bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of 1α,25‐dihydroxyvitamin D3‐membrane associated, rapid response steroid binding protein expression in mouse odontoblasts by 1α,25‐(OH)2D3
Authors: Teillaud Christophe , Nemere Ilka , Boukhobza Florine , Mathiot Claire , Conan Nicole , Oboeuf Martine , Hotton Dominique , MacDougall Mary , Berdal Ariane 
Published: November 2004

AbstractThe rapid, nongenomic effects of 1α,25‐dihydroxyvitamin D3 (1α,25‐(OH)2D3) have been related to a 1,25D3‐membrane associated, rapid response steroid binding protein or 1,25D3‐[MARRS]bp, with a molecular weight of 65 kDa, in several tissues and species. Currently, no information is available concerning the nongenomic responses to 1α,25‐(OH)2D3 in dental tissues. In order to investigate the expression of 1,25D3‐[MARRS]bp in dental cells, in the presence or absence of 1α,25‐(OH)2D3, we have used rabbit polyclonal antibodies directed against the N‐terminus of the 1,25D3‐[MARRS]bp (Ab099) that recognizes the 1α,25‐(OH)2D3 binding protein in chick intestinal basolateral membranes and a mouse odontoblast‐like cell line (MO6‐G3). Western blotting and flow cytometric analyses with Ab099 specifically detected 1,25D3‐[MARRS]bp in MO6‐G3 cells. Moreover, 1,25D3‐[MARRS]bp was up‐regulated, in vivo, in differentiated dental cells. Electron microscopic analysis confirmed the plasma membrane localization of this binding protein and also showed its intracellular presence. Incubation of MO6‐G3 cells with different doses of 1α,25‐(OH)2D3 for 36 h resulted in an inhibition of 1,25D3‐[MARRS]bp expression with a maximal effect at 50 nM steroid. In addition, the culture media of MO6‐G3 cells contains immunoreactive 1,25D3‐[MARRS]bp. Immunogold positive membrane vesicle‐like structures are present in the extracellular matrix of MO6‐G3 cells. Altogether, these results indicate that the 1,25D3‐[MARRS]bp expression in MO6‐G3 cells is modulated by 1α,25‐(OH)2D3. In conclusion, this 1α,25‐(OH)2D3 binding protein could play an important role in the rapid, nongenomic responses to 1α,25‐(OH)2D3 in dental cells. © 2004 Wiley‐Liss, Inc.
Specificity of transcriptional regulation by the zinc finger transcription factors Sp1, Sp3, and Egr‐1
Authors: Al‐Sarraj Alia , Day Regina M. , Thiel Gerald 
Published: November 2004

AbstractThe transcription factors Sp1, Sp3, and Egr‐1 bind with their zinc finger DNA‐binding domains to GC‐rich sequences in the regulatory regions of their target genes. The similarity of the DNA‐binding sites of Sp1, Sp3, and Egr‐1 has triggered the hypothesis that they compete for the same DNA‐binding site. We have investigated the specificity of transcriptional regulation by Sp1, Sp3, and Egr‐1 using dominant‐negative mutants that block the DNA‐binding site of Sp1, Sp3, or Egr‐1, respectively. The results show that constitutive transcription of Sp1 regulated reporter genes, containing Sp1 sites derived from the aldolase C and p21WAF1/Cip1 genes, or the long terminal repeat of HIV‐1, was impaired by dominant‐negative mutants of Sp1 and Sp3, but not by a dominant‐negative Egr‐1. Transcription mediated by Egr‐1 was induced by transfection of expression vectors encoding wild‐type or mutated Egr‐1 or by stimulation of the extracellular signal‐regulated protein kinase pathway via an inducible B‐Raf‐estrogen receptor fusion protein. In all cases transcription of Egr‐1‐regulated reporter genes, containing Egr‐1 binding sites derived from the Egr‐1 or the synapsin I gene was impaired by a dominant‐negative Egr‐1, but not by dominant‐negative Sp1 or Sp3 mutants. These results show that there are genuine Sp1/Sp3 or Egr‐1 controlled genes showing no cross‐regulation of Sp1/Sp3 and Egr‐1 through the same DNA‐binding site. This does not exclude the existence of composite Sp1/Sp3/Egr‐1 binding sites, where competition for a common DNA‐binding site occurs. © 2004 Wiley‐Liss, Inc.
In support of fatty acid synthase (FAS) as a metabolic oncogene: Extracellular acidosis acts in an epigenetic fashion activating FAS gene expression in cancer cells
Authors: Menendez Javier A. , Decker Josiah P. , Lupu Ruth 
Published: November 2004

