bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the yellow fever outbreak in Angola from December 2015 through December 2016: A retrospective study
Authors: Manuel Eusebio , Armando António , Francisco Moisés , Paixão Joana , Aramburu Javier , de Oliveira Miguel dos Santos , Freitas Helga , Pedro Alda Morais , Jandondo Domingos , Carderon Pablo Babrero , Lamezon Sandra Lopez , Fortes Filomeno , Mariscal Jorge , Cardoso Yolanda , Moreira Rosa , Morais Joana , Francisco Ngiambudulu M. 
Published: February 2024

AbstractBackground and AimsThe acute tropical infectious disease known as yellow fever (YF) is caused by an arbovirus and is characterized by fever, jaundice, hemorrhage, headache, muscle pain, nausea, vomiting, and fatigue. Angola experienced a yellow fever virus (YFV) outbreak that was documented in December 2015. However, little is known about the outcome of this outbreak. We aimed to demonstrate epidemic features and lessons learned during the YF epidemic in Angola.MethodsA total of 4618 blood samples from suspected YF cases were sent to the Instituto Nacional de Investigação em Saúde (INIS), a national referral and public health laboratory, between December 5, 2015, and December 23, 2016. Sample analyses were conducted using enzyme‐linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT‐PCR) assays. Blood samples were sent from 16 out of the 18 provinces of Angola.ResultsWe detected 884 (19.1%) cases that were positive for ELISA, which were confirmed by RT‐PCR assay. Considering the positive cases, the incidence among male patients was around three times higher (n = 223; 10.9%) than in female patients (n = 59; 2.6%) in the 20–29 age group, followed by the age group 10–19 with n = 211 (6.8%) in males versus n = 108 (3.3%) in females; and the age group 30–39 had n = 68 (4.8%) in males versus n = 28 (1.8%) in females. The other groups had an incidence below 3.0%. The case fatality ratio for YF was in young adults in the age group 20–29 with n = 39 cases, followed by the age group 10–19 with n = 16 cases, and finally the age group 0–9 with n = 13 cases. The other age groups had several deaths by YF below 10 cases.ConclusionsThis study demonstrates features of the YF epidemic that occurred in Angola. Also, it demonstrates that YF causes deaths in young people but is preventable by high vaccine coverage. Thus, public health laboratory surveillance must be strengthened to reduce the possibility of emerging and re‐emerging human infections.
NR5A2 promotes malignancy progression and mediates the effect of cisplatin in cutaneous squamous cell carcinoma
Authors: Ye Wang , Ya‐xuan Cao , Shan‐shan Tang , Qiu Long , Ting Ma , Shao‐jie Chen , Yu Cao 
Published: February 2024

AbstractIntroductionNuclear receptor subfamily five group A member two (NR5A2) plays a key role in the development of many tumor types, while it is uncertain in cutaneous squamous cell carcinoma (cSCC). The aim of this work was to determine the role of NR5A2 in cSCC proliferation, and to determine whether NR5A2 mediates the effect of cisplatin in cSCC.MethodsWe performed a systematic study of existing data and conducted a preliminary bioinformatics analysis of NR5A2 expression in cSCC using bioinformatics databases. Immunohistochemical staining was performed on cSCC tissues of seven patients to study NR5A2 expression. NR5A2 expression was examined in human keratin‐forming cells (HaCaT) and human cSCC cells (A431, Colo‐16, SCL‐1, SCL‐2, and HSC‐5). Stable A431 and SCL‐2 cell lines consisting of sh‐RNA‐NR5A2 were constructed to detect changes in cell proliferation, cell cycle, apoptosis, and to determine the key proteins in the Wnt/β‐catenin pathway. We also investigated changes in the effects of cisplatin on cSCC cells by CCK‐8, clone formation assay, and Flow apoptosis assay after NR5A2 knockdown.ResultsNR5A2 showed enhanced expression in cSCC tissues than in healthy tissues. Downregulation of NR5A2 in cSCC cells led to the formation of a less malignant phenotype. In contrast, the proliferative capacity of the cSCC cells was enhanced posttreatment with RJW100, an NR5A2 agonist. Additionally, NR5A2 knockdown led to a decrease in the expression level of the proteins in the Wnt/β‐catenin pathway, and this inhibition was reversed by LiCl and recombinant antibody, Wnt3a. Moreover, NR5A2 knockdown resulted in diminished proliferative capacity and increased apoptotic cells after the addition of cisplatin.ConclusionNR5A2 plays a crucial role in the progression of cSCC, and the Wnt/β‐catenin signaling pathway may be involved in the regulation of NR5A2‐mediated cSCC. Knockdown of NR5A2 enhanced both the proliferation inhibiting and apoptosis promoting effects of cisplatin on cSCC.
Structure‐Activity Relationships of 2‐(Arylthio)benzoic Acid FTO Inhibitors
Authors: Yan Chao , Zhang Qian , Xiao Pan , Xie Xinyun , Li Ming , Qiu Yuanlai , Wen Liufa , Song Xiaomin , Dong Ze , Yang Cai‐Guang 
Published: February 2024

