bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hematology and plasma chemistry values in free‐ranging Humboldt penguins (Spheniscus humboldti) in Chile
Authors: Wallace Roberta S. , Teare J. Andrew , Diebold Edward , Michaels Margaret , Willis Mary Jo 
Published: June 2005

AbstractTo establish baseline hematologic and plasma biochemistry values in free‐ranging Humboldt penguins (Spheniscus humboldti), heparinized blood samples were collected from 51 apparently healthy, adult Humboldt penguins residing at two colonies off the Chilean coast. Thirty samples were collected in April, 1992, from penguins inhabiting the Ex‐islote de los Pájaros Niños in Algarrobo, Chile. In September, 1992, 21 samples were collected from birds inhabiting Isla de Cachagua, Chile. Hematologic values measured include packed cell volume, leucocyte count, leucocyte differential, and the presence of blood parasites. Plasma biochemistry values measured include glucose, blood urea nitrogen, creatinine, uric acid, calcium, inorganic phosphorous, sodium, potassium, chloride, total protein, albumin, globulin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, and creatine kinase. Only the mean values for chloride and for the number of eosinophils differed significantly between the two sample groups. No blood parasites were seen. © 1995 Wiley‐Liss, Inc.
Enclosure utilization and activity of Round Island geckos (Phelsuma guentheri)
Authors: Wheler Colette L. , Fa John E. 
Published: June 2005

AbstractThis study evaluated space use and activity of individually housed Phelsuma guentheri to formulate recommendations for environmental enrichment. Gecko location within small (0.03 m3) and large (0.22 m3) vivaria and substrate use data were collected over five 24 h periods. Thermogradients within each enclosure type were determined by measuring temperature at various heights. From these data, geckos' space use, activity cycle, and substrate preference were calculated in relation to temperature changes and photoperiod.Geckos did not utilize their environment uniformly. Heavily utilized locations contained cage furnishings or hides. Geckos avoided the use of vertical glass walls. Thermogradients were not actively utilized; however, when given the opportunity, geckos would orient towards natural sunlight. Enclosure size did not affect activity cycle, which was nocturnal with crepuscular peaks.Recommendations for enclosure improvement include provision of more furnishings and hides at different thermal levels. An alternate focal heat source other than UV light is recommended to provide warmth without light. Larger enclosures may encourage more uniform use of available space. Transparent glass may not be an appropriate substrate for these geckos.Once recommendations have been implemented, a similar study must be conducted to assess benefit. © 1995 Wiley‐Liss, Inc.
Immunocontraception of captive exotic species. I. Przewalski's horses (Equus przewalskii) and banteng (Bos javanicus)
Authors: Kirkpatrick Jay F. , Zimmermann Waltraut , Kolter Lydia , Liu I. K. M. , Turner J. W. 
Published: June 2005

AbstractA contraceptive vaccine made of porcine zonae pellucidae (PZP) was tested in three Przewalski's mares and five banteng cows. The vaccine antigen consisted of the complete family of glycoproteins of the porcine zona pellucida, including the sperm receptor ZP3. All mares and three of five banteng were inoculated with 2 or 3 i.m. injections of approximately 65 μg of antigen given over a 6 week period. Two other banteng received inoculations of only 35 μg of antigen on the same schedule. Two of the three mares and three of five banteng cows were pregnant at the time of inoculation. No new pregnancies, as a result of postinoculation breedings, occurred among either the mares 36 months after 65 μg antigen inoculations or among the banteng for 24 months after 65 μg inoculations. One postinoculation pregnancy resulted among the two banteng receiving only 35 μg of antigen. Differences in fertility between treated and control mares and between preinoculation and postinoculation reproductive performance of the banteng were significant (P < 0.05). Urinary ovarian steroid metabolites and behavioral observations indicated follicular development and ovulations were occurring among treated mares during the year following PZP inoculations. PZP immunization produced progressively elevated anti‐PZP antibodies in both species, which provided contraceptive protection. PZP immunization appears to be an effective form of contraception in both species. © 1995 Wiley‐Liss, Inc.
Interaction sequences between chimpanzees and human visitors at the Zoo
Authors: Cook S. , Hosey G. R. 
Published: June 2005

