bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioconversion of Glucose-Rich Lignocellulosic Wood Hydrolysates to 3-Hydroxypropionic Acid and Succinic Acid using EngineeredSaccharomyces cerevisiae
Authors: Bottoms Scott , Mürk Christina , Peng Huadong , Ledesma-Amaro Rodrigo , Loog Mart 
Published: November 2023

AbstractBackground3-hydroxypropionic acid (3-HP) and succinic acid (SA) were announced as two of the top twelve value-added platform chemicals from biomass out of a group of over 300 potential compounds that could be made from biomass in a government report in 2004 (Werpy and Petersen, 2004) and in an updated report in 2010 (Bozell and Petersen, 2010). The screening criteria used in the report classified 3-HP and SA as direct petroleum replacement building block chemicals. 3-HP is a precursor to several high-value compounds, such as acrylic acid, 1,3-propanediol, acrylamide, and methyl acrylates, that ultimately end up in products such as fibers, contact lenses, diapers, fabric coatings, and other super absorbent polymers (SAPs). SA is a high-value platform chemical used in polyester production and a precursor for nylon and other bioplastics. Additionally, these reports identified pathways to building block compounds from sugars. Yeast fermentations were identified in these reports as a preferred potential pathway to 3-HP and SA production from sugars because of yeasts’ natural low pH tolerance.ResultsThe laboratory strainSaccharomyces cerevisiaeBY4741 was engineered to produce either 3-HP or SA. These yeasts can convert fermentable sugars from glucose-rich lignocellulosic hardwood feedstocks into organic acid products such as 3-HP and SA under low pH conditions using exponential fed-batch cultivation strategies. Glucose-rich wood sugars provided a better growth environment for the engineered yeast strains, increasing production titers by 6.1 and 6.5 times for SA and 3-HP, respectively.ConclusionsThis study shows the potential of locally produced glucose-rich wood sugars to increase the production of platform chemicals necessary in the production of biobased polymers by engineered yeast cell factories.
Benchmarking sample pooling for epigenomics of natural populations
Authors: Daniels Ryan J. , Meyer Britta S. , Giulio Marco , Signorini Silvia G. , Riccardi Nicoletta , Torre Camilla Della , Weber Alexandra A.-T. 
Published: November 2023

AbstractInterest in the role of DNA methylation (DNAm) has grown in ecological and evolutionary research of natural populations. While researchers are typically interested in comparing population-level variation, individual sequencing is the current standard. Natural populations have low effect sizes and thus need large sample sizes to detect differences. The cost of sequencing the necessary samples can be prohibitive in DNAm work. Pooling DNA before library preparation is a powerful tool to reduce costs but no recommendations exist for DNAm pooling in ecology-epigenetics research. We test if pooled and individual libraries provide similar global and region-specific DNA methylation signals in a natural system of response to pollution. We generated whole-epigenome data for two freshwater invasive molluscs (Corbicula fluminaandDreissena polymorpha) collected from a polluted and unpolluted locality, Lake Maggiore, Italy. Our results support that pooling effectively captures the same genome-wide and global treatment-level signals as individual libraries but we note that pooled libraries yielded orders of magnitude more input data and differentially-methylated regions (DMRs) detected compared with individual libraries. We estimated greatly lower power for regions from individual libraries compared with pooled libraries. The post-hoc process of computationally pooling data from individual libraries produced results comparable to pooled libraries in volumes but had discrepancies between DMRs. We discuss the possible causes for the discrepancies and put our results in the context of the benefits and drawbacks of sample pooling for epigenomics of natural populations.
StayGold Photostability under Different Illumination Modes
Authors: Hirano Masahiko , Yonemaru Yasuo , Shimozono Satoshi , Sugiyama Mayu , Ando Ryoko , Okada Yasushi , Fujiwara Takahiro , Miyawaki Atsushi 
Published: November 2023

