bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influencing factors of knowledge proficiency of general practitioners in rural China for esophageal cancer prevention and treatment: a cross-sectional study
Authors: Zhang Jinjia , Wu Huadong , Wang Rongying , Zhang Min 
Published: February 2024

AbstractBackground:This study aims to investigate the knowledge of rural general practitioners (GPs) in esophageal cancer (EC) prevention and treatment in China and analyze relevant influencing factors, so as to improve the ability of rural GPs in EC prevention and treatment.Methods:This cross-sectional study was conducted from November 5, 2021, to November 20, 2021. A self-designed questionnaire was used to conduct an online survey. Multivariable logistic regression models were used to identify the influencing factors of knowledge proficiency of GPs in rural China for EC prevention and treatment.Results:This study included 348 participants from 12 rural areas in Hebei Province. The mean accuracy rate on all question items was 42.3% ± 10.67%. Sex (OR = 2.870, 95% CI: 1.519–5.423), educational level (OR = 3.256, 95% CI: 1.135–9.339), and comprehension of clinical practice guidelines for EC (OR = 4.305, 95% CI: 2.023–9.161) were significant predictors for GPs’ knowledge proficiency of EC prevention and treatment (P< 0.05).Conclusions:The study indicated that knowledge proficiency of rural GPs of EC prevention and control still awaits to be improved. Sex, educational level, and comprehension of clinical practice guidelines for EC were significant predictors for their proficiency.
Ethnic minority MPs as reputational shields? How Western European political parties respond to public opinion shifts on immigration policy
Authors: van de Wardt Marc , Sobolewska Maria , English Patrick 
Published: February 2024

AbstractIn recent decades, representation of ethnic minorities increased significantly across Europe, while concurrently many political parties moved to the right on multiculturalism and immigration, a seeming paradox. We explain it by arguing that often it is the same parties that move to the right and simultaneously increase representation. They use this dual strategy in an attempt to positionally converge to the median voter, where the increased minority representation acts as a reputational shield to prevent allegations of intolerance. Looking at parliaments of eight European countries between 1990 and 2015, we find that parties that shifted to the right in response to a public mood swing to the right are indeed significantly more likely to bring more ethnic minority politicians into parliament. This has important implications for the literature on descriptive representation and party platform change.
An enhanced wideband tracking method for characteristic modes
Authors: Huang Chao , Guo Chenjiang , Ma Xia , Yuan Yi , Ding Jun 
Published: February 2024

AbstractAn enhanced wideband tracking method for characteristic modes (CMs) is investigated in this paper. The method consists of three stages, and its core tracking stage (CTS) is based on a classical eigenvector correlation-based algorithm. To decrease the tracking time and eliminate the crossing avoidance (CRA), we append a commonly used eigenvalue filter (EF) as the preprocessing stage and a novel postprocessing stage to the CTS. The proposed postprocessing stage can identify all CRA mode pairs by analyzing their trajectory and correlation characteristics. Subsequently, it can predict corresponding CRA frequencies and correct problematic qualities rapidly. Considering potential variations in eigenvector numbers at consecutive frequency samples caused by the EF, a new execution condition for the adaptive frequency adjustment in the CTS is introduced. Finally, CMs of a conductor plate and a fractal structure are investigated to demonstrate the performance of the proposed method, and the obtained results are discussed.
Interdependencies Between Wildfire‐Induced Alterations in Soil Properties, Near‐Surface Processes, and Geohazards
Authors: Vahedifard Farshid , Abdollahi Masood , Leshchinsky Ben A. , Stark Timothy D. , Sadegh Mojtaba , AghaKouchak Amir 
Published: February 2024

