bluuebunny/crossref_metadata_2025_split huggingface dataset:
IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction
Authors: Li Dexin , Cui Xinyu , Li Yingchao , Zhang Qin , Gao Hongyan , Li Youbo , Hou Yanmeng , Yuan Hongjie , Xiao Yihong 
Published: January 2025

Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most harmful pathogens in the swine industry. Our previous studies demonstrated that the small extracellular domain (ECL2) of CLDN4 effectively blocks PRRSV infection. In this study, we explored the in vivo administration of swine ECL2 (sECL2) and found that it blocked HP-PRRSV infection and alleviated histopathological changes in organs. Notably, sECL2 stimulated cytokine production in the lungs. We observed that CLDN4 upregulated the expression of IFN-β at low doses of GP3. While high doses of GP3 inhibited the activity of the IFN-β promotor, regardless of whether CLDN4 was present. GP3 also downregulated IFN-β by decreasing the phosphorylation of TBK1 and IRF3. These findings highlight functional differences in GP3 under quantity control, which account for the variations in IFN-β induction during the early and late stages of infection. Our results indicate that sECL2 is a promising candidate drug for developing treatments to control PRRS.
Navigating secure storage requirements for EUDI Wallets: a review paper
Authors: Ebadi Ansaroudi Zahra , Sciarretta Giada , De Maria Andrea , Ranise Silvio 
Published: January 2025

Abstract
The European Digital Identity Wallet (EUDI Wallet) plays a pivotal role in shaping digital identity across the EU, necessitating a secure storage solution compliant with eIDAS2 regulation. In this study, we first analyze mobile secure storage solutions for the EUDI Wallet, identifying any gaps between current offerings and the EUDI Wallet’s requirements. Our analysis indicates a significant shortfall in the availability of mobile native solutions, with only approximately 10.5% of mobile phones in the Italian market currently adhering to the stringent requirements mandated by eIDAS2. Consequently, we explore and evaluate alternative design solutions in terms of their feasibility and integration potential.
The impact of social distancing on mental health during the COVID-19 pandemic: a nationwide study of 4.6 million Danish adults
Authors: Geest Andreas , Bonnesen Barbara , Jordan Alexander , Tønnesen Louise , Rømer Valdemar , Ulrik Charlotte S. , Harboe Zitta , Eklöf Josefin , Sivapalan Pradeesh , Jensen Jens-Ulrik Stæhr 
Published: January 2025

Abstract

Background
Current knowledge on psychiatric illness following periods of social distancing during the COVID-19 pandemic is mostly limited to smaller studies in selected populations. This nationwide study of all 4.6 million Danish adults examined if periods of social distancing were associated with changes in surrogate measures of mental health.

Methods
All Danish adults (≥18 years) were included and rates of collection of antidepressant prescriptions, psychiatric hospital admissions, and suicide or suicide attempts for the periods March 12, 2020–May 20, 2020 (lockdown period 1), and December 21, 2020–March 1, 2021 (lockdown period 2), were compared to corresponding periods 1 year prior. Individuals were censored due to death or SARS-CoV-2 infection.

Results
Antidepressant consumption increased for both period 1 and period 2, with an incidence rate ratio (IRR) of 1.02 (95% CI: 1.01–1.02, p < 0.001) and IRR 1.08 (95% CI: 1.08–1.09, p < 0.001) respectively, compared to the control periods. Psychiatric hospitalization rates decreased significantly, with an IRR of 0.65 (95% CI: 0.63–0.66, p < 0.001) for period 1, and IRR 0.86 (95% CI: 0.84–0.88, p < 0.001) for period 2. The risk of suicide did not increase in period 1, IRR 0.96 (95% CI: 0.82–1.13, p = 0.64), but seemed increased during period 2, IRR 1.19 (95% CI: 1.02–1.38, p = 0.03).

Conclusion
Periods of social distancing during the COVID-19 pandemic were associated with an increase of antidepressant consumption, but decreased rates of psychiatric hospitalization. Suicide risk seemed increased during the second lockdown period.
Searching for Monogenic Autoimmune Etiology in Patients With Type 1 Diabetes Onset Before 30 Months of Age
Authors: Saso-Jiménez Laura , Urrutia Inés , Calvo Begona , Bilbao José Ramón , Gómez-Gila Ana Lucía , Leiva-Gea Isabel , Jiménez-Sanchis Andrea , Rica Itxaso , Castano Luis , Martínez Rosa 
Published: January 2025

Abstract

Introduction
The most frequent form of diabetes in pediatric patients is polygenic autoimmune diabetes (type 1 diabetes [T1D]), but single-gene variants responsible for autoimmune diabetes have also been described. Both disorders share clinical features, which can lead to monogenic forms being misdiagnosed as T1D. However, correct diagnosis is crucial for therapeutic choice, prognosis, and genetic counseling. The aim of this study was to search for monogenic autoimmune diabetes in Spanish pediatric patients with early-onset T1D.

