bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human polyomaviruses JCPyV and MCPyV in urothelial cell carcinoma: a single institution experience
Authors: Klufah Faisal , Mobaraki Ghalib , Shi Shuai , Marcelissen Tom , Alharbi Raed A. , Mobarki Mousa , Almalki Shaia Saleh R. , van Roermund Joep , zur Hausen Axel , Samarska Iryna 
Published: December 2023

ObjectiveUrothelial cell carcinoma (UCC) is the most common type of urinary bladder. JCPyV and BKPyV have been detected in the urine and tissue of urothelial cell carcinomas (UCC) in immunocompetent patients. Here, we investigated the presence of several HPyVs in UCC samples using diverse molecular techniques to study the prevalence of HPyVs in UCC.MethodsA large single-institution database of urine cytology specimens (UCS; n = 22.867 UCS) has previously been searched for decoy cells (n = 30), suggesting polyomavirus infection. The available urine sediments and formalin-fixed paraffin-embedded (FFPE) tissue samples of UCC patients were tested for the presence of JCPyV-LTAg expression by immunohistochemistry (IHC) labeled with SV40-LTAg antibody (clone: PAb416) and subsequent PCR followed by sequencing. In addition, the presence of the oncogenic Merkel cell polyomavirus (MCPyV) and the presence of human polyomavirus 6 (HPyV6) and 7 (HPyV7) DNA were tested with DNA PCR or IHC.ResultsOf the 30 patients harboring decoy cells, 14 were diagnosed with UCC of the urinary bladder (14/30; 46.6%) before presenting with decoy cells in the urine. The SV40-LTAg IHC was positive in all 14 UCC urine sediments and negative in the FFPE tissues. JCPyV-DNA was identified in all five available UCS and in three FFPE samples of UCC (three of 14; 21.4%). Two UCC cases were positive for MCPyV-DNA (two of 14; 14.3%), and one of them showed protein expression by IHC (one of 14; 7.1%). All specimens were HPyV6 and HPyV7 negative.ConclusionOur findings show the presence of JCPyV in the urine and UCC of immunocompetent patients. Moreover, MCPyV was detected in two UCC cases. In total, five UCC cases showed the presence of either JCPyV or MCPyV. The evidence here supports the hypothesis that these viruses might sporadically be associated with UCC. Further studies are needed to confirm the relevance of JCPyV or MCPyV as a possible risk factor for UCC development.
Comparison of the effects of BDNF/TRKB signalling on metabolic biomarkers in the liver of sedentary and trained rats with normal and knockout BDNF genotypes
Authors: Grzelak Norbert , Kaczmarek Dominik , Mrówczyński Włodzimierz 
Published: December 2023

Introduction: The effect of brain-derived neurotrophic factor (BDNF) on the modulation of metabolic processes in the liver is poorly understood. Therefore, the aim of this study was to investigate whether hepatic concentrations or activities of metabolic biomarkers depend on altered BDNF/TrkB content in the liver, resulting from different BDNF genotypes of rats. In addition, it was assessed whether 5-week moderate endurance training modifies the levels of BDNF/Trk-B signaling and studied hepatic markers.Methods: Experiments were performed on wild-type and heterozygous BDNF knockout (HET, SD-Bdnf) rats, which were divided into four groups: control with normal genotype (Bdnf+/+), control with BDNF knockout genotype (Bdnf+/−), trained with normal genotype (Bdnf+/+T) and trained with BDNF knockout genotype (Bdnf +/−T). BDNF/TrkB concentrations as well as selected metabolic biomarkers including lipids—total cholesterol (CHOL), low-density lipoprotein (LDL), triglycerides (TG); enzymes—alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP); hormones—insulin (INS) and leptin (LEPT) as well as interleukin-6 (IL-6) as regeneration indicator were measured directly in liver homogenates.Results and Discussion: The study showed that Bdnf+/− rats exhibited reduced BDNF/TrkB signaling (BDNF, p &lt; 0.0001; Trk-B, p = 0.0005), altered lipid levels (CHOL, p &lt; 0.0001; LDL, p &lt; 0.0001; TG, p = 0.0006) and reduced hepatic ALAT (p = 0.0004) and GGT (p &lt; 0.0001) activity, which may contribute to hepatic steatosis and obesity, as well as indicate impairment of specific metabolic pathways in the liver. Interestingly, endurance training did not alter hepatic BDNF and TrkB content, but improved ALAT (p = 0.0366) and ASAT (p = 0.0191) activities and increased hepatic IL-6 (p = 0.0422) levels in Bdnf +/− rats, suggesting enhanced liver regeneration in animals with BDNF allele loss.
Genome-wide identification of the C2H2 zinc finger gene family and expression analysis under salt stress in sweetpotato
Authors: Du Taifeng , Zhou Yuanyuan , Qin Zhen , Li Aixian , Wang Qingmei , Li Zongyun , Hou Fuyun , Zhang Liming 
Published: December 2023

