bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demographics, clinical findings and diagnoses of cranial thoracic myelopathies (T1–T6 vertebrae) in cats
Authors: Benito Benito Miguel , Lopes Bruno A , José-López Roberto , Ives Edward J , Gutierrez-Quintana Rodrigo , Freeman Paul , Sánchez-Masián Daniel 
Published: October 2023

Objectives The aim of the study was to describe the patient demographics, clinicopathological features and presumptive or final diagnoses in cats with myelopathies between the T1 and T6 vertebrae. Methods This retrospective multicentre case study enrolled cases between 2015 and 2022 that were diagnosed with myelopathies between the T1 and T6 vertebrae as the primary cause for the presenting clinical signs. Results A total of 21 cases matched the inclusion criteria, 13 males (11 castrated and 2 entire) and 8 spayed females (median age 93 months; range 5–192). Most of the cases presented with a chronic and progressive history (76% and 86%, respectively), with a median duration of 29 days (range 1–2880). At the time of presentation, 90% of the cases were localised to the T3–L3 spinal cord segments based on neurological examination. The most common underlying pathology was neoplasia (42.9%), followed by inflammatory (24%), anomalous (19%), degenerative (9.5%) and vascular (4.8%) disorders. The most common location was T3–T4 (29%), followed by T2–T3 and T5–T6 (19% each). The cutaneous trunci reflex was normal in 86% of the cases and most of the cases (71%) did not show spinal discomfort upon admission. Conclusions and relevance Neoplasia was the most common cause of cranial thoracic myelopathy in this study. The lack of pathognomonic clinical signs for this specific region highlights the importance of assessing the entire thoracolumbar region up to and including at least the T1 vertebra when investigating cases with signs consistent with a T3–L3 myelopathy.
Sensory modulation interventions for adults with mental illness: A scoping review
Authors: Kandlur Nayantara R , Fernandes Ashmica Claire , Gerard Suzanna Rupal , Rajiv Shruthi , Quadros Shalini 
Published: October 2023

Introduction Sensory Modulation Interventions (SMI) assist people with mental illnesses to regulate emotional and physiological arousal. We aimed to conduct a scoping review to identify the study designs, geographical distribution, modality/regimen, barriers and facilitators in using SMI for individuals with mental illnesses. Methods A systematic search was conducted using Arksey and O'Malley’s framework with studies published on SMI from January 2010 to November 2020. Results 17 articles were found to be relevant. One was a case-control study, qualitative ( N = 3), mixed-method ( N = 3), and case studies ( N = 3), and the rest were pilot interventional studies ( N = 8). The duration of sessions ranged from 2 to 30 minutes for 2 days to 3 years. The facilitators in using SMI were of being cost-effective and easily adaptable. Barriers were in terms of maintenance of sensory items. Sensory modulation based items related to proprioception, vestibular and tactile were the frequently used in the SMI based interventions used in the included studies. Conclusions SMI as an occupational therapy intervention for individuals with mental illnesses is evolving and more robust studies in the future are recommended.
Duration of dual-antiplatelet therapy after stent-assisted coil for unruptured intracranial aneurysm: A nationwide cohort study
Authors: Baik Minyoul , Jeon Jimin , Heo Seok-jae , Yoo Joonsang , Kim Jinkwon 
Published: October 2023

Background: Stent-assisted coil (SAC) is increasingly used to treat unruptured intracranial aneurysm (UIA). However, the optimal duration of dual-antiplatelet therapy (DAPT) after SAC insertion remains unknown. Aim: To assess the time-dependent effect of DAPT on the risk of ischemic and hemorrhagic complications after SAC. Methods: This is a retrospective cohort study among patients with UIA treated with SAC using the nationwide health claims database in South Korea between 2009 and 2020. Multivariate Cox regression analysis was used, which included the use of DAPT as a time-dependent variable. The effect of DAPT was investigated for each period of “within 90 days,” “91 to 180 days,” “181 to 365 days,” and “366 to 730 days” after SAC. The primary outcome was a composite of ischemic stroke and major bleeding in each period within two years after SAC. Results: Of the 15,918 patients, mean age at SAC was 57.6 ± 10.8 years, and 3815 (24.0%) were men. The proportion of patients on DAPT was 79.4% at 90 days, 58.3% at 180 days, and 28.9% at 1 year after SAC. During the 2 years after SAC, the primary composite outcome occurred in 356 patients (2.2%). DAPT significantly reduced the primary composite outcome within 90 days after SAC (adjusted hazard ratio (aHR), 0.44; 95% confidence interval (CI), 0.28–0.69; p < 0.001); however, this was not the case after 90 days (all p > 0.05). DAPT reduced ischemic stroke risk within 90 days (aHR, 0.31; 95% CI 0.18–0.54; p < 0.001), and 91 to 180 days after SAC (aHR 0.40; 95% CI 0.18–0.88; p = 0.022); however, after 180 days, DAPT was no longer beneficial. Conclusions: In patients with UIA treated with SAC, 3 months of DAPT was associated with a decreased risk of the composite of ischemic and hemorrhagic complications.
Routine health information system utilization and associated factors among health professionals in public health facilities in Dire Dawa, eastern Ethiopia: A cross-sectional study
Authors: Mekuria Samuel , Adem Hassen Abdi , Ayele Behailu Hawulte , Musa Ibsa , Enyew Daniel Berhanie 
Published: October 2023