AbstractData were collected on the behavior and physical characteristics of 259 human visitors and 24 chimpanzees at Chester Zoo. The successive responses of humans and chimpanzees to each other's behavior were recorded, the resulting long sequence being referred to as an interaction sequence. There was no particular set of characteristics that distinguished interactors from noninteractors in either humans or chimpanzees, although there was some evidence that chimpanzees were particularly likely to respond to men carrying objects. Chimpanzee responses were random with respect to the previous human behavior, but human responses were significantly associated with the preceding chimpanzee behavior. In particular, chimpanzee sounds were likely to be followed by human sounds, and begging was likely to be followed by the offer of food. Interaction sequences varied in length, but 9% of chimpanzee‐initiated sequences went as far as a ninth interaction. Sequences resulted in the chimpanzees being given food in 25% of human‐initiated, but only 8% of chimpanzee‐initiated sequences. The results are consistent with the interpretation that humans and chimpanzees are motivated to interact with one another and that the chimpanzees do this primarily to obtain food. © 1995 Wiley‐Liss, Inc.
Protein requirements in tropical countries: nitrogen losses in sweat and their relation to nitrogen balance
Authors: Ashworth Ann , Harrower A. D. B. 
Published: June 2005

1. An experiment was undertaken to determine whether high rates of sweating in a tropical climate affect protein requirements by increasing the total nitrogen losses from the body.2. Six fully acclimatized volunteers were given a diet supplying 50 g protein (= 8 g N) daily. They performed strenuous physical work of a normal nature for an average of 6½ h a day for two 5-day periods. During control periods the subjects took minimal exercise and lived in a cool environment. N balance was measured throughout.3. Rates of sweating were measured by weighing. Whole body sweat was collected and the concentrations were measured of nitrogen, sodium and potassium. During 6½ h work approximately 3 l. of sweat were lost, containing on average 0·49 g N, 64 m-equiv. Na and 22 m-equiv. K.4. The N concentration in sweat was 0·20 mg/g, which is lower than that found by most other workers. It is suggested that acclimatization is an important factor in reducing N loss by sweating.5. There was a marked decrease in urinary Na excretion during sweating, which compensated fully for the loss of Na in sweat. Renal compensation for loss of K was less efficient.6. Because the total N loss in sweat was small, it was not possible to establish with certainty whether it was compensated for by a reduced renal excretion of N. However, after the initial period the subjects were in N balance in spite of the relatively low protein intake.7. It is concluded that there is no evidence to suggest that heavy sweating under natural conditions in a tropical climate causes a significant increase in protein requirements.
Effect of groundnut meal containing aflatoxin on Cynomolgus monkeys
Authors: Cuthbertson W. F. J. , Laursen A. C. , Pratt D. A. H. 
Published: June 2005

1. Both male and female Cynomolgus (Macaca irus) monkeys survived for 3 years without apparent ill health when fed on diets containing groundnut meal to provide up to 0·36 μg aflatoxin B1per g diet and thus supplying a mean daily consumption of up to 2 μg aflatoxin B1per kg body-weight. No histological changes attributable to aflatoxicosis were discovered in any of the organs from the monkeys receiving these quantities of aflatoxin.2. Few monkeys survived for more than a month or two when given a diet containing 1·8 μg aflatoxin B1per g, which provided about 50 μg aflatoxin B1per kg body-weight per day.3. No depression in growth rate nor effect on health was noted in those monkeys which survived on diets containing 1·8 μg aflatoxin B1, nor in any of the other monkeys.4. Histological changes were observed in the livers of all monkeys receiving the diet containing 1·8 μg aflatoxin B1per g for more than a month or two, but no abnormalities related to aflatoxicosis could be detected in any of the other organs, except for minor changes in the kidneys of two monkeys.5. No tumours were seen in any of the monkeys, even in those surviving for 3 years on diets providing 1·8 μg aflatoxin B1/g. Thus, no conclusions can be drawn from this work as to the carcinogenicity of aflatoxin in monkeys (still less in man), because the animals were young and little is known of the duration of exposure required to demonstrate carcinogenicity in this species.
The nutritional value of poor proteins fed at high levels
Authors: Carpenter K. J. , Anantharaman K. 
Published: June 2005

