bluuebunny/crossref_metadata_2025_split huggingface dataset:
The optimal puncture time point of prolonged occlusion flow-mediated dilatation in radial artery catheterization: a prospective observational study
Authors: Qi Wensheng , Ma Haichun , Wu Xuehan , Wei Kun , Li Yanhui 
Published: December 2023

AbstractPrevious studies have demonstrated prolonged occlusion flow-mediated dilatation (PO-FMD) could reduce cannulation failure rates and decrease radial artery pulsation loss during trans-radial coronary angiography. However, the time and degree of radial artery dilatation induced after PO-FMD were unclear. This study aimed to evaluate the degree and duration of the radial artery dilation after PO-FMD, and the time point at which the radial artery diameter is expanded to the maximum. This was a prospective observational study. According to the Chinese guideline on the primary prevention of cardiovascular diseases, 142 patients awaking from general anesthesia were divided into two groups: low-risk (LR) group and high-risk (HR) group. Firstly, the baseline radial artery diameter was measured on the left wrist using ultrasound in both groups. Subsequently, the radial artery diameters were obtained continuously at the same location for 5 min after PO-FMD. The baseline radial artery diameter, the maximum radial artery diameter, and the duration of radial artery dilation in the two groups were recorded. The time point at which the radial artery diameter is expanded to the maximum in the LR group and HR group was 26.49 ± 11.69 s and 46.27 ± 12.03 s, respectively (P < 0.01). The time of radial artery dilation and the percentage changes in arterial diameter in HR group were significantly lower than LR group (duration time: mean [mean ± standard]: 136.65 ± 31.55 s vs. 168.98 ± 33.27 s; percentage changes: median [interquartile range] 10.5 [8.6, 12.9] % vs. 15.2 [12.4, 19.0] %). In this study, the optimal puncture time point of PO-FMD in the LR group was 26 s, and in the HR group was 46 s. It would be helpful to guide the time point in radial artery catheterization after PO-FMD.Chinese Clinical Trial Registry identifier: ChiCTR2200066214.
Exploring the role of cyclodextrins as a cholesterol scavenger: a molecular dynamics investigation of conformational changes and thermodynamics
Authors: Ganjali Koli Mokhtar , Fogolari Federico 
Published: December 2023

AbstractThis study presents a comprehensive analysis of the cholesterol binding mechanism and conformational changes in cyclodextrin (CD) carriers, namely βCD, 2HPβCD, and MβCD. The results revealed that the binding of cholesterol to CDs was spontaneous and thermodynamically favorable, with van der Waals interactions playing a dominant role, while Coulombic interactions have a negligible contribution. The solubility of cholesterol/βCD and cholesterol/MβCD complexes was lower compared to cholesterol/2HPβCD complex due to stronger vdW and Coulombic repulsion between water and CDs. Hydrogen bonding was found to have a minor role in the binding process. The investigation of mechanisms and kinetics of binding demonstrated that cholesterol permeates into the CD cavities completely. Replicas consideration indicated that while the binding to 2HPβCD occurred perpendicularly and solely through positioning cholesterol's oxygen toward the primary hydroxyl rim (PHR), the mechanism of cholesterol binding to βCD and MβCD could take place with the orientation of oxygen towards both rims. Functionalization resulted in decreased cavity polarity, increased constriction tendency, and altered solubility and configuration of the carrier. Upon cholesterol binding, the CDs expanded, increasing the cavity volume in cholesterol-containing systems. The effects of cholesterol on the relative shape anisotropy (κ2) and asphericity parameter (b) in cyclodextrins were investigated. βCD exhibited a spherical structure regardless of cholesterol presence, while 2HPβCD and MβCD displayed more pronounced non-sphericity in the absence of cholesterol. Loading cholesterol transformed 2HPβCD and MβCD into more spherical shapes, with increased probabilities of higher κ2. MβCD showed a higher maximum peak of κ2 compared to 2HPβCD after cholesterol loading, while 2HPβCD maintained a significant maximum peak at 0.2 for b.
Rotavirus genotype diversity in Tanzania during Rotavirus vaccine implementation between 2013 and 2018
Authors: Michael Fausta , Mirambo Mariam M. , Lyimo Dafrossa , Salehe Abdul , Kyesi Furaha , Msanga Delfina R. , Mahamba Dina , Nyawale Helmut , Kwiyolecha Elizabeth , Okamo Bernard , Mwanyika Paul J. , Maghina Victoria , Bendera Elice , Salehe Mohammed , Hokororo Adolfine , Mwipopo Ernestina , Khamis Asha C. , Nyaki Honest , Magodi Richard , Mujuni Delphius , Konje Eveline T. , Katembo Betina , Wilillo Ritha , Mshana Stephen E. 
Published: December 2023

