bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution of genotypic and phenotypic diversity in multispecies biofilms
Authors: Amador Cristina I. , Moscovitz Sofia Zoe , Maccario Lorrie , Herschend Jakob , Kramer Isabel-Sophie , Jeckel Hannah , Cooper Vaughn S. , Drescher Knut , Neu Thomas R. , Burmølle Mette , Røder Henriette L. 
Published: October 2023

AbstractThe fitness and adaptability of bacteria in microbial communities can be influenced by the interactions among the community partners, as well as specific spatial organisation. Here, we investigated the evolutionary dynamics ofBacillus thuringiensisin response to the presence or absence of spatial structure and bacterial interspecies interactions. During evolution experiments, a distinct phenotypic variant ofB. thuringiensisfrequently occurred, irrespective of the conditions tested: planktonic vs. biofilm or monospecies vs. mixed species. Remarkably, selection significantly favored this variant ofB. thuringiensisover its ancestor in biofilm settings and when coexisting withPseudomonas defluviiandPseudomonas brenneri, co-isolated from a wastewater facility. Interestingly, the evolved phenotype displayed lower biomass in mixed species biofilms, shorter generation time, and lacked sporulation compared to the ancestor. The strains displaying this phenotypic variation contained mutations in the regulatorspo0Athat initiates sporulation but also de-represses expression of matrix determinants in other Bacilli. Consistently, matrix proteomics revealed a reduced abundance of TasA in the phenotypic variant, a major biofilm matrix component inBacillusspecies, while higher abundance was found in co-culture withP. brenneri. Our results indicate that interspecies interactions within biofilms not only promoteB. thuringiensisdiversification but also select for altered traits such as reduced biofilm matrix production. Moreover,P. brenneriwas favoured in co-culture biofilm vs. planktonic settings, suggesting that spatial structure andB. thuringiensisdiversification facilitate species co-existence. These findings could impact applications whereBacillusandPseudomonasare utilized in consortia, such as plant growth promoters or biopesticides.
Nitrosative stress under microaerobic conditions triggers inositol metabolism inPseudomonas extremaustralis
Authors: Venero Esmeralda C. Solar , Giambartolomei Lucia , Sosa Ezequiel , Fernández do Porto Darío , López Nancy I. , Tribelli Paula M. 
Published: October 2023

AbstractBacteria are exposed to reactive oxygen and nitrogen species that provoke oxidative and nitrosative stress which can lead to macromolecule damage. Coping with stress conditions involves the adjustment of cellular responses, which helps address metabolic challenges. In this study, we performed a global transcriptomic analysis of the response ofPseudomonas extremaustralisto nitrosative stress, induced by S-nitrosoglutathione (GSNO), a nitric oxide donor, under microaerobic conditions. The analysis revealed the upregulation of genes associated with inositol catabolism; a compound widely distributed in nature whose metabolism in bacteria has aroused interest. The RNA-seq data also showed heightened expression of genes involved in essential cellular processes like transcription, translation, amino acid transport and biosynthesis, as well as in stress resistance including iron-dependent superoxide dismutase, alkyl hydroperoxide reductase, thioredoxin, and glutathione S-transferase in response to GSNO. Furthermore, GSNO exposure differentially affected the expression of genes encoding nitrosylation target proteins, encompassing metalloproteins and proteins with free cysteine and /or tyrosine residues. Notably, genes associated with iron metabolism, such as pyoverdine synthesis and iron transporter genes, showed activation in the presence of GSNO, likely as response to enhanced protein turnover. Physiological assays demonstrated thatP. extremaustraliscan utilize inositol proficiently under both aerobic and microaerobic conditions, achieving growth comparable to glucose-supplemented cultures. Moreover, supplementing the culture medium with inositol enhances the stress tolerance ofP. extremaustralisagainst combined oxidative-nitrosative stress. Concordant with the heightened expression of pyoverdine genes under nitrosative stress, elevated pyoverdine production was observed whenmyo-inositol was added to the culture medium. These findings highlight the influence of nitrosative stress on proteins susceptible to nitrosylation and iron metabolism. Furthermore, the activation ofmyo-inositol catabolism emerges as a protective mechanism against nitrosative stress, shedding light on this pathway in bacterial systems, and holding significance in the adaptation to unfavorable conditions.
Types and outcomes of health promotion interventions led by public health students: a scoping review
Authors: Gonzalez-Aquines Alejandro , Bingham Daniel D , Kiilu Elizabeth M 
Published: October 2023