1. Groundnut flour plus lysine, fed to young rats at a high level as their sole source of protein supported weight gain and nitrogen retention equal to that on what has been considered the ‘ideal’ egg protein diet. For every 100 metabolizable kcal of the groundnut diet that were consumed, 41 came from the dietary protein and there was a net retention of protein equivalent to 14·5 kcal.2. The predicted retention of protein according to the equations of Miller & Payne (1961) for this methionine-deficient protein source, given a score of 56 by FAO (1957), would have been equivalent to only 6·1 kcal and, whatever the level of such a protein in a diet, the maximum predicted retention would be equivalent to no more than 8·5 kcal, or 60% of the best expected with egg as the protein source.3. Feeding a lysine-deficient protein source, wheat gluten, at high levels also gave a greater N retention than had previously been predicted.4. It is concluded that the Miller & Payne (1961, 1963) equations can greatly under-estimate the performance to be obtained from feeding poor proteins at high levels. A revised equation (P. R. Pane, Private communication) predicts much higher results under these conditions.
Digestion of concentrate and of hay diets in the stomach and intestines of ruminants
Authors: Topps J. H. , Kay R. N. B. , Goodall E. D. 
Published: June 2005

1. The flow of digesta to the abomasum and through the duodenum and terminal ileum was measured over 24 h periods in sheep. Pelleted diets of concentrates, principally composed of barley, and of poor-quality hay were given. The seven Scottish Blackface ewes studied were all fitted with rumen cannulas, and in addition two had simple abomasal cannulas, one a re-entrant abomasal cannula, two re-entrant duodenal cannulas, and two re-entrant ileal cannulas.2. Paper impregnated with chromium sesquioxide was given twice daily by rumen fistula. The amounts of dry matter, starch, cellulose, total nitrogen and energy passing through the abomasum, duodenum and ileum and the amounts excreted in the faeces were measured. The flows of digesta were adjusted to give 100% recovery of chromium sesquioxide and the extent of digestion in various parts of the alimentary tract was calculated using these adjusted values. Concentrations of glucose in the blood and of volatile fatty acids (VFA) in the rumen were also measured.3. For the concentrate diet, 69% of the digestible dry matter disappeared in the stomach (reticulo-rumen, omasum and abomasum), 17% in the smail intestine and 14% in the large intestine. Values for disappearance of digestible energy were 72% in the stomach, 23% in the small intestine and 5% in the large intestine. Of the 298 g starch fed daily only 6–35 g passed through the abomasum or duodenum and only 1–4 g reached the terminal ileum. The cellulose in the diet was poorly digested.4. For the hay diet, 67% of the digestible dry matter disappeared in the stomach, 22% in the small intestine and 11% in the large intestine. Values for disappearance of digestible energy were 81% in the stomach, 7% in the small intestine and 12% in the large intestine. Of the 33 g of starch consumed daily, from 5 to 13 g passed through the abomasum or duodenum. The cellulose in the hay was 59% digestible and virtually all this digestion took place in the stomach.5. The concentration of VFA and the proportion of propionate in the rumen fluid, 2·5 h after feeding, were considerably greater on the concentrate diet than on the hay diet, but diet had little influence on the concentration of blood glucose.6. The importance of the small amount of starch passing to the sheep's small intestine is discussed.
The fate of water in the rumen
Authors: Warner A. C. I. , Stacy B. D. 
Published: June 2005

