bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of septal cholinergic input to lateral entorhinal cortex is an early indicator of cognitive impairment
Authors: Ananth Mala R. , Gardus John D. , Huang Chuan , Palekar Nikhil , Slifstein Mark , Zaborszky Laszlo , Parsey Ramin V. , Talmage David A. , DeLorenzo Christine , Role Lorna W. 
Published: January 2024

Abstract

Although alterations to central cholinergic signaling are characteristic of late-stage cognitive deficits, the early temporal profile of these alterations and their relationship to more subtle changes in cognition are less clear. In a series of translational experiments in humans and mice, we evaluated whether changes to the cholinergic system were an early feature of pathological aging. Additional studies in mice assessed the effects of selective perturbations of cholinergic signaling on cognitive performance. We focus on the cholinergic innervation of the entorhinal cortex (EC), a region that is recognized to be susceptible early in aging and neurodegenerative disease. In human studies we recruited older adult volunteers that were physically healthy and without clinical diagnosis of cognitive impairment. Participants were divided based on their cognitive status during the intake session. Using positron emission tomography (PET) with a tracer specific for the vesicular acetylcholine transporter ([18F]VAT) we demonstrate that loss of cholinergic input to the EC is an early occurrence in cognitive impairment. These deficits are specific to the cholinergic circuit between the medial septum and vertical limb of the diagonal band (MS/vDB; CH1/2) to the EC. We further demonstrate impaired structural connectivity in the tracts between the MS/vDB and EC in cognitively impaired, older adults. Mouse experiments, designed to parallel the human studies, used high resolution terminal field imaging to compare normal aged mice with a genetically modified line with accumulation of amyloid beta plaques and spontaneous hyperphosphorylation of mouse tau. Across species we find that the integrity of cholinergic projections to the EC directly correlates with performance in EC-related object recognition memory tasks. We further establish the role of the MS/vDB regions of the cholinergic basal forebrain in object location memory by demonstrating that silencing EC-projecting cholinergic neurons in normal animals is sufficient to impair object recognition performance. Taken together we identify a role for acetylcholine in normal EC function and establish loss of cholinergic input to the EC as an early, conserved feature in age-related cognitive decline.
Operation of a solar power plant with dual-axis solar tracker
Authors: Mitrofanov S. V. , Baykasenov D. K. 
Published: January 2024

The paper evaluates the generation of electrical energy by a solar power plant equipped with a solar tracking system using the ASHRAE clear-sky method for calculating solar insolation. The mathematical algorithm uses the MathCad system with data export and analysis in Microsoft Excel. Collected over a month and the operation period in 2022–2023, data on electricity generation by solar stations characterised by an optimal constant angle of inclination of the solar panel and equipped with a solar tracking system were used. By taking into account the varying ambient temperature, dust content, and solar transmission by the solar panel coating, the given algorithm allowed electricity generation by a solar panel to be forecasted with an average absolute error ranging from 0.22 to 9.8. To determine solar insolation for a specific day and the geographical coordinates of the intended construction site of a solar power plant, a mathematical model was developed using MathCad software. The experimental and computational studies carried out on selected clear days, accounting for varying weather conditions, demonstrated the adequacy of this method and its applicability for forecasting electricity generation with different inclination angles of a solar panel. It was established that a solar power plant with a solar tracking system generated 37% more electricity per year than that with fixed solar panels. The ASHRAE Clear-Sky method for calculating solar insolation allows the amount of electricity generated for a specific region to be estimated with minimal input data. Further research will focus on defining and improving methods for forecasting electricity generation by a solar power plant on overcast days.
Development in desalination and wastewater treatment: state of the art challenges, role of solar energy, and recommendations
Authors: Khan Mohd Shariq , Ansari Khursheed B. , Fatima Atiya , Mesfer Mohammad K. Al , Danish Mohd , Tawfik Ahmed , Maheshwari Utkarsh 
Published: January 2024

