bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical analysis of effects of industrial processing steps on functional properties of pasteurised liquid egg white
Authors: Lechevalier Valérie , Périnel Emmanuel , Jeantet Romain , Lesaffre Capucine , Croguennec Thomas , Guérin‐Dubiard Catherine , Nau Françoise 
Published: December 2004

AbstractEgg white is widely used as an ingredient in the food industry owing to its excellent functional properties. The transformation of shell eggs into safe liquid, frozen, or spray‐dried egg white with extended shelf life requires many technological operations that result in modifications to the egg white's functional properties. The present study highlights the critical steps affecting foaming and gelling properties during a classical pasteurised liquid egg white process. The main source of variation in functional properties was raw material quality, accounting for 70% of the variability. Part of the remaining 30% was explained by mechanical egg white–yolk separation, tank storage, pasteurisation and homogenisation that resulted in damage to the functional properties, whereas initial flow through pipes and pumping resulted in their improvement. The effects of these steps could be grouped according to the type of treatment undertaken. Dry matter content, pH and treatment intensity at each step contributed about 30% of the variability in functional properties due to processing steps. Relationships between the modifications of egg white functional properties and protein conformation were established. Between 46 and 78% of the variability in functional properties can be explained by protein denaturation, temperature and enthalpy changes. Copyright © 2004 Society of Chemical Industry
Clinically significant but unsuspected restless legs syndrome in patients with sleep apnea
Authors: Lakshminarayanan Sowmya , Paramasivan Kanchana Devi , Walters Arthur S. , Wagner Mary L. , Patel Shivani , Passi Vandna 
Published: December 2004

AbstractIn this prospective study, the prevalence of clinically significant restless legs syndrome (RLS) with symptoms at least 2 to 3 days per week was 8.3% in 60 sequentially polysomnographically studied patients with clinically significant sleep apnea (Apnea Index score > 5 or Respiratory Disturbance Index score > 10). Age‐matched spouses were used as a control group and showed a comparable prevalence of RLS at 2.5% (not significant). Although RLS appears to be only slightly more common in sleep apnea patients than in controls, the importance of this study lies in the fact that clinically significant RLS occurred in 1 of every 12 patients with sleep apnea and, in every case, the RLS was unsuspected before polysomnography. We recommend that all patients undergoing polysomnography to rule out sleep apnea be screened for the symptoms of RLS. We have found the MEMO–NIH consensus conference questionnaire administered at the time of polysomnography to be useful in this regard. © 2004 Movement Disorder Society
Elastic potentials at corners in Sobolev spaces with asymptotics
Authors: Kapanadze David 
Published: December 2004

AbstractThis paper is aimed at studying the single and double layer potentials related to the boundary value problems of elasticity theory for anisotropic case for the plane, corner domains. We start from the systems of second order elliptic differential equations with constant coefficients, write the fundamental solution and form the single and double layer (elastic) potentials. Applying the pseudo‐differential calculus we obtain the continuity results of the elastic potentials at corners in cone Sobolev spaces without and with asymptotics and characterize asymptotics of solutions. Copyright © 2004 John Wiley & Sons, Ltd.
Diblock and triblock copolymers of styrene and acetoxymethylstyrene by one‐pot ATRP
Authors: Rajan M. , Agarwal U. S. , Bailly C. , George K. E. , Lemstra P. J. 
Published: December 2004

AbstractWe describe the synthesis of diblock and triblock copolymers by sequential atom transfer radical polymerization of styrene and acetoxymethylstyrene. Contrary to the usual block copolymerization involving isolation of the macroinitiator, a convenient one‐pot procedure is developed. This is possible because of the preferential polymerization of acetoxymethylstyrene, even in the presence of residual styrene, as inferred from characterization of the intermediate polystyrenes and the block copolymers by size exclusion chromatography,1H NMR, Fourier transform infrared spectroscopy, differential scanning calorimetry, and GPEC techniques. The latent acetoxy functionalities in these block copolymers are shown to be easily unmasked to OH and Br functionalities, with the potential for block ionomers and dense graft architectures. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 575–583, 2005
The expression and regulation of ADAMTS‐1, ‐4, ‐5, ‐9, and ‐15, and TIMP‐3 by TGFβ1 in prostate cells: relevance to the accumulation of versican
Authors: Cross N.A. , Chandrasekharan S. , Jokonya N. , Fowles A. , Hamdy F.C. , Buttle D.J. , Eaton C.L. 
Published: December 2004

