bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Abdomen in Crohn’s disease patients: short- and long-term outcomes
Authors: Opincans Janis , Rudzats Agris , Kecbaja Zurabs , Kaminskis Aleksejs , Ivanovs Igors , Iesalnieks Igors 
Published: October 2023

Abstract
Background. To determine long-term outcome in Crohn’s disease patients hospitalized for acute abdominal pain. Methods. Retrospective chart review of consecutive Crohn’s disease patients hospitalized for acute abdominal pain at the tertiary referral centre in Eastern Europe. Results. Between 2015 and 2020, 65 patients with Crohn’s disease were admitted at least once to hospitals’ emergency room because of acute abdominal pain. 13 patients were admitted twice, 8 three times, 3 patients four times and 3 patients five times. Also, the intake of immunosuppressive medication was apparently rare, thus, most patients have been treated by 5-ASA medication only. Most patients had ileal involvement. Two patients presented with an intraabdominal abscess. 53 of 65 patients underwent abdominal imaging during the hospitalization. After hospital admission, 45 patients were treated with antibiotics (65%),17 with steroids (17%), 8 patients received a nasogastric tube and 3 were put on parenteral nutrition. Six patients underwent an intestinal resection during the initial hospitalization (9%). The length of the hospitalization was median 8 days (range, 1 to 115) and it was significantly longer in those, who underwent surgery (mean 28 days vs. 9 days, p = 0.003). Follow-up information was obtained for all patients, and the median follow-up time was 42.8 months. During the follow-up period, 26 patients (40%) readmitted for abdominal emergencies. 14 patients underwent surgery – either at the initial or later hospitalization (21%). Thirteen out of 14 surgeries were emergencies, three were concluded by ostomy formation (21%). One patient developed postoperative intraabdominal complication, however, two patients died postoperatively (14%). By multivariate analysis, complicated disease (stricturing or penetrating) was associated with hospital readmission (Hazard ratio, 5.8, 95% CI: 1.4–23.3; p = 0.013). An elevated C-reactive protein level was associated with a decreased risk of hospital readmission (Hazard ratio,0.08, 95% CI: 0.01–0.55; p = 0.010). Also, complicated disease (stricturing or penetrating) was associated with enhanced probability to undergo intestinal surgery (Hazard ratio, 10.3, 95% CI: 2.4–44.1; p = 0.002) Conclusion. Ileocolic disease is the main cause of hospitalization for acute abdominal pain. Complicated (structuring or penetrating) disease should be diagnosed early and surveilled closely.
Annexin A1 conveys neuroprotective function via inhibiting oxidative stress in diffuse axonal injury of rats
Authors: Zheng Fengwei , Li Weixin , Su Shaobo , Hui Qiaoyan 
Published: October 2023

Abstract
Purpose
Diffuse axonal injury (DAI) is a critical pathological facet of traumatic brain injury (TBI). Oxidative stress plays a significant role in progress of DAI. Annexin A1 has been demonstrated benefit for recovery of neurofunctional outcomes after TBI. However, whether annexin A1 exhibits neuronal protective function through modulating oxidative stress in DAI remains unknown.
Methods
Expression of annexin A1 was evaluated via real time PCR and western blot analysis in rat brainstem after DAI. Neurological effect of annexin A1 following DAI through quantification of modified neurologic severity score (mNSS) was compared between wild-type and annexin A1-knockout rats. Brain edema and neuronal apoptosis, as well as expression of oxidative factors and inflammatory cytokines was analysed between wild-type and annexin A1 deficiency rats after DAI. Furthermore, mNSS, oxidative and inflammatory cytokines were assayed after timely administration of recombinant annexin A1 for DAI rats.
Results
In brainstem of DAI, the expression of annexin A1 remarkably increased. Ablation of annexin A1 increased mNSS score and brain water content of rats after DAI. Neuron apoptosis in brainstem after DAI was exaggerated by annexin A1 deficiency. In addition, annexin A1 deficiency significantly upregulated level of oxidative and inflammatory factors in brainstem of DAI rats. Moreover, mNSS decreased by annexin A1 treatment in rats following DAI. Expression of oxidative and inflammatory molecules in rat brainstem subjected to DAI inhibited by annexin A1 administration.
Conclusions
These results demonstrated that annexin A1 exhibits neuronal protective function in the progression of DAI mainly dependent on suppressing oxidative stress and inflammation.
Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus
Authors: Luo Laifu , Chen Chen , Ji Liqin , Wu Congcong , Li Wei , Liu Xiaoli , Hong Xiaoyou , Zhu Xinping , Zhang Jiping 
Published: October 2023

