bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glc1.8 fromMicroplitis demolitorBracovirus Induces a Loss of Adhesion and Phagocytosis in Insect High Five and S2 Cells
Authors: Beck Markus , Strand Michael R. 
Published: January 2005

ABSTRACTPolydnaviridaeis a unique family of DNA viruses that are symbiotically associated with parasitoid wasps. Upon oviposition, wasps inject these viruses into their hosts, where they cause several physiological alterations, including suppression of the cellular immune response. Here we report that expression of theglc1.8gene fromMicroplitis demolitorbracovirus (MdBV) causes a loss of adhesion by two hemocyte-like cell lines, namely, High Five cells from the lepidopteranTrichoplusia niand S2 cells from the dipteranDrosophila melanogaster. The expression of recombinant Glc1.8 also greatly reduced the ability of these cells to phagocytize foreign targets. Glc1.8 is characterized by a signal peptide at its N terminus, an extracellular domain comprised of five nearly perfect tandem repeats of 78 amino acids, and a C-terminal hydrophobic domain that encodes a putative membrane anchor sequence. The expression of a Glc1.8 mutant lacking the anchor sequence resulted in a secreted protein that had no effect on adhesion or phagocytosis. In contrast, sequential deletion of the repeats in the extracellular domain resulted in a progressive reduction in immunosuppressive activity. Since each repeat and its associated glycosylation sites are nearly identical, these results suggested that adhesion-blocking activity depends more on the overall number of repeats in the extracellular domain than on the specific determinants within each repeat. While it severely compromised adhesion and phagocytic functions, Glc1.8 did not cause cell death. Collectively, these results indicate that Glc1.8 is a major pathogenic determinant of MdBV that is involved in suppression of the insect cellular immune response.
Exposure of RNA Templates and Encapsidation of Spliced Viral RNA Are Influenced by the Arginine-Rich Domain of Human Hepatitis B Virus Core Antigen (HBcAg 165-173)
Authors: Le Pogam Sophie , Chua Pong Kian , Newman Margaret , Shih Chiaho 
Published: January 2005

ABSTRACT

Previously, human hepatitis B virus
(
HBV) mutant 164, which has a truncation at the C terminus of the HBV core antigen (HBcAg), was speculated to secrete immature genomes. For this study, we further characterized mutant 164 by different approaches. In addition to the 3.5-kb pregenomic RNA (pgRNA), the mutant preferentially encapsidated the 2.2-kb or shorter species of spliced RNA, which can be reverse transcribed into double-stranded DNA before virion secretion. We observed that mutant 164 produced less 2.2-kb spliced RNA than the wild type. Furthermore, it appeared to produce at least two different populations of capsids: one encapsidated a nuclease-sensitive 3.5-kb pgRNA while the other encapsidated a nuclease-resistant 2.2-kb spliced RNA. In contrast, the wild-type core-associated RNA appeared to be resistant to nuclease. When arginines and serines were systematically restored at the truncated C terminus, the core-associated DNA and nuclease-resistant RNA gradually increased in both size and signal intensity. Full protection of encapsidated pgRNA from nuclease was observed for HBcAg 1-171. A full-length positive-strand DNA phenotype requires positive charges at amino acids 172 and 173. Phosphorylation at serine 170 is required for optimal RNA encapsidation and a full-length positive-strand DNA phenotype. RNAs encapsidated in
Escherichia coli
by capsids of HBcAg 154, 164, and 167, but not HBcAg 183, exhibited nuclease sensitivity; however, capsid instability after nuclease treatment was observed only for HBcAg 164 and 167. A new hypothesis is proposed here to highlight the importance of a balanced charge density for capsid stability and intracapsid anchoring of RNA templates.
Neuroinvasion by Scrapie following Inoculation via the Skin Is Independent of Migratory Langerhans Cells
Authors: Mohan Joanne , Bruce Moira E. , Mabbott Neil A. 
Published: January 2005

