bluuebunny/crossref_metadata_2025_split huggingface dataset:
The press-assisted fusion scheme significantly mitigates the quantity of HVJ-E required in mitochondrial replacement techniques
Authors: Ma Meng , Jiang Shutian , Jin Wei , Li Wenzhi , Chen Chen , Lin Kaibo , Liao Xiaoyu , Li Danjun , Kuang Yanping , Lyu Qifeng 
Published: January 2024

In brief
The impact of HVJ-E employed in mitochondrial replacement techniques (MRTs) on embryonic development remains uncertain. This study has exhibited the influence of HVJ-E utilized in MRTs on embryonic development and has devised a novel HVJ-E-induced fusion approach to curtail the amount of HVJ-E employed in MRTs.

Abstract
Mitochondrial replacement techniques (MRTs) provide a viable option for women carrying pathogenic mitochondrial DNA (mtDNA) variants to conceive disease-free offspring with a genetic connection. In comparison to electrofusion, HVJ-E-induced fusion has been identified as the most promising approach for clinical translation of MRTs due to its absence of electrical interference. However, despite confirmation of the absence of RNA activity in HVJ-E, a reduction in blastocyst quality has been observed in various MRTs studies utilizing the HVJ-E-induced fusion scheme. Recent investigations have revealed a dose-dependent elevation of reactive oxygen species (ROS) levels in various cancer cells incubated with HVJ-E. However, the impact of HVJ-E as a sole determinant on embryonic development in MRTs remains unverified. This investigation establishes that the augmented concentration of HVJ-E utilized in the conventional HVJ-E fusion protocol is an autonomous variable that influences embryonic development in MRTs. This effect may be attributed to amplified DNA damage resulting from heightened levels of ROS in reconstructed embryos. To mitigate the presence of HVJ-E in reconstructed zygotes while maintaining optimal fusion efficiency in MRTs, a novel HVJ-E-induced fusion approach was devised, namely, press-assisted fusion. This technique offers potential advantages in reducing detrimental factors that impede embryo development in MRTs.
USE OF VIRTUAL REALITY TECHNOLOGIES TO RESTORE COGNITIVE FUNCTIONS AND QUALITY OF LIFE: AN APPLICATION FOR CARDIAC PATIENTS WITH BRAIN ISCHEMIA
Authors: Razumnikova Olga M. , Trubnikova Olga A. 
Published: January 2024

HighlightsWe present an overview of publications on neurological and cardiac rehabilitation programs using virtual reality technologies. Training that involves multitasking and combining cognitive and physical tasks with interactive scenarios is most effective in terms of the improvement of the quality of life of patients with coronary artery disease. Ecotherapy in virtual environment is a promising way to reduce anxiety and stress. The review presents the analysis of publications focused on neurological and cardiac rehabilitation programs using virtual reality technologies (VRT), involving the integration of multisensory and multidomain tasks, as well as a meta-analysis of such studies. It is shown that VRTs make it possible to simulate interactive scenarios for simulating vigorous activity with given visual, tactile and kinesthetic sensations and with the appropriate correction in accordance with the needs of various users. It is noted that when developing programs based on VRT, it is necessary to take into account the degree of immersion of users in the virtual environment and symptoms of virtual reality sickness.The analysis of meta-reviews of published articles indicates the need to continue research in this field due to the wide variety of rehabilitation programs, used indicators of cognitive functions and health status in heterogeneous populations of healthy individuals and cardiac patients involved in training.The results obtained allow us to conclude that neurological and cardiac rehabilitation is the most effective when it involves training with multitasking and combined cognitive and physical tasks in VR. Ecotherapy in virtual environment is a promising way to reduce anxiety and stress.
ROLE OF HYPOTHERMIA IN CEREBRAL PROTECTION IN SURGICAL CORRECTION OF CONGENITAL HEART DEFECTS
Authors: Ivkin Artem A. , Grigoriev Evgeny V. 
Published: January 2024

