bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formación científica y dirección del proceso de enseñanza-aprendizaje. Resultados y desafíos
Authors: León García Margarita , Guzmán Huayamave Kennya 
Published: January 2025

El artículo expone los resultados finales del proyecto de investigación que tuvo como objetivo general caracterizar la dirección del proceso de enseñanza aprendizaje y su correspondencia con la formación científica de los estudiantes de la Universidad Laica VICENTE ROCAFUERTE de Guayaquil, como vía importante para el logro de una inserción favorable de estos en la vida laboral. En el desarrollo del proyecto se estudiaron, analizaron y asumieron las posiciones teóricas que permitieron conceptualizar las variables en estudio y operacionalizarlas, lo que permitió identificar la situación inicial de la formación científica de los estudiantes en la Universidad Laica VICENTE ROCAFUERTE de Guayaquil, así como la dirección del proceso de enseñanza aprendizaje para favorecerla. El resultado mostró que tanto una como la otra variable, se encuentran en niveles medios de desarrollo ya que las respuestas se concentran en opciones que favorece poco o no favorece. El desafío que enfrenta la universidad es preparar a los docentes de todas las asignaturas para una óptima dirección del proceso de enseñanza aprendizaje de modo que, además de atender las variables relacionadas con resultados de aprendizaje específicos, también se atienda la formación científica desde el salón de clase o desde la práctica pre-profesional, aprovechando también las potencialidades que brinda la plataforma de la universidad, para dicha preparación. Para contribuir al logro de este desafío, se ha elaborado y validado una guía metodológica dirigida a docentes, que por la vía de la auto-preparación, los capacite para una mejor dirección del proceso de enseñanza aprendizaje.
Konseling Individu Dengan Teknik Relaksasi Untuk Mereduksi Panic Attack Terhadap Hasil Belajar (Studi Kasus Klien “W” Mahasiswa UIN Raden Fatah Palembang)
Authors: Ardila Rita , Razzaq Abdur , Jannati Zhila 
Published: January 2025

Panic attack adalah suatu serangan panik ini bisa terjadi kapan saja, baik karena ada faktor pemicu maupun tanpa faktor pemicu. Penelitian ini bertujuan untuk mengetahui gambaran panic attack klien “W” sebelum dilakukan konseling individu dengan teknik relaksasi, mengetahui faktor yang memperngaruhi perilaku panic attack klien “W” , mengetahui bagaimana penerapan konseling individu dengan teknik relaksasi dalam mereduksi panic attack klien “W”, mengetahui perilaku panic attack klien “W” setelah dilakukan konseling individu dengan teknik relaksasi. Penelitian ini menggunakan pendekatan kualitatif dengan metode penelitian studi kasus. Subjek dalam penelitian ini yaitu klien “W”. teknik pengumpulan data adalah menggunakan observasi, wawancara, dan dokumentasi. Sedangkan teknik analisis data menggunakan reduksi data, data display, dan penarik kesimpulan. Adapun dari hasil penelitian ini menunjukkan bahwa sebelum dilakukan konseling individu masih berada dalam katagori tinggi, dimana dapat dilihat dari beberapa perilaku yang dilakukan oleh klien “W” seperti memiliki rasa takut, tidak bisa mengontrol panic attack, memiliki kesulitan dalam berpikir saat panic attack menyerang. Penerapan konseling individu dilakukan konseling individu dilakukan dalam enak kali pertemuan dimana setiap pertemuan menggunakan tiga tahapan yaitu tahap awal, tahap inti atau kerja dan tahap akhir. Gambaran setelah dilakukan konseling individu dengan teknik relaksasi yaitu sudah mengalami penurunan dimana klien “W” sudah tidak lagi melakukan hal-hal seperti tidak menolak ketika disuruh dan lebih bisa menyikapi hal-hal negatif menjadi positif.
Biomedical Metal–Organic Framework Materials: Perspectives and Challenges
Authors: Wang Alec , Walden Madeline , Ettlinger Romy , Kiessling Fabian , Gassensmith Jeremiah J. , Lammers Twan , Wuttke Stefan , Peña Quim 
Published: November 2023

