bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the Cyclization of Non‐cyclic Peptides for Biological Applications: Inspiration from Naturally Cyclic Peptides
Authors: Moulahoum Hichem , Ghorbanizamani Faezeh , Tok Kerem , Zihnioglu Figen 
Published: October 2023

AbstractPeptides are unique class of biomolecules for pharmaceutics and industries, given their structural features that can be applied to many approaches. Although their advantages are known, they suffer from some limitations that need to be overcome. Some disadvantages are peptidic conformations’ flexibility and susceptibility to proteolytic degradation. Research has been in a constant endeavor to provide solutions. The discovery of cyclic peptides in plants opened the door for new insights into peptide‐based applications. These peptides display high stability to physical and chemical conditions. They possess a wide range of biological activities. In addition, cyclic peptides shows enhanced activities compared to linear peptides. Thus, the idea of non‐cyclic peptide cyclization can be of great use in eliminating issues and improving peptide capabilities. Inspired by the naturally found cyclic peptides, many approaches for synthetic cyclization have been proposed. The current review provides an overall discussion of the available methods for cyclization, applications, and characterization techniques. The present review offers a unique source for colleagues newly exposed to the subject and on the verge of entering the field of cyclic peptides by providing an initiating step covering the essential points needed to be considered around peptide cyclization.
Zn/Mn co‐doped NiO synthesized by sol‐gel route for the photocatalytic degradation of methylene blue dye
Authors: Ata Sadia , Zaheer Warda , Tabassum Anila , Ul Mohsin Ijaz , Altaf Imran , ur Rehman Aziz , Al Huwayz Maryam , Alwadai Norah , Nazir Arif , Iqbal Munawar 
Published: October 2023

AbstractThis work emphasis on the synthesis of Ni0.2Mn0.02Zn0.02O nanoparticles by facile sol‐gel method. The photocatalytic activity was studied for the degradation of methylene blue (MB) dye under the UV light as a function of dye initial concentration, catalyst dose and pH of the medium. Scanning electron microscopy (SEM) and energy dispersive X‐ray (EDX) were utilized for the study of morphological and elemental composition, while XRD analysis was employed for structural study of the prepared material, which formation the phase purity and size was in 20–24 nm range. The bandgap of Zn/Mn co‐doped NiO NPs (3.24 eV) was reduced as compared to pure NiO NPs (3.43 eV). The degradation efficiency of pure NiO NPs and Zn/Mn co‐doped NiO NPs was found to be 70 % (60 min) and 94 % at optimum conditions. The dielectric properties were also studied, which were also improved in response of doping. The findings revealed that the optical and catalytic properties of Zn/Mn co‐doped NiO NPs were improved versus pure NiO NPs, which could be employed for the remediation of dyes in effluents.
Photocatalyst‐Free Visible‐Light‐Promoted C‐3‐Alkylation of Indoles through C−H Functionalizations of N,N‐Dialkylanilines
Authors: Saikia B. Shriya , Deb Mohit L. , Baruah Pranjal K. 
Published: October 2023

AbstractVisible‐light, an environmentally benign and sustainable energy source, is considered an ideal condition for green chemical synthesis especially through C−H functionalization which has been a major focus for the synthesis of organic molecules in the last two decades. Here, we report a visible‐light driven photocatalyst‐free C‐3‐alkylation of indoles via C−H functionalizations of N,N‐dialkylanilines. When N,N‐dialkylaniline is reacted with various substituted indoles in DMSO solvent in an open reaction vessel at room temperature (r.t.) under the irradiation of a Compact Fluorescent Light (CFL, 27 W), C‐3‐alkylation of indoles occurs. The yield of the resulting product is up to 96 %. This photochemical transformation offers several advantages such as catalyst‐free, aerial oxygen as the sole oxidant, visible‐light promoted, and tolerable for various functional groups in indoles.
K2S2O8‐Mediated 1,2‐Hydroxycarbonylation of Alkenes to Construct Hydroxyl‐Functionalized Chroman‐4‐Ones
Authors: Sun Yong‐Bin , Luo Chun‐Mei , Wang Ling‐Tao , Li Long , Zhang Can‐Can , Ge Guo‐Ping , Liu Hongxin , Wei Wen‐Ting 
Published: October 2023

