bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell RNA sequencing analysis identifies one subpopulation of endothelial cells that proliferates and another that undergoes the endothelial-mesenchymal transition in regenerating pig hearts
Authors: Nguyen Thanh Minh , Geng Xiaoxiao , Wei Yuhua , Ye Lei , Garry Daniel J. , Zhang Jianyi 
Published: January 2024

Background: In our previous work, we demonstrated that when newborn pigs undergo apical resection (AR) on postnatal day 1 (P1), the animals’ hearts were completely recover from a myocardial infarction (MI) that occurs on postnatal day 28 (P28); single-nucleus RNA sequencing (snRNAseq) data suggested that this recovery was achieved by regeneration of pig cardiomyocyte subpopulations in response to MI. However, coronary vasculature also has a key role in promoting cardiac repair.Method: Thus, in this report, we used autoencoder algorithms to analyze snRNAseq data from endothelial cells (ECs) in the hearts of the same animals.Main results: Our results identified five EC clusters, three composed of vascular ECs (VEC1-3) and two containing lymphatic ECs (LEC1-2). Cells from VEC1 expressed elevated levels of each of five cell-cyclespecific markers (Aurora Kinase B [AURKB], Marker of Proliferation Ki-67 [MKI67], Inner Centromere Protein [INCENP], Survivin [BIRC5], and Borealin [CDCA8]), as well as a number of transcription factors that promote EC proliferation, while (VEC3 was enriched for genes that regulate intercellular junctions, participate in transforming growth factor β (TGFβ), bone morphogenic protein (BMP) signaling, and promote the endothelial mesenchymal transition (EndMT). The remaining VEC2 did not appear to participate directly in the angiogenic response to MI, but trajectory analyses indicated that it may serve as a reservoir for the generation of VEC1 and VEC3 ECs in response to MI. Notably, only the VEC3 cluster was more populous in regenerating (i.e., ARP1MIP28) than non-regenerating (i.e., MIP28) hearts during the 1-week period after MI induction, which suggests that further investigation of the VEC3 cluster could identify new targets for improving myocardial recovery after MI. Histological analysis of KI67 and EndMT marker PDGFRA demonstrated that while the expression of proliferation of endothelial cells was not significantly different, expression of EndMT markers was significantly higher among endothelial cells of ARP1MIP28 hearts compared to MIP28 hearts, which were consistent with snRNAseq analysis of clusters VEC1 and VEC3. Furthermore, upregulated secrete genes by VEC3 may promote cardiomyocyte proliferation via the Pi3k-Akt and ERBB signaling pathways, which directly contribute to cardiac muscle regeneration.Conclusion: In regenerative heart, endothelial cells may express EndMT markers, and this process could contribute to regeneration via a endothelial-cardiomyocyte crosstalk that supports cardiomyocyte proliferation.
Intermediate and long-term residual cardiovascular risk in patients with established cardiovascular disease treated with statins
Authors: Vijayaraghavan K. , Baum S. , Desai N. R. , Voyce S. J. 
Published: January 2024

IntroductionStatins remain the first-line treatment for secondary prevention of cardiovascular (CV) events, with lowering of low-density lipoprotein cholesterol (LDL-C) being their therapeutic target. Although LDL-C reduction significantly lowers CV risk, residual risk persists, even in patients with well-controlled LDL-C; thus, statin add-on agents that target pathways other than LDL-C, such as the omega-3 fatty acid eicosapentaenoic acid, may help to further reduce persistent CV risk in patients with established CV disease.MethodsThis narrative review examines the contemporary literature assessing intermediate- and long-term event rates in patients with established CV disease treated with statins.ResultsCV event rates among patients treated with statins who have established CV disease, including coronary artery disease, cerebrovascular disease, or peripheral arterial disease, accumulate over time, with a cumulative incidence of CV events reaching up to approximately 40% over 10 years. Recurrent stroke occurs in up to 19% of patients seven years after a first cerebrovascular event. Repeat revascularization and CV-related death occurs in up to 38% and 33% of patients with peripheral artery disease after three years, respectively.DiscussionAdditional treatment strategies, such as eicosapentaenoic acid, are needed to reduce persistent CV risk in patients with established CV disease treated with statins.
Implantation of atrial flow regulator devices in patients with congenital heart disease and children with severe pulmonary hypertension or cardiomyopathy—an international multicenter case series
Authors: Butera Gianfranco , Piccinelli Enrico , Kolesnik Adam , Averin Konstantin , Seaman Cameron , Castaldi Biagio , Cuppini Elena , Fraisse Alain , Bautista-Rodriguez Carles , Hascoet Sebastien , D'Amore Carmen , Baruteau Alban-Elouen , Blasco Pedro Betrián , Bianco Lisa , Eicken Andreas , Jones Matthew , Kuo James A. , Rajszys Grazyna Brzezinska 
Published: January 2024

