bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the Social Tabooisation of Asexuality: The Underpinnings of Anti-Asexual Bias
Authors: Iraklis Grigoropoulos 
Published: October 2023

Abstract
Introduction
Anti-asexual bias has received limited but growing public and academic attention. Examining prejudice towards asexuals expands the depth of intergroup and intragroup relation research.

Methods
The current study is aimed at clarifying anti-asexuality bias by examining attitudes towards asexual individuals with a multi-item measure in Greek culture. An exploratory cross-sectional study was conducted between April 4 and May 4, 2021, via an online survey. One hundred and eighty-seven undergraduate students participated in the current study. Bivariate correlation was used to explore the associations between variables of interest. Next, hypotheses were examined by performing a bootstrapping analysis for parallel multiple mediation models.

Results
The findings of this study support the role of context-related socio-cultural (religiosity, political positioning) and social-psychological factors (adherence to social norms) in predicting participants’ anti-asexual bias.

Conclusions
This study draws attention to the stigmatization of asexuality. It warns professionals, policymakers, and social agents about the dominant sexually normative socio-cultural context that may negatively affect asexuals’ lives.

Policy Implications
Providing information about the supporting base of outgroup dislike might be a way of promoting social change. Stakeholders and professionals who influence people’s lives (educators, health professionals) should be aware of possible stigmatization to no further stigmatize asexual individuals, ensuring they do not internalize and project these stereotypical assumptions.
Chronic effects induced by zinc oxide nanoparticles against larvae of the northern house mosquito Culex pipiens (Diptera: Culicidae)
Authors: Ibrahim Ahmed M. A. , Alfuhaid Nawal Abdulaziz , Thabet Marwa Adel , Ali Ali Mohamed 
Published: October 2023

AbstractIt is estimated that up to a million person are subject to death every year from mosquito-borne diseases. To avoid the epidemic situations arising from mosquito-borne diseases, it is necessary to reduce the mosquito populations. Challenges against efficient mosquito management are mainly related to emergence of insecticide resistance leading to increased need for the development of alternative methods. Ideal insecticides cause permanent impacts on the target insects in order to ensure powerful insecticidal effect. This study hypothesized that the impact of zinc oxide nanoparticles (ZnONPs) on the larvae of Culex pipiens Linnaeus, 1758 (Diptera: Culicidae) mosquito is irreversible and chronic. The first instar C. pipiens larvae were treated with a sublethal concentration (LC20, 0.24 g/L) of ZnONPs for 72 h and then allowed to recover for additional 72 h. Following the recovery period, the changes in zinc accumulation, growth rate, gut ultrastructure, biochemical changes in the hydrogen peroxide, antioxidant and detoxification enzymes were recorded and compared between recovered larvae and untreated (control). Recovered larvae showed significant increase in the accumulated zinc and reduced growth rate by about 50% compared to untreated (control). Furthermore, the ultrastructure of the alimentary canal epithelium showed several forms of pathological signs in different parts of the midgut of recovered larvae. Treatment with ZnONPs induced oxidative stress (OS) which appeared in the form of significant increase in hydrogen peroxide concentration. In response to OS, insects activate the detoxification system to get rid of the toxic nanoparticles. The detoxification enzyme alkaline phosphatase (ALP) and the antioxidant enzyme glutathione peroxidase (GPX) were inhibited while superoxide dismutase (SOD) was activated against ZnONPs toxicity. Additionally, recovered larvae didn’t show differences in the catalase activity from untreated control. These results verified that ZnONPs induce chronic impacts on C. pipiens larvae suggesting that it can be used in their management via direct application in standing water sources including accumulated rains and swimming pools.
Justice and Empowerment Through Digital Health: Ethical Challenges and Opportunities
Authors: Nickel Philip J. , Loosman Iris , Frank Lily , Vinnikova Anna 
Published: October 2023

