bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incentivized fake reviews: When cognitive reappraisal paves the way for an immoral journey
Authors: Malik Aaminah Zaman , Sadeghi R Kiarash , Paswan Audhesh , Kanwal Fizza 
Published: February 2024

AbstractRecent studies explain how to detect fake reviews and their impacts on consumers and businesses. Despite the prevalence and significance of fake reviews, prior research has yet to fully explore the motivations and psychological mechanisms behind voluntary participation in writing fake reviews, especially in incentivized campaigns. This paper explains why consumers engage in the immoral act of writing fake reviews in return for free products. We investigate the interactive role of individuals' self‐focused motivations and cognitive reappraisal ability in diminishing consumers' moral emotions of guilt, subsequently influencing their intention to write incentivized reviews. Built on three studies, the findings reveal that customers' self‐focused motivations coupled with their ability to regulate emotions dissuade their feelings of guilt. Consequently, customers who experience downregulated emotions of guilt show a higher intention to write incentivized fake reviews. This study contributes to the existing literature by illustrating the driving factors toward writing incentivized fake reviews, especially by depicting the negative role of cognitive reappraisal. The study also has managerial implications indicating that such customer behavior can be avoided by fostering a sense of consideration toward others by making them aware about the impact of their actions.
An Investigation into the Larvicidal Activity of Biologically Synthesized Silver and Copper Oxide Nanoparticles Against Mosquito Larvae
Authors: Narayanan Lakshmanan , S R Suseem , Kamaraj Chinnaperumal 
Published: February 2024

AbstractThis study is primarily focused on the synthesis of silver and copper oxide nanoparticles utilizing the extract of Ipomoea staphylina plant and their larvicidal activity against specific larvae. Notably, Anopheles stephensi and Aedes aegypti are significant disease vectors responsible for transmitting diseases such as malaria, dengue fever, Zika virus, and chikungunya (Anopheles stephensi), and dengue and Zika (Aedes aegypti). These mosquitoes have a substantial impact on urban areas, influencing disease transmission dynamics. In an effort to control these larvae, we have pursued the synthesis of a herbal‐based nanomedicine derived from I. staphylina, a valuable herb in traditional medicine. Our successful synthesis of silver and CuO nanoparticles followed environmentally sustainable green chemistry methodologies. The I. staphylina plant extract played a dual role as a reducing agent and dopant, aligning with principles of sustainability. We employed X‐ray diffraction (XRD) analysis to validate the nanoparticle structure and size, while field‐emission scanning electron microscopy (FE‐SEM) revealed well‐defined nanostructures. Elemental composition was determined through energy‐dispersive X‐ray (EDX) analysis, and UV‐visible spectroscopy provided insights into the bandgap energy (3.15 eV for silver, 1.2 eV for CuO nanoparticles). These nanoparticles exhibited robust larvicidal activity, with CuO nanoparticles surpassing silver nanoparticles in terms of LC50 and LC90 values. Moreover, the developmental toxicity of CuO and Ag NPs was evaluated in zebrafish embryos as part of non‐target eco‐toxicological studies conducted in a standard laboratory environment. These findings underscore the potential utility of these nanoparticles as highly effective and environmentally friendly natural pesticides, offering cost‐effectiveness and ecological benefits.
In silico analysis of chromium‐reducing OXR genes derived from tannery effluent‐contaminated soil metagenome
Authors: Singh Ayushi , Patel Sanjay K. S. , Varma Ajit , Mandal Manabendra , Porwal Shalini 
Published: February 2024

