bluuebunny/crossref_metadata_2025_split huggingface dataset:
CXCL9 recombinant adeno-associated virus (AAV) virotherapy sensitizes glioblastoma (GBM) to anti-PD-1 immune checkpoint blockade
Authors: von Roemeling Christina , Yegorov Oleg , Yang Changlin , Klippel Kelena , Russell Rylynn , Trivedi Vrunda , Bhatia Alisha , Doonan Bently , Carpenter Savannah , Ryu Daniel , Grippen Adam , Futch Hunter , Ran Yong , Hoang-Minh Lan , Weidert Frances , Golde Todd , Mitchell Duane 
Published: November 2023

Abstract

The promise of immunotherapy to induce long-term durable responses in conventionally treatment resistant tumors like glioblastoma (GBM) has given hope for patients with a dismal prognosis. Yet, few patients have demonstrated a significant survival benefit despite multiple clinical trials designed to invigorate immune recognition and tumor eradication. Insights gathered over the last two decades have revealed numerous mechanisms by which glioma cells resist conventional therapy and evade immunological detection, underscoring the need for strategic combinatorial treatments as necessary to achieve appreciable therapeutic effects. However, new combination therapies are inherently difficult to develop as a result of dose-limiting toxicities, the constraints of the blood-brain barrier, and the suppressive nature of the GBM tumor microenvironment (TME). GBM is notoriously devoid of lymphocytes driven in part by a paucity of lymphocyte trafficking factors necessary to prompt their recruitment, infiltration, and activation. We have developed a novel recombinant adeno-associated virus (AAV) gene therapy strategy that enables focal and stable reconstitution of the GBM TME with C-X-C motif ligand 9 (CXCL9), a powerful call-and-receive chemokine for cytotoxic T lymphocytes (CTLs). By precisely manipulating local chemokine directional guidance, AAV-CXCL9 increases tumor infiltration by CD8-postive cytotoxic lymphocytes, sensitizing GBM to anti-PD-1 immune checkpoint blockade (ICB). These effects are accompanied by immunologic signatures evocative of an inflamed and responsive TME. These findings support targeted AAV gene therapy as a promising adjuvant strategy for reconditioning GBM immunogenicity given its excellent safety profile, TME-tropism, modularity, and off-the-shelf capability, where focal delivery bypasses the constrains of the blood-brain barrier, further mitigating risks observed with high-dose systemic therapy.
A dual-crosslinking electroactive hydrogel based on gelatin methacrylate and dibenzaldehyde-terminated telechelic polyethylene glycol for 3D bio-printing
Authors: Wang Yulong , Yang Songsong , Cai Heqing , Hu Hailong , Hu Kun , Liu Ruping , Sun Zhicheng , Wei Yen , Han Lu 
Published: November 2023

Abstract
Gelatin was widely used as scaffold materials in 3D bio-printing due to its excellent bioactivity and availability and especially that their arginine-glycine-aspartic acid (RGD) sequences could efficiently promote cell adhesion and proliferation. In this study, physical-chemical double crosslinked gelatin methacrylate-based electroactive hydrogel was prepared via Schiff-base reaction between the free amino groups of gelatin methacrylate (GelMA) and the aldehyde groups of dibenzaldehyde-terminated telechelic polyethylene glycol (DF-PEG), and meanwhile multi-walled carbon nanotubes (MWCNTs) were homogeneously distributed into GelMA solution. The optimized GelMA/DF-PEG composite hydrogel, i.e., 30% GelMA and 25% DF-PEG (G30D25-CNTs), exhibited superior bio-printability. When the content of CNTs was above 4%, the conductivity of G30D25-CNTs hydrogel exceeded 10− 2 S/m, which satisfied the needs of cells for micro-current stimulation. Furthermore, the swelling behavior and degradation ability of G30D25-CNTs electroactive hydrogels were thoroughly evaluated. Thus, the G30D25-CNTs hydrogel with 4% MWCNTs could be considered for further application in electrical stimulation of tissue regeneration such as muscle and cardiac nerve tissue repair.
The microalga Volvox carteri as a cell supportive building block for tissue engineering
Authors: STRICHER Mathilde , Vigneron Pascale , Delbecq Frédéric , Sarde Claude-Olivier , Egles Christophe 
Published: November 2023

