bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)
Authors: Oliveira Giovana R , Gallas-Lopes Matheus , Chitolina Rafael , Bastos Leonardo M , Portela Stefani M , Stahlhofer-Buss Thailana , Gusso Darlan , Gomez Rosane , Wyse Angela TS , Herrmann Ana P , Piato Angelo 
Published: January 2024

AbstractCarbofuran (CF) is a carbamate class pesticide, widely used in agriculture for pest control in crops. On the other hand, CF is also detected as a contaminant in food and water sources. This pesticide has high toxicity in non-target organisms, and its presence in the environment poses a threat to the ecosystem. Research has revealed that this pesticide acts as an inhibitor of acetylcholinesterase (AChE), inducing an accumulation of acetylcholine in the brain. Consequently, this leads to the emergence of a cholinergic syndrome and various detrimental effects on human health. Nonetheless, our understanding of CF impact on the central nervous system remains elusive. Therefore, this study aimed to investigate the effects of CF on behavioral and neurochemical parameters in adult zebrafish. The animals were exposed for 96 hours to different concentrations of CF (5, 50, and 500 µg/L) and subjected to behavioral assessments in the novel tank test (NTT) and social preference test (SPT). Subsequently, they were euthanized, and their brains were used to evaluate neurochemical markers associated with oxidative stress and AChE levels. In the NTT and SPT, CF did not alter the evaluated behavioral parameters. Furthermore, CF did not affect the levels of AChE, non-protein sulfhydryl groups, and thiobarbituric acid reactive species in the zebrafish brain. However, further research is needed regarding the environmental exposure of this compound to non-target organisms.
Reduction of chemokine CXCL9 expression by omega-3 fatty acids via ADP-ribosylhydrolase ARH3 in MIN6 insulin-producing cells
Authors: You Youngki , Sarkar Soumyadeep , Deiter Cailin , Elliott Emily C. , Nicora Carrie D. , Mirmira Raghavendra G. , Sussel Lori , Nakayasu Ernesto S. 
Published: January 2024

AbstractType 1 diabetes (T1D) results from the autoimmune destruction of the insulin producing β cells of the pancreas. Omega-3 fatty acids protect β cells and reduce the incident of T1D. However, how omega-3 fatty acids act on β cells is not well understood. We have shown that omega-3 fatty acids reduce pro-inflammatory cytokine-mediated β-cell apoptosis by upregulating the expression of the ADP-ribosylhydrolase ARH3. Here, we further investigate the β-cell protection mechanism by ARH3 by performing siRNA of its geneAdprhl2in MIN6 insulin-producing cells followed by treatment with a cocktail of the pro-inflammatory cytokines IL-1β + IFN-γ + TNF-α, and proteomics analysis. ARH3 regulated proteins from several pathways related to the nucleus (splicing, RNA surveillance and nucleocytoplasmic transport), mitochondria (metabolic pathways) and endoplasmic reticulum (protein folding). ARH3 also regulated the levels of cytokine-signaling proteins related to the antigen processing and presentation, and chemokine-signaling pathway. We further studied the role of ARH in regulating the chemokine CXCL9. We confirmed that ARH3 reduces the cytokine-induced expression of CXCL9 by ELISA. We also found that CXCL9 expression is regulated by omega-3 fatty acids. In conclusion, we showed that omega-3 fatty acids regulate CXCL9 expression via ARH3, which might have a role in protecting β cells from immune attack and preventing T1D development.
Metabolic resistance of Aβ3pE-42, target epitope of the anti-Alzheimer therapeutic antibody, donanemab
Authors: Iwata Nobuhisa , Tsubuki Satoshi , Takamura Risa , Watamura Naoto , Kakiya Naomasa , Fujioka Ryo , Mihira Naomi , Sekiguchi Misaki , Watanabe-Iwata Kaori , Kamano Naoko , Matsuba Yukio , Mann David M.A. , Robinson Andrew C. , Hashimoto Shoko , Sasaguri Hiroki , Saito Takashi , Higuchi Makoto , Saido Takaomi C. 
Published: January 2024