AbstractTannery effluent waste comprises various potentially toxic metals, including chromium (Cr) with varying acute or chronic toxicity. Cr(VI) is known to be a category‐A carcinogen. Reduction of toxic Cr(VI) to Cr(III), which has lesser bioavailability, is one of the mechanisms used by many microbes to withstand Cr(VI) toxicity in the contaminated effluents. Oxidoreductase (OXRs) reduces toxic Cr(VI) to Cr(III); hence a thorough understanding of the OXRs is important for developing a suitable strategy to minimize Cr(VI) toxicity. Therefore, the OXR‐encoding genes were sequenced using metagenomic DNA shotgun sequencing from the tannery effluent‐contaminated soil. Six OXR‐encoding genes were expressed in Escherichia coli, and OXR activity was confirmed by in situ quantitative assays. The six proteins were subjected to phylogenetic and evolutionary analysis. Further, detailed structural analysis of the two OXRs, namely, OXR3 and OXR8 with lowest and highest activity respectively, were investigated in silico for structural characteristics. The results revealed that both the proteins were soluble FMN‐linked oxidoreductases. Eight conserved active site residues (Pro24, Thr26, Ala59, Tyr139, His178, Tyr180, His219, Tyr221, Arg269, and Lys360) in the enzyme OXR3 were predicted. Similarly, nine conserved active site residues (Pro20, Thr22, Ala55, Glu97, His191, Tyr193, Arg241, Cys334, and Arg335) were predicted in OXR8. The tertiary structure of OXR8 was an aldolase TIM barrel structure, like Thermus scotoductus chromate reductase. Docking with FMN revealed the involvement of all the nine predicted active site residues in FMN binding with Pro20, Thr22, and Cys334 as the most important ones.
SWOG S1820: A pilot randomized trial of the Altering Intake, Managing Bowel Symptoms Intervention in Survivors of Rectal Cancer
Authors: Sun Virginia , Guthrie Katherine A. , Crane Tracy E. , Arnold Kathryn B. , Colby Sarah , Freylersythe Sarah G. , Braun‐Inglis Christa , Topacio Roxanne , Messick Craig A. , Carmichael Joseph C. , Muskovitz Andrew A. , Nashawaty Mohammed , Bajaj Madhuri , Cohen Stacey A. , Flaherty Devin C. , O’Rourke Mark A. , Jones Lee , Krouse Robert S. , Thomson Cynthia A. 
Published: February 2024

AbstractBackgroundSurvivors of rectal cancer experience persistent bowel dysfunction after treatments. Dietary interventions may be an effective approach for symptom management and posttreatment diet quality. SWOG S1820 was a pilot randomized trial of the Altering Intake, Managing Symptoms in Rectal Cancer (AIMS‐RC) intervention for bowel dysfunction in survivors of rectal cancer.MethodsNinety‐three posttreatment survivors were randomized to the AIMS‐RC group (N = 47) or the Healthy Living Education attention control group (N = 46) after informed consent and completion of a prerandomization run‐in. Outcome measures were completed at baseline and at 18 and 26 weeks postrandomization. The primary end point was total bowel function score, and exploratory end points included low anterior resection syndrome (LARS) score, quality of life, dietary quality, motivation, self‐efficacy, and positive/negative affect.ResultsMost participants were White and college educated, with a mean age of 55.2 years and median time since surgery of 13.1 months. There were no statistically significant differences in total bowel function score by group, with the AIMS‐RC group demonstrating statistically significant improvements in the exploratory end points of LARS (p = .01) and the frequency subscale of the bowel function index (p = .03). The AIMS‐RC group reported significantly higher acceptability of the study.ConclusionsSWOG S1820 did not provide evidence of benefit from the AIMS‐RC intervention relative to the attention control. Select secondary end points did demonstrate improvements. The study was highly feasible and acceptable for participants in the National Cancer Institute Community Oncology Research Program. Findings provide strong support for further refinement and effectiveness testing of the AIMS‐RC intervention.
Tumour‐associated macrophage‐derived DOCK7‐enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis
Authors: Chen Weiwei , Zhou Menghua , Guan Bingjie , Xie Bowen , Liu Youdong , He Jiang , Zhao Jingjing , Zhao Qian , Yan Dongwang 
Published: February 2024

AbstractBackgroundMetastasis accounts for the majority of deaths among patients with colorectal cancer (CRC). Here, the regulatory role of tumour‐associated macrophages (TAMs) in CRC metastasis was explored.MethodsImmunohistochemical (IHC) analysis of the TAM biomarker CD163 was conducted to evaluate TAM infiltration in CRC. Transwell assays and an ectopic liver metastasis model were established to evaluate the metastatic ability of tumour cells. RNA sequencing (RNA‐seq) and liquid chromatography‒mass spectrometry (LC‒MS) were applied to identify the differentially expressed genes and proteins in CRC cells and in TAM‐derived extracellular vesicles (EVs). Cholesterol content measurement, a membrane fluidity assay and filipin staining were performed to evaluate cholesterol efflux in CRC cells.ResultsOur results showed that TAM infiltration is positively correlated with CRC metastasis. TAMs can facilitate the migration and invasion of MC‐38 and CT‐26 cells via EVs. According to the RNA‐seq data, TAM‐EVs increase cholesterol efflux and enhance membrane fluidity in CRC cells by regulating ABCA1 expression, thus affecting the motility of CRC cells. Mechanistically, DOCK7 packaged in TAM‐EVs can activate RAC1 in CRC cells and subsequently upregulate ABCA1 expression by phosphorylating AKT and FOXO1. Moreover, IHC analysis of ABCA1 in patients with liver‐metastatic CRC indicated that ABCA1 expression is significantly greater in metastatic liver nodules than in primary CRC tumours.ConclusionsOverall, our findings suggest that DOCK7 delivered via TAM‐EVs could regulate cholesterol metabolism in CRC cells and CRC cell metastasis through the RAC1/AKT/FOXO1/ABCA1 axis. DOCK7 could thus be a new therapeutic target for controlling CRC metastasis.
Geospatial environmental complexity, spatial brain volume, and spatial behavior across the Alzheimer's disease spectrum
Authors: Shin Naewoo , Rodrigue Karen M. , Yuan May , Kennedy Kristen M. 
Published: February 2024

