bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pre-operative clonal hematopoiesis is related to adverse outcome in lung cancer after adjuvant therapy
Authors: Yun Jae Kwang , Kim Sugyeong , An Hongyul , Lee Geun Dong , Kim Hyeong Ryul , Kim Yong-Hee , Kim Dong Kwan , Park Seung-Il , Choi Sehoon , Koh Youngil 
Published: December 2023

Abstract
Background
Clonal hematopoiesis (CH) frequently progresses after chemotherapy or radiotherapy. We evaluated the clinical impact of preoperative CH on the survival outcomes of patients with non-small cell lung cancer (NSCLC) who underwent surgical resection followed by adjuvant therapy.

Methods
A total of 415 consecutive patients with NSCLC who underwent surgery followed by adjuvant therapy from 2011 to 2017 were analyzed. CH status was evaluated using targeted deep sequencing of blood samples collected before surgery. To minimize the possible selection bias between the two groups according to CH status, a propensity score matching (PSM) was adopted. Early-stage patients were further analyzed with additional matched cohort of patients who did not receive adjuvant therapy.

Results
CH was detected in 21% (86/415) of patients with NSCLC before adjuvant therapy. Patients with CH mutations had worse overall survival (OS) than those without (hazard ratio [95% confidence interval] = 1.56 [1.07–2.28], p = 0.020), which remained significant after the multivariable analysis (1.58 [1.08–2.32], p = 0.019). Of note, the presence of CH was associated with non–cancer mortality (p = 0.042) and mortality of unknown origin (p = 0.018). In patients with stage IIB NSCLC, there was a significant interaction on OS between CH and adjuvant therapy after the adjustment with several cofactors through the multivariable analysis (HR 1.19, 95% CI 1.00–1.1.41, p = 0.041).

Conclusions
In resected NSCLC, existence of preoperative CH might amplify CH-related adverse outcomes through adjuvant treatments, resulting in poor survival results.
Effects of biofeedback on biomechanical factors associated with chronic ankle instability: a systematic review with meta-analysis
Authors: Mousavi Seyed Hamed , Khorramroo Fateme , Minoonejad Hooman , Zwerver Johannes 
Published: December 2023

Abstract
Background
Biofeedback may alter the biomechanics of lower extremities in patients with chronic ankle instability (CAI). We aimed to systematically review the literature on the effect of gait-training and biofeedback on biomechanical parameters in individuals with CAI and conduct a meta-analysis.

Methods
We searched four databases including PubMed, Web of Science, Scopus and Embase from their inception through 30th June 2022. The Downs and Black appraisal scale was applied to assess quality of included studies. Two reviewers screened studies to identify those reporting the effect of biofeedback on biomechanical factors associated with CAI. Outcomes of interest were kinetics and kinematics. Two authors separately extracted data from included studies. Data of interest were study design, number of sessions, intervention, tools, outcomes, number, sex, age, height, and body mass of participants.

Results
Thirteen studies with a total of 226 participants were included. Biofeedback was capable of shifting center of pressure (COP) and lateral plantar pressure medially and reducing foot inversion, adduction, propulsive vertical ground reaction force (vGRF), ankle joint contact force, peak pressure and pressure time integral in the lateral mid-foot and forefoot. Auditory biofeedback had agreater impact on modifying plantar pressure in individuals with CAI. The meta-analyses revealed that visual biofeedback reduces peak pressure in lateral mid-foot and pressure time integral at lateral and medial heel and pressure increases under the hallux.

Conclusion
Biofeedback can alter pressure, vGRF, and foot inversion associated with CAI. Auditory biofeedback had greater impact on modifying plantar pressure in individuals with CAI. Further studies are required to assess the prolonged effect and clinical consequences of biofeedback or a combination of feedback on CAI in different age groups. Moreover, developing a low-cost and user-friendly device that can be evaluated in high quality RCTs is important prior to implementing the intervention in the clinical setting to reduce symptoms of CAI.
Alterations in DNA methylation associate with reduced migraine and headache days after medication withdrawal treatment in chronic migraine patients: a longitudinal study
Authors: Mehta Divya , de Boer Irene , Sutherland Heidi G. , Pijpers Judith A. , Bron Charlene , Bainomugisa Charlotte , Haupt Larisa M. , van den Maagdenberg Arn M. J. M. , Griffiths Lyn R. , Nyholt Dale R. , Terwindt Gisela M. 
Published: December 2023

