bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of Molecular Targets and Mechanisms of Curcumin in the Treatment of
COVID-19 with Depression by an Integrative Pharmacology Strategy
Authors: Zhu Dongwei , Zhou Xianmei 
Published: October 2023

Background::
Coronavirus disease 2019 (COVID-19) not only causes a range of respiratory symptoms
but also has a great impact on individual mental health. With the global pandemic of SARS-CoV-2, the
incidence of COVID-19 comorbid with depression has increased significantly. Curcumin, a natural polyphenol
compound, has been shown to have antidepressant and anti-coronavirus activities.

Methods::
This study aimed to explore the molecular targets and underlying biological mechanisms of curcumin
in the treatment of COVID-19 with depression through an integrative pharmacology strategy, including
target prediction, network analysis, PPI analysis, GO and KEGG enrichment analyses, and molecular docking.

Results::
After a comprehensive search and thorough analysis, 8 core targets (ALB, AKT1, CASP3, STAT3,
EGFR, PTGS2, FOS, and SERPINE1) were identified. GO and KEGG enrichment analysis results revealed
that the pathways related to viral infection, immune regulation, neuronal reorganization, apoptosis, and secretion
of inflammatory cytokines were involved in the pathological process. Furthermore, molecular docking
showed that curcumin could spontaneously bind to the SARS-CoV-2-related receptor proteins and the core
targets with a strong binding force.

Conclusion::
The potential pharmacological mechanisms of curcumin in COVID-19 comorbid depression
were evaluated. Curcumin can be used as a therapeutic agent for COVID-19 comorbid depression. One of the
potential mechanisms may be to reduce the inflammatory response and suppress the cytokine storm by regulating
the JAK-STAT signaling pathway and MAPK signaling pathway. These findings may help to overcome
the impact of the COVID-19 pandemic on psychological health.
Novel Aptamers for the Reactivation of Latent HIV
Authors: Serumula William , Nkambule Bongani , Parboosing Raveen 
Published: October 2023

Introduction:
A “Shock and Kill” strategy has been proposed to eradicate the HIV latent
viral reservoir. Effective Latency Reversal Agents (LRA) are a key requirement for this strategy.
The search for LRAs with a novel mechanism of action is ongoing. This is the first study to
propose aptamers for the reactivation of HIV.

Objective:
The purpose of this study was to identify an aptamer that potentially reactivates HIV
via the NF-κβ pathway, specifically by binding to IkB and releasing NF-κβ.

Methods:
Aptamer selection was performed at Aptus Biotech (www.aptusbiotech.es), using ikB
human recombinant protein with His tag bound to Ni-NTA agarose resin using the SELEX procedure.
Activation of NF-κβ was measured by SEAP Assay. HIV reactivation was measured in JLat
cells using a BD FACS-Canto™ II flow cytometer. All flow cytometry data were analyzed using
Kaluza analyzing software.

Results:
Clones that had equivalent or greater activation than the positive control in the SEAP assay
were regarded as potential reactivators of the NF-κβ pathway and were sequenced. The three
ikb clones namely R6-1F, R6-2F, and R6-3F were found to potentially activate the NF-κβ pathway.
Toxicity was determined by exposing lymphocytes to serial dilutions of the aptamers; the
highest concentration of the aptamers that did not decrease viability by > 20% was used for the reactivation
experiments. The three novel aptamers R6-1F, R6-2F, and R6-3F resulted in 4,07%,
6,72% and 3,42% HIV reactivation, respectively, while the untreated control showed minimal
(<0.18%) fluorescence detection.

Conclusion:
This study demonstrated the reactivation of latent HIV by aptamers that act via the
NF-κβ pathway. Although the effect was modest and unlikely to be of clinical benefit, future
studies are warranted to explore ways of enhancing reactivation.
Synthesis and Anticancer Activity of Oral Pt(IV) Prodrugs Containing α-furancarboxylate as an Axial Ligand
Authors: Gao Anli , Zhou Peng , Yu Juan , Luo Min , Jiang Jing , Zhang Ling , Liu Weiping , Qing Chen 
Published: October 2023

Background:
The clinical applications of platinum-based anticancer drugs are largely
compromised by side effects and drug resistance. Therefore, novel platinum-based anticancer drugs
with improved injected or oral therapeutic index and low resistance need to be developed.

