bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blockade of TNF-α/TNFR2 signalling suppresses colorectal cancer and enhances the efficacy of anti-PD1 immunotherapy by decreasing CCR8+T regulatory cells
Authors: Guo Yixian , Xie Feng , Liu Xu , Ke Shouyu , Chen Jieqiong , Zhao Yi , Li Ning , Wang Zeyu , Yi Gang , Shen Yanying , Li Dan , Zhu Chunchao , Zhang Zizhen , Zhao Gang , Lu Hong , Li Bin , Zhao Wenyi 
Published: November 2023

Abstract
The enrichment of regulatory T cells (Tregs) in the tumour microenvironment (TME) has been recognized as one of the major factors in the initiation and development of resistance to immune checkpoint inhibitors. C–C motif chemokine receptor 8 (CCR8), a marker of activated suppressive Tregs, has a significant impact on the functions of Tregs in the TME. However, the regulatory mechanism of CCR8 in Tregs remains unclear. Here, we revealed that a high level of TNF-α in the colorectal cancer (CRC) microenvironment upregulated CCR8 expression in Tregs via the TNFR2/NF-κB signalling pathway and the FOXP3 transcription factor. Furthermore, in both anti-programmed cell death protein 1 (anti-PD1)-responsive and anti-PD1-unresponsive tumour models, PD1 blockade induced CCR8+ Treg infiltration. In both models, Tnfr2 depletion or TNFR2 blockade suppressed tumour progression by reducing CCR8+ Treg infiltration and thus augmented the efficacy of anti-PD1 therapy. Finally, we identified that TNFR2+CCR8+ Tregs but not total Tregs were positively correlated with adverse prognosis in patients with CRC and gastric cancer. Our work reveals the regulatory mechanisms of CCR8 in Tregs and identifies TNFR2 as a promising target for immunotherapy.
Effects of transfluthrin-treated jute and cotton clothing against resistant and susceptible Aedes aegypti (Diptera: Culicidae) in a semifield system
Authors: Ahebwa Alex , Hii Jeffrey , Neoh Kok-Boon , Leepasert Theerachart , Chareonviriyaphap Theeraphap 
Published: November 2023

Abstract
Volatile pyrethroids exert a range of both lethal and behavioral effects on mosquitoes through the passive release of insecticides into the atmosphere. We investigated the protective efficacy (PE) of transfluthrin-treated jute (TI-jute) and cotton (TI-cotton) fabrics, worn at the back of a protective black vest, against laboratory-reared pyrethroid susceptible and resistant strains of Aedes aegypti (L.) in a semifield system (SFS). Each fabric (1,029 cm2) was treated with 1.79 mg/cm2 of transfluthrin as the intervention. Human landing collections were conducted by 2 collectors seated in designated treatment and control compartments of the SFS. The trials were conducted for 41 days, with 16 days partitioned into morning and evening phases. Furthermore, we examined blood feeding behavior and fecundity of the surviving mosquitoes post-exposure. Results showed that in the morning, the PE of TI-jute (49.4%) was higher than that of TI-cotton (36.8%). TI-jute demonstrated a lower PE of 9.6% against the transfluthrin-resistant strain. Remarkably, a significantly higher number of eggs were laid by the transfluthrin-resistant mosquitoes that survived the intervention (36.5 eggs/female) compared to the control group (11.8 eggs/female). These findings suggest that TI-jute can help protect against bites and alter the life traits of Ae. aegypti. The study highlights that the timing of the intervention during the day affected the efficacy of TI-jute and TI-cotton, while sublethal exposure to transfluthrin stimulated egg production in the resistant strain. These are critical challenges that warrant attention in vector control strategies. Investigating this phenomenon in mosquito reproduction necessitates future research at a molecular level.
The apple transcription factor MdZF-HD11 regulates fruit softening by promoting Mdβ-GAL18 expression
Authors: Wang Miaomiao , Wu Yao , Zhan Wenduo , Wang Hao , Chen Ming , Li Tongxin , Bai Tuanhui , Jiao Jian , Song Chunhui , Song Shangwei , Feng Jiancan , Zheng Xianbo 
Published: November 2023