AbstractThe proposition that digital innovations can put people in charge of their health has been accompanied by prolific talk of empowerment. In this paper we consider ethical challenges and opportunities of trying to achieve justice and empowerment using digital health initiatives. The language of empowerment can misleadingly suggest that by using technology, people can control their health and take responsibility for health outcomes to a greater degree than is realistic or fair. Also, digital health empowerment often primarily reaches people who already have high technological and health literacy, leaving others behind. We critically investigate whether the concept of health empowerment could be re-engineered to mean something different, namely the process of improving the health literacy and access of those who least possess it, in line with recent proposals for conceptual engineering in the service of justice. We settle on the weaker conclusion that underlying ethical values including justice should be used to interpret the existing concept of empowerment. To conclude, we take a high-level view of various strategies for achieving the ethical value associated with digital health empowerment.
Impact of leadership communication on job satisfaction and well-being of physicians
Authors: Heuss Sabina C. , Datta Souvik 
Published: October 2023

Abstract
Background
Communication and leadership communication are key for an efficient performance of physicians. They hold a team together, help hospitals to develop and retain capable employees, and are directly related to physicians’ job satisfaction. We aim to test the impact of leadership models and various items of leadership communication on job satisfaction and well-being of physicians working in Swiss hospitals.

Method
We conducted a nationwide survey of physicians in Swiss hospitals in 2019 to assess the correlation between communication and leadership style with job satisfaction and well-being of physicians. The evaluation of the survey uses multivariate, statistical analysis (descriptive evaluations, regression model, factor analyses).

Results
The data reveal that leadership communication, e.g., the tone of communication, information quality and information behaviour and feedback have the most positive impact on job satisfaction and well-being. These items have a stronger influence on well-being when physicians belong to a younger generation, such as Generation Y (born after 1980). Leadership communication has the greatest impact on job satisfaction on residents and a lesser impact on physicians in higher hierarchical levels.

Conclusions
To increase job satisfaction and well-being amongst physicians, hospitals superiors and supervisors are advised to invest in education and training on leadership communication and feedback with special focus on the younger generation as well as on female physicians as this will have the greatest impact.
Cybernetic Aesthetics
Authors: Love Heather A. 
Published: October 2023

Cybernetic Aesthetics draws from cybernetics theory and terminology to interpret the communication structures and reading strategies that modernist text cultivate. In doing so, Heather A. Love shows how cybernetic approaches to communication emerged long before World War II; they flourished in the literature of modernism's most innovative authors. This book engages a range of literary authors, including Ezra Pound, John Dos Passos, Gertrude Stein, Virginia Woolf, and James Joyce, and cybernetics theorists, such as Norbert Wiener, Claude Shannon, Ross Ashby, Silvan Tomkins, Margaret Mead, Gregory Bateson, and Mary Catherine Bateson. Through comparative analysis, Love uncovers cybernetics' relevance to modernism and articulates modernism's role in shaping the cultural conditions that produced not merely technological cybernetics, but also the more diffuse notion of cybernetic thinking that still exerts its influence today.
Regarding the Montreal Protocol communication after the Kigali Amendment
Authors: Conde Júlio J. , Meira-Cartea Pablo Á. 
Published: October 2023

Abstract
The Kigali Amendment introduced a new family of chemical compounds, which do not contribute to stratospheric ozone depletion but present a high global warming potential, under the watch of the Montreal Protocol in 2016. Earlier this year, a press note from the World Meteorological Organization entitled “Ozone layer recovery is on track, helping avoid global warming by 0.5°C” caught our attention because of the wrong conclusions that can be potentially drawn by laypersons due to an apparent linkage of ozone depletion and global warming problems. Public communication of the Montreal Protocol since the Kigali Amendment should be more careful than ever to avoid lessening the social perception of the threat of climate change, particularly considering that society already has a distorted representation of these problems, assuming causal relations between ozone depletion and climate change, that could lead to unfounded optimism towards the climate crisis.
Sclerostin antibody improves alveolar bone quality in the Hyp mouse model of X-linked hypophosphatemia (XLH)
Authors: Carpenter Kelsey A. , Alkhatib Delia O. , Dulion Bryan A. , Guirado Elizabeth , Patel Shreya , Chen Yinghua , George Anne , Ross Ryan D. 
Published: October 2023

