bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prey or protection? Access to food alters individual responses to competition in black widow spiders
Authors: Ratz Tom , Montiglio Pierre-Olivier 
Published: February 2025

Abstract
Animals influence the phenotype and reproductive success of their conspecifics through competitive interactions. Such effects of competition can alter the intensity of selection and ultimately change the rate of evolution. However, the magnitude of the effects of competition, and their evolutionary impact, should vary depending on environmental conditions and individual responses among competitors. We tested whether a key environmental variable, resource availability, affects the response to competition in black widow spiders by manipulating access to prey and the level of competition. We examined if focal spiders modify their web structure and aggressiveness towards prey stimuli when a competitor is present, and whether these responses depend on prior prey access. We also tested if any effects of competition vary with individual differences among competitors. Access to resources changed how individuals respond to competition. Spiders with limited access to prey were less likely to attack prey stimuli in the presence of a conspecific competitor than spiders with greater access to prey, suggesting that limiting resources hinders competitive responses. In contrast, all spiders built better-protected webs in the presence of competitors, regardless of prior access to prey. Crucially, these responses differed among focal spiders and depended on individual competitors. Our findings highlight the importance of environmental conditions and individual differences in mediating the impact of social interactions on phenotypes and eventually on their evolution.
Development of an optimized cell-based selection system for phage display libraries
Authors: Czarnecka Malgorzata , Findik Nicole , Schlör Anja , Hanack Katja 
Published: February 2025

Abstract
The discovery of antibodies through phage display is significantly influenced by antigen presentation during panning, particularly for membrane-anchored proteins, which pose challenges due to their complex structures. Traditional approaches, such as whole cells expressing the target protein, often result in low antigen density and high background signals. In this study, we describe an alternative method using stably transfected cell lines that express the target antigen on their surface, regulated by an intracellular enhanced green fluorescent protein (EGFP) signal. This system enables high-throughput flow cytometry-based screening of phage display libraries to isolate human antibodies that recognize the native conformation of membrane proteins. Using human epithelial cell adhesion molecule (EpCAM) and human neuroplastin 65 (NP65) as model antigens, we established an optimized screening workflow with polyclonal phage pools. Selected EpCAM-specific single-chain variable fragments (scFvs) from a naïve library were recombinantly expressed with an IgG4 scaffold and characterized for specific binding. This approach provides an effective platform for the identification of antibodies against membrane proteins in their native state.
RUNCOV: a one-pot triplex real-time RT-LAMP as a point-of-care diagnostic tool for detecting SARS-CoV-2
Authors: Robène Isabelle , Jouen Emmanuel , Maillot-Lebon Véronique , Fenelon Babbitha T , Hascoat Jérémy , Pecrix Yann , Richard Damien , Jaffar-Bandjee Marie-Christine , Mavingui Patrick , Chiroleu Frédéric , Becker Nathalie , Poubeau Patrice , Ramiandrisoa Mahery , Sin Michel , Costet Laurent , Laurent Annie , Laurent Philippe , Chabirand Aude , Moreau Aurélie , Reynaud Bernard , Jeuffrault Eric , Cetre Sossah Catherine 
Published: February 2025

Abstract
Given the risk of zoonotic disease emergence, including new SARS-CoV-2 variants of COVID-19, rapid diagnostic tools are urgently needed to improve the control of the spread of infectious diseases. A one-pot triplex real-time RT-LAMP (reverse-transcription-loop-mediated isothermal amplification) assay, based on two regions of the genome SARS-CoV-2 - specifically the Orf1ab and N genes, along with one internal control, the human RNase P gene, was developed. The multiplexing relies on the distinct melting peaks produced during an annealing step. This tool, named RUNCOV, was compared to the gold standard reverse-transcription real-time quantitative PCR (RT-qPCR) assay. A simple sample preparation step was designed alongside the assay, making it ready for use on site, as a point-of-care diagnostic tool. RUNCOV is rapid (typically less than 40 minutes), highly sensitive and specific. When tested on clinical samples with known SARS-CoV-2 status, its limit of detection (LOD) ranges between 5-20 copies per reaction and its diagnostic sensitivity (97.44%) and specificity (100%) values are high compared to the RT-qPCR gold standard. These results were supported with an extensive in silico analysis of over 14 million genomes, demonstrating this tool was capable of detecting all known SARS-CoV-2 variants, including the most recent ones KP.3.1.1 and BA2.86.1. This molecular assay is portable, as demonstrated when it was used successfully in La Réunion in different contexts outside the laboratory.
Inclusive science communication as a tool to address One Health challenges
Authors: Kelp Nicole 
Published: February 2025

