bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modification of Along‐Ridge Topography and Crustal Thickness by Mantle Plume and Oceanic Transform Fault at Ultra‐Slow Spreading Mohns Ridge
Authors: Zhang Yinuo , Zhang Fan , Zhang Xubo , Zhang Tao , Lin Jian , Zhou Zhiyuan , Zhang Jiangyang 
Published: December 2023

AbstractThe mantle plumes modify geophysical and geochemical features along and across mid‐ocean ridges. Despite abundant studies of plume‐ridge interaction, few geodynamic studies focus on the Arctic Ocean. The Jan Mayen Hotspot is located at the southern end of the Mohns Ridge and offset by the Jan Mayen Transform Fault, which creates an ideal area to study plume‐ridge‐transform fault interaction at the ultra‐slow spreading ridge. Through analyzing geophysical observations, we revealed that the M factor and crustal thickness decrease and the axial relief increases northeastward along the Mohns Ridge within a distance of ∼370 km to the Jan Mayen Hotspot. Combined with modeling results, the properties of the Jan Mayen plume were estimated, which has a diameter of 75 km, a temperature anomaly of 100°C, and a buoyancy flux of 0.22 Mg/s. Additionally, our model results indicate that the along‐ridge dispersion of plume is slightly enhanced by the transform fault.
Comparison of Cloud/Rain Band Structures of Typhoon Muifa (2022) Revealed in FY‐3E MWHS‐2 Observations With All‐Sky Simulations
Authors: Bi Mingming , Zou Xiaolei 
Published: December 2023

AbstractAll‐sky simulations of brightness temperature (TB) at Microwave Humidity Sounder‐2 (MWHS‐2) channel 15 from Fengyun‐3E (FY‐3E) are generated based on the European Center for Medium‐Range Weather Forecasts (ECMWF) reanalysis version 5 (ERA5) and the National Centers for Environmental Prediction (NCEP) Global Forecast System (GFS) analysis for four typhoon cases. For Typhoon Muifa (2022), the MWHS‐2 TB observations have a strongest symmetric component within about 210‐km radial distances. Although much weaker, the symmetric component in the GFS all‐sky simulations compares more favorably with that of observations than the ERA5 all‐sky simulations. Due to ice scattering effects at 183.31 ± 7 GHz, spatial distribution patterns of TB observations and all‐sky simulations are quite similar to those of ice water path. The more cloud ice, the lower the TB. Deep convective systems within Muifa are captured by TB observations but not by simulations. Using an objective azimuthal‐spectral‐analysis based center‐fixing algorithm, the center positions of Typhoon Muifa can be well determined from both MWHS‐2 observations and GFS all‐sky simulations, but not from the ERA5 all‐sky TB simulations. Compared with the best track, the average center position errors for Muifa are 16.2, 24.9 and 74.4 km based on FY‐3E MWHS‐2 TB observations, the GFS all‐sky TB simulations, and the ERA5 all‐sky simulations, respectively. The conclusions of TC cloud/rain band structures and center positions of all‐sky simulations using the NCEP‐GFS analysis being better matching with those of FY‐3E MWHS‐2 observations than the ERA5 all‐sky simulations for Typhoon Muifa also hold true for three other typhoons.
Human milk oligosaccharide composition following supplementation with folic acid vs (6S)-5-methyltetrahydrofolic acid during pregnancy and mediation by human milk folate forms
Authors: Cochrane Kelsey M. , Bone Jeffrey N. , Karakochuk Crystal D. , Bode Lars 
Published: December 2023

