bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruptive evolution: harnessing functional excess, experimentation, and science as tool
Authors: Felin Teppo , Kauffman Stuart 
Published: October 2023

AbstractWe explore the limitations of the adaptationist view of evolution and propose an alternative. While gradual adaptation can explain some biological and economic diversity, it cannot account for radical innovation (especially during the past 10,000 years). We argue that ubiquitously available but dormant “functional excess” provides the raw material for evolutionary disruptions. Harnessing this excess requires directed experimentation and what we call “protoscientific” problem solving. We highlight the implications of these arguments for evolutionary theory, including evolutionary economics and strategy.
The Relationship Between University Education and Pro-Immigrant Attitudes Varies by Generation: Insights From Japan
Authors: Kato Gento , Lu Fan 
Published: October 2023

Abstract
While there is lively debate on whether higher education cultivates support for immigrants in North America and Western Europe, there is little discussion on the extent to which the relationship generalizes beyond these continents. In light of Japan’s growing reliance on foreign workers, increase in university enrollment rates, as well as efforts to internationalize universities over the last half-century, we explore the relationship between university education and Japanese attitudes toward immigrants. Using two surveys asking an overlapping set of questions in 2009 and 2022, we find the relationship between university education and pro-immigrant attitudes varies by generation. Otherwise positive connections are significantly weakened for Japanese who entered universities in the 1990s through 2000s. Even though Japan is a modern democracy with well-developed higher education institutions, these institutions do not always correlate with more supportive attitudes toward immigrants. Our findings underscore the dynamic nature of higher education’s role in shaping public opinion outside of North America and Western Europe.
96 Impacts of Postweaning Dry Matter Intake Classification on Beef Heifer Performance and Cyclicity
Authors: Duncan Hattie I , Shike Daniel W 
Published: October 2023

Abstract
The objective was to evaluate the relationship of postweaning dry matter intake (DMI) classifications on performance and cyclicity in fall-born beef heifers. Heifers were classified based on DMI as either low (LoDMI; &lt; -0.5 SD from mean), average (AvgDMI; ± 0.5 SD from mean), or high (HiDMI; &gt; +0.5 SD from mean). Hifers (n = 147), either Angus or Hereford sired by commercial dams, were utilized in the analysis. Heifers were early weaned at 82 ± 7 days of age. Following weaning, heifers were managed in partially covered, drylot pens and fed a high-energy grain diet. At postweaning intake evaluation initiation (day 1), heifers were 170 ± 22 kg and 156 ± 7 days of age. Heifer DMI was determined over 56 days using GrowSafe system, and weights were collected every 14 days. Following the postweaning intake evaluation, heifers rotationally grazed endophyte-infected tall fescue paddocks. During the 166-day grazing period, heifers were weighed and assigned a hair shedding score monthly. Heifers returned to drylot pens on day 223 for a prebreeding feed efficiency evaluation on a high-forage diet. Foot scores were assigned on day 1 and 237. Heat detection patches were used to determine percentage of heifers cycling before synchronization. Cyclicity data were analyzed using GLIMMIX and all other response variables were analyzed using the MIXED procedure of SAS 9.4. By design, postweaning DMI differed (P &lt; 0.01) between treatments (5.5, 6.2, and 6.9 kg/d for LoDMI, AvgDMI, and HiDMI, respectively). Average daily gain (ADG) differed (P &lt; 0.01) during the postweaning evaluation period, with LoDMI being least, AvgDMI intermediate, and HiDMI greatest. On days 1 and 56, body weights (BW) differed (P &lt; 0.01); LoDMI were lightest, AvgDMI were intermediate, and HiDMI were heaviest. However, gain to feed during the postweaning evaluation period was not different (P = 0.33) by DMI classification. During the grazing period, BW differed (P &lt; 0.01) on days 84 and 141; HiDMI were heavier than LoDMI and AvgDMI. On day 113, BW differed (P &lt; 0.01); LoDMI were lightest, AvgDMI were intermediate, and HiDMI were heaviest. However, ADG and final grazing BW did not differ (P ≤ 0.12) during the grazing period by DMI classification. Hair shredding score was greater (P ≤ 0.04) for LoDMI than HiDMI on days 56, 84, and 113. Foot scores differed (P = 0.02) on day 1; LoDMI was less than AvgDMI and HiDMI. However, they only tended (P = 0.08) to be different on day 237. Percent cycling prior to synchronization did not differ (P = 0.25) by classification. In summary, LoDMI heifers performed poorer during postweaning evaluation than HiDMI heifers. However, grazing performance and cyclicity was similar among DMI classifications.
20 How do Producers and Nutritionists Determine Which Levers to Pull to Optimize Feed Cost and Profitability Under Differing Economic Conditions?
Authors: Kellner Trey A , Pilcher Chad M 
Published: October 2023

