bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Silico Prediction of Binding Affinities of Hybrid Molecules of Benzothiazole cross-linked with Hydroxamic acid by certain linkers
Authors: Alqaysi Yazen , Shakir M. Alwan  , Ashour H. Dawood  
Published: February 2024

New hybrid molecules of Benzothiazole cross-linked with hydroxamic acid through an amino acid or aminoalkanoic acid are suggested. All the synthesized hybrid molecules (2A-E) were subjected to molecular docking to evaluate their binding affinities with histone deacetylase enzyme (HDAC8, PDB ID: 1T69) and recorded lower ΔG (-8.117, -6.322, -8.16, -7.939, - 9.46, respectively)
than Vorinostat (suberoylanilide hydroxamic acid, SAHA), as the reference ligand, which recorded a much less value of -5.375, using the Maestro software (Schrödinger, version 2022-1). Moreover, compound 2E, which is Benzothiazole-p-amino benzoic acid-hydroxamate has recorded the lowest binding score (-9.460). This may indicate that this compound is the most active hybrid molecule. There were no violations from Lipinski’s rule and all the synthesized hybrid molecules comply with all parameters. SwissADME server was employed for the in silico molecular docking for prediction of the physicochemical and ADME properties of the investigated compounds. All hybrid molecules showed low possible passive oral absorption and no penetration into BBB. The hybrid molecules 2B and 2D may be considered as P-gp substrates. SAHA does not inhibit any of the CYP enzymes used in this study, while, the hybrid molecules 2B, 2D and 2E have shown possible inhibitory activities.
Exploring Memory Inhibition Techniques in Tan Twan Eng's The Garden of Evening Mists and Kazuo Ishiguro's The Remains of the Day
Authors: Alharbi Sarah , Alamri Dawla 
Published: February 2024

This paper offers a psychoanalytical reading of the human memory’s dilemma as portrayed by Tan Twan Eng in The Garden of Evening Mists (2011) and Kazuo Ishiguro in The Remains of the Day (1989). Its objective is to analyze the role of memory in shaping the fragmented self, particularly in the context of the trauma from the world wars, employing a psychoanalytical perspective demonstrated through Michael Anderson’s memory inhibition techniques and Carl Jung’s theories on active imagination, which prioritize the unconscious mind. The study applies two methods of selective memory to the protagonists’ behaviors: retrieval-induced forgetting in Ishiguro’s The Remains of the Day and the think/no-think paradigms in Eng’s The Garden of Evening Mists. These approaches aim to explore the role of intention in these inhibition processes and investigate whether these experiences yield positive or negative outcomes. Through a close analysis of the main characters’ repressed memories, the study argues that the protagonists employ selective memory techniques differently. In The Garden of Evening Mists, intentional and positive utilization of these techniques helps overcome the horrors of war. Conversely, in The Remains of the Day, unintentional and negative application enhances the illusion of falsely representing oneself. This study’s primary contribution lies in exploring the mechanisms underlying the control of postcolonial/imperial traumatic memories. Additionally, it delves into the significance of the natural world in the protagonists’ healing and recovery revealing that gardens serve as a metaphor for recovery due to their profound impacts.
KEPEMIMPINAN PRESIDEN MEGAWATI PADA ERA KRISIS MULTIDIMENSI, 2001-2004
Authors: Soekarnoputri Megawati 
Published: February 2024