AbstractMetal–organic framework (MOF) materials are gaining significant interest in biomedical research, owing to their high porosity, crystallinity, and structural and compositional diversity. Their versatile hybrid organic/inorganic chemistry endows MOFs with the capacity to retain organic (drug) molecules, metals, and gases, to effectively channel electrons and photons, to survive harsh physiological conditions such as low pH, and even to protect sensitive biomolecules. Extensive preclinical research has been carried out with MOFs to treat several pathologies and, recently, their integration with other biomedical materials such as stents and implants has demonstrated promising performance in regenerative medicine. However, there remains a significant gap between MOF preclinical research and translation into clinically and societally relevant medicinal products. Here, the intrinsic features of MOFs are outlined and their suitability to specific biomedical applications such as detoxification, drug and gas delivery, or as (combination) therapy platforms is discussed. Furthermore, relevant examples of how MOFs have been engineered and evaluated in different medical indications, including cancer, microbial, and inflammatory diseases is described. Finally, the challenges facing their translation into the clinic are critically examined, with the goal of establishing promising research directions and more realistic approaches that can bridge the translational gap of MOFs and MOF‐containing (nano)materials.
Stretchable Electronic Facial Masks for Skin Electroporation
Authors: Xu Xinkai , Guo Liang , Liu Hao , Zhou Zanxin , Li Shuang , Gu Qi , Ding Shenglong , Guo Haitao , Yan Yan , Lan Yuqun , Li Qinlan , Wei Wanxin , Zhang Jian , Liu Chong , Su Yewang 
Published: November 2023

AbstractA stretchable electronic facial mask (SEFM) for skin electroporation by transdermal drug delivery is introduced. The SEFM exhibits characteristics of reusability, water‐resistant, low cost, and portability. To achieve these objectives while maintaining satisfactory mechanical and electrical performance of the device, two main areas are innovated: (1) Grinding and doping modification of conductive ink to enhance its adhesion to silicone and increase conductivity through material research, as well as mechanical structure design for stretching stability of conductive ink electrodes. (2) Doping modification of high‐temperature vulcanizes silicone to address the issue of increased resistance during encapsulation due to the swell of conductive ink. The SEFM designed with these innovations, is validated in rat experiments, demonstrating a 3–4 times increase in drug intake compared to the control group without skin electroporation. Safety is confirmed, and the promotion of niacinamide drug delivery is verified in human volunteers. The materials‐modified conductive ink and conductive silicone proposed in SEFM can be employed in other flexible wearable devices, and this platform has the potential for future integration of additional components to achieve expanded functionality and productization.
Reversible Structural Evolution of Metal‐Nitrogen‐Doped Carbon Catalysts During CO2 Electroreduction: An Operando X‐ray Absorption Spectroscopy Study
Authors: Hursán Dorottya , Timoshenko Janis , Ortega Eduardo , Jeon Hyo Sang , Rüscher Martina , Herzog Antonia , Rettenmaier Clara , Chee See Wee , Martini Andrea , Koshy David , Roldán Cuenya Beatriz 
Published: November 2023

AbstractElectrochemical CO2 reduction (CO2RR) is a rising technology, aiming to reduce the energy sector dependence on fossil fuels and to produce carbon‐neutral raw materials. Metal‐nitrogen‐doped carbons (M‐N‐C) are emerging, cost‐effective catalysts for this reaction; however, their long‐term stability is a major issue. To overcome this, understanding their structural evolution is crucial, requiring systematic in‐depth operando studies. Here a series of M‐N‐C catalysts (M = Fe, Sn, Cu, Co, Ni, Zn) is investigated using operando X‐ray absorption spectroscopy. It is found that the Fe‐N‐C and Sn‐N‐C are prone to oxide clusters formation even before CO2RR. In contrast, the respective metal cations are singly dispersed in the as‐prepared Cu‐N‐C, Co‐N‐C, Ni‐N‐C, and (Zn)‐N‐C. During CO2RR, metallic clusters/nanoparticles reversibly formed in all catalysts, except for the Ni‐N‐C. This phenomenon, previously observed only in Cu‐N‐C, thus is ubiquitous in M‐N‐C catalysts. The competition between M‐O and M‐N interactions is an important factor determining the mobility of metal species in M‐N‐C. Specifically, the strong interaction between the Ni centers and the N‐functional groups of the carbon support results in higher stability of the Ni single‐sites, leading to the excellent performance of Ni‐N‐C in the CO2 to CO conversion, in comparison to other transition metals.
Employing Noble Metal–Porphyrins to Engineer Robust and Highly Active Single‐Atom Nanozymes for Targeted Catalytic Therapy in Nasopharyngeal Carcinoma
Authors: Wang Daji , Wang Jie , Gao Xuejiao J. , Ding Hui , Yang Ming , He Zhiheng , Xie Jiaying , Zhang Zixia , Huang Haibing , Nie Guohui , Yan Xiyun , Fan Kelong 
Published: November 2023

