bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncovering Urinary Protein Biomarkers for Early Diagnosis and Evaluation of Hereditary Angioedema
Authors: Wu Jianqiang , Tang Xiaoyue , Zhou Nan , Wang Xue , Liu Peng , Zhang Zejian , Zhang Shuyang , Zhi Yuxiang 
Published: January 2024

Abstract
Purpose
Hereditary angioedema (HAE) is a rare and potentially life-threatening disease. Noninvasive and disease-specific biomarkers are needed for the early diagnosis and disease evaluation of HAE. We aimed to explore and identify urinary protein biomarkers of HAE in healthy controls (HCs) or disease-control patients.
Methods
Using data-independent acquisition (DIA) based urinary proteomics, differentially expressed proteins were identified between HAE and HC groups. Functional annotation of differentially expressed proteins was performed using Ingenuity pathway analysis. Then, the parallel reaction monitoring (PRM) targeted proteomics method was used in validation cohort 1 to validate some promising biomarker candidates. Furthermore, enzyme-linked immunosorbent assays (ELISA) were conducted in validation cohort 2 to verify pro-epidermal growth factor (EGF), C1 esterase inhibitor (C1-INH), and kininogen-1 (KNG1) levels.
Results
Among the 2562 urinary proteins identified, 269 showed differential expression between HAE and HC. The differentially expressed proteins were significantly enriched in phospholipase C signaling, coagulation system, acute phase response signaling, leukocyte extravasation signaling, and actin cytoskeleton signaling. In the biofunction analysis, these differential proteins were significantly enriched in leukocyte migration, adhesion of immune cells, endothelial cell development, permeability of the vascular system, and cell death of immune cells. Moreover, urinary clusterin level was significantly correlated with disease severity scores of HAE (R = -0.758, p < 0.01). A urinary biomarker panel (C1-INH, EGF, and KNG1) was validated in two independent clinical cohorts with area under the curve (AUC) values of 0.910 and 0.949 for HAE diagnosis.
Conclusions
This study describes the first application of a DIA-PRM-ELISA workflow to identify and validate noninvasive and HAE-specific biomarkers in urine. These findings will contribute to the pathogenesis research and biomarker discovery of HAE.
A Stochastic Model for the Immune Response to HIV/AIDS-TB Pathogenesis and Applications on Young Adults.
Authors: Shoko Claris , Molefe Wilford , Nadarajah Saralees 
Published: January 2024

Abstract
Background Coinfection of Mycobacterium tuberculosis (M.TB) and human immunodeficiency virus (HIV) accelerates immune deterioration. Approximately one in three people living with HIV dies due to TB. This is likely to hinder progress towards the achievement of the 2030 Sustainable Development Goal of ending the HIV and TB pandemic.Methods In this paper, we model HIV-TB interaction within the host using the deterministic approach. Further analysis of the progression of HIV in patients who were enrolled with TB and patients who developed TB during treatment is done using multi-state modelling. This study is done on HIV-TB co-infected young adults (15 to 34 years) from South Africa. HIV progression for this cohort is divided into 4 states (state 1: Undetectable viral load below 50 HIV RNA per mL; state 2: HIV RNA ranging from 50 to below 10 000 copies/mL; state 3: at least 10 000 HIV RNA copies/mL); and state 4: Death).Results Results from the analysis show that TB increases the odds of an unsuppressed viral load. This is quite notable for patients in state 2 where the log-linear effect of having TB at enrolment is approximately − 8.7 for the transition to state 1 and − 0.64 for the transition to state 3. At state 2 of HIV progression, the rate of virologic failure is also very high and most deaths are observed from this state.Conclusion This calls for the need to closely monitor HIV patients for any possibility of TB coinfection. This can be done by assigning treatment partners to all HIV patients.
On the determinants of the Fiscal Policy Response to COVID-19: Evidence from Robust Estimation and country outlier detection
Authors: Emmanouilidis Kyriakos , Golitsis Petros 
Published: January 2024

