bluuebunny/crossref_metadata_2025_split huggingface dataset:
Converging paths: Harnessing ensemble modelling to predict human wild pig conflict risk zones in Tamil Nadu
Authors: Shameer Thekke Thumbath , Routray Priyambada , Udhayan A , Ranjan Nihar , Ganesan Mannika Govindan , Manimozhi Arulmani , Kumari Dhayanithi Vasantha 
Published: December 2023

AbstractGrowing human populations and human activity intensify human-wildlife conflicts, particularly with wild pigs (Sus scrofa), necessitating consideration of both human and wildlife requirements. This complexity demands a comprehensive assessment of causes, impacts, and mitigation strategies for such conflicts. In order to address these issues, we conducted studies across the forest divisions of Tamil Nadu by collecting secondary data on conflicts from 2016 to 2021. Out of the 3301 incidents we collected, 94.4% were specifically related to crop damage and occurred mostly during the month of December, followed by November. Dharmapuri Forest Division was found to be the highest contributor to overall conflicts. Temporal analysis revealed conflict peaks in December, followed by November and September. Using ensemble modelling we predicted a conflict risk zone of approximately 49,223 km2, which represents 37.8% of the total area of Tamil Nadu. Variables like human modification index and mean annual temperature had the highest contribution to model performance. Our model’s projections indicate that areas with cultivated lands in close proximity to the fringes of forests, especially in regions where there is a greater degree of human modification, are associated with heightened levels of conflict risk. This study provides a concise overview of a comprehensive study on human-wild pig conflict, encompassing an exploration of conflict triggers and ecological impacts in the state of Tamil Nadu. The study offers a comprehensive roadmap for managing human-wild pig conflicts in Tamil Nadu, highlighting key triggers, ecological impacts, and conflict risk factors.
Multimodal Large Language Models are Generalist Medical Image Interpreters
Authors: Han Tianyu , Adams Lisa C. , Nebelung Sven , Kather Jakob Nikolas , Bressem Keno K. , Truhn Daniel 
Published: December 2023

AbstractMedicine is undergoing a transformation with the integration of Artificial Intelligence (AI). Traditional AI models, though clinically useful and often matching or surpassing expert clinicians in specific tasks, face a scalability challenge due to the necessity of developing individual models for each task. Therefore, there is a push towards foundation models that are applicable to a wider set of tasks. Our study showcases how non-domain-specific, publicly available vision-language models can be employed as general foundation models for medical applications. We test our paradigm across four medical disciplines - pathology, dermatology, ophthalmology, and radiology - focusing on two use-cases within each discipline. We find that our approach beats existing pre-training methods and is competitive to domain-specific foundation models that require vast amounts of domain-specific training images. We also find that large vision-language models are data efficient and do not require large annotated datasets to reach competitive performance. This allows for the development of new or improved AI models in areas of medicine where data is scarce and will accelerate medical progress towards true multimodal foundation models.
Mitochondria alterations in fibroblasts from sporadic Alzheimer’s disease (AD) patients correlate with AD-related clinical hallmarks
Authors: Eysert Fanny , Kinoshita Paula-Fernanda , Lagarde Julien , Lacas-Gervais Sandra , Xicota Laura , Dorothée Guillaume , Bottlaender Michel , Checler Frédéric , Potier Marie-Claude , Sarazin Marie , Chami Mounia 
Published: December 2023

ABSTRACTINTRODUCTIONMitochondria dysfunctions are key features in Alzheimer’s disease (AD). The occurrence of these disturbances in AD patient’s peripheral cells and their potential correlation with disease progression are under investigated.METHODSWe studied mitochondrial structure, function and mitophagy in fibroblasts including healthy volunteers and AD patients at the prodromal (AD-MCI) or demented (AD-D) stages. We carried out correlation studies with clinical cognitive scores, Aβ plaques burden and the accumulation of peripheral amyloid precursor protein C-terminal fragments (APP-CTFs).RESULTSWe unveiled progressive alteration of mitochondria structure as well as specific mitochondrial dysfunctions signatures in AD-MCI and AD-D fibroblasts and show defective mitophagy and autophagy linked to impaired lysosomal activity in AD-D fibroblasts. We reported on significant correlations of a subset of these dysfunctions with cognitive decline, AD-related clinical hallmarks and peripheral APP-CTFs accumulation.DISCUSSIONThis study emphasizes the potential use of peripheral cells for investigating AD pathophysiology and likely diagnosis.
Dynamic conformational changes of acid-sensing ion channels in different desensitizing conditions
Authors: Marcher Holm Caroline , Topaktas Asli B. , Dannesboe Johs , Pless Stephan A. , Heusser Stephanie A. 
Published: December 2023

