bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of Department of Veterans Affairs community‐based outpatient clinics in enhancing rural access to geriatrics telemedicine specialty care
Authors: Pimentel Camilla B. , Dryden Eileen M. , Nearing Kathryn A. , Kernan Laura M. , Kennedy Meaghan A. , Hung William W. , Riley Jessica , Moo Lauren R. 
Published: November 2023

AbstractBackgroundHalf of the 4.7 M veterans who reside in rural communities and rely on U.S. Department of Veterans Affairs (VA) health care are older (≥65). Their rurality presents unique challenges, including a shortage of clinicians skilled in geriatric medicine. Community‐based outpatient clinics (CBOCs) help extend VA's geographic reach but are typically located in under‐resourced settings. Telemedicine may increase access to care, but little is known about CBOCs' capacity to leverage telemedicine to meet older patients' needs. We identified organizational barriers and facilitators to the use of geriatric telemedicine specialty care from the perspective of rural clinicians and staff.MethodsFrom February–April 2020, we interviewed CBOC clinicians and staff (N = 50) from 13 rural CBOCs affiliated with four VA Geriatric Research Education and Clinical Centers. Semi‐structured interviews addressed patient population characteristics; CBOC location, staffing, and in‐house resources; use of VA specialty care services; and telemedicine use. We developed a codebook using an iterative process and Gale's Framework Method thematically organize and analyze data.ResultsRespondents perceived that their CBOCs serve a predominantly older patient population. Four characteristics enabled CBOCs to offer geriatric telemedicine specialty care: partnerships with larger VA Medical Center teams; social worker/telehealth clinical technician knowledge of geriatrics and telehealth resources; periodic outreach/education from geriatric specialists; and routine use of other telehealth services. Barriers included: constraints on clinic space and unstable internet for telemedicine visits; staffing challenges leading to limited familiarity with telemedicine resources; and clinician and staff perceptions of older veterans' preference for in‐person visits.ConclusionsTelemedicine is an important modality to enhance access to care for an increasingly older and medically complex patient population. Although rural CBOCs provide a large portion of care to VA's growing geriatric population, staff are insufficiently trained in geriatrics, work in resource‐poor settings, and are largely unaware of VA telemedicine programs designed to support them.
Relationship between masseter muscle activity during wakefulness and temporomandibular disorder‐related symptoms
Authors: Maeda‐Iino Aya , Osako Yuki , Nakagawa Shoko , Takahashi Kotaro , Oga Yasuhiko , Furukawa‐Sainoki Minami , Harada Marina , Fukushima Mika , Miyawaki Shouichi 
Published: November 2023

AbstractBackgroundMasseter muscle activity during wakefulness may be associated with temporomandibular disorder (TMD)‐related symptoms, psychosocial status and pain‐related disability; however, this relationship is unclear.ObjectivesThis study aimed to determine the relationship between masseter muscle electromyography (EMG) burst/duration during wakefulness and TMD‐related symptoms, psychosocial status and pain‐related disability.MethodsSixty participants were assessed masseter muscle activity during wakefulness using a data‐logger‐type ultraminiature EMG system and TMD‐related symptoms, psychosocial status and pain‐related disability through Axis I and II of the diagnostic criteria for TMD (DC/TMD). EMG bursts lasting longer than 0.25 s but less than 2.0 s and those lasting longer than 2.0 s were classified as phasic and tonic bursts, respectively.ResultsParticipants with palpation‐related pain in the temporalis and masseter muscles, as assessed through the DC/TMD examination form in Axis I, had more bursts (number/h) (p = .035 and p = .009, respectively) and longer duration (time/h) (p = .013 and p = .004, respectively) of tonic bursts of the masseter muscle during wakefulness. Participants with palpation‐related pain in the masseter muscles had higher oral behaviour scores during wakefulness using Axis II (p = .001), which affected the number and duration of tonic bursts of the masseter muscle activity during wakefulness (p = .011 and p = .007, respectively).ConclusionAs tonic bursts mainly reflect clenching, individuals with pain in the masseter muscles by palpation may have a high frequency and longer duration of clenching, as well as a high frequency of oral behaviours during wakefulness.
Simian immunodeficiency virus and storage buffer: Field-friendly preservation methods for RNA viral detection in primate feces
Authors: Wilde Tessa H. C. , Shukla Rajni Kant , Madden Christopher , Vodovotz Yael , Sharma Amit , McGraw W. Scott , Hale Vanessa L. 
Published: November 2023

