bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soft and hard tissue response to photocrosslinked poly(propylene fumarate) scaffolds in a rabbit model
Authors: Fisher John P. , Vehof Johan W. M. , Dean David , van der Waerden Jan Paul C. M. , Holland Theresa A. , Mikos Antonios G. , Jansen John A. 
Published: August 2002

AbstractThe treatment of large cranial defects may be greatly improved by the development of precisely formed bone tissue engineering scaffolds. Such scaffolds could be constructed by using UV laser stereolithography to photocrosslink a linear, biodegradable polymer into a three‐dimensional implant. We have previously presented a method to photocrosslink the biodegradable polyester, poly(propylene fumarate) (PPF). To ensure the safety and effectiveness of this technique, the soft and hard tissue response to photocrosslinked PPF scaffolds of different pore morphologies was investigated. Four classes of photocrosslinked PPF scaffolds, constructed with differing porosities (57–75%) and pore sizes (300–500 or 600–800 μm), were implanted both subcutaneously and in 6.3‐mm‐diameter cranial defects in a rabbit model. The rabbits were sacrificed at 2 and 8 weeks, and the implants were analyzed by light microscopy, histological scoring analysis, and histomorphometric analysis. Results showed the PPF scaffolds elicit a mild tissue response in both soft and hard tissues. Inflammatory cells, vascularization, and connective tissue were observed at 2 weeks; a decrease in inflammatory cell density and a more organized connective tissue were observed at 8 weeks. Scaffold porosity and scaffold pore size were not found to significantly affect the observed tissue response. Evidence of scaffold surface degradation was noted both by histology and histomorphometric analysis. Bone ingrowth in PPF scaffolds implanted into cranial defects was <3% of the defect area. The results indicate that photocrosslinked PPF scaffolds are biocompatible in both soft and hard tissues and thus may be an attractive platform for bone tissue engineering. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 547–556, 2002
Displacement of histamine from liver cells and cell components by ligands for cytochromes P450
Authors: Brandes Lorne J. , Queen Gary M. , LaBella Frank S. 
Published: August 2002

AbstractIntracellular histamine (HA) and cytochrome P450 monooxygenases (P450) each have been proposed as mediators of cell function, growth, and proliferation. The P450 family of heme enzymes is found in virtually all cells and generates, transforms, or inactivates steroids and other lipids that participate in cell regulation. We previously demonstrated a second messenger role for HA in blood platelets and the formation of a HA‐P450 heme complex when exogenous HA was added to microsomes isolated from rat liver cells or to purified human P450 isozymes. Employing a radioimmunoassay, we now demonstrate that rat liver slices, microsomes derived from the livers of adult male rats and mast cell‐deficient mice, and hepatoma cells, all contain endogenous HA. HA release from microsomes into the incubation medium, as determined by radioimmunoassay, is enhanced in the presence of carbon monoxide, steroids, and certain drugs, all agents that unite either directly with the iron atom or bind elsewhere within the heme cavity. Rat liver slices preincubated with 3H‐HA release labeled amine into the medium in the presence of those same ligands. These findings provide evidence of an in situ HA‐P450 complex and offer further support that the imidazole, HA, is a physiological, intracellular modulator of cytochromes P450 in liver cells, and perhaps of these and other heme proteins in tissues in general. J. Cell. Biochem. 85: 820–824, 2002. © 2002 Wiley‐Liss, Inc.
p53 down‐regulates human bradykinin B1 receptor gene expression
Authors: Yang Xionghu , Taylor Linda , Polgar Peter 
Published: August 2002

AbstractThe tumor suppressor, p53, has been shown to transcriptionally activate or silence a number of target genes. As an activator, p53 relies on its specific consensus sequence within the promoter. It is not clear whether p53 requires a specific DNA binding site in its action as a gene repressor. This report demonstrates that the human BKB1R gene is a p53 target. Expression of p53 in transiently transfected SV40‐transformed IMR90 cells strongly suppressed luciferase reporter activity driven by a 1.8 kb BKB1R promoter as well as its minigene. These down‐regulations were p53 dose‐dependent. p53 reduced both basal and induced promoter activities of the minigene. Expression of p53 abolished the inducibility of the minigene. Induction of endogenous p53 expression by etoposide also inhibited promoter activity and minigene inducibility. Replacing the region containing both the putative p53 binding site and the TATA‐box with a basal adenovirus promoter in the 1.8 kb promoter construct did not prevent p53 from inhibiting BKB1R promoter activity. Thus suppression by p53 is not mediated by competition with the TATA‐binding protein and is not through interaction with the putative p53‐binding site. p53 also does not appear to suppress BKB1R gene expression through interaction with c‐Jun which functions in the inducibility of this gene [Yang et al., 2001]. J. Cell. Biochem. 82: 38–45, 2001. © 2001 Wiley‐Liss, Inc.
Chemistry with ADF
Authors: te Velde G. , Bickelhaupt F. M. , Baerends E. J. , Fonseca Guerra C. , van Gisbergen S. J. A. , Snijders J. G. , Ziegler T. 
Published: August 2002

