bluuebunny/crossref_metadata_2025_split huggingface dataset:
Orchestrating resources with suppliers for product innovation
Authors: Chirico Francesco , Naldi Lucia , Hitt Michael A. , Sieger Philipp , Sirmon David G. , Xu Kai 
Published: October 2023

AbstractHow orchestrating external, supplier‐provided resources affects product innovation is an important question. While product innovation is essential to achieve a competitive advantage, it is costly as it requires significant investments. It thus puts a severe strain on firm resources, which is particularly critical for resource‐scarce small–medium enterprises (SMEs). Therefore, these firms must combine their own resources with those of external parties, particularly suppliers, to enhance their product innovation. However, knowledge about how orchestrating these resources affects product innovation is limited, because empirical studies on resource orchestration have largely focused on firm‐internal resources; furthermore, there is ambiguity regarding the extent to which drawing on external resources is beneficial. In addition, the conditions that affect the resource orchestration–product innovation relationship remain unexplored. Therefore, we focus on supplier logistics integration (SLI), an important resource orchestration action referring to the orchestration of external, supplier‐provided resources; we draw on the resource orchestration framework and the related work on organizational rigidity to theorize that there is an inverted U‐shaped relationship between SLI and product innovation. Additionally, we suggest that learning orientation and environmental dynamism mitigate rigidities associated with high levels of SLI and thus facilitate the translation of SLI into product innovation. Testing our hypotheses with data from Swedish SMEs supports our theorizing and provides important contributions. Most importantly, we extend the resource orchestration framework to include the focus on external, supplier‐provided resources, thereby advancing our knowledge and understanding of resource orchestration for product innovation in SMEs.
Short‐term effects of canagliflozin on glucose and insulin responses in insulin dysregulated horses: A randomized, placebo‐controlled, double‐blind, study
Authors: Lindåse Sanna , Nostell Katarina , Forslund Anders , Bergsten Peter , Bröjer Johan 
Published: October 2023

AbstractBackgroundDecreasing hyperinsulinemia is crucial in preventing laminitis in insulin dysregulated (ID) horses. Complementary pharmacological treatments that efficiently decrease postprandial hyperinsulinemia in ID horses are needed.ObjectivesCompare short‐term effects of canagliflozin vs placebo on glucose and insulin responses to an oral sugar test (OST) as well as the effects on body weight and triglyceride concentrations in horses with ID.AnimalsSixteen privately‐owned ID horses.MethodsA single‐center, randomized, double‐blind, placebo‐controlled, parallel design study. The horses were randomized (ratio 1:1) to either once daily PO treatment with 0.6 mg/kg canagliflozin or placebo. The study consisted of an initial 3‐day period for obtaining baseline data, a 3‐week double‐blind treatment period at home, and a 3‐day follow‐up period similar to the initial baseline period but with continued double‐blind treatment. Horses were subjected to an 8‐sample OST in the morning of the third day on both visits.ResultsMaximal geometric least square (LS) mean insulin concentration (95% confidence interval [CI]) during the OST decreased after 3 weeks of canagliflozin treatment compared with placebo (83.2; 55.4‐125.0 vs 215.2; 143.2‐323.2 μIU/mL). The geometric LS mean insulin response (insulin AUC0‐180) for canagliflozin‐treated horses was >66% lower compared with placebo. Least square mean body weight decreased by 11.1 (4‐18.1) kg and LS mean triglyceride concentrations increased by 0.99 (0.47‐1.5) mmol/L with canagliflozin treatment.Conclusions and Clinical ImportanceCanagliflozin is a promising drug for treatment of ID horses that requires future studies.
Long‐term outcome of epileptic dogs treated with implantable vagus nerve stimulators
Authors: Harcourt‐Brown Thomas R. , Carter Michael 
Published: October 2023

