bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating Oviposition Deterrent, Larvicidal and Repellent Effects of Different Plant Extracts and Their Combinations against Aedes aegypti
Authors: Majeed Shahid , Ishaq Amna , Javaid Rameen , Sharif Rao Muhammad Sajjad , Iftikhar Sana , Akbar Rabia , Rahman Abdur 
Published: October 2023

Abstract
Mosquitoes are significantly important in public health as they transmit many vector-borne diseases like dengue fever (DF), malaria, and filariasis to millions of people worldwide. Although there is no effective vaccination for the prevention of dengue, avoiding mosquito bites is one method to control mosquito populations. Due to the extensive uses of synthetic repellent,’ mosquitoes established resistance against them. The plant-based extracts are used as an alternative to targeted insecticides. Therefore, current study aimed to examine the oviposition-deterrent, larvicidal and repellent efficacy of plant-based extracts Clove (Syzygium aromaticum), Cinnamon (Cinnamomum verum), Lemon (Citrus limon), Orange (Citrus sinensis), Onion (Allium cepa), Garlic (Allium sativum), Lemon grass (Cymbopogon citratus), and Aloe vera (Aloe barbadensis Miller) against Aedes aegypti under laboratory conditions. The maximum repellency was observed by clove, and lemon peel plant extract compared to all other botanical plants. However, lemon, clove, garlic, onion, and garlic with lemongrass are promising for oviposition control. While, clove, lemon peel, orange and lemon peels, and lemongrass combination for larvicidal control. In terms of repellents, the most suspected repellent plants showed by the combinations of (Olive oil, lemon peel, citrus peel, lemongrass, Aloe vera), (Olive oil, lemon peel, citrus peel, lemongrass), (Olive oil, lemon peels, citrus peels, Aloe vera), such as 19.85, 19.85,19.84 repellency value along with 100% protection time up to 1 h. The establishment of plant-based products against Aedes aegypti, complemented with insecticidal activity, could lead to an innovative approach to managing mosquito populations and disease vectors.
Modelling impacts to water quality in salmonid-bearing waterways following the introduction of Emerald Ash Borer in the Pacific Northwest, USA.
Authors: Maze Dominic , Bond Julia , Mattsson Monte 
Published: October 2023

Abstract

Oregon ash (Fraxinus latifolia Benth.) wetlands and riparian forests are an important economic, cultural, and ecological resource in the Pacific Northwest, USA, and are threatened by the invasive insect, emerald ash borer (Agrilus planipennis Fairmmaire) (EAB). Following the discovery of EAB in Forest Grove, Oregon in June of 2022, concern has focused on EAB-induced ash mortality that has the potential to alter vegetation communities and modify wetland hydrology by elevating the water table. Of primary concern is an increase in solar loading of waterways and wetlands that are already degraded beyond meeting their beneficial uses for Endangered Species Act-listed salmonids and other cold-water species following canopy dieback of Oregon ash. Our study, begun before the first detection of EAB on the West Coast, models potential impacts of EAB-mediated Oregon ash canopy loss to temperature-related water quality on two waterways in the vicinity of Portland, Oregon. Our results indicate a significant increase in solar loading with likely negative impacts to aquatic Endangered Species Act-listed salmonids, other aquatic wildlife, and associated habitat. We forecast greater impacts to these resources outside our study scope and include management considerations and recommendations for entities with water quality-related regulatory obligations.
The Identification and Characterization of a New Species, Neopestalotiopsis Strain CP1, a Pathogen Causing Leaf Blight on Curcuma phaeocaulis in Guangxi, China
Authors: Li Shanshan , Long Meimei , Guo Lulu , Luo Yaqi , Sun Xianchao , Qian Kun , He Lin , Ma Guanhua 
Published: October 2023

