bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phase separation of BuGZ regulates gut regeneration and aging through interaction with m6A regulators
Authors: Zhang Qiaoqiao , Deng Kai , Liu Mengyou , Yang Shengye , Xu Wei , Feng Tong , Jie Minwen , Liu Zhiming , Sheng Xiao , Chen Haiyang , Jiang Hao 
Published: October 2023

AbstractExploring the role of phase separation in intracellular compartment formation is an active area of research. However, the associations of phase separation with intestinal stem cell (ISC)-dependent regeneration and aging remain unclear. Here, we demonstrate that BuGZ, a coacervating mitotic effector, shows age- and injury-associated condensation in Drosophila ISC nuclei during interphase. BuGZ condensation promotes ISC proliferation, affecting Drosophila gut repair and longevity. Moreover, m6A reader YT521-B acts as the transcriptional and functional downstream of BuGZ. The binding of YT521-B promotor or m6A writer Ime4/ Mettl14 to BuGZ controls its coacervation, indicating that the promotor may accelerate the phase transition of its binding transcription factor. Hence, we propose that phase separation and m6A regulators may be critical for ameliorating ISC-dependent gut regeneration and aging and requires further study.
Acylation of glycerolipids in mycobacteria
Authors: Angala Shiva Kumar , Carreras-Gonzalez Ana , Huc-Claustre Emilie , Anso Itxaso , Kaur Devinder , Jones Victoria , Palčeková Zuzana , Belardinelli Juan M. , de Sousa-d’Auria Célia , Shi Libin , Slama Nawel , Houssin Christine , Quémard Annaïk , McNeil Michael , Guerin Marcelo E. , Jackson Mary 
Published: October 2023

AbstractWe report on the existence of two phosphatidic acid biosynthetic pathways in mycobacteria, a classical one wherein the acylation of the sn-1 position of glycerol-3-phosphate (G3P) precedes that of sn-2 and another wherein acylations proceed in the reverse order. Two unique acyltransferases, PlsM and PlsB2, participate in both pathways and hold the key to the unusual positional distribution of acyl chains typifying mycobacterial glycerolipids wherein unsaturated substituents principally esterify position sn-1 and palmitoyl principally occupies position sn-2. While PlsM selectively transfers a palmitoyl chain to the sn-2 position of G3P and sn-1-lysophosphatidic acid (LPA), PlsB2 preferentially transfers a stearoyl or oleoyl chain to the sn-1 position of G3P and an oleyl chain to sn-2-LPA. PlsM is the first example of an sn-2 G3P acyltransferase outside the plant kingdom and PlsB2 the first example of a 2-acyl-G3P acyltransferase. Both enzymes are unique in their ability to catalyze acyl transfer to both G3P and LPA.
MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism
Authors: Dluzewska Julia , Dziegielewski Wojciech , Szymanska-Lejman Maja , Gazecka Monika , Henderson Ian R. , Higgins James D. , Ziolkowski Piotr A. 
Published: October 2023

AbstractMeiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can stimulate interfering crossovers in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the two pathways, we combined Arabidopsis mutants that elevate non-interfering crossovers with msh2 mutants. We demonstrate that MSH2 blocks non-interfering crossovers at polymorphic loci, which is the opposite effect to interfering crossovers. We also observe MSH2-independent crossover inhibition at highly polymorphic sites. We measure recombination along the chromosome arms in lines differing in patterns of heterozygosity and observe a MSH2-dependent crossover increase at the boundaries between heterozygous and homozygous regions. Here, we show that MSH2 is a master regulator of meiotic DSB repair in Arabidopsis, with antagonistic effects on interfering and non-interfering crossovers, which shapes the crossover landscape in relation to interhomolog polymorphism.
A transcriptional activator effector of Ustilago maydis regulates hyperplasia in maize during pathogen-induced tumor formation
Authors: Zuo Weiliang , Depotter Jasper R. L. , Stolze Sara Christina , Nakagami Hirofumi , Doehlemann Gunther 
Published: October 2023