Abstract
Solving the multisectoral, complex challenges involved in One Health requires inclusive collaboration and communication. Unfortunately, many natural scientists have not been trained in the social science research that informs effective science communication. In this Overview, I review the principles of inclusive science communication—intentionality, reflexivity, and reciprocity—and discuss how these principles can promote more effective communication with communities about One Health topics. In addition, I implicate the need for equitable science policy to support inclusive science communication. Finally, I provide a framework of self-reflection questions for scientists to grow as reflective and effective science communicators.
Hemispheric asymmetry of the white matter microstructure in schizophrenia patients with persistent auditory verbal hallucinations
Authors: Li Jinguang , He Jingqi , Ren Honghong , Li Chunwang , Li Zongchang , Chen Xiaogang , He Ying , Tang Jinsong 
Published: February 2025

Abstract

The link between hemispheric asymmetry and auditory verbal hallucinations in schizophrenia is underexplored with neuroimaging evidence. This study examined white matter asymmetries in schizophrenia patients. Diffusion tensor imaging data from 52 patients with persistent auditory verbal hallucinations, 33 who never experienced auditory verbal hallucinations, and 40 healthy controls were analyzed. Asymmetry indices for fractional anisotropy, axial diffusivity, radial diffusivity, and mean diffusivity were calculated for the whole-brain white matter skeleton and 22 pairs of regions of interest. The persistent auditory verbal hallucination group showed reduced fractional anisotropy asymmetry index in the whole-brain white matter skeleton compared to healthy control and never experienced auditory verbal hallucination groups, indicating altered asymmetry. Region of interest analysis revealed decreased fractional anisotropy asymmetry index in nine pairs and increased mean diffusivity AI in two pairs in the persistent auditory verbal hallucination group. Greater rightward asymmetry in the superior longitudinal fasciculus correlated with more severe auditory verbal hallucinations in persistent auditory verbal hallucination patients. No significant asymmetry differences were found between never experienced auditory verbal hallucinations and healthy control groups. Ridge regression analysis demonstrated that including the fractional anisotropy asymmetry index of the superior longitudinal fasciculus increased the explained variance in auditory verbal hallucination severity. These findings highlight distinct white matter asymmetry patterns in persistent auditory verbal hallucination patients, suggesting that hemispheric asymmetry plays a key role in the pathology of auditory verbal hallucinations in schizophrenia.

Highlights
J-aggregation of chlorophyll-a derivatives inserting fluorinated phenylene linker
Authors: Hisahara Yuma , Nakano Takeo , Tamiaki Hitoshi 
Published: February 2025

Abstract
Chlorophyll-a derivatives inserting an ethynylene and fluorinated phenylene linker between the chlorin π-skeleton and hydroxymethyl group were prepared as chlorosomal chlorophyll models. The self-aggregation of these derivatives was investigated by means of electronic absorption and circular dichroism spectroscopy. The spectroscopic results revealed that the electronic interactions between the chlorin core and the peripheral fluorinated aromatic π-systems had a large effect on the optical properties of the synthetic chlorophyll-a derivative self-assemblies.
Executive Summary: State-of-the-Art Review: Complexities in Cardiac Implantable Electronic Device Infections: A Contemporary Practical Approach
Authors: Chesdachai Supavit , Baddour Larry M , Tabaja Hussam , Madhavan Malini , Anavekar Nandan , Zwischenberger Brittany A , Erba Paola Anna , DeSimone Daniel C 
Published: February 2025

