bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expedited partner therapy: A multi-component initiative to boost provider counseling
Authors: Markowitz Melissa A. , Ackerman-Banks Christina M. , Oliveira Carlos R. , Fashina Oluwatomini , Pathy Shefali R. , Sheth Sangini S. 
Published: November 2023

Abstract

Background
Expedited partner therapy prescription remains low and highly variable throughout the United States, leading to frequent reinfections with Chlamydia trachomatis and Neisseria gonorrhea. We examined provider counseling on expedited partner therapy before and after an electronic smart tools-based initiative.

Methods
In this quasi-experimental interrupted time series study, we implemented an initiative of electronic smart tools and education for expedited partner therapy in March 2020. We reviewed the records of patients with chlamydia and/or gonorrhea at an urban, academic obstetrics and gynecology clinic in the pre-implementation (March 2019-February 2020) and post-implementation (March 2020-February 2021) groups. Descriptive statistics and an interrupted time-series model were used to compare the percent of expedited partner therapy offered by clinicians to patients in each group.

Results
A total of 287 patient encounters were analyzed, 155 pre-intervention and 132 post-intervention. An increase in expedited partner therapy counseling of 13% (95% CI: 2-24%) was observed pre-intervention (27.1%; 42/155) versus post-intervention (40.2%; 53/132). Significant increases in provider counseling were seen for patients who were single (15%; 95% CI: 3-26%), ≥ 25 years old (21%; 95% CI: 6-37%), receiving public insurance (15%; 95% CI: 3-27%), seen by a registered nurse (18%; 95% CI: 4-32%), or seen for an obstetrics indication (21%; 95% CI: 4-39%). No difference was seen in patients’ acceptance of expedited partner therapy (p = 1.00).

Conclusions
A multi-component initiative focused on electronic smart tools is effective at increasing provider counseling on expedited partner therapy. Further research to understand patient perceptions and acceptance of expedited partner therapy is critical.
Examining Patient Preferences for Express, Telemedicine, and Standard Visits in a Sexual Health Clinic in New York City
Authors: Ratcliffe Judith , Paer Jeffrey , Quigee Daniela , Carnevale Caroline , Richards Paul , Lasota Elijah , Dandan Nadine , Scherer Matthew , Gordon Peter , Cohall Alwyn , Sobieszczyk Magdalena , Zucker Jason 
Published: November 2023

Abstract
This study, completed at an sexually transmitted infection (STI) clinic in 2019 to 2020, evaluated patient preferences for telemedicine, express, and standard visits. Active PrEP users preferred telemedicine and express visits, patients with prior STIs preferred express visits, and cisgender women preferred standard visits. Configuring STI clinic visit types requires shared decision making and individualization.
Congenital and acquired symmastia: a novel correction technique
Authors: Aquino Jonathas Ubiraçaba , Felix Gabriel de Almeida Arruda , Feitosa Roney Gonçalves Fechine , Tumeh Rafael Alves , Neto Miguel Sabino 
Published: November 2023

Background:
Symmastia, first defined by Spence in 1984, is a medical condition characterized by the convergence of breast tissues in the midline, resulting in the absence of adhesion between the skin and subcutaneous tissue over the presternal area. It can be either congenital or acquired. Currently, there is no universally accepted treatment for symmastia. This study presents a surgical approach, preoperative, and postoperative care for the correction of symmastia.

Methods:
Between January 2014 and December 2020, a surgical technique was performed on 100 patients, including 59 with congenital symmastia and 41 with acquired symmastia. The technique involved creating a thin skin flap and attaching it to the sternum to reconstruct the intermammary V-shaped region.

Results:
All patients in both groups underwent subglandular implant placement, with textured/polyurethane prostheses used in 97% of cases. Three cases did not use any implants. In the corrective surgery, patients received smaller, round implants, following the steps of the surgical approach devised by the author. The median satisfaction scores were high in both groups.

