bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer-mediated Axonal Guidance of Sensory Neurons in a Microelectrode-based Innervation MPS
Authors: van der Moolen Matthijs , Lovera Andrea , Ersoy Fulya , Mommo Sacha , Loskill Peter , Cesare Paolo 
Published: October 2023

AbstractDespite recent advances in the field of microphysiological systems (MPS), availability of models capable of mimicking the interactions between the nervous system and innervated tissues is still limited. This represents a significant challenge in identifying the underlying processes of various pathological conditions, including neuropathic, cardiovascular and metabolic disorders. In this study, we introduce a compartmentalized 3D coculture system that enables physiologically relevant tissue innervation while recording neuronal excitability. By integrating custom microelectrode arrays into tailored glass chips microfabricated via selective laser-etching, we developed an entirely novel class of innervation MPSs (INV-MPS). This INV-MPS allows for manipulation, visualization, and electrophysiological analysis of individual axons innervating complex 3D tissues. Here, we focused on sensory innervation of 3D tumor tissue as a model case study since cancer-induced pain represents a major unmet medical need. The system was compared with existing nociception models and successfully replicated axonal chemoattraction mediated by nerve growth factor (NGF). Remarkably, in the absence of NGF, 3D cancer spheroids cocultured in the adjacent compartment induced sensory neurons to consistently cross the separating barrier and establish fine innervation. Moreover, we observed that crossing sensory fibers could be chemically excited by distal application of known pain-inducing agonists only when cocultured with cancer cells. To our knowledge, this is the first system showcasing morphological and electrophysiological analysis of 3D-innervated tumor tissuein vitro, paving the way for a plethora of studies into innervation-related diseases and improving our understanding of underlying pathophysiology.Significance StatementMPSs integrating innervated tissues are crucial as alternatives to animal models and to enhance the translation of non-clinical research to humans, as nerve fibers play a pivotal role in many organs. To address this need, we developed the INV-MPS, enabling parallelized electro-physiological recording from 3D cocultures of peripheral neurons with complex tissues. We demonstrate the ability of cancer spheroids to direct axonal growth and modulate excitability of nociceptive fibers in a 3D setting. We anticipate that this model will be applicable for closely monitoring innervation in various tissues, including somatic and visceral organs. The INV-MPS, hence, has tremendous potential for human-relevant drug discovery, creating opportunities for more ethical and clinically translatable approaches to study innervation in disease.
Unveiling the A-to-I mRNA editing machinery and its regulation and evolution in fungi
Authors: Feng Chanjing , Xin Kaiyun , Du Yanfei , Zou Jingwen , Xing Xiaoxing , Xiu Qi , Zhang Yijie , Zhang Rui , Huang Weiwei , Wang Qinhu , Jiang Cong , Wang Xiaojie , Kang Zhensheng , Xu Jin-Rong , Liu Huiquan 
Published: October 2023

AbstractA-to-I mRNA editing occurs during fungal sexual reproduction with an unknown mechanism. Here, we demonstrated that the eukaryotic tRNA-specific heterodimeric deaminase FgTad2-FgTad3, not typically associated with mRNA editing, is responsible for A-to-I mRNA editing inFusarium graminearum. This editing capacity relies on the interaction between FgTad3 and a sexual stage-specific protein called Ame1. The interaction emerged in Sordariomycetes. Key residues involved in the interaction have been identified. Expression and activity of FgTad2-FgTad3 are regulated through alternative promoters, alternative translation initiation, and post-translational modifications. FgTad2-FgTad3-Ame1 efficiently edits target mRNAs in yeasts, bacteria, and human cells, with significant implications for developing base editors in therapy and agriculture. This study reveals mechanisms, regulation, and evolution of RNA editing in fungi, emphasizing protein-protein interactions in controlling enzyme function.
Genomic Analysis of Diverse EnvironmentalAcinetobacterIsolates Identifies Plasmids, Antibiotic Resistance Genes, and Capsular Polysaccharides Shared with Clinical Strains
Authors: Tobin Liam , Jarocki Veronica M. , Kenyon Johanna , Drigo Barbara , Donner Erica , Djordjevic Steven P. , Hamidian Mehrad 
Published: October 2023

