bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adult skull bone marrow is an expanding and resilient hematopoietic reservoir
Authors: Adams Ralf , Koh Bong-Ihn , Mohanakrishnan Vishal , Jeong Hyun-Woo , Park Hongryeol , Choi Young Jun , Nieminen-Kelhä Melina , Pereira Raquel , Adams Susanne , Lee Hyuek Jong , Bixel M. Gabriele , Vajkoczy Peter , Krause Daniela 
Published: October 2023

Abstract

The bone marrow microenvironment is a critical regulator of hematopoietic stem cell self-renewal and fate. While it is appreciated that aging, chronic inflammation and other insults compromise bone marrow function and thereby negatively affect hematopoiesis, it is not known whether different bone compartments exhibit distinct microenvironmental properties and functional resilience. Here, we have employed imaging, pharmacological approaches, and mouse genetics to uncover specialized and highly surprising properties of bone marrow in adult and aging skull. Specifically, we show that the skull bone marrow undergoes lifelong expansion involving vascular growth, which results in an increasing contribution to total hematopoietic output. Furthermore, skull is largely protected against major hallmarks of aging, including upregulation of inflammatory cytokines, adipogenesis and loss of vascular integrity. Striking dynamic and rapid changes to the skull vasculature and bone marrow are induced by physiological alterations, namely pregnancy, but also pathological challenges, such as stroke and experimental chronic myeloid leukemia. These responses are highly distinct from femur, the most extensively studied bone marrow compartment. We propose that skull harbors a protected and dynamically expanding bone marrow microenvironment, which is relevant for experimental studies but, potentially, also clinical treatments in humans.
Diversity and spatial genetic structure of the common opossum (Didelphis marsupialis) in an urban matrix of Northwestern Colombia
Authors: Henao-Sáenz Carolina , Herrera-Pérez Juliana , Soto-Calderón Iván Darío 
Published: October 2023

Abstract

The common opossum is a marsupial widely distributed in the Neotropics, where it lives in urbanized environments. The apparent scarcity and fragmentation of available habitat, and high rates of vehicle collisions, may represent barriers to dispersal. To assess the functional connectivity of this species and its potential use as a model in urban ecology, we evaluated the structure and genetic diversity of opossums in the metropolitan area of the Aburrá Valley (AMVA), Colombia, using sequences of the mitochondrial Cytochrome B (Cytb) gene and nine autosomal microsatellite loci. Although Cytb presented low levels of variation, microsatellite markers revealed high genetic diversity (He = 0.852 and Ho = 0.698). The geographic distribution of mitochondrial lineages and a spatial principal component analysis (sPCA) of microsatellites showed the absence of genetic structure and effective barriers to dispersal of opossums in the study area. This scenario suggests that hostile landscapes such as those in urban environments may be suitable enough to allow the effective connectivity of some species such as D. marsupialis and others with high dispersal ability in urbanized areas. Nonetheless, it is necessary to evaluate the impact of releases of rescued fauna made by the environmental authority on the population structure of urban opossums.
Perception of Faculty in the Community Health Sciences Colleges towards Simulation-based Education in Clinical Nutrition Undergraduate Practical Courses: A National-level, Observational Study in Saudi Arabia
Authors: Dayel Iman Bin , Alahmad Shahad 
Published: October 2023

Abstract
Background
Simulation in medical healthcare practice and education has been applied since the early twentieth century. Simulation now is widely used for training and education in different fields including healthcare education. Medicine and healthcare students can be trained in a secure, efficient, and engaging setting by Simulation-based Education (SBE). Therefore, this study aimed to assess the perception of faculty members in the community health departments towards SBE to be used in practical subjects for clinical nutrition undergraduate courses.
Method
This national-level observational study was conducted among community health sciences faculty members. The perception was assessed using a questionnaire that included three sections; the first section included socio-demographic questions, the second section included questions to assess teachers’ perceptions of SBE, and the last section was an open-ended question to explore perceived barriers towards SBE.
Results
This questionnaire was completed by 125 faculty members, of whom 36 (28.8%) were male, and 89 (71.2%) were female. Overall, faculty members had positive perception, with a mean score of 3.86 ± 0.74, but high level of anxiety toward SBE, with a mean score of 3.42 ± 0.75. There was a statistically significant difference between the responses of the faculty members based on their received training in simulation (P = 0.001).
Conclusion
The study results indicate that community health sciences faculty members’ perception of SBE in Saudi Arabia is generally positive. However, the results show high levels of anxiety among faculty members toward SBE.
Structural insights into ligand recognition and activation of melanin-concentrating hormone receptor MCHR1
Authors: Gong Weimin , Liu Haiping , Ye Xiaofan , Liu Guibing , Li Xiu 
Published: October 2023

