bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for oral infection and dry socket post‐tooth extraction in medically complex patients in the absence of antibiotic prophylaxis: A case‐control study
Authors: Boyer Taylor L. , Solanki Pooja , McGregor Jessina C. , Wilson Geneva M. , Gibson Gretchen , Jurasic M. Marianne , Evans Charlesnika T. , Suda Katie J. 
Published: February 2024

AbstractIntroductionDry socket and infection are complications of tooth extractions. The objective was to determine risk factors for post‐extraction complications in patients without antibiotic prophylaxis stratified by early‐ and late‐complications and complication type (oral infection and dry socket).MethodsRetrospective, case (with complications)‐control (without complications) study of patients (n = 708) who had ≥1 extraction performed at any Veterans Health Administration facility between 2015–2019 and were not prescribed an antibiotic 30 days pre‐extraction.ResultsEarly complication cases (n = 109) were more likely to be female [odds ratio (OR) = 2.06; 95% confidence interval (CI):1.05–4.01], younger (OR = 0.29; 95% CI:0.09–0.94 patients ≥ 80 years old, reference:18–44 years), Native American/Alaska Native (OR = 21.11; 95% CI:2.33–191.41) and have fewer teeth extracted (OR = 0.53 3+ teeth extracted; 95% CI:0.31–0.88, reference:1 tooth extracted). Late complication cases (n = 67) were more likely to have a bipolar diagnosis (OR = 2.98; 95% CI:1.04–8.57), history of implant placement (OR = 8.27; 95% CI:1.63–41.82), and history of past smoking (OR = 2.23; 95% CI:1.28–3.88).ConclusionPredictors for post‐extraction complications among patients who did not receive antibiotic prophylaxis were similar to prior work in cohorts who received prophylaxis. Unique factors identified in a medically complex population included being younger, Native American/Alaska Native, having mental health conditions, history of a dental implant, and fewer teeth extracted.
Keeping and sharing secrets at the interpersonal level
Authors: Bedrov Alisa , Gable Shelly L. 
Published: February 2024

AbstractSecrets are inherently social, for they are always kept from somebody else. Accordingly, keeping and sharing personal secrets not only has implications for one’s close relationships, but the individual experience of keeping and sharing secrets is also largely influenced by existing close relationship dynamics. Here, we extend prior discussions of secrecy by providing a theoretical discussion of the sociality of secrets and the potential mechanisms through which they could influence and be influenced by interpersonal relationships. We specifically focus on the mechanisms of shared reality, authenticity, trust, and rumination, and argue that keeping and sharing secrets can have considerable influence on close relationships, in ways that may be distinct from self‐disclosure. This paper integrates individual‐level concerns about keeping and sharing secrets with the dyadic implications of those decisions by considering both the secret‐keeper and target (i.e., the person from whom the secret is kept or shared with). In turn, we offer novel predictions for future research regarding the interpersonal consequences of secrecy.
Next steps for workplace‐based assessments of entrustable professional activities
Authors: Stumbar Sarah E. , Eliason Sarah , Toonkel Rebecca , Amie Katrina , Bonnin Rodolfo , Gralnik Leonard , Kantor Julie , Minor Suzanne 
Published: February 2024

AbstractBackgroundFlorida International University Herbert Wertheim College of Medicine (FIU‐HWCOM) participated in the AAMC Core Entrustable Professional Activities (EPA) implementation pilot. Entrustment decision processes based on data from workplace‐based assessments (WBAs) were piloted. Outcomes illustrated challenges including variability across EPAs with regards to learner level alignment and feasibility of data collection in the form of WBAs. In addition, students reported discomfort requesting WBA completion by preceptors and dissatisfaction with associated feedback.ApproachTo guide future directions, we conducted a survey of third‐year students to better understand their experience with and perceptions of WBAs used to evaluate EPAs at FIU‐HWCOM.EvaluationSurvey response was 96% (n = 107/112). Most (84%) reported that WBAs were not valuable to their development and that preceptors often did not complete WBAs in a timely fashion. Many (47%) reported not receiving verbal feedback. Most students (78%) used language in written responses demonstrating confusion between the EPAs and the WBAs used to assess them.ImplicationsThe use of WBAs to assess EPAs did not have its intended impact at FIU‐HWCOM. For future classes, WBA forms will consist of paper cards with questions directly assessing performance of skills aligned with EPAs 1, 5 and 6 only. To continue to promote feedback, students will be required to collect WBAs on all clerkships, but the number of required WBAs will be less than prior and no entrustment decisions will be made.
Clinical outcomes of baloxavir versus oseltamivir in immunocompromised patients
Authors: Ringer Matthew , Malinis Maricar , McManus Dayna , Davis Matthew , Shah Sunish , Trubin Paul , Topal Jeffrey E. , Azar Marwan M. 
Published: February 2024

