bluuebunny/crossref_metadata_2025_split huggingface dataset:
O uso dos objetos digitais de aprendizagem “Massa Molar” e “Concentrações” no ensino médio durante o ensino remoto
Authors: Ferraz Rodrigo da Silva , Carvalho José Wilson Pires , Negreiros Cláudia Landin 
Published: October 2023

As Tecnologias Digitais da Informação e Comunicação (TDICs) tem feito parte do nosso cotidiano, seja na vida social, no trabalho e no ensino. Considerando esse contexto da TDICs, o presente estudo teve, como objetivo, compreender as contribuições dos Objetos Digitais de Aprendizagem (ODA) “Massa Molar” e “Concentrações” nos processos de ensino de conteúdos de Química no Ensino Médio. A pesquisa foi de natureza qualitativa, com uso de questionário, roda de conversa e observação. Os participantes foram alunos do Ensino Médio de uma Escola Estadual do interior do estado de Mato Grosso. Os ODA utilizados foram o “Massa Molar” e “Concentrações” do site Simulações PhET, em encontros virtuais. A análise de dados foi feita pelo método indutivo. Os resultados mostraram que o uso dos ODA pode contribuir para os processos de ensino dos conteúdos de Química porque permitem a visualização de modelos que constituem os conceitos químicos e, com isso, podem acurar a percepção dos alunos quanto aos cálculos e fórmulas químicas estudadas. As atividades realizadas com os ODA contribuíram também para tornar as aulas mais dinâmicas e atrativas chamando a atenção dos mesmos. Os ODA se mostraram recursos aliados aos processos de Ensino da Química.
Risk Factors for Abnormal Hemoglobin Levels in Online Motorcycle Taxi Drivers in the Tanjung Mas Industrial Area, Semarang City
Authors: Setyowatiningsih Lilik , Wikandari Ririh Jatmi 
Published: October 2023

Air pollution can affect health conditions, CO and Pb are one of the compositions of vehicle exhaust gases that are dangerous. Online motorcycle taxi drivers have a high risk of exposure to pollutants. This research aims to determine the risk factors that affect hemoglobin levels in online motorcycle taxi drivers in the Tanjung Emas Area of Semarang City. The method used observational analytics with a cross-sectional approach. The total population in this study was 55 respondents and the number of respondents was determined using total sampling. Based on the results of examining hemoglobin levels using a hematology analyzer, of 55 respondents, 47 respondents had normal hemoglobin levels and 8 respondents had abnormal hemoglobin levels. The results of the Chi square test on smoking status have a significant relationship (p=0.04) to the blood hemoglobin level of online motorcycle taxis in the Tanjung Emas Area of Semarang city while in the status of Education, length of work, and knowledge there is no significant relationship (0.05) to the hemoglobin level of online motorcycle taxi drivers in the Tanjung Emas Area of Semarang City. The conclusion of this study is that there is a significant relationship between smoking status and hemoglobin levels of online motorcycle taxi drivers in the Tanjung Emas Area of Semarang City and there is no significant relationship between education factors, length of work time, and knowledge of hemoglobin levels of online motorcycle taxis in the Tanjung Emas Area of Semarang City.
Analysis of trends and necessity of extemporal compounding of medicinal forms in pharmacies during wartime and the COVID-19 pandemic in Ukraine
Authors: Заліська О. М. , Заболотня З. О. , Семенов О. М. , Максимович Н. М. , Калинюк Т. Г. , Барчук О. З. 
Published: October 2023

