bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioresource collection of blood components from pregnant women to identify fetal genetic features, as well as to search for genetic markers of gestational complications
Authors: Vashukova E. S. , Tarasenko O. A. , Talantova O. E. , Kozyulina P. Yu. , Morshneva A. V. , Maltseva А. R. , Pachulia O. V. , Bespalova O. N. , Kogan I. Yu. , Glotov A. S. 
Published: December 2023

Aim. To create collection of plasma and leukocyte blood samples from pregnant women to improve methods of non-invasive prenatal testing, as well as to search for deoxyribonucleic acid markers and study the molecular mechanisms of gestational complications.Material and methods. The collection creation process uses original standard operating procedures. The biomaterial for storage was plasma and leukocytes from the blood of pregnant women.Results. As of July 2023, the collection contains samples of blood plasma and buffy coat suspension from 5814 pregnant women. For 5692 women, whole- genome sequencing of extracellular deoxyribonucleic acid from blood plasma was performed on an Ion GeneStudio S5 system (Thermo Fisher Scientific Inc, USA). Data on cytogenetic examination of fetal material after invasive prenatal diagnosis were collected from 522 women. In 241 cases, samples were obtained from pregnant women with fetuses with a normal karyotype and in 282 cases from pregnant women with fetuses with chromosomal abnormalities. After pregnancy ended with childbirth, data on the course and outcomes of pregnancy were collected from 590 women. In 212 cases, pregnancy was complicated by great obstetrical syndromes. Based on the collection, a previously developed bioinformatic algorithm for non-invasive prenatal analysis was tested on a DNBSEQ-G50 sequencer (MGI, China). The obtained specificity corresponded to the expected one and was >99,9%. The sensitivity of the method is 100%.Conclusion. The created collection, as well as the clinical and genomic data associated with it, is a valuable resource for basic and applied research.
Genogeographic technologies of a population biobank as a tool for assessing selection effects (using the example of pharmacogenetic biomarkers of cardiovascular diseases)
Authors: Balanovskaya E. V. , Gorin I. O. , Ponomarev G. Yu. , Pylev V. Yu. , Belov R. O. , Pocheshkhova E. A. , Abdullaev Sh. P. , Mirzaev K. B. , Sychev D. A. 
Published: December 2023

Significant differences between the gene pools of Russian peoples require the development of ethno- regional adapted pharmacogenetic tests and the identification of priority regions for their implementation.Aim. To develop a genogeographic technology to identify selection effects using the example of biomarkers that are significant for pharmacotherapy of patients with cardiovascular diseases (CVD), using a population biobank and the Pharmacogenetics of Populations of Russia and Adjacent Countries database.Material and methods. Deoxyribonucleic acid (DNA) samples from the Biobank of Northern Eurasia from 20 metapopulations of the indigenous population of the European Russia were studied using two following data sets: 24 pharmacogenetic markers of CVDs (3170 samples); 1 276 191 polymorphic DNA markers of the autosomal genome (1293 samples). For each data set, estimates of interpopulation variability in the gene pool are provided — the difference between these estimates characterizes the selection pressure on each of the 24 CVD biomarkers. A genogeographic atlas has been created, the maps of which demonstrate the selection pressure on each biomarker according to the degree of deviation from the selective- neutral variability of the gene pool.Results. Twenty-four CVD biomarkers are divided into three following classes: those close to selective- neutral variability, those subject to stabilizing and differentiating selection. For each of the 24 CVD biomarkers, genogeographic maps were created that reveal selection effects in each of the 20 metapopulations. Most maps have identified populations that are under differential selection pressure and therefore a priority for the implementation of ethno- regionally adapted pharmacogenetic protocols.Conclusion. Pharmacogenetic markers and populations under differential selection require the development of ethno- regionally adapted pharmacogenetic tests. The created cartographic atlas of selection can serve as the basis for pharmacogenetic studies carried out using genogeographic methods.
Abordando la brecha de publicaciones en los Andes Tropicales: revelando desafíos e implementando soluciones
Authors: Valdez Jose , Vergara Lucía Castro , Orihuela Gabriela , Fernandez Miguel 
Published: December 2023

