bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway
Authors: Tian Jiao , He Yiming , Zhang Zihui , Zhu Yuxin , Ren Haixia , Zhang Liang , Li Lei , Li Wei , Zhang Weidong , Xiao Ting , Zhou Honggang , Li Xiaoping 
Published: January 2025

ABSTRACTBackgroundOur study aimed to explore the specific functions and potential mechanisms of miR‐224‐5p in non‐small cell lung cancer (NSCLC).MethodsWe first analyzed the expression of miR‐224‐5p in NSCLC patients and cell lines through the GEO database and qRT‐PCR analysis. Then, we used MTT assays, wound healing assays, Transwell assays, and western blotting to evaluate the effects of miR‐224‐5p on NSCLC cell proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT). Furthermore, we used a xenograft tumor model to evaluate the effect of miR‐224‐5p on NSCLC tumor growth. Potential binding targets of miR‐224‐5p were further identified through the target prediction databases, and the relationships between miR‐224‐5p, its targets, and downstream signaling pathways were further verified using luciferase reporter gene assays and western blotting.ResultsThe GEO database and qRT‐PCR analysis indicated that miR‐224‐5p was significantly downregulated in NSCLC patients and cell lines. Functional assays indicated that inhibiting miR‐224‐5p could enhance the proliferation, migration, invasion, and EMT of NSCLC cells, as well as accelerate tumor growth. In contrast, overexpression of miR‐224‐5p inhibited these processes. We identified IL6ST (interleukin 6 signal transducer) as a binding target of miR‐224‐5p. We observed that miR‐224‐5p could bind to and inhibit IL6ST expression and JAK2/STAT3 signaling pathway, and the inhibition of NSCLC tumor growth and JAK2/STAT3 pathway by miR‐224‐5p could be reversed by IL6ST overexpression.ConclusionOur study demonstrated that miR‐224‐5p inhibited NSCLC by targeting IL6ST, thereby downregulating the JAK2/STAT3 signaling pathway.
Impact of EML4‐ALK Variants and TP53 Status on the Efficacy of ALK Inhibitors in Patients With Non‐small Cell Lung Cancer
Authors: Zou Zihua , Wu Lige , Hao Xuezhi , Li Yan , Liang Li , Gu Yangchun , Ying Jianming , Li Junling , Xing Puyuan 
Published: January 2025

ABSTRACTBackgroundThe clinical implications of different EML4‐ALK fusion variants remain poorly elucidated in the era of second‐generation ALK inhibitors.MethodsThis was a retrospective cohort study, wherein patients diagnosed with locally advanced or metastatic non‐small cell lung cancer harboring EML4‐ALK fusion were stratified into two cohorts based on their first‐line treatment: Cohort 1 received alectinib, while Cohort 2 received crizotinib. Statistical analysis was employed to investigate the impact of different EML4‐ALK variants and TP53 status on the efficacy of first‐line ALK‐TKIs.ResultsFinally, 49 patients were enrolled in cohort 1 and 53 patients in cohort 2. In cohort 1, patients with long EML4‐ALK fusion variants exhibited prolonged PFS (NR vs. 34.0 m, p = 0.004, HR = 0.30, 95% CI: 0.12–0.74) and an elevated 5‐year OS rate (93.3% vs. 68.4%, p = 0.020, HR = 0.12, 95% CI: 0.02–0.62) compared to those with short variants. The median PFS was not reached in TP53‐wt group and 47.0 m in TP53‐mut group (p = 0.087, HR = 0.44, 95% CI: 0.17–1.17). The TP53‐wt group exhibited a superior 5‐year OS rate (100% vs. 77.8%, p = 0.030) compared to TP53‐mut group. In cohort 2, the median PFS was 14.0 m in long variant group and 12.9 m in short variant group (p = 0.094, HR = 0.65, 95% CI: 0.37–1.13); the median OS was not reached in long variant group and 69.2 m in short variant group (p = 0.254, HR:0.62, 95% CI: 0.27–1.42). However, the efficacy of first‐line crizotinib did not appear to be influenced by the TP53 status.ConclusionsEML4‐ALK short variants and TP53 mutations are both adverse factors for first‐line alectinib efficacy, but they have little effect on first‐line crizotinib.
The reciprocal within‐person relationship between job insecurity and life satisfaction: Testing loss and gain spirals with two large‐scale longitudinal studies
Authors: Jiang Lixin , Xu Xiaohong , Zubielevitch Elena , Sibley Chris G. 
Published: January 2025

