bluuebunny/crossref_metadata_2025_split huggingface dataset:
КРИМІНАЛЬНЕ ПРОВАДЖЕННЯ В УМОВАХ ВОЄННОГО СТАНУ
Authors: Любавіна В. П. , Смородінова М. В. 
Published: December 2023

У статті досліджується явище режиму воєнного стану. Розглянуто поняття воєнного стану. Проаналізовано чинне законодавство в контексті регулювання здійснення кримінального провадження в умовах воєнного стану.
В умовах ведення повномасштабної війни дослідження ведення кримінального провадження в умовах особливого стану набуває усе більшого значення. Останнім часом розширюється коло внесених змін до Кримінального процесуального кодексу Укрвїни у частині регулювання цього явища. Досудове розслідування за таких умов зазнало істотних змін, однак виникає багато дискусійних питань і непорозумінь, коли йдеться про порядок проведення та процесуальні строки. Це зі свого боку зумовлює актуальність теми та необхідність ретельного вивчення законодавчої бази, аналізу проблемних питань, які виникають у зв’язку з цим. Тому в умовах збільшення вчинення кримінально-караних дій вкрай важливо аби кримінальне процесуальне законодавство України давало змогу розслідувати та регламентувало цей процес.
Особливостям здійснення досудового розслідування в умовах воєнного стану виді­лено розділ ІХ-1 КПКУкраїни, статті якого потрібно враховувати у процесі захисту осіб у кримінальному провадженні. Але зміни було внесено і в статті інших розділів.
Досліджено зміни у фіксації перебігу та результатів проведення слідчих (розшукових) дій. Аргументовано необхідність дистанційної участі захисника у процесуальній дії. Проаналізовано строки повідомлення про підозру затриманому в умовах воєнного стану. Обґрунтовано збільшення часу проведення слідчих дій та строки повідомлення особі про підозру. Розглянуто питання про створення міжвідомчих слідчих груп. Звернено увагу на переклад стороною обвинувачення, строк затримання особи, скасування або зміну запобіжного заходу, поновлення пропущеного строку, доручення на здійснення досудового розслідування. Зазначено, що обов’язковим є зберігання матеріалів кримінального провадження в електронній формі й у разі потреби існує можливість відновлення втрачених матеріалів.
Доведено необхідність внесення змін до законодавства з метою забезпечити нормальну діяльність правоохоронних органів в умовах воєнного стану.
Sintesis Dan Karakterisasi Material Katoda LiMn0,7Fe0,3-xNixPO4/C Dengan 0 ≤ X ≤ 0,2 Dalam Aplikasi Baterai Litium-Ion
Authors: Rudiyansah Rudiyansah , S Anne Zulfia , Prihandoko Bambang 
Published: December 2023

Pati singkong sebagai karbon pelapis bahan aktif LiMnPO4, Fe dan Ni (covalent-doping) ditempuh untuk meningkatkan konduktifitas elektronik-ionik, nilai specific capacity dan working voltage. Proses solid state reaction berupa ball milling (330 rpm, 48 jam) dan sintering (800°C, 2 jam) digunakan dalam pembuatan bahan aktif. Pola difraksi LiMnPO4 terlihat pada uji XRD pada bahan aktif. Bahan aktif terlapisi karbon berukuran 290 nm dan ukuran kristalit 60 nm terbentuk melalui proses Ball Milling. Pengujian SEM memperlihatkan pertumbuhan pelapisan karbon kearah horizontal dan pengujian EDX menunjukkan kadar Mn yang tinggi mengkonfirmasi peran pati singkong sebagai fasilitator pengintian pelapisan karbon. Nilai vibrasi v1 - v4 (1138 dan 1098 cm-1) hasil pengujian FTIR menunjukkan polianion terbentuk. Pelapisan karbon memberikan nilai konduktifitas elektronik-ionik sebesar 1 x 10-3 S/cm dan 7,2 S/cm, peningkatan nilai konduktifitas elektronik terjadi akibat penambahan Ni. LiMn0,7Fe0,25Ni0,05PO4/C memberikan nilai specific capacity oksidasi 60,92 mAh/gr dan nilai Voks-red sekitar 4,13 Volt dan secara praktikal dapat digunakan sebagai bahan aktif katoda baterai Li-Ion.
ABSTRACT
Carbon-coating process with starch of cassava in LiMnPO4 active material, co-subtitution by adding Fe and Ni have been used to enhance ionic-electronic conductivity, specific capacity, and working voltage. Pattern diffraction of XRD shown LiMnPO4 structure have been formed via milling process (330 rpm, 48 hours) and sintering process (800°C, 2 hours) as called as solid state reaction. Ball Milling produced active material with the particle size and crystallite size up to 290 nm and 60 nm respectively. Carbon-coating have been grown in horizontal direction in cathode material become an evidence that the starch of cassava have been facilitates nuclea of carbon-coating to grown in cathode material and can be seen by SEM, and also the high content of Mn that have founded by EDX evaluation agreed. Polyanion have formed and indicated by vibration value of v1 - v4 (1138 and 1098 cm-1) during FTIR evaluation. Electronic conductivity increased up to 1 x 10-3 S/cm by carbon-adding process, and Ni-addition as cation-doping contributed also. LiMn0.7Fe0,25Ni0.05PO4/C of cathode material shown the highest specific capacity oxidation near 60.92 mAh/gr and Voxidation/reduction around 4.13 Volts and practically can be used as Li-Ion battery.
Weed control with saturated steam in organic highbush blueberry
Authors: Moretti Marcelo L. , Pedroso Rafael M. 
Published: December 2023

