bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Vitamin B1 Application on The Growth of Cocoa Seedlings
Authors: Harahap Fitri Sauda , Novalina Novalina 
Published: January 2025

Vitamin B1 is a vitamin that has a very important role for plants. The application of vitamin B1 (thiamin) is expected to increase plant growth, including in the seedling phase. This study aims to determine the effect of vitamin B1 application, variety and interaction of vitamin B1 and variety on the growth of cocoa seedlings. The research was conducted in the gauze house. Faculty of Agriculture, Universitas Sumatra Utara Medan, from July to December 2023. This study used a factorial randomized block design (RBD) consisting of 2 treatment factors. The first factor was cocoa plant variety consisting of three levels namely ICS 60, RCC 70, Lindak, and the second factor was vitamin B1 application (concentration of 10 ml/liter) consisting of 4 application method namely control, soaking seeds, foliar aplication and soaking seed + foliar application. Observation variables were plant height, stem diameter, number of leaves, leaf area. The results showed that vitamin B1 application, variety, interaction between varicty and vitamin B1 did not significantly affect all observation variables except vitamin B1 application significantly affected stem diameter of 6 week old cocoa seedlings. However, based on several observation variables, it is known that seedlings of Lindak variety which sprayed vitamin B1 (V3B2) had better growth than other treatment combinations.
THE EFFECT OF USING DAILY CONVERSATION YOUTUBE VIDEO TOWARDS STUDENT SPEAKING SKILL AT THE ELEVENTH GRADE STUDENT OF SMAN 1 CIHARA, LEBAK BANTEN IN ACADEMIC YEAR 2021/2022
Authors: Wini Wini nuraini , Ade Sudirman, M.Pd  , Rizdki Elang Gumelar, M.Pd  
Published: January 2025

Wini Nuraini (2022). “The effect of using daily conversation YouTube video towards student speaking skill at the eleventh grade student of SMAN 1 Cihara, Leabak Banten, Academic Year 2021/2022”.
This research paper aims to determine the significant effect of daily conversation YouTube videos on speaking skill of Class Xl SMAN 1 students Cihara Lebak-Banten Academic Year 2021/2022. Research Paper using a quantitative approach. This research is conducted from October to November, and researcher have chosen two classes, namely experimental class and control class. The technique in selecting the sample is take random cluster sampling; And analyzing the data, the researcher used Paired sample test with SPSS 22 statistics to analyze the hypothesis test. Researcher have found t-count results (-5.971) and Significant = 0.000 <0.05. From this the data can be interpreted that there is a significant difference between the two group in obtaining a score, because the result is a significance value of less than 0.05 which means Ha is accepted. Therefore, the researcher concludes that. The application of daily conversation YouTube videos has a good effect on students' speaking skills.
Keywords: daily conversation, YouTube video, speaking skill.
Pendampingan Peer Edukator Suami dalam Upaya Mendukung Keberhasilan ASI Eksklusif di Puskesmas Hutaimbaru Kota Padangsidimpuan
Authors: Batubara Novita Sari , Heriansyah Rizka , Lubis Tapi Endang Fauziah 
Published: January 2025

Pemberian ASI (Air Susu Ibu) merupakan nutrisi yang dibutuhkan untuk pertumbuhan dan perkembangan bayi. Bayi berusia 0-6 bulan haruslah diberikan hanya ASI saja, informasi terkait cakupan ASI eksklusif didapati sangat rendah. Beberapa data menyebutkan hal ini diakibatkan oleh kurangnya dukungan orang terdekat mengenai pemberian ASI eksklusif. Dukungan orang sekitar atau orang terdekat khususnya suami menjadi salah satu faktor penentu keberhasilan menyusui. Pemberdayaan keluarga melalui peer edukator suami sangat dibutuhkan untuk membantu keberhasilan pemberian ASI ekslusif. Sehingga dibutuhkan kegiatan pendampingan peer educator suami dengan memberikan penyuluhan, pelatihan mengenai ASI eksklusif. Metode pendampingan yang dilakukan yaitu metode partisipatif dengan melakukan pendekatan terhadap suami serta tokoh masyarakat setempat untuk mendapatkan perizinan dan dukungan dalam melakukan program pemberdayaan keluarga melalui peer eduk ator suami dalam upaya mendukung keberhasilan pemberian ASI Eksklusif di wilayah binaan. Jenis kegiatan ini berupa serangkaian kegiatan pendampingan berupa penyuluhan dan pemberian edukasi kepada suami untuk menambah pengetahuan dalam memberikan dukungan untuk keberhasilan pemberian ASI Eksklusif. Tempat atau lokasi kegiatan pengabdian masyarakat dilaksanakan di Puskesmas Hutaimbaru Kota Padangsidimpuan dengan kelompok sasaran adalah warga lingkungan sekitar. Hasil kegiatan pendampingan berdampak baik khusunya bagi para suami atau keluarga dimana suami dapat mengetahui manfaat, cara pemberian yang diharapkan dapat memberikan dukungan sepenuhnya dalam pemberian ASI Eksklusif
Sex-specific differences in the efficacy of renal denervation in patients with resistant hypertension depending on visceral obesity and kidney function
Authors: Zyubanova Irina , Ryumshina Nadezhda , Mordovin Victor , Manukyan Musheg , Lichikaki Valeriya , Solonskaya Ekaterina , Gusakova Anna , Suslova Tatjana , Pekarskiy Stanislav , Khunkhinova Simzhit , Popova Anastasia , Rudenko Veronika , Falkovskaya Alla 
Published: January 2025

