bluuebunny/crossref_metadata_2025_split huggingface dataset:
A topographical atlas of α-synuclein cell type-specific expression in adult mouse brain and peripheral organs
Authors: Rousseaux Maxime W C , Geertsma Haley , Fisk Zoe , Sauline Lillian , Prigent Alice , Kurgat Kevin , Callaghan Steve , Tomlinson Julianna , Henderson Michael 
Published: November 2023

Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide and presents pathologically with Lewy pathology and dopaminergic neuron loss. Lewy pathology contains aggregated ⍺Synuclein (⍺Syn), a protein encoded by the SNCA gene which is also mutated or duplicated in a subset of familial PD cases. Due to its predominant presynaptic localization, immunostaining for the protein results in diffuse parenchymal reactivity, providing little insight into the types of cells expressing ⍺Syn. As a result, insight into ⍺Syn expression driven cellular vulnerability has been difficult to ascertain. Using a combination of knock-in mice that target ⍺Syn to the nucleus of cells (SncaNLS) and in situ hybridization of Snca in wild-type mice, we systematically mapped the topography and cell types expressing ⍺Syn in the mouse brain, spinal cord, retina, and gut. We find a high degree of correlation between ⍺Syn protein and RNA levels across multiple brain regions and further identify cell types with low and high ⍺Syn content. As expected, we found that ⍺Syn is highly expressed in neurons, particularly those involved in PD and to a lower extent in non-neuronal cell types, notably those of oligodendrocyte lineage, which are relevant to the pathogenesis of multiple system atrophy. Surprisingly, we also find that ⍺Syn is relatively absent from select neuronal clusters, e.g., ChAT-positive motor neurons, whereas enteric neurons universally express some degree of ⍺Syn. We also present a web-based platform allowing for user-friendly interrogation of these results. Taken together, this integrated atlas provides much-needed insight into the cellular topography of ⍺Syn, and provides a quantitative map to test hypotheses about the role of ⍺Syn in network vulnerability, and thus serves investigations into PD pathogenesis and other αSynucleinopathies.
From Fields to Photovoltaics: Effects of Agrisolar Co-Location on Food, Energy, Water, and Economic Security
Authors: Stid Jacob , Shukla Siddharth , Kendall Anthony , Anctil Annick , Hyndman David , Rapp Jeremy , Anex Robert 
Published: November 2023

Abstract
Achieving sustainable net-zero energy production requires understanding how solar PV installations affect the landscape and its critical resources. To enhance this understanding, we investigate agrisolar co-location, which is land that is broadly co-managed for agricultural production, solar photovoltaic electricity production, and ecosystem services. We present a food, energy, water, and economic impact analysis of agricultural output offset by agrisolar co-location for 925 arrays (2.53 GWp covering 39.3 km2) spanning the California Central Valley. We find that agrisolar co-location displaces a significant amount of food production. However, agrisolar co-location also increases economic security for farmers and likely enhances water sustainability, and thus future agricultural production, by reducing irrigation water use and facilitating the fallowing of irrigated land. Given the unprecedented pace of solar PV expansion globally, these results highlight the need for a deeper understanding of the multifaceted outcomes of agricultural and solar PV co-location decisions.
ACSL3 regulates CRC cell proliferation through ACSL3-LPIAT1 signaling pathway
Authors: Chen Jianlin , Liu Xianming 
Published: November 2023

Abstract

Background: Colorectal cancer (CRC) is a common cancer in China, with a high mortality rate. Lipid metabolism disorders can promote cancer metabolism reprogramming, and lipid metabolism related genes are prognostic biomarkers of cancer. Objective: To investigate whether ACSL3 is involved in EMT and metastasis of CRC, and to explore the molecular mechanism of ACSL3 involvement in CRC.
Method: Establish CRC models with high expression of ACSL3 and knockdown of ACSL3. CCK8, flow cytometry, scratch assay, and Transwell assay were used to measure the effect of ACSL3 on CRC cells. In vivo experimental analysis of changes in tumor volume and weight caused by overexpression or knockdown of ACSL3. In addition, we also studied whether ACSL3 affects the change of Prostaglandin through participating in ACSL3-LPAIAT1 signal axis, and then affects the occurrence and progression of tumors.
Result: The apoptosis rate of cell lines with high expression of ACSL3 is significantly reduced. Overexpression of ACSL3 significantly enhances the migration and invasion ability of CRC cells. Silencing ACSL3 can partially reverse the increased invasiveness caused by overexpression of ACSL3. Overexpression of ACSL3 can increase the expression levels of LPIAT1 and cPLA2, which can be partially reversed by silencing ACSL3. In vivo experiments have also found that upregulation of ACSL3 has the effect of accelerating tumor volume and weight.
Conclusion: ACSL3 plays an important role in the progression of CRC, and may be a prognostic biomarker, becoming a new potential therapeutic target for the treatment of CRC.
Performance Evaluation of Hybrid ALOHA Mechanism based on Game Theory and Markov Chain Modeling
Authors: Bellouch Abdessamad , Zaaloul Abdellah , Haqiq Abdelkrim 
Published: November 2023

