bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detailed assessment of chromosome 22 aberrations in sporadic pheochromocytoma using array‐CGH
Authors: Jarbo Caroline , Buckley Patrick G. , Piotrowski Arkadiusz , Mantripragada Kiran K. , Benetkiewicz Magdalena , de Ståhl Teresita Diaz , Langford Cordelia F. , Gregory Simon G. , Dralle Henning , Gimm Oliver , Bäckdahl Martin , Geli Janos , Larsson Catharina , Westin Gunnar , Åkerström Göran , Dumanski Jan P. 
Published: September 2005

AbstractPheochromocytoma is a predominantly sporadic neuroendocrine tumor derived from the adrenal medulla. Previous low resolution LOH and metaphase‐CGH studies reported the loss of chromosomes 1p, 3q, 17p and 22q at various frequencies. However, the molecular mechanism(s) behind development of sporadic pheochromocytoma remains largely unknown. We have applied high‐resolution tiling‐path microarray‐CGH with the primary aim to characterize copy number imbalances affecting chromosome 22 in 66 sporadic pheochromocytomas. We detected copy number alterations on 22q at a frequency of 44%. The predominant finding was monosomy 22 (30%), followed by terminal deletions in 8 samples (12%) and a single interstitial deletion. We further applied a chromosome 1 tiling‐path array in 7 tumors with terminal deletions of 22q and found deletions of 1p in all cases. Our overall results suggest that at least 2 distinct regions on both 22q and 1p are important in the tumorigenesis of sporadic pheochromocytoma. A large proportion of pheochromocytomas also displayed indications of cellular heterogeneity. Our study is to our knowledge the first array‐CGH study of sporadic pheochromocytoma. Future analysis of this tumor type should preferably be performed in the context of the entire human genome using genome‐wide array‐CGH, which is a superior methodological approach. Supplemental material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020‐7136/suppmat/index.html. © 2005 Wiley‐Liss, Inc.
Evaluation of a new fiber‐reinforced resin composite
Authors: Suzuki S. , Saimi Y. , Ono T. 
Published: September 2005

AbstractEfficacy of the usage of an experimental fiber‐reinforced composite (FRC) on mechanical properties of an indirect composite was investigated by means of three‐point bending and Charpy impact tests. Bond strength between the FRC and the indirect composite was also evaluated by tensile testing. The FRC consisted of a matrix resin with 25% silanized milled glass fiber (11‐μm diameter, 150‐μm length) and 5% colloidal silica. The values of strain of proportional limit, total strain, and fracture energy of the FRC during the bending test (1.2%, 10.4%, and 41.6 × 10−3 J) were significantly higher than those of the indirect composite (0.1%, 2.5%, and 11.9 × 10−3 J). The impact strengths of the 1‐mm specimens with FRC ranged from 15.2 to 15.9 kJ/m2, and were significantly higher than that of the control (3.1 kJ/m2). The 2‐mm specimens showed significant difference from the control when the FRC thickness was equal or greater than 0.5 mm. The bond strength after the thermocycling was 15.2 MPa, and all of the specimens exhibited cohesive fracture inside the indirect composite. Based upon the results, it was concluded that the FRC tested in this study improved toughness and impact resistance of the indirect composite. The interfacial bonding between the FRC and the indirect composite was strong enough to prevent delamination. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Effect of silver addition on the properties of nickel–titanium alloys for dental application
Authors: Oh Keun‐Taek , Joo Uk‐Hyon , Park Gee‐Ho , Hwang Chung‐Ju , Kim Kyoung‐Nam 
Published: September 2005

