bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics
Authors: Zhang Yu , Shi Chuanbing , Yang Yiqiong , Hu Xiuxiu , Ni Haifeng , Li Li , Cheng Zhengyuan , Huang Jing , Chen Pingsheng 
Published: November 2023

AbstractRenal interstitial fibrosis (RIF) is a key feature of progressive chronic kidney disease (CKD), characterized by tubular epithelial cell (TEC) hypoxia and peritubular capillary (PTC) rarefaction. However, the mechanisms underlying these processes remain poorly understood. To address this knowledge gap, we conducted a comparative transcriptome analysis of hypoxic and normoxic HK-2 cells, identifying 572 differentially expressed genes (DEGs). Subsequent Gene Ontology (GO), protein‒protein interaction (PPI) network, and hub gene analyses revealed significant enrichment of DEGs in the HIF-1 signaling pathway based on KEGG enrichment analysis. To further explore TEC modulation under hypoxic conditions, we performed chromatin immunoprecipitation (ChIP) sequencing targeting HIF-1α, identifying 2915 genes potentially regulated by HIF-1α. By comparing RNA sequencing and ChIP sequencing data, we identified 43 overlapping DEGs. By performing GO analysis and peak annotation with IGV, we identified two candidate molecules, VEGFA and BTG1, that are associated with angiogenesis and whose gene sequences were reliably bound by HIF-1α. Our study elucidates the molecular mechanisms underlying RIF, providing valuable insights for potential therapeutic interventions.
Growth and fatty acid composition of pikeperch (Sander lucioperca L., 1758) larvae under altered feeding protocol including the copepod Apocyclops panamensis (Marsh, 1913)
Authors: Ballesteros-Redondo Laura , Palm Harry W. , Bährs Hanno , Rapp Tobias , Stueeken Marcus , Wacker Alexander , Bischoff Adrian A. 
Published: November 2023

AbstractAlternative live feeds for small and sensitive fish early life stages such as pikeperch (Sander lucioperca L., 1758) can improve the larval quantity, quality and performance in aquaculture. Therefore, this study evaluated the cyclopoid copepod Apocyclops panamensis (Marsh, 1913) as live feed for pikeperch larviculture from day 11 post hatch (dph) in two independent experiments. In both experiments, pikeperch larvae had the highest specific growth rate (SGR) when they fed on Brachionus plicatilis until dph 11 and A. panamensis until dph 16–18. SGR was related to a decrease in total fatty acids (FAs), saturated FAs and monounsaturated FAs in pikeperch larvae, indicating their use as energy for growth. Within the polyunsaturated FAs, docosahexaenoic acid (DHA) increased in larvae fed with A. panamensis and coincided with the highest SGR suggesting that DHA is accumulated in larvae as structural FA. Our study demonstrated a suitable pikeperch larval fatty acid composition for growth after feeding A. panamensis compared with Artemia sp. from dph 11 until dph 16 and previously fed with B. plicatilis. Moreover, it highlighted the importance of the dietary PUFAs in pikeperch rearing, specifically of linoleic acid (LA) from dph 4 until dph 11 and of DHA from dph 11 onwards.
Toward MR protocol-agnostic, unbiased brain age predicted from clinical-grade MRIs
Authors: Valdes-Hernandez Pedro A. , Laffitte Nodarse Chavier , Peraza Julio A. , Cole James H. , Cruz-Almeida Yenisel 
Published: November 2023

AbstractThe difference between the estimated brain age and the chronological age (‘brain-PAD’) could become a clinical biomarker. However, most brain age models were developed for research-grade high-resolution T1-weighted MRIs, limiting their applicability to clinical-grade MRIs from various protocols. We adopted a dual-transfer learning strategy to develop a model agnostic to modality, resolution, or slice orientation. We retrained a convolutional neural network (CNN) using 6281 clinical MRIs from 1559 patients, among 7 modalities and 8 scanner models. The CNN was trained to estimate brain age from synthetic research-grade magnetization-prepared rapid gradient-echo MRIs (MPRAGEs) generated by a ‘super-resolution’ method. The model failed with T2-weighted Gradient-Echo MRIs. The mean absolute error (MAE) was 5.86–8.59 years across the other modalities, still higher than for research-grade MRIs, but comparable between actual and synthetic MPRAGEs for some modalities. We modeled the “regression bias” in brain age, for its correction is crucial for providing unbiased summary statistics of brain age or for personalized brain age-based biomarkers. The bias model was generalizable as its correction eliminated any correlation between brain-PAD and chronological age in new samples. Brain-PAD was reliable across modalities. We demonstrate the feasibility of brain age predictions from arbitrary clinical-grade MRIs, thereby contributing to personalized medicine.
Dexamethasone with aggressive warming facilitates pain reduction, reduced blood loss, and quicker recovery after total hip arthroplasty
Authors: Li Fulin , Huang Xiao , Liu Wenhui , Huang Wenwen , Cheng Jinwen , Yin Dong 
Published: November 2023

