bluuebunny/crossref_metadata_2025_split huggingface dataset:
EEG functional connectivity in infants at elevated familial likelihood for autism spectrum disorder
Authors: O’Reilly Christian , Huberty Scott , van Noordt Stefon , Desjardins James , Wright Nicky , Scorah Julie , Webb Sara Jane , Elsabbagh Mayada ,   BASIS team, Baron-Cohen Simon , Bolton Patrick , Chandler Susie , Charman Tony , Fernandes Janice , Garwood Holly , Hudryx Kristelle , Johnson Mark H. , Tucker Leslie , Volein Agnes 
Published: October 2023

Abstract
Background
Many studies have reported that autism spectrum disorder (ASD) is associated with atypical structural and functional connectivity. However, we know relatively little about the development of these differences in infancy.

Methods
We used a high-density electroencephalogram (EEG) dataset pooled from two independent infant sibling cohorts, to characterize such neurodevelopmental deviations during the first years of life. EEG was recorded at 6 and 12 months of age in infants at typical (N = 92) or elevated likelihood for ASD (N = 90), determined by the presence of an older sibling with ASD. We computed the functional connectivity between cortical sources of EEG during video watching using the corrected imaginary part of phase-locking values.

Results
Our main analysis found no significant association between functional connectivity and ASD, showing only significant effects for age, sex, age-sex interaction, and site. Given these null results, we performed an exploratory analysis and observed, at 12 months, a negative correlation between functional connectivity and ADOS calibrated severity scores for restrictive and repetitive behaviors (RRB).

Limitations
The small sample of ASD participants inherent to sibling studies limits diagnostic group comparisons. Also, results from our secondary exploratory analysis should be considered only as potential relationships to further explore, given their increased vulnerability to false positives.

Conclusions
These results are inconclusive concerning an association between EEG functional connectivity and ASD in infancy. Exploratory analyses provided preliminary support for a relationship between RRB and functional connectivity specifically, but these preliminary observations need corroboration on larger samples.
Low pressure headache and cerebral fat embolism from a sacral fracture through a Tarlov cyst: a case report
Authors: Anandkumar Delran , Jakhura Umayr , Potter Kathleen , Bhatti Ibrahim 
Published: October 2023

Abstract
Background
Here we report the only formally documented case in the United Kingdom, to our knowledge, of a cerebral fat embolism secondary to non-iatrogenic trauma through a Tarlov cyst. This case demonstrates the pathology clearly giving an excellent opportunity to demonstrate a rarely seen pathology as well as illustrating the importance of the patient history to guiding further management.

Case presentation
A middle-aged patient was admitted on the acute medical take complaining of severe headache with photophobia, having just returned after a skiing holiday. Computerised tomography scan of the head showed fat within the anterior horn of both lateral ventricles, and within the subarachnoid space. Re-discussion with the patient and subsequent MRI (Magnetic Resonance Imaging) of the spine identified the pathogenesis of her symptoms: a sacral insufficiency fracture through a Tarlov cyst, causing subarachnoid fat embolism and symptoms of a low-pressure headaches due to a dural leak. Patient was medically managed and discharged with planned follow-up. Due to the Coronavirus pandemic and resolution of the patient's symptoms, they declined further follow up imaging.

Conclusions
The case demonstrates a rarely seen pathology as cause of a common presenting problem, headache. Emphasizing the importance of history taking and appropriate investigations in medical cases that do not conform to the usual diagnosis.
Diversity when interpreting evidence in network meta-analyses (NMAs) on similar topics: an example case of NMAs on diabetic macular oedema
Authors: Wu Jing , Adams Clive , He Xiaoning , Qi Fang , Xia Jun 
Published: October 2023

Abstract
Background
Different network meta-analyses (NMAs) on the same topic result in differences in findings. In this review, we investigated NMAs comparing aflibercept with ranibizumab for diabetic macular oedema (DME) in the hope of illuminating why the differences in findings occurred.

