bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fundamental cognizance and scholarly exploration of immune-related notions in head and neck squamous cell carcinoma (SCC) from 2012 to 2022: a bibliometric analysis
Authors: Miao Rui , Ma Wenyang , Liu Shujie 
Published: October 2023

Abstract
Background and Objective
Immunization holds profound research significance and promising application potential in the realm of head and neck squamous cell carcinoma (HNSCC). Regrettably, only a paucity of bibliometric data has been dedicated to a systematic examination of this domain. The present study endeavors to holistically assess the research landscape encompassing immunization and allied concepts in HNSCC. It shall encompass an exhaustive review of countries, institutions, authors, and journals involved in this sphere, while also evaluating the knowledge framework through keyword co-occurrence analysis.
Methods
The method employed for subject retrieval involved accessing the Web of Science core collection to obtain works and reviews concerning HNSCC immunity. Subsequently, bibliometric analysis was conducted utilizing the CiteSpace and VOSviewer software.
Results
Between 1 January 2012 and 31 October 2022, a total of 1,744 English works and reviews were considered in the analysis. The research contributions emanated from 402 institutions, primarily from 57 countries and regions. In terms of journals, CANCERS published the highest number of works, other journals in the fields of molecular biology, biological immunology, and clinical medicine also made substantial contributions.Over the course of time, the landscape of research hotspots gracefully evolved. Certain illustrious terms such as “open label”, “t cell”, “immunotherapy”, and “tumor microenvironment” elegantly emerged, gracing the scholarly stage with their profound frequency.
Conclusion
This study presents a thorough and all-encompassing assessment of HNSCC research within the context of immunity, achieved through the utilization of bibliometric and visualization methodologies. By offering insights into the dynamic evolution of immune-related concepts in HNSCC, this paper will undoubtedly aid researchers in gaining a deeper understanding of this complex domain.
Exosomes from adipose-derived stem cells enhance functional recovery after spinal cord injury by inhibiting ferroptosis and promoting the survival and function of endothelial cells through the Nrf2/SLC7A11/GPX4 pathway
Authors: Wu Shengting , Chen Zhiheng , Wu Yinhao , Shi Qiang , Yang Erzhu , Qian Yuxuan , Zhang Baokun , Lian Xiaofeng , Xu Jianguang 
Published: October 2023

Abstract
Background
Spinal cord injury (SCI) is a devastating disease that causes major motor, sensory and autonomic dysfunctions. Currently, there is a lack of effective treatment methods. In this study, we aimed to investigate the potential mechanisms of Exosomes from adipose-derived stem cells (ADSC-Exos) in reducing ferroptosis and promoting angiogenesis after spinal cord injury.
Methods
We isolated ADSC-Exos, the characteristics of which were confirmed. In vitro, we tested the potential of ADSC-Exos to promote the survival and function of human brain microvascular endothelial cells (HBMECs) with tube formation assays, scratch assays and Transwell assays and analyzed the ferroptosis of HBMECs with western blotting, immunofluorescence and biochemical detection. In vivo, we established a rat model of SCI by the modified Allen's method and locally injected ADSC-Exos to verify their efficacy.
Results
ADSC-Exos can reduce reactive oxygen species (ROS) accumulation and cell damage induced by an excessive inflammatory response in HMBECs. ADSC-Exos inhibit ferroptosis induced by excessive inflammation and upregulate the expression of glutathione peroxidase 4(GPX4) in HMBECs. It can also effectively promote proliferation, migration, and vessel-like structure formation. In vitro ADSC-Exos improved behavioral function at days 3, 7, and 14 after SCI and increased the number and density of blood vessels around the damaged spinal cord by approximately 30.4% ± 4.4%. Moreover, we found that ADSC-Exos could increase nuclear factor erythroid-2-related factor 2(Nrf2) expression and nuclear translocation, thereby affecting the expression of solute carrier family 7 member 11(SLC7A11) and GPX4, and the Nrf2 inhibitor ML385 could reverse the above changes.
Conclusion
In summary, our results suggest that ADSC-Exos may inhibit ferroptosis and promote the recovery of vascular and neural functions after SCI through the Nrf2/SLC7A11/GPX4 pathway. This may be a potential therapeutic mechanism for spinal cord injury.
A singleton pregnancy with placental chorioangioma and hydrops fetalis complicated with mirror syndrome and ritodrine-induced side effects: A case report
Authors: Wu Pei-Tzu , Huang Kun-Long , Tsai Ching-Chang , Cheng Hsin-Hsin , Lai Yun-Ju , Hsu Te-Yao 
Published: October 2023

