bluuebunny/crossref_metadata_2025_split huggingface dataset:
Su(Hw) interacts with Combgap to establish long-range chromatin contacts
Authors: Vorobyeva Nadezhda E. , Krasnov Alexey N. , Erokhin Maksim , Chetverina Darya , Mazina Marina 
Published: January 2024

Abstract
Background
Insulator-binding proteins play a critical role in genome architecture by forming and maintaining contact domains. While the involvement of several IBPs in organising chromatin architecture in Drosophila has been described, the specific contribution of the Suppressor of Hairy wings (Su(Hw)) insulator-binding protein to genome topology remains unclear.
Results
In this study, we provide evidence for the existence of long-range interactions between chromatin bound Su(Hw) and Combgap, which was first characterised as Polycomb response elements binding protein. Loss of Su(Hw) binding to chromatin results in the disappearance of Su(Hw)-Combgap long-range interactions and in a decrease in spatial self-interactions among a subset of Su(Hw)-bound genome sites. Our findings suggest that Su(Hw)-Combgap long-range interactions are associated with active chromatin rather than Polycomb-directed repression. Furthermore, we observe that the majority of transcription start sites that are down-regulated upon loss of Su(Hw) binding to chromatin are located within 2 kb of Combgap peaks and exhibit Su(Hw)-dependent changes in Combgap and transcriptional regulators’ binding.
Conclusions
This proof-of-concept study demonstrates that Su(Hw) insulator binding protein can form long-range interactions with Combgap, Polycomb response elements binding protein, and that these interactions are associated with active chromatin factors rather than with Polycomb dependent repression.
Evaluation of a financial incentive intervention on malaria prevalence among the residents in Lake Victoria basin, Kenya: study protocol for a cluster-randomized controlled trial
Authors: Matsumoto Tomoya , Nagashima Masaru , Kagaya Wataru , Kongere James , Gitaka Jesse , Kaneko Akira 
Published: January 2024

Abstract
Background In the Lake Victoria basin of western Kenya, malaria remains highly endemic despite high coverage of interventions such as mass distribution of long-lasting insecticidal nets (LLIN), indoor residual spraying (IRS) programs, and improvement of availability and accessibility of rapid diagnostic tests (RDT) and artemisinin-based combination therapy (ACT) at community healthcare facilities. We hypothesize that one major cause of the residual transmission is the lack of motivation among residents for malaria prevention and early treatment.
Methods This study will aim to develop a demand-side policy tool to encourage local residents’ active malaria prevention and early treatment-seeking behaviors. We examine the causal impact of a financial incentive intervention complemented with malaria education to residents in malaria-prone areas. A clusterrandomized controlled trial is designed to assess the effect of the financial incentive intervention on reducing malaria prevalence in residents of Suba South in Homa Bay County, Kenya. The intervention includes two components. The first component is the introduction of a financial incentive scheme tied to negative RDT results for malaria infection among the target population. This study is an attempt to promote behavioral changes in the residents by providing them with monetary incentives. The project has two different forms of incentive schemes. One is a conditional cash transfer (CCT) that offers a small reward (200 Ksh) for noninfected subjects during the follow-up survey, and the other is a lottery incentive scheme (LIS) that gives a lottery with a 10% chance of winning a large reward (2,000 Ksh) instead of the small reward. The second component is a knowledge enhancement with animated tablet-based malaria educational material (EDU) developed by the research team. It complements the incentive scheme by providing the appropriate knowledge to the residents for malaria elimination. We evaluate the intervention's impact on the residents' malaria prevalence using a cluster-randomized control trial.
Discussion A policy tool to encourage active malaria prevention and early treatment to residents in Suba South, examined in this trial, may benefit other malaria-endemic counties and be incorporated as part of Kenya's national malaria elimination strategy.
Trial registration: UMIN000047728, registered on 29th July 2022
Analysing the Enablers of Green Supply Chain Management: FDM and ISM Approach
Authors: Sharma Mukesh , Sohani Nagendra , Agarwal Sachin 
Published: January 2024

