bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low MYCT1 expression is an independent prognostic predictor and MYCT1 upregulation can induce oral squamous cell carcinoma development
Authors: Xie Yuanyuan , He Jing , An Wenmin , Li Zhenning 
Published: November 2023

Abstract
Background: MYCT1 is an important candidate marker in several kinds of cancer, but its role in oral squamous cell carcinoma (OSCC) remains unknown. In the study, we aimed to explore the biological role and prognostic value of MYCT1 in OSCC.
Methods: MYCT1 expression was analyzed by using the data from The Cancer Genome Atlas (TCGA) in OSCC. MYCT1 expression in 20 pairs of fresh OSCC tissues and adjacent normal tissues was detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting respectively. The correlation of MYCT1 protein level in 106 OSCC tissue samples assayed by immunohistochemical staining to overall survival (OS) and disease-free survival (DFS) of the patients was analyzed using the Kaplan-Meier and Cox proportional hazards methods. The effects of MYCT1 on proliferation, migration, invasion, cell cycle and apoptosis of OSCC cells were monitored by cell viability, colony formation, wound healing, transwell invasion and flow cytometry assays, respectively. The prospective MYCT1-related pathways in OSCC were also predicted by bioinformatic analysis.
Results: TCGA data analysis, qRT-PCR, western blot and Immunohistochemistry results showed that MYCT1 expression was significantly decreased in OSCC tissues compared to the controls. Low MYCT1 expression was associated with large size (P = 0.01), advanced pathological grade (P < 0.001), cervical node metastasis (P=0.005) and worse clinical stage (P <0.001) of the tumor tissues, respectively. The Kaplan-Meier and Cox proportional hazards analyses results indicated that low MYCT1 expression was significantly associated with poor overall survival and recurrence-free survival of the patients. Meanwhile, low MYCT1 expression as an independent prognostic factor for the long-term survival of the OSCC patients was also revealed. Contrasted to MYCT1 silence, MYCT1 overexpression significantly repressed the proliferation, migration and invasion, and promoted apoptosis of the OSCC cells. Bioinformatic prediction results demonstrated that MYCT1 might be involved in ECM−receptor interaction, Focal adhesion and PI3K-Akt signaling pathways of the malignant OSCC progress.
Conclusions: MYCT1 plays a suppressor role in OSCC and serves as a prognostic biomarker for the patients of OSCC, which provides a pivotal clue for MYCT1 in diagnosis and therapy of OSCC.
Optochemical profiling of receptor-proximal proteins in vivo in minutes
Authors: Hamachi Itaru , Takato Mikiko , Sakamoto Seiji , Nonaka Hiroshi , Tamura Tomonori 
Published: November 2023

Abstract

Understanding how protein-protein interaction networks in the brain give rise to cognitive functions necessitates their characterization in live animals. However, tools available for this purpose require potentially disruptive genetic modifications and lack the temporal resolution necessary to track rapid changes in vivo. Here, we combined ligand-directed chemistry and photocatalyzed singlet oxygen generation to identify neurotransmitter receptor-proximal proteins in the live mouse brain using only small-molecule reagents and minutes of photoirradiation. Named PhoxID (photooxidation-driven proximity labeling for proteome identification), our method not only recapitulated the known interactomes of two endogenous neurotransmitter receptors (AMPAR and GABAAR) but also uncovered age-dependent shifts, identifying NECTIN3 and IGSF3 as developmentally regulated AMPAR-proximal proteins in the cerebellum. Overall, this work establishes a flexible and generalizable platform to study receptor microenvironments in genetically intact specimens with an unprecedented temporal resolution.
Code-switching in mental health recovery narratives of Hong Kong storytellers
Authors: Ng Stephanie , Zayts-Spence Olga 
Published: November 2023