AbstractStayGold is a bright fluorescent protein (FP) that is over one order of magnitude more photostable than any of the currently available FPs across the full range of illumination intensities used in widefield microscopy and structured illumination microscopy, the latter of which is a widefield illumination-based technique. To compare the photostability of StayGold under other illumination modes with that of three other green-emitting FPs, namely EGFP, mClover3, and mNeonGreen, we expressed all four FPs as fusions to histone 2B in HeLa cells. Unlike the case of widefield microscopy, the photobleaching behavior of these FPs in laser scanning confocal microscopy (LSCM) is complicated. The outstanding photostability of StayGold observed in multi-beam LSCM was variably attenuated in single-beam LSCM, which produces intermittent and instantaneously strong illumination. We systematically examined the effects of different single-beam LSCM beam-scanning patterns on the photostability of the FPs. This study offers relevant guidelines for researchers who aim to achieve sustainable imaging by resolving problems related to FP photostability.
An experimental target-based platform in yeast for screeningPlasmodium vivaxdeoxyhypusine synthase inhibitors
Authors: Silva Suélen Fernandes , Klippel Angélica Hollunder , Sigurdardóttir Sunniva , Mahdizadeh Sayyed Jalil , Tiukova Ievgeniia , Bourgard Catarina , Monteiro do Amaral Prado Heloísa , de Araujo Renan Vinicius , Bilsland Elizabeth , King Ross D. , Massirer Katlin Brauer , Eriksson Leif A. , Bengtson Mário Henrique , Sunnerhagen Per , Zanelli Cleslei Fernando 
Published: November 2023

ABSTRACTThe enzyme deoxyhypusine synthase (DHS) catalyzes the first step in the post-translational modification of the eukaryotic translation factor 5A (eIF5A). This is the only protein known to contain the amino acid hypusine, which results from this modification. Both eIF5A and DHS are essential for cell viability in eukaryotes, and inhibiting DHS can be a promising strategy for the development of new therapeutic alternatives. The human and parasitic orthologous proteins are different enough to render selective targeting against infectious diseases; however, no DHS inhibitor selective for the parasite ortholog has previously been reported. Here, we established a yeast surrogate genetics platform to identify inhibitors of DHS fromPlasmodium vivax,one of the major causative agents of malaria. We constructed genetically modifiedSaccharomyces cerevisiaestrains expressing DHS genes fromHomo sapiens(HsDHS) orP. vivax(PvDHS) in place of the endogenous DHS gene fromS. cerevisiae. This new strain background was ∼60-fold more sensitive to an inhibitor of human DHS than the one previously used. Initially, a virtual screen using datasets from the ChEMBL-NTD database was performed. Candidate ligands were tested in growth assays using the newly generated yeast strains expressing heterologous DHS genes. Among these, two showed promise by preferentially reducing the growth of the PvDHS-expressing strain. Further, in a robotized assay, we screened 400 compounds from the Pathogen Box library using the sameS. cerevisiaestrains, and one compound preferentially reduced the growth of the PvDHS-expressing yeast strain. Western blot revealed that these compounds significantly reduced eIF5A hypusination in yeast. Our study demonstrates that this yeast-based platform is suitable for identifying and verifying candidate small molecule DHS inhibitors, selective for the parasite over the human ortholog.
The complexities of embracing mechatronics in the Nigerian construction industry
Authors: Oke Ayodeji Emmanuel , Aliu John , Oni Samuel Bankole , Ilesanmi Oluwadamilare Olamide 
Published: November 2023

Purpose
The purpose of this study is to investigate the obstacles to mechatronics adoption in the construction industry from a Nigerian perspective. It aims to fill the knowledge gap by focusing on the specific challenges faced in developing countries, considering the unique contexts and constraints of the Nigerian construction industry.

Design/methodology/approach
The study used a comprehensive literature review to identify 26 obstacles to mechatronics adoption. These obstacles were used to develop a well-structured questionnaire, which was then distributed to construction professionals using Google Forms through purposive and snowball sampling techniques. The rankings obtained from the questionnaire responses were analyzed to determine the most significant obstacles.

