bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gingival-derived mesenchymal stem cell therapy regenerated the radiated salivary glands: functional and histological evidence in murine model
Authors: Zayed Hagar M. , Kheir El Din Nevine H. , Abu-Seida Ashraf M. , Abo Zeid Asmaa A. , Ezzatt Ola M. 
Published: February 2024

Abstract
Background
Radiotherapy in head and neck cancer management causes degeneration of the salivary glands (SG). This study was designed to determine the potential of gingival mesenchymal stem cells (GMSCs) as a cell-based therapy to regenerate irradiated parotid SG tissues and restore their function using a murine model.

Methods
Cultured isolated cells from gingival tissues of 4 healthy guinea pigs at passage 3 were characterized as GMSCSs using flow cytometry for surface markers and multilineage differentiation capacity. Twenty-one Guinea pigs were equally divided into three groups: Group I/Test, received single local irradiation of 15 Gy to the head and neck field followed by intravenous injection of labeled GMSCs, Group II/Positive control, which received the same irradiation dose followed by injection of phosphate buffer solution (PBS), and Group III/Negative control, received (PBS) injection only. Body weight and salivary flow rate (SFR) were measured at baseline, 11 days, 8-, 13- and 16-weeks post-irradiation. At 16 weeks, parotid glands were harvested for assessment of gland weight and histological and immunohistochemical analysis.

Results
The injected GMSCs homed to degenerated glands, with subsequent restoration of the normal gland histological acinar and tubular structure associated with a significant increase in cell proliferation and reduction in apoptotic activity. Subsequently, a significant increase in body weight and SFR, as well as an increase in gland weight at 16 weeks in comparison with the irradiated non-treated group were observed.

Conclusion
The study provided a new potential therapeutic strategy for the treatment of xerostomia by re-engineering radiated SG using GMSCs.
POSEIDON: Peptidic Objects SEquence-based Interaction with cellular DOmaiNs: a new database and predictor
Authors: Preto António J. , Caniceiro Ana B. , Duarte Francisco , Fernandes Hugo , Ferreira Lino , Mourão Joana , Moreira Irina S. 
Published: February 2024

AbstractCell-penetrating peptides (CPPs) are short chains of amino acids that have shown remarkable potential to cross the cell membrane and deliver coupled therapeutic cargoes into cells. Designing and testing different CPPs to target specific cells or tissues is crucial to ensure high delivery efficiency and reduced toxicity. However, in vivo/in vitro testing of various CPPs can be both time-consuming and costly, which has led to interest in computational methodologies, such as Machine Learning (ML) approaches, as faster and cheaper methods for CPP design and uptake prediction. However, most ML models developed to date focus on classification rather than regression techniques, because of the lack of informative quantitative uptake values. To address these challenges, we developed POSEIDON, an open-access and up-to-date curated database that provides experimental quantitative uptake values for over 2,300 entries and physicochemical properties of 1,315 peptides. POSEIDON also offers physicochemical properties, such as cell line, cargo, and sequence, among others. By leveraging this database along with cell line genomic features, we processed a dataset of over 1,200 entries to develop an ML regression CPP uptake predictor. Our results demonstrated that POSEIDON accurately predicted peptide cell line uptake, achieving a Pearson correlation of 0.87, Spearman correlation of 0.88, and r2 score of 0.76, on an independent test set. With its comprehensive and novel dataset, along with its potent predictive capabilities, the POSEIDON database and its associated ML predictor signify a significant leap forward in CPP research and development. The POSEIDON database and ML Predictor are available for free and with a user-friendly interface at https://moreiralab.com/resources/poseidon/, making them valuable resources for advancing research on CPP-related topics. Scientific Contribution Statement: Our research addresses the critical need for more efficient and cost-effective methodologies in Cell-Penetrating Peptide (CPP) research. We introduced POSEIDON, a comprehensive and freely accessible database that delivers quantitative uptake values for over 2,300 entries, along with detailed physicochemical profiles for 1,315 peptides. Recognizing the limitations of current Machine Learning (ML) models for CPP design, our work leveraged the rich dataset provided by POSEIDON to develop a highly accurate ML regression model for predicting CPP uptake.
Graphical Abstract
Effects of topical application of resveratrol on tight junction barrier and antimicrobial compound production in lactating goat mammary glands
Authors: Tsugami Yusaku , Nii Takahiro , Isobe Naoki 
Published: February 2024

