bluuebunny/crossref_metadata_2025_split huggingface dataset:
Land surface phenology and temperature variation in the International Geosphere–Biosphere Program high‐latitude transects
Authors: De Beurs Kirsten M. , Henebry Geoffrey M. 
Published: May 2005

AbstractThe International Geosphere–Biosphere Program has delineated five study areas that form a northern high‐latitude network for the analyses of vegetation and carbon dynamics. We examined the magnitude and significance of changes in the land surface phenologies of ecoregions within these transects using the NASA Pathfinder Advanced Very High‐Resolution Radiometer Land dataset. We applied the seasonal Mann–Kendall (SMK) trend test, a robust and nonparametric approach, to determine the significance of trends in the normalized difference vegetation index (NDVI) over the five transects. The SMK trend test provides an important alternative over the frequently used but unreliable trend analysis based on linear regression.In addition, we modeled the land surface phenology using quadratic or nonlinear spherical models to relate the NDVI data to accumulated growing degree‐days (base 0°C). Nonlinear spherical models parsimoniously describe the green‐up dynamics in taiga and tundra ecoregions. Models for each ecoregion within each transect were fitted separately for two time periods (1985–1988 and 1995–1999) and their parameter coefficient estimates were compared. In 10 of 24 ecoregions that comprise 72% of the land area in the transects, the date of the peak NDVI value was significantly earlier (range 2–18 days) in the second study period than in the first study period. This progression was more pronounced in North America than in Siberia (weighted average of 9.3 vs. 6.3 days earlier).Understanding of what constitutes significant change in land surface phenology amidst background variation is a critical component of global change science. A diversity of datasets, techniques, and study areas has led to a range of conclusions about boreal phenology. We discuss statistical pitfalls in standard analyses and offer a framework to conduct statistically reliable change assessments of land surface phenologies.
Losses of soil organic carbon under wind erosion in China
Authors: Yan Hao , Wang Shaoqiang , Wang Changyao , Zhang Guoping , Patel Nilanchal 
Published: May 2005

AbstractSoil organic carbon (SOC) storage generally represents the long‐term net balance of photosynthesis and total respiration in terrestrial ecosystems. However, soil erosion can affect SOC content by direct removal of soil and reduction of the surface soil depth; it also affects plant growth and soil biological activity, soil air CO2 concentration, water regimes, soil temperature, soil respiration, carbon flux to the atmosphere, and carbon deposition in soil. In arid and semi‐arid region of northern China, wind erosion caused soil degradation and desert expansion. This paper estimated the SOC loss of the surface horizon at eroded regions based on soil property and wind erosion intensity data. The SOC loss in China because of wind erosion was about 75 Tg C yr−1 in 1990s. The spatial pattern of SOC loss indicates that SOC loss of the surface horizon increases significantly with the increase of soil wind erosion intensity. The comparison of SOC loss and annual net primary productivity (NPP) of terrestrial ecosystem was discussed in wind erosion regions of China. We found that NPP is also low in the eroded regions and heavy SOC loss often occurs in regions where NPP is very small. However, there is potential to improve our study to resolve uncertainty on the soil organic matter oxidation and soil deposition processes in eroded and deposited sites.
Long‐term potential ecosystem responses to greenhouse gas‐induced thermohaline circulation collapse
Authors: Higgins Paul A. T. , Schneider Stephen H. 
Published: May 2005

AbstractAbrupt climate change, such as could occur with significant thermohaline circulation (THC) weakening, appears throughout the palaeoclimate record and in many model experiments. We examine potential responses of ecosystem structure and function to the combined influence of THC collapse and greenhouse gas increase in Central England using a broad range of temperature scenarios. We demonstrate that biological communities in the North Atlantic region could be heavily influenced by THC collapse, but that the pattern of ecosystem responses depends upon the seasonal pattern of temperature changes. Plausible THC collapse scenarios threaten the remnant habitat fragments, upon which much of England's remaining biodiversity depends, by causing shifts away from the currently dominant temperate broadleaf cold deciduous tree type. Furthermore, some ecosystem responses, particularly of energy partitioning between sensible and latent heat fluxes, constitute potentially substantial feedbacks to the local climate system. However, accurate assessment of biotic responses to THC collapse requires far better confidence of the resulting seasonal temperature cycle than climate models currently provide.
The language of ‘experience’ in nursing research
Authors: Allen David , Cloyes Kristin 
Published: May 2005

