bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant chimeric Horsepox Virus (TNX-801) is attenuated relative to Vaccinia Virus Strains in Human Primary Cell Lines and in Immunocompromised Mice
Authors: Trefry Stephanie V , Raney Christy N , Cregger Amy L , Gonzales Chase A , Layton Brittney L , Enamorado Robert N , Martinez Nelson A , Gohegan Deborah S , Moulaei Tinoush , Ziółkowska Natasza E , Goebel Scott J , Lederman Seth , Bavari Sina , Nasar Farooq 
Published: October 2023

AbstractRecombinant chimeric Horsepox virus (TNX-801) is a preclinical vaccine in development against Monkeypox and smallpox. In this brief report, we investigated the potential phenotypic differences inin vitroandin vivomodels between TNX-801 and older VACV-based vaccine strains (VACV-IHD, VACV-Lis, VACV-NYC) used in the eradication of smallpox virus. TNX-801 displayed a small plaque phenotype (∼1-2 mm) in both BSC-40 and Vero-E6 cells and yielded >10- to 100-fold lower infectious titers than the VACV strains in multiple-step replication kinetics. Growth kinetics in primary human cell lines from two main routes of poxvirus transmission, respiratory and dermal tracts, yielded ∼10- to 119-fold lower infectious titers of TNX-801. Intranasal infection of immunocompromised mice (C56BL/6Ifnar−/−/Ifngr−/−) with VACV strains at 6.0 and 5.0 log10PFU produced uniform lethal disease. In contrast, TNX-801 at 8.0 log10PFU was unable to produce any clinical disease in mice. These data demonstrate that TNX-801 is >10- to 1,000-fold more attenuated than older VACV-based smallpox vaccines in human primary cell lines and immunocompromised mice.
Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system
Authors: Otto Simon B. , Servajean Richard , Lemopoulos Alexandre , Bitbol Anne-Florence , Blokesch Melanie 
Published: October 2023

AbstractThe bacterial type VI secretion system (T6SS) is a widespread, kin-discriminatory weapon capable of shaping microbial communities. Due to the system’s dependency on contact, cellular interactions can lead to either competition or kin protection. Cell-to-cell contact is often accomplished via surface-exposed type IV pili (T4P). InVibrio cholerae,these T4P facilitate specific interactions when the bacteria colonize natural chitinous surfaces. However, it has remained unclear whether and, if so, how these interactions affect the bacterium’s T6SS-mediated killing. In this study, we demonstrate that pilus-mediated interactions can be harnessed to reduce the population ofV. choleraeunder liquid growth conditions in a T6SS-dependent manner. We also show that the naturally occurring diversity of pili determines the likelihood of cell-to-cell contact and, consequently, the extent of T6SS-mediated competition. To determine the factors that enable or hinder the T6SS’s targeted reduction of competitors carrying pili, we developed a physics-grounded computational model for autoaggregation. Collectively, our research demonstrates that T4P involved in cell-to-cell contact can impose a selective burden whenV. choleraeencounters non-kin cells that possess an active T6SS. Additionally, our study underscores the significance of T4P diversity in protecting closely related individuals from T6SS attacks through autoaggregation and spatial segregation.
Assessing the causal effect of air pollution on risk of SARS-CoV-2 infection
Authors: Navaratnam Annalan M D , Beale Sarah , Boukari Yamina , Nguyen Vincent , Fong Wing Lam Erica , Braithwaite Isobel , Byrne Thomas E , Fragaszy Ellen , Geismar Cyril , Kovar Jana , Patel Parth , Shrotri Madhumita , Yavlinsky Alexei , Hayward Andrew C , Khreis Haneen , Aldridge Robert W 
Published: October 2023

