bluuebunny/crossref_metadata_2025_split huggingface dataset:
One-Step Fabrication of a Self-Driven Point-of-Care Device by Femtosecond Laser Direct Writing and Its Application in Cancer Cell H2O2 Detection via Semiconductor-Based SERS
Authors: Yu Zhi , Wang Yue , Lai Bo , Xu Zhangrun 
Published: October 2023

Abstract
Self-driven microfluidic systems have attracted significant attention and demonstrated great potential in the field of point-of-care (POC) testing due to their device simplicity, low power consumption, increased portability, and reduced sample consumption. To develop POC detection devices with diverse characteristics that meet different requirements, there is a strong demand for feasible strategies that enable easy operation and reduce processing time. Here, we proposed a one-step processing approach using femtosecond laser direct writing technology to fabricate a capillary-actuated POC microfluidic chip. The driving force of the chip is highly dependent on its surface wettability, which can be easily adjusted by changing the laser processing parameters. This POC microfluidic chip allowed for the detection of intracellular H2O2 through a catalytic reaction system that incorporated 5-aminosalicylic acid (5-Asa)-sensitized colloidal TiO2 nanoparticles and horse radish peroxidase (HRP), combined with the integration of semiconductor-based surface-enhanced Raman scattering (SERS) spectroscopy. The concentration of H2O2 was determined by the SERS signal of the catalytic products, resulting in rapid detection with minimal sample consumption. Our method provides a simple, feasible, and alternative strategy for fabricating self-driven POC devices, which was successfully applied to the rapid detection of intracellular H2O2 in MCF-7 breast cancer cells with high sensitivity and tiny sample consumption. This work not only demonstrates the exceptional advantages of femtosecond laser processing technology in fabricating diverse microfluidic devices for various applications, but also presents an efficient POC testing strategy for detecting cell signaling molecules and enabling early diagnosis of breast cancer.
In vitro mass propagation of Dendrocalamus asper (Giant bamboo) through direct organogenesis
Authors: Gunasena Kasundi , Chandrasena Priyantha , Senarath Sandun 
Published: October 2023

Abstract

Dendrocalamus asper (giant bamboo) is a clumping type bamboo belonging to the family Poaceae. With its economic and environmental value, demand for this species has increased tremendously. Conventional propagation methods have limitations due to low seed viability and unavailability of healthy clumps. Therefore, an in vitro mass propagation protocol was developed to provide healthy plants for large scale plantations. Nodal segments were used for shoot initiation and the best medium for shoot induction, best medium for multiple shoot induction, effect of shoot cluster size and effect of physical state of the medium on multiple shoot induction were determined. Elongated shoots were transferred into rooting medium and best medium for root induction and effect of cluster size on rooting were determined. Well-developed plantlets were transferred to coir pellets and after four weeks transferred into different potting mixtures. According to the results, highest mean number of shoots per node (16.87 ± 0.52), mean shoot length (4.12 ± 0.27 cm) and mean number of leaves per shoot (4.80 ± 0.33) were observed in MS medium supplemented with 1.0 mg/L BAP. MS medium supplemented with 2.0 mg/L BAP was the best for multiple shoot induction, shoot clusters with 3 shoots was the best cluster size and liquid medium had better effect on shoot multiplication. Half strength MS medium supplemented with 2.0 mg/L IBA was the best for in vitro root induction with highest mean number of roots (7.15 ± 0.77) and mean root length (10.79 ± 1.11cm). Shoot clusters with 3 shoots was the best cluster size for root induction. Sand: compost: coir dust (1:1:1) mixture was the best potting mixture giving 100% survival. These findings provide a reliable micropropagation protocol for D. asper, which holds great promise for meeting the growing demand for bamboo resources and promoting sustainable bamboo cultivation.
Extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Guangzhou, China: risk factors and economic burden among Patients with bloodstream infections
Authors: Chen Jiakang , Allel Kasim , Luo Wenwei , He Nanhao , Yang Xu , Zhuo Chuyue , Guo Yingyi , Wang Jiong , Yao Likang , Li Jiahui , Lin Yexin , Tu Ruiyang , Yakob Laith , Zhuo Chao 
Published: October 2023

