bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of evidence supporting the role of hyperbaric oxygen therapy for diabetic foot ulcers
Authors: Jiang Fuxin , Zhang Yalan , Cheng Shijin , Yang Xiaohui , Bai Min , Zhang Ming 
Published: December 2023

AbstractThe goal of this overview of systematic reviews (SRs) and meta‐analyses (MAs) was to methodically gather, evaluate and summarize the data supporting the use of hyperbaric oxygen therapy (HBOT) to treat diabetic foot ulcers (DFUs). The Cochrane Library, Embase, PubMed, Web of Science and Embase were all searched thoroughly to identify SRs/MAs that qualified. AMSTAR‐2 tool, PRISMA checklists and GRADE system were applied by two reviewers independently to assess the methodological quality, reporting and evidence quality of the included SRs/MAs, respectively. Eleven SRs/MAs were enrolled in this overview. According to AMSTAR‐2, a very low methodological quality assessment was given to the included SRs/MAs due to the limitations of items 2, 4 and 7. For the PRISMA, the overall quality of reporting is not satisfactory due to missing reporting on protocol, search, as well as additional analysis. The majority of outcomes had low‐ to moderate‐quality evidence, and no high‐quality evidence was found to support the role of HBOT for DFUs, according to GRADE. To conclude, the potential of HBOT in treating DFUs is supported by evidence of low to moderate quality. More rigorously designed, high‐level studies are needed in the future to determine the evidence for HBOT for DFU, including the timing, frequency and duration of HBOT interventions.
Novel approach for manufacture of single‐grain EuBCO/Ag bulk superconductors via modified single‐direction melt growth
Authors: Antončík Filip , Lojka Michal , Hlásek Tomáš , Sedmidubský David , Baumann Josef , Durrell John H. , Cardwell David A. , Jankovský Ondřej 
Published: December 2023

AbstractDespite the success of top‐seeded melt growth (TSMG) and TSIG, some key aspects of their manufacture need to be addressed including the presence of microstructural defects, inhomogeneities in trapped field and the difficulty of growing large‐diameter bulk monoliths. Recently, a promising new method single‐direction melt growth (SDMG) appears to address the majority of the shortcomings of TSMG and TSIG. Single‐grain EuBCO/Ag bulk with the highest peritectic temperature to date was grown by modified SDMG. This remarkable achievement was made possible by lowering the peritectic temperature of the precursor composition by the addition of silver and by using single‐grain EuBCO fabricated by TSMG as a seed. The EuBCO/Ag bulk grown by SDMG has demonstrated significant improvements in comparison to a reference TSMG sample. A reduction of approximately 30% in defect area (associated primarily with porosity), as well as a significantly enhanced homogeneity of trapped field and critical temperature, was observed. Furthermore, the critical current density curves exhibit a considerable improvement, particularly in the 2–5 T field range. The SDMG technique has considerable potential for scale‐up for the fabrication of large‐diameter bulk single grains, with only growth along the c‐axis involved during the thermal process.
Association between SPRY1 and TET3 in skin photoaging and natural aging mechanisms
Authors: Qiu Bo , Yang E. , Zheng Yulian , Zhang Hengshu 
Published: December 2023

AbstractBackgroundSPRY1 is associated with the invasiveness and prognosis of various tumors, and TET3 affects aging by regulating gene expression.AimsWe investigated the roles of SPRY1 and TET3 in natural skin aging, replicative aging, and photoaging, along with the effect of UVA on genome‐wide DNA methylation in HaCaT cells.MethodsTET3 and SPRY1 expression were measured in the skin of patients of different age groups, as well as in vitro human skin, HaCaT cell replicative senescence, and HaCaT and HaCaT‐siTET3 cell photoaging models. Senescence was verified using β‐galactosidase staining, and DNA damage was detected using immunofluorescence staining for γ‐H2A.X. 5‐Methyl cytosine (5‐mC) content in the genome was determined using ELISA.ResultsSPRY1 expression increased with age, whereas TET3 expression decreased. Similarly, SPRY1 was upregulated and TET3 was downregulated with increasing cell passages. TET3‐siRNA upregulated SPRY1 expression in HaCaT cells. UVA irradiation promoted HaCaT cell senescence and induced cellular DNA damage. SPRY1 was upregulated and TET3 was downregulated upon UVA irradiation. Genome‐wide 5‐mC content increased upon TET3 silencing and UVA irradiation, indicating a surge in overall methylation.ConclusionsSPRY1 and TET3 are natural skin aging‐related genes that counteract to regulate replicative aging and UVA‐induced photoaging in HaCaT cells. The cell photoaging model may limit experimental bias caused by different exposure times of skin model samples.
A review on ESG investing: Investors’ expectations, beliefs and perceptions
Authors: Kräussl Roman , Oladiran Tobi , Stefanova Denitsa 
Published: December 2023

