bluuebunny/crossref_metadata_2025_split huggingface dataset:
P104 The Impact of Total Sleep Deprivation on Performance in the Esport ‘Rocket League’.
Authors: Smithies T , Toth A , Campbell M 
Published: October 2023

Abstract

Preliminary findings from this study were presented as a poster (P376) at Sleep Europe 2022 (https://doi.org/10.1111/jsr.13740).

Introduction
Sleep loss is presumed by academics and esport (organised competitive video-game play) athletes to be an in-game esport performance hindrance, primarily due to its well understood effects on multiple domains of cognitive performance. However, the effects of acute sleep loss specifically on esports performance have not been explored. We aimed to elucidate whether total sleep deprivation degrades in-game performance in the popular esport ‘Rocket League’.

Methods
Forty habitual Rocket League players were paired according to in-game ability level (twenty pairs), with one assigned to a control group (CON) and the other assigned to an intervention group (TSD). Two test sessions took place, in which paired players completed subjective and cognitive performance measures, and played seven Rocket League matches against one another. In one session, both players in a pair were well rested, while in the other, CON was rested while TSD was sleep deprived.

Results
Acute total sleep deprivation resulted in higher self-reported sleepiness, lower alertness and motivation, poorer performance on the Psychomotor Vigilance Task (PVT) and two-choice component of the Category Switch Task (CST). Despite this, overall in-game esport performance did not worsen due to sleep deprivation, although exploratory analysis suggests potential in-game strategy change.

Discussion
Our findings do not suggest that sleep loss is inconsequential for esport athletes, but rather suggest that acute bouts of sleep loss of similar/lesser severity may not be a primary concern for in-game esports performance.
Quality indicators and outcomes in ambulatory surgery
Authors: Glowka Lena , Tanella Anthony , Hyman Jaime B. 
Published: October 2023

Purpose of review
Quality indicators are used to monitor the quality and safety of care in ambulatory surgery, a specialty in which major morbidity and mortality remain low. As the demand for safe and cost-effective ambulatory surgical care continues to increase, quality indicators and metrics are becoming critical tools used to provide optimal care for these patients.

Recent findings
Quality indicators are tools used by both regulatory agencies and surgical centers to improve safety and quality of ambulatory surgical and anesthetic care. These metrics are also being used to develop value-based payment models that focus on efficient, safe, and effective patient care. Patient reported outcome measures are a growing method of collecting data on the satisfaction and postoperative recovery period for ambulatory surgical patients. Monitoring of perioperative efficiency and utilization using quality metrics are important to the financial health of ambulatory surgical centers.

Summary
Quality indicators will continue to play a growing role in the monitoring of quality and safety in ambulatory surgery, especially with the trend towards value-based reimbursement models and efficient, cost-effective surgical care. Additionally, quality indicators are useful tools to monitor postoperative patient outcomes and recovery pathways and the efficiency of operating room utilization and scheduling.
Beyond growth hormone: association of short stature types and growth hormone with scoliosis
Authors: Hong Haofeng , Hu Jiasheng , Xu Honghao , Xia Dongdong , Pan Xiangxiang , Chen Xibang , Guo Quanquan , Zhang Shuhao , Chen Jiaoxiang , Wu Yaosen , Leng Huijie , Zhang Xiaolei , Wang Xiangyang , Huang Chongan 
Published: October 2023

Study Design:
Cross-sectional and retrospective cohort study.

Objective:
We investigated the effect of three types of short stature (partial growth hormone deficiency [GHD], GHD, and idiopathic short stature [ISS]) and recombinant human growth hormone (rhGH) therapy on scoliosis.

Summary of Background Data:

In short stature, rhGH is widely used and the concentration of growth hormone varies among types. The epidemiologic characteristics of scoliosis and the role of rhGH on scoliosis remain unclear.

Methods:
A cross-sectional study was conducted among 3896 patients with short stature (partial GHD, GHD, and ISS), and a 1:1 age- and sex-matched control group with preexisting whole-spine radiographs. The cohort study included 2605 subjects who underwent radiography more than twice to assess scoliosis development, progression, and the need for bracing and surgery. Adjusted logistic regression were used to assess differences in the prevalence of scoliosis among patients with partial GHD, GHD, ISS, and controls. The Kaplan-Meier method was used to analyze the time course of scoliosis development and progression. Cox regression was applied to assess the independent factors related to scoliosis development and progression. Mendelian randomization analyses were also performed.