AbstractX-linked hypophosphatemia (XLH) is a rare disease of elevated fibroblast growth factor 23 (FGF23) production that leads to hypophosphatemia and impaired mineralization of bone and teeth. The clinical manifestations of XLH include a high prevalence of dental abscesses and periodontal disease, likely driven by poorly formed structures of the dentoalveolar complex, including the alveolar bone, cementum, dentin, and periodontal ligament. Our previous studies have demonstrated that sclerostin antibody (Scl-Ab) treatment improves phosphate homeostasis, and increases long bone mass, strength, and mineralization in the Hyp mouse model of XLH. In the current study, we investigated whether Scl-Ab impacts the dentoalveolar structures of Hyp mice. Male and female wild-type and Hyp littermates were injected with 25 mg·kg−1 of vehicle or Scl-Ab twice weekly beginning at 12 weeks of age and euthanized at 20 weeks of age. Scl-Ab increased alveolar bone mass in both male and female mice and alveolar tissue mineral density in the male mice. The positive effects of Scl-Ab were consistent with an increase in the fraction of active (nonphosphorylated) β-catenin, dentin matrix protein 1 (DMP1) and osteopontin stained alveolar osteocytes. Scl-Ab had no effect on the mass and mineralization of dentin, enamel, acellular or cellular cementum. There was a nonsignificant trend toward increased periodontal ligament (PDL) attachment fraction within the Hyp mice. Additional PDL fiber structural parameters were not affected by Scl-Ab. The current study demonstrates that Scl-Ab can improve alveolar bone in adult Hyp mice.
Calbindin 2-specific deletion of arginase 2 preserves visual function after optic nerve crush
Authors: Zaidi Syed A. H. , Xu Zhimin , Lemtalsi Tahira , Sandow Porsche , Athota Sruthi , Liu Fang , Haigh Stephen , Huo Yuqing , Narayanan S. Priya , Fulton David J. R. , Rojas Modesto A. , Fouda Abdelrahman Y. , Caldwell Robert W. , Caldwell Ruth B. 
Published: October 2023

AbstractWe previously found that global deletion of the mitochondrial enzyme arginase 2 (A2) limits optic nerve crush (ONC)-induced neuronal death. Herein, we examined the cell-specific role of A2 in this pathology by studies using wild type (WT), neuronal-specific calbindin 2 A2 KO (Calb2cre/+ A2 f/f), myeloid-specific A2 KO (LysMcre/+ A2f/f), endothelial-specific A2 KO (Cdh5cre/+ A2f/f), and floxed controls. We also examined the impact of A2 overexpression on mitochondrial function in retinal neuronal R28 cells. Immunolabeling showed increased A2 expression in ganglion cell layer (GCL) neurons of WT mice within 6 h-post injury and inner retinal neurons after 7 days. Calb2 A2 KO mice showed improved neuronal survival, decreased TUNEL-positive neurons, and improved retinal function compared to floxed littermates. Neuronal loss was unchanged by A2 deletion in myeloid or endothelial cells. We also found increased expression of neurotrophins (BDNF, FGF2) and improved survival signaling (pAKT, pERK1/2) in Calb2 A2 KO retinas within 24-hour post-ONC along with suppression of inflammatory mediators (IL1β, TNFα, IL6, and iNOS) and apoptotic markers (cleavage of caspase3 and PARP). ONC increased GFAP and Iba1 immunostaining in floxed controls, and Calb2 A2 KO dampened this effect. Overexpression of A2 in R28 cells increased Drp1 expression, and decreased mitochondrial respiration, whereas ABH-induced inhibition of A2 decreased Drp1 expression and improved mitochondrial respiration. Finally, A2 overexpression or excitotoxic treatment with glutamate significantly impaired mitochondrial function in R28 cells as shown by significant reductions in basal respiration, maximal respiration, and ATP production. Further, glutamate treatment of A2 overexpressing cells did not induce further deterioration in their mitochondrial function, indicating that A2 overexpression or glutamate insult induce comparable alterations in mitochondrial function. Our data indicate that neuronal A2 expression is neurotoxic after injury, and A2 deletion in Calb2 expressing neurons limits ONC-induced retinal neurodegeneration and improves visual function.
The mechanism of linear ubiquitination in regulating cell death and correlative diseases
Authors: Gao Liyuan , Zhang Wei , Shi Xiao hui , Chang Xiaoyan , Han Yi , Liu Chundi , Jiang Zhitao , Yang Xiang 
Published: October 2023

