bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens
Authors: Gao Mingkun , Liao Chaoyong , Fu Jianyang , Ning Zhonghua , Lv Zengpeng , Guo Yuming 
Published: February 2024

Background
Baicalin and probiotic cocktails are promising feed additives with broad application prospects. While probiotic cocktails are known to enhance intestinal health, the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored. Therefore, this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the health-promoting effects.

Results
A total of 320 1-day-old male Arbor Acres broilers were divided into 4 groups, each with 8 replicates of 10 chicks per replicate. Over a period of 42 d, the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin (BC), 1,000 g/t probiotic cocktails (PC), or a combination of both BC (37.5 g/t) and PC (1,000 g/t). The results demonstrated that BC + PC exhibited positive synergistic effects, enhancing intestinal morphology, immune function, and barrier function. This was evidenced by increased VH/CD ratio, sIgA levels, and upregulated expression of occludin and claudin-1 (P < 0.05). 16S rRNA analysis indicated that PC potentiated the effects of BC, particularly in the ileum, where BC + PC significantly increased the α-diversity of the ileal microbiota, altered its β-diversity, and increased the relative abundance of Flavonifractor (P < 0.05), a flavonoid-metabolizing bacterium. Furthermore, Flavonifractor positively correlated with chicken ileum crypt depth (P < 0.05). While BC + PC had a limited effect on cecal microbiota structure, the PC group had a very similar microbial composition to BC + PC, suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.

Conclusions
We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails. Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum, thereby influencing the ileal microbiota structure. This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota. These findings provide compelling evidence for the future development of feed additive combinations.
Prevotella and succinate treatments altered gut microbiota, increased laying performance, and suppressed hepatic lipid accumulation in laying hens
Authors: Liu Min , Kang Zeyue , Cao Xikang , Jiao Hongchao , Wang Xiaojuan , Zhao Jingpeng , Lin Hai 
Published: February 2024

Abstract
Background
This work aimed to investigate the potential benefits of administering Prevotella and its primary metabolite succinate on performance, hepatic lipid accumulation and gut microbiota in laying hens.

Results
One hundred and fifty 58-week-old Hyline Brown laying hens, with laying rate below 80% and plasma triglyceride (TG) exceeding 5 mmol/L, were used in this study. The hens were randomly allocated into 5 groups and subjected to one of the following treatments: fed with a basal diet (negative control, NC), oral gavage of 3 mL/hen saline every other day (positive control, PC), gavage of 3 mL/hen Prevotella melaninogenica (107 CFU/mL, PM) or 3 mL/hen Prevotella copri (107 CFU/mL, P. copri) every other day, and basal diet supplemented with 0.25% sodium succinate (Succinate). The results showed that PM and P. copri treatments significantly improved laying rate compared to the PC (P < 0.05). The amount of lipid droplet was notably decreased by PM, P. copri, and Succinate treatments at week 4 and decreased by P. copri at week 8 (P < 0.05). Correspondingly, the plasma TG level in Succinate group was lower than that of PC (P < 0.05). Hepatic TG content, however, was not significantly influenced at week 4 and 8 (P > 0.05). PM treatment increased (P < 0.05) the mRNA levels of genes PGC-1β and APB-5B at week 4, and ACC and CPT-1 at week 8. The results indicated enhanced antioxidant activities at week 8, as evidenced by reduced hepatic malondialdehyde (MDA) level and improved antioxidant enzymes activities in PM and Succinate groups (P < 0.05). Supplementing with Prevotella or succinate can alter the cecal microbiota. Specifically, the abundance of Prevotella in the Succinate group was significantly higher than that in the other 4 groups at the family and genus levels (P < 0.05).