AbstractUstilago maydis causes common smut in maize, which is characterized by tumor formation in aerial parts of maize. Tumors result from the de novo cell division of highly developed bundle sheath and subsequent cell enlargement. However, the molecular mechanisms underlying tumorigenesis are still largely unknown. Here, we characterize the U. maydis effector Sts2 (Small tumor on seedlings 2), which promotes the division of hyperplasia tumor cells. Upon infection, Sts2 is translocated into the maize cell nucleus, where it acts as a transcriptional activator, and the transactivation activity is crucial for its virulence function. Sts2 interacts with ZmNECAP1, a yet undescribed plant transcriptional activator, and it activates the expression of several leaf developmental regulators to potentiate tumor formation. On the contrary, fusion of a suppressive SRDX-motif to Sts2 causes dominant negative inhibition of tumor formation, underpinning the central role of Sts2 for tumorigenesis. Our results not only disclose the virulence mechanism of a tumorigenic effector, but also reveal the essential role of leaf developmental regulators in pathogen-induced tumor formation.
Atmospheric-moisture-induced polyacrylate hydrogels for hybrid passive cooling
Authors: Galib Roisul Hasan , Tian Yanpei , Lei Yue , Dang Saichao , Li Xiaole , Yudhanto Arief , Lubineau Gilles , Gan Qiaoqiang 
Published: October 2023

AbstractHeat stress is being exacerbated by global warming, jeopardizing human and social sustainability. As a result, reliable and energy-efficient cooling methods are highly sought-after. Here, we report a polyacrylate film fabricated by self-moisture-absorbing hygroscopic hydrogel for efficient hybrid passive cooling. Using one of the lowest-cost industrial materials (e.g., sodium polyacrylate), we demonstrate radiative cooling by reducing solar heating with high solar reflectance (0.93) while maximizing thermal emission with high mid-infrared emittance (0.99). Importantly, the manufacturing process utilizes only atmospheric moisture and requires no additional chemicals or energy consumption, making it a completely green process. Under sunlight illumination of 800 W m−2, the surface temperature of the film was reduced by 5 °C under a partly cloudy sky observed at Buffalo, NY. Combined with its hygroscopic feature, this film can simultaneously introduce evaporative cooling that is independent of access to the clear sky. The hybrid passive cooling approach is projected to decrease global carbon emissions by 118.4 billion kg/year compared to current air-conditioning facilities powered by electricity. Given its low-cost raw materials and excellent molding feature, the film can be manufactured through simple and cost-effective roll-to-roll processes, making it suitable for future building construction and personal thermal management needs.
Local decorin delivery via hyaluronic acid microrods improves cardiac performance, ventricular remodeling after myocardial infarction
Authors: Mohindra Priya , Zhong Justin X. , Fang Qizhi , Cuylear Darnell L. , Huynh Cindy , Qiu Huiliang , Gao Dongwei , Kharbikar Bhushan N. , Huang Xiao , Springer Matthew L. , Lee Randall J. , Desai Tejal A. 
Published: October 2023

AbstractHeart failure (HF) remains a global public health burden and often results following myocardial infarction (MI). Following injury, cardiac fibrosis forms in the myocardium which greatly hinders cellular function, survival, and recruitment, thus severely limits tissue regeneration. Here, we leverage biophysical microstructural cues made of hyaluronic acid (HA) loaded with the anti-fibrotic proteoglycan decorin to more robustly attenuate cardiac fibrosis after acute myocardial injury. Microrods showed decorin incorporation throughout the entirety of the hydrogel structures and exhibited first-order release kinetics in vitro. Intramyocardial injections of saline (n = 5), microrods (n = 7), decorin microrods (n = 10), and free decorin (n = 4) were performed in male rat models of ischemia-reperfusion MI to evaluate therapeutic effects on cardiac remodeling and function. Echocardiographic analysis demonstrated that rats treated with decorin microrods (5.21% ± 4.29%) exhibited significantly increased change in ejection fraction (EF) at 8 weeks post-MI compared to rats treated with saline (−4.18% ± 2.78%, p < 0.001) and free decorin (−3.42% ± 1.86%, p < 0.01). Trends in reduced end diastolic volume were also identified in decorin microrod-treated groups compared to those treated with saline, microrods, and free decorin, indicating favorable ventricular remodeling. Quantitative analysis of histology and immunofluorescence staining showed that treatment with decorin microrods reduced cardiac fibrosis (p < 0.05) and cardiomyocyte hypertrophy (p < 0.05) at 8 weeks post-MI compared to saline control. Together, this work aims to contribute important knowledge to guide rationally designed biomaterial development that may be used to successfully treat cardiovascular diseases.
Droplet slipperiness despite surface heterogeneity at molecular scale
Authors: Lepikko Sakari , Jaques Ygor Morais , Junaid Muhammad , Backholm Matilda , Lahtinen Jouko , Julin Jaakko , Jokinen Ville , Sajavaara Timo , Sammalkorpi Maria , Foster Adam S. , Ras Robin H. A. 
Published: October 2023

