bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visualization of root extracellular traps in an ectomycorrhizal woody plant (Pinus densiflora) and their interactions with root-associated bacteria
Authors: Shirakawa Makoto , Matsushita Norihisa , Fukuda Kenji 
Published: November 2023

Abstract
Main conclusion
Extracellular traps in the primary root of Pinus densiflora contribute to root-associated bacterial colonization. Trapped rhizobacteria induce the production of reactive oxygen species in root-associated, cap-derived cells.

Abstract
Ectomycorrhizal (ECM) woody plants, such as members of Pinaceae and Fagaceae, can acquire resistance to biotic and abiotic stresses through the formation of mycorrhiza with ECM fungi. However, germinated tree seedlings do not have mycorrhizae and it takes several weeks for ectomycorrhizae to form on their root tips. Therefore, to confer protection during the early growth stage, bare primary roots require defense mechanisms other than mycorrhization. Here, we attempted to visualize root extracellular traps (RETs), an innate root defense mechanism, in the primary root of Pinus densiflora and investigate the interactions with root-associated bacteria isolated from ECM and fine non-mycorrhizal roots. Histological and histochemical imaging and colony-forming unit assays demonstrated that RETs in P. densiflora, mainly consisting of root-associated, cap-derived cells (AC-DCs) and large amounts of root mucilage, promote bacterial colonization in the rhizosphere, despite also having bactericidal activity via extracellular DNA. Four rhizobacterial strains retarded the mycelial growth of a pathogenic strain belonging to the Fusarium oxysporum species complex in dual culture assay. They also induced the production of reactive oxygen species (ROS) from host tree AC-DCs without being excluded from the rhizosphere of P. densiflora. Applying three Paraburkholderia strains, especially PM O-EM8 and PF T-NM22, showed significant differences in the ROS levels from the control group. These results reveal the indirect contributions of rhizobacteria to host root defense and suggest that root-associated bacteria could be a component of RETs as a first line of defense against root pathogens in the early growth stage of ECM woody plants.
Oral health status and oral habits of children and adolescents with hemophilia: a report from the children’s hemophilia comprehensive care center of China
Authors: Li Yue , Liu Guoqing , Wu Runhui , Yu Guoxia 
Published: November 2023

AbstractIn recent years, the diagnosis and treatment of hemophilic children in China has significantly improved. However, oral health conditions, which affect quality of life, haven’t received attention in this population. To explore the oral health status and oral hygiene of children and adolescents with hemophilia in the Children’s Hemophilia Comprehensive Care Center of China. Dental and oral hygiene examinations were performed in children and adolescents with hemophilia who visited Beijing Children’s Hospital. DMFT/dmft (decayed, missing, filled teeth in permanent and primary teeth) was assessed according to World Health Organization (WHO) criteria. The simplified oral hygiene index (OHI-S) was used to evaluate the oral hygiene condition of the subjects. Questionnaires were completed by their parents. SPSS 21.0 was used for statistical analysis. A total of 114 children and adolescents were enrolled. The caries prevalence was 57.4%, 72.2% and 41.2% in primary, mixed and permanent dentitions respectively. The filling rates were 14.4%, 13.9%, and 11.4%, respectively, and the OHI-S scores of the three dentition groups were 1.49 ± 0.46, 1.57 ± 0.43, and 1.76 ± 0.46, respectively. A total of 103 valid questionnaires were collected. Sixty-nine children (67%) didn’t brushed their teeth 2 times a day. Nearly half of the parents knew little about fluoride toothpaste. Multiple linear regression analysis revealed that brushing teeth with the help of parents had a significant positive impact on OHI-S. Conclusion: Dental health was unsatisfactory among hemophilic children and adolescents. The caries filling rates were low. Patients and their parents did not give much attention to oral health.

What is Known:• Caries and gingivitis are the two main oral diseases that affect children with hemophilia.• However, the oral health conditions of children and adolescents with hemophilia have not received much attention in China.

