bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic Field Emulations of Small Inhibitor RNA: Effects on Implanted GL261 Tumors in C57BL/6 Immune Competent Mice
Authors: Figueroa Xavier A. , Vogeli Gabriel , Butters Mike 
Published: December 2023

Abstract

Background: EMulate Therapeutics has developed a system for emulating the effects of small solvated molecules via their magnetic field recordings. Recordings of magnetic field emissions of select small inhibitor RNAs (siRNAs; murine targeting CTLA-4 and murine targeting PD-1) were tested on C57Bl/6 mice implanted subcutaneously with the GL261 murine tumor cell line.
Methods: A signal composed of the concatenated recordings of siRNA molecules targeting the murine CTLA-4 and PD-1 receptors (labeled A2) was used in immune competent C57Bl/6 mice. The mice were flank implanted with the murine glioblastoma cell line GL261. Mice were exposed to the signal continuously (24 hours a day) until tumor volumes reached the designated volume limit. Tumors were excised and analyzed via PAGE/Western blot for the expression of CTLA-4, PD-1, Ki67, Caspase 3, CD4 and CD8. Terminal blood draws were used for CBCs.
Results: We report the down regulation of the checkpoint inhibitors CTLA-4 in the exposed mice. Significant tumor volume reduction was observed in mice exposed to the siRNA signal compared to control mice; no adverse events were recorded. Cell blood counts (CBC) and protein expression patterns were observed to correlate with the expected function of protein expression inhibition of the targets.
Conclusions: The effect of the magnetic field emission targeting CTLA-4 and PD-1 demonstrates the ability to emulate the effects of siRNA and support previously published work using this technology.
The impact of moderate physical exercise, alone or combined with omega-3 supplementation, on apical periodontitis induced in rats.
Authors: Ribeiro Ana Paula Fernandes , Rodrigues Michely de Lima , Loureiro Caroline , Machado Nathalia Evelyn Silva , Silva Cristiane Cantiga , de Oliveira Pedro Henrique Chaves , Cintra Luciano Tavares Angelo , Jacinto Rogério Castilho 
Published: December 2023

Abstract
Objective: To investigate the impact of moderate-intensity physical exercise (PE), alone or in combination with omega-3 supplementation (O), on apical periodontitis (AP) in rats, by analyzing the inflammatory profile, and the presence and distribution of bacteria within the root canal and apical region.
Methods: Thirty Wistar rats divided into 3 groups: C; PE and PE+O. The exercise protocol occurred in two stages, acclimatization and swimming training. Omega-3 was administered via gavage during 60 days. AP was induced on the 30th day and the rats were euthanized on the 60th day. Maxillary molars were processed and stained with Hematoxylin-and-Eosin, immunohistochemistry for IL-17 and TNF-α and Brown-Brenn. The analyses were carried out by assigning scores submitted to Shapiro-Wilk, Kruskal-Wallis, and Tukey tests, with 5% significance.
Results: The intensity of the inflammatory infiltrate was higher in C group (p<0.05). PE alone reduced immunostaining for TNF-α and limited the spread of bacteria (p<0.05). Combined with omega-3 PE reduced immunostaining for IL-17.
Conclusion: PE sensitized the defense mechanisms, attenuating inflammation, through the modulation of TNF-α and contained bacterial contamination. PE+O significantly improved the modulating the inflammatory pathway by controlling IL-17.
Clinical Relevance: Recommending physical activity and the use of omega-3 to patients can result in more successful endodontic treatment.
Response of faba bean plants to drought stress and different plant density
Authors: Maaty Ahmed E. , Saad Salah M. , El-Hadary Abd Alla , Saad Ahmed M. 
Published: December 2023