Abstract
Background
Chronic migraine, a highly disabling migraine subtype, affects nearly 2% of the general population. Understanding migraine chronification is vital for developing better treatment and prevention strategies. An important factor in the chronification of migraine is the overuse of acute headache medication. However, the mechanisms behind the transformation of episodic migraine to chronic migraine and vice versa have not yet been elucidated. We performed a longitudinal epigenome-wide association study to identify DNA methylation (DNAm) changes associated with treatment response in patients with chronic migraine and medication overuse as part of the Chronification and Reversibility of Migraine clinical trial. Blood was taken from patients with chronic migraine (n = 98) at baseline and after a 12-week medication withdrawal period. Treatment responders, patients with ≥ 50% reduction in monthly headache days (MHD), were compared with non-responders to identify DNAm changes associated with treatment response. Similarly, patients with ≥ 50% versus < 50% reduction in monthly migraine days (MMD) were compared.

Results
At the epigenome-wide significant level (p < 9.42 × 10–8), a longitudinal reduction in DNAm at an intronic CpG site (cg14377273) within the HDAC4 gene was associated with MHD response following the withdrawal of acute medication. HDAC4 is highly expressed in the brain, plays a major role in synaptic plasticity, and modulates the expression and release of several neuroinflammation markers which have been implicated in migraine pathophysiology. Investigating whether baseline DNAm associated with treatment response, we identified lower baseline DNAm at a CpG site (cg15205829) within MARK3 that was significantly associated with MMD response at 12 weeks.

Conclusions
Our findings of a longitudinal reduction in HDAC4 DNAm status associated with treatment response and baseline MARK3 DNAm status as an early biomarker for treatment response, provide support for a role of pathways related to chromatin structure and synaptic plasticity in headache chronification and introduce HDAC4 and MARK3 as novel therapeutic targets.
Lipidomics profiling reveals distinct patterns of plasma sphingolipid alterations in Alzheimer’s disease and vascular dementia
Authors: Chua Xin Ying , Torta Federico , Chong Joyce R. , Venketasubramanian Narayanaswamy , Hilal Saima , Wenk Markus R. , Chen Christopher P. , Arumugam Thiruma V. , Herr Deron R. , Lai Mitchell K. P. 
Published: December 2023

AbstractBackgroundAlzheimer’s disease (AD) and vascular dementia (VaD) are two of the commonest causes of dementia in the elderly. Of the myriad biomolecules implicated in dementia pathogenesis, sphingolipids have attracted relatively scant research attention despite their known involvement in multiple pathophysiological processes. The potential utility of peripheral sphingolipids as biomarkers in dementia cohorts with high concomitance of cerebrovascular diseases is also unclear.MethodsUsing a lipidomics platform, we performed a case–control study of plasma sphingolipids in a prospectively assessed cohort of 526 participants (non-cognitively impaired, NCI = 93, cognitively impaired = 217, AD = 166, VaD = 50) using a lipidomics platform.ResultsDistinct patterns of sphingolipid alterations were found in AD and VaD, namely an upregulation of d18:1 species in AD compared to downregulation of d16:1 species in VaD. In particular, GM3 d18:1/16:0 and GM3 d18:1/24:1 showed the strongest positive associations with AD. Furthermore, evaluation of sphingolipids panels showed specific combinations with higher sensitivity and specificity for classification of AD (Cer d16:1/24:0. Cer d18:1/16:0, GM3 d16:1/22:0, GM3 d18:1/16:0, SM d16:1/22:0, HexCer d18:1/18:0) and VAD (Cer d16:1/24:0, Cer d18:1/16:0, Hex2Cer d16:1/16:0, HexCer d18:1/18:0, SM d16:1/16:0, SM d16:1/20:0, SM d18:2/22:0) compared to NCI.ConclusionsAD and VaD are associated with distinct changes of plasma sphingolipids, warranting further studies into underlying pathophysiological mechanisms and assessments of their potential utility as dementia biomarkers and therapeutic targets.
Plasmodium ovale infection in Sri Lanka: distant exposure and incidental detection of hyperparasitemia: a case report
Authors: Kularatne Damsara , Chulasiri Pubudu , Dharmapala Arinda , Kularatne Senanayake 
Published: December 2023

