bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimizing the Dutch newborn screening for congenital hypothyroidism by incorporating amino acids and acylcarnitines in a machine learning-based model
Authors: Jansen Heleen I , van Haeringen Marije , Bouva Marelle J , den Elzen Wendy P J , Bruinstroop Eveline , van der Ploeg Catharina P B , van Trotsenburg A S Paul , Zwaveling-Soonawala Nitash , Heijboer Annemieke C , Bosch Annet M , de Jonge Robert , Hoogendoorn Mark , Boelen Anita 
Published: October 2023

Objective
Congenital hypothyroidism (CH) is an inborn thyroid hormone (TH) deficiency mostly caused by thyroidal (primary CH) or hypothalamic/pituitary (central CH) disturbances. Most CH newborn screening (NBS) programs are thyroid-stimulating-hormone (TSH) based, thereby only detecting primary CH. The Dutch NBS is based on measuring total thyroxine (T4) from dried blood spots, aiming to detect primary and central CH at the cost of more false-positive referrals (FPRs) (positive predictive value (PPV) of 21% in 2007–2017). An artificial PPV of 26% was yielded when using a machine learning-based model on the adjusted dataset described based on the Dutch CH NBS. Recently, amino acids (AAs) and acylcarnitines (ACs) have been shown to be associated with TH concentration. We therefore aimed to investigate whether AAs and ACs measured during NBS can contribute to better performance of the CH screening in the Netherlands by using a revised machine learning-based model.

Methods
Dutch NBS data between 2007 and 2017 (CH screening results, AAs and ACs) from 1079 FPRs, 515 newborns with primary (431) and central CH (84) and data from 1842 healthy controls were used. A random forest model including these data was developed.

Results
The random forest model with an artificial sensitivity of 100% yielded a PPV of 48% and AUROC of 0.99. Besides T4 and TSH, tyrosine, and succinylacetone were the main parameters contributing to the model’s performance.

Conclusions
The PPV improved significantly (26–48%) by adding several AAs and ACs to our machine learning-based model, suggesting that adding these parameters benefits the current algorithm.
Ecological experimentations
Authors: Murari Lucas 
Published: October 2023

The goal of this paper is to highlight the ecological aspect in the works of fi lmmaker Rose Lowder. Her fi lms are shot outdoors, in nature, and aim to modify visual perception and image-based representation, foregrounding the materiality of the fi lmic medium. Her fi lmography, characterized by its use of unique shooting and editing techniques, is remarkable for its calculation regarding creative possibilities. The parameters of investigation outlined in this study are the fi lmic procedures that stress the aesthetic and ecological qualities of this artist’s work
Pedunculate oak (Quercus robur L.) in Bosnia and Herzegovina - state and perspectives
Authors: HODŽİĆ Mirzeta Memišević , BALLİAN Dalibor 
Published: October 2023

Bosnia and Herzegovina is located in Southeast Europe, and more than half its area of 51.129 km² belongs to forests and forest lands. 93% of the forests of Bosnia and Herzegovina are natural forests and have many plant species, many of which are endemic. There are eight oak species growing in Bosnia and Herzegovina. The most important ones are Quercus robur (despite being almost extinct due to over-exploitation during the late XIX and early XX century), and Quercus petraea. Pedunculate oak in Bosnia and Herzegovina is found in mixed stands with common hornbeam and in pure stands under exceptional conditions, with a total area of about 30,000 ha. In this paper, the data of the studies carried out in the pedunculate oak forests of Bosnia and Herzegovina were compiled and a general evaluation was made. The results of phenological observation in pedunculate oak provenance test in Bosnia and Herzegovina through the years showed statistically significant differences among provenances in the beginning, end and duration of individual phenological phases. Researches on growth showed overtaking of provenances through the years, and best results in provenance Jelah for the most of investigated years. The research results on showed a significant positive correlation between the height and root collar diameter in provenance test and most of the investigated .The remaining pedunculate oak populations in Bosnia and Herzegovina have a good genetic structure and can be used as a seed stands for collecting seed material and producing seedlings for afforestation with this species.
Continuous ultraviolet irradiation induces the development of irreversible resistance of melanoma cells to anticancer drugs
Authors: Shchegolev Yu. Yu. , Karpukhina M. A. , Sorokin D. V. , Scherbakov A. M. , Andreeva O. E. , Razuvaeva V. E. , Bogush T. A. , Mikhaylova I. N. , Demidov L. V. , Gudkova M. V. , Krasil’nikov M. A. 
Published: October 2023