AbstractFriction determines whether liquid droplets slide off a solid surface or stick to it. Surface heterogeneity is generally acknowledged as the major cause of increased contact angle hysteresis and contact line friction of droplets. Here we challenge this long-standing premise for chemical heterogeneity at the molecular length scale. By tuning the coverage of self-assembled monolayers (SAMs), water contact angles change gradually from about 10° to 110° yet contact angle hysteresis and contact line friction are low for the low-coverage hydrophilic SAMs as well as high-coverage hydrophobic SAMs. Their slipperiness is not expected based on the substantial chemical heterogeneity of the SAMs featuring uncoated areas of the substrate well beyond the size of a water molecule as probed by metal reactants. According to molecular dynamics simulations, the low friction of both low- and high-coverage SAMs originates from the mobility of interfacial water molecules. These findings reveal a yet unknown and counterintuitive mechanism for slipperiness, opening new avenues for enhancing the mobility of droplets.
An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity
Authors: Park Sang-Youl , Qiu Jingde , Wei Shuang , Peterson Francis C. , Beltrán Jesús , Medina-Cucurella Angélica V. , Vaidya Aditya S. , Xing Zenan , Volkman Brian F. , Nusinow Dmitri A. , Whitehead Timothy A. , Wheeldon Ian , Cutler Sean R. 
Published: October 2023

AbstractPlants sense abscisic acid (ABA) using chemical-induced dimerization (CID) modules, including the receptor PYR1 and HAB1, a phosphatase inhibited by ligand-activated PYR1. This system is unique because of the relative ease with which ligand recognition can be reprogrammed. To expand the PYR1 system, we designed an orthogonal ‘*’ module, which harbors a dimer interface salt bridge; X-ray crystallographic, biochemical and in vivo analyses confirm its orthogonality. We used this module to create PYR1*MANDI/HAB1* and PYR1*AZIN/HAB1*, which possess nanomolar sensitivities to their activating ligands mandipropamid and azinphos-ethyl. Experiments in Arabidopsis thaliana and Saccharomyces cerevisiae demonstrate the sensitive detection of banned organophosphate contaminants using living biosensors and the construction of multi-input/output genetic circuits. Our new modules enable ligand-programmable multi-channel CID systems for plant and eukaryotic synthetic biology that can empower new plant-based and microbe-based sensing modalities.
1-km resolution rebound surfaces and paleotopography of glaciated North America since the Last Glacial Maximum
Authors: Godbout Pierre-Marc , Brouard Etienne , Roy Martin 
Published: October 2023

AbstractWe present a series of 1-km spatial resolution rebound (isobase) surfaces based on publicly distributed predictions obtained from the glacio-isostatic adjustment models known as ICE-5G (VM2 L90), ICE-6G_C (VM5a) and ICE-7G_NA (VM7). Our objective is to provide readily accessible tools for a broad range of geological and paleoenvironmental studies, and to facilitate direct comparison between models’ predictions and field-based observations. Rebound surfaces were interpolated at the scale of North American ice sheets (35.5°-89.5°N; 45°-165°W) and for each time increment of the models (1,000-500 yrs, between 26,000-21,000 yrs BP and present-day). The assessment of the interpolations indicates that the rebound surfaces have an overall vertical accuracy of ∼0.4 m compared to original ICE-xG outputs. These rebound surfaces were combined with the GEBCO 2021 present-day elevation grid to reconstruct the paleotopography for each time increment of the models and are all presented as raster files that can be easily integrated into geographical information systems. The resulting datasets therefore provide a unique support for geological, paleoenvironmental and archeological studies.
Adequacy of in-mission training to treat tibial shaft fractures in mars analogue testing
Authors: Manon Julie , Saint-Guillain Michael , Pletser Vladimir , Buckland Daniel Miller , Vico Laurence , Dobney William , Baatout Sarah , Wain Cyril , Jacobs Jean , Comein Audrey , Drouet Sirga , Meert Julien , Casla Ignacio Sanchez , Chamart Cheyenne , Vanderdonckt Jean , Cartiaux Olivier , Cornu Olivier 
Published: October 2023

