bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insight into the mechanism of CD34+ cell mobilisation impairment in multiple myeloma patients treated with anti‐CD38 therapy
Authors: Venglar Ondrej , Kapustova Veronika , Anilkumar Sithara Anjana , Zihala David , Muronova Ludmila , Sevcikova Tereza , Vrana Jan , Vdovin Alexander , Radocha Jakub , Krhovska Petra , Hrdinka Matous , Turjap Michal , Popkova Tereza , Chyra Zuzana , Broskevicova Lucie , Simicek Michal , Koristek Zdenek , Hajek Roman , Jelinek Tomas 
Published: October 2023

SummaryInduction therapy followed by CD34+ cell mobilisation and autologous transplantation represents standard of care for multiple myeloma (MM). However, the anti‐CD38 monoclonal antibodies daratumumab and isatuximab have been associated with mobilisation impairment, yet the mechanism remains unclear. In this study, we investigated the effect of three different regimens (dara‐VCd, isa‐KRd and VTd) on CD34+ cells using flow cytometry and transcriptomics. Decreased CD34+ cell peak concentration and yields, longer collection and delayed engraftment were reproduced after dara‐VCd/isa‐KRd versus VTd induction in 34 patients in total. Using flow cytometry, we detected major changes in the proportion of apheresis product and bone marrow CD34+ subsets in patients treated with regimens containing anti‐CD38 therapy; however, without any decrease in CD38high B‐lymphoid progenitors in both materials. RNA‐seq of mobilised CD34+ cells from 21 patients showed that adhesion genes are overexpressed in CD34+ cells after dara‐VCd/isa‐KRd and JCAD, NRP2, MDK, ITGA3 and CLEC3B were identified as potential target genes. Finally, direct in vitro effect of isatuximab in upregulating JCAD and CLEC3B was confirmed by quantitative PCR. These findings suggest that upregulated adhesion‐related interactions, rather than killing of CD34+ cells by effector mechanisms, could be leading causes of decreased mobilisation efficacy in MM patients treated with anti‐CD38 therapy.
Meloxicam treatment disrupts the regional structure of innate inflammation sites by targeting the pro‐inflammatory effects of prostanoids
Authors: Schäufele Tim J. , Kolbinger Anja , Friedel Joschua , Gurke Robert , Geisslinger Gerd , Weigert Andreas , Pierre Sandra , Scholich Klaus 
Published: October 2023

AbstractBackground and PurposeNon‐steroidal anti‐inflammatory drugs (NSAIDs) are the most widely prescribed drugs in the world due to their analgesic, antipyretic and anti‐inflammatory effects. However, NSAIDs inhibit prostanoid synthesis, interfering with their pro‐inflammatory and anti‐inflammatory functions and potentially prolonging acute inflammation.Experimental ApproachWe used high‐content immunohistochemistry to define the impact of meloxicam treatment on spatially separated pro‐inflammatory and anti‐inflammatory processes during innate inflammation in mice induced by zymosan. This allowed us to determine the effect of meloxicam treatment on the organization of pro‐inflammatory and anti‐inflammatory microenvironments, thereby identifying relevant changes in immune cell localization, recruitment and activation.Key ResultsMeloxicam treatment reduced zymosan‐induced thermal hypersensitivity at early time points but delayed its resolution. High‐content immunohistochemistry revealed that the pro‐inflammatory area was smaller after treatment, diminishing neutrophil recruitment, M1‐like macrophage polarization, and especially phagocytosis by neutrophils and macrophages. The polarization of macrophages towards the M2‐like anti‐inflammatory phenotype was unaffected, and the number of anti‐inflammatory eosinophils actually increased.Conclusion and ImplicationsHigh‐content immunohistochemistry was able to identify relevant meloxicam‐mediated effects on inflammatory processes based on alterations in the regional structure of inflammation sites. Meloxicam delayed the clearance of pathogens by inhibiting pro‐inflammatory processes, causing prolonged inflammation. Our data suggest that the prescription of NSAIDs as a treatment during an acute pathogen‐driven inflammation should be reconsidered in patients with compromised immune systems.
Finding navigation cues near fishways
Authors: Elings Jelger , Bruneel Stijn , Pauwels Ine S. , Schneider Matthias , Kopecki Ianina , Coeck Johan , Mawer Rachel , Goethals Peter L. M. 
Published: October 2023