Abstract
This study was carried out to examine and assess how the faba bean cultivar Marriott 2 (Vicia faba L.) responded to water stress and plant density.The effects of different faba bean planting densities per fed. (D1 = 20 kg, D2 = 30 kg,and D3 = 40 kg) and varied levels of drought stress (i.e. single irrigation,double irrigation,triple irrigation, and conventional irrigation) were studied.The obtained results illustrated that the total amount of chlorophyll might be badly impacted by a shortage of water. Additionally, a water deficit raised the contents of nitrogen, proteins, phosphorus, potassium, calcium, and proline while also, increasing the activity of the antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase. Furthermore,the outcomes demonstrated that it was significantly influenced by plant density,except calcium content and superoxide dismutase enzyme activity. At the level of interaction, all traits were significantly affected except the calcium content, throughout the winter growing season (2021–2022). Generally, the overall performance of the faba bean variety (cv. Marriott 2)was good and the results confirmed the existence of variations in the chemical composition with water regimes and various plant densities. Water deficit(three irrigation treatments) of water supplied up to75% of water holding capacity may slightly retard crop composition, this did not restrict the crop's ability to respond to their deviations in the chemical composition.Also, faba bean responded well at the optimum density 30kg/fed in all examined traits.
Language Maintenance and Attitudes among Members of the Garo Community in Bangladesh
Authors: Lecturer Jiniya Bhoraty , Mahbub Rahman M. 
Published: December 2023

Abstract
Bangladesh is a culturally diverse nation, with a visible presence of numerous communities. The Garo community is one of them. The Garo people possess a culturally and linguistically diverse heritage. Despite facing significant pressure from national and global influences on language and culture, they persist in preserving and practising their own language and culture. The Garo language is primarily spoken and lacks a written form. Since its inception, they have been practising orally and there have been no language preservation issues. Following independence, the Bengali language was designated as the national language, obligating all individuals in Bangladesh, regardless of their linguistic heritage, including the Garo population, to communicate in the national language. Consequently, all ethnic languages, including Garo, are currently in danger. While a few researchers have begun to work on the Garo language, no studies have focused on the ways in which the Garo people themselves are maintaining their native language. Maintaining different languages and cultures in a monolingual society is a challenging endeavour. This study demonstrates the present state of the Garo language in Bangladesh, as well as the attitudes towards language preservation among its speakers. It is a comprehensive analysis. Collected data has been obtained using semi-structured interviews. In order to demonstrate language preservation, many domains such as family, friends, literacy, and religion have been chosen. Additionally, participants were asked additional questions regarding their attitudes to ascertain their levels of support or opposition towards the usage and preservation of the Garo language. It is found that the domain of relatives and family members plays a vital role in the preservation of the Garo language, whereas the influence of other domains is relatively less significant.
Development of TiN-Based Suspended Meander Kinetic Inductance Detectors (KIDs) for Visible and Near-Infrared Astronomy Applications
Authors: Appavou Maria , Ribeiro Lucas , Nicaise Paul , Hu Jie , Martin Jean-Marc , Firminy Josiane , Chaumont Christine , Bonifacio Piercarlo , Boussaha Faouzi 
Published: December 2023

Abstract
We propose high-Q factors sub-stoichiometric TiN-based Lumped Element MKIDs on sapphire with vacuum gap suspended meander. The aim is to improve detector response by trapping phonons within the meander to increase the number of Cooper pairs breaking into quasiparticles. Furthermore, either Nb/Au or Nb/Al reflective thin bilayer can be placed beneath \textcolor{black}{the meander} to respectively improve the photon peak absorption at the near-infrared and visible wavelengths.
Prevalence of and factors associated with anxiety, depression and post-traumatic stress disorder among Sudan ebolavirus disease survivors and family members, Uganda, January 2023: a cross-sectional study
Authors: Agaba Brian , Nelly Hellen Naiga , Wanyana Mercy Wendy , Zalwango Jane Frances , Simbwa Brenda , Akunzirwe Rebecca , Zavuga Robert , Kawungezi Peter Chris , Zalwango Marie Gorreti , Migamba Stella Martha , Kadobera Daniel , Bulage Lilian , Migisha Richard , Ario Alex Riolexus , Harris Julie R. 
Published: December 2023

