bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photo‐Compartmentalized Decellularized Matrix‐Hyaluronan Hybrid Units for Pancreatic Tumor‐Stroma Modeling
Authors: Monteiro Maria V. , Henriques‐Pereira Margarida , Neves Bruno M. , Duarte Iola D. , Gaspar Vítor M. , Mano João F. 
Published: October 2023

AbstractTissue‐derived decellularized extracellular matrix (dECM) provides unique biomolecular cues for bioengineering 3D tumor models with increasingly physiomimetic features. This biomimicry potential can be expanded by combining dECM with key components of native tissues, such as glycosaminoglycans (GAGs). Yet, this combination remains highly challenging, often requiring complex non‐natural chemical modifications of dECM biomaterials. Taking advantage of naturally occurring tyrosine moieties, herein a universally applicable methodology is proposed for generating dECM‐Hyaluronic acid (HA)‐tyramine hydrogels. Enriching dECM with HA, is particularly relevant for modeling tumor‐stroma interplay in vitro. By using visible light crosslinking, pancreatic tissue‐specific dECM‐HA‐Tyr tumor‐stroma compartmentalyzed platforms are rapidly fabricated on‐demand, in superhydrophobic surfaces, while avoiding complex dECM chemical modifications. The generated dECM‐HA‐Tyr spherically shaped models are able to mimic the desmoplastic stromal environment and are employed for dual screening of pancreatic anti‐stromal and anti‐tumoral therapeutics. The tumor‐stroma models display higher susceptibility to gemcitabine following a first treatment with anti‐stroma therapeutics. The developed photo‐compartmentalized models ultimately allow to decouple the tumor and stroma compartment, thus enabling the inclusion of other stromal/cancer cells. Moreover, the possibility of combining dECM with virtually any class of tyramine‐functionalized macromolecules via on‐demand photocrosslinking as herein proposed, extends the use of such hybrid dECM hydrogels.
Statically Very Hydrophilic but Dynamically Hydrophobic Surfaces Showing Surprising Water Sliding Performance
Authors: Nakamura Satoshi , Kakiuchida Hiroshi , Okada Masahisa , Hozumi Atsushi 
Published: October 2023

AbstractIt is generally believed that water droplets on (super)hydrophilic surfaces is spread and strongly pin to the surface. The surface chemistry that achieves the paradoxical surface properties of being statically very hydrophilic, but exhibiting outstanding water sliding properties, has not yet established. Here, a facile approach to prepare such unusual surfaces based on a combination of a sol–gel process using an organosilane containing polyethylene glycol units and tetraethoxysilane, and subsequent alkali‐treatment is reported. The resulting sol–gel thin films are smooth, transparent, hydrophilic (static contact angle (CA) (θS) of ≈37°) and show a liquid‐like nature with both low CA hysteresis (≈4°) and sliding angle (α) for 10 µL water droplets (≈10°). Surprisingly, after alkali‐treatment for sufficient time, the surface becomes very hydrophilic (θS of ≈14°) while improving its dynamic wetting behaviors (CA hysteresis of ≈3° and α for 10 µL water droplet of ≈4°), such that even a mere 0.5 µL water drop can slide down smoothly without pinning and/or tailing.
Ecofriendly Transfer Printing for Biodegradable Electronics Using Adhesion Controllable Self‐Assembled Monolayers
Authors: Lee Seung‐Min , Lee Woo‐Jin , Bae Jae‐Young , Gu Ji‐Woo , Lee Seunghwan , Yeo Ki Baek , Lee Jaewook , Kim Joon‐Woo , Lee Ju‐Yong , Kim Jeonghyun , Jang Hyejin , Jun Sang Ho , Kang Seung‐Kyun 
Published: October 2023