ABSTRACTMany fish species depend on migration for various parts of their life cycle. Well‐known examples include diadromous fish such as salmon and eels that need both fresh water and salt water to complete their life cycle. Migration also occurs within species that depend only on fresh water. In recent decades, anthropogenic pressures on freshwater systems have increased greatly, and have resulted, among other effects, in drastic habitat fragmentation. Fishways have been developed to mitigate the resulting habitat fragmentation, but these are not always effective. To improve fishway efficiency, the variety of navigation cues used by fish must be better understood: fish use a multitude of sensory inputs ranging from flow variables to olfactory cues. The reaction of a fish is highly dependent on the intensity of the cue, the fish species involved, and individual traits. Recently developed monitoring technologies allow us to gain insights into different combinations of environmental and physiological conditions. By combining fish behavioural models with environmental models, interactions among these components can be investigated. Several methods can be used to analyse fish migration, with state‐space models, hidden Markov models, and individual‐based models potentially being the most relevant since they can use individual data and can tie them to explicit spatial locations within the considered system. The aim of this review is to analyse the navigational cues used by fish and the models that can be applied to gather knowledge on these processes. Such knowledge could greatly improve the design and operation of fishways for a wider range of fish species and conditions.
L'Enquête nationale sur les femmes et les filles autochtones disparues et assassinées au Canada: Explorer la relation entre l'existence de critiques externes et la prise de parole des témoins lors des audiences communautaires
Authors: Rousseau Audrey , Chartrand Louis 
Published: October 2023

AbstractFaced with the alarming rates of disappearances and murders of Indigenous women, girls and 2SLGBTQQIA+ people in Canada and in response to the demands of victims' families and Indigenous women's associations, the Canadian government set up the National Inquiry into Missing and Murdered Indigenous Women and Girls (2016–2019). Its mandate: to identify the systemic causes of violence and produce effective recommendations to remedy them. From its announcement and during the course of its work, the inquiry faced a great deal of criticism, particularly from families and Indigenous women's associations, undermining the trust of many in the commissioners and in the process. It was thus against the backdrop of those brewing tensions that many people affected by the violence came forward to tell their stories at community hearings held across the country. As we consider public testimony to be a vector of social agency for these witnesses, we ask how external critiques conveyed in the media sphere influenced these narrative spaces internal to the inquiry. Through the use of computer‐assisted text analysis (based in textometry) applied on a corpus of transcripts from the fifteen community hearings, we were able to identify the presence of certain criticisms, which occupied a relatively small space in the hearings. What's more, our explorations enabled us to reveal that witnesses bore a dual responsibility: to tell their story and to avoid downgrading the investigation in progress.
The sesquiterpene lactone mix: A review of past, present and future aspects
Authors: Paulsen Evy 
Published: October 2023

AbstractThe sesquiterpene lactones (SLs) are secondary plant metabolites, which are widespread in the Compositae/Asteraceae plant family. The first SLs were detected more than 100 years ago, and allergic contact dermatitis from Compositae has been reported since the beginning of the 1900s, but it was not until the late 1960s and early 1970s that a collaboration between dermatologists, chemists and botanists led to the detection of SLs as the main allergens of Compositae plants. In the 1980s, the SL mix, consisting of equimolar amounts of alantolactone, costunolide and dehydrocostus lactone, was developed as a screening agent for Compositae sensitisation. Today, after inclusion of SL mix in the baseline series, the mean prevalence of reactions in Europe is around 1%, and in North America 0.8%. In countries outside Europe and North America, the prevalence ranges between 0% and 10.7%. The detection rate of SL mix is lower than that of some plant extracts, and ideally, SL mix should be supplemented with a mix of SLs from locally prevalent allergenic plants. The prevalence of positive reactions to SL mix suggests continued baseline testing in most European countries, North America, New Zealand, Australia and probably some Chinese centres.
Colonoscopic‐assisted laparoscopic wedge resection versus segmental colon resection for benign colonic polyps: a comparative cost analysis
Authors: Hanevelt Julia , Leicher Laura W. , Moons Leon M. G. , Vleggaar Frank P. , Huisman Jelle F. , van Westreenen Henderik L. , de Vos tot Nederveen Cappel Wouter H. 
Published: October 2023