AbstractIntroductionEmerging evidence suggests association of air pollution exposure with risk of SARS-CoV-2 infection, but many of these findings are limited by study design, lack of individual-level covariate data or are specific to certain subpopulations. We aim to evaluate causal effects of air pollution on risk of infection, whilst overcoming these limitations.MethodsConcentrations for black carbon(BC), particulate matter 10(PM10), particulate matter 2.5(PM2.5), nitrogen dioxide(NO2) and oxides of nitrogen(NOx) from the Department of Environment, Food and Rural Affairs (DEFRA) and Effect of Low-level Air Pollution: A Study in Europe (ELAPSE) were linked to postcodes of 53,683 Virus Watch study participants. The primary outcome was first SARS-CoV-2 infection, between 1st September 2020 and 30th April 2021. Regression analysis used modified Poisson with robust estimates, clustered by household, adjusting for individual (e.g., age, sex, ethnicity) and environmental covariates(e.g., population density, region) to estimate total and direct effects.ResultsSingle pollutant analysis showed the direct effect of higher risk of SARS-CoV-2 infection with increased exposure to PM2.5(RR1.11,95%CI 1.08;1.15), PM10(RR1.06,95%CI 1.04;1.09), NO2(RR1.04,95%CI 1.04;1.05) and NOx(RR1.02,95%CI 1.02;1.02) per 1µg/m3increment with DEFRA 2015-19 data. Sensitivity analyses altering covariates, exposure window and modelled air pollution data source produced similar estimates. Higher risk of SARS-CoV-2 per 10-5m-1increment of BC (RR1.86, 95%CI 1.62;2.14) was observed using ELAPSE data.ConclusionLong term exposure to higher concentrations of air pollutions increases the risk of SARS-CoV-2 infection, highlighting that adverse health effects of air pollution is not only limited to non-communicable diseases.
Clinical performance of automated machine learning: a systematic review
Authors: Thirunavukarasu Arun James , Elangovan Kabilan , Gutierrez Laura , Hassan Refaat , Li Yong , Tan Ting Fang , Cheng Haoran , Teo Zhen Ling , Lim Gilbert , Ting Daniel Shu Wei 
Published: October 2023

AbstractIntroductionAutomated machine learning (autoML) removes technical and technological barriers to building artificial intelligence models. We aimed to summarise the clinical applications of autoML, assess the capabilities of utilised platforms, evaluate the quality of the evidence trialling autoML, and gauge the performance of autoML platforms relative to conventionally developed models, as well as each other.MethodsThis review adhered to a PROSPERO-registered protocol (CRD42022344427). The Cochrane Library, Embase, MEDLINE, and Scopus were searched from inception to 11 July 2022. Two researchers screened abstracts and full texts, extracted data and conducted quality assessment. Disagreement was resolved through discussion and as-required arbitration by a third researcher.ResultsIn 82 studies, 26 distinct autoML platforms featured. Brain and lung disease were the most common fields of study of 22 specialties. AutoML exhibited variable performance: AUCROC 0.35-1.00, F1-score 0.16-0.99, AUCPR 0.51-1.00. AutoML exhibited the highest AUCROC in 75.6% trials; the highest F1-score in 42.3% trials; and the highest AUCPRC in 83.3% trials. In autoML platform comparisons, AutoPrognosis and Amazon Rekognition performed strongest with unstructured and structured data respectively. Quality of reporting was poor, with a median DECIDE-AI score of 14 of 27.ConclusionsA myriad of autoML platforms have been applied in a variety of clinical contexts. The performance of autoML compares well to bespoke computational and clinical benchmarks. Further work is required to improve the quality of validation studies. AutoML may facilitate a transition to data-centric development, and integration with large language models may enable AI to build itself to fulfil user-defined goals.
Mendelian randomization study of birth weight and risk of psychiatric disorders later in life
Authors: Wu Xiaoping , Geller Frank , Helenius Dorte , Grove Jakob , Albiñana Clara , Petersen Liselotte Vogdrup , Bulik Cynthia M. , Børglum Anders D. , Werge Thomas , Vilhjálmsson Bjarni J. , Feenstra Bjarke 
Published: October 2023