Abstract
Background
Considering persistently high levels of mental health stigma in Hong Kong in recent years, scholars and mental health professionals alike have demonstrated strong interest in combating this issue. Existing research suggests that recovery storytelling, which involves an individual with a lived experience of mental illness sharing their personal story publicly, is one of the most effective anti-stigma strategies to date.
Methods
Using a narrative inquiry approach, we examined the recovery stories of seven Cantonese-speaking individuals who participated in the More than a Label mental health anti-stigma campaign in Hong Kong. We specifically examined how the discursive strategy of code-switching functioned to clarify, emphasize, or distract from certain elements of the story.
Results
Our analysis demonstrated that code-switching in our data functioned in three distinct ways, namely as: (1) a distancing strategy when discussing troubling past experiences; (2) an authenticating strategy when recalling interactions carried out in English-speaking settings; and (3) a foregrounding strategy to highlighting the main points in the story.
Conclusions
Our study highlights the importance of linguistic research in informing the development of person-centred approaches in both mental health clinical and advocacy work. We suggest that recognizing linguistic behaviours such as code-switching allows mental health clinical and organizational staff to be more sensitive to signs of potential distress and probe further inquiry amongst clients and lived-experience storytellers. We discuss how these findings can be used to educate lived-experience storytellers on the rhetorical functions of language use such as code-switching.
Topological transformation from non-van der Waals solids to 2D transition metal chalcogenides in molten salts with solvated metals
Authors: Li Mian , Gao Lin , Liang Kun , Huang Qing 
Published: November 2023

Abstract

Transition metal chalcogenides (TMCs) have attracted great attention due to their wide range of applications. The TMCs have been widely synthesized using techniques such as exfoliation and vapor-phase growth, while their structure and constituent tuning remains a challenge. Here, we show a general route to synthesize 2D TMCs by topological transformation of AMX2-type ternary metal chalcogenides in molten salts with solvated metals. In this process, the solvated metals in the molten salts play a role of etchant that can remove the A-layer atoms from the AMX2 structure, resulting in the formation of laminated MX2 layers. The laminated MX2 layers provide natural template for cations intercalation or atom substitution to form 2D TMCs with various structures. In the present work, 2D TMCs with tunable stoichiometries (e.g. Cr2S3, Cr3S4, Cr5S6, and Cr7S8), as well as 2D TMCs with heterostructures (e.g. Cr2S3-Ti0.5CrS2, Cr2S3-NbS2, and Cr2S3-HfS2) were obtained. We further explored the potential of this route to tail the dielectric properties and improve the electromagnetic wave absorption ability of the chromium sulfides. This approach shows advantage in exploring 2D structures with unprecedented constituent and we believe it will open up opportunities for tuning the properties and broaden the functional applications of TMCs.
A simple strategy for synthesis of side-chain sulfonated poly(arylene ether ketone sulfone) constructing hydrophilic/ hydrophobic phase separation structure
Authors: Lei Jinxuan , Xu Jingmei , Meng Lingxin , Zhao Pengyun , Wang Jiayin , Lan Tian 
Published: November 2023

Abstract
A series of poly(aryl ether ketone sulfone) polymers (C-PAEKS-DBS) were synthesized from 4-carboxyphenyl hydroquinone, allyl bisphenol S, and 4,4-difluorobenzophenone by nucleophilic polycondensation. And further a series of side chain sulfonated poly(aryl ether ketone sulfone) proton exchange membranes (CPDA-x) were prepared by simply introducing hydrophilic long alkyl side chains (AMPS) into the polymers by means of double bond cross-linking. The structure of the prepared CPDA-x were characterized by FT-IR, 1H NMR and SEM. This series of composite membranes showed excellent dimensional stability which including moderate water absorption (ranging from 27.91–33.33% at 80oC) and low swelling ratio (ranging from 10.26–16.67% at 80oC). In addition, the prepared films also exhibited high oxidative stability and mechanical property. This simple strategy for the producing of proton exchange membranes (PEMs) has certain potential and may be applied to the producing of fuel cell membranes with hydrophilic and hydrophobic phase separation structures.
Genome editing of RICE FLOWERING LOCUS T 1 promoter delayed flowering in rice
Authors: Wenyan Zhou , Mingliang He , Xiaojie Tian , Qingjie Guan , Xinglong Yu , Qingyun Bu , Xiufeng Li 
Published: November 2023

