bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guided Tissue Regeneration in Human Class II Furcation Defects Using a Diphenylphosphorylazide‐Cross‐Linked Collagen Membrane: A Consecutive Case Series
Authors: Zahedi Charles S. , Miremadi S. Asghar , Brunel Gérard , Rompen Eric , Bernard Jean‐Pierre , Benque Edmond 
Published: February 2005

Background: Several bioabsorbable collagen membranes are either currently available or under investigation for use in guided tissue regeneration (GTR) procedures. The aim of this 12‐month reentry study was to evaluate a diphenylphosphorylazide (DPPA)‐cross‐linked bovine type I collagen membrane in the healing of mandibular Class II furcation defects. The DPPA technique prevents the inclusion of foreign cytotoxic cross‐linking substances in the reticulated molecules, thus providing a more biocompatible product.Methods: Forty non‐smoking periodontally compromised adult subjects volunteered for this study, and one defect was randomly selected in each patient. At baseline, plaque index (PI), gingival index GI), probing depth (PD), gingival margin location GML), clinical attachment level (CAL), and width of the attached gingiva (AG) were measured. Intrasurgical hard tissue measurements consisted of the vertical open probing attachment level (V‐OPAL) and horizontal open probing attachment level (H‐OPAL), as well as furcation entrance width (FW) and height (FH) and subclassification of defects according to vertical bone loss. All soft tissue measurements were repeated 12 months after membrane placement using conservative flap reentry surgeries; hard tissue parameters were also assessed at this time.Results: DPPA‐cross‐linked collagen membranes demonstrated good handling characteristics, and no postoperative adverse tissue reactions or clinically detectable localized allergic responses were observed. GI and PI remained low during the entire study period. When the one year reentry values were compared to the baseline measurements, a significant reduction in PD (1.9 ± 1.4), FW (2.1 ± 1.7), FH (2.9 ± 1.6), and gain in CAL (1.7 ± 1.5), V‐OPAL (2.1 ± 1.9), and H‐OPAL (4.7 ± 1.4) were observed (P ≤0.05). However, there were no significant differences in GML or AG. Hard tissue improvements resulted in the complete closure of 30% (12) of the defects, and transformation of 67.5% (27) of the defects to Class I, while one defect remained a Class II (2.5%).Conclusions: Based on this 12‐month clinical reentry study, a DPPA‐cross‐linked collagen membrane could be beneficial in GTR treatment of Class II mandibular furcation involvements, both in terms of soft and hard tissue improvements. J Periodontol 2003;74:1071‐1079.
Changes in Alveolar Bone Height and Width Following Post‐Extraction Ridge Augmentation Using a Fixed Bioabsorbable Membrane and Demineralized Freeze‐Dried Bone Osteoinductive Graft
Authors: Zubillaga Gabriel , Von Hagen Stanley , Simon Barry I. , Deasy Michael J. 
Published: February 2005

Background: It has been shown that the amount of healed bone following guided bone regeneration (GBR) with demineralized freeze‐dried bone allograft (DFDBA) and a bioabsorbable membrane is significantly less than the initial quantity. A study was designed to determine if the amount of GBR would be affected by using an osteoinductive DFDBA and bioabsorbable membrane and membrane stabilization.Methods: Eleven extraction sites (10 patients) were treated with DFDBA and bioabsorbable membrane before placing endosseous implants. Standardized alveolar height and width measurements were taken after extraction, GBR, and 4 months postoperatively, at predetermined measurement points (sites midpoint and 3 mm mesial and distal from the midpoint) and classified as augmented (<1 mm increase of GBR height or width) or grafted (>1 mm increase). Five membranes were stabilized.Results: Three mm from the crest, augmented points exhibited a complete loss of augmented width. There was also some loss of pre‐GBR bone width (ranging from 4.7% to 20%) at augmented and grafted points. Five mm from the crest, augmented points lost 83.3% to 92.3% of augmented width and grafted points lost 12.9% to 18% of pre‐GBR width. Loss of augmented height ranged from 93.5% to 100%. Augmented (except distal) and grafted measurement points lost 2.1% to 12% of pre‐GBR height. Comparing tacked and non‐tacked sites, the former manifested less loss of augmented bone width, the latter augmented bone height.Conclusions: Results indicate a complete loss of augmented width 3 mm from the crest and almost complete loss in height and width 5 mm from the crest. Membrane stabilization appeared beneficial. J Periodontol 2003;74:965‐975.
Influence of Cyclosporin A Therapy on Bone Healing Around Titanium Implants: A Histometric and Biomechanic Study in Rabbits
Authors: Sakakura Celso E. , Margonar Rogério , Holzhausen Marinella , Nociti Francisco H. , Alba Rodolfo Candia , Marcantonio Elcio 
Published: February 2005