El aporte de la investigación sobre la conservación de la biodiversidad en el avance del conocimiento y la respuesta a los desafíos globales es fundamental. Sin embargo, aún existe una brecha sorprendente entre los Andes Tropicales y otras regiones en relación a la publicación de investigaciones científicas. Para comprender esta disparidad, se empleó una estrategia integral, que abarcó un taller de tres días destinado a abordar los principales desafíos que enfrentan los investigadores en la región de los Andes Tropicales, así como una serie de encuestas. La encuesta de inscripción, distribuida a más de 500 participantes potenciales, examinó la demografía, las percepciones de los factores que impactan las tasas de publicación más bajas, las barreras personales para la publicación y propuso estrategias para superar estos obstáculos. Esta fue seguida por una encuesta previa al taller, en la que se profundizó en los intereses específicos de los participantes seleccionados, los obstáculos que pretendían superar y sus preferencias por el contenido del taller. Por último, la encuesta a posteriori, en la que se evaluó el impacto del taller, la satisfacción de los participantes, los temas de interés en las sesiones y las recomendaciones para futuros talleres. La encuesta descubrió varios desafíos, como la capacitación limitada en publicaciones, factores culturales, barreras idiomáticas, recursos restringidos y limitaciones financieras, todos los cuales contribuyen a la brecha en las publicaciones. Sorprendentemente, el interés en el taller provino principalmente de profesionales experimentados, lo que indica que el desafío no se debe a la falta de habilidades sino más bien a la lucha por convertir su experiencia en un trabajo publicado. Además, alrededor de dos tercios de los participantes tenían materiales listos para publicar, lo que mostraba una reserva de conocimientos sin explotar. Una barrera importante identificada fue la limitada comprensión del proceso de publicación, superando incluso a la barrera del idioma, destacando la necesidad de aclarar este proceso. Otros hallazgos subrayan el papel fundamental de la confianza en uno mismo, siendo las dudas sobre uno mismo, el miedo al rechazo y los problemas de confianza las principales limitaciones personales. El estudio destaca la importancia de abordar los desafíos que enfrentan los profesionales experimentados en los Andes Tropicales al comprender las necesidades individuales, fomentar el apoyo y desmitificar el proceso de publicación. Esto ofrece un camino prometedor para cerrar la brecha de publicaciones y lograr que la ciencia producida en esta región se documente y amplifique aportando al conocimiento que ayude a preservar la rica biodiversidad de esta región.
O horizonte valorativo em enunciados do gênero comentário online na defesa de um currículo homeschooling: entre o conservadorismo e o neoliberalismo
Authors: Simões Ana Beatriz 
Published: December 2023

Este trabalho, em diálogo com os pressupostos teórico-metodológicos da Perspectiva Dialógica da Linguagem, elaborada pelo Círculo de Bakhtin (BAKHTIN, 2008 [1963]; VOLOCHINOV [1920] (2017), destina-se a apresentar uma análise de enunciados do gênero comentário online a respeito do vídeo “Como montar um currículo para Homeschooling”, integrante do canal de Youtube intitulado “Canal Educalar. O “Homeschooling”, conceituado neste trabalho como escolarização doméstica (PENNA, 2019; TRAVERSINNI e LOCKMANN, 2022), é um fenômeno educacional em que se reclama o direito de a família substituir, no espaço doméstico, a escolarização formal realizada comumente no espaço escolar. Tal fenômeno ganhou destaque por filiar-se aos pressupostos neoliberais e conservadores difundidos ao longo do governo Bolsonaro (2018-2022). Desse modo, a análise dos 41 comentários, presentes no vídeo, pautou-se nos percursos metodológicos presentes em Volochínov (2017) , Rodrigues (2005) e Remenche e Rohling (2016) , em que nos debruçamos a observar, primeiramente, a dimensão social do gênero comentário online para, posteriormente, analisarmos a dimensão verbal.A partir da análise dos comentários, percebeu-se a construção depreciativa do espaço escolar como um espaço prejudicial ao desenvolvimento do aluno, bem como a discursivização do homeschooling como possibilidade de proteção à diversidade social e a alunos com necessidades educacionais específicas. Mostrou-se presente, também, a vinculação aos projetos neoliberais e conservadores, como atitudes responsivas de valoração positiva a tais discursividades. Além disso, na materialidade linguageira, houve o uso de emojis depreciativos em relação à escola, o que evidencia o potencial sociocomunicativo do gênero no acento valorativo do enunciado.
PREPARATION AND PROPERTIES OF SiO2/Ag MICROBEADS USING ELECTROLESS PLATING METHOD
Authors: Zhang Congcong , Su Xiaolei , Yan Jiaqi , Liu Yi 
Published: December 2023