AbstractRelatively little information exists on the ultimate molecular mechanisms by which the lipogenic enzyme Fatty Acid Synthase (FAS) is differentially overexpressed in a biologically aggressive subset of human malignancies. Since the microenvironment of solid tumors contains regions of poor oxygenation and high acidity, it has recently been suggested that cancer‐associated FAS is a novel metabolic oncogene conferring a selective growth advantage upon stresses such as hypoxia and/or low pH. Here, we performed transient transfection studies with a 178‐bp FAS promoter fragment harboring a complex Sterol Regulatory Element Binding Proteins (SREBP)‐binding site to evaluate whether extracellular low pH and/or hypoxia may act in an epigenetic fashion by inducing changes in the transcriptional activation of FAS gene in cancer cells. First, MCF‐7 breast cancer cells cultured in acidosis (pH 6.5), but not under hypoxia or in the presence of hypoxia mimetics, demonstrated a more than two‐fold increase in the transcriptional activity of FAS promoter‐reporter constructs compared with control cells grown under standard culture conditions (pH 7.4). Second, the up‐regulatory effect of extracellular acidosis on the transcriptional activation of FAS gene was not observed when the FAS promoter was truncated at the SREBP‐binding site. Third, MCF‐7 cells engineered to overexpress the Her‐2/neu (erbB‐2) oncogene exhibited a SREBP‐dependent activation of the FAS promoter‐reporter construct up to three‐fold higher than that found in wild‐type MCF‐7 cells, while extracellular acidosis resulted only in a marginal increase of Her‐2/neu‐promoted activation of FAS gene. This study reveals for the first time that extracellular acidosis can work in an epigenetic fashion by up‐regulating the transcriptional expression of FAS gene in breast cancer cells, a stimulatory effect that is equally mimicked by well‐characterized oncogenic stimuli such as Her‐2/neu. These findings, altogether, support the “metabolic oncogene” theory for FAS overexpression in cancer cells. © 2004 Wiley‐Liss, Inc.
Method and algorithm of obtaining the molecular intrinsic characteristic contours (MICCs) of organic molecules
Authors: Yang Zhong‐Zhi , Gong Li‐Dong , Zhao Dong‐Xia , Zhang Ming‐Bo 
Published: November 2004

AbstractThe molecular intrinsic characteristic contour (MICC) is defined as the set of all the classical turning points of electron movement in a molecule. Studies on the MICCs of some medium organic molecules, such as dimethylether, acetone, and some homologues of alkanes, alkenes, and alkynes, as well as the electron density distributions on the MICCs, are shown for the first time. Results show that the MICC is an intrinsic approach to shape and size of a molecule. Unlike the van der Waals hard‐sphere model, the MICC is a smooth contour, and it has a clear physical meaning. Detailed investigations on the cross‐sections of MICCs have provided a kind of important information about atomic size changing in the process of forming molecules. Studies on electron density distribution on the MICC not only provide a new insight into molecular shape, but also show that the electron density distribution on the boundary surface relates closely with molecular properties and reactivities. For the homologues of alkanes,R,Dmin, andDmax(the minimum and maximum of electron density on the MICC), all have very good linear relationships with minus of the molecular ionization potential. This work may serve as a basis for exploring a new reactivity indicator of chemical reactions and for studying molecular shape properties of large organic and biological molecules. © 2004 Wiley Periodicals, Inc. J Comput Chem 26: 35–47, 2005
Differential carnitine/acylcarnitine translocase expression defines distinct metabolic signatures in skeletal muscle cells
Authors: Peluso Gianfranco , Petillo Orsolina , Margarucci Sabrina , Grippo Pasquale , Melone Mariarosa Anna Beatrice , Tuccillo Franca , Calvani Menotti 
Published: November 2004