Abstract

A total of 42 polymorphic simple sequence repeat (SSR) markers have been developed and characterized from genome sequences of Mastacembelus armatus to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen, observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci signiﬁcantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for genetic conservation and marker-assisted breeding for M. armatus.
Subspace algorithms for detecting a definite Hermitian matrix pair
Authors: Pandur Marija Miloloža 
Published: October 2023

Abstract
The Hermitian pair (A,B) is called definite if some real linear combination of the matrices A and B is a positive definite matrix. There are several reasons why it is important to detect whether a given matrix pair is definite. Determining whether a given matrix pair is definite is not straightforward. There exist different kinds of algorithms for that task. Most of them are not appropriate for medium-size or large-size matrix pairs. We propose subspace algorithms for medium-size or large-size matrix pairs that are based on iterative testing of small compressed Hermitian matrix pairs formed by using subspaces of small dimensions. First, we propose a new basic subspace algorithm for detecting definite matrix pairs. Furthermore, we propose a specialized algorithm and its preconditioned variant. In this preconditioned variant, we need to solve several systems of linear equations. These systems can be solved only approximately and not necessarily in every iteration step. Numerical experiments demonstrate the efficiency of our specialized algorithm.
Micro analysis of rock fracturing under the impact of high-temperature and high-pressure fluid
Authors: Pang Shuogang , Hu Shaobin , Yan Zhengyong , Cai Yukang 
Published: October 2023

Abstract
To reveal the mechanism of high-temperature and high-pressure fluid thermal shock rock breakage and the formation mechanism of microcracks, this paper carried out an experimental study of supercritical (SC)-CO2 thermal shock rock breakage by using a self-developed hard rock true triaxial aerodynamic pulse fracturing experimental system and studied the micro-characteristics of different kinds of cracks after high-temperature and high-pressure fluid impact cracking by scanning electron microscopy. This study discovered three main actions in the process of rock breaking by SC-CO2 thermal shock. 1) The stress wave generated by thermal shock forms radial tensile stress and radial cracks during stress wave propagation and circumferential tensile stress and circumferential cracks during unloading. At the same time, spherical particles appear on the fracture surfaces of radial cracks and circumferential cracks; these particles are features of vertical and horizontal gullies. 2) The splitting action of high-temperature and high-pressure fluid further expands radial and circumferential cracks and promotes the generation of shear cracks (branch cracks). The fracture surfaces of branch cracks appear flat and smooth and have bedded and river shapes. 3) Collapse pits are formed by scouring. Although the collapse pits have different shapes and depths, all the pits are flat and smooth. The research results are significant for revealing the rock breaking mechanism of SC-CO2 thermal shock and play an important role in quantitatively controlling directional pre-cracking, hole layout parameter design, and crack type, size and complexity.
Evaluating Intra and Inter-observer bias in the Cosmetic rating for random vs. serial assessment of Breast photographs
Authors: Belani Preeti , Pathak Rima , Kenekar Shraddha , Pokale Gaurika , Rane Pallavi , Chalke Ashwini , Wadasadawala Tabassum 
Published: October 2023

Abstract

BACKGROUND
This study was done to assess inter and intra-rater bias in rating of cosmesis, when breast photographs were evaluated serially or randomly by a panel of six members having varying years of experience.
METHODS
Cosmetic assessment was done subjectively for 175 images [of 50 unilateral breast cancer patients for whom at least 3 images were collected], that were arranged serially from baseline to follow up in chronological order termed ‘serial assessment setting’ [SAS]. For ‘random assessment setting’ [RAS], all images was randomly arranged for assessment. Objectively assessment was also done using BCCT.core. Kappa index was calculated for agreement between the RAS and SAS rating for the 3 panellists’ groups and with BCCT.core.
RESULTS
Good agreement [kappa 0.659] was found between the mean panel cosmetic scores for both SAS and RAS. Fair agreement was found when subjective RAS [k=0.301] and SAS [k=0.343] scores were compared with the BCCT.core, which was highest for the most experienced panellists with SAS k=0.387 and RAS k=0.436. Both SAS and RAS had good intra-rater reliability.
CONCLUSIONS
SAS improves the agreement with BCCT.core rating and may be used if validated in a larger cohort. The clinical experience of the panellist impacts cosmetic rating and must be considered before forming a panel.
Epidemiological investigation of equine herpesvirus in large-scale donkey farms in Liaocheng area and its effect on immunity and antioxidant capacity
Authors: Ji Yanfei , Xu Dandan , Zhao Xia , Liu Wenqiang 
Published: October 2023

