bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adeno‐Associated Virus‐Encapsulated Alginate Microspheres Loaded in Collagen Gel Carriers for Localized Gene Transfer
Authors: Kurashina Yuta , Kurihara Sho , Kubota Takeshi , Takatsuka Shuhei , Hirabayashi Motoki , Shimmura Hajime , Miyahara Hideo , Hioki Aiki , Matsushita Yutaka , Muramatsu Jumpei , Ogawa Yuki , Fujioka Masato , Okano Hirotaka J. , Onoe Hiroaki 
Published: January 2024

AbstractThis work reports localized in vivo gene transfer by biodegradation of the adeno‐associated virus‐encapsulating alginate microspheres (AAV‐AMs) loaded in collagen gel carriers. AAV‐AMs are centrifugally synthesized by ejecting a mixed pre‐gel solution of alginate and AAV to CaCl2 solution to form an ionically cross‐linked hydrogel microsphere immediately. The AAV‐AMs are able to preserve the AAV without diffusing out even after spreading them on the cells, and the AAV is released and transfected by the degradation of the alginate microsphere. In addition, AAV‐AMs can be stored by cryopreservation until use. By implanting this highly convenient AAV‐encapsulated hydrogel, AAV‐AMs can be loaded into collagen gel carriers to fix the position of the implanted AAV‐AMs and achieve localized gene transfer in vivo. In vivo experiments show that the AAV‐AMs loaded in collagen gel carriers are demonstrated to release the encapsulated AAV for gene transfer in the buttocks muscles of mice. While conventional injections caused gene transfer to the entire surrounding tissue, the biodegradation of AAV‐AMs shows that gene transfer is achieved locally to the muscles. This means that the proposed AAV‐loaded system is shown to be a superior method for selective gene transfer.
Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)
Authors: Logan Natasha , Cao Cuong , Freitag Stephan , Haughey Simon A. , Krska Rudolf , Elliott Christopher T. 
Published: January 2024

AbstractThe implementation of low‐cost and rapid technologies for the on‐site detection of mycotoxin‐contaminated crops is a promising solution to address the growing concerns of the agri‐food industry. Recently, there have been significant developments in surface‐enhanced Raman spectroscopy (SERS) for the direct detection of mycotoxins in food and feed. This review provides an overview of the most recent advancements in the utilization of SERS through the successful fabrication of novel nanostructured materials. Various bottom‐up and top‐down approaches have demonstrated their potential in improving sensitivity, while many applications exploit the immobilization of recognition elements and molecular imprinted polymers (MIPs) to enhance specificity and reproducibility in complex matrices. Therefore, the design and fabrication of nanomaterials is of utmost importance and are presented herein. This paper uncovers that limited studies establish detection limits or conduct validation using naturally contaminated samples. One decade on, SERS is still lacking significant progress and there is a disconnect between the technology, the European regulatory limits, and the intended end‐user. Ongoing challenges and potential solutions are discussed including nanofabrication, molecular binders, and data analytics. Recommendations to assay design, portability, and substrate stability are made to help improve the potential and feasibility of SERS for future on‐site agri‐food applications.
High‐Surface Area Mesoporous Sc2O3 with Abundant Oxygen Vacancies as New and Advanced Electrocatalyst for Electrochemical Biomass Valorization
Authors: Wu Yufeng , Ma Liyao , Wu Junxiu , Song Minwei , Wang Changlong , Lu Jun 
Published: January 2024

AbstractScandium oxide (Sc2O3) is considered as omnipotent “Industrial Ajinomoto” and holds promise in catalytic applications. However, rarely little attention is paid to its electrochemistry. Here, the first nanocasting design of high‐surface area Sc2O3 with abundant oxygen vacancies (mesoporous VO‐Sc2O3) for efficient electrochemical biomass valorization is reported. In the case of the electro‐oxidation of 5‐hydroxymethylfurfural (HMF) to 2,5‐furandicarboxylic acid (FDCA), quantitative HMF conversion, high yield, and high faradic efficiency of FDCA via the hydroxymethylfurancarboxylic acid pathway are achieved by this advanced electrocatalyst. The beneficial effect of the VO on the electrocatalytic performance of the mesoporous VO‐Sc2O3 is revealed by the enhanced adsorption of reactants and the reduced energy barrier in the electrochemical process. The concerted design, in situ and ex situ experimental studies and theoretical calculations shown in this work should shed light on the rational elaboration of advanced electrocatalysts, and contribute to the establishment of a circular carbon economy since the bio‐plastic monomer and green hydrogen are efficiently synthesized.
Prodrug Celecoxib‐Derived Nanoparticles Potentiate the Efficacy of Cancer Immunotherapy by Remodeling the Tumor Microenvironment
Authors: Kim Tae Woo , Whang Chang‐Hee , Kim Dohyeon , Jon Sangyong 
Published: January 2024