AbstractAcid-sensing ion channels (ASICs) are proton-gated cation channels that contribute to fast synaptic transmission and have roles in fear conditioning and nociception. Apart from activation at low pH, ASIC1a also undergoes several types of desensitization, including ‘acute desensitization’ that terminates activation, ‘steady-stated desensitization’ that occurs at sub-activating proton concentrations and limits subsequent activation, and ‘tachyphylaxis’ that results in a progressive decrease in response during a series of activations. Structural insights from a desensitized state of ASIC1 have provided great spatial detail, but dynamic insights into conformational changes in different desensitizing conditions are largely missing. Here, we use electrophysiology and voltage-clamp fluorometry to follow the functional changes of the pore along with conformational changes at several positions in the extracellular and upper transmembrane domain via cysteine-labeled fluorophores. Acute desensitization terminates activation in wild-type but introducing an N414K mutation in the β11-12 linker of mouse ASIC1a interfered with this process. The mutation also affected steady-state desensitization and led to pronounced tachyphylaxis.Common to all types of desensitization was that the extracellular domain remained sensitive to pH and underwent pH-dependent conformational changes. These conformational changes did, however, not necessarily lead to desensitization. N414K-containing channels remained sensitive to known peptide modulators that increased steady-state desensitization, indicating that the mutation only reduced, but not precluded, desensitization. Together, this study contributes to understanding the fundamental properties of ASIC1a desensitization, emphasizing the complex interplay between the conformational changes of the ECD and the pore during channel activation and desensitization.Statement of significanceAcid-sensing ion channels (ASICs) are proton-gated ion channels that contribute to synaptic activity and play roles in acidosis-related diseases. Prolonged acidosis can lead to desensitization in ASIC1a, and modulators that affect this desensitization have shown beneficial effects in pain and stroke. In this study, we investigated the functional and conformational changes during acute desensitization, steady-state desensitization, and tachyphylaxis through a mutation in the β11-12 linker of ASIC1a. We found that the mutation retained pH-dependent conformational changes of the extracellular domain (ECD) but largely disconnected these movements from the channel pore. Collectively, our work emphasizes the critical role of the β11-12 linker for the pH-dependent conformational interplay between the ECD and the channel pore.
Iron depletion has different consequences on the growth and survival ofToxoplasma gondiistrains
Authors: Renaud Eléa A. , Maupin Ambre J.M. , Bordat Yann , Graindorge Arnault , Berry Laurence , Besteiro Sébastien 
Published: December 2023

AbstractToxoplasma gondiiis an obligate intracellular parasite that is responsible for a pathology called toxoplasmosis which is primarily affecting immunocompromised individuals and developing fetuses. The parasite is able to scavenge essential nutrients from its host to support its own growth and survival. Among them, iron is one of the most important elements needed to sustain basic cellular functions, as it is involved in a number of key metabolic processes, including oxygen transport, redox balance and electron transport. We have evaluated the effects of an iron chelator on the development of several parasite strains and found that they differed in their ability to tolerate iron depletion. The growth of parasites usually associated with a model of acute toxoplasmosis was strongly impacted by iron depletion, while cystogenic strains were less sensitive as they were able to convert into persisting developmental forms which are associated with the chronic form of the disease. Ultrastructural and biochemical characterization of the impact of iron depletion on the parasites also highlighted striking changes in both in their metabolism and the one of the host, with a marked accumulation of lipid droplets and perturbation of lipid homeostasis. Overall, our study demonstrates that although acute iron depletion has an important effect on the growth ofT. gondii, it has a more profound impact on actively dividing parasites, while less metabolically-active parasite forms may be able to avoid some of the most detrimental consequences.
Cyclin A/Cdk1 promotes chromosome alignment and timely mitotic progression
Authors: Valles Sarah Y. , Godek Kristina M. , Compton Duane A. 
Published: December 2023

