bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulating Dipeptidyl Peptidase 3 Modulates Systemic and Renal Hemodynamics Through Cleavage of Angiotensin Peptides
Authors: Picod Adrien , Placier Sandrine , Genest Magali , Callebert Jacques , Julian Nathan , Zalc Maxime , Assad Noma , Nordin Hugo , Santos Karine , Gaudry Stéphane , Chatziantoniou Christos , Mebazaa Alexandre , Azibani Feriel 
Published: February 2024

BACKGROUND:
High circulating DPP3 (dipeptidyl peptidase 3) has been associated with poor prognosis in critically ill patients with circulatory failure. In such situation, DPP3 could play a pathological role, putatively via an excessive angiotensin peptides cleavage. Our objective was to investigate the hemodynamics changes induced by DPP3 in mice and the relation between the observed effects and renin–angiotensin system modulation.

METHODS:
Ten-week-old male C57Bl/6J mice were subjected to intravenous injection of purified human DPP3 or an anti-DPP3 antibody (procizumab). Invasive blood pressure and renal blood flow were monitored throughout the experiments. Circulating angiotensin peptides and catecholamines were measured and receptor blocking experiment performed to investigate the underlying mechanisms.

RESULTS:

DPP3 administration significantly increased renal blood flow, while blood pressure was minimally affected. Conversely, procizumab led to significantly decreased renal blood flow. Angiotensin peptides measurement and an AT1R (angiotensin II receptor type 1) blockade experiment using valsartan demonstrated that the renovascular effect induced by DPP3 is due to reduced AT1R activation
via
decreased concentrations of circulating angiotensin II, III, and IV. Measurements of circulating catecholamines and an adrenergic receptor blockade by labetalol demonstrated a concomitant catecholamines release that explains blood pressure maintenance upon DPP3 administration.

CONCLUSIONS:
High circulating DPP3 increases renal blood flow due to reduced AT1R activation via decreased concentrations of circulating angiotensin peptides while blood pressure is maintained by concomitant endogenous catecholamines release.
Neptunus and To Løver: An Archaeological, Archival, and Dendrochronological Survey of Two Danish Prize Ships, Scuttled in the Stockholm Archipelago in 1659
Authors: Daly Aoife , Eriksson Niklas , Hansson Jim 
Published: February 2024

Abstract
We report the archaeological survey, dendrochronological analysis, and archival research concerning two shipwrecks located in shallow waters outside Vaxholm in the Stockholm Archipelago, Baltic Sea. According to oral tradition, the two wrecks are the remains of Neptunus and To Løver, two former Danish warships that were taken as prizes at the battle of Fehmarn in 1644. The Swedish navy then used the two ships until 1659, when they were reportedly scuttled as a blockage along the sea route towards Stockholm. Before being intentionally scuttled, the two ships drifted away and sank. The work presented in this article confirms the substance of the tradition and reports that substantial portions of the two ships are preserved. Moreover, the two wrecks shed light on the scuttling process and the construction of an underwater barrier.
Correlation of detrended fluctuation analysis alpha 1 with blood lactate concentration in field standardised exercise tests in sport horses
Authors: Murphy K. , Solomon C. , Stefanovski D. , Lorello O. , Robinson M. , Haughan J. , Navas de Solis C. 
Published: February 2024

Abstract
Methods to determine exercise intensity thresholds, such as the aerobic and anaerobic threshold, that separate different exercise intensities in exercising horses in the field setting or on the treadmill often require equipment, standardised conditions and/or blood sampling. Detrended fluctuation analysis (DFA) is a heart rate variability variable proposed as a non-invasive tool for exercise intensity monitoring and with a potential association with exercise intensity thresholds and fatigue. The objectives of this study were to (1) describe the first component of DFA (DFA-α1) during standardised field incremental exercise tests in sport horses in active Eventing competition and in sport horses ridden without the goal of participating in competitions, and (2) to determine if DFA-α1 is associated with blood lactate concentration. Null hypotheses of the study were that (1) DFA-α1 is not correlated with blood lactate concentration measurements and (2) the correlation between DFA-α1 and blood lactate concentration is not stronger than the one between blood lactate concentration and heart rate or blood lactate concentration and speed. An in vivo observational study was performed analysing exercising electrocardiograms obtained in 59 standardised exercise tests in the field. DFA-α1 was significantly and moderately correlated with blood lactate concentration (, ). A strong correlation was observed between blood lactate concentration and speed (, ), as well as between blood lactate concentration and heart rate (, ). DFA-α1 correlation with blood lactate concentration was moderate but weaker than the correlation of blood lactate concentration and heart rate or blood lactate concentration and speed. DFA-α1 was not a useful surrogate of blood lactate concentration in field incremental standardised tests used for sport horses in this setting.
The Case for Spirit Realism: A Reply to Fales
Authors: Van Eyghen Hans 
Published: February 2024