Findings
The study revealed the top five most significant obstacles to mechatronics adoption in the Nigerian construction industry. These obstacles include high costs of operation and maintenance, resistance to adopting new technologies, a lack of standardized protocols, insufficient maintenance capabilities and a lack of government support. Factor analysis revealed five clusters of obstacles: technological-related factors, economic-related factors, capability-related factors, government-related factors and awareness-related factors.

Practical implications
Findings from this study have the potential to inform decision-making, drive policy changes and guide future research efforts aimed at promoting the widespread adoption of mechatronics technologies, ultimately leading to the transformation and improvement of the construction industry as a whole.

Originality/value
This study contributes to the field of mechatronics adoption in the construction industry by addressing the gap in research specific to developing countries such as Nigeria. By identifying and analyzing the obstacles from a Nigerian perspective, the study offers unique insights and original findings.
Workplace information literacy in context: scale development and validation
Authors: Aslam Maria , Batool Syeda Hina , Ameen Kanwal 
Published: November 2023

Purpose
The study aims to develop a validated scale to measure the workplace information literacy (WIL) skills of Quality Enhancement Cell’s staff based on the i-skills model.

Design/methodology/approach
To develop scale, a multi-stage scale development procedure was adopted. The comprehensive WIL scale was developed and validated in four phases. The first phase involved reviewing literature, content and face validity through experts. In the later stages, through survey method, the construct validity was tested with the application of the exploratory factor analysis and principal component analysis. The scale reliability further calculated through Cronbach’s alpha internal reliability coefficient. The SPSS was used to test and validate the designed scale.

Findings
A multi-dimensional, reliable 62-item scale classified into eight factors was developed. Results indicate that the designed scale achieved internal, face, content, construct validity and reliability. The refined scale has the potential to be used in similar context, however, can be modified for diverse contexts and situations.

Originality/value
To the best of authors’ knowledge, this is the first validated scale of WIL based on the i-skills model. Looking at the prior developed scales, this tool is unique in nature to focus on the skills of administrative staff.
To stay or go? Understanding ambivalence toward moving on from supportive housing among supportive housing residents in a Moving On initiative
Authors: Livingstone Kimberly , Tiderington Emmy 
Published: November 2023

Purpose
Moving On initiatives (MOIs) provide opportunities for permanent supportive housing (PSH) residents to exit PSH services for mainstream housing. This study aims to better understand the factors that influence residents’ decisions on whether to leave or stay in these services.

Design/methodology/approach
This study examines ambivalence toward leaving PSH using interviews with 40 MOI participants. Data were analyzed using framework analysis.

Findings
“Pull factors” for staying in PSH included secure benefits, partial autonomy and being comfortable enough. Participants viewed PSH as their cheapest option and a protective force. They also described PSH as “semi-independence” and expressed concern with a lack of privacy, free movement and unwanted supervision.

Originality/value
To the best of the authors’ knowledge, this is the first study to consider residents’ motivations for staying in PSH when given the opportunity to leave. Implications for PSH service delivery and implementation of MOIs are discussed.
Individual factors impacting tourist satisfaction and revisit intention in slow tourism cities: an extended model
Authors: Baghirov Fakhri , Bozbay Zehra , Zhang Ye 
Published: November 2023

Purpose
Postpandemic efforts to rebuild have steered the global economy toward a more sustainable trajectory. It is imperative to acknowledge the pressing need for further enhancements in the sustainable development of the tourism industry. This study aims to examine the influence of personal factors, including environmental concern, cultural interest, travel lifestyle and involvement, on tourist satisfaction and revisit intention, using the theory of planned behavior (TPB) as its framework.

Design/methodology/approach
The data was gathered through surveys conducted in three of Türkiye’s most famous slow travel destinations: Seferihisar, Gökçeada and Akyaka. The analysis was carried out using SPSS and SmartPLS software, with subsequent structural model testing.

