bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leveraging a disulfidptosis‑related lncRNAs signature for predicting the prognosis and immunotherapy of glioma
Authors: Chen Di , Li Qiaoqiao , Xu Yuan , Wei Yanfei , Li Jianguo , Zhu Xuqiang , Li Hongjiang , Lu Yan , Liu Xianzhi , Yan Dongming 
Published: December 2023

Abstract
Background
Gliomas, a prevalent form of primary brain tumors, are linked with a high mortality rate and unfavorable prognoses. Disulfidptosis, an innovative form of programmed cell death, has received scant attention concerning disulfidptosis-related lncRNAs (DRLs). The objective of this investigation was to ascertain a prognostic signature utilizing DRLs to forecast the prognosis and treatment targets of glioma patients.

Methods
RNA-seq data were procured from The Cancer Genome Atlas database. Disulfidptosis-related genes were compiled from prior research. An analysis of multivariate Cox regression and the least absolute selection operator was used to construct a risk model using six DRLs. The risk signature’s performance was evaluated via Kaplan-Meier survival curves and receiver operating characteristic curves. Additionally, functional analysis was carried out using GO, KEGG, and single-sample GSEA to investigate the biological functions and immune infiltration. The research also evaluated tumor mutational burden, therapeutic drug sensitivity, and consensus cluster analysis. Reverse transcription quantitative PCR was conducted to validate the expression level of DRLs.

Results
A prognostic signature comprising six DRLs was developed to predict the prognosis of glioma patients. High-risk patients had significantly shorter overall survival than low-risk patients. The robustness of the risk model was validated by receiver operating characteristic curves and subgroup survival analysis. Risk model was used independently as a prognostic indicator for the glioma patients. Notably, the low-risk patients displayed a substantial decrease in the immune checkpoints, the proportion of immune cells, ESTIMATE and immune score. IC50 values from the different risk groups allowed us to discern three drugs for the treatment of glioma patients. Lastly, the potential clinical significance of six DRLs was determined.

Conclusions
A novel six DRLs signature was developed to predict prognosis and may provide valuable insights for patients with glioma seeking novel immunotherapy and targeted therapy.
Single-cell RNA sequencing in cancer research: discovering novel biomarkers and therapeutic targets for immune checkpoint blockade
Authors: Sun Boyu , Xun Ziyu , Zhang Nan , Liu Kai , Chen Xiangqi , Zhao Haitao 
Published: December 2023

AbstractImmune checkpoint blockade (ICB) has become a promising strategy in treating advanced cancers, providing significant survival benefits for patients with various cancer types. However, among the vast population of cancer patients, only a small fraction are able to respond to and derive benefits from ICB therapy. Numerous factors contribute to the diminished efficacy of ICB, with the complex tumor microenvironment (TME) playing an important role. Therefore, comprehensively understanding the intricate composition of the TME is critical for elucidating the mechanisms that underlie distinct responses to ICB in patients. Single-cell RNA sequencing (scRNA-seq) is a novel technique that reveals gene expression profiles of individual cells, facilitating the investigation of TME heterogeneity at a high resolution and the identification of key cell subsets participating in the response to ICB. This review emphasizes the importance of scRNA-seq in studying ICB and summarizes recent findings in the discovery of biomarkers that predict ICB response and novel potential therapeutic targets for immunotherapy. These findings suggest future directions for the clinical implementation of cancer immunotherapy, facilitating further advancements in precision medicine.
Association of air pollution exposure and increased coronary artery disease risk: the modifying effect of genetic susceptibility
Authors: Fu Zuqiang , Ma Yuanyuan , Yang Changjie , Liu Qian , Liang Jingjia , Weng Zhenkun , Li Wenxiang , Zhou Shijie , Chen Xiu , Xu Jin , Xu Cheng , Huang Tao , Zhou Yong , Gu Aihua 
Published: December 2023

Abstract
Background
Both genetic factors and air pollution are risk factors for coronary artery disease (CAD), but their combined effects on CAD are uncertain. The study aimed to comprehensively investigate their separate, combined and interaction effects on the onset of CAD.

Methods
We utilized data from the UK Biobank with a recruitment of 487,507 participants who were free of CAD at baseline from 2006 to 2010. We explored the separate, combined effect or interaction association among genetic factors, air pollution and CAD with the polygenic risk score (PRS) and Cox proportional hazard models.