SiO2/Ag microbeads were synthesized using the electroless plating method by changing the addition order and the mixed method of electroless plating. Conducting composites were prepared using the prepared SiO2/Ag microbeads and unsaturated polyester resin as the fillers and substrate, respectively. The microstructure and properties of the prepared microbeads and composite were characterized with a scanning electron microscope (SEM), X-ray diffractometer (XRD), energy dispersive spectrometer (EDS), particle size analyzer, DC milliohm meter and vector network analysis tester. Results show that the SiO2/Ag microbeads prepared with the reverse dropping electroless plating method achieved the best uniformity and integrity of the silver coating on the surfaces of glass beads, and its compaction resistance reached 138.80 mΩ·cm. When the ratio of the prepared SiO2/Ag microbeads and unsaturated polyester resin was 1:2, the obtained coating composite had a resistivity of 2.79 × 10–5 Ω·cm, showing good electromagnetic shielding performance.
The accuracy of core needle biopsy in determining histological type and Scarf-Bloom-Richardson grade in invasive breast cancer
Authors: Igor Mbessoh Kengne Ulrich , Balde Salif , Ndiaye Mamadou , Mamadou Ndiaye , Sidy Ka , Ahmadou Dem 
Published: December 2023

Core needle biopsy provides information not only on the histological diagnosis but also about tumor grade and different prognosis factors for breast cancer. This study was designed to determine the accuracy of percutaneous core needle biopsy (CNB) through comparative analysis of histological diagnosis and SBR tumor grade of invasive breast cancer between CNB and surgical excision specimen (SES). This was a retrospective cross-sectional study from January 2023 to July 2023. All patients with invasive breast cancer diagnosed by CNB and confirmed by surgical excision specimen (SES) examination were included in the study. Patients who received neoadjuvant chemotherapy were excluded. One twenty-three patients were assessed. All patients were female. The median age was 45 years (37 – 54). Invasive ductal carcinoma (IDC) was the major histological type of breast cancer (95.1%) followed by invasive lobular carcinoma (ILC) (4.9%). SBR grade 2 tumors accounted for 78.1% of cases. The majority of patients presented with locally advanced stage breast cancer (65.9%) or a metastatic disease (31.7%). Concordance for histological type was substantial (k= 0.79; p&#60;0,001) whereas it was moderate (k= 0.45; p&#60;0,001) for Scarf-Bloom-Richardson grade. Histological type on CNB correlates almost perfectly with that of the surgical excisional specimen in case of breast cancer. Nevertheless, the agreement of tumor grade between CNB and SES remains moderate, with a significant underestimation and overestimation rates.
Scrape cytology – Diagnostic tool in large intestinal tumors
Authors: Patil Shashirekha , Kulkarni Nagarekha , Manickyam Shanthi , Hulikeri Shridevi S 
Published: December 2023

: Gastrointestinal tumors, mainly esophagogastric and colorectal carcinomas constitute one of the major causes of morbidity and mortality worldwide. The early detection and treatment of gastrointestinal tumors has been shown to improve patient's survival significantly. The current study is carried out to know the efficiency of scrape cytology in diagnosis of large intestinal tumors by comparing with histopathological diagnosisThirty surgically removed large intestinal tumor specimens were studied. Scrapings were taken from representative areas of each specimen, spread on slides, stained with H/E and reported. The specimens were fixed in 10% formalin, grossed and routine histopathological processing was carried out, followed by staining with H/E, examined and reported. The diagnosis obtained by scrape cytology and histopathology was correlated and analyzedTotal 30 specimens were received and majority of them were of rectal tumors(20%) and sigmund colon tumors(20%), followed by rectosigmoid region tumors (17%). Over all the scrape cytology diagnosis showed concordance for 28 cases with histopathology diagnosis having diagnostic accuracy of 93.3%.:Scrape cytology is simple, rapid and economically feasible technique and can be useful as an adjunct for frozen section in intraoperative consultation.
Uma proposta de experimento em química analítica instrumental: Determinação espectrofotométrica da constante de ionização do vermelho de alizarina em meios micelares
Authors: Mayrink Maria Isabel Cristina Batista , Sperandio Gabriel Henrique , Reis Efraim Lázaro 
Published: December 2023

