bluuebunny/crossref_metadata_2025_split huggingface dataset:
Volatile Compound analysis of ageing Pinus sylvestris L. (Scots pine) seeds
Authors: Tammela P. , Nygren M. , Laakso I. , Hopia A. , Vuorela H. , Hiltunen R. 
Published: July 2003

AbstractThe volatile compound proﬁles of 11 Scots pine (Pinus sylvestris L.) seed lots were determined by solid phase microextraction combined with GC–MS analysis. Typical monoterpene constituents of pine volatile oils were identiﬁed, and total monoterpene content of these seeds was measured semi‐quantitatively as peak area. Considerable variation in monoterpene composition was found between seeds of different origin, especially in two major constituents, 3‐carene and α‐pinene. The relative concentrations of these compounds varied between 0–67 and 16–52%, respectively. Comparison of total monoterpene amounts showed that reduced monoterpene content was typical for aged, poorly germinating seeds. However, high germinability was not always associated with a high monoterpene content. Therefore, no clear dependency between monoterpene content and seed ageing could be established based on this data. The possible protective role of monoterpenes in pine seed ageing during long‐term storage is discussed. In addition, a drastic increase in volatile lipid oxidation products was found in the oldest seed lots. In 20 year‐old seeds, the percentage of hexanal, an oxidation product of fatty acids, was 3% of the total volatiles, whereas in 31 year‐old seeds it was 17%. No hexanal or other lipid oxidation products were detected in younger seeds. Copyright © 2003 John Wiley & Sons, Ltd.
Volatile constituents from leaf, inﬂorescence and root oils of Ocimum americanum L. grown in north‐eastern Brazil
Authors: De Vasconcelos Silva M. G. , Dos Santos R. N. , Abreu Matos F. J. , Machado M. I. L. 
Published: July 2003

AbstractChemical composition of essential oils obtained by microwave and steam distillations from several parts of Ocimum americanum L. growing in north‐eastern Brazil were determined by GC–MS and retention time analysis. (E)‐Methyl cinnamate was found to be the major constituent in the leaves (82.7–82.7%) and inﬂorescence (87.1–97.5%), respectively, while eugenol, which was not traceable in these parts, reaches 88.2% in the root essential oil. In view of these data O. americanum from north‐eastern Brazil today represented a novel chemotype and would appear to be akin to the African, Commodore Island and French Congo plants, as well as to the US and Nigerian plants. Copyright © 2003 John Wiley & Sons, Ltd.
Aromatic Plants of Tropical Central Africa. Part XLIII Volatile Components from Uvariastrum pierreanum Engl. (Engl. & Diels) Growing in Cameroon
Authors: Boyom F. Fekam , Amvam Zollo P. H. , Agnaniet H. , Menut C. , Bessière J. M. 
Published: July 2003

AbstractThe essential oils obtained from the leaves and stem bark of Uvariastrum pierreanum Engl. (Engl. & Diels) (Annonaceae), collected in Cameroon, were analysed by GC and GC–MS. The leaf oil was found to contain a majority of monoterpenes, with α‐ and β‐pinenes (43.8%) as the major constituents, while the stem bark extract comprised sesquiterpenoids only. In this class of compounds, the leaf and stem bark oils were found to be qualitatively similar, with a predominance of bisabolane (35.9%) and bergamotane (23.9%)‐type structures. None of these two samples revealed interesting antiradical activity. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oil composition of Cymbopogon martinii from different places in India
Authors: Raina V. K. , Srivastava S. K. , Aggarwal K. K. , Syamasundar K. V. , Khanuja S. P. S. 
Published: July 2003

