bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sharp estimates for solutions of multi‐bubbles in compact Riemann surfaces
Authors: Chen Chiun‐Chuan , Lin Chang‐Shou 
Published: August 2002

AbstractIn this paper, we consider a sequence of multibubble solutions uk of the equation

where h is a C2,β positive function in a compact Riemann surface M, and ρk is a constant satisfying limk→+∞ ρk = 8mπ for some positive integer m ≥ 1. We prove among other things that

where pk,j are centers of the bubbles of uk and λk,j are the local maxima of uk after adding a constant. This yields a uniform bound of solutions as ρk converges to 8mπ from below provided that $$\Delta_0 \log h (p_{k,j}) + 8m\pi -2K (p_{k,j}) > 0$$. It generalizes a previous result, due to Ding, Jost, Li, and Wang [18] and Nolasco and Tarantello [31], hich says that any sequence of minimizers uk is uniformly bounded if ρk > 8π and h satisfies

for any maximum point p of the sum of 2 log h and the regular part of the Green function, where K is the Gaussian curvature of M. The analytic work of this paper is the first step toward computing the topological degree of (0.1), which was initiated by Li [24]. © 2002 Wiley Periodicals, Inc.
Using solution‐focused therapy during a psychotic crisis: a case study
Authors: Rhodes John , Jakes Simon 
Published: August 2002

AbstractDuring an assessment for therapy, one patient on an acute psychiatric ward proved to be extremely difficult to interview. He believed that demons were about to visit him, and this made conversation on the topic near impossible at first. Investigation of his beliefs resulted in him getting angry. At a later date, however, he requested meeting the first author. It was then decided to try out solution‐focused therapy. Given this new start, it was then possible to begin systematic assessment of beliefs and emotional state. Over 6 months a fluctuating pattern of change emerged. Follow‐up over 2 years suggested maintenance and considerable re‐engagement with everyday activities. Beliefs were modified yet he retained notions of possible judgement by demonic forces. Copyright © 2002 John Wiley & Sons, Ltd.
A method for analysing the transient and the steady‐state oscillations in third‐order oscillators with shifting bias
Authors: Buonomo A. , Lo Schiavo A. 
Published: August 2002

AbstractWe provide an asymptotic method for systematically analysing the transient and the steady‐state oscillations in third‐order oscillators with shifting bias. The method allows us to construct the general solution of the weakly non‐linear differential equation describing these oscillators through an iteration procedure of successive approximations typical of perturbation methods. The approximation to first order is obtained solving a system of two first‐order non‐linear differential equations in the leading terms of solution (dc component and fundamental harmonic), whereby the dominant dynamics, the stationary states and their stability can be easily analysed. Unlike existing approaches, our method also enables us to determine the higher harmonics as well as the frequency shift from the system's natural frequency in the exact solution through analytical formulae. In addition, formulae for higher‐order approximations of the above quantities are determined. The proposed method is applied to a practical circuit to show its usefulness in both analysis and design problems. Copyright © 2001 John Wiley & Sons, Ltd.
Detection of the plant pathogenic bacterium Xanthomonas campestris pv. Campestris in seed extracts of Brassica sp. Applying fluorescent antibodies and flow cytometry
Authors: Chitarra L.G. , Langerak C.J. , Bergervoet J.H.W. , van den Bulk R.W. 
Published: August 2002

AbstractBackgroundXanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted plant pathogenic bacterium that causes black rot of crucifers. Seed lots and plants are screened for contamination with this pathogen using plating or serological assays. These methods, however, are time consuming and not very sensitive, respectively. Therefore, flow cytometry (FCM) was evaluated as a tool for the rapid detection and quantification of Xcc cells labeled with a mixture of specific fluorescein isothicyanate (FITC)‐monoclonal antibodies (mAb) in pure culture, in mixed cultures of Xcc with either the common saprophyte Pseudomonas fluorescens (Psf) or a nonpathogenic X. campestris isolate (Xc), and in crude seed extracts.MethodsThe mAb 18G12, conjugated with FITC, was tested at dilutions of 1:50, 1:100, 1:200, and 1:400. For mixed suspensions of Xcc and Psf, mAb 18G12 was used at a dilution of 1:100. The combination of mAbs 18G12, 2F4, and 20H6, all conjugated with FITC, was used at a dilution of 1:100 for the detection and quantification of Xcc cells in mixed suspensions containing Xcc and Xc and in crude seed extracts. The analyses were performed with a Coulter EPICS XL‐MCL flow cytometer, at low flow rate during 2 min.ResultsUsing FCM, Xcc cells labeled with FITC‐conjugated mAbs (18G12, 2F4, and 20H6) were detected and quantified rapidly at low numbers, i.e., 103 colony‐forming units per milliliter in pure and in mixed cultures with Psf. The presence of the nonpathogenic Xc in the seed extracts did not interfere with the FCM results. Xcc cells were distinguished from the cells of other organisms and from small particles present in the seed extract based on the high‐intensity fluorescence of the labeled cells.ConclusionThe application of FCM in combination with FITC‐conjugated mAbs appears to be a promising technique for the detection and quantification of Xcc cells in seed extracts of crucifers. Cytometry 47:118–126, 2002. © 2002 Wiley‐Liss, Inc.
Application of virtual microscopy in clinical cytopathology
Authors: Steinberg David M. , Ali Syed Z. 
Published: August 2002

AbstractVirtual microscopy (VM) refers to the use of an automated microscope and digital imaging technology to scan, store, and view glass slides. VM systems allow the user to view a scanned image of the entire slide at multiple magnifications on a computer screen. We tested VM to evaluate its possible utility in diagnostic cytopathology. Ten cervical‐vaginal monolayered preparations (AutoCyte™ preparation) were scanned using a BLISS™ (Bacus Laboratories Inc. Slide Scanner) system. Approximately 20–30% of the cellular area of each slide was imaged. The cases were randomly chosen to include examples ranging from benign cellular changes (BCC) to high‐grade squamous intraepithelial lesions (HSIL). The computer performed image tiling and fusing of multiple JPEG images to create a high‐quality VM slide. Six examiners (two each of cytopathologists, senior residents, and cytotechnologists) blindly evaluated the VM slides using an image server program (WebSlide Browser™ thin client software). The cytopathologic diagnoses made on the VM slide were then compared to the original glass slide diagnoses. BLISS™ took 36–100 min (avg. 58.4 min) to scan the selected fields in a glass slide with file sizes ranging from 23.1–83.6 MB. Time taken by the examiners to render a diagnosis ranged from 1–15 min (avg. 4.1 min) per case. The combined diagnostic accuracy was 98.3%. Only one case of LSIL was missed by one examiner. VM is a promising new tool, which gives a user the feel and simulated experience of an actual microscopic examination and provides a useful alternative to a glass slide in diagnostic cytopathology. Possible applications include: 1) second opinion consultation without transporting the glass slide, 2) education, 3) VM proficiency tests / board exams, and 4) telepathology. Shortcomings include 1) expensive initial setup, 2) inability to maintain an adequate focus in a thick smear with multiple levels, 3) large storage size of the VM slide, and 4) relatively long time needed to scan a slide. Diagn. Cytopathol. 25:389–396, 2001. © 2001 Wiley‐Liss, Inc.
Invasive squamous‐cell carcinoma in ThinPrep® specimens: Diagnostic clues in the cellular pattern
Authors: Clark Sarah B. , Dawson Andrea E. 
Published: August 2002