IntroductionThe higher plant transcription factor C2H2 zinc finger protein (C2H2-ZFP) is essential for plant growth, development, and stress response. There are limited studies on C2H2-ZFP genes in sweetpotato, despite a substantial number of C2H2-ZFP genes having been systematically found in plants.MethodsIn this work, 178 C2H2-ZFP genes were found in sweetpotato, distributed randomly on 15 chromosomes, and given new names according to where they were located. These members of the zinc finger gene family are separated into six branches, as shown by the phylogenetic tree. 24 tandem repeats of IbZFP genes and 46 fragment repeats were identified, and a homology study revealed that IbZFP genes linked more regions with wild relative species of sweetpotato as well as rhizome plants like potato and cassava. And we analyzed the expression patterns of IbZFP genes during the early development of sweetpotato storage roots (SRs) and salt stress using transcriptome data, and identified 44 IbZFP genes that exhibited differences in expression levels during the early expansion of sweetpotato SRs in different varieties, and 92 IbZFP genes that exhibited differences in expression levels under salt stress in salt tolerant and salt sensitive sweetpotato varieties. Additionally, we cloned six IbZFP genes in sweetpotato and analyzed their expression patterns in different tissues, their expression patterns under abiotic stress and hormone treatment, and subcellular localization.Results and discussionThe results showed that the IbZFP genes had tissue specificity in sweetpotato and were induced to varying degrees by drought and salt stress. ABA and GA3 treatments also affected the expression of the IbZFP genes. We selected IbZFP105, which showed significant differences in expression levels under salt stress and ABA treatment, to be heterologously expressed in Arabidopsis thaliana. We found that IbZFP105 OE lines exhibited higher tolerance to salt stress and ABA stress. This indicates that IbZFP105 can enhance the salt tolerance of plants. These results systematically identified the evolution and expression patterns of members of the C2H2-ZFP gene family in sweetpotato, providing a theoretical basis for studying the role of IbZFP genes in the development of sweetpotato SRs and in resistance to stress.
Development and validation of the Chinese coaches’ interpersonal style scale
Authors: Su Wen , Zhao Daliang 
Published: December 2023

PurposeCoaches’ behaviors and coaching styles play a critical role in influencing athletes’ psychological experiences and performance. According to the self-determination theory (SDT), coaches’ interpersonal behaviors are commonly categorized as autonomy-supportive and controlling. Due to less focus on the unique behaviors of Chinese coaches, this study incorporated coaches’ parental care for athletes, referred to as paternalistic benevolence, in their interpersonal styles in the context of the Chinese culture.MethodsExploratory factor analyses were used in studies 1 and 2 to find items associated with benevolent coaching behaviors and items to create the Chinese Coaches’ Interpersonal Style Scale. Study 3 used the constructed scale, as well as the Subjective Vitality Scale and Athlete Burnout Questionnaire, with a sample of athletes to examine scale reliability. The 15-item Chinese Coaches’ Interpersonal Style Scale contained three dimensions: benevolent, autonomy-supportive, and controlling coaching styles.ResultsThe findings showed that: (1) benevolent coaching behaviors held significant explanatory weight in the Chinese cultural context; (2) controlling and autonomy-supportive coaching styles were culturally congruent among both Eastern and Western athletes; and (3) benevolent and autonomy-supportive coaching behaviors positively impacted athletes, whereas controlling coaching behaviors had a negative impact.ConclusionThe measure showed strong validity and reliability, making it useful for future practice and research on the interpersonal style of Chinese coaches.
Exercise intervention to reduce mobile phone addiction in adolescents: a systematic review and meta-analysis of randomized controlled trials
Authors: Li Zuxian , Xia Xue , Sun Qilong , Li Yansong 
Published: December 2023