ObjectiveTo investigate the sex differences in the efficacy of renal denervation (RDN) in patients with resistant hypertension (RHT) concerning the size of abdominal fat depots, changes in biomarkers of sympathetic activity, and renal function.Materials and methods24 men (56.5 ± 7.8 years) and 33 women (59.5 ± 8.4 years) with RHT were enrolled in the study and underwent RDN. 24-h ambulatory blood pressure (BP) [systolic/diastolic (SBP/DBP)], serum creatinine (with eGFR calculation), serum adipocytokines (leptin, adiponectin, resistin), serum metanephrines and normetanephrines were measured baseline and 12 months after RDN. The size of subcutaneous, visceral, and perirenal adipose tissue (SAT, VAT, and PRAT) was assessed using MRI.ResultsAfter RDN, BP decreased, leptin increased, and adiponectin, resistin, and metanephrine levels did not change in both sexes. There was a decrease in normetanephrine levels in women and a similar trend in men. In men, the eGFR did not change. In women, the eGFR remained unchanged only in those with chronic kidney disease (CKD) (n = 10) and decreased in the absence of CKD (n = 23) from 79.7 ± 14.1 to 72.1 ± 12.0 ml/min/1.73 m2 (p = 0.011). Men had larger visceral fat depots, and women had larger subcutaneous fat depots. After RDN, the size of adipose tissue in men remained unchanged, and in women, the PRAT thickness decreased from 2.36 ± 1.23 to 2.10 ± 1.17 cm (p = 0.002). Lowering BP in women was associated with increased leptin levels after RDN (r = −0.47 for SBP, r = −0.48 for DBP). Dependence of BP reduction on baseline eGFR was observed in men only [r = 0.44 for SBP, r = 0.48 for pulse pressure (PP)]. Additionally, in men, the decrease in SBP and PP depended on VAT areas (r = −0.44 and r = −0.58, respectively). In women, the SBP reduction showed an inverse correlation between baseline weight (r = −0.35) and waist circumference (r = −0.38).ConclusionsThe magnitude of the antihypertensive effect of RDN depends on signs of visceral obesity and, in men, also on the presence of CKD. Renoprotective effects of RDN in men are obtained regardless of the initial kidney function, while in women, it was observed only in individuals with CKD. Additional beneficial effects of RDN in women include a decrease in normetanephrine levels and a reduction in PRAT size.
Influencing factors of acute kidney injury in elderly patients with diabetic nephropathy and establishment of nomogram model
Authors: Wu Ganlin , Ye Yanli , Xu Meirong , Zhang Yanxia , Lu Zuopeng , Huang Lv 
Published: January 2025