Abstract

Hybrid ALOHA is a novel Medium Access Control (MAC) protocol that aims to enhance energy efficiency and packet delivery performance in wireless communication networks. This paper leverages mathematical models and analysis techniques from game theory and Markov chain modeling to investigate the performance of the hybrid ALOHA protocol under cooperative and non-cooperative scenarios. We first evaluate the protocol in a team problem, where all users act cooperatively to optimize the global utility of the system, and then in a non-cooperative game, where each user acts in their own self-interest and aims to access the channel by utilizing a higher retransmission probability, which they believe will optimize their own benefits. The use of Markov chain analysis enables the derivation of analytical expressions for the performance metrics of interest, as well as the determination of optimal and equilibrium retransmission probabilities. Additionally, the game theory approach is used to analyze the distribution of retransmission probabilities in the hybrid ALOHA protocol. Throughout an extensive numerical analysis, we show that the hybrid ALOHA protocol out-performs other ALOHA protocols in terms of all performance metrics, for both cooperative and non-cooperative scenarios. Additionally, we provide insights into the retransmission probabilities that optimize the performance metrics of interest for both scenarios.
ENTPD1 (CD39) and NT5E (CD73) expression in human Medulloblastoma: an in silico analysis
Authors: Stefani Marco Antônio , Braganhol Elizandra , Santos Guilherme Tomasi , Suwa Samuel Masao , Cabeleira Daiane Dias , Andrade Guilherme Pamplona Bueno 
Published: November 2023

Abstract
Medulloblastoma is the most common malignant tumor in the pediatric population. Its classification has incorporated key molecular variations alongside histological characterization. CD39 (also known as ENTPD1) and CD73 (also known as NT5E), enzymes of the purinergic signaling pathway, act in synergy to generate extracellular adenosine, creating an immunosuppressive tumor microenvironment. Our study examined the expression of mRNA of these genes in previously described transcriptome data sets of medulloblastoma patient samples from the Cavalli Cohort (n =763). Survival distribution was estimated according to the Kaplan–Meier method using a median cut-off and log-rank statistics; p ≤0.05. The high expression of NT5E and ENTPD1 in non-WNT and non-SHH medulloblastoma Group 4 was significantly related to a lower survival (p =2.7e-04;p =2.6e-03). The high expression of NT5E in the SHH-activated group (n =172) was significantly related to greater overall survival (p =0.017), while high expression of ENTPD1 was significantly related to lower overall survival (p =7.8e-03). The expressions of NT5E and ENTPD1 were not significantly correlated with overall survival in the WNT group (n =63;p =0.212;p =0.101). The expression of NT5E in non-WNT and non-SHH medulloblastoma Group 3 (n =113) was not significantly related to survival of patients (p= 0.124), while high expression of ENTPD1 was significantly related to greater survival (p =0.034). This in silico analysis indicates that ENTPD1 (CD39) and NT5E (CD73) can be seen as potential prognostic markers and therapeutic targets for primary medulloblastomas in non-WNT and non-SHH Group 4.
Boosting biomass, lipid content, carbon sequestration capacity of microalgae cultivated with aggregation-induced emission light-converting films
Authors: Song Yingjie , Xie Lixuan , Zhang Xiuwen , Hu Zhangli , Li Shuangfei , Zhang Pengfei , Yang Xuewei 
Published: November 2023

