bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electronic Polarizability Tunes the Function of the Human Bestrophin 1 Cl⁻ Channel
Authors: Phan Linda X. , Owji Aaron P. , Yang Tingting , Crain Jason , Sansom Mark S.P. , Tucker Stephen J. 
Published: November 2023

AbstractMechanisms of anion permeation within ion channels and nanopores remain poorly understood. Recent cryo-electron microscopy structures of the human bestrophin 1 Cl⁻ channel (hBest1) provide an opportunity to evaluate ion interactions predicted by molecular dynamics (MD) simulations against experimental observations. Here, we implement the fully polarizable forcefield AMOEBA in MD simulations on different conformations of hBest1. This forcefield models multipole moments up to the quadrupole; therefore, it captures induced dipole and anion-π interactions. We show that key biophysical properties of the channel can only be simulated when electronic polarization is included in the molecular models and that Cl⁻ permeation through the neck of the pore is achieved through hydrophobic solvation concomitant with partial ion dehydration. Furthermore, we demonstrate how such polarizable simulations can help determine the identity of ion-like densities within high-resolution cryo-EM structures and that neglecting polarization places Cl⁻ at positions that do not correspond with their experimentally resolved location. Overall, our results demonstrate the importance of including electronic polarization in realistic and physically accurate models of biological systems, especially channels and pores that selectively permeate anions.Statement of SignificanceIon channels are nanoscale protein pores that enable the selective passage of charged ions across cell membranes. Understanding the underlying mechanisms for selective anion permeation through such pores remains a challenge. To simulate their behavior efficientlyin silico, fixed charge models are typically employed. However, this approach is insufficient for the study of anions. Here, we use simulations with explicit treatment of electrostatics to investigate the interactions of chloride ions in the human bestrophin 1 channel. We find that electronic polarization tunes the state of the channel and affects the interactions of chloride ions thereby revealing a mechanism for permeation. Furthermore, these simulations can be used to resolve experimental ambiguity in ion-like densities from cryo-EM structures.
The cell cycle controls spindle architecture in Arabidopsis by modulating the augmin pathway
Authors: Motta Mariana Romeiro , Nédélec François , Woelken Elke , Saville Helen , Jacquerie Claire , Pastuglia Martine , Stolze Sara Christina , Van De Slijke Eveline , Chen Poyu , Böttger Lev , Belcram Katia , Nakagami Hirofumi , De Jaeger Geert , Bouchez David , Schnittger Arp 
Published: November 2023

SummaryTo ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on specific microtubule structures called mitotic spindles. There are, however, striking differences in overall spindle organization among eukaryotic super groups, and in particular little is known about how spindle architecture is determined in plants. As a foundation for our work, we have measured prime characteristics of Arabidopsis mitotic spindles and built a three-dimensional dynamic model of the Arabidopsis mitotic spindle using Cytosim. Next, we identified the cell-cycle regulator CYCLIN-DEPENDENT KINASE B1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle shape and organization in Arabidopsis. Loss of CDKB1 function resulted in a high number of astral microtubules that are normally absent from plant spindles, as opposed to animal ones. We identified an augmin complex member, ENDOSPERM DEFECTIVE1 (EDE1), as a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant of EDE1 displayed spindles with extended pole-to-pole distance, resembling the phenotypes ofcycb3;1andcdkb1mutants. Moreover, we found that the mutated EDE1 version associated less efficiently with spindle microtubules. Consistently, reducing the level of augmin in Cytosim simulations largely recapitulated the spindle phenotypes observed incycb3;1andcdkb1mutants. Our results emphasize the importance of cell cycle-dependent phospho-control of the mitotic spindle in plant cells. They also support the validity of our computational model as a framework for the exploration of mechanisms controlling the organization of the spindle in plants and in other species.
Unearthing historical insights: semantic organization and application of historical newspapers from a fine-grained knowledge element perspective
Authors: Sun Shaodan , Deng Jun , Qin Xugong 
Published: November 2023