AbstractThe study aims to determine Rotavirus genotypes between 2013 and 2018 during implementation of ROTARIX vaccine in Tanzania. The analysis of surveillance data obtained between 2013 and 2018 was done to determine circulating genotypes after introduction of Rotarix vaccine. From 2013 to 2018, a total of 10,557 samples were collected and screened for Rotavirus using an enzyme immunoassay. A significant decrease in Rotavirus positivity (29.3% to 17.8%) from 2013 to 2018 (OR 0.830, 95% CI 0.803–0.857, P < 0.001) was observed. A total of 766 randomly selected Rotavirus positive samples were genotyped. Between 2013 and 2018, a total of 18 Rotavirus genotypes were detected with G1P [8] being the most prevalent. The G1P [8] strain was found to decrease from 72.3% in 2015 to 13.5% in 2018 while the G9P [4] strain increased from 1 to 67.7% in the same years. G2P [4] was found to decrease from 59.7% in 2013 to 6.8% in 2018 while G3P [6] decreased from 11.2% in 2014 to 4.1% in 2018. The data has clearly demonstrated that ROTARIX vaccine has provided protection to varieties of the wild-type Rotavirus strains. Continuous surveillance is needed to monitor the circulation of Rotavirus strains during this era of vaccine implementation.
Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition
Authors: Sengal Asmerom T. , Bonazzi Vanessa , Smith Deborah , Moiola Cristian P. , Lourie Rohan , Rogers Rebecca , Colas Eva , Gil-Moreno Antonio , Frentzas Sophia , Chetty Naven , Perrin Lewis , Pollock Pamela M. 
Published: December 2023

AbstractEndometrial cancer (EC) patients with metastatic/recurrent disease have limited treatment options and poor survival outcomes. Recently, we discovered the FGFR2c splice isoform is associated with poor prognosis in EC patients. Here we report the establishment of 16 EC patient-derived xenografts (PDX)-derived organoids (PDXOs) with or without FGFR2c expression. In vitro treatment of 5 EC PDXOs with BGJ398 showed significant cell death in 3 models with FGFR2c expression. PDXs with high/moderate FGFR2c expression showed significant tumour growth inhibition (TGI) following 21-day treatment with FGFR inhibitors (BGJ398 or pemigatinib) and significantly prolonged survival in 4/5 models. Pemigatinib + cisplatin combination therapy (n = 5) resulted in significant TGI and prolonged survival in one of two p53abn PDXs. All five models treated with cisplatin alone showed de novo resistance and no survival benefit. Seven-day treatment with BGJ398 revealed a significant reduction in angiogenesis and CD206 + M2 macrophages. These data collectively support the evaluation of FGFR inhibitors in a clinical trial.
Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system
Authors: Liu Shaojun , Li Dongyu , Yu Tingting , Zhu Jingtan , Semyachkina-Glushkovskaya Oxana , Zhu Dan 
Published: December 2023

