bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of maternal oral microbiome on newborn oral microbiome in healthy pregnancies
Authors: Russo Monica , Calevo Maria Grazia , D’Alessandro Gloria , Tantari Matteo , Migliorati Marco , Piccardo Ilaria , Perucchin Paola Polo , Arioni Cesare 
Published: October 2023

Abstract
Background
Periodontal disease and its bacteria can be responsible for pregnancy complications and transmission of periodontal bacteria from mother to newborn.

Methods
A salivary swab to 60 healthy, full-term newborns and their mothers was taken immediately after birth. The test was performed with Real Time PCR method to evaluate the expression of the gene through DNA amplification. The species considered were: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum ssp.

Results
The newborn oral microbiome was composed primarily by saprophytes (98.38 + 4.88%), just like the mothers (98.8 + 3.69%). There was a statistically significant difference of the total microbiological density in newborns and mothers (p = 0.0001). Maternal and neonatal oral microbiome had a correlated total microbiological density only in 33.3% (N = 20/60) of cases. The analysis of the oral microbiome showed a pathological composition only in 12/60 babies (20%). The most frequent detected specie in newborns was Fusobacterium nucleatum (9/12 babies, 75%), as well as for the mothers (53.3%). However, the pathogen was present both in baby and his mother only in 3 dyads. Porphyromonas gingivalis showed the highest association mother-baby (4/12 dyads, 33%). Porphyromonas gingivalis was the pathogen with the highest bacterial load in the 12 mothers. We found a statistically significant difference in the total load of Porphyromonas gingivalis in mothers and babies (p = 0.02).

Conclusions
There was a statistically significant difference in the richness of the microbiome from newborns and mothers. Even comparing the microbiological density in the oral cavity of the individual mother-child pairs, we did not find a significant concordance. These results seem to suggest a low influence of maternal oral microbiome on the richness of the oral neonatal one. We didn’t find mother-child concordance (p = 0.0001) in the presence of pathogenic periodontal micro-organisms. Fusobacterium nucleatum was the most frequent specie detected. Porphyromonas gingivalis instead was the bacteria with the higher possibility of transmission. In conclusion in our study maternal oral health doesn’t affect healthy, full-term newborns’ oral microbiome. Further studies are needed to understand the maternal influence on newborn’s oral microbiome and its effects on babies long-term health.
Maternal gestational diabetes mellitus and the childhood asthma in offspring: a meta-analysis
Authors: Huang Xufeng , Huang Zhengguo , Zhang Jing , Jiang You 
Published: October 2023

Abstract
Background
Maternal diabetes might be related to a high risk of allergic disease in offspring. However, it remains unknown if maternal gestational diabetes mellitus (GDM) is also associated with a high incidence of childhood asthma in offspring. A systematic review and meta-analysis was performed to investigate the above association.

Methods
Relevant observational studies were obtained by search of electronic databases including Medline, Embase, Cochrane Library, and Web of Science. A randomized-effects model was selected to pool the data by incorporating the influence of potential heterogeneity. The Newcastle-Ottawa Scale was used for study quality evaluation. Subgroup analyses were performed to evaluate the potential influences of study characteristics on the outcome.

Results
Ten datasets from seven moderate to high quality cohort studies, involving 523,047 mother-child pairs were included in the meta-analysis. Overall, maternal GDM was associated with a higher risk of childhood asthma in offspring (risk ratio [RR]: 1.22, 95% confidence interval [CI]: 1.07 to 1.39, p = 0.003; I2 = 30%). Subgroup analyses showed that the association was not significantly affected by study design, validation methods for GDM, or diagnostic strategy for asthma (p for subgroup analyses all > 0.05). The association between maternal GDM and asthma in offspring was more remarkable after adjusting maternal body mass index in early pregnancy (RR: 1.50 versus 1.06, p < 0.001), but significantly weakened after adjusting hypertensive disorders during pregnancy (RR: 1.08 versus 1.50, p = 0.001).

Conclusions
Maternal GDM may be associated with an increased incidence of childhood asthma in offspring.
Image quality in whole-body MRI using the MY-RADS protocol in a prospective multi-centre multiple myeloma study
Authors: Keaveney Sam , Dragan Alina , Rata Mihaela , Blackledge Matthew , Scurr Erica , Winfield Jessica M. , Shur Joshua , Koh Dow-Mu , Porta Nuria , Candito Antonio , King Alexander , Rennie Winston , Gaba Suchi , Suresh Priya , Malcolm Paul , Davis Amy , Nilak Anjumara , Shah Aarti , Gandhi Sanjay , Albrizio Mauro , Drury Arnold , Pratt Guy , Cook Gordon , Roberts Sadie , Jenner Matthew , Brown Sarah , Kaiser Martin , Messiou Christina 
Published: October 2023

Abstract
Background
The Myeloma Response Assessment and Diagnosis System (MY-RADS) guidelines establish a standardised acquisition and analysis pipeline for whole-body MRI (WB-MRI) in patients with myeloma. This is the first study to assess image quality in a multi-centre prospective trial using MY-RADS.