Background Using reliable evidence from routine health information system (RHIS) over time is a vital aid to improve health outcome, tackling disparities, enhancing efficiency, and encouraging innovation. In Ethiopia, utilization of routine health data for improving the performance and quality of care was not well-studied in grassroot health facilities. Objective This study was conducted to determine the level of RHIS utilization and associated factors among health professionals in public health facilities of Dire Dawa, eastern Ethiopia. Methods An institution-based cross-sectional study was conducted among 378 health professionals from June 10 to July 20, 2020. Self-administered pretested-structured questionnaire was used to collect data from the participants. Data were entered using EpiData 3.1 and analyzed using Stata 16.0. Descriptive statistics was used to describe the basic characteristics of the participants, and multivariable logistic regression analysis was conducted to identify factors associated with RHIS utilization. Adjusted odds ratio (AOR) (95% CI) was used to report association and significance declared at a P-value <0.05. Results Good RHIS utilization among health professionals was 57.7% (95% CI: 52.6%, 62.6%). Good organizational support (AOR = 3.91, 95% CI: 2.01, 7.61), low perceived complexity of RHIS formats (AOR = 2.20, 95% CI: 1.23, 3.97), good self-efficacy (AOR = 2.52, 95% CI: 1.25, 5.10), and good decision-making autonomy (AOR = 3.97, 95% CI: 2.12, 7.43) were important factors associated with good RHIS utilization. Conclusions The level of good RHIS utilization among health professionals was low. Lack of self-confidence and empowerment, complexity of RHIS formats, and poor organizational support were significantly reducing RHIS utilization. Therefore, improving self-efficacy and decision-making capacity of health professionals through comprehensive training, empowerment, and organizational support would be essential.
Cut-off value of the eHEALS score as a measure of eHealth skills among rural residents in Gansu, China
Authors: Zhu Yang , Wang Xiaohui , You Xinyi , Zhao Hongmei , Guo Yawei , Cao Wangnan , Xin Meiqi , Li Jinghua 
Published: October 2023

Background In this study, our aim was to quantify eHealth literacy and determine a cut-off value for eHEALS scores that signifies adequate eHealth literacy among rural residents in Gansu, China. Methods This cross-sectional investigation encompassed 18 rural areas across three cities—Lanzhou, Wuwei, and Dingxi—in Gansu. A total of 451 residents were recruited and underwent evaluations for both eHealth literacy and eHealth skills, utilizing the eHEALS and self-constructed tasks, respectively. A receiving operator characteristic curve was plotted with eHealth skills as the dependent variable and eHealth literacy levels as the independent variable, aiming to determine a cut-off value for eHEALS indicating adequate eHealth literacy and evaluate its predictive capacity. Results Among the 451 respondents, 10.9% did not possess a personal electronic device with Internet access, while 6.4% owned but had never used them. Within the remaining 373 residents, the mean eHealth literacy score was 25.85 (SD:10.93), item scores ranged from 3.12 (SD:1.45) to 3.42 (SD:1.60). Completion rates for three eHealth skills varied from 39.1% to 59.8%. The cut-off value was 29.5 determined by the Youden index. The area under the receiver operating curve was 0.829, with a sensitivity of 86.7%, and a specificity of 66.8%. Conclusions Our findings emphasized that eHealth literacy among rural residents remains at a low level. Moreover, we identified a cut-off value of 29.5 for eHEALS scores that signifies adequate eHealth literacy within this demographic.
Patient-reported treatment outcomes and safety of direct-to-consumer teledermatology for finasteride treatment in male androgenetic alopecia: A cross-sectional study
Authors: von Büren Johannes , Hansen Inga , Kött Julian , Schröder Florian , Veneroso Juliana , Schneider Stefan W. , Abeck Finn 
Published: October 2023