AbstractThe biological role of the fat mass and obesity‐associated protein (FTO) in the initiation and progress of acute myeloid leukemia (AML) has been elucidated, and several representative FTO inhibitors can markedly suppress the proliferation of AML cells. We previously developed FTO inhibitors including FB23. In this study, we adopted bioisosteric replacement of the intramolecular hydrogen bond in FB23 with a sulfur‐oxygen interaction to generate a series of 2‐(arylthio)benzoic acid FTO inhibitors and established their structure‐activity relationships. Compound 8c was the most potent 2‐(arylthio)benzoic acid FTO inhibitor with an IC50 value of 0.3±0.1 μM, which was comparable with that of FB23 in vitro. To enhance the antiproliferative effects in AML cell lines, we applied a prodrug strategy and prepared some esters. 7l, the methyl ester of 8l, exerted a superior inhibitory effect on a panel of AML cancer cell lines. Additionally, 7l treatment notably increased global m6A abundance in AML cells. Collectively, our data suggest that 2‐(arylthio)benzoic acid may be a new lead compound for inhibition of FTO, and the prodrug analog exhibit potential in the treatment of AML.
Myomectomy with ArtiSential fully articulating laparoscopic instruments: A prospective multicenter study
Authors: Won Seyeon , Kim Kidong , Lee Keun Ho , Kim Tae‐Joong , Kim Taehun , Hwang Jong Ha , Park Hyun , Kim Min Kyu , Hong Dae Gy , Song Yong Jung , Seong Seok Ju 
Published: February 2024

AbstractBackgroundArtiSential, a class of innovative laparoscopic instrument, has been developed to overcome the limitations of conventional laparoscopic surgery by enabling free, 360°‐unrestricted movement of the wrist joint, as in robotic surgery.ObjectiveThe aim of the present study was to describe the initial experiences with these devices in myomectomy and to report the surgical outcomes.MethodsA total of 77 women undergoing laparoscopic or robotic myomectomy between January 2021 and June 2022 were included in this multicenter prospective study. The ArtiSential instruments used by the surgeons were those chosen according to their respective preferences. The baseline characteristics, surgical outcomes, trocar placement options, and operator survey results were scrutinized.ResultsThe mean age of the patients was 39.9 ± 6.3, and the mean body mass index (BMI, calculated as weight in kilograms divided by the square of height in meters) was 22.4 ± 3.4 kg/m2; 46.8% of the patients underwent robotic surgery, while 53.2% underwent laparoscopic surgery. The number of removed myomas was 3.3 ± 3.0, the size of the largest myoma was 7.1 ± 2.3 cm, and the operative time was 130.0 ± 54.0 min. No transfusions or laparotomy conversions were required. Other than one case of ileus, there were no postoperative complications. In most cases, the instruments were inserted through the umbilicus trocar, and the fenestrated forceps, needle holder, and bipolar fenestrated forceps, in that order, were frequently employed. According to a surgeon survey, 29.9% moderately or strongly agreed that the ArtiSential devices utilized were more convenient than conventional laparoscopic instruments, while only 9.7% moderately or strongly agreed that they were more convenient than robotic instruments.ConclusionsMyomectomy as performed with an ArtiSential instrument seems to be feasible and safe. Further studies are necessary in order to comparatively assess the outcomes and potential benefits of ArtiSential, robotic, and conventional laparoscopic myomectomy.
Effect of rechallenge nivolumab in a hemodialysis patient with multiple metastases from a rapidly progressed T1a renal clear cell carcinoma: An autopsy case
Authors: Hanawa Kazushi , Sawada Norifumi , Yokota Yuka , Aikawa Junki , Otake Yuko , Sugimura Koki , Shimura Hiroshi , Mochizuki Takanori , Kira Satoru , Mitsui Takahiko 
Published: February 2024