Abstract

This study aims to identify and analyse the green supply chain management enablers (GSCMEs) in the field of manufacturing industries in recent scenario. GSCMEs are identified using literature review and brainstorming session conducted with the experts. Fuzzy Delphi Method (FDM) applied to accept relevant GSCMEs, Interpretive Structural Modelling (ISM) applied to analyse the relationship among GSCMEs and Matriced’ Impacts Croise´s Multiplication Applique´ea´ un Classement (MICMAC) analysis categories GSCMEs on the basis of driving and dependence power. Research findings suggest that “Top management supports” and “GSCM based strategic planning” are the important GSCMEs in the efficient and effective implementation of green supply chain management (GSCM). This study provides relevant information to all GSCM practitioners regarding implementation of green action plan and environment regulations in industries. The research work assist stakeholders to identify higher driving power GSCMEs in the manufacturing industries for effective implementation of GSCM.
Environment and Wellbeing: Assessment of Quality of Life Through Vegetation Index in Guanambi's Alvorada Neighborhood, Bahia – Brazil
Authors: Vieira Gabriel Soares , Rodrigues Sabrina Dias , Silva Ingrid Layla Sousa , de Oliveira Maria Eduarda Teixeira , De Brito Gabriella Raiany Teixeira , Dias Felipe Teixeira 
Published: January 2024

Abstract

The presence of vegetation in urban spaces is a crucial indicator for describing the quality of life of city inhabitants. The lack of green areas can contribute, primarily, to an increase in the sensation of thermal discomfort during periods of intense heat, negatively impacting the health and comfort of residents. Thus, the aim of this article is to investigate the intrinsic relationship between the vegetative environment and human well-being, with a specific focus on the community of Alvorada Neighborhood in the municipality of Guanambi, Bahia. The evaluation of green areas was conducted based on the vegetation coverage index (VCI), population density, aerial photograph analysis, and the percentage of vegetation established by institutions, aligning with Sustainable Development Goals (SDGs) 3 (good health and well-being), 11 (sustainable cities and communities), and 13 (climate action). Accordingly, this article sought to deepen the understanding of the relationships between the urban vegetation index and the quality of life of local residents, aiming to stimulate concrete actions and positive reflections in the Alvorada Neighborhood community. Therefore, the study addressed the delimited area in a way that provided the necessary foundation to highlight that the Vegetation Coverage Index (VCI) has a significant impact on the overall state of the population.
Hsa_circ_0079474 facilitates epithelial-mesenchymal transition in intrauterine adhesion via miR-630/YAP1 axis
Authors: Xing Chen , Zhou Yan , Wang Jiwen , Song Zhenzhen , Yang Jing , Xu Wei , Zhu Danxuan , Sun Ying , Sun Xiaohong , Hu Siwen , Jiang Ziwei , Qiu Yixuan , Ji Mengying , Li Yujing , Zhou Xuan , Zhao Yue , Lu Yihan , Yuan Shuning , Fang Qin , Han Nannan , Zhou Jingwei , Ji Tonghui , Dai Huihua , Ding Wei 
Published: January 2024

Abstract
Insufficient understanding exists of the molecular mechanisms underlying circRNA involvement in IUA and requires further investigation. This research aims to examine the role of hsa_circ_0079474 (circDGKB-009) and its potential mechanisms in intrauterine adhesion (IUA). A circRNA microarray was utilized to identify differences in circRNA expression between fibrotic endometrial samples and normal endometrial samples. Subsequent studies confirmed the expression and biological functions of hsa_circ_0079474 both in vivo and in vitro using various experimental techniques such as CCK-8, EdU, flow cytometry, FISH, RT-PCR, Western blot and IHC/ICC. The interactions between hsa_circ_0079474 and miR-630, as well as miR-630 and YAP1 were determined using dual-luciferase reporter assay and RNA immunoprecipitation. Hsa_circ_0079474 was dramatically elevated in IUA tissues compared to normal tissues. Hsa_circ_0079474 was found to enhance cell proliferation, expedite cell cycle progression, and facilitate epithelial-mesenchymal transition (EMT). Mechanistically, hsa_circ_0079474 acted as a sponge for miR-630, resulting in upregulation of YAP1 expression. This, in turn, promoted the progression of IUA. Hsa_circ_0079474 improves IUA by regulating the miR-630/YAP1 axis, providing a novel understanding of the molecular mechanisms underlying circRNA in IUA.
The relationship between human papillomavirus genotype distribution and age and the association with high-grade squamous intraepithelial lesions of the cervix or cervical cancer in Jilin Province, China
Authors: Zhu Lixia , Li Peiyao , Wang Yingyao , Zhang Juping , Chen Juan , Ren Jing , Wu Yitong , Yao Yanjin , Wang Ying , Sun Jing , Hua Yafang , Li Ping , Liu Qin 
Published: January 2024