Abstract
Cardiac implantable electronic device infections (CIEDIs) present substantial challenges for infectious diseases specialists, encompassing diagnosis, management, and complex decision-making involving patients, families, and multidisciplinary teams. This review, guided by a common clinical case presentation encountered in daily practice, navigates through the diagnostic process, management strategies in unique scenarios, long-term follow-up, and critical discussions required for CIEDIs.
Microtomography to traditional dental radiograph: projecting 3-dimensional initial proximal caries lesion annotations for enhanced radiographic delineation
Authors: Gonzalez-Valenzuela Ricardo E , Vu Quoc T D , Mettes Pascal , Loos Bruno G , Marquering Henk , Berkhout Erwin 
Published: February 2025

Abstract

Objectives
This study was undertaken to generate high-quality radiographic annotations of initial proximal carious lesions based on micro-CT scans. Specifically, we projected manually and automatically acquired annotations of micro-CT scans onto corresponding traditional dental radiographs.

Methods
We utilized the Diagnostic Insights for Radiographic Early-caries with micro-CT (ACTA-DIRECT) dataset of manually annotated initial proximal carious lesions in micro-CT scans and radiographs, the former serving as reference-standard. Production of high-quality radiographic annotations entailed the following: (1) acquiring a reference-standard (for a semi-automated approach) or generating a fully automated micro-CT-based annotation (for a fully automated approach); (2) simulating the corresponding radiograph by projecting the micro-CT scan to find the suitable projection parameters; and (3) superimposing micro-CT-based caries annotations onto radiographs, using identical projection parameters. To evaluate the subsequent accuracy of the annotations on radiograph, we assessed the sensitivity, specificity, and International Caries Classification and Management System (ICCMS) staging of micro-CT-based automated annotations. Projection accuracy was qualitatively gauged.

Results
Micro-CT-based automated annotations outperformed conventional annotations achieving a sensitivity of 50% (95% CI: 42%-59%) compared to 42% (95% CI: 34%-51%) and specificity of 99% (95% CI: 96%-100%) compared to 92% (95% CI: 87%-94%). Among correctly identified micro-CT-based automated annotations, 94% (61/65) were also accurately classified; and 80% of micro-CT projections were ranked as suitably similar to corresponding radiographs.

Conclusions
Micro-CT imaging offers resource-rich depictions, enabling more accurate annotations than those achievable through conventional means. By projecting micro-CT-based annotations of initial proximal caries onto radiographs, some limitations of the conventional radiograph annotation process may be overcome.
Machine learning and metabolomics identify biomarkers associated with the disease extent of ulcerative colitis
Authors: Ge Changchang , Lu Yi , Shen Zhaofeng , Lu Yizhou , Liu Xiaojuan , Zhang Mengyuan , Liu Yijing , Shen Hong , Zhu Lei 
Published: February 2025

Abstract

Background and aims
Ulcerative colitis (UC) is a metabolism-related chronic intestinal inflammatory disease. Disease extent is a key parameter of UC. Using serum metabolic profiling to identify noninvasive biomarkers of disease extent may inform therapeutic decisions and risk stratification.

Methods
The orthogonal partial least squares–discriminant analysis (OPLS-DA) was performed to identify the metabolites. Least absolute shrinkage and selection operator regression, random forest-recursive feature elimination, and support vector machine-recursive feature elimination algorithms were used to screen metabolites. Five machine learning algorithms (eXtreme Gradient Boosting, K-NearestNeighbor, Naive Bayes, random forest [RF], and SVM) were used to construct the prediction model.

Results
A total of 220 differential metabolites between the patients with UC and healthy controls (HCs) were confirmed by the OPLS-DA model. Machine learning screened 8 essential metabolites for distinguishing patients with UC from HCs. A total of 23, 6, and 6 differential metabolites were obtained through machine learning between groups E1 and E2, E1 and E3, and E2 and E3. The RF model had a prediction accuracy of up to 100% in all 3 training sets. The serum levels of tridecanoic acid were significantly lower, and pelargonic acid was significantly higher in patients with extensive colitis than in the other groups. The serum level of asparaginyl valine in patients with rectal UC was significantly lower than that in the E2 and E3 groups.

