bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transfer of cetirizine/levocetirizine into human breast milk and estimation of drug exposure to infants through breastfeeding: A human lactation study from the ConcePTION project
Authors: Nordeng Hedvig , Wegler Christine , Lindqvist Annika , Melander Erik , Magnusson Mikaela , Gandia Peggy , Panchaud Alice , Baranczewski Pawel , Spigset Olav 
Published: October 2023

AbstractData on drug transfer into human breast milk are sparse. This study aimed to quantify concentrations of cetirizine and levocetirizine in breast milk and to estimate drug exposure to infants. Breastfeeding women at least 8 weeks postpartum and using cetirizine or its pure (R)‐enantiomer levocetirizine were eligible to participate. Breast milk samples were collected at six predefined times during a dose interval (0, 2, 4, 8, 12 and 24 h after drug intake) at steady state. Infant drug exposure was estimated by calculating the absolute infant dose (AID) and the weight‐adjusted relative infant dose (RID). In total, 32 women were eligible for final inclusion, 31 women using cetirizine and one woman using levocetirizine. Means of the individual maximum and average cetirizine milk concentrations were 41.0 and 16.8 μg/L, respectively. Maximum concentrations occurred on average 2.4 h after intake, and the mean half‐life in milk was 7.0 h. Estimated AID and RID for cetirizine in a day were 2.5 μg/kg and 1.9%, respectively. The corresponding values for levocetirizine were 1.1 μg/kg and 1.9%. No severe adverse events were reported. Our findings demonstrate that the transfer of cetirizine and levocetirizine into breast milk is low and compatible with breastfeeding.
Evaluating hybrid speciation and swamping in wild carnivores with a decision‐tree approach
Authors: Tensen Laura , Fischer Klaus 
Published: October 2023

AbstractHybridization is an important evolutionary force with a principal role in the origin of new species, known as hybrid speciation. However, ongoing hybridization can create hybrid swamping, in which parental genomes are completely lost. This can become a biodiversity threat if it involves species that have adapted to certain environmental conditions and occur nowhere else. Because conservation scientists commonly have a negative attitude toward hybrids, it is important to improve understanding of the influence of interspecific gene flow on the persistence of species. We reviewed the literature on species hybridization to build a list of all known cases in the order Carnivora. To examine the relative impact, we also noted level of introgression, whether fertile offspring were produced, and whether there was mention of negative or positive evolutionary effects (hybrid speciation and swamping). To evaluate the conservation implications of hybrids, we developed a decision‐making tree with which to determine which actions should be taken to manage hybrid species. We found 53 hybrids involving 68 unique taxa, which is roughly 23% of all carnivore species. They mainly involved monophyletic (83%) and sympatric species (75%). For 2 species, the outcome of the assessment was to eliminate or restrict the hybrids: Ethiopian wolf (Canis simensis) and Scottish wildcat (Felis silvestris silvestris). Both species hybridize with their domestic conspecifics. For all other cases, we suggest hybrids be protected in the same manner as native species. We found no evidence of genomic extinction in Carnivora. To the contrary, some species appear to be of hybrid origin, such as the Asiatic black bear (Ursus thibetanus) and African golden wolf (Canis lupaster). Other positive outcomes of hybridization are novel genetic diversity, adaptation to extreme environments, and increased reproductive fitness. These outcomes are particularly valuable for counterbalancing genetic drift and enabling adaptive introgression in a human‐dominated world.
Effects of captions on viewers’ perceptions of images depicting human−primate interaction
Authors: Freund Cathryn A. , Cronin Katherine A. , Huang Michelle , Robinson Natalie J. , Yoo Boaz , DiGiorgio Andrea L. 
Published: October 2023

