bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rho-Kinase Is Differentially Expressed in the Adipose Tissue of Rodent and Human in Response to Aging, Sex, and Acute Exercise
Authors: Muñoz Vitor Rosetto , Vieira Renan Fudoli Lins , Katashima Carlos Kiyoshi , Gaspar Rafael Calais , Lino Marsel , Trombeta Joice Cristina dos Santos , Duft Renata Garbellini , Azevêdo Macêdo Ana Paula , da Silva Adelino Sanchez Ramos , Ropelle Eduardo Rochete , de Moura Leandro Pereira , Cintra Dennys Esper , Chacon-Mikahil Mara Patricia Traina , Cavaglieri Cláudia Regina , Pauli José Rodrigo 
Published: January 2024

Abstract
White adipose tissue (WAT) controls energy storage, expenditure, and endocrine function. Rho-kinase (ROCK) is related to impaired thermogenesis, downregulation of preadipocyte differentiation, and adipokine production. Furthermore, WAT ROCK responds to metabolic stress from high-fat diets or diabetes. However, ROCK distribution in adipose depots and its response to aging and sex remain unclear. Thus, we aim to investigate ROCK function in adipose tissue of rodent and human in response to aging and sex. We observed specific differences in the ROCK1/2 distribution in inguinal WAT (ingWAT), perigonadal WAT (pgWAT), and brown adipose tissue of male and female rodents. However, ROCK2 expression was lower in female ingWAT compared with males, a fact that was not observed in the other depots. In the pgWAT and ingWAT of male and female rodents, ROCK activity increased during development. Moreover, middle-aged female rodents and humans showed downregulation in ROCK activity after acute physical exercise. Interestingly, ROCK levels were associated with several inflammatory markers both in rats and humans WAT (Nfkb1, Tnf, Il1b, Il6, and Mcp1). Induction of cell senescence by etoposide elevates ROCK activity in human preadipocytes; however, silencing ROCK1/2 demonstrates improvement in the inflammatory and cell senescence state. Using public databases, several pathways were strongly associated with ROCK modulation in WAT. In summary, WAT ROCK increases with development in association with inflammatory markers. Further, ROCK activity was attenuated by acute physical exercise, implicating it as a possible therapeutic target for metabolism improvement mediated by adipose tissue inflammatory state changes.
Preliminary Validation of the Acute Care Mobility Assessment as a Measure of Hospital Mobility
Authors: Brown Cynthia J , Loyd Christine , Richardson J Tyler , Boogaerts Garner , Zhang Yue , Kennedy Richard E 
Published: January 2024

Abstract

Background
Despite heightened interest, measurement of hospital mobility remains challenging. Available assessment tools lack patient input regarding level and frequency of hospital mobility. The purpose of this study was to validate a brief self-reported mobility assessment to measure out-of-bed activity during hospitalization.

Methods
We recruited cognitively intact hospitalized adults (age ≥ 65 years) who walked prior to admission, to wear an accelerometer for 24 hours, and to complete the Acute Care Mobility Assessment (ACMA), a self-report of mobility that ranges from bed rest to walking off the hospital unit in the prior 24 hours. For each mobility level from sitting in a chair to walking off the unit, patients reported the frequency of the activity and the need for help from another person or equipment. Spearman correlation coefficients were calculated using several scoring algorithms to compare ACMA to accelerometer data.

Results
Fifty-one patients (mean age 74.3 [standard deviation 6.2] years, 63% female, 39% Black) had complete data. Steps taken in 24 hours ranged from 10 to 2 831. Correlation analyses identified strong associations between ACMA scores and total steps, and moderate correlations with total time walking using all algorithms. However, the unweighted frequency count using the 3 ambulation levels only (walking in room, in hall, and off ward) had the highest correlation with total steps (r = 0.84; p &lt; .001) and total time walking (r = 0.66; p &lt; .001).

