bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan e-modul pembelajaran IPA berorientasi etnosains alat musik pada materi gelombang bunyi untuk meningkatkan kemampuan berpikir kritis peserta didik
Authors: Riska Dara , Mayub Afrizal , Karyadi Bhakti 
Published: January 2025

Penelitian ini bertujuan untuk mengembangkan e-modul pembelajaran IPA berorientasi etnosains alat musik pada materi gelombang bunyi untuk meningkatkan kemampuan berpikir kritis peserta didik SMPN Kota Bengkulu. Metode penelitian yang digunakan adalah R&D dengan model ADDIE yaitu Analisis (analysis), Perancangan (design), Pengembangan (developmen), Implementasi (Implementation), dan Evaluasi (evaluation).Penelitian dilakukan pada 33 siswa kelas VIII SMPN 2 Kota Bengkulu dengan menggunakan metode one group pretest-posttest design. Hasil penelitian menunjukkan bahwa: 1) Siswa menyatakan bahwa perlu dikembangkannya modul elektronik (e-modul) etnosains untuk meningkatkan kemampuan berpikir kritis pada pembelajaran IPA. 2) Hasil validasi ahli dan praktisi instrumen kelayakan e-modul yang terdiri dari empat aspek, yaitu aspek kegrafisan, aspek media, aspek kelayakan Bahasa, aspek kelayakan penyajian, dan aspek kelayakan isi dinyatakan layak dengan rata-rata persentase sebesar 87.20 % 3) Hasil validasi ahli dan praktisi instrumen kemampuan berpikir kritis yang terdiri dari dimensi penjelasan sederhana (elementary clarification), membangun keterampilan dasar (basic support), membuat kesimpulan (inference), memberikan penjelasan lebih lanjut (advance clarification), dan mengatur strategi dan taktik (strategies and tactic) ) dinyatakan layak dengan ratarata persentase sebesar 93.33%. 4) Terdapat peningkatan kemampuan berpikir kritis peserta didik ssetelah menggunakan e-modul dengan kategori sedang dan diperoleh nilai signifikansi sebesar < 0.05, artinya ada pengaruh yang signifikan dengan penggunaan e-modul etnosains gelombang bunyi dalam meningkatkan kemampuan berpikir kritis peserta didik.5) Respon peserta didik terhadap e-modul etnosains gelombang bunyi yang dikembangkan dalam proses pembelajaran IPA mencapai persentase sebesar 90.79%. dengan kategori sangat baik.
Machine learning-based screening of asthma biomarkers and related immune infiltration
Authors: Zhong Xiaoying , Song Jingjing , Lei Changyu , Wang Xiaoming , Wang Yufei , Yu Jiahui , Dai Wei , Xu Xinyi , Fan Junwen , Xia Xiaodong , Zhang Weixi 
Published: January 2025

IntroductionAsthma has an annual increasing morbidity rate and imposes a heavy social burden on public healthcare systems. The aim of this study was to use machine learning to identify asthma-specific genes for the prediction and diagnosis of asthma.MethodsDifferentially expressed genes (DEGs) related to asthma were identified by examining public sequencing data from the Gene Expression Omnibus, coupled with the support vector machine recursive feature elimination and least absolute shrinkage and selection operator regression model. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene set enrichment analysis and correlation analyses between gene and immune cell levels were performed. An ovalbumin-induced asthma mouse model was established, and eukaryotic reference transcriptome high-throughput sequencing was performed to identify genes expressed in mouse lung tissues.ResultsThirteen specific asthma genes were obtained from our dataset analysis (LOC100132287, CEACAM5, PRR4, CPA3, POSTN, LYPD2, TCN1, SCGB3A1, NOS2, CLCA1, TPSAB1, CST1, and C7orf26). The GO analysis demonstrated that DEGs linked to asthma were primarily related to positive regulation of guanylate cyclase activity, gpi anchor binding, peptidase activity and arginine binding. The renin-angiotensin system, arginine biosynthesis and arginine and proline metabolism were the key KEGG pathways of DEGs. Additionally, the genes CEACAM5, PRR4, CPA3, POSTN, CLCA1, and CST1 expression levels were positively associated with plasma cells and resting mast cells. The mouse model revealed elevated nos2 and clca1 expression in the asthmatic mouse group compared with that in normal mice, which was consistent with the findings in asthmatic patients.DiscussionThis study identified new marker genes for the prediction and diagnosis of asthma, which can be further validated and applied clinically
Risk factors associated with pregnancy loss after single euploid blastocysts transfer
Authors: Dong Xiaohui , Shi Juanzi , Liu Xiaohua , Liu Danmeng , Li Wei , Zhao Xiaoli , Xue Xia 
Published: January 2025