Weed management is often a predominant and costly problem in the production of organic blueberries. Geotextile weed fabrics of woven polyethylene are widely used in organic blueberry fields to suppress weeds growing within the rows. Weeds, such as Convolvulus arvensis L., grow at the base of the blueberry plants or through openings and around the edges of the weed fabric, thus requiring hand weeding. This study evaluates the integration of saturated steam (SS), a rotary brush (RB), and organic herbicides for weed control in blueberries. Dose–response studies indicated that SS applied at 121°C and at 7.4 m3 ha−1 of steam (3,655 MJ ha−1) resulted in over 90% control and a reduction in the dry weights of C. arvensis. When treatments were directed to the base of the blueberry plants, SS at 7.4 m3 ha−1 provided 80% control of C. arvensis 28 days after treatment (DAT) and was comparable to hand weeding. Both of these treatments outperformed capric plus caprylic acid (CC) (33.2 kg ai ha−1) or ammonium nonanoate (AN) (24.3 kg ai ha−1) applications, despite C. arversis regrowth being observed. Four repetitive basal applications of SS of up to 29.6 m3 ha−1 over two consecutive years caused minimal and transient damage to new basal shoots of ‘Elliot’ and ‘Duke’ blueberries; basal shoot cross-sectional area compared with the non-treated was unaffected. In contrast, basal application of AN treatments damaged or killed basal shoots. When treatments were applied to the edge of the weed fabric, SS (7.4 m3 ha−1) reduced weed biomass by 42% to 93% at 28 DAT compared with the non-treated. The RB treatment reduced weed biomass from 72% to 99% in all experiments, while CC and AN reduced biomass by 18% to 54%. A partial budget analysis indicated that SS and the RB were 3- and 6.5-fold less expensive than organic herbicides, respectively. Integrating physical (SS) and mechanical (RB) treatments improved weed control. The latter, however, damaged the weed-suppressing fabric where preexisting holes were present, generated dust, and increased the chance of fruit contamination. The SS was safe for the weed-suppressing fabric and the blueberry, but weed regrowth following treatment and copious water requirements hindered its feasibility.
UrbanGenoGAN: pioneering urban spatial planning using the synergistic integration of GAN, GA, and GIS
Authors: Cheng Wanyue , Chu Yusu , Xia Chenyuan , Zhang Boliang , Chen Junming , Jia Mengyan , Wang Wenxiao 
Published: December 2023

