bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hdac1 and Hdac2 positively regulate Notch1 gain‐of‐function pathogenic signaling in committed osteoblasts of male mice
Authors: Torres Haydee M. , Hinojosa Leetoria , VanCleave Ashley M. , Rodezno Tania , Westendorf Jennifer J. , Tao Jianning 
Published: November 2023

AbstractBackgroundSkeletal development requires precise extrinsic and intrinsic signals to regulate processes that form and maintain bone and cartilage. Notch1 is a highly conserved signaling receptor that regulates cell fate decisions by controlling the duration of transcriptional bursts. Epigenetic molecular events reversibly modify DNA and histone tails by influencing the spatial organization of chromatin and can fine‐tune the outcome of a Notch1 transcriptional response. Histone deacetylase 1 and 2 (HDAC1 and HDAC2) are chromatin modifying enzymes that mediate osteoblast differentiation. While an HDAC1‐Notch interaction has been studied in vitro and in Drosophila, its role in mammalian skeletal development and disorders is unclear. Osteosclerosis is a bone disorder with an abnormal increase in the number of osteoblasts and excessive bone formation.MethodsHere, we tested whether Hdac1/2 contribute to the pathogenesis of osteosclerosis in a murine model of the disease owing to conditionally cre‐activated expression of the Notch1 intracellular domain in immature osteoblasts.ResultsImportantly, selective homozygous deletions of Hdac1/2 in osteoblasts partially alleviate osteosclerotic phenotypes (Col2.3kb‐Cre; TGRosaN1ICD/+; Hdac1flox/flox; Hdac2flox/flox) with a 40% decrease in bone volume and a 22% decrease in trabecular thickness in 4 weeks old when compared to male mice with heterozygous deletions of Hdac1/2 (Col2.3 kb‐Cre; TGRosaN1ICD/+; Hdac1flox/+; Hdac2flox/+). Osteoblast‐specific deletion of Hdac1/2 in male and female mice results in no overt bone phenotype in the absence of the Notch1 gain‐of‐function (GOF) allele.ConclusionsThese results provide evidence that Hdac1/2 contribute to Notch1 pathogenic signaling in the mammalian skeleton. Our study on epigenetic regulation of Notch1 GOF‐induced osteosclerosis may facilitate further mechanistic studies of skeletal birth defects caused by Notch‐related GOF mutations in human patients, such as Adams‐Oliver disease, congenital heart disease, and lateral meningocele syndrome.
Rare pyoderma gangrenosum correlated with systemic lupus erythematosus: A case report
Authors: Pourbagherian Omid , Javidi Kamran , Taghizadieh Mohammad , Jafarpour Mehdi 
Published: November 2023

Key Clinical MessageThe simultaneous occurrence of pyoderma gangrenosum and systemic lupus erythematosus is exceedingly rare and poses diagnostic challenges due to the similarity of skin manifestations in both conditions. The exact relationship between the two conditions remains unclear; however, it is hypothesized that immune dysregulation and neutrophilic infiltration may play a role in the development of pyoderma gangrenosum in SLE patients. Clinicians should be vigilant in recognizing such uncommon associations to ensure prompt and appropriate management.
Finger prosthesis: A novel way to restore the form, function, and esthetics
Authors: Somayaji Nagaveni S. , Sinha Pallawi , Sharan Jitendra , Rajaguru Jagadish Prasad , Marya Anand 
Published: November 2023

Key Clinical MessageA well‐customized prosthesis with a life‐like esthetic and function are the primary determining factors for its acceptance and success. RTV silicones can prove to be very effective and useful in fabricating such prosthesis.AbstractComplete or partial finger amputations impact an individual's psychological and physical well‐being and are considered the most frequently observed pattern of hand loss. A customized prosthesis offers the patient rehabilitative, functional, and psychological advantages. Retention is the key to the success of such prosthetic restoration. The present case report describes a novel technique that utilizes passive vacuum fit and mechanical retention to restore controlled function movements as a metal wire framework. The procedure was economical, most importantly, produced life‐like anatomy of the missing digit and restored the function to some extent.
Evaluation of radon‐induced radiation risk in indoor environments
Authors: Elek Nedime İrem , Çam Nebiye Füsun , Öztürk Buket Canbaz 
Published: November 2023

