bluuebunny/crossref_metadata_2025_split huggingface dataset:
Defeat at Home? Issue-Ownership and Social Democratic Support in Scandinavia
Authors: Blomqvist Paula , Green-Pedersen Christoffer 
Published: September 2004

AbstractThe aim of this article is to account for the differences in electoral support for social democratic parties in Scandinavia in recent years. The main argument put forward is that the relative success of the Swedish Social Democratic Party (SAP) in preserving voter support compared to the major decline for both the Danish and Norwegian social democrats should be understood by focusing on two factors, both related to the phenomenon of issue-voting. We argue that the relative success of the SAP must been seen in light of the way in which traditional political issues, like employment and social welfare, have continued to dominate Swedish political debates, whereas in Norway and Denmark, new political issues, particularly immigration, have sailed up the political agenda and paved the way for new right-wing parties which attract social democratic voters. Secondly, we believe that one issue in particular, that of the future of the welfare state, is important for preserving social democratic support. Therefore, it is also relevant that the Swedish Social Democratic Party appears to have been more successful than social democratic parties in the neighbouring countries in convincing voters that it is the party best suited to preserve the existing welfare system.
Being Muslim: Islam, Islamism and Identity Politics
Authors: Ismail Salwa 
Published: September 2004

AbstractThe paper explores the construction of both Muslim and Islamist identities and the ways in which they interact, converge and diverge. This exploration is set against the background of debates on the nature of Islamism and its positioning vis-à-vis modernity and post-modernity. The paper argues that processes of modernity and post-modernity may be at work in the production of Muslim identities, but highlights the need to examine how different dimensions of identity formation such as socio-economic position, gender, age and lifestyle enter into the formation of Muslim selves. This is made necessary if we accept the premise of the sociality and historicity of religion.
Brood size reduction in Nicrophorus vespilloides after usurpation of carrion from Nicrophorus quadripunctatus (Coleoptera: Silphidae)
Authors: SUZUKI Seizi 
Published: September 2004

AbstractBurying beetles bury small vertebrate carcasses, which become food for their larvae. They sometimes usurp carcasses occupied and buried by other beetles. Brood sizes of intraspecific and interspecific intruders were examined using Nicrophorus quadripunctatus as the resident. The brood sizes of usurpers were not reduced relative to control brood sizes when the usurper was conspecific, but were reduced when the usurper was heterospecific (N. vespilloides).
Epizootics and behavioral alteration in the arctiid caterpillar Chionarctia nivea (Lepidoptera: Arctiidae) caused by an entomopathogenic fungus, Entomophaga aulicae (Zygomycetes: Entomophthorales)
Authors: YAMAZAKI Kazuo , SUGIURA Shinji , FUKASAWA Yu 
Published: September 2004

AbstractWe observed epizootics and behavioral alteration in the arctiid caterpillar Chionarctia nivea after infection by an entomopathogenic fungus, Entomophaga aulicae, in April at a riverbank in Kyoto Prefecture, central Japan. The density of arctiid cadavers infected with E. aulicae was 1.31 individuals/m2. The critically ill caterpillars crawled up the dead stems of grasses and herbs such as the common reed Phragmites communis and the Japanese mugwort Artemisia indica var. maximowiczii to die at and near the highest parts of the stems, while the healthy larvae usually wandered on the ground and fed on the leaves of small herbs. This behavioral difference could be caused by infection with E. aulicae to enhance dispersal of conidia by active discharge in Entomophthorales with the aid of wind and rainfall.
Species specificity in photoperiodic control of nymphal development in four species of cricket from north‐west China
Authors: ARAI Tetsuo , LIU Ju‐Peng , HUANG Chun‐Mei , CHENG Xin‐Yue , WATARI Yasuhiko , TAKEDA Makio 
Published: September 2004

