bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molybdenum‐Copper Antagonism In Metalloenzymes And Anti‐Copper Therapy
Authors: Maiti Biplab K. , Moura Isabel , Moura José J. G. 
Published: January 2024

AbstractThe connection between 3d (Cu) and 4d (Mo) via the “Mo−S−Cu” unit is called Mo−Cu antagonism. Biology offers case studies of such interactions in metalloproteins such as Mo/Cu−CO Dehydrogenases (Mo/Cu−CODH), and Mo/Cu Orange Protein (Mo/Cu−ORP). The CODH significantly maintains the CO level in the atmosphere below the toxic level by converting it to non–toxic CO2 for respiring organisms. Several models were synthesized to understand the structure–function relationship of these native enzymes. However, this interaction was first observed in ruminants, and they convert molybdate (MoO42−) into tetrathiomolybdate (MoS42−; TTM), reacting with cellular Cu to yield biological unavailable Mo/S/Cu cluster, then developing Cu‐deficiency diseases. These findings inspire the use of TTM as a Cu‐sequester drug, especially for treating Cu‐dependent human diseases such as Wilson diseases (WD) and cancer. It is well known that a balanced Cu homeostasis is essential for a wide range of biological processes, but negative consequence leads to cell toxicity. Therefore, this review aims to connect the Mo−Cu antagonism in metalloproteins and anti‐copper therapy.
DMSO‐Related Effects on Ligand‐Binding Properties of Lysine Methyltransferases G9a and SETD8
Authors: Feoli Alessandra , Sarno Giuliana , Castellano Sabrina , Sbardella Gianluca 
Published: January 2024

AbstractBeing the standard solvent for preparing stock solutions of compounds for drug discovery, DMSO is always present in assay buffers in concentrations ranging from 0.1 % to 5 % (v/v). Even at the lowest concentrations, DMSO‐containing solutions can have significant effects on individual proteins and possible pitfalls cannot be eliminated. Herein, we used two protein systems, the lysine methyltransferases G9a/KMT1 C and SETD8/KMT5 A, to study the effects of DMSO on protein stability and on the binding of the corresponding inhibitors, using different biophysical methods such as nano Differential Scanning Fluorimetry (nanoDSF), Differential Scanning Fluorimetry (DSF), microscale thermophoresis (MST), and surface plasmon resonance (SPR), all widely used in drug discovery screening campaigns. We demonstrated that the effects of DMSO are protein‐ and technique‐dependent and cannot be predicted or extrapolated on the basis of previous studies using different proteins and/or different assays. Moreover, we showed that the application of orthogonal biophysical methods can lead to different binding affinity data, thus confirming the importance of using at least two different orthogonal assays in screening campaigns. This variability should be taken into account in the selection and characterization of hit compounds, in order to avoid data misinterpretation.
The BALTO Registry: Long‐term results of percutaneous BALloon pericardioTomy in oncological patients
Authors: Rivero‐Santana Borja , Jimenez‐Valero Santiago , Jurado‐Roman Alfonso , Galeote Guillermo , Lopez‐Fernandez Teresa , Moreno Raul 
Published: January 2024

AbstractObjectivesThe aim of this study was to analyze the efficacy and safety of percutaneous balloon pericardiotomy (PBP) in oncological patients who present with a malignant pericardial effusion (MPE).BackgroundThe use of PBP as a treatment for MPE is not standardized due to the limited evidence. Furthermore, the performance of a second PBP for a recurrence after a first procedure is controversial.MethodsThe BALTO Registry (BALloon pericardioTomy in Oncological patients) is a prospective, single‐center, observational registry that includes consecutive PBP performed for MPE from October 2007 to February 2022. Clinical and procedural, characteristics, as well as clinical outcome were analyzed.ResultsSeventy‐six PBP were performed in 61 patients (65% female). Mean age was of 66.4 ± 11.2 years. In 15 cases, a second PBP procedure was performed due to recurrence despite the first PBP. The procedure could be performed effectively in all cases with only two serious complications. Ninety‐five percent of cases were discharged alive from the hospital. During a median follow‐up of 6.3 months (interquartile range [IQR], 0.9–10.8), MPE recurred in 24.5% cases although no recurrences were reported after the second procedure. No evidence of malignant pleural effusion developed on follow‐up. The median overall survival time was 5.8 months (IQR, 0.8–10.2) and the time to recurrence after the first PBP was 2.4 months (IQR, 0.7–4.5).ConclusionsPBP is a safe and effective treatment for MPE. It could be considered an acceptable therapy in most MPE, even in those who recur after a first procedure.
Synthesis and Characterization of Bola‐Amphiphilic Porphyrin‐Perylenebisimide Architectures
Authors: Schulze Erik J. , Ritterhoff Christian L. , Franz Evanie , Tavlui Olha , Brummel Olaf , Meyer Bernd , Hirsch Andreas 
Published: January 2024