What is New:• This is the first study concentrating on the dental health of children with hemophilia in China.• Dental health was unsatisfactory among children and adolescents with hemophilia in China.
Optimal transport methods for combinatorial optimization over two random point sets
Authors: Goldman Michael , Trevisan Dario 
Published: November 2023

AbstractWe investigate the minimum cost of a wide class of combinatorial optimization problems over random bipartite geometric graphs in $$\mathbb {R}^d$$

R

d

where the edge cost between two points is given by a pth power of their Euclidean distance. This includes e.g. the travelling salesperson problem and the bounded degree minimum spanning tree. We establish in particular almost sure convergence, as n grows, of a suitable renormalization of the random minimum cost, if the points are uniformly distributed and $$d \ge 3, 1\le p<d$$

d
≥
3
,
1
≤
p
<
d

. Previous results were limited to the range $$p<d/2$$

p
<
d
/
2

. Our proofs are based on subadditivity methods and build upon new bounds for random instances of the Euclidean bipartite matching problem, obtained through its optimal transport relaxation and functional analytic techniques.
Computing Characteristic Polynomials of Hyperplane Arrangements with Symmetries
Authors: Brysiewicz Taylor , Eble Holger , Kühne Lukas 
Published: November 2023

AbstractWe introduce a new algorithm computing the characteristic polynomials of hyperplane arrangements which exploits their underlying symmetry groups. Our algorithm counts the chambers of an arrangement as a byproduct of computing its characteristic polynomial. We showcase our implementation, based on , on examples coming from hyperplane arrangements with applications to physics and computer science.
Longitudinal Analysis of Dysphagia and Factors Related to Postoperative Pneumonia in Patients Undergoing Esophagectomy for Esophageal Cancer
Authors: Kaneoka Asako , Inokuchi Haruhi , Ueha Rumi , Sato Taku , Goto Takao , Yamauchi Akihito , Seto Yasuyuki , Haga Nobuhiko 
Published: November 2023

AbstractFew studies have quantified longitudinal changes in swallowing in patients undergoing esophagectomy for esophageal cancer. This study longitudinally analyzed the changes in the Modified Barium Swallow Study Impairment Profile (MBSImP™) scores, swallowing kinematic measurements, and swallowing-related symptoms in patients undergoing esophagectomy. We also examined the association between identified swallowing impairment and aspiration pneumonia after surgery. We included consecutive patients who underwent esophagectomy and completed laryngoscopy and videofluoroscopy before, two weeks, and three months after surgery. We analyzed physiological impairments using the MBSImP. We also assessed the swallowing kinematics on a 5 mL thickened liquid bolus at three time points. Vocal fold mobility was assessed using a laryngoscope. Repeated measures were statistically examined for longitudinal changes in swallowing function. The association between the significant changes identified after esophagectomy and aspiration pneumonia was tested. Twenty-nine patients were included in this study. Preoperative swallowing function was intact in all participants. The timing of swallowing initiation and opening of the pharyngoesophageal segment remained unchanged after surgery. Tongue base retraction and pharyngeal constriction ratio worsened two weeks after surgery but returned to baseline levels three months after surgery. Three months after surgery, hyoid displacement and vocal fold immobility did not fully recover. Aspiration pneumonia occurred in nine patients after surgery and was associated with postoperative MBSImP pharyngeal residue scores. Decreased hyoid displacement and vocal fold immobility were observed postoperatively and persisted for a long time. The postoperative pharyngeal residue was associated with pneumonia and thus should be appropriately managed after surgery.
Fluid assessment, fluid balance, and fluid overload in sick children: a report from the Pediatric Acute Disease Quality Initiative (ADQI) conference
Authors: Selewski David T. , Barhight Matthew F. , Bjornstad Erica C. , Ricci Zaccaria , de Sousa Tavares Marcelo , Akcan-Arikan Ayse , Goldstein Stuart L. , Basu Rajit , Bagshaw Sean M. ,   on behalf of the Pediatric the Acute Disease Quality Initiative (ADQI) Consensus Committee Members, Alobaidi Rashid , Askenazi David J. , Barreto Erin , Bayrakci Benan , Bignall O. N. Ray , Brophy Patrick , Charlton Jennifer , Chanchlani Rahul , Conroy Andrea L. , Deep Akash , Devarajan Prasad , Dolan Kristin , Fuhrman Dana , Gist Katja M. , Gorga Stephen M. , Greenberg Jason H. , Hasson Denise , Heydari Emma , Iyengar Arpana , Jetton Jennifer , Krawczeski Catherine , Meigs Leslie , Menon Shina , Morgan Catherine , Morgan Jolyn , Mottes Theresa , Neumayr Tara , Soranno Danielle , Stanski Natalja , Starr Michelle , Sutherland Scott M. , Symons Jordan , Vega Molly , Zappitelli Michael , Ronco Claudio , Mehta Ravindra L. , Kellum John , Ostermann Marlies 
Published: November 2023

Abstract
Background
The impact of disorders of fluid balance, including the pathologic state of fluid overload in sick children has become increasingly apparent. With this understanding, there has been a shift from application of absolute thresholds of fluid accumulation to an appreciation of the intricacies of fluid balance, including the impact of timing, trajectory, and disease pathophysiology.

