bluuebunny/crossref_metadata_2025_split huggingface dataset:
Redox-based ion-gating reservoir consisting of (104) oriented LiCoO2 film, assisted by physical masking
Authors: Shibata Kaoru , Nishioka Daiki , Namiki Wataru , Tsuchiya Takashi , Higuchi Tohru , Terabe Kazuya 
Published: November 2023

AbstractReservoir computing (RC) is a machine learning framework suitable for processing time series data, and is a computationally inexpensive and fast learning model. A physical reservoir is a hardware implementation of RC using a physical system, which is expected to become the social infrastructure of a data society that needs to process vast amounts of information. Ion-gating reservoirs (IGR) are compact and suitable for integration with various physical reservoirs, but the prediction accuracy and operating speed of redox-IGRs using WO3 as the channel are not sufficient due to irreversible Li+ trapping in the WO3 matrix during operation. Here, in order to enhance the computation performance of redox-IGRs, we developed a redox-based IGR using a (104) oriented LiCoO2 thin film with high electronic and ionic conductivity as a trap-free channel material. The subject IGR utilizes resistance change that is due to a redox reaction (LiCoO2 ⟺ Li1−xCoO2 + xLi+ + xe−) with the insertion and desertion of Li+. The prediction error in the subject IGR was reduced by 72% and the operation speed was increased by 4 times compared to the previously reported WO3, which changes are due to the nonlinear and reversible electrical response of LiCoO2 and the high dimensionality enhanced by a newly developed physical masking technique. This study has demonstrated the possibility of developing high-performance IGRs by utilizing materials with stronger nonlinearity and by increasing output dimensionality.
Distance and destination of retail meat alter multidrug resistant contamination in the United States food system
Authors: Innes Gabriel K. , Patton Andrew N. , Nachman Keeve E. , Casey Joan A. , Stapleton G. Sean , Abraham Alison G. , Price Lance B. , Tartof Sara Y. , Davis Meghan F. 
Published: November 2023

AbstractAntibiotic-resistant infections are a global concern, especially those caused by multidrug-resistant (MDR) bacteria, defined as those resistant to more than three drug classes. The animal agriculture industry contributes to the antimicrobial resistant foodborne illness burden via contaminated retail meat. In the United States, retail meat is shipped across the country. Therefore, understanding geospatial factors that influence MDR bacterial contamination is vital to protect consumers and inform interventions. Using data available from the United States Food and Drug Administration’s National Antimicrobial Resistance Monitoring System (NARMS), we describe retail meat shipping distances using processor and retailer locations and investigated this distance as a risk factor for MDR bacteria meat contamination using log-binomial regression. Meat samples collected during 2012–2014 totaled 11,243, of which 4791 (42.61%) were contaminated with bacteria and 835 (17.43%) of those bacteria were MDR. All examined geospatial factors were associated with MDR bacteria meat contamination. After adjustment for year and meat type, we found higher prevalence of MDR contamination among meat processed in the south (relative adjusted prevalence ratio [aPR] 1.35; 95% CI 1.06–1.73 when compared to the next-highest region), sold in Maryland (aPR 1.12; 95% CI 0.95–1.32 when compared to the next-highest state), and shipped from 194 to 469 miles (aPR 1.59; 95% CI 1.31–1.94 when compared to meats that traveled < 194 miles). However, sensitivity analyses revealed that New York sold the meat with the highest prevalence of MDR Salmonella contamination (4.84%). In this secondary analysis of NARMS data, both geographic location where products were sold and the shipping distance were associated with microbial contamination on retail meat.
Metabolomics of early blight (Alternaria solani) susceptible tomato (Solanum lycopersicum) unfolds key biomarker metabolites and involved metabolic pathways
Authors: Singh Dhananjaya Pratap , Maurya Sudarshan , Yerasu Suresh Reddy , Bisen Mansi Singh , Farag Mohamed A. , Prabha Ratna , Shukla Renu , Chaturvedi Krishna Kumar , Farooqi Md. Samir , Srivastava Sudhir , Rai Anil , Sarma Birinchi Kumar , Rai Nagendra , Behera Tusar Kanti 
Published: November 2023

