bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Relationship Marketing Terhadap Loyalitas Konsumen Klinik Navaagreen Batam
Authors: Sukati Inda , Rustam Triana Ananda 
Published: October 2023

The concept of relationship marketing emerged in the fields of services marketing and industrial marketing. Relationship marketing combines offline and online strategies to strengthen bonds between businesses and customers. In essence, it involves investing heavily in connections with customer.. The objective of this study is to investigate how he trust, commitment, communication, and conflict handling influence the customer loyalty. The data collection instrument used was a questionnaire which was administrated to a total sample of 100 consumers of Navaagreen Batam Clinic. The analyses involved statistical methods such reliability and validity tests and multiple regression. The results indicated that there are positive significant relationship between (a) trust, (b) commitment, (c) communication and consumer loyalty at Navaagreen Batam Clinic. The finding also indicated that there is negative insignificant relationship between Handling Customer Complaints/Conflicts and consumer loyalty at Navaagreen Batam Clinic. The study therefore recommends that Navaagreen Batam Clinic need to implement the strategy to maintain customer trust, commitment and communication. The current study also suggest to Navaagreen Batam Clinic for improving the customer’s complaint handling system as well as to show more care and concern when relating to their customers as all these give the consumers a seen as assurance and honor as revealed by the study result.
Analisis Penerapan Akuntansi Biaya Bahan Baku Dan Biaya Tenaga Kerja Langsung Terhadap Peningkatan Hasil Produksi Pada PT Ghim Li Indonesia
Authors: Harahap Baru , Laili Nur Isra , Rahmat Muhammad 
Published: October 2023

The purpose of this study was to find out whether the cost of raw materials and direct labor costs had an effect on the increase in production. In this quantitative research two types of variables are used, namely the independent variable is Raw Material Cost, Direct Labor Cost and the dependent variable is Increased Production Results. Sampling is done by the technique used, namely purposive sampling. Data analysis using Classical Assumptions and Multiple Linear Regression methods. The results of the study were processed using SPSS 27, it can be seen that the Raw Material Cost has a tcount of 4.616 with a significance probability of 0.000, concluded that Raw Material Costs partially have a positive and significant effect on Increasing Production Cost of Direct Labor Costs has t count of 4.944 with a significance of 0,000 can be concluded that Direct Labor Costs partially have a positive and significant effect on Increasing Production Results. The F test shows the Fcount value of 71,270 and significance of 0,000b means that there is a significant effect between Raw Material Costs and Direct Labor Costs on Increasing Production Results.
Aplikasi Pembelajaran Kosa Kata Untuk Anak Berkebutuhan Khusus Tunarungu Menggunakan Metode Algoritma Fisher-Yates
Authors: Saputra Aggry , Zulfachmi Zulfachmi , Alviandi Agung 
Published: October 2023

Deafness is a type of special need that is of concern to the government in terms of equality in obtaining education as stated in Law of the Republic of Indonesia No. 20 of 2003 concerning National Education Chapter IV Article 5 paragraph (1) states that "Every citizen has the same right to obtain quality education" and paragraph (2) "Citizens who have physical, emotional, mental, intellectual and /or social rights to obtain special education”. The problem faced by deaf student is the difficulty of capturing the information conveyed by the teacher during the learning process. These limitations make it difficult for deaf children to master good and correct vocabulary. The aim of this research is to help student with special needs learn vocabulary through learning media.
With this application, it is hoped that it can meet the vocabulary learning needs, especially for Deaf SMPLB students at SLB Karya Mandiri Kijang.
Pengelolaan Layanan Sistem Kehadiran Kerja Dosen Berbasis ITIL Versi 3.0
Authors: Widiantoro Suryo , Marfuah Marfuah , Randi Randi 
Published: October 2023

Quality is something that becomes a reference in assessing something. Good performance is always associated with good quality, so that good data and information management will have an impact on good performance in order to meet the desired quality. This research aims to apply the principles of the information technology service management framework based on ITIL version 3.0 into the context of managing the development of a lecturer attendance system service within the Faculty of Computers at Universal University. Through the service design domain, this research succeeded in developing recommendations for service system development in the aspects of service catalog management, capacity management, availability management, information technology service sustainability management, and information security management.
Keywords: Service management; lecturer performance; ITSM; ITIL version
Efficacy and safety of clopidogrel versus aspirin monotherapy for secondary prevention in patients with coronary artery disease: a meta-analysis
Authors: Liu Di , Xu Wei Pan , Xu Hang , Zhao Lin , Jin Dao Qun 
Published: October 2023

