bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex change every year: A unique reproductive strategy of the stony coral,Fimbriaphyllia(Euphyllia)ancora
Authors: Shikina Shinya , Tsai Pin-Hsuan , Chiu Yi-Ling , Chang Ching-Fong 
Published: December 2023

AbstractThe present study documents a unique reproductive strategy of the colonial stony coral,Fimbriaphyllia ancora,during observations spanning 8 years. Of 26 colonies monitored at Nanwan Bay, southern Taiwan, about 70% changed their sexes every year, i.e., colonies that were males two years ago became females last year, and changed back to males this year. Apparently, the remaining 30% were permanently male or female. Sex-change and non-sex-change colonies were growing in close proximity or even side-by-side, suggesting that this sex change phenomenon is not driven by environmental factors. No significant differences were found in colony size between sex-change and non-sex-change colonies, suggesting that the sex change strategy may be related to intrinsic factors, e.g., age or genetics. Histological analysis showed that female-to-male sex change occurs 4-5 months after spawning, whereas male-to-female sex change occurs 0-3 months after sperm release. We propose that this unique strategy may increase success of sexual reproduction of sessile, colonial corals.
Computationally guided insights into substrate recognition and specificity of Alg1
Authors: Yu Cheng , Long Fangyuan , Li Ruijie , Ding Yi 
Published: December 2023

AbstractThe assembly of N-linked glycans for the N-glycosylation, which is an important protein modification process, starts with the consecutive synthesis of the dolichol-linked oligosaccharide (DLO) precursor in the endoplasmic reticulum (ER). Asparagine-linked glycosylation protein 1 (Alg1) is the first mannosyltransferase (MTase) in DLO synthesis, which transfers a mannose residue from the sugar donor GDP-mannose (GDP-Man) to the substrate dolichol pyrophosphate-GlcNAc2 (Dol-PP-GlcNAc2) to form a β-1,4 glycosidic bond. However, little is known about its process of substrate recognition and the mechanism for its substrate specificity. Here, the models of large-scale systems in solution and membrane environments were built, revealing the substrate recognition and specificity mechanism of Alg1 based on extensive all-atom microsecond molecular dynamics (MD) simulations. In this study, the unique non-natural donor recognition ofScAlg1 and its mechanisms was reported. Further, the cooperation of the hydrophobic region and lysine in substrate recognition was revealed for bothScAlg1 andHsAlg1. Moreover, the functional roles of these sites were confirmed via mutant activity assays invitro. Additionally, the substrate binding process of Alg1 was simulated, elucidating the effect of dolichol on substrate specificity. This work reports the substrate recognition of Alg1 and reveals mechanisms for its sugar donor and substrate selectivity, which could further aid in mechanistic studies of the DLO biosynthetic process and drug development targeting Alg1.
Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors inDrosophila
Authors: Bonanno Shivan L. , Sanfilippo Piero , Eamani Aditya , Sampson Maureen M. , Binu Kandagedon , Li Kenneth , Burns Giselle D. , Makar Marylyn E. , Zipursky S. Lawrence , Krantz David E. 
Published: December 2023

