bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic predictive value of peripheral blood count and inflammatory markers in gastric cancer
Authors: sun hongyang , Liu Jincheng , Lu Liang 
Published: October 2023

Abstract
ObjectiveTo investigate the correlation between the systemic immuno-inflammatory index (SII), platelet-to-lymphocyte ratio (PLR),monocyte/lymphocyte ratio (MLR) and neutrophil-to-lymphocyte ratio (NLR) and gastric cancer and to evaluate the predictive value of SII, NLR, MLR and PLR for gastric cancer diagnosis.
Methods Clinical data (age, sex, BMI, blood indexes, carcinoembryonic antigen, and alpha-fetoprotein) were retrospectively collected from 318 patients with a primary diagnosis of gastric cancer, gastric polyps or chronic gastritis treated in Chaohu Hospital of Anhui Medical University. The patients were divided into training and validation groups at a ratio of 7:3 by applying the Rand random function. After identifying the indicators with significant intergroup differences, the receiver operator characteristic curves (ROC) were plotted, and the optimal cut-off value, area under the curve (AUC), OR and 95% confidence interval (95% CI) of the independent risk factors for the diagnostic prediction of gastric cancer were analyzed to establish a diagnostic prediction model. This model was applied to the validation group to assess the discriminatory degree of the model.
Results ROC analysis showed that the optimal thresholds for monocyte count, SII, NLR, MLR, PLR, methemoglobin, carcinoembryonic antigen, leukocytes, and lymphocytes were 0.41×109/L, 554.44, 3.14, 0.24, 151.54, 1.43 µg/L, 5.01 µg/L, 4.34×109/L, and 1.68× 109/L, respectively. Univariate analysis showed that sex, age, BMI, monocyte count, SII, NLR, MLR, PLR, alpha-fetoprotein, carcinoembryonic antigen, leukocyte count, and lymphocyte count were associated with gastric cancer. Multivariate analysis showed that the AUC of the diagnostic prediction model was 0.846 (95% CI 0.809-0.884, P < 0.001) in the training group, and when the diagnostic optimal critical value of the training group was applied to the validation group, the model had an AUC of 0.887 (95% CI 0.835-0.939, P < 0.001). These results suggest that the model has good differentiation. The SII, PLR and carcinoembryonic antigen values were lower in gastric cancer patients after treatment than before treatment.
Conclusion SII, NIR, MLR, and PLR are independent risk factors for gastric cancer, and MIR, PLR, alpha-fetoprotein, and carcinoembryonic antigen could be jointly used for the diagnosis and screening of gastric cancer.
Talazoparib enhances the Quinacrine-mediated apoptosis in patient-derived oral mucosa CSCs by inhibiting BER pathway through the modulation of GCN5 and P300
Authors: Das Chinmay , Dash Somya Ranjan , Sinha Saptarshi , Paul Subarno , Das Biswajit , Bhal Subhasmita , Sethy Chinmayee , Kundu Chanakya Nath 
Published: October 2023

Abstract
The presence of cancer stem cells (CSCs) in the tumor microenvironment (TME) is majorly responsible for the development and recurrence of cancer. Earlier reports suggested that upon DNA damage, Poly-(ADP-ribose) Polymerase-1 (PARP-1) helps in chromatin modulation and DNA repair process, thereby promoting CSC survival. But whether a combination of DNA damaging agents along with PARP inhibitors can modulate chromatin assembly, inhibit DNA repair processes, and subsequently target CSCs is not known. Hence, we have investigated the effect of nontoxic bioactive compound quinacrine (QC) and a potent PARP inhibitor Talazoparib in patient-derived oral mucosa CSCs (OM-CSCs) and in vivo xenograft mice preclinical model systems. Data showed that QC + Talazoparib inhibited the PARP-1-mediated chromatin remodelers’ recruitment and deregulated HAT activity of GCN5 (general control nonderepressible-5) and P300 at DNA damage site, thereby preventing the access of repair proteins to the damaged DNA. Additionally, this combination treatment inhibited topoisomerase activity, induced topological stress, and induced apoptosis in OM-CSCs. Similar results were observed in an in vivo xenograft mice model system. Collectively, the data suggested that QC + Talazoparib treatment inhibited BER pathway, induced genomic instability and triggered apoptosis in OM-CSCs through the deregulation of PARP-1-mediated chromatin remodelers (GCN5 and P300) activity.
Fine-mapping and evolutionary history of R-BPMV, a dominant resistance gene to Bean pod mottle virus in Phaseolus vulgaris L.
Authors: MEZIADI Chouaïb , ALVAREZ-DIAZ Juan-Camilo , THAREAU Vincent , GRATIAS Ariane , MARANDE William , SOLER-GARZON Alvaro , MIKLAS Phillip N. , PFLIEGER Stéphanie , Geffroy Valerie 
Published: October 2023