AbstractSingle‐atom nanozymes (SANzymes) emerge as promising alternatives to conventional enzymes. However, chemical instability limits their application. Here, a systematic synthesis of highly active and stable SANzymes is presented by leveraging noble metal–porphyrins. Four noble metal–porphyrins are successfully synthesized to mimic the active site of natural peroxidases through atomic metal–N coordination anchored to the porphyrin center. These noble metal–porphyrins are integrated into a stable and biocompatible Zr‐based metal–organic framework (MxP, x denoting Ir, Ru, Pt, and Pd). Among these, MIrP demonstrates superior peroxidase‐like activity (685.61 U mg−1), catalytic efficiency, and selectivity compared to horseradish peroxidase (267.71 U mg−1). Mechanistic investigations unveil heightened catalytic activity of MIrP arises from its robust H2O2 adsorption capacity, unique rate‐determining step, and low energy threshold. Crucially, MIrP exhibits remarkable chemical stability under both room temperature and high H2O2 concentrations. Further, through modification with (−)‐Epigallocatechin‐3‐Gallate, a natural ligand for Epstein–Barr virus (EBV)‐encoded latent membrane protein 1, targeted SANzyme (MIrPHE) tailored for EBV‐associated nasopharyngeal carcinoma is engineered. This study not only presents an innovative strategy for augmenting the catalytic activity and chemical stability of SANzymes but also highlights the substantial potential of MIrP as a potent nanomedicine for targeted catalytic tumor therapy.
Tele‐proctoring for minimally invasive surgery across Japan: An initial step toward a new approach to improving the disparity of surgical care and supporting surgical education
Authors: Takemasa Ichiro , Okuya Koichi , Okita Kenji , Akizuki Emi , Miyo Masaaki , Ishii Masayuki , Miura Ryo , Ichihara Momoko , Takahiro Korai , Oki Eiji , Takatsuki Mitsuhisa , Eguchi Susumu , Ichikawa Daisuke , Kitagawa Yuko , Sakai Yoshiharu , Mori Masaki 
Published: November 2023

AbstractAimThe aim of this study was to verify the clinical feasibility of tele‐proctoring using our ultra‐low latency communication system with shared internet access.MethodsConnections between two multiple remote locations at various distances were established through the TELEPRO® tele‐proctoring system. The server records the latency between the two locations for tele‐proctoring using the annotations. Questionnaires were administered to the surgeons, assistants, and medical staff. Respondents rated the quickness and quality of communication in terms of latency and disturbances in the audio, video, and usefulness of the live telestrations with annotation.ResultsSeven hospitals tele‐proctored with Sapporo Medical University between January 2021 and September 2022. The median latency of annotation between the two locations ranged from 24.5 to 48.5 ms. No major technological problems occurred, such as streaming interruption, loss of video or audio, poor resolution. The video encoding time was 10 ms, and its decoding time was 0.8 ms. The total latency positively correlated with the distance between two locations (R = 0.55, p < 0.01). The quality of communication regarding latency, disturbance, and surgical education with intraoperative annotative instructions showed similar trends, with perfectly fine being the most common response. No significant differences in surgical quality, educational effect, or social impact were observed between the latency ≥30 and <30 ms groups for whether the size of latency affects surgical education.ConclusionThe feasibility of the tele‐proctoring system is expected to be a sustainable approach to help education for young surgeons and surgical supports in rural areas, thereby reducing disparities in health care.
Enzymatic Dimerization‐Induced Self‐Assembly of Alanine‐Tyramine Conjugates into Versatile, Uniform, Enzyme‐Loaded Organic Nanoparticles
Authors: Kumar Pradhan Manas , Suresh Puthenpurackal Surya , Srivastava Aasheesh 
Published: November 2023

