bluuebunny/crossref_metadata_2025_split huggingface dataset:
The dopamine D2 receptors antagonist Veralipride inhibits carbonic anhydrases: solution and crystallographic insights on human isoforms
Authors: Angeli Andrea , Ferraroni Marta , Capasso Clemente , Supuran Claudiu T. 
Published: February 2024

AbstractThe inhibitory effects of veralipride, a benzamide‐class antipsychotic acting as dopamine D2 receptors antagonist incorporates a primary sulfonamide moiety and was investigated for its interactions with carbonic anhydrase (CA) isoforms. In vitro profiling using the stopped‐flow technique revealed that veralipride exhibited potent inhibitory activity across all tested hCA isoforms, with exception of hCA III. Comparative analysis with standard inhibitors, acetazolamide (AAZ), and sulpiride, provided insights for understanding the relative efficacy of veralipride as CA inhibitor. The study reports the X‐ray crystal structure analysis of the veralipride adduct with three human (h) isoforms, hCA I, II, and CA XII mimic, allowing the understanding of the molecular interactions rationalizing its inhibitory effects against each isoform. These findings contribute to our understanding of veralipride pharmacological properties and for the design of structural analogs endowed with polypharmacological properties.
Neural correlates of audiovisual narrative speech perception in children and adults on the autism spectrum: A functional magnetic resonance imaging study
Authors: Ross Lars A. , Molholm Sophie , Butler John S. , Del Bene Victor A. , Brima Tufikameni , Foxe John J. 
Published: February 2024

AbstractAutistic individuals show substantially reduced benefit from observing visual articulations during audiovisual speech perception, a multisensory integration deficit that is particularly relevant to social communication. This has mostly been studied using simple syllabic or word‐level stimuli and it remains unclear how altered lower‐level multisensory integration translates to the processing of more complex natural multisensory stimulus environments in autism. Here, functional neuroimaging was used to examine neural correlates of audiovisual gain (AV‐gain) in 41 autistic individuals to those of 41 age‐matched non‐autistic controls when presented with a complex audiovisual narrative. Participants were presented with continuous narration of a story in auditory‐alone, visual‐alone, and both synchronous and asynchronous audiovisual speech conditions. We hypothesized that previously identified differences in audiovisual speech processing in autism would be characterized by activation differences in brain regions well known to be associated with audiovisual enhancement in neurotypicals. However, our results did not provide evidence for altered processing of auditory alone, visual alone, audiovisual conditions or AV‐ gain in regions associated with the respective task when comparing activation patterns between groups. Instead, we found that autistic individuals responded with higher activations in mostly frontal regions where the activation to the experimental conditions was below baseline (de‐activations) in the control group. These frontal effects were observed in both unisensory and audiovisual conditions, suggesting that these altered activations were not specific to multisensory processing but reflective of more general mechanisms such as an altered disengagement of Default Mode Network processes during the observation of the language stimulus across conditions.
Circular supply chains in manufacturing—Quo vadis? Accomplishments, challenges and future opportunities
Authors: Bhattacharya Arijit , Srivastava Shefali , Majumdar Abhijit 
Published: February 2024

AbstractCircular approach in manufacturing supply chain (SC) operations yields multiple benefits through optimal utilisation and consumption of resources. This study maps the scope and structure of circularity in the manufacturing SC discipline and explores the evolution of the domain over time. We review 946 journal articles published between 2013 and September 2023. Our study identifies key drivers and barriers to circular economy (CE) deployment in manufacturing SC operations, bibliometric parameters, emerging research themes, decision support tools, theories and applications. Using the theory extension approach, we propose a strategic framework to fortify the deployment of circularity in SCs. This comprehensive study renders a methodological contribution through combined descriptive content analysis and bibliometric and network analyses to evaluate the circular manufacturing SC operations concepts, theories and applications. We posit that manufacturing firms require to deploy innovation‐led approaches to embed the CE strategies in their SC operations. We find that the studies investigating green skill development and circularity‐culture adoption can facilitate manufacturers to understand the efficacy of circularity in their SC operations. The findings of this study can facilitate the practitioners to identify the links between the CE approaches and their strategic implications and examine CE implementation at the strategic level.
Exploring Culture Media Diversity to Produce Fungal Secondary Metabolites and Cyborg Cells
Authors: Geris Regina , Teles de Jesus Vitória Evelyn , Ferreira da Silva Antonio , Malta Marcos 
Published: February 2024

