bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved outcome in HLA-identical sibling hematopoietic stem-cell transplantation for acute myelogenous leukemia predicted by KIR and HLA genotypes
Authors: Hsu Katharine C. , Keever-Taylor Carolyn A. , Wilton Andrew , Pinto Clara , Heller Glenn , Arkun Knarik , O'Reilly Richard J. , Horowitz Mary M. , Dupont Bo 
Published: February 2005

AbstractInhibitory killer immunoglobulin (Ig)-like receptors (KIRs) recognize HLA-C and -B epitopes on target cells, thereby regulating natural killer (NK) cell activity. In 178 patients receiving T-cell-depleted HLA-identical sibling transplants for acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL), or myelodysplastic syndrome (MDS), analysis of donor KIR genotype with HLA genotype demonstrated that 62.9% of the patients lacked an HLA ligand for donor-inhibitory KIR. Lack of HLA ligand for donor-inhibitory KIR (missing KIR ligand) had no effect on disease-free survival (DFS), overall survival (OS), or relapse in patients receiving transplants for CML and ALL. In patients with AML and MDS, however, there was a significant missing KIR ligand effect on DFS (P = .014; hazard ratio [HR], 0.53; 95% confidence interval [95% CI], 0.28-0.88) and OS (P = .03; HR, 0.53; 95% CI, 0.3-0.93). Incidence of relapse was also lower in patients with AML and MDS who lacked the HLA ligand for donor-inhibitory KIR (P = .04; HR, 0.41; 95% CI, 0.18-0.97). AML and MDS patients lacking 2 HLA ligands for donor-inhibitory KIR had the highest DFS (P = .002) and OS (P = .003). There was no significant contribution of donor-activating KIR to transplantation outcome in these patients. These data indicate that the absence of class I ligand in the recipient for donor-inhibitory KIR can be a prognostic factor for transplantation outcome in HLA-identical sibling transplantation and that the lack of HLA-C or -B ligands for donor-inhibitory KIR can contribute to improved outcomes for patients with AML and MDS. (Blood. 2005;105:4878-4884)
A Graves’ Disease-Associated Kozak Sequence Single-Nucleotide Polymorphism Enhances the Efficiency of CD40 Gene Translation: A Case for Translational Pathophysiology
Authors: Jacobson Eric M. , Concepcion Erlinda , Oashi Taiji , Tomer Yaron 
Published: February 2005

AbstractWe analyzed the mechanism by which a Graves’ disease-associated C/T polymorphism in the Kozak sequence of CD40 affects CD40 expression. CD40 expression levels on B cells in individuals with CT and TT genotypes were decreased by 13.3 and 39.4%, respectively, compared with the levels in CC genotypes (P = 0.012). Similarly, Rat-2 fibroblasts transfected with T-allele cDNA expressed 32.2% less CD40 compared with their C-allele-transfected counterparts (P = 0.004). Additionally, an in vitro transcription/translation system showed that the T-allele makes 15.5% less CD40 than the C-allele (P &lt; 0.001), demonstrating that the effect of the single-nucleotide polymorphism (SNP) on CD40 expression is at the level of translation. However, the SNP did not affect transcription, because the mRNA levels of CD40, as measured by quantitative RT-PCR, were independent of genotype. Therefore, our results may suggest that the C allele of the CD40 Kozak SNP, which is associated with Graves’ disease, could predispose to disease by increasing the efficiency of translation of CD40 mRNA.
Synthesis and characteristics of a semi‐interpenetrating polymer network based on chitosan/polyaniline under different pH conditions
Authors: Kim Seon Jeong , Shin Su Ryon , Spinks Geoffrey M. , Kim In Young , Kim Sun I. 
Published: February 2005