Background: Immunosuppressive agents may induce severe changes on bone metabolism. The purpose of the present study was to evaluate the influence of the administration of cyclosporin A (CsA) on the bone tissue around titanium implants.Methods: Eighteen New Zealand rabbits were randomly divided into 2 groups of 9 each. The test group (CsA) received daily subcutaneous injection of CsA (10 mg/kg body weight) and the control group (CTL) received saline solution by the same administration route. Three days after therapy began, 2 implants (7.0 mm long and 3.75 mm in diameter) were inserted bilaterally at the region of the tibial methaphysis. After 4, 8, and 12 weeks the animals were sacrificed and biomechanical tests and histometrical procedures, consisting of the determination of the percentages of bone‐implant contact and bone area within the limits of the implant threads, were performed.Results: Intergroup analysis showed that the removal torque and the percentage of bone contact with the implant surface for CsA group were significantly lower than those of the CTL group at 12 weeks (28.5 and 39.2 N cm, P = 0.01; 7.76% and 18.52%, P = 0.02, respectively).Conclusion: The data from the present study suggest that long‐term administration of cyclosporin A may negatively influence bone healing around dental implants. J Periodontol 2003;74:976‐981.
Human Periodontal Ligament Cell Responses to Recombinant Human Bone Morphogenetic Protein‐2 with and without Bone Allografts
Authors: Markopoulou C.E. , Vrotsos I.A. , Vavouraki H.N. , Dereka X.E. , Mantzavinos Z.S. 
Published: February 2005

Background: Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) has been found to promote the osteoblastic differentiation of human periodontal ligament cells. Its effect depends on the delivery system used. In this study we examined the effect of rhBMP‐2 on the proliferation and osteoblastic differentiation of human periodontal ligament cells cultured alone or with 3 different bone allografts.Methods: The rhBMP‐2 effect on cell proliferation and osteoblastic differentiation was examined by measuring [3H] thymidine incorporation and ALPase activity, respectively, on human periodontal ligament (hPDL) cells. Two human demineralized freeze‐dried allografts of cortical (DFDBAco) and cancellous (DFBDAca) bone origin and 1 non‐demineralized freeze‐dried allograft (FDBA) of cancellous bone origin, derived from different tissue banks, were used to evaluate the rhBMP‐ 2 effect on cell osteoblastic differentiation. The measurements were taken on various days.Results: rhBMP‐2 decreased hPDL cell proliferation. rhBMP‐ 2 acted on the third day of the process of cell differentiation, had a specific time of action, achieved its peak effect on the fourth and fifth days, and then did not provoke any further effects. The 3 bone allografts were efficiently combined with rhBMP‐2. The combination of rhBMP‐2 and DFDBAco showed the effect with the longest duration. rhBMP‐2, on day 4, made the inactive bone allograft more active while, on the other days, its effect was dependent on the allograft alone.Conclusions: rhBMP‐2 promotes the osteoblastic differentiation of human periodontal ligament cells and decreases cell proliferation. In this study rhBMP‐2 in the presence of the bone allografts tested resulted in hPDL cell differentiation. J Periodontol 2003;74:982‐989.
Comparison of Porous and Non‐Porous Teflon Membranes Plus a Xenograft in the Treatment of Vertical Osseous Defects: A Clinical Reentry Study
Authors: Walters Stephen P. , Greenwell Henry , Hill Margaret , Drisko Connie , Pickman Kim , Scheetz James P. 
Published: February 2005

