bluuebunny/crossref_metadata_2025_split huggingface dataset:
SAT128 Low Concentration Of Butyrate Reverts PA-induced Atrophic Condition On C2C12 Myotubes
Authors: Tini Sabrina , Antonioli Alessandro , Reano Simone , Raiteri Tommaso , Scircoli Andrea , Antoniotti Valentina , Zaggia Ivan , Filigheddu Nicoletta , Prodam Flavia 
Published: October 2023

Abstract
Disclosure: S. Tini: None. A. Antonioli: None. S. Reano: None. T. Raiteri: None. A. Scircoli: None. V. Antoniotti: None. I. Zaggia: None. N. Filigheddu: None. F. Prodam: None.
Sarcobesity is a well-defined medical condition characterized by the coexistence between skeletal muscle atrophy and increased fat mass representing, nowadays, a relevant public health issue among the world population. Sarcobesity is often associated with the development of metabolic syndrome (MetS), insulin-resistance (IR), dyslipidaemia, and other pathologies such as cardiovascular diseases (CVDs) and type 2 diabetes (T2DM). The global increment of this condition has been favoured by changes in lifestyle and dietary habits as largely demonstrated. Particularly, Western Diet (WD), rich in saturated fats and sugars and poor in fibers, vitamins, and minerals seems to be the main cause of sarcobesity in association with low levels of physical activity. In recent years, Ketogenic Diet (KD) has been proposed as a fighting obesity therapy and as a new possible therapeutic strategy to counteract the WD detrimental effects on different tissues, i.e skeletal muscle that plays a key role in whole-body metabolism, glucose homeostasis and lipid use. KD regimen consists in a very low carbohydrates intake, high fat and adequate protein contents that cause the so-called “nutritional ketosis” with the production of Ketone bodies (KBs). KBs, such as beta-hydroxybutyrate (BHB), acetoacetate and acetone are the final products of the partial beta-oxidation of free fatty acid. They are short chain fatty acid produced in liver with different role on metabolism and they are used as a source of energy instead of glucose by different tissues including skeletal muscle. Preliminary experiments in our laboratory demonstrated that palmitate (PA), which is a saturated fat highly present in WD regimen, induces myotubes atrophy in dose dependent manner (100, 250, 500uM). The aim of the present study is to investigate not only if KBs have a direct effect on C2C12 skeletal muscle cells, but also if they are able to contrast the PA-induced atrophy in myotubes. We demonstrated with a co-treatment that sodium butyrate (s-BU), which is the main KB produced during ketogenesis, is able to prevent PA negative effects in skeletal muscle cells when it is used at low concentration. In contrast, high doses of BU reduced C2C12 diameters suggesting that KBs could have opposite effects depending on their concentration. Currently, other short chain fatty acids and KBs, i.e pyruvate and acetoacetate are under investigation in our laboratory. Collectively, these data suggest that KD could represent a promising intervention to contrast WD damages on skeletal muscle in overweight/obese individuals.
Presentation: Saturday, June 17, 2023
‘Chip’-ing away at morphogenesis – application of organ-on-chip technologies to study tissue morphogenesis
Authors: White Matthew J. , Singh Tania , Wang Eric , Smith Quinton , Kutys Matthew L. 
Published: October 2023

ABSTRACT
Emergent cell behaviors that drive tissue morphogenesis are the integrated product of instructions from gene regulatory networks, mechanics and signals from the local tissue microenvironment. How these discrete inputs intersect to coordinate diverse morphogenic events is a critical area of interest. Organ-on-chip technology has revolutionized the ability to construct and manipulate miniaturized human tissues with organotypic three-dimensional architectures in vitro. Applications of organ-on-chip platforms have increasingly transitioned from proof-of-concept tissue engineering to discovery biology, furthering our understanding of molecular and mechanical mechanisms that operate across biological scales to orchestrate tissue morphogenesis. Here, we provide the biological framework to harness organ-on-chip systems to study tissue morphogenesis, and we highlight recent examples where organ-on-chips and associated microphysiological systems have enabled new mechanistic insight in diverse morphogenic settings. We further highlight the use of organ-on-chip platforms as emerging test beds for cell and developmental biology.
Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure
Authors: Barger Sarah R. , Penfield Lauren , Bahmanyar Shirin 
Published: October 2023