Abstract
Human papillomavirus (HPV) infection is related to the occurrence of cervical cancer. We enrolled 21,282 individuals, including 634 males and 20,648 females, in Jilin Province, China, from October 2017 to September 2019. Significant variations were observed in the prevalence of HPV types 16, 18, 31, 33, 52, 56, 58, 59, 66, 53, 6, and 11 across different age groups (P < 0.05). The occurrence of HPV infection was considerably greater among females than among males. Additionally, a noteworthy association was found between HPV16, 18, 33, and 58 infections and the presence of high-grade squamous intraepithelial lesions or cervical cancer in females. This relationship exhibited a U-shaped relationship with age. Our study might play a role in guiding women of different ages to get tested for HPV infections and determine the right HPV vaccine in relation to a woman's age.
Growing Deviations Between Elite and Non-Elite Media Coverage of Climate Change: The American Story
Authors: Bolstad Parker , Victor David G. 
Published: January 2024

Abstract
Empirical research aimed at understanding public awareness and opinion on climate change has focused heavily on media coverage. Nearly all prior media studies focus on the United States and on a small number of elite news sources, including the national newspapers of record. Here we take advantage of a database (MediaCloud) that covers a much larger array of print and word media: 168 million articles about all subjects, derived from 9000 unique U.S. news sources. Coverage of climate change from these “heartland” sources—dominated by state and local news outlets far from the headquarters of national newspapers of record—has risen 144% from 2011 until 2022. Elite news coverage, however, has risen at twice that pace (299%). Both news sources show spikes in coverage related to similar events (e.g. negotiation of the Paris Accords or President Trump’s withdrawal from Paris); both see a decline in coverage when other events become more prominent (e.g., the first COVID-19 cases). Heartland news sources pay close attention to events such as the discussion of climate change by Pope Francis and to right-wing media campaigns; elite news sources are more focused on climate conferences and extreme weather. Over time, the propensity to cover climate change has diverged. In 2011 there were 104 days when the heartland news sources had more coverage of climate change than elite news outlets such as The New York Times and The Wall Street Journal. By 2022 there were only 11 such days. That year, elite news outlets produced roughly three times the coverage of climate change as heartland news outlets, most of which cover state and local matters.
Fluorescent non-canonical amino acid provides mechanistic insight into the human serotonin transporter
Authors: Nygaard Andreas , Zachariassen Linda , Larsen Kathrine , Kristensen Anders , Loland Claus 
Published: January 2024

Abstract

The serotonin transporter (SERT), responsible for the reuptake of released serotonin, serves as a major target for antidepressants and psychostimulants. Nevertheless, refining the mechanistic models for SERT remains challenging. Here, we bridge the structural and functional understanding of SERT by incorporating the fluorescent non-canonical amino acid Anap through genetic code expansion. We were able to elucidate the steady-state and time-resolved conformational dynamics of purified SERT with Anap genetically encoded at the intracellular- or extracellular site. This uncovered the competitive mechanisms underlying cation binding and assigned unique binding- and allosteric coupling signatures for several inhibitors and substrates. Finally, we tracked in real-time the conformational transitions in response to the interaction with Na+ and substrate. Our methodological platform offers an unprecedented spatiotemporal resolution which enables us to resolve novel mechanistic features of SERT.
Mediating effect of resilience and job embeddedness on the relationship between organisational identification and turnover intention among junior nurses: a cross-sectional study
Authors: Liu Nana , Li Guopeng , Zhou Fang , Hu Huihui , Yang Yi , Sun Xinfeng 
Published: January 2024

