bluuebunny/crossref_metadata_2025_split huggingface dataset:
AS602801 treatment suppresses breast cancer metastasis to the brain by interfering with gap‐junction communication by regulating Cx43 expression
Authors: Yang Zhigang , Yang Liguo , Zhang Jun , Qian Chenzeyue , Zhao Yi 
Published: October 2023

AbstractAS602801 has been reported as a potential drug candidate against brain metastasis by suppressing the gap‐junction communication between lung cancer stem cells and astrocytes. In this study, we aimed to study the molecular mechanism underlying the role of AS602801 in the treatment of brain metastasis in breast cancer. We utilized female athymic BALB/c nude mice and MDA‐MB‐231/BT‐474BR cells to establish experimental models. Polymerase chain reaction assays were performed to observe changes in the connexin 43 (Cx43) messenger RNA (mRNA) and c‐Jun N‐terminal kinase (JNK) mRNA levels. Dye transfer assay was used to observe the effect of AS602801 on cell–cell communication. An organotypic blood–brain barrier (BBB) model was utilized to observe the effect of AS602801 on transmigration through the BBB barrier. MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide) assay and flow cytometry were performed to evaluate the proliferation and apoptosis of breast cancer cells co‐cultivated with astrocytes. AS602801 inhibited the upregulation of Cx43 and JNK in brain metastasized breast cancer cells in a dose‐dependent manner. Also, AS602801 significantly decreased the dye transfer rate from astrocytes to breast cancer cells, indicating the inhibitory effect of AS602801 on cell–cell communication. The transmigration ability of breast cancer cells co‐cultured with astrocytes was decreased by AS602801. Furthermore, AS602801 reduced the elevated Cx43/JNK mRNA expression in the co‐astrocyte group while suppressing the increased proliferation and promoting the decreased apoptosis of breast cancer cells co‐cultivated with astrocytes. AS602801 also suppressed the brain metastasis of breast cancer cells and increased mouse survival. AS602801 downregulates the expressions of JNK and Cx43 to suppress the gap‐junction activity. AS602801 also inhibits the communication between breast cancer cells and astrocytes, thus contributing to the treatment of brain metastasis in breast cancer.
Trisiloxane Surfactants Negatively Affect Reproductive Behaviors and Enhance Viral Replication in Honey Bees
Authors: Fine Julia D. , Cox-Foster Diana L. , Moor Kyle J. , Chen Ruiwen , Avalos Arian 
Published: October 2023

Abstract
Trisiloxane surfactants are often applied in formulated adjuvant products to blooming crops, including almonds, exposing the managed honey bees (Apis mellifera) used for pollination of these crops and persisting in colony matrices, such as bee bread. Despite this, little is known regarding the effects of trisiloxane surfactants on important aspects of colony health, such as reproduction. In the present study, we use laboratory assays to examine how exposure to field-relevant concentrations of three trisiloxane surfactants found in commonly used adjuvant formulations affect queen oviposition rates, worker interactions with the queen, and worker susceptibility to endogenous viral pathogens. Trisiloxane surfactants were administered at 5 mg/kg in pollen supplement diet for 14 days. No effects on worker behavior or physiology could be detected, but our results demonstrate that hydroxy-capped trisiloxane surfactants can negatively affect queen oviposition and methyl-capped trisiloxane surfactants cause increased replication of Deformed Wing Virus in workers, suggesting that trisiloxane surfactant use while honey bees are foraging may negatively impact colony longevity and growth. Environ Toxicol Chem 2024;43:222–233. © 2023 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Toxicant Responses and Culturing Characteristics of Long-Term Laboratory-Reared and Field Populations of Ceriodaphnia dubia
Authors: Lydy Victoria R. , Regn Orithea Z. , Bouldin Jennifer L. 
Published: October 2023

