bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the Catalytic Efficiency of Supported NHC‐Ag(I) Complexes in the Borono‐Minisci Reaction
Authors: Moroni Giada , Bombonato Elena , Bonafè Samuele , Di Michele Alessandro , Presenti Sara , Guariento Sara , Marcaccio Massimo , Sardella Roccaldo , Ronchi Paolo , Gioiello Antimo 
Published: February 2024

AbstractA series of supported N‐heterocyclic carbene silver complexes (NHC‐Ag(I)) were prepared and characterized as catalysts for the borono‐Minisci reaction. After characterization, the synthesized catalysts were evaluated in batch mode to determine the reaction performance and kinetics. Interestingly, cyclic voltammetric analysis showed that the structure of both the complex and the ligand significantly influences the Ag(I)/Ag(II) redox potential and, in turn, the catalytic efficiency. Among the tested catalysts, the Si‐supported NHC‐Ag(I) 6 afforded the desired products in good to excellent yields in only 15 min, providing a complementary tactic to standard homogeneous approaches.
Highly Efficient Blue Fluorescent Organic Light‐Emitting Devices Based on λ5‐Phosphinine Derivatives
Authors: Sugiyama Ryo , Okada Riku , Noda Taito , Meguro Naoki , Yoshida Naoto , Hoshi Keigo , Ohta Hidetoshi , Hayashi Minoru , Sasabe Hisahiro , Kido Junji 
Published: February 2024

AbstractAlthough λ5‐phosphinine derivatives are known as a promising class of blue fluorescent emitters, those photoluminescent quantum yield (PLQY) values have been reached up to 92 %, however, only a few examples have been explored as an emitter for blue organic light‐emitting device (OLED), and the external quantum efficiency (EQE) has been below 2.4 % so far. In this study, we newly developed two types of blue λ5‐phosphinine derivatives namely CN‐COCF3 and CO2Me‐CHO, and investigated the photophysical properties in the solid states. The photophysical analyses in solid state films suggested that the strong electron‐accepting nature of these λ5‐phosphinine derivatives caused the inferior PLQY values, and the exciplex formation with the host and neighboring materials should be avoided to improve the device efficiency. By choosing suitable host and neighboring materials with deep ionization potentials, we successfully realized efficient blue fluorescent OLEDs with EQE of over 4 % and CIE (0.14, 0.18). This is among the best in λ5‐phosphinine‐based blue OLEDs so far.
Rapid on‐site evaluation of touch imprint cytology in navigation bronchoscopy for small peripheral pulmonary nodules
Authors: Kops Stephan E. P. , van der Burgt Lizanne J. W. , Vos Shoko , van Zuijlen‐Manders Lia J. M. , Verhoeven Roel L. J. , van der Heijden Erik H. F. M. 
Published: February 2024

AbstractBackgroundRapid on‐site evaluation (ROSE) of cytopathology plays an important role in determining whether representative samples have been taken during navigation bronchoscopy. With touch imprint cytology (TIC), histologic samples can be assessed using ROSE. Although advised by guidelines, there have been almost no studies on the performance of TIC during navigation bronchoscopy. The objective of this study was to evaluate the value of TIC‐ROSE (forceps/cryobiopsy) in combination with conventional ROSE (cytology needle/brush).MethodsIn this single‐center, prospective cohort study, patients who had pulmonary nodules with an indication for navigation bronchoscopy were consecutively included. The primary outcome of the study was the concordance of ROSE and the procedural outcome. The concordance rates of TIC‐ROSE and the combination of TIC‐ROSE plus conventional ROSE were compared.ResultsFifty‐eight patients with 66 nodules were included. Conventional ROSE and TIC‐ROSE were assessable in 61 nodules (90.9%) each. By combining both ROSE techniques, all sampled lesions were assessable. Combining conventional ROSE with TIC‐ROSE showed concordant results in 51 of 66 cases (77.3%) versus 44 of 66 (66.7%) and 48 of 66 (72.8%) concordant results for conventional ROSE and TIC‐ROSE alone, respectively, compared with the procedural outcome. There was no indication of tissue depletion as a result of TIC. The combined ROSE approach had a statistically significant higher concordance rate compared with conventional ROSE alone.ConclusionsTIC‐ROSE is a cheap, easily implementable technique that can result in higher concordant ROSE outcomes. This could lead to more efficient procedures and possibly higher diagnostic results. In a monomodality sampling setting with only histologic samples, TIC can provide ROSE.
Distribution of gamma‐aminobutyric acid immunoreactivity in the brain of the Siberian sturgeon (Acipenser baeri): Comparison with other fishes
Authors: Anadón Ramón , Rodríguez‐Moldes Isabel , Adrio Fátima 
Published: February 2024

