bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐Atom Zinc Sites with Synergetic Multiple Coordination Shells for Electrochemical H2O2 Production
Authors: Wei Gangya , Li Yunxiang , Liu Xupo , Huang Jinrui , Liu Mengran , Luan Deyan , Gao Shuyan , Lou Xiong Wen (David) 
Published: October 2023

AbstractPrecise manipulation of the coordination environment of single‐atom catalysts (SACs), particularly the simultaneous engineering of multiple coordination shells, is crucial to maximize their catalytic performance but remains challenging. Herein, we present a general two‐step strategy to fabricate a series of hollow carbon‐based SACs featuring asymmetric Zn−N2O2 moieties simultaneously modulated with S atoms in higher coordination shells of Zn centers (n≥2; designated as Zn−N2O2−S). Systematic analyses demonstrate that the synergetic effects between the N2O2 species in the first coordination shell and the S atoms in higher coordination shells lead to robust discrete Zn sites with the optimal electronic structure for selective O2 reduction to H2O2. Remarkably, the Zn−N2O2 moiety with S atoms in the second coordination shell possesses a nearly ideal Gibbs free energy for the key OOH* intermediate, which favors the formation and desorption of OOH* on Zn sites for H2O2 generation. Consequently, the Zn−N2O2−S SAC exhibits impressive electrochemical H2O2 production performance with high selectivity of 96 %. Even at a high current density of 80 mA cm−2 in the flow cell, it shows a high H2O2 production rate of 6.924 mol gcat−1 h−1 with an average Faradaic efficiency of 93.1 %, and excellent durability over 65 h.
An Fr 13 risk analysis of failure in a global food process – illustration with milk processing
Authors: Chandrakash Saravanan , Davey Kenneth , O'Neill Brian 
Published: October 2023

AbstractA novel Friday 13th (Fr 13) risk analysis of the vulnerability to surprise (unexpected) failure of a global milk pasteurizing plant consisting of heating, holding and cooling unit operations is developed and illustrated. We define two or more interconnected unit operations as a global model. The aim was to gain new insight into the effects of accumulating random (stochastic) changes that could lead to overall failure in an otherwise well‐operated and well‐maintained pasteurizing plant. The methodology is a development of the work of Davey and co‐workers (Food Control 29, 1 (2015) 248–253, CES 127 (2015) 133–142). Failure is defined in terms of criteria that must be met to ensure safe operation. Realistic data for large‐scale pasteurization are used. Results reveal that the overall global model is vulnerable to failure in 12.5% of all cases over the long term. If each simulation can be considered a daily process, this translates to some 46 failures each year to meet the design pasteurization criteria. This new process insight cannot be obtained using traditional risk and hazard approaches. How this new Fr 13 methodology can be used to simulate design changes to the physical system to minimize vulnerability to failure is discussed. The finding should be of immediate interest to milk processors. © 2015 Curtin University of Technology and John Wiley & Sons, Ltd.
α‐Ketoglutarate–Dependent KDM6 Histone Demethylases and Interferon‐Stimulated Gene Expression in Lupus
Authors: Montano Erica N. , Bose Moumita , Huo Lihong , Tumurkhuu Gantsetseg , De Los Santos Gabriela , Simental Brianna , Stotland Aleksandr B. , Wei Janet , Bairey Merz C. Noel , Suda Jo , Martins Gislaine , Lalani Sarfaraz , Lawrenson Kate , Wang Yizhou , Parker Sarah , Venuturupalli Swamy , Ishimori Mariko , Wallace Daniel J. , Jefferies Caroline A. 
Published: October 2023

