bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of nutritional habits, body mass index and intestinal microbiota in mastocytosis on clinical symptoms using conventional culture and next generation sequencing
Authors: Harcęko‐Zielińska Ewelina , Niedoszytko Marek , Górska Aleksandra , Małgorzewicz Sylwia , Gruchała‐Niedoszytko Marta , Bronk Marek , Dąbrowski Slawomir , Chełminska Marta , Jassem Ewa 
Published: January 2024

AbstractBackgroundMastocytosis is a rare neoplastic disease of the bone marrow associated with the proliferation and accumulation of mast cells in various internal organs, including the gastrointestinal tract. There are few studies describing the gut microbiome of patients with mastocytosis using next generation sequencing supported using traditional culture methods. The aims of the study were, firstly, the determination of nutrition habits, composition of the intestinal microflora and BMI in mastocytosis, and secondly, analysis of mastocytosis severity and symptoms depending on the composition of the intestinal microflora.MethodsThe study included 47 patients with indolent systemic mastocytosis and 18 healthy controls. All participants gave their informed consent to participate in the study. The study consisted of 3 parts: I‐clinical assessment, II ‐ examination of the intestinal microflora using the biochemical method, III ‐ 16S rRNA sequencing.ResultsThe nutrition habits and BMI of mastocytosis patients were similar to controls; however, most patients with mastocytosis had a low dietary vitamin and mineral content. As many as 94.5% of patients had too little fiber intake and mineral content. The most common cause of the abnormal stool test result with traditional culture was a titer of E. coli <106. The low richness of microbiota species indicated by the Simpson index was observed in mastocytosis, p = 0.04. There were no significant differences in the composition of the intestinal microflora depending on the type of mastocytosis; however, the tryptase level correlated with the amount of Suterella, Barnesiellaceae, Eubacterium, Odoribacter, and Anaerostipes.ConclusionsThe nutritional habits and BMI of mastocytosis patients are similar to the general population, except for too little fiber intake and mineral content. The gastrointestinal symptoms of mastocytosis patients may be related to the low richness of microbiota species and the amount of Suterella, Barnesiellaceae, Eubacterium, Odoribacter, Anaerostipes, which correlated with tryptase levels.
Targeted multiparametric magnetic resonance imaging/transrectal ultrasound‐guided (mpMRI/TRUS) fusion prostate biopsy versus systematic random prostate biopsy: A comparative real‐life study
Authors: Pham Trang H. N. , Schulze‐Hagen Maximilian F. , Rahnama'i Mohammad S. 
Published: January 2024

AbstractBackgroundPatients with suspected prostate cancer usually undergo transrectal ultrasound‐guided (TRUS) systematic biopsy, which can miss relevant prostate cancers and lead to overtreatment.AimsThe aim of this study was to evaluate the detection rate for prostate cancer in MR‐guided targeted biopsy (TB) and systematic biopsy (SB) in comparison with mpMRI of the prostate.Methods and resultsThree hundred and eight men who underwent mpMRI due to elevated PSA values between 2015 and 2020 were studied at university hospital Aachen, Germany. MRI‐images were divided into cohorts with suspicious findings (PI‐RADS ≥ 3) and negative findings (PI‐RADS < 3). In patients with PI‐RADS ≥ 3 TB combined with SB was performed. A part of this group underwent RP subsequently. In patients with PI‐RADS < 3 and clinical suspicion SB was performed. In the PI‐RADS ≥ 3 group (n = 197), TB combined with SB was performed in 194 cases. Three cases were lost to follow‐up. Biopsy yielded 143 positive biopsies and 51 cases without carcinoma. TB detected 71% (102/143) and SB 98% (140/143) of the overall 143 carcinoma. Overall, 102 carcinomas were detected by TB, hereof 66% (67/102) clinically significant (Gleason ≥ 3+4) and 34% (35/102) clinically insignificant carcinoma (Gleason 3+3). SB detected 140 carcinomas, hereof 64% (90/140) csPCA and 36% (50/140) nsPCA. Forty‐one of the overall 143 detected carcinoma were only found by SB, hereof 46% (19/41) csPCA and 54% (22/41) nsPCA. Tumor locations overlapped in 44% (63/143) between TB and SB. In 25% (36/143), SB detected additional tumor foci outside the target lesions. 70/143 patients subsequently underwent RP. The detection of tumor foci was congruent between mpMRI and prostatectomy specimen in 79% (55/70) of cases. Tumor foci were mpMRI occult in 21% (15/70) of cases. In the group with negative mpMRI (n = 111), biopsy was performed in 81 cases. Gleason ≥ 3+4 carcinoma was detected in 7% and Gleason 3+3 in 24% cases.ConclusionThere was a notable number of cases in which SB detected tumor foci that were mpMRI occult and could have been missed by TB alone. Therefore, additional systematic random biopsy is still required. A supplemental random biopsy should be considered depending on the overall clinical suspicion in negative mpMRI.
Iridium‐based Polymeric Multifunctional Imaging Tools for Biochemistry
Authors: Sansee Anuson , Kostka Libor , Marcalíková Adéla , Kudláčová Júlia , Sedlák František , Kotrchová Lenka , Šácha Pavel , Etrych Tomáš , Kielar Filip 
Published: January 2024