HighlightsThis article is devoted to a review of studies on the mechanisms of cerebral protection and the effects of hypothermia on the patient. AbstractA large number of surgical corrections of congenital heart defects are performed around the world annually, most of them using cardiopulmonary bypass. This kind of surgery can be a pathological factor for the brain. In some patients, the kind of surgery involves the use of hypothermia. Supposedly, hypothermia should have a neuroprotective effect, however, as recent studies show, this is not always the case. This review is devoted to the mechanisms of the effect of hypothermia on the patient’s body, including not only a decrease in brain metabolism, but also a number of others. Moreover, a review of current works on the use of hypothermia in clinical practice in surgeries that do not require circulatory arrest is presented, along with an analysis of the intra- and postoperative period.
Young Entrepreneur Transition to Youth NEET During Covid-19 Pandemic: Evidence from Indonesia
Authors: Putranto FX Gugus Febri , Pratomo Devanto Shasta , Pudjihardjo M 
Published: January 2024

The global crisis caused by the pandemic also has an impact on the labor market in Indonesia. Young and women workers are strongly affected by the crisis and economic uncertainty due to the pandemic. One of the perceived impacts is the transition of young workers from entrepreneurs to NEET. This study aims to analyze the determinants of the transition of young entrepreneurs to NEET during the pandemic in Indonesia, both in general and gender-specific models. Data from the National Labor Force Survey (SAKERNAS) with individual panel observation in the period of August 2019 and August 2020 were analyzed using multinomial logistic regression. Important issues related to the determinants of the transition from an entrepreneur to NEET in Indonesia are demographic characteristics, COVID-19 risk status in the area of residence, and employment characteristics. Meanwhile, in the gender-specific model, there are differences in the determinants between male and female models. Prevention of the transition to NEET can be done by improving skills and education for young entrepreneurs, one of which is by expanding the scope of kartu prakerja. Specifically, on gender-based policy, women can be directed to the creative economy with flexible working hours
Study of the effect of bacteria of the genus <i>Pseudomonas</i> on the growth and toxin production of the fungus <i>Fusarium graminearum</i> <i>in vitro</i>
Authors: Tolkach V. F. , Volkov Yu. G. , Kakareka N. N. , Aliev M. R. , Shchelkanov M. Yu. 
Published: January 2024

Aim. To study the potential of bacteria strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to inhibit the growth of Fusarium ear blight pathogens through the example of the fungus F. graminearum and to reduce the accumulation of deoxynivalenol (DON) and zearalenone (ZEN) in vitro. Materials and Methods. Antifungal metabolites of Pseudomonas bacteria were analysed by thin layer chromatography and bioautography. An experiment on the effect of liquid culture and supernatant of bacteria on the growth and toxin production of the fungus F. graminearum in vitro was carried out on wheat grain. Analysis of grain for mycotoxins was carried out by HPLC‐HRMS. Results. The ability of strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to produce antifungal metabolites was found, while inhibiting the growth of the fungus F. graminearum 60318 in vitro. The content of DON decreases both under the action of the liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 60 % and 70 %, respectively, and the liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria by 75 % and 90 %, respectively. The accumulation of ZEN is also significantly suppressed under the influence of liquid cultures and supernatants of bacterial strains. The amount of ZEN decreases under the influence of liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 80 % and 95 %, respectively. Liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria inhibited the accumulation of ZEN by 60 % and 84 %, respectively. Conclusion. The results obtained through the study of the effect of liquid cultures and supernatants of P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 strains on the growth and toxin production of the fungus F. graminearum 60318 in vitro allow us to consider these strains as potential producers of effective biofungicides against toxin‐producing fungi. Further studies of these bacterial strains in vitro and in vivo on plants are needed.
Assessment of the incidence of leukemia virus (BLV) in cattle in the Republic of Dagestan using PCR diagnostics
Authors: Baboshko D. A. , Elfimov K. A. , Daudova M. G. , Koychuev Kh. G. , Gapizova Kh. F.‐K. , Gashnikova N. M. 
Published: January 2024