Results:

Compared with controls, patients with short stature had a higher incidence of scoliosis (34.47% in partial GHD, 31.85% in GHD, 32.94% in ISS vs 8.83% in control,

P

<0.001), a higher risk of scoliosis development (HR=1.964 in partial GHD,

P

<0.001; HR=1.881 in GHD,

P

=0.001; HR=1.706 in ISS,

P

=0.001), but not a higher risk of progression, brace, or surgery. Among three types of short stature, there was no differences in the incidence, development and progression of scoliosis or the need for bracing or surgery. RhGH treatment increased the risk of scoliosis development in each short stature group (HR=2.673 in partial GHD, P<0.001; HR=1.924 in GHD, P=0.049; HR=1.564 in ISS, P=0.004). Vitamin D supplementation was protective against scoliosis development (HR=0.456 in partial GHD,

P

=0.003; HR=0.42 in GHD,

P

=0.013; HR=0.838 in ISS,

P

=0.257).

Conclusions:

More attention should be paid to the spinal curve in patients with partial GHD, GHD or ISS. For short stature treated with rhGH, the risk of scoliosis development was increased. Vitamin D supplementation may be beneficial for prevention.

Level of evidence:
3.
Translation, adaptation and validation of a Spanish version of the Japanese orthopaedic association cervical myelopathy questionnaire
Authors: Ramírez Valencia Manuel , Haddad Sleiman Md , Pons Carreto Alba , García de Frutos Ana , Nuñez-Pereira Susana , PelliséUrquiza Ferran 
Published: October 2023

Study design:
Translation and psychometric testing of a questionnaire.

Objective:
Translation, adaptation and validation of the Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire (JOACMEQ) to the Spanish language.

Summary of Background data:
Degenerative Cervical Myelopathy (DCM) has a clear impact on quality of life. The Japanese Orthopaedic Association Cervical Myelopathy Evaluation Questionnaire (JOACMEQ) is a self-administered questionnaire used to assess DCM related disability and its impact on quality of life. It is compound of five domains: Cervical Function; Upper Extremity Function; Lower Extremity Function; Blader Function and Quality of Life (QoL). Despite its increasing use, the JOACMEQ has not yet been translated and validated for Spanish speaking patients.

Methods:
A total of 180 patients completed the Spanish version. Of these, 145 (80%) had DCM (mean age 62.53; SD 9.92), while 35 had neck pain without DCM (age 52.71; SD 10.29). The psychometric properties measured were: construct validity, internal consistency, reproducibility, concurrent validity and discriminatory ability.

Results:
We recruited 145 patients with DCM (mean age 62.5) and 35 with cervical pain (mean age 52.7). After Factor analysis our data showed very strong construct validity with questions strongly loaded and clustered for five factors. Internal consistency proved high (Cronbach’s α coefficient of 0.912). The ICC showed very good reproducibility for all domain (ICC range between 0.85 and 0.95). A high correlation between the JOACMEQ quality of life domain and NDI was also found (Spearman’s ρ = - 0.847, P < 0.01) confirming concurrent validity. The ROC curves proved to be significant in the upper (AUC = 0.65, P = 0.006) and lower (AUC = 0.661, P = 0.003) extremities confirming discriminatory ability.

Conclusions:
Our proposed Spanish version of the JOACMEQ retains the psychometric characteristics of the original JOACMEQ and could prove useful for the evaluation of patients with DCM in Spanish-speaking countries.
The Cognitive Profile of Elderly Patients With Mild Traumatic Brain Injury: A Role for Cognitive Reserve?
Authors: Thuss Nikki S. , Rakers Sandra E. , Bittencourt Mayra , Balart-Sánchez Sebastián A. , Spikman Jacoba M. , van der Naalt Joukje 
Published: October 2023

Objective:
To assess cognitive status in elderly patients with mild traumatic brain injury (mTBI) in the subacute phase, examine the role of cognitive reserve, and investigate associations with cognitive complaints, mental distress, and functional outcomes.

Setting:
A level 1 trauma center in the Netherlands.

Participants:
A total of 52 individuals with mTBI and 42 healthy controls.