AbstractHerein, we report the development of a macromolecular multifunctional imaging tool for biological investigations, which is comprised of an N‐(2‐hydroxypropyl)methacrylamide backbone, iridium‐based luminescent probe, glutamate carboxypeptidase II (GCPII) targeting ligand, and biotin affinity tag. The iridium luminophore is a tris‐cyclometalated complex based on [Ir(ppy)3] with one of its 2‐phenylpyridine ligands functionalized to allow conjugation. Synthesized macromolecular probes differed in the structure of the polymer and content of the iridium complex. The applicability of the developed imaging tools has been tested in flow cytometry (FACS) based assay, laser confocal microscopy, and fluorescence lifetime imaging microscopy (FLIM). The FACS analysis has shown that the targeted iBodies containing the iridium luminophore exhibit selective labelling of GCPII expressing cells. This observation was also confirmed in the imaging experiments with laser confocal microscopy. The FLIM experiment has shown that the iBodies with the iridium label exhibit a lifetime greater than 100 ns, which distinguishes them from typically used systems labelled with organic fluorophores exhibiting short fluorescence lifetimes. The results of this investigation indicate that the system exhibits interesting properties, which supports the development of additional biological tools utilizing the key components (iridium complexes, iBody concept), primarily focusing on the longer lifetime of the iridium emitter.
1D and 2D Coordination Polymers of Calcium with Nonsteroidal Anti‐Inflammatory Drugs: Synthesis, Crystal Structures, Hirshfeld Surfaces, Antimicrobial and Antioxidant Activities
Authors: Gacki Michał , Kafarska Karolina , Korona‐Głowniak Izabela , Schab Patrycja , Wojciechowski Jakub , Gierczak Natalia , Wolf Wojciech M. 
Published: January 2024

AbstractFour alkaline earth metal complexes of ketoprofen (Hket) and indomethacin (Hind) were synthesized and characterized: [Ca(ket)2(H2O)2]n (1), [Mg(ket)2(H2O)2] (2), [Ca(ind)2(EtOH)2]n (3), and [Mg(ind)2(EtOH)2] (4). All compounds were studied by elemental analysis (EA), flame atomic absorption spectrometry (FAAS), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). Crystal structures of 1 and 3 were determined by single crystal X‐ray diffraction technique T=100 K. The structure of 1 is dominated by a one‐dimensional coordination polymer, while 3 is formed by a two‐dimensional layer stabilized by the calcium zig‐zag chains and π⋅⋅⋅π stacking interactions. Crystal packing arrangements were characterized by fingerprint plots (FPs) that were derived from the Hirshfeld surfaces (HSs). The antioxidant and antimicrobial activities of complexes were evaluated against Gram‐positive and Gram‐negative bacteria as well as yeasts.
N‐substituted pyrrole carboxylic acid derivatives from 3,4‐dihydroxyketons
Authors: Laudage Tatjana , Hüsing Torben , Rühmann Broder , Beer Barbara , Schmermund Luca , Sieber Volker 
Published: January 2024

