bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of Mitochondrial Transition Pore Components by Thyroid Hormone
Authors: Yehuda-Shnaidman Einav , Kalderon Bella , Bar-Tana Jacob 
Published: February 2005

AbstractThyroid hormone (TH) modulates metabolic efficiency by controlling the coupling of mitochondrial oxidative phosphorylation. However, its uncoupling mode of action is still enigmatic. Treatment of Jurkat or GH3 cells with T3 is reported here to result in limited, Cyclosporin A-sensitive mitochondrial depolarization, conforming to low conductance gating of the mitochondrial transition pore (MTP). MTP protein components induced by T3 treatment were verified in T3-treated and hypothyroid rat liver as well as in Jurkat cells. T3 treatment resulted in increase in mitochondrial Bax and Bak together with decreased mitochondrial Bcl2. T3-induced mitochondrial depolarization was aborted by overexpression of Bcl2. In contrast to Bax-Bcl2 family proteins, some other MTP components were either not induced by T3 (e.g. voltage-dependent anion channel) or were induced, but were not involved in Cyclosporin A-sensitive MTP gating (e.g. Cyclophilin D and adenine nucleotide translocase-2) Hence, TH-induced mitochondrial uncoupling may be ascribed to low conductance MTP gating mediated by TH-induced increase in mitochondrial proapoptotic combined with a decrease in mitochondrial antiapoptotic proteins of the Bax-Bcl2 family.
The Receptor for Parathyroid Hormone and Parathyroid Hormone-Related Peptide Is Hydrolyzed and Its Signaling Properties Are Altered by Directly Binding the Calpain Small Subunit
Authors: Shimada Masako , Mahon Matthew J. , Greer Peter A. , Segre Gino V. 
Published: February 2005

Abstract
We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with the endogenous calpain small subunit in HEK293 cells. Calpain hydrolyzes ΔNt-rPTH1R, a receptor with a 156-amino acid N-terminal deletion, in a calcium-dependent manner in vitro and in intact cells. Most importantly, PTH stimulation increases the cleavage of ΔNt-rPTH1R and rPTH1R-yellow fluorescent protein in HEK293 cells, and of talin in HEK293 cells expressing rPTH1R-yellow fluorescent protein and in ROS17/2.8 osteoblast-like cells that express rPTH1R endogenously. The absence of calpain in Capn4-null embryonic fibroblasts and the lowered calpain activity in MC3T3-E1 osteoblastic cells due to stable expression of the calpain inhibitor, calpastatin, reduce PTH-stimulated cAMP accumulation. The calpain small subunit is the second protein, in addition to the sodium-hydrogen exchanger regulatory factor, and the first enzyme that binds the PTH1R; PTH1R bound to both of these proteins results in altered PTH signaling.
Cobalt Chloride-Induced Estrogen Receptor α Down-Regulation Involves Hypoxia-Inducible Factor-1α in MCF-7 Human Breast Cancer Cells
Authors: Cho Jungyoon , Kim Dukkyung , Lee SeungKi , Lee YoungJoo 
Published: February 2005

Abstract
The estrogen receptor (ER) is down-regulated under hypoxia via a proteasome-dependent pathway. We studied the mechanism of ERα degradation under hypoxic mimetic conditions. Cobalt chloride-induced ERα down-regulation was dependent on the expression of newly synthesized protein(s), one possibility of which was hypoxia-inducible factor-1α (HIF-1α). To examine the role of HIF-1α expression in ERα down-regulation under hypoxic-mimetic conditions, we used a constitutively active form of HIF-1α, HIF-1α/herpes simplex viral protein 16 (VP16), constructed by replacing the transactivation domain of HIF-1α with that of VP16. Western blot analysis revealed that HIF-1α/VP16 down-regulated ERα in a dose-dependent manner via a proteasome-dependent pathway. The kinase pathway inhibitors PD98059, U0126, wortmannin, and SB203580 did not affect the down-regulation. A mammalian two-hybrid screen and immunoprecipitation assays indicated that ERα interacted with HIF-1α physically. These results suggest that ERα down-regulation under hypoxia involves protein-protein interactions between the ERα and HIF-1α.
Unmodified Prolactin (PRL) and S179D PRL-Initiated Bioluminescence Resonance Energy Transfer between Homo- and Hetero-Pairs of Long and Short Human PRL Receptors in Living Human Cells
Authors: Tan Dunyong , Johnson David A. , Wu Wei , Zeng Lingfang , Chen Yen Hao , Chen Wen Y. , Vonderhaar Barbara K. , Walker Ameae M. 
Published: February 2005