AbstractHerein, we report a novel enzymatic dimerization‐induced self‐assembly (e‐DISA) procedure that converts alanine‐tyramine conjugates into highly uniform enzyme‐loaded nanoparticles (NPs) or nanocontainers by the action of horseradish peroxidase (HRP) in an aqueous medium under ambient conditions. The NP formation was possible with both enantiomers of alanine, and the average diameter could be varied from 150 nm to 250 nm (with a 5–12 % standard deviation of as‐prepared samples) depending on the precursor concentration. About 60 % of the added HRP enzyme was entrapped within the NPs and was subsequently utilized for post‐synthetic modification of the NPs with phenolic compounds such as tyramine or tannic acid. One‐pot multi‐enzyme entrapment of glucose oxidase (GOx) and peroxidase (HRP) within the NPs was also achieved. These GOx‐HRP loaded NPs allowed multimodal detection of glucose, including that present in human saliva, with a limit of detection (LoD) of 740 nM through fluorimetry. The NPs exhibited good cytocompatibility and were stable to changes in pH (acidic to basic), temperature, ultrasonication, and even the presence of organic solvent (EtOH) to a certain extent, since they are stabilized by intermolecular hydrogen bonding, π‐π, and CH‐π interactions. The proposed e‐DISA procedure can be widely expanded through the design of diverse enzyme‐responsive precursors.
Divergent Chemo‐ and Biocatalytic Route to 16β‐Methylcorticoids: Asymmetric Synthesis of Betamethasone Dipropionate, Clobetasol Propionate, and Beclomethasone Dipropionate
Authors: Wei Jianhai , Zhang Yajiao , Liu Minjie , Ning Yingtang , Cao Yiran , Chen Fen‐Er 
Published: November 2023

Abstract16β‐Methylcorticoids are among the most important glucocorticoid steroids for the treatment of various dermatological disorders, respiratory infections, and other allergic reactions elicited during inflammatory responses of the human body. Betamethasone dipropionate, clobetasol propionate, and beclomethasone dipropionate are particularly noteworthy for their synthetic intractability. Despite five decades of research, these 16β‐methylcorticoids have remained challenging synthetic targets owing to insurmountable issues of reactivity, selectivity, and cost efficiency associated with all previously explored strategies. We herein report our practicability‐oriented strategy toward the unified stereoselective synthesis of 16β‐methylcorticoids in 12.6–14.0 % overall yield from commercially available 9α‐hydroxyandrost‐4‐ene‐3,17‐dione (9α‐OH‐AD). In this approach, the chiral C16β‐Me and C17α‐OH groups of the corticosteroid D ring were installed via a substrate‐controlled diastereo‐ and enantioselective Mn‐catalyzed oxidation‐reduction hydration of Δ4,9(11),16‐triene‐3,20‐dione. The C1−C2 double bond of the corticosteroid A ring was constructed using an unprecedented engineered 3‐ketosteroid‐Δ1‐dehydrogenase (MK4‐KstD)‐catalyzed regioselective Δ1‐dehydrogenation of Δ4,9(11)‐diene‐3,21‐dione. This strategy provides a general method and a key precursor for the divergent synthesis of a variety of glucocorticoids and related steroidal drugs.
Analysis of the mechanism of antioxidant synergism between α‐tocopherol and myricetin in bulk oil
Authors: Bayram Ipek , Decker Eric A. 
Published: November 2023

Abstractα‐Tocopherol (α‐TOC) and myricetin (MYR) synergistically inhibit lipid oxidation in bulk oil but the mechanism underlying this effect is unknown. In this research, stripped soybean oil (SSO) was treated with α‐tocopherol (50 μM), myricetin (10–250 μM), and their combinations. Taxifolin (TAX) was also tested because it has structural similarities to myricetin but with a higher redox potential. α‐Tocopherol: myricetin ratios of 5:1, 2:1, 1:1, 1:2, and 1:5 resulted in extended lag phases ranging from 16 to 99 days, with lag phase increasing with increasing myricetin concentrations. Synergism between α‐tocopherol and myricetin was also observed in phospholipid‐containing bulk oils both in the absence and presence of reverse micelles, although the reverse micelles shortened the lag phases. Myricetin (redox potential = 360 mV) delayed the oxidation of α‐tocopherol (redox potential = 500 mV) whereas taxifolin (redox potential = 500 mV) did not. Both myricetin and taxifolin were able to chelate iron as determined by UV–VIS spectroscopy. These results suggested that the lower redox potential of myricetin allowed it to produce synergistic antioxidant activity potentially by regenerating oxidized α‐tocopherol and through its ability to decrease oxidation by metal chelation.
Population structure of the critically endangered Brazilian guitarfish Pseudobatos horkelii (Rhinobatidae) revealed by double‐digest restriction site‐associated DNA sequencing
Authors: Torres Yan , Rotundo Matheus Marcos , Vianna Marcelo , Charvet Patricia , Faria Vicente Vieira , Oliveira Claudio , Foresti Fausto , Cruz Vanessa Paes 
Published: November 2023