Introduction: Urban spatial planning is critical for the development of sustainable and livable cities. However, traditional planning methods often face challenges in handling complex planning scenarios and large-scale data.Methods: This paper introduces UrbanGenoGAN, a novel algorithm that integrates generative adversarial networks (GANs), genetic optimization algorithms (GOAs), and geographic information system (GIS) to address these challenges. Leveraging the generative power of GANs, the optimization capabilities of genetic algorithms, and the spatial analysis capabilities of GIS, UrbanGenoGAN is designed to generate optimized urban plans that cater to various urban planning challenges. Our methodology details the algorithm’s design and integration of its components, data collection and preprocessing, and the training and implementation processes.Results: Through rigorous evaluation metrics, comparative analysis with existing methodologies, and case studies, the proposed algorithm demonstrates significant improvement in urban planning outcomes. The research also explores the technical and practical considerations for implementing UrbanGenoGAN, including scalability, computational efficiency, data privacy, and ethical considerations.Discussion: The findings suggest that the integration of advanced machine learning and optimization techniques with spatial analysis offers a promising approach to enhancing decision-making in urban spatial planning. This work contributes to the growing field of AI applications in urban planning and paves the way for more efficient and sustainable urban development.
Case report: Prenatal diagnosis in the fetus of a couple with both thalassemia and deafness genes
Authors: Li Youqiong , Liang Liang , Bai Jinping , Zheng Lihong , Qin Ting 
Published: December 2023

Background: Prenatal diagnosis and genetic counseling play an important role in preventing and controlling birth defects. No reports were found of prenatal diagnosis of couples carrying both the thalassemia and deafness genes. In this study, we presented the prenatal screening and diagnosis of a couple with both thalassemia and deafness genes, contributing to better genetic counseling.Case Report: A couple visited our hospital for a routine prenatal examination. As required by the policy in our region, they underwent screening and genetic diagnosis for thalassemia. Meanwhile, they did not accept the recommendation to test for spinal muscular atrophy and deafness genes. The female was confirmed to be a Hb Quong Sze (Hb QS) carrier (αQSα/αα, βN/βN), and the male had Hb H disease combined with β-thalassemia (--SEA/αCSα, βCDs41-42 (-TTCT)/βN). A prenatal diagnosis of the fetus revealed a Hb CS heterozygote. Subsequent complementary testing showed that the male was a double heterozygote of the GJB2 gene c.299_300delAT combined with c.109G&gt;A, and Sanger sequencing confirmed that the female was a carrier of c.508_511dup in the GJB2. Fortunately, the chorionic villi results indicated that the fetus was only a carrier of deafness.Conclusion: Since both partners carried thalassemia and deafness genes, the couple required prenatal diagnosis for the respective mutations. Expanded carrier screening (ECS) is a more advanced technology that can detect multiple disease genes simultaneously.
Deciphering the human antibody response against Burkholderia pseudomallei during melioidosis using a comprehensive immunoproteome approach
Authors: Wagner Gabriel E. , Stanjek Thomas Franz Paul , Albrecht Dirk , Lipp Michaela , Dunachie Susanna J. , Föderl-Höbenreich Esther , Riedel Katharina , Kohler Anne , Steinmetz Ivo , Kohler Christian 
Published: December 2023

IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis. Antigens inducing protective immunity in experimental models have recently been identified and serodiagnostic tools have been improved. However, further elucidation of the antigenic repertoire of B. pseudomallei during human infection for diagnostic and vaccine purposes is required. The adaptation of B. pseudomallei to very different habitats is reflected by a huge genome and a selective transcriptional response to a variety of conditions. We, therefore, hypothesized that exposure of B. pseudomallei to culture conditions mimicking habitats encountered in the human host might unravel novel antigens that are recognized by melioidosis patients.Methods and resultsIn this study, B. pseudomallei was exposed to various stress and growth conditions, including anaerobiosis, acid stress, oxidative stress, iron starvation and osmotic stress. Immunogenic proteins were identified by probing two-dimensional Western blots of B. pseudomallei intracellular and extracellular protein extracts with sera from melioidosis patients and controls and subsequent MALDI-TOF MS. Among B. pseudomallei specific immunogenic signals, 90 % (55/61) of extracellular immunogenic proteins were identified by acid, osmotic or oxidative stress. A total of 84 % (44/52) of intracellular antigens originated from the stationary growth phase, acidic, oxidative and anaerobic conditions. The majority of the extracellular and intracellular protein antigens were identified in only one of the various stress conditions. Sixty-three immunoreactive proteins and an additional 38 candidates from a literature screening were heterologously expressed and subjected to dot blot analysis using melioidosis sera and controls. Our experiments confirmed melioidosis-specific signals in 58 of our immunoproteome candidates. These include 15 antigens with average signal ratios (melioidosis:controls) greater than 10 and another 26 with average ratios greater than 5, including new promising serodiagnostic candidates with a very high signal-to-noise ratio.ConclusionOur study shows that a comprehensive B. pseudomallei immunoproteomics approach, using conditions which are likely to be encountered during infection, can identify novel antibody targets previously unrecognized in human melioidosis.
Evaluating real-patient learning in medical education – Hungarian validation of the Manchester Clinical Placement Index
Authors: Fábry Szabolcs , Rózsa Sándor , Hargittay Csenge , Kristóf Petra , Szélvári Ágnes , Vörös Krisztián , Torzsa Péter , Németh Endre , Dornan Timothy , Eőry Ajándék 
Published: December 2023