AbstractPromoting human contact with wildlife can be harmful to animal conservation and welfare by exposing animals to unsafe situations and driving demand for wildlife tourism and exotic pets. Conservationists and researchers justifiably use social media to raise awareness, but professionals posting pictures of themselves with animals can have unintended negative consequences for conservation. Though the International Union for Conservation of Nature Best Practice Guidelines for Responsible Images of Non‐Human Primates suggests researchers and animal professionals provide context in captions of images of humans interacting with primates, there is little research investigating whether this approach is effective. We investigated whether informative captions affect viewers’ desires to have primates as pets and attitudes toward wildlife conservation. Using 4 mock Instagram posts depicting human−gorilla and human−slender loris interactions, we surveyed 2977 respondents to assess the effect of captions on viewers’ perceptions of the images and primate conservation. Likert scale response data were analyzed with ordered probit regression models. Captions clearly contextualizing an image as research resulted in a significantly higher agreement that posts depicted wildlife research (gorilla β = 0.28 [SE 0.06], p < 0.001; loris β = 0.18 [0.06], p = 0.002), but such captions resulted in no significant difference in responses regarding viewers’ desires to own primates as pets or questions regarding the primates’ conservation statuses. Although most participants agreed the primates were endangered, more than 56% and 59%, respectively, stated they would have a gorilla or loris as a pet, that they would make good pets, or both, further supporting the conclusion that captions do not minimize harmful impacts of images of human−primate interactions.
Building references for nature conservation
Authors: Robert Alexandre 
Published: October 2023

AbstractConservation references have long been used in conservation biology to compare current biodiversity processes and states with past conditions. However, beyond the paucity of data for the construction of ancient, even prehuman, references, the relevance of these ancient references for studying ecosystems radically modified by human activities is questionable, particularly when the notions of conservation references and conservation objectives are confused and when several conservation ethics coexist that require distinct references. Because of this implicit heterogeneity in the nature of the references and their temporal baseline, conservation references not only have different meanings, but also deliver different messages. I propose establishing a common framework for conservation references to approach past biological systems and build comparable references between studies and projects. The selection of these references (distinct from conservation objectives) should be an early, explicit, standardized, and transparent milestone in any conservation process and these references should be based on state, pressure, or process dynamics, rather than fixed states. Finally, the importance of the diversity of temporal baselines used to build conservation references and to measure anthropogenic impacts should be recognized to understand the biodiversity crisis in its entirety.
Ratcheting behavior of surface‐modified AZ80 magnesium alloy after pre‐corrosion in simulated body fluids with various pH values
Authors: Xiong Ying , He Liuyong 
Published: October 2023

AbstractAZ80 magnesium alloy was surface treated by micro‐arc oxidation (MAO) and composite of laser shock penning (LSP) and MAO techniques. Untreated and treated specimens were pre‐corroded in simulated body fluids (SBF) with different pH values (pH 4, 7.4, and 9). The ratcheting behavior of all test specimens was experimentally investigated under stress‐controlled loading. The positive or negative value of the ratcheting strain reflects the mechanism of cyclic deformation. The ratcheting strain with negative values is associated with the cyclic deformation being dominated by twinning–detwinning, while the ratcheting strain with positive values is due to dislocation slip playing a dominant role in the cyclic deformation. A higher absolute value of the ratcheting strain means a lower the ratcheting life. Single MAO coating is not conducive to the fatigue life of magnesium alloy in acidic SBF environment, while LSP/MAO composite coating can improve the fatigue life of magnesium alloy under various loading conditions and different pH environment.
Agreement between one stage and chromogenic assays in samples from patients receiving recombinant porcine FVIII (Obizur, Susoctocog‐alfa)
Authors: Guy Susan , Bowyer Annette E. , Shepherd M. Fiona , Maclean Rhona M. , Kitchen Steve 
Published: October 2023

