bluuebunny/crossref_metadata_2025_split huggingface dataset:
United States insurance coverage of immediate lymphatic reconstruction
Authors: La‐Anyane Okensama , Alba Brandon E. , Harmon Kelly A. , To Jocelyn , Siotos Charalampos , Adepoju Jubril , Madrigrano Andrea , Alvarado Rosalinda , O'Donoghue Cristina , Perez Claudia B. , Kurlander David E. , Shenaq Deana S. , Kokosis George 
Published: November 2023

AbstractIntroductionImmediate Lymphatic Reconstruction (ILR) is a prophylactic microsurgical lymphovenous bypass technique developed to prevent breast cancer related lymphedema (BCRL). We investigated current coverage policies for ILR among the top insurance providers in the United States and compared it to our institutional experience with obtaining coverage for ILR.MethodsThe study analyzed the publicly available ILR coverage statements for American insurers with the largest market share and enrollment per state to assess coverage status. Institutional ILR coverage was retrospectively analyzed using deidentified claims data and categorizing denials based on payer reason codes.ResultsOf the 63 insurance companies queried, 42.9% did not have any publicly available policies regarding ILR coverage. Of the companies with a public policy, 75.0% deny coverage for ILR. In our institutional experience, $170,071.80 was charged for ILR and $166 118.99 (97.7%) was denied by insurance.ConclusionsOver half of America's major insurance providers currently deny coverage for ILR, which is consistent with our institutional experience. Randomized trials to evaluate the efficacy of ILR are underway and focus should be shifted towards sharing high level evidence to increase insurance coverage for BCRL prevention.
Neuroimaging Characteristics of Hearing Loss in the Mayo Clinic Study of Aging
Authors: Khandalavala Karl R. , Marinelli John P. , Lohse Christine M. , Przybelski Scott A. , Petersen Ronald C. , Vassilaki Maria , Vemuri Prashanthi , Carlson Matthew L. 
Published: November 2023

AbstractObjectiveTo investigate the association between standard pure tone and speech audiometry with neuroimaging characteristics reflective of aging and dementia in older adults.Study DesignProspective population‐based study.SettingSingle tertiary care referral center.MethodsParticipants from the Mayo Clinic Study of aging 60 years old or older with normal cognition or mild cognitive impairment, baseline neuroimaging, and a behavioral audiogram associated with neuroimaging were eligible for study. Imaging modalities included structural MRI (sMRI) and fluid‐attenuated inversion recovery MRI (FLAIR‐MRI; N = 605), diffusion tensor imaging MRI (DTI‐MRI; N = 444), and fluorodeoxyglucose‐positron emission tomography (FDG‐PET; N = 413). Multivariable logistic and linear regression models were used to evaluate associations with neuroimaging outcomes.ResultsMean (SD) pure tone average (PTA) was 33 (15) dB HL and mean (SD) word recognition score (WRS) was 91% (14). There were no significant associations between audiometric performance and cortical thinning assessed by sMRI. Each 10‐dB increase in PTA was associated with increased likelihood of abnormal white‐matter hyperintensity (WMH) from FLAIR‐MRI (odds ratio 1.26, P = .02). From DTI‐MRI, participants with <100% WRSs had significantly lower fractional anisotropy in the genu of the corpus callosum (parameter estimate [PE] −0.012, P = .008) compared to those with perfect WRSs. From FDG‐PET, each 10% decrease in WRSs was associated with decreased uptake in the anterior cingulate cortex (PE −0.013, P = .001).ConclusionPoorer audiometric performance was not significantly associated with cortical thinning but was associated with white matter damage relevant to cerebrovascular disease (increased abnormal WMH, decreased corpus callosum diffusion). These neuroimaging results suggest a pathophysiologic link between hearing loss and cerebrovascular disease.
Versican and versikine: The dynamism of the extracellular matrix
Authors: Watanabe Hideto 
Published: November 2023

