bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromatin organization and behavior in HRAS-transformed mouse fibroblasts
Authors: Otsuka Aoi , Minami Katsuhiko , Higashi Koichi , Kawaguchi Akane , Tamura Sachiko , Ide Satoru , Hendzel Michael J. , Kurokawa Ken , Maeshima Kazuhiro 
Published: November 2023

Abstract
In higher eukaryotic cells, a string of nucleosomes, where long genomic DNA is wrapped around core histones, are rather irregularly folded into a number of condensed chromatin domains, which have been revealed by super-resolution imaging and Hi-C technologies. Inside these domains, nucleosomes fluctuate and locally behave like a liquid. The behavior of chromatin may be highly related to DNA transaction activities such as transcription and repair, which are often upregulated in cancer cells. To investigate chromatin behavior in cancer cells and compare those of cancer and non-cancer cells, we focused on oncogenic-HRAS (Gly12Val)-transformed mouse fibroblasts CIRAS-3 cells and their parental 10T1/2 cells. CIRAS-3 cells are tumorigenic and highly metastatic. First, we found that HRAS-induced transformation altered not only chromosome structure, but also nuclear morphology in the cell. Using single-nucleosome imaging/tracking in live cells, we demonstrated that nucleosomes are locally more constrained in CIRAS-3 cells than in 10T1/2 cells. Consistently, heterochromatin marked with H3K9me3 and H3K27me3 was upregulated in CIRAS-3 cells. Finally, Hi-C analysis showed enriched interactions of the B-B compartment in CIRAS-3 cells, which likely represents transcriptionally inactive chromatin. Increased heterochromatin may play an important role in cell migration, as they have been reported to increase during metastasis. Our study also suggests that single-nucleosome imaging provides new insights into how local chromatin is structured in living cells.
Cantharidin and sodium fluoride attenuate the negative inotropic effects of R-PIA in the isolated human atrium
Authors: Schwarz R. , Hofmann B. , Gergs U. , Neumann Joachim 
Published: November 2023

Abstract
Cantharidin and sodium fluoride inhibit the activity of serine/threonine protein phosphatases 1 and 2A (PP1, PP2A) Cantharidin or sodium fluoride increase force of contraction in human atrial preparation. R-Phenylisopropyladenosine (R-PIA) is agonistic at A1-adenosine receptors. R-PIA exert negative inotropic effects in human atrium. We hypothesized that cantharidin and sodium fluoride would attenuate negative inotropic effects of R-PIA. During open heart surgery, trabeculae carneae from human right atrium were obtained, human atrial preparations (HAP). These trabeculae were mounted in organ baths and electrically stimulated (one beat per second). We studied further isolated electrically stimulated left atrial preparations (LA) and isolated spontaneously beating right atrial preparations (RA) from wild type mice. Force of contraction was recorded under isometric conditions. R-PIA cumulatively applied exerted rapid sustained monophasic concentration- and time-dependent negative inotropic effects in LA and HAP. These negative inotropic effects of R-PIA were attenuated pre-incubation for 30 minutes with either 100 µM cantharidin or 3 mM sodium fluoride (NaF) in HAP but not in LA. In contrast, the negative chronotropic effects of R-PIA in RA were not attenuated by pre-incubation for 30 minutes with either 100 µM cantharidin or 3 mM sodium fluoride. The A1-adenosine signals in a species specific way in the mammalian heart. We hypothesize that R-PIA may exert a negative inotropic effect via serine/threonine phosphatases in the human atrium.
The linked lives in Chinese families: The impact of adult offspring’s divorce on parents’ depressive symptoms
Authors: Zhou Yi , Gao Tingming , Zhang Yi 
Published: November 2023