The need to saving of the pharmacies are preparing extemporaneous dosage forms (DF) is substantiated, and their effectiveness in the conditions of the coronavirus pandemic, martial law is shown.
The purpose of work was to conduct an analysis of the dynamics of the pharmacy number that manufacture EDF on the example of the Lviv region, the city of Lviv during 30 years, in order to determine trends. It was to study the list of DFs that were extemporaneously compounded. The list of DFs was analyzed, with an emphasis on the list of disinfectant solutions for sanitizing hands during the COVID-19 pandemic and wartime. The analysis objects were documents, order books of pharmacies with production departments during 30 years of activity.
The list of pharmaceuticals from 14 pharmacies that have valid licenses and actually carry out extemporaneous production in the city of Lviv, the Lviv region was studied. Research methods: historical, bibliographic, documentary, content analysis, systematization and generalization.
We have selected a set of 11 dosage forms, which were most often compounded during the coronavirus pandemic and martial law during 2020-2023, these prescriptions can be recommended for implementation in the work of compounding departments of private pharmacies. It was found that most industrial disinfectant solutions contain 60% isopropyl alcohol or 70%‒75% ethyl alcohol, so these solutions do not meet WHO recommendations for disinfection during a pandemic COVID-19.
It was established that the costs of disinfectant solutions per one litre were higher by 44.4% compared to one litre of extemporaneous production disinfectants, which has significant economic benefits for the rational use of budget funds.
It was substantiated that the urgent task of harmonizing with the legislation of the European Union regarding the work of pharmacies that have extemporaneous production requires the introduction of changes to the Resolution of the CMU dated 30.11.2016 N 929 on Licensing conditions for conducting business activities in the production of medicinal products, wholesale and retail trade medicinal products, on the mandatory extemporaneous production of medicinal forms in each pharmacy, regardless of the form of ownership.
It was established that 71.4% of extemporaneous pharmacies are communally owned, when chain pharmacies do not perform such a function at all, therefore it is necessary to harmonize with EU requirements for the presence of compounding departments in each pharmacy with good conditions and personnel for production.
The need for a hospital pharmacy with appropriate staff in each hospital for the preparation of sterile and non-sterile medical forms in Ukraine, and to ensure the practice of students and the internship of pharmacists is substantiated.
Pharmacoeconomic analysis of the use of combinations of complex electrolyte compounds with citicoline in the treatment of patients with acute ischemic stroke in Ukraine
Authors: Костюк І. А. , Міщенко Т. С. , Шалабін Є. Є. 
Published: October 2023

Ischemic stroke is a clinical syndrome of rapid development of signs of focal or global loss of brain function that lasts 24 hours or more or leads to death without other (nonvascular) causes. Cerebral stroke is one of the most severe forms of cerebrovascular disease.
A significant place in stroke pharmacotherapy is occupied by prescription drugs that increase the preservation of nervous tissue. Citicoline is an essential mediator in phosphatidylcholine biosynthesis, a major component of biological membranes.
The study aimed to analyze the cost-effectiveness and budget impact on using of complex electrolyt compounds with citicoline combinations in treatment of patients with acute ischemic stroke in Ukraine.
The cost analysis was based on a decision tree model developed for treatment within 28 days after an ischemic stroke, including the likelihood that a patient will need to undergo rehabilitation care in an out-patient or in-patient treatment.
The cost of direct expenses was determined based on the results of the course of Neurocytin® in combination with standard therapy, compared to the use of standard therapy alone, which, according to the results of the modelled analysis, is 30 407.10 UAH and 28 171.43 UAH per 1 person, respectively. Due to performing a pharmacoeconomic analysis using the cost-effectiveness method, the cost-utility indicator was calculated, which for treatment with Neurocytin® is 47 142.79 UAH, and for standard therapy – 46 031.74 UAH. The calculated ICUR value is 68 996.36 UAH, which indicates that the technology of Neurocytin® in combination with standard therapy is more effective and more costly. The ratio of the potential threshold of «willingness to pay» and the ICUR value visually demonstrates that in terms of cost-effectiveness, the technology of Neurocytin® in combination with standard therapy is within the threshold of willingness to pay from the perspective of the state budget.
Based on the budget impact analysis results, it was found that the cost of Scenario 3 (an annual 20% increase in the number of patients prescribed Neurocytin® in combination with standard therapy) will amount to 17.2 billion UAH.
Intensi Berwirausaha Siswa SMK Negeri di Kota Tangerang Selatan
Authors: Lestari Ambar Widya , Sulistiyani Sulistiyani , Anjani Septi Rostika 
Published: October 2023