Methods
The cohort consisted of 121 examinations acquired across ten sites with a range of prior WB-MRI experience, three scanner manufacturers and two field strengths. Image quality was evaluated qualitatively by a radiologist and quantitatively using a semi-automated pipeline to quantify common artefacts and image quality issues. The intra- and inter-rater repeatability of qualitative and quantitative scoring was also assessed.

Results
Qualitative radiological scoring found that the image quality was generally good, with 94% of examinations rated as good or excellent and only one examination rated as non-diagnostic. There was a significant correlation between radiological and quantitative scoring for most measures, and intra- and inter-rater repeatability were generally good.
When the quality of an overall examination was low, this was often due to low quality diffusion-weighted imaging (DWI), where signal to noise ratio (SNR), anterior thoracic signal loss and brain geometric distortion were found as significant predictors of examination quality.

Conclusions
It is possible to successfully deliver a multi-centre WB-MRI study using the MY-RADS protocol involving scanners with a range of manufacturers, models and field strengths. Quantitative measures of image quality were developed and shown to be significantly correlated with radiological assessment. The SNR of DW images was identified as a significant factor affecting overall examination quality.

Trial registration
ClinicalTrials.gov, NCT03188172, Registered on 15 June 2017.

Critical relevance statement
Good overall image quality, assessed both qualitatively and quantitatively, can be achieved in a multi-centre whole-body MRI study using the MY-RADS guidelines.

Key points
• A prospective multi-centre WB-MRI study using MY-RADS can be successfully delivered.
• Quantitative image quality metrics were developed and correlated with radiological assessment.
• SNR in DWI was identified as a significant predictor of quality, allowing for rapid quality adjustment.

Graphical Abstract
Higher field reduced FOV diffusion-weighted imaging for abdominal imaging at 5.0 Tesla: image quality evaluation compared with 3.0 Tesla
Authors: Zhang Yunfei , Sheng Ruofan , Yang Chun , Dai Yongming , Zeng Mengsu 
Published: October 2023

Abstract
Objective
To evaluate the image quality of reduced field-of-view (rFOV) DWI for abdominal imaging at 5.0 Tesla (T) compared with 3.0 T.

Methods
Fifteen volunteers were included into this prospective study. All the subjects underwent the 3.0 T and 5.0 T MR examinations (time interval: 2 ± 1.9 days). Free-breathing (FB), respiratory-triggered (RT), and navigator-triggered (NT) spin-echo echo-planner imaging-based rFOV-DWI examinations were conducted at 3.0 T and 5.0 T (FB3.0 T, NT3.0 T, RT3.0 T, FB5.0 T, NT5.0 T, and RT5.0 T) with two b values (b = 0 and 800 s/mm2), respectively. The signal-to-noise ratio (SNR) of different acquisition approaches were determined and statistically compared. The image quality was assessed and statistically compared with a 5-point scoring system.

Results
The SNRs of any 5.0 T DWI images were significantly higher than those of any 3.0 T DWI images for same anatomic locations. Moreover, 5.0 T rFOV-DWIs had the significantly higher sharpness scores than 3.0 T rFOV-DWIs. Similar distortion scores were observed at both 3.0 T and 5.0 T. Finally, RT5.0 T displayed the best overall image quality followed by NT5.0 T, FB5.0 T, RT3.0 T, NT3.0 T and FB3.0 T (RT5.0 T = 3.9 ± 0.3, NT5.0 T = 3.8 ± 0.3, FB5.0 T = 3.4 ± 0.3, RT3.0 T = 3.2 ± 0.4, NT3.0 T = 3.1 ± 0.4, and FB3.0 T = 2.7 ± 0.4, p < 0.001).

Conclusion
The 5.0 T rFOV-DWI showed better overall image quality and improved SNR compared to 3.0 T rFOV-DWI, which holds clinical potential for identifying the abdominal abnormalities in routine practice.

Critical relevance statement
This study provided evidence that abdominal 5.0 Tesla reduced field of view diffusion-weighted imaging (5.0 T rFOV-DWI) exhibited enhanced image quality and higher SNR compared to its 3.0 Tesla counterparts, holding clinical promise for accurately visualizing abdominal abnormalities.

