bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lysine production from hydrocarbon by micrococcus species
Authors: Chatterjee M. , Chatterjee S. P. 
Published: December 2004

AbstractA bacterium isolated from Assam (India) soil was found to accumulate L‐lysine in the mineral salt‐hydrocarbon medium and identified to be a strain of Micrococcus luteus. The strain is able to grow and accumulate l‐lysine in a purely synthetic medium, but supplementation of the synthetic medium with casamino acid or yeast extract or both, improves the yield. The entire fermentation period can be divided into a growth phase and a production phase, which can be prolonged by adjustment of the pH to the neutral range. Among the different hydrocarbon and nitrogen sources tested straight run gas oil (47percnt;) and ammonium sulphate (0.4%), respectively, were found most suitable.Erythromycin at 1 μg/ml level inhibited growth bu¸t stimulated lysine excretion. An inoculum level of 10% (v/v) of the medium was optimal for lysine production. The yield of lysine under optimal conditions was found to be 3.25 g per litre of the medium. Lysine has been isolated in crystalline form from the fermented broth by ion exchange resin chromatography and found to be pure sample of L‐isomer.
Die chemische Hydrolyse von Reisschalen und das Wachstum einer Candida tropicalis‐Kultur auf den Reaktionsprodukten
Authors: Gonzales‐Valdes I. , Knackmuss K.‐P. 
Published: December 2004

AbstractThe kinetics of the prehydrolysis of rice hulls where investigated with different concentrations of H2SO4 and temperatures by modified ARRHENIUS–equation in relation to the degradation of pentosans and the formation of reducing sugars. The determination of the activation energy, the frequency‐factor and the orden of reaction in relation to the concentration of the acid results for the formation of the reducing sugars in 60.3 kJ/mol, 5.28 ×. 107 and 1.76, for the degradation of the pentosans in 59‐ and 69.9 kJ/mol, 3.08 × 107 and 6.03 times; 107 as well as 0.76 and 0.85The discontinuous growth of the yeast Candida tropicalis QML 7601, isolated from a biotop in Cuba, on the products of the prehydrolysis of the rice hulls with H2SO4 (0.1‐ and 0.2 N) and different mixtures of H2SO4 and HNO3 yield values of μmax = 0.3 – 0.6 h‐1 in relation to the conditiones.
Zur Bedeutung der wahren maximalen spezifischen Wachstumsrate
Authors: Koné S. , Behrens U. 
Published: December 2004

AbstractIn this report the biokinetic parameter μmax, m and Ymax for the denitrification of a medium containing acetat as C‐source and a soft coal gasification water containing volatile fatty acids as main components have been compared. An expression for a true maximum specific growth rate is proposed (μmaxw = μmax + mYmax) and it could be shown that despite different values for μmax the values for μmaxw correspond fairly well.
Activity of diaminopimelic acid decarboxylase in bacteria producing lysine
Authors: Sen S. K. , Chatterjee M. , Chatterjee S. P. , Banerjee A. K. 
Published: December 2004

AbstractActivity of DAP‐decarboxylase in lysine overproducing strains of Micrococcus luteus, M. varians and Arthrobacter globiformisLb reached the highest level during the end of the exponetial phase of growth and remained at the same high level during the stationary phase of growth when major bulk of lysine was accumulated. In comparison in a lysine non‐producing strain of Arthrobacter globiformis I 4 the activity of the same enzyme was low. DAP‐decarboxylase of these three lysine overproducers has two specialities, persistence during the stationary phase and insensivity to repression by exogenous lysine.
Metabolic Features of Methane‐ and Methanol‐Utilizing Bacteria
Authors: Trotsenko Y. A. 
Published: December 2004

AbstractInvestigations of a wide range of methane‐ and methanol‐utilizers showed a striking versatility of their metabolism dependent on the genotype and growth conditions. A correlation between pathways of carbon and nitrogen metabolism was found. It was most stringent in obligate methane‐utilizers: the hexulosephosphate pathway bacteria assimilated NH3 by the reductive amination of α‐ketoglutarate or pyruvate whereas the serine pathway bacteria used the glutamate cycle (glutamine synthetase + glutamate‐oxoglutarate aminotransferase).Multiple enzymic lesions were found in central metabolism of obligate methylotrophs, i.e. the absence of the enzymes of glycolytic and pentosephosphate pathways, gluconeogenesis, citric acid cycle and glyoxylate shunt. These metabolic blocks were not so profound and could be compensated in restricted and facultative methylotrophs during heterotrophic growth.The average levels of exogenous CO2 fixation in methylotrophic bacteria with the hexulosephosphate, serine and ribulosebisphosphate pathways were found to be 10, 30 and 80% of their total cell carbon, respectively.These results served as a basis for biotechnological applications of metabolic potential of methylotrophs (production of biomass, polysaccharides and enzymes as well as for microbiological treatment of industrial waters containing toxic C1‐ and Cn‐compounds).
Zur Maßstabsübertragung von aeroben mikrobiologischen Prozessen Teil I. Stofftransport
Authors: Kudrewizki F. 
Published: December 2004

