bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of Village Budget Plan Selection Based on Priorities Using Method Promethee and Borda
Authors: Laily Dwi Yanti , Dedi Irawan Muhammad 
Published: October 2023

The rapid information technology that is happening now
has a significant impact on human life, including various
activities that occur at the village office. The village head
is responsible for managing funds at the village level
including the collection and accountability of funds in
accordance with the provisions of law no.33 of 2004
concerning the balance of central and regional
governments. This study aims to implement a decision
support system by combining two methods, namely the
promethee and borda methods in selecting village budget
plans in Tegal Sari village based on priority. The method
used in this research is the Research and Development
(R&D) method. The method used for calculations is the
PROMETHEE and Borda methods. The PROMETHEE
method is used to manage individual decisions from each
decision maker, while the Borda method is used to
manage group decisions resulting from the PROMETHEE
ranking method. The criteria in this study are Cost,
Completion Time, Impact on Regions/Society, Profits
from Sustainable Investment, Factors of Interest. The
results obtained from this research are Pemb. Health
Sarpras as an alternative priority for the village budget.
IDEA TESTING AND ANALOGY PREDICT CREATIVITY OF BIOLOGY STUDENTS IN ONLINE LEARNING
Authors: Diki  , Vi Sara Dem , Yuliastuti Endah Sulistyawati Eko , Dwisatyadini Mutimanda 
Published: October 2023

Identifying factors affecting students’ success is important. This is a study on creativity of biology students to solve their learning problem. The participants are biology students at Universitas Terbuka (UT), the only single mode distance learning university in Indonesia. conducting online tutorial in introductory biology. Descriptive and bivariate correlation statistics data are collected, including one dependent variable about improvement of problem-solving skill and six independent variables. The six independent variables are problem identification, information searching, idea generation, analogy, integrating different concepts, and idea testing. Demographic data are age, gender, and length of daily online activities. There are 73 students returned the survey with 23.4% response rate. Most students (more than 60%) agree to some extent in all the variables. There are different levels of agreement about the use of online tutorial regarding gender, age and length of daily online activities. There are only two out of six independent variables that have significant correlation (0.05 significance level, two-tailed) with improvement of problem-solving skill, which are analogy and idea testing.
Keywords: creativity, biology, problem-solving, distance learning, online learning
The effect of Toll-like receptor agonists on the immunogenicity of MVA-SARS-2-S vaccine after intranasal administration in mice
Authors: Do Kim Thi Hoang , Willenzon Stefanie , Ristenpart Jasmin , Janssen Anika , Volz Asisa , Sutter Gerd , Förster Reinhold , Bošnjak Berislav 
Published: October 2023

Background and aimsModified Vaccinia virus Ankara (MVA) represents a promising vaccine vector for respiratory administration to induce protective lung immunity including tertiary lymphoid structure, the bronchus-associated lymphoid tissue (BALT). However, MVA expressing the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein (MVA-SARS-2-S) required prime-boost administration to induce high titers of anti-Spike antibodies in serum and bronchoalveolar lavage (BAL). As the addition of adjuvants enables efficient tailoring of the immune responses even to live vaccines, we tested whether Toll-like receptor (TLR)-agonists affect immune responses induced by a single dose of intranasally applied MVA-SARS-2-S.MethodsWe intranasally immunized C57BL/6 mice with MVA-SARS-2-S vaccine in the presence of either TLR3 agonist polyinosinic polycytidylic acid [poly(I:C)], TLR4 agonist bacterial lipopolysaccharide (LPS) from Escherichia coli, or TLR9 agonist CpG oligodeoxynucleotide (CpG ODN) 1826. At different time-points after immunization, we analyzed induced immune responses using flow cytometry, immunofluorescent microscopy, and ELISA.ResultsTLR agonists had profound effects on MVA-SARS-2-S-induced immune responses. At day 1 post intranasal application, the TLR4 agonist significantly affected MVA-induced activation of dendritic cells (DCs) within the draining bronchial lymph nodes, increasing the ratio of CD11b+CD86+ to CD103+CD86+ DCs. Nevertheless, the number of Spike-specific CD8+ T cells within the lungs at day 12 after vaccination was increased in mice that received MVA-SARS-2-S co-administered with TLR3 but not TLR4 agonists. TLR9 agonist did neither significantly affect MVA-induced DC activation nor the induction of Spike-specific CD8+ T cells but reduced both number and size of bronchus-associated lymphoid tissue. Surprisingly, the addition of all TLR agonists failed to boost the levels of Spike-specific antibodies in serum and bronchoalveolar lavage.ConclusionsOur study indicates a potential role of TLR-agonists as a tool to modulate immune responses to live vector vaccines. Particularly TLR3 agonists hold a promise to potentiate MVA-induced cellular immune responses. On the other hand, additional research is necessary to identify optimal combinations of agonists that could enhance MVA-induced humoral responses.
Epicardial fat volume, an independent risk factor for major adverse cardiovascular events, had an incremental prognostic value to myocardial perfusion imaging in Chinese populations with suspected or known coronary artery disease with a normal left ventricular ejection fraction
Authors: Yang Le , Yu Wenji , Wan Peng , Wang JingWen , Shao Xiaoliang , Zhang Feifei , Yang Xiaoyu , Chen Yongjun , Li Qi , Jiang Dan , Wang Yufeng , Jiang Qi , Wang Jianfeng , Wang Yuetao 
Published: October 2023

