bluuebunny/crossref_metadata_2025_split huggingface dataset:
An altered plasma lipidome–phenome network characterizes heart failure with preserved ejection fraction
Authors: Jovanovic Nina , Foryst‐Ludwig Anna , Klose Christian , da Conceicao Cristina Rozados , Alasfar Lina , Birkner Till , Forslund Sofia K. , Kintscher Ulrich , Edelmann Frank 
Published: January 2024

AbstractAimsHeart failure with preserved ejection fraction (HFpEF) is a multifactorial, multisystemic syndrome that involves alterations in lipid metabolism. This study aimed to test whether distinct plasma lipid profiles or lipid entities or both are associated with clinical and functional echocardiographic parameters in HFpEF.Methods and resultsWe examined the human plasma lipidome in HFpEF patients (n = 18) with left ventricular ejection fraction ≥50% and N‐terminal pro‐brain natriuretic peptide (NT‐proBNP) >125 pg/mL and control subjects (n = 12) using mass spectrometry‐based shotgun lipidomics. The cohort included 8 women and 22 men with average age of 67.8 ± 8.6 SD. The control and disease groups were not significantly different with respect to age, body mass index, systolic and diastolic blood pressure, and waist‐to‐hip ratio. The disease group experienced more fatigue (P < 0.001), had more often coronary artery disease (P = 0.04), and received more medications (beta‐blockers, P < 0.001). The disease group had significantly different levels of HFpEF‐relevant parameters, including NT‐proBNP (P < 0.001), left ventricular mass index (P = 0.005), left atrial volume index (P = 0.001), and left ventricular filling index (P < 0.001), and lower left ventricular end‐diastolic diameter (P = 0.014), with no difference in left ventricular ejection fraction. Significant differences in lipid profiles between HFpEF patients and controls could not be detected, including no significant differences in abundance of circulating lipids binned by carbon chain length or by double bonds, nor at the level of individual lipid species. However, there was a striking correlation between selected lipids with smoking status that was independent of disease status, as well as between specific lipids and hyperlipidaemia [with corresponding significance of either false discovery rate (FDR) <0.1 or FDR < 0.01]. In an exploratory network analysis of correlations, we observed significantly stronger correlations within the HFpEF group between individual lipids from the cholesterol ester and phosphatidylcholine (PC) classes and clinical/echocardiographic parameters such as left atrial volume index, left ventricular end‐diastolic diameters, and heart rate (FDR < 0.1). In contrast, the control group showed significantly stronger negative correlations (FDR < 0.1) between individual species from the PC and sphingomyelin classes and left ventricular mass index or systolic blood pressure.ConclusionsWe did not find significant direct associations between plasma lipidomic parameters and HFpEF and therefore could not conclude that any specific lipids are biomarkers of HFpEF. The validation in larger cohort is needed to confidently conclude the absence of first‐order associations.
Cardiac magnetic resonance‐based layer‐specific strain in immune checkpoint inhibitor‐associated myocarditis
Authors: Li Zheng , Zhao Rui , Wang Cong , Wang Yan , Lin Jinyi , Zhao Shihai , Chen Jiahui , Zhou Yuhong , Liu Tianshu , Wang Feng , Shu Xianhong , Zeng Mengsu , Cheng Leilei 
Published: January 2024

