bluuebunny/crossref_metadata_2025_split huggingface dataset:
S1P Lyase Inhibition Increased Intestinal S1P, Disrupted the Intestinal Barrier and Aggravated DSS-Induced Colitis
Authors: Kaur Prabhdeep , Karuppuchamy Thangaraj , Chilukuri Amruth , Kim Margaret , Urrete Josef , Shen Zining , Saxon Leo , Lundborg Luke R , Mikulski Zbigniew , Jedlicka Paul , Rivera-Nieves Jesús 
Published: February 2025

Abstract

Background
Sphingosine-1-phospate (S1P) receptor agonists (eg, ozanimod) desensitize migrating lymphocytes by irreversibly binding to S1P receptors (S1PR) and triggering their proteasomal degradation. Desensitized lymphocytes cannot sense S1P, therefore, halting lymphocyte recirculation. The S1P lyase (SPL) irreversibly degrades S1P and its inhibition disrupts the S1P gradient. We previously found that systemic SPL inhibitors induce central immunosuppression. Here, we examined whether SPL inhibition may attenuate colitis without systemic immunotoxicity.

Methods
We first analyzed SPL expression and localization in mice using qRT-PCR and immunohistochemistry. SPL inhibitors 4-deoxypyridoxine hydrochloride (DOP) and 2-acetyl-4-(tetrahydroxybutyl) imidazole (THI) were used to inhibit SPL systemically, whereas a conditional intestinal epithelial cell (IEC)-specific SPL-deficient mouse was used to evaluate the effects of IEC-specific SPL inhibition on survival, disease activity, histological severity of dextran sulfate sodium-induced colitis, S1P levels, and intestinal permeability.

Results
Sgpl1 mRNA transcripts and protein were ubiquitously expressed in gastrointestinal (GI) tract leukocytes and IEC. Systemic SPL inhibitors did not induce colitis by themselves but depleted CD4+ and CD8+ T cells from blood. However, contrary to its therapeutic effects on ileitis, systemic inhibition reduced survival, accelerated weight loss, worsened histopathological inflammation indices, and tissue damage. We then examined the effects of IEC-specific inhibition on peripheral cell counts and severity of colitis. We found that while it spared peripheral immunity, it similarly hastened colitis. Finally, we examined whether colitis acceleration was due to epithelial barrier compromise after disruption of the S1P gradient. We found that not only systemic but also IEC-specific SPL inhibition increased local S1P levels and led to IEC barrier compromise.

Conclusion
Homeostatic intestinal S1P levels are critical for the regulation of IEC barrier function. Further studies using adaptive immunity-based inflammatory bowel diseases (IBD) models are required to assess the translational value of IEC-specific SPL inhibition as a therapeutic target for human IBD.
Gender, democracy, and the legal academy: Afterword to the Foreword by Gráinne de Búrca, Rosalind Dixon, and Marcela Prieto Rudolphy
Authors: Chan Cora 
Published: February 2025

Abstract
This Afterword reflects on the Foreword’s three key contributions—proof of a gender gap in the legal academy, revelation of the problem’s complexity, and an exploration of solutions—from the perspective of a woman scholar specializing in constitutional law in Hong Kong, a former British colony and now a special administrative region of China. While Hong Kong is an international financial center with a strong higher education sector, and its government is explicitly committed to gender equality, the territory is steeped in traditional Chinese values and culture and has undergone a sharp authoritarian turn since the protest movement in 2019. It therefore provides an interesting vantage point from which to assess issues of gender in the legal academy. In particular, this Afterword cites and provides data substantiating the existence of a gender gap in Hong Kong’s legal academy, highlights the intersectional challenges arising from authoritarianism, marketization, and gender as well as other forms of marginalization, and underscores the relationship between gender equality and democratic governance.
Vendor managed inventory under price-and-stock-dependent demand, buyer’s space limitations and preservation investment
Authors: Sen Nabin , Modak Indrani , Giri Bibhas C , Bardhan Sudarshan 
Published: February 2025

