bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_0008133 contributes to lung cancer progression by promoting glycolysis metabolism through the miR‐760/MEX3A axis
Authors: Kang Shuhong , Ni Yunfeng , Lan Ke , Lv Feng 
Published: February 2024

AbstractBackgroundLung cancer is a very common cancer with poor prognosis and high mortality. Circular RNAs (circRNAs) have been confirmed to be related to the occurrence of lung cancer, and circ_0008133 has been found to be possibly related to lung cancer.MethodsExpression of circ_0008133, miR‐760, and mex‐3 RNA binding family member A (MEX3A) messenger RNA (mRNA) was detected using quantitative real‐time polymerase chain reaction (qRT‐PCR). Cell viability, colony number, migration, and invasion were assessed using cell counting kit‐8 (CCK8), colony formation, wound healing, and transwell assays. Glucose consumption and lactate production were detected using commercial kits. Protein expression was measured using western blot. Dual‐luciferase reporter assay and RNA pull‐down assay were used to analyze the relationships between miR‐760 and circ_0008133 or MEX3A. The effects of circ_0008133 knockdown on tumor growth in vivo were examined by the nude mice expriment. Immunohistochemistry (IHC) assay analyzed Ki‐67 expression.ResultsCirc_0008133 and MEX3A were markedly boosted in lung cancer tissues and cells. Circ_0008133 knockdown decreased lung cancer cell viability, glucose consumption, lactate production, colony formation, migration, and invasion. In mechanism, circ_0008133 might positively regulate MEX3A expression by sponging miR‐760. Additionally, knockdown of circ_0008133 inhibited tumor growth in vivo.ConclusionCirc_0008133 accelerated the progression of lung cancer by promoting glycolysis metabolism through the miR‐760/MEX3A axis.
Similar toxicity potential of glyphosate and glyphosate‐based herbicide on cerebellar development after maternal exposure in rats
Authors: Ojiro Ryota , Ozawa Shunsuke , Zou Xinyu , Tang Qian , Woo Gye‐Hyeong , Shibutani Makoto 
Published: February 2024

AbstractStudies on the effects of glyphosate (GlyP) and glyphosate‐based herbicides (GBHs) on cerebellar development are extremely limited. This study examined the effects of maternal exposure to GlyP and GBH on rat cerebellar development in male offspring. From day 6 of gestation until day 21 postpartum at weaning, dams were given GlyP at 1.5% or 3.0% in diet or GBH at 1.0% in drinking water (corresponding to 0.36% GlyP). At weaning, GBH exposure was linked to increased numbers of DCX+ migrating granule cells in the cortex and TUNEL+ apoptotic cells in the internal granular layer (IGL), suggesting the disappearance of mismigrated granule cells via apoptosis. GBH also upregulated Nr4a3 and downregulated Cdk5 in the cerebellar vermis, suggesting a causal relation with the impaired granule cell development at this time. GlyP (3.0%) tended to increase in the number of DCX+ migrating granule cells in the IGL and upregulated Nr4a3 at weaning. Both compounds also upregulated genes related to granule cell migration (Astn1, Astn2, Nfia, and/or Nfix) at weaning and in adulthood, which might be an ameliorative response to delayed granule cell migration. Moreover, GBH induced Purkinje cell misalignment at weaning, which could be the result of delayed granule cell migration. In adulthood, GBH was associated with upregulation of the reelin signaling‐related genes Reln, Dab1, and Efnb1, suggesting a compensatory response to Purkinje cell misalignment. GlyP induced the same gene expression changes. These results suggest that GBH reversibly disrupts cerebellar development, primarily by targeting granule cell migration and differentiation, whereas GlyP exhibited similar toxic potential as GBH.
Cyclizine induces cytotoxicity and apoptosis in macrophages through the extrinsic and intrinsic apoptotic pathways
Authors: Lu Yin‐Che , Chiang Chen‐Yu , Hsu Yu‐Wei , Chen Chun‐Jung , Chen Wen‐Ying , Tseng Ching‐Chi , Deng Lie‐Hua , Chen Shih‐Pin , Kuan Yu‐Hsiang 
Published: February 2024