Abstract
Numerous factors including geopolitical unrest, supply chain disruption, government policies, and hyperinflation have resulted in a global increase in the cost of feed ingredients. Pork producers and swine nutritionists continuously answer the tricky question of how to best manage feed costs to maximize profitability under an everchanging economic landscape. Applied nutritionists must design and implement feeding programs that take into account and balance several key nutrition levers including energy, SID lysine to energy ratio, SID amino acid to lysine ratios, and feed processing along with factors including ingredient prices, pork price, barn space, feed mill throughput, and pig livability. Biological and economical models are developed and utilized to assist with prediction of key performance and profitability outcomes leveraging results from published and unpublished controlled research trials along with historical production data. The objective of this presentation is to provide insights into the decision-making process for feeding program design and implementation under current and theoretical economic situations.
179 Identification of Regulatory Mutations Affecting Complex Traits
Authors: Coutinho Luiz Lehmann 
Published: October 2023

Abstract
Understanding genetic bases controlling complex traits of economic importance is fundamental to improve breeding programs. Recent efforts from FAANG consortium resulted in significant progress in the identification of regulatory regions in farm animals. However, most studies in cattle have focused on Bos taurus, and limited information is available for Bos indicus. Considering that Bos indicus is the predominant subspecies in tropical regions, the objective of this work was to identify regulatory variants associated with meat quality by combining information from whole genome sequence (WGS), gene expression (RNA-seq), and open chromatin regions (ATAC-seq). For genome wide association study (GWAS), an imputed panel of SNPs was obtained from WGS of 26 sires, a Bovine HD 770K panel of 374 progenies, and SNPs called from RNA-seq data of Longissimus thoracis muscle (LT) of 192 progenies. The SNPs were identified using GATK4, and the dataset included almost 4.5 million variants that were pruned to 553,581 tag-SNPs (PLINK, r2 = 0.8). ATAC-seq data was obtained from duplicated samples of LT collected from two animals. Phenotype data included back fat thickness (BFT), intramuscular fat (IMF), ribeye area (REA) and shear force (SF). Association of genotypes (553,581 SNPs) and gene expression (12,991 genes), performed in Matrix eQTL resulted in 36,916 cis-eQTL and 14,408 trans-eQTL (FDR &lt; 0.05). For GWAS analysis of the eQTLs with phenotypic traits, we used a linear model in PLINK program (considering population stratification, contemporary group and hot carcass weight). GWAS resulted in 24 eQTLs associated with IMF, three with BFT, 12 with REA, and eight with SF (FDR &lt; 0.10). ATAC-seq analysis (using nfcore/atacseq pipeline implemented in Nextflow) resulted in 33,734 consensus peaks with a mean size of 2.4 kb. The overlap analyses among GWAS, eQTL, and ATAC-seq positions resulted in two, four and one putative regulatory regions that control gene expression and are associated with BFT, IMF and SF, respectively. Functional studies are still necessary to establish if identified mutations are the causal mutation. In conclusion, integration of GWAS, RNAseq, and ATAC-Seq data can result in identification of regulatory mutations associated with phenotypes of economic importance.
63 Biological and Management Factors Affecting Colostrum Intake and Pre-Weaning Survival in Piglets
Authors: Jenkins Abigail K , Flowers William L 
Published: October 2023

