bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laser-assisted direct roller imprinting of large-area microstructured optical surfaces
Authors: Nagato Keisuke , Takahashi Ken , Yajima Yuki , Nakao Masayuki 
Published: January 2024

AbstractIn this study, a high-throughput fabrication method called laser-assisted direct roller imprinting (LADRI) was developed to lower the cost of nanoimprinting large-area polymer films and to address problems associated with nanoimprinting, namely, microstructural damage and precision in flatness of entire film. With LADRI, the laser directly heats the microstructured surface of the roller mold, which heats and melts the surface of a polymethyl methacrylate (PMMA) film to replicate the microstructures on the mold rapidly. In this study, the effects of laser power density, scanning speed, size of the microstructures, and contact pressure on the replication speed were investigated experimentally. The replication speed increased as the power and scanning speed increased. However, because the film required heating until it filled the entire depth of the microstructure, an appropriate replication speed was necessary. This result was supported by simulation of the temperature distribution inside the mold and the PMMA using transient heat conduction analyses. To demonstrate the applications of LADRI, two different optical surfaces were replicated: an antireflection (AR) structure with conical structures sized several hundred nanometers and a light-extraction structure with a microlens array (MLA) comprising 10 μm lenses, for display and illumination, respectively. The replication degree of the MLA was governed by the contact pressure. Polymer flow simulation indicated that the heat conduction and flow speeds of the melted PMMA surface were comparable within several tens of micrometers. In addition, the reflectivity of the AR structure decreased from 4 to 0.5%, and the light intensity of the light-extraction structure increased by a factor of 1.47.
Gatad2b, associated with the neurodevelopmental syndrome GAND, plays a critical role in neurodevelopment and cortical patterning
Authors: Abad Clemer , Robayo Maria C. , Muñiz-Moreno Maria del Mar , Bernardi Maria T. , Otero Maria G. , Kosanovic Christina , Griswold Anthony J. , Pierson Tyler Mark , Walz Katherina , Young Juan I. 
Published: January 2024

AbstractGATAD2B (GATA zinc finger domain containing 2B) variants are associated with the neurodevelopmental syndrome GAND, characterized by intellectual disability (ID), infantile hypotonia, apraxia of speech, epilepsy, macrocephaly and distinct facial features. GATAD2B encodes for a subunit of the Nucleosome Remodeling and Histone Deacetylase (NuRD) complex. NuRD controls transcriptional programs critical for proper neurodevelopment by coupling histone deacetylase with ATP-dependent chromatin remodeling activity. To study mechanisms of pathogenesis for GAND, we characterized a mouse model harboring an inactivating mutation in Gatad2b. Homozygous Gatad2b mutants die perinatally, while haploinsufficient Gatad2b mice exhibit behavioral abnormalities resembling the clinical features of GAND patients. We also observed abnormal cortical patterning, and cellular proportions and cell-specific alterations in the developmental transcriptome in these mice. scRNAseq of embryonic cortex indicated misexpression of genes key for corticogenesis and associated with neurodevelopmental syndromes such as Bcl11b, Nfia and H3f3b and Sox5. These data suggest a crucial role for Gatad2b in brain development.
Redox signalling regulates breast cancer metastasis via phenotypic and metabolic reprogramming due to p63 activation by HIF1α
Authors: Ren Zuen , Dharmaratne Malindrie , Liang Huizhi , Benard Outhiriaradjou , Morales-Gallego Miriam , Suyama Kimita , Kumar Viney , Fard Atefeh Taherian , Kulkarni Ameya S. , Prystowsky Michael , Mar Jessica C. , Norton Larry , Hazan Rachel B. 
Published: January 2024

Abstract
Background
Redox signaling caused by knockdown (KD) of Glutathione Peroxidase 2 (GPx2) in the PyMT mammary tumour model promotes metastasis via phenotypic and metabolic reprogramming. However, the tumour cell subpopulations and transcriptional regulators governing these processes remained unknown.

Methods
We used single-cell transcriptomics to decipher the tumour cell subpopulations stimulated by GPx2 KD in the PyMT mammary tumour and paired pulmonary metastases. We analyzed the EMT spectrum across the various tumour cell clusters using pseudotime trajectory analysis and elucidated the transcriptional and metabolic regulation of the hybrid EMT state.