ABSTRACT

Wild non-human primates carry many types of RNA viruses, including simian immunodeficiency virus (SIV), simian foamy virus, simian T-cell leukemia virus, and hepatitis C virus. These viruses can also infect humans via zoonotic transmission through handling and consumption of primate bushmeat. Characterizing viral prevalence and shedding in natural hosts is critical to understand infection and transmission risks within and between primate species. Here, we sought to identify a robust “field-friendly” method (i.e., without freezing or refrigeration) for preserving viral RNA, specifically SIV, in primate fecal samples. Fecal samples were collected from a mantled guereza colobus (
Colobus guereza
) housed at the Columbus Zoo and Aquarium. Samples were homogenized and inoculated with three concentrations (low, medium, and high) of inactivated SIV and preserved in four different storage buffers (DNA/RNA Shield, RNA
later
, 95% Ethanol, and Viral Transport Medium). SIV viral RNA was then extracted from samples at four time points (1 week, 4 weeks, 8 weeks, and 12 weeks) to determine the efficacy of each buffer for preserving SIV RNA. Quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used for the detection and quantification of viral RNA. At all concentrations, DNA/RNA Shield yielded the highest average SIV virion concentrations. We then successfully validated this approach using fecal samples from known SIV-positive and SIV-negative sooty mangabeys (
Cercocebus atys
) housed at Emory National Primate Research Center. Our results indicate that DNA/RNA Shield is an optimal “field-friendly” buffer for preserving SIV RNA in fecal samples over time and may also be effective for preserving other RNA viruses in feces.

IMPORTANCE
Simian immunodeficiency virus (SIV), which originated in African monkeys, crossed the species barrier into humans and ultimately gave rise to HIV and the global HIV/AIDS epidemic. While SIV infects over 40 primate species in sub-Saharan Africa, testing for RNA viruses in wild primate populations can be challenging. Optimizing field-friendly methods for assessing viral presence/abundance in non-invasively collected biological samples facilitates the study of viruses, including potentially zoonotic viruses, in wild primate populations. This study compares SIV RNA preservation and recovery from non-human primate feces stored in four different buffers. Our results will inform future fieldwork and facilitate improved approaches to characterizing prevalence, shedding, and transmission of RNA viruses like SIV in natural hosts including wild-living non-human primates.
Single-cell atlas reveals characteristic changes in intrahepatic HBV-specific leukocytes
Authors: Pan Banglun , Wang Zengbin , Chen Rui , Zhang Xiaoxia , Qiu Jiacheng , Wu Xiaoxuan , Yao Yuxin , Luo Yue , Wang Xiaoqian , Tang Nanhong 
Published: November 2023

ABSTRACT

In the progression of liver disease, various metabolic disorders and inflammatory factors cause dysfunction of intrahepatic leukocytes, but there is no research to summarize the changes in the liver microenvironment driven by different pathological factors, especially HBV. Therefore, this study attempted to explore the inherent laws of disease-driven microenvironment changes in diseased livers. Using mass cytometry, flow cytometry, and immunofluorescence, we analyzed the changes in intrahepatic leukocytes from mouse liver diseases and patients with hepatocellular carcinoma (HCC) in this study. We noticed that (i) the inhibitory effect of hepatitis B virus (HBV) on intrahepatic leukocytes did not increase with the surface antigen titers compared with the low hepatitis B surface antigen concentration group, intrahepatic T cells from the high one showed higher expression of PD-1 and lower expression of TIM-3 and CTLA-4, so the proportion of exhausted T cells decreased in the high one, which contributed to the tolerance or adaptation of the liver to HBV; (ii) leukocytes in HBV-associated HCC were in a highly dysfunctional state with highest PD-1/TIM-3/CTLA-4/TOX; (iii) the detailed leukocyte ratio was appropriate to assess the prognosis of HCC, the ratio of cytotoxic/naive/effector/central memory CD8
+
T cells to CD8
+
T cells was positively correlated with the overall survival. In conclusion, our phenotype analysis deeply revealed the function differences in intrahepatic leukocytes caused by different liver disease factors, highlighting their unique changes in HBV related HCC, and helping to discover new HCC targeted immunotherapy strategies.