AbstractTo visualize the cellular and subcellular localization of neuromodulatory G-protein coupled receptors (GPCRs) inDrosophila, we implement a molecular strategy recently used to add epitope tags to ionotropic receptors at their endogenous loci. Leveraging evolutionary conservation to identify sites more likely to permit insertion of a tag, we generated constitutive and conditional tagged alleles forDrosophila5-HT1A, 5-HT2A, 5-HT2B, Octβ1R, Octβ2R, two isoforms of OAMB, and mGluR. The conditional alleles allow for the restricted expression of tagged receptor in specific cell types, an option not available for any previous reagents to label these proteins. We show that 5-HT1A and 5-HT2B localize to the mushroom bodies and central complex respectively, as predicted by their roles in sleep. By contrast, the unexpected enrichment of Octβ1R in the central complex and of 5-HT1A and 5-HT2A to nerve terminals in lobular columnar cells in the visual system suggest new hypotheses about their function at these sites. Using an additional tagged allele of the serotonin transporter, a marker of serotonergic tracts, we demonstrate diverse spatial relationships between postsynaptic 5-HT receptors and presynaptic 5-HT neurons, consistent with the importance of both synaptic and volume transmission. Finally, we use the conditional allele of 5-HT1A to show that it localizes to distinct sites within the mushroom bodies as both a postsynaptic receptor in Kenyon cells and a presynaptic autoreceptor.Significance StatementInDrosophila, despite remarkable advances in both connectomic and genomic studies, antibodies to many aminergic GPCRs are not available. We have overcome this obstacle using evolutionary conservation to identify loci in GPCRs amenable to epitope-tagging, and CRISPR/Cas9 genome editing to generated eight novel lines. This method also may be applied to other GPCRs and allows cell-specific expression of the tagged locus. We have used the tagged alleles we generated to address several questions that remain poorly understood. These include the relationship between pre- and post-synaptic sites that express the same receptor, and the use of relatively distant targets by pre-synaptic release sites that may employ volume transmission as well as standard synaptic signaling.
Homeostatic Dysregulation of Systemic CD8+T Cell Compartment in Lung Cancer Patients
Authors: Lee Sung-Woo , Yun Ju Sik , Kim Young Ju , Kim Hee-Ok , Cho Hyun-Ju , Park Cheol-Kyu , Oh In-Jae , Cho Jae-Ho 
Published: December 2023

AbstractCancer adapts various resistance mechanisms to counteract CD8+T cell attacks. While this suppression of antigen-specific CD8+T cells is common within the tumor microenvironment, little is known about how tumors affect CD8+T cells systemically. Here we show a new link between tumor-associated homeostatic dysregulation and uncontrolled differentiation of peripheral blood CD8+T cells. These CD8+T cells exhibited progressive alterations indicative of diminished quiescence, increased spontaneous activation, and more-differentiated proliferation-incompetent effector cells. This phenomenon was not limited to tumor-reactive cells but broadly applicable to non-specific cells, correlating with poor clinical responses to immune checkpoint inhibitor therapy. These findings provide a new mechanism by which cancer impairs CD8+T cells by dysregulating the homeostasis of systemic CD8+T cell populations.One-Sentence SummaryCancer-associated homeostatic dysregulation accelerates uncontrolled differentiation of systemic CD8+T cells.
Pharmacological modulation of dopamine receptors reveals distinct brain-wide networks associated with learning and motivation in non-human primates
Authors: Fujimoto Atsushi , Elorette Catherine , Fujimoto Satoka H. , Fleysher Lazar , Rudebeck Peter H. , Russ Brian E. 
Published: December 2023

AbstractThe neurotransmitter dopamine (DA) has a multifaceted role in healthy and disordered brains through its action on multiple subtypes of dopaminergic receptors. How modulation of these receptors influences learning and motivation by altering intrinsic brain-wide networks remains unclear. Here we performed parallel behavioral and resting-state functional MRI experiments after administration of two different DA receptor antagonists in macaque monkeys. Systemic administration of SCH-23390 (D1 antagonist) slowed probabilistic learning when subjects had to learn new stimulus-reward associations and diminished functional connectivity (FC) in cortico-cortical and fronto-striatal connections. By contrast, haloperidol (D2 antagonist) improved learning and broadly enhanced FC in cortical connections. Further comparisons between the effect of SCH-23390/haloperidol on behavioral and resting-state FC revealed specific cortical and subcortical networks associated with the cognitive and motivational effects of DA manipulation, respectively. Thus, we reveal distinct brain-wide networks that are associated with the dopaminergic control of learning and motivation via DA receptors.Significance StatementD1 and D2 receptors are heavily implicated in cognitive and motivational processes, as well as in a number of psychiatric disorders. Despite this, little is known about how selective manipulation of these different receptors impacts cognition through changing activity across brain-wide intrinsic networks. Here, we examined the acute behavioral and brain-wide effects of D1 and D2 receptor-selective antagonists, SCH-23390 and haloperidol, in macaques performing a probabilistic learning task. SCH administration diminished, and haloperidol improved, animals’ task performance. Mirroring these effects on behavior, SCH reduced, and haloperidol increased, the resting-state functional connectivity across brain-wide networks, most notably in the cortico-striatal areas. Thus, our results highlight the opposing effects of D1 and D2 receptor modulation on the brain and behavior.
Sorted stem/progenitor epithelial cells of pubertal bovine mammary gland present limited potential to reconstitute an organised mammary epithelium after transplantation
Authors: Finot Laurence , Hue-Beauvais Cathy , Aujean Etienne , Provost Fabienne Le , Chanat Eric 
Published: December 2023