PurposeThis paper aims to amplify the retrieval and utilization of historical newspapers through the application of semantic organization, all from the vantage point of a fine-grained knowledge element perspective. This endeavor seeks to unlock the latent value embedded within newspaper contents while simultaneously furnishing invaluable guidance within methodological paradigms for research in the humanities domain.Design/methodology/approachAccording to the semantic organization process and knowledge element concept, this study proposes a holistic framework, including four pivotal stages: knowledge element description, extraction, association and application. Initially, a semantic description model dedicated to knowledge elements is devised. Subsequently, harnessing the advanced deep learning techniques, the study delves into the realm of entity recognition and relationship extraction. These techniques are instrumental in identifying entities within the historical newspaper contents and capturing the interdependencies that exist among them. Finally, an online platform based on Flask is developed to enable the recognition of entities and relationships within historical newspapers.FindingsThis article utilized the Shengjing Times·Changchun Compilation as the datasets for describing, extracting, associating and applying newspapers contents. Regarding knowledge element extraction, the BERT + BS consistently outperforms Bi-LSTM, CRF++ and even BERT in terms of Recall and F1 scores, making it a favorable choice for entity recognition in this context. Particularly noteworthy is the Bi-LSTM-Pro model, which stands out with the highest scores across all metrics, notably achieving an exceptional F1 score in knowledge element relationship recognition.Originality/valueHistorical newspapers transcend their status as mere artifacts, evolving into invaluable reservoirs safeguarding the societal and historical memory. Through semantic organization from a fine-grained knowledge element perspective, it can facilitate semantic retrieval, semantic association, information visualization and knowledge discovery services for historical newspapers. In practice, it can empower researchers to unearth profound insights within the historical and cultural context, broadening the landscape of digital humanities research and practical applications.
How does frugality influence customer complaints through the mediation of the zone of tolerance? Empirical evidence from Chinese consumers purchasing Shanzhai products
Authors: Lin Ming , Tian Mu , Wang Yifan , Shu Libing 
Published: November 2023

PurposeThis study aims to investigate whether frugality increases customer complaints via the mediating role of the zone of tolerance (ZOT) of quality for Shanzhai products. Additionally, the study seeks to explore how customer empathy as a boundary condition affects the relationship between the ZOT of quality and customer complaints.Design/methodology/approachBased on the purchasing data of 241 low-income customers from business-to-consumer (B2C) e-commerce and social platforms in China, hierarchical analysis was conducted in the study. Moreover, “PROCESS SPSS Macro” was used to test the mediation effect of the ZOT of quality and the moderated mediation effect with customer empathy as a moderator.FindingsThe results indicate a positive impact of frugality on customer complaints, which is mediated by the ZOT of quality. Moreover, with an increase in customer empathy, the negative effect of the ZOT of quality on customer complaints increases, and the positive influence of frugality on customer complaints, through the mediation of the ZOT of quality, also becomes stronger.Originality/valueDrawing upon the principle of cognitive consistency, this study not only extends the stream of research on frugality by exploring how frugality influences customer complaints via the mediation of the ZOT of quality but also sheds new light on the research on customer empathy by exploring its moderating role in the relationship between the ZOT of quality and customer complaints.
Employee and manager's emotional intelligence and individual adaptive performance: the role of servant leadership climate
Authors: Balti Mariem , Karoui Zouaoui Samia 
Published: November 2023

PurposeThe present research attempts to show the contribution of emotional intelligence and servant leadership climate to individual adaptive performance. The authors intend to explain the relations between the emotional intelligence of employees as well as of manager and the employee's adaptive performance. Moreover, this research assesses the significance of the mediating role of “servant leadership” climate in the relationship between the emotional intelligence of the manager and the employee's adaptive performance.Design/methodology/approachThis research uses the quantitative research method and is included in explanatory research. Data collection used several informants for each organization. Data were collected using a sample of 57 managers and 204 team members spread over 24 companies belonging to different sectors of activity.FindingsEmployees' emotional intelligence directly influences individual adaptive performance. There is a direct influence of manager's emotional intelligence on individual adaptive performance. Then, there is an indirect influence of emotional intelligence on individual adaptive performance through the mediation of servant leadership climate.Originality/valueThe novelty of this research is in its effort to observe the multilevel mediation of servant leadership climate with other variables developed in the research model. No previous studies have found a relationship between employee and manager's emotional intelligence and individual adaptive performance.
Foreign market involvement, entry-mode learning potential and SME internationalization outcomes
Authors: Elbanna Said , Hsieh Linda , Child John , Narooz Rose , Marinova Svetla , Puthusserry Pushyarag , Karmowska Joanna , Tsai Terence , Zhang Yunlu 
Published: November 2023

