bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia-induced DTL promotes the proliferation, metastasis, and sorafenib resistance of Hepatocellular Carcinoma through Notch Signaling Pathway
Authors: Gao Fei , Chen Zi-Xiong , Mu Mao-Yuan , Yang Guang , Qi Han , Fu Xiao-Bo , Wang Gui-Song , Jiang Wei-Wei , Huang Bi-Jun 
Published: January 2024

Abstract

Denticleless E3 ubiquitin protein ligase homolog (DTL), the substrate receptor of the CRL4A complex, plays a central role in genome stability. Even though the oncogenic function of DTL has been investigated in several cancers, its specific role in Hepatocellular Carcinoma (HCC) still needs further elucidation. Data from a clinical cohort (n = 209), RNA-sequencing, and public database (TCGA and GEO) were analyzed, indicating that DTL is closely related to patient prognosis and could serve as a promising prognostic indicator in HCC. Functionally, DTL promoted the proliferation, metastasis, and sorafenib resistance of HCC in vitro. In the orthotopic tumor transplantation and tail vein injection model, DTL promoted the growth and metastasis of HCC in vivo. Mechanically, we revealed for the first time that DTL was transcriptionally activated by hypoxia-inducible factor 1α (HIF-1α) under hypoxia and functioned as a downstream effector molecule of HIF-1α. DTL facilitates HCC cell proliferation, metastasis, and epithelial-mesenchymal transition through the Notch pathway. These results suggested that DTL may be a potential biomarker and therapeutic target for HCC.
METTL14/YTHDC1-mediated m6A modification in hippocampus improves pentylenetetrazol-induced acute seizures
Authors: Zhong Xiaolin , Chen Ling , Wang Yajuan , Liang Yue , Huang Yanmei , Chen Zuyao , Cao Wenyu , Liu Jianghua , Zu Xuyu 
Published: January 2024

Abstract
Epilepsy is a common neurological disorder which can cause significant morbidity and mortality. N6-methyladenosine (m6A), the most common chemical epigenetic modification among mRNA post-transcriptional modifications, implicated in various physiological and pathological processes, but its role in epilepsy is still unknown. Here, we provide strong evidences in support of an association of m6A and its regulatory proteins with epilepsy. Our results indicated that the level of m6A was declined in the hippocampus of pentylenetetrazol (PTZ)-induced seizure mice. Both the seizure-like behaviors and the excessive activation of hippocampal neuron were significantly mitigated after the administration of m6A agonist Betaine. Mechanically, we found both the hippocampal m6A methyltransferase METTL14 and recognition protein YTHDC1 were decreased in PTZ kindled mice, which might contribute to the reduced hippocampal m6A level. Additionally, hippocampal-specific over-expression of METTL14 or YTHDC1 by lentivirus injection could significantly ameliorate seizure-like behaviors and prevent the excessive activation of hippocampal neuron in epilepsy mice induced by PTZ injection, which might be due to the normalized hippocampal m6A level. Together, this study identified METTL14/YTHDC1-mediated m6A modification could participate in seizure-like behaviors, which might provide m6A regulation as a potential and novel therapeutic strategy for epilepsy.
Factors Associated with Stunting Among Children Aged 6 to 59 Months in Nyabihu District. A Case Control Study 2023.
Authors: MATSIKO Eric , HIRWA Emmerance IGIHOZO , MUKANYANGEZI Francoise 
Published: January 2024