Introduction. Melanoma belongs to the group of the most malignant tumors characterized by aggressive growth and active metastasis. At the same time, the effectiveness of therapy, primarily targeted therapy, is largely limited by the rapid development of drug resistance.Aim. To study the effect of chronic ultraviolet (UV) irradiation on the formation of a population of radiation-resistant melanoma cells; to study the features of cell signaling and the sensitivity of UV-resistant melanoma cells to the antitumor drugs.Materials and methods. The experiments were carried out on in vitro cultured A375 melanoma cells. Cells were cultured in a standard DMEM + 10 % FBS medium; cell growth rate was analyzed using the MTT assay; cell survival after irradiation was analyzed using a colony-forming test. Determination of the transcriptional activity of the estrogen receptor (ER) was performed by reporter analysis upon transfection into cells of a plasmid containing the luciferase reporter gene controlled by estrogen responsive element. The immunoblotting method was used to analyze the expression of cellular proteins; comparative analysis of ERα and ERβ expression was performed by immunofluorescent method.Results. Long-term UV irradiation leads to the formation of a UV-resistant subpopulation of A375 melanoma cells, which is characterized by decreased sensitivity to targeted (vemurafenib) and hormonal (tamoxifen) drugs, increased expression of Snail, an activator of the epithelial-mesenchymal transition, and in the absence of noticeable changes in the expression of PI3K / mTOR signaling. Metformin reduces Snail expression in both parental and UV-resistant A375 cells and enhances the cytostatic effect in combination with vemurafenib or tamoxifen.Conclusion. The data obtained demonstrate a decrease in the sensitivity of melanoma cells to targeted drugs under the long-term exposure to UV. The ability of metformin to potentiate the action of targeted drugs and inhibit Snail allows us to consider metformin not only as an antitumor agent, but also as a potential inhibitor of the epithelial-mesenchymal transition.
Mechanisms of cytotoxic activity of pyrrole-carboxamides against multidrug-resistant tumor cell sublines
Authors: Galembikova A. R. , Dunaev P. D. , Bikinieva F. F. , Mustafin I. G. , Kopnin P. B. , Zykova S. S. , Mukhutdinova F. I. , Sarbazyan E. A. , Boichuk S. V. 
Published: October 2023

Introduction. Mitotic poisoning agents (MPAs) affecting the dynamic state of the microtubules, are the well-known and effective chemotherapeutic agents. Mitotic poisoning agents are binding to the microtubules, and thereby interfere with tubulin polymerization or depolymerization dynamic state, resulting in the cell cycle arrest in M-phase (mitotic catastrophe) and subsequent apoptotic cell death. We reported previously about potent cytotoxic activities against the pyrrole-carboxamides (PCs) (PC-61 and PC-84) against broad spectrum of cancer cell lines, including triple negative breast cancer, lung and prostate cancer.Aim. To examine the cytotoxic activities of PC-61 and PC-84 against multidrug-resistant cancer cell lines indicated above.Materials and methods. Studу was performed on the triple-negative paclitaxel-resistant breast cancer cell line HCC1806 Tx-R and doxorubicin-resistant osteosarcoma SaOS-2 Dox-R cell line.Results. The cytotoxic activity of PCs was due to the inhibition of tubulin polymerization. Immunofluorescence staining data revealed PC’s ability to interfere with tubulin’s assembly in multidrug-resistant cancer cell lines. As an outcome of inhibition of tubulin polymerization, PCs induced cell cycle arrest in M-phase, and further led to apoptotic cell death of cancer cells.Conclusion. Collectively, we demonstrated potent cytotoxic activity of PCs against cancer cell lines with multidrug-resistant phenotype, which arising the possibilities to develop novel and effective anti-tumor agents that belongs to mitotic poisoning agents
Treatment options for dogs diagnosed with sinonasal aspergillosis
Authors: Belton Hannah , Lobb Jessica 
Published: October 2023

PICO question
In dogs diagnosed with SNA, does inclusion of debridement in the treatment protocol improve clinical outcomes compared to dogs treated without debridement?

