bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALDH2 dysfunction and alcohol cooperate in cancer stem cell enrichment
Authors: Flashner Samuel , Shimonosono Masataka , Tomita Yasuto , Matsuura Norihiro , Ohashi Shinya , Muto Manabu , Klein-Szanto Andres J , Alan Diehl J , Chen Che-Hong , Mochly-Rosen Daria , Weinberg Kenneth I , Nakagawa Hiroshi 
Published: November 2023

Abstract
The alcohol metabolite acetaldehyde is a potent human carcinogen linked to esophageal squamous cell carcinoma (ESCC) initiation and development. Aldehyde dehydrogenase 2 (ALDH2) is the primary enzyme that detoxifies acetaldehyde in the mitochondria. Acetaldehyde accumulation causes genotoxic stress in cells expressing the dysfunctional ALDH2E487K dominant negative mutant protein linked to ALDH2*2, the single nucleotide polymorphism highly prevalent among East Asians. Heterozygous ALDH2*2 increases the risk for the development of ESCC and other alcohol-related cancers. Despite its prevalence and link to malignant transformation, how ALDH2 dysfunction influences ESCC pathobiology is incompletely understood. Herein, we characterize how ESCC and preneoplastic cells respond to alcohol exposure using cell lines, three-dimensional organoids and xenograft models. We find that alcohol exposure and ALDH2*2 cooperate to increase putative ESCC cancer stem cells with high CD44 expression (CD44H cells) linked to tumor initiation, repopulation and therapy resistance. Concurrently, ALHD2*2 augmented alcohol-induced reactive oxygen species and DNA damage to promote apoptosis in the non-CD44H cell population. Pharmacological activation of ALDH2 by Alda-1 inhibits this phenotype, suggesting that acetaldehyde is the primary driver of these changes. Additionally, we find that Aldh2 dysfunction affects the response to cisplatin, a chemotherapeutic commonly used for the treatment of ESCC. Aldh2 dysfunction facilitated enrichment of CD44H cells following cisplatin-induced oxidative stress and cell death in murine organoids, highlighting a potential mechanism driving cisplatin resistance. Together, these data provide evidence that ALDH2 dysfunction accelerates ESCC pathogenesis through enrichment of CD44H cells in response to genotoxic stressors such as environmental carcinogens and chemotherapeutic agents.
Defining left behind places: an internationally comparative poset analysis
Authors: Comim Flavio , Abreu Maria , Borges Carolina Guinesi Mattos 
Published: November 2023

AbstractThere is considerable debate in the literature as to the precise definition of ‘left-behind’ places, and the appropriate metrics for identifying them, and for successfully targeting regional policy interventions. We propose an evaluation structure for ‘left-behindness’ based on Amartya Sen’s capability approach, and argue that the diversity of criteria should not be seen as a shortcoming, but rather as a richness to be explored. We show, through the use of ‘posets’ and Hasse diagrams, that there are several distinct ‘structures of left-behindness’ across European NUTS 2 regions, revealing spatial imbalances that do not conform to a ‘one size fits all’ narrative.
The green transition and its potential territorial discontents
Authors: Rodríguez-Pose Andrés , Bartalucci Federico 
Published: November 2023

Abstract
The impacts of climate change are unevenly distributed across territories. Less is known about the potential effects of climate policies aimed at mitigating the negative consequences of climate change while transitioning economies towards low-carbon standards. This paper presents an analytical framework for identifying and assessing the regional impacts of the green transition. We develop a Regional Green Transition Vulnerability Index, a composite measure of the regional vulnerability of European regions to the socio-economic reconfigurations prompted by the green transition. The index brings to light strong regional variations in vulnerability, with less developed, peri-urban and rural regions in Southern and Eastern Europe more exposed to the foreseeable changes brought about by the green transition. We also draw attention to the potential rise of pockets of growing ‘green’ discontent, especially if the green transition contributes, as is likely to be the case, to leaving already left-behind regions further behind.
Assessment of myocardial injuries in ischaemic and non-ischaemic cardiomyopathies using magnetic resonance T1-rho mapping
Authors: Bustin Aurélien , Pineau Xavier , Sridi Soumaya , van Heeswijk Ruud B , Jaïs Pierre , Stuber Matthias , Cochet Hubert 
Published: November 2023

Abstract

Aims
To identify clinical correlates of myocardial T1ρ and to examine how myocardial T1ρ values change under various clinical scenarios.

