bluuebunny/crossref_metadata_2025_split huggingface dataset:
8‐oxoguanine lesioned B‐DNA molecule complexed with repair enzyme hOGG1: A molecular dynamics study
Authors: Pinak Miroslav 
Published: April 2003

AbstractThe molecular dynamics (MD) simulation of DNA mutagenic oxidative lesion, 7,8‐dihydro‐8‐oxoguanine (8‐oxoG), complexed with the repair enzyme, human oxoguanine glycosylase 1 (hOGG1), was performed for 1 nanosecond (ns) in order to describe the dynamical process of DNA‐enzyme complex formation. After 900 picoseconds of MD the lesioned DNA and enzyme formed a complex that lasted until the end of the simulation at 1 ns. The complex was mainly represented by the overlapping van der Waals surfaces of DNA and enzyme molecules. The amino group of arginine 324 was located close to the phosphodiester bond of the nucleotide with 8‐oxoG enabling chemical reactions between amino acid and lesion. The broken hydrogen bonds resulting in locally collapsed B‐DNA structure were observed at the lesion site. The phosphodiester bond at C5′ of 8‐oxoG was displaced to the position close to the amino group of arginine 324. The water‐mediated hydrogen bond network was formed in each contact area between DNA and enzyme, further enhancing the stability of the complex. In the background simulation of the identical molecular system with the native DNA, neither the complex nor the water‐ mediated hydrogen bond network was observed. © 2003 Wiley Periodicals, Inc. J Comput Chem 24: 898–907, 2003
Significance of serum IgA levels and serum IgA/C3 ratio in diagnostic analysis of patients with IgA nephropathy
Authors: Maeda Atsuko , Gohda Tomohito , Funabiki Kazuhiko , Horikoshi Satoshi , Shirato Isao , Tomino Yasuhiko 
Published: April 2003

AbstractDiagnostic analysis of clinical markers including serum IgA levels and serum IgA/C3 ratio in patients with IgA nephropathy is described. One hundred patients with IgA nephropathy (IgA nephropathy group) and 100 patients with other primary glomerular diseases (non‐IgA nephropathy group) were examined. The analysis was performed to distinguish between these two groups using four clinical markers: 1) more than five red blood cells in urinary sediments, 2) persistent proteinuria (urinary protein of more than 0.3 g/day), 3) serum IgA levels of more than 315 mg/dl, and 4) a serum IgA/C3 ratio of more than 3.01. Patients with three or four clinical markers were easily diagnosed as having IgA nephropathy in this study. Furthermore, there was a significant difference in these clinical markers between the good prognosis and relatively good prognosis groups (Groups I and II) and the relatively poor prognosis and poor prognosis groups (Groups III and IV) of IgA nephropathy patients. It appears that the presence of microscopic hematuria and/or persistent proteinuria, high serum IgA levels, and the serum IgA/C3 ratio are useful for distinguishing IgA nephropathy from other primary renal diseases. It is postulated that these clinical markers are also useful for diagnosis of IgA nephropathy without renal biopsy. J. Clin. Lab. Anal. 17:73–76, 2003. © 2003 Wiley‐Liss, Inc.
D‐dimer and its relationship to fibrinogen/fibrin degradation products (FDPs) in disorders associated with activation of coagulation or fibrinolytic systems
Authors: Moresco Rafael Noal , Vargas Luis Cláudio Rosa , Voegeli Carlos Franco , Santos Roberto Christ Vianna 
Published: April 2003