Abstract
INTRODUCTION:
Malnutrition is a leading cause of mortality among children around the world, with over 6 million deaths occurring each year and an estimated 155 million worldwide being stunted in 2016. Rwanda has one the highest rates of child stunting in the world with 33%. Therefore, the study aimed to identify factors associated with stunting among 6 to 59 months’ children in Nyabihu district, Rwanda.
METHODS
A matched case-control study design was used to identify factors associated with stunting among children aged 6 to 59 months who attended health centers in Nyabihu district from March to April 2023.A total of 402 (134 cases and 268 control) children with 6 to 59 months of age were included in the study. Cases were selected based on WHO anthropometric measurements and matched with children of the same age and residence. Primary data on social demographic, hygiene and sanitation, feeding practices and clinical characteristics were collected using a structured questionnaire. The data were entered in Excel and exported in STATA for analysis. Both bivariate and multivariate logistic regression analysis were performed to identify associated factors. P values < 0.05 with a 95% confidence level were used to declare statistical significance.
RESULTS
The following factors were significant to child’s stunting: being in Ubudehe cat 1 (AOR 11.1; 95% CI 4.3–8.4), Non-parental caregivers (AOR 3.8; 95% CI 2-7.4) and having more than 2 under-five children (AOR 5; 95% CI 1.5–6.2), mostly Sell agriculture use (AOR 4.3; 95% CI 2.3–7.9), Not treating drinking water (AOR 2.4; 95% CI 1.3–4.5), Washing hands (AOR 0.08; 95% CI 0.04–0.1), Not attending ANC (OR 8; 95% CI 1.8– 4.7), Attending mass campaign (AOR 0.34; 95%CI 0.1–0.6).
CONCLUSION
Child stunting remains a public health problem in Nyabihu District. The findings show that the first Ubudehe category, washing hands before child feeding, treating drinking water, more number of under-five children in the household, attending ANC and vaccination campaigns were determinants of stunting. Relieving poverty, strengthening antenatal care, child vaccination and improvement in hygiene and sanitation can help to reduce the stunting rate.
Hidden in plain sight: Revisiting the synthesis, characterisation, degradation and the intricate relationship between Scheele’s green and Emerald green
Authors: Munoz Leonardo Pantoja 
Published: January 2024

Abstract
Carl Wilhelm Scheele's legacy is primarily associated with the creation of a notorious toxic pigment, commonly referred to as "Scheele's green." The identification of Scheele's green in comparison to Emerald green poses theoretical complexities. Common assumptions about the degradation of Emerald green suggest dissociation in acidic pH conditions, generating mobile arsenic trioxide and copper ions, followed by oxidation to As(V), which can co-precipitate with Fe, Al, and Ca ions.
In this study, the fabrication of Scheele’ green was reproduced using historical recipes, with a specific focus on maintaining the pH of the arsenite solution at 9.3. The study explores its relationship with Emerald green and the challenges associated with identification, aiming to inform heritage object preservation and enhance health and safety guidelines for pigment handling.
Maintaining the pH at 9.3 proved influential in obtaining a crystalline product with an intense Raman signal, aligning with the widely accepted spectra of Scheele’s green. However, Raman spectra from amorphous copper-arsenite samples consistently exhibit broad bands at 288 and 845 cm-1, prompting a proposed modification for a dual representation of the pigment: the “common” form with broad bands and the “uncommon” or crystalline form reported in the literature.
Demonstrating that the crystalline form shares nearly identical Raman and FTIR spectra implies an identical chemical composition to Trippkeite. Evidence presented highlights that copper-arsenic-based pigments contain adsorbed free copper, arsenite, and arsenate ions prone to migration, challenging commonly described degradation pathways.
The hypothesis presented here, that Emerald green synthesis may inadvertently yield small amounts of Scheele’s green urges caution in pigment identification using Raman spectroscopy. Additionally, the study reveals, for the first time, the occurrence of Scheele’s green in a book, with particles exhibiting a spherulite form, challenging identification of Emerald green solely based on morphology.
Legal and Political Feasibility of Implementing Front-of-Pack Labelling Interventions to Prevent Nutrition-Related Non-Communicable Diseases in Kenya
Authors: Ojiambo Veronica Sanda , Kruger Petronell , Wanjohi Milka , Mafuyeka Mikateko , Karugu Caroline , Mugo Sharon , Hoffman Karen , Asiki Gershim 
Published: January 2024