Abstract
Accepted by: M. Zied Babai
This study explores a two-level vendor managed inventory model for deteriorating items facing demand dependent on stock and price, while operating under a consignment stock policy. It presents a joint vendor-buyer model that considers varying deterioration rate and preservation technology investment for both parties. The model is developed under the constraint of limited warehouse space at the buyer’s end. The study offers an analytical discourse on the model that aims to determine the optimal values for the selling price, preservation technology investment and shipment time. An assessment is conducted to determine the optimal model for a specific business through a comparative analysis of the capacitated and uncapacitated models. The applicability and robustness of the proposed model are established through the inclusion of numerical illustration and sensitivity analysis. The proposed model is deemed suitable for industries that specializes in clothing, food and pharmaceutical products, provided that all underlying assumptions of the model are adequately met.
A Comparative Analysis of Universal and Sentinel Surveillance Data for COVID-19: Insights from Argentina, Chile, and Mexico (2020–2022)
Authors: Redondo-Bravo Lidia , Zureick Kinda , Voto Carla Jimena , Molina Avendaño Xaviera , Flores-Cisneros Laura , Fowlkes Ashley , Iummato Luciana Eva , Giovacchini Carlos Maria , Olivares Barraza Maria Fernanda , Rodriguez Ferrari Paula , Gutiérrez-Vargas Rosaura , Zaragoza-Jiménez Christian Arturo , García-Rodríguez Gabriel , López-Gatell Hugo , Rodriguez Angel , Couto Paula , Rondy Marc , Vicari Andrea 
Published: February 2025

Abstract

Background
In 2020, countries implemented universal surveillance to detect and monitor SARS-CoV-2 cases. Although crucial for early monitoring efforts, universal surveillance is resource intensive. To understand the implications of transitioning from universal to sentinel surveillance for monitoring SARS-CoV-2 transmissibility, morbidity and mortality, and disease seriousness, we compared measures of SARS-CoV-2 reported from both surveillance strategies in Argentina, Chile, and Mexico.

Methods
We obtained weekly case counts in Argentina, Chile, and Mexico, in periods when both universal and sentinel surveillance were ongoing. To assess the countries’ surveillance strategies, we measured the proportion of total sites that were included in sentinel surveillance. We compared eight measures of SARS-CoV-2 transmissibility, morbidity and mortality, and disease seriousness between sentinel and universal surveillance and assessed the correlation between the two strategies for the eight measures. Pearson's and Spearman's correlation was classified as very strong (r(s)=0.8–1.0), strong (r(s)=0.60–0.79), moderate (r(s)=0.50–0.59), or poor (r&lt;0.50).

Results
The proportion of total sites included in sentinel surveillance was 5.8% for Argentina, 1.1% for Chile, and 7.6% for Mexico. A total of 21 measures were calculated (8 for Mexico, 8 for Chile, and 5 for Argentina). Of these, 17 showed consistency between the two surveillance strategies, with strong or very strong correlations (r=0.66–0.99): all 8 measures for Mexico, 6 of 8 measures for Chile, and 3 out of 5 measures for Argentina.. Each country had at least one measure reflecting transmissibility and at least one reflecting morbidity and mortality for which the correlation was strong or very strong. Chile and Mexico also had at least one measure of disease seriousness for which the correlation was strong.

Conclusions
Our findings suggest that the integration of SARS-CoV-2 into national sentinel surveillance can yield information comparable to that provided by nationwide universal surveillance for measures related to SARS-CoV-2 transmissibility, morbidity and mortality, and seriousness of disease.
Annual estimation of seasonal influenza burden in six South American countries: a retrospective analysis of SARInet surveillance data to inform policies
Authors: Descalzo Miguel Angel , de Paula Júnior Francisco José , Mallegas Natalia Vergara , Penayo Elena , Voto Carla , Goñi Natalia , Bruno Alfredo , Ferreira da Almeida Walquiria Aparecida , do Carmo Greice Madeleine Ikeda , Olivares Barraza María Fernanda , Fasce Rodrigo , Pacheco Jorge , Vázquez Cynthia , Von Horoch Marta , Battaglia Silvia , Giovacchini Carlos , Baumeister Elsa , Santoro Adrián , Buyayisqui María Pía , Alegretti Miguel , Naranjo Mónica Patricia Escobar , Jara Jorge , Nogareda Francisco , Rodríguez Angel , Alvis-Zakzuk Nelson Jose , Iuliano A Danielle , Azziz-Baumgartner Eduardo , Tempia Stefano , Leite Juliana , Rondy Marc , Couto Paula 
Published: February 2025

Abstract

Background
We estimate annual viral influenza-associated mild-to-moderate illness, hospitalizations, and deaths in six South American countries (Argentina, Brazil, Chile, Ecuador, Paraguay, and Uruguay) during the 2015–2019 influenza seasons as a first step in evaluating the full value of influenza vaccination in the subregion.

Methods
We applied a multiplier methodology using monthly hospital discharge and vital statistics death records, influenza surveillance data, and population projections to estimate mild-to-moderate influenza-associated illness, hospitalizations, and deaths. We estimated the uncertainty bounds based on the 2.5th and 97.5th of the Monte Carlo simulated distributions for the number of cases and obtained the ranges from the minimum value of the 2.5th percentile and the maximum value of the 97.5th percentile.