AbstractTomato (Solanum lycopersicum) is among the most important commercial horticultural crops worldwide. The crop quality and production is largely hampered due to the fungal pathogen Alternaria solani causing necrotrophic foliage early blight disease. Crop plants usually respond to the biotic challenges with altered metabolic composition and physiological perturbations. We have deciphered altered metabolite composition, modulated metabolic pathways and identified metabolite biomarkers in A. solani-challenged susceptible tomato variety Kashi Aman using Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics. Alteration in the metabolite feature composition of pathogen-challenged (m/z 9405) and non-challenged (m/z 9667) plant leaves including 8487 infection-exclusive and 8742 non-infection exclusive features was observed. Functional annotation revealed putatively annotated metabolites and pathway mapping indicated their enrichment in metabolic pathways, biosynthesis of secondary metabolites, ubiquinone and terpenoid-quinones, brassinosteroids, steroids, terpenoids, phenylpropanoids, carotenoids, oxy/sphingolipids and metabolism of biotin and porphyrin. PCA, multivariate PLS-DA and OPLS-DA analysis showed sample discrimination. Significantly up regulated 481 and down regulated 548 metabolite features were identified based on the fold change (threshold ≥ 2.0). OPLS-DA model based on variable importance in projection (VIP scores) and FC threshold (> 2.0) revealed 41 up regulated discriminant metabolite features annotated as sphingosine, fecosterol, melatonin, serotonin, glucose 6-phosphate, zeatin, dihydrozeatin and zeatin-β-d-glucoside. Similarly, 23 down regulated discriminant metabolites included histidinol, 4-aminobutyraldehyde, propanoate, tyramine and linalool. Melatonin and serotonin in the leaves were the two indoleamines being reported for the first time in tomato in response to the early blight pathogen. Receiver operating characteristic (ROC)-based biomarker analysis identified apigenin-7-glucoside, uridine, adenosyl-homocysteine, cGMP, tyrosine, pantothenic acid, riboflavin (as up regulated) and adenosine, homocyctine and azmaline (as down regulated) biomarkers. These results could aid in the development of metabolite-quantitative trait loci (mQTL). Furthermore, stress-induced biosynthetic pathways may be the potential targets for modifications through breeding programs or genetic engineering for improving crop performance in the fields.
Species-specific gill’s microbiome of eight crab species with different breathing adaptations
Authors: Bacci Giovanni , Meriggi Niccolò , Cheng Christine L. Y. , Ng Ka Hei , Iannucci Alessio , Mengoni Alessio , Cavalieri Duccio , Cannicci Stefano , Fratini Sara 
Published: November 2023

AbstractTransitions to physically different environments, such as the water-to-land transition, proved to be the main drivers of relevant evolutionary events. Brachyuran crabs evolved remarkable morphological, behavioral, and physiological adaptations to terrestrial life. Terrestrial species evolved new respiratory structures devoted to replace or support the gills, a multifunctional organ devoted to gas exchanges, ion-regulation and nitrogen excretion. It was hypothesized that microorganisms associated with respiratory apparatus could have facilitated the processes of osmoregulation, respiration, and elimination of metabolites along this evolutionary transition. To test if crab species with different breathing adaptations may host similar microbial communities on their gills, we performed a comparative targeted-metagenomic analysis, selecting two marine and six terrestrial crabs belonging to different families and characterised by different breathing adaptations. We analysed anterior and posterior gills separately according to their different and specific roles. Regardless of their terrestrial or marine adaptations, microbial assemblages were strongly species-specific indicating a non-random association between the host and its microbiome. Significant differences were found in only two terrestrial species when considering posterior vs. anterior gills, without any association with species-specific respiratory adaptations. Our results suggest that all the selected species are strongly adapted to the ecological niche and specific micro-habitat they colonise.
Comparative evaluation of 3D-printed and conventional implants in vivo: a quantitative microcomputed tomographic and histomorphometric analysis
Authors: Suh Hyemee , Lee Dongseob , Lee Jungwon , Seol Yang-Jo , Lee Yong-Moo , Koo Ki-Tae 
Published: November 2023

