bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accessible Ni‐Fe‐Oxalate Framework for Electrochemical Urea Oxidation with Radically Enhanced Kinetics
Authors: Kim Jiseon , Kim Min‐Cheol , Han Sang Soo , Cho Kangwoo 
Published: January 2024

AbstractUrea oxidation reaction (UOR) has been utilized to substitute the oxygen evolution reaction (OER), to escalate the energy conversion efficiency in electrochemical hydrogen generation processes with denitrification of widespread urea in wastewater. This study reports breakthroughs in Ni‐based UOR electrocatalysts, particularly with NiFe oxalate (O‐NFF), derived from Ni3Fe alloy foam with prismatic nanostructures and elevated surface area. The O‐NFF achieves cutting‐edge performances, representing 500 mA cm−2 of current density at 1.47 V RHE and exceptionally low Tafel slope of 12.1 mV dec−1 (in 1 m KOH with 0.33 m urea). X‐ray photoelectron/absorption spectroscopy (XPS/XAS) coupled with density functional theory calculations unveil that oxalate ligands induce charge deficient Ni center, promoting stable urea‐O adsorption. Furthermore, Fe dopants enhance oxalate‐O charge density and H‐bond strength, facilitating C‐N cleavage for N2 and NO2− formation. The extraordinary UOR kinetics by the tandem effects of oxalate and Fe prevent Ni over‐oxidation, corroborated by operando XAS, minimizing OER interference. It agrees with an adaptive reconstruction to Fe‐doped β‐NiOOH on top surface in extended urea electrolysis with marginal loss in UOR kinetics. This findings shed light to bimetal‐organic‐framework as (pre)catalysts to improve industrial electrolytic H2 production.
Controllable Assembly of Cu2+ and Chlorin E6 for H2S‐Activatable Recognition of Bacterial Infection and Enhanced Antibacterial Therapy
Authors: Wang Henggang , Cheng Ke , Sun Shan , Wang Peng , Zhou Yonghua , Sun Haoyi , Wang Xinxin , Shen Hongzhe , Li Si , Lin Hengwei 
Published: January 2024

AbstractAntibacterial photodynamic therapy (APDT) has emerged as one of the intriguing strategies to combat bacterial resistance. However, the antibacterial efficacy of APDT is found to be severely impacted by the hydrogen sulfide (H2S)‐overproduced bacterial infection microenvironment. Herein, a multifunctional APDT platform is developed by assembling Cu2+ and chlorin e6 (Ce6), which exhibits unique H2S‐activatable fluorescence (FL) and antibacterial features. Noteworthily, the assembly conditions are crucial for achievement of Cu‐Ce6 nanoassemblies (NAs) with the on‐demand responsive properties. The quenched FL and photosensitization of Cu‐Ce6 NAs can be selectively activated by the overexpressed H2S in infected area, enabling specific recognition of bacterial infection and localized antibacterial therapy with minimized side effects. Significantly, amplified oxidative stress is achieved owning to the effective consumption of H2S by Cu2+ in the NAs, leading to an enhanced APDT. The antibacterial mechanisms including broad‐spectrum APDT activity of released Ce6, inherent sterilization effects of produced copper polysulfides and the accompanying disturbance of bacterial sulphide metabolism are further identified. This study may pave a new avenue for the rational design of intelligent APDT platform using minimalist biological building units and thus facilitating the clinical translation of nano‐antibacterial agents.
Early Diagnosis and Prognosis Prediction of Pancreatic Cancer Using Engineered Hybrid Core‐Shells in Laser Desorption/Ionization Mass Spectrometry
Authors: Zhang Juxiang , Teng Fei , Hu Beiyuan , Liu Wanshan , Huang Yida , Wu Jiao , Wang Yuning , Su Haiyang , Yang Shouzhi , Zhang Lumin , Guo Lingchuan , Lei Zhe , Yan Meng , Xu Xiaoyu , Wang Ruimin , Bao Qingui , Dong Qiongzhu , Long Jiang , Qian Kun 
Published: January 2024

