bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping
Authors: Dong Jia , Croslow Seth W , Lane Stephan T , Castro Daniel C , Blanford Jantana , Zhou Shuaizhen , Park Kiyoul , Burgess Steven , Root Mike , Cahoon Edgar B , Shanklin John , Sweedler Jonathan V , Zhao Huimin , Hudson Matthew E 
Published: February 2025

Abstract
Plant bioengineering is a time-consuming and labor-intensive process with no guarantee of achieving desired traits. Here, we present a fast, automated, scalable, high-throughput pipeline for plant bioengineering (FAST-PB) in maize (Zea mays) and Nicotiana benthamiana. FAST-PB enables genome editing and product characterization by integrating automated biofoundry engineering of callus and protoplast cells with single-cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We first demonstrated that FAST-PB could streamline Golden Gate cloning, with the capacity to construct 96 vectors in parallel. Using FAST-PB in protoplasts, we found that PEG2050 increased transfection efficiency by over 45%. For proof-of-concept, we established a reporter-gene-free method for CRISPR editing and phenotyping via mutation of high chlorophyll fluorescence 136. We show that diverse lipids were enhanced up to 6-fold using CRISPR activation of lipid controlling genes. In callus cells, an automated transformation platform was employed to regenerate plants with enhanced lipid traits through introducing multigene cassettes. Lastly, FAST-PB enabled high-throughput single-cell lipid profiling by integrating MALDI-MS with the biofoundry, protoplast, and callus cells, differentiating engineered and unengineered cells using single-cell lipidomics. These innovations massively increase the throughput of synthetic biology, genome editing, and metabolic engineering and change what is possible using single-cell metabolomics in plants.
Oxidative adaptations in prokaryotes imply the oxygenic photosynthesis before crown-group Cyanobacteria
Authors: Zeng Zichao , Li Liuyang , Wang Heng , Tao Yuxin , Lv Zhenbo , Wang Fengping , Wang Yinzhao 
Published: February 2025

Abstract
The metabolic transition from anaerobic to aerobic in prokaryotes reflects adaptations to oxidative stress. Methanogen, one of the earliest life forms on Earth, has evolved into three major groups within the Euryarchaeota, exhibiting different phylogenetic affiliations and metabolic characters. In comparison with other strictly anaerobic methanogenic groups, the Class II methanogens possess a better capability to adapt to limited oxygen pressure. Cyanobacteria is considered the first and only prokaryote evolving oxygenic photosynthesis and is responsible for the Great Oxidation Event on Earth. However, the connection between oxygenic Cyanobacteria and evolutionary adaptations to oxidative stress in prokaryotes remains elusive. Here, through the gene encoding structural maintenance of chromosomes (SMC) protein, which was horizontally transferred from ancient Class II methanogens to the last common ancestor of the crown-group Cyanobacteria, we demonstrate that the origin of extant Cyanobacteria was undoubtedly posterior to the occurrence of oxygen-tolerant Class II methanogens. In addition, we found that certain prokaryotic lineages had evolved the tolerance mechanisms against oxidative stress before the origin of extant Cyanobacteria. The contradiction that oxidative adaptations in Class II methanogens and other prokaryotes predating the crown-group oxygenic Cyanobacteria implies the existence of more ancient biological oxygenesis. We propose that these potential oxygenic organisms might represent the extinct phototrophs and first emerge during the Paleoarchean, contributing to the oxidative adaptations in the prokaryotic tree of life and facilitating the dispersal of reaction centers across the bacterial domain.
Diagnosis of Alcohol Use Disorder and Deaths Related to Alcohol, Drug Overdose, or Suicide among Post-9/11 Active Duty Service Members and Veterans Following Traumatic Brain Injury
Authors: Song Kangwon , Amuan Megan E. , Adams Rachel Sayko , Kennedy Eamonn , Gordon Adam J. , Carlson Kathleen F. , Pogoda Terri K. , Meyer Eric G. , Cochran Jerry , Spevak Christopher , Pugh Mary Jo 
Published: February 2025

Objectives
The association between traumatic brain injury (TBI) and alcohol use disorder (AUD) is known, but the extent of TBI’s role in developing AUD remains unclear. This study examines the association between TBI severity with subsequent AUD diagnosis, and hazard for death due to alcohol, drug overdose, or suicide.