AbstractBACKGROUNDBenign prostatic hyperplasia (BPH) is characterized by a proportional increase in the size of the stromal compartment of the gland, involving alterations to extracellular matrix (ECM) components. Some of these changes have been associated with the activity and expression of transforming growth factor beta1 (TGFβ1). Versican (chondroitin sulphate proteoglycan‐2) is overexpressed in BPH and prostate cancer and potentially contributes to disease pathology. A sub‐group of the ADAMTS lineage of metalloproteases possess versican‐degrading properties and are potential regulators of proteoglycan accumulation associated with BPH. These enzymes have one major inhibitor in the ECM, tissue inhibitor of metalloproteinases (TIMP)‐3.METHODSThe effect of TGFβ on mRNA expression in prostatic stromal cells was determined by real‐time qRT‐PCR using primers to ADAMTS‐1, ‐4, ‐5, ‐9, ‐15, versican, and TIMP‐3. MMP‐inhibitory potential (TIMP activity) of conditioned medium was measured using a fluorometric peptide substrate.RESULTSProstatic stromal cell cultures consistently expressed ADAMTS‐1, ‐4, ‐5, ‐9, ‐15 and TIMP‐3, in contrast to PC3, DU145, and LNCaP cells which failed to express at least two ADAMTS transcripts. In stromal cells, TGFβ1 decreased ADAMTS‐1, ‐5, ‐9, and ‐15 transcripts and increased ADAMTS‐4, versican, and TIMP‐3. TGFβ also increased TIMP activity in conditioned medium.CONCLUSIONSThe induction of versican expression by TGFβ in BPH stromal cells is in agreement with histological studies. The negative effect of TGFβ1 on ADAMTS‐1, ‐5, ‐9, and ‐15 coupled with increases in their inhibitor, TIMP‐3 may aid the accumulation of versican in the stromal compartment of the prostate in BPH and prostate cancer. © 2004 Wiley‐Liss, Inc.
Description of the international consortium for prostate cancer genetics, and failure to replicate linkage of hereditary prostate cancer to 20q13
Authors: Schaid Daniel J. , Chang Bao Li ,   The International Consortium for Prostate Cancer Genetics
Published: December 2004

AbstractThe International Consortium for Prostate Cancer Genetics (ICPCG) is an international collaborative effort to pool pedigrees with hereditary prostate cancer (PC) in order to replicate linkage findings for PC. A strength of the ICPCG is the large number of well‐characterized pedigrees, allowing linkage analyses within large subsets. Given the heterogeneity and complexity of PC, the historical difficulties of synthesizing different studies reporting positive and negative linkage replication, and the use of different statistical analysis methods and different stratification criteria, the ICPCG provides a valuable resource to evaluate linkage for hereditary PC. To date, linkage of chromosome 20 (HPC20) to hereditary PC has been one of the strongest linkage signals, yet the efforts to replicate this linkage have been limited. This paper reports a linkage analysis of chromosome 20 markers for 1,234 pedigrees with multiple cases of PC ascertained through the ICPCG, and represents the most thorough attempt to confirm or refute linkage to chromosome 20. From the original 158 Mayo pedigrees in which linkage was detected, the maximum heterogeneity LOD (HLOD) score, under a recessive model, was 2.78. In contrast, for the 1,076 pedigrees not included in the original study, the maximum HLOD score (recessive model) was 0.06. Although, a few small linkage signals for chromosome 20 were found in various strata of this pooled analysis, this large study failed to replicate linkage to HPC20. This study illustrates the value of the ICPCG family collection to evaluate reported linkage signals and suggests that the HPC20 region does not make a major contribution to PC susceptibility. © 2004 Wiley‐Liss, Inc.
Studies on the bioactivity and elementology of marine algae from the coast of Karachi, Pakistan
Authors: Rizvi Muhammad Afzal , Shameel Mustafa 
Published: December 2004