ABSTRACTMany natural transmissible spongiform encephalopathy (TSE) infections are likely to be acquired peripherally, and studies in mice show that skin scarification is an effective means of scrapie transmission. After peripheral exposure, TSE agents usually accumulate in lymphoid tissues before spreading to the brain. The mechanisms of TSE transport to lymphoid tissues are not known. Langerhans cells (LCs) reside in the epidermis and migrate to the draining lymph node after encountering antigen. To investigate the potential role of LCs in scrapie transportation from the skin, we utilized mouse models in which their migration was blocked either due to CD40 ligand deficiency (CD40L−/−mice) or after caspase-1 inhibition. We show that the early accumulation of scrapie infectivity in the draining lymph node and subsequent neuroinvasion was not impaired in mice with blocked LC migration. Thus, LCs are not involved in TSE transport from the skin. After intracerebral inoculation with scrapie, wild-type mice and CD40L−/−mice develop clinical disease with similar incubation periods. However, after inoculation via skin scarification CD40L−/−mice develop disease significantly earlier than do wild-type mice. The shorter incubation period in CD40L−/−mice is unexpected and suggests that a CD40L-dependent mechanism is involved in impeding scrapie pathogenesis. In vitro studies demonstrated that LCs have the potential to acquire and degrade protease-resistant prion protein, which is thought to be a component of the infectious agent. Taken together, these data suggest that LCs are not involved in scrapie transport to draining lymphoid tissues but might have the potential to degrade scrapie in the skin.
Attenuating Mutations of the Matrix Gene of Influenza A/WSN/33 Virus
Authors: Liu Teresa , Ye Zhiping 
Published: January 2005

ABSTRACT
The matrix protein (M1) of influenza virus plays an essential role in viral replication. Our previous studies have shown that basic amino acids 101RKLKR105 of M1 are involved in RNP binding and nuclear localization. For the present work, the functions of 101RKLKR105 were studied by introducing mutations into the M gene of influenza virus A/WSN/33 by reverse genetic methods. Individual substitution, R101S or R105S, had a minimal effect on viral replication. In contrast, the double mutation R101S-R105S was synergistic and resulted in temperature sensitivity reflected by reduced viral replication at a restrictive temperature. To investigate the in vivo effect on infection, BALB/c mice were infected with either A/WSN/33 wild-type (Wt) or mutant viruses and assessed for signs of illness, viral replication in the lungs, and survival rates. The results from mouse studies indicated that the R101S-R105S double mutant virus was strongly attenuated, while single mutant viruses R101S and R105S were minimally attenuated compared to A/WSN33 Wt under the same conditions. In challenge studies, mice immunized by infection with R101S-R105S were fully protected from lethal challenge with A/WSN/33. The replication and attenuating properties of R101S-R105S suggest its potential in development of live influenza virus vaccines.
Inhibition of Interferon Signaling by the New York 99 Strain and Kunjin Subtype of West Nile Virus Involves Blockage of STAT1 and STAT2 Activation by Nonstructural Proteins
Authors: Liu Wen Jun , Wang Xiang Ju , Mokhonov Vladislav V. , Shi Pei-Yong , Randall Richard , Khromykh Alexander A. 
Published: January 2005

ABSTRACTThe interferon (IFN) response is the first line of defense against viral infections, and the majority of viruses have developed different strategies to counteract IFN responses in order to ensure their survival in an infected host. In this study, the abilities to inhibit IFN signaling of two closely related West Nile viruses, the New York 99 strain (NY99) and Kunjin virus (KUN), strain MRM61C, were analyzed using reporter plasmid assays, as well as immunofluorescence and Western blot analyses. We have demonstrated that infections with both NY99 and KUN, as well as transient or stable transfections with their replicon RNAs, inhibited the signaling of both alpha/beta IFN (IFN-α/β) and gamma IFN (IFN-γ) by blocking the phosphorylation of STAT1 and its translocation to the nucleus. In addition, the phosphorylation of STAT2 and its translocation to the nucleus were also blocked by KUN, NY99, and their replicons in response to treatment with IFN-α. IFN-α signaling and STAT2 translocation to the nucleus was inhibited when the KUN nonstructural proteins NS2A, NS2B, NS3, NS4A, and NS4B, but not NS1 and NS5, were expressed individually from the pcDNA3 vector. The results clearly demonstrate that both NY99 and KUN inhibit IFN signaling by preventing STAT1 and STAT2 phosphorylation and identify nonstructural proteins responsible for this inhibition.
Transcriptional Activity of Baculovirus Very Late Factor 1
Authors: Mistretta Toni-Ann , Guarino Linda A. 
Published: January 2005

