bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal relationship between diet and celiac disease in leukemia: A two-sample Mendelian randomization study
Authors: cheng Shupeng , Zhang Meiling , Luo Junyun , Zheng Hang , Wu Zhenhui , Tang Nana , Zeng Yingjian 
Published: November 2023

Abstract
Objective To explore the causal relationship between celiac disease and a wheat-free diet with leukemia.
Methods We obtained genome-wide association study (GWAS) data on celiac disease, a wheat-free diet, and leukemia from published literature. Single nucleotide polymorphisms (SNPs) representing celiac disease or a wheat-free diet were selected as instrumental variables. Two-sample Mendelian randomization (MR) analyses were conducted using inverse-variance weighted (IVW), weighted median, MR-Egger regression, simple mode, and weighted mode methods. The reliability of the results was evaluated using MR-Egger test, MR-PRESSO test, Cochran Q test, and leave-one-out test.
Results We identified 16 SNPs associated with celiac disease as instrumental variables and 5 SNPs associated with a wheat-free diet as instrumental variables. MR analysis showed that an increase in celiac disease is associated with an increased risk of leukemia (OR = 1.03, 95% CI: 1.01-1.07, P = 0.03) according to the IVW method, and similar results were obtained using the MR-Egger regression (OR = 1.04, 95% CI: 1.00-1.09, P = 0.04). Consistent causal effects were observed using the five methods. The IVW method revealed that a wheat-free diet in healthy individuals leads to an increased risk of leukemia (OR = 1.06, 95% CI: 1.03-1.08, P = 2.36E-05), and similar results were obtained using the MR-Egger regression (OR = 1.06, 95% CI: 0.98-1.15, P = 0.27). The five methods consistently supported these causal directions. Reliability analysis indicated no heterogeneity, no pleiotropy, and no significant bias in both sets of MR analyses, suggesting the reliability of the results.
Conclusion Celiac disease is a risk factor for leukemia, and a wheat-free diet in healthy individuals also increases the risk of developing leukemia. Adjusting one's own immune system may be the best choice for individuals with celiac disease.
Asian Low-Carbohydrate Diet with Increased Whole Egg Consumption Improves Metabolic Outcomes in Metabolic Syndrome: A 52-Week Intervention Study
Authors: Pinsawas Bonggochpass , Mongkolsucharitkul Pichanun , Pongkunakorn Tanyaporn , Surawit Apinya , Suta Sophida , Manosan Thamonwan , Ophakas Suphawan , Pumeiam Sureeporn , Sranacharoenp Kitti , Mayurasakorn Korapat 
Published: November 2023

Abstract
Background: The low-carbohydrate-ketogenic diet, an effective strategy to address metabolic syndrome (MetS) and obesity has been concerns about high fat consumption on atherogenic lipoproteins. This study aimed to compare the Asian ketogenic diet (AKD), which incorporates balanced protein and fat intake from Asian foods, with a balanced low-caloric diet (BLC) in individuals diagnosed with MetS.
Methods: A 52-week randomized clinical trial included three parallel groups: AKD with increased whole egg intake (Yolk-KD, aged 40.9 ± 1.7, n = 27), yolk-free ketogenic diet with egg white supplementation (White-KD, aged 41.5 ± 1.3, n = 26), and BLC diet (aged 38.5 ± 1.7, n = 22). Primary outcomes were anthropometric and metabolic changes.
Results: The AKD groups achieved significant reductions in weight and waist circumference (P < 0.05). Compared to the BLC group, the AKD groups demonstrated significant improvements in fasting blood glucose, insulin resistance, and lipid profile at weeks 12 and 35 (P < 0.05). All groups experienced improvements in insulin sensitivity, inflammation, and appetite-related hormones like leptin and peptide YY (P < 0.05). From weeks 35 to 52, the AKD consistently maintained reductions in anthropometric measurements, improved glucose tolerance, enhanced lipid profiles, and better liver function compared to the BLC.
Conclusion: The AKD proved safe and effective, yielding various metabolic improvements in individuals with Mets compared to the BLC. By emphasizing a low-saturated fat diet while disregarding dietary cholesterol, this approach holds promise for MetS and obesity management. Further studies are warranted.
Trial registration:ClinicalTrials.gov identifier: NCT04608136, registered on September 21, 2020.
Identification and cellular validation of the relevant potential biomarkers associated with female lung adenocarcinoma
Authors: Zhang JianPing , An XiaoQiao , Deng ChengCheng , Lui Jingyu , Liu Ying 
Published: November 2023

