bluuebunny/crossref_metadata_2025_split huggingface dataset:
Challenges experienced by cardiac intensive care nurses during first out‐of‐bed patient mobilization after open‐heart surgery: A descriptive phenomenological qualitative study
Authors: Ünver Seher , Yildirim Meltem , Akbal Sevim , Sever Seda 
Published: February 2024

AbstractAimTo investigate the potential challenges experienced by cardiac intensive care unit (ICU) nurses in the first out‐of‐bed patient mobilization after open‐heart surgery and propose solutions.DesignA qualitative study design using the descriptive phenomenological approach.MethodsIndividual face‐to‐face interviews were conducted via a widely used videoconferencing program between 28 July and 22 October 2022, in the tertiary cardiac ICU of a university hospital. Nurses who had at least 1 year of cardiac ICU experience and actively participating in patient care were included. The COREQ criteria and checklist were followed in this investigation and the data were analysed by using the ATLAS.ti 8.0.FindingsNurses reported that they experience challenges when mobilizing patients, such as patients' fear, reluctance or resistance; nurses having insufficient experience, strength or lack of team members. Despite these challenges, they highlighted some positive outcomes of mobilization, such as feeling happy, reinforced team communication and nurse–patient relationship. The nurses also suggested some facilitators, such as patient motivation and an explanation of the process.ConclusionNurses experience various patient‐related and nurse‐related challenges during the first out‐of‐bed mobilization of the patient after open‐heart surgery. It is recommended that healthcare institutions should implement effective strategies to address the staffing shortages and to support nurse motivation to ensure adequate nursing care.ImpactThis study provides valuable insights into the existing literature by examining the potential challenges and strategies of the first out‐of‐bed patient mobilization in the cardiac ICU. It shows that patient education and organizational adaptations are effective ways to overcome the challenges. It also suggests that motivating and informing patients before the mobilization can make the process easier. Moreover, it reveals that successful patient mobilization makes nurses happy, improves team communication and strengthens nurse–patient relationship.Patient and Public Involvement and EngagementNo patient or public contribution.
A strengths‐based qualitative exploration of critical care nurses' reasons for remaining in critical care
Authors: Phillips Emily K. , Dunsford Jennifer , Van Haute Stephanie , Throndson Karen , Schultz Annette S. H. 
Published: February 2024

AbstractAimsGlobally, the nursing shortage is a growing concern. Much of the research on retention of nurses focuses on the experience of those who left positions. In this study, we set out to listen to critical care nurses (CCRNs) who have chosen to remain in their positions to understand the factors retaining them in critical care.DesignThis interpretive descriptive study was guided by the following research question: ‘what factors influence CCRN's decision to continue to work in critical care?’MethodsDigitally recorded interviews and a focus group were conducted between July 2022 and January 2023 using a semi‐structured, strengths‐based interview guide with CCRNs from three critical care units at a tertiary hospital in a city in a central Canadian province. Transcribed interviews were analysed using open, axial and selective coding and constant comparative analysis.ResultsTwenty‐two CCRNs participated in interviews and three in a focus group. The theme of Respect, demonstrated through the interconnected concepts of Working to Full Scope, Team, Rotations and Compensation was identified. Working to Full Scope was described as providing nursing care aligned with how each nurse envisions what nursing is. Being part of a Team led by strong nurse leaders that provides opportunities and supports the sharing of their perspectives was also found. Respect was also found to be demonstrated through Rotations that recognize that work is one part of these nurses' lives. Compensation that reflects the increased education, knowledge and skills required in critical care was the final concept of Respect.ConclusionOrganizations should focus their efforts across the identified concepts to demonstrate Respect for CCRNs and retain them.Implications for PracticeThe findings of this study provide ways to support the retention of CCRNs.ImpactThis research will have an impact on nursing leaders by providing tangible ways to retain CCRNs.Reporting MethodReporting of this work was guided by the Standards for Reporting Qualitative Research.Patient or Public ContributionNo patient or public contribution.
Evaluation of patients with implantable cardioverter‐defibrillator in a Latin American tertiary center
Authors: França Anna Terra , Martins Larissa Natany Almeida , de Oliveira Derick Matheus , de Castilho Fábio Morato , Branco Beatriz Castello , Wilnes Bruno , Ribeiro Antônio Luiz P. , Carmo André Assis Lopes do 
Published: February 2024

