bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of a pharmacist‐driven chronic obstructive pulmonary disease transitions of care service at a large academic medical center
Authors: Lycouras Maggie M. , Cieri‐Hutcherson Nicole E. , Kersten Brian P. , Clark Collin M. 
Published: February 2024

AbstractIntroductionChronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality in the United States, and one in five COPD hospitalizations result in a readmission within 30 days. Pharmacists have been identified as key members of interdisciplinary teams to improve transitions of care.ObjectiveThe objective of this study was to develop, implement, and evaluate outcomes for a pharmacist‐driven COPD transitions of care bundle at an academic medical center.MethodsA COPD care bundle was implemented for patients presenting with a COPD exacerbation from December 1, 2021 to February 28, 2022. A historical control group was created from patients who were discharged between December 1, 2020 and February 28, 2021. Patient characteristics, pharmacist‐driven interventions, and time required for the intervention were assessed descriptively. Inhaler technique was assessed before and after pharmacist education utilizing standardized rubrics. The percentage change in inhaler scores was assessed with a Wilcoxon signed‐rank test. Readmission outcomes were analyzed using a chi‐squared test.ResultsThere were 30 patients in the intervention group and 46 in the control group. There were 104 interventions requiring provider collaboration, of which 84 (81%) were accepted. A median (interquartile range, IQR) of 46 (37–55) min was spent per patient in the intervention group. At baseline, patients scored a median of 84.6% (75–100) of steps correctly across all inhaler device types. After pharmacist education, patient scores increased to a median of 100% [92.3–100] (p < 0.0001). There were eight (26.7%) 30‐day all‐cause readmission rates in the intervention group and 15 (32.6%) in the control group (p = 0.58).ConclusionMost pharmacist recommendations were accepted by providers. Medication education led to improved understanding of inhaler technique, but there was no difference in 30‐day readmission rates. Further development of this service is warranted based on these findings.
On the antibacterial photodynamic inactivation mechanism of Emodin and Dermocybin natural photosensitizers: A theoretical investigation
Authors: Ritacca Alessandra Gilda , Prejanò Mario , Alberto Marta Erminia , Marino Tiziana , Toscano Marirosa , Russo Nino 
Published: February 2024

AbstractA DFT and TDDFT study has been carried out on monomeric anthraquinones Emodin and Dermocybin (Em, Derm) recently proposed as natural antibacterial photosensitizers able to act also against gram‐negative microbes. The computational study has been performed considering the relative amount of neutral and ionic forms of each compound in water, with the variation of pH. The occurrence of both Type I and Type II photoreactions has been explored computing the absorption properties of each species, the spin‐orbit coupling constants (SOC), the vertical ionization potentials and the vertical electron affinities. The most plausible deactivation channels leading to the population of excited triplet states have been proposed. Our data indicate Emodin as more active than Dermocybin in antimicrobial photodynamic therapy throughout the Type II mechanism. Our data support a dual TypeI/II activity of the monomeric anthraquinones Emodin and Dermccybin in water, in all the considered protonation states.
Bridging gaps: Transforming dental public health training for modern job market demands
Authors: Gritzer Lauren , Zavras Athanasios , Macek Mark , Alqaderi Hend 
Published: February 2024

AbstractObjectivesTo create optimal oral and overall community health, dental public health (DPH) specialists must be competent and appropriately skilled to deal with contemporary and emergent public health issues. This study aims to evaluate the current job market demands related to the DPH profession and suggest contemporary skills and qualities that would enable DPH professionals to address emerging issues more effectively in their field.MethodsA multimethod qualitative study was conducted involving two distinct groups: employers and potential employees. Interviews of stakeholders (employers) representing different job market domains, and a survey was administered to recent DPH residency graduates (employees). Qualitative analysis was applied to the data collected.ResultsEmployers identified the following skills as crucial for future DPH employees: proficiency in care delivery systems, health informatics, clinical training, leadership, and professional and grant writing skills. Many employers assumed the presence of a clinical component in the residency program. DPH residency graduates emphasized that the most significant barrier to securing positions is the lack of clinical experience within the residency program.ConclusionsThis research highlights skills currently valued by the DPH job market and proposes that DPH specialty training may consider revising its curriculum to include these skills. Implementing such updates would ensure that graduates are well equipped and competitive, thus significantly contributing to the broader objective of attaining optimal oral health and overall well‐being of the community.
Inclusion chromatic index of random graphs
Authors: Kwaśny Jakub , Przybyło Jakub 
Published: February 2024