Methods
Among 500 Spanish pediatric patients with T1D, those with disease onset between 9 and 30 months of age were selected for screening for monogenic autoimmune diabetes (n = 44). Genetic testing was performed by next-generation sequencing with a customized panel that included the major causative genes for monogenic autoimmune syndromes, including early-onset diabetes: AIRE, CTLA4, FOXP3, IL2RA, ITCH, LRBA, STAT1, STAT3, STAT5B. RT-PCR and cDNA sequencing of the RNA isolated from whole blood were used to analyze splicing variants.

Results
Genetic screening identified, in 2 patients with diabetes onset before 1 year of age, 2 likely pathogenic novel variants affecting canonical splicing sites: c.286-12_290del in STAT5B and c.-22-2delA in FOXP3. RNA analyses demonstrated that both variants modify mRNA splicing. The variant in STAT5B induced exon 4 skipping and the variant in FOXP3 caused a deletion of 16 nucleotides before the transcription start site.

Conclusion
T1D onset in the first year of life may indicate monogenic autoimmune diabetes and molecular testing may be recommended.
Revenue Sharing at Music Streaming Platforms
Authors: Bergantiños Gustavo , Moreno-Ternero Juan D. 
Published: January 2025

We study the problem of sharing the revenues raised from subscriptions to music streaming platforms among content providers. We provide direct, axiomatic, and game-theoretical foundations for two focal (and somewhat polar) methods widely used in practice: pro rata and user centric. The former rewards artists proportionally to their number of total streams. With the latter, each user’s subscription fee is proportionally divided among the artists streamed by that user. We also provide foundations for two families of methods addressing the rising concern in the music industry to explore new streaming models that better align the interests of artists, fans, and streaming services. One of the families offers a natural compromise between the pro rata and user-centric methods. The other family generalizes the user-centric method while capturing various formalizations of incentives for artists and users. This paper was accepted by Manel Baucells, behavioral economics and decision analysis. Funding: This work was supported by Xunta de Galicia [Grant ED431B2022/03] and MCIU/AEI/10.13039/501100011033 and FSE+ [Grants PID2020-113440GBI00, PID2020-115011GB-I00, and PID2023-146364NB-I00].
Linguagem Simples: um movimento social por transparência, cidadania e acessibilidade
Authors: Martins Heloísa Tavares , Da Silva Adriano Rosa , Teixeira Cavalcanti Márcia 
Published: January 2025

O presente trabalho se configura como uma revisão de literatura realizada com o objetivo de conceituar o movimento Linguagem Simples e expor um panorama da sua evolução no mundo, desde sua origem nos países de língua inglesa (Estados Unidos e Reino Unido), passando por iniciativas existentes na Europa e América Latina. Traz exemplos de como a Linguagem Simples vem sendo adotada por órgãos públicos no Brasil como uma ferramenta facilitadora da comunicação entre a Administração Pública e o cidadão. A partir desse cenário, narra como, ao longo dos anos, iniciativas isoladas deram origem a associações, criadas em vários países para a divulgação dos preceitos e diretrizes da Linguagem Simples, e que, em um esforço conjunto de três dessas associações, uma norma foi submetida à Organização Internacional de Normalização - ISO, com o intuito de padronizar as diretrizes da Linguagem Simples, de forma a atender à dos documentos, na maioria dos idiomas. O artigo também demonstra como a Linguagem Simples se relaciona com os conceitos de cidadania, transparência, inteligibilidade, legibilidade, letramento e acessibilidade textual e digital.Palavras-chave: linguagem simples; transparência; acessibilidade; cidadania.
A Petrobras e o Estado do Rio de Janeiro na rota da transição energética global
Authors: Guerra Wander 
Published: January 2025