IMPORTANCE

Hepatitis B virus (HBV)-specific CD8
+
T cells play a central role in the clearance of virus and HBV-related liver injury. Acute infection with HBV induces a vigorous, multifunctional CD8
+
T cell response, whereas chronic one exhibits a weaker response. Our study elucidated HBV-specific T cell responses in terms of viral abundance rather than the timing of infection. We showed that in the premalignant stage, the degree of impaired T cell function was not synchronized with the viral surface antigen, which was attributed the liver’s tolerance to the virus. However, after the development of hepatocellular carcinoma, T cell exhaustion was inevitable, and it was marked by the exhaustion of the signature transcription factor TOX.
Evaluation of tissue- and plasma-derived tumor mutational burden (TMB) and genomic alterations of interest in CheckMate 848, a study of nivolumab combined with ipilimumab and nivolumab alone in patients with advanced or metastatic solid tumors with high TMB
Authors: He Jie , Kalinava Natallia , Doshi Parul , Pavlick Dean C , Albacker Lee A , Ebot Ericka M , Tukachinsky Hanna , Pratt James , Fusaro Gina , Oxnard Geoffrey R , Green George , Fabrizio David , Baden Jonathan 
Published: November 2023

BackgroundAn accumulation of somatic mutations in tumors leads to increased neoantigen levels and antitumor immune response. Tumor mutational burden (TMB) reflects the rate of somatic mutations in the tumor genome, as determined from tumor tissue (tTMB) or blood (bTMB). While high tTMB is a biomarker of immune checkpoint inhibitor (ICI) treatment efficacy, few studies have explored the clinical utility of bTMB, a less invasive alternative for TMB assessment. Establishing the correlation between tTMB and bTMB would provide insight into whether bTMB is a potential substitute for tTMB. We explored the tumor genomes of patients enrolled in CheckMate 848 with measurable TMB. The correlation between tTMB and bTMB, and the factors affecting it, were evaluated.MethodsIn the phase 2 CheckMate 848 (NCT03668119) study, immuno-oncology-naïve patients with advanced, metastatic, or unresectable solid tumors and tTMB-high or bTMB-high (≥10 mut/Mb) were prospectively randomized 2:1 to receive nivolumab plus ipilimumab or nivolumab monotherapy. Tissue and plasma DNA sequencing was performed using the Foundation Medicine FoundationOne CDx and bTMB Clinical Trial Assays, respectively. tTMB was quantified from coding variants, insertions, and deletions, and bTMB from somatic base substitutions. Correlations between tTMB and bTMB were determined across samples and with respect to maximum somatic allele frequency (MSAF). Assay agreement and variant composition were also evaluated.ResultsA total of 1,438 and 1,720 unique tissue and blood samples, respectively, were obtained from 1,954 patients and included >100 screened disease ontologies, with 1,017 unique pairs of tTMB and bTMB measurements available for assessment. Median tTMB and bTMB were 3.8 and 3.5 mut/Mb, respectively. A significant correlation between tTMB and bTMB (r=0.48, p<0.0001) was observed across all sample pairs, which increased to r=0.54 (p<0.0001) for samples with MSAF≥1%. Assay concordance was highest for samples with MSAF≥10% across multiple disease ontologies and observed for both responders and non-responders to ICI therapy. The variants contributing to tTMB and bTMB were similar.ConclusionsWe observed that tTMB and bTMB had a statistically significant correlation, particularly for samples with high MSAF, and that this correlation applied across disease ontologies. Further investigation into the clinical utility of bTMB is warranted.
Effect of sodium sulfate concentration in drinking water for beef heifers, and the in vitro effect of bismuth subsalicylate on H2S production and fiber disappearance
Authors: Evans M.G. , Ribeiro G.O. , Henry D.H. , Johnson J.A. , Campbell J.R. , Waldner C. , Penner G.B. 
Published: November 2023

This study evaluated the effects of ( i) increasing the concentration of sulfate in drinking water on dry matter intake (DMI), water intake, ruminal fermentation, and apparent total-tract digestibility, and ( ii) water sulfate concentration and bismuth subsalicylate (BSS) dose on in vitro ruminal fermentation. Eight ruminally cannulated beef heifers (382 ± 45 kg) were used in a replicated incomplete 3 × 3 Latin square design. Water treatments contained low (342 ± 29 mg/L; LS), medium (2785 ± 72 mg/L; MS), or high (4948 ± 163 mg/L; HS) sulfate. Ruminal digesta was collected to evaluate water sulfate and BSS dose (0.0%, 0.2%, 0.4%, and 0.6% dry matter) on ruminal H2S production in vitro. Water intake increased linearly as water sulfate concentration increased ( p = 0.002), but DMI was not affected. Heifers drinking MS and HS had greater ruminal H2S at 10.5 h after water provision than LS (sulfate × time, p < 0.001). In vitro H2S production (µg and µg/mL of rumen inoculum) increased and plateaued with increasing sulfate ( p < 0.001) and was linearly reduced ( p < 0.001) by increasing BSS dose. Increasing water sulfate concentrations did not negatively affect water or feed intake but increased ruminal H2S concentrations. Bismuth subsalicylate may reduce H2S production.
Impact of quintessence and cloud of strings on self-consistent d-dimensional charged thin-shell wormholes
Authors: Waseem Arfa , Javed Faisal , Gul M. Zeeshan , Mustafa G. , Errehymy Abdelghani 
Published: November 2023

