bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new approach towards extracorporeal gas exchange and first in vitro results
Authors: Mouzakis Foivos Leonidas , Kashefi Ali , Spillner Jan , Rütten Stephan , Mottaghy Khosrow , Hima Flutura 
Published: November 2023

Abstract

Objectives
Extracorporeal life support (ECLS) pertains to therapeutic and prophylactic techniques utilized in a wide range of medical applications, with severe pulmonary diseases being the most prominent cases. Over the past decades, little progress has been made in advancing the basic principles and properties of gas exchangers. Here, in an unconventional approach, dialysis hollow fibers are handled with silicone to create a purely diffusive coating that prevents plasma leakage and promotes gas exchange.

Methods
Commercial dialyzers of varying surface area and fiber diameter have been coated with silicone, to determine the impact of each parameter on performance. The impermeability of the silicone layer has been validated by pressurization and imaging methods. SEM images have revealed a homogeneous silicone film coating the lumen of the capillaries, while fluid dynamic investigations have confirmed its purely diffusive nature.

Results
The hemodynamic behavior and the gas exchange efficiency of the silicone-coated prototypes have been investigated in vitro with porcine blood under various operating conditions. Their performance has been found to be similar to that of a commercial PMP oxygenator.

Conclusions
This novel class of gas exchangers is characterized by high versatility and expeditious manufacturing. Intraoperability between conventional ECLS systems and dialysis machines broadens the range of application infinitely. Ultimately, long-term clinical applicability ought to be determined over in vivo animal investigations.
An IoT-based intelligent smart energy monitoring system for solar PV power generation
Authors: Krishna Rao Challa , Sahoo Sarat Kumar , Yanine Franco Fernando 
Published: November 2023

Abstract
As the world’s attention turns to cleaner, more dependable, and sustainable resources, the renewable energy sector is rising quickly. The decline in world energy use and climate change are the two most significant factors nowadays. PV forecasting was essential to enhancing the efficiency of the real-time control system and preventing any undesirable effects. The smart energy management systems of distributed energy resources, the forecasting model of irradiation received from the sun, and therefore PV energy production might mitigate the impact of uncertainty on PV energy generation, improve system dependability, and increase the incursion level of solar power generation. Smart sensors and Internet of Things technologies are essential for monitoring and controlling applications in a broad range of fields. As a result, solar power generation forecasting was essential for microgrid stability and security, as well as solar photovoltaic integration in a strategic approach. This paper examines how to use IoT, a solar photovoltaic system being monitored, and shows the proposed monitoring system is a potentially viable option for smart remote and in-person monitoring of a solar PV system.
Development and user acceptability testing of healthy heart mobile application – a tool for cardiovascular risk modification among patients with type 2 diabetes mellitus
Authors: Erande Prithviraj , Ray Mohanty Ipseeta , Rai Sandeep 
Published: November 2023

Abstract

Objectives
Cardiovascular disease (CVD) remains the primary cause of mortality in individuals with type 2 diabetes mellitus. Digital health has quickly emerged as a technology with the ability to bridge the gap in cardiovascular disease self-management and revolutionize the way healthcare has traditionally been delivered. However, there is little data on the application of mobile technologies for cardiovascular risk reduction among diabetic patients. The current study has been constructed with this in mind.

Methods
A framework for the development of a healthy heart mobile application for CVD risk stratification and risk management among Type 2 diabetes mellitus patients was finalized after consultation with diabetologists, nutritionists, and scientists. The mobile app has three user cases: Patient login, doctor login, and admin login. A questionnaire was designed and the feedback of patients and Physicians was taken regarding the design, presentation, content, and user-friendliness of the app based on responses obtained on the questionnaire.

Results
The Android version of the healthy heart mobile mobile app was developed for CVD risk stratification and risk management among type 2 diabetes mellitus patients. The dashboard of the mobile app displayed the CVD risk score and category (mild, moderate, high, or very high CVD risk; which was colored coded), health tracker to monitor medication adherence, lipid profile, diabetes control, CVD risk profile and compliance with the WHO recommendations regarding diet, physical activity and addictions, User acceptability and experience were tested for the developed healthy heart mobile app among patients and physicians. The majority of the respondents graded the design, presentation, content, and user-friendliness of the app as either excellent or good.