AbstractThe essential oils of Palmarosa (Cymbopogon martinii (Roxb.) Wats. var. motia Burk) ﬂowering herbs from three different geographical locations in India, viz. Hyderabad,Lucknow and Amarawati were analysed by high‐resolution GC and GC –MS. In all three samples, geraniol (67.6 –83.6%) was the major constituent and, although the composition of the three oils was similar, quantitative differences in the concentration of some constituents were observed. The GC and GC –MS analysis of the Hyderabad, Lucknow and Amarawati oils resulted in the identiﬁcation of 40, 38 and 33 constituents, representing 98.5%, 99.7% and 99.8% of the oils,respectively. Among the three oils analysed, Amarawati oil was of the highest quality due to higher geraniol (83.6%) and lower geranyl acetate (2.3%) and geranial (1.0%) content. Copyright © 2003 John Wiley & Sons, Ltd.
Supercritical carbon dioxide extraction of Foeniculum vulgare volatile oil
Authors: Coelho J. A. P. , Pereira A. P. , Mendes R. L. , Palavra A. M. F. 
Published: July 2003

AbstractSupercritical ﬂuid extraction (SFE) with CO2 of the volatile oil from fennel (Foeniculum vulgare ssp. piperitum (Ucria) Coutinho) was carried out at temperatures of 40 and 50 °C and pressures of 90 and 100 bar in a ﬂow apparatus using a two‐stage fractional separation technique. The best conditions for extraction (pressure P = 90 bar, and temperature, T = 40 °C)and separation (P = 80 bar, T = −10 °C for the ﬁrst separator and P = 20 bar, T = −10 °C for the second)were used to assess the effect of different mean particle size and ﬂow rate of CO2. The yield of the extraction and composition of the volatile oil were compared with those obtained by hydrodistillation. The study showed that the particle size of the fruits does not practically affect either the yield or the composition of the oil, with the exception of the presence of a small amount of waxes in the volatile oil extracted by SFE from the fruits presenting the highest particle size. Further, increasing of the ﬂow rate of CO2 does not seem to inﬂuence the composition, although it increases the rate of extraction, thus leading to a decrease of the extraction time. The fennel oil was analysed by gas chromatography (GC) and chromatography –mass spectrometry (GC –MS). The main compounds identiﬁed in the oils were fenchone (17%), estragol (21%) and (E )‐anethole (43%). Copyright © 2003 John Wiley & Sons, Ltd.
Determination of the aroma impact compounds in heated sweet cream butter
Authors: Peterson D. G. , Reineccius G. A. 
Published: July 2003

AbstractThis study was conducted to determine which volatile compounds are primarily responsible for the aroma of heated sweet cream butter. Static headspace analysis was used for aroma isolation and gas chromatography–olfactometry (GC–O) for the selection of odour‐active components. Quantiﬁcation of selected odourants was done via purge and trap‐GC/mass spectrometry (MS). Nineteen odour‐active compounds were detected in the headspace (static) of heated butter (hydrogen sulphide, methanethiol, acetaldehyde, 2,3‐butanedione, 1‐hexen‐3‐one, butanoic acid, 3‐methylbutanoic acid, 2‐heptanone, methional, dimethyl trisulphide, 1‐octen‐3‐one, hexanoic acid, furaneol, δ‐hexanolactone, nonanal, (E)‐2‐nonenal, δ‐octanolactone, skatole and δ‐decanolactone). Aroma recombination studies followed by sensory analysis indicated that the aroma of our heated butter model was rated the same (similarity) as a heated commercial butter (obtained from local market) in comparison to a heated freshly manufactured butter. The aroma of a heated commercial unsalted butter and a heated butter aroma model of Budin
1 also were signiﬁcantly less similar than the aroma of our heated butter model in comparison to a heated freshly manufactured butter. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oil composition of Turkish herbal tea (Salvia aucheri Bentham var. canescens Boiss. & Heldr.)
Authors: Özcan Mussa , Tzakou Olga , Couladis Maria 
Published: July 2003