ObjectiveTo detect primary factors influencing pregnancy loss(PL) following the transfer of euploid blastocysts?DesignRetrospective cohort study.MethodsTo identify potential factors influencing PL, we analyzed multiple variables in both the PL group and the live birth group. In order to minimize the impact of confounding factors, various variables were included in a binary logistic regression analysis.ResultsThe PL rate after the transfer of euploid embryos is 13.3% (36 cases of PL out of 270 cases of single embryo transfer). Compared to the live birth group, the PL group had lower E2 levels (2376.03 ± 1553.25 vs 3412.88 ± 2116.47, P=0.007), higher LH levels (2.66 ± 2.47 vs 1.96 ± 1.52, P=0.023) on trigger day of fresh cycle, fewer retrieved oocytes (10.08 ± 5.01 vs 12.77 ± 7.20, P=0.044), higher female BMI (23.26 ± 3.27 vs 22.05 ± 2.69, P=0.016), higher proportion of maternal smokers (50% vs 20.51%, P&lt;0.001), and more day 6 blastocyst transfers ((38.89% vs14.53, P=0.001). Logistic regression analysis revealed that higher LH levels(OR=1.304, 95%CI=1.054-1.613, P=0.015), low E2 levels (OR=0.438, 95%CI=0.242-0.794, P=0.007) on trigger day of fresh cycle, maternal smoking(OR=4.574, 95%CI=1.974-10.601, P&lt;0.001), and day 6 blastocyst transfer(OR=4.610, 95%CI=1.907-11.141, P=0.001) appeared to be associated with increased risk of PL following the transfer of euploid embryos.ConclusionsMaternal smoking, day of blastocyst transferred, estradiol (E2) and Luteinizing hormone (LH) levels on trigger day of corresponding fresh cycle for transferred blastocysts are all associated with PL following transfer of euploid embryos.
Risk factors and clinical outcomes of cytomegalovirus infection following haploidentical hematopoietic stem cell transplantation in patients with aplastic anemia: a single-center retrospective study
Authors: Feng Jia , Zhang Xinhe , Tan Zhengwei , Zhao Yuechao , Hu Huijin , Chen Junfa , Wu Liqiang , Yu Qinghong , Wu Dijiong , Ye Baodong , Liu Wenbin 
Published: January 2025

BackgroundCytomegalovirus (CMV) infection remains a critical cause of mortality after allogeneic hematopoietic stem cell transplantation, despite significant advancements in CMV prevention and treatment with the introduction and widespread use of letermovir. However, in China, due to limitations in the availability and cost of medications, some patients still face challenges in accessing letermovir. For this subset of the population, exploring the risk factors for CMV infection remains significant in predicting its occurrence.MethodsTherefore, a retrospective analysis was conducted on 88 haploidentical hematopoietic stem cell transplant recipients over 4 years.ResultsOur study results indicate that chronic graft-versus-host disease (cGVHD) is an independent risk factor for CMV infection following haploidentical hematopoietic stem cell transplantation (Haplo-HSCT). Survival analysis reveals lower survival rates in the refractory CMV infection (RCI) group compared to the non-RCI group, with patients having lower viral loads demonstrating higher rates of seroconversion and improved survival under the same treatment regimen.ConclusionStrengthening the monitoring of CMV-DNA in post-transplant patients, actively promoting hematopoietic recovery, preventing the occurrence of CMV infection, and controlling the development of CMV infection can lead to better survival outcomes for patients with aplastic anemia undergoing Haplo-HSCT.
Effect of afzelin on inflammation and lipogenesis in particulate matter-stimulated C. acnes-treated SZ95 sebocytes
Authors: Hong Ji Yeon , Choi Yong Hee , Roh Yoon Jin , Lee Mi-Kyung , Zouboulis Christos C. , Park Kui Young 
Published: January 2025