AbstractThe development and maintenance of mammary gland tissue depend on the proliferation and differentiation of mammary stem and progenitor cells. Here, we investigated populations of mammary epithelial cells that are potential candidates for bovine mammary gland development using xenotransplantation into mice cleared mammary fat pad. Transplanted mammary explants from 17-month-old Holstein heifers developed outgrowths exhibiting the archetypal morphology and molecular marker distributions of the bovine gland. Xenotransplantation of sorted mammary epithelial cells (CD49fpos) into bovinised fat pads using inactivated bovine fibroblasts resulted in outgrowth developments with 50% take rate, but these lacked the ductal or alveolar epithelial structures of the normal mammary gland. Similar results were obtained with xenografts of candidate bovine mammary epithelial stem cells (CD49fhighCD24pos) or epithelial cells of the basal lineage (CD49fhighCD24neg) which also developed as clumps of cells surrounded by stromal stretches within the mouse adipose tissue. These results provide further evidence for the existence of cells with self-renewing capacities in the bovine mammary gland. Sorted cells, however, showed compromised regenerative potential for epithelial morphogenesis. Further work is therefore needed to identify mammary stem/progenitor cells with full regenerative capabilities for biogenesis of normal mammary gland structure, with milk-secreting function.
Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system
Authors: Davide Adamo , Giuseppe Genna Vincenzo , Giulia Galaverni , Chiara Chiavelli , Alessia Merra , Fabio Lepore , Federica Boraldi , Daniela Quaglino , Jessica Evangelista , Filippo Lococo , Graziella Pellegrini 
Published: December 2023

ABSTRACTThe clinical results of tissue engineering approaches aimed at treating extensive tracheal or bronchial defects highlighted some impactful limitations, among which stand out the inability to regenerate a functional and self-renewing airway epithelium onto the bioengineered graft. This outcome is often caused by culture conditions unable to sustain the long-term proliferative and differentiative potential of primary airway epithelial cells, resulting in progressive stem cell exhaustion. Here, we examined the capacity of a clinical-grade culture system to safely and efficiently support thein vitroexpansion of primary human tracheal and bronchial epithelial cells, regenerating a functional and fully differentiated airway epithelium. Moreover, we identified and characterized airway epithelial stem cells, unravelling the mechanisms that underlie tracheobronchial epithelium renewal. Finally, we suggested possible parameters to be employed as quality controls during critical stages of the tissue engineering process, thereby maximizing the likelihood of success of the treatment and prioritizing patients’ safety, crucial prerequisites before embarking on other clinical applications.GRAPHICAL ABSTRACT
Liquid-liquid Phase Separation of Alpha-synuclein Increases the Structural Variability of Fibrils Formed during Amyloid Aggregation
Authors: Ziaunys Mantas , Sulskis Darius , Veiveris Dominykas , Kopustas Aurimas , Snieckute Ruta , Mikalauskaite Kamile , Sakalauskas Andrius , Tutkus Marijonas , Smirnovas Vytautas 
Published: December 2023

