bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telesupervision in genetic counseling education during COVID‐19 and beyond
Authors: Kessler Lisa Jay , Mundt Michaela , Freiberg Yael , Taylor Lynne , Valverde Kathleen D. 
Published: January 2024

AbstractThe COVID‐19 pandemic led healthcare organizations to pivot to telemedicine, precipitating the shift to telesupervision, defined as supervision over video‐conferencing platforms and telephone, for genetic counseling students. This study aimed to (1) characterize the impact of the COVID‐19 pandemic on genetic counseling supervision, (2) ascertain genetic counseling supervisor experiences with telesupervision, and (3) examine the association between years of genetic counseling experience and preferences related to supervision modality. Certified genetic counselors were recruited through the National Society of Genetic Counselors (NSGC), Pennsylvania Association of Genetic Counselors (PAGC), and Association of Genetic Counseling Program Directors (AGCPD) listservs and by purposive sampling via emails to Genetic Counseling Program Directors in program‐rich regions. An investigator‐created survey was administered online to elicit descriptions of supervision during the pandemic, including modifications to rotations, changes to the number of students supervised, and mode of supervision preference. The survey was completed by 122 genetic counselors. The 54‐question survey included multiple‐choice, ranking, and open‐ended questions. The majority (95.9%) of the participants were female, with an average age of 32 years. Most (86%) of participants reported changes in their supervision practices due to COVID‐19, including sole use of telesupervision (54.4%), decreases in rotation lengths (25.4%), and delays in rotation start dates (24.6%). Interestingly, 19.3% (22/114) supervised more students than before the pandemic, and 18.4% (21/114) of participants supervised fewer students. Approximately two thirds (67.5%) of supervisors preferred supervising students in‐person, while 2.6% preferred telesupervision, and 29.8% had no preference. Those who preferred to supervise in‐person had fewer years of experience than those who had no preference (median = 4 years vs. median = 7 years respectively, Wilcoxonp‐value = 0.0418, effect size rpb = 0.19, small). Given the persistence of telemedicine beyond the pandemic, telesupervision may be necessary to train genetic counseling students. However, additional research is needed to determine the effectiveness of telesupervision in facilitating student skill development and attainment of the Accreditation Council for Genetic Counseling (ACGC) Practice‐Based Competencies (PBCs).
Repurposing empagliflozin in individuals with glycogen storage disease Ib: A value‐based healthcare approach and systematic benefit‐risk assessment
Authors: Derks Terry G. J. , Venema Annieke , Köller Clara , Bos Eline , Overduin Ruben J. , Stolwijk Nina N. , Hofbauer Peter , Bolhuis Mathieu S. , van Eenennaam Fred , Groen Henk , Hollak Carla E. M. , Wortmann Saskia B. 
Published: January 2024

AbstractOff‐label repurposing of empagliflozin allows pathomechanism‐based treatment of neutropenia/neutrophil‐dysfunction in glycogen storage disease type Ib (GSDIb). From a value‐based healthcare (VBHC) perspective, we here retrospectively studied patient‐reported, clinical and pharmacoeconomic outcomes in 11 GSDIb individuals before and under empagliflozin at two centers (the Netherlands [NL], Austria [AT]), including a budget impact analysis, sensitivity‐analysis, and systematic benefit–risk assessment. Under empagliflozin, all GSDIb individuals reported improved quality‐of‐life‐scores. Neutrophil dysfunction related symptoms allowed either granulocyte colony‐stimulating factor cessation or tapering. Calculated cost savings per patient per year ranged between € 6482–14 190 (NL) and € 1281–41 231 (AT). The budget impact analysis estimated annual total cost savings ranging between € 75 062–225 716 (NL) and € 37 697–231 790 (AT), based on conservative assumptions. The systematic benefit‐risk assessment was favorable. From a VBHC perspective, empagliflozin treatment in GSDIb improved personal and clinical outcomes while saving costs, thereby creating value at multiple pillars. We emphasize the importance to reimburse empagliflozin for GSDIb individuals, further supported by the favorable systematic benefit‐risk assessment. These observations in similar directions in two countries/health care systems strongly suggest that our findings can be extrapolated to other geographical areas and health care systems.
Avoidance and escape conditioning adjust adult neurogenesis to conserve a fit hippocampus in adult male rodents
Authors: Mohammadi Masoud , Tavassoli Zohreh , Anvari Sohrab , Javan Mohammad , Fathollahi Yaghoub 
Published: January 2024