Abstract
Zedoary turmeric (Curcuma phaeocaulis), a cornerstone of traditional Chinese medicines in Guangxi Province, holds immense significance. Regrettably, the emergence of a novel leaf wilt in October 2017 has cast a pall over its production, leading to detrimental impacts on both yield and quality. By meticulous single-conidial isolation, this new pathogen was successfully extracted from ten diseased leaves. Subsequent confirmation of pathogenicity was achieved via the meticulous execution of Koch's postulates. In pursuit of accurate identification, morphological data were harmonized with a concatenated sequence analysis of the rDNA internal transcribed spacer (ITS), β-tubulin (TUB), and translation elongation factor 1 (TEF) regions. This rigorous approach unveiled the pathogen as an entirely novelNeopestalotiopsis species, warranting the nomenclature Neopestalotiopsis strain CP1. The optimum pH of conidial germination was between 5.0 and 5.5. Among the 665 different metabolites of the Neopestalotiopsis strain CP1, 60 carbon sources, 35 nitrogen sources, 4 phosphate sources, 8 sulfur sources, and 1 biometabolic pathway, effectively promoted the growth and development of conidia. The optimum pH of conidial germination was between 5.0 and 5.5. Among the 665 different metabolites of the Neopestalotiopsis strain CP1, 60 carbon sources, 35 nitrogen sources, 4 phosphate sources, 8 sulfur sources, and 1 biometabolic pathway, effectively promoted the growth and development of conidia. The ramifications of this pathogen's definitive identification and ongoing surveillance cannot be overstated, signifying a pivotal alert to zedoary turmeric cultivators in confronting this newfound peril.
Estimating the prevalence of symptomatic and asymptomatic dengue fever among adults in the Central Health Region - Burkina Faso: a protocol for field study
Authors: Ouédraogo Jean Claude Romaric Pingdwindé , Ilboudo Sylvain , Compaoré Tegwinde Rebeca , Tougma Alix , Bado Prosper , Nitiéma Mathieu , Zeba Mohamadi , Ouédraogo Wendlasida Thomas , Ouédraogo Salfo , Lougué Siaka , Tarnagda Grissoum , Kini Bondo Félix , Ouédraogo Sylvin , Savadogo Léon Gueswendé Blaise 
Published: October 2023

Abstract
Background: Dengue fever is misdiagnosed as malaria in Africa and underreported. In Burkina Faso, specific data on dengue fever are lacking on adults. This study aims to estimate the prevalence of symptomatic and asymptomatic dengue fever and to assess their determinants among adults over two years in the Central Health Region of Burkina Faso.
Methods: An analytical cross-sectional study will be conducted with repetitive quantitative data collection in 2022 and 2023. It will include adults suspected of dengue from health facilities of the 5 districts - Central Health Region, and adult contacts of dengue probable cases from households. Participants will undergo rapid diagnosis tests for dengue fever and malaria, with dengue-positive cases to be confirmed by Real-Time Polymerase Chain Reaction. The main objectives are: i) To compare the prevalence of symptomatic dengue fever among adults suspected of dengue between 2022 and 2023. ii) To compare the prevalence of symptomatic and asymptomatic dengue fever among contact adults between 2022 and 2023. iii) To evaluate the sociodemographic and bio-clinical determinants of symptomatic dengue fever among suspected cases. iv) To evaluate the sociodemographic, bio-clinical, and environmental determinants of asymptomatic dengue fever among the contacts.
Conclusions: This study will generate comprehensive data on symptomatic and asymptomatic dengue fever among adults in Burkina Faso. Such data will drive strategies in responding to dengue epidemics in the country.
The impact of elevated sulfur and nitrogen levels on cadmium tolerance in Euglena species
Authors: Saville Barry J. , Kennedy Victoria , Kaszecki Emma , Donaldson Michael E. 
Published: October 2023

Abstract
Heavy metal (HM) pollution threatens human and ecosystem health. Current methods for remediating water contaminated with HMs are expensive and have limited effect. Therefore, bioremediation is being investigated as an environmentally and economically viable alternative. Freshwater protists Euglena gracilis and Euglena mutabilis were investigated for their tolerance to cadmium (Cd). A greater increase in cell numbers under Cd stress was noted for E. mutabilis but only E. gracilis showed an increase in Cd tolerance following pre-treatment with elevated concentrations of S or N. To gain insight regarding the nature of the increased tolerance RNA-sequencing was carried out on E. gracilis. This revealed transcript level changes among pretreated cells, and additional differences among cells exposed to CdCl2. Gene ontology (GO) enrichment analysis reflected changes in S and N metabolism, transmembrane transport, stress response, and physiological processes related to metal binding. Identifying these changes enhances our understanding of how these organisms adapt to HM polluted environments and allows us to target development of future pre-treatments to enhance the use of E. gracilis in bioremediation relating to heavy metals.
The study of the relationship between moderate to severe Sleep Obstructive Apnea and cognitive impairment, anxiety, and depression
Authors: HONG Yanan , PEI Chong , HAO Lingli , XU Kang , LIU Feifei , DING Zhen 
Published: October 2023

