bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of potential immunologic resilience in the healing process of diabetic foot ulcers
Authors: Cheng Yifeng , Ren Lei , Niyazi Aihemaitijiang , Sheng Li , Zhao Yang 
Published: November 2023

AbstractDiabetic foot ulcers (DFUs) are one of the most common and challenging complications of diabetes, yet our understanding of their pathogenesis remains limited. We collected gene expression data of DFU patients from public databases. Bioinformatics tools were applied for systematic analysis, including the identification of differentially expressed genes (DEGs), weighted gene co‐expression network analysis (WGCNA) and enrichment analysis. We further used single‐cell RNA sequencing to identify the distribution of different cell populations in DFU. Finally, key results were validated using reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and flow cytometry. We identified 217 DEGs between ulcerated and healthy skin, and 37 DEGs between healing ulcers and ulcers. WGCNA revealed that the cyan module had the highest positive correlation with healthy skin and negative correlation with ulcers. The black module had the highest negative correlation with healthy skin and positive correlation with ulcers. Enrichment analysis showed that the genes in the cyan module were mainly associated with complement and coagulation cascades, while the genes in the black module were mainly associated with the IL‐17 signalling pathway. In addition, CD8 T cells were significantly lower in ulcers than in healthy and healing ulcers. By comparing marker genes of CD8 T cells, we identified key genes in the cyan and black modules and validated their expression using RT‐qPCR. The proportion of CD8 T cells was increased in healing ulcers. Flow cytometry detected increased levels of CD8 T, B and natural killer cells in healing ulcers. CD8 T cells and related key genes play an important role in the healing process of DFU. The results of this study provide a new perspective for understanding the pathogenesis and treatment of DFU.
What helps or hinders nurses to lead funded research projects? A survey of UK nurse lead‐investigators
Authors: Farquharson Barbara 
Published: November 2023

AbstractBackgroundThere have been recurring UK initiatives to increase nurse research capability but little robust evaluation of long‐term effectiveness. More nurses undertake doctorates, yet few lead major funded projects. Previous research suggests potential explanations but the perspectives of nurse lead‐investigators themselves have not been examined.AimTo explore the perceptions of nurse lead‐investigators about what has helped or hindered them to lead funded research projects.MethodsLead investigators of research projects from major UK funders (1 Apr 2017–Sept 2022) were identified from publicly available data. University profiles were screened to identify registered nurses. Entire population was approached (no sample size calculation required). Consenting participants completed an online survey (five open questions).ResultsA total of 65 nurse‐lead investigators were identified, 36 (55%) completed the survey (20 December 2022 to 17 February 2023). Participants identified Building (multi‐disciplinary) collaborations and mentorship as having been most important to their success. High‐quality mentoring was also identified as most important in helping novice nurse researchers become leaders. Participants highlighted the critical importance of being supported by individuals with a track record of funding success and benefits of being situated in research‐supportive environments. Lack of career pathway/infrastructure and being unable to pursue research due to competing clinical/teaching priorities were identified as most unhelpful to this group AND the most common reasons for peers not going on to lead research.ConclusionsEnsuring access to mentors with an established track record is an important component of schemes to increase research capability in nurses. Funded, protected time for research and career structures that reward the significant skill development required to succeed in a competitive, multi‐disciplinary funding arena is important.ImpactInterdisciplinary collaboration and mentorship by experienced researchers are critical to success and should be incorporated into future interventions to increase research capability in nurses. No patient or public contribution (as exploring a professional issue).
Impact of intracardiac echocardiography versus transesophageal echocardiography guidance on left atrial appendage occlusion procedures: A meta‐analysis
Authors: Diaz Juan Carlos , Bastidas Oriana , Duque Mauricio , Marín Jorge E. , Aristizabal Julian , Niño Cesar D. , Hoyos Carolina , Matos Carlos D. , Gabr Mohamed , Steiger Nathaniel A. , Kapur Sunil , Sauer William H. , Romero Jorge E. 
Published: November 2023

