bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood culture isolates and antimicrobial sensitivities from 1621 critically ill neonatal foals (2005–2022)
Authors: Flood JA , Collins NM , Russell CM , Cuming RS , Carrick JB , Cudmore LA 
Published: January 2025

IntroductionSepsis is a leading cause of morbidity and mortality in neonatal foals. Administration of appropriate antimicrobials and early aggressive supportive treatment is central to the efficacious treatment of neonatal sepsis and has proven to positively influence outcomes. The primary aim of our study was to evaluate microorganism results and antimicrobial sensitivities of neonatal foals with a positive blood culture submitted to our intensive care unit (ICU) during 2005–2022. The secondary aim was to compare the results to those of a similar previous study performed at the same ICU during 1999–2004.Materials and methodsRetrospective analysis of bacterial blood culture results from neonatal foals ≤7 days of age admitted to Scone Equine Hospital between 2005 and 2022 was analysed. Samples were collected at admission. Antimicrobial sensitivity testing was assessed using the Kirby–Bauer disc diffusion method. Susceptibility results from the two study periods were compared using Fisher's exact tests. Statistical significance was achieved at P value <0.05.ResultsFrom 1621 blood cultures submitted, 380 returned a positive result (380/1621; 23.4%). A total of 402 bacterial isolates were recovered: 55.5% Gram‐positive isolates (223/402), 42.6% Gram‐negative isolates (171/402) and 1.7% anaerobic isolates (7/409). A significant increase in Gram‐positive isolates between time periods was observed (P = 0.002). Gram‐negative isolates had significant increases of resistance in seven out of nine antimicrobials tested.ConclusionThis study provides Australian veterinarians with information on common bacterial pathogens in critically ill neonatal foals to assist with making informed empirical antimicrobial choices to optimise treatment efficacy. Increases in bacterial resistance to commonly administered antimicrobials were observed; therefore, culture and sensitivity testing should guide antimicrobial choices. Judicious use of antimicrobials in equine practice is imperative.
Exploring the congruence between perceived parent–teacher achievement goals and student academic outcomes: A study using polynomial regression with response surface analysis
Authors: Zhao Ningning , Dou Diya , Chen Xiaohan , Chen Fumei , Luo Rui , Zhu Xiaoqin , Xiang Guo‐Xing 
Published: January 2025

AbstractBackgroundThe achievement goals set by parents and teachers play a crucial role in shaping students' personal goal orientation and academic performance. Previous studies have revealed discrepancies between achievement goals set by parents and teachers. However, limited research has examined how the congruence of perceived parents' and teachers' achievement goals is associated with students' academic performance.AimsThe current study sought to investigate the impact of congruence and discrepancy between students' perceptions of teachers' and parents' achievement goals, including mastery goals and performance goals, on students' academic performance in mathematics and language.Sample and MethodsData were collected from a sample of 4944 Chinese students from Grades 3 to 8 using self‐reported questionnaires. Polynomial regression with response surface analyses were employed to analyse the data.ResultsThe results indicated that students' performance in both mathematics and language improved when congruence levels were high in perceived teachers' and parents' mastery goals. Conversely, the congruence level between perceptions of teachers' and parents' performance goals was only related to students' mathematics performance.ConclusionsThe findings underscore the significance of congruence between perceived teachers' and parents' achievement goals in influencing students' academic performance.
EpigenPlot: An interactive web platform for DNA methylation‐based biomarker and drug target discovery in colorectal cancer
Authors: Müller Dalma , Győrffy Balázs 
Published: January 2025

