bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signal Peptide Selection for the Efficient Periplasmic and Secretive Expression of Recombinant Brazzein in Escherichia Coli
Authors: Muminov Muzaffar , Ermatova Khusnora , Sohibnazarova Khonsuluv , Dalimova Dilbar , Turdikulova Shahlo 
Published: December 2023

Background:
The high production cost and difficulty of functional expression of brazzein are the limiting factors, making the development of inexpensive, scalable technologies critical for their successful implementation in the market. Secretory expression allows functional expression of the S-S bond-rich proteins and facilitates the purification procedure, resulting in lower processing costs. However, extensive screening and optimization of multiple signal peptides are required to ensure the successful secretion of recombinant proteins.

Objective:
We studied the expression of the minor type of brazzein using 21 different signal peptides in Escherichia coli and investigated their ability to direct the target protein into periplasmic space and culture medium.

Methods:
The synthetic genes were cloned into the pSEVA234 vector under the inducible Trc promoter and initial micro-scale expression analysis was conducted at two distinct conditions followed by scale-up and purification of the selected signal peptides with secretive abilities.

Results:
Two signal peptides led to the secretion of the target protein. The yields of the target protein for MalE_Brazzein and HstI_Brazzein in the periplasm were 11.33 mg/L and 52.33 mg/L, and those in the culture media were 3.975 mg/L and 7.73 mg/L, respectively.

Conclusion:
This study will provide insights into the identification of optimal signal peptides for secretive brazzein expression in E.coli and demonstrate that the abovementioned two signal peptides can be used for successful extracellular production of the target protein in this host.
Productivity of Cherry Tomato Cultivars as Influenced by Watering Capacities and Microclimate Control Designs
Authors: Kusumiyati Kusumiyati , Ahmad Farhan , Khan Muhammad Rabnawaz , Soleh Mochamad Arief , Sundari Ristina Siti 
Published: December 2023

Background:
Cherry tomatoes remain relatively expensive due to limited public knowledge, few producers, and lower productivity rates in Indonesia. Although cherry tomatoes are increasingly popular in modern marketplaces, they are still uncommon in traditional markets.
Cherry tomatoes have an enormous economic impact on many regions, particularly Indonesia, in the broader agricultural environment. Their cultivation, however, may be impacted by problems with water supply and climatic regulation. By evaluating how various watering capacity and microclimate control strategies affect cherry tomato productivity, this study seeks to address these challenges. The research is anticipated to offer farmers and agricultural practitioners useful information to improve cherry tomato output in response to market demands and sustainability concerns.

Objectives:
The objective of this study is to determine the effect of cultivars on the growth, yield, and quality of cherry tomatoes.

Methods:
The research is designed to determine the effect of each cultivar (Ruby, Fortesa), watering capacities (100% ETc, 75% ETc, 50% ETc), and microclimate control designs (Greenhouse, Rain shelter, Screen house) on the growth, yield, and quality of cherry tomatoes.

Results:
The cultivar Fortesa produced the highest possible fruit, biological yield per plant, maximum marketable fruit output, greater growth index, maximum fruit firmness, and water use efficiency. The cultivar Ruby performed better in terms of root-shoot ratio and fruit elasticity. The greenhouse produced the highest possible fruit and biological yield per plant, marketable fruit yield, growth index, leaf chlorophyll content, fruit firmness, elasticity and water use efficiency. In the watering capacities treatment condition, 100% ETC was more significant for parameters, such as marketable fruit yield, root-shoot ratio, growth index, leaf chlorophyll content, fruit firmness, and elasticity.

Conclusion:
Growing cherry tomato cultivar Fortesa in a greenhouse using a watering capacity of 100% ETc under the results is recommended. These conditions have improved fruit quality, making them favorable options for successful cherry tomato cultivar production.
Improvements in Adolescents’ Disordered Eating Behaviors in a Collaborative Care Digital Mental Health Intervention: Retrospective Observational Study
Authors: Huffman Landry Goodgame , Lawrence-Sidebottom Darian , Beam Aislinn Brenna , Parikh Amit , Guerra Rachael , Roots Monika , Huberty Jennifer 
Published: December 2023

Background
Young people today are exhibiting increasing rates of disordered eating behaviors, as well as eating disorders (EDs), alongside other mental and behavioral problems such as anxiety and depression. However, limited access to mental health care means that EDs, disordered eating behaviors, and comorbid mental health problems are often underdiagnosed and undertreated. Digital mental health interventions (DMHIs) offer accessible and scalable alternatives to traditional treatment modalities, but their effectiveness has not been well established among adolescents with EDs and disordered eating behaviors.