ABSTRACT
Barrier-to-autointegration factor (BAF) protein is a DNA-binding protein that crosslinks chromatin to allow mitotic nuclear envelope (NE) assembly. The LAP2-emerin-MAN1 (LEM)-domain protein LEMD2 and ESCRT-II/III hybrid protein CHMP7 close NE holes surrounding spindle microtubules (MTs). BAF binds LEM-domain family proteins to repair NE ruptures in interphase, but whether BAF–LEM binding participates in NE hole closure around spindle MTs is not known. Here, we took advantage of the stereotypical event of NE formation in fertilized Caenorhabditis elegans oocytes to show that BAF–LEM binding and LEM-2–CHMP-7 have distinct roles in NE closure around spindle MTs. LEM-2 and EMR-1 (homologs of LEMD2 and emerin) function redundantly with BAF-1 (the C. elegans BAF protein) in NE closure. Compromising BAF–LEM binding revealed an additional role for EMR-1 in the maintenance of the NE permeability barrier. In the absence of BAF–LEM binding, LEM-2–CHMP-7 was required for NE assembly and embryo survival. The winged helix domain of LEM-2 recruits CHMP-7 to the NE in C. elegans and a LEM-2-independent nucleoplasmic pool of CHMP-7 also contributes to NE stability. Thus, NE hole closure surrounding spindle MTs requires redundant mechanisms that safeguard against failure in NE assembly to support embryogenesis.
Tiered Assortment: Optimization and Online Learning
Authors: Cao Junyu , Sun Wei 
Published: October 2023

Due to the sheer number of available choices, online retailers frequently use tiered assortment to present products. In this case, groups of products are arranged across multiple pages or stages, and a customer clicks on “next” or “load more” to access them sequentially. Despite the prevalence of such assortments in practice, this topic has not received much attention in the existing literature. In this work, we focus on a sequential choice model that captures customers’ behavior when product recommendations are presented in tiers. We analyze multiple variants of tiered assortment optimization (TAO) problems by imposing “no-duplication” and capacity constraints. For the offline setting involving known customers’ preferences, we characterize the properties of the optimal tiered assortment and propose algorithms that improve the computational efficiency over the existing benchmarks. To the best of our knowledge, we are the first to study the online setting of the TAO problem. A unique characteristic of our online setting, absent from the one-tier MNL bandit, is partial feedback. Products in the lower priority tiers are not shown when a customer has purchased a product or has chosen to exit at an earlier tier. Such partial feedback, along with product interdependence across tiers, increases the learning complexity. For both the noncontextual and contextual problems, we propose online algorithms and quantify their respective regrets. Moreover, we are able to construct tighter uncertainty sets for model parameters in the contextual case and thus improve the performance. We demonstrate the efficacy of our proposed algorithms through numerical experiments. This paper was accepted by J. George Shanthikumar, data science. Supplemental Material: The data files and e-companion are available at https://doi.org/10.1287/mnsc.2023.4940 .
Assessing Environmental Mitigation Strategies in Controlled Cage-Free Aviary Housing Systems: Effect on Air Quality and Bioaerosols
Authors: St-Germain Magali-Wen , Létourneau Valérie , Larios Araceli Dalila , Godbout Stephane , Fournel Sébastien , Duchaine Caroline 
Published: October 2023

Highlights

Three mitigation strategies were tested in experimental chambers fitted as small-scale aviaries.

Particle monitor and high-flow air samplers were used to sample airborne dust (PM) and bioaerosols.

Oil emulsion sprinkling on the litter surface of aviaries was effective in reducing PM emissions in experimental chambers.

