bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chalcones as potent antiplatelet agents and calcium channel blockers
Authors: Lin Chun‐Nan , Hsieh Hsin‐Kaw , Ko Horng‐Huey , Hsu Mei‐Feng , Lin Hsien‐Cheng , Chang Ya‐Ling , Chung Mei‐Ing , Kang Jaw‐Jou , Wang Jih‐Pyang , Teng Che‐Ming 
Published: September 2002

AbstractIn an effort to continually develop potent antiplatelet agents with vasorelaxing and antiinflammatory actions, a novel series of antiinflammatory chalcones was continually screened to evaluate their antiplatelet and vasorelaxing effects. Their structure–activity relationships and mode of action were discussed and characterized. A novel series of antiinflammatory chalcones was studied on antiplatelet effect in rabbit washed platelets and human platelet‐rich plasma (PRP) and vasorelaxing effect in rat thoracic aorta. Arachidonic acid‐induced platelet aggregation was potently inhibited by almost all the chalcone derivatives and 13–15 also had a potent inhibitory effect on cyclooxygenase. The selective chalcones 12–16 tested in human PRP significantly inhibited secondary aggregation induced by adrenaline. In rat thoracic aorta, most of chalcones at high concentration significantly depressed the contractions induced by Ca2+ (1.9 mM) in high K+ (80 mM) medium and the phasic and tonic contractions caused by norepinephrine (3 μM). In the rat thoracic aorta, the phenylephrine‐ and high K+‐induced 45Ca2+ influx were both inhibited by a selective chalcone derivative, 14. These results indicate that the antiplatelet actions of chalcones are mainly mediated through the suppression of cyclooxygenase activity and reduced thromboxane formation and their inhibitory effects on the contractile response caused by high K+ and norepinephrine in rat thoracic aorta are mainly due to inhibition of Ca2+ influx through both voltage‐dependent and receptor‐operated Ca2+ channels. Drug Dev. Res. 53:9–14, 2001. © 2001 Wiley‐Liss, Inc.
Characterization and expression of ATP P2X4 receptor from embryonic chick skeletal muscle
Authors: Bo Xuenong , Liu Min , Schoepfer Ralf , Burnstock Geoffrey 
Published: September 2002

AbstractPrevious pharmacological experiments have indicated the existence of ATP P2X receptors in chick embryonic skeletal muscles. In this study we cloned a P2X4‐like cDNA encoding a protein of 385 amino acids, which shares 75% and 76% identity with rat and human P2X4 receptors, respectively. Functional studies of this cP2X4 receptor expressed in Xenopus oocytes showed that ATP induced a fast inward current, which was partially desensitized upon prolonged application of ATP. The ATP‐induced currents were concentration‐dependent, with an EC50 of 9.5 μM. Adenosine 5′‐O‐(thio)triphosphate and 2‐methylthioATP very weak agonists. α,β‐methyleneATP was almost inactive. In contrast to their potentiating effects on recombinant rat P2X4 receptors, both suramin and pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulfonic acid partially blocked ATP‐induced currents. TrinitrophenylATP was able to block ATP‐induced response completely, with an IC50 of 4.7 μM. Northern blot and RT‐PCR analysis showed that cP2X4 mRNAs were mainly expressed in skeletal muscle, brain, and gizzard of day 10 chick embryos. Lower levels of expression were also detected in liver, heart, and retina. Whole‐mount in situ hybridization showed that cP2X4 mRNAs were expressed in the brain, spinal cord, notochord, gizzard, and skeletal muscle. The physiological functions of cP2X4 receptors in embryonic skeletal muscle remain unclear at present. Drug Dev. Res. 53:22–28, 2001. © 2001 Wiley‐Liss, Inc.
Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation
Authors: Della Gaspera Bruno , Braut‐Boucher Françoise , Bomsel Morgane , Chatelet Françoise , Guguen‐Guillouzo Christiane , Font Jacqueline , Weinman Jacqueline , Weinman Serge 
Published: September 2002

AbstractAnnexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached low levels thereafter. However, Anx 5 and 6 rates were more constant. Prenatal administration of dexamethasone (dex) resulted in a decrease of Anx 1 mRNA levels, and a strong increase in Anx 2 mRNA contents. In adult hepatocytes cultured in the presence of EGF or HGF, Anx 1 and 2 expressions resumed. By immunohistochemistry, Anx 1 was detected only in the cytoplasm of hepatocytes of 1‐ to 3‐day‐old rats, Anx 2 and 6 both exhibited a redistribution from the cytoplasm toward the plasma membrane, and Anx 5 was present in the nucleus, cytoplasm, and plasma membrane. Thus, Anx 1, 2, 5, and 6 have individual modes of expression and localization in the differentiating hepatocytes, where they might play unique roles at well defined phases of liver ontogeny. © 2001 Wiley‐Liss, Inc.
Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF or ICSI
Authors: Suemori Hirofumi , Tada Takashi , Torii Ryuzo , Hosoi Yoshihiko , Kobayashi Kinji , Imahie Hiroshi , Kondo Yasushi , Iritani Akira , Nakatsuji Norio 
Published: September 2002

AbstractHuman embryonic stem (ES) cells are predicted to be a valuable source for producing ES‐derived therapeutic spare tissues to treat diseases by controlling their growth and differentiation. To understand the regulative mechanisms of their differentiation in vivo and in vitro, ES cells derived from nonhuman primates could be a powerful tool. We established four ES cell lines from cynomolgus monkey (Macaca fascicularis) blastocysts produced by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The ES cells were characterized by the expression of specific markers such as alkaline phosphatase and stage‐specific embryonic antigen‐4. They were successfully maintained in an undifferentiated state and with a normal karyotype even after more than 6 months of culture. Pluripotential competence was confirmed by the formation of teratomas containing ectoderm‐, mesoderm‐, and endoderm‐ derivatives after subcutaneous injection into SCID mice. Differentiation to a variety of tissues was identified by immunohistochemical analyses using tissue‐specific antibodies. Therefore, we established pluripotent ES cell lines derived from monkeys that are widely used as experimental animals. These lines could be a useful resource for preclinical stem cell research, including allogenic transplantation into monkey models of disease. © 2001 Wiley‐Liss, Inc.
NG2 proteoglycan is expressed exclusively by mural cells during vascular morphogenesis
Authors: Ozerdem Ugur , Grako Kathryn A. , Dahlin‐Huppe Kimberlee , Monosov Edward , Stallcup William B. 
Published: September 2002

AbstractImmunofluorescence mapping demonstrates that the NG2 proteoglycan is invariably expressed by the mural cell component of mouse neovascular structures. This pattern is independent of the developmental mechanism responsible for formation of the vasculature (vasculogenesis or angiogenesis). Thus, NG2 is expressed in the embryonic heart by cardiomyocytes, in developing macrovasculature by smooth muscle cells, and in nascent microvessels by vascular pericytes. Due to the scarcity of proven markers for developing pericytes, NG2 is especially useful for identification of this cell type. The utility of NG2 as a pericyte marker is illustrated by two observations. First, pericytes are associated with endothelial tubes at an early point in microvessel development. This early interaction between pericytes and endothelial cells has important implications for the role of pericytes in the development and stabilization of microvascular tubes. Second, the pericyte to endothelial cell ratio in developing capillaries varies from tissue to tissue. Because the extent of pericyte investment is likely to affect the physical properties of the vessel in question, it is important to understand the mechanisms that control this process. Additional insight into these and other aspects of vascular morphogenesis should be possible through use of NG2 as a mural cell marker. © 2001 Wiley‐Liss, Inc.
Induction of bud formation of embryonic mouse tracheal epithelium by fibroblast growth factor plus transferrin in mesenchyme‐free culture
Authors: Ohtsuka Norie , Urase Koko , Momoi Takashi , Nogawa Hiroyuki 
Published: September 2002