AbstractIn mammary glands, the formation of less-permeable tight junctions (TJs) and the production of antimicrobial compounds like lactoferrin and defensins are important for preventing mastitis. Resveratrol, a polyphenol contained in red grapes, is known to protect mammary epithelial cells (MECs) from oxidative stress; however, oral administration of resveratrol causes a decrease in certain biological processes through conjugation and metabolic conversion. In this study, we determined the beneficial effects of resveratrol on TJs and antimicrobial compounds in cultured goat MECs by adding it to the medium, and in lactating goat mammary glands by topical application for percutaneous absorption. TJ barrier function was evaluated by transepithelial resistance and expression or localization pattern of claudins for culture model in vitro and by somatic cell count, Na+, albumin, and IgG in milk for topical application in vivo. Concentrations of antimicrobial compounds and cytokines were measured using ELISA. Activation of STAT3 was evaluated by Western blotting. Resveratrol strengthened TJ barrier function by upregulating claudin-3 in cultured MECs and topical application to udders reduced somatic cell count, Na+, albumin, and IgG in milk. Resveratrol increased β-defensin and S100A7 levels in cultured MECs and milk. In addition, resveratrol down-regulated cytokine production and STAT3 pathway. These findings suggest that the topical application of resveratrol to udders may be effective in preventing mastitis.
Genomic characteristics of cfr and fexA carrying Staphylococcus aureus isolated from pig carcasses in Korea
Authors: Kim Eiseul , Yang Seung-Min , Kwak Hyo-Sun , Moon Bo-Youn , Lim Suk-Kyung , Kim Hae-Yeong 
Published: February 2024

AbstractThe emergence of transferable linezolid resistance genes poses significant challenges to public health, as it does not only confer linezolid resistance but also reduces susceptibility to florfenicol, which is widely used in the veterinary field. This study evaluated the genetic characteristics of linezolid-resistant Staphylococcus aureus strains isolated from pig carcasses and further clarified potential resistance and virulence mechanisms in a newly identified sequence type. Of more than 2500 strains isolated in a prior study, 15 isolated from pig carcasses exhibited linezolid resistance (minimum inhibitory concentration ≥ 8 mg/L). The strains were characterized in detail by genomic analysis. Linezolid-resistant S. aureus strains exhibited a high degree of genetic lineage diversity, with one strain (LNZ_R_SAU_64) belonging to ST8004, which has not been reported previously. The 15 strains carried a total of 21 antibiotic resistance genes, and five carried mecA associated with methicillin resistance. All strains harbored cfr and fexA, which mediate resistance to linezolid, phenicol, and other antibiotics. Moreover, the strains carried enterotoxin gene clusters, including the hemolysin, leukotoxin, and protease genes, which are associated with humans or livestock. Some genes were predicted to be carried in plasmids or flanked by ISSau9 and the transposon Tn554, thus being transmittable between staphylococci. Strains carrying the plasmid replicon repUS5 displayed high sequence similarity (99%) to the previously reported strain pSA737 in human clinical samples in the United States. The results illustrate the need for continuous monitoring of the prevalence and transmission of linezolid-resistant S. aureus isolated from animals and their products.
Automatic classification of the physical surface in sound uroflowmetry using machine learning methods
Authors: Alvarez Marcos Lazaro , Arjona Laura , Iglesias Martínez Miguel E. , Bahillo Alfonso 
Published: February 2024