AbstractDifunctionalization of alkenes allows the rapid construction of complex molecules with high selectivity and efficiency in organic synthesis. Herein, we first describe a 1,2‐hydroxycarbonylation of alkenes for the preparation of hydroxyl‐functionalized chroman‐4‐ones through the cooperative action of inorganic oxidants and organic acids. The transformation is initiated by the formation of an acyl radical, and then experiences intramolecular 6‐exo‐trig radical cyclization and hydroxylation process. It is notable that the hydroxyl group in the final products originate from organic acids without the use of any transition‐metal catalysts. To further demonstrate the practicality and mechanism of this method, the gram‐scale reaction and a series of control experiments including gas chromatography‐mass spectrometry (GC‐MS) and infrared (IR) absorption spectroscopy experiments were performed, and the corresponding intermediates were successfully captured.
RETRACTED: DNMT1‐mediated regulating on FBXO32 promotes the progression of glioma cells through the regulation of SKP1 activity
Authors: Quan Junjie , Ma Chengwen 
Published: October 2023

AbstractGlioma, a prevalent and serious form of brain cancer, is associated with dysregulation of DNA methylation, where DNA methyltransferase‐1 (DNMT1) plays a significant role in glioma progression. However, the involvement of F‐box protein 32 (FBXO32) in glioma and its regulation by DNMT1‐mediated methylation remain poorly understood. In this study, we investigated FBXO32 expression in glioma cells with high DNMT1 expression using the online dataset and correlated it with patient survival. Then impact of elevated FBXO32 expression on cell proliferation, migration, and invasion was evaluated, along with the examination of EMT‐related proteins. Furthermore, a xenograft model established by injecting glioma cells stably transfected with FBXO32 was used to evaluate tumor growth, volume, and weight. The ChIP assay was employed to study the interaction between DNMT1 and the FBXO32 promoter, revealing that DNMT1 negatively correlated with FBXO32 expression in glioma cells and promoted FBXO32 promoter methylation. Moreover, we investigated the interaction between FBXO32 and SKP1 using Co‐IP and GST pulldown assays, discovering that FBXO32 acts as an E3 ubiquitin ligase and promotes SKP1 ubiquitination, leading to its degradation. Interestingly, our findings demonstrated that high FBXO32 expression was associated with improved overall survival in glioma patients. Knockdown of DNMT1 in glioma cells increased FBXO32 expression and suppressed malignant phenotypes, suggesting that FBXO32 functions as a tumor suppressor in glioma. In conclusion, this study reveals a novel regulatory mechanism involving DNMT1‐mediated FBXO32 expression in glioma cells, where FBXO32 acts as an E3 ubiquitin ligase to degrade SKP1 via ubiquitination. This FBXO32‐mediated regulation of SKP1 activity contributes to the progression of glioma cells. These findings provide important insights into the molecular mechanisms underlying glioma progression and may hold promise for the development of targeted therapies for glioma patients.
LINC01485 contributes to colorectal cancer progression by targeting miR‐383‐5p/KRT80 axis
Authors: Gao Xia , Wang Guangxin , Zhang Min , Zhang Xinxin , Zhang Shuosheng , Long Haocheng 
Published: October 2023

AbstractLong non‐coding RNAs (lncRNAs) are important in tumorigenesis and the development of multiple malignant human tumors, including colorectal cancer (CRC). We aimed to determine the regulatory mechanism of LINC01485 and its biological function in CRC. We estimated the expression of miR‐383‐5p, KRT80, and LINC01485 in CRC cells and tissues using quantitative reverse transcription polymerase chain reaction (qRT‐PCR) and western blotting. The results were confirmed using RNA immunoprecipitation (RIP) and dual‐luciferase assays. Binding relationships among miR‐383‐5p, LINC01485, and KRT80 were assessed. We explored the molecular mechanisms and functions of the LINC01485/miR‐383‐5p/KRT80 axis using CCK‐8 and colony formation assays. Expression of the apoptotic markers Bcl‐2 and Bax was quantified by western blotting, and the effects of LINC01485 on tumor development in vivo were investigated using xenograft tumors. Both LINC01485 and KRT80 were upregulated, whereas miR‐383‐5p was downregulated in CRC cells and tissues. Knockdown of LINC01485 attenuated CRC cell growth and xenograft tumor formation in vivo, whereas LINC01485 enhanced the proliferative capacity of CRC cells but inhibited apoptosis by sponging miR‐383‐5p to increase KRT80 expression in CRC cells. The regulatory molecular mechanism of the LINC01485/miR‐383‐5p/KRT80 axis plays a crucial role in CRC progression. Our findings highlight novel pathways and promising biomarkers for diagnostic and therapeutic application to patients with CRC.
Temporal analysis of lung injury induced by real‐ambient PM2.5 exposure in mice
Authors: Zeng Huixian , Chen Wei , Li Meizhen , Shao Yueting , Li Xun , Zhang Rong , Jiang Yiguo 
Published: October 2023

