bluuebunny/crossref_metadata_2025_split huggingface dataset:
CLEC12A sensitizes differentially responsive breast cancer cells to the anti-cancer effects of artemisinin by repressing autophagy and inflammation
Authors: Chatterjee Ranodeep , Shukla Aditya , Chakrabarti Kausiki , Chatterji Urmi 
Published: December 2023

BackgroundEnhanced inflammatory responses promote tumor progression by activating toll-like receptors (TLRs), which in turn are inhibited by C-type lectin like receptors (CTLRs), like CLEC12A. Although the presence of CLEC12A in acute myeloid leukemia is well established, its role in non-hematopoietic tumors is still obscure. In hematopoietic tumors, CLEC12A mostly inhibits TLRs and modulates inflammatory responses via NF-κB signaling. In this study, the fate of tumor progression was determined by modulating CLEC12A using artemisinin (ART), a FDA-approved anti-malarial drug, known for its anti-cancer and immunomodulatory properties with minimal adverse effects on normal cells.MethodEffects of ART were primarily determined on hematological factors and primary metastatic organs, such as lungs, kidney and liver in normal and tumor-bearing BALB/c mice. Tumor-bearing mice were treated with different concentrations of ART and expressions of CLEC12A and associated downstream components were determined. CLEC12A was overexpressed in MDA-MB-231 and 4T1 cells, and the effects of ART were analyzed in the overexpressed cells. Silencing TLR4 using vivo morpholino was performed to elucidate its role in tumor progression in response to ART. Finally, CLEC12A modulation by ART was evaluated in the resident cancer stem cell (CSC) population.ResultsART did not alter physiology of normal mice, in contrast to tumor-bearing mice, where ART led to tumor regression. In addition, ART reduced expression of CLEC12A. Expectedly, TLR4 expression increased, but surprisingly, that of NF-κB (RelA) and JNK/pJNK decreased, along with reduced inflammation, reduced autophagy and increased apoptosis. All the above observations reverted on overexpression of CLEC12A in MDA-MB-231 and 4T1 cells. Inhibition of TLR4, however, indicated no change in the expressions of CLEC12A, NF-κB, or apoptotic markers. The effect of ART showed a similar trend in the CSC population as in cancer cells.ConclusionThis study, for the first time, confirmed a differential role of CLEC12A in non-hematopoietic tumor and cancer stem cells in response to ART. Subsequent interaction and modulation of CLEC12A with ART induced tumor cell death and abrogation of CSCs, confirming a more comprehensive tumor therapy with reduced risk of recurrence. Therefore, ART may be repurposed as an effective drug for cancer treatment in future.
A real-world disproportionality analysis of mepolizumab based on the FDA adverse event reporting system
Authors: Li Huqun , Wang Chongshu , Deng Aiping , Guo Cuilian 
Published: December 2023

Background: Mepolizumab has been approved by the FDA for add-on maintenance treatment of severe asthma with an eosinophilic phenotype. Real-world studies on mepolizumab-associated adverse events are limited. The present study aimed to explore mepolizumab-related adverse events based on the US Food and Drug Administration Adverse Event Reporting System (FAERS) database.Methods: A disproportionality analysis was performed to assess the safety profile of mepolizumab based on the reports from the FAERS database between October 2015 and December 2022. Demographic information, the time to onset, the safety of long-term mepolizumab exposure as well as safety in pediatric patients were also investigated.Results: A total of 736 significant preferred terms (PTs) were identified among the 13,497 mepolizumab-associated adverse events (AEs) reports collected from the FAERS database. The frequently reported AEs including dyspnea, fatigue, and headache were in line with drug instruction and previous studies. Unexpected significant AEs such as cough, malaise, and chest discomfort were also identified. Most AEs occurred within the first month after mepolizumab initiation. Pneumonia and wheezing were frequently reported in patients with long-term mepolizumab exposure as well as in the pediatric population.Conclusion: Our results were consistent with the observations in previous clinical and real-world studies. New and unexpected AE signals of mepolizumab were also identified. Close attention should be paid to the long-term safety of mepolizumab as well as safety in the pediatric population. Prospective studies are required for optimal use of mepolizumab.
Berberine-microbiota interplay: orchestrating gut health through modulation of the gut microbiota and metabolic transformation into bioactive metabolites
Authors: Dehau Tessa , Cherlet Marc , Croubels Siska , Van De Vliet Michiel , Goossens Evy , Van Immerseel Filip 
Published: December 2023