Abstract

Objective
The present study endeavored to investigate the interconnection between obstructive sleep apnoea (OSA) and cognitive function, alongside the manifestations of depression and anxiety. Simultaneously, an analysis was conducted to discern the factors exerting influence upon cognitive function.
Methods
A cohort of 102 patients, who had undergone polysomnography (PSG) at Binhu Hospital, Anhui Medical University, between January 2022 and June 2023, was encompassed in the study. Employing the PSG findings, these individuals were classified into two distinct categories: the grouping consisted of those with either negligible or mild OSA, and the other comprised individuals with moderate to severe OSA. Utilizing the Montreal Cognitive Assessment (MoCA-Beijing), Stroop Color and Word Test (SCWT), Digit Span Test (DST), Self-rating Depression Scale (SDS), and Self-rating Anxiety Scale (SAS), scores were recorded and analysed for each of the respective assessments. Additionally, discrepancies and associations between these groups were also scrutinised.
Results
The group exhibiting moderate to severe OSA demonstrated significantly elevated measurements in parameters such as neck circumference, BMI, completion times for SCWT-A, B, C, Sleep Inefficiency Index (SIE), SAS, and SDS, in comparison to the no or mild OSA group. Furthermore, the moderate-severe OSA group manifested notably diminished MoCA scores in areas of visual-spatial and executive function, memory, language, abstraction, delayed recall, orientation, total MoCA score, lowest oxygen saturation (LSaO2), average oxygen saturation, DST-b, and DST-f, as contrasted with the no-mild OSA group. These inter-group disparities exhibited statistical significance (P < 0.05). The MoCA total score portrayed inverse correlations with age, Apnea-Hypopnea Index (AHI), BMI, SIE, SAS, SDS, CT90%, AHT90%, and Hypoxic Apnea Duration (HAD) (ranging from − 0.380 to -0.481, P < 0.05). Conversely, it displayed positive correlations with DST-f, DST-b, LSaO2, and average oxygen saturation (ranging from 0.414 to 0.744, P < 0.05). Neck circumference, AHI, and SAS were autonomously linked to MoCA scores (OR = 1.401, 1.028, 1.070, P < 0.05), whilst AHI exhibited an independent correlation with SDS and SAS scores (OR = 1.001, P = 0.003).
Conclusion
Patients grappling with moderate to severe OSA frequently reveal cognitive impairment and concomitant emotional predicaments encompassing depression and anxiety. These manifestations share an intimate association with AHI, LSaO2, and average oxygen saturation. Notably, anxiety, when coupled with OSA, emerges as an autonomous influential element impinging upon cognitive impairment.
PLGA fine nanoparticle for drug delivery systems: experimental and simulation
Authors: MOHAMMADIKHAH RASOOL , ABOLGHASEMI HOSSEIN , RASHIDI ALI-MORAD , ESMAEILI MOHAMMAD 
Published: October 2023

Abstract
In this work, PLGA copolymers/polymers with high molecular weight (up to 100000) become synthesised using ring opening method to be suitable for drug delivery systems. Two drugs namely (Tamoxifen citrate, cisplatin) were encapsulated into nanoPLGA copolymer capsules. Drugs were differently loaded according to their solvability (i.e. hydrophilic or hydrophobic status). After characterisation copolymer 75:25 PLGA was the best sample because of DSC analysis showing. One of PLGA copolymer (75:25 PLGA) was candidate to play the role of capsule for drug loading process that has more stability and maximum half time in the in-vivo environment. This work presents all steps to get new cancerthrap nanoparticles, from copolymer synthesis to drug encapsulation. Copolymer/polymer with molecular weight above 100000 which is very expensive and rare as a result was synthesised. Average diameter of nanoparticles shows 40–60 nm to promote much drug loading into encapsulating process, with the help of double emulsion method. Characteristics tests like TGA, DSC, DLS, SEM and TEM are accomplished to verify average size distribution as key factor and other parameters. Some key parameters to reduce size such as surfactant type are investigated. Simulations equipped by high performance computers are done which are strong devices to reproduce experimental results with low relative error. Maximum relative error is below 13 ℅. Double micro emulsion technique was successful to drug encapsulation in nano scale as we do in this work. The novelty comes from very fine nano capsules production and high loading drug in comparison with other works. Molecular dynamics simulation was accomplished by FORTRAN handy coding (with parallel computations) to give us a superior understanding of micelle formation, critical micelle concentration and size distribution or other critical parameters. Both simulation results and experimental data agree whether qualitatively and quantitatively. Results agree with experimental data and also with others reported results.
Differences in proportions and characteristics of malignant tumours by lung lobe
Authors: Nilssen Yngvar , Brustugun Odd Terje , Fjellbirkeland Lars , Helland Åslaug , Møller Bjørn , Wahl Sissel Gyrid Freim , Solberg Steinar 
Published: October 2023