Abstract
Background
Equine herpesvirus (EHV) may cause respiratory, reproductive and neurological diseases in equine animals, including donkeys, of which EHV type 1 (EHV-1) and EHV-4 are the main pathogens. To investigate the prevalence of EHV in donkeys in the Liaocheng area (an important donkey breeding areas in China), and the effect of EHV infection on immunity and antioxidant capacity in donkeys.the epidemiological investigation of EHV was proceed by detected the EHV-1 and EHV-4 in 230 donkey serum collected from 27 large-scale donkey farms, and the serum samples of 10 normal (without EHV-infected) and 10 EHV-infected donkeys with similar age were used to detect the immune and antioxidant related indexs.
Result
The results showed that 62.96% (17/27) of farms were positive for EHV, and the total positive rate of EHV was 12.61% (29/230) with 7.82% (18/230) for EHV-1, 9.57% (22/230) for EHV-4 .Conclusion: Moreover, The differences of EHV infection among different regions in Liaocheng were not significant (P > 0.05), but the highest positive rate was found in Dong'e County (21.28%,10/47). The highest positive rate of EHV-1/4 was 21.18% (18/85) in adult donkeys aged 1–4 years, which was significantly different from other age groups (P < 0.05). The difference in seasons was not significant (P > 0.05), but the highest positive rate was 19.61% (10/51) in winter. Furthermore, EHV infection increased the levels of the immune related indexs IgA, IgG, IgM and IL-6, inhibited the activities of SOD and T-AOC, and improve the activities of GSH-PX and content of MDA.
Consclusions:
These results indicated that EHV infection is prevalent in large-scale donkey farms in the Liaocheng area, and EHV infection could interfere immune, cause inflammation and oxidative stress, which facilitate early warning of EHV epidemic risk.
Clinical analysis of 116 children with Meckel’s diverticulum and review of the literature
Authors: Zhang TingTing , Zhang RuiYun , Cai Peng , Zhu Jie , Zhao HaoWei , Jiang YuLiang , Wang Qi , Zhu MengLei , Zhou XiaoGang , Hu FeiLong , Gu ZhiCheng , Zhu ZhenWei 
Published: October 2023

Abstract
Objective: We aimed to retrospectively analyze the clinical characteristics of pediatric Meckel’s diverticulum to improve the understanding and diagnosis of Meckel’s diverticulum in children.
Methods: The clinical data of 116 hospitalized children with Meckel’s diverticulum diagnosed by surgery in the Department of Pediatric Surgery, Children’s Hospital of Soochow University, between January 2017 and January 2023 were analyzed retrospectively.
Results: A total of 95 boys and 21 girls were included in this study, with a male-to-female ratio of 4.524:1. The observed clinical manifestations could be roughly divided into the following three categories: gastrointestinal bleeding in 68 cases (59%), intestinal obstruction in 35 cases (30%), and abdominal inflammation in 10 cases (9%). The positive rate of 99mTc-pertechnetate dynamic imaging (99TcmO4-ECT) was higher than that of abdominal ultrasound in all patients and patients with gastrointestinal bleeding. The sensitivity and specificity of 99TcmO4-ECT were 98% and 25%, respectively. The surgical methods of Meckel’s diverticulum are mainly divided into two types: either wedge resection of the diverticulum or resection of the diverticulum and adjacent bowel after laparoscopic or open exploration. The average distance between the diverticulum and ileocecal junction was 50.5 ± 18.2 cm, the average length of the diverticulum was 3.25 ± 1.6 cm, and the average width of the diverticulum was 1.52 ± 0.7 cm. According to the pathological examination results, the incidence of ectopic mucosal tissue in Meckel’s diverticulum in the gastrointestinal bleeding group was higher than that in the non-gastrointestinal bleeding group. The clinical symptoms of gastrointestinal bleeding in children with Meckel’s diverticulum are related to the presence of ectopic mucosal tissue.
Conclusions: Gastrointestinal bleeding is the most common clinical manifestation of Meckel’s diverticulum in children, and it is closely related to the presence of ectopic mucosal tissue. 99TcmO4-ECT is an important imaging examination for the diagnosis of Meckel’s diverticulum. Once Meckel’s diverticulum is suspected, immediate surgical resection is advocated.
Exosomes derived from IFNγ-stimulated mesenchymal stem cells protect photoreceptors in RCS rats by restoring immune homeostasis through tsRNAs
Authors: A Luodan , Qu Linghui , He Juncai , Ge Lingling , Gao Hui , Huang Xiaona , You Tianjing , Gong Hong , Liang Qingle , Chen Siyu , Xie Jing , Xu Haiwei 
Published: October 2023