AbstractThe essential oil of the aerial parts of Salvia aucheri var. canescens, collected from two different locations (Gülnar, sample A; and Silifke, sample B) growing endemic in Turkey, were analysed by GC–MS. The oil yields of dried plants (v/dw) obtained by hydrodistillation were 1.4% and 1.7%, respectively. Forty‐two compounds were identiﬁed, representing 98.6% and 99.5% of the oils from the two locations. The main constituents of the oils were: 1,8‐cineole (32.3%, 28.6%), camphor (18.9%, 22.8%), borneol (8.2%, 8.9%), α‐pinene (6.3%, 9.0%) and β‐pinene (5.3%, 6.2%), respectively. The oils consisted of monoterpenic hydrocarbons (21.3%, 27.4%), oxygenated monoterpenes (66.4%, 64.9%), sesquiterpene hydrocarbons (6.3%, 3.1%) and oxygenated sesquiterpenes (4.1%, 4.1%), respectively. 1,8‐Cineole and camphor were the major components of S. aucheri. var. canescens. Copyright © 2003 John Wiley & Sons, Ltd.
Volatile constituents of benzoin gums: Siam and Sumatra. Part 1
Authors: Fernandez Xavier , Lizzani‐Cuvelier Louisette , Loiseau André‐Michel , Périchet Christine , Delbecque Claire 
Published: July 2003

AbstractThe volatile extract composition of two different benzoin gums, Siam and Sumatra, were analysed by GC–MS. Twenty components representing more than 99.1% of the oil from Siam and 29 components representing more than 97.4% of the oil from Sumatra were analysed. The major components were benzyl benzoate (76.1–80.1%) for the two oils and benzoic acid (12.5%), methyl benzoate (1.5%) and allyl benzoate (1.5%) for Siam, and styrene (2.3%), cinnamic acid (3.5%) and benzyl cinnamate (3.3%) for Sumatra. Volatile compounds of oils and crushed benzoins were also studied using solid‐phase microextraction (SPME) employing carboxen/polydimethylsiloxane and carbowax/divinylbenzene ﬁbres. Copyright © 2003 John Wiley & Sons, Ltd.
Effect of organic agriculture practices on the volatile aroma components of some essential oil plants growing in Egypt II: sweet Marjoram (Origanum marjorana L.) essential oil
Authors: Edris Amr E. , Shalaby Ahmad , Fadel Hoda M. 
Published: July 2003

AbstractSweet marjoram (German type) was grown in Egypt under the conditions of organic and traditional agriculture. Mature compost was used as an organic fertilizer, while ammonium sulphate was used as a chemical fertilizer. Two levels of each fertilizer were used during plant growth. The crops were harvested and hydrodistilled in June and September, the isolated essential oil was fractionated into oxygenated and hydrocarbon fractions, then each fraction was analysed by GC and GC–MS. The results showed that the fresh herb of marjoram plants positively responded to increased levels of either organic or chemical fertilizers. The herb yield was the highest in the September harvest. Although the higher level of organic fertilization negatively affected the oil content in the fresh herb, however, the total oil yield per plant remained comparable with the lower levels of both organic or chemical fertilization. Regarding the oil composition, the results showed that marjoram (German type) grown in Egypt belongs to the cis‐sabinene hydrate/terpinene‐4‐ol chemotype. The chemical composition of marjoram essential oil did not change due to the fertilization type or level, but rather the relative percentage of certain constituents were affected. The highest concentration of cis‐sabinene hydrate (24.1%), under organic treatment was reported for a June crop fertilized at level I, while the highest concentration of terpinene‐4‐ol, (39.5%), under the same treatment, was detected in a June crop at level II fertilization. Other components showed different concentrations depending on season of harvesting, treatment, and level of fertilization. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oil compounds of Origanum dayi Post
Authors: Dudai Nativ , Larkov Olga , Chaimovitsh David , Lewinsohn Efraim , Freiman Lilia , Ravid Uzi 
Published: July 2003

