bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digitalization and the environment: The role of information and communication technology and environmental taxes in European countries
Authors: Adeshola Ibrahim , Usman Ojonugwa , Agoyi Mary , Awosusi Abraham Ayobamiji , Adebayo Tomiwa Sunday 
Published: October 2023

AbstractThis study examines the impacts of digitalization through information and communication technology (ICT) and environmental taxes on greenhouse gas (GHG) emissions in 23 European Union (EU) countries between 2000 and 2017. Using the Pooled Mean Group estimator, the empirical results provide evidence that ICT development and environmental taxes improve environmental sustainability while research and development investments and income per capita deteriorate environmental sustainability. Furthermore, the results based on the Dynamic Panel Threshold Regression model show that the relationship between ICT and GHG emissions is dependent on the level of environmental taxes. During the period of low environmental taxes, the effect of ICT on GHG emissions is positive and insignificant but once environmental taxes cross the threshold value, the effect of ICT becomes negatively related to greenhouse gas emissions. This suggests that the period of low environmental taxes does not support the environmental friendliness of ICT development in the European region. The policy implication of these findings is that ICT, environmental taxes, and renewable energy can be possibly stirred up to achieve long‐term environmental sustainability in the EU region.
The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation, and copy number in normal and abnormal human ejaculates
Authors: Tímermans Ana , Otero Fátima , Garrido Manuel , Gosálvez Jaime , Johnston Stephen , Fernández José Luis 
Published: October 2023

AbstractBackgroundWhile it is common to clinically evaluate sperm nuclear DNA fragmentation, less attention has been given to sperm mitochondrial DNA. Recently, a digital PCR assay has allowed accurate estimation of the proportion of fragmented mtDNA molecules and relative copy number.ObjectivesTo determine the correlation of classical sperm parameters, average mtDNA copies per spermatozoon and the level of mtDNA fragmentation (SDF‐mtDNA) to that of nuclear DNA fragmentation (SDF‐nDNA), measured as the proportion of global, single‐strand DNA (SDF‐SSBs) and double‐strand DNA breaks (SDF‐DSBs). To determine whether the level of nuclear and mitochondrial DNA fragmentation and/or copy number can differentiate normozoospermic from non‐normozoospermic samples.Materials and methodsEjaculates from 29 normozoospermic and 43 non‐normozoospermic were evaluated. SDF was determined using the sperm chromatin dispersion assay. mtDNA copy number and SDF‐mtDNA were analyzed using digital PCR assays.ResultsRelative mtDNA copy increased as sperm concentration or motility decreased, or abnormal morphology increased. Unlike SDF‐mtDNA, mtDNA copy number was not correlated with SDF‐nDNA. SDF‐mtDNA increased as the concentration or proportion of non‐vital sperm increased; the higher the mtDNA copy number, the lower the level of fragmentation. Non‐normozoospermic samples showed double the level of SDF‐nDNA compared to normozoospermic (median 25.00 vs. 13.67). mtDNA copy number per spermatozoon was 3× higher in non‐normozoospermic ejaculates (median 16.06 vs. 4.99). Although logistic regression revealed SDF‐Global and mtDNA copy number as independent risk factors for non‐normozoospermia, when SDF‐Global and mtDNA copy number were combined, ROC curve analysis resulted in an even stronger discriminatory ability for predicting the probability of non‐normozoospermia (AUC = 0.85, 95% CI 0.76–0.94, p < 0.001).ConclusionHigh‐quality ejaculates show lower nuclear SDF and retain less mtDNA copies, with approximately half of them fragmented, so that the absolute number of non‐fragmented mtDNA molecules per spermatozoon is extremely low.
Deconstructing self‐fulfilling outcome measures in infertility treatment
Authors: Mertens Mayli , Mertes Heidi 
Published: October 2023