Conclusion:
The surgical approach described in this study is a safe, one-time procedure with a low risk of complications and high patient satisfaction. The technique is reproducible and provides consistent outcomes.
An Investigation Into Whether the Facial Nerve and Auditory Nerve can be Protected by Removal of the Posterior Wall of the Internal Auditory Canal Under 30° Neuroendoscopy During Vestibular Schwannoma Surgery
Authors: Yang Siming , Wang Jianbiao , Yang Chi , Li Zhengyuan , Qiao Yang , Wang Cunzhi , Wang Jingtao , Hong Wenming , Wang Bin 
Published: November 2023

The aim of this study was to evaluate the surgical technique of microresection of vestibular schwannoma by removing the posterior wall of the internal auditory canal (IAC) under neuroelectrophysiological monitoring and 30° neuroendoscopy, with respect to the protection of facial and auditory nerve function. Forty-five cases of microscopic resection of auditory neuromas were performed through a posterior approach to the inferior occipital sigmoid sinus using a 30° neuroendoscope to assist in the removal of the posterior wall of the IAC during surgery. Patients underwent cranial enhancement magnetic resonance imaging examination and functional assessment of the facial and auditory nerves before and after surgery, and clinical data were collected for retrospective analysis. All tumors were removed in 41 patients, and most of the tumors were removed in 4 patients. The facial nerve was anatomically preserved in 43 patients (95.6%), and the percentage of facial nerve function preservation (House-Brackmann grade I–II) was 84.4%. Forty patients (88.9%) had anatomical preservation of the auditory nerve, with a 66.7% functional preservation rate. At 3 to 39 months of follow-up, 45 patients were reviewed with 3.0 T-enhanced magnetic resonance imaging, and no tumor recurrence was observed in any of the patients. Microscopic resection of auditory neuroma through the posterior approach of the inferior occipital sigmoid sinus with intraoperative use of 30° neuroendoscopic assistance to abrade the posterior wall of the IAC can eliminate dead space in certain anatomical areas during surgery and minimize surgical damage to the facial and auditory nerves, which is the basis for preservation of facial and auditory nerve function.
Staphylococcus aureus associated with post-operative wound infections in Western Kenya reveals genomic hotspots for pathogen evolution
Authors: MOGOI NICHOLAS NYABERA , Sifuna Anthony Wawire , Okoth Patrick Kirsteen , Reva Oleg , Malaba Rose , Negesa Ruth , Nyongesa Peter Kuloba , Osoro Ezra Kombo , Welch Martin 
Published: November 2023

Staphylococcus aureus is one of the most common pathogens associated with hospital infections. Although S. aureusinfections have been well studied in developed countries, far less is known about the biology of the pathogen in sub-Saharan Africa. Here, we report on the isolation, antibiotic resistance profiling, whole genome sequencing, and genome comparison of six multi-drug resistant isolates of S. aureus obtained from a referral hospital in Kakamega, western Kenya. Five of the six isolates contained a 20.7-kb circular plasmid carrying blaZ (associated with resistance to b-lactam antibiotics). These five strains all belonged to the same sequence type, ST152. Despite the similarity of the plasmid these isolates, whole genome sequencing revealed that the strains differed, depending on whether they were associated with hospital-acquired or community-acquired infections. The most striking difference was the larger number of horizontally-acquired genomic islands (GIs) in the nosocomial isolates. The data indicate that adaptation to the hospital environment may also be linked with mutations in the uvrAB genes.
Unveiling Urban Complexity: Research note on integrating OpenStreetMap to enhance representation of fine-scale landscape heterogeneity
Authors: Gelmi-Candusso Tiziana A. , Rodriguez Peter , Fortin Marie-Josée 
Published: November 2023

AbstractLandscape heterogeneity has an impact on wildlife behavior, their interactions, and their persistence. Urban landscapes are among the world’s most heterogeneous landscapes, yet current global landcover maps classify developed land in a single landcover type. This limits the spatial scale at which urban ecologists can approach research questions. OpenStreetMap (OSM), an open-source mapping platform, can be leveraged to enhance the representation of landscape heterogeneity in developed areas. For this, we extracted OSM features with attributes representing infrastructure, land use and green cover, integrating these into a continental landcover map through a globally applicable computational framework. We validated our OSM-enhanced landcover layer against existing remote sensing, aerial photography, and local governmental maps for 33 cities in North America. Our framework’s output provides an 89% accurate representation of landscape heterogeneity. We discuss caveats, potential improvements, and ecological applications. Our OSM-based landcover enhancement framework will facilitate the use of open-source landscape information for improved ecological modeling and urban planning.
A simple and reliable method for claustrum localization across age in mice
Authors: Shaker Tarek , Dagpa Gwyneth J. , Cattaud Vanessa , Marriott Brian A. , Sultan Mariam , Almokdad Mohammed , Jackson Jesse 
Published: November 2023