Abstract. Alternative housing systems for laying hens are gradually replacing battery cages. Though these systems provide more space per animal and enrichments, higher concentrations of ammonia and airborne dust have been reported in those environments. In this project, three environmental control strategies and a control condition were tested in twelve experimental chambers fitted as small-scale aviaries. The experiment was conducted during three periods spanning 18 weeks, during which PM and bioaerosols were sampled in each chamber in three separate sampling campaigns. Emissions of PM, culturable bacteria and molds, total bacteria, Penicillium, and Aspergillus molds and selected fecal indicators (Campylobacter coli, Campylobacter jejuni, Clostridium perfringens, Escherichia coli, Enterococcus spp., Salmonella spp.) were assessed in each experimental chamber. Strategies that included oil emulsion sprinkling on litter surface were effective at reducing PM emissions, while no significant effect (p &gt; 0.05) was observed on ammonia emissions, and the effect on bioaerosol was variable under the evaluated conditions. Keywords: Airborne dust, Aviaries, Bioaerosol, Laying hen alternative housing, Mitigation.
Streambank Erosion Phenomena and Understanding: Current Research and Future Directions
Authors: Bolinaga Celso Castro , Mittelstet Aaron , Mankin Kyle 
Published: October 2023

Highlights

High rates of streambank erosion are detrimental to the stability and function of streams.

This collection brings together six studies that represent key advances in streambank erosion research.

Current research directions on streambank erosion, erodibility characterization, and sediment loading are presented.

Future research directions and challenges related to high-frequency data collection and modeling are discussed.

Abstract. Streams are in dynamic equilibrium with their environments, and as that environment is altered by human development and changing climate, streambank erosion is a common, but little understood, result. This article highlights the contributions of the special collection “Streambank Erosion, Sediment Dynamics, and Restoration (SER),” which assembled six studies that represent key advances in streambank erosion research, highlight current research in the field, and identify directions for future research. The studies in this special collection were grouped into three central themes: (1) streambank erosion monitoring, (2) streambank erodibility characterization, and (3) streambank erosion loading. In this article, key findings within each of these central themes are summarized, emphasizing the significant contributions of each study. Likewise, perspectives on future research directions are discussed, outlining important challenges that remain to be addressed. Overall, the studies in this special collection are unified in their overarching goal of improving quantitative and predictive understanding of streambank erosion phenomena. Keywords: Keywords., Erodibility Parameters, Jet Erosion Test, Monitoring, Sediment, Soil Erosion, Stabilization Practices, Streambank, Stream Restoration.
Exploring the potential of broadband Tamm plasmon resonance for enhanced photodetection
Authors: Poddar Krishanu , Sinha Rajarshi , Jana Babita , Chatterjee Supratim , Mukherjee Rupam , Maity Amit Ranjan , Kumar Samir , Maji Partha Sona 
Published: October 2023

Tamm plasmon polaritons (TPPs) have emerged as a promising platform for photodetector applications due to their strong light–matter interaction and potential for efficient light absorption. In this work, a design for a broadband photodetector (PD) based on the optical Tamm plasmon (OTS) state generated in a periodic metal–semiconductor–distributed Bragg reflector (DBR) geometry is proposed. The transfer matrix method (TMM) was used to study the propagation of electromagnetic waves through the proposed structure. By exciting the structure with incident light and analyzing the electric field profile within the multilayer structure at the resonant wavelength, we observe a distinctive electric field distribution that indicates the presence of Tamm plasmon modes. A comparative study was conducted to investigate the optical properties of a photodetector in the near-infrared (NIR) range by varying parameters such as thickness. By optimizing the thickness, we successfully achieved a broadband photoresponse in the photodetector, with a maximum responsivity of 21.8 mA/W at a wavelength of 1354 nm, which falls within the photonic bandgap region. FWHM was found to be 590 nm for the responsivity spectrum. The geometry also presents maximum absorption with FWHM calculated to be about 871.5 nm. The proposed geometry offers a broadband photoresponse, which is advantageous for the advancement of Tamm-based detector technologies. The ability to detect light over a wide operation range makes this mechanism highly beneficial for various applications.
Position: A study protocol for the prevention of fall injuries in french special forces selection courses using a body-centered intervention
Authors: Obligi Loucas , Bertrand Mathieu , Boivent Mathieu , Corcostegui Simon-Pierre , Coz Pierre-Emmanuel , Derkenne Clément , Des Robert Vincent , Hurpin Victor , Hus Jauffrey , L’Hermitte Benoît , Lely Laurent , Patey Edouard , Romary Emeric , Saint-Jean Luc , Trente Alexandre , Turpin Marine , Vertu Nicolas , Verdonk Charles , Duffaud Anaïs M. 
Published: October 2023