Methods
The 26th Acute Disease Quality Initiative was the first to be exclusively dedicated to pediatric and neonatal acute kidney injury (pADQI). As part of the consensus panel, a multidisciplinary working group dedicated to fluid balance, fluid accumulation, and fluid overload was created. Through a search, review, and appraisal of the literature, summative consensus statements, along with identification of knowledge gaps and recommendations for clinical practice and research were developed.

Conclusions
The 26th pADQI conference proposed harmonized terminology for fluid balance and for describing a pathologic state of fluid overload for clinical practice and research. Recommendations include that the terms daily fluid balance, cumulative fluid balance, and percent cumulative fluid balance be utilized to describe the fluid status of sick children. The term fluid overload is to be preserved for describing a pathologic state of positive fluid balance associated with adverse events. Several recommendations for research were proposed including focused validation of the definition of fluid balance, fluid overload, and proposed methodologic approaches and endpoints for clinical trials.
Regionale Innovations- und Wirtschaftspolitik in Zeiten transformativen Wandels: Der CORIS-Ansatz als Orientierungsrahmen
Authors: Trippl Michaela , Benner Maximilian , Baumgartinger-Seiringer Simon 
Published: November 2023

ZusammenfassungUnsere Zeit ist geprägt von multiplen Krisen wie dem Klimawandel, Pandemien, geopolitischen Unsicherheiten sowie der Zunahme sozialer und territorialer Ungleichheiten. Diese und andere große gesellschaftliche Herausforderungen machen einen umfassenden transformativen Wandel, also maßgebliche Umstellungen unserer Produktions- und Konsumptionsmuster, erforderlich. Die geänderten Kontextbedingungen haben auch weitreichende Implikationen für die regionale Innovations- und Wirtschaftspolitik. Etablierte Ansätze wie Clusterförderung oder intelligente Spezialisierung sind vorrangig auf die Förderung von regionalem Wirtschaftswachstum und Wettbewerbsfähigkeit fokussiert und damit für die Bewältigung drängender gesellschaftlicher Herausforderungen unzureichend. Dieser Artikel plädiert dafür, regionale Innovations- und Wirtschaftspolitik neu auszurichten. Dieser kommt verstärkt die Aufgabe zu, die Initiierung und Beschleunigung territorialer Transformationsprozesse in Richtung Nachhaltigkeit aktiv zu unterstützen, gesellschaftlich wünschenswerte Innovationen zu fördern und gemeinsam mit anderen Akteuren alternative regionale Entwicklungsagenden zu gestalten. Dieser Artikel legt dar, wie der Ansatz der herausforderungsorientierten regionalen Innovationssysteme zu einer solchen Neuausrichtung der regionalen Innovations- und Wirtschaftspolitik beitragen kann und welche Implikationen sich daraus für die Praxis ergeben. Als Beispiel für eine solche Neuausrichtung stellt der Artikel Aktivitäten zur Nachnutzung stillgelegter Industriestandorte im Rahmen des Projekts „RIS4Danu“ vor.
A Novel Transcatheter Device for the Edge-to-Edge Treatment of Tricuspid Regurgitation: A Preliminary Evaluation
Authors: Salurso Eleonora , Perico Francesca , Pappalardo Fabio , Gard Marco , Antoniotti Matteo , Passanante Eugenio , Zanotti Daniele , De Bonis Michele , Alfieri Ottavio , Vismara Riccardo 
Published: November 2023

AbstractTricuspid regurgitation (TR) is the most common pathology of the tricuspid valve (TV), with significant mortality in severe cases. A well-established strategy to treat TR is represented by the clover surgical technique, which consists of stitching together the free edges of TV leaflets, producing a clover-shaped valvular orifice. Transcatheter treatments for TR constitute a valuable alternative for high-risk patients. In this work we investigated haemodynamic performances and safety of a novel device (StarTric device (STD)) aiming to perform the clover technique via percutaneous access. To assess haemodynamic performances, STD and clover were applied on porcine pathological TVs and tested. Fluid dynamic indexes of both strategies were compared to the pathological model. To evaluate device safety, forces exchanged between device and leaflets were compared to the extraction force (EF) required to STD to completely pass through the leaflet. Clover technique and STD induced a comparable TV backflow reduction (48% and 47%, respectively), with associated increase of TV flow in all tested conditions. Diastolic transvalvular pressure similarly increased indicating a reduction, though not significant, of the valvular orifice. Forces ranged from 1N to 1.71N, compared to an EF of 22.16 ± 8.6N. Force varied significantly amongst different working conditions (normotensive, mild, and severe hypertensive) for each leaflet, whilst no significative variation was found on different leaflets in the same working condition. In the adopted experimental scenario, STD demonstrated comparable efficacy to the surgical strategy in restoring TV haemodynamic. The forces acting on the leaflets following STD implantation were far lower when compared to EFs.
The ATR inhibitor berzosertib acts as a radio- and chemosensitizer in head and neck squamous cell carcinoma cell lines
Authors: Schnoell Julia , Sparr Carmen , Al-Gboore Sega , Haas Markus , Brkic Faris F. , Kadletz-Wanke Lorenz , Heiduschka Gregor , Jank Bernhard J. 
Published: November 2023