AbstractCyclizine, an over‐the‐counter and prescription antihistamine, finds widespread application in the prevention and treatment of motion sickness, encompassing symptoms such as nausea, vomiting, dizziness, along with its effectiveness in managing vertigo. However, the overuse or misuse of cyclizine may lead to hallucinations, confusion, tachycardia, and hypertension. The molecular mechanisms underlying cyclizine‐induced cytotoxicity and apoptosis remain unclear. During the 24 h incubation duration, RAW264.7 macrophages were exposed to different concentrations of cyclizine. Cytotoxicity was assessed through the lactate dehydrogenase assay. Flow cytometry employing annexin V‐fluorescein isothiocyanate and propidium iodide was utilized to evaluate apoptosis and necrosis. Caspase activity and mitochondrial dysfunction were evaluated through a fluorogenic substrate assay and JC‐1 dye, respectively. Flow cytometry employing fluorogenic antibodies was utilized to evaluate the release of cytochrome c and expression of death receptor, including tumor necrosis factor‐α receptor and Fas receptor. Western blotting was utilized to evaluate the expression of the Bcl2 and Bad apoptotic regulatory proteins. The findings unveiled from the present study demonstrated that cyclizine exerted a concentration‐dependent effect on RAW264.7 macrophages, leading to the induction of cytotoxicity, apoptosis, and necrosis. This compound further activated the intrinsic apoptotic pathway by inducing mitochondrial dysfunction, Bcl2/Bad exchange expression, cytochrome c liberation, and activation of caspases contained caspase 3, 8, and 9. Moreover, the activation of the extrinsic apoptotic pathway was observed as cyclizine induced the upregulation of death receptors and increased caspase activities. Based on our investigations, it can be inferred that cyclizine prompts cytotoxicity and apoptosis in RAW264.7 macrophages in a concentration‐dependent manner by triggering both the intrinsic and extrinsic apoptotic pathways.
Environmental surveillances of River Ogio in Edo State, Nigeria
Authors: Onobun Desmond Odiamehi , Ugbenyen Anthony Moses , Olulope Ajayi , Irobehian Young Godwin 
Published: February 2024

AbstractThe study assessed the quality of water obtained from River Ogio in Etsako West Local Government Area of Edo State, Nigeria. Using standard procedures, the water samples obtained from three points of the river were analyzed for physicochemical parameters, Total heterotrophic bacteria count and Total coliform count. With exception of upstream samples, the temperature of midstream and downstream samples was lower than the range specified by the World Health Organization (WHO). The Cadmium and Arsenic levels, Total heterotrophic bacteria count and Total coliform count were above the WHO recommended limits. A total of four genera of bacteria were isolated from the water samples. Correlational analysis revealed no significant (p > 0.05) relationship between the physicochemical parameters and bacteriological properties of water obtained from River Ogio. The water obtained from River Ogio is considered unsafe for domestic consumption due to its high heavy metal content and bacteria load.
Differentiating MCI from depression through verbal memory scores
Authors: Cankaya Seyda , Yalciner Betul , Yilmaz Melek Kandemir , Yulug Burak 
Published: February 2024

AbstractINTRODUCTIONThe present study aims to assess the differences between major depressive disorder (MDD) and mild cognitive impairment (MCI) in terms of verbal learning profile together with structural changes in the brain on magnetic resonance imaging (MRI) and to reveal predictive factors for MCI.METHODSFifty‐six patients with MDD and 31 MCI subjects were assessed using the Turkish Verbal Memory Processes Test (VMPT). Brain MRI was used to evaluate sulcal atrophy (SA), ventricular atrophy, periventricular white matter hyperintensity (WMH), subcortical WMH, basal ganglia infarct, medial temporal lobe atrophy, and infratentorial infarct scores based on the Modified Visual MRI Rating Scale (MVMRS). The symptoms of depression were evaluated with the Beck Depression Inventory in both groups. Demographic factors, VMPT scores, and MVMRS scores between MDD and MCI groups were compared. Also, potential predictors of MCI were analyzed by binary logistic regression analyses.RESULTSThe total scores of VMPT and the scores of VMPT subgroups, including immediate memory, highest learning, total learning, and delayed recall, were significantly higher in the MDD groups compared to MCI patients (Mann‐Whitney U, Student's t‐test, p < 0.05), indicating that higher scores were associated with better memory. The total MVMRS score and a subgroup of MVMRS, the SA score, were significantly higher in MCI patients compared to the MDD group, suggesting more atrophic changes and a higher burden of infarction in MCI patients. In our statistical analyses, impaired immediate memory (p < 0.001; OR = 6.002; 95% CI: 1.996−18.042), increased SA (p = 0.008; OR = 1.522; 95% CI: 1.118−2.073), and education (p = 0.028; OR = 0.84; 95% CI: 0.719−0.981) were significant predictive values obtained through backward Wald elimination in the binary logistic regression model for detecting MCI.CONCLUSIONOur findings suggest that VMPT may potentially represent a novel neuropsychiatric test that might be combined with MRI‐based morphometric evaluation methods, such as MVMRS.
Beyond Representation: Using Nonfiction Children's Literature to Address Ableism
Authors: Hollins Kara G. , Schlessinger Sarah L. 
Published: February 2024