Methods
Data from a national US military/veteran cohort (October 1999–September 2016, followed until September 2020) were analyzed using Fine-Gray competing risk models to investigate the relationships between TBI exposure, subsequent AUD, and hazards of death due to specific causes (alcohol, drug overdose, or suicide).

Results
TBI severity correlated with an increased likelihood of an incident AUD diagnosis: mild TBI (hazard ratio [HR]: 1.25, 95% confidence interval [CI] 1.22–1.27), moderate-severe TBI (HR: 1.34, 95% CI 1.32–1.37), and penetrating TBI (HR: 1.90, 95% CI 1.86–1.94). For those who developed AUD, TBI was associated with a higher hazard of death from specific causes such as alcohol, drug overdose, or suicide (HR: 2.47 (95% CI 2.03–3.02) for mild TBI, 4.25 (95% CI 3.49–5.17) for moderate-severe TBI, and 3.39 (95% CI 2.80–4.13) for penetrating TBI.

Conclusions
Veterans with TBI were more likely to develop AUD and experience increased mortality, even after adjusting for demographic and clinical factors. Care strategies that are sensitive to the cognitive and/or emotional impairments associated with varying levels of TBI may lead to better outcomes, reducing both AUD and mortality rates. Further research is needed to develop evidence-based methods for integrating TBI and AUD care.
Midterm Outcomes of Shoulder Dislocations in Brachial Plexus Birth Injuries Treated With Injection Botulinum Toxin A
Authors: Shah Maulin M. , Mammen Joyance J. , Jain Meet , Ponugoti Godhasiri , Naik Nischal , Patel Tejas , Rubaiath Tashfin , Vijay Preksha 
Published: February 2025

Background:
Shoulder dislocation can be seen early in infancy and can be diagnosed by shoulder ultrasound. Botulinum toxin injection in the internal rotators of the shoulder can be used to as an adjunct to relieve the internal rotation contracture. The aim of this study was to evaluate the intermediate-term outcomes of shoulder dislocations treated with injection botulinum toxin and to identify the factors determining the outcomes of the procedure.

Methods:
Eighty-six children with ultrasound diagnosed shoulder dislocation underwent injection BTX-A between 2011 and 2022. Children who underwent nerve surgeries and the ones with inadequate follow-up were excluded. Sixty-two children were followed up for a mean duration of 36 months. Injection botulinum toxin A 2 U/kg body weight was injected each into the subscapularis and the pectoralis major. After manual stretching of contracted anterior soft tissue and shoulder capsule, a shoulder spica was applied for 3 weeks. Hospital for Sick Children Active Movement Scale (HSC-AMS) score and active and passive shoulder was recorded before and after the procedure and the requirement for further interventions was noted.

Results:
Outcomes were divided into 3 categories: group A—27 (44%) children who did not require any further surgery in our follow-up period; group B—27 (44%) needed further procedure for recurrence of IR contracture; and group C—8 (12%) developed external rotation contracture later. Children in group A had statistically significant higher preinjection HSC-AMS score for elbow flexion and shoulder abduction than the other groups. Children in group C had greater passive external rotation and weak elbow extension and wrist dorsiflexion as compared with the other 2 groups.

Conclusions:
Injection botulinum toxin A is effective in treating shoulder dislocations in children with early recruitment of C5 to 6 muscles, while children with involvement of C7 muscles may develop an external rotation contracture subsequently, hence this procedure should be avoided.

Level of evidence:
Level IV—therapeutic.
Clinical Utility of CRISPR-Based RID-MyC Assay in Smear and Culture-Negative Fungal Keratitis: A Case Series
Authors: Deivarajan Hanith Raj , Chelliah Dharani , Sethupathy Ramkumar Prabhakara , Nandhakumar Dharsini , Chacko Bennet Mathew , Raghavan Anita , Pandian Jaishree , Prajna Lalitha , Prajna Venkatesh N. , Narendran Siddharth 
Published: February 2025

Purpose:
To assess the clinical utility of the clustered regularly interspaced short palindromic repeats/Cas12a-based RID-MyC assay in diagnosing Fungal Keratitis (FK) in cases where conventional smear and culture methods fail to identify the causative pathogen.

