bluuebunny/crossref_metadata_2025_split huggingface dataset:
S‐9‐PAHSA's neuroprotective effect mediated by CAIII suppresses apoptosis and oxidative stress in a mouse model of type 2 diabetes
Authors: Wang Jian‐tao , Wang Xin‐ru , Ren Jiao‐qi , Lin Jin‐hong , Yu Zhong‐yu , Huang Shan‐shan , Hu Yue , Fu Jia‐yu , Wang Meng , Zhang Yan‐li , Wang Xue‐chun , Guo Jing‐chun , Xiao Ji‐chang , Zhou Hou‐guang 
Published: February 2024

AbstractBackgroundWith the rapidly increasing prevalence of metabolic diseases such as type 2 diabetes mellitus (T2DM), neuronal complications associated with these diseases have resulted in significant burdens on healthcare systems. Meanwhile, effective therapies have remained insufficient. A novel fatty acid called S‐9‐PAHSA has been reported to provide metabolic benefits in T2DM by regulating glucose metabolism. However, whether S‐9‐PAHSA has a neuroprotective effect in mouse models of T2DM remains unclear.MethodsThis in vivo study in mice fed a high‐fat diet (HFD) for 5 months used fasting blood glucose, glucose tolerance, and insulin tolerance tests to examine the effect of S‐9‐PAHSA on glucose metabolism. The Morris water maze test was also used to assess the impact of S‐9‐PAHSA on cognition in the mice, while the neuroprotective effect of S‐9‐PAHSA was evaluated by measuring the expression of proteins related to apoptosis and oxidative stress. In addition, an in vitro study in PC12 cells assessed apoptosis, oxidative stress, and mitochondrial membrane potential with or without CAIII knockdown to determine the role of CAIII in the neuroprotective effect of S‐9‐PAHSA.ResultsS‐9‐PAHSA reduced fasting blood glucose levels significantly, increased insulin sensitivity in the HFD mice and also suppressed apoptosis and oxidative stress in the cortex of the mice and PC12 cells in a diabetic setting. By suppressing oxidative stress and apoptosis, S‐9‐PAHSA protected both neuronal cells and microvascular endothelial cells in in vivo and in vitro diabetic environments. Interestingly, this protective effect of S‐9‐PAHSA was reduced significantly when CAIII was knocked down in the PC12 cells, suggesting that CAIII has a major role in the neuroprotective effect of S‐9‐PAHSA. However, overexpression of CAIII did not significantly enhance the protective effect of S‐9‐PAHSA.ConclusionS‐9‐PAHSA mediated by CAIII has the potential to exert a neuroprotective effect by suppressing apoptosis and oxidative stress in neuronal cells exposed to diabetic conditions. Furthermore, S‐9‐PAHSA has the capability to reduce fasting blood glucose and LDL levels and enhance insulin sensitivity in mice fed with HFD.
HTRA1 promotes EMT through the HDAC6/Ac‐α‐tubulin pathway in human GBM cells
Authors: Zhao Wenbo , Wu Yibo , Wang Shuai , Zhao Feihu , Liu Wenyu , Xue Zhiyi , Zhang Lin , Wang Jian , Han Mingzhi , Li Xingang , Huang Bin 
Published: February 2024

AbstractBackgroundThe infiltrative nature of human gliomas renders complete surgical removal of tumors futile. Thus, illuminating mechanisms of their infiltrative properties may improve therapies and outcomes of glioma patients.MethodsComprehensive bioinformatic analyses of PRSS family were undertaken. Transfection of HTRA1 siRNAs was used to suppress HTRA1 expression. CCK‐8, EdU, and colony formation assay were employed to assess cell viability, and cell migration/invasion was detected by transwell, wound healing, and 3D tumor spheroid invasion assays. Immunoprecipitation was applied to study the mechanism that HTRA1 affected cell migration. In addition, in situ xenograft tumor model was employed to explore the role of HTRA1 in glioma growth in vivo.ResultsHTRA1 knockdown could lead to suppression of cell viability, migration and invasion, as well as increased apoptosis. Immunoprecipitation results indicates HTRA1 might facilitate combination between HDAC6 and α‐tubulin to enhance cell migration by decreasing α‐tubulin acetylation. Besides, HTRA1 knockdown inhibited the growth of xenografts derived from orthotopic implantation of GBM cells and prolonged the survival time of tumor‐bearing mice.ConclusionOur results indicate that HTRA1 promotes the proliferation and migration of GBM cells in vitro and in vivo, and thus may be a potential target for treatment in gliomas.
Protein tyrosine phosphatase 1B contributes to neuropathic pain by aggravating NF‐κB and glial cells activation‐mediated neuroinflammation via promoting endoplasmic reticulum stress
Authors: Jiao Bo , Zhang Wencui , Zhang Caixia , Zhang Kaiwen , Cao Xueqin , Yu Shangchen , Zhang Xianwei 
Published: February 2024