Abstract
Background.
Extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EK) are important sources of bloodstream infection (BSI), but their economic impact has not been comprehensively evaluated.
Patients and methods. A
retrospective analysis of inpatients diagnosed with Escherichia coli or Klebsiella pneumoniae bacteremia in tertiary hospitals between January 2020 and December 2022 was performed. Clinical data and healthcare spending were collected through a chart review of electronic medical records. The chi-square test was used to analyze risk factors for ESBL, and then propensity score matching (PSM) was used to match patients 1:1 ratio to eliminate confounding factors and economic burden was assessed using disability-adjusted life years (DALYs).
Results.
A total of 318 patients meeting study specifications were identified. Before matching, there were statistically significant differences in hospitalization time before infection, organ transplantation rate, ICU admission rate, Age-adjusted Charlson Comorbidity Index (ACCI), and SOFA score between the two groups (all p<0.05). After using PSM-adjusted estimates, the ESBL group still had a lower rate of effective empirical antimicrobial therapythan the non-ESBL group (non-ESBL=79.3% vs ESBL=59.5% p=0.001). The ESBL group had a higher hospitalization cost, but there was no significant difference in total mortality (non-ESBL=19.0% vs ESBL=20.7%, p=0.747) and mean DALYs (non-ESBL=1.84 DALYs vs ESBL=2.12 DALYs, p=0.098) between two groups. Mediation analysis identified that the relationship between ESBL and hospitalization costs is largely or fully mediated by inappropriate empirical antibiotic therapy and hospital stay.
Conclusion.
The cost of patients with BSI caused by ESBL-EK is higher than that of patients with BSI caused by non-ESBL-EK. This phenomenon can be attributed to differences in effective empirical antimicrobial therapy rates and length of hospitalization. Reasonable assessment of ESBL risk，fully consider the regional epidemiological situation and precise use of antibiotics are very important to reduce the burden on patients.
Mutation Spectrum analysis of ITGA3 gene associated with Nephrotic syndrome
Authors: Nazir QuratulAin , Shehzad Wasim , Ali Muhammad Asad , Zahoor Muhammad Yasir 
Published: October 2023

Abstract
Background Nephrotic syndrome is a renal disorder in which the Glomerular Filtration Barrier (GFB) is affected. There is a potential indication that ITGA3 may have a pivotal function in the intricate interaction between cells, morphogens, and the extracellular matrix (ECM) that is essential for the development of the kidneys (nephrogenesis). The present study involves a detailed analysis of the reported missense mutations in the ITGA3 gene through various in silico and bioinformatics tools. The data about reported mutations was collected from HGMD and tools such as PolyPhen-2, SIFT, I-Mutant were used to predict the affects of these mutations. The conservation analysis of this protein was also done by analyzing which mutations fall on the conserved region of the protein and are hence more detrimental.Results A total of 7 mutations were identified, out of which one (A349S) was found to have the least detrimental affect on the protein structure. The phylogenetic analysis of the ITGA protein family was also done to determine the relationship of ITGA3 protein with the other proteins in its family.Conclusion The data obtained in this study is aimed to facilitate future studies on ITGA3 protein and its role in the development of the Nephrotic syndrome, along with the implication of the mutations on the structure and function of the ITGA3 protein. This study also gives an insight on the detrimental effect of the mutations on the protein.
Rapid-mixing production of lipid nanoparticles for biomolecules delivery
Authors: CHIESA ENRICA , Caimi Alessandro , Bellotti Marco , Giglio Alessia , Conti Bice , Dorati Rossella , Auricchio Ferdinando , Genta Ida 
Published: October 2023