AbstractEquiatomic and near‐equiatomic nickel–titanium alloys exhibit a shape‐memory effect and superelasticity. However, the properties of such alloys are extremely sensitive to the precise nickel–titanium ratio and the addition of alloying elements. High corrosion resistance is necessary for biomedical applications, especially orthodontic. The purpose of this study was to investigate the effect of silver addition to nickel–titanium alloys for dental and medical application. Arc melting, homogenization, hot rolling, and solution heat treatment were performed to prepare the nickel–titanium–silver (NiTi‐Ag) specimens. The properties of the ternary NiTi–Ag alloys such as phase‐transformation temperature, microstructure, microhardness, corrosion resistance, and cytotoxicity were investigated. The NiTi‐Ag alloys showed low silver recovery rate for the cast alloy, due to silver's low evaporation temperature, and low silver solubility in nickel–titanium. Silver addition to nickel–titanium increased the transition temperature range to 100°C and stabilized the martensitic phase (monoclinic structure) at room temperature, because the martensitic transformation starting temperature (Ms) was above room temperature. Martensitic and austenitic phases existed in X‐ray diffraction patterns of solution‐annealed NiTi‐Ag alloys. The silver addition was considered to improve the corrosion resistance and form a stable passive film. Significantly, the mechanical properties of the silver‐added alloys were dependent upon the amount of alloying addition. There was no toxicity in the NiTi‐Ag alloys, as the response index showed none or mild levels. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Influence of polymerization method, curing process, and length of time of storage in water on the residual methyl methacrylate content in dental acrylic resins
Authors: Bayraktar Gulsen , Guvener Bora , Bural Canan , Uresin Yagiz 
Published: September 2005

AbstractThis study compared the influence of different polymerization methods (heat, auto‐, and microwave energy), different curing processes (in the case of heat‐ and autopolymerized specimens), and length of storage of the polymerized specimens in distilled water at 37°C on the residual methyl methacrylate (MMA) content in dental acrylic resin specimens. Residual MMA of 120 resin specimens were measured using high‐performance liquid chromatography. For the heat‐polymerized resins, the lowest residual MMA content was obtained when they were given a long‐term terminal boil and then stored in the distilled water for at least 1 day. For the autopolymerized resins, the lowest residual MMA content was obtained when they were additionally cured in water at 60°C and then stored in the distilled water at least 1 day. For the microwave‐polymerized resins, the lowest residual MMA content was obtained when they were stored in the distilled water at least 1 month. The lowest overall residual MMA content was obtained from heat‐polymerized specimens that were given a long‐term terminal boil cure and then stored in the distilled water at least 1 day. Different polymerization methods and curing processes have different effects on residual MMA content. It is thus shown that storing a dental acrylic resin specimen in distilled water at 37°C is a simple but effective method of reducing its residual MMA content. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Influence of the remelting process on the fatigue behavior of electron beam irradiated UHMWPE
Authors: Puértolas J. A. , Medel F. J. , Cegoñino J. , Gomez‐Barrena E. , Ríos R. 
Published: September 2005

AbstractElectron beam irradiation at doses below 150 kGy is a widely used technique to obtain highly crosslinked ultra‐high‐molecular‐weight polyethylene (UHMWPE). Its current use in total joint replacement components may improve wear resistance and decrease UHMWPE particle debris. However, currently used post‐irradiation thermal treatments, which aim to decrease the free radicals within the material, introduce microstructural changes that affect UHMWPE mechanical properties, particularly the fatigue strength. This influence may be crucial in total knee replacements, where fatigue‐related damage limits the lifespan of the prosthesis. Therefore, more studies are required to understand UHMWPE fatigue after current crosslinking protocols. This study was planned to evaluate the influence of UHMWPE remelting after irradiation on the material fatigue resistance. The remelting was achieved at 150°C for 2 h on UHMWPE previously irradiated at 50, 100, and 150 kGy. Fatigue evaluation included short‐term tests under cyclic tensile stress with zero load ratio, R = 0, and 1 Hz. In addition, stress‐life testing was performed using 12% yield as the criterion for failure. Near‐threshold fatigue crack propagation experiments were also performed at a frequency of 5 Hz, and crack length was measured in nonthermally treated and remelted irradiated UHMWPE. Crystallinity percentage was calculated from DSC measurements. The results pointed out that irradiation positively contributed to total life analysis, but the further remelting process decreased the flaw initiation resistance. On the other hand, both processes negatively affected the fatigue resistance of notched components. From a clinical point of view, the results suggest that the material fatigue behavior should be carefully studied in new UHMWPE to avoid changes related to material processing. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Treating couples recovering from infidelity: An integrative approach
Authors: Gordon Kristina Coop , Baucom Donald H. , Snyder Douglas K. 
Published: September 2005