AbstractLong bone fractures are a concern in long-duration exploration missions (LDEM) where crew autonomy will exceed the current Low Earth Orbit paradigm. Current crew selection assumptions require extensive complete training and competency testing prior to flight for off-nominal situations. Analogue astronauts (n = 6) can be quickly trained to address a single fracture pattern and then competently perform the repair procedure. An easy-to-use external fixation (EZExFix) was employed to repair artificial tibial shaft fractures during an inhabited mission at the Mars Desert Research Station (Utah, USA). Bone repair safety zones were respected (23/24), participants achieved 79.2% repair success, and median completion time was 50.04 min. Just-in-time training in-mission was sufficient to become autonomous without pre-mission medical/surgical/mechanical education, regardless of learning conditions (p > 0.05). Similar techniques could be used in LDEM to increase astronauts’ autonomy in traumatic injury treatment and lower skill competency requirements used in crew selection.
Screen time, impulsivity, neuropsychological functions and their relationship to growth in adolescent attention-deficit/hyperactivity disorder symptoms
Authors: Wallace Jasmina , Boers Elroy , Ouellet Julien , Afzali Mohammad H. , Conrod Patricia 
Published: October 2023

AbstractPrevious longitudinal studies found significant associations between screen time and increase in attention-deficit/hyperactivity disorder (ADHD) symptoms, but the mechanisms mediating this association remain understudied. Thus, we used data from a 5-year population-based longitudinal cohort of nearly 4000 Canadian high school students, modeled using multivariate multilevel mediation, to investigate the association of screen time (i.e., social media, television, video games, computer use) with ADHD symptoms via different potential behavioral and neuropsychological mediators (i.e. impulsivity, response inhibition, working memory). We studied direct and indirect between-person, concurrent within-person, and lagged-within-person effects of screens on ADHD symptoms. Results showed that increases in screen time in a given year were associated with an exacerbation of ADHD symptoms within that same year (within-person association), over and above potential common vulnerability (between-person association). Impulsivity proved to be the most robust mediator in the association of screen time with ADHD symptoms at both between and within-person levels. Only social media use displayed a significant lagged-within-person association with ADHD symptoms mediated by impulsivity, indicating an enduring influence on behavior, which was further shown to be mediated by chained changes in response inhibition on a Go/No-Go task. These findings provide clinical implications of screen time and should be an important focus in the management and prevention of ADHD symptoms among adolescents.
Predicting in-hospital cardiac arrest outcomes: CASPRI and GO-FAR scores
Authors: Jung Jonghee , Ryu Ji Ho , Shon Seungwoo , Min Munki , Hyun Tae Gyu , Chun Mose , Lee Daesup , Lee Minjee 
Published: October 2023

AbstractIt is important to predict the neurological prognoses of in-hospital cardiac arrest (IHCA) patients immediately after recovery of spontaneous circulation (ROSC) to make further critical management. The aim of this study was to confirm the usefulness of the Cardiac Arrest Survival Post-Resuscitation In-hospital (CASPRI) and Good Outcome Following Attempted Resuscitation (GO-FAR) scores for predicting the IHCA immediately after the ROSC. This is a retrospective analysis of patient data from a tertiary general hospital located in South Korea. A total of 488 adult patients who had IHCA and achieved sustained ROSC from September 2016 to August 2021 were analyzed to compare effectiveness of the CASPRI and GO-FAR scores related to neurologic prognosis. The primary outcome was Cerebral Performance Category (CPC) score at discharge, defined as a CPC score of 1 or 2. The secondary outcomes were survival-to-discharge and normal neurological status or minimal neurological damage at discharge. Of the 488 included patients, 85 (20.8%) were discharged with good prognoses (CPC score of 1 or 2). The area under the receiver operating characteristic curve of CASPRI score for the prediction of a good neurological outcome was 0.75 (95% CI 0.69–0.81), whereas that of GO-FAR score was 0.67 (95% CI 0.60–0.73). The results of this study show that these scoring systems can be used for timely and satisfactory prediction of the neurological prognoses of IHCA patients after ROSC.
Predicting salivary cortisol and sexual behavior stigma among MSM in the American Men’s Internet Survey 2019
Authors: Dibble Kate E. , Murray Sarah M. , Baral Stefan D. , Zlotorzynska Maria , Wiginton John Mark , Stephenson Rob , Edwards O. Winslow , Lyons Carrie , Rainey Jacob C. , Xue Qian-Li , Sanchez Travis H. 
Published: October 2023