Abstract
Fruit ripening and the associated softening are major determinants of fruit quality and post-harvest shelf life. Although the mechanisms underlying fruit softening have been intensively studied, there are limited reports on the regulation of fruit softening in apples (Malus domestica). Here, we identified a zinc finger homeodomain transcription factor MdZF-HD11that trans-activates the promoter of Mdβ-GAL18, which encodes a pectin-degradation enzyme associated with cell wall metabolism. Both MdZF-HD11 and Mdβ-GAL18 genes were up-regulated by exogenous ethylene treatment and repressed by 1-methylcyclopropene treatment. Further experiments revealed that MdZF-HD11 binds directly to the Mdβ-GAL18 promoter and up-regulates its transcription. Moreover, using transgenic apple fruit calli, we found that overexpression of Mdβ-GAL18 or MdZF-HD11 significantly enhanced β-galactosidase activity, and overexpression of MdZF-HD11 induced the expression of Mdβ-GAL18. We also discovered that transient overexpression of Mdβ-GAL18 or MdZF-HD11 in ‘Golden Delicious’ apple significantly increased the release of ethylene, reduced fruit firmness, promoted the transformation of skin color from green to yellow, and accelerated ripening and softening of the fruit. Finally, the overexpression of MdZF-HD11 in tomato also promoted fruit softening. Collectively, these results indicate that ethylene-induced MdZF-HD11 interacts with Mdβ-GAL18 to promote the post-harvest softening of apple.
VR as a metaleptic possible world of global citizenship embodiment: a cognitive stylistic approach
Authors: Fawzy Rania Magdi 
Published: November 2023

Abstract
Bringing together narrative elements, virtual affordances, and participants’ embodied interactions, virtual reality (VR) movies instantiate new narrative techniques by offering an immersive experience. This study examines virtual narrative beyond mere interactional engagement and extends the phenomenon to include worlding, metaleptic embodiment, and instantiated possible selves. It aims at exploring VR narrative as idiosyncratic cognitive processes, with a special focus on the notions of empathy and emotional involvement as significant elements contributing to this peculiar interactional and cognitive experience. A cognitive stylistic approach is adopted to explain the functional ability of VR technology in transporting participants to alternate worlds and in making them experience a kind of self-transformation. The immersively metaleptic discourse of Baba Yaga is examined as engaging participants in a quest of how to act as morally and socially empathetic and responsible citizens—global citizens. Baba Yaga narrative deploys the narrative discourses of flashbacks, facework, doubly deictic ‘you’, performatives, and imperatives along with material processes to situate participants in a virtual space of actions and doings and hence encourage them to configure their desired self(ves) across different immersive interactions. The global citizen is embodied in the interactive narrative of Baba Yaga, through invoking various storyworld possible selves (SPSs): the feeling self, the responsible self, and the moral self, which encompasses climate activist self and interculturally aware self who manages to get rid of its own cultural biases as the narrative proceeds. Embodied in these selves, participants transform the virtual world into possible worlds of their own passion, agency, choices, hopes, and desires.
How does the presence of bar affects the fueling of supermassive black holes? An IllustrisTNG100 perspective
Authors: Kataria Sandeep Kumar , Vivek M 
Published: November 2023

ABSTRACT
We conducted a statistical study of black hole masses of barred and unbarred galaxies in the IllustrisTNG100 cosmological magnetohydrodynamical simulations. This work aims to understand the role of the bars in the growth of central supermassive black hole mass and its implications on AGN fueling. Our sample consists of 1191 barred galaxies and 2738 unbarred galaxies in the IllustrisTNG100 simulations. To have an unbiased study, we perform our analysis with an equal number of barred and unbarred galaxies by using various controlled parameters like total galaxy mass, stellar mass, gas mass, dark matter halo mass, etc. Except for the stellar mass controlling, we find that the median of the black hole mass distribution for barred galaxies is higher than that of the unbarred ones, indicating that stellar mass is a key parameter influencing the black hole growth. The higher mean accretion rate of the black holes in barred galaxies, averaged since the bar forming epoch (z ∼ 2), explains the higher mean black hole masses in barred galaxies. Further, we also test that these results are unaffected by other environmental processes like minor/major merger histories and neighbouring gas density of black hole. Although the relationship between stellar mass, bar formation, and black hole growth is complex, with various mechanisms involved, our analysis suggests that bars can play a crucial role in feeding black holes, particularly in galaxies with massive stellar discs.
Intensity and velocity oscillations in a flaring active region
Authors: Millar David C L , Fletcher Lyndsay , Joshi Jayant 
Published: November 2023