Abstract
Ceriodaphnia dubia is a standardized test organism for regulatory toxicity testing of surface waters and commercial chemicals because of its simplicity to culture and responsiveness to toxicants. For testing convenience, C. dubia is often cultured for extended periods in the laboratory with little knowledge of the impact on subsequent generations. Extended laboratory rearing could impact how they respond to stressors and decrease the accuracy of test results. The present study investigated if C. dubia cultured for an extended period were representative of three recently collected field populations by comparing their culturing characteristics and sensitivities to toxicants. For culturing characteristics, the field cultures were more challenging because they had shorter body lengths, fewer neonates, and higher mortality rates than the laboratory culture. Comparative chronic toxicity tests with sodium chloride and the neonicotinoid insecticide thiamethoxam indicated that the laboratory and field organisms did not differ much in their toxicological responses but did differ in the variability of responses (percentage of coefficient of variation). The differences between the laboratory and field cultures found in the present study highlight the challenges of addressing discrepancies between laboratory and field applications in existing standardized methodologies. Environ Toxicol Chem 2024;43:159–169. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Determinants of climate information service access and use among smallholder farmers in Bereh woreda, Ethiopia
Authors: Fikadu Tsion , Damene Shimeles , Teklu Abyiot 
Published: October 2023

Abstract
Climate information service (CIS) is a key component of a climate adaptation strategy that is expected to lessen climate risk. Access to and use of CIS among the local community are limited and constrained by various factors and are not supported by empirical research evidence. Therefore, this article analyzed CIS access and use determinants among smallholder farmers in Bereh woreda. The study applied a cross-sectional design with a mixed methodological approach. Data were collected through a survey of 219 smallholder farmer households, three focused-group discussions, and six key informant interviews. The collected survey data were analyzed using the heckprobit model to determine factors influencing smallholder farmers' access to and use of CIS. The heckprobit model results revealed that the determinants of CIS access and use had a statistical significance of log-likelihood of 1% (Wald χ2 = 45.2, p = 0.001), indicating a strong explanatory power. The selection model revealed that age and off-farm income significantly reduced the likelihood of accessing CIS, whereas mobile-phone ownership and male-headed households increased the likelihood of accessing it. Age, female-headed households, and farm size decreased the likelihood of using CIS, whereas mixed farming, radio ownership, and access to herbicide enhanced the likelihood of using it. This study, therefore, recommends intensive awareness creation and improving the delivery of diverse and reliable CIS to enhance agricultural productivity and smallholder farmers' resilience to the impacts of climate change. Integr Environ Assess Manag 2024;20:794–804. © 2023 SETAC
Plasma cell‐free DNA methylation analysis for ovarian cancer detection: Analysis of samples from a case‐control study and an ovarian cancer screening trial
Authors: Herzog Chiara , Jones Allison , Evans Iona , Reisel Daniel , Olaitan Adeola , Doufekas Konstantinos , MacDonald Nicola , Rådestad Angelique Flöter , Gemzell‐Danielsson Kristina , Zikan Michal , Cibula David , Dostálek Lukáš , Paprotka Tobias , Leimbach Andreas , Schmitt Markus , Ryan Andy , Gentry‐Maharaj Aleksandra , Apostolidou Sophia , Rosenthal Adam N , Menon Usha , Widschwendter Martin 
Published: October 2023

AbstractAnalysis of cell‐free DNA methylation (cfDNAme), alone or combined with CA125, could help to detect ovarian cancers earlier and may reduce mortality. We assessed cfDNAme in regions of ZNF154, C2CD4D and WNT6 via targeted bisulfite sequencing in diagnostic and early detection (preceding diagnosis) settings. Diagnostic samples were obtained via prospective blood collection in cell‐free DNA tubes in a convenience series of patients with a pelvic mass. Early detection samples were matched case‐control samples derived from the UK Familial Ovarian Cancer Screening Study (UKFOCSS). In the diagnostic set (ncases = 27, ncontrols = 41), the specificity of cfDNAme was 97.6% (95% CI: 87.1%‐99.9%). High‐risk cancers were detected with a sensitivity of 80% (56.3%‐94.3%). Combination of cfDNAme and CA125 resulted in a sensitivity of 94.4% (72.7%‐99.9%) for high‐risk cancers. Despite technical issues in the early detection set (ncases = 29, ncontrols = 29), the specificity of cfDNAme was 100% (88.1%‐100.0%). We detected 27.3% (6.0%‐61.0%) of high‐risk cases with relatively lower genomic DNA (gDNA) contamination. The sensitivity rose to 33.3% (7.5%‐70.1%) in samples taken <1 year before diagnosis. We detected ovarian cancer in several patients up to 1 year before diagnosis despite technical limitations associated with archival samples (UKFOCSS). Combined cfDNAme and CA125 assessment may improve ovarian cancer screening in high‐risk populations, but future large‐scale prospective studies will be required to validate current findings.
Deep dynamic face capture and recognition based on internet of things
Authors: Ai Cunchi , Lin Tianqi , Shi Wandi 
Published: October 2023