AbstractLinear ubiquitination is a specific post-translational modification in which ubiquitin is linked through M1 residue to form multiple types of polyubiquitin chains on substrates in order to regulate cellular processes. LUBAC comprised by HOIP, HOIL-1L, and SHARPIN as a sole E3 ligase catalyzes the generation of linear ubiquitin chains, and it is simultaneously adjusted by deubiquitinases such as OTULIN and CYLD. Several studies have shown that gene mutation of linear ubiquitination in mice accompanied by different modalities of cell death would develop relative diseases. Cell death is a fundamental physiological process and responsible for embryonic development, organ maintenance, and immunity response. Therefore, it is worth speculating that linear ubiquitin mediated signaling pathway would participate in different diseases. The relative literature search was done from core collection of electronic databases such as Web of Science, PubMed, and Google Scholar using keywords about main regulators of linear ubiquitination pathway. Here, we summarize the regulatory mechanism of linear ubiquitination on cellular signaling pathway in cells with apoptosis, necroptosis, autophagy, pyroptosis, and ferroptosis. Intervening generation of linear ubiquitin chains in relative signaling pathway to regulate cell death might provide novel therapeutic insights for various human diseases.
Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect
Authors: Chu Yu-De , Cheng Li-Chun , Lim Siew-Na , Lai Ming-Wei , Yeh Chau-Ting , Lin Wey-Ran 
Published: October 2023

AbstractColorectal cancer (CRC) is a prevalent malignancy worldwide and is associated with a high mortality rate. Changes in bioenergy metabolism, such as the Warburg effect, are often observed in CRC. Aldolase B (ALDOB) has been identified as a potential regulator of these changes, but its exact role in CRC cell behavior and bioenergetic homeostasis is not fully understood. To investigate this, two cohorts of CRC patients were analyzed independently. The results showed that higher ALDOB expression was linked to unfavorable prognosis, increased circulating carcinoembryonic antigen (CEA) levels, and altered bioenergetics in CRC. Further analysis using cell-based assays demonstrated that ALDOB promoted cell proliferation, chemoresistance, and increased expression of CEA in CRC cells. The activation of pyruvate dehydrogenase kinase-1 (PDK1) by ALDOB-induced lactagenesis and secretion, which in turn mediated the effects on CEA expression. Secreted lactate was found to enhance lactate dehydrogenase B (LDHB) expression in adjacent cells and to be a crucial modulator of ALDOB-mediated phenotypes. Additionally, the effect of ALDOB on CEA expression was downstream of the bioenergetic changes mediated by secreted lactate. The study also identified CEA cell adhesion molecule-6 (CEACAM6) as a downstream effector of ALDOB that controlled CRC cell proliferation and chemoresistance. Notably, CEACAM6 activation was shown to enhance protein stability through lysine lactylation, downstream of ALDOB-mediated lactagenesis. The ALDOB/PDK1/lactate/CEACAM6 axis plays an essential role in CRC cell behavior and bioenergetic homeostasis, providing new insights into the involvement of CEACAM6 in CRC and the Warburg effect. These findings may lead to the development of new treatment strategies for CRC patients.
Downregulation of EphA2 stability by RNF5 limits its tumor-suppressive function in HER2-negative breast cancers
Authors: Li Xiaojuan , Wang Fan , Huang Lu , Yang Mengtian , Kuang Ersheng 
Published: October 2023