Abstract

Water purification is crucial to ensure the availability of safe and clean water for protecting human health and helping in a sustainable environment. This review explicitly provides an overview of the major developments related to seawater desalination and wastewater treatments as a unit. Anticipating these traditional independent facilities will operate together in the future mainly for economic benefits. The advances in seawater desalination are primarily focused on the production of membranes using different types of nanoparticles (CeO2, UiO-66, CS-NPs) and carboxylated multi-walled CNTs into a polyamide that provides high salt rejection (as high as 90%) with excellent selectivity and permeability. Further, the use of nanoporous reduced graphene oxide (rGO) membranes provided 27.7–62.6% desalination rates and up to 96.8% salt rejection, along with protection against membrane fouling. Metal-organic frameworks (MOFs) and covalent organic frameworks (COFs) showed excellent rejection of divalent ions during desalination. It is anticipated that the use of solar energy for membrane and thermal desalination may be promising since it reduces the reliance on fossil energy and significantly minimizes greenhouse gas emissions (up to 94%). Solar energy can integrate with the reverse osmosis (RO) process bearing the cost of energy-intensive compressors, making the RO economically attractive.
Environmental Risk Factors in Autism Spectrum Disorder: A
Narrative Review
Authors: Yenkoyan Konstantin , Mkhitaryan Meri , Bjørklund Geir 
Published: January 2024

Abstract:
Existing evidence indicates that environmental factors might contribute up to
50% of the variance in autism spectrum disorder (ASD) risk. This structured narrative review
offers a comprehensive synthesis of current knowledge on environmental risk factors
in ASD, including evaluation of conflicting evidence, exploration of underlying
mechanisms, and suggestions for future research directions. Analysis of diverse epidemiological
investigations indicates that certain environmental factors, including advanced
parental age, preterm birth, delivery complications, and exposure to toxic metals, drugs,
air pollutants, and endocrine-disrupting chemicals, are linked to an increased ASD risk
through various mechanisms such as oxidative stress, inflammation, hypoxia, and its
consequences, changes in neurotransmitters, disruption of signaling pathways and some
others. On the other hand, pregnancy-related factors such as maternal diabetes, maternal
obesity, and caesarian section show a weaker association with ASD risk. At the same
time, other environmental factors, such as vaccination, maternal smoking, or alcohol consumption,
are not linked to the risk of ASD. Regarding nutritional elements data are inconclusive.
These findings highlight the significance of environmental factors in ASD
etiology and emphasize that more focused research is needed to target the risk factors of
ASD. Environmental interventions targeting modifiable risk factors might offer promising
avenues for ASD prevention and treatment.
The Role of Sgt1 in Methamphetamine/Hyperthermia-induced Necroptosis
Authors: Lu Shuang , Wang Lewen , Liao Lvshuang , Niu Shuliang , Xiong Kun , Yan Jie , Yan Weitao 
Published: January 2024

Introduction:
Methamphetamine (METH) is a synthetic drug widely abused
globally and can result in hyperthermia (HT) and psychiatric symptoms. Our previous
studies showed that heat shock protein 90 alpha (HSP90α) plays a vital role in
METH/HT-elicited neuronal necroptosis; however, the detailed mechanism of HSP90α
regulation remained obscure.

Methods:
Herein, we demonstrated a function of the suppressor of G-two allele of SKP1
(Sgt1) in METH/HT-induced necroptosis. Sgt1 was mainly expressed in neurons, co-located
with HSP90α, and increased in rat striatum after METH treatment. METH/HT injury
triggered necroptosis and increased Sgt1 expression in PC-12 cells.

Results:
Data from computer simulations indicated that Sgt1 might interact with
HSP90α. Geldanamycin (GA), the specific inhibitor of HSP90α, attenuated the interaction
between Sgt1 and HSP90α. Knockdown of Sgt1 expression did not affect the expression
level of HSP90α. Still, it inhibited the expression of receptor-interacting protein 3
(RIP3), mixed lineage kinase domain-like protein (MLKL), p-RIP3, and p-MLKL, as
well as necroptosis induced by METH/HT injury.

Conclusion:
In conclusion, Sgt1 may regulate the expression of RIP3, p-RIP3, MLKL,
and p-MLKL by assisting HSP90α in affecting the METH/HT-induced necroptotic cell
death.
Immunohistochemical Expression of the SERPINA3 Protein in Uterine
Fibroids
Authors: de Mezer Mateusz , Markowska Anna , Markowska Janina , Krzyżaniak Monika , Grabarek Beniamin Oskar , Pokusa Filip , Żurawski Jakub 
Published: January 2024

Background:
SERPINA3 (α-1-antichymotrypsin, AACT, ACT) is produced by the
liver and released into plasma in an anti-inflammatory response and plays a role as a modulator
of extracellular matrix (ECM) by inhibiting serine proteases. Numerous studies proved an increased
level of SERPINA3 in many types of cancer, which could be linked to SERPINA3’s
anti-apoptotic function.