Background: The primary aim of this 9‐month randomized, controlled, blinded, clinical reentry study was to compare the regenerative effects of a nonporous polytetrafluoroethylene (NP) periodontal membrane to a porous expanded polytetrafluoroethylene (P) periodontal membrane in the treatment of vertical osseous defects.Methods: Twenty‐four patients, 11 males and 13 females, age 24 to 74 (mean 50.5 ± 13.1) provided one site with an intraosseous defect ≥4 mm and were divided equally and randomly into two groups. Following debridement both groups were grafted with a bovine‐derived xenograft coated with a synthetic cell‐binding peptide; then the test group received an NP membrane and the control group received a P membrane. All defects were reentered after 9 months. Measurements were performed by a masked examiner.Results: There were no statistically significant differences (P >0.05) between NP and P groups for any open or closed probing measurement at any time. Similar open initial defect depth for the NP group and P groups (4.8 versus 5.0 mm) demonstrated identical 9‐month defect fill of 2.8 mm (57%) for both groups. A difference in crestal resorption for the NP compared to the P group (0.4 versus 0.8 mm) accounted for the difference in mean percent defect resolution, which was 67% for NP compared to 72% for the P group. Overall, nine (75%) of the NP group defects and eight (67%) of the P group defects showed more than 50% defect fill.Conclusion: Treatment of vertical osseous defects with nonporous or porous polytetrafluoroethylene membranes in combination with a xenograft resulted in statistically signidficant improvement in open and closed probing measurements, with no significant difference between treatment groups. J Periodontol 2003;74:1161‐1168.
Role of Insulin‐Like Growth Factor‐1 Signaling in Dental Fibroblast Apoptosis
Authors: Han Xiaozhe , Amar Salomon 
Published: February 2005

Background: Studies have shown that periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) respond differently to growth factors in the repair and regeneration of periodontal tissues. The goal of this study was to determine the effects of insulin‐like growth factor‐1 (IGF‐1) signaling on cell apoptosis in PDLF compared to GF.Methods: The levels of apoptosis were compared between cultured PDLF and GF by DNA fragmentation assay and trypan blue exclusion assay, either in the presence or absence of IGF‐1. The transcript level of upstream signaling molecules, such as IGF binding protein‐5 (IGFBP‐5), IGF‐1 receptor (IGF‐1R), and phosphoinositide 3‐kinase (PI3K), was studied using reverse transcription‐ polymerase chain reaction (RT‐PCR). Furthermore, the role of IGFBP‐5 in IGF‐1 signaling was verified by annexin‐V staining using ﬂow cytometric analysis.Results: IGF‐1 significantly inhibited the level of DNA fragmentation and decreased trypan blue‐positive cells in PDLF compared to GF during serum deprivation. The mRNA expression of IGFBP‐5, IGF‐1R, and PI3K was constitutively upregulated in PDLF compared to GF. In the presence of exogenous IGFBP‐5, the annexin‐V–positive cells were significantly decreased in GF after IGF‐1 stimulation.Conclusions: The present study provides evidence that IGF‐1 reduces apoptosis in cultured PDLF compared to GF. Upregulation of IGF‐1R and PI3K in PDLF further suggests the activation of IGF signaling in PDLF. In addition, the anti‐apoptotic effect of IGF‐1 may be facilitated by the upregulation of IGFBP‐5 in PDLF. J Periodontol 2003;74:1176‐1182.
Association Between Interleukin‐1 Genotype and Periodontal Disease in a Diabetic Population
Authors: Guzman Sergio , Karima Mamdouh , Wang Hwa‐Ying , Van Dyke Thomas E. 
Published: February 2005

Background: Recently, it has become evident that for many common chronic diseases, modifying factors amplify disease mechanisms to make the clinical condition more severe. The aims of this report were 1) to investigate the prevalence of periodontitis in a diabetic population, 2) to evaluate the association of periodontitis with metabolic control, and 3) to evaluate periodontitis in diabetics with different interleukin (IL)‐1 genotypes.Methods: One hundred diabetic patients were screened. Type and duration of diabetes, level of control (glycosylated hemoglobin), and demographic data were recorded. Periodontal disease was defined as two or more teeth with clinical attachment loss (CAL) ≥5 mm. Poorly controlled diabetes was defined as glycosylated hemoglobin values >8%. Finger‐stick blood samples were collected and analyzed for genotyping of IL‐1A (+4845), IL‐1B (+3954), IL‐1B (–511), and IL‐1RN (+2018) polymorphisms.Results: Among the diabetic patients in the study, 66% showed periodontal destruction, and 43% of those could be characterized as severe. The prevalence of severe attachment loss increased with decreasing control of diabetes. Only the IL‐1B (–511) genotype was found to be associated with periodontal disease in the African American patients (P <0.05). The frequency of allele 1 was 0.77 in periodontitis affected versus 0.33 in healthy African American diabetics. A borderline significant association between IL‐1B (+3954) and periodontal disease also was noted in Caribbean periodontal patients (P = 0.06); however, the allele 2 frequency in this population was only 10%.Conclusions: These data confirm the high prevalence and severity of periodontitis in the diabetic population, and support the association between poor glycemic control and periodontal disease. The low prevalence of some of the IL‐1 gene polymorphisms in the ethnic groups included in this study limits the validity of conclusions on genotype associations with clinical findings, but there was a trend suggesting that allele 1 at IL‐1B (–511) and IL‐1B (+3954) was overrepresented among diabetics with periodontal disease. J Periodontol 2003;74:1183‐1190.
Effects of Enamel Matrix Derivative on Porphyromonas gingivalis
Authors: Newman Seth A. , Coscia Salvatore A. , Jotwani Ravi , Iacono Vincent J. , Cutler Christopher W. 
Published: February 2005