AbstractDeriving from the loss and gain spirals of conservation of resources (COR) theory, this research attempts to understand the within‐person, reciprocal relationship between job insecurity and life satisfaction. Using three independent samples from seven‐wave, five‐wave, and 21‐wave annually collected data from New Zealand and Australia, and random intercept cross‐lagged panel models, we find that (1) job insecurity has a negative concurrent relationship with life satisfaction at the between‐person level; (2) job insecurity at an earlier time point has a negative relationship with life satisfaction and a positive relationship with job insecurity at a later time point, indicating that individuals facing an initial threat of resource losses are more prone to further resource depletion, supporting “loss spirals” in COR theory; and (3) life satisfaction at an earlier time point has a positive relationship with life satisfaction at a later time point, supporting “gain spiral” in COR theory. However, life satisfaction is not longitudinally related to job insecurity at a later time point, failing to support the reverse causality. By rigorously investigating the reciprocal within‐person relationship between job insecurity and life satisfaction with two large‐scale panel data sets, these findings strongly support the loss and gain spirals from COR theory.
Gossypin Ameliorates Collagen‐Induced Arthritic Rats by Inhibiting Angiogenesis, Inflammation and Oxidative Stress
Authors: He Caiyou , Kumar Ankit 
Published: January 2025

ABSTRACTBackgroundRheumatoid arthritis (RA) is a long‐term inflammatory autoimmune disease that damages cartilage and synovial membranes while also affecting bones and joints. The aim of the current study was to investigate the antiarthritic effect of gossypin against collagen‐induced arthritis (CIA) in rats.MethodsIntraperitoneal administration of Type II collagen (2 mg/mL) was used to induce arthritis in the rats, followed by oral administration of gossypin (5, 10 and 15 mg/kg) for 28 days. Various parameters were assessed, including body weight, paw swelling, arthritis score, organ weights, RF, haematological parameters, inflammatory markers, MMP levels, antioxidants, cytokines and nonhepatic and hepatic liver parameters. Additionally, the expression of various mRNAs was analysed.ResultsGossypin significantly (p < 0.001) altered the body weight, paw swelling, arthritis score and organ weights along with RF level. Gossypin treatment significantly (p < 0.001) altered the level of antioxidant and hepatic parameters; inflammatory cytokines; and inflammatory parameters such as PGE2, COX‐2, NF‐κB and VEGF, respectively. Gossypin also enhanced the level of HO‐1 and Nrf2 and suppressed the level of MMP parameters such as MMP‐2, MMP‐3 and MMP‐9. Gossypin also modified the mRNA expression of MMP‐3, MMP‐9, TRAP, RANK, Ctsk and RANKL, respectively.ConclusionGossypin demonstrated antiarthritic effects against CIA in rats.
Effects of the sequential use of L1 and bilingual subtitles on incidental English vocabulary learning: A cognitive load perspective
Authors: Yuan Xin , Tang Xuan 
Published: January 2025

AbstractAimFrom the perspective of cognitive load theory, the present study examined the relative effectiveness of the sequential use of L1 and bilingual subtitles on incidental English vocabulary learning.MethodsA total of 162 upper‐intermediate Chinese learners of English as a foreign language watched an English clip in one of 4 subtitling conditions: L1‐bilingual, bilingual‐bilingual, L2‐L2, and no subtitles.ResultsResults suggested a statistically significant advantage for the L1‐bilingual condition over other conditions for word form and meaning recall. The sequential use of L1 and bilingual subtitles also elicited the lowest cognitive load. This study suggests that the viewing sequence that starts with L1 subtitles might increase the efficiency of viewing‐based incidental vocabulary learning and have the potential to reduce cognitive load.
Sand Play and 0‐ to 8‐Year‐Old Children's Physical, Cognitive and Socioemotional Outcomes: A Mixed‐Methods Systematic Review
Authors: Iivonen Susanna , Kettukangas Titta , Soini Anne , Viholainen Helena 
Published: January 2025