BackgroundAfzelin, a flavonoid (kaempferol 3-O-α-L-rhamnopyranoside) isolated from Thesium chinense, is known for its potent anti-inflammatory properties. However, its effects on the molecular aspects of inflammation and lipogenesis in SZ95 sebocytes has not been investigated.ObjectiveThis study aimed to (i) investigate inflammatory and sebum secretion changes when a Cutibacterium acnes-treated immortalized human sebocyte cell line (SZ95) is exposed to particulate matter (PM) and (ii) examine the effects of afzelin on these.MethodsTo investigate the effect of afzelin on PM- and C. acnes-treated SZ95 sebocytes, we injected heat-killed C. acnes into SZ95 cells to induce acne-like status. Thereafter, the SZ95 sebocytes were treated with PM and subsequently with afzelin. The gene expression profile was determined using real-time polymerase chain reaction analysis, and protein expression was confirmed via western blotting and immunofluorescence. Intracellular lipid droplet formation was investigated using Nile Red O staining.ResultsPM treatment upregulated the mRNA and protein expression levels of inflammatory cytokine and lipogenic genes in C. acnes-treated SZ95 sebocytes. Furthermore, intracellular lipid-droplet accumulation increased when C. acnes-stimulated SZ95 cells were exposed to PM. Interestingly, the upregulated inflammatory and lipogenic gene expression induced by C. acnes and PM was attenuated by afzelin treatment.ConclusionsThis study's findings indicate that PM potentially aggravates acne by acting on both inflammation and sebum secretion. They also reveal afzelin's ability to suppress these phenomena by not only suppressing inflammatory cytokine expression but also inhibiting sebogenesis. These findings confirm afzelin's potential therapeutic role in improving PM-exacerbated acne.
Predictive value of the systemic immune–inflammation index for outcomes in large artery occlusion treated with mechanical thrombectomy—a single-center study
Authors: Qian Ao , Zheng Longyi , He Hui , Duan Jia , Tang Shuang , Xing Wenli 
Published: January 2025

BackgroundThe systemic immune–inflammation index (SII) is a composite and easily available inflammation index, which can quantitatively reflect the degree of inflammation. This study aims to investigate the predictive value of admission SII for outcomes of large artery occlusion treated with mechanical thrombectomy (MT).MethodsThis retrospective study was conducted at Suining Central Hospital, Sichuan, China. Patients were stratified into quartiles based on their SII. The investigating outcomes included hemorrhagic transformation (HT), malignant brain edema (MBE), 90-day functional outcome, and mortality. The adverse function was defined as the modified Rankin Scale (mRS) score &gt; 2 at the 90-day follow-up. Multivariate analysis was performed to explore the relationships between SII and outcomes. In addition, cases (distinguished from the aforementioned patients) treated with MT + mild hypothermia (MH) were also included to elucidate the relationships between SII/MH and outcomes in a new cohort.ResultsA total of 323 patients treated with MT were included. The observed HT, MBE, adverse function, and mortality rates were 31.9, 25.7, 59.4, and 27.9%, respectively. Multivariate analysis demonstrated that heightened SII was significantly related to HT (odds ratio [OR]: 1.061, 95% confidence interval [CI]: 1.035–1.086, p &lt; 0.001), MBE (OR: 1.074, 95% CI: 1.045–1.103, p &lt; 0.001), adverse function (OR: 1.061, 95% CI: 1.031–1.092, p &lt; 0.001), and mortality (OR: 1.044, 95% CI: 1.018–1.070, p = 0.001), after adjusting sex, age, Glasgow Coma Scale (GCS) score at admission, initial National Institutes of Health Stroke Scale (NIHSS) score, baseline Alberta Stroke Program Early Computed Tomography Score (ASPECTS), present HMCAS, occluded vessel region, collateral score and successful revascularization. HT and MBE may partially account for patients with elevated SII’s adverse function and mortality. In addition, with the criterion of baseline ASPECTS ≤ 7, a total of 42 patients treated with MT + MH were enrolled to build up a new cohort combined with 72 patients treated with mere MT. The risk role of SII and protect effect of MH were identified for HT (SII—OR: 1.037, 95% CI: 1.001–1.074; MH—OR: 0.361, 95% CI: 0.136–0.957), MBE (SII—OR: 1.063, 95% CI: 1.019–1.109; MH—OR: 0.231, 95% CI: 0.081–0.653), and mortality (SII—OR: 1.048, 95% CI: 1.011–1.087; MH—OR: 0.343, 95% CI: 0.118–0.994).ConclusionElevated SII was related to HT, MBE, 90-day adverse function, and mortality after MT. The MH may improve prognosis under high inflammation status.
Standard b-value DWI-derived stiffness index analysis may provide a way to evaluate the development of intracerebral hematoma
Authors: Li Qian , Mao Jin , Wang Qiyuan , Yao Liding , Xu Fangfang , Dong Fei 
Published: January 2025