BackgroundThe Occlutech Atrial Flow Regulator (AFR) is a self-expandable double-disc nitinol device with a central fenestration. Its use has been approved in the adult population with heart failure and described for pulmonary hypertension (PH). Only case reports and small series have been published about its use in the paediatric population and for congenital heart disease (CHD).ObjectivesThe authors sought to investigate the feasibility, safety, and short-term follow-up of AFR implantation in patients with CHD or children with PH or cardiomyopathy.MethodsThis is a multicenter retrospective study involving 10 centers worldwide. Patients of any age with CHD or patients aged &lt; 18 years with PH or cardiomyopathy needing AFR implantation were included.ResultsA total of 40 patients underwent AFR implantation. The median age of the population at the time of the procedure was 58.5 months (IQR: 31.5–142.5) and the median weight was 17 kg (IQR: 10–46). A total of 26 (65.0%) patients had CHD, nine (22.5%) children, a cardiomyopathy, and five (12.5%), a structurally normal heart. The implantation success rate was 100%. There were two early and one late device thrombosis. Two patients (5.0%) with dilated cardiomyopathy on extracorporeal membrane oxygenator (ECMO) died during the hospital stay. At a median follow-up of 330 days (IQR: 125–593), 37 (92.5%) patients were alive. At follow-up, 20 patients improved their New York Heart Association (NYHA) class, 12 patients did not change their NYHA class, and one patient with idiopathic PH worsened.ConclusionsAFR implantation in patients with CHD and children with severe PH or cardiomyopathy is promising and seems to have beneficial effects at short-term follow-up.
Clinical implications of respiratory ciliary dysfunction in heterotaxy patients with congenital heart disease: elevated risk of postoperative airway complications
Authors: Zhao Tingting , Huang Xianghui , Chen Weicheng , Gao Han , Feng Zhiyu , Tan Chaozhong , Sun Jingwei , Ma Xiaojing , Yan Weili , Sheng Wei , Huang Guoying 
Published: January 2024

ObjectiveCardiac surgery in Congenital Heart Disease-Heterotaxy (CHD-HTX) patients often leads to increased postoperative airway complications. Abnormal respiratory ciliary function, resembling primary ciliary dyskinesia, has been observed. We expanded the sample size by retrospectively reviewing Ciliary Dysfunction (CD) in CHD-HTX patients to verify the increased risk of post-surgical respiratory complications.MethodsWe conducted a retrospective review of 69 CHD-HTX patients undergoing cardiac surgery, assessing abnormal respiratory function using nasal nitric oxide (nNO) levels and nasal ciliary motion observed in video microscopy. Data collected included demographics, surgical details, postoperative complications, length of stay, ICU hours, salvage procedures, intubation duration, and mortality.ResultsThe CD and no-CD cohorts exhibited notable similarities in risk adjustment in Congenital Heart Surgery-1 (RACHS-1) risk categories, age at the time of surgery, and the duration of follow-up evaluations. We observed a trend toward an increased length of post-operative stay in the CD group (15.0 vs. 14.0; P = 0.0017). CHD-HTX patients with CD showed significantly higher rates of respiratory complications (70% vs. 44.4%; P = 0.008). There were no notable variances observed in postoperative hospitalization duration, mechanical ventilation period, or surgical mortality.ConclusionOur findings suggest that CHD-HTX patients with CD may face an elevated risk of respiratory complications. These results offer guidance for perioperative management and serve as a reference for further pathological studies.
Case report: Twice-daily tolvaptan dosing regimen in a challenging case of hyponatremia due to SIAD
Authors: Arecco Anna , Demontis Davide , Della Sala Leonardo , Musso Natale , Gay Stefano , Boschetti Mara , Ferone Diego , Gatto Federico 
Published: January 2024