Abstract
Background
The main focus on the characteristics of malignant lung tumours has been the size, position within the lobe, and infiltration into neighbouring structures. The aim of this study was to investigate the distribution and characteristics of malignant tumours between the lung lobes and whether the diagnosis, treatment, and outcome differed based on location.
Methods
This study is based on 10,849 lung cancer patients diagnosed in 2018–2022 with complete data on the location and characteristics of the tumours. The proportions of tumours in each lobe divided by its volume were termed the relative proportion.
Results
The right upper lobe comprised 31.2% of the tumours and 17.6% of the lung volume. The relative proportion of 1.77 was higher than in the other lobes (p < 0.001). The right middle lobe had a relative proportion of 0.64 but the highest proportion of neuroendocrine tumours (26.1% vs 15.3 on average). Surgical resection was more often performed in patients with tumours in the lower lobes, and curative radiotherapy was more often performed in the upper lobes. After adjusting for age, sex, stage, and histology, the location of the tumour was found to be a significant independent predictor for resection but not for survival.
Conclusion
The main finding of the right upper lobe as a site of predilection for lung cancer is similar to tuberculosis and pneumoconiosis. This may be explained that most of the inhaled air, containing bacilli, inorganic particles or tobacco smoke goes to the upper and right parts of the lung.
Microsurgical denervation of the spermatic cord with tunica vaginalis fenestration: results of a case series with a review of the literature
Authors: Almekaty Khaled , AbdelRahman Islam , Moubasher Amr , Abdel-Raheem Tarek , Zeidan Ashraf , Raheem Amr Abdel 
Published: October 2023

Abstract
Microsurgical denervation of the spermatic cord (MDSC) is one of the lines of management of chronic orchialgia, but it has not been fully adopted by different centers. This article reviews a case series experience with a review of the literature. Eighteen patients (18 testicular units) with chronic orchialgia, unresponsive to medical treatment were prospectively enrolled in this study. All underwent unilateral MDSC with a slight modification in the technique (tunica vaginalis fenestration). Efficacy of the technique was evaluated by the degree of pain improvement using the Visual Analogue Scale (VAS) which was done preoperatively and 6 months postoperatively. Safety was assessed by reporting any intra- or post-operative complications. Available literature was reviewed looking at safety and efficacy of the technique. Six patients had complete pain resolution, 11 had partial but significant resolution of their pain and the pain remained the same in one patient following MDSC. The VAS pain score significantly improved from a median (IQR) of 8(7.25-9) to 2(2-4.75) postoperatively (p=0.0029). No significant complications have been encountered in this study. So, MDSC seems to be a safe and efficacious surgical option for the treatment of resistant chronic scrotal pain (CSP) and should be more widely adopted by specialized centers.
Improving Attitudes Toward Trauma-Informed Care in the Neonatal Intensive Care Unit Through Comprehensive Multi-Disciplinary Education
Authors: Davis Patricia , Hubbard Dena , Gladdis Tiffany , Nitkin Christopher , Hansen Kara , Keith-Chancy Erin , Godwin Jennifer , Staggs Vincent , Babbar Shilpa , Hardy Michelle , Ashbaugh Jacqulin , Carter Brian 
Published: October 2023

Abstract

Objective: This study measured staff understanding and integration of trauma-informed care following comprehensive education.
Study Design: This mixed method design used the validated Attitudes Related to Trauma-Informed Care (ARTIC) scale and open-ended survey questions via REDCap optional surveys. Trauma-informed care education was made available to staff members in a level IV NICU. Pre- and post-intervention ARTIC scores were compared and post-intervention REDCap surveys were analyzed.
Result: There were 245 multi-disciplinary NICU team members who completed the ARTIC survey before and/or after the educational intervention; and 764 REDCap surveys were completed throughout the study time. ARTIC scores increased from pre- to post-training both for participants with data at both time points (0.5 SD mean increase) and among those with data at only one time point (0.4 SD mean increase). Content analysis of the REDCap survey corroborated the ARTIC results.
Conclusion: System-wide trauma-informed education can achieve measurable effect in a NICU setting.
Efficacy and safety of individualized rituximab in proliferative lupus nephritis
Authors: Hao Yingxuan , Guo Yanhong , Yu Lu , Wang Liuwei , Zhai Zihan , Lin Tang 
Published: October 2023