Abstract
Objectives: In context of prolonged life expectancies and rising divorce rates in China, this study explores how offspring’s divorce is associated with parents’ depressive symptoms.
Methods: Using data from the four waves of Chinese Health and Retirement Longitudinal Study (CHARLS) conducted during 2011-2018, we apply a two-way fixed-effects model with 134,230 observations from 50,264 parent-child pairs to examine the association, perform stratified analyses to investigate the heterogeneity, and replace dependent variable with potential mediators to explore mechanisms.
Results: adult offspring’s divorce is associated with increased parent’s depressive symptoms. The association is more salient for sons’ divorces and more salient among fathers. It’s also more salient for healthier parents in rural areas. After divorce, adult children are more likely to live with parents and their parents make more financial transfers to children.
Discussion: In China, offspring’s divorce impairs their parents’ mental health by affecting intergenerational economic allocations and living arrangements.
Clinical Evaluation of Universal Efficacy of Mouthwashes in Alleviating Pain and Xerostomia Among Nasopharyngeal Carcinoma Patients: A Prospective Cohort
Authors: Mageswaran Rhubain , Ang Zen Yang 
Published: November 2023

Abstract
Purpose
This Prospective study was designed to evaluate the overall efficacy of Mouthwashes in Oral mucositis (OM) related pain and xerostomia in nasopharyngeal carcinoma (NPC) patients undergoing concurrent chemoradiotherapy (CCRT) throughout the course of treatment.
Methods
A prospective longitudinal cohort study enrolled 79 NPC patients receiving CCRT. Patients were interviewed prospectively three times over for pain and xerostomia scores based on the various mouthwashes being used. The highest cumulative score difference was utilised to determine mouthwash superiority.
Results
Fifty-nine participants completed three interviews, during which a total of 490 instances of mouthwash use were observed throughout the treatment period. The median total pain score reduction for Lignocaine mouthwash (112 observations) was 2 (IQR, 3), with a cumulative pain score reduction of 206. For Magic Mouthwash (12 observations), the median total pain score reduction was 1.5 (IQR, 1), with a cumulative pain score reduction of 18. The pain score reduction of these 2 mouthwashes was significantly higher than Benzydamine, Aspirin, Sodium bicarbonate (NaHCO3), and Oral7®. There was no significant difference between the studied mouthwashes in their treatment of xerostomia.
Conclusions
The preferred universal choice for managing OM related pain throughout the entire chemoradiotherapy treatment process for NPC is Lignocaine mouthwash. Magic Mouthwash can be considered as a secondary option or as an additional choice to address OM pain. Combinations of mixed medications, including a local anesthetic, anti-inflammatory, or antihistamine, are suggestive of being effective. There is insufficient evidence to determine the preferred mouthwash for treating OM related xerostomia.
Postoperative atrioventricular block in pediatric patients: impact of congenital cardiac malformations and medications
Authors: loomba rohit , Rausa Jacqueline , Villarreal Enrique , Farias Juan S. , Flores Saul 
Published: November 2023

Abstract
Background
Postoperative atrioventricular block may occur after pediatric cardiac surgery. A small proportion of those who develop atrioventricular block will require pacemaker placement. The primary aim of this study was to determine factors associated with postoperative atrioventricular block. Secondary aims included determining factors associated with pacemaker placement in those with atrioventricular block.
Methods
Data from the PHIS data was utilized to identify patients under 18 years of age who underwent cardiac surgery. Those who did and did not develop atrioventricular block. Univariable analyses and regression analyses were conducted to determine factors associated with postoperative atrioventricular block. Similar analyses were conducted to determine factors associated with pacemaker placement in those with atrioventricular block.
Results
A total of 43,716 admissions were identified. Of these, 2,093 (5%) developed atrioventricular block and 480 (1% of total admissions) underwent pacemaker placement. Approximately 70% of those with atrioventricular block received steroids but this was not associated with a decrease in pacemaker placement. Risk factors (congenital malformations of the heart, comorbidities, medications) associated with increased risk of atrioventricular block and pacemaker placement were identified.
Conclusions
Postoperative atrioventricular block occurred in 5% of pediatric admissions for cardiac surgery. Of these admissions with postoperative atrioventricular block, 23% required pacemaker placement. Isoproterenol and steroids were not associated with a reduction in the likelihood of pacemaker placement.
A non-compromising method for optimizing multi-objective problems
Authors: chen yong , xiaoguang guo , Zhang Junhua , Li Yuecheng , Tong Liang , Guo Chendong 
Published: November 2023