ABSTRACTAcinetobacter baumannii, an important pathogen known for its widespread antibiotic resistance, has been the focus of extensive research within its genus, primarily involving clinical isolates. Consequently, data on environmentalA. baumanniiand otherAcinetobacterspecies remain limited. Here, we utilised Illumina and Nanopore sequencing to analyse the genomes of tenAcinetobacterisolates representing six different species sourced from aquatic environments in South Australia. All ten isolates were phylogenetically distinct compared to clinical and other non-clinicalAcinetobacterstrains, often tens of thousands of SNPs from their nearest neighbours. Despite the genetic divergence, we identified pdifmodules (sections of mobilised DNA) carrying clinically important antimicrobial resistance genes in species other thanA. baumannii, including carbapenemaseoxa58,tetracycline resistance genetet(39) and macrolide resistance genesmsr(E)-mph(E).All of these pdifmodules were located on plasmids with high sequence homology to those circulating in globally distributedA. baumanniiST1 and ST2 clones. The environmentalA. baumanniiisolate characterised here (SAAb472; ST350) did not possess any native plasmids; however, it could capture two clinically important plasmids (pRAY and pACICU2) with high transfer frequencies. Furthermore,A. baumanniiSAAb472 possessed virulence genes and a capsular polysaccharide type analogous to clinical strains. Our findings highlight the potential for environmentalAcinetobacterspecies to acquire and disseminate clinically important antimicrobial resistance genes, underscoring the need for further research into the ecology and evolution of this important genus.IMPORTANCEAntimicrobial resistance (AMR) is a global threat to human, animal, and environmental health. Studying AMR in environmental bacteria is crucial to understand the emergence and dissemination of resistance genes and pathogens, and to identify potential reservoirs and transmission routes. This study provides novel insights into the genomic diversity and AMR potential of environmentalAcinetobacterspecies. By comparing the genomes of aquaticAcinetobacterisolates with clinical and non-clinical strains, we revealed that they are highly divergent yet carry pdifmodules that encode resistance to antibiotics commonly used in clinical settings. We also demonstrated that an environmentalA. baumanniiisolate can acquire clinically relevant plasmids and carries virulence factors similar to those of hospital-associated strains. These findings suggest that environmentalAcinetobacterspecies may serve as reservoirs and vectors of clinically important genes. Consequently, further research is warranted to comprehensively understand the ecology and evolution of this genus.
Biochemical and functional characterization of the p.A165T missense variant of mitochondrial amidoxime-reducing component 1 in HepG2 cells
Authors: Hou Wangfang , Watson Christian , Cecconie Ted , Bolaki Menaka N. , Brady Jennifer J. , Lu Quinn , Gatto Gregory J. , Day Tovah A. 
Published: October 2023

AbstractRecent genome-wide association studies have identified a missense variant p.A165T in mitochondrial amidoxime-reducing component 1 (mARC1) that is strongly associated with protection from all-cause cirrhosis and improved prognosis in nonalcoholic steatohepatitis (NASH). The precise mechanism of this protective effect is unknown. Substitution of alanine 165 with threonine is predicted to affect mARC1 protein stability and to have deleterious effects on its function. To investigate the mechanism, we have generated a knock-in mutant mARC1 A165T in human hepatoma HepG2 cells, enabling characterization of protein subcellular distribution, stability, and biochemical functions of the mARC1 mutant protein expressed from its endogenous locus. Compared to wild-type (WT) mARC1, we found that the A165T mutant exhibits significant mislocalization outside of its traditional location anchored in the mitochondrial outer membrane and reduces protein stability, resulting in lower basal levels. We evaluated the involvement of the ubiquitin proteasome system in mARC1 A165T degradation and observed increased ubiquitination and faster degradation of the A165T variant. In addition, we have shown that HepG2 cells carrying theMTARC1p.A165T variant exhibit lower N-reductive activity on exogenously-added amidoxime substratesin vitro. The data from these biochemical and functional assays suggest a mechanism by which theMTARC1p.A165T variant abrogates enzyme function which may contribute to its protective effect in liver disease.
Weighted burden analysis of rare coding variants in 470,000 exome-sequenced UK Biobank subject characterises effects on hyperlipidaemia risk
Authors: Curtis David 
Published: October 2023

AbstractA previous study of 200,000 exome-sequenced UK Biobank participants investigating the association between rare coding variants and hyperlipidaemia had implicated four genes,LDLR,PCSK9,APOC3andIFITM5, at exome-wide significance. In addition, a further 43 protein-coding genes were significant with an uncorrected p value of <0.001. Exome sequence data has become available for a further 270,000 participants and weighted burden analysis to test for association with hyperlipidaemia was carried out in this sample for the 47 genes highlighted by the previous study. There was no evidence to implicateIFITM5butLDLR,PCSK9,APOC3,ANGPLT3,ABCG5andNPC1L1were all statistically significant after correction for multiple testing. These six genes were also all exome-wide significant in the combined sample of 470,000 participants. Variants impairing function ofLDLRandABCG5were associated with increased risk whereas variants in the other genes were protective. Variant categories associated with large effect sizes are cumulatively very rare and the main benefit of this kind of study seems to be to throw light on the molecular mechanisms impacting hyperlipidaemia risk, hopefully supporting attempts to develop improved therapies.This research has been conducted using the UK Biobank Resource.
Impaired emotion recognition inCntnap2-deficient mice is associated with hyper-synchronous prefrontal cortex neuronal activity
Authors: Mohapatra Alok Nath , Jabarin Renad , Ray Natali , Netser Shai , Wagner Shlomo 
Published: October 2023

