bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Contribution of itlos to Fight Climate Change: Prospects and Challenges of the cosis Request for an Advisory Opinion
Authors: Mingozzi Pierre Clément 
Published: November 2023

Abstract
On 12 December 2022, the International Tribunal for the Law of the Sea (itlos) received a request from the Commission of Small Island States on Climate Change and International Law (cosis) to render an advisory opinion in order to clarify the obligations of States under the 1982 United Nations Convention on the Law of the Sea (unclos) to protect and preserve the marine environment in relation to climate change, including ocean warming, sea level rise, and ocean acidification. Although itlos has dealt with environment-related issues in the past, it has not yet specifically dealt with climate change and its (in)direct impacts. This contribution, drawing on the first opinion delivered by the Tribunal in 2015, aims to scrutinize some critical legal questions that the request will inevitably face, engaging particularly with its main procedural and substantive aspects. Since it is unlikely that itlos will encounter any unsurmountable obstacles to render the opinion, it would be expected that the Tribunal will take this opportunity to shed light on the implications of climate change for the legal regime of the sea, but also on the role of the Tribunal itself, to definitively clarify its advisory function.
The itlos Jurisprudence Regarding the Procedural Obligation to Conduct an Environmental Impact Assessment and Its Significance for Deep Seabed Mining
Authors: Tilloy Julio Alberto 
Published: November 2023

Abstract
The present work deals with the contribution of the International Tribunal for the Law of the Sea to the interpretation of the obligation to conduct an environmental impact assessment. Against this backdrop, the article pursues a twofold objective. On the one hand, it reinforces the idea that the case law of the Tribunal influenced ongoing regulating initiatives in the legal regime governing marine areas beyond national jurisdiction. On the other hand, considering the possible commencement of deep seabed mining activities in the Area, the article aims to demonstrate that the Tribunal could further clarify the legal basis of the eia obligation for exploitation purposes by building on its jurisprudence. This topic becomes particularly relevant in light of the “two-year rule” housed in Section 1(15) of the Annex to the 1994 Implementation Agreement and the absence of comprehensive and robust regulations.
Evaluation of Soil Moisture in the Canadian Seasonal to Interannual Prediction System, Version 2.1 (CanSIPSv2.1)
Authors: Sospedra-Alfonso Reinel , Merryfield William J. , Kharin Viatsheslav V. , Lee Woo-Sung , Lin Hai , Diro Gulilat T. , Muncaster Ryan 
Published: November 2023

Abstract
We evaluate the soil moisture hindcasts and the reconstruction runs giving the hindcasts initial conditions in version 2.1 of the Canadian Seasonal to Interannual Prediction System (CanSIPSv2.1). Different strategies are used to initialize the hindcasts for the two CanSIPSv2.1 models, CanCM4i and the coupled Global Environmental Multiscale, version 5.1, (GEM5)–NEMO model (GEM5-NEMO), with contrasting impacts on the soil moisture initial conditions and forecast performance. Forecast correlation skill is decomposed into contributions from persistence of the initial anomalies and contributions not linked to persistence, with performance largely driven by the accuracy of the initial conditions in regions of strong persistence. Seasonal soil moisture correlation skill is significant for several months into the hindcasts depending on initial and target months, with contributions not linked to persistence becoming more notable at longer lead times. For the first 2–4 months, the quality of CanSIPSv2.1 ensemble mean forecasts tends to be higher on average during summer and fall and is comparable to that of the best performing model, whereas CanSIPSv2.1 outperforms the single models during spring and winter. For longer lead times, remote climate influences from the Pacific Ocean are notable and contribute to predictable soil moisture variability in teleconnected regions.
Hyperlactatemia in patients undergoing pulmonary endarterectomy with deep hypothermic circulatory arrest: Risk factors and its effects on the outcome
Authors: Fang Ying-hui , Zhang Ya-jun , Zhen Ya-nan , Liu Xiao-peng , Sun Guang , Han Yong-xin 
Published: November 2023