AbstractBackgroundNeuraminidase inhibitors, including oseltamivir, are the treatment standard for influenza. Baloxavir, a novel antiviral, demonstrated comparable outcomes to oseltamivir in outpatients with influenza. Baloxavir was equally effective as oseltamivir in a retrospective study of hospitalized patients with influenza at our institution. However, the efficacy of baloxavir in immunocompromised patients is unclear.MethodsWe conducted a retrospective cohort study of immunocompromised adult patients hospitalized with influenza A who received baloxavir from January 2019 to April 2019 or oseltamivir from January 2018 to April 2018. Demographic and clinical outcomes were assessed. Primary outcomes were time from antiviral initiation to resolution of hypoxia and fever. Secondary outcomes were length of stay (LOS), intensive care unit (ICU) care, ICU LOS, and 30‐day mortality.ResultsOf 95 total patients, 52 received baloxavir and 43 received oseltamivir. Other than younger age (57.5 vs. 65; p = .035) and longer duration between vaccination and symptom onset (114 vs. 86 days; p = .001) in the baloxavir group, baseline characteristics did not differ. H1 was the predominant subtype in the baloxavir group (65.3%) versus H3 in the oseltamivir group (85.7%). When comparing baloxavir to oseltamivir, there was no significant difference in median time from antiviral initiation to resolution of hypoxia (59.9 vs. 42.5 h) and to resolution of fever (21.6 vs. 26.6 h). There were no differences in secondary outcomes.ConclusionBaloxavir was not associated with longer time to resolution of hypoxia or fever in comparison to oseltamivir. Results must be taken in context of variations in seasonal influenza subtype and resistance rates.
image
A carboxysome‐based CO2 concentrating mechanism for C3 crop chloroplasts: advances and the road ahead
Authors: Nguyen Nghiem D. , Pulsford Sacha B. , Förster Britta , Rottet Sarah , Rourke Loraine , Long Benedict M. , Price G. Dean 
Published: February 2024

SUMMARYThe introduction of the carboxysome‐based CO2 concentrating mechanism (CCM) into crop plants has been modelled to significantly increase crop yields. This projection serves as motivation for pursuing this strategy to contribute to global food security. The successful implementation of this engineering challenge is reliant upon the transfer of a microcompartment that encapsulates cyanobacterial Rubisco, known as the carboxysome, alongside active bicarbonate transporters. To date, significant progress has been achieved with respect to understanding various aspects of the cyanobacterial CCM, and more recently, different components of the carboxysome have been successfully introduced into plant chloroplasts. In this Perspective piece, we summarise recent findings and offer new research avenues that will accelerate research in this field to ultimately and successfully introduce the carboxysome into crop plants for increased crop yields.
Gaza: A decolonial geography
Authors: Agha Zena , Esson James , Griffiths Mark , Joronen Mikko 
Published: February 2024

AbstractThis commentary addresses three objectives: (1) to situate and contextualise the ongoing military assault on Gaza within longer colonial histories in Palestine; (2) to collate resources that can equip geographers—specialist and non‐specialist, academic and non‐academic—with resources to build decolonial politics on Palestine–Israel; and (3) to contribute to discussions on what we, as geographers, can do to support Palestinian calls for liberation. These objectives are informed by a strong conviction that now is not a time for equivocation or silence. Palestinians, both in Palestine and in exile, need robust support and solidarity. This commentary offers one example of the form this support and solidarity can take in the context of academia. It does so by calling forth our discipline's plural and deep commitment to justice and thoroughgoing critique of the deleterious power structures and colonial legacies that have brought us to this current moment of crisis in Palestine.
Diagnostic utility of the total nucleated cell count for differentiation of septic and sterile peritoneal effusions in dogs
Authors: DiDomenico Amy E. , Jacob Megan E. , Stowe Devorah M. , Gruber Erika J. 
Published: February 2024