AbstractBackgroundPublic health students can contribute to improving population health outcomes; however, this remains an under-researched area. This review aims to assess the extent of the literature related to health promotion interventions led by public health students. This includes what these interventions are and how they are being conducted, as well as their impact on targeted populations.MethodsA scoping review was conducted according to JBI guidelines. A search strategy was developed using the population, context, concept framework. MEDLINE, CINAHL, Embase and Google Scholar were consulted. The selection process involved the screening of titles and abstracts against the inclusion criteria, followed by a full-text screening and data extraction by two reviewers. Disagreements were solved by consensus.Results191 studies were identified, but only five of these were included. The target populations included the general public, university students, and minority groups. Students were trained or supervised by experienced staff. The interventions were delivered using diverse means, including innovative approaches through social media. The student-led interventions increased access to preventive services (screening, vaccinations) and improved health knowledge within the target groups.ConclusionPublic health students make impactful contributions by increasing both the access to and the use of preventive services, as well as by promoting health knowledge. Our study contributes to the professionalisation of public health, highlighting for the first time the role of public health students in improving health outcomes. Furthermore, our study sheds light on the students’ impact on the reduction of health inequalities, particularly amongst minority groups.
Vinculin-Arp2/3 Interaction Inhibits Branched Actin Assembly to Control Cell Migration and Cell Cycle Progression
Authors: James John , Fokin Artem I. , Guschin Dmitry Y. , Wang Hong , Polesskaya Anna , Rubtsova Svetlana N. , Clainche Christophe Le , Silberzan Pascal , Gautreau Alexis M. , Romero Stéphane 
Published: October 2023

AbstractVinculin is a mechanotransducer that reinforces links between cell adhesions and linear arrays of actin filaments upon myosin-mediated contractility. Both adhesions to the substratum and neighboring cells, however, are initiated within membrane protrusions that originate from Arp2/3-nucleated branched actin networks. Vinculin has been reported to interact with the Arp2/3 complex, but the role of this interaction remains poorly understood. Here we compared the phenotypes of vinculin knock-out (KO) cells with those of knock-in (KI) cells, where the point mutation P878A that impairs the Arp2/3 interaction is introduced in the two vinculin alleles of MCF10A mammary epithelial cells. The interaction of vinculin with Arp2/3 inhibits actin polymerization at membrane protrusions and decreases migration persistence of single cells. In cell monolayers, vinculin recruits Arp2/3 and the vinculin-Arp2/3 interaction participates in cell-cell junction plasticity. Through this interaction, vinculin controls the decision to enter a new cell cycle as a function of cell density.
Three-way interaction and multiple mediation models: Environmental strategy and sustainability marketing commitment applications
Authors: Yu Tai-Yi , Horng Jeou-Shyan , Liu Chih-Hsing , Chou Sheng-Fang , Lee Ming-Tsung , Huang Yung-Chuan , Lapuz Maria Carmen B. 
Published: October 2023

PurposeThis study attempts to fill the research gap by extending sustainability literature and providing empirical evidence that considers sustainability marketing commitment (SMC) as a fundamental attribute of effective marketing strategy that consequently improves tourism service quality, as represented by service attractiveness.Design/methodology/approachIn the current study, data was collected from 313 tourism and hospitality firms. To test the model, this study applied structural equation modeling (SEM) to investigate the relationships among environmental strategy, SMC, supplier trust and service attractiveness in a mediation-moderation setting.FindingsThe results indicate that the multiple mediation effects of environmental strategy may indirectly influence tourist attractiveness through SMC and tourism services. The two-way moderating effects reveal that supplier trust and socialization strengthen the service attractiveness development process, while three-way interaction discovered that socialization and supplier trust positively moderate the relationships between tourism services and service attractiveness.Originality/valueSustainable strategy is a future trend for tourism business management; however, unknown to most is the role of marketing and environmental strategy in tourism business due to lack of integration with concepts in marketing strategy, with the multidimensionality of tourism services, and with the function of trust and socialization, critically undermining analyses of service attractiveness. This paper combines corporate sustainability and sustainability marketing methods to explore how an environmental strategy can improve tourism services and enhance a destination's attractiveness based on a mediation-moderation mechanism.
Fostering comparative advantage: the roles of data-driven competitive sustainability, green product innovation and green process innovation through moderated-mediation model
Authors: AL-Shboul Moh'd Anwer 
Published: October 2023