AbstractThis work constitutes the first approach for automatically classifying the surface that the voiding flow impacts in non-invasive sound uroflowmetry tests using machine learning. Often, the voiding flow impacts the toilet walls (traditionally made of ceramic) instead of the water in the toilet. This may cause a reduction in the strength of the recorded audio signal, leading to a decrease in the amplitude of the extracted envelope. As a result, just from analysing the envelope, it is impossible to tell if that reduction in the envelope amplitude is due to a reduction in the voiding flow or an impact on the toilet wall. In this work, we study the classification of sound uroflowmetry data in male subjects depending on the surface that the urine impacts within the toilet: the three classes are water, ceramic and silence (where silence refers to an interruption of the voiding flow). We explore three frequency bands to study the feasibility of removing the human-speech band (below 8 kHz) to preserve user privacy. Regarding the classification task, three machine learning algorithms were evaluated: the support vector machine, random forest and k-nearest neighbours. These algorithms obtained accuracies of 96%, 99.46% and 99.05%, respectively. The algorithms were trained on a novel dataset consisting of audio signals recorded in four standard Spanish toilets. The dataset consists of 6481 1-s audio signals labelled as silence, voiding on ceramics and voiding on water. The obtained results represent a step forward in evaluating sound uroflowmetry tests without requiring patients to always aim the voiding flow at the water. We open the door for future studies that attempt to estimate the flow parameters and reconstruct the signal envelope based on the surface that the urine hits in the toilet.
The global economic burden of COVID-19 disease: a comprehensive systematic review and meta-analysis
Authors: Faramarzi Ahmad , Norouzi Soheila , Dehdarirad Hossein , Aghlmand Siamak , Yusefzadeh Hasan , Javan-Noughabi Javad 
Published: February 2024

Abstract
Background
The COVID-19 pandemic has caused a considerable threat to the economics of patients, health systems, and society.

Objectives
This meta-analysis aims to quantitatively assess the global economic burden of COVID-19.

Methods
A comprehensive search was performed in the PubMed, Scopus, and Web of Science databases to identify studies examining the economic impact of COVID-19. The selected studies were classified into two categories based on the cost-of-illness (COI) study approach: top-down and bottom-up studies. The results of top-down COI studies were presented by calculating the average costs as a percentage of gross domestic product (GDP) and health expenditures. Conversely, the findings of bottom-up studies were analyzed through meta-analysis using the standardized mean difference.

Results
The implemented search strategy yielded 3271 records, of which 27 studies met the inclusion criteria, consisting of 7 top-down and 20 bottom-up studies. The included studies were conducted in various countries, including the USA (5), China (5), Spain (2), Brazil (2), South Korea (2), India (2), and one study each in Italy, South Africa, the Philippines, Greece, Iran, Kenya, Nigeria, and the Kingdom of Saudi Arabia. The results of the top-down studies indicated that indirect costs represent 10.53% of GDP, while the total estimated cost accounts for 85.91% of healthcare expenditures and 9.13% of GDP. In contrast, the bottom-up studies revealed that the average direct medical costs ranged from US $1264 to US $79,315. The meta-analysis demonstrated that the medical costs for COVID-19 patients in the intensive care unit (ICU) were approximately twice as high as those for patients in general wards, with a range from 0.05 to 3.48 times higher.

Conclusions
Our study indicates that the COVID-19 pandemic has imposed a significant economic burden worldwide, with varying degrees of impact across countries. The findings of our study, along with those of other research, underscore the vital role of economic consequences in the post-COVID-19 era for communities and families. Therefore, policymakers and health administrators should prioritize economic programs and accord them heightened attention.
Judgment, shame, and coercion: the criminal legal system and reproductive autonomy
Authors: Garcia-Alexander Ginny , Thompson Melissa 
Published: February 2024

Abstract
Background
A growing body of research has called attention to limitations to reproductive autonomy in both women who are socially disadvantaged and in those who have had contact with the criminal legal (CL) system. However, it is unclear whether CL system contact influences contraceptive use patterns and how these processes unfold. We utilize a mixed-methods approach to investigate whether history of arrest is associated with receipt of contraceptive counseling, use of long-term contraception, sterilization, and subsequent desire for reversal of sterilization. We further consider how agents in and around the CL system may influence women’s reproductive decisions and outcomes (856 survey respondents; 10 interviewees).