AbstractThe frequency, severity, and spatial extent of destructive wildfires have increased in several regions globally over the past decades. While direct impacts from wildfires are devastating, the hazardous legacy of wildfires affects nearby communities long after the flames have been extinguished. Post‐wildfire soil conditions control the persistence, severity, and timing of cascading geohazards in burned landscapes. The interplay and feedback between geohazards and wildfire‐induced changes to soil properties, land cover conditions, and near‐surface and surface processes are still poorly understood. Here, we synthesize wildfire‐induced processes that can affect the critical attributes of burned soils and their conditioning of subsequent geohazards. More specifically, we discuss the state of knowledge pertaining to changes in mineralogical, hydraulic, mechanical, and thermal properties of soil due to wildfire with a focus on advances in the past decade. We identify how these changes in soil properties alter evapotranspiration, interception, sediment transport, infiltration, and runoff. We then link these alterations to the evolution of different geohazards, including dry raveling, erosion, rockfalls, landslides, debris flows, and land subsidence. Finally, we identify research gaps and future directions to advance knowledge on how wildfires control the evolution of various earth surface processes and geohazards over time.
KNMI'23 Climate Scenarios for the Netherlands: Storyline Scenarios of Regional Climate Change
Authors: van der Wiel Karin , Beersma Jules , van den Brink Henk , Krikken Folmer , Selten Frank , Severijns Camiel , Sterl Andreas , van Meijgaard Erik , Reerink Thomas , van Dorland Rob 
Published: February 2024

AbstractThis paper presents the methodology for the construction of the KNMI'23 national climate scenarios for the Netherlands. We have developed six scenarios, that cover a substantial part of the uncertainty in CMIP6 projections of future climate change in the region. Different sources of uncertainty are disentangled as much as possible, partly by means of a storyline approach. Uncertainty in future emissions is covered by making scenarios conditional on different SSP scenarios (SSP1‐2.6, SSP2‐4.5, and SSP5‐8.5). For each SSP scenario and time horizon (2050, 2100, 2150), we determine a global warming level based on the median of the constrained estimates of climate sensitivity from IPCC AR6. The remaining climate model uncertainty of the regional climate response at these warming levels is covered by two storylines, which are designed with a focus on the annual and seasonal mean precipitation response (a dry‐trending and wet‐trending variant for each SSP). This choice was motivated by the importance of future water management to society. For users with specific interests we provide means how to account for the impact of the uncertainty in climate sensitivity. Since CMIP6 GCM data do not provide the required spatial detail for impact modeling, we reconstruct the CMIP6 responses by resampling internal variability in a GCM‐RCM initial‐condition ensemble. The resulting climate scenarios form a detailed storyline of plausible future climates in the Netherlands. The data can be used for impact calculations and assessments by stakeholders, and will be used to inform policy making in different sectors of Dutch society.
The Chemical Effect of Increased Water Vapor From the Hunga Tonga‐Hunga Ha'apai Eruption on the Antarctic Ozone Hole
Authors: Wohltmann Ingo , Santee Michelle L. , Manney Gloria L. , Millán Luis F. 
Published: February 2024

AbstractThe eruption of the Hunga Tonga‐Hunga Ha'apai volcano on 15 January 2022 was one of the most explosive eruptions of the last decades. The amount of water vapor injected into the stratosphere was unprecedented in the observational record, increasing the stratospheric water vapor burden by about 10%. Using model runs from the ATLAS chemistry and transport model and Microwave Limb Sounder (MLS) satellite observations, we show that while 20%–40% more water vapor than usual was entrained into the Antarctic polar vortex in 2023 as it formed, the direct chemical effect of the increased water vapor on Antarctic ozone depletion in June through October was minor (less than 4 DU). This is because low temperatures in the vortex, as occur every year in the Antarctic, limit water vapor to the saturation pressure and thus reset any anomalies through the process of dehydration before they can affect ozone loss.
Plasma Observations in the Distant Magnetotail During Intervals of Northward IMF
Authors: Mooney M. K. , Milan S. E. , Bower G. E. 
Published: February 2024

AbstractWe examine a 6‐day traversal of the magnetotail by the ARTEMIS satellites during an interval of prolonged northward IMF. The electrostatic analyzer (ESA) onboard the ARTEMIS spacecraft measures high ion and electron fluxes at approximately 60 RE downtail in regions of the magnetotail which would normally be the magnetotail lobe, containing open flux evacuated of plasma. We interpret these observations as trapped plasma on closed magnetic flux indicating that the magnetotail is closed or partially closed but extends at least as far as ∼60 RE downtail. We find that the occurrence of plasma in the magnetotail and the closure of the magnetosphere results in distinct changes to the magnetotail structure including a reduction in the magnetic field strength and pressure as well as a narrowing of the tail by approximately 20 RE.
Limited Evidence for a Microbial Signal in Ground‐Level Smoke Plumes
Authors: Gering Sarah M. , Sullivan Amy P. , Kreidenweis Sonia M. , McMurray Jill A. , Fierer Noah 
Published: February 2024

