bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and Rationale of Endoscopic Ultrasound–Guided Thrombin Injection in Visceral Artery Pseudoaneurysm Not Amenable to Angioembolization
Authors: Jhajharia Ashok , Singh Shashank , Chaudhary Monika , Ameta Mayank , Ashdhir Prachis , Nijhawan Sandeep 
Published: February 2024

Background
Pseudoaneurysm usually occurs after vascular injuries or erosions such as in trauma or inflammation like pancreatitis and is associated with high morbidity and mortality.

Aim
The aim of study is to assess efficacy and safety of EUS-guided thrombin injection in pseudoaneurysm.

Materials and Methods
Prospective data collection was done at SMS Hospital, Jaipur, from January 2015 to March 2023. All patients with pseudoaneurysm were consecutively enrolled.

Results
Twenty patients (M/F, 18:2) with median age of 41 years (25–58 years), were studied. Underlying etiology of pseudoaneurysm was chronic pancreatitis in 75% of the patients, blunt trauma abdomen in 15% of the patients, recurrent acute pancreatitis in 5%, and idiopathic in 5% of the patients. At the time of admission, mean hemoglobin was 6.7 g/dL (3.4–8.2), with median blood transfusion requirement was 2 units (0–6 units). Hemoglobin values after 4–6 weeks showed a significant improvement (t = 9.21, P < 0.05).
Mean dose of human thrombin required for complete obliteration of pseudoaneurysm was 520 ± 188.6 IU per patient (300–800 IU). Amount of thrombin (IU) dose needed to achieve complete obliteration correlated well significantly with the dimension of pseudoaneurysm, P value less than 0.05 (R = 0.80). Median follow-up duration in this study was 44 months (3–84 months), which was the longest follow-up period by far.

Conclusions
Endoscopic ultrasound–guided thrombin injection in visceral artery pseudoaneurysm is a safe and effective alternative for patients not amenable for digital subtraction angiography–guided angioembolization.
The epidemiology of neck and low back pain in Iran: a national and sub-national analysis from 1990 to 2019
Authors: Khadembashiri Mohamad M. , Khadembashiri Mohamad A. , Khonji Mohammad S. , Ahadi Tannaz , Forogh Bijan , Mirdamadi Niloofar , Ahmadi Mohammad , Bagherzadeh Cham Masumeh , Soleymanzadeh Hosna , Raissi Gholamreza 
Published: February 2024

Background:
Neck pain (NP) is a condition influenced by multiple factors. It places a significant burden on individuals suffering from NP and on social and economic systems. On a global scale, low back pain (LBP) stands out as a significant contributor to years lost to disability, and this burden is on the rise due to population growth and aging.

Methods:
The Global Burden of Disease database was used to collect data on the prevalence, incidence, and years lived with disability (YLD) of NP and LBP between 1990 and 2019. Various factors, including age group, gender, Iran, and its 31 provinces, were used to classify the data.

Results:
Iran accounted for 0.86 million incident cases of NP in 2019, with age-standardized incident rate per 100 000 population of 934.1. Tehran has the maximum age-standardized prevalence, incidence, and YLD. Iran accounted for the age-standardized incidence rate for LBP per 100 000 population of 3492.9, and it reduced to −8.35% from 1990. Mazandaran exhibits the highest levels of prevalence, incidence, and YLD for LBP in 2019. In Iran, the point prevalence of NP in 2019 was higher in females and increased with age up to 50–54 years for females and 70–74 years for males. However, there is only a slight difference between females and males in Iran regarding LBP prevalence.

Conclusions:
In this study, the authors report the prevalence, incidence, YLD, and age-standardized rates for NP and LBP in the world’s general population and Iran’s population based on its sub-nations in 1990 and 2019.
A digital health intervention to support patients with chronic pain during prescription opioid tapering: a pilot randomised controlled trial
Authors: Gholamrezaei Ali , Magee Michael R. , McNeilage Amy G. , Dwyer Leah , Sim Alison , Ferreira Manuela L. , Darnall Beth D. , Brake Timothy , Aggarwal Arun , Craigie Meredith , Hollington Irina , Glare Paul , Ashton-James Claire E. 
Published: February 2024

Abstract

Introduction:
Recent changes in opioid prescribing guidelines have led to an increasing number of patients with chronic pain being recommended to taper. However, opioid tapering can be challenging, and many patients require support.