Abstract
Background: Ritodrine hydrochloride is a widely used beta-adrenergic agonist used to stop preterm labor in Taiwan. Many side effects causing maternal morbidity and mortality have been reported. We report a case complicated with ritodrine-induced side effects and mirror syndrome that was associated with placental chorioangioma.Case presentation: A 36-year-old singleton pregnant woman at 25 6/7 weeks of gestation was administered continuous intravenous ritodrine for preterm uterine contractions. After one week of tocolytics, she presented with elevated serum levels of creatinine kinase (CK), acute kidney injury, impaired liver function and pulmonary edema. Ultrasound showed hydrops fetalis, polyhydramnios and placental thickening. Because of progressive dyspnea and rhabdomyolysis, emergency cesarean section was performed at 27 1/7 weeks of gestation. A 1000-g female infant with general subcutaneous edema was delivered and had an Apgar score of 1 at 1 min and 3 at 5 min. In addition, an 8.5-cm placental chorioangioma was found during the operation. After cesarean section and the cessation of intravenous ritodrine, her symptoms and laboratory indicators improved gradually during the postpartum period. It is difficult to identify mirror syndrome and ritodrine-induced pulmonary edema. Thus, this case is educational and worthy of discussion.Conclusion: Our case demonstrates mirror syndrome induced by chorioangioma, which is rare, and ritodrine-induced side effects. The cessation of intravenous ritodrine and delivery are the best methods to treat maternal critical status due to fluid overload.
Correlation between the times of IUI and pregnancy outcome: a systematic review and meta-analysis with trial sequential analysis of cohort study
Authors: Yu Wenjing , Wei Aiwu , Yuan Hao , Sun Zhiyu 
Published: October 2023

Abstract
Purpose
the study aims to evaluate whether increasing the times of IUI per cycle has better pregnancy outcomes for infertile couples.
Methods
PubMed, Embase, Web of science, Cochrane Library, CNKI, WanFang Data, VIP (VIP Chinese Journal Service Platform), and SinoMed (China Biomedical Literature Service), were systematically retrieved cohort studies about the times of IUI per cycle and pregnancy outcomes published before December 31, 2021. Included studies were assessed by Newcastle-Ottawa Scale (NOS) with two reviewers. The data was processed with RevMan 5.4.1 and the result of the meta-analysis were further examined by TSA 0.9.5.10 Beta version.
Results
23 cohort studies with 54208 IUI cycles were included in this study. Meta-analysis of the data reported in these 23 studies showed that the cycle pregnancy rates were higher in the double insemination group (double IUI) than in the single insemination group (single IUI) [RR = 1.14, 95% CI (1.02, 1.27), I2 = 64%, P = 0.02]. Sensitivity analysis showed that the results were stable and reliable. The bias analysis showed that there was a low possibility of publication bias in this study. TSA of 23 trials illustrate that the cumulative z-curve crossed the traditional boundary, the trial sequential monitoring boundary and the required information size(RIS).
Conclusion
compared with single IUI, double IUI per cycle can increase the cycle pregnancy rates to a small extent without increasing the incidence of biochemical pregnancy, miscarriage, or ectopic pregnancy. The results should be treated with caution and other factors should be taken into consideration when making clinical decisions.
Study Protocol: A pragmatic, cluster-randomized controlled trial to evaluate the effect of implementation of the Truenat platform/MTB assays at primary health care clinics in Mozambique and Tanzania (TB-CAPT CORE)
Authors: Leukes Vinzeigh Nicholas , Riess Friedrich , Erkosar Berra , Ntinginya Nyanda Elias , Sabi Issa , Mangu Chacha , Siyame Elimina , Mtafya Bariki , Lwilla Anange , Khosa Celso , Viegas Sofia , Madeira Carla , Machiana Antonio , Ribeiro Jorge , Garcia-Basteiro Alberto , Nguenha Dinis , Cossa Marta , Elisio Delio , Hella Jerry , Sasamalo Mohamed , Mhalu Grace , Denkinger Claudia Maria , Castro Maria del Mar , Schumacher Samuel , Tagliani Elisa , Malhotra Akash , Dowdy David , Bashir Saima , Schact Claudia , Buech Julia , Ruhwald Morten , Penn-Nicholson Adam , Kranzer Katharina 
Published: October 2023