AbstractPhotoperiodic regulation of nymphal development was examined in four species of cricket collected in the Xinjiang‐Uygur Autonomy Region, China (approximately 43°N, 81–89°E). Fifty percent of individuals of Modicogryllus frontalis reared at 28°C reached adulthood in approximately 80 days in conditions of 11 h light : 13 h dark (LD 11:13) to 14:10, and in approximately 95 days under LD 15:9 to 16:8. Melanogryllus desertus started adult emergence earliest under LD 16:8 at 28°C, but some individuals required much longer to mature, and thus two peaks of adult emergence occurred at approximately 60 and 160 days after hatching. More individuals hatched during the late peak in LD 18:6 than in LD 16:8. The mean nymphal period was approximately 100 days in LD 11:13 to 14:10. Both species showed short‐day type photoperiodic responses, but Mo. frontalis developed faster than did Me. desertus. The latter occupied a wider range of habitat conditions and was more variable in life cycle than the former. Modicogryllus burdigalensis started adult emergence earliest in LD 16:8 at 28°C in the six photoperiods tested, most adults emerging within 60 days. The mean nymphal period was 80 days in LD 15:9, 135 days in LD 14:10 and 80–100 days in LD 11:13 to 13:11, showing an intermediate type of photoperiodic response. Acheta domesticus was a long‐day type species, and the proportion of delayed individuals increased with decreasing photoperiod. In the dry climate of Xinjiang‐Uygur, nymphal overwintering seems to be successful in all of the four different patterns of nymphal development.
Redescription of two East Asian species of the tribe Epeolini (Hymenoptera: Apidae; Nomadinae)
Authors: RIGHTMYER Molly G. 
Published: September 2004

AbstractThe two Asian species supposedly belonging to the genus or subgenus Triepeolus Robertson, Epeolus tsushimensis Cockerell and Triepeolus ventralis (Meade‐Waldo), are redescribed and illustrated. Epeolus tsushimensis is confirmed to be a member of the genus Epeolus on the basis of the male genitalia and internal sterna.
Biology of Anagrus atomus (Hymenoptera: Mymaridae), an egg parasitoid of the grape leafhopper Arboridia kermanshah (Homoptera: Cicadellidae)
Authors: HESAMI Shahram , SEYEDOLESLAMI Hossein , EBADI Rahim 
Published: September 2004

AbstractBiology, morphology and oviposition behavior of Anagrus atomus (Linnaeus), an egg parasitoid of the grape leafhopper Arboridia kermanshah Dlabola in Isfahan, Iran, were investigated. Adults were smaller than those so far reported from other regions. Females continuously drummed on plant surfaces with their antennae to search for host eggs. Parasitoid eggs hatched 2–3 days after oviposition, and A. atomus had two larval instars. First instar larvae were sacciform and immobile. Second instar larvae appeared 4 days after oviposition and were very active, and doubled their body length. The prepupal and pupal stages lasted for 1 and 5–6 days, respectively. Adult emergence began 16 days after oviposition, and peaked on day 17.
Genus Pagastia Oliver (Diptera: Chironomidae) from Japan, with description of a new species
Authors: ENDO Kazuo 
Published: September 2004

AbstractA new species, Pagastia hidakamontana sp. nov., is described from the alpine zone of the Hidaka Mountains in Hokkaido, Japan. Pagastia orthogonia Oliver, so far known only from the Nearctic Region, is newly recorded from Japan and redescribed. Females of P. lanceolata (Tokunaga) and P. nivis (Tokunaga) are redescribed, and the synonymy of P. lanceolata with Syndiamesa (Lasiodiamesa) crassipilosa Tokunaga (= Pseudodiamesa crassipilosa) is proposed. Keys to males and females of Japanese species of the genus are provided.
VP7 Mediates the Interaction of Rotaviruses with Integrin αvβ3 through a Novel Integrin-Binding Site
Authors: Zárate Selene , Romero Pedro , Espinosa Rafaela , Arias Carlos F. , López Susana 
Published: September 2004

