bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects and Mechanisms of Fucoxanthin from Hizikia fusiforme on Inhibiting Tongue Squamous Cell Carcinoma Proliferation via AKT/mTOR-Mediated Glycolysis
Authors: Zhang Xiu Qiang , Liu Tao Yu , Zhang Lu Tao , Hua Zheng Hao , Jin Xuan An , Xu Feng , Ji Jin Chao , Xu Bo Huai , Ding Hao Miao 
Published: November 2023

Fucoxanthin, a natural carotenoid, contains special ene bonds and 5, 6-mono-epoxide compounds in its molecule, which has physiological activities such as anticancer, antioxidant, anti-inflammatory, antiobesity, and antidiabetic. However, the anticancer actions and underlying mechanisms of fucoxanthin on oral cancer remain to be assessed. In this study, we used tongue carcinoma (CAL-27) cells to examine the effects and underlying mechanisms of fucoxanthin derived from Hizikia fusiforme on CAL-27 proliferation. MTT assays were used to estimate fucoxanthin’s effect on the proliferative capacity of CAL-27 cells. Flow cytometry was used to examine how fucoxanthin affects apoptosis initiation and alters cell cycle progression in CAL-27 cells. Spectrophotometry was used to assess the impact of fucoxanthin on adenosine triphosphate (ATP) generation, glucose uptake, lactate production, and the enzymatic activities of pyruvate kinase and hexokinase in CAL-27 cells. Western blotting was used to investigate fucoxanthin’s impact on the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway and glycolysis-related protein expression levels in CAL-27 cells. Fucoxanthin significantly inhibited CAL-27 cell viability time- and dose-dependently

p

<

0.01

. Administration of fucoxanthin induced CAL-27 cell apoptosis and cell cycle arrest at the G1 phase

p

<

0.05

. Furthermore, fucoxanthin significantly decreased glucose uptake, lactate production, and ATP production in CAL-27 cells

p

<

0.01

and significantly inhibited glycolysis-related enzyme activities and protein expression levels

p

<

0.05

. Fucoxanthin also substantially decreased the expression levels of phosphorylated ribosomal protein S6, phosphorylated AKT, and phosphorylated mTOR

p

<

0.05

. In conclusion, fucoxanthin inhibited CAL-27 cell proliferation, and this mechanism may be associated with the AKT/mTOR-mediated glycolysis pathway.
PRMT1 Inhibition Activates the Interferon Pathway to Potentiate Antitumor Immunity and Enhance Checkpoint Blockade Efficacy in Melanoma
Authors: Tao Hongru , Jin Chen , Zhou Liyuan , Deng Zhenzhong , Li Xiao , Dang Wenzhen , Fan Shijie , Li Bing , Ye Fei , Lu Junyan , Kong Xiangqian , Liu Chuanpeng , Luo Cheng , Zhang Yuanyuan 
Published: November 2023

Abstract

Despite the immense success of immune checkpoint blockade (ICB) in cancer treatment, many tumors, including melanoma, exhibit innate or adaptive resistance. Tumor-intrinsic T-cell deficiency and T-cell dysfunction have been identified as essential factors in the emergence of ICB resistance. Here, we found that protein arginine methyltransferase 1 (PRMT1) expression was inversely correlated with the number and activity of CD8+ T cells within melanoma specimen. PRMT1 deficiency or inhibition with DCPT1061 significantly restrained refractory melanoma growth and increased intratumoral CD8+ T cells in vivo. Moreover, PRMT1 deletion in melanoma cells facilitated formation of double-stranded RNA derived from endogenous retroviral elements (ERV) and stimulated an intracellular interferon response. Mechanistically, PRMT1 deficiency repressed the expression of DNA methyltransferase 1 (DNMT1) by attenuating modification of H4R3me2a and H3K27ac at enhancer regions of Dnmt1, and DNMT1 downregulation consequently activated ERV transcription and the interferon signaling. Importantly, PRMT1 inhibition with DCPT1061 synergized with PD-1 blockade to suppress tumor progression and increase the proportion of CD8+ T cells as well as IFNγ+CD8+ T cells in vivo. Together, these results reveal an unrecognized role and mechanism of PRMT1 in regulating antitumor T-cell immunity, suggesting PRMT1 inhibition as a potent strategy to increase the efficacy of ICB.