AbstractWe have used bioluminescence resonance energy transfer (BRET) to examine the interaction between human prolactins (PRLs) and the long (LF) and two short isoforms (SF1a and SF1b) of the human PRL receptor in living cells. cDNA sequences encoding the LF, SF1a, and SF1b were subcloned into codon-humanized vectors containing cDNAs for either Renilla reniformis luciferase (Rluc) or a green fluorescent protein (GFP2) with a 12- or 13-amino acid linker connecting the parts of the fusion proteins. Transfection into human embryonic kidney 293 cells demonstrated maintained function of Rluc and GFP2 when linked to the receptors, and confocal microscopy demonstrated the localization of tagged receptors in the plasma membrane by 48 h after transfection. All three tagged receptors transduced a signal, with the LF and SF1a stimulating, and SF1b inhibiting, promoter activity of an approximately 2.4-kb β-casein-luc construct. Both unmodified PRL (U-PRL) and the molecular mimic of phosphorylated PRL, S179D PRL, induced BRET with all combinations of long and short receptor isoforms except SF1a plus SF1b. No BRET was observed with the site two-inactive mutant, G129R PRL. This is the first demonstration, 1) that species homologous PRL promotes both homo- and hetero-interaction of most long and short PRLR pairs in living cells, 2) that both U-PRL and S179D PRL are active in this regard, and 3) that there is some aspect of SF1a-SF1b structure that prevents this particular hetero-receptor pairing. In addition, we conclude that preferential pairing of different receptor isoforms is not the explanation for the different signaling initiated by U-PRL and S179D PRL.
Three’s Company: Two or More Unrelated Receptors Pair with the Same Ligand
Authors: Ben-Shlomo Izhar , Hsueh Aaron J. W. 
Published: February 2005

AbstractIntercellular communication relies on signal transduction mediated by extracellular ligands and their receptors. Although the ligand-receptor interaction is usually a two-player event, there are selective examples of one polypeptide ligand interacting with more than one phylogenetically unrelated receptor. Likewise, a few receptors interact with more than one polypeptide ligand, and sometimes with more than one coreceptor, likely through an interlocking of unique protein domains. Phylogenetic analyses suggest that for certain triumvirates, the matching events could have taken place at different evolutionary times. In contrast to a few polypeptide ligands interacting with more than one receptor, we found that many small nonpeptide ligands have been paired with two or more plasma membrane receptors, nuclear receptors, or channels. The observation that many small ligands are paired with more than one receptor type highlights the utilitarian use of a limited number of cellular components during metazoan evolution. These conserved ligands are ubiquitous cell metabolites likely favored by natural selection to establish novel regulatory networks. They likely possess structural features useful for designing agonistic and antagonistic drugs to target diverse receptors.
Traumatic Atlanto-occipital Dislocation: Presentation of a New Posterior Occipitoatlantoaxial Fixation Technique in an Adult Survivor: Technical Case Report
Authors: Payer Michael , Sottas Cyrille C. 
Published: February 2005

AbstractOBJECTIVE AND IMPORTANCE:More survivors of traumatic atlanto-occipital dislocation (AOD) in adults have recently been reported. Surgical management options are therefore of increasing interest. We present a new technique of posterior C0–C1–C2 fixation.CLINICAL PRESENTATION:A 29-year-old motorcyclist survived a traumatic vertical AOD of 15 mm. No spinal cord or medullary lesion was present. Brain contusion and diffuse axonal injuries led to a cortical biplegia, which recovered progressively over a period of 6 months. Twelve months after surgery, no neurological deficit was present except for slightly increased deep tendon reflexes.INTERVENTION:Posterior C0–C1–C2 fixation was performed with two bicortical occipital screws, one bicortical lateral mass screw in the atlas, and one monocortical pars screw in the axis on each side, connected to a plate-rod on the right and left sides. Fusion was performed with monocortical bone graft from the posterior iliac crest.CONCLUSION:The surgical technique described was thought to be safe to perform and resulted in immediate stability without external immobilization. Solid fusion was achieved 6 months after surgery.
Frameless Stereotaxy to Facilitate Anterolateral Thoracolumbar Surgery: Technique
Authors: Chappell E Thomas , Pare Laura , Dolich Matthew O. , Lekawa Michael E. , Salepour Mohammed 
Published: February 2005