PurposeTo explore the influencing factors of acute kidney injury in elderly patients with diabetic nephropathy and to construct a nomogram model.MethodsThe research subjects were 680 patients with type 2 diabetic nephropathy admitted to our hospital. The patients were included from May 2018 to August 2023. Patients with acute kidney injury were used as the merge group (n=50), and patients without unmerge group (n=630) was included. The prevalence and predisposing factors of acute kidney injury in diabetic nephropathy were analyzed, multivariate logistic regression were used to analyze the influencing factors of acute kidney injury in patients, and a nomogram risk prediction model was established based on risk factors for verification.ResultsAnalysis of the factors of acute kidney injury in diabetic nephropathy found that severe infection was the main trigger, accounting for 40.00%, followed by nephrotoxic antibiotics and severe heart failure. The age, urine microalbumin-to-creatinine ratio (ACR), blood urea nitrogen (BUN), uric acid(UA), and cystatin C (CysC) levels of patients in the combined acute kidney injury group were significantly higher than those in the unmerge group (P&lt;0.05), and the left ventricular ejection fraction (LVEF) and epidermal growth factor receptor (eGFR) levels were significantly lower than those in the unmerge group (P&lt;0.05). Age, ACR, and CysC levels are independent risk factors for acute kidney injury in diabetic nephropathy, and LVEF and eGFR are independent protective factors (P&lt;0.05). The C-index of the nomogram risk prediction model in predicting acute kidney injury in diabetic nephropathy is 0.768 (95% CI: 0.663-0.806), and the calibration curve tends to the ideal curve; the prediction threshold is &gt;0.18, and the nomogram risk prediction model provides a clinical net benefits, and clinical net benefits were higher than independent predictors.ConclusionThe establishment of a nomogram model for acute kidney injury in elderly patients with diabetic nephropathy based on age, ACR, CysC, LVEF, and eGFR has a good predictive effect, which can help doctors more accurately assess the patient’s condition and provide a basis for formulating personalized treatment plans.
Identification of a novel SPTB gene splicing mutation in hereditary spherocytosis: a case report and diagnostic insights
Authors: Li Xiaobing , Zhang Tingqiang , Li Xuemei , Wang Li , Li Qian , Liu Qianqian , He Chengyin , Zhang Li , Liu Yongsheng , Tang Junling 
Published: January 2025

BackgroundHereditary spherocytosis (HS) is a group of genetically heterogeneous hereditary hemolytic disorders characterized by anemia, splenomegaly, jaundice, reticulocytosis, and spherical red blood cells on peripheral blood smears. Mutations in key genes, including SPTB, ANK1, SLC4A1, SPTA1, and EPB42, are commonly implicated in HS.Case PresentationWe report the case of a 22-year-old female presenting with anemia, jaundice, and a family history of splenectomy. Laboratory investigations revealed hemolytic anemia, elevated bilirubin levels, and peripheral blood smear findings consistent with HS. Genetic testing identified a novel SPTB gene splicing mutation (NM_001355436.2: c.1645-1G&gt;A), inherited maternally, which is predicted to disrupt normal RNA splicing and protein synthesis.DiscussionThe identified SPTB mutation expands the known mutation spectrum of the SPTB gene and highlights its role in the pathogenesis of HS. Clinical findings, combined with genetic analysis, confirmed the diagnosis of HS and underscored the importance of comprehensive molecular testing for accurate diagnosis, especially in patients with a strong family history.ConclusionThis case emphasizes the utility of genetic testing in diagnosing hereditary spherocytosis, particularly for novel gene mutations. Early and accurate molecular diagnosis facilitates better clinical management, family counseling, and treatment decisions for patients with HS.
Development and validation of a nomogram for predicting immune-mediated colitis in lung cancer patients treated with immune checkpoint inhibitors: a retrospective cohort study in China
Authors: Xu Qianjie , Li Xiaosheng , Yuan Yuliang , Liang Guangzhong , Hu Zuhai , Zhang Wei , Wang Ying , Lei Haike 
Published: January 2025

BackgroundThe increasing utilization of immune checkpoint inhibitors (ICIs) has led to a concomitant rise in the incidence of immune-related adverse events (irAEs), notably immune-mediated colitis (IMC). This study aimed to identify the clinical risk factors associated with IMC development in patients with lung cancer and to develop a risk prediction model to facilitate personalized treatment and care strategies.MethodsThe data collected included 21 variables, including sociodemographic characteristics, cancer-related factors, and routine blood markers. The dataset was randomly partitioned into a training set (70%) and a validation set (30%). Univariate and multivariate logistic regression analyses were conducted to identify independent predictors of IMC development. On the basis of the results of the multivariate analysis, a nomogram prediction model was developed. Model performance was assessed via the area under the receiver operating characteristic curve (AUC), calibration curve analysis, decision curve analysis (DCA), and clinical impact curve (CIC).ResultsAmong the 2103 patients, 66 (3.14%) developed IMCs. Multivariate logistic regression analysis revealed female sex, small cell lung cancer (SCLC), elevated β2 microglobulin (β2-MG) and globulin (GLB) levels, and an increased neutrophil−lymphocyte ratio (NLR) as independent predictors of IMC development (all P &lt; 0.05). Conversely, a higher white blood cell (WBC) count, CD4/CD8 ratio, and platelet−lymphocyte ratio (PLR) were identified as factors associated with a reduced risk of IMC development (all P &lt; 0.05). The nomogram prediction model demonstrated good discrimination, achieving an AUC of 0.830 (95% CI: 0.774–0.887) in the training set and 0.827 (95% CI: 0.709–0.944) in the validation set. Analysis of the calibration curve, DCA, and CIC indicated good predictive accuracy and clinical utility of the developed model.ConclusionThis study identified eight independent predictors of IMC development in patients with lung cancer and subsequently developed a nomogram-based prediction model to assess IMC risk. Utilization of this model has the potential to assist clinicians in implementing appropriate preventive and therapeutic strategies, ultimately contributing to a reduction in the incidence of IMC among this patient population.
Chromatin accessibility and differentially expressed genes profiling in large yellow croaker (Larimichthys crocea) head kidney cells following iridovirus infection
Authors: Song Chaowei , Huang Ying , Han Fang , Wang Zhiyong 
Published: January 2025

