bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution of vitamin A‐storing lipid droplets in hepatic stellate cells in liver lobules—A comparative study
Authors: Higashi Nobuyo , Senoo Haruki 
Published: January 2003

AbstractTo investigate the storage mechanisms of vitamin A, we examined the liver of adult polar bears and arctic foxes, which physiologically store a large amount of vitamin A, by high‐performance liquid chromatography (HPLC), transmission electron microscopy (TEM) morphometry, gold chloride staining, fluorescence microscopy for the detection of autofluorescence of vitamin A, staining with hematoxylin‐eosin (H&E), Masson's trichrome, and Ishii and Ishii's silver impregnation. HPLC revealed that the polar bears and arctic foxes contained 1.8–1.9 × 104 nmol total retinol (retinol plus retinyl esters) per gram liver. In the arctic foxes, the composition of the retinyl esters was found to be 51.1% palmitate, 26.6% oleate, 15.4% stearate, and 7% linoleate. The hepatic stellate cells of the arctic animals were demonstrated by TEM to contain the bulk of the vitamin A‐lipid droplets in their cytoplasm. The liver lobules of the arctic animals showed a zonal gradient in the storage of vitamin A. The gradient was expressed as a symmetric crescendo‐decrescendo profile starting at the periportal zone, peaking at the middle zone, and sloping down toward the central zone in the liver lobule. The density (i.e., cell number per area) of hepatic stellate cells was essentially the same among the zones. The gradient and the composition of the retinyl esters in storing vitamin A were not changed by differences in the vitamin A amount in the livers. These results indicate that the heterogeneity of vitamin A‐storage capacity in hepatic stellate cells of arctic foxes and polar bears is genetically determined. Anat Rec Part A 271A:240–248, 2003. © 2003 Wiley‐Liss, Inc.
Impact of intracoronary radiation on in‐stent restenosis involving ostial lesions
Authors: Ajani Andrew E. , Waksman Ron , Cheneau Edouard , Leborgne Laurent , Pinnow Ellen , Canos Daniel A. , Satler Lowell F. , Pichard Augusto D. , Kent Kenneth M. , Lindsay Joseph 
Published: January 2003

AbstractThe aim of this study was to compare 6‐month clinical outcomes of patients with in‐stent restenosis (ISR) involving the ostium treated with intracoronary radiation therapy (IRT) compared to placebo therapy, and also to nonostial lesions treated with IRT. Coronary interventions in ostial lesions have a high rate of recurrence of restenosis. The impact of IRT on ostial ISR has been inadequately characterized. We assessed patients enrolled in gamma (192‐iridium) and beta (90‐yttrium, 32‐phosphorus) radiation trials for ISR at the Washington Hospital Center. Of patients receiving IRT, 105 (8%) patients had ostial ISR and 1,289 (92%) patients had nonostial ISR. Twenty‐seven patients had ostial ISR and received placebo therapy. Baseline demographic and angiographic and procedural details were similar, except ostial IRT patients had a trend toward shorter lesions (15.4 ± 10.8 vs. 24.1 ± 12.2 mm; P < 0.001) and had a higher rate of saphenous vein graft disease (46% vs. 19%; P < 0.001) compared to nonostial IRT patients. At 6 months, ostial lesions treated with IRT for ISR had a reduced rate of target lesion revascularization (TLR) compared to ostial lesions treated with placebo (15% vs. 43%; P = 0.004). Outcomes at 6 months were similar for the ostial and nonostial IRT groups including TLR (15% vs. 14%; P = 0.80) and composite major adverse cardiac events (18% vs. 15%; P = 0.46). Intracoronary radiation therapy is effective for ostial in‐stent restenotic lesions and should be comfortably used for this challenging anatomic location. Cathet Cardiovasc Intervent 2003;58:175–180. © 2003 Wiley‐Liss, Inc.
Intraluminal blunt microdissection for angioplasty of coronary chronic total occlusions
Authors: Whitbourn Robert J. , Cincotta Marion , Mossop Peter , Selmon Matthew 
Published: January 2003