ObjectiveWe aimed to investigate the hypothesis that interferon (IFN)–stimulated gene (ISG) expression in systemic lupus erythematosus (SLE) monocytes is linked to changes in metabolic reprogramming and epigenetic regulation of ISG expression.MethodsMonocytes from healthy volunteers and patients with SLE at baseline or following IFNα treatment were analyzed by extracellular flux analysis, proteomics, metabolomics, chromatin immunoprecipitation, and gene expression. The histone demethylases KDM6A/B were inhibited using glycogen synthase kinase J4 (GSK‐J4). GSK‐J4 was tested in pristane and resiquimod (R848) models of IFN‐driven SLE.ResultsSLE monocytes had enhanced rates of glycolysis and oxidative phosphorylation compared to healthy control monocytes, as well as increased levels of isocitrate dehydrogenase and its product, α‐ketoglutarate (α‐KG). Because α‐KG is a required cofactor for histone demethylases KDM6A and KDM6B, we hypothesized that IFNα may be driving “trained immune” responses through altering histone methylation. IFNα priming (day 1) resulted in a sustained increase in the expression of ISGs in primed cells (day 5) and enhanced expression on restimulation with IFNα. Importantly, decreased H3K27 trimethylation was observed at the promoters of ISGs following IFNα priming. Finally, GSK‐J4 (KDM6A/B inhibitor) resulted in decreased ISG expression in SLE patient monocytes, as well as reduced autoantibody production, ISG expression, and kidney pathology in R848‐treated BALB/c mice.ConclusionOur study suggests long‐term IFNα exposure alters the epigenetic regulation of ISG expression in SLE monocytes via changes in immunometabolism, a mechanism reflecting trained immunity to type I IFN. Importantly, it opens the possibility that targeting histone‐modifying enzymes, such as KDM6A/B, may reduce IFN responses in SLE.image
Shortened time to diagnosis for patients suspected of urinary bladder cancer managed in a standardized care pathway was associated with an improvement in tumour characteristics
Authors: Abuhasanein Suleiman , Jahnson Staffan , Kjölhede Henrik 
Published: October 2023

AbstractObjectivesTo evaluate whether the implementation of standardized care pathway (SCP) for patients with suspected urinary bladder cancer (UBC) was associated with changes in tumour characteristics. Additionally, the study aims to explore whether there was a shift in the selection of patients prioritized for immediate evaluation regarding suspicion of UBC.Materials and MethodsThe study included all patients diagnosed with UBC in the NU Hospital Group between 2010 and 2019. To evaluate changes associated with SCP, patients were divided into two diagnostic time periods, either before (2010–2015) or during (2016–2019) the implementation of the SCP. To evaluate which patients were prioritized for prompt evaluation within 13 days, logistic regression analysis was performed on all patients before and during SCP.ResultsMedian time to transurethral resection of the tumour in urinary bladder (TURBT) decreased from 29 days (interquartile range [IQR] 16–48) before SCP to 12 days (IQR 8–19) during SCP (p < 0.001) with a clear break from 2016. The proportion of cT2 + tumours decreased during SCP from 26% to 20% (p = 0.035). In addition, tumours detected during SCP were smaller (p = 0.023), but with more multiple lesions (p = 0.055) and G3 tumours (p = 0.007). During SCP, there was no statistically significant difference between the groups of patients with TURBT within or after 13 days. In contrast, before SCP, a majority of the patients treated within 13 days had advanced tumours and were admitted from the emergency ward.ConclusionsThe implementation of an SCP for suspected UBC was associated with improved tumour characteristics. Interestingly, during SCP, there were no substantial differences in patients' or tumours' characteristics among those who underwent TURBT within or after 13 days. This indicates that the 13‐day timeframe for TURBT might be prolonged, especially in less urgent cases in order to facilitate a prioritization of more severe cases with treatable disease.
Proportion of collagen type II in the extracellular matrix promotes the differentiation of human adipose‐derived mesenchymal stem cells into nucleus pulposus cells
Authors: Tao Yiqing , Zhou Xiaopeng , Liu Dongyu , Li Hao , Liang Chengzhen , Li Fangcai , Chen Qixin 
Published: October 2023

AbstractDuring degeneration process, the catabolism of collagen type II and anabolism of collagen type I in nucleus pulposus (NP) may influence the bioactivity of transplanted cells. Human adipose‐derived mesenchymal stem cells (hADMSCs) were cultured as a micromass or in a series of gradual proportion hydrogels of a mix of collagen types I and II. Cell proliferation and cytotoxicity were detected using CCK‐8 and LDH assays respectively. The expression of differentiation‐related genes and proteins, including SOX9, aggrecan, collagen type I, and collagen type II, was examined using RT‐qPCR and Western blotting. Novel phenotypic genes were also detected by RT‐qPCR and western blotting. Alcian blue and dimethylmethylene blue assays were used to investigate sulfate proteoglycan expression, and PI3K/AKT, MAPK/ERK, and Smad signaling pathways were examined by Western blotting. The results showed collagen hydrogels have good biocompatibility, and cell proliferation increased after collagen type II treatment. Expressions of SOX9, aggrecan, and collagen type II were increased in a collagen type II dependent manner. Sulfate proteoglycan synthesis increased in proportion to collagen type II concentration. Only hADMSCs highly expressed NP cell marker KRT19 in collagen type II culture. Additionally, phosphorylated Smad3, which is associated with phosphorylated ERK, was increased after collagen type II‐stimulation. The concentration and type of collagen affect hADMSC differentiation into NP cells. Collagen type II significantly ameliorates hADMSC differentiation into NP cells and promotes extracellular matrix synthesis. Therefore, anabolism of collagen type I and catabolism of type II may attenuate the differentiation and biosynthesis of transplanted stem cells. © 2016 BioFactors, 42(2):212–223, 2016
Choice of fusion proteins, expression host, and analytics solves difficult‐to‐produce protein challenges in discovery research
Authors: Lyons‐Abbott Sally , Abramov Ariel , Chan Chung‐leung , Deer Jen Running , Fu Guangsen , Hassouneh Wafa , Koch Tyree , Misquith Ayesha , O'Neill Jason , Simon Sandy Alexander , Wolf Anitra , Yeh Ronald , Vernet Erik 
Published: October 2023