AbstractFungi are microorganisms of significant biotechnological importance due to their ability to provide food and produce several value‐added secondary metabolites and enzymes. Its products move billions of dollars in the pharmaceutical, cosmetics, and additives sectors. These microorganisms also play a notable role in bionanotechnology, leading to the production of hybrid biological‐inorganic materials (such as cyborg cells) and the use of their enzyme complex in the biosynthesis of nanoparticles. In this sense, optimizing the fungal growth process is necessary, with selecting the cultivation medium as one of the essential factors for the microorganism to reach its maximum metabolic expression. The culture medium‘s composition can also impact the nanomaterial's stability and prevent the incorporation of nanoparticles into fungal cells. Therefore, our main objectives are the following: (1) compile and discuss the most commonly employed culture media for the production of fungal secondary metabolites and the formation of cyborg cells, accompanied by preparation methods; (2) provide a six‐step guide to investigating the fungal metabolomic profile and (3) discuss the main procedures of microbial cultivation to produce fungal cyborg cells.
Hernandulcin Production in Elicited Hairy Roots of Phyla scaberrima: Toward Sustainable Production of a Non‐Caloric Sweetener with Nutraceutical Properties
Authors: Castro‐Juárez Carlos Jonnathan , Luna‐Suárez Silvia , de Fátima Rosas‐Cárdenas Flor , Villa‐Ruano Nemesio 
Published: February 2024

AbstractHerein we report on the generation of hairy root lines of P. scaberrima able to produce hernandulcin (HE), a non‐caloric sweetener with nutraceutical properties. From ten different lines analyzed, three synthesized up to 100 mg ⋅ L−1 HE under the batch culture conditions standardized in this investigation. Adding elicitors (salicylic acid, chitin, Glucanex, polyethylene glycol) and biosynthetic precursors (farnesol and (+)‐epi‐alpha‐bisabolol) significantly altered HE accumulation. Chitin and Glucanex enhanced HE production from 130 to 160 mg ⋅ L−1, whereas farnesol and (+)‐epi‐alpha‐bisabolol from 165 to 200 mg ⋅ L−1without dependence on biomass accumulation. Improved batch cultures containing liquid Murashige & Skoog medium (MS; pH 7), added with 4 % sucrose, 0.5 mg ⋅ L−1 naphthaleneacetic acid, 100 mg ⋅ L−1 Glucanex, 150 mg ⋅ L−1 chitin, 250 mg ⋅ L−1 farnesol, and 150 mg ⋅ L−1 (+)‐epi‐alpha‐bisabolol at 25 °C (12 h light/12 h darkness), triggered HE accumulation to 250 mg ⋅ L−1 in 25 days. The efficiency of each recombinant line is discussed.
The resiliency of urban community college students: Implications for practice
Authors: Campagna Michele 
Published: February 2024

AbstractCommunity colleges are vital sources of educational and economic opportunities in urban areas. This chapter provides readers with a review of the research on students served by these institutions. While the experiences of this population are similar in many ways to those of community college students across the country, the influence of their urban environment makes their lived experiences quite distinct. This chapter highlights that although a great deal of emphasis in the literature has been placed on the economic, educational, personal, and social struggles faced by urban community college students, resiliency is also a prominent positive characteristic of this population. The chapter concludes with recommendations for evidence‐based strategies that affirm these students’ cultural capital and are proven to be effective approaches to enhancing the resiliency and success of urban community college students.
How long will it take? Commuting time and differential access to an urban community college
Authors: Cortes Juan B. , Smith‐Onyewu Zyrashae , Kim‐Christian Paula , Dewey Nathaniel , Stein Marc L. 
Published: February 2024

