bluuebunny/crossref_metadata_2025_split huggingface dataset:
Absence of the primary cilia formation gene Talpid3 impairs muscle stem cell function
Authors: Martinez-Heredia Victor , Blackwell Danielle , Sebastian Sujith , Pearson Timothy , Mok Gi Fay , Mincarelli Laura , Utting Charlotte , Folkes Leighton , Poeschl Ernst , Macaulay Iain , Mayer Ulrike , Münsterberg Andrea 
Published: November 2023

AbstractSkeletal muscle stem cells (MuSC) are crucial for tissue homoeostasis and repair after injury. Following activation, they proliferate to generate differentiating myoblasts. A proportion of cells self-renew, re-enter the MuSC niche under the basal lamina outside the myofiber and become quiescent. Quiescent MuSC have a primary cilium, which is disassembled upon cell cycle entry. Ex vivo experiments suggest cilia are important for MuSC self-renewal, however, their requirement for muscle regeneration in vivo remains poorly understood. Talpid3 (TA3) is essential for primary cilia formation and Hedgehog (Hh) signalling. Here we use tamoxifen-inducible conditional deletion of TA3 in MuSC (iSC-KO) and show that regeneration is impaired in response to cytotoxic injury. Depletion of MuSC after regeneration suggests impaired self-renewal, also consistent with an exacerbated phenotype in TA3iSC-KO mice after repeat injury. Single cell transcriptomics of MuSC progeny isolated from myofibers identifies components of several signalling pathways, which are deregulated in absence of TA3, including Hh and Wnt. Pharmacological activation of Wnt restores muscle regeneration, while purmorphamine, an activator of the Smoothened (Smo) co-receptor in the Hh pathway, has no effect. Together, our data show that TA3 and primary cilia are important for MuSC self-renewal and pharmacological treatment can efficiently restore muscle regeneration.
Projecting spatial interactions between global population and land use changes in the 21st century
Authors: Yang Di , Luan Wei-Xin , Zhang Xiaoling 
Published: November 2023

AbstractPrevious research on global urban land expansion has primarily focused on accommodating the growing population without adequately considering the trade-offs between urban land and ecological services. Consequently, it is important to investigate the effect of this expansion on the ecological service system, while also examining the potential trade-offs between population growth and available urban land. Here, we quantify how different global land expansion options contribute to reconciling global population changes and to compliance with the UN Sustainable Development Goal 11 on sustainable cities and human settlements. Using an integrated framework for projecting global land consumption with the trade-off strategy between global land expansion and population under shared socioeconomic pathways (SSPs), we aim to bridge the knowledge gap in this area. The results of our study suggest that implementing a trade-off strategy between global land expansion and population growth can successfully reduce the levels of global land consumption in the future. Under the SSP1 scenario, for instance, our findings indicate that implementing trade-off policies can significantly reduce land consumption while increasing carbon sinks and protecting the global ecological service system. Specifically, converting land to forest or grassland can help decrease land consumption by 8.07% by the end of the 21st century. These insights can be valuable in designing land use policies that incorporate data-based solutions and address the challenges of sustainable urban development while also accommodating population growth.
Asymmetric seafloor depth across the Juan de Fuca Ridge caused by lithospheric heating
Authors: Wu Mengyu , Ritzwoller Michael H. 
Published: November 2023

AbstractPrevious studies attribute asymmetries across the East Pacific Rise to horizontal temperature or pressure gradients in the deep asthenosphere caused by the Pacific Superswell, which, however, cannot explain asymmetries observed across the Juan de Fuca Ridge. Here, we provide seismic evidence that the asymmetric seafloor depth across the Juan de Fuca Ridge is primarily caused by thermal buoyancy due to lithospheric heating and thinning. Based on a seismic model generated from Rayleigh wave measurements, we demonstrate that the seafloor depth on the western flank of the ridge, which is shallower ( > 150 m) than the prediction from the plate age, agrees with the relatively younger apparent thermal age inferred from the seismic data, whereas the buoyancy of the deeper asthenosphere alone can only account for <25% of the rise. On the eastern flank, both plate age and apparent thermal age are consistent with observed seafloor depth.
Praxis der Sozialen Arbeit in der medizinischen Rehabilitation
– Entwicklung einer empirisch fundierten Programmtheorie
Authors: Knoop Tobias , Scheiblich Nadja , Dettmers Stephan , Meyer-Feil Thorsten 
Published: November 2023