AbstractIntroductionDespite advancements in implantable cardioverter‐defibrillator (ICD) technology, sudden cardiac death (SCD) remains a persistent public health concern. Chagas disease (ChD), prevalent in Brazil, is associated with increased ventricular tachycardia (VT) and ventricular fibrillation (VF) events and SCD compared to other cardiomyopathies.MethodsThis retrospective observational study included patients who received ICDs between October 2007 and December 2018. The study aims to assess whether mortality and VT/VF events decreased in patients who received ICDs during different time periods (2007–2010, 2011–2014, and 2015–2018). Additionally, it seeks to compare the prognosis of ChD patients with non‐ChD patients. Time periods were chosen based on the establishment of the Arrhythmia Service in 2011. The primary outcome was overall mortality, assessed across the entire sample and the three periods. Secondary outcomes included VT/VF events and the combined outcome of death or VT/VF.ResultsOf the 885 patients included, 31% had ChD. Among them, 28% died, 14% had VT/VF events, and 37% experienced death and/or VT/VF. Analysis revealed that period 3 (2015–2018) was associated with better death‐free survival (p = .007). ChD was the only variable associated with a higher rate of VT/VF events (p < .001) and the combined outcome (p = .009).ConclusionMortality and combined outcome rates decreased gradually for ICD patients during the periods 2011–2014 and 2015–2018 compared to the initial period (2007–2010). ChD was associated with higher VT/VF events in ICD patients, only in the first two periods.
Association between HDL‐C and intensive blood pressure control in patients with hypertension: A post‐hoc analysis of SPRINT
Authors: Liu Rufei , Cheng Wenli 
Published: February 2024

AbstractPrevious studies in patients with hypertension have demonstrated that there is a U‐shaped association between HDL‐C (high‐density lipoprotein cholesterol) and the risk of cardiovascular events in male patients with hypertension. However, to the best of our knowledge, the relationship between HDL‐C and intensive blood pressure control in specific cardiovascular events has never been investigated. To fill this knowledge gap, the authors analyzed the relationship between HDL‐C levels and cardiovascular events in hypertensive patients within the Systolic Blood Pressure Intervention Trial (SPRINT). The SPRINT evaluated the impact of intensive blood pressure control (systolic blood pressure < 120 mm Hg) versus standard blood pressure control (systolic blood pressure < 140 mm Hg). The Cox proportional risk regression was used to investigate the association between different HDL‐C status and clinical outcomes. Additional stratified analyzes were performed to evaluate the robustness of sex difference. A total of 9323 participants (6016 [64.53%] males and 3307 [35.47%] females) with hypertension from the SPRINT research were included in the analysis. The median follow‐up period was 3.26 years. Our population was divided into five groups based on the HDL‐C plasma levels: HDL‐C < 30 mg/dL, HDL‐C between 30 and 40 mg/dL, HDL‐C between 40 and 60 mg/dL, HDL‐C between 60 and 80 mg/dL and HDL‐C > 80 mg/dL. Sensitivity analyzes showed that in the SPRINT, women in the HDL‐C high population had a higher risk of mortality from all causes than men. In this cohort study, results suggest that patients with HDL‐C levels higher than 80 mg/dL had lower risk of SPRINT primary outcome, cardiovascular death, and stroke, but this study tested association, not causation. HDL‐C levels were associated with composite cardiovascular outcomes in male but not female patients. Our results demonstrated that in patients with hypertension, the association between HDL‐C and risk of cardiovascular events is L‐shaped.
Investigation of the effect of virtual reality distraction in patients undergoing mandibular periodontal surgery: A randomized controlled study
Authors: Gurbuz Ezgi , Gurbuz Ali Aycan 
Published: February 2024