AbstractIn this chapter, we examine how spatial access, the extent to which individuals must traverse space or time to reach a community college, varies by time of day across socio‐demographic groups. We specifically measure spatial access as the time required to travel to a community college by public transit, a common mode of transportation for many urban residents. Our findings revealed substantive variation in travel times between socio‐demographic groups suggesting varying levels of spatial access. They also highlight how public transit infrastructure, residential patterns, and community college locations interact to influence how long students must travel to access postsecondary opportunities.
Vascular Ehlers‐Danlos syndrome: A null COL3A1 variant found in a patient with loin pain without marked cutaneous features (case report)
Authors: Subramaniam Shreenidhi Ranganatha , Yeung Lam Fung , Choy L. Y. Lois , Kwok Jeffrey Sung Shing 
Published: February 2024

Key Clinical MessagePatients with null variants may have milder vascular Ehlers‐Danlos syndrome, presenting with seemingly non‐specific complaints and subtle cutaneous features that may be missed. A high index of suspicion and early genetic testing (aided by next‐generation sequencing) were crucial for prevention of life‐threatening complications in the patient and family members.
In‐Situ Characterization Technologies for Electrocatalytic Reduction nitrate to Ammonia on Copper‐Based Catalysts
Authors: Fu Chunhong , Sun Jiangli , Du Yingying , Zhou Minghua 
Published: February 2024

AbstractThe excess nitrate (NO3−) in water mainly comes from agricultural fertilization and industrial wastewater, which breaks the nitrogen balance and poses a serious threat to the environment and human health. Driven by renewable energy, the electrocatalytic NO3− reduction to ammonia (NH3) (ENO3RA) is an environmentally friendly and sustainable technology. Due to its special structure, copper (Cu) is currently one of the best catalysts for ENO3RA, but the reaction mechanism and the structure–activity relationships of catalysts are still not clear enough. In‐situ characterization is a powerful tool to gain insight into the reaction process. This review introduces several types of in‐situ techniques such as in‐situ XAS, in‐situ FTIR and in‐situ DEMS, summarizes five pathways for converting *NO as the key intermediate to NH3 during ENO3RA on Cu‐based catalysts. The research progress of Cu‐based electrocatalysts in recent years is sorted out from the aspects of composition and structure, and the catalytic mechanisms are discussed with the help of in‐situ characterization technologies. This review would be of help to provide reference characterization methods for exploring the mechanism and the design of electrocatalysts for ENO3RA.
Affine combination‐based over‐sampling for imbalanced regression
Authors: Li Zhen‐Zhen , Huang Niu , Yi Lun‐Zhao , Fu Guang‐Hui 
Published: February 2024

AbstractImbalanced domain prediction analysis is currently one of the hot research topics. Many real‐world data mining analyses involve using imbalanced data to obtain predictive models. In the context of imbalance, research on classification problems has been extensive, but research on regression problems is negligible. Rare values rarely occur in imbalanced regression problems, but the focus is on accurately predicting the continuous target variables of rare instances. One of the challenges in imbalanced regression is finding a suitable strategy to rebalance the original dataset in order to improve the predictive performance of the model in rare instances. In this study, two algorithms are proposed: sigma nearest over‐sampling based on convex combination for regression (SNOCCR) and affine combination‐based over‐sampling (ACOS). ACOS rebalances the original dataset by generating new instances through the affine combinations of the original examples. The region where the new instances are generated can be adjusted based on the distribution of the data, ensuring that the generated cases better mimic the distribution of the original examples. The comparison among ACOS, SNOCCR, and other preprocessing methods was conducted on 15 datasets to validate the predictive performance of models trained on rebalanced datasets for rare instances. The experimental results indicate that ACOS outperforms other existing methods.
Challenges for the Discovery of Non‐Covalent WRN Helicase Inhibitors
Authors: Heuser Alisa , Abdul Rahman Wassim , Bechter Elisabeth , Blank Jutta , Buhr Sylvia , Erdmann Dirk , Fontana Patrizia , Mermet‐Meillon Fanny , Meyerhofer Marco , Strang Ross , Schrapp Maxime , Zimmermann Catherine , Cortes‐Cros Marta , Möbitz Henrik , Hamon Jacques 
Published: February 2024