AbstractThe amyloid β peptide (Aβ) starting with pyroglutamate (pE) at position 3 and ending at position 42 (Aβ3pE-42) is a dominant species that accumulates in the Alzheimer’s disease (AD) brain. Consistently, a therapeutic antibody raised against this species, donanemab, has been shown to be effective in recent clinical trials. While the primary Aβ species produced physiologically is Aβ1-40/42, an explanation for how and why this physiological Aβ is converted to the pathological form has remained elusive. The conversion of Aβ1-42 to Aβ3pE-42 is likely to take place after deposition of Aβ1-42 given that Aβ3pE-42 plaques arise significantly later than Aβ1-42 deposition in the brains of singleAppknock-in and APP-transgenic mice. Here, we present experimental evidence that accounts for the aging-associated Aβ3pE-42 deposition: [1] Aβ3pE-42 is metabolically more stable than other AβX-42 species; [2] Deficiency of neprilysin (NEP), the major Aβ-degrading enzyme, induces a relatively selective deposition of Aβ3pE-42 in APP-Tg mice. [3] Aβ3pE-42 deposition always colocalizes with cored plaques in both APP-Tg and App knock-in mouse brains; [4] Aβ3E-42, an immediate precursor of Aβ3pE-42, as well as Aβ2A-42 and Aβ4F-42 are more short-lived than Aβ1-42in vivo, indicating that simple N-terminal truncation that can arise enzymatically or spontaneously makes AβX-42 easier to catabolize. Consistently, newly generated knock-in mice,AppNL-(ΔDA)-FandAppNL-(ΔDA)-Q-F, showed no detectable Aβ pathology even after aging, indicating that the Aβ3E-42 and Aβ3Q-42 species are extremely labile to thein vivocatabolic system and that the E/Q cyclase activity present in mouse brain is insufficient for Aβ3pE-42 generation. In addition, a deficiency of NEP facilitated Aβ3pE-42 deposition. Of note, we identified a trace amount of Aβ3pE-42 and its immediate precursor, Aβ3E-42, in the insoluble fraction of NEP-deficient APP-Tg mouse brains. Aβ3pE-42 is thus likely to be a probabilistic by-product of Aβ1-42 metabolism that selectively accumulates over a long-time range of brain aging. It is likely produced in the solid state or at the solid-liquid interface. Our findings suggest that anti-Aβ therapies will probably be most effective if given before Aβ3pE-42 deposition takes place.
Charting the Spatial Transcriptome of the Human Cerebral Cortex at Single-Cell Resolution
Authors: Wei Songren , Luo Meng , Wang Pingping , Chen Rui , Jin Xiyun , Xu Chang , Lin Xiaoyu , Xu Zhaochun , Liu Hongxin , Huang Peng , Xu Jiangping , Jiang Qinghua 
Published: January 2024

AbstractIn our pursuit of creating a comprehensive human cortical atlas to understand human intelligence, we examined the single-nuclei transcriptomes of 307,738 cells alongside spatial transcriptomics data from 46,948 VISIUM spots and 1,355,582 Stereo cells. Atlases reveal distinct expression patterns and spatial arrangements of cortical neural cell types. Glutamatergic neurons exhibit precise laminar patterns, often mirroring expression patterns in adjacent cortical regions. Overlaying our atlas with functional networks delineated substantial correlations between neural cell types and cortical region function. Notably, regions involved in processing sensory information (pain) display a pronounced accumulation of extratelencephalic neurons. Additionally, our atlas enabled precise localization of the thicker layer 4 of the visual cortex and an in-depth study of the stabilize the subplate structure, known as layer 6b, revealed specific marker genes and cellular compositions. Collectively, our research sheds light on the cellular foundations of the intricate and intelligent regions within the human cortex.
Isoform-level profiling of m6A epitranscriptomic signatures in human brain
Authors: Gleeson Josie , Madugalle Sachithrani U. , McLean Catriona , Bredy Timothy W. , De Paoli-Iseppi Ricardo , Clark Michael B. 
Published: January 2024