Significance:
Targeting PRMT1 stimulates interferon signaling by increasing expression of endogenous retroviral elements and double-stranded RNA through repression of DNMT1, which induces antitumor immunity and synergizes with immunotherapy to suppress tumor progression.
SUMOylation-Driven mRNA Circularization Enhances Translation and Promotes Lymphatic Metastasis of Bladder Cancer
Authors: Zhao Yue , Chen Jiancheng , Zheng Hanhao , Luo Yuming , An Mingjie , Lin Yan , Pang Mingrui , Li Yuanlong , Kong Yao , He Wang , Lin Tianxin , Chen Changhao 
Published: November 2023

Abstract

Aberrant gene expression is a prominent feature of metastatic cancer. Translational initiation is a vital step in fine-tuning gene expression. Thus, exploring translation initiation regulators may identify therapeutic targets for preventing and treating metastasis. Herein, we identified that DHCR24 was overexpressed in lymph node (LN) metastatic bladder cancer and correlated with poor prognosis of patients. DHCR24 promoted lymphangiogenesis and LN metastasis of bladder cancer in vitro and in vivo. Mechanistically, DHCR24 mediated and recognized the SUMO2 modification at lysine 108 of hnRNPA2B1 to foster TBK1 mRNA circularization and eIF4F initiation complex assembly by enhancing hnRNPA2B1–eIF4G1 interaction. Moreover, DHCR24 directly anchored to TBK1 mRNA 3′-untranslated region to increase its stability, thus forming a feed forward loop to elevate TBK1 expression. TBK1 activated PI3K/Akt signaling to promote VEGFC secretion, resulting in lymphangiogenesis and LN metastasis. DHCR24 silencing significantly impeded bladder cancer lymphangiogenesis and lymphatic metastasis in a patient-derived xenograft model. Collectively, these findings elucidate DHCR24-mediated translation machinery that promotes lymphatic metastasis of bladder cancer and supports the potential application of DHCR24-targeted therapy for LN-metastatic bladder cancer.

Significance:
DHCR24 is a SUMOylation regulator that controls translation initiation complex assembly and orchestrates TBK1 mRNA circularization to activate Akt/VEGFC signaling, which stimulates lymphangiogenesis and promotes lymph node metastasis in bladder cancer.
Insect galls of Parque Nacional da Serra dos Órgãos (Rio de Janeiro State, Southeastern Brazil)
Authors: Maia Valéria Cid , Mascarenhas Bernardo 
Published: November 2023

The Parque Nacional da Serra dos Órgãos (PARNASO) is an integral protection conservation unit inserted in the domain of the Atlantic forest. This Park was investigated monthly from September to December/2021 and from March to May/2022 for insect galls. Host plants were identified and their exsiccates were deposited in the herbarium (R) of the Museu Nacional. Insects obtained by gall dissection and rearing were deposited in the Entomological Collection of Museu Nacional (MNRJ). The conservational status of the host plants and their origin were verified in the site Flora e Funga do Brasil (2023). New records of host plants in Brazil are presented based on comparison with literature data. PARNASO hosts a great richness of galls (290 gall morphotypes on 43 plant families). Myrtaceae, Melastomataceae, Asteraceae, Fabaceae, and Rubiaceae, Mikania Willd. (Asteraceae), Miconia Ruiz & Pav. (Melastomataceae), and Eugenia L. (Myrtaceae), and Guapira opposita (Nyctaginaceae) were the botanical taxa with the highest number of gall morphotypes, all of them were previously indicated as super hosts in inventories in forest formations of the Atlantic forest, except Rubiaceae. PARNASO includes 148 gall-inducing species native to Brazil, being 69 endemic; among the last 23 are exclusive to the Atlantic forest. Eight gall-inducing species are endangered and four are near threathned. Most galls were found on leaves. The most frequent gall characters were: globoid shape, green color, and glabrous surface. Most galls were induced by Cecidomyiidae (Diptera), however galls of Lepidoptera, Coleoptera, Hemiptera, Thysanoptera, and Hymenoptera wera also found. These are the known patterns in Brazil. The secondary fauna was reported in 62 gall morphotypes and included parasitoids (Hymenoptera), successors (Collembola, Hemiptera and Thysanoptera), cecidophagous (Lepidoptera), and predators (spiders). Parasitoids were the most frequent. Seven gall midge species are recorded for the first time in this Park.
Evaluating functional brain organization in individuals and identifying contributions to network overlap
Authors: Bijsterbosch Janine D. , Farahibozorg Seyedeh-Rezvan , Glasser Matthew F. , Van Essen David , Snyder Lawrence H. , Woolrich Mark W. , Smith Stephen M. 
Published: November 2023