Abstract
Background: Kenya lags in the adaptation and implementation of front-of-pack labeling interventions despite the rising prevalence of nutrition-related non-communicable diseases. It is important to explore the factors influencing the political acceptability and legal feasibility of this policy recommendation to support its uptake in the Kenyan context. The objective of this study is to assess the legal and political feasibility of implementing front-of-pack labeling (FOPL) interventions for the prevention of nutrition-related non-communicable diseases in Kenya.
Design: The feasibility assessment used the novel FELIP Framework. We conducted desk-based reviews of the existing laws, laws related to the sector, the present legal infrastructure, and processes involved in adopting the laws to identify potential policy formulations. We also conducted key informant interviews with stakeholders on their views on the barriers, facilitators, and legal and political feasibility of the FOPL policy. The findings from the key informant interviews and desk reviews were analyzed thematically and mapped against the original policy formulations identified as part of the FELIP method.
Results: Policy and decision-makers in Kenya are in support of the adoption and implementation of a front-of-pack labeling intervention. The main facilitators to the development of the policy identified were (1) the availability of supporting evidence, (2) the development of standards, and (3) the identification of an appropriate policy anchor and administrative infrastructure. The main barriers were (1) nutrition literacy, and (2) industry interference.
Conclusion: The adoption and implementation of a front-of-pack labeling system in Kenya is largely politically acceptable and the legal infrastructure is favorable to anchor the policy. There is a need to increase political will in support of public health interests over commercial interests. Public health campaigns are essential to realize the effective uptake of the intervention at the population level.
Education, socioeconomic status, leisure sedentary behaviours and female infertility: Mendelian randomization study
Authors: qu yiyuan , huang xujia , mi jinqiao , chen xuzhi , wu bingbing , xu jian 
Published: January 2024

Abstract
Background: Growing evidence suggests that education, socioeconomic status, and leisure sedentary behaviors are associated with female infertility. However, it remains uncertain whether these connections are causally related.
Methods: Genetic variants from genome-wide association studies (GWAS) of education, socioeconomic status, and leisure sedentary behaviors were obtained from the UK Biobank and EBI database. Summarized GWAS data of female infertility were also acquired from the FinnGen Biobank Analysis. Univariable and multivariable MR analyses were performed to explore the relationships between education, socioeconomic status, leisure sedentary behaviors and female infertility. The primary analysis utilized inverse variance weighting, while alternative Mendelian Randomization (MR) methods such as MR-Egger, weighted median, and weighted mode estimate, along with Cochrane’s Q test, MR-Egger intercept test, MR pleiotropy residual sum and outliers (MR-PRESSO) test, and leave-one-out analysis were employed to evaluate sensitivity and heterogeneity.
Results: The results of the univariate MR analysis indicated that age of full- time education had a protective effect on female infertility (odds ratio [OR] 0.471; 95% confidence interval [CI] 0.24 to 0.93; p = 0.03). However, no causal correlation was found between educational attainment (college completion and years of education), socioeconomic status (job involving heavy manual or physical work, average total household income before tax, and Townsend deprivation index at recruitment), leisure sedentary behaviors (leisure social activities at pubs or social clubs, time spent driving, time spent using a computer, and time spent watching television) and female infertility. Multivariable MR and reverse MR studies did not support the existence of a relationship between education, socioeconomic status, leisure sedentary behaviours and female infertility. No significant heterogeneity or horizontal pleiotropy was detected, and the stability of the results was confirmed through sensitivity analysis and the leave-one-out test.
Conclusions: Age at completion of full-time education may be causally related to a reduced risk of female infertility.
Magnetic nanoparticles composed of Fe3(PO4) 2 ·8H2O by Burkholderia cepacia CG-1：A study of peroxidase-like activity and its application in H2O2 and glucose detection
Authors: Wang Jueyu , Jia Mingyu , Yang Jinming , Cui Daizong , Zhao Min 
Published: January 2024

Abstract
In this study, magnetic nanoparticles were successfully separated from the strain Burkholderia cepacia CG-1. The composition, structure and morphology of the magnetic nanoparticles were characterized. The results indicated that the magnetic nanoparticles were indicated as Fe3(PO4)2·8H2O clusters with an average size of 69 nm. The values of magnetic parameters such as coercivity, saturation remanence and saturation magnetization were 150.93 Oe, 1.9333 emu/g and 15.844 emu/g, respectively. We have confirmed that the Fe3(PO4)2·8H2O magnetic nanoparticles exhibitted an intrinsic peroxidase-like activity which could catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2. Comparing with HRP, the peroxidase mimic was more stable under different physiochemical conditions. The mechanisms of the peroxidase-like catalysis with Fe3(PO4)2·8H2O were investigated by the fluorescence spectroscopy and Electron spin resonance (ESR) analysis. The results showed that the peroxidase-like activity of Fe3(PO4)2·8H2O originated from the generation of hydroxyl radical (•OH). In addition, we have established a platform for colorimetic detection H2O2 and glucose. As low as 1.0×10− 3 mmol L− 1 H2O2 and 5.0×10− 3 mmol L− 1 glucose could be dectected via our method. Moreover, this sensitive method was successfully applied for the determination of glucose in human serum.
Unveiling the Role of CD27+ Memory B Cells in Primary Biliary Cholangitis: A Mendelian Randomization Study
Authors: He Zheng-Jie , Zhang Peng-Wei , He Ke , Shi Zhi 
Published: January 2024