ABSTRACTThe RNA modification N6-methyladenosine (m6A) is highly abundant in the human brain and implicated in neuropsychiatric and neurodegenerative disorders. However, most techniques for studying m6A cannot resolve modifications within RNA isoforms and we lack an isoform-level map of m6A sites in the brain. Profiling m6A within isoforms is therefore a critical step towards understanding the complex mechanisms that underpin brain function and disease. Oxford Nanopore direct RNA sequencing (DRS) can quantify isoform expression, modifications and polyA tail lengths, enabling simultaneous investigation of the transcriptome and epitranscriptome. We applied DRS to three post-mortem human brain regions: prefrontal cortex, caudate nucleus and cerebellum. We identified 57,000 m6A sites within 15,000 isoforms and estimated that >27% of mRNA molecules contained an m6A modification. Our results revealed both isoform- and brain-region-specific patterning of m6A modifications and polyA tail lengths. The prefrontal cortex exhibited a distinctive profile of specifically modified isoforms enriched in excitatory neuron cell types and also had the highest proportion of previously unannotated m6A sites. A population of isoforms were hypermodified with m6A and were associated with excitatory neuron cell types in all three brain regions. We also discovered >15k differentially expressed isoforms, >2k differentially modified m6A sites and 566 isoforms with differential polyA lengths between brain regions. Our study demonstrates the utility of DRS for investigating multiple features of RNA isoforms in the brain and provides new insights into brain region specificity and functioning with implications for neurological development and disease.
PI(4,5)P2role in Transverse-tubule membrane formation and muscle function
Authors: Fujita Naonobu , Girada Shravan , Vogler Georg , Bodmer Rolf , Kiger Amy A. 
Published: January 2024

AbstractTransverse (T)-tubules – vast, tubulated domains of the muscle plasma membrane – are critical to maintain healthy skeletal and heart contractions. How the intricate T-tubule membranes are formed is not well understood, with challenges to systematically interrogate in muscle. We established the use of intact Drosophila larval body wall muscles as an ideal system to discover mechanisms that sculpt and maintain the T-tubule membrane network. A muscle-targeted genetic screen identified specific phosphoinositide lipid regulators necessary for T-tubule organization and muscle function. We show that aPI4KIIIα-Skittles/PIP5Kpathway is needed for T-tubule localized PI(4)P to PI(4,5)P2synthesis, T-tubule organization, calcium regulation, and muscle and heart rate functions. Muscles deficient forPI4KIIIαorAmphiphysin, the homolog of humanBIN1, similarly exhibited specific loss of transversal T-tubule membranes and dyad junctions, yet retained longitudinal membranes and the associated dyads. Our results highlight the power of live muscle studies, uncovering distinct mechanisms and functions for sub-compartments of the T-tubule network relevant to human myopathy.SummaryT-tubules – vast, tubulated domains of the muscle plasma membrane – are critical to maintain skeletal and heart contractions. Fujitaet al. establish genetic screens and assays in intact Drosophila muscles that uncover PI(4,5)P2regulation critical for T-tubule maintenance and function.Key FindingsPI4KIIIαis required for muscle T-tubule formation and larval mobility.API4KIIIα-Sktlpathway promotes PI(4)P and PI(4,5)P2function at T-tubules.PI4KIIIαis necessary for calcium dynamics and transversal but not longitudinal dyads.Disruption of PI(4,5)P2function in fly heart leads to fragmented T-tubules and abnormal heart rate.
Semivisible dark photon in a model with vectorlike leptons for the
(g−2)e, μ
and
W
-boson mass anomalies
Authors: Abdallah Waleed , Ashry Mustafa , Kawamura Junichiro , Moursy Ahmad 
Published: January 2024

We propose a model that realizes a semivisible dark photon which can contribute to the anomalous magnetic moment (g−2) of both electron and muon. In this model, the electron g−2 is deviated from the Standard Model (SM) prediction by the 1-loop diagrams involving the vectorlike leptons, while that of muon is deviated due to a nonvanishing gauge kinetic mixing with photons. We also argue that the W-boson mass can be deviated from the SM prediction due to the vectorlike lepton loops, so that the value obtained by the CDF II experiment can be explained. Thus, this model simultaneously explains the recent three anomalies in g−2 of electron and muon as well as the W-boson mass. The constraints on the O(1)  GeV dark photon can be avoided because of the semivisible decay of the dark photon, A′→2N→2ν2χ→2ν4e, where N is a SM singlet vectorlike neutrino and χ is a CP-even Higgs boson of the U(1)′ gauge symmetry.