ABSTRACTTo ensure genomic fidelity a series of spatially and temporally coordinated events are executed during prometaphase of mitosis, including bipolar spindle formation, chromosome attachment to spindle microtubules at kinetochores, the correction of erroneous kinetochore-microtubule (k-MT) attachments, and chromosome congression to the spindle equator. Cyclin A/Cdk1 kinase plays a key role in destabilizing k-MT attachments during prometaphase to promote correction of erroneous k-MT attachments. However, it is unknown if Cyclin A/Cdk1 kinase regulates other events during prometaphase. Here, we investigate additional roles of Cyclin A/Cdk1 in prometaphase by using an siRNA knockdown strategy to deplete endogenous Cyclin A from human cells. We find that depleting Cyclin A significantly extends mitotic duration, specifically prometaphase, because chromosome alignment is delayed. Unaligned chromosomes display erroneous monotelic, syntelic, or lateral k-MT attachments suggesting that bioriented k-MT attachment formation is delayed in the absence of Cyclin A. Mechanistically, chromosome alignment is likely impaired because the localization of the kinetochore proteins BUB1 kinase, KNL1, and MPS1 kinase are reduced in Cyclin A-depleted cells. Moreover, we find that Cyclin A promotes BUB1 kinetochore localization independently of its role in destabilizing k-MT attachments. Thus, Cyclin A/Cdk1 facilitates chromosome alignment during prometaphase to support timely mitotic progression.
Dissecting an ancient stress resistance trait syndrome in the compost yeastKluyveromyces marxianus
Authors: Christensen Kaylee E. , Duarte Abel , Ma Zhenzhen , Edwards Judith L. , Brem Rachel B. 
Published: December 2023

AbstractIn the search to understand how evolution builds new traits, ancient events are often the hardest to dissect. Species-unique traits pose a particular challenge for geneticists—cases in which a character arose long ago and, in the modern day, is conserved within a species, distinguishing it from reproductively isolated relatives. In this work, we have developed the budding yeast genusKluyveromycesas a model for mechanistic dissection of trait variation across species boundaries. Phenotypic profiling revealed robust heat and chemical-stress tolerance phenotypes that distinguished the compost yeastK. marxianusfrom the rest of the clade. We used culture-based, transcriptomic, and genetic approaches to characterize the metabolic requirements of theK. marxianustrait syndrome. We then generated a population-genomic resource forK. marxianusand harnessed it in molecular-evolution analyses, which found hundreds of housekeeping genes with evidence for adaptive protein variation unique to this species. Our data support a model in which, in the distant past,K. marxianusunderwent a vastly complex remodeling of its proteome to achieve stress resistance. Such a polygenic architecture, involving nucleotide-level allelic variation on a massive scale, is consistent with theoretical models of the mechanisms of long-term adaptation, and suggests principles of broad relevance for interspecies trait genetics.
Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle
Authors: Sava Ioana , Davis Luther J. , Gray Sally R. , Bright Nicholas A. , Luzio J. Paul 
Published: December 2023