AbstractThis study aimed to evaluate the optimal frequency of dexamethasone (DEX) administration and the efficacy of DEX with aggressive warming in total hip arthroplasty (THA), which remains unclear. A total of 150 patients were treated with DEX (10 mg) once before and once or twice after surgery with or without intraoperative aggressive warming. On postoperative day 3, the dynamic visual analogue scale scores and C-reactive protein (CRP) and interleukin-6 (IL-6) levels in participants administered with DEX twice after surgery were significantly lower than those who did not receive the second dose. The range of motion (ROM), postoperative fatigue based on Identity-Consequence-Fatigue Scale, average temperature at different stages, intraoperative blood loss, and postoperative drainage volume in patients who were subjected to warming were significantly higher than those who were not. The degree of satisfaction was also higher in the patients who received both second dose and warming than those who received neither. No differences in complications were observed based on the treatments. An additional dose of DEX at 48 h post-surgery has short-term advantages in terms of analgesia, anti-inflammatory effects, and accelerated rehabilitation after THA. DEX combined with aggressive warming further optimises short-term ROM and fatigue and improves the degree of satisfaction.Clinical trial was registered in the International Clinical Trial Registry, and the date of registration is 2/12/2020 (ChiCTR2000040560).
Spring biomass burning in Indochina enhances summer Yangtze River Valley rainfall through land–atmosphere interactions
Authors: Wei Jiangfeng , Mao Qianqian , Shan Yufeng , Jin Qinjian , Yang Yang , Chen Haishan 
Published: November 2023

AbstractIndochina is one of the regions with the most severe biomass burning (BB) in the world, which produces enormous amounts of atmospheric aerosols, mostly in spring. Moreover, the spring soil moisture anomalies in Indochina have been found to have a significant influence on the summer rainfall in the Yangtze River Valley (YRV). It is still partially unclear how spring BB in Indochina influences the local and regional climate and land–atmosphere interactions. Here, we use regional model experiments and observational data to show that the radiative effect of BB aerosols in Indochina stabilizes the atmosphere and reduces local precipitation and soil moisture. The dry soil in Indochina persists from spring to summer, which warms the land surface and the atmosphere. As a result, the western Pacific subtropical high (WPSH) is stronger and extends westward from spring to summer. This leads to stronger moisture transport to and more precipitation in the YRV (increases of approximately 5% in July and 10% in August). Thus, the effect of BB aerosols on the YRV rainfall is similar to that of the Indochina dry soil anomaly. Additionally, the increase in the YRV summer rainfall caused by the Indochina spring dry soil anomaly is almost doubled when there are BB aerosols compared to that without BB aerosols, suggesting the importance of BB aerosols in regulating the regional land–atmosphere interactions.
Deep learning-based image analysis identifies a DAT-negative subpopulation of dopaminergic neurons in the lateral Substantia nigra
Authors: Burkert Nicole , Roy Shoumik , Häusler Max , Wuttke Dominik , Müller Sonja , Wiemer Johanna , Hollmann Helene , Oldrati Marvin , Ramirez-Franco Jorge , Benkert Julia , Fauler Michael , Duda Johanna , Goaillard Jean-Marc , Pötschke Christina , Münchmeyer Moritz , Parlato Rosanna , Liss Birgit 
Published: November 2023