AbstractProtein liquid-liquid phase separation (LLPS) is a rapidly emerging field of study on biomolecular condensate formation. In recent years, this phenomenon has been implicated in the process of amyloid fibril formation, serving as an intermediate step between the native protein transition into their aggregated state. The formation of fibrils via LLPS has been demonstrated for a number of proteins related to neurodegenerative disorders, as well as other amyloidoses. Despite the surge in amyloid-related LLPS studies, the influence of protein condensate formation on the end-point fibril characteristics is still far from fully understood. In this work, we compare alpha-synuclein aggregation under conditions, which promote or negate its LLPS and examine the differences between the formed aggregates. We show that alpha-synuclein phase separation generates a wide variety of assemblies with distinct secondary structures and morphologies. The LLPS-induced structures also possess higher levels of toxicity to cells, indicating that biomolecular condensate formation may be a critical step in the appearance of disease-related fibril variants.
Cultural variations in conceptualization of excessive drinking among young adults in Denmark, Estonia and Italy
Authors: Rolando Sara , Cuomo Gaia , Allaste Airi-Alina , Fabricius Venus Athena Vangsgaard , Kolind Torsten , Läänemets Merlin 
Published: December 2023

Purpose
This paper aims to investigate the cultural meanings of excessive drinking in three different countries with different levels of alcohol use chosen as case studies of wider geographies representing Northern (Denmark), Southern (Italy) and Eastern (Estonia) Europe.

Design/methodology/approach
Data were collected according to the Reception Analytical Group Interview method, using video clips as stimuli to enhance comparability. Eight online focus groups were organized in each country for a total number of 128 participants. Symbolic boundaries defining what drinking patterns are socially acceptable were then analysed to look at cross-national variations.

Findings
Results show how different conceptualizations of excessive drinking persist, although a convergence process among drinking patterns is also observed, which suggests that differences mainly depend on meanings and values attributed to intoxication. These are both rooted in the traditional drinking cultures and affected by ongoing social and economic change processes.

Research limitations/implications
Because of the chosen research approach, the research results may lack generalizability, even at country level, as there are differences also within the same drinking culture; however, addressing these differences was beyond the scope of the present study, which aimed to contribute to understanding persisting differences in European drinking culture despite different drivers seem to act for globalization of drinking habits.

Practical implications
The paper includes implications for the development of tailored and effective prevention messages, considering rooted attitudes and cultural values attached to drinking and drunkenness in different European geographies, which are also related to conceptualizations of risks and pleasure.

Originality/value
This paper fulfils an identified need to understand persisting differences in alcohol-related behaviours and outcome in different European countries emerging from quantitative data.
Employee psychological ownership and work attitudes
Authors: Rapti Andriana , Rayton Bruce A. , Yalabik Zeynep Yesim 
Published: December 2023

PurposeThis study aims to explain the link between employee psychological ownership (EPO) and work engagement (WE). The authors conceptualize EPO as a combination of various personal resources, i.e. self-efficacy, self-identity, accountability, belongingness and territoriality, and expected it to positively contribute to WE.Design/methodology/approachThe authors analyze survey data from employees of a public service organization in Greece and verify key findings using a two-wave cross-sectional survey design targeting a heterogeneous population of employees in the United States of America.FindingsThe findings indicate that EPO is positively related to WE and job satisfaction mediates the relationship between EPO and WE. Furthermore, the authors' results support the discriminant validity between promotive EPO, preventative EPO (territoriality), WE and job satisfaction.Originality/valueThe present study provides a deeper understanding of EPO, as it is explained in the theoretical foundations of the job demands-resources model (JD-R) model and helps us understand how EPO can affect WE.
How can facilities management benefit from offices becoming more user-centred?
Authors: Einola Kiia Aurora , Remes Laura , Dooley Kenneth 
Published: December 2023

Purpose
This study aims to explore an emerging collection of smart building technologies, known as smart workplace solutions (SWS), in the context of facilities management (FM).

Design/methodology/approach
This study is based on semi-structured interviews with facility managers in Finland, Norway and Sweden who have deployed SWSs in their organizations. SWS features, based on empirical data from a previous study, were also used to further analyse the interviews.

Findings
It analyses the benefits that SWSs bring from the facility management point of view. It is clear that the impetus for change and for deploying SWS in the context of FM is primarily driven by cost savings related to reductions in office space.

Research limitations/implications
This research has been conducted with a focus on office buildings only. However, other building types can learn from the benefits that facility managers receive in the area of user-centred smart buildings.