AbstractBackgroundNeuropathic pain is a prevalent and highly debilitating condition that impacts millions of individuals globally. Neuroinflammation is considered a key factor in the development of neuropathic pain. Accumulating evidence suggests that protein tyrosine phosphatase 1B (PTP1B) plays a crucial role in regulating neuroinflammation. Nevertheless, the specific involvement of PTP1B in neuropathic pain remains largely unknown. This study aims to examine the impact of PTP1B on neuropathic pain and unravel the underlying molecular mechanisms implicated.MethodsIn the current study, we evaluated the paw withdrawal threshold (PWT) of male rats following spared nerve injury (SNI) to assess the presence of neuropathic pain. To elucidate the underlying mechanisms, western blotting, immunofluorescence, and electron microscopy techniques were employed.ResultsOur results showed that SNI significantly elevated PTP1B levels, which was accompanied by an increase in the expression of endoplasmic reticulum (ER) stress markers (BIP, p‐PERK, p‐IRE1α, and ATF6) and phosphorylated NF‐κB in the spinal dorsal horn. SNI‐induced mechanical allodynia was impaired by the treatment of intrathecal injection of PTP1B siRNA or PTP1B‐IN‐1, a specific inhibitor of PTP1B. Moreover, the intrathecal administration of PTP1B‐IN‐1 effectively suppressed the expression of ER stress markers (BIP, p‐PERK/p‐eIF2α, p‐IRE1α, and ATF6), leading to the inhibition of NF‐κB, microglia, and astrocytes activation, as well as a decrease in pro‐inflammatory cytokines, including TNF‐α, IL‐6, and IL‐1β. However, these effects were reversed by intrathecal administration of tunicamycin (Tm, an inducer of ER stress). Additionally, intrathecal administration of Tm in healthy rats resulted in the development of mechanical allodynia and the activation of NF‐κB‐mediated neuroinflammatory signaling.ConclusionsThe upregulation of PTP1B induced by SNI facilitates the activation of NF‐κB and glial cells via ER stress in the spinal dorsal horn. This, in turn, leads to an increase in the production of pro‐inflammatory cytokines, thereby contributing to the development and maintenance of neuropathic pain. Therefore, targeting PTP1B could be a promising therapeutic strategy for the treatment of neuropathic pain.
Electroacupuncture ameliorates neuroinflammation by inhibiting TRPV4 channel in ischemic stroke
Authors: Ren Xueqi , Gao Xinyi , Li Ziqing , Ding Yangyang , Xu Ao , Du Lixia , Yang Yufang , Wang Deheng , Wang Zhifei , Shu Shi 
Published: February 2024

AbstractAimsWe investigated the potential mechanisms underlying the therapeutic efficacy of electroacupuncture (EA) at the Shuigou (GV26) and Baihui (GV20) acupoints in the treatment of ischemic stroke.MethodsWe assessed the therapeutic effects of EA on MCAO mice through behavioral studies and TTC staining. Various techniques, such as RT‐PCR, immunofluorescence, and Western blots, were employed to evaluate the activation and polarization of microglia/macrophages, and changes in the TRPV4 ion channel. We used the TRPV4 antagonist GSK2193874 (GSK219) to verify the involvement of TRPV4 in the therapeutic effects of EA.ResultsEA effectively improved neurological impairments and reduced cerebral infarction volume in MCAO mice. It suppressed activated microglia/macrophages and inhibited their polarization toward the M1 phenotype post‐MCAO. EA also downregulated the expression of pro‐inflammatory cytokines, including Tnf‐α, Il‐6, Il‐1β, and Ccl‐2 mRNA. Furthermore, EA reduced the elevated expression of TRPV4 following MCAO. Treatment with the TRPV4 antagonist GSK219 mirrored the effects of EA in MCAO mice. Notably, the combination of EA and GSK219 did not demonstrate an additive or synergistic effect.ConclusionEA may inhibit neuroinflammation and exhibit a protective effect against ischemic brain injury by suppressing TRPV4 and the subsequent M1 polarization of microglia/macrophages.
The alleviative effects comparison of four flavonoids from bamboo leaves on ulcerative colitis in an Alzheimer mouse model
Authors: Zhang Taiyu , Jia Cuicui , Ran Longyi , Shi Jiarui , Amarmend Tsendsuren , Li Huiying 
Published: February 2024