Methods
Studies were searched for in English and Chinese electronic databases (PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang, VIP; see detailed search strategy in the main body). Two independent reviewers systematically screened to identify target NMAs that included a comparison of aflibercept and ranibizumab in patients with DME. The key outcome of interest in this review is the change in best-corrected visual acuity (BCVA), including various ways of reporting (such as the proportion of participants who gain ≥ 10 ETDRS letters at 12 months; average change in BCVA at 12 months).

Results
For the binary outcome of BCVA, different NMAs all agreed that there is no clear difference between the two treatments, while continuous outcomes all favour aflibercept over ranibizumab. We discussed four points of particular concern that are illustrated by five similar NMAs, including network differences, PICO (participants, interventions, comparators, outcomes) differences, different data from the same measures of effect, and differences in what is truly significant.

Conclusions
A closer inspection of each of these trials shows how the methods, including the searches and analyses, all differ, but the findings, although presented differently and sometimes interpreted differently, were similar.
Determination of causes of adult deaths using minimally invasive tissue sampling in Gandaki province of Nepal: a multicenter hospital-based study
Authors: Subedi Nuwadatta , Bhattarai Suraj , Ranabhat Sunita , Sharma Binita Koirala , Baral Madan Prasad 
Published: October 2023

Abstract
Background
Minimally Invasive Tissue Sampling (MITS) has been successfully used to establish the cause of death in low- and middle-income countries, mostly in stillbirths and neonates. The objective of this study was to determine the causes of death among adults using MITS in the Gandaki province of Nepal and to find out the contribution of MITS to identify the causes of death.

Methods
A multicentric hospital-based pilot study was conducted to enroll 100 cases of adult deaths. The specimens of cerebrospinal fluid, blood, brain, lungs, and liver tissue were collected utilizing MITS. These specimens underwent standard histopathological, serological, and microbiological analyses. The findings from MITS, and if available, clinical records and forensic autopsy findings were compiled and the cause of death panel identified the causes of death. The final cause of death allocated to each case was based on the WHO International Medical Certificate of Death.

Results
Among a total of 100 cases enrolled during the study period, infectious cause attributed to the immediate cause of death in 77 (77%), cardiovascular in 10 (10%), neurological in 8 (8%), malignancy in two (2%), and gastrointestinal and hepatobiliary cause in one (1%) case. The mean age of the cases was 50.8 ± 15.9 years and 76 (76%) were males. MITS established the cause of death in the causal chain of events in 81(81%) cases and identified the cause of death significantly more with infectious than non-infectious causes (p < 0.001).

Conclusions
MITS was useful in establishing the cause of death in the majority of adult deaths and the most common cause was infectious disease. Our findings suggest that MITS can be a valuable and alternative tool for mortality surveillance in low-resource settings, where complete diagnostic autopsies are less accepted or less prioritized.
Analysis of the relationship between the temporal and spatial evolution of henan grotto temples and their geographical and cultural environment based on GIS
Authors: Li Yu 
Published: October 2023

Abstract
Grotto temples in Henan Province, which began in the Northern Wei Dynasty and ended in the Republic of China, have been excavated and repaired to varying degrees in the Sui, Tang, Song, Yuan, Ming and Qing dynasties. In this study, 119 grotto temples sited in Henan Province were considered as the research object, and the spatial and temporal distribution of grotto temples in Henan Province is analyzed by using the method of quantitative geographic analysis and ArcGIS 10.5 software. The analysis results indicate that the grrotto temples (including cliff statues) in Henan Province tend to have a cohesive distribution, with uneven regional distribution, mainly concentrated in the western and northern regions of Henan. The distribution direction and center of the grotto temples (including cliff statues) did not change much during the Northern Dynasties, Sui and Tang dynasties, and Song and Yuan dynasties, mainly distributing in the northwest of Henan. However, the situation underwent significant changes and began to migrate to south of Henan, reflecting the changes the changes in the political and cultural center at that time. The spatiotemporal distribution of the cave temples (including cliff statues) was based on the related topography, river systems, stratigraphic lithology, road traffic, and was direct or indirect influenced by the authorities as well.
Synergy and regulation of the South China Karst WH site integrity protection and the buffer zone agroforestry development
Authors: Xiong Kangning , Chen Dong , Zhang Juan , Gu Xinyan , Zhang Ning 
Published: October 2023