AbstractIn general people spend most of their time in enclosed environments, therefore, they are exposed to indoor radon and its progeny. The paper discusses the variables influencing indoor radon levels. It assesses the correlation between indoor radon levels with temperature, humidity, and pressure, as well as seasonal variables. Radon activity concentrations were measured in Ege University's laboratories and Classroom using a digital radon gas detector. After statistical analysis, the results were compared to committee‐permissible limit levels (ICRP, WHO, and EU). It was found that the average radon activity concentrations were below the recommended 300 Bq m–3 limit value (EU), ranging from 58.4 to 216.0 Bq m–3. This study emphasized the significance of measuring and assessing the indoor radon levels in laboratories and classrooms in particular, in order to safeguard the students and faculty members from radon‐related health issues.
Prospective study of cenobamate on cognition, affectivity, and quality of life in focal epilepsy
Authors: Catalán‐Aguilar Judit , Hampel Kevin G. , Cano‐López Irene , Garcés Mercedes , Lozano‐García Alejandro , Tormos‐Pons Paula , González‐Bono Esperanza , Villanueva Vicente 
Published: November 2023

AbstractObjectiveCenobamate is a recently approved antiseizure medication that proved to be safe and effective in randomized controlled trials. However, little is known about its impact on some areas frequently affected by epilepsy. For this reason, we explored the effects of cenobamate on cognitive performance, as well as on negative affectivity and quality of life in a sample of patients with drug‐resistant epilepsy.MethodsTwo prospective cohort studies were carried out. In Study 1, 32 patients (22 men and 10 women) underwent a baseline (T0) and a short‐term (T1) neuropsychological assessment after 3 months of cenobamate administration. In Study 2, 22 patients (16 men and 6 women) from the T1 sample also underwent a baseline and a follow‐up evaluation (T2) 6 months after T0.ResultsNo significant differences were found in cognitive variables, negative affectivity, and quality of life either in Study 1 or Study 2. Similarly, based on the reliable change index, it was found that most patients showed no changes in these variables.SignificanceThese results suggest that cenobamate is a safe antiseizure medication in terms of cognition, negative affectivity, or quality of life since no adverse events have been found after 3 and 6 months of treatment.Plain Language SummaryCenobamate is a new antiseizure medication. In patients with epilepsy, cenobamate seems to not affect cognition, anxiety, depression, or quality of life.
The effect of environmental centralisation on productivity: Evidence from an administrative reform in China
Authors: Li Weibing , Yang Yongwen 
Published: November 2023

AbstractThis paper adopts a novel institutional perspective—environmental centralisation—to examine its impact on firm productivity in developing countries. In 2008, China's State Environmental Protection Administration was upgraded to the Ministry of Environmental Protection (MEP). This administrative reform strengthened the central government's ability to protect the environment, enabling it to address more environmental affairs and concentrate on environmental rights. Based on the quasi‐natural experiment of the establishment of the MEP, this study takes a new perspective on the impact of environmental centralisation on productivity. The difference‐in‐difference‐in‐difference approach is used for the first time to estimate the impact of environmental centralisation on productivity. We find that environmental centralisation caused by the establishment of the MEP significantly increased the productivity of heavily polluting companies, and a series of robustness tests confirm that the results are credible. We identify three possible mechanisms, namely, facilitating innovation, curbing rent‐seeking behaviour and inhibiting over‐investments. The policy implication of this study is that the effectiveness of environmental policy depends not only on the policy itself but also on the allocation of environmental rights among different levels of government.
Seasonal forecast of tropical cyclones in the Southwest Pacific Ocean
Authors: van Vloten Sara O. , Pozo Andrea , Cagigal Laura , Méndez Fernando J. 
Published: November 2023

AbstractPredictions of tropical cyclone (TC) activity have been a topic of recurrent interest and research in the past. Here we utilize reanalysis datasets of sea surface temperature (SST) and mixed layer depth (MLD) to build a statistical seasonal forecasting model that produces outlooks of expected TC counts in the region of the Southwest Pacific (SWP). Nevertheless, the model applicability can be extended to other regions and basins. A novel TC predictor index is developed at the daily scale and used to obtain an objective classification of synoptic weather patterns. This classification has been performed by clustering the daily index predictor fields, previously transformed into principal components, using a K‐mean algorithm. As a result, 49 daily weather types (DWTs) are presented which inform about the mean representative features and spatial patterns of both predictor and predictand variables. Thus, statistical relationships between TC activity and nonlinear combinations of predictor variables are found to assign daily rates of expected TCs. The cluster‐based model is calibrated from 1982 to 2019 and validated by recent TC season observations, demonstrating the operational application using ensembles of long‐term predictions in the Southwest Pacific. Results have shown which synoptic types of SST and MLD are favourable to cyclogenesis and activity, with additional information related to concurrent sea level pressure and precipitation synoptic patterns, as well as seasonal and interannual climate variability.
Hierarchical critic learning optimal control for lower limb exoskeleton robots with prescribed constraints
Authors: Sun Yaohui , Hu Jiangping , Peng Zhinan , Ghosh Bijoy K. 
Published: November 2023