AbstractVersican is a large chondroitin sulfate/dermatan sulfate proteoglycan in the extracellular matrix and one of the aggrecan/lectican family. Whereas versican is constitutively expressed and serves as a structural macromolecule in some tissues, it is transiently expressed at high levels when the extracellular matrix dynamically changes. There, versican plays an important role in forming the provisional matrix, which is replaced with the “authentic” extracellular matrix, that is, the matrix as it should be. ADAMTS‐1, 4, 5, 9, 15, and 20 cleave versican core protein and are therefore named versicanases. These proteinases have been believed to play a critical role in versican turnover. A cleaved N‐terminal fragment harbors biological functions, and it is termed “versikine.” This review discusses recent advances in the research on the in vivo function of versican and versikine generated by versicanases.
Formation of the Siberian Yamal gas emission crater via accumulation and explosive release of gas within permafrost
Authors: Schurmeier Lauren R. , Brouwer Gwendolyn E. , Fagents Sarah A. 
Published: November 2023

AbstractThe Arctic landscape has experienced many dramatic forms of change due to anthropogenic warming. Large crater‐like forms surrounded by fragmented blocks scattered outward to great distances with precursor mounds appeared in the continuous permafrost zone of the Yamal and Gydan peninsulas of Western Siberia. The morphologies of these craters, in addition to the abundant evidence of active and intense gas emissions across the region, indicate that they formed due the subsurface accumulation, pressurization, and explosive release of gasses trapped within the permafrost. Therefore, these craters were termed “gas emission craters” (GEC). However, some have suggested that in addition to gas accumulation, these features form in a manner similar to ice‐cored pingos and require pressurization due to the freezing of subsurface ice. This is despite the fact that not all GECs form in areas conducive to pingo‐like ice accumulation. Here, we test whether the pressurization and explosive release of methane gas alone can reproduce the observed morphology of the first‐discovered and most intensely studied GEC, Yamal crater. We use the available field and satellite data to constrain the initial dimension parameters of a model of the explosion process and consider several plausible configurations for the unknown interior cavity shape, the overlying permafrost cap thickness, and gas chamber volume. The explosion process is modeled in phases: gas migrates upward and accumulates in the subsurface until the gas pressure fractures the overlying cap, the expanding gas and cap blocks are initially accelerated outward together, and then the blocks are launched as projectiles. The sizes and locations of the ejected blocks found at Yamal crater are used to determine the initial gas pressures required to launch those projectiles to the observed locations. Finally, we estimate the amount of gas released in each of the multiple model runs testing different plausible internal cavity geometries. For the range of plausible block sizes and densities, and a subset of gas chamber volumes considered, we find that the gas pressure (0.6–2.6 MPa) required to launch the Yamal crater blocks to their observed distances was within the range of the sum of the ice/permafrost tensile strength and the lithostatic pressure (0.22–2.87 MPa). Thus, the observed blocks could have been launched by the energy required to fracture and displace the overlying permafrost cap. We show that the mechanism of formation of this GEC does not require pressure from the freezing of ice in the subsurface to crack and explode the overlying permafrost—gas pressure alone can produce these GECs. We expect that as our planet warms, the Siberian permafrost will continue to warm, weaken, and release gasses such as the greenhouse gas methane, and contribute to a permafrost carbon‐positive feedback cycle that would lead to the formation of even more explosive GECs.
Evaluating signaling bias for synthetic cannabinoid receptor agonists at the cannabinoid CB2 receptor
Authors: Patel Monica , Grimsey Natasha L. , Banister Samuel D. , Finlay David B. , Glass Michelle 
Published: November 2023