AbstractBackground and PurposeGenome‐wide methylation studies have significantly advanced our understanding of colorectal adenocarcinoma progression and biomarker discovery. Aberrant DNA methylation plays a crucial role in gene expression regulation during cancer transformation, highlighting the need to identify differentially methylated regions (DMRs) as potential diagnostic and therapeutic markers. However, an integrated resource to explore and validate methylation alterations across colorectal cancer stages has been lacking. We aimed to develop a platform that integrates existing methylation data, systematically identifies DMRs and provides a tool for further investigation.Experimental ApproachWe created a database combining Illumina HumanMethylation450K and EPIC data from normal colon, adenoma and adenocarcinoma tissues, comprising 2346 samples from 19 datasets. Methylation levels were analysed in six gene regions, and comparisons between tissue types were made using Mann–Whitney, Kruskal–Wallis and ROC tests.Key ResultsBoth adenoma and adenocarcinoma samples exhibited a general decrease in methylation compared to healthy tissue. Differential methylation in genes such as ITGA4, NPY, IGFL1 and LRRC4 was validated. The strongest DMRs were observed in the C1orf70 gene's 5′UTR and TSS200 regions, with AUC values of 0.98 in both of the HM450K and EPIC datasets. We established an interactive web‐based platform accessible at https://epigenplot.com/ enabling future analysis of individual gene regions.Conclusions and ImplicationsOur study provides an integrated database of DNA methylation profiles across normal, adenoma and adenocarcinoma tissues, offering a valuable resource for biomarker discovery. The integrated web platform can serve as a tool for the development of methylation‐based therapies in the future.
Longitudinal Dyadic Associations Between Maternal Monitoring, Household Environment and Mother–Adolescent Closeness
Authors: Outler Carson , Lowery Dylann F. , McWey Lenore M. , Cui Ming 
Published: January 2025

ABSTRACTThere is a wealth of existing research supporting the importance of mother–adolescent closeness for short‐ and long‐term positive outcomes. Employing the bioecological systems model and the family resilience model, the present study aimed to explore modifiable components of this critical dyad's relationship and shared environment to promote relationship closeness. Longitudinal data from the Future of Families and Child Wellbeing Study were used to explore the associations between maternal monitoring, household environment and relationship closeness for 2795 mother–adolescent dyads via actor–partner interdependence mediation modelling. From both mothers' and youths' perspectives, higher levels of monitoring in middle childhood were positively associated with relationship closeness in adolescence. Further, partner effects were discovered such that one's own perception of monitoring was positively and significantly correlated with the other's report of closeness. Direct pathway, partial mediation, actor and partner effects were found for household environment with relational closeness and maternal monitoring. These findings point to dyadic interactions among maternal monitoring, the household environment and mother–adolescent closeness. It is recommended to consider and attend to the shared context, including the household environment, when working with mothers and youths. Findings also underscore the importance of considering dyadic accounts of the relationship and environment, rather than relying on individual reports.
Colletotrichum siamense Causing Leaf Spot on Black Olive (Bucida buceras) in Mexico
Authors: Cota‐Rodríguez David A. , Beltrán‐Peña Hugo , Vieira Willie A. S. , Camacho‐Tapia Moisés , Lagunes‐Fortiz Erika , Morales‐Gallegos Luis M. , Tovar‐Pedraza Juan M. 
Published: January 2025

ABSTRACTDuring the summer of 2019, spots were detected on the leaves of black olive (Bucida buceras L.) trees distributed in urban areas in Los Mochis, Sinaloa, Mexico. Two single‐spore isolates were obtained and identified as Colletotrichum siamense by phylogenetic analyses of concatenated sequences of the ITS, gapdh, tub2, act, chs‐1 and gs genes. The pathogenicity of the isolates was demonstrated through inoculations under controlled conditions. This is the first report of C. siamense causing leaf spot of black olive in Mexico and worldwide.
Outcome and survival of septic arthritis treated with 0.05% chlorhexidine solution: Description of 10 cases
Authors: Rosa T. C. , Magalhães M. T. , Santos I. , Uva M. , Ramos S. , Pardon Lamas L. R. G. 
Published: January 2025