AbstractSupplementation with folic acid versus (6S)-5-methyltetrahydrofolic acid (5-MTHF) results in different folate forms in human milk, with folic acid increasing unmetabolized folic acid (UMFA) at the expense of reduced folate forms. It is unknown whether folate forms present in human milk have further effects on human milk composition, such as human milk oligosaccharide (HMO) concentrations. We randomized 60 pregnant women in Canada to 0.6 mg/day folic acid or (6S)-5-MTHF. Human milk folate forms (LC-MS/MS) and nineteen HMOs (HPLC) were quantified at 1 week postpartum. Linear regression and causal mediation analysis were used to evaluate the effect of folate supplementation on HMO concentrations, and possible mediation by concentrations of UMFA and reduced folate forms in human milk (controlling for secretor status and parity). HMO concentrations were not different between groups, with no evidence of mediation by reduced folate forms; however, increased UMFA was associated with reduced concentrations of total HMOs and 3’-sialyllactose.
Evolution and neutralization escape of the SARS-CoV-2 BA.2.86 subvariant
Authors: Khan Khadija , Lustig Gila , Römer Cornelius , Reedoy Kajal , Jule Zesuliwe , Karim Farina , Ganga Yashica , Bernstein Mallory , Baig Zainab , Jackson Laurelle , Mahlangu Boitshoko , Mnguni Anele , Nzimande Ayanda , Stock Nadine , Kekana Dikeledi , Ntozini Buhle , van Deventer Cindy , Marshall Terry , Manickchund Nithendra , Gosnell Bernadett I. , Lessells Richard J. , Karim Quarraisha Abdool , Abdool Karim Salim S. , Moosa Mahomed-Yunus S. , de Oliveira Tulio , von Gottberg Anne , Wolter Nicole , Neher Richard A. , Sigal Alex 
Published: December 2023

AbstractOmicron BA.2.86 subvariant differs from Omicron BA.2 as well as recently circulating variants by over 30 mutations in the spike protein alone. Here we report on the isolation of the live BA.2.86 subvariant from a diagnostic swab collected in South Africa which we tested for escape from neutralizing antibodies and viral replication properties in cell culture. We found that BA.2.86 does not have significantly more escape relative to Omicron XBB.1.5 from neutralizing immunity elicited by either Omicron XBB-family subvariant infection or from residual neutralizing immunity of recently collected sera from the South African population. BA.2.86 does have extensive escape relative to ancestral virus with the D614G substitution (B.1 lineage) when neutralized by sera from pre-Omicron vaccinated individuals and relative to Omicron BA.1 when neutralized by sera from Omicron BA.1 infected individuals. BA.2.86 and XBB.1.5 show similar viral infection dynamics in the VeroE6-TMPRSS2 and H1299-ACE2 cell lines. We also investigate the relationship of BA.2.86 to BA.2 sequences. The closest BA.2 sequences are BA.2 samples from Southern Africa circulating in early 2022. Similarly, many basal BA.2.86 sequences were sampled in Southern Africa. This suggests that BA.2.86 potentially evolved in this region, and that unobserved evolution led to escape from neutralizing antibodies similar in scale to recently circulating strains of SARS-CoV-2.
Myeloid cells interact with a subset of thyrocytes to promote their migration and follicle formation through NF-κB
Authors: Yang Rui-Meng , Song Shi-Yang , Wu Feng-Yao , Yang Rui-Feng , Shen Yan-Ting , Tu Ping-Hui , Wang Zheng , Zhang Jun-Xiu , Cheng Feng , Gao Guan-Qi , Liang Jun , Guo Miao-Miao , Yang Liu , Zhou Yi , Zhao Shuang-Xia , Zhan Ming , Song Huai-Dong 
Published: December 2023

AbstractThe pathogenesis of thyroid dysgenesis (TD) is not well understood. Here, using a combination of single-cell RNA and spatial transcriptome sequencing, we identify a subgroup of NF-κB-activated thyrocytes located at the center of thyroid tissues in postnatal mice, which maintained a partially mesenchymal phenotype. These cells actively protruded out of the thyroid primordium and generated new follicles in zebrafish embryos through continuous tracing. Suppressing NF-κB signaling affected thyrocyte migration and follicle formation, leading to a TD-like phenotype in both mice and zebrafish. Interestingly, during thyroid folliculogenesis, myeloid cells played a crucial role in promoting thyrocyte migration by maintaining close contact and secreting TNF-α. We found that cebpa mutant zebrafish, in which all myeloid cells were depleted, exhibited thyrocyte migration defects. Taken together, our results suggest that myeloid-derived TNF-α-induced NF-κB activation plays a critical role in promoting the migration of vertebrate thyrocytes for follicle generation.
Dictionary of immune responses to cytokines at single-cell resolution
Authors: Cui Ang , Huang Teddy , Li Shuqiang , Ma Aileen , Pérez Jorge L. , Sander Chris , Keskin Derin B. , Wu Catherine J. , Fraenkel Ernest , Hacohen Nir 
Published: December 2023