AbstractThis paper presents a hierarchical critic learning optimal control (H‐CLOC) strategy for a lower limb exoskeleton system with prescribed state constraints. The prescribed state constraints of the exoskeleton are mathematically modeled by means of a state error transition function, which transforms the constrained state into an equivalent new unconstrained state. A hierarchical framework including a high‐level motion trajectory planner and a low‐level critic learning (CL) controller is proposed for optimal control of the exoskeleton. The estimation of human motion intentions is achieved using dynamic movement primitives (DMPs), which are capable of accurately replicating the original training trajectories during motion. The CL algorithm is implemented on the transformed unconstrained system while enhancing the learning capability of the proposed algorithm for the exoskeleton. The optimal control with prescribed constraints is analyzed for stability using the Lyapunov theorem. Finally, simulation results validate the effectiveness of the proposed H‐CLOC strategy for lower limb exoskeletons.
Electroanalytical Investigation of 2‐(2‐Methylbenzyl)‐4(7)‐phenyl‐1H‐benzo[d]imidazole, a Novel Benzimidazole Derivative
Authors: Ağın Fatma , Öztürk Gökçe , Doğan İnci S. , Kahveci Bahittin , Kul Dilek 
Published: November 2023

AbstractElectrochemical analysis of 2‐(2‐methylbenzyl)‐4(7)‐phenyl‐1‐H‐benzo[d]imidazole (MPB), a new benzimidazole derivative with tyrosinase inhibitory effect and antioxidant activity, was performed in this study. The electro‐oxidation behavior of MPB was investigated by cyclic voltammetry (CV), differential pulse stripping voltammetry (DPSV), and square wave voltammetry (SWV) on glassy carbon electrode. The oxidation process of MPB showed an irreversible behavior. The highest peak current was obtained in 0.5 M H2SO4 containing 10 % methanol and used as the supporting electrolyte. As a result of the calibration study, two linear responses in the range of 0.06–1.00 μM and 2.00–100 μM were obtained with a detection limit of 10.4 nM by DPSV. Linearity for SWV was between 0.1 and 10.0 μM with a detection limit of 29.3 nM. Precision of the methods was obtained as relative standard deviation (RSD) and was below 2.0 %. Developed methods were applied for the recovery of MPB from spiked serum samples. Accordingly, recoveries of 100.39 % for DPSV and 100.07 % for SWV were obtained with RSD below 0.48 %. MPB was stable for at least 2 weeks as a result of retaining 97 % of the peak current when stored at +4 °C. Finally, the possible responsible group in MPB oxidation was proposed.
Montmorillonite K10 Clay Accelerated One‐Pot Multicomponent Synthesis of Substituted 2‐Amino‐3,5‐dicarbonitrile‐6‐phenylsulfanylpyridines: Experimental and Theoretical Investigation
Authors: Sharma Pragati , Sinha Pragya 
Published: November 2023

Abstract2‐Amino‐3,5‐dicarbonitrile‐6‐phenylsulfanylpyridines occupy a prominent place among the physiologically and biologically active scaffolds owing to their therapeutic applications in the treatment of Parkinson's disease, asthma, urinary incontinence, kidney disease, the Hepatitis B infections, Creutzfeldt–Jacob disease, cancer, hypoxia/ischemia and epilepsy. A one‐pot, multicomponent condensation of aldehydes, malononitrile, and thiophenol catalyzed by Montmorillonite K10 clay afforded 2‐amino‐3,5‐dicarbonitrile‐6‐phenylsulfanylpyridines in good to excellent yields. All the products could be well characterized on the basis of spectral studies. The catalyst could be recycled three times without affecting the yields and even in the fourth recycling of the catalyst, the yield decreases marginally. This approach manifests environmentally benign feasibility in contexts of enhanced yields, milder conditions, short reaction times, and reusability of the catalyst. A theoretical study of a synthesized compound at the B3LYP/6–31 + G (d) level is additionally reported. Following the computation of the fundamental vibrational wavenumbers and intensities, it became evident that the observed and calculated wavenumbers were in perfect accord. Employing the same DFT level, Frontier molecular orbital (HOMO‐LUMO) energies, global reactivity descriptors, and molecular electrostatic potential were estimated for assessing the reactivity and possible sites for electrophilic and nucleophilic molecules. The outcomes prove that the compound exhibits significant kinetic stability.
Looking for New [1,2,4,5]Tetrazines to Produce 99mTc‐Labelled Derivatives, with a Suitable Lipophilicity Balance for Use in Bioorthogonal Reactions
Authors: Rodríguez Gonzalo , Fernández Marcelo , Cabrera Mirel , Tassano Marcos , Cabral Pablo , Couto Marcos , Cerecetto Hugo , García. María Fernanda 
Published: November 2023