Conclusions
Oral intake of Prevotella and dietary supplementation of succinate can ameliorate lipid metabolism of laying hens. The beneficial effect of Prevotella is consistent across different species. The finding highlights that succinate, the primary metabolite of Prevotella, represents a more feasible feed additive for alleviating fatty liver in laying hens.
Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function
Authors: Kang Tae Hong , Lee Sang In 
Published: February 2024

Abstract
Background
Deoxynivalenol (DON) is a mycotoxin that has received recognition worldwide because of its ability to cause growth delay, nutrient malabsorption, weight loss, emesis, and a reduction of feed intake in livestock. Since DON-contaminated feedstuff is absorbed in the gastrointestinal tract, we used chicken organoids to assess the DON-induced dysfunction of the small intestine.

Results
We established a culture system using chicken organoids and characterized the organoids at passages 1 and 10. We confirmed the mRNA expression levels of various cell markers in the organoids, such as KI67, leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5), mucin 2 (MUC2), chromogranin A (CHGA), cytokeratin 19 (CK19), lysozyme (LYZ), and microtubule-associated doublecortin-like kinase 1 (DCLK1), and compared the results to those of the small intestine. Our results showed that the organoids displayed functional similarities in permeability compared to the small intestine. DON damaged the tight junctions of the organoids, which resulted in increased permeability.

Conclusions
Our organoid culture displayed topological, genetic, and functional similarities with the small intestine cells. Based on these similarities, we confirmed that DON causes small intestine dysfunction. Chicken organoids offer a practical model for the research of harmful substances.
Accuracy and precision of dried urine spot method for the detection of Schistosoma mansoni circulating cathodic antigens in resource-limited settings
Authors: Zacharia Abdallah , Kinabo Clemence , Makene Twilumba , Omary Huda , Ogweno George , Lyamuya Faraja , Ngasala Billy 
Published: February 2024

AbstractBackgroundThe World Health Organization recommends the use of Schisto point-of-care circulating cathodic antigens (Schisto POC-CCA) for screening ofSchistosoma mansonias it offers better sensitivity than microscopy. However, there are limitation facing the use of this method including timely availability of the test cassettes. The aim of this study was to determine the reliability of dried urine spot (DUS) method for collection of urine and detection ofS. mansoniusing Schisto POC-CCA cassettes in a resource-limited settings.MethodsA cross-sectional study was conducted between October and November 2022 among 250 primary school children in Sengerema District, northwestern Tanzania.S. mansoniCCA was detected in filter paper-based DUS, liquid urine using DUS Schisto POC-CCA (index), and direct urine Schisto POC-CCA (comparator) methods respectively.S. mansonieggs in stool were detected using duplicate Kato-Katz (KK) method. The measures of accuracy were computed and compared between the index and comparator methods. The strength of agreement between inter-raters precisions was tested using Cohen’s kappa (k).ResultsThis study revealedS. mansoniprevalence rates of 28.8%, 54.0% and 50.8% by duplicate KK, direct urine Schisto POC-CCA and DUS Schisto POC-CCA methods respectively. The mean intensity of infection among infected participants was 86.3 eggs per gram of stool (EPG) ranging from 12.0 EPG to 824.0 EPG. The sensitivity of DUS Schisto POC-CCA and direct urine Schisto POC-CCA was 94.44% (95%CI: 89.15–99.74%) and 97.22% (95%CI: 93.43–100.00%) respectively. The DUS Schisto POC-CCA method had slightly higher specificity (66.85%) than direct urine Schisto POC-CCA method (63.48%). The accuracy of the DUS Schisto POC-CCA was found to be slightly high (74.80%, 95%CI: 68.94–79.06%) compared to that of direct urine Schisto POC-CCA (73.20%, 95%CI: 67.25–78.59%). There was good agreement between two laboratory technologists who performed the DUS Schisto POC-CCA method on similar samples (k = 0.80, 95%CI: 0.59–0.95).ConclusionsThe DUS Schisto POC-CCA method had comparableS. mansonidetection accuracy to direct urine Schisto POC-CCA. This suggests that the method could be a potential alternative to direct urine Schisto POC-CCA for screeningS. mansoniin resource-limited situations.Graphical Abstract
Unsuccessful tuberculosis treatment outcomes across Brazil's geographical landscape before and during the COVID-19 pandemic: are we truly advancing toward the sustainable development/end TB goal?
Authors: Tavares Reginaldo Bazon Vaz , Berra Thaís Zamboni , Alves Yan Mathias , Popolin Marcela Antunes Paschoal , Ramos Antônio Carlos Vieira , Tártaro Ariela Fehr , de Souza Clara Ferreira , Arcêncio Ricardo Alexandre 
Published: February 2024

