bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of different pickling methods on physicochemical properties and flavor profiles of Tongling white ginger: Dry‐salting, brine‐pickling, and inoculation‐pickling
Authors: Zong Kaili , Jin Feixiang , Wang Daquan , Hu Hongchao , Cui Haipeng , Yang Jianting 
Published: January 2024

AbstractTongling white ginger is a Chinese fermented vegetable with unique flavors. However, little is known about its physicochemical properties, flavor characteristics, and sensory evaluation. The study examined the physicochemical (pH, titratable acidity [TA], nitrite, soluble protein, and color) and flavor characteristics (organic acids, free amino acids, and volatiles) of white ginger during fermentation. The results showed that the pH value and soluble protein in the dry‐salted, brine‐pickled, and inoculation‐pickled decreased significantly while the TA value increased significantly, inoculation‐pickled can effectively reduce the content of nitrite. After fermentation, inoculation‐pickled produced the highest content of organic acids, while dry‐salted produced the highest total amount of free amino acids. A total of 70, 68, 70, and 69 volatile compounds were identified in fresh, dry‐salted, brine‐pickled, and inoculation‐pickled white ginger. The total contents of terpenoids of Tongling white ginger by three fermentation methods decreased; the total contents of alcohols and aldehydes were the highest in brine‐pickled, and esters and ketones were more abundant in inoculation‐pickled. The results showed that inoculation‐pickled could shorten the fermentation time of Tongling white ginger, produce a unique flavor, and have the highest sensory score.
Evaluation of fig‐milk dessert bioactive properties as a potential functional food
Authors: Zare Niloofar , Sedighi Mahsa , Jalili Hasan , Zare Hamid , Maftoon Azad Neda 
Published: January 2024

AbstractThe fig‐milk dessert, a traditional and nutritionally rich treat infused with bioactive compounds, was subjected to a comprehensive analysis in this study. The novelty of this research lies in the investigation of the in vitro antioxidant, anticancer, and antimicrobial potential of the fig‐milk dessert. This was accomplished through the utilization of the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, Annexin/propidium iodide staining, microtiter plate‐based assay and agar well diffusion, respectively, for the first time. Additionally, the study assessed the total phenols and flavonoid content of the extract using the Folin–Ciocalteu assay and the aluminum chloride method, respectively. The findings revealed that the cooking method exerted a significant influence on the bioactive properties and nutritional composition of the dessert. Among the samples analyzed, CM1, consisting of figs steamed for 2 min and milk heated to 70°C, exhibited remarkable characteristics. This sample demonstrated the highest peptide concentration (1290 mg/L), superior antioxidant and anticancer activities, and favorable sensory attributes. Specifically, CM1 induced apoptosis in 84% of AGS cells and inhibited 68% of free radicals in the DPPH assay. It is noteworthy that the fig‐milk dessert did not exhibit any antibacterial properties. These discerning results carry substantial implications for the development of functional dairy products endowed with both nutritional and potential therapeutic properties.
Correlation of TIRADS scoring in thyroid nodules with preoperative fine needle aspiration biopsy and postoperative specimen pathology
Authors: Atar Cihan , Dalcı Kubilay , Aktar Yasemin , Totik Nazlı , Topal Uğur , Eray İsmail Cem , Akçam Atılgan Tolga , Sakman Gürhan 
Published: January 2024

