bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphisms in the Vitamin D Receptor Gene Are Associated With Periodontal Disease
Authors: de Brito Junior Rui Barbosa , Scarel‐Caminaga Raquel Mantuaneli , Trevilatto Paula Cristina , Souza Ana Paula , Barros Silvana Pereira 
Published: February 2005

Background: Genetic polymorphisms in the vitamin D receptor (VDR) gene are associated with bone homeostasis and diseases in which bone loss is a cardinal sign. The aim of this study was to determine whether chronic periodontal disease in a Brazilian population is associated with polymorphisms in the VDR gene.Methods: Clinical examination and recordings of probing depth and clinical attachment level were performed in 113 unrelated adults who were divided into two groups: 44 healthy individuals (control group) and 69 subjects with chronic periodontitis (CP). DNA was obtained from the subjects' epithelial cells by scraping the buccal mucosa. Two polymorphisms in the VDR gene were analyzed by polymerase chain reaction, followed by TaqI and BsmI restriction endonuclease digestion.Results: Frequencies of VDR/TaqI and VDR/BsmI showed significant differences between the control group and the CP group (P <0.05). The “Tb” haplotype was prevalent in the control group (43.2%), and the “TB” haplotype in the CP group (36.6%). The “TB” haplotype seemed to increase susceptibility to periodontal disease (odds ratio [OR] = 2.19). The heterozygous haplotype “TB/tb” was predominant in the CP group (OR = 4.32; P = 0.005).Conclusions:TaqI and BsmI polymorphisms of the VDR gene are associated with clinical attachment loss due to periodontal disease in a Brazilian population. These findings suggest that VDR genotype might be a risk indicator for susceptibility to chronic periodontitis. J Periodontol 2004;75:1090‐1095.
Osseointegration in a Sinus Augmented With Bovine Porous Bone Mineral: Histological Results in an Implant Retrieved 4 Years After Insertion. A Case Report
Authors: Scarano Antonio , Pecora Gabriele , Piattelli Maurizio , Piattelli Adriano 
Published: February 2005

Background: A subject of controversy in implant dentistry is what is the most appropriate material for sinus augmentation. Little is known about the healing pattern and the osseointegration processes at the interface of implants placed in different grafting materials in man. Bovine porous bone mineral (BPBM) is a xenogenic material similar to human cancellous bone with a high biocompatibility and osteoconductivity.Methods: A 50‐year‐old patient presented a posterior left maxilla with an insufficient bone height for implant insertion. In January 1999, a sinus lifting procedure was performed and the left sinus was augmented with BPBM; in November 1999, three implants were inserted, and in July 2000 a fixed prosthetic restoration was positioned. In June 2003 the connecting screw of the middle implant fractured and it was decided to remove the implant using a 5 mm trephine bur.Results: All BPBM particles were surrounded by newly formed bone. No BPBM particle was in direct contact with the implant surface, and between the implant surface and the particles there was newlyformed bone. BPBM seemed to undergo a very slow resorption; the particles constituted 36% ± 3% of the peri‐implant tissues. The bone‐implant contact percentage was 72% ± 4%.Conclusions: In our specimens, we found an intimate, direct contact between bone and implant without an interposition of the graft material particles. A very high bone‐to‐implant contact percentage was present. Our results show that the slow resorption of the graft particles did not jeopardize the osseointegration of the implant. J Periodontol 2004;75:1161‐1166.