AbstractAimThe colonoscopic‐assisted laparoscopic wedge resection (CAL‐WR) is proven to be an effective and safe alternative to a segmental colon resection (SCR) for large or complex benign colonic polyps that are not eligible for endoscopic removal. This analysis aimed to evaluate the costs of CAL‐WR and compare them to the costs of an SCR.MethodA single‐centre 90‐day ‘in‐hospital’ comparative cost analysis was performed on patients undergoing CAL‐WR or SCR for complex benign polyps between 2016 and 2020. The CAL‐WR group consisted of 44 patients who participated in a prospective multicentre study (LIMERIC study). Inclusion criteria were (1) endoscopically unresectable benign polyps; (2) residual or recurrence after previous polypectomy; or (3) irradically resected low risk pT1 colon carcinoma. The comparison group, which was retrospectively identified, included 32 patients who underwent an elective SCR in the same period.ResultsColonoscopic‐assisted laparoscopic wedge resection was associated with significantly fewer complications (7% in the CAL‐WR group vs. 45% in the SCR group, P < 0.001), shorter operation time (50 min in the CAL‐WR group vs. 119 min in the SCR group, P < 0.001), shorter length of hospital stay (median length of stay 2 days in the CAL‐WR group vs. 4 days in the SCR group, P < 0.001) and less use of surgical resources (reduction in costs of 32% per patient), resulting in a cost savings of €2372 (£2099 GBP) per patient (P < 0.001).ConclusionGiven the clinical and financial benefits, CAL‐WR should be recommended for complex benign polyps that are not eligible for endoscopic resection before major surgery is considered.
Participatory Design for Cognitive Science: Examples From the Learning Sciences and Human−Computer Interaction
Authors: Chan Jenny Yun‐Chen , Nagashima Tomohiro , Closser Avery H. 
Published: October 2023

AbstractGiven the recent call to strengthen collaboration between researchers and relevant practitioners, we consider participatory design as a way to advance Cognitive Science. Building on examples from the Learning Sciences and Human−Computer Interaction, we (a) explore what, why, who, when, and where researchers can collaborate with community members in Cognitive Science research; (b) examine the ways in which participatory‐design research can benefit the field; and (c) share ideas to incorporate participatory design into existing basic and applied research programs. Through this article, we hope to spark deeper discussions on how cognitive scientists can collaborate with community members to benefit both research and practice.
SCN4B inhibits the progression of lung adenocarcinoma and is associated with better prognosis
Authors: Ma Minting , Guo Bin , Lu Hongwei , Hong Lei 
Published: October 2023

AbstractIntroductionLung adenocarcinoma (LUAD) is the major type of non‐small cell lung cancer with low a survival rate caused by metastasis. SCN4B encoding voltage‐gated sodium channel β subunit is regarded as a metastasis‐suppressor gene. We aim to explore how SCN4B influences the progression and prognosis of LUAD.MethodsThe gene expression profiles of 585 LUAD samples in TCGA and GSE31210, GSE116959, and GSE72094 datasets from the GEO database were downloaded for analysis. Differentially expressed genes were obtained through the “limma” package. The “clusterProfiler” package was used to conduct GSEA. Survival analysis was conducted via “survival” and “survminer” packages. Transcription factors regulating SCN4B expression were screened by correlation analysis and further predicted by FIMO. Infiltration of immune cells was analyzed by CIBERSORT. ESTIMATE algorithm was used to evaluate the immune‐related scores.ResultsSCN4B expressed higher in normal samples than in LUAD samples and higher in female samples than male samples. One hundred and twenty‐six pathways were significantly enriched between high and low SCN4B expression groups. Six transcription factors' expressions were positively related to SCN4B expression, and ChIP‐seq data from “Cistrome” verified that TAL1 and ERG might bind to the upstream sequence of SCN4B. SCN4B expression was significantly correlated with activated memory CD4 T cells, resting mast cells, and monocytes. TMB status, three scores based on ESTIMATE algorithm, and expression of three immune checkpoints showed significant differences between SCN4B high‐ and low‐expression groups. SCN4B could be considered as an independent prognostic signature of LUAD patients that higher expression represents a better prognosis.ConclusionSCN4B expresses higher in normal samples, and SCN4B is able to be an independent prognostic signature for LUAD patients. TAL1 and ERG may regulate the expression of SCN4B by binding its upstream sequences. Our research is valuable in improving the effectiveness of treatment in LUAD.
Isolation of Epstein‐Barr virus‐deoxyribonucleic acid in the lower respiratory tract for distinguishing critically ill patients from those with influenza‐associated pneumonia: A pilot study
Authors: Bai Yu , Guo Yinqun , Chen Xi , Yang Chunxia , Gu Li 
Published: October 2023