IntroductionDistant metastasis of T1a renal cell carcinoma is rare and whether metastasis is more probable in patients undergoing hemodialysis remains unclear. We report the autopsy case of a patient undergoing hemodialysis with multiple metastases that rapidly progressed from T1a renal cell carcinoma treated with multimodal therapy including nivolumab.Case presentationA 70‐year‐old male who underwent hemodialysis was diagnosed with clear cell carcinoma (pT1a, G2) after nephrectomy. Six months post‐surgery, bone and lung metastases appeared and treated with radiotherapy and pazopanib, respectively. Nivolumab was administered as second‐ and fourth‐line treatments for lung metastases. The patient died approximately 60 months after initial diagnosis; however, nivolumab controlled disease progression for 24 months. An autopsy revealed the lung's occupation with clear cell carcinoma tumor tissue.ConclusionNivolumab has potential to control lung metastasis progression. Additionally, rechallenge is possible in patients with renal cell carcinoma undergoing hemodialysis.
Toward a further understanding of assent
Authors: Morris Cody , Oliveira Jacob P. , Perrin Jesse , Federico Caitlyn A. , Martasian Paula J. 
Published: February 2024

AbstractArranging assent opportunities is an increasingly common strategy for involving clients in therapeutic decisions within behavior analysis. Recent behavior‐analytic articles have helped create a basic behavioral definition and conceptualization of assent, but much more guidance is needed for practitioners and researchers interested in embedding assent into their practices. The purpose of this article is to advance the conceptualization and understanding of assent and assent practices by refining previous definitions and conceptualizations of assent and providing six essential considerations for embedding assent into practice. The six considerations consist of determining the applicability and feasibility of assent, assessing assent‐related skills, arranging assent procedures and teaching assent‐related skills, arranging fair choices, selecting opportunities to assess assent, and informally assessing assent. Following the discussion of the considerations for assent practices, we issue a call for specific topics of research on assent.
Factors associated with attaining a pharmacy residency interview: A scoping review
Authors: Whitten Suzanne , Phillips Beth Bryles , Osae Sharmon P. , Stone Rebecca H. , Keedy Chelsea A. , Johnson Blake R. , Prentiss Amber , Lavender Devin L. 
Published: February 2024

AbstractPostgraduate pharmacy residencies are essential in preparing graduates for pharmacy careers. Residencies are highly competitive and despite efforts to increase the number of residency programs in the United States, the demand still outweighs the supply. Attaining a residency interview is a critical step in securing a residency position. The objective of this scoping review is to describe the published literature on factors associated with attaining a pharmacy residency interview. The updated Arksey and O'Malley scoping review framework was followed for this scoping review. The review is reported according to The Preferred Reporting Items for Systematic Reviews and Meta‐Analyses Extension for Protocols and Scoping Reviews (PRISMA‐ScR). The search strategy contained a combination of medical subject headings (MeSH) and keywords from a review of article title and abstracts. The inclusion and exclusion criteria were created a priori. English language studies describing pharmacy residency interview, selection, rubrics, and descriptive, quantitative, or qualitative data were included. Title and abstracts were independently reviewed, followed by independent full‐text reviews. Covidence©, an online systematic review platform, was used in the screening, review, and selection process. Thirteen studies assessing factors associated with a residency interview met inclusion criteria. The majority of these studies (n = 7) analyzed data from residency program director (RPD) surveys. Most of these studies found that RPDs highly value letters of recommendation when evaluating a candidate's application. Three studies analyzed retrospective data. Several studies focused on letters of recommendation. The studies described letters of recommendation, letters of intent, grade point average, and leadership experience as factors associated with attaining a residency interview. Most studies had limitations in generalizability, study design, and heterogeneous data.
XBRL reporting in firms with data breach incidents
Authors: Jiang Wanying , Xu Chunhao , Counts Roy Wayne 
Published: February 2024