AbstractAimsTo assess the different imaging characteristics between corticosteroid‐sensitive (CS) and corticosteroid‐refractory (CR) immune checkpoint inhibitor‐associated myocarditis (ICIaM) with cardiac magnetic resonance (CMR) and the potential CMR parameters in the early detection of CR ICIaM.Methods and resultsThirty‐five patients diagnosed with ICIaM and 30 age and gender‐matched cancer patients without a history of ICI treatment were enrolled. CMR with contrast was performed within 2 days of clinical suspicion. Left ventricular ejection fraction (LVEF) and late gadolinium enhancement (LGE) were assessed by CMR. LV sub‐endocardial (GLSendo) and sub‐epicardial (GLSepi) global longitudinal strains were quantified by offline feature tracking analysis. CS and CR ICIaM were defined based on the trend of Troponin I and clinical course during corticosteroid treatment. All 35 patients presented with non‐fulminant symptoms upon initial assessment. Twenty patients (57.14%) were sensitive, and 15 (42.86%) were refractory to corticosteroids. Compared with controls, 22 patients (62.86%) with ICIaM developed LGE. LVEF decreased in CR ICIaM compared with the CS group and controls. GLSendo (−14.61 ± 2.67 vs. −18.50 ± 2.53, P < 0.001) and GLSepi (−14.75 ± 2.53 vs. −16.68 ± 2.05, P < 0.001) significantly increased in patients with CR ICIaM compared with the CS ICIaM. In patients with CS ICIaM, although GLSepi (−16.68 ± 2.05 vs. −19.31 ± 1.80, P < 0.001) was impaired compared with the controls, GLSendo was preserved. There was no difference in CMR parameters between LGE‐positive and negative groups. LVEF, GLSendo, and GLSepi were predictors of CR ICIaM. When LVEF, GLSendo, and GLSepi were included in multivariate analysis, only GLSendo remained an independent predictor of CR ICIaM (OR: 2.170, 95% CI: 1.189–3.962, P = 0.012). A GLSendo of ≥−17.10% (sensitivity, 86.7%; specificity, 80.0%; AUC, 0.860; P < 0.001) could predict CR ICIaM in the ICIaM cohort. Kaplan–Meier analysis showed that in patients with impaired GLSendo of ≥−17.10%, cardiovascular adverse events (CAEs) occurred much earlier than in patients with preserved GLSendo of <−17.10% (Log‐rank test P = 0.017).ConclusionsCR and CS ICIaM demonstrated different functional and morphological characteristics in different myocardial layers. An impaired GLSendo could be a helpful parameter in early identifying corticosteroid‐refractory individuals in the ICIaM population.
Assessment of the concordance between DN4 scores performed by the patient (self‐assessment) or by the physician (hetero‐assessment) to detect subacute neuropathic postoperative pain
Authors: Adam Frédéric , Chatellier Gilles , Alfonsi Pascal 
Published: January 2024

AbstractBackgroundCentres dedicated to chronic postsurgical pain (CPSP) have been developed, but delays for accessing to it are generally long. Teleconsultation might be a means to facilitate access to care by allowing an initial triage. CPSPs are neuropathic pain in around half of the cases and their diagnosis is mainly based on the score obtained from validated questionnaires. Among them, those requiring a neurological examination (i.e. the Douleur Neuropathique en 4 questions [DN4]) have a better sensitivity and specificity, and should be preferred. However, effectiveness of a remote neurological examination remains to be established. The aim of this observational study is to check during a face‐to‐face consultation if, after a short training, a naïve patient is capable to self‐assess the clinical signs of neuropathic sensations.MethodsThirty patients with suspected neuropathic pain were seen in a face‐to‐face postoperative pain consultation. Before examination, the patient was instructed to fill the DN4 questionnaire, including the neurological examination. Once explanations were given and checked, the patient was left and completed it alone. Then, the pain physician performed the DN4 questionnaire. Inter‐rater reliability between patient and pain physician was assessed for each item and for DN4 score with the Kappa coefficient.ResultsFor each item of the DN4 questionnaire, Kappa coefficients were between 0.74 and 1, and could be considered as excellent. For DN4 ≥ 4, the Kappa coefficient was 0.86.ConclusionsOur results suggest that after a short training, a naïve patient is capable of recognizing and diagnosing symptoms of neuropathic pain.SignificanceOur results suggest that self‐assessment, carried out after brief training and using a simple tool, provides results comparable to those obtained by a specialist physician to diagnose symptoms of neuropathic pain. If the results of the current study are confirmed on a larger scale, self‐assessment will help improve access to specialized chronic pain care by better orienting patients and opening up access to teleconsultations.
Bifidobacterium lactis Probio‐M8 prevents nonalcoholic fatty liver disease in high‐fat diet‐fed rats: The potential role in modulating gut microbiota
Authors: Fan Wen , Tang Kairui , Deng Yuanjun , Zheng Chuiyang , Pan Maoxing , Pi Dajin , Liang Zheng , Zhen Jianwei , Yang Qinhe , Zhang Yupei 
Published: January 2024

AbstractNonalcoholic fatty liver disease (NAFLD) is a major global health problem with few therapeutic options available so far. Accumulating evidence suggests that probiotics have beneficial effects on NAFLD by modulating gut microbiota. Bifidobacterium lactis Probio‐M8 (M8) is a new probiotic strain isolated from human breast milk. The aim of this study was to investigate whether M8 could protect against NAFLD in rats fed a high‐fat diet by modulating gut microbiota. In this study, rats were randomly distributed into four groups: normal diet (ND) group, normal diet plus M8 (ND+M8) group, high‐fat diet (HFD) group, and high‐fat diet plus M8 (HFD+M8) group. Ten weeks later, hepatic morphological changes and biochemical indicators were measured. 16S rDNA sequencing was applied to analyze the gut microbiota alterations. Our results showed that M8 administration effectively improved hepatic steatosis and liver damage in high‐fat diet‐fed rats. 16S rDNA analysis of gut microbiota indicated that M8 could modulate the gut microbiota composition, especially increasing Bifidobacterium and decreasing Bilophila, Lachnoclostridium, GCA‐900066225, and Phascolarctobacterium in high‐fat diet‐fed rats. In conclusion, our findings demonstrated that M8 could protect against NAFLD in rats fed a high‐fat diet, which may be attributed to the modulation of gut microbiota.
Effect of time interval between surgery and the initiation of adjuvant therapy on the oncologic outcomes of early‐stage endometrial cancer
Authors: Suphasynth Yuthasak , La‐Orpipat Thanawut , Saeaib Nungrutai , Janmunee Narumon , Jiamset Ingporn 
Published: January 2024