Berberine is an isoquinoline alkaloid found in plants. It presents a wide range of pharmacological activities, including anti-inflammatory and antioxidant properties, despite a low oral bioavailability. Growing evidence suggests that the gut microbiota is the target of berberine, and that the microbiota metabolizes berberine to active metabolites, although little evidence exists in the specific species involved in its therapeutic effects. This study was performed to detail the bidirectional interactions of berberine with the broiler chicken gut microbiota, including the regulation of gut microbiota composition and metabolism by berberine and metabolization of berberine by the gut microbiota, and how they contribute to berberine-mediated effects on gut health. As previous evidence showed that high concentrations of berberine may induce dysbiosis, low (0.1 g/kg feed), middle (0.5 g/kg feed) and high (1 g/kg feed) doses were here investigated. Low and middle doses of in-feed berberine stimulated potent beneficial bacteria from the Lachnospiraceae family in the large intestine of chickens, while middle and high doses tended to increase villus length in the small intestine. Plasma levels of the berberine-derived metabolites berberrubine, thalifendine and demethyleneberberine were positively correlated with the villus length of chickens. Berberrubine and thalifendine were the main metabolites of berberine in the caecum, and they were produced in vitro by the caecal microbiota, confirming their microbial origin. We show that members of the genus Blautia could demethylate berberine into mainly thalifendine, and that this reaction may stimulate the production of short-chain fatty acids (SCFAs) acetate and butyrate, via acetogenesis and cross-feeding respectively. We hypothesize that acetogens such as Blautia spp. are key bacteria in the metabolization of berberine, and that berberrubine, thalifendine and SCFAs play a significant role in the biological effect of berberine.
In search of biomarkers for low back pain: can traction therapy effectiveness be prognosed by surface electromyography or blood parameters?
Authors: Ratajczak Marzena , Waszak Małgorzata , Śliwicka Ewa , Wendt Michał , Skrypnik Damian , Zieliński Jacek , Krutki Piotr 
Published: December 2023

Background: Lumbar traction therapy is a common method to reduce low back pain (LBP) but is not always effective. The search for biomarkers that would prognose the effectiveness of LBP management is one priority for improving patients’ quality of life.Objectives: 1) To determine the phenotype of patients benefiting most from lumbar traction therapy. 2) To correlate systemic and electromyographic biomarkers with pain and pain-related disability.Methods: Data on muscle bioelectrical activity (surface electromyography [SEMG]) in the flexion-extension task, the concentrations of twelve systemic biochemical factors, LBP intensity (Visual Analog Scale), the Oswestry Disability Index, and the Roland–Morris Disability Questionnaire (RMDQ) were collected before and 72 h after 20 sessions of lumbar traction therapy. Patients were divided into responders and nonresponders based on the criterion of a 50% reduction in maximal pain.Results: The responders had lower maximal muscle bioactivity in the extension phase on the left side (p &lt; 0.01) and higher flexion-extension ratios on both sides of the body in the SEMG (left: p &lt; 0.05; right: p &lt; 0.01), and higher adipsin, interleukin-2, interleukin-4, and interleukin-10 concentrations (p &lt; 0.05) than nonresponders. Patients with higher interleukin-4 concentrations before therapy achieved greater reductions in maximal pain in the sitting position, bioelectrical muscle activity in flexion, and flexion-relaxation ratio on the left side of the body. Changes in adipsin and interleukin-4 concentrations correlated with changes in LBP intensity (r = 0.68; r = −0.77). Changes in stem cell growth factor and interleukin-17A correlated with changes in RMDQ (R = 0.53) and bioelectrical muscle activity in extension (left: R = −0.67; right: R = −0.76), respectively.Conclusion: Responders to traction therapy had SEMG indices of less favorable muscle activity in the flexion-extension task and elevated indices of inflammation before the study. For the first time, interleukin-4 was indicated as a potential biomarker for prognosing post-therapy changes in pain intensity and muscle activity.
Prevalence of sexual dysfunction and its association with psychological symptoms in drug-naive major depressive disorder patients in West China
Authors: Jiang Fugui , Liu Zenghui , Wu Xianglong , Tan Arui , Qin Xiaohong , Su Rong Cheng , Li Hui , Wang Huan , Xiao Jun , Zhou Bo 
Published: December 2023