Abstract
Multi-objective optimization often entails the concurrent optimization of multiple objectives, which may exhibit conflicts. In many engineering application fields and machine learning algorithms, when determining the final solution, there is a need for trade-offs among different optimization objectives using weight parameters. Here we propose a novel methodology, called Rise-Dimension Screen(RDS), to screen the optimal solution of multi-objective optimization problems from Pareto Front, without the need for compromising between different optimization objectives. We elevate the deterministic numerical values of design variables (0-dimensional space) to a probability density function (1-dimensional space) based on historical data, thereby raising the corresponding constraint conditions to a high-dimensional space. We evaluate their quality by calculating the probability characteristics of different non-inferior solutions satisfying the high-dimensional space constraints, and define the non-inferior solution with the highest probability of meeting the constraint conditions as the final decision solution.
Eucommiae cortex polysaccharides ameliorates learning and memory deficits in Alzheimer's disease mice by modulating gut microbiota and glutamate metabolism
Authors: Zhao Yongkang , Zhao Wenxing , Chai Xuejun , Zhang Lulu , Huang Junlang , Sun Penghao , Guo Xingrui , Yi Chenju , Zhu Xiaoyan , Zhao Shanting 
Published: November 2023

Abstract
Alzheimer's disease (AD) is a chronic neurodegenerative disorder that is distinguished by impairments in learning and memory. The microbiota-gut-brain axis has emerged as a significant factor in the pathogenesis of Alzheimer's disease. Eucommiae cortex polysaccharides (EPs), one of the most abundant substances in the Eucommiae cortex, exhibit potential immunomodulatory and neuroprotective effects. In our study, we discovered that long-term intervention with EPs (0.25%, w/w in food) significantly improved learning and memory in 5×FAD mice, a well recognized mouse model for Alzheimer's disease. Additionally, EPs substantially reduce the generation and deposition of amyloid-beta (Aβ) plaques in AD Mice. EPs reshaped the composition of gut microbiota, increased the abundance of Erysipelotrichaceae, Allobaculum, Bacteroidota, and Muribaculaceae, which are related to gut immunity and short-chain fatty acids(SCFs) production that exert neuroprotective effects through the gut-brain axis. Furthermore, EPs administration was able to correct metabolic disorders in AD mice, particularly glutamate metabolism disturbances. Correlation analysis demonstrates a robust association between the restructuring of gut microbiota and alterations in glutamate metabolism. Importantly, EPs administration significantly inhibited neuroinflammation and restored the oxidative-antioxidative balance in the brains of AD mice, which are potential factors contributing to learning and memory impairments. Interestingly, EPs administration significantly activated autophagy. Our results demonstrate that EPs reshaped gut microbiota, corrected glutamate metabolism disturbances, and significantly alleviated learning and memory impairments in 5xFAD mice through the gut-brain axis. The natural extract EPs holds promising potential for the therapeutic management of neurodegenerative disorders.
Rock roughness quantization algorithm under the condition of rotary excavation
Authors: Renyi Chen , Yonggao Yin , Min He 
Published: November 2023

Abstract
In the modern pile foundation drilling operation, a series of regular joints will be left on the rock surface during the serration rotation of the drill, and the traditional method to determine the roughness of the joint has great limitations. For more accurately quantify the roughness of rock joint under the condition of rotary drilling, the geometry of rock joint surface is obtained by three-dimensional non-contact high-precision laser scanner. Twenty contours of the joint surface were intercepted along the shear direction on the XZ system plan. The mean height h0 and RMS σ of the center line reflecting the undulation characteristics of the joint surface and the parameter θp reflecting the steepness of the joint surface are extracted by using the contour lines. The method of quantizing roughness with L/L0 is extended to three-dimensional space S/S0, the functional relation between JRC and σ and θp is obtained by bivariate fitting, and the goodness of fit reaches 99.46%. The results of two real examples and laboratory direct shear tests show that the proposed method is close to other methods, and the numerical variation trend is more stable than that of the classical method, which verifies the feasibility of the proposed method.
The effectiveness of the use of topical bacterial lysates in frequently ill children with pharyngeal tonsil hypertrophy and/or exacerbation of chronic adenoiditis
Authors: Vladimirova T. A. , Martynova A. S. 
Published: November 2023