AbstractWe report on the synthesis and characterization of a family of three water‐soluble bola‐amphiphilic zinc‐porphyrin‐perylenebisimide triads containing oligo carboxylic‐acid capped Newkome dendrons in the periphery. Variations of the perylenebisimide (PBI) core geometry and dendron size (G1 and G2 dendrons with 3‐ and 9‐carboxylic acid groups respectively) allow for tuning the supramolecular aggregation behavior with respect to variation of the molecular architecture. The triads show good solubility in basic aqueous media and aggregation to supramolecular assemblies. Theoretical investigations at the DFT level of theory accompanied by electrochemical measurements unravel the geometric and electronic structure of the amphiphiles. UV/Vis and fluorescence titrations with varying amounts of THF demonstrate disaggregation.
Progress of Fluorescent Probes for Protein Phosphorylation and Glycosylation in Atherosclerosis
Authors: Li Jin , Xu Jiheng , Zhao Ruize , Zhang Wei , Li Ping , Zhang Wen , Wang Hui , Tang Bo 
Published: January 2024

AbstractExploring the post‐translational modification (PTM) of proteins in the course of atherosclerotic disease has important guiding significance for the early warning of atherosclerotic plaque, the development of targeted drugs and the treatment of disease. The advancement advanced detection and imaging methods for phosphorylated and glycosylated proteins is an important tool to further reveal the levels of protein phosphorylation and glycosylation during atherosclerotic plaque formation. We present research strategies for detecting protein phosphorylation and glycosylation from the perspective of fluorescent probes, and discuss the feasibility and future direction of the development of these methods for detecting and imaging phosphorylated and glycosylated proteins in atherosclerotic disease.
Synthesis of Interlocked and Non‐Interlocked Deca(para‐phenylene) Derivatives by Ni‐mediated Biaryl Coupling
Authors: Ohta Misuzu , Okuda Ayano , Hosoya Shoichi , Yoshigoe Yusuke , Saito Shinichi 
Published: January 2024

AbstractOligo(para‐phenylene) (PPn) is a compound composed of directly connected 1,4‐phenylene moieties. The synthesis of PPn composed of six or more phenylene moieties with no substituent at the internal phenylene moiety has been challenging because of its low solubility. Herein we synthesized oligo(para‐phenylene)[2]rotaxanes, including a deca(para‐phenylene)[2]rotaxane, with a defined number of phenylene moieties. Biaryl coupling of iodoarenes mediated by macrocyclic dibenzodihydrophenanthroline‐Ni complex was utilized for the first time to synthesize the [2]rotaxanes. Compared to the non‐interlocked deca(para‐phenylene), the deca(para‐phenylene)[2]rotaxane showed higher solubility. The properties of the oligo(para‐phenylene)[2]rotaxanes and non‐interlocked oligo(para‐phenylene)s were analyzed by spectroscopic methods.
Synthesis, Theoretical and Experimental Investigation of Electronic Properties of New Fluoranthenyl‐Based Compounds for OLEDs Applications
Authors: Bennacer PHD. Younes , Guergouri Mounia , Bensegueni Rafik , Boutebdja Mehdi , Kherrouba Abdelmadjid 
Published: January 2024

