bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chondrogenic and Osteogenic Differentiation of Adipose Tissue‐Derived Stem Cells Isolated from GFP Transgenic Mice
Authors: Ogawa Rei , Mizuno Hiroshi , Watanabe Atsushi , Migita Makoto , Shimada Takashi , Hyakusoku Hiko 
Published: January 2005

Aim:  Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have previously demonstrated that bone marrow derived stromal cells (BSCs) differentiate into a variety of cell lineages both in vitro and in vivo. In the present study, we extend this approach to characterize adipose tissue derived stromal cells (ASCs), sometimes called processed lipoaspirate (PLA) cells.Methods:  ASCs were isolated from inguinal fat pads of GFP mice. After the primary culture in control medium, the cells were incubated in osteogenic and chondrogenic medium for 2 to 4 weeks, respectively. Osteogenic and chondrogenic differentiation were assessed by the special staining and RT‐PCR.Results:  ASCs incubated in osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. RT‐PCR analysis showed the Expression of osteocyte related genes. Positive staining cells for alcian blue and the expression of chodrocyte related genes were found in ASCs incubated in chondrogenic medium.Conclusions:  These findings suggest that ASCs derived from GFP transgenic mice have both osteochondrogenic potential in vitro. Since this cell population can be easily identified through fluorescence microscope, it may be an ideal source of ASCs for further experiments of stem cell biology and tissue engineering.
Expression of the FGF Family and EGR‐1 in a Wound Healing Model (Reorganized Small Intestine From Fetal Mouse as an in Vitro Wound Healing Model)
Authors: Yonekawa Hironobu , Murata Eiko , Fujita Keiko , Satomi Akira , Akita Masumi 
Published: January 2005

Aim:  In the present study, expression of members of the fibroblast growth factor (FGF) family and early growth response gene 1 (Egr‐1), known as a transcription factor, was analyzed by reverse transcription polymerase chain reaction (RT‐PCR).Methods:  After the mouse fetal small intestine was divided into single cells with collagenase‐disperse, the cells were cultured. After reorganized small intestine were resected with a knife, expression of mRNAs from FGF family and Egr‐1 was analyzed by RT‐PCR.Results:  The wound surface was covered with epithelial cells by 24 hours after resection. Among members of the FGF family, mRNAs from FGF‐1, ‐2, ‐5, and ‐7 were expressed. Egr‐1 was expressed as early as 15 minutes after resection. Egr‐1 is thought to be a transcription factor that induces the expression of FGF‐2, other than factors involving wound healing, such as PDGF and TNF‐a.Conclusions:  In the present study, it was determined that this structure is a potential model for studying the roles of transcription factor and growth factors expressed in association with damage and wound healing.
Mechanism of Impairment in Wound Healing of Electron Beam‐Irradiated Rat Skin
Authors: Sugiyama Kenji , Nakatsuji Eri , Ochiai Atsushi , Esumi Hiroyasu , Matsumura Yasuhiro 
Published: January 2005

Aim:  Preoperative radiotherapy for head and neck or colon cancer frequently prevents the following surgical operation, since effective dose of ionized radiation induces wound complication. To make the operation possible, causes for the impaired wound healing were clarified.Methods:  Thighs of F344 rats were irradiated with electron beam (30 Gy). Seven days after the irradiation, full‐thickness skin of the thigh was cut by punch biopsy (4‐mm diameter). Healing process was determined by measuring size of wound opening. Cytokeratin 14, smooth muscle actin (a marker of myofibroblasts), and integrin beta1 were detected by immunohistochemical analyses.Results:  The wounds in the nonirradiated rats healed almost completely on day 7, whereas repair of epidermis was impaired in the irradiated skin. Numbers of hair follicles and micro‐vessels decreased, and myofibroblasts were almost missing in the scar and dermis adjacent to the wounds of irradiated skins. Proliferation indexes of epidermal basal cells were much higher in irradiated skins than those in nonirradiated skins, however, these basal cells did not express cytokeratin 14, concomitant with the absence of integrin beta1 in basement membrane of the irradiated epidermis. On the other hand, apoptosis appeared in the dermal follicular cells of hair bulbs, 18 h after the irradiation. On day 3, structures of hair follicles were destructed.Conclusions:  Reepithelialization of wounds was blocked in the irradiated skin. Since epidermal basal cells are supplied from stem cells in the hair bulge during cutaneous wound healing, one of causes for the impaired wound healing may be insufficiency of epidermal stem cells.
Preventive Effect of Fibroblast Growth Factor and Hepatocyte Growth Factor on Ceramide‐Induced Dysfunction Human Small Intestinal Cells
Authors: Yamaguchi Ryo , Takami Yoshihiro , Iida Takuya , Shimazaki Syuij 
Published: January 2005

