bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of human placenta-derived exosome (pExo) as a potential osteoarthritis disease modifying therapeutic
Authors: Huang Chenfei , Zhao Yuechao , Lin Shengchen , Li Lin , Guo Xuan , Yumiseba Sebastian , Yang Jeng-dar , Hariri Robert , Ye Qian , He Shuyang , Kilcoyne Adrian 
Published: November 2023

Abstract
Objective
Human placenta-derived exosomes (pExo) were generated, characterized, and evaluated as a therapeutic candidate for the treatment of osteoarthritis (OA).

Methods
pExo was generated from full-term human placenta tissues by sequential centrifugation, purification, and sterile filtration. Upon analysis of particle size, cytokine composition, and exosome marker expression, pExo was further tested in cell-based assays to examine its effects on human chondrocytes. In vivo therapeutic efficacies were evaluated in a medial meniscal tear/medial collateral ligament tear (MCLT + MMT) rat model, in which animals received pExo injections intraarticularly and weight bearing tests during in-life stage while histopathology and immunohistochemistry were performed as terminal endpoints.

Results
pExo displayed typical particle size, expressed maker proteins of exosome, and contained proteins with pro-proliferative, pro-anabolic, anti-catabolic, or anti-inflammatory activities. In vitro, pExo promoted chondrocyte migration and proliferation dose-dependently, which may involve its activation of cell growth-related signaling pathways. Expression of inflammatory and catabolic genes induced in a cellular OA model was significantly suppressed by pExo. In the rat OA model, pExo alleviated pain burden, restored cartilage degeneration, and downregulated expressions of pro-inflammatory, catabolic, or apoptotic proteins in a dose-dependent manner.

Conclusions
Our study demonstrates that pExo has multiple potential therapeutic effects including symptom control and disease modifying characteristics. This may make it an attractive candidate for further development as an anti-OA therapeutic.
Moment inequalities for mixing long-span high-frequency data and strongly consistent estimation of OU integrated diffusion process
Authors: Yang Shanchao , Xie Jiaying , Luo Shuyi , Li Zhiyong , Yang Xin 
Published: November 2023

AbstractMixing is not much used in the high-frequency literature so far. However, mixing is a common weakly dependent property of continuous and discrete stochastic processes, such as Gaussian, Ornstein–Uhlenberck (OU), Vasicek, CIR, CKLS, logistic diffusion, generalized logistic diffusion, and double-well diffusion processes. So, long-span high-frequency data typically have weak dependence, and using mixing to study them is also an alternative approach. In this paper, we give some moment inequalities for long-span high-frequency data with ϕ-mixing, ρ-mixing, and α-mixing. These inequalities are effective tools for studying asymptotic properties. Applying these inequalities, we investigate the strong consistency of parameter estimation for the OU-integrated diffusion process. We also derive the mean square error of the estimation of the OU process and the optimal interval for the drift parameter estimator.
Effectiveness of tailored screening for multidrug-resistant organisms upon admission to an intensive care unit in the United Arab Emirates
Authors: Park Sungsoo , Kim Heesuk , Gatchalian Katrine K. , Oh Hyeyoung 
Published: November 2023

Abstract
Background
Multidrug-resistant organism (MDRO) screening may identify high-risk patients for MDRO infection and curb the spread of these resistant pathogens. However, the heterogeneous practices in MDRO screening and the diversity of MDRO risk factors necessitate a tailored approach for successful implementation. This study aimed to evaluate the performance of tailored MDRO screening in predicting MDRO carriage compared to universal screening.

Methods
Critically ill patients who underwent MDRO screening tests upon intensive care unit admission between September 2015 and December 2019 were included in the study. A risk-predicting model was developed using risk factors identified through multivariable logistic regression analysis. If an individual had one or more identified risk factors, the individual was deemed to be at risk of MDRO carriage and undergo tailored screening. The sensitivity of tailored screening was compared with universal screening for methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant Gram-negative bacilli (carbapenem-resistant Acinetobacter baumannii and carbapenem-resistant Enterobacterales).

Results
The use of tracheostomy or endotracheal tubes, previous antibiotic exposure, previous multidrug-resistant Gram-negative bacilli carriage history, admission to the medical department, peripheral vascular disease, and liver disease were associated with positive screening for multidrug-resistant Gram-negative bacilli. These six risk factors accounted for all positive screening for multidrug-resistant Gram-negative bacilli, requiring 38.6% of all tests. Notably, MRSA had different risk factor profiles, and the risk factor-based screening approach detected only 43.1% (31 out of 72) of MRSA-positive cases.