AbstractThe reaction between [1,2,4,5]tetrazines with trans‐cyclooctene through the cycloaddition process has been described as a powerful tool in bioorthogonal processes. Specifically the diagnostic with fluorescent‐ and radio‐labelled [1,2,4,5]tetrazines and pre‐targeted trans‐cyclooctene modified‐antibody has been used by us for this purpose. Our previously developed 99mTc‐radiolabelled [1,2,4,5]tetrazine‐derivatives porting 6‐hydrazinonicotinyl‐chelator displayed some limitations related to its unappropriated biodisposal. Herein, we explored [1,2,4,5]tetrazines porting other 99mTc‐coordination‐moieties, 1,4,8,11‐tetrazacyclotetradecanyl and diethylenetriaminepentaacetyl, as potential hydrophilic functions. Some of the new modified [1,2,4,5]tetrazines were able to coordinate efficiently the radionuclide generating 99mTc‐counterparts that reacted with trans‐cyclooctene modified‐bevacizumab and recognized cells that overexpress vascular endothelial growth factor (VEGF). Additionally, the in vivo 99mTc‐counterparts‐biodistributions were studied, on the desired organs, and they resulted dependent of lipophilicity.
Large Enhancement of Polarization in a Layered Hybrid Perovskite Ferroelectric Semiconductor via Molecular Engineering
Authors: Wang Na , Ding Ning , Xu Ze‐Jiang , Luo Wang , Li Hua‐Kai , Shi Chao , Ye Heng‐Yun , Dong Shuai , Miao Le‐Ping 
Published: November 2023

AbstractSwitchable spontaneous polarization is the vital property of ferroelectrics, which leads to other key physical properties such as piezoelectricity, pyroelectricity, and nonlinear optical effects, etc. Recently, organic–inorganic hybrid perovskites with 2D layered structure have become an emerging branch of ferroelectric materials. However, most of the 2D hybrid ferroelectrics own relatively low polarizations (<15 µC cm−2). Here, a strategy to enhance the polarization of these hybrid perovskites by using ortho‐, meta‐, para‐halogen substitution is developed. Based on (benzylammonium)2PbCl4 (BZACL), the para‐chlorine substituted (4‐chlorobenzylammonium)2PbCl4 (4‐CBZACL) ferroelectric semiconductor shows a large spontaneous polarization (23.3 µC cm−2), which is 79% larger than the polarization of BZACL. This large enhancement of polarization is successfully explained via ab initio calculations. The study provides a convenient and efficient strategy to promote the ferroelectric property in the hybrid perovskite family.
The impacts of U.S. right‐to‐work laws on free riding, unionization, and compensation
Authors: Bono‐Lunn Dillan 
Published: November 2023

AbstractRight‐to‐work (RTW) laws are theorized to precipitate union decline by inducing free riding among union‐covered workers; however, this relationship has not been empirically explored. This paper uses recent policy variation and Current Population Survey (CPS) data to identify the impact of state RTW laws on union status (free riding, membership, and coverage), wages, and benefits among private and public sector workers. Doubly robust difference‐in‐difference estimates suggest RTW laws are associated with substantial increases in free riding, decreases in union membership, and decreases in union coverage. Thus, RTW laws weaken unions through combinations of increased free riding and decreased coverage, though effects vary by state and sector. While RTW laws are not associated with changes in wage differentials, state fixed effects estimates suggest that unions provide some excludable advantages to members in the face of RTW legislation, through higher earnings (union wage premium) and fringe benefits (union benefit premium).
Elucidating the morphology and ecology of Eoandromeda octobrachiata from the Ediacaran of South Australia
Authors: Botha Tory L. , Sherratt Emma , Droser Mary L. , Gehling Jim G. , García‐Bellido Diego C. 
Published: November 2023