AbstractLevels of D‐dimer and fibrinogen/fibrin degradation products (FDPs) are significantly elevated in patients with deep venous thrombosis, pulmonary embolism, disseminated intravascular coagulation, or other complications. The diagnosis of these disorders can be difficult, time‐consuming, and expensive. Assays for the detection of FDPs and D‐dimer are used in many laboratories to investigate these disorders. The aim of this study was to compare plasma D‐dimer levels obtained by the immuno‐turdimetric method (Liatest D‐Dimer Diagnostica Stago, Asnières, France) with plasma FDPs were measured by latex agglutination to investigate the relationship between these parameters. These immunoassays were tested in 144 blood samples from patients with suspected disorders associated with activation of coagulation or fibrinolytic systems. D‐dimer and FDPs assays were performed in plasma samples by the use of Liatest D‐dimer and plasma FDPs (Diagnostica Stago), respectively. Patients were divided into four groups according to plasma D‐dimer levels. In group 1, 47.4% had negative FDPs and 52.6% had positive FDPs. In group 2, 15.4% had negative FDPs and 84.6% had positive FDPs. In group 3, 10.3% had negative FDPs and 89.7% had positive FDPs. All of the patients in group 4 had positive FDPs. It was shown that plasma D‐dimer levels measured by Liatest D‐dimer have a relation to FDP levels measured by latex agglutination. J. Clin. Lab. Anal. 17:77–79, 2003. © 2003 Wiley‐Liss, Inc.
Analysis of RHD genes in Taiwanese RhD‐negative donors by the multiplex PCR method
Authors: Lee Y.‐L. , Chiou H.‐L. , Hu S.‐N. , Wang L. 
Published: April 2003

AbstractThe determination of the RhD phenotype is important in transfusion medicine. However, due to the complexity of D antigen expression, the routine serological method cannot differentiate all RhD variants. In addition, the induction of the anti‐D antibody is still the major cause of severe hemolytic disease of the newborn (HDN). Therefore, it is important to understand RHD gene profiles. To analyze the RHD gene profiles of Taiwanese RhD‐negative donors, the multiplex PCR method was applied to amplify RHD specific exons 3, 4, 5, 7, and 9. Based on the PCR results, the 156 RhD‐negative donors were divided into 12 groups according to the different expression patterns of the RHD gene. These 12 groups were further divided into three categories: type I=Rh Del (21.8%); type II = partial D, containing some exons (9.0%); and type III = true RhD‐negative (69.2%). The results indicated that 21.8% of RhD‐negative donors in Taiwan were RhDel, and 9% carried a part of the RHD gene. Six defined RhD variants were found in this study: four ROHar, one DVa, and two DIVb. However, no true RhD‐negative or RhDel donor with the CcdEe phenotype was found in this analysis. J. Clin. Lab. Anal. 17:80–84, 2003. © 2003 Wiley‐Liss, Inc.
Expression of vascular endothelial growth factor in renal cell carcinoma is correlated with cancer advancement
Authors: Yang Ching‐Chiang , Chu Kang‐Chu , Yeh Wen‐Meng 
Published: April 2003

AbstractVascular endothelial growth factor (VEGF) functions as a regulator of neovascularization in malignant cells. VEGF as a mitogen is thought to alter renal cell carcinoma formation and tumor progression. We investigated the expression of the VEGF gene in order to evaluate its clinical significance in renal cell carcinoma. Tissue samples from 198 patients with renal cell carcinoma were examined with an immunohistochemical stain for the expression of the VEGF gene. The expression rate was compared to 34 normal renal cortical samples obtained from renal surgery from noncancer patients. There were significant differences between normal renal cortex (0%) and cancer tissue (54.5%) in positive staining of VEGF protein (P<0.001). With the progression of tumor grade, the positive rate of VEGF gene expression significantly increased. The expression rate of the VEGF gene in the advanced group, such as with lymph node involvement or vein invasion, was greater than that in the locally confined group (P<0.001). The results revealed that expression of the VEGF gene is proportional to the formation and progression of renal cell carcinoma, which may allow VEGF to be used as a prognostic marker for renal cell carcinoma. J. Clin. Lab. Anal. 17:85–89, 2003. © 2003 Wiley‐Liss, Inc.
Glucose‐6‐phosphate dehydrogenase deficiency does not result from mutations in the promoter region of the G6PD gene
Authors: Menounos Panayiotis G. , Garinis George A. , Patrinos George P. 
Published: April 2003

AbstractIn this study, we investigated whether glucose‐6‐phosphate dehydrogenase (G6PD) promoter mutations are responsible for G6PD deficiency. We analysed the G6PD proximal promoter and the 5′ untranslated region (UTR) in 65 G6PD‐deficient individuals, in which no mutations have been found in the G6PD gene coding sequences, using a nonradioactive polymerase chain reaction/single‐strand conformation polymorphism (PCR/SSCP) analysis. We identified no sequence variations in the G6PD core promoter or in the 5′ UTR of these G6PD‐deficient individuals, which indicates that G6PD deficiency is not associated with promoter mutations in the G6PD locus. J. Clin. Lab. Anal. 17:90–92, 2003. © 2003 Wiley‐Liss, Inc.
Gender‐related differences in hepatic activity of alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in humans
Authors: Chrostek Lech , Jelski Wojciech , Szmitkowski Maciej , Puchalski Zbigniew 
Published: April 2003

AbstractAlcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol metabolism in humans. Gender‐related differences in total liver ADH and ALDH activity among different animal species have been observed in many studies. We measured total ADH and ALDH activity, and the activity of class I–IV ADH in the livers of male and female patients. Total ADH and class I and II ADH activities were significantly higher in males than in females (P=0.0052, P=0.0074, P=0.020, respectively). Class III and IV ADH and total ALDH activities were not significantly different between the genders (P=0.2917, P=0.0590, P=0.2940, respectively). The results of our study clearly show that there is a difference in enzymatic activity between male and female patients for those isoenzymes that actively participate in ethanol oxidation in the liver (class I and II ADH), although the main form of ADH in this organ is class III ADH. J. Clin. Lab. Anal. 17:93–96, 2003. © 2003 Wiley‐Liss, Inc.
Development of a microplate ELISA for circulating E‐selectin: assay characterization, comparison with a commercial kit, wand establishment of normal reference values
Authors: Tsao Kuo‐Chien , Chang Pi‐Yueh , Li Chia‐Chi , Wu Tsu‐Lan , Sun Chien‐Feng , Wu James T. 
Published: April 2003

AbstractE‐selectin, an adhesion molecule, is detectable in the blood. Serum levels of E‐selectin can be a prognostic indicator for various malignancies. We developed a microplate enzyme‐linked immunosorbent assay (ELISA) for the detection of circulating E‐selectin by coating the microwell with monoclonal anti‐E‐selectin. We incubated the specimen and the biotinylated detecting antibody simultaneously, and used HPR‐conjugated streptavidin to generate a signal for quantification. The assay has a sensitivity of 3.8 ng/mL, and the coefficients of variation (CVs) for both within‐day and day‐to‐day precision were all <10%. Our in‐house‐made kit also compared well with a commercial kit from R&D Systems (r=0.95, slope=0.94). Using our kit we determined a normal reference value for Chinese individuals of different ages (30–80 years). We found no significant difference between females and males; however, age appeared to have an impact on the normal E‐selectin value. We found that normal individuals over 60 years old had higher levels of circulating E‐selectin (65.8±19.5 ng/mL, N=111) compared to those under 60 years old (59.5±18.1 ng/mL; P=0.002). Our kit appears to have sufficient sensitivity for detecting elevated circulating E‐selectin in various carcinomas. We believe that with the established normal reference value, our in‐house‐developed ELISA kit is well suited for routine clinical laboratory use. J. Clin. Lab. Anal. 17:97–101, 2003. © 2003 Wiley‐Liss, Inc.
Self‐disclosure in psychotherapy practice and supervision: An introduction
Authors: Farber Barry A. 
Published: April 2003

AbstractDisclosure in psychotherapy may be considered from multiple perspectives: therapist to patient, patient to therapist, trainee to supervisor, and supervisor to trainee. Individuals in each of these roles must decide if, when, and how they should articulate certain thoughts or feelings to their therapeutic counterpart. Multicultural considerations may well influence these decisions. The articles in this issue, including two that are research reviews, question the value and parameters of self‐disclosure in each of these contexts; they speak to the inevitable tension within psychotherapeutic dyads between the demands of open and honest disclosures and the equally potent countervailing forces of shame, tact, and appropriateness. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 525–528, 2003.
Therapist self‐disclosure: Research‐based suggestions for practitioners
Authors: Knox Sarah , Hill Clara E. 
Published: April 2003

AbstractAfter defining therapist self‐disclosure, we briefly discuss its theoretical foundations. We then move to our primary focus of presenting a number of suggestions for effective use of therapist self‐disclosure, based on the extant empirical literature. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 529–539, 2003.
Self‐disclosure in psychoanalytic–existential therapy
Authors: Geller Jesse D. 
Published: April 2003