AbstractThis article evaluates the stability constraints of higher-dimensional geometry of thin-shell wormholes developed from the two equivalent copies of inner and outer d-dimensional charged anti-de Sitter black holes bounded by a cloud of strings and quintessence. Such geometrical structures are built using a cut-and-paste method that joins two identical forms of black hole solutions at the hypersurface. We develop the equation of motion for the constructed wormholes and then use the linear radial perturbation approach to examine the stable configuration. The stability constraints depend on the dimensions of the black holes, cloud, and quintessence parameters. It is worth mentioning that the possibility of a stable structure is greatest for the choice of d-dimensional charged anti-de Sitter black holes with quintessence and a cloud of strings.
SHORT-MATURITY ASYMPTOTICS FOR OPTION PRICES WITH INTEREST RATE EFFECTS
Authors: PIRJOL DAN , ZHU LINGJIONG 
Published: November 2023

We derive the short-maturity asymptotics for option prices in the local volatility model in a new short-maturity limit [Formula: see text] at fixed [Formula: see text], where r is the interest rate and q is the dividend yield. In the case of practical relevance [Formula: see text] being small, however, our result holds for any fixed [Formula: see text]. The result is a generalization of the Berestycki–Busca–Florent formula ( Berestycki et al. , 2002 ) [Asymptotics and calibration of local volatility models, Quantitative Finance 2, 61–69] for the short-maturity asymptotics of the implied volatility which includes interest rates and dividend yield effects of [Formula: see text] to all orders in n. We obtain the analytical results for the ATM volatility and skew in this asymptotic limit. Explicit results are derived for the CEV model. The asymptotic result is tested numerically against the exact evaluation in the square-root model [Formula: see text], which demonstrates that the new asymptotic result is in very good agreement with the exact evaluation in a wide range of model parameters relevant for practical applications.
XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies
Authors: Patel Ekta , Malkova Natalia V. , Crowe David , Pederzoli-Ribeil Magali , Fantini Damiano , Fanny Manoussa , Madala Hanumantha Rao , Jenkins Kurt A. , Yerov Oleg , Greene Justin , Guzman Wilson , O'Toole Caitlin , Taylor Jacob , O'Donnell Rebekah K. , Johnson Parker , Lanter Bernard B. , Ames Brian , Chen Jia , Vu Sallyann , Wu Hsin-Jung , Cantin Susan , McLaughlin Megan , Hsiao Yu-Shan S. , Tomar Dheeraj S. , Rozenfeld Raphael , Thiruneelakantapillai Lakshmanan , O'Hagan Ronan C. , Nicholson Benjamin , O'Neil Jennifer , Bialucha Carl Uli 
Published: November 2023

Abstract
IL12 is a proinflammatory cytokine, that has shown promising antitumor activity in humans by promoting the recruitment and activation of immune cells in tumors. However, the systemic administration of IL12 has been accompanied by considerable toxicity, prompting interest in researching alternatives to drive preferential IL12 bioactivity in the tumor. Here, we have generated XTX301, a tumor-activated IL12 linked to the human Fc protein via a protease cleavable linker that is pharmacologically inactivated by an IL12 receptor subunit beta 2 masking domain. In vitro characterization demonstrates multiple matrix metalloproteases, as well as human primary tumors cultured as cell suspensions, can effectively activate XTX301. Intravenous administration of a mouse surrogate mXTX301 demonstrated significant tumor growth inhibition (TGI) in inflamed and non-inflamed mouse models without causing systemic toxicities. The superiority of mXTX301 in mediating TGI compared with non-activatable control molecules and the greater percentage of active mXTX301 in tumors versus other organs further confirms activation by the tumor microenvironment–associated proteases in vivo. Pharmacodynamic characterization shows tumor selective increases in inflammation and upregulation of immune-related genes involved in IFNγ cell signaling, antigen processing, presentation, and adaptive immune response. XTX301 was tolerated following four repeat doses up to 2.0 mg/kg in a nonhuman primate study; XTX301 exposures were substantially higher than those at the minimally efficacious dose in mice. Thus, XTX301 has the potential to achieve potent antitumor activity while widening the therapeutic index of IL12 treatment and is currently being evaluated in a phase I clinical trial.
Darolutamide Added to Docetaxel Augments Antitumor Effect in Models of Prostate Cancer through Cell Cycle Arrest at the G1–S Transition
Authors: Buck Stefan A.J. , Van Hemelryk Annelies , de Ridder Corrina , Stuurman Debra , Erkens-Schulze Sigrun , van ’t Geloof Sem , Teubel Wilma J. , Koolen Stijn L.W. , Martens-Uzunova Elena S. , van Royen Martin E. , de Wit Ronald , Mathijssen Ron H.J. , van Weerden Wytske M. 
Published: November 2023