BackgroundThe growing problem of adolescent mobile phone addiction has attracted significant attention, underscoring the importance of identifying approaches to address it. Exercise has been found to reduce adolescent mobile phone addiction; however, its mechanism remains unclear. This review aims to elucidate the potential moderating factors between exercise and mobile phone addiction based on previous studies to provide a reference for adolescents to effectively participate in exercise to reduce mobile phone addiction.MethodsArticles were searched in the CNKI, Web of Science, Scopus, Cochrane, PsycINFO, and PubMed databases according to the inclusion criteria and followed the Preferred Reporting Items for Systematic Assessment and Meta-analysis (PRISMA). The quality of the literature was assessed by two independent reviewers using the Cochrane Collaboration Risk of Bias tool for methodological quality assessment. Meta-analysis was performed using Stata 15.1 software for Meta-analysis, standardized mean difference (SMD) was combined using a random effects model, and subgroup analysis was used to explore heterogeneity.ResultsA total of 12 studies, 17 samples, and 861 subjects were included in the meta-analysis, and all studies were randomized controlled trials. The findings revealed that the exercise intervention significantly reduced mobile phone addiction in adolescents (SMD = −3.11; 95% CI: −3.91, −2.30; p &lt; 0.001). The intervention effect was moderated by multiple variables, such as the measurement tools, exercise intervention types, cycles, frequency, and duration of a single exercise intervention.ConclusionOur findings suggest that exercise could serve as an effective strategy for preventing or ameliorating mobile phone addiction in adolescents. Based on the results of this study, we encourage mobile phone-addicted adolescents to engage in a single exercise using a mix of skills for 30–60 min three or more times weekly for more than eight consecutive weeks.
Clinical features and comorbidity in very early-onset schizophrenia: a systematic review
Authors: Di Luzio Michelangelo , Pontillo Maria , Villa Marianna , Attardi Anna Gaia , Bellantoni Domenica , Di Vincenzo Cristina , Vicari Stefano 
Published: December 2023

BackgroundVery early-onset schizophrenia (VEOS) is a form of schizophrenia that manifests before the age of 13 years and is characterized by the presence of positive, negative, and disorganized symptoms. The condition is exceptionally rare and, to date, limited studies have been conducted, resulting in incomplete information about its clinical features.MethodsThe present study involves a systematic review of the existing literature regarding the clinical features and comorbidities of VEOS.ResultsThe first search retrieved 384 studies. Of these, 366 were removed following the application of exclusion criteria, resulting in 18 studies for the final set.ConclusionThe results highlight that VEOS shares similarities with early-onset and adult-onset schizophrenia but also exhibits distinct and recognizable characteristics, including a more severe clinical profile (particularly in females), increased visual hallucinations, and high comorbidities with neurodevelopmental disorders. These findings may support clinicians in formulating early diagnoses and developing effective treatment strategies for pediatric and adolescent patients with psychosis.
Public preferences for online medical consultations in China: a discrete choice experiment
Authors: Wang Pei , Huang Yuankai , Li Haotao , Xi Xiaoyu 
Published: December 2023

BackgroundOnline medical consultation (OMC) is significant to promote the utilization and accessibility of healthcare resources and save time on consultation. However, the usage and public acceptance rates of it are still low in China. Meanwhile, few studies have focused on consumers’ demand of OMC services. This study aims to identify attributes that influence users’ preference for OMC services, quantify the value of these characteristics, and compare their relative importance.MethodsA nationwide discrete choice experiment was conducted to survey Chinese residents’ preference choices for six attributes of OMC services. Conditional logit model and mixed logit model were used to analyze respondents’ preference. Willingness to pay and heterogeneity were estimated by the mixed logit model.ResultsA total of 856 respondents completed the study, and 668 questionnaires passed the consistency test. All of 6 attributes in the study were statistically significant except for “Doctor’s professional title – Associate Senior.” When choosing OMC services, respondents preferred to spend as little time and money as possible on a large online medical platform to consult a high-rated physician with a senior title from a well-known Grade-A tertiary hospital. Besides, respondents valued doctor’s evaluation score most and were willing to pay ¥107 to obtain the services of higher-scored doctors.ConclusionThe study measured Chinese residents’ preferences for six attributes of OMC and showed the heterogeneity of attributes among subgroups. Our findings suggested that OMC services providers should reduce the customers’ waiting time, improve the quality of services and enhance professional skills to meet the customers’ requirements. More research on preferences for OMC needs to be conducted in China, especially for key populations such as patients with chronic diseases.
Discrepancy in diagnoses of diabetes and prediabetes using fasting plasma glucose and glycosylated hemoglobin and the underdiagnosis by ICD-10 coding: data from a tertiary hospital in Thailand
Authors: Poorirerngpoom Napalai , Ganokroj Poranee , Vorayingyong Arnond , Rattananupong Thanapoom , Pusavat Jennifer , Supasiri Thanan 
Published: December 2023