AbstractBackgroundThe long‐term effect of implantable vagus nerve stimulators (VNS) on seizures has not been evaluated in epileptic dogs.ObjectivesReport seizure frequency in medication‐resistant epileptic dogs before and after VNS implantation.AnimalsTwelve client‐owned dogs with idiopathic epilepsy and >1 seizure day per 3 weeks despite 3 months of appropriate use of 2 antiseizure medications and seizure diaries maintained 6 months before and >12 months after VNS implantation.MethodsUncontrolled, open‐label, before and after study. Mean monthly seizures and inter‐seizure periods obtained from contemporaneous seizure diaries in the 6 months before implantation were compared with 0 to 6 months, 7 to 12 months, and subsequent 12‐month periods after implantation. The number of dogs with >50% decrease in seizure frequency, >3 times increase in inter‐ictal period interval, and seizure freedom for >3 months at the time of death or last follow‐up were recorded.ResultsFive of 12 dogs were euthanized <12 months after implantation. All 7 remaining dogs showed >50% decrease in seizure frequency until last follow‐up, starting at a median of 37 to 48 months after implantation (range, 0‐6 to 61‐72 months) and a >3‐fold increase in mean inter‐seizure interval starting a median of 25 to 36 months after implantation (range, 0‐6 months to 49‐60 months), 3/7 dogs were seizure‐free at death or last follow‐up.Conclusions and Clinical ImportanceMonthly seizure frequencies decreased and inter‐seizure intervals increased in all dogs 2 to 3 years after VNS implantation, but a high proportion were euthanized before this time point. Prospective clinical trials are required to establish causality and the magnitude of this association.
Molecular mapping of virus‐infected cells with immunogold and metal‐tagging transmission electron microscopy
Authors: Sachse Martin , de Castro Isabel Fernández , Tenorio Raquel , Risco Cristina 
Published: October 2023

AbstractTransmission electron microscopy (TEM) has been essential to study virus–cell interactions. The architecture of viral replication factories, the principles of virus assembly and the components of virus egress pathways are known thanks to the contribution of TEM methods. Specially, when studying viruses in cells, methodologies for labeling proteins and other macromolecules are important tools to correlate morphology with function. In this review, we present the most widely used labeling method for TEM, immunogold, together with a lesser known technique, metal‐tagging transmission electron microscopy (METTEM) and how they can contribute to study viral infections. Immunogold uses the power of antibodies and electron dense, colloidal gold particles while METTEM uses metallothionein (MT), a metal‐binding protein as a clonable tag. MT molecules build gold nano‐clusters inside cells when these are incubated with gold salts. We describe the necessary controls to confirm that signals are specific, the advantages and limitations of both methods, and show some examples of immunogold and METTEM of cells infected with viruses.
Lipidome remodeling in response to nutrient replenishment requires the tRNA modifier Deg1/Pus3 in yeast
Authors: Matos Gabriel Soares , Vogt Leonie , Santos Rosangela Silva , Devillars Aurélien , Yoshinaga Marcos Yukio , Miyamoto Sayuri , Schaffrath Raffael , Montero‐Lomeli Monica , Klassen Roland 
Published: October 2023

AbstractIn the yeast Saccharomyces cerevisiae, the absence of the pseudouridine synthase Pus3/Deg1, which modifies tRNA positions 38 and 39, results in increased lipid droplet (LD) content and translational defects. In addition, starvation‐like transcriptome alterations and induced protein aggregation were observed. In this study, we show that the deg1 mutant increases specific misreading errors. This could lead to altered expression of the main regulators of neutral lipid synthesis which are the acetyl‐CoA carboxylase (Acc1), an enzyme that catalyzes a key step in fatty acid synthesis, and its regulator, the Snf1/AMPK kinase. We demonstrate that upregulation of the neutral lipid content of LD in the deg1 mutant is achieved by a mechanism operating in parallel to the known Snf1/AMPK kinase‐dependent phosphoregulation of Acc1. While in wild‐type cells removal of the regulatory phosphorylation site (Ser‐1157) in Acc1 results in strong upregulation of triacylglycerol (TG), but not steryl esters (SE), the deg1 mutation more specifically upregulates SE levels. In order to elucidate if other lipid species are affected, we compared the lipidomes of wild type and deg1 mutants, revealing multiple altered lipid species. In particular, in the exponential phase of growth, the deg1 mutant shows a reduction in the pool of phospholipids, indicating a compromised capacity to mobilize acyl‐CoA from storage lipids. We conclude that Deg1 plays a key role in the coordination of lipid storage and mobilization, which in turn influences lipid homeostasis. The lipidomic effects in the deg1 mutant may be indirect outcomes of the activation of various stress responses resulting from protein aggregation.
Evaluation of the deficiency status of 25‐hydroxyvitamin D and associated factors in Southwest China: A hospital‐based retrospective cross‐sectional analysis of a low‐latitude, high‐altitude, multiracial region
Authors: Chan Ying , Cai Dongling , Guo Rongfen , Zhou Xiaoyan , He Guangyu , Li Huiping , Geng Zibiao , Guo Yan , Lin Junyue , Wang Ruihong , Jiang Lihong , Zhu Baosheng 
Published: October 2023