Pada 1997 - 1998, Indonesia menghadapi berbagai macam krisis yang menimbulkan gejolak dan mengubah jalannya sejarah bangsa ini. Dimulai dari krisis ekonomi nasional, dan berlanjut pada munculnya berbagai bentuk ancaman, seperti konflik etnis dan agama, serta maraknya separatisme dan terorisme. Krisis multidimensi tersebut berpotensi menghantar Indonesia pada perpecahan dan dicap sebagai negara gagal. Penelitian ini bertujuan untuk memberi pemahaman tentang hubungan antara krisis multidimensi dan kepemimpinan presiden pada kurun waktu tahun 2001 hingga 2004. Untuk mencapai tujuan tersebut, penelitian kualitatif dengan pendekatan studi kasus dipakai untuk menggali tacit knowledge dari pengambil keputusan tertinggi saat itu, yang tidak lain adalah Presiden Republik Indonesia kelima Megawati Soekarnoputri. Penelitian ini menggunakan kerangka teori Byman dan Pollack (2001) sebagai pisau analisis dengan metode penelitian kualitatif. Hasil temuan penelitian yang diperoleh antara lain, walaupun dalam masa pemerintahan yang relatif singkat, kepemimpinan Presiden Megawati Soekarnoputri, berhasil mengatasi sebagian besar krisis multidimensi yang dihadapi oleh Indonesia saat itu. Kebijakan strategis di bidang ekonomi, politik, hukum, sosial, dan lingkungan diambil Â dengan landasan pemikiran yang inovatif, melalui berbagai upaya dialog, perundingan, pembuatan program, pengesahan peraturan perundang-undangan, hingga pemberlakuan operasi militer demi menjaga kedaulatan dan keamanan Negara Indonesia. Dampak dari berbagai kebijakan tersebut masih bisa dirasakan hingga saat ini, seperti pemilihan umum secara langsung, otonomi daerah, meletakan fondasi pembangunan kemaritiman. Keberhasilan tersebut menjadi landasan yang kokoh bagi presiden selanjutnya untuk melanjutkan program pembangunan dan peningkatan kesejahteraan rakyat di Indonesia. Salah satu kontribusi penelitian ini adalah mengeksplisitkan aspek-aspek yang melatarbelakangi pengambilan keputusan strategis pada era 2001-2004, yang diharapkan bisa berguna bagi perkembangan ilmu pengetahuan dan saran praktis dalam rangka proses penyusunan perencanaan dan kebijakan strategis pembangunan, baik pada tataran suatu negara maupun global.
Enhanced solute transport and steady mechanical stimulation in a novel dynamic perifusion bioreactor increase the efficiency of the in vitro culture of ovarian cortical tissue strips
Authors: Fragomeni Gionata , De Napoli Luigi , De Gregorio Vincenza , Genovese Vincenzo , Barbato Vincenza , Serratore Giuseppe , Morrone Giuseppe , Travaglione Angela , Candela Andrea , Gualtieri Roberto , Talevi Riccardo , Catapano Gerardo 
Published: February 2024

Introduction: We report the development and preliminary evaluation of a novel dynamic bioreactor to culture ovarian cortical tissue strips that leverages tissue response to enhanced oxygen transport and adequate mechanical stimulation. In vitro multistep ovarian tissue static culture followed by mature oocyte generation, fertilization, and embryo transfer promises to use the reserve of dormant follicles. Unfortunately, static in vitro culture of ovarian tissue does not promote development of primordial to secondary follicles or sustain follicle viability and thereby limits the number of obtainable mature oocytes. Enhancing oxygen transport to and exerting mechanical stimulation on ovarian tissue in a dynamic bioreactor may more closely mimic the physiological microenvironment and thus promote follicle activation, development, and viability.Materials and Methods: The most transport-effective dynamic bioreactor design was modified using 3D models of medium and oxygen transport to maximize strip perifusion and apply tissue fluid dynamic shear stresses and direct compressive strains to elicit tissue response. Prototypes of the final bioreactor design were manufactured with materials of varying cytocompatibility and assessed by testing the effect of leachables on sperm motility. Effectiveness of the bioreactor culture was characterized against static controls by culturing fresh bovine ovarian tissue strips for 7 days at 4.8 × 10−5 m/s medium filtration flux in air at −15% maximal total compressive strain and by assessing follicle development, health, and viability.Results and Conclusions: Culture in dynamic bioreactors promoted effective oxygen transport to tissues and stimulated tissues with strains and fluid dynamic shear stresses that, although non-uniform, significantly influenced tissue metabolism. Tissue strip culture in bioreactors made of cytocompatible polypropylene preserved follicle viability and promoted follicle development better than static culture, less so in bioreactors made of cytotoxic ABS-like resin.
Rotation-traction manipulation induced intradiskal pressure changes in cervical spine—an in vitro study
Authors: Han Changxiao , Feng Minshan , Wen Haibao , Yin Xunlu , Li Jing , Du Wuyin , Peng Bochen , Liu Guangwei , Zhu Liguo 
Published: February 2024