Abstract
Background
Plasmodium ovale malaria, which was previously endemic to tropical Africa and the Southwest Pacific islands is now being reported from parts of Asia. In Sri Lanka, the indigenous transmission of malaria has not been documented since October 2012. Since then, there have been several imported cases of malaria, including P.ovale, which have been detected sporadically. The reporting case of P. ovale was imported and detected incidentally in 2021, with several atypical presentations.

Case presentation
A 40-year-old Sri Lankan medical doctor developed continuous fever with chills, rigors, and dysuria a day following removal of a large lipoma at the root of the neck under general anaesthesia. When the fever has been responding to antibiotics, on the 4th postoperative day a mild thrombocytopenia on complete blood count was detected. A blood smear which was done on the 5th postoperative day incidentally found a malaria parasite and confirmed as Plasmodium ovale with a density of 6535 parasites/microliter on the same day. He never had malaria in the past, but he had worked in South Sudan 1 year ago and visited India six months ago. On the 6th postoperative day, he was treated with chloroquine, and hyperparasitemia reduced rapidly by the next day. As the fever recurred with clinical deterioration, he was treated with different antibiotics. During the course of the illness, he did not develop pallor, or icterus except for a palpable soft spleen. The parasite count was zero on the 9th postoperative day and his fever subsided on the next day. Further, he was treated with primaquine to prevent future relapse and transmission.

Conclusion
A long incubation period, incidental detection of P ovale in a blood smear, and hyperparasitaemia are the atypical presentations of this case. Postoperative bacterial infection and stress may have reactivated the dormant malaria (hyponozoites) in this patient with an unusual picture. Coinfection of malaria with bacterial sepsis is a challenge in the management of the patient. As the Anopheles mosquito vector exists in Sri Lanka, the risk of indigenous transmission is high from such imported cases of P. ovale.
Streptococcus ruminantium-associated sheep mastitis outbreak detected in Italy is distinct from bovine isolates
Authors: Rosa Maria Nives , Vezina Ben , Marogna Gavino , Canu Antonella , Molotzu Monica Rosaria , Tola Sebastiana 
Published: December 2023

AbstractStreptococcus ruminantiumis the causative agent of several bovine and ovine diseases, however reports are uncommon and application of whole genome sequencing to identify is rare. We report for the first time, a severe ovine mastitis outbreak caused byS. ruminantiumin Italy, 2022.S. ruminantiumwas isolated from 12 adult lactating ewes with diffuse nodules in the mammary parenchyma and predominantly serous and clotted milk. All outbreak isolates, along with five additional historical Italian isolates (between 2011 and 2017), were genomically characterised and then analysed in the context of all publicly availableS. ruminantiumgenomes. Antimicrobial susceptibility testing was performed to determine the MICs of 16 antibiotics. The results showed that all isolates were susceptible to all antimicrobials tested except kanamycin. Single Nucleotide Variant analysis confirmed this as a clonal outbreak across 10 sheep (≤ 15 SNVs), while the two others were colonised by more distantly related clones (≤ 53 pairwise SNVs), indicating the presence of multiple infecting lineages. The five historicalS. ruminantiumisolates were comprised of genetically-distant singletons (between 1259 and 5430 pairwise SNVs to 2022 outbreak isolates). Ovine isolates were found to be genetically distinct to bovine isolates, forming monophyletic groups. Bovine isolates were similarly made up of singleton clones in all but two isolates. Taken together, our genomic analysis using all globally available genomes is consistent with general opportunistic pathogenesis ofS. ruminantium. We encourage future genomic surveillance efforts to facilitate outbreak detection, as well as improve our understanding of this poorly-understood, multi-host, zoonotic pathogen.
Existence theory and Ulam’s stabilities for switched coupled system of implicit impulsive fractional order Langevin equations
Authors: Rizwan Rizwan , Liu Fengxia , Zheng Zhiyong , Park Choonkil , Paokanta Siriluk 
Published: December 2023