Abstract

This paper analyzes the determinants of fiscal policies during the Covid-19 crisis. Using the robust regression MM-estimator for 180 countries we have found that GDP per capita, quantitative easing, debt to GDP ratio, trade openness and total revenue had a positive and a statistically significant impact on the fiscal policy responses, while unemployment rate and current account balance had a negative one. Furthermore, we have shown that the proportion of the population aged 65 and above had a positive influence on the size of fiscal packages. In contrast, factors such as political stability, absence of violence, and political ideology had no discernible impact on the allocation of fiscal aid. The outcomes of our findings carry substantial implications and offer valuable insights for authorities and policymakers.
JEL No.: E62, E63, G01, G28
Role of sRNA molecules in the rye–leaf rust interaction
Authors: Koter Marek Daniel , Matuszkiewicz Mateusz , Rakoczy-Trojanowska Monika 
Published: January 2024

Abstract

Leaf rust (LR) caused by Puccinia recondita f. sp. secalis (Prs) is one of the most damaging diseases of rye. However, the genetic basis of the rye response to LR remains relatively unknown. Specifically, the involvement of small RNAs (sRNAs) in the rye–Prs interaction has not been characterized. In this study, the changes in various sRNAs in response to LR were revealed in three rye lines (D33, D39, and L318) that vary regarding their immune responses to LR and two Prs isolates that cause compatible (CP) or incompatible (ICP) interactions with the host. The sRNAs were analyzed using miRDeep2 and ShortStack algorithms, with true miRNAs detected on the basis of strict miRNA biosynthesis-related parameters. The differential expression of all sRNAs and miRNAs was analyzed and potential targets (i.e., mRNA) were detected. Additionally, the targets in the CP and ICP host–pathogen interactions were compared. Moreover, different isoforms of miRNAs originating from the same miRNA gene (isomiRs) were manually identified and the diversity in their potential targets were examined. The isomiRs can broaden the range of targets related to plant defense responses to pathogens. For example, UGUGUUCUCAGGUCGCCCCCG targets the transcripts of 11 endoglucanase genes and two superoxide dismutase genes, which encode proteins with plant defense-related functions. A shorter sequence (UCAGGUCGCCCCCGCUGGAG) was revealed to target 16 mRNAs encoding transcription factors, including those from the MADS box family. Finally, miRNA-like RNAs (milRNAs) from Prs and their predicted targets in rye were identified. To examine the effects of the experimental design, the differences in the number of milRNAs between CP and ICP interactions as well as the milRNA expression dynamics at two time-points were analyzed. Compared with the ICP interactions, the CP interactions resulted in more milRNAs, which affected many more genes, including some related to biotic stress responses.
Combination of atezolizumab and bevacizumab in patients with inoperable hepatocellular cancer in real clinical practice
Authors: Petkau V. V. , Shemetov D. Yu. , Semenova K. O. , Chubenko V. A. , Sultanbaev A. V. , Menshikov K. V. , Novikova O. Yu. , Orlova R. V. , Popova N. V. , Antipin A. S. , Mukhitova M. R. , Tarkhanov A. A. , Kiseleva K. E. 
Published: January 2024

Introduction. In the structure of Russian cancer incidence, malignant neoplasms (MN) of the liver occupy 1.61%. Patients with initially unresectable hepatocellular cancer (HCC) or progressive HCC after local treatment methods, in the absence of contraindications, are subject to systemic therapy.Aim. To evaluate the direct effectiveness and long-term results of treatment of patients with inoperable HCC with the combination of atezolizumab and bevacizumab in real clinical practice.Materials and methods. A multicenter (7 centers) retrospective observational study was conducted. It includes 56 previously untreated patients and 12 pretreated patients with a confirmed diagnosis of HCC who were treated with atezolizumab and bevacizumab. The male to female ratio was 2:1, with an average age of 60 years. ECOG 0-1 was in 86.8%, Child-Pugh liver function A – in 76.5%, B – in 23.5%, macroscopic portal vein invasion – in 27.9%, extrahepatic spread – in 35.3%, AFP more than 400 IU/ml – in 38.2%.Results. Use of the atezolizumab and bevacizumab regimen as a first line led to a partial response in 7 patients (12.5%), to stabilization in 39 (69.6%), to progression in 10 (17.9%). The disease control rate was 82.1%, median progression free survival (PFS) was 9.9 months (95% confidence interval (CI) 6.2-n/a). Median overall survival (OS) was not reached (95% CI 10.2-n/a). PFS was significantly influenced by the functional state of the liver according to the Child-Pugh scale. Median PFS in class A was 18.0 months, in class B – 5.6 months: HR 2.54; 95% CI 0.92–7.05; p = 0.03.Conclusion. The atezolizumab and bevacizumab regimen in real clinical practice demonstrates tolerability of therapy and treatment results that are not inferior to the data obtained in the registration study. No new adverse events were identified.
Clinical and economic evaluation of the use of enteral nutrition products
Authors: Loskutova E. E. , Kurashov M. M. , Radysh I. V. , Galkina G. A. , Savastenko A. L. , Klochkova S. V. 
Published: January 2024