AbstractObjectiveTo identify the impact of time interval between surgery and initial adjuvant radiotherapy on oncologic outcomes in early‐stage endometrial cancer.MethodsThis retrospective cohort study included patients with stage I/II endometrial cancer who underwent surgical staging and adjuvant therapy at Songklanagarind Hospital from January 1, 2007, to December 31, 2017. Patients were categorized into two groups: TI <6 weeks and TI ≥6 weeks. The effects of TI and clinicopathological factors on recurrence‐free survival (RFS) and overall survival (OS) were analyzed using Cox proportional‐hazards regression.ResultsIn total, 177 patients were enrolled, with 52% receiving adjuvant radiotherapy at <6 weeks (overall median TI 5.7 weeks). The recurrence and death rates were 13% and 10.2%, respectively. The median follow‐up time was 46.6 months. The overall 3‐year RFS and OS rates were 88.2% and 85.2%, respectively. The TI significantly affected the 3‐year RFS (94.4% vs 81.2%; P = 0.008) and 3‐year OS (95.5% vs 83.2%; P = 0.012) in patients with TI <6 and ≥6 weeks, respectively. In multivariate analysis, the depth of myometrial invasion (MI), presence of lymphovascular space invasion, and TI were independent prognostic factors for both RFS and OS. Delaying the TI (≥6 weeks) was significantly associated with a worse RFS (hazard ratio [HR] 3.70; 95% confidence interval [CI]: 1.34–10.22; P = 0.012) and an inferior OS (HR 3.80; 95% CI: 1.23–11.69; P = 0.02).ConclusionA delay in the TI between surgery and the initiation of adjuvant radiotherapy of ≥6 weeks negatively affected the oncologic outcomes in early‐stage endometrial cancer.
Storage‐D: A user‐friendly platform that enables practical and personalized DNA data storage
Authors: Huang Xiaoluo , Cui Junting , Qiang Wei , Ye Jianwen , Wang Yu , Xie Xinying , Li Yuanzhen , Dai Junbiao 
Published: January 2024

AbstractDeoxyribonucleic acid (DNA) has been suggested as a very promising medium for data storage in recent years. Although numerous studies have advocated for DNA data storage, its practical application remains obscure and there is a lack of a user‐oriented platform. Here, we developed a DNA data storage platform, named Storage‐D, which allows users to convert their data into DNA sequences of any length and vice versa by selecting algorithms, error‐correction, random‐access, and codec pin strategies in terms of their own choice. It incorporates a newly designed “Wukong” algorithm, which provides over 20 trillion codec pins for data privacy use. This algorithm can also control GC content to the selected standard, as well as adjust the homopolymer run length to a defined level, while maintaining a high coding potential of ~1.98 bis/nt, allowing it to outperform previous algorithms. By connecting to a commercial DNA synthesis and sequencing platform with “Storage‐D,” we successfully stored “Diagnosis and treatment protocol for COVID‐19 patients” into 200 nt oligo pools in vitro, and 500 bp genes in vivo which replicated in both normal and extreme bacteria. Together, this platform allows for practical and personalized DNA data storage, potentially with a wide range of applications.
Impact of contact force on the lesion characteristics of very high‐power short‐duration ablation using a QDOT‐MICRO catheter
Authors: Yamaguchi Junji , Takigawa Masateru , Goya Masahiko , Martin Claire A. , Negishi Miho , Yamamoto Tasuku , Ikenouchi Takashi , Goto Kentaro , Shigeta Takatoshi , Kawamura Iwanari , Nishimura Takuro , Takamiya Tomomasa , Tao Susumu , Miyazaki Shinsuke , Sasano Tetsuo 
Published: January 2024