The language of ‘experience’ in nursing researchThis paper is an analysis of how the signifier ‘experience’ is used in nursing research. We identify a set of issues we believe accompany the use of experience but are rarely addressed. These issues are embedded in a spectrum that includes ontological commitments, visions of the person/self and its relation to ‘society’, understandings of research methodology and the politics of nursing. We argue that a poststructuralist understanding of the language of experience in research opens up additional ways to analyze the relationship between the conduct of nursing research and cultural/political commitments.
Modes of rationality in nursing documentation: biology, biography and the ‘voice of nursing’
Authors: Hyde Abbey , Treacy Margaret (P) , Scott P. Anne , Butler Michelle , Drennan Jonathan , Irving Kate , Byrne Anne , MacNeela Padraig , Hanrahan Marian 
Published: May 2005

Modes of rationality in nursing documentation: biology, biography, and the ‘voice of nursing’ This article is based on a discourse analysis of the complete nursing records of 45 patients, and concerns the modes of rationality that mediated text‐based accounts relating to patient care that nurses recorded. The analysis draws on the work of the critical theorist, Jürgen Habermas, who conceptualised rationality in the context of modernity according to two types: purposive rationality based on an instrumental logic, and value rationality based on ethical considerations and moral reasoning. Our analysis revealed that purposive rationality dominated the content of nursing documentation, as evidenced by a particularly bio‐centric and modernist construction of the workings of the body within the texts. There was little reference in the documentation to central themes of contemporary nursing discourses, such as notions of partnership, autonomy, and self‐determination, which are associated with value rationality. Drawing on Habermas, we argue that this nursing documentation depicted the colonisation of the sociocultural lifeworld by the bio‐technocratic system. Where nurses recorded disagreements that patients had with medical regimes, the central struggle inherent in the project of modernity became transparent — the tension between the rational and instrumental control of people through scientific regulation and the autonomy of the subject. The article concludes by problematising communicative action within the context of nursing practice.
Nursing practice as bricoleur activity: a concept explored
Authors: Gobbi Mary 
Published: May 2005

Nursing practice as bricoleur activity: a concept explored The debates concerning the nature of nursing practice are often rooted in tensions between artistic, scientific and magical/mythical practice. It is within this context that the case is argued for considering that nursing practice involves bricoleur activity. This stance, which is derived from the work of Levi‐Strauss, conceives elements of nursing practice as an embodied, bricoleur practice where practitioners draw on the ‘shards and fragments’ of the situation‐at‐hand to resolve the needs of the individual patient for whom they care. This conceptualisation of nursing practice will be analysed with a particular emphasis on its implication for nursing epistemology, pedagogy and praxis. The evidence to support this argument is drawn from empirical work that investigated nurses’ use of intuition, the work of Levi‐Strauss, and issues in nursing epistemology and ontology. The paper itself is written from the perspective of a bricoleur who uses ‘bits and pieces’ from the domains of nursing, philosophy, psychology, education, sociology and anthropology.
Phenomenology as rhetoric
Authors: Paley John 
Published: May 2005

Phenomenology as rhetoric The literature on ‘nursing phenomenology’ is driven by a range of ontological and epistemological considerations, intended to distance it from conventionally scientific approaches. However, this paper examines a series of discrepancies between phenomenological rhetoric and phenomenological practice. The rhetoric celebrates perceptions and experience; but the concluding moment of a research report almost always makes implicit claims about reality. The rhetoric insists on uniquely personal meanings; but the practice offers blank, anonymous abstractions. The rhetoric invites us to believe that knowing is subjective and involved, but at the same time it recommends a technique (bracketing) which can only represent a crude, and entirely misconceived, gesture towards objectivity. Finally, the rhetoric claims that generalisation is beside the point; but the majority of researchers generalise anyway. In quietly ignoring their own rhetoric, ‘phenomenologists’ appropriate scientific prerogatives illegitimately. For their methods do not entitle them to lay claim to anything resembling ‘objectivity’, or generalisability, or ‘reality’, or theoretical abstraction. Like other researchers, they want to talk in generalisable terms about reality; they want to be objective, they want to do theory. But they are saddled with a philosophy that is disabling, because it says they can only talk about perceptions, and meanings, and uniqueness.
Taking account of local culture: limits to the development of a professional ethos
Authors: Goopy Suzanne E. 
Published: May 2005