AbstractObjectiveThis clinical study aimed to evaluate the effect of virtual reality (VR) technology on anxiety and pain levels in patients undergoing gingivectomy and gingivoplasty procedures.Materials and MethodsThe patients were randomized into test (surgery with VR glasses) and control (surgery without VR glasses) groups. Before the surgery, the anxiety level was measured using the modified dental anxiety scale (MDAS), and anticipated pain (AP) was measured by a visual analog scale (VAS). Immediately after the surgery, the pain and discomfort associated with the surgery (VASP), procedure time (T), and time perception (TP) were evaluated. The patients in the test group were requested to assess the immersion (VASI), satisfaction (VASS), perception of reduced anxiety (VASA), and perceived control (VASC). One week after surgery, MDAS was applied to all patients.ResultsThis trial was conducted with 41 female and 17 male subjects with a mean age of 29.69 ± 12.32. There were no significant differences between the groups in terms of age, sex, preoperative MDAS, or AP. After surgery, MDAS, VASP, T, and TP failed to differ significantly between the groups. The subject age was positively correlated with VASI, VASS, VASA, and VASC (r = 0.60, p = 0.00; r = 0.44, p = 0.02; r = 0.46, p = 0.02; r = 0.50, p = 0.01, respectively) and negatively correlated with VASP (r = 0.47, p = 0.04).ConclusionsVR application did not affect anxiety and pain levels in patients undergoing periodontal surgery. More studies are needed to evaluate VR distraction in periodontal surgeries with diverse age samples and video options.Clinical SignificanceVR does not affect anxiety and pain levels during gingivectomy and gingivoplasty surgeries in the young adult population. It should be evaluated in older age groups. Trial registration ClinicalTrials.gov Identifier: NCT06092177.
The BTB/TAZ domain‐containing protein CmBT1‐mediated CmANR1 ubiquitination negatively regulates root development in chrysanthemum
Authors: Du Lian‐Da , Guan Zhang‐Ji , Liu Yan‐Hong , Zhu Hui‐Dong , Sun Quan , Hu Da‐Gang , Sun Cui‐Hui 
Published: February 2024

ABSTRACTRoots are fundamental for plants to adapt to variable environmental conditions. The development of a robust root system is orchestrated by numerous genetic determinants and, among them, the MADS‐box gene ANR1 has garnered substantial attention. Prior research has demonstrated that, in chrysanthemum, CmANR1 positively regulates root system development. Nevertheless, the upstream regulators involved in the CmANR1‐mediated regulation of root development remain unidentified. In this study, we successfully identified bric‐a‐brac, tramtrack and broad (BTB) and transcription adapter putative zinc finger (TAZ) domain protein CmBT1 as the interacting partner of CmANR1 through a yeast‐two‐hybrid (Y2H) screening library. Furthermore, we validated this physical interaction through bimolecular fluorescence complementation and pull‐down assays. Functional assays revealed that CmBT1 exerted a negative influence on root development in chrysanthemum. In both in vitro and in vivo assays, it was evident that CmBT1 mediated the ubiquitination of CmANR1 through the ubiquitin/26S proteasome pathway. This ubiquitination subsequently led to the degradation of the CmANR1 protein and a reduction in the transcription of CmANR1‐targeted gene CmPIN2, which was crucial for root development in chrysanthemum. Genetic analysis suggested that CmBT1 modulated root development, at least in part, by regulating the level of CmANR1 protein. Collectively, these findings shed new light on the regulatory role of CmBT1 in degrading CmANR1 through ubiquitination, thereby repressing the expression of its targeted gene and inhibiting root development in chrysanthemum.
How to make a digital reconstruction of the human ribcage
Authors: López‐Rey José M. , García‐Martínez Daniel , Bastir Markus 
Published: February 2024

