bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute ST-segment elevation myocardial infarction with EDTA-dependent pseudothrombocytopenia: a case report and literature review
Authors: Yuan Xiu , Wei Jie , Wang Binyu , Huang Liting , Li Zhengyi 
Published: October 2023

Abstract

Background:Acute ST-segment elevation myocardial infarction (STEMI) has a rapid onset and high mortality which should be diagnosed quickly after admission and given reperfusion treatment as soon as possible. Meanwhile, antiplatelet and anticoagulant therapy play an important role in the treatment of acute myocardial infarction. Pseudothrombocytopenia has been rarely reported. This paper reports a STEMI patient with delayed reperfusion treatment due to EDTA-dependent pseudothrombocytopenia, and provides suggestions on how to identify EDTA-dependent pseudothrombocytopenia, so as to avoid the wrong withdrawal of antiplatelet drugs and the delay of reperfusion treatment in patients with acute myocardial infarction complicated with pseudothrombocytopenia.
Case presentation: An 82-year-old male patient with a medical history of hypertension was admitted to the emergency department (ED) complaining of worsening chest discomfort. ST-segment elevation was noted in the leads V1-V4 and T-wave height tip on the electrocardiogram (ECG) in the ED. The cardiac biomarkers tests at ED revealed that his high-sensitivity troponin level (cTnI) was 0.25ng/L (reference interval:＜0.01ng/mL), and the serum creatine kinase-MB(CK-MB) level was 17.22ng/mL (reference interval:＜5ng/mL), and myoglobin level was 126.02ng/mL (reference interval:＜50ng/mL). Unexpectedly, his initial platelet count with a standard EDTA tube was 18 × 109 /L, and he did not receive reperfusion therapy and any fibrinolytic drug or heparin because of thrombocytopenia. Echocardiogram demonstrated regional hypokinesia of left ventricle. She received bone marrow puncture and platelet antibody examination. Subsequently, platelet clumps were seen in bone marrow blood smears and platelet antibody tests were negative. Blood collection in the heparin tube reveals an accurate platelet count of 166×109/L.However,it was been a week after his admission, he refused the operation and was discharged with the medication after his condition improved.
Conclusions: Thrombocytopenia will bring risks and challenges to the reperfusion treatment of acute ST-segment elevation myocardial infarction. Clinician should be alert to EDTA-dependent pseudothrombocytopenia especially complicated with myocardial infarction. Seeking possible evidence of pseudothrombocytopenia is in favor of early diagnosis which may prevent unnecessary examination as well as over-treatment and especially mismanagement of patients in potentially life-threatening clinical conditions
Composition analysis and toxigenic fungi isolation on the surface of Polygala Radix in China
Authors: Chen Xuyu , Zhao xiangsheng , Chen Haimei , Liu Chang , Yang meihua 
Published: October 2023

Abstract
Purpose
Polygalae Radix (PR) has been widely used in traditional Chinese medicine for decades. Aflatoxins easily contaminate PR during cultivation, harvesting and storage. However, which fungus produces these toxins on the surface PR is unknown. The purpose of the present study was to analyze the composition and toxigenic fungi on the surface of PR to provide a basis for the prevention and control of toxigenic fungi.
Methods and results
Forty-nine exogenous fungi were isolated from the surface of PR. They were identified mainly based on CAM gene/rDNA ITS and phylogenetic analysis. The results showed that all the isolates belonged to four classes: Eurotiomycetes (79.59%), Dothideomycetes (10.20%), Sordariomycetes (6.12%), and Mucoromycetes (4.08%). Forty-nine isolates were identified at the species level, including 24 isolates of Aspergillus spp. (48.98%), 11 isolates of Penicillium spp. (22.46%) and others. Aspergillus spp. were the predominant exogenous fungi on the surface of PR. In addition, three fungi (A. parasiticus GXYZ1-5, A. flavus YZ-2-3 and A. flavus HBYZ1-4) produced aflatoxins, accounting for 8.57% of Aspergillus spp abundance. The concentrations of aflatoxins B1, B2, G1, and G2 produced by the A. parasiticus GXYZ1-5 isolate were 6.83, 1.82, 25.34, and 1.8 μg/mL, respectively. The concentrations of aflatoxins B1, B2, G1, and G2 produced by the A. flavus YZ-2-3 isolate were 493.37, 43.37, 1229.35, and 40.72 μg/mL, respectively. The concentration of aflatoxins B1 produced by the A. flavus HBYZ1-4 isolate was 7.69 μg/mL.
Conclusions
The results of this study provide a better understanding of exogenous fungi on the surface of PR and provide valuable insights for the prevention and control of toxigenic fungi.
Arabidopsis AN3 and OLIGOCELLULA genes link telomere maintenance mechanisms with cell division and expansion control
Authors: Agabekian Inna A. , Abdulkina Liliia R. , Lushnenko Alina Y. , Young Pierce G. , Valeeva Lia R. , Boskovic Olivia , Lilly Ethan G. , Sharipova Margarita R. , Shippen Dorothy E. , Juenger Thomas E. , Shakirov Eugene V 
Published: October 2023

Abstract

Telomeres are conserved chromosomal structures necessary for continued cell division and proliferation. In addition to the classical telomerase pathway, multiple other genes including those involved in ribosome metabolism and chromatin modification contribute to telomere length maintenance. We previously reported that Arabidopsis thaliana ribosome biogenesis genes OLI2/NOP2A, OLI5/RPL5A and OLI7/RPL5B have critical roles in telomere length regulation. These three OLIGOCELLULA genes were also shown to function in cell proliferation and expansion control and to genetically interact with the transcriptional co-activator ANGUSTIFOLIA3 (AN3). Here we show that AN3-deficient plants progressively lose telomeric DNA in early homozygous mutant generations, but ultimately establish a new shorter telomere length setpoint by the fifth mutant generation with a telomere length similar to oli2/nop2a -deficient plants. Analysis of double an3 oli2 mutants indicates that the two genes are epistatic for telomere length control. Telomere shortening in an3 and oli mutants is not caused by telomerase inhibition; wild type levels of telomerase activity are detected in all analyzed mutants in vitro. Late generations of an3 and oli mutants are prone to stem cell damage in the root apical meristem, implying that genes regulating telomere length may have conserved functional roles in stem cell maintenance mechanisms. Multiple instances of anaphase fusions in late generations of oli5 and oli7 mutants were observed, highlighting an unexpected effect of ribosome biogenesis factors on chromosome integrity. Overall, our data implicate AN3 transcription coactivator and OLIGOCELLULA proteins in the establishment of telomere length set point in plants and further suggest that multiple regulators with pleiotropic functions can connect telomere biology with cell proliferation and cell expansion pathways.
Disseminated Talaromyces Marneffei Infection associated with Hemophagocytic Syndrome in a HIV-Negative Patient in Northern China: a case report
Authors: Yang Hui , Liu Ming , Xu Nannan , Yang Lulu , Wen Sai , Wang Shanshan , Qu Chunmei , Xu Ke , Sun Enhua , Cui Wen , Liu Hui , Wang Gang 
Published: October 2023

Abstract
Background: Talaromyces marneffei is endemic in eastern India, southeast Asia, and Guangdong and Guangxi province in China. It’s more common in immunocompromised individuals, especially in HIV patients.
Case presentation: A 66-year-old male who had a history of hypertension resided in Shandong Province (Northern China) was admitted for recurrent fever for one month. The patient suffered recurrent fever, multiple lymphadenopathy, hepatosplenomegaly, back rash, progressive decrease in white blood cells and platelets. Talaromyces marneffei were cultured in peripheral blood and bone marrow after admission, and a suspected fungal structure was found in lymph node pathology. The patient's infection secondary to hemophagocytic syndrome continued to worsen despite antifungal, anti-inflammatory, and symptomatic treatment, leading to death due to multiple organ failure.
Conclusion:Although rare, infections due to Talaromyces marneffei in HIV-negative patients are increasing in recent years and we should be vigilant against "new" infections in nonendemic areas.
Plasmalogens and Octanoylcarnitine Serve as Early Warnings for Central Retinal Artery Occlusion
Authors: Wang Chuansen , Li Yi , Feng Jiaqing , Liu Hang , Wang Yuedan , Wan Yuwei , Zheng Mengxue , Li Xuejie , Chen Ting , Xiao Xuan 
Published: October 2023

Abstract
Central retinal artery occlusion (CRAO) is a kind of ophthalmic emergency which may cause loss of functional visual acuity. However, the limited treatment options emphasize the significance of early disease prevention. Metabolomics has the potential to be a powerful tool for early identification of individuals at risk of CRAO. In our study, we compared the levels of metabolites in arterial and venous samples collected from patients with acute CRAO and control patients. Our aim was to identify potential biomarkers for CRAO by comparing venous difference between CRAO patients and controls and arteriovenous difference between CRAO patients. The comprehensive analysis of metabolites showed that PC(P-18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PC(P-18:0/20:4(5Z,8Z,11Z,14Z)) and octanoylcarnitine were strongly correlated with CRAO. We compared the predictive performance of the clinical parameter model with combined model. The prediction efficiency of the mixed model was significantly better with area under the receiver operating characteristic curve (AUROC) of 0.815. Other evaluations of combined models have also shown their superiority. These results underscored the potency of these three substances as robust predictors of CRAO occurrence. Trial registration number: WDRY2022-K278, date of registration: November 30, 2022.
Atmospheric Concentrations, Seasonal Variations and Health Risk Assessment of PM 2.5 , PM 10 , and SO 2 in Tehran Metropolis, Iran
Authors: Ahmadian Fahimeh , Rajabi Saeed , Azhdarpoor Abooalfazl 
Published: October 2023

Abstract

This study assessed seasonal and annual variations as well as the health risks associated with exposure to PM2.5, PM10, and SO2 in the ambient air of Tehran from 2019 to 2021. The findings revealed that the average annual concentration of PM2.5, PM10, and SO2 varied from 28.24 to 32.34 µg/m3, 69.57 to 82.22 µg/m3, and 14.94 to 17.98 µg/m3, respectively. The amounts of PM2.5 and SO2 were the greatest in the west and southwest, while PM10 was the most abundant in the east and northeast which were above WHO guidelines. In exposure duration scenarios of 8 and 12 hours, the mean hazard quotient (HQ) for PM2.5 and PM10 was >1, suggesting an unacceptable risk to human health. There was no risk to human health according to the mean HQ for SO2 at all exposure periods of 3, 8, and 12 hours. Further evidence that exposure time plays a significant part in health hazards was provided by the fact that the mean HQ values of exposure to PM2.5 and PM10 in exposure times of 3 hours were both <1. The Sobol sensitivity analysis revealed that the PM2.5 concentration in HQ was the most sensitive indicator of the populace.
Reduced belowground allocation of freshly assimilated C contributes to negative plant-soil feedback in successive winter wheat rotations
Authors: Kaloterakis Nikolaos , Kummer Sirgit , Gall Samuel Le , Rothfuss Youri , Reichel Rüdiger , Brüggemann Nicolas 
Published: October 2023

Abstract

Aims
Successively grown winter wheat (WW) is associated with yield reduction, often attributed to the unfavorable soil microbes that persist in the soil through plant residues. How rotational positions of WW affect the allocation of freshly assimilated carbon (C) above and belowground remains largely unknown.
Methods
A 13CO2 pulse labeling rhizotron experiment was conducted in the greenhouse. WW was grown in soil after oilseed rape (W1), after one season of WW (W2), and after three successive seasons of WW (W4). We used an automatic manifold system to measure the δ13C of soil CO2 at six depths and five different dates. δ13C was measured in the dissolved organic C (DOC), microbial and plant biomass pools.
Results
Rotational position strongly influenced the root-derived C. Higher δ13C was found in the soil CO2 of W1 compared to W4, especially in the topsoil during the late growth stage. Higher DOC and microbial δ13C was traced in W1 and W4 compared to W2. The WW biomass was more enriched in 13C in W1 compared to W2 and W4.
Conclusions
Our study demonstrates a potential mechanism through which the rotational position of WW can affect the allocation of freshly fixed C above and belowground.
Feasibility and safety of EGFR-TKI neoadjuvant therapy for EGFR-mutated NSCLC: a meta-analysis
Authors: Yu Zhuchen , Xu Fei , Zou Juntao 
Published: October 2023

Abstract
Background
The role of neoadjuvant epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) targeted therapy for EGFR-mutated non-small cell lung cancer (NSCLC) is unclear. Previous studies have shown that EGFR-TKIs have excellent anti-tumor activity. However, almost all studies on neoadjuvant EGFR-TKI treatment for EGFR-mutated NSCLC have been non-randomized controlled trials with small sample sizes and different methods of statistical analysis, which may lead to a lack of valid metrics to assess the feasibility and safety of neoadjuvant EGFR-TKI treatment. This meta-analysis aimed to assess the efficacy and safety of neoadjuvant EGFR-TKI treatment for NSCLC patients with EGFR mutations.
Methods
Relevant studies were systematically searched in PubMed, Embase, and Web of Science databases. Results including objective response rate (ORR), complete resection rate (R0), downstaging rate, pathological complete response (PCR), major pathological response (MPR), progression-free survival (PFS), overall survival (OS), and adverse events (AEs) were used for further analysis.
Results
This meta-analysis ultimately included 11 studies involving 344 patients with EGFR-positive mutations in NSCLC. In terms of tumor response, the pooled ORR was 57% (95% CI: 42–73%), and in the Osimertinib subgroup, the pooled ORR was 80% (95% CI: 63–98%). Analysis of studies that reported a downstaging rate showed the pooled downstaging rate of 41% (95% CI: 9–74%) and the pooled downstaging rate of 74% (95% CI: 22–100%) in the Osimertinib subgroup. In terms of surgical outcomes, the pooled pCR rate was 3% (95% CI: 0–7%), the pooled MPR rate was 11% (95% CI: 6–17%), and the pooled R0 resection rate was 91% (95% CI: 85–95%). The most common adverse events associated with neoadjuvant therapy were rash and diarrhea. The pooled incidence of any grade of rash was 47.1% (95% CI: 25.4–69.3%), and the pooled incidence of grade ≥ 3 rash was 0.6% (95% CI: 0.0–2.5%). The pooled incidence of diarrhea of any grade was 28.8% (95% CI: 14.4–45.4%), with the pooled incidence of grade ≥ 3 diarrhea of 0.2% (95% CI: 0.0–1.6%). The pooled incidence of ≥ grade 3 adverse events was significantly lower.
Conclusions
Our meta-analysis confirmed the efficacy and safety of neoadjuvant EGFR-TKIs for the treatment of NSCLC patients with EGFR-positive mutations and that third-generation EGFR-TKIs were superior to first- and second-generation EGFR-TKIs in terms of shrinking tumor volume and lowering tumor stage; however, future large-scale and multicenter randomized controlled trials are needed to confirm this conclusion.
Systematic Review Registration:
PROSPERO CRD42023466731.
Wearable MEMS Pressure Sensor with Temperature compensation for Diagnosing Neurological Disorders By Analyzing Spine configuration
Authors: Biswas Sonali , Biswas Moushumi , Guha Koushik 
Published: October 2023

Abstract
MEMS Pressure sensors find wide applications in varied areas and it is a boon especially in Biomedical application. We know that there may be various changes in spine shape of an individual which depends upon the age group, the sleepware, the physical activity, acute injury, spine degeneration and postures in his day to day life. Any deviation in the spine shape from its natural erect position causes tension in the lower back muscles which leads to muscle fatigue and strain in a very short time. This abnormal spinal configuration may give rise to several neurological disorders. Diagnosis of the disorder is a must to arrest the problem from further degradation and proper medical intervention at the earliest. This paper proposes a High performance wearable piezoresistive pressure sensor which can measure the slightest deviation of the spine by measuring the strain developed in the lower back muscle. As piezoresistors are highly sensitive to temperature fluctuations a scheme is proposed for temperature compensation of this sensor. Any pressure above 20mm Hg may be responsible for several neurodegenerative diseases hence requires early diagnosis which can both alert and prevent one from several neurological disorders. The pressure sensing for minimum misalignment should be as low as 15mm of Hg or 1.59kPa to 30mm Hg or 10.6kPa which can cause ischemia or ulcer. The proposed sensor has range from 1kPa to 100kPa for early diagnosis of neurological disorders caused by spinal misalignment. The sensitivity is around 3.5mV/V/kPa.
Identification and screening apple endophytic bacteria applied at different times for biological control of Alternaria rot on apple in Turkey
Authors: Bektas Idris , Yazdıc Ferit Can , Kusek Mustafa 
Published: October 2023

Abstract
In this study, an isolate of Alternaria alternata, the causal agent of apple fruit rot, was isolated from rotten cv Amasya Apple and identified based on ITS gene analysis and morphological analyses. Out of 58 different endophytic bacteria isolated from internal tissues of apples, twenty-six isolates caused the radial growth inhibition of A. alternata in dual cultures. Based on 16S rRNA sequencing analysis, these endophytic antagonistic bacteria were identified as Bacillus ssp. Bacillus methylotrophicus(ib17) exhibited the highest significant inhibitory effect on the mycelial growth of A. alternata at a rate of 79.45% under in vitro conditions. The in vivo analysis revealed that the biocontrol efficiency of the Bacillus licheniformis(ib20) against A. alternata reached 100% with a treatment duration of 24 h before the introduction of A. alternata. When the bacteria and A. alternata were introduced simultaneously, Bacillus amyloliquefaciens(ib1), Bacillus licheniformis(ib21), and Endophytic bacterium (ib16) showed the highest biocontrol effect against A. alternata at 81.8%. Moreover, when the bacteria introduced 24 h after A. alternata, Bacillus licheniformis(ib21) showed the highest biocontrol efficacy at 83.3% against the fungus compared to the controls. The endophytic bacteria could produce fungus cell wall hydrolyzing enzymes such as chitinase, cellulase, amylase, and protease. According to the results of this study, apple endophytic bacteria are a potential candidate to control apple rot caused by A. alternata due to cell wall hydrolyzing enzymes, as well as to reduce disease severity. However, further research needs to be carried out on the biochemical basis of their activity against A. alternata.
Social isolation consequences: lessons from COVID-19 pandemic in a context of dynamic lock-down in Chile
Authors: Patrono Alessandra , Renzetti Stefano , Guerini Cristian , Macgowan Mark , Moncada Stefanny M , Placidi Donatella , Memo Maurizio , Lucchini Roberto G. 
Published: October 2023

Abstract

Background: Chile did not adopt general and unified lockdowns for the whole nation but organized itself with dynamic and sometimes irregular lockdowns. These dynamics and consequences of social isolation could be generalized to other contexts of isolation such as those affecting minorities such as immigrants, prisoners, refugees.
Methods: In this study, we investigated the physical and mental health symptoms associated with lifestyle changes due to lockdown among university students in Chile. We examined psychopathological variations in relation to mental health problems in a healthy young population. Our goal was to develop interventions to address these new psychosocial problems in potentially comparable post-pandemic contexts. From May 10th 2021 to June 2th 2021, 420 University students took part in an anonymous survey asking for information on habits and symptoms that emerged during the lockdown in response to the COVID-19 pandemic. Three health outcomes were assessed: digestive disorders; headache; fear of COVID-19. Covariates including conditions and lifestyle during the pandemic, SARS-CoV-2 infections in the family, financial situation and productivity were considered in the analysis.
Results: Participants experienced headache and fear of COVID-19 quite frequently during the lockdown period. More than half of the sample also experienced social isolation. Female gender, sleep quality, memory difficulties, and a change in eating habits resulted associated with an increased risk of health outcomes such as headaches and digestive disorders.
Conclusions: The results of this study fit within an original pandemic context: The results of this study can help identify needs and promote solutions applicable to different contexts. Future interventions should focus on the promotion and implementation of healthy habits focused on sleep hygiene, psychoeducation on the use of mobile devices and gender medicine with the support of healthcare organizations and University.
Navigating the Crossroads: Sexual Attitudes, HIV Awareness, and the Need for Targeted Education Among College Students in Southeast Coast China.
Authors: Liu Yupeng , Zhu Xuan , Ye Zhenmiao , Ni Xiaoyan , Chen Yuxin , Yu Xinru , Li Dapeng , Wang Shuran 
Published: October 2023

Abstract

Background: College students, as a sexually active group, are particularly vulnerable to the risks of the human immunodeficiency virus (HIV)/ acquired immunodeficiency syndrome (AIDS) acquisition and transmission. Findings and limitations of previous studies on factors affecting HIV/AIDS-related awareness and sexual attitudes support conducting further research in this study. This study aims to describe and evaluate the extent to which college students with different sexual attitudes understand HIV/AIDS in Wenzhou, Zhejiang Province, China, and related factors for HIV/AIDS prevention.
Methods: From November to December 2020, an anonymous questionnaire was conducted among 6,000 college students in Wenzhou, China to assess their sexual attitudes and knowledge towards HIV/AIDS. Descriptive statistics, Chi-square test, and logistic regression were used to analyze the factors affecting HIV/AIDS-related knowledge and sexual attitudes. We also performed a subgroup analysis on sexual attitudes.
Results: A total of 5,627 valid questionnaires were included in our analysis, with an effective rate of 93.8%. The participants’ rate of HIV/AIDS-related awareness was 80.2%. Regarding sexual attitudes, 4,098 (72.8%) students held conservative views, while the rest (27.2%) held more open views. Among conservative students, seniors (AOR=1.12, 95%CI 1.01-1.23) and those with better health education (AOR=1.50, 95%CI 1.28-1.76) had higher HIV/AIDS knowledge. Conversely, rural students had lower HIV/AIDS knowledge (AOR=0.79, 95%CI 0.67-0.94). Additionally, in the open groups, students with better health education (AOR=1.21, 95%CI 1.11, 1.31) demonstrated higher levels of HIV/AIDS-related knowledge, whereas rural students (AOR=0.67, 95%CI 0.51, 0.89) showed lower HIV/AIDS knowledge.
Conclusion: A significant portion of college students still possessed a permissive and hazardous sexual mindset and demonstrated a lack of HIV/AIDS-related knowledge. It’s crucial to hunt for ways to level students’ awareness of HIV/AIDS and provide them with more comprehensive sex education.