Abstract
Background Retinitis pigmentosa is a neurodegenerative disease with major pathologies of photoreceptor apoptosis and immune imbalance. Mesenchymal stem cells (MSCs) have been approved for clinical application for treating various immune-related or neurodegenerative diseases. The objective of this research was to investigate the mechanisms underlying the safeguarding effects of MSC-derived exosomes in a retinal degenerative disease model.Results IFNγ-stimulated exosomes (IFNγ-Exos) secreted from MSCs were isolated, purified, and injected into the vitreous body of RCS rats on postnatal day (P) 21. Morphological and functional changes in the retina were examined at P28, P35, P42, and P49 in RCS rats. The mechanism was explored using high-throughput sequencing technology and confirmed in vitro. Treatment with IFNγ-Exo produced better protective effects on photoreceptors and improved visual function in RCS rats. IFNγ-Exo significantly suppressed the activated microglia and inhibited the inflammatory responses in the retina of RCS rats, which was also confirmed in the lipopolysaccharide-activated microglia cell line BV2. Furthermore, through tRNA-derived small RNA (tsRNA) sequencing, we found that IFNγ-Exos from MSCs contained higher levels of Other-1_17-tRNA-Phe-GAA-1-M3, Other-6_23-tRNA-Lys-TTT-3, and TRF-57:75-GLN-CGG-2-m2 than native exosomes, which mainly regulated inflammatory and immune-related pathways, including the mTOR signaling pathway and EGFR tyrosine kinase inhibitor resistance.Conclusion IFNγ stimulation enhanced the neuroprotective effects of MSC-derived exosomes on photoreceptors of the degenerative retina, which may be mediated by immunoregulatory tsRNAs acting on microglia.
Functional data geometric morphometrics with machine learning for craniodental shape classification in shrews
Authors: Pillay Aneesha Balachandran , Pathmanathan Dharini , Dabo-Niang Sophie , Abu Arpah , Omar Hasmahzaiti 
Published: October 2023

Abstract
This work proposes a functional data analysis (FDA) approach for morphometrics in classifying three shrew species (S. murinus, C. monticola and C. malayana) from Peninsular Malaysia. Functional data geometric morphometrics (FDGM) for 2D landmark data is introduced and its performance is compared with classical geometric morphometrics (GM). The FDGM approach converts 2D landmark data into continuous curves, which are then represented as linear combinations of basis functions. The landmark data was obtained from 90 crania of shrew specimens based on three craniodental views (dorsal, jaw, and lateral). Principal component analysis (PCA) and linear discriminant analysis (LDA) were applied to both GM and FDGM methods to classify the three shrew species. This study also compared four machine learning approaches (naïve Bayes, support vector machine, random forest, and generalised linear models) using predicted PC scores obtained from both methods (combination of all three craniodental views and individual views). The analyses favoured FDGM and the dorsal view was the best view for distinguishing the three species. Overall, the generalised linear models (GLM) was the most accurate (95.4% accuracy) among the four classification models.
WGCNA and multiple machine learning methods identified SV2C and DENR as novel biomarkers for Parkinson's disease
Authors: Wu Jiecong , Wu Wenqi , Jiang Ping , Xu Yuhao , Yu Ming 
Published: October 2023