Aim. Assessment of the incidence of leukemia virus in cattle using PCR diagnostics in herds of the Republic of Dagestan and study of the molecular genetic characteristics of circulating viruses. Materials and Methods. 150 cattle blood samples were examined. PCR diagnostics of samples for the presence of bovine leukosis virus (BLV) were carried out using the RealBest‐Vet DNA BLV test system and a laboratory set of primers. Some of the samples were sequenced using the Sanger method and their phylogenetic analysis was performed. Results. Out of 150 samples, 24 samples were positive for the presence of BLV. In the Untsukulskiy district, no BLV DNA was detected in any of the 16 samples. In the Karabudakhkentskiy district out of 40 – in 2 (5 %), in Buynakskiy district – in 1 out of 30 (3.3 %) and in Babayurtovskiy district–in 21 out of 60 samples BLV was detected (35 %). For 13 BLV‐positive samples, fragments of the env gene measuring 1000 bp were obtained and deciphered. According to phylogenetic analysis, 7 samples of BLV belong to the 7th, and 6 – to the 4th genotype of BLV. The BLV genotype 4 isolated in the Babayurtovskiy district clusters with viruses from Kazakhstan, while viruses of genotype 4 from other farms cluster with Russian BLV. The studied samples of genotype 4 did not form common clusters. For the BLV genotype 7 isolated in farms of the Babayurtovskiy district, on the contrary, a combination of sequences into one cluster of genetically similar viruses was observed. Conclusion. Significant differences in the incidence of leukemia virus in livestock on farms in Dagestan were revealed. No patterns were found in the registration of cattle cases with a specific breed of cattle or with the age of the animal. It has been shown that viruses of both genotypes 7 and 4 circulate in the Republic. For BLV 4, it is assumed that there are different ways of its introduction into farms but no associated chains of virus spread have been found. For BLV genotype 7, transmission of BLV has been registered, which indicates the need to strengthen leukemia prevention measures on farms.
Factors and prerequisites for the formation of transboundary specially protected natural areas in the Russian‐Kazakhstan region
Authors: Chibilev A. A. , Svyatokha N. Yu. , Grudinin D. A. 
Published: January 2024

Aim. To conduct a geographical analysis of the network of specially protected natural areas in the Russian‐Kazakhstan transboundary region and identify promising areas of international cooperation in the field of protection and conservation of natural geosystems. Material and Methods. The study was built on the basis of statistical processing of state statistics of Russia and Kazakhstan and on geoinformation analysis of open data on population and materials obtained from official geoinformation portals containing information on specially protected natural areas of both countries. Results. The authors have established and mapped border protected natural areas of federal/republican significance in the Russian‐Kazakhstan region. By means of GIS technologies, "zones of influence" of key nature protection areas were determined by constructing centroids of protected areas and Thyssen/Varzar polygons. Population levels of the territories within the obtained zones was calculated. Promising transboundary specially protected territories have been identified. Mechanisms for drawing attention to the problems of nature protection are proposed. Conclusions. The results of the analysis of the parameters of the population of the territory near the most significant protected areas in the border zone of Russia and Kazakhstan are of practical importance in the integration of specially protected natural areas into the socio‐economic development of the regions using the experience and traditions of the local population and taking into account their interests.
Enteroviruses in the etiology of acute respiratory viral infections in children, Novosibirsk, Russia
Authors: Kurskaya O. G. , Saroyan T. A. , Nokhova A. R. , Dubovitskiy N. A. , Derko A. A. , Basova P. S. , Gutova T. A. , Solomatina M. V. , Murashkina T. A. , Sharshov K. A. 
Published: January 2024