Abstract
In this article, I respond to some criticisms raised in Evan Fales’ review of my book The Epistemology of Spirit Beliefs that was published in this journal. The points I will address are the following: (i) Fales’ complaint about unclarity in my epistemological position, (ii) his complaint about my insufficient presentation of alternative explanations, and (iii) his complaint about my use of the terms ‘naturalism’ and ‘naturalistic explanation’.
Granulin in renal tubular epithelia is associated with interstitial inflammation and activates the TLR9-IFN-α pathway in lupus nephritis
Authors: Huang Lanting , Dai Yijun , Geng Zhenbo , He Hongyan , Hong Fuyuan 
Published: February 2024

Objective This study aimed to investigate the possible role of granulin (GRN) in activating the TLR9-IFN-α pathway in renal tubular epithelial cells (RTECs) and explore clues that RTECs regulate the micro-environment of inflammatory response in lupus nephritis (LN). Methods Renal sections from 57 LN patients and 30 non-LN patients were sampled for histological study, and GRN overexpression RTECs were applied for cytological study. Results In the histological study, GRN is highly expressed in LN RTECs with tubulointerstitial inflammation (TII) and well co-localized with TLR9. ROC analysis suggested a potential relationship between GRN expression in RTECs and therapeutic response. Moreover, IFN-α also highly expressed in LN RTECs with TII, and the intensity of IFN-α is positively correlated with the co-localization intensity of GRN and TLR9. In the cytological study, LN serum, especially serum from LN with TII, activates the expression of TLR9 in RTECs, and GRN engages the interaction of TLR9 to activate the expression of IFN-α in RTECs. While TLR9 inhibitors can suppress the expression of IFN-α in RTECs, the degree of inhibition is dose-dependent. Conclusion The expression of GRN in RTECs is associated with interstitial inflammation and therapeutic response. GRN may mediate the activation of the TLR9-IFN-α pathway in RTECs and involve in the micro-environment of inflammatory response in LN.
Immunogenicity and safety of BNT162b2 vaccination in adolescents with systemic lupus erythematosus
Authors: Piyaphanee Nuntawan , Charuvanij Sirirat , Thepveera Sutheera , Toh Zheng Quan , Licciardi Paul V. , Pattaragarn Anirut , Wongprompitak Patimaporn , Boonnak Kobporn , Pheerapanyawaranun Chatkamol , Chokephaibulkit Kulkanya 
Published: February 2024