Conclusions
ACMA is a valid measure of mobility among cognitively intact hospitalized older adults. The ACMA may add value to our current armamentarium of tools by adding patient reports of hospital mobility.
Unpacking the cost of the lunchbox for Australian families: a secondary analysis
Authors: Manson Alexandra C , Johnson Brittany J , Wolfenden Luke , Sutherland Rachel , Golley Rebecca K 
Published: January 2024

Abstract
Ninety per cent of Australian school children bring a home-packed lunch to school, with 44% of the food consumed during school hours being unhealthy. Among other factors, cost is a key consideration for food provision; however, the costs to Australian families are not well understood. Therefore, we aimed to determine what families are currently paying for school lunchboxes in Australian primary schools and to examine associations between food costs and socio-demographic factors with dietary quality. An audit of local retail outlets was used to determine the food costs of lunchbox contents. Costs (AUD) were adjusted for inflation as of early 2023. The lunchboxes of 1026 children aged 4–12 years at 12 Catholic primary schools in New South Wales, Australia, were assessed at the start of the day, using photography assessment methods and a validated School Food Checklist. The mean cost of lunchbox contents was $4.48 AUD (SD 1.53), containing a mean energy of 2699 kJ (SD 859), with 37.3% (SD 23.9) of energy sourced from unhealthy foods. Multiple linear regression analyses found that the strongest predictors of higher lunchbox cost (P &lt; 0.05) were a higher proportion of energy from unhealthy foods (B = 0.016) and lower Socio-Economic Indexes for Areas (B = −0.178), when controlling for child socio-demographics. The results indicated that lunchbox food costs to Australian families are comparable to alternative school food service models in Australia and internationally. Results demonstrate the cost of food is not the only barrier to providing a healthy school lunchbox. Demonstrating a need for cost-considerate systematic interventions addressing food provision challenges and socio-economic disparities faced by families.
Regulating short-term rental platforms: the effects of local regulatory responses on Airbnb’s operations in Europe
Authors: Wessel Michael , Schmidt-Kessen Maria José , Hukal Philipp 
Published: January 2024

Abstract
Many digital platforms offer services that affect real-world socio-economic processes. One example is the impact of short-term rental platforms such as Airbnb or Wimdu on cities and neighborhoods. Because these platforms often operate in a regulatory void characterized by absent, unclear, or poorly enforced laws and regulations, local governments in affected cities have begun experimenting with a variety of instruments to regulate the operations of short-term rental platforms. In this paper, we report how such locally implemented regulatory responses have affected Airbnb’s operations across 13 European cities over the period from 2015 to 2019. Using a difference-in-difference specification with synthetic controls, we assess the impact of different regulatory responses by disaggregating them into motivations, actions, targets, and outcomes. We find that the effectiveness of regulatory responses differs by type of regulation (restricting or clarifying), type of host (professional or private), as well as the enforcement (with or without the cooperation of the platform operator). Through this work, we add to the ongoing debate on the regulation of digital platforms by presenting both empirical evidence as well as an analytical framework.
Biomolecular profiles of Arctic sea-ice diatoms highlight the role of under-ice light in cellular energy allocation
Authors: Duncan Rebecca J , Nielsen Daniel , Søreide Janne E , Varpe Øystein , Tobin Mark J , Pitusi Vanessa , Heraud Philip , Petrou Katherina 
Published: January 2024

Abstract
Arctic sea-ice diatoms fuel polar marine food webs as they emerge from winter darkness into spring. Through their photosynthetic activity they manufacture the nutrients and energy that underpin secondary production. Sea-ice diatom abundance and biomolecular composition vary in space and time. With climate change causing short-term extremes and long-term shifts in environmental conditions, understanding how and in what way diatoms adjust biomolecular stores with environmental perturbation is important to gain insight into future ecosystem energy production and nutrient transfer. Using synchrotron-based Fourier transform infrared microspectroscopy, we examined the biomolecular composition of five dominant sea-ice diatom taxa from landfast ice communities covering a range of under-ice light conditions during spring, in Svalbard, Norway. In all five taxa, we saw a doubling of lipid and fatty acid content when light transmitted to the ice–water interface was &gt;5% but &lt;15% (85%–95% attenuation through snow and ice). We determined a threshold around 15% light transmittance after which biomolecular synthesis plateaued, likely because of photoinhibitory effects, except for Navicula spp., which continued to accumulate lipids. Increasing under-ice light availability led to increased energy allocation towards carbohydrates, but this was secondary to lipid synthesis, whereas protein content remained stable. It is predicted that under-ice light availability will change in the Arctic, increasing because of sea-ice thinning and potentially decreasing with higher snowfall. Our findings show that the nutritional content of sea-ice diatoms is taxon-specific and linked to these changes, highlighting potential implications for future energy and nutrient supply for the polar marine food web.
Genotype-associated heritable rumen bacteria can be a stable microbiota passed to the offspring
Authors: Zang Xinwei , Sun Huizeng , Xue Mingyuan , Liang Shulin , Guan Le Luo , Liu Jianxin 
Published: January 2024