Conclusions
The mobile app for self-management and CVD risk reduction among diabetic patients was successfully developed. The paper and mobile-based CVD risk calculation and stratification were found to be a match for all the participants. The app was updated based on suggestions from the pilot study and was well-accepted by both patients and physicians.
In vitro studies of titanium dioxide nanoparticles modified with glutathione as a potential drug delivery system
Authors: Pulit-Prociak Jolanta , Długosz Olga , Staroń Anita , Radomski Piotr , Domagała Dominik , Banach Marcin 
Published: November 2023

Abstract
This article addresses issues related to the use of nanoparticles as drug delivery carriers, with a particular focus on titanium dioxide (TiO2) nanoparticles. The aim of this study was to obtain TiO2 nanoparticles modified with glutathione in order to inhibit the release of titanium ions and reduce the toxic effects of TiO2 when used as a drug carrier. XRD analysis showed that some of the prepared samples had a crystalline structure, while others were amorphous. The size of crystallites was between 4.1 and 6.2 nm. The presence of glutathione in the structure of TiO2 particles was confirmed through attenuated total reflectance-Fourier transform infrared analysis (1,385 and 1,516 cm−1 for δ
N–H and ν
C═O stretching bands in NH3
+ and carboxylic groups). The size and stability of the nanoparticles assessed using the DLS technique revealed that the particles had a size range of 20–50 nm, and the electrokinetic potential of their suspensions was between −40.7 and −50.8 mV. The specific surface area, pore volume, and size were determined using nitrogen sorption. The measured specific surface area was equal to 230–390 m2/g. The amount of titanium ions released from the modified carriers was determined. It was lower by even over 70% compared to the not-modified sample. Also, the study involved the synthesis and characterisation of modified TiO2 nanoparticles loaded with tadalafil. It was found that the release of an active substance from the modified material was less, even 82% compared to the not-modified nanoparticles. Cytotoxic and mutagenic properties in relation to Chinese hamster ovary (CHO) cells were investigated. Titanium oxide nanoparticles modified with glutathione-enhanced CHO cell proliferation at over 60% compared to the reference material. Also, they had a less cytotoxic effect of over 37% compared to the reference material. The obtained materials show satisfactory purity and surface morphology, allowing the formation of carrier–drug interfaces. The results of in vitro studies let us claim that the prepared modified TiO2 nanoparticles have a great potential for being applied as a drug carrier.
Dynamical and physical characteristics of soliton solutions to the (2+1)-dimensional Konopelchenko–Dubrovsky system
Authors: Alruwaili Abdulmohsen D. , Seadawy Aly R. , Ali Asghar , Aldandani Mohammed M. 
Published: November 2023

Abstract
Soliton solutions of the Konopelchenko–Dubrovsky (KD) equation using four analytical methods are established. The KD system is used to study the portrays in physics with weak dispersion. The investigated results are obtained in different forms such as trigonometric, hyperbolic, and exponential functions. For the physical behavior of the concerned nonlinear system, some solutions are plotted graphically via assigning the certain values to the parameters. Mathematica software 11.11 is used to handle all results as well as figures. Hence, searched results have rewarding recompenses in nonlinear science.
TOLLIP acts as a cargo adaptor to promote lysosomal degradation of aberrant ER membrane proteins
Authors: Hayashi Yuki , Takatori Sho , Warsame Waleed Y , Tomita Taisuke , Fujisawa Takao , Ichijo Hidenori 
Published: November 2023

AbstractEndoplasmic reticulum (ER) proteostasis is maintained by various catabolic pathways. Lysosomes clear entire ER portions by ER‐phagy, while proteasomes selectively clear misfolded or surplus aberrant proteins by ER‐associated degradation (ERAD). Recently, lysosomes have also been implicated in the selective clearance of aberrant ER proteins, but the molecular basis remains unclear. Here, we show that the phosphatidylinositol‐3‐phosphate (PI3P)‐binding protein TOLLIP promotes selective lysosomal degradation of aberrant membrane proteins, including an artificial substrate and motoneuron disease‐causing mutants of VAPB and Seipin. These cargos are recognized by TOLLIP through its misfolding‐sensing intrinsically disordered region (IDR) and ubiquitin‐binding CUE domain. In contrast to ER‐phagy receptors, which clear both native and aberrant proteins by ER‐phagy, TOLLIP selectively clears aberrant cargos by coupling them with the PI3P‐dependent lysosomal trafficking without promoting bulk ER turnover. Moreover, TOLLIP depletion augments ER stress after ERAD inhibition, indicating that TOLLIP and ERAD cooperatively safeguard ER proteostasis. Our study identifies TOLLIP as a unique type of cargo‐specific adaptor dedicated to the clearance of aberrant ER cargos and provides insights into molecular mechanisms underlying lysosome‐mediated quality control of membrane proteins.
Combinatorial activation of the WNT‐dependent fibrogenic program by distinct complement subunits in dystrophic muscle
Authors: Florio Francesca , Vencato Sara , Papa Filomena T , Libergoli Michela , Kheir Eyemen , Ghzaiel Imen , Rando Thomas A , Torrente Yvan , Biressi Stefano 
Published: November 2023