AbstractPhysiological stress levels in response to sexual behavior stigma among men who have sex with men (MSM) in the United States (US) are understudied. The current study aims to explore the relationship between sexual behavior stigma and salivary cortisol both overall and stratified by race/ethnicity. If such an association exists, it may suggest that sexual behavior stigma can be physiologically measured or indicated by the presence of heightened salivary cortisol. A subsample of 667 MSM participants from the 2019 American Men’s Internet Survey (AMIS; N = 10,129) submitted morning (AM) and evening (PM) saliva cortisol samples using at-home mail-in collection kits. Average daily cortisol and daily cortisol change were calculated; simple linear regressions estimated associations between cortisol measures and sexual behavior stigma characterized in four different ways (ever and recent experience of individual stigma items; average ever and recent experience of three stigma scales: stigma from family and friends, anticipated healthcare stigma, general social stigma). Participants reported a mean age of 36.0 years (SD = 14.9), with most being non-Hispanic white (n = 480, 72.0%), Hispanic (n = 164, 12.3%), or Black/African American (n = 146, 10.9%), and identified as homosexual/gay (n = 562, 84.3%). Reporting ever experiencing healthcare providers gossiping was significantly associated with higher PM cortisol (β = 0.12, p = 0.001) and higher average daily cortisol (β = 0.11, p = 0.004), while reporting ever experiencing police refusing to protect was associated with higher AM cortisol (β = 0.08, p = 0.03) and higher average daily cortisol (β = 0.09, p = 0.02). Recent experiences of stigma were not significant predictors of any measure of cortisol. Measures of salivary cortisol may be used to characterize sexual behavior stigma among MSM populations, however more insight is needed to determine its exact relationship and strength.
GWAS for the composite traits of hematuria and albuminuria
Authors: Gagliano Taliun Sarah A. , Dinsmore Ian R. , Mirshahi Tooraj , Chang Alexander R. , Paterson Andrew D. , Barua Moumita 
Published: October 2023

AbstractOur GWAS of hematuria in the UK Biobank identified 6 loci, some of which overlap with loci for albuminuria suggesting pleiotropy. Since clinical syndromes are often defined by combinations of traits, generating a combined phenotype can improve power to detect loci influencing multiple characteristics. Thus the composite trait of hematuria and albuminuria was chosen to enrich for glomerular pathologies. Cases had both hematuria defined by ICD codes and albuminuria defined as uACR > 3 mg/mmol. Controls had neither an ICD code for hematuria nor an uACR > 3 mg/mmol. 2429 cases and 343,509 controls from the UK Biobank were included. eGFR was lower in cases compared to controls, with the exception of the comparison in females using CKD-EPI after age adjustment. Variants at 4 loci met genome-wide significance with the following nearest genes: COL4A4, TRIM27, ETV1 and CUBN. TRIM27 is part of the extended MHC locus. All loci with the exception of ETV1 were replicated in the Geisinger MyCode cohort. The previous GWAS of hematuria reported COL4A3-COL4A4 variants and HLA-B*0801 within MHC, which is in linkage disequilibrium with the TRIM27 variant (D′ = 0.59). TRIM27 is highly expressed in the tubules. Additional loci included a coding sequence variant in CUBN (p.Ala2914Val, MAF = 0.014 (A), p = 3.29E−8, OR = 2.09, 95% CI = 1.61–2.72). Overall, GWAS for the composite trait of hematuria and albuminuria identified 4 loci, 2 of which were not previously identified in a GWAS of hematuria.
Full-optical photoacoustic imaging using speckle analysis and resolution enhancement by orthogonal pump patterns projection
Authors: Vorobev Viktor , Weidmann David , Agdarov Sergey , Beiderman Yafim , Shabairou Nadav , Benyamin Matan , Klämpfl Florian , Schmidt Michael , Gorin Dmitry , Zalevsky Zeev 
Published: October 2023

AbstractThis paper presents an approach for achieving full optical photoacoustic imaging with enhanced resolution utilizing speckle pattern analysis. The proposed technique involves projecting patterns derived from binary masks corresponding to orthogonal functions onto the target to elicit a photoacoustic signal. The resulting signal is then recorded using a high-speed camera and analyzed using correlation analysis of the speckle motion. Our results demonstrate the feasibility of this optical approach to achieve imaging with enhanced resolution without the need for physical contact with the target, opening up new possibilities for non-invasive medical imaging and other applications.