Clinical bottom line
Category of research question
Treatment

The number and type of study designs reviewed
No papers that directly addressed the PICO were reviewed. Four retrospective case series which partially addressed the PICO question were discussed in the appraisal.

Strength of evidence
Zero.

Outcomes reported
None.

Conclusion
No conclusions can be made based on the current level of evidence, however the studies discussed in the appraisal suggest that there may be an association between the inclusion of debridement in the treatment protocol for SNA and an improved clinical outcome. Undertaking further higher-level studies (comparative, blind, prospective, randomised) would be required to confirm this. Ethical implications would not preclude such studies, for there is evidence that treatment protocols including topical deposition of antifungal agents alone are efficacious.

How to apply this evidence in practice
The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources.
Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.
Mathematic modeling of farmer household income in Singosari Village, Tanggamus District
Authors: Ariyanto Komang 
Published: October 2023

Farmers engage in various sectors (agriculture and non-agriculture) to earn income in order to meet their diverse household needs. Based on various pieces of literature, the agricultural sector serves as a gauge for the income level of rural farming households and provides a greater contribution than the non-agricultural sector. The purpose of this research is to analyze the mathematical modeling of household income among farmer households and to assess the contribution of the agricultural sector to households in Singosari Village, Tanggamus Regency. This study employs a quantitative method with a case study approach. The population in this study consisted of 464 heads of households, from which a sample of 30 households was selected. The respondents in this study were farmer households, and data were collected through surveys using questionnaires, documentation, and literature studies related to the mathematical modeling of income and the contribution of the agricultural sector to people's livelihoods. Data processing was conducted using SPSS software version 26, assisted by Microsoft Excel, and data analysis was performed using a simple linear regression model. The findings of this study indicate that, in Singosari Village, the agricultural income variable significantly influences the household income variable, as determined from the significance value of the simple linear regression results.Keywords: Agricultural income, household income, mathematical modeling, simple linear regression, singosari farmersMSC2020: 62P25
The development of a wellness tourism program in Thailand: a community-based participatory action research approach with an empowerment theoretical framework
Authors: Thojampa Somsak , Klankhajhon Sirikanok , Kumpeera Kittisak , Mongkholkham Chuthathip , Ellen Mawn Barbara 
Published: October 2023

Introduction: Thailand has not had a comprehensive effort to collaborate on a wellness tourism regional program for foreign travelers. The purpose of the study was exploring the key components necessary for a wellness tourism program in the lower northern region in Thailand.
Methods: This is a qualitative, community-based, participatory action research guided by the empowerment theory. A descriptive qualitative approach to data collection and analysis was used to explore stakeholders’ perspectives on the development of a wellness tourism program. A total of 40 key stakeholders from nine provinces in the lower north region of Thailand participated in two phases of the study’s data collection. Individual and focus groups interviews were conducted and analyzed thematically. The purposive sample included semi-structured questionnaires among community leaders, village health volunteers, and workers from various occupations who lived in the communities.
Results: Four themes emerged from the qualitative interviews and focus groups: 1) honoring local wisdom as the heart of a regional wellness tourism program; 2) the integration of historical traditions and regional lifestyles into a wellness tourism program; 3) the need for government supportive policies that help to fund community wellness initiatives to boost the local economy and tourism ;and 4) attention needed to improve the quality of life for the local communities while promoting wellness tourism in the region.
Conclusion: This community-based participatory action research approach created a synergy among stakeholders from multiple provinces in the lower north region of Thailand.
Rancang Bangun Website Reservasi Ramadhan Futsal Cilacap Dengan Metode Rapid Application Development
Authors: Wisnu Wardana Mohammad Adiyudha , Saputra Wahyu Andi 
Published: October 2023