Abstract
Individual differences in the spatial organization of resting-state networks have received increased attention in recent years. Measures of individual-specific spatial organization of brain networks and overlapping network organization have been linked to important behavioral and clinical traits and are therefore potential biomarker targets for personalized psychiatry approaches. To better understand individual-specific spatial brain organization, this paper addressed three key goals. First, we determined whether it is possible to reliably estimate weighted (non-binarized) resting-state network maps using data from only a single individual, while also maintaining maximum spatial correspondence across individuals. Second, we determined the degree of spatial overlap between distinct networks, using test-retest and twin data. Third, we systematically tested multiple hypotheses (spatial mixing, temporal switching, and coupling) as candidate explanations for why networks overlap spatially. To estimate weighted network organization, we adopt the Probabilistic Functional Modes (PROFUMO) algorithm, which implements a Bayesian framework with hemodynamic and connectivity priors to supplement optimization for spatial sparsity/independence. Our findings showed that replicable individual-specific estimates of weighted resting-state networks can be derived using high-quality fMRI data within individual subjects. Network organization estimates using only data from each individual subject closely resembled group-informed network estimates (which was not explicitly modeled in our individual-specific analyses), suggesting that cross-subject correspondence was largely maintained. Furthermore, our results confirmed the presence of spatial overlap in network organization, which was replicable across sessions within individuals and in monozygotic twin pairs. Intriguingly, our findings provide evidence that overlap between 2-network pairs is indicative of coupling. These results suggest that regions of network overlap concurrently process information from both contributing networks, potentially pointing to the role of overlapping network organization in the integration of information across multiple brain systems.
‘I haven’t got a clue!’
Authors: Fendel Victoria Beatrix 
Published: November 2023

Abstract
Support-verb constructions are combinations of a verb and a noun that act as the predicate, as ‘made the suggestion’ in I made the suggestion that she join. They are frequent, variable, and ambiguous across texts, as well as language-specific in their lexical and syntactic properties. The article examines patterns of negation with δίκην δίδωμι ‘to pay the price for one’s actions’, ὅπλα ἔχω ‘to be armed’, and συμμαχίαν ποιέομαι ‘to ally up’ in classical literary Attic. Syntactically and lexically, support-verb constructions can behave like a word or like a syntagm. A word does not have an internal syntax but only an external one; a syntagm has an internal syntax. Negation in support-verb constructions can be achieved either morpho-syntactically or lexically as long as the syntagm character has not faded. Morpho-syntactically, support-verb constructions can be negated by drawing on their external or their internal syntax. Lexical negation can be achieved by means of negative verbs of realisation indicating a zero-degree of multiplication. If available for a support-verb construction, lexical negation appears to add a nuance of intensity; morpho-syntactic negation appears primarily in contrastive contexts.
ECtHR Case-law Concerning Russian Aggression on Ukraine and the Events Taking Place after 2014
Authors: Czepek Jakub J. 
Published: November 2023

Abstract
Ukraine has faced ongoing armed conflict within the eastern parts of its territory since 2014. The state witnessed the annexation of Crimea, de facto occupation of Donetsk and Lugansk regions, the shooting down of Flight MH-17, and numerous human rights violations in the eastern parts of the country. Since the Russian aggression in 2022, Ukraine has faced armed conflict throughout the country. At the same time, Russia and Ukraine had been states parties to the European Convention on Human Rights (ECHR). Russia ceased to be a party to the ECHR on 16 September 2022, due to its expulsion from the Council of Europe (CoE) six months earlier. All the applications against the Russian Federation filed to the European Court on Human Rights (ECtHR) before this date should – and will – be examined by the Court. This research mainly aims to analyse the Court’s existing case-law concerning the events in Ukraine after 2014, and the ECtHR jurisprudence concerning armed conflicts. The purpose of such analysis is to examine the possibilities and challenges the Court will face in its forthcoming judgments in inter-state applications filed by Ukraine against Russia. It should be stressed that the execution of these future judgments may also be an issue.
Lactobacillus probiotics restore vaginal and gut microbiota of pregnant women with vaginal candidiasis
Authors: Ang X.-Y. , Roslan N.S. , Ahmad N. , Yusof S.Mo. , Abdullah N. , Nik Ab Rahman N.N. , Woon J.-J. , Teh C.S.-J. , Todorov S.D. , Liu G. , Liong M.-T. 
Published: November 2023

