bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microarray molecular mapping of horses with severe asthma
Authors: White Samuel J. , Couetil Laurent , Richard Eric A. , Marti Eliane , Wilson Philippe B. 
Published: December 2023

AbstractBackgroundSevere asthma (SA) in horses, resembling human asthma, is a prevalent, debilitating allergic respiratory condition marked by elevated allergen‐specific immunoglobulin E (IgE) against environmental proteins; however, research exploring the exposome's influence on IgE profiles is currently limited but holds paramount significance for diagnostic and therapeutic developments.AnimalsThirty‐five sports horses were analyzed, consisting of environmentally matched samples from France (5 SA; 6 control), the United States (6 SA; 6 control), and Canada (6 SEA; 6 control).MethodsThis intentional cross‐sectional study investigated the sensitization profiles of SA‐affected and healthy horses via serological antigen microarray profiling. Partial least square‐discriminant analysis (PLS‐DA) was used to identify and rank the importance of allergens for class separation (ie, affected/non‐affected) as variable influence of projection (VIP), and allergen with commonality internationally established via frequency analysis.ResultsPLS‐DA models showed high discriminatory power in predicting SA in horses from Canada (area under the curve [AUC] 0.995) and France (AUC 0.867) but poor discriminatory power in horses from the United States (AUC 0.38). Hev b 5.0101, Cyn D, Der p 2, and Rum cr were the only shared allergens across all geographical groups.Conclusions and Clinical ImportanceMicroarray profiling can identify specific allergenic components associated with SA in horses, while mathematical modeling of this data can be used for disease classification, highlighting the variability of sensitization profiles between geographical locations and emphasizing the importance of local exposure to the prevalence of different allergens. Frequency scoring analysis can identify important variables that contribute to the classification of SA across different geographical regions.
Viremia and nasal shedding for the diagnosis of equine herpesvirus‐1 infection in domesticated horses
Authors: Pusterla Nicola , Dorman David C. , Burgess Brandy A. , Goehring Lutz , Gross Margaret , Osterrieder Klaus , Soboll Hussey Gisela , Lunn David P. 
Published: December 2023

AbstractBackgroundEquine herpesvirus type 1 (EHV‐1) infection is associated with upper respiratory disease, EHM, abortions, and neonatal death.Research QuestionsAre nasal secretions a more sensitive biological sample compared to blood for the detection of EHV‐1 infection? How long is EHV‐1 detectable after primary infection by PCR?MethodsMedLine and Web of Science searches identified original peer‐reviewed reports evaluating nasal shedding and viremia using virus isolation methods or PCR published in English before October 9, 2023.ResultsSixty experimental and 20 observational studies met inclusion criteria. EHV‐1 detection frequency by qPCR in nasal secretions and blood from naturally‐infected horses with fever and respiratory signs were 15% and 9%, respectively; qPCR detection rates in nasal secretions and blood from horses with suspected EHM were 94% and 70%, respectively. In experimental studies the sensitivity of qPCR matched or exceeded that seen for virus isolation from either nasal secretions or blood. Detection of nasal shedding typically occurred within 2 days after EHV‐1 inoculation with a detection period of 3 to 7 days. Viremia lasted 2 to 7 days and was usually detected ≥1 days after positive identification of EHV‐1 in nasal secretions. Nasal shedding and viremia decreased over time and remained detectable in some horses for several weeks after inoculation.Conclusions and Clinical ImportanceUnder experimental conditions, blood and nasal secretions have similar sensitivity for the detection of EHV‐1 when horses are sampled on multiple consecutive days. In contrast, in observational studies detection of EHV‐1 in nasal secretions was consistently more successful.
Potential economic impact on U.S. aquaculture of injurious species listing of major aquaculture species
Authors: Engle Carole Ruth , van Senten Jonathan , Tuckett Quenton M. , Hill Jeffrey E. 
Published: December 2023