AbstractBackgroundRapid and accurate diagnosis of septic peritonitis is critical for initiating appropriate medical and surgical management.ObjectivesThe aim of this study was to determine the diagnostic utility of the total nucleated cell count (TNCC), absolute neutrophil count, neutrophil percentage, and total protein (TP) to distinguish septic versus non‐septic peritoneal effusions in dogs.MethodsElectronic medical records were retrospectively searched for peritoneal fluid samples from 2008 to 2018 and classified as septic or non‐septic based on bacterial culture and/or cytology results. Receiver operator characteristic curves (ROCs) were used to describe the overall diagnostic utility of each test, with optimal cutpoints analyzed to dichotomize continuous variables. Positive and negative likelihood ratios were calculated at these cutpoints.ResultsA total of 166 unique samples, including 87 septic and 79 non‐septic peritoneal effusions, were included. There were no significant differences in dog sex, age, or days hospitalized between groups. Septic effusions had significantly higher TP, TNCC, absolute neutrophil count, and neutrophil percentage compared with non‐septic effusions. The area under the curve of the ROC curves was TNCC (0.80), absolute neutrophil count (0.80), neutrophil percentage (0.64), and TP (0.63). For TNCC and absolute neutrophil count, optimal cutoffs were 17.13 × 103 cells/μL and 19.88 × 103 cells/μL, resulting in positive and negative likelihood ratios of 2.39 and 0.28 and 2.85 and 0.28, respectively.ConclusionsTotal nucleated cell counts and absolute neutrophil counts aid in the differentiation of septic and non‐septic peritoneal effusions with similar diagnostic utility but are not sufficiently sensitive or specific to use without concurrent microscopic evaluation.
Algebraically generated groups and their Lie algebras
Authors: Kraft Hanspeter , Zaidenberg Mikhail 
Published: February 2024

AbstractThe automorphism group of an affine variety is an ind‐group. Its Lie algebra is canonically embedded into the Lie algebra of vector fields on . We study the relations between subgroups of and Lie subalgebras of . We show that a subgroup generated by a family of connected algebraic subgroups of is algebraic if and only if the Lie algebras generate a finite‐dimensional Lie subalgebra of . Extending a result by Cohen–Draisma (Transform. Groups 8 (2003), no. 1, 51–68), we prove that a locally finite Lie algebra generated by locally nilpotent vector fields is algebraic, that is, for an algebraic subgroup . Along the same lines, we prove that if a subgroup generated by finitely many connected algebraic groups is solvable, then it is an algebraic group. We also show that a unipotent algebraic subgroup has derived length . This result is based on the following triangulation theorem: Every unipotent algebraic subgroup of with a dense orbit in is conjugate to a subgroup of the de Jonquières subgroup. Furthermore, we give an example of a free subgroup generated by two algebraic elements such that the Zariski closure is a free product of two nested commutative closed unipotent ind‐subgroups. To any affine ind‐group , one can associate a canonical ideal . It is linearly generated by the tangent spaces for all algebraic subsets that are smooth in . It has the important property that for a surjective homomorphism , the induced homomorphism is surjective as well. Moreover, if is a subnormal closed ind‐subgroup of finite codimension, then has finite codimension in .
Multilinear rough singular integral operators
Authors: Grafakos Loukas , He Danqing , Honzík Petr , Park Bae Jun 
Published: February 2024

AbstractWe study ‐linear homogeneous rough singular integral operators associated with integrable functions on with mean value zero. We prove boundedness for from to when and in the largest possible open set of exponents when and . This set can be described by a convex polyhedron in .
Pre‐intervention myocardial stress is a good predictor of aortic valvuloplasty outcome for fetal critical aortic stenosis and evolving HLHS
Authors: Green Laura , Chan Wei Xuan , Prakash Indumita , Tulzer Andreas , Tulzer Gerald , Yap Choon Hwai 
Published: February 2024