AbstractThe typical outcome measure in infertility treatment is the (cumulative) healthy live birth rate per patient or per cycle. This means that those who end the treatment trajectory with a healthy baby in their arms are considered to be successful and those who do not are considered to have failed. In this article, we argue that by adopting the healthy live birth standard as the outcome measure that defines a successful fertility treatment, it becomes an interpretative self‐fulfilling prophecy: those who achieve the goal consider themselves successful and those who do not consider themselves failures. This is regardless of the fact that having children is only one out of many ways to alleviate the suffering related to infertility and that stopping fertility treatment can also be a positive decision to move on to other goals, rather than a form of “giving up,” “dropping out,” “nonadherence,” or failure. We suggest that those seeking fertility treatment would be served better by an alternative outcome measure, which can be equally self‐fulfilling, according to which a successful treatment is one in which people leave the clinic released from the suffering that accompanied their status as infertile when they first entered the clinic. This new outcome measure still implies that walking out with a healthy baby is a positive outcome. What changes is that walking out without a baby can also be a positive outcome, rather than being marked exclusively as a failure.
Caregiver skills training for caregivers of individuals with neurodevelopmental disorders: A systematic review and meta‐analysis
Authors: Reichow Brian , Kogan Cary , Barbui Corrado , Maggin Daniel , Salomone Erica , Smith Isaac C. , Yasamy M. Taghi , Servili Chiara 
Published: October 2023

AbstractAimTo systematically review the effectiveness of caregiver and parent skills training programs, including caregiver‐mediated interventions, for caregivers of individuals with neurodevelopmental disorders.MethodWe conducted a systematic review with a random‐effects meta‐analysis. We searched 11 electronic databases through July 2021 and used a snowball methodology to locate relevant articles of randomized controlled trials. Effect size estimates were pooled using Hedges' g from data extracted from study reports and through author requests using random‐effects meta‐analyses for three child outcome categories (child development, adaptive behavior, and problem behavior) and three caregiver outcome categories (parenting skills and knowledge, psychological well‐being, and interpersonal family relations).ResultsWe located 44 910 records, from which 75 randomized controlled trials involving 4746 individuals with neurodevelopmental disorders and their caregivers were included. Random‐effects meta‐analyses showed improvements in child development (g = 0.30; 99% confidence interval [CI] = 0.07–0.53) and reduction in reported problem behaviors (g = 0.41; 99% CI = 0.24–0.59), but not a statistically significant improvement in adaptive behavior (g = 0.28; 99% CI = −0.42 to 0.98). Caregivers showed improvements in parenting skills and knowledge (g = 0.72; 99% CI = 0.53–0.90), psychological well‐being (g = 0.52; 99% CI = 0.34–0.71), and interpersonal family relations (g = 0.76; 99% CI = 0.32–1.20).InterpretationCaregiver skills training programs benefit both caregivers and children with neurodevelopmental disorders. Skills training programs improve child development and behavior, improve parenting skills, reduce caregiver mental health issues, and improve family functioning. Programs using culturally appropriate training material to improve the development, functioning, and participation of children within families and communities should be considered when caring for children with neurodevelopmental disorders.What this paper adds

Caregiver skills training programs are effective interventions for both caregivers and children.
Children with neurodevelopmental disorders benefit from improvements in development and reduction of problematic behaviors.
Caregivers benefit from enhanced skills and psychological well‐being.
Improvements in interpersonal family relationships have also been documented.
Trends in Design and Government in Europe
Authors: Whicher Anna , Swiatek Piotr , Thurston Paul 
Published: October 2023

Encouraged by the Bureau of European Design Associations, the European Union and its legislative arm have launched an Action Plan for Design‐Driven Innovation.In Europe, design is serious business. Nearly half the members of the European Union include design in their national innovation policies, and the European Commission (the EU's executive body) is funding and carrying out an action plan for design.
Oyster reefs' control of carbonate chemistry—Implications for oyster reef restoration in estuaries subject to coastal ocean acidification
Authors: Tomasetti Stephen J. , Doall Michael H. , Hallinan Brendan D. , Kraemer Jeffrey R. , Gobler Christopher J. 
Published: October 2023