AbstractDespite the increasing utilization of the ThinPrep® Pap Test (TP), limited data exist regarding the cytomorphologic features and patterns of invasive squamous‐cell carcinoma in TP specimens. We analyzed a series of TP specimens from patients with histologically confirmed invasive squamous carcinomas of the cervix. Patients with biopsy‐proven invasive squamous‐cell carcinoma of the cervix with a TP cervical cytologic specimen within the previous 2 mo were identified. The TP slides were analyzed for overall cellularity (percent circle coverage by epithelial cells), tumor cellularity, tumor diathesis, inflammation, coexistent dysplasia, and keratinization. Tumor cellularity was defined as <5%, 5–50%, and >50% of slide cellularity. In all 13 cases that were identified, a cytologic diagnosis of either invasive squamous‐cell carcinoma or suspicious for invasive squamous‐cell carcinoma was made. In 7/13 cases (54%), epithelial cells covered <40% of the slide circle. Epithelial cells covered >40% of the slide circle in 6/13 cases (46%). Tumor cellularity covered <5% of the slide circle in 4/13 cases (31%), 5–50% in 7/13 cases (54%), and >50% in 2/13 cases (15%). A tumor diathesis was present in 12/13 cases (92%). Inflammation was absent in 1/13 cases (8%), mild in 8/13 cases (62%), moderate in 2/13 cases (15%), and severe in 1/13 cases (8%). Coexistent dysplasia was identified in 12/13 cases (92%). Keratinization was identified in 9/13 specimens (69%). In the vast majority of patients, a diagnosis of squamous‐cell carcinoma was rendered on the TP cervical specimen, despite a pattern of decreased cell coverage. It could be hypothesized that tumor diathesis and inflammation may be the etiology for decreased cellularity by blocking filter coverage by epithelial cells. This cellular pattern with diathesis in the ThinPrep® smear may be a useful clue to look carefully for diagnostic cells of squamous‐cell carcinoma. Diagn. Cytopathol. 2002;26:1–4. © 2002 Wiley‐Liss, Inc.
Cytologic features of high‐grade squamous intraepithelial lesions involving endocervical glands on ThinPrep® cytology
Authors: Selvaggi Suzanne M. 
Published: August 2002

AbstractUsage of liquid‐based cytology has resulted in better cellular preservation with enhancement of nuclear features. The purpose of this retrospective 2‐yr study (January 1999 through December 2000) was to evaluate the cellular features of endocervical gland involvement by a high‐grade squamous intraepithelial lesion (HSIL) vs. endocervical adenocarcinoma in situ (AIS) on cell samples processed by the ThinPrep® method as compared to conventional smears. Of the 97 cases of CIN III diagnosed on cytology, 52 (54%) showed surface endocervical gland involvement by CIN III and form the basis of this study. There were also six cases of endocervical AIS diagnosed on histology with prior cytology. The architectural features of HSIL involving endocervical glands and AIS were similar to those previously reported on conventional smears. A consistent finding of HSIL involving endocervical glands was the loss of central cell polarity and piling within cell groups, a finding not present in AIS. Central cell polarity was maintained in cellular groupings of AIS. In addition to the cellular feature present on conventional smears, micronucleoli were clearly visualized in cells of HSIL involving endocervical glands and prominent nucleoli were present in AIS. Apoptosis and mitoses were clearly visualized in both entities. Endocervical gland involvement by HSIL has characteristic cell patterns and features on liquid‐based/thin‐layer cytology that permit their distinction from AIS. Diagn. Cytopathol. 2002;26:181–185; DOI 10.1002/dc.10061 © 2002 Wiley‐Liss, Inc.
Utility and outcomes of fine‐needle aspiration biopsy in Hodgkin's disease
Authors: Moreland Wendy S. , Geisinger Kim R. 
Published: August 2002

