bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Long-term Progression-free Survival with Glioblastoma Patient Harboring MSH6 Pathogenic Germline Mutation: A Case Report
Authors: Zhou Yiqiang , Zhang Yanxiang , Han Tiantian , Piao Yueshan , Tang Jie 
Published: February 2025

Abstract

Background Lynch syndrome (LS) is a cancer syndrome caused by germline mutations in DNA mismatch repair genes. Patients with Lynch syndrome have higher risk of brain tumors, predominantly high-grade gliomas. LS-related gliomas have poor overall survival.
Case presentation We present a case of a31-year-old male patient with a frontal lesion by magnetic resonance imaging (MRI). The pathological diagnosis was isocitrate dehydrogenase (IDH)wildtype glioblastoma (WHO grade 4) with 30% Ki-67 proliferation index. After surgery, this patient received radiotherapy and temozolomide chemotherapy following Stupp protocol. During over 64 months follow up, no sign of tumor recurrence was found after surgery. Therefore, next-generation sequencing was suggested to this patient. The result revealed a heterozygous germline variation of c.3261dup in exon 5 of MSH6 gene which resulted in a truncated MSH6 protein (MSH6 p.F1088Lfs*5) with a tumor mutation burden of 327.36 Mut/Mb.
Conclusion MMR deficiency may lead to TMZ resistance in glioma cells. However, this case had long-term benefits from standard radio- and chemo-therapy after surgery, probably due to the distinct molecular characteristics and tumor microenvironment. This finding provided insight to perform clinical studies to investigate the molecular characterization of Lynch associated glioma patients and the role of microglia in antitumor.
Predicting trajectories of acute illness using RNA velocity of whole blood
Authors: Dunican Claire , Wilson Clare , Habgood-Coote Dominic , Patterson Suzanna , Noursadeghi Mahdad , Moseki Raymond , Stek Cari , Wilkinson Robert , Agyeman Philipp , Beudeker Coco , Biesbroek Giske , von Both Ulrich , Brengel-Pesce Karen , Carrol Enitan , Coin Lachlan , D'Souza Giselle , De Tisham , Emonts Marieke , Fidler Katy , Fink Colin , Flier Michiel Van der , Georgaki Ioanna , Kolberg Laura , Kolnik Mojca , Kuijpers Taco , Martinon-Torres Federico , Mommert-Tripon Marine , Nichols Samuel , Paulus Stéphane , Pokorn Marko , Pollard Andrew , Rivero-Calle Irene , Rudzate Aleksandra , Schlapbach Luregn , Schweintzger Nina , Shen Ching-Fen , Shrestha Shrijana , Tan Chantal , Tsolia Maria , Usuf Effua , Velden Fabian van der , Vermont Clementien , Voice Marie , Yeung Shunmay , Zavadska Dace , Zenz Werner , Wright Victoria , Levin# Michael , Herberg Jethro , Lai Rachel , Meintjes Graeme , Chiu Christopher , Barahona Mauricio , Kaforou Myrsini , Cunnington Aubrey 
Published: February 2025

Abstract

Transcriptomic analyses reveal the status of cells, tissues, or organisms, across states of health and disease. RNA velocity adds a temporal dimension to single cell analyses, predicting future transcriptomic and phenotypic states, based on current spliced and unspliced mRNA of each cell. We hypothesized that RNA velocity could be adapted to predict future clinical status of individuals with acute illness using their whole-blood transcriptome. We developed a method for quantitative prediction of transitions in clinical state from a single time-point sample, which we call VeloCD. This predicted transcriptomic trajectories and future infection status in influenza A and SARS-CoV-2 human challenge studies. In HIV-TB coinfected individuals, it predicted the onset of immune reconstitution inflammatory syndrome. In a multinational observational study of acutely unwell febrile children, VeloCD predicted those with greatest medical care requirements. Our results demonstrate a novel application of RNA velocity to predict the trajectory of acute illness.
tsRNAs sequencing reveals tRNA-Gly (GCC)-derived small RNAs as colorectal cancer biomarker
Authors: Wan Yu , Jiao Yuting , Liu Anrui , Lai Yushan , Luo Wenfeng , Zhang Xiaoying , Lin Xiaoling , Wang Xiaoyun 
Published: February 2025