IntroductionThe Manchester Clinical Placement Index (MCPI) is an instrument to measure medical undergraduates’ real-patient learning in communities of practice both in hospital and in GP placements. Its suitability to evaluate the quality of placement learning environments has been validated in an English-language context; however, there is a lack of evidence for its applicability in other languages. Our aim was to thoroughly explore the factor structure and the key psychometric properties of the Hungarian language version.MethodsMCPI is an 8-item, mixed-method instrument which evaluates the quality of clinical placements as represented by the leadership, reception, supportiveness, facilities and organization of the placement (learning environment) as well as instruction, observation and feedback (training) on 7-point Likert scales with options for free-text comments on the strengths and weaknesses of the given placement on any of the items. We collected data online from medical students in their preclinical (1st, 2nd) as well as clinical years (4th, 5th) in a cross-sectional design in the academic years 2019–2020 and 2021–2022, by the end of their clinical placements. Our sample comprises data from 748 medical students. Exploratory and confirmatory factor analyses were performed, and higher-order factors were tested.ResultsAlthough a bifactor model gave the best model fit (RMSEA = 0.024, CFI = 0.999, and TLI = 0.998), a high explained common variance (ECV = 0.82) and reliability coefficients (ωH = 0.87) for the general factor suggested that the Hungarian version of the MCPI could be considered unidimensional. Individual application of either of the subscales was not supported statistically due to their low reliabilities.DiscussionThe Hungarian language version of MCPI proved to be a valid unidimensional instrument to measure the quality of undergraduate medical placements. The previously reported subscales were not robust enough, in the Hungarian context, to distinguish, statistically, the quality of learning environments from the training provided within those environments. This does not, however, preclude formative use of the subscales for quality improvement purposes.
Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses
Authors: Bourebaba Lynda , Kępska Martyna , Qasem Badr , Zyzak Magdalena , Łyczko Jacek , Klemens Marta , Mularczyk Malwina , Marycz Krzysztof 
Published: December 2023

Equine metabolic syndrome (EMS) is a steadily growing endocrine disorder representing a real challenge in veterinary practice. As a multifactorial condition, EMS is characterized by three main metabolic abnormalities including insulin resistance, increased adiposity or obesity and hoof laminitis. Adipose tissue dysfunction is recognized as a core pathophysiological determinant of EMS, as it strongly participates to lipotoxicity and systemic metaflammation, both of which have been closely linked to the development of generalized insulin resistance. Besides, sex hormone binding globulin (SHBG) is an important sex steroids transporters that has been recently proposed as an important metabolic mediator. Therefore, the aim of this study was to verify whether SHBG treatment may ameliorate subcutaneous adipose tissue metabolic failure under EMS condition in terms of lipidome homeostasis, lipid metabolism programs, insulin signalling and local inflammation. Subcutaneous adipose tissue (SAT) biopsies were collected post-mortem from healthy (n = 3) and EMS (n = 3) slaughtered horses. SHBG protein has been applied to SAT samples from EMS horses for 24 h at a final concentration of 50 nM, while control groups (healthy and untreated EMS) were cultured in the presence of SHBG-vehicle only. Tissues from all groups were afterwards secured for downstream analysis of gene expression using RT-qPCR, protein levels by Western blot and ELISA assay and lipidomics through GC-MS technique. Obtained results showcased that SHBG intervention efficiently normalized the altered fatty acids (FAs) profiles by lowering the accumulation of saturated and trans FAs, as well as the pro-inflammatory arachidonic and linoleic acids. Moreover, SHBG showed promising value for the regulation of adipocyte lipolysis and engorgement by lowering the levels of perilipin-1. SHBG exerted moderated effect toward SCD1 and FASN enzymes expression, but increased the LPL abundance. Interestingly, SHBG exhibited a negative regulatory effect on pro-adipogenic stimulators and induced higher expression of KLF3, IRF3 and β-catenin, known as strong adipogenesis repressors. Finally, SHBG protein showed remarkable ability in restoring the insulin signal transduction, IR/IRS/Pi3K/AKT phosphorylation events and GLUT4 transporter abundance, and further attenuate pro-inflammatory response by lowering IL-6 tissue levels and targeting the PDIA3/ERK axis. Overall, the obtained data clearly demonstrate the benefice of SHBG treatment in the regulation of adipose tissue metabolism in the course of EMS and provide new insights for the development of molecular therapies with potential translational application to human metabolic disorders.
Formation of dsRNA by-products during in vitro transcription can be reduced by using low steady-state levels of UTP
Authors: Ziegenhals Thomas , Frieling Ronja , Wolf Philipp , Göbel Katharina , Koch Stina , Lohmann Mia , Baiersdörfer Markus , Fesser Stephanie , Sahin Ugur , Kuhn Andreas N. 
Published: December 2023

