bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenetic analysis of Aedes species distributed in Taif Province, Saudi Arabia, based on mitochondrial COX1 sequences
Authors: Al Zahrani Mohammed R. , Al Ghamdi Khalid M. , Aljameeli Mohammad M. , Bakr Mohamed N. , Alghamdi Tariq S. , Haider Touseef Amna , Gharsan Fatehia N. , Alghamdi Samia Q. , Abdella Ehab M. , Mahyoub Jazem A. 
Published: December 2023

AbstractAs Aedes mosquitoes are the predominant carriers of arboviruses responsible for global dengue fever and chikungunya outbreaks, understanding their genetic diversity and population structure can enhance dengue prevention and vector control efforts. Although sympatric cryptic species have been acknowledged in Aedes species subgroups in Southeast Asia, little information is available regarding the occurrence and dispersal of cryptic Aedes species in Saudi Arabia. In the present investigation, we intend to analyze genetic variations and perform a phylogenetic study of sympatric Aedes samples collected from various locations in Taif Province, Saudi Arabia. The locus of mitochondrial DNA cytochrome c oxidase subunit 1 (COX1, analyzed with DNA barcoding) was utilized to determine genetic variance and to build phylogenetic trees. For species identification, the COX1 sequences of Aedes samples from Taif Province were compared with those of Aedes samples from GenBank (National Center for Biotechnology Information, NCBI). To identify phylogenetic relationships and genetic variability, phylogenetic trees were created using Taif Province samples, GenBank A. albopictus samples, and GenBank A. aegypti samples. The phylogenetic analysis revealed that some Taif samples (A3, A7, F2, F4, F1, A4, A1, F9 and F6) were closer to A. aegypti and A. albopictus samples from Africa and East Asia, especially Kenya and Malaysia. Whereas other samples (F9, F6, A4, F1 and A1) were closer to the GenBank A. aegypti samples KP843383 (Thailand), HM399357(Australia), MK533632 (Kenya), KX420460 (Kenya), KU495081 (Australia) and MF179160 (China) than the remaining Taif samples. Overall, our findings demonstrate that examining the genetic diversity and phylogenetic linkages of Aedes samples can aid in understanding the evolution of Aedes cryptic species in the western regions of Saudi Arabia.
A minimally invasive, field‐applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white‐nose syndrome in bats
Authors: Pérez Adam A. , Tobin Abigail , Stechly John V. , Ferrante Jason A. , Hunter Margaret E. 
Published: December 2023

AbstractThe accessibility to CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR‐associated protein) genetic tools has given rise to applications beyond site‐directed genome editing for the detection of DNA and RNA. These tools include precise diagnostic detection of human disease pathogens, such as SARS‐CoV‐2 and Zika virus. Despite the technology being rapid and cost‐effective, the use of CRISPR/Cas tools in the surveillance of the causative agents of wildlife diseases has not been prominent. This study presents the development of a minimally invasive, field‐applicable and user‐friendly CRISPR/Cas‐based biosensor for the detection of Pseudogymnoascus destructans (Pd), the causative fungal agent of white‐nose syndrome (WNS), an infectious disease that has killed more than five million bats in North America since its discovery in 2006. The biosensor assay combines a recombinase polymerase amplification (RPA) step followed by CRISPR/Cas12a nuclease cleavage to detect Pd DNA from bat dermal swab and guano samples. The biosensor had similar detection results when compared to quantitative PCR in distinguishing Pd‐positive versus negative field samples. Although bat dermal swabs could be analysed with the biosensor without nucleic acid extraction, DNA extraction was needed when screening guano samples to overcome inhibitors. This assay can be applied to help with more rapid delineation of Pd‐positive sites in the field to inform management decisions. With further optimization, this technology has broad translation potential to wildlife disease‐associated pathogen detection and monitoring applications.
Petrogenesis of Oligocene Granitoids and its Implications on Beryllium Mineralization at Pagele, Nianqingtanggula Mountain (Central Tibet)
Authors: ZHANG Linyuan , YANG Zhusen , HOU Zengqian , WU Changda , LIU Chang , XU Peiyan , XIE Lankui 
Published: December 2023