AbstractINTRODUCTIONUnderstanding impact of environmental properties on Alzheimer's disease (AD) is paramount. Spatial complexity of one's routinely navigated environment is an important but understudied factor.METHODSA total of 660 older adults from National Alzheimer's Coordinating Center (NACC) dataset were geolocated and environmental complexity index derived from geospatial network landmarks and points‐of‐interest. Latent models tested mediation of spatial navigation‐relevant brain volumes and diagnosis (cognitively‐healthy, mild cognitive impairment [MCI], AD) on effect of environmental complexity on spatial behavior.RESULTSGreater environmental complexity was selectively associated with larger allocentric (but not egocentric) navigation‐related brain volumes, lesser diagnosis of MCI and AD, and better spatial behavioral performance, through indirect hierarchical mediation.DISCUSSIONFindings support hypothesis that spatially complex environments positively impact navigation neural circuitry and spatial behavior function. Given the vulnerability of these very circuits to AD pathology, residing in spatially complex environments may be one factor to help stave off the brain atrophy that accompanies spatial navigation deficits across the AD spectrum.
A healthier movement behavior profile is associated with body‐congruent food choices through self‐determined motivations to exercise and regulate eating
Authors: Carraça Eliana Veiga , Rodrigues Bruno , Sánchez‐Oliva David 
Published: February 2024

AbstractPhysical activity (PA) facilitates eating behavior regulation, in part through self‐determined motivations to regulate PA and eating behaviors. However, different patterns of PA and sedentary behavior (SB) accumulation appear to have distinct effects on several health outcomes and might therefore be differently related to eating self‐regulation. This study aimed to identify different PA/SB profiles, and test motivational routes underlying associations between these profiles and eating. A total of 297 adults (age: 34.5 ± 10.6 years; BMI: 23.2 ± 3.4 kg/m2) filled a psychometric battery of validated questionnaires to assess PA, SB, motivations to exercise and eating, and intuitive eating dimensions. Latent Profile Analysis and Preacher and Hayes Mediation Procedures were conducted. A 3‐profiles model was retained as the best solution (++sitting/‐PA, n = 89; ++PA/‐sitting, n = 44; +PA/+sitting, n = 164). Participants in the ++PA/‐Sitting profile showed higher self‐determined motivations to exercise (ps < 0.001) and regulate eating (ps < 0.05), mostly when compared to the ++Sitting/‐PA profile. Mediation analyses showed that participants in the ++PA/‐Sitting profile (vs. ++Sitting/‐PA) made more body‐congruent food choices, in part through a serial mediation effect of self‐determined motivations to exercise and eating (Estimate: 0.25 (0.07), 95% CI: 0.13; 0.40). All analyses were adjusted for age, gender, and BMI. Results extend prior evidence, showing that motivations underpinning PA/eating have better quality when greater levels of PA are combined with lower sitting time, and by showing that self‐determined motivations partly mediate the effects of different PA/SB profiles on eating regulation. These findings have implications for future interventions promoting active lifestyles.
An examination of differences in epigenetic methylation of saliva type samples based on collection method
Authors: Ghemrawi Mirna , Fernandez‐Tejero Nicole , Vaquero Lia , Wanna Amani , Carmel Justin H. , McCord Bruce 
Published: February 2024