Aim:
In the context of progressive ECM fibrosis during the development of uterine fibroids,
which are one of the most common hypertrophic changes within the uterus, it is interesting to
describe the level of SERPINA3 protein in this type of lesion and the surrounding tissues.

Methods:
We used immunohistochemical staining of the SERPINA3 protein and compared the
intensity of the signal between the myoma tissue and the surrounding normal tissue.

Results:
We showed a surprising reduction in the amount of the SERPINA3 protein within uterine
fibroids compared to surrounding tissues.

Conclusion:
This observation sheds new light on the role of this protein in the formation of proliferative
changes and suggests that understanding the mechanism of its action may become the
basis for the development of new diagnostic and therapeutic tools.
Amiodarone Advances the Apoptosis of Cardiomyocytes by Repressing Sigmar1 Expression and Blocking KCNH2-related Potassium Channels
Authors: Liang Huiqing , Li Huixian , Li Fangjiang , Xiong Xiaobo , Gao Yang 
Published: January 2024

Background:
Heart failure (HF) is the ultimate transformation result of
various cardiovascular diseases. Mitochondria-mediated cardiomyocyte apoptosis has
been uncovered to be associated with this disorder.

Objective:
This study mainly delves into the mechanism of the anti-arrhythmic drug
amiodarone on mitochondrial toxicity of cardiomyocytes.

Methods:
The viability of H9c2 cells treated with amiodarone at 0.5, 1, 2, 3, and 4 μM
was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
assay, and Sigmar1 expression was examined by quantitative real-time PCR (qRTPCR).
After transfection, the viability, apoptosis, reactive oxygen species (ROS) level,
mitochondrial membrane potential (MMP), and potassium voltage-gated channel
subfamily H member 2 (KCNH2) expression in H9c2 cells were assessed by MTT, flow
cytometry, ROS assay kit, mitochondria staining kit, and Western blot.

Results:
Amiodarone at 1-4 μM notably weakened H9c2 cell viability with IC50 value of
2.62 ± 0.43 μM. Amiodarone at 0.5-4 μM also evidently suppressed the Sigmar1 level
in H9c2 cells. Amiodarone repressed H9c2 cell viability and KCNH2 level and triggered
apoptosis, ROS production and mitochondrial depolarization, while Sigmar1 upregulation
reversed its effects. Moreover, KCNH2 silencing neutralized the effect of
Sigmar1 up-regulation on H9c2 cell viability, apoptosis, and ROS production.

Conclusion:
Amiodarone facilitates the apoptosis of H9c2 cells by restraining Sigmar1
expression and blocking KCNH2-related potassium channels.
Cardiac Toxicity in the Treatment of Light Chain Amyloidosis: Systematic
Review of Clinical Studies
Authors: Jattin-Balcázar Jairo Javier , Quiroga-Ramírez Paula Andrea 
Published: January 2024

Background:
Light chain amyloidosis (AL) is a progressive and a fatal disease that
primarily affects cardiac tissue. Although the current approach to anti-amyloidosis treatments
has managed to reduce amyloidosis morbimortality, the dynamics of cardiac adverse events are
unknown.

Objective:
to provide evidence about reported cardiac toxicity during treatment of AL amyloidosis through a systematic review of the literature.

Methods:
A search was performed for registered clinical trials on ClinicalTrials.gov filtered for
AL amyloidosis up to December 31, 2022. Studies were filtered by those that reported intervention in patients with AL amyloidosis and that had reported adverse events. The type of study, the
intervention performed, and the frequency of reported cardiac adverse events were discriminated
from each trial.

Results:
25 clinical trials were analyzed, representing a population of 1,542 patients, among
whom 576 (38.95%) adverse events were reported, 326 being serious (SAE) and 242 nonserious (nSAE). The most frequent SAEs were cardiac failure, atrial fibrillation, and cardiac arrest, while the most frequent nSAEs were palpitations, atrial fibrillation, and sinus tachycardia.

