bluuebunny/crossref_metadata_2025_split huggingface dataset:
MIR4435-2HG: A Novel Biomarker for Triple-Negative Breast Cancer Diagnosis and Prognosis, Driving Tumor Progression through EMT by JNK/c-Jun and p38 MAPK Signaling Pathway Activation
Authors: Gu Peng , Ding Wentao , Zhu Wenting , Shen Ling , Yan Bin , Zhang Lei , Wang Wei , Wang Ruitao , Wang Wenhao , Wang Yanhao , Sun Xing 
Published: January 2024

Abstract
Background
Breast cancer has the highest incidence rate and causes the most fatalities among all female cancers worldwide. Triple-negative breast cancer (TNBC) is known for its strong invasiveness and higher rates of recurrence. In this research, we aimed to identify MIR4435-2HG as a promising long non-coding RNA (lncRNA) biomarker and therapeutic target for TNBC.
Methods
Utilizing clinicopathological information and transcriptome data from The Cancer Genome Atlas (TCGA) database, we assessed the clinical relevance of MIR4435-2HG in breast cancer through univariate and multivariate COX regression, receiver operating characteristic (ROC) analysis, as well as Kaplan-Meier survival analysis. To investigate the biological role of MIR4435-2HG in TNBC, we conducted gene set enrichment analysis (GSEA), as well as Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Additionally, we constructed and validated a nomogram to predict disease-free survival (DFS). Both the R package “pRRophetic” and the Tumor Immune Dysfunction and Exclusion (TIDE) algorithm were employed to forecast the sensitivity to different therapeutics between the high- and low-MIR4435-2HG groups. We employed single-cell RNA sequencing analysis and tumor microenvironment infiltration analysis to investigate the potential involvement of MIR4435-2HG in the TNBC tumor microenvironment. Cellular biological behaviors were assessed utilizing CCK-8, transwell assays, and wound-healing assays. Furthermore, we performed RNA-seq, qRT-PCR, and western blotting analyses to elucidate and confirm the specific mechanisms underlying MIR4435-2HG-mediated TNBC progression.
Results
In our study, we have identified MIR4435-2HG as a significant diagnostic and prognostic factor for TNBC. We observed that MIR4435-2HG is widely expressed and might have a significant impact on the reshaping of the TNBC tumor microenvironment. Patients with breast cancer in the high-MIR4435-2HG group may show reduced sensitivity to cisplatin, doxorubicin, and gemcitabine and have an increased propensity for immune escape. Notably, MIR4435-2HG predominantly enhances the migratory and invasive capabilities of TNBC cells through the epithelial-mesenchymal transition (EMT) process. Mechanistically, we validated that MIR4435-2HG activates the JNK/c-Jun and p38 MAPK signaling pathway in TNBC.
Conclusions
Our findings highlight the significant potential of MIR4435-2HG as a highly promising biomarker for TNBC. Targeting MIR4435-2HG could represent an appealing therapeutic approach to suppress TNBC metastasis.
Pediatric acute myeloid leukemia with t(8;21) and KIT mutation treatment with avapritinib post-stem cell transplantation: a case report
Authors: Wang Qingwei , Hu Yixin , Gao Li , Zhang Senlin , Lu Jun , Li Bohan , Li Jie , Yao Yanhua , Cheng Shengqin , Xiao Peifang , Hu Shaoyan 
Published: January 2024

Abstract
Acute myeloid leukemia (AML) with t(8;21) (q22;q22), which forms RUNX1::RUNX1T1 fusion gene, is classified as a favorable-risk group. However, the presence of mutations in KITexon 17 results in an adverse prognosis in this group. Avapritinib, a novel tyrosine kinase inhibitor, was designed to target KIT mutation. We report a retrospective study of four pediatric patients with AML with t(8:21) and KIT exon 17 mutation who were treated with avapritinib. Three of these patients failed to demethylate drugs and donor lymphocyte infusion targeting RUNX1::RUNX1T1-positivity after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Three patients with RUNX1::RUNX1T1positivity had turned negative after 1, 2, and 7 months of avapritinib treatment and remained so during follow-up, while one patient retained the lowest relative value for months. The common adverse effect of avapritinib is neutropenia, which is well-tolerated. This case series indicates that avapritinib may be effective and safe for preemptive treatment of children with AML with t(8;21) and KIT mutation after allo-HSCT, providing a treatment option for preventing relapse after allo-HSCT.
Homoclinic bifurcations and chaotic dynamics in a bistable vibro-impact SD oscillator under Gaussian white noise
Authors: Jia Lele , Li Shuangbao , Kou Liying , Wu Kongran 
Published: January 2024

