bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of echinostomatid trematode eggs at the forest–oil palm interface in Sabah, Malaysia
Authors: Frias Liesbeth , Manin Benny Obrain , Guerrero-Sánchez Sergio , Sipangkui Symphorosa , Chua Tock H. 
Published: December 2023

Abstract
In this study, we report the occurrence of echinostomatid eggs in feces of wildlife, domestic animals and humans frequenting the forest–oil palm plantation interface in the Kinabatangan (Sabah, Malaysia), and discuss potential implications for public health. Using microscopy, we detected echinostomatid eggs in six host species, including Asian palm civets (Paradoxurus hermaphroditus [13/18]), leopard cats (Prionailurus bengalensis [3/4]), long-tailed macaques (Macaca fascicularis [1/10]), domestic dogs [3/5] and cats [1/1], and humans [7/9]. Molecular analysis revealed a close genetic proximity of civet echinostomatids to Artyfechinostomum malayanum, a zoonotic parasite of public health relevance. The intermediate hosts for A. malayanum have been reported in at least 3 districts in Sabah, suggesting that all the necessary elements required for the completion of the parasite's life cycle are present. Our findings point at the presence of zoonotic trematodes in an area with high human–wildlife interaction and highlight the potential public and animal health concern of zoonotic trematode infection in the context of Southeast Asia's rapidly changing ecosystems.
RODRIGUES FORMULA AND LINEAR INDEPENDENCE FOR VALUES OF HYPERGEOMETRIC FUNCTIONS WITH VARYING PARAMETERS
Authors: KAWASHIMA MAKOTO 
Published: December 2023

AbstractIn this article, we prove a generalized Rodrigues formula for a wide class of holonomic Laurent series, which yields a new linear independence criterion concerning their values at algebraic points. This generalization yields a new construction of Padé approximations including those for Gauss hypergeometric functions. In particular, we obtain a linear independence criterion over a number field concerning values of Gauss hypergeometric functions, allowing the parameters of Gauss hypergeometric functions to vary.
Choroidal and retinal thinning in chronic kidney disease independently associate with eGFR decline and are modifiable with treatment
Authors: Farrah Tariq E. , Pugh Dan , Chapman Fiona A. , Godden Emily , Balmforth Craig , Oniscu Gabriel C. , Webb David J. , Dhillon Baljean , Dear James W. , Bailey Matthew A. , Gallacher Peter J. , Dhaun Neeraj 
Published: December 2023

AbstractIn patients with chronic kidney disease (CKD), there is an unmet need for novel biomarkers that reliably track kidney injury, demonstrate treatment-response, and predict outcomes. Here, we investigate the potential of retinal optical coherence tomography (OCT) to achieve these ends in a series of prospective studies of patients with pre-dialysis CKD (including those with a kidney transplant), patients with kidney failure undergoing kidney transplantation, living kidney donors, and healthy volunteers. Compared to health, we observe similar retinal thinning and reduced macular volume in patients with CKD and in those with a kidney transplant. However, the choroidal thinning observed in CKD is not seen in patients with a kidney transplant whose choroids resemble those of healthy volunteers. In CKD, the degree of choroidal thinning relates to falling eGFR and extent of kidney scarring. Following kidney transplantation, choroidal thickness increases rapidly (~10%) and is maintained over 1-year, whereas gradual choroidal thinning is seen during the 12 months following kidney donation. In patients with CKD, retinal and choroidal thickness independently associate with eGFR decline over 2 years. These observations highlight the potential for retinal OCT to act as a non-invasive monitoring and prognostic biomarker of kidney injury.
Chiral, air stable, and reliable Pd(0) precatalysts applicable to asymmetric allylic alkylation chemistry
Authors: Huang Jingjun , Keenan Thomas , Richard François , Lu Jingru , Jenny Sarah E. , Jean Alexandre , Arseniyadis Stellios , Leitch David C. 
Published: December 2023

