bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of impurity profiling via method development and force degradation in hydrocortisone butyrate at low concentration
Authors: Gunjal Harshwardhan G. , Byahatti Vivek V. 
Published: November 2023

Abstract
Background
Since long, triamcinolone and fluocinolone, fluorinated derivatives, have been used for treating various types of dermatitis. Rosacea and perioral dermatitis are the most common side effects observed with prolonged use of fluorinated derivatives. Recent studies have shown that these adverse effects are more severe at low doses of fluorinated derivatives compared to low doses of non-fluorinated derivatives. Therefore, this study focused on impurity profiling through force degradation studies of hydrocortisone butyrate in a 0.1% lotion.

Results
A precise and robust method with low concentration was established for the estimation of hydrocortisone butyrate in bulk and formulations was done using the RP-HPLC technique. The mobile phase consisted of a combination of acetonitrile and purified water (30:70% v/v) and acetonitrile and purified water (95:5% v/v), with detection at a wavelength of 254 nm and a total run time of 20 min. The method demonstrated linearity and accuracy within the concentration range of 0.1–250 µg/mL and 50–125 µg/mL, respectively, with an r2 value of 0.999. Stress stability studies were conducted on hydrocortisone butyrate, revealing 11% degradation in alkaline conditions and 18% degradation in photolytic conditions.

Conclusion
The established method can be commercially used as it exhibits excellent linearity. Impurities were identified by injecting the reference standard, and their retentions were confirmed. The identified impurities included hydrocortisone, hydrocortisone-21-butyrate, hydrocortisone 3-methyl enol ether 17-butyrate, and hydrocortisone 17, 21-methylorthobutyrate, with retention times of 2.89, 9.14, 13.70, and 16.25 min, respectively. This precise method can be utilized in commercial applications for the accurate identification of hydrocortisone butyrate at low concentrations.
Clinical Validation of Human Papilloma Virus Circulating Tumor DNA for Early Detection of Residual Disease After Chemoradiation in Cervical Cancer
Authors: Han Kathy , Zou Jinfeng , Zhao Zhen , Baskurt Zeynep , Zheng Yangqiao , Barnes Elizabeth , Croke Jennifer , Ferguson Sarah E. , Fyles Anthony , Gien Lilian , Gladwish Adam , Lecavalier-Barsoum Magali , Lheureux Stephanie , Lukovic Jelena , Mackay Helen , Marchand Eve-Lyne , Metser Ur , Milosevic Michael , Taggar Amandeep S. , Bratman Scott V. , Leung Eric 
Published: November 2023

PURPOSE Most cervical cancers are caused by human papilloma virus (HPV), and HPV circulating tumor DNA (ctDNA) may identify patients at highest risk of relapse. Our pilot study using digital polymerase chain reaction (dPCR) showed that detectable HPV ctDNA at the end of chemoradiation (CRT) is associated with inferior progression-free survival (PFS) and that a next-generation sequencing approach (HPV-seq) may outperform dPCR. We aimed to prospectively validate HPV ctDNA as a tool for early detection of residual disease. METHODS This prospective, multicenter validation study accrued patients with stage IB-IVA cervical cancer treated with CRT between 2017 and 2022. Participants underwent phlebotomy at baseline, end of CRT, 4-6 weeks post-CRT, and 3 months post-CRT for HPV ctDNA levels. Plasma HPV genotype–specific DNA levels were quantified using both dPCR and HPV-seq. The primary end point was 2-year PFS. RESULTS With a median follow-up of 2.2 (range, 0.5-5.5) years, there were 24 PFS events among the 70 patients with HPV+ cervical cancer. Patients with detectable HPV ctDNA on dPCR at the end of CRT, 4-6 weeks post-CRT, and 3 months post-CRT had significantly worse 2-year PFS compared with those with undetectable HPV ctDNA (77% v 51%, P = .03; 82% v 15%, P < .001; and 82% v 24%, P < .001, respectively); the median lead time to recurrence was 5.9 months. HPV-seq showed similar results as dPCR. On multivariable analyses, detectable HPV ctDNA on dPCR and HPV-seq remained independently associated with inferior PFS. CONCLUSION Persistent HPV ctDNA after CRT is independently associated with inferior PFS. HPV ctDNA testing can identify, as early as at the end of CRT, patients at high risk of recurrence for future treatment intensification trials.
Association Between Homologous Recombination Repair Biomarkers and Survival in Patients With Solid Tumors
Authors: Shao Changxia , Ren Yixin , Zhou Heng , Chen Cai , Dettman Elisha J. , Lee Liam C. , Cristescu Razvan , Gozman Alexander , Jin Fan , Zhou Wei 
Published: November 2023