A Randomized Clinical Trial Comparing Enamel Matrix Derivative and Membrane Treatment of Buccal Class II Furcation Involvement in Mandibular Molars. Part II: Secondary Outcomes
Authors: Meyle Jörg , Gonzales José R. , Bödeker Rolf H. , Hoffmann Thomas , Richter Steffen , Heinz Bernd , Arjomand Mehrdad , Reich Elmar , Sculean Anton , Jepsen Karin , Jepsen Søren 
Published: February 2005

Background: This multicenter, randomized trial compared enamel matrix derivative (EMD) with barrier membranes for the treatment of Class II mandibular furcations with regard to secondary outcomes. The influence of furcation morphology on the effectiveness of either treatment was also evaluated.Methods: Forty‐eight patients (age range 28 to 73 years; 22 females, 26 males) with buccal Class II furcation involvements in both contralateral lower first or second molars were included. After initial periodontal treatment, defects were randomized to either EMD or bioabsorbable guided tissue regeneration (GTR) barrier. Study design and the results for the primary parameter were previously described. Results of the following secondary outcome variables are reported here: changes of the hard tissue boundaries describing the anatomical situation of the furcation defect and changes in the following clinical parameters between baseline and 14 months: plaque, level of gingival margin, probing depth, bleeding on probing, attachment level, and bone sounding at five sites/tooth at the buccal side. Descriptive statistics were applied for changes in clinical parameters and measurements of hard tissue boundaries. The differences observed under treatment with EMD or membrane were analyzed by means of the Wilcoxon two‐sample test. The difference between the effect of the EMD and membrane treatment was estimated by means of the Hodges‐Lehmann estimator.Results: Overall, similar healing results were observed for both treatments. However, there was slightly more recession in the mid‐furcation site following membrane treatment (P = 0.04). Additionally, different treatment effects could be detected for the distances from the stent or cemento‐enamel junction (CEJ) to the buccal bone crest, mid‐distal root (P stent = 0.01; PCEJ = 0.07) and for the distance from the stent or CEJ to the buccal bone crest, mid‐mesial root (P stent = 0.01; PCEJ = 0.01). There was no measurable bone resorption in EMD sites, whereas a slight resorption occurred with membrane treatment. Furcation morphology at the time of surgery was not associated with clinical outcome, irrespective of the treatment.Conclusion: With regard to secondary outcome parameters, enamel matrix derivative treatment led to a similar regenerative result as the membrane procedure. J Periodontol 2004;75:1188‐1195.
Relative Proportions of T‐Cell Subpopulations and Cytokines That Mediate and Regulate the Adaptive Immune Response in Patients With Aggressive Periodontitis
Authors: Suárez Lina J. , Ocampo Ana M. , Dueñas Ricardo E. , Rodríguez Adriana 
Published: February 2005

Background: Both the virulence factors of periodontopathic bacteria and the immune response against them have been involved in tissue destruction observed in periodontal disease. Considering the regulatory role of cytokines produced by T cells, the purpose of this study was to compare the CD3+, CD4+, and CD8+ subpopulations of T cells, and to characterize the mRNA of cytokines involved in the adaptive immune response in a group of healthy/gingivitis 1 (HI/G1) individuals and aggressive periodontitis (AgP) patients.Methods: The percentages of T‐cell subpopulations were analyzed in 10 gingival samples of HI/G1 individuals and 10 gingival samples of AgP patients by immunohistochemistry. The presence of interleukin (IL)‐2, interferon (IFN)‐γ, IL‐4, IL‐5, IL‐10, IL‐13, and transforming growth factor (TGF)‐β was measured by reverse transcription polymerase chain reaction (RT‐PCR) of mRNA extracted from complete gingival biopsies.Results: Significant differences were found in CD3+ and CD4+ cell counts between both groups. The parameters were lower in the gingival biopsies from AgP patients while CD8+ counts were similar in both groups. The cytokine mRNA analysis showed constant expression of IL‐2 and IFN‐γ in all cases. The mRNA of IL‐5 and IL‐10 was present in the majority of HI/G1 (N = 10, N = 9, respectively) but was not in the AgP group (N = 2, N = 1). IL‐13 and TGF‐β were only detected in HI/G1 (N = 2, N = 3) and IL‐4 was not detected in any of the individuals.Conclusions: These results indicate that the role of the CD8+ subpopulation in aggressive periodontitis lesions is limited. On the other hand, cytokines IL‐2 and IFN‐γ may not be relevant in the progression of aggressive periodontitis. J Periodontol 2004;75: 1209‐1215.