AbstractThe essential oils of ﬁve selected clones of Origanum dayi Post were obtained using two techniques, hydrodistillation and tert‐butyl methyl ether extraction. Hydrodistillation of the whole plant yielded essential oil amounting to 2.4–2.8% of the fresh weight. A total of 50 volatile constituents of the oil were identiﬁed, of which the major ones were 1,8‐cineole, α‐terpineol, (E)‐sabinene hydrate, (E)‐sabinene hydrate acetate, terpinen‐4‐ol and linalyl acetate. The composition of the volatiles obtained by hydrodistillation and by extraction differed mainly in the higher contents of terpinen‐4‐ol (5.7–14.5%) and α‐terpineol (18.3–25.8%) in the distilled oil than in the extract (1.9–4.0% and 10.5–16.8%, respectively), but the former had less (E)‐sabinene hydrate acetate (traces–0.2%) than the latter (6.3–13.8%). Extraction of young and old leaves, young and old stems, bracts, calices and corollas revealed differences among the various plant parts in their volatile contents and compositions. Copyright © 2003 John Wiley & Sons, Ltd.
Seasonal variation in the composition of the essential oils from the leaves, thin branches and resin of Protium spruceanum (Benth.) Engl.
Authors: Machado Líbia Bentes , Zoghbi Maria das Graças B. , Andrade Eloisa Helena A. 
Published: July 2003

AbstractIn a previous paper, we described the essential oils of Protium spruceanum obtained from different parts of the plant. In this paper, the essential oils obtained from the leaves, thin branches and resins were collected from October 1999 at 1 month intervals up to October 2000. At each sampling, the oil content was determined in a Clevenger‐type apparatus, and examined by GC–MS. The major component from the leaf, resin and thin branch oils was sabinene (leaves, January 81.4%; thin branches, January 79.8%; resin, October 1999, 61.3%), followed by β‐caryophyllene in the leaf oil. The maximum content of β‐caryophyllene from the leaf oil was 36.4% in February. The highest oil yields were observed from leaves (0.4%) sampled in April and from resin sampled in October 1999 (4.0%). Thin branch oil yields were in the range ≤0.1–0.2%. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oil composition of Artemisia parviﬂora aerial parts
Authors: Rana Virendra S. , Juyal Jitendra P. , Blazquez M. Amparo , Bodakhe Surendra H. 
Published: July 2003

AbstractThe chemical composition of the essential oil from aerial parts of A. parviﬂora was analysed by GC and GC–MS. Twenty‐two compounds, accounting for 72.7% of the oil, were identiﬁed. The main components identiﬁed were β‐caryophyllene (15.3%), germacrene D (14.7%), camphor (11.4%), artemisia ketone (7.8%), 1,8‐cineole (5.8%), α‐copaene (2.6%), artemisia alcohol (2.6%), terpinene‐4‐ol (2.3%), caryophyllene oxide (1.2%), α‐pinene (1.1%), sabinyl acetate (1.1%) and α‐humulene (1.1%). Copyright © 2003 John Wiley & Sons, Ltd.
Multipoint methylation analysis indicates a distinctive epigenetic phenotype among testicular germ cell tumors and testicular malignant lymphomas
Authors: Kawakami Takahiro , Okamoto Keisei , Kataoka Akira , Koizumi Shuichi , Iwaki Hideaki , Sugihara Hiroyuki , Reeve Anthony E. , Ogawa Osamu , Okada Yusaku 
Published: July 2003

AbstractHypermethylation of tumor‐suppressor genes has been implicated in the pathogenesis of human cancers. Although a growing number of genes showing hypermethylation is being reported in human cancer, methylation profiles of tumor‐related genes in testicular neoplasms have not been well elucidated. This study was designed to show the methylation profiles of multiple CpG islands in testicular germ cell tumors (TGCTs) in comparison with those in testicular malignant lymphomas. We studied the methylation status of E‐cadherin, CDKN2B, CDKN2A, BRCA1, RB1, VHL, RASSF1A, RARB, and GSTP1 by use of TGCT tissues and testicular malignant lymphoma tissues (25 primary TGCT tissues and three primary testicular lymphoma tissues). Methylation was not observed in E‐cadherin, CDKN2B, CDKN2A, BRCA1, RB1, VHL, RASSF1A, RARB, and GSTP1 in any of the TGCT tissues. In contrast, all three (100%) of the testicular lymphoma tissues demonstrated hypermethylation of E‐cadherin, RASSF1A, and RARB, but not CDKN2B, CDKN2A, BRCA1, RB1, VHL, and GSTP1. These data demonstrate that a distinctive epigenetic phenotype underlies the TGCTs and testicular lymphomas at the CpG sites of E‐cadherin, RASSF1A, and RARB; a distinctive epigenetic phenotype was not observed among seminomatous TGCTs and non‐seminomatous TGCTs at the CpG sites examined. © 2003 Wiley‐Liss, Inc.
Both NUP98/TOP1 and TOP1/NUP98 transcripts are detected in a de novo AML with t(11;20)(p15;q11)
Authors: Iwase Satsuki , Akiyama Nobutake , Sekikawa Tetsuaki , Saito Shinobu , Arakawa Yasuhiro , Horiguchi‐Yamada Junko , Yamada Hisashi 
Published: July 2003