AbstractRecent studies have suggested that microbial aerosolization in wildfire smoke is an understudied source of microbes to the atmosphere. Wildfire smoke can travel thousands of kilometers from its source with the potential to facilitate the transport of microbes, including microbes that can have far‐reaching impacts on human or ecosystem health. However, the relevance of longer‐range detection of microbes in smoke plumes remains undetermined, as previous studies have mainly focused on analyses of bioaerosols collected adjacent to or directly above wildfires. Therefore, we investigated whether wildfire smoke estimated to originate >30 km from different wildfire sources would contain detectable levels of bacterial and fungal DNA at ground level, hypothesizing that smoke‐impacted air would harbor greater amounts and a distinct composition of microbes as compared to ambient air. We used cultivation‐independent approaches to analyze 150 filters collected over time from three sampling locations in the western United States, of which 34 filters were determined to capture wildfire smoke events. Contrary to our hypothesis, smoke‐impacted samples harbored lower amounts of microbial DNA. Likewise, there was a limited signal in the composition of the microbial assemblages detected in smoke‐affected samples as compared to ambient air, but we did find that changes in humidity were associated with temporal variation in the composition of the bacterial and fungal bioaerosols. With our study design, we were unable to detect a robust and distinct microbial signal in ground‐level smoke originating from distant wildfires.
Anzali Wetland Crisis: Unraveling the Decline of Iran's Ecological Gem
Authors: Mahdian M. , Noori R. , Salamattalab M. M. , Heggy E. , Bateni S. M. , Nohegar A. , Hosseinzadeh M. , Siadatmousavi S. M. , Fadaei M. R. , Abolfathi S. 
Published: February 2024

AbstractThe wetland loss rate in Iran is faster than the global average. Comprehending the shrinkage rate in Iranian wetlands and identifying the underlying drivers of these changes is essential for safeguarding their ecosystems' health and services. This study proposes a novel gray‐box modeling framework to quantify the effects of climate change and anthropogenic activities on the wetlands, by combining process‐based and machine learning models. The developed model is utilized to project the Anzali coastal wetland shrinkage by simulating the complex interaction between the meteorological, hydrological, anthropogenic and sea water level characteristics, and the changes in wetland water surface area. Our framework aggregates Soil and Water Assessment Tool model, the 12 General Circulation Models of the Coupled Model Intercomparison Project Phase 6, Landsat imagery, and the Long Short‐Term Memory model to project the shrinkage of the wetland till 2100. A comprehensive range of climate and Land Use/Cover change scenarios are analyzed. The results show that wetland will seasonally desiccate in 2058, mainly due to increasing air temperature, reduction in precipitation and inflow, excessive sediment loading to the wetland, and decline in the Caspian Sea level. For optimistic scenarios, where no changes in the Caspian Sea level is considered, the wetland will gradually diminish and become a seasonal waterbody by 2100. The outcomes of this study highlight that the Anzali wetland desiccation has profound implications for the regional‐scale ecological balance, ecosystem health and function, public health, and local economy. Robust environmental interventions and sustainable development strategies are urgently needed to mitigate the detrimental impacts of climate and anthropogenic drivers on the wetland.
Exploiting epigenetic targets to overcome taxane resistance in prostate cancer
Authors: Cevatemre Buse , Bulut Ipek , Dedeoglu Beyza , Isiklar Arda , Syed Hamzah , Bayram Ozlem Yedier , Bagci-Onder Tugba , Acilan Ceyda 
Published: February 2024