Published by the American Physical Society
2024
Supernovalike explosions of massive rotating stars from disks surrounding a black hole
Authors: Fujibayashi Sho , Lam Alan Tsz-Lok , Shibata Masaru , Sekiguchi Yuichiro 
Published: January 2024

We perform a new general-relativistic viscous-radiation hydrodynamics simulation for supernovalike explosions associated with stellar core collapse of rotating massive stars to a system of a black hole and a massive torus, paying particular attention to large-mass progenitor stars with the zero-age main-sequence mass of MZAMS=20, 35, and 45M⊙ of Aguilera-Dena [ ]. Assuming that a black hole is formed in a short timescale after the onset of the stellar collapse, the new simulations are started from initial data of a spinning black hole and infalling matter that self-consistently satisfy the constraint equations of general relativity. It is found that, with a reasonable size of the viscous parameter, the supernovalike explosion is driven by the viscous heating effect in the torus around the black hole, irrespective of the progenitor mass. The typical explosion energy and ejecta mass for the large-mass cases (MZAMS=35 and 45M⊙) are ∼1052  erg and ∼5M⊙, respectively, with Ni56 mass larger than 0.15M⊙. These are consistent with the observational data of stripped-envelope and high-energy supernovae such as broad-lined type Ic supernovae. This indicates that rotating stellar collapses of massive stars to a black hole surrounded by a massive torus can be a central engine for high-energy supernovae. By artificially varying the angular velocity of the initial data, we explore the dependence of the explosion energy and ejecta mass on the initial angular momentum and find that the large explosion energy ∼1052  erg and large Ni56 mass ≥0.15M⊙ are possible only when a large-mass compact torus with mass ≳1M⊙ is formed.

Published by the American Physical Society
2024
Revisiting the delight–loyalty link in a retail banking context – an emerging market perspective
Authors: Roberts-Lombard Mornay , Makanyeza Charles , Jaiyeoba Olumide , Svotwa Tendai Douglas 
Published: January 2024

PurposeThis study uses relationship marketing theory to explore affective and calculative commitment as mediators in the delight–loyalty link. Furthermore, it investigates the role of perceived employee service delivery skills, perceived value and trust in the relationships between delight, affective commitment, calculative commitment and loyalty.Design/methodology/approachA descriptive research approach was applied, and the data were collected from 332 retail banking customers in an emergent market who are overall satisfied with their bank. A self-administered questionnaire collected data from 332 respondents who adhered to the stipulated requirements to participate in the study. These respondents were selected through purposive and convenience sampling. The constructs’ interrelationships were analysed via structural equation modelling. The measurement and structural models were also assessed.FindingsAffective and calculative commitment and delight impact loyalty. Both affective commitment and calculative commitment were found to mediate the relationship between delight and customer loyalty.Research limitations/implicationsThe study enhances an understanding of the role of affective and calculative commitment in strengthening the delight–loyalty link from a relationship marketing theory perspective.Practical implicationsThe study provides guidance to the retail banking industry in emerging markets on the importance of affective and calculative commitment in strengthening the delight–loyalty link. It further informs retail banks of the need to provide banking customers with products and service value that exceed their expectations to strengthen their future commitment and loyalty to their bank.Originality/valueGuided by relationship marketing theory, the role of affective and calculative commitment in mediating the delight–loyalty link in an emerging market context is uncovered.
Environmental challenges and innovative responses of local agri-food systems: a theoretical approach
Authors: Collado Luis , Galaso Pablo , Menéndez María de las Mercedes , Rodríguez Miranda Adrián 
Published: January 2024

Purpose
This paper aims to analyse how local agri-food systems (LAFS), compared to other production models, can offer innovative responses to the important environmental challenges facing food production under the twin transition. These responses are more conducive to community inclusion and local development.