Results
The hazard ratios (HRs) [95% confidence interval (CI)] of CAD for 10-µg/m3 increases in PM2.5, NO2 and NOx concentrations were 1.25 (1.09, 1.44), 1.03 (1.01, 1.05) and 1.01 (1.00, 1.02), respectively. Participants with high PRS and air pollution exposure had a higher risk of CAD than those with the low genetic risk and low air pollution exposure, and the HRs (95% CI) of CAD in the PM2.5, PM10, NO2 and NOx high joint exposure groups were 1.56 (1.48, 1.64), 1.55(1.48, 1.63), 1.57 (1.49, 1.65), and 1.57 (1.49, 1.65), respectively. Air pollution and genetic factors exerted significant additive effects on the development of CAD (relative excess risk due to the interaction [RERI]: 0.12 (0.05, 0.19) for PM2.5, 0.17 (0.10, 0.24) for PM10, 0.14 (0.07, 0.21) for NO2, and 0.17 (0.10, 0.24) for NOx; attributable proportion due to the interaction [AP]: 0.09 (0.04, 0.14) for PM2.5, 0.12 (0.07, 0.18) for PM10, 0.11 (0.06, 0.16) for NO2, and 0.13 (0.08, 0.18) for NOx).

Conclusion
Exposure to air pollution was significantly related to an increased CAD risk, which could be further strengthened by CAD gene susceptibility. Additionally, there were positive additive interactions between genetic factors and air pollution on the onset of CAD. This can provide a more comprehensive, precise and individualized scientific basis for the risk assessment, prevention and control of CAD.
Geographical and practical challenges in the implementation of digital health passports for cross-border COVID-19 pandemic management: a narrative review and framework for solutions
Authors: Towett Gideon , Snead R. Sterling , Grigoryan Knarik , Marczika Julia 
Published: December 2023

AbstractThe rapid global spread of infectious diseases, epitomized by the recent COVID-19 pandemic, has highlighted the critical need for effective cross-border pandemic management strategies. Digital health passports (DHPs), which securely store and facilitate the sharing of critical health information, including vaccination records and test results, have emerged as a promising solution to enable safe travel and access to essential services and economic activities during pandemics. However, the implementation of DHPs faces several significant challenges, both related to geographical disparities and practical considerations, necessitating a comprehensive approach for successful global adoption. In this narrative review article, we identify and elaborate on the critical geographical and practical barriers that hinder global adoption and the effective utilization of DHPs. Geographical barriers are complex, encompassing disparities in vaccine access, regulatory inconsistencies, differences across countries in data security and users' privacy policies, challenges related to interoperability and standardization, and inadequacies in technological infrastructure and limited access to digital technologies. Practical challenges include the possibility of vaccine contraindications and breakthrough infections, uncertainties surrounding natural immunity, and limitations of standard tests in assessing infection risk. To address geographical disparities and enhance the functionality and interoperability of DHPs, we propose a framework that emphasizes international collaboration to achieve equitable access to vaccines and testing resources. Furthermore, we recommend international cooperation to establish unified vaccine regulatory frameworks, adopting globally accepted standards for data privacy and protection, implementing interoperability protocols, and taking steps to bridge the digital divide. Addressing practical challenges requires a meticulous approach to assessing individual risk and augmenting DHP implementation with rigorous health screenings and personal infection prevention measures. Collectively, these initiatives contribute to the development of robust and inclusive cross-border pandemic management strategies, ultimately promoting a safer and more interconnected global community in the face of current and future pandemics.
Pre-treatment optimisation with pulmonary rehabilitation of elderly lung cancer patients with frailty for surgery
Authors: Goldsmith Ira , Chesterfield-Thomas Gemma , Toghill Hannah 
Published: December 2023

Abstract
Objective
Frailty develops as a result of age-related decline in many physiological systems and is associated with increased vulnerability to adverse outcomes following thoracic surgery. We prospectively tested our hypothesis that pre-operative pulmonary rehabilitation (Prehab) improves frailty, as suggested by a frailty index > 3 (FI > 3) and fitness, and thereby reduces the risk of post-surgical complications and death in vulnerable elderly lung cancer patients.

Methods
221 surgical patients, 80 with FI > 3 vs. 141 patients with FI < 3, following Prehab proceeded to surgery. Their Frailty index (FI), dyspnoea scores, performance status (PS), level of activity (LOA) and six-minute walk test (6MWT) prior to and following Prehab were determined. The post-operative length of hospital stay (LOHS), complications, mortality and mid-term survival at 1100 days were compared. Similarly, outcomes for elderly patient ≥ 70 years with FI > 3 (≥ 70,FI > 3) were compared with younger patients < 70 years with FI ≤ 3 (< 70,FI ≤ 3).

