bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of mebendazole polymorphs by Fourier transform IR spectrometry using chemometric methods
Authors: Aboul‐Enein Hassan Y. , Bunaciu Andrei A. , Fleschin Serban 
Published: October 2004

AbstractA diffuse reflectance IR Fourier transform IR spectrometry (DRIFTS) method was developed for the rapid, direct measurement of mebendazole in drugs. Conventional KBr spectra and DRIFTS spectra were compared for the best determination of the active substance in the drug formulations. Two chemometric approaches were used in the data processing: multicomponent partial least squares (PLS2) and principal component regression. The best results were obtained with the PLS2 method. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 56–60, 2002; DOI 10.1002/bip.10041
Circular dichroism study of interactions of Fungizone or AmBisome forms of amphotericin B with human low density lipoproteins
Authors: Barwicz Joanna , Beauregard Marc , Tancrède Pierre 
Published: October 2004

AbstractAmphotericin B (AmB), a potent antifungal agent used to treat invasive fungal infections, is still employed more than 40 years after its introduction in the pharmacopea. When injected into the blood stream, this antibiotic is carried by low density lipoproteins (LDLs) to which it induces the formation of oxidation products responsible in part for some of the severe adverse effects of the drug. However, the oxidative damages induced to LDLs are not yet understood. We present here the effects of the Fungizone and AmBisome forms of AmB on LDLs as compared to those of CuSO4, a well‐known powerful oxidant of LDLs. We use circular dichroism (CD) spectroscopy, which is particularly useful because it allows the investigation of the structural integrity of the proteic moiety of LDL upon interaction with AmB. The CD spectra also yield information on the drug itself because in its oligomer form it presents a strong dichroic signal in a spectral region different from that of the protein. Our results show that neither form of AmB changes the secondary structure of the protein while the helical content of the LDL is increased either in the presence of CuSO4 alone or in the presence of CuSO4 and AmBisome or Fungizone. On the other hand, the CD spectra of the antibiotic indicate that Fungizone AmB suffers important oxidative damage in the presence of LDLs and CuSO4 while this damage is not present with AmBisome AmB. These observations lead us to propose that the structural modifications of the proteic part of LDLs induced by the Cu2+ ions are involved in the important oxidative damage suffered by Fungizone AmB, which in this form is much more susceptible to interaction with its environment than AmBisome. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 49–55, 2002; DOI 10.1002/bip.10042
Spectroscopic studies and PM3 semiempirical calculations of Schiff bases of gossypol with L‐amino acid methyl esters
Authors: Przybylski Piotr , Brzezinski Bogumil 
Published: October 2004

AbstractThree Schiff bases of racemic gossypol with L‐amino acid methyl esters are synthesized and studied by FTIR and 1H‐NMR spectroscopy, and their structures are calculated by the PM3 semiempirical method. The Schiff bases in the study exist in the solid state and in solutions as enamine forms. The existence of diastereoisomers is very visible in the 1H‐NMR spectra. The amount of the diastereoisomers depends on the amount of time the solutions are rested in diffused light. The epimerization from D,L‐isomer to L,L‐isomer is very slow. The structures of the Schiff bases and the hydrogen bonds within these structures are discussed. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 61–69, 2002; DOI 10.1002/bip.10043
Interaction between Btk TH and SH3 domain
Authors: Okoh Michael P. , Vihinen Mauno 
Published: October 2004

AbstractSeveral mechanisms are involved in the regulation of cellular signaling. Bruton tyrosine kinase (Btk) of the Tec family contains in the Tec homology (TH) domain a proline‐rich region (PRR) capable of interacting with several SH3 domains. The Btk has the SH3 domain adjacent to the TH domain. CD and fluorescence spectroscopy were used to study the binding of two peptides corresponding to segments in the PRR to the Btk SH3 domain. The peptide for the N‐terminal half of the PRR binds specifically, whereas the other peptide had hardly any affinity. The TH domain has about four times lower affinity to the SH3 domain than the peptide, 17.0 vs 3.9 μM. The interaction was further tested with an SH3 domain construct that contained the PRR. The two peptides cannot compete for the binding to the extended protein and the TH domain has two times lower affinity to the extended SH3 domain. The intra‐ or intermolecular interaction between the TH and SH3 domain might have regulatory function also in the other Tec family members. © 2002 Wiley Periodicals, Inc. Biopolymers 63: 325–334, 2002
Fourier transform‐Raman spectroscopic study of natural resins of archaeological interest
Authors: Brody Rachel H. , Edwards Howell G. M. , Pollard A. Mark 
Published: October 2004