AbstractAlterations in the DNA damage response play a crucial role in radio- and chemoresistance of neoplastic cells. Activation of the Ataxia telangiectasia and Rad3-related (ATR) pathway is an important DNA damage response mechanism in head and neck squamous cell carcinoma (HNSCC). Berzosertib, a selective ATR inhibitor, shows promising radio- and chemosensitizing effects in preclinical studies and is well tolerated in clinical studies. The aim of this study was to elucidate the effect of berzosertib treatment in combination with radiation and cisplatin in HNSCC. The HNSCC cell lines Cal-27 and FaDu were treated with berzosertib alone and in combination with radiation or cisplatin. Cell viability and clonogenic survival were evaluated. The effect of combination treatment was evaluated with the SynergyFinder or combination index. Apoptosis was assessed via measurement of caspase 3/7 activation and migration was evaluated using a wound healing assay. Berzosertib treatment decreased cell viability in a dose-dependent manner and increased apoptosis. The IC50 of berzosertib treatment after 72 h was 0.25–0.29 µM. Combination with irradiation treatment led to a synergistic increase in radiosensitivity and a synergistic or additive decrease in colony formation. The combination of berzosertib and cisplatin decreased cell viability in a synergistic manner. Additionally, berzosertib inhibited migration at high doses. Berzosertib displays a cytotoxic effect in HNSCC at clinically relevant doses. Further evaluation of combination treatment with irradiation and cisplatin is strongly recommended in HNSCC patients as it may hold the potential to overcome treatment resistance, reduce treatment doses and thus mitigate adverse events.
Mosquitoes and Potatoes: How Local Climatic Conditions Impede Development
Authors: Malpede Maurizio , Falchetta Giacomo , Shayegh Soheil 
Published: November 2023

AbstractThe historical diffusion of the potato in the Old World serves as an example of the contribution of technological innovations to socio-economic growth and development (Nunn and Qian in Q J Econ 126(2):593–650, 2011). Climate-related diseases, on the other hand, might offset some of these benefits. Here we examine the long-term impact of malaria on the potato-driven growth of the population and urbanization in the Old World during the 18th and 19th centuries. We exploit local variations in environmental suitability both for potato and for malaria transmission to estimate and compare the impact of potato cultivation on population and urbanization in highly endemic to non-endemic areas at a high level of spatial disaggregation. We show that local climate conditions ideal for malaria transmission counteracted the potential benefits of introducing the potato to the Old World, which are conversely found to be strong and positive in non-endemic regions. These results highlight the interplay between technological change, public health, and development outcomes.
Logical Instrumentalism and Anti-exceptionalism about Logic
Authors: Commandeur Leon 
Published: November 2023

AbstractThis paper critically examines logical instrumentalism as it has been put forth recently in the anti-exceptionalism about logic debate. I will argue that if one wishes to uphold the claim that logic is significantly similar to science, as the anti-exceptionalists have it, then logical instrumentalism cannot be what previous authors have taken it to be. The reason for this, I will argue, is that as the position currently stands, first, it reduces to a trivial claim about the instrumental value of logical systems, and second, by its denial that logic aims to account for extra-systemic phenomena it significantly differs from science, in contrast with the AEL agenda. I will conclude by proposing a different kind of logical instrumentalism that I take to have a broad appeal, but especially for anti-exceptionalists, for it is developed as analogous to—and thus much closer aligned with—scientific instrumentalism.
Comprehensive phytochemical characterization of Persea americana Mill. fruit via UPLC/HR-ESI–MS/MS and anti-arthritic evaluation using adjuvant-induced arthritis model
Authors: Waly Dina Atef , Zeid Aisha Hussein Abou , Attia Hanan Naeim , Ahmed Kawkab A. , El-Kashoury El-Sayeda Ahmed , El Halawany Ali Mahmoud , Mohammed Reda Sayed 
Published: November 2023