AbstractSome species of marine benthic algae belonging to the Chlorophyta, Phaeophyta and Rhodophyta collected from different coastal areas of Karachi (Pakistan) were investigated for their antibacterial, antifungal, phytotoxic and insecticidal activities. Brown seaweeds showed greater antibacterial activity than the green and red ones. Botryocladia leptopoda (J. Ag.) Kylin exhibited the greatest antifungal activity and Codium shameelii Nizam. the least. The highest phytotoxic activity (95%) was displayed by Enteromorpha intestinalis (L.) Nees at 100 µg/mL concentration, while Osmundea pinnatiﬁda (Huds.) Stack. showed the low insecticidal activity as compared to the other investigated species. Many elements e.g. Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Na, Pb, Zn were determined quantitatively. Among them Ca, Cr and Pb were found to occur in largest amount in green seaweeds, Co, Cu, Fe and Zn in greatest quantity in brown seaweeds, while Cd, K, Mg and Na in highest proportion in the investigated red seaweeds. Copyright © 2004 John Wiley & Sons, Ltd.
The interaction of selected natural products with human recombinant glutathione transferases
Authors: Hayeshi Rose , Mukanganyama Stanley , Hazra Banasri , Abegaz Berhanu , Hasler Julia 
Published: December 2004

AbstractThe interaction of geshoidin, diospyrin and ergothioneine, with heterologously expressed human glutathione transferases (GSTs) was investigated in vitro. Diospyrin and geshoidin inhibited the three GST isoforms tested, with IC50 values in the range 0.1–0.5 µm, whereas ergothioneine had no effect on the GSTs. The predominant mode of inhibition was noncompetitive with respect to both glutathione (GSH) and 1‐chloro‐2,4‐dinitrobenzene (CDNB). Diospyrin, however, competitively inhibited A1‐1 and M1‐1 with respect to GSH and geshoidin displayed mixed inhibition toward A1‐1 with respect to GSH. The Ki values for diospyrin with respect to both GSH and CDNB were in the range 0.08–0.6 µM and those for geshoidin were in the range 16–173 µM. These results indicate that diospyrin is a potent inhibitor of heterologously expressed human GSTs A1‐1, M1‐1 and P1‐1. Diospyrin and geshoidin were also found to inactivate P1‐1 with diospyrin being a potent inactivator. Given these inhibitory properties, diospyrin may be a potential GST chemomodulator. Ergothioneine inactivated P1‐1 only after preincubation and it enhanced ethacrynic acid inactivation of P1‐1. Inactivation of P1‐1 by ergothioneine may have implications for the antioxidant roles of P1‐1 and ergothioneine in vivo. Copyright © 2004 John Wiley & Sons, Ltd.
Anticonﬂict actions of aqueous extracts of flowers of Achillea millefolium L. vary according to the estrous cycle phases in Wistar rats
Authors: Molina‐Hernandez M. , Tellez‐Alcantara N. P. , Diaz M. A. , Garcia J. Perez , Lopez J. I. Olivera , Jaramillo M. Teresa 
Published: December 2004

AbstractAnticonﬂict‐like effects of aqueous extract of ﬂowers of Achillea millefolium L., a plant with purported anxiolytic actions, were studied in female Wistar rats during late proestrus or diestrus. During late proestrus (p < 0.05), control rats displayed reduced conﬂict behavior compared with diestrus. Diazepam (2.0 mg/kg; i.p.) reduced conﬂict behavior both during late proestrus (p < 0.05) or diestrus (p < 0.05). Doses of 8.0 mg/kg (p < 0.05), 10.0 mg/kg (p < 0.05) or 12.0 mg/kg (p < 0.05) of Achillea millefolium reduced conﬂict behavior during late proestrus. Conversely, during diestrus, only the dose of 12.0 mg/kg (p < 0.05) of Achillea millefolium L. reduced conﬂict behavior. In conclusion, the anticonﬂict‐like actions of Achillea millefolium L. may vary according to the estrous cycle phase. Copyright © 2004 John Wiley & Sons, Ltd.
Effects of green tea catechin‐induced lipolysis on cytosol glycerol content in differentiated 3T3‐L1 cells
Authors: Mochizuki Miyako , Hasegawa Noboru 
Published: December 2004