Abstract
In the past servel years, the incidence of Parkinson's disease has continued to rise globally, placing a heavy burden on patients and society. Nevertheless, diagnostic and therapeutic tools for the disease have not been completely optimized. Consequently, the current study concentrated on determining promising biomarkers in the brain tissues of Parkinson's disease individuals and performing functional analyses. In the present work, we aimed to determine the hub genes providing Diagnostic and therapeutic targets for Parkinson's disease. GSE8397, GSE20292, GSE20163, GSE20164, and GSE49036 from the Gene Expression Omnibus (GEO) database were analyzed. Weighted gene co-expression network analysis (WGCNA) and DEGs determined 42 intersecting genes. Subsequently, the genes SV2C and DENR were identified as biomarkers of Parkinson's disease with machine learning. Finally, functional analysis suggested that SV2C may be involved in dopamine vesicle transport. Immune infiltration analysis revealed remarkable differences between SV2C for multiple immune cells. DENR and SV2C expression was demonstrated in PD cell models and clinical human samples.. In conclusion, The SV2C and DENR genes in brain tissue are viable biomarkers and therapeutic targets for Parkinson's disease which can facilitate diagnosis and treatment in clinical practice.
Hotspots and frontiers in PSMA research for prostate cancer: a bibliometric and visualization analysis over the past 20 years
Authors: Zhang Hanfei , Liu Xiao , Xie Hangyu , Li Lin 
Published: October 2023

Abstract
Background: Prostate-specific membrane antigen (PSMA)-targeted imaging and therapy have significantly changed the management of patients with prostate cancer (PCa) at different disease stages. This advancement has attracted the attention of scholars, leading to a prolific output of scholarly publications. This study comprehensively outlines the knowledge framework associated with PSMA-based diagnosis and treatment of PCa through the application of bibliometric analysis, and discusses the potential research trends and foci.
Methods: Articles and reviews related to PSMA for prostate cancer from 2003 to 2022 were retrieved from Web of Science Core Collection. VOSviewer, Citespace, and R-bibliometrix were primarily employed to execute and visually represent co-authorship, co-citation, and co-occurrence analysis of countries, institutions, authors, references and keywords in this field.
Results: A total of 3830 papers were included. The papers on the field of PSMA-based PCa therapy and imaging had been continuously increased since 2003, but the rate has slowed from 2020. The United States made the largest contribution in this field, in terms of publications 997 (26.03%), H-index (110) and total citations (53,167 times). We identified the most productive institution were Technical University of Munich, and Australian institutions had become very active in recent years. Journal of Nuclear Medicine was the most prominent journal in this field. Professors Matthias Eiber and Martin G Pomper made great achievements, while Ali Afshar-Oromieh was the most co-cited author. According to the result of keywords and topics analysis, “ga-68 labeled psma ligand”, “radiation dosimetry” and “HBED-CC” were major research areas in the near future, while "Extended pelvic lymph node dissection" was considered to be the future research foci.
Conclusions: The field of psma-based PCa therapy and imaging is in the stage of vigorous development and has a bright prospect. The United States and Germany have achieved outstanding results in this area, while Australia has recently developed rapidly. It is foreseeable that more research foci will be lied in the early detection of pelvic lymph nodes and the multimodal imaging-guided surgery.
Short Communications: Assessing morphological preservation of gastrointestinal helminths in ethanol versus formalin
Authors: Hass Joelle , Henriquez Megan C. , Churcher Jessica , Hamou Hadjira , Morales Suheidy Romero , Melin Amanda D. 
Published: October 2023

Abstract
The accurate identification of gastrointestinal parasites from animal feces is vital for understanding many aspects of host-parasite interactions. Copromicroscopic parasite identification is a longstanding, frequently used, and cost-effective method, but its efficacy depends on the storage medium of the samples. In this study we compare the preservation of parasite larvae and eggs in ethanol versus formalin-preserved fecal samples collected from a wild population of Costa Rican capuchin monkeys (Cebus imitator). Samples were collected, halved, and stored in either 10% formalin or 96% ethanol at ambient temperature, then screened for the presence of parasites using a modified Wisconsin sedimentation technique and microscopy. Parasites were morphologically identified and graded on their preservation using a rubric we developed. We identified more parasitic morphotypes in samples preserved in formalin but found no difference in counts of parasites per fecal gram between mediums. The two most prevalent genera of parasites, Filariopsis and Strongyloides, were found in similar numbers in the different mediums and while Filariopsis larvae were better preserved in formalin, strongyle eggs showed no difference in preservation between mediums. Parasites preserved in ethanol and formalin were morphologically identifiable in samples stored up to 2 years, highlighting the suitability of both mediums in morphological parasite identification. While formalin-preserved samples may not be suitable for subsequent genetic analyses, ethanol-preserved samples can be studied further using molecular methods. Future studies may build on our current understanding of parasite ecology by pairing morphological and molecular methods to explore taxonomic diversity and deepen our understanding of host-parasite relationships.