Design:
A prospective observational cohort study.

Main Measures:
Neuropsychological assessment in the subacute phase (2 weeks to 6 months post-injury) to objectively measure the cognitive functioning, the Head Injury Symptom Checklist for subjective cognitive complaints, the Hospital Anxiety and Depression Scale for anxiety and depression, the Cognitive Reserve Index questionnaire for cognitive reserve, the Community Integration Questionnaire for community integration, and the Glasgow Outcome Scale Extended for functional outcome.

Results:
Cognitive impairments were observed in memory (P < .001) and attention, processing speed and executive control (P < .001). Cognitive reserve was not associated with neuropsychological test performance, except for one test measuring working memory. The relationship between injury severity and cognitive outcome was not moderated by cognitive reserve. Elderly patients reported significantly more complains than healthy controls regarding forgetfulness, concentration problems, and slowness. Complaints of concentration were associated with cognitive impairment. All cognitive complaints were significantly correlated with mental distress.

Conclusions:
Cognitive impairments may be present in elderly patients in the subacute phase after mTBI, and these impairments were not significantly associated with cognitive reserve. This suggests that cognitive reserve might not serve as a protective factor against the effects of mTBI in the elderly. Concentration complaints may serve as a specific indicator for cognitive impairment, while complaints of memory and mental slowness may represent more generic indicators of mental distress. These findings highlight the importance of careful screening in older adults with mTBI, guiding clinicians toward specific treatment targets encompassing cognitive impairment, diminished mental well-being, or both.
Neurophysiological oscillatory markers of hypoalgesia in conditioned pain modulation
Authors: Jin Hyerang , Witjes Bart , Roy Mathieu , Baillet Sylvain , de Vos Cecile C. 
Published: October 2023

Abstract

Introduction:
Conditioned pain modulation (CPM) is an experimental procedure that consists of an ongoing noxious stimulus attenuating the pain perception caused by another noxious stimulus. A combination of the CPM paradigm with concurrent electrophysiological recordings can establish whether an association exists between experimentally modified pain perception and modulations of neural oscillations.

Objectives:
We aimed to characterize how CPM modifies pain perception and underlying neural oscillations. We also interrogated whether these perceptual and/or neurophysiological effects are distinct in patients affected by chronic pain.

Methods:
We presented noxious electrical stimuli to the right ankle before, during, and after CPM induced by an ice pack placed on the left forearm. Seventeen patients with chronic pain and 17 control participants rated the electrical pain in each experimental condition. We used magnetoencephalography to examine the anatomy-specific effects of CPM on the neural oscillatory responses to the electrical pain.

Results:
Regardless of the participant groups, CPM induced a reduction in subjective pain ratings and neural responses (beta-band [15–35 Hz] oscillations in the sensorimotor cortex) to electrical pain.

Conclusion:
Our findings of pain-induced beta-band activity may be associated with top-down modulations of pain, as reported in other perceptual modalities. Therefore, the reduced beta-band responses during CPM may indicate changes in top-down pain modulations.
Species-specific dynamics may cause deviations from general biogeographical predictions – evidence from a population genomics study of a New Guinean endemic passerine bird family (Melampittidae)
Authors: Müller Ingo A. , Thörn Filip , Rajan Samyuktha , Ericson Per , Dumbacher John P. , Maiah Gibson , Blom Mozes , Jønsson Knud A. , Irestedt Martin 
Published: October 2023