AbstractThree novel conjugated molecules have been synthesized: 2‐(fluoranthen‐3‐yliminomethyl)‐3,4‐ethylenedioxythio‐phene (EIF), 2‐(fluoranthen‐3‐yliminomethyl) thiophene (TIF), and 2‐(fluoranthen‐3‐yliminomethyl) fluorene (FIF). Optical properties were obtained from electronic absorption and emission spectra in DMSO solution. The solvatochromic properties of the molecules have been studied in different solvents. Electrochemical properties were studied by cyclic voltammetry in a Bu4NBF4 (0.1 M)/MeCN organic solution. As part of investigations to explain the nature of electronic transition process, we have performed geometry optimization of both the ground and the vertical excitation states, using the DFT B3LYP/6‐311G (d, p) and TD‐DFT (CPCM)/B3LYP/6‐311G (d, p) approaches, respectively. Theoretical calculations closely match the experimental findings. Results show that EIF, TIF and FIF are potential candidates to be used as electron transport layer in Organic Light‐Emitting Diodes (OLEDs).
Ultrasound‐Assisted Preparation and Performance Regulation of Electrocatalytic Materials
Authors: Deng Qibo , Chen Shuang , Wu Wenliu , Zhang Shiyu , An Cuihua , Hu Ning , Han Xiaopeng 
Published: January 2024

AbstractWith the advancement of scientific research, the introduction of external physical methods not only adds extra freedom to the design of electro‐catalytical processes for green technologies but also effectively improves the reactivity of materials. Physical methods can adjust the intrinsic activity of materials and modulate the local environment at the solid‐liquid interface. In particular, this approach holds great promise in the field of electrocatalysis. Among them, the ultrasonic waves have shown reasonable control over the preparation of materials and the electrocatalytic process. However, the research on coupling ultrasonic waves and electrocatalysis is still early. The understanding of their mechanisms needs to be more comprehensive and deep enough. Firstly, this article extensively discusses the adhibition of the ultrasonic‐assisted preparation of metal‐based catalysts and their catalytic performance as electrocatalysts. The obtained metal‐based catalysts exhibit improved electrocatalytic performances due to their high surface area and more exposed active sites. Additionally, this article also points out some urgent unresolved issues in the synthesis of materials using ultrasonic waves and the regulation of electrocatalytic performance. Lastly, the challenges and opportunities in this field are discussed, providing new insights for improving the catalytic performance of transition metal‐based electrocatalysts.
Putting eDNA to the test: A field comparison of eDNA metabarcoding to established protocols for assessing biodiversity in Missouri's Ozark Highland streams
Authors: Lee Veronica M. , Berkman Leah K. , Geheber Aaron D. , Landwer Brett , Ludwig Eric J. , Duvernell David D. 
Published: January 2024

AbstractAquatic biodiversity monitoring to inform conservation and management efforts in‐stream systems has increasingly begun to incorporate environmental DNA (eDNA)‐based sampling methods. We conducted a comparison of eDNA metabarcoding to a traditional protocol of combined seining and electrofishing methods to assess fish biodiversity of wadeable stream sites in six separate drainages in the Ozark Highlands of Missouri (USA). The study further focused on the headwaters of the Meramec River, which included 11 sites and seasonal sampling (summer and winter). We compared estimates of diversity across sampling methods, assessed the influences of water flow (depth, velocity, and discharge) and season, and tested the effects of sampling method and site locality on fish assemblage composition. eDNA sampling detected approximately double the number of species compared to traditional methods, providing higher diversity estimates while maintaining the relative ranking of sites. eDNA detection probabilities were positively associated with stream depth and velocity and were generally higher in summer than in winter but not for all species. Estimated species richness was positively associated with discharge for both methods but the relationship was stronger with eDNA sampling. Assemblage differences between tributary and mainstem sites were attributable to a small number of species that were found predominantly in one stream size category or the other, indicating that eDNA was sensitive to within‐drainage assemblage structure relationships. We highlight improved species detection, a more comprehensive understanding of assemblage structural dynamics, and the potential ability to integrate data across sampling methods as important benefits that encourage the use of eDNA metabarcoding as a primary collection method in future stream biodiversity assessment and monitoring programs.
Effect of operating parameters of desiccant wheel on the performance of solar desiccant dehumidifier
Authors: Kushwaha Pravesh Kumar , Kumar Amit , Choudhary Rajesh 
Published: January 2024