Results
Patients with FI > 3 were significantly older, had lower 6MWT and higher thoracoscores hence, 82.5% of patients with FI > 3 vs. 33.3% (p = 0.02) with FI ≤ 3 were considered high risk for surgery and postoperative adverse events. With Prehab there was significant improvement in the FI, dyspnoea scores, PS, LOA and 6MWT. Following surgery, there were no differences in major complication rates (8.8% vs. 9.2% p = ns); LOHS median (IQR) [7 (6.8) vs. 8 (5.5) days]; mortality at 30-days (3.7% vs. 0.7%, p = ns); 90-days (6.3% vs. 2.8%, p = ns) and 1-year survival (81.1% vs. 83.7% p = ns). Survival at 1100 days was (63.2% vs. 71.1%, p = 0.19). Likewise, 87.7% elderly ≥ 70,FI > 3 patients were considered high-risk for surgery and postoperative adverse events vs. 35.1% younger patients < 70,FI ≤ 3 (p = 0.0001). Following Prehab and surgery, there were no significant differences in complications, LOHS, mortality at 365 days between the two groups. Survival at 1100 days for ≥ 70,FI > 3 was 55.2% vs. 79.96% for < 70,FI ≤ 3; (p = 0,01).

Conclusion
Our study suggests that Prehab optimises vulnerable high-risk elderly lung cancer patients with frailty allowing them to undergo surgery with outcomes of post-surgical complications, LOHS and mortality at 365 days no different to patients with no frailty. However, mid-term survival was lower for elderly patients with frailty.
Quality of reporting of integrative Chinese and Western medicine intervention in randomized controlled trials of ulcerative colitis: a review
Authors: Zhang Jialing , Deng Jiashuai , Wang Nana , Wang Ping , Li Ji , Wang Yunhai , Cui Wanting , Liang Feng , Chen Peijin , Wang Juan , Han Fei , Chan Chun Pong , Lyu Aiping , Bian Zhaoxiang , Zhang Xuan 
Published: December 2023

Abstract
Background
Integrative Chinese and Western medicine (ICWM) is commonly used for the treatment of ulcerative colitis (UC) in clinical practice. However, it is unclear whether the details of ICWM interventions, such as selection rationale, implementation design, and potential interactions, were adequately reported. Therefore, this study aimed to assess the quality of reporting in the ICWM interventional randomized controlled trials (RCTs) of UC and to identify the common problems if any.

Methods
Through a search of 10 international electronic databases, we identified RCTs of UC with ICWM interventions published in English or Chinese from the inception date of each database up to 16 June 2023. Literature screening was strictly conducted based on the inclusion and exclusion criteria of the Population, Concept, and Context (PCC) framework. The general characteristics of the included studies were described. The quality of reporting was assessed according to three checklists, including the CONSORT (Consolidated Standards of Reporting Trials) with 36 items (except for one item 1b about abstract), the CONSORT for Abstracts (17 items), and a self-designed ICWM-related checklist (27 items covering design rationale, intervention details, outcome assessments, and analysis). The reporting scores of RCTs published before and after 2010 were compared.

Results
A total of 1458 eligible RCTs were included. For the reporting compliance, the median score (interquartile ranges) of the CONSORT (72 score in total), the CONSORT for Abstract (34 score), and ICWM-related (54 score) items was 21 (18–25), 13 (12–15), and 18 (15–21), respectively. Although the time period comparisons showed that reporting quality of included publications improved significantly after the CONSORT 2010 issued (P < 0.01), more than 50% of items were evaluated as poor quality (reporting rate < 65%) among each checklist, especially in the CONSORT for Abstract and ICWM-specific items.