AbstractImport of acylcarnitine into mitochondrial matrix through carnitine/acylcarnitine‐translocase (CACT) is fundamental for lipid catabolism. To probe the effect of CACT down‐expression on lipid metabolism in muscle, human myocytes were stably transfected with CACT‐antisense construct. In presence of low concentration of palmitate, transfected cells showed decreased palmitate oxidation and acetyl‐carnitine content, increased palmitoyl‐carnitine level, and reduced insulin‐dependent decrease of fatty acylcarnitine‐to‐fatty acyl‐CoA ratio. The augmented palmitoyl‐carnitine synthesis, also in the presence of insulin, could be related to an altered regulation of carnitine‐palmitoyl‐transferase 1 (CPT 1) by malonyl‐CoA, whose synthesis is dependent by the availability of cytosolic acetyl‐groups. Indeed, all the described effects were completely overcome by CACT neo‐expression by recombinant adenovirus vector or by addition of acetyl‐carnitine to cultures. Acetyl‐carnitine effect was related to an increase of malonyl‐CoA and was abolished by down‐expression, via antisense RNA strategy, of acetyl‐CoA carboxylase‐β, the mitochondrial membrane enzyme involved in the direct CPT 1 inhibition via malonyl‐CoA synthesis. Thus, in our experimental model the modulation of CACT expression has consequences for CPT 1 activity, while the biologic effects of acetyl‐carnitine are not associated with a generic supply of energy compounds but to the anaplerotic property of the molecule. © 2004 Wiley‐Liss, Inc.
Gene marking of human neural stem/precursor cells using green fluorescent proteins
Authors: Navarro‐Galve Beatriz , Villa Ana , Bueno Carlos , Thompson Lachlan , Johansen Jens , Martínez‐Serrano Alberto 
Published: November 2004

AbstractBackgroundEx vivo gene therapy and cell replacement in the nervous system may provide therapeutic opportunities for neurodegenerative disorders. The development of optimal gene marking procedures for human neural stem cells (hNSCs) is crucial for the success of these strategies, in order to provide a correct understanding of the biology of transplanted cells.MethodshNSCs were modified to express various members of the green fluorescent protein family of proteins. Both DNA and retroviral expression vectors were used. Cells were analyzed for transgene expression under transient and stable expression schemes, and in the presence or absence of drug selection, by fluorescence microscopy, histochemistry, immunocytochemistry, immunoblotting, RT‐PCR and flow cytometry. Genetically marked cells were analyzed in vivo after intrastriatal transplantation in neonatal rats.ResultsUsing the same experimental procedures, we have compared Aequorea victoria enhanced green fluorescent protein (Av‐eGFP) and Renilla raniformis GFP (Rh‐GFP, h‐ from humanized) for the purpose of gene marking of hNSCs. Our findings revealed practical problems for the derivation of stable Av‐eGFP‐expressing hNSCs, whereas Rh‐GFP could be well expressed. In a second phase of the study, stable Rh‐GFP‐expressing clonal hNSCs were derived. Rh‐GFP did not interfere with the differentiation potential of the cells, and expression levels were identical between division and differentiation conditions. Thirdly, in vivo, we have confirmed the usefulness of Rh‐GFP for the study of the transplant performance of hNSCs, and demonstrated that Rh‐GFP does not interfere with multipotency and differentiation.ConclusionsSearching for suitable and useful reporter genes, we have found that Rh‐GFP works efficiently for the purpose of stable gene marking of hNSCs, and is highly useful in vivo. The nature, properties, and possible side effects of marker genes are discussed, since these are important parameters to consider in gene marking studies involving hNSCs. Copyright © 2004 John Wiley & Sons, Ltd.
Similar substituent effects in the oxidations of primary aliphatic alcohols with dichromates and halochromates of heterocyclic bases
Authors: Karunakaran C. , Suresh S. 
Published: November 2004