Objectives:
We evaluated the feasibility, acceptability, and potential efficacy of a codesigned digital health intervention to support patients with chronic pain during voluntary prescription opioid tapering.

Methods:
In a pilot randomised controlled trial, participants received a psychoeducational video and 28 days of text messages (2 SMS/day) in addition to their usual care (intervention) or usual care alone (control). The feasibility, acceptability, and potential efficacy of the intervention were evaluated. The primary outcome was opioid tapering self-efficacy. Secondary outcomes were pain intensity and interference, anxiety and depression symptom severity, pain catastrophising, and pain self-efficacy.

Results:
Of 28 randomised participants, 26 completed the study (13 per group). Text message delivery was high (99.2%), but fidelity of video delivery was low (57.1%). Most participants rated the messages as useful, supportive, encouraging, and engaging; 78.5% would recommend the intervention to others; and 64.2% desired a longer intervention period. Tapering self-efficacy (Cohen d = 0.74) and pain self-efficacy (d = 0.41) were higher, and pain intensity (d = 0.65) and affective interference (d = 0.45) were lower in the intervention group at week 4.

Conclusion:
First evidence supports the feasibility, acceptability, and potentially efficacy of a psychoeducational video and SMS text messaging intervention to support patients with chronic pain during voluntary prescription opioid tapering. Definitive trials with longer intervention duration are warranted.
Multi-Institution Survey of Accepting Physicians’ Perception of Appropriate Reasons for Interhospital Transfer: A Mixed-Methods Evaluation
Authors: Nguyen Khanh T. , Lee Tiffany M. , Mueller Stephanie K. 
Published: February 2024

Objectives
There is a lack of evidence-based guidelines to direct best practices in interhospital transfers (IHTs). We aimed to identify frontline physicians’ current and ideal reasons for accepting IHT patients to inform future IHT research and guidelines.

Methods
We conducted a cross-sectional survey of hospitalist physicians across 11 geographically diverse hospitals. The survey asked respondents how frequently they currently consider and should consider various factors when triaging IHT requests. Responses were dichotomized into “highly considered” and “less considered” factors. Frequencies of the “highly considered” factors (current and ideal) were analyzed. Write-in responses were coded into themes within a priori domains in a qualitative analysis.

Results
Of the 666 hospitalists surveyed, 238 (36%) responded. Respondents most frequently identified the need for specialty procedural and nonprocedural care and bed capacity as factors that should be considered when triaging IHT patients in current and ideal practice, whereas the least frequently considered factors were COVID-related care, insurance/financial considerations, and patient/family preference. More experienced respondents considered patient/family preference more frequently in current and ideal practice compared with less experienced respondents (33% versus 11% [P = 0.0001] and 26% versus 9% [P = 0.01], respectively). Qualitative analysis identified several themes in the domains of Criteria for Acceptance, Threshold for Acceptance, and Indications for Physician-to-Physician Communication.

Conclusions
This geographically diverse sample of hospitalist physicians responsible for accepting IHT patients showed general agreement between primary factors that are currently and that should be considered for IHT acceptance, with greatest weight placed on patients’ need for specialty care.
Using Bibliometric Analysis to Map the Impact of a Simulation Program's Published Scholarly Works
Authors: Rodgers David L. , Bohrer Maxwell B. , Morales Adrian A. , Needler Matthew D. , VandeKoppel Paul 
Published: February 2024