Abstract

Colorectal cancer (CRC) is one of the most common gastrointestinal tumors and the second leading cause of malignancy-related death worldwide. Novel biomarkers with high sensitivity and specificity are necessary to improve the diagnosis of colorectal cancer (CRC) in terms of early diagnosis and prognosis. In this study, we obtained tsRNAs expression profiles from formalin-fixed and paraffin-embedded (FFPE) clinical tissue samples to identify novel tsRNAs with potential biomarker properties in colorectal cancer. The expression profiles of colorectal cancer tsRNAs were successfully constructed, 612 up-regulated and 439 down-regulated tsRNAs were identified in the tumor group. tRNA-Gly (GCC)-derived i-tRF-Gly-GCC and 5′-tRF-Gly-GCC were highly expressed in CRC tissues compared to the paraneoplastic tissues. The same results were found in serum from colorectal cancer patients compared to serum from healthy volunteers. Both tsRNAs were highly expressed in CRC tissues and the AUC in ROC analysis was greater than 0.7, which has clinical diagnostic value. WGCNA analysis showed that the target genes of the two tsRNAs were closely related to CRC, and the expression of the target genes was significantly decreased in the cancer groups of the COAD and READ datasets. We also performed validation experiments in HCT-116 cells, and the results confirmed that i-tRF-Gly-GCC and 5′-tRF-Gly-GCC significantly enhanced cell proliferation and migration. In conclusion, we identified and characterized two tsRNAs (i-tRF-Gly-GCC and 5′-tRF-Gly-GCC) as the biomarkers for the diagnosis of colorectal cancer.
A column-switching two-dimensional liquid chromatography - high resolution/triple quadrupole dual mass spectrometry system for simultaneously untargeted metabolome and targeted exposome analyses
Authors: Xu Guowang , Wang Yuting , Cao Penglong , Guan Pengwei , Chen Tiantian , Guo Xingyu , Shi Xianzhe , Liu Xinyu 
Published: February 2025

Abstract

Metabolome-exposome association studies (mEWAS) demands high-coverage and high-sensitivity analytical methods for metabolites and exposures with diverse chemical properties and concentrations. Here, we present an integrated column-switching two-dimensional liquid chromatography (2DLC) - high resolution/triple quadrupole dual mass spectrometry (MS) method, achieving simultaneously untargeted metabolome and targeted exposome analyses including 598 chemical exposures in a single run. Polar and non-polar compounds with log P ranging from -8 to 15 were separated without unretained compounds in the dead time. Benchmarked against biological matrices, analyte coverage increased by 35-104% over one-dimensional LC method. Highly sensitive limits of detection with 0.001-1 μg/mL for >90% metabolites and 0.001-1 ng/mL for >90% chemical exposures were obtained, matching the concentration difference between the metabolome and exposome in human blood. The method was demonstrated in patients with chronic diseases, showing the feasibility of establishing simultaneously untargeted metabolome and targeted exposome analytical methods for mEWAS by integrating 2DLC-dual MS techniques.
Financial Sustainability of Local NGOs Funded by the Churches Health Association of Zambia in Zambia. A Mixed Methods Survey.
Authors: Kachumi Michael Mutaya , Nawa Mukumbuta , Mwanakatwe Joseph , Bwembya Raymond , Menda Dhally Mutombo , Sichinga Karen Sichali , Shaikh Junaid M 
Published: February 2025