AbstractBecause the interior scale of turbulence in reinforced bioreactors is essentially greater than the particles of bacteria or yeasts, the mass transfer occurs at the particles only in consequence of the molecular diffusion and depends on the concentration of the soluted material only. As a decided criterion for the transmission of scale in such processes is considered the volumetric oxygen transfer coefficient KLaThis article practises a theoretical analysis of the characteristic hydrodynamic conditions in bubble columns and in agitators: the content of gas, the specific area of mass transfer and the coefficient of mass transfer KL. These conditions are joined with the evaluation of the KLa value. Resultant the data of calculation hence it follows a computational determination of the KLa value by means of the physical matter values, the construction of devises and the process conditions.
Die Verwendung thermodynamischer und kinetischer Gesetzmaäßigkeiten zur Interpretation des Begriffes, physiologischer Zustand bei Hefepopulationen, Teil I: Grundlagen und Modelle
Authors: Glombitza F. , Heinritz B. , Ringpfeil M. 
Published: December 2004

AbstractThe paper wants to give a definition of the “physiological” state and a description of the microorganism growth by stochastic, parametric and deterministic equationsThe growth process is divided in different phase and described by the equations of ZOTIN/ZOTINA and BERTALNFFYA linear equation systems of irreversible thermodynamics is used for characterizing growth processes. The time and mass differences are choosen as driving forcesThis description of growth is valid only in the case of synchronously growing cellsOn the other hand, the physiological state of asynchronously growing cultures can be characterized by statistic average values.
Fraktionierung von Inhaltsstoffen der Zuckerrübenmelasse, die toxisch für die Citronensäureproduktion mit Aspergillus niger sind
Authors: Żakowska Z. , Jałocha A. 
Published: December 2004

AbstractThe toxic fractions were isolated from unsuitable molasses,not accepted for citrie acid fermentation. When the strains of A. niger: Z‐208, R‐8, P‐16, R‐65/4, all good producers of citric acid, were cultivated on the medium containing such molasses, the growth patterns were altered and the strains lost their acid‐forming properties.After dialysis of the molasses samples fractions of low and high molecular weight were obtained but the latter had no effect neither on growth nor on fermentation patterns. The low molecular part, responsible for the toxicity of molasses against A. niger, was fractionated with Sephadex G‐25.In such a way a low coloured fraction which inhibited the growth of A. niger strains could be separated. However there were no toxic compounds in the fractions with the highest values of absorbancy. The susceptibility of the strains,to some extent, depended on their ability for adaptation to the toxic fractions of molasses.
Ein neuer CO2‐Inkubator für die biologische Forschung
Authors: Beckmann D. , Metze J. , Rheinläunder H. 
Published: December 2004

AbstractThe engineers of the Centre for Scientific Instrument Manufacture of the Academy of Science of the GDR have engineered a bench incubator for the requirements of tissue culture and other biological research. The chamber made of corrosion resistant metal has a volume of about 100 litres. The cultures standing at three shelves are areated horizontal by two radial ventilators. The incubator equipped with automatic CO2‐, temperature‐ and humidity measuring‐ and regulation systems is distinguished by a new method of sterilization.
Dynamic processing in biotechnical systems
Authors: Heinritz B. , Bley T. , Rogge G. , Stichel E. , Ringpfeil M. 
Published: December 2004

AbstractThe adaptive feeding of nutrients especially of carbon and energy sources according to the demand of cells in different cell states during continuous or semicontinuous cultivation is called dynamic processing.The deduction of dynamic process control concepts is aimed at improving the efficiency of biological substrate conversions into cell mass and other reaction products.A growing number of results allows us to postulate that the principle of dynamic processing should be generally applied in biotechnical processes independent of the type of cells and substrates as well as the nature of products.The basis of the deduction of dynamic process control concepts is the exact knowledge of the dependences of efficiency and rate of product synthesis on cell states during the development of cell populations.
Biosorption of mercury by microorganisms
Authors: Glombitza F. , Iske U. , Gwenner Chr. , Krishnan M. R. V. 
Published: December 2004