AbstractBackgroundWhen influenza A‐related pneumonia is complicated by bacteria, aspergillus, and other infections, the disease is aggravated, while there is no research on the role of Epstein–Barr virus (EBV) on patients with influenza A‐related pneumonia. This study aimed to evaluate the relationship between the isolation of EBV and influenza A‐related pneumonia.MethodsThis is a clinical study based on the baseline data of a retrospective cohort. A total of 113 cases of influenza A‐related pneumonia who underwent polymerase chain reaction test for isolation of EBV‐DNA in lower respiratory tract specimens during six influenza seasons from 2013–2014 to 2018–2019 were enrolled. According to the results of EBV‐DNA, patients were divided into EBV‐positive group and EBV‐negative group, and the role of EBV‐DNA on patients with influenza A‐related pneumonia was analyzed. Regression analysis was used to explore the potential risk factors for the development of moderate‐to‐severe acute respiratory distress syndrome (ARDS) in patients with influenza A‐related pneumonia during hospitalization.ResultsAmong 113 patients with influenza A‐related pneumonia, there were 53 patients with EBV‐positive and 60 patients with EBV‐negative. The EBV‐positive group had higher intensive care unit admission rate, hospital stay, invasive mechanical ventilation rate, extracorporeal membrane oxygenation rate, Sequential Organ Failure Assessment (SOFA) score, and moderate‐to‐severe ARDS rate. Patients were divided into severe group and mild group. Patients in severe group had lower lymphocyte count, platelet count, and albumin level, while the levels of aspartate aminotransferase, alanine transaminase, creatine kinase, lactic dehydrogenase, and total bilirubin were higher in severe group. The levels of D‐dimer, serum ferritin, C‐reactive protein, and procalcitonin were higher in severe group than those in the mild group. Multivariate logistic regression analysis revealed that the isolation of EBV (odds ratio = 2.713, 95% confidence interval: 1.094–6.729, P = 0.031) and lymphocyte count (odds ratio = 3.585, 95% confidence interval: 1.157–11.101, P = 0.027) were independent risk factors for moderate‐to‐severe ARDS in patients with influenza A‐related pneumonia.ConclusionThe isolation rate of EBV in the lower respiratory tract was 46.9%. The length of hospital stays, intensive care unit admission rate, invasive mechanical ventilation rate, extracorporeal membrane oxygenation rate, SOFA score, and the proportion of moderate‐to‐severe ARDS in the EBV‐positive group were higher than those in the EBV‐negative group, while there was no effect on the death during hospitalization. The isolation of EBV in the lower respiratory tract and low lymphocyte count are independent risk factors for the development of moderate‐to‐severe ARDS in patients with influenza A‐related pneumonia.
Pulmonary hemodynamics before and after pediatric heart transplantation
Authors: Biedermann Philipp , Sitte‐Koch Vanessa , Schweiger Martin , Meinold Anke , Quandt Daniel , Kretschmar Oliver , Balmer Christian , Knirsch Walter 
Published: October 2023

AbstractBackgroundPulmonary hypertension (PH) may limit the outcome of pediatric heart transplantation (pHTx). We evaluated pulmonary hemodynamics in children undergoing pHTx.MethodsCross‐sectional, single‐center, observational study analyzing pulmonary hemodynamics in children undergoing pHTx.ResultsTwenty‐three children (female 15) underwent pHTx at median (IQR) age of 3.9 (.9–8.2) years with a time interval between first clinical signs and pHTx of 1.1 (.4–3.2) years. Indications for pHTx included cardiomyopathy (CMP) (n = 17, 74%), congenital heart disease (CHD) (n = 5, 22%), and intracardiac tumor (n = 1, 4%). Before pHTx, pulmonary hemodynamics included elevated pulmonary artery pressure (PAP) 26 (18.5–30) mmHg, pulmonary capillary wedge pressure (PCWP) 19 (14–21) mmHg, left ventricular enddiastolic pressure (LVEDP) 17 (13–22) mmHg. Transpulmonary pressure gradient (TPG) was 6.5 (3.5–10) mmHg and pulmonary vascular resistance (Rp) 2.65 WU*m2 (1.87–3.19). After pHTx, at immediate evaluation 2 weeks after pHTx PAP decreased to 20.5 (17–24) mmHg, PCWP 14.5 (10.5–18) mmHg (p < .05), LVEDP 16 (12.5–18) mmHg, TPG 6.5 (4–12) mmHg, Rp 1.49 (1.08–2.74) WU*m2 resp.at last invasive follow up 4.0 (1.4–6) years after pHTx, to PAP 19.5 (17–21) mmHg (p < .05), PCWP 13 (10.5–14.5) mmHg (p < .05), LVEDP 13 (10.5–14) mmHg, TPG 7 (5–9.5) mmHg, Rp 1.58 (1.38–2.19) WU*m2 (p < .05). In CHD patients PAP increased (p < .05) after pHTx at immediate evaluation and decreased until last follow‐up (p < .05), while in CMP patients there was a continuous decline of mean PAP values immediately after HTx (p < .05).ConclusionsWhile PH before pHTx is frequent, after pHTx the normalization of PH starts immediately in CMP patients but is delayed in CHD patients.
Accessible digital images: AltText guidelines for authors and museum professionals
Authors: Cecilia Rafie , Moussouri Theano , Fraser John 
Published: October 2023