Objective
This study uses data from a collaborative care pediatric DMHI to determine whether participation in a DMHI is associated with a reduction in adolescents’ disordered eating behaviors.

Methods
Adolescent members in care with Bend Health Inc completed the SCOFF questionnaire at baseline (before the start of care) and approximately every month during care to assess disordered eating behaviors. They also completed assessments of mental health symptoms at baseline. Member characteristics, mental health symptoms, and disordered eating behaviors of adolescents with elevated SCOFF scores at baseline (before the start of care) were compared to those of adolescents with nonelevated SCOFF scores at baseline. Members participated in web-based coaching or therapy sessions throughout the duration of mental health care.

Results
Compared to adolescents with nonelevated SCOFF scores (n=520), adolescents with elevated SCOFF scores (n=169) were predominantly female and exhibited higher rates of elevated anxiety and depressive symptoms. SCOFF scores decreased over time in care with the DMHI for 61.4% (n=70) of adolescents with elevated SCOFF scores, and each additional month of participation was associated with greater improvements in disordered eating behaviors (F1,233=72.82; P<.001).

Conclusions
Our findings offer promising preliminary evidence that participation in mental health care with a collaborative care DMHI may be beneficial in the reduction of disordered eating symptoms in adolescents, including those who are experiencing comorbid anxiety and depressive symptoms.
Warisan Budaya dalam Konteks Standar Internasional: Penjagaan Warisan Budaya Untuk Pembangunan Berkelanjutan
Authors: Ramadhan Muhammad Fadil , Adi Prasetijo  
Published: December 2023

In the era of globalization, infrastructure and economic development have become the primary focus of countries worldwide. While these projects have the potential to improve quality of life and economic growth, they can also have negative impacts on the environment and cultural heritage. This article discusses the role of international lending institutions, such as Multilateral Development Banks (MDBs), in supporting the preservation of cultural heritage in the context of sustainable development. Literature review is used as the research method to inspect the approaches and efforts made by these institutions in identifying, protecting, and conserving cultural heritage in development projects. Through improved understanding and collaboration among these institutions, businesses, and local communities, the preservation of cultural heritage can become an integral part of efforts towards inclusive and sustainable development. Awareness of the importance of unexpected discovery handling procedures is also crucial to address challenges that may arise during project implementation. With the right measures in place, cultural heritage can be preserved for future generations in sustainable development.
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Dalam era globalisasi, pembangunan infrastruktur dan ekonomi menjadi fokus utama negara-negara di seluruh dunia. Meskipun proyek-proyek ini memiliki potensi untuk meningkatkan kualitas hidup dan pertumbuhan ekonomi, mereka juga dapat berdampak negatif pada lingkungan dan warisan budaya. Artikel ini membahas peran lembaga internasional peminjam dana, seperti Multilateral Development Bank (MDB), dalam mendukung pelestarian warisan budaya dalam konteks pembangunan berkelanjutan. Studi literatur digunakan sebagai metode penelitian untuk menelusuri upaya yang dilakukan oleh lembaga-lembaga ini dalam mengidentifikasi, melindungi, dan melestarikan warisan budaya dalam proyek-proyek pembangunan. Melalui pemahaman dan kolaborasi yang lebih baik antara lembaga-lembaga ini, pelaku usaha, dan komunitas lokal, pelestarian warisan budaya dapat menjadi bagian integral dari upaya menuju pembangunan berkelanjutan yang inklusif dan berkelanjutan. Kesadaran akan pentingnya prosedur penanganan temuan tak terduga juga penting untuk mengatasi tantangan yang mungkin muncul selama pelaksanaan proyek. Dengan langkah-langkah yang tepat, warisan budaya dapat dilestarikan untuk generasi mendatang dalam pembangunan yang berkelanjutan.
CONTROL DIFUSO PARA PINZA ROBÓTICA BLANDA ORIENTADA A OBJETOS NO RÍGIDOS
Authors: Jiménez Moreno Robinson , Martínez Baquero Javier Eduardo , Agudelo Varela Oscar 
Published: December 2023