AbstractErdős and Wilson proved in 1977 that almost all graphs have chromatic index equal to their maximum degree. In 2001 Balister extended this result and proved that the same number of colours is almost always sufficient if we additionally demand the distinctness of the sets of colours incident with any two vertices. We study a stronger condition and show that one more colour is almost always sufficient and necessary if the inclusion of these sets is forbidden for any pair of adjacent vertices. We also settle the value of a more restrictive graph invariant for almost all graphs, where inclusion is forbidden for all pairs of vertices, which necessitates one more colour for graphs of even order.
Novel oxadiazole functionalized pyridopyrimidine derivatives; their anticancer activity and molecular docking studies
Authors: Dasari Raghu , Gali Srinivas , Korra Rajashekar , Vaddiraju Namratha 
Published: February 2024

AbstractA series of novel oxadiazole functionalized pyridopyrimidine derivatives prepared starting from 6‐methyl/ethyl‐2‐oxo‐4‐(trifluoromethyl)‐1,2‐dihydropyridine‐3‐carbonitrile 1. This compound 1 on reaction with sulfuric acid obtained compound 2, further compound 2 on reaction with chloroacetamide followed by reaction with ethoxy methylene malonic diethyl ester coupling and further cyclization to obtain compound 5. Compound 5 on reaction with hydrazide hydrate obtained hydrazide derivatives 6. Compound 6 on reaction with diverse substituted aromatic acids to get oxadiazole derivatives 7a–l. All the final compounds 7a–l evaluated for anticancer activity against four human cancer cell lines such as HeLa—cervical cancer (CCL‐2); COLO 205—colon cancer (CCL‐222); HepG2—liver cancer (HB‐8065); and MCF7—breast cancer (HTB‐22) and promising compounds 7d and 7k have been identified and evaluated for molecular docking interactions.
Mind the misalignment: The moderating role of daily social sleep lag in employees' recovery processes
Authors: Völker Jette , Koch Theresa J. S. , Wiegelmann Monika , Sonnentag Sabine 
Published: February 2024

SummaryCircadian processes are important for employees and organizations yet have been relatively underexplored in recovery research. Thus, we embed the concept of circadian misalignment into the recovery literature by investigating the moderating role of employees' daily social sleep lag (i.e., a discrepancy between employees' actual and biologically preferred sleep–wake times) in their recovery processes. Building on the effort‐recovery model and a circadian perspective on recovery, we propose that low relaxation and mastery experiences explain the relationship between workplace interpersonal conflicts and low next‐morning vigor. Concerning circadian misalignment, we investigated whether daily social sleep lag impedes the occurrence and effectiveness of after‐work recovery experiences (i.e., moderates the relationships with interpersonal conflicts and vigor, respectively). Results of a daily diary study with 274 employees (1926 days) demonstrated that low mastery experiences, but not relaxation, explained the negative association between interpersonal conflicts and next‐morning vigor. Additionally, mastery experiences translated less to next‐morning vigor on days with high (vs. low) social sleep lag. Investigating circadian misalignment can thus help determine under which circumstances employees best recover from work, highlighting the need to take circadian processes into account in recovery research.
Impact of trial attrition rates on treatment effect estimates in chronic inflammatory diseases: A meta‐epidemiological study
Authors: Overgaard Silja H. , Moos Caroline M. , Ioannidis John P. A. , Luta George , Berg Johannes I. , Nielsen Sabrina M. , Andersen Vibeke , Christensen Robin 
Published: February 2024