AbstractThis article is an effort to integrate contemporary psychoanalytic and existential perspectives on intentional therapist self‐disclosure. It offers a two‐stage decision‐making model that considers self‐disclosure from the vantage points of style and internalization. Clinical and research findings are presented to support the notion that the meanings a patient attributes to a particular self‐disclosure, and its power to move him or her towards greater health, is the product of a fluctuating matrix of interpersonal and intrapsychic variables. Special consideration is given to the challenges that arise during the early and termination stages of treatment and to the psychotherapy of therapists. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 541–554, 2003.
Therapist self‐disclosure in cognitive‐behavior therapy
Authors: Goldfried Marvin R. , Burckell Lisa A. , Eubanks‐Carter Catherine 
Published: April 2003

AbstractAlthough cognitive‐behavior therapy emphasizes between‐session change, therapist self‐disclosure within the session can be an effective tool for strengthening the therapeutic bond and facilitating client change. After noting the use of self‐disclosure in other theoretical orientations, we place self‐disclosure in the context of cognitive‐behavioral theories of reinforcement and modeling. Clinical vignettes illustrate the use of therapist self‐disclosure to provide feedback on the interpersonal impact made by the client, enhance positive expectations and motivation, strengthen the therapeutic bond, normalize the client's reaction, reduce the client's fears, and model an effective way of functioning. Therapists need to observe appropriate boundaries when self‐disclosing, and in particular, should consider their own motivations for self‐disclosing. Although more research is needed on the effects of self‐disclosure, cognitive‐behavior therapists have found that self‐disclosure can be a powerful intervention. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 555–568, 2003.
Cross‐cultural considerations of therapist self‐disclosure
Authors: Constantine Madonna G. , Kwan Kwong‐Liem Karl 
Published: April 2003

AbstractMuch of the existing literature on psychotherapist self‐disclosure has been with White or European American individuals. This article discusses cross‐cultural considerations related to therapist self‐disclosure. We present a clinical example of how therapist self‐disclosure might play an important role with regard to working with people of color. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 581–588, 2003.
Patient self‐disclosure: A review of the research
Authors: Farber Barry A. 
Published: April 2003

AbstractResearch shows that whereas most patients disclose deeply personal experiences in therapy, a significant proportion conceal some significant information. Findings also indicate that there are several categories of nondisclosed information (secrets, things left unsaid, and client reactions); that patients tend to withhold immediately experienced negative reactions; that disliked characteristics of oneself and parents are among the most thoroughly discussed issues in therapy while sex, aggression, and personal failure are least discussed; that men and women disclose to the same extent and on similar topics; that shame inhibits disclosure of negative affect; that a strong therapeutic alliance, overall tendency to be disclosing, and time in therapy facilitate disclosure; and that the discrepancy between disclosure and patients' ratings of salience of disclosure is a more powerful predictor of outcome than disclosure alone. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 589–600, 2003.
Trainee disclosure in psychotherapy supervision: The impact of shame
Authors: Yourman David B. 
Published: April 2003

AbstractResearch indicates that psychotherapy trainees often withhold information from supervisors even though they are expected to be self‐disclosing in the supervisory process. A contributing factor to this nondisclosure is trainee shame. By its very nature, psychotherapy supervision is an endeavor in which trainees are likely to experience feelings of self‐doubt and shame. Because shame is an affect that often provokes a desire to hide oneself, it follows that supervisees experiencing more shame will be less likely to be forthcoming, especially about material that might be viewed negatively by their supervisors. The material most often withheld by trainees pertains to problems within the supervisory relationship. It appears that trainee shame and nondisclosure have the greatest impact upon the quality of the psychotherapy supervision itself, as opposed to the treatment being supervised. Four examples of supervisory dyads affected by trainee shame and nondisclosure are presented. Each case is examined in terms of what could have triggered the supervisee's shame, the consequences of the disrupted communications, and ways in which the situation might have been improved. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 601–609, 2003.
Therapist self‐disclosure with children, adolescents, and their parents
Authors: Gaines Robert 
Published: April 2003