AbstractFibrosis is associated with compromised muscle functionality in Duchenne muscular dystrophy (DMD). We report observations with tissues from dystrophic patients and mice supporting a model to explain fibrosis in DMD, which relies on the crosstalk between the complement and the WNT signaling pathways and the functional interactions of two cellular types. Fibro‐adipogenic progenitors and macrophages, which populate the inflamed dystrophic muscles, act as a combinatorial source of WNT activity by secreting distinct subunits of the C1 complement complex. The resulting aberrant activation of the WNT signaling in responsive cells, such as fibro‐adipogenic progenitors, contributes to fibrosis. Indeed, pharmacological inhibition of the C1r/s subunits in a murine model of DMD mitigated the activation of the WNT signaling pathway, reduced the fibrogenic characteristics of the fibro‐adipogenic progenitors, and ameliorated the dystrophic phenotype. These studies shed new light on the molecular and cellular mechanisms responsible for fibrosis in muscular dystrophy and open to new therapeutic strategies.
Pediatric Firearm Injury Emergency Department Visits From 2017 to 2022: A Multicenter Study
Authors: Hoffmann Jennifer A. , Carter Camille P. , Olsen Cody S. , Chaudhari Pradip P. , Chaudhary Sofia , Duffy Susan , Glomb Nicolaus , Goyal Monika K. , Grupp-Phelan Jacqueline , Haasz Maya , Ketabchi Bijan , Kravitz-Wirtz Nicole , Lerner E. Brooke , Shihabuddin Bashar , Wendt Wendi , Cook Lawrence J. , Alpern Elizabeth R. ,   PECARN Registry Study Group
Published: November 2023

BACKGROUND AND OBJECTIVE
Pediatric firearm injuries increased during the coronavirus disease 2019 pandemic, but recent trends in firearm injury emergency department (ED) visits are not well described. We aimed to assess how pediatric firearm injury ED visits during the pandemic differed from expected prepandemic trends.

METHODS
We retrospectively studied firearm injury ED visits by children &lt;18 years old at 9 US hospitals participating in the Pediatric Emergency Care Applied Research Network Registry before (January 2017 to February 2020) and during (March 2020 to November 2022) the pandemic. Multivariable Poisson regression models estimated expected visit rates from prepandemic data. We calculated rate ratios (RRs) of observed to expected visits per 30 days, overall, and by sociodemographic characteristics.

RESULTS
We identified 1904 firearm injury ED visits (52.3% 15–17 years old, 80.0% male, 63.5% non-Hispanic Black), with 694 prepandemic visits and 1210 visits during the pandemic. Death in the ED/hospital increased from 3.1% prepandemic to 6.1% during the pandemic (P = .007). Firearm injury visits per 30 days increased from 18.0 prepandemic to 36.1 during the pandemic (RR 2.09, 95% CI 1.63–2.91). Increases beyond expected rates were seen for 10- to 14-year-olds (RR 2.61, 95% CI 1.69–5.71), females (RR 2.46, 95% CI 1.55–6.00), males (RR 2.00, 95% CI 1.53–2.86), Hispanic children (RR 2.30, 95% CI 1.30–9.91), and Black non-Hispanic children (RR 1.88, 95% CI 1.34–3.10).