Abstract
Microalgae are a kind of promising raw material for bioenergy production and carbon sequestration. To enhance cell growth and production efficiency, great efforts were made for exploring the appropriate intensity and quality of light to improve the synthesis of the value-added products. The aggregation-induced emission (AIE) material (Astrazon Brilliant Red 4G), active for converting blue-green light to red light (630–680 nm), was for the first time applied in the research to achieve high fatty acid yield with considerable carbon-neutral ability. After 10 days-incubation, it revealed that AIE light-converting films boosted the biomass of microalgae Scenedesmus obliquus, Phaeodactylum tricornutum, and Chlamydomonas reinhardtii by 13.00%, 28.00%, and 26.00%, and the total lipid contents increased by 34.60%, 17.74%, and 22.63%, with the carbon sequestration capacity raised by 17.18%, 38.89%, and 35.71%. Furthermore, AIE light-converting films promoted the accumulation of unsaturated fatty acids (UFAs) in all three microalgae. Our study demonstrated the potential application of AIE light-converting films for cultivating microalgae to capture high carbon dioxide for photosynthesize carbon-neutral energy and high-valued active compounds.
Factors associated with early inhospital adverse outcome following surgery for acute appendicitis in Uganda: A multicenter cohort
Authors: Sharif Farhan Yusuf , Abraha Demoz , Edyedu Isaac , Molen Selamo Fabrice , Mauricio William , Odong Samuel Oledo , Mugeni Michael , Muhumuza Joshua 
Published: November 2023

Abstract

Introduction:
The surgery for acute appendicitis has been associated with significant morbidity. This study aimed to determine the factors associated with early inhospital adverse outcome following surgery for acute appendicitis in Uganda.
Methods
This was a multicentre prospective cohort in which early inhospital outcome following surgery for acute appendicitis was assessed at 4 regional referral hospitals in Uganda. The occurrence of complications during the admission period was documented as well as the length of hospital stay. The factors associated with the adverse outcomes were determined using Poisson regression in SPSS version 26.
Results
Of the 102 patients that underwent surgery for acute appendicitis, majority were males 79(77.5%) with a mean age of 23.8(SD = 12.5) years. Perforated appendix was seen in 26 (25.5%) patients. Post-operative complications occurred in 21(20.6%) with the commonest being surgical site infection seen in 19(18.6%) patients. The median length of hospital stay was 3(IQR = 3–4) days with 43(42.2%) staying in hospital for more than 3 days. Presence of anemia (Hb < 8) (aRR = 1.376, CI = 1.159–1.634, P = < 0.001) and having a perforated appendix (aRR = 1.263, CI = 1.026–1.555, P = 0.027) were independently associated with occurrence of complications while being HIV positive (aRR = 1.379, CI = 1.105–1.721, P = 0.005) and having a perforated appendix (aRR = 1.258, CI = 1.019–1.554, P = 0.033) were independently associated with prolonged hospital stay.
Conclusion
Sensitization of the community about early presentation is still required in order to reduce the number of patients that present late, which will in turn reduce the risk of complications and length of hospital stay.
Higher order asymptotic crack-tip fields in simplified strain gradient elasticity
Authors: Solyaev Yury 
Published: November 2023

Abstract

Explicit representation for the higher order in-plane crack tip fields is derived by using Papkovich-Neuber stress functions within the simplified strain gradient elasticity (SGE). Presented solution has separable form and contains classical Williams' series as the particular case, when the gradient effects are negligible. The leading terms in the derived solution coincide with the previously known asymptotic solutions for the crack problems in SGE. The higher order terms have coupled amplitude factors and modified definitions for the angular distribution in comparison to the classical solution. Derived asymptotic fields are compared to the full-field numerical solution for the Mode I crack problem to quantify the amplitude factors and the zones of dominance for up to eight terms.
Environmentally acquired gut-associated bacteria are not critical for growth and survival in a solitary bee, Megachile rotundata
Authors: Brar Gagandeep , Floden Madison , McFrederick Quinn , Rajamohan Arun , Yocum George , Bowsher Julia 
Published: November 2023