AbstractRegulation of the luminal pH of late endocytic compartments in continuously fed mammalian cells is poorly understood. Using normal rat kidney fibroblasts, we investigated the reversible assembly/disassembly of the proton pumping V-ATPase when endolysosomes are formed by kissing and fusion of late endosomes with lysosomes and during the subsequent reformation of lysosomes. We took advantage of previous work showing that sucrosomes formed by the uptake of sucrose are swollen endolysosomes from which lysosomes are reformed after uptake of invertase. Using confocal microscopy and subcellular fractionation of NRK cells stably expressing fluorescently tagged proteins, we found net recruitment of the V1 subcomplex during sucrosome formation and loss during lysosome reformation, with a similar time course to RAB7a loss. Addition of invertase did not alter mTORC1 signalling, suggesting that the regulation of reversible V-ATPase assembly/disassembly in continuously fed cells differs from that in cells subject to amino acid depletion/re-feeding. Using live cell microscopy, we demonstrated recruitment of a fluorescently tagged V1 subunit during endolysosome formation and a dynamic equilibrium and rapid exchange between the cytosolic and membrane bound pools of this subunit. We conclude that reversible V-ATPase assembly/disassembly plays a key role in regulating endolysosomal/lysosomal pH in continuously fed cells.Significance statementIn continuously fed cells there is net recruitment of the V1 subcomplex of the proton pumping V-ATPase to endolysosomes as they are formed by kissing and fusion of late endosomes with lysosomes, reducing the luminal pH to promote the activity of lysosomal hydrolases.During lysosome reformation, alterations in mTORC1 signalling are not required for the net disassembly of the V-ATPase subcomplex, which occurs with a similar time course to loss of RAB7a.Alteration of the dynamic equilibrium and rapid exchange between the cytosolic and endolysosome-bound pools of the V1 subcomplex likely underlies the mechanism of V-ATPase assembly/disassembly.
Accounting, ESG dynamics and the pandemic: when the quality of disclosure becomes crucial to sustainable success
Authors: Murgolo Michael , Tettamanzi Patrizia , Minutiello Valentina 
Published: December 2023

Purpose
This study aims to investigate the quality of disclosure of a cutting-edge reporting tool – integrated reporting (<IR>) – in terms of its effectiveness to report on COVID-19 pandemic information, its ability to provide forward-looking information and risk impact implications, and its quality determinants in challenging times.

Design/methodology/approach
Thanks to a content analysis of 247 <IR> for FY20, an integrated reporting disclosure score was developed to assess the disclosure quality provided by the sampled companies. Three research questions were tested through logistic regressions.

Findings
Non-financial disclosure activities struggle to provide adequate information in terms of potential future scenarios, risk assessment and forward-looking analyses. However, companies incorporated in “Anglo-Saxon” territories drafted integrated reports of higher quality. More recently, incorporated companies have made a greater effort to measure and report COVID-19 pandemic impacts on environmental, social and governance and business activities, also increasing their risk assessment and mitigation efforts. Concerning the determinants of disclosure quality, leverage, corporate governance structures, country of incorporation and belonging to “high impact” industries all lead to a higher quality of <IR> disclosure.

Originality/value
Examining in detail corporate social responsibility activities and corporate governance integrity is pivotal to orienting strategy towards sustainable trajectories: to do so, corporate reporting and disclosure practices are essential tools. In this context, corporate governance systems that emphasize board diversity are proven, even in disruptive circumstances, to play a crucial role in providing corporate reports of higher quality. High disclosure quality that goes beyond mere financial results is considered to be necessary to remain competitive strategically, socially and environmentally.
A systematic review of artificial intelligence in managing climate risks of PPP infrastructure projects
Authors: Akomea-Frimpong Isaac , Dzagli Jacinta Rejoice Ama Delali , Eluerkeh Kenneth , Bonsu Franklina Boakyewaa , Opoku-Brafi Sabastina , Gyimah Samuel , Asuming Nana Ama Sika , Atibila David Wireko , Kukah Augustine Senanu 
Published: December 2023

PurposeRecent United Nations Climate Change Conferences recognise extreme climate change of heatwaves, floods and droughts as threatening risks to the resilience and success of public–private partnership (PPP) infrastructure projects. Such conferences together with available project reports and empirical studies recommend project managers and practitioners to adopt smart technologies and develop robust measures to tackle climate risk exposure. Comparatively, artificial intelligence (AI) risk management tools are better to mitigate climate risk, but it has been inadequately explored in the PPP sector. Thus, this study aims to explore the tools and roles of AI in climate risk management of PPP infrastructure projects.Design/methodology/approachSystematically, this study compiles and analyses 36 peer-reviewed journal articles sourced from Scopus, Web of Science, Google Scholar and PubMed.FindingsThe results demonstrate deep learning, building information modelling, robotic automations, remote sensors and fuzzy logic as major key AI-based risk models (tools) for PPP infrastructures. The roles of AI in climate risk management of PPPs include risk detection, analysis, controls and prediction.Research limitations/implicationsFor researchers, the findings provide relevant guide for further investigations into AI and climate risks within the PPP research domain.Practical implicationsThis article highlights the AI tools in mitigating climate crisis in PPP infrastructure management.Originality/valueThis article provides strong arguments for the utilisation of AI in understanding and managing numerous challenges related to climate change in PPP infrastructure projects.
Thinking fast and slow: a revised SOR model for an empirical examination of impulse buying at a luxury fashion outlet
Authors: Cao Dongmei , Meadows Maureen , Ma Xiao 
Published: December 2023