1.ABSTRACTThe anatomical organization of the rodent claustrum remains obscure due to lack of clear borders that distinguish it from neighboring forebrain structures. Defining what constitutes the claustrum is imperative for elucidating its functions. Methods based on gene/protein expression or transgenic mice have been used to spatially outline the claustrum but often report incomplete labeling and/or lack of specificity during certain neurodevelopmental timepoints. To reliably identify claustrum cells in mice, we propose a simple immunolabelling method that juxtaposes the expression pattern of claustrum-enriched and cortical-enriched markers. We determined that claustrum cells immunoreactive for the claustrum-enriched markers Nurr1 and Nr2f2 are devoid of the cortical marker Tle4, which allowed us to differentiate the claustrum from adjoining cortical cells. Using retrograde tracing, we verified that nearly all claustrum projection neurons lack Tle4 but expressed Nurr1/Nr2f2 markers to different degrees. At neonatal stages between 7 and 21 days, claustrum projection neurons were identified by their Nurr1-postive/Tle4-negative expression profile, a time-period when techniques used to localize the claustrum in adult mice are ineffective. Finally, exposure to environmental novelty enhanced the expression of the neuronal activation marker cFos in the claustrum region. Notably, cFos labeling was mainly restricted to Nurr1-positive cells and nearly absent from Tle4-positive cells, thus corroborating previous work reporting novelty-induced claustrum activation. Taken together, this method will aid in studying the claustrum during postnatal development and may improve histological and functional studies where other approaches are not amenable.
Microvascular engineering for the development of a non-embedded liver sinusoid with a lumen: when endothelial cells do not lose their edge
Authors: Monroy-Romero Ana Ximena , Nieto-Rivera Brenda , Xiao Wenjin , Hautefeuille Mathieu 
Published: November 2023

AbstractMicrovascular engineering seeks to exploit known cell-cell and cell-matrix interactions in the context of vasculogenesis to restore homeostatic or disease development of reliable capillary modelsin vitro. However, current systems generally focus on recapitulating microvessels embedded in thick gels of extracellular matrix, overlooking the significance of discontinuous capillaries, which play a vital role in tissue-blood exchanges particularly in organs like the liver. In this work, we introduce a novel method to stimulate the spontaneous organization of endothelial cells into non-embedded microvessels. By creating an anisotropic micropattern at the edge of a development-like matrix dome using Marangoni-flow, we achieved a long, non-random orientation of endothelial cells, laying a premise for stable lumenized microvessels. Our findings revealed a distinctive morphogenetic process leading to mature lumenized capillaries, demonstrated with both murine and human immortalized liver sinusoidal endothelial cell lines (LSECs). The progression of cell migration, proliferation and polarization was clearly guided by the pattern, initiating the formation of a multicellular cord that caused a deformation spanning extensive regions and generated a wave-like folding of the gel, hinged at a laminin depleted zone, enveloping the cord with gel proteins. This event marked the onset of lumenogenesis, regulated by the gradual apico-basal polarization of the wrapped cells, leading to the maturation of vessel tight junctions, matrix remodeling, and ultimately the formation of a lumen—recapitulating the development of vesselsin vivo. Furthermore, we demonstrate that the process strongly relies on the initial gel edge topography, while the geometry of the vessels can be tuned, from a curved to a straight structure. We believe our facile engineering method, guiding an autonomous self-organization of vessels without the need for supporting cells or complex prefabricated scaffolds, holds promise for future integration into microphysiological systems featuring discontinuous, fenestrated capillaries.Graphical abstract
Oligo-barcodes illuminate holocentric karyotype evolution inRhynchospora(Cyperaceae)
Authors: Mata-Sucre Yennifer , Parteka Leticia Maria , Ritz Christiane , Gatica-Arias Andrés , Félix Leonardo P. , Thomas Willian Wayt , Souza Gustavo , Vanzela André L. L. , Pedrosa-Harand Andrea , Marques André 
Published: November 2023