AbstractLow birth weight has been associated with a higher risk of psychiatric disorders later in life. The underlying causal mechanisms of this relationship are however not clear. In this study, we investigate whether variation in fetal growth has a direct causal effect on mental health. Using birth weight as a proxy measure for fetal growth, we first assessed associations between observed birth weight and later diagnosis of psychiatric disorders in the Danish iPSYCH and ANGI-DK cohorts. Next, we constructed a polygenic score for birth weight based on >1 million variants and tested for association with psychiatric disorders. Finally, using 86 single-nucleotide polymorphisms with robust fetal-only genetic associations with birth weight, we assessed the causal relationship of genetically mediated fetal growth and psychiatric disorders using Mendelian randomization analyses. We found that higher observed birth weight was associated with lower risk of several psychiatric disorders. Polygenic score analyses supported this pattern for attention deficit/hyperactivity disorder, where an increase of one standard deviation in the score for birth weight corresponded to an odds ratio of 0.85 (95% confidence interval 0.79-0.92, P=6.27×10-5). However, one- and two-sample Mendelian randomization analyses did not indicate a direct causal relationship between the birth weight of children and their risk of psychiatric disorders. In conclusion, our study does not support a direct causal effect of fetal growth (as proxied by birth weight) on the risk of psychiatric disorders later in life, suggesting that the observed association between birth weight and mental health is likely to be caused by other factors.
Interactions of netrin-1 through its glycosylation sites immobilize Deleted in Colorectal Cancer (DCC) by favoring its constitutive clustering
Authors: Uriot Karen , Blanc Olivier , Audugé Nicolas , Faklaris Orestis , Blanc Olivier , Chaverot Nathalie , Bloch-Gallego Evelyne , Borghi Nicolas , Coppey-Moisan Maïté , Girard Philippe P. 
Published: October 2023

AbstractNetrin-1 is a protein that attracts neurons expressing the membrane receptor Deleted in Colorectal Cancer DCC. In colon carcinoma, the interaction between netrin-1 and DCC prevents apoptosis. Crystallographic data suggest that these processes involve the clustering of DCC, the observation of which in cells remains elusive, as do the molecular determinants of DCC-netrin-1 interactions and their impact on DCC organization and mobility. To address these questions, we used fluorescence photobleaching, single-particle tracking and super-resolution techniques to characterize DCC organization and mobility on the cell surface. Our results show that netrin-1 impedes DCC mobility in the plasma membrane by promoting the growth of constitutive DCC nanoclusters at the expense of free DCC. Furthermore, we show that these effects are mediated primarily by the three glycosylation sites in the LamVI domain of netrin-1 and, to a lesser extent, by the C-terminal domain and its RGD binding site.
Rationale design of unrestricted pRN1 derivatives and their application in construction of a dual plasmid vector system forSaccharolobus islandicus
Authors: Zhao Pengpeng , Bi Xiaonan , Wang Xiaoning , Feng Xu , Shen Yulong , Yuan Guanhua , She Qunxin 
Published: October 2023

AbstractSaccharolobus islandicusREY15A represents one of the very few archaeal models with versatile genetic tools, including efficient genome editing, gene silencing and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are solely based on the pRN2 cryptic plasmid. Although this plasmid co-exists with pRN1 in their original host, early attempts to test pRN1-based vectors consistently failed to yield any stable host-vector system forSa. islandicus. Herein we identified a putative target sequence inorf904encoding a putative replicase on pRN1 (TargetN1). Mutated targets were then designed (N1a, N1b, N1c) and tested for their capability of escaping from the host CRISPR immunity by using plasmid interference assay. This revealed that the original target triggers the CRISPR immunity in this archaeon whereas all three mutated targets do not, indicating that all designed target mutations evade the host immunity. These mutated targets were then incorporated intoorf904individually, yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed (pN1aSD, pN1bSD and pN1cSD).Sa. islandicustransformation revealed that pN1aSD and pN1bSD were functional shuttle vectors, but pN1cSD lost the capability of replication. In addition, pRN1-based and pRN2-based vectors were stably maintained in the archaeal cells either alone or in combination, and this yielded a dual plasmid system for genetic study with this important archaeal model.Impact statementWhen pRN1 was employed for vector construction inSaccharolobus islandicusREY15A, pRN1-derived vectors are not stable in this archaeon. Here we show that pRN1 orf904 encoding a putative replicase on pRN1 carries a DNA segment to be targeted by the host I-A CRISPR system. By designing mutated target sequences that evade the CRISPR immunity, efficient plasmid vectors were obtained with mutated pRN1 backbones. This strategy could be applied in developing host-vector systems for other microorganisms with plasmids or viruses carrying CRISPR target sequences. Moreover, the resulting dual vector system would facilitate genetic studies with this crenarchaeal model.
Transdifferentiation of fibroblasts into muscle cells to constitute cultured meat with tunable intramuscular fat deposition
Authors: Ma Tongtong , Ren Ruimin , Lv Jianqi , Yang Ruipeng , Xinyi Zheng , Hu Yang , Zhu Guiyu , Wang Heng 
Published: October 2023