As mudanças climáticas tomaram conta do debate mundial nos últimos anos, e com isso, a transição energética ganhou relevância na agenda de empresas, governos e instituições. O Rio de Janeiro, sendo o estado brasileiro que mais produz petróleo, encontra-se na rota das estratégias que atingem diretamente a indústria petrolifera, principalmente, a partir da lógica da descarbonização e pelos imperativos do capitalismo verde. Os efeitos da transição energética com vistas à redução da queima de combustíveis fósseis, podem ser diversos à depender da forma que o processo transitório será conduzido. Este artigo, a partir de uma revisão bibliográfica e de pesquisa exploratória, busca apresentar possíveis impactos da transição energética global na economia fluminense, tendo como foco a atuação da Petróleo Brasileira S.A, maior estatal brasileira que tem concentrado ações e investimentos na região sudeste do Brasil. Veremos que a transição energética capitaneada pelo capitalismo verde, principalmente a partir das energias dita renováveis podem trazer impactos negativos para a sociedade fluminense, ao passo que poderá perpetuar possíveis sacrifícios ambientais da indústria do petroleo. Ao mesmo tempo, ao deter uma ampla infraestrutura consolidada da indústria do petroleo, o Rio de Janeiro pode sair na vanguarda de uma transição energética justa no Brasil e a Petrobras tem papel central no processo.
Complexidade da indústria petrolífera na região metropolitana do Rio de Janeiro
Authors: Vasconcellos Bianca , Lebre La Rovere Renata , S. Pereira Rafael 
Published: January 2025

No estado do Rio de Janeiro, as principais dinâmicas econômicas sempre estiveram concentradas na Região Metropolitana (RMRJ). Há algum tempo o setor petrolífero tem se tornado um dos principais setores da economia regional. O presente trabalho busca investigar a complexidade industrial do setor petrolífero na RMRJ e seu impacto na estrutura produtiva regional. Para isso, foi aplicado a metodologia desenvolvida por Hausmann e Hidalgo sobre complexidade econômica. Observou-se que há predominância de indústrias de complexidade baixa e mediana. Os resultados mostram que há ausência /ou pouca participação de indústrias do setor petrolífero de alta complexidade. Isso implica na limitação da diversificação e do potencial desenvolvimento econômico da região. A análise das ligações entre as indústrias petrolíferas e outras atividades econômicas revela um "espaço vazio" na estrutura produtiva da RMRJ. Este trabalho se apresenta como mais uma peça de discussão visando a plena utilização da capacidade do Complexo Petroquímico. Bem como, insights para políticas que incentivem a diversificação visando o adensamento da cadeia produtiva e para impulsionar o crescimento econômico regional e, por consequência, de todo o estado
Lazhari Labter, Dictionnaire algérien illustré de la bande dessinée et du dessin de presse 1962-2022, Argel: CROM Éditions & Ministère de la Culture et des Arts, 2023, 480 pp. [ISBN: 978-9969-9728-0-1]
Authors: Hadj-Ali Mouhoub Souad 
Published: January 2025

Es una reseña del Dictionnaire algérien illustré de la bande dessinée et du dessin de presse 1962-2022, de Lazhari Labter, editado en Argel, CROM Éditions & Ministère de la Culture et des Arts,2023, 480 pp. [ISBN: 978-9969-9728-0-1]
Comparative analysis of inflammatory biomarkers for the diagnosis of neonatal sepsis: IL-6, IL-8, SAA, CRP, and PCT
Authors: Chen Ying , Yan Aixia , Zhang Li , Hu Xiaoming , Chen Liang , Cui Jun , Fan Zichuan , Li Ying 
Published: January 2025