AbstractChitosan/polyaniline (PANi) semi‐interpenetrating network (semi‐IPN) polymers were prepared under the condition of HCl aqueous solution (pH < 1). The structure and half oxidation state of emeraldine base PANi, adjusted to pH < 1, underwent changes of structure and electrical properties. Changes of structural properties were investigated using FTIR spectroscopy, solid‐state 13C‐NMR, and wide‐angle X‐ray diffraction. Spectroscopic studies revealed that the PANi was converted to its pH‐switched emeraldine salt form. The surface conductivity of the semi‐IPN depended on the chitosan/PANi weight ratio and the pH condition. The conductivity of the semi‐IPNs increased with increasing PANi content with adjusted pH < 1. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 867–873, 2005
The effect of loading rate on the fracture toughness of fiber reinforced polymer composites
Authors: Jacob George C. , Starbuck J. Michael , Fellers John F. , Simunovic Srdan , Boeman Raymond G. 
Published: February 2005

AbstractThis article is a detailed review of the strain rate dependence of fracture toughness properties in polymer composite materials. An attempt is made to draw together all the strain rate studies done in the past and to elucidate the reasons given by the authors of the reviewed papers for the trends resulting from their studies to better understand the strain rate effects on the fracture toughness of fiber reinforced polymer composite materials. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 899–904, 2005
Preparation and characterization of ultraviolet‐curable nanocomposite coatings initiated by benzophenone/n‐methyl diethanolamine
Authors: Li Fusheng , Zhou Shuxue , Gu Guangxin , You Bo , Wu Limin 
Published: February 2005

AbstractA series of ultraviolet‐curable nanocomposite coatings were prepared with condensed nanosilica particles and with benzophenone/n‐methyl diethanolamine as the initiator. The nanosilica that incorporated into the nanocomposites did not aggregate even when the nanosilica concentration was as high as 22.5%. Adding nanosilica increased the curing speed, thermal stability, and ultraviolet shielding properties of the nanocomposites without reducing the transparency of the ultraviolet‐curing coatings. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 912–918, 2005
DNA transposition – a major contributor to plant chromosome structure
Authors: Flavell R. B. 
Published: February 2005

AbstractHigher plant nuclear genomes contain many families of dispersed repeats that change during evolution. Recent evidence from studies on genetically defined transposable elements raises the possibility that many of the dispersed repeats are remnants of such elements. Transposition of DNA has also occurred between mitochondria, chloroplasts and nuclei, a fact that underlines the major role played by DNA transposition in determining the structure of plant genomes.
Eukaryotic cloning vectors based on bovine papilloma viruses
Authors: DiMaio Daniel 
Published: February 2005

AbstractThe cloning of eukaryotic genes by standard recombinant DNA techniques permits their structural characterization. However, analysis of the expression properties of these genes often requires their introduction into and replication within eukaryotic cells in culture. Certain viral vectors based on the papilloma viruses may prove to be especially important in such investigations. These ‘shuttle vectors’, capable of replication in both bacterial and eukaryotic cells, have already provided several findings of interest about the relationship between eukaryotic gene structure and function. Further analysis of these vectors and their properties promises to enhance their use as investigative tools.
New uses for endothelial cell culture
Authors: Ryan Una S. 
Published: February 2005

AbstractEndothelial cells in culture have, in the past, been a valuable but capricious tool to test hypotheses on the role of components of the blood vessel wall in such functions as blood pressure homeostasis, hemostasis, permeability, transport of macromolecules and the processing of circulating biologically active substances. Now techniques are becoming available for raising long‐term, large‐scale cultures that can be maintained reproducibly and without loss of phenotypic characteristics. The outlook for establishing endothelial cell factories invites speculation on some far‐reaching possibilities, such as endothelial cell banks that could be used for seeding autogenous cells on vascular prostheses, artificial kidney tubing, heart valves, or corneas, or for virus production, or for synthesis by endothelial cells of products at present beyond the reach of bacterial cells.
The influence of alcohol on female germ cells
Authors: Kaufman M. H. 
Published: February 2005

AbstractThe teratogenic effect of ethanol on human and animal embryos is now well documented. Recent studies have clearly demonstrated that ethanol and related spindle‐acting agents may additionally interfere with normal meiotic chromosome segregation during oocyte maturation, leading to the production of aneuploid embryos. The mode of action, and potential hazard posed by these agents is considered.
Membrane tubulin: Fact or fiction?
Authors: Rubin Robert W. 
Published: February 2005