AbstractEmbryonic mouse tracheal epithelium, which branches in an epithelial–mesenchymal recombination culture with bronchial mesenchyme, was cultured under mesenchyme‐free conditions. When embedded in a basement‐membrane–like matrix and cultured in a serum‐free medium supplemented with fibroblast growth factor 1 (FGF1), the tracheal epithelium did not branch, whereas the bronchial epithelium underwent branching morphogenesis. When the medium was enriched with transferrin (Tf), bud formation was induced in the tracheal epithelium and some buds branched secondarily. FGF7 and FGF10, in cooperation with Tf, induced tracheal bud formation to the same extent as FGF1, although the optimum concentrations differed. A bromodeoxyuridine‐labeling study comparing cultures with and without Tf showed no Tf‐specific amplification of cell proliferation. A whole‐mount in situ hybridization study of the expression of Bmp4 and Shh genes in explants of mesenchyme‐free culture revealed that both genes were ubiquitously expressed and that expression did not correlate with bud formation. This expression pattern was different from the distally localized expression pattern observed in normal lung rudiments and in extratracheal buds induced by the recombined bronchial mesenchyme. These results suggest that both bronchial and tracheal bud formations were initiated without localized exposure of the epithelium to FGFs and were not accompanied by localized expression of Bmp4 and Shh in the epithelium. © 2001 Wiley‐Liss, Inc.
Body image and personality predictors of eating disorder symptoms during the college years
Authors: Cooley Eric , Toray Tamina 
Published: September 2002

AbstractObjectiveWomen entering college (N = 118) were longitudinally followed for 3 years with assessments of eating pathology (Restraint and Bulimia).MethodMeasures taken at Time 1 included timing of onset of puberty, Figure Dissatisfaction, Ineffectiveness, Public Self‐Consciousness, and mood states (Profile of Mood States). Measures were evaluated as predictors of changes in Restraint and Bulimia scores across the three‐year period.ResultsBoth Restraint (r = .69) and Bulimia (r = .50) were quite stable across the 3 years. Mean weight gain of 5.4 pounds (p <.001) was paralleled by an increase in “ideal weight” of 4.7 pounds. Hierarchical regressions controlling for Time 1 levels of eating disorder symptoms revealed that changes in Bulimia (R2 = .38, p <.0001) were related to the psychological measures and to Figure Dissatisfaction. Changes in Restraint (R2 = .61, p <.0001) were significantly related to Figure Dissatisfaction.DiscussionFindings are discussed in the context of the value of longitudinal designs in identifying risk factors. Dissatisfaction with one's figure seems to be consistently related to worsening eating pathology. © 2001 by John Wiley & Sons, Inc. Int J Eat Disord 30: 28–36, 2001.
Altered response to meta‐chlorophenylpiperazine in anorexia nervosa: Support for a persistent alteration of serotonin activity after short‐term weight restoration
Authors: Frank Guido K. , Kaye Walter H. , Weltzin Theodore E. , Perel James , Moss Howard , McConaha Claire , Pollice Christine 
Published: September 2002

AbstractObjectivePatients with anorexia nervosa (AN) have disturbances of appetite and behaviors, such as dysphoria, inhibition, and obsessions, that could be related to altered serotonin activity. To investigate such relationships, we administered meta‐chlorophenylpiperazine (m‐CPP), a relatively serotonin‐specific drug.MethodsTo avoid the confounding effects of malnutrition or weight loss, we studied 12 patients with restricting‐type AN between 5 and 17 days after a return to a normal weight and while on a stable dietary intake. We compared them to 12 healthy control women (CW). m‐CPP was administered double blind and placebo controlled.ResultsAlthough weight restored, AN women had lower body weight and increased ratings for depression and obsessionality compared with CW. After m‐CPP, AN women had an elevation in mood and a reduction in body image distortion when compared with placebo. After m‐CPP, groups had similar cortisol, adrenocorticotropin (ACTH), and growth hormone responses whereas AN women had an uncertain reduction in prolactin response.DiscussionThese data support other studies that suggest that altered serotonin activity persists after weight restoration in AN patients. The finding that m‐CPP temporarily improved mood and reduced body image distortions supports the hypothesis that altered serotonin activity may contribute to the pathophysiology of AN. © 2001 by John Wiley & Sons, Inc. Int J Eat Disord 30: 57–68, 2001.
Effects of a circuit weight training program on the body images of college students
Authors: Williams Pamela A. , Cash Thomas F. 
Published: September 2002