AbstractWith this issue, Curator: The Museum Journal has now implemented a policy requiring that all images contained in manuscripts published in our journal will require AltText descriptions in the main manuscript. The format for these descriptions should appear after figure location recommendations and figure captions in the main manuscript, indicated by capturing the beginning and end of the text with specific code for the typesetting team, and highlighting all text to be included in the HTML file in a red highlight as follows:<alt text>Statue of Lincoln</alt text>The guidelines for developing AltText can be found on the journal's Author Guidelines <https://onlinelibrary.wiley.com/page/journal/21516952/homepage/forauthors.html> section of the website. This editorial provides a short description of the work that led to this new policy, the principles of access and inclusion that support this new policy, and offers detailed guidelines for authors to support their development of manuscripts.
A Pentecostal theology of radical sharing: Sam‐Ae and ubuntu as critical hermeneutics of engaged love
Authors: Kgatle Mookgo Solomon , Kaunda Chammah J. 
Published: October 2023

AbstractThe article argues that although Pentecostal churches in Africa have the potential to challenge and transform the reality of inequalities in Africa, instead, they are reproducing and perpetuating these inequalities by creating an inequality gap among themselves, especially, between the pastors and their fellow congregants. A closer look at some of these churches reveals that some of them are propagating social, political, and economic inequalities demonstrated in the gap that exists between the pastors and their ordinary members. In response, we construct a Pentecostal theology of radical sharing to argue for a balanced distribution of wealth between the rich and the poor to deal with the challenges of inequalities. It demonstrates that indigenous idioms such as sam‐ae (Korea) and ubuntu (Africa) are critical hermeneutics from the margins for interpretative translation/contextualization of the Christian faith into a theology of radical sharing in the fight against inequalities within African Indigenous Pentecostalism.
Sustained metabolic benefits of ΔTRTX‐Ac1, a tarantula venom‐derived peptide, when administered together with exenatide in high‐fat fed mice
Authors: Coulter‐Parkhill Aimee , Tanday Neil , Cobice Diego , McLaughlin Christopher M. , McClean Stephen , Gault Victor A. , Irwin Nigel 
Published: October 2023

AbstractAimThe aim of the present study was to assess the long‐term therapeutic efficacy of a recently discovered 28 amino acid peptide, Δ‐theraphotoxin‐Ac1 (Δ‐TRTX‐Ac1), originally isolated from venom of the Aphonopelma chalcodes tarantula. Δ‐TRTX‐Ac has previously been shown to improve pancreatic beta‐cell function and suppress appetite.Materials and MethodsΔ‐TRTX‐Ac1 was administered twice daily in high‐fat fed (HFF) mice with streptozotocin (STZ)‐induced insulin deficiency, namely HFF/STZ mice, for 28 days both alone and in combination with the venom‐derived glucagon‐like peptide‐1 (GLP‐1) mimetic, exenatide.ResultsInitial pharmacokinetic profiling of ΔTRTX‐Ac1 revealed a plasma half‐life of 2 h in mice, with ΔTRTX‐Ac1 also evidenced in the pancreas 12 h post‐injection. Accordingly, HFF‐STZ mice received twice‐daily injections of Δ‐TRTX‐Ac1, exenatide or a combination of both peptides for 28 days. As anticipated, HFF/STZ mice presented with hyperglycaemia, impaired glucose tolerance, decreased plasma and pancreatic insulin and disturbed pancreatic islet morphology. Administration of ΔTRTX‐Ac1 reduced body weight, improved glucose tolerance and augmented pancreatic insulin content while decreasing glucagon content. Exenatide had similar benefits on body weight and pancreatic hormone content while also reducing circulating glucose. ΔTRTX‐Ac1 decreased energy expenditure on day 28 whereas exenatide had no impact. All treatment regimens restored pancreatic islet and beta‐cell area towards lean control levels, which was linked to significantly elevated beta‐cell proliferation rates. In terms of benefits of combined ΔTRTX‐Ac1 and exenatide treatment over individual agents, there was augmentation of glucose tolerance and ambulatory activity with combination therapy, and these mice presented with increased pancreatic glucagon.ConclusionThese data highlight the therapeutic promise of ΔTRTX‐Ac1 for diabetes, with suggestion that benefits could be enhanced through combined administration with exenatide.