AbstractFetal critical aortic stenosis with evolving hypoplastic left heart syndrome (CAS‐eHLHS) causes biomechanical and functional aberrations, leading to a high risk of progression to hypoplastic left heart syndrome (HLHS) at birth. Fetal aortic valvuloplasty (FAV) can resolve outflow obstruction and may reduce progression risk. However, it is currently difficult to accurately predict which patients will respond to the intervention and become functionally biventricular (BV) at birth, as opposed to becoming functionally univentricular (UV). This prediction is important for patient selection, parental counselling, and surgical planning. Therefore, we investigated whether biomechanics parameters from pre‐FAV image‐based computations could robustly distinguish between CAS‐eHLHS cases with BV or UV outcomes in a retrospective cohort. To do so we performed image‐based finite element biomechanics modelling of nine CAS‐eHLHS cases undergoing intervention and six healthy fetal control hearts, and found that a biomechanical parameter, peak systolic myofibre stress, showed a uniquely large difference between BV and UV cases, which had a larger magnitude effect than echocardiography parameters. A simplified equation was derived for quick and easy estimation of myofibre stress from echo measurements via principal component analysis. When tested on a retrospective cohort of 37 CAS‐eHLHS cases, the parameter outperformed other parameters in predicting UV versus BV outcomes, and thus has a high potential of improving outcome predictions, if incorporated into patient selection procedures. Physiologically, high myocardial stresses likely indicate a healthier myocardium that can withstand high stresses and resist pathological remodelling, which can explain why it is a good predictor of BV outcomes.
imageKey points
Predicting the morphological birth outcomes (univentricular versus biventricular) of fetal aortic valvuloplasty for fetal aortic stenosis with evolving HLHS is important for accurate patient selection, parental counselling and management decisions.
Computational simulations show that a biomechanics parameter, pre‐intervention peak systolic myofibre stress, is uniquely robust in distinguishing between such outcomes, outperforming all echo parameters.
An empirical equation was developed to quickly compute peak systolic myofibre stress from routine echo measurements and was the best predictor of outcomes among a wide range of parameters tested.
Building a Partnership Between a University and Local High School to Foster and Grow Interest in Biomedical Sciences and Engineering
Authors: Castile Ryan M. , Jobe Jamie , Iannucci Leanne E. , Reals Rebecca F. , Pavey Shawn N. , Fitzgerald Jon , Lake Spencer P. 
Published: February 2024

Abstract
To help foster interest in science, technology, engineering, and math (STEM), it is important to develop opportunities that excite and teach young minds about STEM-related fields. Over the past several years, our university-based research group has sought to help grow excitement around the biomechanics and biomedical engineering fields. The purposes of this technical brief are to (1) discuss the development of a partnership built between a St. Louis area high school and biomechanics research lab and (2) provide practical guidance for other researchers looking to implement a long-term outreach program. The partnership uses three different outreach opportunities. The first opportunity consisted of 12th-grade students visiting university research labs for an up-close perspective of ongoing biomedical research. The second opportunity was a biomedical research showcase where research-active graduate students traveled to the high school to perform demonstrations. The third opportunity consisted of a collaborative capstone project where a high school student was able to carry out research directly in a university lab. To date, we have expanded our reach from 19 students to interacting with over 100 students, which has yielded increased interest in STEM related research. Our postprogram survey showed that outreach programs such as the one described herein can increase interest in STEM within all ages of high school students. Building partnerships between high schools and university researchers increases the interest in STEM amongst high school students, and gives graduate students an outlet to present work to an eager-to-learn audience.
Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression
Authors: Zhao Yanqun , Liu Wei , Li Yanjie , Ma Jing , Liu Ting , Cui Huichan , Deng Yongheng , Liao Xiaochen , Wang Zekun 
Published: February 2024