AbstractFace recognition is widely used as identity authentication method in the Internet of Things (IoTs). However, compared with static camera, the background of IoT devices may change. Additionally, the facial expression or posture may change as well. Although face recognition has been researched for several decades, it is still a challenging task to design a robust face recognition system in IoT environment. This paper designs a special face recognition system by introducing deep transfer learning to adapt difference scenes in IoT environment. The face recognition system consists of two parts: terminal smart devices and cloud center. The terminal smart device consists of a camera to capture face picture, a wireless network part to communicate with cloud center, and a screen to show recognition result. The cloud center receives the face picture from terminal smart device and returns the face recognition result by using an offline deep transfer learning model. Compared with previous models, deep transfer learning model can better adapt different scenes. The experiments shows that the proposed face recognition system achieves more promising results than two source‐only models and two shallow transfer learning models.
Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds
Authors: Dewey Marley J. , Timmer Kyle B. , Blystone Ashley , Lu Crislyn , Harley Brendan A. C. 
Published: October 2023

AbstractCurrent treatments for craniomaxillofacial (CMF) defects motivate the design of instructive biomaterials that can promote osteogenic healing of complex bone defects. We report methods to promote in vitro osteogenesis of human mesenchymal stem cells (hMSCs) within a model mineralized collagen scaffold via the incorporation of ascorbic acid (vitamin C), a key factor in collagen biosynthesis and bone mineralization. An addition of 5 w/v% ascorbic acid into the base mineralized collagen scaffold significantly changes key morphology characteristics including porosity, macrostructure, and microstructure. This modification promotes hMSC metabolic activity, ALP activity, and hMSC‐mediated deposition of calcium and phosphorous. Additionally, the incorporation of ascorbic acid influences osteogenic gene expression (BMP‐2, RUNX2, COL1A2) and delays the expression of genes associated with osteoclast activity and bone resorption (OPN, CTSK), though it reduces the secretion of OPG. Together, these findings highlight ascorbic acid as a relevant component for mineralized collagen scaffold design to promote osteogenic differentiation and new bone formation for improved CMF outcomes.
Palovarotene Action Against Heterotopic Ossification Includes a Reduction of Local Participating Activin A‐Expressing Cell Populations
Authors: Mundy Christina , Yao Lutian , Shaughnessy Kelly A. , Saunders Cheri , Shore Eileen M. , Koyama Eiki , Pacifici Maurizio 
Published: October 2023

ABSTRACTHeterotopic ossification (HO) consists of extraskeletal bone formation. One form of HO is acquired and instigated by traumas or surgery, and another form is genetic and characterizes fibrodysplasia ossificans progressiva (FOP). Recently, we and others showed that activin A promotes both acquired and genetic HO, and in previous studies we found that the retinoid agonist palovarotene inhibits both HO forms in mice. Here, we asked whether palovarotene's action against HO may include an interference with endogenous activin A expression and/or function. Using a standard mouse model of acquired HO, we found that activin A and its encoding RNA (Inhba) were prominent in chondrogenic cells within developing HO masses in untreated mice. Single‐cell RNAseq (scRNAseq) assays verified that Inhba expression characterized chondroprogenitors and chondrocytes in untreated HO, in addition to its expected expression in inflammatory cells and macrophages. Palovarotene administration (4 mg/kg/d/gavage) caused a sharp inhibition of both HO and amounts of activin A and Inhba transcripts. Bioinformatic analyses of scRNAseq data sets indicated that the drug had reduced interactions and cross‐talk among local cell populations. To determine if palovarotene inhibited Inhba expression directly, we assayed primary chondrocyte cultures. Drug treatment inhibited their cartilaginous phenotype but not Inhba expression. Our data reveal that palovarotene markedly reduces the number of local Inhba‐expressing HO‐forming cell populations. The data broaden the spectrum of HO culprits against which palovarotene acts, accounting for its therapeutic effectiveness. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Classification of Fracture Risk in Fallers Using Dual‐Energy X‐Ray Absorptiometry (DXA) Images and Deep Learning‐Based Feature Extraction
Authors: Senanayake Damith , Seneviratne Sachith , Imani Mahdi , Harijanto Christel , Sales Myrla , Lee Peter , Duque Gustavo , Ackland David C. 
Published: October 2023

