bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between EPCR gene p.Ser219Gly polymorphism and venous thromboembolism risk: a case–control study, meta-analysis, and a reproducibility study
Authors: Pituk Dóra , Miklós Tünde , Schlammadinger Ágota , Rázsó Katalin , Bereczky Zsuzsanna 
Published: November 2023

BackgroundThe rs867186 single-nucleotide polymorphism in the PROCR gene (g.6936A &gt; G, c.4600A &gt; G) results in a serine-to-glycine substitution at codon 219 of endothelial protein C receptor (EPCR). We performed a case–control study followed by an updated meta-analysis of the association between this polymorphism and the risk of venous thromboembolism (VTE).Objective and methodsWe enrolled 263 VTE patients and 320 unrelated healthy controls for the case–control study. The total number of cases and controls for the meta-analysis were 5,768 and 30,017, respectively. A new online MetaGenyo Statistical Analysis System software was used to perform the current meta-analysis. Furthermore, a reproducibility study was conducted to validate our results.ResultsAmong well-defined thrombosis risk factors, Factor V Leiden was more frequent in the VTE group (p &lt; 0.001), while there was no difference in mutation frequency of prothrombin 20210G&gt;A polymorphism between the two groups. There was no difference in the mutation frequency of Factor V Leiden and prothrombin 20210G&gt;A between cases with and without provoking factors and cases with and without VTE recurrence. The rs867186 “G” carriership did not influence the risk of VTE [odds ratio (OR) 1.339; 95% confidence interval (CI): 0.904–1.984] in our study. No significant differences could be demonstrated among the rs867186 genotype frequencies between VTE cases with and without provoking factors (p = 0.430). PROCR rs867186 was associated with an OR of 1.72 (95% CI: 0.95–3.13, p = 0.075) in terms of VTE recurrence. In the meta-analysis, a significant association was found between EPCR Ser219Gly polymorphism and VTE under the dominant model (OR = 1.27, 95% CI: 1.11–1.46, p = 0.0006), the recessive model (OR = 1.60, 95% CI: 1.26–2.04, p = 0.0001), the GG vs. AA contrast model (OR = 1.64, 95% CI: 1.28–2.09, p = 0.0001), and the GA vs. AA contrast model (OR = 1.24, 95% CI: 1.08–1.43, p = 0.002).ConclusionThe rs867186 was not associated with the first VTE risk in our case–control study; however, a tendency to VTE recurrence was observed. Based on the results of our reproducibility study, MetaGenyo is acceptable for meta-analysis in case of genetic epidemiology studies. Although the risk conferred by the rs867186 is mild in all meta-analyses, including ours, identifying patients carrying the minor allele might have an impact on personalized VTE risk assessment, risk-score calculation, and patient management.
Targeting a cancer-specific LYPD3 glycoform for tumor therapy
Authors: Neumann Theresa , Hartung Evelyn , Gellert Johanna , Weiß Lisa , Weiske Manon , Kast Naomi , Gurka Stephanie , Marinoff Sophie , Jäkel Anika , Danielczyk Antje , Kehler Patrik 
Published: November 2023

Introduction: One of the most drastic changes in cancer is the altered glycosylation of proteins and lipids, giving rise to truncated O-glycans like the Thomsen Friedenreich (TF) or Thomsen nouvelle (Tn) antigen, which are almost absent on normal cells. Combined protein-carbohydrate epitopes comprising these specific glycans are ideal candidates for potent targeted therapies given their excellent tumor specificity and broad cancer expression.Methods and results: We have generated GT-002, a monoclonal antibody specifically targeting the epithelial glycoprotein LYPD3 only in the presence of a TF glycosylation. It does not cross-react with non-glycosylated LYPD3 or TF on other glycoproteins in ELISA and flow cytometry. GT-002 binds to various tumor cell lines and stains tumor tissues of different cancer indications including squamous cell carcinoma of the head and neck. The remarkable tumor specificity was confirmed in an immunohistochemistry study on a normal human tissue panel including several LYPD3-positive organs, where GT-002 elicited almost completely abolished normal tissue binding. Consequently, we observed markedly reduced binding of GT-002 to normal human tissues compared to Lupartumab, a conventional anti-LYPD3 antibody previously in clinical development as antibody-drug conjugate (BAY1129980). Neuraminidase treatment of healthy tissues, resulting in cleavage of sialic acid residues, re-established binding of GT-002 comparable to Lupartumab, showing that the GT-002 epitope is masked by sialic acid in normal cells.Discussion: We believe that GT-002 is a promising candidate for development of antibody-drug- and radio-conjugates as well as bispecific molecules and chimeric antigen receptor therapeutics and highlights the powerful potential of antibodies against combined protein-carbohydrate epitopes to reduce on-target/off-tumor cytotoxicity.
Recent and episodic activity of decoupled mud/fluid discharge at Sartori mud volcano in the Calabrian Arc, Mediterranean Sea
Authors: Doll Mechthild , Römer Miriam , Pape Thomas , Kölling Martin , Kaul Norbert , dos Santos Ferreira Christian , Bohrmann Gerhard 
Published: November 2023