BackgroundEarly detection of prediabetes and diabetes better prevents long-term health complications. FPG and HbA1c levels are some common laboratory tests utilized as tools to diagnose diabetes and prediabetes, but the agreement rate between these two diagnostic tests varies, which could lead to underdiagnosis and thus undertreatment. This study aimed to analyze the agreement rate between FPG and HbA1c, as well as the physicians’ accuracy of using these results to make a prediabetes or diabetes diagnosis through ICD-10 coding at a tertiary care hospital in Bangkok, Thailand.MethodsA cross-sectional descriptive study was conducted using secondary data collected in a tertiary hospital’s check-up clinic from August 16, 2019 to June 30, 2022 to study the prevalence and diagnosis of diabetes and prediabetes, determined through FPG and HbA1c laboratory results. We analyzed the two laboratory tests’ diagnosis agreement rate and the physicians’ accuracy of diagnosing diabetes and prediabetes in ICD-10 coding using the FPG and HbA1c results.ResultsAmong 8,024 asymptomatic participants, the period prevalence diagnosed through laboratory results was 5.8% for diabetes and 19.8% for prediabetes. Diabetes and prediabetes prevalence based on laboratory data differs from that of ICD-10 coding data. Specifically, 79.6% of diabetes patients and 32.3% of prediabetes patients were coded using the ICD-10 coding system. 4,094 individuals had both FPG and HbA1c data. The agreement rate for diagnosing diabetes and prediabetes between the two laboratory results is 89.5%, with Kappa statistics of 0.58. Using only one of the two laboratory results would have missed a substantial number of patients.ConclusionOur findings highlight screening test discrepancies and underdiagnosis issues that impede diagnostic accuracy enhancement and refined patient management strategies. Early diagnoses of prediabetes and diabetes, especially before symptoms arise, could increase health consciousness in individuals, thereby enabling the implementation of lifestyle modifications and prevention of serious health complications. We emphasize the importance of diagnosing these conditions using both FPG and HbA1c, along with subsequent accurate ICD-10 coding. Even though some hospitals lack certified HbA1c testing, we suggest enhancing the availability of HbA1c testing, which could benefit many people in Thailand.Clinical trial registration:https://www.thaiclinicaltrials.org, identifier [TCTR20230824003].
Barriers, enablers, benefits, and drawbacks to point-of-care testing: a survey of the general practice out-of-hours service in Scotland
Authors: Mills Sarah EE , Akbar SM Babar , Hernandez-Santiago Virginia 
Published: December 2023

Background
The general practice out-of-hours (GPOOH) service is under pressure to treat more patients in less time, while reducing referrals and minimising diagnostic errors. Point-of-care (POC) testing involves rapid clinical tests that can be used to generate results during the consultation, and has the potential to facilitate managing these competing demands safely.

Aim
To describe current availability of POC tests in GPOOH in Scotland, and identify barriers, enablers, benefits, and drawbacks to its use.

Design & setting
Cross-sectional mixed-methods study, which surveyed opinions of clinicians working in the GPOOH service in NHS Scotland.

Method
An electronic questionnaire was developed, designed, piloted, and distributed to clinicians, which had closed questions and areas for free text.

Results
In total, 142 responses were received. Urine dipstick testing (99.2%), pregnancy tests (98.5%), oxygen saturation (97.7%), and blood glucose testing (93.9%), were the only POC tests commonly available in GPOOH in NHS Scotland. There was strongest support for the provision of POC tests, particularly C-reactive protein (CRP; 79.4%), strep A (76.0%), and D-dimer (75.2%). Responders felt that POC tests would improve confidence (92.3%) and safety (89.8%) surrounding clinical decision making, improve patient satisfaction (80.6%), and reduce hospital and secondary care referrals (77.5%). Barriers to POC test use were availability of the test kits and machines (94.5%), training requirements on how to use the machine (71.1%) and interpret results (56.3%), and time to do the test (62.0%).