AbstractIntroductionRecombinant porcine FVIII (rpFVIII) (Obizur, Susoctcog‐alfa, Takeda, Japan) is licensed for the treatment of bleeding in acquired Haemophilia A (AHA). The summary of product characteristics state that monitoring should be by one stage assay (OSA) rather than chromogenic assay (CSA). CSA have been shown to underestimate activity when rpFVIII is added to plasma in vitro.MethodsSamples from three AHA patients (n = 21) (pre‐ and post rpFVIII) were assessed using FVIII:C assays; OSA methods: Actin, Actin FS, Actin FSL and Pathromtin SL performed on CS5100i (Sysmex, Kobe, Japan); APTT‐SP, SynthASil and SynthAFax performed on ACL TOP (Werfen, Barcelona, Spain). CSA methods on CS5100i: Siemens Chromogenic Assay, Biophen FVIII:C, Technochrom FVIII:C; on ACL TOP: Rox Factor VIII, Coamatic Factor VIII and CRYOcheck Factor VIII.ResultsOSA and CSA varied according to reagent used median OSA 61 IU/dL (range 41.5–81 IU/dL (ANOVA p < 0.0001)) median CSA 46.5 IU/dL (range of method specific medians 36.5–84 IU/dL (ANOVA p < 0.0001)). Amongst OSA, Actin FS was associated with the highest FVIII:C, APTT‐SP was associated with the lowest. Variation in CSA results by different methods was also seen with highest FVIII:C levels obtained using the Technochrom FVIII:C and the lowest levels obtained with Siemens Assay.ConclusionThe relationship between OSA and CSA was not consistent between method or patient. Previously there has been reports of underestimation by CSA in in vitro spiked samples. Investigation into concentration of phospholipids in the APTT reagents may explain some of these variations.
The effect of virtual reality learning on students' motivation: A scoping review
Authors: Jiang Juming , Fryer Luke K. 
Published: October 2023

AbstractBackgroundThe COVID‐19 pandemic has greatly impacted education, necessitating alternative methods to traditional face‐to‐face teaching. This dramatic change, in tandem with increasing awareness of the metaverse—a virtual reality (VR) world wherein humanity might learn, work and socialise–has made a clear need for a better understanding of the potential contribution of VR‐based simulation environments for students' learning outcomes.ObjectivesThree research questions regarding the effect of VR experience on students' learning motivation were addressed:RQ1. What methods have been employed to rule out the novelty effect in VR learning?RQ2. What motivation theories have been applied to guide the development of VR learning content?RQ3. What motivation theories have been applied to guide the utilisation of VR in learning activities?MethodsApplying Arksey and O'Malley's (International Journal of Social Research Methodology, 8, 19–32, 2005) protocol, 14 articles were included in this review.ResultsMost included studies indicated that students' motivation had increased after the VR intervention or was higher than other pedagogical conditions. However, the lack of longitudinal research designs means novelty effects in VR learning cannot be ruled out. Knowledge gaps in study designs and theoretical frameworks are discussed.ConclusionTechnology and the COVID‐19 pandemic are radically changing teaching and learning methods. More research on the impact of VR simulation learning on student outcomes is needed, with careful theoretical framing to ensure reliable findings. Future studies could explore VR technology to satisfy students' psychological needs and create deeper personal connections.
Determinants of dietary diversity and drivers of food choice among low‐income consumers in urban Kenya, Malawi and Zimbabwe
Authors: Gichohi‐Wainaina Wanjiku N. , Kee‐Tui Sabine Homann , Zoethout Manon , Talsma Elise F. , Edel Immaculate , Hauser Michael 
Published: October 2023

AbstractIntroductionRapid urbanisation affects lifestyle and eating habits, predominantly causing a dietary shift that adds challenges to meet dietary recommendations within a complex food system. This research investigated dietary diversity and food choice drivers among low‐income consumers in three urban settlements (Nairobi, Kenya; Bulawayo, Zimbabwe and Lilongwe, Malawi, representing rapid, moderate and slow urban growth patterns, respectively) as a first step towards improving diets across cultures and geographies.MethodsMixed methods data collection was employed for this study. Qualitative methods such as rapid foodscape appraisal workshops and food stories in selected low‐income settlements in Nairobi, Bulawayo and Lilongwe were utilised to collect information on the food environments (food balances, infrastructure, safety, policies and institutions). Quantitative data such as socio‐demographic characteristics and dietary diversity were collected via structured questionnaires using Cognitive Edge's SenseMaker® (n = 890, 450 and 440 for Nairobi, Bulawayo and Lilongwe, respectively). The dietary intake of respondents was assessed using a 24‐h recall, which was then converted to the food group diversity score (FGDS).ResultsDifferent levels of compounded stress affected dietary diversity and quality, including high food prices, concerns about sanitation and hygiene and the role of food standards regulation bodies. The mean FGDS across all locations was lower than the recommended cut‐off of 5 (4.5, 2.8 and 2.6 across Nairobi, Zimbabwe and Lilongwe, respectively). Additionally, in Nairobi, there were gender differences in diet diversity, with men having a higher dietary diversity score than women (4.6% vs. 4.3%; p = 0.004). The majority of respondents in Lilongwe (65%) reported price as the most important driver of food choice, compared to 38% in Nairobi and 42% in Bulawayo.Discussion/ConclusionsOur observation of poor‐quality diets provides further evidence of the need for food policies that are cognisant of the nutrition and health of the growing population of the urban poor. Such policies would focus on lowering the costs of nutritious foods as well as ensuring food safety within the complex food system observed in the urban low‐income environment.
Natural variation in STAYGREEN contributes to low‐temperature tolerance in cucumber
Authors: Dong Shaoyun , Li Caixia , Tian Haojie , Wang Weiping , Yang Xueyong , Beckles Diane M. , Liu Xiaoping , Guan Jiantao , Gu Xingfang , Sun Jiaqiang , Miao Han , Zhang Shengping 
Published: October 2023