Introduction. The issue of diseases of the lymphopharyngeal ring in childhood remains extremely relevant in the outpatient practice of otorhinolaryngologists.Aim. To study the effectiveness of the use of bacterial lysates (IRS-19, Imudon) in frequently ill children with adenoid hypertrophy, exacerbation of chronic adenoiditis at the outpatient stage of treatment.Materials and methods. Examination and treatment of 67 children (35 girls and 32 boys) with hypertrophy of the pharyngeal tonsil and/or exacerbation of chronic adenoiditis at the age of 3 to 14 years (average age was 8.7 ± 6.2 years) was carried out based on two state polyclinics in Samara during the period from May 2023 to August 2023. The patients were divided into 2 groups matched by sex and age: the control group (n = 33) and the main group (n = 34), which additionally used topical bacterial lysate IRS-19 and a mixture of bacterial lysates Imudon. Complaints were assessed, ENT examination, endoscopic endonasal revision of the nasal cavity and nasopharynx, acoustic impedancemetry was performed, study of smears-prints from the structures of the pharyngeal tonsil, a serological blood test (ELISA) with the determination of IgG and IgM for herpes viruses (types I, II, IV, VI), cytomegalovirus and Epstein-Barr virus were conducted on the first day of treatment, on the 10th day of treatment and in dynamics after 3 months.Results. By the 10th day in the main group, nasal congestion decreased in 73% of cases, discharge from the anterior nasal cavity in 78% of cases; the size of adenoid vegetations decreased and reached grade I in 34.4% of cases, grade II adenoids were detected in 49.3% of cases, in the control group the size of adenoids corresponded to grade II (50.5% of cases) and grade III (34.7% of cases). In dynamics, after 3 months of observation, children in the main group, using bacterial lysates (IRS-19, Imudon), showed a significant decrease in episodes of ARVI and adenoiditis, in contrast to the control group, where the incidence was 2.3 times higher.Discussion. A significant decrease in the frequency of complaints from patients in the main group and a more pronounced decrease in the size of the pharyngeal tonsil were revealed.Conclusions. Thus, the results of the study indicate the therapeutic and preventive effectiveness of including bacterial lysates (IRS-19, Imudon) in complex therapy in children with hypertrophy of the pharyngeal tonsil and/or exacerbation of chronic adenoiditis.
Short-term impacts of a large cultural event on the microbial pollution status of a pre-alpine river
Authors: Leopold Melanie , Krlovic Nikola , Schagerl Michael , Schelker Jakob , Kirschner Alexander K. T. 
Published: November 2023

Abstract

Rivers are impacted by microbial faecal pollution from various sources. We report on a short-term faecal pollution event at the pre-alpine Austrian river Traisen caused by the large cultural event FM4 Frequency music festival, with around 200,000 visitors over 4 days. We observed a massive increase of the faecal indicator bacteria (FIB) intestinal enterococci during the event, while Escherichia coli concentrations were only slightly elevated. This increase poses a significant potential health threat to visitors and people recreating downstream of the festival area. A plausible explanation for the uncoupling of the two FIBs may have been a differential persistence caused by a combination of factors including water temperature, solar radiation, and the excessive presence of personal care products (PCPs) in the river water. However, a potential impact of PCPs on FIB assay performance cannot be ruled out. Our observations are relevant for other intensively used bathing sites; detailed investigations on persistence and assay performance of the FIB in response to different ingredients of PCPs are highly recommended. We conclude that for future festivals at this river or other festivals taking place under similar settings, a more effective management is necessary to reduce deterioration in water quality and minimise health risks.
The Impact of Polycystic Ovary Syndrome (PCOS) on the Risk of Developing
Ovarian Cancer and Thyroid Disorders: A Comprehensive Review
Authors: Mukherjee Payal , Sanyal Somali , Chadha Sonia , Mukherjee Sayali 
Published: November 2023