AbstractFine particulate matter (PM2.5) has been shown to induce lung injury. However, the pathophysiological mechanisms of PM2.5‐induced pulmonary injury after different exposure times are poorly understood. In this study, we exposed male ICR mice to a whole‐body PM2.5 inhalation system at daily mean concentration range from 92.00 to 862.00 μg/m3 for 30, 60, and 90 days. We found that following prolonged exposure to PM2.5, pulmonary injury was increasingly evident with significant histopathological alterations. Notably, the pulmonary inflammatory response and fibrosis caused by PM2.5 after different exposure times were closely associated with histopathological changes. In addition, PM2.5 exposure caused oxidative stress, DNA damage and impairment of DNA repair in a time‐dependent manner in the lung. Importantly, exposure to PM2.5 eventually caused apoptosis in the lung through upregulation of cleaved‐caspase‐3 and downregulation of Bcl‐2. Overall, our data demonstrated that PM2.5 led to pulmonary injury in a time‐dependent manner via upregulation of proinflammatory and fibrosis‐related genes, and activation of the DNA damage response. Our findings provided a novel perspective on the pathophysiology of respiratory diseases caused by airborne pollution.
Überprüfung visueller Defizite durch die Polizei bei Verkehrsteilnehmern in Deutschland
Authors: Bartram M. C. , Kanngießer J. , Hufendiek K. , Schalhorn C. , Framme C. 
Published: October 2023

Zusammenfassung
Hintergrund
Der Berufsverband der Augenärzte (BVA) schätzt, dass auf deutschen Straßen mindestens hunderttausende Verkehrsunfälle mit zehntausenden Verletzten und Toten jährlich durch bekannte und nicht-bekannte Störungen des Sehens verursacht werden. Die Polizei hatte bisher allerdings nicht die Möglichkeit, bei fahrauffälligen Personen potenzielle Sehstörungen vor Ort zu prüfen. In einem Pilotprojekt der Polizei mit der Augenklinik der Medizinischen Hochschule Hannover (MHH) wurde erstmals darauf abgezielt, die bisherigen Tests der Polizei durch angepasste „Car-side“-Sehprüfungen zu erweitern.

Material und Methode
Vor Ort werden die Augenmotilität, die Pupillengröße und die Lichtreaktion von Autofahrern evaluiert sowie eine orientierende Sehschärfenprüfung und ein orientierendes Konfrontationsgesichtsfeld durchgeführt. Die praktische Durchführung sämtlicher Untersuchungsmodalitäten wurde zuvor abgestimmt und trainiert.

Ergebnisse
Umfang und Art der einzelnen Untersuchungen zur Abschätzung der Sehfähigkeit am PKW wurden beschrieben und standardisiert. Neben den visuellen Merkmalen werden auch bekanntere neurologische Tests, wie z. B. „Gerade auf einer Linie laufen“, erfasst. Durch die kombinierte Testauswertung ergibt sich eine objektive Grundlage zur behördlichen Entscheidung einer ggf. notwendigen sofortigen Führerscheinsicherstellung und zu dem Einleiten weiterer Maßnahmen wie beispielsweise einer augenärztlichen Überprüfung. Mit diesen Neuerungen hat die Polizei Niedersachsen ihr Ausbildungskonzept zur Überprüfung der Fahrtüchtigkeit überarbeitet und Anfang 2022 inklusive der augenärztlicherseits empfohlenen Sehprüfungen zur Umsetzung herausgegeben. Mittlerweile sind in Niedersachsen rund 150 Beamte ausgebildet worden und haben jetzt die Berechtigung „qualifizierte Fahrtüchtigkeitsprüfungen“ durchzuführen. Diesbezüglich wurde bereits eine Vielzahl von entsprechenden Verkehrsprüfungen durchgeführt, und die Weiterfahrten von Verkehrsteilnehmern mit erheblichen visuellen Defiziten wurden unterbunden.

Schlussfolgerung
In diesem Pilotprojekt wurde erstmals darauf abgezielt, die gängigen Testverfahren der (Verkehrs‑)Polizei zur Erkennung von verkehrsgefährdenden Autofahrern neben Screening auf Alkohol, Drogen und neurologische Defizite um spezifische Tests zur Erkennung von visuellen Defiziten zu erweitern. Das entsprechende Training der Polizeibeamten wurde in Niedersachsen und Hamburg flächendeckend durchgeführt, und die Maßnahmen finden aktuell bei Verkehrskontrollen im Bedarfsfall Anwendung. Die überprüften Merkmale sollen zukünftig auch in einem gewichteten Score bewertet werden und eine objektive Entscheidungsgrundlage für die Polizei darstellen, bei entsprechenden Defiziten ggf. eine Weiterfahrt zu unterbinden.
Combined associations of a healthy lifestyle and body mass index with colorectal cancer recurrence and survival: a cohort study
Authors: Barot Shabane , Rantanen Petri , Nordenvall Caroline , Lindforss Ulrik , Hallqvist Everhov Åsa , Larsson Susanna C. , Lindblom Annika , Liljegren Annelie 
Published: October 2023