Abstract
The Natural World Heritage site (NWH) has a dual role of protection and utilization. The buffer zone of the South China Karst (SCK) World Heritage (WH) site is scattered with village dwellings, which adds pressure to preserve the outstanding universal value (OUV) and integrity protection of the WH site. The development of agroforestry is an essential means to maintain rural livelihoods, protect the ecological environment, and realize the protection and development of the heritage site. Studying the synergy and regulation of the heritage site integrity protection and the buffer zone agroforestry development is essential. This study takes the Shibing and Libo–Huanjiang karst WH as the study areas and administrative villages as the evaluation unit. From 2020 to 2023, through data methods such as remote sensing data interpretation, information data survey and collection, comprehensive index analysis, coupling coordination degree model, and GIS spatial analysis, a systematic study was conducted on the basic frontier research, synergy relationship, and regulation of the heritage site integrity protection and the buffer zone agroforestry development. The results demonstrate that the Shibing and Libo–Huanjiang karst WH sites integrity protection and agroforestry development in the buffer zone have a high coupling degree and a good coupling coordination relationship. This reveals that the heritage site integrity protection and the buffer zone agroforestry development are an interconnected and mutually influential whole, which the synergistic relationship between them is developing in a positive direction; The buffer zone agroforestry development lags behind the heritage site integrity protection, based on which proposed the regulatory measures for the synergy development of the heritage site integrity protection and agroforestry development in the buffer zone. The findings in this study provide references for the synergies development of the WH karst site integrity protection and agroforestry development in the buffer zone.
Distinct patterns of distribution, community assembly and cross-domain co-occurrence of planktonic archaea in four major estuaries of China
Authors: Hu Xuya , Huang Yujie , Gu Gaoke , Hu Hanjing , Yan Huizhen , Zhang Huajun , Zhang Rui , Zhang Demin , Wang Kai 
Published: October 2023

Abstract
Background
Archaea are key mediators of estuarine biogeochemical cycles, but comprehensive studies comparing archaeal communities among multiple estuaries with unified experimental protocols during the same sampling periods are scarce. Here, we investigated the distribution, community assembly, and cross-domain microbial co-occurrence of archaea in surface waters across four major estuaries (Yellow River, Yangtze River, Qiantang River, and Pearl River) of China cross climatic zones (~ 1,800 km) during the winter and summer cruises.

Results
The relative abundance of archaea in the prokaryotic community and archaeal community composition varied with estuaries, seasons, and stations (reflecting local environmental changes such as salinity). Archaeal communities in four estuaries were overall predominated by ammonia-oxidizing archaea (AOA) (aka. Marine Group (MG) I; primarily Nitrosopumilus), while the genus Poseidonia of Poseidoniales (aka. MGII) was occasionally predominant in Pearl River estuary. The cross-estuary dispersal of archaea was largely limited and the assembly mechanism of archaea varied with estuaries in the winter cruise, while selection governed archaeal assembly in all estuaries in the summer cruise. Although the majority of archaea taxa in microbial networks were peripherals and/or connectors, extensive and distinct cross-domain associations of archaea with bacteria were found across the estuaries, with AOA as the most crucial archaeal group. Furthermore, the expanded associations of MGII taxa with heterotrophic bacteria were observed, speculatively indicating the endogenous demand for co-processing high amount and diversity of organic matters in the estuarine ecosystem highly impacted by terrestrial/anthropogenic input, which is worthy of further study.

Conclusions
Our results highlight the lack of common patterns in the dynamics of estuarine archaeal communities along the geographic gradient, expanding the understanding of roles of archaea in microbial networks of this highly dynamic ecosystem.
Analysis of distribution, collection, and confirmation of capacity dependency of small extracellular vesicles toward a therapy for liver cirrhosis
Authors: Takeda Nobutaka , Tsuchiya Atsunori , Mito Masaki , Natsui Kazuki , Natusi Yui , Koseki Yohei , Tomiyoshi Kei , Yamazaki Fusako , Yoshida Yuki , Abe Hiroyuki , Sano Masayuki , Kido Taketomo , Yoshioka Yusuke , Kikuta Junichi , Itoh Tohru , Nishimura Ken , Ishii Masaru , Ochiya Takahiro , Miyajima Atsushi , Terai Shuji 
Published: October 2023

Abstract
Background
The progression of liver fibrosis leads to portal hypertension and liver dysfunction. However, no antifibrotic agents have been approved for cirrhosis to date, making them an unmet medical need. Small extracellular vesicles (sEVs) of mesenchymal stem cells (MSCs) are among these candidate agents. In this study, we investigated the effects of sEVs of MSCs, analyzed their distribution in the liver post-administration, whether their effect was dose-dependent, and whether it was possible to collect a large number of sEVs.

Methods
sEVs expressing tdTomato were generated, and their uptake into constituent liver cells was observed in vitro, as well as their sites of uptake and cells in the liver using a mouse model of liver cirrhosis. The efficiency of sEV collection using tangential flow filtration (TFF) and changes in the therapeutic effects of sEVs in a volume-dependent manner were examined.

Results
The sEVs of MSCs accumulated mostly in macrophages in damaged areas of the liver. In addition, the therapeutic effect of sEVs was not necessarily dose-dependent, and it reached a plateau when the dosage exceeded a certain level. Furthermore, although ultracentrifugation was commonly used to collect sEVs for research purposes, we verified that TFF could be used for efficient sEV collection and that their effectiveness is not reduced.

Conclusion
In this study, we identified some unknown aspects regarding the dynamics, collection, and capacity dependence of sEVs. Our results provide important fundamentals for the development of therapies using sEVs and hold potential implications for the therapeutic applications of sEV-based therapies for liver cirrhosis.
Research on anti-poverty efforts in China’s ethnic minority areas since the 1970s
Authors: Liu Shiyao 
Published: October 2023

AbstractPoverty remains a significant global challenge, despite ongoing efforts throughout history to address it. China’s fight against poverty, as part of global poverty governance efforts, has yielded valuable insights and examples for anti-poverty strategies, propelling changes within the global poverty governance system. This paper conducts a review of post-1978 anti-poverty research covering China’s ethnic minority regions, roughly dividing the research history into three distinct phases: 1978–2012, 2013–2020, and post-2020. The research findings at each stage are summarized in terms of research topics, perspectives, and paradigms. Based on these findings, the paper provides a forward-looking outlook on future research into anti-poverty efforts in China’s ethnic minority regions.
Sliding mode controller design via delay-dependent $$H_{\infty }$$ stabilization criterion for load frequency regulation
Authors: Pradhan Subrat Kumar , Das Dushmanta Kumar 
Published: October 2023

AbstractThis work presents a control approach based on sliding-mode-control (SMC) to design robust $$H_{\infty }$$

H
∞

state feedback controllers for load frequency regulation of delayed interconnected power system (IPS) with parametric uncertainties. Considering both state feedback control strategy and delayed feedback control strategy, two SMC laws are proposed. The proposed control laws are designed to improve the stability and disturbance rejection performance of delayed IPS, while stabilization criteria in the form of linear matrix inequality are derived by choosing a Lyapunov–Krasovskii functional. An artificial time-delay is incorporated in the control law design of the delayed feedback control structure to enhance the controller performance. A numerical example is considered to study the control performance of the proposed controllers and simulation results are provided to observe the dynamic response of the IPS.
Evicting and filling attack for linking multiple network addresses of Bitcoin nodes
Authors: Yang Huashuang , Shi Jinqiao , Gao Yue , Wang Xuebin , Sun Yanwei , Shi Ruisheng , Wang Dongbin 
Published: October 2023

AbstractBitcoin is a decentralized P2P cryptocurrency. It supports users to use pseudonyms instead of network addresses to send and receive transactions at the data layer, hiding users’ real network identities. Traditional transaction tracing attack cuts through the network layer to directly associate each transaction with the network address that issued it, thus revealing the sender’s network identity. But this attack can be mitigated by Bitcoin’s network layer privacy protections. Since Bitcoin protects the unlinkability of Bitcoin addresses and there may be a many-to-one relationship between addresses and nodes, transactions sent from the same node via different addresses are seen as coming from different nodes because attackers can only use addresses as node identifiers. In this paper, we proposed the evicting and filling attack to expose the correlations between addresses and cluster transactions sent from different addresses of the same node. The attack exploited the unisolation of Bitcoin’s incoming connection processing mechanism. In particular, an attacker can utilize the shared connection pool and deterministic connection eviction strategy to infer the correlation between incoming and evicting connections, as well as the correlation between releasing and filling connections. Based on inferred results, different addresses of the same node with these connections can be linked together, whether they are of the same or different network types. We designed a multi-step attack procedure, and set reasonable attack parameters through analyzing the factors that affect the attack efficiency and accuracy. We mounted this attack on both our self-run nodes and multi-address nodes in real Bitcoin network, achieving an average accuracy of 96.9% and 82%, respectively. Furthermore, we found that the attack is also applicable to Zcash, Litecoin, Dogecoin, Bitcoin Cash, and Dash. We analyzed the cost of network-wide attacks, the application scenario, and proposed countermeasures of this attack.
The Technique for Transorbital Ventricular Puncture: An Anatomic Approach
Authors: Soares Luís Gustavo Biondi , Holanda Vanessa Milanesi , Lages Gustavo Veloso , Soares Adria Gabrielle Biondi , Catarino Marcilio Monteiro , Ahumada-Vizcaíno Juan Carlos , Pereira Felipe Salvagni , Teixeira Maria Luiza Oliveira Lopes , Jiménez Luis Ángel Canache , Neto Mateus Reghin , Chaddad-Neto Feres 
Published: October 2023

BACKGROUND AND OBJECTIVES:
Transorbital ventricular puncture is a minimally invasive described procedure with poor landmarks and anatomic references. This approach can be easily performed to save patients with intracranial hypertension, especially when it is secondary to an acute decompensated hydrocephalus. This study aims to describe anatomic structures and landmarks to facilitate the execution of transorbital puncture in emergency cases.

METHODS:
We analyzed 120 head computed tomographies to show the best area to perform the procedure in the orbital roof. Two adult cadavers (4 sides) were punctured in the predetermined area. Angles, distances, landmarks, and anatomic structures were registered. This approach to the ventricular system may be performed at bedside to relieve intracranial hypertension only in specific cases.

RESULTS:
The perforation point is 2.5 cm (female) or 3.0 cm (male) lateral to the midline and immediately inferior to the superciliary arch. A vertical line, parallel to midline, was drawn on the outer edge of the patient's forehead, the needle was 45° inferiorly and 20° medially and then progressed 2.0 cm backwards to reach the bone perforation point. After that, it was advanced another 4.5cm approximately until it reached the anterior horn of the lateral ventricle.

CONCLUSION:
Based on statistical and experimental evidences, we were able to establish reliable anatomic reference points to access the anterior horn of the lateral ventricle through transorbital puncture.
Craniofacial developmental biology in the single-cell era
Authors: Tseng Kuo-Chang , Crump J. Gage 
Published: October 2023

ABSTRACT
The evolution of a unique craniofacial complex in vertebrates made possible new ways of breathing, eating, communicating and sensing the environment. The head and face develop through interactions of all three germ layers, the endoderm, ectoderm and mesoderm, as well as the so-called fourth germ layer, the cranial neural crest. Over a century of experimental embryology and genetics have revealed an incredible diversity of cell types derived from each germ layer, signaling pathways and genes that coordinate craniofacial development, and how changes to these underlie human disease and vertebrate evolution. Yet for many diseases and congenital anomalies, we have an incomplete picture of the causative genomic changes, in particular how alterations to the non-coding genome might affect craniofacial gene expression. Emerging genomics and single-cell technologies provide an opportunity to obtain a more holistic view of the genes and gene regulatory elements orchestrating craniofacial development across vertebrates. These single-cell studies generate novel hypotheses that can be experimentally validated in vivo. In this Review, we highlight recent advances in single-cell studies of diverse craniofacial structures, as well as potential pitfalls and the need for extensive in vivo validation. We discuss how these studies inform the developmental sources and regulation of head structures, bringing new insights into the etiology of structural birth anomalies that affect the vertebrate head.