AbstractHere we present a deep learning-based image analysis platform (DLAP), tailored to autonomously quantify cell numbers, and fluorescence signals within cellular compartments, derived from RNAscope or immunohistochemistry. We utilised DLAP to analyse subtypes of tyrosine hydroxylase (TH)-positive dopaminergic midbrain neurons in mouse and human brain-sections. These neurons modulate complex behaviour, and are differentially affected in Parkinson’s and other diseases. DLAP allows the analysis of large cell numbers, and facilitates the identification of small cellular subpopulations. Using DLAP, we identified a small subpopulation of TH-positive neurons (~5%), mainly located in the very lateral Substantia nigra (SN), that was immunofluorescence-negative for the plasmalemmal dopamine transporter (DAT), with ~40% smaller cell bodies. These neurons were negative for aldehyde dehydrogenase 1A1, with a lower co-expression rate for dopamine-D2-autoreceptors, but a ~7-fold higher likelihood of calbindin-d28k co-expression (~70%). These results have important implications, as DAT is crucial for dopamine signalling, and is commonly used as a marker for dopaminergic SN neurons.
Uncoupled evolution of the Polycomb system and deep origin of non-canonical PRC1
Authors: de Potter Bastiaan , Raas Maximilian W. D. , Seidl Michael F. , Verrijzer C. Peter , Snel Berend 
Published: November 2023

AbstractPolycomb group proteins, as part of the Polycomb repressive complexes, are essential in gene repression through chromatin compaction by canonical PRC1, mono-ubiquitylation of histone H2A by non-canonical PRC1 and tri-methylation of histone H3K27 by PRC2. Despite prevalent models emphasizing tight functional coupling between PRC1 and PRC2, it remains unclear whether this paradigm indeed reflects the evolution and functioning of these complexes. Here, we conduct a comprehensive analysis of the presence or absence of cPRC1, nPRC1 and PRC2 across the entire eukaryotic tree of life, and find that both complexes were present in the Last Eukaryotic Common Ancestor (LECA). Strikingly, ~42% of organisms contain only PRC1 or PRC2, showing that their evolution since LECA is largely uncoupled. The identification of ncPRC1-defining subunits in unicellular relatives of animals and fungi suggests ncPRC1 originated before cPRC1, and we propose a scenario for the evolution of cPRC1 from ncPRC1. Together, our results suggest that crosstalk between these complexes is a secondary development in evolution.
Social Media für Heilpraktiker
Authors: Pietruck Vivien 
Published: November 2023

SummaryWährend Facebook als Allround- und Gruppenplattform vor allem (auch ältere) Erwachsene anspricht, richten sich bild- und videobetonte Netzwerke wie Instagram, Pinterest und TikTok eher an ein jüngeres Publikum, wohingegen Xing und LinkedIn die berufliche Vernetzung in den Fokus stellen. Wer die jeweiligen Algorithmen nutzt, mehrere Plattformen synchron bestückt und dies durch Social-Media-Advertising ergänzt, kann seine Reichweite deutlich erhöhen. Der Social-Media-Auftritt einer Heilpraktikerpraxis erfordert täglichen Arbeitseinsatz sowie gründliche Überlegungen und Vorbereitungen hinsichtlich Rechtskonformität, Zielgruppe, geeigneter Plattform, Aufwand und Nutzen.
Case Series of Reproductive Outcomes after Surgical Correction of Obstructed Hemivagina in OHVIRA
Authors: Bunnell Megan E. , Cipres Danielle T. , Laufer Marc R. 
Published: November 2023

AbstractObstructed hemivagina and ipsilateral renal anomaly (OHVIRA) is a rare congenital developmental syndrome manifested by uterine duplication, lower genital tract obstruction, and unilateral renal anomaly. Literature on reproductive outcomes in this patient population is limited. The aim of this study is to describe obstetric outcomes after surgical correction of obstructed hemivagina in a longitudinal cohort of patients with a diagnosis of OHVIRA. All cases of OHVIRA presenting to a single tertiary care children's hospital from 1990 to 2021 were retrospectively reviewed. Three cases demonstrating a variety of clinically important reproductive outcomes are described in detail including risks such as retained products, endometritis, preterm labor, and malpresentation. Understanding the reproductive outcomes associated with this diagnosis is important for practitioners seeking to counsel and care for patients with this diagnosis. This case series demonstrates a wide array of potential gynecologic and obstetric risks, though ultimately with successful term and near-term pregnancies.
Reverse Wafer Procedure
Authors: Ozcelik Ismail Bulent , Cavit Ali , Yuceturk Aydin 
Published: November 2023