AbstractThis study examines the recent literature on the expectations, beliefs and perceptions of investors who incorporate Environmental, Social, Governance (ESG) considerations in investment decisions with the aim to generate superior performance or make a societal impact. Through the lens of equilibrium models of agents with heterogeneous tastes for ESG investments, green assets are expected to generate lower returns in the long run compared to their non‐ESG counterparts. However, in the short run, ESG investments can outperform non‐ESG investments through various channels. Empirically, results for the relative performance to ESG investment are mixed. We find strong empirical evidence in the literature that investors have a preference for ESG and that their actions can generate positive social impact through engagement. The shift towards more sustainable policies in firms is motivated by the increased market values and the lower cost of capital of green firms driven by investors’ choices.
Printed abutment model to limit residual cement: A dental technique
Authors: Ryser Andrew S. , Sabol Jennifer V. , Handel Steven 
Published: December 2023

AbstractPreventing retained cement around implant‐retained crowns is crucial for the long‐term health and success of the implant. Residual subgingival cement is strongly associated with peri‐mucositis and can lead to peri‐implantitis. This clinical technique utilizes a 3D printed model of a custom abutment to seat the implant crown on before cementing intra‐orally to extrude excess cement. The technique aims to limit the amount of retained cement at the crown‐abutment margin and also takes into consideration the cement space already designed into the crown‐abutment complex.
Spatial and temporal distribution of hydrocarbon production on the UK continental shelf
Authors: Osah Ukari , Howell John 
Published: December 2023

AbstractHydrocarbon production from oil and gas fields is controlled by a variety of interconnected factors with a hierarchy of significance that is, for the most part, difficult to untangle. This article documents and investigates the spatial and temporal distribution of key hydrocarbon field parameters on the UK Continental Shelf. Data have been compiled from publicly available sources for 424 fields. Variables are considered as “descriptive parameters,” “control parameters” and “outcomes”. Descriptive parameters are metadata such as field name, location, etc. Control Parameters include depositional environment, present depth of burial, porosity, permeability, reservoir formation pressure, reservoir temperature, average net‐to‐gross, number of fault populations, hydrocarbon API, field area, bulk rock volume, well density, number of wells (production and injection), well spacing, gas oil ratio, reservoir thickness, fluid saturation, compartmentalization (quantitated by number of observable non‐communicating fault compartments), structural complexity (scaled from 0 to 5), field production strategy, trap type and stratigraphic heterogeneity. Outcomes are used to assess field performance and include final recovery factor (estimated), maximum production rate, and cumulative monthly production. Analysis of the database illustrates a number of empirical observations regarding hydrocarbon production on the UKCS. The Jurassic plays have been the most successful in the region in terms of total volumes produced while the Permian reservoirs of the SNS account for the majority of the gas. Most of the UKCS reservoirs record top depths between 2000 and 4500 m with good reservoir quality. The best reservoir quality is observed in reservoirs that were deposited within deep marine systems. The largest hydrocarbon reserves are found in the Northern North Sea basin in these deep marine (as well as paralic and shallow marine) reservoirs. Using the data from this article and affiliated data, potential exists for extracting insight beyond spatio‐temporal distributions.
São Paulo's Crackland as Urban Impasse: An Ethnographic Account of Mobility, Territory and Viracao as Form of Nomadism
Authors: Fromm Deborah , Blokland Talja 
Published: December 2023