AbstractTubulin is the ubiquitous protein that makes up the walls of the cytoskeletal elements known as microtubules. These 20 nm diameter cylindrical fibers are the spindle fibers for mitosis, provide the skeletal framework for cellular elongation, constitute the major structural and motile elements of cilia and flagella and probably play a number of other roles in eukaryote cells. In the electron microscope, they are never seen to attach or protrude directly into or on cellular membranes. It was therefore with much skepticism that cell biologists responded to recent claims purporting to show that tubulin is (or can be) a membrane protein. This skepticism comes from a bias based on the generally understood function of cytoskeletal proteins as representing the intracellular ‘bones’ of cells. The traditional thinking was that microtubules may attach to cellular membranes but only via a cross‐linking connecting protein. In recent years, however, an increasing number of workers have come to believe that tubulin can exist as a normal membrane protein possibly independent of its role in microtubule function.
Artificial photosynthesis – an example of membrane mimetic chemistry
Authors: Fendler Janos H. 
Published: February 2005

AbstractThe goal of constructing artificial photosynthetic assemblies is to use sunlight for the generation of hydrogen from water; the hydrogen obtained should be an ideal energy source. The use of surfactant vesicle entrapped‐catalyst coated colloidal semiconductors and sacrificial electron donors for photosensitized water reduction illustrates how chemists mimic photosynthesis.
The relationship between human histone gene expression and DNA replication
Authors: Stein Gary S. , Stein Janet L. 
Published: February 2005

AbstractThere is now a wealth of information that histone proteins play a primary role in the structural and transcriptional properties of chromatin, the protein‐DNA complex which constitutes the eukaryotic genome1, 2. In light of the crucial role of histones in cellular function, it is not surprising that their structural genes are found to be controlled in conjunction with the cell cycle, with the synthesis of most histones tightly coupled to nuclear DNA replication. The evidence suggests that this linkage between DNA replication and histone synthesis involves the regulated adjustment of histone mRNA levels through both transcriptional and posttranscriptional control process. The possible mechanisms underlying these diverse control processes are described here.
The molecular genetics of the components of complement and autoimmune diseases
Authors: Porter R. R. 
Published: February 2005

AbstractThe molecular components of complement are a major part of the armoury of the mammalian immune system, being required for the lysis of antibody‐targeted cells. Several of the complement proteins are known to be encoded by genes within the major histocompatibility complex (MHC). Molecular analysis of these genes is providing new information on the basis of complement action and the possible roles of this system in autoimmune disease.
What'S new: The role of chaotropic salts in two‐phase gene diagnosis
Authors: Gillespie David , Caranfa Mary Jo , Bresser Joel 
Published: February 2005

AbstractThe preparation of samples for gene diagnosis is time‐consuming, labor‐intensive and costly. These problems can be overcome by dissolving a biological source in a strong solution of a chaotropic salt, then filtering the solution through an immobilizing membrane. Conditions exist for causing the selective immobilization of either DNA or mRNA. The resultant membrane is immediately ready for reaction with labeled gene probes.
Molecular genetic studies on the thiobacilli and the development of improved biomining bacteria
Authors: Woods David , Rawlings Douglas 
Published: February 2005

AbstractAcidophilic autotrophic thiobacilli, which are able to oxidize metal and solubilize sulphide ores, are used industrially to leach metals from mineral ores. Genetic manipulation of the thiobacilli has the potential for the production of leaching bacteria with desirable characteristics for industry. In this review we examine the development of genetic systems in the thiobacilli and the present status of molecular genetics in the group.
Molecular basis of experimental diabetes: Degeneration, oncogenesis and regeneration of pancreatic B‐cells of islets of Langerhans
Authors: Okamoto Hiroshi 
Published: February 2005

AbstractInsulin is synthesized in pancreatic B‐cells of islets of Langerhans. Understanding the mechanisms of action of B‐cytotoxins on pancreatic islets seems to be important for elucidating not only the causes of diabetes mellitus but also its prevention.
Supercoiled loops and the organization of replication and transcription in eukaryotes
Authors: Zehnbauer Barbara A. , Vogelstein Bert 
Published: February 2005