AbstractThis study describes a new approach to crossing coronary chronic total occlusions using controlled blunt microdissection and its successful application to coronary angioplasty in three patients. After guidewire techniques failed to cross the occlusions, the blunt intraluminal microdissection catheter was deployed. Actuation of a hinged jaw on the catheter distal assembly created a channel for the guidewire through the diseased segment, in the true lumen (a right coronary and a left circumflex artery) and subintimally (a circumflex artery), to allow angioplasty and stenting. Coronary circulation improved from TIMI grade 0 to 3. Angina was relieved in all three cases. Subsequent angiography for two cases, 2 and 19 months after PTCA, respectively, showed restored flow and patent stented regions. Cathet Cardiovasc Intervent 2003;58:194–198. © 2003 Wiley‐Liss, Inc.
Catheter‐based coronary bypass: A development update
Authors: Oesterle Stephen N. , Reifart Nicolaus , Hayase Motoya , Hauptmann Eugen , Low Reginald , Erbel Raimund , Haude Michael , Dirsch Olaf , Schuler Gerhard C. , Virmani Renu , Yeung Alan C. 
Published: January 2003

AbstractCatheter‐based coronary bypass has evolved since its origin in 1995. We present a status update of one version of catheter‐based bypass, percutaneous in situ coronary venous arterialization (PICVA), its successes and failures, and the many questions and challenges that remain. Initial clinical experience with PICVA demonstrated promising mitigation of angina in no‐option patients, but was complicated by a relatively low procedural completion rate and a high incidence of MACE. A great deal was learned in these initial cases. The system of devices is currently undergoing significant modification, and further clinical study is underway. Cathet Cardiovasc Intervent 2003;58:212–218. © 2003 Wiley‐Liss, Inc
Initial human experience with the Amplatzer perimembranous ventricular septal occluder device
Authors: Bass John L. , Kalra G.S. , Arora Ramesh , Masura Jozef , Gavora Pavlo , Thanopoulos Basil D. , Torres William , Sievert Horst , Carminati Mario , Fischer Gunther , Ewert Peter 
Published: January 2003

AbstractTranscatheter closure of perimembranous ventricular septal defects with coils or devices designed to close other lesions may be complicated by embolization or aortic insufficiency. A new asymmetric Amplatzer perimembranous ventricular septal occluder and delivery system was specifically designed for perimembranous defects. This report describes the first use of this device in 27 patients. Implantation was successful in 25 (93%), with 1 removed for device‐related aortic insufficiency and inability to position the delivery sheath in another. Device orientation was excellent when the device was initially advanced through a standard delivery sheath positioned in the left ventricular apex. Twenty‐three had complete occlusion within 1 week (92%), with a tiny (< 2 mm) residual shunt in the other two. In the 25 subjects with the device left in place, device‐related aortic or tricuspid insufficiency, arrhythmias, and embolization were not observed. These excellent acute results need to be confirmed by long‐term follow‐up. Cathet Cardiovasc Intervent 2003;58:238–245. © 2003 Wiley‐Liss, Inc.
Revascularization of a thrombosed aortopulmonary shunt with the use of the AngioJet thrombectomy system
Authors: Donnelly Jon P. , Ryan Thomas J. 
Published: January 2003

AbstractWe report the use of the AngioJet F140 rheolytic catheter to recannalize an acutely thrombosed aortopulmonary shunt in a 21‐year‐old female with palliated, complex congenital heart disease. After extracting the thrombus that filled the entire length of the shunt, three stents were placed at sites where the lumen was compromised by distortion or thrombus. Unobstructed flow was restored to the left pulmonary artery that persisted at 1‐year follow‐up. Cathet Cardiovasc Intervent 2003;58:268–271. © 2003 Wiley‐Liss, Inc.
Simultaneous bilateral carotid stenting for postendarterectomy restenosis
Authors: Lesley Walter S. , Lazo Al , Kazmierczak Chris D. , Wilseck Jeffrey M. 
Published: January 2003

AbstractWe report the successful treatment of bilateral postendarterectomy restenosis using simultaneously placed, bilateral carotid SMART stents and balloon angioplasty. Technical aspects and the results of 29‐month follow‐up are presented. The benefits derived from single‐setting bilateral carotid stenting versus staged bilateral stenting are discussed. Cathet Cardiovasc Intervent 2003;58:147–150. © 2003 Wiley‐Liss, Inc.
Alcohol septal ablation complicated by complete heart block and permanent pacemaker failure
Authors: Valettas Nicholas , Rho Robert , Beshai John , Lloyd Caroline T. , Ross Heather M. , Kocovic Dusan , Herrmann Howard C. 
Published: January 2003