AbstractGlobally, oyster reef restoration is one of the most widely applied coastal restoration interventions. While reefs are focal points of processes tightly linked to the carbonate system such as shell formation and respiration, how these processes alter reef carbonate chemistry relative to the surrounding seawater is unclear. Moreover, coastal systems are increasingly impacted by coastal acidification, which may affect reef carbonate chemistry. Here, we characterized the growth of multiple constructed reefs as well as summer variations in pH and carbonate chemistry of reef‐influenced seawater (in the middle of reefs) and ambient seawater (at locations ~50 m outside of reefs) to determine how reef chemistry was altered by the reef community and, in turn, impacts resident oysters. High frequency monitoring across three subtidal constructed reefs revealed reductions of daily mean and minimum pH (by 0.05–0.07 and 0.07–0.12 units, respectively) in seawater overlying reefs relative to ambient seawater (p < .0001). The proportion of pH measurements below 7.5, a threshold shown to negatively impact post‐larval oysters, were 1.8×–5.2× higher in reef seawater relative to ambient seawater. Most reef seawater samples (83%) were reduced in total alkalinity relative to ambient seawater samples, suggesting community calcification was a key driver of modified carbonate chemistry. The net metabolic influence of the reef community resulted in reductions of CaCO3 saturation state in 78% of discrete samples, and juvenile oysters placed on reefs exhibited slower shell growth (p < .05) compared to oysters placed outside of reefs. While differences in survival were not detected, reef oysters may benefit from enhanced survival or recruitment at the cost of slowed growth rates. Nevertheless, subtidal restored reef communities modified seawater carbonate chemistry in ways that likely increased oyster vulnerability to acidification, suggesting that carbonate chemistry dynamics warrant consideration when determining site suitability for oyster restoration, particularly under continued climate change.
Attitudes towards vaccination among parents of children aged 0–5 years: A scale development study
Authors: Doğan Emel , Genç Fatma Zehra , Kaya Emine , Bilgili Naile , Kitiş Yeter , Kan Adnan 
Published: October 2023

AbstractBackgroundVaccination is the most important public health practice for preventing infectious diseases and maintaining health. However, some parents are hesitant to vaccinate their children.DesignThis study was designed as an instrument development study.MethodsThis study aimed to develop a scale to measure the attitudes of parents with children aged 0–5 years towards vaccination and to evaluate the validity and reliability of this scale. The data were collected from 691 participants between November 2020 and January 2021. During the development of the scale, experts provided feedback on its technical, content and language dimensions. The data were analysed using exploratory and confirmatory factor analyses for conceptual validity and Cronbach's α and item‐level test–retest analyses for reliability.ResultsThe developed scale is a reliable and valid tool for measuring attitudes towards vaccination among parents (Cronbach's α = 0.97, variance explained by these 28‐item two factors = 62.47%).ConclusionThis scale can be used as a guide in measuring the attitudes of parents towards vaccination and determining the direction of primary health‐care services based on the results of the measurement.
The effect of weight loss on recurrence of atrial fibrillation after catheter ablation: A systematic review and meta‐analysis
Authors: Akhtar Khawaja Hassan , Jafry Ali Haider , Beard Christopher , Nasir Yusra Minahil , Agarwal Siddharth , Khan Jehanzeb , Clifton Shari , Reece Jessica , Munir Muhammad Bilal , Deshmukh Abhishek , DeSimone Christopher V. , Jackman Warren M. , Stavrakis Stavros , Po Sunny , Sanders Prashanthan , Asad Zain Ul Abideen 
Published: October 2023

AbstractBackgroundObesity is associated with an increased risk of developing recurrent atrial fibrillation (AF) after catheter ablation (CA). However, the current data on weight loss interventions show inconsistent results in preventing the recurrence of AF after CA.MethodsWe conducted a systematic search in MEDLINE and EMBASE to identify studies that reported the outcome of recurrence of AF after CA in obese patients undergoing weight interventions. The subgroup analysis included: (1) Weight loss versus no weight loss, (2) >10% weight loss versus <10% weight loss, (3) <10% weight loss versus no weight loss, (4) Follow‐up <12 months, and (5) Follow‐up >12 months after CA. Mantel–Haenszel risk ratios with a 95% confidence interval (CI) were calculated using a random effects model and for heterogeneity, I2 statistics were reported.ResultsA total of 10 studies (one randomized controlled trial and nine observational studies) comprising 1851 patients were included. The recurrence of AF was numerically reduced in the weight loss group (34.5%) versus no weight loss group (58.2%), but no statistically significant difference was observed (risk ratio [RR] = 0.76; 95% CI: 0.49–1.18, p = .22). However, there was a statistically significant reduction in recurrence of AF with weight loss versus no weight loss at follow‐up >12 months after CA (RR = 0.47; 95% CI: 0.32–0.68, p < .0001). At follow‐up >12 months after CA, both >10% weight loss versus <10% weight loss (RR = 0.49; 95% CI: 0.31–0.80, p = .004) and <10% weight loss versus no weight loss (RR = 0.39; 95% CI: 0.31–0.49, p < .00001) were associated with a statistically significant reduction in recurrent AF.ConclusionIn patients with AF undergoing CA, weight loss is associated with reducing recurrent AF at > 12 months after ablation and these benefits are consistently seen with both >10% and <10% weight loss. The benefits of weight loss in preventing recurrent AF after CA should be examined in larger studies with extended follow‐up duration.
Striking a growth–defense balance: Stress regulators that function in maize development
Authors: Xie Shiyi , Luo Hongbing , Huang Wei , Jin Weiwei , Dong Zhaobin 
Published: October 2023