Results
Integration of single-cell transcriptomics between the PyMT/GPx2 KD primary tumour and paired lung metastases unraveled a basal/mesenchymal-like cluster and several luminal-like clusters spanning an EMT spectrum. Interestingly, the luminal clusters at the primary tumour gained mesenchymal gene expression, resulting in epithelial/mesenchymal subpopulations fueled by oxidative phosphorylation (OXPHOS) and glycolysis. By contrast, at distant metastasis, the basal/mesenchymal-like cluster gained luminal and mesenchymal gene expression, resulting in a hybrid subpopulation using OXPHOS, supporting adaptive plasticity. Furthermore, p63 was dramatically upregulated in all hybrid clusters, implying a role in regulating partial EMT and MET at primary and distant sites, respectively. Importantly, these effects were reversed by HIF1α loss or GPx2 gain of function, resulting in metastasis suppression.

Conclusions
Collectively, these results underscored a dramatic effect of redox signaling on p63 activation by HIF1α, underlying phenotypic and metabolic plasticity leading to mammary tumour metastasis.
The multifaceted role of Fragile X-Related Protein 1 (FXR1) in cellular processes: an updated review on cancer and clinical applications
Authors: Khan Faiz Ali , Fang Na , Zhang Weijuan , Ji Shaoping 
Published: January 2024

AbstractRNA-binding proteins (RBPs) modulate the expression level of several target RNAs (such as mRNAs) post-transcriptionally through interactions with unique binding sites in the 3′-untranslated region. There is mounting information that suggests RBP dysregulation plays a significant role in carcinogenesis. However, the function of FMR1 autosomal homolog 1(FXR1) in malignancies is just beginning to be unveiled. Due to the diversity of their RNA-binding domains and functional adaptability, FXR1 can regulate diverse transcript processing. Changes in FXR1 interaction with RNA networks have been linked to the emergence of cancer, although the theoretical framework defining these alterations in interaction is insufficient. Alteration in FXR1 expression or localization has been linked to the mRNAs of cancer suppressor genes, cancer-causing genes, and genes involved in genomic expression stability. In particular, FXR1-mediated gene regulation involves in several cellular phenomena related to cancer growth, metastasis, epithelial-mesenchymal transition, senescence, apoptosis, and angiogenesis. FXR1 dysregulation has been implicated in diverse cancer types, suggesting its diagnostic and therapeutic potential. However, the molecular mechanisms and biological effects of FXR1 regulation in cancer have yet to be understood. This review highlights the current knowledge of FXR1 expression and function in various cancer situations, emphasizing its functional variety and complexity. We further address the challenges and opportunities of targeting FXR1 for cancer diagnosis and treatment and propose future directions for FXR1 research in oncology. This work intends to provide an in-depth review of FXR1 as an emerging oncotarget with multiple roles and implications in cancer biology and therapy.
Glucocorticoid activates STAT3 and NF-κB synergistically with inflammatory cytokines to enhance the anti-inflammatory factor TSG6 expression in mesenchymal stem/stromal cells
Authors: Huang Peiqing , Sun Rongrong , Xu Chenchang , Jiang Zixuan , Zuo Muqiu , Li Yinghong , Liu Rui , Gong Pixia , Han Yuyi , Fang Jiankai , Li Peishan , Shao Changshun , Shi Yufang 
Published: January 2024

AbstractGlucocorticoid (GC) is essential for maintaining immune homeostasis. While GC is known to regulate the expression of genes related to inflammation in immune cells, the effects of GC, especially in the presence of inflammation, on non-immune cells remain largely unexplored. In particular, the impact of GC on inflammatory cytokine-induced immune modulatory responses of tissue stromal cells is unknown, though it has been widely used to modulate tissue injuries. Here we found that GC could enhance the expression of TSG6, a vital tissue repair effector molecule, in IFNγ and TNFα treated human umbilical cord (UC)-MSCs. NF-κB activation was found to be required for GC-augmented TSG6 upregulation. STAT3, but not STAT1, was also found to be required for the TSG6 upregulation in MSCs exposed to IFNγ, TNFα and GC. Moreover, the phosphorylation (activation) of STAT3 was attenuated when NF-κB was knocked down. Importantly, human UC-MSCs pretreated with a cocktail containing GC, IFNγ, and TNFα could significantly enhance the therapeutic effect of human UC-MSCs in an acute lung injury mouse model, as reflected by reduced infiltration of immune cells and down-regulation of iNOS in macrophages in the lung. Together, the findings reveal a novel link between GR, NF-κB and STAT3 in regulating the immunomodulatory and regenerative properties of MSCs, providing novel information for the understanding and treatment of inflammatory conditions.
Glucocorticoid stress hormones stimulate vesicle-free Tau secretion and spreading in the brain
Authors: Yu Qing , Du Fang , Belli Irla , Gomes Patricia A. , Sotiropoulos Ioannis , Waites Clarissa L. 
Published: January 2024