ABSTRACTHolocentric karyotypes are assumed to rapidly evolve through chromosome fusions and fissions due to the diffuse nature of their centromeres. Here, we took advantage of the recent availability of a chromosome-scale reference genome forRhynchospora breviuscula, a model species of this holocentric genus, and developed the first set of oligo-based barcode probes for a holocentric plant. These probes were applied to 13 additional species of the genus, aiming to investigate the evolutionary dynamics driving the karyotype evolution inRhynchospora. The two sets of probes were composed of 27,392 (green) and 23,968 (magenta) oligonucleotides, and generated 15 distinct FISH signals as a unique barcode pattern for the identification of all five chromosome pairs of theR. breviusculakaryotype. Oligo-FISH comparative analyzes revealed different types of rearrangements, such as fusions, fissions, putative inversions and translocations, as well as genomic duplications among the analyzed species. Two rounds of whole genome duplication (WGD) were demonstrated inR. pubera, but both analyzed accessions differed in the complex chain of events that gave rise to its large, structurally diploidized karyotypes with 2n= 10 or 12. Considering the phylogenetic relationships and divergence time of the species, the specificity and synteny of the probes were maintained up to species with a divergence time of ∼25 My. However, karyotype divergence in more distant species hindered chromosome mapping and the inference of specific events. This barcoding system is a powerful tool to study chromosomal variations and genomic evolution in holocentric chromosomes ofRhynchosporaspecies.
Towards a Biohybrid Lung? Impacted Endothelial Layer Integrity in Inflammatory Conditions
Authors: Cheremkhina Maria , Babendreyer Aaron , Neullens Christopher T. , Krapp Susanne , Pabst Alessa , Ohl Kim , Tenbrock Klaus , Ruetten Stephan , Ludwig Andreas , Cornelissen Christian G. , Thiebes Anja Lena , Jockenhoevel Stefan 
Published: November 2023

AbstractLimited hemocompatibility, inflammation, and sepsis are frequent complications during extracorporeal membrane oxygenation (ECMO). Endothelialisation of gas exchange membranes has been proposed to overcome these limitations and general feasibility has been demonstrated. However, these investigations used biohybrid devices under standardin vitroculture conditions neglecting patients’ inflammatory status in clinics. In this study, we investigate human umbilical vein endothelial cell (HUVEC) behaviour on gas exchange membranes under inflammatory conditions in a microfluidic model system using immunocytochemistry, scanning electron microscopy, flow cytometry, and qPCR. While co-culture with healthy control peripheral blood mononuclear cells (PBMCs) does not change endothelial layer integrity, confluence of the endothelial layer is substantially reduced upon inflammation via lipopolysaccharide (LPS) activated PBMCs. Cell adhesion molecules are increasingly expressed under inflammatory conditions, consistent with an increased leukocyte adhesion. An upregulation of several genes linked to inflammation is observed: ICAM-1, VCAM-1, E-Selectin, IL6, IL8, IL10, and MCP-1. Our findings suggest that endothelial cells struggle to maintain their layer integrity within a biohybrid device when exposed to inflammatory conditions. This raises the question of whether endothelialisation is an effective advancement of current technologies considering the frequent inflammatory status in ECMO patients. Yet the presented setup qualifies as sepsisin vitromodel replicating the physiological vascular leak phenomenon to aid future investigations in biohybrid lung research.
Ubiquitin protease Ubp1 cooperates with Ubp10 and Ubp12 to revert Lysine-164 PCNA ubiquitylation at replication forks
Authors: Zamarreño Javier , Bueno Avelino , Sacristán María P. 
Published: November 2023