AbstractThe objective of this meta‐epidemiological study was to explore the impact of attrition rates on treatment effect estimates in randomised trials of chronic inflammatory diseases (CID) treated with biological and targeted synthetic disease‐modifying drugs. We sampled trials from Cochrane reviews. Attrition rates and primary endpoint results were retrieved from trial publications; Odds ratios (ORs) were calculated from the odds of withdrawing in the experimental intervention compared to the control comparison groups (i.e., differential attrition), as well as the odds of achieving a clinical response (i.e., the trial outcome). Trials were combined using random effects restricted maximum likelihood meta‐regression models and associations between estimates of treatment effects and attrition rates were analysed. From 37 meta‐analyses, 179 trials were included, and 163 were analysed (301 randomised comparisons; n = 62,220 patients). Overall, the odds of withdrawal were lower in the experimental compared to control groups (random effects summary OR = 0.45, 95% CI, 0.41–0.50). The corresponding overall treatment effects were large (random effects summary OR = 4.43, 95% CI 3.92–4.99) with considerable heterogeneity across interventions and clinical specialties (I2 = 85.7%). The ORs estimating treatment effect showed larger treatment benefits when the differential attrition was more prominent with more attrition in the control group (OR = 0.73, 95% CI 0.55–0.96). Higher attrition rates from the control arm are associated with larger estimated benefits of treatments with biological or targeted synthetic disease‐modifying drugs in CID trials; differential attrition may affect estimates of treatment benefit in randomised trials.
Production optimization of food functional factor ergothioneine in wild‐type red yeast Rhodotorula mucilaginosaDL‐X01
Authors: Xiong Kexin , Guo Hui , Xue Siyu , Liu Mengyang , Dai Yiwei , Lin Xinping , Zhang Sufang 
Published: February 2024

AbstractBACKGROUNDErgothioneine (EGT) is a high‐value food functional factor that cannot be synthesized by humans and other vertebrates, and the low yield limits its application.RESULTSIn this study, the optimal fermentation temperature, fermentation time, initial pH, inoculum age, and inoculation ratio on EGT biosynthesis of Rhodotorula mucilaginosa DL‐X01 were optimized. In addition, the effects of three key precursor substances – histidine, methionine, and cysteine – on fungal EGT synthesis were verified. The optimal conditions were further obtained by response surface optimization. The EGT yield of R. mucilaginosa DL‐X01 under optimal fermentation conditions reached 64.48 ± 2.30 mg L−1 at shake flask fermentation level. Finally, the yield was increased to 339.08 ± 3.31 mg L−1 (intracellular) by fed‐batch fermentation in a 5 L bioreactor.CONCLUSIONTo the best of our knowledge, this is the highest EGT yield ever reported in non‐recombinant strains. The fermentation strategy described in this study will promote the efficient biosynthesis of EGT in red yeast and its sustainable production in the food industry. © 2024 Society of Chemical Industry.
Proposal for standardized ultrasound analysis of the salivary glands: Part 1 submandibular gland
Authors: Hoffman Henry T. , Koch Michael , Witt Robert Lee , Ryan William R. , Zenk Johannes , Katz Philippe , Rahmati Rahmatullah , Rassekh Christopher , Donato Francisco , McCulloch Timothy M. , Joshi Arjun S. , Chang Jolie Lien , Gillespie M. Boyd , Pichardo Priscilla F. A. , Orloff Lisa Ann , Marcelino Antonio , Wenzel Piper , Cohen David , Fundakowski Christopher E. , Cognetti David M. , Walvekar Rohan R. , Bertelli Antonio , Quon Harry , Anderson Carryn , Policeni Bruno , Siegel Gordy 
Published: February 2024

AbstractObjectivesThe Salivary Gland Committee of the American Academy of Otolaryngology‐Head and Neck Surgery seeks to standardize terminology and technique for ultrasonograpy used in the evaluation and treatment of salivary gland disorders.MethodsDevelopment of expert opinion obtained through interaction with international practitioners representing multiple specialties. This committee work includes a comprehensive literature review with presentation of case examples to propose a standardized protocol for the language used in ultrasound salivary gland assessment.ResultsA multiple segment proposal is initiated with this focus on the submandibular gland. We provide a concise rationale for recommended descriptive language highlighted by a more extensive supplement that includes an extensive literature review with additional case examples.ConclusionRecommendations are provided to improve consistency both in performing and reporting submandibular gland ultrasound.
HNRNPC promotes estrogen receptor‐positive breast cancer cell cycle by stabilizing WDR77 mRNA in an m6A‐dependent manner
Authors: Xu Wenjie , Huang Ziwei , Xiao Yunxiao , Li Wenhui , Xu Ming , Zhao Qiuyang , Yi Pengfei 
Published: February 2024