AbstractEffective detection of bio‐molecules relies on the precise design and preparation of materials, particularly in laser desorption/ionization mass spectrometry (LDI‐MS). Despite significant advancements in substrate materials, the performance of single‐structured substrates remains suboptimal for LDI‐MS analysis of complex systems. Herein, designer Au@SiO2@ZrO2 core‐shell substrates are developed for LDI‐MS‐based early diagnosis and prognosis of pancreatic cancer (PC). Through controlling Au core size and ZrO2 shell crystallization, signal amplification of metabolites up to 3 orders is not only achieved, but also the synergistic mechanism of the LDI process is revealed. The optimized Au@SiO2@ZrO2 enables a direct record of serum metabolic fingerprints (SMFs) by LDI‐MS. Subsequently, SMFs are employed to distinguish early PC (stage I/II) from controls, with an accuracy of 92%. Moreover, a prognostic prediction scoring system is established with enhanced efficacy in predicting PC survival compared to CA19‐9 (p < 0.05). This work contributes to material‐based cancer diagnosis and prognosis.
Shifting Access from Pyrimidine‐Spirofused to Fused Benzoheterocycles by Modifying the Activated Group Position
Authors: Yang Dezhi , Wang Yan , Zhang Chun , Luo Yun , Zhu Xuecheng , Zhao Si , Fu Wenting , Cheng Bin , Zhai Hongbin , Wang Taimin 
Published: January 2024

AbstractThe synthesis of pyrimidine‐spirofused indolines from 1,3,5‐triazinanes with 2‐sulfonyliminoindolines has been achieved under catalyst‐ and additive‐free conditions, in which five atoms of 1,3,5‐triazinanes were introduced to the spiro‐annulation products via a (5+1) pathway. Subsequently, this strategy was extended to 3‐aminoindoles and 3‐aminobenzothiophenes, but a different reaction pathway (3+3) was observed. In these cases, three‐atoms of 1,3,5‐triazinanes were incorporated into pyrimidine‐fused indoles/benzothiophenes. These two transformations demonstrate the potential and versatility of 1,3,5‐triazinanes to construct nitrogen‐heterocycles.
RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles
Authors: Wang Liying , Liu Chao , Li Li , Wei Huafang , Wei Wei , Zhou Qiuxing , Chen Yinghong , Meng Tie‐Gang , Jiao Renjie , Wang Zhen‐Bo , Sun Qing‐Yuan , Li Wei 
Published: January 2024

AbstractPreviously a ring finger protein 20 (RNF20) is found to be essential for meiotic recombination and mediates H2B ubiquitination during spermatogenesis. However, its role in meiotic division is still unknown. Here, it is shown that RNF20 is localized at both centromeres and spindle poles, and it is required for oocyte acentrosomal spindle organization and female fertility. RNF20‐depleted oocytes exhibit severely abnormal spindle and chromosome misalignment caused by defective bipolar organization. Notably, it is found that the function of RNF20 in spindle assembly is not dependent on its E3 ligase activity. Instead, RNF20 regulates spindle assembly by recruiting tropomyosin3 (TPM3) to both centromeres and spindle poles with its coiled‐coil motif. The RNF20‐TPM3 interaction is essential for acentrosomal meiotic spindle assembly. Together, the studies uncover a novel function for RNF20 in mediating TPM3 recruitment to both centromeres and spindle poles during oocyte spindle assembly.
A Natural Small Molecule Mitigates Kidney Fibrosis by Targeting Cdc42‐mediated GSK‐3β/β‐catenin Signaling
Authors: Hu Xinrong , Gan Lu , Tang Ziwen , Lin Ruoni , Liang Zhou , Li Feng , Zhu Changjian , Han Xu , Zheng Ruilin , Shen Jiani , Yu Jing , Luo Ning , Peng Wenxing , Tan Jiaqing , Li Xiaoyan , Fan Jinjin , Wen Qiong , Wang Xin , Li Jianbo , Zheng Xunhua , Liu Qinghua , Guo Jianping , Shi Guo‐Ping , Mao Haiping , Chen Wei , Yin Sheng , Zhou Yi 
Published: January 2024

AbstractKidney fibrosis is a common fate of chronic kidney diseases (CKDs), eventually leading to renal dysfunction. Yet, no effective treatment for this pathological process has been achieved. During the bioassay‐guided chemical investigation of the medicinal plant Wikstroemia chamaedaphne, a daphne diterpenoid, daphnepedunin A (DA), is characterized as a promising anti‐renal fibrotic lead. DA shows significant anti‐kidney fibrosis effects in cultured renal fibroblasts and unilateral ureteral obstructed mice, being more potent than the clinical trial drug pirfenidone. Leveraging the thermal proteome profiling strategy, cell division cycle 42 (Cdc42) is identified as the direct target of DA. Mechanistically, DA targets to reduce Cdc42 activity and down‐regulates its downstream phospho‐protein kinase Cζ(p‐PKCζ)/phospho‐glycogen synthase kinase‐3β (p‐GSK‐3β), thereby promoting β‐catenin Ser33/37/Thr41 phosphorylation and ubiquitin‐dependent proteolysis to block classical pro‐fibrotic β‐catenin signaling. These findings suggest that Cdc42 is a promising therapeutic target for kidney fibrosis, and highlight DA as a potent Cdc42 inhibitor for combating CKDs.
Chemotherapy‐Enabled Colorectal Cancer Immunotherapy of Self‐Delivery Nano‐PROTACs by Inhibiting Tumor Glycolysis and Avoiding Adaptive Immune Resistance
Authors: Zhao Lin‐Ping , Zheng Rong‐Rong , Rao Xiao‐Na , Huang Chu‐Yu , Zhou Hang‐Yu , Yu Xi‐Yong , Jiang Xue‐Yan , Li Shi‐Ying 
Published: January 2024