Conclusion:
cardiac toxicity during treatment for amyloidosis seems common, and it is important to evaluate the relationship of therapies with its occurrence.
Fucoxanthin Enhances the Antifibrotic Potential of Placenta-derived Mesenchymal Stem Cells in a CCl4-induced Mouse Model of Liver
Authors: Slautin Vasilii , Konyshev Konstantin , Gavrilov Ilya , Beresneva Olga , Maklakova Irina , Grebnev Dmitry 
Published: January 2024

Background:
The effectiveness of fucoxanthin (Fx) in liver diseases has been reported
due to its anti-inflammatory and antifibrotic effects. Mesenchymal stem cells (MSCs)-based therapy
has also been proposed as a promising strategy for liver fibrosis treatment. Recent studies have
shown that the co-administration of MSCs and drugs demonstrates a pronounced effect on liver fibrosis.

Aim:
This study aimed to determine the therapeutic potential of placenta-derived MSCs (PD-MSCs)
in combination with Fx to treat liver fibrosis and evaluate their impact on the main links of
liver fibrosis pathogenesis.

Methods:
After PD-MSCs isolation and identification, outbred ICR/CD1 mice were divided into
five groups: Control group, CCl4 group (CCl4), Fx group (CCl4+Fx), PD-MSCs group (CCl4+MSCs)
and cotreatment group (CCl4+MSCs+Fx). Biochemical histopathological investigations were
performed. Semiquantitative analysis of the alpha-smooth muscle actin (α-SMA+), matrix metalloproteinases
(MMP-9+, MMP-13+), tissue inhibitor of matrix metalloproteinases-1 (TIMP-1+) areas,
and the number of positive cells in them were studied by immunohistochemical staining.
Transforming growth factor-beta (TGF-β), hepatic growth factor (HGF), procollagen-1 (COL1α1)
in liver homogenate and proinflammatory cytokines in blood serum were determined using an enzyme
immunoassay.

Results:
Compared to the single treatment with PD-MSCs or Fx, their combined administration
significantly reduced liver enzyme activity, the severity of liver fibrosis, the proinflammatory cytokine
levels, TGF-β level, α-SMA+, TIMP-1+ areas and the number of positive cells in them,
and increased HGF level, MMP-13+, and MMP-9+ areas.

Conclusion:
Fx enhanced the therapeutic potential of PD-MSCs in CCl4-induced liver fibrosis,
but more investigations are necessary to understand the mutual impact of PD-MSCs and Fx.
Design, Synthesis and Anti-cancer Evaluation of Nitrogen-containing Derivatives of
30-Carboxyl of Gambogic Acid
Authors: Li Hong , Lin Huiping , Li Jiajun , Chen Kaixin , Chen Zanhong , Zhang Jianye , Huang Yan , Zhao Xin , Ti Huihui , Tao Yiwen 
Published: January 2024

Background:
Gambogic acid (GA) is a natural product from the resin of the Garcinia species, which
showed significant activity in the induction of apoptosis. It can be one promising lead compound for the design
and synthesis of new anticancer drugs.

Objective:
The objective of the current study is to design novel nitrogen-contained GA derivatives with better
anti-cancer activities and study the effect of the introduction of different nitrogen-contained groups on the activity
of GA.

Methods:
The designed 15 derivatives were synthesized via esterification or amidation of 30-carboxylate. The
synthetic compounds were characterized via different spectroscopic techniques, including X-ray single crystal
diffraction, MS and NMR. The cytotoxic activity of the designed derivatives was evaluated in vitro against A549,
HepG-2, and MCF-7 cell lines using methyl thiazolyl tetrazolium (MTT) test.

Results:
15 nitrogen-contained GA derivatives were successfully synthesized and established. Based on the IC50
values, compounds 9, 10, 11 and 13 showed stronger inhibitory effects on A549, HepG-2, MCF-7 cell lines than
GA, while 9 is the most active compound with IC50 value of 0.64-1.49 μM. Most derivatives of GA with esterification
of C-30 including cyano-benzene ring were generally weaker than those of pyrimidinyl-substituted derivatives.
In addition, length of alkyl linkers between C-30 of GA and nitrogen-contained group produced different
effects on A549, HepG-2 and MCF-7 cell lines.