AbstractIn this study, the connection between cognitive behaviors and the adult rodent hippocampus was investigated. Recording field potentials at performant pathway (PP)–hippocampal dentate gyrus (DG) synapses in transverse slices from the dorsal (d), intermediate (i), and ventral (v) hippocampus showed differences in paired‐pulse responses and long‐term potentiation in rats. The Barnes maze (BM) and passive avoidance (PA) tests indicated a decrease in escape latency and step‐through latency in both rats and mice over training days. A decrease in the use of random or sequential strategy while an increase in the use of direct strategy to search for an escape box occurred in both groups. Evaluation of the levels of neurogenesis markers (Ki67 and BrdU/NeuN) by immunofluorescence assay in the dDG, iDG, and vDG revealed a long‐axis disparity in the hippocampal dentate baseline cell proliferation and exposure to the BM and PA task changed the profile of baseline cell proliferation along the DG in both rats and mice. Also, these learning experiences changed the profile of BrdU+/NeuN+ cells along the DG of rats. Quantitation of hippocampal BDNF protein levels using ELISA exhibited no changes in BDNF levels due to learning experiences in rats. We demonstrate that PP–DG synaptic efficacy and neurogenesis are organized along a gradient. Avoidance and escape conditioning themselves are sufficient to change and calibrate adult neurogenesis along the hippocampal long axis in rodents. Further research will be required to determine the precise mechanisms underlying the role of experience‐derived neuroplasticity in cognitive function and decline.
Efficacy of infliximab after loss of response of/intolerance to adalimumab in pediatric Crohn's disease: A retrospective multicenter cohort study of the “GETAID pédiatrique”
Authors: Lecoutour Anne , Dupont Claire , Caldari Dominique , Dumant Clémentine , Vanrenterghem Audrey , Ruiz Mathias , Duclaux‐Loras Rémi , Berthet Stéphanie , Dimitrov Georges , Lacroix Delphine , Duvant Pauline , Roman Céline , Wagner Anne Claire , Bourmaud Aurélie , Viala Jérôme , Ruemmele Frank M. , Pigneur Bénédicte ,   the GETAID pédiatrique group
Published: January 2024

AbstractBackgroundInfliximab (IFX) and adalimumab (ADA) are recommended for induction and maintenance of remission in pediatric Crohn's disease (CD). ADA is now often used in first line due to its efficacy and tolerability, but a loss of response (LOR) can occur over time. The aim was to assess the efficacy of IFX as second line therapy after LOR or intolerance to ADA in pediatric CD patients at 1 year.MethodsWe conducted a retrospective and multicenter study in France among the “GETAID pédiatrique” centers between April 2019 and April 2022. CD patients under 18 years old and treated with IFX after ADA failure or intolerance were included. We collected anthropometric, clinical, and biological data at baseline (start of IFX), at 6 and 12 months. Clinical remission was defined by a Weighted Pediatric CD Activity Index (wPCDAI) score less than 12.5 points.ResultsOf the 32 patients included in our study, 27 (84.4%) were still on IFX at 12 months of the switch. Among them, 13 had discontinued ADA because of a LOR, 12 for insufficient response and 2 due to primary nonresponse. At M12, 22 patients were in corticosteroid free clinical remission (68.7%). Under IFX, the wPCDAI decreased over time (47.5 ± 24.1, 16.6 ± 21.2 and 9.7 ± 19.0 at M0, M6 and M12 respectively). The only factor associated with clinical remission at 12 months was absence of perianal disease at the end of the IFX induction.ConclusionsIFX is effective in maintaining remission at 1 year in pediatric CD patients experiencing a LOR or intolerance with ADA, and IFX could be an interesting therapeutic choice instead of other biologics in this situation.
Enhancing recall in automated record screening: A resampling algorithm
Authors: Hou Zhipeng , Tipton Elizabeth 
Published: January 2024