AbstractResins from several different genera are studied using Fourier transform (FT)‐Raman spectroscopy. Tree resins can be broadly divided into those that contain diterpenoid components and those that contain triterpenoid components. The diterpenoid resins analyzed are from the genera Pinus, Cedrus, and Agathis (kauri resin) and the triterpenoid resins examined are samples from Pistacia, Boswellia (frankincense), and Commiphora (myrrh) genera. A protocol is developed to nondestructively distinguish diterpenoid and triterpenoid resins and to differentiate the genera within the two types. The effects of oxidation on the discrimination of the FT‐Raman spectra are considered. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 129–141, 2002
Medical applications of Raman spectroscopy: From proof of principle to clinical implementation
Authors: Choo‐Smith L.‐P. , Edwards H. G. M. , Endtz H. P. , Kros J. M. , Heule F. , Barr H. , Robinson J. S. , Bruining H. A. , Puppels G. J. 
Published: October 2004

AbstractRaman spectroscopy has recently been applied ex vivo and in vivo to address various biomedical issues such as the early detection of cancers, monitoring of the effect of various agents on the skin, determination of atherosclerotic plaque composition, and rapid identification of pathogenic microorganisms. This leap in the number of applications and the number of groups active in this field has been facilitated by several technological advancements in lasers, CCD detectors, and fiber‐optic probes. However, most of the studies are still at the proof of concept stage. We present a discussion on the status of the field today, as well as the problems and issues that still need to be resolved to bring this technology to hospital settings (i.e., the medical laboratory, surgical suites, or clinics). Taken from the viewpoint of clinicians and medical analysts, the potential of Raman spectroscopic techniques as new tools for biomedical applications is discussed and a path is proposed for the clinical implementation of these techniques. © 2002 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 67: 1–9, 2002; DOI 10.1002/bip.10064
Preferred conformations of RGDX tetrapeptides to inhibit the binding of fibrinogen to platelets
Authors: Park Hae Sook , Kim Choonmi , Kang Young Kee 
Published: October 2004

AbstractThe conformational study on Arg–Gly–Asp (RGD)‐containing tetrapeptides in the unhydrated and hydrated states has been carried out using the force field ECEPP/3 and the hydration shell model. The tetrapeptides studied here are H–RGDX–OH (X = Trp, Tyr, Phe, Leu, Val, Cys, Gln, and Ser), which show the inhibitory activity for binding of fibrinogen to platelets in the order of RGDW ≈ RGDY ≈ RGDF ≈ RGDL > RGDV ≥ RGDC ≥ RGDQ ≥ RGDS. The backbone conformations with two C7 backbone‐to‐backbone hydrogen bonds between Asp and Arg residues and between Xaa and Gly residues are in common most probable for the RGD sequence of RGDX tetrapeptides in the hydrated state. The dominant β‐turns for RGDX are found to be the types V′ and IV at Gly–Asp and Asp–Xaa sequences, respectively, which are quite similar to the types II′ and I (or II), respectively. However, it cannot be ruled out that the extended conformations are also remarkably feasible for RGDX tetrapeptides in water by peering the distributions of backbone conformations. These calculated results are consistent with the experimental results on RGD‐containing proteins and conformationally constrained RGD‐containing peptides. The reason why the RGDX becomes more potent as the side chain of the X residue is more hydrophobic may be ascribed to that the more hydrophobic is the residue X, the more populated are β‐turn structures for the Gly–Asp sequence. The hydrophobic side chain of X residue exposed to water is likely to interact with the hydrophobic region of receptor easily. © 2002 Wiley Periodicals, Inc. Biopolymers 63: 298–313, 2002
Synthesis, conformational analysis, and spectroscopic characterization of peptides based on Daf, the first rigid transition‐metal receptor, cyclic Cα,α‐disubstituted glycine
Authors: Peggion Cristina , Crisma Marco , Formaggio Fernando , Toniolo Claudio , Wright Karen , Wakselman Michel , Mazaleyrat Jean‐Paul 
Published: October 2004