Introduction: Exogeneous messenger ribonucleic acid (mRNA) can be used as therapeutic and preventive medication. However, during the enzymatic production process, commonly called in vitro transcription, by-products occur which can reduce the therapeutic efficacy of mRNA. One such by-product is double-stranded RNA (dsRNA). We therefore sought to limit the generation of dsRNA by-products during in vitro transcription.Materials and methods:In vitro transcription was performed with a DNA template including a poly(A)-tail-encoding region, dinucleotide or trinucleotide cap analogs for cotranscriptional capping, and relevant nucleoside triphosphates. Concentrations of UTP or modified UTP (m1ΨTP) and GTP were reduced and fed over the course of the reaction. mRNA was analyzed for dsRNA contamination, yield of the reaction, RNA integrity, and capping efficiency before translational activity was assessed.Results: Limiting the steady-state level of UTP or m1ΨTP during the enzymatic reaction reduced dsRNA formation, while not affecting mRNA yield or RNA integrity. Capping efficiency was optimized with the use of a combined GTP and UTP or m1ΨTP feed, while still reducing dsRNA formation. Lower dsRNA levels led to higher protein expression from the corresponding mRNAs.Discussion: Low steady-state concentrations of UTP and GTP, fed in combination over the course of the in vitro transcription reaction, produce mRNA with high capping and low levels of dsRNA formation, resulting in high levels of protein expression. This novel approach may render laborious purification steps to remove dsRNA unnecessary.
Therapeutic effect of ofatumumab in patients with myasthenia gravis: immunoregulation of follicular T helper cells and T helper type 17 cells
Authors: Li Shasha , Zhang Zhaoxu , Liu Zunjing 
Published: December 2023

IntroductionThis study aimed to study the therapeutic effects of ofatumumab in patients with myasthenia gravis (MG) in addition to the immunomodulatory effects on peripheral follicular T helper (Tfh) cells and T helper type 17 (Th17) cells.MethodsThirty-one patients with anti-acetylcholine receptor (AChR) antibody-positive MG were included in this study. At weeks 0, 1, 2, and 4, an initial dose of 20 mg of ofatumumab was injected subcutaneously, with a 2-month follow-up after completing this first cycle. At baseline, 1 month, and 3 months, we assessed the Quantitative MG (QMG), 15-item MG-Quality of Life (MG-QOL15), and MG-Activities of Daily Living (MG-ADL) scales and measured the frequencies of Tfh, Th17, and B cells and the levels of anti-AChR antibody, IL-6, IL-21, and IL-17 in the peripheral blood.ResultsAt 1 month and 3 months, the QMG, MG-QOL15, and MG-ADL scores were all significantly reduced. At 3 months, doses of prednisone were reduced by an average of 37%. Decreased frequencies of Tfh and Th17 cells, depletion of B cells, and reduced levels of IL-6, IL-21, and IL-17 were all observed at 1 month or 3 months.DiscussionTherefore, the therapeutic effect of ofatumumab could be detected after one cycle of treatment, which was maintained for 2 months. The immunomodulatory effect of ofatumumab during the observation period may involve depletion of B cells, reduction of Tfh and Th17 cells frequencies, and reduced levels of IL-6, IL-21, and IL-17. The findings provide novel data for the potential application of ofatumumab in MG.