AbstractLiterature screening is the process of identifying all relevant records from a pool of candidate paper records in systematic review, meta‐analysis, and other research synthesis tasks. This process is time consuming, expensive, and prone to human error. Screening prioritization methods attempt to help reviewers identify most relevant records while only screening a proportion of candidate records with high priority. In previous studies, screening prioritization is often referred to as automatic literature screening or automatic literature identification. Numerous screening prioritization methods have been proposed in recent years. However, there is a lack of screening prioritization methods with reliable performance. Our objective is to develop a screening prioritization algorithm with reliable performance for practical use, for example, an algorithm that guarantees an 80% chance of identifying at least of the relevant records. Based on a target‐based method proposed in Cormack and Grossman, we propose a screening prioritization algorithm using sampling with replacement. The algorithm is a wrapper algorithm that can work with any current screening prioritization algorithm to guarantee the performance. We prove, with mathematics and probability theory, that the algorithm guarantees the performance. We also run numeric experiments to test the performance of our algorithm when applied in practice. The numeric experiment results show this algorithm achieve reliable performance under different circumstances. The proposed screening prioritization algorithm can be reliably used in real world research synthesis tasks.
Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation
Authors: Zafar Asma , Rahman Ziaur , Hamid Attia , Sughra Fatima , Makhdoom Madood , Fatima Samreen , Ahmed Haseeb , Mehmood Zeenat , Khan Maryam , Aftab Muhammad Nauman 
Published: January 2024

AbstractPresent study concerned with expression and biochemical characterization of lipase enzyme for potential use in detergent formulation. Lipase gene (1242 bp) of Bacillus thuringensis was cloned and expressed in Escherichia coli BL21 strain using pET‐21a(+) expression vector. Maximum expression of cloned lipase gene was obtained at 37°C with an induction of 0.4 mM IPTG (Isopropyl ß‐D‐1‐thiogalactopyranoside) after 4 h of induction. Recombinant lipase was purified to homogeneity using immobilized metal ion affinity chromatography carrying 109.80 U/mg specific activity with 38.79 purification folds. Molecular mass of purified lipase was determined as 45 kDa using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Purified recombinant lipase showed stability up to 90°C and retained significant activity (52%) after 4 h at 90°C. It was also found to be stable at a wide range of pH and in the presence of higher concentrations of several inhibitors (sodium dodecyl sulfate, dimethylsulfoxide, sodium azide, β‐mercaptoethanol, polysorbate 80, dithiothreitol) as well as organic solvents (acetone, methanol, ethanol, isopropanol, n‐butanol). The activity of recombinant lipase was enhanced in the presence of various metal ions and activated up to 200% by Ca2+. The compatibility of recombinant lipase with commercial detergents and other additives as well as its broad substrate specificity endorse the potential application of this enzyme in detergent formulations.
Ultrasound Features of Nail Lichen Planus
Authors: Turner Valery L. , Wortsman Ximena 
Published: January 2024

ObjectivesTo describe the ultrasonographic features of nail lichen planus (NLP), which, so far, have not been reported in the literature.MethodsA retrospective single‐center study of NLP patients' color Doppler ultrasound examinations was performed between March 2014 and January 2023.Inclusion criteria were patients ≥15 years with a confirmed clinical diagnosis of NLP in sequential order. Exclusion criteria included concomitant systemic or local cutaneous or nail diseases and systemic or local nail treatments before the ultrasound examination. The ultrasound protocol included the examination of all fingernails or toenails following the reported protocol for dermatologic ultrasound examinations at high and ultra‐high frequencies.Patient demographics and ultrasound features of the nail bed, periungual region, nail plate, and regional vascularity were registered and analyzed statistically.ResultsA total of 36 patients met the criteria. All cases presented thickened and decreased echogenicity of the nail bed. A hypoechoic halo surrounding the origin of the nail plate was present in 78% of cases. Exactly 58% of patients presented decreased echogenicity of the proximal periungual dermis, and 86% showed thickening of the periungual dermis.Hypervascularity of the nail bed was seen in 94% of cases. The mean maximum thickness of vessels, the peak systolic velocity of the arterial vessels, and other blood flow findings are provided.ConclusionUltrasonography can support the diagnosis of NLP, which benefits the non‐invasive discrimination of nail conditions and can avoid the potential permanent scars derived from nail biopsies. Furthermore, ultrasound may be a powerful tool to monitor this disease's treatment.
Physiological and interspecific factors determine diel changes in phytoplankton bio‐optical properties
Authors: Baetge Nicholas , Halsey Kimberly H. , Graff Jason R. , Ver Wey Brian , Westberry Toby K. , Appel Amanda E. , Bourdin Guillaume , Demeaux Charlotte Begouen , Boss Emmanuel , Behrenfeld Michael J. 
Published: January 2024