AbstractGamma‐aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the central nervous system (CNS) of vertebrates. Immunohistochemical techniques with specific antibodies against GABA or against its synthesizing enzyme, glutamic acid decarboxylase (GAD) allowed characterizing GABAergic neurons and fibers in the CNS. However, studies on the CNS distribution of GABAergic neurons and fibers of bony fishes are scant and were done in teleost species. With the aim of understanding the early evolution of this system in bony vertebrates, we analyzed the distribution of GABA‐immunoreactive (‐ir) and GAD‐ir neurons and fibers in the CNS of a basal ray‐finned fish, the Siberian sturgeon (Chondrostei, Acipenseriformes), using immunohistochemical techniques. Our results revealed the presence and distribution of GABA/GAD‐ir cells in different regions of the CNS such as olfactory bulbs, pallium and subpallium, hypothalamus, thalamus, pretectum, optic tectum, tegmentum, cerebellum, central grey, octavolateralis area, vagal lobe, rhombencephalic reticular areas, and the spinal cord. Abundant GABAergic innervation was observed in most brain regions, and GABAergic fibers were very abundant in the hypothalamic floor along the hypothalamo‐hypophyseal tract and neurohypophysis. In addition, GABA‐ir cerebrospinal fluid‐contacting cells were observed in the alar and basal hypothalamus, saccus vasculosus, and spinal cord central canal. The distribution of GABAergic systems in the sturgeon brain shows numerous similarities to that observed in lampreys, but also to those of teleosts and tetrapods.
Amphiphilic Chlorin‐β‐cyclodextrin Conjugates in Photo‐Triggered Drug Delivery: The Role of Aggregation
Authors: Panagiotakis Stylianos , Mavroidi Barbara , Athanasopoulos Alexandros , Charalambidis Georgios , Coutsolelos Athanassios G. , Pelecanou Maria , Yannakopoulou Konstantina 
Published: February 2024

AbstractConjugates of chlorins with β‐cyclodextrin connected either directly or via a flexible linker were prepared. In aqueous medium these amphiphilic conjugates were photostable, produced singlet oxygen at a rate similar to clinically used temoporfin and formed irregular nanoparticles via aggregation. Successful loading with the chemotherapeutic drug tamoxifen was evidenced in solution by the UV‐Vis spectral changes and dynamic light scattering profiles. Incubation of MCF‐7 cells with the conjugates revealed intense spotted intracellular fluorescence suggestive of accumulation in endosome/lysosome compartments, and no dark toxicity. Incubation with the tamoxifen‐loaded conjugates revealed also practically no dark toxicity. Irradiation of cells incubated with empty conjugates at 640 nm and 4.18 J/cm2 light fluence caused >50 % cell viability reduction. Irradiation following incubation with tamoxifen‐loaded conjugates resulted in even higher toxicity (74 %) indicating that the produced reactive oxygen species had triggered tamoxifen release in a photochemical internalization (PCI) mechanism. The chlorin‐β‐cyclodextrin conjugates displayed less‐lasting effects with time, compared to the corresponding porphyrin‐β‐cyclodextrin conjugates, possibly due to lower tamoxifen loading of their aggregates and/or their less effective lodging in the cell compartments’ membranes. The results suggest that further to favorable photophysical properties, other parameters are important for the in vitro effectiveness of the photodynamic systems.
Photoinduced [2+2] and [4+4] Cycloaddition and Cycloreversion Reactions for the Development of Photocontrollable DNA Binders
Authors: Dohmen Christoph , Ihmels Heiko 
Published: February 2024