Conclusions
Tailored screening based on identified risk factors showed variable sensitivities to individual MDROs compared to universal screening. A tailored screening approach for individual MDROs may enhance the overall effectiveness of MDRO screening programs.
Fusion of sentinel-1 SAR and sentinel-2 MSI data for accurate Urban land use-land cover classification in Gondar City, Ethiopia
Authors: Dagne Shimelis Sishah , Hirpha Hurgesa Hundera , Tekoye Addisu Teshome , Dessie Yeshambel Barko , Endeshaw Adane Addis 
Published: November 2023

AbstractEffective urban planning and management rely on accurate land cover mapping, which can be achieved through the combination of remote sensing data and machine learning algorithms. This study aimed to explore and demonstrate the potential benefits of integrating Sentinel-1 SAR and Sentinel-2 MSI satellite imagery for urban land cover classification in Gondar city, Ethiopia. Synthetic Aperture Radar (SAR) data from Sentinel-1A and Multispectral Instrument (MSI) data from Sentinel-2B for the year 2023 were utilized for this research work. Support Vector Machine (SVM) and Random Forest (RF) machine learning algorithms were utilized for the classification process. Google Earth Engine (GEE) was used for the processing, classification, and validation of the remote sensing data. The findings of the research provided valuable insights into the performance evaluation of the Support Vector Machine (SVM) and Random Forest (RF) algorithms for image classification using different datasets, namely Sentinel 2B Multispectral Instrument (MSI) and Sentinel 1A Synthetic Aperture Radar (SAR) data. When applied to the Sentinel 2B MSI dataset, both SVM and RF achieved an overall accuracy (OA) of 0.69, with a moderate level of agreement indicated by the Kappa score of 0.357. For the Sentinel 1A SAR data, SVM maintained the same OA of 0.69 but showed an improved Kappa score of 0.67, indicating its suitability for SAR image classification. In contrast, RF achieved a slightly lower OA of 0.66 with Sentinel 1A SAR data. However, when the datasets of Sentinel 2B MSI and Sentinel 1A SAR were combined, SVM achieved an impressive OA of 0.91 with a high Kappa score of 0.80, while RF achieved an OA of 0.81 with a Kappa score of 0.809. These findings highlight the potential of fusing satellite data from multiple sources to enhance the accuracy and effectiveness of image classification algorithms, making them valuable tools for various applications, including land use mapping and environmental monitoring.
Relationship between the COVID-19 pandemic and structural inequalities within the pediatric trauma population
Authors: Georgeades Christina , Collings Amelia T. , Farazi Manzur , Bergner Carisa , Fallat Mary E. , Minneci Peter C. , Speck K. Elizabeth , Van Arendonk Kyle J. , Deans Katherine J. , Falcone Richard A. , Foley David S. , Fraser Jason D. , Gadepalli Samir K. , Keller Martin S. , Kotagal Meera , Landman Matthew P. , Leys Charles M. , Markel Troy A. , Rubalcava Nathan S. , St. Peter Shawn D. , Sato Thomas T. , Flynn-O’Brien Katherine T. ,   the Midwest Pediatric Surgery Consortium (MWPSC)
Published: November 2023

Abstract
Background
The COVID-19 pandemic disrupted social, political, and economic life across the world, shining a light on the vulnerability of many communities. The objective of this study was to assess injury patterns before and after implementation of stay-at-home orders (SHOs) between White children and children of color and across varying levels of vulnerability based upon children’s home residence.

Methods
A multi-institutional retrospective study was conducted evaluating patients < 18 years with traumatic injuries. A “Control” cohort from an averaged March-September 2016–2019 time period was compared to patients injured after SHO initiation-September 2020 (“COVID” cohort). Interactions between race/ethnicity or social vulnerability index (SVI), a marker of neighborhood vulnerability and socioeconomic status, and the COVID-19 timeframe with regard to the outcomes of interest were assessed using likelihood ratio Chi-square tests. Differences in injury intent, type, and mechanism were then stratified and explored by race/ethnicity and SVI separately.

Results
A total of 47,385 patients met study inclusion. Significant interactions existed between race/ethnicity and the COVID-19 SHO period for intent (p < 0.001) and mechanism of injury (p < 0.001). There was also significant interaction between SVI and the COVID-19 SHO period for mechanism of injury (p = 0.01). Children of color experienced a significant increase in intentional (COVID 16.4% vs. Control 13.7%, p = 0.03) and firearm (COVID 9.0% vs. Control 5.2%, p < 0.001) injuries, but no change was seen among White children. Children from the most vulnerable neighborhoods suffered an increase in firearm injuries (COVID 11.1% vs. Control 6.1%, p = 0.001) with children from the least vulnerable neighborhoods having no change. All-terrain vehicle (ATV) and bicycle crashes increased for children of color (COVID 2.0% vs. Control 1.1%, p = 0.04 for ATV; COVID 6.7% vs. Control 4.8%, p = 0.02 for bicycle) and White children (COVID 9.6% vs. Control 6.2%, p < 0.001 for ATV; COVID 8.8% vs. Control 5.8%, p < 0.001 for bicycle).