AbstractIndividuals diagnosed with autism spectrum disorder (ASD) show difficulty in recognizing emotions in others, a process termed emotion recognition. While human fMRI studies linked multiple brain areas to emotion recognition, the specific mechanisms underlying impaired emotion recognition in ASD are not clear, partially due to the lack of appropriate tests in animal models. Here, we employed an emotional state preference (ESP) task to show thatCntnap2-knockout (KO) mice, an established ASD model, do not distinguish between conspecifics according to their emotional state. We assessed brain-wide local-field potential (LFP) signals during various social behavior tasks and found thatCntnap2-KO mice exhibited higher LFP theta and gamma rhythmicity than did C57BL/6J mice, even at rest. Specifically,Cntnap2-KO mice showed increased theta coherence, especially between the prelimbic cortex (PrL) and the hypothalamic paraventricular nucleus, during social behavior. Moreover, we observed significantly increased Granger causality of theta rhythmicity between these two brain areas, across all types of social behavior. Finally, optogenetic stimulation of PrL pyramidal neurons in C57BL/6J mice impaired their social discrimination abilities, including ESP behavior. Together, these results suggest that increased activity of PrL pyramidal neurons and their augmented synchronization with specific brain regions are involved in the impaired emotion recognition exhibited byCntnap2-KO mice.
The impact of CEO power on corporate tax avoidance: the moderating role of institutional ownership
Authors: Oussii Ahmed Atef , Klibi Mohamed Faker 
Published: October 2023

Purpose
This study aims to investigate the relationship between chief executive officer (CEO) power and the level of tax avoidance of Tunisian listed companies. It also examines the moderating role of institutional ownership in this association.

Design/methodology/approach
The sample comprises 306 firm-year observations of companies listed on the Tunis Stock Exchange during the 2013–2020 period.

Findings
The results indicate that CEO power reduces tax avoidance levels. Moreover, the relationship between CEO power and tax avoidance is more pronounced in the presence of institutional ownership, suggesting that CEOs act less opportunistically when monitored by institutional investors, which results in a reduction in tax avoidance.

Practical implications
This study suggests that CEO power and institutional shareholders’ influence are important factors in determining firms’ avoidance behavior. This study has significant implications for shareholders and regulatory bodies. Indeed, shareholders apprehend the impact of appointing a powerful CEO on tax avoidance practices. This study may also provide regulators with new insights into the influence of CEO power dimensions and institutional ownership on tax aggressiveness.

Originality/value
This study fills the gap in the accounting literature by investigating how CEO power may impact tax avoidance behavior and provides empirical evidence on the moderating impact of institutional ownership on this relationship in an emerging economy context characterized by a weakly protected investor setting.
Nature-love and sustainable consumption behaviors: does the construal level of psychological distance matter?
Authors: Elsotouhy Mohamed M. , Ghonim Mohamed A. , Khalifa Nada , Khashan Mohamed A. 
Published: October 2023

PurposeDespite the importance of emotional variables in shaping individuals' consumption behavior, nature-love still needs to be addressed concerning various aspects of sustainable consumption behavior (SCB). Considering the dimensions of nature-love, this study aims to investigate the effect of passion-for-nature, intimacy-with-nature and commitment-to-nature on SCB. Furthermore, this study aims to incorporate the construal levels of psychological distance (PD) as a moderating variable between the tested variables to add a more in-depth understanding.Design/methodology/approachData was collected from a sample of 311 individuals from Egypt using the snowball sampling method and the ten-time rule technique. The data was analyzed using partial least squares-structural equation modeling (PLS-SEM).FindingsThe findings indicate that passion-for-nature and intimacy-with-nature have a significant positive effect on green purchasing, reusability and recycling. On the other hand, commitment-to-nature has a significant positive effect on both green purchasing and reusability. Additionally, a high PD acts as a moderator between the relationships tested. The findings have been discussed in terms of their theoretical and practical implications.Originality/valueTo the best of the authors’ knowledge, this is the first study to integrate PD as a moderator between the relationships tested. Additionally, this paper is the first empirical research investigating these relationships in developing economies.
The economics of emissions in rice production: a survey-data-driven approach in Vietnam
Authors: Chu Long 
Published: October 2023