PurposeThis study attempts to examine the relationship between reliable big and cloud data analytics capabilities (RB&CDACs) and comparative advantages (CA) of manufacturing firms (MFs) in the Middle East region as developing countries using green product innovation (GPI) and green process innovations (GPrI) mediating factors, further assess the role of data-driven competitive sustainability factor as a moderated factor.Design/methodology/approach436 useable online surveys were analyzed using the quantitative approach for the data-gathering process, applying structural equation modeling in the Smart-PLS program as an analysis tool. The sample unit for analysis included all middle- and senior-level managers and employees within MFs. The authors performed convergent validity and discriminant validity tests, bootstrapping also was applied. The authors included GPI and GPrI as mediating factors while using data-driven competitive sustainability as a moderated factor.FindingsThe findings of this study indicated that there is a positive significant effect in the relationship between reliable big and cloud data analytics capabilities and comparative advantages, which is supported by the formulated hypothesis. Furthermore, the findings confirmed that there was a positive and significant effect through the mediating factors (i.e. GPI and GPrI) on comparative advantage, additionally, it confirmed and supported that the moderating factor represented by data-driven competitive advantage suitability has significant effect as well.Research limitations/implicationsThis study has some limitations represented by using only one type of methodological approach (i.e. quantitative), further, it was conducted on only Asian countries in the Middle East region.Originality/valueThis piece of work improved the proposed conceptual research model and included several factors such as reliable big and cloud data analytics capabilities, comparative advantage, data-driven competitive sustainability, GPI and GPrI. This research offered new and valuable information and findings for managers, practitioners and decision-makers in the MFs in the Middle East region as a road map and gaudiness for the importance to apply these factors in their firms for enhancing the comparative advantages in their firms. Further, this research fills the gap in SCM literature and makes a bridge of knowledge and contribution to the existence of previous studies.
How does top management team regulatory focus influence management innovation and performance in megaprojects: the moderating role of project uncertainties
Authors: Ma Li , Lu Yongqiang 
Published: October 2023

PurposeExisting research on innovation has mainly focused on how to promote technological innovation in megaprojects and management innovation (MI) in megaprojects is still an unknown research field. The purposes of this study are to examine the effect of MI on megaproject performance and how the top management team (TMT) regulatory focus affects the use of MI in projects. At the same time, the moderating effects of project uncertainties are also tested.Design/methodology/approachOn the basis of an explorative/exploitative ambidextrous analysis framework, this study divides MI into two dimensions: explorative and exploitative MI, and integrates the theoretical perspectives of the TMT regulatory focus and project uncertainties into a research model. Taking 314 responses from megaprojects’ TMTs in China as research data, this study empirically tests the above model.FindingsResults show that exploratory MI has a U-shaped relationship with megaproject performance; whereas exploitative MI has an inverted U-shaped relationship with megaproject performance. The TMT promotion focus has a positive effect on exploratory and exploitative MI; and the TMT prevention focus has a negative effect on exploratory MI but has a positive effect on exploitative MI. Project uncertainties have a positive moderating effect on the positive relationship between TMT promotion focus and exploratory MI, whereas it has a negative moderating effect on the negative relationship between the TMT prevention focus and exploratory MI.Originality/valueBy empirically measuring the relationship between two types of MIs and megaproject performance, this study clarifies the differential mechanism of the effect of different MIs on megaproject performance. This study also examines the MI of megaprojects from the perspective of the TMT regulatory focus and expounds how changes in uncertainties affect the relationship between the TMT regulatory focus and MI.
Has the pandemic affected the motivation and job satisfaction of university researchers? A case study from France
Authors: Goncharuk Anatoliy , Vinot Didier 
Published: October 2023

PurposeThe purpose of this study is to determine whether the pandemic affects the motivation and job satisfaction of university researchers as a whole and by their separate groups by gender, age, academic position (career stage) and degree.Design/methodology/approachThe authors studied French universities for changes in motivation and job satisfaction among researchers under the impact of the COVID-19 pandemic. For this, two hypotheses were tested, using the one-way ANOVA parametric test and the Kruskal–Wallis nonparametric statistical test with the data collected during the in-depth interviews and surveys with university researchers from the Paris Diderot University and Jean Moulin University of Lyon III in 2019 and 2021.FindingsThere were found significant differences between changes in motivation and job satisfaction of respondent groups by gender, career stage and scientific degrees. All these differences in changes confirm hypothesis about the significant impact of the pandemic on certain motivators and elements of job satisfaction of various groups of university researchers. The study showed that pandemic restrictions and fears positively affect the motivation and job satisfaction of the younger generation of researchers and males, and negatively on the older generation and female researchers. During the pandemic, early-stage researchers are mostly unhappy only with the salary and the lack of teaching skills. While experienced and senior researchers do not have time to fulfill all their duties due to overload by a combination of different forms of teaching, many teaching hours, administrative duties and mastering new communication tools.Research limitations/implicationsThe results capture the selective impact of pandemic restrictions, its physical and mental effects on academic staff. Although only two universities in a single country were considered, the findings show the heterogeneity of the perception of new (pandemic) working conditions by different groups of university workers. If such heterogeneity is revealed in the example of two universities, then it is very likely to be observable also in broader studies.Practical implicationsThe results of this study can help university administrations, higher education regulators and national research agencies address the needs of all groups of researchers, alleviating the most acute restrictions and fears caused by the pandemic and providing the necessary incentives for relevant groups, e.g. higher salary, teaching school and awards for young researchers and a lower teaching loading for experienced and senior researchers.Originality/valueThis is the first study that define pandemic impact on motivation and job satisfaction of university researchers in France.
Effects of time-varying political connections on loan contracts
Authors: Fang Hao , Wang Chieh-Hsuan , Shieh Joseph C.P. , Chung Chien-Ping 
Published: October 2023