BackgroundMost coronary artery disease (CAD) patients with a normal left ventricular ejection fraction (LVEF) experience a poor prognosis. Single-photon emission computerized tomography (SPECT)–myocardial perfusion imaging (MPI), a routine examination, is useful in assessing risk and predicting major adverse cardiovascular events (MACEs) in populations with suspected or known CAD. SPECT/CT is a “one-stop shop” examination, which, through non-contrast CT, can produce attenuation correction for MPI and obtain information on coronary artery calcium (CAC) and epicardial fat volume (EFV) simultaneously. This study aims to investigate the predictive and incremental value of EFV to MPI for MACE in Chinese populations with suspected or known CAD with a normal LVEF.Methods and resultsWe retrospectively studied 290 suspected or known CAD inpatients with a normal LVEF who underwent SPECT/CT between February 2014 and December 2017. Abnormal MPI was defined as a summed stress score ≥4 or summed difference score ≥2. EFV and CAC were calculated using non-contrast CT. The end date of follow-ups was in February 2022. The follow-up information was obtained from the clinical case notes of the patients or reviews of telephone calls. MACE was defined as cardiac death, late coronary revascularization ≥3 months after MPI, non-fatal myocardial infarction, angina-related rehospitalization, heart failure, and stroke. During the 76-month follow-up, the event rate was 32.0% (93/290). Univariate and multivariate Cox regression analyses concluded that high EFV (&gt;108.3 cm3) [hazard ratio (HR): 3.3, 95% CI: 2.1–5.2, P &lt; 0.000] and abnormal MPI (HR: 1.8, 95% CI: 1.1–2.8, P = 0.010) were independent risk factors for MACE. The event-free survival of patients with high EFV was significantly lower than that of the low EFV group (log-rank test P &lt; 0.001). In the subgroup with normal MPI, high EFV was associated with reduced event-free survival (log-rank P &lt; 0.01), with a higher annualized event rate (8.3% vs. 1.9%). Adding high EFV to MPI could predict MACEs more effectively, with a higher concordance index (0.56–0.69, P &lt; 0.01), higher global chi square (7.2–41.4, P &lt; 0.01), positive integrated discrimination improvement (0.10, P &lt; 0.01), and net reclassification index (0.37, P &lt; 0.01).ConclusionsIn Chinese populations with suspected or known CAD with normal LVEF, high EFV was an independent risk factor for MACE after adjusting for traditional risk factors, CAC and MPI. In subgroups with normal MPI, EFV could also improve risk stratification. Adding EFV to MPI had an incremental value for predicting MACE.
Association of serum 25-hydroxyvitamin D concentrations with all-cause mortality among individuals with kidney stone disease: the NHANES database prospective cohort study
Authors: Gao Meng , Liu Minghui , Chen Jinbo , Zhu Zewu , Chen Hequn 
Published: October 2023