Tujuan penelitian ini adalah untuk mengetahui apakah ada pengaruh gender, latar belakang orang tua, latar belakang pendidikan, dan efikasi diri terhadap intensi berwirausaha siswa SMK di Kota Tangerang Selatan. Metode yang digunakan adalah kuantitatif dengan pendekatan survey dengan teknik random sampling. Sampel penelitian ini berjumlah 379 responden. Teknik pengumpulan data ini menggunakan observasi, wawancara serta angket atau kuesioner. Metode pengujian data menggunakan uji validitas dan uji reliabilitas, metode analisis data menggunakan pengujian regresi dan pengujian hipotesis. Hasil penelitian variabel gender dan latar belakang pendidikan tidak memiliki pengaruh terhadap intensi berwirausaha siswa SMK Negeri di Kota Tangerang Selatan, adapun efikasi diri berpengaruh terhadap Intensi Berwirausaha Siswa SMK Negeri di Kota Tangerang Selatan. Secara simultan variabel efikasi diri dan latar belakang orang tua memiliki pengaruh terhadap intensi berwirausaha siswa SMK Negeri di Kota Tangerang Selatan terlihat dari nilai probabilitasnya (sig.) ternyata 0,000 < 0,05.
Pengaruh Work Environment, Work-Life Balance terhadap Employee Performance pada Karyawan di PT. Nusa Toyotetsu Corporation
Authors: Firanti Sherly Nira , Berliyanti Dita Oki 
Published: October 2023

Penelitian ini bertujuan untuk menganalisis pengaruh Work Environment dan Work-Life Balance terhadap Employee Engagement pada karyawan di PT Nusa Toyotetsu Corp. Pengambilan sampel yang digunakan dalam penelitian ini adalah purposive sampling kepada 55 karyawan di PT Nusa Toyotetsu Corp. dengan menyebarkan kuesioner secara online. Metode analisis data yang digunakan merupakan metode regresi berganda dengan SPSS versi 25. Dengan menggunakan metode pengumpulan data melalui kuesioner, data yang diperoleh dari penelitian dianalisis dengan metode analisis kuantitatif yaitu persamaan regresi berganda. Hasil penelitian menunjukkan bahwa Work Environment berpengaruh terhadap Employee Performance. Work-Life Balance berpengaruh terhadap Employee Performance. Selanjutnya, perusahaan perlu meningkatkan keseimbangan kerja karyawan, memperbaiki kondisi lingkungan dan memperhatikan stress karyawan, yang kesemuanya dilakukan untuk meningkatkan kinerja karyawan.
Patterns of Language Maintenance and Use in the Family Environment in Negeri Wakasihu, Maluku
Authors: Erniati Erniati , Wijaya Dendi 
Published: October 2023

This research investigates the patterns of language use and maintenance of communities in Wakasihu State, Central Maluku, and highlights various factors that influence language maintenance. This research seeks to reveal the complexity of maintaining the use of regional languages in Wakasihu State by looking at social variables such as age, gender, education and employment on language maintenance patterns. By using a quantitative descriptive approach enriched with mixed methods, this study combines the depth of qualitative insight with the breadth of quantitative data. Qualitative techniques, including observation, note-taking and interviews, provide a complex picture of the linguistic landscape of the Wakasihu community. To complement this, quantitative data was collected through a random questionnaire distributed to 50 communities. This questionnaire investigated the use of regional languages, their users, and how they are maintained. The findings of this study explain the dynamics of the use of interesting language in Wakasihu Country. Age is an important factor, the older generation shows a higher level of language retention. In contrast, the younger generation shows a lower level of retention of their local language. This generational shift signifies a change in the linguistic landscape in society. This study underscores the complex relationship between social dynamics, attitudes, and patterns of language use, offering a comprehensive understanding of how these factors influence language preservation and vitality. As the linguistic landscape develops, cultivating positive attitudes and awareness among the younger generation is essential for the survival of the Hitu dialect of the Wakasihu language. Moreover, the results of this research have implications not only for the Wakasihu community but also for similar linguistic contexts around the world, and constitute a strong call for proactive steps to safeguard our linguistic heritage.
Crypto Assets in Unit-Linked Life Insurance
Authors: Svoboda Andreas 
Published: October 2023

This article deals with the question why establishing of cryptocurrencies as a direct asset class in unit-linked life insurance has not been possible so far. Similarly, crypto ETFs have played only a minor role in unit-linked life insurance to date.
Unit-linked life insurance could benefit from immense return opportunities as well as tax ad-vantages by incorporating crypto assets. However, on the one hand, the cryptocurrency market is hardly regulated worldwide thus far, which runs counter to the current legal requirements for unit linked life insurance. Listed crypto ETFs are still in their infancy, and they have not yet been approved in the European Union.
It can be assumed that cryptocurrencies will only gain relevance as direct investments of unit-linked life insurance once the legal regulation of this market has been completed. Until then, insurance companies will probably rely to a greater extent on crypto ETFs and crypto ETNs to realize the return opportunities associated with cryptocurrencies.
Thyroid hormone sensitivity and diabetes onset: a longitudinal cross-lagged cohort
Authors: Cui Cancan , Sui He , Wang Zhijia , Zhang Te , Zheng Jia , Yan Han , Li Qianyu , Mo Zhanhao , Liu Lin 
Published: October 2023