AbstractIntroductionThe aim of our study is to determine the value of Thyroid Imaging Reporting and Data Systems (TIRADS) scoring in predicting malignancy in thyroid nodules by examining its relationship with fine needle aspiration biopsy and postoperative histopathological results.Materials and MethodsIn this study, patients who underwent surgery after ultrasonographic examination and fine needle aspiration biopsy for thyroid nodules at the General Surgery Clinic of Çukurova University Faculty of Medicine between January 2014 and November 2021 were retrospectively analyzed. The thyroid ultrasonography and fine needle aspiration biopsy of the included patients were performed by a clinician with 15 years of experience. The ultrasonographic features of the nodules were re‐evaluated by the same clinician, and the American College of Radiology (ACR) TIRADS score was determined. Fine needle aspiration biopsy results were grouped according to Bethesda criteria. Postoperative histopathological examination results were divided into two groups: benign and malignant. The ACR TIRADS score was compared with fine needle aspiration biopsy and histopathological results. The performance of the ACR TIRADS score in predicting malignancy was determined.Results79.8% of the 397 patients were female, and the mean age was 50.9 ± 12.8 years. The mean diameter of the nodules was 27.4 ± 15.8 mm. There was a significant, positive, but weak correlation between ACR TIRADS and Bethesda (p < 0.001) (r = 0.33). When the ACR TIRADS score was compared with histopathological results, it was found that the rate of malignancy increased as the TIRADS score increased (p < 0.001). The rates of malignancy diagnosis were 0% for TR1, 13.2% for TR2, 21.7% for TR3, 50.3% for TR4, and 72.4% for TR5. The area under the receiver operating characteristic curve for TIRADS in predicting malignancy was 0.747 (95% CI: 0.699–0.796, p < 0.001). TIRADS can distinguish malignancy with 75% accuracy. The optimal cutoff point was determined as TR4 with 80.3% sensitivity and 60.8% specificity.ConclusionThe ACR TIRADS scoring system is an effective risk classification system for thyroid nodules, providing 75% accuracy in predicting malignancy, with 80.3% sensitivity and 60.8% specificity values.
ALCAM is a biomarker of tumor aggressiveness and worse prognosis in glottic laryngeal squamous cell carcinoma
Authors: Nicolau‐Neto Pedro , Peryassú Bernardo Cacciari , de Carvalho Flávia Nascimento , Souza‐Santos Paulo Thiago , Valverde Priscila , Nascimento Cristina Moreira , Costa Izabella , Dias Fernando L. , Pinto Luis Felipe Ribeiro 
Published: January 2024

AbstractBackgroundLaryngeal squamous cell carcinoma (LSCC) is the second most frequent head and neck tumor. Prognosis of patients with LSCC has not improved in recent decades, showing a need for the identification of prognostic biomarkers and new therapeutic targets. Recently, we showed that ALCAM overexpression was associated with glottic LSCC prognosis.Objectives and methodsAiming to validate the prognostic value of ALCAM, we evaluate the ALCAM protein levels by immunohistochemistry in 263 glottic LSCC surgically treated with neck dissection.ResultsALCAM was expressed in 48.7% and overexpressed in 36.5% of glottic LSCC samples. ALCAM overexpression was associated with lymph node metastasis (p = 0.030), lymphovascular involvement (p = 0.0002), high‐grade tumors (p = 0.025), and tumor relapse (p = 0.043). Multivariate survival analyses showed an overfitting between ALCAM overexpression and lymph node metastasis as a prognostic variable.ConclusionsHigh ALCAM expression was associated with an aggressive glottic LSCC profile.
Streamflow change of major rivers over the Tibetan Plateau during the last half century and its possible causes
Authors: Yong Bin , Qi Weiqing , Lu Dekai , Lyu Yi , Liao Aiming , Wang Guoqing , Ren Liliang , Zhang Jianyun 
Published: January 2024

AbstractThe streamflow on the Tibetan Plateau (TP) plays an important role in the water supply of Asia's main river basins. To enhance understanding of hydrologic cycle under the pronounced warming over the TP, this study comprehensively investigates the streamflow changes at the upstream of six major rivers (Yellow River, Yalong River, Jinsha River, Lancang River, Nu River, and Yarlung Zangbo River) originating from the TP, and then diagnoses their possible causes by analysing the impacts of climate variability and human activities. Results indicate that these six major rivers studied have generally insignificant increasing trends in annual streamflow during the last half century, except for two stations. The significant increase appears at the Tuotuohe station in the headwater area of Jinsha River, while the dramatic decrease occurs at the Yunjinghong station in the downstream of Lancang River. In terms of climate factors, the six river basins show a distinct warming trend, along with a noticeable increase in precipitation over the central and northern regions. Pan evaporation, wind speed, sunshine duration, and relative humidity have been found to gradually decrease in most areas. As for the Tuotuohe station, both warming‐induced meltwater and increasing precipitation might jointly contribute to the increasing streamflow. But for the Yunjinghong station, the results simulated by the Variable Infiltration Capacity (VIC) model indicate that human activities, especially for the impoundment processes of Xiaowan and Nuozhadu dams, significantly influenced the streamflow, contributing to approximately 69% of the streamflow reduction during 2009–2013. In the context of accelerated global warming, greater attention should be paid to hydrometeorological changes on the TP to offer further insights for the water resources management of the ‘Asian Water Tower’.
Stable isotopic compositions and controlling factors of precipitation and atmospheric vapour in the headwaters of the Shule River
Authors: Zhang Zihan , Zhao Liangju , Wang Ninglian , Liu Hang , Xie Cong , Dong Xiying , Li Xingru 
Published: January 2024