Mud volcanoes (MVs) are surface structures typically created by episodic discharge of fluids and solids, often associated to onshore and offshore accretionary prisms on convergent plate boundaries. Detailed investigations of ongoing activity and its associated morphological changes, as well as a better understanding of the temporal evolution of these highly dynamic systems, may improve the estimations of material fluxes from MVs drastically. Until today, approximately 70 individual MVs were discovered in the northern Ionian Sea in the Calabrian Arc (Central Mediterranean Sea), but only a few have been analyzed and described in detail. In this study, new evidence for recent recurring eruptive activity of the ∼45 m-high and 1 km-wide Sartori MV situated in the clastic wedge of the Calabrian Arc is presented. High-resolution seafloor mapping as well as sediment temperature, geochemical, and sedimentological data received from two research cruises in 2016 and 2020 are used. Bathymetric and seafloor backscatter data (1 m scale) indicate the presence of two active eruption centers at the flat-topped Sartori MV. Elevated sediment temperature gradients at both eruption centers show that currently heat is transferred to the surface sediments. Pore water analyses indicate that fluids rising below the eruption centers are CH4-rich, Cl−-poor, and SO42−-free. Stable C and H isotopic compositions of methane suggest that it originates from a mix of primary microbial, secondary microbial, and/or thermogenic sources. A relatively shallow position of the sulfate–methane interface at both eruption centers also indicates the presence of upward fluid migration in recent times. Pore water modeling suggests that seawater has penetrated the surface sediments to a greater extent within the last few years. In contrast, centimeter-thick layers of hemipelagic sediments overlying mud breccia in sediment cores taken from both eruption centers show that no solid material has been ejected in recent times. Sediment core analyses combined with high-resolution seafloor mapping show an absence of rim-passing mudflows over the past ∼10 ka. It is concluded that Sartori MV is an episodically active MV from which fluids with a comparatively low flux were released into the bottom water in recent times.
Tectonic and hydrothermal activity at the Yellow Lake fissure in response to the 2004 Dallol dyke intrusion event in Afar
Authors: Mège Daniel , Hauber Ernst , Dyment Jérôme , Allemand Pascal , Moors Hugo , De Craen Mieke , Choe Hanjin 
Published: November 2023

The Danakil depression in Ethiopia, at the southern end of the Red Sea, has been the place of volcanic crises in 2004–10, with emplacement of at least 15 dykes. One of them, non-emergent, occurred in dry lake Asale next to Black Mountain and south of Mount Dallol during fall 2004. We report on the opening of a 4.5 km-long fissure in the ground at the same time the Black Mountain dyke was intruding the crust 2 km westward and parallel to it. The fissure, located north and south of Yellow Lake (Gaet’ale) and trending NNW-SSE, is still hydrothermally active. First, we describe the remarkable diversity of morphologic expressions of the fissure, made possible by development in an evaporite sequence. Satellite image monitoring reveals that its formation is coeval with the latest intrusion stage of the Black Mountain dyke. Hydrothermal activity in the fissure area is, however older than ∼60 years. It is suggested that hydrothermal activity is primarily a side effect of the igneous processes, probably sill intrusion, that resulted in the uplift of Mount Dallol area, in a ∼400 m thick, fluid-saturated evaporite pile. We suggest that, in 2004, emplacement of the Black Mountain dyke caused dilation within the evaporite pile overlying it, where extension was also facilitated by pressured pore fluids. This study documents the delicate intermingling of magmatic, tectonic, hydrothermal, and geomorphologic processes in evaporitic environments at the transition between continental rifting and oceanic spreading.
Correlation of executive functions, academic achievement, eating behavior and eating habits in university students of Mexico City
Authors: Chávez-Hernández María Elena 
Published: November 2023