BackgroundSyndrome of inappropriate antidiuresis (SIAD) is one of the most frequent causes of euvolemic hyponatremia (serum sodium levels &lt; 135 mEq/L) and it represents more than 35% of hyponatremia cases in hospitalized patients. It is characterized by an inappropriate vasopressin (AVP)/antidiuretic hormone (ADH) secretion, which occurs independently from effective serum osmolality or circulating volume, leading to water retention via its action on type 2 vasopressin receptor in the distal renal tubules. Corpus callosum agenesis (CCA) is one of the most common congenital brain defects, which can be associated to alterations in serum sodium levels. This report presents a rare case of chronic hyponatremia associated with SIAD in a woman with CCA, whose correction of serum sodium levels only occurred following twice-daily tolvaptan administration.Case presentationA 30-year-old female was admitted to our hospital for non-acute hyponatremia with dizziness, headache, distal tremors, and concentration deficits. She had profound hyponatremia (Na 121 mmol/L) with measured plasma hypo-osmolality (259 mOsm/Kg) and urinary osmolality greater than 100 mOsm/Kg (517 mOsm/Kg). She presented clinically as normovolemic. After the exclusion of other causes of normovolemic hyponatremia, such as hypothyroidism and adrenal insufficiency, a diagnosis of SIAD was established. We have ruled out paraneoplastic, inflammatory, and infectious causes, as well as ischemic events. Her medical history showed a CCA and frontal teratoma. We administered tolvaptan initially at a low dosage (15 mg once a day) with persistence of hyponatremia. Therefore, the dosage was first doubled (30 mg once a day) and then increased to 45 mg once a day with an initial improvement in serum sodium levels, although not long-lasting. We therefore tried dividing the 45 mg tolvaptan administration into two doses of 30 mg and 15 mg respectively, using an off-label treatment schedule, thus achieving long-lasting serum sodium levels in the low-normal range associated with a general clinical improvement.ConclusionsThis report underlines the importance of the correct diagnosis, management and treatment of SIAD, as well as the need for further studies about the pharmacokinetics and pharmacodynamics of vasopressin receptor antagonists.
Construction and application of workover priority evaluation model for surface coalbed methane low production wells based on Entropy-TOPSIS
Authors: Li Yanhe , Zhu Lin , Lv Runsheng , Ni Xiaoming 
Published: January 2024

Affected by geology and mining, a certain number of low-yield wells will appear after a period of production in coalbed methane wells, and technical repairs are urgently needed to restore their production capacity to the maximum. Therefore, it is of great significance to establish a reasonable and feasible theoretical standard to evaluate the workover priority of CBM wells for the efficient utilization of CBM resources. From the perspectives of technical feasibility and economic rationality, eight key indicators that affect the cost performance of CBM well workover are screened. Combining the entropy value method and the TOPSIS comprehensive ranking method, the surface coalbed methane well repair priority entropy value-TOPSIS comprehensive evaluation model was constructed, and the surface coalbed methane well repair priority evaluation was carried out by taking 9 surface coalbed methane wells that urgently needed to be repaired in a mine as the evaluation objects. The evaluation results show that among the economic indicators, the average gas production per meter in the 30 days before the coal bed methane well was stopped and the cumulative gas production per meter before the stop had a relatively high weight, which were 29.68% and 13.83% respectively, This shows that in coalbed methane well workover operations, the long-term gas production potential of the coalbed methane well and the gas production capacity when disturbed by harmful factors are the decisive factors affecting the priority of coalbed methane well workover; Among the technical indicators, the degree of casing deformation and the depth of the coalbed methane well have a relatively high weight, accounting for 11.34% and 9.90% respectively, This shows that the smaller the deformation of the wellbore casing and the shallower the depth of the coalbed methane well, the higher the priority of the workover of the coalbed methane well; The ranking of the production recovery rates of the three coalbed methane wells in the Pingdingshan mining area after workover operations is consistent with the results output by the evaluation model, which proves that the evaluation model is reasonable for determining the priority of workovers for damaged wellbores; This evaluation model can provide a theoretical reference for small and medium-sized coalbed methane development companies to determine the priority of workovers.
Satellite-based drought assessment in the endorheic basin of Lake Balkhash
Authors: Yegizbayeva Asset , Koshim Asyma G. , Bekmuhamedov Nurlan , Aliaskarov Duman T. , Alimzhanova Nazira , Aitekeyeva Nurgul 
Published: January 2024