El presente artículo expone el diseño de un efector robótico construido con sensórica interna en material flexible. Basados en el agarre bioinspirado de objetos delgados típicamente no rígidos realizados con dos dedos por os humanos, se establecen las características del modelo 3 que sirve de base para la impresión de este, incluyendo espacio interno para una flexoresistencia que permita identificar el porcentaje de flexión para agarre, mediante el efector. Se diseña un controlador difuso para control del efector y dada la tolerancia del sensor se emplea un sistema de inferencia difusa Mamdani tipo-2. Los resultados muestran un agarre adecuado que permite obtener un error de estado estacionario cercano a cero, permitiendo agarrar objetos delgados como un pañuelo o una pieza de papel higiénico.
ANALISIS KEHILANGAN AIR IRIGASI DI SALURAN KR 2A KIRI DESA PURWODADI KECAMATAN TRIMURJO KABUPATEN LAMPUNG TENGAH
Authors: Riswan Riedo , Prawati Eri , Rolia Eva 
Published: December 2023

Air irigasi merupakan sumberdaya pertanian yang sangat strategis. Agar jaringan irigasi tersebut dapat digunakan sesuai dengan fungsinya, maka diperlukan adanya pengelolaan jaringan irigasi yang efektif dan efesien. Pengelolaan jaringan irigasi akan mempengaruhi sistem pemberian air pada petak-petak sawah dan tingkat pelayanan irigasi yang diterima oleh petani. Pada musim kemarau kebutuhan air di areal pertanian Desa Purwodadi belum dapat terpenuhi, sehingga sangat berpengaruh pada hasil produksi petani. Hal tersebut terjadi kemungkinan dikarenakan pengelolaan air irigasi dan pendistribusiannya masih kurang merata.
Berdasarkan pada pernyataan di atas, maka peelitian ini dilakukan dengan maksud untuk mengkaji pemanfaatan dan penyaluran air irigasi pada areal persawahan tersebut. Areal persawahan yang dimaksud dialiri oleh saluran irigasi Kr 2A Kiri di Desa Purwodadi, Kecamatan Trimurjo Kabupaten Lampung Tengah. Penelitian ini dilakukan secara bertahap, langkah-langkah dalam pengumpulan data yaitu melakukan survei lokasi dengan mengukur dimensi saluran dan dimensi pintu air, melakukan penelitian dan perhitungan jumlah debit air yang masuk pada pukul 08.00 WIB, yang dilakukan adalah melihat tinggi air lalu diukur ketinggiannya, menghitung jumlah kebutuhan air di sawah dengan memperhitungkan faktor kehilangan air akibat faktor jarak, menghitung toal jumlah kehilangan air pada saluran yang diakibatkan oleh faktor rembesan, kesalahan operasional pintu air dan faktor jarak saluran ke petak sawah, lalu yang terakhir adalah menghitung tingkat efisiensi penyaluran air irigasi.
Dapat disimpulkan bahwa nilai kehilangan air pada saluran A adalah sebesar 0,10 m3/d dengan nilai efisiensi 84,76%, saluran A merupakan saluran yang memiliki persentase efisiensi terendah daripada saluran B dan C. Dimana saluran B memiliki nilai kehilangan air sebesar 0,05 m3/d dan nilai persentase efisiensi sebesar 91,84% sedangkan saluran C memiliki nilai kehilangan air sebesar 0,01 m3/d dengan nilai persentase efisiensi sebesar 98,98%.Faktor yang menyebabkan kehilangan air pada saluran SA, saluran SB, dan saluran SC adalah karena sebagian saluran masih belum ditalut dan masih banyaknya tanaman-tanaman liar yang tumbuh di sepanjang saluran irigasi.
Carbon dioxide laser ablation for treatment of limbal squamous cell carcinoma in horses
Authors: English Robert V. , Nasisse Mark P. , Davidson Michael G. 
Published: December 2023

Summary
Limbal squamous cell carcinoma in 4 horses was treated successfully, using carbon dioxide laser ablation. Tumors were removed, with minimal to no postoperative inflammation or discomfort to the horses. Carbon dioxide laser ablation represents a promising new option in the treatment of limbal squamous cell carcinoma in horses.
Prevalence of antithyroglobulin antibodies detected by enzyme-linked immunosorbent assay of canine serum
Authors: Beale Karin Muth , Halliwell Richard E. W. , Chen Chao L. 
Published: December 2023