AbstractEphrin receptor A2 (EphA2) plays dual functions in tumorigenesis through ligand-independent tumor promotion or ligand-dependent tumor suppression. However, the regulation of EphA2 tumor-suppressive function remains unclear. Here, we showed that RNF5 interacts with EphA2 and induces its ubiquitination and degradation, decreases the stability and cell surface distribution of EphA2 and alters the balance of its phosphorylation at S897 and Y772. In turn, RNF5 inhibition decreases ERK phosphorylation and increases p53 expression through an increase in the EphA2 level in HER2-negative breast cancer cells. Consequently, RNF5 inhibition increases the adhesion and decreases the migration of HER2-negative breast cancer cells, and RNF5 silencing suppresses the growth of xenograft tumors derived from ER-positive, HER2-negative breast cancer cells with increased EphA2 expression and altered phosphorylation. RNF5 expression is inversely correlated with EphA2 expression in breast cancers, and a high EphA2 level accompanied by a low RNF5 level is related to better survival in patients with ER-positive, HER2-negative breast cancers. These studies revealed that RNF5 negatively regulates EphA2 properties and suppresses its tumor-suppressive function in HER2-negative breast cancers.
Inhibition of mitochondrial fission activates glycogen synthesis to support cell survival in colon cancer
Authors: Hasani Sumati , Young Lyndsay E. A. , Van Nort Warren , Banerjee Moumita , Rivas Dylan R. , Kim Jinhwan , Xiong Xiaopeng , Sun Ramon C. , Gentry Matthew S. , Sesaki Hiromi , Gao Tianyan 
Published: October 2023

AbstractMetabolic reprogramming has been recognized as one of the major mechanisms that fuel tumor initiation and progression. Our previous studies demonstrate that activation of Drp1 promotes fatty acid oxidation and downstream Wnt signaling. Here we investigate the role of Drp1 in regulating glycogen metabolism in colon cancer. Knockdown of Drp1 decreases mitochondrial respiration without increasing glycolysis. Analysis of cellular metabolites reveals that the levels of glucose-6-phosphate, a precursor for glycogenesis, are significantly elevated whereas pyruvate and other TCA cycle metabolites remain unchanged in Drp1 knockdown cells. Additionally, silencing Drp1 activates AMPK to stimulate the expression glycogen synthase 1 (GYS1) mRNA and promote glycogen storage. Using 3D organoids from Apcf/f/Villin-CreERT2 models, we show that glycogen levels are elevated in tumor organoids upon genetic deletion of Drp1. Similarly, increased GYS1 expression and glycogen accumulation are detected in xenograft tumors derived from Drp1 knockdown colon cancer cells. Functionally, increased glycogen storage provides survival advantage to Drp1 knockdown cells. Co-targeting glycogen phosphorylase-mediated glycogenolysis sensitizes Drp1 knockdown cells to chemotherapy drug treatment. Taken together, our results suggest that Drp1-loss activates glucose uptake and glycogenesis as compensative metabolic pathways to promote cell survival. Combined inhibition of glycogen metabolism may enhance the efficacy of chemotherapeutic agents for colon cancer treatment.
Novel intrathoracic irrigation using ultrafine ozone bubbles in a rat empyema model
Authors: Ikeda Masaki , Yutaka Yojiro , Chen-Yoshikawa Toyofumi F. , Tanaka Michio , Yamamoto Masaki , Tanaka Satona , Yamada Yoshito , Ohsumi Akihiro , Nakajima Daisuke , Hamaji Masatsugu , Yoshizawa Akihiko , Kusaka Eishi , Nagao Miki , Date Hiroshi 
Published: October 2023

AbstractDissolved ozone is generally used for sanitization, but it has not been used for thoracic cavity sanitization because of its short half-life (< 20 min) and possible toxicity. We developed a novel solution containing ultrafine ozone bubbles (ozone-UFB) with a fivefold longer half-life than non-UFB ozone. Using an in vitro model, Staphylococcus aureus colonies were counted after exposure to ozone-UFB or non-UFB ozone at the same ozone concentration (0.4 mg/L). The colony count was significantly lower in the ozone-UFB group than in the non-UFB ozone group (p = 0.034). The effect of repeated pleural irrigation using ozone-UFB and saline was compared in a rat empyema model of S. aureus infection. The bacterial count in the pleural effusion was decreased by at least fivefold following intrathoracic lavage with ozone-UFB (3 mg/L). To examine the safety of ozone-UFB for intrathoracic use, ozone-UFB with a higher ozone concentration (10 mg/L) was injected into the thoracic cavities of normal rats. The treatment did not result in any specific pleural damage or elevated serum interleukin-6 concentrations. The findings highlighted the efficacy and safety of ozone-UFB for intrathoracic sanitization, but further studies are needed to determine the optimal therapeutic ozone concentration with appropriate safety margins.