Design/methodology/approach
The paper combines territorial development, clusters and industrial districts literature with studies on agri-food industry environmental problems and twin transition technologies to develop an agri-food systems typology. This typology is based on a territorial approach to environmental challenges of food production and serves to illustrate the ways in which LAFS can provide innovative responses to these challenges.

Findings
The study allows to visualise the differences between LAFS and other agri-food production models, showing how the operationalisation and implementation of digitisation occur at territorial level and how rural communities are involved in the process. The theoretical proposal emphasises not assuming that technology is inherently beneficial but ensuring that its implementation is inclusive and generates social value for the communities.

Originality/value
The paper aims to enrich future research by adopting a territorial perspective to study the twin transition challenges associated with food production systems.
Does metaverse stimulate tourism prosocial behavior? A mindfulness-driven model with a psychological ownership perspective
Authors: Cai Ruiying , Wang Yao-Chin , Zhang Tingting (Christina) 
Published: January 2024

Purpose
Through a theoretical lens of psychological ownership, this study aims to investigate how technology mindfulness may stimulate metaverse tourism users’ feelings of individual psychological ownership, aesthetic value and conversational value, which in turn fosters intention to engage in prosocial behaviors.

Design/methodology/approach
The study used a scenario-based survey that allowed U.S.-based participants to create their own avatars and imagine using their avatars to explore heritage sites in the metaverse. Structural equality modeling was applied for data analysis.

Findings
The results from 357 valid responses indicate that technology mindfulness arouses tourists’ individual psychological ownership, aesthetic value, conversational value and prosocial behavioral intentions. The moderating role of biospheric value orientation on willingness to donate and intention to volunteer is investigated.

Research limitations/implications
The research sheds light on the significance of technology mindfulness, conversational value and psychological ownership perspectives in the metaverse, which have been previously overlooked. The authors used a scenario-based survey for mental stimulation due to current metaverse technology limitations.

Practical implications
The study is one of the first to explore the possibility of encouraging prosocial behaviors using metaverse-facilitated technology. The research offers guidelines to engage hospitality and tourism customers in the metaverse that can blend their virtual experiences into the real world.

Originality/value
This study represents one of the pioneering efforts to gain an in-depth understanding of the application of metaverse in triggering prosocial behavior toward heritage sites, explained via a technology mindfulness-driven model with a psychological ownership perspective.
The anatomy of exporting wineries of Argentina
Authors: Depetris Chauvin Nicolas , Villanueva Emiliano C. 
Published: January 2024

Purpose
This study aims to provide a detailed characterization of Argentinean exporting wineries using a new rich firm-level data set to understand how capabilities and business strategies differ among firms with different levels of involvement in the export market.

Design/methodology/approach
A survey was distributed among all wineries along all wine regions of Argentina; the 45-min questionnaire was answered by 230 wineries, a representative sample with a response rate of 26.3% of the total population of Argentinean wineries. The survey assessed the interaction between wineries’ dynamics and characteristics and their participation in export markets. In the comparative analysis, the results are presented by dividing the sample into four categories according to the export intensity of the wineries.

Findings
High-intensity exporting wineries in Argentina differ from other Argentinean wineries in several dimensions. In particular, the most internationalized Argentinean wineries are the most endowed with higher capabilities; they follow a specific business model emphasizing product differentiation, quality upgrading, brand building and the development of distribution channels. Exporting wineries from Argentina adopt business practices that differ from those that prevail among wineries that only target the domestic market. They have developed firm capabilities such as human capital and technology to play a critical role in quality upgrading for their participation in global wine markets.

Originality/value
To the best of the authors’ knowledge, this paper is the first to study the Argentinean exporting wineries using a firm-level sizeable representative sample.