bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of piperacillin‐tazobactam in the treatment of surgical wound infection after clean‐contaminated head and neck oncologic surgery
Authors: Rodrigo Juan P. , Sŭrez Carlos , Bernaldez Ricardo , Collado Diego 
Published: July 2004

AbstractBackground.Although perioperative antibiotic prophylaxis has significantly reduced surgical wound infection rates, this complication is still a frequent complication of head and neck cancer surgery. Because these infections are typically polymicrobial, our study evaluated the safety and efficacy of piperacillin‐tazobactam in the treatment of surgical wound infection after clean‐contaminated head and neck oncologic surgery.Methods.In this multicenter, prospective clinical trial, 70 patients with surgical wound infection received piperacillin‐tazobactam.Results.Of patients who were evaluable, 92.4% were also clinically cured or improved, and the bacteriologic eradication rate was 80.3%. Of the 70 patients enrolled in the study, six (8.5%) experienced six adverse events: two cases of moderate diarrhea, one allergic skin reaction, and three cases of phlebitis. No deaths were attributable to the study drug.Conclusions.Piperacillin‐tazobactam is a good choice of treatment as monotherapy for surgical wound infection after clean‐contaminated head and neck oncologic surgery. © 2004 Wiley Periodicals, Inc. Head Neck 26: 823–828, 2004
Mutation of p53 in head and neck squamous cell carcinoma correlates with Bcl‐2 expression and increased susceptibility to cisplatin‐induced apoptosis
Authors: Andrews Genevieve A. , Xi Sichuan , Pomerantz Rebecca G. , Lin Charles J. , Gooding William E. , Wentzel Abbey L. , Wu Li , Sidransky David , Grandis Jennifer Rubin 
Published: July 2004

AbstractBackground.The p53 protein, a well‐known tumor suppressor that functions primarily as a transcription factor, initiates cell cycle arrest and apoptosis after genotoxic stress. The antiapoptotic regulator Bcl‐2 is a downstream modulator of p53‐induced apoptosis. Loss of function of the p53 tumor suppressor through mutation is an important event that contributes to cellular transformation. Mutation of p53 is one of the most common genetic alterations in squamous cell carcinomas of the head and neck (SCCHN). We hypothesized that p53 mutation is associated with Bcl‐2 expression and susceptibility to apoptosis in SCCHN.Methods.Exons 5 to 8 of the p53 gene were sequenced in 22 SCCHN tumor samples and correlated with the Bcl‐2 expression and apoptosis rates in these tumors. In addition, a Bcl‐2–expressing SCCHN cell line, UMSCC74B, was stably transfected with a temperature‐sensitive mutant p53 construct, and Bcl‐2 expression levels were examined at the mutant and the wild‐type temperatures.Results.Bcl‐2 expression was inversely correlated with wild‐type p53 status in SCCHN tumors (p = .05). Furthermore, there was a modest increase (1.7‐fold) in apoptosis in the wild‐type p53 tumors compared with mutant p53 SCCHN. Immunoblotting of UMSCC74B cells stably transfected with the temperature‐sensitive mutant p53 construct demonstrated that shifting these cells to the mutant p53 temperature (39.5°C) resulted in decreased expression of Bcl‐2 compared with levels in cells grown at the wild‐type p53 temperature (32.5°C). Further investigation showed that SCCHN cells expressing predominantly mutant p53 and decreased Bcl‐2 were more susceptible to cisplatin‐induced apoptosis than vector‐transfected controls (p < .0001).Conclusions.These results suggest that p53 mutation directly modulates Bcl‐2 expression and therefore susceptibility to chemotherapy‐induced apoptosis in SCCHN cells in vitro. © 2004 Wiley Periodicals, Inc. Head Neck 26: 870–877, 2004
Concomitant radiochemotherapy in primary inoperable advanced head and neck cancer with 5‐fluorouracil and mitomycin‐C
Authors: Christiansen Hans , Hermann Robert M. , Hille Andrea , Weiss Elisabeth , Nitsche Mirko , Martin Alexios , Hess Clemens F. , Pradier Olivier 
Published: July 2004