Background: Enamel matrix derivative (EMD) is used during periodontal surgery for the regeneration of periodontal tissue. It consists of the amelogenin fraction of porcine enamel matrix (AMEL) suspended in a vehicle of propylene glycol alginate (PGA). EMD‐treated sites appear to heal with less inﬂammation. It has been suggested that antimicrobial properties of EMD might account for improved healing in vivo. The objectives of this study were: 1) to determine the antibacterial effects of EMD on the periodontal pathogen Porphyromonas gingivalis and 2) to establish the component(s) of EMD that are responsible for this effect.Methods: The antimicrobial effects were determined in vitro using the broth dilution assay. P. gingivalis at a starting inoculum of 109 colony forming units/ml was treated with EMD, AMEL, and PGA in Hank's balanced salt solution (HBSS) for 1, 3, and 24 hours. The CFU/ml of P. gingivalis recovered on enriched Brucella blood agar was determined at 6 and 10 days.Results: EMD (containing AMEL and PGA) or PGA alone eliminated recoverable CFUs of P. gingivalis. Interestingly, AMEL in HBSS increased recoverable CFUs from 8.62 log CFU/ml to 8.93 log CFU/ml. Further analysis of the dose response at concentrations of 0.3, 3, and 30 mg/ml of AMEL in HBSS revealed that only 30 mg/ml (clinical concentration) increased CFUs of P. gingivalis relative to baseline (from 8.8 log CFU/ml to 9.2 log CFU/ml in 3 hours). Additionally, AMEL was compared to a protein control bovine serum albumin (BSA) to determine whether this effect was unique to AMEL. A marked increase in recoverable CFUs occurred with the AMEL (increasing from 8.8 log CFU/ml to 9.47 log CFU/ml), but not with BSA.Conclusions: EMD possesses antimicrobial properties that can be attributed to the propylene glycol alginate vehicle. The amelogenin fraction of porcine enamel matrix in enamel matrix derivative (i.e., AMEL) is not antibacterial for P. gingivalis and was shown to increase recoverable CFUs.J Periodontol 2003; 74:1191‐1195.
Comparative Clinical Study of Connective Tissue Graft and Two Types of Bioabsorbable Barriers in the Treatment of Localized Gingival Recessions
Authors: Çetiner Deniz , Parlar Ateş , Baloş Köksal , Alpar Reha 
Published: February 2005