Purpose:  Gastrointestinal (GI) damages associated with multiple organ failure is a fatal complication. The injured function of GI tract causes malnutrition and the bacterial translocation. So the functional recovery of GI tract is quite important for treating such cases. In the present study, we investigated whether basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) could recover the injured functions of cultured intestinal cells.Method:  Human small intestinal cells were cultured in a 10% FCS added DMEM. The cellular damages were induced by the treatment with various concentrations of cell permeable C6‐ceramide (0–20 M). Growth factors (10 ng/ml bFGF or 50 ng/ml HGF) were added to the medium simultaneously or 2 days after the beginning of the ceramide treatment. After 4 days of the treatment, cell growth and apoptosis induction were investigated.Result and Conclusion:  Ceramide inhibited cell growth and induced apoptosis in a dose‐dependent manner. Both growth factors significantly improved the cell growth and reduced apoptosis. These effects were also observed when the growth factor treatment was delayed 2 days after the ceramide treatment. It was found that bFGF was more significant than HGF on these protective effects. It is suggested that growth factor treatment may be a useful therapeutic option to improve GI tract dysfunction.
General session III Moderated by Takehisa Iwai: Muscular Flap Treatment for Intractable Ulcer with Artificial Vessel Exposure After a Surgery for Thoracic Aortic Aneurysm
Authors: Yamauchi T , Kiyokawa K , Tai Y , Inoue Y , Kizuka Y. 
Published: January 2005

Aim:  To heal an exposed artificial vessel due to mediastinitis after the replacement of thoracic aortic aneurysm.Methods:  The exposed vessel is washed thoroughly, wrapped in a muscular flap, and a free skin graft is grafted over it to close.Results:  An 81‐year‐old male repeatedly had mediastinum after the replacement of aneurysm, and it was not cured with muscular fillings, and a rubor on the chest developed to a subcutaneous hematoma. The lesion was closed with a patch on the artificial vessel, the mediastinal abscess was washed, and the opening was closed by using latissimus dorsi muscular flap and split‐thickness skin graft. No recurrence has occurred for 3 years. A 69‐year‐old male developed mediastinitis after the replacement of thoracic arch aortic aneurysm, and the wound reopened and the vessel exposed. Because conservative treatment did not help, pectoralis major muscular flaps with the perforating branches of the internal thoracic artery and vein as the pedicle were elevated from the both sides, filled the mediastinal space and a split‐thickness skin graft was grafted. No recurrence has occurred for one year.Conclusions:  Artificial vessels that developed infections are usually removed. However, we had good results by washing the lesion and wrapping it with muscular flap. By considering the size of surgical invasion and high death rate associated with the re‐replacement of artificial vessel or the opening of omentum, utilization of muscular flap should be considered as the first choice treatment.
Wound Healing and Scar Formation on the Skin of the Chest Wall and the Lower Leg After Cardiac Bypass Surgery
Authors: Takahashi Hajime , Kurata Daisuke , Takeuchi Masaki , Sasaki Kenji , Nozaki Motohiro 
Published: January 2005

Introduction:  Many patients receiving heart surgery have extensive operation scars on the skin surface of the chest wall and lower leg. In some cases there are long lasting open wounds in the lower leg after vein harvesting. Some patients complain of pain and itching and we can recognize hypertrophic or extensive scars, which we have to treat.Case and result:  We treated 16 patients (11 men and 5 women) ranging in age from 47 to 86. They were receiving many drugs such as anticoagulants, thus wound healing was delayed. In these cases, we choose not to resect tissue but apply ointment to remove necrotic tissue, and prepare good granulation tissue for smooth epithelization. Then, we employ prophylactic measures against hypertrophic scar formation such as tape containing steroid, silicon gel sheets and skin tapes as well oral medicine (toranilast) for the same purpose. Because of they have had to continuously take many oral medicines, these patients often do not want to take additional oral medicine. This is one point of difference in the treatment of heart surgery patients and patients with other types of wounds.
Involvement of KGF in the Wound Healing Process of Tracheal Cartilage in Rat Longitudinal Tracheotomy Model
Authors: Abo Takafumi , Matumoto Keitarou , Yamayosi Takatomo , Nagayasu Takesi , Izumi Sinich , Hisikawa Yositaka , Kouji Takehiko 
Published: January 2005