ABSTRACTRotavirus entry is a complex multistep process that depends on the trypsin cleavage of the virus spike protein VP4 into polypeptides VP5 and VP8 and on the interaction of these polypeptides and of VP7, the second viral surface protein, with several cell surface molecules, including integrin αvβ3. We characterized the effect of the trypsin cleavage of VP4 on the binding to MA104 cells of the sialic acid-dependent virus strain RRV and its sialic acid-independent variant, nar3. We found that, although the trypsin treatment did not affect the attachment of these viruses to the cell surface, their binding was qualitatively different. In contrast to the trypsin-treated viruses, which initially bound to the cell surface through VP4, the non-trypsin-treated variant nar3 bound to the cell through VP7. Amino acid sequence comparison of the surface proteins of rotavirus and hantavirus, both of which interact with integrin αvβ3 in an RGD-independent manner, identified a region shared by rotavirus VP7 and hantavirus G1G2 protein in which six of nine amino acids are identical. This region, which is highly conserved among the VP7 proteins of different rotavirus strains, mediates the binding of rotaviruses to integrin αvβ3 and probably represents a novel binding motif for this integrin.
cis
-Acting RNA Signals in the NS5B C-Terminal Coding Sequence of the Hepatitis C Virus Genome
Authors: Lee Haekyung , Shin Hyukwoo , Wimmer Eckard , Paul Aniko V. 
Published: September 2004

ABSTRACT

The
cis
-replicating RNA elements in the 5′ and 3′ nontranslated regions (NTRs) of the hepatitis C virus (HCV) genome have been thoroughly studied before. However, no
cis
-replicating elements have been identified in the coding sequences of the HCV polyprotein until very recently. The existence of highly conserved and stable stem-loop structures in the RNA polymerase NS5B coding sequence, however, has been previously predicted (A. Tuplin, J. Wood, D. J. Evans, A. H. Patel, and P. Simmonds, RNA 8:824-841, 2002). We have selected for our studies a 249-nt-long RNA segment in the C-terminal NS5B coding region (NS5BCR), which is predicted to form four stable stem-loop structures (SL-IV to SL-VII). By deletion and mutational analyses of the RNA structures, we have determined that two of the stem-loops (SL-V and SL-VI) are essential for replication of the HCV subgenomic replicon in Huh-7 cells. Mutations in the loop and the top of the stem of these RNA elements abolished replicon RNA synthesis but had no effect on translation. In vitro gel shift and filter-binding assays revealed that purified NS5B specifically binds to SL-V. The NS5B-RNA complexes were specifically competed away by unlabeled homologous RNA, to a small extent by 3′ NTR RNA, and only poorly by 5′ NTR RNA. The other two stem-loops (SL-IV and SL-VII) of the NS5BCR domain were found to be important but not essential for colony formation by the subgenomic replicon. The precise function(s) of these
cis
-acting RNA elements is not known.
LMP2A Does Not Require Palmitoylation To Localize to Buoyant Complexes or for Function
Authors: Katzman Rebecca B. , Longnecker Richard 
Published: September 2004

ABSTRACT
Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is expressed constitutively in lipid rafts in latently infected B lymphocytes. Lipid rafts are membrane microdomains enriched in cholesterol and sphingolipids selective for specific protein association. Lipid rafts have been shown to be necessary for B-cell receptor (BCR) signal transduction. LMP2A prevents BCR recruitment to lipid rafts, thereby abrogating BCR function. As LMP2A is palmitoylated, whether this fatty acid modification is necessary for LMP2A to localize to lipid rafts and for protein function was investigated. LMP2A palmitoylation was confirmed in latently infected B cells. LMP2A was found to be palmitoylated on multiple cysteines only by S acylation. An LMP2A mutant that was not palmitoylated was identified and functioned similar to wild-type LMP2A; unmodified LMP2A localized to lipid rafts, was tyrosine phosphorylated, was associated with LMP2A-associated proteins, was ubiquitinated, and was able to block calcium mobilization following BCR cross-linking. Therefore, palmitoylation of LMP2A is not required for LMP2A targeting to buoyant complexes or for function.
Early Bone Marrow Hematopoietic Defect in Simian/Human Immunodeficiency Virus C2/1-Infected Macaques and Relevance to Advance of Disease
Authors: Yamakami Kenji , Honda Mitsuo , Takei Masami , Ami Yasushi , Kitamura Noboru , Nishinarita Susumu , Sawada Shigemasa , Horie Takashi 
Published: September 2004