AbstractBio‐optical properties of marine phytoplankton retrieved through satellite remote sensing are used to estimate ocean productivity and carbon cycling. Daily activity of phytoplankton is attuned to the predictable light fluctuations of the diel cycle. Field and laboratory studies have documented diel changes in phytoplankton growth, division, and respiration, carbon and pigment content, cell size, photosynthetic efficiency and rate, and DNA replication and transcription. Many of these physiological changes can alter cellular optical properties and contribute to diel variations in bulk absorption and scattering properties. Consequently, understanding phytoplankton contributions to diel optical cycles is essential for improving algorithms that convert remote sensing data to biological rates and stocks. Here, we describe time‐resolved cellular, photophysiological, and bio‐optical properties for three cultured phytoplankton ranging in cell size from ~ 1 to 6 μm: Ostreococcus lucimarinus, Synechococcus (WH8102), and Thalassiosira pseudonana, all of which can significantly contribute to phytoplankton abundance and/or biomass in the open and coastal ocean. This work is the first to characterize complete diel cycles in absorption and attenuation for O. lucimarinus and backscattering for all these species. Results show that the percent increase from the minimum to maximum values over the diel cycle ranged between ~ 24–121%, ~ 31–235%, and ~ 25–198% for particulate absorption, attenuation, and backscattering, respectively. Diel changes in bio‐optical properties also differed in both timing and magnitude across phytoplankton species, demonstrating the importance of contextualizing remote sensing observations with phytoplankton community composition.
High‐Speed Multi‐Modal Extended Depth‐of‐Field Microscopy with an Electrically Tunable Lens
Authors: Zhang Runnan , Zhou Ning , Tang Hanci , Xia Minhao , Cai Zewei , Sun Jiasong , Chen Qian , Zuo Chao 
Published: January 2024

AbstractHigh spatial resolution imaging over the entire volume for intravital imaging of biological specimens has long been vital. However, the depth‐of‐field (DOF) and spatial resolution are intrinsically interdependent. Here, a new extended DOF (EDOF) microscopy technique based on partially coherent annular illumination is proposed, termed AI‐EDOF, by combining an electrically tunable lens with a conventional microscope with modified illumination, for motion‐free, high spatial resolution real‐time all‐in‐focus imaging over thick volumes. EDOF imaging is obtained with spatial resolution up to near the incoherent diffraction limit (388 nm, 20/ 0.8NA) and temporal resolution 30 fps. Moreover, it is demonstrated that the coupled phase and absorption components of the complex refractive index in optical diffraction tomography microscopy can be solved by focus scanning. The Richardson‐Lucy deconvolution with total variation regularization is adopted for deblurring and suppressing noise under low light efficiency high‐speed exposure. To demonstrate the capabilities of the proposed method, experiments are conducted using fixed transgenic zebrafish larvae, Drosophila larvae and dynamic Caenorhabditis elegans under both transmissive and fluorescent imaging modalities, revealing the proposed approach is prospective to be adopted by broad applications such as pharmacokinetics and tumor immunology.
Engineering of Molecular Bridge on Buried Interface via Ferrocene Carboxylic Acid for High Performance Perovskite Light‐Emitting Devices
Authors: Xu Zehua , Feng Ting , Gao Yanbo , Zhang Fujun , Wu Yanjie , Xu Lin , Zhou Donglei , Liu Dali , Dong Biao , Bai Xue , Shi Zhifeng , Hu Junhua , Li Ting , Yan Fengping , Song Hongwei , Zhang Yu 
Published: January 2024

AbstractQuasi‐two‐dimensional (Quasi2D) perovskite materials gained widespread attention due to whose unique and highly desirable luminescence properties. However, the behavior of perovskite lightemitting diodes (PeLEDs) is prejudiced by inefficient cascading energy transfer of perovskite film and unbalanced charges injection. Here, ferrocene carboxylic acid (FcAd) is employed between hole transport layer (HTL) and perovskite layer as a molecular bridge to solve the current problems of PeLEDs. Ferrocene units can bond with [PbBr6]4−, forming one‐dimensional (1D) intermediate phases of FcPbBr3 at the interface, which can manipulate the growth kinetics of perovskite and reconstruct the phase distribution. Therefore, due to the suppression of low dimensional phase content, not only is the cascaded energy transfer of PEA2(CsPbBr3)2PbBr4 films effectively achieved, but also the quasi‐2D perovskite's work function is reduced. Additionally, Pb2+ coordinated with the carboxyl group of FcAd, inducing an electric dipole effect that leads to an further upward shift of the perovskite energy level. Eventually, the synergy achieves a significant tailoring between the perovskite and HTL energy exhibits the most excellent external quantum efficiency (EQE) exceeds 27% and optimal brightness exceeds 240000 cd m−2. Therefore, the preparation method will provide an effective strategy to widen the color gamut of next‐generation displays.
Awakening the Dumb Site to Realize Ultra‐Broadband NIR Phosphor
Authors: Jiang Lipeng , Zhang Liangliang , Zhao Xinpeng , Jiang Xue , Gao Panpan , Su Yanjing 
Published: January 2024