AbstractSince the chemical industry is largely dependent on petrol‐based feedstocks, new sources are required for a sustainable industry. Conversion of biomass to high‐value compounds provides an environmentally friendly and sustainable approach, which might be a potential solution to reduce petrol‐based starting materials. This also applies for N‐heterocycles, which are a common structural motif in natural products, pharmaceuticals and functional polymers. The synthesis of pyrroles is a well‐studied and established process. Nevertheless, most routes described are not in line with the principles of green and sustainable chemistry and employ harsh reaction conditions and harmful solvents. In this study, 3,4‐dihydroxyketons are used as excellent platform chemicals for the production of N‐substituted pyrrole‐2‐carboxylic‐ and pyrrole‐2,5‐dicarboxylic acids, as they can be prepared from glucose through the intermediate d‐glucarate and converted into pyrrolic acid derivatives under mild conditions in water. The scope of this so far unknown reaction was examined using a variety of primary amines and aqueous ammonium chloride leading to pyrrolic acid derivatives with N‐substituents like alkane‐, alkene‐, phenyl‐ and alcohol‐groups with yields up to 20 %. The combination of both, enzymatic conversion and chemical reaction opens up new possibilities for further process development. Therefore, a continuous chemo‐enzymatic system was set up by first employing an immobilized enzyme to catalyze the conversion of d‐glucarate to the 3,4‐dihydroxyketone, which is further converted to the pyrrolic acid derivatives by a chemical step in continuous flow.
Total Capital Investment of plastic recycling plants correlates with energy losses and capacity
Authors: De Tommaso Jacopo , Galli Federico , Weber Robert , Dubois Jean‐Luc , Patience Gregory S. 
Published: January 2024

AbstractPlastic pollution is a generational problem, and stakeholders are turning to chemical recycling as a potential solution. However, decision‐makers necessitate quick and reliable capital investment estimations to evaluate innovative technologies, especially in the early project stage, when limited historical data are available. To address this need, we built a database of 160+ chemical recycling plants, querying for nominal capacity, year and place of construction, total capital investment (TCI), number of long‐term jobs and opportunity of subsidies. Then, we compared conventional association of the advancement of cost engineering AACE class 5 estimation methods, with literature estimates, and commercial capital expenditure confidence intervals for pyrolysis, gasification, solvolysis, and selective dissolution. We demonstrate the unreliability of classic methods, and we propose ballpark correlations based on the plant capacity, or the energy loss. Chemical recycling plants suffer from poor economy of scale (with current technologies), and capacity is not always the best indicator for TCI estimation. Pyrolysis and gasification are energy‐driven technologies, and their TCI correlates very well (R2=0.91–0.92) with the total energy losses. Solvolysis and selective dissolution, instead, are at an earlier development stage, so cost engineers or researchers will have to accept less certain TCI vs capacity (R2=0.60).
A phase II study to evaluate the safety and efficacy of anlotinib combined with toripalimab for advanced biliary tract cancer
Authors: Zhou Mingzhen , Jin Yuncheng , Zhu Sihui , Xu Chen , Li Lin , Liu Baorui , Shen Jie 
Published: January 2024