Abstract
WITH THE ADVENT of frameless stereotaxy and its application to the spine, more precise and less invasive spinal procedures are possible. In addition to being less invasive, these techniques may increase surgeon confidence and allow shorter operating times. Described here is a case of Pott's disease of the thoracolumbar spine and how intraoperative image guidance can facilitate operative progress and accuracy in a patient in whom the underlying disease has severely deformed the normal anatomy of the spine. Added confidence about the location of vital structures as the surgeon proceeds with resection of the vertebral bodies and discs is depicted. Facilitation with image-guided placement of bicortical vertebral body screws and an interbody device is demonstrated. A diagram of the recommended positioning of the equipment in the operating room is provided along with “pearls” learned from our experience with this application. We believe that even the most experienced and skilled surgeon will find facilitation of anterior thoracolumbar surgery with image guidance to be of considerable benefit.
Endoscopic Aqueductal Stenting via the Fourth Ventricle under Navigating System Guidance: Technical Note
Authors: Hamada Hideo , Hayashi Nakamasa , Kurimoto Masanori , Endo Shunro 
Published: February 2005

AbstractOBJECTIVE:We report three patients with symptomatic isolated fourth ventricle after ventriculoperitoneal shunt placement for hydrocephalus associated with ventricular hemorrhage. All three patients were treated successfully with our new method of endoscopic aqueductal stenting under navigating system guidance.METHODS:A therapeutic rigid endoscope was inserted through the thin cerebellar hemisphere, and endoscopic aqueductal stenting was performed via the enlarged fourth ventricle under navigating system guidance.RESULTS:All three patients underwent successful procedures with good outcomes.CONCLUSION:Our method of aqueductal stenting is a reasonable choice for initial treatment of patients with isolated fourth ventricle, and it entails less invasive neurosurgery.
Techniques for the Ventral Correction of Postsurgical Cervical Kyphotic Deformity
Authors: Stewart Todd J. , Steinmetz Michael P. , Benzel Edward C. 
Published: February 2005

Abstract
PROGRESSIVE KYPHOTIC DEFORMITY of the cervical spine may be a late sequela of congenital cervical anomalies, degenerative disc disease, neoplasm, cervical trauma, and surgical procedures. Of these, postsurgical kyphosis is the most common and can occur after ventral and dorsal surgeries of the cervical spine. The purpose of this article is to review the causes and clinical presentation of postsurgical cervical kyphosis and to focus on the operative planning and ventral correction techniques.
Anatomic and Surgical Basis of the Sphenoid Ridge Keyhole Approach for Cerebral Aneurysms
Authors: Nathal Edgar , Gomez-Amador Juan Luis 
Published: February 2005

Abstract
IN VASCULAR NEUROSURGERY, the pterional approach has been used primarily for the treatment of a wide variety of diseases (cavernous angiomas, arteriovenous malformations, etc.), and it is used to take advantage of naturally occurring planes and spaces to expose the major structures of the circle of Willis. It provides access to the major part of the anterior circulation aneurysms and those occurring in the upper and most proximal part of the posterior circulation. Conversely, there has been an increasing interest in the so-called minimally invasive procedures or keyhole approaches to treating cerebral aneurysms in specific locations. In this work, we describe a novel keyhole approach that was conceived to achieve the angle of vision and advantages of the classic pterional approach. This surgical approach is based on the anatomic location of the sphenoid ridge and its relationship with the sylvian fissure and basal cisterns. The initial incision is made over the hairline behind the external border of the eye on the side selected. A skin and muscular flap is reflected anteriorly, and a small 3 × 3-cm craniotomy is completed around the external landmarks of the sphenoid ridge. Further extradural drilling is completed down to the anterior clinoid process. The dura is opened in a semilunar manner, and the sylvian fissure is opened completely to reach the sylvian and basal cisterns. Thereafter, the aneurysm is dissected and clipped according to the standard microtechnique of the neurosurgeon. A step-by-step description of the approach is offered in this work to facilitate a clear understanding of it. We recommend this approach for treatment of aneurysms arising at the anterior part of the circle of Willis. It has the advantages of less operative time, fewer days of hospitalization, and similar morbidity and mortality compared with the standard pterional craniotomy (5.7% on our service for nongiant ruptured aneurysms).
Cortical Surface Tracking Using a Stereoscopic Operating Microscope
Authors: Sun Hai , Roberts David W. , Farid Hany , Wu Ziji , Hartov Alex , Paulsen Keith D. 
Published: February 2005

