MAP-S1RS09Delta yielded a more modest 1.6-fold increase. Importantly, this study demonstrated that the administration of four doses of the MAP vaccine induced robust and long-lasting immune responses, persisting for at least 80 weeks. These immune responses encompassed various IgG isotypes and remained statistically significant for one year. Furthermore, neutralizing antibodies against multiple SARS-CoV-2 variants were generated, with comparable responses observed against the Omicron variant. Overall, these findings emphasize the potential of MAP-based vaccines as a promising strategy to combat the evolving landscape of COVID-19 and to deliver a safe and effective booster vaccine worldwide.
Equivalent conversion investigation of environmental corrosion of suspenders in long-span suspension bridge
Authors: Yuan Zhijie , Wang Hao , Li Rou , Mao Jianxiao , Gao Hui 
Published: October 2023

Purpose
This paper aims to investigate the equivalent relationship between accelerated corrosion tests and real environmental spectrum of suspenders in long-span suspension bridge considering multiple factors action.

Design/methodology/approach
Based on Faraday's law, corrosion current was used as a measure of metal corrosion, and the equivalent conversion relationship between laboratory environment and real service environment was established. The equivalent conversion method for bridge structural steel had been determined under different temperature, humidity, pH value and NaCl concentration conditions. The compilation of environmental spectra for large span bridges considering multiple factors and the principle of equivalent conversion have been proposed.

Findings
Environmental factors, including temperature, humidity, pH value and NaCl concentration, have significant impact on the corrosion degree of suspension steel wires, and only considering these two factors for equivalent conversion cannot accurately reflect the true service environment of the bridge. The 33.8-h salt spray accelerated corrosion test using the standard conditions can be equivalent to one year of suspenders corrosion in a real service environment.

Originality/value
The equivalent accelerated corrosion method for steel wires proposed in this study can effectively predict the corrosion degree of the suspenders, which has been verified to be correct and can provide theoretical guidance for the development of corrosion test plans for steel wires and engineering technical basis for anti-corrosion control and calendar life research of suspension bridge suspenders.