Conclusions
In contrast to White children and children from neighborhoods of lower vulnerability, children of color and children living in higher vulnerability neighborhoods experienced an increase in intentional and firearm-related injuries during the COVID-19 pandemic. Understanding inequities in trauma burden during times of stress is critical to directing resources and targeting intervention strategies.
An overview of fermentation in the food industry - looking back from a new perspective
Authors: Siddiqui Shahida Anusha , Erol Zeki , Rugji Jerina , Taşçı Fulya , Kahraman Hatice Ahu , Toppi Valeria , Musa Laura , Di Giacinto Giacomo , Bahmid Nur Alim , Mehdizadeh Mohammad , Castro-Muñoz Roberto 
Published: November 2023

AbstractFermentation is thought to be born in the Fertile Crescent, and since then, almost every culture has integrated fermented foods into their dietary habits. Originally used to preserve foods, fermentation is now applied to improve their physicochemical, sensory, nutritional, and safety attributes. Fermented dairy, alcoholic beverages like wine and beer, fermented vegetables, fruits, and meats are all highly valuable due to their increased storage stability, reduced risk of food poisoning, and enhanced flavor. Over the years, scientific research has associated the consumption of fermented products with improved health status. The fermentation process helps to break down compounds into more easily digestible forms. It also helps to reduce the amount of toxins and pathogens in food. Additionally, fermented foods contain probiotics, which are beneficial bacteria that help the body to digest food and absorb nutrients. In today’s world, non-communicable diseases such as cardiovascular disease, type 2 diabetes, cancer, and allergies have increased. In this regard, scientific investigations have demonstrated that shifting to a diet that contains fermented foods can reduce the risk of non-communicable diseases. Moreover, in the last decade, there has been a growing interest in fermentation technology to valorize food waste into valuable by-products. Fermentation of various food wastes has resulted in the successful production of valuable by-products, including enzymes, pigments, and biofuels.
Assessing the Prevalence of Doping Among Elite Athletes: An Analysis of Results Generated by the Single Sample Count Method Versus the Unrelated Question Method
Authors: Ulrich Rolf , Cléret Léa , Comstock R. Dawn , Kanayama Gen , Simon Perikles , Pope Harrison G. 
Published: November 2023

AbstractIn 2011, a group of researchers investigated the 12-month prevalence of doping at the 13th International Association of Athletics Federations World Championships in Athletics (WCA) in Daegu, South Korea, and also at the 12th Pan-Arab Games (PAG) in Doha, Qatar. The prevalence of doping at each event was estimated using an established randomized response method, the Unrelated Question Model (UQM). The study, published in 2018, found that the prevalence of past-year doping was at least 30% at WCA and 45% at PAG. At both events, separate data sets were collected in addition to the UQM data using a new method, the single sample count (SSC). Recently, Petróczi et al. have reported 12-month doping prevalence estimates for these two events based on the SSC data. These investigators obtained substantially lower prevalence estimates using the SSC and suggested that the 2018 estimates based on the UQM may have been too high. However, in this communication, we point out several possible shortcomings in the methods of Petróczi et al. and show that their SSC data would be equally compatible with a high 12-month doping prevalence comparable to the UQM estimates published in 2018.
Living in a large family and low daily water consumption substantially expose for human scabies in rural Ethiopia: a matched analysis
Authors: Ayele Agernesh , Adane Metadel , Adane Balew , Berihun Gete , Gebrehiwot Mesfin , Woretaw Lebasie , Berhanu Leykun , Atanaw Getu , Feleke Hailemariam , Moges Mekonnen , Tegegne Eniyew , Azanaw Jember , Malede Asmamaw 
Published: November 2023

Abstract
Background
Scabies has been added to the neglected tropical diseases portfolio for large-scale disease control action since 2017 and is part of the WHO roadmap for NTDs 2021–2030, targeted at ending the neglect to achieve the sustainable development goals. Previous studies have not fitted matched analysis to identify predictors of scabies infestation in Ethiopia. Information is also scarce about predictors of scabies infestation in this area. Therefore, this study aimed to identify predictors of scabies infestation in rural Aneded District, northwest Ethiopia.