Objective:
This study aimed at the synthesis and anticancer activity testing of Pt(IV) prodrugs containing
α-furancarboxylate as an axial ligand. This would pave the way for obtaining novel Pt(IV)
prodrugs with better anticancer activity by comparing the anticancer activity with their parent platinum(
II) complexes.

Methods:
In this study, synthesis, in vitro cytoxicity assay, and in vivo anticancer activity evaluation of
three Pt(IV) complexes, cis,trans,cis-[Pt(NH3)2(OH)(α-furancarboxylato)Cl2] (FPt-1), cis,trans,cis-
[Pt(NH3)2(OH)(α-furancarboxylato)(1,1'-cylobutanedicarboxylato)] (FPt-2), and cis,trans,cis-
[Pt(1R,2R-diaminocyclohexane)(OH)(α-furancarboxylato)(C2O4)] (FPt-3), were carried out.

Results:
Three Pt(IV) complexes exhibited considerable cytoxicity against the tested human cancer
cells (MCF-7, A549 and HCT116), which was found to be slightly lower than the corresponding
Pt(II) drugs. However, FPt-1 and FPt-3 displayed comparable antitumor efficacy to cisplatin and
oxaliplatin in the murine S180 sarcoma model after intraperitoneal administration. More importantly,
the intragastric administration test indicated the antitumor efficacy of FPt-3 to be much greater than
oxaliplatin.

Conclusion:
FPt-3 has shown excellent oral antitumor activity and it could be administrated in an
oral dosage form.
When Pulmonary Arterial Hypertension may be Associated with Portal
Hypertension: A Case Report of Two Different Hepatic Disorders in One
Patient with Pulmonary Hypertension
Authors: Radchenko Ganna D. , Sirenko Yuriy M. 
Published: October 2023

Background::
pulmonary arterial hypertension (PAH) is a rare complication of hepatic
diseases with portal hypertension that, however, has a significant influence on prognosis. We
present a mini-review of how to diagnose and treat it based on a clinical case.

Case Presentation:
in early childhood, a patient had portal hypertension associated
with cavernous transformation of the portal vein. It was successfully treated by reno-splenic surgery.
At the age of 20 years, this patient experienced increased dyspnea at minimal physical activity
after the hepatic biopsy due to a hepatocellular adenoma. The examination in the specialized
unit showed PAH, which was evaluated as associated with portal hypertension (PAH-PoH).
The specific two-drug combination therapy was started with prominent improvement in patient’s
state. Successful surgical tumor treatment was provided some months later. The practical and
clinical approaches to the diagnosis and treatment of PAH-PoH are discussed. It was emphasized
that not all patients with portal hypertension have pulmonary hypertension, which needs to
be treated. A lot of evidence gaps exist in management of these patients.

Conclusion::
all patients, even with past history of portal hypertension, should be monitored
closely and screened for PAH earlier, for better results of treatment.
Intrahepatic Pseudoaneurysm Developing at the Confluence of Bilateral Biliary
Drains Six Months Following a Procedure- A Case Report
Authors: Qtaish Izzeddin , Ayasrah Mohammad , Qtaish Noat R. 
Published: October 2023

Background::
Hepatic artery aneurysms (HAAs) exhibit a notable risk of rupture, with both true aneurysms and pseudoaneurysms being exceptionally
uncommon (incidence rate approximately 2 per 100,000). Symptoms include epigastric and right upper quadrant pain, jaundice, and potentially
life-threatening bleeding upon rupture. This bleeding can extend into the portal vein, hepatic vein, biliary system, or abdominal cavity. While
existing literature lacks reports of delayed post-therapeutic complications, this case report discusses a 27-year-old female who developed a
pseudoaneurysm six months after bilateral biliary drain insertion, underscoring the potential for delayed complications.