Objective The use of direct-to-consumer (DTC) teledermatology platforms has increased, particularly for androgenetic alopecia (AGA). However, little is known about the efficacy and safety of these platforms. This study aimed to investigate the patient-reported treatment outcomes and safety of DTC teledermatology for the finasteride treatment of male AGA. Methods This retrospective, cross-sectional study used data from a German DTC platform for finasteride treatment between December 2021 and January 2023. Patient-reported outcomes were collected through voluntary follow-up questionnaires provided to the patients six weeks after the first prescription to assess treatment outcomes and safety. Results Data collection included 2269 patients. Of all patients who answered the follow-up questionnaire ( n = 191), 79% (150 out of 191) self-reported positive changes in hair appearance, and 59% (113 out of 191) reported an improvement in self-esteem under treatment. Patients with self-reported positive changes in hair appearance were more likely to report improved self-esteem ( P < 0.0001). Treatment-related adverse events occurred in 12% (22 out of 191) of the patients. Full treatment adherence was reported in 87% (167 out of 191) of patients. Conclusion From the patient's perspective, DTC teledermatology has the potential to improve hair appearance and self-esteem. Our results suggest that it may be an effective and safe treatment option for men with AGA, justifying low-threshold access. However, treatment-related adverse events should be closely monitored during follow-up. Further studies are required to evaluate the long-term effects of the DTC teledermatology treatment. By collecting real-world data, teledermatology platforms could be useful beyond their primary focus and could play an important role in the context of future research.
A positive association of serum CCN5/WISP2 levels with the risk of developing gestational diabetes mellitus: a case–control study
Authors: Hamdan Alshganbee Mohammed Farhan , Nabatchian Fariba , Farrokhi Vida , Fadaei Reza , Moradi Nariman , Afrisham Reza 
Published: October 2023

Abstract
Introduction
CCN5/WISP2 is prominently manifest in adipose tissue and has been linked to the pathogenesis of obesity, diabetes, and insulin resistance. However, discrepancies exist in previous studies, and little is known about its association with gestational diabetes mellitus (GDM). The current investigation is designed to examine the correlation of WISP2 with risk factors in GDM patients in comparison to healthy pregnant women for the first time.

Methods
This case–control study measured serum levels of CCN5, TNF-α, IL-6, adiponectin, and fasting insulin using ELISA kits in 88 GDM patients and 88 pregnant women.

Results
The GDM group had remarkably higher serum levels of CCN5 (379.41 ± 83.078 ng/ml) compared to controls (212.02 ± 77.935 ng/ml). In a similar vein, it was observed that patients diagnosed with GDM exhibited elevated levels of pro-inflammatory cytokines such as IL-6 and TNF-α; while conversely, adiponectin levels were found to be significantly lower than those observed in the control group (P < 0.0001). In women with GDM, a positive and significant correlation was observed between CCN5 and BMI, FBG, insulin, HOMA-IR, as well as IL-6 and TNF-α levels. In the adjusted model, the risk of GDM was significantly increased with elevated serum CCN5 level.

Conclusion
Our research indicates a noteworthy and affirmative correlation between the levels of CCN5 in the serum and the risk of developing GDM, along with its associated risk factors such as BMI, insulin resistance index, FBG, and inflammatory cytokines (TNF-α and IL-6). These findings suggest that CCN5 could potentially play a role in the etiology of GDM.
PRMxAI: protein arginine methylation sites prediction based on amino acid spatial distribution using explainable artificial intelligence
Authors: Khandelwal Monika , Rout Ranjeet Kumar 
Published: October 2023

Abstract
Background
Protein methylation, a post-translational modification, is crucial in regulating various cellular functions. Arginine methylation is required to understand crucial biochemical activities and biological functions, like gene regulation, signal transduction, etc. However, some experimental methods, including Chip–Chip, mass spectrometry, and methylation-specific antibodies, exist for the prediction of methylated proteins. These experimental methods are expensive and tedious. As a result, computational methods based on machine learning play an efficient role in predicting arginine methylation sites.