SummaryBackgroundEstablished or ongoing septic arthritis requiring repeated joint flushing remains a treatment challenge with a poor survival outcome aggravated by the necessity of controlled antimicrobial usage.ObjectiveTo describe the outcomes and survival rates of patients with synovial sepsis treated by lavage with a 0.05% chlorhexidine solution.Study designClinical retrospective observational study.MethodsPatients with synovial sepsis underwent standing needle or arthroscopic lavage under general anaesthesia with a 0.05% chlorhexidine solution, followed by Lactate Ringer Solution (LRS). Complementary systemic and regional antibiotics were used. Short‐ (6 months) and long‐term (>1 year) outcomes were assessed.ResultsTen horses met the inclusion criteria. Affected joints included metacarpophalangeal joints (2), tarsocrural joint (1), radiocarpal and intercarpal joints (2), distal interphalangeal joints and navicular bursa (3), distal interphalangeal joints (2). Time from onset to referral ranged from 1 to 20 days, with a mean of 8.3 days. Eighty per cent of horses survived for more than 1 year. Four horses were retired to the field, three returned to a light level of work and one went back to full work.Main limitationsRetrospective study of a limited group of horses initially treated by different clinicians before referral, as such, first‐line treatment had differences between cases. Resolution was evaluated on based clinical signs rather than clinical pathology of synovial fluid due to clinical decisions or difficulty in obtaining samples.ConclusionWith this series of challenging synovial sepsis cases treated by lavage with a chlorhexidine solution, we provide a treatment protocol, proof of clinical concept and report patients' short‐ and long‐term clinical outcomes. Short‐ and long‐term postoperative lameness should be expected despite the resolution of the septic arthritis. This treatment poses a novel option for challenging cases but should be regarded as a salvage procedure due to the possibility of long‐term mild lameness.
Physical Health Views Among Individuals Experiencing Mental Illness: A Mixed‐Methods Study of Self‐Reported Health and Contributing Factors
Authors: Heinz Stefan Sebastian , O'Brien Anthony John , Parsons Matthew , Walker Cameron 
Published: January 2025

ABSTRACTSevere mental illness is linked to poor physical health and shorter life expectancy, yet research on how individuals experiencing mental illness view and on improve their physical health is limited. This study investigates the perceptions of individuals experiencing mental illness regarding their physical health, utilising a mixed‐methods approach. Phase I involved quantitative and qualitative data from an online Qualtrics survey, which included the 12‐item Short Form (SF‐12) survey to measure participants' quality of life and assess self‐reported physical and mental health. Key findings from Phase I revealed significant relationships between lower Physical Component Summary (PCS) scores and factors such as the frequency of GP visits. Additionally, exercise preferences were found to significantly impact Mental Component Summary (MCS) scores, with individuals who preferred a mix of exercise settings reporting higher MCS scores compared to those who exercised alone or with a training partner. Phase II explored these findings further through semi‐structured interviews, where participants discussed themes including physical health perceptions, the role of medication and the importance of the general practitioner relationship. Thematic analysis revealed five main barriers to improving physical health: accessibility and availability of services, motivation, staff attitudes, medication side effects and the experience of diagnostic overshadowing. Participants reported viewing physical and mental health as interconnected and expressed a desire for more collaborative care. The results suggest that strengthening the relationship with GPs and increasing awareness of medication side effects may improve physical health outcomes for individuals experiencing mental illness. Mental health nurses can play a pivotal role in enhancing physical health outcomes by monitoring, supporting health‐improving strategies and facilitating access to primary care services.
Factors influencing negative outcomes for nurses who experience patient safety incidents: An integrative review
Authors: Lee Hanseulgi , Lee Nam‐Ju , Kim Nari 
Published: January 2025