Methods:
This retrospective case series included 5 patients with clinically suspected FK and negative smear and culture results who were evaluated in the Cornea Department at Aravind Eye Hospital, Coimbatore, India, between January 1, 2024, and March 31, 2024. The primary outcome was the diagnostic performance of the RID-MyC assay in detecting fungal nucleic acids in cases of suspected FK with negative smear and culture results. In vivo confocal microscopy served as a reference standard to validate the RID-MyC assay findings.

Results:
The RID-MyC assay successfully detected fungal nucleic acids in 3 cases, corroborated by in vivo confocal microscopy findings suggestive of fungal filaments, leading to targeted antifungal therapy and resolution of the infections. In addition, 2 cases tested negative for fungal nucleic acids, aligning with clinical and confocal evidence of nonfungal etiology, thus guiding appropriate alternative treatments that led to clinical improvement.

Conclusions:
The RID-MyC assay demonstrates clinical utility in diagnosing FK in scenarios where conventional smear and culture methods prove inadequate, such as in cases with prior antifungal therapy or polymicrobial infections. This assay facilitates accurate diagnosis and timely initiation of appropriate antifungal treatment without the need for sophisticated equipment or expertise, making it particularly valuable in resource-limited settings.
Differential Pattern of Symptom Correlation With Acute Respiratory Infections in Korea
Authors: Kim Jinsoo , Jung Gyoohwan , Kim Soyeoun 
Published: February 2025

Background:
Acute respiratory infections (ARIs) pose global health challenges, with major outbreaks affecting healthcare systems and resulting in significant morbidity and mortality. We aimed to identify distinctive signs or symptoms correlated with ARIs for utilizing syndromic surveillance.

Methods:
We used data from national Korean databases to examine correlations between various symptoms and the reported ARI viruses in children aged under and over 5 years.

Results:
In children under 5 years old, respiratory symptoms were strongly correlated with human adenovirus, human respiratory syncytial virus, and human rhinovirus. Patients aged over 5 years displayed more diverse patterns, with varied correlations. The cases of fever were a strong indicator of respiratory viruses (human adenovirus, human parainfluenza viruses, and human rhinovirus) in children under 5 years old, while those over 5 years showed symptoms such as smell and taste disturbances.

Conclusions:
These findings emphasize the correlation between various symptoms and ARIs across different age groups and may help to improve syndromic surveillance systems.
The Impact of Piano Game-Based Learning on the Mental Health of Elderly Individuals
Authors: Song Jie , Guan Kean 
Published: February 2025

Abstract
The main research purpose was to investigate the impact of piano courses on the mental health of the elderly. The sample consisted of 30 individuals. The age range of the participants was from 60 to 70 years. For 6 months, the respondents had piano lessons two times a week. One lesson lasted 2 hours and included the development of playing skills and improvisation techniques. The results confirmed the validity of the proposed learning strategy. The Taylor anxiety scale decreased by 19 points. These results showed a decrease of more than half of its original value. The Beck test for depression decreased by 21.5 points. The result shows a decrease two times. The test on the emotional background (the WAM questionnaire) shows an improvement in Well-Being by 3.3 points. In the group of respondents, Activity increased by 3.3 points, and the Mood factor increased by 3.5 points. The direction of further research should be related to the study of the impact of musical practices on various components of the clinical picture of depression and negative emotional states. The results can find practical implementation in the practice of psychotherapeutic work in reducing anxiety and eliminating depression in the elderly in clinics, hospitals, and other medical institutions.
Candidate transmission survival genome ofMycobacterium tuberculosis
Authors: Mishra Saurabh , Singh Prabhat Ranjan , Hu Xiaoyi , Lopez-Quezada Landys , Jinich Adrian , Jahn Robin , Geurts Luc , Shen Naijian , DeJesus Michael A. , Hartman Travis , Rhee Kyu , Zimmerman Matthew , Dartois Veronique , Jones Richard M. , Jiang Xiuju , Almada-Monter Ricardo , Bourouiba Lydia , Nathan Carl 
Published: February 2025