AbstractBackgroundIntracardiac echocardiography (ICE) is increasingly used during left atrial appendage occlusion (LAAO) as an alternative to transesophageal echocardiography (TEE). The objective of this study is to evaluate the impact of ICE versus TEE guidance during LAAO on procedural characteristics and acute outcomes, as well the presence of peri‐device leaks and residual septal defects during follow‐up.MethodsAll studies comparing ICE‐guided versus TEE‐guided LAAO were identified. The primary outcomes were procedural efficacy and occurrence of procedure‐related complications. Secondary outcomes included lab efficiency (defined as a reduction in in‐room time), procedural time, fluoroscopy time, and presence of peri‐device leaks and residual interatrial septal defects (IASD) during follow‐up.ResultsTwelve studies (n = 5637) were included. There were no differences in procedural success (98.3% vs. 97.8%; OR 0.73, 95% CI 0.42–1.27, p = .27; I2 = 0%) or adverse events (4.5% vs. 4.4%; OR 0.81 95% CI 0.56–1.16, p = .25; I2 = 0%) between the ICE‐guided and TEE‐guided groups. ICE guidance reduced in in‐room time (mean‐weighted 28.6‐min reduction in in‐room time) without differences in procedural time or fluoroscopy time. There were no differences in peri‐device leak (OR 0.93, 95% CI 0.68–1.27, p = 0.64); however, an increased prevalence of residual IASD was observed with ICE‐guided versus TEE‐guided LAAO (46.3% vs. 34.2%; OR 2.23, 95% CI 1.05–4.75, p = 0.04).ConclusionICE guidance is associated with similar procedural efficacy and safety, but could result in improved lab efficiency (as established by a significant reduction in in‐room time). No differences in the rate of periprocedural leaks were found. A higher prevalence of residual interatrial septal defects was observed with ICE guidance.
The impact of early cryoballoon ablation on clinical outcome in patients with atrial fibrillation: From the Korean cryoballoon ablation registry
Authors: Kwon Chang Hee , Choi Ji‐Hoon , Oh Il‐Young , Lee So‐Ryoung , Kim Ju Youn , Lee Sung Ho , Park Junbeom , Kim Ki‐Hun , Yang Pil‐Sung , Kim Jun‐Hyung , Shim Jaemin , Cha Myung‐Jin , Lim Hong Euy 
Published: November 2023

AbstractIntroductionInfluence of early atrial fibrillation (AF) ablation, particularly cryoballoon ablation (CBA), on clinical outcome during long‐term follow‐up has not been clarified. The objective was to determine whether an early CBA (diagnosis‐to‐ablation of ≤6 months) strategy could affect freedom from AF recurrence after index CBA.MethodsThe study included 2605 patients from Korean CBA registry data with follow‐up >12 months after de novo CBA. The primary outcome was recurrence of atrial tachyarrhythmias (ATs) of ≥30‐s after a 3‐month blanking period.ResultsCompared to patients in early CBA group, patients in late CBA group had higher prevalence of diabetes, congestive heart failure, and chronic kidney disease, and higher mean CHA2DS2‐VAS score. During mean follow‐up of >21 months, ATs recurrence was detected in 839 (32.2%) patients. The early CBA group showed a significantly lower 2‐year recurrence rate of ATs than the late CBA group (26.1% vs. 31.7%, p = 0.043). In subgroup analysis, the early CBA group showed significantly higher 1‐year and 2‐year freedom from ATs recurrence than the late CBA group only in paroxysmal atrial fibrillation (PAF) patients in overall and propensity score matched cohorts. Multivariate analysis showed that early CBA was an independent factor for preventing ATs recurrence in PAF (hazard ratio: 0.637; 95% confidence intervals: 0.412–0.984).ConclusionEarly CBA strategy, resulting in significantly lower ATs recurrence during 2‐year follow‐up after index CBA, might be considered as an initial rhythm control therapy in patients with paroxysmal AF.
PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1
Authors: Wang Ya‐Wen , Zhu Wen‐Jie , Ma Ran‐Ran , Tian Ya‐Ru , Chen Xu , Gao Peng 
Published: November 2023