Findings
This study presents an extended model that incorporates four individual factors, tourist satisfaction, TPB and revisit intention. All hypotheses have been rigorously tested, and the model accounts for 60.4% of the variance in revisit intention. The findings are comprehensively discussed in this article, supported by relevant theoretical frameworks.

Research limitations/implications
Future research avenues could delve into the evolution of slow tourism in both developed and developing countries, assess disparities in revisit intentions between slow tourism and mass tourism destinations and investigate the prospects of sustainable tourism development in the postpandemic era.

Originality/value
The authors use the TPB to examine individual factors, tourist satisfaction and revisit intentions, aiming to build an extended model to gain a deeper understanding of the slow tourist decision-making process.
Regional heterogeneity in undernourishment: the case of Nepal
Authors: Poudel Dikshit , Gopinath Munisamy 
Published: November 2023

PurposeEnsuring adequate access to food to all has remained a major challenge of the 21st century. To aid the effort of hunger alleviation, many studies have quantified the prevalence of undernourishment (PoU), mostly at the national level. There has been limited attention to understanding the intra-country variation in undernourishment estimates.Design/methodology/approachUnlike past studies, this study tracks the substantial regional heterogeneity in the undernourishment status within Nepal. Employing Food and Agriculture Organization's methodology and Nepal Living Standards Survey data from 1995, 2003 and 2011, household energy consumption and requirements are computed to determine regional undernourishment.FindingsNepal's PoU declined between 1995 and 2003 but increased after 2003 affecting approximately 7.6 million Nepalese in 2011. The Terai domain – Provinces 2 and 5 – are found to be the most vulnerable to undernourishment concerns likely because of economic and natural shocks. Province 4 achieved higher progress in alleviating undernourishment during 2003, but its PoU doubled in 2011 (as in Province 6).Research limitations/implicationsBy examining where and how many are undernourished within Nepal, this study has provided a more accurate picture of the PoU for better-targeting assistance to improve the livelihood of its citizens.Originality/valuePast studies indicate substantial variation in food access and PoU within Nepal. While they provide some answers to “where and how many” questions for some districts, provinces, belts and urban/rural domains at the cross-sectional level (or for selected locales), few have examined intra-Nepal heterogeneity, especially over time. Therefore, this study explores where and how many within Nepal have been undernourished during the past three decades.
Spain is different! An analysis of the promotion strategies in the tourism industry in Spain
Authors: Blázquez-Resino Juan José , Martínez-Ruiz María Pilar , Muro Rodríguez Ana Isabel 
Published: November 2023

Purpose
Given the great tourist attractiveness of Spain at international level, tourism has become one of the main sources of income and employment, as well as a basic pillar of the Spanish economy. With these ideas in mind, this paper aims to study how the different promotion strategies implemented in the industry have influenced the evolution of tourism in Spain since the early 20th century.

Design/methodology/approach
The research is a general review, providing a historical examination of the diverse promotion strategies deployed in the tourism industry in Spain over the past decades. It focuses on the descriptive approach of these strategies and their implications throughout the 20th century and the beginning of the 21st century.

Findings
The findings reveal a shift in recent years from strategies focused on Marketing 1.0 to strategies that, apart from being centered on consumer values (therefore, Marketing 3.0), are beginning to rely to a greater extent on information and communication technologies (ICT) and sustainability, more in line with the more recent Marketing 4.0 and even Marketing 5.0.

Social implications
This work has many implications for the management of public and private operators in the industry, including the need to incorporate the latest marketing trends – most notably the advances in ICT and sustainability.