Purpose
Despite the extensive stimulus–organism–response (SOR) literature, little attention has been paid to the role of marketing activity as a key environmental stimulus, and there is a dearth of research examining the interplay between emotions and cognition on consumer behaviour, as well as the sequential effects of emotions on cognition. To address these gaps, this study aims to develop a revised SOR model by incorporating Kahneman’s fast and slow thinking theory to investigate the impulse buying of affordable luxury fashion (ALF).

Design/methodology/approach
The authors use outlet stores at Bicester village (BV) in England as the research context for ALF shopping. Partial least squares structural equation modelling was used to analyse a survey sample of 633 consumers with a BV shopping experience.

Findings
The authors find that impulse buying of ALF arises from the interplay of emotional and cognitive factors, as well as a sequential and dual process involving in-store stimuli affecting on-site emotion and in-store browsing.

Research limitations/implications
This study reveals that brand connection has a significant and negative influence on the relationship between on-site emotion and in-store browsing, advancing the SOR paradigm and reflecting the interactive effect of human emotion and reasoning on the impulse buying of ALF items.

Practical implications
Insights into consumers’ impulse buying offer practical implications for luxury brand management, specifically for ALF outlet retailers and store managers.

Originality/value
The results suggest a robust sequential effect of on-site emotion towards in-store browsing on impulse buying, providing updated empirical support for Kahneman’s theory of System 1 and System 2 thinking.
Time to imagine an escape: investigating the consumer timework at play in augmented reality
Authors: Preece Chloe , Skandalis Alexandros 
Published: December 2023

Purpose
While the spatial dimensions of augmented reality (AR) have received significant attention in the marketing literature, to date, there has been less consideration of its temporal dimensions. This paper aims to theorise digital timework through AR to understand a new form of consumption experience that offers short-lived, immersive forms of mundane, marketer-led escape from everyday life.

Design/methodology/approach
The authors draw upon Casey’s phenomenological work to explore the emergence of new dynamics of temporalisation through digitised play. An illustrative case study using AR shows how consumers use this temporalisation to find stability and comfort through projecting backwards (remembering) and forwards (imagining) in their lives.

Findings
The proliferation of novel digital technologies and platforms has radically transformed consumption experiences as the boundaries between the physical and the virtual, fantasy and reality and play and work have become increasingly blurred. The findings show how temporary escape is carved out within digital space and time, where controlled imaginings provide consumers with an illusion of control over their lives as they re-establish cohesion in a ruptured sense of time.

Research limitations/implications
The authors consider the more critical implications of the offloading capacity of AR, which they show does not prevent cognitive processes such as imagination and remembering but rather puts limits on them. The authors show that these more short-lived, everyday types of digitised escape do not allow for an escape from the structures of everyday life within the market, as much of the previous literature suggests.

Practical implications
The authors argue that corporations need to reflect upon the potential threats of immersive technologies such as AR in harming consumer escapism and take these into serious consideration as part of their strategic experiential design strategies to avoid leading to detrimental effects upon consumer well-being. More nuanced conceptualisations are required to unpack the antecedents of limiting people’s imagination and potentially limiting the fully fledged escape that consumers might desire.

Originality/value
Prior work has conceptualised AR as offloading the need for imagination by making the absent present. The authors critically unpack the implications of this for a more fluid understanding of the temporal logics and limits of consumer escapism.