ABSTRACTBackgroundSand play (SP) is a global phenomenon that may be linked to children's health outcomes, but limited evidence exists. The purpose was to synthesize evidence on the association between SP and 0‐ to 8‐year‐old children's physical, cognitive and socioemotional outcomes.MethodsCINAHL, Academic Search Premier, PsycINFO, SPORTDiscus with Full Text, ERIC, Medline, Scopus and Google Scholar were searched. Studies were eligible if 0‐ to 8‐year‐old children's outcomes were reported while attending SP. Synthesis was performed using effect direction (quantitative), thematic (qualitative), narrative and results‐based convergent synthesis incorporating the risk of bias and confidence in the evidence assessments. Studies were grouped by SP exposure context as education, leisure and rehabilitation.ResultsThe effect direction (Nstudies = 17; Nchildren = 677), thematic (Nstudies = 8; Nchildren = 131) and narrative (Nstudies = 8; Nchildren = 11 784) syntheses included 28 studies published in 1993–2023 (Ntotal = 12 646; Mchildage = 3–6 years; Mgirls 41%; Methnicity Asian 3%, African American/Black/Hispanic 8%, European American/White/Non‐Hispanic 56%, Multi‐ethnic 36%). Based on very low to moderate confidence in the evidence, findings indicated inconsistent associations between educational, rehabilitational and leisure SP and children's physical, cognitive and socioemotional outcomes. There was inconsistent evidence on the occurrence of SP by children's gender and age.ConclusionSP may enhance some childhood outcomes. However, high‐quality controlled designs describing the exposure to SP are needed to inform the effectiveness of SP on children's health and development.
Hereditary Vitreoretinopathies: Molecular Diagnosis, Clinical Presentation and Management
Authors: Ghoraba Hashem H. , Sears Jonathan , Traboulsi Elias I. 
Published: January 2025

ABSTRACTHereditary vitreoretinopathies (HVRs), also known as hereditary vitreoretinal degenerations comprise a heterogeneous group of inherited disorders of the retina and vitreous, collectively and variably characterised by vitreal abnormalities, such as fibrillary condensations, liquefaction or membranes, as well as peripheral retinal abnormalities, vascular changes in some, an increased risk of retinal detachment and early‐onset cataract formation. The pathology often involves the vitreoretinal interface in some, while the major underlying abnormality is vascular in others. Recent advances in molecular diagnosis and identification of the responsible genes and have improved our understanding of the pathogenesis, risks and management of the HVRs. Clinically, HVRs can be classified according to the presence or absence of skeletal or other systemic abnormalities, retinal dysfunction or retinal vascular abnormalities [2]. There are some discrepancies in the literature regarding which diseases are included under the overarching term ‘hereditary vitreoretinopathies’. Conditions such as Stickler syndrome, Wagner syndrome and familial exudative vitreoretinopathy are generally included, while others such as autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV) and autosomal dominant vitreoretinochoroidapathy (ADVIRC) may not. In this review, we will discuss some historical aspects, the molecular pathogenesis, clinical features and management of diseases and syndromes commonly considered as HVRs.
Microgap Formation in Conical Implant‐Abutment Connections Under Oblique Loading: Influence of Cone Angle Mismatch Through Finite Element Analysis
Authors: Gantier‐Takano Marlene Kasumi , Xing Yiyun , Ye Ning , Aparicio Conrado , Navarro Cuéllar Carlos , Meira Josete Barbosa Cruz , Fok Alex Siu Lun 
Published: January 2025

ABSTRACTObjectivesThis study evaluated different designs of the conical implant‐abutment connection (IAC) and their resistance to microgap formation under oblique loads as specified by the ISO standard for testing dental implants. Also evaluated was the effect of deviations from the ISO specifications on the outcomes.MethodsFinite element analysis was conducted to compare the microgap formation and stress distribution among three conical IAC designs (A, B, and C) in two loading configurations: one compliant with ISO 14801 and one with a modified load adaptor (non‐ISO). The different IAC designs varied in the taper, diameter, and cone height. The cone angle mismatch (Cam) between the implant and abutment was considered. A torque of 20 Ncm and oblique loads (up to 400 N) were simulated.ResultsThe stresses produced by the screw‐tightening torque varied among the different IAC designs. The contact height was approximately 0.3 mm for Designs A and B, and less than 0.03 mm for Design C. Under oblique loads, Design A maintained IAC sealing without gap formation up to 400 N. With the ISO adaptor, gaps appeared in Design B at 300 N and in Design C at 90 N. The non‐ISO adaptor resulted in gap formation at 160 N for Design B and at 50 N for Design C.ConclusionsThe IAC design and cone angle mismatch significantly influenced microgap formation, with some designs showing zero gaps even when the oblique load reached 400 N. The non‐ISO adaptor increased gap formation in IACs B and C.
Molecular Symphony of Mitophagy: Ubiquitin‐Specific Protease‐30 as a Maestro for Precision Management of Neurodegenerative Diseases
Authors: Siwach Ankit , Patel Harit , Khairnar Amit , Parekh Pathik 
Published: January 2025