Methods and results
A total of 66 patients (26% female, median age 57 years [Q1–Q3, 44–65 years]) with known structural heart disease and 44 controls (50% female, median age 47 years [28–57 years]) underwent cardiac magnetic resonance imaging at 1.5 T, including T1ρ mapping, T2 mapping, native T1 mapping, late gadolinium enhancement, and extracellular volume (ECV) imaging. In controls, T1ρ positively related with T2 (P = 0.038) and increased from basal to apical levels (P &lt; 0.001). As compared with controls and remote myocardium, T1ρ significantly increased in all patients’ sub-groups and all types of myocardial injuries: acute and chronic injuries, focal and diffuse tissue abnormalities, as well as ischaemic and non-ischaemic aetiologies (P &lt; 0.05). T1ρ was independently associated with T2 in patients with acute injuries (P = 0.004) and with native T1 and ECV in patients with chronic injuries (P &lt; 0.05). Myocardial T1ρ mapping demonstrated good intra- and inter-observer reproducibility (intraclass correlation coefficient = 0.86 and 0.83, respectively).

Conclusion
Myocardial T1ρ mapping appears to be reproducible and equally sensitive to acute and chronic myocardial injuries, whether of ischaemic or non-ischaemic origins. It may thus be a contrast-agent-free biomarker for gaining new and quantitative insight into myocardial structural disorders. These findings highlight the need for further studies through prospective and randomized trials.
Henry of Lancaster’s Revolt (1328–29): Conflict, the Politics of Kingship, and the Political Public in Fourteenth-Century England
Authors: Raven Matt 
Published: November 2023

Abstract
The late medieval period was an important phase in the history of political communication in England, as more people than ever before became involved in debates about royal governance. The first half of the fourteenth century, however, has been relatively under-studied in this regard. This article analyses a set of arguments put forward during a revolt led by Henry, earl of Lancaster, in 1328–29. After outlining the revolt’s historical and historiographical context, the article reveals how three key themes—the provision of advice; the moral responsibilities attached to royal finance; and the state of the king’s peace—were contested in 1328–29. It then turns to the public orientation of these arguments to suggest that this contest reveals a growing need to engage a political public in order to acquire legitimacy when seeking to reform, or to defend, the direction of royal government. In turn, this helped to set out what the most legitimate paths of political action were and what terms needed to be taken up to describe them by those who wished to involve themselves in the politics of kingship. It is argued that Lancaster’s revolt held a significant position in a much broader history of political communication in England, in which political legitimacy in the exercise of royal authority was claimed before, evaluated by, and generated through a critical body of public opinion which interacted with Plantagenet rule.
CAD/CAM-based 3D-printed and PVS indirect bonding jig system accuracy: a systematic review, meta-analysis, and comparative analysis of hard and soft CAD/CAM transfer trays
Authors: Palone Mario , Fazio Massimo , Pellitteri Federica , Guiducci Daniela , Cremonini Francesca , Pozzetti Ilaria , Tola Martina , Lombardo Luca 
Published: November 2023

Summary

Background
The widespread use of CAD/CAM transfer trays warrants evaluation of their accuracy as compared to PVS transfer trays.

Objectives
To quantify the accuracy of CAD/CAM and PVS transfer trays, investigating any differences between soft and hard trays CAD/CAM transfer trays.

Search methods
Eight different databases (Scopus, Web of Science, PubMed, Google Scholar, ProQuest, Embase, Cochrane Library, ClinicalTrials.gov) were searched, without restrictions, up to an end date of February 2023.

Selection criteria
Clinical trials (randomized and non-randomized) and in vitro studies reporting average imprecision values for bracket positioning obtained by digital superimpositions of digitally planned and real positions.

Data collection and analysis
Data eligibility, data extraction, and risk of bias (RoB-2 and ROBINS-I) were conducted independently. The data, where possible, were synthesized and quantitatively analysed (meta-analysis of mean differences with 95% confidence intervals). The Grade of Recommendation, Assessment, Development and Evaluation (GRADE) analysis of the quality of evidence was performed. The t-test for independent samples was used to compare the transfer accuracy of hard and soft CAD/CAM transfer trays.