AbstractThe biodegradable electronics are on the rise, not just due to their role in medical implants, but also because of their eco‐friendly attributes. A variety of methods, including transfer printing, have been employed to integrate inorganic electronics onto biodegradable polymer substrates. However, the use of expensive materials, multiple intermediary steps, and labor‐intensive procedures can undermine their environment‐friendly benefits. Here, a straightforward yet efficient fabrication method is introduced for creating high‐performance biodegradable electronic devices. This method leverages the controlled adhesion between the biodegradable device and substrate using self‐assembled monolayers of octadecyltrichlorosilane. Mechanical and thermal analyses based on scratch tests and time‐domain thermoreflectance quantify the adhesion by adjusting the packing density of octadecyltrichlorosilane. Controlled adhesion allows the photolithography process without delamination while facilitating easy delamination during transfer printing. The authors demonstrate the direct fabrication of electronics consisted of inorganic materials (Mg, Zn, SiO2, Si nanomembrane) on wafers and transfer‐printing onto polymer substrates via a single transfer step. This streamlined approach enables wafer‐scale fabrication of biodegradable electronics, highlighting its potential for mass manufacturing. Pilot conceptual demonstration of mass‐produced edible hydration sensors and their application in salivation measurement through in vivo model show the potential capability of proposed fabrication method in the use of practical level.
Self‐Doping and Self‐Acid‐Doping of Conjugated Polymer Bioelectronics: The Case for Accuracy in Nomenclature
Authors: Fidanovski Kristina , Gu Modi , Travaglini Lorenzo , Lauto Antonio , Mawad Damia 
Published: October 2023

AbstractConjugated polymers are enabling the development of flexible bioelectronics, largely driven by their organic nature which facilitates modification and tuning to suit a variety of applications. As organic semiconductors, conjugated polymers require a dopant to exhibit electrical conductivity, which in physiological conditions can result in dopant loss and thereby deterioration in electronic properties. To overcome this challenge, “self‐doped” and self‐acid‐doped conjugated polymers having ionized pendant groups covalently bound to their backbone are being developed. The ionized group in a “self‐doped” polymer behaves as the counterion that maintains electroneutrality, while an external dopant is required to induce charge transfer. The ionized group in a self‐acid‐doped polymer induces charge transfer and behaves as the counterion balancing the charges. Despite their doping processes being different, the two terms, self‐doped and self‐acid‐doped, are often used interchangeably in the literature. Here, the differences are highlighted in the doping mechanisms of self‐doped and self‐acid‐doped polymers, and it is proposed that the term “self‐doped” should be replaced by “self‐compensated,” while reserving the term self‐acid‐doped for polymers that are intrinsically doped without the need of an external dopant. This is followed by a summary of examples of self‐acid‐doping in bioelectronics, highlighting their stability in the conductive state under physiological conditions.
Synthesis of α‐Quaternary Carboxylic Acids through Cu‐Catalyzed Hydrocarboxylation of Allenes with Diisobutylaluminum Hydride and Carbon Dioxide
Authors: Lee Sanghyun , Lee Yunmi 
Published: October 2023

AbstractA regioselective method for synthesizing β,γ‐unsaturated α‐quaternary carboxylic acids through the copper‐catalyzed hydrocarboxylation of allenes with CO2 is described. Allenes undergo hydroalumination with diisobutylaluminum hydride, followed by carboxylation of the resulting allylaluminum intermediate. The presence of an N‐heterocyclic carbene ligand is essential for enhancing the reactivity of the reaction. The good functional group compatibility of the reaction allows the synthesis of a wide range of functionalized α‐quaternary carboxylic acids with high regio‐ and stereoselectivities for synthetic and medicinal applications.
Rh‐catalyzed Asymmetric C(sp3)−H Arylation of 8‐Benzylquinolines with Arylboronic Acids
Authors: Zhang Zuo‐Yu , Gou Bo‐Bo , Wang Quannan , Gu Qing , You Shu‐Li 
Published: October 2023

AbstractRh‐catalyzed asymmetric C(sp3)−H arylation of 8‐benzylquinolines with arylboronic acids was developed. In the presence of 5 mol% BINOL‐derived chiral cyclopentadienyl rhodium complex, asymmetric benzylic C(sp3)−H bond arylation reaction proceeded smoothly to afford a series of enantioenriched triarylmethanes in moderate to good yields with good to excellent enantioselective control (24–89% yields, 63–93% ee). The method displays a broad substrate scope and good functional group tolerance under mild conditions.
In Vivo Behavior of Systemically Administered Encapsulin Protein Nanocages and Implications for their use in Targeted Drug Delivery
Authors: Rennie Claire , Sives Caitlin , Boyton India , Diaz Dennis , Gorrie Catherine , Vittorio Orazio , Collins‐Praino Lyndsey , Care Andrew 
Published: October 2023