AbstractThe newly discovered Oligocene granitoids (33.1–28.7 Ma) at Pagele are magmatic rocks related to beryllium mineralization during the India–Asia late‐collisional stage. This discovery provides an ideal example to study the late‐collisional orogeny and beryllium prospecting in the Lhasa terrane. The Oligocene granitoids include porphyritic granodiorite, Stage I, II and III granites, and granitic pegmatite. Geochemical analysis shows that the porphyritic granodiorite is characterized by high SiO2, K2O, total ΣREE contents, and (La/Yb)N ratios; while the latter two by higher SiO2, lower ΣREE and (La/Yb)N ratios. Notably, the granitic pegmatite has extremely high Y/Ho, low K/Rb and Zr/Hf, and distinct REE tetrad effect (1.14–1.21). This study suggests that the porphyritic granodiorite may be derived from partial melting of beryllium‐rich materials composed of Lhasa ancient crust (70%–80%) and enriched Lhasa lithospheric mantle (20%–30%) under the tearing subduction of Indian slab. The three‐stage granites and granitic pegmatite, which contain higher beryllium contents or beryls, were likely generated by highly fractionation of the porphyritic granodioritic magma or other homologous magma. Considering the possible genetic and spatial link between Indian slab tear and rifts, we suggest that highly‐fractionated granites in rifting systems represent important Be prospecting targets in the Lhasa terrane.
A Real‐time Lithological Identification Method based on SMOTE‐Tomek and ICSA Optimization
Authors: DENG Song , PAN Haoyu , LI Chaowei , YAN Xiaopeng , WANG Jiangshuai , SHI Lin , PEI Chunyu , CAI Meng 
Published: December 2023

AbstractIn petroleum engineering, real‐time lithology identification is very important for reservoir evaluation, drilling decisions and petroleum geological exploration. A lithology identification method while drilling based on machine learning and mud logging data is studied in this paper. This method can effectively utilize downhole parameters collected in realtime during drilling, to identify lithology in real‐time and provide a reference for optimization of drilling parameters. Given the imbalance of lithology samples, the synthetic minority over‐sampling technique (SMOTE) and Tomek link were used to balance the sample number of five lithologies. Meanwhile, this paper introduces Tent map, random opposition‐based learning and dynamic perceived probability to the original crow search algorithm (CSA), and establishes an improved crow search algorithm (ICSA). In this paper, ICSA is used to optimize the hyperparameter combination of random forest (RF), extremely random trees (ET), extreme gradient boosting (XGB), and light gradient boosting machine (LGBM) models. In addition, this study combines the recognition advantages of the four models. The accuracy of lithology identification by the weighted average probability model reaches 0.877. The study of this paper realizes high‐precision real‐time lithology identification method, which can provide lithology reference for the drilling process.
Magmatic‐hydrothermal Evolution and Mineralization Mechanisms of the Wangjiazhuang Cu (‐Mo) Deposit in the Zouping Volcanic Basin, Shandong Province, China: Constraints from Fluid Inclusions
Authors: SHU Lei , YANG Renchao , SHEN Kun , YANG Deping , MAO Guangzhou , LI Min , LIU Pengrui , MA Xiaodong 
Published: December 2023