AbstractThe inﬂuence of catechins in green tea on lipolysis in fully differentiated 3T3‐L1 cells was studied and glycerol release into the buffer, cytosol and residual triglyceride were measured.The addition of (‐)‐epigallocatechin‐3‐gallate (EGCG) stimulated glycerol release into the cytosol signiﬁcantly after incubation for 4 h, but had no effect on that into the buffer. However, (+)‐catechins did not produce a signiﬁcant increase in lipolysis. These data suggested that EGCG has strong lipolytic activity. Copyright © 2004 John Wiley & Sons, Ltd.
Effect of essential oil concentration on the pH of nutrient and Iso‐sensitest broth
Authors: Hood J. R. , Cavanagh H. M. A. , Wilkinson J. M. 
Published: December 2004

AbstractThe role of pH on the antimicrobial activity of essential oils has not been well studied. The effect of four essential oils: Backhousia citriodora, Melaleuca alternifolia, Lavandula angustifolia and Santalum spicatum (0.1% to 10%) on the pH of two commonly used media, nutrient broth and Iso‐sensitest broth, was therefore undertaken. Small (less than 0.5 pH units) but statistically signiﬁcant differences between the pH of the two media followed the addition of M. alternifolia, L. angustifolia and S. spicatum essential oil. In general the effect on pH was greatest at higher concentrations and the fall in pH was greatest in the nutrient broth. The addition of B. citriodora essential oil to nutrient broth resulted in a fall in pH from 7.29 ± 0.02 (no oil) to 5.2 ± 0.03 (10% oil). This effect was not observed in the Iso‐sensitest broth. Copyright © 2004 John Wiley & Sons, Ltd.
Yeast hydrolysate reduces body fat of dietary obese rats
Authors: Kim K. M. , Chang U. J. , Kang D. H. , Kim J. M. , Choi Y. M. , Suh H. J. 
Published: December 2004

AbstractThe purpose of this study was to assess the antiobesity effect of the yeast hydrolysate (DNF) on the body weight, body fat and plasma lipids levels of high‐fat fed rats. The weight gain of the HF (high fat diet) (162.58 ± 6.68 g) was signiﬁcantly (p < 0.05) greater than that of DNF‐1, DNF‐2, (high fat diet with DNF of 0.5 and 1.0 g/kg body weight, respectively) and control groups (143.19 ± 7.33 g, 139.20 ± 8.36 g, 130.23 ± 8.02 g, respectively). The wet weight of the epididymal fat and the perirenal fat pads of the DNF‐1, DNF‐2 and control groups were reduced signiﬁcantly (p < 0.05). A signiﬁcant (p < 0.05) increase of HDL‐cholesterol level of the DNF‐2 and control groups was observed. However, there was no signiﬁcant difference between DNF‐1 and DNF‐2. It was also found that the triacylglycerol (TG) levels decreased signiﬁcantly (p < 0.05) in the DNF‐2 group from that of the HF, but there was no signiﬁcant (p < 0.05) difference between DNF‐1 and DNF‐2. Copyright © 2004 John Wiley & Sons, Ltd.
Estragole‐induced behavioural changes in rats
Authors: Cosentino Rosana M. , Norte Maria C. B. , Lazarini Carlos A. 
Published: December 2004