AbstractCyclooxygenase‐2 (COX‐2) and prostaglandin E2 (PGE2) play crucial roles in promoting tumor growth and facilitating immune evasion within the tumor microenvironment (TME)—functions that limit responses to immunotherapy. Recent research has highlighted the potential of celecoxib (CXB), a potent COX‐2 selective inhibitor, for enhancing the effectiveness of immunotherapy by blocking the COX‐2/PGE2 axis. However, the clinical application of CXB for cancer treatment is still hindered by its systemic adverse effects and lack of tumor‐targeting. Here, to address these limitations, PEGylated prodrug CXB‐derived nanoparticles (CXB‐NPs) are developed, a nanomedicine designed to improve the tumor delivery of CXB while minimizing its adverse side effects. CXB‐NPs demonstrate enhanced tumor accumulation and effectively inhibit tumor growth by improving the immunosuppressive TME in immunocompetent mice, surpassing the performance of parental CXB. Furthermore, when combined with anti‐PD‐1 antibody (αPD‐1) immunotherapy, CXB‐NPs exhibit superior suppression of CT26 tumor growth compared with αPD‐1 monotherapy. This combination approach reduces the infiltration of immunosuppressive immune cells while promoting the infiltration and cytotoxicity of CD8+ T cells. The findings indicate that CXB‐NPs capable of remodeling the TME provide a new combination therapy strategy for potentiating antitumor efficacy.
Salt Effect Engineering Single Fe‐N2P2‐Cl Sites on Interlinked Porous Carbon Nanosheets for Superior Oxygen Reduction Reaction and Zn‐Air Batteries
Authors: Tan Xiaojie , Zhang Jinqiang , Cao Fengliang , Liu Yachao , Yang Hao , Zhou Qiang , Li Xudong , Wang Rui , Li Zhongtao , Hu Han , Zhao Qingshan , Wu Mingbo 
Published: January 2024

AbstractDeveloping efficient metal‐nitrogen‐carbon (M‐N‐C) single‐atom catalysts for oxygen reduction reaction (ORR) is significant for the widespread implementation of Zn‐air batteries, while the synergic design of the matrix microstructure and coordination environment of metal centers remains challenges. Herein, a novel salt effect‐induced strategy is proposed to engineer N and P coordinated atomically dispersed Fe atoms with extra‐axial Cl on interlinked porous carbon nanosheets, achieving a superior single‐atom Fe catalyst (denoted as Fe‐NP‐Cl‐C) for ORR and Zn‐air batteries. The hierarchical porous nanosheet architecture can provide rapid mass/electron transfer channels and facilitate the exposure of active sites. Experiments and density functional theory (DFT) calculations reveal the distinctive Fe‐N2P2‐Cl active sites afford significantly reduced energy barriers and promoted reaction kinetics for ORR. Consequently, the Fe‐NP‐Cl‐C catalyst exhibits distinguished ORR performance with a half‐wave potential (E1/2) of 0.92 V and excellent stability. Remarkably, the assembled Zn‐air battery based on Fe‐NP‐Cl‐C delivers an extremely high peak power density of 260 mW cm−2 and a large specific capacity of 812 mA h g−1, outperforming the commercial Pt/C and most reported congeneric catalysts. This study offers a new perspective on structural optimization and coordination engineering of single‐atom catalysts for efficient oxygen electrocatalysis and energy conversion devices.
KxVPO4F (x∼0): A New High‐Voltage and Low‐Stain Cathode Material for Ultrastable Calcium Rechargeable Batteries
Authors: Li Renjie , Lee Youngsu , Lin Huijun , Che Xiangli , Pu Xiangjun , Yi Yuyang , Chen Feiyang , Yu Jingya , Chan Kang Cheung , Park Kyu‐Young , Xu Zheng‐Long 
Published: January 2024