AbstractBackgroundClinically, patients with dementia are at high risk of developing enteritis, especially those with AD. This study explored the potential therapeutic benefits of bamboo leaf flavonoids (BLF) for ulcerative colitis (UC) treatment in Alzheimer's disease (AD) mouse model.MethodsVarious methods were employed, including pathological staining of brain/colon tissue, inflammatory cytokine detection in serum, and oxidative stress indicator assessment to compare ulcerative enteritis (UC) injury in normal and AD mice and determine whether AD mice were susceptible to colitis. Then, the effects of BLF on UC and AD were investigated via several unique indices further to determine whether it alleviated colitis injury and possessed beneficial properties. Moreover, four main components of BLF were utilized to treat primary colon epithelial cells and neuron cells to compare their effects in alleviating inflammation and oxidation. Furthermore, homoorientin embedded with ursolic acid was detected by HPLC and the in vitro release simulation experiments of the nanoparticles were performed.ResultsBLF complexes positively impacted ulcerative colitis by reducing disease activity, it also helped to reduce inflammation. Moreover, the BLF complexes decreased oxidative stress in the brain and colon tissues, indicating its potential as a neuroprotective agent. The flavonoid complexes reduced the expression levels of GFAP, Iba‐1, and Aβ in the brain tissue, highlighting its role in attenuating neuroinflammation and AD pathology. Additionally, the embedded homoorientin coated with ursolic acid showed stronger bioactivities when compared with the uncoated group.ConclusionThese results suggest that BLF complexes and its four main chemicals may be useful for treating AD‐ and UC‐related complications, the embedded homoorientin coated with ursolic acid even demonstrated stronger bioavailability than homoorientin. Considering BLF complexes were verified to suppress the progressions of AD and UC for the first time, and the embedded homoorientin was never reported in published articles, the present study might provide a new perspective on its potential applications.
ACTION APAC: Understanding perceptions, attitudes and behaviours in obesity and its management across south and Southeast Asia
Authors: Tham Kwang Wei , Ahmed Asma , Boonyavarakul Apussanee , Garcia Mariana Mercado , Guajardo Miroslava , Hanipah Zubaidah Nor , Nam Tran Quang , Nicodemus Nemencio A. , Pathan Faruque , Romano Jack Garcia Uranga , Soegonda Sidartawan , Tolentino Edgardo L. , Unnikrishnan Ambika Gopalakrishnan (A. G). , Oldfield Brian J. 
Published: February 2024

SummaryTo identify perceptions and attitudes among people with obesity (PwO) and healthcare professionals (HCPs) toward obesity and its management in nine Asia‐Pacific (APAC) countries, a cross‐sectional online survey was conducted among adult PwO with self‐reported body mass index of ≥25 kg/m2 (≥27 kg/m2, Singapore), and HCPs involved in direct patient care. In total, 10 429 PwO and 1901 HCPs completed the survey. Most PwO (68%) and HCPs (84%) agreed that obesity is a disease; however, a significant proportion of PwO (63%) and HCPs (41%) believed weight loss was the complete responsibility of PwO and only 43% of PwO discussed weight with an HCP in the prior 5 years. Most respondents acknowledged that weight loss would be extremely beneficial to PwO's overall health (PwO 76%, HCPs 85%), although nearly half (45%) of PwO misperceived themselves as overweight or of normal weight. Obesity was perceived by PwO (58%) and HCPs (53%) to negatively impact PwO forming romantic relationships. HCPs cited PwOs' lack of interest (41%) and poor motivation (37%) to lose weight as top reasons for not discussing weight. Most PwO (65%) preferred lifestyle changes over medications to lose weight. PwO and HCPs agreed that lack of exercise and unhealthy eating habits were the major barriers to weight loss. Our data highlights a discordance between the understanding of obesity as a disease and the actual behaviour and preferred approaches to manage it among PwO and HCPs. The study addresses a need to align these gaps to deliver optimal care for PwO.
The challenge of assessing the effects of drifting fish aggregating devices on the behaviour and biology of tropical tuna
Authors: Dupaix Amaël , Ménard Frédéric , Filmalter John D. , Baidai Yannick , Bodin Nathalie , Capello Manuela , Chassot Emmanuel , Demarcq Hervé , Deneubourg Jean‐Louis , Fonteneau Alain , Forget Fabien , Forrestal Francesca , Gaertner Daniel , Hall Martin , Holland Kim N. , Itano David , Kaplan David Michael , Lopez Jon , Marsac Francis , Maufroy Alexandra , Moreno Gala , Muir Jeff A. , Murua Hilario , Roa‐Pascuali Liliana , Pérez Géraldine , Restrepo Victor , Robert Marianne , Schaefer Kurt M. , Sempo Grégory , Soria Marc , Dagorn Laurent 
Published: February 2024