AbstractObjectivesTo assess the safety and efficacy of anlotinib (a multi‐targeted tyrosine kinase inhibitor) combined with toripalimab (a PD‐1 monoclonal antibody) in the treatment of unresectable biliary tract cancer (BTC).MethodsIn this prospective, single‐arm, single‐centre exploratory clinical study, patients with locally progressed or metastatic BTC were included. Patients were treated with anlotinib (12 mg, PO, QD, for 2 weeks and then stopped for a week, 21 days for a cycle) and toripalimab (240 mg, IV, Q3W). The primary endpoint of the study was the objective response rate (ORR), as defined in RECIST version 1.1 criteria.ResultsIn this study, 15 BTC patients who met the criteria were enrolled. The ORR was 26.7%, the median progression‐free survival (mPFS) was 8.6 months (95% CI: 2.1–15.2), the median overall survival (mOS) was 14.53 months (95% CI: 0.8–28.2) and the disease control rate (DCR) was 87.6%. A patient with hilar cholangiocarcinoma was successfully converted after three cycles of treatment and underwent surgical resection. Furthermore, patient gene sequencing revealed that STK11 was mutated more frequently in patients with poor outcomes. In addition, patients with a CD8/Foxp3 ratio > 3 had a longer survival than those with a CD8/Foxp3 ratio ≤ 3 (P = 0.0397).ConclusionsIn patients with advanced BTC, the combination of anlotinib and toripalimab demonstrated remarkable anti‐tumor potential, with increased objective response rates (ORR), longer overall survival (OS) and progression‐free survival (PFS). Moreover, STK11 and CD8/Foxp3 may be as biomarkers that can predict the effectiveness of targeted therapy in combination with immunotherapy.
Cytomorphological comparison of ThinPrep and SurePath liquid‐based cytology in thyroid fine‐needle aspiration
Authors: Lee Wen‐Ying , Wang Hsiu‐Chu , Tien Kai‐Jen 
Published: January 2024

AbstractBackgroundThe two widely established systems for liquid‐based cytology (LBC), ThinPrep and SurePath, employ different principles. The aim of this study was to compare the cytomorphology of thyroid lesions prepared by the two techniques.MethodsWe retrospectively reviewed 44 thyroid FNA specimens prepared by LBC, including 20 ThinPrep and 22 SurePath. Cytologic diagnoses were made according to the Bethesda system and cytomorphologic parameters were evaluated.ResultsAcellular smears were significantly frequent in ThinPrep than SurePath (10% vs. 0%). Both techniques produced a clean background, well cell preservation, and not apparent cell shrinkage. ThinPrep showed significantly lower cellularity than SurePath (25% vs. 4.3%). ThinPrep produced considerable flattening and fragmented clusters, while SurePath contained larger clusters in a three‐dimensional configuration. Colloid was significantly reduced in amount and fragmented in ThinPrep, and was easily observed in SurePath. In cases of Hashimoto's thyroiditis, ThinPrep produced much less leukocytes in background than SurePath. Aggregates of fibrin and leukocytes were frequently present in 10/16 cases (62.5%) processed by ThinPrep. Air‐dry artifact at periphery of the ring was present in 6/16 cases (37.5%) processed by ThinPrep. The nuclear features of papillary carcinoma were similarly evident in both LBC preparations.ConclusionSurePath seems to be superior to ThinPrep for diagnosing benign entities based on adequate representation of colloid and lymphocytes. The cell quality of both techniques in thyroid FNA was comparable, while each method introduces its own unique cytologic artifacts related to its methodology. We should recognize the cytomorphologic alterations to avoid misinterpretations.
Detection of FG‐4592 and metabolites in equine plasma, urine and hair following oral administration
Authors: Cutler Charlotte , Viljanto Marjaana , Taylor Polly , Hincks Pamela , Habershon‐Butcher Jocelyn , Gray Bobby , Scarth James 
Published: January 2024