Abstract
This study performed a two-sample Mendelian randomization (MR) approach based on genome-wide association study (GWAS) summary statistics to investigate the causal relationship between immune cells and primary biliary cholangitis (PBC). A total of 731 immune cell traits were evaluated for association with PBC to identify diagnostic biomarkers and potential therapeutic targets. GWAS data on immune cell traits and PBC were collected with Single Nucleotide Polymorphisms (SNPs) used as instrumental variables. The IVW model showed a causal relationship between elevated levels of four CD27+ memory B cell types and increased risk of PBC (PFDR < 0.05). Specifically, CD27 expression on CD24+ CD27+ B cells, IgD+ CD24+ B cells, IgD− CD38dim B cells and unswitched memory B cells showed associations with PBC risk. However, no causal relationship between PBC and these immune cell traits was observed in the reverse MR analysis. Differential gene analysis from the Gene Expression Omnibus (GEO) database, Spearman correlation analysis and enrichment analysis further supported the association between CD27+ memory B cells and PBC risk. These findings suggest that CD27 + memory B cells play a role in the pathogenesis of PBC and may serve as important targets for diagnostic and therapy strategies.
Genetic Assessment of Productive and Reproductive Traits in Friesian, Native, and Crossbred Cattle in Egypt
Authors: Ghazy Abdullah Ali , El-Enin Abdelhamid Saeed Abo , Badr Anas Abd El-Salam Abou El-Anine , El-Awady Hassan Ghazy , El-Naser Ibrahim Atta Mohammed Abu 
Published: January 2024

Abstract

A total of 3023, 596, and 1189 lactation records for Friesian, Native, and their Crosses cows from 1994 to 2015 were utilized in this study to estimate and assess genetic and phenotypic parameters and breeding values for 305-day milk yield (305-DMY), lactation period (LP), calving interval (CI), and days open (DO) in these three genotype groups within the Egyptian context. Data were analyzed using the LSMLMW and MTDFREML programs. The analytical model included fixed effects such as season and year of calving, parity, and genotype groups, while random effects included animal and error. Unadjusted means for 305-DMY, LP, CI, and DO were as follows: 3597 kg, 362 days, 524 days, and 198 days for Friesian cows; 1399 kg, 199 days, 499 days, and 169 days for Native cows; and 2671 kg, 395 days, 556 days, and 215 days for Crosses cows. Genotype groups had a highly significant impact on all the studied traits. Heritability estimates were higher in both Crosses (0.32, 0.26, 0.25, 0.23) and Native cows (0.26, 0.28, 0.28) for productive and reproductive traits, respectively, compared to the Friesian cows (0.24, 0.22, 0.16, 0.17) for the same traits. Genetic correlations among productive and reproductive traits ranged from 0.10 to 0.86 for the three genotype groups, while corresponding phenotypic correlations were small to moderate and positive. The accuracy estimated for breeding values indicated that genetic improvement can be achieved through both sires and cows.
Enhancing beef cow grazing productivity during summer: influence of tree density on beef cow and calf performance in natural grasslands
Authors: Llanos Pablo N. , Gonzalez C. M. , Fedrigo Jean K. , Viñoles Carolina 
Published: January 2024