PurposeThis paper aims to focus on scrutinizing the economics of greenhouse gas (GHG) emissions in Vietnam's rice production sector.Design/methodology/approachUsing surveyed data from household rice producers, the smallest available production scale, the author delves into the economics of GHG emissions, constructs a data-driven bottom-up marginal abatement cost curve for Vietnam’s rice production, and evaluates the impacts of carbon pricing on production outputs and GHG emissions.FindingsThe author’s estimates reveal that the average profit earned per tonne of GHG emissions is $240/tCO2. Notably, the profit earning per tonne of GHG emissions varies substantially across producers, indicating significant opportunities for improvement among low-efficiency producers. The analysis suggests that a reasonable carbon price would yield a modest impact on the national rice output. The quantitative analysis also reaffirms that the primary driver of GHG emissions in Vietnam’s rice production stems from non-energy inputs and industrial processes rather than the utilisation of energy inputs, emphasizing the importance of improving cultivation techniques.Originality/valueThis research is original.
Identifying the technological position of semiconductor laser developers: a patent-based analytical perspective
Authors: Chang Shu-Hao 
Published: October 2023

Purpose
Defining and validating a map of related technologies is critical for managers, investors and inventors. Because of the increase in the applications of and demand for semiconductor lasers, analyzing the technological position of developers has become increasingly critical. Therefore, the purpose of this study is to adopt the technological position analysis to identify mainstream technologies and developments relevant to semiconductor lasers.

Design/methodology/approach
Correspondence analysis and k-means cluster analysis, which are data mining techniques, are used to reveal strategic groups of major competitors in the semiconductor laser market according to their Patent Cooperation Treaty (PCT) patent applications.

Findings
The results of this study reveal that PCT patent applications are generally obtained for masers, optical elements, semiconductor devices and methods for measuring and that technology developers have varying technological positions.

Originality/value
Through position analysis, this study identifies the technological focuses of different manufacturers to obtain information that can guide the allocation of research and development resources.
Socioeconomic status of international students and its relation to the brain drain: evidence from Greek PhD holders
Authors: Labrianidis Lois , Sykas Theodosis , Sachini Evi , Karampekios Nikolaos 
Published: October 2023

PurposeThe study examines potential differences in socioeconomic status (SES) and emigration patterns between Greek international students (IS) and non-international students (non-IS) and their relationship to the brain drain from Greece.Design/methodology/approachThe study draws on a unique database including all the Greek PhD holders and provides detailed information on their SES and mobility patterns. Furthermore, an individual-level SES index is constructed including both human capital and socioeconomic indicators to estimate the magnitude of the brain drain in terms of the SES that emigrated abroad between 1,985 and 2,018.FindingsFirst, Greek IS have a higher educational, professional and economic status compared to Greek non-IS. Moreover, they exhibit a more international profile, inasmuch as they are more likely to remain abroad after graduation to seek employment. Second, the magnitude of the brain drain in terms of SES emigrated abroad (22.5% of the total) is greater than in terms of individuals who moved abroad (13.4% of the total). Specifically, the SES that outflows with an additional Greek skilled emigrant (that is, an additional IS and non-IS residing abroad) is 1.1 times greater than the SES that remains in Greece with an additional non-IS residing in Greece.Originality/valueThe study contributes to the scientific discussion that relates the SES of IS and highly skilled migrants to brain drain and fills the gap in the relevant literature.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-09-2022-0607.
“Not so good vibrations”: five collaborative autoethnographic accounts of Brian Wilson, his life, music, rock “n” recovery’
Authors: Hopkinson Patrick , Niklasson Mats , Bryngelsson Peter , Voyce Andrew , Carson Jerome 
Published: October 2023

Purpose
The purpose of this paper is to provide an analysis of the life of the musician Brian Wilson from five different perspectives.

Design/methodology/approach
The authors used a mixed method of collaborative autoethnography, psychobiography and digital team ethnography to try and better understand the life and contributions of Brian Wilson.

Findings
Each of the five contributors provides different insights into the life and music of Brian Wilson.

Research limitations/implications
While the focus of this paper is on a single individual, a case study, the long and distinguished life of Brian Wilson provides much material for discussion and theorising.

Practical implications
Each individual presenting to mental health services has a complex biography. The five different contributions articulated in this paper could perhaps be taken as similar to the range of professional opinions seen in mental health teams, with each focusing on unique but overlapping aspects of the person’s story.

Social implications
This account shows the importance of taking a biological-psychological-social-spiritual and cultural perspective on mental illness.

Originality/value
This multi-layered analysis brings a range of perspectives to bear on the life and achievements of Brian Wilson, from developmental, musical, psychological and lived experience standpoints.