AbstractMany therapists who work with children and adolescents make extensive use of self‐disclosure. However, these interventions have received little attention in the literature, and the basis for using them has not been well established. A developmental/relational perspective on the therapeutic process provides a cogent foundation using therapist self‐disclosure with children, adolescents, and their parents. Therapist self‐disclosure facilitates the negotiation of many important therapeutic tasks. Clinicians working with children and adolescents almost always work concurrently with parents, yet the nature of this work has not been clearly conceptualized. I advance a collaborative model for parent work, highlighting the essential role of therapist self‐disclosure. Extensive clinical examples of therapist self‐disclosure with children, adolescents, and their parents are provided. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 569–580, 2003.
Supervisor self‐disclosure: Balancing the uncontrollable narcissist with the indomitable altruist
Authors: Ladany Nicholas , Walker Jessica A. 
Published: April 2003

AbstractThe purpose of this article is to provide supervisors with a framework to determine the effectiveness of self‐disclosure in supervision. We posit how self‐disclosures can be both memorable to the trainee and facilitative of supervision process and outcome, specifically the supervisory working alliance, trainee disclosure, and trainee edification. Case examples based on the literature and our own personal experiences are offered to illustrate the models' applicability. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 611–621, 2003.
The many faces of self‐disclosure
Authors: Stricker George 
Published: April 2003

AbstractThe contributions of eight authors to this issue of In Session are discussed, with emphasis on who discloses what to whom, in what ways, and for what reasons. The primary conclusions concern the varieties of self‐disclosure, the motives for self‐disclosure, the appropriate outcome variable in studying self‐disclosure, the value of evidence, the importance of the context, and the crucial role of the relationship. Self‐disclosure, properly used, can be a significant contributor to successful psychotherapy. © 2003 Wiley Periodicals, Inc. J Clin Psychol/In Session 59: 623–630, 2003.
Primary ascaridial perforation of the small intestine: Sonographic diagnosis
Authors: Chawla Ashish , Patwardhan Vasudha , Maheshwari Mohit , Wasnik Ashish 
Published: April 2003

AbstractAscaris lumbricoides is the most common helminth affecting humans. Ascariasis can result in serious complications, including intestinal obstruction and perforation. Early diagnosis and treatment of such complications reduces the risk of mortality. We present a case of sonographically diagnosed ascaridial perforation in a 5‐year‐old girl. On sonography, each ascarid appeared as 2 pairs of parallel lines, representing the worm's outer margins, flanking a central sonolucent line, representing its digestive tract. Sonography revealed ascarides in the peritoneal cavity and in some loops of the small bowel. Emergent laparotomy was performed to remove ascarides from the peritoneal cavity and terminal ileum, and the patient recovered well and was asymptomatic at a 3‐month follow‐up. Knowledge of the sonographic features described herein may aid in the evaluation of patients, especially children, in tropical countries who have clinical symptoms of ascariasis. © 2003 Wiley Periodicals, Inc. 31:211–213, 2003
Suspicion of microscopic colitis raised by sonographic examination
Authors: Hollerweger Alois , Macheiner Peter , Brunner Walter 
Published: April 2003

AbstractThis report describes the case of a 57‐year‐old woman who was incidentally identified as having lymphocytic colitis after she underwent routine transabdominal sonographic examination. She initially reported having no irregularities in her bowel movements. Sonography revealed the following nonspecific findings: watery stool in the entire colon, slight thickening of the hypoechoic mucosal layer and moderate thickening of the hyperechoic submucosal layer of the colon, and no pathologic findings in the small intestine. On additional questioning, the patient said that she had had watery diarrhea for the last 10 years, with as many as 10 bowel movements daily. Endoscopic examination and biopsy were performed. Histopathologic examination of biopsy specimens showed lymphocytic infiltration of the mucosa and some collagen deposits, consistent with a diagnosis of lymphocytic colitis. Treatment was begun with loperamide, sulfasalazine, and budesonide. Within 3 weeks of the start of treatment, the number of bowel movements decreased to 1–2 daily. Follow‐up sonography at that time revealed normalization of the bowel contents and disappearance of the thickened submucosal layer of the colon. Nonspecific sonographic findings like those in this case lead to the need to rule out various diseases through further appropriate evaluations to identify the correct diagnosis. © 2003 Wiley Periodicals, Inc. 31:207–210, 2003