Abstract
This paper studies the effect of Gaussian white noise on homoclinic bifurcations and chaotic dynamics of a bistable vibro-impact SD oscillator. Firstly, the SD oscillator is reproduced and generalized by installing a slider on a fixed rod so that the slider is connected by a pair of linear springs initially pre-compressed in the vertical direction to achieve bistble vibration characteristic, two screw nuts are installed on the rod as two adjustable bilateral rigid constraints to generate vibro-impact. A discontinuous dynamical equation with a map defined on switching boundaries to represent velocity loss during each collision is derived to describe the vibration pattern of the bistable vibro-impact SD oscillator through studying the persistence of the unique unperturbed non-smooth homoclinic structure. Secondly, the general framework of random Melnikov process for a class of bistable vibro-impact systems under Gaussian white noise is simply derived and employed through the corresponding Melnikov function to obtain the necessary parameter thresholds for homoclinic tangency and possible chaos of the bistable vibro-impact SD oscillator. Thirdly, the effectiveness of a semi-analytical prediction by the Melnikov function is verified through the lagest Lyapunov exponent, bifurcation series, and 0 − 1 test. In addition, the sensitivity to the initial values of chaos is verified by the fractal of attractor basins, and the influence of the Gaussian white noise on periodic and chaotic structures is studied through Poincaré mapping to show rich dynamical geometric structures.
Variable Effects of Experimental Sea-Level Rise Conditions and Invasive Species on California cordgrass
Authors: Gonzalez Julie , Ferner Matthew C. , Grosholz Edwin D. 
Published: January 2024

Abstract

Sea-level rise (SLR) will produce unprecedented changes in tidal marsh systems that already cope with daily tidal perturbations, disturbances from storms, and salinity changes from droughts and runoff events. Additionally, negative impacts from non-native invasive species may alter marsh plants’ susceptibility to SLR stressors like inundation and salinity. Increasingly, tidal marsh communities must tolerate both changes in the physical environment from SLR and invasive species impacts. To assess the response of a threatened tidal marsh cordgrass (Spartina foliosa) to both stressors, we implemented a field experiment in San Francisco Bay, CA, USA, exposing cordgrass to a treatment that extended tidal inundation projected with SLR using a recently developed in situ method. At one of two field sites, we also enclosed the cordgrass with or without the invasive European green crab, Carcinus maenas. We found that cordgrass responded negatively to longer inundation, although these effects varied by site and year. In higher inundation treatments, cordgrass survival increased with increasing surface elevation of the plot. Cordgrass survival was lower in the presence of invasive crabs relative to controls. We did not find interacting effects of increased inundation and green crab presence on any response variables, which highlights the need to consider how latent or sequential effects of multiple stressors may affect ecosystems. This study demonstrates significant biological responses to invasive species and inundation. Evaluating relative effects and timing of multiple stressors, especially those induced by climate change and invasive species, will help us to manage threatened ecological communities in a changing world.
VBP1 promotes tumor proliferation as a part of the hypoxia-related signature in esophageal squamous cell carcinoma
Authors: Miao Huikai , Gao Wuyou , Zhong Leqi , Li Hongmu , Chen Dongni , Xu Chunmei , Wen Zhesheng , Chen Youfang 
Published: January 2024

Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor in East Asia. Hypoxia, a hallmark of solid tumors, significantly alters redox homeostasis inside tumor microenvironment. This alteration drives tumor proliferation, invasion, and metastasis, leading to poor prognostic outcomes. However, the role of hypoxia-related genes in ESCC remains poorly understood. We employed RNA sequencing to identify differentially expressed genes in ESCC. Clinical data, transcriptome profiles, and a hypoxia-related gene set were extracted from open-source databases. A prognostic model was constructed using least absolute shrinkage and selection operator (LASSO) regression, which was then validated through Cox regression analysis. Within this prognostic model, we pinpointed and investigated a key hypoxia-related gene affecting prognosis. The gene's expression was validated using real-time PCR and immunohistochemistry in both esophageal carcinoma and normal tissues. Tumor proliferation was examined through in vitro and in vivo assays, including the Cell Counting Kit-8, EdU, colony formation, and subcutaneous tumor models. A robust four-gene prognostic model (VBP1, BGN, CDKN1A, and PPFIA1) was successfully constructed and validated. Among these, VBP1 emerged as a key gene, exhibiting high expression levels that correlated with poor prognosis in ESCC. Functional experiments confirmed that VBP1 significantly accelerated tumor proliferation both in vitro and in vivo. VBP1 is identified as a pivotal gene within the hypoxia-related prognostic signature, and it significantly promotes tumor proliferation in ESCC.
The effect of social alienation on stigma among in-patients with stroke: A cross-sectional study
Authors: Wu Yu , Liu Jian , Fornah Lovel , Zhao Jun , Wu Shicai 
Published: January 2024

Abstract
Backgroud Stigma adversely affects the rehabilitation among in-patients with stroke, which decreases the motivation and functional improvement during rehabilitation in patients with stroke. The study aims to investigate the influencing factors of stigma. Methods The study design is a cross-sectional survey. A total of 220 in-patients with stroke completed demographic information, the generalized social alienation scale, and the stroke stigma scale. Multiple linear regression analysis was used to evaluate the impact of the diverse factors on stigma. Results The findings indicate that there were moderate scores on stigma among in-patients with stroke. Age(B=−2.105, P < 0.001), level of education[Junior high school(B = 1.545, P = 0.040), senior high school or technical secondary school(B = 3.480, P < 0.001), Undergraduate or junior college(B = 3.716, P < 0.001), and graduate(B = 5.483, P < 0.001)] and social alienation(B = 0.158, P < 0.001) were all significantly associated with stigma in patients with stroke. Conclusions To address stigma among in-patients with stroke, efforts should be directed towards reducing social alienation among younger patients and those with higher levels of education.
WITHDRAWN: Cross-scale modeling of Microencapsulated self-healing composite with multiphase medium and their damage competition behavior
Authors: Yin Haipeng , Li Youtang , Huang Hua 
Published: January 2024

Abstract
Constructing a high-fidelity cross-scale numerical model is the primary challenge in the multiscale analysis of multiphase medium composites. Considering the limitations of the Finite Element Method (FEM) in the study of the dynamic damage problem of non-homogeneous and discontinuous materials, the Discrete Element Method (DEM) is used in this study to build a three-medium multiscale numerical model containing aggregates, matrix, and microcapsules. Further, the dynamic damage problem of microcapsules embedded in self-healing composites and the damage competition issues between the microcapsules and the matrix are investigated. The results indicate that (1) The damage evolution process, the location of maximum damage, and the final damage pattern of microcapsule embedded in the matrix and exposed to the ideal environment differed considerably. (2) The effect of microcapsule volume fraction on the mechanical strength of the matrix in self-healing composites is not linearly negatively correlated, but instead, there is a non-linear relationship, which is highly dependent on the strength ratio of the microcapsule to the matrix. (3) In self-healing composites, damage competition between the microcapsules and the matrix has a decisive influence on triggering the self-healing mechanism. (4) To obtain a better self-healing effect, the microcapsule volume fraction in the self-healing composites should not exceed 1.5%, and the mechanical strength pre-maintenance should not be more than 95% of the failure strength. The multiphase medium & multiscale analysis method proposed in this study also provides a new approach for visualizing the progressive dynamic damage problem in self-healing composites.
Energy Confinement of Mode-Localised Sensors
Authors: Chang Honglong , Zhang Zhao , Li Han , Hou Cheng , Hao Yongcun 
Published: January 2024