AbstractBackground.The purpose of this study was to evaluate the efficacy and toxicity of radiotherapy and concomitant 5‐fluorouracil (5‐FU) and mitomycin‐C infusion in inoperable head and neck cancer.Methods.Seventy‐six patients (86% men, 14% women), mean age 57 years, with primary inoperable head and neck cancer were treated with 70 Gy plus simultaneous intravenous chemotherapy with 5‐FU (600 mg/m2/d, days 1 to 5) and mitomycin‐C (10 mg/m2, day 5 plus 36).Results.After a mean follow‐up of 13 months, 31 patients were alive. Complete response (CR) was seen in 63%. The 1‐ and 2‐year overall survival rates were 67.7% and 39.5%, and locoregional control rates were 51.7% and 35.6%. Pretreatment hemoglobin <13.9 g/dL was associated with lower locoregional control rates (p = .03). Therapy was well tolerated (grade 3 mucositis in 21%, grade 4 in 1%, grade 3 leukopenia in 11%).Conclusions.Our radiochemotherapy regimen offers a curative option for this group of patients with a poor prognosis. Hemoglobin levels before therapy have an influence on prognosis. © 2004 Wiley Periodicals, Inc. Head Neck 26: 845–853, 2004
Cutaneous metastatic squamous cell carcinoma to the parotid gland: analysis and outcome
Authors: Audet Nathalie , Palme Carsten E. , Gullane Patrick J. , Gilbert Ralph W. , Brown Dale H. , Irish Jonathan , Neligan Peter 
Published: July 2004

AbstractBackground.Our aim was to review the presentation, treatment, and outcome of patients with metastatic cutaneous squamous cell carcinoma involving the parotid gland at a tertiary referral center.Methods.We performed a retrospective chart review of the cancer registry at the Princess Margaret Hospital, Toronto, from 1970 to 2001. All patients had a previously untreated metastatic cutaneous head and neck squamous cell carcinoma involving the parotid gland. A minimal follow‐up of 1 year was mandatory for inclusion in the study.Results.Fifty‐six white patients (43 men and 13 women), with a median age of 76 years (range, 49–97 years), were eligible for inclusion. The disease in all patients was retrospectively staged according to a new system. Twenty patients had P1 disease, 14 had P2, and 22 had P3. Therapy included surgery and adjuvant external beam radiation in 37 patients, single‐modality external beam radiation in 12, and surgery alone in seven patients. The overall recurrence rate was 29%. The disease‐specific survival was significantly worse in patients treated with external beam radiation alone (p < .05). Tumor size >6 cm (p < .01) and the presence of facial nerve involvement (p < .01) were poor prognostic factors.Conclusions.Metastatic cutaneous squamous cell carcinoma to the parotid gland is an aggressive neoplasm that requires combination therapy. The presence of a lesion in excess of 6 cm or with facial nerve involvement is associated with a poor prognosis. © 2004 Wiley Periodicals, Inc. Head Neck 26: 727–732, 2004
Parathyroid carcinoma: A 22‐year experience
Authors: Busaidy Naifa L. , Jimenez Camilo , Habra Mouhammed Amir , Schultz Pamela N. , El‐Naggar Adel K. , Clayman Gary L. , Asper Joshua A. , Diaz Eduardo M. , Evans Douglas B. , Gagel Robert F. , Garden Adam , Hoff Ana O. , Lee Jeffrey E. , Morrison William H. , Rosenthal David I. , Sherman Steven I. , Sturgis Erich M. , Waguespack Steven G. , Weber Randal S. , Wirfel Kelly , Vassilopoulou‐Sellin Rena 
Published: July 2004

AbstractPurpose.Because parathyroid carcinoma is rare, clear consensus is not available regarding the optimal management of patients with this condition. Treatment strategies generally derive from clinical and anecdotal experiences. We report our experience with this entity.Methods.We included all patients with parathyroid carcinoma seen at The University of Texas M. D. Anderson Cancer Center since January 1, 1980. The medical records and pathology specimens were reviewed and verified in all cases.Results.Since 1980, 27 patients (16 men and 11 women) registered at M. D. Anderson Cancer Center with parathyroid carcinoma and a minimum follow‐up of 2 years. The age at initial diagnosis (mean ± SD) was 46.7 ± 15.3 years. All patients were seen with hypercalcemia (mean calcium, 13.4 ± 1.5 mg/dL). Eighteen patients had locally invasive disease, eight had localized disease, and one had distant metastasis. Parathyroid cancer was treated with complete surgical excision with curative intent in 18 patients. In the other nine patients, who had clinical and/or radiographic evidence of soft tissue extension, the tumor was treated by comprehensive “en bloc” soft tissue resection. Of six patients who received adjuvant radiotherapy after initial surgery, only one had a local relapse. In contrast, of 20 patients who did not receive adjuvant radiotherapy, 10 had a local relapse, excluding the one patient who had distant metastases. The 5‐year survival was 85%, and the 10‐year survival was 77%. Five patients died of parathyroid carcinoma; all deaths were hypercalcemia related.Conclusions.Parathyroid carcinoma can be an indolent disease with morbidity and mortality related to hypercalcemia. Adjuvant radiotherapy may improve local control and limit the occurrence of local relapse. A comprehensive multidisciplinary approach with surgery, radiation therapy, and medical treatment for hypercalcemia is needed to optimize patient outcome. © 2004 Wiley Periodicals, Inc. Head Neck 26: 716–726, 2004
Supraglottic hemipharyngolaryngectomy for the treatment of T1 and T2 carcinomas of laryngeal margin and piriform sinus
Authors: Makeieff Marc , Mercante Guiseppe , Jouzdani Elhám , Garrel Renaud , Crampette Louis , Guerrier Bernard 
Published: July 2004