Abstract
The development of probiotics has now included the areas along the gut-vaginal axis. We thus aimed to investigate the effects of lactobacilli probiotic to modulate and restore vaginal and gut microbiota of pregnant women with vaginal candidiasis (VC). A randomised, double-blind and placebo-controlled study was performed in 78 pregnant women with VC. Patients were randomised to either the probiotic (SynForU-HerCare) or placebo which were administered at baseline and continued for 8-weeks (two capsules/day of 9.5 log cfu/capsule). Microbiota profiles were assessed at time points of weeks-0, 4 and 8 for high vaginal swab and faecal samples. Shannon diversity index showed that after 8-weeks amid VC, a shift in microbial community compositional changes occurred in the high vaginal region at both genus () and species () levels, where the administration of probiotic prevented such a shift. These changes were mainly attributed to a decreased in abundance of Lactobacillus () accompanied by increased abundance of Prevotella () and Atopobium () in the placebo group while the probiotic group remained unchanged over time. The administration of probiotics also prevented a reduced abundance of faecal phylum Firmicutes after 8-weeks as seen in the placebo group (), which also showed reduction at subsequent taxonomic levels of class, family, genera and species. VC has not only altered the microbiota of vagina regions but also gut microbiota profiles, causing lessening of gut microbiota that are crucial for gut nutrient availability, protection and immunity. The administration of lactobacilli probiotics has prevented such a shift, leading to better modulated gut and vaginal microenvironment amid VC.
The study was registered at ClinicalTrials.gov: identifier number NCT03940612
Empty or non-empty site? Evidence from the acquisition of Najdi Arabic sluicing
Authors: Alaowffi Nouf 
Published: November 2023

Abstract
Sluicing—a clausal ellipsis in which the only pronounced part of a question is the wh-phrase—has been controversial since its emergence (Ross, 1969). I address herein the existence and nature of the ellipsis site’s content with an experimental argument from the field of first language acquisition. Using intervention effects as a diagnostic of movement, this study examines whether Najdi Arabic (NA)-speaking children show a subject advantage in sluicing and whether a mismatch in animacy features aids their interpretation of object sluices. A yes/no question task was conducted with 48 NA-speaking children. The results revealed that NA-speaking children exhibit a statistically significant subject advantage, although economy considerations constrain when this subject advantage emerges. Yet, no effect of mismatching animacy was found. Regardless, the results argue in favour of the structural-movement analysis of sluicing. This study contributes to the persistent debate on ellipsis site content by providing an experimental argument that relies on data from the acquisition of sluicing in NA.
Historical sound changes in Central Chadic (Afroasiatic)
Authors: Wolff H. Ekkehard 
Published: November 2023

Abstract
In its entirety as much as with regard to its four branches, the Chadic language family poses challenges to the application of the Neogrammarian-school comparative method, not the least because of the immense time-depth involved and aggravated by certain typological peculiarities of Chadic phonology and morphology as inherited from its Afroasiatic ancestry. This is particularly true for Central Chadic, which—with 80 languages—is the most numerous and most diverse branch of Chadic, which in total counts almost 200 languages and thereby more than half of all known Afroasiatic languages. Both more or less ‘regular’ and ‘sporadic’ sound changes criss-cross the territories currently occupied by speakers of Central Chadic languages in Nigeria, Cameroon, and Chad, which allow confident identification of cognates. Some cognates have survived many millennia of language history practically unchanged with regard to their phonetic realisations, while others differ remarkably from the reconstructed proto-language forms beyond ad hoc recognisability. Recent historical comparative research by Richard Gravina (2014, 2015) and the present author (2022, 2023) involving 66 spoken languages and some 230 cognate lexical items have unearthed much of the linguistic histories behind the massive synchronic diversity of the modern Central Chadic languages. The recent research by the author has added to our understanding of historical Central Chadic phonology by unravelling the phonological processes by which the languages have developed sets of new phonemes, in addition to tracing more or less ‘regular’ and ‘sporadic’ sound changes that the PCC segmental inventories of vowels and consonants underwent. Modern Central Chadic languages show sets of innovative vowels and consonant whose emergence can largely be attributed, besides occasional instances of segmental fusion, to the ‘colouring’ effects of so-called prosodies. This makes the analysis of prosodic features such as palatalisation, labialisation, prenasalisation and glottalisation essential in order to understand the evolution of modern Central Chadic languages.
Establishing Shintō Funerals in Edo Japan
Authors: Tan Tiantian 
Published: November 2023