Abstract
Purpose
Colorectal cancer (CRC) risk is associated with modifiable lifestyle factors including smoking, physical inactivity, Western diet, and excess body weight. The impact of lifestyle factors on survival is less known. A cohort study was conducted to investigate the combined effects of a healthy lifestyle and body mass index on prognosis following CRC diagnosis.

Methods
Treatment and follow-up data were collected from the patient files of 1098 participants from the Colorectal cancer low-risk study cohort including stage I-III CRC patients. A healthy lifestyle and BMI (HL) score was computed using self-reported data on smoking status, physical activity, adherence to a Mediterranean diet pattern, and BMI, and divided into four categories ranging from least to most healthy. Survival analyses were performed to assess recurrence-free survival and overall survival across categories of exposure, using the Kaplan–Meier method and Cox proportional hazards models adjusted for age, sex, and educational level.

Results
Among 1098 participants with stage I-III CRC, 233 (21.2%) had an HL score of 0–1 (least healthy), 354 (32.2%) HL score of 2, 357 (32.5%) HL score of 3 and 154 (14.0) HL score 4 (most healthy). Patients with the healthiest lifestyle (HL score 4) compared to the least healthy (HL score 0–1) had an improved recurrence-free survival (HL 4 vs HL 0–1, HRadj 0.51 (95% CI 0.31–0.83) and overall survival (HL 4 vs HL 0–1, HRadj 0.52 (95% CI 0.38–0.70).

Conclusion
Adherence to a healthy lifestyle may increase the recurrence-free and overall survival of patients with stage I–III CRC.
Trace metal content prediction along an AMD (acid mine drainage)-contaminated stream draining a coal mine using VNIR–SWIR spectroscopy
Authors: Abrahams Jamie-Leigh Robin , Carranza Emmanuel John M. 
Published: October 2023

AbstractThe current study investigated the use of VNIR–SWIR (visible/near infrared to short-wavelength infrared: 400–2500 nm) spectroscopy for predicting trace metals in overbank sediments collected in the study site. Here, we (i) derived spectral absorption feature parameters (SAFPs) from measured ground spectra for correlation with trace metal (Pb, Cd, As, and Cu) contents in overbank sediments, (ii) built univariate regression models to predict trace metal concentrations using the SAFPs, and (iii) evaluated the predictive capacities of the regression models. The derived SAFPs associated with goethite in overbank sediments were Depth433b, Asym433b, and Width433b, and those associated with kaolinite in overbank sediments were Depth1366b, Asym1366b, Width1366b, Depth2208b, Asym2208b, and Width2208b. Cadmium in the overbank sediments showed the strongest correlations with the goethite-related SAFPs, whereas Pb, As, and Cu showed strong correlations with goethite- and kaolinite-related SAFPs. The best predictive models were obtained for Cu (R2 = 0.73, SEE = 0.15) and Pb (R2 = 0.73, SEE = 0.21), while weaker models were obtained for As (R2 = 0.46, SEE = 0.31) and Cd (R2 = 0.17, SEE = 0.81). The results suggest that trace metals can be predicted indirectly using the SAFPs associated with goethite and kaolinite. This is an important benefit of VNIR–SWIR spectroscopy considering the difficulty in analyzing “trace” metal concentrations, on large scales, using conventional geochemical methods.
The Influence of Bidirectional Intimate Partner Violence on the Parent-child Relationship
Authors: Vasconcelos Diana , Machado Andreia , Caridade Sónia , Cunha Olga 
Published: October 2023

Abstract
Purpose
Intimate partner violence (IPV) is a public health issue and a human rights violation. Recent studies have revealed that most IPV is bidirectional rather than unidirectional, which can result in serious consequences for those involved. However, the impact of bidirectional violence (BV) on parenting, and more specifically on the parent-child relationship, is understudied. Thus, this study aims to understand the impact of BV on the parent-child relationship and analyze the differences in the perceptions about the quality of the parent-child relationship between parents involved in unidirectional IPV, parents involved in bidirectional IPV, and parents without IPV.