Objectives We evaluated the immunogenicity and safety of BNT162b2 vaccination in adolescents with systemic lupus erythematosus (adoSLE) receiving either high- or low-dose immunosuppressant (High-IS and Low-IS). Methods Patients aged 12–18 years diagnosed with SLE were enrolled. High-IS was defined as >7.5 mg/day prednisolone or with other immunosuppressant, while Low-IS was defined as only ≤7.5 mg/day of prednisolone and no immunosuppressant. Two doses of BNT162b2 vaccination were given 4 weeks apart, followed by a booster (third) dose at 4–6 months later. Anti-spike receptor binding domain (anti-RBD) IgG against Wuhan, neutralising antibody (NT) against Wuhan and Omicron variants, and cellular immune response by IFN-γ-ELISpot assay were evaluated following vaccination. Adverse events (AEs) and SLE flare were monitored. Results A total of 73 participants were enrolled, 40 and 33 in the High-IS and Low-IS group, respectively. At 4 weeks following the 2nd dose, overall anti-RBD IgG seropositivity was 97.3%, with no difference between the groups ( p = .498). AdoSLE on High-IS had lower anti-RBD IgG ( p < .001), Wuhan NT ( p < .001), and IFN-γ-ELISpot ( p = .022) than those on Low-IS. A 3rd dose induced significantly higher antibody responses than after the 2nd dose ( p < .001) in both groups and established seroconversion against Omicron variants, with persistent lower antibody levels in High-IS group. SELENA-SLEDAI scores within 12 weeks after 2-dose vaccination was higher than before vaccination (3.1 vs 2.5; p < .036); however, the occurrence of disease flare by SELENA-SLEDAI flare index was not different after vaccination compared to before vaccination, consistent across groups. Non-severe AEs occurred similarly in both groups. Conclusion AdoSLE on High-IS induced lower SARS-CoV-2 vaccine immune responses than Low-IS. Vaccination can increase disease activity and requires close monitoring for disease flare.
Effects of Mineral Chinese Medicine Chloriti Lapis on Gut Microbiota of Small Intestine in PTZ-kindling Epileptic Rats
Authors: Yuan Peng , Li Fengtao , Qian Xilong , Ma Yulu , Wang Xiuxiu , Zhao Qian , Zhu Xingyu , Yan Baofei , Zhou Liu , Yang Wei , Pan Yanqiong , Dai Shilin , Fang Fang , Zhao Jin , Liu Shengjin 
Published: February 2024

Background Epilepsy is a neurological disease, and its clinical treatment presents a significant medical challenge. Nevertheless, Chloriti Lapis (CL), a traditional Chinese Mineral Medicine, has been employed for centuries to address neurological disorders and has shown notable effectiveness. Objectives The objectives of this study are to further explore whether CL can modulate the abundance and diversity of the small intestinal flora in rats with epilepsy induced by pentylenetetrazol (PTZ) and to investigate the interrelationship among the flora. Materials and Methods Operational taxonomic units (OTUs), α diversity, β diversity, hierarchical clustering tree, and principal component analysis (PCA) were utilized to analyze the small intestinal flora of PTZ-kindled epileptic rats. Results The predominant bacterial groups in the small intestine mainly belonged to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. The administration of CL was found to effectively increase the abundance and diversity of the small intestinal flora, modify its structural composition, reduce the relative abundance of PTZ-elevated microflora, and enhance the abundance of Streptococcus, Staphylococcus, and Gemella Berger. Conclusion CL exhibits promising potential, reliability, and unique value in managing epilepsy through the regulation of intestinal flora.
Complete genome sequence of Schaalia odontolytica isolated from subgingival biofilm
Authors: Jang Eun-Young , Chun Jeewan , Kwack Kyu Hwan , Moon Ji-Hoi , Lee Jae-Hyung 
Published: February 2024

Abstract
Objective
Recent advancements in genome-based taxonomic classification propose the reclassification of certain Actinomyces species into new genera, including Schaalia. Schaalia odontolytica, the type species within this genus, is frequently found in the human oral cavity and has been associated with actinomycotic lesions. Currently, only two complete genomes of S. odontolytica strains have been reported. Recognizing the limited research on subspecies-level variation of S. odontolytica, we conducted genome sequencing of strain KHUD_008, isolated from a Korean periodontitis patient’s subgingival biofilm. Additionally, we performed a comparative genome analysis using previously sequenced genomes of strain XH001 and strain FDAARGOS_732, both derived from the human oral cavity.

Data description
Pacific Biosciences Sequel II sequencing generated 15,904 and 76,557 raw sequencing sub-reads, which were integrated to assemble the de novo genome using the Microbial Genome Analysis pipeline in the Single-Molecule Real-Time Analysis. The genome assembly completeness, assessed by Benchmarking Universal Single-Copy Orthologs, reached 99.2%. The genome is 2,389,595 bp with a GC content of 66.37%, and contains 2,002 protein-coding genes, 9 rRNAs, and 48 tRNA. Comparative analysis with two previously sequenced strains revealed many strain-specific genes in KHUD_008, primarily related to envelope biogenesis and replication/recombination/repair processes.
Characterization and transformation of TtMYB1 transcription factor from Tritipyrum to improve salt tolerance in wheat
Authors: Mu Yuanhang , Shi Luxi , Tian Huan , Tian Huaizhi , Zhang Jv , Zhao Fusheng , Zhang Qingqin , Zhang Suqin , Geng Guangdong 
Published: February 2024

Abstract
Background
Common wheat (Triticum aestivum L.) is a worldwide cereal crop, which is an integral part of the diets of many countries. In addition, the MYB gene of wheat plays a role in the response to salt stress.