Abstract
In early modern Japan, the Buddhist monopoly on death-related rituals eventually triggered a movement for the development of Shintō funerals. Based on an analysis of ritual texts produced by several Shintō groups—Yoshida Shintō, Yoshikawa Shintō, Suika Shintō, and Kokugaku Shintō—this study delineates how each group adopted and localized Confucian discourses to establish Shintō funerals during the Edo period. While there were Buddhist overtones in the practices of Yoshida Shintō, clergy from the other three groups frequently borrowed from Confucian rituals in their construction of Shintō-style funerals. Attempting to revive ancient Japanese funerary rites, scholars created Shintō versions of Confucian-style spirit tablets and justified Confucian rituals by invoking the practices of the kami. This study provides a deeper understanding of the history of Shintō funerals, and the inherent Shintōization of Confucian practices therein, while also exploring the relationship between Buddhist, Confucian, and Shintō thought in Edo-period Japan.
The Nobel and the Price of Prestige in the 21st Century
Authors: English James F. 
Published: November 2023

Abstract
In the late 20th century, the Nobel Prize enjoyed an unrivaled position as the most powerful instrument of literary consecration. But recent shifts in the relationship between money and prestige, and in the forms of scandal that attend cultural awards, are weakening the Nobel’s symbolic hold. Symptomatic missteps by the Swedish Academy, occurring against a backdrop of increasingly extreme concentration of wealth, have opened a window of opportunity for the founding of a new super-prize better suited than the Nobel to dominate today’s economy of world-literary prestige.
Forging Bonds across Continents: Italian Merchants and Īl-Khānid Diplomacy
Authors: Liberati Riccardo 
Published: November 2023

Abstract
This article analyses the Italian commercial presence in the Mongol Īl-Khānate in thirteenth-century Persia. Analysing source materials, the study focuses on the experiences of individuals and communities alike, showcasing a dual aspiration to economic gain and political status. The study examines mechanisms that facilitated merchants’ relationship with the Īl-Khānids, leading Italians to occupy significant positions at the Īl-Khānid court. It also explains how just a few individuals were instrumental in fostering diplomatic ties with Europe and enabling treaties that bolstered Genoese and Venetian communities in Tabriz and beyond. A subsequent phase marked a shift as Īl-Khānid rulers embraced Islam, causing relations with Europe to erode, thereby diminishing Italian influence. This intricate interplay between Italian merchants’ trade, diplomatic endeavours, and cultural exchanges highlights the multifaceted nature of historical interactions in this period.
Distinct Features of Atmospheric Rivers over the Western North Pacific in Post–El Niño and Non-Post–El Niño Summers
Authors: Guo Xiaojun , Zhao Ning , Nasuno Tomoe , Kikuchi Kazuyoshi , Nakano Masuo , Annamalai H. 
Published: November 2023

Abstract
An atmospheric river (AR) is a highly accumulated line of moisture, which often preconditions heavy rainfall events. Considering the short-lived nature of ARs, we aim to understand their characteristics over the western North Pacific (WNP) in boreal summer, focusing on the difference between the post–El Niño (pEN) and non-post–El Niño (npEN) cases in weekly segments rather than the seasonal mean. We found that during AR-active weeks in pEN summers, a meridional dipole pattern of anomalous geopotential height with a more westward-extended WNP subtropical high dominates, favoring AR activity over southern China to the central Pacific. In npEN summers, in contrast, a zonal dipole pattern with an anomalous low over southeast China and an anomalous high over subtropical WNP is pronounced during the AR-active weeks, which is accompanied with warm sea surface temperature anomalies and greater downward shortwave radiation to the southeast of Japan. Such anomalous circulation leads to enhanced AR occurrence over the northern South China Sea (SCS) to Japan. The above differences in the AR features between the two cases appear as the distinct regionality in the AR-related rainfall over eastern China and Japan. Temporal decompositions reveal that both seasonal mean and submonthly variations play dominant roles in the anomalous circulation during AR-active weeks in pEN summers, while the submonthly variations are more pronounced in npEN summers. Further analysis also suggests that tropical cyclones could impact AR occurrence in both cases with different patterns of atmospheric responses, inducing remarkable AR enhancement over the northern SCS in npEN summers.