AbstractRegulatory changes to the Lacey Act and recent petitions to add aquaculture species in trade to the list of injurious wildlife could lead to prohibitions of interstate movement of live aquatic organisms with the potential for unintended, negative economic consequences. In this analysis, the economic impact of the potential federal prohibition of interstate transport of seven aquaculture species (blue catfish, tilapia, red swamp crawfish, grass carp, koi, guppy, and goldfish) currently in trade was assessed. Total economic impact was estimated to be $452 million (USD) annually, with a loss of 4819 jobs and a loss of $35 million in tax revenue. Effects could potentially occur on the largest sectors of U.S. aquaculture in as many as 80% of U.S. states and result in negative economic effects on as much as 21% of U.S. aquaculture farms.
Diversity gradients of terrestrial vertebrates – substantial variations about a common theme
Authors: Raz T. , Allison A. , Avila L. J. , Bauer A. M. , Böhm M. , Caetano G. H. de O. , Colli G. , Doan T. M. , Doughty P. , Grismer L. , Itescu Y. , Kraus F. , Martins M. , Morando M. , Murali G. , Nagy Z. T. , Nogueira C. de C. , Novosolov M. , Oliver P. M. , Passos P. , Pincheira‐Donoso D. , Sindaco R. , Slavenko A. , Torres‐Carvajal O. , Uetz P. , Wagner P. , Zimin A. , Roll U. , Meiri S. 
Published: December 2023

AbstractEnvironmental factors, such as temperature, precipitation, and elevation, explain most of the variation in species richness at the global scale. Nevertheless, richness patterns may have different drivers across taxa and regions. To date, a comprehensive global examination of how various factors such as climate or topography drive patterns of species richness across all terrestrial vertebrates, using the same methods and predictors, has been lacking. Recent advances in species‐distribution data allowed us to model and examine the richness pattern of all terrestrial tetrapods comprehensively. We tested the relationship between environmental and biogeographical variables and richness of amphibians (5983 species), birds (9630), mammals (5004), reptiles (8939), and tetrapods as a whole, globally, and across biogeographical realms. We studied the effects of climatic, ecological, and biogeographic drivers using generalized additive models. Richness patterns and their environmental associations varied among taxa and realms. Overall precipitation was the predominant richness predictor. However, temperature was more important in realms where both cold and warm conditions exist. In the Indomalayan realm, elevational range was very important. Richness patterns of mammals, birds, and amphibians were strongly related to precipitation whereas reptile richness was mostly associated with temperature. Our results support the universal importance of precipitation but also suggest that future global‐scaled research should incorporate other relevant variables other than climate, such as elevational range, to gain a better understanding of the richness–environment relationship. By doing so, we can further advance our knowledge of the complex relationships between biodiversity and the environment.
NIT24 and NIT29‐mediated IAA synthesis of Xanthomonas oryzae pv. oryzicola suppresses immunity and boosts growth in rice
Authors: Zhang Haimiao , Rong Zixuan , Li Yang , Yin Ziyi , Lu Chongchong , Zhao Haipeng , Kong Lingguang , Meng Lun , Ding Xinhua 
Published: December 2023

AbstractAuxin plays a pivotal role in the co‐evolution of plants and microorganisms. Xanthomonas oryzae pv. oryzicola (Xoc) stands as a significant factor that affects rice yield and quality. However, the current understanding of Xoc's capability for indole 3‐acetic acid (IAA) synthesis and its mechanistic implications remains elusive. In this study, we performed a comprehensive genomic analysis of Xoc strain RS105, leading to the identification of two nitrilase enzyme family (NIT) genes, designated as AKO15524.1 and AKO15829.1, subsequently named NIT24 and NIT29, respectively. Our investigation unveiled that the deletion of NIT24 and NIT29 resulted in a notable reduction in IAA synthesis capacity within RS105, thereby impacting extracellular polysaccharide production. This deficiency was partially ameliorated through exogenous IAA supplementation. The study further substantiated that NIT24 and NIT29 have nitrilase activity and the ability to catalyse IAA production in vitro. The lesion length and bacterial population statistics experiments confirmed that NIT24 and NIT29 positively regulated the pathogenicity of RS105, suggesting that NIT24 and NIT29 may regulate Xoc invasion by affecting IAA synthesis. Furthermore, our analysis corroborated mutant strains, RS105_ΔNIT24 and RS105_ΔNIT29, which elicited the outbreak of reactive oxygen species, the deposition of callose and the upregulation of defence‐related gene expression in rice. IAA exerted a significant dampening effect on the immune responses incited by these mutant strains in rice. In addition, the absence of NIT24 and NIT29 affected the growth‐promoting effect of Xoc on rice. This implies that Xoc may promote rice growth by secreting IAA, thus providing a more suitable microenvironment for its own colonization. In summary, our study provides compelling evidence for the existence of a nitrilase‐dependent IAA biosynthesis pathway in Xoc. IAA synthesis‐related genes promote Xoc colonization by inhibiting rice immune defence response and affecting rice growth by increasing IAA content in Xoc.
Anticancer role of lidocaine in oral squamous cell carcinoma through IGF2BP2‐mediated CAV1 stability
Authors: Wang Zhi , Zhang Lina , Wu Ting , Pan Xu , Li Le , Liu Yong 
Published: December 2023