AbstractUp to now, there have been no publication standardizing the digital reconstruction of the modern human ribcage from commingled costo‐vertebral material. Consequently, we designed a validated protocol based on anatomical features observed in the literature and the CT scanned ribcages of 10 adult European individuals. After quantifying the shape of these ribcages using 3D geometric morphometrics, we split each vertebra and rib within their corresponding (semi)landmarks. Subsequently, individual bones + (semi)landmarks were imported to LhpFusionBox, commingled and 3D reconstructed. To validate the accuracy of the protocol, we first reconstructed a randomly chosen ribcage three times and then compared these reconstructions to the rest of the sample. Since these reconstructions were closer to their original counterpart than to the others, the remaining sample was reconstructed once. Next, we tested the intra‐observer error during reconstructing using the Procrustes distances among the original ribcages and the reconstructions. We observed that first each ribcage reconstruction was clustered to its original counterpart and second there was a learning curve showing an improvement in the reconstruction process over time. Subsequently, we explored general size and shape differences among the original and reconstructed ribcages through a study of centroid size and a permutation test on the Procrustes distances (10,000 permutations), respectively. Specific shape differences between both groups were further examined through a principal component analysis in shape space. None of these analyses found statistical differences between the original and reconstructed ribcages (p > 0.05). Eventually, we extracted the mean shapes of the original ribcages and the reconstructions in order to visualize potential deviations caused by the anatomical considerations of the researcher. These results demonstrate that the protocol is accurate enough to be used when reconstructing a disarticulated human ribcage.
HHLA2 is more significantly associated with poor prognosis in TSCC than PD‐L1
Authors: Li Yi , Yang Qiong , Yang Xu , Ge Jie , Xu Tianshu , Liu HuaLian 
Published: February 2024

AbstractBackgroundThe incidence and mortality of tongue squamous cell carcinoma have shown an alarming increase in recent years. This study aimed to investigate the potential of HHLA2 as an immune checkpoint in comparison to PD‐L1.MethodsWe obtained RNA‐seq data from TCGA to study HHLA2 and PD‐L1 expression across various tissues. Using the CIBERSORT package, we estimated cell type abundances within mixed populations based on gene expression profiles. Immunohistochemistry was performed to analyze HHLA2 and PD‐L1 expression in Tongue squamous cell carcinoma. Prognostic evaluation was carried out with Kaplan–Meier curves and the log‐rank test. To explore factors affecting HHLA2, univariate and multivariate Cox regression analyses were conducted with the COX regression model. Additionally, we used single‐cell RNA sequencing data from the GEO database for gene set enrichment analysis with genes strongly correlated with HHLA2.ResultsOur analysis of RNA‐seq data unveiled a significant upregulation of HHLA2 and PD‐L1 expression in primary tumors when compared with normal tissue. HHLA2 exhibited a positive expression rate of 36.9%, while PD‐L1 had a positive expression rate of 24.6%. HHLA2 emerged as a noteworthy independent risk factor impacting the overall survival of Tongue squamous cell carcinoma patients. The analysis of scRNA‐seq data shed light on the involvement of HHLA2 in key pathways related to cell cycle regulation and interferon alpha/beta signaling.ConclusionsThis study suggests that in the context of Tongue squamous cell carcinoma, HHLA2 may represent a more promising target for immunotherapy when compared with PD‐L1.
Abnormal temporal variability of thalamo‐cortical circuit in patients with moderate‐to‐severe obstructive sleep apnea
Authors: Long Ting , Shu Yongqiang , Liu Xiang , Huang Ling , Zeng Li , Li Lifeng , Zhan Jie , Li Haijun , Peng Dechang 
Published: February 2024

SummaryThis study investigated the abnormal dynamic functional connectivity (dFC) variability of the thalamo‐cortical circuit in patients with obstructive sleep apnea (OSA) and explored the relationship between these changes and the clinical characteristics of patients with OSA. A total of 91 newly diagnosed patients with moderate‐to‐severe OSA and 84 education‐matched healthy controls (HCs) were included. All participants underwent neuropsychological testing and a functional magnetic resonance imaging scan. We explored the thalamo‐cortical dFC changes by dividing the thalamus into 16 subregions and combining them using a sliding‐window approach. Correlation analysis assessed the relationship between dFC variability and clinical features, and the support vector machine method was used for classification. The OSA group exhibited increased dFC variability between the thalamic subregions and extensive cortical areas, compared with the HCs group. Decreased dFC variability was observed in some frontal‐occipital‐temporal cortical regions. These dFC changes positively correlated with daytime sleepiness, disease severity, and cognitive scores. Altered dFC variability contributed to the discrimination between patients with OSA and HCs, with a classification accuracy of 77.8%. Our findings show thalamo‐cortical overactivation and disconnection in patients with OSA, disrupting information flow within the brain networks. These results enhance understanding of the temporal variability of thalamo‐cortical circuits in patients with OSA.
Influence of local environmental gradients on ecological strategies of herbaceous communities in riverine side channels
Authors: Gaudichet Corentin , Greulich Sabine , Grellier Séraphine , Janssen Philippe , Rodrigues Stéphane 
Published: February 2024