Abstract
To study the impact of tree density on the productivity of beef cows and their calves grazing natural grasslands during the summer, 29 cows (Brangus and their crossbreeds with British breeds) and their calves were randomly assigned to 3 treatments: 1) Full sun (0 trees/ha), 2) Low density (160 trees/ha), 3) High density (240 trees/ha). Grazing intensity was managed through its height at 7-10 cm in all treatments. Traits evaluated included live weight, body condition score, milk production, pregnancy rate and black globe temperature and humidity index (BGTHI), that were analysed using the SAS mixed and genmode procedures, being significant if P<0.05 and tendencies for P values >0.05 and <0.1. Each cm of forage height was equivalent to 168 kg DM/ha. The lowest BGTHI was recorded under the trees in the treatment 160 trees/ha (69.9±1.4), but the value in the alley of treatment 240 trees/ha (72.1±0.7) was similar to the control (72.2±0.7). Cow and calf productivity was similar among treatments (P>0.05). Cow in the high tree density tended to have a greater pregnancy rate (7/11) than cows in the full sun (2/9), with intermediate values for those in the low tree density (4/9; P<0.06). We conclude that in plantations of 160 and 240 trees/ha of 2 years of age, the cow-calf pair has the same productivity as the treeless group, when the natural grassland height is managed at 9±2 cm. However, pregnancy was positively related to tree density, advantage that was not associated to a lower BGTHI.
Single-Molecule Phosphorescence Resonance Energy Transfer for NIR Targeted Cell Imaging
Authors: Liu Yu , Zhou Xiaolu , Bai Xue , Zhang Heng-Yi , Wang Li-Hua 
Published: January 2024

Abstract

A single-molecule phosphorescence resonance energy transfer (PRET) system with a large Stokes shift of 367 nm and near-infrared (NIR) emission is constructed by alkyl-bridged methoxy-tetraphenylethylene-phenylpyridines derivative (TPE-DPY), cucurbit[n]uril (CB[n], n = 7,8), and β-cyclodextrin modified hyaluronic acid (HACD). The experiment results demonstrate that the high binding affinity and various stoichiometric ratios of CB[n] (n = 7, 8) to TPE-DPY not only regulate the topological morphology of supramolecular assembly but also induce different phosphorescence emissions. The assembly of TPE-DPY and CB[7] presents spherical nanoparticles, exhibiting an emerging phosphorescence emission at 525 nm via the macrocyclic confinement effect to phenyl-pyridine units. CB[8] with a larger hydrophobic cavity binds with TPE-DPY to form an n:n pseudorotaxane nanorod, which induces an efficient phosphorescence at 545 nm. Varying from the binary assembly of CB[7] or CB[8], an entirely distinct topological organic three-dimensional nanoplate is obtained by the co-assembly TPE-DPY with CB[7]/CB[8], accompanying enhanced phosphorescence at 540 nm. Uncommonly, the secondary assembly of HACD and TPE-DPY/CB[7]/CB[8] activates a single intramolecular PRET process derived from phenyl pyridines unit to methoxy-tetraphenylethylene function group, enabling an NIR delayed fluorescence at 700 nm excited by 333 nm, which ultimately applied to mitochondrial targeted imaging for cancer cells.
An atypical case of spondylitis due to non-typhoidal Salmonella in adults: A case report and literature review
Authors: Hamada Toru , Furukawa Shinya , Kubota Masashi , Kikuchi Yoshio , Mitsunaga Eiji 
Published: January 2024

Abstract
Background
Non-typhoidal Salmonella commonly induces intestinal infections; however, spondylitis arising from this bacterium is exceedingly rare. A comprehensive review of the clinical attributes of non-typhoidal Salmonella-induced spondylitis in adult populations is lacking.
Case presentation:
Upon admission to our medical institution, an 83-year-old woman presented with a fever lasting 3 days, accompanied by anorexia and pervasive malaise. A month prior, she had been prescribed celecoxib and had received a trigger point injection. The patient was initially diagnosed with acute pyelonephritis and treated with an antimicrobial regimen. However, a week later, although her fever persisted, a complaint of back pain was absent. Discontinuation of celecoxib led to back pain. Subsequent urine and blood cultures, coupled with magnetic resonance imaging findings, confirmed the diagnosis of pyogenic spondylitis attributable to the Salmonella O7 group. With administration of antimicrobial agents, the patient’s fever abated and her back pain subsided. The antimicrobial regimen continued for 12 weeks, with no resurgence of fever or back pain following treatment.
Conclusions
Spondylitis resulting from non-typhoidal Salmonella is rare, with local pain and fever serving as pivotal diagnostic indicators. It is critical to take an accurate history of non-steroidal anti-inflammatory (NSAIDs) use, such as the use of celecoxib, because of the potential of NSAIDs to obscure symptoms. Blood cultures are equally important, given the propensity to yield positive results in these cultures.