ABSTRACT

Adeno-associated virus (AAV) requires co-infection with helper virus for efficient replication. We previously reported that Human Bocavirus 1 (HBoV1) genes, including
NP1
,
NS2
, and
BocaSR
, were critical for AAV2 replication. Here, we first demonstrate the essential roles of the NP1 protein in AAV2 DNA replication and protein expression. We show that NP1 binds to single-strand DNA (ssDNA) at least 30 nucleotides (nt) in length in a sequence-independent manner. Furthermore, NP1 colocalized with the BrdU-labeled AAV2 DNA replication center, and the loss of the ssDNA-binding ability of NP1 by site-directed mutation completely abolished AAV2 DNA replication. We used affinity-tagged NP1 protein to identify host cellular proteins associated with NP1 in cells cotransfected with the HBoV1 helper genes and AAV2 duplex genome. Of the identified proteins, we demonstrate that NP1 directly binds to the DBD-F domain of the RPA70 subunit with a high affinity through the residues 101–121. By reconstituting the heterotrimer protein RPA
in vitro
using gel filtration, we demonstrate that NP1 physically associates with RPA to form a heterologous complex characterized by typical fast-on/fast-off kinetics. Following a dominant-negative strategy, we found that NP1-RPA complex mainly plays a role in expressing AAV2 capsid protein by enhancing the transcriptional activity of the p40 promoter. Our study revealed a novel mechanism by which HBoV1 NP1 protein supports AAV2 DNA replication and capsid protein expression through its ssDNA-binding ability and direct interaction with RPA, respectively.

IMPORTANCE

Recombinant adeno-associated virus (rAAV) vectors have been extensively used in clinical gene therapy strategies. However, a limitation of these gene therapy strategies is the efficient production of the required vectors, as AAV alone is replication-deficient in the host cells. HBoV1 provides the simplest AAV2 helper genes consisting of
NP1
,
NS2
, and
BocaSR
. An important question regarding the helper function of HBoV1 is whether it provides any direct function that supports AAV2 DNA replication and protein expression. Also of interest is how HBoV1 interplays with potential host factors to constitute a permissive environment for AAV2 replication. Our studies revealed that the multifunctional protein NP1 plays important roles in AAV2 DNA replication via its sequence-independent ssDNA-binding ability and in regulating AAV2 capsid protein expression by physically interacting with host protein RPA. Our findings present theoretical guidance for the future application of the HBoV1 helper genes in the rAAV vector production.
Structural insights into the interaction between adenovirus C5 hexon and human lactoferrin
Authors: Dhillon Arun , Persson B. David , Volkov Alexander N. , Sülzen Hagen , Kádek Alan , Pompach Petr , Kereïche Sami , Lepšík Martin , Danskog Katarina , Uetrecht Charlotte , Arnberg Niklas , Zoll Sebastian 
Published: February 2024

ABSTRACT

Adenovirus (AdV) infection of the respiratory epithelium is common but poorly understood. Human AdV species C types, such as HAdV-C5, utilize the Coxsackie-adenovirus receptor (CAR) for attachment and subsequently integrins for entry. CAR and integrins are however located deep within the tight junctions in the mucosa where they would not be easily accessible. Recently, a model for CAR-independent AdV entry was proposed. In this model, human lactoferrin (hLF), an innate immune protein, aids the viral uptake into epithelial cells by mediating interactions between the major capsid protein, hexon, and yet unknown host cellular receptor(s). However, a detailed understanding of the molecular interactions driving this mechanism is lacking. Here, we present a new cryo-EM structure of HAdV-5C hexon at high resolution alongside a hybrid structure of HAdV-5C hexon complexed with human lactoferrin (hLF). These structures reveal the molecular determinants of the interaction between hLF and HAdV-C5 hexon. hLF engages hexon primarily
via
its N-terminal lactoferricin (Lfcin) region, interacting with hexon’s hypervariable region 1 (HVR-1). Mutational analyses pinpoint critical Lfcin contacts and also identify additional regions within hLF that critically contribute to hexon binding. Our study sheds more light on the intricate mechanism by which HAdV-C5 utilizes soluble hLF/Lfcin for cellular entry. These findings hold promise for advancing gene therapy applications and inform vaccine development.

IMPORTANCE
Our study delves into the structural aspects of adenovirus (AdV) infections, specifically HAdV-C5 in the respiratory epithelium. It uncovers the molecular details of a novel pathway where human lactoferrin (hLF) interacts with the major capsid protein, hexon, facilitating viral entry, and bypassing traditional receptors such as CAR and integrins. The study’s cryo-EM structures reveal how hLF engages hexon, primarily through its N-terminal lactoferricin (Lfcin) region and hexon’s hypervariable region 1 (HVR-1). Mutational analyses identify critical Lfcin contacts and other regions within hLF vital for hexon binding. This structural insight sheds light on HAdV-C5’s mechanism of utilizing soluble hLF/Lfcin for cellular entry, holding promise for gene therapy and vaccine development advancements in adenovirus research.