Introduction To determine the risk factors of hyperlactatemia in pulmonary endarterectomy (PEA) surgery and assess whether elevated blood lactate levels are associated with adverse outcomes. Methods In this retrospective observational study, a total of 111 consecutive patients who underwent PEA for chronic thromboembolic pulmonary hypertension at the XXX Hospital between December 2016 and January 2022 were included. We retrospectively evaluated arterial blood samples analyzed intraoperatively. The pre- and intraoperative risk factors for hyperlactatemia and the postoperative outcomes were recorded. Results Lactate levels gradually increased during surgery. The optimal cut-off lactate level for major postoperative complications, calculated using receiver operating characteristic analysis, was 7.0 mmol/L. Deep hypothermic circulatory arrest (DHCA) duration, nadir hematocrit, and preoperative pulmonary vascular resistance were risk factors for lactate levels >7 mmol/L. Moreover, the intraoperative peak lactate level during PEA under DHCA was found to be a statistically significant predictor of major complications being associated with longer mechanical ventilation time ( r = 0.294; p = .003) and intensive care unit length of stay ( r = 0.327; p = .001). Conclusions Deep hypothermic circulatory arrest duration, nadir hematocrit, and preoperative pulmonary vascular resistance were associated with hyperlactatemia. Increased lactate levels were independent predictors of longer mechanical ventilation time, intensive care unit length of stay, and major complications.
Factors Affecting the Seeking of Orthodontic Treatment of Turkish Adults
Authors: Uslu Arzu Uçar , Buyuk Suleyman Kutalmıs 
Published: November 2023

Objective The aim of this study is to determine the factors that are effective in seeking orthodontic treatment of Turkish adult individuals and to evaluate the effects of demographic characteristics such as gender, age, marital status, and educational status of the participants on these factors comparatively. Methods A questionnaire-based study was applied to participants who want to receive orthodontic treatment. In the first part, the participants were asked about demographic information such as gender, age, marital status, occupation, and educational status. A total of 542 people (330 female and 212 male) were included in our study. The age of participants included in the study ranged between 18 and 45. Questions about oral hygiene, regular dental check-ups and the factors that affect in seeking orthodontic treatment were asked in the second part of the questionnaire. Results Young adults and females were found to have a higher rate of seeking orthodontic treatment. When the participants were asked what led them to orthodontic treatment, 72.1% of the participants stated that they thought it as a necessity such that they should receive orthodontic treatment. Material selection in orthodontic treatment shows statistical significance according to demographic characteristics ( p < 0.05). While 48% of the participants in the 18ȓ24 age group preferred metal orthodontic brackets, 37.9% of the participants in the 25–45 age group preferred clear aligner. Moreover, 40.4% of the married participants preferred clear aligner, while 45.1% of the single participants preferred metal orthodontic brackets. Conclusion The factors in seeking orthodontic treatment of individuals vary according to demographic characteristics and social environment of individuals.
In Vitro Evaluation of Vancomycin-Induced Toxicity in Human Primary Knee Chondrocytes
Authors: Hall Susan , Grayson Jane , Grant Gary , Vertullo Christopher , Anoopkumar-Dukie Shailendra 
Published: November 2023

Septic arthritis as a complication of orthopaedic joint surgery can have catastrophic outcomes for patients. To minimise infection risk associated with elective orthopaedics, topical vancomycin during surgery has become increasingly common. Evidence suggests that high concentrations of vancomycin, following direct application of the drug to the joint, are toxic towards various local cell types in the joint, including chondrocytes. However, the mechanism of this vancomycin tissue toxicity is yet to be determined. The aim of this study was to evaluate the toxicity of vancomycin on chondrocytes and the mechanisms of cell death involved. Human primary knee chondrocytes were exposed to vancomycin (1.25–10 mg/mL) for 24 h and their viability assessed using the resazurin reduction assay in vitro. Specific cell death mechanisms and their contributors, including reactive oxygen species (ROS) production and apoptosis, were measured. This study showed that high concentrations of vancomycin (5 and 10 mg/mL) were toxic towards human primary knee chondrocyte cells, while lower concentrations (1.25 and 2.5 mg/mL) were not. Cell death studies found that this occurred through an apoptotic pathway. This study provides additional support that vancomycin in high doses is toxic towards chondrocytes and preliminary evidence that this toxicity occurs via apoptotic cell death mechanisms.
A comparison of 3-D CT and 2-D plain radiograph measurements of the wrist in extra-articular malunited fractures of the distal radius
Authors: Smees Camiel J. , van Es Eline M. , Tuijthof Gabriëlle J. M. , Colaris Joost W. , de Graaff Feike , Vochteloo Anne J. H. 
Published: November 2023