Abstract
Recent studies have reported that some rumen microbes are “heritable” (those have significant narrow sense heritability) and can significantly contribute to host phenotype variations. However, it is unknown if these heritable rumen bacteria can be passed to the next generation. In this study, the rumen bacteria from mother cows (sampled in 2016) and their offspring (sampled in 2019) were assessed to determine if vertical transmission occurred between the two generations. The analysis of relationship between host genotypes and heritable bacterial abundances showed that potential of five host genotypes can affect the relative abundances of two unclassified species level heritable bacteria (Pseudoscardovia and p-251-o5). The G allele of BTB-01532239 and A allele of ARS-BFGL-NGS-8960 were associated with a higher relative abundance of p-251-o5. The A allele of BTB-00740910 and BovineHD1300021786 and G allele of BovineHD1900005868 were associated with a higher relative abundance of Pseudoscardovia. The mother–offspring comparison revealed that the heritable rumen bacteria had higher compositional similarity than nonheritable bacteria between two generations, and the predicted heritable microbial functions had higher stability than those from nonheritable bacteria. These findings suggest that a high stability exists in heritable rumen bacteria, which could be passed to the next generation in dairy cows.
Cerebrospinal fluid concentrations of posaconazole in paediatric leukaemia patients
Authors: Körholz Katharina , Holterhus Malcolm , Gordon Kathrin , Müller-Ohrem Charlotte , Müller Carsten , Groll Andreas H 
Published: January 2024

Abstract

Background
Little is known about the distribution of posaconazole in brain tissue and CSF. We therefore analysed trough concentrations of posaconazole in paediatric leukaemia patients in non-inflamed CSF.

Patients and methods
The study included paediatric patients &lt;18 years of age with acute leukaemia in remission who underwent repeat therapeutic lumbar punctures as part of their anti-leukaemia treatment. CSF and blood were obtained 20–24 h after dosing, and posaconazole was measured by LC-MS/MS.

Results
Six patients (median age: 10 years; range, 6–14) with acute lymphatic (three) or acute myeloid (three) leukaemia were included who received posaconazole gastroresistant tablets at weight-banded doses (five) or the oral solution (one). In contrast to 14 control samples, posaconazole was detectable in all 11 samples of treated patients. CSF concentrations ranged from 8.3 to 42 ng/mL with a median CSF concentration of 13.6 ng/mL. Concurrent serum concentrations were between 965 and 5177 ng/mL with a median of 1716 ng/mL.

Conclusions
Trough concentrations of posaconazole in the CSF after systemic administration were low but detectable in all subjects. Concurrent serum concentrations were in the target range for prophylaxis and treatment in 100% and 90%, respectively.
A Quantile–Quantile Toolbox for Reference Intervals
Authors: Hawkins Douglas M , Esquivel Rianne N 
Published: January 2024

AbstractBackgroundParametric statistical methods are generally better than nonparametric, but require that data follow a known, usually normal, distribution. One important application is finding reference limits and detection limits. Parametric analyses yield better estimates and measures of their uncertainty than nonparametric approaches, which rely solely on a few extreme values. Some reference data follow normal distributions; some can be transformed to normal; some are normal or transformable to normal apart from a few extreme values; and detection and quantitation limits can lead to data censoring.MethodsA quantile–quantile (QQ) toolbox provides powerful general methodology for all these settings.ResultsQQ methodology leads to a family of simple methods for finding optimal power transformations, testing for normality before and after transformation, estimating reference limits, and constructing confidence intervals.ConclusionsThese parametric methods have a particular appeal to clinical laboratorians because, while statistically rigorous, they do not require specialized software or statistical expertise, but can be implemented even in spreadsheets. We conclude with an exploration of reference values for amyloid beta proteins associated with Alzheimer disease.
Unveiling the cell wall-targeting mechanisms and multifaceted virulence modulation by a eugenol glycoconjugate against Aspergillus fumigatus: insights from in vitro and in ovo studies
Authors: Gupta Lovely , Verma Shalini , Goswami Lakshmi , Kamboj Himanshu , Sen Pooja , Bhattacharya Asish K , Vijayaraghavan Pooja 
Published: January 2024

Abstract

Aim
The primary objective of this study was to elucidate the putative cell wall-associated targets of compound 6i, a glycoconjugate of eugenol, in Aspergillus fumigatus, while also evaluating its toxicity and assessing histopathologic alterations in the liver, heart, and kidney of compound 6i-treated embryos using an in ovo model.