PurposeDrawing on an organizational learning perspective, this paper examines the effect of levels of foreign market involvement (intensity and geographic spread) on internationalization outcomes recognizing that the moderating influence of entry-mode learning potential is not well documented in the literature on small- and medium-sized enterprises (SMEs).Design/methodology/approachThe sample includes 180 SMEs evenly selected from three industries: biotechnology, software and clothing (60 firms in each industry). The sampled firms employ less than 250 employees and are equally distributed between three developed economies and three emerging economies. All were engaged in foreign business.FindingsThe authors find that there is a direct relationship between levels of foreign market involvement and internationalization outcomes. Entry-mode learning potential moderates the relationship between intensity of foreign market involvement and internationalization outcomes but not the relationship between geographic spread and internationalization outcomes.Practical implicationsThis study reveals several new insights that help explain the pathway through which foreign market involvement activities are translated into internationalization outcomes.Originality/valueThe authors conclude that the positive relationship between intensity of foreign market involvement and internationalization outcomes is strengthened when SMEs also use an entry mode with a higher learning potential than exporting only.
Decoupling of body size, physiological state and subsequent performance in a developmentally manipulated songbird
Authors: Allen Joshua M. , Hodinka Brett L. , Hall Hannah M. , Leonard Kathryn M. , Barbera Raven A. , Desjardin Genavieve C. , Williams Tony D. 
Published: November 2023

Abstract

It is generally assumed that larger juveniles are more physiologically mature, hence their overall condition and subsequent performance are higher.

Some taxa face extreme workload transitions during development (e.g. nest departure in birds), which may select for non‐linear growth profiles that decouple relationships between body size and physiological state, making the links between metrics of development (e.g. body mass) and juvenile performance uncertain.

We manipulated perceived mass in European starlings approaching fledging using 4.0 g weighted backpacks, measuring subsequent growth trajectories (mass, wing length) and physiological state (aerobic capacity, energy state, oxidative status) to test whether body size and physiology are coupled during a non‐linear developmental phase (pre‐fledging mass recession). Nanotag radio transmitters were then used to track post‐fledging performance (activity, activity‐slope, dispersal).

Weighted nestlings had greater pre‐fledging mass recession and marginally reduced wing growth when compared to controls. Consequently, weighted birds had lower body mass at fledging, while also displaying markers of oxidative stress (increased reactive oxygen metabolites), yet aerobic capacity and energy state were equal between treatment groups.

Previously weighted birds showed no correlation between fledgling state (body size, physiology) and subsequent performance. However, control fledglings that had decreased mass recession and greater wing growth prior to fledging dispersed earlier, while longer wings at fledging were associated with more rapid increases in post‐fledging activity (activity‐slope). In addition, control fledglings with lower haemoglobin also dispersed earlier, while increased reactive oxygen metabolites were associated with decreased activity levels. Body mass and energy state were unrelated to any metric of post‐fledging performance.

Our data suggest mass‐independent development of some physiological traits in species with non‐linear growth profiles, potentially with a context‐dependent oxidative cost, and that physiology may predict some measures of post‐fledging performance not captured by body size.