Abstract
Melanin-concentrating hormone (MCH) is a 19-amino acid cyclic neuropeptide playing a crucial role in energy homeostasis, sleep, and other physiological functions. In rodents and humans, MCH promotes feeding through the G protein-coupled receptor MCHR1, making it a promising therapeutical target for metabolic disorders. However, mechanisms for ligand recognition and activation of MCHR1 remains obscure due to the lack of structural information. In this study, we report the cryo-EM structures of MCHR1-Gi1 complex in the MCH-bound state and the ligand-free state. We revealed the specific recognition mechanism of MCH by MCHR1 as well as multiple conformational states of the signaling complex. Distinct distribution of different states in the presence and absence of MCH highlights the relevance of conformational tuning of the complex in MCHR1 activation. Our study provides structural insights into ligand recognition and activation mechanisms of MCHR1 and will facilitate the development of drugs targeting the MCH system.
Application of Optimized Kalman Filtering in Target Tracking Based on Improved Gray Wolf Algorithm
Authors: Pang Zheming , Wang Yajun , Yang Fang 
Published: October 2023

Abstract

High precision is a very important index in target tracking. In order to improve the prediction accuracy of target tracking, an optimized Kalman filter approach based on improved Gray Wolf algorithm (IGWO-OKF) is proposed in this paper. Since the convergence speed of traditional Gray Wolf algorithm is slow, meanwhile, the number of gray wolves and the choice of the maximum number of iterations has a great influence on the algorithm, a nonlinear control parameter combination adjustment strategy is proposed. An improved Grey Wolf Optimization algorithm (IGWO) is formed by determining the best combination of adjustment parameters through the fastest iteration speed of the algorithm. The improved Grey Wolf Optimization algorithm (IGWO) is formed, and the process noise covariance matrix and observation noise covariance matrix in Kalman filter are optimized by IGWO. The proposed approach is applied into. The experiment results show that the proposed IGWO-OKF approach has low error, high accuracy and good prediction effect.
Obstacles Avoidance Charging Schedule for Multiple Mobile Charging Vehicles in Wireless Rechargeable Sensor Networks
Authors: Raha Sk Md Abidar , Azharuddin Md , Kuila Pratyay 
Published: October 2023

Abstract
The integration of wireless power transfer technology into mobile charging vehicles (MCVs) opens up new possibilities for wirelessly recharging the batteries of sensor nodes (SNs). Thereby, it extends the network’s operational lifespan for wireless rechargeable sensor networks (WRSNs). However, the task of devising optimal charging schedules with MCVs is a complex one, especially when these MCVs encounter obstacles necessitating detours. These detours can impact the overall energy consumption and longevity of WRSNs. In this article, we introduce a strategic approach that harnesses the capabilities of multiple MCVs to enhance the efficiency of charging operations within WRSNs. The proposed method takes into account several key factors, such as the temporal and spatial aspects of charging requirements, the presence of multiple MCVs, and the challenges posed by obstacles. By amalgamating these elements into a unified metric, our strategy determines charging sequences based on collective charging preferences. The allocation of charging partitions is facilitated by clustering, leveraging the locations of SNs and their energy consumption rates, with each MCV tending to a designated partition. Furthermore, we employ an obstacle avoidance algorithm grounded in the use of anchor points and projection points to address scheduling issues when obstacles are encountered. Overall, this strategic framework offers an efficient and effective solution for SN charging, contributing to improved longevity and performance within WRSNs. Extensive simulations are conducted to validate the proposed methodology, and performance comparisons with existing systems are provided. An analysis of variance (ANOVA) is performed, followed by a post-hoc analysis.
An Exploration of COVID-19 Vaccination Models for Refugees and Newcomer Immigrants in a Canadian City
Authors: Aghajafari Fariba , Ness Alyssa , Wall Laurent , Weightman Amanda , Lake Deidre , Anupindi Krishna , Moorthi Gayatri , Kuk Bryan , Santana Maria , Coakley Annalee 
Published: October 2023