Abstract
Background V. carteri f. nagariensis constitutes, in its most simplified form, a cellularized spheroid built around and stabilised by a form of primitive extracellular matrix (ECM).Methods Based on its structure and its ability to support surface cell adhesion most likely induced by the composition of its algal ECM, we have developed a modular approach to soft tissue engineering by compact-stacking of V. carteri–based living building blocks.Results A primary biocompatibility assessment demonstrated the algal suspension cytocompatibility, its histogenesis promoting properties, and that it did not induce an inflammatory response in vitro. These results allowed us to consider the use of such algal suspension for soft tissue augmentation and to initiate the study of its in vivo biocompatibility. V. carteri exhibited cellular fate-directing properties, causing fibroblasts to take on an alkaline phosphatase+ stem-cell-like phenotype and both human adipose-derived stem cells and mouse embryonic stem cells to differentiate into preadipocytes to adipocytes. The ability of V. carteri to support histogenesis and adipogenesis was also observed in vivo by subcutaneous tissue augmentation of athymic mice, highlighting the potential of V. carteri to support or influence tissue regeneration.Conclusions Our conclusion present for the first time V. carteri as an innovative and inspiring biomaterial for tissue engineering and soft tissue regeneration. Its strategies in terms of shape, structure and composition can be central in the design of a new generation of bio-inspired heterogeneous biomaterials recapitulating more appropriately the complexity of the body tissues when guiding their regeneration.
Risk assessment of antimony-arsenic contaminated soil remediated using zero-valent iron at different pH values combined with freeze-thaw cycles
Authors: Hei Erping , He Mingjiang , Zhang Enze , Yu Hua Yu , Chen Kun Chen , Qin Yusheng Qin , Zeng Xiangzhong , Zhou Zijun , Fan Hongzhu , Yuxian Shangguan , Wang Luying 
Published: November 2023

Abstract
Soil in mining wastelands is seriously polluted with heavy metals. Zero-valent iron (ZVI) is widely used for remediation of heavy metal-polluted soil because of its excellent adsorption properties; however, the remediation process is affected by complex environmental conditions, such as acid rain and freeze-thaw cycles. In this study, the effects of different pH values and freeze-thaw cycles on remediation of antimony (Sb)- and arsenic (As)-contaminated soil by ZVI were investigated in laboratory simulation experiments. The stability and human health risk of the remediated soil were evaluated. The results showed that different pH values coupled with freeze-thaw cycles could promote stabilization remediation of Sb- and As-contaminated soil by ZVI. Among them, ZVI significantly reduced the leaching toxicity and bioavailability of Sb and As in soil (both about 60.00%) after 32 rounds of freeze-thaw cycle treatment under water sample conditions of pH=1 (F1). However, Compared with soil not treated with freeze-thaw cycles, freeze-thaw cycles increased the leaching concentration of unstable forms of As. Bioavailability calculations based on Sb and As in remediated soil that alkaline soil there were no health or carcinogenic risks for children and adults, but the health risks and carcinogenic risks of Sb in acidic soil remained high for children. These findings provide a theoretical basis for the remediation of Sb- and As-contaminated soil using ZVI, and also highlight the importance of considering pH and freeze-thaw conditions in the remediation process to ensure effective and safe treatment.
Data-driven prediction of continuous renal replacement therapy survival
Authors: Bui Alex , Zamanzadeh Davina , Feng Jeffrey , Petousis Panayiotis , Vepa Arvind , Sarrafzadeh Majid , Karumanchi S. , Kurtz Ira 
Published: November 2023

Abstract

Continuous renal replacement therapy (CRRT) is a form of dialysis prescribed to severely ill patients who cannot tolerate regular hemodialysis. However, as the patients are typically very ill to begin with, there is always uncertainty as to whether they will survive during or after CRRT treatment. Because of outcome uncertainty, a large percentage of patients treated with CRRT do not survive, utilizing scarce resources and raising false hope in patients and their families. To address these issues, we present a machine-learning-based algorithm to predict if patients will survive after being treated with CRRT. We use information
extracted from electronic health records from patients who were placed on CRRT at multiple institutions to train a model that predicts CRRT survival outcome; on a held-out test set, the model achieved an area under the receiver operating curve of 0.929 (CI=0.917-0.942). Feature importance, error, and subgroup
analyses identified consistently, mean corpuscular volume as a driving feature for model predictions. Overall, we demonstrate the potential for predictive machine-learning models to assist clinicians in alleviating the uncertainty of CRRT patient survival outcomes, with opportunities for future improvement through further data collection and advanced modeling.
Optimum design parameters for double concave friction pendulum system considering ground motion characteristics and friction heating
Authors: Li Jiaxi , Tan Ping , Yang Kui , Liu Jingjing , Yamazaki Shinsuke , Yamada Satoshi 
Published: November 2023