AbstractBreast cancer has become the most commonly diagnosed cancer. Heterogeneous nuclear ribonucleoprotein C (HNRNPC), a reader of N6‐methyladenosine (m6A), has been observed to be upregulated in various types of cancer. Nevertheless, the role of HNRNPC in breast cancer and whether it is regulated by m6A modification deserve further investigation. The expression of HNRNPC in breast cancer was examined by quantitative real‐time polymerase chain reaction and western blot analysis. RNA immunoprecipitation was performed to validate the binding relationships between HNRNPC and WD repeat domain 77 (WDR77). The effects of HNRNPC and m6A regulators on WDR77 were investigated by actinomycin D assay. The experiments in vivo were conducted in xenograft models. In this research, we found that HNRNPC was highly expressed in breast cancer, and played a crucial role in cell growth, especially in the luminal subtype. HNRNPC could combine and stabilize WDR77 mRNA. WDR77 successively drove the G1/S phase transition in the cell cycle and promoted cell proliferation. Notably, this regulation axis was closely tied to the m6A modification status of WDR77 mRNA. Overall, a critical regulatory mechanism was identified, as well as promising targets for potential treatment strategies for luminal breast cancer.
KDM5B promotes metastasis and epithelial–mesenchymal transition via Wnt/β‐catenin pathway in squamous cell carcinoma of the head and neck
Authors: Yuan Wenhui , Hu Junli , Wang Mengshu , Li Guo , Lu Shanhong , Qiu Yuanzheng , Liu Chao , Liu Yong 
Published: February 2024

AbstractMetastasis determines clinical management decision and restricts the therapeutic efficiency in patients with squamous cell carcinoma of the head and neck (SCCHN). Epigenetic factor KDM5B serves as an oncogene in multiple cancers. However, its role in SCCHN metastasis remains unclear. Our previous study showed that KDM5B is significantly elevated in SCCHN tissue and is positively correlated with metastasis and recurrence. KDM5B overexpression predicted a poor prognosis in both disease‐free survival and overall survival, which served as an independent prognostic factor in SCCHN patients. This study further investigates the exact impact of KDM5B in metastasis of SCCHN. We found that KDM5B knockdown significantly inhibits the migration and invasion of SCCHN cells both in vitro and in vivo. On the contrary, forced expression of KDM5B leads to enhanced migration and invasion, accompanied by canonical alterations of epithelial–mesenchymal transition (EMT). Mechanism investigations demonstrated that KDM5B activates Wnt/β‐catenin pathway, and inhibition of Wnt/β‐catenin pathway via a small molecule inhibitor iCRT‐14 partially reverses the enhanced migratory and invasive ability caused by KDM5B in SCCHN cells. Together, our data indicate that KDM5B promotes EMT and metastasis via Wnt/β‐catenin pathway in SCCHN, suggesting that KDM5B may be a potential therapeutic target and prognosis biomarker in SCCHN.
Peptidase inhibitor 16 promotes proliferation of pancreatic ductal adenocarcinoma cells through OASL signaling
Authors: Chen Qun , Jiang Lu‐Yang , Cao Cheng , Liu Feng‐Yuan , Li Dan‐Rui , Wu Peng‐Fei , Jiang Kui‐Rong 
Published: February 2024