AbstractOBJECTIVE:To measure and compensate for soft tissue deformation during image-guided neurosurgery, we have developed a novel approach to estimate the three-dimensional (3-D) topology of the cortical surface and track its motion over time.METHODS:We use stereopsis to estimate the 3-D cortical topology during neurosurgical procedures. To facilitate this process, two charge-coupled device cameras have been attached to the binocular optics of a stereoscopic operating microscope. Before surgery, this stereo imaging system is calibrated to obtain the extrinsic and intrinsic camera parameters. During surgery, the 3-D shape of the cortical surface is automatically estimated from a stereo pair of images and registered to the preoperative image volume to provide navigational guidance. This estimation requires robust matching of features between the images, which, when combined with the camera calibration, yields the desired 3-D coordinates. After the 3-D cortical surface has been estimated from stereo pairs, its motion is tracked by comparing the current surface with its previous locations.RESULTS:We are able to estimate the 3-D topology of the cortical surface with an average error of less than 1.2 mm. Executing on a 1.1-GHz Pentium machine, the 3-D estimation from a stereo pair of 1024 × 768 resolution images requires approximately 60 seconds of computation. By applying stereopsis over time, we are able to track the motion of the cortical surface, including the pulsatile movement of the cortical surface, gravitational sag, tissue bulge as a result of increased intracranial pressure, and the parenchymal shape changes associated with tissue resection. The results from 10 surgical patients are reported.CONCLUSION:We have demonstrated that a stereo vision system coupled to the operating microscope can be used to efficiently estimate the dynamic topology of the cortical surface during surgery. The 3-D surface can be coregistered to the preoperative image volume. This unique intraoperative imaging technique expands the capability of the current navigational system in the operating room and increases the accuracy of anatomic correspondence with preoperative images through compensation for brain deformation.
Surgical Technique of Lumbar Artificial Disc Replacement with the Charité Artificial Disc
Authors: Geisler Fred H. 
Published: February 2005

AbstractLUMBAR ARTIFICIAL DISC technology has been commercially available outside the United States for nearly 2 decades. With the Food and Drug Administration approval of the Charité Artificial Disc in October 2004, an entirely new spinal surgeon population will be able to offer this technology to their patients as a treatment option. As with other techniques in spinal surgery, indications for lumbar total disc replacement are paramount to the success of the procedure. The correct surgical technique is also important to a successful outcome. This article describes the technique for placement of the Charité Artificial Disc in indicated patients. The technique is similar to that of an anterior lumbar interbody fusion procedure, but many differences between the techniques make lumbar total disc replacement a unique procedure in the spinal surgeon's armamentarium. Although this article is thorough in its description of the surgical technique for total disc replacement with the Charité Artificial Disc, it should not be used as a substitute for company-sponsored training.
Cervical Arthroplasty with the Bryan Disc
Authors: Wang Michael Y. , Leung Clarence H.S. , Casey Adrian T.H. 
Published: February 2005

Abstract
CERVICAL ARTHROPLASTY IS a promising nonfusion alternative for the treatment of degenerative disc disease. After anterior cervical discectomy for neurological decompression, the intervertebral space is reconstructed by use of a metal and polymer prosthesis, allowing semiconstrained motion in multiple planes. This approach allows for preservation of cervical motion, potentially reducing the risk of transitional-level disease.
Her odours make him deaf: crossmodal modulation of olfaction and hearing in a male moth
Authors: Skals Niels , Anderson Peter , Kanneworff Morten , Löfstedt Christer , Surlykke Annemarie 
Published: February 2005