AbstractWe present the theoretical and technical foundations of the Amsterdam Density Functional (ADF) program with a survey of the characteristics of the code (numerical integration, density fitting for the Coulomb potential, and STO basis functions). Recent developments enhance the efficiency of ADF (e.g., parallelization, near order‐N scaling, QM/MM) and its functionality (e.g., NMR chemical shifts, COSMO solvent effects, ZORA relativistic method, excitation energies, frequency‐dependent (hyper)polarizabilities, atomic VDD charges). In the Applications section we discuss the physical model of the electronic structure and the chemical bond, i.e., the Kohn–Sham molecular orbital (MO) theory, and illustrate the power of the Kohn–Sham MO model in conjunction with the ADF‐typical fragment approach to quantitatively understand and predict chemical phenomena. We review the “Activation‐strain TS interaction” (ATS) model of chemical reactivity as a conceptual framework for understanding how activation barriers of various types of (competing) reaction mechanisms arise and how they may be controlled, for example, in organic chemistry or homogeneous catalysis. Finally, we include a brief discussion of exemplary applications in the field of biochemistry (structure and bonding of DNA) and of time‐dependent density functional theory (TDDFT) to indicate how this development further reinforces the ADF tools for the analysis of chemical phenomena. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 931–967, 2001
6‐31G* basis set for third‐row atoms
Authors: Rassolov Vitaly A. , Ratner Mark A. , Pople John A. , Redfern Paul C. , Curtiss Larry A. 
Published: August 2002

AbstractMedium basis sets based upon contractions of Gaussian primitives are developed for the third‐row elements Ga through Kr. The basis functions generalize the 6‐31G and 6‐31G* sets commonly used for atoms up to Ar. A reexamination of the 6‐31G* basis set for K and Ca developed earlier leads to the inclusion of 3d orbitals into the valence space for these atoms. Now the 6‐31G basis for the whole third‐row K through Kr has six primitive Gaussians for 1s, 2s, 2p, 3s, and 3p orbitals, and a split‐valence pair of three and one primitives for valence orbitals, which are 4s, 4p, and 3d. The nature of the polarization functions for third‐row atoms is reexamined as well. The polarization functions for K, Ca, and Ga through Kr are single set of Cartesian d‐type primitives. The polarization functions for transition metals are defined to be a single 7f set of uncontracted primitives. Comparison with experimental data shows good agreement with bond lengths and angles for representative vapor‐phase metal complexes. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 976–984, 2001
Gas‐phase detection of the HBCC (X1Σ) molecule: a combined crossed beam and computational study of the B(2P)+C2H2(1Σg+) reaction
Authors: Balucani N. , Asvany O. , Lee Y. T. , Kaiser R. I. , Galland N. , Rayez M. T. , Hannachi Y. 
Published: August 2002

AbstractA novel supersonic beam of ground‐state boron atoms [B(2P)] was employed to investigate the reaction of B(2P) with acetylene [C2H2(1Σg+)] at an average collision energy of 16.3±0.4 kJ mol−1 at the most fundamental microscopic level. The crossed molecular beam technique was used to record time of flight spectra at mass to charge ratios of 36 (11BC2H+), 35 (10BC2H+/11BC2+), and 34 (10BC2+) at different laboratory angles. Forward‐convolution fitting of the laboratory data showed that only a product with the gross formula BC2H was formed via a boron versus hydrogen exchange. By combining experimental results with electronic structure calculations, the conclusion was that the reaction proceeded via the initial addition of B(2P) to the two carbon atoms of acetylene, leading to the formation of a first intermediate, the borirene radical (c‐BC2H2). This intermediate underwent various isomerization processes on the BC2H2 potential energy surface before decomposing into the linear HBCC(X1Σ) isomer via a hydrogen atom elimination. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 1359–1365, 2001
Chronic fatigue syndrome and fibromyalgia: Clinical assessment and treatment
Authors: Friedberg Fred , Jason Leonard A. 
Published: August 2002