PURPOSE Mutations in BRCA1 and/or BRCA2 (BRCAm), other homologous recombination repair genes (HRRm), and homologous recombination deficiency (HRD) lead to an accumulation of genomic alterations that can drive tumorigenesis. The prognostic impact of these HRR pathway defects on overall survival (OS) in patients not receiving poly (ADP-ribose) polymerase inhibitors (PARPi) or immunotherapy is unclear. We evaluated the association of HRR biomarkers with OS in patients with advanced solid tumors receiving therapy excluding PARPi and immunotherapy. METHODS Deidentified data were collected through December 31, 2020, from a real-world clinicogenomic database (CGDB) with data originating from approximately 280 cancer clinics in the United States. Patients age 18 years and older with an advanced/metastatic diagnosis between 2018 and 2019 for 1 of 15 solid tumors and available data in the CGDB were included. The primary analysis evaluated the association between HRR pathway biomarkers and OS, using start of second-line therapy as the index date (to reduce immortal time bias). RESULTS A total of 9,457 patients had available data for BRCA/HRR and 5,792 for HRD status; 4,890 (51.7%) were women and mean (SD) age was 65.9 (11.5) years. For the primary analysis, adjusted hazard ratios for OS were BRCAm (n = 156) versus BRCA wild-type (wt; n = 3,131; 0.83 [95% CI, 0.60 to 1.17]); for HRRm (n = 467) versus HRRwt (n = 282; 0.95 [95% CI, 0.79 to 1.14]); and for HRD-positive (n = 447) versus -negative (n = 1,687; 1.22 [95% CI, 1.02 to 1.47]). Results were similar using start of first-line and start of third-line therapy as index dates. CONCLUSION This large, real-world study found no association between OS and either BRCA or HRR status but identified a possible linkage between HRD positivity and shorter median OS in patients with advanced solid tumors who did not receive PARPi or immunotherapy.
Neurodevelopmental Programming of Adiposity: Contributions to Obesity Risk
Authors: Skowronski Alicja A , Leibel Rudolph L , LeDuc Charles A 
Published: November 2023

Abstract
This review analyzes the published evidence regarding maternal factors that influence the developmental programming of long-term adiposity in humans and animals via the central nervous system (CNS). We describe the physiological outcomes of perinatal underfeeding and overfeeding and explore potential mechanisms that may mediate the impact of such exposures on the development of feeding circuits within the CNS—including the influences of metabolic hormones and epigenetic changes. The perinatal environment, reflective of maternal nutritional status, contributes to the programming of offspring adiposity. The in utero and early postnatal periods represent critically sensitive developmental windows during which the hormonal and metabolic milieu affects the maturation of the hypothalamus. Maternal hyperglycemia is associated with increased transfer of glucose to the fetus driving fetal hyperinsulinemia. Elevated fetal insulin causes increased adiposity and consequently higher fetal circulating leptin concentration. Mechanistic studies in animal models indicate important roles of leptin and insulin in central and peripheral programming of adiposity, and suggest that optimal concentrations of these hormones are critical during early life. Additionally, the environmental milieu during development may be conveyed to progeny through epigenetic marks and these can potentially be vertically transmitted to subsequent generations. Thus, nutritional and metabolic/endocrine signals during perinatal development can have lifelong (and possibly multigenerational) impacts on offspring body weight regulation.
Integrin-based adhesions promote cell–cell junction and cytoskeletal remodelling to drive embryonic wound healing
Authors: Ly Michelle , Schimmer Clara , Hawkins Raymond , E. Rothenberg Katheryn , Fernandez-Gonzalez Rodrigo 
Published: November 2023