AbstractCytokines mediate cell–cell communication in the immune system and represent important therapeutic targets1–3. A myriad of studies have highlighted their central role in immune function4–13, yet we lack a global view of the cellular responses of each immune cell type to each cytokine. To address this gap, we created the Immune Dictionary, a compendium of single-cell transcriptomic profiles of more than 17 immune cell types in response to each of 86 cytokines (>1,400 cytokine–cell type combinations) in mouse lymph nodes in vivo. A cytokine-centric view of the dictionary revealed that most cytokines induce highly cell-type-specific responses. For example, the inflammatory cytokine interleukin-1β induces distinct gene programmes in almost every cell type. A cell-type-centric view of the dictionary identified more than 66 cytokine-driven cellular polarization states across immune cell types, including previously uncharacterized states such as an interleukin-18-induced polyfunctional natural killer cell state. Based on this dictionary, we developed companion software, Immune Response Enrichment Analysis, for assessing cytokine activities and immune cell polarization from gene expression data, and applied it to reveal cytokine networks in tumours following immune checkpoint blockade therapy. Our dictionary generates new hypotheses for cytokine functions, illuminates pleiotropic effects of cytokines, expands our knowledge of activation states of each immune cell type, and provides a framework to deduce the roles of specific cytokines and cell–cell communication networks in any immune response.
Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy
Authors: Ichbiah Sacha , Delbary Fabrice , McDougall Alex , Dumollard Rémi , Turlier Hervé 
Published: December 2023

AbstractTissue morphogenesis results from a tight interplay between gene expression, biochemical signaling and mechanics. Although sequencing methods allow the generation of cell-resolved spatiotemporal maps of gene expression, creating similar maps of cell mechanics in three-dimensional (3D) developing tissues has remained a real challenge. Exploiting the foam-like arrangement of cells, we propose a robust end-to-end computational method called ‘foambryo’ to infer spatiotemporal atlases of cellular forces from fluorescence microscopy images of cell membranes. Our method generates precise 3D meshes of cells’ geometry and successively predicts relative cell surface tensions and pressures. We validate it with 3D foam simulations, study its noise sensitivity and prove its biological relevance in mouse, ascidian and worm embryos. 3D force inference allows us to recover mechanical features identified previously, but also predicts new ones, unveiling potential new insights on the spatiotemporal regulation of cell mechanics in developing embryos. Our code is freely available and paves the way for unraveling the unknown mechanochemical feedbacks that control embryo and tissue morphogenesis.
Transport and inhibition mechanism of the human SGLT2–MAP17 glucose transporter
Authors: Hiraizumi Masahiro , Akashi Tomoya , Murasaki Kouta , Kishida Hiroyuki , Kumanomidou Taichi , Torimoto Nao , Nureki Osamu , Miyaguchi Ikuko 
Published: December 2023

AbstractSodium–glucose cotransporter 2 (SGLT2) is imporant in glucose reabsorption. SGLT2 inhibitors suppress renal glucose reabsorption, therefore reducing blood glucose levels in patients with type 2 diabetes. We and others have developed several SGLT2 inhibitors starting from phlorizin, a natural product. Using cryo-electron microscopy, we present the structures of human (h)SGLT2–MAP17 complexed with five natural or synthetic inhibitors. The four synthetic inhibitors (including canagliflozin) bind the transporter in the outward conformations, while phlorizin binds it in the inward conformation. The phlorizin–hSGLT2 interaction exhibits biphasic kinetics, suggesting that phlorizin alternately binds to the extracellular and intracellular sides. The Na+-bound outward-facing and unbound inward-open structures of hSGLT2–MAP17 suggest that the MAP17-associated bundle domain functions as a scaffold, with the hash domain rotating around the Na+-binding site. Thus, Na+ binding stabilizes the outward-facing conformation, and its release promotes state transition to inward-open conformation, exhibiting a role of Na+ in symport mechanism. These results provide structural evidence for the Na+-coupled alternating-access mechanism proposed for the transporter family.
Usefulness of barium sulfate and iohexol as contrast agents for VFSS in visualizing components of swallowing predictable of poor outcomes
Authors: Kang Min Soo , Chang Min Cheol , Kwak Soyoung 
Published: December 2023