AbstractDrugs feature in the geography of crime as an economy and as a threat to social order and public health. Spatial and social strategies of crime reduction ascribe agency to the state and to regulated residents of marginalised urban areas. Geographers also discussed anti‐drug policy have a revanchist neoliberal governance. Through the lens of urban territory and urban nomads in the crack‐cocaine‐dominated area Crackland in the centre of São Paulo, we argue that instead of a fixity of homelessness, drug markets and drug users in the geography of crime, one could pay more attention to nomadism, which can be found ethnographically in the journeys of three agents which we discuss. For over 20 years, city authorities have tried to ‘end’ Crackland with social programmes and police actions, or codifying attempts of territorialisation. An urban impasse emerged as these programmes failed to recognise Crackland as mobile territory of viracao. Crackland remains a zone reserved for the city's ‘human waste’ while perfectly integrated in the urban fabric.
Clarifying contemporary conceptualizations of allyship with LGBTQ2S+ groups in the context of health care or health settings: a scoping review protocol
Authors: Handlovsky Ingrid , Marcellus Lenora , Newton Lorelei , Zakher Bernadette , Mussell Jessica 
Published: December 2023

Objective:
The objective of this review is to better understand how allyship is defined in the literature from 1970 to the present with regard to lesbian, gay, bisexual, transgender, queer, two-spirit, and other (LGBTQ2S+) groups within health settings where English is the primary spoken language.

Introduction:
LGBTQ2S+ individuals experience health inequities rooted in discrimination. Activism to redress this discrimination in health settings is frequently termed allyship. Definitions of allyship, however, remain ambiguous. A clearer understanding of how allyship is defined and operationalized within health settings is integral to supporting the health of LGBTQ2S+ groups.

Inclusion criteria:
Literature in English from 1970 to the present that utilizes the concept of allyship within health care and/or health settings in relation to LGBTQ2S+ groups in Canada and the United States, Australia, New Zealand, and the United Kingdom will be included.

Methods:
This scoping review will be conducted in accordance with the JBI methodology for scoping reviews. Databases to be searched will include MEDLINE (OVID), CINAHL (EBSCOhost), APA PsycINFO (EBSCOhost), LGBTQ+ Source (EBSCOhost), Scopus, and Web of Science, along with ProQuest Dissertations and Theses for gray literature. Two independent reviewers will screen titles, abstracts, and full-text articles; discrepancies will be resolved by consensus or with a third reviewer. Data will be extracted using an extraction tool developed by the research team. Findings will be presented in tabular/diagram format along with a narrative summary to highlight key themes that relate to contemporary conceptualizations of allyship with LGBTQ2S+ individuals/groups within health care settings and the implications for health professional practice and health outcomes.

Review registration:
Open Science Framework osf.io/2rek9
Complete genome sequence of
Methanothermobacter
sp. DP, a hydrogenotrophic and thermophilic methanogen
Authors: Prabhaharan Darsha , Nair Pranav Sasidharan , Park Hyojung , Choi Okkyoung , Sang Byoung-In 
Published: December 2023

ABSTRACT

Here, we report the complete genome sequence of the thermophilic hydrogenotrophic methanogen
Methanothermobacter
sp. DP isolated in South Korea from an anaerobic digester fed with cigarette waste. The genome consists of 1,693,285 bp, with 1,772 protein-coding genes and a GC content of 48.8%.
Host DNA depletion methods and genome-centric metagenomics of bovine hindmilk microbiome
Authors: Duarte Vinícius da Silva , Porcellato Davide 
Published: December 2023

ABSTRACT
Bovine mastitis is a multi-etiological and complex disease, resulting in serious economic consequences for dairy farmers and industry. In recent years, the microbiological evaluation of raw milk has been investigated in-depth using next-generation sequencing approaches such as metataxonomic analysis. Despite this, host DNA is a major concern in the shotgun metagenomic sequencing of microbial communities in milk samples, and it represents a big challenge. In this study, we aimed to evaluate different methods for host DNA depletion and/or microbial DNA enrichment and assess the use of PCR-based whole genome amplification in milk samples with high somatic cell count (SCC) by using short- and long-read sequencing technologies. Our results evidenced that DNA extraction performed differently in terms of host DNA removal, impacting metagenome composition and functional profiles.. Moreover, the ratio of SCC/bacteria ultimately impacts microbial DNA yield, and samples with low SCC (SCC below 100,000 cells/mL) are the most problematic. When milk samples with high SCC (SCC above 200,000 cells/mL) underwent multiple-displacement amplification (MDA), we successfully recovered high-quality metagenome-assembled genomes (MAGs), and long-read sequencing was feasible even for samples with low DNA concentration. By associating MDA and short‐read sequencing, we recovered two times more MAGs than in untreated samples, and an ongoing co‐infection not reported by traditional methods was detected for mastitis pathogen. Overall, this new approach will improve the detection of mastitis-associated microorganisms and make it possible to examine host-microbiome interactions in bovine mastitis.