AbstractIn the context of forensic casework, it is imperative to both establish a DNA profile from biological specimens and accurately identify the specific bodily fluid source. To achieve this, DNA methylation markers have been developed for the differentiation of blood, semen, vaginal epithelial secretions, and saliva samples. Saliva, alternatively referred to as oral fluid, is recognized for its heterogeneous cellular composition, characterized by a mixture of epithelial, leukocytic, and bacterial cells. Consequently, our research has revealed variations in methylation percentages that correlate with the method employed for collecting saliva samples. To investigate these concepts, we scrutinized four CpG markers situated within or in proximity to the BCAS4, SLC12A8, SOX2OT, and FAM43A genes. Subsequently, we designed primers based on bioinformatically transformed reference sequences for these markers and rigorously assessed their quality by examining dimer and hairpin formation, melting temperature, and specificity. These loci were identified as saliva markers based on either buccal swabs or spit collection. Yet, there has been minimal or no research conducted to explore the variations in methylation between different collection methods. For this study, buccal, lip, tongue, spit, and nasal swabs were collected from 20 individuals (N = 100). Mock forensic samples, which include chewing gum (N = 10) and cigarettes (N = 10), were also tested. DNA was extracted, bisulfite converted, then amplified using in‐house designed assays, and pyrosequenced. The methylation levels were compared to other body fluids (semen, blood, vaginal epithelia, and menstrual blood [N = 32]). A total of 608 pyrosequencing results demonstrated that sampling location and collection method can greatly influence the level of methylation, highlighting the importance of examining multiple collection/deposition methods for body fluids when developing epigenetic markers.
Clinical outcomes of cetuximab‐based treatment for distant metastatic head and neck squamous cell carcinoma: A real‐world study using Taiwan Head Neck Society registry database
Authors: Lu Hsueh‐Ju , Hsieh Meng‐Che , Wang Hung‐Ming , Hsieh Jason Chia‐Hsun , Yen Chia‐Jui , Wu Shang‐Yin , Huang Huai‐Cheng , Wang Hui‐Ching , Chu Pen‐Yuan , Chen Tien‐Hua , Chien Chih‐Yen , Huang Tai‐Lin , Chang Yi‐Fang , Hua Chun‐Hung , Lien Ming‐Yu , Chen Jo‐Pai , Lu Wei‐Chen , Lin Jin‐Ching , Wang Chen‐Chi , Liu Yi‐Chun , Yang Muh‐Hwa , Lou Pei‐Jen 
Published: February 2024

AbstractBackgroundFor R/M HNSCC, the differences in prognosis and treatment options between distant metastasis (DM) and locoregional recurrence, especially in the DM group, remain unclear.MethodsFrom the Taiwan Head Neck Society registry database, patients who were diagnosed with R/M HNSCC and received cetuximab‐based frontline therapy were collected for analysis.ResultsAmong the enrolled patients, 59.3% (491/827) belonged to the DM group. The DM group had less primary site of oral cavity, less betel nut chewing, higher lactate dehydrogenase (LDH) levels, and higher LDH/albumin ratio compared with the non‐DM group. For the patients with primary site of oral cavity and current smokers, DM coexisted with poorer outcomes. In the DM group, EXTREME‐like regimen was more suitable for older patients, those with elevated LDH, and those with higher LDH/albumin ratio than TPExtreme‐like regimen.ConclusionDM coexisted with poorer prognosis in certain groups. LDH‐associated biomarkers may aid treatment options for DM patients.
Portal Hypertensive Gastropathy in Children with Extrahepatic Portal Venous Obstruction: Role of Variceal Obliteration by Endoscopic Sclerotherapy and Helicobacter pylori Infection
Authors: Yachha S. K. , Ghoshal U. C. , Gupta R. , Sharma B. C. , Ayyagari A. 
Published: February 2024

SummaryWe aimed to determine the frequency of portal hypertensive gastropathy (PHG) in children with extrahepatic portal venous obstruction (EHPVO) and to find out the role of esophageal variceal obliteration by endoscopic injection sclerotherapy (EIS) and Helicobacter pylori (H. pylori) infection in the pathogenesis of PHG. Twenty consecutive children were studied before EIS and 20 after esophageal varices obliteration. Diagnosis of PHG was established by endoscopic assessment. Helicobacter pylori infection was diagnosed by rapid urease test, H. pylori culture, smear, and histopathologic examination of antral biopsy specimens. Portal hypertensive gastropathy was characterized and graded by endoscopy according to previously established criteria. In the pre‐EIS group, eight (40%) had PHG (mild in all) compared with 16 (80%; mild in eight, severe in eight) in the post‐EIS group (p < 0.05). Portal hypertensive gastropathy was more extensive in the post‐EIS group. There was no significant difference between frequency of gastric varices and H. pylori infection in the pre‐EIS and post‐EIS groups. We conclude that PHG is common in children with EHPVO; its frequency, extent, and severity increase after esophageal variceal obliteration by EIS, and H. pylori infection does not play any role in its development.
Genotyping of Hepatitis C Virus: Coinfection by Multiple Genotypes Detected in Children with Chronic Posttransfusion Hepatitis C
Authors: Matsubara Takako , Sumazaki Ryo , Shin Kenji , Nagai Yoji , Takita Hitoshi 
Published: February 2024