Abstract
Neonatal sepsis (NS) is highly likely to cause death; however, early diagnosis of NS is still a great challenge. This study aimed to determine the diagnostic values of IL-6, IL-8, and serum amyloid A (SAA) in NS patients. C-Reactive protein (CRP), procalcitonin (PCT), interleukin (IL)-6, IL-8, and SAA were detected in 120 infants with NS (60 premature infants [NS-PIs] and 60 term infants [NS-TIs]). Sixty noninfected premature infants and 60 noninfected term infants composed the control group. Receiver operating characteristic (ROC) curves were used to determine the sensitivity and specificity of IL-6, IL-8, and SAA alone and in combination with CRP and PCT. The area under the curve (AUC) was calculated to evaluate the diagnostic value. The clinical characteristics of the subjects were recorded. The expression of CRP, PCT, IL-6, IL-8, and SAA was upregulated in patients with NS compared with control subjects. When the SAA cut-off value was 10.18 mg/L, the greatest AUC for the diagnosis of NS-PIs was for SAA (AUC = 0.833, 95% CI 0.762–0.905, P < 0.001). When the CRP cut-off value was 9.562 mg/L, the smallest AUC for the diagnosis of NS-PIs was for CRP (AUC = 0.776, 95% CI 0.684–0.867, P < 0.001). When the IL-8 cut-off value was 52.03 pg/mL, the greatest AUC for the diagnosis of NS-TIs was for IL-8 (0.821). When the IL-8 cut-off value was 52.03 pg/mL, the greatest AUC for the diagnosis of NS-TIs was for IL-8 (AUC = 0.821, 95% CI 0.745–0.898, P < 0.001). When the CRP cut-off value was 13.18 mg/L, the smallest AUC for the diagnosis of NS-TIs was for CRP (AUC = 0.762, 95% CI 0.667–0.857; P < 0.001). Additionally, according to the AUC value, the best combination was SAA and PCT for NS-PI diagnosis, and the best combination was PCT and IL-6 for NS-TI. In conclusion, compared with PCT and CRP, IL-6, IL-8, and SAA are better diagnostic biomarkers. Moreover, PCT combined with SAA is more suitable for diagnosing NS-PIs, and PCT combined with IL-6 is more suitable for diagnosing NS-TIs.
Post-pandemic insights on COVID-19 and premature ovarian insufficiency
Authors: Han Yaguang , Dai Yang , Wang Kexin , Zhang Xin , Shao Zishen , Zhu Xiaolin 
Published: January 2025

Abstract
The COVID-19 pandemic has raised concerns regarding its potential impact on premature ovarian insufficiency (POI). This overview examines the possible interactions between COVID-19 and POI, while also suggesting preventive measures. The viral infection’s inflammatory response and immune dysregulation may adversely affect ovarian tissues, leading to inflammation and damage. Additionally, alterations in vascular function could impair ovarian blood flow and hormonal imbalances may disrupt normal ovarian function. Long-term health effects, such as “long COVID,” may exacerbate these issues through chronic inflammation and immune dysfunction. Public health measures, such as vaccination and home isolation, may indirectly protect ovarian health by reducing systemic inflammation. Vaccines could mitigate the severity of COVID-19’s impact on ovarian function, while isolation may reduce stress and inflammation. However, further research is needed to validate these mechanisms.
Evaluation of AUTION EYE AI-4510 flow cell morphology analyzer for counting particles in urine
Authors: Oyaert Matthijs , Kouri Timo , Carton Eva , Deprez Sigrid , Lambrecht Stijn , Speeckaert Marijn 
Published: January 2025

Abstract

Objectives
We evaluated the performance of a novel flow cell morphology analyzer AUTION EYE AI-4510 for counting particles in urine.

Methods
Analytical performance was assessed according to the EFLM European Urinalysis Guideline 2023. Trueness was compared by analyzing 1.012 fresh urine samples with the AUTION EYE AI-4510 (ARKRAY, Inc., Kyoto, Japan) against phase-contrast visual microscopy. Poisson statistics were utilized in assessment of imprecision of particle counts both with quality control material and patient specimens.

Results
Relative imprecision against theoretical Poisson imprecision, R(CV), was estimated to be 1.1 for red blood cells (RBC), 1.0 for white blood cells (WBC), 0.9 for squamous epithelial cells (SEC) and 1.1 for bacteria. The agreement with visual microscopy (Cohen’s weighted kappa) was 0.93 for RBC, 0.95 for WBC, 0.90 for SEC, 0.79 for non-squamous epithelial cells (NSEC), 0.67 for combined casts, 0.90 for crystals and 0.88 for bacteria. No clinically significant bias was observed. Limits of quantitation at CV=30 % reached 4 × 106/L for RBC and 5 × 106/L for WBC. Differentiation of urinary crystals was improved as compared to previous data on digital cuvette imaging.