AbstractStable isotopic compositions of precipitation (δ18Op, δ2Hp and d‐excessp) and atmospheric vapour (δ18Ov, δ2Hv and d‐excessv) with high spatial–temporal resolution are crucial in revealing hydrologic cycle. Based on the variation characteristics of δ18Op/δ18Ov, δ2Hp/δ2Hv and d‐excessp/d‐excessv in the headwaters of the Shule River (HSR) on hourly and daily scales from June to September 2018, this study analysed the relationships between δ18Op/δ2Hp and δ18Ov/δ2Hv combined with the equilibrium fractionation model, as well as δ18Op/δ18Ov and meteorological factors. The slopes of local meteoric water line (LMWL) and the δ2Hv‐δ18Ov fitting equation were similar (7.96 and 7.94) with both intercepts exceeding 10, reflecting the great contribution of recycling moisture. The values of δ18Ov/δ2Hv were lower than δ18Op/δ2Hp but with consistent variation patterns throughout the period. The equilibrium simulation results suggested that precipitation and atmospheric vapour almost approached isotopic equilibrium state, especially during monsoon intrusion period. Affected by monsoon intrusion, the slopes and intercepts of the LMWLs and the δ2Hv‐δ18Ov fitting equations were smaller than those during non‐monsoon period and d‐excess and δ18O were negatively correlated. Relative humidity had significant negative correlations with δ18Op and δ18Ov in the whole period, however, the positive correlations between δ18Op/δ18Ov and temperature were observed during non‐monsoon and monsoon intrusion period, respectively. Our results demonstrated that precipitation and atmospheric vapour isotopic compositions exhibited consistency under the influence of diverse moisture sources, while more complex relationships were found between δ18Op/δ18Ov and meteorological factors. This research provided evidence for using the isotopic compositions of atmospheric vapour to indicate moisture sources, and can improve understanding of the water cycle and eco‐hydrological process from the perspective of the interaction between water and gas phases of the inland river basin in northwest China.
Ocean air masses from the East Asian monsoon dominate the land‐surface atmospheric water cycles in the coastal areas of Liaodong Bay, Northeast China
Authors: Du Yi‐Yuan , Wen Ri‐Hong , Jia Qing‐Yu , Liu Lu‐Lu , Hu Yue‐Hua , Li Jia‐Yuan , Pan Jie , Yuan Xia , Peng Jiang , Zakari Sissou , Panthee Shristee , Song Liang , Liu Wen‐Jie , Yang Bin 
Published: January 2024

AbstractLong‐term atmospheric water vapour hydrogen (δ2H), oxygen (δ18O) and deuterium excess (d‐excess) can provide unique insights into the land‐atmosphere coupling processes. The in‐situ measurements of atmospheric water vapour δ2H, δ18O and d‐excess were conducted above a reed wetland of Liaodong Bay (2019–2020). We found significant inter‐annual variations in atmospheric water vapour isotopes between the two growing (May–September) seasons. The δ2H, δ18O and d‐excess of atmospheric water vapour exhibited different seasonal and diurnal cycles concerning the vertical measurement heights, especially in 2019. The isotopic differences of atmospheric water vapour among vertical measurement heights were more evident in the daytime. Rainfall events directly impacted the diurnal patterns of water vapour isotopes, and the influences depended on rainfall intensities. However, only weak correlations existed between water vapour isotopes and local meteorological factors (R2 = 0.01–0.16, p < 0.001), such as water vapour concentration (w), Relative Humidity (RH) and surface air temperature (Ta). Based on the back‐air trajectory analyses, the spatial–temporal dynamics of atmospheric water vapour isotopes are highly synchronized with monsoon activities. Different water vapour sources influence the water vapour isotope in this region and the higher d‐excess value is related to the intense convection brought by the monsoon. High‐resolution measurements of atmospheric water vapour isotopes will improve our understanding of the hydrological cycles in coastal areas.
Runoff response to multiple land‐use changes and climate perturbations
Authors: Yang Mingjie , Xue Lianqing , Liu Yuanhong , Wang Wenzhuang , Han Qiang , Liu Saihua , Fu Ruizhe 
Published: January 2024