AbstractBackground.We evaluated the functional and oncologic results of supraglottic hemipharyngolaryngectomy as treatment for T1 and T2 lateral laryngeal margin and piriform sinus carcinomas.Methods.Eighty‐seven patients underwent this surgical treatment. The disease was classified T1 in 14 of these cases (16.1%) and T2 in 73 cases (83.9%). The nodal status indicated 39 cases of N0 (44.8%), 18 cases of N1 (20.7%), 28 cases of N2 (32.2%), and two cases of N3 (2.2%). With regard to the N0 cases, 15 (38.4%) were positive at the histologic examination. Within the N+ group, 52.1% involved capsular rupture.Results.Two patients died of complications during the postoperative period. The mean duration of nasogastric tube feeding was 20 days. Six patients (7.27%) had feeding resumption problems. All patients were decanulated after a mean period of 16 days. All patients underwent postoperative radiation therapy, except two with T1N0N− disease and three who had previously undergone this treatment. The 5‐year actuarial survival rate was 60.3% (T1, 83.3%; T2, 49.9%). The rates of local and regional recurrence, second primary cancer, and metastasis were 19.5%, 24.1%, and 28.1%, respectively. The infringement of the pharyngoepiglottic fold was significantly correlated with locoregional recurrence. The survival rate was significantly correlated with the nodal status and extracapsular spread.Conclusions.Initial staged cancers of the laryngeal margin and piriform sinus can be successfully managed with conservative surgery called supraglottic hemipharyngolaryngectomy combined with nodal neck dissection. Postoperative radiation therapy is still recommended in most cases because of the high recurrence potential and prevalence of secondary regional cancers. This combined treatment seems to be a suitable therapeutic choice in the treatment of patients with T1 and T2 carcinomas of the laryngeal margins and piriform sinus. © 2004 Wiley Periodicals, Inc. Head Neck 26: 701–705, 2004
Identification of an HLA‐DR‐restricted peptide epitope with a promiscuous binding pattern derived from the cancer testis antigen HOM‐MEL‐40/SSX2
Authors: Neumann Frank , Wagner Claudia , Stevanovic Stefan , Kubuschok Boris , Schormann Claudia , Mischo Axel , Ertan Kubilay , Schmidt Werner , Pfreundschuh Michael 
Published: July 2004

AbstractThe SSX2 gene encodes the tumor‐specific antigen HOM‐MEL‐40/SSX2 expressed in a broad spectrum of tumors of different origin, against which humoral and CD8+ T‐cell‐mediated MHC‐I‐restricted responses have been demonstrated. Searching for promiscuous MHC‐II‐restricted peptides that might be suitable as a CD4+ stimulating vaccine for many patients, we used the SYFPEITHI algorithm and identified a HOM‐MEL‐40/SSX2‐derived pentadecamer epitope (p45–59) that induced specific CD4+ T‐cell responses restricted by the HLA‐DRB1 subtypes *0701, *1101 and *1302 that have a cumulative prevalence of ∼ 25% in the Caucasian population. The CD4+‐mediated response against p45–59 and its DR restriction was demonstrated by inhibition with anti‐CD4 and HLA‐DR antibodies, respectively, and by blocking experiments using HLA‐specific antibodies. The natural processing and presentation of p45–59 was demonstrated by recognition of the SSX2+ melanoma cell line Me 275 as well as autologous and allogeneic dendritic cells pulsed with whole‐protein SSX2 by T cells with specificity for p45–59. p45–59 was able to induce responses in 3/6 breast cancer patients and 1/5 healthy controls. No correlation was found between CD4+ T‐cell responses against p45–59 reactivity and anti‐SSX2 antibody titers in the serum of patients, suggesting that CD4+ and B‐cell responses are regulated independently. p45–59 holds promise as a broadly applicable peptide vaccine for patients with SSX2‐positive neoplasms. © 2004 Wiley‐Liss, Inc.
Retinol, carotenoids and the risk of prostate cancer: A case‐control study from Italy
Authors: Bosetti Cristina , Talamini Renato , Montella Maurizio , Negri Eva , Conti Ettore , Franceschi Silvia , La Vecchia Carlo 
Published: July 2004