Results
In selected countries with a total population of 307 million people, the yearly influenza-associated burden of disease ranged between 51 and 78 million mild-to-moderate influenza illnesses, between 323,379 and 490,049 hospitalizations, and between 22,662 and 46,971 deaths during the 2015–2019 influenza seasons.

Conclusions
Each year, influenza is associated with millions of illnesses, hundreds of thousands of hospitalizations and tens of thousands of deaths in six South American countries, affecting a significant portion of the population. Such findings can be used to estimate the number of illnesses averted through vaccination programs and the cost-benefit of influenza vaccines.
Influenza vaccine averted illnesses in Chile, Guyana, and Paraguay during 2013–2018: a standardized approach to assess value of vaccination
Authors: Jara Jorge H , Loayza Sergio , Nogareda Francisco , Couto Paula , Descalzo Miguel Angel , Chard Anna N , Olivares Maria , Vergara Natalia , Fasce Rodrigo , Von Horoch Martha , Battaglia Silvia , Penayo Elena , Dominguez Chavely Montserrat , Vazquez Cynthia , Escalada Rainier , Woolford Janice , Michel Fabiana , Chacón Rafael , Fowlkes Ashley , Castro Laura , Velandia-Gonzalez Martha , Rondy Marc , Azziz-Baumgartner Eduardo , Tempia Stefano , Salas Daniel 
Published: February 2025

Abstract

Background
To better establish the value of vaccination against influenza viruses, we estimated vaccine-averted influenza illnesses among young children and older adults in Chile, Guyana, and Paraguay.

Methods
We gathered country- and target population-specific data on monthly influenza hospitalizations, vaccine coverage, and vaccine effectiveness from surveillance records and immunization registries during 2013-2018. We applied a static compartmental model to estimate differences in the number influenza-associated respiratory disease events (symptomatic non-hospitalized illnesses, medically attended illnesses, hospitalizations) in the presence and absence of influenza vaccination programs.

Results
Between 2013 and 2018, vaccinating 68% of children aged 6–23 months in Chile averted an annual mean of 14,617 non-hospitalized, 9,426 medically attended, and 328 hospitalized influenza illnesses; vaccinating 28% of children aged 6–23 months in Paraguay averted 1,115 non-hospitalized, 719 medically attended, and 25 hospitalized influenza illnesses. Vaccinating 59% of older adults in Chile averted an annual mean of 83,429 non-hospitalized, 37,079 medically attended, and 1,390 hospitalized influenza illnesses; vaccinating 36% of older adults in Paraguay averted an annual mean of 3,932 non-hospitalized, 1,748 medically attended, and 66 hospitalized influenza illnesses. In Guyana, a hypothetical campaign vaccinating 30% of children &lt;5 years could have prevented an annual 1,496 non-hospitalized, 971 medically attended, and 10 hospitalized influenza illnesses. Vaccinating 30% of adults ≥65 years could have prevented 568 non-hospitalized, 257 medically attended, and 10 hospitalized influenza illnesses.

Conclusions
Influenza vaccination averted tens of thousands of illnesses and thousands of hospitalizations in Chile and Paraguay; influenza vaccination could have had a proportional benefit in Guyana.
Core cooperative metabolism in low-complexity CO2-fixing anaerobic microbiota
Authors: Zampieri Guido , Santinello Davide , Palù Matteo , Orellana Esteban , Costantini Paola , Favaro Lorenzo , Campanaro Stefano , Treu Laura 
Published: February 2025

Abstract
Biological conversion of carbon dioxide into methane has a crucial role in global carbon cycling and is operated by a specialised set of anaerobic archaea. Although it is known that this conversion is strictly linked with cooperative bacterial activity, such as through syntrophic acetate oxidation, there is also a limited understanding on how this cooperation is regulated and metabolically realised. In this work, we investigate the activity in a microbial community evolved to efficiently convert carbon dioxide into methane and predominantly populated by Methanothermobacter wolfeii. Through multi-omics, biochemical analysis and constraint-based modelling, we identify a potential formate cross-feeding from an uncharacterised Limnochordia species to M. wolfeii, driven by the recently discovered reductive glycine pathway and upregulated when hydrogen and carbon dioxide are limited. The quantitative consistency of this metabolic exchange with experimental data is shown by metagenome-scale metabolic models integrating condition-specific metatranscriptomics, which also indicate a broader three-way interaction involving M. wolfeii, the Limnochordia species, and Sphaerobacter thermophilus. Under limited hydrogen and carbon dioxide, aspartate released by M. wolfeii is fermented by Sphaerobacter thermophilus into acetate, which in turn is convertible into formate by Limnochordia, possibly forming a cooperative loop sustaining hydrogenotrophic methanogenesis. These findings expand our knowledge on the modes of carbon dioxide reduction into methane within natural microbial communities and provide an example of cooperative plasticity surrounding this process.
Microbial reaction rate estimation using proteins and proteomes
Authors: McCain J Scott P , Britten Gregory L , Hackett Sean R , Follows Michael J , Li Gene-Wei 
Published: February 2025