Abstract
The heading date of rice (Oryza sativa L.) is one of the crucial agronomic traits that affect regional adaptation and grain productivity. Increasing the vegetative growth period or delaying flowering can improve the yield and quality of rice. Therefore, this article uses the CRISPR/Cas9 genome editing system to slightly reduce the expression of flowering activating factors to delay rice flowering. We selected a 0.5-kb region of RICE FLOWERING LOCUS T 1 promoter (RFT1 pro). We designed four pairs of targets in this region using CRISPR-Cas9 for multiplex editing. We obtained six homozygous mutant lines named rft1 pro-1 to rft1 pro-6 by editing the RFT1 pro. The heading date of the mutant lines was delayed by 1.4–9.2 days, and the expression levels of Hd3a and RFT1 were significantly reduced, supporting the late flowering phenotype. Moreover, we found that the protein level of RFT1 was reduced in rft1 pro compared with the wild type, which indicated that editing RFT1 pro affects the translation of RFT1. In conclusion, we have found that it is a feasible method to delay rice flowering by editing the flower-promoting gene promoter. Subsequently, the flowering time of varieties can be fine-tuned by targeting the promoter regions of other genes related to flowering time.
Emamectin·Chlorfenapyr-Induced Fatal Leukoencephalomyelopathy with Delayed Hyperthermia
Authors: Li Xun , yang yun , zhang yajing , Zhang xuebin , zhao na , yue wei 
Published: November 2023

Abstract
Background
Emamectin·chlorfenapyr is compounded with chlorfenapyr and emamectin benzoate. It has been wildly used in agriculture. Although chlorfenapyr toxicity has been verified in animals, the true mechanism and progression in human beings still needs to be unveiled. Furthermore, cases of the compound emamectin·chlorfenapyr poisoning are very scarce.
Case presentation:
We present the case of a 65-year-old female who had attempted suicide by taking 30g of 9.5% chlorfenapyr and 0.5% emamectin benzoate orally 14 days before being admitted to our hospital. Laboratory tests showed extremely high creatinine kinase levels on admission. Magnetic resonance imaging showed diffuse and symmetric T2 hyperintensity in entire white matter tract of brain and spinal cord. Cerebrospinal fluid cytology pathological smear showed abnormal lymphocyte aggregation. 19.5 hours after admission, the patient died because of cardiopulmonary arrest and hyperthermia.
Conclusions
Further research is needed on performing flow cytometry in emamectin·chlorfenapyr intoxication patients and elucidating immunological mechanism beneath the inflammatory process caused by emamectin·chlorfenapyr, and providing new thoughts in antidotal drug development.
Copper homeostasis based on Cuproptosis-related signature optimizes molecular subtyping and treatment of glioma
Authors: Zhang Siyu , Yu Huihan , Sun Suling , Fan Xiaoqing , Bi Wenxu , Li Shuyang , Wang Wei , Fang Zhiyou , Chen Xueran 
Published: November 2023

Abstract
Copper is essential in living organisms and crucial to various physiological processes. Normal physiological conditions are in a state of copper homeostasis to ensure normal biochemical and metabolic processes. Dysregulation of copper homeostasis has been associated with multiple diseases, especially cancer. Cuproptosis is a copper-dependent cell death mediated by excess copper or homeostasis dysregulation. Elesclomol is a common inducer of cuproptosis, carrying copper into the cell and producing excess copper. Cuproptosis modulates tumor proliferation-related signaling pathways and is closely associated with remodeling the tumor microenvironment. In gliomas, the role of cuproptosis and copper homeostasis needs to be better characterized. This study systematically analyzed cuproptosis-related genes (CRGs) and constructed a cuproptosis signature for gliomas. The signature closely links the subtypes and clinical features of glioma patients. The results showed a greater tendency toward dysregulation of copper homeostasis as the malignant grade of glioma patients increased. In addition, CRGs-signature effectively predicted the sensitivity of glioma cells to elesclomol and verified that elesclomol inhibited glioma mainly through inducing cellular cuproptosis. In summary, we found different copper homeostatic features in gliomas and verified the anticancer mechanism of elesclomol, which provides a theoretical basis for developing novel therapeutic strategies for gliomas.
Public support for carbon pricing policies and different revenue recycling options: a systematic review and meta-analysis of the survey literature
Authors: Valencia Farah Mohammadzadeh , Mohren Cornelia , Ramakrishnan Anjali , Merchert Marlene , Minx Jan , Steckel Jan 
Published: November 2023