AbstractIn the current field of photopharmacology, molecular photoswitches are applied whose interactions with DNA can be triggered or controlled by light. And although several photochromic reactions have been shown to serve this purpose well, the reversible photocycloaddition and photocycloreversion reactions have been largely neglected. This absence of research is surprising because especially the photodimerization of a DNA ligand leads to products with significant change of the size and shape which, in turn, leads to strongly diminished or even suppressed DNA association. Therefore, photocycloaddition–cycloreversion sequences have a huge potential for the photoinduced, reversible deactivation and activation of ligand–DNA interactions, as will be shown with selected examples in this Concept Article. Specifically, heterostyryl and ‐stilbene derivatives are presented whose DNA–binding properties are efficiently switched in reversible [2+2] photocycloaddition reactions. In addition, the photocontrolled DNA–binding of anthracene derivatives and their heterocyclic benzo[b]quinolizinium analogues in a [4+4] photocycloaddition, as well as the use of this reaction as part of dual–mode switches in combination with redox‐active functionalities, are highlighted. Furthermore, examples of conjugates are provided, in which the photochromic unit is bound covalently to nucleic acids or proteins, such that the photocycloaddition reaction can be used for reversible photoinduced crosslinking, ligation, or inhibition of gene expression.
Attendant, autonomous, audacious, and advisory boards: Catalyzing corporate environmental performance
Authors: del Villegas‐Periñán María del Mar , Barroso‐Castro Carmen , Villalba‐Ríos Paula , Vecino‐Gravel Julio Darío 
Published: February 2024

AbstractBoards of directors play important roles when responding to one of the biggest challenges for companies today: environmental sustainability. Research has mainly been focused on the separate impacts of board structure, composition, and processes on corporate environmental performance. Understanding how these three dimensions of the board, which help to successfully fulfill the board roles, interact in complementary or substitutive ways is crucial for improving companies' environmental performance. Using a fuzzy‐set qualitative comparative analysis on a sample of 86 European‐listed companies in greenhouse gas (GHG)‐intensive sectors, our study shows that board dimensions and the attributes that shape them have complementary or substitutable effects on corporate environmental performance. Specifically, we identified four board archetypes that arise from different configurations promoting environmental performance. Finally, we considered CEO‐Board interaction, showing that both longer and shorter CEO tenures together with certain combinations of board attributes could be linked to higher environmental performance.
Enhancing HLA‐B27 antigen detection: Leveraging machine learning algorithms for flow cytometric analysis
Authors: Baráth Sándor , Singh Parvind , Hevessy Zsuzsanna , Ujfalusi Anikó , Mezei Zoltán , Balogh Mária , Száraz Széles Marianna , Kappelmayer János 
Published: February 2024

AbstractAs the association of human leukocyte antigen B27 (HLA‐B27) with spondylarthropathies is widely known, HLA‐B27 antigen expression is frequently identified using flow cytometric or other techniques. Because of the possibility of cross‐reaction with off target antigens, such as HLA‐B7, each flow cytometric technique applies a “gray zone” reserved for equivocal findings. Our aim was to use machine learning (ML) methods to classify such equivocal data as positive or negative. Equivocal samples (n = 99) were selected from samples submitted to our institution for clinical evaluation by HLA‐B27 antigen testing. Samples were analyzed by flow cytometry and polymerase chain reaction. Features of histograms generated by flow cytometry were used to train and validate ML methods for classification as logistic regression (LR), decision tree (DT), random forest (RF) and light gradient boost method (GBM). All evaluated ML algorithms performed well, with high accuracy, sensitivity, specificity, as well as negative and positive predictive values. Although, gradient boost approaches are proposed as high performance methods; nevertheless, their effectiveness may be lower for smaller sample sizes. On our relatively smaller sample set, the random forest algorithm performed best (AUC: 0.92), but there was no statistically significant difference between the ML algorithms used. AUC values for light GBM, DT, and LR were 0.88, 0.89, 0.89, respectively. Implementing these algorithms into the process of HLA‐B27 testing can reduce the number of uncertain, false negative or false positive cases, especially in laboratories where no genetic testing is available.
Changes in affect longitudinally mediate associations between emotion regulation strategy use and disordered eating
Authors: Mikhail Megan E. , Burt S. Alexandra , Neale Michael C. , Keel Pamela K. , Katzman Debra K. , Klump Kelly L. 
Published: February 2024