BackgroundThe purpose of this study was to investigate the correlation between serum 25(OH)D concentrations and all-cause mortality in patients with kidney stone disease (KSD) as the effects of a deficiency in 25-hydroxyvitamin D on KSD patients are currently unclear.MethodsFor our prospective cohort study, we included 2,916 participants from the National Health and Nutrition Examination Survey (NHANES) 2007-2018. The National Death Index (NDI) was utilized to identify all causes of death and cause-specific mortality until December 31, 2018. We calculated hazard ratios (HR) and 95% confidence intervals (CIs) using multivariate Cox regression models.ResultsDuring the 18,859 person-years of follow-up, a total of 375 fatalities occurred, including 83 deaths from cardiovascular disease (CVD) and 79 deaths from cancer. At baseline, individuals with higher blood 25(OH)D concentrations had lower levels of glucose, glycohemoglobin, CRP, and insulin, as well as higher levels of HDL cholesterol (P &lt; 0.01). In the fully adjusted model (Model 3), compared to the group with the lowest 25(OH)D concentrations, those with serum 25(OH)D concentrations ≥75 nmol/L had hazard ratios (HRs) and 95% confidence intervals (CIs) of 0.48 (0.26, 0.87) for all-cause mortality (P=0.02, P for trend = 0.02). The association between serum 25(OH)D concentrations and all-cause mortality in KSD patients was found to be significantly non-linear. A 7% decrease in the risk of death from all causes was observed for each unit-nmol/L increase in serum 25(OH)D concentrations when the concentrations were below 27.7 nmol/L (P &lt; 0.05).ConclusionBased on the findings, KSD patients with insufficient serum 25(OH)D concentrations were at a higher risk of all-cause mortality. Therefore, it is crucial to maintain sufficient blood 25(OH)D concentrations and prevent 25(OH)D insufficiency in order to extend the lifespan of KSD patients.
The published trend of studies on COVID-19 and diabetes: bibliometric analysis
Authors: Li Yuanyuan , Peng Lei , Gu Wei 
Published: October 2023

BackgroundSince the COVID-19 pandemic outbreak, diabetes mellitus (DM) has been at the core of the confirmed risk factors for fatal or critical care unit-treated COVID-19 and COVID-19 related complications. Although relevant studies on DM have developed rapidly during the COVID-19 pandemic. However, the aforementioned research results have not been systematically quantified by means of bibliometric analysis.PurposeThe purpose of this study is to provide a comprehensive analysis of the current status and trends of publications related to DM research during the COVID19 epidemic.MethodsA bibliometric analysis was performed using the Web of Science database. In this study, we used citespace, R software and R-Bibliometrix to analyze keywords, most-cited authors, most-cited countries, most-cited global documents, and co-occurrence and co-citation networks.ResultsA total of 1688 publications was included in this study. Investigators from the United States contributed the most publications. The United States, China and Europe have the most collaboration with the other countries/regions. A total of 3355 institutions made contributions to this study. Of the top 10 institutions with the most publications, N8 Research Partnership showed the most centrality. Among the top 10 journals, Diabetes Research and Clinical Practice published the most articles. Among authors included, Khunti Kamlesh is rated first with 27 papers and has the highest centrality. The most frequently co-cited article is entitled “Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study”. The most popular keywords included diabetes, mortality, diabetes, outcome, occurrences, risk, and type 1 diabetes.ConclusionThis bibliometric study provides an overall picture of DM research and research trends during the COVID-19 pandemic and provides a basis for researchers to develop their next research strategies.
Design-based system performance assessment of a combined power and freshwater cogeneration system
Authors: Elfizon Elfizon , Nuñez Alvarez José Ricardo , Chammam Abdeljelil , Al-Kharsan Ibrahim H. , Jweeg Muhsin J. , Yánez-Moretta Patricio , Alayi Reza , Khan Imran , Byun Yung-Cheol , Madsen Dag Øivind 
Published: October 2023

In this research, the design and use of combined systems for the simultaneous production of water, heat, and energy have been proposed, and, to fulfill the water, electricity, and heat demands of a hotel, modeling of the multi-effect evaporative desalination (MED) and combined heat and power (CHP) generation system was done. Then, the design of these two systems was administered in a combined way. This design was applied in order to evaluate the economy of the combined system compared to separate systems. The performed scenario was executed every 24 h during the two seasons of the year. The genetic algorithm was used to optimize this system, and it was considered the objective function to minimize the annual costs. The results showed that the nominal capacity of the gas turbine and backup boiler in the CHP + MED + thermal energy storage (TES) system was (14%) larger and (8.2%) smaller, respectively, compared to the CHP+ MED system. In addition, by using the energy storage tank in the combined CHP + MED system, 5.1% of the annual costs were reduced.
Time to initiation of antenatal care visit and its predictors among reproductive age women in Ethiopia: Gompertz inverse Gaussian shared frailty model
Authors: Belay Daniel Gashaneh , Alemu Melaku Birhanu , Aragaw Fantu Mamo , Asratie Melaku Hunie 
Published: October 2023