AbstractIn recent years, 3D-printing technology to fabricate dental implants has garnered widespread attention due to its patient-specific customizability and cost-effectiveness. This preclinical animal study analyzed the radiographic and histomorphometric outcomes of 3D-printed implants (3DIs) placed immediately after extraction and compared them to conventional implants (CIs). 3DIs and CIs of the same dimensions placed immediately were analyzed at 2, 6, and 12 weeks. The micro-computed tomography (micro-CT) analysis revealed statistically significant differences at 2 weeks in favor of 3DIs over the CIs in terms of bone volume/tissue volume (BV/TV), bone surface/bone volume (BS/BV), trabecular bone pattern factor (Tb.Pf), and structure model index (SMI). At 2 weeks, the mean bone-to-implant contact (BIC) of the 3DIs was greater than that of the CIs; the mean bone area fraction occupancy (BAFO) and the number of Haversian canals of the 3DIs showed no statistically significant differences compared to CIs at 2 weeks. At 6 and 12 weeks, there were no statistically significant differences between the 3DIs and CIs in any parameters. Within limitations, in the early stage of extraction socket healing, the 3DIs demonstrated a higher BIC than the CIs, presenting that 3DIs may be a potential option for immediate placement to enhance osseointegration.
C1QA and COMP: plasma-based biomarkers for early diagnosis of pancreatic neuroendocrine tumors
Authors: Gorai Priya Kumari , Bharti Prahalad Singh , Kumar Shashi , Rajacharya Girish H. , Bandyopadhyay Sabyasachi , Pal Sujoy , Dhingra Renu , Kumar Rakesh , Nikolajeff Fredrik , Kumar Saroj , Rani Neerja 
Published: November 2023

AbstractPancreatic Neuroendocrine tumors (PanNET) are challenging to diagnose and often detected at advanced stages due to a lack of specific and sensitive biomarkers. This study utilized proteomics as a valuable approach for cancer biomarker discovery; therefore, mass spectrometry-based proteomic profiling was conducted on plasma samples from 12 subjects (3 controls; 5 Grade I, 4 Grade II PanNET patients) to identify potential proteins capable of effectively distinguishing PanNET from healthy controls. Data are available via ProteomeXchange with the identifier PXD045045. 13.2% of proteins were uniquely identified in PanNET, while 60% were commonly expressed in PanNET and controls. 17 proteins exhibiting significant differential expression between PanNET and controls were identified with downstream analysis. Further, 5 proteins (C1QA, COMP, HSP90B1, ITGA2B, and FN1) were selected by pathway analysis and were validated using Western blot analysis. Significant downregulation of C1QA (p = 0.001: within groups, 0.03: control vs. grade I, 0.0013: grade I vs. grade II) and COMP (p = 0.011: within groups, 0.019: control vs grade I) were observed in PanNET Grade I & II than in controls. Subsequently, ELISA on 38 samples revealed significant downregulation of C1QA and COMP with increasing disease severity. This study shows the potential of C1QA and COMP in the early detection of PanNET, highlighting their role in the search for early-stage (Grade-I and Grade-II) diagnostic markers and therapeutic targets for PanNET.
An arrayed CRISPR knockout screen identifies genetic regulators of GLUT1 expression
Authors: Shi Yajuan , Katdare Ketaki A. , Kim Hyosung , Rosch Jonah C. , Neal Emma H. , Vafaie-Partin Sidney , Bauer Joshua A. , Lippmann Ethan S. 
Published: November 2023

AbstractGlucose, a primary fuel source under homeostatic conditions, is transported into cells by membrane transporters such as glucose transporter 1 (GLUT1). Due to its essential role in maintaining energy homeostasis, dysregulation of GLUT1 expression and function can adversely affect many physiological processes in the body. This has implications in a wide range of disorders such as Alzheimer’s disease (AD) and several types of cancers. However, the regulatory pathways that govern GLUT1 expression, which may be altered in these diseases, are poorly characterized. To gain insight into GLUT1 regulation, we performed an arrayed CRISPR knockout screen using Caco-2 cells as a model cell line. Using an automated high content immunostaining approach to quantify GLUT1 expression, we identified more than 300 genes whose removal led to GLUT1 downregulation. Many of these genes were enriched along signaling pathways associated with G-protein coupled receptors, particularly the rhodopsin-like family. Secondary hit validation confirmed that removal of select genes, or modulation of the activity of a corresponding protein, yielded changes in GLUT1 expression. Overall, this work provides a resource and framework for understanding GLUT1 regulation in health and disease.
Enhanced thermoelectric properties of self-assembling ZnO nanowire networks encapsulated in nonconductive polymers
Authors: Volkova Margarita , Sondors Raitis , Bugovecka Lasma , Kons Artis , Avotina Liga , Andzane Jana 
Published: November 2023