IMPORTANCE
Next-generation sequencing technologies have been widely used to gain new insights into the diversity of the microbial community of milk samples and dairy products for different purposes such as microbial safety, profiling of starter cultures, and host-microbiome interactions. Milk is a complex food matrix, and additionally, the presence of host nucleic acid sequences is considered a contaminant in untargeted high-throughput sequencing studies. Therefore, genomic‐centric metagenomic studies of milk samples focusing on the health‐disease status in dairy cattle are still scarce, which makes it difficult to evaluate the microbial ecophysiology of bovine hindmilk. This study provides an alternative method for genome-centric metagenome studies applied to hindmilk samples with high somatic cell content, which is indispensable to examining host-microbiome interactions in bovine mastitis.
Construction of an arrayed CRISPRi library as a resource for essential gene function studies in
Streptococcus mutans
Authors: St. Pierre Jackson , Roberts Justin , Alam Mohammad A. , Shields Robert C. 
Published: December 2023

ABSTRACT

Arrayed mutant libraries are important resources that have advanced our understanding of bacterial genetics. Until recently, essential genes which are necessary for bacterial survival, and constitute ~10% of bacterial genes, were not included in arrayed library resources. However, recent advances in clustered regularly interspaced short palindromic repeats interference (CRISPRi) have made it possible to study essential genes with increasing throughput. Here, we constructed an arrayed CRISPRi library in the dental caries pathogen
Streptococcus mutans
, known as SNAP (

S

treptococcus muta
n
s

a
rrayed CRIS
P
Ri). In this library, each strain contains a short guide RNA which selectively targets a single essential gene, and this is controlled through xylose induction. In total, the library can selectively repress >250 essential and growth-supporting genes. Initial characterization showed that SNAP strains grow as expected, and initial proof-of-concept experiments displayed the utility of the library. We anticipate that this library will be of benefit to the research community by allowing for high-throughput characterization of
S. mutans
essential genes.

IMPORTANCE

The construction of arrayed mutant libraries has advanced the field of bacterial genetics by allowing researchers to more efficiently study the exact function and importance of encoded genes. In this study, we constructed an arrayed clustered regularly interspaced short palindromic repeats interference (CRISPRi) library, known as

S

treptococcus muta
n
s

a
rrayed CRIS
P
Ri (SNAP), as a resource to study >250 essential and growth-supporting genes in
Streptococcus mutans
. SNAP will be made available to the research community, and we anticipate that its distribution will lead to high-quality, high-throughput, and reproducible studies of essential genes.
A multichromatic colorimetric detection method for
Vibrio parahaemolyticus
based on Fe
3
O
4
-Zn-Mn nanoenzyme and dual substrates
Authors: Qiao Wenteng , Wang Luliang , Yang Kun , Liu Yushen , Liu Quanwen , Yin Feng 
Published: December 2023

ABSTRACT

A successful development of a dual-substrate colorimetric system for rapid and multicolorimetric semi-quantification of
Vibrio parahaemolyticus (V. parahaemolyticus
) has been achieved. The enzymatic activity of Fe
3
O
4
-Zn-MnO
2
-aptamer (Fe
3
O
4
-Zn-Mn-Apt) catalysts can induce the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form oxidized TMB (TMB
+
with a bluish green color) and ortho-phenylenediamine (OPD) to form oxidized OPD (with yellow color), resulting in a three-color comparison due to the complementary nature of bluish green and yellow. The absorbance value of TMB
+
at 652 nm is used for the determination of
V. parahaemolyticus
. After optimizing the reaction conditions, the developed dual-substrate colorimetric method exhibits high sensitivity for
V. parahaemolyticus
detection (limit of detection of 1.12 cfu mL
−1
) and a linear range of 0–1 × 10
4
cfu mL
−1
(
R
2
= 0.9934). Additionally, as the concentration of
V. parahaemolyticus
increases, the reaction solution changes from bluish green to green and then to yellow, enabling semi-quantitative detection by visual observation with a visual detection limit of 10 cfu mL
−1
. Furthermore, the developed dual-substrate colorimetric method demonstrates excellent selectivity for
V. parahaemolyticus
detection and satisfactory recovery rates when applied to the determination of
V. parahaemolyticus
in food samples.