AbstractThe dysfunction of microglia in the development of diabetes is associated with various diabetic complications, while traditional insulin therapy is insufficient to rapidly restore the function of microglia. Therefore, the search for new alternative methods of treating diabetes-related dysfunction of microglia is urgently needed. Here, we evaluate the effects of transcranial photobiomodulation (tPBM) on microglial function in diabetic mice and investigate its mechanism. We find tPBM treatment effectively improves insulin therapy on microglial morphology and reactivity. We also show that tPBM stimulates brain drainage system through activation of meningeal lymphatics, which contributes to the removal of inflammatory factor, and increase of microglial purinergic receptor P2RY12. Besides, the energy expenditure and locomotor activity of diabetic mice are also improved by tPBM. Our results demonstrate that tPBM can be an efficient, non-invasive method for the treatment of microglial dysfunction caused by diabetes, and also has the potential to prevent diabetic physiological disorders.
Phenotypic profiling of solute carriers characterizes serine transport in cancer
Authors: Papalazarou Vasileios , Newman Alice C. , Huerta-Uribe Alejandro , Legrave Nathalie M. , Falcone Mattia , Zhang Tong , McGarry Lynn , Athineos Dimitris , Shanks Emma , Blyth Karen , Vousden Karen H. , Maddocks Oliver D. K. 
Published: December 2023

AbstractSerine is a vital amino acid in tumorigenesis. While cells can perform de novo serine synthesis, most transformed cells rely on serine uptake to meet their increased biosynthetic requirements. Solute carriers (SLCs), a family of transmembrane nutrient transport proteins, are the gatekeepers of amino acid acquisition and exchange in mammalian cells and are emerging as anticancer therapeutic targets; however, the SLCs that mediate serine transport in cancer cells remain unknown. Here we perform an arrayed RNAi screen of SLC-encoding genes while monitoring amino acid consumption and cell proliferation in colorectal cancer cells using metabolomics and high-throughput imaging. We identify SLC6A14 and SLC25A15 as major cytoplasmic and mitochondrial serine transporters, respectively. We also observe that SLC12A4 facilitates serine uptake. Dual targeting of SLC6A14 and either SLC25A15 or SLC12A4 diminishes serine uptake and growth of colorectal cancer cells in vitro and in vivo, particularly in cells with compromised de novo serine biosynthesis. Our results provide insight into the mechanisms that contribute to serine uptake and intracellular handling.
Cotadutide promotes glycogenolysis in people with overweight or obesity diagnosed with type 2 diabetes
Authors: Parker Victoria E. R. , Robertson Darren , Erazo-Tapia Edmundo , Havekes Bas , Phielix Esther , de Ligt Marlies , Roumans Kay H. M. , Mevenkamp Julian , Sjoberg Folke , Schrauwen-Hinderling Vera B. , Johansson Edvin , Chang Yi-Ting , Esterline Russell , Smith Kenneth , Wilkinson Daniel J. , Hansen Lars , Johansson Lars , Ambery Philip , Jermutus Lutz , Schrauwen Patrick 
Published: December 2023

AbstractCotadutide is a dual glucagon-like peptide 1 and glucagon receptor agonist under development for the treatment of non-alcoholic steatohepatitis and type 2 diabetes mellitus (T2DM) and chronic kidney disease. Non-alcoholic steatohepatitis is a complex disease with no approved pharmacotherapies, arising from an underlying state of systemic metabolic dysfunction in association with T2DM and obesity. Cotadutide has been shown to improve glycaemic control, body weight, lipids, liver fat, inflammation and fibrosis. We conducted a two-part, randomized phase 2a trial in men and women with overweight or obesity diagnosed with T2DM to evaluate the efficacy and safety of cotadutide compared with placebo and liraglutide. The primary endpoints were change from baseline to day 28 of treatment in postprandial hepatic glycogen (part A) and to day 35 of treatment in fasting hepatic glycogen (part B) with cotadutide versus placebo. Secondary endpoints in part B were changes in fasting hepatic glycogen with cotadutide versus the mono glucagon-like peptide 1 receptor agonist, liraglutide, and change in hepatic fat fraction. The trial met its primary endpoint. We showed that cotadutide promotes greater reductions in liver glycogen and fat compared with placebo and liraglutide. Safety and tolerability findings with cotadutide were comparable to those of previous reports. Thus, this work provides evidence of additional benefits of cotadutide that could be attributed to glucagon receptor engagement. Our results suggest that cotadutide acts on the glucagon receptor in the human liver to promote glycogenolysis and improve the metabolic health of the liver. ClinicalTrials.gov registration: NCT03555994.
A mechanistic marker-based screening tool to predict clinical immunogenicity of biologics
Authors: Jarvi Nicole L. , Balu-Iyer Sathy V. 
Published: December 2023

Abstract
Background
The efficacy and safety of therapeutic proteins are undermined by immunogenicity driven by anti-drug antibodies. Immunogenicity risk assessment is critically necessary during drug development, but current methods lack predictive power and mechanistic insight into antigen uptake and processing leading to immune response. A key mechanistic step in T-cell-dependent immune responses is the migration of mature dendritic cells to T-cell areas of lymphoid compartments, and this phenomenon is most pronounced in the immune response toward subcutaneously delivered proteins.