IntroductionYoung adults between 18 and 25 years of age are in an important transitional time and university students are considered vulnerable given that they generally maintain an unhealthy lifestyle characterized by poor diet quality and meal skipping. The aim of the present study was to evaluate the relationship between executive functions, academic achievement, eating behavior, eating habits, and BMI in university students.MethodsUndergraduate university students (18–25 years) who lived in Mexico City were included in the sample; executive function, eating habits, eating behavior and academic achievement were measured.Results1903 university students (1,038 women, 865 men), with a mean age of 20.65 years (SD ±1.66), participated in the study. Spearman’s r correlation analysis revealed that there is a significant correlation between all variables of interest, with the most significant correlations found with academic achievement, emotional eating, and eating habits.DiscussionOur study confirms previous findings in the association between EF, eating habits, eating behavior, and academic achievement in university students, and also provides a first approach to the association between UP food intake, overall meal skipping (breakfast, lunch, and/or dinner) and EF.
Advantages of total parathyroidectomy in patients with secondary hyperparathyroidism induced by end stage renal disease
Authors: Iorga Cristian , Iorga Cristina Raluca , Andreiana Iuliana , Bengulescu Iustinian , Constantin Traian , Strambu Victor 
Published: November 2023

IntroductionSecondary hyperparathyroidism, as a result of chronic kidney disease could be treated medically or surgically. When pharmacotherapy fails, patients undergo surgery - parathyroidectomy, the curative treatment of secondary hyperparathyroidism (SHPT). There are currently 3 accepted surgical techniques, each with supporters or opponents – total parathyroidectomy, subtotal parathyroidectomy and parathyroidectomy with immediate autotransplantation.MethodsIn this paper we described our experience on a series of 160 consecutive patients diagnosed with secondary hyperparathyroidism who underwent surgery, in 27 cases it was totalization of the intervention (patients with previously performed subtotal parathyroidectomy or with supernumerary glands and SHPT recurrence). We routinely perform total parathyroidectomy, the method that we believe offers the best results. ResultsThe group of patients was studied according to demographic criteria, paraclinical balance, clinical symptomatology, pre- and postoperative iPTH (intact parathormone) values, SHPT recurrence, number of reinterventions. In 31 cases we found gland ectopy and in 15 cases we discovered supernumerary parathyroids. A percentage of 96.24% of patients with total parathyroidectomy did not show recurrence.DiscussionsAfter analyzing the obtained results, our conclusion was that total parathyroidectomy is the intervention of choice for patients suffering from secondary hyperparathyroidism when pharmacotherapy fails in order to prevent recurrence of the disease and to correct the metabolic parameters.
Multi-view information fusion using multi-view variational autoencoder to predict proximal femoral fracture load
Authors: Zhao Chen , Keyak Joyce H. , Cao Xuewei , Sha Qiuying , Wu Li , Luo Zhe , Zhao Lan-Juan , Tian Qing , Serou Michael , Qiu Chuan , Su Kuan-Jui , Shen Hui , Deng Hong-Wen , Zhou Weihua 
Published: November 2023

BackgroundHip fracture occurs when an applied force exceeds the force that the proximal femur can support (the fracture load or “strength”) and can have devastating consequences with poor functional outcomes. Proximal femoral strengths for specific loading conditions can be computed by subject-specific finite element analysis (FEA) using quantitative computerized tomography (QCT) images. However, the radiation and availability of QCT limit its clinical usability. Alternative low-dose and widely available measurements, such as dual energy X-ray absorptiometry (DXA) and genetic factors, would be preferable for bone strength assessment. The aim of this paper is to design a deep learning-based model to predict proximal femoral strength using multi-view information fusion.ResultsWe developed new models using multi-view variational autoencoder (MVAE) for feature representation learning and a product of expert (PoE) model for multi-view information fusion. We applied the proposed models to an in-house Louisiana Osteoporosis Study (LOS) cohort with 931 male subjects, including 345 African Americans and 586 Caucasians. We performed genome-wide association studies (GWAS) to select 256 genetic variants with the lowest p-values for each proximal femoral strength and integrated whole genome sequence (WGS) features and DXA-derived imaging features to predict proximal femoral strength. The best prediction model for fall fracture load was acquired by integrating WGS features and DXA-derived imaging features. The designed models achieved the mean absolute percentage error of 18.04%, 6.84% and 7.95% for predicting proximal femoral fracture loads using linear models of fall loading, nonlinear models of fall loading, and nonlinear models of stance loading, respectively.ConclusionThe proposed models are capable of predicting proximal femoral strength using WGS features and DXA-derived imaging features. Though this tool is not a substitute for predicting FEA using QCT images, it would make improved assessment of hip fracture risk more widely available while avoiding the increased radiation exposure from QCT.
Development and external validation of dual online tools for prognostic assessment in elderly patients with high-grade glioma: a comprehensive study using SEER and Chinese cohorts
Authors: Zhang Hongyu , Jiang Xinzhan , Ren Fubin , Gu Qiang , Yao Jiahao , Wang Xinyu , Zou Shuhuai , Gan Yifan , Gu Jianheng , Xu Yongji , Wang Zhao , Liu Shuang , Wang Xuefeng , Wei Baojian 
Published: November 2023