AbstractThree series of terminally protected model oligopeptides to the nonamer level, based on 9‐amino‐4,5‐diazafluorene‐9‐carboxylic acid, the first rigid bipyridine‐type Cα,α‐disubstituted glycine, and either Gly, L‐Ala, or Aib residues were synthesized by solution methods and fully characterized. The molecular structures of two derivatives and one tripeptide were determined in the crystal state by x‐ray diffraction. Moreover, the solution preferred conformations of these peptides were assessed by Fourier transform infrared absorption and 1H‐NMR techniques. A comparison with the known structural tendencies of the strictly related Cα,α‐disubstituted glycyl residues 1‐aminocyclopentane‐1‐carboxylic acid and 9‐aminofluorene‐9‐carboxylic acid is made, and the implications for the use of the 9‐amino‐4,5‐diazafluorene‐9‐carboxylic acid residue in conformationally constrained analogs of bioactive peptides are briefly examined. A spectroscopic (uv absorption, fluorescence, CD) characterization of this novel heteroaromatic Cα,α‐disubstituted glycine is also reported. Finally, preliminary conformational data and membrane activity measurements are discussed for an analog of the lipopeptaibol antibiotic [L‐Leu11–OMe] trichogin GA IV in which a 9‐amino‐4,5‐diazafluorene‐9‐carboxylic acid residue was synthetically incorporated in position 1 (replacing the original Aib residue). © 2002 Wiley Periodicals, Inc. Biopolymers 63: 314–324, 2002
Time‐resolved FTIR difference spectroscopy as tool for investigating refolding reactions of ribonuclease T1 synchronized with trans → cis prolyl isomerization
Authors: Moritz Ralf , Reinstädler Diane , Fabian Heinz , Naumann Dieter 
Published: October 2004

AbstractThe structurally well‐characterized enzyme ribonuclease T1 was used as a model protein to further evaluate time‐resolved Fourier transform IR difference spectroscopy in conjunction with temperature‐jump techniques as a useful detection technique for protein folding studies. Compared to the wild‐type protein, it was confirmed that the lack of one cis‐proline bond at position 55 of the S54G/P55N variant is sufficient to significantly simplify and accelerate the refolding process. This result was sustained by the characterization of the early refolding events that occurred within the experimental dead time. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 145–155, 2002
Analysis of peptide rotational diffusion by homonuclear NMR
Authors: Malliavin Thérèse E. , Giudice Emmanuel 
Published: October 2004

AbstractThe analysis of the rotational diffusion of a molecule using homonuclear NMR is investigated. The homonuclear longitudinal and transverse cross‐relaxation rates, which can be quantitatively measured using off‐Resonance Rotating frame nuclear Overhauser Effect Spectroscopy (ROESY), are used to build a distribution, which exhibits a solid‐state‐like pattern characteristic of the diffusion tensor. The distributions of the antimicrobial peptide ranalexin in water and in 30% of trifluoracetic acid (TFE) are compared, and the peptide rotational diffusion is shown to be more isotropic in water than in 30% TFE. This difference is further supported by the analysis of NMR ranalexin conformers in 30% TFE, and by the analysis of a molecular dynamics simulation of peptide in water. © 2002 Wiley Periodicals, Inc. Biopolymers 63: 335–342, 2002
Stability studies of recombinant Saccharomyces cerevisiae in the presence of varying selection pressure
Authors: Gupta Jagdish C. , Mukherjee K. J. 
Published: October 2004