AbstractDual‐energy X‐ray absorptiometry (DXA) scans are one of the most frequently used imaging techniques for calculating bone mineral density, yet calculating fracture risk using DXA image features is rarely performed. The objective of this study was to combine deep neural networks, together with DXA images and patient clinical information, to evaluate fracture risk in a cohort of adults with at least one known fall and age‐matched healthy controls. DXA images of the entire body as, well as isolated images of the hip, forearm, and spine (1488 total), were obtained from 478 fallers and 48 non‐faller controls. A modeling pipeline was developed for fracture risk prediction using the DXA images and clinical data. First, self‐supervised pretraining of feature extractors was performed using a small vision transformer (ViT‐S) and a convolutional neural network model (VGG‐16 and Resnet‐50). After pretraining, the feature extractors were then paired with a multilayer perceptron model, which was used for fracture risk classification. Classification was achieved with an average area under the receiver‐operating characteristic curve (AUROC) score of 74.3%. This study demonstrates ViT‐S as a promising neural network technique for fracture risk classification using DXA scans. The findings have future application as a fracture risk screening tool for older adults at risk of falls. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Scanning electron microscopy of digestive tract parasites in jungle cat (Felis chaus)
Authors: Hashemnia Shadi , Khovand Hosein , Ghahvei Younes , Nourollahifard Saeidreza 
Published: October 2023

AbstractAmong the 10 native cats of Iran, the Felis chaus is considered as one of the largely unknown species. Despite the reports on jungle cats in southeast Iran, there is no comprehensive information about their parasitic diseases. Scanning electron microscope (SEM) provides a valuable tool for the study of these organisms. In October 2022, a 15‐year‐old male jungle cat (F. chaus) was examined for gastrointestinal parasites using SEM which revealed exact parasite structures such as the mouth, shape of the lips, teeth and their position, papillae, excretory pore, and amphids. SEM of the Taenia taeniaeformis found in the F. chaus intestinal epithelium revealed that this tapeworm consisted of four unarmed suckers and a large armed rostellum. The rostellum was observed bearing double rings of alternating and circular hook arrangements. There were 21 large hooks in the anterior ring and 21 slightly smaller hooks in the posterior one. This study is the first report of Joyeuxiella echinorhyncoides from a jungle cat (F. chaus) in southeastern Iran. The rostellum was cylindrical and armed with thorn shape hooks that covered the whole rostellum with similar density. It was armed with 20–30 long hooks arranged in 16 rows. Four big suckers were visible at the base of the rostellum, two on each side. The suckers were round, and unlike the rostellum, these structures were unarmed. The results of the present study observed under SEM, indicated that organs such as sucker and rostellum in cestodes and mouth and the shape of lips and teeth in nematodes played a significant role in the biology of these helminths. The SEM reveals the surface structures and their relationships which results in better distinguishing the species and showing their detailed differences. More SEM studies need to be conducted on other species of cestodes and nematodes, along with pathological tests, to obtain more comprehensive and precise information about how these parasites harm this jungle cat host. The results of present study indicate that morphological details are important which should be considered in the classification. Meanwhile, external structures described herein using SEM, further investigation using transmission electron microscope provide more data on the biology of the parasites.Research Highlights
Light microscopy is the first step to identify the parasites.
High magnification 3D images of the SEM reveal the surface structures of parasites and their relationships precisely.
SEM study of the parasites results in distinguishing the species and showing their detailed differences.
Effect of cadmium on the regulatory mechanism of steroidogenic pathway of Leydig cells during spermatogenesis
Authors: Ji HengLi , Fan Wei , Kakar Mohibullah , Alajmi Reem Atalla , Bashir Muhammad Amjad , Shakir Yasmeen 
Published: October 2023