Abstract

This paper addresses effectiveness limitations in the design of double concave friction pendulum systems (DCFPS) due to the absence of considering the interrelation between design parameters, ground motions (GM) characteristics, friction heating and seismic responses. By systematically evaluating the optimum design parameters for DCFPS under different strong GM classifications considering the effect of friction heating, it contributes a comprehensive understanding of the efficacy of DCFPS in vibration control. In this study, an analytical model for DCFPS in the SDOF structure is established, providing a precise characterization of the effect of friction heating and loading conditions on the responses. Based on this, the friction coefficient considering the influence of friction heating under strong GMs is applied in the seismic design. Subsequently, from the perspective of GM generation, strong GM records are categorized based on earthquake magnitude, distance-to-fault and soil conditions to indicate their magnitudes and spectral attributes. Then, the optimal design parameters are investigated for different GM classifications considering the effect of friction heating and response restrictions using response magnitude as criterion, and the effect of GM characteristics on the response and design parameters of DCFPS is investigated. It was found that, the consideration of residual displacement restriction and friction heating shows critical influence on the optimum selection of design parameters. Furthermore, the optimum friction coefficient is highly related to distance-to-fault, while the optimum range of isolation period is minimally influenced by GM characteristics. On top of these, a performance-based design method of FPS considering three performance criteria corresponding to three GM intensity levels is proposed, providing an effective method for mitigating the vibration of FPS under GMs.
Flight test results for the Microgravity Active Vibration Isolation System employed in the Fluid Physics Research Rack on-board the Chinese Space Station
Authors: Liu Wei , Gao Yang , Zhang Long , Zou Tianji , Yu Mengxi , Zheng Tuo 
Published: November 2023

Abstract
The Fluid Physics Research Rack (FPR) is a research platform employed on-board the Chinese Space Station for conducting microgravity fluid physics experiments. The research platform includes the Microgravity Active Vibration Isolation System (MAVIS) for isolating the FPR from disturbances arising from the space station itself. In addition, the MAVIS includes a microgravity operating mode that provides an environment with a controllable acceleration on the order of one millionth of the gravitational acceleration of the earth at sea level (i.e., µg0, where g0 = 9.80665 m/s2), and a vibration excitation operating mode that provides an environment with controllable vibrational acceleration signals of specific amplitudes in the frequency range of 0.01–10 Hz. The MAVIS is a structural platform consisting of a stator and floater that are monitored and controlled with non-contact electromagnetic actuators, high-precision accelerometers, and displacement transducers. The stator is fixed to the FPR, while the floater serves as a vibration isolation platform supporting payloads, and is connected with the stator only with umbilicals that mainly comprise power and data cables. However, the umbilicals have some stiffness that provides pathways for the transfer of disturbances from the stator to the floater. Therefore, the controller was designed with a correction for the umbilical stiffness to minimize the effect of the umbilicals on the vibration isolation performance of the MAVIS. In-orbit test results of the FPR demonstrate that the MAVIS was able to achieve a microgravity level of 1–30 µg0 in the frequency range of 0.01–125 Hz under the microgravity mode, and disturbances with a frequency greater than 2 Hz are attenuated by more than 10-fold. Under the vibration excitation mode, the MAVIS generated a minimum vibration acceleration of 0.4091 µg0 at a frequency of 0.00995 Hz and a maximum acceleration of 6253 µg0 at a frequency of 9.999 Hz. Therefore, the MAVIS provides a highly stable environment for conducting microgravity experiments, and promotes the development of microgravity fluid physics.
Ion Mobility-Tandem Mass Spectrometry of Mucin-type O-Glycans
Authors: Pagel Kevin , Bechtella Leïla , Chunsheng Jin , Fentker Kerstin , Ertürk Güney , Safferthal Marc , Polewski Łukasz , Götze Michael , Graeber Simon , Struwe Weston , Mall Marcus , Mertins Philipp , Karlsson Niclas 
Published: November 2023