AbstractVitamin D deficiency is widespread in different populations and regions worldwide and has become a global health issue. The vitamin D status of the population in the Yunnan Province of Southwest China has not been evaluated to date. Therefore, in this study, we evaluated the vitamin D status according to the serum concentrations of 25‐hydroxyvitamin D (25(OH)D) in individuals of Yunnan Province, a low‐latitude, high‐altitude and multiracial region in China. The data on 25(OH)D concentrations from October 2012 to December 2017 were retrospectively collected and assessed using the laboratory information system from 52 950 hospital‐based participants (age, 1 day–96 years; females, 73.74%). The serum concentration of 25(OH)D was evaluated using a chemiluminescent immunoassay. The analysis was stratified by sex, age, sampling season, testing year, minority, residential district, latitude, altitude and meteorological factors. Vitamin D status was classified as follows: severe deficiency: <10 ng/mL; deficiency: <20 ng/mL; insufficiency: <30 ng/mL; and sufficiency: ≥30 ng/mL. The results showed that vitamin D deficiency is highly prevalent in Yunnan Province in a hospital‐based cohort, with a deficiency and severe deficiency rate of 65.1% and a sufficiency rate of 5.30%. Significantly lower vitamin D levels and sufficiency rates were observed in females than in males (20.13 ± 7.22 ng/mL vs. 17.56 ± 6.66 ng/mL and 8.20% vs. 4.20%; p < 0.01, respectively); in spring and winter (16.93 ± 6.24 ng/mL; 2.97% and 16.38 ± 6.43 ng/mL; 3.06%, respectively) than in summer and autumn (20.23 ± 7.14 ng/mL; 8.02% and 19.10 ± 6.97 ng/mL; 6.61% [p < 0.01], respectively); and in older individuals (0–6 years: 28.29 ± 13.13 ng/mL vs. >60 years: 14.88 ± 8.39 ng/mL; p < 0.01). Relatively higher vitamin D levels were observed in individuals of Yi, Zhuang, Hani, Dai, Miao and Lisu minorities and lower levels in individuals of Hui and Zang minorities compared with those of the Han nationality (p < 0.01). The mean sunlight duration, mean air temperature, maximum ultraviolet value and latitude were significantly correlated with vitamin D levels (r = −0.53, 0.60, 0.31, −0.68, respectively; p < 0.05). These results suggest that vitamin D status is influenced by sex, age, minority, latitude and some meteorological factors in areas with high and low altitudes. Hence, new public health policies, such as advice on sunshine exposure, food fortification and nutrition education, as well as the implementation of vitamin D supplementation programmes must be considered to alleviate vitamin D deficiency in Yunnan province, Southwest China.
A pilot study of anti‐nephrin antibodies in podocytopaties among adults
Authors: Chebotareva Natalia , Vinogradov Anatoliy , Birukova Yevgeniya , Alentov Igor , Sergeeva Natalia , Chemodanova Daria , Kononikhin Alexey S. , Moiseev Sergey V. 
Published: October 2023

AbstractAimMinimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are podocytopathies characterized by damage to the glomerular filtration barrier, leading to proteinuria and nephrotic syndrome. The production of anti‐podocyte antibodies has been proposed as potential circulating factors contributing to the development of these conditions. The aim of the study is to evaluate the levels of anti‐nephrin antibodies in patients with podocytopathies and healthy subjects.MethodsIn this study, a total of 77 patients with active glomerulopathy and 11 healthy subjects were included. Forty one patients were diagnosed with FSGS, 11 with MCD, and 25 with MN. To measure the levels of anti‐nephrin antibodies, enzyme‐linked immunosorbent assay was used.ResultsThe levels of antibodies to nephrin were significantly higher in patients with MCD 61.2 [28.9–66.3] ng/mL and FSGS 32.5 [17.2–58.4] ng/mL compared to MN 20.3 [14.4–38.4] and healthy individuals 15.3 [12–18.9] ng/mL, p < .05. In patients with primary FSGS, the levels of antibodies to nephrin were significantly higher 45.2 [20–64.3] ng/mL compared to patients with secondary FSGS 26.7 [11.2–44.1] ng/mL, p < .05. There were no significant differences in the remission rate between the anti‐nephrin antibodies positive and negative groups (log‐rank test: p = .158).ConclusionThe level of anti‐nephrin antibodies was found to be significantly higher in patients with MCD and pFSGS compared to those with sFSGS, MN, and healthy subjects. Anti‐nephrin antibodies in MCD and primary FSGS may be associated with the severity of podocytopathies, however they did not have an impact on the response to therapy.
How likely is the sense‐B‐noise to affect patients with subcutaneous implantable cardioverter‐defibrillators and can we solve that problem in every case?
Authors: Budrejko Szymon , Zienciuk‐Krajka Agnieszka , Olędzki Szymon , Daniłowicz‐Szymanowicz Ludmiła , Kempa Maciej 
Published: October 2023

AbstractBackgroundSense‐B‐noise is a newly reported possible cause of inappropriate shocks in patients with subcutaneous implantable cardioverter‐defibrillators (S‐ICDs). The nature of that noise is unknown, it is not related to mechanical failure of the S‐ICD system. Reprogramming to the secondary sensing vector is suggested by the producer as a possible solution.MethodsWe analyzed the medical records of S‐ICD recipients from two university clinical centers (Gdansk and Szczecin, Poland). Our aim was to determine the rate of sense‐B‐noise, and whether the secondary sensing vector would be available for reprogramming if such a problem occurred in our patients.ResultsThe sense‐B‐noise issue affected three patients in our cohort (3%), which corresponds to the incidence of 0.012 events per patient‐year of follow‐up. The primary vector was permanently used in 47 patients (52%), secondary in 28 (31%), and alternate in 16 (17%), respectively. Therefore, the total number of patients potentially vulnerable to sense‐B noise (with the primary or alternate vector programmed permanently) was 63 (69%). Among those 63 patients, 51 individuals (81%) had also the secondary vector available for permanent use.ConclusionThe sense‐B‐noise affected 3% of patients in our cohort, with an incidence of 0.012 per patient‐year of follow‐up. Most patients potentially vulnerable to sense‐B noise could be reprogrammed to the secondary sensing vector, if necessary. Further investigation of the sense‐B‐noise issue is needed.
Cassava brown streak: A deadly virus on the move
Authors: Robson Fran , Hird Diane L. , Boa Eric 
Published: October 2023

AbstractThe beginning of the 21st century witnessed a vast and devastating upsurge in the incidence and spread of cassava brown streak ipomovirus disease (CBSD) in eastern countries of sub‐Saharan Africa that decimated crops and jeopardized food security in the region. The disease pandemic may have resulted from an increase in superabundant African whitefly populations, the insect vector, in the early 2000s, and the unintended dissemination of infected or highly disease‐susceptible cassava planting material during agricultural development and relief programmes. This plant pandemic report, one of a series of plant disease reports commissioned by the British Society for Plant Pathology, tells the story of CBSD, its history, its impact, the devastating storage root decay it triggers, and the efforts to understand the causal agent and mitigate its damaging effect on an important staple crop.
Patterns of multiple chronic conditions in pregnancy: Population‐based study using latent class analysis
Authors: Brown Hilary K. , Fung Kinwah , Cohen Eyal , Dennis Cindy‐Lee , Grandi Sonia M. , Rosella Laura C. , Varner Catherine , Vigod Simone N. , Wodchis Walter P. , Ray Joel G. 
Published: October 2023