AbstractLow‐temperature (LT) stress threatens cucumber production globally; however, the molecular mechanisms underlying LT tolerance in cucumber remain largely unknown. Here, using a genome‐wide association study (GWAS), we found a naturally occurring single nucleotide polymorphism (SNP) in the STAYGREEN (CsSGR) coding region at the gLTT5.1 locus associated with LT tolerance. Knockout mutants of CsSGR generated by clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR‐associated nuclease 9 exhibit enhanced LT tolerance, in particularly, increased chlorophyll (Chl) content and reduced reactive oxygen species (ROS) accumulation in response to LT. Moreover, the C‐repeat Binding Factor 1 (CsCBF1) transcription factor can directly activate the expression of CsSGR. We demonstrate that the LT‐sensitive haplotype CsSGRHapA, but not the LT‐tolerant haplotype CsSGRHapG could interact with NON‐YELLOW COLORING 1 (CsNYC1) to mediate Chl degradation. Geographic distribution of the CsSGR haplotypes indicated that the CsSGRHapG was selected in cucumber accessions from high latitudes, potentially contributing to LT tolerance during cucumber cold‐adaptation in these regions. CsSGR mutants also showed enhanced tolerance to salinity, water deficit, and Pseudoperonospora cubensis, thus CsSGR is an elite target gene for breeding cucumber varieties with broad‐spectrum stress tolerance. Collectively, our findings provide new insights into LT tolerance and will ultimately facilitate cucumber molecular breeding.
Pulmonary metastasectomy for recurrent metastatic ovarian cancer
Authors: Cheng Feng , Wang Junjian , Chen Qixun , Zhu Jianqing 
Published: October 2023

AbstractAimThe prognosis of patients with ovarian cancer with lung metastasis is poor; data on pulmonary metastasectomy for such patients are lacking. This study aimed to determine the safety and feasibility of pulmonary resection as part of cytoreductive surgery for recurrent metastatic ovarian cancer.MethodsMedical records of patients with ovarian cancer, who underwent pulmonary resection for lung metastasis in our hospital from April 2012 to February 2022, were retrospectively reviewed.ResultsTen patients were included (median age, 53 years). Five patients had metastatic disease limited to the lungs. Additional surgeries included diaphragm resection, partial hepatectomy, para‐aortic lymph node dissection, and cytoreduction. We achieved complete cytoreduction for all patients without severe complications, and the 30‐day mortality was zero. After a median follow‐up of 23 months, four of the patients experienced recurrence. One patient recurred 9 months after the operation and was lost to follow‐up at 17 months, two died at 68 and 26 months respectively, one is alive with disease (23 months), and six are alive without recurrence, among whom two have survived for 56 and 124 months.ConclusionsPulmonary resection for recurrent metastatic ovarian cancer seems safe and feasible, with long‐term survival observed in certain patients. Pulmonary metastasectomy can be performed as part of the debulking surgery for selected patients with relapsed metastatic ovarian cancer. Both the patient lost to follow‐up and the one who died at 26 months, had two lung metastatic nodules and did not receive postoperative chemotherapy, which might have led to relatively poor prognosis.
New Melastomataceae hosts of Chrysoporthe species in Brazil
Authors: Silva Gabrielle A. , Moreira Silvino I. , Ferreira Maria A. 
Published: October 2023