Conclusions
Our findings revealed the metabolic landscape of UC and identified biomarkers for different disease extents, confirming the value of metabolites in predicting the occurrence and progression of UC.
Nonconventional dysplasia in patients with inflammatory bowel disease and colorectal adenocarcinoma: a case-cohort study
Authors: Urquhart Siri A , Pallipamu Namratha , Voruganti Hima Varsha , Baraskar Bhavana , Muddaloor Pratyusha , Sethi Arshia K , Redij Renisha , Aedma Keirthana , Gopalakrishnan Keerthy , Poigai Arunachalam Shivaram , Burger Kelli N , Mahoney Douglas W , Kassmeyer Blake A , Lennon Ryan J , Kisiel John B , Coelho-Prabhu Nayantara 
Published: February 2025

Abstract

Background and Aims
Patients with inflammatory bowel disease (IBD) face increased risk of colorectal cancer (CRC). While the natural history of conventional dysplastic precursor lesions has been well-studied, the neoplastic potential of recently described nonconventional (NC) IBD-associated colonic mucosal lesions is unclear. We aimed to assess the incidence of antecedent NC lesions in patients with IBD who developed CRC.

Methods
A case-cohort study was performed to include patients with a diagnosis of IBD with or without CRC who underwent at least 2 surveillance endoscopic procedures at our institution between 1/1/2007 and 5/31/2023. NC lesions included serrated change and indefinite for dysplasia. Detection rates pre- and post-introduction of high-definition (HD) surveillance colonoscopy were compared.

Results
In total, 87 patients with IBD and CRC and 200 patients with IBD without CRC were identified. Of the cases, a majority had ulcerative colitis (n = 52, 60%), most commonly with extensive involvement (n = 46, 89%). Conventional (hazard ratio [HR] 2.18, 95% confidence interval [CI] 1.34-3.52) and NC (HR 2.28, 95% CI 1.59-3.26) lesions were associated with increased risk of CRC. Conventional lesions in the post-HD era appeared to have a stronger association with CRC (HR 2.79, 95% CI 1.62-4.77) than NC lesions (HR 1.62, 95% CI 0.86-3.06).

Conclusions
Both conventional and NC lesions seem to be associated with increased risk of CRC. Conventional lesions are more strongly associated with CRC than NC lesions in the post-HD era, but misclassifications in the pre-HD era may have resulted in a biased increased risk estimate for NC lesions.
The role of cardiovascular multimodality imaging in the evaluation of Anderson–Fabry disease: from early diagnosis to therapy monitoring
Authors: Cameli Matteo , Pieroni Maurizio , Pastore Maria Concetta , Brucato Antonio , Castelletti Silvia , Crotti Lia , Dweck Marc , Frustaci Andrea , Gimelli Alessia , Klingel Karin , Kuchynka Petr , Kuusisto Johanna , Lazaros George , Mandoli Giulia Elena , Merlo Marco , Moon James , Muraru Denisa , Pantazis Antonis , Rigopoulos Angelos G , Ristic Arsen , Elif Sade Leyla , Sheppard Mary N , Tschöpe Carsten , Petersen Steffen E , Imazio Massimo , Bohbot Yohann , Cikes Maja , Garg Pankaj , Keenan Niall , Petrescu Aniela , Stankovic Ivan , Szabo Liliana , Uusitalo Valtteri 
Published: February 2025

Abstract
Anderson–Fabry disease (AFD) is a rare genetic disease with X-linked transmission characterized by a defect in the enzyme alpha-galactosidase A, which impairs glycosphingolipid metabolism and leads to an excessive storage of globotriaosylceramide (Gb3) within lysosomes. AFD involves renal, cardiac, vascular, and nervous systems and is mainly observed in male patients with onset in childhood, although cardiac manifestation is often shown in adults. AFD cardiomyopathy is caused by the accumulation of Gb3 within myocytes first showed by left ventricular hypertrophy and diastolic dysfunction, leading to restrictive cardiomyopathy and systolic heart failure with biventricular involvement. The diagnosis of AFD cardiomyopathy may be insidious in the first stages and requires accurate differential diagnosis with other cardiomyopathies with hypertrophic phenotype. However, it is fundamental to promptly initiate specific therapies that have shown promising results, particularly for early treatment. A careful integration between clinical evaluation, genetic tests, and cardiac imaging is required to diagnose AFD with cardiac involvement. Basic and advanced echocardiography, cardiac magnetic resonance, and nuclear imaging may offer pivotal information for early diagnosis (Graphical Abstract), and the management of these patients is often limited to centres with high expertise in the field. This clinical consensus statement, developed by experts from the European Society of Cardiology (ESC) Working Group on Myocardial and Pericardial Diseases and the European Association of Cardiovascular Imaging of the ESC, aims to provide practical advice for all clinicians regarding the use of multimodality imaging to simplify the diagnostic evaluation, prognostic stratification, and management of cardiac involvement in AFD.
Homemade stent shields: fortifying against coronary perforation in STEMI patients amidst intensive glycoprotein IIb/IIIa inhibitors—a case report
Authors: Hanif Muhammad Imran , Tariq Sheikh Minahil 
Published: February 2025