Abstract
Background
The World Health Organizations stresses the need for tailored COVID-19 models of vaccination to meet the needs of diverse populations and ultimately reach high rates of vaccination. However, little evidence exists on how COVID-19 models of vaccination operated in the novel context of the pandemic, how vulnerable populations, such as refugees, experience COVID-19 vaccination systems in high-income countries, and what lessons may be learned from vaccination efforts with vulnerable populations. To address this gap, this study explored COVID-19 vaccine delivery models available to refugees and newcomers, and refugee experiences across different COVID-19 vaccine delivery models in Calgary, Canada, and surrounding area in 2021 and 2022, to understand the barriers, strengths, and strategies of models to support access to COVID-19 vaccination for newcomers (with a focus on refugees).
Methods
Researchers conducted structured interviews with Government Assisted Refugees (n = 39), and semi-structured interviews with Privately Sponsored Refugees (n = 6), private refugee sponsors (n = 3), and stakeholders involved in vaccination systems (n = 13) in 2022. Thematic analysis was conducted to draw out themes related to barriers, strengths, strategies of vaccine delivery models, and intersections with patient experiences.
Results
Newcomer-specific and mainstream vaccination models were explored. They demonstrated how partnerships between organizations, multi-pronged approaches, and culturally responsive services were crucial to navigate ongoing and emergent factors, such as vaccine hesitancy, mandates, and determinants of under-vaccination. Many vaccination models were not specific to refugees and included newcomers and established immigrants.
Conclusions
Increasing COVID-19 vaccine uptake for refugees and newcomers is complex and requires trust, ongoing information provision, and local partnerships to address ongoing and emerging factors. Three key policy implications were drawn. First, findings demonstrated the need for flexible funding to offer outreach, translation, cultural interpretation, and to meet the basic needs of patients prior to engaging in vaccinations. Second, the research showed that embedding culturally responsive strategies within services ensures community needs are met. Finally, collaborating with partners that reflect the diverse needs of communities is crucial for the success of any health efforts serving newcomers.
Mitochondrial DNA variation of Algerian honey bees Apis mellifera intermissa and Apis mellifera sahariensis based on COI-COII RFLP and sequencing
Authors: Attia saliha Salem , Chahbar Nora , Abed Fouzia , Oussalah Narimen , Kandemir Irfan , Ainouche Abedelkadder , Doumandji salaheddine 
Published: October 2023

Abstract
To investigate the diversity among Algerian honeybees, we analyzed the mitochondrial DNA of 92 individuals originating from 18 different locations in North and South of Algeria based on the E2-H2 amplification of COI-COII intergenic region. Additional to RFLP analysis of mtDNA COI-COII intergenic region by using the restriction enzyme Dra I, we also sequenced 50 honeybee individuals displaying different restriction enzyme profiles. Our data revealed the presence of four different mtDNA haplotypes at various frequencies (A8, A9, C2 and M4). Based on the haplotype diversity, we can infer that the Algerian honeybees surveyed mainly consists of two haplotypes namely A8 (30.48%) and A9 (63.41%), the latter being most frequent in native regions of Saharan honeybee Apis mellifera sahariensis (EL Bayadh, Naama and Bechar) with a high frequency value (85.71%) comparative to the rest of the country (52.72% of frequency). Moreover, a low genetic introgression (6.09%) of non-local mtDNA haplotypes (C2 and M4) was also observed, which may be explained by the importation of foreign honeybee queens to Algeria. The sequencing of 50 individuals revealed the presence of 15 new haplotypes not previously described (6 sequences of A9, 6 sequences of A8, 2sequences of C2 and 1 sequence of M4), which are available in NCBI. Moreover the phylogenetic analysis revealed that the A8 haplotypes form a well-supported monophyletic group, which appear as a derived branch from the A9 group. This suggests that the haplotypes A8 have likely recently diverged within the A. mellifera intermissa complex in Northern (tellian) regions. Implications for insect conservation: This study help to more understand the genetic diversity which is essential for the description and the conservation of Algerian honeybee population.
Evaluating Seismic Stability of Retaining Wall Heel Strengthened with Pre- Stressed Ropes using Kinematic Limit Analysis Approach
Authors: Peng Tao , Zhang Xiaoxi , Huang Xiaoqiang , Yin Pingbao , Ren Dongxing 
Published: October 2023