AbstractInfidelity is one of the most difficult problems to address in couple therapy, most likely because it involves a traumatic relationship event that alters the ways in which couples process information about each other and established behavioral patterns. We present a three‐stage treatment designed to address the cognitive, behavioral, and emotional sequelae of affairs that integrates cognitive‐behavioral and insight‐oriented strategies with the literatures on traumatic response and forgiveness. A case study with pretreatment, posttreatment, and 6‐month follow‐up data is presented to illustrate the treatment methods. © 2005 Wiley Periodicals, Inc. J Clin Psychol/In Session 61: 1393–1405, 2005.
Local delivery of magnetic resonance (MR) contrast agent in kidney using thermosensitive liposomes and MR imaging‐guided local hyperthermia: A feasibility study in vivo
Authors: Salomir Rares , Palussière Jean , Fossheim Sigrid L. , Rogstad Astri , Wiggen Unni N. , Grenier Nicolas , Moonen Chrit T.W. 
Published: September 2005

AbstractPurposeTo investigate the feasibility of local delivery of a magnetic resonance (MR) contrast agent in vivo using paramagnetic thermosensitive liposomes and infrared (IR) laser‐induced local hyperthermia under real‐time MR thermometry on rabbit kidney.Materials and MethodsRespiratory gated, radio frequency (RF)‐spoiled gradient‐echo sequences were used for precise MR temperature mapping (SD = 1°C). In vivo heating experiments confirmed local release of MR contrast agent from liposomes.ResultsT1 decreased from 800 msec to about 500 msec, as measured after tissue cooling, in those locations where the renal parenchyma was heated above the phase transition temperature of the liposome membrane.ConclusionThe release of MR contrast agent has been demonstrated in rabbit kidney in vivo. This may be used as a reporter for simultaneous release of therapeutic agents. J. Magn. Reson. Imaging 2005. © 2005 Wiley‐Liss, Inc.
Pisa syndrome without neuroleptic exposure in a patient with Parkinson's disease: Case report
Authors: Gambarin Mattia , Antonini Angelo , Moretto Giuseppe , Bovi Paolo , Romito Silvia , Fiaschi Antonio , Tinazzi Michele 
Published: September 2005

AbstractWe report on a patient affected by Parkinson's disease who developed over a period of a few weeks a tonic deviation of her head, neck, and trunk fitting the typical description of Pisa syndrome (PS). This patient was under stable levodopa and pramipexole treatment and had never been exposed to any psychotropic or antiemetic drugs before or at the time she developed the postural abnormality. Because dopamine transporter imaging revealed bilateral and symmetrical reduction of striatal uptake, we suggest that PS is not primarily related to side differences in dopaminergic denervation or drug exposure. © 2005 Movement Disorder Society
Integrated data acquisition and processing to determine metabolite contents, relaxation times, and macromolecule baseline in single examinations of individual subjects
Authors: Kreis Roland , Slotboom Johannes , Hofmann Lucie , Boesch Chris 
Published: September 2005

AbstractAbsolute quantitation of clinical 1H‐MR spectra is virtually always incomplete for single subjects because the separate determination of spectrum, baseline, and transverse and longitudinal relaxation times in single subjects is prohibitively long. Integrated Processing and Acquisition of Data (IPAD) based on a combined 2‐dimensional experimental and fitting strategy is suggested to substantially improve the information content from a given measurement time. A series of localized saturation‐recovery spectra was recorded and combined with 2‐dimensional prior‐knowledge fitting to simultaneously determine metabolite T1 (from analysis of the saturation‐recovery time course), metabolite T2 (from lineshape analysis based on metabolite and water peak shapes), macromolecular baseline (based on T1 differences and analysis of the saturation‐recovery time course), and metabolite concentrations (using prior knowledge fitting and conventional procedures of absolute standardization). The procedure was tested on metabolite solutions and applied in 25 subjects (15–78 years old). Metabolite content was comparable to previously found values. Interindividual variation was larger than intraindividual variation in repeated spectra for metabolite content as well as for some relaxation times. Relaxation times were different for various metabolite groups. Parts of the interindividual variation could be explained by significant age dependence of relaxation times. Magn Reson Med, 2005. © 2005 Wiley‐Liss, Inc.
Instant MR labeling of stem cells using magnetoelectroporation
Authors: Walczak P. , Kedziorek D. A. , Gilad A. A. , Lin S. , Bulte J. W. M. 
Published: September 2005