AbstractThe Werner Syndrome RecQ helicase (WRN) is a synthetic lethal target of interest for the treatment of cancers with microsatellite instability (MSI). Different hit finding approaches were initially tested. The identification of WRN inhibitors proved challenging due to a high propensity for artefacts via protein interference, i. e., hits inhibiting WRN enzymatic activities through multiple, unspecific mechanisms. Previously published WRN Helicase inhibitors (ML216, NSC19630 or NSC617145) were characterized in an extensive set of biochemical and biophysical assays and could be ruled out as specific WRN helicase probes. More innovative screening strategies need to be developed for successful drug discovery of non‐covalent WRN helicase inhibitors.
Apparent absence of hypothalamic cholinergic neurons in the common ostrich and emu: Implications for global brain states during sleep
Authors: Mazengenya Pedzisai , Lesku John A. , Rattenborg Niels C. , Manger Paul R. 
Published: February 2024

AbstractWe examined the presence/absence and parcellation of cholinergic neurons in the hypothalami of five birds: a Congo grey parrot (Psittacus erithacus), a Timneh grey parrot (P. timneh), a pied crow (Corvus albus), a common ostrich (Struthio camelus), and an emu (Dromaius novaehollandiae). Using immunohistochemistry to an antibody raised against the enzyme choline acetyltransferase, hypothalamic cholinergic neurons were observed in six distinct clusters in the medial, lateral, and ventral hypothalamus in the parrots and crow, similar to prior observations made in the pigeon. The expression of cholinergic nuclei was most prominent in the Congo grey parrot, both in the medial and lateral hypothalamus. In contrast, no evidence of cholinergic neurons in the hypothalami of either the ostrich or emu was found. It is known that the expression of sleep states in the ostrich is unusual and resembles that observed in the monotremes that also lack hypothalamic cholinergic neurons. It has been proposed that the cholinergic system acts globally to produce and maintain brain states, such as those of arousal and rapid‐eye‐movement sleep. The hiatus in the cholinergic system of the ostrich, due to the lack of hypothalamic cholinergic neurons, may explain, in part, the unusual expression of sleep states in this species. These comparative anatomical and sleep studies provide supportive evidence for global cholinergic actions and may provide an important framework for our understanding of one broad function of the cholinergic system and possible dysfunctions associated with global cholinergic neural activity.
Polycyclic Heteroaromatic π‐Linkers Provide Dithienylethene Switches with Favorable Thermal and Photochemical Properties for Solar‐Energy Storage
Authors: S Thillaiarasi , Perumalla D. Sravanakumar , Oruganti Baswanth , Durbeej Bo 
Published: February 2024

AbstractDithienylethene photoswitches with an aromatic π‐linker as the bridge between the two thiophene units are attractive starting materials for developing molecular solar thermal energy (MOST) storage systems, partly because the aromaticity of their ring‐open forms is a favorable feature with regard to the energy‐storage densities of their ring‐closed forms produced by photoinduced electrocyclization (photocyclization) reactions. At the same time, this typically leads to small barriers for their thermal cycloreversion reactions, which are not desirable in this context. Here, we use computational methods to show that this problem can be circumvented with polycyclic heteroaromatic π‐linkers. Specifically, through the tuning of the aromatic character of the individual rings of such a π‐linker (like indole or isoindole), it is shown to be possible to strike a delicate balance between the seemingly contrasting requirements of simultaneously achieving both a high energy‐storage density and a large cycloreversion barrier. Furthermore, this design is also found to provide for a quick and efficient photocyclization reaction, owing to the onset of excited‐state antiaromaticity in the π‐linker upon light absorption of the ring‐open form. Altogether, dithienylethenes with polycyclic heteroaromatic π‐linkers appear to have both thermal and photochemical properties suitable for further development into future MOST systems.
Gypsum lakes, sandflats and soils revealed from the Triassic Red Peak Formation of the Chugwater Group, north‐central Wyoming
Authors: Bradford Maya Yamei , Benison Kathleen C. 
Published: February 2024