Abstract
To enhance the resistance against sliding and overturning in a cantilever retaining wall, the study introduces a heel plate at the base of the wall’s backside. This heel plate is designed to work with the wall's combined weight and the soil fill's weight placed on the heel plate. These components work together to mitigate the lateral forces produced by the soil fill. The objective is to efficiently and cost-effectively mitigate the seismic forces by integrating a cantilever retaining wall with a heel plate and pre-stressed rope, capitalising on the favourable seismic response characteristics. The study used the kinematical approach based on the upper bound theorem to examine the system's failure mechanism and critical yield acceleration coefficient involving a retaining wall, ropes, and soil. This framework identified two distinct failure modes: the long-heel baseplate failure mode and the short-heel baseplate failure mode. These failure modes were distinguished based on the second and third conditions for generating slip surfaces in the analysis. Assuming that the sliding surface of the backfill follows a straight line, this study applies the Mohr-Coulomb failure criterion to establish critical state equations of pre-stressed ropes in a retaining wall with a heel, considering two distinct failure modes. Seismic yield acceleration coefficients are derived for each failure mode. Utilising the extremum principle, the study offers an optimal solution, including determining the critical yield acceleration coefficient and the associated inclination angle of the backfill sliding surface.
Mitochondrial Transfer/Transplant in Skin: Reducing ROS Damage, Enhancing Proliferation and Wound Healing
Authors: Peñaherrera Sebastian , Robayo Paola , Villagomez Andrés , Borja Tatiana , Arteaga María Belén , Benavides-Almeida Abigail , Pérez-Meza Álvaro , Haro-Vinueza Alissen , Segnini Gilberto , Pontón Patricia , Torres Fernando , Donoso Gustavo , Suquillo Daniela , Santos Lucas Ferreira dos , Arizo Pamela , Tenesaca Domenica , Antilef Barba , Zabala Gabriela , Barba Diego , Aponte Pedro , Cabrera Francisco , Luz Patricia , Moya Ivan , Mitrani María Ines , Khoury Maroun , Nova-Lamperti Estefanía , Burzio Verónica , Díaz Ramiro , Maron-Guitierrez Tatiana , Caicedo Andrés 
Published: October 2023

Abstract

The intricate cellular composition of the skin encompasses dynamic interactions among melanocytes, keratinocytes, and fibroblasts, crucial for protective responses to ultraviolet radiation (UVR), wound healing and aging. Recent insights underline mitochondrial transfer as pivotal in cellular repair, yet its occurrence between skin cells remains unclear. Our research probes mitochondrial exchanges between skin cells, especially post-UVR exposure, uncovering a predominance of transfer via tunneling nanotubes (TNTs) over microvesicles (MVs). Notably, fibroblasts show diminished mitochondrial acquisition, possibly accelerating aging and reactive oxygen species (ROS)-induced damage. To counteract this, we subjected fibroblasts to UVR, augmenting ROS production, and then initiated Artificial Mitochondrial Transfer/Transplant (AMT/T) using Mesenchymal stem cells (MSCs) as a source. This intervention markedly boosted fibroblast proliferation, countering the adverse effects of UVR-related stress reflected in reduced proliferation and escalated ROS levels. Investigating AMT/T's therapeutic potential, we applied MSC-sourced mitochondria to a mouse surgical wound model, resulting in a faster wound healing with minimal mitochondria (25 ng) and MSCs (250,000) introduced. These results emphasize mitochondrial transfer's critical role in skin cellular defense mechanisms, presenting a novel strategy for mitigating UVR-induced damage, enhancing wound recovery, and the potential shielding of skin from environmental stressors.
Pharmacokinetics and Tissue Distribution of Isovitexin-2''-O-β-D-glucopyranoside
(IVG) in Sprague-Dawley Rats
Authors: Zhao Xiaotong , Fang Leilei , Yang Tan , Zhang Yanqing , Xie Junbo 
Published: October 2023

Background::
Isovitexin-2"-O-D-glucopyranoside (IVG) has been known to exhibit sedative and hypnotic
effects. However, there is little understanding of the in vivo pharmacokinetics and tissue distribution of IVG.

Objective::
This study aimed to investigate the pharmacokinetics and tissue distribution of IVG.

Methods::
The study employed an HPLC–ESI-MS/MS method to analyze the pharmacokinetics and tissue distribution
of IVG.