AbstractThe Wangjiazhuang Cu (‐Mo) deposit, located within the Zouping volcanic basin in western Shandong Province, China, is unique in this area for having an economic value. In order to expound the metallogenetic characteristics of this porphyry‐like hydrothermal deposit, a detailed fluid inclusion study has been conducted, employing the techniques of representative sampling, fluid inclusion petrography, microthermometry, Raman spectroscopy, LA‐ICP‐MS analysis of single fluid inclusions, as well as cathode fluorescence spectrometer analysis of host mineral quartz. The deposit contains mainly two types of orebodies, i.e. veinlet‐dissemination‐stockwork orebodies in the K‐Si alteration zone and pegmatitic‐quartz sulfide veins above them. In addition, minor breccia ore occurs locally. Four types of fluid inclusions in the deposit and altered quartz monzonite are identified: L‐type one‐ or two‐phase aqueous inclusions, V‐type vapor‐rich inclusions with V/L ratios greater than 50%–90%, D‐type multiphase fluid inclusions containing daughter minerals or solids and S‐type silicate‐bearing fluid inclusions containing mainly muscovite and biotite. Ore petrography and fluid inclusion study has revealed a three‐stage mineralization process, driven by magmatic‐hydrothermal fluid activity, as follows. Initially, a hydrothermal fluid, separated from the parent magma, infiltrated into the quartz monzonite, resulting in its extensive K‐Si alteration, as indicated by silicate‐bearing fluid inclusions trapped in altered quartz monzonite. This is followed by the early mineralization, the formation of quartz veinlets and dissemination‐stockwork ores. During the main mineralization stage, due to the participation and mixing of meteoric groundwater with magmatic‐sourced hydrothermal fluid, the cooling and phase separation caused deposition of metals from the hydrothermal fluids. As a result, the pegmatitic‐quartz sulfide‐vein ores formed. In the late mineralization stage, decreasing fluid salinity led to the formation of L‐type aqueous inclusions and chalcopyrite‐sulfosalt ore. Coexistence of V‐type and D‐type inclusions and their similar homogenization temperatures with different homogenization modes suggest that phase separation or boiling of the ore‐forming fluids took place during the early and the main mineralization stages. The formation P‐T conditions of S‐type inclusions and the early and the main mineralization stages were estimated as ca. 156–182 MPa and 450–650°C, 350–450°C, 18–35 MPa and 280–380°C, 8–15 MPa, respectively, based on the microthermometric data of the fluid inclusions formed at the individual stages.
Conserved infections and reproductive phenotypes of Wolbachia symbionts in Asian tortrix moths
Authors: Arai Hiroshi , Ueda Masatoshi , Hirano Tatsuya , Akizuki Naoya , Lin Shiou‐Ruei , Hanh Duong Kieu , Widada Jaka , Rohman Muhammad Saifur , Nakai Madoka , Kunimi Yasuhisa , Vang Le Van , Wijonarko Arman , Inoue Maki N. 
Published: December 2023

AbstractWolbachia is a ubiquitous endosymbiotic bacterium that manipulates insect reproduction. A notable feature of Wolbachia is male killing (MK), whereby sons of infected females are killed during development; however, the evolutionary processes by which Wolbachia acquired the MK ability remain unclear. The tea tortrix moth Homona magnanima (Tortricidae) harbours three non‐MK Wolbachia strains (wHm‐a, wHm‐b and wHm‐c) and an MK strain wHm‐t. Although wHm‐t and wHm‐c are closely related, only wHm‐t has an MK‐associated prophage region. To understand the evolutionary processes underlying the emergence of MK wHm‐t, we examined Wolbachia infections and phenotypes in 62 tortricid species collected from 39 localities across Japan, Taiwan, Vietnam and Indonesia. PCR assays detected wHm‐c relatives in 51 species and triple infection of wHm‐a, wHm‐b and wHm‐c in 31 species. Apart from Taiwanese H. magnanima, no species exhibited the MK phenotype and were positive for the wHm‐t‐specific prophage. While wHm‐t infection was dominant in Taiwanese H. magnanima, wHm‐a, wHm‐b and wHm‐c were dominant in Japanese H. magnanima populations. These results suggest that wHm‐a, wHm‐b and wHm‐c strains descended from a common ancestor with repeated infection loss and that wHm‐t evolved from the wHm‐c acquiring MK ability in allopatric populations of H. magnanima.
Relationship between the severity of emphysematous change in the lung and morbidity after esophagectomy for esophageal cancer: A retrospective study on a novel strategy for risk prediction
Authors: Horinouchi Tomo , Yoshida Naoya , Shiraishi Shinya , Hara Yoshihiro , Matsumoto Chihiro , Toihata Tasuku , Kosumi Keisuke , Harada Kazuto , Eto Kojiro , Ogawa Katsuhiro , Sawayama Hiroshi , Iwatsuki Masaaki , Baba Yoshifumi , Miyamoto Yuji , Baba Hideo 
Published: December 2023