AbstractMycobacterium tuberculosis(Mtb), a leading cause of death from infection, completes its life cycle entirely in humans except for transmission through the air. To begin to understand how Mtb survives aerosolization, we mimicked liquid and atmospheric conditions experienced by Mtb before and after exhalation using a model aerosol fluid (MAF) based on the water-soluble, lipidic and cellular constituents of necrotic tuberculosis lesions. MAF induced drug tolerance in Mtb, remodeled its transcriptome and protected Mtb from dying in microdroplets desiccating in air. Yet survival was not passive: Mtb appeared to rely on hundreds of genes to survive conditions associated with transmission. Essential genes subserving proteostasis offered most protection. A large number of conventionally nonessential genes appeared to contribute as well, including genes encoding proteins that resemble anti-desiccants. The candidate transmission survival genome of Mtb may offer opportunities to reduce transmission of tuberculosis.Significance StatementMycobacterium tuberculosis(Mtb) travels from the lungs of one person through the air to the lungs of another and survives multiple stresses en route, including changes in temperature and in concentrations of oxygen, carbon dioxide, hydrogen ions, salts and organic solutes. Here we present a genetically tractable model of transmission to begin the identification of the transmission survival genome of Mtb. We devised a fluid that mimics TB lesions, found that it protects Mtb from transmission-related stresses, associated this with the structure of the droplets as they dry and their ability to retain water, and used it to query the potential contribution of each of Mtb’s genes to Mtb’s survival in models of three sequential stages of transmission.
Identification and Characterization of Interacting Proteins of TARANI/Ubiquitin Specific Protease-14 inArabidopsis thaliana
Authors: Hegde Anjana S , Nath Utpal 
Published: February 2025

AbstractUbiquitin proteases play a crucial role in protein degradation and turnover by regulating the cleavage of polyubiquitin chains. TARANI/UBIQUITIN SPECIFIC PROTEASE-14 (TNI/UBP14) specifically cleaves Lys-48-linked and linear polyubiquitin chains into mono-ubiquitins. Thetnimutant exhibits pleiotropic phenotypes, including cup-shaped leaves, tri-cotyledons, reduced lateral roots, and increased petal number, though the underlying mechanisms driving these phenotypes remain unclear. In this study, we generated TNI transgenic lines and employed immunoprecipitation mass spectrometry, proximity labelling, and yeast two-hybrid screening to identify TNI’s interacting proteins. These analyses revealed 92 interactors involved in diverse biological processes, including protein and carbohydrate metabolism, light signalling, and intracellular transport. Subcellular localization analysis showed that many of the interacting proteins are located in the nucleus and cytoplasm, suggesting that TNI’s nuclear localization may regulate gene function. We further validated thein plantabiological significance of ULTRAPETALA 2 and HASPIN KINASE as key interacting partners of TNI. These findings uncover previously uncharacterized functions of TNI/UBP14, shedding light on its central role in cellular processes and providing insights into its regulatory mechanisms—an area that has remained largely unexplored until now.Summary statementThe proteins that interact with the TARANI/ Ubiquitin protease 14 in vivo have been identified using immunoprecipitation mass-spectrometry methods. Identification of non-overlapping targets highlight the importance of using diverse protein identification methods.
Enhancing loyalty in luxury fashion with AI: the mediation role of customer relationships
Authors: Khamoushi Sahne Seyed Sina , Kalantari Daronkola Hassan 
Published: February 2025

PurposeThis study aims to investigate the impact of artificial intelligence (AI) on customer loyalty in the luxury fashion market. It explores how AI-driven tools influence customer trust, satisfaction, commitment and engagement, which in turn affect loyalty. By examining these relationships, the study provides insights into the acceptance and effectiveness of AI technologies in enhancing customer loyalty within the luxury fashion sector.Design/methodology/approachThis study employs structural equation modelling (SEM) to analyse data collected from 406 luxury consumers in Iran. The data was gathered using a targeted sampling procedure, leveraging DigiKala’s e-commerce platform. A comprehensive literature review informed the measurement items, and a seven-point Likert scale was used. The methodology includes confirmatory factor analysis (CFA) to assess the reliability and validity of the constructs, followed by hypothesis testing through SEM.FindingsThe study reveals that AI significantly enhances customer loyalty in the luxury fashion market by positively influencing trust, satisfaction, commitment and engagement. Satisfaction and engagement were found to be key mediators between AI and loyalty, while trust had no direct impact on loyalty. The results underscore the importance of AI-driven personalized experiences in fostering stronger customer relationships and loyalty.Originality/valueThis study is one of the first to explore the impact of AI on customer loyalty in the luxury fashion market, using a comprehensive model that includes trust, satisfaction, commitment and engagement as mediators. It extends the stimulus-organism-response (SOR) and technology acceptance model (TAM) frameworks, offering valuable insights for luxury brands on how AI can be leveraged to enhance customer relationships and loyalty.
Resilience capabilities and performance dimensions of manufacturing supply chains in a developing economy
Authors: Opoku Richard Kofi 
Published: February 2025