Summary
Antithyroglobulin antibody (ata) values were higher in dogs with low total serum thyroxine (T4) and triiodothyronine (T3) values than in dogs with T3 and T4 values within reference value limits. In the population studied, Doberman Pinschers were predisposed to the development of ata; there was no sex predilection for development of ata. Antithyroglobulin antibodies and thyroid status were evaluated in 2 groups of healthy dogs (n = 30) and in 470 canine serum samples submitted for T3 and T4 value determination. Antithyroglobulin antibodies were evaluated by elisa, and thyroid status was evaluated by measurement of total serum T3 and T4 by radioimmunoassay.
Electrolyte abnormalities associated with diarrhea in rhesus monkeys: 100 cases (1986-1987)
Authors: George Jeanne Wright , Lerche Nicholas W. 
Published: December 2023

Summary
Serum electrolyte values from 100 rhesus monkeys with diarrhea were reviewed. The most frequent finding was hyponatremia (88%), with hypochloremia next most frequently detected (80%). Metabolic acidosis was less common (59%) and usually associated with high anion gap values. Associations between electrolyte abnormalities and age, housing, or case outcome were not found. Bacteriologic culturing was performed on fecal specimens from 90 monkeys. Campylobacter coli or C jejuni alone was isolated from 42 (46.7%) specimens, C coli and Shigella flexneri were isolated from 25 (27.8%) specimens, and S flexneri alone was isolated from 6 (6.7%) specimens. A pathogen was not isolated from 17 (18.9%) specimens. Hyponatremia, hypochloremia, acidosis, and high anion gap values were most severe in monkeys infected with Campylobacter sp, either alone or with concurrent S flexneri infection. Serum sodium concentrations < 132 mEq/L and serum Cl concentrations < 93 mEq/L were consistently associated with Campylobacter sp infection.
Assessment of survey radiography and comparison with x-ray computed tomography for detection of hyperfunctioning adrenocortical tumors in dogs
Authors: Voorhout George , Stolp Ruurd , Rijnberk Ad , van Waes Paul F.G.M. 
Published: December 2023

Summary
Results of abdominal survey radiography and x-ray computed tomography (CT) were compared in 13 dogs with hyperadrenocorticism histologically attributed to adrenocortical tumors. X-ray computed tomography enabled accurate localization of the tumor in all 13 dogs. Apart from 2 poorly demarcated irregular-shaped and mineralized carcinomas, there were no differences between adenoma (n=3) and carcinoma (n= 10) on CT images. In 1 dog, invasion of the caudal vena cava by the tumor was suggested on CT images and was confirmed during surgery. Suspicion of adhesions between tumors of the right adrenal gland and the caudal vena cava on the basis of CT images was confirmed during surgery in only 2 of 6 dogs.
Survey radiography allowed accurate localization of the tumor in 7 dogs (4 on the right side and 3 on the left). In 6 of these dogs, the tumor was visible as a well-demarcated soft tissue mass and, in the other dog, as a poorly demarcated mineralized mass. The smallest tumor visualized on survey radiographs had a diameter of 20 mm on CT images. Six tumors with diameter ≤ 20 mm were not visualized on survey radiographs. In 1 of these dogs, a mineralized nodule was found in the left adrenal region, without evidence of a mass.
In a considerable number of cases, survey radiography can provide presurgical localization of adrenocortical tumors in dogs with hyperadrenocorticism; CT is redundant in these instances. In the absence of positive radiographic findings, CT is valuable for localization of adrenocortical tumors.
Drug therapy in cats: Recommended dosing regimens
Authors: Boothe Dawn Merton 
Published: December 2023

Summary
The goal of this series of articles has been to provide a comprehensive review of the literature regarding recommended dosing regimens, therapeutic indications and contraindications, and potential side effects of drugs used in cats. In this fourth and last article, the available information regarding dosage regimens in cats has been consolidated in tabular form to facilitate an effective and rational approach to the pharmacologic prevention and treatment of a variety of feline medical disorders.
Transendoscopic contact neodymium:yttrium aluminum garnet laser correction of epiglottic entrapment in standing horses
Authors: Tulleners Eric P. 
Published: December 2023