ABSTRACTMaize (Zea mays) cultivation is strongly affected by both abiotic and biotic stress, leading to reduced growth and productivity. It has recently become clear that regulators of plant stress responses, including the phytohormones abscisic acid (ABA), ethylene (ET), and jasmonic acid (JA), together with reactive oxygen species (ROS), shape plant growth and development. Beyond their well established functions in stress responses, these molecules play crucial roles in balancing growth and defense, which must be finely tuned to achieve high yields in crops while maintaining some level of defense. In this review, we provide an in‐depth analysis of recent research on the developmental functions of stress regulators, focusing specifically on maize. By unraveling the contributions of these regulators to maize development, we present new avenues for enhancing maize cultivation and growth while highlighting the potential risks associated with manipulating stress regulators to enhance grain yields in the face of environmental challenges.
Strain‐dependent role of copper in prion disease through binding to histidine residues in the N‐terminal domain of prion protein
Authors: Hara Hideyuki , Miyata Hironori , Chida Junji , Sakaguchi Suehiro 
Published: October 2023

AbstractThe cellular prion protein, PrPC, is a copper‐binding protein abundantly expressed in the brain, particularly by neurons, and its conformational conversion into the amyloidogenic isoform, PrPSc, plays a key pathogenic role in prion diseases. However, the role of copper binding to PrPC in prion diseases remains unclear. Here, we fed mice with a low‐copper or regular diet and intracerebrally inoculated them with two different mouse‐adapted RML scrapie and BSE prions. Mice with a low‐copper diet developed disease significantly but only slightly later than those with a regular diet after inoculation with BSE prions, but not with RML prions, suggesting that copper could play a minor role in BSE prion pathogenesis, but not in RML prion pathogenesis. We then generated two lines of transgenic mice expressing mouse PrP with copper‐binding histidine (His) residues in the N‐terminal domain replaced with alanine residues, termed TgPrP(5H > A)‐7342/Prnp0/0 and TgPrP(5H > A)‐7524/Prnp0/0 mice, and similarly inoculated RML and BSE prions into them. Due to 2‐fold higher expression of PrP(5H > A) than PrPC in wild‐type (WT) mice, TgPrP(5H > A)‐7524/Prnp0/0 mice were highly susceptible to these prions, compared to WT mice. However, TgPrP(5H > A)‐7342/Prnp0/0 mice, which express PrP(5H > A) 1.2‐fold as high as PrPC in WT mice, succumbed to disease slightly, but not significantly, later than WT mice after inoculation with RML prions, but significantly so after inoculation with BSE prions. Subsequent secondary inoculation experiments revealed that amino acid sequence differences between PrP(5H > A) and WT PrPSc created no prion transmission barrier to BSE prions. These results suggest that copper‐binding His residues in PrPC are dispensable for RML prion pathogenesis but have a minor effect on BSE prion pathogenesis. Taken together, our current results suggest that copper could have a minor effect on prion pathogenesis in a strain‐dependent manner through binding to His residues in the N‐terminal domain of PrPC.image
Melatonin prevents EAAC1 deletion‐induced retinal ganglion cell degeneration by inhibiting apoptosis and senescence
Authors: Hu Chenyang , Feng Yanlin , Huang Guangyi , Cui Kaixuan , Fan Matthew , Xiang Wu , Shi Yuxun , Ye Dan , Ye Huiwen , Bai Xue , Xu Fan , Xu Yue , Huang Jingjing 
Published: October 2023