ABSTRACT

To clarify hematological abnormalities following infection with human immunodeficiency virus (HIV), we examined the hematopoietic capability of bone marrow by using cynomolgus monkeys infected with pathogenic simian/human immunodeficiency virus (SHIV) strain C2/1, an animal model of HIV infection. The relationship between the progress of the infection and the CD4/CD8 ratio of T lymphocytes or the amount of SHIV C2/1 viral load in the peripheral blood was also investigated. A colony assay was performed to assess the hematopoietic capability of bone marrow stem cells during the early and advanced phases of the infection. Colonies of granulocytes-macrophages (GM) were examined by PCR for the presence of the SIVmac239
gag
region to reveal direct viral infection. There was a remarkable decrease in the CFU-GM growth on days 1 and 3 postinoculation, followed by recovery on day 56. During the more advanced stage, the CFU-GM growth decreased again. There was minimal evidence of direct viral infection of pooled cultured CFU-GM despite the continuously low CD4/CD8 ratios. These results indicate that the decrease in colony formation by bone marrow stem cells is reversible and fluctuates with the advance of the disease. This decrease was not due to direct viral infection of CFU-GM. Our data may support the concept that, in the early phase, production of inhibitory factors or deficiency of a stimulatory cytokine is responsible for some of the bone marrow defects described in the SHIV C2/1 model.
Phosphatidylserine Is Not the Cell Surface Receptor for Vesicular Stomatitis Virus
Authors: Coil David A. , Miller A. Dusty 
Published: September 2004

ABSTRACT
The envelope protein from vesicular stomatitis virus (VSV) has become an important tool for gene transfer and gene therapy. It is widely used mainly because of its ability to mediate virus entry into all cell types tested to date. Consistent with the broad tropism of the virus, the receptor for VSV is thought to be a ubiquitous membrane lipid, phosphatidylserine (PS). However, the evidence for this hypothesis is indirect and incomplete. Here, we have examined the potential interaction of VSV and PS at the plasma membrane in more detail. Measurements of cell surface levels of PS show a wide range across cell types from different organisms. We demonstrate that there is no correlation between the cell surface PS levels and VSV infection or binding. We also demonstrate that an excess of annexin V, which binds specifically and tightly to PS, does not inhibit infection or binding by VSV. While the addition of PS to cells does allow increased virus entry, we show that this effect is not specific to the VSV envelope. We conclude that PS is not the cell surface receptor for VSV, although it may be involved in a postbinding step of virus entry.
mRNA Molecules Containing Murine Leukemia Virus Packaging Signals Are Encapsidated as Dimers
Authors: Hibbert Catherine S. , Mirro Jane , Rein Alan 
Published: September 2004

ABSTRACT

Prior work by others has shown that insertion of ψ (i.e., leader) sequences from the Moloney murine leukemia virus (MLV) genome into the 3′ untranslated region of a nonviral mRNA leads to the specific encapsidation of this RNA in MLV particles. We now report that these RNAs are, like genomic RNAs, encapsidated as dimers. These dimers have the same thermostability as MLV genomic RNA dimers; like them, these dimers are more stable if isolated from mature virions than from immature virions. We characterized encapsidated mRNAs containing deletions or truncations of MLV ψ or with ψ sequences from MLV-related acute transforming viruses. The results indicate that the dimeric linkage in genomic RNA can be completely attributed to the ψ region of the genome. While this conclusion agrees with earlier electron microscopic studies on mature MLV dimers, it is the first evidence as to the site of the linkage in immature dimers for any retrovirus. Since the Ψ
+
mRNA is not encapsidated as well as genomic RNA, it is only present in a minority of virions. The fact that it is nevertheless dimeric argues strongly that two of these molecules are packaged into particles together. We also found that the kissing loop is unnecessary for this coencapsidation or for the stability of mature dimers but makes a major contribution to the stability of immature dimers. Our results are consistent with the hypothesis that the packaging signal involves a dimeric structure in which the RNAs are joined by intermolecular interactions between GACG loops.
The DNA Element Controlling Expression of the Varicella-Zoster Virus Open Reading Frame 28 and 29 Genes Consists of Two Divergent Unidirectional Promoters Which Have a Common USF Site
Authors: Yang Min , Hay John , Ruyechan William T. 
Published: September 2004