BackgroundEarly initiation of antenatal care (ANC) is essential for the early detection of pregnancy-related problems and unfavorable pregnancy outcomes. However, a significant number of mothers do not initiate ANC at the recommended time. Therefore, this study aimed to determine the median time of ANC initiation and its predictors among reproductive-age women in Ethiopia.MethodsWe used the Ethiopian Demographic and Health Survey (EDHS) 2016 data set. The proportional hazard assumption was assessed using Schoenfeld residual test and log–log plot. A life table was used to determine the median survival time (time of ANC initiation). The Gompertz inverse Gaussian shared frailty model was the best-fitting model for identifying the predictors for the early initiation of ANC booking. Finally, the adjusted hazard ratio (AHR) with a 95% confidence interval (CI) was used to determine the significance of predictors.ResultsA total of 7,501 reproductive-aged women gave recent birth in the last 5 years preceding the survey. Nearly three in five women [61.95% (95% CI: 60.85–63.04%)] booked their first ANC visit with a median time of 4.4 months. Women who attended primary education (AHR = 1.10, 95% CI: 1.01–1.20), secondary and above (AHR = 1.26, 95% CI: 1.11–1.44), media exposure (AHR = 1.07, 95% CI: 1.00–1.16), rich wealthy (AHR = 1.17, 95% CI: 1.06–1.30), grand multiparous (AHR = 0.82, 95% CI: 0.72–0.93), unwanted pregnancy (AHR = 0.88, 95% CI: 0.81–0.96), small periphery region (AHR = 0.58, 95% CI: 0.51–0.67), and rural residence (AHR = 0.86, 95% CI: 0.75–0.99) were significantly associated with first ANC visit.ConclusionAccording to this study, a significant number of women missed their first ANC visit. The education status of women, place of residence, region, wealth index, media exposure, unintended pregnancy, and multi-parity were significantly associated with the time of initiation of the first ANC visit. Therefore, policymakers should focus on improving the socioeconomic status (education, media coverage, and wealth) of reproductive-aged women by prioritizing women who live in small periphery regions and rural residences to improve the early initiation of ANC.
Whole-genome bisulfite sequencing reveals the function of DNA methylation in the allotransplantation immunity of pearl oysters
Authors: Gu Zefeng , Yang Jingmiao , Lu Jinzhao , Yang Min , Deng Yuewen , Jiao Yu 
Published: October 2023

IntroductionIn the pearl culture industry, a major challenge is the overactive immunological response in pearl oysters resulting from allotransplantation, leading to shell-bead rejection and death. To better understand the molecular mechanisms of postoperative recovery and the regulatory role of DNA methylation in gene expression, we analyzed the changes in DNA methylation levels after allotransplantation in pearl oyster Pinctada fucata martensii, and elucidated the regulatory function of DNA methylation in promoter activity of nicotinic acetylcholine receptor (nAChR) gene.MethodsWe constructed nine DNA methylomes at different time points after allotransplantation and used bisulfite genomic sequencing PCR technology (BSP) to verify the methylation status in the promoter of nAChR. We performed Dual luciferase assays to determine the effect of the dense methylation region in the promoter on transcriptional activity and used DNA pull-down and mass spectrometry analysis to assess the capability of transcription factor binding with the dense methylation region.ResultThe DNA methylomes reveal that CG-type methylation is predominant, with a trend opposite to non-CG-type methylation. Promoters, particularly CpG island-rich regions, were less frequently methylated than gene function elements. We identified 5,679 to 7,945 differentially methylated genes (DMGs) in the gene body, and 2,146 to 3,385 DMGs in the promoter at each time point compared to the pre-grafting group. Gene ontology and pathway enrichment analyses showed that these DMGs were mainly associated with “cellular process”, “Membrane”, “Epstein-Barr virus infection”, “Notch signaling pathway”, “Fanconi anemia pathway”, and “Nucleotide excision repair”. Our study also found that the DNA methylation patterns of the promoter region of nAChR gene were consistent with the DNA methylomics data. We further demonstrated that the dense methylation region in the promoter of nAChR affects transcriptional activity, and that the methylation status in the promoter modulates the binding of different transcription factors, particularly transcriptional repressors.ConclusionThese findings enhance our understanding of the immune response and regulation mechanism induced by DNA methylation in pearl oysters after allotransplantation.
Immunosuppressive M2 TAMs represent a promising target population to enhance phagocytosis of ovarian cancer cells in vitro
Authors: Brauneck Franziska , Oliveira-Ferrer Leticia , Muschhammer Jana , Sturmheit Tabea , Ackermann Christin , Haag Friedrich , Schulze zur Wiesch Julian , Ding Yi , Qi Minyue , Hell Louisa , Schmalfeldt Barbara , Bokemeyer Carsten , Fiedler Walter , Wellbrock Jasmin 
Published: October 2023