AbstractAlcohol septal ablation is a novel catheter‐based technique for the treatment of obstructive hypertrophic cardiomyopathy. Complete heart block complicates the procedure in 7%–30% of cases and necessitates the prophylactic insertion of a temporary pacing wire in all patients who do not have a permanent pacemaker. We describe a case of alcohol septal ablation complicated by complete heart block and failure to capture by both a permanent pacemaker and an implantable cardioverter defibrillator (ICFD) with pacing capabilities. Cathet Cardiovasc Intervent 2003;58:189–193. © 2003 Wiley‐Liss, Inc.
Brachytherapy for renal artery in‐stent restenosis
Authors: Aslam Muhammad Shakil , Balasubramanian Jayalakshmi , Greenspahn Bruce R. 
Published: January 2003

AbstractWe describe a 66‐year‐old female who presented with recurrent acute pulmonary edema and uncontrolled hypertension. She was diagnosed with left renal artery stenosis and treated with angioplasty and stent placement. Her clinical status improved initially but symptoms recurred within 4 months. Further evaluation documented renal artery in‐stent restenosis, which was successfully treated with cutting balloon angioplasty followed by brachytherapy. The feasibility of renal artery brachytherapy and short‐term follow‐up is presented. Cathet Cardiovasc Intervent 2003;58:151–154. © 2003 Wiley‐Liss, Inc.
Fracture of cutting balloon microsurgical blade inside coronary artery during angioplasty of tough restenotic lesion: A case report
Authors: Haridas K.K. , Vijayakumar M. , Viveka Kumar , Rajesh T. , Mahesh N.K. 
Published: January 2003

AbstractWe report a rare complication due to fracture of the blade of a cutting balloon in the setting of a tough nondilatable restenotic lesion. This resulted in a mural hematoma as a result of dissection of coronary artery. Cathet Cardiovasc Intervent 2003;58:199–201. © 2003 Wiley‐Liss, Inc.
Widespread regional myocardial transfection by plasmid encoding Del‐1 following retrograde coronary venous delivery
Authors: Hou Dongming , Maclaughlin Fiona , Thiesse Mary , Panchal Vipul R. , Bekkers Bas C.A.M. , Wilson Elizabeth A. , Rogers Pamela I. , Coleman Michael C. , March Keith L. 
Published: January 2003

AbstractThis study quantifies myocardial transfection following percutaneous retrograde coronary venous delivery (RCVD) of a plasmid encoding human Del‐1. RCVD of Del‐1, GFP plasmid, or marker dye was conducted in 14 pigs. After selective cannulation of a coronary vein, a delivery site was confirmed by contrast injection and myocardial blush. Ten milliliters of plasmid hDel‐1 or GFP was administered. Animals were euthanized 3 and 7 days post‐RCVD. hDel‐1 gene expression was evaluated by quantitative RT‐PCR. An average myocardial expression of 4.5 × 105 copies hDel‐1/μg total RNA was observed within the approximately 5 × 5 cm2 target tissue of the left ventricle. GFP expression was detected by fluorescent microscopy. hDel‐1 protein expression was confirmed by immunohistochemistry. Regionalized myocardial expression was found in all pigs. hDel‐1 RNA was not found in distant tissues except in the three pigs with prominent venovenous washout (PVW). These levels were 3 to 4 log unites lower than those found in myocardium. Single retrograde coronary venous administration resulted in efficient regional myocyte transfection of hDel‐1 and GFP. This method may be useful and clinically feasible for myocardial angiogenesis therapy. Cathet Cardiovasc Intervent 2003;58:207–211. © 2003 Wiley‐Liss, Inc.
Lesion length assessment using a new vessel caliper
Authors: Wolyn Robert , Dixon Simon R. , Almany Steven L. , Korotko Joe , Huang Raywin R. , O'Neill William W. 
Published: January 2003

AbstractAssessment of coronary lesion length using visual estimation or quantitative coronary angiography can be unreliable. We tested the accuracy of a new handheld caliper device to measure lesion length in a bench‐top model and in eight patients undergoing percutaneous coronary intervention (PCI). Caliper–derived length measurements were compared to the known reference distance in the bench‐top model and visual or intravascular ultrasound (IVUS)‐derived measurements in vivo. In the coronary model, caliper‐derived measurements were accurate and correlated well with known reference distances regardless of the angiographic projection. During PCI, there was a poor correlation between the best visual estimate of length and IVUS‐derived measurements. In contrast, caliper‐derived measurements correlated closely with IVUS‐derived measurements. This handheld caliper provides a simple and accurate method of assessing intracoronary lesion length and may be particularly useful during coronary stenting and when adjunctive brachytherapy is performed. Cathet Cardiovasc Intervent 2003;58:168–174. © 2003 Wiley‐Liss, Inc.
Primary angioplasty of an anomalous left main coronary artery: Diagnostic and technical considerations
Authors: Lanzieri Mark , Khabbaz Kamal , Salomon Robert N. , Kimmelstiel Carey 
Published: January 2003