Taking account of local culture: limits to the development of a professional ethos The need to extend the discussion of culture in the study of nursing, combined with an enthusiasm for the possibility of viewing nursing from a new perspective, provides the impetus for this study. Based on fieldwork undertaken in the intensive care unit (RICU) of a major public hospital in Rome (Italy), this paper explores some of the key aspects of the social relations and local staff culture of one particular group of Italian nurses. In a climate of globalization, where the deployment of dominant Anglo‐American ideas is difficult to counter, the RICU presents as a setting which challenges the widespread assumptions of universal standards of nursing practice. By building a picture of the working world of these particular nurses, we are assisted in our understanding of nursing practice as a local cultural activity. In exploring the significance of local culture this paper brings into question the universality of the current paradigm of professionalism and professional identity, and emphasizes the value of acknowledging local culture.
Evidence for in situ crude oil biodegradation after the Prestige oil spill
Authors: Medina‐Bellver Javier I. , Marín Patricia , Delgado Antonio , Rodríguez‐Sánchez Alicia , Reyes Emilio , Ramos Juan L. , Marqués Silvia 
Published: May 2005

SummaryIn November 2002, the oil tanker Prestige sank off the Spanish coast after releasing approximately 17 000 tones of heavy fuel, coating several hundred kilometres of coastline in oil sludge. In December 2002 and February 2003, samples were collected from the shore of the Galician coast to analyse the indigenous population ability to carry out crude oil degradation in situ. Carbon isotopic ratio of the dissolved inorganic carbon (DIC) in seawater samples was used as a rapid method to directly assess activity of microbes on the oil components. 12CO2/13CO2 ratio in samples from certain locations along the coast revealed degradation of a very δ13C‐negative source such as the Prestige crude oil (−30.6‰). Putative biodegradation processes taking place at areas with high income of fresh seawater could not be detected with this technique. Laboratory‐scale biostimulation processes carried out in samples with the highest oil biodegradation activity showed that N/P deficiency in seawater is a limiting factor for crude oil degradation. The most probable number (MPN) of crude oil component degraders was estimated for several aromatic compounds (naphthalene, anthracene, phenanthrene, pyrene) and for undecane. Our results clearly show that bacteria present in the contaminated water are readily able to transform components of the crude oil into inorganic carbon.
Impact of lime, nitrogen and plant species on fungal community structure in grassland microcosms
Authors: Kennedy Nabla , Connolly John , Clipson Nicholas 
Published: May 2005

SummaryA microcosm‐based approach was used to study impacts of plant and chemical factors on the fungal community structure of an upland acidic grassland soil. Seven plant species typical of both unimproved and fertilized grasslands were either left unamended or treated with lime, nitrogen or lime plus nitrogen. Fungal community structure was assessed by a molecular approach, fungal automated ribosomal intergenic spacer analysis (FARISA), while fungal biomass was estimated by measuring soil ergosterol content. Addition of nitrogen (with or without lime) had the largest effect, decreasing soil pH, fungal biomass and fungal ribotype number, but there was little corresponding change in fungal community structure. Although different plant species were associated with some changes in fungal biomass, this did not result in significant differences in fungal community structure between plant species. Addition of lime alone caused no changes in fungal biomass, ribotype number or community structure. Overall, fungal community structure appeared to be more significantly affected through interactions between plant species and chemical treatments, as opposed to being directly affected by changes in individual improvement factors. These results were in contrast to those found for the bacterial communities of the same soils, which changed substantially in response to chemical (lime and nitrogen) additions.
Ecological implications of the emergence of non‐toxic subcultures from toxic Microcystis strains
Authors: Schatz D. , Keren Y. , Hadas O. , Carmeli S. , Sukenik A. , Kaplan A. 
Published: May 2005