Results
Thirteen studies were synthesized in this systematic review, and then eight studies were included in the quantitative meta-analysis. As regards linear measurements, there was a mean transfer error of 0.0752 mm (95%CI: 0.0428, 0.1076) for mesiodistal measures, 0.0943 mm (95%CI: 0.0402, 0.1484) for vertical, and 0.0815 mm (95%CI: 0.0469, 0.1160) for buccolingual. As for angular measurements, there was an average transfer error of 1.2279° (95% CI: 0.6011, 1.8548) for inclination, 0.9397° (95%CI: 0.4672, 1.4123) for angulation, and 0.8721° (95%CI: 0.4257, 1.3185) for rotation. CAD/CAM transfer trays were less accurate than polyvinylsiloxane (PVS) transfer trays, with those made of soft material being more accurate than the hard ones, except for vertical dimension. The GRADE quality of evidence ranged from very low to moderate.

Conclusions and implications
CAD/CAM transfer trays provide high bracket positioning accuracy, with soft transfer trays offering greater precision than rigid ones. Future randomized prospective trials are required to enhance the strength of the available evidence.

Registration
Prospero (CRD42023401278 number)
A response to Joshua Rifkin (‘Singing nuns? More on the story of Verona 761’)
Authors: Stras Laurie 
Published: November 2023

Abstract
A response to Joshua Rifkin’s reconsideration of the early history of Verona, Biblioteca Capitolare, Ms. dcclxi. As well as addressing Rifkin’s objections to my hypotheses, I discuss the motivation behind my scholarship, and some of the choices I make in presenting it.
Use of secondary prevention medications in metropolitan and non-metropolitan areas: an analysis of 41 925 myocardial infarctions in Australia
Authors: Livori Adam C , Ademi Zanfina , Ilomäki Jenni , Pol Derk , Morton Jedidiah I , Bell J Simon 
Published: November 2023

Abstract

Aims
People in remote areas may have more difficulty accessing healthcare following myocardial infarction (MI) than people in metropolitan areas. We determined whether remoteness was associated with initial and 12-month use of secondary prevention medications following MI in Victoria, Australia.

Methods and results
We included all people alive at least 90 days after discharge following MI between July 2012 and June 2017 in Victoria, Australia (n = 41 925). We investigated dispensing of P2Y12 inhibitors (P2Y12i), statins, angiotensin-converting enzyme inhibitors or angiotensin receptor blockers (ACEIs/ARBs), and beta-blockers within 90 days after discharge. We estimated 12-month medication use using proportion of days covered (PDC). Remoteness was determined using the Accessibility/Remoteness Index of Australia (ARIA). Data were analysed using adjusted parametric regression models stratified by ST elevation myocardial infarction (STEMI) and non-STEMI (NSTEMI). There were 10 819 STEMI admissions and 31 106 NSTEMI admissions. Following adjustment across NSTEMI and STEMI, there were no medication classes dispensed in the 90-day post-discharge that differed in a clinically significant way from the least remote (ARIA = 0) to the most remote (ARIA = 4.8) areas. The largest difference for NSTEMI was ACEI/ARB, with 71% (95% confidence interval 70–72%) vs. 80% (76–83%). For STEMI, it was statins with 89% (88–90%) vs. 95% (91–97%). Predicted PDC for STEMI and NSTEMI was not clinically significant across remoteness, with the largest difference in NSTEMI being P2Y12i with 48% (47–50%) vs. 55% (51–59%), and in STEMI, it was ACEI/ARB with 68% (67–69%) vs. 76% (70–80%).