Results
We observe that women who have been arrested more commonly report receipt of contraceptive counseling and sterilization. They are also significantly more likely to want their sterilization reversed. Our in-depth interviews suggest that women with CL contact experience considerable shame, and in some cases, coercion to limit fertility from various agents in and outside the criminal legal system including medical providers, Parole/Probation Officers (POs), guards, and family members.

Conclusions
Our findings suggest the need for ongoing attention to how exposure to this system may promote uneven use of certain forms of contraception and dissatisfaction, i.e., desire for reversal of sterilization, among these women. Findings further suggest that de-emphasizing the CL system as a means through which to address reproductive needs should be considered.
Novel plasma-polymerized coating facilitates HeLa cell spheroid formation, exerting necroptosis via β-cyclodextrin-encapsulated resveratrol
Authors: Jang Sujeong , Baek Namwuk , Seo Youngsik , Lim Hyuna , Jung Donggeun , Park Heonyong 
Published: February 2024

AbstractBeta-cyclodextrins (β-CDs) comprise a pore for accommodating resveratrol (Res), thereby boosting its bioavailability. Res-incorporated β -CD (Res/CD) may be cytotoxic against both normal and cancer cells. Herein, we examined whether Res/CD exhibits anticancer activity against tumor spheroids, similar to in vivo tumor mass. To prepare three-dimensional spheroids, 1,1,1,3,5,7,7,7 octamethyl-3,5-bis(trimethylsiloxyl) tetrasiloxane (OMBTSTS) was deposited to the surface of the culture dish via plasma polymerization. We observed that HeLa cells grew as spheroids on the OMBTSTS-deposited surface at 20 W plasma power. Res/CD was delivered to the hypoxic core of the spheroid, inducing necrosis, whereas Res was not. Consistently, 10 μM Res alone was not cytotoxic to two-dimensional HeLa cells grown on a culture dish and three-dimensional spheroids. However, Res/CD promoted the necroptosis of spheroids, which were split into small fragments, ultimately inducing cell spheroid death. Collectively, our data suggest that nontoxic levels of Res/CD were efficiently delivered to the hypoxic core of tumor spheroids, promoting cell death. Therefore, Res/CD can be used as an effective anticancer drug. Moreover, the plasma-polymerized OMBTSTS modification technique provides insights into the efficient formation of spheroids in various cancer cell lines.
Students’ perspectives on the ‘STEM belonging’ concept at A-level, undergraduate, and postgraduate levels: an examination of gender and ethnicity in student descriptions
Authors: Dost Gulsah 
Published: February 2024

Abstract
Background
Women and ethnic minorities have historically been underrepresented in some STEM fields. It is therefore important to understand the factors influencing students’ persistence in STEM fields, and what STEM belonging means from the voices of socio-demographically diverse students, in order to ensure equity among students in STEM fields and to increase their belonging to this field, which has not been clearly defined in the literature, and there is a lack of agreement about the definition of belonging itself. For this purpose, the perspectives of students in England are brought together in this study in an attempt to better understand the concept of STEM belonging within a broader context of integration.

Result
The inductive thematic analysis with the voices of socio-demographically diverse 313 A-level, undergraduate and postgraduate Mathematics, Physics, and Chemistry students showed that compared to male students, it was mostly female, non-binary, non-White, and first-generation students who defined STEM belonging as ‘Feeling safe and comfortable in the STEM community and settings’. This theme was defined by the participants as the group/community/learning environment in which the individual belongs, the interaction with the people in the field, and the comfort that this participation/interaction creates. Students stressed the importance of creating a supportive and welcoming STEM environment so that individuals can feel at home, as well as a safe and comfortable STEM environment for people of all identities, genders, ethnicities, and backgrounds. Based on the participants’ responses, this study also conceptualised the concept of STEM belonging as having four phases: the ‘adaptation phase’, the ‘integration phase’, the ‘continuum phase’, and the ‘transition phase’. These four phases which comprise the STEM belonging concept are consecutive and interconnected.