Background: Localized buccal recessions occur in more than 60% of individuals; therefore, there is a need for predictable root coverage techniques. The objective of the present study was to evaluate the clinical effectiveness of the guided tissue regeneration (GTR) procedure versus connective tissue graft (CTG) in the treatment of localized gingival recessions over a 12‐month postoperative period.Methods: Thirty bilateral matched pairs of localized buccal recessions in 22 patients were treated with CTG and GTR in this study. For the GTR procedure, two types of bioabsorbable barriers, polylactide/polyglycolide acid (PLGA) and solvent dehydrated duramater allograft (SDDA) membranes, were used and CTG was obtained from the palatal mucosa. The selected pairs of teeth were randomly assigned to one of three groups (10 pairs per group): group 1, PLGA (10 recessions) or CTG (10 recessions); group 2, SDDA (10 recessions) or CTG (10 recessions); or group 3, PLGA (10 recessions) or SDDA (10 recessions). Statistical analysis evaluated both intra‐ and intergroup measurements. The height of gingival recession (GR), width of keratinized tissue (KT), clinical attachment level (CAL), and probing depth (PD) were assessed at baseline and at 6, 9, and 12 months following surgery.Results: The amount of root coverage was 74.3%, 69.6%, and 86.3% with PLGA, SDDA, and CTG, respectively. The gain in KT was significant in the CTG group (P <0.05). No significant differences were observed among the three groups with respect to PD and CAL.Conclusions: Results of this study indicate that all techniques led to an improvement of all clinical parameters except PD from baseline. However, CTG increased KT considerably compared to GTR. The final esthetic results were similar for the two membranes and connective tissue graft. J Periodontol 2003;74: 1196‐1205.
Effect of Hydroxamic Acid‐Based Matrix Metalloproteinase Inhibitors on Human Gingival Cells and Porphyromonas gingivalis
Authors: Yoshioka Masami , Yokoyama Nozomi , Masuda Kaname , Honna Tomoaki , Hinode Daisuke , Nakamura Ryo , Rouabhia Mahmoud , Mayrand Denis , Grenier Daniel 
Published: February 2005

Background: Matrix metalloproteinases (MMPs) are considered to play key roles in tissue destruction during periodontitis. In this study, we evaluated the cytotoxicity of hydroxamic acid‐based MMP inhibitors (ONO‐4817, ONO‐MI1‐514, and ONO‐MI1‐570), and their inhibitory effects on MMP‐2 and ‐9 activities and growth of Porphyromonas gingivalis.Methods: Human gingival fibroblasts (HGF) and human gingival epithelial cells (HGE) were incubated with test inhibitors prior to investigating cell viability, cell proliferation, and mRNA expression for MMP‐2 and ‐9. Gelatin zymography and a colorimetric MMP assay were performed to study the inhibitory effects on MMP‐2 and ‐9 activities derived from HGF and HGE, respectively. The effect of MMP inhibitors on keratinocyte migration and P. gingivalis growth was also tested.Results: Cell viability was not affected by any of the inhibitors at a final concentration of 50 µM, nor was cell proliferation at 20 µM. All inhibitors clearly inhibited MMP‐2 produced by HGF and MMP‐9 produced by HGE in a dose‐dependent manner. No change was found in mRNA expression of MMPs by gingival cells treated with the inhibitors. ONO‐4817 and ONO‐MI1‐514 inhibited keratinocyte migration. ONO‐4817 showed a slightly inhibitory effect on the growth of P. gingivalis.Conclusion: Data obtained in this study support the potential use of the three MMP inhibitors for the prevention and treatment of periodontal disease. J Periodontol 2003;74:1219‐1224.
A Case of Zimmermann‐Laband Syndrome with Supernumerary Teeth
Authors: Holzhausen Marinella , Gonçalves Daniela , Corrêa Fernanda de Oliveira Bello , Spolidorio Luís Carlos , Rodrigues Valter Curi , Orrico Silvana Regina Perez 
Published: February 2005

Background: Zimmermann‐Laband syndrome is a rare autosomal dominant disorder that is characterized by gingival fibromatosis, ear, nose, bone, and nail defects, and hepatosplenomegaly.Methods: This case report describes the clinical presentation and periodontal findings in a 13‐year old female patient with previously undiagnosed Zimmermann‐Laband syndrome.Results: Clinical and radiographic findings and genetic counseling confirmed the diagnosis of Zimmermann‐Laband syndrome. The most striking oral findings were the presence of gingival enlargement involving both the maxillary and mandibular arches, anterior open bite, non‐erupted teeth, and two supernumerary teeth. Periodontal treatment consisted of gingivectomy in four quadrants. Histopathologic evaluation of excised tissue supported the diagnosis of gingival fibromatosis. The patient was referred for appropriate orthodontic treatment and genetic counseling, and has been closely followed for the earliest signs of hepatosplenomegaly.Conclusions: Dental practitioners should be alert for developmental abnormalities that may occur in patients with gingival fibromatosis as this may indicate the presence of a rare disorder like Zimmermann‐ Laband syndrome. A comprehensive medical history and physical systemic evaluation are essential for correct diagnosis and treatment of these cases. J Periodontol 2003;74:1225‐1230.