Conclusion
Few POC tests are in regular use in GPOOH in Scotland. GPOOH clinicians are supportive of using POC testing. They identified a number of benefits to its use, with very few drawbacks. Increased provision of POC testing in GPOOH in NHS Scotland should be considered urgently.
The Rancangan Bangun Alat Perawatan dan Pemberian Nutrisi Otomatis Pada Tanaman Pakcoy Hidroponik Berbasis IOT Menggunakan Fuzzy Logic Control
Authors: Rahmatullah Zahrul Ulum 
Published: December 2023

Abstrak - Pada era globalisasi saat ini kemajuan teknologi sudah berkembang pesat, Hasil perkembangan teknologi salah satunya penerapan IoT (Internet of Things) pada alat otomatis untuk membantu petani didaerah perkotaan yang mempunyai lahan yang sempit. hampir semua penduduk di kota memiliki masalah lahan yang sempit dikarenakan padat penduduk, oleh karena itu memanfaatkan informasi ini untuk membantu pekerjaan mereka.; Penelitian ini bertujuan membahas bagaimana cara membuat alat yang memudahkan para petani yang tinggal di perkotaan untuk merawat dan memberikan nutrisi secara otomatis pada tanaman hidroponik.; Pada penelitian ini merancang sebuah alat perawatan dan pemberian nutrisi otomatis pada tanaman pakcoy hidroponik berbasis IOT dengan metode fuzzy logic. sistem ini menggunakan mikrokotroler ESP 32 sebagai system pengendali dan monitoring rangkaian input dan output, IOT sebagai pemantau jalannya operasi, fuzzy logic sebagai penerapan penalaran masalah kompleks yang tidak jelas, yang dapat dikontrol oleh operator manusia secara efisien, kemudian alat ini akan dirancang 4 sensor yang dimana sensor ini akan mengendalikan pompa untuk merawat dan mengisi nutrisi pada Tanaman pakcoy Hidroponik.

Kata kunci : Hidroponik, Fuzzy logic, IOT, Sensor BH1750, DHT11, TDS Meter, HY – SRF05, Esp 32, Relay, LED.
Identificação de Áreas de Preservação Permanente (APP) de nascentes no município de Landri Sales – PI e sugestões de práticas para sua proteção
Authors: Da Silva Raynara Ferreira , De Sousa Moema Barbosa , Dos Santos Graziele Nunes Lopes 
Published: December 2023

Os recursos hídricos, principal fonte de consumo humano, vem sofrendo alteração ao longo do seu processo evolutivo devido sua exploração desenfreada, dentre esses sistemas naturais, tem se as nascentes, que dão origem a rios e riachos, no qual seu entorno é composto por Áreas de Preservação Permanente (APPs). Pensando nisso é fundamental realizar a identificação destes locais para análise quanto ao seu estado de conservação e fornecimento de informações para sua proteção e conservação. Nesse contexto, o objetivo deste trabalho foi identificar áreas de preservação permanente de nascentes de Landri Sales, Piauí, bem como, gerar sugestões de práticas eficientes para sua proteção. Para identificação das nascentes a equipe da Secretaria de Meio Ambiente e Recursos Hídricos de Landri Sales (SEMMA) se deslocou ao local que, de acordo com critérios técnicos analisados pela equipe da SEMMA, considerando a Lei Federal 12.651/12, classificou os sistemas naturais como Áreas de Preservação Permanente e com auxílio de GPS as coordenadas foram coletadas. Foram identificadas duas nascentes, uma no Sítio Caldeirão e outra no Riacho da Areia, uma com sinais de degradação por atividades antrópicas e outra em estado de preservação. Por fim, foram fornecidas informações sobre sugestões de medidas que irão minimizar os impactos ambientais ocasionadas nestas áreas.
PRISMA GUIDELINES FOR SYSTEMATIC AND SCOPING REVIEWS: CULTURAL MAPPING OF CREATIVE INDUSTRIES
Authors: Santos Luana , Sousa Ana Luísa , Teixeira Luís 
Published: December 2023

While policymakers have had a great interest in debating Cultural and Creative Industries (CCIs), there is a lack of literature presenting a systematic overview of existing relevant research regarding CCIs mapping. This paper discusses and compares PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for Systematic Review (SR) and PRISMA for Scoping Review (ScR) for synthesizing research on CCIs mapping. ‘A systematic review attempts to collate all empirical evidence that fits pre-specified eligibility criteria to answer a specific research question. It uses explicit, systematic methods that are selected to minimize bias, thus providing reliable findings from which conclusions can be drawn and decisions made’ (Liberati et al. 2009: 65). ScR ‘(…) follow a systematic approach to map evidence on a topic and identify main concepts, theories, sources, and knowledge gaps’ (Tricco et al. 2018: 467). After analyzing and applying the guidelines and further documentation on both protocols, the ScR revealed to be more suitable in a complex field with distinct terminologies, production contexts, methodologies and objectives. Since certain topics in SR become optional, ScR is easier to apply in fields in which methods are difficult to compare and quantify, such as the Arts and Humanities.