ABSTRACTIntroductionMitochondrial dysfunction stands as a pivotal feature in neurodegenerative disorders, spurring the quest for targeted therapeutic interventions. This review examines Ubiquitin‐Specific Protease 30 (USP30) as a master regulator of mitophagy with therapeutic promise in Alzheimer's disease (AD) and Parkinson's disease (PD). USP30's orchestration of mitophagy pathways, encompassing PINK1‐dependent and PINK1‐independent mechanisms, forms the crux of this exploration.MethodA systematic literature search was conducted in PubMed, Scopus, and Web of Science, selecting studies that investigated USP's function, inhibitor design, or therapeutic efficacy in AD and PD. Inclusion criteria encompassed mechanistic and preclinical/clinical data, while irrelevant or duplicate references were excluded. Extracted findings were synthesized narratively.ResultsUSP30 modulates interactions with translocase of outer mitochondrial membrane (TOM) 20, mitochondrial E3 ubiquitin protein ligase 1 (MUL1), and Parkin, thus harmonizing mitochondrial quality control. Emerging novel USP30 inhibitors, racemic phenylalanine derivatives, N‐cyano pyrrolidine, and notably, benzosulphonamide class compounds, restore mitophagy, and reduce neurodegenerative phenotypes across diverse models with minimal off‐target effects. Modulation of other USPs also influences neurodegenerative disease pathways, offering additional therapeutic avenues.ConclusionsIn highlighting the nuanced regulation of mitophagy by USP30, this work heralds a shift toward more precise and effective treatments, paving the way for a new era in the clinical management of neurodegenerative disorders.
How Do Trematodes Induce Cancer? A Possible Evolutionary Adaptation of an Oncogenic Agent Transmitted by Flukes
Authors: Apari Péter , Földvári Gábor 
Published: January 2025

ABSTRACTThere is strong epidemiological evidence that development of various cancer types is linked to infection with flukes (Platyhelminthes: Trematoda) in Africa, Asia and the Middle East. The exact nature of the mechanism by which cancer is induced by these parasites is unknown. Here, we provide a new hypothesis suggesting that flukes are not the primary cause of cancer but act as vectors of cancer‐inducing microbial pathogens. These pathogens adaptively induce tumours to attract and help flukes to feed on blood from the tumour. Pathogen take‐up by fluke vectors also takes place in the tumour; therefore, tumour formation in this case is the result of a mutualistic and adaptive relationship between the microbe and the helminth parasite. The suggested mechanism for cancer induction provided here may help us gain deeper understanding about cancer in general and its relationship with microbes and parasites. By further elaborating the unique nexus between flukes, carcinogenic microbes and cancer, in the future it will also help us to broaden our oncological perspective to reduce human death and suffering from this serious disease group.
Predictive value of the Hemoglobin, Albumin, Lymphocyte and Platelet score for mortality in geriatric patients presenting to the emergency department
Authors: Tortum Fatma , Tekin Erdal , Gur Ali 
Published: January 2025