ABSTRACT
Chromospheric oscillations can give us insight into the physical environment in the solar atmosphere, both in quiet Sun and flaring conditions. Many authors have reported increases in the prevalence of 3-minute oscillations which are thought to be excited by events which impact the chromosphere such as flares. In this study, we utilized the Ca ii 8542 Å line to study the oscillatory behaviour of the chromosphere in an active region which underwent two B-class flares. We analysed oscillations in both intensity and velocity, and found different behaviours in both. Intensity oscillations were most prevalent over the umbrae of sunspots and magnetic pores in the active region, and the extent of the area which contained significant oscillations was found to decrease when comparing times after the flares to before. By measuring the evolution of the magnetic field, we found that this could be because the areas surrounding the umbrae were becoming more ‘penumbral’ with an increase to the magnetic field inclination. Velocity oscillations were found across the active region both before and after the flares but were observed clearly in areas which were brightened by the second flare. By comparing to EUV imaging, it was seen that strong chromospheric velocity oscillations with 3–4-minute periods occurred at the same time and location as a flare loop cooling 30 min after the second flare peak. This could be evidence of disturbances in the loop exciting a response from the chromosphere at its acoustic cut-off frequency.
Search for the wide-orbit massive companion of XO-7b in the follow-up radial-velocity and transit-timing data: no significant clues
Authors: Garai Z , Pribulla T , Komžík R 
Published: November 2023

ABSTRACT
XO-7b is a hot Jupiter transiting a V = 10.52 mag G0V-type star. The planetary system is interesting because the linear slope in the discovery radial-velocity (RV) data indicated a wide-orbit massive companion. In 2020 we started an RV campaign for the system with the main scientific goal to follow-up this linear slope, and to put constraints on the orbital period of the companion. Furthermore, we aimed at refining the system parameters and we wanted to probe transit time variations (TTVs) of XO-7b in order to search for long-term dynamical signs of the companion of XO-7b in the observed-minus-calculated (O-C) data of mid-transit times. Apart from the discovery RVs, we obtained and analysed 20 follow-up RV observations and TESS photometric data. The previously observed significant linear RV slope was not confirmed with the follow-up RV data, where we detected only a marginal linear slope with the opposite trend. If the announced companion really exists, the most convincing explanation is that both RV data sets were collected near its quadrature position. Based on the RVs we estimated the minimum orbital period, which is Porb, min, 3 ≳ 7900 ± 1660 d, and the ‘minimum’ minimum mass of the companion, which is (M3sin i)min = 16.7 ± 3.5 MJup. We did not find significant evidence of the companion of XO-7b in the O-C data set of mid-transit times. We can again conclude that if the announced companion really exists, this is in agreement with previous results that distant companions of exoplanets are only known by RV solutions.
Immediate drop of urine osmolality upon tolvaptan initiation predicts impact on renal prognosis in patients with ADPKD
Authors: Akihisa Taro , Kataoka Hiroshi , Makabe Shiho , Manabe Shun , Yoshida Rie , Ushio Yusuke , Sato Masayo , Yajima Aiji , Hanafusa Norio , Tsuchiya Ken , Nitta Kosaku , Hoshino Junichi , Mochizuki Toshio 
Published: November 2023

ABSTRACT

Background
Tolvaptan, a vasopressin V2 receptor antagonist, is used for treating autosomal dominant polycystic kidney disease (ADPKD). We focused on changes in urinary osmolality (U-Osm) after tolvaptan initiation to determine whether they were associated with the therapeutic response to tolvaptan.

Methods
This was a single-centre, prospective, observational cohort study. Seventy-two patients with ADPKD who received tolvaptan were recruited. We analysed the relationship between changes in U-Osm and annual estimated glomerular filtration rate (eGFR) in terms of renal prognostic value using univariable and multivariable linear regression analyses.

Results
The mean value of U-Osm immediately before tolvaptan initiation was 351.8 ± 142.2 mOsm/kg H2O, which decreased to 97.6 ± 23.8 mOsm/kg H2O in the evening. The decrease in U-Osm was maintained in the outpatient clinic 1 month later. However, the 1-month values of U-Osm showed higher variability (160.2 ± 83.8 mOsm/kg H2O) than did those in the first evening of tolvaptan administration. Multivariate analysis revealed that the baseline eGFR, baseline urinary protein and U-Osm change in the evening of the day of admission (initial U-Osm drop) were significantly correlated with the subsequent annual change in eGFR.