Results
“Y1805” is a Tritipyrum variety that is relatively tolerant to salt. We used transcriptome analysis to show that the “Y1805” MYB gene was both highly expressed and sensitive to salt stress. Compared with control roots, the level of MYB expression during salt stress was higher, which rapidly decreased to control levels during the recovery process. MYB gene relative expression showed the highest levels in “Y1805” roots during salt stress, with the stems and then leaves being the next highest stressed tissues. The novel MYB gene (TtMYB1) was successfully cloned from “Y1805”. It showed a coding sequence length of 783 bp with 95.79% homology with Tel2E01G633100 from Thinopyrum elongatum. TtMYB1 and MYB from Th. elongatum were clustered in the same branch using phylogenetic analysis, which indicated high similarities. The TtMYB1 gene is located in the nucleus. The coleoptile method was employed when a TtMYB1 overexpression vector was used during transformation into “1718” (common wheat). Under high salt stress, TtMYB1 leaves of overexpression lines had decreased wilting, when compared with wild-type (WT) plants. During normal conditions, salt stress, and recovery, the lengths of the roots and the heights of seedlings from the overexpression lines were found to be significantly greater than roots and seedlings of WT plants. In addition, during high salt stress, the overexpression lines showed that proline and soluble sugar levels were higher than that of WT plants, but with lower malondialdehyde levels. Forty-three proteins that interacted with TtMYB1 were identified using the yeast two-hybrid assay. Protein-protein interaction analyses indicated that most were SANT domain-containing and Wd repeat region domain-containing proteins. Among these proteins, ribosomal proteins were the main node. Abiotic stress-related terms (such as “carbonate dehydratase activity”, “protein targeting peroxisomes”, and “glutathione peroxidase activity”) were enriched in GO analysis. In KEGG analysis, “carbohydrate metabolism”, “environmental information processing”, “genetic information processing”, “signaling and cell precursors”, and “energy metabolism” pathways were enriched.

Conclusion
The TtMYB1 gene might enhance salt tolerance by increasing proline and soluble sugar content and antioxidase activity in transgenic wheat. It therefore has the potential to enhance high salt tolerance in plants.
Social relationships and subjective wellbeing of the older adults in India: the moderating role of gender
Authors: Saha Shrestha 
Published: February 2024

Abstract
Objective
Social relationships have been extensively researched as having compelling health benefits. Nevertheless, these issues are understudied in low-income countries like India. This study investigates whether intergenerational solidarity or the social relationship developed in the household context and social participation or the social relationships developed in the community context are the critical determinants of subjective wellbeing (SWB) of older adults in India. In addition, this study also investigates whether gender moderates the association between social relationships and subjective wellbeing (SWB).

Methods
This study utilises a latent variable approach to investigate the association between social relationships and SWB among older adults (aged 60 or older, N = 9,852) based on a cross-sectional analysis of data from Building a Knowledge Base on Population Ageing in India (BKPAI) (2011). I tested the moderating effect of gender by employing a multigroup analysis in structural equation modelling that incorporated social relationships and other predictors of SWB.

Results
The study suggests that social participation has a significant protective impact on elderlies’ SWB, and gender doesn’t moderate the relation. Intergenerational solidarity has a significant health protective impact on older adults’ SWB; there is a significant moderating impact of gender. Specifically, functional and consensual solidarity have significant moderating effects of gender. Affectional solidarity, although a significant predictor of older adults’ SWB, and gender don’t moderate the relation.

Conclusion
The study suggests taking into account a variety of social relationships as well as gender roles when attempting to understand SWB at a later age. The results are discussed in terms of how gender roles impact social relationships and how men and women evaluate SWB differently based on those relationships.
ADHD medicine consumption in Europe after COVID-19: catch-up or trend change?
Authors: Gimbach Sophie , Vogel Daniel , Fried Roland , Faraone Stephen V. , Banaschewski Tobias , Buitelaar Jan , Döpfner Manfred , Ammer Richard 
Published: February 2024

Abstract
Background
Although the COVID-19 pandemic and its implications have been associated with mental health services utilization and medication consumption, there is no longitudinal study on the long-term impact on ADHD medication use trends.