AbstractThe near-room temperature thermoelectric properties of self-assembling ZnO nanowire networks before and after encapsulation in nonconductive polymers are studied. ZnO nanowire networks were synthesized via a two-step fabrication technique involving the deposition of metallic Zn networks by thermal evaporation, followed by thermal oxidation. Synthesized ZnO nanowire networks were encapsulated in polyvinyl alcohol (PVA) or commercially available epoxy adhesive. Comparison of electrical resistance and Seebeck coefficient of the ZnO nanowire networks before and after encapsulation showed a significant increase in the network's electrical conductivity accompanied by the increase of its Seebeck coefficient after the encapsulation. The thermoelectric power factor (PF) of the encapsulated ZnO nanowire networks exceeded the PF of bare ZnO networks by ~ 5 and ~ 185 times for PVA- and epoxy-encapsulated samples, respectively, reaching 0.85 μW m−1 K−2 and ZT ~ 3·10–6 at room temperature, which significantly exceeded the PF and ZT values for state-of-the-art non-conductive polymers based thermoelectric flexible films. Mechanisms underlying the improvement of the thermoelectrical properties of ZnO nanowire networks due to their encapsulation are discussed. In addition, encapsulated ZnO nanowire networks showed excellent stability during 100 repetitive bending cycles down to a 5 mm radius, which makes them perspective for the application in flexible thermoelectrics.
Insights into the recognition mechanism in the UBR box of UBR4 for its specific substrates
Authors: Jeong Da Eun , Lee Hye Seon , Ku Bonsu , Kim Cheol-Hee , Kim Seung Jun , Shin Ho-Chul 
Published: November 2023

AbstractThe N-end rule pathway is a proteolytic system involving the destabilization of N-terminal amino acids, known as N-degrons, which are recognized by N-recognins. Dysregulation of the N-end rule pathway results in the accumulation of undesired proteins, causing various diseases. The E3 ligases of the UBR subfamily recognize and degrade N-degrons through the ubiquitin-proteasome system. Herein, we investigated UBR4, which has a distinct mechanism for recognizing type-2 N-degrons. Structural analysis revealed that the UBR box of UBR4 differs from other UBR boxes in the N-degron binding sites. It recognizes type-2 N-terminal amino acids containing an aromatic ring and type-1 N-terminal arginine through two phenylalanines on its hydrophobic surface. We also characterized the binding mechanism for the second ligand residue. This is the report on the structural basis underlying the recognition of type-2 N-degrons by the UBR box with implications for understanding the N-end rule pathway.
Intérêt de la kétamine dans le traitement des douleurs chroniques
Authors: Pickering Gisèle 
Published: November 2023

La kétamine est aujourd’hui fréquemment utilisée dans la prise en charge de la douleur chronique réfractaire aux traitements classiques. Selon les études, l’efficacité et les effets indésirables de la kétamine apparaissent variables, en lien avec des méthodologies et des modes d’administration hétérogènes, conduisant à des controverses sur son utilisation en clinique. Un besoin d’essais sur des cohortes plus nombreuses de patients mieux sélectionnés mais aussi d’études en « vraie vie » pour mieux quantifier son efficacité, raffiner les posologies de prescription et mieux comprendre ses effets indésirables à long terme, est régulièrement souligné dans la littérature. Toutefois, au cours des dernières années, plusieurs travaux ont permis d’avancer sur les recommandations d’utilisation. Ils soulignent en particulier la nécessité de prendre en compte à la fois les trajectoires diverses d’antalgie avec la kétamine, selon l’étiologie de la douleur, et les comorbidités, en particulier la dépression, afin d’optimiser la prise en charge des patients douloureux.
La sélection des patients en unités de soins palliatifs, une condition nécessaire du maintien du care
Authors: Thay Roger , Lefève Céline 
Published: November 2023

« Les humanités en santé : approches de terrain » sont coordonnées par Claire Crignon, professeure d’histoire et de philosophie des sciences à l’université de Lorraine, qui a créé le master « humanités biomédicales » à Sorbonne université.
Orthomolekulare Medizin beim Fibromyalgie-Syndrom
Authors: Böhm Udo 
Published: November 2023

ZusammenfassungDie Ätiologie des Fibromyalgie-Syndroms ist nur unklar definiert und konventionelle pharmakologische Therapiemaßnahmen sind oft unbefriedigend. Die orthomolekulare Medizin kann in Kombination mit Lebensstilmedizin und nichtmedikamentösen Maßnahmen eine zentrale Aufgabe in Sekundärprävention, komplementärer Therapie und Nachsorge des Fibromyalgie-Syndroms übernehmen. Sie kann gestörte Stoffwechselprozesse normalisieren und die wichtigsten somatischen und psychischen Symptome des Fibromyalgie-Syndroms verbessern, ohne dass im Gegensatz zur konventionellen Therapie mit schädlichen Nebenwirkungen zu rechnen ist. Der Artikel beschreibt das Fibromyalgie-Syndrom und stellt ein ganzheitlich orientiertes Konzept mit Mikronährstoffbeteiligung zur Diskussion.
A Rare Atypical Case of Asymptomatic and Spontaneous Intraneural Hematoma of Sural Nerve: A Case Report and Literature Review
Authors: Kang Shin Hyuk , Ahn Il Young , Kim Han Koo , Kim Woo Ju , Woo Soo Hyun , Kang Seung Hyun , Hong Soon Auck , Bae Tae Hui 
Published: November 2023