AbstractWe present our 10‐year experience, including clinical utilization and outcomes, with fine‐needle aspiration biopsy (FNAB) in Hodgkin's disease (HD). Eighty‐six cases from 68 patients with HD that were evaluated by FNAB were identified over a 10‐year period. Medical records of these 68 patients were reviewed. Thirty‐seven patients with primary HD underwent 41 aspirates. A diagnosis of HD was yielded in 12 and suggested in 13 cases (sensitivity 86.2%, false‐positive 0%). Nine were diagnosed as atypical lymphoid cells, four as hyperplasia/other, and three as unsatisfactory. With these diagnoses, six patients began treatment without tissue exam. Thirty‐five patients with suspected recurrent HD underwent 45 FNABs. Recurrent HD was confirmed in 15 and suggested in 12 aspirates (sensitivity 81.3%, false‐suspicious 14.3%). With these diagnoses, 22 patients began treatment for recurrence without tissue exam. FNAB is useful both for establishing a primary diagnosis and confirming recurrence in HD and thus has a high utilization at our institution. In many instances, patients can begin therapy, negating the need for formal tissue exam. Diagn. Cytopathol. 2002;26:278–282. © 2002 Wiley‐Liss, Inc.
Role of immunocytochemistry and DNA flow cytometry in the fine‐needle aspiration diagnosis of malignant small round‐cell tumors
Authors: Brahmi Urmil , Rajwanshi Arvind , Joshi Kusum , Ganguly Nirmal Kumar , Vohra Harpreet , Gupta Subhash Kumari , Dey Pranab 
Published: August 2002

AbstractIn the present study, DNA flow cytometry (FCM) and immunocytochemistry (ICC) with a selected panel of antibodies were performed on 51 cases of malignant tumors which were referred for fine‐needle aspiration biopsy (FNAB) to our Department of Cytology for the last 2 yr. Twelve cases were diagnosed as neuroblastoma, 16 as Ewing's sarcoma, 2 as retinoblastoma, 5 as non‐Hodgkin's lymphoma (NHL), 5 as rhabdomyosarcoma, 2 as peripheral neuroectodermal tumors (PNETs), and 8 as Wilms' tumor.Eleven of 12 neuroblastomas were diploid by FCM, and 1 was aneuploid, with an S‐phase fraction (SPF) of 8.3%. Neuron‐specific enolase (NSE) was negative in 3 and positive in 8 cases of neuroblastoma, whereas neuroblastoma marker was positive in 3/11. Sixteen of 17 Ewing's sarcomas were diploid, and 1 showed tetraploid aneuploidy, with an SPF of 10.06%. Eight of 13 Ewing's sarcomas were positive for Mic‐2 gene product (Ewing's marker). All 5 NHL were positive for leukocyte‐common antigen (LCA). Three of 5 rhabdomyosarcomas were diploid, and 2 cases showed aneuploidy. Rhabdomyosarcoma showed muscle‐specific actin positivity in 4 and desmin positivity in 3 cases. All 3 cases of PNET were diploid and positive for the Mic‐2 gene product, whereas NSE and vimentin were positive in 2 cases. Both cases of retinoblastoma were diploid. Immunostaining was noncontributory in 1 case, and the other showed positivity for the retinoblastoma gene product, NSE, and chromogranin. Seven of 8 Wilms' tumors were diploid, and 1 showed aneuploid, with an SPF of 11.13%. Seven of 8 Wilms' tumors were positive for cytokeratin (CK), 5 were positive for NSE, 6 were positive for epithelial membrane antigen (EMA), and 5 were positive for vimentin.FNAB diagnosis of malignant round‐cell tumors is difficult only by light microscopy. Due to the availability of specific markers for subgrouping tumors, ICC has proved to be more useful these days, while DNA FCM has little diagnostic value, as most of them are diploid. Further ancillary studies, e.g., electron microscopy, image analysis, and other molecular investigations, are required to further categorize these tumors more precisely for better clinical management of these cases. Diagn. Cytopathol. 24:233–239, 2001. © 2001 Wiley‐Liss, Inc.
PNET‐like features of synovial sarcoma of the lung: A pitfall in the cytologic diagnosis of soft‐tissue tumors
Authors: Hummel Pascale , Yang Grace C.H. , Kumar Ashok , Cohen Jean Marc , Winkler Barbara , Melamed Jonathan , Scholes John V. , Jagirdar Jaishree 
Published: August 2002