AbstractAimTo synthesize evidence on factors influencing negative outcomes following patient safety incidents.BackgroundPatient safety incidents affect not only patients and families but also healthcare workers (second victims) and institutions (third victims). Nurses are at risk due to stressful environments and direct patient care, leading to defensive practices, job turnover, and errors. Identifying these factors and developing support strategies are crucial.MethodsThis integrative review followed Whittemore and Knafl's method, guided by the middle‐range theory of nurses’ psychological trauma. Seven databases (PubMed, CINAHL, PsycINFO, Cochrane Library, KISS, KMbase, and RISS) were searched for English and Korean articles from 2014 to 2023. Study quality was evaluated using Joanna Briggs Institute tools, and reporting followed the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses checklist.ResultsA review of 24 cross‐sectional studies from the United States and China revealed factors such as genetic/biological (e.g., gender and age), environmental (e.g., work experience and organizational support), and psychological (e.g., distress and coping). Nonpunitive cultures reduced negative emotions, while high harm and preventable incidents increased negative impacts, mitigated by spirituality. These findings underscore the need for targeted interventions and supportive policies.ConclusionMultidimensional support strategies are necessary to improve nurses' working environments and patient care quality. Further research should explore additional mitigating factors and third victim studies.Implications for nursing policyThis review highlights the need for global mental health strategies and supportive organizational cultures for nurses. Addressing punitive environments and systemic factors through policy changes can improve nurse well‐being and patient care quality. Creating safer environments not only benefits nurses but also enhances the quality and safety of patient care, ultimately driving the provision of better healthcare services.
Indirect Contact and Knowledge Interventions to Improve Relations in the Disabled‐Nondisabled Intergroup Context: A Systematic Review
Authors: Dole Rebecca Jennie , Cameron Lindsey , Abbot‐Smith Kirsten 
Published: January 2025

ABSTRACTStigma and prejudice towards individuals with disabilities is still prevalent in society today (Livneh, Chan, and Kaya 2014). Our aim was to evaluate the state of the research that tests interventions aiming to improve such attitudes, including uncovering which intervention methods can reduce this prejudice and identifying gaps in the research. Since a large proportion of nondisabled individuals will not have the opportunity for direct contact with disabled individuals, our systematic review focuses on non‐direct‐contact interventions, specifically knowledge‐based and indirect contact techniques. Fifty‐one studies published between 2001 and 2022 met all criteria, including the use of a comparison or control group. Overall, most studies did have a positive change on outcome measures, with some maintaining the effect weeks or months later. Despite intervention successes, trends indicating gaps in the research were uncovered including the focus on child and undergraduate student participants, and the lack of collaborative research with the disabled communities.
Prion Protein Endoproteolysis: Cleavage Sites, Mechanisms and Connections to Prion Disease
Authors: Castle Andrew R. , Westaway David 
Published: January 2025

ABSTRACTHighly abundant in neurons, the cellular prion protein (PrPC) is an obligatory precursor to the disease‐associated misfolded isoform denoted PrPSc that accumulates in the rare neurodegenerative disorders referred to either as transmissible spongiform encephalopathies (TSEs) or as prion diseases. The ability of PrPC to serve as a substrate for this template‐mediated conversion process depends on several criteria but importantly includes the presence or absence of certain endoproteolytic events performed at the cell surface or in acidic endolysosomal compartments. The major endoproteolytic events affecting PrPC are referred to as α‐ and β‐cleavages, and in this review we outline the sites within PrPC at which the cleavages occur, the mechanisms potentially responsible and their relevance to pathology. Although the association of α‐cleavage with neuroprotection is well‐supported, we identify open questions regarding the importance of β‐cleavage in TSEs and suggest experimental approaches that could provide clarification. We also combine findings from in vitro cleavage assays and mass spectrometry‐based studies of prion protein fragments in the brain to present an updated view in which α‐ and β‐cleavages may represent two distinct clusters of proteolytic events that occur at multiple neighbouring sites rather than at single positions. Furthermore, we highlight the candidate proteolytic mechanisms best supported by the literature; currently, despite several proteases identified as capable of processing PrPC in vitro, in cell‐based models and in some cases, in vivo, none have been shown conclusively to cleave PrPC in the brain. Addressing this knowledge gap will be crucial for developing therapeutic interventions to drive PrPC endoproteolysis in a neuroprotective direction. Finally, we end this review by briefly addressing other cleavage events, specifically ectodomain shedding, γ‐cleavage, the generation of atypical pathological fragments in the familial prion disorder Gerstmann–Sträussler–Scheinker syndrome and the possibility of an additional form of endoproteolysis close to the PrPC N‐terminus.image
GABAB Receptor Modulation of Membrane Excitability in Human Pluripotent Stem Cell‐Derived Sensory Neurons by Baclofen and α‐Conotoxin Vc1.1
Authors: St Clair‐Glover Mitchell , Yousuf Arsalan , Kaul Dominic , Dottori Mirella , Adams David J. 
Published: January 2025