AbstractCroton zehntneri is a popular plant used in the Brazilian folk medicine. Recently, the use of its essential oil showed depressive activity on the central nervous system (CNS). Chemical studies show that estragole (ES) is one of the main compounds of this oil. This work seeks to evaluate the activity of ES on behaviour models of depression and anxiety, in rats. Male rats (60 days) were distributed to four groups (n = 10) and treated with 1.0, 3.0 and 10.0 µL/100 g body weight, v.o. of ES (experimental) and saline (control). Forced swimming and open‐ﬁeld parameters were unaffected by the ES treatment suggesting no alterations on depression behaviour. A decrease was observed in the total time of social interaction in the 10.0 µL/100 g group in relation to other groups. This group showed a reduction in the total entries frequency on the elevated plus‐maze without differences to the other parameters of this test. No differences in holeboard parameters were observed suggesting no alterations in exploratory activity. These data suggest that administration of ES induced alterations in the CNS related to anxiety behaviour, expressed by the shortest total time in the social interaction model. Exposure to ES was capable of altering the motor activity in the plus‐maze test. These data are antagonistic to those observed with the Croton zehntneri essential oil in these models. Copyright © 2004 John Wiley & Sons, Ltd.
Antiulcer activity of methanol fraction of Barleria lupulina Lindl. in animal models
Authors: Suba V. , Murugesan T. , Pal M. , Mandal Subhash C. , Saha B. P. 
Published: December 2004

AbstractThe objective of the present study was to evaluate the gastric cytoprotective activity of the methanol extract of aerial parts of the plant Barleria lupulina Lindl (Acanthaceae) in albino rats using various models of ulcers such as drug induced ulcers, restraint ulcers, duodenal ulcers and pylorus ligated ulcers. The effect of the extract on gastric secretion and lipid peroxidation (thiobarbituric acid reacting substances TBARS) was also studied in rats. The extract at the tested dose of 200 mg/kg signiﬁcantly reduced the volume of gastric juice, total acidity and the ulcer index in pylorus ligated rats. It also afforded signiﬁcant protection against alcohol and indomethacin induced ulcer as well as stress induced ulceration. TBARS in the stomach of indomethacin treated rats was also reduced. In addition, it gave protection against duodenal ulcers. The study suggests that the methanol extract of aerial parts of Barleria lupulina Lindl. has a protective effect against experimental gastric and duodenal ulcers. Copyright © 2004 John Wiley & Sons, Ltd.
Antiinﬂammatory activity of Synurus deltoides
Authors: Park Joon Hyun , Son Kun Ho , Kim Sung Wan , Chang Hyeun Wook , Bae KiHwan , Kang Sam Sik , Kim Hyun Pyo 
Published: December 2004

AbstractSynurus deltoides Aiton, Nakai, is an edible plant that has been used as a folk medicine for treating inﬂammatory disorders. This investigation was carried out to establish the antiinﬂammatory activity of this plant material using a 75% ethanol extract from the aerial part of S. deltoides. Against the acute inﬂammatory animal model of mouse croton oil‐induced ear oedema assay, the extract did not show signiﬁcant inhibition at 100–800 mg/kg by oral administration. On the other hand, the extract showed considerable inhibition against the chronic inﬂammatory animal model of rat adjuvant‐induced arthritis (25% inhibition at 100 mg/kg/day) while prednisolone exerted 40% inhibition at 10 mg/kg/day. In addition, S. deltoides possessed strong analgesic activity (IC50 = 50 mg/kg) in the acetic acid‐induced writhing test. From the extract, ursolic acid and scopoletin were successfully isolated and their contents were found to be 0.31% and 0.37% (w/w), respectively, based on the dried extract by HPLC analysis. All the results obtained indicate that this plant material may be used beneﬁcially as an antiinﬂammatory agent having analgesic action. Copyright © 2004 John Wiley & Sons, Ltd.
Vibrational absorption spectra of CnS (n = 2, 6) and CnS2 (n = 7, 9, 11, 13, 15) linear carbon–sulfur clusters
Authors: Wang Haiyan , Szczepanski Jan , Cooke Andrew , Brucat Philip , Vala Martin 
Published: December 2004