AbstractThe chemo‐regulation abilities of chemotherapeutic medications are appealing to address the low immunogenicity, immunosuppressive lactate microenvironment, and adaptive immune resistance of colorectal cancer. In this work, the proteolysis targeting chimera (PROTAC) of BRD4 (dBET57) is found to downregulate colorectal cancer glycolysis through the transcription inhibition of c‐Myc, which also inhibits the expression of programmed death ligand 1 (PD‐L1) to reverse immune evasion and avoid adaptive immune resistance. Based on this, self‐delivery nano‐PROTACs (designated as DdLD NPs) are further fabricated by the self‐assembly of doxorubicin (DOX) and dBET57 with the assistance of DSPE‐PEG2000. DdLD NPs can improve the stability, intracellular delivery, and tumor targeting accumulation of DOX and dBET57. Meanwhile, the chemotherapeutic effect of DdLD NPs can efficiently destroy colorectal cancer cells to trigger a robust immunogenic cell death (ICD). More importantly, the chemo‐regulation effects of DdLD NPs can inhibit colorectal cancer glycolysis to reduce the lactate production, and downregulate the PD‐L1 expression through BRD4 degradation. Taking advantages of the chemotherapy and chemo‐regulation ability, DdLD NPs systemically activated the antitumor immunity to suppress the primary and metastatic colorectal cancer progression without inducing any systemic side effects. Such self‐delivery nano‐PROTACs may provide a new insight for chemotherapy‐enabled tumor immunotherapy.
Functional copolymer coatings on electrospun fibers via photo‐initiated chemical vapor deposition
Authors: Güney Eda , Karimzadeh Khoei Jalal , Sengul Bengu Sueda , Can Faruk , Ozaydin Ince Gozde 
Published: January 2024

AbstractWith the increasing use of implantable patches in biomedical applications, the significance of surface modification techniques in improving biocompatibility, enhancing adhesion, and regulating drug release has grown. A significant challenge that these methods must address is ensuring that the process does not harm the delicate fibers or therapeutic agents they contain. Here, we report surface functionalization of implantable, curcumin loaded Polycaprolactone (PCL) patches with pH‐responsive poly(2‐hydroxyethyl methacrylate‐co‐4‐vinylpyridine), p(HEMA‐co‐4VP) polymer thin film. The polymer was coated on the patch surface via photo‐initiated chemical vapor deposition (piCVD) where the polymerization was initiated by the UV degradation of the initiator tert‐butyl peroxide (TBPO) and the monomer HEMA. Additionally, the piCVD method was utilized to crosslink the HEMA without the use of additional crosslinkers. The pH‐responsiveness of the coating was achieved by incorporating 4VP into the copolymer structure. The effect of the coating was demonstrated through degradation and drug release studies. The presence of the polymer coating decelerated the fiber degradation and the pH‐dependent swelling of the coating allowed for the control of drug release rates from the patches. The innovative use of piCVD as a coating method provides a platform for advancing tailored surface modifications in various biomedical applications.
Application of augmented reality models of canine skull in veterinary anatomical education
Authors: Jiang Nan , Jiang Zhongling , Huang Yufeng , Sun Mingju , Sun Xuejing , Huan Yanjun , Li Fangzheng 
Published: January 2024