Introduction
The Military Physical and Sports Training program was developed by the French Army in order to train, optimize, and maintain individual readiness. Although the health benefits of sport practice do not need to be demonstrated, such activities can cause acute musculoskeletal injuries that need to be addressed. The prevalence of lower limb injury is rather high in the French military population and, in particular, ranges from 15 to 45% during Special Forces selection courses. Thus, this project aims to investigate the efficiency of a body-centered program designed to enhance body awareness. The program seeks to train the mind to actively pay attention to body information, while the latter is viewed as a protective factor against fall injuries. We assume: (i) that postural control can be improved by enhancing the level of body awareness; and (ii) that greater postural awareness could be beneficial in reducing the risk of fall injuries. The body-centered prevention program is based on the Optimization of the Resources of the Armed Forces (ORAF) intervention, which focuses on mental preparation and recovery, and has been deployed in the French Army for many years.

Method and analyses
The study focuses on five French Special Forces selection courses (400 soldiers/ participants). It is divided into two stages (year 1, year 2). The first year is dedicated to data collection from the control group (200 participants), while in the second year the ORAF intervention will be deployed. In both year, participants will be subjected to the same enrollment schedule (Fig 3). The main objective is to evaluate the effectiveness of the ORAF intervention in reducing the rate of fall injuries during military selection, based on a multidisciplinary method that captures demographic, biological, biometric, clinical, and para-clinical measures.

Trial registration
Registration number: IDRCB number 2021-A02108-33, Clinical Trial: NCT 05451394.
Dietary fiber intake and cognitive impairment in older patients with chronic kidney disease in the United States: A cross-sectional study
Authors: Li Feiyan , Chen Hongxi , Mao Nan , Liu Hong 
Published: October 2023

Background
High-fiber diet has been associated with better cognitive performance. However, the association between dietary fiber intake and cognition in older patients with chronic kidney disease (CKD) remains unknown. Hence, this study aimed to investigate the effect of dietary fiber intake on cognition in older patients with CKD.

Methods
This study included participants aged ≥60 years who provided data on social demography, cognitive tests (Consortium to Establish a Registry for Alzheimer’s disease Word Learning [CERAD-WL], CERAD Delayed Recall [CERAD-DR], Animal Fluency Test [AFT], and Digit Symbol Substitution Test [DSST]), diet, and other potential cognition-related variables from the National Health and Nutrition Examination Survey 2011–2014. Fully-adjusted multivariate logistic regression subgroup models were performed, and multiple linear regression analyses were employed to examine the association between dietary fiber intake and cognition in patients with CKD.

Results
A total of 2461 older adults were included, with 32% who suffered from CKD. Participants with CKD scored lower in CERAD-WL, CERAD-DR, AFT, and DSST. Patients with CKD consuming low dietary fiber (≤25 g/day) had a higher risk of CERAD-WL and DSST impairments. High dietary fiber intake eliminated the differences in CERAD-WL and DSST impairments between the CKD and non-CKD participants. However, no associations were observed between CKD and CERAD-DR and AFT impairments regardless of dietary fiber intake. A positive linear relationship between dietary fiber intake and AFT score was observed in older patients with CKD.

Conclusion
High dietary fiber intake may benefit cognitive function in older patients with CKD. High-fiber diet management strategies could potentially mitigate cognitive impairment in this group of patients.
Investigating infectious outcomes in adult patients undergoing solid organ transplantation: A retrospective single-center experience, Paris, France
Authors: Tarhini Hassan , Waked Rami , Rahi Mayda , Haddad Nihel , Dorent Richard , Randoux Christine , Bunel Vincent , Lariven Sylvie , Deconinck Laurene , Rioux Christophe , Yazdanpanah Yazdan , Joly Veronique , Ghosn Jade 
Published: October 2023

Objectives
This study described the demographic characteristics, clinical presentation, treatment, and outcomes of solid organ transplant recipients who were admitted to our center for infection. It also determined factors associated with a poor outcome, and compares early and late period infections.

Methods
In this retrospective observational study, conducted at a tertiary care center in France between October 2017 and March 2019, infectious outcomes of patients with solid organ transplant where studied.