ABSTRACT
Embryos repair wounds rapidly, with no inflammation or scarring. Embryonic wound healing is driven by the collective movement of the cells around the lesion. The cells adjacent to the wound polarize the cytoskeletal protein actin and the molecular motor non-muscle myosin II, which accumulate at the wound edge forming a supracellular cable around the wound. Adherens junction proteins, including E-cadherin, are internalized from the wound edge and localize to former tricellular junctions at the wound margin, in a process necessary for cytoskeletal polarity. We found that the cells adjacent to wounds in the Drosophila embryonic epidermis polarized Talin, a core component of cell–extracellular matrix (ECM) adhesions, which preferentially accumulated at the wound edge. Integrin knockdown and inhibition of integrin binding delayed wound closure and reduced actin polarization and dynamics around the wound. Additionally, disrupting integrins caused a defect in E-cadherin reinforcement at tricellular junctions along the wound edge, suggesting crosstalk between integrin-based and cadherin-based adhesions. Our results show that cell–ECM adhesion contributes to embryonic wound repair and reveal an interplay between cell–cell and cell–ECM adhesion in the collective cell movements that drive rapid wound healing.
Comparison of the methods of the correction of the venous thromboembolism in bariatric surgery using the low-frequency thromboelastography: our experience
Authors: Tarabrin O. , Sukhonos R. , Suslov O. , Kirpichnikova K. , Bugaenko E. 
Published: November 2023

Introduction. In the world of obesity and its complications annually dies about 2.8 million people. Obesity is an independent factor of the risk of venous thromboembolism (VTE), as it interferes with the internal and external pathways of coagulation, as well as in anticoagulant mechanisms, which leads to a hypercoagulation state. Reducing excess body weight in patients with morbid obesity by bariatric surgery is becoming increasingly popular, as barium therapy is an effective means of treating obesity and related concomitant diseases. Advantages of bariatric surgery are undeniable, as well as risks. Despite many blood coagulation studies, cases of thromboembolism in such patients are becoming more frequent, especially during surgical interventions and in the postoperative period, given that the operation is a trigger factor in the development of thromboembolism.
Materials and methods. A hemostasis system was studied in 63 patients with BMI> 35 kg / m2. All patients were divided into 3 groups. 1 group (n=20) - patients who received combined therapy: Enoxaparin sodium 0.1% 0.2 ml / kg 2 times a day every 12 hours and Pentoxifylline 100 mg 2 times a day every 12 hours; The 2nd group (n= 17) - patients treated with: Enoxaparin sodium 0.1% 0.2 ml / kg 2 times daily for every 12 hours; Group 3 (control): 26 people with obesity, BMI> 35, not subject to bariatric surgery. The study of the hemostasis system was carried out using low-frequency piezoelectric thromboelastography (LPTEG) immediately after hospitalization and on 1,3,5 days after bariatry. The following blood coagulation constants were checked: Contact Coagulation Intensity; intensity of coagulation drive; maximum bunch density; Fibrinolytic activity is the index of retraction and lysis of the clot.
Results. In patients of group 1 (anticoagulants + antiplatelet agents) the risk of thrombotic complications is lower, since this therapy has reduced the value at all stages of hemocoagulation to the reference values; Patients in group 2 (anticoagulants), despite the normalization of the coagulation unit, had an increase in the values of aggregation and fibrinolysis, relative to the norm, which means the risk of thrombotic complications remains high.
Conclusions. The instrumental method of NPTEG allowed to adequately estimate the system of hemocoagulation in dynamics in patients with morbid obesity, with BMI ≥ 35 kg / m2; This category of patients has shown a high risk of VTE; It has been proved that for an adequate prevention of VTE in obese patients undergoing bariatric surgery, the combination of anticoagulants and antiplatelet agents is more effective than monotherapy with anticoagulants.
Behavioral Microfoundations of New Practice Adoption: The Effects of Rewards, Training and Population Dynamics
Authors: Feylessoufi Antoine , Kavadias Stylianos , Ralph Daniel 
Published: November 2023

Organizations face challenges when trying to effectively introduce new operational practices that substitute for existing ones. We study how the dynamics due to social comparisons between employees give rise to individual strategic considerations and eventually shape the organizational adoption outcome. We develop an evolutionary game theory model that accounts for these microlevel individual adoption decisions and their impact on macrolevel population adoption equilibria. Social comparisons invoke dynamics that expand the possible outcomes beyond the traditional nonadoption versus full-adoption dichotomy. Specifically, ahead-seeking social comparisons drive the long-term coexistence of practices because employees seek to differentiate their choices from those of others. Meanwhile, behind-averse comparisons create a bandwagon effect that determines adoption depending on the initial fraction of adopters—that is, employees who are trained upfront. These dynamics are robust to various settings: different conceptualizations of social comparisons, each employee responding to more than one kind of social comparison, and nonhomogeneous social comparisons across employees. Moreover, they are material to organizations that seek to maximize their profit when introducing a new practice, by setting the levels of upfront training and adoption rewards. Our results call for senior managers to account for such behavioral traits when managing the introduction of new practices. Profitable adoption critically relies upon matching rewards and training to the type of social comparison. This paper was accepted by Sridhar Tayur, entrepreneurship and innovation. Supplemental Material: The online appendix is available at https://doi.org/10.1287/mnsc.2022.00305 .
DIREITO PROCESSUAL ROMANO
Authors: Gonçalves de Castro Mendes Aluisio , Paes de Castro Mendes Carolina 
Published: November 2023