AbstractSeveral studies have evaluated the possible association between intakes of retinoids and carotenoids and the risk of prostate cancer, but the evidence is still inconsistent. Further, only a few studies have investigated the role of specific carotenoids other than β‐carotene. We have thus considered the association between retinol and various carotenoids using data from a multicentric case‐control study conducted in Italy between 1991 and 2002. This included 1,294 incident, histologically confirmed prostate cancer cases below age 75 years admitted to major teaching and general hospitals in the areas under study, and 1,451 controls below age 75 years selected among patients admitted to the same hospitals as cases for a wide spectrum of acute nonneoplastic conditions not related to long‐term modifications of diet. Subjects' usual diet was investigated by means of a validated food‐frequency questionnaire. Multivariate odds ratios and the corresponding 95% confidence intervals were estimated using unconditional logistic regression models. The risk of prostate cancer tended to decrease with increasing intake of retinol (OR = 0.79 for the highest versus the lowest quintile of intake), carotene (OR = 0.70), α‐carotene (OR = 0.85) and β‐carotene (OR = 0.72), although the estimates were significant for carotene and β‐carotene only. No meaningful associations emerged for nonprovitamin A carotenoids, such as lycopene (OR = 0.94) and lutein/zeaxanthin (OR = 0.91). No systematic heterogeneity was observed across strata of age, education and body mass index. Thus, our study supports the hypothesis of a weak protective effect of carotene, particularly β‐carotene, on the risk of prostate cancer, while it indicates that other carotenoids, including lycopene, and retinol are not appreciably related to the risk of this neoplasm. © 2004 Wiley‐Liss, Inc.
Texture and distribution of pectic substances of mango as affected by infusion of pectinmethylesterase and calcium
Authors: Banjongsinsiri Panida , Kenney Stephen , Wicker Louise 
Published: July 2004

AbstractFresh cut mangos were infused with pectinesterase (PME) and calcium chloride, and the effect on textural properties, distribution of pectic substance and degree of esterification was determined. Temperature gradient infusion with PME and/or calcium chloride increased gumminess and chewiness, but had no impact on hardness and adhesiveness. The distribution of pectic substances, as protopectin or alkaline soluble pectin, was approximately twice that of water‐ or chelator‐soluble pectin. The degree of esterification of water‐ and chelator‐soluble pectic substances was near 50–60%, and less than 10%, respectively. Heat‐sensitive PME inhibitor in mango was detected. The initial hardness of Kent mango was variable, and differences in distribution of pectic substances were observed. Texture of Kent mango is most likely moderated by changes in the solubility of insoluble pectin or by non‐pectin components in the cell wall. Copyright © 2004 Society of Chemical Industry
Bioavailability of iron from a heat treated glucose‐lysine model food system: assays in rats and in Caco‐2 cells
Authors: Delgado‐Andrade Cristina , Seiquer Isabel , Navarro M Pilar 
Published: July 2004

AbstractThe influence of Maillard reaction products (MRP) from glucose–lysine on iron bioavailability was investigated. Equimolar glucose–lysine mixtures (GL, 40% moisture) were heated (150 °C, for 30, 60 and 90 min) to prepare samples GL30, GL60 and GL90, respectively, and were used to study the influence of MRP on iron solubility, iron uptake and transport in Caco‐2 cells and iron balance in rats. After an initial increase with GL30, in vitro iron solubility decreased in the presence of most of the heated samples. The Caco‐2 cell experiments showed that Fe cell content increased in the presence of heated mixtures with respect to GL, whereas transport was less affected. Iron balance was determined in rats fed diets containing the GL30 and GL90 samples (3%) for a 21 day period and compared with that measured in a control group. Consumption of glucose–lysine heated mixtures increased total iron retention, although hemoglobin values decreased. Iron accumulations in organs such as the spleen, kidney and small intestine were observed. Copyright © 2004 Society of Chemical Industry
Developing wine trails as a tourist attraction in Israel
Authors: Jaffe Eugene , Pasternak Hanoch 
Published: July 2004