Abstract

Energy confinement is one of the significant features of the mode-localised phenomenon and is also the secret behind the high sensitivity of mode-localised sensors. Surprisingly, limited attention has been directed towards comprehensively elucidating the phenomenon of energy confinement within a specific resonator in weakly coupled resonators through detailed physical analysis. In this paper, a novel concept of modal dominance is proposed for the first time to elucidate the energy confinement of mode-localised sensors. The mode frequencies of the weakly coupled resonant system are dominated by the natural frequencies of the resonators in the system and the relationship between the two frequencies determines the resonant states, and then the amplitudes of the resonators. When perturbations break the system symmetry, the resonator in resonance acquires the larger amplitude while the amplitude of the out-of resonance resonator decreases. The concept of modal dominance is illustrated comprehensively and experimentally proved.
Age-related loss of intestinal barrier integrity plays an integral role in Thymic involution and T cell ageing
Authors: Conway Jessica , DeJong Erica N , White Andrea Andrea J , Dugan Ben , Rees Nia Paddison , Parnell Sonia M , Lamberte Lisa , Sharma-Oates Archana , Sullivan Jack , Mauro Claudio , Schaik Willem van , Anderson Graham , Bowdish Dawn M E , Duggal Niharika A 
Published: January 2024

Abstract

The intestinal epithelium serves as a physical and functional barrier against harmful substances, preventing their entry into the circulation and subsequent induction of a systemic immune response. Gut barrier dysfunction has recently emerged as a feature of ageing linked to declining health, and increased intestinal membrane permeability has been shown to promote heightened systemic inflammation in aged hosts. Concurrent with age-related changes in the gut microbiome, the thymic microenvironment undergoes a series of morphological, phenotypical and architectural alterations with age, including disorganisation of the corticomedullary junction, increased fibrosis, increased thymic adiposity and the accumulation of senescent cells. However, a direct link between gut barrier dysbiosis and thymic involution leading to features of immune ageing has not been explored thus far. Herein, we identify several strong associations between enhanced microbial translocation and the peripheral accumulation of terminally differentiated, senescent and exhausted T cells and the compensatory expansion of regulatory T cells in older adults. Most importantly, we confirm a direct effect of mucosal permeability on the regulation of thymic ageing and hyperactivation of the immune system by demonstrating that aged germ-free mice are protected from age-related intestinal membrane permeability. Together, these findings establish a mechanism by which gut barrier dysfunction drives systemic activation of the immune system during ageing, via causing thymic involution, extending our understanding of the consequences of intestinal membrane permeability and opening up the possibility for the use of microbiome-based interventions to restore immune homeostasis in older adults.
Self-injury functions mediate the association between anxiety and self-injury frequency among depressed Chinese adolescents: Gender differences
Authors: Zhao Yunhan , Zhao Xudong , Zhou Yongjie , Liu Liang 
Published: January 2024

Abstract
Background
Non-suicidal self-injury (NSSI) has become a common clinical problem that severely threatens the mental and physical health of Chinese adolescents. This study explores the mediation effects of NSSI functions on the relationship between anxiety and NSSI frequency among depressed Chinese adolescents as well as the gender differences in the mediating effects.
Methods
In this study, a cross-sectional survey method was used to obtain data of 1773 adolescent patients with major depression disorders from over 20 specialized psychiatric hospitals across multiple provinces in China. A self-designed questionnaire for demographic information, the NSSI function assessment scale, and the generalized anxiety disorder scale were employed to investigate demographic data, NSSI frequency, NSSI functions, and anxiety and to analyze the mediating effects of NSSI functions on the association between anxiety and NSSI frequency among adolescents of different genders.
Results
A total of 316 male patients and 1457 female patients were investigated. Female patients had a higher NSSI frequency (Z = 3.195, P = 0.001) and higher anxiety scores than did male patients (Z = 2.714, P = 0.007). Anxiety had a stronger positive predictive effect on the NSSI frequency in females (OR = 1.090) than in males (OR = 1.064). For male patients, the emotion regulation function in NSSI motivation played a full mediating role in the association between anxiety and NSSI frequency. For female patients, the emotion regulation and social avoidance functions in NSSI functions played a partial mediating role between anxiety and NSSI frequency.
Conclusions
There are gender differences in the mediating role of NSSI functions of depressed adolescents in the association between anxiety and NSSI frequency. When experiencing anxiety, males may engage in NSSI behaviors as a means to regulate their emotions. For females, anxiety can directly predict NSSI frequency, and they may attempt NSSI to alleviate part of their anxiety and achieve the purpose of rejecting others. In the face of anxiety among depressed adolescents of different genders, developing different emotional regulation methods and behavioral regulation strategies may be critical in preventing their NSSI behaviors.
MicroRNAs Associated with Metformin Treatment in the Diabetes Prevention Program
Authors: Lewis Kimberly A. , Stroebel Benjamin , Zhang Li , Aouizerat Bradley , Mattis Aras , Flowers Elena 
Published: January 2024