BackgroundThe benefits and risks of aspirin verse clopidogrel monotherapy in patients with coronary artery disease (CAD) remain controversial. This meta-analysis evaluated the efficacy and safety of aspirin verse clopidogrel monotherapy for long-term treatment in patients with CAD.MethodsLiterature was searched in the Pubmed, the Cochrane Library, and the Embase databases until March 2023. The Cochrane Risk of Bias Tool was used to assess the risk of bias in included studies. Data were extracted from the included studies, heterogeneity analysis, and pooled analysis conducted by RevMan 5.3 software.ResultsA total of five trials were included, involving 11, 766 patients with CAD. Compared with the aspirin group, the clopidogrel group was associated with reduced risk of major adverse cardiac and cerebrovascular events (MACCE) [risk ratio (RR) = 0.68, P = 0.0007], myocardial infarction (MI, RR = 0.66, P = 0.01), stroke (RR = 0.58, P = 0.003), and BARC major bleeding (RR = 0.63, P = 0.02). There were no significant differences in death from any cause (RR = 1.06, P = 0.59) and vascular death (RR = 0.92, P = 0.62) between the two groups.ConclusionsPatients with CAD use clopidogrel could further reduce the risk of MACCE, MI, stroke, and BARC major bleeding, compared with the use of aspirin. This finding supported the use of clopidogrel rather than aspirin in patients with CAD who required long-term antiplatelet monotherapy for preventing ischemic events.
Real-world utilization of guideline-directed genetic testing in inherited cardiovascular diseases
Authors: Longoni Mauro , Bhasin Kanchan , Ward Andrew , Lee Donghyun , Nisson McKenna , Bhatt Sucheta , Rodriguez Fatima , Dash Rajesh 
Published: October 2023

BackgroundCardiovascular disease continues to be the leading cause of death globally. Clinical practice guidelines aimed at improving disease management and positively impacting major cardiac adverse events recommend genetic testing for inherited cardiovascular conditions such as dilated cardiomyopathy (DCM), hypertrophic cardiomyopathy (HCM), long QT syndrome (LQTS), hereditary amyloidosis, and familial hypercholesterolemia (FH); however, little is known about how consistently practitioners order genetic testing for these conditions in routine clinical practice. This study aimed to assess the adoption of guideline-directed genetic testing for patients diagnosed with DCM, HCM, LQTS, hereditary amyloidosis, or FH.MethodsThis retrospective cohort study captured real-world evidence of genetic testing from ICD-9-CM and ICD-10-CM codes, procedure codes, and structured text fields of de-identified patient records in the Veradigm Health Insights Ambulatory EHR Research Database linked with insurance claims data. Data analysis was conducted using an automated electronic health record analysis engine. Patient records in the Veradigm database were sourced from more than 250,000 clinicians serving over 170 million patients in outpatient primary care and specialty practice settings in the United States and linked insurance claims data from public and private insurance providers. The primary outcome measure was evidence of genetic testing within six months of condition diagnosis.ResultsBetween January 1, 2017, and December 31, 2021, 224,641 patients were newly diagnosed with DCM, HCM, LQTS, hereditary amyloidosis, or FH and included in this study. Substantial genetic testing care gaps were identified. Only a small percentage of patients newly diagnosed with DCM (827/101,919; 0.8%), HCM (253/15,507; 1.6%), LQTS (650/56,539; 1.2%), hereditary amyloidosis (62/1,026; 6.0%), or FH (718/49,650; 1.5%) received genetic testing.ConclusionsGenetic testing is underutilized across multiple inherited cardiovascular conditions. This real-world data analysis provides insights into the delivery of genomic healthcare in the United States and suggests genetic testing guidelines are rarely followed in practice.
Sex differences and estrogen effects in cardiac mitochondria in human aortic stenosis and in the mouse heart
Authors: Fliegner Daniela , Ellieva Alexandra , Angelov Anja , Petrov Georgi , Regitz-Zagrosek Vera 
Published: October 2023