AbstractIn this case of primary angioplasty of an anomalous left main coronary artery, a method of identifying the course of these vessels is reviewed. Unique electrocardiographic findings relative to left main occlusion, aiding in suspecting this anomaly before angiographic study, are presented and the technical approach to revascularization is discussed. Cathet Cardiovasc Intervent 2003;58:185–188. © 2003 Wiley‐Liss, Inc.
Congenital heart disease in adults: Catheterization laboratory considerations
Authors: Fox James M. , Bjornsen Katherine D. , Mahoney Larry T. , Fagan Thomas E. , Skorton David J. 
Published: January 2003

AbstractCongenital heart defects are the most common birth defects and represent an increasing proportion of adolescent and adult patients followed by cardiologists. While many of these patients have undergone successful palliative or corrective surgery with excellent functional results, most of them still require careful follow‐up. Further, even complex lesions may first be diagnosed in adolescence and adulthood. Therefore, cardiologists caring for adults need to become more familiar with these defects. Assessment of the patient with known or suspected congenital heart defects requires a careful history, physical examination, and noninvasive assessment. In addition, the catheterization laboratory remains a critical venue for diagnosis and, increasingly, therapy. Pressure measurements, oximetry, and angiography remain cornerstones of diagnosis in selected patients and a variety of interventional procedures have become viable therapeutic alternatives in both pre‐ and postoperative patients. Cathet Cartiovasc Intervent 2003;58:219–231. © 2003 Wiley‐Liss, Inc.
Calculation of damping torque of power systems compensated with TCSC
Authors: Kakimoto Naoto , Phongphanphanee Anan 
Published: January 2003

AbstractThe thyristor‐controlled series capacitor (TCSC) is being developed to improve the transmission capability of power systems. The TCSC is thought to compensate transmission line reactance without causing subsynchronous resonance (SSR). However, in order to evaluate its effect quantitatively, we must calculate the frequency response of the generator damping torque. Simulations need a long computing time, and it is hard to choose the frequency freely. In this paper, we propose a method of analytically calculating the damping torque. First, when a generator rotor oscillates at some frequency, two voltage components appear. We analytically calculate the damping torque from small current variations due to the voltage components. The damping characteristic changes depending on the method of firing thyristors. The best characteristic is obtained when triggering with reference to the fundamental wave of TCSC voltage or current is used. By choosing an appropriate firing angle, we can drastically reduce negative damping by the TCSC. The damping characteristic is closely related to the system impedance. The fact that the TCSC has large resistance in the 0 to 60 Hz range helps significantly in improving the characteristic. Lastly, numerical simulations of SSR are used to examine the validity of our investigation. © 2003 Wiley Periodicals, Inc. Electr Eng Jpn, 143(1): 39–49, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.10068
Performance analysis of closed loop in a CCMHD single power generation system
Authors: Yamasaki Hiroyuki , Uehara Luis Vila , Okuno Yoshihiro 
Published: January 2003

AbstractResults of numerical study on performance of closed loop in the CCMHD single power generation system are presented. The small closed loop with thermal input of 2.3 MW was designed. The fluid‐dynamical and thermal performance of the closed loop was calculated for the first time by one‐dimensional numerical simulation. The result indicates that pressure loss and heat loss are large in the designed small closed loop. The large pressure loss was due to the wall friction or the pebble bed and it occurred at the supersonic nozzle, the MHD channel, and the regenerative heat exchanger used for argon cooling. The enthalpy extraction remained low (= 17.6%) owing to the large pressure loss and as a result, the thermal efficiency of the closed loop remained only 21.6%. The enthalpy extraction and the thermal efficiency became maximal at the optimal inlet total pressure of 0.60 MPa. At inlet total pressure lower than the optimal one, the electric conductivity, the flow velocity, and the electric efficiency became higher. However, the total pressure ratio (= exit/inlet) in the MHD channel became larger particularly at low inlet total pressure because of the increase in the pressure loss due to pebble bed and wall friction. These led to the existence of the optimal inlet total pressure. © 2003 Wiley Periodicals, Inc. Electr Eng Jpn, 143(1): 27–38, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.10118
Development and characterization of CHO repair‐proficient cell lines for comparative mutagenicity and metabolism of heterocyclic amines from cooked food
Authors: Wu Rebekah W. , Panteleakos Frances N. , Felton James S. 
Published: January 2003