Futsal is a sport that is also popular with soccer players or fans. This sport aims to score as many goals as possible into the opponent's goal, just like soccer. The futsal field which is smaller than soccer allows it to be played indoors or outdoors, in this case several enterprise provide futsal field rental services, one of which is Ramadhan Futsal located in Cilacap Regency, Central Java. The Ramadhan Futsal field reservation process still uses conventional methods by coming directly to the field or through whatsapp media, the payment process is also by coming directly to the location. The reservation process requires extra time and costs in traveling to the location. Based on these problems, this research proposes a solution in the form of a futsal field reservation web design. The development method used is Rapid Application Development with iteration in design step. The implementation process with PHP language assisted with Laravel framework and SQL database. Testing is conducted using the black-box testing method to Ramadhan Futsal worker and Visitor, from 20 stages of testing, all of the tests were declared as success.
EL VIDRIO DE VENTANA Y SU USO EN LA ARQUITECTURA ROMANA. A PROPÓSITO DE LOS VIDRIOS PLANOS DE LA VILLA DE RONDA DEL MARRUBIAL, CÓRDOBA / WINDOW GLASS AND ITS USE IN ROMAN ARCHITECTURE. REGARDING FLAT GLASSES DOCUMENTED IN THE VILLA OF RONDA DEL MARRUBIAL, CORDOBA
Authors: Velo Gala Almudena 
Published: October 2023

(ES)Resumen: La destacada ausencia de estudios referentes al vidrio y su aplicación como cierre de vanos en la arquitectura romana de la Bética, fue la principal motivación para proceder al estudio de un relevante conjunto de fragmentos de vidrio plano que se ocumentaron en el transcurso de las excavaciones de la conocida como villa de Ronda del Marrubial (Córdoba, España). Con este ejemplo culmina un trabajo que pretende ser, además, una revisión de las investigaciones existentes sobre el vidrio romano de ventana, sus técnicas de elaboración y funcionalidad en aquellos espacios a los que se haya asociado.Palabras clave: Vidrio romano; vidrio de ventana; Bética; Córdoba; Ronda del Marrubial. (EN)Abstract: There is an important absence related to glass and its uses in roman architecture in the Hispania Baetica. For this reason the study of an important group of flat glass fragments documented during excavation work at a villa, known as villa of Ronda del Marrubial (Cordoba, Spain), is particularly significant. This example is the end of a work which pretends to be a review about roman window glass researching, its manufacture and its association in Roman architectures.Keywords: Roman glass; window glass; Hispania Baetica; Cordoba; Ronda del Marrubial.
ARQUITECTURA DEFENSIVA ALMOHADE EN LA PROVINCIA DE CÓRDOBA. LOS EJEMPLOS DE HORNACHUELOS Y PALMA DEL RÍO / ALMOHAD DEFENSIVE ARCHITECTURE IN THE PROVINCE OF CORDOBA. EXAMPLES OF HORNACHUELOS AND PALMA DEL RÍO
Authors: Pabón Fernández Mª Piedad 
Published: October 2023

(ES)Resumen: Síntesis de nuestro Trabajo Fin de Master abordado con la idea de continuar la investigación arqueológica e histórica de la arquitectura defensiva en el Valle Medio del Guadalquivir durante el periodo almohade, contribuyendo a la elaboración de un catálogo de fortificaciones en este espacio y a su adecuada adscripción cronotipológica para entender el proceso histórico desde una base científica. Tras el análisis de los contextos geográfico e histórico en los que se insertan estas fortificaciones y del estado de la cuestión de la arquitectura defensiva almohade en al‐Andalus procedemos a analizar sus restos emergentes para poder confirmar, o no, la presencia de posibles fases constructivas almohades.Palabras clave: arqueología, al‐Andalus, fortificaciones, almohade, Valle del Guadalquivir(EN)Abstract: Synthesis about our End Master tackled with the idea of continuing the research of the archeological and historical investigation about the defensive architecture on the Guadalquivir’s Middle Valley during the almohad time, contributing to the development of a catalog of fortifications on this area and to a correct chronotypologist adscription to understand the historical process from a scientific base. After the analysis of the geographical and historical contexts where these fortifications are inserted and the question about the almohad defensive architecture in al‐Andalus, we are going to analise its emerging remains to confirm, or not, the presence of possible almohad constructive phases.Keywords: archeology, al‐Andalus, fortifications, almohad, Guadalquivir Valley
Hot Briquetting Technology for Ferrous Metal Waste in Molds with Movable Matrix
Authors: Litvinko A. A. 
Published: October 2023

The paper describes the technology of hot briquetting of structurally heterogeneous metalworking waste in molds with a movable matrix, which differs from known analogues in the possibility of improving the quality of briquettes with a minimum number of preparatory operations. The aim of the work is to improve the process of hot briquetting. The tasks to be solved are to develop a technological scheme and a physical and mathematical model of the process, reduce the forces of contact friction and tool wear, and optimize the energy-power parameters according to the criteria for the density and strength of briquettes. The technological scheme of briquetting is represented by a mold with a movable matrix, in which compressed air is used as an elastic support. During the briquetting process, the matrix and the pressed metal move in the same direction. The lateral friction force is active, directed towards the briquetting force. As a result, the force and work of briquetting, mold wear are reduced, and the briquette itself is of high quality – uniform in density and without internal defects.As a result of the numerical calculation of the model, the dependences of the stress state of the pressing and pressing pressure on the coordinates and density on the contact surfaces and in the depth of the material were obtained, which makes it possible to calculate the strength of the mold parts and select equipment for the briquetting process. The regularities of formation of the briquette structure are studied, the advantages of using the proposed technological scheme in comparison with briquetting in a fixed matrix are shown. The proposed technology eliminates the implementation of the preliminary operation of coolant removal by centrifugation or other known methods. Coolant is removed by thermal sublimation, partially burned in the furnace firebox, partially condensed in the “wet” cleaning system.
Survey Application Using GNSS F9R and WebGIS
Authors: Cahyadi Mokhamad Nur , Handayani Hepi Hapsari , Raharjo Agus Budi , Nurmianto Eko , Susanto Leviana Orchid Fiskarini , Haq Failaqul , Ramadhania Nurya , Triawan Luki Adi , Lest Dwi Sri 
Published: October 2023

Abstract

The expanding population in Surabaya, Indonesia, motivates the local water supply entity, Surya Sembada Surabaya's Local Water Supply Utility, to uphold the expected level of service concerning asset management. PDAM already employs a smartphone-based Global Navigation System (GNSS) for recording meter positions and water usage to manage their asset inventory, albeit with suboptimal position accuracy. The utilization of Geodetic GNSS is seen as less effective due to its considerable size and associated high expenses.
Effect of fertilization and container size on the quality of “Sangre de Drago” (Croton urucurana Baill.) seedlings in a plug +1 production system.
Authors: Martin Mariana , Castro Damian César , Gabriel Paola Marisel , Micheloud Norma Guadalupe , Álvarez Norma Hortensia , Buyatti Marcela Alejandra 
Published: October 2023

Abstract
“Sangre de Drago” (Croton urucurana Baill.) is a native tree of South America with high value for urban afforestation or the restoration of riparian forests. This study aimed to evaluate the effect of container size and fertilization on the quality of seedlings produced in a plug+1 system. In the establishment phase, the impact of container size (110 mL or 250 mL) and fertilization (with or without) on seedling quality were evaluated before transplanting into bigger containers. In the rapid growth phase, the effect of container size (1 L, 1.5 L, and 3.5 L) on survival and seedling quality was evaluated. At the end of each phase, seedling quality was evaluated through morphological and physiological attributes. Data were analyzed through linear models, generalized linear models, and nonlinear regressions. In the establishment phase, seedlings grown in 250 mL containers with fertilization showed the best performance in morphological and physiological variables and the Dickson quality index. In the rapid growth phase, seedlings grown in 3.5 L containers showed the highest values in total height, stem diameter, total dry weight, Dickson quality index, and the lowest mortality at the end of the experiment. The results of this study suggest that the most efficient strategy for producing high-quality "Sangre de drago" tree seedlings is to use 250 mL tubes during the establishment phase, followed by transplanting them into 3.5 L pots during the rapid growth phase.