Method
To achieve this aim, compound 6i was synthesized, and a series of biochemical assays were performed to determine its impact on the fungal cell wall. Additionally, quantitative real time-PCR and liquid chromatography–mass spectrometry/mass spectrometry analyses were conducted to investigate changes in gene and protein expression profiles associated with melanin biosynthesis, conidiation, siderophore production, transcriptional regulation of β-glucan biosynthesis, and calcineurin activity in A. fumigatus.

Results
The experimental findings revealed that compound 6i exhibited notable antifungal activity against A. fumigatus by perturbing cell wall integrity, hindering ergosterol, glucan, and chitin biosynthesis, and inhibiting catalase production. Moreover, relative gene expression and proteomic analyses demonstrated that compound 6i exerted both downregulatory and upregulatory effects on several crucial genes and proteins involved in the aforementioned fungal processes. Furthermore, increased expression of oxidative stress-related proteins was observed in the presence of compound 6i. Notably, the glycoconjugate of eugenol did not elicit cytotoxicity in the liver, heart, and kidney of chick embryos.

Conclusion
The current investigation elucidated the multifaceted mechanisms by which compound 6i exerts its antifungal effects against A. fumigatus, primarily through targeting cell wall components and signaling pathways. These findings underscore the potential of the eugenol glycoconjugate as a promising antifungal candidate, warranting further exploration and development for combating A. fumigatus infections.
Loss of the auxiliary α2δ1 voltage-sensitive calcium channel subunit impairs bone formation and anabolic responses to mechanical loading
Authors: Kelly Madison M , Sharma Karan , Wright Christian S , Yi Xin , Reyes Fernandez Perla C , Gegg Aaron T , Gorrell Taylor A , Noonan Megan L , Baghdady Ahmed , Sieger Jacob A , Dolphin Annette C , Warden Stuart J , Deosthale Padmini , Plotkin Lilian I , Sankar Uma , Hum Julia M , Robling Alexander G , Farach-Carson Mary C , Thompson William R 
Published: January 2024

Abstract
Voltage-sensitive calcium channels (VSCCs) influence bone structure and function, including anabolic responses to mechanical loading. While the pore-forming (α1) subunit of VSCCs allows Ca2+ influx, auxiliary subunits regulate the biophysical properties of the pore. The α2δ1 subunit influences gating kinetics of the α1 pore and enables mechanically induced signaling in osteocytes; however, the skeletal function of α2δ1 in vivo remains unknown. In this work, we examined the skeletal consequences of deleting Cacna2d1, the gene encoding α2δ1. Dual-energy X-ray absorptiometry and microcomputed tomography imaging demonstrated that deletion of α2δ1 diminished bone mineral content and density in both male and female C57BL/6 mice. Structural differences manifested in both trabecular and cortical bone for males, while the absence of α2δ1 affected only cortical bone in female mice. Deletion of α2δ1 impaired skeletal mechanical properties in both sexes, as measured by three-point bending to failure. While no changes in osteoblast number or activity were found for either sex, male mice displayed a significant increase in osteoclast number, accompanied by increased eroded bone surface and upregulation of genes that regulate osteoclast differentiation. Deletion of α2δ1 also rendered the skeleton insensitive to exogenous mechanical loading in males. While previous work demonstrates that VSCCs are essential for anabolic responses to mechanical loading, the mechanism by which these channels sense and respond to force remained unclear. Our data demonstrate that the α2δ1 auxiliary VSCC subunit functions to maintain baseline bone mass and strength through regulation of osteoclast activity and also provides skeletal mechanotransduction in male mice. These data reveal a molecular player in our understanding of the mechanisms by which VSCCs influence skeletal adaptation.
Rapamycin does not alter bone microarchitecture or material properties quality in young-adult and aged female C57BL/6 mice
Authors: Devine Connor C , Brown Kenna C , Paton Kat O , Heveran Chelsea M , Martin Stephen A 
Published: January 2024