Methods
The sample comprised 138 participants of both sexes, aged 18 years or over, and with underage children. The study was conducted online, and data were collected through a sociodemographic questionnaire, the Inventory of Marital Violence, and the Inventory of Parent-Child Relationship.

Results
Parents (male and female) involved in bidirectional IPV reported that the communication dimension was the most impacted and presented perceptions of lower quality in the relationship with their children than parents involved in unidirectional IPV perpetration.

Conclusions
These results suggest that individuals involved in BV might transpose the dynamics they establish in their marital relationship to their relationship with their children, resulting in less positive parent-child relationships. A new perspective on victim and perpetrator profiles emerges from this study, which should be considered regarding parenting intervention.
Effects of Si and SO2 on Corrosion of Fe–20Cr and Fe–20Cr–20Ni Alloys in Reducing Waste Combustion Gases
Authors: Nguyen Thuan Dinh , Zhang Jianqiang , Young David J. 
Published: October 2023

AbstractModel alloys, Fe–20Cr and Fe–20Cr–20Ni (wt%) with and without 1% Si, were exposed to four different gas mixtures Ar–10H2O, Ar–10H2O–0.1HCl, Ar–10H2O–0.1HCl–10CO2, Ar–10H2O–0.1HCl–10CO2–0.5SO2 (vol.%) at 650 °C. The Fe–20Cr and Fe–20Cr–20Ni alloys underwent breakaway corrosion in Ar–10H2O–(0.1HCl)–(10CO2), forming Cr2O3 and Fe-rich oxide nodules. Silicon addition markedly increased corrosion resistance for both alloys by forming an additional SiO2 layer beneath the Cr2O3 scale. Addition of SO2 to Ar–10H2O–0.1HCl–10CO2 significantly suppressed the formation of Fe-rich oxide nodules on Fe–20Cr. Corrosion in the SO2-bearing gas resulted in (Cr, Fe, S)-rich precipitates in all alloys. The effects of gas composition and Si alloying on corrosion of both alloys are discussed in terms of transport processes within the scales.
Pulse and CW EPR Oximetry Using Oxychip in Gemcitabine-Treated Murine Pancreatic Tumors
Authors: Dziurman Gabriela , Drzał Agnieszka , Murzyn Aleksandra Anna , Kmiec Maciej Mikolaj , Elas Martyna , Krzykawska-Serda Martyna 
Published: October 2023

Abstract
Purpose
The goal of this work was to compare pO2 measured using both continuous wave (CW) and pulse electron paramagnetic resonance (EPR) spectroscopy. The Oxychip particle spin probe enabled longitudinal monitoring of pO2 in murine pancreatic tumor treated with gemcitabine during the course of therapy.

Procedures
Pancreatic PanO2 tumors were growing in the syngeneic mice, in the leg. Five doses of saline in control animals or gemcitabine were administered every 3 days, and pO2 was measured after each dose at several time points. Oxygen partial pressure was determined from the linewidth of the CW EPR signal (Bruker E540L) or from the T2 measured using the electron spin echo sequence (Jiva-25™).

Results
The oxygen sensitivity was determined from a calibration curve as 6.1 mG/mm Hg in CW EPR and 68.5 ms−1/mm Hg in pulse EPR. A slight increase in pO2 of up to 20 mm Hg was observed after the third dose of gemcitabine compared to the control. The maximum delta pO2 during the therapy correlated with better survival.

Conclusions
Both techniques offer fast and reliable oximetry in vivo, allowing to follow the effects of pharmaceutic intervention.
Reconsidering the Relationship Between Anti-immigration Attitudes and Preferences for the AfD Using Implicit Attitudes Measures
Authors: Kleinert Manuel 
Published: October 2023

AbstractThe study of demand-side factors for the success of radical right-wing populist parties has highlighted anti-immigration attitudes (AIA) as a particularly important predictor. However, these findings have relied heavily on direct self-report measures. This preregistered study theorises that direct measures may have underestimated, through social desirability bias, or overestimated, through cognitive dissonance avoidance, the relationship between AIA and support for the German radical right-wing party Alternative for Germany (AfD). A direct questionnaire and two Single-Category Implicit Association Tests were administered to a stratified sample of the German population (N = 369) to measure both explicit and implicit preferences for the AfD and AIA. Results reveal that the firm relationship between AIA and AfD voting intentions is strongest in an all-explicit setting, reduced in mixed analyses, and eliminated in the all-implicit model. This provides evidence that the need for respondents to report consistent ideologies may be a more serious threat to valid results in political attitudes research than is generally assumed. Social desirability seems to be less of an issue when assessing the strength of the correlation between right-wing attitudes and AfD preferences. Thorough robustness checks confirmed the reliability of these findings.