Zusammenfassung
Ziel der Studie Sozialarbeiter*innen sind Teil des
interprofessionellen Reha-Teams. Gleichwohl findet sich kaum Evidenz für
ihre Leistungen, ebenso fehlt eine theoretisch anschlussfähige
Beschreibung ihrer Praxis. An dieser Stelle setzt das Projekt
„Sozialarbeiterische Wirkmechanismen in der medizinischen Rehabilitation
(SWIMMER)“ an und zielt auf die Entwicklung einer Programmtheorie der
Sozialen Arbeit in der medizinischen Rehabilitation.
Methodik In dem qualitativen Forschungsprojekt wurden in zehn
Reha-Einrichtungen Interviews mit Sozialarbeiter*innen und
Leitungskräften sowie teilnehmende Beobachtungen durchgeführt
und Beratungsgespräche aufgezeichnet. Sampling und Auswertung
orientierten sich an der Grounded Theory.
Ergebnisse In den Einrichtungen konnten 42 Interviews, 14
Beratungsgespräche sowie die Protokolle von ca. 140 h
begleiteter Praxis ausgewertet werden. Die fallbezogene Praxis der Sozialen
Arbeit kann mit den drei Kernkategorien Arbeitsformen (z. B.
Informations- und Antragsarbeit), Interaktionssettings (Austausch mit
Rehabilitand*innen, dem Reha-Team und externen Akteuren) und Aufgaben
der Sozialdienste (z. B. Entwicklung beruflicher Perspektiven
beruflicher/sozialer Teilhabe, finanzielle und soziale Sicherung)
beschrieben werden. Als zentrales Charakteristikum der Praxis konnte die
Ko-Produktion mit den Rehabilitand*innen herausgearbeitet werden. Die
Konsequenzen sozialarbeiterischer Praxis wurden in Output- und Outcome-Faktoren
differenziert. Erstere sind z. B. gestellte Anträge oder
genannte Optionen der beruflichen Rückkehr. Zentrale Outcomedimensionen
sind die Teilhabe bzw. eine Perspektive auf diese und das Wohlbefinden der
Rehabilitand*innen.
Schlussfolgerungen Die als Ergebnis des Projekts empirisch fundiert
entwickelten Hypothesen der dem Modell zugrundeliegenden Wirkmechanismen sollten
in weiteren Studien näher untersucht werden. Zusätzlich zu einem
Nutzen für die Praktiker*innen eignet sich das Modell zudem zur
Einordnung der Ergebnisse wissenschaftlicher Studien.
Flow Cytometry in the Diagnostic Laboratory Workup of Acute Lymphoblastic Leukemias
Authors: Sharma Praveen , Srinivasan Tharageswari , Mallik Nabhajit 
Published: November 2023

AbstractAcute lymphoblastic leukemias (ALLs) are hematological neoplasms characterized by clonal proliferation of lymphoid blasts, which can be B- or T-cell type. Flow cytometric immunophenotyping is an integral component in establishing blast lineage during the diagnostic workup of ALLs, aiding in appropriate therapy, prognostication, and monitoring of the disease. The current review focuses on the utility of flow cytometry in the workup of ALLs, including the usefulness of various antibodies and pitfalls in diagnosis.
Flow Cytometric Ploidy Analysis in Acute Lymphoblastic Leukemia and Plasma Cell Myeloma
Authors: Bommannan Karthik 
Published: November 2023

AbstractIdentification of underlying cytogenetic (CG) aberrancies plays a significant role in risk stratification of hematological malignancies. These abnormalities can be due to aberrancies that affect the number or structure of chromosomes. Numerical chromosomal abnormalities are called aneuploidies, which result from either gain or loss of whole chromosomes. Ploidy assessment by CG is a laborious and less sensitive technique. With the aid of fluorescent nucleic acid binding dyes, the total DNA content and different phases of the cell cycle specific to any population of interest can be deciphered and analyzed by flow cytometry (FCM). DNA index (DI), a parameter derived by FCM DNA analysis, is equivalent to conventional CG-based ploidy assessment. In this study, the technical aspects and implications of FCM DNA assessment among patients diagnosed with acute lymphoblastic leukemia and plasma cell myeloma are discussed.
Exploring the effectiveness of fiscal decentralization in environmental expenditure based on the CO2 ecological footprint in urban China
Authors: Fang Guochang , Yang Kun , Chen Gang , Ren Xiaohang , Taghizadeh-Hesary Farhad 
Published: November 2023