Conclusions
U-Osm can be measured easily and rapidly, and U-Osm change within a short time after tolvaptan initiation may be a useful index for the renal prognosis in actual clinical practice.
Longitudinal drug synergy assessment using convolutional neural network image-decoding of glioblastoma single-spheroid cultures
Authors: Giczewska Anna , Pastuszak Krzysztof , Houweling Megan , Abdul Kulsoom U , Faaij Noa , Wedekind Laurine , Noske David , Wurdinger Thomas , Supernat Anna , Westerman Bart A 
Published: November 2023

Abstract

Background
In recent years, drug combinations have become increasingly popular to improve therapeutic outcomes in various diseases, including difficult to cure cancers such as the brain cancer glioblastoma. Assessing the interaction between drugs over time is critical for predicting drug combination effectiveness and minimizing the risk of therapy resistance. However, as viability readouts of drug combination experiments are commonly performed as an endpoint where cells are lysed, longitudinal drug-interaction monitoring is currently only possible through combined endpoint assays.

Methods
We provide a method for massive parallel monitoring of drug interactions for 16 drug combinations in 3 glioblastoma models over a time frame of 18 days. In our assay, viabilities of single neurospheres are to be estimated based on image information taken at different time points. Neurosphere images taken on the final day (day 18) were matched to the respective viability measured by CellTiter-Glo 3D on the same day. This allowed to use of machine learning to decode image information to viability values on day 18 as well as for the earlier time points (on days 8, 11, and 15).

Results
Our study shows that neurosphere images allow us to predict cell viability from extrapolated viabilities. This enables to assess of the drug interactions in a time window of 18 days. Our results show a clear and persistent synergistic interaction for several drug combinations over time.

Conclusions
Our method facilitates longitudinal drug-interaction assessment, providing new insights into the temporal-dynamic effects of drug combinations in 3D neurospheres which can help to identify more effective therapies against glioblastoma.
Optoelectronic tuning of plasmon resonances via optically modulated hot electrons
Authors: Yao Jiacheng , Wang Cheng , Zhang Chi , Ma Song , Zhou Li , Wang Ti , Wang Ququan , Xu Hongxing , Ding Tao 
Published: November 2023

ABSTRACT
Fast optical modulation of nanoplasmonics is fundamental for on-chip integration of all-optical devices. Although various strategies have been proposed for dynamic modulation of surface plasmons, critical issues of device compatibility and extremely low efficiency in the visible spectrum hamper the application of optoplasmonic nanochips. Here we establish an optoplasmonic system based on Au@Cu2–xS hybrid core–shell nanoparticles. The optical excitation of hot electrons and their charge transfer to the semiconductor coating (Cu2–xS) lead to lowered electron density of Au, which results in the red shift of the localized surface plasmon resonance. The hot electrons can also transport through the Cu2–xS layer to the metal substrate, which increases the conductance of the nanogap. As such, the coupled gap plasmon blue-shifts with a magnitude of up to ∼15 nm, depending on the excitation power and the thickness of the coatings, which agrees with numerical simulations. All of this optoelectronic tuning process is highly reversible, controllable and fast with a modulated laser beam, which is highly compatible and sufficiently useful for on-chip integration of nanophotonic devices.
Rapid growth in a large Cambrian apex predator
Authors: Wu Yu , Pates Stephen , Pauly Daniel , Zhang Xingliang , Fu Dongjing 
Published: November 2023

ABSTRACT
Despite the importance of ontogenetic data on early diverging euarthropods to our understanding of the ecology and evolution of past life, the data are distinctly lacking, as reconstructing life histories of fossil animals is often challenging. Here we report the growth trajectory of frontal appendages of the apex predator Amplectobelua symbrachiata, one of the most common radiodont arthropods from the early Cambrian Chengjiang biota (c. 520 Ma) of China. Analysis of 432 specimens (9.1–137.1 mm length; 1.3–25.6 mm height) reveals that appendages grew isometrically, with an estimated maximum size of the whole animal of c. 90 cm. Individuals grew rapidly compared to extant arthropods, as assessed using the electronic length-frequency analysis (ELEFAN) approach. Therefore, we show that the Cambrian apex predator A. symbrachiata was an extremely fast-growing arthropod, with an unusual life history strategy that formed as part of the escalatory ‘arms race’ that shaped the Cambrian explosion over 500 Ma.
Introducing Quin: The Design and Development of a Prototype Chatbot to Support Smoking Cessation
Authors: Bendotti Hollie , Ireland David , Lawler Sheleigh , Oates David , Gartner Coral , Marshall Henry M 
Published: November 2023