Key points
• rFOV-DWI was firstly integrated with high-field-MRI for visualizing various abdominal organs.
• This study indicated the feasibility of abdominal 5.0 T-rFOV-DWI.
• Better image quality was identified for 5.0 T rFOV-DWI.

Graphical Abstract
Hip MRI in flexion abduction external rotation for assessment of the ischiofemoral interval in patients with hip pain—a feasibility study
Authors: Heimann Alexander F. , Walther Jonas , Tannast Moritz , Schwab Joseph M. , Wagner Moritz , Brunner Alexander , Lerch Till D. , Steppacher Simon D. , Vavron Peter , Schmaranzer Ehrenfried , Schmaranzer Florian 
Published: October 2023

Abstract
Objectives
To assess the feasibility of flexion-abduction-external rotation (FABER) magnetic resonance imaging (MRI) of the hip to visualize changes in the ischiofemoral interval and ability to provoke foveal excursion over the acetabular rim.

Methods
IRB-approved retrospective single-center study. Patients underwent non-contrast 1.5-T hip MRI in the neutral and FABER position. Two readers measured the ischiofemoral interval at three levels: proximal/distal intertrochanteric distance and ischiofemoral space. Subgroup analysis was performed for hips with/without high femoral torsion, or quadratus femoris muscle edema (QFME), respectively. A receiver operating curve with calculation of the area under the curve (AUC) for the prediction of QFME was calculated. The presence of foveal excursion in both positions was assessed.

Results
One hundred ten patients (121 hips, mean age 34 ± 11 years, 67 females) were evaluated. FABER-MRI led to narrowing (both p < .001) of the ischiofemoral interval which decreased more at the proximal (mean decrease by 26 ± 7 mm) than at the distal (6 ± 7 mm) intertrochanteric ridge. With high femoral torsion/ QFME, the ischiofemoral interval was significantly narrower at all three measurement locations compared to normal torsion/no QFME (p < .05). Accuracy for predicting QFME was high with an AUC of .89 (95% CI .82–.94) using a threshold of ≤ 7 mm for the proximal intertrochanteric distance.
With FABER-MRI foveal excursion was more frequent in hips with QFME (63% vs 25%; p = .021).

Conclusion
Hip MRI in the FABER position is feasible, visualizes narrowing of the ischiofemoral interval, and can provoke foveal excursion.

Critical relevance statement
FABER MRI may be helpful in diagnosing ischiofemoral impingement and detecting concomitant hip instability by overcoming shortcomings of static MR protocols that do not allow visualization of dynamic changes in the ischiofemoral interval and thus may improve surgical decision making.

Key points
• FABER MRI enables visualization of narrowing of the ischiofemoral interval proximal to the lesser trochanter.
• Proximal intertrochanteric distance of ≤ 7 mm accurately predicts quadratus femoris muscle edema.
• Foveal excursion was more frequent in hips with quadratus femoris muscle edema.

Graphical Abstract
Severe thrombocytopenia induced by tirofiban after percutaneous coronary intervention: a case report
Authors: Wang Ze-Mu , Wang Bin , Li Ya-Fei , Chen Bei , Shen Qin , Li Dian-Fu , Wang Lian-Sheng 
Published: October 2023

Abstract
Background
Tirofiban is a nonpeptide glycoprotein IIb/IIIa receptor antagonist used widely in patients subjected to percutaneous coronary intervention. While the usage of tirofiban sets an important clinical benefit, severe thrombocytopenia can occur with use of this agent.

Case presentation
A 76-year-old Chinese man was admitted with 1-month history of sudden onset of chest tightness. He was diagnosed as having subacute inferior myocardial infarction, and percutaneous coronary intervention was performed. After the procedure, patient received tirofiban at 0.15 µg/kg/minute for 4 h. A blood sample was obtained for a complete blood count; severe thrombocytopenia was reported according to routine orders at our hospital. All antiplatelet drugs including tirofiban, aspirin, and clopidogrel were immediately discontinued. The patient received platelet transfusions and was treated with immunoglobulin G. Two days later, the patient’s platelet count had increased to 75 × 109/L. There was a significant improvement after day 5, and the platelet count was 112 × 109/L. Seven days after the acute thrombocytopenia, he was discharged with normal platelet count.