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Wide divergence of fungal communities inhabiting rocks and soils in a hyper‐arid Antarctic desert
Authors: Canini Fabiana , Borruso Luigimaria , Newsham Kevin K. , D'Alò Federica , D'Acqui Luigi P. , Zucconi Laura 
Published: November 2023

AbstractHighly simplified microbial communities colonise rocks and soils of continental Antarctica ice‐free deserts. These two habitats impose different selection pressures on organisms, yet the possible filtering effects on the diversity and composition of microbial communities have not hitherto been fully characterised. We hence compared fungal communities in rocks and soils in three localities of inner Victoria Land. We found low fungal diversity in both substrates, with a mean species richness of 28 across all samples, and significantly lower diversity in rocks than in soils. Rock and soil communities were strongly differentiated, with a multinomial species classification method identifying just three out of 328 taxa as generalists with no affinity for either substrate. Rocks were characterised by a higher abundance of lichen‐forming fungi (typically Buellia, Carbonea, Pleopsidium, Lecanora, and Lecidea), possibly owing to the more protected environment and the porosity of rocks permitting photosynthetic activity. In contrast, soils were dominated by obligate yeasts (typically Naganishia and Meyerozyma), the abundances of which were correlated with edaphic factors, and the black yeast Cryomyces. Our study suggests that strong differences in selection pressures may account for the wide divergences of fungal communities in rocks and soils of inner Victoria Land.
Active, assertive, anointed, absconded? Testing claims about career politicians in the United Kingdom
Authors: WARNCKE PHILIP , SEARING DONALD D. , ALLEN NICHOLAS 
Published: November 2023

AbstractThis article undertakes a comprehensive investigation into several common critiques of career politicians. Career politicians are said to be self‐serving: active and assertive when it suits their career interests, and much more interested in attaining higher offices than in serving as constituency‐oriented MPs. Yet, empirical investigations of their alleged behaviours are few, and the results are patchy and mixed. Focusing on the United Kingdom case and using a multi‐dimensional conceptualization that accords with academic and popular understandings of career politicians, the article draws on uniquely rich attitudinal and longitudinal behavioural data covering the first large generational wave of career politicians to be elected to parliament in the early 1970s. It reports findings consistent with contemporary critiques, suggesting that such dispositions are inherent in the role of career politician. The strongest career politicians among this first wave concentrated strategically on career‐serving activities, voted strategically to safeguard their careers, attained and retained successfully ministerial offices and prioritized their personal goals over their party obligations. The article further demonstrates that different measures used by researchers can produce contradictory results and that future comparative research should seek to range beyond unidimensional indicators.
Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection
Authors: Cho Se‐Young , Vaidya Bipin , Kwon Hyuk‐Jung , Kim Eunsun , Kim Youngsoon , Choi Ji Yeong , Seo Hyomin , Kwon Joseph , Kim Duwoon 
Published: November 2023

AbstractOryctes rhinoceros nudivirus (OrNV) infects the larval stage of many coleopteran insects; however, the underlying mechanisms and biomarkers of infection are not fully characterised. In this study, an optimal culture condition was developed for OrNV replication and proteomic biomarkers were identified using comparative proteomic analysis. The highest level of viral copy number was observed in Sf9 cells treated with 450 μM of H2O2 and 2% foetal bovine serum (FBS). Among the 48 identified proteins, 14 proteins were significantly modulated in 2% FBS and H2O2‐ treated OrNV‐infected cells (F2V) as compared with 10% FBS treated non‐infected cells (F10M). Network analysis revealed that SLC25A5, VDAC3, PHB2, and ANXA1 act as signature proteins for OrNV replication. Moreover, viral envelope glycoproteins, GRBNV_gp28‐like and GrBNV_gp62‐like proteins could be used as sensitive diagnostic signatures for OrNV infection. Furthermore, to conveniently identify the OrNV‐infection in Allomyrina dichotoma larvae, an image classification model was trained by Google Teachable Machine, which distinguished images with accuracy rates of 91% and 86% for infected and non‐infected larvae, respectively, at a learning rate of 0.001. This study demonstrated that Sf9 cell medium treated with 2% FBS and 450 μM H2O2 is a permissible culture condition for OrNV replication. Proteomic signatures may be involved in the progression of viral infection. Additionally, a low‐cost and non‐invasive machine learning‐derived digital imaging analysis may improve the prediction of OrNV infection in larvae.
Diagnostic delay and its associated factors in Chinese axial spondyloarthritis: A single‐center study of 1295 patients
Authors: Zheng Xuqi , Chen Zena , Wu Xinyu , Xie Ya , Wu Jialing , Xiao Min , Yang Mingcan , Tu Liudan , Cao Shuangyan , Wei Qiujing , Gu Jieruo 
Published: November 2023