Abstract
During past years, lipid nanoparticles (LNPs) have emerged as helpful tool to deliver sensible biomolecules such as protein and oligonucleotides demonstrating high efficiency in vitro and in vivo. However, the translation of these nanosystems from bench to bedside has been limited mainly because the LNPs production methods are usually associated to poor control over the lipid’s assembly and hence LNPs quality attributes. In this framework, microfluidics offers the opportunity to overcome this issue since it allows fast, reproducible, and robust LNPs production and now it is revolutionizing the R&D sections since it is the ideal choice for a rapid prototyping of a formulation. New microfluidic platforms need to be developed and customized considering the production process. Within the present work, the feasibility of producing LNPs by a brand-new microfluidic platform was demonstrated by a systematic approach. Screening design of experiment and Box-Behnken design were applied to identify the operating parameters affecting the LNPs quality. The performances of the new production platform were assessed by producing neutral, stealth, and cationic LNPs and further, several model biomolecules were successfully loaded with a high encapsulation always higher than 70%.
Genetic Underpinnings of Social Trust: Insights from 33,882 Danish Blood Donors
Authors: Banasik Karina , Sequeros Celia Burgos , Hansen Thomas , Westergaard David , Louloudis Ioannis , Kalamajski Sebastian , Röder Timo , Rohde Palle Duun , Schwinn Michael , Clemmensen Line Harder , Didriksen Maria , Nyegaard Mette , Hjalgrim Henrik , Nielsen Kaspar , Bruun Mie Topholm , Ostrowski Sisse , Erikstrup Christian , Mikkelsen Susan , Sørensen Erik , Pedersen Ole , Brunak Søren , Giordano Giuseppe 
Published: October 2023

Abstract
Social trust is a heritable trait that has been linked with physical health and longevity. In this study, we performed genome-wide association studies of self-reported social trust in n=33,882 Danish blood donors. We observed genome-wide and local evidence of genetic similarity with other brain-related phenotypes, and estimated the single nucleotide polymorphism-based heritability of trust to be 15%. In our discovery cohort (n=25,819), we identified one significantly associated locus (lead variant: rs12776883) in an intronic enhancer region of PLPP4, a gene highly expressed in brain, kidneys, and testes. However, we could not replicate the signal in an independent set of donors who were phenotyped a year later (n=8,063). In the subsequent meta-analysis, we found a second significantly associated variant (rs71543507) in an intergenic enhancer region. Overall, our work confirms that social trust is heritable, and provides an initial look into the genetic factors that influence it.
Investigations on Ancient Bronze Drums from Majiang, Guangxi, P. R. China
Authors: Chao Zeng , Changzhong Liao , Fubin Wan , He Wei 
Published: October 2023

Abstract
The scientific and technological archaeological research of bronze drum is an important means to reveal the intrinsic scientific properties of bronze drums. In order to characterize the original scheme of polychromy and the used materials, six samples were collected from six different Majiang-type bronze drums from Hechi city of Guangxi province of China in Guangxi Museum. The composition of all the samples were determined by using scanning electron microscopy with Energy Dispersive X-ray spectroscopy. All the bronze drums contain mainly Cu, Sn, Pb and As. The X-ray diffraction analysis was carried out to analyze the component phases in the sample of the bronze drum. The Rietveld refinement was performed to do the quantitative phase analysis for the samples of the bronze drum (No. 0076 and No0093). The X-ray diffraction analysis and Rietveld structure refinement results show that No.0076 bronze drum contains four compounds: As0.04Cu0.96, Pb, Cu3Sn, and Cu10Sn3. Their mass percentage contents are 74.9 (4) wt.%, 5 (1) wt.%, 10 (1) wt.%, and 10 (1) wt.%, respectively. The No.0093 bronze drum contains five compounds Cu, Pb, Cu3Sn, As0.2Cu1.8, and Cu10Sn3, with mass percentages of 54.4 (5) wt.%, 5.3 (8) wt.%, and 9 (1) wt.%, 19 (1) wt.% and 12 (1) wt.%, respectively.
Mu-Suppression Neurofeedback Training Targeting the Mirror Neuron System: A Pilot Study
Authors: Dastgheib Samanehsadat , Wang Wenbo , Kaufmann Jürgen M. , Moratti Stephan , Schweinberger Stefan R. 
Published: October 2023