Case Presentation::
The patient underwent gastric endoscopy revealing an extensive lower esophageal varix protruding into the proximal stomach, subsequently treated
with banding. The stomach and duodenum were found to contain fresh blood and blood clots indicating acute bleeding.

:
An urgent CT scan identified active bleeding (extravasation) at segment V and blood accumulation at the confluence of the right and left biliary
drains. This was followed by celiac and selective hepatic digital subtraction angiography (DSA).

Conclusion::
This case study outlines the occurrence of a hepatic artery pseudoaneurysm six months after percutaneous insertion of bilateral biliary drains. The
pseudoaneurysm manifested precisely at the confluence of the right and left drains. The reported delayed complication may have resulted from
friction between the drains at the confluence or erosion of the adjacent artery by the drains. The insights gained from this case shed light on the
causes and preventive measures for potential delayed complications associated with percutaneous biliary drain placement.
Antidiabetic Activity and HPTLC Analysis of a Classical Indian Herbomineral
Formulation – Vidangadilouham
Authors: Debnath Monojit , Roy Moulisha Biswas , Sarkar Debdip , Ghosh Chowdhury Payel , Goswami Ayan , Bala Asis , Haldar Pallab Kanti 
Published: October 2023

Background::
Vidangalilouham (VDL) is an Ayurveda classical Indian herbomineral preparation prescribed for its effective antidiabetic activity. Among the
official formulations of Ayurveda, five different compositions are available by the same name. The particular composition used in this study is
only marketed but has yet to be evaluated.

Aim and Objective::
The study aimed to assess and validate the antihyperglycemic activity of VDL in high-fat and low-dose streptozotocin (STZ)-induced diabetic
animal models.

Methods::
High-fat diet-induced animal model of hyperglycemia was generated by a single intraperitoneal injection of STZ (35 mg/kg body weight) into the
rats fed with high fat for the previous 30 days. Seven days after STZ induction, the hyperglycemic rats were treated with VDL orally at 100 and
200 mg/kg body weight daily for 28 days. The fasting blood glucose levels (FBG) were measured every 7th day during the 28 days of treatment.
The glycosylated hemoglobin levels, liver and serum biochemical parameters, and histopathological findings were estimated and compared.

Results and Discussion::
VDL-treated animals significantly exhibited reduced FBG levels compared to the diabetic control group. The lipid peroxidation and antioxidant
enzyme levels, such as CAT, GSH, and SOD, in the VDL-treated groups, were restored toward normal levels compared to diabetic control groups,
and the values were comparable with the standard group (metformin). The critical diabetic marker, i.e., glycosylated hemoglobin levels were
significantly (*p<0.05) decreased when treated with VDL.

Conclusion::
Improvement in the FBG and the restoration of all other biomarkers observed in this study indicate VDL to have excellent antidiabetic activity with
meager side effects, and thus, the findings provide a scientific rationale for its use as an antidiabetic agent.
Unlocking the Microbial Potential of Intercalated Calcium-aluminum
Layered Double Hydroxide-palmitic Acid
Authors: Johari Nur Adlina , Sheikh Mohd Ghazali Sheikh Ahmad Izaddin , Ismail Nurul Huda , Ariffin Suhaidi , Poddar Sandeep , Biswas Hari Shankar 
Published: October 2023

Introduction:
An efficient and coherent drug delivery system is imperative in detouring
a repetitive administration of high doses of the drug to achieve an effective therapeutic effect. This
study, therefore, aims to synthesize the nanocomposite (CAPA) utilizing the layered double hydroxide
as a drug carrier that can safeguard the medicine and improve its bioavailability while minimizing
the adverse impact on the biological process.