AbstractChronic fatigue syndrome (CFS) and fibromyalgia (FM) are closely related illnesses of uncertain etiology. This article reviews the research literature on these biobehavioral conditions, with an emphasis on explanatory models, clinical evaluation of comorbid psychiatric disorders, assessment of stress factors, pharmacologic and alternative therapies, and cognitive‐behavioral treatment studies. Furthermore, clinical protocols suitable for professional practice are presented based on an integration of the authors' clinical observations with published data. The article concludes with the recognition that mental health professionals can offer substantial help to these patients. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 433–455, 2001
Multiple sclerosis: Empirical literature for the clinical health psychologist
Authors: Mohr David C. , Cox Darcy 
Published: August 2002

AbstractThis article reviews the empirical literature related to clinical health psychology in multiple sclerosis (MS). MS is a disease in which the immune system attacks the central nervous system. As such, the interactions between medical and psychological variables are complex, and potentially of considerable importance to patients. Common neuropsychological and psychological problems associated with MS and their etiologies are reviewed. The effects of stress and depression on MS exacerbation are discussed, including clinical, immune, endocrine, and neuroimaging findings. The types of coping common in MS and their effects on adjustment are discussed. The empirical literature on psychological and neuropsychological intervention is reviewed. The small literature on caregiving in MS is also summarized. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 479–499, 2001
Treatment of fibromyalgia incorporating EEG‐Driven stimulation: A clinical outcomes study
Authors: Mueller Horst H. , Donaldson C.C. Stuart , Nelson David V. , Layman Mellisa 
Published: August 2002

AbstractThirty patients from a private clinical practice who met the 1990 American College of Rheumatology criteria for fibromyalgia syndrome (FS) were followed prospectively through a brainwave‐based intervention known as electroencephalograph (EEG)‐driven stimulation or EDS. Patients were initially treated with EDS until they reported noticeable improvements in mental clarity, mood, and sleep. Self‐reported pain, then, having changed from vaguely diffuse to more specifically localized, was treated with very modest amounts of physically oriented therapies. Pre‐ to posttreatment and extended follow‐up comparisons of psychological and physical functioning indices, specific FS symptom ratings, and EEG activity revealed statistically significant improvements. EDS appeared to be the prime initiator of therapeutic efficacy. Future research is justified for controlled clinical trials and to better understand disease mechanisms. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 933–952, 2001.
Psychometric properties of goal‐attainment scaling in the assessment of mann's time‐limited psychotherapy
Authors: Shefler Gaby , Canetti Laura , Wiseman Hadas 
Published: August 2002

AbstractThis study tested the reliability and validity of Goal‐Attainment Scaling (GAS) in assessing outcome in Mann's Time‐Limited Psychotherapy (TLP). Judges constructed GAS scales for 33 patients on five dimensions: severity of symptoms, self‐esteem, same‐sex friendships, romantic relationships, and work performance. Patients in the treatment group received 12 sessions of TLP, whereas controls were in a waiting list for the same period of time (12 weeks) before starting time‐limited psychotherapy. Judges rated all patients on GAS before beginning therapy, at termination, and follow‐up. Mean interrater reliability of scores between pairs of judges was r = .88. Convergent validity of the GAS was confirmed by moderate to high correlations with the Health–Sickness Rating Scale (r = .70, p < .001), the Target Complaints Scale (r = .50, p < .01), and the Brief Symptom Inventory (r = .38, p < .05). The findings are discussed and recommendations are made for the reliable and valid use of the GAS in psychotherapy outcome research. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 971–979, 2001.
Validity of the MMPI‐2 basic and Harris–Lingoes subscales in a forensic sample
Authors: Osberg Timothy M. , Poland Danielle L. 
Published: August 2002