AbstractCadmium is a male reproductive toxicant that interacts with a variety of pathogenetic mechanisms. However, the effect of cadmium on the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis is still ambiguous. Light microscopy, Western blot, immunohistochemistry, immunofluorescence, and quantitative polymerase chain reaction were performed to study the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis. The results indicated that in the control group, Leydig cells showed dynamic immunoreactivity and immunosignaling action with a strong positive significant secretion of 3β‐hydroxysteroid hydrogenase (3β‐HSD) in the interstitial compartment of the testis. Leydig cells showed a high active regulator mechanism of the steroidogenic pathway with increased the proteins and genes expression level of steroidogenic acute regulatory protein (STAR), cytochrome P450 cholesterol (CYP11A1), cytochrome P450 cholesterol (CYP17A1), 3β‐hydroxysteroid hydrogenase (3β‐HSD) 17β‐hydroxysteroid hydrogenase (17β‐HSD), and androgen receptor (AR) that maintained the healthy and vigorous progressive motile spermatozoa. However, on treatment with cadmium, Leydig cells were irregularly dispersed in the interstitial compartment of the testis. Leydig cells showed reduced immunoreactivity and immunosignaling of 3β‐HSD protein. Meanwhile, cadmium impaired the regulatory mechanism of the steroidogenic process of the Leydig cells with reduced protein and gene expression levels of STAR, CYP11A1, CYP17A1, 3β‐HSD, 17β‐HSD, and AR in the testis. Additionally, treatment with cadmium impaired the serum LH, FSH, and testosterone levels in blood as compared to control. This study explores the hazardous effect of cadmium on the regulatory mechanism of the steroidogenic pathway of Leydig cells during spermatogenesis.
Anger for good? Unethical‐behavior‐targeted leader anger expression and its consequences on team outcomes
Authors: Zhang Shimin , Mo Shenjiang , Liu Wu 
Published: October 2023

SummaryAlthough leader anger expression targeted at employees' unethical behavior is pervasive in the workplace, we still know little about its theoretical meaning and consequences. To address this theoretical blind spot, we drew on fairness heuristic theory to investigate whether, how, and when unethical‐behavior‐targeted (UB‐targeted) leader anger expression affects team outcomes. Our findings from two time‐lagged field studies suggest that a punishment‐based distributive justice climate mediates the positive effects of UB‐targeted leader anger expression on team organizational citizenship behavior (OCB) and team viability. Moreover, leader moral decoupling weakens these indirect relationships. Specifically, the indirect relationships are weaker when the leader separated judgments of performance from those of ethics. These findings highlight the importance of a fairness perspective in understanding the consequences of leader anger expression targeted at unethical behaviors.
Layered structures of robustness and resilience: Evidence from cybersecurity projects for critical infrastructures in Central Europe
Authors: Maurer Florian , Fritzsche Albrecht 
Published: October 2023

AbstractThe article studies cybersecurity projects from the perspective of social systems theory. Measures taken to increase cybersecurity are aimed at the robustness and resilience of specific systemic entities in society. We argue that this does not necessarily mean that systems in which these entities are embedded become more robust or resilient. The article investigates the objectives of 20 projects funded by different public organizations to increase cybersecurity of critical infrastructures. The findings show that robustness and resilience are addressed by these projects on different layers of systems in society, without a clear picture of how they are supposed to fit together in a wider effort to protect society against disruption. The detailed analysis of the different facets of robustness and resilience addressed in cybersecurity projects paves the way for a better understanding of this problem. The research shows how organizations may avoid the negative consequences of measures to secure specific systemic entities in society in the case of critical infrastructures, other application scenarios, and digital innovation in general.