BackgroundSexual dysfunction is commonly observed in individuals with Major Depressive Disorder (MDD), along with various psychological symptoms such as anxiety, somatic complaints, interpersonal sensitivity, and obsessive-compulsive tendencies. However, there is a research gap in understanding the impact of these psychological symptoms on sexual functioning in MDD. Furthermore, there is limited data on the incidence of sexual dysfunction among drug-naive MDD patients in West China. This study aims to determine the prevalence of sexual dysfunction in this patient population and explore its association with other psychological indicators.MethodsWe conducted a retrospective analysis of patient data from October 2020 to September 2022 using propensity score matching. A focused group of 165 males and 490 females was selected from a total of 1941 MDD patients. This allowed for a comparative analysis of demographic data, as well as scores from the Self-Rating Depression Scale (SDS), Self-Rating Anxiety Scale (SAS), and Symptom Checklist-90 (SCL-90), the Arizona Sexual Experience Scale (ASEX).ResultsOur findings reveal that 46.2% of drug-naive MDD patients experienced sexual dysfunction. Notably, there was a higher prevalence of sexual dysfunction among female patients (50.3%) compared to males (37.5%). MDD patients without sexual dysfunction consistently exhibited higher SDS scores than those with sexual dysfunction (p &lt; 0.01), There were no statistically significant differences between male and female MDD patients with or without concomitant sexual dysfunction in terms of Somatic complaints, Obsessive-compulsive, Interpersonal sensitivity, Anxiety, Phobic anxiety, Paranoid ideation, Psychoticism and Diet/sleep difficulties (p &gt; 0.05). In addition, male MDD patients with sexual dysfunction showed a emerging trend towards elevated Hostility scores on the SCL-90 (p = 0.058), male MDD patients with sexual dysfunction showed an increasing trend in hostility scores on the SCL-90, whereas female MDD patients with sexual dysfunction did not show such a trend.ConclusionThe study highlights a significant gender difference in the prevalence of sexual dysfunction among MDD patients, with females being more susceptible than males. There is a positive correlation between the severity of depression and sexual dysfunction in both genders. Interestingly, male MDD patients demonstrated a potential protective effect of hostility against sexual dysfunction, which was not observed in female patients.
Relationship between sleep quality and subjective well-being: resilience as a mediator and belief in a just world as a moderator
Authors: Su Peng , He Mu 
Published: December 2023

Background/PurposeSleep quality significantly impacts subjective well-being, yet its underlying mechanisms remain largely unknown from a scholarly perspective. Existing research has inadequately addressed the relationship between sleep quality and the subjective well-being of College students. This study primarily investigates the influence of sleep quality on the subjective well-being of College students and explores the mediating role of resilience and the moderating role of belief in a just world.MethodsThe study sample comprises 3349 enrolled College students. Measures include the Pittsburgh Sleep Quality Index, the Subjective Well-being Scale, resilience scale, and belief in a just world scale. A moderated mediation model is employed to verify the mediating role of resilience and the moderating role of belief in a just world.Results(1) Sleep quality among College students is significantly positively correlated with resilience, belief in a just world, and subjective well-being. (2) Sleep quality positively predicts subjective well-being among College students. Resilience among College students serves as a mediator between sleep quality and subjective well-being, while belief in a just world moderates the influence of resilience on subjective well-being.ConclusionThe results suggest that sleep quality can directly enhance the subjective well-being of College students and can also indirectly affect it through resilience. Additionally, belief in a just world can enhance the promoting effect of resilience on the subjective well-being of College students. These findings may contribute to understanding the impact of sleep quality on the subjective well-being of College students and its pathways. These research findings can serve as a reference for improving the subjective well-being of College students.
The Local Heroes Project: a youth-led pan-India hyperlocal crisis relief model during the COVID-19 pandemic
Authors: Patil Dhrumil , Shah Priyansh , Rao Shirish , Arora Anoushka , Shah Devarsh , Sarangi Pratik , Gogoi Krittika , Dutta Manaswi , Thakore Yashsri , Pawar Srushti , Zhang Zhenyu , Swaroop Mamta 
Published: December 2023

IntroductionThere was shortage of essential diagnostic and therapeutic supplies in public hospitals during the second wave of COVID-19 in India.Materials and methodsThe Local Heroes Project, a hyperlocal project initiated by the World Youth Heart Federation (WYHF). Pilot project was conducted in six cities, and a nationwide project was scaled up to 58 city groups with 438 volunteers. Three-step model of needs assessment, fundraising, and establishment of the supply chain was undertaken. A national team was formed consisting of representatives from multiple international organizations and stakeholders. Local Volunteers were recruited and empowered in each city to conduct donation drives. The Qualitative Comparative Analysis (QCA) model was used to assess the impact of the intervention.Results48.2% of the city groups completed needs assessment and 37.9% completed their donations. Factors such as team strength more than 4, local needs assessment, regular reporting during monthly meeting, receptive local administration, donation to more than 2 health centers and donation of supplies worth &gt;= Rs 5,000 in each city (raw coverage 0.44, consistency 1) were more important contributors for success of the outcome. Supplies worth INR 2.45 million were donated.ConclusionHyperlocal projects can effectively address essential supply shortages. A three-step model of needs assessment, fundraising, and supply chain establishment can be an effective approach. Community involvement and donations are crucial for the success and sustainability of such projects.
An investigation of the risk factors of chronic obstructive pulmonary disease in natural population-based cohorts in China – a nested case-control study
Authors: Xu Yixin , Zhao Hongjun , Yu Chunchun , Wang Yuqian , Xu Hao , Weng Zhe , Chen Chengshui , Mao Haizhou 
Published: December 2023

BackgroundChronic obstructive pulmonary disease (COPD) has become one of the most significant chronic diseases in China. According to conventional wisdom, smoking is the pathogenic factor. However, current research indicates that the pathophysiology of COPD may be associated with prior respiratory system events (e.g., childhood hospitalization for pneumonia, chronic bronchitis) and environmental exposure (e.g., dust from workplace, indoor combustion particles). Dyspnea, persistent wheezing, and other respiratory symptoms further point to the need for pulmonary function tests in this population. Reducing the burden of chronic diseases in China requires a thorough understanding of the various factors that influence the occurrence of COPD.MethodsUsing a cohort from the natural population, this study used nested case-control analysis. We carried out a number of researches, including questionnaire surveys and pulmonary function testing, in the Northwest and Southeast cohorts of China between 2014 and 2021. After removing any variations in the baseline data between patients and control subjects using propensity score matching analysis, the risk factors were examined using univariate or multivariate regression.ResultIt was discovered that prior history of chronic bronchitis, long-term wheezing symptoms, and environmental exposure—including smoking and biofuel combustion—were risk factors for COPD. Dyspnea, symptoms of mobility limitation, organic matter, and a history of hospitalization for pneumonia at an early age were not significant in the clinical model but their incidence in COPD group is higher than that in healthy population.DiscussionCOPD screening effectiveness can be increased by looking for individuals with chronic respiratory symptoms. Smokers should give up as soon as they can, and families that have been exposed to biofuels for a long time should convert to clean energy or upgrade their ventilation. Individuals who have previously been diagnosed with emphysema and chronic bronchitis ought to be extra mindful of the prevention or advancement of COPD.
Long-term burden of war injuries among civilians in LMICs: case of the July 2006 war in Lebanon
Authors: Kobeissi Elsa , Menassa Marilyne , Honein-AbouHaidar Gladys , El Achi Nassim , Abdul-Sater Zahi , Farhat Theresa , Al Mohtar Dalia , Hajjar Marwan , Abdul-Khalek Rima A. , Chaya Bachar F. , Elamine Ahmad , Hettiaratchy Shehan , Abu-Sittah Ghassan 
Published: December 2023

IntroductionLebanon, a country located on the eastern shore of the Mediterranean Sea, is one of the world’s smaller sovereign states. In the past few decades, Lebanon endured a perpetual political turmoil and several armed conflicts. July 12, 2006, marked the start of a one-month war in Lebanon, which resulted in thousands of casualties. Little is known about the long-term consequences of war injuries inflicted on civilians during the July 2006 war.MethodsThe objectives of this paper were to identify and evaluate: 1- civilians’ access to healthcare and medicine under conditions of war; 2- the long-term socioeconomic burden on injured civilians; and 3- their quality of life more than a decade post-war. We adopted a mixed-method research design with an emphasis on the qualitative component. We conducted interviews with patients, collected clinical and financial data from hospital medical records, and administered a self-rated health questionnaire, the EQ-5D-5L. Simple descriptive statistics were calculated using Excel. NVivo 12® was used for data management and thematic analysis.ResultsWe conducted 25 interviews. Injured civilians were mostly males, average age of 27. The most common mechanism of injury was blast injury. Most patients underwent multiple surgeries as well as revision surgeries. The thematic analysis revealed three themes: 1- recall of the time of the incident, the thousand miles journey, and patients’ access to services; 2- post-trauma sequelae and services; and 3- long-term impact. Patients described the long-term burden including chronic pain, poor mobility, anxiety or depression, and limited activities of daily living.DiscussionCivilians injured during the July 2006 war described the traumatising events they endured during the war and the limited access to medical care during and post-war. Up until this study was conducted, affected civilians were still experiencing physical, psychological, and financial sequelae. Acknowledging the limitations of this study, which include a small sample size and recall bias, the findings underscore the necessity for the expansion of services catering to civilians injured during wartime.
Use of metabolites, metabolithotropic agents and nutritional supplements in sports and sports medicine: a modern view on the problem
Authors: Bielenichev I. F. , Gorchakova N. A. , Doroshenko E. Yu. , Samura I. B. , Ryzhenko V. P. , Bukhtiiarova N. V. 
Published: December 2023

Aim. The purpose of this study was to conduct an up-to-date semantic analysis of the results of our own research and literature data on the medical and pharmacological characteristics of metabolites, metabolitotropic agents and nutritional supplements, the peculiarities of their use in sports practice and sports medicine.
Materials and methods. This study used bibliosemantic, analytical, logical, and generalization methods. The life sciences and biomedical information bibliographic databases MEDLINE, EMBASE, Medline (PubMed), Web of Science, and Cochrane Central were searched to find publications in English that matched the research keywords. The authors carried out an independent search and selection of articles, assessment of the quality of the data, compliance of the presentation and interpretation with the main idea of the study, with the formation of the final list of references.
Results. Based on the analysis of modern literature data and the results of our own studies, a generalized medical and pharmacological characteristic of metabolite agents, metabolitotropic drugs and nutritional supplements that can influence the main and compensatory mechanisms of macroergic phosphates production under the influence of physical activity is presented. The mechanisms of action, indications for use and main side effects of drugs and food additives based on ATP, malate, succinate, citrate, pyruvate, carnitine, carnosine, etc. are presented.
Conclusions. Semantic analysis data indicate that an urgent problem for sports medicine physicians and pharmacologists is the development and use of metabolites, metabolitotropic agents and nutritional supplements, as well as approaches to their rational combination to improve energy metabolism, replenish ATP reserves in the body that will help ensure the intensity muscular activity and simultaneously protect target organs.
A Novel Techniques to Reduce the Test Data Volume and Test Time for General Configurations by Embedded Deterministic Testing
Authors: K Praveen , S Dr Rajanna G , swamy G M Dr Shivakumara 
Published: December 2023

<p>The main goal of Design for Testability (DFT) is to offer a way to test each node in the design (Netlist) for different kinds of defects in the chip. These nodes that are to be checked by using the intended number of patterns, the more thoroughly the design has been tested. Every node in the architecture must be visible(observable) and controlled for this to be achievable. These two concepts may be thought of as the cornerstones of DFT, which must be adhered to in order to get the most test coverage with the fewest possible patterns. This work is applied in the field of IC manufacturing before IC gets fabricated. The parameters like multi-voltages and temperature of IC will be determined with the help of Scan Insertion, ATPG pattern generation, EDT test compression and simulation using mentor graphics tool. In this script we are mainly concentrated on the compression process of the data volume and test time by generating automatic test patterns according to it and simulating </p>