SummaryTwo toxic, microcystin‐producing, Microcystis sp. strains KLL MG‐K and KLL MB‐K were isolated as single colonies on agar plates from Lake Kinneret, Israel. Two non‐toxic subcultures, MG‐J and MB‐J spontaneously succeeded the toxic ones under laboratory conditions. Southern analyses showed that MG‐J and MB‐J are lacking at least 34 kb of the mcy region, encoding the microcystin synthetase. Analyses of the 16S rRNA genes, the intergenic spacer region between cpcB and cpcA and the patterns of the polymerase chain reaction products of randomly amplified polymorphic DNA and highly iterated palindrome, and presence of mobile DNA elements did not allow unequivocal distinction between toxic and non‐toxic subcultures. Laboratory and field experiments indicated an advantage of the toxic strain over its non‐toxic successor. When grown separated by a membrane, which allowed passage of the media but not the cells, MG‐K severely inhibited the growth of MG‐J. Furthermore, when MG strains were placed in dialysis bags in Lake Kinneret during the season in which Microcystis is often observed, cells of MG‐J lysed, whereas MG‐K survived. Mechanisms whereby the non‐toxic subcultures emerged and prevailed over the corresponding toxic ones under laboratory conditions, as well as a possible role of microcystin under natural conditions, are discussed.
Physiological and molecular characterization of a microbial community established in unsaturated, petroleum‐contaminated soil
Authors: Kasai Yuki , Takahata Yoh , Hoaki Toshihiro , Watanabe Kazuya 
Published: May 2005

SummaryThe microbial communities established in soil samples from an unsaturated, petroleum‐contaminated zone and from an adjacent uncontaminated site were characterized by physiological and molecular approaches. Possible electron acceptors such as sulfate and nitrate had been completely depleted in these soil samples. Slurries of these soil samples were incubated in bottles in the presence of hydrocarbon indicators (benzene, toluene, xylene and decane), and the degradation of these compounds was examined. Supplementation with electron acceptors stimulated hydrocarbon degradation, although the stimulatory effect was small in the contaminated soil. The initial degradation rates in the contaminated soil under fermentative/methanogenic conditions were comparable to those under aerobic conditions. The microbial populations in the original soil samples were analysed by cloning and sequencing of polymerase chain reaction (PCR)‐amplified bacterial and archaeal 16S rRNA gene fragments, showing that the sequences retrieved from these soils were substantially different. For instance, Epsilonproteobacteria, Gammaproteobacteria, Crenarchaeota and Methanosarcinales could only be detected at significant levels in the contaminated soil. Denaturing gradient gel electrophoresis (DGGE) analyses of 16S rRNA gene fragments amplified by PCR from the incubated soil‐slurry samples showed that supplementation of the electron acceptors resulted in a shift in the major populations, while the DGGE profiles after incubating the contaminated soil under the fermentative/methanogenic conditions were not substantially changed. These results suggest that petroleum contamination of the unsaturated zone resulted in the establishment of a fermentative/methanogenic community with substantial hydrocarbon‐degrading potential.
Assemblages of ericoid mycorrhizal and other root‐associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from roots
Authors: Bougoure Damian S. , Cairney John W. G. 
Published: May 2005

SummaryEricoid mycorrhizal fungal endophytes form mycorrhizal associations with Ericaceae plant taxa and are regarded as essential to the ecological fitness of the plants in extremely nutrient‐poor soils worldwide. We isolated fungi from roots of Epacris pulchella (Ericaceae) in a south‐eastern Australian sclerophyll forest and compared rDNA internal transcribed spacer (ITS) restriction fragment length polymorphisms (RFLPs) and sequences for the cultured isolate assemblage with fungi identified in DNA extracted directly from the same root systems by cloning or denaturing gradient gel electrophoresis (DGGE). The most abundant RFLP types in the cultured isolate assemblage were identified as putative ericoid mycorrhizal ascomycete endophytes, and these also represented the most abundant RFLP types in the cloned assemblage and the most intense bands in DGGE profiles. Each method identified unique taxa, notably putative basidiomycetes in the DNA extracted directly from E. pulchella roots. However, the relative abundance of these was low.
Stable isotope probing analysis of the influence of liming on root exudate utilization by soil microorganisms
Authors: Rangel‐Castro J. Ignacio , Killham Ken , Ostle Nick , Nicol Graeme W. , Anderson Ian C. , Scrimgeour Charlie M. , Ineson Phil , Meharg Andy , Prosser Jim I. 
Published: May 2005