AbstractBackgroundLesion size is reported to become larger as contact force (CF) increases. However, this has not been systematically evaluated in temperature‐guided very high‐power short‐duration (vHPSD) ablation, which was therefore the purpose of this study.MethodsRadiofrequency applications (90 W/4 s, temperature‐control mode) were performed in excised porcine myocardium with four different CFs of 5, 15, 25, and 35 g using QDOT‐MICRO™ catheter. Ten lesions for each combination of settings were created, and lesion metrics and steam‐pops were compared.ResultsA total of 320 lesions were analyzed. Lesion depth, surface area, and volume were smallest for CF of 5 g than for 15, 25, and 35 g (depth: 2.7 mm vs. 2.9 mm, 3.0 mm, 3.15 mm, p < .01; surface area: 38.4 mm2 vs. 41.8 mm2, 43.3 mm2, 41.5 mm2, p < .05; volume: 98.2 mm3 vs. 133.3 mm3, 129.4 mm3, 126.8 mm3, p < .01 for all pairs of groups compared to CF = 5 g). However, no significant differences were observed between CFs of 15–35 g. Average power was highest for CF of 5 g, followed by 15, 25, and 35 g (83.2 W vs. 82.1 W vs. 77.1 W vs. 66.1 W, p < .01 for all pairs), reflecting the higher incidence of temperature‐guided power titration with greater CFs (5 g:8.8% vs. 15 g:52.5% vs. 25 g:77.5% vs. 35 g:91.2%, p < .01 for all pairs except for 25 g vs. 35 g). The incidence of steam‐pops did not significantly differ between four groups (5 g:3.8% vs. 15 g:10% vs. 25 g:6.2% vs. 35 g:2.5%, not significant for all pairs).ConclusionsFor vHPSD ablation, lesion size does not become large once the CF reaches 15 g, and the risk of steam‐pops may be mitigated through power titration even in high CFs.
The effect of tenotomy, neurotomy, and dual injury on mouse rotator cuff muscles: Consequences for the mouse as a preclinical model
Authors: Gibbons Michael C. , Silldorff Morgan , Okuno Hiroshi , Esparza Mary C. , Migdal Christopher , Johnson Seth , Schenk Simon , Ward Samuel R. 
Published: January 2024

AbstractA common animal model of muscle pathology following rotator cuff tear (RCT) is a tenotomy of the supraspinatus and infraspinatus, often combined with neurotomy of the suprascapular nerve, which induces a more robust atrophy response than tenotomy alone. However, the utility of this model depends on its similarity to human muscle pathology post‐RCT, both in terms of the disease phenotype and mechanisms of muscle atrophy and fatty infiltration. Given the clinical prevalence of nerve injury is low and the muscular response to denervation is distinct from mechanical unloading in other models, an understanding of the biological influence of the nerve injury is critical for interpreting data from this RCT model. We evaluated the individual and combined effect of tenotomy and neurotomy across multiple biological scales, in a robust time‐series in the mouse supraspinatus. Muscle composition, histological, and gene expression data related to muscle atrophy, degeneration–regeneration, fatty infiltration, and fibrosis were evaluated. Broadly, we found tenotomy alone caused small, transient changes in these pathological features, which resolved over the course of the study, while neurotomy alone caused a significant fatty atrophy phenotype. The dual injury group had a similar fatty atrophy phenotype to the neurotomy group, though the addition of tenotomy did marginally enhance the fat and connective tissue. Overall, these results suggest the most clinically relevant injury model, tenotomy alone, does not produce a clinically relevant phenotype. The dual injury model partially recapitulates the human condition, but it does so through a nerve injury, which is not well justified clinically.
Functional structure and diversity of marine benthos reflect food availability and quality, as evidenced by isotopic diversity
Authors: Szczepanek Marta , Silberberger Marc J. , Kędra Monika 
Published: January 2024

AbstractOrganic matter (OM) resources shape not only the trophic diversity of benthic communities but also the diversity of benthic functional traits. The resulting community structure has a direct effect on many ecosystem processes and functions. Still, the relationship between functional diversity of marine benthos and its trophic diversity in response to the variability of food remains poorly investigated. To explore this relationship we focused on the assessment of various facets of macrobenthic diversity in two temperate coastal areas (southern Baltic Sea) characterized by similar species pool and habitat properties but different OM sources and supplies. We also identified spatio‐temporal patterns of functional structure and assessed, which OM properties had the strongest influence on benthic communities. In our study, functional space dispersion followed isotopic space dispersion, reflecting the positive relationship between food variability and ecological functions of benthic communities. Moreover, functional structure in shallow areas was more location‐specific in comparison to deeper areas, and this was significantly related to OM parameters. Higher OM variability close to the river mouth prevented the community from becoming dominated by one specialized species, whereas limited OM supply at shallow open coast lowered the diversity by enabling stronger competitor to dominate the community. In more offshore areas, local OM variability ceased and hence, communities tended to reflect similar trophic and functional patterns. Our study demonstrates that functional and isotopic diversity approaches can be effectively combined to better understand mechanisms driving benthic community structure and confirm that greater variability in OM supply increases diversity of benthic communities.
Far‐Field Detection of Near‐Field Circular Dichroism Enhancements Induced by a Nanoantenna
Authors: Olmos‐Trigo Jorge , Sugimoto Hiroshi , Fujii Minoru 
Published: January 2024