AbstractThe Securities and Exchange Commission (SEC) adopted new rules mandating that firms disclose cybersecurity incidents and risk management procedures for inline XBRL reporting, highlighting the regulator's concern about firms’ response to data breaches. In this study, we examine whether firms use XBRL strategically to hinder external stakeholders from understanding the impact of announced data breaches. We find that firm XBRL filing complexity increases following data breaches. Further investigation suggests that the increased XBRL complexity is concentrated on financial statement note tags instead of financial statement tags. The findings imply that firms with data breach incidents are likely to increase XBRL reporting complexity to mitigate stock market reactions. We also find that analysts following moderate the relationship between the data breach and XBRL reporting timeliness. These findings provide empirical evidence about XBRL reporting changes after data breach incidents and contribute to cybersecurity literature and XBRL filing regulation.
Humoral immune response and safety of SARS‐CoV‐2 vaccination in very early onset inflammatory bowel disease
Authors: Kastl Arthur J. , Weaver Kimberly N. , Zhang Xian , Strople Jennifer A. , Adler Jeremy , Kelsen Judith R. , Dubinsky Marla C. , Bousvaros Athos , Watkins Runa , Dai Colin , Cross Raymond K. , Higgins Peter D. R. , Ungaro Ryan , Bewtra Meenakshi , Bellaguarda Emanuelle A. , Farraye Francis A. , Chun Kelly , Zikry Michael , Bastidas Monique , Boccieri Margie E. , Firestine Ann , Long Millie D. , Kappelman Michael D. 
Published: February 2024

AbstractChildren with very early onset inflammatory bowel disease (VEO‐IBD) may respond differently to coronavirus disease 2019 (COVID‐19) immunization compared to healthy children or other patients with IBD. We recruited children with VEO‐IBD <6 years of age and younger following receipt of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) vaccine. Demographics, IBD characteristics, medication use, adverse events (AEs) and IBD exacerbations were collected. Blood draws (optional) were obtained for measurement of antireceptor binding domain (RBD) IgG antibodies following vaccination. Of 41 participants, none required emergency department visit or hospitalization due to AE, and only one experienced IBD exacerbation. Detectable antibody was present in 19/19 participants who provided blood sample; 6/7 participants (86%) had durable humoral response 12 months postvaccination. Children with VEO‐IBD experience robust humoral immune response to COVID‐19 immunization. Severe AEs were rare. These findings provide reassurance that children with VEO‐IBD respond well and safely to SARS‐CoV‐2 vaccination.
Effect of maturation at birth on the clinical features of neonatal cow's milk protein allergy: A retrospective study
Authors: Nagafuji Motomichi , Fujiyama Satoshi , Ishii Ryota , Shime Michiru , Kitatsu Toshinori , Hoshino Yusuke , Kanai Yu , Arai Junichi , Miyazono Yayoi , Takada Hidetoshi 
Published: February 2024

AbstractNeonatal immune regulation transitions from fetal immunity and varies with maturation status, but its role in neonatal cow's milk protein allergy (CMPA) remains unknown. We studied the association between maturation status at birth and neonatal CMPA. Clinical and laboratory data of neonates presenting with CMPA symptoms were retrospectively collected from two tertiary hospitals. Patients were assessed according to gestational age at birth: preterm, late‐preterm, and full‐term. Fifty‐five infants (26 females, 14 preterm, 15 late‐preterm, and 26 full‐term) were included; 44 were negative for milk‐specific immunoglobulin E. Neonatal CMPA was common during moderately premature periods. Preterm infants exhibited longer latency from initial CM exposure to disease onset, lower incidence of bloody stool, and absence of elevated monocyte counts. However, immunoreactivity to CM antigens was retained in all infants. Neonatal CMPA features varied with infant maturation status at birth. Our results improve the understanding of intestinal immunity development, fetal/neonatal immune regulation, and CMPA pathogenesis.
Development of a rapid detection assay for acetolactate synthase inhibitors resistance in three Amaranthus weed species through loop‐mediated isothermal amplification
Authors: Milani Andrea , Panozzo Silvia , Grazia Tommasini Maria , Scarabel Laura 
Published: February 2024