AbstractFor cellular MR imaging, conventional approaches to intracellular magnetic labeling of nonphagocytic cells rely on the use of secondary compounds such as transfection agents and prolonged incubation of cells. Magnetoelectroporation (MEP) was investigated as an alternative method to achieve instant (<1 s) endosomal labeling with the FDA‐approved formulation Feridex, without the need for adjunct agents or initiating cell cultures. While MEP was harmful at higher voltages or pulse durations, the procedure could be properly calibrated using a pulse of 130 V and 17 ms. Labeling was demonstrated for stem cells from mice, rats, and humans; the uptake of iron was in the picogram range and comparable to values obtained using transfection agents. MEP‐labeled stem cells exhibited an unaltered viability, proliferation, and mitochondrial metabolic rate. Labeled mesenchymal stem cells (MSCs) and neural stem cells (NSCs) differentiated into adipogenic, osteogenic, and neural lineages in an identical fashion as unlabeled cells, while containing Feridex particles as demonstrated by double immunofluorescent staining. MEP‐labeled NSCs proliferated normally following intrastriatal transplantation and could be readily detected by MR imaging in vivo. As MEP circumvents the use of secondary agents, obviating the need for clinical approval, MEP labeling may be ideally suitable for bedside implementation. Magn Reson Med, 2005. © 2005 Wiley‐Liss, Inc.
HPLC coupled on‐line to ESI‐MS and a DPPH‐based assay for the rapid identification of anti‐oxidants in Butea superba
Authors: Nuengchamnong N. , de Jong C. F. , Bruyneel B. , Niessen W. M. A. , Irth H. , Ingkaninan K. 
Published: September 2005

AbstractA reversed‐phase HPLC coupled on‐line to a radical scavenging detection system and MS/MS was developed in order to combine separation, activity determination and structural identification of anti‐oxidants in complex mixtures in one run. The sample was separated by HPLC and the eluate split into two flows. The major portion was fed into an electrospray ionisation MS/MS system, while the minor part was mixed with a free radical, 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH), and the reaction determined spectrophotometrically. The negative peaks, which indicated the presence of anti‐oxidant activity, were monitored by measuring the decrease in absorbance at 517 nm. The developed method was successfully applied to the identification of anti‐oxidant compounds in a fraction, obtained by solid‐phase extraction, of an extract of a Thai medicinal plant, Butea superba Roxb. The anti‐oxidant compounds were separated and identified as procyanidin B2, (−)‐epicatechin and procyanidin B5. Copyright © 2005 John Wiley & Sons, Ltd.
Novel lysine‐peptoid hybrids with antibacterial properties
Authors: Ryge Trine S. , Hansen Paul R. 
Published: September 2005

AbstractHere we report the design, synthesis and antibacterial activity of 20 lysine‐peptoid hybrids. The hybrids are based on the peptoid lead structure [N‐(1‐naphthalenemethyl)glycyl]‐[N‐(4‐methylbenzyl)glycyl]‐[N‐(1‐naphthalenemethyl)glycyl]‐N‐(butyl)glycin amide (1) and contain between one and six lysine residues each. The compounds were tested for antibacterial activity against S. aureus ATCC 25923 and E. coli ATCC 25922. Furthermore, the hemolytic activity toward human erythrocytes was assessed. Several compounds with potent antibacterial activity and low hemolytic activity were identified. Copyright © 2005 European Peptide Society and John Wiley & Sons, Ltd.
Altered dendritic arborization of amygdala neurons in young adult rats orally intubated with Clitorea ternatea aqueous root extract
Authors: Rai Kiranmai S. , Murthy K. Dilip , Rao Muddanna S. , Karanth K. Sudhakar 
Published: September 2005

AbstractYoung adult (60 day old) Wistar rats of either sex were orally intubated with 50 mg/kg body weight and 100 mg/kg body weight of aqueous root extract of Clitoria ternatea (CTR) for 30 days, along with age‐matched saline controls. These rats were then subjected to passive avoidance tests and the results from these studies showed a significant increase in passive avoidance learning and retention. Subsequent to the passive avoidance tests, these rats were killed by decapitation. The amygdala was processed for Golgi staining and the stained neurons were traced using a camera lucida and analysed. The results showed a significant increase in dendritic intersections, branching points and dendritic processes arising from the soma of amygdaloid neurons in CTR treated rats especially in the 100 mg/kg group of rats, compared with age‐matched saline controls. This improved dendritic arborization of amygdaloid neurons correlates with the increased passive avoidance learning and memory in the CTR treated rats as reported earlier. The results suggest that Clitoria ternatea aqueous root extract enhances memory by increasing the functional growth of neurons of the amygdala. Copyright © 2005 John Wiley & Sons, Ltd.
Antimicrobial activity of antibiotics in combination with natural flavonoids against clinical extended‐spectrum β‐lactamase (ESBL)‐producing Klebsiella pneumoniae
Authors: Lin Rong‐Dih , Chin Yi‐Ping , Lee Mei‐Hsien 
Published: September 2005