Abstract
Background: FOXM1 plays a pivotal role in regulating tumor progression in various cancer types. However, its involvement in the progression of female lung adenocarcinoma (LUAD) and potential impact on immunotherapy remain uncharacterized.
Methods: To investigate the role of FOXM1 in LUAD, we conducted a comprehensive analysis using GDC TCGA (Genomic Data Commons The Cancer Genome Atlas) and GEO (Gene Expression Omnibus) datasets along with a Gene Set Enrichment Analysis approach. Our methodology included differential expression analysis, survival correlation analysis, survival correlation meta-analysis, and clinical correlation analysis, all aimed at elucidating the relationship between FOXM1 expression and LUAD. Additionally, the miRDB, miRTarBase, and TargetScan databases were used to predict target microRNAs (miRNAs). Co-expression analysis was performed to explore the interplay between target miRNAs, FOXM1, target long non-coding RNAs, and the estrogen receptor. A competitive endogenous RNA (ceRNA) network was constructed using Cytoscape. The analysis of tumor mutational burden (TMB) was employed to gauge the sensitivity of FOXM1-mutated LUAD to immunotherapy. Furthermore, the Gene Set Enrichment Analysis package facilitated the examination of immune cell infiltration in LUAD samples. Finally, we employed R tools to assess the immunotherapeutic effects of LUAD. We conducted in vitro experiments to evaluate the biological role of FOXM1.
Results: FOXM1 expression was elevated in LUAD samples compared to that in normal tissues. Moreover, results from survival and clinical correlation analyses underscored the significant influence of FOXM1 expression on LUAD progression. FOXM1 knockdown has a substantial impact on LUAD cell proliferation and apoptosis. We established a ceRNA network involving DGCR-5, has-miRNA-204-5p, FOXM1, and estrogen receptor 1. Validation experiments confirmed that has-miR-204-5p is a target miRNA for FOXM1, whereas DGCR5 is not a target long non-coding RNAs for has-miR-204-5p. Furthermore, our study demonstrated a physical interaction between FOXM1 and estrogen receptors. Immune-related analyses indicated that the low FOXM1 expression group exhibited increased sensitivity to immunotherapy, including anti-PDA and anti-CTLA treatment.
Conclusion: We established a new ceRNA network (DGCR-5---has-miRNA-204-5p---FOXM1---estrogen receptor 1) that holds promise for unraveling mechanistic insights into LUAD and predicting survival outcomes in LUAD patients.
Lateral Heterostructures of WS2 and MoS2 Monolayers for Photo-Synaptic Transistor
Authors: Park Jaeseo , Kim Jun Oh , Kang Sang-Woo 
Published: November 2023

Abstract
Von Neumann architecture-based computing, while widely successful in personal computers and embedded systems, faces inherent challenges including the von Neumann bottleneck, particularly amidst the ongoing surge of data-intensive tasks. Neuromorphic computing, designed to integrate arithmetic, logic, and memory operations, has emerged as a promising solution for improving energy efficiency and performance. This approach requires the construction of an artificial synaptic device that can simultaneously perform signal processing, learning, and memory operations. We present a photo-synaptic device with 32 analog multi-states by exploiting field-effect transistors (FETs) based on the lateral heterostructures of two-dimensional (2D) WS2 and MoS2 monolayers, formed through a two-step metal-organic chemical vapor deposition process. These lateral heterostructures offer high photoresponsivity and enhanced efficiency of charge trapping at the interface between the heterostructures and SiO2 due to the presence of the WS2 monolayer with large trap densities. As a result, it enables the photo-synaptic transistor to implement synaptic behaviors of long-term plasticity and high recognition accuracy. To confirm the feasibility of the photo-synapse, we investigated its synaptic characteristics under optical and electrical stimuli, including the retention of excitatory post-synaptic currents, potentiation, habituation, nonlinearity factor, and paired-pulse facilitation. Our findings suggest the potential of versatile 2D material-synapse with a high density of device integration.
Fluorescent probes based on Ag NPs@N/GQDs and molecularly imprinted polymer for sensitive detection of noradrenaline in bananas
Authors: Wang Yaru , Li Shuhuai , Ma Xionghui , Pang Chaohai , Wu Yuwei , Wang Mingyue , Li Bei , Liu Sixin 
Published: November 2023