Conclusion:
The structure-activity relationship results show that aromatic substituent and linker length play important
roles to improve the anticancer activities, while compound 9 with pyrimidine substituent and C-C-C linkers
is the most active derivative against tested cell lines, and is a promising anti-cancer agent for further development.
Análisis sobre la interpretación y aplicación jurídica de la responsabilidad social de las empresas privadas, regulada por el artículo 25 de la Constitución Política de los Estados Unidos Mexicanos
Authors: Santiago Hernández Ana Rosa 
Published: January 2024

En este artículo se analiza la interpretación jurídica de la responsabilidad social de las empresas privadas, misma que se encuentra contenida en el artículo 25 constitucional. En principio, se intenta definir cuáles son las implicaciones de esa responsabilidad social y ante quiénes deben ser socialmente responsables dichas empresas. Posteriormente se hace un análisis de la interpretación constitucional por tratarse de un dispositivo de ese rango. Se observó que en la realidad no se tiene bien definida la responsabilidad social, dando lugar a diversas interpretaciones y a una aplicación voluntaria y desvirtuada por parte de las empresas, que sólo la aplican por cuestiones de imagen o para desgravar y no en beneficio de la sociedad. Concluyendo con la necesidad de una ley reglamentaria que regule la responsabilidad social que permita su seguimiento, control y sanciones.
Current Issues of International Legal Regulation of the Convergence of Transport and Logistics Systems within the EAEU
Authors: Drozdova M. A. 
Published: January 2024

With the development of integration processes on the territory of the Eurasian Economic Union, the convergence of transport and logistics systems is a necessary condition for both transport connectivity and the development of trade relations. The current geopolitical situation, which led to an increase in traffic and within the EEU and the redirection of transport flows to the East, requires a change in the paradigm of the legal regulation of the transport industry at the interstate level.Aim . Reveal the main problems of international legal regulation of the convergence of transport and logistics ecosystems in the Eurasian region.Tasks. It is proposed to consider the main trends of the formation of an array of legal regulation of the transport and logistics industry in the EAEU as part of the implementation of a common transport policy and the implementation of major infrastructure projects, such as the Eurasian transport framework, to analyze the relevance of the main EAEU documents in the field of transport and logistics, to present the basic elements of the new paradigm of the EAEU unified transport system, including railway transport.Methods. When conducting the research, comparative-legal, formal-legal methods, synthesis, deduction, induction, as well as abstraction and the systematic method were used.Results . The necessity of a significant change in the approach to the formation of an effective model of international legal regulation of transport integration within the EAEU, clarification of the main directions of a common transport policy of the member states of the association, ensuring its competitiveness at the world level, transport and technological sovereignty of Russia and other partner countries, full realization of the transit potential of the union, as well as the effective functioning of the industry in the conditions of Industry 4.0 is substantiated.Conclusions . The proposed integrated approach will make it possible to fully implement the adopted Strategic Directions for the development of Eurasian economic integration until 2025, to lay the foundations for a new stage of integration of transport and logistics systems within the EAEU with the achievement of the set significant milestones in the interests of the countries and peoples of our integration association.
An Accounting Classification System Using Constituency Analysis and Semantic Web Technologies
Authors: Li Chang-Wei , Chou Chi-Chun , Yen Ju-Chun 
Published: January 2024

ABSTRACT
To aid accountants in making professional judgments and decisions regarding the accounting methods for transactions, we propose a classification system by integrating computational linguistics with semantic web technologies. We use constituency parsing to convert the classification rules in accounting standards into a machine-processable data structure: Resource Description Framework (RDF) triples. When an accounting classification question is input, the system converts it into an RDF triple, compares it with the established triples of different accounting methods, and subsequently identifies the most appropriate accounting method. We showcased and evaluated our proposed model using IFRS 9 and IAS 28. Our study provides both scholarly and practical applications by (1) incorporating computational linguistics and semantic web technologies to create an interpretable, process-traceable, and explainable classification system aligned with regulatory requirements; and (2) proving that the knowledge-based model can be established without substantial training data, enhancing its accessibility and utility for accounting professionals.
Data Availability: Data are available from the authors upon request.