AbstractIn order to understand the role of repair and metabolism in the mutagenicity of heterocyclic amines from cooked foods, we previously developed the nucleotide excision repair‐deficient CHO 5P3NAT2 cell line engineered to coexpress the mouse CYP1A2 and human N‐acetyltransferase genes. In the present study, we have made a matched repair‐competent cell line by mutagenizing 5P3NAT2 cells with ethyl methanesulfonate and selecting for resistance to cytotoxicity by 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ). In the differential cytotoxicity (DC) assay, 4 out of 15 clones showed no cytotoxic effect with IQ at the highest dose (30 μg/ml) tested, in contrast to repair‐deficient 5P3NAT2 cells, which showed ∼ 100% cytotoxicity at 0.3 μg/ml. Subsequently, these IQ‐resistant clones were examined for resistance to killing by UV irradiation. All four IQ‐resistant clones, which show resistance to UV similar to that of repair‐proficient AA8 cells, still express both the CYP1A2 and N‐acetyltransferase genes. Sequence analysis of CXPD cDNA from the 5P3NAT2R9 clone revealed an A:T→G:C reversion event at the site of the UV5 mutation. This base change results in reversion of the codon 116 tyrosine in UV5 cells back to the original cysteine in AA8 cells, thereby restoring wild‐type CXPD activity and repair function. In contrast to 5P3NAT2 cells, the repair‐proficient 5P3NAT2R9 revertant cell line shows little IQ‐induced cell killing, and dramatically lower levels of induced mutation at the adenine phosphoribosyltransferase (Aprt) gene locus over the range of 2–40 μg/ml IQ. This matched pair of repair‐proficient/deficient cell lines can provide insight not only into the genotoxicity of heterocyclic amine dietary carcinogens such as IQ and PhIP, but also into the effects of nucleotide excision repair on the ultimate mutagenicity of these compounds. Environ. Mol. Mutagen. 41:7–13, 2003. © 2003 Wiley‐Liss, Inc.
Using CTLL‐2 and CTLL‐2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test
Authors: Meintières Sophie , Biola Armelle , Pallardy Marc , Marzin Daniel 
Published: January 2003

AbstractIn vitro assays for chromosome aberrations (i.e., in vitro micronucleus and in vitro metaphase analysis tests) frequently produce false‐positive or exaggerated‐positive results. Our previous work suggested that apoptosis interferes with these tests, producing misleading results. These previous studies were conducted by performing the in vitro micronucleus test in CTLL‐2 cells and a CTLL‐2 cell derivative stably transfected with the apoptosis inhibitor gene bcl2. In the present study, these previous observations were extended by examining micronucleus induction with a larger number of compounds in both CTLL‐2 and CTLL‐2 bcl2 cells and measuring apoptosis with annexin V‐FITC. Both cell lines were treated with different classes of compounds that were anticipated to be exclusively apoptosis inducers, or compounds known to be clastogens or aneugens, some of which were anticipated to be both genotoxic and apoptotic. We were able to confirm that compounds that are only apoptogenic induced micronuclei in CTLL‐2 but not CTLL‐2 bcl2 cells, indicating that the positive responses are due to apoptosis in CTLL‐2 cells. Some genotoxins (clastogens and aneugens) did not produce apoptosis by the annexin V assay and gave similar responses in CTLL‐2 and CTLL‐2 bcl2 cells. Finally, higher responses were induced in CTLL‐2 cells compared to CTLL‐2 bcl2 cells that were treated with aneugens or clastogens that were also apoptosis inducers, suggesting that the greater response in CTLL‐2 cells is a consequence of both genotoxicity and apoptosis. Finally, it was demonstrated that just eliminating CTLL‐2 cells having three or more micronuclei from scoring was not adequate for correctly evaluating agents that only produce apoptosis. The results indicate that coupling the in vitro micronucleus test in both CTLL‐2 and CTLL‐2 bcl2 cells with the measurement of apoptosis is able to distinguish the genotoxic effects of a test compound from its apoptotic potential and is able to avoid interference from apoptosis in the in vitro micronucleus test. These observations may provide the basis for a useful genotoxicity assay. Environ. Mol. Mutagen. 41:14–27, 2003. © 2003 Wiley‐Liss, Inc.