AbstractThe nuclear DNA of eukaryotes is organized into a series of loops each topologically anchored by elements of the nuclear matrix. Evidence is reviewed which indicates that the anchorage points of the loops are formed by transcriptionally active genes and that individual loops function as replicons. The data suggests a specific model for coupling of DNA replication and transcription in eukaryotes.
This is ICSU: The international union of microbiological societies (IUMS): What is it? what does it do?
Authors: Gerhardt Philipp 
Published: February 2005

AbstractMicrobiological scientists around the world often have only a vague notion about the nature of the international organization with which their national microbiological societies are affiliated. This article provides answers to questions that are usually raised about IUMS; it is reproduced with permission from Microbiological Sciences.
Roots: Cell fusion
Authors: Harris Henry 
Published: February 2005

AbstractThere is a tendency for living things to join up, establish linkage, live inside each other, return to earlier arrangements, get along, whenever possible. This is the way of the world.The new phenomenon of cell fusion, a laboratory trick on which much of today's science of molecular genetics relies for its data, is the simplest and most spectacular symbol of the tendency. In a way, it is the most unbiologic of all phenomena, violating the most fundamental myths of the last century, for it denies the importance of specificity, integrity, and separateness in living things. Any cell ‐ man, animal, fish, fowl, or insect ‐ given the chance and under the right conditions, brought into contact with any other cell, however foreign, will fuse with it. Cytoplasm will flow easily from one to the other, the nuclei will combine, and it will become, for a time anyway, a single cell with two complete, alien genomes, ready to dance, ready to multiply. It is a Chimera, a Griffon, a Sphinx, a Ganesha, a Peruvian God, a Ch'i‐lin, an omen of good fortune, a wish for the world.
Genetic and molecular analyses of Drosophila contractile protein genes
Authors: Fyrberg Eric A. 
Published: February 2005

AbstractTo further comprehend how synthesis and assembly of myofibrillar components is regulated, several laboratories have undertaken genetic studies of muscle development in Drosophila melanogaster. This small fly lends itself well to classical and molecular genetic approaches, and possesses a set of muscle fibers, termed indirect flight muscles (IFM), which is particularly advantageous for such investigations. Structural and functional analyses of cloned Drosophila contractile protein genes have revealed that protein isoforms can be specified either by multigene families or by differentially splicing primary transcripts of a single gene. Characterization of mutations which disrupt flight muscle development has so far revealed that profound abnormalities are caused by defective alleles of actin, tropomyosin, and myosin heavy‐chain genes. Muscle defects associated with particular alleles can be alleviated by integration of corresponding wild‐type gene copies into germ‐line chromosomes. Strategies for further applying genetic techniques to investigations of Drosophila muscle development are discussed.
Spacers and processing of large ribosomal RNAs in Escherichia coli and mouse cells
Authors: Schlessinger D. , Bolla R. I. , Sirdeshmukh R. , Thomas J. R. 
Published: February 2005

AbstractThe formation of mature large rRNAs from larger primary transcripts is very different in bacterial and mammalian cells. In both, cotranscription can help to assure the coordinated production of various rRNA species. However, in bacteria, processing is ordered, initiated by cleavages at double‐stranded stems which enclose the mature sequences; several cleavages are required to produce each mature terminus; and the final steps occur in polysomes, apparently linked to continued protein synthesis. In mouse cells, in contrast, cleavages generate nearly all mature termini stochastically and directly, with no evidence for double‐stranded stems as cleavage signals; and processing occurs in the nucleolus, long before any new rRNA chains arrive in polysomes. Spacer sequences also serve different potnetial functions at the evolutionary extremes. In bacteria, transcribed spacer sequences are associated with different secondary structures in the pre‐rRNA compared to the mature rRNA, and these may promote ribosome formation. In mammalian cells, analyses are too premature to assign any comparable function to transcribed spacer sequences, but the non‐transcribed spacers are probably critical in the organization and replication of nucleoli, functions that are absent from bacteria.
Problems and paradigms: What are the causes of morphogenesis?
Authors: Goodwin B. C. 
Published: February 2005