IntroductionSex differences in the adaptation to pressure overload have been described in humans, as well as animal models, and have been related to sex-specific expression of mitochondrial genes. We therefore tested whether sex differences in cardiac mitochondrial respiration exist in humans with aortic stenosis (AS). We also examined whether these potential differences may be at least partially due to sex hormones by testing if mitochondrial respiration is affected by estrogen (17ß-estradiol (E2)).MethodsConsecutive patients undergoing transapical aortic valve implantation (TAVI) (women, n = 7; men, n = 10) were included. Cardiac biopsies were obtained during TAVI and used directly for mitochondrial function measurements. Male and female C57BL/6J mice (n = 8/group) underwent sham surgery or gonadectomy (GDX) at the age of 2 months. After 14 days, mice were treated once with intraperitoneally injected vehicle (placebo), 17ß-estradiol (E2), estrogen receptor alpha (ERα) agonist [propyl pyrazole triol (PPT)], or ER beta (ERβ) agonist (BAY-1214257). Thereafter, mitochondrial measurements were performed directly in cardiac skinned fibers from isolated left ventricles and musculus solei.ResultsMitochondrial State-3 respiration was higher in female than that in male human heart biopsies (15.0 ± 2.30 vs. 10.3 ± 2.05 nmol/mL/min/mg, p&lt; 0.05). In the mouse model, mitochondrial State-3 respiration decreased significantly after GDX in female (27.6 ± 1.55 vs. 21.4 ± 1.71 nmol/mL/min/mg; p&lt; 0.05) and male hearts (30.7 ± 1,48 vs. 23.7 ± 2,23 nmol/mL/min/mg; p&lt; 0.05). In ovariectomized female mice, E2 and ERβ-agonist treatment restored the State-3 respiration to intact placebo level, whereas ERα-agonist treatment did not modulate State-3 respiration. The treatment with E2, ERα-, or ERβ-agonist did not modulate the State-3 respiration in GDX male mice.ConclusionWe identified sex differences in mitochondrial respiration in the diseased human heart. This is in alignment with known sex differences in the gene expression and proteome level at the functional level. E2 and ERβ affect cardiac mitochondrial function in the mouse model, suggesting that they may also contribute to the sex differences in the human heart. Their roles should be further investigated.
Expanded population of low-density neutrophils in juvenile idiopathic arthritis
Authors: Parackova Zuzana , Zentsova Irena , Bloomfield Marketa , Klocperk Adam , Horvath Rudolf , Malcova Hana , Cebecauerova Dita , Sediva Anna 
Published: October 2023

IntroductionJuvenile idiopathic arthritis (JIA), a clinically variable disease characterized by autoimmune arthritis, affects children, and its immunopathology remains elusive. Alterations in neutrophil biology play an important role in this disease. In the present study, we aimed to explore the features of low-density neutrophils (LDNs) in patients with JIA.MethodsGene expression of peripheral blood mononuclear cells (PBMCs) from children with distinct subtypes of JIA was analyzed by NanoString Immunology panel. Presence of LDNs was ascertained by flow cytometry and the release of neutrophil-associated products were analyzed by LUMINEX.ResultsLDNs were detected in patients’ peripheral blood mononuclear cells (PBMCs) after density gradient centrifugation. Transcriptomic analysis of JIA PBMCs revealed that genes related to neutrophil degranulation were markedly upregulated. The number of LDNs and level of their degranulation products increased in patients’ PBMCs and correlated with serum calprotectin, but not with disease activity, sedimentation rate and C-reactive protein (CRP) levels. The phenotypes of LDNs varied from those of normal-density neutrophils and healthy donor LDNs. Phenotypical analysis revealed LDNs are immature and primed population with decreased suppressive capacity. A negative correlation between surface proteins CD62L, CD66b, and CD11b and the number of inflamed joints/JADAS was established.ConclusionOur results describe LDNs as primed, degranulated, immature cells with impaired suppressive activities. This work thus contributes to the increasing body of evidence that LDNs in JIA are altered and their role in the disease immunopathogenesis and possible clinical associations should be investigated further.
Clinical outcomes and timing on the combination of focal radiation therapy and immunotherapy for the treatment of brain metastases
Authors: Antelo Gabriela , Comas Silvia , Casas Francesc , Valduvieco Izaskun , Barreto Tanny , Laplana María , Mases Joel , Oses Gabriela , Mollà Meritxell 
Published: October 2023