Results
In this research, a novel method called PRMxAI has been proposed to predict arginine methylation sites. The proposed PRMxAI extract sequence-based features, such as dipeptide composition, physicochemical properties, amino acid composition, and information theory-based features (Arimoto, Havrda-Charvat, Renyi, and Shannon entropy), to represent the protein sequences into numerical format. Various machine learning algorithms are implemented to select the better classifier, such as Decision trees, Naive Bayes, Random Forest, Support vector machines, and K-nearest neighbors. The random forest algorithm is selected as the underlying classifier for the PRMxAI model. The performance of PRMxAI is evaluated by employing 10-fold cross-validation, and it yields 87.17% and 90.40% accuracy on mono-methylarginine and di-methylarginine data sets, respectively. This research also examines the impact of various features on both data sets using explainable artificial intelligence.

Conclusions
The proposed PRMxAI shows the effectiveness of the features for predicting arginine methylation sites. Additionally, the SHapley Additive exPlanation method is used to interpret the predictive mechanism of the proposed model. The results indicate that the proposed PRMxAI model outperforms other state-of-the-art predictors.
Mabs, a suite of tools for gene-informed genome assembly
Authors: Schelkunov Mikhail I. 
Published: October 2023

Abstract
Background
Despite constantly improving genome sequencing methods, error-free eukaryotic genome assembly has not yet been achieved. Among other kinds of problems of eukaryotic genome assembly are so-called "haplotypic duplications", which may manifest themselves as cases of alleles being mistakenly assembled as paralogues. Haplotypic duplications are dangerous because they create illusions of gene family expansions and, thus, may lead scientists to incorrect conclusions about genome evolution and functioning.

Results
Here, I present Mabs, a suite of tools that serve as parameter optimizers of the popular genome assemblers Hifiasm and Flye. By optimizing the parameters of Hifiasm and Flye, Mabs tries to create genome assemblies with the genes assembled as accurately as possible. Tests on 6 eukaryotic genomes showed that in 6 out of 6 cases, Mabs created assemblies with more accurately assembled genes than those generated by Hifiasm and Flye when they were run with default parameters. When assemblies of Mabs, Hifiasm and Flye were postprocessed by a popular tool for haplotypic duplication removal, Purge_dups, genes were better assembled by Mabs in 5 out of 6 cases.

Conclusions
Mabs is useful for making high-quality genome assemblies. It is available at https://github.com/shelkmike/Mabs
The endometrial transcriptome transition preceding receptivity to embryo implantation in mice
Authors: Chan Hon Yeung , Tran Ha M. , Breen James , Schjenken John E. , Robertson Sarah A. 
Published: October 2023

Abstract
Background
Receptivity of the uterus is essential for embryo implantation and progression of mammalian pregnancy. Acquisition of receptivity involves major molecular and cellular changes in the endometrial lining of the uterus from a non-receptive state at ovulation, to a receptive state several days later. The precise molecular mechanisms underlying this transition and their upstream regulators remain to be fully characterized. Here, we aimed to generate a comprehensive profile of the endometrial transcriptome in the peri-ovulatory and peri-implantation states, to define the genes and gene pathways that are different between these states, and to identify new candidate upstream regulators of this transition, in the mouse.

Results
High throughput RNA-sequencing was utilized to identify genes and pathways expressed in the endometrium of female C57Bl/6 mice at estrus and on day 3.5 post-coitum (pc) after mating with BALB/c males (n = 3–4 biological replicates). Compared to the endometrium at estrus, 388 genes were considered differentially expressed in the endometrium on day 3.5 post-coitum. The transcriptional changes indicated substantial modulation of uterine immune and vascular systems during the pre-implantation phase, with the functional terms Angiogenesis, Chemotaxis, and Lymphangiogenesis predominating. Ingenuity Pathway Analysis software predicted the activation of several upstream regulators previously shown to be involved in the transition to receptivity including various cytokines, ovarian steroid hormones, prostaglandin E2, and vascular endothelial growth factor A. Our analysis also revealed four candidate upstream regulators that have not previously been implicated in the acquisition of uterine receptivity, with growth differentiation factor 2, lysine acetyltransferase 6 A, and N-6 adenine-specific DNA methyltransferase 1 predicted to be activated, and peptidylprolyl isomerase F predicted to be inhibited.