Abstract
Purpose This study aimed to present the results of an alternative technique for treating ulnar impaction syndrome, the “reverse wafer procedure,” and assess the mid-term clinical outcomes of patients treated with this technique.
Methods A retrospective evaluation was conducted on 14 patients who underwent the reverse wafer procedure for ulnar impaction syndrome between 2013 and 2020. The inclusion criteria for the study were persistent ulnar-sided wrist pain, even after a minimum of 3 months of nonoperative treatment, the presence of a chondral lesion on the lunate, and an intact triangular fibrocartilage complex (TFCC) based on arthroscopic evaluation. Postoperative clinical and subjective functional outcomes were assessed using measures such as wrist range of motion (ROM), grip strength, the Turkish version of the quick disabilities of the arm, shoulder, and hand questionnaire (Quick DASH), and pain levels measured on a visual analog scale (VAS) and were compared with preoperative values. Patient satisfaction was also evaluated postoperatively.
Results The mean follow-up period was 42.2 months (range: 24–68 months). Postoperatively, none of the patients experienced any restriction in ROM. Grip measurements significantly increased after the surgery (p = 0.003). Preoperatively, the patients had a grip strength of 41.14 kg (range 28–48 kg), which improved to 44 kg (range 30–52 kg) postoperatively. Postoperative VAS values and QDASH scores significantly decreased compared with the preoperative values (p < 0.001, p = 0.001). The mean VAS score decreased from 6.1 (range 4–8) preoperatively to 0.9 (range 0–5) postoperatively. The mean Quick DASH score decreased from 49.44 (range 25–68.3) preoperatively to 10.13 (range 3.3–36) postoperatively. When asked about their satisfaction with the operation, 13 out of 14 patients reported being highly satisfied with the results.
Conclusions The reverse wafer procedure presents an alternative treatment option for ulnar impaction syndrome in cases where the TFCC is intact. The mid-term results of this described technique are promising. However, further comparative studies with longer follow-ups are necessary to support these findings.
Level of Evidence IV Therapeutic.
A framework for developing team science expertise using a reflective-reflexive design method (R2DM)
Authors: Lotrecchiano Gaetano R. , Bennett L. Michelle , Vovides Yianna 
Published: November 2023

AbstractEffective integration and implementation of knowledge in research are dependent on team science expertise grounded in collaboration principles and techniques that advance individual and group scientific agendas. The Science of Team Science (SciTS) provides evidence-based research and best practices that strive to develop scientists’ collaborative skills so that they can work across disciplinary boundaries while developing strong and diverse teaming relationships. Identifying the motivations of those involved in collaborative teaming can contribute to maximizing team effectiveness and applying the knowledge emerging from understanding these to shape teams’ adaptation of a shared mutual learning mindset as a core tenet of scientific teamwork. In addition, surfacing motivations has the potential of helping team members examine their own needs in relation to their scientific and career goals. In this paper we draw from the domains of the Motivation Assessment for Team, Readiness, Integration, and Collaboration (MATRICx) framework, Maslow’s Hierarchy of Needs motivational theory, and The Team Effectiveness Model for Science (TEMS) to develop a Reflective-Reflexive Design Method (R2DM) that focuses on the development of intra-personal attributes within the context of a team. Approaching expertise development from this design method invites individual reflection in the context of group reflexivity to serve as the cornerstone of deep team science expertise. We used a design thinking approach to identify a framework that merges individual reflection with group reflexivity. The core questions we asked are: (i) What constitutes expertise to succeed in science teams? and (ii) How might we approach the design of learning engagements that enable the development of the needed expertise?
Final report, ongoing key comparison BIPM.QM-K1, ozone at ambient level, comparison with ISCIII, May 2023
Authors: Viallon Joële , Idrees Faraz , Moussay Philippe , Wielgosz Robert , Nunez-Corcuera Beatriz , Santos-Alves Saúl Garcia Dos 
Published: November 2023

Main text
As part of the ongoing key comparison BIPM.QM-K1, a comparison has been performed between the ozone national standard of the Instituto de Salud Carlos III (ISCIII) and the common reference standard of the key comparison, maintained by the Bureau International des Poids et Mesures (BIPM). The instruments have been compared over a nominal ozone amount-of-substance fraction range of 0 nmol mol−1 to 500 nmol mol−1.
To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database https://www.bipm.org/kcdb/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Simulation study on influence of oxygen concentrations on atmospheric-pressure streamer in oxygen-rich nitrogen–oxygen mixture
Authors: Wei Zhenyu , Komuro Atsushi , Ono Ryo 
Published: November 2023