Conclusions
Clinicians should be particularly aware of tirofiban-induced thrombocytopenia in routine practice.
Enhancing cord blood stem cell-derived NK cell growth and differentiation through hyperosmosis
Authors: Wen Wei , Chen Xiang , Shen Xin-Yi , Li Hua-Yu , Zhang Feng , Fang Feng-Qi , Zhang Xiao-Bing 
Published: October 2023

Abstract
Background
Natural killer (NK) cells hold great promise in treating diverse hematopoietic and solid tumors. Despite their availability from peripheral blood and cord blood, stem cell-derived NK cells offer an 'off-the-shelf' solution. Hematopoietic stem and progenitor cells (HSPCs) derived from cord blood pose no risk to the newborn or mother and are virtually ideal sources for NK cell differentiation.

Methods
We developed a modified protocol to differentiate HSPCs to NK cells under serum-free conditions using defined factors. The HSPC-derived NK (HSC-NK) cells could be expanded in a K562 feeder cell-dependent manner. Furthermore, using lentivirus transduction, chimeric antigen receptor (CAR)-modified HSPCs could be differentiated into NK cells, leading to the establishment of CAR-NK cells.

Results
The efficiency of NK cell differentiation from HSPCs was increased through the simple modulation of osmotic pressure by the addition of sodium chloride or glucose. Furthermore, the hyperosmosis-primed HSC-NK cells exhibited enhanced proliferation capacity and maintained normal functional characteristics, including transcriptome and antitumor efficacy. The optimized protocol yielded approximately 1.8 million NK cells from a single CD34-positive cell within a 28-day cycle, which signifies more than a ten-fold increase in efficiency relative to the conventional methods. This optimized protocol was also suitable for generating CAR-NK cells with high yields compared to standard conditions.

Conclusions
The results of this study establish high osmotic pressure as a simple yet powerful adjustment that significantly enhances the efficiency and functionality of HSC-NK cells, including CAR-NK cells. This optimized protocol could lead to cost-effective, high-yield NK cell therapies, potentially revolutionizing cancer immunotherapy strategies.
Novel therapeutic strategies for injured endometrium: intrauterine transplantation of menstrual blood‑derived cells from infertile patients
Authors: Hosoya Satoshi , Yokomizo Ryo , Kishigami Harue , Fujiki Yukiko , Kaneko Erika , Amita Mitsuyoshi , Saito Takakazu , Kishi Hiroshi , Sago Haruhiko , Okamoto Aikou , Umezawa Akihiro 
Published: October 2023

Abstract
Background
Menstrual blood-derived cells show regenerative potential as a mesenchymal stem cell and may therefore be a novel stem cell source of treatment for refractory infertility with injured endometrium. However, there have been few pre-clinical studies using cells from infertile patients, which need to be addressed before establishing an autologous transplantation. Herein, we aimed to investigate the therapeutic capacity of menstrual blood-derived cells from infertile patients on endometrial infertility.

Methods
We collected menstrual blood-derived cells from volunteers and infertile patients and confirmed their mesenchymal stem cell phenotype by flow cytometry and induction of tri-lineage differentiation. We compared the proliferative and paracrine capacities of these cells. Furthermore, we also investigated the regenerative potential and safety concerns of the intrauterine transplantation of infertile patient-derived cells using a mouse model with mechanically injured endometrium.

Results
Menstrual blood-derived cells from both infertile patients and volunteers showed phenotypic characteristics of mesenchymal stem cells. In vitro proliferative and paracrine capacities for wound healing and angiogenesis were equal for both samples. Furthermore, the transplantation of infertile patient-derived cells into uterine horns of the mouse model ameliorated endometrial thickness, prevented fibrosis, and improved fertility outcomes without any apparent complications.

Conclusions
In our pre-clinical study, intrauterine transplantation of menstrual blood-derived cells may be a novel and attractive stem cell source for the curative and prophylactic therapy for injured endometrium. Further studies will be warranted for future clinical application.
An Empirical Model to Predict Chloride Penetrations in Concrete Containing Palm Oil Fuel Ash Based on 10-Year Exposure Under Marine Environment
Authors: Chalee Wichian , Jaiyong Amornchai , Cheewaket Tieng , Jaturapitakkul Chai 
Published: October 2023