AbstractIntraneural hematoma is a rare disease that results in an impaired nerve function because of bleeding around the peripheral nerve, with only 20 cases reported. Trauma, neoplasm, and bleeding disorders are known factors for intraneural hematoma. However, here we report atypical features of asymptomatic and spontaneous intraneural hematoma which are difficult to diagnose.A 60-year-old woman visited our clinic with the complaint of a palpable mass on the right calf. She reported no medical history or trauma to the right calf and laboratory findings showed normal coagulopathy. Ultrasonography was performed, which indicated hematoma near saphenous vein and sural nerve or neurogenic tumor. We performed surgical exploration and intraneural hematoma was confirmed on sural nerve. Meticulous paraneuriotomy and evacuation was performed without nerve injury. Histological examination revealed intraneural hematoma with a vascular wall. No neurologic symptoms were observed.In literature review, we acknowledge that understanding anatomy of nerve, using ultrasonography as a diagnostic tool and surgical decompression is key for intraneural hematoma. Our case report may help establish the implications of diagnosis and treatment. Also, we suggested surgical treatment is necessary even in cases that do not present symptoms because neurological symptoms and associated symptoms may occur later.
Triggering competence may protect multiple minority members from hiring discrimination
Authors: Reese Jonathan , Santos Ana Sofia , Palma Tomás A. , Roberto Magda Sofia 
Published: November 2023

AbstractHiring managers may consider hundreds of applicants for one position, leading to rapid decisions based on minimal information which may discriminate against certain individuals. However, past research shows that individuals belonging to multiple minority groups may, in fact, benefit from their intersectional status in certain contexts. First, to identify possible types of prejudice, the Attitudes Toward Gay Men (ATG) and Attitudes Toward Brazilian Men (ATB) explicit measures were created and paired with implicit association tests (IATs). Whereas participants did not show signs of explicit negative attitudes toward outgroup members, they did exhibit implicit preferences toward ingroup members. Using another sample from the same sociocultural context, potential discrimination faced by straight or gay Brazilian applicants in Portugal was examined in high or low competence scenarios, drawing inspiration from the stereotype content model. Results indicated, compared to ingroup applicants, straight and gay outgroup members were perceived alike in competence and were offered statistically similar salaries when competence was triggered; in contrast, only straight outgroup members were perceived as less competent and were offered statistically lower salaries when competence was not triggered. Findings suggest that multiple minority status may protect certain individuals from hiring discrimination, particularly in contexts where competence is not assumed.
Exploring the performance of T-graphene for the anode of Mg-ion and Ca-ion batteries: A first-principles study
Authors: Obaidullah  , Habiba Umme , Piya Afiya Akter , Daula Shamim Siraj Ud 
Published: November 2023

Due to the growing need for high-capacity ion storage batteries, researchers are exploring new categories of electrode materials with superior electrochemical properties. In this investigation, a tetragonal symmetry of carbon named T-graphene has been taken into consideration as an anode material for Mg and Ca ion batteries for its superior electrical characteristics. To comprehend the adsorption behavior, charge transfer, and anodic properties of T-graphene, density functional theory has been employed. Initially, the most favorable adsorption sites for Mg/Ca insertion on the T-graphene have been identified and it found that bridge and octagonal hollow sites show high adsorption behavior with energy of about −1.99 and −4.86 eV, respectively. A significant amount of charge (Hirshfeld) about 0.24e and 0.79e transfer from Mg and Ca to T-graphene, respectively. When Mg/Ca is inserted, the electronic structure calculations demonstrate that T-graphene acts metallically. A very high specific capacity is found at about 1737.13 and 1985.30 mAh/g for Mg and Ca ion batteries, respectively. Moreover, the average open-circuit voltages for Mg are 0.65 V and 1.51 V for Ca. Therefore, it is assumed that T-graphene may be used as high-capacity anode material for Mg and Ca ion battery.