AbstractLong noncoding RNAs (lncRNAs) play critical roles in the carcinogenesis and progression of cancers. However, the role and mechanism of the pseudogene lncRNA PIN1P1 in gastric carcinoma remain unclear. The expression and effects of lncRNA PIN1P1 in gastric cancer were investigated. The transcriptional regulation of CREB1 on PIN1P1 was determined by ChIP and luciferase assays. The mechanistic model of PIN1P1 in gastric cancer was further explored by RNA pull‐down, RIP and western blot analysis. PIN1P1 was overexpressed in gastric cancer tissues, and upregulated PIN1P1 predicted poor prognosis in patients. CREB1 was directly combined with the promoter region of PIN1P1 to promote the transcription of PIN1P1. CREB1‐mediated enhanced proliferation, migration and invasion could be partially reversed by downregulation of PIN1P1. Overexpressed PIN1P1 promoted the proliferation, migration and invasion of gastric cancer cells, whereas decreased PIN1P1 showed the opposite effects. PIN1P1 directly interacted with YBX1 and promoted YBX1 protein expression, leading to upregulation of PIN1, in which E2F1 may be involved. Silencing of YBX1 during PIN1P1 overexpression could partially rescue PIN1 upregulation. PIN1, the parental gene of PIN1P1, was elevated in gastric cancer tissues, and its upregulation was correlated with poor patient outcomes. PIN1 facilitated gastric cancer cell proliferation, migration and invasion. To sum up, CREB1‐activated PIN1P1 could promote gastric cancer progression through YBX1 and upregulating PIN1, suggesting that it is a potential target for gastric cancer.
Vicarious trauma in nursing: A hybrid concept analysis
Authors: Wu Yanming , Bo Enhui , Yang Erming , Mao Ya , Wang Qiaohong , Cao Huili , He Xingyue , Yang Hui , Li Yang 
Published: November 2023

AbstractBackgroundVicarious trauma can significantly affect the physical and mental health of nurses, as well as their ability to provide quality of care. However, the concept of vicarious trauma has received limited attention and remains controversial in the nursing context.ObjectiveThe purpose of this study was to clarify and define the concept of vicarious trauma as it pertains to the nursing context.MethodsThe Schwartz and Kim's three‐stage hybrid concept analysis method was used to define the concept. In the theoretical phase, PubMed, CINAHL, OVID, Medline, Embase, Web of Science, Scopus, ProQuest, PsycINFO, CNKI database, VIP database and Wanfang database were used using keywords “nurs*”and “vicarious trauma*”, resulting in a total of 25 papers. In the fieldwork phase, we conducted participatory observations in three hospitals and semi‐structured in‐depth interviews with 18 clinical nurses from seven cities. In the analysis phase, the results of the previous two phases were integrated to develop a comprehensive concept of vicarious trauma in nursing.ResultsBased on the results of the theoretical and field phases, we propose the concept of vicarious trauma in nursing as follows: vicarious trauma is a psychological trauma impacting nurses' cognitive schema which they may experience in clinical settings or on social media, resulting from deeply empathize with the physical or emotional trauma of patients, family, or colleagues, such as patients' physical injuries or death, family's grieving feelings and colleagues' received threats and attacks. Positively, vicarious trauma can transform into vicarious post‐trauma growth through repositioning and connection, nourishing nurses and promoting their personal and professional development.ConclusionThe concept of vicarious trauma in nursing is multidimensional and holistic. This study clarifies the concept of vicarious trauma in nursing using the hybrid concept analysis, providing a framework for future research and practice on vicarious trauma in the nursing field.Patient or Public ContributionNurses contributed to the conduct of the study by participating in the data collection via interviews.
Masculine God Imagery and Sense of Life Purpose: Examining Contingencies with America's “Four Gods”
Authors: Upenieks Laura , Bonhag Rebecca 
Published: November 2023

AbstractThough God imagery has been extensively studied within sociological and psychological traditions, much less attention has been paid to gendered God concepts and their connections to well‐being. Previous work has suggested that God images may reflect ontological assumptions that inform interpretations of the world as well as one's place within it. We argue that the relationship between masculine God imagery and a sense of life purpose may vary by gender and depend on further contingencies of other God images held by individuals’ images of God as engaged, angry, critical, and distant. Drawing on nationally representative data from the 2007 Baylor Religion Survey (n= 415 men andn= 577 women), our results suggest that stronger masculine God imagery was associated with greater life purpose for men, and lower life purpose for women. We also observed that stronger beliefs in an engaged God appeared to weaken the association between masculine God imagery and lower life purpose for women, while men who did not support masculine gender ideology reported lower life purpose if they endorsed stronger beliefs in an angry or critical God. We interpret our results by drawing on research at the intersection of gender, religion, and theology, and suggest several directions for future work.
In God We Distrust: Christian Nationalism and Anti‐Atheist Attitude
Authors: NIE FANHAO 
Published: November 2023