Conclusions
This study confirms that the transcriptome of a receptive uterus is vastly different to the non-receptive uterus and identifies several genes, regulatory pathways, and upstream drivers not previously associated with implantation. The findings will inform further research to investigate the molecular mechanisms of uterine receptivity.
Bcl-3 regulates T cell function through energy metabolism
Authors: Liu Hui , Zeng Lin , Pan Mengmeng , Huang Liwenhui , Li Hanying , Liu Mengxia , Niu Xinqing , Zhang Chenguang , Wang Hui 
Published: October 2023

Abstract
Background
Bcl-3 is a member of the IκB protein family and an essential modulator of NF-κB activity. It is well established that Bcl-3 is critical for the normal development, survival and differentiation of adaptive immune cells, especially T cells. However, the regulation of immune cell function by Bcl-3 through metabolic pathways has rarely been studied.

Results
In this study, we explored the role of Bcl-3 in the metabolism and function of T cells via the mTOR pathway. We verified that the proliferation of Bcl-3-deficient Jurkat T cells was inhibited, but their activation was promoted, and Bcl-3 depletion regulated cellular energy metabolism by reducing intracellular ATP and ROS production levels and mitochondrial membrane potential. Bcl-3 also regulates cellular energy metabolism in naive CD4+ T cells. In addition, the knockout of Bcl-3 altered the expression of mTOR, Akt, and Raptor, which are metabolism-related genes, in Jurkat cells.

Conclusions
This finding indicates that Bcl-3 may mediate the energy metabolism of T cells through the mTOR pathway, thereby affecting their function. Overall, we provide novel insights into the regulatory role of Bcl-3 in T-cell energy metabolism for the prevention and treatment of immune diseases.
Incidence of postoperative opioid-induced respiratory depression episodes in patients on room air or supplemental oxygen: a post-hoc analysis of the PRODIGY trial
Authors: Doufas Anthony G. , Laporta Mariana L. , Driver C. Noelle , Di Piazza Fabio , Scardapane Marco , Bergese Sergio D. , Urman Richard D. , Khanna Ashish K. , Weingarten Toby N. ,   The Prediction of Opioid-induced respiratory Depression In patients monitored by capnoGraphY (PRODIGY) Group Investigators, Jungquist Carla R. , Morimatsu Hiroshi , Uezono Shoichi , Lee Simon , Ti Lian Kah , McIntyre Robert , Tornero Carlos , Dahan Albert , Saager Leif , Wittmann Maria , Auckley Dennis , Brazzi Luca , Le Guen Morgan , Soto Roy , Schramm Frank , Buhre Wolfgang , Overdyk Frank J. 
Published: October 2023

Abstract
Background
Supplemental oxygen (SO) potentiates opioid-induced respiratory depression (OIRD) in experiments on healthy volunteers. Our objective was to examine the relationship between SO and OIRD in patients on surgical units.

Methods
This post-hoc analysis utilized a portion of the observational PRediction of Opioid-induced respiratory Depression In patients monitored by capnoGraphY (PRODIGY) trial dataset (202 patients, two trial sites), which involved blinded continuous pulse oximetry and capnography monitoring of postsurgical patients on surgical units. OIRD incidence was determined for patients receiving room air (RA), intermittent SO, or continuous SO. Generalized estimating equation (GEE) models, with a Poisson distribution, a log-link function and time of exposure as offset, were used to compare the incidence of OIRD when patients were receiving SO vs RA.

Results
Within the analysis cohort, 74 patients were always on RA, 88 on intermittent and 40 on continuous SO. Compared with when on RA, when receiving SO patients had a higher risk for all OIRD episodes (incidence rate ratio [IRR] 2.7, 95% confidence interval [CI] 1.4–5.1), apnea episodes (IRR 2.8, 95% CI 1.5–5.2), and bradypnea episodes (IRR 3.0, 95% CI 1.2–7.9). Patients with high or intermediate PRODIGY scores had higher IRRs of OIRD episodes when receiving SO, compared with RA (IRR 4.5, 95% CI 2.2–9.6 and IRR 2.3, 95% CI 1.1–4.9, for high and intermediate scores, respectively).

Conclusions
Despite oxygen desaturation events not differing between SO and RA, SO may clinically promote OIRD. Clinicians should be aware that postoperative patients receiving SO therapy remain at increased risk for apnea and bradypnea.

Trial registration
Clinicaltrials.gov: NCT02811302, registered June 23, 2016.