AbstractProblems and Paradigms is a feature series devoted to discussions of major problems in biology. Past contributions have dealt with the selector gene hypothesis, problems of pattern formation, the phenomenon of paramutation, theories of aging, and the origins of life. In this article, Brian C. Goodwin discusses the problem of morphogenesis, in terms of the idea of the genetic program and in the light of recent findings.
Roots: The origins of molecular genetics: One gene, one enzyme
Authors: Horowitz Norman H. 
Published: February 2005

AbstractRoots presents articles on landmark discoveries that laid the basis for contemporary molecular and cellular biology. In this article, N. H. Horowitz, Professor Emeritus at the California Institute of Technology, and a former associate of George Beadle's, reviews the work that led to the one gene–one enzyme hypothesis.
Non‐enzymatic modification of proteins by steroids: Pathological implications for autoimmunity and glucocorticoid toxicity
Authors: Bucala Richard , Cerami Anthony 
Published: February 2005

AbstractCertain steroids can react with proteins to form stable, covalent addition products. 16α‐hydroxyestrone and the glucocorticoids, for example, contain ketol moieties which permit them to react non‐enzymatically with proteins by forming Schiffbase rearrangement products with lysine residues. The oral contraceptive agent, 17α‐ethinylestradiol, can add to proteins after being enzymatically activated by microsomal oxidation. Elevated plasma levels of these steroids result in an increased amount of protein modification in vivo. Recent investigations have linked the occurrence of steroid‐modified proteins with pathological sequelae associated with high steroid levels.
A biochemical pathway for a cellular behaviour: pHi, phosphorylcreatine shuttles, and sperm motility
Authors: Shapiro Bennett M. , Tombes Robert M. 
Published: February 2005

AbstractSperm motility and respiration are tightly coupled processes, both activated by an increased intracellular pH (pHi). As the sperm pHi increases, the flagellar motor driving motility is activated, leading to ATP consumption. Energy for motility is provided by mitochondrial respiration; energy transport from sperm mitochondrion to tail involves distinct isozymes of creatine kinase that effect a phosphorylcreatine shuttle. The activation of sperm motility and respiration can be described as a linked series of biochemical reactions that form a cell behavioural pathway analogous to the reaction sequences of intermediary metabolism.
Human p53 and human tumours
Authors: Crawford Lionel 
Published: February 2005

AbstractIn normal human cells, a protein with a molecular weight of about 53,000 (p53) is present at a low level, detectable only by sensitive assays. When the cells are infected with some DNA tumour viruses or transformed by the same viruses, the amount of this protein increases dramatically, and much of it is found in a physical association with the virus‐coded protein that causes the transformation. The increase in amount and, incidentally, stability of p53 is not peculiar to virus‐transformed cells, but also occurs in cells transformed by chemicals or radiation where there is no sign of any virus being involved (reviewed by Crawford).1 Cells in culture are not the same as cells in a patient, but these observations do raise questions about the possible involvement of p53 in human tumours.
BUdR, probability and cell variants: Towards a molecular understanding of the decision to differentiate
Authors: Wright Woodring E. 
Published: February 2005

AbstractThe mechanism(s) by which the thymidine analogue 5‐bromodeoxyuridine (BUdR) specifically inhibits the expression of differentiated functions is poorly understood, as are the ways in which cells regulate processes exhibiting probabilistic aspects. I have developed a theoretical model for the regulation of the decision of myogenic cells to differentiate that can explain both of the above phenomena. This model provided a strategy for isolating myoblast variants that had amplified the expression of the factors regulating the decision to differentiate. These myoblasts served as the source of mRNA for making and screening a cDNA library in order to isolate these factors. The successful cloning of these genes should represent a major advance in our understanding of the molecular basis for the major coordinated changes in gene expression that accompany cell differentiation.
A case for human tumor‐suppressor genes
Authors: Stanbridge Eric J. 
Published: February 2005

AbstractMuch of the recent work on oncogenes has been interpreted as signifying that the cancerous phenotype is caused by the direct expression of ‘dominantly‐acting’ oncogenes. On the other hand, numerous somatic cell hybridization experiments suggest that there are potent tumour‐suppressor genes in the genome. The conflict between the observations and the possible nature of the relationships between oncogenes and tumour‐suppressor are discussed.