AbstractObjectiveThe present investigation examined the extent to which participation in a 6‐week circuit‐weight training program produced changes in participants' body images relative to a matched control group.MethodThe weight trainers consisted of 39 college students (27 women and 12 men). The control group of 39 individuals did not weight‐train currently or within the past year. All participants were pre‐ and posttested on the Multidimensional Body Self‐Relations Questionnaire, the Social Physique Anxiety Scale, and the Physical Self‐Efficacy Scale. Weight trainers were also pre‐ and posttested on muscular strength and assessed on their motives for exercise.ResultsThe program successfully increased upper‐ and lower‐body strength. In contrast to the comparison group, weight trainers had a significantly improved evaluation of their appearance, greater body satisfaction, reduced social physique anxiety, and enhanced physical self‐efficacy. Outcomes were unrelated to the extent of concurrent aerobic exercise and largely unrelated to exercise motives.DiscussionEven a relatively brief weight training program can produce improvements in multiple aspects of body image. Further research should investigate weight training as an adjunct to psychosocial treatments of body dissatisfaction. © 2001 by John Wiley & Sons, Inc. Int J Eat Disord 30: 75–82, 2001.
Dialectical behavior therapy adapted for bulimia: A case report
Authors: Safer Debra L. , Telch Christy F. , Agras W. Stewart 
Published: September 2002

AbstractObjectiveThis case report describes the application of dialectical behavior therapy (DBT) to the treatment of bulimia nervosa in a 20‐session manualized therapy.MethodThe treatment, based on an affect regulation model of eating disorders, was developed to teach emotion regulation skills to replace eating‐disordered behaviors. The patient, a 36‐year‐old woman, had a long history of binge eating and purging that had not responded to 2 years of counseling. In the 4 weeks before treatment began, she reported 13 objective binges and 21 purging episodes.Results and DiscussionUpon initiating DBT, her binge eating and purging rapidly declined. She achieved abstinence by the fifth week of treatment and maintained it through treatment. In the 6 months following treatment, she reported a total of two objective binge episodes and two purge episodes. © 2001 by John Wiley & Sons, Inc. Int J Eat Disorder 30: 101–106, 2001.
Aggravation of food‐related behavior in an adolescent with Prader‐Willi syndrome treated with fluvoxamine and fluoxetine
Authors: Kohn Yoav , Weizman Abraham , Apter Alan 
Published: September 2002

AbstractPrader‐Willi Syndrome is a congenital multisystem disorder, characterized by a typical dysmorphism, mental retardation, hyperphagia due to insatiable appetite, and resultant morbid obesity. Psychiatric symptoms include obsessions and temper tantrums.MethodPharmacotherapy is experimental with a few reports of successful fluoxetine treatment.ResultsWe report an aggravation in the food‐related symptoms and a consequent weight gain in an adolescent with Prader‐Willi syndrome, who was treated with fluvoxamine and fluoxetine. © 2001 by John Wiley & Sons, Inc. Int J Eat Disord 30: 113–117, 2001.
Adaptive view‐dependent display of polygon models
Authors: Yoshida Norimasa , Kitajima Katsuhiro 
Published: September 2002

AbstractIn this paper, we introduce an adaptive view‐dependent display algorithm for polygon models. Although polygon models are being ubiquitously used in various kinds of applications, they are becoming increasingly complex. To display such polygon models at an interactive rate, not only improvements of three‐dimensional graphics accelerators but also various kinds of software techniques are inevitable. By applying a fast object‐precision simplification algorithm to our method, we present an adaptive view‐dependent display method of polygon models that requires a small amount of preprocessing time. We show how to adaptively simplify or refine polygon models in real time and results applied to several models. © 2001 Scripta Technica, Electron Comm Jpn Pt 2, 84(8): 1–9, 2001
A proposal for extracting an object's surface from stereo degree‐of‐correspondence space considering occlusions
Authors: Katayama Yasuhiro , Okutomi Masatoshi 
Published: September 2002