PurposeThyroid hormones sensitivity is a newly proposed clinical entity closely related with metabolic health. Prior studies have reported the cross-sectional relationship between thyroid hormones sensitivity and diabetes; however, the longitudinal association is unclear to date. We aimed to explore the relationship between impaired thyroid hormone sensitivity at baseline and diabetes onset using a cohort design.MethodsThis study enrolled 7283 euthyroid participants at the first visit between 2008 and 2009, and then annually followed until diabetes onset or 2019. Thyrotropin (TSH), free triiodothyronine (FT3) and free thyroxine (FT4) were measured to calculate thyroid hormone sensitivity by thyroid feedback quantile-based index (TFQI), Chinese-referenced parametric thyroid feedback quantile-based index (PTFQI), thyrotropin index (TSHI), thyrotroph thyroxine resistance index (TT4RI) and FT3/FT4 ratio. Cox proportional hazard model and cross-lagged panel analysis were used.ResultsThe mean baseline age was 44.2 ± 11.9 years, including 4170 (57.3%) male. During a median follow-up of 5.2 years, 359 cases developed diabetes. There was no significant association between thyroid hormones sensitivity indices and diabetes onset, and adjusted hazard ratios per unit (95% CIs) were 0.89 (0.65-1.23) for TFQI, 0.91 (0.57-1.45) for PTFQI, 0.95 (0.70-1.29) for TSHI, 0.98 (0.70-1.01) for TT4RI and 2.12 (0.17-5.78) for FT3/FT4 ratio. Cross-lagged analysis supported the temporal association from fasting glucose to impaired thyroid hormones sensitivity indices.ConclusionsOur findings could not demonstrate that thyroid hormones sensitivity status is a predictor of diabetes onset in the euthyroid population. Elevated fasting glucose (above 7.0 mmol/L) appeared to precede impaired sensitivity indices of thyroid hormones.
Repurposing HLA genotype data of renal transplant patients to prevent severe drug hypersensitivity reactions
Authors: Manson Lisanne E. N. , Delwig Sander J. , Drabbels Jos J. M. , Touw Daan J. , De Vries Aiko P. J. , Roelen Dave L. , Guchelaar Henk-Jan 
Published: October 2023

Introduction: Specific alleles in human leukocyte antigens (HLAs) are associated with an increased risk of developing drug hypersensitivity reactions induced by abacavir, allopurinol, carbamazepine, oxcarbazepine, phenytoin, lamotrigine, or flucloxacillin. Transplant patients are genotyped for HLA as a routine practice to match a potential donor to a recipient. This study aims to investigate the feasibility and potential impact of repurposing these HLA genotype data from kidney transplant patients to prevent drug hypersensitivity reactions.Methods: A cohort of 1347 kidney transplant recipients has been genotyped in the Leiden University Medical Center (LUMC) using next-generation sequencing (NGS). The risk alleles HLA-A*31:01, HLA-B*15:02, HLA-B*15:11, HLA-B*57:01, and HLA-B*58:01 were retrieved from the NGS data. Medical history, medication use, and allergic reactions were obtained from the patient’s medical records. Carrier frequencies found were compared to a LUMC blood donor population.Results: A total of 13.1% of transplant cohort patients carried at least one of the five HLA risk alleles and therefore had an increased risk of drug-induced hypersensitivity for specific drugs. HLA-A*31:01, HLA-B*15:02, HLA-B*57:01, and HLA-B*58:01 were found in carrier frequencies of 4.61%, 1.19%, 4.46%, and 3.35% respectively. No HLA-B*15:11 carrier was found. In total nine HLA-B*57:01 carriers received flucloxacillin and seven HLA-B*58:01 carriers within our cohort received allopurinol.Discussion: Our study shows that repurposing HLA genotype data from transplantation patients for the assignment of HLA risk alleles associated with drug hypersensitivity is feasible. The use of these data by physicians while prescribing drugs or by the pharmacist when dispensing drugs holds the potential to prevent drug hypersensitivity reactions. The utility of this method was highlighted by 13.1% of the transplant cohort patients carrying an actionable HLA allele.
Unraveling the prognostic significance and molecular characteristics of tumor-infiltrating B lymphocytes in clear cell renal cell carcinoma through a comprehensive bioinformatics analysis
Authors: Yue Youwei , Cai Xinyi , Lu Changhao , Sechi Leonardo Antonio , Solla Paolo , Li Shensuo 
Published: October 2023