AbstractPersea americana Mill. (avocado fruit) has many health benefits when added to our diet due to various pharmacological activities, such as preventing bone loss and inflammation, modulating immune response and acting as an antioxidant. In the current study, the total ethanol extract (TEE) of the fruit was investigated for in vitro antioxidant and anti-inflammatory activity via DPPH and cyclooxygenase enzyme inhibition. Biological evaluation of the antiarthritic effect of the fruit extract was further investigated in vivo using Complete Freund’s Adjuvant (CFA) arthritis model, where the average percentages of body weight change, inhibition of paw edema, basal paw diameter/weight and spleen index were estimated for all animal groups. Inflammatory mediators such as serum IL-6 and TNF-α were also determined, in addition to histopathological examination of the dissected limbs isolated from all experimental animals. Eighty-one metabolites belonging to different chemical classes were detected in the TEE of P. americana fruit via UPLC/HR-ESI–MS/MS. Two classes of lyso-glycerophospholipids; lyso-glycerophosphoethanolamines and lysoglycerophosphocholines were detected for the first time in avocado fruit in the positive mode. The TEE of fruit exhibited significant antioxidant and anti-inflammatory activity in vitro. In vivo anti-arthritic activity of the fruit TEE improved paw parameters, inflammatory mediators and spleen index. Histopathological findings showed marked improvements in the arthritic condition of the excised limbs. Therefore, avocado fruit could be proposed to be a powerful antioxidant and antiarthritic natural product.
Five-year follow-up of a posterior chamber phakic intraocular lens with a central hole for correction of myopia
Authors: Lwowski Christoph , Van Keer Karel , Ruscher Tim , Van Keer Luisa , Shajari Mehdi , Kohnen Thomas 
Published: November 2023

Abstract
Purpose
To evaluate intermediate and long-term visual outcomes and safety of a phakic intraocular posterior chamber lens with a central hole (ICL V4c) for myopic eyes.

Methods
Retrospective, consecutive case study of patients that uneventfully received a ICL V4c for myopia correction, with a 5-year postoperative follow-up. Department of Ophthalmology, Goethe University Frankfurt, Germany.

Results
From 241 eyes that underwent ICL implantation, we included 45 eyes with a mean age at surgery of 33 years ± 6 (18–48 years), with a 5 years follow-up. CDVA improved from 0.05logMAR ± 0.15 CDVA preoperatively to  − 0.00 ± 0,07 at 5 years and did not change significantly from 3 to 5 years’ time (p = 0.266). The mean spherical equivalent (SE) improved from -10.13D ± 3.39 to  − 0.45D ± 0.69. The change in endothelial cell count showed a mean decrease of 1.9% per year throughout the follow-up. Safety and efficacy index were 1.16 and 0.78, respectively. Cataract formation was seen in 2 of 241 eyes (0.8%), but in none of the 45 eyes that finished the 5-year follow-up.

Conclusions
Our data show a good intermediate and long-term stability, efficiency, and safety of ICL V4c phakic lenses in myopic eyes comparable to other known literature.
Seismological study around the Enguri dam reservoir (Georgia) based on old catalogs and ongoing monitoring
Authors: Karamzadeh Nasim , Tsereteli Nino , Gaucher Emmanuel , Tugushi Nazi , Shubladze Tamar , Varazanashvili Otar , Rietbrock Andreas 
Published: November 2023

AbstractIn the frame of the DAMAST (Dams and Seismicity) project, we deployed a dense high-fidelity seismological real-time network to investigate in detail the spatio-temporal seismicity distribution around the Enguri high dam, situated in the greater Caucasus in western Georgia. We aim at recording the weak seismicity in a 10 km distance around the dam structure. To lower the detection threshold by reducing the ambient background noise, we installed four seismic stations in shallow (ca. 20 m) and deep boreholes. From these stations, KIT1 with a depth of ca. 250 m is the deepest seismological station in Georgia. In this paper, we characterize the seismicity recorded by the local seismic network from October 2020 to July 2022. To have a better historical picture of the seismic activity, especially since the dam construction and initial operations, re-processing of the old seismological catalogs was carried out. This required digitizing the paper-only catalog copies prior to relocation. We finally obtain a uniform catalog for the Enguri region to characterize the seismicity and start investigating its possible relationship with the exploitation of the dam reservoirs.