AbstractThe development of taxane resistance remains a major challenge for castration resistant prostate cancer (CR-PCa), despite the effectiveness of taxanes in prolonging patient survival. To uncover novel targets, we performed an epigenetic drug screen on taxane (docetaxel and cabazitaxel) resistant CR-PCa cells. We identified BRPF reader proteins, along with several epigenetic groups (CBP/p300, Menin-MLL, PRMT5 and SIRT1) that act as targets effectively reversing the resistance mediated by ABCB1. Targeting BRPFs specifically resulted in the resensitization of resistant cells, while no such effect was observed on the sensitive compartment. These cells were successfully arrested at the G2/M phase of cell cycle and underwent apoptosis upon BRPF inhibition, confirming the restoration of taxane susceptibility. Pharmacological inhibition of BRPFs reduced ABCB1 activity, indicating that BRPFs may be involved in an efflux-related mechanism. Indeed, ChIP-qPCR analysis confirmed binding of BRPF1 to the ABCB1 promoter suggesting direct regulation of the ABCB1 gene at the transcriptional level. RNA-seq analysis revealed that BRPF1 knockdown affects the genes enriched in mTORC1 and UPR signaling pathways, revealing potential mechanisms underlying its functional impact, which is further supported by the enhancement of taxane response through the combined inhibition of ABCB1 and mTOR pathways, providing evidence for the involvement of multiple BRPF1-regulated pathways. Beyond clinical attributes (Gleason score, tumor stage, therapy outcome, recurrence), metastatic PCa databases further supported the significance of BRPF1 in taxane resistance, as evidenced by its upregulation in taxane-exposed PCa patients.
HNF4α ubiquitination mediated by Peli1 impairs FAO and accelerates pressure overload-induced myocardial hypertrophy
Authors: Hou Yuxing , Shi Pengxi , Du Haiyang , Zhu Chenghao , Tang Chao , Que Linli , Zhu Guoqing , Liu Li , Chen Qi , Li Chuanfu , Shao Guoqiang , Li Yuehua , Li Jiantao 
Published: February 2024

AbstractImpaired fatty acid oxidation (FAO) is a prominent feature of metabolic remodeling observed in pathological myocardial hypertrophy. Hepatocyte nuclear factor 4alpha (HNF4α) is closely associated with FAO in both cellular processes and disease conditions. Pellino 1 (Peli1), an E3 ligase containing a RING-like domain, plays a crucial role in catalyzing polyubiquitination of various substrates. In this study, we aimed to investigate the involvement of HNF4α and its ubiquitination, facilitated by Peli1, in FAO during pressure overload-induced cardiac hypertrophy. Peli1 systemic knockout mice (Peli1KO) display improved myocardial hypertrophy and cardiac function following transverse aortic constriction (TAC). RNA-seq analysis revealed that changes in gene expression related to lipid metabolism caused by TAC were reversed in Peli1KO mice. Importantly, both HNF4α and its downstream genes involved in FAO showed a significant increase in Peli1KO mice. We further used the antagonist BI6015 to inhibit HNF4α and delivered rAAV9-HNF4α to elevate myocardial HNF4α level, and confirmed that HNF4α inhibits the development of cardiac hypertrophy after TAC and is essential for the enhancement of FAO mediated by Peli1 knockout. In vitro experiments using BODIPY incorporation and FAO stress assay demonstrated that HNF4α enhances FAO in cardiomyocytes stimulated with angiotension II (Ang II), while Peli1 suppresses the effect of HNF4α. Mechanistically, immunoprecipitation and mass spectrometry analyses confirmed that Peli1 binds to HNF4α via its RING-like domain and promotes HNF4α ubiquitination at residues K307 and K309. These findings shed light on the underlying mechanisms contributing to impaired FAO and offer valuable insights into a promising therapeutic strategy for addressing pathological cardiac hypertrophy.
The molecular interaction pattern of lenvatinib enables inhibition of wild-type or kinase-mutated FGFR2-driven cholangiocarcinoma
Authors: Spahn Stephan , Kleinhenz Fabian , Shevchenko Ekaterina , Stahl Aaron , Rasen Yvonne , Geisler Christine , Ruhm Kristina , Klaumuenzer Marion , Kronenberger Thales , Laufer Stefan A. , Sundberg-Malek Holly , Bui Khac Cuong , Horger Marius , Biskup Saskia , Schulze-Osthoff Klaus , Templin Markus , Malek Nisar P. , Poso Antti , Bitzer Michael 
Published: February 2024