Abstract

Background
Communities affected by Ebola disease (EBOD) may face resulting increases in mental health disorders. We evaluated the prevalence of and factors associated with mental health disorders among persons affected by the 2022 Sudan virus disease (SVD) outbreak in Uganda.
Methods
We conducted a cross-sectional study among SVD survivors and family members of survivors and fatal cases from 15–31 January 2023 (six weeks after the last case was discharged). We included only laboratory-confirmed, consenting SVD survivors and family members who lived with or cared for confirmed SVD patients during their illness and who were home at the time of our visits. The Hospital Anxiety and Depression Scale was used to evaluate anxiety and depression. The post-traumatic stress disorder (PTSD) checklist for the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition was used to evaluate PTSD. We used modified Poisson regression (adjusted for clustering by household) to determine factors associated with each mental health disorder.
Results
We enrolled 54 survivors and 82 family members from 96 households; median participant age was 30 years (range, 15–73), and 54% were female. The prevalence of anxiety (55%) and depression (50%) was higher than PTSD (17%); 64% had ≥ 1 mental health disorder. The prevalence of anxiety among persons from households with 2–4 members (aPR = 0.54, 95%CI:0.31–0.92) was lower than among persons who lived alone. The prevalence of PTSD was lower in persons from households with 2–4 members (aPR = 0.24, 95%CI:0.08–0.66) and > 4 members (aPR = 0.32, 95%CI:0.13–0.78) compared to persons who lived alone. Persons from households with ≥ 1 SVD death had a higher prevalence of depression (aPR = 1.8, 95%CI:1.1–3.3) and anxiety (aPR = 1.9, 95%CI:1.1–3.5) compared to households with no EBOD deaths. The prevalence of all mental health disorders was similar between survivors and family members.
Conclusion
Approximately two-thirds of SVD survivors and family members of patients in the 2022 outbreak in Uganda had ≥ 1 mental health disorder shortly after the outbreak ended. Strengthening mental health services during and after Ebola virus outbreaks for survivors and family members of patients may enhance the quality of outbreak response.
Deep learning-based computed tomography assessment for lung function prediction in chronic obstructive pulmonary disease
Authors: Shimizu Kaoruko , Sugimori Hiroyuki , Tanabe Naoya , Wakazono Nobuyasu , Ito Yoichi , Takahashi Keita , Makita Hironi , Sato Susumu , Suzuki Masaru , Nishimura Masaharu , Hirai Toyohiro , Konno Satoshi 
Published: December 2023

Abstract
Deep learning models based on medical imaging enable numerical functional predictions in combination with regression methods. In this study, we evaluate the prediction performance of a deep learning-based model for the raw value and percent predicted forced expiratory volume in one second (FEV1) in patients with chronic obstructive pulmonary disease (COPD). To this end, ResNet50-based regression prediction models were constructed for FEV1 and %FEV1 based on 200 CT scans. 10-fold cross-validation was performed to yield ten models in aggregate. The prediction model for %FEV1 was externally validated using 20 data points. Two hundred internal CT datasets were assessed using commercial software, producing a regression model predicting airway [%WA] and parenchymal indices [%LAV]. The average Root Mean Squared Error(RMSE) value of the 10 predictive models was 627.65 for FEV1 as per internal validation and 15.34 for %FEV1. The externally validated RMSE for %FEV1 was 11.52, whereas that for %FEV1 was 23.18. The predictive model for %FEV1 yielded significant positive correlations corresponding to both internal and external validation. The proposed models exhibited better prediction accuracy for %FEV1 than for FEV1. Further studies are required to improve the accuracy further and determine the validity of longitudinal applications.
Study of the Side effects resulting from inhalation sprays among patients in Damascus Hospital
Authors: Kasas Kholud , Al-Gusain Rama , Mardini Heba 
Published: December 2023