AbstractNanophotonics can enhance the inherent weak signal exhibited by chiral molecules in circular dichroism (CD) spectroscopy experiments. One mechanism to achieve this CD enhancement is based on increasing the optical chirality produced by nanophotonics antennas in their near‐field, where chiral molecules are placed. However, ascertaining whether this CD enhancement is reached presents significant experimental challenges due to the complexity of measuring near‐field optical chirality. Here, it is demonstrated that a local and single measurement of the degree of circular polarization in the far‐field enables quantifying near‐field CD enhancements. Thereby, based on this far‐field measurement, the optimal response of the antenna is predicted for enhanced chiral sensing. Experimental validation of this near‐to‐far‐field relation is provided for silicon spherical nanoantennas. The experimental results, supported by analytical theory and exact numerical simulations, can be interesting for the experimental characterization of devices capable of enhancing CD from a single far‐field Stokes measurement.
The combination of inorganic phosphate and pyrophosphate 31P‐NMR for the electrodeless pH determination in the 5–12 range
Authors: Carta Paola , Scorciapino Mariano Andrea 
Published: January 2024

AbstractPotentiometry is the primary pH measurement method, but alternatives are sought beyond glass electrodes operative limitations. In nuclear magnetic resonance (NMR) experiments, electrodeless pH sensing is important to track changes along titrations, during chemical reactions or inside compartmentalized environments inaccessible to electrodes, for instance. Although several interesting NMR pH indicators have been already presented, the potential of inorganic phosphate is overlooked, despite its common presence in NMR samples as the buffer main component. Its use for electrodeless pH determination can be expanded by exploiting all its three proton dissociations. This study was aimed at verifying the use of inorganic phosphate 31P chemical shift to sense pH variations, and at exploring the complementary use of pyrophosphate ions to cover a wide pH range. A simple set of equations is presented to utilize both phosphate and pyrophosphate 31P chemical shift in combination for accurate pH determination without a glass electrode over the 5–12 pH range, and without affecting the spectrum of other nuclei. The present study demonstrated an average deviation of 0.09 (maximum <0.2) pH unit from glass electrode measurements. The trimethylphosphate can be used as a suitable chemical shift reference for both 31P and 1H (also 13C), with its hydrolysis being significant only at pH > 12. The method was also demonstrated by determining the pKa of three distinct molecules in a mixture and by comparing the results to those obtained when the glass electrode was used to measure the pH. The approach shown here can be easily tuned to different experimental conditions.
Evaluation of in vitro and in vivo digestibility of potential feed ingredients for juvenile Yellowtail Snapper
Authors: Barreto Alvaro , Arenas Martín , Álvarez-González Alfonso , Suárez-Bautista Jaime , Sánchez Adolfo , Maldonado Carlos , Cuzon Gerard , Gaxiola Gabriela 
Published: January 2024

Abstract

Objective
The carnivorous Yellowtail Snapper Ocyurus chrysurus has the potential for production in aquaculture. However, there is a lack of studies investigating the digestibility of ingredients needed to formulate balanced diets for this species.

Methods
The in vitro protein digestibility of fish meal (FM), poultry by-product meal (PBM), poultry meal (PM), protiblend, canola meal (CM), soybean meal, and wheat gluten was evaluated using the pH-stat technique. The in vivo digestibility was determined by formulating test diets with a 70:30 ratio of the reference diet and the test ingredients.

Result
In terms of in vitro digestibility, FM, PBM, and PM exhibited the highest degree of hydrolysis (DH). Conversely, PBM displayed the lowest apparent digestibility coefficient of protein (ADCprotein) in the in vivo digestibility test. Meanwhile, FM, PM, and CM demonstrated higher values of protein digestibility. Beta regression between ADCprotein and the DH and ash content of the ingredients resulted in a high correlation (R2 = 0.94).

Conclusion
The results of the present study indicate that Yellowtail Snapper efficiently digested and absorbed FM, PM, and CM ingredients, which contain a lower content of ash.