Introduction. The development and study of new enteral nutrition (EN) formulas is a major challenge to modern practical medicine. The creation of enteral nutrition products, which in addition to their intrinsic nutritional properties have detoxifying properties, will contribute to enhanced clinical efficacy and more active recovery of patients.Aim. To conduct a comparative pharmaco-economic analysis of the effectiveness of using new domestic products for enteral nutrition produced by LEOVIT Nutrio (Russia), which have detoxifying activity, and products from other manufacturers.Materials and methods. The studies were carried out using standard cost-effectiveness analysis techniques. A comparative analysis of following products:Nutrien Standard (JSC Infaprim, Russia), Supportan drink (FreseniusKabi, Germany), Nutridrink compact protein (Nutricia, Holland) and Peptamen (Nestle, Switzerland), Detoxifying Protein Cocktail, Restoring Protein Cocktail and Detoxifying Drink (LEOVIT Nutrio LLC, Russia).Results and discussion. Calculations for all analyzed economic parameters showed the advantages of using new domestic products for enteral nutrition. It was found that the total direct costs for enteral nutrition in the group of cancer patients who additionally took a detoxification cocktail were the smallest and amounted to 3332.0 rub, the increase in blood protein concentration over 14 days of therapy was 11.1 g/l, and for albumin over the indicated period is 6.7 g/l. Compared to products from other manufacturers, the lowest cost in relation to the achieved effect (CER) was observed when using a restorative cocktail – 305.69 rub. for protein and 512.62 rub. by albumin. The total direct costs for enteral nutrition in the group of patients who took the Detoxification Drink in comparison with other products on the Russian market were also the lowest and amounted to 840.00 rubles.Conclusions. The authors concluded that there are significant economic advantages of using the new domestic enteral nutrition LEOVIT compared to other products and consider them as the product of choice when providing enteral protein nutrition to patients with various diseases accompanied by intoxication of the organism.
COVID-19 cases, vaccination, and SARS-CoV-2 in wastewater: insights from a Brazilian municipality
Authors: Abreu Mariana Aparecida de Freitas , Lopes Bruna Coelho , Assemany Paula Peixoto , Souza Aline dos Reis , Siniscalchi Luciene Alves Batista 
Published: January 2024

AbstractVaccines combatting COVID-19 demonstrate the ability to protect against disease and hospitalization, and reduce the likelihood of death caused by SARS-CoV-2. In addition, monitoring viral loads in sewage emerges as another crucial strategy in the epidemiological context, enabling early and collective detection of outbreaks. The study aimed to monitor the viral concentration of SARS-CoV-2 in untreated sewage in a Brazilian municipality. Also, it attempted to correlate these measurements with the number of clinical cases and deaths resulting from COVID-19 between July 2021 and July 2022. SARS-CoV-2 viral RNA was quantified by RT-qPCR. Pearson's correlation was performed to analyze the variables’ relationship using the number of cases, deaths, vaccinated individuals, and viral concentration of SARS-CoV-2. The results revealed a significant negative correlation (p &lt; 0.05) between the number of vaccinated individuals and the viral concentration of SARS-CoV-2, suggesting that after vaccination, the RNA viral load concentration was reduced in the sample population by the circulating concentration of wastewater. Consequently, wastewater monitoring, in addition to functioning as an early warning system for the circulation of SARS-CoV-2 and other pathogens, can offer a novel perspective that enhances decision-making, strengthens vaccination campaigns, and contributes to authorities establishing systematic networks for monitoring SARS-CoV-2.
Imaging and Histopathological Features Of Primary Thymic Neuroendocrine
Tumor
Authors: Suwal Sushant , Chen Ying-ying , Huang Sui-dan , Li Wei-feng , Chen Huai 
Published: January 2024

Objectives::
To investigate CT, MRI, and PET/CT features with histopathological findings of primary thymic neuroendocrine tumor.