Abstract
Bean pod mottle virus (BPMV) is a comovirus that infects common bean and legumes in general. BPMV is distributed throughout the world and is a major threat on soybean, a closely related species of common bean. In common bean, BAT93 was reported to carry the R-BPMV resistance gene conferring resistance to BPMV and linked with the I resistance gene. To fine map R-BPMV, 182 recombinant inbred lines (RILs) derived from the cross BAT93 x JaloEEP558 were genotyped with polymerase-chain (PCR)-based markers developed using genome assemblies from G19833 and BAT93, as well as BAT93 BAC clone sequences. Analysis of RILs carrying key recombination events positioned R-BPMV to a target region containing at least 16 TIR-NB-LRR (TNL) sequences in BAT93. Because the I cluster presents a suppression of recombination and a large number of repeated sequences, none of the 16 TNLs could be excluded as R-BPMV candidate gene. The evolutionary history of the TNLs for the I cluster were reconstructed using microsynteny and phylogenetic analyses within the legume family. A single I TNL was present in Medicago truncatula and lost in soybean, mirroring the absence of complete BPMV resistance in soybean. Amplification of TNLs in the I cluster predates the divergence of the Phaseolus species, in agreement with the emergence of R-BPMV before the separation of the common bean wild centers of diversity. This analysis provides PCR-based markers useful in marker-assisted selection (MAS) and laid the foundation for cloning of R-BPMV resistance gene in order to transfer the resistance into soybean.
Novel Genetic Variants of HLA Gene Associated with Thai Behcet’s Disease (BD) Patients Using Next Generation Sequencing Technology
Authors: Sornsamdang Gaidganok , Shobana John , Chanprapaph Kumutnart , Chantratita Wasun , Chotewutmontri Sasithorn , Limtong Preeyachat , O-charoen Pichaya , Sukasem Chonlaphat 
Published: October 2023

Abstract
Behcet’s disease (BD) is a recurring inflammatory condition that mostly affects the eyes, mouth, and genitals, with organ involvement in the worst-case scenario. Along with environmental and other patient-related factors, genetic polymorphisms play a huge role in BD. This study aimed to investigate the susceptible genetic variants of HLA gene in 56 Thai BD patients and 192 healthy controls. HLA Genotyping was performed by next-generation sequencing (NGS) using a PacBio kit. The association was calculated by the multivariate logistic regression analysis. We found that the following alleles were found to be associated with BD: HLA-A*26:01:01 (OR=6.02, 95% CI=1.45-22.37, p=0.031), HLA-B*39:01:01 (OR=12.59, 95% CI=1.82-78.50, p=0.005), HLA-B*51:01:01 (OR=3.36, 95% CI=1.18-9.54, p=0.023), HLA-DQB1*05:03:01 (OR=9.35,95%CI=3.72-23.39, p=0.0001), and HLA-DRB1*14:54:01 (OR=32.48, 95% CI=18.11-60.78, p=0.001). When the association analysis was performed for a specific BD phenotype (ocular), HLA-DRB1*14:54:01 (OR=11.67, 95% CI=2.86-47.57, p = 0.001), HLA-B* 51:01:01 (OR=5.47, 95% CI=0.99-90.12, p = 0.05), and HLA-B* 51:01:02 (OR=5.33, 95% CI=1.23-23.06, p = 0.025) alleles had the association with ocular involvement in BD, and the HLA DRB1*14:54:01 (OR=3.35, 95% CI=1-11.99, p = 0.04) allele was found to be linked to vascular involvement in BD patients. In conclusion, for the first time in the Thai population, our study discovered susceptible novel alleles in BD patients such as HLA-A*26:01:01, HLA-B*39:01:01, HLA-B*51:01:01, HLA-DRB1*14:54:01, and HLA-DQB1*05:03:01. This highlights the necessity of genetic testing of the disease's risk alleles to diagnose BD. Despite these alleles have a lower positive predictive value, nearly all the observed risk alleles have significant negative predictive values.
The role of desire over obligation in explaining continued smoking abstinence
Authors: Cox Sharon , Brown Jamie , Perski Olga , Tattan-Birch Harry , Shahab Lion 
Published: October 2023