AbstractFG‐4592 is a hypoxia‐inducible factor inhibitor that has been approved for therapeutic use in some countries. This class of compounds can increase the oxygen carrying capacity of the blood and thus have the potential to be used as performance enhancing agents in sports. The purpose of this study was to investigate the detection of FG‐4592 and metabolites in equine plasma and mane hair following a multiple dose oral administration to two Thoroughbred racehorses, to identify the best analytical targets for doping control laboratories. Urine samples were also analysed, and the results compared to previously published urine data. Liquid chromatography‐high resolution mass spectrometry was used for metabolite identification in urine and plasma. Liquid chromatography–tandem mass spectrometry was used for full sample analysis of urine, plasma and hair samples and generation of urine and plasma profiles. FG‐4592 and a mono‐hydroxylated metabolite were detected in plasma. FG‐4592 was detected with the greatest abundance and gave the longest duration of detection, up to 312 h post‐administration, and would be the recommended target in routine doping samples. FG‐4592 was detected in all mane hair samples collected post‐administration, up to 166 days following the final dose, showing extended detection can be achieved with this matrix. To the best of the authors' knowledge, this is the first report of FG‐4592 and metabolites in equine plasma and hair samples. Urine results were consistent with the previously published data, with FG‐4592 offering the best target for detection and longest detection periods.
Natural history and outcomes in paediatric RASopathy‐associated hypertrophic cardiomyopathy
Authors: Boleti Olga , Norrish Gabrielle , Field Ella , Dady Kathleen , Summers Kim , Nepali Gauri , Bhole Vinay , Uzun Orhan , Wong Amos , Daubeney Piers E. F. , Stuart Graham , Fernandes Precylia , McLeod Karen , Ilina Maria , Ali Muhammad Najih Liaqath , Bharucha Tara , Donne Grazia Delle , Brown Elspeth , Linter Katie , Jones Caroline B. , Searle Jonathan , Regan William , Mathur Sujeev , Boyd Nicola , Reinhardt Zdenka , Duignan Sophie , Prendiville Terence , Adwani Satish , Kaski Juan Pablo 
Published: January 2024

AbstractAimsThis study aimed to describe the natural history and predictors of all‐cause mortality and sudden cardiac death (SCD)/equivalent events in children with a RASopathy syndrome and hypertrophic cardiomyopathy (HCM).Methods and resultsThis is a retrospective cohort study from 14 paediatric cardiology centres in the United Kingdom and Ireland. We included children <18 years with HCM and a clinical and/or genetic diagnosis of a RASopathy syndrome [Noonan syndrome (NS), NS with multiple lentigines (NSML), Costello syndrome (CS), cardiofaciocutaneous syndrome (CFCS), and NS with loose anagen hair (NS‐LAH)]. One hundred forty‐nine patients were recruited [111 (74.5%) NS, 12 (8.05%) NSML, 6 (4.03%) CS, 6 (4.03%) CFCS, 11 (7.4%) Noonan‐like syndrome, and 3 (2%) NS‐LAH]. NSML patients had higher left ventricular outflow tract (LVOT) gradient values [60 (36–80) mmHg, P = 0.004]. Over a median follow‐up of 197.5 [inter‐quartile range (IQR) 93.58–370] months, 23 patients (15.43%) died at a median age of 24.1 (IQR 5.6–175.9) months. Survival was 96.45% [95% confidence interval (CI) 91.69–98.51], 90.42% (95% CI 84.04–94.33), and 84.12% (95% CI 75.42–89.94) at 1, 5, and 10 years, respectively, but this varied by RASopathy syndrome. RASopathy syndrome, symptoms at baseline, congestive cardiac failure (CCF), non‐sustained ventricular tachycardia (NSVT), and maximal left ventricular wall thickness were identified as predictors of all‐cause mortality on univariate analysis, and CCF, NSVT, and LVOT gradient were predictors for SCD or equivalent event.ConclusionsThese findings highlight a distinct category of patients with Noonan‐like syndrome with a milder HCM phenotype but significantly worse survival and identify potential predictors of adverse outcome in patients with RASopathy‐related HCM.
Stoichiometric Reactions between the Rhodium/Iridium Complexes and 2‐Vinylbenzaldehyde Derivatives: Synthesis and Characterization of Rhodaindanone and Iridaindanone Complexes
Authors: Chu Zhenwei , Zheng Xuejuan , Lin Yuqi , Luo Ming , Xia Haiping 
Published: January 2024