AbstractIn this work, a system of nonlinear, switched, coupled, implicit, impulsive Langevin equations with two Hilfer fractional derivatives is introduced. The suitable conditions and results are established to discuss existence, uniqueness, and Ulam-type stability results of the mentioned model, with the help of nonlinear functional analysis techniques and Banach’s fixed-point theorem. Furthermore, we examine our results with the help of example.
Efficient and privacy-preserving image classification using homomorphic encryption and chunk-based convolutional neural network
Authors: Jia Huixue , Cai Daomeng , Yang Jie , Qian Weidong , Wang Cong , Li Xiaoyu , Yang Shan 
Published: December 2023

AbstractImage feature categorization has emerged as a crucial component in many domains, including computer vision, machine learning, and biometrics, in the dynamic environment of big data and cloud computing. It is extremely difficult to guarantee image data security, privacy, and computing efficiency while also lowering storage and transmission costs. This paper introduces a novel method for classifying image features that combines multilevel homomorphic encryption and image data partitioning in an integrated manner. We employ a novel partitioning strategy to reduce computational complexity, significantly reducing computational load and improving classification accuracy. In the quest for increased data security and privacy, we introduce a novel, fully homomorphic encryption approach specialized to partitioned images. To counter the inherent complexity of encryption, we devise a compound encryption strategy that exploits the full potential of homomorphic computation, with an explicit objective to curtail computational and storage overheads. Evidently superior to conventional methods, our methodology showcases pronounced benefits in computational efficiency, storage and transmission cost reduction, and robust security and privacy preservation. Hence, the methodology put forth in this paper presents a pioneering and efficacious resolution to the multifaceted challenges of image feature classification within the intricate milieu of cloud computing and big data.
Downscaling precipitation and temperature in the Andes: applied methods and performance—a systematic review protocol
Authors: Núñez Mejía Santiago , Villegas-Lituma Carina , Crespo Patricio , Córdova Mario , Gualán Ronald , Ochoa Johanna , Guzmán Pablo , Ballari Daniela , Chávez Alexis , Mendoza Paz Santiago , Willems Patrick , Ochoa-Sánchez Ana 
Published: December 2023

Abstract
Background
Global warming and climate change are threats to the world. Warmer temperatures and changes in precipitation patterns alter water availability and increase the occurrence of extreme weather events. South America and the Andes are vulnerable regions to climate change due to inequity and the uneven distribution of resources. Climate change evaluation often relies on the use of general circulation models (GCMs). However, the spatial resolution is too coarse and does not provide a realistic climate representation at a local level. This is of particular importance in mountain areas such as the Andes range, where the heterogeneous topography requires a finer spatial resolution to represent the local physical processes. To this end, statistical and/or dynamical downscaling methods are required. Several approaches and applications of downscaling procedures have been carried out in the countries of this region, with different purposes and performances. However, the main objective is to improve the representation of meteorological variables such as precipitation and temperature. A systematic review of these downscaling applications will identify the performance of the methods applied in the Andes region for the downscaling of precipitation and temperature. In addition, the meta-analysis could detect factors influencing the performance. The overall goal is to highlight promising methods in terms of fitness for use and identify knowledge gaps in the region.

Methods
The review will search and examine published and grey literature on downscaling applications of temperature and precipitation in the Andes region. Predetermined criteria for eligibility will allow the screening of the evidence. Then, the method used in each application will be coded and mapped according to the country, purpose, variable, and type of downscaling. At the same time, quantitative and qualitative data will be extracted. The performance metrics are particularly interesting for this review. A meta-analysis will be conducted for those studies with comparable metrics. A narrative synthesis, maps and heatmaps will show the results. Tables, funnel plots, and meta-regressions will present the meta-analysis. Throughout the review, a critical appraisal step will categorize the validity of the evidence.
Problematic mobile phone use and time management disposition in Chinese college students: the chain mediating role of sleep quality and cognitive flexibility
Authors: Yuan Yidan , He Xinyue , He Quanxing , Jia Yimeng , Xu Zhansheng , Li Man 
Published: December 2023

Abstract
Background
With the widespread adoption of smartphones, there has been a notable increase in problematic mobile phone use (PMPU), particularly prevalent among college students. Research suggests that apart from being associated with various problematic behaviors, this excessive mobile phone use might also have an impact on individual personality traits, such as time management disposition (TMD), which plays a significant role in individual motivation and psychological well-being. While previous literature has identified a negative relationship between PMPU and TMD, few studies have delved into the underlying mediating mechanism. Thus, the main aim of this study was to examine the chain mediating effect of sleep quality and cognitive flexibility on the relationship between PMPU and TMD.