AbstractVeterinary anatomy plays a crucial role in the curriculum for veterinary medicine and surgery. The integration of modern information technology in veterinary education can greatly benefit from innovative tools such as augmented reality (AR) applications. The aim of this study was to develop an accurate and interactive three‐dimensional (3D) digital model of an animal skull using AR technology, aiming to enhance the learning of skull anatomy in veterinary anatomy education. In this study, a canine skull specimen was isolated, and the skull bones were scanned using a structured light scanner to create a 3D digital model of the canine skull, which was found to be indistinguishable from the original specimen by measurement of skull proportions. Furthermore, the interactive AR model of the canine skull, displayed using Unity3D, was subjected to testing and evaluation by 60 first‐year veterinary medical students attending the gross anatomy of the animal. The students were divided into two groups: the traditional group and AR group. Both groups completed an objective test and a questionnaire. The evaluation of learning effectiveness in the test revealed no significant difference between the traditional group (which learned using textbooks and a canine skull specimen) and AR group (which learned using AR tools). However, in the questionnaire, students displayed high enthusiasm and interest in using the AR tool. Therefore, the application of AR tools can improve students' motivation for learning and enhance the comprehension of anatomical structures in three dimensions. Furthermore, this study exemplifies the use of AR as an auxiliary tool for teaching and learning in veterinary anatomy education.
Overcoming strategies for supply chain digitization barriers: Implications for sustainable development goals
Authors: Dadsena Krishna Kumar , Pant Pushpesh , Paul Sanjoy Kumar , Pratap Saurabh 
Published: January 2024

AbstractThis paper examines barriers to supply chain digitization (SCD) and offers mitigation strategies, focusing on sustainable development goals (SDGs), technological adoption, and financial constraints. A hybrid research approach, combining qualitative and quantitative methods, has been used to analyze significant SCD barriers and strategies under budget constraints. According to the findings, “lack of proper research and development facility” is the most influential barrier to SCD. The overcoming strategies are then ranked by how likely they are to be implemented in response to barriers. From a sustainability perspective, “improving privacy and security” is identified as the most important strategy. Further, a mathematical model is developed to facilitate investment‐related decision‐making by determining the most suitable overcoming strategies under budget constraints. The proposed mathematical model is evaluated across a range of investments and for a set of overcoming strategies within a given scenario. The findings demonstrate that optimal investments of $1.78 million can cover the top two overcoming strategies for implementation. Furthermore, the results indicate that a minimum investment of $1.9 million is required to adopt all 10 overcoming strategies.
EVERSYS: Efficient exam versioning tool for linear circuits and other problem‐based subjects
Authors: Vicente María Asunción , Fernández Peris César , Martínez Miguel O. 
Published: January 2024

AbstractExam versioning is a widely used approach to prevent cheating during exams, but it can increase the workload for lecturers in terms of exam preparation and correction. Automated exam versioning can help reduce this workload and minimize errors in correction. This article presents the EVERSYS platform, which is focused on linear circuits but can be applied to other engineering fields. An exam problem is described as a circuit schema, a set of component values, and a set of questions to be answered. The EVERSYS platform allows lecturers to easily create exam versions with high variability, including different schemas, component values, and question sets. The platform is based on the MATLAB/Octave programming language and HTML document descriptions. Creating a multiversion exam is straightforward, involving the adaptation of a set of predefined MATLAB scripts and modification of the HTML format if necessary. A set of example exams is provided as well as the complete platform source code. The platform was tested during a 2021–2022 compulsory course on linear circuits at a Spanish University. The results indicated that the platform significantly reduced the time required for exam preparation and correction, and there was no statistically significant difference in student performance across different exam versions. Students reported high levels of satisfaction with the platform, and almost all agreed that it effectively prevents cheating during exams.
Gut microbiota, microbiota‐derived metabolites, and graft‐versus‐host disease
Authors: Yue XiaoYan , Zhou Hongyu , Wang ShuFen , Chen Xu , Xiao HaoWen 
Published: January 2024

AbstractAllogeneic hematopoietic stem cell transplantation is one of the most effective treatment strategies for leukemia, lymphoma, and other hematologic malignancies. However, graft‐versus‐host disease (GVHD) can significantly reduce the survival rate and quality of life of patients after transplantation, and is therefore the greatest obstacle to transplantation. The recent development of new technologies, including high‐throughput sequencing, metabolomics, and others, has facilitated great progress in understanding the complex interactions between gut microbiota, microbiota‐derived metabolites, and the host. Of these interactions, the relationship between gut microbiota, microbial‐associated metabolites, and GVHD has been most intensively researched. Studies have shown that GVHD patients often suffer from gut microbiota dysbiosis, which mainly manifests as decreased microbial diversity and changes in microbial composition and microbiota‐derived metabolites, both of which are significant predictors of poor prognosis in GVHD patients. Therefore, the purpose of this review is to summarize what is known regarding changes in gut microbiota and microbiota‐derived metabolites in GVHD, their relationship to GVHD prognosis, and corresponding clinical strategies designed to prevent microbial dysregulation and facilitate treatment of GVHD.
Uncovering of transplanted connective tissue graft: Clinical and histological evaluation
Authors: Fratini Adriano , Mancini Leonardo , Liguori Margherita G , Gotti Stefano , Marchetti Enrico 
Published: January 2024