AbstractObjectiveLidocaine, a common local anesthetic in medical practice, exhibits anticancer properties across various tumor types. In this study, we aimed to investigate the effects and mechanisms of lidocaine on oral squamous cell carcinoma.MethodsCell viability and proliferation were assessed through CCK‐8 and EdU assays. Transwell assays were used to analyze cell migration and invasion. Immunofluorescence assays were conducted to determine MMP9 levels. In vivo tumor growth was evaluated using a tumor xenograft model, and Ki67 and MMP9 levels were determined using immunohistochemistry. N6‐methyladenosine levels were assessed using dot plots and ELISA. mRNA and protein levels were examined through reverse transcription‐quantitative PCR or western blot analysis. The association between IGF2BP2 and caveolin‐1 was validated through RIP and luciferase reporter assays.ResultsLidocaine exhibited suppressive effects on the viability, migration, invasion, and tumor formation of oral squamous cell carcinoma. IGF2BP2 expression correlated with poor survival and was downregulated by lidocaine. Lidocaine reduced caveolin‐1 stability by decreasing IGF2BP2 levels. Caveolin‐1 overexpression partially reversed the suppressive effects of lidocaine on the progression of oral squamous cell carcinoma cells.ConclusionLidocaine exerts an anticancer role in oral squamous cell carcinoma via IGF2BP2‐mediated regulation of caveolin‐1 stability.
Effect of myopia‐control lenses on central and peripheral visual performance in myopic children
Authors: Wu Junqian , Li Xue , Huang Yingying , Luo Yifan , Zhang Siqi , Cui Zaifeng , Hou Fang , Bao Jinhua , Chen Hao 
Published: December 2023

AbstractPurposeTo evaluate the short‐term effects of three myopia‐control lenses, which impose peripheral myopic defocus while providing clear central vision, on central and peripheral visual performance in myopic children.MethodsTwenty‐one myopic children were enrolled in the study. Central visual performance was assessed using the quick contrast sensitivity function. Peripheral visual performance was evaluated by measuring peripheral contrast threshold and global motion perception, while subjects maintained fixation through the central portion of the lens. Single‐vision spectacle lenses (SVL), spectacle lenses with highly aspherical lenslets (HAL) and defocus‐incorporated soft contact (DISC) lenses were evaluated in random order, followed by orthokeratology (OK) lenses. All tests were performed monocularly on the right eye.ResultsThe area under the log contrast sensitivity function (AULCSF) with DISC lenses was lower than that with SVL (1.14 vs. 1.40, p < 0.001) and HAL (1.14 vs. 1.33, p = 0.001). HAL increased the temporal visual field contrast threshold compared with OK lenses (p = 0.04), and OK lenses decreased the superior visual field contrast threshold compared with that of SVL (p = 0.04) and HAL (p = 0.005). HAL also increased the peripheral coherence threshold for identifying the contraction movement compared with OK lenses (p = 0.01).ConclusionsThe short‐term use of these optical interventions for myopia control exhibited measurable differences in central and peripheral visual performance. Relevant attention could be paid to these differences, especially when children switch to different treatments. DISC lenses exhibited worse central contrast sensitivity than SVL and HAL. Imposing peripheral defocus signals did not affect children's peripheral visual performance compared with SVL. However, considering the poorer peripheral visual performance provided by HAL, OK lenses are recommended for children if there are specific demands for global scene recognition and motion perception.
Two‐incision versus three‐incision implantation technique of subcutaneous implantable cardioverter defibrillator: Systematic review and meta‐analysis of 2076 patients
Authors: Elkasaby Mohamed Hamouda , Khalefa Basma Badrawy , Yassin Mazen Negmeldin Aly , El‐Hameed Malak Mohamed Abd , Elkoumi Omar , Al Hennawi Hussam 
Published: December 2023