Abstract
A total of 581 piglets from 45 litters were used in an observational study to evaluate the relative importance of sow, litter, birth, and individual piglet characteristics on colostrum consumption and its association with nursing behaviors and pre-weaning survival. Sows were monitored continuously throughout parturition with farrowing and birth characteristics being recorded for each piglet. Nursing observations and skin temperatures for piglets were taken every 15 to 30 minutes after the onset of farrowing until sows expelled their placentae, after which, observations occurred hourly for the next four hours and then at 24 and 36 hours post farrowing. At approximately 36 hours post farrowing, blood samples were taken from piglets to determine immunocrit levels and estimate colostrum consumption by piglets. Nine variables entered the multiple regression model at P&lt; 0.15 and accounted for over 58% of the total variation observed for immunocrits. Colostrum consumption was least (P &lt; 0.0403) in piglets from litters with more than 16 total pigs born and from sows that averaged less than 10 pigs born alive in previous parities. Piglets that weighed more than 1.6 kg; spent less than 60 minutes in utero during farrowing; and nursed the 5 most anterior pairs of teats after farrowing had increased (P&lt; 0.0632) immunocrits that were 0.060 or greater (P = 0.0005) and a pre-weaning survival of 88.9 + 1.3%. Conversely, being born in a litter with more than 16 total born; with a birth weight of less than 1.2 kg; and lethargic increased (P&lt; 0.0413) the risk of piglets dying. On the day of farrowing, survival was least (P &lt; 0.0001) for piglets whose skin temperatures decreased below 30oC; took more than 60 minutes to initiate nursing activity; and then suckled only one pair of teats infrequently (&lt; 50% of nursing bouts) from the time they were born until four hours after farrowing was complete. These results indicate that low birth weight, lethargic, and chilled piglets were the most at-risk for low colostrum consumption and increased pre-weaning mortality. Piglets that began nursing quickly and frequently after birth and nursed the anterior and middle teat pairs, had the greatest immunocrits and survival.
69 Monoglycerides of Butyric, Caprylic, and Capric Fatty Acids Promote Intestinal Integrity and Host Defenses in Health-Challenged Weanling Pigs
Authors: White Cameron S , Hung Chien-Che , Lanka Saraswathi , Maddox Carol W , Sokale Adebayo O , Barri Adriana , Dilger Ryan N 
Published: October 2023

Abstract
Frequent incidence of post-weaning enterotoxigenic Escherichia coli (ETEC) diarrhea in the swine industry contributes to high mortality rates and associated economic losses. In this study, the effects of butyric, caprylic, and capric monoglycerides (MG) were investigated as a way to promote intestinal integrity and host defenses in weanling pigs infected with ETEC. A total of 160 pigs of equal sex were allotted to treatment groups based on body weight and sex. Throughout the 17-d study, three treatment groups were maintained: sham-inoculated pigs fed a control diet (NC, n = 40), ETEC-inoculated pigs fed the same control diet (PC, n = 60), and ETEC-inoculated pigs fed the control diet supplemented with the test article included at 3% of the diet (Test, n = 60). Following a 7-d adaptation period, pigs were orally inoculated on each of 3 consecutive days with either 3 mL of a sham-control (saline) or live ETEC culture (3 × 109 CFU/mL). The first day of inoculation was designated as 0 d post-inoculation (DPI), and all study outcomes reference this time-point. Fecal, tissue, and blood samples were collected from 48 individual pigs (NC, n = 12; PC and Test, n = 18) on 5 and 10 DPI for analysis of dry mater (DM), bacterial enumeration, inflammatory markers, and a practical assessment of intestinal permeability. All data were analyzed by ANOVA using the MIXED procedure of SAS. During the peak infection period (5 DPI) the NC group had greater (P &lt; 0.05) fecal DM and lower (P &lt; 0.05) rectal temperatures, total bacteria, total E. coli, and total F18 ETEC. By 9 DPI, the NC and Test groups had numerically decreased lactulose:mannitol ratios in blood plasma compared with the PC group, which indicated possible benefits to intestinal barrier function. Overall, MG supplementation did not appear to influence differences in fecal outcomes that were altered by ETEC infection, but some benefits in intestinal integrity were observed.
44 Identification of Vaginal Gene Expression Differences in Gilts Achieving Early or Late Puberty
Authors: Dierking Shannon L , Morton Jodi , Gonda Michael , Rekabdarkolaee Hossein , Clapper Jeffrey A , Levesque Crystal L 
Published: October 2023