SummaryThe distribution of genotypes of the hepatitis C virus (HCV) was studied in 24 children with chronic posttransfusion hepatitis C. Genotypes were determined by reverse transcription‐nested polymerase chain reaction with type‐specific primers. Twenty (83%) were infected by a single genotype: 14 by type II [1b], 3 by III [2a], and 3 IV [2b]. Four (17%) were coinfected by two genotypes. The amount of blood transfusion given to the patients infected by multiple genotypes was significantly larger than to those infected by a single genotype (mean ± SD, 15.2 ± 14.4 vs 78.6 ± 42.0 U). Three of the four patients infected by multiple genotypes were considered to be immunocompromised by anticancer therapy for malignant disease. Eighteen patients showed a raised level of alanine aminotransferase throughout the follow‐up, while the remaining six patients (three of type II [1b], two of IV [2b], and one of III [2a] + IV [2b]) achieved biochemical remission. Liver biopsy was performed on 19 patients. Compared to those with type III [2a] or IV [2b], those with type II [1b] appeared to show more severe histological changes with higher histological activity index scores, although there was no significant difference. The positive rates of antibody to C100‐3 or 5‐1‐1 in patients with type III [2a] or IV [2b] were lower than in those with type II [1b] (33 and 33 vs 43 and 50%), whereas the antibody to C33C or C22‐3 was detected in nearly all patients regardless of their genotypes. In the present study, we found a high incidence of multiple‐genotype infection among children with chronic posttransfusion hepatitis C.
IgA Endomysium Antibodies on Human Umbilical Cord: An Excellent Diagnostic Tool for Celiac Disease in Childhood
Authors: Kolho Kaija‐Leena , Savilahti Erkki 
Published: February 2024

Background:An improvement in screening for celiac disease has recently been described that uses human umbilical cord as a substitute for monkey esophagus to determine IgA endomysium antibodies in adults. As using monkey esophagus is ethically questionable for large‐scale screening, we studied whether substitution of umbilical cord would be suitable for pediatric patients as well.Methods:Serum from 53 children with untreated celiac disease, 22 in remission and 13 on challenge, were screened for antigliadin IgA, antigliadin IgG, and IgA reticulin antibodies, in addition to IgA endomysium antibodies tested both on monkey esophagus and on human umbilical cord. Controls included 20 patients with cow‐milk‐sensitive enteropathy, 23 with inflammatory bowel disease, and 23 with diabetes mellitus, and 48 patients who were biopsied to exclude celiac disease either because of positive gliadin antibody test or disturbed growth.Results:Sensitivity (0.94) and specificity (1.0) were similar for umbilical cord and esophageal determinations in active celiac disease. Both substrates detected identical positive cases and neither gave false‐positive results. In celiac patients on a gluten‐free diet, endomysium antibodies with either substrate were positive in seven identical cases and negative in 15 of 22 cases. Correlations with reticulin antibodies were comparable with human umbilical cord and monkey esophagus (0.83 and 0.85, respectively; Spearman Correlation Scction Pair‐Wise deletion).Conclusions:Human umbilical cord is an excellent substitute for monkey esophagus to determine endomysium antibodies in celiac diagnosis in children and adolescents.
Enteric Cryptosporidiosis in Pediatric HIV Infection
Authors: Guarino Alfredo , Castaldo Annunziata , Russo Stefania , Spagnuolo Maria Immacolata , Canani Roberto Berni , Tarallo Luigi , DiBenedetto Linda , Rubino Armido 
Published: February 2024

BackgroundEnteric cryptosporidiosis is a frequent problem in adults with human immunodeficiency virus (HIV) infection, but little is known of its features in children. The aim of this study was to investigate the incidence and the clinical features of cryptosporidiosis in HIV‐infected children.MethodsThirty‐five children with symptomatic HIV infection were screened every 2 months, and in case of diarrhea, for the presence of Cryptosporidium. Intestinal function tests were performed, and the fecal osmotic gap was measured in children with cryptosporidiosis.ResultsSeventy episodes of diarrhea occurred in 16 children in a median period of 17 months. Cryptosporidium was detected in five cases, all with full‐blown acquired immunodeficiency syndrome. Cryptosporidiosis was significantly more protracted than any other form of diarrhea and was associated with dehydration and severe weight loss. Intestinal function was not modified during cryptosporidiosis. Osmotic gap values were consistent with secretory rather than osmotic diarrhea. In four cases, recovery was observed without specific treatment.ConclusionsEnteric cryptosporidiosis is a severe problem in advanced stages of HIV infection. It does not induce intestinal malabsorption. It induces diarrhea of secretory type. Recovery may be observed independently of therapy.