Aim:  To address whether KGF (Keratinocyte Growth Factor) and KGFR (keratinocyte Growth Factor Receptor) expression is involved in the wound healing process of tracheal cartilage in rat longitudinal tracheotomy model. And to expose the new strategy to control the process utilizing KGF and its related compounds.Method:  In this study, we incised rat trachea longitudinally for three cartilage lings, and harvested trachea from rat on 0, 1, 3, 5, 7 days after operation. We made horizontal section and observe them with immunohistochemistry.Result:  The cartilage defect was filled with chondrocytes originated from the perichondrium. KGF expression was observed in chondrocytes, perichondrium and fibroblasts in injured area. The expression of KGF was upregulated immediately after the operation, reaching a peak at 3–5 days.Future approach:  Further examination on temporal and spatial pattern of KGF and KGFR expression. Quantitative analysis of the expression of KGF and KGFR in relation to cell kinetics. Attempts to manipulate the kinetics of wound healing using KGF and its related compounds.
General session IV Moderated by Yoka Yoshimura: A Retrospective Study of Vacuum‐Assisted Closure in Japan
Authors: Miyamura Suguru , Terashi Hiroto , Yanagi Hideyuki , Tsuji Yoriko , Nomura Tadashi , Nonomura Hideaki , Tahara Shinya 
Published: January 2005

Aim:  Vacuum‐assisted closure (VAC) system, a new method for wound treatment, has been widespread over the world. There are few reports about this treatment in Japan. The purpose of this report is to consider the usefulness of VAC treatment at our department.Methods:  A retrospective review of 24 patients with 27 chronic wounds, which have been treated with VAC, was presented. The following parameters were studied: age, gender, present illness, complications, duration of VAC treatment, and its result.Results:  Eight women and 16 men were presented. The median age was 66.1 years (range, 20–84 years). Eleven of 27 wounds were mediastinitis. Six were pressure ulcers, and nine were other complicated ulcers. Tweleve patients had diabetes mellitus, and nine had hypertension. The median duration of VAC treatment was 32.5 days. Tweleve wounds healed only with VAC, and nine healed with a combination of VAC and small operation. Six were halfway stopped a VAC treatment due to infection.Conclusions:  Our results suggest that VAC system is an alternative treatment for chronic wounds of elder patients with high risk.
Chemical Analysis of the Surface of Silicone Modified by Oxygen Plasma Treatment for Cell Adhesion
Authors: Yamada Tai , Ohsugi Ikuko , Inoue Yoshikazu , Mizuno Kiyoyuki , Okumoto Takayuki , Yoshimura Yohko 
Published: January 2005

Aim:  As we confirmed O2 plasma treatment is useful for cell adhesion and growth on silicone surface by the culture experiment, reported in the other paper (Ohsugi et al). We aimed to confirm the change of the surface of silicone by O2 plasma treatment from the material‐scientific view.Material:  All materials were cut from a medical grade silicone mammary implant as the same sized disks. Group 1 is silicone disks with no treatment on material's surface. Group 2 is silicone disks with surface modifications by O2 plasma treatment for 3 minutes. Group 3 is silicone disks with surface modifications by O2 plasma treatment for 10 minutes.Method:  We investigated each group by the scanning electron microscope (SEM), the atomic force microscope (AFM), and X‐ray photoelectron spectoroscopy (XPS).Result:  As SEM and AFM shows, after O2 plasma treatment for 3 minutes, an original inequality formation (10 μm in width) on a surface of silicone were made flat, and for 10 minutes a crack formation occurred.From investigation by XPS, after O2 plasma treatment for 3 minutes, the ratio of oxygen on the surface of silicone increased. This phenomenon was thought that hydrophilic functional groups (‐OH, ‐COOH, etc.) were grafted on the surface of silicone. We supposed this is the reason why cell adhesion and growth became more effective.
Effect of Oxygen Plasma Treatment for Cell Adhesion Properties on Silicone Surface
Authors: Ohsugi Ikuko , Yamada Tai , Inoue Yoshikazu , Mizuno Kiyoyuki , Okumoto Takayuki , Yoshimura Yohko 
Published: January 2005