ABSTRACT

The product of the
vlf-1
(very late factor 1) gene is required for expression of very late genes during the final phase of infection. To determine whether VLF-1 functions as a transcriptional activator, VLF-1 was overexpressed and purified by affinity and cation exchange chromatography. The addition of purified protein to transcription assays containing baculovirus RNA polymerase stimulated transcription of the very late polyhedrin promoter but not the late
39k
promoter. Furthermore, construction and analysis of chimeric templates identified sequences within the polyhedrin promoter that were necessary for enhancement.
Human Herpesvirus 6B Induces Cell Cycle Arrest Concomitant with p53 Phosphorylation and Accumulation in T Cells
Authors: Øster Bodil , Bundgaard Bettina , Höllsberg Per 
Published: January 2005

ABSTRACT

We studied the interactions between human herpesvirus 6B (HHV-6B) and its host cell. Productive infections of T-cell lines led to G
1
/S- and G
2
/M-phase arrest in the cell cycle concomitant with an increased level and enhanced DNA-binding activity of p53. More than 70% of HHV-6B-infected cells did not bind annexin V, indicating that the majority of cells were not undergoing apoptosis. HHV-6B infection induced Ser20 and Ser15 phosphorylation on p53, and the latter was inhibited by caffeine, an ataxia telangiectasia mutated kinase inhibitor. Thus, a productive HHV-6B infection suppresses T-cell proliferation concomitant with the phosphorylation and accumulation of p53.
Atomic Force Microscopy Investigation of Isolated Virions of Murine Leukemia Virus
Authors: Kuznetsov Y. G. , Low A. , Fan H. , McPherson A. 
Published: January 2005

ABSTRACT
Virions of mouse leukemia virus spread on glass substrates were visualized by atomic force microscopy. The size distribution mode was 145 nm, significantly larger than that for human immunodeficiency virus particles. The distribution of particle sizes is broad, indicating that no two particles are likely identical in content or surface features. Virions possess knoblike protrusions, which may represent vestiges of budding from cell membranes. Particles which split open allowed imaging of intact cores with diameters of 65 nm. They also permitted estimation of viral shell thickness (35 to 40 nm) and showed the presence of a distinct trough between the shell and the core surface.
Is the rate of biological aging, as measured by age at diagnosis of cancer, socioeconomically patterned?
Authors: Adams Jean , White Martin , Forman David 
Published: January 2005

Study objective:To investigate the hypothesis that biological aging, as measured by age at diagnosis of some common cancers, is socioeconomically patterned.Design:A cross sectional analysis of the association between an area based measure of material deprivation and age at diagnosis of four common cancers (breast, prostate, colorectal, and lung cancers). A further analysis, restricted to breast and colorectal cancer, adjusted for stage and grade of cancer at diagnosis.Setting:The Northern and Yorkshire cancer registry and information service, Northern and Yorkshire region, UK.Participants:All people living in the Northern and Yorkshire region diagnosed with breast, prostate, colorectal, or lung cancer in 1986–1995. All people living in the Northern and Yorkshire region diagnosed with breast or colorectal cancer in 1998–2000 with data on stage and grade of cancer at diagnosis.Main results:There was evidence that greater material deprivation was associated with younger age at diagnosis of cancer in prostate (β coefficient −0.073), colorectal (women: −0.042; men: −0.063), and lung cancer (women: −0.214; men: −0.161). The opposite association was found in women with breast cancer (0.149). Adjusting for stage and grade at incidence, where possible, had little effect on the magnitude of the β coefficients.Conclusions:Age at diagnosis of some common cancers seems to be socioeconomically patterned with people from more deprived areas being diagnosed with prostate, colorectal, and lung cancers earlier in life. The opposite was seen in women with breast cancer. Further work is required to investigate the socioeconomic distribution of more accurate measures of biological aging.
Childhood cancers and atmospheric carcinogens
Authors: Knox E G 
Published: January 2005