AbstractCurrent studies on cultured meat mainly focused on the muscle tissue reconstruction in vitro, but lack the formation of intramuscular fat which is a crucial factor in determining taste, texture and nutritional contents. Therefore, incorporating fat into cultured meat is of superior value. In this study, we employed the myogenic/lipogenic transdifferentiation of chicken fibroblasts in 3D to produce muscle mass and deposit fat into the same cells without the co-culture or mixture of different cells or fat substances. The immortalized chicken embryonic fibroblasts were implanted into the hydrogel scaffold and the cell proliferation and myogenic transdifferentiation were conducted in 3D to produce the whole-cut meat mimics. Compare to 2D, cells grown in 3D matrix showed elevated myogenesis and collagen production. We further induced fat deposition in the transdifferentiated muscle cells and the triglyceride content could be manipulated to match and exceed the levels of chicken meat. The gene expression analysis indicated that both lineage-specific and multi-functional signalings could contribute to the generation of muscle/fat matrix. Overall, we were able to precisely modulate muscle, fat, and extracellular matrix contents according to balanced or specialized meat preferences. These findings provide new avenues for customized cultured meat production with desired intramuscular fat contents that can be tailored to meet the diverse demands of consumers.Graphical Abstract
Enterprise architecture for the transformation of public services based on citizen’s feedback
Authors: Singh Priyanka , Lynch Fiona , Helfert Markus 
Published: October 2023

Purpose
Current literature argues that citizen engagement platforms must be used to gather citizens’ feedback to provide improved quality of services to citizens. However, limited studies consider the challenges faced by practitioners at the local level during the incorporation of those feedback for continuous service improvement. As a result, these services fail to fulfil the need of citizens. The purpose of this study is to structure the relationship between citizens’ feedback and continuous service improvement to meet the need of citizens.

Design/methodology/approach
Design science research methodology has been adapted under which a case study approach has been followed to investigate one of the citizens’ engagement platforms in Ireland.

Findings
The results from this study highlighted that practitioners faced challenges (e.g. capacity, risk and constraints) in terms of fulfilling the needs of citizens and there is a lack of structured approach to continuously provide improved services to them.

Research limitations/implications
This study provides a structured approach in the form of a process model to showcase how citizens’ feedback can be incorporated for continuously providing improved services to the citizens.

Social implications
This research provides a prescriptive view to assist municipalities during the incorporation of citizens’ feedback for continuous service improvement while addressing the challenges they face during this process.

Originality/value
This paper proposes a process model based on the guidelines of the open group architecture framework enterprise architecture and the collaboration with practitioners that would assist local authorities in continuously providing improved services to the citizens.
A scientometrics analysis of equity, diversity, inclusion, and accessibility (EDIA) literature in library and information science profession
Authors: Ashiq Murtaza , Ur Rehman Shafiq , Warraich Nosheen Fatima 
Published: October 2023

Purpose
Equity, diversity, inclusion and accessibility (EDIA) are the core values of librarianship. This study aims to examine the EDIA literature in the library and information science (LIS) profession, focusing on publishing trends, authorship patterns, thematic evolution, co-occurrence network, international collaboration network and three-factor analysis.

Design/methodology/approach
A scientometrics analysis was performed, and data were retrieved from the Web of Science database following a four-phase data extraction and filtration process. Data analysis was performed using specialized scientometrics tools.