Abstract
Background
The role of junior nurses in healthcare services has been emphasised in China, where a high turnover rate among junior nurses has been observed. Junior nurses’ turnover intentions have garnered increasing attention in further research to determine the factors that keep them with their employers. This study aimed to investigate the relationship between Organisational Identification (OID), resilience, job embeddedness, and turnover intention among Chinese junior nurses based on social identity and conservation of resources (COR) theories.
Methods
This cross-sectional study comprising 602 junior nurses was conducted at four tertiary hospitals in Shandong Province, China, and the data were analysed using descriptive statistics, independent-sample t-tests, one-way Analysis of Variance (ANOVA), Spearman’s correlation analysis, regression analysis, and bootstrapping.
Results
Significant correlations were found between OID, resilience, job embeddedness, and turnover intention. Analysis of mediating effects revealed that OID predicted turnover intention through three indirect pathways: the mediating effect value of resilience was − 0.033 [Bootstrap 95% CI: -0.057, -0.010], accounting for 16.26% of the total effect; the mediating effect value of job embeddedness was − 0.080 [Bootstrap 95% CI: -0.114, -0.054], accounting for 39.41% of the total effect; and the chain mediating effect value of resilience and job embeddedness was − 0.036 [Bootstrap 95% CI: -0.051, -0.023], accounting for 17.73% of the total effect.
Conclusions
OID not only directly predicts turnover intention but also exerts indirect predictions through the separate and chain mediating effects of resilience and job embeddedness. OID can reduce junior nurses’ turnover intention by amplifying the accumulation of personal resources. Hospital administrators should incorporate OID, resilience, and job embeddedness into the routine psychological screening of junior nurses and implement essential comprehensive strategies aimed at enhancing OID, bolstering resilience, and promoting job embeddedness, to mitigate junior nurses’ turnover intentions.
Unveiling the Anticancer Mechanisms of Prodigiosin by inhibiting of CDK1, TOP2A, and AURKB Expression in Cervical Carcinoma
Authors: Zhu Zhenkun , Jiang Chunfan , Xiang Chunxiang , Chen Qianbao , Yang Mei , Tang Mengjun , Xing Hui 
Published: January 2024

Abstract
Prodigiosin (PG) demonstrates a selective targeting effect on tumor cells. However, its role in cervical carcinoma is still being studied. In this study, we aim to study the specific targets and mechanism of PG in cervical carcinoma. We employed GO enrichment and KEGG analysis to identify core genes in CC patients. To corroborate the expression levels of these core genes, we used staining and RT-PCR on both normal and tumor tissues. Following this, the specific effects of PG on Hela, H8, and A549 cells were compared. After PG treatment, cell viability was evaluated using a CCK8 assay at various PG concentrations. Apoptosis in Hela cells was determined through flow cytometry post-PG treatment, and the expression of target genes was measured via RT-PCR. Our analysis highlighted CDK1, TOP2A, and AURKB emerging as core genes. The expression of CDK1, TOP2A, and AURKB, both at the protein and gene levels, was found to be higher in cervical carcinoma tissues compared to controls. Furthermore, lower PG concentrations diminished the viability of Hela and A549 cells without significantly impacting H8 cells. PG was observed to induce apoptosis in Hela cells by reducing the expression of CDK1, TOP2A, and AURKB genes.
Use of dental care services among adolescents living with HIV on Antiretroviral Treatment in Kampala, Uganda: a cross-sectional study
Authors: Nakyonyi Maria Gorretti , Birungi Nancy , Mwesigwa Catherine Lutalo , Åstrøm Anne Nordrehaug 
Published: January 2024