Abstract
Fluorescence intensity and selective recognition ability are crucial factors in determining the analytical techniques for fluorescent probes. In this study, a core-shell fluorescent material composed of silver nanoparticles@nitrogen-doped graphene quantum dots (Ag NPs@N/GQDs) was synthesised using mango leaves as the raw material through a thermal cracking method, resulting in strong fluorescence luminescence intensity. By employing noradrenaline as a template molecule and utilising a surface molecular imprinting technique, a molecularly imprinted membrane (MIP) was formed on the surface of the fluorescent material, which was subsequently eluted to obtain a highly specific fluorescent probe capable of recognising noradrenaline. The probe captured various concentrations of noradrenaline using the MIP, causing a decrease in the probe fluorescence intensity. Then a method for detecting trace amounts of noradrenaline was established. This method exhibited a linear range from 0.5 –700 pM with a detection limit of 0.154 pM. The proposed method was achievemently implemented in banana samples. Satisfactory recoveries were confirmed at four different concentrations. The method presented a relative standard deviation (RSD%) of less than 5.0%.
Introgression of genes associated with yield enhancement and resistance against bacterial leaf blight and blast diseases into an elite rice variety, ‘Jaya’ through marker assisted backcrossing
Authors: GD Dileep Kumar , R ABDUL Fiyaz , Kasarla Chaithanya , Viswanatha Kannalli Paramashivaiah , Darmagaru Shivani , Patwari Payal , Bupalli Sathwik , M.B.V.N Kousik , VS Rapaka Percy , M Bharath Kumar , More Sahithi , KS Raghuwanshi , Chimote Vivek Pandurang , GS Laha , J Aravind Kumar , Basavaraj K , Rao Lella Venkata Subba , Sundaram Raman Meenakshi 
Published: November 2023

Abstract
Background
Bacterial leaf blight (BLB) and blast diseases are the major biotic threats responsible for significant yield loss in rice production across all the rice growing regions. Development of high yielding lines along with resistance to major rice disease is more relevant solution for sustainable yields in paddy. ‘Jaya’ the most popular, stable mega rice variety, notified and cultivated in nineteen states across India and is susceptible to bacterial leaf blight and blast diseases and has an average yielding ability (4.5 t/ha). Present study targeting introgression of bacterial leaf blight, blast resistance genes along with yield improvement genes is the most felicitous strategy for a panoramic genetic enhancement to develop high yielding disease resistant rice cultivars.
Results
In the current investigation, efforts have been made to transfer ‘Xa21’ gene which provide resistance to bacterial leaf blight caused by Xanthomonas oryzae pv oryzae, ‘Pi54’ gene which governs blast resistance caused by Magnoporthe oryzae and yield enhancing gene ‘Gn1a’ which governs the higher grain number per panicle thereby ultimately increasing the yield of the concerned genotype. Using marker assisted backcross breeding approach twenty elite BC2F2 lines with two biotic stress resistance genes (Xa21 and Pi54) and a yield enhancement gene (Gn1a) were developed and evaluated for their disease resistance, yield and agronomic performance under field and controlled condition in BC2F3 generation. The background genome recovery analysis of twenty elite BC2F2 lines was carried out using 1K-RiCA SNP genotyping and it ranged from 85.41 to 96.28 per cent. The selected backcross derived lines showed higher resistant to BLB and blast along with significant yield increase over recurrent parent.
Conclusions
The strategy of marker assisted backcross selection found to be promising technique in identifying superior backcross introgressed lines. Among the twenty improved backcross derived lines, IJ-4 and IJ-107 were found to be more promising lines by having the highest recurrent parent genome recovery along with increased resistance to BLB and blast with superior yielding ability than recurrent parent Jaya.
Comparison of feasibility and effectiveness of catheter placement with or without DSA guidance: a propensity score-matched cohort study
Authors: Shang Yiwei , Pan Shujun , Jin Chen , Zheng Danna , Xu Xiujun , Zhu Bin , Zhao Li , Jin Juan , He Qiang , Shen Xiaogang 
Published: November 2023