1. A mathematical treatment is given of the marker technique for studying water balances in the rumen. The treatment is extended to cover non-steady-state conditions and emphasis is placed on the underlying assumptions and practical limitations of the technique.2. The 51Cr complex of ethylenediamine tetra-acetic acid (51Cr EDTA) was used as a marker in the rumen of sheep.3. The 51Cr EDTA entered 95–98% of the water in the rumen. Mixing in the rumen, though adequate, was usually not quite complete even 1·5 h after injection of the dose. The techniques of injecting the dose and of withdrawing the sample were found to be important; some techniques often led to poor mixing.4. Provided that the time of injection of the dose was suitably selected, reliable estimates of the volume of water in the rumen could readily be made, but reliable estimates of flow rates needed stringent attention to details of technique.
Further studies on the problem of goitre in Ceylon
Authors: Mahadeva K. , Seneviratne D. A. , Jayatilleke D. B. , Shanmuganathan S. Senthe , Premachandra P. , Nagarajah M. 
Published: June 2005

1. A comparative study of the iodine and calcium contents of diets in areas of Ceylon where goitre is endemic and non-endemic was carried out.2. The iodine content of food grown in non-endemic areas was, in most instances, considerably higher than in endemic areas.3. In endemic areas the daily average intake per person per day was well above the allowance of 100–150 μg recommended by most workers. The critical level of total daily iodine intake below which goitre becomes endemic in Ceylon was 400 μg. Possible reasons for this higher need of iodine are discussed.4. Addition of iodine to cooking salt has been used successfully to combat endemic goitre in many countries. The amount of iodine added is based on the degree of endemicity and the amount of salt consumed per person per day. It is suggested that the more important criteria which should determine the amount of iodine added to cooking salt are the dietary iodine content, the daily total intake of dietary iodine and particularly the level of iodine below which goitre becomes prevalent.
The effects of feeding toxic groundnut meal to growing pigs and its interaction with high-copper diets
Authors: Barber R. S. , Braude R. , Mitchell K. G. , Harding J. D. J. , Lewis G. , Loosmore R. M. 
Published: June 2005

1. Three experiments using sixty individually fed, enzootic pneumonia-free Large White pigs on experiment from 9–11 weeks of age to 200 lb live weight are described.2. Information was obtained on the effect of different dietary concentrations of aflatoxin B1 on the performance of the pigs, on various biochemical measurements and on the histology of the livers and kidneys.3. Reduced growth rate and loss of appetite were the main adverse effects observed, their extent being positively related to the dietary level of aflatoxin B1. No marked clinical signs were seen, mortality was very low and there was little or no effect on the feed conversion efficiency of the animals.4. No evidence for any adverse interaction between aflatoxin B1 and the presence in the diet of a supplement of copper sulphate providing 250 ppm Cu was apparent from either the performance, biochemical or histological results obtained. It was concluded that the few isolated reports of toxicity in growing pigs fed diets containing a supplement of 250 ppm Cu were unlikely to have been the result of the unsuspected presence of aflatoxin B1 in the feedingstuffs used in the diets.5. Aflatoxin B1 tended to increase the serum alkaline phosphatase level, the concentration of Cu in the kidneys, and liver weight and to reduce the liver vitamin A concentration. No other consistent differences in the measurements made were observed.6. The extent of the total pathological abnormality observed in the livers and kidneys was closely related to the level of aflatoxin B1 in the diet.
Metabolic responses to low doses of cyanocobalamin in patients with megaloblastic anaemia
Authors: Adams J. F. , Hume R. , Kennedy Elizabeth H. , Pirrie T. G. , Whitelaw J. W. , White A. M. 
Published: June 2005

1. The metabolic responses to daily treatment with 1 μg cyanocobalamin were studied in sixteen patients with megaloblastic anaemia due to cobalamin deficiency.2. The fall in serum iron was sluggish in most patients. The pattern of reticulocyte responses varied widely, the most common being a plateau with suboptimal maximal value.3. Urinary propionic acid excretion before treatment was normal in two out of six patients. A transient rise during treatment was observed in two patients, possibly owing to demands on coenzyme stores. The fall in urinary propionic acid excretion during treatment was slow.4. Serum vitamin B12levels rose during treatment. In two patients an abrupt rise was found, possibly owing to release of stored cobalamin into the circulation.
The effect of high salt intake or restricted water intake on diet selection by sheep
Authors: Wilson A. D. 
Published: June 2005