Locally Applied Isosorbide Decreases Bone Resorption in Experimental Periodontitis in Rats
Authors: Leitão R.F.C. , Rocha F.A.C. , Chaves H.V. , Lima V. , Cunha F.Q. , Ribeiro R.A. , Brito G.A.C. 
Published: February 2005

Background: The role of nitric oxide (NO) on bone metabolism is controversial, since it can either stimulate bone formation or resorption. We investigated the effect of local administration of the NO donor isosorbide in an experimental periodontal disease model.Methods: Wistar rats were subjected to a ligature placement around the cervix of the right second upper molar and were sacrificed after 11 days. Alveolar bone loss was measured in one quadrant as the sum of the distances between the cuspid tip and the alveolar bone along the axis of each molar root, which was subtracted from the contralateral side, used as unligated control. The semiquantitative histopathological scale of the periodontium was based on cell infiltration and alveolar bone and cementum integrity. Groups were treated with a gel containing 1% or 5% isosorbide applied to the vestibular side of the molar gingiva 1 hour before the placement of the ligature and then twice daily until sacrifice. Controls included one group subjected to periodontitis and no treatment (NT) and another that received the gel containing just the vehicle (V).Results: The application of the vehicle gel produced an increase of the alveolar bone resorption, without altering the inflammatory changes, compared to the NT group. The 5% isosorbide produced a significant reduction of the alveolar bone resorption, compared to V and NT. This reduction was confirmed by histological analysis, showing less inflammatory cell infiltration and preservation of the cementum and the alveolar process.Conclusion: Local application of isosorbide reduces alveolar bone resorption in experimental periodontal disease in rats, suggesting a local anti‐inflammatory effect of isosorbide. J Periodontol 2004;75:1227‐1232.
Effects of Implant Thread Geometry on Percentage of Osseointegration and Resistance to Reverse Torque in the Tibia of Rabbits
Authors: Steigenga Jennifer , Al‐Shammari Khalaf , Misch Carl , Nociti Francisco H. , Wang Hom‐Lay 
Published: February 2005

Background: Dental implant thread geometry has been proposed as a potential factor affecting implant stability and the percentage of osseointegration. Therefore, the aim of this prospective, randomized, parallel arm study was to evaluate the effects of dental implant thread design on the quality and percent of osseointegration and resistance to reverse torque in the tibia of rabbits.Methods: Seventy‐two custom‐made, screw‐shaped, commercially pure titanium implants (3.25 mm diameter × 7 mm length) were placed in the tibiae of 12 white New Zealand rabbits. Each tibia received three implants of varying thread shapes: one with a Vshaped, one with a reverse buttress, and one with a square thread design. The rabbits were sacrificed following an uneventful healing period of 12 weeks. Implants in the right tibiae underwent histologic and histomorphometric assessments of the bone‐to‐implant contact (BIC) and the radiographic density of surrounding bone, while implants in the left tibiae were used for reverse‐torque testing. Differences between the three thread designs were examined using analysis of variance (ANOVA).Results: Data showed that the square thread design implants had significantly more BIC and greater reverse‐torque measurements compared to the V‐shaped and reverse buttress thread designs, while no differences were found in radiographic bone density assessments.Conclusion: These results indicate that the square thread design may be more effective for use in endosseous dental implant systems. J Periodontol 2004;75:1233‐1241.
Further Evidence of the Association Between Periodontal Conditions and Coronary Artery Disease
Authors: Geerts Sabine O. , Legrand Victor , Charpentier Joseph , Albert Adelin , Rompen Eric H. 