AbstractA recombinant yeast plasmid carrying the Ieu2 gene for auxotrophic complementation and a reporter gene for β‐galactosidase under the control of Gal10 promoter was studied in Saccharomyces cerevisiae. Growth, product formation, and plasmid stability were studied in defined, semi‐defined, and complex media. The biomass concentration and specific activity were higher in complex medium than in defined medium, which was selective for the growth of plasmid‐containing cells, leading to a 10‐fold increase in volumetric activity. However, plasmid instability was very high in complex media with 50% plasmid‐free cells emerging in the culture within 75 h of cultivation. In order to control instability, the growth rates of the plasmid‐containing and plasmid‐free cells were determined in semi‐defined media, which consisted of defined medium supplemented with different concentrations of yeast extract. Below a critical concentration of yeast extract (0.05 g/L), the plasmid‐containing cells had a growth rate advantage over the plasmid‐free cells. This was possibly because, at this concentration of yeast extract, the availability of leucine became the rate‐determining factor in the specific growth rate of plasmid‐free cells. A feeding strategy was designed which maintained a low concentration of the residual yeast extract in the medium and thus continuously provided the plasmid‐containing cells with a competitive advantage over the plasmid‐free cells. This resulted in high stability as well as high cell density under non‐selective conditions, which led to a 10‐fold increase in the volumetric activity compared to that achieved in defined selective media. A simple mathematical model was formulated to verify the experimental data. The important state variables and process parameters, i.e., biomass concentration, β‐galactosidase expression, sucrose consumption, yeast extract consumption, and specific growth rates of the two cell populations, were evaluated. These variables and parameters along with the differential equations based on material balances as well as the experimental results obtained were used in a mathematical model for the fed‐batch cultivation. These correctly verified the experimental data and clearly illustrated the concept behind the success of the fed‐batch strategy under yeast extract starvation. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 475–488, 2002.
Mechanism of adaptation of an atypical alkaline p‐nitrophenyl phosphatase from the archaeon Halobacterium salinarum at low‐water environments
Authors: Marhuenda‐Egea Frutos C. , Piera‐Velázquez Sonsoles , Cadenas Chiquinquirá , Cadenas Eduardo 
Published: October 2004

AbstractEnzymes suspended in organic solvents represent a versatile system for studying the involvement of water in catalytic properties and their flexibility in adapting to different environmental conditions. The extremely halophilic alkaline p‐nitrophenylphosphate phosphatase from the archaeon Halobacterium salinarum was solubilized in an organic medium consisting of reversed micelles of hexadecyltrimethylammoniumbromide in cyclohexane, with 1‐butanol as cosurfactant. Hydrolysis of p‐nitrophenylphosphate was nonlinear with time when the enzyme was microinjected into reversed micelles that contained substrate. These data are consistent with a kinetic model in which the enzyme is irreversibly converted from an initial form to a final stable form during the first seconds of the encapsulation process. The model features a rate constant (k) for that transition and separate hydrolysis rates, v1 and v2, for the two forms of the enzyme. The enzyme conversion may be governed by the encapsulation process. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 497–502, 2002.
Microbial synthesis of semiconductor CdS nanoparticles, their characterization, and their use in the fabrication of an ideal diode
Authors: Kowshik Meenal , Deshmukh Neelima , Vogel W. , Urban J. , Kulkarni S. K. , Paknikar K. M. 
Published: October 2004

AbstractCadmium sulfide nanoparticles were synthesized intracellularly by a Schizosaccharomyces pombe strain when challenged with 1 mM cadmium in solution. The nanoparticles, a known semiconducting material, exhibited an absorbance maximum at 305 nm. X‐ray scattering data showed that the nanoparticles had a Wurtzite (Cd16S20)‐type hexagonal lattice structure and most of the nanoparicles were in the size range of 1–1.5 nm. The nanoparticles were used in the fabrication of a heterojunction with poly (p‐phenylenevinylene). The diode exhibited ∼75 mA/cm2 current at 10 V when forward biased and the breakdown occurred at ∼15 V in the reverse biased mode. These characteristics are considered ideal for a diode. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 583–588, 2002.
Production of wild‐type and peptide fusion cutinases by recombinant Saccharomyces cerevisiae MM01 strains
Authors: Calado Cecília R. C. , Mannesse Maurice , Egmond Maarten , Cabral Joaquim M. S. , Fonseca Luis P. 
Published: October 2004