Practical implications
SWSs are often seen as employee experience solutions that are only related to “soft” elements such as collaboration, innovation and learning. Understanding the FM business case can help make a more practical case for their deployment.

Originality/value
SWSs are an emerging area, and this study has collected data from facility managers who use them daily.
Inside out. Social media videos and destination branding. Neuromarketing using EEG technique
Authors: Shahzad Muhammad Faisal , Yuan Jingbo , Arif Farrah , Waheed Abdul 
Published: December 2023

PurposeThis study aims to investigate the effectiveness of two types of social media videos used for destination image development: induced/commercial-oriented content and organic content (where content is made without commercial interest, such as vlogs classified as user-generated content).Design/methodology/approachExperimental research using “Emotive EEG” (electroencephalogram) in a controlled environment was conducted with 30 participants (20 males, 10 females), age range 18 to 26. Emotive EEG recording was performed while the participants watched both types of video clips. Test results for both groups indicate that induced content is preferred over organic content.FindingsThis study opens up future research avenues where neuromarketing’s “Marketer Friendly” EEG equipment can be applied to the customer selection process.Originality/valueMarketing analysts can gauge the interest and response of customers on different types of social media video content for destination marketing based on the findings of this study.
ANTi-microhistory of social innovation: humanistic education at Robert Owen’s New Harmony experiment
Authors: Roberts Foster B. , Novicevic Milorad M. , Humphreys John H. 
Published: December 2023

Purpose
The purpose of this study is to present ANTi-microhistory of social innovation in education within Robert Owen’s communal experiment at New Harmony, Indiana. The authors zoom out in the historical context of social innovation before zooming into the New Harmony case.

Design/methodology/approach
The authors used ANTi-microhistory approach to unpack the controversy around social innovation using the five-step procedure recently proposed by Mills et al. (2022), a version of the five-step procedure originally proposed by Tureta et al. (2021).

Findings
The authors found that the educational leaders of the New Harmony community preceded proponents of innovation, such as Drucker (1957) and Fairweather (1967), who viewed education as a form of social innovation.

Originality/value
The authors contribute to the history of social innovation in education by exploring the New Harmony community’s education society to uncover the enactment of sustainable social innovation and the origin story of humanistic management education.
Analysis and mapping of research on barriers to mental health service utilization in minority and underserved groups (1993-2022)
Authors: Sweileh Waleed 
Published: December 2023

Purpose
This paper aims to investigate research activity on barriers for minority and underserved groups to access and use mental health services.

Design/methodology/approach
Using Scopus, relevant articles published from 1993 to 2022 were collected. The final list included 122 articles.

Findings
Research hotspots included cultural and ethnic barriers, obstacles encountered by LGBTQ+ individuals, challenges faced by refugees and immigrants, limited access in rural areas and barriers affecting special populations. The top 10 cited articles focused on language barriers, cultural stigma, gender-specific challenges and systemic obstacles. New research avenues included the role of technology in overcoming barriers to access mental health services.

Practical implications
Policymakers and practitioners can use this knowledge to develop targeted interventions, enhance cultural competence, reduce stigma, improve rural access and provide LGBTQ+-affirming care, ultimately promoting equitable mental health care.

Social implications
This research underscores the importance of addressing mental health service barriers for equity and social justice. Neglecting these disparities can worsen mental health, increase health-care costs, reduce productivity and lead to higher social welfare expenses, perpetuating disadvantages.

Originality/value
This paper's uniqueness lies in its comprehensive analysis of barriers and facilitators to mental health service utilization among minority and underserved groups. It serves as a basis for developing evidence-based strategies to improve service accessibility and enhance the well-being of marginalized communities.
Letter: Are we forgetting the importance of steatosis in steatotic liver disease? Authors' reply
Authors: Kuo Selena Z. , Loomba Rohit 
Published: December 2023

LINKED CONTENTThis article is linked to Kuo et al papers. To view these articles, visit https://doi.org/10.1111/apt.17637 and https://doi.org/10.1111/apt.17759