PurposeThe authors construct two time-varying political connection (PC) indexes to measure a firm's political tendencies toward ruling and opposing parties and analyze whether a firm with ruling party tendencies obtains better bank loan contracts compared to the contracts obtained by a firm with opposing party tendencies and a firm with fixed PC tendencies.Design/methodology/approachLinguistic text mining is used to construct the two time-varying PC indexes from news sources that reflect the tone and frequencies of characteristic texts to determine a firm's tendencies to favor the ruling or opposing parties.FindingsThe results show that varying PC firms connected to the ruling party receive preferential loan contracts when their political tendencies increase but varying PC firms connected to the opposition party do not. In contrast, fixed PC firms gain similar benefits only when the connection is determined in the presidential election year but not in other years. Firms supporting two parties receive minimal financial rewards in terms of loan terms.Originality/valueIn past studies, once a firm is identified as having a connection with a political party, it is assumed to have PC throughout the sample period (i.e. fixed PC firms). The authors lift this assumption and examine how varying PC affect bank loan contracts. The two time-varying PC indexes can identify a firm's more immediate party tendencies and more precise effects of a firm's party tendencies on bank loan contracts.
Reflections on gaps in public sector leadership development theory
Authors: Marcy Richard T. 
Published: October 2023

PurposeThis paper explores some of the gaps and contradictions that can often be found in public sector leader development approaches and then examines some of the theoretical assumptions related to these gaps and contradictions. The purpose of this examination is to further identify and define some corresponding gaps within public sector leadership development theory, as well as to encourage future theory development.Design/methodology/approachUsing three common examples of contradictions often found within the leadership literature, gaps within public sector leadership development theory are identified and then linked to relevant components of leader development models found within other disciplines. As they were developed in other contexts, these components were then further reviewed to determine their potential applicability in speaking to the gaps often found in public sector leader development theory.FindingsProposals are made to address some of the common gaps and contradictions often identified in some public sector leader development approaches, along with the detailing of future research directions for the further development of theory.Originality/valueThis exploratory review highlights some of the central assumptions and gaps in the literature for the purpose of clarifying future directions of research into public sector leadership development theory.
Investigating potential tourists' attitudes toward artificial intelligence services: a market segmentation approach
Authors: Choe Ja Young (Jacey) , Opoku Emmanuel Kwame , Cuervo Javier Calero , Adongo Raymond 
Published: October 2023

PurposeThis study profiles and segments potential tourists on the basis of their various attitudes toward artificial intelligence (AI) services. Furthermore, this study distinguishes descriptors among the different clusters, such as preference for using diverse AI services, overall image of AI services, willingness to use AI services (WUAI), willingness to pay more for AI services (WPAI) in tourism and hospitality, and characteristics of respondents.Design/methodology/approachAn online survey was conducted in South Korea. Data on 758 potential tourists were used for K-means cluster analysis.FindingsThis study identified three distinct tourist segments with differentiated attitudes toward AI services: the group aspiring to use or fantasizing about AI services (Cluster 1), the group being knowledgeable and supportive of AI services (Cluster 2), and the group having low interest about AI services (Cluster 3).Practical implicationsMembers of Cluster 2 were the most marketable as this segment exhibited the greatest knowledge of and support for AI services, while Cluster 1 would be an ideal segment to launch and test novel AI services.Originality/valueThis study extends the authors’ knowledge of AI scholarship by unpacking the existing market segments, which could be tapped to sustain AI penetration in the tourism industry. Hence, this study contributes to existing debates on AI scholarship, which is predominated by conceptual reflections and issues of AI services in the tourism and hospitality field.