AbstractFishers have intensively used drifting fish aggregating devices (DFADs) over the last three decades to facilitate their catch of tropical tunas. DFADs increase purse‐seine efficiency, potentially increasing tuna fishing mortality. They could also have impacts on tuna natural mortality and reproductive potential, and assessing the consequences of their presence at sea on tuna populations is a challenge. The use of DFADs results in a major increase in the number of floating objects, which are spatially heterogeneous at sea. To date, no converging scientific results exist regarding the effects of DFADs on the large‐scale movements and behaviour of tuna, mainly due to the difficulty of disentangling the respective roles of DFADs and environmental factors. Some biological indices show that tuna condition is lower when associated to a floating object than in a free‐swimming school. However, it is not clear whether this is the cause or the consequence of the association nor if it has long‐term effects on individuals' fitness. Further scientific progress requires (i) the collection of time series of indicators to monitor habitat change, individual behaviour, individual fitness, and population dynamics and (ii) experimental studies to identify the underlying behavioural and biological processes involved in associative behaviour. The extent of the modification of the surface habitat by the massive deployment of DFADs and the current uncertainty of the possible long‐term consequences on the individual fitness and dynamics of tuna populations argue for the need for increased awareness of this issue by Regional Fisheries Management Organisations regulating tuna fishing.
What determines mate choices? Heterospecific mating in Sympetrum dragonflies
Authors: Kornová Veronika , Bílková Eva , Pyszko Petr , Dolný Aleš , Ožana Stanislav 
Published: February 2024

Abstract

Heterospecific interactions are an important phenomenon of animal epigamic behaviour; however, they remain understudied within important groups, such as insects. Dragonflies are an ideal group for research on interspecific mating and possible hybridisation as they are easily identifiable, large insects, with a conspicuous mating process, often striking territorial behaviour and several mating systems.

Using the genus Sympetrum as the model group, we examined heterospecific mating at three different levels. In the field, we identified whether species identity, time and weather affected heterospecific mating frequency. One important part of heterospecific mating is whether the process is completed. For dragonflies, this means that flying in tandem is followed by successful copulation, gamete fusion and oviposition (which comprise mating completeness). In a mesocosm experiment, we determined mating completeness (tandems, copulation, oviposition) of hetero‐ and homospecific pairs and the possible role of species density in heterospecific mating. In the laboratory, we compared the viability of the offspring from heterospecific pairs with different epigamous behaviour.

We found heterospecific mating to be a relatively common phenomenon unaffected by environmental variables, that was primarily influenced by species identity, temporal distribution and abundance of dragonfly species. Consequently, the presence of counterparts of other species is the main predictor of the frequency of heterospecific mating.

The probability of completed epigamic behaviour (copulation and subsequent oviposition) connected with gamete fusion is lower in heterospecific mating. Generally, based on our results we can assume that successful heterospecific mating (leading to gamete fusion) occurs in closely related species (e.g., Sympetrum striolatum and Sympetrum vulgatum). However, as pre‐copulatory barriers are not strongly developed in some dragonfly groups, less closely related species (e.g., Sympetrum sanguineum and S. striolatum) also may mate. This phenomenon requires further study as it may present a threat to the survival of some species in the context of changing environmental conditions, including climate change.
Native forbs interseeded into native grass pastures persist under grazing
Authors: Richwine Jonathan D. , Keyser Patrick D. , Zobel John M. , Walker Forbes R. 
Published: February 2024