AbstractNear‐infrared (NIR) light sources have gained immense popularity in recent years due to their wide range of applications in various fields, including spectroscopy and biomedical imaging. However, the limited emission bandwidth of NIR phosphors is a significant bottleneck in their development. Here, a novel strategy is reported to broaden the emission bandwidth of NIR phosphors by awakening the dumb site. Na4M3Ta(PO4)6: Cr3+ (M = Al3+, Ga3+, In3+) phosphor is synthesized, which exhibits a greatly broadened bandwidth from 134 to 232 nm. Structural and spectral analysis reveals that the NaO6 octahedral site has a severe t2g‐type distortion, making it a dumb site for Cr3+. By introducing larger In3+ at the M site, the angular distortion at the NaO6 site decreases to the normal range, enabling the luminescence of Cr3+ again. Along with the broadened bandwidth, the emission peak also redshifts from 802 to 977 nm, giving advantages in NIR spectroscopy applications. Interestingly, the awakened luminescence at the NaO6 site shows even better luminescence properties than the original M/TaO6 octahedral site. These findings reveal a novel insight into the luminescence of Cr3+ at the octahedral site, which could potentially revolutionize the design of NIR phosphors.
Research on the cavitation characteristics and structural optimisation of spiral grooved thrust bearing lubricated by liquid hydrogen
Authors: Yan Shaohang , Lai Tianwei , Qiang Mingchen , Zhao Qi , Ding Wenjing , Liu Yutao , Hou Yu 
Published: January 2024

AbstractIn liquid hydrogen (LH2) delivery, submersible centrifugal pump is an important device. Its reliability and efficiency rely on the stability of the bearings. In a high‐speed range, cavitation occurs due to the hydrodynamic effect induced pressure undulation. Compared with other cryogenic fluids, cavitation in LH2 is prone to happen due to its special properties. In this paper, a cavitation model considering thermal properties of LH2 is proposed based on Rayleigh–Plesset equation. Cavitation characteristics are studied in spiral grooved thrust bearing (SGTB) lubricated by LH2. The effect of cavitation on load capacity and friction torque are investigated. The most severely‐eroded location is predicted by the distribution of mass transfer rate. The influence of bearing structure parameters is studied in respect of cavitation, load capacity and friction torque. The analysis of variance (ANOVA) and range analysis results may provide a reference for optimising LH2 lubricated SGTB.
WRF prediction of an atmospheric river‐related precipitation event: Sensitivity to cumulus parameterization schemes
Authors: Maddah Mohammad Amin , Mostamandi Suleiman 
Published: January 2024

AbstractThe present study aimed to evaluate performance sensitivity to the Cumulus Parameterization Scheme (CPS) used for the Weather Research and Forecasting (WRF) model to predict the atmospheric river‐related precipitation (ARP) event with 206 and 57 mm, highest and area‐averaged precipitation (AAP) per 24‐h, respectively, that occurred over the central mountainous basins of Iran on 31 March 2019. For this purpose, experiments were designed using the 12 (almost all) CPSs available in WRF v4. To verify the predicted precipitation (from the inner 4‐km domain), both point‐scale and grid‐scale comparisons were performed against gauge‐ and satellite‐based observational data at three accumulation time‐scales (12‐, 18‐, and 24‐h) and in three distinct sub‐regions. All scores obtained from the different statistical metrics used, are in complete agreement with a strongly dependent performance of WRF on the CPS used. In addition, the use of Kain‐Fritsch, KF‐CuP, and Grell‐3 CPSs could provide a realistic picture of impending heavy precipitation for WRF. Contrary, the New SAS, Tiedtke, and Zhang‐McFarlane CPSs did not perform satisfactorily in predicting the ARP event. As a result, CPSs with the “momentum transport” option in their modification mechanism are unlikely to adequately simulate the conversion of incoming low‐level moisture from atmospheric river to precipitation. However, precipitation predictions are more accurate at the 24‐h accumulation time‐scale than at the 12‐ and 18‐h. Also, a dry bias in the predictions is expected as the terrain elevation and accumulation time‐scale decrease and the distance from the core of the precipitation field increases.