AbstractFine‐needle aspiration (FNA) cytology of soft‐tissue tumors is evolving. As more experience is gained, we are becoming aware of potential pitfalls. We describe 2 cases of synovial sarcoma of the lung, primary and metastatic, in patients who had FNA biopsy performed on a lung mass. The cytologic smears showed extremely cellular groups of malignant small round cells, intersected by small blood vessels, with numerous loose single cells, in a background of macrophages and mature lymphocytes. The tumors displayed monomorphic cells forming rosettes and displaying occasional mitoses. A diagnosis of neuroendocrine tumor/primitive neuroepithelial tumor (PNET) was suspected. Furthermore, this suspicion was supported by immunohistochemical stains, which showed positivity for a neuroendocrine marker, Leu 7 (case 1), and for a neural marker, CD 99 (O 13 or HBA 71) (both cases); and negativity for cytokeratins (case 1). The resection specimen of case 1 had mostly tightly packed small round cells, with occasional rosettes, similar to the FNA biopsy, and focal areas composed of spindle cells, organized in a focal fibrosarcoma‐like and hemangiopericytoma‐like pattern. A balanced translocation between chromosomes X and 18, demonstrated by both karyotyping and fluorescent in situ hybridization (FISH), enabled us to make a diagnosis of synovial sarcoma, which was histologically classified as poorly differentiated. Case 2 was a metastatic biphasic synovial sarcoma of the arm, with a prominent epithelial component. Synovial sarcoma, when composed mainly of small round cells on cytologic smears, is a great mimicker of neuroendocrine/PNET tumors, with light microscopic and immunohistochemical overlap. Awareness of this potential pitfall may aid in preventing a misdiagnosis. Its recognition is of major concern, especially for the poorly differentiated variant, because it is associated with a worse prognosis. Diagn. Cytopathol. 24:283–288, 2001. © 2001 Wiley‐Liss, Inc.
Plasmacytoma of the larynx diagnosed by fine‐needle aspiration cytology: A case report
Authors: Saad Reda , Raab Stephen , Liu Yulin , Pollice Philip , Silverman Jan F. 
Published: August 2002

AbstractExtramedullary plasmacytoma is a rare lesion. The use of fine‐needle aspiration for diagnosis of plasmacytoma has been described in a few sporadic reports. To the best of our knowledge, none of these reports described the cytologic findings from plasmacytoma of the larynx. We report on a case of laryngeal plasmacytoma in a 79‐yr‐old man diagnosed by fine‐needle aspiration cytology. The patient had a history of a plasmacytoma involving the sixth thoracic vertebra diagnosed in 1996, which progressed to multiple myeloma in 1997. He received treatment in the form of local radiation to the skeletal vertebrae and chemotherapy. Two years later, the patient presented with a large neck mass. Computed tomography (CT) was done at an outside facility, and the radiologic impression was of a large right glottic carcinoma with invasion into the right thyroid cartilage. Because of the history of multiple myeloma, a fine‐needle aspiration (FNA) biopsy was performed of the laryngeal mass. Cytologic examination demonstrated atypical plasma cells arranged in a dissociative fashion, consistent with a plasmacytoma. Although there are previous surgical pathology reports of laryngeal plasmacytoma, to the best of our knowledge, this is the first report of plasmacytoma of the larynx diagnosed by FNA cytology. Diagn. Cytopathol. 24:408–411, 2001. © 2001 Wiley‐Liss, Inc.
Characterization of transgene expression and Cre recombinase activity in a panel of Thy‐1 promoter‐Cre transgenic mice
Authors: Campsall Katrina D. , Mazerolle Chantal J. , De Repentingy Yves , Kothary Rashmi , Wallace Valerie A. 
Published: August 2002