ABSTRACTProliferating cell nuclear antigen (PCNA) is essential for the faithful duplication of eukaryotic genomes. PCNA orchestrates events necessary to deal with threats to genomic integrity, such as the DNA damage tolerance (DDT) response. DDT is a mechanism by which eukaryotic cells bypass replication-blocking lesions to prevent replisome instability. DDT pathways are regulated by the ubiquitylation of PCNA and the consequent recruitment of specialized polymerases and mechanisms able to guarantee the continuity of replication. We have previously described that the deubiquitylases Ubp10 and Ubp12 associate with replication forks and modulate DDT events by reverting the ubiquitylation of PCNA inSaccharomyces cerevisiae. The results of this study unveil Ubp1 as a new PCNA deubiquitylase, which cooperates with Ubp10 and Ubp12 in the regulation of DDT during DNA replication. Ubp1 is known as a cytoplasmic protein, however, we found that it also localizes to the nucleus where it binds to chromatin and associates with DNA replication forks. In addition, Ubp1 interacts with and deubiquitylates PCNA. The ablation of Ubp1, Ubp10, and Ubp12 enhances both the accumulation of ubiquitylated PCNA and the DNA replication defects observed in cells depleted for Ubp10 and Ubp12, supporting a cooperative role among the three enzymes.IMPORTANCEPCNA ubiquitylation regulates DDT mechanisms to bypass genotoxic lesions during replication and that PCNA deubiquitylation is required to limit the extent of bypass events. This study shows thatSaccharomyces cerevisiaePCNA is ubiquitylated during an unperturbed S-phase progression and that three ubiquitin proteases (Ubp1, Ubp10, and Ubp12) work together facilitating DNA replication by efficiently controlling ubiquitylation of PCNA at replication forks.
Lymphatic Dysfunction Models an Autoimmune Emphysema Phenotype of Chronic Obstructive Pulmonary Disease
Authors: Summers Barbara , Kim Kihwan , Lu Tyler M. , Houghton Sean , Trivedi Anjali , Quintero Joselyn Rojas , Cala-Garcia Juan , Pannellini Tania , Polverino Francesca , Lis Raphaël , Reed Hasina Outtz 
Published: November 2023

AbstractChronic Obstructive Pulmonary Disease (COPD) is a heterogeneous disease that is characterized by many clinical phenotypes. One such phenotype of COPD is defined by emphysema, pathogenic lung tertiary lymphoid organs (TLOs), and autoantibody production. We have previously shown that lymphatic dysfunction can cause lung TLO formation and lung injury in mice. We now sought to uncover whether underlying lymphatic dysfunction may be a driver of lung injury in cigarette smoke (CS)-induced COPD. We found that lung TLOs in mice with lymphatic dysfunction produce autoantibodies and are associated with a lymphatic endothelial cell subtype that expresses antigen presentation genes. Mice with underlying lymphatic dysfunction develop increased emphysema after CS exposure, with increased size and activation of TLOs. CS further increased autoantibody production in mice with lymphatic dysfunction. B-cell blockade prevented TLO formation and decreased lung injury after CS in mice with lymphatic dysfunction. Using tissue from human COPD patients, we also found evidence of a lymphatic gene signature that was specific to patients with emphysema and prominent TLOs compared to COPD patients without emphysema. Taken together, these data suggest that lymphatic dysfunction may underlie lung injury in a subset of COPD patients with an autoimmune emphysema phenotype.
Dissociating Goal from Outcome During Action Observation
Authors: Liu Shuchen , Wurm Moritz F. , Caramazza Alfonso 
Published: November 2023

ABSTRACTUnderstanding the goal of an observed action requires computing representations that are invariant to specific instantiations of the action. For example, we can accurately infer the goal of an action even when the agent’s desired outcome is not achieved. Observing actions consistently recruits a set of frontoparietal and posterior temporal regions, often labeled the "action observation network" (AON). While progress has been made in charting which regions of the AON, are involved in understanding goals of observed actions, it is not clear where goals are represented independently of outcomes. We used fMRI-based multivariate pattern analysis to identify such regions. Participants watched videos of successful and failed attempts of actions with different goals involving 2 different object types. We found that bilateral anterior inferior parietal lobe and right ventral premotor cortex distinguished between object-specific action goals regardless of outcomes. Left anterior inferior parietal lobe encodes action goals regardless of both outcomes and object types. Our results provide insights into the neural basis of representing action goals and the different roles of frontoparietal and posterior temporal regions in action understanding.