Abstract
Neurofeedback training (NFT) is a promising adjuvant intervention method. The desynchronization of mu rhythm (8–13 Hz) in the electroencephalogram (EEG) over centro-parietal areas is known as a valid indicator of mirror neuron system (MNS) activation, which has been associated with social skills. Still, the effect of neurofeedback training on the MNS requires to be well investigated. The present study examined the possible impact of NFT with a mu suppression training protocol encompassing 15 NFT sessions (45 min each) on 16 healthy neurotypical participants. In separate pre- and post-training sessions, 64-channel EEG was recorded while participants (1) observed videos with various types of movements (including complex goal-directed hand movements and social interaction scenes) and (2) performed the "Reading the Mind in the Eyes Test" (RMET). EEG source reconstruction analysis revealed statistically significant mu suppression during hand movement observation across MNS-attributed fronto-parietal areas after NFT. The frequency analysis showed no significant mu suppression after NFT, despite the fact that numerical mu suppression appeared to be visible in a majority of participants during goal-directed hand movement observation. At the behavioral level, RMET accuracy scores did not suggest an effect of NFT on the ability to interpret subtle emotional expressions, although RMET response times were reduced after NFT. In conclusion, the present study exhibited preliminary and partial evidence that mu suppression NFT can induce mu suppression in MNS-attributed areas. More powerful experimental designs and longer training may be necessary to induce substantial and consistent mu suppression, particularly while observing social scenarios.
Background Investigating the factors affecting the number of hospitalization days of people with diabetes and with covid_19, a cross sectional study
Authors: Allahyari Elahe , Boostani Amirhossein 
Published: October 2023

Abstract
Background
All over the world, the availability of hospital beds is one of the fundamental challenges of modern healthcare. On the other hand, the COVID-19 pandemic and underlying illnesses exacerbate this problem by increasing the number of hospitalization days. Therefore, this study aims to determine the factors that affect the number of days of hospitalization of people with diabetes and with COVID-19.
Method
The Artificial neural network models used information from 183 of 200 patients to train the multilayer feed-forward back-propagation algorithms in 70% of the data. Then, model performance was assessed by the remaining 30%. In model selection, we evaluated different combinations of connection, the number of hidden layers, and the number of neurons in each hidden layer.
Results
Based on results, biorhythm cycles (physical biorhythm and emotional biorhythm) and duration of symptoms had second and third importance after demographic factors (age, hypertension, addiction, and Q2 therapy) in predicting the duration of hospitalization. And the two variables of age (22.3%) and addiction (16.6%) contribute the most to importance in the demographic section.
Conclusion
So the number of hospitalization days decreased when the disease was controlled by providing adequate training for affected individuals, conducting regular and periodic screenings for early diagnosis of mental illnesses, consuming a high-fiber, low-carb, low-fat diet, receiving proper sleep training, utilizing cognitive behavioral therapies, participating in resistance sports. Additionally, replacing long-term care centers such as nursing homes where educated nurses provide services to patients, will impose less cost on the government as compared to hospitals.
Comparison of bleb morphologies between phacoemulsification combined with Ex-PRESS minishunt implantation, phacotrabeculectomy and trabeculectomy alone: a two-year retrospective in vivo confocal microscopy study
Authors: Zhang Yuqiao , Lai Chunxin , Zhao Suwen , Li Ling , Luo Xiaoyang , Chen Yanlei , Niu Yongyi , Qin Yongjie , Zhang Hongyang 
Published: October 2023

Abstract
Background: To compare the bleb morphologies of phacoemulsification combined with Ex-PRESS implantation (Phaco-ExPRESS), phaco trabeculectomy (Phaco-Trab), and trabeculectomy (Trab) in postoperative two years.
Methods: Patients with primary open-angle glaucoma (POAG) with or without cataracts were included in this study. All patients underwent surgeries of either Phaco-ExPRESS, Phaco-Trab, or Trab. The morphologic structures of the filtering bleb, including microcysts area, hyperreflective dot density, and stromal connective tissue under in vivo confocal microscope (IVCM), were compared between the three groups. The data were collected preoperatively and postoperatively at 2 weeks, 1 month, 3 months, 6 months, 12 months, 18 months, and 24 months.
Results: Eighty-nine eyes from 89 patients were enrolled, including 32 in the Phaco-ExPRESS group, 25 in the Phaco-Trab group, and 32 in the Trab group. In a 24-month follow-up, bleb morphologies in Phaco-ExPRESS were similar to the Trab group. The area of epithelial microcysts was significantly increased in Phaco-ExPRESS and Trab groups while significantly decreased in Phaco-Trab. At postoperative 24 months, the complete success rate was 65.1% in Phaco-ExPRESS, 32.0% in Phaco-Trab, and 59.4% in the Trab group (P = 0.03). The phaco-Trab group had more postoperative anti-glaucoma medications than the other two groups (P < 0.05).
Conclusions: Phaco-ExPRESS group and Trab group had similar blebs morphologies in IVCM, with larger microcyst area, looser connective tissue, and less inflammation than Phaco-Trab, indicating that the function of blebs in the Phaco-ExPRESS and Trab group, was more potent than that of Phaco-Trab. All these surgical methods provided adequate IOP control, but Phaco-Trab required more anti-glaucoma medications.
A case vignette study to refine the target group of an intermediate care model: the Acute Geriatric Community Hospital
Authors: Kroeze Eline D. , de Groot Aafke J. , Smorenburg Susanne M. , Vroomen Janet L. MacNeil , Vught Anneke J.A.H. van , Buurman Bianca M. 
Published: October 2023