AbstractNew Guinea, the largest tropical island, is topographically complex and is dominated by a large central mountain range surrounded by multiple smaller isolated mountain regions along its perimeter. The island is biologically hyper-diverse and harbours an avifauna with many species found only there. The family Melampittidae is endemic to New Guinea and consists of two monotypic genera:Melampitta lugubris(Lesser Melampitta) andMegalampitta gigantea(Greater Melampitta). Both Melampitta species have scattered and disconnected distributions across New Guinea in the central mountain range and in some of the outlying ranges. WhileM. lugubrisis common and found in most montane regions of the island,M. gigantaeais elusive and known from only six localities in isolated pockets on New Guinea with very specific habitats of limestone and sinkholes. In this project, we apply museomics to determine the population structure and demographic history of these two species. We re-sequenced the genomes of all seven knownM. gigantaeasamples housed in museum collections as well as 24M. lugubrissamples from across its distribution. By comparing population structure between the two species, we investigate to what extent habitat dependence, such as inM. gigantaea, may affect population connectivity. Phylogenetic and population genomic analyses, as well as acoustic differentiation, revealed thatM. gigantaeaconsists of a single population in contrast toM. lugubristhat shows much stronger population structure across the island. This work sheds new light on the mechanisms that have shaped the intriguing distribution of the two species within this family and is a prime example of the importance of museum collections for genomic studies of poorly known and rare species.
Acid Ceramidase Inhibitor LCL-805 Antagonizes Akt Signaling and Promotes Iron-Dependent Cell Death in Acute Myeloid Leukemia
Authors: Ung Johnson , Tan Su-Fern , Fox Todd E , Shaw Jeremy JP , Taori Maansi , Horton Bethany J , Golla Upendarrao , Sharma Arati , Szulc Zdzislaw M , Wang Hong-Gang , Chalfant Charles E. , Cabot Myles C , Claxton David F , Loughran Thomas P , Feith David J 
Published: October 2023

AbstractAcute myeloid leukemia (AML) is an aggressive hematologic malignancy requiring urgent treatment advancements. Ceramide is a cell death-promoting signaling lipid that plays a central role in therapy-induced cell death. Acid ceramidase (AC), a ceramide-depleting enzyme, is overexpressed in AML and promotes leukemic survival and drug resistance. The ceramidase inhibitor B-13 and next-generation lysosomal-localizing derivatives termed dimethylglycine (DMG)-B-13 prodrugs have been developed but remain untested in AML. Here, we report thein vitroanti-leukemic efficacy and mechanism of DMG-B-13 prodrug, LCL-805, across AML cell lines and primary patient samples. LCL-805 inhibited AC enzymatic activity, increased total ceramides, and reduced sphingosine levels. A median EC50 value of 11.7 μM was achieved for LCL-805 in cell viability assays across 32 human AML cell lines. As a single agent tested across a panel of 71 primary AML patient samples, a median EC50 value of 15.8 μM was achieved. Exogenous ceramide supplementation with C6-ceramide nanoliposomes, which is entering phase I/II clinical trial for relapsed/refractory AML, significantly enhanced LCL-805 killing. Mechanistically, LCL-805 antagonized Akt signaling and led to iron-dependent cell death distinct from canonical ferroptosis. These findings elucidated key factors involved in LCL-805 cytotoxicity and demonstrated the potency of combining AC inhibition with exogenous ceramide.
Upper common pathways analysis using late atrial premature depolarization in atrioventricular nodal reentry tachycardia
Authors: Park Soyoon , Park Jeong-Wook , Kim Soohyun , Kim Hwajung , Kim Sung-Hwan , Oh Yong-Seog , Choi Young 
Published: October 2023

AbstractIntroductionThe presence of an upper common pathway (UCP) in atrioventricular nodal reentrant tachycardia (AVNRT) has been suggested. However, the precise anatomical description and the actual prevalence of UCP have not been well established. We aimed to assess the prevalence of UCP in AVNRT using a late atrial premature depolarization (LAPD) maneuver.MethodsPatients who were diagnosed with typical AVNRT by electrophysiologic studies were enrolled. To evaluate the presence of UCP, a LAPD was given at the coronary sinus ostium (osCS) during AVNRT and then pacing was repeated incrementally every 10 ms. The result was interpreted as i) absence of UCP, a LAPD from osCS can reset the tachycardia without depolarizing the earliest retrograde atrial activation site (ERAS) near the proximal His; ii) presence of UCP, a LAPD from osCS can depolarize the ERAS without resetting the tachycardia; and iii) indeterminate, a LAPD from osCS either reset the ERAS and tachycardia simultaneously or does not reset both.ResultsThe LAPD maneuver was performed in 126 patients with AVNRT and one patient with nodoventricular orthodromic reentrant tachycardia (NVORT). The maneuver result demonstrated an absence of UCP in 121 (96.0%) patients, a presence of UCP in 3 (2.4%) patients, and was indeterminate in 2 (1.6%) patients. In the patient with NVORT, the result was consistent with the presence of UCP.ConclusionsThe LAPD maneuver revealed that most AVNRTs did not exhibit a single UCP. The presence of UCP was suggested in 2.4% of AVNRT cases and one case of NVORT.
Anti-CRISPR proteins trigger a burst of CRISPR-Cas9 expression that enhances phage defense
Authors: Workman Rachael E. , Stoltzfus Marie J. , Keith Nicholas C. , Euler Chad W. , Bondy-Denomy Joseph , Modell Joshua W. 
Published: October 2023