Abstract

Identifying policy conditions that foster public support is critical for implementing effective direct and indirect carbon pricing policies. We conduct a systematic review and meta-analysis to examine the survey-based literature on change in public support for carbon pricing policies when different revenue recycling options are presented to respondents. Following a comprehensive and transparent machine-learning assisted screening of the literature, our dataset comprises 35 studies containing 70 surveys across 26 countries covering a total of more than 100,000 respondents. We find that the introduction of revenue recycling options increases public support for carbon pricing scheme, but to different degrees depending on recycling scheme and region. Our meta-regression results indicate that green spending (i.e. using revenues for climate-friendly projects) is the only revenue recycling option associated with a statistically significant increase in public support for carbon pricing policies when compared to an allocation of the revenues towards public finance (i.e. using revenues government budget purposes). Our findings moreover suggest that the effect of revenue recycling options on public support depends on the region, and is statistically stronger in Africa, Asia and Pacific, and Latin America and Caribbean compared to North America.
Human-altered soil loss contributes to nearly half of water erosion in China
Authors: Wang Zhen , li Keke , Yang Jingya , Wang Jingyu , Zeng Yi , Borrelli Pasquale , Hubacek Klaus , Hu Yuanchao , Xu Baodong , Fang Nufang , Zeng Chen , Zhou Zhanhang , Shi Zhihua 
Published: November 2023

Abstract
Although the cause-and-effect relationship between accelerated soil erosion and human activities has been confirmed by geological records, the magnitude and socio-economic drivers of the anthropogenic contribution remain largely unexplained at the country scale. Here, we present an integrated modelling framework that uses multi-source high spatial resolution datasets to assess human-altered soil erosion (HASE) by water and its drivers. We estimate China’s territorial HASE to be equal to 1.35 billion tonnes yr-1 (reference year 2017). This is the result of 2.74 billion tonnes yr-1 of HASE triggered by land-use change activities denoted as HASEluc (~1.75 times the estimated natural soil erosion, denoted as SEnat), and 1.39 billion tonnes yr-1 alleviated by soil conservation measures. Modelling activities further indicate that the mitigation due to terraces may have the largest effect on HASE (0.94 billion tonnes yr-1). Concerning socio-economic activities, such as consumption behaviours, we observe that they can indirectly perturb earth surface processes and have a relatively large HASE footprint. Household consumption, especially the final demand from Northeast China and associated industrial activities along the entire supply chain could be the major underlying factors driving HASE. Our findings highlight the intervention of soil erosion by humans and call for comprehensive soil erosion control measures from a social-ecological coupled perspective.
Estimation of Prevalence and Predictors of Sub-clinical Peripheral Neuropathy in Indian adolescents with Type-1 Diabetes: a Case-Control study
Authors: Mondkar Shruti Arvind , Desai Keyur , Oza Chirantap , Borse Sandeep , Yewale Sushil , Dange Nimisha , Ganacharya Raj , Khadilkar Vaman , Khadilkar Anuradha V 
Published: November 2023

Abstract
Aims: Comparison of nerve conduction (NCS) parameters of adolescents with type-1 Diabetes (T1D) with controls. Assessment of sub-clinical peripheral neuropathy prevalence and predictors in Indian adolescents with T1D.
Methods: Case-control study. Cases(n=120):10-19 years, T1D duration>2 years. Exclusion: Comorbidities, illnesses affecting nerve function. Controls(n=40):Healthy, age, sex-matched.
Results: Mean age:15.1±3 years; diabetes duration:7±3.5 years, HbA1c:9.6±2.1% (81mmol/mol). None had symptoms/signs of peripheral neuropathy. Both groups were similar in anthropometry, physical activity. NCS comparison demonstrated significantly prolonged latency, reduced action potential duration and nerve conduction velocity (p<0.01) in motor nerves (median, ulnar, peroneal, tibial) in patients with T1D. Sensory action potential duration in median and ulnar nerves, and sural conduction velocity were significantly lesser in T1D (p<0.05).
70% had at least one abnormal NCS parameter. Sub-clinical neuropathy prevalence- demyelinating motor: upper limbs (UL)-5.8%, lower limbs (LL)-12.5%; demyelinating sensory:UL-52.5%, LL-5.0%; axonal motor:UL-19.2%, LL-10.8%; axonal sensory: UL-11.7%, LL-6.7%. Eight (6.6%) had stage-1a distal symmetrical polyneuropathy. Poor glycemic control (OR=2.0), reduced insulin sensitivity (OR=1.8), advancing age (OR=1.4), reduced iron intake (OR=0.8) were significant predictors of sub-clinical neuropathy.
Conclusion: The prevalence of sub-clinical peripheral neuropathy in Indian adolescents with T1D was high, with the highest prevalence of sub-clinical neuropathy being noted in the upper limbs. Despite no symptoms, NCS parameters were adversely affected in T1D, highlighting the need for early NCS-based screening. Poor glycemic control, reduced insulin sensitivity, advancing age, reduced iron intake were predictors of sub-clinical neuropathy.
Effects of neuromuscular electrical stimulation on quadriceps muscle strength,exercise capacity,quality of life and dyspnea in severe COPD: A systematic review and Meta-analysis of Randomized Controlled Trials
Authors: Lu Zhi , Dong Qi-Yu , Wu Chuan-Fang , Shu Ling , Liang Bing , Yang Hong-Zhong , Tan Chuang 
Published: November 2023