ABSTRACTGABAB receptor (GABABR) activation is known to alleviate pain by reducing neuronal excitability, primarily through inhibition of high voltage‐activated (HVA) calcium (CaV2.2) channels and potentiating G protein–coupled inwardly rectifying potassium (GIRK) channels. Although the analgesic properties of small molecules and peptides have been primarily tested on isolated murine dorsal root ganglion (DRG) neurons, emerging strategies to develop, study, and characterise human pluripotent stem cell (hPSC)‐derived sensory neurons present a promising alternative. In this study, hPSCs were efficiently differentiated into peripheral DRG‐induced sensory neurons (iSNs) using a combined chemical and transcription factor‐driven approach via a neural crest cell intermediate. Molecular characterisation and transcriptomic analysis confirmed the expression of key DRG markers such as BRN3A, ISLET1, and PRPH, in addition to GABABR and ion channels including CaV2.2 and GIRK1 in iSNs. Functional characterisation of GABABR was conducted using whole‐cell patch clamp electrophysiology, assessing neuronal excitability under current‐clamp conditions in the absence and presence of GABABR agonists baclofen and α‐conotoxin Vc1.1. Both baclofen (100 μM) and Vc1.1 (1 μM) significantly reduced membrane excitability by hyperpolarising the resting membrane potential and increasing the rheobase for action potential firing. In voltage‐clamp mode, baclofen and Vc1.1 inhibited HVA Ca2+ channel currents, which were attenuated by the selective GABABR antagonist CGP 55845. However, modulation of GIRK channels by GABABRs was not observed in the presence of baclofen or Vc1.1, suggesting that functional GIRK1/2 channels were not coupled to GABABRs in hPSC‐derived iSNs. This study is the first to report GABABR modulation of membrane excitability in iSNs by baclofen and Vc1.1, highlighting their potential as a future model for studying analgesic compounds.image
Assessing Clinical Nurse Educators’ Perceptions of Factors Influencing Evidence‐Based Nursing Policy Implementation Within a Large Pediatric Healthcare System
Authors: Lecesne Hannah , Kimble Laura , Gettis Margaret , Dye Betsy 
Published: January 2025

ABSTRACTAimTo identify barriers and facilitators to implementation of new evidence‐based nursing policies among nurse educators.BackgroundEvidence‐based practice promotes safe patient care through the combination of the best available research, clinical expertise and patient preferences. Policies are utilised by nursing to drive patient care and thus should be evidence‐based. Nurse educators are identified as champions of evidence‐based practice and policy dissemination. Therefore, understanding nurse educators' perceptions surrounding barriers and facilitators to nursing policy implementation and attitudes regarding evidence‐based practice can help drive successful policy implementation and foster evidence‐based practice in patient care.DesignDescriptive, cross‐sectional.MethodsA 7‐item survey was electronically distributed to all nurse educators within a large pediatric healthcare system to assess barriers and facilitators to policy implementation as well as attitudes surrounding evidence‐based practice and policies.FindingsThe most significant barriers were lack of time and poor understanding of policy development. The most significant facilitators were a standardised policy dissemination process and leadership support of policy implementation efforts. Most nurse educators agreed that evidence‐based practice is valuable to patient care. However, nurse educators report that they have a neutral to poor understanding of evidence‐based practice.ConclusionFindings were consistent with the literature, thus adding to the body of literature that supports the need for further development around policy dissemination and evidence‐based practice education for nurse educators.Implications for the Profession and/or Patient CareThis paper supports the need for professional development for nurse educators, standardisation of policy dissemination and leadership support of policy dissemination and promotion of evidence‐based practice.Patient or Public ContributionNo patient or public contribution.Reporting MethodThis paper adheres to the SQUIRE 2.0 guidelines.