ABSTRACT
The mechanism of the divergent expression of the varicella-zoster virus (VZV) ORF 28 and ORF 29 genes from a common intergenic DNA element, the ORF 28/29 promoter, is of interest based on the observation that both genes are expressed during VZV lytic infection but only the ORF 29 gene is expressed in latently infected neurons. In the work presented here, expression driven by the ORF 28/29 intergenic region was examined. We found that the promoter activity towards the ORF 29 direction is more responsive to activation by the major viral transactivator IE62 than that towards the ORF 28 direction in the context of our experimental system. Analysis of the functional DNA elements involved in IE62 activation of the bidirectional ORF 28/29 regulatory element revealed that in both transfected and VZV-superinfected cells it is a fusion of two unidirectional promoters overlapping an essential USF binding site but with distinct TATA elements. A single TATA element directs expression in the ORF 28 direction, whereas the two TATA elements directing ORF 29 gene expression are alternatively and differentially utilized for transcription initiation. We also identified an Sp1 site localized proximal to the ORF 28 gene which functions as an activator element for expression in both directions. These results indicate that the ORF 28 and ORF 29 genes can be expressed either coordinately or independently and that the observed expression of only the ORF 29 gene during VZV latency may involve neuron-specific cellular factors and/or structural aspects of the latent viral genome.
Influenza B Virus BM2 Protein Is a Crucial Component for Incorporation of Viral Ribonucleoprotein Complex into Virions during Virus Assembly
Authors: Imai Masaki , Watanabe Shinji , Ninomiya Ai , Obuchi Masatsugu , Odagiri Takato 
Published: September 2004

ABSTRACT

Influenza B virus contains four integral membrane proteins in its envelope. Of these, BM2 has recently been found to have ion channel activity and is considered to be a functional counterpart to influenza A virus M2, but the role of BM2 in the life cycle of influenza B virus remains unclear. In an effort to explore its function, a number of BM2 mutant viruses were generated by using a reverse genetics technique. The BM2ΔATG mutant virus synthesized BM2 at markedly lower levels but exhibited similar growth to wild-type (wt) virus. In contrast, the BM2 knockout virus, which did not produce BM2, did not grow substantially but was able to grow normally when BM2 was supplemented in
trans
by host cells expressing BM2. These results indicate that BM2 is a required component for the production of infectious viruses. In the one-step growth cycle, the BM2 knockout virus produced progeny viruses lacking viral ribonucleoprotein complex (vRNP). The inhibited incorporation of vRNP was regained by
trans
-supplementation of BM2. An immunofluorescence study of virus-infected cells revealed that distribution of hemagglutinin, nucleoprotein, and matrix (M1) protein of the BM2 knockout virus at the apical membrane did not differ from that of wt virus, whereas the sucrose gradient flotation assay revealed that the membrane association of M1 was greatly affected in the absence of BM2, resulting in a decrease of vRNP in membrane fractions. These results strongly suggest that BM2 functions to capture the M1-vRNP complex at the virion budding site during virus assembly.
Anti-Vpr Activity of a Yeast Chaperone Protein
Authors: Benko Zsigmond , Liang Dong , Agbottah Emmanuel , Hou Jason , Chiu Karen , Yu Min , Innis Scott , Reed Patrick , Kabat William , Elder Robert T. , Di Marzio Paola , Taricani Lorena , Ratner Lee , Young Paul G. , Bukrinsky Michael , Zhao Richard Yuqi 
Published: September 2004

ABSTRACT

Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during viral infection, including nuclear transport of the proviral integration complex, induction of cell cycle G
2
arrest, and cell death. In this report, we show that a fission yeast chaperone protein Hsp16 inhibits HIV-1 by suppressing these Vpr activities. This protein was identified through three independent genome-wide screens for multicopy suppressors of each of the three Vpr activities. Consistent with the properties of a heat shock protein, heat shock-induced elevation or overproduction of Hsp16 suppressed Vpr activities through direct protein-protein interaction. Even though Hsp16 shows a stronger suppressive effect on Vpr in fission yeast than in mammalian cells, similar effects were also observed in human cells when fission yeast
hsp16
was expressed either in
vpr
-expressing cells or during HIV-1 infection, indicating a possible highly conserved Vpr suppressing activity. Furthermore, stable expression of
hsp16
prior to HIV-1 infection inhibits viral replication in a Vpr-dependent manner. Together, these data suggest that Hsp16 inhibits HIV-1 by suppressing Vpr-specific activities. This finding could potentially provide a new approach to studying the contribution of Vpr to viral pathogenesis and to reducing Vpr-mediated detrimental effects in HIV-infected patients.