Conclusion
Remoteness does not appear to be a clinically significant driver for medication use following MI. Possible differences in cardiovascular outcomes in metropolitan and non-metropolitan areas are not likely to be explained by access to secondary prevention medications.
Variation in follow-up for children born very preterm in Europe
Authors: Seppänen Anna-Veera , Barros Henrique , Draper Elizabeth S , Petrou Stavros , Andronis Lazaros , Kim Sungwook , Maier Rolf F , Pedersen Pernille , Gadzinowski Janusz , Pierrat Véronique , Sarrechia Iemke , Lebeer Jo , Ådén Ulrika , Toome Liis , Thiele Nicole , van Heijst Arno , Cuttini Marina , Zeitlin Jennifer ,   The SHIPS Research Group, Lebeer J , Sarrechia I , Van Reempts P , Bruneel E , Cloet E , Oostra A , Ortibus E , Boerch K , Pedersen P , Toome L , Varendi H , Männamaa M , Ancel P Y , Burguet A , Jarreau P H , Pierrat V , Nuytten A , Maier R F , Zemlin M , Misselwitz B , Wohlers L , Cuttini M , Croci I , Carnielli V , Ancora G , Faldella G , Ferrari F , van Heijst A , Koopman-Esseboom C , Gadzinowski J , Mazela J , Montgomery A , Pikuła T , Barros H , Costa R , Rodrigues C , Aden U , Draper E S , Fenton A , Johnson S J , Mader S , Thiele N , Petrou S , Kim S W , Andronis L , Zeitlin J , Aubert A M , Bonnet C , El Rafei R , Seppanen A V 
Published: November 2023

Abstract

Background
Children born very preterm (&lt;32 weeks of gestation) face high risks of neurodevelopmental and health difficulties compared with children born at term. Follow-up after discharge from the neonatal intensive care unit is essential to ensure early detection and intervention, but data on policy approaches are sparse.

Methods
We investigated the characteristics of follow-up policy and programmes in 11 European countries from 2011 to 2022 using healthcare informant questionnaires and the published/grey literature. We further explored how one aspect of follow-up, its recommended duration, may be reflected in the percent of parents reporting that their children are receiving follow-up services at 5 years of age in these countries using data from an area-based cohort of very preterm births in 2011/12 (N = 3635).

Results
Between 2011/12 and 22, the number of countries with follow-up policies or programmes increased from 6 to 11. The policies and programmes were heterogeneous in eligibility criteria, duration and content. In countries that recommended longer follow-up, parent-reported follow-up rates at 5 years of age were higher, especially among the highest risk children, born &lt;28 weeks’ gestation or with birthweight &lt;1000 g: between 42.1% and 70.1%, vs. &lt;20% in most countries without recommendations.

Conclusions
Large variations exist in follow-up policies and programmes for children born very preterm in Europe; differences in recommended duration translate into cross-country disparities in reported follow-up at 5 years of age.
Soil-specific responses in the antibiotic resistome of culturable Acinetobacter spp. and other non-fermentative Gram-negative bacteria following experimental manure application
Authors: Sardar Puspendu , Elhottová Dana , Pérez-Valera Eduardo 
Published: November 2023

Abstract
Acinetobacter spp. and other non-fermenting Gram-negative bacteria (NFGNB) represent an important group of opportunistic pathogens due to their propensity for multiple, intrinsic, or acquired antimicrobial resistance (AMR). Antimicrobial resistant bacteria and their genes can spread to the environment through livestock manure. This study investigated the effects of fresh manure from dairy cows under antibiotic prophylaxis on the antibiotic resistome and AMR hosts in microcosms using pasture soil. We specifically focused on culturable Acinetobacter spp. and other NFGNB using CHROMagar Acinetobacter. We conducted two 28-days incubation experiments to simulate natural deposition of fresh manure on pasture soil and evaluated the effects on antibiotic resistance genes (ARGs) and bacterial hosts through shotgun metagenomics. We found that manure application altered the abundance and composition of ARGs and their bacterial hosts, and that the effects depended on the soil source. Manure enriched the antibiotic resistome of bacteria only in the soil where native bacteria had a low abundance of ARGs. Our study highlights the role of native soil bacteria in modulating the consequences of manure deposition on soil and confirms the potential of culturable Acinetobacter spp. and other NFGNB to accumulate AMR in pasture soil receiving fresh manure.
Fifty-years of theory-driven research in HCR: prominence, progress, and opportunities
Authors: Shue Carolyn Karmon , O’Hara Laura L S , Stamp Glen 
Published: November 2023