AbstractEncapsulins, self‐assembling protein nanocages derived from prokaryotes, are promising nanoparticle‐based drug delivery systems (NDDS). However, the in vivo behavior and fate of encapsulins are poorly understood. In this study, the interactions between the model encapsulin from Thermotoga maritima (TmEnc) and key biological barriers encountered by NDDS are probed. Here, a purified TmEnc formulation that exhibits colloidal stability, storability, and blood compatibility is intravenously injected into BALB/c mice. TmEnc has an excellent nanosafety profile, with no abnormal weight loss or gross pathology observed, and only temporary alterations in toxicity biomarkers are detected. Notably, TmEnc demonstrates immunogenic properties, inducing the generation of nanocage‐specific IgM and IgG antibodies, but without any prolonged pro‐inflammatory effects. An absence of antibody cross‐reactivity also suggests immune‐orthogonality among encapsulin systems. Moreover, TmEnc forms a serum‐derived protein corona on its surface which changes dynamically and appears to play a role in immune recognition. TmEnc's biodistribution profile further reveals its sequestration from the blood circulation by the liver and then biodegrades within Kupffer cells, thus indicating clearance via the mononuclear phagocyte system. Collectively, these findings provide critical insights into how encapsulins behave in vivo, thereby informing their future design, modification, and application in targeted drug delivery.
Multi‐Scale Label‐Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two‐Photon Microscopy
Authors: Chang Shuaibin , Yang Jiarui , Novoseltseva Anna , Abdelhakeem Ayman , Hyman Mackenzie , Fu Xinlei , Li Chenglin , Chen Shih‐Chi , Augustinack Jean C. , Magnain Caroline , Fischl Bruce , Mckee Ann C. , Boas David A. , Chen Ichun Anderson , Wang Hui 
Published: October 2023

AbstractThe study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation‐free reconstruction. Here, the authors describe an integrated serial sectioning polarization‐sensitive optical coherence tomography (PSOCT) and two photon microscopy (2PM) system to provide label‐free multi‐contrast imaging of intact brain structures, including scattering, birefringence, and autofluorescence of human brain tissue. The authors demonstrate high‐throughput reconstruction of 4 × 4 × 2cm3 sample blocks and simple registration between PSOCT and 2PM images that enable comprehensive analysis of myelin content, vascular structure, and cellular information. The high‐resolution 2PM images provide microscopic validation and enrichment of the cellular information provided by the PSOCT optical properties on the same sample, revealing the densely packed fibers, capillaries, and lipofuscin‐filled cell bodies in the cortex and white matter. It is shown that the imaging system enables quantitative characterization of various pathological features in aging process, including myelin degradation, lipofuscin accumulation, and microvascular changes, which opens up numerous opportunities in the study of neurodegenerative diseases in the future.
Photodriven Methane Conversion on Transition Metal Oxide Catalyst: Recent Progress and Prospects
Authors: Wang Pu , Shi Run , Zhao Jiaqi , Zhang Tierui 
Published: October 2023

AbstractMethane as the main component in natural gas is a promising chemical raw material for synthesizing value‐added chemicals, but its harsh chemical conversion process often causes severe energy and environment concerns. Photocatalysis provides an attractive path to active and convert methane into various products under mild conditions with clean and sustainable solar energy, although many challenges remain at present. In this review, recent advances in photocatalytic methane conversion are systematically summarized. As the basis of methane conversion, the activation of methane is first elucidated from the structural basis and activation path of methane molecules. The study is committed to categorizing and elucidating the research progress and the laws of the intricate methane conversion reactions according to the target products, including photocatalytic methane partial oxidation, reforming, coupling, combustion, and functionalization. Advanced photocatalytic reactor designs are also designed to enrich the options and reliability of photocatalytic methane conversion performance evaluation. The challenges and prospects of photocatalytic methane conversion are also discussed, which in turn offers guidelines for methane‐conversion‐related photocatalyst exploration, reaction mechanism investigation, and advanced photoreactor design.
Cover crops impact phosphorus cycling and environmental efficiency in a corn–soybean system
Authors: Bourns Megan A. , Nelson Nathan O. , Carver R. Elliott , Roozeboom Kraig L. , Kluitenberg Gerard J. , Tomlinson Peter J. , Kang Qing , Hettiarachchi Ganga M. 
Published: October 2023