Method:
The Calcium-aluminum Layered Double Hydroxide (CAL) was synthesized via the coprecipitation
method followed by integrating palmitic acid (PA) drug into that host employing a
similar approach. The successful intercalation was assessed utilizing X-ray diffraction (PXRD)
analysis and Fourier transform infrared spectroscopy (FTIR). The characterization of the material
was evaluated by using a thermogravimetric-derivative thermogravimetric analysis (TGA-DTG)
and accelerated surface area and porosity (ASAP) analyzer.

Result:
The increment of basal spacing of CAPA (15.21Å) synthesized in this study implies the retainment
of PA in the interlayer space of CAL. The FTIR spectra of CAPA, with the elimination of
the nitrate ion peak at 1359.87 cm-1 and the appearance of carboxylate ion at 1643.17 cm-1, hint at
the existence of PA in the host layer. The surface area of CAPA exhibited a value of 19.8 m2g-1,
bigger than that of hosts, while its pore size is within the micropores range.

Conclusion:
The TGA analysis revealed that the thermal stability of PA was improved following
the intercalation process due to the decomposition of the PA core that occurs at 260°C. The antimicrobial
activity proposes that the synthesized CAPA can retain the drug's activity against S. aureus,
emphasizing the ability of CAL as a potential drug delivery vehicle for PA.
A Structure-based Data Set of Protein-peptide Affinities and its
Nonredundant Benchmark: Potential Applications in Computational
Peptidology
Authors: Wang Shaozhou , Ye Haiyang , Shang Shuyong , Li Zilong , Peng Yue , Zhou Peng 
Published: October 2023

Background:
Peptides play crucial roles in diverse cellular functions and participate
in many biological processes by interacting with a variety of proteins, which have
also been exploited as a promising class of therapeutic agents to target druggable proteins
over the past decades. Understanding the intrinsic association between the structure
and affinity of protein-peptide interactions (PpIs) should be considerably valuable for the
computational peptidology area, such as guiding protein-peptide docking calculations, developing
protein-peptide affinity scoring functions, and designing peptide ligands for specific
protein receptors.

Objective:
We attempted to create a data source for relating PpI structure to affinity.

Methods:
By exhaustively surveying the whole protein data bank (PDB) database as well
as the ontologically enriched literature information, we manually curated a structure-
based data set of protein-peptide affinities, PpI[S/A]DS, which assembled over 350
PpI complex samples with both the experimentally measured structure and affinity data.
The data set was further reduced to a nonredundant benchmark consisting of 102 culled
samples, PpI[S/A]BM, which only selected those of structurally reliable, functionally diverse
and evolutionarily nonhomologous.

Results:
The collected structures were resolved at a high-resolution level with either Xray
crystallography or solution NMR, while the deposited affinities were characterized
by dissociation constant, i.e. Kd value, which is a direct biophysical measure of the intermolecular
interaction strength between protein and peptide, ranging from subnanomolar
to millimolar levels. The PpI samples in the set/benchmark were arbitrarily classified into
α-helix, partial α-helix, β-sheet formed through binding, β-strand formed through selffolding,
mixed, and other irregular ones, totally resulting in six classes according to the
secondary structure of their peptide ligands. In addition, we also categorized these PpIs
in terms of their biological function and binding behavior.

Conclusion:
The PpI[S/A]DS set and PpI[S/A]BM benchmark can be considered a valuable
data source in the computational peptidology community, aiming to relate the affinity to
structure for PpIs.
Rasionalitas Terapi Antibotik Empiris Pada Pasien Geriatri Dengan Pneumonia Di RS Mardi Rahayu
Authors: Ikawati, Apt. Zullies 
Published: October 2023