AbstractQuestionsIn riverine side channels (secondary and former arms), the alternation of lotic, lentic and dry phases produces disturbance and resource gradients that filter species and community strategies. To better understand the impact of this alternation of functioning on plant communities at a fine spatial scale, we tested for the effects of elevation (linked to water level), slope, longitudinal position, light availability and sediment grain size on (i) taxonomic composition and (ii) competition, stress tolerance, and ruderal strategies (C, S and R strategies) at the community level. We hypothesized that fewer resources would increase the stress‐tolerant prevalence (i.e., community‐weighted mean value), while topographic gradients would affect ruderal prevalence. In addition, we hypothesized that strong and directional environmental filtering should reduce the co‐occurrence of species with different CSR strategy values (i.e., low C, S and R functional dispersion).LocationFourteen side channels on the lower reach of the Loire river (France).MethodsTaxonomic composition of the herb layer and environmental variables were assessed on 474 plots. The relations between species composition and environmental gradients were investigated by a constrained analysis of principal coordinates. The effects of environmental gradients on community CSR strategies were assessed using mixed‐effects models and a model‐averaging procedure.ResultsTaxonomic community composition was shaped mainly by light availability, topographic elevation and sediment grain size. Slope and the interaction between light and elevation influenced the community‐weighted mean and functional dispersion values of S and R strategies. Among other interactions, those involving sediment grain size shaped the dispersion values for C and R strategies.ConclusionThe study identified shading and fine sediment grain size as key drivers of biotic homogenization in the riverscape. Increasing the disturbance frequency and magnitude would limit the progression of the ecological succession and favor the co‐occurrence of taxonomically and functionally diverse communities within the side channels.
Red meat intake, faecal microbiome, serum trimethylamine N‐oxide and hepatic steatosis among Chinese adults
Authors: Huang Yong , Zhang Jiawei , Zhang Yaozong , Wang Wuqi , Li Meiling , Chen Bo , Zhang Xiaoyu , Zhang Zhuang , Huang Jiaqi , Jin Yong , Wang Hua , Zhang Xuehong , Yin Shi , Yang Wanshui 
Published: February 2024

AbstractBackground and AimsEmerging evidence suggests a detrimental impact of high red meat intake on hepatic steatosis. We investigated the potential interplay between red meat intake and gut microbiome on circulating levels of trimethylamine N‐oxide (TMAO) and hepatic steatosis risk.MethodsThis cross‐sectional study was conducted in a representative sample of 754 community‐dwelling adults in Huoshan, China. Diet was collected using 4 quarterly 3 consecutive 24‐h dietary (12‐day) recalls. We profiled faecal microbiome using 16S ribosomal RNA sequencing and quantified serum TMAO and its precursors using LC‐tandem MS (n = 333). We detected hepatic steatosis by FibroScan. The adjusted odds ratios (aORs) and 95% confidence intervals (CIs) were calculated using logistic regression.ResultsTMAO levels but not its precursors were positively associated with the likelihood of hepatic steatosis (aOR per 1‐SD increment 1.86, 95% CI 1.04–3.32). We identified 14 bacterial genera whose abundance was associated with TMAO concentration (pFDR < .05) belonging to the phyla Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria families. Per 10 g/day increase in red meat intake was positively associated with TMAO levels among participants who had higher red meat intake (>70 g/day) and higher TMAO‐predicting microbial scores (TMS, β = .045, p = .034), but not among others (pinteraction = .030). TMS significantly modified the positive association between red meat and steatosis (pinteraction = .032), with a stronger association being observed among participants with higher TMS (aOR 1.30, 95% CI 1.07–1.57).ConclusionsThe bacterial genera that predicted TMAO levels may jointly modify the association between red meat intake and TMAO levels and the subsequent risk of hepatic steatosis.