AbstractThe rapid structural evolution and emergence of novel synthetic cannabinoid receptor agonists (SCRAs) in the recreational market remains a key public health concern. Despite representing one of the largest classes of new psychoactive substances, pharmacological data on new SCRAs is limited, particularly at the cannabinoid CB2 receptor (CB2). Hence, the current study aimed to characterize the molecular pharmacology of a structurally diverse panel of SCRAs at CB2, including 4‐cyano MPP‐BUT7AICA, 4F‐MDMB‐BUTINACA, AMB‐FUBINACA, JWH‐018, MDMB‐4en‐PINACA, and XLR‐11. The activity of SCRAs was assessed in a battery of in vitro assays in CB2‐expressing HEK 293 cells: G protein activation (Gαi3 and GαoB), phosphorylation of ERK1/2, and β‐arrestin 1/2 translocation. The activity profiles of the ligands were further evaluated using the operational analysis to identify ligand bias. All SCRAs activated the CB2 signaling pathways in a concentration‐dependent manner, although with varying potencies and efficacies. Despite the detection of numerous instances of statistically significant bias, compound activities generally appeared only subtly distinct in comparison with the reference ligand, CP55940. In contrast, the phytocannabinoid THC exhibited an activity profile distinct from the SCRAs; most notably in the translocation of β‐arrestins. These findings demonstrate that CB2 is able to accommodate a structurally diverse array of SCRAs to generate canonical agonist activity. Further research is required to elucidate whether the activation of CB2 contributes to the toxicity of these compounds.
Resistance to the DMI fungicide mefentrifluconazole in Monilinia fructicola: risk assessment and resistance basis analysis
Authors: Li Guixiang , Zhang Ling , Wang Huakai , Li Xiuhuan , Cheng Fei , Miao Jianqiang , Peng Qin , Liu Xili 
Published: November 2023

AbstractBACKGROUNDBrown rot disease, caused by Monilinia fructicola, poses a significant challenge to peach production in China. The efficacy of mefentrifluconazole, a new triazole fungicide, in controlling brown rot in peaches has been remarkable. However, the resistance risk and mechanism associated with this fungicide remain unclear. This study was designed to assess the resistance risk of M. fructicola to mefentrifluconazole and reveal the potential resistance mechanism.RESULTSThe mean median effective concentration (EC50) of 101 M. fructicola isolates to mefentrifluconazole was 0.003 μg mL−1, and the sensitivity exhibited a unimodal distribution. Seven mefentrifluconazole‐resistant mutants were generated from three parental isolates in the laboratory through fungicide adaption. The biological characteristics of the resistant mutants revealed that three of them exhibited enhanced survival fitness compared to the parental isolates, whereas the remaining four mutants displayed reduced survival fitness. Mefentrifluconazole showed strong positive cross‐resistance with fenbuconazole, whereas no cross‐resistance was observed with pyrimethanil, procymidone or pydiflumetofen. No overexpression of MfCYP51 gene was detected in the resistant mutants. Multiple sequence alignment revealed that three resistant mutants (MXSB2‐2, Mf12‐1 and Mf12‐2) had a point mutation (G461S) in MfCYP51 protein. Molecular docking techniques confirmed the contribution of this point mutation to mefentrifluconazole resistance.CONCLUSIONThe risk of M. fructicola developing resistance to mefentrifluconazole is relatively low‐to‐medium and point mutation G461S in MfCYP51 could confer mefentrifluconazole resistance in M. fructicola. This study provided essential data for monitoring the emergence of resistance and developing resistance management strategies for mefentrifluconazole. © 2023 Society of Chemical Industry.
Moment‐based outcrossing rate method for time‐dependent reliability assessment including finite element analysis
Authors: Li Xiang‐Wei , Zhang Xuan‐Yi , Zhao Yan‐Gang 
Published: November 2023

AbstractIn practice, structural limit state function (LSF) is often combined with finite element (FE) models, and the corresponding time‐dependent reliability assessment (TRA) inevitably involves dealing with computational inefficiency. In this paper, a general moment‐based outcrossing rate (GMO) method is proposed to conduct TRA efficiently, with no limitation in the form of LSF. There are two innovations of GMO method: firstly, an explicit moment‐based outcrossing rate is developed; secondly, a separate algorithm is proposed that facilitates the separation of FE analysis from TRA cycle. The proposed method minimizes the computational cost associated with FE analysis and enables TRA cycle to be conducted more efficiently with the assistance of an explicit moment‐based outcrossing rate and explicit moment‐based outcrossing rate. Three numerical examples are investigated to examine the accuracy and efficiency of GMO method for TRA cycle. GMO method is found from the results that it can efficient and accurate to conduct TRA. Such improvements in computational efficiency are particularly valuable for complex structures where FE analysis is computationally expensive and time‐consuming.
Toward atomistic models of intact severe acute respiratory syndrome coronavirus 2 via Martini coarse‐grained molecular dynamics simulations
Authors: Wang Dali , Li Jiaxuan , Wang Lei , Cao Yipeng , Kang Bo , Meng Xiangfei , Li Sai , Song Chen 
Published: November 2023