Abstract
Objective: To assess the role of self-reported desire over obligation in explaining continued smoking abstinence in recent ex-smokers.
Method: A 12-month prospective study of 230 (45.2% female; mean age 45.4 years) adults in England who confirmed they had made a serious quit attempt and were not currently smoking at baseline. The predictor variable was assessed at baseline through responses to the statement: “I am stopping smoking because I want to rather than because I feel I have to” on a 7-point Likert scale (1 = ‘strongly disagree’; 7 = ‘strongly agree’). The outcome was smoking status at 12-month follow-up. Covariates were baseline sociodemographic and smoking-related characteristics. Associations were assessed with logistic regression.
Results: At 12-month follow-up, 59.1% (n=136) reported they were still not smoking and 40.9% (n=94) had restarted smoking. Longer time since quit was positively associated with quit success maintenance at 12-months (adjusted Odds Ratio (AOR) 1.28, 95% Confidence Interval (CI) 1.04-1.58). People who were more strongly motivated to quit by desire were equally or less likely to still be abstinent at 12-months than those who were more motivated by obligation (AOR 0.85, 95%CI 0.70-1.01).
Conclusion: Being motivated by desire does not appear to be positively associated with continued smoking abstinence at 12-month follow-up. By contrast, our findings indicate that obligation may play a role in sustaining quit attempts, but more data across multiple time points are needed to understand this association.
Identifying Barriers and Facilitators to Care for Infants with Bronchopulmonary Dysplasia After NICU Discharge: A Prospective Study of Parents and Clinical Stakeholders
Authors: Lagatta Joanne , Harris Melissa , Cusatis Rachel , Malnory Margaret , Dawson Sara , Konduri Girija 
Published: October 2023

Abstract
Objective Understand barriers and facilitators to follow-up care for infants with bronchopulmonary dysplasia (BPD).Methods Qualitative study of parents and clinical stakeholders caring for infants with BPD. The interview guide was developed by a mother of a former 23-week preterm infant, neonatologist, pulmonologist, nurse, and qualitative researcher. Purposive sampling obtained a heterogenous sociodemographic and professional cohort. Subjects discussed their experience with BPD, barriers to care, caregiver quality of life and health education. Interviews were audio-recorded, transcribed and coded. Thematic analysis was used.Results Eighteen parents and 20 stakeholders completed interviews. Family-level themes included pragmatic barriers like transportation being multi-faceted; and caregiving demands straining mental health. System-level themes included caregiver education needing to balance process needs with future trajectories; and integration of primary care, specialty care, and community supports.Conclusions Individual and system barriers impact follow-up for infants with BPD. This conceptual framework can be used to measure and improve care.
Air quality monitoring device to mitigate the spread of COVID-19 in educational buildings.
Authors: Quiroga Diego , Diaz Sergio , Pastrana Homero F. 
Published: October 2023

Abstract

The COVID-19 pandemic brought significant consequences on healthcare systems, economy and politics. Nowadays, we know that the pathogen responsible for COVID-19 is transmitted mainly by aerosol droplets exhaled by infected individuals, which remain suspended in indoor air. There has been widespread interest in monitoring the CO2 levels in indoor spaces since an infected patient exhales CO2 and infectious aerosols when breathing. So, we designed and built an Air Quality Monitoring Device (AQMD) that measures and analyzes the levels of CO2 and particulate matter in the classrooms of a university with the aim of mitigating the spread of COVID-19. We divided the AQMD design into 2 phases: i) Data measurement and ii) Estimation of infection risk. Specifically, we measured the air quality in 3 classrooms of a university during different types of activities. Using these data, we calculated the recommended CO2 threshold for our classroom setting and estimated the probability of COVID-19 infection of a susceptible person. Our research shows that indoor CO2 concentrations and the probability of COVID-19 infection are influenced mainly by the type of activity and the number of windows open; besides, the number of students does not significantly impact the indoor CO2 concentrations levels because the range of students in the test scenario (18 to 31) was relatively small.
Ammonia is associated with liver-related complications and predicts mortality in acute-on-chronic liver failure patients.
Authors: Thanapirom Kessarin , Treeprasertsuk Sombat , Choudhury Ashok , Verma Nipun , Dhiman Radha Krishan , Mahtab Mamun Al , Devarbhavi Harshad , Shukla Akash , Hamid Saeed Sadiq , Jafri Wasim , Tan Soek Siam , Lee Guan H. , Ghazinyan Hasmik , Sood Ajit , Kim Dong Joon , Eapen C. E. , Tao Han , Yuemin Nan , Dokmeci A. Kadir , Sahu Manoj , Arora Anil , Kumar Ashish , Kumar Ramesh , Prasad V. G. Mohan , Shresta Ananta , Sollano Jose , Payawal Diana Alcantara , Lau George , Sarin Shiv Kumar 
Published: October 2023