AbstractThe utilization of high‐voltage intercalation cathodes in calcium‐ion batteries (CIBs) is impeded by the substantial size and divalent character of Ca2+ ions, which result in pronounced volume alterations and sluggish ion mobility, consequently causing inferior reversibility and low energy/power densities. To tackle these issues, polyanionic K‐vacant KxVPO4F (x∼0, designated as K0VPF) is proposed as high‐voltage and ultra‐stable cathode material in CIBs. The K0VPF demonstrates a decent calcium storage capacity of 75 mAh g−1 at 10 mA g−1 and remarkable capacity retention of 84.2% over 1000 cycles. The average working voltage of the K0VPF is 3.85 V versus Ca2+/Ca, representing the highest value reported for CIB cathodes to date. The combined experimental and theoretical investigations revealed that the low volume changes and hopping diffusion barriers contribute to the extraordinary stability and high‐power capabilities, respectively, of K0VPF. The distribution of Ca ions into polyanionic frameworks with pronounced spatial separation effectively attenuates the Ca2+–Ca2+ repulsive force and thus augmenting the Ca migration kinetics. The high voltage of K0VPF is attributed to the inductive effect from the largely electronegative fluorine. In conjunction with a calcium metal anode and a compatible electrolyte, Ca metal full cells featured a record‐high energy density of ≈300 Wh kg−1.
Solid Polymer Electrolytes with Enhanced Electrochemical Stability for High‐Capacity Aluminum Batteries
Authors: Leung Oi Man , Gordon Leo W. , Messinger Robert J. , Prodromakis Themis , Wharton Julian A. , Ponce de León Carlos , Schoetz Theresa 
Published: January 2024

AbstractChloroaluminate ionic liquids are commonly used electrolytes in rechargeable aluminum batteries due to their ability to reversibly electrodeposit aluminum at room temperature. Progress in aluminum batteries is currently hindered by the limited electrochemical stability, corrosivity, and moisture sensitivity of these ionic liquids. Here, a solid polymer electrolyte based on 1‐ethyl‐3‐methylimidazolium chloride‐aluminum chloride, polyethylene oxide, and fumed silica is developed, exhibiting increased electrochemical stability over the ionic liquid while maintaining a high ionic conductivity of ≈13 mS cm−1. In aluminum–graphite cells, the solid polymer electrolytes enable charging to 2.8 V, achieving a maximum specific capacity of 194 mA h g−1 at 66 mA g−1. Long‐term cycling at 2.7 V showed a reversible capacity of 123 mA h g−1 at 360 mA g−1 and 98.4% coulombic efficiency after 1000 cycles. Solid‐state nuclear magnetic resonance spectroscopy measurements reveal the formation of five‐coordinate aluminum species that crosslink the polymer network to enable a high ionic liquid loading in the solid electrolyte. This study provides new insights into the molecular‐level design and understanding of polymer electrolytes for high‐capacity aluminum batteries with extended potential limits.
Progress on Bifunctional Carbon‐Based Electrocatalysts for Rechargeable Zinc–Air Batteries Based on Voltage Difference Performance
Authors: Song Yijian , Li Weijie , Zhang Kai , Han Chao , Pan Anqiang 
Published: January 2024

AbstractZinc–air batteries (ZABs) hold potential as clean, cost‐effective, and sustainable energy storage system for the next generation. However, the application of ZABs remains challenging because of their poor rechargeability and low efficiency . The design of efficient bifunctional catalysts toward oxygen reduction reaction (ORR) during discharging and the oxygen evolution reaction (OER) during charging is essential to developing rechargeable ZABs. Transition metal (TM)‐doped carbon (TM‐C) materials stand out from all the available bifunctional catalysts due to the excellent specific surface area, diverse morphological structures , and the multiple metal active sites formed after TM doping. This paper, therefore, focuses on the synthesis, electrochemical properties, and potential mechanism of TM‐C catalysts. To make a novelty and logical statement, the voltage difference (ΔE = Ei = 10 − E1/2) between the ORR/OER catalytic process is employed to categorize different TM‐C catalysts reported in recent years, which are divided into two groups: I (ΔE = 0.7 − 0.9 V) and II (ΔE = 0.5 − 0.7 V). The catalytic mechanisms of bifunctional catalysts are clarified. More ways and ideas for synthesizing high‐performance bifunctional TM‐C catalysts are also provided. Finally, the current problem and prospects of this group materials are presented.
Shoreline slope influences movements of larval lampreys over dewatered substrate
Authors: Liedtke Theresa L. , Harris Julianne E. , Gray Ann E. 
Published: January 2024