AbstractBedded gypsum is relatively common in bedded evaporites associated with red bed siliciclastics of Permo‐Triassic Pangea. However, little attention has been paid to the textures of ancient gypsum, which can be used to refine interpretations of depositional environment and diagenetic history. This project describes the textures of bedded gypsum from an outcrop of the Triassic Red Peak Formation (Chugwater Group) near Greybull, Wyoming. Fieldwork, petrography and X‐ray diffraction reveal three distinct lithologies of bedded gypsum: bottom‐growth gypsum, laminated gypsum and clastic gypsum. Bottom‐growth gypsum precipitated at the bottom of shallow saline surface water bodies. Laminated gypsum probably formed in shallow saline lakes and mudflats. Clastic gypsum units are composed of aeolian‐reworked bottom‐growth gypsum crystals deposited in sandflats. Red siliciclastic mudstones are characterised by their massive nature and abundant blocky peds. Detailed study of this outcrop of the Red Peak Formation shows that it formed in shallow saline lakes and associated mudflats, sandflats and desert soils.
Resource instability undermines predictable plasticity‐mediated morphological responses to diet in a postglacial fish
Authors: Koene J. Peter , Adams Colin E. 
Published: February 2024

AbstractPhenotypic plasticity has been presented as a potential rapid‐response mechanism with which organisms may confront swift environmental change and increasing instability. Among the many difficulties potentially facing freshwater fishes in recently glaciated ecosystems is that of invertebrate prey communities becoming significantly altered in species composition and relative abundance. To test how the rapidity of diet resource change may affect phenotypic responses during development, we subjected juvenile brown trout to pelagic‐type or littoral‐type diets that alternated either daily, sub‐seasonally, or not at all over a single growth season. The proportional intake of each diet was traced with stable isotopes of carbon and nitrogen and modelled with morphometric data on head and jaw shape. While those trout exposed to a single diet type developed predictable morphologies associated with pelagic or littoral foragers, those raised on alternating diets expressed more unpredictable morphologies. With extreme (daily) or even sub‐seasonal (monthly) resource instability, the association of diet type with the phenotype was overwhelmed, calling into question the efficacy of plasticity as a means of adaptation to environments with rapidly fluctuating prey resources.
Contextualizing and generalizing drivers and barriers of urban living labs for climate resilience
Authors: Quadros Aniche Laura , Edelenbos Jurian , Gianoli Alberto , Caruso Rochelle , DeLosRíos‐White Marta Irene , Pyl Wissink‐Nercua Charmae , Undabeitia Asier , Enseñado Elena Marie , Gharbia Salem 
Published: February 2024

AbstractUrban Living Labs are open innovation ecosystems that integrate research and innovation activities within urban communities. However, while solutions co‐created and tested in the Urban Living Labs must be contextualized and tailored to each city's uniqueness, broader impact requires generalization and systematic replication across geographical, institutional, and sectoral boundaries. This article examines nine Living Labs in European coastal cities, identifying several barriers and drivers for mainstreaming and upscaling solutions to increase climate resilience through the Living Lab Integrative Process. Our analysis focuses on three main categories. First, social and cultural aspects highlighted include stakeholder engagement and awareness, communication, and dissemination. Second, we assess institutional and political aspects, such as silos, bureaucracy, and resources. Last, we investigate technical factors as knowledge and experience, technical and internal capacity, data availability and accessibility, climate‐related policies and actions, and long‐term perspective. The results suggest that while some barriers and drivers are common across the cases, providing generalizable patterns, there are also specific differences requiring tailored solutions at the local scale. Nonetheless, the diversity in drivers indicates the potential for sharing knowledge across cases to translate, embed, and scale solutions, enhancing the transition toward climate resilience. Learning and innovation in real‐life contexts are fundamental in the Living Lab approach, and our findings demonstrate that cross‐case learning can enhance an iterative process of contextualizing and generalizing innovative climate solutions.
Regulation of autoimmune‐mediated neuroinflammation by endothelial cells
Authors: Kallal Neil , Hugues Stephanie , Garnier Laure 
Published: February 2024

AbstractThe CNS has traditionally been considered an immune‐privileged organ, but recent studies have identified a plethora of immune cells in the choroid plexus, meninges, perivascular spaces, and cribriform plate. Although those immune cells are crucial for the maintenance of CNS homeostasis and for neural protection against infections, they can lead to neuroinflammation in some circumstances. The blood and the lymphatic vasculatures exhibit distinct structural and molecular features depending on their location in the CNS, greatly influencing the compartmentalization and the nature of CNS immune responses. In this review, we discuss how endothelial cells regulate the migration and the functions of T cells in the CNS both at steady‐state and in murine models of neuroinflammation, with a special focus on the anatomical, cellular, and molecular mechanisms implicated in EAE.