AbstractHigh quality biological reagents are a prerequisite for pharmacological research. Herein a protein production screening approach, including quality assessment methods, for protein‐based discovery research is presented. Trends from 2895 expression constructs representing 253 proteins screened in mammalian and bacterial hosts—91% of which are successfully expressed and purified—are discussed. Mammalian expression combined with the use of solubility‐promoting fusion proteins is deemed suitable for most targets. Furthermore, cases utilizing stable cell line generation and choice of fusion protein for higher yield and quality of difficult‐to‐produce proteins (Leucine‐rich repeat‐containing G‐protein coupled receptor 4 (LGR4) and Neurturin) are presented and discussed. In the case of Neurturin, choice of fusion protein impacted the target binding 80‐fold. These results highlight the need for exploration of construct designs and careful Quality Control (QC) of difficult‐to‐produce protein reagents.
Adaptive laboratory evolution and transcriptomics‐guided engineering of Escherichia coli for increased isobutanol tolerance
Authors: Jang Young Seo , Yang Jungwoo , Kim Jae Kyun , Kim Tae In , Park Yong‐Cheol , Kim In Jung , Kim Kyoung Heon 
Published: October 2023

AbstractAs a renewable energy from biomass, isobutanol is considered as a promising alternative to fossil fuels. To biotechnologically produce isobutanol, strain development using industrial microbial hosts, such as Escherichia coli, has been conducted by introducing a heterologous isobutanol synthetic pathway. However, the toxicity of produced isobutanol inhibits cell growth, thereby restricting improvements in isobutanol titer, yield, and productivity. Therefore, the development of robust microbial strains tolerant to isobutanol is required. In this study, isobutanol‐tolerant mutants were isolated from two E. coli parental strains, E. coli BL21(DE3) and MG1655(DE3), through adaptive laboratory evolution (ALE) under high isobutanol concentrations. Subsequently, 16 putative genes responsible for isobutanol tolerance were identified by transcriptomic analysis. When overexpressed in E. coli, four genes (fadB, dppC, acs, and csiD) conferred isobutanol tolerance. A fermentation study with a reverse engineered isobutanol‐producing E. coli JK209 strain showed that fadB or dppC overexpression improved isobutanol titers by 1.5 times, compared to the control strain. Through coupling adaptive evolution with transcriptomic analysis, new genetic targets utilizable were identified as the basis for the development of an isobutanol‐tolerant strain. Thus, these new findings will be helpful not only for a fundamental understanding of microbial isobutanol tolerance but also for facilitating industrially feasible isobutanol production.
Four Claims on Research Assessment and Metric Use in the Humanities
Authors: Hammarfelt Björn 
Published: October 2023

EDITOR'S SUMMARYBibliometric evaluation for research in the field of sciences can be a good way to assess the quality and factual basis of claims and can lead to more funding for authors and for research work. However, due to the more diverse fields covered, this type of evaluation is less effective in the world of humanities. Many professionals and researchers in humanities fields believe that bibliometric evaluation is meant only for STEM research and can't properly assess any findings made in humanities. Four common claims made about bibliometrics in humanities are that bibliometrics do not adequately cover the non‐uniform nature of humanities; greater bibliometric coverage will not solve all the research problems in humanities subjects; metrics use already has an impact on humanities research practices and finally; other evaluation methods, like altmetrics, are conventional.
Chemical Profiling of Nitraria roborowskii Kom. by UPLC‐Q‐Orbitrap‐MS and Their Hypolipidemic Effects in Vivo
Authors: Jia Qiangqiang Q. , Yang Zufan F. , Wang Qian , Zhao Qing , Jia Yujiao J. , Guo Banghao H. , Li Xiangyang Y. , Wang Wen 
Published: October 2023