Abstract
Purpose
To refine the admission criteria of the Acute Geriatric Community Hospital (AGCH) by defining its target group boundaries with (geriatric) hospital care and other bed-based intermediate care models.
Methods
A qualitative study consisting of a three-phase refinement procedure with case vignettes. Physicians, medical specialists, nurse practitioners, and physician assistants in hospitals (n = 10) or intermediate care facilities (n = 10) in the Netherlands participated. They collected case vignettes from clinical practice (phase one). The referral considerations and decisions for each case were then documented through surveys (phase two) and two focus groups (phase 3). For thematic data analysis, inductive and deductive approaches were used.
Results
The combination of medical specialist care (MSC) and medical generalist care (MSG), is unique for the AGCH compared to other intermediate care models. Compared to (geriatric) hospital care, the AGCH offers a more limited scope of MSC. Based on these findings, 13 refined admission criteria were developed such as ‘The required diagnostic tests to monitor the effectiveness of treatment are available at the AGCH’. Besides admission criteria, additional clinical and organizational considerations played a role in referral decision-making; 10 themes were identified.
Conclusion
This case vignette study defined the target group boundaries between the AGCH and other care models, allowing us to refine the AGCH admission criteria. Our findings may help to determine the required competencies of the interdisciplinary AGCH team and to develop triage instruments. The identified consideration themes can be used as conceptual framework in further research.
Determinants of Virologic Failure Among Adult Hiv Patients on First Line Atiretroviral Treatment at Oromia, Central Ethiopia: 2022 a Case –control Study
Authors: Ayana Worku Gidisa , Ayana Mulatu , Dechasa Abebe 
Published: October 2023

Abstract
Background: The problem of viral failure among first line antiretroviral treatment is growing in Ethiopia despite the 3rd 95’s target of 2025.Ethiopia's viral suppression rate was less than 90% by 2020 and more than 10% of adult client on ART in Woliso Town were unsuppressed at the end of March 2022. This study aims to identify determinants of virologic failure among adult clients on first line ART at Health facilities in Oromia region, Ethiopia
Methods: Facility based unmatched case-control study was conducted at health facilities at Oromia region, Ethiopia from August 1 to September 01, 2022. A total of 135 cases and 268 controls participants were selected using simple random sampling technique and data were collected by review of client’s document. Epi-info7was used for data entry and SPSS version 20 for data analysis. Variables having a P-value of less than 0.25 in bi-variable analysis were included in multivariable logistic regression. Determinants of virologic failure were determined based on adjusted odds ratio using 95 % CI and at a P-value of <0.05.
Result: In this study, clients with age ≥ 35 years (AOR =3.4, 95% CI: 1.6, 7.0), clients with a baseline regimen of AZT+3TC+NVP (AOR=3.5, 95% CI:1.4,8.8), clients with base-line CD4 count <350 mm3 (AOR=2.3,95%CI:1.1,4.5),clients who had history of appointment spacing model (AOR=0.05,95%CI:0.03,0.10), being single marital status(AOR=3.7,95% CI:1.4,10.5),TB-HIV coinfection(AOR=2.58, 95% CI:1.3,5.1) and having opportunistic infection in last six months (AOR=3.06,95%CI: 1.5,6.3) were factors significantly associated with virologic failure.
Conclusion: This study showed that age ≥ 35 years, being single, baseline ART regimen with (AZT+3TC+NVP), Baseline CD4 cell count <350 mm3, Tb-co infection, opportunistic infection in the last 6 months were factors associated with virologic failure. Involvement to appointment spacing model was found to be protective for.