AbstractChronic stress and elevated levels of glucocorticoids (GCs), the main stress hormones, accelerate Alzheimer’s disease (AD) onset and progression. A major driver of AD progression is the spreading of pathogenic Tau protein between brain regions, precipitated by neuronal Tau secretion. While stress and high GC levels are known to induce intraneuronal Tau pathology (i.e. hyperphosphorylation, oligomerization) in animal models, their role in trans-neuronal Tau spreading is unexplored. Here, we find that GCs promote secretion of full-length, primarily vesicle-free, phosphorylated Tau from murine hippocampal neurons and ex vivo brain slices. This process requires neuronal activity and the kinase GSK3β. GCs also dramatically enhance trans-neuronal Tau spreading in vivo, and this effect is blocked by an inhibitor of Tau oligomerization and type 1 unconventional protein secretion. These findings uncover a potential mechanism by which stress/GCs stimulate Tau propagation in AD.
The association between blood pressure control in women during pregnancy and adverse perinatal outcomes: the TMM BirThree Cohort Study
Authors: Ishikuro Mami , Obara Taku , Murakami Keiko , Ueno Fumihiko , Noda Aoi , Onuma Tomomi , Kikuya Masahiro , Metoki Hirohito , Kuriyama Shinichi 
Published: January 2024

AbstractBlood pressure (BP) control in pregnancy is essential to prevent adverse outcomes. However, BP levels for hypertension treatment are inconsistent among various guidelines. This study investigated the association between BP control and adverse perinatal outcomes. A total of 18,155 mother-offspring pairs were classified into four groups according to BP after 20 gestational weeks: normal BP (<140/90 mmHg without antihypertensive drugs), high BP (≥140/90 mmHg without antihypertensive drugs), controlled BP (<140/90 mmHg with antihypertensive drugs), and uncontrolled BP (≥140/90 mmHg with antihypertensive drugs). The prevalence of small for gestational age was 1,087/17,476 offspring in normal BP, 78/604 in high BP, 5/42 in controlled BP, and 7/33 in uncontrolled BP. Compared to normal BP, adjusted odds ratios (ORs) (95% confidence intervals (CIs)) were 1.76 (1.32–2.35) for high BP, 2.08 (0.79–5.50) for controlled BP, and 2.34 (0.94–5.85) for uncontrolled BP (multiple logistic regression analysis). Similarly, the adjusted ORs (95% CIs) were 1.80 (1.35–2.41), 3.42 (1.35–8.63), and 5.10 (1.93–13.45) for high, controlled, and uncontrolled BPs for low birth weight, respectively; 1.99 (1.48–2.68), 2.70 (1.12–6.50), and 6.53 (3.09–13.82) for high, controlled, and uncontrolled BPs for preterm birth, respectively; 1.64 (1.19–2.24), 2.17 (0.88–5.38), and 2.12 (0.80–5.65) for high, controlled, and uncontrolled BPs for admission to the Neonatal Intensive Care Unit or Growing Care Unit, respectively; and 1.17 (0.70–1.95), 2.23 (0.65–7.68), and 0.91 (0.20–4.16) for high, controlled, and uncontrolled BPs for 1-min Apgar score < 7, respectively. BP ≥ 140/90 mmHg might be taken care for preventing various adverse perinatal outcomes.
Giant optical polarisation rotations induced by a single quantum dot spin
Authors: Mehdi E. , Gundín M. , Millet C. , Somaschi N. , Lemaître A. , Sagnes I. , Le Gratiet L. , Fioretto D. A. , Belabas N. , Krebs O. , Senellart P. , Lanco L. 
Published: January 2024

AbstractIn the framework of optical quantum computing and communications, a major objective consists in building receiving nodes implementing conditional operations on incoming photons, using a single stationary qubit. In particular, the quest for scalable nodes motivated the development of cavity-enhanced spin-photon interfaces with solid-state emitters. An important challenge remains, however, to produce a stable, controllable, spin-dependent photon state, in a deterministic way. Here we use an electrically-contacted pillar-based cavity, embedding a single InGaAs quantum dot, to demonstrate giant polarisation rotations induced on reflected photons by a single electron spin. A complete tomography approach is introduced to extrapolate the output polarisation Stokes vector, conditioned by a specific spin state, in presence of spin and charge fluctuations. We experimentally approach polarisation states conditionally rotated by $$\frac{\pi }{2}$$