1. In three experiments sheep were offered a choice of two rations: one high in NaCl (7.5 or 15%) and one low in NaCl. The intake of each ration was recorded when fresh water was freely available, when increasing amounts of NaCl were added to the drinking water and when the amount of fresh water available was restricted.2. Low levels of NaCl in the drinking water or mild restriction of available water were without effect on the diet selected. At higher levels, total food intake was reduced and the reduction was sustained entirely by the high-salt ration. The intake of the low-salt ration remained the same or increased, so that the selected diet then contained an increased proportion of the low-salt ration. The effects of adding NaCl to the drinking water and of water restriction were similar.3. It was concluded that sheep with a choice of high- and low-salt rations can partly avoid the stress of saline drinking-water or restricted water supply by changing the proportion of each ration eaten.
Factors affecting the polyunsaturated fatty acid content of the plasma lipids of sheep
Authors: Moore J. H. , Noble R. C. , Steele W. 
Published: June 2005

1. In Expt 1, four adult wether sheep were given diets of hay or dried grass in an alternating sequence of feeding treatments. Each treatment period lasted for 20 days and blood samples were taken from the sheep on the last day of each period. When the diet of hay (linoleic: linolenic acid ratio 1.40) was replaced by one of dried grass (linoleic: linolenic acid ratio 0.28), there was an increase in the concentration of linolenic acid and a decrease in the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. There was an increase in the concentration of stearic acid and a decrease in the concentration of palmitic acid in the plasma triglycerides. Dietary change did not affect the composition of the plasma unesterified fatty acids.2. In Expt 2, two adult wether sheep, each with a rumen fistula, were given daily intraruminal infusions of 60 g of ‘linolenic’ or ‘linoleic’ acids (both about 70% pure) over a period of 5 days. The infusion treatments were then reversed. Blood samples were taken at the end of each infusion period. Intraruminal infusions of ‘linolenic’ acid increased the concentration of linolenic acid in the plasma cholesteryl esters and phospholipids and increased the concentration of stearic acid in the plasma triglycerides. The infusions of ‘linoleic’ acid increased the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. No appreciable changes were observed in the composition of the plasma unesterified fatty acids.
Efficiency of digestion in germ-free and conventional rabbits
Authors: Yoshida T. , Pleasants J. R. , Reddy B. S. , Wostmann B. S. 
Published: June 2005

1. Germ-free (GF) and conventional (CV) rabbits wearing collars to prevent coprophagy were fed an autoclaved diet with added cellulose. Their faecal excretion was analysed to determine nutrient digestibility.2. Clearly distinguishable hard faeces were excreted by the GF rabbit only if the diet contained at least 15% cellulose. Unlike CV rabbits, the GF rabbits did not consume their soft faeces even when permitted to do so. Soft faeces made up a larger proportion of the total output of GF than of CV rabbits. Food intake and total dry-matter excretion per kg body-weight were similar in both groups.3. Although digestibility of dry matter was similar in the two groups, in the GF rabbits there was a higher digestibility of crude fat and true protein and a lower digestibility of crude fibre and nitrogen-free extract. GF rabbits excreted a higher percentage of ingested calcium and phosphorus in the urine than did CV rabbits.4. The results suggest that intestinal microbes, even without the enhancing effect of coprophagy, aid in the digestion of carbohydrate by rabbits. The greater faecal excretion of crude fat and true protein by CV rabbits could result from poorer digestion and absorption, but could also represent nutrients synthesized by microbes from simpler materials. The reingestion of faecal crude fat and true protein might therefore improve the quality of the total nutrient intake. The results suggest ways of assuring an adequate dietary intake by GF rabbits in the absence of contributions from an intestinal microflora.