Summary Statement
Bibliometrics quantitatively evaluates the targeted literature sources and can help define research and scholarly publications' impact and demonstrate connections for authors, departments, or universities. This article presents a methodology for simulation programs to evaluate their influence in terms of both impact and scope of their published simulation-based healthcare scholarly output. Using the authors' home university and healthcare system as an example, the article outlines a methodology to map research and scholarly works networks within the systems, identify and map connections outside the system, and quantifiably score the overall impact of the simulation program's scholarly output using a common scoring metric, the h-index. This generates an objective measure of impact, rather than a subjective opinion of an organization's research and scholarly impact. The combination of an institutional h-index with mapping of simulation-based healthcare scholarly output provides a full, objective description of the institution's output and provides a benchmark for other simulation programs for comparison.
Linking geodiversity and geosystem services to human well-being for the sustainable utilization of the subsurface and the urban environment
Authors: van Ree D. , van Beukering P. J. H. , Hofkes M. W. 
Published: February 2024

Because the functions of the subsurface are hidden from view, its important role in society is often ignored or taken for granted. The subsurface is, however, an essential part of the global ecosystem with important contributions to human well-being. Geodiversity is an important characteristic in this respect. Material supply is the more obvious role of the subsurface with projections of a doubling of global material use in 2060 as compared to 2017. Moreover, creating underground spaces and infrastructure are gaining importance in the urban environment. The main reason for the inadequate protection of geodiversity is the lack of a comprehensive and integrative framework. Linking socio-economic activities to biophysical system characteristics of the subsurface is facilitated by the geosystem services approach. Sustainable urban development strategies require including geodiversity in decision-making on human well-being and setting conditions for land use change. Spatial plans and decisions on the use of natural endowments should look at processes over much longer timeframes. In this paper, we explore the links between human well-being and the subsurface with an emphasis on the role of geodiversity. We set out a methodological framework and describe possible long term three-dimensional land use planning consequences for sustainable utilization of the subsurface.
This article is part of the Theo Murphy meeting issue ‘Geodiversity for science and society’.
The influence of scale-dependent geodiversity on species distribution models in a biodiversity hotspot
Authors: Gerstner Beth E. , Blair Mary E. , Bills Patrick , Cruz-Rodriguez Cristian A. , Zarnetske Phoebe L. 
Published: February 2024

Improving models of species' distributions is essential for conservation, especially in light of global change. Species distribution models (SDMs) often rely on mean environmental conditions, yet species distributions are also a function of environmental heterogeneity and filtering acting at multiple spatial scales. Geodiversity, which we define as the variation of abiotic features and processes of Earth's entire geosphere (inclusive of climate), has potential to improve SDMs and conservation assessments, as they capture multiple abiotic dimensions of species niches, however they have not been sufficiently tested in SDMs. We tested a range of geodiversity variables computed at varying scales using climate and elevation data. We compared predictive performance of MaxEnt SDMs generated using CHELSA bioclimatic variables to those also including geodiversity variables for 31 mammalian species in Colombia. Results show the spatial grain of geodiversity variables affects SDM performance. Some variables consistently exhibited an increasing or decreasing trend in variable importance with spatial grain, showing slight scale-dependence and indicating that some geodiversity variables are more relevant at particular scales for some species. Incorporating geodiversity variables into SDMs, and doing so at the appropriate spatial scales, enhances the ability to model species-environment relationships, thereby contributing to the conservation and management of biodiversity.
This article is part of the Theo Murphy meeting issue ‘Geodiversity for science and society’.
Promoting diversity in geoheritage evaluation: creating an evaluation method for the scientific value of Quaternary sites in arid environments
Authors: Sayama Kenta 
Published: February 2024