Aim. To assess the contribution of human enteroviruses to the overall structure of acute respiratory viral infections in children in Novosibirsk during the epidemic season 2022–2023. Material and Methods. 1132 samples collected from children aged 0–15 years with symptoms of ARVI were analyzed by real–time PCR for the presence of genetic material of respiratory viruses, including enteroviruses. Results. 65.2 % of the examined children were positive for at least one virus, including enteroviruses. Respiratory syncytial virus was most often found in children during this period, which accounted for 17.2 % of the total number of samples studied. Influenza virus was detected in 14.2 % of the examined children. Enteroviruses were detected in 9.2 % of cases and rhinovirus was detected in 10.8 % of the examined children. Conclusions. Respiratory enteroviruses, along with rhinovirus, occupy a significant place in the etiology of acute respiratory viral infections in children. The study of the epidemiology of enteroviruses is necessary to understand the clinical manifestations and outcomes of enterovirus infection, and to assess the burden of diseases caused by enteroviruses, which is important for optimizing prevention and therapy strategies.
The content of chemical elements in <i>Trаpa nаtans</i> and <i>Nymphoides peltata</i> of the Volga Delta
Authors: Kuanova A. S. , Ershova T. S. , Zaitsev V. F. , Chaplygin V. A. , Litvinova N. V. 
Published: January 2024

Aim. To identify the specific features of the accumulation of heavy metals in Trаpa nаtans and Nymphoides peltata in the Volga delta. Materials and Methods. Plant sampling was carried out in the Astrakhan State Nature Biosphere Reserve. Determination of the concentration of chemical elements was carried out using atomic absorption spectrophotometry. Results. As a result of the research it was noted that the plant species studied had some similarities in the accumulation of chemical elements. In both types of plants iron was predominantly accumulated, manganese content being in second place in terms of metal concentration. Chromium and cadmium were found to be in the lowest concentrations. Nymphoides peltata is an iron accumulator compared to Trapa natans. The leaves of Trapa natans are manganese concentrators, while the stems are copper concentrators. Conclusions. Based on the specific features identified in the accumulation of chemical elements in plants, a proposal is put forward regarding the possibility of using the leaves and stems of Nymphoides peltata as a test object for monitoring iron and chromium concentrations in water and the leaves of Trapa natans for monitoring copper concentrations in water.
Key factors in the successful adaptation of the pest <i>Corythucha ciliata</i> Say in the northern subtropics of the Black Sea coast
Authors: Kashutina E. V. , Bugaeva L. N. , Khetagurova E. V. , Ignatieva T. N. 
Published: January 2024

Aim. To identify a complex of factors in the successful adaptation of the sycamore lace bug Corythucha ciliata Say for the development of methods for regulating its numbers of and effective biological control measures against this dangerous pest. Material and Methods. The research was carried out by studying scientific publications, analysing the dynamics of climate change in the Lazarevsky district of Sochi for 15 years and studying the reporting data of the Lazarevskaya Experimental Plant Protection Station, Branch of the Federal Research Center of Biological Plant Protection for the period 2008 to 2022. Results. 5 key factors of successful adaptation of Corythucha ciliata to new invasion regions have been identified: the factors of food and climatic adaptation, the factor of interaction with natural enemies, the factor of resistance to entomopathogenic organisms and the factor of adaptation to anthropogenic load. The main conditions and possible vectors of further invasion of sycamore lace in new regions have been determined. Conclusion. The development of effective methods of biological control of the invasive pest Corythucha ciliata Say should be based taking into account the totality of qualitative characteristics of the pest's living conditions. The sycamore lace bug Corythucha ciliata Say effectively builds new trophic connections, without prejudice to its population, adapts not only to new climatic conditions, but also to their changes. Natural entomophages and entomopathogens do not significantly affect the development of the Corythucha ciliata population. The pest has adapted to survival in conditions of high anthropogenic load and successfully uses it to invade new regions.
Productivity of vegetation and carbon stock in meadow steppe on fallow areas in the Bashkir Cis‐Urals (Southern Urals region), Russia
Authors: Baisheva E. Z. , Fedorov N. I. , Zhigunova S. N. , Shirokikh P. S. , Komissarov M. A. , Gabbasova I. M. , Muldashev A. A. , Bikbaev I. G. , Tuktamyshev I. R. , Shendel G. V. , Suleymanov R. R. , Garipov T. T. 
Published: January 2024