AbstractBarium sulfate and iohexol are commonly used as contrast agents for videofluoroscopic swallowing study (VFSS). This study compared their usefulness as contrast agents in visualizing components of swallowing predictable of subsequent pneumonia and unintentional weight loss after VFSS. This was a randomized, controlled, crossover trial. The two contrast agents were alternately used in the same participants, and the order in which the contrast agent was tested first was randomly assigned. After VFSS, we followed the participants for 3 months and the association between VFSS findings of each contrast agent and the subsequent pneumonia and unintentional weight loss were analyzed. A total of 30 participants were included in the analysis. We recorded 11 cases of subsequent pneumonia and 13 of unintentional weight loss. Regarding the risk of subsequent pneumonia after VFSS, only the oral transit time and number of swallows tested with barium sulfate indicated significant differences between participants with and without subsequent pneumonia. For unintentional weight loss, oral transit time and pharyngeal wall coating after swallowing tested with barium sulfate, as well as oral transit time, nasal penetration, residue in the valleculae, PAS scores, and number of swallows when testing with iohexol demonstrated significant differences between those with and without unintentional weight loss.
Ecophysiological and behavioural response of juveniles of the Chilean cold-water coral Caryophyllia (Caryophyllia) huinayensis to increasing sediment loads
Authors: Fähse Melanie , Orejas Covadonga , Titschack Jürgen , Försterra Günter , Richter Claudio , Laudien Jürgen 
Published: December 2023

AbstractChilean Patagonia is a hotspot of biodiversity, harbouring cold-water corals (CWCs) that populate steep walls and overhangs of fjords and channels. Through anthropogenic activities such as deforestation, roadworks, aquafarming and increased landslide frequency, sediment input increases in the fjord region. While the absence of CWCs on moderately steep slopes has been suggested to reflect high vulnerability to sedimentation, experimental evidence has been lacking. Here, we investigated the sensitivity of CWCs to sediment stress, using juvenile Caryophyllia (Caryophyllia) huinayensis as a model. A 12-week aquarium experiment was conducted with three sediment loads: the average natural sediment concentration in Comau Fjord, 100- and 1000-fold higher sediment levels, expected from gravel road use and coastal erosion. Changes in coral mass and calyx dimensions, polyp expansion, tissue retraction and respiration were measured. For CWCs exposed to two and three order of magnitude higher sediment concentrations, 32% and 80% of the animals experienced a decrease in tissue cover, respectively, along with a decrease in respiration rate of 34% and 66%. Under the highest concentration corals showed reduced polyp expansion and a significantly reduced growth of ~ 95% compared to corals at natural concentration. The results show that C.huinayensis is affected by high sediment loads. As human activities that increase sedimentation steadily intensify, coastal planners need to consider detrimental effects on CWCs.
Cu2O, ZnO, and Ag/ Cu2O nanoparticles synthesized by biogenic and chemical route and their effect on Pseudomonas aeruginosa and Candida albicans
Authors: Rojas-Jaimes J. , Asmat-Campos David 
Published: December 2023

AbstractPseudomonas aeruginosa and Candida albicans are two important pathogens in public health due to the infections they cause in immunocompromised patients and with hospital stay, increasing morbimortality rates. Three groups of Cu2O, ZnO, and Ag/Cu2O nanoparticles were synthesized and characterized physicochemically and confronted to P. aeruginosa and C. albicans to determine their antibacterial effect. Statistical analyses were performed using Analysis of Variance (ANOVA) (p < 0.001). The structures of Cu2O, ZnO, and Ag/Cu2O nanoparticles were spherical, sized 6 nm, 10 nm, and 50 nm for Ag, Cu2, and Zn metals, respectively. Furthermore, a 100% antibacterial and antifungal effect against Pseudomonas aeruginosa and Candida albicans was observed for Cu2O, ZnO, and Ag/Cu2O nanoparticles respectively. It is concluded from these findings that the nanoparticles synthesized by biogenic and chemical route had a good size between 6 and 50 nm and that Cu2O, ZnO, and Ag/Cu2O nanoparticles presented an excellent antibacterial (100% growth inhibition) effect against P. aeruginosa and C. albicans (p < 0.001) compared to the control.
Muscle-driven simulations and experimental data of cycling
Authors: Clancy Caitlin E. , Gatti Anthony A. , Ong Carmichael F. , Maly Monica R. , Delp Scott L. 
Published: December 2023