AbstractLarval lampreys are filter feeders that live for several years burrowed in fine sediments in freshwater streams. Stream side channels and edges, where larval lampreys gather, are vulnerable to natural and human‐caused dewatering. Water level reductions can strand and kill thousands of larval lampreys, in part because many remain burrowed until their habitats are exposed, at which point larvae must emerge and attempt to move over dewatered substrate to locate wetted habitat. Dewatering for restoration efforts or seasonal closures of irrigation canals can be done slowly to reduce lamprey strandings, but in some settings, mechanisms are lacking to control the dewatering rate. Phased dewatering, where water level is reduced in stages separated by periods of static water level, could provide options when dewatering rate cannot be tightly controlled. To guide this phased approach, information is needed on the movement capability of larval lampreys. We examined larval lamprey (Entosphenus tridentatus and Lampetra spp.) movement distance and rate over dewatered substrate at shoreline slopes of 1%, 5%, 10% and 20% in a laboratory setting and modelled results using gamma regression models. Model results suggest both movement distance and movement rate increased with increasing slope and increasing larval length. We used the models to predict minimum distances and rates that 90%, 75% and 50% of medium‐sized (75 mm) lampreys would move over dewatered substrates on slopes of 1%–20%. The models predicted that 50% of larvae could move distances of ≥31 cm at rates of ≥0.7 mm/s on a 1% slope and distances of ≥502 cm at rates of ≥8.6 mm/s on a 20% slope. We present an example scenario of how information on larval movement capabilities and shoreline slope could guide phased dewatering events to limit impacts to lampreys.
Control over the aggregated structure of donor–acceptor conjugated polymer films for high‐mobility organic field‐effect transistors
Authors: Cao Xinxiu , Han Yanchun 
Published: January 2024

AbstractDonor–acceptor (D‐A) conjugated polymers have demonstrated great potential in organic field‐effect transistors application, and their aggregated structure is a crucial factor for high charge mobility. However, the aggregated structure of D‐A conjugated polymer films is complex and the structure–property relationship is difficult to understand. This review provides an overview of recent progress in controlling the aggregated structure of D‐A conjugated polymer films for higher mobility, including the mechanisms, methods, and properties. We first discuss the multilevel microstructures of D‐A conjugated polymer films, and then summarize the current understanding of the relationship between film microstructures and charge transport properties. Subsequently, we review the theory of D‐A conjugated polymer crystallization. After that, we summarize the common methods to control the aggregated structure of semi‐crystalline and near‐amorphous D‐A conjugated polymer films, such as crystallites and aggregates, tie chains, film alignment, and attempt to understand them from the basic theory of polymer crystallization. Finally, we provide the current challenges in controlling the aggregated structure of D‐A conjugated polymer films and in understanding the structure–property relationship.
Multi‐Functional Formaldehyde‐Nitrate Batteries for Wastewater Refining, Electricity Generation, and Production of Ammonia and Formate
Authors: An Siying , Zhao Zhi‐Hao , Bu Jun , He Jiaxin , Ma Wenxiu , Lin Jin , Bai Rui , Shang Li , Zhang Jian 
Published: January 2024

AbstractAs bulky pollutants in industrial and agricultural wastewater, nitrate and formaldehyde pose serious threats to the human health and ecosystem. Current purification technologies including chemical and bio‐/photo‐/electro‐chemical methods, are generally high‐cost, time‐consuming, or energy‐intensive. Here, we report a novel formaldehyde‐nitrate battery by pairing anodic formaldehyde oxidation with cathodic nitrate reduction, which simultaneously enables wastewater purification, electricity generation, and the production of high‐value‐added ammonia and formate. As a result, the formaldehyde‐nitrate battery remarkably exhibits an open‐circuit voltage of 0.75 V, a peak power density of 3.38 mW cm−2 and the yield rates of 32.7 mg h−1 cm−2 for ammonia and 889.4 mg h−1 cm−2 for formate. In a large‐scale formaldehyde‐nitrate battery (25 cm2), 99.9 % of nitrate and 99.8 % of formaldehyde are removed from simulated industrial wastewater and the electricity of 2.03 W⋅h per day is generated. Moreover, the design of such a multi‐functional battery is universally applicable to the coupling of NO3− or NO2− reduction with various aldehyde oxidization, paving a new avenue for wastewater purification and chemical manufacturing.
Fractures in Patients With Acute Calcium Pyrophosphate Crystal Arthritis Versus Matched Comparators in a Large Cohort Study
Authors: Tedeschi Sara K. , Hayashi Keigo , Rosenthal Ann , Gill Muneet , Marrugo Javier , Fukui Sho , Gravallese Ellen , Solomon Daniel H. 
Published: January 2024