Methods
A total of 921 Chinese college students completed the questionnaire. We collected basic information about the participants and assessed their PMPU, TMD, sleep quality, and cognitive flexibility using the Problematic Mobile Phone Use Scale-10, Adolescence Time Management Disposition Inventory, Pittsburgh Sleep Quality Index scale and Cognitive Flexibility Inventory.

Results
The results indicated a significant correlation among all the variables. Moreover, we noted that both sleep quality and cognitive flexibility fully mediated the association between PMPU and TMD. Additionally, a chain mediating effect involving sleep quality and flexibility in this relationship was also identified.

Conclusion
We found that sleep quality and cognitive flexibility had a series of multiple mediating effects in the pathway from PMPU to TMD, and both significantly mediated TMD. These findings indicated that impaired cognitive function and sleep quality may contribute to time management difficulties resulting from PMPU, suggesting that problematic behaviors like PMPU can impact one’s personality traits. Therefore, interventions should be enhanced to mitigate the adverse effects of PMPU.
S100A8/A9 as a risk factor for breast cancer negatively regulated by DACH1
Authors: Zhang Xiaojun , Niu Mengke , Li Tianye , Wu Yuze , Gao Jinnan , Yi Ming , Wu Kongming 
Published: December 2023

Abstract
Background
S100A8 and S100A9 are members of Ca2+-binding EF-hand superfamily, mainly expressed by macrophages and neutrophils. Limited by the poor stability of homodimers, they commonly exist as heterodimers. Beyond acting as antibacterial cytokines, S100A8/A9 is also associated with metabolic and autoimmune diseases such as obesity, diabetes, and rheumatoid arthritis. While the involvement of S100A8/A9 in breast cancer development has been documented, its prognostic significance and the precise regulatory mechanisms remain unclear.

Methods
S100A8/A9 protein in breast cancer samples was evaluated by immunohistochemistry staining with tumor tissue microarrays. The serum S100A8 concentration in patients was measured by enzyme-linked immunosorbent assay (ELISA). The S100A8 secreted by breast cancer cells was detected by ELISA as well. Pooled analyses were conducted to explore the relationships between S100A8/A9 mRNA level and clinicopathological features of breast cancer patients. Besides, the effects of S100A8/A9 and DACH1 on patient outcomes were analyzed by tissue assays. Finally, xenograft tumor assays were adopted to validate the effects of DACH1 on tumor growth and S100A8/A9 expression.

Results
The level of S100A8/A9 was higher in breast cancer, relative to normal tissue. Increased S100A8/A9 was related to poor differentiation grade, loss of hormone receptors, and Her2 positive. Moreover, elevated S100A8/A9 predicted a worse prognosis for breast cancer patients. Meanwhile, serum S100A8 concentration was upregulated in Grade 3, basal-like, and Her2-overexpressed subtypes. Additionally, the results of public databases showed S100A8/A9 mRNA level was negatively correlated to DACH1. Stable overexpressing DACH1 in breast cancer cells significantly decreased the generation of S100A8. The survival analysis demonstrated that patients with high S100A8/A9 and low DACH1 achieved the shortest overall survival. The xenograft models indicated that DACH1 expression significantly retarded tumor growth and downregulated S100A8/A9 protein abundance.

Conclusion
S100A8/A9 is remarkedly increased in basal-like and Her2-overexpressed subtypes, predicting poor prognosis of breast cancer patients. Tumor suppressor DACH1 inhibits S100A8/A9 expression. The combination of S100A8/A9 and DACH1 predicted the overall survival of breast cancer patients more preciously.