AbstractEducators who take an inclusive, anti‐ableist stance value human variation. Teaching in pursuit of anti‐ableism requires open dialogue about aspects of human diversity, complex social identities, and the contributions of people with disabilities. In this column, we build from the rich work exploring ableism via children's literature in order to push beyond representation and into the critical work of directly confronting and disrupting ableism in the classroom.
Effect of chitosan crosslinking time on the structure and antifouling performance of polyvinylidene fluoride membrane by surface gelation‐immersion precipitation phase inversion
Authors: Sun Shuo , Xu Lang , Li Hongjun , Du Wei , Zhang Hongwei , Zuo Danying 
Published: February 2024

AbstractPolyvinylidene fluoride (PVDF) porous membrane was prepared by a two‐step method of surface gelation‐immersion precipitation phase inversion. Chitosan/acetic acid solution and glutaraldehyde aqueous solution were sequentially sprayed onto the surface of the PVDF solution film, with chitosan crosslinking and gelation occurring simultaneously on the film surface. The solution film was then immersed in a coagulation bath to obtain a modified PVDF porous membrane. The effect of the crosslinking time of chitosan and glutaraldehyde on the structure and properties of the PVDF porous membrane was discussed. The results showed that with the prolongation of crosslinking time, the surface structure of the membrane changed from a dense skin layer to a porous structure; the porosity and the mean pore size of the modified PVDF membranes increased first and then decreased, and the contact angle gradually decreased. When the crosslinking time extended to 15 min, the water flux of modified membrane (M153) reached a maximum value. BSA dynamic cyclic filtration experiment showed that the retention rate (R) of the modified membrane was significantly improved, compared to 68.3% retention rate of the blank membrane (M000), but the crosslinking time had little effect on the retention rates of the four modified membranes. The antifouling data showed that the flux recovery rate of the blank membrane was 73.0%, while the flux recovery rate of the modified membrane can reach as high as 84.40%, and the irreversible pollution rate of the blank membrane was 27.7%, while the irreversible pollution rate of the modified membrane reduced to 15.6%. These results indicated that, after surface chitosan crosslinking, the hydrophilicity and antifouling properties of PVDF membranes were improved.Practitioner Points
Modified PVDF membranes with crosslinking CS coating were prepared by a two‐step method of surface gelation‐immersion precipitation phase inversion.
‐OH groups and ‐NH2 groups of CS coating improve the hydrophilicity and the antifouling property of modified PVDF membranes.
Modified PVDF membranes had larger mean pore size and higher porosity than unmodified membrane.
Flux recovery rates of the modified membranes were higher than that of unmodified membrane.
Pollution degree, reversible pollution rate, and irreversible pollution rate of modified membranes were lower than those of unmodified membrane.
Didaktisch-methodische Aspekte der Projektentwicklung
Authors: Barton Daniel 
Published: February 2024

ZusammenfassungIn diesem Kapitel werden bedeutsame didaktisch-methodische Aspekte des geplanten Projektes theoretisch aufgearbeitet und dargestellt. Zunächst wird der mathematische Inhaltsbereich unter Bezugnahme der Bildungsstandards für das Fach Mathematik beschrieben. An den in den Bildungsstandards erläuterten fachbezogenen Kompetenzen orientiert sich die konzeptionelle Ausrichtung des geplanten Unterrichtsvorhabens. Danach wird die rahmengebende Unterrichtsmethode, das Unterrichtsprojekt, unter besonderer Berücksichtigung der Merkmale des kooperativen und selbstregulierten Lernens erläutert. Anschließend wird auf Besonderheiten im Hinblick auf Projekte im Mathematikunterricht eingegangen. Zum Abschluss dieses Kapitels werden Videos im Unterricht als weiteres grundlegendes Element des Unterrichtsprojekts thematisiert. Dazu werden sowohl die Rezeption als auch die Erstellung von Videos in Bezug auf deren Lernwirksamkeit im Bildungskontext dargestellt.
Zusammenfassung und Perspektiven
Authors: Barton Daniel 
Published: February 2024