AbstractThe objective of this paper is to suggest marketing strategies for the development of wine trails in Israel as tourist attractions. Both inbound and domestic tourists were surveyed as to their motives for visiting wineries and their experiences from those visits. The expectations of tourists with regard to the wine trail experience were compared with the beliefs of winery operators. The findings showed that there was a high degree of understanding of tourist needs among the winery operators. Nevertheless, augmentation of the winery experience is necessary if wine trail tourism is to further develop. Copyright © 2004 John Wiley & Sons, Ltd.
Madrid as a tourist destination: analysis and modelization of inbound tourism
Authors: Garín‐Muñoz Teresa 
Published: July 2004

AbstractThis paper presents a model for explaining the international tourism flows to Madrid. The study is based primarily on examining the main characteristics of inbound tourism flows to Madrid in terms of its structure, evolution and composition by countries of origin of the visitors. According to the general principles of economic theory and the classic literature on tourism demand, a description of the main factors to be considered in determining tourism flows is presented. In the second part of the paper, by using data corresponding to 25 countries of origin and 16 years (1985–2000), an econometric (panel data) model is used to estimate the quantitative relationship between the level of inbound tourism to Madrid and its determinants. The model selected has been subject to a battery of diagnostics in order to test the validity of the assumptions that underlie the estimation. By using the Im–Pesaran–Shin test for unit roots the null hypothesis of non‐stationarity of residuals is rejected and it is concluded that the model can be used for inference. The results show that income of the countries of origin of the travellers and costs of living of the tourists in Madrid relative to the costs of living in the origin country were among the most important factors of the international tourism demand. An interpretation of the results in terms of elasticities is presented in the last part of the paper, as well as their implications for tourism policy. Copyright © 2004 John Wiley & Sons, Ltd.
Sway patterns in a case of orthostatic tremor responsive to alcohol
Authors: Bacsi Ann M. , Halmagyi G. Michael , Colebatch James G. 
Published: July 2004

AbstractWe describe changes in the extent of sway in a man with orthostatic tremor (OT) who reported increased stability after alcohol. He was tested at baseline and again after 40 g (0.5 g/kg) of alcohol. These results were compared to those of 3 age‐matched controls (no alcohol). The patient's baseline sway was greater than controls, larger in the lateral than the anteroposterior plane, but retained normal responsiveness to the use of external support, increasing stance width, and vision. Tremor frequency significantly decreased after alcohol, as did low‐ and high‐frequency tremor amplitude and the extent of body sway. Despite these findings, sway remained greater than controls. OT thus may show functionally important alcohol responsiveness. © 2004 Movement Disorder Society
Effect of stimulation frequency on tremor suppression in essential tremor
Authors: Ushe Mwiza , Mink Jonathan W. , Revilla Fredy J. , Wernle Angie , Schneider Gibson Patricia , McGee‐Minnich Lori , Hong Minna , Rich Keith M. , Lyons Kelly E. , Pahwa Rajesh , Perlmutter Joel S. 
Published: July 2004

AbstractWe sought to determine the effect of deep brain stimulation (DBS) frequency on tremor suppression in essential tremor (ET) patients with deep brain stimulators implanted in the ventral intermediate nucleus (VIM) of the thalamus. A uniaxial accelerometer was used to measure tremor in the right upper extremity of subjects with a diagnosis of ET who had DBS electrodes implanted in the left VIM. The root‐mean‐square acceleration was used as the index of tremor magnitude and normalized to the OFF DBS condition. There was a highly significant inverse sigmoidal relationship between stimulation frequency and normalized tremor acceleration (X2/DoF = 0.42, r2 = 0.997). Tremor acceleration had a nearly linear response to stimulation frequencies between 45 and 100 Hz with little additional benefit above 100 Hz. These findings have two important implications. Clinically, frequency of thalamic stimulation is an important variable for optimal tremor control with maximal benefit achieved with 100 to 130 Hz in most patients. Second, thalamic DBS provides tremor benefit in a graded manner and is not an all‐or‐nothing phenomenon. © 2004 Movement Disorder Society