Two-dimensional (2-D) plain radiographs may be insufficient for the evaluation of distal radial malunion, as it is a three-dimensional (3-D) deformity. This study introduced a 3-D measuring method that outputs radial inclination, ulnar variance, palmar tilt and axial rotation. To this end, a standardized and clearly defined coordinate system was constructed that allowed 3-D measurements closely resembling the conventional 2-D method in 35 patients. Mean differences between 3-D and 2-D measurements in affected wrists were 1.8° for radial inclination, 0.8 mm for ulnar variance and 3.7° for palmar tilt. In addition, inter- and intra-observer reproducibility of all 3-D and 2-D measurements were good or excellent (intraclass correlation coefficient >0.75), with 3-D reproducibility always better than 2-D. Axial rotation was present in all patients with a mean of 7.9° (SD 6.9). Although the differences between 2-D and 3-D measurements were small, 3-D evaluation enables the assessment of axial rotation and brings us closer to a routine 3-D evaluation of malunion. Level of evidence: III
Genome-wide identification and characteristic analysis of ETS gene family in blood clam Tegillarca granosa
Authors: Jin Hongyu , Zhang Weiwei , Liu Hongxing , Bao Yongbo 
Published: November 2023

Abstract
Background
ETS transcription factors, known as the E26 transformation-specific factors, assume a critical role in the regulation of various vital biological processes in animals, including cell differentiation, the cell cycle, and cell apoptosis. However, their characterization in mollusks is currently lacking.

Results
The current study focused on a comprehensive analysis of the ETS genes in blood clam Tegillarca granosa and other mollusk genomes. Our phylogenetic analysis revealed the absence of the SPI and ETV subfamilies in mollusks compared to humans. Additionally, several ETS genes in mollusks were found to lack the PNT domain, potentially resulting in a diminished ability of ETS proteins to bind target genes. Interestingly, the bivalve ETS1 genes exhibited significantly high expression levels during the multicellular proliferation stage and in gill tissues. Furthermore, qRT-PCR results showed that Tg-ETS-14 (ETS1) is upregulated in the high total hemocyte counts (THC) population of T. granosa, suggesting it plays a significant role in stimulating hemocyte proliferation.

Conclusion
Our study significantly contributes to the comprehension of the evolutionary aspects concerning the ETS gene family, while also providing valuable insights into its role in fostering hemocyte proliferation across mollusks.
EMS-induced mutagenesis in Choy sum (Brassica chinensis var. parachinensis) and selection for low light tolerance using abiotic stress indices
Authors: Deivanai Subramanian , Sng Benny Jian Rong , Van Vu Kien , Shibu Thankaraj Salammal Maria , Jang In-Cheol , Ramachandran Srinivasan 
Published: November 2023

Abstract
Background
Choy Sum (Brassica rapa ssp. chinensis var. parachinensis), grown in a controlled environment, is vulnerable to changes in indoor light quality and displays distinct photo-morphogenesis responses. The scarcity of Choy Sum germplasm for indoor cultivation necessitates the development of new cultivars. Hence, this study attempted to develop mutants through chemical mutagenesis and select low-light-tolerant mutants by using abiotic stress tolerance indices.

Results
A mutant population of Choy Sum created using 1.5% ethyl methane sulfonate (EMS) at 4 h was manually pollinated to obtain the M2 generation. 154 mutants with reduced hypocotyl length were initially isolated from 3600 M2 seedlings screened under low light (R: FR = 0.5). Five mutants that showed reduced plant height at mature stages were selected and screened directly for shade tolerance in the M3 generation. Principal component analysis based on phenotypic data distinguished the M3 mutants from the wild type. Abiotic stress tolerance indices such as relative stress index (RSI), stress tolerance index (STI), geometric mean productivity (GMP), yield stability index (YSI), and stress resistance index (SRI) showed significant (P < 0.05), and positive associations with leaf yield under shade. M3-12–2 was selected as a shade-tolerant mutant based on high values of STI, YSI, and SRI with low values for tolerance (TOL) and stress susceptibility index (SSI).