AbstractIntroductionChatbots emulate human-like interactions and may usefully provide on-demand access to tailored smoking cessation support. We have developed a prototype smartphone application-based smoking cessation chatbot, named Quin, grounded in real-world, evidence-, and theory-based smoking cessation counseling sessions.MethodsConversation topics and interactions in Quitline counseling sessions (N = 30; 18 h) were characterized using thematic, content, and proponent analyses of transcripts. Quin was created by programming this content using a chatbot framework which interacts with users via speech to text. Reiterative changes and additions were made to the conversation structure and dialogue following regular consultation with a multidisciplinary team from relevant fields, and from evidence-based resources.ResultsChatbot conversations were encoded into initial and scheduled follow-up “appointments.” Collection of demographic information, and smoking and quit history, informed tailored discussion about pharmacotherapy preferences, behavioral strategies, and social and professional support to form a quit plan. Follow-up appointments were programmed to check in on user progress, review elements of the quit plan, answer questions, and solve issues. Quin was programmed to include teachable moments and educational content to enhance health literacy and informed decision-making. Personal agency is encouraged through exploration and self-reflection of users’ personal behaviors, experiences, preferences, and ideas.ConclusionsQuin’s successful development represents a movement toward improving access to personalized smoking cessation support. Qualitative foundations of Quin provide greater insight into the smoking cessation counseling relationship and enhances the conversational ability of the technology. The prototype chatbot will be refined through beta-testing with end users and stakeholders prior to evaluation in a clinical trial.ImplicationsOur novel study provides transparent description of the translation of qualitative evidence of real-world smoking cessation counseling sessions into the design and development of a prototype smoking cessation chatbot. The successful iterative development of Quin not only embodies the science and art of health promotion, but also a step forward in expanding the reach of tailored, evidence based, in-pocket support for people who want to quit smoking.
Racial Polarization in Attitudes towards the Criminal Legal System
Authors: Lee Karen Hanhee , Gutierrez Carmen , Pettit Becky 
Published: November 2023

AbstractExisting research often views attitudes toward the U.S. criminal legal system as reflections of punitive sentiment, overlooking racial differences in how people respond to questions related to crime and punishment. Using over four decades of nationally representative survey data from the General Social Survey, we employ latent class analysis to examine racial variation in attitudes about the U.S. criminal legal system across time. We find that among White Americans, support for increased spending to combat crime corresponds with support for harsher courts and the death penalty. In contrast, many Black Americans support increased spending on crime but oppose harsher courts and the death penalty, indicating simultaneous concern about crime and a more punitive criminal legal system. Although aggregate trends in punitiveness change similarly across race and time, we show that while preferences for punitive policies remain high among White Americans, the proportion of Black Americans who are simultaneously concerned about crime and a punitive criminal legal system rose from 14 percent in 1994 to 56 percent in 2018. These results highlight the salience of race in shaping how people evaluate the criminal legal system and draw attention to racial polarization in views on punishment and justice.
Household Debt and Social Reproduction in Everyday Life: Women’s Experiences of Caring, Agency, and Risk
Authors: Kılınçarslan Pelin 
Published: November 2023

Abstract
This article explores the ways in which everyday life produces gendered links between debt and social reproduction in contradictory ways. Based on interviews with women from indebted households in Athens and Istanbul, it argues that debt and socially reproductive work come to rely on one another, with gendered implications for caring, expanded agency, and embodied risks. Indebtedness demands forms of socially reproductive labor that women practice through caring for the debt and the indebted family. While this expands women’s agency, it also reinforces their experiences of distress and social isolation. This dual outcome reveals gendered contradictions emerging through the interdependency of debt and social reproduction. While the management of debt relies on socially reproductive labor through which women exercise greater agency, it creates embodied risks that threaten their own social reproduction, which also relies on debt.