Abstract
Background
Tuberculosis is one of the most significant infectious diseases for global public health. The reallocation of healthcare resources and the restrictions imposed by the COVID-19 pandemic have hindered access to TB diagnosis and treatment. Increases in unfavorable outcomes of the disease have been observed in Brazil. The objective of this study was to analyze the spatial distribution of unfavorable TB treatment outcomes in Brazil before and during the pandemic.

Methods
An ecological study with spatial analysis was conducted with all 5569 municipalities in Brazil. All reported cases of tuberculosis between January 2010 and December 2021, as well as reported cases of COVID-19 from February 2020 to December 2021, were included. The outcomes studied encompass loss to follow-up, drug-resistant tuberculosis, and death. The Getis Ord GI* technique was employed to assess spatial association, and the Kernel density estimator was used to identify areas with concentrated increases or decreases in outcomes. Bivariate Local Moran's I was used to examine the spatial association between outcomes and COVID-19 incidence. The study was approved by the Research Ethics Committee of Ribeirão Preto Nursing School, University of São Paulo.

Results
There were 134,394 cases of loss to follow-up, 10,270 cases of drug resistance, and 37,863 deaths. Clusters of high and low values were identified for all three outcomes, indicating significant changes in the spatial distribution patterns. Increases in concentrations were observed for lost to follow-up cases in the Southeast, while reductions occurred in the Northeast, South, and Midwest. Drug-resistant tuberculosis experienced an increase in the Southern and Southeastern regions and a decrease in the Northeast and South. TB-related deaths showed notable concentrations in the Midwest, Northeast, South, and Southeast. There was an increase in high occurrence clusters for deaths after 2020 and 2021 in the Northeast.

Conclusions
The pandemic has brought additional challenges, emphasizing the importance of enhancing efforts and disease control strategies, prioritizing early identification, treatment adherence, and follow-up. This commitment is vital for achieving the goal of tuberculosis elimination.
Effectiveness of ivermectin mass drug administration in the control of soil-transmitted helminth infections in endemic populations: a systematic review and meta-analysis
Authors: Le Brandon , Clarke Naomi E. , Legrand Nicolas , Nery Susana Vaz 
Published: February 2024

Abstract
Background
Current soil-transmitted helminth (STH) control guidelines endorse the use of albendazole or mebendazole for school-based targeted preventive chemotherapy (PC), yet their reduced efficacy against Strongyloides stercoralis and Trichuris trichiura presents significant limitations. Emerging evidence indicates that community-wide PC [or mass drug administration (MDA)] using ivermectin, commonly used in other neglected tropical disease (NTD) control programs, may play an important role in controlling these parasites. We conducted a systematic review and meta-analysis to evaluate the effectiveness of ivermectin PC in reducing STH prevalence in endemic populations.

Methods
We searched Pubmed, EMBASE, and Web of Science on February 14, 2023, for studies that investigated the effectiveness of ivermectin PC, either alone or in combination with other anthelmintic drugs, on STH infections, and provided a measure of STH prevalence before and after PC. We calculated pooled prevalence reductions for each STH using random-effects meta-analyses. Our protocol is available on PROSPERO (registration number CRD42023401219).