Abstract
Background and aims: The relationship between ammonia and liver-related complications (LRCs) in acute-on-chronic liver failure (ACLF) patients is not well defined. This study aimed to evaluate the association of ammonia levels with LRCs in patients with ACLF. The study also evaluated the role of ammonia in predicting mortality and progression of LRCs.
Methods: ACLF patients using the APASL definition were prospectively recruited from the ACLF Research Consortium (AARC) between 2009 and 2019. A total of 3,871 cases were recruited, with most subjects caused by alcohol and HBV infection. Patients with baseline ammonia were enrolled for analysis. LRCs were a composite endpoint of bacterial infection, overt HE, and ascites.
Results: A total of 701 ACLF patients were enrolled. Ammonia levels were significantly higher in patients with LRCs than those without. Ammonia was significantly higher in ACLF patients with overt HE and ascites, but not in those with bacterial infection. Multivariate analysis revealed that AARC score, and ammonia were independently associated with LRCs. Within a month after admission, 350 patients (49.9%) died. Multivariate analysis demonstrated that older age, low haemoglobin, high INR, creatinine, ALT, and ammonia levels were independent predictors of 30-day mortality. Baseline ammonia ≥ 78 µmol/L was independently related to LRCs with an AUROC of 0.74 (p<0.001). Patients who had ammonia ≥ 78 µmol/L faced a higher risk of death within 30 days.
Conclusions: In ACLF patients, baseline arterial ammonia levels are associated with LRCs, mainly overt HE and ascites. Ammonia was also an independent predictor of 30-day mortality.
Self-inflicted ingestion of harmful chemicals in adolescents and adults: Risk factors and characteristics
Authors: Govender Rajen , Tonder Wayne van , Niekerk Ashley van 
Published: October 2023

Abstract

Background: Injury due to ingestion of harmful chemicals has become an area of concern globally. A specific category of such injuries is those that are self-inflicted. Although intentionality for self-infliction may be difficult to determine, key explanatory risks and demographic factors may contribute towards a better understanding of self-inflicted and harmful chemical ingestion injuries.
Methods: This study used secondary data that had been collected on burn injuries due to injection of harmful chemicals in a sample of South Africans from low-income communities close to major metropolitan centres. Logistic regression was used to determine how the risk for self-inflicted ingestion was differentiated from ingestion due to the actions of another person (other-inflicted ingestion) by sex and age cohort of the victim, and the presence of alcohol by examining paraffin ingestion versus that of other chemicals.
Results:The current findings indicate that sex, age cohort, presence of alcohol, and chemicals other than paraffin are key explanatory factors for an increased risk for self-inflicted ingestion of harmful chemicals.
Conclusions: The study empirically confirms the role of several key risk factors in what remains a largely unreported and understudied phenomenon. The findings contribute towards improved safety policies on availability and sale of chemical products and more focussed community interventions for at-risk individuals such as females and young people. It also flags the importance of assessing for alcohol use and alcohol use disorders at hospital admission of self-ingestion injuries.
A comparative analysis of antioxidant and bioactive constituents in micropropagated and wild Lindernia pusilla medicinal herb
Authors: Baro Tikendrajit , Das Debjani , Boro Sibo , Ali Raju , Kour Sharmistha Brahma , Das Sandeep 
Published: October 2023

Abstract
Lindernia pusila is one of the important medicinal herbs which is being used to treat various diseases. The present experiment is aimed to develop an efficient protocol for in vitro mass propagation of L. pusila and to study somaclonal variations in the micro propagated plants using RAPD technique. Surface sterilization of collected explants of L. pusila was performed using 0.1% mercuric chloride and cultured in MS media with different concentrations of growth regulators. Explant surface sterilization was most effective at 2 & 3 minutes of treatment with 0.1% mercuric chloride resulting in 75% of the explant survival. The media MS + 1 mg/L BAP + 0.2 mg/L NAA showed maximum shoot proliferation and multiplication per explant. After hardening of tissue cultured plants, the whole genome of wild and micro propagated plants was isolated using the DNeasy Plant Mini Kit and RAPD assay was performed using 14 different RAPD primers. Among the 14 RAPD primers, 7 primers formed polymorphic DNA bands in tissue-cultured Lindernia pusila plants. Comparatively the tissue cultured plant extract showed higher content of phenols, flavonoids, and anti-oxidant properties than the wild extracts of L. pusilla. In Chromatographic study of gallic acid, the tissue cultured extract showed more gallic acid concentration (42.9mg/g) than the wild sample (10.9mg/g) and the quercetin constituent of tissue cultured plant was higher than the wild extract. The volatile bioactive components found in the wild and tissue cultured extracts were studied using GC-MS analysis.
Analysis of Dynamic Changes and Driving Factors of Small Wetlands Around Chaohu Lake Based on Remote Sensing Images
Authors: Ding WenQing , Zhou Jiaqi , Deng Qing , Miao Haojie , Shi Guangzhi , Jin Jing 
Published: October 2023