AbstractThe present study explored the MMPI‐2 correlates of criminal history in a sample of prison inmates. Although Ma was the only basic scale to demonstrate a significant positive association to crime history, three Harris–Lingoes subscales also bore significant associations to this criterion. The Ma1 (amorality), Pd2 (authority problems), and Pd5 (self‐alienation) subscales demonstrated positive associations with crime history. Hierarchical multiple‐regression analyses were used to examine the incremental validity of the D, Pd, and Ma Harris–Lingoes subscales when added to their parent scales in the prediction of crime history. Only the Pd Harris–Lingoes subscales demonstrated incremental validity in the prediction of this criterion. The value of including the Ma and Pd Harris–Lingoes subscales in criminal risk assessment is discussed. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 1369–1380, 2001.
Comparison of the Mexican and American norms of the MMPI‐2
Authors: Lucio Emilia , Ampudia Amada , Durán Consuelo , León Ivonne , Butcher James N. 
Published: August 2002

AbstractThis article presents the results of the norms for the Mexican general population in comparison with the North American normative population. This study addresses psychopathology from the point of view in which emic and etic traditions are combined. The Mexican sample includes 1744 adults from different regions of the country. Means and standard deviations were calculated and compared with the North‐American normative sample. Cronbach α coefficients also were obtained. The greatest differences observed were in the scales L (lies) and 5 (masculinity–femininity), which indicates that the greater distinctions between both populations are with respect to test‐taking attitude in taking the test, which may be due to cultural factors. In comparison to the American norms, the Mexican population does not show psychopathology on the MMPI‐2 scales. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 1459–1468, 2001.
Potential clients' beliefs about the relative competency and caring of psychologists: Implications for the profession
Authors: Bremer Barbara A. , Foxx Richard M. , Lee Midge , Lykins Dana , Mintz Venus R. , Stine Emily 
Published: August 2002

AbstractTo assess the extent to which the results reported by Warner and Bradley (1991) can be generalized beyond the population of undergraduate psychology students, 132 adults in the metropolitan Harrisburg area were asked to evaluate the competency and traits of clinical psychologists, licensed professional counselors, and psychiatrists. Results indicated that the general public believed counselors to be more caring than psychologists and psychiatrists. All three professional groups were perceived to be comparable in their ability to treat the least severe disorders. Licensed counselors were perceived to be most competent to treat disorders rated by the subjects as moderately severe, comparable in competence to clinical psychologists for adjustment disorders, and comparable to psychiatrists for marital problems. Psychiatrists and psychologists were considered the most competent to treat the most severe disorder, major depression. The results indicate that while clinical psychologists often were viewed as competent, they were not viewed as the single practitioner of choice for any diagnostic classification, nor were they noted to have positive character traits. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 1479–1488, 2001.
Personality and psychopathology profiles of veterans' wives: Measuring distress using the MMPI‐2
Authors: Alessi Marycely W. , Ray JoAnne White , Ray Glen E. , Stewart Scott J. 
Published: August 2002

AbstractThe present study compared personality and psychopathology profiles of veterans' wives against married women in the MMPI‐2 restandardization sample. Differences in levels of distress and pathology were analyzed using the validity, clinical, and content scales of the MMPI‐2. As expected, veterans' wives, when compared to restandardization wives, reported higher levels of psychopathology and distress, with symptoms such as depression, social maladjustment, and other negative, internal symptomatic behaviors. Findings are discussed in terms of the need for additional research examining this “at‐risk” population. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 1535–1542, 2001.
Sense of community in science fiction fandom, Part 2: Comparing neighborhood and interest group sense of community
Authors: Obst Patricia , Zinkiewicz Lucy , Smith Sandy G. 
Published: August 2002

AbstractThere is much debate in community psychology literature as to the dimensions underlying the psychological sense of community (PSOC) construct. One of the few theoretical discussions is that of McMillan and Chavis (1986), who hypothesized four dimensions: Belonging, Fulfillment of Needs, Influence, and Shared Emotional Connection. Debate has also emerged regarding the role of identification within PSOC. However, few studies have explored the place of identification in PSOC. In addition, whereas PSOC has been applied to both communities of interest and geographic communities, to date little research has compared a single group's PSOC with a community of interest to their PSOC with their geographic communities. The current study explored PSOC with participants' interest and geographic communities in a sample (N = 359) of members of science fiction fandom, a community of interest with membership from all over the world. Support emerged for McMillan and Chavis' (1986) four dimensions of PSOC, both within participants' PSOC with their geographic communities and with their community of interest, with the addition of a fifth dimension, that of Conscious Identification. All dimensions emerged as significant predictors of overall sense of community in both community types. Participants reported higher levels of global PSOC with fandom than with their geographic communities, a pattern that also emerged across all factors separately. These results, and implications for PSOC research, are discussed. © 2002 John Wiley & Sons, Inc.
EGFR‐independent activation of ERK1/2 mediates growth inhibition by a PTPase antagonizing K‐vitamin analog
Authors: Kar Siddhartha , Adachi Takahito , Carr Brian I. 
Published: August 2002