AbstractBackgroundChronic obstructive pulmonary disease (COPD) prevalence increases post‐esophagectomy morbidity. However, the association between COPD severity and post‐esophagectomy morbidity remains unclear because of the lack of an objective method to classify COPD severity. Low attenuation volume ratio (LAVR) estimated using Ziostation2 may reflect the extent of emphysematous changes in the lungs and COPD severity, thereby predicting post‐esophagectomy morbidity.MethodsA total of 776 patients who underwent curative McKeown esophagectomy for esophageal cancer between April 2005 and June 2021 were included. The patients were divided into high and low preoperative LAVR groups. Short‐term outcomes between the groups were compared for patients who underwent open esophagectomy (OE) and minimally invasive esophagectomy (MIE).ResultsA total of 219 (28%) patients were classified into the high LAVR group. High LAVR was significantly associated with disadvantageous patient characteristics such as advanced age, heavy smoking, and impaired respiratory function. Patients with high LAVR had a significantly higher incidence of severe morbidity and pneumonia after OE. High LAVR was an independent risk factor for severe morbidity (odds ratio [OR], 2.52; 95% confidence interval [CI]: 1.237–5.143; p = 0.011) and pneumonia (OR, 2.12; 95% CI: 1.003–4.493; p = 0.049) after OE. Meanwhile, LAVR was not correlated with the incidence of post‐MIE morbidity.ConclusionsLAVR may reflect COPD severity and predict severe morbidity and pneumonia after OE, but not after MIE. Less invasiveness of MIE may alleviate the effects of various disadvantageous backgrounds associated with high LAVR on worse short‐term outcomes.
Cardiopulmonary bypass management and acute kidney injury in cardiac surgery patients
Authors: Barbu Mikael , Hjärpe Anders , Martinsson Andreas , Dellgren Göran , Ricksten Sven‐Erik , Lannemyr Lukas , Pivodic Aldina , Taha Amar , Jeppsson Anders 
Published: December 2023

AbstractBackgroundCardiopulmonary bypass (CPB) ensures tissue oxygenation during cardiac surgery. New technology allows continuous registration of CPB variables during the operation. The aim of the present investigation was to study the association between CPB management and the risk of postoperative acute kidney injury (AKI).MethodsThis observational study based on prospectively registered data included 2661 coronary artery bypass grafting and/or valve patients operated during 2016–2020. Individual patient characteristics and postoperative outcomes collected from the SWEDEHEART registry were merged with CPB variables automatically registered every 20 s during CPB. Associations between CPB variables and AKI were analyzed with multivariable logistic regression models adjusted for patient characteristics.ResultsIn total, 387 patients (14.5%) developed postoperative AKI. After adjustments, longer time on CPB and aortic cross‐clamp, periods of compromised blood flow during aortic cross‐clamp time, and lower nadir hematocrit were associated with the risk of AKI, while mean blood flow, bladder temperature, central venous pressure, and mixed venous oxygen saturation were not. Patient characteristics independently associated with AKI were advanced age, higher body mass index, hypertension, diabetes mellitus, atrial fibrillation, lower left ventricular ejection fraction, estimated glomerular filtration rate <60 or >90 mL/min/m2, and preoperative hemoglobin concentration below or above the normal sex‐specific range.ConclusionsTo reduce the risk of AKI after cardiac surgery, aortic clamp time and CPB time should be kept short, and low hematocrit and periods of compromised blood flow during aortic cross‐clamp time should be avoided if possible.
Edaphic conditions affect plant life‐history trait syndromes in a tropical ancient biodiverse ecosystem
Authors: Gomes Vanessa M. , Renton Michael , Riviera Fiamma , Hobbs Richard J. , Aguilar Ramiro , Fernandes Geraldo Wilson 
Published: December 2023