AbstractThis study focused on the growth of Saccha‐romyces cerevisiae MM01 recombinant strains and the respective production of three extracellular heterologous cutinases: a wild‐type cutinase and two cutinases in which the primary structure was fused with the peptides (WP)2 and (WP)4, respectively. Different cultivation and strategies were tested in a 2‐L shake flask and a 5‐L bioreactor, and the respective cell growth and cutinase production were analyzed and compared for the three yeast strains. The highest cutinase productions and productivities were obtained in the fed‐batch culture, where wild‐type cutinase was secreted up to a level of cutinase activity per dry cell weight (specific cell activity) of 4.1 Umg−1 with activity per protein broth (specific activity) of 266 Umg−1, whereas cutinase‐(WP)2 was secreted with a specific cell activity of 2.1 Umg−1 with a specific activity of 200 Umg−1, and cutinase‐(WP)4 with a specific cell activity of 0.7 Umg−1 with a specific activity of 15 Umg−1. The results indicate that the fusion of hydrophobic peptides to cutinase that changes the physical properties of the fused protein limits cutinase secretion and subsequently leads to a lower plasmid stability and lower yeast cell growth. These effects were observed under different cultivation conditions (shake flask and bioreactor) and cultivation strategies (batch culture versus fed‐batch culture). © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 692–698, 2002.
A case of acute myocardial infarction treated with a new thrombectomy system
Authors: Nishida Takahiro , Nakamura Masato , Tsunoda Taro , Iijima Raisuke , Shiba Masanori , Wada Masamichi , Nakajima Rintaro , Kitagawa Yoko , Yajima Suguru , Wakayama Megumi , Shibuya Kazutoshi , Yamaguchi Tetsu 
Published: October 2004

AbstractThrombotic occlusion in the culprit lesion of acute myocardial infarction was successfully recanalized using a 4.5 Fr thrombectomy catheter (RESCUE), which was also used to normalize the coronary blood flow. The retrieved specimens were white thrombi containing red thrombi and cholesterol crystals, indicating plaque rupture. Cathet Cardiovasc Intervent 2002;55:239–243. © 2002 Wiley‐Liss, Inc.
Intravascular ultrasound evaluation of ruptured plaque in the left main coronary artery misinterpreted as an aneurysm by angiography
Authors: Li Yuxin , Honye Junko , Takayama Tadateru , Saito Satoshi 
Published: October 2004

AbstractWe report a patient who demonstrated a left main coronary aneurysm by angiography. Intravascular ultrasound (IVUS) revealed that it was in fact an ulceration, which indicated ruptured plaque. This case provides evidence that IVUS can permit a more powerful definition of ruptured plaque than angiography. On IVUS, ulceration exhibits significantly different characteristics from aneurysm. Cathet Cardiovasc Interv 2004;63:314–316. © 2004 Wiley‐Liss, Inc.
Gadolinium‐based balloon angioplasty for pulmonary artery stenosis in an infant with a right isomerism
Authors: Sugiyama Hisashi , Kadono Toshie , Hoshiai Minako , Tan Tetsushi , Koizumi Keiichi , Sakamoto Hajime , Nakazawa Simpei 
Published: October 2004

AbstractWe report here the first described case of utilizing gadolinium‐based contrast material as the contrast agent during a catheter intervention treatment for pulmonary artery stenosis. The patient, a male infant with complex heart disease associated with a right isomerism, had a history of severe allergic reaction to iodine‐containing contrast agents. A combination of digital subtraction angiography and a gadolinium contrast agent, however, provided us with good‐quality images both before and after balloon angioplasty without any associated complications. This method should therefore be considered as an alternative angiographic procedure in children with a high risk of iodine‐related allergic complications. Catheter Cardiovasc Interv 2004;63:346–350. © 2004 Wiley‐Liss, Inc.
Atypical hemodynamic manifestations of cardiac tamponade
Authors: Sharma Naveen , Panchal Vipul , Kalaria Vijay G. 
Published: October 2004

AbstractClinical examination and transthoracic echocardiography play a vital role in the management of patients with pericardial effusion and cardiac tamponade physiology. We report patients in advanced phase 3 cardiac tamponade with variant clinical and hemodynamic presentations. These atypical cardiac tamponade cases include: A patient with severe aortic valve regurgitation who lacked pulsus paradoxus; a patient with systemic sclerosis without hypotension; and a patient with pulmonary hypertension lacking right heart collapse on echocardiography. Recognition of these atypical clinical and hemodynamic manifestations of cardiac tamponade will avoid undue delay in the treatment. Catheter Cardiovasc Interv 2004;63:339–345. © 2004 Wiley‐Liss, Inc.