Materials and Methods::
All 9 cases with pathologically proven primary thymic neuroendocrine tumors were reviewed retrospectively. Among them, 7 underwent enhanced
CT, 1 with MRI (enhanced) and another with PET/CT scan. Multiple characters were examined, including tumor location, contour, CT attenuation,
enhancement pattern, involvement of surrounding structure and lymphadenopathy.

Results::
Among 9 patients studied, 7 (77%) masses were located in the anterior superior mediastinum, 1 in the anterior superior-middle mediastinum, and 1
in the anterior and middle mediastinum. The maximum diameter (longitudinal) ranged from 4.2 to 23 cm (mean ± standard deviation, 9.5 cm ±
2.8). Four masses had irregular, 3 had lobulated, and 2 had smooth contours, while 8 masses had clear margins and 1 had an ill-defined margin. Six
masses showed heterogeneous attenuation with necrotic/cystic component (n=5), calcification (n=2) and hemorrhage(n=1), and 3 showed
homogeneous attenuation on the non-enhanced image. After contrast administration, 8 masses showed heterogeneous attenuation, and 1 showed
homogeneous attenuation with tumor vessels visible in 4 masses. Among all, 8 masses showed strong enhancement, and 1 showed moderate
enhancement in comparison to muscles in the anterior thoracic wall on enhanced images. Involvement of adjacent mediastinal structures was
observed in 5 cases. Immunohistochemical analysis showed that the tumor cells were positive for CgA, Syn, CK, CD56 and EMA.

Conclusion::
Primary NETs are large masses located anterior superior mediastinum, irregular in contour, showing heterogeneous attenuation with necrotic/cystic
component and strong heterogeneous enhancement with tumor vessels, compressing local mediastinal structures. In addition, immunohistochemical
examination is required in such a diagnosis.
Основные подходы к оценке эффективности корпоративной интеграции предприятий инвестиционно-строительного комплекса
Authors: Беляков Сергей Игоревич 
Published: January 2024

Важную роль в рамках управленческой деятельности традиционно выполняет функциональный блок оценки эффективности (в более широком смысле — результативности), определяющий подходы как к обоснованию управленческих решений, так и к их оценке. Современная российская и общемировая практика инвестиционно-строительной деятельности демонстрирует устойчивые тенденции к развитию разнообразных форм корпоративных объединений различных субъектов. Корпоративная интеграция в существенной мере может способствовать повышению устойчивости и эффективности функционирования субъектов, в том числе за счет синергетических эффектов интеграции. Понимание результативности деятельности организации в современном менеджменте значительно расширяется и не ограничивается только измерением достижения результата как части эффективности, а становится более системным и обобщающим понятием. Исходя из этого результативность деятельности может быть определена как комплексная многопараметрическая характеристика, отражающая способность организации достигать сбалансированных стратегических и тактических результатов, удовлетворяющих интересам и требованиям заинтересованных субъектов, с позиции повышения экономической эффективности, улучшения качества менеджмента, создания добавленной ценности, повышения конкурентоспособности организации в целях ее устойчивого развития. Проведенное автором исследование современных методологических подходов к определению результативности экономических систем в целом и субъектов инвестиционно-строительной деятельности в частности позволило выделить основные подходы к оценке эффективности корпоративной интеграции в инвестиционно-строительном комплексе в зависимости от превалирующих критериальных принципов и целевых векторов формирования эффективности как составляющей интегральной категории — результативности деятельности. Применение данной системы подходов в практике решения задач управления и оценки эффективности корпоративных структур может расширять трактовку эффективности и способствовать развитию управленческого инструментария.
Кластер как элемент стратегии реализации инвестиционно-строительных проектов в сфере индивидуального жилищного строительства
Authors: Ефимов Константин Владимирович 
Published: January 2024