ObjectiveCalcium pyrophosphate deposition (CPPD) disease was associated with osteopenia in two cross‐sectional studies. We compared fracture risks in patients with acute calcium pyrophosphate (CPP) crystal arthritis versus matched comparators.MethodsWe performed a longitudinal cohort study using electronic health record data from a single large academic health system, with data from 1991 to 2023. Patients with one or more episodes of acute CPP crystal arthritis were matched to comparators on the index date (first documentation of “pseudogout” or synovial fluid CPP crystals or matched encounter) and first encounter in the health system. The primary outcome was first fracture at the humerus, wrist, hip, or pelvis. We excluded patients with fracture before the index date. Covariates included demographics, body mass index, smoking, comorbidities, health care use, glucocorticoids, and osteoporosis treatments. We estimated incidence rates and adjusted hazard ratios for fracture. Sensitivity analyses excluded patients prescribed glucocorticoids, patients prescribed osteoporosis treatments, or patients with rheumatoid arthritis and additionally adjusted for chronic kidney disease.ResultsWe identified 1,148 patients with acute CPP crystal arthritis matched to 3,730 comparators, with a mean age of 73 years. Glucocorticoids and osteoporosis treatments were more frequent in the acute CPP crystal arthritis cohort. Fracture incidence rates were twice as high in the acute CPP crystal arthritis cohort (11.7 per 1,000 person‐years) versus comparators (5.5 per 1,000 person‐years). After multivariable adjustment, fracture relative risk was twice as high in the acute CPP crystal arthritis cohort (hazard ratio 1.8 [95% confidence interval 1.3–2.3]); results were similar in sensitivity analyses.ConclusionIn this first published study of fractures and CPPD, fracture risk was nearly doubled in patients with acute CPP crystal arthritis.
Non‐metallic Cation Storage using Poly(3,4‐ethylenedioxythiophene) Shells
Authors: Rui Dou Xin , Yu Meng Qing , Zhong Chi Hong 
Published: January 2024

AbstractPrevious studies propose that NH4+ storage in metal oxides and metallic ion storage using Poly(3,4‐ethylenedioxythiophene) (PEDOT) both undergo diffusion‐controlled processes. This study, employing experimental and simulation studies, reveals that flower‐like PEDOT shells predominantly store NH4+ ions storage through surface‐controlled processes, constituting over 80 percent of the overall storage. NH4+ ions interact with PEDOT′s π–π stacking via van der Waals interactions, circumventing ionization energy and entropy barriers. Additionally, the controlled polymerization and crystallinity of PEDOT contribute significantly to this storage process. The reduction in lamellar packing distance and face‐to‐face distance between the molecular chains, coupled with an increase in crystalline domains, intensifies inter‐chain weak interactions and extends the overlap length of wave functions. These findings offer a comprehensive insight into cation storage using organic polymers.
How does digitization enable green innovation? Evidence from Chinese listed companies
Authors: Chen Wen , Zhu Changyi , Cheung Qi , Wu Siying , Zhang Jun , Cao Jia 
Published: January 2024

AbstractThe coordinated development of digitization and greening is an essential requirement for the sustainable development of the global economy. With the rapid development of the digital economy, enterprises are facing a new external environment for green innovation. This paper uses the robust principal component analysis method to construct the digital economy development index for Chinese prefecture‐level or above cities from 2012 to 2019 and examines the impact of the digital economy on corporate green innovation. It is found that the development of the digital economy can stimulate corporate green innovation by alleviating financing constraints, improving internal control, and reducing environmental uncertainty. This study further reveals that both increasing government environmental regulation and stronger judicial guarantees can enhance the positive impact of digitization. This research aims to provide inspiration and reference on how better to integrate digital economy development and corporate green innovation.
Unveiling PET Hydrolase Surface Dynamics through Fluorescence Microscopy
Authors: Rennison A. P. , Nousi A. , Westh P. , Marie R. , Møller M. S. 
Published: January 2024

AbstractPET hydrolases are an emerging class of enzymes that are being heavily researched for their use in bioprocessing polyethylene terephthalate (PET). While work has been done in studying the binding of PET oligomers to the active site of these enzymes, the dynamics of PET hydrolases binding to a bulk PET surface is an unexplored area. Here, methods were developed for total internal reflection fluorescence (TIRF) microscopy and fluorescence recovery after photobleaching (FRAP) microscopy to study the adsorption and desorption dynamics of these proteins onto a PET surface. TIRF microscopy was employed to measure both on and off rates of two of the most commonly studied PET hydrolases, PHL7 and LCC, on a PET surface. It was found that these proteins have a much slower off rates on the order of 10−3 s−1, comparable to non‐productive binding in enzymes such as cellulose. In combination with FRAP microscopy, a dynamic model is proposed in which adsorption and desorption dominates over lateral diffusion over the surface. The results of this study could have implications for the future engineering of PET hydrolases, either to target them to a PET surface or to modulate interaction with their substrate.