Aim:  The silicone polymer is known to be useful and safety as a material of medical implants. But fundamentally its biocompatibility and biostability are questionable. Thus we try to make a hybrid‐type silicone implant coating by cell layers. At first, we aimed to confirm cell adhesion and growth on silicone surface.Material:  Group 1 is silicone implants for the augmentation rhinoplasty with no treatment. Group 2 is same type silicone implants with surface modifications by O2 plasma treatment (3 min). Group 3 is the group 2 implants with serum and plasma coating (20 min).Method:  We co‐cultured materials of each group with human fibroblast (2.4*104 cells/ml), for 17 hours, with 5%CO2, in the temperatures of 37 °C.Results:  Group 1; none of fibroblasts could take the surface of materials.Group 2; several fibroblasts were found on the surface of those.Group 3; much more fibroblasts were found than group 2This result means that the surface modification by O2 plasma treatment itself enables the cell adhesion and growth on silicone surface.
Comparison of Bacteria‐Retaining Ability of Wound Dressings
Authors: Tachi Masahiro , Hirabayashi Shinichi , Yonehara Yoshiyuki , Uchida Gentaro , Tohyama Takuya 
Published: January 2005

We studied the bacterial retention capacity of alginate and carboxymethylcellulose dressings, using an infected skin ulcer model on the backs of rats. Wound surfaces were inoculated with either Staphylococcus aureus or Pseudomonas aeruginosa at a concentration of 1.5 × 106 cfu/wound. Carboxymethylcellulose dressing, alginate dressing A and alginate dressing B were applied to the contaminated wounds for 12 hours. Each dressing was then divided into two piece. Total viable bacterial count within the dressing was calculated using one piece, and bacterial count released from the dressing into physiological saline was determined using the other piece, enabling bacterial retention rate to be calculated. Each dressing was tested on each of 10 wounds. Statistical analyses were performed using one‐way analysis of variance (ANOVA) for replicated measures combined with Duncan's Multiple Comparison Test. Carboxymethylcellulose dressing was most effective in its ability to retain both Staphylococcus aureus and Pseudomonas aeruginosa(p < 0.05).Conclusions:  The bacterial retaining ability of carboxymethylcellulose dressing was found to be significantly higher than that of alginate dressings in an infected animal wound model.
Chitosan Sponge with Photocrosslinkable Chitosan Hydrogel Stimulates Large and Impaired Wound Healing in Rats
Authors: Ishizuka Toshiaki , Ishihara Masayuki , Obara Kiyohaya 
Published: January 2005

Aim:  We have previously reported that a photocrosslinkable chitosan hydrogel (Az‐CH‐LA) has induced wound healing in diabetic mice. However, deep and large skin wounds may be not enough covered by Az‐CH‐LA only. Therefore, we evaluated whether chitosan sponge with Az‐CH‐LA accelerates large and impaired wound healing.Methods:  Full thickness‐square wounds (25 × 25 mm) were prepared on the upper back of twenty‐four male Sprague Dawley rats. Chitosan sponge and/or Az‐CH‐LA aqueous solution (30 mg/ml) was added onto the wound of each rat and was then irradiated with UV light. Thus, the following four experimental groups were studied: Group (G) 1: control, G2: Az‐CH‐LA solution only, G3: Chitosan sponge + 0.9% saline, or G4: Chitosan sponge + Az‐CH‐LA solution. The changes in wound area were measured at day 2, 4, 8, and 16. The skin including the wound was removed from each rat for histological examination.Results:  The wound area in G4 at day 2, 4, and 8 was significantly smaller than the other groups. The number of neovessels and the thickness of granulation tissue in G4 at day 2 and 4 were significantly higher than the other groups.Conclusions:  The chitosan sponge with Az‐CH‐LA has been found to be more effective in large and impaired wound healing compared with a Az‐CH‐LA solution only.
Liver Regeneration After Resection of Multiple Liver Cysts
Authors: Suehiro Taketoshi , Hirashita Teijirou , Mashino Koujirou , Sunmiyoshi Yasushi , Takeuchi Hideya , Aramaki Masanori , Watanabe Akihiro , Kanoh Tadashi 
Published: January 2005