SummaryRhizosphere microorganisms play an important role in soil carbon flow, through turnover of root exudates, but there is little information on which organisms are actively involved or on the influence of environmental conditions on active communities. In this study, a 13CO2 pulse labelling field experiment was performed in an upland grassland soil, followed by RNA‐stable isotope probing (SIP) analysis, to determine the effect of liming on the structure of the rhizosphere microbial community metabolizing root exudates. The lower limit of detection for SIP was determined in soil samples inoculated with a range of concentrations of 13C‐labelled Pseudomonas fluorescens and was found to lie between 105 and 106 cells per gram of soil. The technique was capable of detecting microbial communities actively assimilating root exudates derived from recent photo‐assimilate in the field. Denaturing gradient gel electrophoresis (DGGE) profiles of bacteria, archaea and fungi derived from fractions obtained from caesium trifluoroacetate (CsTFA) density gradient ultracentrifugation indicated that active communities in limed soils were more complex than those in unlimed soils and were more active in utilization of recently exuded 13C compounds. In limed soils, the majority of the community detected by standard RNA‐DGGE analysis appeared to be utilizing root exudates. In unlimed soils, DGGE profiles from 12C and 13C RNA fractions differed, suggesting that a proportion of the active community was utilizing other sources of organic carbon. These differences may reflect differences in the amount of root exudation under the different conditions.
Moderately thermophilic Myxobacteria: novel potential for the production of natural products isolation and characterization
Authors: Gerth Klaus , Müller Rolf 
Published: May 2005

SummaryNovel moderately thermophilic Myxobacteria from both known suborders (Cystobacterineae and Sorangineae) were isolated from soil samples of semiarid and warm climates. The addition of the anthelmintic and amoebizidal agent levamisole was a new and crucial improvement for the fast isolation of overall 37 strains, which grew very fast at temperatures of 42–44°C. When the 16S rDNAs were compared with GenBank data of common Myxobacteria, identities were 98–99%, thus not reflecting the physiological differences. Similar to the Myxobacteria described so far, the new isolates are multiresistant against a variety of antibiotics and are producers of typical myxobacterial secondary metabolites. Analysis of our previous strain collection isolated from soil samples taken worldwide revealed a more or less uniform distribution of strains which synthesize specific metabolites. Therefore these moderately thermophilic Myxobacteria, which grow 2–3 times faster, have the potential to replace the slow‐growing isolates and provide a means for fast and cost‐saving production of myxobacterial metabolites in the future.
Persistence of vibrios in marine bivalves: the role of interactions with haemolymph components
Authors: Pruzzo Carla , Gallo Gabriella , Canesi Laura 
Published: May 2005

SummaryMarine bivalves are widespread in coastal environments and, due to their filter‐feeding habit, they can accumulate large numbers of bacteria thus acting as passive carriers of human pathogens. Bivalves possess both humoral and cellular defence mechanisms that operate in a co‐ordinated way to kill and eliminate infecting bacteria. Vibrio species are very abundant in coastal waters and are commonly isolated from edible bivalves tissues where they can persist after depuration processes in controlled waters. Such observations indicate that vibrios are regular components of bivalve microflora and that the molluscs can represent an important ecological niche for these bacteria. Here we tried to summarize data on the interactions between vibrios and bivalve haemolymph; the available evidence supports the hypothesis that persistence of bacteria in bivalve tissues depends, at least in part, on their sensitivity to the bactericidal activity of the haemolymph. Results obtained with an in vitro model of Vibrio cholerae challenged against Mytilus galloprovincialis haemocytes indicate that bacterial surface components, soluble haemolymph factors and the signalling pathways of the haemocyte host are involved in determining the result of vibrio–haemolymph interactions.