IMPORTANCE

The Fe
3
O
4
-Zn-Mn nanomimetic enzyme demonstrates significant importance in dual-substrate colorimetric detection for
V. parahaemolyticus
, owing to its enhanced sensitivity, selectivity, and rapid detection capabilities. Additionally, it offers cost-effectiveness, portability, and the potential for multiplex detection. This innovative approach holds promise for improving the monitoring and control of
V. parahaemolyticus
infections, thereby contributing to advancements in public health and food safety.
Toxin/antitoxin systems induce persistence and work in concert with restriction/modification systems to inhibit phage
Authors: Fernández-García Laura , Song Sooyeon , Kirigo Joy , Battisti Michael E. , Petersen Maiken E. , Tomás María , Wood Thomas K. 
Published: December 2023

ABSTRACT

Myriad bacterial anti-phage systems have been described and often the mechanism of programmed cell death is invoked for phage inhibition. However, there is little evidence of “suicide” under physiological conditions for these systems. Instead of death to stop phage propagation, we show here that persister cells, i.e., transiently-tolerant, dormant, antibiotic-insensitive cells, are formed and survive using the
Escherichia coli
C496_10 tripartite toxin/antitoxin system MqsR/MqsA/MqsC to inhibit T2 phage. Specifically, MqsR/MqsA/MqsC inhibited T2 phage by 10
5
-fold and reduced T2 titers by 3,000-fold. During T2 phage attack, in the presence of MqsR/MqsA/MqsC, evidence of persistence includes the single-cell physiological change of reduced metabolism (via flow cytometry), increased spherical morphology (via transmission electron microscopy), and heterogeneous resuscitation. Critically, we found restriction-modification systems (primarily EcoK McrBC) work in concert with the toxin/antitoxin system to inactivate phage, likely while the cells are in the persister state. Hence, a phage attack invokes a stress response similar to antibiotics, starvation, and oxidation, which leads to persistence, and this dormant state likely allows restriction/modification systems to clear phage DNA.

IMPORTANCE
To date, there are no reports of phage infection-inducing persistence. Therefore, our results are important since we show for the first time that a phage-defense system, the MqsRAC toxin/antitoxin system, allows the host to survive infection by forming persister cells, rather than inducing cell suicide. Moreover, we demonstrate that the MqsRAC system works in concert with restriction/modification systems. These results imply that if phage therapy is to be successful, anti-persister compounds need to be administered along with phages.
Regional and temporal variations of spontaneous coronary artery dissection care according to consensus recommendations: a systematic review and meta-analysis
Authors: Dang Quan , Othman Farrah , Sheahen Brodie , Marschner Simone , Psaltis Peter , Al-Lamee Rasha Kadem , Szirt Richard , Chong James , Zaman Sarah 
Published: December 2023

AimThe first expert consensus documents on management of patients with spontaneous coronary artery dissection (SCAD) were published in 2018. Worldwide quality of care, as measured by adherence to these recommendations, has not been systematically reviewed. We aim to review the proportion of patients with SCAD receiving consensus recommendations globally, regionally and, determine differences in practice before and after 2018.Methods and resultsA systematic review was performed by searching four main databases (Medline, Embase, SCOPUS, CINAHL) from their inception to 16 June 2022. Studies were selected if they included patients with SCAD and reported at least one of the consensus document recommendations. 53 studies, n=8456 patients (mean 50.1 years, 90.6% female) were included. On random effects meta-analysis, 92.1% (95% CI 89.3 to 94.8) received at least one antiplatelet, 78.0% (CI 73.5 to 82.4) received beta-blockers, 58.7% (CI 52.3 to 65.1) received ACE inhibitors or aldosterone receptor blockers (ACEIs/ARBs), 54.4% (CI 45.4 to 63.5) were screened for fibromuscular dysplasia (FMD), and 70.2% (CI 60.8 to 79.5) were referred to cardiac rehabilitation. Except for cardiac rehabilitation referral and use of ACEIs/ARBs, there was significant heterogeneity in all other quality-of-care parameters, across geographical regions. No significant difference was observed in adherence to recommendations in studies published before and after 2018, except for lower cardiac rehabilitation referrals after 2018 (test of heterogeneity, p=0.012).ConclusionThere are significant variations globally in the management of patients with SCAD, particularly in FMD screening. Raising awareness about consensus recommendations and further prospective evidence about their effect on outcomes may help improve the quality of care for these patients.