AbstractThe K‐vitamin analog Cpd 5 or [2‐(2‐mercaptoethanol)‐3‐methyl‐1,4‐napthoquinone] is a potent cell growth inhibitor in vitro and in vivo, likely due to arylation of enzymes containing a catalytic cysteine. This results in inhibition of protein tyrosine phosphatase (PTPase) activity with resultant hyperphosphorylation of EGF receptors (EGFR) and ERK1/2 protein kinases, which are downstream to EGFR in the MAPK pathway. We used NR6 fibroblast cells, which lack endogenous EGFR and its variant cells transfected with different EGFR mutants to assess the contribution of the EGFR‐mediated signaling pathway to Cpd 5‐mediated ERK activation and cell growth inhibition. Cpd 5 treatment resulted in enhanced phosphorylation of EGFR at carboxyl‐terminal tyrosines. This phosphorylation and activation of EGFR were found to be necessary neither for growth inhibition nor for the activation of the downstream kinases ERK1/2, since both occurred in EGFR‐devoid mutant cells. U0126 and PD 098059, specific inhibitors of MEK1/2, the ERK1/2 kinases, antagonized both cell growth inhibition and ERK1/2 phosphorylation mediated by Cpd5. Cpd 5 was also found to inhibit ERK1/2 phosphatase(s) activity in lysates from all the cells tested, irrespective of their EGFR status. These results show that EGFR‐independent ERK1/2 phosphorylation was involved in the mechanism of Cpd5 mediated growth inhibition. This is likely due to the observed antagonism of ERK phosphatase activity. A candidate PTPase was found to be Cdc25A, a recently identified ERK phosphatase. J. Cell. Physiol. 190: 356–364, 2002. © 2002 Wiley‐Liss, Inc.
Tpl‐2 induces apoptosis by promoting the assembly of protein complexes that contain caspase‐9, the adapter protein Tvl‐1, and procaspase‐3
Authors: Patriotis Christos , Russeva Maria G. , Lin Jun‐Hsiang , Srinivasula Srinivasa M. , Markova Dessislava Z. , Tsatsanis Christos , Makris Antonios , Alnemri Emad S. , Tsichlis Philip N. 
Published: August 2002

AbstractThe Tpl‐2 proto‐oncoprotein promotes cellular proliferation when overexpressed in a variety of tumor cell lines. Here, we present evidence that when overexpressed in immortalized non‐transformed cells, Tpl‐2 induces apoptosis by promoting the activation of caspase‐3 via a caspase‐9‐dependent mechanism, and that apoptosis is enhanced when Tpl‐2 is co‐expressed with the newly identified ankyrin repeat protein Tvl‐1. The activation of caspase‐3 by caspase‐9 is known to depend on the assembly of a multimolecular complex that includes Apaf‐1 and caspase‐9. Data presented here show that co‐expression of Tpl‐2 with Tvl‐1 promotes the assembly of a complex that involves several proteins that bind Apaf‐1 including Tvl‐1, itself, Tpl‐2 and phosphorylated procaspase‐9. More important, procaspase‐3, which under normal growth conditions is not associated with the complex, binds Tvl‐1 conditionally in response to Tpl‐2–generated apoptotic signals. The conditional association of procaspase‐3 with Tvl‐1 promotes the in vivo proteolytic maturation of procaspase‐3 by caspase‐9, a process casually linked to apoptosis. © 2001 Wiley‐Liss, Inc.
Selective reverse micellar extraction of penicillin acylase from E coli
Authors: Gaikar Vilas G , Kulkarni Madhav S 
Published: August 2002

AbstractThe recovery of penicillin acylase from E coli by a new reverse micellar treatment is described. The results are compared with the cell disruption by ultrasound followed by reverse micellar extraction. The process gave selective extraction of penicillin acylase directly from the periplasmic space of E coli without disrupting the cells. Unlike ultrasonication which breaks open the cells entirely, making subsequent processing difficult and expensive, reverse micellar treatment of cells gave a moderate recovery of 60% of enzyme activity in a highly pure form.© 2001 Society of Chemical Industry