Abstract
This study aims to investigate the behaviour of positive streamer discharge in various

N
2

/

O
2

mixtures with different

O
2

concentrations via two-dimensional simulations. The proposed model considers the effects of photoionisation caused by the photons generated by excited oxygen atoms

O
I

, which enables the simulation of streamers in oxygen rich conditions. The simulation results indicate that the propagation velocity and diameter of the streamer increase with the oxygen concentration, while the emission intensity decreases. The result conforms to the experimental results of our previous work. Moreover, the simulation results suggest that changes in the ionisation rate caused by variations in the oxygen concentration affect the streamer propagation, while the impact of photoionisation is limited.
Investigation on the effect of annealing period on the structural and optical properties of ZnO thin films prepared using a polymer precursor
Authors: Mathew Jijoy. P. 
Published: November 2023

Abstract
Numerous synthesis strategies including sputtering,molecular beam epitaxy,chemical vapour deposition, pulsed laser deposition etc., have been reported to grow high quality ZnO thin films[1]. However among these mounted synthesis strategies, simple and price powerful path to develop ZnO thin films by way of usage of cheaper, non poisonous and environmentally benign precursors are nevertheless the important issues. This work advise a simple and novel technique to the fabrication of ZnO thin films with the aid of poly vinyl alcohol (PVA) as precursor. This article also investigate the impact of annealing period on the structural and optical properties of grown films by analyzing XRD, UV absorption and photo luminescence spectra. The XRD pattern reveals poly crystalline hexagonal wurtzite structure. The particle size and stress values varies with changes in annealing period. The values of UV absorption spectra are utilized to plot Tauc plot to get the band gap of the films. The band gap shows significant decrease from 3.35ev to 3.10ev with increase in annealing period. The films shows good transmittance >75% and transmittance increase as the annealing parameter increases. The PL spectra have peaks in UV and visible regions,. the mechanism behind these emission peaks are also discussed.
Dispersion stability analysis of copper sulphide nanoparticles in different medium
Authors: Mukherjee Bodhoday , Okram Gunadhor Singh 
Published: November 2023

Abstract
Nanoparticles (NPs) of covellite CuS have been synthesised using a modified polyol method at 160ºC - 165ºC without adding any extra surfactant. These NPs were charecterized by X-ray diffraction (XRD), Raman spectroscopy, Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive Analysis of X-ray (EDAX), Zeta potential and Dynamic Light Scattering (DLS) techniques. XRD data and three Raman modes confirm P63/mmc space group and hexagonal crystal structure of CuS NPs. EDAX data of Cu:S was slight differed from nominal 1:1 ratio due to extra S taken in synthesis. FESEM images show sheet-like NPs. The zeta potential (ξ) data analysis shows that the stability of the NPs is dependent on the dispersion medium, with high stability observed above pH 8.93 in an acidic medium. At pH 10.95, a highest ξ value of -37.64 mV is recorded in de-ionized water (DIW), while the nanoparticles are found to be unstable in ethanol and propanol as dispersion mediums. According to the hydrodynamic diameter (HD) data, the particles tend to agglomerate more quickly in ethanol and propanol compared to DIW. These findings are expected to be useful in various applications such as pigments in paints, batteries, pharmaceuticals, ceramics, and waste water treatment.
Radiation-temperature dependence of water at microwaves
Authors: Gavrilin S N 
Published: November 2023

Abstract
Pure water radiation-temperature dependence at wavelength 8 mm in the temperature range of 30-50 °C has been experimentally investigated. Measurements were made with the microwave radiometer with central frequency 37.5 GHz (wavelength 8 mm). The absolute method of measuring the intensities of distributed radiation was used. A horn with a 30x30 mm opening was used as an antenna. Calibration of the intensity of the received radiation was carried out by its own radiation of a black body at different temperatures. The equipment had a sensitivity (fluctuation threshold) 0.2 K at a time constant τ = 1 sec. An increment of the radio-brightness contrast is about 3 K in this temperature range. The water radio-emission depends non-linearly on the kinetic temperature. It was measured, that effective temperature of the skin-depth layer increases from 0.28 up to 0.4 °C per degree of water heating. It was shown, that the radiation temperature of the water surface for the 8 mm waves and temperature range 30-50 °C is determined not by the bulk kinetic water temperature, but by the effective temperature of the skin-depth layer formed under the influence of heat exchange with the atmosphere.