Methods
The migratory potential of monocyte-derived dendritic cells is proposed to be a mechanistic marker for immunogenicity screening. Following exposure to therapeutic protein in vitro, dendritic cells are analyzed for changes in activation markers (CD40 and IL-12) in combination with levels of the chemokine receptor CXCR4 to represent migratory potential. Then a transwell assay captures the intensity of dendritic cell migration in the presence of a gradient of therapeutic protein and chemokine ligands.

Results
Here, we show that an increased ability of the therapeutic protein to induce dendritic cell migration along a gradient of chemokine CCL21 and CXCL12 predicts higher immunogenic potential. Expression of the chemokine receptor CXCR4 on human monocyte-derived dendritic cells, in combination with activation markers CD40 and IL-12, strongly correlates with clinical anti-drug antibody incidence.

Conclusions
Mechanistic understanding of processes driving immunogenicity led to the development of a predictive tool for immunogenicity risk assessment of therapeutic proteins. These predictive markers could be adapted for immunogenicity screening of other biological modalities.
A path planning algorithm fusion of obstacle avoidance and memory functions
Authors: Zheng Qingchun , Li Shubo , Zhu Peihao , Ma Wenpeng , Wang Yanlu 
Published: December 2023

AbstractIn this study, to address the issues of sluggish convergence and poor learning efficiency at the initial stages of training, the authors improve and optimise the Deep Deterministic Policy Gradient (DDPG) algorithm. First, inspired by the Artificial Potential Field method, the selection strategy of DDPG has been improved to accelerate the convergence speed during the early stages of training and reduce the time it takes for the mobile robot to reach the target point. Then, optimising the neural network structure of the DDPG algorithm based on the Long Short‐Term Memory accelerates the algorithm's convergence speed in complex dynamic scenes. Static and dynamic scene simulation experiments of mobile robots are carried out in ROS. Test findings demonstrate that the Artificial Potential Field method‐Long Short Term Memory Deep Deterministic Policy Gradient (APF‐LSTM DDPG) algorithm converges significantly faster in complex dynamic scenes. The success rate is improved by 7.3% and 3.6% in contrast to the DDPG and LSTM‐DDPG algorithms. Finally, the usefulness of the method provided in this study is similarly demonstrated in real situations using real mobile robot platforms, laying the foundation for the path planning of mobile robots in complex changing conditions.
Modelling of high frequency bearing voltage for dual‐winding permanent magnet synchronous generators
Authors: Li Zhihao , Liu Ruifang , Zhang Liangliang , Li Weili , Li Shulin , Huang Xin 
Published: December 2023

AbstractThe modelling and analysis of high‐frequency bearing voltage are of great significance to the assessment and mitigation of the electrical erosion risk in wind power systems. However, the dual‐winding permanent magnet synchronous generator (DW‐PMSG), as one of the mainstream models in wind power industry, has not been specifically analysed for its bearing voltage modelling method in the existing research. The high frequency common mode equivalent circuit model of DW‐PMSG is established, and the effect of parasitic parameters between two sets of winding on bearing voltage is analysed. A model parameters extraction method based on differential evolution algorithm is proposed, and the range of parameters is estimated by finite element simulation and test results, which improves the search efficiency and solution accuracy. The accuracy of the proposed model is verified by the comparison of simulation and experimental results. On this basis, it is possible to conduct more in‐depth research on the bearing voltage and bearing currents of DW‐PMSG, and provide theoretical basis and simulation means for the design of suppression schemes.
Short‐term prediction of wind power based on temporal convolutional network and the informer model
Authors: Wang Shuohe , Chang Linhua , Liu Han , Chang Yujian , Xue Qiang 
Published: December 2023