Abstract
Introduction:
The side effects of inhalation sprays are minimal or non-existent if they are used correctly and in low doses, but if they are used randomly, many side effects occur, including inflammation of the mouth or throat, a hoarse or croaking voice, coughing, and oral thrush.
Materials and methods:
Observational study with the type of cross-sectional study. This study targeted patients attending Damascus Hospital. The sample was selected randomly, and about (113) patients were elected who met specific acceptance criteria, the most important of which was the presence of sufficient information in the file, while all patients were excluded according to specific exclusion criteria. Data related to this study were collected using paper Questionnaire forms. These forms were designed by the researcher responsible for the research under the supervision of the professor responsible for the thesis. The information necessary to fill out these forms was collected by returning to the files of the Thoracic Department in the archives of Damascus Hospital. This data from paper questionnaires was entered into a SPSS file, version 25, to be analyzed and results for this study were generated.
Results:
What we found in this study is that the appearance of side effects from inhalation sprays is not correlated to the gender of the patient. There is an important correlation between the age of the patient and the appearance of side effects from inhalation sprays. There is an important correlation between the patient’s profession and the appearance of side effects from inhalation sprays. The appearance of side effects from inhalation sprays is not related to the environment where the patient lives, and there is also an important correlation between smoking and the appearance of side effects from inhalation sprays, and there is an important correlation between the patient’s suffering from a chronic disease and the appearance of side effects from inhalation sprays, and the appearance of side effects is not related to the type of inhaler, and there is an important correlation between the number of times the inhaler is used ( daily dose) and the appearance of side effects from inhaler sprays, and the appearance of side effects is not related to following preventive behaviors.
Recommendations:
We recommend quitting smoking and staying away from it, adopting protective behavior, being away from allergens and dust in the workplace, and reviewing the doctor periodically and adjusting the appropriate dose of the inhaler.
Effects of Nanographene oxide on adipose-derived stem cell cryopreservation
Authors: Li Zifei , Qi Jun , Fu Su , Luan Jie , Wang Qian 
Published: December 2023

Abstract

Cryoinjury mitigation is key in cell cryopreservation. Here, we aimed to assess the effectiveness of nanographene oxide (nano-GO) for improving cryoprotectant agents (CPAs) in human adipose stem cell (hADSC) cryopreservation. For in vitro experiments, nano-GO (5 µg/mL) was added to the CPAs in the control, and passage (P) 2 hADSCs were collected and cryopreserved for around two weeks. We compared cytotoxicity, cell viability, immunophenotypes, proliferation, cell apoptosis, and tri-lineage differentiation. In vivo studies used lipoaspirate to create non-enriched or hADSC-enriched fat tissues by combining it with PBS or hADSCs cryopreserved with the aforementioned CPAs. Each nude mouse received a 0.3 mL subcutaneous injection of the graft. At 12 weeks, the grafts were harvested. Histology, adipocyte-associated genes and protein, vascular density and angiogenic cytokines, macrophage infiltration, and inflammatory cytokines were analyzed. Nano-GO CPA contributed to increased cell viability, improved cell recovery, and lowered levels of early apoptosis. Nano GO at concentrations of 0.01 to 100 µg/mL caused no cytotoxicity to hADSCs. The absence of nano GOs in the intracellular compartments of the cells was confirmed by transmission electron microscopy. The fat grafts from the CPA-GO group showed more viable adipocytes and significantly increased angiogenesis compared to the PBS and CPA-C groups. Adding hADSCs from the CPA-GO group to the graft reduced macrophage infiltration and MCP-1 expression. Nano-GO plays an anti-apoptotic role in the cryopreservation of hADSCs, which could improve the survival of transplanted fat tissues, possibly via improved angiogenesis and lower inflammatory response in the transplanted adipose tissue.
Black TiO 2 -based nanoparticles as Toll-like receptor stimulators delivery system for enhanced photothermal-immunotherapy of pancreatic cancer
Authors: Xu Liu , Wu Ruoyu , Ni Jiajing , Jin Lufei , Xu kaiwei , Zhu Yuchao , Hong Lu , Chen Chunqu , Wang Linwei , Zhu Lubing , Zhou Weijian , Shen Wenqi , Wang Jianhua 
Published: December 2023