AbstractThe causative pathogen of coronavirus disease 2019 (COVID‐19), severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), is an enveloped virus assembled by a lipid envelope and multiple structural proteins. In this study, by integrating experimental data, structural modeling, as well as coarse‐grained and all‐atom molecular dynamics simulations, we constructed multiscale models of SARS‐CoV‐2. Our 500‐ns coarse‐grained simulation of the intact virion allowed us to investigate the dynamic behavior of the membrane‐embedded proteins and the surrounding lipid molecules in situ. Our results indicated that the membrane‐embedded proteins are highly dynamic, and certain types of lipids exhibit various binding preferences to specific sites of the membrane‐embedded proteins. The equilibrated virion model was transformed into atomic resolution, which provided a 3D structure for scientific demonstration and can serve as a framework for future exascale all‐atom molecular dynamics (MD) simulations. A short all‐atom molecular dynamics simulation of 255 ps was conducted as a preliminary test for large‐scale simulations of this complex system.
Doping control approach: Identification of equine in vitro metabolites of voxelotor (GBT440), a hemoglobin S polymerization inhibitor
Authors: Subhahar Michael Benedict , Karakka Kal Abdul Khader , Philip Moses , Muhammed Ajeebsanu Meleparappil , Karatt Tajudheen K. , Perwad Zubair 
Published: November 2023

RationaleSickle cell disease, a debilitating genetic disorder affecting numerous newborns globally, has historically received limited attention in pharmaceutical research. However, recent years have witnessed a notable shift, with the Food and Drug Administration approving three innovative disease‐modifying medications. Voxelotor, also known as GBT440, is a promising compound that effectively prevents sickling, providing a safe approach to alleviate chronic hemolytic anemia in sickle cell disease. It is a novel, orally bioavailable small molecule that inhibits hemoglobin S polymerization by enhancing oxygen affinity to hemoglobin. The investigation demonstrated that voxelotor led to an unintended elevation of hemoglobin levels in healthy individuals by increasing serum erythropoietin levels.MethodsVoxelotor and its metabolites in an in vitro setting utilizing equine liver microsomes were discussed. Plausible structures of the identified metabolites were inferred through the application of liquid chromatography in conjunction with high‐resolution mass spectrometry.ResultsUnder the experimental conditions, a total of 31 metabolites were detected, including 16 phase I metabolites, two phase II metabolites, and 13 conjugates of phase I metabolites. The principal phase I metabolites were generated through processes such as hydroxylation, reduction, and dissociation. The presence of glucuronide and sulfate conjugates of the parent drug were also observed, along with hydroxylated, reduced, and dissociated analogs.ConclusionsThe data acquired will accelerate the identification of voxelotor and related compounds, aiding in the detection of their illicit use in competitive sports. It is crucial to emphasize that the metabolites detailed in this manuscript were identified through in vitro experiments and their detection in an in vivo study may not be guaranteed.
Long‐term outcome following successful endoscopic closure of tracheo‐oesophageal fistulas with two cardiac amplatzer septal occluders in a patient with oesophageal cancer
Authors: Schreder Theresa , Borbély Yves , Christen Stefan , Wiest Reiner , Räber Lorenz , Günther Gunar 
Published: November 2023