Conclusion
Although CONSORT appears to have enhanced the reporting of RCTs in UC, the quality of ICWM specifics is variable and in need of improvement. Reporting guidelines of the ICWM recommendations should be developed to improve their quality.
The role of Green Public Procurements in energy transition: the case of Western Macedonia
Authors: Kilintzis Pavlos , Samara Elpida , Topaloglou Lefteris , Avlogiaris Giorgos , Kafetzopoulos Dimitrios 
Published: December 2023

AbstractThis paper aims to assess the level of implementation of Green Public Procurements (GPP) in the Region of Western Macedonia—Greece, which is currently under energy transition from lignite to a RES-based energy model. By that assessment, a linkage between the GPP framework and energy transition is created, revealing the distinct GPP criteria that should be adopted by energy-transition regions, which have the potential to strengthen the transition process. Rather than implementing the total number of the GPP criteria, literature and methodology findings indicated that specific GPP criteria are directly connected to energy transition and should be primarily adopted by policymakers in comparison to the rest. These criteria are Electricity, Road Transport, Waste Water Infrastructure and Road Lighting and Traffic Signals. By assessing a sample of 31 procurement officials and scientific experts in the five major municipalities of the region, the implementation level of GPP criteria was measured average-to-low, implying that there is a considerable scope by the region’s main municipal authorities to adopt them to a greater extent. Statistical correlations using Spearman’s rank correlation analysis between specific GPP criteria were also identified, indicating distinct interconnections between the criteria pairs that imply commonalities in the implementation framework of GPP. The paper’s implications for future research led to the identification of specific GPP criteria in the public procurement process, that have the potential to enhance green sustainability and boost energy transition.
Satellite telemetry reveals space use of diamondback terrapins
Authors: Lamont Margaret M. , Price Melissa E. , Catizone Daniel J. 
Published: December 2023

AbstractMovement and space use information of exploited and imperiled coastal species is critical to management and conservation actions. While satellite telemetry has been successfully used to document movements of marine turtles, the large tag sizes available have limited use on smaller turtle species. We used small Argos-based satellite tags to document movement patterns of diamondback terrapins (Malaclemys terrapin), the only estuarine turtle species in North America. Movement data from ten terrapins in St. Joseph Bay, Florida were gathered between July 13, 2018 and July 22, 2021. We estimated seasonal space use using the daily locations generated from a Bayesian hierarchical state-space model to calculate minimum convex polygons (95% MCP) and kernel density estimates (50% and 95% KDE). Mean tracking duration was 125 days and mean home range size was 9.4 km2(95% MCP) and 8.1 km2(95% KDE). Seagrass habitat comprised 55.8% of all home ranges on average, whereas salt marsh comprised a mean of 3.0%. Mean elevation used by terrapins was − 0.13 m (95% MCP) and -0.35 m (95% KDE). Satellite telemetry provided broad-scale spatiotemporal movement and space use data; however, Argos error produced considerable noise relative to true terrapin movements given their size, speed, and behavior. Terrapin home ranges were greater than previously reported and three of the ten terrapins exhibited repeated long-distance, directed movements within the bay. Small patches of salt marsh habitat were centralized within home ranges, despite comprising only a small percentage for each terrapin. Moreover, the percentage of salt marsh present in each core use area was positively correlated with terrapin mass. Although considered an estuarine species, seagrass habitat comprised a large portion of terrapin home ranges; however, our data did not provide the detail necessary to understand how terrapins were using this habitat. As northward-expanding mangroves continue to infringe upon salt marsh habitat, there is potential for negative impacts to terrapin populations across the northern Gulf of Mexico. As salt marsh habitat continues to be infringed upon by northward-expanding mangroves impacts to terrapins across the northern Gulf of Mexico.
Baseline presentation of atypical anorexia nervosa in Singaporean adolescents: a retrospective cohort study
Authors: Chew Chu Shan Elaine , Tay E. Eric , Lie Mei En Hannah Marian , Khaider Khairunisa Binte , Davis Courtney 
Published: December 2023

Abstract
Background
While atypical anorexia nervosa (AAN) has been found to present with significant physical and psychological complications, the presentation of AAN has not been described in a multi-ethnic Singaporean population.

Methods
This retrospective cohort analysis aimed to characterize the baseline presentation of adolescents with anorexia nervosa (AN) (N = 317) and AAN (N = 141) in a Singaporean cohort that presented to a specialist paediatric eating disorder program between January 2010 and October 2020 for assessment.

Results
In patients with AAN, there were increased proportions of males (16% vs. 7%) and of Malay ethnicity (11% vs. 4%) compared to AN. Compared to adolescents with AN, adolescents with AAN had lower rates of admission (61% vs. 81%), bradycardia (45% vs. 75%), and hypotension (7% vs. 21%) but had a higher rate of syncope (13% vs. 7%). Likewise, adolescents with AAN had higher rates of self-harm and drug overdose (14% vs. 1.5%) requiring admission, more purging (45.1% vs. 14.8%) and more shape concerns.