Background and purposeThe development of intracerebral hemorrhage (ICH) is closely related to mechanical forces. However, noninvasively evaluating mechanical forces for ICH patients in the current clinical setting is challenging. In this study, we aimed to build an easily accessible stiffness index (STI) and evaluate the stiffness of the perihematomal edema (PHE) region in ICH patients.Materials and methodsIn this retrospective study, two cohorts of 57 patients were included. One cohort (the exploratory cohort) comprised patients with both standard b-value diffusion-weighted imaging (sDWI) (b-values of 0 and 1,000 s/mm2, b0 and b1000) and higher b-value diffusion-weighted imaging (hDWI) (b-values of 200 and 1,500 s/mm2). Another cohort (the hemorrhage cohort) consisted of patients who were diagnosed with ICH and who underwent sDWI within 48 h from onset. The hDWI-based virtual shear modulus (μdiff) was calculated and correlated with the sDWI data in the exploratory cohort. In the hemorrhage cohort, STI maps that were used to estimate μdiff were generated. The mean STI (mSTI) and coefficient of variation (COV) of the STI were computed on the basis of the STI maps in the whole and largest-slice PHE regions.ResultsThe STI could be calculated with the Equation 0.047697*S1000-0.022944*S0 + 5.359883, where S1000 and S0 represent the signal intensities of the b1000 and b0 images, respectively. In the whole and largest-slice PHE regions, both the mSTI and COV were correlated with the hematoma volume (p &lt; 0.01), but neither were correlated with the time from onset.ConclusionThe standard b-value DWI-derived stiffness index analysis may provide a noninvasive and easily accessible way to evaluate the development of ICH.
‘We don’t know nearly enough’: an online survey exploring perspectives of specialists who support children with brain-based visual impairments
Authors: Jakubowski Josephine Sabrina , May Eloise , Findlay Rebecca , McDowell Nicola , Simkin Samantha K. , Hamm Lisa M. 
Published: January 2025

IntroductionChildren with brain-based visual impairments (some of whom have a diagnosis of Cerebral Visual Impairment, or ‘CVI’) represent a growing and underserved population within vision services. These children often have more complex needs than those with ocular visual impairments and benefit from specialist support from multiple disciplines. This study aimed to understand the perspectives of these specialists in terms of their goals, views on collaboration, and understanding of the term ‘CVI’.MethodsWe invited a range of specialists who work with children with brain-based visual impairments, including educators, rehabilitation staff, clinicians, and family members, to complete an online survey between April 2023 and April 2024.ResultsThe analysis included 94 respondents: 51 educators, 30 rehabilitation staff, 7 clinicians, and 6 family members. Respondents shared common goals of connecting with the child (87/94, 93%) and fostering their learning and development (82/94, 93%). However, respondents also noted some specific and divergent goals, which can be at odds with each other. Professional staff frequently identified family members as the most valuable source of information about their child’s vision (36/88, 41%), though family members expressed feeling under-valued. Transdisciplinary clinics were highlighted as a helpful model to provide quality child-centered care. Of the 73 professional staff who reported being familiar with the term ‘CVI’ (73/88, 83%), most (61/73, 84%) thought it was underdiagnosed, but respondents had different perspectives on what a diagnosis meant. Only 73% of professionals familiar with CVI reported receiving formal training about it.DiscussionThe varied goals and different perspectives on CVI create challenges to providing cohesive support for children with brain-based visual impairments. Increasing the availability of complementary formal training across disciplines and adopting transdisciplinary models of care are promising approaches to improve the quality of services.
Individual and combined effects of dietary vitamin intake on cognitive function in elderly adults: the potential mediating role of serum neurofilament light chain levels
Authors: Zhou Zhikui , Fan Baiyun , Chen Qiang , Li Xuezhong , Ke Xianjin 
Published: January 2025

BackgroundVitamins are essential micronutrients for the prevention and treatment of neurodegenerative diseases. The objectives of the present study were to evaluate the association between dietary vitamin intake and cognitive function in elderly adults and to explore the potential impact of serum neurofilament light chain (NfL) concentration.MethodsData from 468 elderly individuals, including information on the dietary consumption of 10 vitamins, were used. Cognitive performance was assessed according to a composite Z-score of the Animal Fluency Test (AFT), Consortium to Establish a Registry for Alzheimer’s Disease (CERAD), and Digit Symbol Substitution Test (DSST). Serum NfL levels were measured using a highly sensitive immunoassay. Bayesian kernel machine regression (BKMR) models were used to estimate the combined effects of vitamin mixtures on cognitive function.ResultsIn both single- and multiple-vitamin models, individuals with a higher intake of dietary vitamin K exhibited greater global cognitive function, compared to those with a lower vitamin intake. BKMR revealed positive associations between vitamin mixtures and global cognitive function, AFT Z-scores, and DSST Z-scores. Individuals in the third vitamin K intake tertile exhibited lower serum NfL levels than those in the first tertile (regression coefficient, β = −0.16 [95% confidence interval −0.29 to −0.02]; p = 0.023). Serum NfL levels mediated the association between higher vitamin K intake and global cognitive function (8.73%).ConclusionVitamin mixtures were positively associated with global cognitive function in elderly participants. The association between vitamin K intake and cognitive function may be mediated by serum NfL concentration.
Association between vitamin D levels and preserved ratio impaired spirometry: an investigation of mediating roles of systemic inflammation and metabolic indicators
Authors: Lin Tong , Huang Shanshan , Zhou Fen , Shen Xingkai , Mao Haiyan 
Published: January 2025