AbstractMuscle-driven simulations have provided valuable insights in studies of walking and running, but a set of freely available simulations and corresponding experimental data for cycling do not exist. The aim of this work was to develop a set of muscle-driven simulations of cycling and to validate them by comparison with experimental data. We used direct collocation to generate simulations of 16 participants cycling over a range of powers (40–216 W) and cadences (75–99 RPM) using two optimization objectives: a baseline objective that minimized muscle effort and a second objective that additionally minimized tibiofemoral joint forces. We tested the accuracy of the simulations by comparing the timing of active muscle forces in our baseline simulation to timing in experimental electromyography data. Adding a term in the objective function to minimize tibiofemoral forces preserved cycling power and kinematics, improved similarity between active muscle force timing and experimental electromyography, and decreased tibiofemoral joint reaction forces, which better matched previously reported in vivo measurements. The musculoskeletal models, muscle-driven simulations, simulation software, and experimental data are freely shared at https://simtk.org/projects/cycling_sim for others to reproduce these results and build upon this research.
Incidence and burden of long COVID in Africa: a systematic review and meta-analysis
Authors: Frallonardo Luisa , Segala Francesco Vladimiro , Chhaganlal Kajal D. , Yelshazly Mohmaoud , Novara Roberta , Cotugno Sergio , Guido Giacomo , Papagni Roberta , Colpani Agnese , De Vito Andrea , Barbagallo Mario , Madeddu Giordano , Babudieri Sergio , Lochoro Peter , Ictho Jerry , Putoto Giovanni , Veronese Nicola , Saracino Annalisa , Di Gennaro Francesco 
Published: December 2023

AbstractLong COVID, also known as “post-acute sequelae of COVID-19,” affects at least 65 million individuals worldwide with a wide spectrum of symptoms that may last weeks, months, or permanently. Its epidemiology and burden in Africa are unclear. This meta-analysis examines long-term COVID-19 effects in the WHO African Region. A systematic search in several databases was carried out up to 12 February 2023 including observational studies from African countries reporting the cumulative incidence of long COVID signs and symptoms. Only studies conducted in African countries were included. Several sensitivity and meta-regression analyses were performed. Among 1547 papers initially screened, 25 were included, consisting of 29,213 participants. The incidence of any long COVID symptomatology was 48.6% (95% CI 37.4–59.8) as psychiatric conditions were the most frequent, particularly post-traumatic stress disorder reaching a cumulative incidence of 25% (95% CI 21.1–30.4). Higher age (p = 0.027) and hospitalization (p = 0.05) were associated with a higher frequency of long COVID. Long COVID poses a significant burden in Africa, particularly concerning psychiatric conditions. The study recommends identifying at-risk people and defining treatment strategies and recommendations for African long-COVID patients. High-quality studies addressing this condition in African setting are urgently needed.
Effects of air pollution on adverse birth outcomes and pregnancy complications in the U.S. state of Kansas (2000–2015)
Authors: Hao Hua , Yoo Sodahm R. , Strickland Matthew J. , Darrow Lyndsey A. , D’Souza Rohan R. , Warren Joshua L. , Moss Shannon , Wang Huaqing , Zhang Haisu , Chang Howard H. 
Published: December 2023

AbstractNeonatal mortality and morbidity are often caused by preterm birth and lower birth weight. Gestational diabetes mellitus (GDM) and gestational hypertension (GH) are the most prevalent maternal medical complications during pregnancy. However, evidence on effects of air pollution on adverse birth outcomes and pregnancy complications is mixed. Singleton live births conceived between January 1st, 2000, and December 31st, 2015, and reached at least 27 weeks of pregnancy in Kansas were included in the study. Trimester-specific and total pregnancy exposures to nitrogen dioxide (NO2), particulate matter with an aerodynamic diameter less than 2.5 μm (PM2.5), and ozone (O3) were estimated using spatiotemporal ensemble models and assigned to maternal residential census tracts. Logistic regression, discrete-time survival, and linear models were applied to assess the associations. After adjustment for demographics and socio-economic status (SES) factors, we found increases in the second and third trimesters and total pregnancy O3 exposures were significantly linked to preterm birth. Exposure to the second and third trimesters O3 was significantly associated with lower birth weight, and exposure to NO2 during the first trimester was linked to an increased risk of GDM. O3 exposures in the first trimester were connected to an elevated risk of GH. We didn’t observe consistent associations between adverse pregnancy and birth outcomes with PM2.5 exposure. Our findings indicate there is a positive link between increased O3 exposure during pregnancy and a higher risk of preterm birth, GH, and decreased birth weight. Our work supports limiting population exposure to air pollution, which may lower the likelihood of adverse birth and pregnancy outcomes.