Study objectives:To retest previous findings that childhood cancers are probably initiated by prenatal exposures to combustion process gases and to volatile organic compounds (VOCs); and to identify specific chemical hazards.Design:Birth and death addresses of fatal child cancers in Great Britain between 1966 and 1980, were linked with high local atmospheric emissions of different chemical species. Among migrant children, distances from each address to the nearest emissions “hotspot” were compared. Excesses of outward over inward migrations show an increased prenatal or early infancy risk.Setting and subjects:Maps of emissions of many different substances were published on the internet by the National Atmospheric Emissions Inventory and “hotspots” for 2001 were translated to map coordinates. Child cancer addresses were extracted from an earlier inquiry into the carcinogenic effects of obstetric radiographs; and their postcodes translated to map references.Main results:Significant birth proximity relative risks were found within 1.0 km of hotspots for carbon monoxide, PM10 particles, VOCs, nitrogen oxides, benzene, dioxins, 1,3-butadiene, and benz(a)pyrene. Calculated attributable risks showed that most child cancers and leukaemias are probably initiated by such exposures.Conclusions:Reported associations of cancer birth places with sites of industrial combustion, VOCs uses, and associated engine exhausts, are confirmed. Newly identified specific hazards include the known carcinogens 1,3-butadiene, dioxins, and benz(a)pyrene. The mother probably inhales these or related materials and passes them to the fetus across the placenta.
Aerosol Characterization and Direct Radiative Forcing Assessment over the Ocean. Part I: Methodology and Sensitivity Analysis
Authors: Costa Maria João , Silva Ana Maria , Levizzani Vincenzo 
Published: January 2005

Abstract
A method based on the synergistic use of low earth orbit (LEO) and geostationary earth orbit (GEO) satellite data for aerosol-type characterization, as well as aerosol optical thickness (AOT) retrieval and monitoring over the ocean, is presented. These properties are used for the estimation of the direct shortwave aerosol radiative forcing at the top of the atmosphere. The synergy serves the purpose of monitoring aerosol events at the GEO time and space scales while maintaining the accuracy level achieved with LEO instruments. Aerosol optical properties representative of the atmospheric conditions are obtained from the inversion of high-spectral-resolution measurements from the Global Ozone Monitoring Experiment (GOME). The aerosol optical properties are input for radiative transfer calculations for the retrieval of the AOT from GEO visible broadband measurements, avoiding the use of fixed aerosol models available in the literature. The retrieved effective aerosol optical properties represent an essential component for the aerosol radiative forcing assessment. A sensitivity analysis is also presented to quantify the effects that changes on the aerosol model may have on modeled results of spectral reflectance, AOT, and direct shortwave aerosol radiative forcing at the top of the atmosphere. The impact on modeled values of the physical assumptions on surface reflectance and vertical profiles of ozone and water vapor are analyzed. Results show that the aerosol model is the main factor influencing the investigated radiative variables. Results of the application of the method to several significant aerosol events, as well as their validation, are presented in a companion paper.
The Role of Airmass Types and Surface Energy Fluxes in Snow Cover Ablation in the Central Appalachians
Authors: Leathers Daniel J. , Graybeal Daniel , Mote Thomas , Grundstein Andrew , Robinson David 
Published: January 2005

Abstract
A one-dimensional snowpack model, a unique airmass identification scheme, and surface weather observations are used to investigate large ablation events in the central Appalachian Mountains of North America. Data from cooperative observing stations are used to identify large ablation events within a 1° latitude × 1° longitude grid box that covers the majority of the Lycoming Creek basin in northern Pennsylvania. All 1-day ablation events greater than or equal to 7.6 cm (3 in.) are identified for the period of 1950 through 2001. Seventy-one events are identified, and these days are matched with a daily airmass type derived using the Spatial Synoptic Classification technique. Average meteorological characteristics on ablation days of each airmass type are calculated in an effort to understand the diverse meteorological influences that led to the large ablation events. A one-dimensional mass and energy balance snowpack model (“SNTHERM”) is used to calculate surface/atmosphere energy fluxes responsible for ablation under each airmass type. Results indicate that large ablation events take place under diverse airmass/synoptic conditions in the central Appalachians. Five airmass types account for the 71 large ablation events over the 52-yr period. Forty-three of the events occurred under “moist” airmass types and 28 under “dry” airmass conditions. Large ablation events under dry airmass types are driven primarily by daytime net radiation receipt, especially net solar radiation. These events tend to occur early and late in the snow cover season when solar radiation receipt is highest and are characterized by relatively clear skies, warm daytime temperatures, and low dewpoint temperatures. Moist airmass types are characterized by cloudy, windy conditions with higher dewpoint temperatures and often with liquid precipitation. During these events sensible heat flux is most often the dominant energy flux to the snowpack during ablation episodes. However, in many cases there is also a significant input of energy to the snowpack associated with condensation. Combinations of high sensible and latent heat fluxes often result in extreme ablation episodes, similar to those witnessed in this area in January 1996.
Estimating Overwater Turbulence Intensity from Routine Gust-Factor Measurements
Authors: Hsu S. A. , Blanchard Brian W. 
Published: January 2005