AbstractBiomasses are able to sorb great amounts of mercury ions from water solutions.Sorbed concentrations are dependent on the pH‐value, the strain of microorganisms and the substrate used for cultivation.The uptake of mercury takes place at a high reaction rate and is completed after few minutes. A comparison between maximum amounts of uptake concentration and theoretical maximum amounts with a total sataration of the surface leads to the assumption that mercury is bound to definite sites.
Stickstoff als Leitelement für die Untersuchung von Fermentationsprozessen. Anwendungsmöglichkeiten eines Ammoniummeßgerätes
Authors: Otto G. , Pitzler J. , Pitzler K. , Weichert D. 
Published: December 2004

AbstractThis paper presents the application of a new developed measuring device for continuous determination of ammonium concentration especially in the field of biotechnology. Kinetic measurements of changes of ammonium concentration during stationary and instationary growth of microorganisms allows to quantify such interesting parameters as nitrogen consumption rate rN, productivity rx, specific growth rate ß, adaption time, diauxic behavior and mass content MF of fermenter etc.The results received by the combination of appropriated methods with the ammonium measuring device are not limited to biotechnology, so other applications in the field of chemistry, agriculture, technology, waste water industry etc. are possible.
Influence of yeast‐cell state on efficiency of ethanol production
Authors: Heinritz B. , Ghildyal N. P. , Rogge G. , Hanschmann G. , Ringpfeil M. 
Published: December 2004

AbstractEthanol synthesis was initiated in aerobically pre‐cultivated synchronized yeast populations by interrupting aeration and adding carbon substrate. Synchronization of yeast‐cell functions was carried out by aerobic phased cultivation.Ethanol synthesis was more effective if it was closely connected with aerobic cell‐mass synthesis and cell proliferation. Thus, anaerobic continuous ethanol production with cell recycling could be intensified by replacement of aged cells in single‐cell state by aerobically pre‐cultivated budding cells.
Inhibierungserscheinungen bei der alkoholishchen Gärung III. Der Einfluß des osmotischen Druckes auf die Lebensfähigkeit von Saccharomyces cerevisiae
Authors: Richter K. , Becker U. 
Published: December 2004

AbstractThe influence of the osmotic pressure on the viability of yeast cells was studied in batch processes. It could be found, that the viability is a function of the total osmotic pressure obtained by adding the partial osmotic values of the principal ingredients saccharose, ethanol and salts dissolved in the medium. At optimum process conditions (pH, T, etc.) the cells can tolerate the osmotic pressure up to fixed value π1. Above π1 the viability decreases linearly and upward of a second threshold value π2 nonlinearly. In the case of the used strain Saccharomyces cerevisiae Hansen Sc 5 the values of the both points are π1 = 25 atm and π2 = 47 atm.
Untersuchungen zur Überproduktion von Tryptophan und anderer Indolderivate durch Hansenula henricii
Authors: Bode R. , Birnbaum D. 
Published: December 2004

AbstractThe cell pools of tryptophan and anthranilate, the excretion of indole‐containing metabolites, and the levels of the enzymes of aromatic amino acid biosynthesis have been determined in regulatory mutants of Hansenula henricii. The strain Hg 48‐2‐M8 produces indoles with a maximum specific productivity of 0.37 mg/g · h at a maximum specific production value of 21 mg/g dry cell weight. This methyl‐tryptophan resistant mutant possess an anthranilate synthase, whose inhibition by tryptophan is reduced. The best conditions for production of indoles are the following: 1% glucose as C‐source; ammonium as N‐source; pH value smaller than 4. We found that under various growth conditions 25–60% synthesized indole‐containing metabolites consists of tryptophan.
Influence of antibiotics and trace salts on lysine production by arthrobacter globiformis
Authors: Sen S. K. , Chatterjee S. P. 
Published: December 2004

AbstractA hydrocarbon utilizing strain of Arthrobacter globiformis Lb isolated from local soil has been found to yield lysine 3.4 g l−1, keeping the medium optimal for pH, C‐ and N‐sources. Addition of antibiotics and micronutrients to that optimal media stimulated cell growth and enhanced lysine yield.
Antiphytovirale Aktivität von lipophilen Fraktionen aus der Hefe Lodderomyces elongisporus IMET H 128
Authors: Voigt B. , Müller H. , Schuster G. 
Published: December 2004