AbstractThe reaction constants for the oxidations of primary aliphatic alcohols with 12 dichromates and halochromates of heterocyclic bases do not differ significantly indicating operation of a common mechanism. © 2004 Wiley Periodicals, Inc. Int J Chem Kinet 37: 5–9, 2005
Additive effect of hydrocarbons on OH radical production in H2 oxidation
Authors: Hidaka Yoshiaki , Ohnishi Takaaki , Wakamatsu Hitoshi 
Published: November 2004

AbstractMixtures of hydrocarbons (methane, allene, propyne, propene, and propane)–H2–O2 highly diluted with argon were heated to a temperature ranging from 1200 to 1900 K behind reflected shock waves, and the additive effects of methane, allene, propyne, propene, and propane on OH radical production in H2 oxidation were studied by observing time‐resolved UV‐absorption (306.7 nm). It was found that, in H2 oxidation below 1500 K, the addition of these hydrocarbons prolonged the delay time of the onset of the rapid OH radical production. An analysis using reported kinetic modeling of C1–C4 oxidation gave valuable information for reactions between hydrocarbons and H, O atoms and OH radicals. © 2004 Wiley Periodicals, Inc. Int J Chem Kinet 37: 50–55, 2005
Multiphase Polymer Dispersions and Nanocomposites by Catalytic/Free Radical Emulsion Polymerization
Authors: Kolb Ludmila , Thomann Ralf , Mecking Stefan 
Published: November 2004

AbstractSummary: Free radical emulsion polymerization of styrene (S) or butyl acrylate (BA) in the presence of latices of linear polyethylene (PE) prepared by catalytic emulsion polymerization affords colloidally stable multiphase latices. Coagulation of a PE/PS latex affords nanocomposites composed of small PE phases dispersed in a PS matrix, as evidenced by the large supercoolings of PE crystallization (by DSC). TEM of PE/PBA latices indicates a PBA phase around the PE particles under the emulsion polymerization conditions investigated. Films formed from these dispersions exhibit homogeneously dispersed PE particles.Multiphase latices are obtained by free radical emulsion polymerization of butyl acrylate in the presence of latices of linear polyethylene (PE) prepared by catalytic emulsion polymerization.imageMultiphase latices are obtained by free radical emulsion polymerization of butyl acrylate in the presence of latices of linear polyethylene (PE) prepared by catalytic emulsion polymerization.
Synthesis and Properties of Highly Photoluminescent and Electrochemically Active Polymers Containing 2‐Pyrazoline Units in the Main Chain
Authors: Fang Qiang , Jiang Biao , Xu Bing , Cao Amin 
Published: November 2004

AbstractSummary: Two new polymers containing 2‐pyrazoline units in the main chain were synthesized for the first time by a Suzuki polycondensation between a 2‐pyrazoline monomer and aryl diboronic esters. The polymers showed high photoluminescence (PL) in both the solution and the solid state; quantum yields of PL in toluene were higher than 76%. Thermogravimetric analysis and cyclic voltammetry showed that the polymers had high thermal stability and good reversibility under electrochemical oxidation.New polymers (2 and 3) with strong photoluminescence and good reversibility under electrochemical oxidation were synthesized here.imageNew polymers (2 and 3) with strong photoluminescence and good reversibility under electrochemical oxidation were synthesized here.
Modification of Soy Protein for Wood Adhesives using Mussel Protein as a Model: The Influence of a Mercapto Group
Authors: Liu Yuan , Li Kaichang 
Published: November 2004