Abstract

Introduction: Local Non-Profit Organisations (LNPO) play an important role of providing services to vulnerable communities especially in rural areas where for-profit organisations do not reach. However, sustainability of the Non-Profit Organisations is often not guaranteed as evidenced by closures of Non-Profit Organisations that were once thriving. This study assessed the financial sustainability of local organisations that are funded by the Churches Health Association of Zambia.
Methodology: The study used a mixed methods approach which comprised of a desk review, qualitative and quantitative survey. Data analysis of the qualitative component was done using the thematic analysis approach. Quantitative data was analysed using the Statistical Package for the Social Sciences (SPSS) version 22; descriptive and inferential statistics was done by summarization of the findings which included an assessment of the financial position and operational activity of the NPOs and multivariable linear regression.
Results: A total of 87 participants were interviewed in the survey and 21 participated in the qualitative in-depth interviews. CHAZ's significantly contributed to the financial suitability of the four local NPOs, with its funding heavily relied upon by NPO 2 (up to 99%) and less so by others (8-19%). Operating margins fluctuated widely, with three NPOs averaging negative margins, reflecting financial instability. Cash reserves varied, showing limited liquidity across NPOs. Regression analysis identified Financial Planning and Strategy and Leadership as key contributors to financial sustainability, while Sound Administration negatively impacted it. From interviews, challenges included limited donor funding, restricted domestic resource mobilization, economic instability, and internal issues such as inadequate long-term planning and founder dependency.
Conclusion: The study highlighted that funding from CHAZ significantly enhances the financial sustainability of LNPOs in Zambia by promoting income diversification and reducing reliance on single funding sources. However, it encourages that there should be a need for the studied LNPOs to expand funding avenues beyond CHAZ to help mitigate risks and longer financial viability. Organizations with positive reserve ratios are financially stable, but those with low or negative reserves face vulnerabilities. Key factors like strategy, leadership and financial planning related to improved financial sustainability.
Shared structural mechanisms of alternating access between the secondary peptide transporter SbmA and ABC transporters
Authors: Beis Konstantinos , Ettema Thijs , Inaba-Inoue Satomi , Thangaratnarajah Chancievan , Silva Leticia Alves da , Clarke Amy , Stepien Piotr , Shah Anokhi , Ma Yue , Hardman Katie , David Sophia , El-Mkami Hassane , Heddle Jonathan , Nomura Norimichi , Ogasawara Satoshi , Iwata So , Ghilarov Dmitry , Pliotas Christos , Stockner Thomas , Slotboom Dirk 
Published: February 2025

Abstract

SbmA is a membrane transporter from Escherichia coli that imports antimicrobial peptides. Although the protein is a secondary transporter that is energized by the proton gradient, it is structurally related to the transmembrane domain (TMD) of ATP-binding cassette (ABC) transporters. SbmA therefore bridges the structural divide between primary and secondary transporters. However, it remained unclear, if SbmA also shares the mechanism of alternating access with ABC transporters, because only a single (outward-open) state has been resolved. Here, we show by sequence analysis that SbmA has likely evolved from the TMD of an early ancestor of the ABC transporter YddA. We determined the cryogenic electron microscopy structures of SbmA in occluded and inward-facing states. These conformations closely resemble equivalent states found in ABC transporters, indicating a shared structural mechanism of transport. In contrast to ABC transporters, where nucleotide binding, hydrolysis and release steer conformational changes necessary for substrate translocation, electron paramagnetic resonance (EPR) spectroscopy and molecular dynamics (MD) simulations reveal how pH changes induce conformational transitions in SbmA, consistent with a mechanism of substrate internalization that utilizes the transmembrane proton gradient.
The Theoretical Model Construction of the Identity of Chinese Preservice Physical Education Teachers ——Based on the Grounded Theory
Authors: Zhu ShuSheng 
Published: February 2025

Abstract

Purpose
Cultivating the professional identity of preservice physical education teachers (PPETs) is crucial for their career development. However, the factors that influence their professional identity are still unclear.
Method
We reviewed the literature on the identity of PPETs and conducted interviews with 15 PPETs. The data collected was analysed using the grounded theory and Nvivo12.
Results
There are three categories of influencing factors that affect the identity of PPETs: (a) preconcept identify, which contains the anticipation for the image of teachers, impression of PE Teachers, social and cultural influence and personal characters and preferences; (b) professional learning identity, which includes curricular knowledge, counselors’ guidance and self-efficacy; (c) educational practice identity, which includes internship guidance, internship environment and internship evaluation.
Conclusion
This research result can help the education department understand the influencing factors of PPETs' identity and help PPETs obtain a good identity.
Analysis of the influence of postoperative personal care on the surgical outcomes of endoscopic dacryocystorhinostomy
Authors: Zheng Xinyu , Liu Yunming , Yu Xinyue , Wang Kerui , Meng Ziwei , Li Jing , Yang Can , Gong Chengzhen , Liang Xuanwei , Chen Rongxin , Li Shuiling 
Published: February 2025