Summary
Fifty-seven Standardbred and 44 Thoroughbred racehorses and 1 Thoroughbred polo mare with primary clinical signs of exercise intolerance or respiratory tract noise or combined exercise intolerance and respiratory tract noise were referred for laser correction of epiglottic entrapment. Significantly (P < 0.001) more Standardbred than Thoroughbred racehorses were affected, compared with the observed hospital population during the same period. At referral, 14 horses did not have evident epiglottic entrapment and were returned to exercise without development of entrapment after treatment, which consisted of 1 week of rest and administration of anti-inflammatory medication.
In 88 standing horses under sedation and topical anesthesia, epiglottic entrapment was corrected transendoscopically by use of a contact neodymium:yttrium aluminum garnet laser. In these 88 horses, 98% of entrapments were persistent, 92% were thick, 97% were wide, and 45% were ulcerated. Thirty-one percent of the horses had endoscopic evidence of epiglottic hypoplasia, and 8% had deviated epiglottic axis.
Complete correction was achieved in 97% of the horses, Persistent dorsal displacement of the soft palate in 1 horse and severe epiglottic hypoplasia with thick, chronic entrapping membranes in 2 horses precluded successful transendoscopic correction with the horses in standing position. Most horses were treated onan outpatient basis, and all were able to be returned to exercise after 7 to 14 days of rest and treatment with anti-inflammatory medication.
Entrapment recurred in 4 horses (5%), 3 of which had hypoplastic epiglottis. Dorsal displacement of the soft palate developed after surgery in 9 horses (10%) and continued in 4 horses (5%) that had displaced soft palate before surgery. All these horses had epiglottic hypoplasia.
Laser correction of epiglottic entrapment in standing horses was safe, well tolerated, and effective. Laser surgery was an alternative to conventional surgery, and eliminated the need for general anesthesia and laryngotomy. It also reduced convalescence and postoperative complications.
Lack of detectable blood groups in domestic ferrets: Implications for transfusion
Authors: Manning Dean D. , Bell Judith A. 
Published: December 2023

Summary
Evidence of blood groups in domestic ferrets was sought by testing serum samples for naturally acquired or experimentally induced erythrocyte antibodies. All sera were tested for ability to cause direct agglutination, antiglobulin-enhanced (Coombs test) agglutination, or lysis.
Examination of 212 randomly paired combinations of ferret serum and erythrocytes produced no evidence of naturally acquired blood group antibodies. Six pairs of ferrets were reciprocally transfused twice, 34 days apart, with 6-ml quantities of anticoagulated blood. All were tested 21 days after the first transfusion, as well as 10 and 30 days after the second transfusion; erythrocyte antibodies were not detected. Four additional pairs of ferrets were reciprocally inoculated sc with a series of six 1.25-ml quantities of blood in Alsever solution, administered over a 3-week period, and tested 8 days after the last injection; again, erythrocyte antibodies could not be detected.
These observations suggest that blood groups of the kind in human beings and other mammals either do not exist in domestic ferrets or represent antigen systems too weak to elicit measurable responses under the reported conditions. It appears, therefore, that transfusion in this species poses little clinical risk, even without crossmatching.
Economic analysis of an epizootic of pseudorabies and subsequent production following the institution of a vaccination program in a Pennsylvania swine herd
Authors: Parsons T. D. , Pitcher P. M. , Johnstone C. 
Published: December 2023

Summary
The economic impact of pseudorabies was examined in a commercial swine herd. At the onset of clinical signs, a modified-live virus vaccine was administered to the sow herd and repeated at 3-month intervals. According to production data from the 320-sow farrow-to-feeder unit, preweaning mortality increased twofold, and subsequently, the number of pigs weaned per litter decreased by 19% (P < 0.005) during the 5-week epizootic. Also, the number of pigs born alive decreased by 6% during the epizootic (P < 0.05). No significant differences in production were observed between the 6-month periods before and after the epizootic. Actual cash flow analysis for the farm under isomarket conditions revealed a decreased net return of $2.40/inventoried sow/week, which was attributed to the disease during the epizootic, and a $0.46 decrease in net return/inventoried sow/week in the postepizootic period. Most seropositive herds have clinical signs less severe than those described in this herd, and the cost of eradication of the virus from a swine herd can be in excess of $200/inventoried sow. Thus, we believe that sufficient financial incentives are not available to all swine producers to ensure their enthusiastic cooperation in the effort to eradicate pseudorabies from the US swine population.