AbstractThe NUP98 gene is involved in several chromosomal abnormalities associated with acute leukemia. The recurrent t(11;20)(p15;q11) chromosomal translocation results in generation of the NUP98/TOP1 chimeric gene. This abnormality has been observed primarily in therapy‐related leukemias, and TOP1/NUP98 transcripts have not been demonstrated. We describe a case of de novo acute myeloid leukemia with t(11;20)(p15;q11), with no known history of exposure to chemicals. The translocation occurred in intron 13 of NUP98 and intron 7 of TOP1, as in the three previously reported cases. The breakpoint in NUP98 was exactly the same as that found in a previously reported case. In addition, a reciprocal TOP1/NUP98 transcript was detected for the first time in our patient. © 2003 Wiley‐Liss, Inc.
HMGA2 expression in uterine leiomyomata and myometrium: Quantitative analysis and tissue culture studies
Authors: Gross Karen L. , Neskey David M. , Manchanda Nitasha , Weremowicz Stanislawa , Kleinman Michael S. , Nowak Romana A. , Ligon Azra H. , Rogalla Piere , Drechsler Klaus , Bullerdiek Jörn , Morton Cynthia C. 
Published: July 2003

AbstractThe high mobility group gene, HMGA2, is frequently expressed in uterine leiomyomata (UL) with chromosomal rearrangements of 12q15. In contrast, HMGA2 expression has not been detected in karyotypically normal UL or in myometrium, but has been detected in these tissues after culture. To characterize further the expression pattern of HMGA2, we assessed HMGA2 expression by RT‐PCR followed by Southern blot hybridization, and by real‐time PCR in three tissue panels: (1) primary myometrial cultures, (2) uncultured tissue from 15 karyotypically normal samples consisting of eleven 46,XX UL and four matched myometrial specimens, and (3) uncultured tissue from ten UL with 12q15 rearrangements and three matched myometrial specimens. HMGA2 expression was detected in all samples from the three panels. The level of HMGA2 expression in karyotypically normal UL was similar to the level of expression in myometrium; however, it was significantly less than the level measured in UL with 12q15 rearrangements. This expression analysis by use of detection methods of different sensitivities underscores the importance of studies of HMGA2 expression in uncultured tissues and of careful interpretation of results from experiments on cultured cells. Moreover, detection of HMGA2 expression in myometrium and in UL without 12q15 rearrangements, tissues previously thought not to express HMGA2, suggests that HMGA2 expression is required in normal adult myometrial physiology. © 2003 Wiley‐Liss, Inc.
Mutations of the BIK gene in human peripheral B‐cell lymphomas
Authors: Arena Vincenzo , Martini Maurizio , Luongo Myriam , Capelli Arnaldo , Larocca Luigi Maria 
Published: July 2003