Published: February 2005

Background: There is increasing evidence that chronic infections, such as periodontal diseases, could play a role in the initiation and development of coronary artery disease (CAD). The present study was intended to test for a possible association between presence and severity of periodontitis and coronary artery disease in a Belgian population.Methods: A total of 108 CAD patients (mean age 59.2 ± 11 years) and 62 presumably healthy controls (mean age 57.7 ± 9 years) were enrolled in the study. Probing depth, periodontal pocket bleeding index (PPBI), plaque index, furcation involvements, and tooth mobility were evaluated to compare periodontal health in both groups. The subjects were also ranked according to a novel index of periodontitis severity, the periodontal index for risk of infectiousness (PIRI), aimed at quantifying the risk of release of proinflammatory mediators from the periodontal sites.Results: Periodontitis was significantly more frequent in CAD patients than in controls (CAD patients: 91%; controls: 66%). The mean number of pockets was 18 ± 17.1 in cardiac patients versus 7.6 ± 12.7 in controls (P <0.0001), despite the fact that the mean number of missing teeth was significantly greater in cases than in controls (14 ± 7.1 versus 9 ± 5.2; P <0.0001). Furthermore, proportions of mobile teeth, bleeding sites, periodontal pockets, and involved furcations were significantly higher in CAD patients than in controls. In addition, the extent of the periodontal disease present was also greater in cases than in controls. A logistic model, adjusted for known cardiovascular risk factors, showed a strong association between CAD and periodontitis (odds ratio [OR] = 6.5). Moreover, there was a significant dose‐response relationship between increasing scores of the periodontal risk of infectiousness and the presence of CAD (adjusted OR = 1.3 per PIRI unit).Conclusion: In the present study, periodontitis was revealed to be a significant risk factor for CAD after adjusting for other confounding factors, with the level of association increasing with the individual extent of the periodontal lesions. J Periodontol 2004;75:1274‐1280.
Enhancement of Periodontal Tissue Regeneration by Transplantation of Bone Marrow Mesenchymal Stem Cells
Authors: Kawaguchi Hiroyuki , Hirachi Akio , Hasegawa Naohiko , Iwata Tomoyuki , Hamaguchi Hidenori , Shiba Hideki , Takata Takashi , Kato Yukio , Kurihara Hidemi 
Published: February 2005

Background: The use of suitable cells transplanted into periodontal osseous defects appears to be a powerful strategy to promote periodontal tissue regeneration. Mesenchymal stem cells (MSCs) isolated from bone marrow have the potential for multilineage differentiation. The purpose of this study was to examine whether auto‐transplantation of MSCs into periodontal osseous defects would be useful for periodontal tissue regeneration.Methods: Bone marrow MSCs were isolated from beagle dogs and expanded in vitro. The expanded MSCs were mixed with atelocollagen (2% type I collagen) at final concentrations of 2 × 106, 5 × 106, 1 × 107, or 2 × 107 cells/ml, and auto‐transplanted into experimental Class III defects. Atelocollagen alone was implanted into the defects as a control. Periodontal tissue healing was evaluated by histological and morphometric analyses 1 month after transplantation.Results: The defects were regenerated with cementum, periodontal ligament, and alveolar bone in the MSC‐atelocollagen groups. Less periodontal tissue regeneration was observed in the control group compared to the MSC‐atelocollagen groups. Morphometric analysis revealed that the percentage of new cementum length in the 5 × 106 and 2 × 107 cells/ml groups and the percentage of new bone area in the 2 × 107 cells/ml group were significantly higher than in the control group (P <0.01).Conclusion: These findings suggest that auto‐transplantation of bone marrow mesenchymal stem cells is a novel option for periodontal tissue regeneration. J Periodontol 2004;75:1281‐1287.