IntroductionRadiotherapy is one of the standard treatments for brain metastases (BM). Over the past years, the introduction of immunotherapy as routine treatment for solid tumors has forced investigators to review and evaluate how it would interact with radiation. Radiation and Immunotherapy have shown a synergic effect activating the host’s immune system and enhancing treatment response. The combinatory effect on BM is currently under investigation.MethodsData published on Pubmed to determine toxicity, survival, treatment characteristics and timing on the combination of radiotherapy and immunotherapy for the treatment of BM has been reviewed.ResultsMostly retrospective reviews report an improvement of intracranial progression free survival (iPFS) when combining radioimmunotherapy for BM patients. Two systematic reviews and meta-analysis and one phase II prospective trial also report a benefit on iPFS without an increase of toxicity. Among the published literature, the definition of concurrency is heterogeneous, being one month or even narrowed intervals correlated to better clinical outcomes. Toxicity due to concurrent radioimmunotherapy, specifically symptomatic radionecrosis, is also directly analyzed and reported to be low, similar to the toxicity rates secondary to stereotactic radiosurgery alone.ConclusionRadiation combined with immunotherapy has shown in predominantly retrospective reviews a synergic effect on the treatment of BM. The concurrent combination of radioimmunotherapy is a feasible therapeutic strategy and seems to improve clinical outcomes, especially iPFS, when delivered within &lt;30 days. Larger prospective and randomized studies are needed to establish reliable outcomes, best delivery strategies and toxicity profile.
Automated EuroFlow approach for standardized in-depth dissection of human circulating B-cells and plasma cells
Authors: Delgado Alejandro H. , Fluxa Rafael , Perez-Andres Martin , Diks Annieck M. , van Gaans-van den Brink Jacqueline A. M. , Barkoff Alex-Mikael , Blanco Elena , Torres-Valle Alba , Berkowska Magdalena A. , Grigore Georgiana , van Dongen J .J .M. , Orfao Alberto 
Published: October 2023

BackgroundMultiparameter flow cytometry (FC) immunophenotyping is a key tool for detailed identification and characterization of human blood leucocytes, including B-lymphocytes and plasma cells (PC). However, currently used conventional data analysis strategies require extensive expertise, are time consuming, and show limited reproducibility.ObjectiveHere, we designed, constructed and validated an automated database-guided gating and identification (AGI) approach for fast and standardized in-depth dissection of B-lymphocyte and PC populations in human blood.MethodsFor this purpose, 213 FC standard (FCS) datafiles corresponding to umbilical cord and peripheral blood samples from healthy and patient volunteers, stained with the 14-color 18-antibody EuroFlow BIgH-IMM panel, were used.ResultsThe BIgH-IMM antibody panel allowed identification of 117 different B-lymphocyte and PC subsets. Samples from 36 healthy donors were stained and 14 of the datafiles that fulfilled strict inclusion criteria were analysed by an expert flow cytometrist to build the EuroFlow BIgH-IMM database. Data contained in the datafiles was then merged into a reference database that was uploaded in the Infinicyt software (Cytognos, Salamanca, Spain). Subsequently, we compared the results of manual gating (MG) with the performance of two classification algorithms -hierarchical algorithm vs two-step algorithm- for AGI of the cell populations present in 5 randomly selected FCS datafiles. The hierarchical AGI algorithm showed higher correlation values vs conventional MG (r2 of 0.94 vs. 0.88 for the two-step AGI algorithm) and was further validated in a set of 177 FCS datafiles against conventional expert-based MG. For virtually all identifiable cell populations a highly significant correlation was observed between the two approaches (r2&gt;0.81 for 79% of all B-cell populations identified), with a significantly lower median time of analysis per sample (6 vs. 40 min, p=0.001) for the AGI tool vs. MG, respectively and both intra-sample (median CV of 1.7% vs. 10.4% by MG, p&lt;0.001) and inter-expert (median CV of 3.9% vs. 17.3% by MG by 2 experts, p&lt;0.001) variability.ConclusionOur results show that compared to conventional FC data analysis strategies, the here proposed AGI tool is a faster, more robust, reproducible, and standardized approach for in-depth analysis of B-lymphocyte and PC subsets circulating in human blood.
Longbie capsules reduce bone loss in the subchondral bone of rats with comorbid osteoporosis and osteoarthritis by regulating metabolite alterations
Authors: Liang Guihong , Zhao Jinlong , Zhao Di , Dou Yaoxing , Huang Hetao , Yang Weiyi , Zhou Guanghui , Gu Zhuoxu , Pan Jianke , Liu Jun 
Published: October 2023