A rigorous assessment is an essential part of geoconservation, and choosing an appropriate evaluation method is essential for this process. Globally, an increasing number of sites are being assessed for their geoheritage values, but the most popular methods have been created by researchers with experiences centred in Europe, without considerations of regional differences. To understand whether regional perspectives are required in the evaluation process, this study developed a new method targeting the scientific value of Quaternary geoheritage sites in arid environments, using input from 49 researchers in geosciences and related disciplines with interests in arid landscapes. The results demonstrated the need for a new or modified method, given the different preferences in the weighting system and the necessity to include additional criteria specific to the type of sites targeted by the study. The strong preference to include a new criterion on connections with archaeology/anthropology highlighted the significance of the interdisciplinary scientific values of Quaternary geoheritage sites in arid environments. These findings imply the need for regional diversity or adjustments in geoheritage evaluation. Future research is required to consider such differences for geoheritage values beyond the scientific dimension, such as educational values and touristic values in diverse geographical settings.
This article is part of the Theo Murphy meeting issue 'Geodiversity for science and society'.
Speluncibacter jeojiensis gen. nov. sp. nov., a novel bacterium of the order Mycobacteriales isolated from a cave and a proposal of Speluncibacteraceae fam. nov.
Authors: Lee Soon Dong , Yang Hong-Lim , Han Jong-Heon , Kim In Seop 
Published: February 2024

Two Gram-stain-positive, aerobic, non-spore-forming, non-motile, irregular rod-shaped actinobacteria, designated as D2-41T and D3-21, were isolated from soil samples collected in a natural cave in Jeju, Republic of Korea. Both of the isolates were shown to share 100 % 16S rRNA sequence identity. The cell wall contained meso-diaminopimelic acid, arabinose and galactose. The predominant menaquinone was MK-8(H2). The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified aminolipid, an unidentified aminoglycolipid, an unidentified phospholipid and two unidentified lipids. The predominant fatty acids were C16 : 0 and summed feature 3 (C16 : 1
ω7c and/or iso-C15 : 0 2-OH). Mycolic acids of C30–C38 were present. The 16S rRNA gene trees showed that the organisms occupied a distinct position remotely located from recognized genera within the order Mycobacteriales, albeit with the 16S rRNA gene similarities of 97.0–97.1 % with Rhodococcus olei, Rhodococcus rhodnii and Rhodococcus triatomae. The genome sizes and DNA G+C contents of strains D2-41T and D3-21 were 4.77–4.88 Mbp and 69.8 mol%, respectively. Both of the isolates shared an average nucleotide identity of 99.4 % and digital DNA–DNA hybridization of 95.2 % to each other, revealing that strains D2-41T and D3-21 belonged to the same species. In the core genome-based phylogenomic tree, both of the isolates were found to be closely associated with members of the genus Tomitella. However, strains D2-41T and D3-21 revealed the highest amino acid identity values (mean 66.5 %, range 66.2–67.0 % with the genus Prescottella of the family Nocardiaceae, followed by the genus Tomitella (mean 64.1 %, range 63.6–64.7 %) of the family Tomitellaceae. Based on the combined data obtained here, the novel isolates belong to a new genus of the new family for which the name Speluncibacter jeojiensis gen. nov. sp. nov. is proposed, with Speluncibacteraceae fam. nov. The type strain is strain D2-41T (=KACC 17930T=DSM 101875T).
Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing
Authors: Fiflis David N. , Rey Nicolas A. , Venugopal-Lavanya Harshitha , Sewell Beatrice , Mitchell-Dick Aaron , Clements Katie N. , Milo Sydney , Benkert Abigail R. , Rosales Alan , Fergione Sofia , Asokan Aravind 
Published: February 2024

AbstractType VI CRISPR enzymes have been developed as programmable RNA-guided Cas proteins for eukaryotic RNA editing. Notably, Cas13 has been utilized for site-targeted single base edits, demethylation, RNA cleavage or knockdown and alternative splicing. However, the ability to edit large stretches of mRNA transcripts remains a significant challenge. Here, we demonstrate that CRISPR-Cas13 systems can be repurposed to assist trans-splicing of exogenous RNA fragments into an endogenous pre-mRNA transcript, a method termedCRISPRAssisted mRNAFragmentTrans-splicing (CRAFT). Using split reporter-based assays, we evaluate orthogonal Cas13 systems, optimize guide RNA length and screen for optimal trans-splicing site(s) across a range of intronic targets. We achieve markedly improved editing of large 5’ and 3’ segments in different endogenous mRNAs across various mammalian cell types compared to other spliceosome-mediated trans-splicing methods. CRAFT can serve as a versatile platform for attachment of protein tags, studying the impact of multiple mutations/single nucleotide polymorphisms, modification of untranslated regions (UTRs) or replacing large segments of mRNA transcripts.
Morphologic characterization and cytokine response of chicken bone-marrow derived dendritic cells to infection with high and low pathogenic avian influenza virus
Authors: Mo Jongsuk , Segovia Karen , Chrzastek Klaudia , Briggs Kelsey , Kapczynski Darrell R. 
Published: February 2024