Abstract
Background
In 2020, the WHO-approved Molbio Truenat platform and MTB assays to detect Mycobacterium tuberculosis complex (MTB) and resistance to rifampicin directly on sputum specimens. This primary health care center-based trial in Mozambique and Tanzania investigates the effect of Truenat platform/MTB assays (intervention arm) combined with rapid communication of results compared to standard of care on TB diagnosis and treatment initiation for microbiologically confirmed TB at 7 days from enrolment.
Methods
The Tuberculosis Close the Gap, Increase Access, and Provide Adequate Therapy (TB-CAPT) CORE trial employs a pragmatic cluster randomized controlled design toevaluate the impact of a streamlined strategy for delivery of Truenat platform/MTB assays testingat primary health centers. Twenty-nine centers equipped with TB microscopy units were selected to participate in the trial. Among them, fifteen health centers were randomized to the intervention arm (which involves onsite molecular testing using Truenat platform/MTB assays, process process optimization to enable same-day TB diagnosis and treatment initiation, and feedback on Molbio platform performance) or the control arm (which follows routine care, including on-site sputum smear microscopy and the referral of sputum samples to off-site Xpert testing sites). The primary outcome of the study is the absolute number and proportion of participants with TB microbiological confirmation starting TB treatment within 7 days of their first visit. Secondary outcomes include time to bacteriological confirmation, health outcomes up to 60 days from first visit, as well as user preferences, direct ccost,and productivity analyses.
Ethics and dissemination
TB-CAPT CORE trial has been approved by regulatory and ethical committees in Mozambique and Tanzania, as well as by each partner organization. Consent is informed and voluntary, and confidentiality of participants is maintained throughout. Study findings will be presented at scientific conferences and published in peer-reviewed international journals.
Trial Registration
US National Institutes of Health’s ClinicalTrials.gov, NCT04568954. Registered 23 September 2020.
Proportions and risk factors of chronic obstructive pulmonary disease and preserved ratio impaired spirometry, and association with small airway disease, in the screening positive older population from china: a cross-sectional study
Authors: Sang Le , Gong Xia , Huang Yunlei , Sun Jian 
Published: October 2023

Abstract
Background Early diagnosing Chronic Obstructive Pulmonary Disease (COPD) is relatively difficult. Therefore, the concepts of preserved ratio impaired spirometry (PRISm) and small airway disease (SAD) were proposed, expecting to achieve early diagnosis for COPD. Besides, the occurrence of COPD is positively related to age. However, the relation among COPD, PRISm, and SAD still requires clarification. Thus, we estimated the proportions and risk factors of COPD and PRISm in the screening positive participants, and searched the methods of early diagnosing COPD through the indicators of SAD. Methods A total of 53641 residents aged more than 60 years old from Shaoxing City, Zhejiang Province, China, completed a series of screening projects. And 2327 of screening positive participants ultimately finished bronchodilator tests. The data were statistically analyzed to figure out the proportions and risk factors of COPD and PRISm, and the efficacy of early diagnosing COPD by the indicators of SAD. Results Totally 2229 screening positive participants were included, the proportion of PRISm was 6.3% (141/2229), and of COPD was 78.2% (1743/2229). The results of statistical analysis showed that COPD patients were more likely to be smokers, males, older, and with higher questionnaire scores. Additionally, COPD patients had lower maximal mid-expiratory flow (MMEF), forced expiratory flow (FEF)75pred, and FEF50pred. And it was found that male sex and the presence of respiratory symptoms might lead to COPD and PRISm. Also, the methods of early diagnosing COPD by the indicators of SAD might be acceptable. Conclusion There is a close relation between COPD and decreased small airway function (SAF) among the participants included. Age, smoking, male sex, worse SAF, and respiratory symptoms might cause the progressing from normal people to PRISm, then to COPD patients. Besides, early diagnosing COPD by the indicators of SAD might be feasible. In the future, early diagnosis for COPD requires further research.
The relationship between physical activity and thyroid function in American adults: A survey from the NHANES database
Authors: Tian Lijun , Lu Cihang , Teng Weiping 
Published: October 2023