BackgroundElderly individuals diagnosed with high-grade gliomas frequently experience unfavorable outcomes. We aimed to design two web-based instruments for prognosis to predict overall survival (OS) and cancer-specific survival (CSS), assisting clinical decision-making.MethodsWe scrutinized data from the SEER database on 5,245 elderly patients diagnosed with high-grade glioma between 2000-2020, segmenting them into training (3,672) and validation (1,573) subsets. An additional external validation cohort was obtained from our institution. Prognostic determinants were pinpointed using Cox regression analyses, which facilitated the construction of the nomogram. The nomogram’s predictive precision for OS and CSS was gauged using calibration and ROC curves, the C-index, and decision curve analysis (DCA). Based on risk scores, patients were stratified into high or low-risk categories, and survival disparities were explored.ResultsUsing multivariate Cox regression, we identified several prognostic factors for overall survival (OS) and cancer-specific survival (CSS) in elderly patients with high-grade gliomas, including age, tumor location, size, surgical technique, and therapies. Two digital nomograms were formulated anchored on these determinants. For OS, the C-index values in the training, internal, and external validation cohorts were 0.734, 0.729, and 0.701, respectively. We also derived AUC values for 3-, 6-, and 12-month periods. For CSS, the C-index values for the training and validation groups were 0.733 and 0.727, with analogous AUC metrics. The efficacy and clinical relevance of the nomograms were corroborated via ROC curves, calibration plots, and DCA for both cohorts.ConclusionOur investigation pinpointed pivotal risk factors in elderly glioma patients, leading to the development of an instrumental prognostic nomogram for OS and CSS. This instrument offers invaluable insights to optimize treatment strategies.
Experimental study on flue gas foam-assisted steam flooding: investigating characteristics of enhanced oil recovery and gas storage
Authors: Cao Qiuying , Wei Tao , Wu Guanghuan , Yu Jianmei , Tian Kehan , Cao Aiqing 
Published: November 2023

Steam flooding is one of the most widely used heavy oil thermal recovery technologies. Steam transfers heat to heavy oil to reduce viscosity and improve fluidity. The current problem is that steam loses a lot of heat in the formation, and there are serious carbon emissions in the whole production process. In this paper, flue gas and steam were combined to drive heavy oil in the form of composite thermal fluid, and foam was added on this basis. With the help of one-dimensional sandpack model, both single-model and parallel dual-model with permeability ratio experiments were conducted to investigate key characteristics such as steam heat transfer, heavy oil production and flue gas retention during the displacement process. The experimental results indicated that flue gas effectively inhibited steam condensation and reduced heat loss during the flow process. Compared to steam flooding, the sandpack model exhibited temperature rises of 4.4°C and 9.1°C at the middle and end, respectively. While flue gas foam fell slightly short of flue gas in terms of enhanced heat transfer, it outperforms in recovery factor, achieving a 10.4% improvement over flue gas-assisted steam flooding. The foam blocked gas channeling by accumulating and capturing along the flow path, resulting in a gas retention volume of 389 mL within the model. Furthermore, the flue gas foam facilitated steam flow to previously unswept low-permeability areas, thus enhancing oil recovery. In the parallel double-model experiment, the low-permeability model exhibited significantly improved oil displacement efficiency compared to flue gas-assisted steam flooding, and the remaining oil content in the end of the high permeability model was increased by 1.9%, while the remaining oil content in the front and end of the low-permeability model was reduced by 3.5% and 3.8% respectively.
Diversity and soil chemical properties jointly explained the basal area in karst forest
Authors: Lu Lihong , Zeng Fuping , Zeng Zhaoxia , Du Hu , Zhang Chen , Zhang Hao 
Published: November 2023