AbstractBIK, a BH (Bcl2 homology domain)3‐only protein, is a proapoptotic member of the BCL2 family. We performed single‐strand conformational polymorphism and sequencing analysis of the entire coding region of the BIK gene (exons 2–5) in 71 B‐cell lymphomas [27 follicular lymphomas (FLs), 13 marginal cell lymphomas (MZLs), 7 small lymphocytic lymphomas (SLLs), 6 mantle cell lymphomas (MCLs), 2 lymphoplasmacytic lymphomas, and 16 diffuse large B‐cell lymphomas (DLBCLs)]. Missense BIK gene mutations were observed in 3 of 27 (11%) FLs, in 2 of 13 (15%) MZLs, and in 1 of 16 (6%) DLBCLs. Sequence alterations in intronic regions were observed in 4 of 27 (14.8%) FLs, in 7 of 13 (53%) MZLs, and in 3 of 16 (18%) DLBCLs. These data indicate that mutation of the BIK gene is a frequent feature of B‐cell lymphomas. © 2003 Wiley‐Liss, Inc.
AML1/RUNX1 mutations are infrequent, but related to AML‐M0, acquired trisomy 21, and leukemic transformation in pediatric hematologic malignancies
Authors: Taketani Takeshi , Taki Tomohiko , Takita Junko , Tsuchida Masahiro , Hanada Ryoji , Hongo Teruaki , Kaneko Takashi , Manabe Atsushi , Ida Kohmei , Hayashi Yasuhide 
Published: July 2003

AbstractAML1/RUNX1, located on chromosome band 21q22, is one of the most important hematopoietic transcription factors. AML1 is frequently affected in leukemia and myelodysplastic syndrome with 21q22 translocations. Recently, AML1 mutations were found in adult hematologic malignancies, especially acute myeloid leukemia (AML)–M0 or leukemia with acquired trisomy 21, and familial platelet disorder with a predisposition toward AML. Through the use of polymerase chain reaction–single‐strand conformation polymorphism analysis, we examined the AML1 gene for mutations in 241 patients with pediatric hematologic malignancies, and we detected AML1 mutations in seven patients (2.9%). Deletion was found in one patient, and point mutations in four patients, including three missense mutations, two silent mutations, and one mutation within an intron resulting in an abnormal splice acceptor site. All of the mutations except for one were heterozygous. Mutations within the runt domain were found in six of seven patients. Six of seven patients with AML1 mutations were diagnosed with AML, and one had acute lymphoblastic leukemia. In three of these seven patients, AML evolved from other hematologic disorders. AML1 mutations were found in two of four AML‐M0 and two of three patients with acquired trisomy 21. Patients with AML1 mutations tended to be older children. Three of four patients with AML1 mutations who received stem cell transplantation (SCT) are alive, whereas the remaining three patients with mutations without SCT died. These results suggest that AML1 mutations in pediatric hematologic malignancies are infrequent, but are possibly related to AML‐M0, acquired trisomy 21, and leukemic transformation. These patients may have a poor clinical outcome. © 2003 Wiley‐Liss, Inc.
Paleoecology and geochronology of glacial Lake Hind during the Pleistocene–Holocene transition: A context for Folsom surface finds on the Canadian Prairies
Authors: Boyd Matthew , Running Garry Leonard , Havholm Karen 
Published: July 2003

AbstractStratigraphic and paleoecologic (palynomorph, macrobotanical) data obtained from a cutbank of the Souris River in southwestern Manitoba establish some fundamental parameters of Folsom land‐use in association with a proglacial lake on the Canadian Prairies. By dating the regression of glacial Lake Hind, we observed that recorded Folsom sites are restricted to areas of the Hind basin drained shortly before 10,400 yr B.P. This pattern may therefore record the interception of seasonal resources on recently‐drained proglacial lake surfaces. Based on paleovegetation reconstructions, we note that these surfaces were rapidly colonized by emergent and aquatic vegetation following regression, generating a viable resource base for Folsom hunter‐gatherers. However, low plant productivity and diversity may have greatly limited the extent to which this locale was exploited, in contrast to nonperiglacial regions on the Plains. We also suggest that wetland plant succession during the Pleistocene‐Holocene transition was due, at least locally, to climate‐forced fluctuations in groundwater levels. © 2003 Wiley Periodicals, Inc.