Conclusions
The results demonstrate that mutation breeding can be used to create dominant mutants in Choy Sum. Furthermore, we show that screening for low light and selection based on abiotic tolerance indices allowed the identification of mutants with high resilience under shade. This method should apply to developing new cultivars in other crop plants that can be suitable for controlled environments with stable yield performance.
CoreSNP: an efficient pipeline for core marker profile selection from genome-wide SNP datasets in crops
Authors: Dou Tingyu , Wang Chunchao , Ma Yanling , Chen Zhaoyan , Zhang Jing , Guo Ganggang 
Published: November 2023

Abstract
Background
DNA marker profiles play a crucial role in the identification and registration of germplasm, as well as in the distinctness, uniformity, and stability (DUS) testing of new plant variety protection. However, selecting minimal marker sets from large-scale SNP dataset can be challenging to distinguish a maximum number of samples. Results: Here, we developed the CoreSNP pipeline using a “divide and conquer” strategy and a “greedy” algorithm. The pipeline offers adjustable parameters to guarantee the distinction of each sample pair with at least two markers. Additionally, it allows datasets with missing loci as input. The pipeline was tested in barley, soybean, wheat, rice and maize. A few dozen of core SNPs were efficiently selected in different crops with SNP array, GBS, and WGS dataset, which can differentiate thousands of individual samples. The core SNPs were distributed across all chromosomes, exhibiting lower pairwise linkage disequilibrium (LD) and higher polymorphism information content (PIC) and minor allele frequencies (MAF). It was shown that both the genetic diversity of the population and the characteristics of the original dataset can significantly influence the number of core markers. In addition, the core SNPs capture a certain level of the original population structure.

Conclusions
CoreSNP is an efficiency way of core marker sets selection based on Genome-wide SNP datasets of crops. Combined with low-density SNP chip or genotyping technologies, it can be a cost-effective way to simplify and expedite the evaluation of genetic resources and differentiate different crop varieties. This tool is expected to have great application prospects in the rapid comparison of germplasm and intellectual property protection of new varieties.
Brain MRI and EEG overemployment in patients with vasovagal syncope: results from a tertiary syncope unit
Authors: Tajdini Masih , Khalaji Amirmohammad , Behnoush Amir Hossein , Tavolinejad Hamed , Jalali Arash , Sadeghian Saeed , Vasheghani-Farahani Ali , Yadangi Somayeh , Masoudkabir Farzad , Bozorgi Ali 
Published: November 2023

Abstract
Background
The diagnosis of vasovagal syncope (VVS) is mainly based on history-taking and physical examination. However, brain Magnetic Resonance Imaging (MRI) and Electroencephalogram (EEG) are commonly used in the diagnostic course of VVS, despite not being indicated in the guidelines. This study aims to find the possible associated factors with the administration of brain MRI and EEG in patients with VVS.

Methods
Patients with a diagnosis of VVS from 2017 to 2022 were included. Several demographic and syncope features were recorded. The association of these was assessed with undergoing MRI, EEG, and either MRI or EEG. Univariate and multivariable logistic regression models were also used to calculate odds ratios (OR) and 95% confidence intervals (CI).

Results
A total of 1882 patients with VVS were analyzed, among which 810 underwent MRI (43.04%), 985 underwent EEG (52.34%), and 1166 underwent MRI or EEG (61.96%). Head trauma (OR 1.38, 95% CI 1.06 to 1.80), previous neurologist visit (OR 6.28, 95% CI 4.24 to 9.64), and gaze disturbance during syncope (OR 1.75, 95% CI 1.13 to 2.78) were all positively associated to the performance of brain MRI/EEG. Similar results were found for urinary incontinence (OR 2.415, 95% CI 1.494 to 4.055), amnesia (OR 1.421, 95% CI 1.053 to 1.930), headache after syncope (OR 1.321, 95% CI 1.046 to 1.672), and tonic-clonic movements in head-up tilt table test (OR 1.501, 95% CI 1.087 to 2.093). However, male sex (OR 0.655, 95% CI 0.535 to 0.800) and chest pain before syncope (OR 0.628, 95% CI 0.459 to 0.860) had significant negative associations with performing brain MRI/EEG.