IntroductionPlant diversity and soil chemical properties are important factors affecting the plant growth. We sought to compare the explanatory rates of diversity and soil chemical properties in explaining the variation of basal area in karst forests, and also sought to compare the relative importance of the niche complementarity and mass ratio hypotheses.MethodsOn the basis of linear regression and structural equation modelling, we examined the correlation between the basal area of plant communities and species diversity, functional diversity, phylogenetic diversity, the community-weighted mean (CWM) of traits, and soil chemical properties, using data obtained from 35 monitoring plots in southwest China.ResultsSpecies, functional, and phylogenetic diversities were all significantly correlated with the basal area of the plant community, among the indices of which, Faith’s phylogenetic diversity was found to have the greatest explanatory power for basal area. These plant diversity indices can better explain the variation in basal area than the CWM of traits, suggesting the niche complementarity hypothesis is more applicable than the mass ratio hypothesis. Moreover, soil chemical properties also have an equal important impact. Different chemical properties were found to show significant positive correlations with basal area, and their total effects on basal area were shown to be greater than the CWM of traits.DiscussionAttention should be paid to diversity and soil chemical properties. This study provides theoretical guidance for understanding biodiversity maintenance mechanisms and protecting karst forests.
Mixed Eucalyptus plantations in subtropical China enhance phosphorus accumulation and transformation in soil aggregates
Authors: Cui Yuhong , Yan Yu , Wang Shengqiang , Zhang Han , He Yaqin , Jiang Chenyang , Fan Rongyuan , Ye Shaoming 
Published: November 2023

IntroductionThe production of Eucalyptus, a principal economic tree genus in China, is faced with challenges related to soil phosphorus (P) limitations. In this study, we explore variations in phosphorus content, storage, and transformation in Eucalyptus forests. We hypothesize that mixed forests augment soil aggregate stability and P content and that microaggregates are pivotal in determining P differences between mixed and pure forests. Additionally, we posit that mixed forests foster P transformation, enhancing its efficacy in the soil. Current research on the distribution and transformation of soil total P (TP) and P fractions at the soil aggregate level is limited.MethodsIn this study, we selected soil from a Eucalyptus-Mytilaria laosensis Lecomte mixed forest, Eucalyptus-Erythrophleum fordii Oliv mixed forest, and pure Eucalyptus forest in Chongzuo County, Guangxi, China, as the research objects. Using a dry-sieving method, we divided the soil collected in situ from the 0–40 cm layer into aggregates of &gt;2, 1–2, 0.25–1, and &lt;0.25 mm particle sizes, measured the TP and P fractions (resin-extractable inorganic P, bicarbonate-extractable inorganic P, bicarbonate-extractable organic P, sodium hydroxide-extractable inorganic P, sodium hydroxide-extractable organic P, dilute hydrochloric acid-extractable P, concentrated hydrochloric acid extractable inorganic P, concentrated hydrochloric acid-extractable organic P and residue-P) in different aggregates, and used redundancy analysis and PLS SEM to reveal key factors affecting soil P accumulation and transformation.ResultsThe results showed that compared to pure Eucalyptus forests, mixed Eucalyptus forests significantly enhanced the stability of soil aggregates and the content and storage of phosphorus, especially the Eucalyptus-Mytilaria laosensis mixed forest. The content of total soil phosphorus and its fractions decreased with increasing aggregate particle size, while the opposite trend was observed for stored P, with aggregates &lt;0.25 mm being the main fraction influencing soil phosphorus accumulation. The transformation process of P fractions was primarily constrained by dissolution rates, mineralization rates, biological activity, including the action of microbes, fungi, and plant–root interactions, and other factors.DiscussionMixed forests increased the transformation of phosphorus in soil aggregates, effectivel enhancing the availability of soil phosphorus. In summary, this study provides important evidence for the systematic management of subtropical artificia Eucalyptus forests and the sustainable utilization of soil resources.