AbstractSolutions to simultaneously meet crop phosphorus (P) demand and reduce P loss from agricultural systems are required to improve the sustainability of our production. Our objective was to determine the effect of cover crop addition (with cover crop [CC], and without cover crop [NC]) and P fertilizer management (zero P control [CN], fall broadcast [FB], and spring injected [SI]) on P cycling, agronomic P use efficiency (PUE), and environmental P use efficiency (EPUE), measured as the percent of P lost in runoff water, in a no‐till corn (Zea mays)–soybean [Glycine max (L.) Merr.] rotation. The experiment was a 2 × 3 factorial. Both FB and SI increased P cycling through the main crop and cover crop tissue, compared to the CN. Cover crop did not affect main crop P uptake, removal in grain, or return to soil in crop residue, but CC treatment increased total P uptake and return in the system. Cover crop effects on P uptake, removal, and return were the same regardless of P fertilization treatments. Phosphorus fertilizer and CC treatments did not affect PUE. Both FB and SI P fertilizer management decreased EPUE, although SI tended to decrease EPUE less than FB. Cover crop significantly improved EPUE only in 2019, when CC reduced erosion losses. Therefore, cover crops were not consistently beneficial for reducing the environmental effects of P fertilization, and their use cannot replace the importance of sound P fertilizer stewardship, including sub‐surface P placement. However, cover crops play an important role in improving EPUE in years with high erosion loss potential.
High‐Pressure Fabrication of Binary Organic Solar Cells with High Molecular Weight D18 Yields Record 19.65 % Efficiency
Authors: Lu Hao , Liu Wenlong , Ran Guangliu , Liang Zezhou , Li Hongxiang , Wei Nan , Wu Hongbo , Ma Zaifei , Liu Yahui , Zhang Wenkai , Xu Xinjun , Bo Zhishan 
Published: October 2023

AbstractIn this work, inspired by the principles of a pressure cooker, we utilized a high‐pressure method to address the processing challenges associated with high molecular weight polymers. Through this approach, we successfully dissolved high molecular weight D18 in chloroform at 100 °C within a pressure‐tight vial. The increased steam pressure raised the boiling point and dissolving capacity of chloroform, enabling the creation of a hybrid film with superior properties, including more ordered molecular arrangement, increased crystallinity, extended exciton diffusion length, and improved phase morphology. Organic solar cells (OSCs) based on D18 : L8‐BO prepared using this high‐pressure method achieved an outstanding power conversion efficiency of 19.65 %, setting a new record for binary devices to date. Furthermore, this high‐pressure method was successfully applied to fabricate OSCs based on other common systems, leading to significant enhancements in device performance. In summary, this research introduces a universal method for processing high molecular weight D18 materials, ultimately resulting in the highest performance reported for binary organic solar cells.
A single 8‐hydroxyquinoline‐appended bile acid fluorescent probe obtained by click chemistry for solvent‐dependent and distinguishable sensing of zinc(II) and cadmium(II)
Authors: Zhang Zhen , Zou Yuan , Liu Jihua 
Published: October 2023

AbstractConstruction of fluorescent probes for zinc ion (Zn2+) and cadmium ion (Cd2+) is significant for the safety of humans. However, the discriminating recognition of Zn2+ and Cd2+ by a single probe remains challenging owing to their similar properties. Herein, a novel deoxycholic acid derivative containing 8‐hydroxyquinoline fluorophore has been facilely synthesized through click chemistry to form a clamp‐like probe. Using its perfect bonding cavity from 1,2,3‐triazole and quinoline, this molecule showed favorable solvent‐dependent fluorescent responses and distinguished Zn2+ and Cd2+ in different solvents. In ethanol aqueous solution, it displayed good selectivity and ratiometric fluorescence to Zn2+ with 30 nm spectroscopic red‐shifts. In acetonitrile aqueous solution, it exhibited good selectivity and ratiometric fluorescence to Cd2+ with 18 nm spectroscopic red‐shifts. Moreover, the unique microstructural features of the probe in assembly were used to reflect its recognition processes. Due to its merits of low detection limit and instant response time, the probe was utilized for sensing Zn2+ and Cd2+ in water, beer and urine with high accuracy. Meanwhile, this probe served as a handy tool and was employed to obtain inexpensive test strips for the prompt and semiqualitative analysis of Zn2+ and Cd2+ with the naked eye.
A novel screening method of DNA methylation biomarkers helps to improve the detection of colorectal cancer and precancerous lesions
Authors: Li Yuan , Li Bin , Jiang Rou , Liao Leen , Zheng Chunting , Yuan Jie , Zeng Liuhong , Hu Kunling , Zhang Yuyu , Mei Weijian , Hong Zhigang , Xiao Binyi , Kong Lingheng , Han Kai , Tang Jinghua , Jiang Wu , Pan Zhizhong , Zhang Shenyan , Ding Peirong 
Published: October 2023