CONCLUSIONS
Firearm injury ED visits for children increased beyond expected prepandemic trends, with greater increases among certain population subgroups. These findings may inform firearm injury prevention efforts.
<I>Richardia Scabra</I> L. (Rubiaceae): A New Angiospermic Record For The Flora Of Western Himalaya, India
Authors: Ahmed Mushtaq , Dhiman Manjul , Singh Bagri Ajendra 
Published: November 2023

In present study Richardia scabra L. was found as a new distribution record to the state Jammu and Kashmir, Western Himalaya, India.Â R. scabra L. is a terrestrial herb commonly known as â€˜Rough Mexican cloverâ€™, also called â€˜Florida pusleyâ€™ belongs to the family Rubiaceae.
Entorhinal cortex vulnerability to human APP expression promotes
hyperexcitability and tau pathology
Authors: Rowan Matthew , Goettemoeller Annie , Banks Emmie , McCann Katharine , Kumar Prateek , South Kelly , Olah Viktor , Ramelow Christina , Duong Duc , Seyfried Nicholas , Rangaraju Srikant , Weinshenker David 
Published: November 2023

Abstract

Preventative treatment for Alzheimer’s Disease is of dire importance, and yet, cellular mechanisms underlying early regional vulnerability in Alzheimer’s Disease remain unknown. In human patients with Alzheimer’s Disease, one of the earliest observed pathophysiological correlates to cognitive decline is hyperexcitability1. In mouse models, early hyperexcitability has been shown in the entorhinal cortex, the first cortical region impacted by Alzheimer’s Disease2-4. The origin of hyperexcitability in early-stage disease and why it preferentially emerges in specific regions is unclear. Using cortical-region and cell-type- specific proteomics and patch-clamp electrophysiology, we uncovered differential susceptibility to human-specific amyloid precursor protein (hAPP) in a model of sporadic Alzheimer’s. Unexpectedly, our findings reveal that early entorhinal hyperexcitability may result from intrinsic vulnerability of parvalbumin interneurons, rather than the suspected layer II excitatory neurons. This vulnerability of entorhinal PV interneurons is specific to hAPP, as it could not be recapitulated with increased murine APP expression. Furthermore, the Somatosensory Cortex showed no such vulnerability to adult-onset hAPP expression, likely resulting from PV-interneuron variability between the two regions based on physiological and proteomic evaluations. Interestingly, entorhinal hAPP-induced hyperexcitability was quelled by co-expression of human Tau at the expense of increased pathological tau species. This study suggests early disease interventions targeting non-excitatory cell types may protect regions with early vulnerability to pathological symptoms of Alzheimer’s Disease and downstream cognitive decline.
Solution-processed NO2 gas sensor based on PbS QDs doped with P3HT operating at room temperature
Authors: Kwon JinBeom , Jung Daewoong , Ha Yuntae , Choe Suji , Jung Donggeon 
Published: November 2023

Abstract

With the worldwide industrial development and the increase in means of transportation such as automobiles and ships, the problem of the greenhouse gases has been steadily emerging. Among them, NO2 is a gas that is emitted in large quantities from the automobile and factories, and is unavoidably emitted in modern society. Therefore, a sensor capable of precisely detecting NO2 is required. Among various types of NO2 sensors, metal oxide-based sensors are reported the most. However, it is impossible to operate at room temperature due to its high-temperature operating characteristics, so a heater for heating must be designed inside the sensor or installed outside. On the other hand, the NO2 sensor based on PbS QDs has the advantage of being able to measure at room temperature and being easily manufactured through a solution process rather than a complicated semiconductor process. In this study, NO2 sensor was fabricated by doping P3HT on PbS QDs. As a result of the responsivity of the fabricated sensor to NO2 gas, it was confirmed that the sensor had high responsivity with a resolution of 200 ppb from 20 to 0.4 ppm due to the stability of the thin film and high hole mobility characteristics by P3HT.
Endocannabinoids and their receptors modulate endometriosis pathogenesis and immune response
Authors: Tayade Chandrakant , Lingegowda Harshavardhan , Zutautas Katherine , Wei Yuhong , Yolmo Priyanka , Sisnett Danielle , McCallion Alison , Koti Madhuri 
Published: November 2023