AbstractNormal tension glaucoma (NTG) is referred to as a progressive degenerative disorder of the retinal ganglion cells (RGCs), resulting in nonreversible visual defects, despite intraocular pressure levels within the statistically normal range. Current therapeutic strategies for NTG yield limited benefits. Excitatory amino acid carrier 1 (EAAC1) knockout (EAAC1−/−) in mice has been shown to induce RGC degeneration without elevating intraocular pressure, mimicking pathological characteristics of NTG. In this study, we explored whether daily oral administration of melatonin could block RGCs loss and prevent retinal morphology and function defects associated with EAAC1 deletion. We also explored the molecular mechanisms underlying EAAC1 deletion‐induced RGC degeneration and the neuroprotective effects of melatonin. Our RNA sequencing and in vivo data indicated EAAC1 deletion caused elevated oxidative stress, activation of apoptosis and cellular senescence pathways, and neuroinflammation in RGCs. However, melatonin administration efficiently prevented these detrimental effects. Furthermore, we investigated the potential role of apoptosis‐ and senescence‐related redox‐sensitive factors in EAAC1 deletion‐induced RGCs degeneration and the neuroprotective effects of melatonin administration. We observed remarkable upregulation of p53, whereas NRF2 and Sirt1 expression were significantly decreased in EAAC1−/− mice, which were prevented by melatonin treatment, suggesting that melatonin exerted its neuroprotective effects possibly through modulating NRF2/p53/Sirt1 redox‐sensitive signaling pathways. Overall, our study provided a solid foundation for the application of melatonin in the management of NTG.
Stability and failure rate during 3 years of fixed retention: A follow‐up of an randomized clinical trial on adolescents with four different lingual retainers
Authors: Çokakoğlu Serpil , Adanur‐Atmaca Rabia , Çakır Merve , Öztürk Fırat 
Published: October 2023

AbstractObjectiveTo evaluate stability outcomes and failure rates associated with four types of lingual retainers: (1) dead‐soft wire, (2) multistrand stainless steel (SS) wire, (3) CAD/CAM nitinol, and (4) connected bonding pads (CBPs) after 3 years of retention.MethodsThis study enrolled 96 patients (66 females, 30 males) with a median age of 19 years with four types of lingual retainers: (1) 0.016 × 0.022‐inch dead‐soft wire, (2) 0.0215‐inch five‐strand SS wire, (3) 0.014 × 0.014‐inch CAD/CAM nitinol wire, and (4) CBPs. The irregularity index, intercanine distances, and arch lengths were obtained and used to evaluate mandibular stability. Failure rates were also assessed during this study. Data were statistically analysed.ResultsIrregularity increased, whereas intercanine width and arch length decreased after 3 years of retention. The greatest irregularity was associated with the CBPs and the least with the CAD/CAM retainers. Changes in stability measurements were significantly higher in the dead‐soft wire and CBPs than those in the CAD/CAM nitinol and multistrand SS wires. Parallel to these changes, the frequency of failure yielded similar results with the same significance between the groups. The failure rate of CBPs, in contrast to the CAD/CAM nitinol and multistrand SS wires, was significantly higher in the right quadrant (P < .05).ConclusionAfter taking the 3‐year results into consideration, CAD/CAM nitinol and multistrand SS wires were found to be more successful than the others in maintaining mandibular stability. The most failures were observed with CBPs after 3 years of retention.
A NARRATIVE TURN: HUMAN AGENCY IN ROCK CARVINGS AT NÄMFORSEN, NORTHERN SWEDEN
Authors: Skoglund Peter , Ranta Michael , Persson Tomas , Rédei Anna Cabak , Gjerde Jan Magne 
Published: October 2023

SummaryThe idea to create pictorial narratives seems to have occurred long after humans learned to produce iconic images, that is, depictions based on visual similarity to external objects. In Scandinavia, e.g. in Gärde, Sweden or Stykket and Bøla, Norway, early Mesolithic images (e.g. rock carvings from before c.5000 BCE) often feature animals that are solitary or without suggestion of causal or narrative relations to other figures, although they sometimes are grouped by proximity or are superimposed on each other. Notable is also the almost complete absence of human figures. However, in subsequent Neolithic configurations (after c.5000 BCE), there are renderings of human agents involved in various forms of interaction with each other or with animals, often within distinguishable ‘scenes’ or compositions.In this paper we outline the emergence of pictorial narratives in Scandinavian rock carvings. In particular, we focus on some of the first depictions of human agency as displayed in Nämforsen, Sweden (c.5000–1800 BCE). Further, we present an analysis of occurring human‐animal relationships, interpretable as early attempts to create narrativizing images.