AbstractTo produce dry air, desiccant wheel is a very common choice now a day. However, it's crucial to utilize the desiccant wheel effectively. Therefore, in the present investigation, a numerical model is developed to identify the best operating conditions for a solar desiccant dehumidifier based on desiccant wheel and it is observed that the dehumidification capacity can be increased by simultaneously increasing the process air velocity and RPH of the desiccant wheel. A higher temperature of hot air for regeneration allows for a higher process‐to‐regeneration sector facial area ratio, which results in higher amount of dry air. After the mathematical investigation, the desiccant wheel is attached with solar air heater for experimental investigation. The solar air heater is based on both ends open evacuated tube collector, which is able to produce hot air at about 150°C at a mass flow rate of 143 kg/h. At this temperature the desiccant wheel shows remarkable dehumidification effectiveness of 0.61 with an average moisture removal of 0.0098 kgwp/kgda. The average thermal effectiveness and DCOP of the solar desiccant dehumidifier are observed to be 0.24 and 0.23, respectively.
Dynamic cyclic loading tests for investigating the influence of loading rate and axial force on the lateral response of RC columns
Authors: Park Jamin , Park Minseok , Chae Yunbyeong , Kim Chul‐Young 
Published: January 2024

AbstractThe material properties of steel and concrete vary depending on the loading rate to which they are subjected. To realistically evaluate the seismic performance of reinforced concrete (RC) columns, it is necessary to simulate the loading rate that structures will experience during seismic events as accurately as possible. Real‐time dynamic testing is preferred over quasi‐static testing for this purpose. On the other hand, the seismic performance of RC columns also depends on the axial force applied to the column. However, due to the large axial stiffness of RC columns, accurately controlling the axial force during the test, particularly in real‐time testing, is challenging. Consequently, only a few component‐level dynamic tests have been conducted for RC columns. This study aims to explore the influence of axial force and loading rate on the seismic performance of RC columns. To this end, both slow and fast cyclic loading tests were conducted on square cross‐section RC columns subjected to four different axial forces. By utilizing a robust actuator control method along with a specially designed load transfer element, the axial force was successfully controlled, while the lateral displacements were imposed on the columns in real‐time. The results include lateral strengths, postyield responses, damage patterns, rebar strains, and their rates, all of which constitute unique experimental data that can contribute to a deeper understanding of the actual seismic response of RC columns.
On the prevalence of ‘food addiction’ in persons with bulimia nervosa
Authors: Meule Adrian 
Published: January 2024

AbstractA meta‐analysis by Praxedes and colleagues published in this journal reports that the prevalence of ‘food addiction’ as measured with the Yale Food Addiction Scale is lower than 50% in persons with bulimia nervosa and higher in persons with binge eating disorder. However, closely examining the supplementary material of that article reveals that these numbers cannot possibly be correct. Instead, most studies indicate that the prevalence of ‘food addiction’ is higher than 80% in persons with bulimia nervosa and, thus, higher than in persons with other eating disorders.
KDS2010, a reversible MAO‐B inhibitor, extends the lifetime of neural probes by preventing glial scar formation
Authors: Chae Uikyu , Chun Heejung , Lim Jiwoon , Shin Hyogeun , Smith Wesley Charles , Choi Ji Won , Park Ki Duk , Lee C. Justin , Cho Il‐Joo 
Published: January 2024

AbstractImplantable neural probes have been extensively utilized in the fields of neurocircuitry, systems neuroscience, and brain‐computer interface. However, the long‐term functionality of these devices is hampered by the formation of glial scar and astrogliosis at the surface of electrodes. In this study, we administered KDS2010, a recently developed reversible MAO‐B inhibitor, to mice through ad libitum drinking in order to prevent glial scar formation and astrogliosis. The administration of KDS2010 allowed long‐term recordings of neural signals with implantable devices, which remained stable over a period of 6 months and even restored diminished neural signals after probe implantation. KDS2010 effectively prevented the formation of glial scar, which consists of reactive astrocytes and activated microglia around the implant. Furthermore, it restored neural activity by disinhibiting astrocytic MAO‐B dependent tonic GABA inhibition induced by astrogliosis. We suggest that the use of KDS2010 is a promising approach to prevent glial scar formation around the implant, thereby enabling long‐term functionality of neural devices.
The feasibility, acceptability and potential efficacy of a parental text message and social media program on children's vegetable consumption and movement behaviours: A pilot randomised controlled trial
Authors: Norman Jennifer , Furber Susan , Bauman Adrian , Okely Anthony D. 
Published: January 2024