Abstract

The Diabetes Prevention Program (DPP) randomized controlled trial demonstrated that metformin treatment reduced progression to type 2 diabetes (T2D) by 31% compared to placebo in adults with prediabetes. Circulating micro-ribonucleic acids (miRs) are promising biomarkers of T2D risk, but little is known about their associations with metformin regimens for T2D risk reduction. We compared the change in 24 circulating miRs from baseline to 2 years in a subset from DPP metformin intervention (n = 50) and placebo (n = 50) groups using Wilcoxon signed rank tests. Spearman’s correlations were used to evaluate associations between miR change and baseline clinical characteristics. Multiple linear regression was used to adjust for covariates. The sample was 73% female, 17% Black, 13% Hispanic, and 50 ± 11 years. Participants were obese, normotensive, prediabetic, and dyslipidemic. Change in 12 miR levels from baseline to 2 years was significantly different in the metformin group compared with placebo after adjusting for multiple comparisons: six (let-7c-5p, miR-151a-3p, miR-17-5p, miR-20b-5p, miR-29b-3p, and miR-93-5p) were significantly upregulated and six (miR-130b-3p, miR-22-3p, miR-222-3p, miR-320a-3p, miR-320c, miR-92a-3p) were significantly downregulated in the metformin group. These miRs help to explain how metformin is linked to T2D risk reduction, which may lead to novel biomarkers, therapeutics, and precision-health strategies.
Research on WSN coverage optimization of improved dung beetle algorithm in 3D environment
Authors: Xiaodong Wang , yang yijie , Yuqi LV , Chang LIU , Xuanzhong Chen , Bo Xie , Qingwen DU 
Published: January 2024

Abstract
Aiming at the difficulties in node deployment optimization of wireless sensor networks and the establishment of low-energy wireless sensor networks, this paper proposes an optimization scheme based on improved dung beetle optimization algorithm. Firstly, random sine-cosine perturbation strategy and uniform distribution dynamic reverse strategy are adopted to expand the algorithm search space, and then nonlinear and random perturbation strategy is adopted to improve the convergence factor. Finally, the local search strategy of Sparrow search algorithm is adopted to enhance the development ability of the algorithm. The benchmark function test of the algorithm and the optimization of wireless sensor node coverage are carried out. The results show that the algorithm has a good convergence speed and accuracy compared with other excellent algorithms, effectively improves the network coverage, and the optimized node distribution is more uniform.
How Were European GPS/FPS Involved in the Covid Vaccination-Campaign? A European Questionnaire Study About the Experiences of the Vaccinations in 2021
Authors: Rurik Imre , Torzsa Péter 
Published: January 2024

Abstract
Background. The SARS-CoV-2pandemic has become the greatest public health challenge worldwide. Intensive research started to develop vaccines and by the late 2020 vaccination campaign started.
Aim. Collecting information from European countries, how and in what extent were family physicians/general practitioners/FPs/GPs involved in the vaccinations-campaign in 2021 and how was it organized at national level.
Method. A short questionnaire was distributed through the secretariats of WONCA-Europe and the European Forum for Primary Care.
Results. In most of the countries participation of FPs/GPs was compulsory. The vaccination was usually centrally organized by governmental authorities. At the beginning (web-based) registration of patients was required, mainly at national level. By the mid of 2021, vaccination on walk in basis became available in almost every country for the first and for the booster injections. The remunerations of GPs/FPs were differed, in some countries without extra payment. The Pfizer’s vaccine was used in all countries; while in 9 countries not EMA approved vaccines were also used in primary care settings and at vaccination centres. In some countries, professional homepages helped the GPs.
The involvement of PHC providers did not correlate to the vaccination coverage of the entire population of the respective countries. It was the highest in the more developed countries with higher living standard, where participation of GPs was voluntary and appropriate financial incentives were offered for them.
Conclusion. The vaccination campaign was a professional and logistic challenge and an excellent performance of PC providers. Experiences gained could be used in the future to manage similar pandemic challenges.