AbstractPancreatic ductal adenocarcinoma (PDAC) is a highly invasive cancer with a poor prognosis and a 5‐year survival rate of less than 11%. As a member of the CAP superfamily of proteins, the role of peptidase inhibitor 16 (Pi16) in tumor progression is still unclear. Immunohistochemistry and quantitative RT‐PCR methods were used to detect the expression levels of Pi16 protein and mRNA in PDAC patients. CRISPR/Cas9 technology was used to knock out the expression of Pi16 in PDAC cell lines. In vivo and in vitro experiments were used to verify the effect of Pi16 on PDAC proliferation ability. By RNA sequencing, we found that oligoadenylate synthetase L (OASL) can serve as a potential downstream target of Pi16. The expression of Pi16 was higher in PDAC tissues than in matched adjacent tissues. High expression of Pi16 was associated with PDAC progression and poor prognosis. Overexpression of Pi16 could promote the proliferation of PDAC cells in vitro and in vivo. Bioinformatics analysis and coimmunoprecipitation assays showed that Pi16 could bind to OASL. Moreover, the functional recovery test confirmed that Pi16 could promote the proliferation of PDAC via OASL. Our present study demonstrates that Pi16 might participate in the occurrence and development of PDAC by regulating cell proliferation by binding to OASL, indicating that Pi16 might be a promising novel therapeutic target for PDAC.
Clinical details of individuals with Rauch–Steindl syndrome due to NSD2 truncating variants
Authors: Nishi Eriko , Yanagi Kumiko , Kaname Tadashi , Okamoto Nobuhiko 
Published: February 2024

AbstractBackgroundRauch–Steindl syndrome (RAUST) is a very rare genetic syndrome caused by a pathogenic variant in NSD2 on chromosome 4p16.3. Although NSD2 was previously thought to be the major gene in Wolf–Hirschhorn syndrome (WHS), a contiguous gene syndrome of chromosome 4p16.3 deletion, RAUST has been found to present different facial and clinical features from WHS. In this study, we report the details of two newly diagnosed individuals with RAUST in order to better understand the molecular and clinical features of RAUST.MethodsWhole‐genome sequencing was performed on two individuals with psychomotor delay and growth failure. Detailed clinical evaluation of growth parameters, craniofacial features, electroencephalogram (EEG), magnetic resonance imaging of the brain, and developmental assessment were performed.ResultsBoth individuals had de novo truncating variants in NSD2. One had a novel variant (c.2470C>T, p.Arg824*), and the other had a recurrent variant (c.4028del, p.Pro1343Glnfs*49). Both exhibited characteristic RAUST facial features, growth failure, and mild psychomotor delay. A novel finding of RAUST was seen in individual 2, a Chiari malformation type 1, and both showed delayed bone age. They lacked common WHS features such as congenital heart defects, cleft lip/palate, and seizures (EEG with abnormal findings).ConclusionWe present a novel variant and clinical presentations of RAUST, expand the molecular and clinical diversity of RAUST, and improve our understanding of this rare syndrome, which is distinct from WHS. Further researches are needed on more RAUST cases and on functional analysis of NSD2.
Antenatal description of large 4q13.2q21.23 deletion and outcomes
Authors: Giguet‐Valard Anna‐Gaëlle , Thevenin Christelle , Dreux Sophie , Decatrelle Valérie , Juve Marie‐Laure , Yazza Soraya , Adenet Clara , Lesueur Marion , Bouvagnet Patrice , Gueneret Michèle 
Published: February 2024

AbstractBackground4q21 microdeletion syndrome is an emergent non‐recurrent genomic disorder characterized by facial dysmorphy, progressive growth retardation, severe intellectual deficit, and absent or severely delayed speech. Deletions occur in clusters along 4q interstitial or terminal regions. 4q chromosomal aberrations are variable in type, size, and breakpoint. Genotype–phenotype correlation is a challenging task. The recurrent antenatal feature associated a posteriori with this syndrome is intrauterine growth retardation. There are very few precise antenatal descriptions of this syndrome.MethodsWe report here the first antenatal history of one of the largest deletion of this region.ResultsOur case harbored a 16.9 Mb deletion encompassing 135 protein coding genes including 20 OMIM morbid genes involved in neurological and cognitive abilities. Those breakpoints overlap two clusters of described microdeletion syndromes of cytogenetic band 4q13 and 4q21.ConclusionFrom the end of the second trimester, set of call signs associated with this syndrome can be completed by: excess of amniotic fluid, mild growth retardation, short long bones, bony anomalies of the extremities, and bulging cheeks. So, emphasis should be placed on the examination of the extremities, and the face during the routine targeted prenatal ultrasound.