Abstract:
Polycystic ovary syndrome (PCOS) is a common hormonal disorder that affects women. It is characterized by hyperandrogenism, polycystic ovarian morphology, and other related disorders. It is associated with various health conditions, such as infertility and increased risk of heart problems. Ovarian cancer is also a significant concern, as it is the fifth leading cause of death in women. While there is evidence suggesting a potential association between PCOS and ovarian cancer, the exact nature of this relationship remains unclear. Thyroid disorders, particularly hypothyroidism and Hashimoto's thyroiditis, have also been linked to PCOS. The presence of hypothyroidism can contribute to the development of polycystic ovarian morphology, affecting ovulation and hormone balance. Many works have shown a higher ubiquity of autoimmune thyroid disease in PCOS patients, indicating a potential association between the two conditions. The occurrence of PCOS, hirsutism, and acne underscores the frequency of endocrine disorders in women. This review paper examines the present relevant work on the association between PCOS and ovarian cancer as well as PCOS and thyroid disorders. A systematic literature search was conducted on the internet, such as PubMed, Scopus, and Google Scholar database, to identify peer-reviewed publications pertaining to PCOS, ovarian cancer, and thyroid disorders. While some studies have delineated a significant link between PCOS and ovarian cancer or thyroid disorders, others have yielded inconclusive results. Further research is necessary to establish a definitive causal relationship between these conditions. Understanding the relationship between PCOS, ovarian cancer, and thyroid disorders is crucial for early detection, accurate diagnosis, and effective management of these conditions. Identifying potential risk factors and developing appropriate screening strategies can improve women's health outcomes and reduce the burden associated with these disorders.
Association of Child Growth Failure Indicators With Household Sanitation Practices in India (1998-2021): Spatiotemporal Observational Study
Authors: Jain Lovely , Pradhan Sreya , Aggarwal Arun , Padhi Bijaya Kumar , Itumalla Ramaiah , Khatib Mahalaqua Nazli , Gaidhane Shilpa , Zahiruddin Quazi Syed , Santos Celso Augusto Guimarães , AL-Mugheed Khalid , Alrahbeni Tahani , Kukreti Neelima , Satapathy Prakasini , Rustagi Sarvesh , Heidler Petra , Marzo Roy Rillera 
Published: November 2023

Background
Undernutrition among children younger than 5 years is a subtle indicator of a country’s health and economic status. Despite substantial macroeconomic progress in India, undernutrition remains a significant burden with geographical variations, compounded by poor access to water, sanitation, and hygiene services.

Objective
This study aimed to explore the spatial trends of child growth failure (CGF) indicators and their association with household sanitation practices in India.

Methods
We used data from the Indian Demographic and Health Surveys spanning 1998-2021. District-level CGF indicators (stunting, wasting, and underweight) were cross-referenced with sanitation and sociodemographic characteristics. Global Moran I and Local Indicator of Spatial Association were used to detect spatial clustering of the indicators. Spatial regression models were used to evaluate the significant determinants of CGF indicators.

Results
Our study showed a decreasing trend in stunting (44.9%-38.4%) and underweight (46.7%-35.7%) but an increasing prevalence of wasting (15.7%-21.0%) over 15 years. The positive values of Moran I between 1998 and 2021 indicate the presence of spatial autocorrelation. Geographic clustering was consistently observed in the states of Madhya Pradesh, Jharkhand, Odisha, Uttar Pradesh, Chhattisgarh, West Bengal, Rajasthan, Bihar, and Gujarat. Improved sanitation facilities, a higher wealth index, and advanced maternal education status showed a significant association in reducing stunting. Relative risk maps identified hotspots of CGF health outcomes, which could be targeted for future interventions.

Conclusions
Despite numerous policies and programs, malnutrition remains a concern. Its multifaceted causes demand coordinated and sustained interventions that go above and beyond the usual. Identifying hotspot locations will aid in developing control methods for achieving objectives in target areas.