Abstract
The dosage, efficacy and safety of Rituximab (RTX) in the treatment of proliferative lupus nephritis (PLN) have not been fully investigated. We collected the data episodes from January 2017 to July 2023 of patients with PLN who were hospitalized in the Department of Nephrology of the First Affiliated Hospital of Zhengzhou University and treated with RTX, and the renal remission, recurrence and adverse reactions after RTX treatment were analyzed retrospectively. A total of 22 patients were enrolled, including 7 patients who were treated as a naive and 15 relapsed/refractory patients, 4 patients required hemodialysis on admission. After the first treatment of RTX, a total of 21(95.45%) patients achieved B cell depletion, and a total of 15 patients (68.18%) achieved complete response (CR). The annual average frequency of relapse per individual in the maintenance remission period in 15 refractory/relapsing patients decreased from (0.494±0.346) times to (0.026±0.010) time (P＜0.001), and the maintenance doses of prednisone decreased from (14.17±9.39) mg/day [ 20.0（12.3，40.0）months] to (8.67±5.97) mg/d[ 12.0（10.0,15.0）months] (P=0.040). Infection occurred in 3 patient within the first 3 months after RTX treatment and recovered after treatment, 3 cases were removed from hemodialysis, and no patient died. In conclusion, RTX can effectively treat PLN, reduce frequency of recurrences and decrease the dose of glucocorticoids, without increasing the occurrence of increased infection risks.
Long-term outcomes of patients with embolic stroke of undetermined source according to subtype
Authors: Lee Il Hyung , Heo JoonNyung , Lee Hyungwoo , Jeong JaeWook , Kim Joon Ho , Han Minho , Yoo Joonsang , Kim Jinkwon , Baik Minyoul , Park Hyungjong , Kim Young Dae , Nam Hyo Suk 
Published: October 2023

Abstract

Background and Aims: The prognosis of patients with embolic stroke of undetermined source (ESUS) may vary according to the underlying cause. Therefore, we aimed to divide ESUS into subtypes and assess the long-term outcomes.
Methods: Consecutive patients with acute ischemic stroke who underwent a comprehensive workup, including transesophageal echocardiography and prolonged electrocardiography monitoring, were enrolled. We classified ESUS into minor cardioembolic (CE) ESUS, arteriogenic ESUS, two or more causes ESUS, and no cause ESUS. Arteriogenic ESUS was sub-classified into complex aortic plaque (CAP) ESUS and non-stenotic (<50%) relevant artery plaque (NAP) ESUS.
Results: A total of 775 patients were enrolled. During 1286 ± 748 days follow-up, 116 major adverse cardiovascular events (MACE) occurred (4.2 events/100 patient-years). Among the ESUS subtypes, CAP ESUS was associated with the highest MACE frequency (9.7/100 patient-years, p=0.021). Cox regression analyses showed that CAP ESUS was an independent predictor of MACE (hazard ratio 2.466, 95% confidence interval 1.305–4.660) and any stroke recurrence (hazard ratio 2.470, 95% confidence interval, 1.108–5.508).
Conclusions:The prognosis of ESUS varies according to the subtype, with CAP ESUS having the worst prognosis. Categorizing ESUS into subtypes could improve patient care and refine clinical trials.
Mechanisms of action of berberine hydrochloride in planktonic cells and biofilms of Pseudomonas aeruginosa
Authors: Liu Qingyu , Tang Yongxue , Jiang Siyu , Yu Xiao , Zhu Huibin , Xie Xiaobing , Ning Xingwang 
Published: October 2023

Abstract

The growing prevalence of extensive drug-resistant and pan-drug resistant Pseudomonas aeruginosa (P. aeruginosa) has become a significant concern for global public health, particularly due to the formation of biofilms. Consequently, it is crucial to explore the development of novel antimicrobials that specifically target P. aeruginosa and its biofilms. Here we found a natural compound named berberine hydrochloride, subsequently, Crystalline violet staining and Confocal laser scanning microscope further demostrated that berberine hydrochloride suppress biofilm. Additionally, it was found that the expression of pslA and other biofilm-related genes were downregulated. Compared with available antibiotics, berberine hydrochloride has multiple modes of action in P. aeruginosa. The antimicrobial mechanism showed that berberine hydrochloride might have antimicrobial effects by damaging bacterial cell membranes, generating reactive oxygen species (ROS) and lowering intracellular adenosine triphosphate (ATP). Futhermore, berberine hydrochloride possesses various attributes, including minimal cytotoxicity and reduced susceptibility to drug resistance. In a mice peritonitis model, it was reported to inhibit the growth of P. aeruginosa significantly and displayed high bacteriostatic action. Collectively, the berberine hydrochloride is a safe and effective antibacterial agent for inhibiting P. aeruginosa.