Abstract

Purpose: To investigate the impact of early postoperative personal care of endoscopic dacryocystorhinostomy (En-DCR) on surgical outcomes.
Methods: This prospective study evaluated the early prognosis (1 month) and personal care of 90 patients with 94 eyes who underwent endoscopic dacryocystorhinostomy (En-DCR) for Chronic dacryocystitis（CD）. Surgical outcomes were divided into ostium grade and operative effect. Operative effect was judged by subjective symptom improvement, in addition to ostium assessment based on the results of nasal endoscopic review, and the relationship between operative effect and ostium grade was explored. In addition, we analyzed the key factors affecting operative effect and ostium grade during postoperative personal care.
Results: The results of correlation analysis between operative effect and objective indicators showed that the outcomes of lacrimal duct irrigation（r=0.444，P＜0.001）, DDT（r=0.217，P=0.036）, and ostium grade（r=0.350，P＜0.001） were positively correlated with the operative effect of patients. The analysis of influencing factors during postoperative nursing showed that patients with nasal saline rinsing at least once a day after operation, nasal spray (Xylometazoline Hydrochloride and Budesonide) at least once a day after operation and without postoperative respiratory tract infection had better ostium grades and operative effect (P<0.05).
Conclusions: The early surgical outcome of endoscopic dacryocystorhinostomy (En-DCR) is closely related to the condition of the ostium. During postoperative personal care, patients who maintain nasal saline rinsing and nasal spray (Xylometazoline Hydrochloride and Budesonide) at least once a day and reasonably prevent respiratory tract infection tend to have better recovery of early postoperative ostium and better operative effect. Medical staff should strengthen the home nursing guidance for patients after En-DCR day surgery.
Health Challenges in Patients with Epidermolysis bullosa: a cross-sectional study
Authors: Souza Mariana Silveira , Lopes Laura Cascão , de Almeida Luiza Grossmann , Dantas Maria Thaynná Cintra , Moura Mariela Dutra Gontijo , de Almeida Rinaldo Borges , Grossmann Soraya de Mattos Camargo 
Published: February 2025

Abstract

Purpose
Epidermolysis bullosa (EB) is a rare genetic disorder affecting connective tissue that causes painful ulcerations and oral disorders. In our study, we aimed to characterize the general and oral health of patients with EB, develop a profile of such patients, and establish prevention and treatment strategies to improve their oral health.
Methods
In a cross-sectional study conducted participants underwent anamnesis, an oral clinical evaluation, and an oral health orientation. Fisher’s test was used to assess associations between clinical variables and oral diseases observed.
Results
Twelve patients with EB were examined; 58% were female, and their mean age was 22 years. Anamnesis revealed that 67% of patients were taking medication, 92% were under medical care, 50% had prior hospitalizations, 58% had anemia, and 42% had undergone surgery. Psychological assessments showed that 58% of patients had experienced anxiety, 58% reported good self-esteem, and 31% received psychological counseling. Intraoral exams identified oral mucosal lesions in 58% of patients and carious lesions in 42%. Pain was significantly associated with mucosal lesions (p = .024), and photobiomodulation therapy using a low-level laser was performed to treat oral mucosal lesions. All patients presented better after completing the therapy.
Conclusion
The diverse health needs of patients with EB emphasize the importance of individualized care and interventions, including photobiomodulation therapy, to alleviate pain and promote healing.
Does Rural Credit Participation Promote the Adoption of New Technologies by Family Farms? Evidence from Two Family Farm Demonstration Areas in China
Authors: Wang qianling , Gui li , Meng Qianyue , Wang Huichuan , Hu Jingwen 
Published: February 2025