Abstract
Background
The use of digital subtraction angiography (DSA)-guided placement for tunneled dialysis catheter (TDC) placement is preferred in developed countries due to its higher success rate and accurate positioning. However, in developing countries with limited medical resources, the procedure is typically only performed under ultrasound guidance. The healthcare system is facing challenges due to the shortage of resources and financial pressures. This study aims to evaluate the feasibility and effectiveness of DSA-guided catheter placement compared to ultrasound-guided placement in developing countries.
Methods
This retrospective cohort study included all TDC insertions at a hospital between January 2020 and October 2022. Patient demographics, laboratory results, surgical details, and hemodialysis information were collected. Propensity score matching (PSM) was used to balance the demographics and clinical characteristics between the DSA-guided and ultrasound-guided groups. Adverse events, catheter function, catheter tip position, and costs were evaluated.
Results
After matching, there were no significant differences between the groups. Both groups achieved adequate catheter blood flow and ultrafiltration volumes without deviations from dialysis prescriptions. The DSA-guided group had fewer events leading to early termination of dialysis compared to the ultrasound-guided group. Catheter tip position in the right atrium was more consistent in the DSA-guided group. Cost analysis showed higher expenses with DSA-guided procedures. When the study was limited to patients with catheter tips located in the right atrium, there was no significant difference in adverse events between the two groups.
Conclusion
The performance of hemodialysis catheters inserted under DSA guidance is superior to those inserted under ultrasound guidance during postoperative blood dialysis. This may be related to the position of the tip of the catheter. We recommend using DSA when it cannot be ensured that the tip of the catheter is located in the right atrium.
Low dose methotrexate impaired T cell transmigration through down-regulating CXCR4 expression in rheumatoid arthritis
Authors: Ding Lei , Park Daniel , Gao Bo , Wu Lingyuan , Li Meizhang , Abedelhakim Haitham , Zhang Ming 
Published: November 2023

Abstract

Background
CXC chemokine CXCL12 is involved in the pathological development of rheumatoid arthritis (RA) through abnormal migration of peripheral immune cells in the joint. Although low dose methotrexate (MTX) is clinically used to treat RA patients, CXCL12 signaling responses to MTX-mediated treatments is still controversial.
Methods
In this study, we examined the expression of CXCR4 (cognatic receptor for CXCL12) in peripheral T cells from RA patients and arthritis mice models received from low dose MTX therapies. The effects of low dose MTX on CXCR4 was further determined via both in vitro CD3 + T cells and Cxcr4 conditional knockout (CKO) arthritis mice models.
Results
Our clinical data shows that low dose MTX treatment was clinically associated with down-regulated expression of chemokine receptor CXCR4 on patient peripheral T cells. In vitro, low dose MTX significantly decreased cell transmigration through down-regulated CXCR4’s expression in CD3 + T cells. Consistently, CD3 + T cells treated with low dose MTX demonstrated an increased genomic hypermethylation across the promoter region of Cxcr4 gene. Furthermore, our preclinical studies showed that low dose MTX-mediated downregulation of CXCR4 significantly improved the pathological development of mouse arthritis models. Conditional disruption of the Cxcr4 gene in peripheral immune cells potentially alleviated inflammation of joints and lung tissue in the arthritis mice, though genetic modification itself did not change their clinical scores of arthritis.
Conclusion
Our findings suggest that the effect of low dose MTX treatment could serve to eliminate inflammation in RA patients through impairment of immune cell transmigration.
Optimizing Solid Waste Collection and route using POA algorithm
Authors: Son Pham Vu Hong , Van Tran Thiet 
Published: November 2023

Abstract
The study explores the intricacies of Municipal Solid Waste (MSW) collection in the urban context where its high per capita waste generation and susceptibility to the adverse impacts of climate change, particularly in developing countries. This research focuses on a specialized vehicle routing model to optimize the solid waste transportation system within the city. To enhance the model's efficiency, a novel combined method is proposed, integrating the Pelican Optimization Algorithm (POA). Experimental evaluations, conducted using real data, unequivocally demonstrate that the proposed combined method outperforms alternative approaches, including the current manual MSW collection protocol in the city. This research makes a significant contribution to the waste management and urban sustainability field, with the ultimate goal of mitigating the social, economic, and environmental consequences associated with urban waste collection.
Mir155hg accelerates hippocampal neuron injury in convulsive status epilepticus by inhibiting microglial phagocytosis
Authors: Wang Ming , Xu Binyuan , Xie Yangmei , Yao Ge , Chen Yinghui 
Published: November 2023

