bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid LAMP assay for the diagnosis of oak wilt with the naked eye
Authors: Novi Vinni Thekkudan , Aboubakr Hamada A. , Moore Melanie J. , Juzwik Jennifer , Abbas Abdennour 
Published: February 2024

Abstract

Background
Oak wilt disease, caused by Bretziella fagacearum is a significant threat to oak (Quercus spp.) tree health in the United States and eastern Canada. Without management the disease may cause dramatic changes to natural and urban ecosystems. Early and accurate diagnosis followed by timely treatment increases the level of disease control success.
Results
A rapid assay based on loop mediated isothermal amplification (LAMP) was first developed with fluorescence detection of the target fungus after 30-minute reaction time. Six different primers were designed to specifically bind and amplify the pathogen’s DNA. To simplify the assay for “point of care” (i.e., field) use, gold nanoparticles (AuNPs) were designed to bind to the amplified DNA (amplicon) obtained from the LAMP reaction. Upon inducing precipitation, the AuNP-amplicons settle as a red pellet visible to the naked eye, indicative of pathogen presence. Both infected and healthy red oak samples were tested using this visualization method. The sensitivity and specificity of the assay were calculated to be 100%. Moreover, the developed assay was able to detect the pathogen in crude DNA extracts of diseased oak wood samples, which further reduced the time required to process samples.
Conclusions
In summary, the LAMP assay coupled with oligonucleotide-conjugated gold nanoparticle visualization is a promising method for accurate and rapid molecular-based diagnosis in field settings. The new method can be adapted to other forest and plant diseases by simply designing new primers.
Developmental odontogenic cysts with special focus on the occurrence of multiple cysts: a single-centre retrospective study from the Czech Republic
Authors: Szaraz David , Ksinan Albert J. , Machacek Ctirad , Linhartova Petra Borilova 
Published: February 2024

Abstract
Background
This retrospective study aims to evaluate the relative representation of individual types of developmental odontogenic cysts (DOCs), especially from the perspective of syndromic and non-syndromic multiple DOCs in the Czech population. In addition, we also summarize the previous studies on the occurrence of multiple DOCs as well as provide a literature review of case reports and case series on non-syndromic multiple DOCs, mainly dentigerous cysts and odontogenic keratocysts.
Methods
The study included histologicaly confirmed DOCs retrieved between January 1, 2012, and August 8, 2023, at the Clinic of Maxillofacial Surgery, University Hospital Brno, Czech Republic. All specimens were re-classified according to the fifth edition of the World Health Organization Classification of Head and Neck Tumors, 2022. Patients with an uncertain histological diagnosis were excluded from the study.
Results
Of a total of 377 patients, 286 had dentigerous cysts (DCs), 85 odontogenic keratocysts (OKCs), 5 orthokeratinizing odontogenic cysts (OOCs), 1 botryoid cyst and 1 calcifying odontogenic cyst. 19 multiple cysts were observed in the DC group, all without any association with a syndrome. Among the OKCs, 6 patients were diagnosed with the Naevoid Basal Cell Carcinoma Syndrome (NBCCS) and 3 were non-syndromic. Recurrence of OKCs was associated with NBCCS (p < 0.05). OKCs appeared more frequently in younger patients and predominantly in women, while the opposite was observed in DCs (p < 0.05 for all). Both these lesions affected the mandible in the vast majority of cases. Only one patient presented with bilateral OOCs, without any association with a syndrome.
Conclusion
Multiple OKCs are more likely to develop in syndromic patients, while none of the multiple DCs were associated with a syndrome. The incidence of multiple OOCs and other DOCs is extremely rare.
Delineation of Groundwater Potential and the Susceptibility to Surface Contaminants
Authors: Ango Abubakar , Emmanuel Jaiyeoba , Ekwu Mary T 
Published: February 2024