Abstract
Microbes transform their environments using diverse enzymatic reactions. However, it remains challenging to measure microbial reaction rates in natural environments. Despite advances in global quantification of enzyme abundances, the individual relationships between enzyme abundances and their reaction rates have not been systematically examined. Using matched proteomic and reaction rate data from microbial cultures, we show that enzyme abundance is often insufficient to predict its corresponding reaction rate. However, we discovered that global proteomic measurements can be used to make accurate rate predictions of individual reaction rates (median R2 = 0.78). Accurate rate predictions required only a small number of proteins and they did not need explicit prior mechanistic knowledge or environmental context. These results indicate that proteomes are encoders of cellular reaction rates, potentially enabling proteomic measurements in situ to estimate the rates of microbially mediated reactions in natural systems.
Most infants with prenatal Osteogenesis Imperfecta diagnosis and poor prognosis survive: experience of a quaternary care OI Center
Authors: Carroll Ricki S , Little Sarah , McGreal Tina , Bonner Shannon , Willis Daria , Franzone Jeanne M , Campbell Jeffery , Chou Margaret , Raymond Megan B , Schelhaas Andrea , Costa Joanna , Jain Mahim 
Published: February 2025

Abstract
Osteogenesis imperfecta (OI) is a genetic condition with improperly or inadequately produced Type I collagen. Manifestations include bowing deformities, fractures, hydrocephalus, respiratory insufficiency and feeding difficulty. Moderate or severe OI is often diagnosed prenatally based on ultrasound findings and genetic testing may be labeled as lethal. Here we present 18 infants with moderate to severely presenting OI who received neonatal care at a single center over a 5 year period, 10 of which were delivered at our institution. All 18 infants survived to neonatal discharge, with seven infants requiring respiratory support and nine infants requiring feeding support at discharge. Through Fisher Exact Test, Mann Whitney U Test and backward elimination regression, we do not observe that lethal or possibly lethal diagnoses prenatally were correlated with medically relevant outcomes such as need for respiratory support at discharge or need for feeding support at discharge. 16/18 individuals are alive, with a minority requiring either respiratory or feeding support. With a multidisciplinary team approach to neonatal care, outcomes may be optimized. Infants formerly diagnosed with lethal OI may survive. Given our findings, and lack of correlation of prenatal assessments with survival and other medical outcomes, we recommend all families be given the option to pursue medical interventions.
The European earwig Forficula auricularia (Dermaptera: Forficulidae) in California citrus: a sampling method, population surveys, and description of earwig movement into the tree canopy
Authors: Rosenheim Jay A , Cluff Emma , Morrow Kelley 
Published: February 2025

Abstract
The European earwig F. auricularia L. is an omnivore that has only recently been identified as a direct, fruit-feeding pest of citrus. Here, we start to build the basic tools needed for integrated pest management for this species. We introduce a time-efficient sampling method based on small wooden boards placed on the ground, and we use them in a 2-yr survey of 93 commercial citrus blocks in California’s San Joaquin Valley. Insecticides were not applied targeting F. auricularia in any of these citrus blocks. We find that F. auricularia populations are very low or undetectable in most blocks, with higher densities occurring only sporadically. To know when control measures should be implemented, we used video-monitoring of citrus tree trunks to characterize the timing of F. auricularia movement from their soil nests into the tree canopy. Movement of earwigs along the tree trunks was observed throughout our sampling period (22 March to 18 June), suggesting that control measures (sticky bands placed on trunks, or insecticides applied to trunks and surrounding soil surface) should be applied early, well before petal fall when fruit are susceptible to F. auricularia herbivory. Sticky barriers effectively reduced the vertical movement of 2 crawling arthropods, F. auricularia and the Fuller rose beetle Napactus godmanni, along citrus trunks. We failed to find any relationship between estimated F. auricularia densities and damage to maturing or harvested fruit. This highlights a set of important and still unresolved questions about the biology of this species, underscoring the need for additional research.
Rethinking the Informal Economy and the Hugo Effect
Authors: Pappadá Francesco , Rogoff Kenneth S 
Published: February 2025