Abstract
Resistance to taxane chemotherapy is frequently observed in metastatic prostate cancer. The androgen receptor (AR) is a major driver of prostate cancer and a key regulator of the G1–S cell-cycle checkpoint, promoting cancer cell proliferation by irreversible passage to the S-phase. We hypothesized that AR signaling inhibitor (ARSi) darolutamide in combination with docetaxel could augment antitumor effect by impeding the proliferation of taxane-resistant cancer cells. We monitored cell viability in organoids, tumor volume, and PSA secretion in patient-derived xenografts (PDX) and analyzed cell cycle and signaling pathway alterations. Combination treatment increased antitumor effect in androgen-sensitive, AR-positive prostate cancer organoids and PDXs. Equally beneficial effects of darolutamide added to docetaxel were observed in a castration-resistant model, progressive on docetaxel, enzalutamide, and cabazitaxel. In vitro studies showed that docetaxel treatment with simultaneous darolutamide resulted in a reduction of cells entering the S-phase in contrast to only docetaxel. Molecular analysis in the prostate cancer cell line LNCaP revealed an upregulation of cyclin-dependent kinase inhibitor p21, supporting blockade of S-phase entry and cell proliferation. Our results provide a preclinical support for combining taxanes and darolutamide as a multimodal treatment strategy in patients with metastatic prostate cancer progressive on ARSi and taxane chemotherapy.
Jellyfish search algorithm for economic load dispatch under the considerations of prohibited operation zones, load demand variations, and renewable energy sources
Authors: Trong Hien Chiem , Nguyen Thuan Thanh , Nguyen Thang Trung 
Published: November 2023

<span lang="EN-US">This paper suggests a modified version of the former economic load dispatch (MELD) problem with the integration of wind power plant (WPP) and solar power plants (SPP) into thermal units (TUs). The target of the whole study is to cut the total producing electricity cost (TPEC) as much as possible. Three meta-heuristic algorithms, including particle swarm optimization (PSO), jellyfish search (JS) and salp swarm algorithm (SSA), are applied to solve the MELD. The real performance of these optimization tools is tested on the first system with six thermal units considering prohibited zones, and the second system with the combination of the first system and one solar, and two WPPs. In addition, the variation of load demand in 24 hours per day is also taken into account in the second system. JS is proved to be the most effective method for dealing with MELD. Furthermore, JS can also reach lower or the same TPEC as other previous algorithms. Hence, JS is a recommended to be a strong computing method for dealing with the MELD problem. </span>
Entrevista com Lourenço Mutarelli
Authors: Mutarelli Lourenço , Mendes Neto Joaquim Ferreira 
Published: November 2023

Lourenço Mutarelli nasceu em 18 abril de 1964, em São Paulo. É escritor, quadrinista, ator. Como quadrinista, escreveu e ilustrou obras como A caixa de areia (2006) e a trilogia de Diomedes, reunida em volume único em 2012. Seu primeiro romance, O cheiro do ralo (2002), viraria filme em 2007, do qual Mutarelli participa como personagem. Depois viriam os romances O natimorto (2004) , Jesus Kid I (2004), A arte de produzir efeito sem causa (2008), Miguel e seus demônios (2009), Nada me faltará (2010), O grifo de Abdera (2015), O filho mais velho de Deus e/ou o livro IV (2018). Nesta entrevista, Lourenço Mutarelli fala de seu nono romance, O livro dos mortos, que será publicado no segundo semestre de 2022. A entrevista foi realizada durante a pandemia, via comunicação online, no dia 13 de março de 2022.