AbstractBackgroundAdults with multiple chronic conditions (MCC) are a heterogeneous population with elevated risk of future adverse health outcomes. Yet, despite the increasing prevalence of MCC globally, data about MCC in pregnancy are scarce.ObjectivesTo estimate the population prevalence of MCC in pregnancy and determine whether certain types of chronic conditions cluster together among pregnant women with MCC.MethodsWe conducted a population‐based cohort study in Ontario, Canada, of all 15–55‐year‐old women with a recognised pregnancy, from 2007 to 2020. MCC was assessed from a list of 22 conditions, identified using validated algorithms. We estimated the prevalence of MCC. Next, we used latent class analysis to identify classes of co‐occurring chronic conditions in women with MCC, with model selection based on parsimony, clinical interpretability and statistical fit.ResultsAmong 2,014,508 pregnancies, 324,735 had MCC (161.2 per 1000, 95% confidence interval [CI] 160.6, 161.8). Latent class analysis resulted in a five‐class solution. In four classes, mood and anxiety disorders were prominent and clustered with one additional condition, as follows: Class 1 (22.4% of women with MCC), osteoarthritis; Class 2 (23.7%), obesity; Class 3 (15.8%), substance use disorders; and Class 4 (22.1%), asthma. In Class 5 (16.1%), four physical conditions clustered together: obesity, asthma, chronic hypertension and diabetes mellitus.ConclusionsMCC is common in pregnancy, with sub‐types dominated by co‐occurring mental and physical health conditions. These data show the importance of preconception and perinatal interventions, particularly integrated care strategies, to optimise treatment and stabilisation of chronic conditions in women with MCC.
Natural variation in a short region of the Acidovorax citrulli type III‐secreted effector AopW1 is associated with differences in cytotoxicity and host adaptation
Authors: Jiménez‐Guerrero Irene , Sonawane Monica , Eckshtain‐Levi Noam , Tuang Za Khai , da Silva Gustavo Mateus , Pérez‐Montaño Francisco , Leibman‐Markus Meirav , Gupta Rupali , Noda‐Garcia Lianet , Bar Maya , Burdman Saul 
Published: October 2023

SUMMARYBacterial fruit blotch, caused by Acidovorax citrulli, is a serious disease of melon and watermelon. The strains of the pathogen belong to two major genetic groups: group I strains are strongly associated with melon, while group II strains are more aggressive on watermelon. A. citrulli secretes many protein effectors to the host cell via the type III secretion system. Here we characterized AopW1, an effector that shares similarity to the actin cytoskeleton‐disrupting effector HopW1 of Pseudomonas syringae and with effectors from other plant‐pathogenic bacterial species. AopW1 has a highly variable region (HVR) within amino acid positions 147 to 192, showing 14 amino acid differences between group I and II variants. We show that group I AopW1 is more toxic to yeast and Nicotiana benthamiana cells than group II AopW1, having stronger actin filament disruption activity, and increased ability to induce cell death and reduce callose deposition. We further demonstrated the importance of some amino acid positions within the HVR for AopW1 cytotoxicity. Cellular analyses revealed that AopW1 also localizes to the endoplasmic reticulum, chloroplasts, and plant endosomes. We also show that overexpression of the endosome‐associated protein EHD1 attenuates AopW1‐induced cell death and increases defense responses. Finally, we show that sequence variation in AopW1 plays a significant role in the adaptation of group I and II strains to their preferred hosts, melon and watermelon, respectively. This study provides new insights into the HopW1 family of bacterial effectors and provides first evidence on the involvement of EHD1 in response to biotic stress.
Picky eaters: selective autophagy in plant cells
Authors: Cadena‐Ramos Alexis I. , De‐la‐Peña Clelia 
Published: October 2023

SUMMARYAutophagy, a fundamental cellular process, plays a vital role in maintaining cellular homeostasis by degrading damaged or unnecessary components. While selective autophagy has been extensively studied in animal cells, its significance in plant cells has only recently gained attention. In this review, we delve into the intriguing realm selective autophagy in plants, with specific focus on its involvement in nutrient recycling, organelle turnover, and stress response. Moreover, recent studies have unveiled the interesting interplay between selective autophagy and epigenetic mechanisms in plants, elucidating the significance of epigenetic regulation in modulating autophagy‐related gene expression and finely tuning the selective autophagy process in plants. By synthesizing existing knowledge, this review highlights the emerging field of selective autophagy in plant cells, emphasizing its pivotal role in maintaining nutrient homeostasis, facilitating cellular adaptation, and shedding light on the epigenetic regulation that governs these processes. Our comprehensive study provides the way for a deeper understanding of the dynamic control of cellular responses to nutrient availability and stress conditions, opening new avenues for future research in this field of autophagy in plant physiology.