Abstract

Background This review is to determine the impact of NMES on quadriceps muscle strength, exercise capacity, health-related quality of life, and dyspnea in patients with severe COPD.
Methods From the beginning until June 2023, data sources such as EMBASE, PubMed, Web of Science, PEDro database, and Cochrane database were analyzed.Randomized controlled trials were conducted to evaluate the effects of NMES alone versus usual care or NMES combined exercise versus exercise in patients with severe COPD. Outcomes included strength of the quadriceps, exercise capacity, and health-related quality of life. Language or publication dates were not mandatory. These studies were selected independently by two authors.
Results There were 600 participants in fifteen studies, all of which met the inclusion criteria. Compared with usual care, NMES improved quadriceps muscle strength (SMD = 0.93, 95% CI: 0.37 to 1.48; p = 0.003) and exercise capacity (SMD = 1.17, 95% CI: 0.41 to 1.93; p = 0.003) and alleviated breathing difficulties (SMD = − 0.64, 95% CI: −0.89 to − 0.40; p < 0.00001). There were no statistically significant differences between the VO2 peak and the SGRQ. Compared with exercise alone, NMES combined with exercise not improved quadriceps muscle strength (SMD = 0.17, 95% CI: −0.62 to 0.96; p = 0.67), exercise capacity (SMD = 0.11, 95% CI: −0.18 to 0.39; P = 0.47), dyspnea (SMD = − 0.21, 95% CI: −0.51 to 0.09; P = 0.17), and VO2 peak value. However, statistically significant differences were observed in the SGRQ (SMD = − 0.48, 95% CI:−0.84 to 0.22; P = 0.009). No adverse events were reported.
Conclusions
In patients with severe COPD, NMES can improve their quadriceps muscle strength, exercise capacity, and dyspnea. NMES is not beneficial when compared to exercise alone. Therefore, the added cost of incorporating NMES into rehabilitation programs should be considered.
PROSPERO registration number CRD42023433703.
Interventions to reduce sedentary behavior in adults with cardiovascular disease: A scoping review
Authors: Peng Zhihang , Yang Lili , Deng Shunfeng 
Published: November 2023

Abstract

Background Individuals with cardiovascular disease spend much time being sedentary, while sedentary behavior is associated with increased cardiovascular risk as an independent, modifiable factor. However, there is no synthesis of evidence on sedentary behavior interventions for adult cardiac patients. This scoping review aimed to identify interventions to reduce sedentary behavior in adults with cardiovascular disease. Methods Arksey and O'malley's scoping review methodological framework guided the implementation of the review. Seven databases were searched from inception to January 23, 2022 with manually searched reference lists. We included 11 eligible studies involving interventions for sedentary behavior in adults with cardiovascular disease. Data were extracted regarding intervention characteristics, types, delivery formats, and outcome measurements. Results Multiple intervention types and delivery modes were applied, but few were theory-based. Of all the studies, seven interventions were delivered through mobile or internet technologies. Self-monitoring of behavior is the most common behavior change technique used in interventions. Most studies significantly reduced sedentary behavior after the interventions, but the long-term follow-up effects remain uncertain. Conclusions Sedentary behavior in adults with cardiovascular disease holds promise for reduction after interventions, but its long-term adherence needs to be explored. Additionally, future interventions are recommended to use multiple behavior change techniques to reduce sedentary behavior and clarify specific patterns of sedentary behavior reduction.