AbstractCarbon–sulfur clusters have been generated by the laser ablation of mixed sulfur–graphite pellets and the pulsed‐jet discharge of acetylene/carbon disulfide/argon or diacetylene/carbon disulfide/argon mixtures. Fourier transform infrared absorption spectroscopic measurements of cryogenic argon matrices, aided by Becke's three‐parameter exchange functional and the gradient‐corrected functional of Lee, Yang, and Paar (B3LYP/6‐311G*) calculations of the previous paper, has led to the identification of six heretofore unknown CnSm clusters: C6S, C7S2, C9S2, C11S2, C13S2, and C15S2. In addition, C2S has also been formed and bands at 1662.6 and 857.2 cm−1 unambiguously assigned to its C–C and C–S stretching modes, respectively. Using 12C/13C isotopic substitution and density functional theory calculations, bands at 1377.9, 1368.8, 1832.4, and 1796.9 cm−1 have been assigned to the ν4, ν7, ν10, and ν12 C–C stretching vibrations in linear C6S, C7S2, C13S2, and C15S2 clusters, respectively. From intensity correlation measurements, the 2056.7, 1938.2, and 1504.5 cm−1 bands have been attributed to the C7S2 (ν5, ν6), and C15S2 (ν13) clusters, respectively. A “coincidence plot” has been used to ascribe several additional bands to the C9S2 (ν7, ν8), and C11S2 (ν9) clusters. © 2004 Wiley Periodicals, Inc. Int J Quantum Chem, 2005
Evaluation of bielectronic integrals for 1s Slater orbitals by using averages
Authors: Pérez J. E. , Cesco J. C. , Taurian O. E. , Ortiz F. S. , Rosso A. E. , Denner C. C. , Giubergia G. O. 
Published: December 2004

AbstractA new approach for evaluating the four‐center bielectronic integrals (12|34), involving 1s Slater‐type orbitals, is presented. The method uses the multiplication theorem for Bessel functions. The bielectronic integral is expressed in terms of a finite sum of functions, and a scaling parameter is introduced. In the present work, the scaling parameter used is an average. The results show that the first term in the sum is always the principal contribution, and the remainder has a corrective character. The whole scheme and its numerical trend are understood on the basis of a theorem recently proved. © 2004 Wiley Periodicals, Inc. Int J Quantum Chem, 2005
Infrared spectra and dissociation pathways of the linear carbon–sulfur clusters CnS and SCnS (n ≤ 29): Theoretical calculations
Authors: Wang Haiyan , Szczepanski Jan , Brucat Philip , Vala Martin 
Published: December 2004

AbstractEnergies, structural parameters, and harmonic vibrational frequencies of the linear carbon–sulfur clusters, CnSm (n = 1–29, m = 1–2), have been computed using density functional theory (DFT) with the B3LYP exchange‐correlation functional and Pople's 6‐311G* basis set. The energies and equilibrium geometries have been compared to MP2 (Møller–Plesset second‐order perturbation) and CCSD(T) calculations for C2S, C6S, and C7S2, three carbon–sulfur clusters recently observed and reported for the first time in the following article. The vibrational frequencies, predicted using B3LYP/6‐311G*, agree satisfactorily with the observed infrared (IR) band frequencies, with only a 17 cm−1 standard deviation for all experimentally known carbon–sulfur cluster CC stretching modes. Dissociation energies calculated for different loss mechanisms indicate that carbon–sulfur clusters prefer to lose CS, in agreement with experimental observations. © 2004 Wiley Periodicals, Inc. Int J Quantum Chem, 2005
Summary of ISO/TC 201 standard: XXI. ISO 21270:2004—Surface chemical analysis—X‐ray photoelectron and Auger electron spectrometers—Linearity of intensity scale
Authors: Seah M. P. 
Published: December 2004

AbstractThis International Standard specifies two methods for determining the maximum count rate for an acceptable limit of divergence from linearity of the intensity scale of Auger and x‐ray photoelectron spectrometers. It also includes methods to correct for intensity non‐linearities so that a higher maximum count rate can be employed for those spectrometers for which the relevant correction equations have been shown to be valid. Copyright © 2004 John Wiley & Sons, Ltd.