Abstract

Objective
Physical activity (PA) is closely related to our lives, and the effects of PA on thyroid function have not been elucidated.
Methods
Using data from the National Health and Nutrition Examination Survey (NHANES) 2007–2012, we included 5877 participants and analyzed the associations of thyroid function with weekly physical activity (PAM, expressed in metabolic equivalents of task) and physical activity time (PAT) in American adults. Univariate and multivariate logistic analyses were used to demonstrate the associations of PAM and PAT with the primary outcome. Linear regression analysis was performed to determine the associations between thyroid biochemical indicators/diseases and PAM/PAT.
Results
Our study revealed noticeable sex differences in daily PA among the participants. With the increase in PAM, the risk of overt hypothyroidism [p = 0.02, OR (95% CI) = 3.07 (1.24, 7.58)] and subclinical hyperthyroidism [p = 0.03, OR (95% CI) = 3.25 (1.12, 9.45)] increased in men. The risk of subclinical hyperthyroidism [p < 0.001, OR (95% CI) = 5.89 (1.85, 18.80)], overt hypothyroidism [p < 0.001, OR (95% CI) = 8.70 (2.80, 27.07)] and autoimmune thyroiditis (AIT) [p = 0.03, OR (95% CI) = 1.42 (1.03, 1.97)] increased with PAT in adult men. TSH, FT4, TT4, FT3, and TT3 levels also varied to a certain extent with changes in PAM and PAT.
Conclusion
The amount of daily physical activity of American adults has a certain influence on changes in thyroid function, including thyroid hormone levels and thyroid diseases. Higher PAM and PAT were strongly associated with the risk of clinical hypothyroidism, hyperthyroidism and AIT in men.
Computed tomography-guided core needle biopsy for pulmonary nodules: comparison between with and without co-axial technique
Authors: Zhu Qunqun , Zhang Li , Xia Fengfei , Shi Yi-Bing , Zhu Lei , Liang Xianxian 
Published: October 2023

Abstract

Background
Computed tomography (CT)-guided core needle biopsy (CNB) procedures are widely used to evaluate and assign a diagnosis to pulmonary nodules (PNs), with the co-axial technique (CAT) being widely used to aid this procedure. The present study was designed to compare the safety and diagnostic performance of using CT-guided CNB procedures to diagnose PNs when performing these procedures with and without CAT.
Methods
Between January 2017 and December 2019, this retrospective analysis incorporated consecutive patients undergoing CT-guided CNB for PNs, after which data of interest were compared between these groups.
Results
In total, CT-guided CNB procedures were performed with and without CAT in 111 and 108 respective patients. The CAT group exhibited a significantly lower mean needle pathway number as compared to the non-CAT group (P < 0.001), whereas the mean sample number in the CAT group was significantly greater than that in the non-CAT group (P < 0.001). The mean CNB procedural duration in the CAT group was also significantly reduced as compared to the non-CAT group (P < 0.001). While both groups exhibited similar diagnostic accuracy (98.3% vs. 96.3%, P = 0.600), the CAT group outperformed the non-CAT group with respect to diagnostic yield (81.4% vs. 68.5%, P = 0.026). Pneumothorax and pulmonary hemorrhage rats were similar in these groups (P = 0.092 and 0.164).
Conclusion
Relative to CT-guided CNB procedures performed without the CAT, those performed with the CAT exhibited fewer needle pathways and shorter operative duration, while increasing obtained samples and improving diagnostic yields.
Tumoricidal properties of Thymoquinone on human adenocarcinoma cells via the modulation of autophagy
Authors: Moulana Mohammad Saleh , Haiaty Sanya , Bazmani Ahad , Shabkhizan Roya , Moslehian Marziyeh Sadat , Sadeghsoltani Fatemeh , Mostafazadeh Mostafa , Asadi Mohammad Reza , Talebi Mehdi , Jafari Zahra , Morovati Mohammad Reza , Farzaei Mohammad Hosein , Rahbarghazi Reza 
Published: October 2023