Abstract

Background
Coronary artery perforation (CAP), though rare, is a serious complication of percutaneous coronary intervention (PCI) that demands prompt, tailored management. Swift recognition and intervention are essential. Typically, a covered stent is used to seal the perforation and restore vessel integrity. However, if unavailable, a homemade covered stent can be crafted and deployed. The choice of treatment depends on bleeding severity, patient stability, and available resources.

Case summary
A 70-year-old male with a history of diabetes and hypertension presented with severe chest pain persisting for 7–8 h, radiating to the left arm and jaw, accompanied by diaphoresis and nausea. His baseline electrocardiogram revealed an extensive anterior wall myocardial infarction, prompting immediate transfer to the catheterization laboratory for primary PCI. During the procedure, an unfortunate complication of CAP leading to cardiac tamponade was successfully managed by pericardiocentesis. With the unavailability of commercial covered stents, an improvised covered stent was fashioned and deployed, effectively sealing the perforation despite ongoing administration of glycoprotein IIb/IIIa inhibitors and heparin which was initiated following acute stent thrombosis. The patient's haemodynamic status stabilized during hospitalization, and he was discharged in a stable condition.

Discussion
Our case is a pioneering instance of using a homemade stent with glycoprotein IIb/IIIa inhibitors and heparin in a STEMI patient with CAP. This novel approach was successful, demonstrating its feasibility and safety. Our experience suggests that improvised stenting techniques could be a viable option in complex coronary interventions, especially when commercial alternatives are unavailable. Further validation may broaden treatment options and improve outcomes in interventional cardiology.
The Heart Team Approach to Cardiac Arrest
Authors: Thevathasan Tharusan , Pöss Janine , Montisci Andrea , Schmack Bastian , Eghbalzadeh Kaveh , Ott Sascha , Tavazzi Guido , Schrage Benedikt , D’Ettore Nicoletta , Pappalardo Federico 
Published: February 2025

Abstract
Cardiac arrest is a critical emergency in cardiovascular medicine, requiring rapid, multidisciplinary interventions to enhance patient survival and neurological outcomes. This review explores the unique challenges of managing out-of-hospital and in-hospital cardiac arrest, with a focus on mechanical circulatory support and extracorporeal cardiopulmonary resuscitation for selected patients. While out-of-hospital cardiac arrest management should prioritize rapid transport to specialized centers, in-hospital cardiac arrest may allow for immediate, patient-tailored interventions. Post-cardiac arrest syndrome adds complexity, often requiring nuanced mechanical circulatory support escalation and weaning. Standardized protocols, ethical considerations, and further research are essential to refine patient selection and improve outcomes, ultimately advancing cardiac arrest care.
Digest: Clinal variation in plant traits is shaped by plastic and evolutionary responses to water regimes and herbivory
Authors: Hsu Gen-Chang 
Published: February 2025

Abstract
How do water regime and herbivory shape phenotypic variation in plants along environmental gradients? Using a multifactorial field common garden approach, Jameel et al. (2024) showed that water availability and herbivore abundance influence the expression of foliar and reproductive traits in the perennial forb Boechera stricta. The concordance between phenotypic plasticity, phenotypic clines, and the direction of selection demonstrates the adaptive nature of plasticity in ecologically relevant traits. Furthermore, the experimental manipulations highlight which agents of selection drive the evolution of these traits.