IntroductionClear cell renal cell carcinoma (ccRCC) is a prevalent subtype of kidney cancer that exhibits a complex tumor microenvironment, which significantly influences tumor progression and immunotherapy response. In recent years, emerging evidence has underscored the involvement of tumor-infiltrating B lymphocytes (TIL-Bs), a crucial component of adaptive immunity, and their roles in ccRCC as compared to other tumors. Therefore, the present study endeavors to systematically explore the prognostic and molecular features of TIL-Bs in ccRCC.MethodsInitially, xCell algorithm was used to predict TIL-Bs in TCGA-KIRC and other ccRCC transcriptomic datasets. The Log-Rank test and Cox regression were applied to explore the relationship of B-cells with ccRCC survival. Then, we used WGCNA method to identify important modules related to TIL-Bs combining Consensus subcluster and scRNA-seq data analysis. To narrow down the prospective biomarkers, a prognostic signature was proposed. Next, we explored the feature of the signature individual genes and the risk-score. Finally, the potential associations of signature with clinical phenotypes and drugs were investigated.ResultsPreliminary, we found ccRCC survival was negatively associated with TIL-Bs, which was confirmed by other datasets. Afterwards, ten co-expression modules were identified and a distinct ccRCC cluster was subsequently detected. Moreover, we assessed the transcriptomic alteration of B-cell in ccRCC and a relevant B-cell subtype was also pinpointed. Based on two core modules (brown, red), a 10-gene signature (TNFSF13B, SHARPIN, B3GAT3, IL2RG, TBC1D10C, STAC3, MICB, LAG3, SMIM29, CTLA4) was developed in train set and validated in test sets. These biomarkers were further investigated with regards to their differential expression and correlation with immune characteristics, along with risk-score related mutations and pathways. Lastly, we established a nomogram combined tumor grade and discovered underlying drugs according to their sensitivity response.DiscussionIn our research, we elucidated the remarkable association between ccRCC and B-cells. Then, we detected several key gene modules, together with close patient subcluster and B-cell subtype,which could be responsible for the TIL-Bs in ccRCC. Moreover, we proposed a 10-gene signature and investigated its molecular features from multiple perspectives. Overall, understanding the roles of TIL-Bs could aid in the immunotherapeutic approaches for ccRCC, which deserve further research to clarify the implications for patient prognosis and treatment.
Direct interaction of the molecular chaperone GRP78/BiP with the Newcastle disease virus hemagglutinin-neuraminidase protein plays a vital role in viral attachment to and infection of culture cells
Authors: Han Chenxin , Xie Ziwei , Lv Yadi , Liu Dingxiang , Chen Ruiai 
Published: October 2023

IntroductionGlucose Regulated Proteins/Binding protein (GRP78/Bip), a representative molecular chaperone, effectively influences and actively participates in the replication processes of many viruses. Little is known, however, about the functional involvement of GRP78 in the replication of Newcastle disease virus (NDV) and the underlying mechanisms.MethodsThe method of this study are to establish protein interactomes between host cell proteins and the NDV Hemagglutinin-neuraminidase (HN) protein, and to systematically investigate the regulatory role of the GRP78-HN protein interaction during the NDV replication cycle.ResultsOur study revealed that GRP78 is upregulated during NDV infection, and its direct interaction with HN is mediated by the N-terminal 326 amino acid region. Knockdown of GRP78 by small interfering RNAs (siRNAs) significantly suppressed NDV infection and replication. Conversely, overexpression of GRP78 resulted in a significant increase in NDV replication, demonstrating its role as a positive regulator in the NDV replication cycle. We further showed that the direct interaction between GRP78 and HN protein enhanced the attachment of NDV to cells, and masking of GRP78 expressed on the cell surface with specific polyclonal antibodies (pAbs) inhibited NDV attachment and replication.DiscussionThese findings highlight the essential role of GRP78 in the adsorption stage during the NDV infection cycle, and, importantly, identify the critical domain required for GRP78-HN interaction, providing novel insights into the molecular mechanisms involved in NDV replication and infection.