Abstract
The dense O-glycosylation of mucins plays an important role in the defensive properties of the mucus hydrogel. Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn’s disease. The inherent complexity of glycans and the diversity in the O-core structure constitute fundamental challenges for the analysis of mucin-type O-glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying O-glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is drastically reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map O-glycosylation features associated with disease.
How many blood pressure measurements should we take in the office?
Authors: Ozkan Gulsum , Ulusoy Sukru , Yılmaz Rahmi , Değer Müge , Derici Ülver , Arınsoy Turgay , Erdem Yunus 
Published: November 2023

Abstract
Background: No consensus has emerged among different guidelines concerning how many blood pressure (BP) measurements should be performed at office visits in the diagnosis of hypertension (HT). The purpose of this study was to examine the compatibility of various multiple average office BP measurements and 24-h BP monitoring (ABPM) in patients follow-up in the Cappadocia HT cohort.
Methods: 1158 office BP measurements by 207 patients were examined. Five BP measurements were taken at each office visit. The results were then classified as G1 (average of the 1st and 2nd BP measurements), G2 (average of the 2nd and 3rd), G3 (average of the 2nd, 3rd, and 4th), G4 (average of the 2nd, 3rd, 4th, and 5th), and G5 (average of five measurements). Compatibility between the average values in the groups and concomitant 24-h ABPM data was examined.
Results: While a significant difference was observed between daytime 24-h ABPM SBP and G1 (p=0.002), no difference was found the other groups. Office DBP approached the daytime 24-h ABPM values as the number of measurements in the five groups increased, although average office DBP data in all groups were higher than daytime 24-h ABPM DBP (p 0.000 for all).
Conclusion:In the light of our study results, we recommend that three office BP measurements be performed and that the average of the 2nd and 3rd measurements be used for SBP, while in terms of DBP, we recommend that as many measurements as possible be taken without the 1st value being included in the average.
The effect of discordance between embryologist and pathologist on sperm retrieval rates in patients with non-obstructive azoospermia undergoing mTESE
Authors: Sahin Mehmet , CAN OSMAN , Keskin Emin , Filtekin Yigit , Canat Lutfi 
Published: November 2023

Abstract
This study investigates predictive factors, specifically the consistency between embryologist and pathologist, influencing sperm retrieval rates in microdissection testicular sperm extraction for non-obstructive azoospermia. The research involved 91 patients aged 20 to 47, who applied to our Reproductive Technology center from July 2020 to June 2023. Patient data, including demographics, physical exams, lab tests, radiological assessments, genetic analyses, medical histories, and histopathological results, were retrospectively collected. Different than literature findings, cases were identified where histopathology showed spermatozoa, yet microdissection testicular sperm extraction yielded unsuccessful sperm retrieval. The Johnsen score was categorized into three groups: spermatozoa found (Group I), maturation arrest (Group II), and no spermatogenesis (Group III). In Group I, despite pathologist-identified spermatozoa, only 66.6% were deemed successful sperm retrieval by the embryologist. Overall sperm retrieval rates were lower than expected at 39.56%. A logistic regression model was employed to determine the independent predictors of positive sperm retrieval. In this analysis, FSH levels, testicular volume, histopathological findings, and alcohol consumption emerged as significant predictive factors for the outcome of sperm retrieval. As a result, in addition to these patient-related factors, the consistency between the pathologist and histologist is also important.
The mechanism of element inhomogeneity in TW-DED-arc fabricated γ-TiAl Alloy
Authors: Xin Jianwen , Li Fang , Wu Dongsheng , Shen Chen , Komen Hisaya , Tanaka Manabu , Hua Xueming 
Published: November 2023