Thermal stability, structural features, and B‐to‐Z transition in DNA tetraloop hairpins as determined by optical spectroscopy in d(CG)3T4(CG)3 and d(CG)3A4(CG)3 oligodeoxynucleotides
Authors: Hernández Belén , Baumruk Vladimir , Gouyette Catherine , Ghomi Mahmoud 
Published: February 2005

AbstractNMR and CD data have previously shown the formation of the T4 tetraloop hairpin in aqueous solutions, as well as the possibility of the B‐to‐Z transition in its stem in high salt concentration conditions. It has been shown that the stem B‐to‐Z transition in T4 hairpins leads to S (south)‐ to N (north)‐type conformational changes in the loop sugars, as well as anti to syn orientations in the loop bases. In this article, we have compared by means of UV absorption, CD, Raman, and Fourier transform infrared (FTIR), the thermodynamic and structural properties of the T4 and A4 tetraloop hairpins formed in 5′‐d(CGCGCG‐TTTT‐CGCGCG)‐3′ and 5′‐d(CGCGCG‐AAAA‐CGCGCG)‐3′, respectively. In presence of 5M NaClO4, a complete B‐to‐Z transition of the stems is first proved by CD spectra. UV melting profiles are consistent with a higher thermal stability of the T4 hairpin compared to the A4 hairpin. Order‐to‐disorder transition of both hairpins has also been analyzed by means of Raman spectra recorded as a function of temperature. A clear Z‐to‐B transition of the stem has been confirmed in the T4 hairpin, and not in the A4 hairpin. With a right‐handed stem, Raman and FTIR spectra have confirmed the C2′‐endo/anti conformation for all the T4 loop nucleosides. With a left‐handed stem, a part of the T4 loop sugars adopt a N‐type (C3′‐endo) conformation, and the C3′‐endo/syn conformation seems to be the preferred one for the dA residues involved in the A4 tetraloop. © 2005 Wiley Periodicals, Inc. Biopolymers 78: 21–34, 2005
Kefir yeast technology: Scale‐up in SCP production using milk whey
Authors: Koutinas A.A. , Athanasiadis I. , Bekatorou A. , Iconomopoulou M. , Blekas G. 
Published: February 2005

AbstractA three‐step process to scale‐up kefir biomass production at a semi‐industrial scale employing whey is reported. Aerobic fermentations were initially performed at laboratory scales, in 1.5‐ and 4‐L bioreactors, yielding 79 g/L final kefir biomass (0.89 g/g of lactose utilized), in 7 h of fermentation time. The use of whey as carbon source even in solid cultures led to the formation of a granular biomass. These results encouraged scale‐up at a semi‐industrial‐scale pilot plant employing 100‐ and 3,000‐L bioreactors, leading to the development of a process for granular kefir biomass production. The results validated the laboratory‐scale experiments and the avoidance of centrifugal separators due to granular biomass formation. Pilot‐plant operations showed kefir to be highly resistant to contamination under actual industrial conditions and no serious problems in handling of raw materials and equipment were observed. Economic analysis showed a 20% higher cost of the market price of products, with added value of up to 15.9 × 109 € within the European Union. © 2005 Wiley Periodicals, Inc.
Preparation and Characterization of Dimercury(I) Monofluorophosphate(V), Hg2PO3F: Crystal Structure, Thermal Behavior, Vibrational Spectra, and Solid‐State 31P and 19F NMR Spectra.
Authors: Weil Matthias , Puchberger Michael , Baran Enrique J. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
[N(CH2CH2NH3)3] [U3O2F13]×2H2O: A Novel Organically Templated Mixed‐Valent Uranium Oxyfluoride with a Hybrid Network Structure.
Authors: Wang Chih‐Min , Liao Chia‐Hsien , Lin Hsiu‐Mei , Lii Kwang‐Hwa 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Mixed Cation Nickel Diphosphate with a Layered Intergrowth Structure: Synthesis, Structural and Magnetic Characterization of Na(NH4) [Ni3(P2O7)2 (H2O)2].