AbstractUnder the fiscal decentralization of government environmental management, this paper investigates the relationship between local environmental protection expenditure (LEPE) and CO2 ecological footprint (CEF). Unlike conventional emissions-based greenhouse gas metrics, this research calculates per capita CEF for 253 Chinese cities, considering both carbon emissions and absorption. The dynamic spatial Durbin model demonstrates that LEPE not only reduces a city’s own CEF but also exerts a long-term influence on neighboring cities, signifying competitive dynamics among local governments in LEPE. This decentraliztion of environmental budget authority may yield adverse outcomes. Further analysis reveals an inverted U-shaped relationship between CEF and LEPE intensity, partly mirroring the environmental Kuznets curve. Different developmental stages should consider economic levels when allocating resources to environmental budgets. The low-carbon pilot policy strengthens LEPE, with varying effects across Chinese urban agglomerations, remaining consistent post-environmental protection tax introduction. These findings hold critical reference value for local policymakers aiming to collaboratively adjust market-oriented environmental policies.
Rich local knowledge despite high transience in an Arctic community experiencing rapid environmental change
Authors: Lennert Ann Eileen , van der Wal René , Zhang Jasmine , Hausner Vera Helene , Ancin Murguzur Francisco Javier , Miles Martin W. 
Published: November 2023

AbstractEnvironmental monitoring and long-term research produce detailed understanding, but its collective effort does not add up to ‘the environment’ and therefore may be difficult to relate to. Local knowledge, by contrast, is multifaceted and relational and therefore can help ground and complement scientific knowledge to reach a more complete and holistic understanding of the environment and changes therein. Today’s societies, however, are increasingly fleeting, with mobility potentially undermining the opportunity to generate rich community knowledge. Here we perform a case study of High Arctic Svalbard, a climate change and environmental science hotspot, using a range of community science methods, including a Maptionnaire survey, focus groups, interviews and cognitive mapping. We show that rich local knowledge on Svalbard could indeed be gathered through community science methods, despite a high level of transience of the local population. These insights complement environmental monitoring and enhance its local relevance. Complex understanding of Svalbard’s ecosystems by the transient local community arose because of strong place attachment, enabling environmental knowledge generation during work and play. We conclude that transience does not necessarily prevent the generation of valuable local knowledge that can enrich and provide connection to scientific understanding of the environment.
Sleep Deficits Inter-Link Lower Basal Forebrain–Posterior Cingulate Connectivity and Perceived Stress and Anxiety Bidirectionally in Young Men
Authors: Li Guangfei , Zhong Dandan , Li Bao , Chen Yu , Yang Lin , Li Chiang-Shan R 
Published: November 2023

Abstract

Background
The basal nucleus of Meynert (BNM), a primary source of cholinergic projections to the cortex, plays key roles in regulating the sleep-wake cycle and attention. Sleep deficit is associated with impairment in cognitive and emotional functions. However, whether or how cholinergic circuit, sleep, and cognitive/emotional dysfunction are inter-related remains unclear.

Methods
We curated the Human Connectome Project data and explored BNM resting state functional connectivities (rsFC) in relation to sleep deficit, based on the Pittsburgh Sleep Quality Index (PSQI), cognitive performance, and subjective reports of emotional states in 687 young adults (342 women). Imaging data were processed with published routines and evaluated at a corrected threshold. We assessed the correlation between BNM rsFC, PSQI, and clinical measurements with Pearson regressions and their inter-relationships with mediation analyses.

Results
In whole-brain regressions with age and alcohol use severity as covariates, men showed lower BNM rsFC with the posterior cingulate cortex (PCC) in correlation with PSQI score. No clusters were identified in women at the same threshold. Both BNM-PCC rsFC and PSQI score were significantly correlated with anxiety, perceived stress, and neuroticism scores in men. Moreover, mediation analyses showed that PSQI score mediated the relationship between BNM-PCC rsFC and these measures of negative emotions bidirectionally in men.