Background:  A regenerative function is in one of the functions of liver, and if it is a normal liver, it will reproduce even to capacity almost before an operation within one year after excision. However, liver substance is pressed over the long period of time, and, as for liver cysts, a postoperative liver reproduction process is unknown. Comparison examination of the multiple liver cysts postoperative liver reproduction was carried out with after the operation.Patients:  It was aimed at one example of a multiple liver cysts operation and three metastatic live tumor (two extended left lobectomy, one right lobectomy) in this hospital. Liver volume was measured before an operation and 14 postoperative days CT, and comparison examination of the standard liver volume ratio called for from height weight was carried out.Results:  Before an operation of liver cysts and 14 postoperative day, liver volume was 151% 45 or 69% in 450 or 680 ml. Before an operation of control group and day 14 postoperative day liver volume was an average of 128% at 116 and 128 or 140% in 62, 72, 56, 71, and 49 or 68% at 618, 720, 532, 680, and 516 or 720 ml, respectively.Conclusion:  Multiple liver cysts postoperative liver reproduction was promoted compared with after the liver resection.
General session VI Moderated by Nobutoshi Andou: Lung Vitamin E Depletion Resulting from Burn and Smoke Inhalation in Sheep
Authors: Shimoda Katsumi , Soejima Kazutaka , Isago Tsukasa , Nozaki Motohiro , Traber Daniel L. 
Published: January 2005

Introduction:  Oxidants are involved in the pathogenesis of multiple trauma caused by burn and smoke inhalation. α‐ and γ‐tocopherol are major tissue antioxidants and their depletion reflects oxidant injury. We hypothesized that lung vitamin E levels in sheep would be depleted with multiple trauma.Methods:  Sheep (n = 31) were surgically prepared for chronic study, then received either a 40% body surface area third‐degree burn, 48 breaths of cotton smoke, a combination of these injuries (B&S) or were given sham injuries. All sheep were resuscitated with Ringer's lactate solution (4 ml/kg/%BSA burn) and mechanically ventilated. The sheep were sacrificed at various time intervals as indicated and vitamin E was measured (nmol/g) in snap‐frozen lung samples obtained at autopsy 48 h postinjury.Results:  Lungα‐tocopherol was significantly depressed in sheep with the combination injury, smoke alone and burn alone, respectively, compared to sham treated (p < 0.05). Lungγ‐tocopherol was significantly depressed in sheep with the combination injury compared to sham treated (p < 0.05). Neither smoke alone nor burn alone animals were statistically different from sham.Conclusion:  α‐tocopherol depletion may be a result of lipid peroxidation, while γ‐tocopherol depletion may reflect both lipid peroxidation and peroxynitrite damage (nitro‐γ‐tocopherol formation). These results suggest that animals receiving a combination burn and inhalation injury have undergone marked oxidative stress.
General session VII Moderated by Chifumi Yoshida: A Case of Ulcerative Colitis with Peristomal Pyoderma Gangrenosum
Authors: Ebata Tomoe 
Published: January 2005

Introduction:  Pyoderma gangrenosum is a skin disease that can result in progressive necrosis and formation of painful ulcers. This is to report on a case where the patient developed the disease around a stoma.Case:  A 40‐year‐old female patient developed ulcerative colitis (UC) when she was 25. At the age of 38, the exacerbation of UC required a colectomy and ileal pouch anal canal anastomosis (IACA), following which, a permanent ileostomy was constructed due to pouchitis. In April 2003, she developed redness around the stoma with pain, which resulted in the gradual formation of an ulcer. She was hospitalized with a diagnosis of peristomal pyoderma gangrenosum (PPG). After admission to the hospital, the dosage of predonine was increased to 30 mg/day, and dapsone was concomitantly administered. Clobetasol propionate and sulfadiazine silver were topically administered 1–4 times daily. The wound region adjacent to the stoma was protected with a gauze dressing. Although the gauze was changed each time, it was contaminated with fecal matter, the suffering thereby inflicted on the patient was too severe due to the associated smell and staining of her clothing. For this reason, she alternatively started wearing a stoma bag, which enabled periodic replacement once daily. Following a gradual improvement of the ulcer, she was discharged from the hospital two months after admission. Her recovery was confirmed four months after onset.Conclusion:  PPG is accompanied by pain, and treatment also involves suffering by the patient. We need to make the diagnosis and determine the treatment method while the disease is still in its early stages. The selection of the method of care should be made with consideration of the practical aspect of the patient's defecation control.