Abstract
Advancing age is the strongest risk factor for osteoporosis and skeletal fragility. Rapamycin is an FDA-approved immunosuppressant that inhibits the mechanistic target of rapamycin (mTOR) complex, extends lifespan, and protects against aging-related diseases in multiple species; however, the impact of rapamycin on skeletal tissue is incompletely understood. We evaluated the effects of a short-term, low-dosage, interval rapamycin treatment on bone microarchitecture and strength in young-adult (3 mo old) and aged female (20 mo old) C57BL/6 mice. Rapamycin (2 mg/kg body mass) was administered via intraperitoneal injection 1×/5 d for a duration of 8 wk; this treatment regimen has been shown to induce geroprotective effects while minimizing the side effects associated with higher rapamycin dosages and/or more frequent or prolonged delivery schedules. Aged femurs exhibited lower cancellous bone mineral density, volume, trabecular connectivity density and number, higher trabecular thickness and spacing, and lower cortical thickness compared to young-adult mice. Rapamycin had no impact on assessed microCT parameters. Flexural testing of the femur revealed that both yield strength and ultimate strength were lower in aged mice compared to young-adult mice. There were no effects of rapamycin on these or other measures of bone biomechanics. Age, but not rapamycin, altered local and global measures of bone turnover. These data demonstrate that short-term, low-dosage interval rapamycin treatment does not negatively or positively impact the skeleton of young-adult and aged mice.
Gendered barriers and opportunities for scaling integrated pest management practices along the mango value chain in Kenya
Authors: Muriithi Beatrice , Franchini Rachael , Kolhoff Paige , Seekman Victoria , Grossman Lydia , Mulungu Kelvin , Kirscht Holger , Ledermann Samuel , Khamis Fathiya Mbarak 
Published: January 2024

Abstract
Mango (Mangifera indica L.) is an important source of food and income in Kenya, but production is hampered by the proliferation of invasive fruit flies (Bactrocera dorsalis). The International Centre of Insect Physiology and Ecology (icipe) and its partners have over the past 2 decades developed and disseminated an integrated pest management (IPM) package of interventions that effectively reduce fruit fly populations, but adoption is relatively low. In response to this low adoption, the authors conducted desk- and field-based qualitative and quantitative studies to better understand the gendered barriers and opportunities for scaling IPM practices along the mango value chain in Kenya. Twenty-four gender-disaggregated focus groups, 118 farmer surveys, and 63 key informant interviews with value chain actors were conducted. The results show that lack of access to IPM inputs, market constraints, and access to training are among the key barriers to and motivations for adopting IPM strategies in Embu County, Kenya.
Intestinal macrophages in pathogenesis and treatment of gut leakage: current strategies and future perspectives
Authors: Selvakumar Balachandar , Sekar Priyadharshini , Samsudin A Rani 
Published: January 2024

Abstract
Macrophages play key roles in tissue homeostasis, defense, disease, and repair. Macrophages are highly plastic and exhibit distinct functional phenotypes based on micro-environmental stimuli. In spite of several advancements in understanding macrophage biology and their different functional phenotypes in various physiological and pathological conditions, currently available treatment strategies targeting macrophages are limited. Macrophages' high plasticity and diverse functional roles—including tissue injury and wound healing mechanisms—mark them as potential targets to mine for efficient therapeutics to treat diseases. Despite mounting evidence on association of gut leakage with several extraintestinal diseases, there is no targeted standard therapy to treat gut leakage. Therefore, there is an urgent need to develop therapeutic strategies to treat this condition. Macrophages are the cells that play the largest role in interacting with the gut microbiota in the intestinal compartment and exert their intended functions in injury and repair mechanisms. In this review, we have summarized the current knowledge on the origins and phenotypes of macrophages. The specific role of macrophages in intestinal barrier function, their role in tissue repair mechanisms, and their association with gut microbiota are discussed. In addition, currently available therapies and the putative tissue repair mediators of macrophages for treating microbiota dysbiosis induced gut leakage are also discussed. The overall aim of this review is to convey the intense need to screen for microbiota induced macrophage-released prorepair mediators, which could lead to the identification of potential candidates that could be developed for treating the leaky gut and associated diseases.