Conclusions
Sleep deficit is associated with negative emotions and lower BNM rsFC with the PCC. Negative emotional states and BNM-PCC rsFC are bidirectionally related through poor sleep quality. These findings are specific to men, suggesting potential sex differences in the neural circuits regulating sleep and emotional states.
A new function of thymol nanoemulsion for reversing colistin resistance in Salmonella enterica serovar Typhimurium infection
Authors: Sheng Qiushuang , Wang Nan , Zhou Yonglin , Deng Xuming , Hou Xiaoning , Wang Jianfeng , Qiu Jiazhang , Deng Yanhong 
Published: November 2023

Abstract

Background
Adjuvant addition of approved drugs or foodborne additives to colistin might be a cost-effective strategy to overcome the challenge of plasmid-mediated mobile colistin resistance gene emergence, which poses a threat in the clinic and in livestock caused by infections with Gram-negative bacteria, especially carbapenem-resistant Enterobacteriaceae.

Methods
Chequerboard assay was applied to screen the colistin adjuvants from natural compounds. The killing–time curve, combined disc test and membrane permeation assay were conducted to identify the synergy efficacy of thymol and colistin in vitro. Thin-layer chromatography (TLC), LC-MS and fluorescence spectra were used to indicate the interaction of thymol and MCR-1. The potential binding sites were then investigated by molecular simulation dynamics. Finally, a thymol nanoemulsion was prepared with high-pressure homogenization as the clinical dosage form.

Results
Thymol presented an excellent synergistic effect in vitro with colistin against Salmonella enterica serovar Typhimurium and Escherichia coli bacteria. Thymol addition, forming a complex with MCR-1, might interfere with the efficacy of MCR-1. Moreover, thymol strengthened colistin activity associated with potentiating membrane damage, destroying the biofilm and enhancing reactive oxygen species-mediated oxidative damage. Thymol nanoemulsion combined with colistin remarkably prevented the intestinal damage caused by S. Typhimurium infection, resulting in a survival rate higher than 60%.

Conclusions
This study achieved a promising thymol oral formulation as colistin adjuvant to combat S. Typhimurium infection, which could be used to extend the lifespan of colistin in clinical veterinary medicine.
SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice
Authors: Alhabsi Abdulrahman , Butt Haroon , Kirschner Gwendolyn K , Blilou Ikram , Mahfouz Magdy M 
Published: November 2023

Abstract
Plants employ sophisticated molecular machinery to fine-tune their responses to growth, developmental, and stress cues. Gene expression influences plant cellular responses through regulatory processes such as transcription and splicing. Pre-mRNA is alternatively spliced to increase the genome coding potential and further regulate expression. Serine/arginine-rich (SR) proteins, a family of pre-mRNA splicing factors, recognize splicing cis-elements and regulate both constitutive and alternative splicing. Several studies have reported SR protein genes in the rice genome, subdivided into six subfamilies based on their domain structures. Here, we identified a new splicing factor in rice with an RNA recognition motif (RRM) and SR-dipeptides, which is related to the SR proteins, subfamily SC. OsSCR106 regulates pre-mRNA splicing under abiotic stress conditions. It localizes to the nuclear speckles, a major site for pre-mRNA splicing in the cell. The loss-of-function scr106 mutant is hypersensitive to salt, abscisic acid, and low-temperature stress, and harbors a developmental abnormality indicated by the shorter length of the shoot and root. The hypersensitivity to stress phenotype was rescued by complementation using OsSCR106 fused behind its endogenous promoter. Global gene expression and genome-wide splicing analysis in wild-type and scr106 seedlings revealed that OsSCR106 regulates its targets, presumably through regulating the alternative 3'-splice site. Under salt stress conditions, we identified multiple splice isoforms regulated by OsSCR106. Collectively, our results suggest that OsSCR106 is an important splicing factor that plays a crucial role in accurate pre-mRNA splicing and regulates abiotic stress responses in plants.
Sacred Homelands
Authors: Smith Anthony D 
Published: November 2023