AbstractFibroblast growth factor receptor (FGFR)−2 can be inhibited by FGFR-selective or non-selective tyrosine kinase inhibitors (TKIs). Selective TKIs are approved for cholangiocarcinoma (CCA) with FGFR2 fusions; however, their application is limited by a characteristic pattern of adverse events or evocation of kinase domain mutations. A comprehensive characterization of a patient cohort treated with the non-selective TKI lenvatinib reveals promising efficacy in FGFR2-driven CCA. In a bed-to-bench approach, we investigate FGFR2 fusion proteins bearing critical tumor-relevant point mutations. These mutations confer growth advantage of tumor cells and increased resistance to selective TKIs but remain intriguingly sensitive to lenvatinib. In line with clinical observations, in-silico analyses reveal a more favorable interaction pattern of lenvatinib with FGFR2, including an increased flexibility and ligand efficacy, compared to FGFR-selective TKIs. Finally, the treatment of a patient with progressive disease and a newly developed kinase mutation during therapy with a selective inhibitor results in a striking response to lenvatinib. Our in vitro, in silico, and clinical data suggest that lenvatinib is a promising treatment option for FGFR2-driven CCA, especially when insurmountable adverse reactions of selective TKIs or acquired kinase mutations occur.
Broad protection against clade 1 sarbecoviruses after a single immunization with cocktail spike-protein-nanoparticle vaccine
Authors: Halfmann Peter J. , Loeffler Kathryn , Duffy Augustine , Kuroda Makoto , Yang Jie E. , Wright Elizabeth R. , Kawaoka Yoshihiro , Kane Ravi S. 
Published: February 2024

AbstractThe 2002 SARS outbreak, the 2019 emergence of COVID-19, and the continuing evolution of immune-evading SARS-CoV-2 variants together highlight the need for a broadly protective vaccine against ACE2-utilizing sarbecoviruses. While updated variant-matched formulations are a step in the right direction, protection needs to extend beyond SARS-CoV-2 and its variants to include SARS-like viruses. Here, we introduce bivalent and trivalent vaccine formulations using our spike protein nanoparticle platform that completely protect female hamsters against BA.5 and XBB.1 challenges with no detectable virus in the lungs. The trivalent cocktails elicit highly neutralizing responses against all tested Omicron variants and the bat sarbecoviruses SHC014 and WIV1. Finally, our 614D/SHC014/XBB trivalent spike formulation completely protects human ACE2-transgenic female hamsters against challenges with WIV1 and SHC014 with no detectable virus in the lungs. Collectively, these results illustrate that our trivalent protein-nanoparticle cocktail can provide broad protection against SARS-CoV-2-like and SARS-CoV-1-like sarbecoviruses.
Functional nanoporous graphene superlattice
Authors: Lv Hualiang , Yao Yuxing , Yuan Mingyue , Chen Guanyu , Wang Yuchao , Rao Longjun , Li Shucong , Kara Ufuoma I. , Dupont Robert L. , Zhang Cheng , Chen Boyuan , Liu Bo , Zhou Xiaodi , Wu Renbing , Adera Solomon , Che Renchao , Zhang Xingcai , Wang Xiaoguang 
Published: February 2024

AbstractTwo-dimensional (2D) superlattices, formed by stacking sublattices of 2D materials, have emerged as a powerful platform for tailoring and enhancing material properties beyond their intrinsic characteristics. However, conventional synthesis methods are limited to pristine 2D material sublattices, posing a significant practical challenge when it comes to stacking chemically modified sublattices. Here we report a chemical synthesis method that overcomes this challenge by creating a unique 2D graphene superlattice, stacking graphene sublattices with monodisperse, nanometer-sized, square-shaped pores and strategically doped elements at the pore edges. The resulting graphene superlattice exhibits remarkable correlations between quantum phases at both the electron and phonon levels, leading to diverse functionalities, such as electromagnetic shielding, energy harvesting, optoelectronics, and thermoelectrics. Overall, our findings not only provide chemical design principles for synthesizing and understanding functional 2D superlattices but also expand their enhanced functionality and extensive application potential compared to their pristine counterparts.
In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography
Authors: Li Weimin , Li Angdi , Yu Bing , Zhang Xiaoxiao , Liu Xiaoyan , White Kate L. , Stevens Raymond C. , Baumeister Wolfgang , Sali Andrej , Jasnin Marion , Sun Liping 
Published: February 2024

AbstractActin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a “netlike” to a “blooming” architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion.