bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological Characterization of Respiratory Pathogens Using the Multiplex PCR FilmArray™ Respiratory Panel
Authors: Hong Young Jun , Jung Bo Kyeung , Kim Jae Kyung 
Published: January 2024

Abstract
Background: Different pathogens can cause upper respiratory tract infections. Since infections caused by different pathogens result in similar symptoms, it is challenging to identify the pathogen based on symptoms alone. Therefore, diagnostic tests for pathogen identification are important for determining the appropriate treatment plan.
Methods and Results: Samples from patients with upper respiratory tract infections collected between January 2018 and December 2022 at the Dankook University Hospital, Cheonan, South Korea, were retrospectively analyzed for pathogens, co-infection, age, and monthly incidence using the FilmArray™ Respiratory Panel (RP) method. A total of 6,367 samples were analyzed. The most common pathogen was adenovirus (18.9%), followed by influenza virus A (16.5%), parainfluenza virus type 3 (12.3%), human rhinovirus/enterovirus (10.4%), and human metapneumovirus (9.7%). These four pathogens accounted for 58.1% of all detected pathogens. Of the 1,744 pathogens, 393 overlapping infectious pathogens were identified, showing a 22.5% co-infection rate. The positivity rate was 72.3% from the age of 0 to 10 years and 44.1% from December to February.
Conclusions: Traditional culture-based methods commonly used for upper respiratory tract infection diagnostics require skilled personnel and have lengthy processing times. By contrast, the FilmArray™ RP method employed in this study enabled the identification of 20 different pathogens within 2 h, including preprocessing, thus preventing treatment delays and unwarranted antibiotic prescription.
Comparative study on cutting performance of CFRP with flat end milling cutter and circular arc milling cutter in inclined angle milling
Authors: Liu Jiaqiang , Chen Tao , xu wenyuan , Wang Guangyue , Zhang Jianyi 
Published: January 2024

Abstract

Carbon fiber reinforced polymer (CFRP) has excellent mechanical properties and has been widely used in aviation, aerospace, and other fields. Due to the anisotropy of CFRP, it is easy to cause cutter wear during the machining process, resulting in machining defects such as burrs, tearing and delamination. In this paper, a comparative experimental study on the inclined angle milling of CFRP with flat end milling cutter and circular arc milling cutter is carried out. The axial force, cutter wear, the quality of hole entrance and exit and the hole wall micro-morphology of the two cutters under the inclined angle milling method were analyzed. The results show that compared with the tip area of flat end milling cutter, the arc transition area of circular arc milling cutter increases the contact area with the workpiece and reduces the milling axial force. The cutter wear of circular arc milling cutter is mainly concentrated in the arc transition area, and the wear gradually diffuses to the nearby cutting edge. However, the wear of flat end mill cutter is mainly concentrated in the cutter tip area. As the number of holes increases, the cutter tip gradually becomes blunt, and the wear area further expands to the area near the cutter tip. With the increase of cutter wear, the entrance defects of the processing hole of the two cutters are less, the exit of the hole of the circular arc milling cutter has defects such as tearing and burrs. In addition, there are defects such as fiber exposed and cavities on the surface of the hole wall. Compared with the circular arc milling cutter, the flat end milling cutter has more defects at the exit of the hole. At the same time, many surface cavities, pits, and fiber pull-out phenomena on the surface of the hole wall.
Short-term and long-term outcomes of Laparoscopic surgery for locally recurrent rectal cancer: A propensity score-matched cohort study
Authors: Zhang Jinzhu , Huang Fei , Niu Ruilong , Mei Shiwen , Quan Jichuan , Hu Gang , Li Bo , Zhuang Meng , Guo Wei , Wang Xishan , Tang Jianqiang 
Published: January 2024

Abstract

Background
Radical surgery remains the primary option for locally recurrent rectal cancer (LRRC) as it has the potential to considerably extend the patient's lifespan. At present, the effectiveness of laparoscopic surgery for LRRC remains unclear.
Methods
The clinical data of LRRC patients who were admitted to the Cancer Hospital of the Chinese Academy of Medical Sciences between 2015 and 2021 were retrospectively analyzed in this study. Patients were categorized into two groups, namely the open group and the laparoscopic group, based on the surgical method used. The short-term outcomes and long-term survival between the two groups were compared.
Results
Curative surgery was performed on 111 patients who were diagnosed with LRRC. After propensity score matching, a total of 84 patients were included and divided into the laparoscopic group (42 patients) and the open group (42 patients). The laparoscopic group had less intraoperative bleeding (100 vs. 300, P = 0.023), a lower postoperative complication rate (19.0% vs. 42.9%, P = 0.018), and a lower incidence of wound infection (0 vs. 14.3%, P = 0.026). Additionally, the laparoscopic group had a higher R0 resection rate than the open group (92.9% vs. 83.3%, P = 0.313), as well as a shorter length of hospital stay (9.5 vs. 11.5 days, P = 0.304), although these differences were not statistically significant. The laparoscopic group had higher 3-year overall survival (86.3% vs. 58.9%, P = 0.022) and 3-year disease-free survival (60.6% vs 32.7%, P = 0.015).
Conclusions
In comparison to open surgery, laparoscopic surgery is linked to less bleeding during the operation, quicker recovery after the surgery, and a lower incidence of infections at the surgical site. Moreover, laparoscopic surgery for LRRC might yield superior long-term survival outcomes.
Clinical Features and Follow-up Outcomes of Individualized Low-dose DOAC Therapy in Chinese Patients Aged 60 Years and Older: A Single-center Study
Authors: Zhang Ruiqi , Du Jiali , Liu Mei-Lin 
Published: January 2024

Abstract
Introduction: It is common to adjust direct oral anticoagulant (DOAC) dosage individually according to the clinical characteristics and coagulation indexes of patients in clinical practice, but its effectiveness and safety are still controversial. The purpose of this study was to analyze the clinical characteristics and follow-up outcomes of elderly patients who adjusted anticoagulant therapy according to coagulation index.
Method: Included were patients who were admitted to the geriatric Department of Peking University First Hospital from January 1, 2016 to December 31, 2021, with indications of anticoagulation therapy, receiving DOAC (Dabigatran, Rivaroxaban) therapy, individualized dose adjustment according to APTT (peak value of 46-60s) or AXA (peak value of 0.5-1.0IU/ml), aged ≥60 years, and complete clinical data. Outpatient or telephone follow-up every three months after discharge until termination or death or end of study (December 31, 2022). The clinical features and follow-up results of Dabigatran 110mg BID group and Dabigatran 110mg QD group, Rivaroxaban 5mg BID group and Rivaroxaban 2.5mg BID group were compared.
Result: A total of 388 patients were enrolled, including 145 (35.1%) in the Dabigatrangroup and 243 (58.8%) in the rivaroxaban group. The Dabigatrangroup was divided into the 110mg BID group (85 cases) and the 110mg QD group (60 cases), and patients in the 110mg QD group were older, lighter, and had lower glomerular filtration rate (eGFR). The Rivaroxaban group was divided into the 5mg BID group (134 cases) and the 2.5mg BID group (109 cases), and patients in the 2.5mg BID group were older, weighed less, had lower activity of daily living (ADL) scores, and had lower eGFR. The mean follow-up time was 49.5±23.4 months in the Dabigatrangroup and 32.1±20.1 months in the Rivaroxaban group. Survival analysis of bleeding events, thrombotic events, and death was not significantly different between Dabigatran 110mgBID and 110mgQD and Rivaroxaban 5mgBID and 2.5mgBID groups.
Conclusion: The clinical characteristics of patients with individualized low-dose DOAC regimen were as follows: age ≥80 years old, weight ≤60kg, ADL score ≤60 points, HAS-BLED score ≥3 points, eGFR < 60ml/min/1.73m², baseline hemoglobin < 120g/L. There was no significant difference in the incidence of bleeding and thrombotic events between individualized low-dose therapy based on patient clotting indicators and standard therapy.
Predictive markers of treatment response to neoadjuvant systemic therapy with dual HER2-blockade
Authors: Bae Soong June , Kim Jee Hung , Lee Min Ji , Baek Seung Ho , Kook Yoonwon , Ahn Sung Gwe , Cha Yoon Jin , Jeong Joon 
Published: January 2024

Abstract
Background
In patients with human epidermal growth factor receptor 2 (HER2)-positive breast cancer, achievement of pathologic complete response (pCR) is a known prognostic indicator after neoadjuvant systemic therapy (NAST). We investigated the clinicopathological factors associated with pCR in patients with HER2-positive breast cancer treated with dual HER2-blockade.
Methods
In this retrospective study, 348 patients with HER2-positive breast cancer were included, who received NAST with docetaxel and carboplatin, combined with trastuzumab and pertuzumab (TCHP). We assessed the effects of HER2 protein expression (3 + vs. 2 + on immunohistochemistry [IHC]) and tumor-infiltrating lymphocyte (TIL) levels (< 30%, low; ≥ 30%, or high) on pCR (ypT0/is and ypN0).
Results
Of the 348 patients with HER2 protein expression data, 278 (79.9%) had HER2 IHC 3+. Among the 305 patients with available TIL data, 121 (39.7%) had high TIL levels. A higher pCR rate was observed in patients with HER2 IHC grade 3+ (71.6% vs. 34.3%, p < 0.001) and high TILs levels (71.9% vs. 57.6%, p = 0.011). After adjusting other clinicopathologic factors, the HER2 IHC 3+ (Odds ratio [OR], 3.66; 95% confidence interval [CI], 1.92–6.96; p < 0.001), high TIL (OR, 1.94; 95% CI, 1.09–3.45; p = 0.024), and hormone-receptor (HR)-negative (OR, 3.33; 95% CI, 1.88–5.91; p < 0.001) were found to be independently associated with pCR. In HR-positive cases, HER2 IHC 3 + and high TILs levels remained independent predictors of pCR but not in HR-negative breast cancer.
Conclusions
Our results suggest that high HER2 protein expression and TIL predict treatment response to neoadjuvant TCHP, especially in HR-positive breast cancer.
The Effect of Cessation of Drinking Water Fluoridation on Dental Restorations and Crowns in Children Aged 3-5 Years in Israel – A Retrospective Study
Authors: Nezihovski Shiran Shemesh , Findler Mordechai , Chackartchi Tali , Mann Jonathan , Haim Doron , Tobias Guy 
Published: January 2024

Abstract

Background: Community water fluoridation (CWF) began in the 1945 as a public health measure to prevent and control caries and was implemented in Israel in 1981. CWF reduced caries significantly, but in 2014, the Ministry of Health decided to stop CWF in Israel.
The aim of our study was to examine the effect of fluoridation cessation on the dental health of children aged 3-5, treated in "Assuta Tel Aviv" dental clinics, under general anesthesia or deep sedation.
Methods: The computerized Maccabi-Dent database provided data for this retrospective study. Records from the years 2014-2019 including treatment codes for procedures relevant to the study, the number of stainless-steel crowns and restorations of all types were examined. Kruskal-Wallis test was performed to compare the results from before and after fluoridation cessation.
Results: A statistically significant increase in the mean number of treatments in the years after fluoridation cessation (P<0.05) was found. There was approximately a two-fold increase in the number of all treatments for all ages.
Conclusion: The results of the study emphasize the advantages of water fluoridation and are further proof of need to restore community water fluoridation in Israel.
Germinal center B-cell subgroups in the tumor microenvironment cannot be overlooked: their involvement in prognosis, immunotherapy response, and treatment resistance in head and neck squamous carcinoma
Authors: Lin Li , Zou Jiani , Pei Shengbin , Huang Wenyi , Zhang Yichi , Zhao Zhijie , Ding Yantao , Xiao Can 
Published: January 2024

Abstract
Background
More than 60% of patients with head and neck squamous carcinoma (HNSCC) are diagnosed at advanced stages and miss radical treatment. This has prompted the need to find new biomarkers to achieve early diagnosis and predict early recurrence and metastasis of tumors.
Methods
Single-cell RNA sequencing (scRNA-seq) data from HNSCC tissues and peripheral blood samples were obtained through the Gene Expression Omnibus (GEO) database (GSE164690) to characterize the B-cell subgroups, differentiation trajectories, and intercellular communication networks in HNSCC and to construct a prognostic model of the associated risks. In addition, this study analyzed the differences in clinical features, immune cell infiltration, functional enrichment, tumor mutational burden (TMB), and drug sensitivity between the high- and low-risk groups.
Results
Using scRNA-seq of HNSCC, we classified B and plasma cells into a total of four subgroups for the first time: naive B cells (NBs), germinal center B cells (GCBs), memory B cells (MBs), and plasma cells (PCs) and analyzed each subgroup separately. Pseudotemporal trajectory analysis revealed that NBs and GCBs were at the early stage of B cell differentiation, while MBs and PCs were at the end stage. Cellular communication revealed that GCBs acted on tumor cells through the CD99 and SEMA4 signaling pathways. By employing univariate Cox regression, Lasso, and multivariate Cox regression analysis, we developed several risk scores based on marker genes of GCBs subgroups, especially the independent prognostic value of MEF2B+ GCB score was validated. The results of immune cell infiltration, TMB, and drug sensitivity assays were significantly different in HNSCC samples from high and low- MEF2B+ GCB score groups.
Conclusions
This study provides ideas to unravel the fundamental biological functions of B cells and their complex mechanistic roles. For the first time, we identified GCBs as B cell-specific prognostic biomarkers. The MEF2B+ GCB score fills the research gap in the genetic prognostic prediction model of HNSCC and is expected to provide a theoretical basis for finding new therapeutic targets for HNSCC.
The unveiling of a dynamic duo; hydrodynamic cavitation and cold plasma for the degradation of furosemide in wastewater
Authors: Verdini Federico , Abramova Anna , Boffa Luisa , Gaudino Emanuela Calcio , Cravotto Giancarlo 
Published: January 2024

Abstract
The degradation in water of furosemide (FUR), a widely used diuretic drug, was herein reported. The method entails an integrated approach based on the hybridisation of hydrodynamic cavitation (HC) with electrical discharge (ED) plasma technology. This dynamic duo could increase the production of oxidising compounds in water, in particular hydroxyl radicals (OH radicals), by triggering the rapid homolytic decomposition of water molecules and avoiding the addition of external oxidants. This study clearly emphasises the effectiveness of an integrated approach to improve the degradation of pollutants in wastewater originating from active pharmaceutical ingredients (APIs). The results of HC/ED-assisted FUR degradation in the presence of radical scavengers highlight the predominant role of the radical oxidation mechanism at the gas-liquid interface of the cavitation bubble during HC/ED treatment. A comparative analysis of the three technologies — HC alone, HC/ED and UV alone — emphasised the promising potential of hybrid HC/ED as a scalable industrial technology. This is demonstrated by the higher degradation rates (100%, 10 min) when treating large volumes (5L) of wastewater contaminated with FUR (50mg/L), even in the presence of other APIs.
Pan‑cancer Analysis of LAP2α as a Potential Prognostic and Immunological Biomarker for Multiple Cancer Types Including Glioma
Authors: Wang Danwen , Yu Donghu , He Yongze , Tang Feng , Pan Zhiyong , Wang Zefen , Ma Chao , Li Zhiqiang 
Published: January 2024

Abstract
LAP2α, a variant of the lamin-associated polypeptide 2 (LAP2) family, is crucial for in the process of nuclear structure organization and maintaining genomic stability. This research was aimed to investigate the involvement of LAP2α in cancer development, especially in glioma. Based on public datasets, we found LAP2α was upregulated in most cancers, and the survival analysis indicated a distinct correlation between elevated LAP2α expression and unfavorable prognosis among glioma patients with elevated levels of macrophage and neutrophil. LAP2α level evidently associated with the immune checkpoint therapy related genes in cancers. Specifically, we made the tissue microarray covered 80 glioma patients with prognostic analysis, and verified that reducing LAP2α hindered the growth and movement capacity of the glioma cells. Our data suggests that LAP2α may be an important tool for indication of immunotherapy and medical prognosis in pan-cancer, and is expected to have a critical role in the oncogenesis of glioma.
ACSS3 promotes the tumorigenesis of non-small cell lung cancer via suppressing p53-mediated ferroptosis
Authors: Zhang Jing , Wang Xiuhong , Wang Jingyi , Wen Xiao , Chen Siyuan , Wang Tao , Wang Bei , Hu Wenquan 
Published: January 2024

Abstract
Purpose
Non-small cell lung cancer (NSCLC) is a subtype of the most frequently diagnosed cancer, causing a considerable number of deaths globally. Mitochondrial dysfunction has been observed to promote malignant progression. However, the underlying mechanism is still unclear. Acyl-CoA synthetase short chain family member 3 (ACSS3) is mainly located in mitochondria, which abnormal regulation is usually accompanied by the occurrence and development of tumors. This study aimed to evaluate the potential role of ACSS3 in the tumorigenesis of NSCLC.
Methods
The impact of ACSS1 and ACSS3 expression levels on the survival rates of NSCLC patients was examined by online database. ACSS3 protein expression in 79 NSCLC samples and 79 adjacent tissues was evaluated by immunohistochemistry (IHC). The effect of ACSS3 on proliferation, migration and invasion of NSCLC and the molecular mechanism were investigated through in vitro experiments and xenograft tumor.
Results
ACSS3 expression level was correlated with the poor prognosis of NSCLC patients. ACSS3 knockdown led to mitochondrial contraction, increased ROS levels, decreased mitochondrial membrane potential, and subsequently inhibited growth of NSCLC cells in vitro and in vivo, while ACSS3 overexpression promoted these processes. Mechanistically, ACSS3 deficiency promoted ferroptosis through transcriptional control of SLC7A11 and GPX4. Further investigations found that loss of ACSS3 inhibited the SLC7A11/GPX4 axis by enhancing the stability of p53.
Conclusion
ACSS3 promotes NSCLC tumorigenesis through inhibiting the p53-mediated ferroptosis. As such, ACSS3 should be considered a new potential therapeutic target for the treatment of NSCLC.
Hematologists’ perspective on Advance Directives, a French national prospective cross-sectional survey – the ADORE-H Study
Authors: Serey Kevin , Cambriel Amélie , Pollina-Bachellerie Adrien , Bay Jacques-Olivier , Bouleuc Carole , Ladrat Laure , Lotz Jean-Pierre , Philippart Francois 
Published: January 2024

Abstract

Background: The onset of hematological malignancy can lead to acute and critical situations. It can also lead to adverse outcome despite the major advancements made in their therapeutic management. In this context, advance care planning and, in particular, advance directives (AD) play an essential role. However, using AD in patients with malignant hemopathies remains very rare.
Methods: The aim was to evaluate the perception of AD by hematologists. We made a French national online survey. All hematologist working in a hospital setting ant treating malignant hemopathies were solicited. The questionnaire covered 5 areas: personal perception of AD; assistance in writing AD; patient information about AD; use of ADs; demographic data.
Results: 318 hematologists (33.7% of the whole population), working in 103 different centers across France participated in the study. 72.6% (n=231) of the respondents considered that AD could be interesting for patient’s care. Only 32.7% talked about AD with their patients on a regular basis. The lack of use was correlated with the fear of creating anxiety for the patient (64.9%; n = 172) or in relatives (30.9%; n = 80), or the fact that AD were deemed to be inappropriate in their patients (57.8%; n = 145). 19.5% (n = 62) of the responding hematologist offer patients their help to write AD. This proportion is higher in physicians who previously work in palliative care unit (35,6 % vs 16,8 %, p = 0,0004).
Interpretation: The majority of the interrogated hematologist have a positive opinion on AD, but only a few of them break the matter to their patients. The fear of patients and relatives’ consequences, especially anxiety, remains the main limit to information about AD.
Prognostic impact of metabolic syndrome in patients with primary endometrial cancer: A retrospective bicentric study
Authors: Shehaj Ina , Krajnak Slavomir , Rad Morva Tahmasbi , Gasimli Bahar , Hasenburg Annette , Karn Thomas , Schmidt Marcus , Müller Volker , Becker Sven , Gasimli Khayal 
Published: January 2024

Abstract
Purpose Endometrial cancer (EC) is the most common gynaecological cancer. Its incidence has been rising over the years with ageing and increased obesity of the high-income countries’ populations. Metabolic syndrome (MetS) has been suggested to be associated with EC. The aim of this study was to assess whether MetS has a significant impact on oncological outcome in patients with EC.
Methods This retrospective study included patients treated for EC between January 2010 and December 2020 in two referral oncological centers. Obesity, arterial hypertension (AH) and diabetes mellitus (DM) were criteria for the definition of MetS. The impact of MetS on progression free survival (PFS) and overall survival (OS) was assessed with log-rank test and Cox regression analyses.
Results Among the 415 patients with a median age of 64, 38 (9.2%) fulfilled the criteria for MetS. The median follow-up time was 43 months.
Patients suffering from MetS did not show any significant differences regarding PFS (36.0 vs. 40.0 months, HR: 1.49, 95% CI 0.79-2.80 P=0.210) and OS (38.0 vs. 43.0 months, HR: 1.66, 95% CI 0.97-2.87, P=0.063) compared to patients without MetS. Patients with obesity alone had a significantly shorter median PFS compared to patients without obesity (34.5 vs. 44.0 months, P=0.029). AH and DM separately had no significant impact on PFS or OS (p>0.05).
Conclusion In our analysis, MetS in patients with EC was not associated with impaired oncological outcome. However, our findings show that obesity itself is an important comorbidity associated with significantly reduced PFS.