Pneumonia adalah penyakit infeksi akut yang mengenai jaringan (paru-paru) tepatnya di alveoli. Insiden pneumonia meningkat seiring bertambahnya usia karena perubahan fisiologis dan status imunologi yang terkait dengan penuaan dan adanya komorbiditas pada usia lanjut. Pneumonia merupakan penyakit infeksi terbesar di RS Mardi Rahayu pada tahun 2020 sampai dengan 2022. Pemilihan antibiotik empiris yang tidak tepat menyebabkan resistensi antibiotik dan penggunaannya yang terlalu lama dapat meningkatkan lama perawatan sehingga biaya perawatan meningkat. Analisa rasionalitas penggunaan antibiotik empiris yang spesifik pada pasien geriatri dengan pneumonia di RS Mardi Rahayu belum pernah dilakukan. Penelitian ini menggunakan rancangan deskriptif dan analitik observasional dengan metode cross-sectional. Analisa terhadap rasionalitas terapi antibiotik empiris menggunakan metode Gyssens. Hasil penelitian menunjukkan jumlah pasien geriatri yang didiagnosa pneumonia yang dirawat inap di RS Mardi Rahayu periode Januari - Desember 2022 yang memenuhi kriteria inklusi adalah 139 pasien dengan jumlah 158 regimen antibiotik. Hasil analisa rasionalitas menunjukkan bahwa sebanyak 95 regimen antibiotik (60,13%) termasuk kategori 0 (nol) yang artinya penggunaan antibiotik rasional. Ketidakrasionalan penggunaan antibiotik terjadi pada 63 regimen pada kategori IV-A sebanyak 39 kasus (24,68%), IV-B sebanyak 9 kasus (5,70%), IV-C sebanyak 2 kasus (1,27%), III-A sebanyak 9 kasus (5,70%), III-B sebanyak 20 kasus (12,66%) dan II-A sebanyak 2 kasus (1,27%).
PELATIHAN ANALISIS DATA PENELITIAN BAGI GURU SDN 2 SEGALAMIDER BANDAR LAMPUNGDENGAN MENGGUNAKAN APLIKASI SPSS
Authors: Nusyirwan Nusyirwan , Setiawan Eri , Ruby Tiryono , Saidi Subian 
Published: October 2023

Guru sebagai sumberdaya manusia dalam dunia pendidikan dituntut untuk melakukan pengembangan diri. Guru tidak saja dituntut menguasai materi ajaran atau mampu menyajikan secara tepat, tetapi juga dituntut mampu melakukan pengembangan pengajaran di dalam kelas melalui penelitian. Jenis penelitian yang dapat dilakukan oleh guru cukup banyak di antaranya adalah Penelitian Tindakan Kelas (PTK). Sebagian besar guru, baik guru SD/MI, SMP/MTs, dan SMA/MA menyatakan bahwa pada analisis data inilah bagian yang tersulit dalam PTK. Hal ini tidaklah 100% benar, karena sebenarnya pada bagian inilah hal yang sangat mengasyikkan. Orang akan bisa karena terbiasa, orang akan tidak bisa karena belum terbiasa. Kata-kata itu nampaknya benar, artinya guru merasa tidak bisa melakukan analisis data dalam PTK karena memang belum terbiasa. Untuk membiasakan guru-guru dalam menganalisis data penelitiannya diperlukan aplikasi program statistika. Salah satu aplikasi program statistika adalah SPSS. Dengan analisis data menggunakan aplikasi SPSS sangat membantu guru untuk meningkatkan kualitas penelitiannya. Oleh karena itu, kegiatan pengabdian kepada masyarakat ini dirasakan perlu untuk membantu guru-guru dalam teknik analisis data dengan SPSS untuk meningkatkan kemampuan menelitinya. Secara umum penyelenggaraan kegiatan pelatihan Analisis Data Menggunakan Aplikasi SPSS di SDN 2 Segalamider Bandar Lampung memperoleh sambutan yang sangat antusias. Oleh karena itu hasil kegiatan pelatihan ini memberi hasil yang “SANGAT BAIK” . Ini ditandai oleh peningkatan hasil yang memuaskan dilihat dari hasil ujian akhir (pstest) peserta pelatihan yang mengalami peningkatan 85 % dari kondisi awal.
Analysis of Sample Science Questions for Secondary School Exam in terms of Revised Bloom Taxonomy
Authors: ZERMAN KEPCEOĞLU Gülşah , PEKTAŞ Murat 
Published: October 2023