IntroductionThe large yellow croaker iridovirus (LYCIV) poses a significant threat to the aquaculture industry of Larimichthys crocea. Understanding the host defense response to LYCIV infection is crucial for developing effective strategies to mitigate its impact.MethodsIn this study, an epigenetic approach was employed to investigate dynamic changes in chromatin accessibility using the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq). Additionally, RNA sequencing (RNA-seq) was used to analyze the expression pattern of immune response genes upon LYCIV infection.ResultsSubstantial alterations in chromatin accessibility were observed, particularly in the regulatory regions of key immune-related genes. Significant changes in the expression of AP-1 transcription factors, including the Batf gene, were noted. CUT&amp;Tag results revealed that AP-1 was significantly enriched in the open chromatin regions of cytokine genes, with Batf potentially regulating the cytokine genes LIF and CLCF1.DiscussionThese findings suggest that AP-1 may play a crucial role in the defense response against viral infection by modulating inflammatory cytokines and contributing to cellular inflammatory responses. This study provides a comprehensive analysis of the epigenomic landscape and gene expression regulation during iridovirus infection in L. crocea, offering valuable insights for breeding programs aimed at combating iridovirus infections.
Preoperative assessment of liver regeneration using T1 mapping and the functional liver imaging score derived from Gd-EOB-DTPA-enhanced magnetic resonance for patient with hepatocellular carcinoma after hepatectomy
Authors: Li Qian , Zhang Tong , Yao Shan , Gao Feifei , Nie Lisha , Tang Hehan , Song Bin , Wei Yi 
Published: January 2025

ObjectivesTo explore whether T1 mapping parameters and the functional liver imaging score (FLIS) based on Gd-EOB-DTPA MRI could evaluate liver regeneration after hepatectomy for HCC patient.MethodsThis retrospective study finally included 60 HCC patients (48 men and 12 women, with a median age of 53 years). T1 relaxation time of liver before gadoxetic acid injection (T1pre) and during the hepatobiliary phase (T1HBP), reduction rate (Δ%) and FLIS were calculated, their correlations with liver fibrosis stage, hepatic steatosis, and liver regeneration, quantified as regeneration index (RI), were assessed by Kendall’s tau-b correlation test or Spearman’s correlation test. Multivariate linear regression analyses were used to explore the indicator of RI.ResultsT1pre, T1HBP, Δ%, and FLIS manifested significant correlation with fibrosis stage (r = 0.434, P =0.001; r = 0.546, P &lt; 0.001; r = -0.356, P =0.005; r = -0.653, P &lt;0.001, respectively). T1pre showed significant correction with steatosis grade (r = 0.415, P =0.001). Fibrosis stage and steatosis grade were associated with RI (r = -0.436, P&lt;0.001; r = -0.338, P =0.008). Accordingly, T1pre, T1HBP and FLIS were the significant predictors (P&lt;0.05) of RI in multivariate analysis. Similarly, in the patients undergoing minor hepatectomy (n=35), T1HBP, Δ% and FLIS were related to RI (P&lt;0.05) in multivariate analysis. Nevertheless, in the patients undergoing major hepatectomy (n=25), no T1 mapping parameter and FLIS was the independent predictor of RI.ConclusionsT1 mapping parameters and FLIS were the potential noninvasive indicators of liver regeneration, except for HCC patients undergoing major hepatectomy.Clinical relevance statementThe value of T1 mapping and FLIS with Gd-EOB-DTPA MRI for accurate preoperative evaluation of liver regeneration is critical to prevent liver failure and improve prognosis of HCC patients.
Peripheral blood cell counts as predictors of immune-related adverse events in cancer patients receiving immune checkpoint inhibitors: a systematic review and meta-analysis
Authors: Zhang Xinyu , Zhang Bei , Li Danfei , Yang Yunchao , Lin Sen , Zhao Ruiqi , Li Yijia , Peng Lisheng 
Published: January 2025