Abstract
The twin-wire directed energy deposition-arc (TW-DED-arc) method is a low-cost and efficient in-situ alloying process for producing γ-TiAl alloy, a new generation material for aero-engine blades. Its characteristic of “twin-wire-one-drop” can successfully avoid the phenomenon of discordant melting and ineffective mixing. In this study, the mixing effect of “twin-wire-one-drop” was analysed, and droplets of different diameters were used for fabricating Ti52Al48 walls. It was found that the mixing effect in the droplet was great, but there were still local unmixed areas, and a completely uniform Ti52Al48 wall could be obtained by using small droplets mode. Meanwhile, incompletely mixing regions with composition difference greater than 5% appeared in many places on the sides of the Ti48Al wall in huge droplet mode. A numerical model is established to simulate the mixing process after the droplet enters the molten pool. It is found that the secondary droplets generated in huge droplet mode is the main reason for the element inhomogeneity phenomenon. Therefore, keeping the droplet interval short and uniform is beneficial to the element in-situ alloying.
Phytochemical analysis of Stevia rebaudiana using HPLC and GC-MS
Authors: Gunasena M.D.K.M. , Senarath W.T.P.S.K. 
Published: November 2023

Abstract

Stevia rebaudiana is a medicinal plant rich in important phytochemicals including steviol glycosides. Its’ leaves are the source of non-caloric sweet steviol glycosides and stevioside is the main type. In this study leaves of naturally grown, tissue cultured plants and callus extracts of S. rebaudiana were analyzed using HPLC and GC-MS to determine the possibility of obtaining stevioside and other important phytochemicals through in vitro technology. Phytochemical extraction was done using methanol, evaporated using a rotary evaporator and re-dissolved in acetonitrile: deionized water (3:7) ratio solvent mixture. Stevioside contents were analyzed using HPLC system with C18 column. Methanolic extracts of naturally grown plants, tissue cultured plants and calli were screened using GC-MS and compared. According to HPLC analysis, leaves of tissue cultured plants contained highest amount of stevioside content (11.462 ± 0.03 mg/mL) followed by naturally grown plants and calli. Twenty seven important phytochemicals were identified by GC-MS analysis and out of those fourteen compounds were found to be common in all three samples. Results revealed that in vitro cultures can be used as the better alternative to overcome limitations in conventional propagation and can be used to obtain stevioside and other important phytochemicals instead of natural plants. GC-MS analysis of Stevia extracts revealed the presence of terpenes, fatty acids, aldehydes with biological activities such as antimicrobial, anticancer, anti-inflamatory, antioxidant, antimalarial and etc. Bioactivity of those phytochemicals showed the possible use of Stevia extracts in pharmaceutical, food and perfume industries.
Exploring patient-related factors behind racial inequities in buprenorphine treatment for opioid use disorder in primary care
Authors: Kung Sunny , Casey Sarah , Regan Susan , McGovern Sydney , Sawyer Samantha M. , Wakeman Sarah E. 
Published: November 2023

Abstract
From 2019 to 2020, overdose death rates increased 39% among Black Americans nationally. The goal of this study is to understand patient-related etiologies behind racial inequities in buprenorphine treatment for opioid use disorder (OUD) in primary care patients. We surveyed adults with primary care providers (PCPs) in practices at an integrated healthcare system in Massachusetts where buprenorphine was prescribed. 428 adults were randomly sampled, 278 confirmed to have OUD via chart review, and 33 patients completed the survey. 36.4% (n=12) identified as LatinX, 33.3% (n=11) identified as Black, 27.3% (n=9) as white. 82% (n=27) reported having ever been prescribed buprenorphine for OUD [100% (n=9) of white respondents, 75% (n=9) of Latinx respondents, 73% (n=8) of Black respondents]. A majority (n=23) of respondents preferred to get their OUD treatment at their PCP’s office [82% (n=9) of Black respondents, 75% (n=9) of LatinX respondents, and 56% (n=5) of white respondents]. Most Black and Latinx patients preferred to get their OUD treatment at their PCP’s office although only half have ever discussed buprenorphine with their PCP. Black and Latinx patients placed more importance on having healthcare staff who are from and know about different racial/ethnic backgrounds compared to white patients. None of the respondents reported that having their PCP share or understand their racial/ethnic background would improve buprenorphine access. Respondents suggested that hiring more staff and clinicians who are Black and/or Latinx, treating people with SUD compassionately, and offering financial assistance would better meet the needs of Black and Latinx patients.