Purpose: In this research, it is aimed to examine the sample questions for the exam in high school transition system published between 2018-2022 according to the revised bloom taxonomy.
Design/Methodology/Approach: The document analysis model was used and The data source of this research is the science questions in the sample questions for the central examination for secondary education institutions to admit students by examination published on the website of the General Directorate of Measurement, Evaluation and Examination by the Ministry of National Education since October 2018. Between October 2018 and December 2022, a total of 355 science questions were published and in this study all of these questions examined according to the revised bloom taxonomy.
Findings: Most of the sample questions examined are within the scope of conceptual questions. 74% of the questions published in 2018, 75% of the questions in 2019, 66% of the questions in 2020 and 2021, and 70% of the questions in 2022 are at the level of conceptual knowledge.
Highlights: Almost all of the questions are in the conceptual knowledge class and are preferred from low-level questions, and high-level questions are the last ones. Another important finding is that all of the high-level questions (58 questions) are preferred from the category “Analyze”, but not at all from the categories “Evaluate” and “Create”.
Pembuatan Standar Operasional Prosedur (SOP) Praktikum Untuk Meningkatkan Efesiensi Pelasanaan Pratikum Fisika SMA
Authors: Handayani Sri , Suseno Nyoto , Alarifin Dedy Hidayatullah 
Published: October 2023

ABSTRACTThe problem in this research is that the management of the physics laboratory is still not optimal. Therefore, it is necessary to have a managementsystem or management of laboratory implementation in a good physics laboratoryby creating standard operating procedures (SOP) for practicum implementation. The aim to be achieved in this study is to produce an SOP for practicum implementation in the utilization of physics laboratories in senior high school and to determine the efficiency of the implementation of senior high school physics practicum after using the developed SOP. The development model used in this research is the ADDIE development model, which is an extension of Analysis, Design, Development, Implementation, and Evaluation. The product feasibility level is seen from the results of validation from laboratory management experts, the results of the feasibility percentage of practicum SOP are 83.33%, and the percentage of students' responses obtained from the product trial results is91.38%. Thus, it can be concluded that the practicum SOP developed is suitable for use in the process of practical activities.
Keywords: standard operational procedure, efficiency, and practicum.
Revitalizing The Beas Perelek Tradition: Actualization of Patriotism During The Covid-19 Pandemic
Authors: Mubarok Muhamad Sofi , Ahmad Tarmidzi Ayi Yusri 
Published: October 2023

Sebagai negara multikultural Indonesia mengenal tradisi ‘Gotong Royong’. Salah Satu bentuk tradisi gotong royong tersebut adalah Beas Perelek, yaitu tradisi yang lahir dan berkembang sebagai wujud kepedulian sosial dan usaha untuk menjaga stabilitas sebuah lingkungan melalui kesejahteraan ekonomi masyarakatnya. Tradisi ini berupa pendistribusian beras kepada masyarakat pra-sejahtera yang didapat dari urunan setiap masyarakat. Beas Perelek diklaim mampu meningkatkan rasa solidaritas, menumbuhkan sikap patriotisme, dan mengatasi masalah kesejahteraan ekonomi masyarakat yang dewasa ini mengalami penurunan yang siginifikan akibat pandemi Covid-19. Melalui kajian tafsir tematik, nilai tradisi Beas Perelek ini memiliki kedudukan yang agung dalam Islam. Beas Perelek merupakan aktualisasi nyata dari konsep At-Ta`awun (tolong menolong) dalam Islam. Metodologi yang digunakan dalam paper ini adakah pendekatan kualitatif dengan analisa deskriptif. Paper ini bertujuan menganalisis secara kritis-komprehensif terhadap tradisi Beas Perelek yang dewasa ini dapat menjadi solusi sederhana dalam mengatasi krisis kesejahteraan ekonomi masyarakat Indonesia.