BackgroundPreserved ratio impaired spirometry (PRISm) represents an abnormal lung function state distinct from traditional chronic obstructive pulmonary disease, characterized by unique clinical and epidemiological features. PRISm has been associated with various health issues, including an increased risk of metabolic disorders and cardiovascular diseases. Vitamin D, known for its anti-inflammatory, immunomodulatory, and antioxidant properties, may play a role in reducing the risk of PRISm. This study aims to investigate the relationship between vitamin D levels and PRISm, including the mediating effects of systemic inflammation markers and metabolic indicators in a population of U.S. adults.MethodsThis cross-sectional study analyzed data from 17,333 participants from the U.S. National Health and Nutrition Examination Survey, including 1,577 individuals with PRISm and 15,756 without. Baseline characteristics were assessed, and multivariate logistic regression models were employed to examine the relationship between vitamin D and PRISm. Mediation analysis was conducted to explore potential mediating roles of systemic immune-inflammation index (SII), triglyceride-glucose (TyG) index, and bilirubin. Nonlinear relationships were assessed using restricted cubic spline (RCS) models.ResultsThe PRISm group had lower median vitamin D levels and distinct inflammatory and metabolic profiles compared to the non-PRISm group. Multivariate analysis confirmed an inverse association between vitamin D levels and PRISm (adjusted OR: 0.989, 95% CI: 0.984–0.994, p &lt; 0.001). RCS analysis showed a nonlinear protective effect of vitamin D, with risk stabilizing at levels above 50 nmol/mL. Mediation analysis highlighted bilirubin as a positive mediator (ACME = −4.11 × 10−5, p &lt; 0.001), while TyG demonstrated a suppressive mediation effect (ACME = 2.68 × 10−5, p &lt; 0.001). SII did not show significant mediation.ConclusionElevated vitamin D levels are linked to a lower risk of PRISm, with bilirubin potentially acting as a mediator in this protective relationship. This underscores the clinical significance of maintaining sufficient vitamin D levels to promote lung health and mitigate the prevalence of PRISm among U.S. adults. Further research is warranted to investigate personalized vitamin D supplementation strategies as a potential preventive approach.
Tumor-shrinking effects of enfortumab vedotin between primary urothelial carcinoma and metastatic organs
Authors: Ikarashi Daiki , Kawamura Tatsuya , Ogasawara Keita , Arakawa Yumeka , Machida Arisa , Ito Ayato , Shiomi Ei , Maekawa Shigekatsu , Kato Renpei , Kanehira Mitsugu , Sugimura Jun , Obara Wataru 
Published: January 2025

ObjectiveThis study aimed to determine and compare the tumor shrinkage rate and its durability by enfortumab vedotin treatment between primary urothelial carcinoma and metastatic organs.MethodsWe retrospectively evaluated the tumor shrinkage rate in 39 Japanese patients treated with enfortumab vedotin for advanced urothelial carcinoma. We also evaluated the periods of tumor shrinkage maintenance (the period when best response was maintained) and regrowth (the period from best response to tumor growth confirmation) between primary and metastatic organs.ResultsMeasurable metastatic organs included the lung in 17, lymph node in 22, liver in 6, and bone in 5 cases. Primary lesion was detected in 20 cases. The mean tumor shrinkage rates for lung, lymph node, liver, and bone metastases and primary sites were 21% (−212 to 100), 13% (−130 to 86), −8.5% (−158 to 85), −64% (−250 to 21), and 22% (−38 to 79), respectively. The tumor shrinkage was maintained for 5.9 (0.7–14) months in lung metastases, 8.3 (2.6–14.5) months in lymph node metastases, 3.6 months in liver metastases, 0.7 months in bone metastases, and 1.8 (0.7–5.4) months in primary sites, and the period of regrowth was 7.3 (2.2–19.4), 4.8 (2.0–8.9), 2.8, 6.5, and 2.5 (1.1–5.9) months, respectively.ConclusionsEnfortumab vedotin showed significant tumor shrinkage in the primary tumor, lung metastases, and lymph node metastases, whereas the durability of tumor shrinkage was limited in the primary tumor.