Conclusion
Highlighting the severity of the illness, Singaporean adolescents with AAN presented with physical complications of malnutrition and had more severe eating disorder psychopathology and a higher frequency of other psychological comorbidities than did adolescents with AN.
Factors associated with suicide attempts in the antecedent illness trajectory of bipolar disorder and schizophrenia
Authors: Miola Alessandro , Gardea-Reséndez Manuel , Ortiz-Orendain Javier , Nunez Nicolas A. , Ercis Mete , Coombes Brandon J. , Salgado Manuel Fuentes , Gruhlke Peggy M. , Michel Ian , Bostwick J. Michael , McKean Alastair J. , Ozerdem Aysegul , Frye Mark A. 
Published: December 2023

Abstract
Background
Factors associated with suicide attempts during the antecedent illness trajectory of bipolar disorder (BD) and schizophrenia (SZ) are poorly understood.

Methods
Utilizing the Rochester Epidemiology Project, individuals born after 1985 in Olmsted County, MN, presented with first episode mania (FEM) or psychosis (FEP), subsequently diagnosed with BD or SZ were identified. Patient demographics, suicidal ideation with plan, self-harm, suicide attempts, psychiatric hospitalizations, substance use, and childhood adversities were quantified using the electronic health record. Analyses pooled BD and SZ groups with a transdiagnostic approach given the two diseases were not yet differentiated. Factors associated with suicide attempts were examined using bivariate methods and multivariable logistic regression modeling.

Results
A total of 205 individuals with FEM or FEP (BD = 74, SZ = 131) were included. Suicide attempts were identified in 39 (19%) patients. Those with suicide attempts during antecedent illness trajectory were more likely to be female, victims of domestic violence or bullying behavior, and have higher rates of psychiatric hospitalizations, suicidal ideation with plan and/or self-harm, as well as alcohol, drug, and nicotine use before FEM/FEP onset. Based on multivariable logistic regression, three factors remained independently associated with suicidal attempts: psychiatric hospitalization (OR = 5.84, 95% CI 2.09–16.33, p < 0.001), self-harm (OR = 3.46, 95% CI 1.29–9.30, p = 0.014), and nicotine use (OR = 3.02, 95% CI 1.17–7.76, p = 0.022).

Conclusion
Suicidal attempts were prevalent during the antecedents of BD and SZ and were associated with several risk factors before FEM/FEP. Their clinical recognition could contribute to improve early prediction and prevention of suicide during the antecedent illness trajectory of BD and SZ.
Post-COVID exercise intolerance is associated with capillary alterations and immune dysregulations in skeletal muscles
Authors: Aschman Tom , Wyler Emanuel , Baum Oliver , Hentschel Andreas , Rust Rebekka , Legler Franziska , Preusse Corinna , Meyer-Arndt Lil , Büttnerova Ivana , Förster Alexandra , Cengiz Derya , Alves Luiz Gustavo Teixeira , Schneider Julia , Kedor Claudia , Bellmann-Strobl Judith , Sanchin Aminaa , Goebel Hans-Hilmar , Landthaler Markus , Corman Victor , Roos Andreas , Heppner Frank L. , Radbruch Helena , Paul Friedemann , Scheibenbogen Carmen , Dengler Nora F. , Stenzel Werner 
Published: December 2023

AbstractThe SARS-CoV-2 pandemic not only resulted in millions of acute infections worldwide, but also in many cases of post-infectious syndromes, colloquially referred to as “long COVID”. Due to the heterogeneous nature of symptoms and scarcity of available tissue samples, little is known about the underlying mechanisms. We present an in-depth analysis of skeletal muscle biopsies obtained from eleven patients suffering from enduring fatigue and post-exertional malaise after an infection with SARS-CoV-2. Compared to two independent historical control cohorts, patients with post-COVID exertion intolerance had fewer capillaries, thicker capillary basement membranes and increased numbers of CD169+ macrophages. SARS-CoV-2 RNA could not be detected in the muscle tissues. In addition, complement system related proteins were more abundant in the serum of patients with PCS, matching observations on the transcriptomic level in the muscle tissue. We hypothesize that the initial viral infection may have caused immune-mediated structural changes of the microvasculature, potentially explaining the exercise-dependent fatigue and muscle pain.
Graphical Abstract