AbstractA functional approach to understand natural plant communities helps elucidate the dynamics of environmental filters and community assembly. Species classification into functional groups allows the translation of ecological complexity into simplified and applicable knowledge. Here, we developed a plant functional type (PFT) system to investigate whether functional structure (PFT composition and abundance) of plant communities varies between substrate types and plant physiognomies in an old climatically buffered infertile landscape (OCBIL), namely Brazilian campo rupestre. We sampled the herbaceous and shrubby vegetation and collected soil variables in four different plant physiognomies growing in quartzitic and ferruginous substrates. We classified the sampled species according to life‐history traits related to species persistence, reproduction, and biological interactions. Functional dissimilarity among plant physiognomies and the two substrate types and the role of soil factors affecting the dissimilarities were assessed. Pollination and seed dispersal syndromes, mycorrhizal association, and reproductive phenology were the main traits defining the PFTs. We found distinct functional structure between plant communities of different substrates and physiognomies, with a marked influence of chemical and physical soil factors. Also, we defined main PFTs profiles for each physiognomy, identifying core PFTs with basic combinations of traits, common to all or almost all physiognomies. The functional perspective proposed by this study provides applicable knowledge to the restoration and conservation of campo rupestre ecosystem, reinforcing the importance of habitat heterogeneity for maintaining its functioning.
Did the 2021 federal change in the practice guidelines for the administration of buprenorphine for treating opioid use disorder increase buprenorphine prescription dispensing in Medicaid population?
Authors: Qian Jingjing , Khatiwada Asmita P. , Xue Xiangzhong 
Published: December 2023

AbstractBackground and ObjectivesIn April 2021, the Department of Health and Human Services released new federal practice guidelines and allowed physicians who wish to treat ≤30 patients with opioid use disorder (OUD) to forego the X‐waiver training requirement.MethodsThis observational study compared annual number, dose, and spending of buprenorphine OUD treatments dispensed in the Medicaid population in 2021 versus 2020 using the CMS State Drug Utilization Data (n = 50 states plus D.C.).ResultsCompared to 2020, there was a slight decrease (−3.1%) in the annual number of buprenorphine prescriptions dispensed but an increase in total doses (+3.2%) and payment (10.6%) for buprenorphine prescriptions in 2021.Discussion and ConclusionsDecrease in number of buprenorphine prescriptions in Medicaid population was observed in 2021.Scientific SignificanceOur findings support the hypothesis generation in which the removal of X‐waiver training alone is not adequate to increase prescribing and access to OUD treatment buprenorphine.
Calcinosis universalis accompanied by symptomatic systemic sclerosis
Authors: Geng Shiling , Zhang Yihua , Gong Ting , Ji Chao 
Published: December 2023

AbstractCalcinosis cutis is represented by the deposition of insoluble calcium salts in the skin and subcutaneous tissue. Calcinosis can lead to repeated episodes of local inflammation and repeated infections, resulting in pain and functional disability, and even death. Here, we present a case of a patient with SSc who experienced calcinosis universalis and eventually died from recurrent infections at the sacrococcygeal calcification.
Intravitreal anti‐vascular endothelial growth factor therapy for retinal diseases in Norway from 2011 to 2021: A combined registry and survey study
Authors: Husum Yngvil Solheim , Bråten Ragnhild Haugli , Sæther Erik Magnus , Moe Morten Carstens , Kristiansen Ivar Sønbø , Jørstad Øystein Kalsnes 
Published: December 2023

AbstractPurposeTo investigate the use of intravitreal anti‐vascular endothelial growth factor (anti‐VEGF) therapy in Norway from 2011 to 2021 and explore how the eye departments organized their injection services.MethodsWe combined data from the Norwegian Patient Registry (NPR) with survey responses from Norway's 22 eye departments. The NPR data encompassed all registered intravitreal injection episodes from 2011 to 2021. The survey contained questions about local treatment practices and emphasized neovascular age‐related macular degeneration (nAMD), retinal vein occlusion and diabetic macular edema.ResultsA total of 47247 unique patients received 841 646 intravitreal injections in the study period. The number of patients per year increased from 6522 in 2011 to 20 635 in 2021. The number of injections per year increased from 30 926 in 2011 to 125 258 in 2021. The most frequent diagnosis was nAMD. In 2021, the age‐adjusted treatment activity in Norway's 11 counties ranged from 47.8 to 75.5 injections per 1000 inhabitants aged ≥50 years. The use of aflibercept gradually exceeded bevacizumab, but the aflibercept proportion per county ranged from 38 to 82% in 2021. The survey revealed varying treatment practices, local guidelines were often absent, and only half of the departments defined a lower visual limit for initiating or maintaining treatment.ConclusionThe use of intravitreal anti‐VEGF therapy increased considerably from 2011 to 2021, but there was considerable regional variation in treatment activity, drug utilization and organization of injection services. These findings emphasize the need for strengthened governance and national guidelines to ensure equal treatment nationally.