AbstractBackgroundColorectal cancer (CRC) is one of the most common malignancies, and early detection plays a crucial role in enhancing curative outcomes. While colonoscopy is considered the gold standard for CRC diagnosis, noninvasive screening methods of DNA methylation biomarkers can improve the early detection of CRC and precancerous lesions.MethodsBioinformatics and machine learning methods were used to evaluate CRC‐related genes within the TCGA database. By identifying the overlapped genes, potential biomarkers were selected for further validation. Methylation‐specific PCR (MSP) was utilized to identify the associated genes as biomarkers. Subsequently, a real‐time PCR assay for detecting the presence of neoplasia or cancer of the colon or rectum was established. This screening approach involved the recruitment of 978 participants from five cohorts.ResultsThe genes with the highest specificity and sensitivity were Septin9, AXL4, and SDC2. A total of 940 participants were involved in the establishment of the final PCR system and the subsequent performance evaluation test. A multiplex TaqMan real‐time PCR system has been illustrated to greatly enhance the ability to detect precancerous lesions and achieved an accuracy of 87.8% (95% CI 82.9–91.5), a sensitivity of 82.7% (95% CI 71.8–90.1), and a specificity of 90.1% (95% CI 84.3–93.9). Moreover, the detection rate of precancerous lesions of this assay reached 55.0% (95% CI 38.7–70.4).ConclusionThe combined detection of the methylation status of SEPT9, SDC2, and ALX4 in plasma holds the potential to further enhance the sensitivity of CRC detection.
Distribution of regional lymph lode metastasis in unilateral nasopharyngeal carcinoma and the suggestions for selective prophylactic irradiation with intensity‐modulated radiotherapy
Authors: Lin Feifei , Qiu Zichen , Xie Dehuan , Zhou Xiong , Wang Lei , Wu Zheng , Cheng Wanqin , Lyu Shaowen , Su Yong , Tao Yalan 
Published: October 2023

AbstractPurposeTo address the regional lymph node (RLN) distribution and the long‐term efficacy in unilateral nasopharyngeal carcinoma (NPC), providing elective irradiation for RLN with intensity‐modulated radiotherapy (IMRT).MethodsThe involvement of clinical data of 136 patients with unilateral NPC, who underwent IMRT from November 2003 to December 2020 were analyzed retrospectively. The therapeutic effect and failure pattern of RLN metastasis were evaluated.ResultsOf 57.1% patients have bilateral RLN metastasis. The rate of contralateral RLNs metastasis is lower than that of ipsilateral RLNs. Contralateral RLN metastasis mainly occurs in level VIIa (39.0%) and II (38.2%). While level IVa is only 0.7%, and none of RLN metastasis was found in level IVb and Va. The median follow‐up was 70 months, and the 3‐, 5‐and 10‐year regional recurrence‐free survival (RRFS) were 94.1%, 93.1%, and 93.1%, respectively.ConclusionRoutine prophylactic irradiation may not include contralateral lower neck LN and level Va for N0‐1 unilateral NPC.
Green and Scalable Biopolymer‐Based Aqueous Polyelectrolyte Complexes for Zinc‐Ion Charge Storage Devices
Authors: Fernández‐Benito Amparo , Martinez‐López Juan Carlos , Javad Jafari Mohammad , Solin Niclas , Martinez Jose G. , Garcia‐Gimenez Daniel , Ederth Thomas , Inganäs Olle , Carretero‐González Javier 
Published: October 2023

AbstractGreen and scalable materials are essential to fulfill the need of electrification for transitioning into a fossil‐fuels free society, and sustainability is a requirement for all new technologies. Rechargeable batteries are one of the most important elements for electrification, enabling the transition to mobile electronics, electrical vehicles and grid storage. We here report synthesis and characterization of polyelectrolyte complexes of alginate and chitosan, both biopolymers deriving from the sea, for transport of zinc ions in hydrogel electrolytes. We have used vibrational spectroscopy, thermal measurements and microscopy, as well as transport measurements with ohmic or blocking contacts. The transference number for zinc ions is close to 1, the conductivity is ≈10 mS/cm, with stability at Zn interfaces seen through 7000 cycles in symmetric zinc//zinc cell.