AbstractLipid extract isolated from biomasses of Lodderomyces elongisporus IMET H 128 grown on gas oil 7(B.p. 240–380°C) and fractions of the lipid extract (acetone soluble and fatty acid fractions) reduced the concentration of potato virus X in inoculated as well as in secondarily infected leaves markedly. The antiphytoviral inactive phosphatide fraction was converted into a fraction with high activity by partial hydrolysis with SO2 as acting agent.
Isolierung von Bordetella‐Phagen aus Bakterienstämen der Impfstoffproduktion
Authors: Mebel S. , Rustenbach S. , Lapaeva I. A. 
Published: December 2004

AbstractIt has been demonstrated that strains of Bordetella pertussis used for vaccine production contain temperate phages. It can be conducted from many experiments performed in our laboratory. that 10–100 phages per 1010 bacteria are released. However, the production of bacterial mass is not markedly influenced by lysogeny.Strains of Bordetella bronchiseptica used for production of vaccine against Rhinitis atrophicans of pigs have temperate phages too. These phages may cause a complete lysis during a submerse cultivation.The phages of Bordetella pertussis and Bordetella bronchiseptica can be propagted on Bordetella parapertussis.
Proteolysis upon dehydration of yeasts saccharomyces cerevisiae
Authors: Damberga B. E. , Vilcáuns A. P. , Laivenieks M. G. , Beker M. J. 
Published: December 2004

AbstractThe effect of dehydration on proteolysis and activity of proteases A, B and C in the cells of baker's yeast Saccharomyces cerevisiae was investigated. It can be concluded, that under investigated conditions of yeast Saccharomyces cerevisiae drying a decrease of proteases activity takes place. In cells a limited proteolysis takes place which is indicated by an increase in amino nitrogen content and a decrease of tryptophane synthase activity. Adding the protease inhibitor to yeast suspension prevents decrease of tryptophane synthase activity upon dehydration.
Study and theoretical modelling of the drying of the aminoacids – The products of microbial synthesis
Authors: Saduikov R. A. , Migunov V. V. , Karpov A. M. , Golubev L. G. , Pobedimsky D. G. 
Published: December 2004

AbstractSome properties of the pure aminoacids – the products of the microbiological synthesis as an object of drying are established by the experiments. It is shown that the vacuum‐oscillating drying method is the effective solution of the technological problem of drying the Leucine, Isoleucine, Triptophan and Threonin. The mathematical model of this process is elaborated, whose adequacy is verified by experiments. This model may be used for technological calculations and for technology optimization.
Vergleichende Untersuchungen von verschiedenen Trägermaterialien bezüglich ihrer Eignung für die chemische Oligonucleotidsynthese nach dem Phosphotriesterverfahren
Authors: Weiss R. , Birch ‐Hirschfeld E. , Witkowski W. 
Published: December 2004

AbstractA number of various oligonucleotides was synthesized on different supports, as well in 3′‐5′, as in 5′–3′ direction. Self made styrene grafted teflones showed some advantages in comparison with commercially available supports.
Analyse von zwei Legumin‐Genfamilien der Ackerbohne (Vicia faba L.)
Authors: Wobus U. , Bäumlein H. , Bassüner R. , Jung R. , Müntz K. , Saalbach G. , Weschke W. 
Published: December 2004

AbstractDNA clones representing two subfamilies A and B of legum in genes and a recombinant phage containing a complete legumin B gene have been isolated and characterized by DNA sequencing. A DNA fragment harbouring the legumin B gene and adjacent sequences was used for Ti‐mediated transfer into tobacco cells.
Some theoretical considerations on overflow production of metabolites
Authors: Babel W. 
Published: December 2004

AbstractThe overflow production of metabolites appears to be an energy spilling process in terms of life because part of the energy of the primary substrate remains in the metabolite produced. The other part of energy, which is liberated as reducing equivalents and/or ATP along the way to the product, must be wasted. This part is discussed to be responsible for the discrepancies between the theoretically possible and experimentally obtained product yields, because for the wasting process substrate or product are consumed. By reducing the amount of this superfluous energy the product yield should be increased. The auxiliary substrate concept occurs to be an appropriate method.
Partikelelektrophoretische Charakterisierung der Oberflächeneigenschaften von alkanutilisierenden Hefezellen: Chemische Zusammensetzung und Tensidadsorption
Authors: Lerche K.‐H. , Kretzschmar G. 
Published: December 2004