Abstract
Background
The tumor-specific immune responses, essential for removing residual lesions and preventing tumor metastases, can be stimulated by tumor-associated antigens (TAAs) released following photothermal therapy (PTT). However, due to the immunosuppressed microenvironment of pancreatic ductal adenocarcinoma (PDAC), the TAAs released by PTT are difficult to induce an effective immune response. In this work, we prepared the mesoporous silica (mSiO2) coated black titanium dioxide (bTiO2) photothermal nanoparticles (NPs) for enhanced photothermal-immunotherapy toward PDAC, in which resiquimod (R848) was loaded and DOTA-Gd was conjugated. The NPs are specified as bTiO2@mSiO2@Gd/R848 and abbreviated to NPs/R848. R848 as a kind of Toll-like receptor 7/8 agonist can induce a strong immune response against tumors. Furthermore, DOTA-Gd serves as a magnetic resonance imaging (MRI) contrast agent to improve the T1-weighted MRI performance of the NPs.
Results
In vitro results of this study show that NPs/R848 could thermally ablate tumor cells and efficiently trigger dendritic cells (DCs) maturation. The results of in vivo investigations demonstrate that the combined use of photothermal-immunotherapy exhibits a significant inhibitory effect on tumor growth. Besides, it promoted maturation of DCs and enhanced infiltration of CD8+, CD4 + T cells to improve the tumor microenvironment.
Conclusions
Our study anticipate that by encouraging the maturation of DCs, this strategy will enhance the tumor microenvironment and enable the successful photothermal-immunotherapy of PDAC.
The relationship between inflammatory markers from blood routine test and colorectal polyps
Authors: Yin Wen , Huang Yaochan , Gao Feng , Ye Xiaoyan , Zhang Yuqi , Wu Congye , Yuan Jie 
Published: December 2023

Abstract
Objective: The aim of this study was to investigate the relationship betweeninflammatory markers and colorectal polyps.
Methods:The inflammatory markers included neutrophils to lymphocytes ratio (NLR), platelets to lymphocytes ratio (PLR), lymphocytes to monocytes ratio (LMR), and red blood cell distribution width (RDW). A total of 4963 patientsunderwent gastrointestinal endoscopy were retrospectively analyzed. The inflammation markers were compared between the polyp group and the normal group. The pathological types, numbers, and diameter of polyps were analyzed between high and low inflammatory marker groups.
Results: The NLR, PLR, and RDW values in the polyp group were higher when compared to those in the normal group, while the LMR value was lower (P <0.05). The incidence rates of adenomas were higher in the high NLR and high RDW groups when compared to the low NLR and low RDW groups. Similarly, the incidence rates of adenomas were higher in the low LMR group compared to the high LMR group (P <0.05). However, there was no significant correlationbetween the level of inflammatory markers and the number or diameter of polyps (P >0.05).
Conclusion: There is a correlation between inflammatory markers and colorectal polyps, particularly in patients with adenoma as opposed to non-adenoma patients. However, there is no clear correlation between the diameter and number of polyps and these inflammatory markers.
Dynamics and Analysis of Dengue Epidemics: A Fractional Order SIR Model with Next-Generation Matrix Methodology
Authors: Alfalqi Suleman , Niazi Azmat Ullah Khan , Ramay Easha , Ahmed Bilal , Alhagyan Mohammed 
Published: December 2023

Abstract
One of the major infectious diseases in the world is dengue. Dengue fever, a mosquito-borne viral infection, continues to pose a significant public health threat in tropical and subtropical countries. In this article, we analyze the susceptible-infected-recuperated (SIR) model-based fractional order differential equation of the dengue epidemic system and we analyze of fractional model for the dengue transmission utilizing the new term Human hospitalized. The next-generation matrix methodology is used to obtain the threshold quantity value $R_{0}$, which is comparable to the essential reproduction value. In the case of constant controls, we evaluate the presence and consistency of the disease-free and infectious equilibrium simulation alternatives. The disease-free equilibrium (DFE) point and the endemic equilibrium point's local stability are discussed. We were able to determine that DFE is locally asymptotically stable when $R_{0}<1$ and unstable when $R_{0}>1$ by applying the linearization theorem. Numerical simulations are conducted to validate the proposed model against real-world dengue data. For various parameter values of the derivative $\alpha$ order, numerical simulations are provided. The proposed model is validated using published every year (390 millions) dengue cases are reported in world wide. The presented model, it is noted, offers a more accurate means of analyzing the dynamics of the disease dengue.
2010 MSC: 26A33, 34K37.