Abstract
Objective
The main purpose of this study to assess the prevalence and socio-behavioural determinants of ever-use of dental care services among the adolescents aged 10–18 years, living with HIV on Antiretroviral treatment (ART), attending selected HIV clinics in Kampala, Uganda.
Methods
A cross-sectional study was carried out between March and September 2020. The study conveniently recruited 154 adolescents between 10–18 years from 4 specific HIV clinics in Kampala. The Andersen’s behavioral model guided the selection of variables in terms of ever use of dental care services as the outcome- and predisposing, enabling, need related factors and dental health related behavior as exposure variables. Data was analyzed using Fischer’s exact test for cross-tabulation and modified Poisson regression for multivariate analysis.
Results
The prevalence of ever-use of dental care services was 12.3%. The adolescents aged 14–18 years were more likely to have used dental care services (Prevalence ratio (PR) of 3.35 (Confidence Interval (CI) 1.48–7.59) than those aged 10–13 years. Fear of spread of HIV was negatively associated with ever-use of dental care services (PR of 0.06 and CI of (0.01–0.44). Participants who were afraid of going to the dentist were more likely to have ever used dental care services (PR of 2.98 and CI of 1.41–6.30) than those not afraid. Failure to receive dental treatment because it was not part of the medical appointment had a positive association with ever-use of dental care services (PR of 4.50 (CI: 1.14–17.80). Those who were satisfied with their dental condition were less likely to have ever-used dental care services (PR of 0.21 and CI of (0.05–0.94). Bad oral odor was positively associated with ever-use of dental care services with a PR of 2.80 and CI of 1.19–6.60. Use of soap for toothbrushing was positively associated with ever-use of dental care services (PR of 2.51, CI of 1.47–4.28).
Conclusion
The study found low frequency of dental care use among HIV infected adolescents in Kampala, Uganda, with age being a predisposing factor. Enabling factors included fear of HIV spread, dental appointment failure, and satisfaction with dental condition and bad oral odor while under personal oral hygiene and dental practices, use of soap for toothbrushing was an important association of use of dental care.
Advancing unmanned surface vehicle heading control: real-sea validation of a practical model predictive controller for a medium-scale hybrid-power trimaran
Authors: Wang Jian , Wang Jun , Liang Xiaofeng , Liu Junjie , Cheng Shunzhao , Yi Hong 
Published: January 2024

Abstract
The development of medium- to large-scale, high-performance, and environmentally-friendly unmanned surface vehicles (USVs) is a burgeoning trend in intelligent marine systems. The heading controller is crucial for USVs to execute various missions, especially given their inherent underactuation characteristics. Despite extensive exploration of numerous control strategies, the majority of studies have been confined to smaller, conventional USVs with an emphasis on simulation validation. This limitation stems from the significant gap between theory and practical engineering. We here introduce a model predictive controller for the heading control of a medium-scale hybrid-power unmanned trimaran and validate its performance in real sea. This controller directly accounts for the amplitude and increment constraints of the water-jet thrust angle, and its asymptotic stability is established with a terminal cost. Experimental results demonstrate that the controller’s ability to effectively track and maintain constant desired heading angles without any disturbance compensation, surpassing the traditional proportional-integral-derivative controller in terms of response speed, overshoot, steady-state error, and robustness. We further showcase the successful application of this controller in path-following missions, evidencing its precise adherence to desired paths. A critical aspect of our research is monitoring the controller’s execution time in all experiments, affirming its real-time response capabilities given the onboard hardware’s limited computational power. This research not only contributes a significant advancement in the field of USV control but also provide a new solution for the integration of theoretical control strategies into practical marine engineering.
Sexual Health Literacy Confidence Among Urology Resident Physicians
Authors: Horwitz Rainey , Lewis Kevin , Khooblall Prajit , Haile Eiftu , Lundy Scott , bajic Petar 
Published: January 2024

Abstract
Introduction: Despite the importance of sexual health, physicians may not be adequately trained and equipped to diagnose and treat sexual dysfunction. It is crucial that urologists are trained to ask detailed questions about sexual health, counsel on healthy sexual habits, and appropriately identify and treat sexual dysfunction.
Objective: To determine the level of comfort among urology residents in taking a comprehensive sexual health history, identifying sexual dysfunction, and treating patients of various gender identities and sexual orientations.
Methods: An anonymous electronic survey was distributed to current urology residents in the United States. The survey contained five demographic questions and twenty-three questions tied to previously established sexual health competency domains.
Results: A total of 52 urology residents participated with post-graduate years ranging from one to six. 46.3% reported having any formal sex education during residency. 27% felt comfortable answering questions about anal stimulation devices and 35% felt comfortable answering questions about vaginoclitoral stimulation devices. Greater than 94% of residents felt “very comfortable” or “somewhat comfortable” with describing cisgender male sexual dysfunction and performing a physical exam for a cisgender male with sexual dysfunction, but 59% felt the same towards cisgender female sexual dysfunction. When counseling transgender men or women on sexual practices, therapies, or surgeries, 29% felt comfortable.
Conclusion: There are knowledge gaps in sexual health literacy confidence among urology residents surveyed. Women and sexual and gender minority patients may be at higher risk. Sexual health education optimization during urology residency has the potential to promote improved understanding of sexual health and evaluation and management of sexual dysfunction in general.