Abstract

Endometriosis (EM), characterized by the presence of endometrial-like tissue outside the uterus, is the leading cause of chronic pelvic pain and infertility in females of reproductive age. Despite its high prevalence, the molecular mechanisms underlying EM pathogenesis remain poorly understood. The endocannabinoid system (ECS) is known to influence several cardinal features of this complex disease including pain, vascularization, and overall lesion survival, but the exact mechanisms are not known. Utilizing CNR1 knockout (k/o), CNR2 k/o, and wild-type (WT) mouse models of EM, we reveal the contributions of ECS and these receptors in disease initiation, progression, and immune modulation. Particularly, we identified EM-specific T cell dysfunction in the CNR2 k/o mouse model of EM. We also demonstrate the impact of decidualization-induced changes on ECS components and the unique disease-associated transcriptional landscape of ECS components in EM. Imaging Mass Cytometry (IMC) analysis revealed distinct features of the microenvironment between CNR1, CNR2, and WT genotypes in the presence or absence of decidualization. This study, for the first time, provides an in-depth analysis of the involvement of the ECS in EM pathogenesis and lays the foundation for the development of novel therapeutic interventions to alleviate the burden of this debilitating condition.
In-depth Inference of Transcriptional Regulatory Networks Identifies NPM1 as a Druggable Master Regulator in Medulloblastoma
Authors: Lin Yifeng , Dong Xinran , Chen Tong , Chen Huiyao , Xia Mingyang , Liao Yunfei , Li Hao , Zhou Wenhao 
Published: November 2023

Abstract

Medulloblastoma (MB) is a highly malignant embryonic brain tumor with distinct cytogenetic heterogeneity in children. Despite elucidating divergent regulatory underpinnings in MB molecular subtypes, the hidden oncogenic signaling drivers with minimal differential expression remain obscure. Here, we leveraged two bulk RNA-seq datasets involving 1055 patients to construct pediatric MB specific co-expression profiles and regulatory networks, unveiling unique attributes of the ribosome pathway in different molecular subtypes. NPM1, a pivotal ribosome regulator, was pinpointed as a promising targetable driver for MB by integrating differentially expressed candidates and reported drug targets. To assess the role of NPM1 in MB progression, we employed NETBID2 gene activity algorithm, Kaplan–Meier survival curves, single-cell RNA-seq (scRNA-seq) analysis, and functional validations in MB cell lines. Our findings highlighted substantial correlations between the activity values of NPM1 and the prognosis of MB patients, eclipsing the significance of its expression levels. Furthermore, NPM1 was extensively and highly expressed in early SOX2 positive neural stem cells and tumor cells. Deficiency of NPM1 impeded MB cell growth, triggered apoptosis and led to a reduction of c-Myc levels. Our findings provide new approaches for exploring underlying pathways and underscore the clinical transformation value of NPM1 as a master regulator for MB.
A taxonomy of patient experiences of access and continuity at team-based primary healthcare clinics
Authors: Deville-Stoetzel Nadia , Gaboury Isabelle , Berbiche Djamal , Breton Mylaine 
Published: November 2023

Abstract

Background
Access to primary healthcare services is a core lever for reducing health inequalities. Population groups living with certain individual social characteristics are disproportionately more likely to experience barriers accessing care. This study identified profiles of access and continuity experiences of patients registered at team-based primary healthcare clinics and explored the associations of these profiles with individual and organizational characteristics.
Methods
A cross-sectional e-survey was conducted between September 2022 and April 2023. All registered adult patients with an email address at 104 team-based primary healthcare clinics in Quebec were invited to participate. Latent class analysis was used to identify patient profiles based on nine components of access to care and continuity experiences. Multinomial logistic regression models were fit to analyze each profile’s association with ten characteristics related to individual sociodemographics and two related to clinic area and size.
Results
Based on 87,155 patient experiences, four profiles were identified. "Easy access and continuity" (42% of respondents) was characterized by ease in almost all access and continuity components. Three profiles were characterized by diverging access and/or continuity difficulties. "Challenging booking" (32%) was characterized by patients having to try several times to obtain an appointment at their clinic. "Challenging continuity" (9%) was characterized by patients having to repeat information that should have been in their file. "Access and continuity barriers" (16%) was characterized by difficulties with all access and continuity components. Female gender and poor perceived health significantly increased the risk of belonging to the three profiles associated with difficulties by 1.5. Being a recently arrived immigrant (p = 0.036), having less than a high school education (p = 0.002) and being registered at a large clinic (p < 0.001) were associated with experiencing booking difficulties. Having at least one chronic condition (p = 0.004) or poor perceived mental health (p = 0.048) were associated with experiencing continuity difficulties.
Conclusions
These results highlight individual social characteristics associated with increased risk of experiencing particular access and/or continuity difficulties. Personalized strategies are recommended, such as facilitating appointment booking for recently arrived immigrants and patients with low education, integrating interprofessional collaboration practices for patients with chronic conditions and improving care coordination and communication for patients with mental health needs.