PurposeThis paper investigates whether resilience capabilities influence manufacturing performance dimensions. Specifically, it empirically analyses how supply chain agility, alertness, adaptability and preparedness affect manufacturing firms’ operational and sustainable (economic, social and environmental) performance aspects.Design/methodology/approachThe paper employed a deductive approach and an explanatory design. It gathered survey data from 285 managers in 5,329 Ghanaian manufacturing firms and analysed it using structural equation modelling.FindingsThe study found resilience capabilities comprising agility, alertness and adaptability to significantly and positively predict changes in manufacturing firms’ sustainable (environmental, economic and social) and operational performance. However, the preparedness capability positively impacts the firms’ operational and environmental performance, not economic and social.Research limitations/implicationsThis paper is restricted to Ghana’s manufacturing industry. Underpinned by the dynamic capabilities theory and extensive empirical reviews, the model was developed with four resilient capabilities and four manufacturing performance dimensions.Practical implicationsThe study highlights the relevance of resilience in today’s highly disruptive manufacturing environment for achieving sustainable and operational performance. It encourages manufacturing firms to prioritise heavy investments in alertness, adaptability and agile capabilities to overcome supply chain disruptions and enhance sustainable and operational excellence. It also offers significant insights for policymakers, managers and industry players to advance resilience capabilities and swiftly detect and recover from emerging disturbances in manufacturing supply chains, leading to higher performance.Social implicationsThe study contributes to resource conservation and a more sustainable future by projecting resilient capabilities in today’s disruptive environments. The shift towards SCR can influence public attitudes and opinions toward manufacturing and contribute to firms’ sustainability goals.Originality/valueThis study is the first to investigate the linkages between resilient capabilities and performance aspects simultaneously in less developed economies like Ghana. In these economies, manufacturing supply chains often face varying risks that continue to disrupt their operations and sustainability goals.
Revolutionizing HRM: a review of human-robot collaboration in HRM functions and the imperative of change readiness
Authors: Shahid Sehrish , Kaur Kuldeep , Mohyuddin Syed Mofazzal , Prikshat Verma , Patel Parth 
Published: February 2025

PurposeThe purpose of the paper is to conduct a review of the literature on human-robot collaboration across different functions and activities of human resource management (HRM) and discuss its importance for change readiness in organizations.Design/methodology/approachA bibliometric analysis was conducted to identify emerging research themes in the fields of human resources (HR) and robotics, including change readiness. Based on the initial results of the bibliometric analysis, a systematic literature review was subsequently performed to gain a more specific understanding of research across various HRM functions and change readiness.FindingsThe results from bibliometric analysis and systematic review highlight that technological progression in HRM, such as AI-driven staffing and training techniques, improves effectiveness and personalization but raises concerns about privacy and job scrutiny. AI and robotics in performance evaluation enhance objectivity and reduce subjectivity, which can lead to disengagement. Generational differences, cultural factors and emotional quotient complicate readiness to adopt new technologies. The research emphasizes balancing technological effectiveness with employee involvement and meaningfulness to ensure successful implementation and engagement.Originality/valueThis paper synthesizes existing research, including literature, theoretical concepts and models, to identify best practices and successful strategies for implementing human-robot collaboration in HRM functions. It highlights gaps in the current literature and suggests areas for future research to advance the field of human-robot collaboration in HRM. By doing so, this paper enhances theoretical understanding while offering practical insights essential for effective change management.