AbstractBACKGROUNDThe early detection of herbicide resistance in weeds is a key factor to avoid herbicide waste and improve agriculture sustainability. The present study aimed to develop and validate an allele‐specific loop‐mediated isothermal amplification (AS‐LAMP) assay for the quick on‐site detection of the resistance‐endowing point mutation Trp‐574‐Leu in the acetolactate synthase (ALS) gene in three widely diffused Amaranthus weed species: Amaranthus retroflexus, Amaranthus hybridus and Amaranthus tuberculatus.RESULTSThe AS‐LAMP protocol was developed on wild‐type and ALS‐mutant plants of the three species and revealed that the amplification approach with only the primer set specific for the mutant allele (574‐Leu) was the most promising. The validation and estimation of the AS‐LAMP performance evaluated by comparing the results with those of the molecular marker (cleaved amplified polymorphic sequences) indicated that, although the sensitivity and specificity were relatively high in all species (overall 100 and > 65%, respectively), precision was high for A. hybridus L. and A. retroflexus L. (75 and 79%, respectively), but quite low for A. tuberculatus (Moq.) J. D. Sauer (59%). The LAMP assay was also effective on crude genomic DNA extraction, allowing the quick detection of mutant plants in field situation (on site resistance detection).CONCLUSIONThe proposed AS‐LAMP method has proven to be a promising technique for rapid detection of resistance as a result of Trp‐574‐Leu on the two monoecious weedy Amaranthus species but resulted less effective in the genetically variable dioecious species A. tuberculatus. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography
Authors: Wang Zhenqiang , Yu Jinqian , Zhao Lei , Niu Tong , Wang Xiao 
Published: February 2024

In the present study, twelve compounds from Dioscorea spongiosa were successfully purified by an efficient technique combined bioassay‐guided fractionation macroporous resin column chromatography (MRCC) pretreatment and high‐speed counter‐current chromatography (HSCCC) separation for the first time. Then, D101 MRCC was used to fractionate the crude extract into five parts, which further applied the bioassay‐guided fractionation strategy to screen the active fractions of 2 and 4. As for the separation, 200 mg Fr.2 was purified by HSCCC using EtOAc/n‐BuOH/H2O (2:2:3, v/v), leading to annulatomarin (1), dioscoresides C (2), diosniponol C (3), methyl protodioscin (4), pseudoprotodioscin (5), protogracillin (6), as well as 200 mg Fr.4 yielding montroumarin (7), dioscorone A (8), diosniponol D (9), protodioscin (10), gracillin (11), and dioscin (12) using CH2Cl2/MeOH/H2O (3:3:2, v/v) with the purities over 95.0%. Finally, the isolates were assayed for their anti‐inflammatory, urico‐lowering, and anti‐diabetic activities in vitro, which indicated that the steroidal saponins of 5, 6, and 11 showed all these three activities.
Novel Approach to Color‐Neutral Transparent Solar Cells: An Organic‐Si Hybrid Achieved by Laser Microhole Drilling
Authors: Ho Ngoc Thanh , Lee Seongyu , Joo Beom Soo , Kim Jagyeong , Kang JoonHyun , Kim Jin Hong , Kang Gumin , Ko Hyungduk 
Published: February 2024

AbstractTransparent solar cells (TSCs) hold promise for applications in building‐integrated photovoltaics (BIPVs) and vehicles. However, existing TSCs often have compromised color neutrality and efficiency because of the distinctive colors arising from their photoactive materials. The study proposes a novel fabrication method for TSCs based on Si wafers with the average visible transmittance (AVT) controlled by creating a microhole pattern through a combination of nanosecond laser drilling and subsequent wet etching. Through this ambient‐air process, organic–Si hybrid TSCs are successfully realized by integrating an organic PEDOT:PSS layer onto this structure with a neutral color. The power conversion efficiency (PCE) is adjusted by the fraction of the perforated region, and it attained 6.20% at 30% AVT, 5.37% at 40% AVT, and 4.67% at 50% AVT. In this hybrid TSC approach, the CIE color coordinates are closely aligned with the neutral‐color points (0, 0), resulting in color rendering index (CRI) values exceeding 99. The strategy represents a significant step toward the development of efficient color‐neutral TSCs for transparent solar applications.