AbstractFor overwater diffusion estimates the Offshore and Coastal Dispersion (OCD) model is preferred by the U.S. Environmental Protection Agency. The U.S. Minerals Management Service has recommended that the OCD model be used for emissions located on the outer continental shelf. During southerly winds over the Gulf of Mexico, for example, the pollutants from hundreds of offshore platforms may affect the gulf coasts. In the OCD model, the overwater plume is described by the Gaussian equation, which requires the computation of σy and σz, which are, in turn, related to the turbulence intensity, overwater trajectory, and atmospheric stability. On the basis of several air–sea interaction experiments [the Barbados Oceanographic and Meteorological Experiment (BOMEX), the Air-Mass Transformation Experiment (AMTEX), and, most recently, the Tropical Ocean and Global Atmosphere Coupled Ocean–Atmosphere Response Experiment (TOGA COARE)] and the extensive datasets from the National Data Buoy Center (NDBC), it is shown that under neutral and stable conditions the overwater turbulence intensities are linearly proportional to the gust factor (G), which is the ratio of the wind gust and mean wind speed at height z (Uz) as reported hourly by the NDBC buoys. Under unstable conditions, it is first shown that the popular formula relating the horizontal turbulence intensity (σu,υ/u∗, where u∗ is the friction velocity) to the ratio of the mixing height (h) and the buoyancy length (L) (i.e., h/L) suffers from a self-correlation problem and cannot be used in the marine environment. Then, alternative formulas to estimate the horizontal turbulence intensities (σu,υ/Uz) using G are proposed for practical applications. Furthermore, formulas to estimate u∗ and z/L are fundamentally needed in air–sea interaction studies, in addition to dispersion meteorology.
An Idealized Study of the Structure of Long, Partially Mixed Estuaries*
Authors: Hetland Robert D. , Geyer W. Rockwell 
Published: January 2005

Abstract
Classic models of estuarine circulation are reexamined using a three-dimensional, primitive equation numerical ocean model. The model is configured using an idealized estuary/shelf domain with rectangular cross section, constant vertical mixing, and steady riverine discharge. Tidal dispersion is neglected, so the analysis does apply to well-mixed estuaries and lagoons. Estuarine scales for the length of steady-state salt intrusion, vertical stratification, and estuarine exchange flow estimated from steady-state model results are found to have the same functional relationships to vertical mixing and riverine discharge as the classic analytic solutions. For example, for steady-state conditions, the stratification is found to be virtually independent of the strength of vertical mixing. The estuarine structure was controlled by the interior estuarine circulation, and not by limited exchange at the mouth. Thus, the numerical solutions were not “overmixed,” although the solutions showed a dependence on freshwater flux functionally similar to the overmixed solution. Estuarine adjustment time scales are also estimated from the simulations, and they are related to the steady-state estuarine scales. Two classes of nonsteady solutions are examined: the response to a step change in riverine discharge and estuarine response to changes in vertical mixing. Spring/neap tidal variations are examined by modulating the (spatially constant) vertical mixing with a fortnightly period. Unlike the steady solutions, there is a clear dependence of stratification on mixing rate in the time-dependent solutions. The simulations involving changes in riverine discharge show asymmetries between response to increasing and decreasing river flow that are attributed to quadratic bottom drag.