AbstractIntroductionThe implantable cardioverter‐defibrillator (ICD) was designed to detect and treat ventricular arrhythmias, which account for nearly half of all cardiovascular fatalities. Transvenous ICD (TV‐ICD) complications were reduced by introducing subcutaneous ICD (S‐ICD). S‐ICD can be implanted using a three (3IT)‐ or two (2IT)‐incision technique. This systematic review and meta‐analysis was conducted to compare the 3IT to the 2IT.MethodsWe searched medical electronic databases of Cochrane Central, Embase, PubMed, Scopus, and Web of Science (WOS) from the study's inception until March 8, 2023. We compared 2IT and 3IT techniques of S‐ICDs in terms of procedural, safety, and efficacy outcomes. We used Review Manager software for the statistical analysis. We calculated the risk ratio (RR) with its 95% confidence interval (CI) for dichotomous variables; and the mean difference with its 95% CI for continuous variables. We measured the heterogeneity using the chi‐squared and I‐squared tests. If the data were heterogeneous, the random‐effect (RE) model was applied; otherwise, the fixed‐effect model (FE) was used.ResultsWe included three retrospective observational studies of 2076 patients, 1209 in the 2IT group and 867 in the 3IT. There was no statistically significant difference in erosion after S‐ICD when 2IT compared with 3IT (RR = 0.27, 95% CI: [0.07, 1.02]; P = .05) (I2 = 0%, P = .90). There was no difference in risk of infection, lead dislocation, or inappropriate shock with either incision technique (RR = 0.78, 95% CI: [0.48, 1.29]; P = .34) (I2 = 0%, P = .71) and (RR = 0.37, 95% CI: [0.02, 8.14]; P = .53) (I2 = 66%, P = .05) respectively. Our meta‐analysis showed that the efficacy of both techniques is comparable; Appropriate shock (RR = 0.94, 95% CI: [0.78, 1.12]; P = .48) (I2 = 0%, P = .81) and first shock efficacy (RR = 0.89, 95% CI: [0.44, 1.82]; P = .76) (I2 = 0%, P = .87).Conclusion2IT and 3IT of S‐ICD have comparable efficacy and complication rates; however, the 3IT exposes patients to an additional incision without any additional benefits. These findings may provide clinicians with a simpler method for subcutaneous ICD implantation and likely result in improved cosmetic outcomes. Before the 2IT technique can be considered the standard of care, randomized controlled trials (RCTs) must be conducted to assess its long‐term safety and efficacy.
Component‐resolved diagnosis in childhood and prediction of asthma in early adolescence: A birth cohort study
Authors: Farraia Mariana , Mendes Francisca Castro , Sokhatska Oksana , Rama Tiago , Severo Milton , Custovic Adnan , Rufo João Cavaleiro , Barros Henrique , Moreira André 
Published: December 2023

AbstractIntroductionComponent‐resolved diagnosis (CRD) has been decisive in exploring the mechanisms of IgE sensitization, but the predictive ability to detect asthma has not been addressed. We aim to develop and evaluate the performance of a personalized predictive algorithm for asthma that integrates information on allergic sensitization using CRD.MethodsOne thousand one hundred one twenty‐five children from the Generation XXI birth cohort were randomly selected to perform a screening test for allergic sensitization and a subsample was characterized using CRD against 112 allergen components. Allergen components were analyzed using volcano plots and partial least squares (PLS) analysis. Logistic regression was performed to assess the associations between the obtained latent components (LC) and allergic outcomes (asthma, rhinitis, eczema) including other potential predictors used in previous asthma risk scores. The accuracy of the model in predicting asthma was assessed using Receiver Operating Characteristic (ROC) curve statistics.ResultsIn the PLS, the first LC was positively associated with asthma, rhinitis, and eczema. This LC was mainly driven by positive weights for Der p 1/2/23, Der f 1/2, and Fel d 1. The main components in the second LC were pollen and food allergens. History of early wheezing and parental allergy were included in the predictive model and the area under the curve improved to 0.82.ConclusionsThis is the first approach to improve the clinical applicability of CRD by combining CRD and clinical data to predict asthma at 13 years. Sensitization to distinct allergen molecules seems relevant to improve the accuracy of asthma prediction models.
Immediate postdelivery airway management of neonates with prenatally diagnosed micrognathia: A retrospective observational study
Authors: Berger Jessica A. , Nelson Olivia , Staben James , Javia Luv R. , Simpao Allan F. , Khalek Nahla , Oliver Edward R. , Adzick N. Scott , Lin Elaina E. 
Published: December 2023