Objective: Evaluate the effect of rotation-traction manipulation on intradiskal pressure in human cervical spine specimen with different force and duration parameters, and compare the intradiskal pressure changes between rotation-traction manipulation and traction.Methods: Seven human cervical spine specimens were included in this study. The intradiskal pressure was measured by miniature pressure sensor implanting in the nucleus pulposus. rotation-traction manipulation and cervical spine traction were simulated using the MTS biomechanical machine. Varied thrust forces (50N, 150N, and 250N) and durations (0.05 s, 0.1 s, and 0.15 s) were applied during rotation-traction manipulation with Intradiscal pressure recorded in the neutral position, rotation-anteflexion position, preloading, and thrusting phases. Futuremore, we documented changes in intradiscal pressure during cervical spine traction with different loading forces (50N, 150N, and 250N). And a comparative analysis was performed to discern the impact on intradiscal pressure between manipulation and traction.Results: Manipulation application induced a significant reduction in intradiscal pressure during preloading and thrusting phases for each cervical intervertebral disc (p &lt; 0.05). When adjusting thrust parameters, a discernible decrease in intradiscal pressure was observed with increasing thrust force, and the variations between different thrust forces were statistically significant (p &lt; 0.05). Conversely, changes in duration did not yield a significant impact on intradiscal pressure (p &gt; 0.05). Additionally, after traction with varying loading forces (50N, 150N, 250N), a noteworthy decrease in intradiscal pressure was observed (p &lt; 0.05). And a comparative analysis revealed that rotation-traction manipulation more markedly reduced intradiscal pressure compared to traction alone (p &lt; 0.05).Conclusion: Both rotation-traction manipulation and cervical spine traction can reduce intradiscal pressure, exhibiting a positive correlation with force. Notably, manipulation elicits more pronounced and immediate decompression effect, contributing a potential biomechanical rationale for its therapeutic efficacy.
Colorimetric sensing of hydrogen peroxide using capped Morus nigra-sawdust deposited zinc oxide nanoparticles via Trigonella foenum extract
Authors: Nishan Umar , Zahra Tabassum , Badshah Amir , Muhammad Nawshad , Afridi Saifullah , Shah Mohibullah , Khan Naeem , Asad Muhammad , Ullah Riaz , Ali Essam A. , Chen Ke 
Published: February 2024

Hydrogen peroxide (H2O2) is one of the main byproducts of most enzymatic reactions, and its detection is very important in disease conditions. Due to its essential role in healthcare, the food industry, and environmental research, accurate H2O2 determination is a prerequisite. In the present work, Morus nigra sawdust deposited zinc oxide (ZnO) nanoparticles (NPs) were synthesized by the use of Trigonella foenum extract via a hydrothermal process. The synthesized platform was characterized by various techniques, including UV-Vis, FTIR, XRD, SEM, EDX, etc. FTIR confirmed the presence of a Zn‒O characteristic peak, and XRD showed the hexagonal phase of ZnO NPs with a 35 nm particle size. The EDX analysis confirmed the presence of Zn and O. SEM images showed that the as-prepared nanoparticles are distributed uniformly on the surface of sawdust. The proposed platform (acetic acid-capped ZnO NPs deposited sawdust) functions as a mimic enzyme for the detection of H2O2 in the presence of 3,3′,5,5′-tetramethylbenzidine (TMB) colorimetrically. To get the best results, many key parameters, such as the amount of sawdust-deposited nanoparticles, TMB concentration, pH, and incubation time were optimized. With a linear range of 0.001–0.360 μM and an R2 value of 0.999, the proposed biosensor’s 0.81 nM limit of quantification (LOQ) and 0.24 nM limit of detection (LOD) were predicted, respectively. The best response for the proposed biosensor was observed at pH 7, room temperature, and 5 min of incubation time. The acetic acid-capped sawdust deposited ZnO NPs biosensor was also used to detect H2O2 in blood serum samples of diabetic patients and suggest a suitable candidate for in vitro diagnostics and commercial purposes.
Innovative Sol-gel functionalized polyurethane foam for sustainable water purification and analytical advances
Authors: Alwael H. , Alsulami A. N. , Abduljabbar T. N. , Oubaha M. , El-Shahawi M. S. 
Published: February 2024