Introduction: This study investigates into the drought-prone region of the Lake Balkhash basin, conducting a thorough analysis spanning 2 decades, emphasizing its significant impact on agriculture and water challenges in Central Asia. Beyond the specific focus on this region, the research aims to contribute valuable insights that extend our understanding of the broader implications of drought in the area.Methods: Utilizing MODIS satellite imagery, the study employs the Vegetation Health Index a comprehensive indicator combining the Vegetation Condition Index and Temperature Condition Index ranging from 0 (extreme aridity) to 100 (optimal moisture). The assessment of drought conditions from 2000 to 2020 includes probabilistic mapping, trend analysis, and Pearson’s correlation coefficients. Connections with hydrological factors, such as river water levels and the Balkhash Lake area, are explored, along with in-depth analyses of land use data and the GRACE dataset on water equivalent thickness, enhancing the study’s depth and reliability.Results: Drought affected approximately 44% of the Balkhash Lake Basin during the study period, impacting both Kazakhstan and China. Severe drought episodes occurred in 2000, 2008, 2014, and 2015, highlighting the region’s vulnerability. Analysis of drought trends revealed diverse patterns: 23% exhibited an increase, 17% showed a decrease, and 60% remained stable. Correlations between drought and hydrological parameters varied among stations, with positive correlations at Kapshagay and Shelek Stations, a weak correlation at Ayagoz Station, and a significant positive correlation at Lepsy despite the elevation.Discussion: This research underscores the intricate link between drought and hydrological factors in the Balkhash Lake Basin, emphasizing the need for precise water resource management and climate adaptation. Crucial strategies include proactive monitoring, tailored interventions, and the application of probabilistic drought mapping to enhance water supply management, contributing actionable insights for sustainable practices in the region.Conclusion: This study significantly advances our understanding of drought dynamics in the Balkhash Lake Basin, recommending adaptive strategies, site-specific interventions, and sustainable water management. The findings provide a crucial foundation for informed water resource decisions in Central Asia, emphasizing the importance of region-specific approaches to address diverse challenges posed by drought.
Characterizing bioaerosols in PM2.5 in a semi-arid region experiencing desert dust events
Authors: Karimi Hossein , Nikaeen Mahnaz , Shamsizadeh Zahra , Hajizadeh Yaghoub 
Published: January 2024

Introduction: Fine particulate matter (PM2.5) pollution is a global public health concern, particularly in arid and semi-arid regions that experience dust events. Exposure to PM2.5 has been associated with various short- and long-term health effects, which can be attributed to the composition of the fine particles. This study aimed to assess the concentration of bacteria and fungi in PM2.5 in the air of Isfahan, Iran which experiences air pollution.Methods: A total of thirty-nine PM2.5 samples were collected over 1 year. Bacterial and fungal concentrations associated with PM2.5 were measured using both culture-based method and real-time polymerase chain reaction (PCR).Results: The average concentration of PM2.5 was found to be 56 μg m−3, with many samples exceeding the recommended limit of 15 μg m−3 set by the World Health Organization for PM2.5 in ambient air. The mean culturable bacterial and fungal concentrations were 39 and 4 CFU m−3, respectively which showed a significant difference compared to the results obtained through real-time PCR. No significant correlation was found between the levels of bacterial and fungal cells and PM2.5 concentrations. The dominant phyla found in PM2.5 were Firmicutes and Actinobacteria, while Proteobacteria as Gram-negative bacteria were rarely detected.Discussion: The findings suggest that the climate conditions in Isfahan, characterized by its semi-arid nature with low humidity content, influences the activity and viability of microbial populations, resulting in low concentrations of bioaerosols in PM2.5. Furthermore, it seems that desert dust events do not contribute to increasing the potential health risks associated with bacterial and fungal bioaerosols in PM2.5.
Diadromous fish run assessment: a double-observer model using acoustic cameras to correct imperfect detection and improve population abundance estimates
Authors: Boulenger Clarisse , Roussel Jean-Marc , Beaulaton Laurent , Martignac François , Nevoux Marie 
Published: January 2024