AbstractIn this study, a new short‐term wind power prediction model based on a temporal convolutional network (TCN) and the Informer model is proposed to solve the problem of low prediction accuracy caused by large wind speed fluctuations in short‐term prediction. First, an input feature selection method based on the maximum information coefficient is proposed after considering the problem of information interference caused by excessively large input features. A dynamic time planning method is used to select the optimal input step of historical power. Then, the combined forecasting model composed of TCN and the Informer is constructed in accordance with the numerical weather forecast and historical power data. Lastly, the pinball loss function is used to expand the prediction model into a quantile regression model, measure the effect of volatility, quantify the volatility range of prediction, and finally, obtain a deterministic prediction result. The actual measured data of wind farms in the Bohai Sea area are selected for analysis and calculation. The results show that the prediction model proposed in this study achieves better accuracy in deterministic prediction and interval prediction than the traditional model.
Control method based on real–imaginary decomposition at the switching frequency for multiple active bridge converters
Authors: Ibanez Federico M. , Shubnaya Anna , Martin Fernando 
Published: December 2023

AbstractIsolated multiple port DC/DC converters, such as the Multiple Active Bridge (MAB) converter, have many recent applications, such as interconnection between grids, isolated uninterruptible power sources (UPSs) and electric vehicle chargers. MAB converter is an attractive solution from the point of view of the hardware because of its symmetry and its capability to be extended to any number of bridges with a moderate number of components. However, the main challenge of this converter is the control method in order to achieve independent control between the different ports and to minimize recirculating currents. For that reason, three‐port power converters have been already investigated, but many improvements can be done for a larger number of ports. This paper proposes to use a Fourier decomposition for the main power signals to separate their real and imaginary parts. As the signals work at the switching frequency, this decomposition is developed with analog electronics. Based on that, a general control method for regulating the power at the different ports is presented using the first harmonic component, which delivers most of the power. In this proposal, two nested control loops ensure accuracy for the DC power flow. Simulation and experimental results validate the proposal.
Probing diffuse radio emission in bridges between galaxy clusters with uGMRT
Authors: Pignataro G. V. , Bonafede A. , Bernardi G. , Riseley C. J. , Dallacasa D. , Venturi T. 
Published: December 2023

Aims. Recent X-ray and Sunyaev–Zeldovich (SZ) observations have detected thermal emission between early-stage merging galaxy clusters. The main purpose of this work is to investigate the properties of the non-thermal emission in the interacting cluster pairs Abell 0399-Abell 0401 and Abell 21-PSZ2 G114.9.
Methods. These two unique cluster pairs have been found in an interacting state. In both cases, their connection along a filament is supported by an SZ effect detected by the Planck satellite and, in the special case of Abell 0399-Abell 0401, the presence of a radio bridge has been already confirmed by LOFAR observations at 140 MHz. Here, we analyse new high-sensitivity, wideband (250–500 MHz) uGMRT data of these two systems and describe an injection procedure to place limits on the spectrum of Abell 0399-Abell 0401 and on the radio emission between Abell 21-PSZ2 G114.9.
Results. In both cases, the low-surface-brightness diffuse emission is not detected in Band 3 (250–500 MHz). For the A399-A401 pair, we are able to constrain the steep spectral index of the bridge emission to be α > 2.2 with a 95% confidence level between 140 MHz and 400 MHz. We also detect a small patch of the bridge with a flatter spectral index, which may suggest a variable spectral index distribution across the bridge area. For the A21-PSZ2 G114.9 pair, we are able to place an upper limit on the flux density of the bridge emission with two different methods, finding at the central frequency of 383 MHz a conservative value of fu1 < 260 mJy at a 95% confidence level, and a lower value of fu2 < 125 mJy
at an 80% confidence level, based on visual inspection and a morphological criterion.
Conclusions. Our work provides a constraint on the spectrum in the bridge A399-A401 that disfavours shock acceleration as the main mechanism for the radio emission. The methods that we propose for the limits on the radio emission in the A21-PSZ2 G114.9 system represent a first step towards a systematic study of these sources.