SUMMARYAll animals have to cope with sensory conflicts arising from simultaneous input of incongruent data to different sensory modalities. Nocturnal activity in moths includes mate-finding behaviour by odour detection and bat predator avoidance by acoustic detection. We studied male moths that were simultaneously exposed to female sex pheromones indicating the presence of a potential mate, and artificial bat cries simulating a predation risk. We show that stimulation of one sensory modality can modulate the response to information from another, suggesting that behavioural thresholds are dynamic and depend on the behavioural context. The tendency to respond to bat sounds decreased as the quality and/or the amount of sex pheromone increased. The behavioural threshold for artificial bat cries increased by up to 40 dB when male moths where simultaneously exposed to female sex pheromones. As a consequence, a male moth that has detected the pheromone plume from a female will not try to evade an approaching bat until the bat gets close, hence incurring increased predation risk. Our results suggest that male moths'reaction to sensory conflicts is a trade-off depending on the relative intensity of the input to CNS from the two sensory modalities.
Odor-modulated orientation in walking male cockroachesPeriplaneta americana, and the effects of odor plumes of different structure
Authors: Willis M. A. , Avondet J. L. 
Published: February 2005

SUMMARYThe location of distant resources using odor information usually also requires information on the flow of air (or water) through the environment together with the expression of internally programmed steering responses. The orientation responses of virgin male Periplaneta americana L. to wind and the female sex-pheromone component (-)-periplanone-B were video-recorded in a laboratory wind tunnel and quantified. P. americana males showed no preferred walking orientation when released in zero wind in the absence of pheromone. When introduced into 25 cm s-1 wind in the absence of pheromone, 79% of males walked downwind. Upon introduction to a plume of(-)-periplanone-B in wind, 100% of males walked upwind in the pheromone plume to the source. Males were then challenged with wind-borne plumes of(-)-periplanone-B of four different temporal/spatial structures. In nearly all cases, the only statistically significant changes in behavioral parameters measured from their walking tracks were observed from males tracking the treatment consisting of the most turbulent plume. The plume-tracking performances of males challenged with the other three less turbulent plumes were visually and quantitatively similar, regardless of the width. Males tracking all four plumes showed evidence of turns resulting from multiple mechanisms. Some of the observed maneuvers were temporally regular counterturns, suggesting steering according to an ongoing internal program,while others could have been triggered by encountering the change in odor concentration between pheromone and clean air at the lateral boundary of the plume or chemotactically upon the detection of changes in odor concentration.
Sites and modes of action of proctolin and the FLP F2 on lobster cardiac muscle
Authors: Wilkens J. L. , Shinozaki T. , Yazawa T. , ter Keurs H. E. D. J. 
Published: February 2005

SUMMARYAt the threshold concentration (1-10 pmol l-1), the neuropeptide hormones proctolin (PR) and the FLRFamide-like peptide (FLP) F2cause an increase in amplitude of electrically evoked contractions (each contraction is a brief tetanus) of lobster heart ostial muscle. At higher concentrations each peptide also induces an increase in tonus (contracture). The PR-induced contracture and augmentation of tetani are proportional to increases in [Ca2+]i. The rate of onset and recovery of peptide-induced effects on both tetani and contracture appeared to reduced by Ca2+ storage by the sarcoplasmic reticulum (SR). Enhanced tetani following a contracture may be due to enhanced voltage-gated Ca2+current and sarcoplasmic reticular (SR) Ca2+ loading. The SR Ca2+ loading appears to be specific for PR and F2, since glutamic-acid-induced contractures are not followed by increased tetani. The prolonged elevation of [Ca2+]i during contracture causes a right-ward shift in the force-pCa curve indicating a decrease in myofibrillar sensitivity to Ca2+. Blocking voltage-gated Ca2+ channels with Cd2+, nifedipine or verapamil, while reducing tetani, does not prevent peptide-induced contracture and enhanced tetani. Opening SR Ca2+ channels and depleting SR Ca2+with either caffeine or ryanodine blocked tetani but permitted accelerated peptide-induced contractures. We conclude that PR and F2 at low concentration enhance voltage-dependent Ca2+ induced Ca2+ release from the SR, while higher hormone levels directly gate Ca2+ entry across the sarcolemma.