Abstract
Since its origins in the eighteenth-century ideal of a ‘return to nature’, the notion of a national homeland and of the nation as possessor of a unique and peculiar land has been central to nationalist ideologies. But here, too, the modern ideal has pre modern foundations. The contrast between ‘home’ and ‘abroad’, which we take for granted in our everyday speech and action, has a much longer history; and the idea of a special territory that is sanctified and set apart can be found in various parts of the world in different periods of history. Yet, only in the modern world has this older collective attachment to the homeland come to serve as another sacred foundation and cultural resource for the maintenance, and reinterpretation, of national identities. For modernists, the nationalist demarcation of territory as a homeland stems from the activities of the centralizing and reflexive state. These are mainly of two kinds: census taking and map making. Together, these modern activities enabled states to constitute the ‘body’ of the nation, to make the abstraction of the nation imaginable and palpable. Benedict Anderson points out that the census and map made it possible for nationalists to imagine and to maintain an
An Introduction to Children’s Literature
Authors: Hunt Peter 
Published: November 2023

Abstract
Passionately enjoyed as much by the young as by adults, children's literature is a vital part of Western culture. Attracting increasing scholarly attention, its indelible influences on most people's psyche attest to its widespread importance. Well-known for his work in the field, Peter Hunt here offers a history of children's literature--primarily British, although including American and other works as well--along with an assessment of its literary, sociological, and pedagogical roles. In addition, Hunt looks at the uses to which children's literature is put, the claims that are made for it, and the difference between children's and adults' ways of reading.
History and Histories
Authors: Hunt Peter 
Published: November 2023

Abstract
Children used books long before books were produced specifically for children—a fact that has given rise to the not very helpful argument that, as childhood was scarcely recognized or recognizable before the eighteenth century, all pre-1700 texts can be considered as (also) children’s texts. It would undoubtedly be interesting to discover what children made of Shakespeare or Reynard the Fox, but only if we could discover with any certainty what adults made of them. Because the twentieth-century reader sees a repressive, dry, crude, or simple text which would not appeal to the modern, liberated, multi-media child, we cannot assume that such texts would not appeal to the child of the time. None the less, historical evidence about childhood is lacking, and territorial lines have to be drawn somewhere, and so I propose to give only the briefest mention to texts produced before 1700 and, more radically, relatively little space to texts produced before about 1860.
The Long Weekend, 1920-1939
Authors: Hunt Peter 
Published: November 2023

Abstract
One answer is that children’s books reflect the world as we would like it to be; they react to the adult world. Just as, after the trauma of the Second World War, children’s books reacted by turning to fantasy, so, after the First, they looked towards various kinds of freedom. It may have been an effect of the change in attitudes to childhood; but, more concretely, there had been a sea-change, and the world after 1914 is much closer to ours than the world before. Certainly—although there are many exceptions—the tone of voice, the mode of telling, and the narrative contract between narrator and implied child reader of the children’s book that we recognize today were fully established.
A study of hospital fees in the Dominican Republic
Authors: Lewis Maureen A 
Published: November 2023

Abstract
Most public health systems in the developing world have been built in the belief that health care is a fundamental right; that the state must meet the health needs of the population; and that public services should be free at the point of use. Concerns over equal access and affordability have further enhanced the appeal of free-services policies. Yet despite the desirability of ensuring access to health services, recent economic events have made it clear − for the foreseeable future at least − that such policies are unaffordable for much of the developing world.
Economic determinants of demand for modern infant-delivery in low-income countries: the case of the Philippines
Authors: Brad Schwartz J , Akin John S , Popkin Barry M 
Published: November 2023

Abstract
A majority of births in the Philippines take place at home, and a significant proportion are either attended by a traditional midwife or friends and relatives of the mother. Women continue to choose this pattern of delivery despite large investments by the health sector in modern prenatal and obstetrical health services. Even though a large majority of the pregnant women have direct contact with modern delivery services, there still appears to be a preference for traditional home deliveries.
Quantity rationing and the demand of adults for medical care in rural C ô te D’Ivoire
Authors: Dor Avi , van der Gaag Jacques 
Published: November 2023

Abstract
The principal providers of health care in many developing countries are ministries of health and other public-sector institutions. Slow economic growth and record budget deficits in the 1980s have forced reductions in public spending. This has placed severe pressures on public health-care systems, unable to meet growing needs even during the economic growth periods of the 1960s and 1970s. Whereas funds for capital investment are often funded by international donors, recurrent costs are typically financed by the public treasury. Revenues from user charges usually contribute only about 10 per cent of recurrent expenditures (Ainsworth 1983; de Ferranti 1985). Consequently, little attention has been paid to market forces.