AbstractAcquired tracheo‐oesophageal fistulas (TEFs) are rare and challenging complications in the course of oesophageal cancer. While surgery is the only curative treatment option for TEFs many patients are not eligible for surgery. Endoscopic treatment approaches such as tracheal‐ and/or oesophageal‐ stenting are available, but associated with complications like the development of new fistulas and mucus retention. Off‐ label‐use of cardiac amplatzer occluder devices to close TEFs has been reported in few case‐reports with inconsistent short‐term outcomes. We report a case of successful closure of two adjacent TEFs with two partially overlapping cardiac amplatzer occluder devices. The insertion of a 12 mm and a 9 mm device was successful and without complications. The patient tolerated the cardiac amplatzer‐devices well and could resume oral food uptake after 2 months. Two years after closure, the patient remained free of symptoms suggesting complete sealing of the fistulas.
Improving visual acuity with nivolumab plus ipilimumab plus two cycles of chemotherapy following a diagnosis of lung adenocarcinoma with choroidal metastasis: A case report and literature review
Authors: Matsuyama Takahiro , Oniwa Masashi , Tsuruzono Kentaro , Yasuda Shunsuke , Yone Mikiko , Tomioka Yuya , Uchida Akifumi , Mitsuyama Hideo , Kubota Shingo , Suetsugu Takayuki , Mizuno Keiko , Inoue Hiromasa 
Published: November 2023

AbstractA 75‐year‐old woman presented at our hospital with bilateral visual impairment. Ophthalmological examination revealed multiple choroidal tumours. Chest computed tomography revealed a tumour shadow in the right lower lobe and multiple lymph node metastases in the mediastinum and pulmonary hilum. Following a detailed examination, the patient was diagnosed with primary lung adenocarcinoma (cT1cN3M1c Stage IVB) with choroid metastases. The tumour proportion score of programmed death ligand 1 (PD‐L1) was 1% and EGFR exon 20 insertion mutations were also detected. The patient was administered combination chemotherapy with nivolumab and ipilimumab. Primary lung and metastatic tumours, including the choroid, were reduced, and visual disturbances improved completely. Herein, we describe a rare case in which a combination of chemotherapy with nivolumab and ipilimumab significantly reduced vision loss due to choroidal metastasis.
Minute pulmonary meningothelial‐like nodule that was difficult to differentiate from lung adenocarcinoma due to specific computed tomography findings changes: A case report
Authors: Ohashi Takuya , Kawago Mitsumasa , Iguchi Hideto , Sakaguchi Masahiro , Sagan Kanako , Kojima Fumiyoshi , Nishimura Yoshiharu 
Published: November 2023

AbstractMinute pulmonary meningothelial‐like nodules (MPMNs) are benign lesions characterized by the appearance of ground‐glass nodules (GGN) on computed tomography (CT). In the present case, an MPMN gradually developed into a substantial component during chest CT follow‐up, and the GGN gradually transformed into a part‐solid nodule. The imaging course described in this case is quite unique. Such CT images are characteristic of malignant tumours, especially, highly differentiated adenocarcinomas, which are difficult to differentiate preoperatively. Therefore, it is important to report this case.
Elementary preservice teachers' responsiveness while eliciting students' initial arguments and encouraging critique in online simulated argumentation discussions
Authors: Lottero‐Perdue Pamela S. , Masters Heidi L. , Mikeska Jamie N. , Thompson Meredith , Park Rogers Meredith , Cross Francis Dionne 
Published: November 2023

AbstractEngaging children in argumentation‐focused discussions is essential to helping them collaboratively make sense of scientific phenomena. To support this effort, teachers must listen and be responsive to students' ideas to move the discussion forward with the goal of reaching consensus. Given the complexity of this ambitious science teaching practice, in lieu of traditional field experiences, online simulated teaching experiences provide opportunities for preservice teachers to practice implementing these strategies in a low‐risk, high‐support environment. Limited research has explored elementary preservice teachers' responsiveness while navigating an argumentation‐focused discussion, particularly in an online simulated teaching experience. The purpose of this study was to examine preservice teachers' responsiveness to students' ideas while eliciting students' initial constructed arguments and encouraging argument critique in two online simulated teaching experiences. Findings showed that preservice teachers' responsiveness to students' ideas was high in both online simulated teaching experiences when asking students to share evidence as well as engage in critique. However, their responsiveness varied when prompting for reasoning and was often low when eliciting students' claims. These findings provide empirical evidence that such online simulated teaching experiences can be used as productive spaces for PSTs to practice being responsive to students' ideas during argumentation‐focused discussions.