Abstract

Background
Social bees have been studied extensively for their gut microbial symbiosis and its beneficial functions to the host’s health. But the significance of the environmentally acquired, non-symbiotic gut microbiota in solitary bees remain inconclusive. Solitary bee, Megachile rotundata females provision their offspring with pollen from ​​​various ​plant species harboring diverse microbes that colonizes larval gut. The Apilactobacillus is the most abundant microbe, but evidence regarding the effects of Apilactobacillus and ​other provision microbes on growth and survival ​are ​lacking. We ​hypothesized that the presence of intact microbial communities especially, Apilactobacillus would enhance larval and prepupal development, weight, and survival. Conversely, the absence of intact microbial communities was expected to have a negative impact on bee fitness. We reared larvae on pollen provisions with natural microbial communities (Natural pollen) or without microbes (Sterile pollen). We also assessed the impact of Apilactobacillus micheneri by adding it to both types of pollen provisions.
Results
Feeding larvae with sterile pollen + A. micheneri led to the highest mortality. Larval development was significantly delayed in groups fed with sterile pollen and sterile pollen + A. micheneri compared to the control. Interestingly, larval and prepupal weights did not significantly differ when they were given sterile pollen, sterile pollen along with A. micheneri, and natural pollen along with A. micheneri, in comparison to those fed only natural pollen. 16S rRNA gene sequencing found a dominance of endosymbiont Sodalis when A. micheneri was introduced to natural pollen.
Conclusion
This study highlights that reliance on non-host specific, environmental bacteria do not shape the fitness of M. rotundata and may hold true for other solitary bees. Apilactobacillus micheneri is not necessarily a beneficial microbe, and is harmful if present in abundance without other microbes. The presence of Sodalis with abundant A. micheneri suggests potential crosstalk between both, shaping bee nutrition and health. This perspective will enable us to gain a deeper understanding of the significance of environmentally acquired microbiomes for the survival, growth, and development of other solitary bees.
Ki-67, Syn, TTF-1 and nomogram for predicting overall survival in primary small cell carcinoma of the esophagus
Authors: Lyu Si-Mian , Tan Li-Yan 
Published: November 2023

Abstract
Background Esophageal cancer is the eighth most diagnosed cancer and the sixth most common cause of cancer death in the world. Primary small cell carcinoma of the esophagus (PSCCE) is a rare and highly malignant neuroendocrine tumor. Identifying reliable biomarkers is essential for predicting prognosis and inferring survival for patients.Methods Information on 68 patients diagnosed with PSCCE were collected. Cox proportional hazards regression is used to determine independent prognostic factors. A nomogram was constructed to predict survival and validated.Results A total of 68 PSCCE patients were enrolled in the construction cohort. Ki-67, Syn, TTF-1, AJCC-T stage, AJCC-N stage were independent prognostic factors. The nomogram was constructed, and the calibration curve showed satisfactory agreement. The C-index was 0.726(95% CI 0.720–0.732).Conclusion The correlation between Ki-67, Syn, and TTF-1 and patient diagnosis, prognosis, and survival was found, as well as a series of factors associated with the survival of patients with PSCCE. Based on the identified factors, a nomogram predicting the survival of patients with PSCCE was generated. The predictive model demonstrated satisfactory discrimination and calibration, which can inform survival estimation and individualized treatment decisions.
Assessing the difference between radiographic and non-radiographic axial spondylarthritis in Mexican patients: The influence of clinical, demographic, laboratory and clinimetric features
Authors: John Londono , Cesar Pacheco-Tena , Maria Santos Ana , Humberto Cardiel Mario , Rodríguez-Salas Gustavo , Igor Rueda , Sofía Arias-Correal , Mesa Cristian , Juliana Mantilla Marta , Camilo Santacruz Juan , Camilo Rueda Juan , Gilberto Vargas-Alarcón , Rubén Burgos-Vargas 
Published: November 2023