AbstractPrior research found that Christian nationalism, a belief that integrates Christian identity with American national identity, was associated with more negative views toward marginalized groups, such as religious minorities. Relatively less known is the relationship between Christian nationalism and attitudes toward atheists. Specifically, even less is known about whether or not Christian nationalism that is above individuals at the macro, geographic level may influence individual views of atheists. Using nationally representative multilevel data, this study found that higher individual Christian nationalism was associated with more negative views toward atheists. No robust state‐level Christian nationalism influence was found for anti‐atheist attitude. However, Blacks, relative to Whites, were less likely to view atheists negatively in states where Christian nationalism was higher. Meanwhile, in states where Christian nationalism was higher, Black Protestants were less prejudiced against atheists compared to Evangelical Protestants.
Skull morphology of bottlenose dolphins worldwide and patterns of adaptation between coastal and offshore environments
Authors: Oxford‐Smith N. , Ruta M. , Gao A. , Viaud‐Martinez K. A. , Sabin R. , Herman J. , Ososky J. , Tajima Y. , Yamada T. K. , Kaliontzopoulou A. , Moura A. E. 
Published: November 2023

AbstractThe morphological differentiation between coastal and offshore ecotypes of bottlenose dolphins (genus Tursiops) has been researched since the 1960s, particularly in T. truncatus (Montagu, 1821), although most morphological studies have focused on localized populations. Therefore, it is unclear how patterns observed in these individual populations integrate within the global morphological variation. Here we carry out a meta‐analysis of global morphological variation between coastal and offshore ecotypes from 532 museum specimens, using both linear measurements (LM; 282 specimens), and shape data using geometric morphometrics (GM; 290 specimens). Together these analyses show consistent differentiation in skull morphology between coastal and offshore ecotypes, and provide a detailed description of variation patterns within each ecotype. These patterns show high individual morphological variation in the coastal ecotype between locations, while the offshore ecotype appears morphologically more uniform across the areas sampled. Overall, most skull shape variation can be described by features noticeable dorsally in the structures of the rostrum, whereas more limited change was found in ventral traits. Our results suggest that individual coastal populations may vary according to local environmental conditions, while those corresponding to the offshore ecotype appear to share similar morphological characteristics that might increase fitness in offshore habitats.
Antimicrobial peptides in treatment of Stage III Grade B periodontitis: A randomized clinical trial
Authors: Xiang Shuchang , Han Nannan , Xie Yongmei , Du Juan , Luo Zhenhua , Xu Junji , Liu Yi 
Published: November 2023

AbstractBackgroundTo evaluate the effects of antimicrobial peptides (AMPs) on Stage III Grade B periodontitis.MethodsThis trial abided by the principle of consistency test, approved by ethics committee and registered in clinical trials. All qualified 51 patients with Stage III Grade B periodontitis were randomly divided into three groups: SRP group, SRP with minocycline hydrochloride (Mino group) as Control groups, and SRP with AMPs (AMP group) as the Test group. Clinical examinations and subgingival plaques were monitored at baseline and at 7 and 90 days after treatment in the SRP, SRP with AMP and Mino groups.ResultsThe AMP group (Test group) had a reduced PD (Periodontal probing depth) and an attachment gain significantly higher than SRP and Mino groups (Control groups) at day 90. The abundance of periodontal pathogens was decreased in the AMP group at 7 and 90 days compared with the SRP group and Mino group. Only the AMP group showed an increase the abundance of periodontal probiotics including Capnocytophaga, Gemella, and Lactobacillus at 7 and 90 days.ConclusionsThis study shows that AMPs as an adjunct to SRP promote additional clinical and microbiological benefits in the treatment of Stage III Grade B periodontitis.
Electrical stimulation prevents condyle and subchondral degeneration following the masseter atrophy
Authors: Wu Chuan , Zhang Qunyan , Zheng Xiuyun , Han Quancheng , Fu Chunfeng , Liu Xiaoyu , Wu Tingting 
Published: November 2023

AbstractObjectiveThere is a strong relationship between masticatory muscle atrophy and condyle degeneration. Although electrical stimulation (ES) is an effective treatment for muscle atrophy, its influence on the underlying condyle is unclear. This study aimed to investigate whether ES can prevent condyle degradation during the stage of masseter muscle atrophy.Materials and MethodsSix‐week‐old rats were randomly divided into the control, botulinum toxin (BTX), or BTX + ES group. BTX was injected into the bilateral masseters of rats to induce masseter atrophy. The left‐side masseters without ES treatment were served as BTX group, and the right‐side masseters received ES with different parameters (5 mA/10 Hz, 5 mA/50 Hz, 6 mA/10 Hz, 6 mA/50 Hz, 7 mA/10 Hz, and 7 mA/50 Hz) were served as BTX + ES groups. After 4 weeks, micro‐CT and qualitative or quantitative analysis of osteogenesis, chondrogenesis, and angiogenesis‐related genes in condyles were conducted.ResultsES, especially at 7 mA/50 Hz, significantly attenuated masseter atrophy, condyle degeneration, and subchondral bone loss. Moreover, the upregulation of related proteins, including collagen 1, osteocalcin, bone morphogenetic protein 2, collagen 2a, and vascular endothelial growth factor were observed.ConclusionES partly rescued condylar degeneration and subchondral bone loss following masseter atrophy.
FoxO1 knockdown inhibits RANKL‐induced osteoclastogenesis by blocking NLRP3 inflammasome activation
Authors: Wang Zhanqi , Luo Wenxin , Zhang Guorui , Li Haiyun , Zhou Feng , Wang Dongyang , Feng Xuan , Xiong Yi , Wu Yingying 
Published: November 2023