Results
A total of 21 were eligible for the systematic review, of which 15 were eligible for meta-analysis. All studies delivered ivermectin through MDA. The pooled prevalence reduction of S. stercoralis following MDA with ivermectin alone was 84.49% (95% CI 54.96–94.66) across five studies and 81.37% (95% CI 61.62–90.96) across seven studies with or without albendazole. The prevalence reduction of T. trichiura was 49.93% (95% CI 18.23–69.34) across five studies with ivermectin alone, and 89.40% (95% CI 73.66–95.73) across three studies with the addition of albendazole. There was high heterogeneity for all syntheses (I2 > 65%).

Conclusions
This study underscores the key role of ivermectin-based MDA in addressing limitations in current global STH guidelines in terms of limited efficacy against S. stercoralis and T. trichiura. Based on these findings, revising international STH guidelines to include ivermectin is a promising option to progress the control and eventual elimination of STHs and other NTDs.
Application of the on-site P-wave earthquake early warning method based on site-specific ratios of S-waves to P-waves to the 2016 Kumamoto earthquake sequence, Japan
Authors: Tsuno Seiji , Niwa Katsutomo , Korenaga Masahiro , Yamanaka Hiroaki , Chimoto Kosuke , Miyake Hiroe , Yamada Nobuyuki 
Published: February 2024

AbstractThe on-site P-wave earthquake early warning (EEW) based on the site-specific ratios of S-waves to P-waves has been applied to large-sized offshore earthquakes, and the efficiency of the method has been validated. However, the method requires the P-waves including earthquake ground motions radiated from a large slip area while avoiding the inclusion of S-waves. In this study, we investigated the applicability of the on-site P-wave EEW method for ground motions near an earthquake source fault region, using strong-motion data observed during the 2016 Kumamoto earthquake sequence in Japan. At first, we examined the appropriate time-window length following the arrival of the P-waves. As a result, P-waves with a time-window length of 2.56 s after the arrival at most strong-motion stations were required at least to predict appropriately S-waves for the 2016 Kumamoto earthquake sequence, including the large-sized earthquakes. On the other hand, in the case of the large-sized earthquake as the mainshock (Mj 7.3), the method can predict within a brief time of 0.5 to 2 s in the operational use that strong ground motions exceeding a certain threshold (e.g., acceleration of 150 cm/s2) will come. Moreover, we found that the method was not strongly affected by the non-linearity of soil deposits due to strong ground motions during the 2016 Kumamoto earthquake sequence. The variability of the relationship between P- and S-waves at the seismic bedrock influenced by the source and path effects is larger than the variability of the relationships between P-/S-waves at the seismic bedrock and at the ground surface by the site effects, and therefore, it hides the effect of the non-linearity of soil deposits.
Graphical Abstract
Mid-infrared imaging spectroscopic measurements of C2H4 frost simulating the outer solar system environments
Authors: Koga Ryoichi , Negishi Shohei , Zhao Biao , Li Yuan , Ito Fumiyuki , Kasaba Yasumasa , Hirahara Yasuhiro 
Published: February 2024

AbstractIn the dense and cold atmosphere of Titan, the presence of C2H4 haze has been confirmed by the observations of spacecraft. In the present study, original cryogenic experimental equipment was developed to simulate the low-temperature solid formation of C2H4 in combination with in-situ infrared spectroscopic measurements. As a result, out-of-plane bending vibration ν7 of solid-phase C2H4 located at ~ 10.5 μm was successfully detected with high sensitivity, and two-dimensional spectrographs of C2H4 at low temperatures were obtained. The obtained spectra of C2H4 can be fitted to the double Lorentzian function with various heights, central wavelengths, and full widths at half the maximum (FWHM) of the two-component Lorentzian functions. They were classified into three types using the fitting parameters. However, their spectral shapes are different from the amorphous, metastable, and crystalline forms obtained by the previous laboratory experiment in terms of the distance of two peak wavelengths and FWHM. The results may link to understanding the spectral band properties of C2H4 condensation in the haze component of Titan.
Graphical Abstract
Biomaterial-based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration
Authors: Li Ying-Ying , Ji Shuai-Fei , Fu Xiao-Bing , Jiang Yu-Feng , Sun Xiao-Yan 
Published: February 2024

