Dietary Mango Ginger May Enhance the Exercise Performance and Reduces Lipid Profile when Combined with Treadmill Running in a Rat Model
Authors: Sahin Kazim , Tuzcu Mehmet , Orhan Cemal , Sahin Nurhan , Akdemir Fatih , Pala Ragip , Juturu Vijaya 
Published: November 2023

ObjectiveMango ginger (MG) are the major pungent, naturally occurring active compounds in MGicum fruits such as hot chili peppers with the most abundant forms being MGaicin, dihydroMGaicin, and nordihydroMGaicin. We recently demonstrated that MG Mango Ginger down regulated VCAM‐1. Therefore, this study is to investigate the Mango ginger (MG) supplementation on exercise (Ex) performance and changes in serum parameters and muscle and aorta NF‐κB, Nrf2 and HO‐1 levels and aorta Nox2, Nox4 and p47phox in rats after exhaustive Ex.MethodsEight week old male Wistar rats were treated with four treatment groups (i) control [no Ex, Group I (CTL)] (ii) CTL + MG [no Ex, 50 mg/kg mg MG, Group II] (iii) CTL + Ex [Group III] and (iv) CTL + Ex+ MG [Ex, 50 mg/kg mg MG, Group IV] administered daily for 8 wks. The Ex protocols were performed on a motor‐driven rodent treadmill (TMR). The animals in the chronic Ex groups were habituated by treadmill Ex over a 5‐d period such as: 1st day 10 m/min, 10 min, 2nd day 20 m/min; 10 min, 3rd day 25 m/min, 10 min, 4th day 25 m/min, 20 min and 5th day 25 m/min, 30 min. Animals were exercised at 25 m/min, 45 min/d, 5 d/week for 8 wks. Blood analysis for triglycerides (TG) and cholesterol (CHOL), muscle analysis for lactate, muscle antioxidant capacity and muscle oxidative stress (OS) were estimated and protein in the gastrocnemius muscle and aorta was evaluated in each group of rats by Western blotting.ResultsAs shown in Table, Group IV significantly increased running performance and exhaustion time. In Group IV there was a significant decrease in TG and CHOL were observed compared with other treatments. A significant decrease in lactate, muscle oxidative stress, and increase in muscle antioxidant activity were observed in Group IV. Muscle and aorta protein levels of nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2), and heme oxygenase‐1 (HO1) increased and a decrease in nuclear factor (NF‐κB) in muscle and aorta and Nox2, Nox4 and p47phox in aorta were observed in Group IV. There were no significant differences in any of the end points in Group I and II. No significant changes in liver and kidney functions were observed in any of the treated groups.ConclusionThe present data suggested that dietary MG enhances running performance and decreased OS. These results suggest MG with Ex may enhance the effect of exercise by improving antioxidant activity and reducing OS.Support or Funding InformationOmniActive Health Technologies Ltd. India
The effects of mango ginger on plasma biochemical parameters in exercise‐trained rats

Item
Groups
SEM
‐‐P‐‐

Control
Mango Ginger
Exercise
Exercise + Mango Ginger

Glucose, mg/dL
101.57
105.14
91.43
94.29
5.43
0.286

ALT, U/L
70.14
72.71
66.29
75.71
7.03
0.815

AST, U/L
327.14
324.57
325.43
328.14
22.93
0.999

Urea, mg/dL
45.14
45.29
46.14
44.86
2.01
0.973

Creatine, mg/dL
0.96
0.96
0.96
0.96
0.10
0.999

T‐C, mg/dL
75.14a
73.71a
74.00a
68.57b
0.70
0.0001

TG, mg/dL
103.71a
98.43ab
84.29bc
77.71c
4.13
0.002

Lactat, mg/dL
9.66a
9.14a
7.47b
6.61b
0.25
0.0001

ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; T‐C: Tool Cholesterol; TG: Triglycerides; Data are means the standard error of the mean (SEM). Different superscripts (a–c) indicate group mean differences (p < 0.05).

The effects of mango ginger on muscle lipid peroxidation and antioxidant enzymes in exercise‐trained rats

Item
Groups
SEM
‐‐P‐‐

Control
Mango Ginger
Exercise
Exercise + Mango Ginger

MDA, nmol/mg protein
78.84a
71.97a
74.16a
59.49b
2.08
00001

SOD, U/mg protein
0.22c
0.28b
0.34a
0.33ab
0.01
00001

GPx, U/mg protein
143.29b
149.14b
155.71ab
168.43a
4.74
0.008

MDA: Malondialdehyde; SOD: superoxide dismutase; GPx: Glutathione peroxidase. Data are means the standard error of the mean (SEM). Different superscripts (a–c) indicate group mean differences (p < 0.05).

The effects of mango ginger on treadmill running performance in exercise‐trained rats

Item
Groups
SEM
‐‐P‐‐

Control
Mango Ginger
Exercise
Exercise + Mango Ginger

Distance run average per day, m
‐
‐
1037.14
1061.43
8.54
0.126

Run to exhaustion, min
77.31c
76.59c
169.34b
178.73a
3.10
0.0001

Data are means the standard error of the mean (SEM). Different superscripts (a–c) indicate group mean differences (p < 0.05).