Conclusion
The study concluded that all human beings are connected in a relational way (either strong or weak) and that the concept of STEM belonging develops as a result of interactions with ‘self’ and ‘others’ who have a shared passion and an interest in STEM fields. Although individuals have intrinsic motivation and individual prompts in STEM fields (i.e. resilience, beliefs in their capacity/ability and curiosity, etc.), social determinants (i.e. receiving adequate support from members of the STEM community, social capital and social cohesion, etc.) also play a significant role in influencing individual’s sense of STEM belonging.
Ultraviolet resistance on weft knitted fabric coated by benzotriazoles
Authors: Nguyen Tuan Anh , Nguyen Nu Nhu Tram 
Published: February 2024

AbstractUltraviolet resistance upon four different types of weft knitted fabrics including Lacoste, Single Jersey, Rib and Interlock were experimentally measured and evaluated by ultraviolet–visible spectroscopy under effects of gauge, specific weight, fiber composition and finishing agent as benzotriazole derivatives. The results showed that ultraviolet protection factor (UPF) of Interlock sample reached the highest value at 42.7. Simultaneously, the higher gauge of Single Jersey knitted fabrics was, the better its ultraviolet protection exhibited. Also, insertion of polyester fibers or spandex fibers into cotton knitted fabrics with a certain ratio strongly increased UPF values. Notably, all UPFs of coated fabrics with a given content of benzotriazole were significantly improved and almost coated samples could undergo several washing cycles and still retain their inherent breathability. This work demonstrated that cotton knitted fabrics coated with benzotriazoles were excellently enhanced ultraviolet protection. Especially, the UPF values on Interlock or Rib specimens as well as Single Jersey samples blended with polyester/spandex fibers were found to be higher than 15.
Chinese social value change and its relevant factors: an age-period-cohort effect analysis
Authors: Haiyan Gao , Peng Wang , Tam Tony 
Published: February 2024

AbstractUsing data from the World Values Survey (1990–2012), this article attempts to describe and analyze the changing trends in public values during social transformations in China. It is found that public attitudes toward authority tend to be increasingly favorable over time, values in the private sphere tend to be more open, while postmaterialist values show a trend of first rising and then falling. The most salient feature of the cohort effect is that the generations born after the reform and opening-up in 1978 identify more closely with the value of respect for authority, while their recognition of postmaterialist values tends to decline. The change in values is related to China's modernization process, global cultural diffusion, and important historical events and is also deeply influenced by the two-way interaction between tradition and modernity.
Exploring the potential role of decentralised biogas plants in meeting energy needs in sub-Saharan African countries: a techno-economic systems analysis
Authors: Robin Thomas , Ehimen Ehiaze 
Published: February 2024

AbstractTo achieve the Sustainable Development Goals by 2030, low-income sub-Saharan African countries urgently need to electrify. Biogas production from anaerobic digestion could make a contribution to a solution to improved electricity generation and access in these regions. This study evaluates its feasibility using Malawi as a case study. The aim is to provide households with a continuous supply of gas for cooking and electricity. The study examines different sizes of fixed dome reactors (3, 6, 12 m3) and assumes individual household ownership of 2, 4, and 6 cows. Several feedstocks and conditions are considered, such as cow dung alone, co-generation of cow dung with human faeces, cow dung with grass, and cow dung with maize residue. The economic benefits of selling biogas and fertilisers are calculated, and the cost of construction for different sizes of reactors is determined. Results show that co-generation of cow dung and grass silage in the reactor of 12 m3 with six cows has a positive net present value (NPV) of $8962, while for a small farm with a 6 m3 reactor capacity, co-digestion of cow dung with maize residue is preferable. The feasibility of the technology depends heavily on current national economic conditions, such as inflation, electricity prices, and construction material costs. A sensitivity analysis estimated that a 25% increase in the cost of electricity could increase the net present value (NPV) from − $3345 to $1526 for the generation of biogas from cow dung alone. Overall, this technology could have a significant impact on the lives of low-income households in sub-Saharan Africa by improving their access to electricity and providing a source of income through the sale of biogas and digestate.