BackgroundIn recent years, immune checkpoint inhibitors (ICIs) have shown significant efficacy in treating various malignancies and have become a key therapeutic approach in cancer treatment. However, while ICIs activate the immune system, they can also induce immune-related adverse events (irAEs). Due to the variability in the frequency and severity of irAEs, clinical management faces a significant challenge in balancing antitumor efficacy with the risk of irAEs. Predicting and preventing irAEs during the early stages of treatment has become a critical research focus in cancer immunotherapy. This study aims to evaluate the predictive value of peripheral blood cell counts for irAEs.MethodsStudies meeting the inclusion criteria were identified through database searches. The standardized mean difference (SMD) was used to compare continuous blood cell counts. For studies that did not provide adjusted odds ratios (ORs) and 95% confidence intervals (CIs), crude ORs for categorized blood cell counts were calculated. The study protocol was registered on PROSPERO (CRD42024592126).ResultsThe meta-analysis included 60 studies involving 16,736 cancer patients treated with ICIs. Compared to patients without irAEs, those experiencing irAEs had significantly higher baseline continuous ALC (SMD = 0.12, 95% CI = 0.01-0.24), while ANC (SMD = -0.18, 95% CI = -0.28 to -0.07) and PLR (SMD = -0.32, 95% CI = -0.60 to -0.04) were significantly lower. Similarly, categorized blood cell counts indicated that higher baseline ALC (OR = 2.46, 95% CI = 1.69-3.57) and AEC (OR = 2.05, 95% CI = 1.09-3.85), along with lower baseline NLR (OR = 0.64, 95% CI = 0.50-0.81) and PLR (OR = 0.63, 95% CI = 0.48-0.82), were associated with an increased risk of irAEs. Subgroup analysis further identified cutoff values for ALC (2×10^9/L), NLR (5 or 3), and PLR (180) as better predictors of irAEs.ConclusionHigher baseline ALC and AEC, along with lower baseline ANC, NLR, and PLR, are associated with an increased risk of irAEs. However, further research is needed to determine the optimal cutoff values and to explore the efficacy of blood cell counts in predicting specific types of irAEs.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024592126.
Supplementation with active vitamin D3 ameliorates experimental autoimmune thyroiditis in mice by modulating the differentiation and functionality of intrathyroidal T-cell subsets
Authors: Wang Chun-Mei , Chen Ying-Jie , Yang Bo-Cheng , Yang Jia-Wen , Wang Wei , Zeng Yang , Jiang Jun 
Published: January 2025

ObjectivePeople with Hashimoto’s thyroiditis (HT) often have low vitamin D3 concentrations. Some research has suggested that vitamin D3 supplementation reduces thyroid inflammation, but this remains controversial.MethodsEAT was induced in female NOD/ShiLtJ mice by giving them water containing 0.05% sodium iodide, and 1μg/kg of 1α,25-(OH)2D3 was injected intraperitoneally every other day. After 8 weeks, the morphological architecture of the mouse thyroid follicles was examined by histological sections, thyroid autoantibodies and thyroid hormone concentrations were determined by enzyme-linked immunosorbent assays (ELISAs), and the major functions and subsets of B- and T-lymphocytes in the mouse thyroid were determined by tissue multiple immunofluorescence technology and ELISA.ResultsEAT caused thyroiditis follicle destruction and interfollicular lymphocyte infiltration in mice, increased concentrations of circulating thyroid autoimmune antibodies TG-Ab and TPO-Ab, and abnormal thyroid hormone levels. EAT also increased the number and functionality of CD4+ Tfh, Th17,Th1 and Th2 cells in the thyroid, while decreasing the number and functionality of CD4+ Treg cells and CD19+B10 cells. Treatment with VD3 reversed these changes.ConclusionVitamin D3 supplementation can effectively treat autoimmune thyroiditis in mice. VD3 reduces autoimmune thyroid damage and decreases serum thyroid antibody levels in mice by inhibiting the differentiation and functionality of pro-inflammatory Tfh, Th17, Th1 and Th2 cells and by facilitating the differentiation and functionality of anti-inflammatory B10 cells and Treg.