Authors: Liu Wei , Yang Xin Xin , Chen Hao Hong , Huang Ya Xi , Schnelle Walter , Zhao Jing Tai 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
tert‐Amino Effect in Heterocyclic Chemistry. Synthesis of Hydrogenated Spiro Derivatives of Quinolines.
Authors: D'yachenko E. V. , Glukhareva T. V. , Nikolaenko E. F. , Tkachev A. V. , Morzherin Yu. Yu. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactions of Alkyl 2‐Benzylidene‐2‐polyfluoroacylacetates with N,N‐Dinucleophiles.
Authors: Pryadeina M. V. , Burgart Ya. V. , Kodess M. I. , Saloutin V. I. , Chupakhin O. N. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Poly[styrene(iodosodiacetate)]‐Promoted Ring Expansion Reaction of 1‐Alkynylcycloalkanols: A Novel Synthesis of (Z)‐2‐(1‐Iodo‐1‐organyl)methylenecycloalkanones.
Authors: Chen Jiang‐Min , Huang Xian 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Barium Dichromate [BaCr2O7], a Mild Reagent for Oxidation of Alcohols to Their Corresponding Carbonyls in Non‐Aqueous Polar Aprotic Media.
Authors: Mottaghinejad Enayatollah , Shaafi E. , Ghasemzadeh Z. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactions of Diethyl Dibromomalonate and Ethyl 2,2‐Dichloroacetoacetate with Water and Carbonyl Compounds (Aldehydes and Ketones) in the Presence of Pentacarbonyliron.
Authors: Terent'ev A. B. , Vasil'eva T. T. , Mysova N. E. , Chakhovskaya O. V. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
New Access to 1,3‐Diketones from Aldehydes.
Authors: Fargeas Valerie , Baalouch Myriam , Metay Estelle , Baffreau Jerome , Menard Delphine , Gosselin Pascal , Berge Jean‐Pascal , Barthomeuf Chantal , Lebreton Jacques 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Diels—Alder Reaction of Polychlorocyclopentadienes with Vinyl‐ and Allylacetylene Dienophiles.
Authors: Veliev M. G. , Chalabieva A. Z. , Shatirova M. I. , Mamedov I. M. , Mamedov E. Sh. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Chiral Vanadyl Salen Complex Anchored on Supports as Recoverable Catalysts for the Enantioselective Cyanosilylation of Aldehydes. Comparison Among Silica, Single Wall Carbon Nanotube, Activated Carbon and Imidazolium Ion as Support.
Authors: Baleizao Carlos , Gigante Barbara , Garcia Hermenegildo , Corma Avelino 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Highly Enantioselective Strecker Reaction Catalyzed by Titanium‐N‐salicyl‐β‐aminoalcohol Complexes.
Authors: Banphavichit Vorawit , Mansawat Woraluk , Bhanthumnavin Worawan , Vilaivan Tirayut 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Nitrogen‐Functionalized Cyclohexanes Using Chemoselective Conjugate Addition of Phenyllithium to Linear ω‐Nitro‐α,β,ψ,ω‐Unsaturated Ester and Subsequent Stereoselective Intramolecular Nitro‐Michael Cyclization.
Authors: Yasuhara Tomohisa , Nishimura Katsumi , Osafune Emi , Muraoka Osamu , Tomioka Kiyoshi 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Photochromic 2,3‐Bis(2,5‐dimethyl‐3‐thienyl)‐3‐cyanoacrylates by the Beckmann Rearrangement of a Cyclobutenedione Derivative.
Authors: Shirinian Valerii Z. , Krayushkin Mikhail M. , Barachevskii Valerii A. , Belen'kii Leonid I. , Shimkin Aleksei A. , Strokach Yuri P. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Esters of 4,5‐Dihydro‐1H‐imidazole‐1‐oxyl‐3‐oxide‐carboximidic Acid.
Authors: Tretyakov E. V. , Romanenko G. V. , Shvedenkov Yu. G. , Ovcharenko V. I. , Sagdeev R. Z. 
Published: February 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.