AbstractThe Nitraria roborowskii Kom. (NRK) berries, as fruits of the genus Nitraria of the Zygophyllceae family, have been widely used as folk medicine. Modern pharmacological research has demonstrated that Nitraria berries had hypolipidemic, hypoglycemic, and immunomodulatory effects. However, more research needs to be reported on the chemical composition and biological activity of NRK. Hence, the phenolic compounds in the NRK berries were comprehensively analyzed and characterized by Ultra Performance Liquid Chromatography‐Quadruple‐Orbitrap MS system (UPLC‐Q‐Orbitrap MS) in this study. In total, 52 phenolics were identified, and all were reported for the first time. In addition, the hypolipidemic efficacy of NRK berries extract was studied in the hyperlipidemic mouse model. After treatment, the high dose group of NRK substantially reversed total cholesterol, low‐density lipoprotein cholesterol, and high‐density lipoprotein cholesterol levels. Through lipidomics technology, 27 potential biomarkers were characterized. And there was a significant callback at 25 of them after NRK treatment by using statistical analysis methods. Pathway analysis results demonstrated that NRK might exert therapeutic effects by regulating glycerophospholipid and glycerolipid metabolism pathways. This study could provide firsthand information on NRK berries for their phenolic compounds and potential application in preventing and treating hyperlipidemia.
Efficient Oxidation of 5‐Hydroxymethylfurfural Using a Flavoprotein Oxidase from the Honeybee Apis mellifera
Authors: Tjallinks Gwen , Boverio Alessandro , Jager Amarins W. , Kaya Saniye G. , Mattevi Andrea , Fraaije Marco W. 
Published: October 2023

AbstractThe chemical 5‐hydroxymethylfurfural (HMF) can be derived from lignocellulose and is an interesting bio‐based platform chemical as it has the potential to be transformed into numerous valuable building blocks such as the polymer‐precursor 2,5‐diformylfuran (DFF). To date, only a few oxidases acting on HMF are known and by sampling atypical species, we discovered a novel flavin‐dependent oxidoreductase from the honeybee Apis mellifera (beeHMFO). The enzyme can perform the chemoselective oxidation of HMF to DFF but can also readily accept other aromatic alcohols as substrates. The function of the enzyme may well be the antimicrobial generation of hydrogen peroxide using HMF, which is very abundant in honey. The discovery of this insect‐derived flavoprotein oxidase holds promising potential in the synthesis of renewable products and demonstrates that insects can be an interesting source of novel biocatalysts.
Protracted complete response after limited checkpoint inhibitor dosing: A case report
Authors: Sember Quinne , Sigal Darren 
Published: October 2023

AbstractImmunotherapy is an important modality in the treatment of many types of malignancy. The optimal duration of treatment with immunotherapy in patients with metastatic malignancies who experience complete responses is not fully understood. Our case demonstrates a protracted complete response in a patient with metastatic gastric adenocarcinoma after just three doses of pembrolizumab. This illustrates a need for further research into which patients might have responses such as these and could therefore be spared additional doses of an expensive treatment with many potential side effects.
Iron‐catalyzed Hydrosilylation of Secondary Carboxamides: Chemoselective Access to Aldimines
Authors: Wu Jiajun , Narayanasamy Subash Nethaji , Darcel Christophe 
Published: October 2023

AbstractThis contribution described the chemoselective reduction of secondary carboxamides to aldimines. To perform such challenging transformation, we reported a catalyzed hydrosilylation using Fe(CO)4(IMes) [IMes=1,3‐bis (2,4,6‐trimethylphenyl) imidazol‐2‐ylidene] as the catalyst, diphenylsilane as the reductant under UV irradiation (350 nm) at room temperature for 16 h. Aldimines were then obtained, after a basic quench, in 32–83 % isolated yields. This transformation was unprecedented at iron.
Durable K‐ion batteries with 100% capacity retention up to 40,000 cycles
Authors: Lu Xianlu , Liang Zhao , Fang Zhi , Zhang Dongdong , Zheng Yapeng , Liu Qiao , Fu Dingfa , Teng Jie , Yang Weiyou 
Published: October 2023