Aim. Data collection and analysis of the composition and productivity of vegetation and assessment of carbon sequestration by vegetation and soil in meadow steppe at a fallow site in the Bashkir Cis‐Urals, Russia. Material and Methods. The work is based on a survey of vegetation and soils of meadow steppe located in fallow areas, where post‐agrogenic restoration succession has been going on for more than 20 years. Results. The plant cover of the area studied is close to natural rich‐forb meadow steppes but differs in lower indicators of species richness and saturation, as well as the presence of weedy segetal species. The total plant biomass on the plot studied was estimated at 11.35 t/ha (including live aboveground biomass 2.98 t/ha, mortmass 3.11 t/ha, root weight 5.25 t/ha). The average stock of carbon in the plant biomass is 427.6 g/m2, and the total carbon stock in plant matter within the site is 36.88 tons. The soil cover of the area studied is Chernozem Calcic. The average levels of carbon stocks in short‐thickness soil at the 0–90 cm layer is 308 t/ha, while in medium‐thick soil it is 378 t/ha. Conclusions. A feature of the communities surveyed is a poor floristic composition, low productivity and low proportion of roots (47 % of the total plant biomass), which is caused by incomplete recovering of steppe vegetation and low thickness of the humus horizon. The soils of the site are close to virgin steppe lands in terms of carbon content and reserves.
Distribution of bats in the subtropical regions of the Black Sea coast and adjacent territories
Authors: Ivanitzky A. N. , Alekseev A. Yu. 
Published: January 2024

Aim. The Black Sea region is one of the centers of diversity of bats in the Palearctic. In this regard, the main aim of the work was determined ‐ to study the species composition of the bat fauna of the Black Sea region and the distribution of bat species there. The aim becomes especially relevant if we consider that significant changes have occurred in the composition of the region’s fauna in recent years and that the distribution of chiropterans in the Black Sea region as a whole has never been the object of special research. Discussion. The article contains point maps of the distribution of each of the 35 species of bats living in the subtropics of the Black Sea region and adjacent territories. In addition, the maps are provided with comments on the range of each species on the Black Sea coast. Conclusion. The data presented can be used both for fundamental research and for solving practical problems, primarily in the organization and implementation of environmental measures.
Retrospective Evaluation of Misdiagnosed Scabies Cases: Clinical and Epidemiological Features and Resemblance to Other Dermatological Conditions
Authors: GÜLBAŞARAN Fatmagül , ÖZBAĞCİVAN Özlem , ÖNER Samet , ÇAPKAN Onur 
Published: January 2024

Background and Aim: Diagnosing scabies, a skin condition caused by mites, can be challenging due to its resemblance to other skin problems. Avoiding misdiagnoses could result in a significant reduction in treatment delays and complications. Our objective was to investigate scabies cases that initially posed diagnostic challenges.
Methods: This retrospective study focused on scabies patients who were initially misdiagnosed despite routine clinical examinations and underwent histopathological examinations with different preliminary dermatological diagnoses. Inclusion criteria were in accordance with the 2020 International Alliance for the Control of Scabies Diagnosis Criteria. The study retrospectively compiled clinical, histopathological, and demographic characteristics, providing data on the challenges and delays in diagnosing scabies cases that mimic other dermatological conditions.
Results: In a cohort of 27 scabies cases with diagnostic challenges, the majority were females (63%) with a mean age of 64.8±15.9 years. Pre-admission, 66.7% of the patients used systemic antihistamines, 52.4% used immunosuppressants, and 42.9% used topical scabies treatment. Secondary xerosis cutis and contact dermatitis were noted in 11.1% and 7.4% of cases. The most frequently considered differential diagnoses by dermatologists included prurigo subacuta (29.6%), dermatitis herpetiformis (18.5 %), lymphomatoid papulosis (14.8%), and vesicular/bullous pemphigoid (11.1 %).
Conclusion: Previous treatments; prolonged infestations leading to severe secondary dermatological problems including prurigo subacuta, contact dermatitis, and xerotic eczema; atypical distribution of lesions; female prurigo; and elderly with prolonged pruritic atypical dermatoses or vesicular/bullous presentations should be kept in mind as clinical scenarios that may contribute to a delay in the diagnosis of scabies.