AbstractThe main objective of this study is to develop an empirical model for predicting the chloride penetration into concrete exposed to the sea water along shorelines. Concrete mixed with 0–50% of palm oil fuel ash, using W/B ratio of 0.40–0.50 were examined. Available database of concrete samples under the sea water submersion at 1, 3, 5, 7 and 10 years was collected. The study found that the developed empirical model could predict total chloride content in palm oil fuel ash concrete at any position below the concrete surface, which had been submerged under sea water for 1 year and over. The W/B ratio is also limited between 0.40 and 0.50. Application of this model is valid in the situation where penetration of chloride is in one direction. It was also shown that the margin of errors in this study is within ± 35% range when compared to the results presented by other researchers.
Controversies regarding lithium-associated weight gain: case–control study of real-world drug safety data
Authors: Greil Waldemar , de Bardeci Mateo , Müller-Oerlinghausen Bruno , Nievergelt Nadja , Stassen Hans , Hasler Gregor , Erfurth Andreas , Cattapan Katja , Rüther Eckart , Seifert Johanna , Toto Sermin , Bleich Stefan , Schoretsanitis Georgios 
Published: October 2023

Abstract
Background
The impact of long-term lithium treatment on weight gain has been a controversial topic with conflicting evidence. We aim to assess reporting of weight gain associated with lithium and other mood stabilizers compared to lamotrigine which is considered free of metabolic adverse drug reactions (ADRs).

Methods
We conducted a case/non-case pharmacovigilance study using data from the AMSP project (German: “Arzneimittelsicherheit in der Psychiatrie”; i.e., Drug Safety in Psychiatry), which collects data on ADRs from patients treated in psychiatric hospitals in Germany, Austria, and Switzerland. We performed a disproportionality analysis of reports of weight gain (> 10% of baseline body weight) calculating reporting odds ratio (ROR). We compared aripiprazole, carbamazepine, lithium, olanzapine, quetiapine, risperidone, and valproate to lamotrigine. Additional analyses related to different mood stabilizers as reference medication were performed. We also assessed sex and age distributions of weight-gain reports.

Results
We identified a total of 527 cases of severe drug-induced weight gain representing 7.4% of all severe ADRs. The ROR for lithium was 2.1 (95%CI 0.9–5.1, p > 0.05), which did not reach statistical significance. Statistically significant disproportionate reporting of weight gain was reported for olanzapine (ROR: 11.5, 95%CI 4.7–28.3, p < 0.001), quetiapine (ROR: 3.4, 95%CI 1.3–8.4, p < 0.01), and valproate (ROR: 2.4, 95%CI 1.1–5.0, p = 0.03) compared to lamotrigine. Severe weight gain was more prevalent in non-elderly (< 65 years) than in elderly patients, with an ROR of 7.6 (p < 0.01) in those treated with lithium, and an ROR of 14.7 (p < 0.01) in those not treated with lithium.

Conclusions
Our findings suggest that lithium is associated with more reports of severe weight gain than lamotrigine, although this difference did not reach statistical significance. However, lithium use led to fewer reports of severe weight gain than some alternative drugs for long-term medication (olanzapine, quetiapine, and valproate), which is consistent with recent studies. Monitoring of weight gain and metabolic parameters remains essential with lithium and its alternatives.
Axon guidance gene-targeted siRNA delivery system improves neural stem cell transplantation therapy after spinal cord injury
Authors: Kim Seong Jun , Ko Wan-Kyu , Han Gong Ho , Lee Daye , Cho Min Jai , Sheen Seung Hun , Sohn Seil 
Published: October 2023

Abstract

Background
Neural stem cells (NSCs) derived from the embryonic spinal cord are excellent candidates for the cellular regeneration of lost neural cells after spinal cord injury (SCI). Semaphorin 3 A (Sema3A) is well known as being implicated in the major axon guidance of the growth cone as a repulsive function during the development of the central nervous system, yet its function in NSC transplantation therapy for SCI has not been investigated. Here, we report for the first time that embryonic spinal cord-derived NSCs significantly express Sema3A in the SCI environment, potentially facilitating inhibition of cell proliferation after transplantation.

Methods
siRNA-Sema3A was conjugated with poly-l-lysin-coated gold nanoparticles (AuNPs) through a charge interaction process. NSCs were isolated from embryonic spinal cords of rats. Then, the cells were embedded into a dual-degradable hydrogel with the siRNA- Sema3A loaded-AuNPs and transplanted after complete SCI in rats.

Results
The knockdown of Sema3A by delivering siRNA nanoparticles via dual-degradable hydrogels led to a significant increase in cell survival and neuronal differentiation of the transplanted NSCs after SCI. Of note, the knockdown of Sema3A increased the synaptic connectivity of transplanted NSC in the injured spinal cord. Moreover, extracellular matrix molecule and functional recovery were significantly improved in Sema3A-inhibited rats compared to those in rats with only NSCs transplanted.

Conclusions
These findings demonstrate the important role of Sema3A in NSC transplantation therapy, which may be considered as a future cell transplantation therapy for SCI cases.

Graphical Abstract