AbstractAimTo delineate the landscape of diagnostic delay in Chinese axial spondyloarthritis (axSpA), investigate its associated factors, and explore its potential impact on medication modalities.MethodsA total of 1295 patients fulfilling the ASAS classification criteria were obtained. Demographic and clinical data were collected through face‐to‐face interviews, based on predesigned questionnaires and available medical records. Logistic regression analyses under univariate and multivariable model were performed, using the median of diagnostic delay as the cut‐off point for group classification. Differences between early‐ and late‐diagnosed groups were subsequently compared by the Pearson chi‐square test or Mann–Whitney U test.ResultsOf 1295 axSpA patients, 80.3% were male and the median of disease duration was 8.0 years. The median (IQR) diagnostic delay in Chinese axSpA was 3.0 (1.0 ~ 7.0) years and 24.8% of them reported a history of misdiagnosis. Older age at onset (OR = 0.97, p < .001) and higher education attainment (p = .001) were correlated with early diagnosis of axSpA, whereas coming from less developed areas (p = .002), a history of peripheral arthritis at the time of diagnosis (OR = 1.58, p = .002) and history of misdiagnosis (OR = 1.98, p < .001) increased the risk of diagnostic delay. Oral medication modalities were similar between two groups, but the proportion with no medication ever was higher in the late‐diagnosed group (26.5% vs. 20.7%, p = .02).ConclusionOur findings depicted a detailed spectrum of diagnostic delay in Chinese axSpA, verified five associated factors that may help facilitate timely diagnosis of axSpA, and pinpointed that timely medication was unsatisfying, especially in the late diagnosis group.
Flower/testa colour associations with grain yield, quality and tolerance to seed weevil in grass pea phenotypes
Authors: Tsialtas Ioannis T. , Irakli Maria 
Published: November 2023

AbstractGrass pea (Lathyrus sativus L.) is considered an orphan/underutilised grain legume with high yield and nutritive potential. It is variable in flower and seed coat (testa) colour and this variation could possibly be used as a biomarker in phenotypic selection for yield and quality traits. Three phenotypes differing in flower/testa colour (white flower‐white to creamy testa and white hilum [TWHW], slightly blue banner and wing colouration‐white to creamy testa and coloured halo around hilum [TWHC] and blue banner and wing colouration‐spotted testa and coloured halo around hilum [TSHC]) were tested over two growing seasons, under rainfed, Mediterranean conditions. They differed only in the number of seeds per pod; the phenotype TWHW had the lowest number (2.2 seeds pod−1). The seed yield per plant (SY) was highly correlated with the number of pods per plant and was higher under wetter and warmer conditions during the reproductive period (April–May). The three phenotypes showed no difference in tolerance against bruchid (Bruchus tristiculus) infection (F2,8 = 0.14, p > .05), which was higher in the most productive growing season (6.44%, F1,4 = 14.8, p < .05). In the phenotype TWHW, three seed classes (intact, bruchid‐damaged, parasitoid‐damaged seeds) were tested for SY, quality traits and tolerance to bruchids. Though insect damaged seeds showed significant reduction in emergence rates (from −57% up to −91%), there were no differences in SY (19.11–24.53 g plant−1, F2,8 = 0.86, p > .05) and yield components (seeds per pod, 1000‐seed weight, number of pods per plant). However, seeds of plants derived from parasitoid‐damaged seeds had higher concentration of the neurotoxin ODAP (4.92 mg g−1, F2,8 = 8.76, p < .01). On the other hand, seeds of plants derived from bruchid‐damaged seeds showed higher percentage of damaged seeds by the endoparasitoid Triaspis thoracica (2.25% vs. 0.67%–1.00%, F2,8 = 5.47, p < .05). Concluding, flower and testa colour cannot be used as biomarkers of seed yield and quality in grass pea, but it seemed that a tripartite interaction (grass pea–bruchid–endoparasitoid) may occur that affects ODAP concentration and the susceptibility of seeds to insect damages.