Methods
A community-based matched case–control study involving 183 cases and 549 controls was undertaken from March 1 to May 31, 2021, in rural Aneded District. A two-stage sampling technique with a house-to-house census for the screening of scabies cases was employed. A structured questionnaire with questions on sociodemographics, behavior, water supply, sanitation, and hygiene, and delivery of scabies-specific interventions was used. Pretesting, training of data collectors and supervisors, and supervision were applied to keep the data quality. A multivariable conditional logistic regression model was fitted to identify predictors of scabies.

Results
Unmarried individuals or those in separated families (adjusted matched odds ratio (AmOR = 2.71; 95% CI 1.30–5.65); those unable to read and write or in illiterate families (AmOR = 5.10; 95% CI 1.81–14.36); those in large families (AmOR = 6.67; 95% CI 2.83–15.73); households that had longer travel times for water collection (AmOR = 2.27; 95% CI 1.08–4.76); those that had low daily water consumption (AmOR = 6.69; 95% CI 2.91–15.37); households that disposed of solid wastes in open fields (AmOR = 5.60; 95% CI 2.53–12.40); and households that did not receive scabies-specific interventions (AmOR = 2.98; 95% CI 1.39–6.39) had increased odds of scabies.

Conclusions
Being unmarried, illiteracy, large family, long travel time for water collection, low daily water consumption, open dumping of solid wastes, and inaccessibility of scabies-specific interventions are predictors of scabies. This information is instrumental for redesigning improved scabies-specific interventions that consider educational status, marital status, family size, water collection time, daily water consumption, solid waste disposal, and equity and optimization in delivering existing interventions in rural Ethiopia.
Safety and immunogenicity of influenza A(H3N2) component vaccine in juvenile systemic lupus erythematosus
Authors: Aikawa Nadia Emi , Borba Eduardo Ferreira , Balbi Verena Andrade , Sallum Adriana Maluf Elias , Buscatti Izabel Mantovani , Campos Lucia Maria Arruda , Kozu Kátia Tomie , Garcia Cristiana Couto , Capão Artur Silva Vidal , de Proença Adriana Coracini Tonacio , Leon Elaine Pires , da Silva Duarte Alberto José , Lopes Marta Heloisa , Silva Clovis Artur , Bonfá Eloisa 
Published: November 2023

Abstract
Introduction
Seasonal influenza A (H3N2) virus is an important cause of morbidity and mortality in the last 50 years in population that is greater than the impact of H1N1. Data assessing immunogenicity and safety of this virus component in juvenile systemic lupus erythematosus (JSLE) is lacking in the literature.

Objective
To evaluate short-term immunogenicity and safety of influenza A/Singapore (H3N2) vaccine in JSLE.

Methods
24 consecutive JSLE patients and 29 healthy controls (HC) were vaccinated with influenza A/Singapore/INFIMH-16-0019/2016(H3N2)-like virus. Influenza A (H3N2) seroprotection (SP), seroconversion (SC), geometric mean titers (GMT), factor increase in GMT (FI-GMT) titers were assessed before and 4 weeks post-vaccination. Disease activity, therapies and adverse events (AE) were also evaluated.

Results
JSLE patients and controls were comparable in current age [14.5 (10.1–18.3) vs. 14 (9–18.4) years, p = 0.448] and female sex [21 (87.5%) vs. 19 (65.5%), p = 0.108]. Before vaccination, JSLE and HC had comparable SP rates [22 (91.7%) vs. 25 (86.2%), p = 0.678] and GMT titers [102.3 (95% CI 75.0–139.4) vs. 109.6 (95% CI 68.2–176.2), p = 0.231]. At D30, JSLE and HC had similar immune response, since no differences were observed in SP [24 (100%) vs. 28 (96.6%), p = 1.000)], SC [4 (16.7%) vs. 9 (31.0%), p = 0.338), GMT [162.3 (132.9–198.3) vs. 208.1 (150.5–287.8), p = 0.143] and factor increase in GMT [1.6 (1.2–2.1) vs. 1.9 (1.4–2.5), p = 0.574]. SLEDAI-2K scores [2 (0–17) vs. 2 (0–17), p = 0.765] and therapies remained stable throughout the study. Further analysis of possible factors influencing vaccine immune response among JSLE patients demonstrated similar GMT between patients with SLEDAI < 4 compared to SLEDAI ≥ 4 (p = 0.713), as well as between patients with and without current use of prednisone (p = 0.420), azathioprine (p = 1.0), mycophenolate mofetil (p = 0.185), and methotrexate (p = 0.095). No serious AE were reported in both groups and most of them were asymptomatic (58.3% vs. 44.8%, p = 0.958). Local and systemic AE were alike in both groups (p > 0.05).