Findings
Most of the published work comes from the developed world. The thematic evolution identified that the initial period (1971–2010) was dedicated to the diversity of library resources, services and users. The second phase (2011–2015) concentrated on accessibility and information literacy. The recent period (2016–2021) is linked with social inclusion, diversity and accessibility. Furthermore, the co-cluster citation and content analysis identified five major interconnected streams in the EDIA literature in LIS: disability and accessibility; diversity, inclusion and recruitment; social justice and libraries; libraries and immigrants; and libraries and the lesbian, gay, bisexual, transgendered, queer community.

Research limitations/implications
Theoretical, practical and implications for policy makers and curriculum developers were added to create awareness and better observe EDIA in LIS field.

Originality/value
The identification of research streams is one of the most important findings of this study, which shows that some areas of EDIA are maturing and others are emerging in the profession.
“Buy local” campaigns in times of crisis: insights from reactance theory
Authors: Matarazzo Michela , Diamantopoulos Adamantios , Raff Andreas 
Published: October 2023

PurposeReactance theory is applied to investigate consumer responses to “buy local” campaigns initiated by government to counteract the effects of an economic crisis, using the COVID-19 pandemic as an illustrative context.Design/methodology/approachA conceptual model is developed, aimed at revealing the extent to which “buy local” campaigns – explicitly justified by the need to fight an economic crisis – are likely to lead to (a) compliance (i.e. support for local products/retailers) or (b) freedom restoration (i.e. support for foreign products/retailers). The model is subsequently tested on samples of German (N = 265) and Italian (N = 268) consumers.Findings“Buy local” campaigns are likely to generate reactance amongst consumers and such reactance can lead to both non-compliance and, albeit less so, freedom restoration outcomes. At the same time, consumer ethnocentrism acts as a countervailing influence by attenuating the effects of generated reactance and its undesirable outcomes.Research limitations/implicationsPsychological reactance theory offers a novel perspective for conceptually approaching the likely responses of consumers towards “buy local” campaigns and the empirical findings support the use of the theory in this context.Practical implicationsPolicymakers seeking to encourage consumers to support the local economy during times of an economic crisis need to be aware that “buy local” campaigns may, against their intended communication goals, result in non-compliance as well as consumer responses in the opposite direction. Thus, the reactance-generating potential of such campaigns needs to be explicitly considered at the planning/implementation stage.Originality/valueThe findings confirm the relevance of reactance theory as a conceptual lens for studying the effects of “buy local” campaigns and have important implications for domestic/foreign firms as well as for policy makers seeking to encourage consumers to support the local economy during times of an economic crisis.
Relevance and its epistemic underpinnings in B2B marketing research: four axioms and nine relevance types
Authors: Ojansivu Ilkka Tapani 
Published: October 2023

Purpose
This study aims to explore what characteristics contribute to the definition of relevance in business-to-business (B2B) marketing research and how/why different strands of B2B marketing maintain or lose their relevance.

Design/methodology/approach
This study is conceptual. It adopts a performative-phenomenal standpoint for B2B marketing research and approaches relevance through the concept of episteme, which is considered pivotal for understanding this phenomenon.

Findings
This study proposes four axioms that define the characteristics of relevance in B2B marketing research and discusses their implications for scholars and practitioners. Consequently, an action plan for revitalizing B2B marketing research is developed, comprising learning and temporal dimensions, resulting in nine different relevance types.

Research limitations/implications
The central argument put forward in this study is that different research strands of B2B marketing have deeply rooted epistemic underpinnings that influence their interpretation of relevance. Consequently, fostering dialogue between practitioners and scholars is considered necessary to sustain relevance in B2B marketing research. B2B scholars are urged to think beyond their subspecialized silos and acknowledge how the business environment and the various strands of B2B marketing congruently shape B2B marketing relevance, while also embracing research methods that bring them closer to business practice.

Practical implications
Marketing practitioners and academics continue to drift apart. This study puts forward three recommendations to bring marketing academics and practitioners closer together.

Originality/value
The study contributes to the B2B marketing literature by grappling with the theory-praxis gap and critically exploring what constitutes relevance in B2B marketing research.