Abstract

This study applies an asset portfolio investment framework to examine the impact of rural credit participation on the adoption of new agricultural technologies. Using field survey data from two family farm demonstration areas in China (Wuhan, Hubei Province, and Langxi, Anhui Province), the study employs the propensity score matching (PSM) method. The results show a significant positive relationship between rural credit and new technology adoption. Formal rural credit promotes the adoption of high-capital, low-risk technologies. In contrast, informal credit supports the adoption of low-capital, high-risk technologies. The effects of rural credit are stronger for large-scale family farms, which are better positioned to use financial resources for technological upgrades. Small-scale family farms benefit less in comparison. These findings highlight the role of financial mechanisms in agricultural modernization and emphasize the need for targeted rural financial policies to improve credit access and promote innovative technologies in family farming systems.
JEL: Q16; O33
From Quiet Quitting to Knowledge Hiding: Unraveling the Path through Employee Silence
Authors: Bari Muhammad Waseem 
Published: February 2025

Abstract

This research examines the relationship between quiet quitting strategies, i.e., organizational, relational, and work-life spillover, and knowledge-hiding behaviors. Drawing on social exchange theory, the study investigates how these quiet quitting strategies impact knowledge hiding, including evasive hiding, playing dumb, and rationalized hiding, where employee silence acts as a mediator. The study employed PLS-SEM and Smart PLS 4 and collected data from 466 IT professionals from China to establish that quiet quitting strategy and knowledge-hiding behaviors have direct and indirect relationships.
Results: Relational quitting and work-life spillover quitting have a significant impact on knowledge-hiding behaviors, however, organizational quitting has not a significant impact on evasive knowledge-hiding. Employee silence mediates these relationships, strengthening the impact of quiet quitting strategies on knowledge-holding. It emphasizes employee silence as the course of organizational quitting. IPMA also highlights the significance of employee silence in the current study model. Open employees’ communication channels help to prevent employees quiet quitting.
Effective Feedback in Medical and Health Science Education, in Malawi: Perceptions and Experiences
Authors: Thomson Emma , Mwambyale Tuntufye , Jones Linda 
Published: February 2025

Abstract

Background
Well-delivered feedback can positively influence learning behaviours and bring actual and desired performances closer together. This is especially important for patient safety in health professions education. Feedback only aids learning when used effectively and accepted by its recipients. Most current literature on effective feedback is from western perspectives that we cannot assume will be applicable to all cultures. This study seeks to contribute to discourses relating to feedback effectiveness, exploring concepts of optimizing it in a sub-Saharan healthcare education institution.
Methods
This qualitative study investigated student and lecturer perceptions and experiences of receiving and delivering feedback at College of Medicine (CoM), Malawi, and how stakeholders felt this influenced self-reported behavioural change in response.
Data was collected using semi-structured interviews, with students and lecturers, from across all faculties at CoM, and subsequently analysed sequentially for emerging themes using a grounded theory approach.
Results
Inductive data analysis resulted in eight interconnected themes: conception of feedback; affective dimension; thirst for feedback; need to achieve; feedback conversations; self-preservation; feedback relationships; students’ ideal feedback which included the desire for pastoral support. Several tensions also emerged from student data: Firstly, a yearning for feedback but experiencing corrective feedback as emotional assaults requiring action for self-preservation. Secondly a preference for receiving affirmative feedback, despite acknowledging that corrective feedback can lead to improved performance. Thirdly, participants valuing dialogue highly as opportunities to seek clarification/understanding/justify themselves, which was deemed less likely with senior faculty whose feedback was paradoxically greatly respected. Whilst students greatly valued feedback from senior faculty, due to cultural respect for hierarchy, they felt less able to engage in the dialogue they desired. Finally, both stakeholder groups equated hard work with success and that critical feedback was perceived as accusations of student laziness.
Conclusion
‘Best feedback practices’ articulated by students mostly aligned with western educational discourses but in contrast to international literature students desired lecturers to fulfil pastoral roles rather than simply provide feedback on tasks. Cultural expectations and respect for hierarchy needs to be considered when building evidence-based feedback in a Malawian context.