AbstractEoandromeda octobrachiata is a poorly understood Ediacaran organism, with spiral octoradial arms, found in South Australia and South China. The informal Nilpena member of the Rawnsley Quartzite, Flinders Ranges in South Australia preserves more than 200 specimens of Eoandromeda. Here we use the novel application of rotational geometric morphometrics together with palaeoenvironmental information to provide a better insight into their palaeobiology and ecology, and to address conflicting hypotheses regarding mode of life and taxonomic affinity. We find that Eoandromeda probably had a radially symmetrical shape in life, was cone shaped and had a high relief off the microbial mat. Analysis of the symmetric and asymmetric shape components showed that they deform strongly in the direction of palaeocurrent, therefore they are thought to be made of a flexible material. Almost all specimens are compressed flat. Specimens that appear to have not fully collapsed support the idea that Eoandromeda was probably cone shaped and suggest that they possibly collapsed spirally. Our shape analysis, along with observed morphological features, support a benthic rather than pelagic mode of life. Morphological and ecological inconsistencies do not fully support the hypothesis of a Ctenophora taxonomic affinity.
Establishment and application of a loop‐mediated isothermal amplification−lateral flow dipstick (LAMP–LFD) method for detecting Clostridium piliforme
Authors: Tao Junhao , Yan Huiqiong , Chen Sisi , Du Jiangtao , Zhou Shasang , Guo Honggang , Lu Lingqun , Fang Jie , Jin Xiaoyin , Wang Zhiyuan , Ying Huazhong , Han Wei , Dai Fangwei 
Published: November 2023

AbstractBackgroundClostridium piliforme (causative agent of Tyzzer disease) infects various animals, including primates, and hence a threat to animal and human health worldwide. At present, it is detected using traditional methods, such as path morphology, polymerase chain reaction and enzyme‐linked immunosorbent assay. Therefore, it is necessary to develop convenient, efficient visual molecular biological methods for detecting C. piliforme.ObjectivesTo establish a method with good specificity, high sensitivity and simple operation for the detection of C. piliforme.MethodsIn this study, we designed internal and external primers based on the conserved 23S rRNA region of C. piliforme to develop a biotin‐labelled diarrhoea‐suffered loop‐mediated isothermal amplification (LAMP) system for detecting of C. piliforme and assessed the specificity, sensitivity and repeatability of the LAMP system.ResultsThe LAMP system did not exhibit cross‐reactivity with 24 other common pathogenic species, indicating that it had good specificity. The minimum concentration of sensitivity was 1 × 10−7 ng/μL. Mouse models (Meriones unguiculatus) of Tyzzer disease were established and a LAMP−lateral flow dipstick (LAMP–LFD) was developed for detecting C. piliforme. The detection rate of C. piliforme was 5.08% in clean‐grade animals and 9.96% in specific‐pathogen‐free‐grade animals from Jiangsu, Zhejiang and Shanghai. In addition, the detection rates of C. piliforme were 10.1%, 8.6% and 20%, in animals from Hangzhou, Wenzhou and Shaoxing, respectively. The detection rate of C. piliforme was higher in experimental animals used in schools than in those used in companies and research institutes.ConclusionsThe LAMP–LFD method established in this study can be used to detect C. piliforme in animals handled in laboratory facilities of universities, pharmaceutical enterprises and research and development institutions.
Wilson loop correlators at strong coupling in $$ \mathcal{N} $$ = 2 quiver gauge theories
Authors: Pini Alessandro , Vallarino Paolo 
Published: November 2023

Abstract
We consider 4-dimensional $$ \mathcal{N} $$
N
= 2 superconformal quiver theories with SU(N)M gauge group and bi-fundamental matter and we evaluate correlation functions of n coincident Wilson loops in the planar limit of the theory. Exploiting specific untwisted/twisted combinations of these operators and using supersymmetric localization, we are able to resum the whole perturbative expansion and find exact expressions for these correlators that are valid for all values of the ’t Hooft coupling. Moreover, we analytically derive the leading strong coupling behaviour of the correlators, showing that they obey a remarkable simple rule. Our analysis is complemented by numerical checks based on a Padé resummation of the perturbative series.
The chiral condensate of Nf = 2 + 1 QCD from the spectrum of the staggered Dirac operator
Authors: Bonanno Claudio , D’Angelo Francesco , D’Elia Massimo 
Published: November 2023

Abstract
We compute the chiral condensate of 2 + 1 QCD from the mode number of the staggered Dirac operator, performing controlled extrapolations to both the continuum and the chiral limit. We consider also alternative strategies, based on the quark mass dependence of the topological susceptibility and of the pion mass, and obtain consistent results within errors. Results are also consistent with phenomenological expectations and with previous numerical determinations obtained with different lattice discretizations.