π

2

, π, and $$-\frac{\pi }{2}$$
−

π

2

in the Poincaré sphere with extrapolated fidelities of (97 ± 1) %, (84 ± 7) %, and (90 ± 8) %, respectively. We find that an enhanced light-matter coupling, together with limited cavity birefringence and reduced spectral fluctuations, allow targeting most conditional rotations in the Poincaré sphere, with a control both in longitude and latitude. Such polarisation control may prove crucial to adapt spin-photon interfaces to various configurations and protocols for quantum information.
Skeletal editing of pyridines through atom-pair swap from CN to CC
Authors: Cheng Qiang , Bhattacharya Debkanta , Haring Malte , Cao Hui , Mück-Lichtenfeld Christian , Studer Armido 
Published: January 2024

AbstractSkeletal editing is a straightforward synthetic strategy for precise substitution or rearrangement of atoms in core ring structures of complex molecules; it enables quick diversification of compounds that is not possible by applying peripheral editing strategies. Previously reported skeletal editing of common arenes mainly relies on carbene- or nitrene-type insertion reactions or rearrangements. Although powerful, efficient and applicable to late-stage heteroarene core structure modification, these strategies cannot be used for skeletal editing of pyridines. Here we report the direct skeletal editing of pyridines through atom-pair swap from CN to CC to generate benzenes and naphthalenes in a modular fashion. Specifically, we use sequential dearomatization, cycloaddition and rearomatizing retrocycloaddition reactions in a one-pot sequence to transform the parent pyridines into benzenes and naphthalenes bearing diversified substituents at specific sites, as defined by the cycloaddition reaction components. Applications to late-stage skeletal diversification of pyridine cores in several drugs are demonstrated.
Emergent seesaw oscillations during cellular directional decision-making
Authors: Ron Jonathan E. , Crestani Michele , Kux Johan M. , Liu Jiayi , Al-Dam Nabil , Monzo Pascale , Gauthier Nils C. , Sáez Pablo J. , Gov Nir S. 
Published: January 2024

AbstractMotile cells inside living tissues often encounter junctions, where their path branches into several alternative directions of migration. We present a theoretical model of cellular polarization for a cell migrating along a one-dimensional line, arriving at a symmetric Y junction and extending protrusions along the different paths that originate at the junction. The model predicts the spontaneous emergence of deterministic oscillations of growth and cellular polarization between competing protrusions during the directional decision-making process. The oscillations are modified by cellular noise but remain a dominant feature that affects the time it takes the cell to migrate across the junction. These predictions are confirmed experimentally for two different cell types (non-cancerous endothelial and cancerous glioma cells) migrating on a patterned network of thin adhesive lanes with junctions.
Square beams for optimal tiling in transmission electron microscopy
Authors: Chua Eugene Y. D. , Alink Lambertus M. , Kopylov Mykhailo , Johnston Jake D. , Eisenstein Fabian , de Marco Alex 
Published: January 2024

AbstractImaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem for dose-sensitive samples. Here, we introduce a square electron beam that can easily be retrofitted in existing microscopes, and demonstrate its application, showing that it can tile nearly perfectly and deliver cryo-electron microscopy imaging with a resolution comparable to conventional set-ups.
A comparative wordlist for investigating distant relations among languages in Lowland South America
Authors: Blum Frederic , Barrientos Carlos , Zariquiey Roberto , List Johann-Mattis 
Published: January 2024

AbstractThe history of the language families in Lowland South America remains an understudied area of historical linguistics. Panoan and Tacanan, two language families from this area, have frequently been proposed to descend from the same ancestor. Despite ample evidence in favor of this hypothesis, not all scholars accept it as proven beyond doubt. We compiled a new lexical questionnaire with 501 basic concepts to investigate the genetic relation between Panoan and Tacanan languages. The dataset includes data from twelve Panoan, five Tacanan, and four other languages which have previously been suggested to be related to Pano-Tacanan. Through the transparent annotation of grammatical morphemes and partial cognates, our dataset provides the basis for testing language relationships both qualitatively and quantitatively. The data is not only relevant for the investigation of the ancestry of Panoan and Tacanan languages. Reflecting the state of the art in computer-assisted approaches for historical language comparison, it can serve as a role model for linguistic studies in other areas of the world.
Influence of acculturation and cultural values on the self-reference effect
Authors: Gilliam Ashley N. , Gutchess Angela 
Published: January 2024