AbstractBackgroundTrait‐level emotion regulation (ER) difficulties are associated with eating disorders (EDs) transdiagnostically. However, little research has examined whether within‐person fluctuations in ER longitudinally predict ED behaviors in daily life or the mechanisms of ER effects. Investigating daily ER could help us better understand why people experience ED behaviors at a given time. We examined whether day‐to‐day changes in adaptive (e.g., cognitive reappraisal) and maladaptive (e.g., rumination) ER longitudinally predicted core ED behaviors (binge eating, purging, dieting) and whether changes in affect mediated effects.MethodFemale participants (N = 688) ages 15–30 from the Michigan State University Twin Registry reported their adaptive and maladaptive ER use, negative affect (NA), positive affect (PA), binge eating, purging, and dieting on 49 consecutive days. Using structural equation modeling, we examined whether within‐person fluctuations in ER predicted same‐ and next‐day ED behaviors and whether changes in affect mediated longitudinal ER effects.ResultsGreater maladaptive ER predicted increased likelihood of same‐day binge eating and next‐day binge eating and purging. The association between maladaptive ER and next‐day binge eating and purging was mediated by increased next‐day NA. In contrast, dieting was more closely related to changes in PA. Adaptive ER did not predict reduced likelihood of any ED behavior.ConclusionsMaladaptive ER may longitudinally increase risk for binge eating and purging by amplifying NA. Interventions focused on decreasing maladaptive ER and subsequent NA might help disrupt binge eating‐purging cycles. Conversely, results add to evidence that PA fluctuations may play a unique role in maintaining restrictive behaviors.Public SignificanceLittle is known about how daily changes in emotion regulation may impact disordered eating. We found that maladaptive emotion regulation (e.g., rumination) was associated with a higher likelihood of binge eating and purging on the next day because it predicted increased next‐day negative affect. In contrast, dieting was more closely tied to fluctuations in positive affect. Targeting daily emotion regulation and affective processes may help disrupt cycles of disordered eating.
Serum metabonomic study to explore the amelioration mechanisms of co‐fermented collagen peptide‐jackfruit juice on the immunosuppressed mice
Authors: Ma Tingting , Cheng Ming , Wen Xin , Zhu Kexue , Zhang Xin , Zhao Chao , El‐Seedi Hesham R. , Lin Xiangdong , Li Yongcheng , Zhu Lulu , Cao Jun , Li Chuan 
Published: February 2024

AbstractThe main purpose of this study was to investigate the improvement effect and serum metabolomic analysis of co‐fermented collagen peptide‐jackfruit juice (FPJ) against immunosuppression in cyclophosphamide‐induced mice. Serum samples were analyzed using ultra‐performance liquid chromatography coupled with mass spectrometry (UPLC‐MS/MS) targeted metabolomics technology, including the normal group, model group, and FPJ group, and determine if such treatments were able to revert immunosuppression‐related metabolic changes to normal status. The results showed that 32 differential metabolites were upregulated and 42 metabolites were downregulated in the model group compared with the normal group. After FPJ intervention, there was a trend of normal callback. Adenosine, inosine, guanosine, 2‐deoxyadenosine, hypoxanthine, xanthine,
d‐xylulose‐5‐phosphate, ribulose‐5‐phosphate, phenylpyruvic acid, hippuric acid, 3‐(3‐hydroxyphenyl)‐propionic acid, 3‐ureidopropionate, uridine, dCDP and malonic acid may be used as predictive markers of immunosuppression. Notably, these biomarkers were involved in purine metabolism, pentose phosphate pathway, phenylalanine metabolism, and pyrimidine metabolism. In this study, FPJ inhibited oxidative stress and improved the gut microbes by regulating the disorders of metabolic pathways. The metabolomics approach developed herein provides novel insight into the metabolic disturbances associated with immunosuppression, which will contribute to understanding the mechanism of FPJ on immunosuppression further and providing a basis for further research.
Ligand Functionalization of Metal‐Organic Frameworks for Photocatalytic H2O2 Production
Authors: Qiu Jianhao , Yao Jianfeng 
Published: February 2024