SummaryCRISPR-Cas immune systems provide bacteria with adaptive immunity against bacteriophages, but they are often transcriptionally downregulated to mitigate autoimmunity. In some cases, CRISPR-Cas expression increases in response to a phage infection, but the mechanisms of induction are largely unknown, and it is unclear whether induction occurs strongly and quickly enough to benefit the bacterial host. InS. pyogenes, Cas9 is both an immune effector and autorepressor of CRISPR-Cas expression. Here, we show that phage-encoded anti-CRISPR proteins relieve Cas9 autorepression and trigger a rapid increase in CRISPR-Cas levels during a single phage infective cycle. As a result, fewer cells succumb to lysis leading to a striking survival benefit after multiple rounds of infection. CRISPR-Cas induction also reduces lysogeny, thereby limiting a route for horizontal gene transfer. Altogether, we show that Cas9 is not only a CRISPR-Cas effector and repressor, but also a phage sensor that can mount an anti-anti- CRISPR transcriptional response.
Disparate pathways for extrachromosomal DNA biogenesis and genomic DNA repair
Authors: Rose John C. , Wong Ivy Tsz-Lo , Daniel Bence , Jones Matthew G. , Yost Kathryn E. , Hung King L. , Curtis Ellis J. , Mischel Paul S. , Chang Howard Y. 
Published: October 2023

ABSTRACTOncogene amplification on extrachromosomal DNA (ecDNA) is a pervasive driver event in cancer, yet our understanding of how ecDNA forms is limited. Here, we couple a CRISPR-based method for induction of ecDNA with extensive characterization of newly formed ecDNA to examine ecDNA biogenesis. We find that DNA circularization is efficient, irrespective of 3D genome context, with formation of a 1 Mb and 1.8 Mb ecDNA both reaching 15%. We show non-homologous end joining and microhomology mediated end joining both contribute to ecDNA formation, while inhibition of DNA-PKcs and ATM have opposing impacts on ecDNA formation. EcDNA and the corresponding chromosomal excision scar form at significantly different rates and respond differently to DNA-PKcs and ATM inhibition. Taken together, our results support a model of ecDNA formation in which double strand break ends dissociate from their legitimate ligation partners prior to joining of illegitimate ends to form the ecDNA and excision scar.SIGNIFICANCEOur study harnesses a CRISPR-based method to examine ecDNA biogenesis, uncovering efficient circularization between DSBs. ecDNAs and their corresponding chromosomal scars can form via NHEJ or MMEJ, but the ecDNA and scar formation processes are distinct. Based on our findings, we establish a mechanistic model of excisional ecDNA formation.
CSR initiatives in SMEs: employee perception and their influence on employment decisions
Authors: Marcinkowska Elżbieta , Sawicka Joanna 
Published: October 2023

PurposeNearly half of the surveyed SMEs in Poland admitted that there is a very strong competition on the market where they operate. Among the neuralgic factors they point to the lack of qualified employees (PARP, 2021). Companies can use CSR policies to attract competent employees and retain valuable ones. Therefore, the purpose of this research paper is to find out whether, according to employees working in SME companies, an active CSR policy influences their employment-related decisions.Design/methodology/approachThe data were collected through questionnaires received from 618 employees of 29 SMEs in Poland through questionnaires, which were analyzed with the IBM SPSS Statistics 26.0 and Microsoft Excel 2019.FindingsThe survey results provide evidence that CSR activities are an important factor in employees' decisions about potential employment and/or continued employment. In particular, the results show that almost all areas of CSR, except cooperation with the local communities, are important to employees. The survey also provides a clear answer as to which CSR initiatives benefiting employees of SME companies are the most important for them.Originality/valueThe conducted research fills a gap in CSR related studies on the SME sector in Poland. This is important, given the significant share of SME sector companies in the market in Poland and around the world.