AbstractStereoselective carbon-carbon bond formation via palladium-catalyzed asymmetric allylic alkylation is a crucial strategy to access chiral natural products and active pharmaceutical ingredients. However, catalysts based on the privileged Trost and Pfaltz-Helmchen-Williams PHOX ligands often require high loadings, specific preactivation protocols, and excess chiral ligand. This makes these reactions uneconomical, often unreproducible, and thus unsustainable. Here we report several chiral single-component Pd(0) precatalysts that are active and practically-applicable in a variety of asymmetric allylic alkylation reactions. Despite the decades-long history and widespread use of Trost-type ligands, the precatalysts in this work are the only reported examples of stable, isolable Pd(0) complexes with these ligands. Evaluating these precatalysts across nine asymmetric allylic alkylation reactions reveals high reactivity and selectivity at low Pd loading. Importantly, we also report an unprecedented Pd-catalyzed enantioselective allylation of a hydantoin, achieved on gram scale in high yield and enantioselectivity with only 0.2 mol% catalyst.
Incommensurate grain-boundary atomic structure
Authors: Seki Takehito , Futazuka Toshihiro , Morishige Nobusato , Matsubara Ryo , Ikuhara Yuichi , Shibata Naoya 
Published: December 2023

AbstractGrain-boundary atomic structures of crystalline materials have long been believed to be commensurate with the crystal periodicity of the adjacent crystals. In the present study, we experimentally observed a Σ9 grain-boundary atomic structure of a bcc crystal (Fe-3%Si). It is found that the Σ9 grain-boundary structure is largely reconstructed and forms a dense packing of icosahedral clusters in its core. Combining with the detailed theoretical calculations, the Σ9 grain-boundary atomic structure is discovered to be incommensurate with the adjacent crystal structures. The present findings shed new light on the study of stable grain-boundary atomic structures in crystalline materials.
Early-adult methionine restriction reduces methionine sulfoxide and extends lifespan in Drosophila
Authors: Kosakamoto Hina , Obata Fumiaki , Kuraishi Junpei , Aikawa Hide , Okada Rina , Johnstone Joshua N. , Onuma Taro , Piper Matthew D. W. , Miura Masayuki 
Published: December 2023

AbstractMethionine restriction (MetR) extends lifespan in various organisms, but its mechanistic understanding remains incomplete. Whether MetR during a specific period of adulthood increases lifespan is not known. In Drosophila, MetR is reported to extend lifespan only when amino acid levels are low. Here, by using an exome-matched holidic medium, we show that decreasing Met levels to 10% extends Drosophila lifespan with or without decreasing total amino acid levels. MetR during the first four weeks of adult life only robustly extends lifespan. MetR in young flies induces the expression of many longevity-related genes, including Methionine sulfoxide reductase A (MsrA), which reduces oxidatively-damaged Met. MsrA induction is foxo-dependent and persists for two weeks after cessation of the MetR diet. Loss of MsrA attenuates lifespan extension by early-adulthood MetR. Our study highlights the age-dependency of the organismal response to specific nutrients and suggests that nutrient restriction during a particular period of life is sufficient for healthspan extension.
Copper assisted sequence-specific chemical protein conjugation at a single backbone amide
Authors: Guo Mengzhun , Zhao Kai , Guo Liang , Zhou Rui , He Qiuju , Lu Kuan , Li Tian , Liu Dandan , Chen Jinfeng , Tang Jing , Fu Xin , Zhou Jinyun , Zheng Bei , Mann Samuel I. , Zhang Yongdeng , Huang Jing , Yang Bing , Zhou Ting , Lei Yingjie , Dang Bobo 
Published: December 2023

AbstractDirect, site-specific methods of protein functionalization are highly desirable for biotechnology. However, such methods are challenging due to the difficulty of chemically differentiating a single site within a large protein. Herein, we propose “metal binding targeting” strategy and develop a Copper Assisted Sequence-specific conjugation Tag (CAST) method to achieve rapid (second order rate 8.1 M−1 s−1), site-specific protein backbone chemical modification with pinpoint accuracy. We demonstrate the versatility of CAST conjugation by preparing various on-demand modified recombinant proteins, including a homogeneous antibody-drug conjugate with high plasma stability and potent efficacy in vitro and in vivo. Thus, CAST provides an efficient and quantitative method to site-specifically attach payloads on large, native proteins.
Ultraflexible, cost-effective and scalable polymer-based phase change composites via chemical cross-linking for wearable thermal management
Authors: Jing Yaoge , Zhao Zhengchuang , Cao Xiaoling , Sun Qinrong , Yuan Yanping , Li Tingxian 
Published: December 2023