Originality/value
The study offers an in-depth understanding of how marketing strategies have been used in the tourism sector in Spain from the end of the 19th century to the present day, which is highly original compared to previous studies.
A mixed-methods process evaluation of an integrated care system's population health management system to reduce health inequalities in COVID-19 vaccination uptake
Authors: Watson Georgia , Moore Cassie , Aspinal Fiona , Hutchings Andrew , Raine Rosalind , Sheringham Jessica 
Published: November 2023

PurposeMany countries have a renewed focus on health inequalities since COVID-19. In England, integrated care systems (ICSs), formed in 2022 to promote integration, are required to reduce health inequalities. Integration is supported by population health management (PHM) which links data across health and care organisations to inform service delivery. It is not well-understood how PHM can help ICSs reduce health inequalities. This paper describes development of a programme theory to advance this understanding.Design/methodology/approachThis study was conducted as a mixed-methods process evaluation in a local ICS using PHM. The study used Framework to analyse interviews with health and care professionals about a PHM tool, the COVID-19 vaccination uptake Dashboard. Quantitative data on staff Dashboard usage were analysed descriptively. To develop a wider programme theory, local findings were discussed with national PHM stakeholders.FindingsICS staff used PHM in heterogeneous ways to influence programme delivery and reduce inequalities in vaccine uptake. PHM data was most influential where it highlighted action was needed for “targetable” populations. PHM is more likely to influence decisions on reducing inequalities where data are trusted and valued, data platforms are underpinned by positive inter-organisational relationships and where the health inequality is a shared priority.Originality/valueThe COVID-19 pandemic accelerated a shift toward use of digital health platforms and integrated working across ICSs. This paper used an evaluation of integrated data to reduce inequalities in COVID-19 vaccine delivery to propose a novel programme theory for how integrated data can support ICS staff to tackle health inequalities.
Trade books' historical representation of Margaret Sanger
Authors: Bickford John H. 
Published: November 2023

PurposeThis content analysis examines the historical representation of Margaret Sanger within trade books. From the framework of the historiography, this paper unpacks how common curricular resources depict an American icon with a complicated past.Design/methodology/approachIn this paper, the author conducted a content analysis of biographies and expository compilations featuring Sanger. The entire data pool were sampled and analyzed.FindingsThe trade books, particularly the biographies, historically represented Sanger in most categories. Sanger's international direct action and eugenics were two misrepresented areas. Expository compilations, with more limited space than biographies, contained more omissions and minimized or vague depictions of key areas. Findings did not appear dependent upon date of publication.Originality/valueThis study explores an icon of America's free speech battles and birth control rights at a time when culture wars are shaping current events. No researchers have previously explored Sanger's historical representation within trade books.
NR0B1 augments sorafenib resistance in hepatocellular carcinoma through promoting autophagy and inhibiting apoptosis
Authors: Tan Xiao lan , Wang Zhaokun , Liao Shunyao , Yi Ming , Tao Dachang , Zhang Xinyue , Leng Xiangyou , Shi Jiaying , Xie Shengyu , Yang Yuan , Liu Yun qiang 
Published: November 2023

AbstractNR0B1 is frequently activated in hepatocellular carcinoma (HCC). However, the role of NR0B1 is controversial in HCC. In this study, we observed that NR0B1 was an independent poor prognostic factor, negatively correlated with the overall survival of HCC and the relapse‐free survival of patients treated with sorafenib. Meanwhile, NR0B1 promoted the proliferation, migration, and invasion of HCC cells, inhibited sorafenib‐induced apoptosis, and elevated the IC50 of sorafenib in HCC cells. NR0B1 was further displayed to increase sorafenib‐induced autophagic vesicles and activate Beclin1/LC3‐II‐dependent autophagy pathway. Finally, NR0B1 was revealed to transcriptionally suppress GSK3β that restrains AMPK/mTOR‐driven autophagy and increases BAX‐mediated apoptosis. Collectively, our study uncovered that the ectopic expression of NR0B1 augmented sorafenib‐resistance in HCC cells by activating autophagy and inhibiting apoptosis. Our findings supported that NR0B1 was a detrimental factor for HCC prognosis.