Conclusion
Based on our findings, performing MRI or EEG was common among VVS patients while it is not indicated in the majority of cases. This should be taken into consideration to prevent inappropriate MRI/EEG when there is a typical history compatible with VVS.
Pepsinogen ratio and brachial-ankle pulse wave velocity: a cross-sectional study on their interrelationship in atherosclerosis
Authors: Li Yuexi , Liu Xiaoqin , Luo Yuhan , Wang Qiaoli 
Published: November 2023

Abstract
Background
Existing research has established the pepsinogen ratio (PGR) as a complex biomarker, not only as an independent predictor for various gastrointestinal diseases but also in its association with atherosclerotic cardiovascular diseases. However, the precise mechanism linking changes in PGR to cardiovascular pathologies remains unclear. The objective of this study is to quantitatively elucidate the association between PGR and brachial-ankle pulse wave velocity (baPWV) as an indicator of atherosclerotic progression.

Methods
We conducted a cross-sectional study that analyzed clinical data from 465 patients who underwent health screenings. One-way Analysis of Variance (ANOVA) identified potential risk factors affecting baPWV. Multiple logistic regression was employed to evaluate if PGR serves as an independent risk factor for elevated baPWV after accounting for these variables. Generalized additive models and smoothed curve fitting were utilized to investigate the possibility of a nonlinear association between PGR and baPWV. When such nonlinearity was found, threshold effect analysis pinpointed the inflection point in this relationship, followed by segmented correlation analyses.

Results
PGR negatively correlated with both right baPWV (RbaPWV) and left baPWV (LbaPWV) after adjusting for confounders. Smoothed curve analyses revealed nonlinear relationships, with inflection points at 22.5 for RbaPWV and 22.3 for LbaPWV. For PGR values below 22.5, a significant negative correlation with RbaPWV was observed (β = − 6.3 cm/s, P < 0.001). Conversely, for PGR values above 22.5, no significant linear relationship was found (P = 0.141). Similarly, when PGR was below 22.3, a strong negative correlation with LbaPWV was detected (β = − 7.0 cm/s, P < 0.001), but such correlation was absent for higher PGR levels (P = 0.273).

Conclusion
The study reveals that PGR is associated with RbaPWV and LbaPWV in a nonlinear manner. Specifically, lower levels of PGR were linearly and inversely correlated with baPWV, but this relationship became nonlinear at higher PGR levels. These findings suggest that modulating PGR levels may offer a therapeutic strategy for managing atherosclerosis.
Emphysematous changes as red flag signs preceding rapidly progressive infectious aortic disease: two case reports
Authors: Higuchi Yusuke , Nomura Tetsuya , Yoshida Shiori , Kitamura Michitaka , Ono Kenshi , Shoji Keisuke , Wada Naotoshi , Keira Natsuya , Tatsumi Tetsuya 
Published: November 2023

Abstract
Background
Infectious aortic disease is a rare and fatal disease, that requires the appropriate intervention. An accurate diagnosis should be promptly established. However, this is difficult because the clinical manifestations of this disease vary and are non-specific.

Case presentation
(CASE 1) An 87-year-old male, presenting with generalized malaise and weight loss, was admitted for further examination. A chest computed tomography (CT) showed mediastinal emphysema. Empirical intravenous antibiotics were administered to address the non-specific infectious findings in the laboratory data. The treatment was effective, and the patient fully recovered. However, he was in shock due to aortic rupture and marked pseudo aneurysmal formation around the aortic arch day 25 of hospitalization. An emergency total aortic arch replacement was performed, and the patient was discharged. (CASE 2) An 82-year-old male who had undergone Y-graft replacement in the abdominal aorta 15 years previously was admitted due to general malaise and anorexia. Abdominal CT revealed emphysematous changes adjacent to the abdominal aorta. The patient responded favorably to empirical treatment with intravenous antibiotics and was discharged 19 days after admission. Four days after discharge, the patient went into cardiac arrest after an episode of hematemesis. Abdominal CT revealed an enlarged stomach and duodenum, filled with massive high-density contents proximal to the abdominal aorta. He died of hemorrhagic shock despite cardiopulmonary resuscitation.

Conclusions
Although emphysematous changes are rare, they are red flag signs during the early stage of infectious aortic disease. Thus, physicians should remain vigilant for this kind of critical sign.