AbstractThe angiosperm Melastomataceae family is one of the most abundant plant families worldwide and in the Brazilian cerrado, with significant environmental importance in regenerating degraded areas, especially those previously occupied by pastures. Recently, Chrysoporthe Gryzenhout & M. J. Wingf. species were reported in Brazil, causing canker, branch dieback, and mortality in native Melastomataceae. This leads to the demand for further investigation and understanding of these pathosystems. During field surveys, typical signs and symptoms associated with Chrysoporthe infection were found in Rhynchanthera grandiflora (Aubl.) D C. and Miconia theaezans (Bonpl.) Cogn. in southern Minas Gerais. Through phylogenetic analysis of the BT1 and BT2 fragments of the β‐tubulin gene and morphological characterization of the isolates obtained, it was possible to identify C. doradensis Gryzenh. & M. J. Wingf. occurring in R. grandiflora and C. puriensis M. E. S. Oliv., T. P. F. Soar. & M. A. Ferr. occurring in R. grandiflora and M. theaezans. Furthermore, pathogenicity assays confirmed the pathogenicity of both species to their hosts.
Quantitative cellular changes in multiple system atrophy brains
Authors: Andersen Alberte M. , Kaalund Sanne S. , Marner Lisbeth , Salvesen Lisette , Pakkenberg Bente , Olesen Mikkel V. 
Published: October 2023

AbstractMultiple system atrophy (MSA) is a neurodegenerative disorder characterised by a combined symptomatology of parkinsonism, cerebellar ataxia, autonomic failure and corticospinal dysfunction. In brains of MSA patients, the hallmark lesion is the aggregation of misfolded alpha‐synuclein in oligodendrocytes. Even though the underlying pathological mechanisms remain poorly understood, the evidence suggests that alpha‐synuclein aggregation in oligodendrocytes may contribute to the neurodegeneration seen in MSA. The primary aim of this review is to summarise the published stereological data on the total number of neurons and glial cell subtypes (oligodendrocytes, astrocytes and microglia) and volumes in brains from MSA patients. Thus, we include in this review exclusively the reports of unbiased quantitative data from brain regions including the neocortex, nuclei of the cerebrum, the brainstem and the cerebellum. Furthermore, we compare and discuss the stereological results in the context of imaging findings and MSA symptomatology. In general, the stereological results agree with the common neuropathological findings of neurodegeneration and gliosis in brains from MSA patients and support a major loss of nigrostriatal neurons in MSA patients with predominant parkinsonism (MSA‐P), as well as olivopontocerebellar atrophy in MSA patients with predominant cerebellar ataxia (MSA‐C). Surprisingly, the reports indicate only a minor loss of oligodendrocytes in sub‐cortical regions of the cerebrum (glial cells not studied in the cerebellum) and negligible changes in brain volumes. In the past decades, the use of stereological methods has provided a vast amount of accurate information on cell numbers and volumes in the brains of MSA patients. Combining different techniques such as stereology and diagnostic imaging (e.g. MRI, PET and SPECT) with clinical data allows for a more detailed interdisciplinary understanding of the disease and illuminates the relationship between neuropathological changes and MSA symptomatology.
The European administrative space over time: Mapping the formal independence of EU agencies
Authors: Ruffing Eva , Weinrich Martin , Rittberger Berthold , Wonka Arndt 
Published: October 2023

AbstractThroughout the past decades, the EU's agency landscape has continuously expanded in size and scope. In this article, we address the lack of longitudinal data on EU agencies' formal independence. We introduce a newly revised index to measure the formal independence of EU agencies from other EU institutions over time. Applying a rules‐as‐data approach we coded 206 regulations and amendments to develop a new dataset covering the formal independence of all 39 EU agencies from 1975 to 2022. This longitudinal overview provides first insights about the development of formal independence at the case and population levels. At the case level we identify frequent, albeit gradual reforms of EU agencies' independence. At the population level, we observe remarkable stability in overall independence, but find stark variation across different independence dimensions. Overall, EU‐level principals have shifted over time from controlling individual decisions to controlling the agencies' general decision‐making apparatus.