Abstract
We reviewed Human Communication Research (HCR) articles that included named theories in the: (a) abstract, (b) keywords, or (c) section headers to determine theory trends, identifying 592 instances of named theories in 447 articles. We conducted a follow-up analysis of 76 articles to illustrate how HCR researchers have contributed to theory development. Our review demonstrated systematic growth in theory use; the number of named theories in our sample doubled from the first 10 years to the last. There remains the propensity for theory shopping in the discipline with over 50% of theories in each decade cited only once. We also analyzed theory development strategies. There was evidence of theorizing that: (a) extended the range of theories and (b) explored effects. There was less theory synthesis that might provide frameworks for organizing related but distinct theories. We concluded by offering recommendations for how researchers can contribute to future theoretical development.
Coordinating external assistance for UHC: Pakistan’s early experience of the SDG3 GAP
Authors: Khalid Faraz , Afzal Sabeen , Shirazi Ali , Quick Isadora , Mataria Awad 
Published: November 2023

AbstractPakistan is a lower middle-income setting. External assistance for health and development partners play an important role in health sector development. The federal system and health care delivery as a devolved provincial subject warrant regular and effective coordination among federating units and partners. Pakistan was selected as a priority country in 2019 for the implementation of the Global Action Plan for Healthy Lives and Well-being for All (SDG3 GAP). Given the ongoing universal health coverage (UHC) reforms, the country prioritized two SDG3 GAP accelerators, related to primary health care (PHC) and sustainable financing for health (SFH). Eight partner agencies representing PHC and SFH accelerators jointly planned and conducted a ‘PHC for UHC mission’ to Pakistan in 2021. This mission paved the way for setting up an SDG3 GAP Coordination Committee and a ‘PHC Service Delivery and Financing working group’, which have been regularly coming together through in-person and virtual meetings; the latter was relatively uncommon previously and this new way of working provided a chance to build rapport, share workplans, identify duplications in technical assistance and jointly gauge governments’ priorities. This has shifted the focus of reforms deliberations from ‘what’ to ‘how’, enabling joint strategic planning and implementation. The joint work by SDG3 GAP partners with the Ministry of National Health Services Regulation & Coordination linked discussions on health financing and service delivery reforms for the first time, and has contributed to advocacy, analysis, strategic policy dialogue, institutional strengthening and implementation of UHC reforms, with a focus on PHC. Even though joint work by SDG3 GAP partners undoubtedly shows the potential for better alignment and collaboration, translation of the commitment to better collaboration into concrete impact has been the result of committed and engaged staff members, rather than institutionalized change, which will require strong incentives for collaboration.
Gap-free genome assembly andCYP450gene family analysis reveal the biosynthesis of anthocyanins inScutellaria baicalensis
Authors: Pei Tianlin , Zhu Sanming , Liao Weizhi , Fang Yumin , Liu Jie , Kong Yu , Yan Mengxiao , Cui Mengying , Zhao Qing 
Published: November 2023

AbstractScutellaria baicalensis Georgi, a member of the Lamiaceae family, is a widely utilized medicinal plant. The flavones extracted from S. baicalensis contribute to numerous health benefits, including anti-inflammatory, antiviral, and anti-tumor activities. However, the incomplete genome assembly hinders biological studies on S. baicalensis. This study presents the first telomere-to-telomere (T2T) gap-free genome assembly of S. baicalensis through the integration of Pacbio HiFi, Nanopore ultra-long and Hi-C technologies. A total of 384.59 Mb of genome size with a contig N50 of 42.44 Mb was obtained, and all sequences were anchored into nine pseudochromosomes without any gap or mismatch. In addition, we analysed the major cyanidin- and delphinidin-based anthocyanins involved in the determination of blue-purple flower using a widely-targeted metabolome approach. Based on the genome-wide identification of Cytochrome P450 (CYP450) gene family, three genes (SbFBH1, 2, and 5) encoding flavonoid 3′-hydroxylases (F3′Hs) and one gene (SbFBH7) encoding flavonoid 3′5′-hydroxylase (F3′5′H) were found to hydroxylate the B-ring of flavonoids. Our studies enrich the genomic information available for the Lamiaceae family and provide a toolkit for discovering CYP450 genes involved in the flavonoid decoration.