Nanomaterial combined polymeric membranes such as polyurethane foams (PUFs) have garnered enormous attention in the field of water purification due to their ease of management and surface modification, cost-effectiveness, and mechanical, chemical, and thermal properties. Thus, this study reports the use of novel Sol-gel impregnated polyurethane foams (Sol-gel/PUFs) as new dispersive solid phase microextractors (d- µ SPME) for the efficient separation and subsequent spectrophotometric detection of Eosin Y (EY) textile dye in an aqueous solution with a pH of 3–3.8. The Sol gel, PUFs, and Sol gel–impregnated PUFs were characterized using scanning electron microscopy (SEM), goniometry measurements, dynamic light scattering (DLS), energy dispersive spectroscopy (EDS), UV-Visible, and FTIR spectra. Batch experiment results displayed a remarkable removal percentage (96% ± 5.4%) of the EY from the aqueous solution, with the total sorption time not exceeding 60 min. These data indicate rate-limited sorption via diffusion and/or surface complex ion associate formations after the rapid initial sorption steps. A pseudo-second order kinetic model thoroughly explained the sorption kinetics, providing a sorption capacity (qe) of 37.64 mg g−1, a half-life time (t1/2) of 0.8 ± 0.01 min, and intrinsic penetration control dye retention. The thermodynamic results revealed a negative value for ΔG⁰ (−78.07 kJ mol−1 at 293 K), clearly signifying that the dye uptake was spontaneous, as well as a negative value for ΔH⁰ (−69.58 kJ mol−1) and a positive value for ΔS⁰ (147.65 J mol−1 K−1), making clear the exothermic nature of EY adsorption onto the sorbent, with a growth in randomness at the molecular level. A ternary retention mechanism is proposed, involving the “weak base anion exchanger” of {(–CH2–OH+ –CH2–) (Dye anion)−}Sol-gel/PUF and/or {(–NH2+ –COO-) (Dye anion)−}Sol-gel/PUF via solvent extraction and “surface adsorption” of the dye anion on/in the Sol-gel/PUFs membranes in addition to H-bonding, including surface complexation and electrostatic π-π interaction, between the dye and the silicon/zirconium oxide (Si-O-Zr) and siloxane (Si-O-Si) groups on the sorbent. Complete extraction and recovery (93.65 ± 0.2, −102.28 ± 2.01) of EY dye with NaOH (0.5 M) as a proper eluting agent was achieved using a sorbent-packed mini column. In addition, the established extractor displayed excellent reusability and does not require organic solvents for EY enrichment in water samples, making it a talented nominee as a novel sorbent for EY sorption from wastewater. This study is of great consequence for expanding the applicatio1n of Sol-gel/PUFs in developing innovative spectrophotometric sensing strategies for dye determination. In view of this, it would also be remarkable to perform future studies to explore the analytical implications of this extractor regarding safety and environmental and public health issues associated to the pollutant.
The TRPM2 ion channel regulates metabolic and thermogenic adaptations in adipose tissue of cold-exposed mice
Authors: Benzi Andrea , Heine Markus , Spinelli Sonia , Salis Annalisa , Worthmann Anna , Diercks Björn , Astigiano Cecilia , Pérez Mato Raúl , Memushaj Adela , Sturla Laura , Vellone Valerio , Damonte Gianluca , Jaeckstein Michelle Y. , Koch-Nolte Friedrich , Mittrücker Hans-Willi , Guse Andreas H. , De Flora Antonio , Heeren Joerg , Bruzzone Santina 
Published: February 2024

IntroductionDuring thermogenesis, adipose tissue (AT) becomes more active and enhances oxidative metabolism. The promotion of this process in white AT (WAT) is called “browning” and, together with the brown AT (BAT) activation, is considered as a promising approach to counteract obesity and metabolic diseases. Transient receptor potential cation channel, subfamily M, member 2 (TRPM2), is an ion channel that allows extracellular Ca2+ influx into the cytosol, and is gated by adenosine diphosphate ribose (ADPR), produced from NAD+ degradation. The aim of this study was to investigate the relevance of TRPM2 in the regulation of energy metabolism in BAT, WAT, and liver during thermogenesis.MethodsWild type (WT) and Trpm2-/- mice were exposed to 6°C and BAT, WAT and liver were collected to evaluate mRNA, protein levels and ADPR content. Furthermore, O2 consumption, CO2 production and energy expenditure were measured in these mice upon thermogenic stimulation. Finally, the effect of the pharmacological inhibition of TRPM2 was assessed in primary adipocytes, evaluating the response upon stimulation with the β-adrenergic receptor agonist CL316,243.ResultsTrpm2-/- mice displayed lower expression of browning markers in AT and lower energy expenditure in response to thermogenic stimulus, compared to WT animals. Trpm2 gene overexpression was observed in WAT, BAT and liver upon cold exposure. In addition, ADPR levels and mono/poly-ADPR hydrolases expression were higher in mice exposed to cold, compared to control mice, likely mediating ADPR generation.DiscussionOur data indicate TRPM2 as a fundamental player in BAT activation and WAT browning. TRPM2 agonists may represent new pharmacological strategies to fight obesity.
Effect of traditional Chinese fitness exercises on bone mineral density in postmenopausal women: a network meta-analysis of randomized controlled trials
Authors: Liu Shijie , Wu Sijun , Qi Juancai , Wang Lin 
Published: February 2024