Abstract
A known predictor of reproductive success in swine breeding herds is age at puberty. Early puberty is associated with improved long-term reproductive performance. Additionally, it has been established that vaginal gene expression in gilts changes throughout reproductive development. The hypothesis was that vaginal gene expression was correlated with estrus expression and therefore likelihood of reproductive success. Pre-pubertal gilts (n = 29) were followed from 70 d of age until first estrus or 215 d of age. Vaginal epithelia were collected using a swabbing technique at five key timepoints during reproductive development [70 (on farm arrival), 100 (mid-folliculogenesis), 130, 160 d of age, and first estrus or end of trial]. Boar exposure began on 160 d of age with 15 minutes of fenceline contact at 0700 h daily with trained technicians observing for signs of standing estrus (i.e., erect ears, swollen, pink vulva, allowing back pressure). By 215 d of age, 19 females were classified as expressing estrus early (160 to 181 d), 4 were average (181 to 202), and 6 were late or as anestrus (202 to 215 d). A subset of 5 gilts that expressed estrus early and 5 gilts that expressed estrus late or as anestrus were used for further investigation of vaginal gene expression. Total RNA was isolated from vaginal samples at 3 timepoints (100, 130, and 160 d of age). Samples were sent to a commercial genomic sequencing laboratory for RNA sequencing. Each gene was scaled individually so that all genes were on the same scale for comparison, and the Welch two sample t-test was applied to determine statistical differences. A total of 53 genes were differentially expressed between the early estrus and late estrus or anestrus groups. Data were further screened to identify genes that were expressed in at least 3 gilts in either the early estrus or late estrus group at a given time point; this subset of 26 genes were selected for direct comparison at each time point. At 100 d of age, late estrus gilts had increased expression (P = 0.05) of a gene commonly found in females with polycystic ovary syndrome. At 130 d of age, genes associated with the innate immune response had greater relative expression (P &lt; 0.05) in gilts expressing early estrus. At 160 d of age, genes associated with fat cell deposition had greater relative expression (P &lt; 0.05) in the early estrus expression gilts. Distinct differences in transcripts during reproductive development may be putative biomarkers for detection of early estrus in pre-pubertal females.
48 Evaluation of Branched-Chain Amino Acid Interactions in 13 to 23 kg Nursery Pigs Using a Central Composite Design
Authors: Humphrey Dalton C , Haydon Keith D , Greiner Laura L 
Published: October 2023

Abstract
Two groups of 240 pigs (PIC 337 X 1050, PIC Genus, Hendersonville, TN) with initial body weight (BW) 13.20 ± 3.05 kg were used to investigate interactions between the branched-chain amino acids (BCAA). At weaning, pigs were placed into 40 pens with three barrows and three gilts per pen and fed a common diet for 3 weeks. On day 21 post-weaning, pens were randomly assigned to 1 of 15 dietary treatments arranged in a rotatable central composite design. Experimental diets were formulated to various levels of standardized ileal digestible (SID) leucine, isoleucine, and valine by replacing corn starch and glycine with L-leucine, L-isoleucine, and L-valine in the base diet. Levels of BCAA were expressed as ratios to SID lysine and ranged from 98 to 180 for Leu, 46 to 64 for Ile, and 51 to 78 for Val. Diets were formulated to be sub-limiting in lysine at 1.19% SID Lys and exceed all other essential amino acid requirements. Additionally, diets were formulated to be isocaloric and isonitrogenous and met or exceeded NRC (2012) vitamin and mineral requirements. Pen weights and feed intake were measured for the 21-day experiment to calculate daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (G:F). Data were fit as a second-order response surface model according to the central composite design in R version 4.2.1 (R Core Team, 2022). The model included linear and quadratic effects of Leu, Ile, and Val, and their cross-products. Pen was the experimental unit, and variables were considered significant at P ≤ 0.10. Valine linearly and quadratically impacted ADG and G:F, regardless of Leu and Ile levels. There was an interaction between Leu and Ile for ADG (P = 0.079) and G:F (P = 0.031), where, at increasing levels of Leu, decreasing Ile improved ADG and G:F. Conversely, ADG and G:F decreased as both Leu and Ile increased. Isoleucine did not impact ADFI (P ≥ 0.458); however, there was an interaction between Leu and Val (P = 0.088), where Leu negatively impacted ADFI at low levels of Val, but did not influence ADFI at greater levels of Val. In conclusion, the response of nursery pig ADG and G:F to Val is independent of Leu and Ile levels, while ADG and G:F are reduced at greater levels of Leu and Ile, which is resolved as either Leu or Ile are reduced. Furthermore, at deficient levels of Val, ADFI is negatively impacted by increased Leu; however, the negative impacts of increasing Leu on ADFI are negated when Val is adequate or above requirement. This experiment emphasizes the complexity of amino acid metabolism in nursery pigs and the importance of understanding potential interactions among amino acids when conducting research.
192 Effect of Dietary Anhydrous Calcium Chloride in Early and Late Finishing on Pig Performance and Body Composition
Authors: Stowater Jay A , Johnson Colin , Gabler Nicholas K , Lonergan Steven M 
Published: October 2023