AbstractThe regulatory elements of the murine Thy1.2 gene were used to drive Cre recombinase expression in the nervous system (NS) of transgenic mice. Eleven Thy1‐Cre lines exhibited transgene expression in several regions of the central and peripheral nervous systems, including the cerebral cortex, cerebellum, spinal cord, retina, and dorsal root ganglion. Thy1‐Cre expression also resulted in region‐specific activation of Cre reporter transgenes. Although Thy‐1 expression is normally initiated in postmitotic neurons in the perinatal period, we show that the Thy‐1.2 expression cassette drives Cre expression in immature proliferative zones in the NS as early as embryonic day 11 and in non‐neural tissue. The Thy1.2 transgene cassette, therefore, does not impart transgene expression that is restricted to the NS or to postmitotic neurons within the NS. This panel of Thy1‐Cre transgenic mice, however, will be useful reagents for the ablation of genes whose transcripts are spatially or temporally restricted in the developing NS. © 2002 Wiley‐Liss, Inc.
Perinatal expression of leptin in rat stomach
Authors: Oliver Paula , Picó Catalina , De Matteis Rita , Cinti Saverio , Palou Andreu 
Published: August 2002

AbstractIt has been reported recently that the stomach can produce and store leptin and release it, both into the blood and into the gastrointestinal lumen, in response to food intake. Here, we have followed the ontogenic pattern of leptin mRNA expression and leptin levels in stomach during the perinatal period, which were compared with adults. Leptin mRNA expression was assessed by reverse transcriptase‐polymerase chain reaction, and tissue leptin content by enzyme‐linked immunosorbent assay and localised by immunohistochemistry. Leptin mRNA is expressed at low levels in rat stomach in prenatal stages. It increased from 4 to 8 hr of life in suckling rats, an increase not observed in the fasted pups, which were separated from their mothers immediately after birth. Leptin expression rose steadily after birth during the first month of life, with a marked increase from 15‐day‐old rats, followed by a parallel increase in leptin levels from day 21 of life, which was coincident with the change from suckling to a solid diet. The immunohistochemical analysis showed leptin immunoreactivity at different levels of the stomach mucosa, suggesting that during early development leptin could derive from different sources. During the pre‐ and neonatal periods, leptin is mainly located at the superficial epithelium (suggesting maternal origin from amniotic cells and mammary glandular cells, respectively). At the beginning of the chow diet, the stomach produces leptin in the glands (main source from 15 days of life), suggesting an endogenous production of the protein after that period. The present work demonstrates the expression of leptin mRNA and leptin protein in the stomach of neonate rats, and shows that the ontogenic profile of leptin appearance in the stomach during the perinatal period is probably related to the onset of suckling and to the change of diet from milk to solid chow. © 2001 Wiley‐Liss, Inc.
Longitudinal comparison of anorexia nervosa subtypes
Authors: Eddy Kamryn T. , Keel Pamela K. , Dorer David J. , Delinsky Sherrie S. , Franko Debra L. , Herzog David B. 
Published: August 2002

AbstractObjectiveTo compare patients with restricting anorexia nervosa (ANR) and binge/purge anorexia nervosa (ANBP) on measures of impulsivity, course, and outcome.MethodsOne hundred thirty‐six treatment‐seeking women with AN followed prospectively for 8–12 years were reclassified at intake as 51 ANRs and 85 ANBPs according to the DSM‐IV subtyping classification. Lifetime Axis I and Axis II disorders were assessed using structured interviews; follow‐up interviews were conducted at 6–12‐month intervals to collect weekly data on eating disorder symptomatology.ResultsWomen with ANR and ANBP did not differ on history of substance abuse, kleptomania, suicidality, or borderline personality diagnosis at intake, or on rates of recovery, relapse, or mortality. By 8 years of follow‐up, 62% of women with ANR crossed over to ANBP prospectively and only 12% of women with AN never reported regular binge/purge behaviors.ConclusionsThe findings on impulsivity, course, and outcome do not support the current subtyping system. The high crossover rate in our sample from ANR to ANBP suggests that ANR represents a phase in the course of AN rather than a distinct subtype. © 2002 by Wiley Periodicals, Inc. Int J Eat Disord 31: 191–201, 2002; DOI. 10.1002/eat.10016