AbstractCurrently, the major challenge in terms of research on K‐ion batteries is to ensure that they possess satisfactory cycle stability and specific capacity, especially in terms of the intrinsically sluggish kinetics induced by the large radius of K+ ions. Here, we explore high‐performance K‐ion half/full batteries with high rate capability, high specific capacity, and extremely durable cycle stability based on carbon nanosheets with tailored N dopants, which can alleviate the change of volume, increase electronic conductivity, and enhance the K+ ion adsorption. The as‐assembled K‐ion half‐batteries show an excellent rate capability of 468 mA h g−1 at 100 mA g−1, which is superior to those of most carbon materials reported to date. Moreover, the as‐assembled half‐cells have an outstanding life span, running 40,000 cycles over 8 months with a specific capacity retention of 100% at a high current density of 2000 mA g−1, and the target full cells deliver a high reversible specific capacity of 146 mA h g−1 after 2000 cycles over 2 months, with a specific capacity retention of 113% at a high current density of 500 mA g−1, both of which are state of the art in the field of K‐ion batteries. This study might provide some insights into and potential avenues for exploration of advanced K‐ion batteries with durable stability for practical applications.
Capture‐SELEX of DNA Aptamers for Sulforhodamine B and Fluorescein
Authors: Gu Lide , Zheng Jiajie , Zhang Yao , Wang Deli , Liu Juewen 
Published: October 2023

AbstractWhile many dye binding aptamers have been reported, most of them were for light‐up aptamers that can significantly enhance the quantum yield of fluorophores. Sulforhodamine B (SRhB) was used as a target previously to select both DNA and RNA aptamers, and the DNA aptamer was a G‐quadruplex that can bind to a number of rhodamine analogs. In addition, the previous selections were performed by immobilizing the target molecules. In this work, the library immobilization method was used to respectively select aptamers for SRhB and fluorescein. The SRhB aptamer has a non‐G‐quadruplex structure with a Kd of 1.0 μM measured from isothermal titration calorimetry. Upon titration of the aptamer, the fluorescence of SRhB increased 2.5‐fold, and this aptamer does not require Mg2+ for binding. Rhodamine B has even tighter binding suggesting binding through the xanthene moiety of the dyes. No binding was detected for fluorescein. For the fluorescein selection, a dominant aptamer sequence with a Kd of 147 μM was obtained. This study provides two new aptamers for two important fluorophores that can be used to study aptamer‐based separation, dye detection and catalysis. Comparison of these aptamers also provides insights into the effect of functional groups on aptamer binding.
Expression of B7‐H3 and TIM‐3 in gastric‐type endocervical adenocarcinoma: prevalence, association with PD‐L1 expression, and prognostic significance
Authors: Sun Yao , Zhou Xin , Lucas Elena , Chen Lili , Zhang Huijuan , Chen Hao , Zhou Feng 
Published: October 2023

AbstractGastric‐type endocervical adenocarcinoma (GEA) is the second most common subtype of endocervical adenocarcinoma and has a poor prognosis. Anti‐programmed death‐1 and anti‐programmed death‐ligand 1 (PD‐L1) inhibitors have emerged as a major treatment option for GEA; however, data on the expression of other immune checkpoints in GEA are limited. We analyzed the expression of T‐cell immunoglobulin and mucin‐domain containing‐3 (TIM‐3) and B7 homolog 3 protein (B7‐H3) in 58 GEA and investigated their prognostic significance as well as association with PD‐L1 expression and other known prognostic factors. Applying the tumor proportion score (TPS) with a cutoff of 1%, B7‐H3 and TIM‐3 were present in 48.3% and 17.2% of cases, respectively. Applying the combined positive score (CPS) with a cutoff of 1, TIM‐3 expression was present in 70.7% of cases. Moreover, the expression of three checkpoints (B7‐H3, TIM‐3, and PD‐L1) was incompletely overlapping. Patients with B7‐H3 positive tumors (by TPS) or TIM‐3 positive tumors (by TPS) had significantly worse recurrence‐free survival (RFS) and overall survival (OS) (log‐rank). Using CPS, patients with TIM‐3 positive tumors showed significantly worse RFS (log‐rank). Similarly, B7‐H3 positivity (by TPS) and TIM‐3 positivity (by TPS) were associated with worse RFS and OS in univariate analysis. TIM‐3 positivity (by CPS) was associated with worse RFS in univariate analysis and the final Cox multivariate analysis. In conclusion, our results show that (1) B7‐H3 and TIM‐3 are frequently expressed in GEA and their expression overlaps incompletely with PD‐L1; and (2) both B7‐H3 and TIM‐3 are independent negative prognostic markers in GEA.