Results
A total of 104 patients were included with 158 hospitalizations for infection. Among these 104 patients, 71 (68%) were men. The median age was 59 years old. The most common symptoms on admission were fever (66%) and chills (31%). Lower respiratory tract infections were the most common diagnosis (71/158 hospitalizations). Urinary tract infections were frequently seen in kidney transplant recipients (25/60 hospitalizations). One or more infectious agents were isolated for 113 hospitalizations (72%): 70 bacteria, 36 viruses and 10 fungi, with predominance of gram-negative bacilli (53 cases) of which 13 were multidrug-resistant. The most frequently used antibiotics were third generation cephalosporins (40 cases), followed by piperacillin-tazobactam (26 cases). We note that 25 infections (16%) occurred during the first 6 months (early post-transplant period). Patients admitted during the early post-transplant period were more often on immunosuppressive treatment with prednisone (25/25 VS 106/133) (p = 0.01), mycophenolic acid (22/25 VS 86/133) (p = 0.03), presented for an urinary tract infection (10/25 VS 25/133) (p = 0.04) or a bacterial infection (17/25 VS 53/133) (p = 0.01). Patients with later infection had more comorbidities (57/83 VS 9/21) (p = 0.03), cancer (19/83 VS 0/21) (p = 0.04) or were on treatment with everolimus (46/133 VS 0/25) (p = 0.001). During 31 hospitalizations (20%), patients presented with a serious infection requiring intensive care (n = 26; 16%) or leading to death (n = 7; 4%). Bacteremia, pulmonary and cardiac complications were the main risk factors associated with poor outcome.

Conclusion
Infections pose a significant challenge in the care of solid organ transplant patients, particularly those with comorbidities and intensive immunosuppression. This underscores the crucial importance of continuous surveillance and epidemiologic monitoring within this patient population.
Multigene panel next generation sequencing in metastatic colorectal cancer in an Australian population
Authors: Nindra Udit , Pal Abhijit , Lea Vivienne , Lim Stephanie Hui-Su , Wilkinson Kate , Asghari Ray , Roberts Tara L. , Becker Therese M. , Farzin Mahtab , Rutland Tristan , Lee Mark , MacKenzie Scott , Ng Weng , Wang Bin , Lee C. Soon , Chua Wei 
Published: October 2023

Background
Next generation sequencing (NGS) is increasingly used in standard clinical practice to identify patients with potentially actionable mutations. Stratification of NGS mutation tiers is currently based on the European Society of Medical Oncology (ESMO) Scale for Clinical Actionability of Molecular Targets (ESCAT[E]) Tier I–V & X. Allele frequency is also increasingly recognised as an important prognostic tool in advanced cancer. The aim of this study was to determine the genomic mutations in metastatic colorectal cancer (CRC) in an Australian multicultural population and their influence on survival outcomes.

Methods
Next generation sequencing with the 50-gene panel Oncomine Precision Assay™ was used on 180 CRC tissue samples obtained across six Sydney hospitals between June 2021 and March 2022.

Results
From 180 samples, 147 (82%) had at least one gene mutation identified with 68 (38%) having two or more concurrent mutations. Tier I variants included RAS wild-type [EI] in 73 (41%) and BRAF V600E [EIA] in 27 (15%). Non-tier I variants include 2 (1%) ERBB2 amplification [EIIB], 26 (15%) PIK3CA hotspot mutations [EIIIA] and 9 (5%) MET focal amplifications [EIIIA]. NGS testing revealed an additional 22% of cases with Tier II & III mutations. 43% of patients also presented with potentially actionable Tier III & IV mutations. Patients with concurrent TP53 and RAS mutations had significantly reduced overall survival (6.1 months versus 21.1 months, p <0.01). High KRAS allele frequency, as defined by those with over 20% variant allele frequency (VAF), also demonstrated reduced overall survival (12.1 months versus 42.9 months, p = 0.04).

Conclusions
In addition to identifying patients with genomic alterations suitable for clinically proven standard of care therapeutic options, the 50 gene NGS panel has significant potential in identifying potentially actionable non-tier 1 mutations and therefore may become future standard clinical practice.