AbstractPhase change materials (PCMs) offer great potential for realizing zero-energy thermal management due to superior thermal storage and stable phase-change temperatures. However, liquid leakage and solid rigidity of PCMs are long-standing challenges for PCM-based wearable thermal regulation. Here, we report a facile and cost-effective chemical cross-linking strategy to develop ultraflexible polymer-based phase change composites with a dual 3D crosslinked network of olefin block copolymers (OBC) and styrene-ethylene-butylene-styrene (SEBS) in paraffin wax (PW). The C-C bond-enhanced OBC-SEBS networks synergistically improve the mechanical, thermal, and leakage-proof properties of PW@OBC-SEBS. Notably, the proposed peroxide-initiated chemical cross-linking method overcomes the limitations of conventional physical blending methods and thus can be applicable across diverse polymer matrices. We further demonstrate a portable and flexible PW@OBC-SEBS module that maintains a comfortable temperature range of 39–42 °C for personal thermotherapy. Our work provides a promising route to fabricate scalable polymer-based phase change composite for wearable thermal management.
Functional divergence of a bacterial enzyme promotes healthy or acneic skin
Authors: Hajam Irshad A. , Katiki Madhusudhanarao , McNally Randall , Lázaro-Díez María , Kolar Stacey , Chatterjee Avradip , Gonzalez Cesia , Paulchakrabarti Mousumi , Choudhury Biswa , Caldera J. R. , Desmond Trieu , Tsai Chih-Ming , Du Xin , Li Huiying , Murali Ramachandran , Liu George Y. 
Published: December 2023

AbstractAcne is a dermatologic disease with a strong pathologic association with human commensal Cutibacterium acnes. Conspicuously, certain C. acnes phylotypes are associated with acne, whereas others are associated with healthy skin. Here we investigate if the evolution of a C. acnes enzyme contributes to health or acne. Two hyaluronidase variants exclusively expressed by C. acnes strains, HylA and HylB, demonstrate remarkable clinical correlation with acne or health. We show that HylA is strongly pro-inflammatory, and HylB is modestly anti-inflammatory in a murine (female) acne model. Structural and phylogenic studies suggest that the enzymes evolved from a common hyaluronidase that acquired distinct enzymatic activity. Health-associated HylB degrades hyaluronic acid (HA) exclusively to HA disaccharides leading to reduced inflammation, whereas HylA generates large-sized HA fragments that drive robust TLR2-dependent pathology. Replacing an amino acid, Serine to Glycine near the HylA catalytic site enhances the enzymatic activity of HylA and produces an HA degradation pattern intermediate to HylA and HylB. Selective targeting of HylA using peptide vaccine or inhibitors alleviates acne pathology. We suggest that the functional divergence of HylA and HylB is a major driving force behind C. acnes health- and acne- phenotype and propose targeting of HylA as an approach for acne therapy.
Phox2b-expressing neurons contribute to breathing problems in Kcnq2 loss- and gain-of-function encephalopathy models
Authors: Soto-Perez J. , Cleary C. M. , Sobrinho C. R. , Mulkey S. B. , Carroll J. L. , Tzingounis A. V. , Mulkey D. K. 
Published: December 2023

AbstractLoss- and gain-of-function variants in the gene encoding KCNQ2 channels are a common cause of developmental and epileptic encephalopathy, a condition characterized by seizures, developmental delays, breathing problems, and early mortality. To understand how KCNQ2 dysfunction impacts behavior in a mouse model, we focus on the control of breathing by neurons expressing the transcription factor Phox2b which includes respiratory neurons in the ventral parafacial region. We find Phox2b-expressing ventral parafacial neurons express Kcnq2 in the absence of other Kcnq isoforms, thus clarifying why disruption of Kcnq2 but not other channel isoforms results in breathing problems. We also find that Kcnq2 deletion or expression of a recurrent gain-of-function variant R201C in Phox2b-expressing neurons increases baseline breathing or decreases the central chemoreflex, respectively, in mice during the light/inactive state. These results uncover mechanisms underlying breathing abnormalities in KCNQ2 encephalopathy and highlight an unappreciated vulnerability of Phox2b-expressing ventral parafacial neurons to KCNQ2 pathogenic variants.
Secretory GFP reconstitution labeling of neighboring cells interrogates cell–cell interactions in metastatic niches
Authors: Minegishi Misa , Kuchimaru Takahiro , Nishikawa Kaori , Isagawa Takayuki , Iwano Satoshi , Iida Kei , Hara Hiromasa , Miura Shizuka , Sato Marika , Watanabe Shigeaki , Shiomi Akifumi , Mabuchi Yo , Hamana Hiroshi , Kishi Hiroyuki , Sato Tatsuyuki , Sawaki Daigo , Sato Shigeru , Hanazono Yutaka , Suzuki Atsushi , Kohro Takahide , Kadonosono Tetsuya , Shimogori Tomomi , Miyawaki Atsushi , Takeda Norihiko , Shintaku Hirofumi , Kizaka-Kondoh Shinae , Nishimura Satoshi 
Published: December 2023

AbstractCancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in the metastatic niche are yet to be fully elucidated owing to the lack of methodologies for comprehensively studying the mechanisms of cell–cell interactions in the niche. Here, we improve a split green fluorescent protein (GFP)-based genetically encoded system to develop secretory glycosylphosphatidylinositol-anchored reconstitution-activated proteins to highlight intercellular connections (sGRAPHIC) for efficient fluorescent labeling of tissue-resident cells that neighbor on and putatively interact with cancer cells in deep tissues. The sGRAPHIC system enables the isolation of metastatic niche-associated tissue-resident cells for their characterization using a single-cell RNA sequencing platform. We use this sGRAPHIC-leveraged transcriptomic platform to uncover gene expression patterns in metastatic niche-associated hepatocytes in a murine model of liver metastasis. Among the marker genes of metastatic niche-associated hepatocytes, we identify Lgals3, encoding galectin-3, as a potential pro-metastatic factor that accelerates metastatic growth and invasion.
Electrochemical nitrate reduction in acid enables high-efficiency ammonia synthesis and high-voltage pollutes-based fuel cells
Authors: Zhang Rong , Li Chuan , Cui Huilin , Wang Yanbo , Zhang Shaoce , Li Pei , Hou Yue , Guo Ying , Liang Guojin , Huang Zhaodong , Peng Chao , Zhi Chunyi 
Published: December 2023

AbstractMost current research is devoted to electrochemical nitrate reduction reaction for ammonia synthesis under alkaline/neutral media while the investigation of nitrate reduction under acidic conditions is rarely reported. In this work, we demonstrate the potential of TiO2 nanosheet with intrinsically poor hydrogen-evolution activity for selective and rapid nitrate reduction to ammonia under acidic conditions. Hybridized with iron phthalocyanine, the resulting catalyst displays remarkably improved efficiency toward ammonia formation owing to the enhanced nitrate adsorption, suppressed hydrogen evolution and lowered energy barrier for the rate-determining step. Then, an alkaline-acid hybrid Zn-nitrate battery was developed with high open-circuit voltage of 1.99 V and power density of 91.4 mW cm–2. Further, the environmental sulfur recovery can be powered by above hybrid battery and the hydrazine-nitrate fuel cell can be developed for simultaneously hydrazine/nitrate conversion and electricity generation. This work demonstrates the attractive potential of acidic nitrate reduction for ammonia electrosynthesis and broadens the field of energy conversion.
NONAN GaitPrint: An IMU gait database of healthy young adults
Authors: Wiles Tyler M. , Mangalam Madhur , Sommerfeld Joel H. , Kim Seung Kyeom , Brink Kolby J. , Charles Anaelle Emeline , Grunkemeyer Alli , Kalaitzi Manifrenti Marilena , Mastorakis Spyridon , Stergiou Nick , Likens Aaron D. 
Published: December 2023

AbstractAn ongoing thrust of research focused on human gait pertains to identifying individuals based on gait patterns. However, no existing gait database supports modeling efforts to assess gait patterns unique to individuals. Hence, we introduce the Nonlinear Analysis Core (NONAN) GaitPrint database containing whole body kinematics and foot placement during self-paced overground walking on a 200-meter looping indoor track. Noraxon Ultium MotionTM inertial measurement unit (IMU) sensors sampled the motion of 35 healthy young adults (19–35 years old; 18 men and 17 women; mean ± 1 s.d. age: 24.6 ± 2.7 years; height: 1.73 ± 0.78 m; body mass: 72.44 ± 15.04 kg) over 18 4-min trials across two days. Continuous variables include acceleration, velocity, position, and the acceleration, velocity, position, orientation, and rotational velocity of each corresponding body segment, and the angle of each respective joint. The discrete variables include an exhaustive set of gait parameters derived from the spatiotemporal dynamics of foot placement. We technically validate our data using continuous relative phase, Lyapunov exponent, and Hurst exponent—nonlinear metrics quantifying different aspects of healthy human gait.
Emissions of pesticides in the European Union: a new regional-level dataset
Authors: Udias Angel , Galimberti Francesco , Dorati Chiara , Pistocchi Alberto 
Published: December 2023