Abstract
This paper offers a new approach to measuring the size of the informal economy based on VAT data for the European Union. Although data intensive, our EVADE measure is simpler and more transparent than existing measures. EVADE also shows more variation across countries of Europe than earlier measures, including higher informality in Greece, Italy and Spain, for example. Moreover, we find considerably higher variation within countries across time; in a cross-country time series regression, controlling for tax rates, we confirm that the informal economy grows significantly in recessions and decreases in booms, which we term the “Hugo effect”.
Wilhelm Bode und die deutsche Holzskulptur des Spätmittelalters
Authors: Smalcerz Joanna 
Published: February 2025

Abstract
Wilhelm Bode gilt als ein wichtiger, wenn nicht sogar der wichtigste Akteur, der zur Entstehung des Interesses an spätmittelalterlicher deutscher Holzskulptur und darauffolgend sowohl zum Aufbau und zur Entwicklung von Privat- und Museumssammlungen der deutschen Skulptur des Spätmittelalters als auch zur Entwicklung des Kunstmarktes für diese im Kaiserreich, vor allem in Berlin, beigetragen hat. Bodes Geschichte der deutschen Plastik (1887) und die von ihm ausgelöste Konkurrenz zwischen den wichtigsten Berliner Sammlern hat wesentlich zur im Laufe des 19. Jahrhunderts stets steigenden Bedeutung der deutschen spätgotischen Holzskulptur auf dem Kunstmarkt und zum wesentlichen Anstieg ihrer Preise im ersten Jahrzehnt des 20. Jahrhunderts beigetragen. Ziel dieses Aufsatzes ist es, Bodes Rolle in diesem Prozess – als Kunsthistoriker, Museumsmann und Kunstberater – zu untersuchen. Dabei wird die für Bode charakteristische Verflechtung von wissenschaftlicher Tätigkeit und der Beteiligung am Kunstmarkt aufgezeigt.
First clinical experiences with the tetravalent live vaccine against dengue (Qdenga ®) in travellers: a multicentric TravelMedVac study in Germany
Authors: Köpke Clara , Rothe Camilla , Zeder Andreas , Boecken Gerhard , Feldt Torsten , Janke Christian , Jordan Sabine , Köhler Carsten , Löbermann Micha , Müller Andreas , Orth Hans Martin , Prüfer-Krämer Luise Marie , Schäfer Johannes , Slesak Günther , Stich August , Bélard Sabine , Thul Nico , Becker Sören L , Schneitler Sophie ,   TravVacNet-Group
Published: February 2025

Abstract

Background
A study was conducted to assess the safety and tolerability of the tetravalent live-attenuated dengue vaccine Qdenga®, which received marketing approval in Germany in 2022. The study evaluated vaccine-related reactions in a predominantly dengue-naïve population, highlighting the importance of post-marketing surveillance as an essential component of safety evaluation for newly licensed vaccines.

Methods
Following dengue vaccination, participants were recruited for an anonymous online questionnaire through the national ‘Trav VacNet’ network in Germany. The questionnaire focused on post-vaccination reactions up to 18 days after the first and second vaccination, as well as previous travel history and coadministration.

Results
The study included 1176 participants, with a median age of 39 years (IQR 28–56), 53.2% female (n = 625), 46.5% male (n = 547), and 0.3% non-binary participants (n = 4). After the first dose, 51% of the participants reported systemic reactions such as headache (40% (190/474)), weakness (40% (189/474)) and malaise (32% (154/474)), which were most pronounced between days 7–11 post vaccination. After the second dose, localized signs and symptoms such as pain at the injection site (22% (n = 55/250)) were more common. Fever was more common after the first dose (20% (96/474)) vs. 2% (6/250) after the second. Females reported significantly more reactions than males after both vaccinations (1st dose p = 0.0002; 2nd dose p = 0.0003). A total of 334 (28%) co-administrations were reported whereby AEs were reported in 47% (157/333) of participants, with the highest prevalence observed when combined with the Japanese encephalitis vaccine (56.8%, (42/74)). Differences in age groups were observed, with a decrease in reactions in the elderly (≥65 years).

Conclusions
Vaccine-related reactions were frequently reported, predominantly after the first dose in dengue-naïve participants. Coadministration was a common strategy without significantly increasing side effects. The study provides important insights into reactogenicity and may help improve vaccination strategies in dengue-naïve populations.