IntroductionDiadromous fish populations have strongly declined over decades, and many species are protected through national and international regulations. They account for less than 1% of fish biodiversity worldwide, but they are among the most perceptible linkages between freshwater and marine ecosystems. During their migration back and forth, diadromous fish species are subjected to many anthropogenic threats, among which river damming can severely limit access to vital freshwater habitats and jeopardize population sustainability. Here, we developed a method based on a double-observer modeling approach for estimating the abundance of diadromous fish during their migration in rivers.MethodsThe method relies on two independent and synchronous records of fish counts that were analyzed jointly thanks to a hierarchical Bayesian model to estimate detection efficiencies and daily fish passage. We used simulated data to test model robustness and identify conditions under which the developed approach can be used. The approach was then applied to empirical data to estimate the annual silver eel run in the Touques River, France.ResultsThe analysis of simulated datasets and the study case gives evidence that the model can provide robust,accurate, and precise estimates of detection probabilities and total fish abundance in a set of conditions dependent on the information provided in the data (annual distribution of fish passage, annual number of observation, pairing period, etc.).DiscussionThen, the method can be applied to various species and counting systems, including nomad acoustic camera devices. We discuss its relevance for programs on river continuity restoration, notably to quantify population restoration associated with dam removals.
Gut microbiota does not play a mediating role in the causal association between inflammatory bowel disease and several its associated extraintestinal manifestations: a Mendelian randomization study
Authors: Lu Weipeng , Cen Jiepeng , Dai Qijie , Tao Heqing , Peng Liang 
Published: January 2024

Background and objectivesObservational study has found inflammatory bowel disease to be associated with multiple extraintestinal manifestations. To this end, we characterized the causal association between inflammatory bowel disease and extraintestinal manifestations through a Mendelian randomization study and further explored the role of intestinal flora in inflammatory bowel disease and the extraintestinal manifestations associated with it.Materials and methodsWe genetically predicted the causal relationship between inflammatory bowel disease and twenty IBD-related extraintestinal manifestations (including sarcoidosis, iridocyclitis, interstitial lung disease, atopic dermatitis, ankylosing spondylitis, psoriatic arthropathies, primary sclerosing cholangitis, primary biliary cholangitis). We used the full genome-wide association study (GWAS) summary statistics on gut microbiota in 18,340 participants from 24 cohorts to explore its role in the casual relationships between IBD and IBD-related extraintestinal manifestations. Inverse variance weighting (IVW) was used as the main analytical method to assess the causal associations. We performed Cochran’s Q test to examine the heterogeneity. To assess the robustness of the IVW results, we further performed sensitivity analyses including the weighted median method, MR-Egger regression, and Mendelian Randomization Pleiotropy RESidual Sum and Outlier (MR-PRESSO) test. The leave-one-out sensitivity analysis was further performed to monitor if significant associations were dominated by a single nucleotide polymorphism (SNP).ResultA total of eight extraintestinal manifestations were found to be at elevated risk of development due to inflammatory bowel diseases. A total of 11 causal relationships were found between IBD and gut microbiota, four of which were stable. Between gut microbiota and these eight extraintestinal manifestations, a total of 67 nominal causal associations were identified, of which 13 associations were stable, and notably 4 associations were strongly correlated.ConclusionThrough the two-sample MR analysis, we identified extraintestinal manifestations that were causally associated with inflammatory bowel disease and obtained multiple associations from inflammatory bowel disease and gut microbiota, and gut microbiota and extraintestinal manifestations in further analyses. These associations may provide useful biomarkers and potential targets for pathogenesis and treatment.