AbstractSummary: Mussel protein can serve as a strong and water‐resistant adhesive, but is expensive and not readily available. Soy protein is inexpensive, abundant, and readily available, but is a poor adhesive. Mussel protein contains a high amount of mercapto‐containing cysteine. This study revealed that increasing the free mercapto group content in soy protein could greatly increase the strength and water‐resistance of wood composites bonded with the modified soy protein.Preparation of the modified SPIs.imagePreparation of the modified SPIs.
Heat Shrinkable Behavior and Mechanical Response of a Low‐Density Polyethylene/Millable Polyurethane/Organoclay Ternary Nanocomposite
Authors: Mishra Joy K. , Kim Il , Ha Chang‐Sik 
Published: November 2004

AbstractSummary: A low‐density polyethylene (LDPE)/millable polyurethane (PU)/organoclay ternary nanocomposite was successfully prepared. The nanocomposites were characterized by X‐ray diffraction (XRD) and transmission electron microscopy (TEM). The nanocomposites, as evidenced by XRD, are intercalated. The heat shrinkable behavior of the nanocomposites, as well as their pristine counterparts, was studied. It was observed that heat shrinkability decreases with increased filler content. The tensile strength and the tensile modulus of the nanocomposites are higher than their pristine counterparts.The heat shrinkability of the unfilled LDPE/millable PU blend is highest and it decreases with increased nanofiller content.imageThe heat shrinkability of the unfilled LDPE/millable PU blend is highest and it decreases with increased nanofiller content.
Cover Picture: Macromol. Rapid Commun. 21/2004
Authors: Leung Man Fai , Zhu Junmin , Harris Frank W. , Li Pei 
Published: November 2004

Cover: A transmission electron microscope image of microgel particles that consist of well‐defined temperature‐sensitive poly(N‐isopropylacrylamide) cores with pH‐sensitive chitosan shells is shown. The microgels are obtained directly from aqueous graft copolymerization of N‐isopropylacrylamide and N,N‐methylenebisacrylamide from chitosan. The unique core‐shell nanostructure exhibits tuneable responses to pH and temperature.Further details can be found in the Communication by M. F. Leung, J. Zhu, F. W. Harris, and P. Li* on page 1819.
Medial dorsal superficial peroneal nerve studies in patients with polyneuropathy and normal sural responses
Authors: Kushnir Mark , Klein Colin , Kimiagar Yitzhak , Pollak Lea , Rabey Jose M. 
Published: November 2004

AbstractWe studied medial dorsal superficial peroneal (MDSP) nerves in 52 patients with clinical evidence of mild chronic sensorimotor polyneuropathy and normal sural nerve responses, in order to assess the diagnostic sensitivity and usefulness of MDSP nerve testing in electrodiagnostic practice. To determine the effect of age on MDSP nerve parameters, 98 normal subjects were also examined. Electrodiagnostic evaluation involved studies of motor nerve conduction in tibial, peroneal, and median nerves; sensory nerve conduction in sural, MDSP, median, and radial nerves; tibial and peroneal nerve F waves; H reflexes from the soleus muscles; and needle electromyography of gastrocnemius and abductor hallucis muscles. Among the patients, 49% had low‐amplitude sensory responses in MDSP nerves and 57% had either slowing of sensory conduction velocity or no sensory responses on proximal stimulation. MDSP nerve amplitude, tibial nerve motor velocity, and H reflexes were the most sensitive for detection of mild chronic symmetrical axonal sensorimotor polyneuropathy. MDSP nerve testing should be included in the routine electrodiagnostic evaluation of patients with suspected polyneuropathy and normal sural nerve responses. Muscle Nerve, 2005
Peripheral neuropathy in hepatitis‐related mixed cryoglobulinemia: Electrophysiologic follow‐up study
Authors: Ammendola Angelo , Sampaolo Simone , Ambrosone Lucia , Ammendola Eduardo , Ciccone Gianluca , Migliaresi Sergio , Di Iorio Giuseppe 
Published: November 2004