AbstractIncorporating native forbs within native warm‐season grass (NWSG) pastures has the potential to benefit cattle, pollinators, and wildlife beyond that of NWSG monocultures. However, when grazing NWSG pastures, rotational stocking is recommended as opposed to continuous stocking. Therefore, to evaluate whether within‐season rest is needed for native pasture sustainability, two NWSG grazing experiments were conducted near Greeneville, TN, 2017–2020, to assess the persistence of native forbs when an 11‐species native forb blend was interseeded into established switchgrass (SG; Panicum virgatum L.) and big bluestem/indiangrass (BBIG; Andropogon gerardii Vitman and Sorghastrum nutans (L.) Nash) pastures. Each experiment was a completely randomized design with four replicates of each within‐season rest grazing treatment (no rest, early rest, middle rest, late rest, and no graze). Within‐season rest was not influential for NWSG tiller density or total forb plant density thus indicating persistence of forbs may not require rotational stocking. Purple prairie clover (Dalea purpurea Vent.) did not establish while Illinois bundleflower (Desmanthus illinoensis (Michx.) MacMill. ex B.L. Rob. & Fernald) was only observed flowering once despite having the greatest seeding rate among forbs. Based on establishment and flowering of the 11 species in the current mixture, interseeding a 6‐species polyculture of black‐eyed Susan (Rudbeckia hirta L.), dixie ticktrefoil (Desmodium tortuosum (Sw.) DC.), eastern purple coneflower (Echinacea purpurea (L.) Moench), lanceleaf coreopsis (Coreopsis lanceolata L.), Maximilian sunflower (Helianthus maximiliani Schrad.), and oxeye sunflower (Heliopsis helianthoides (L.) Sweet) could allow for plant biodiversity while offering floral resources for pollinators during the NWSG grazing season.
Helicobacter hepaticus and Helicobacter bilis in liver and biliary cancers from ATBC and PLCO
Authors: Murphy Gwen , Freedman Neal D. , Abnet Christian C. , Albanes Demetrius , Cross Amanda J. , Huang Wen‐Yi , Koshiol Jill , McGlynn Katherine , Parisi Dominick , Männistö Satu , Weinstein Stephanie J. , Waterboer Tim , Butt Julia 
Published: February 2024

AbstractBackgroundHelicobacter species (spp.) have been detected in human bile and hepatobiliary tissue Helicobacter spp. promote gallstone formation and hepatobiliary tumors in laboratory studies, though it remains unclear whether Helicobacter spp. contribute to these cancers in humans. We used a multiplex panel to assess whether seropositivity to Helicobacter (H.) hepaticus or H. bilis proteins was associated with the development of hepatobiliary cancers in the Finnish Alpha‐Tocopherol, Beta‐Carotene Cancer Prevention (ATBC) Study, and US‐based Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO).MethodsWe included 62 biliary and 121 liver cancers, and 190 age‐matched controls from ATBC and 74 biliary and 105 liver cancers, and 364 age‐ and sex‐matched controls from PLCO. Seropositivity to 14 H. hepaticus and H. bilis antigens was measured using a multiplex assay. Odds ratios (ORs) and 95% confidence intervals (CIs) were adjusted for major hepatobiliary cancer risk factors and Helicobacter pylori serostatus.ResultsSeropositivity to the H. bilis antigen, P167D, was associated with more than a twofold higher risk of liver cancer (OR: 2.38; 95% CI: 1.06, 5.36) and seropositivity to the H. hepaticus antigens HH0407 or HH1201, or H. bilis antigen, HRAG 01470 were associated with higher risk of biliary cancer (OR: 5.01; 95% CI: 1.53, 16.40; OR: 2.40; 95% CI: 1.00, 5.76; OR: 3.27; 95% CI: 1.14, 9.34, respectively) within PLCO. No associations for any of the H. hepaticus or H. bilis antigens were noted for liver or biliary cancers within ATBC.ConclusionsFurther investigations in cohort studies should examine the role of Helicobacter spp. in the etiology of liver and biliary cancers.
Beyond the asylum‐applications growth. The limits of the Spanish refugee reception program
Authors: Iglesias Juan , Bermejo Rut , Bazaga Isabel 
Published: February 2024

AbstractBased on two extended qualitative research projects conducted between 2017 and 2022, this paper analyses the refugee reception programme (RP) in Spain, which is managed both by the central state and some specialised social organisations. This cross‐sectoral RP presents notable and enduring problems, which have deepened since the increase in asylum applications during the so‐called European refugee crisis in 2015. This paper affirms that, although this increase in asylum seekers represents a serious challenge, the persistent shortcomings of the RP are better explained by a set of structural factors related to (1) the restrictive institutional model of asylum and immigration policy, (2) the lack of development of the RP, its dispersal policy and its social intervention design, (3) the lack of multilevel governance between the State and the municipalities and regional administration and (4) the current neoliberal and nativist policies.