AbstractWe present a European Union (EU)-wide dataset of estimated quantities of active substances of plant protection product applied on crops (also called “emissions”). Our estimates are derived from data reported by eight EU countries and extrapolated to encompass all EU regions using regression models. These models consider both climate and agricultural land use data. This allows us to spatially represent pesticide use at NUTS Level 3 of the European statistical mapping units, and within various agricultural land cover classes in each region. We compare our estimates with aggregated data provided by EUROSTAT and with independent, detailed data for the United Kingdom, highlighting an error typically within one order of magnitude. Our estimates can provide insights into the distribution and patterns of pesticide use in the EU around the year 2015. The estimate is most reliable for Western and Southern Europe. Outside these regions, data scarcity makes extrapolation more uncertain, potentially limiting the ability to accurate depict regional variations in pesticide use.
Heterogeneous-elasticity theory of instantaneous normal modes in liquids
Authors: Mossa Stefano , Bryk Taras , Ruocco Giancarlo , Schirmacher Walter 
Published: December 2023

AbstractSince decades, the concept of vibrational density of states in glasses has been mirrored in liquids by the instantaneous-normal-mode spectrum. In glasses instantaneous configurations are believed to be situated close to minima of the potential-energy hypersurface and all eigenvalues of the associated Hessian matrix are positive. In liquids this is no longer true, and modes corresponding to both positive and negative eigenvalues exist. The instantaneous-normal-mode spectrum has been numerically investigated in the past, and it has been demonstrated to bring important information on the liquid dynamics and transport properties. A systematic deeper theoretical understanding is now needed. Heterogeneous-elasticity theory has proven to be particularly successful in explaining many details of the low-frequency excitations in glasses, ranging from the thoroughly studied boson peak, to other anomalies related to the crossover between wave-like and random-matrix-like excitations. Here we present an extension of heterogeneous-elasticity theory to the liquid state, and show that the outcome of the theory agrees well to the results of extensive molecular-dynamics simulations of a model liquid at different temperatures. We find that the spectrum of eigenvalues $$\rho (\lambda )$$

ρ
(
λ
)

has a sharp maximum close to (but not at) $$\lambda =0$$

λ
=
0

, and decreases monotonically with $$|\lambda |$$

|
λ
|

on both its stable and unstable side. We show that the spectral shape strongly depends on temperature, being symmetric at high temperatures and becoming rather asymmetric at low temperatures, close to the dynamical critical temperature. Most importantly, we demonstrate that the theory naturally reproduces a surprising phenomenon, a zero-energy spectral singularity with a cusp-like character developing in the vibrational spectra upon cooling. This feature, known from a few previous numerical studies, has been generally overlooked in the past due to a misleading representation of the data. We provide a thorough analysis of this issue, based on both very accurate predictions of our theory, and computational studies of model liquid systems with extended size.
Study on re-entrant hierarchical honeycombs in-plane impact
Authors: Lian Jinming , Xu Lei , Wu Donggao , Wang Zhenqing 
Published: December 2023

AbstractThe introduction of hierarchical structure in cell materials can further improve their energy absorption effect, and negative Poisson's ratio materials have excellent energy absorption capacity and special deformation mode. In this paper, augmented double arrow honeycomb structures is introduced into the re-entrant honeycomb with negative Poisson's ratio as a substructure (RHA) to improve the mechanical properties of the first-order re-entrant honeycomb and enhance the energy absorption effect of the structure. The analytical formula of the collapse stress of honeycomb under quasi-static compression was derived by the two-scale method. The failure stress of RHA under different relative densities and impact velocities is discussed, and the analytical formula of RHA stress in dynamic crushing is derived by combining momentum conservation. Due to the special substructure, the secondary honeycomb discussed in this paper has two plateau periods. In this paper, the second plateau stress of the honeycomb structure is calculated innovatively. The numerical simulation results show that the collapse stress of RHA in the first plateau period is similar to that of the first-order re-entrant honeycomb, and the collapse stress in the second plateau period is increased by 332%. The research in this paper shows that the honeycomb with the second plateau period has a better energy absorption effect, which is an effective strategy for improving the energy absorption effect of the honeycomb. It can be further explored to improve the impact resistance of the honeycomb.