AbstractDendritic cells (DCs) are professional antigen-presenting cells, which are key components of the immune system and involved in the early immune response. DCs are specialized in capturing, processing, and presenting antigens to facilitate immune interactions. Chickens infected with avian influenza virus (AIV) demonstrate a wide range of clinical symptoms, based on pathogenicity of the virus. Low pathogenic avian influenza (LPAI) viruses typically induce mild clinical signs, whereas high pathogenic avian influenza (HPAI) induce more severe disease, which can lead to death within days. For this study, chicken bone marrow-derived DC (ckBM-DC)s were produced and infected with high and low pathogenic avian influenza viruses of H5N2 or H7N3 subtypes to characterize innate immune responses, study effect on cell morphology, and evaluate virus replication. A strong proinflammatory response, including chicken interleukin-1β, and stimulation of the interferon response pathway were observed at 8 hours post infection. Microscopically, the DCs underwent morphological changes from classic elongated dendrites to a more general rounded shape that eventually lead to cell death with the presence of scattered cellular debris. Differences in onset of morphologic changes were observed between H5 and H7 subtypes. Increases in viral titers demonstrated that both HPAI and LPAI are capable of infecting and replicating in DCs. The elevated expression of infected DCs may be indicative with a dysregulation of the immune response typically seen with HPAI infections.
Single-Cell Transcriptomic Profiling Unveils Critical Metabolic Alterations and Signatures in Progression of Atherosclerosis
Authors: Ma Limei , Zhao Ying , Xiang Peng , Chen Qingqiu , Hassan Moustapha , Yu Chao 
Published: February 2024

AbstractMetabolic dysregulation is recognized as a fundamental characteristic of cardiovascular diseases (CVDs), therefore mining metabolic patterns in these diseases would help to identify the possible pathogenic mechanisms and potential intervention targets. Atherosclerosis (AS), serving as the foundational pathology in numerous CVDs, represents a paramount global health concern. However, a systematic integrated analysis of the metabolic networks of AS is still lacking. In this study, we investigated and integrated single-cell RNA sequencing datasets from calcified atherosclerotic core (AC) plaques and patient-matched proximal adjacent (PA) portions of carotid artery tissue to generate metabolic flux profiling at single-cell level. Using scFEA and scWGCNA analyses, we discerned common metabolic changes in endothelial cells (ECs), myeloid cells, and smooth muscle cells. These altered metabolic modules were predominantly enriched in glucose-related pathways and were predicted to potentially facilitate metabolic bypass. Of particular interest, we observed an enrichment of metabolites produced from glucose/sialic acid metabolism pathway in both ECs and myeloid cells. This observation may partially account for their positive involvement in plaque formation, as previously discovered. Furthermore, we predicted that metabolic genes such asHK1,ENO1,PFKL,LDHA,PGK1,andNANSmay be implicated in the detected metabolic flux disorder during AS progression. Additionally, we uncovered interactions between various cell types at single-cell level using CellChat. We noted heightened interactions between endothelial/SMC as well as myeloid/ECs in AC group, with ITGB2/VCAM and CD44/CD74 receptors potentially participating in these interactions, thereby fostering the development of pro-inflammatory plaque microenvironment. In conclusion, our study unveils metabolic shifts at single-cell level and identifies key gene signatures associated with metabolic disorders and cell-cell communication in atherosclerosis.