AbstractExtended‐spectrum β‐lactamases (ESBLs) are plasmid‐mediated class A enzymes commonly found in the family Enterobacteriaceae, mainly in Klebsiella pneumoniae. Flavonoids have also been reported to possess antimicrobial activity. In this study, the in vitro activities of 18 antibiotics and 12 flavonoids against 20 ESBL‐producing K. pneumoniae isolates were evaluated. All of these isolates were susceptible to imipenem and cefmetazole, but were resistant to ampicillin, ampicillin/sulbactam, aztreonam, cefazolin, cefoperazone, cefotaxime, ceftazidime, ceftriaxone, cefuroxime, piperacillin and ticarcillin. Susceptibilities to amikacin, amoxicillin/clavulanate, cefoxitin, ciprofloxacin and gentamicin were variable. Myricetin, a flavonol, inhibited ESBL‐producing K. pneumoniae isolates at a high minimum inhibitory concentration (MIC) (MIC90 value 256 mg/mL), but exhibited significant synergic activity against ESBL‐producing K. pneumoniae in separate combination with amoxicillin/clavulanate, ampicillin/sulbactam and cefoxitin. Because of the low‐toxic nature of flavonoids, the combination of antibiotics and flavonoids is a potential new strategy for developing therapies for infections caused by ESBL‐producing bacteria in the future. Copyright © 2005 John Wiley & Sons, Ltd.
Identification of toxicants from marine sediment using effect‐directed analysis
Authors: Grote Matthias , Brack Werner , Altenburger Rolf 
Published: September 2005

AbstractEffect‐directed analysis (EDA) has been reported to be a powerful tool for the identification of the responsible toxicants in contaminated, hazardous environmental samples. The aim of this study was to investigate whether it also is possible to use currently available EDA methodology to identify potentially relevant toxicants in samples that do not pose obvious problems. For this purpose, compounds extracted from a marine sediment sample from the west coast of Sweden were separated into distinct fractions, using two preparative chromatographic techniques. One algal bioassay using Scenedesmus vacuolatus and two bacterial bioassays using Vibrio fischeri were applied as detectors of toxicity, representing acute and chronic end points. Chronic algal toxicity was a powerful tool for discriminating between toxic and nontoxic fractions, whereas acute and chronic bacterial toxicity failed to identify toxic fractions. Eight compounds were identified as potentially relevant toxicants by chemical analysis of toxic fractions: anthracene, fluoranthene, pyrene, benzo[a]anthracene, benzo[b]fluoranthene, benzo[a]pyrene, benzo[k]fluoranthene, and indeno[1,2,3‐cd]pyrene. © 2005 Wiley Periodicals, Inc. Environ Toxicol 20: 475–486, 2005.
Pre‐operative evaluation of patients with low rectal carcinoma
Authors: Durdey Paul , Williams Norman S. 
Published: September 2005

AbstractAdvances in surgical technique and adjuvant therapy have broadened the spectrum of treatment available for patients with low rectal cancer. Combination of careful pre‐operative clinical examination with newer, more sophisticated techniques, particularly in the field of imaging, can stage the extent of disease more accurately then previously. Adoption of an extended pre‐operative evaluation allows a more rational approach to treatment in the individual, and standardizes criteria for entry into, and interpretation of, future clinical trials.
Technique of per anal excision for carcinoma of the low rectum
Authors: Nivatvongs Santhat , Wolff Bruce G. 
Published: September 2005

AbstractPer anal excision is suitable for rectal carcinoma that is <3 cm and has not invaded into the muscular layer of the rectum. The upper margin of the lesion should not be >7 cm from the anal verge. The entire lesion is excised in its full thickness with a 1.5 cm to 2 cm normal margin, using electrocautery. It is important that the specimen is carefully examined by the pathologist for completeness of the excision and grade of the carcinoma. Abdominoperineal resection should be considered in good risk patients if adequate margins cannot be achieved and if the carcinoma is a high pathologic grade.