Conclusion
This is the first study that identified adequate immune protection against H3N2-influenza strain with additional vaccine-induced increment of immune response and an adequate safety profile in JSLE. (www.clinicaltrials.gov, NCT03540823).
Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury
Authors: Wang Yunhang , Su Hong , Zhong Juan , Zhan Zuxiong , Zhao Qin , Liu Yuan , Li Sen , Wang Haiyan , Yang Ce , Yu Lehua , Tan Botao , Yin Ying 
Published: November 2023

AbstractIn this study, we examined the combined impact of osteopontin (OPN) and treadmill training on mice with spinal cord injury (SCI). OPN was overexpressed by injecting AAV9-SPP1-GFP into the sensorimotor cortex, followed by a left incomplete C5 crush injury two weeks later. Mice (Ex or Ex + OPN group) were trained at 50% maximum running speed for 8 weeks. To analyze the effects, we used biotinylated dextran amine (BDA) for tracing the corticospinal tract (CST) and performed Western blotting and immunohistochemical methods to assess the activation of the mammalian target of rapamycin (mTOR). We also examined axonal regeneration and conducted behavioral tests to measure functional recovery. The results demonstrated that treadmill training promoted the expression of neurotrophic factors such as brain-derived neurotrophic factor (BNDF) and insulin-like growth factor I (IGF-1) and activated mTOR signaling. OPN amplified the effect of treadmill training on activating mTOR signaling indicated by upregulated phosphorylation of ribosomal protein S6 kinase (S6). The combination of OPN and exercise further promoted functional recovery and facilitated limited CST axonal regeneration which did not occur with treadmill training and OPN treatment alone. These findings indicate that OPN enhances the effects of treadmill training in the treatment of SCI and offer new therapeutic insights for spinal cord injury.
Hindbrain Adrenergic/Noradrenergic Control of Integrated Endocrine and Autonomic Stress Responses
Authors: Pace Sebastian A , Myers Brent 
Published: November 2023

Abstract
Hindbrain adrenergic/noradrenergic nuclei facilitate endocrine and autonomic responses to physical and psychological challenges. Neurons that synthesize adrenaline and noradrenaline target hypothalamic structures to modulate endocrine responses while descending spinal projections regulate sympathetic function. Furthermore, these neurons respond to diverse stress-related metabolic, autonomic, and psychosocial challenges. Accordingly, adrenergic and noradrenergic nuclei are integrative hubs that promote physiological adaptation to maintain homeostasis. However, the precise mechanisms through which adrenaline- and noradrenaline-synthesizing neurons sense interoceptive and exteroceptive cues to coordinate physiological responses have yet to be fully elucidated. Additionally, the regulatory role of these cells in the context of chronic stress has received limited attention. This mini-review consolidates reports from preclinical rodent studies on the organization and function of brainstem adrenaline and noradrenaline cells to provide a framework for how these nuclei coordinate endocrine and autonomic physiology. This includes identification of hindbrain adrenaline- and noradrenaline-producing cell groups and their role in stress responding through neurosecretory and autonomic engagement. Although temporally and mechanistically distinct, the endocrine and autonomic stress axes are complementary and interconnected. Therefore, the interplay between brainstem adrenergic/noradrenergic nuclei and peripheral physiological systems is necessary for integrated stress responses and organismal survival.
Yi Ok 李鈺 and His Iŏn 俚諺 (Folk Vernacular)
Authors: Oh Young Kyun 
Published: November 2023

Abstract
Yi Ok 李鈺 (1760–1815) was a prolific writer who lived in Hanyang (modern Seoul) during the late eighteenth and early nineteenth centuries. Among the massive pile of writings he left behind, his Iŏn 俚諺 (Folk Vernacular) best reveals his broad and multifaceted linguistic and literary knowledge, which in turn epitomizes the cultural complexity of late Chosŏn. In its three introductory treatises, as well as in the ensuing sixty-six pentasyllabic Sinitic quatrains written in female voices, Yi Ok illustrates why and how he writes poems about how “heaven and earth and the ten thousand things” (ch’ŏnji manmul 天地萬物) speak through him. This article combines a scholarly introduction to Yi Ok's life and oeuvre with a philological translation of his Iŏn that unpacks the complexity of Yi Ok's age to gain a fuller understanding of the last stage of Literary Sinitic (hanmun) literature in traditional Korea.