Abstract
Small wetlands are widely distributed in urban and rural areas, serving as important water resources and water environment control units, as well as migratory habitats for flora and fauna and sites for biodiversity conservation. However, they are facing threats from climate change and the transformation of the interface between urban and rural spatial dynamics. Based on Gaofen remote sensing images, this study extracted and validated the extraction accuracy of small wetlands and other land use types around Chaohu Lake from 2015 to 2021 using three techniques, namely, random forest (RF), support vector machine (SVM), and maximum likelihood (MLE). Changes in the number of areas of small wetlands and the main driving factors during the period of 6 years were computed using ArcGIS. The results are as follows: (1) The overall classification accuracy and Kappa coefficient trends for 2015, 2018, and 2021 were all RFt > SVM > MLE, and the RF classification effect was the best. (2) The area of small wetlands around Chaohu Lake increased from 9114.42 hm2 in 2015 to 10706.84 hm2 in 2021, but the number decreased from 22279 to 21338. (3) The interaction between two factors has a greater impact on the area of small wetlands than a single factor. The interaction intensity of construction land, annual average precipitation, and altitude is relatively strong with others, which has the strongest impact on the dynamic changes of wetlands. The results emphasize that the accuracy of basic research data on small wetlands can be improved by using high-resolution remote sensing images and selecting classifiers, and that reducing disturbance from anthropogenic construction is a prerequisite for protecting and maintaining the ecological functions of small wetlands, and provide decision-making basis for the sustainable development of small wetlands.
Klebsiella pneumoniae invasive syndrome with liver, lung and brain abscesses complicated with pulmonary fungal infection: a case report and review of the literature
Authors: Luo Yunhao , Hu Wen , Wu Lingna , Duan Shijie , Zhong Xingmei 
Published: October 2023

Abstract
Background: Klebsiella pneumoniae invasion syndrome (KPIS)is a severe multi-site infection that is usually caused by hypervirulent Klebsiella pneumoniae. The bacteria are relatively common in Asian diabetics and can cause organ abscesses or sepsis. When patients develop intracranial infection, the prognosis is poor. After anti-infective treatment, the Klebsiella pneumoniae-induced liver and lung abscessesand pulmonary fungal infection were relieved, but the brain abscess worsened. Such complex and severe infection cases are rarely reported. Early identification of intracranial infection, selection of antibiotics with high concentrations in cerebrospinal fluid, and active treatment of complications such as diabetes and fungal infection are of great significance for the prognosis of patients.
Case presentation: A 71-year-old patient diagnosed with liver abscess in another hospital was transferred to our hospital due to a worsening condition. On day 1 (day of admission), the patient was given invasive mechanical ventilation, continuous renal replacement therapy combined with endotoxin adsorption, antimicrobial treatment with imipenem-cilastatin, and percutaneous catheter drainage for liver abscess. Metagenomic next-generation sequencing in bronchoalveolar lavage fluid indicated Klebsiella pneumoniae, Candida albicans, and Pseudomonas aeruginosa, and no viruses were detected. Blood and pus cultures revealed K. pneumoniae that was sensitive to piperacillin/tazobactam. The anti-infection therapy was adjusted to piperacillin/tazobactam combined with voriconazole.On day 14, a head computed tomography scan showed no significant changes, and a chest CT scan showed absorption of multiple abscesses in both lungs. The patient was still unconscious. After the endotracheal tube was removed, cranial magnetic resonance imaging showed multiple brain abscesses. Finally, his family gave up, and the patient was discharged and died in a local hospital.
Conclusion: In cases of Klebsiella pneumoniae infection, the possibility of intracranial, liver, lung, or other site infections should be considered, and physicians should be vigilant for the occurrence of KPIS. For patients suspected of developing an intracranial infection, cerebrospinal fluid should be tested and cultured as soon as possible, head MRI should be performed, and antibiotics with high distribution in cerebrospinal fluid should be used early. When patients are complicated with diabetes, in addition to glycemic control, vigilance for concurrent fungal infections is also needed.