AimThe Hemoglobin, Albumin, Lymphocyte and Platelet (HALP) score, calculated as hemoglobin × albumin × lymphocytes / platelets, serves as a novel biomarker that can provide insights into a patient's nutritional status, anemia status and inflammatory processes. This study aimed to investigate the predictive value of the HALP score for mortality among geriatric patients presenting to the emergency department.MethodsThis retrospective study was carried out at the emergency department of a tertiary hospital. Patients aged ≥65 years who presented to the emergency department between 1 January 2018 and 1 January 2024 were included in the study. A total of 62 262 patients who visited our emergency department were enrolled. Patient data, including hemoglobin, albumin, lymphocyte and platelet values; age; sex, the reason for hospital presentation; and outcome (mortality or discharge) were obtained from electronic medical records. HALP scores were calculated for the patients, and statistical analyses were carried out.ResultsOf the patients, 32 410 were men, and the mean age was 73 years. Within this cohort, in‐hospital mortality occurred in 3093 of the patients. The HALP score was significantly lower in patients who died compared with those who were discharged (P < 0.001).ConclusionDue to its cost‐effectiveness and ease of calculation, the HALP score appears to be more feasible in predicting mortality in the emergency department compared with other scoring systems. Geriatr Gerontol Int 2025; ••: ••–••.
Factors Associated With Low Childbirth Self‐Efficacy for Vaginal Birth in High‐Risk Pregnant Women
Authors: Sarıboğa Yılmaz , Gürkan Zeynep , Sarıkaya Remzi 
Published: January 2025

ABSTRACTBackgroundLow childbirth self‐efficacy is a significant indicator of vaginal birth and is closely related to adverse perinatal outcomes.ObjectivesThis study aimed to evaluate factors associated with low childbirth self‐efficacy for vaginal birth in high‐risk pregnant women.MethodsThis descriptive study was conducted between July and December 2022 in Van, Turkey, with a total of 200 participants. Data collection instruments included a Socio‐demographic Questionnaire and the Self‐Efficacy Regarding Vaginal Birth (SEVB) scale. Independent t‐test, chi‐square test and multivariable binary logistic regression were employed for data analysis.ResultsThe mean age of the participants was 27.74 ± 5.12 years, and the mean gestational age was 33.22 ± 4.28 weeks. Unplanned pregnancy status (p = 0.002), a history of caesarean section (p = 0.013), multiparity (p = 0.001), age (p < 0.01) and gestational age (p = 0.001) were associated with low childbirth self‐efficacy for vaginal birth. In multivariate analysis, only age (B: 1111; 95% CI: 1038–1189; p = 0.002) and gestational age (B: 1120; 95% CI: 1034–1214; p = 0.004; and p = 0.005) were independently associated with low childbirth self‐efficacy.ConclusionsLow childbirth self‐efficacy for vaginal birth was associated with higher maternal age and gestational ages. Identifying high‐risk pregnant women with low childbirth self‐efficacy will improve the care process for women and their newborns.
Assessing the Impact of Non‐Pharmaceutical Interventions During the COVID‐19 Pandemic on RSV Seasonality in Europe
Authors: Heemskerk Susanne , Baliatsas Christos , Stelma Foekje , Nair Harish , Paget John , Spreeuwenberg Peter 
Published: January 2025

ABSTRACTBackgroundDuring the COVID‐19 pandemic, atypical respiratory syncytial virus (RSV) circulation patterns emerged, with the occurrence of RSV activity outside the typical winter season. This study investigates the impact of COVID‐19 and associated non‐pharmaceutical interventions (NPIs) on RSV seasonality.MethodsThe onset, offset and peak of RSV epidemics from 2018 to 2022 across 12 European countries were determined using the 3% positivity threshold method. A multilevel longitudinal logit regression model for proportions assessed the associations between five NPIs (school closures, mask use, workplace measures, public gathering restrictions and closure of public spaces) and RSV, utilising RSV surveillance data, two NPI databases (ECDC‐JRC and Oxford) and COVID‐19 surveillance data.ResultsBefore 2020, consistent RSV seasonality patterns were observed, but the seasonal increase of RSV‐positive cases in winter remained absent during the COVID‐19 pandemic (2020–2022). Analysis revealed inconsistent associations between individual NPIs and RSV. The associations differed depending on the data source used (ECDC‐JRC or Oxford), not only in magnitude but also in the direction of the coefficients. Public gathering restrictions and closure of public spaces exhibited significant negative associations with RSV incidence. However, this was only observed when using surveillance data for the entire epidemiological year and not when only examining weeks with increased RSV activity.ConclusionsThis study highlights the need for standardised international data collection and procedures for infectious disease modelling, as varying NPI implementations, NPI registration and RSV surveillance across countries complicate the understanding of RSV dynamics during the pandemic. Caution is recommended when interpreting the effects of NPIs on RSV circulation.