Abstract
Colorectal cancer (CRC) is deadly anaplastic changes in the gastrointestinal tract with high-rate mortality. In recent years, the application of phytocompounds has been extended along with different therapeutic protocols. Here, we monitored the effects of Thymoquinone (TQ) on autophagy via mitochondrial function after modulation of the Wnt/β-catenin signaling pathway.
Human colorectal adenocarcinoma HT-29 cells were treated with TQ (60 μM) and Wnt3a inhibitor (LGK974; 15 μM) for 48 hours. The survival rate was evaluated using an MTT assay. The expression of Wnt-related factors (c-Myc, and Axin), angiogenesis (VE-Cadherin), and mitophagy-related factors (PINK1, OPTN) was assessed using real-time PCR assay. Protein levels of autophagy factors (Beclin-1, LC3, and P62) were monitored using western blotting. Using flow cytometry analysis, the intracellular accumulation of Rhodamine 123 was evaluated. The migration properties were analyzed using a scratch wound healing assay.
Data indicated that TQ can reduce the viability of HT-29 cells compared to the control cells (p<0.05). The expression of VE-Cadherin was inhibited while the expression of PINK1 was induced in treated cells (p<0.05). Both LGK974 and TQ-treated cells exhibited activation of autophagy flux (Beclin-1↑, LC3II/I↑, and p62↓) compared to the control group (p<0.05). TQ can increase intracellular accumulation of Rhodamine 123, indicating the inhibition of efflux mechanisms in cancer cells. Along with these changes, the migration of cells was also reduced.
TQ is potential phytocompounds to alter the dynamic growth of human colorectal HT-29 cells via the modulation of autophagy, and mitophagy-related mechanisms.
Analyzing Fuzzy Boundary Value Problems: A Study on the Influence of Mitochondria and ER Fluxes on Calcium Ions in Neuron Cells
Authors: Bhattacharyya Rituparna , Jha Brajesh Kumar 
Published: October 2023

Abstract
Cytosolic-free calcium ions play an important role in various physical and physiological processes. A vital component of neural signaling is the free calcium ion concentration often known as the second messenger. There are many parameters that effect the cytosolic free calcium concentration like buffer, voltage-gated ion channels, Endoplasmic reticulum, Mitochondria, etc. The endoplasmic reticulum (ER) serves as a repository of free calcium ions and contains a significant quantity of calcium. Mitochondria are small organelles located within the nervous system that are involved in processes within cells such as calcium homeostasis management, energy generation, response to stress, and cell demise pathways. Till date, the researchers studied such physiological processes using a deterministic way. In this work, we, have analyzed such problems considering the uncertainty of different parameters using a Fuzzy environment. A mathematical model with fuzzy boundary values has been developed to study the effect of Mitochondria and ER fluxes on free Calcium ions. The intended findings are displayed utilizing the physiological understanding that amyloid beta plaques and tangles of neurofibrillary fibers have been identified as the two main causes of AD. The key conclusion of the work is the investigation of Ca 2+ for healthy and cells affected by Alzheimer's disease, which may aid in study of such processes for computational scientists and medical practitioners. In this paper, we used a strategy that relies on the characteristics of linear transformation. Additionally demonstrated that if a similar precise problem has a novel solution, it also solves the fuzziness of the problem. Furthermore, the comparison between the analytical approach and the generalized hukuhara derivative approach is shown here, which illustrates the benefits of the analytical approach. The simulation is carried out in MATLAB.
PPIAT: Targeted Mass Spectrometry-Based Analysis Tool for Protein-Protein Interactions
Authors: Park Jongham , Son Ahrum , Kim Hyunsoo 
Published: October 2023

Abstract
Motivation: Protein networking is critical to understanding the biological functions of proteins and the underlying mechanisms of disease. However, identifying physical protein-protein interactions (PPIs) can be challenging. To gain insights into target proteins that interact with a particular disease, we need to profile all the proteins involved in the disease beforehand. Although the cross-linking mass spectrometry (XL-MS) method is a representative approach to identify physical interactions between proteins, calculating theoretical mass values for application to targeted mass spectrometry can be difficult.
Results: To address this challenge, our research team developed PPIAT, a web application that integrates information on reviewed human proteins, protein-protein interactions, cross-linkers, enzymes, and modifications. PPIAT enables users to identify physical protein-protein interactions using targeted mass spectrometry. All functions of PPIAT are available for free on the web application, making it a valuable tool for researchers studying protein-protein interactions.
Availability and Implementation: PPIAT is freely available at ppiat.cnu.ac.kr/.
Contact: kimlab@cnu.ac.kr