AbstractH2O2 production by photocatalysis has received considerable attention, while using MOF‐based materials as photocatalysts is still less explored. Ligand functionalization of MOFs is a decoration way at the molecular level, which provides the basis for the further modification of MOFs. In this concept, ligand functionalization of MOFs for photocatalytic H2O2 production has been systematically and extensively discussed. The strategies of ligand functionalization include grafting of functional groups (e. g. amino, methoxyl, boron and alkyl) and multiple ligand decoration. Correspondingly, some specific effects are achieved, e. g. improved charge carrier separation, enhanced O2 adsorption and hydrophobicity, which can realize efficient H2O2 production by photocatalysis. Meanwhile, reaction conditions and activities of photocatalytic H2O2 production have also been explicitly introduced in these studies. At last, the future perspectives are proposed. This concept sheds fresh light on the great potential of ligand functionalization of MOFs for photocatalytic H2O2 production.
A preliminary study characterizing temporal changes in soil bacterial communities after dismembered bones were buried
Authors: Wang Jiaqi , Liu Zidong , Ren Jianbo , Zhang Mingming , Guan Zimeng , Zhao Xingchun , Gao Cairong , Zhang Gengqian 
Published: February 2024

AbstractDetermining the burial time of skeletal remains is one of the most important issues of forensic medicine. We speculated that the microbiome of gravesoil may be a promising method to infer burial time by virtue of time‐dependent. As we know, forensic scientists have established various models to predict the postmortem interval of a decedent based on the changes in body and soil microbiome communities. However, limited data are available on the burial time prediction for bones, especially dismembered bones. In this exploratory study, we initially conducted 16S rRNA amplicon high‐throughput sequencing on the burial soil of 10 porcine femurs within a 120‐day period and analyzed the changes in soil microbial communities. Compared with the control soil, a higher Shannon index in the microbial diversity of burial soil containing bones was observed. Correlation analysis identified 61 time‐related bacterial families and the best subset selection method obtained best subset, containing Thermomonosporaceae, Clostridiaceae, 0319‐A21, and Oxalobacteraceae, which were used to construct a simplified multiple linear regression model with a mean absolute error (MAE) of 56.69 accumulated degree day (ADD). An additional random forest model was established based on indicators for the minimum cross‐validation error of Thermomonosporaceae, Clostridiaceae, 0319‐A21, Oxalobacteraceae, and Syntrophobacteraceae, with an MAE of 55.65 ADD. The produced empirical data in this pilot study provided the evidence of feasibility that the microbial successional changes of burial soil will predict the burial time of dismembered bones and may also expand the current knowledge of the effects of bone burial on soil bacterial communities.
Comparative analysis of micronucleus induction and DNA damage biomarkers in TK6 and A375 cells using flow cytometry
Authors: Sun Xiaowen , Spellman Richard A. , Engel Maria , Rubitski Elizabeth , Schuler Maik 
Published: February 2024

AbstractPreviously, we introduced an alternative adherent A375 cell line for clastogenicity and aneugenicity testing using a high content imaging platform. To further characterize the performance of A375 cells, we investigated the sensitivity and specificity of A375 and TK6 cells by directly comparing micronucleus (MN) induction, cytotoxicity (relative cell counts, viability, and apoptosis), clastogenicity (γH2AX), and aneuploidy markers (pH 3, MPM‐2, and polyploidy) using flow cytometric methods. We evaluated 14 compounds across different mechanisms (non‐genotoxic apoptosis inducers, clastogens, and aneugens with either tubulin binding or aurora kinase inhibiting phenotypes) at 4‐h and 24‐h post treatment. Both aneugens and clastogens tested positive for micronucleus induction in both cell lines. Apoptosis continued to be a confounding factor for flow cytometry‐based micronuclei assessment in TK6 cells as evidenced by positive responses by the three cytotoxicants. Conversely, A375 cells were not affected by apoptosis‐related false positive signals and did not produce a positive response in the in vitro micronucleus assay. Benchmark dose response (BMD) analysis showed that the induction of micronuclei and biomarkers occurred at similar concentrations in both cell lines for clastogens and aneugens. By showing that A375 cells have similar sensitivity to TK6 cells but a greater specificity, these results provide additional support for A375 cells to be used as an alternative adherent cell line for in vitro genetic toxicology assessment.