Foram utilizados dois métodos para a determinação da constante de ionização condicional do indicador vermelho de alizarina S em diferentes soluções micelares a 25 oC, em força iônica constante. O primeiro método utilizado foi a derivação da curva do máximo de absorvância de cada espectro em função do pH das várias soluções. No segundo método utilizou-se a linearização de Handesson-Hasselbach, em que se utiliza também o máximo de absorvância de cada espectro em função do pH das várias soluções. Os resultados dos dois métodos mostram que a constante de ionização deste indicador é influenciada pela presença dos surfactantes neutro Triton X-100, catiônico SDS e aniônico CTAB. As técnicas potenciométrica e espectrofotométrica de uso comum em química analítica instrumental, aliadas a métodos matemáticos, mostraram-se eficientes nas determinações de parâmetros em sistemas em equilíbrio ácido-base.
Kritik atas Pemaknaan Hadis tentang Dayyūth di Media Sosial
Authors: Mukaromah Kholila 
Published: December 2023

This paper aims to criticize the meaning of the hadith which talks about dayyūth which is spread on social media in the perspective of qira’ah mubādalah. This study is included in the library research where the data is analyzed using qira’ah mubādalah analysis. The study begins with searching for some hadīths that contain the term dayyūth. The results of the study show that: first, the hadith regarding dayyūth is found in a number of hadith books: Sunan al-Nasa’i no. 2512; Musnad Ahmad no. 5904, 5839, and 5117; Musnad Abī Ya’lā no. 5430, al-Mustadrak ‘ala Ṣahihayn li al-Ḥākim no. 226; Muṣannaf Abī Razzāq no. 19.521; dan al-Mu’jam al-Awsaṭ li al-Ṭabranī with meaningful editorial and hadith quality still at the level of accepted hadith (ṣaḥīḥ). Second, in the original meaning, dayyūth is addressed to someone who has given up his family to commit an abomination. In the analysis of qira’ah mubādalah, dayyūth is not only aimed at men, but also includes women who allow their partners or family to do things that are prohibited by religion. The prohibition of becoming a dayyūth is basically a signal to always remind each other to amar ma'ruf nahi munkar in the smallest sphere of society, namely the family. Third, the results of this interpretation are therefore considered not to support the meaning of the hadith dayyūth which is spread on social media which tends to partially and literally place the blame on men as husbands or heads of families and at the same time prohibit women from appearing in public, even if it's just a photo.
Dinamika Resepsi terhadap Surah al-Fil (Analisis Teori Resepsi Hans Robert Jauss)
Authors: Rusdi Hilda Husaini 
Published: December 2023

Surah al-Fil merupakan salah satu teks ayat al-Qur’an yang menarik diteliti dari latar belakang mana pun, salah satunya dengan pendekatan teori resepsi sastra Hans Robert Jauss. Teori Jauss menekankan terhadap tanggapan pembaca atau penikmat karya sastra pada suatu teks. Wujud resepsi ini berorientasi pada pemahaman makna atau bentuk aktivitas fisik. Surah al-Fil sendiri pada masa awal Islam dibaca sebagai karya sejarah, sementara pada masa modern dituntut untuk merasionalisasi kandungan ayat. Hal ini sebagaimana al-Ghazali yang mendorong agar surah al-Fil dibaca pada rakaat kedua salat Subuh karena khasiat tertentu. Dengan menggunakan metode kualitatif dan pendekatan teori resepsi sastra Hans Robert Jauss, artikel ini menyimpulkan bahwa pembacaan surah al-Fil membentuk tanggapan dan perilaku sehingga menjadi wujud resepsi, antara lain disebabkan: pertama, kedekatan peristiwa sejarah dan turunnya teks membentuk horizon supra rasional terhadap teks. Kedua, Pengaruh global menuntut rasionalisasi sebuah teks. Duah hal ini membentuk kesalehan pribadi yang dapat menumbuhkan daya khasiat keselamatan yang direfleksikan dari kronologi kegagalan pasukan gajah dalam menyerang Mekkah.
ANÁLISE JURIMÉTRICA COMPARADA DA LEGISLAÇÃO ACERCA DE CIBERCRIME DO BRASIL E ESTADOS UNIDOS
Authors: Messias Gabriel Soares , Junior José Eronides Sousa Pequeno 
Published: December 2023