AbstractThe ambiguity of correspondence assignment is alleviated by utilizing the state of the neighboring correspondence of the focal point (local support) in detecting the points of correspondence in stereo vision. However, in detecting the area base points of correspondence with evaluations of the correspondence using windows, specific local supports are utilized, which can interfere with the correct correspondence assignments. In this paper, a scheme for performing correspondence assignments considering local supports which coincide correctly with the surface of an object is proposed. In this scheme, the surface of the object is directly detected as a surface model, using a new proposed GA detection scheme from the stereo degree‐of‐correspondence space in which the degree of correspondence is expressed as three‐dimensional data. As a result, correspondence assignment is performed with correct local supports by evaluating various surface models obtained by GA search from the stereo degree‐of‐correspondence space. In addition, the areas in which occlusions have occurred are specified by considering uniformity requirements in searching for surface models. Thus, a method for improving the areas containing occlusions utilizing multiple‐camera stereo vision is proposed. In this paper, the construction of the stereo degree‐of‐correspondence space, the GA search scheme, and the specification and improvement of occlusions are presented and the results of simulation experiments with objects of simple shapes are presented. © 2001 Scripta Technica, Electron Comm Jpn Pt 2, 84(8): 38–48, 2001
Achievement orientations from subjective histories of success: Promotion pride versus prevention pride
Authors: Higgins E. Tory , Friedman Ronald S. , Harlow Robert E. , Idson Lorraine Chen , Ayduk Ozlem N. , Taylor Amy 
Published: September 2002

AbstractA new task goal elicits a feeling of pride in individuals with a subjective history of success, and this achievment pride produces anticipatory goal reactions that energize and direct behavior to approach the task goal. By distinguishing between promotion pride and prevention pride, the present paper extends this classic model of achievement motivation. Regulatory focus theory (Higgins, 1997) distinguishes between a promotion focus on hopes and accomplishments (gains) and a prevention focus on safety and responsibilities (non‐losses). We propose that a subjective history of success with promotion‐related eagerness (promotion pride) orients individuals toward using eagerness means to approach a new task goal, whereas a subjective history of success with prevention‐related vigilance (prevention pride) orients individuals toward using vigilance means to approach a new task goal. Studies 1–3 tested this proposal by examining the relations between a new measure of participants' subjective histories of promotion success and prevention success (the Regulatory Focus Questionnaire (RFQ)) and their achievement strategies in different tasks. Study 4 examined the relation between participants' RFQ responses and their reported frequency of feeling eager or vigilant in past task engagements. Study 5 used an experimental priming technique to make participants temporarily experience either a subjective history of promotion success or a subjective history of prevention success. For both chronic and situationally induced achievement pride, these studies found that when approaching task goals individuals with promotion pride use eagerness means whereas individuals with prevention pride use vigilance means. Copyright © 2001 John Wiley & Sons, Ltd.
Exploring the multifaceted structure of sense of deprivation
Authors: Dar Yechezkel , Resh Nura 
Published: September 2002

AbstractDrawing from both social justice and deprivation research, we conceptualize expressions of sense of deprivation (equated with sense of injustice) as a three‐faceted structure defined by mode of experience, social reward, and social sphere of allocation. To empirically verify the fit between this conceptual structure and the actual configuration of people's deprivation reactions, we use a research model of two modes of experience (cognition and emotion), three classes of rewards (instrumental, relational and symbolic), and two social spheres of allocation (school and society at large). A Similarity Space Analysis (SSA) of 17 measures (that represents this model with data collected among Israeli adolescents) reproduced the three‐dimensional structure of sense of deprivation, although not all hypothesized affinities and distances between measures were empirically reconstructed. Copyright © 2001 John Wiley & Sons, Ltd.
Effects of supplemental viscous damping on inelastic seismic response of asymmetric systems
Authors: Goel Rakesh K. , Booker Cecilia A. 
Published: September 2002

AbstractThis paper investigates the effects of supplemental viscous damping on the seismic response of one‐storey, asymmetric‐plan systems responding in the inelastic range of behaviour. It was found that addition of the supplemental damping reduces not only deformation demand but also ductility and hysteretic energy dissipation demands on lateral load resisting elements during earthquake loading. However, the level of reduction strongly depends on the plan‐wise distribution of supplemental damping. Nearly optimal reduction in demands on the outermost flexible‐side element, an element generally considered to be the most critical element, was realized when damping was distributed unevenly in the system plan such that the damping eccentricity was equal in magnitude but opposite in algebraic sign to the structural eccentricity of the system. These results are similar to those noted previously for linear elastic systems, indicating that supplemental damping is also effective for systems expected to respond in the inelastic range. Copyright © 2001 John Wiley & Sons, Ltd.