AbstractUtilizing rhodium complexes to catalyze 2‐vinylbenzaldehyde derivatives has proven to be a valuable strategy in constructing indanone moieties. In this study, the stoichiometric reactions of Wilkinson's catalyst RhCl(PPh3)3 with 2‐vinylbenzaldehyde derivatives were investigated, leading to the formation of rhodaindanone complexes (2). Notably, the transformation of of rhoda‐1‐indanone (2 a) to rhoda‐2‐indanone (3) was achieved for the first time. In comparision, the reactivity of IrCl(PPh3)3 and Ir(CO)(CH3CN)(PPh3)2 ⋅ BF4 towards 2‐vinylbenzaldehyde derivatives affords irida‐2‐indanone complexes (4) and fused iridacycles (5), respectively. These newly prepared coumpounds represent the first metellacycles resulting from the reactions between 2‐vinylbenzaldehyde derivatives and rhodium/iridium complexes and may provide further insights into the catalytic processes involving 2‐vinylbenzaldehyde derivatives for synthesizing indanone compounds.
Structural Trends and Vibrational Analysis of N,N,N′,N′−Tetramethylmalonamide Complexes Across the Lanthanide Series
Authors: Kravchuk Dmytro V. , Wang Xiaoyu , Servis Michael J. , Wilson Richard E. 
Published: January 2024

AbstractFundamental understanding of coordination chemistry across the lanthanide series is essential for explaining chemical behavior of rare‐earth metals in complex liquid‐liquid extraction processes, which in turn affects the distribution ratios and efficacy of separations as a whole. In this work, we explore the structural trends between the lanthanides and a neutral N,N,N′,N′−tetramethylmalonamide (TMMA) ligand within four isolated families of solid‐state compounds: Ln(trans‐TMMA)2(NO3)3 Ln=La−Nd, Sm; Ln(cis‐TMMA)2(NO3)3 Ln=Eu−Tb, Er; [Ln(TMMA)3(NO3)2][Ln(TMMA)(NO3)4] Ln=Dy−Tm; Ln(Κ2‐TMMA)(iPrOH)(NO3)3 and Ln(Κ1‐TMMA)(Κ2‐TMMA)(NO3)3 Ln=Yb, Lu. Moving across the lanthanide series, we note the formation of both discrete charge‐neutral complexes, as well as charged molecular anion‐cation pairs, with variations in spatial ligand arrangement, coordination numbers, and ligand denticities. IR and Raman spectroscopy paired with DFT frequency calculations were used for an in‐depth investigation of vibrational modes unique to each structural family. The collection of isolated model compounds was also discussed in the context of liquid‐liquid separations based on reported distribution ratios from malonamide extraction.
From Phenols to Antimicrobial Phenazines: Tyrosinase‐like Catalytic Activity of a Bisguanidine Based Bis(μ‐oxido) Complex
Authors: Laurini Larissa , Conte Salvatore Marco , Hüser Karl , Cordero Paul R. F. , Núñez Ponce Heliana Michaela , Zimmer Stefanie , Lauterbach Lars , Herres‐Pawlis Sonja 
Published: January 2024

AbstractThe catalytically active center of the enzyme tyrosinase, standing out for its unusual substrate diversity, consists of a side‐on μ‐η2:η2‐peroxido complex (SP). Several ligand systems stabilizing a SP and able to mimic the catalytic activity of the enzyme towards simple phenolic substrates are known. Only a few catalytically active systems based on the isoelectronic isomer structure of SP, a bis(μ‐oxido)dicopper(III) complex (O), were investigated until now. Two years ago, we presented with the TMGbenza a hybrid guanidine based tyrosinase model system stabilizing an O species with an exceptional substrate diversity. Herein we studied the catalytic activity of another O species stabilized by the bisguanidine ligand TMG2tol. The reaction conditions for the catalytic oxygenation were optimized and a broad spectrum of phenolic substrates like naphthol, quinolinols and indolols was tested. Naturally occurring phenazine derivatives like phenazine‐1‐carboxylamide (PCN) and phenazine‐1‐carboxylic acid (PCA) show antifungal as well as antibacterial activity, functioning as biological control agents against crop disease like take‐all. Hence, the synthesized phenazines were evaluated for their potential antimicrobial activities against representative gram‐positive and gram‐negative bacteria.