Um dos grandes desafios da conexão em massa é a regulação do ciberespaço, em especial o controle da criminalidade na internet, que provocam dados em todas as nações, no Brasil, especificamente, no ano de 2017, 62.21 milhões de pessoas foram afetadas por crimes cibernéticos, provocando um dano de 22.5 bilhões de dólares. Assim sendo, o Brasil é o segundo país em maiores danos financeiros causados por cibercrimes, ficando atrás apenas da China e logo seguido pelos Estados Unidos. O objetivo é realizar uma análise comparada entre o Brasil e Estados Unidos a fim de compreender o estado da legislação em cada um destes países. Este estudo decidiu adotar o método hierárquico (nested) juntamente de estatística descritiva com estudo de caso e análise qualitativa da legislação. As variáveis foram extraídas da Convenção de Budapeste, indicado por alguns autores como a mais avançada legislação sobre o tema. Considerando os resultados obtidos e a equivalência dos dados levantados com as variáveis, verificou-se que a legislação dos Estados Unidos possuem 90% de aderência aos parâmetros estabelecidos, enquanto o Brasil possui 70% . Assim, pode-se concluir que a legislação americana é ligeiramente mais avançada que a legislação brasileira, contudo, percebe-se que outros fatores podem envolver o fato dos danos financeiros sofridos pelos usuários brasileiros serem tão superiores.
Co-culture (Miscanthus x giganteus and Pelargonium x hortorum) phytoremediation of lead-contaminated soil, impact of this trace metal on plant physiology.
Authors: Berns Sarah , Falla-Angel Jaïro , Bonnefoy Antoine , Charrois Lucas , Laval-Gilly Philippe 
Published: December 2023

Abstract
The industrial past of most regions in Lorraine and the intensification of activities onsoils has increased the number of polluted sites. To rehabilitate these areas, several methods can be employed. The use of ornamental plants has been little studied, even if these species can be used to rehabilitate a site while improving its aesthetics. In this study, co-culture of Miscanthus x giganteus (MxG) and Pelargonium x hortorum (PxH) was used to clean up a soil mainly contaminated by Pb and trace metals. At the end of the experiment, lead concentrations were measured in the soil and plants. Furthermore, auxins, jasmonic acid, and salicylic acid were also measured to evaluate the defense mechanisms of the plants in front of pollutants. The results showed a reduction in lead concentrations following the phytoremediation process implemented and that PxH was able to extract lead from the soil. Results showed that co-culture was beneficial to the development of MxG. Concerning the molecules synthesized by the plants under stress conditions, only salicin was found in MxG roots and leaves in particular for plants grown in individual culture. According to the results obtained, it seems that co-culture can improve soil quality without their development being affected.
Dual-objective optimization of compound operations in tier-to-tier shuttle-based storage and retrieval system
Authors: Li Juntao , Hu Qixian , Lan Tian , Guo Wenwen , Wu Daqing 
Published: December 2023

Abstract
Considering the characteristics compound operations in tier-to-tier multi-shuttle system, the different sequence of warehouse tasks will lead to different working paths of the shuttles and the lifts, which will affect the operating time and energy consumption of the system. In order to reduce the operation time and energy consumption of the tier-to-tier multi-shuttle system, the dual-objective model with the shuttle operation time and energy consumption is established according to the system process compound operations scenario. According to the characteristics of the model, a hybrid particle swarm optimization-genetic simulated annealing two-level algorithm, is put forward. Then, the numerical example is calculated to verify the validity of the model and the algorithms. The experimental results show that the dual-objective model can reduce energy consumption and shuttle travel time better than the single time objective model, and can reduce time and improve work efficiency more effectively than the single energy consumption objective model.