Abstract
Convulsive status epilepticus (CSE) is a common critical neurological condition that can lead to irreversible hippocampal neuron damage and cognitive dysfunction. Multiple studies have demonstrated the critical roles that long non-coding RNA (lncRNA) Mir155hg plays in a variety of diseases. However, less is known about the function and mechanism of Mir155hg in CSE. Here we investigate and elucidate the mechanism underlying the contribution of Mir155hg to CSE-induced hippocampal neuron injury. By applying high-throughput sequencing, we examined the expression of differentially expressed genes in normal and CSE rats. Subsequent RT-qPCR enabled us to measure the level of Mir155hg in rat hippocampal tissue. Targeted knockdown of Mir155hg was achieved by the AAV9 virus. Additionally, we utilized HE and Tunel staining to evaluate neuronal injury. Immunofluorescence (IF), Golgi staining, and brain path clamping were also used to detect the synaptic plasticity of hippocampal neurons. Finally, through IF staining and Sholl analysis, we assessed the degree of microglial phagocytic function. It was found that the expression of Mir155hg was elevated in CSE rats. HE and Tunel staining results showed that Mir155hg knockdown suppressed the hippocampal neuron loss and apoptosis followed CSE. IF, Golgi staining and brain path clamp data found that Mir155hg knockdown enhanced neuronal synaptic plasticity. The results from IF staining and Sholl analysis showed that Mir155hg knockdown enhanced microglial phagocytosis. Our findings suggest that Mir155hg promotes CSE-induced hippocampal neuron injury by inhibiting microglial phagocytosis.
Blended care intervention for cancer aftercare in general practice centres: a study protocol for a randomised controlled trial
Authors: Smits Michelle , Bolman Catherine , Mesters Ilse , Lechner Lilian 
Published: November 2023

Abstract
Background Combining effective eHealth programs with face-to-face consultations in general practice may help general practitioners (GPs) to care for cancer survivors.
Method This study protocol describes a two-armed randomised controlled trial (RCT) to evaluate (cost-)effectiveness of a blended intervention integrating the Cancer Aftercare Guide (CAG) in general practice centres (GPCs). A process evaluation is also included in the study.
Discussion Results may be relevant to cancer aftercare, general practice, and the field of eHealth implementation in general. Potential challenges lie in recruitment due to the strain on the healthcare system since the COVID-19 pandemic.
Trial registration The study is ongoing, patients are currently being enrolled. Enrolment started 11 August, 2023. A total of 86 patients have enrolled as of 22 November 2023.
The role of prejudice and prior contact in support for evidence-based interventions to reduce drug-related deaths: Results from a survey across the island of Ireland.
Authors: Miller Nicole M , Campbell Claire , Shorter Gillian W 
Published: November 2023

Abstract
Background: Stigmatized attitudes, opinions on opioid use, and prior contact with people who use drugs (PWUD) can contribute to what policies are supported and implemented to reduce drug-related deaths. This study examined how these variables relate to policy support for implementation of supervised injection sites, laws protecting bystander at the scene of an overdose, and over the counter naloxone on the island of Ireland.
Methods: A sample of 472 adults across the Island of Ireland completed an online survey. Hierarchical linear regression was performed to examine associations between respondent attitudes and policy support. Mediation analysis explored how stigma mediated the relationship between prior contact and policy support. Open-ended questions explored attitudes further and were analysed using codebook Thematic Analysis.
Results: The final model accounted for 29% of the variance in policy support. Non sympathetic attitudes towards people who use opioids predicted less policy support (β=-.18) and agreement that PWUD were not criminals predicted more policy support (β=.14). Mediation analysis showed that medium or high levels of familiarity with PWUD (compared to low) reduced social stigma, avoidance, and disgust which in turn increased levels of policy support. Medium familiarity (compared to low) showed a small indirect effect via sympathetic attitudes and condemnation. Meta inferences from the qualitative analysis evidenced mechanisms of stigma and prior contact.
Conclusion: Stigma reduction programs should consider the role of prejudice and target negative emotional reactions such as lack of sympathy towards PWUD. Programs to reduce stigma should be informed by the lived or living experience of PWUD, families and carers, to help increase knowledge, social acceptance and understanding to garner policy support for evidence-based intervention to reduce drug-related deaths.