AbstractIssue AddressedAustralian children fall short of meeting the dietary, physical activity and sedentary behaviour guidelines. This study aimed to test the feasibility, acceptability and potential efficacy of a parental text message and social media program on, primarily, their school‐aged children's vegetable consumption and movement behaviours, and, secondarily, their own.MethodsBetween August and November 2022, we conducted a two‐armed randomised controlled trial with 242 parents/caregivers of primary school‐aged children in New South Wales. The ‘Adventure & Veg’ intervention ran for 8 weeks, promoting vegetable eating behaviours, local outdoor physical activity opportunities and ideas for reducing screen time. Feasibility and acceptability were assessed via recruitment and retention data, intervention metrics and self‐reported participant data. Vegetable intake and movement behaviour data were collected via online‐surveys and effect sizes were examined.ResultsMost participants reported that they enjoyed receiving the text messages (88%) and the delivery frequency was acceptable (94%). Limitations to Facebook as a delivery platform were reported. The majority of participants used the text messages to influence the vegetable eating (65%) and movement (77%) behaviours of their child. Significant effects were observed among intervention child participants compared with control for mean daily vegetable consumption (0.45 serves, CI: .19; .71, p = .001, d = .5); weekly vegetable variety (1.85, CI: .25; 3.45, p < .001, d = .6); and weekly physical activity variety (.64 CI: .09; 1.19, p = .022, d = .3). Parents in the intervention group increased their daily vegetable intake by .44 serves (CI: .11; .78, p = .01, d = .4).ConclusionsA parental text message and social media program has potential to support children's vegetable intake and movement behaviours. Further research is required to explore different online delivery methods to promote local outdoor activity options.So What?The Adventure & Veg program holds promise as a stand‐alone health promotion intervention or as a useful adjunct to current family or school‐based healthy lifestyle programs.
Hyperglycemia and elevated C‐reactive protein are independent predictors of hospital mortality in hospitalized COVID‐19 patients in South‐Kivu, eastern Democratic Republic of the Congo: A cross‐sectional study
Authors: Tshongo Christian , Baguma Marius , Mateso Guy‐Quesney , Makali Samuel Lwamushi , Bedha Aline , Mwene‐Batu Pacifique , Mihigo Martine , Nzabara Fabrice , Balola Cordule , Kabuya Pierre , Bapolisi Achille , Masimango Mannix I. , Bahizire Esto , Maheshe‐Balemba Ghislain , Shindano Tony A. , Cirhuza Cikomola 
Published: January 2024

AbstractBackground and AimThe coronavirus disease 2019 (COVID‐19) pandemic was a priority public health problem because of its high mortality rate. This study mainly aimed to determine factors associated with a poor outcome in COVID‐19 hospitalized patients in South‐Kivu, an eastern province of the Democratic Republic of the Congo (DRC).MethodsThis observational study retrospectively evaluated medical records of patients consecutively admitted for probable or confirmed COVID‐19 between May 01 and July 31, 2020 at the Hôpital Provincial Général de Référence de Bukavu (HPGRB), a tertiary hospital located in South‐Kivu. A binary logistic regression model was performed to determine the predictors of mortality.ResultsA total of 157 hospitalized COVID‐19 patients aged 57.7 (13.2) years were included in this study. Male gender (69.4%), older age (52.9%), medical history of diabetes (38.2%), and arterial hypertension (35.1%) were the most frequent risk factors. Most patients presented with fever (73.3%), cough (72.6%), and dyspnea (66.2%). Overall, 45.1% of patients died. Intrahospital mortality was significantly associated with advanced age [odds ratio, OR (95% confidence interval, CI) = 2.34 (1.06–5.38)], hypoxemia [OR (95% CI) = 4.67 (2.02–10.77)], hyperglycemia [OR (95% CI) = 2.14 (1.06–4.31)], kidney failure [OR (95% CI) = 2.82 (1.4–5.68)], hyperleukocytosis [OR (95% CI) = 3.33 (1.67–6.66)], and higher C‐reactive protein (CRP) levels [OR (95% CI) = 3.93 (1.93–8.01)]. After adjustment for various covariates, only higher CRP levels [OR (95% CI) = 3.23 (1.23–8.5)] and hyperglycemia [OR (95% CI) = 2.5 (1.02–6.11)] at admission were independently associated with mortality.ConclusionHyperglycemia and marked inflammatory syndrome were the major predictors of poor outcomes in patients hospitalized for COVID‐19 in South‐Kivu. These two factors should be quantified at hospital admission to establish the patient's prognosis.