Results::
Under mass spectrometry, IVG and internal standard (IS) showed strong negative ionization signals.
MRM analysis chose ion transitions m/z 593.3 → 293.0 (IVG) and m/z 579.8 → 271.4 (IS). Method validation
indicated high precision, accuracy, and reliability with a quantitation limit under 20 ng/mL. After intravenously
administering 5.0 mg/kg of IVG, rapid clearance from rat plasma was observed, with a half-life (t1/2) of 3.49 ±
0.99 h and a clearance rate of 54.53 ± 11.90 mL/kg/h. The area under the curve (AUC0-12h) of 37.79 ± 7.65
μg·h/mL indicated a brisk metabolic rate. Evaluating the tissue distribution, the highest accumulation was seen in
the liver (30.32 ± 3.06 μg/g), followed by the kidney (20.58 ± 2.12 μg/g) and intestine (6.69 ± 0.93 μg/g), suggesting
a propensity for IVG to concentrate in these tissues. Importantly, the presence of IVG in the brain underlines
its potential to traverse the blood-brain barrier. These findings revealed that following intravenous administration,
IVG was swiftly and broadly distributed throughout various rat tissues.

Conclusion::
This study provides valuable information on the pharmacokinetics and tissue distribution of IVG,
implicating its potential as a novel and effective drug candidate for sedative and anxiolytic treatment.

other:
none
RNA Sequencing of A2780 Cells Treated with CCL2 Identified Genes
Associated with A2780 Cell Growth
Authors: Ma Zhenling , Wang Lei , Cheng Kun , Xing Guozhen , Zhang Jiajia , Liu Wei 
Published: October 2023

Introduction:
Ovarian cancer is a common gynecological malignancy. It is one of the
leading causes of death among women worldwide. The incidence of ovarian cancer ranks third, and
mortality is the first among gynecological malignant tumors. CCL2 (Chemokine C-C motif Ligand
2) is associated with the progression of a variety of tumors, including ovarian cancer. However, the
mechanism of CCL2 in A2780 cell growth has not been clarified. Method: In this study, we found
that exogenous CCL2 promoted A2780 cell activity. RNA sequencing was used to identify the transcriptomic
changes in CCL2-treated A2780 cells. Based on a p-value less than 0.05 and |log2 Fold
Change| greater than 1, 190 differentially expressed genes were selected. Of these genes, 82 were
observed to be upregulated and 108 downregulated. Result: The GO (gene ontology) analysis of differentially
expressed genes was used to identify the underlying functions and biological processes.
In addition, the expression of the topmost upregulated genes was verified by qPCR. Conclusion:
This work may provide new markers and reveal the underlying mechanism of exogenous CCL2 in
A2780 cell proliferation.
The Diagnostic Value of a Nomogram Based on Clinical Imaging and MRIBased
Radiomic Features in Triple-Negative Breast Cancer
Authors: Meng Xin Liu , Min Ge , Shi Wei Wang , Huan Lu , Zhi Yong Pan , Xue Wei Ding 
Published: October 2023

Objective::
This study aimed to determine the utility of a radiomic nomogram combined with clinical imaging and radiomic features based on MRI for the
diagnosis of triple-negative breast cancer.

Methods::
Multi-parametric MRI images of 136 breast cancer patients were retrospectively analyzed, 95 cases were stratified into the training cohort, and 41
cases were selected for the test group. According to the pathological molecular typing, the patients were divided into 23 cases of triple-negative
breast cancer and 113 cases of non-triple-negative breast cancer. ITK software was used to manually delineate the lesion volume region of interest
(VOI), and the Pyradiomics package was used to extract radiomic features for screening and model building. The platform was then used to
analyze the clinical and imaging risk factors of breast cancer to build a characteristic model separately. Finally, a radiomic nomogram was
constructed by integrating the radiomic and independent clinical image features. The diagnostic performance of the model was assessed using ROC
curves

Results::
Univariate and multivariate analyses showed that the menstrual cycle, glandular density, and skin thickening were risk factors for clinical imaging
characteristics of triple-negative breast cancer. The Area Under the Curve (AUC) was 0.839 and 0.826 for univariate and multivariate analysis,
respectively. After screening, 11 radiomic features participated in the calculation of the radiomic score, and its AUC in the test set was 0.803.
Combining it further with clinical models, the AUC improved to 0.899.

Conclusion::
The radiomic nomogram developed in this study has great value in the diagnosis of triple-negative breast cancer.