We aimed to evaluate the clinical efficacy of five traditional Chinese fitness exercises (Baduanjin, Taijiquan, Wuqinxi, Yijinjing, and Liuzijue), as well as their efficacy when combined with drug therapy, in the treatment of decreased bone mineral density in postmenopausal womenMethodsThis study strictly followed the evaluation guidelines of PRISMA and followed the “PICOS” principle outlined in the Cochrane Handbook. We performed a systematic search on Web of Science, Springer Link, Scopus, EMBASE, EBSCO, PubMed, the Cochrane Library, CNKI, Wanfang, CBMdisc, and the VIP Database, and we targeted RCTs studying the effect of TCE on BMD in postmenopausal women published prior to September 2023. The quality of the literature and the risk of bias of the included studies were assessed according to ROB2 and GRADE criteria, and data analysis was performed using Stata 14.ResultsA total of 33 RCTs (3658 post-menopausal women) were included. Network meta-analysis showed that Taiji (SMD=0.72, 95% CI: 0.22, 1.21, P&lt;0.01) and Yijinjing (SMD=0.51, 95% CI: 0.03, 0.99, P&lt;0.05) were significantly superior to conventional rehabilitation in lumbar BMD. In terms of improvement of femoral neck BMD, Baduanjin (SMD=1.63, 95% CI: -3.58, 6.85, P&lt;0.001) and Taiji (SMD=0.46, 95% CI: 0.14, 0.79, P&lt;0.05) had statistically different outcomes to conventional rehabilitation. Regarding Ward’s triangle BMD, Taiji (SMD= 0.32, 95% CI: 0.14, 0.50, P&lt; 0.05) had statistically different outcomes to conventional rehabilitation. The results of the SUCRA probability ranking showed that Baduanjin + drug interventions achieved the most significant improvement in lumbar BMD (SUCRA=83.6%) and femoral neck BMD (SUCRA=90.2%). Taiji + drug interventions most effectively improved Ward’s triangle BMD (SUCRA=86.0%). In terms of traditional Chinese fitness exercises alone, Taiji was the most effective in improving lumbar BMD (SUCRA=64.4%) and Ward’s triangle BMD (SUCRA=46.8%), and Baduanjin was the most effective in treating femoral neck BMD (SUCRA=89.9%).ConclusionTraditional Chinese fitness exercises can significantly improve the BMD levels of postmenopausal women. Taiji, Yijinjing, and Baduanjin combined with medication showed better intervention effects overall. However, due to the limitations of the number of studies and sample sizes of individual interventions, definitive conclusions need to be verified by more high-quality studies.
Glycemic control in children with type 1 diabetes treated with the advanced hybrid closed loop system 2-year prospective, observational, two-center study
Authors: Seget Sebastian , Chobot Agata , Tarasiewicz Mateusz , Bielawska Anna , Rusak Ewa , Ochab Agnieszka , Polanska Joanna , Jarosz-Chobot Przemysława 
Published: February 2024

Background and aimsMiniMed 780G is the first Advanced Hybrid Closed Loop (AHCL) system in Poland, approved in the EU in 2020. To date, observations of glycemic control up to 12 months have been published. This study aimed to analyze glycemic control and anthropometric parameters in children and adolescents with type 1 diabetes (T1D) after two years of using the AHCL system.Materials and methodsWe prospectively collected anthropometric data, pump, and continuous glucose records of fifty T1D children (9.9 ± 2.4 years, 24 (48%) boys, T1D for 3.9 ± 2.56 years) using an AHCL system. We compared the two-week AHCL records obtained after AHCL enrollment with data 6, 12, and 24 months after starting AHCL.ResultsTime in range (70-180 mg/dl) and BMI z-score did not change during the 2 years of observation (p&gt;0.05). The percentage of autocorrection in total daily insulin increased significantly (p&lt;0.005).ConclusionGlycemic control in the investigated group of children with T1D treated with the AHCL system for 2 years remained stable. Children in this group maintained weight and optimal metabolic control, most likely due to autocorrection boluses.