Abstract

Introduction/Objectives
To compare the demographic, clinical, and laboratory characteristics, disease onset, and clinical features of radiographic axial Spondyloarthritis (r-AxSpA) and non-radiographic axial Spondyloarthritis (nr-AxSpA) patients.
Method
All patients who attended outpatient spondylarthritis clinics at Hospital General de Mexico and the Instituto Nacional de la Nutrición, from 1998 to 2005, and met the spondyloarthropathies study group criteria (EESG) were included. In addition, patients were reclassified according to The Assessment of SpondyloArthritis international Society (ASAS) criteria. We selected patients with nr-AxSpA and r-AxSpA and compared clinical, demographic, and clinimetric variables.
Results
148 Mexican patients were included. 55 (37.2%) patients had r-AxSpA, and 70 (47.3%) had nr-AxSpA. Nr-AxSpA patients had a lower proportion of males (58.6% vs 78.2%, P < 0.05), lower HLA-B27 frequency (54.3%. vs. 92.7%, P < 0.05), older at disease onset (21 vs 16 years; P < 0.01), a higher frequency of infections at disease onset (32.9 .vs 9.1%, P < 0.05) than r-AxSpA. Logistic regression showed that the factors that most influenced the presentation of r-AxSpA were history of uveitis (OR 14, 95% CI 2.3 – 85), HLA-B27 (OR 7.97, 95% CI, 2.96 – 122), gender (male) (OR 6.16, 95% CI, 1.47 – 25.7), axial enthesopathy count (OR 1.17 95% CI, 1.03 – 1.33).
Conclusions
This study provides insight into the differences between nr-AxSpA and r- axSpA. Patients with r-AxSpA were mainly male, with a younger presentation age, a higher prevalence of HLA-B27, more history of uveitis, fewer episodes of dactylitis, more axial enthesopathy, and higher disease activity.
A Mendelian Randomisation Study to Examine the Causal Associations of Circulating Micronutrient Levels with Frailty Risk
Authors: Kuribanjiang Kaidiriyan , Min Yiping , Yan Shikang , Chen Simin , Aiheti Nuerbiyamu , Wupuer Abudunaibi , Wang Jie , Peng Xing , Li Yihan , Li Huilong , Dong Yiran , Fan Yuanlin , Yang Lei , Zhao Jianping 
Published: November 2023

Abstract
Background
There is some disagreement regarding the association between circulating micronutrient levels and the risk of frailty, according to prior observational research. Using a two-sample Mendelian Randomisation (TSMR) method, we explored the causal relationship between circulating micronutrient levels and the risk of frailty.
Methods
We gathered and screened instrumental variables (IVs) for eight circulating micronutrients, including selenium, zinc, vitamin B12, vitamin B6, folic acid, and vitamins C, D, and E, from published genome-wide association studies (GWAS) and the IEU OpenGWAS open database. Frailty summary data were generated from meta-analyses of genome-wide association studies (GWAS) found in the UK Biobank (n = 164,610) and Twin Gene (n = 10,616). We ran three studies: a main analysis using independently released GWAS data, a secondary analysis using IVs filtered from OpenGWAS data, and a meta-analysis of the findings from the two MR analyses.
Results
For circulating levels of the eight micronutrients, the pooled βs were − 0.02 (95% CI: -0.090, 0.001; vitamin C), -0.021 (95% CI: -0.045, 0.001; vitamin B12), 0.029 (95% CI: -0.013, 0.072; folic acid),-0.059 (95% CI: -0.183, -0.065; vitamin D), -0.011 (95% CI: -0.099, 0.075; vitamin E), 0.006 (95% CI: -0.047, 0.061; vitamin B6), 0.004 (95% CI: -0.007, 0.017; zinc) and − 0.005 (95% CI:-0.018, 0.008; selenium).
Conclusions
To summarize, there is no evidence for a connection between the occurrence of frailty and the eight micronutrients, suggesting that supplementation with these nutrients may have little therapeutic value in preventing frailty.
Chemical control of wheat sunn pest, Eurygaster integriceps Put. by the UAV sprayer
Authors: SHEIKHIGARJAN AZIZ , Safari Mahmoud , Ghazi Mostafa Mansour , Zarnegar Ali , Shahrokhi Shahram , Moein Saeed , Seyedin Peyman 
Published: November 2023

Abstract
The sunn pest is one of the most important pests of wheat crops in Iran and the Middle East. Chemical control by spraying is the most common method of controlling this pest. In general, ground sprayers that are currently used are not equipped with advanced spraying technologies and have low efficiency. This study aimed to investigate the efficacy of using an Unmanned Aerial Vehicles (UAV) sprayer for the chemical control of wheat sunn pest. The effectiveness, field capacity, and costs of chemical insecticide application were compared by the drone sprayer and the knapsack Micronair sprayer. We found that the chemical control effectiveness of the two spraying methods was not significantly different. Both drone sprayer and knapsack Micronair sprayer had an effectiveness percentage of more than 95%. The drone sprayer was better than the knapsack Micronair in terms of field capacity, insecticide dosage ml ha− 1, spray volume L ha− 1, reduced drift, and the probability of user contamination. The field capacity of the drone sprayer and the knapsack Micronair sprayer was 5.5 and 0.8 hectares per hour, respectively, and the spray volume was 14.6 and 30.0 L ha− 1, respectively.