Conclusions
The ARKRAY AUTION EYE AI-4510 provided a desirable imprecision, met the criteria for linearity, LoQ and carry-over, and showed an optimum comparison to visual microscopy for RBC, WBC, SEC and crystals as defined in the EFLM European Urinalysis Guideline 2023. The identification of kidney damage is recommended to be improved by using user-defined review rules. Performance of bacteria counting needs to be confirmed against urine bacterial cultures.
Overview of dengue diagnostic limitations and potential strategies for improvement
Authors: Selvaraj Aruna Devi , Ramaian Santhaseela Anand , Tamilmani Elavarasan 
Published: January 2025

Abstract

Introduction
Dengue is a viral infection caused by any one of the four related dengue virus (DENV) serotypes, 1–4. DENV is a single-stranded RNA virus belonging to the genus Flavivirus. Dengue can cause a range of symptoms, from mild to severe life-threatening illness. Currently, treatment for DENV is limited to supportive care, with better outcomes achieved through early diagnosis. The WHO has suggested that dengue mortality can be reduced to nearly zero by implementing appropriate clinical management strategies, such as early laboratory diagnosis. This calls for diagnostic approaches that combine high sensitivity and specificity, while also being suitable for point-of-care testing (POCT) in remote locations with minimal staff training and low testing costs.

Content
In this paper, we outline the limitations of existing confirmatory dengue diagnostic methods, such as ELISA and RT-PCR, which are time-consuming, expensive, and require skilled personnel. We also highlight alternative strategies to overcome these challenges. Additionally, the paper emphasizes the growing clinical demand for diagnosing severe dengue to reduce the risk of death, which must be addressed by next-generation dengue diagnostic approaches.

Summary
We propose the adoption of alternative strategies, such as fluorescence immunoassay (FIA) and chemiluminescence immunoassay (CLIA), which have the potential to overcome the limitations of existing dengue diagnostic methods.

Outlook
Improvements in dengue diagnosis, with a specific focus on identifying severe dengue in POCT setting, can help achieve the goal of zero deaths from dengue.
Neutronic analysis of a lithium-cooled space nuclear reactor based on sliding reflector design
Authors: Wang Yue , Wei Bin , Wang Jincheng , Zhang Wenchao , Sun Jianchuang , Cai Weihua 
Published: January 2025

Abstract
The sliding reflector control system is utilized for core power level regulation through neutron leakage, offering a simpler structure in comparison to the control drum and control rod systems, thus minimizing the mass of the space reactor. This paper presents a conceptual design of a small – scale homogeneous lithium-cooled space fast reactor based on the sliding reflector control system. In this study, accurate modeling of the radial subregion of the reflector block is achieved, and the influence mechanism of the leakage rate on the key parameters of core neutronics is quantitatively analyzed. By pulling down the sliding reflector layer until the core reaches the critical state, the designed core shows a negative reactivity temperature coefficient of −0.3083 pcm/K. Meanwhile, the peak value of the inhomogeneity coefficients for axial and radial neutron flux density distributions are 1.414 and 1.268, respectively, satisfying safety and economic requirements. The analysis indicates that changes in the neutron leakage rate directly impact the reactivity. Building upon these findings, three reflector arrangement schemes summarizing all possible sliding scenarios are proposed: the uplift sliding reflector, the down-sliding reflector, and the intermediate separating sliding reflector. The results of neutron calculations show that the down-sliding reflector has the largest adjustment accuracy, with reaching criticality when the axial leakage length reaches 2.85 cm. The uplift reflector produces the most uniform axial neutron flux density distribution, peaking at 1.341.
Risk analysis and protection in case of intrusion of nuclear facilities
Authors: Wadoud Amir A. , Saleh Alaa A. , Abdel-Rahman Mohamed A.E. 
Published: January 2025

Abstract
Physical protection systems (PPSs) are designed to counter and neutralize any theft or sabotage attempts carried out against a given site. PPS designs are crucial, requiring rigorous analysis and evaluation to ensure their reliability, sustainability and optimal security. This paper assumes a Hypothetical Nuclear Research Center (HNRC) for PPS analysis and evaluation. This work will determine the PPS’s most vulnerable path elements, by demonstrating expected intrusion attack scenarios by taking advantage of the HNRC’s design weaknesses. Also, this work determines the threats resulting from adversary intrusion through the weakness path elements located at the nuclear site. Additionally, this paper develops an analytical methodology for this evaluation and risk assessment, by using the Systematic Analysis of Vulnerability to Intrusion (SAVI) computer program. Through constructing adversary sequence diagrams (ASDs), SAVI can easily identify the HNRC’s most vulnerable paths, pinpointing the areas which require the most attention. Alongside SAVI is the quantitative risk analysis method - the Dai et al. Model – which can calculate the effectiveness and risk of the PPS itself by measuring its degree of uncertainty using effectiveness entropy. To comply with effectiveness and risk standards, this work also introduces security system upgrade processes and determines the optimal security solution for the HNRC.