O Código de Processo Civil de 2015 e o contemporâneo debate sobre o Poder Judiciário refletem a preocupação da sociedade para com a sua Justiça. Hoje, no meio jurídico, buscam-se soluções para os problemas relacionados com a prestação jurisdicional. Todavia, para se conhecer o presente e projetar o futuro, é fundamental o estudo do passado, restaurando-se as experiências bem-sucedidas e apropriadas à época atual e evitando-se os erros, além da compreensão do Direito dentro de um contexto maior, seja no tempo, no espaço e nas suas relações com a superestrutura. Nessa perspectiva, o presente estudo do se volta ao passado objetivando demonstrar a a suma importância do estudo do direito processual romano para o aprimoramento do direito processual. Partindo desse pressuposto, através de referências bibliográficas e documentais, o trabalho apresenta uma análise crítica e dinâmica do direito processual romano, de modo crítico e dinâmico, para contribuir com as discussões modernas sobre a justiça e a prestação jurisdicional. O artigo promove uma recapitulação do processo civil romano, abordando os períodos pré-classico, clássico e pós-classico e os três sistemas – legis actiones, formulae e cognitio extra ordinem – respectivamente situados nos períodos mencionados. Pretende, ainda, apresentar observações sobre o processo civil e a justiça na atualidade, em confrontação com o direito processual romano, especialmente o que se encontra situado nos períodos pré-clássico e clássico. São destacadas características como a oralidade, recorribilidade, a possibilidade do non liquet, entre outros. O estudo direito romano permite várias outras visões ou enfoques do direito, no seu estado, talvez, mais puro e forte, fazendo com que, através de confrontações e estudos, se possa ver melhor ou, pelo menos, com outros olhos, a nossa realidade, impedindo a acomodação e estimulando a elaboração de propostas e ideias, das quais as proposições acima são, tão-somente, um esboço singelo, como uma gota no grande oceano de possibilidades.
Common adolescent mental health disorders seen in Family Medicine Clinics in Ghana and Nigeria
Authors: Kumbet Sonny John , Oseni Tijani Idris Ahmad , Mensah-Bonsu Magdalene , Damagum Fatima Mohammed , Opare-Lokko Edwina Beryl Addo , Namisango Eve , Olawumi AbdulGafar Lekan , Ephraim Onyenwe Chibuike , Aweh Benjamin 
Published: November 2023

BackgroundMental health disorders among adolescents is on the rise globally. Patients seldom present to mental health physicians, for fear of stigmatization, and due to the dearth of mental health physicians. They are mostly picked during consultations with Family Physicians. This study seeks to identify the common mental health disorders seen by family Physicians in Family Medicine Clinics in Nigeria and Ghana.MethodsA descriptive cross-sectional study involving 302 Physicians practicing in Family Medicine Clinics in Nigeria and Ghana, who were randomly selected for the study. Data were collected using self-administered semi-structured questionnaire, and were entered into excel spreadsheet before analysing with IBM-SPSS version 22. Descriptive statistics using frequencies and percentages was used to describe variables.ResultsOf the 302 Physicians recruited for the study, only 233 completed the study, in which 168 (72.1%) practiced in Nigeria and 65 (27.9%) in Ghana. They were mostly in urban communities (77.3%) and tertiary health facilities (65.2%). Over 90% of Family Medicine practitioners attended to adolescents with mental health issues with over 70% of them seeing at least 2 adolescents with mental health issues every year. The burden of mental health disorder was 16% and the common mental health disorders seen were depression (59.2%), Bipolar Affective Disorder (55.8%), Epilepsy (51.9%) and Substance Abuse Disorder (44.2%).ConclusionFamily Physicians in Nigeria and Ghana attend to a good number of adolescents with mental health disorders in their clinics. There is the need for Family Physicians to have specialized training and retraining to be able to recognize and treat adolescent mental health disorders. This will help to reduce stigmatization and improve the management of the disease thus, reducing the burden.
Assessment of COVID-19 vaccine acceptance and its associated factors in Debre Berhan City, Ethiopia, 2022
Authors: Wubshet Eyuel , Dagnaw Abinet , Gebrelibanos Helen , Tefera Mitiku 
Published: November 2023