AbstractBackgroundPeriodontal plastic surgery aims to restore recessions and dehiscence around teeth and implants. Several techniques, such as subepithelial connective tissue graft (CTG), were proposed with the main outcome of improving volume and root coverage. Nevertheless, this surgery might not improve the keratinized tissue width. Thus, the primary aim of this case report was to describe the possible increase in keratinized tissue after a subepithelial CTG and simultaneously use the previously harvested graft as a source for covering an adjacent tooth.MethodsA 38‐year‐old patient presented brushing discomfort 2 years after undergoing periodontal plastic surgery with a CTG from the palate to cover a recession. Despite the increased thickness of the soft tissue, brushing discomfort was not reduced because the tissue quality remained unchanged. Therefore, a surgical procedure in the area of teeth 3.2–3.4 was performed to remove the more superficial masticatory mucosa and to induce keratinization of the previously grafted connective tissue.ResultsAfter 6 months, the epithelium appears to be clinically and histologically keratinized, with characteristics comparable to those of the original tissue.ConclusionsThe connective tissue grafted maintains the potential to induce keratinization over time, if it is exposed.Key points
Why is this case new information?To the best of our knowledge, this is the first case report in the literature with a histological evaluation in a human of the tissue grafted exposed after 2 years.

What are the keys to successful management of this case?The graft covered by the alveolar mucosa did not induce keratinization of the epithelium of the overlying mucosa.

What are the primary limitations to success in this case?The main limitation of this study is that it is a singular case report.
Algerian Propolis from Distinct Geographical Locations: Chemical Profiles, Antioxidant Capacity, Cytotoxicity and Inhibition of 5‐Lipoxygenase Product Biosynthesis
Authors: Ayad Ahmed Sabri , Hébert Mathieu P. A. , Doiron Jérémie A. , Loucif‐Ayad Wahida , Daas Tarek , Smagghe Guy , Alburaki Mohamed , Barnett David A. , Touaibia Mohamed , Surette Marc E. 
Published: January 2024

AbstractPropolis was collected from honeybee hives in three geographically distinct Algerian climates and extracts were characterized for composition and bioactivity. Bees were identified as native subspecies using an in‐silico DraI mtDNA COI‐COII test. Over 20 compounds were identified in extracts by LC‐MS. Extracts from the Medea region were more enriched in phenolic content (302±28 mg GAE/g of dry extract) than those from Annaba and Ghardaia regions. Annaba extracts had the highest flavonoid content (1870±385 mg QCE/g of dry extract). Medea extracts presented the highest free‐radical scavenging activity (IC50=13.5 μg/mL) using the DPPH radical assay while Ghardaia extracts from the desert region were weak (IC50>100 μg/mL). Antioxidant activities measured using AAPH oxidation of linoleic acid were similar in all extracts with IC50 values ranging from 2.9 to 4.9 μg/mL. All extracts were cytotoxic (MTT assay) and proapoptotic (Annexin‐V) against human leukemia cell lines in the low μg/mL range, although the Annaba extract was less active against the Reh cell line. Extracts inhibited cellular 5‐lipoxygenase product biosynthesis with IC50 values ranging from 0.6 to 3.2 μg/mL. Overall, examined propolis extracts exhibited significant biological activity that warrant further characterization in cellular and in vivo models.
Influence of Seed structure on Volume distribution of α‐Synuclein Oligomer at Early Stages of Aggregation using nanopipette
Authors: Charles‐Achille Saly , Janot Jean‐Marc , Cayrol Bastien , Balme Sebastien 
Published: January 2024

AbstractUnderstanding α‐synuclein aggregation is crucial in the context of Parkinson's disease. The objective of this study was to investigate the influence of aggregation induced by preformed seeding on the volume of oligomers during the early stages, using a label‐free, single‐molecule characterization approach. By utilizing nanopipettes of varying sizes, the volume of the oligomers can be calculated from the amplitude of the current blockade and pipette geometry. Further investigation of the aggregates formed over time in the presence of added seeds revealed an acceleration in the formation of large aggregates and the existence of multiple distinct populations of oligomers. Additionally, we observed that spontaneously formed seeds inhibited the formation of smaller oligomers, in contrast to the effect of HNE seeds. These results suggest that the seeds play a crucial role in the formation of oligomers and their sizes during the early stages of aggregation, whereas the classical thioflavin T assay remains negative.