AbstractScar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration, promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions. The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties. However, a comprehensive understanding of the underlying mechanisms remains somewhat elusive, limiting the broader application of these innovations. In this review, we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin. The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration. The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity, facilitating efficient cellular reprogramming and, consequently, promoting the regeneration of skin appendages. In summary, the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing, coupled with the restoration of multiple skin appendage functions.
Retrograde drainage for duodenal stump leakage using ileal decompression tube guided by double-balloon endoscopy: a novel case report
Authors: Naito Ryozan , Nakazawa Nobuhiro , Zennyoji Dan , Shimizu Takehiro , Hosoi Nobuhiro , Watanabe Takayoshi , Shioi Ikuma , Shibasaki Yuta , Osone Katsuya , Okada Takuhisa , Shiraishi Takuya , Sano Akihiko , Sakai Makoto , Ogawa Hiroomi , Sohda Makoto , Uraoka Toshio , Shirabe Ken , Saeki Hiroshi 
Published: February 2024

Abstract
Background
Duodenal stump leakage is a serious post-gastrectomy complication, and there have been no reports on endoscopic drainage.

Case presentation
We report a case of duodenal stump leakage after laparoscopic gastrectomy with Roux-en-Y reconstruction in a 68-year-old man. First-line conservative management was ineffective. Reoperation was performed because of severe abdominal pain and increased ascites. After reoperation, duodenal stump leakage recurred with bleeding from the anterior superior pancreaticoduodenal artery. Coil embolization and pigtail catheter insertion were performed. Furthermore, we retrogradely inserted an ileal tube for tube decompression near the duodenal stump using double-balloon endoscopy for effective drainage. After tube insertion, duodenal stump leakage decreased; on the 47th primary postoperative day, the patient was discharged. The primary postoperative course was uneventful after 1 year and 9 months of follow-up.

Conclusions
This is the first successful case of duodenal stump leakage treated with retrograde decompression tube insertion near the duodenal stump using double-balloon endoscopy.
Comparison of luteal phase stimulation with follicular phase stimulation in poor ovarian response: a single-blinded randomized controlled trial
Authors: Dastjerdi Mozhgan Vahabi , Ansaripour Soheila , Ataei Mina , Gharedaghi Roya , Hoseini Seyedeh Melika Mostafavi , Mohazzab Arash , Zafardoust Simin 
Published: February 2024

Abstract
Background
In the last decade, luteal-phase ovarian stimulation (LPOS) has been suggested as an alternative controlled ovarian stimulation (COS) protocol for in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles mainly in women with a history of poor ovarian response (POR). The present randomized controlled trial study aimed to compare the outcomes of follicular phase ovarian stimulation (FPOS) and LPOS protocols in POR cases undergoing ICSI cycles.

Methods
Seventy-eight POR patients who met the Bologna criteria and underwent an ICSI cycle were included. In this study, 39 POR cases were allocated to the FPOS group, and 39 POR cases were allocated to the LPOS group. The primary outcome was the number of metaphase II (MII) oocytes. In addition, the total number of oocytes, number of top-quality day 3 embryo, day 3 embryo development rate, chemical pregnancy and clinical pregnancy rates were defined as secondary outcomes.

Results
The obtained results demonstrated that the number of MII oocytes significantly increased in the LPOS group compared to the FPOS group (P = 0.007). However, there was no significant difference between the two groups regarding the number of GV and MI oocytes, number of top-quality day 3 embryos and day 3 embryo development rate among both categories of patients. Also, the number of total and MII oocytes was significantly higher in the LPOS group (P = 0.016).

Conclusion
These results suggest that LPOS protocol effectively increases the number of mature oocytes in women with a history of POR.

Trial registration
IRCT20210405050852N1 (Registered at Iranian registry of clinical trials; available at https://en.irct.ir/trial/55402).