Methods
This study examines the European ADHD medication consumption in 2020 to 2022 compared to the predicted consumption assuming the persistence of pre-pandemic trends. Predictions are calculated using Seasonal Autoregressive Integrated Moving Average (SARIMA) models.

Results
While European ADHD medication sales recorded a drop in 2020, they returned to the predicted level in 2021, even slightly exceeding it. In 2022, we found a clear exceedance of the predicted level by 16.4% on average at country level. Furthermore, the increase in consumption growth in the post-pandemic period (2021–2022) compared to the pre-pandemic period (2014–2019) was significant in 26 of the 28 European countries under consideration.

Conclusion
There is strong evidence of a trend change in the ADHD medicine consumption growth throughout Europe after the COVID-19 pandemic.
Intimate partner violence influences modern family planning use among married women in Tanzania: cross-sectional study
Authors: Baritwa Mrimi S. , Joho Angelina A. 
Published: February 2024

Abstract
Background
Married women who experience intimate partner violence (IPV) are less likely to negotiate with their partners on modern family planning (FP) use. This study aimed to determine the influence of intimate partner violence and sociodemographics on modern family planning use.

Methods
A community-based cross-sectional study was conducted in the Mara region, Tanzania from April to July 2020. A total of 366 married women were interviewed. Data were collected using a structured interviewer-administered questionnaire. Analysis was done using SPSS version 25, and a binary logistic regression model was used to determine the predictors of modern FP use. The significance level was set at a p-value less than 0.05.

Results
The overall prevalence of IPV was 73% with 54.1% physical, 36.3% psychological, and 25.4%, sexual violence. The prevalence of modern FP use was 62%, and the most (49.1%) common method practiced by married women was injection (Depo Provera). Physical violence (AOR = 0.32, p = 0.0056), and psychological violence (AOR = 0.22, p = 0.0022) had significantly reduced odds of modern FP use. Religion (AOR = 4.6, p = 0.0085), and availability of preferred modern FP methods (AOR = 9.27, p < 0.0001) had significantly increased odds of modern FP use.

Conclusion
In this study, there is a positive association between the use of modern FP methods and IPV. To prevent IPV and its negative health consequences, it is crucial to involve community leaders and primary healthcare workers. They can help in identifying the best strategies to prevent IPV and promote the use of modern FP methods. It is equally important to involve male partners in reproductive health decisions, including the use of modern FP methods. This approach will help reduce reproductive coercion.
Prostate cancer presentation and management in the Middle East
Authors: Sayan Mutlay , Langoe Astrid , Aynaci Ozlem , Eren Ayfer Ay , Eren Mehmet Fuat , Kazaz Ilke Onur , Ibrahim Zainab , Al-Akelie Omar Tama , Al-Mansouri Loma , Abu-Hijlih Ramiz , Moningi Shalini , Abou Chawareb Elia , El Hajj Albert , Orio Peter F. , Mula-Hussain Layth 
Published: February 2024

Abstract
Background
Although prostate cancer is a prevalent malignancy worldwide, its clinical presentation and management in the Middle East are not well-documented. This study aims to provide insights into the initial clinical presentation and management of prostate cancer in this region.

Methods
A retrospective review was conducted on seven institutional databases from six Middle Eastern countries, including Türkiye, Lebanon, Iraq, Syria, Bahrain, and Jordan, to identify patients diagnosed with prostate cancer in 2021. Descriptive analysis was performed on the collected data to provide an overview of the demographic, clinical, and treatment variables.

Results
A total of 1,136 patients were identified with a median age of 70 (range, 50–84). Most patients (78%) received their prostate cancer diagnosis after presenting with symptoms, as opposed to routine PSA screening. At the time of diagnosis, 35% of men had clinical T3 or T4 disease, 54% with Stage IV disease and 50% with Gleason score ≥ 8. Regarding treatment, 20% of non-metastatic and 22% of metastatic patients received no treatment.

Conclusion
Most men in this study sought prostate cancer evaluation due to symptoms and were subsequently diagnosed with advanced-stage disease, providing a foundation for future research aimed at understanding the underlying factors behind the observed trends and enabling informed interventions.