Background and objectiveWith the development of global population aging, comorbidity (≥2 diseases) is a common health problem among elderly people. Osteoarthritis (OA) and osteoporosis (OP) are common in elderly individuals. There is a lack of drug therapy for OA and OP comorbidities. The purpose of this study was to explore the efficacy and mechanism of Longbie capsule (LBJN), which contains various plant herbs, in treating OA and OP comorbidities (OA + OP) in rats using metabolomics techniques.MethodsWe created an OA + OP rat model through bilateral oophorectomy combined with meniscus instability surgery. Thirty SD rats were randomly divided into five groups (six in each group), namely, the sham group, OA group, OA + OP group, LBJN low-dose group (0.625 g/kg, OA + OP+LB-L group) and LBJN high-dose group (1.25 g/kg, OA + OP+LB-H group). After 8 weeks of intervention, we used micro-CT to detect bone microstructure status, ELISA to measure bone metabolism indicators, and UPLC–MS technology for metabolomics analysis. Finally, the screened differentially expressed metabolites were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and functional enrichment analysis.ResultsThe micro-CT results showed that LBJN significantly improved the bone mineral density (BMD) and bone quality of subchondral bone in OA + OP rats, and LBJN regulated the expression of bone alkaline phosphatase (BALP), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRACP) in serum to maintain bone metabolism balance. Metabolomics analysis showed that the metabolic trajectory of OA + OP rats after intervention in the OA + OP+LB-H group showed significant changes, and 107 potential biomarkers could be identified. Among them, 50 metabolites were upregulated (such as zeranol) and 57 were downregulated (such as vanillactic acid). The KEGG functional enrichment results indicated that the differentially expressed metabolites are mainly involved in amino acid metabolism, lipid metabolism, and carbohydrate metabolism. The KEGG pathway enrichment results indicated that LBJN may exert therapeutic effects on OA + OP rats by regulating the cAMP signaling pathway, and the FoxO signaling pathway.ConclusionLBJN can maintain bone metabolism balance by regulating serum lipid metabolism, amino acid metabolism, carbohydrate metabolism, and estrogen, thereby reducing bone loss in subchondral bone, which may be a potential mechanism through which LBJN treats OA + OP.
An autonomous mobile robot path planning strategy using an enhanced slime mold algorithm
Authors: Zheng Ling , Hong Chengzhi , Song Huashan , Chen Rong 
Published: October 2023

IntroductionAutonomous mobile robot encompasses modules such as perception, path planning, decision-making, and control. Among these modules, path planning serves as a prerequisite for mobile robots to accomplish tasks. Enhancing path planning capability of mobile robots can effectively save costs, reduce energy consumption, and improve work efficiency. The primary slime mold algorithm (SMA) exhibits characteristics such as a reduced number of parameters, strong robustness, and a relatively high level of exploratory ability. SMA performs well in path planning for mobile robots. However, it is prone to local optimization and lacks dynamic obstacle avoidance, making it less effective in real-world settings.MethodsThis paper presents an enhanced SMA (ESMA) path-planning algorithm for mobile robots. The ESMA algorithm incorporates adaptive techniques to enhance global search capabilities and integrates an artificial potential field to improve dynamic obstacle avoidance.Results and discussionCompared to the SMA algorithm, the SMA-AGDE algorithm, which combines the Adaptive Guided Differential Evolution algorithm, and the Lévy Flight-Rotation SMA (LRSMA) algorithm, resulted in an average reduction in the minimum path length of (3.92%, 8.93%, 2.73%), along with corresponding reductions in path minimum values and processing times. Experiments show ESMA can find shortest collision-free paths for mobile robots in both static and dynamic environments.