AbstractBy using particle electrophoresis and quantitative analysis the interaction of anionic surfactants (sodium dodecylculfate, sodium decylbenzene sulfonate) with a hydrocarbon‐grown yeast and a commercial baker's yeast has been investigated to obtain further informations about the chemical composition of the surface region (3–6 nm) of the cell wall of this yeasts. A correlation is found between the chemical composition and the different adsorption behaviour of various batches of the same yeasts. It was found that surface‐localized hydrophobic glycoproteins, probably proteophosphomannan are responsible for the strong pH dependent adsorption behaviour of a typical Candida species.In contrast the cell surface of a typical Saccharomyces species was strongly hydrophilic and showed no surfactant adsorption.This can be explained by the presence of a polysaccharide probably phosphomannan in the surface region only.The implication of proteins in the adsorption process is confirmed by model experiments too.The models for the yeast cell wall proposed by LAMPEN and KIDBY et al. were critically discussed in terms of our results.
Der neue hemmhofmeßautomat HZAA‐3 des autoselect‐systems
Authors: P. Mühlig , Bauer E. , Schicht G. , H. Günther , Harz D. 
Published: December 2004

AbstractFor the automatical measuring of inhibition zones on bioassay plates a new zone reader of the Autoselect‐System has been developed. This third type is described. It combines all advantages of the preceding types as, for instance, the special principle of measuring and the transport of the plates. However, details of the function, the service, and the design have been improved.
Study on the enzyme hydrolysis of waste photoemulsions
Authors: Krysteva M. A. , Peev G. A. , Yotova L. K. 
Published: December 2004

AbstractConditions for silver recovery from waste photoemulsions by means of the enzymes trypsin and alkaline protease hydrolysis of the gelatine and the mechanism of process were investigated. The degree of silver regeneration is depending on time during the first minutes. Temperature has a decisive significance only during the initial minutes of the process.The sedimentation of silver chloride from the enzyme‐degraded photoemulsions depends strongly on the valency of the anions present in the solution.
Naturwissenschaftliche und technologische Aspekte eines mechanisch‐chemischen Strohaufschlußverfahrens
Authors: Mietz M. , Berg F. , Jacopian V. , Philipp B. , Sauer I. 
Published: December 2004

AbstractTo enhance the digestibility of wheat straw as an animal feedstock a partial delignification by action of 1percnt; aqueous alkali in combination with mechanical pre‐ and after‐treatment steps is proposed. On the basis of in vitro digestibility by cellulase the influence of various process parameters on the effect obtained is summarized. Sheep feeding studies comparing original and chemomechanically pretreated straw confirm the suitability of the process‐principle proposed for upgrading cereal straw. The practical feasibility of the process is considered with regard to animal nutrition and technology.
Prototrophic hybrids of bakers yeast. I. Selection of haploids and diploids of prototrophic yeast
Authors: Szopa J. S. , Ołtuszak E. 
Published: December 2004

Abstract153 haploids, including 8 prototrophs, 12 biotin prototrophs and 4 biotin auxotrophs were isolated from Y, F and FB strains of the baker's yeast Saccharomyces cerevisiae. Remaining haploids were dependent on vitamins of B group other than biotin.Obtained haploids were characterized by large cell sizes, a or α mating type, the ability to fermentation of sugars and the assimilation of non‐fermented carbon sources.Haploids obtained from the yeast of Y and FB strains a good growth in the synthetic medium.Prototrophic haploids, prototrophic haploids with biotin auxotrophs and biotin prototrophs with biotin auxotrophs were crossed. 89 prototrophic hybrids capable of growth in synthetic medium without vitamins were selected out of 178 hybrids obtained.Hybrids are characterized by features typical for baker's yeast; however, not all of them are capable of sporulation.As result of selection, prototrophic hybrids and 16 hybrids characterized by a good increase of biomass in molasses medium were chosen.The efficiency of the biomass of hybrids designated as YY 3040/2, YY 3040/3 and FFB 1910/2 is considerably higher than one obtained from the cultivation of the industrial strain French Mautner (Mf).All hybrids possess adequate enzymatic activity in anaerobic metabolism of saccharose and maltose.Selected prototrophic hybrids were sent out to two yeast factories in the country for experimental propagation.