AbstractIntroductionMicrognathic neonates are at risk for upper airway obstruction, and many require intubation in the delivery room. Ex‐utero intrapartum treatment is one technique for managing airway obstruction but poses substantial maternal risks. Procedure requiring a second team in the operating room is an alternative approach to secure the obstructed airway while minimizing maternal risk.The aim of this study was to describe the patient characteristics, airway management, and outcomes for micrognathic neonates and their mothers undergoing a procedure requiring a second team in the operating room at a single quaternary care children's hospital.MethodsThis was a retrospective descriptive study. Subjects had prenatally diagnosed micrognathia and underwent procedure requiring a second team in the operating room between 2009 and 2021. Collected data included infant characteristics, delivery room airway management, critical events, and medications. Follow‐up data included genetic testing and subsequent procedures within 90 days. Maternal data included type of anesthetic, blood loss, and incidence of transfusion.ResultsFourteen deliveries were performed via procedure requiring a second team in the operating room during the study period. 85.7% were male, and 50% had a genetic syndrome. Spontaneous respiratory efforts were observed in 93%. Twelve patients (85.7%) required an endotracheal tube or tracheostomy. Management approaches varied. Medications were primarily a combination of atropine, ketamine, and dexmedetomidine. Oxygen desaturation was common, and three patients experienced bradycardia. There were no periprocedural deaths. Follow‐up at 90 days revealed that 78% of patients underwent at least one additional procedure, and one patient died due to an unrelated cause.All mothers underwent cesarean deliveries under neuraxial anesthesia. Median blood loss was 700 mL [IQR 700 mL, 800 mL]. Only one mother required a blood transfusion for pre‐procedural placental abruption.DiscussionProcedure requiring a second team in the operating room is a safe and effective approach to manage airway obstruction in micrognathic neonates while minimizing maternal morbidity.ConclusionsThough shown to be safe and effective, more data are needed to support the use of procedure requiring a second team in the operating room as an alternative to ex‐utero intrapartum treatment for micrognathia outside of highly specialized maternal–fetal centers.
SpeedFlower: a comprehensive speed breeding protocol for indica and japonica rice
Authors: Kabade Pramod Gorakhanath , Dixit Shilpi , Singh Uma Maheshwar , Alam Shamshad , Bhosale Sankalp , Kumar Sanjay , Singh Shravan Kumar , Badri Jyothi , Varma Nadimpalli Rama Gopala , Chetia Sanjay , Singh Rakesh , Pradhan Sharat Kumar , Banerjee Shubha , Deshmukh Rupesh , Singh Suresh Prasad , Kalia Sanjay , Sharma Tilak Raj , Singh Sudhanshu , Bhardwaj Hans , Kohli Ajay , Kumar Arvind , Sinha Pallavi , Singh Vikas Kumar 
Published: December 2023

SummaryTo increase rice yields and feed billions of people, it is essential to enhance genetic gains. However, the development of new varieties is hindered by longer generation times and seasonal constraints. To address these limitations, a speed breeding facility has been established and a robust speed breeding protocol, SpeedFlower is developed that allows growing 4–5 generations of indica and/or japonica rice in a year. Our findings reveal that a high red‐to‐blue (2R > 1B) spectrum ratio, followed by green, yellow and far‐red (FR) light, along with a 24‐h long day (LD) photoperiod for the initial 15 days of the vegetative phase, facilitated early flowering. This is further enhanced by 10‐h short day (SD) photoperiod in the later stage and day and night temperatures of 32/30 °C, along with 65% humidity facilitated early flowering ranging from 52 to 60 days at high light intensity (800 μmol m−2 s−1). Additionally, the use of prematurely harvested seeds and gibberellic acid treatment reduced the maturity duration by 50%. Further, SpeedFlower was validated on a diverse subset of 198 rice accessions from 3K RGP panel encompassing all 12 distinct groups of Oryza sativa L. classes. Our results confirmed that using SpeedFlower one generation can be achieved within 58–71 days resulting in 5.1–6.3 generations per year across the 12 sub‐groups. This breakthrough enables us to enhance genetic gain, which could feed half of the world's population dependent on rice.