AbstractObjectivesThis study aimed to elucidate the connection between osteoclastic forkhead transcription factor O1 (FoxO1) and periodontitis and explore the underlying mechanism by which FoxO1 knockdown regulates osteoclast formation.Materials and MethodsA conventional ligature‐induced periodontitis model was constructed to reveal the alterations in the proportion of osteoclastic FoxO1 in periodontitis via immunofluorescence staining. Additionally, RNA sequencing (RNA‐seq) was performed to explore the underlying mechanisms of FoxO1 knockdown‐mediated osteoclastogenesis, followed by western blotting, quantitative polymerase chain reaction, and enzyme‐linked immunosorbent assay.ResultsFoxO1+ osteoclasts were enriched in the alveolar bone in experimental periodontitis. Moreover, FoxO1 knockdown led to impaired osteoclastogenesis with low expression of osteoclast differentiation‐related genes, accompanied by an insufficient osteoclast maturation phenotype. Mechanistically, RNA‐seq revealed that the nuclear factor kappa B (NF‐κB) and nucleotide‐binding oligomerization domain‐like receptor family pyrin domain containing 3 (NLRP3) inflammasome signaling pathways were inhibited in FoxO1‐knockdown osteoclasts. Consistent with this, MCC950, an effective inhibitor of the NLRP3 inflammasome, substantially attenuated osteoclast formation.ConclusionsFoxO1 knockdown contributed to the inhibition of osteoclastogenesis by effectively suppressing NF‐κB signaling and NLRP3 inflammasome activation. This prospective study reveals the role of FoxO1 in mediating osteoclastogenesis and provides a viable therapeutic target for periodontitis treatment.
Assessing the safety of new germicidalfar‐UVCtechnologies
Authors: Görlitz Maximilian , Justen Lennart , Rochette Patrick J. , Buonanno Manuela , Welch David , Kleiman Norman J. , Eadie Ewan , Kaidzu Sachiko , Bradshaw William J. , Javorsky Emilia , Cridland Nigel , Galor Anat , Guttmann Martin , Meinke Martina C. , Schleusener Johannes , Jensen Paul , Söderberg Per , Yamano Nozomi , Nishigori Chikako , O'Mahoney Paul , Manstein Dieter , Croft Rodney , Cole Curtis , de Gruijl Frank R. , Forbes Paul Donald , Trokel Stephen , Marshall John , Brenner David J. , Sliney David , Esvelt Kevin 
Published: November 2023

AbstractThe COVID‐19 pandemic underscored the crucial importance of enhanced indoor air quality control measures to mitigate the spread of respiratory pathogens. Far‐UVC is a type of germicidal ultraviolet technology, with wavelengths between 200 and 235 nm, that has emerged as a highly promising approach for indoor air disinfection. Due to its enhanced safety compared to conventional 254 nm upper‐room germicidal systems, far‐UVC allows for whole‐room direct exposure of occupied spaces, potentially offering greater efficacy, since the total room air is constantly treated. While current evidence supports using far‐UVC systems within existing guidelines, understanding the upper safety limit is critical to maximizing its effectiveness, particularly for the acute phase of a pandemic or epidemic when greater protection may be needed. This review article summarizes the substantial present knowledge on far‐UVC safety regarding skin and eye exposure and highlights research priorities to discern the maximum exposure levels that avoid adverse effects. We advocate for comprehensive safety studies that explore potential mechanisms of harm, generate action spectra for crucial biological effects and conduct high‐dose, long‐term exposure trials. Such rigorous scientific investigation will be key to determining safe and effective levels for far‐UVC deployment in indoor environments, contributing significantly to future pandemic preparedness and response.