IntroductionTumor-associated macrophages (TAMs) represent an important cell population within the tumor microenvironment, but little is known about the phenotype and function of these cells. The present study aims to characterize macrophages in high-grade serous ovarian cancer (HGSOC).MethodsPhenotype and expression of co-regulatory markers were assessed on TAMs derived from malignant ascites (MA) or peripheral blood (PB) by multiparametric flow cytometry. Samples were obtained from HGSOC patients (n=29) and healthy donors (HDs, n=16). Additional expression analysis was performed by RNAseq (n=192). Correlation with clinically relevant parameters was conducted and validated by a second patient cohort (n=517). Finally, the role of TIGIT in repolarization and phagocytosis was investigated in vitro.ResultsExpression of the M2-associated receptors CD163, CD204, and CD206, as well as of the co-regulatory receptors TIGIT, CD226, TIM-3, and LAG-3 was significantly more frequent on macrophages in HGSOC than in HDs. CD39 and CD73 were broadly expressed on (mainly M2) macrophages, but without a clear clustering in HGSOC. CD163 mRNA levels were higher in TAMs from patients with residual tumor mass after surgery and associated with a shorter overall survival. In addition, TIGIT expression was associated with a higher tumor grading, indicating a prognostic relevance of M2 infiltration in HGSOC. TIGIT blockade significantly reduced the frequency of M2 macrophages. Moreover, combined blockade of TIGIT and CD47 significantly increased phagocytosis of ovarian cancer cells by TAMs in comparison to a single blockade of CD47.ConclusionCombined blockade of TIGIT and CD47 represents a promising approach to enhance anti-CD47-facilitated phagocytosis.
The entanglement of extracellular matrix molecules and immune checkpoint inhibitors in cancer: a systematic review of the literature
Authors: Fejza Albina , Carobolante Greta , Poletto Evelina , Camicia Lucrezia , Schinello Giorgia , Di Siena Emanuele , Ricci Giuseppe , Mongiat Maurizio , Andreuzzi Eva 
Published: October 2023

IntroductionImmune-checkpoint inhibitors (ICIs) have emerged as a core pillar of cancer therapy as single agents or in combination regimens both in adults and children. Unfortunately, ICIs provide a long-lasting therapeutic effect in only one third of the patients. Thus, the search for predictive biomarkers of responsiveness to ICIs remains an urgent clinical need. The efficacy of ICIs treatments is strongly affected not only by the specific characteristics of cancer cells and the levels of immune checkpoint ligands, but also by other components of the tumor microenvironment, among which the extracellular matrix (ECM) is emerging as key player. With the aim to comprehensively describe the relation between ECM and ICIs’ efficacy in cancer patients, the present review systematically evaluated the current literature regarding ECM remodeling in association with immunotherapeutic approaches.MethodsThis review followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines and was registered at the International Prospective Register of Systematic Reviews (PROSPERO, CRD42022351180). PubMed, Web of Science, and Scopus databases were comprehensively searched from inception to January 2023. Titles, abstracts and full text screening was performed to exclude non eligible articles. The risk of bias was assessed using the QUADAS-2 tool.ResultsAfter employing relevant MeSH and key terms, we identified a total of 5070 studies. Among them, 2540 duplicates, 1521 reviews or commentaries were found and excluded. Following title and abstract screening, the full text was analyzed, and 47 studies meeting the eligibility criteria were retained. The studies included in this systematic review comprehensively recapitulate the latest observations associating changes of the ECM composition following remodeling with the traits of the tumor immune cell infiltration. The present study provides for the first time a broad view of the tight association between ECM molecules and ICIs efficacy in different tumor types, highlighting the importance of ECM-derived proteolytic products as promising liquid biopsy-based biomarkers to predict the efficacy of ICIs.ConclusionECM remodeling has an important impact on the immune traits of different tumor types. Increasing evidence pinpoint at ECM-derived molecules as putative biomarkers to identify the patients that would most likely benefit from ICIs treatments.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022351180, identifier CRD42022351180.