ZusammenfassungIn diesem Kapitel werden die Ergebnisse der Datenerhebungen mit den dieser Studie zugrundeliegenden Theorien sowie dargestellten Studien in Bezug gesetzt, um Ansätze und Erklärungen zur Wirkung des durchgeführten Unterrichtsprojekts zu finden. Die Ergebnisse werden hinsichtlich der einzelnen Forschungsfragen und deren Ausdifferenzierung diskutiert.
A Metric Fixed Point Theorem and Some of Its Applications
Authors: Karlsson Anders 
Published: February 2024

AbstractA general fixed point theorem for isometries in terms of metric functionals is proved under the assumption of the existence of a conical bicombing. It is new for isometries of convex sets of Banach spaces as well as for non-locally compact CAT(0)-spaces and injective spaces. Examples of actions on non-proper CAT(0)-spaces come from the study of diffeomorphism groups, birational transformations, and compact Kähler manifolds. A special case of the fixed point theorem provides a novel mean ergodic theorem that in the Hilbert space case implies von Neumann’s theorem. The theorem accommodates classically fixed-point-free isometric maps such as those of Kakutani, Edelstein, Alspach and Prus. Moreover, from the main theorem together with some geometric arguments of independent interest, one can deduce that every bounded invertible operator of a Hilbert space admits a nontrivial invariant metric functional on the space of positive operators. This is a result in the direction of the invariant subspace problem although its full meaning is dependent on a future determination of such metric functionals.
Digitale Technologien in der Pflege – Was können sie leisten?
Authors: Wolf-Ostermann Karin , Rothgang Heinz 
Published: February 2024

ZusammenfassungDigitale Pflegetechnologien gewinnen in der Langzeitpflege zunehmend an Bedeutung. Sie umfassen alle Technologien, die mittels Vernetzung und/oder Sensorik Prozesse und/oder Produkte verändern, und schließen künstliche Intelligenz, also Verfahren, Methoden und Algorithmen, um mittels Daten zu lernen und darauf aufbauend zielorientierte Handlungen zu ermöglichen, ein. Ihre Anwendung reicht von der Förderung professioneller Zusammenarbeit über Steuerung und Verwaltung, Wissenserwerb und -weitergabe, Interaktion und Beziehung bis zur körpernahen Pflege.Digitale Pflegetechnologien haben das Potenzial, gleichzeitig die Qualität der Pflege zu erhöhen und die Arbeitsbedingungen in der Pflege zu verbessern. Allerdings stehen dem Hemmnisse auf verschiedenen Ebenen entgegen: Die Entwicklung dieser Technologien wird häufig von den technischen Möglichkeiten getrieben, sodass Produkte entstehen, die im Pflegealltag keinen konkreten Nutzen entfalten. Bei der Implementation wird nur die Bedienung geschult; es erfolgt aber keine Organisationsentwicklung zur systematischen Integration der Technologien in den Arbeitsalltag. Zudem fehlen hochwertige Evaluationen, die den tatsächlichen Nutzen im Arbeitsalltag abbilden, um so potenzielle Anwender:innen für die Technologie zu gewinnen. Schließlich ist die nachhaltige Finanzierung, insbesondere der Unterhaltung dieser Technologien, nicht gesichert.Eine gelingende Digitalisierung in der Pflege setzt daher voraus, dass Technikentwickler:innen und -anwender:innen ebenso wie Politik und Wissenschaft gemeinsam diese Hemmnisse überwinden. Das impliziert, dass Pflegende von Anfang an in den Entwicklungsprozess einbezogen sind, aber auch dass Orte geschaffen werden, in denen die Wirkung digitaler Pflegetechnologien im tatsächlichen Versorgungsgeschehen evaluiert werden kann.
Risk management during the COVID-19 crisis: insights from an exploratory case study of medium-sized family businesses
Authors: Riepl Julia , Mitter Christine , Kuttner Michael 
Published: February 2024

AbstractCurrent crises pose uncertainties and threats to family businesses (FBs), demonstrating the importance of risk management (RM). Based on an explorative case study of nine Austrian medium-sized FBs, we examine the design of RM in FBs and how the COVID-19 crisis impacts their RM practices. The findings highlight that the medium-sized FBs analyzed generally rely on both formal and informal RM, and that these structures are strongly connected to their unique stewardship culture. In the wake of the COVID-19 crisis, formal RM gained increased relevance, prompting FBs to allocate additional resources for its professional upgrading. Likewise, when confronted with heightened risks during the COVID-19 crisis, informal practices such as family bonds and close ties to employees and customers are not only reinforced but also proven highly effective, resulting in increased loyalty. The COVID-19 crisis serves as a compelling illustration of how both informal and formal RM methods have grown in strength. The synergy between these RM methods enhances risk awareness within FBs, ultimately fostering resilience during unpredictable and uncertain times.
Assessing human exposure to pesticides and mycotoxins: optimization and validation of a method for multianalyte determination in urine samples
Authors: Marín-Sáez Jesús , Hernández-Mesa Maykel , Gallardo-Ramos Jose A. , Gámiz-Gracia Laura , García-Campaña Ana M. 
Published: February 2024