В статье описан один из путей решения проблемы доступности жилья для регионов, где преобладает индивидуальное жилищное строительство. Согласно Национальному проекту «Жилье и городская среда», к 2030 г. ежегодный ввод жилья в стране должен составлять до 120 млн м2, из которых больше трети должно приходиться на индивидуальное жилищное строительство. Статистика показывает, что 68 % российских семей считают индивидуальный дом наиболее предпочтительным видом жилья. Переход на кластерную систему освоения территорий направлен на повышение эффективности и конкурентоспособности проектов в сфере индивидуального жилищного строительства. Идеология проектов кластерного освоения территорий предполагает создание общей социальной среды для всех жителей поселка, основной целью которой является: развитие коммерческой и социальной инфраструктуры внутри кластера. Основным примером такого подхода является инфраструктурный узел, расположенный внутри малоэтажной застройки. В рамках этого подхода несколько небольших поселков собраны вокруг одного ядра, внутри которого расположена коммерческая и социальная инфраструктура. Концепция кластерного освоения территорий позволит оптимизировать расходы на развитие инфраструктуры не только одного отдельно взятого поселения, но и будет способствовать развитию всей территории. Предполагается, что в будущем схема кластерного освоения территорий станет основным принципом девелопмента загородной недвижимости. Еще одним косвенным преимуществом кластерного освоения территорий является возможность для застройщика применять дополнительные методы по повышению рентабельности своих проектов. Наиболее распространенные из них — продажа готовых домов с отделкой, благоустройством, мебелью и дополнительными опциями. Так как в результате образования кластера формируется общее имущество, для управления которым нужна управляющая компания, для управления кластером индивидуального жилищного строительства (ИЖС) можно привлечь специализированную управляющую компанию, которая помимо основных услуг будет оказывать дополнительные услуги по содержанию приусадебных участков. Переход на кластерную схему освоения территорий позволит значительно повысить качество жизни людей, рассматривающих ИЖС как место для постоянного проживания.
Alfabetização: atuação docente e desafios no ensino remoto emergencial
Authors: Sibelly Soares Mendes Andrezza , Cavalcanti dos Santos Adriana 
Published: January 2024

Este artigo consiste em um recorte do projeto de pesquisa “Alfabetização em Alagoas no tempo/espaço do Ensino Remoto Emergencial: cenários e discursos” (SANTOS, 2020), que definiu por objetivo geral investigar a alfabetização no tempo/espaço do ensino remoto emergencial (ERE) em Alagoas na/pós pandemia com foco nos cenários político-pedagógicos e nos discursos de professores alfabetizadores. Foram analisados os dados quantitativos coletados nos municípios de Maceió e Arapiraca, ambos localizados no Estado de Alagoas. Os resultados indicam que a maioria dos docentes que participaram da pesquisa intervieram no ERE em turmas de alfabetização. Ademais, percebe-se que para os respondentes, as aulas remotas podem ser consideradas como uma solução de meio termo, indicando também que tiveram uma preparação razoável para o ERE. Os desafios englobam as dificuldades em conseguir fazer com que os alunos participassem das aulas. Referente às alternativas didáticas, os dados mostram que foram usadas diversas estratégias e plataformas digitais, constatando que a maioria dos docentes dispunha de acesso à internet e às ferramentas tecnológicas.
Dinapenia está associada a autopercepção de saúde negativa em pessoas idosas
Authors: Trindade Santos Kleyton , Fernandes Marcos Henrique , Alves Brito Thaís , Rocha de Sousa Halison Antony , Oliveira Carneiro José Ailton 
Published: January 2024

Objetivo: verificar a associação entre dinapenia e autopercepção de saúde em pessoas idosas residentes em comunidade. Métodos: estudo com delineamento transversal, realizado com 286 pessoas idosas, de ambos os sexos, residentes em comunidade. A autopercepção de saúde foi definida como variável dependente, e foi classificada em positiva, regular e negativa. A variável independente do estudo foi a dinapenia, verificada por meio do teste de força de preensão manual, de acordo com índice de massa corporal e sexo. Resultados: a prevalência de dinapenia foi de 28,7%. Foi possível verificar que 9,8% das pessoas idosas apresentavam uma autopercepção de saúde negativa. A dinapenia esteve associada de forma significativa a autopercepção de saúde negativa (OR: 3,05; IC: 1,31-7,08; p=0,010), aumentando em 3 vezes a chance da pessoa idosa se autodeclarar com uma saúde negativa se comparado as pessoas os idosas não dinapênicas (p=0,010). Conclusão: a presença de dinapenia estar associada a uma autopercepção de saúde negativa na pessoa idosa.