AbstractCultural milieu can influence the way information is processed and what strategies are employed to deal with ever-changing environments. This study assessed whether acculturation and cultural values of East Asians can affect memory, with a specific focus on the self-reference effect in Chinese international students. Participants encoded and retrieved adjectives, with some trials relating the words to the self (i.e., the self-referencing task), another person, or a control condition; participants also completed questionnaires assessing cultural adaptation and self-construal. Results did not show a relationship between acculturation orientation and self-construal and the magnitude of the self-reference effect in memory, defined as better memory for adjectives encoded related to the self compared to those related to close others, in this sample of Chinese international students. Future research should explore effects of acculturation over time, incorporating more heterogeneous samples and sensitive neural measures.
Association of frailty with mortality in cancer survivors: results from NHANES 1999–2018
Authors: Zhai Chongya , Yin Luxi , Shen Jiaying , Dong Jie , Zheng Yu , Pan Hongming , Han Weidong 
Published: January 2024

AbstractCancer survivors are vulnerable to frailty. While few studies have focused on the association of frailty with mortality risk among cancer survivors, the current study aimed to reveal this association. In this cohort study, 4723 cancer survivors were enrolled from the National Health and Nutrition Examination Surveys (NHANES, 1999–2018). Frailty status was quantified using the 53-item frailty index. Death outcomes were linked to National Death Index mortality data (as of December 31, 2019). Cox proportional hazard models were used to estimate HRs (95% CIs). The median (IQR) frailty score was 0.190 (0.132, 0.277). During the median follow-up of 6.7 years, 1775 all-cause deaths (including 581 cancer deaths and 385 cardiac deaths) were documented. Compared to the lowest tertile of frailty scores, the adjusted HRs (95% CIs) for the highest tertile were 2.698 (2.224, 3.272) for all-cause mortality (P trend < 0.001), 2.145 (1.547, 2.973) for cancer mortality (P trend < 0.001), and 3.735 (2.231, 6.251) for cardiac mortality (P trend < 0.001). Moreover, a positive dose‒response association between the frailty score and mortality risk was determined. Each per-unit increase in the frailty score (natural logarithm transformed) was found to increase all-cause mortality by 159% (P < 0.001), cancer mortality by 103% (P < 0.001), and cardiac mortality by 256% (P < 0.001). A consistent result was shown when stratifying by age, sex, race, body mass index, and type of cancer. This study suggested that the frailty index was positively associated with all-cause mortality and cause-specific mortality (including cancer and cardiac deaths) among cancer survivors.
Correlation of CT-based bone mineralization with drilling-force measurements in anatomical specimens is suitable to investigate planning of trans-pedicular spine interventions
Authors: Wolff Stefanie , Adler Simon , Eppler Elisabeth , Fischer Karin , Lux Anke , Rothkötter Hermann-Josef , Skalej Martin 
Published: January 2024

AbstractThis interdisciplinary study examined the relationship between bone density and drilling forces required during trans-pedicular access to the vertebra using fresh–frozen thoraco-lumbar vertebrae from two female body donors (A, B). Before and after biomechanical examination, samples underwent high-resolution CT-quantification of total bone density followed by software-based evaluation and processing. CT density measurements (n = 4818) were calculated as gray values (GV), which were highest in T12 for both subjects (GVmaxA = 3483.24, GVmaxB = 3160.33). Trans-pedicular drilling forces F (Newton N) were highest in L3 (FmaxB = 5.67 N) and L4 (FmaxA = 5.65 N). In 12 out of 13 specimens, GVs significantly (p < 0.001) correlated with force measurements. Among these, Spearman correlations r were poor in two lumbar vertebrae, fair in five specimens, and moderately strong in another five specimens, and highest for T11 (rA = 0.721) and L5 (rB = 0.690). Our results indicate that CT-based analysis of vertebral bone density acquired in anatomical specimens is a promising approach to predict the drilling force appearance as surrogate parameter of its biomechanical properties by e.g., linear regression analysis. The study may be of value as basis for biomechanical investigations to improve planning of the optimal trajectory and to define safety margins for drilling forces during robotic-assisted trans-pedicular interventions on the spine in the future.