AbstractHumans are exposed to an increasing number of contaminants, with diet being one of the most important exposure routes. In this framework, human biomonitoring is considered the gold standard for evaluating human exposure to chemicals. Pesticides and mycotoxins are chemicals of special concern due to their health implications. They constitute the predominant border rejection notifications for food and feed in Europe and the USA. However, current biomonitoring studies are focused on a limited number of compounds and do not evaluate mycotoxins and pesticides together. In this study, an analytical method has been developed for the determination of 30 pesticides and 23 mycotoxins of concern in urine samples. A salting-out liquid–liquid extraction (SALLE) procedure was optimized achieving recoveries between 70 and 120% for almost all the compounds and limits as lower as when QuEChERS was applied. The compounds were then determined by liquid chromatography coupled to triple quadrupole mass spectrometry. Different chromatographic conditions and analytical columns were tested, selecting a Hypersild gold aQ column as the best option. Finally, the method was applied to the analysis of 45 urine samples, in which organophosphate and pyrethroid pesticides (detection rates (DR) of 82% and 42%, respectively) and ochratoxin A and deoxynivalenol (DR of 51% and 33%, respectively) were the most detected compounds. The proposed analytical method involves the simultaneous determination of a diverse set of pesticides and mycotoxins, including their most relevant metabolites, in human urine. It serves as an essential tool for biomonitoring the presence of highly prevalent contaminants in modern society.
Graphical Abstract
Late relapse of chronic myeloid leukemia after allogeneic bone marrow transplantation points to KANSARL (KANSL1::ARL17A) alteration: a case report with insights on the molecular landscape
Authors: Fontana Diletta , Zambrotta Giovanni Paolo Maria , Scannella Antonio , Piazza Rocco , Gambacorti-Passerini Carlo 
Published: February 2024

AbstractChronic myeloid leukemia is a myeloproliferative neoplasm characterized by the presence of the Philadelphia chromosome and the consequent BCR::ABL1 oncoprotein. In the era before the introduction of tyrosine kinase inhibitors (TKIs), the only potentially curative treatment was allogeneic hematopoietic stem cell transplantation (HSCT). Here, we present the case of a patient affected by CML who experienced a relapse 20 years after allogeneic HSCT. Following relapse, the patient was treated with imatinib and bosutinib, resulting in a deep molecular response and successfully discontinued treatment. Additional analysis including whole-exome sequencing and RNA sequencing provided some insights on the molecular mechanisms of the relapse: the identification of the fusion transcript KANSL1::ARL17A (KANSARL), a cancer predisposition fusion gene, could justify a condition of genomic instability which may be associated with the onset and/or probably the late relapse of his CML.
Demographic recovery of corals at a wave-exposed reef following catastrophic disturbance
Authors: Lachs Liam , Biondi Piera , Gouezo Marine , Nestor Victor , Olsudong Dawnette , Guest James , Golbuu Yimnang 
Published: February 2024

AbstractRapid recovery of coral cover following acute disturbance has been documented on many reefs. Yet measuring coverage alone can mask shifts in community and demographic structure. Here, we quantify long-term changes in population size structure for three common genera (Acropora, Pocillopora, and Stylophora) at an eastern outer reef in Palau, Micronesia, following catastrophic loss of corals due to typhoon Bopha in 2012, based on size measurements from 3648 coral colonies. Mean colony size returned to pre-disturbance levels within 4 and 6 years for Stylophora and Pocillopora, respectively. By 2020, Pocillopora colony density far exceeded pre-disturbance levels, with rapid successful recruitment following typhoon Bopha. Despite recovery of Acropora colony density by 2020, populations remained dominated by smaller colonies. We demonstrate that full demographic recovery can occur more rapidly for pocilloporids (within 6 years) compared to Acropora which had not fully recovered by 8 years post-disturbance, possibly due to fewer annual recruitment events and larger maximum colony sizes. Our results highlight the value of demographic metrics as early indicators of recovery.