Background
The COVID-19 pandemic has spread over the world. The ability to achieve sufficient immunization coverage to end the global pandemic depends on the acceptance of the COVID-19 vaccine, but it has faced a major challenge around the world. In low-income and developing countries, 22.7% of the population has received at least one dose of the Covid-19 vaccine, which means that a large percentage of the population are unvaccinated, even though they have access to the Covid-19 vaccine so many countries do not accept the vaccine. The aim of this study was to assess COVID-19 vaccine acceptance and its associated factors in Debre Berhan City, Ethiopia, 2022.

Methods
A mixed-methods approach comprising both qualitative interviews and a quantitative survey was used among participants in Debre Berhan City. A multi-stage sampling technique was used to recruit the study participants. An in-depth interview was used for the qualitative data. Data was collected by a face-to-face interview questionnaire from June 08 to July 08, 2022. The collected data was entered using Epi Data version 4.6 and analyzed using SPSS version 25. Variables with a p-value less than 0.25 at Bivariable logistic regression analysis were entered into multivariable logistic regression analysis. Logistics regression was employed, and a p-value <0.05 was considered statistically significant.

Result
A total of 765 participants were included in the study, with a response rate of 97.08%. More than half (52.9%) of the respondents had the willingness to accept the COVID-19 vaccine. Participants who had Contact with COVID-19 patient (AOR = 3.98; 95% CI: (1.30–12.14), having good knowledge of COVID-19 vaccine (AOR = 4.63; 95% CI: (1.84–11.63), and positive attitude toward the COVID-19 vaccine (AOR = 3.41; 95% CI: (1.34–8.69) were statistically significantly associated variables with COVID-19 vaccine acceptance.

Conclusion and recommendation
The present study revealed that the acceptance COVID-19 vaccine was 52.9, and a significant proportion of participants were hesitant to receive the vaccine and refused to get vaccinated. Significantly associated Variables for COVID-19 vaccine acceptance were Contact with COVID-19 patient, having good knowledge of the COVID-19 vaccine, and having a positive attitude towards the COVID-19 vaccine. Various stakeholders to apprise the public about the cause of the disease and the scientific development of vaccine in order to enhance acceptance of the vaccine.
Measuring the relationship between body mass index and depression among Saudi adult population: A nationwide cross-sectional study
Authors: Nour Mohamed O. , Hafiz Tamara Abdulrahman , Alharbi Khulud K. 
Published: November 2023

Background
The prevalence of obesity and depression shows an accelerating trend with increased risk of morbidity and disability. The exact underlying relationship between them is unclear. We aimed to evaluate the prevalence of body mass index (BMI) and depression and their associations in a large sample of Saudi adults.

Methods
We administered a nationwide cross-sectional web-based survey using a snowball sampling method among Saudi adults aged 18–60 years. We used a validated Arabic version of Beck’s Depression Inventory-II (BDI-II) for depression assessment. We classified BMI into underweight, normal weight, overweight, and obesity. We used logistic regression analysis to determine the factors associated with depression.

Results
Among 4,683 Saudi adults, different grades of depression were present in 43.3%, most (25.2%) with a mild condition. Overweight and obesity were present in 26.4% and 21%, respectively. We found a positive association between BMI and BDI-II score (ρ = 0.14, p = 0.006). BMI was significantly higher among those who were older, males, married, living in the Eastern region of Saudi Arabia, educated at a pre-university level, employed, at high family-income levels, smokers, and people with chronic diseases. Depression score was significantly higher among married, non-employees, non-smokers, people with chronic diseases, and those with BMI ≥ 25 kg/m2. Non-smoking, presence of chronic diseases, and being overweight or obese were significantly associated with depression.

Conclusions
Saudi adults were suffering from different grades of depression, overweight, and obesity. A positive association between BMI and BDI-II score was observed. Depression score did not differ by age, sex, geographical region, educational level, or family income. Non-smoking, presence of chronic diseases, and being overweight or obese were significantly associated with depression. Further longitudinal research is required to understand the factors underpinning causal relationships between BMI and depression, the subgroups’ variation, and mediating strategies.