AbstractRunoff response to multiple land‐use changes and climate perturbations is distinct, and the main influencing factors vary significantly in different regions. However, few have simultaneously considered the effects of multiple land use changes (vegetation cover changes, terraces and check‐dams construction, and urban expansion) and climatic perturbations (precipitation, potential evapotranspiration, and temperature) on runoff and constructed separate expressions for distinct study areas. This article attempted to determine the main influencing factors of runoff according to the fitting function in the eight subregions of the middle Yellow River (MYR), construct the expressions between the controlling parameter in the Budyko framework and the main factors, and quantify the contribution of climate factors and land use changes to runoff by combining the elasticity coefficient in each subregion. The results indicated that climate factors and land use changes could significantly impact controlling parameters, and there were differences between regions. Climate change promoted an increase in runoff, while land use change promoted its reduction, and the reduction value outweighed its increase. In terms of land use changes, increasing vegetation coverage could suppress runoff reduction, while constructing terraces and check‐dams could promote its reduction. The urban expansion benefited runoff collection and, therefore, could suppress runoff reduction. Its absolute contribution rate exceeded 200% in apparent urban expansion areas. In addition, the contribution rates of land use to runoff changes in the northern arid and semi‐arid regions were significantly higher than those in the southern, and they were more sensitive to land use changes. The research results can provide a reference for analysing the runoff response to different land‐use changes and can further advance people's understanding of the water cycle.
Augmented drug resistance of osteosarcoma cells within decalcified bone matrix scaffold: The role of glutamine metabolism
Authors: Ren Jiaxin , Zhao Cheng , Sun Ruizhu , Sun Jian , Lu Laiya , Wu Jun , Li Shuaijun , Cui Lei 
Published: January 2024

AbstractDue to the lack of a precise in vitro model that can mimic the nature microenvironment in osteosarcoma, the understanding of its resistance to chemical drugs remains limited. Here, we report a novel three‐dimensional model of osteosarcoma constructed by seeding tumor cells (MG‐63 and MNNG/HOS Cl no. 5) within demineralized bone matrix scaffolds. Demineralized bone matrix scaffolds retain the original components of the natural bone matrix (hydroxyapatite and collagen type I), and possess good biocompatibility allowing osteosarcoma cells to proliferate and aggregate into clusters within the pores. Growing within the scaffold conferred elevated resistance to doxorubicin on MG‐63 and MNNG/HOS Cl no. 5 cell lines as compared to two‐dimensional cultures. Transcriptomic analysis showed an increased enrichment for drug resistance genes along with enhanced glutamine metabolism in osteosarcoma cells in demineralized bone matrix scaffolds. Inhibition of glutamine metabolism resulted in a decrease in drug resistance of osteosarcoma, which could be restored by α‐ketoglutarate supplementation. Overall, our study suggests that microenvironmental cues in demineralized bone matrix scaffolds can enhance osteosarcoma drug responses and that targeting glutamine metabolism may be a strategy for treating osteosarcoma drug resistance.
Tuning the Sign and Magnitude of Complexation‐Induced pKa Shifts in Cucurbit[7]uril Host‐Guest Complexes by Molecular Engineering
Authors: Trevisan Alberto , Ferreira Ana S. D. , Marrec Philippe , Parola A. Jorge , Basílio Nuno 
Published: January 2024