Abstract

Molecular analysis is a powerful tool for assessing and developing conservation strategies for endangered species. Elasmobranchs comprise one of the most threatened groups of vertebrates, with about one‐third of the species subject to some degree of threat. This is the case for Pseudobatos horkelii, the Brazilian guitarfish, which has suffered severe population reductions (>80%) in recent decades, mainly through coastal overfishing. Understanding the genetic diversity of this species is paramount to applying adequate management and conservation strategies.
In this context, the present study evaluated the genetic diversity of P. horkelii, employing single‐nucleotide polymorphism (SNP) nuclear markers obtained by double‐digest restriction site‐associated DNA sequencing (ddRADseq). Samples collected in three areas along the Brazilian Coast (Rio de Janeiro, São Paulo, and Rio Grande do Sul) provided an average of 1,565,339 reads and 2,846 SNPs.
The results indicate the presence of at least two genetically distinct populations along the Brazilian Coast, with molecular diversity differences noted among clusters. The heterozygosity excess observed in all populations is likely the result of a bottleneck, probably resulting from overfishing and habitat fragmentation. Moreover, the upwelling phenomenon in Cabo Frio, in the state of Rio de Janeiro, may have a significant influence over the observed genetic structure.
The gene flow between the clusters was estimated and was noted as asymmetric between the populations studied. The observed patterns may be the result of the combination of biological characteristics and overfishing pressure in the past decades. The evidence of structured populations presenting genetic diversity differences provides insightful information for the development of stock management and conservation strategies for the Brazilian guitarfish.
AARS2as a novel biomarker for prognosis and its molecular characterization in pan‐cancer
Authors: Liu Long , Gao Jie , Liu Xudong , Zhang Feng , Hu Bowen , Zhang Huapeng , Wang Zhihui , Tang Hongwei , Shi Ji Hua , Zhang Shuijun 
Published: November 2023

AbstractIntroductionThe mitochondrial alanyl‐tRNA synthetase 2 (AARS2) as one of aminoacyl‐tRNA synthases (ARSs) performs amino acid transportation and involves protein synthesis. However, its role in cancer remains largely unexplored.MethodsIn this study, more than 10,000 samples were enrolled to explore genomic alterations, biological function, prognosis, and clinical treatment based on AARS2 across pan‐cancer. The molecular characterization of AARS2 was confirmed in hepatocellular carcinoma (HCC) using proteomics analysis, quantitative real‐time PCR, western blotting, immunohistochemical staining, and cell experiments.ResultsFor genomic landscape, the AARS2 was dramatically upregulated in multiple cancers, which might be mainly caused by copy number alteration rather than mutation and methylation. The abnormal expression of AARS2 was prominently associated with activity of cancer pathways and performed oncogenic roles in most cancers. Systematic experiments in vitro substantiated the elevated expression of AARS2, and the deficiency of it inhibited cell proliferation and cell migration in HCC. Meanwhile, our findings suggested that AARS2 could serve as a novel promising and stable biomarker for assessing prognosis and immunotherapy. Moreover, a variety of therapeutic drugs and targeted pathways were proposed for cancer treatment, which might enhance clinical efficacy.ConclusionThe AARS2 could serve as a new oncogenic gene that promotes cell proliferation and migration in HCC. The comprehensive investigations increased the understanding of AARS2 across human cancers and generated beginning insights of AARS2 in genomic landscape, molecular biological function, prognosis, and clinical treatment.
Enlighting Electron Routes In Oxyfunctionalizing Synechocystis sp. PCC 6803
Authors: Tüllinghoff Adrian , Toepel Jörg , Bühler Bruno 
Published: November 2023

AbstractPhototrophic microorganisms, like cyanobacteria, are gaining attention as host organisms for biocatalytic processes with light as energy source and water as electron source. Redox enzymes, especially oxygenases, can profit from in‐situ supply of co‐substrates, i. e., reduction equivalents and O2, by the photosynthetic light reaction. The electron transfer downstream of PS I to heterologous electron consuming enzymes in principle can involve NADPH, NADH, and/or ferredoxin, whereas most direct and efficient transfer is desirable. Here, we use the model organism Synechocystis sp. PCC 6803 to investigate, to what extent host and/or heterologous constituents are involved in electron transfer to a heterologous cytochrome P450 monooxygenase from Acidovorax sp. CHX100. Interestingly, in this highly active light‐fueled cycloalkane hydroxylating biocatalyst, host‐intrinsic enzymes were found capable of completely substituting the function of the Acidovorax ferredoxin reductase. To a certain extent (20 %), this also was true for the Acidovorax ferredoxin. These results indicate the presence of a versatile set of electron carriers in cyanobacteria, enabling efficient and direct coupling of electron consuming reactions to photosynthetic water oxidation. This will both simplify and promote the use of phototrophic microorganisms for sustainable production processes.