AbstractA retrospective study was performed on 27 patients with hepatitis C (HCV)–related mixed cryoglobulinemia (purpura, arthralgia, hepatitis, glomerulonephritis, peripheral neuropathy) to assess peripheral nerve involvement during follow‐up of up to 8 years. All patients had the same degree of organ/system involvement initially and were clinically evaluated at least annually. All 27 patients received steroids; 15 also received recombinant interferon‐α2b (rIFN‐α2b). At first examination, neurological signs and electrodiagnostic findings consistent with peripheral neuropathy were found in 20 (74%) and in 24 (88.8%) patients, respectively. Neurological evaluation and electrodiagnostic data at 3 and 8 years revealed worsening of neuropathy, whereas the other manifestations of mixed cryoglobulinemia (MC) were stable. At the last examination, clinical and electrodiagnostic signs of neuropathy were found in 25 patients (92.5%), occurring in 1 of 3 patients with normal initial findings, and worsened in 8. A more severe neuropathy was observed in 3 (25%) of the patients treated with prednisone alone and in 6 (40%) of the patients additionally treated with rIFN‐α2b. Our data confirm that in patients with HCV‐related MC, peripheral nerve involvement is frequent, is progressive, and does not seem to benefit by addition of rIFN‐α2b to steroid treatment. Muscle Nerve, 2005
Myotonia and muscle contractile properties in mice with SIX5 deficiency
Authors: Personius Kirkwood E. , Nautiyal Joyti , Reddy Sita 
Published: November 2004

AbstractMyotonic dystrophy (DM1) is an autosomal‐dominant multisystem disease characterized by progressive skeletal muscle weakness, myotonia, cataracts, cardiac arrhythmias, mild mental retardation, and endocrinopathies. Heterozygous loss of SIX5 in mice causes cataracts and cardiac conduction disease, and homozygous loss also leads to sterility and decreased testicular mass, reminiscent of DM1 in humans. The effect of SIX5 deficiency in muscle is unknown. In this study, we found that muscle contractile properties, electromyographic insertional activity, and muscle histology were normal in SIX5 deficient mice. The implications of these findings for the pathogenesis of DM1 are discussed. Muscle Nerve, 2004
The Journal of Pathology moves forward
Authors: Herrington C Simon , Theobald Jeremy J , Newton Simon D , Smart Martin A 
Published: November 2004

AbstractThe purpose of this editorial is to give an update on various issues relating to the running of the The Journal of Pathology. In particular, ethical issues are discussed, forthcoming changes to the manuscript submission, tracking, and peer‐review system are outlined, and guidance on the submission of illustrations for publication is provided. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Treatment of pediatric febrile neutropenia in the era of vancomycin‐resistant microbes
Authors: Kline Ronald M. , Baorto Elizabeth P. 
Published: November 2004

AbstractPurposeThe increasing frequency of Gm+ infections in febrile neutropenic (FN) patients has resulted in increased use of vancomycin (VN). Likely as a result, VN‐resistant Enterococcus (VRE) has become a significant concern in FN patients. We sought to understand how the emergence of VN resistant microbes has changed the antibiotic management of pediatric FN.MethodsA questionnaire was distributed by e‐mail to responsible investigators of the Children's Oncology Group.ResultsOne hundred and thirty responses were analyzed. Forty‐four percent initially used monotherapy, with 82% of those using ceftazidime. Twenty‐seven used VN with another agent, generally ceftazidime. After the emergence of VRE and VN‐resistant staphylococcus (VRS), monotherapy increased to 58%. Ceftazidime continued to be most frequently used. There was a 57% reduction in the use of VN with 88% of centers not currently using VN in their initial treatment of FN. Forty‐seven percent of the centers that continue to use VN have VRE, while 90% that have discontinued its use have VRE/VRS.ConclusionsOurs is the first study to survey current practices in the treatment of pediatric FN and to document changes in practice patterns due to emerging antibiotic resistance patterns. We demonstrate increased use of monotherapy for FN, and a 57% decrease in the use of VN. Local considerations influence antibiotic choices with a significant difference in VRE prevalence between those centers that continue to use VN as compared to those that have discontinued it. © 2004 Wiley‐Liss, Inc.