AbstractCucurbiturils are popular macrocyclic receptors that bind complementary guest molecules with high affinity in aqueous environments. They are recognized for their ability to selectively bind positively charged guest molecules, including ionizable ammonium cations which frequently display much higher affinity than their neutral counterparts. This selectivity for the protonated species is translated into an increase in the basicity of encapsulated guests (i. e. into complexation‐induced positive pKa shifts). However, despite being very rare, negative pKa shifts can be observed for specific guests. Following a previous work from our group reporting slightly negative pKa shifts for flavylium and chalcone dyes featuring N‐diethylamino substituents (ΔpKa=− 0.2), herein we report a systematic study on the complexation of N‐dialkylaminochalcones with CB7. The results show that the pKa shifts of these host‐guest complexes can be rationally tuned by the nature of the N‐dialkylamino groups and as well by target substitutions on the skeleton of the dye, allowing the design of a CB7 1 : 1 host‐guest complex with a ΔpKa=− 0.6.
Does institutional quality matter for renewable energy promotion in OECD economies?
Authors: Rafiq Shuddhasattwa , Paramati Sudharshan Reddy , Alam Md. Samsul , Hafeez Khalid , Shafiullah Muhammad 
Published: January 2024

AbstractThis study examines the effect of institutional quality on renewable energy promotion in OECD economies. The study employs annual data from 1980 to 2014 on 18 OECD economies. The robust panel unit root tests show that all the considered variables have a similar order of integration, indicating that they are nonstationary at their levels but stationary at the first‐order differences. The panel cointegration test with structural breaks and cross‐section dependence confirms a long‐run equilibrium association between institutional quality, renewable energy consumption and control variables. The analysis of long‐run estimations displays that better institutional quality makes a unique and substantial contribution to promoting renewable energy consumption. Overall, the study findings offer important policy implications highlighting the importance of institutional quality for the growth of renewable energy and a sustainable world.
Social support buffers the impact of pregnancy stress on perceptions of parent–infant closeness during the COVID‐19 pandemic
Authors: Becker Emma , Atkinson Leslie , Gonzalez Andrea , Khoury Jennifer 
Published: January 2024

AbstractPregnant individuals and parents have experienced elevated mental health problems and stress during COVID‐19. Stress during pregnancy can be harmful to the fetus and detrimental to the parent–child relationship. However, social support is known to act as a protective factor, buffering against the adverse effects of stress. The present study examined whether (1) prenatal stress during COVID‐19 was associated with parent–infant closeness at 6 months postpartum, and (2) social support moderated the effect of prenatal stress on the parent–infant relationship. In total, 181 participants completed questionnaires during pregnancy and at 6 months postpartum. A hierarchical linear regression analysis was conducted to assess whether social support moderated the effect of stress during pregnancy on parent–infant closeness at 6 months postpartum. Results indicated a significant interaction between prenatal stress and social support on parents’ perceptions of closeness with their infants at 6 months postpartum (β = .805, p = .029); parents who experienced high prenatal stress with high social support reported greater parent–infant closeness, compared to those who reported high levels of stress and low social support. Findings underscore the importance of social support in protecting the parent–infant relationship, particularly in times of high stress, such as during the COVID‐19 pandemic.
Leveling the field: Development of an asynchronous interactive module series for genetic counseling trainees on molecular testing and variant interpretation
Authors: Reichert Sara L. , Dechene Elizabeth , Lulis Lauren , Valverde Kathleen , Conway Laura , Dulik Matthew 
Published: January 2024

AbstractThe need for education focusing on genomic technologies and variant interpretation for genetic counseling trainees has prompted genetic counseling training programs to alter their curricula to incorporate this content. Given students' diverse experiences prior to matriculation into genetic counseling training programs, students' familiarity with these topics may vary. Following receipt of feedback from trainees at a large genetic counseling program regarding an existing course focused on molecular technologies, a three‐part asynchronous module series was created as a prerequisite to this course as an opportunity to align knowledge. Designed to be completed by students on their own time and at their own pace, the modules allowed for additional instruction without increases in classroom time or credit hours. Content included a refresh on genetics concepts and an introduction to available genetics resources for developing a differential diagnosis as well as variant interpretation framework. Modules utilized a clinical scenario to anchor learning with interactive content, allowing students to progress at their own pace and explore content as they found necessary. Completion of this asynchronous module series was required by incoming first‐year students prior to the start of the academic semester. Following completion, students were asked to provide feedback on the module series. Reviews were primarily positive with students indicating that while the content was not entirely new, they found the review valuable and would be likely to reference the modules later in their genetic counseling training. Areas identified for improvement included additional detail regarding genetic testing methods as well as adjusting the interactive content to ensure accessibility for all students and systems. Taken together, the development and implementation of this asynchronous series as an additional component to genetic counseling training was considered a success and this approach can be considered to address additional topics dependent on a programs' needs.