Abstract
Understanding how to control pig growth is critical during market disruptions, including foreign animal disease and harvest facility shutdowns. In times of severe market disruptions, feeding acidogenic salts such as anhydrous calcium chloride to suppress appetite is an effective way to manage pig growth. Therefore, the aim of this study was to understand the impact of feeding calcium chloride during early and late growth stages on grow-finish growth and body composition of pigs. Barrows and gilts (n = 240; 52 ± 6.9 kg BW) were allotted by sex across 60 pens and to one of three treatments fed in 3 diet phases (Phase 1, d 1-28; Phase 2 d 28-56; Phase 3 d 57-87). Treatment included: 1) Control corn-soy diets in phases 1-3, 2) 3% CaCl2 intervention in phase 1, control diet fed in phases 2 and 3 (IP1), and 3) Control diet fed in phases 1 and 2, and 3% CaCl2 intervention in phase 3 (IP3). All diets were formulated to meet or exceed amino acid and energy requirements and pigs were fed ad libitum. Pig BW were recorded at the start and end of each phase, and pen feed disappearance was recorded to calculate pen ADG, ADFI, and G: F. Loin eye area and fat depth were measured using real-time ultrasound at the end of phases 1 and 3. Data were analyzed with pen as the experimental unit and treatment, gender, and their interaction as fixed effects. No noteworthy gender by diet interactions were demonstrated and only the diet treatment, overall performance, and ultrasound trait means are reported (Table 1). Compared with the Control and IP3, IP1 reduced ADFI and ADG by 42% and 60%, respectively, in phase 1 (P&lt; 0.05). Feed efficiency, loin eye area, and fat depth were also reduced in the IP1 compared with the Control and IP3 pigs in phase 1 (P&lt; 0.05). In phase 2, the IP1 pigs did not have a significant difference in feed intake and their ADG increased by 22% compared with the Control and IP3 pigs (P&lt; 0.05) and therefore were more feed efficient. Implementation of CaCl2 in phase 3, the IP3 pigs reduced ADFI, ADG, and G: F compared with the control and IP1. Further, phase 3 loin eye area and fat depth were 13% and 17% less, respectively, in the IP3 pigs compared with the control. Overall, the IP3 treatment had the most severe reductions in ADG, ADFI, and feed efficiency (P&lt; 0.05). Early intervention (IP1) also attenuated overall G: F, ADFI, and ADG compared with the Control (P&lt; 0.05). These results demonstrate the efficacy of calcium chloride in attenuating pig feed intake and growth performance during early and late finishing.
5 DPP Lecture: Investigating the Impact of Weaning and Biological Sex on Gut Development: Discovering New Targets for Gut Inflammation
Authors: Moeser Adam J 
Published: October 2023

Abstract
Early life stress is known to have a profound impact on the development and function of the gut and immune system in various species, including pigs. In particular, the process of weaning is a significant source of stress in pigs and has been shown to negatively affect gut health and immunity. This presentation aims to examine the current knowledge on the impact of early life stress, particularly weaning, on gut development and immunity in pigs with a focus on chronic inflammation as a driver of disease risk. The presentation will also compare the immune system and gut development between female and male castrates, highlighting the influence of biological sex on gut health. Additionally, new data on potential targets for gut inflammation will be presented. The findings of this presentation will be analyzed in the context of pig production and the implications for enhancing gut health and immunity throughout production. Overall, this presentation will provide a comprehensive overview of the current understanding of the impact of early life stress on gut development and immunity in pigs and highlights key areas for future research.