Abstract

Groundwater remains an essential natural resource that is found in every part of the Earth’s subsurface. However, the high level of borehole failures due to inadequate subsurface information about the regional hydrogeological nature has led to significant water scarcity in both rural and semi-urban regions. This study applies the vertical electrical sounding method to delineate regional aquifer potential alongside the vulnerability to the agrochemical activities in a rapidly emerging agricultural development at Kujama Farmland for groundwater development. Information obtained was used to construct the soil depth section and the groundwater potential as well as its protective capacity. The depth section shows that the study area is underlain by four distinctive geo-electric layers. The weathered/fractured unit was delineated as the water-bearing unit of the study area for groundwater development. The aquifer parameters such as resistivity and depth values vary between 21 Ωm – 328 Ωm and 19 m – 42 m, respectively. However, the observed thick aquifer with low resistivity values was considered as the region of the rich aquifer and it could supply sustainable water for drinking, domestic use and irrigation. Consequently, the siting of industrial/public/domestic boreholes should sunk to at least a depth of 30/20/10m respectively. On the other hand, the computed aquifer parameters show that the study is considered 75% highly protective, 22% moderately protective and 2% weakly protective from the possible surface contaminants emanating from the emerging agricultural activities at Kujama Multipurpose Farmland.
Сравнительный анализ эффективности режимов мобилизации аутологичных гемопоэтических стволовых клеток у больных множественной миеломой
Authors: Кострома Иван Иванович , Чубукина Ж. В. , Юдина В. А. , Романенко Н. А. , Степченкова Е. И. , Бессмельцев С. С. , Сидоркевич С. В. , Грицаев С. В. 
Published: February 2024

Aim. To compare the efficacy of mobilization regimens (MR) differing in their composition and intensity, with the purpose of defining the criteria to personalize the choice of MR based on clinical and hematological characteristics of multiple myeloma (MM) patients.
Materials & Methods. A retrospective analysis of the autologous hematopoietic stem cell (HSC) mobilization and autograft harvesting results was performed in 177 patients with newly diagnosed MM. The patients were divided into 4 groups. Group 1 included 62 patients with the median age of 53 years who were treated with single injection of cyclophosphamide (CF) dose 3 g/m2 as MR. Group 2 consisted of 71 patients with the median age of 58 years who received vinorelbine 35 mg/m2. Granulocyte colony-stimulating factor (G-CSF) as a monoregimen was administered to group 3 consisting of 33 patients with the median age of 55 years. Group 4 included 11 patients with the median age of 57 years who received G-CSF enhanced by plerixafor administration. G-CSF 10 µg/kg was used as MR. In all chemomobilization cases, daily G-CSF 10 µg/kg started on Day 4 from the administration of the chemotherapy drug prescribed as MR.
Results. In the analyzed groups, the median time from MR start to the first leukocyte apheresis session was 11, 8, 5, and 5 days, respectively. On the first leukocyte apheresis day, the median CD34+ cell collection in group 3 was significantly lower than in groups 1, 2, and 4: 2.2 × 106/kg vs. 3.79 × 106/kg, 7.22 × 106/kg, and 3.9 × 106/kg, respectively. The total CD34+ cell collection after two leukocyte apheresis sessions was also the lowest in group 3 compared with groups 1, 2, and 4: 3.22 × 106/kg vs. 5.2 × 106/kg, 4.95 × 106/kg, and 7.5 × 106/kg, respectively. In the analyzed groups, the rate of mobilization with CD34+ cell collection < 2.0 × 106/kg was 6.5 %, 5.6 %, 18.2 %, and 9.1 %. The evaluation of the results in all patients showed a direct correlation of CD34+ cell collection with lenalidomide administered before autologous HSC mobilization. A significant difference in CD34+ cell collection in lenalidomide recipients vs. non-recipients was reported when vinorelbine as MR and G-CSF as monoregimen (р = 0.001 and р = 0.022, respectively) were used. No significant differences were observed either with CF or G-CSF combined with plerixafor treatment.
Conclusion. Based on the findings, age of a MM patient, comorbidities, and prior lenalidomide administration can be regarded as key criteria for choosing one of 4 MRs.
Медикаментозная защита яичников в период проведения программной противоопухолевой лекарственной терапии у больных с классической лимфомой Ходжкина и неходжкинскими лимфомами
Authors: Антух И. Е. , Шпирко Валерия Олеговна , Назаренко Т. А. , Мартиросян Я. О. , Бирюкова А. М. , Хохлова С. В. , Тумян Г. С. 
Published: February 2024

Aim. To assess the efficacy of ovarian protection and options for fertility preservation with the use of gonadotropin-releasing hormone agonists (a-GnRH) in patients with classical Hodgkin lymphoma (cHL) and non-Hodgkin lymphomas (NHLs) during program drug chemotherapy.
Materials & Methods. The study enrolled 247 female patients (187 with cHL and 60 with NHLs) undergoing program drug chemotherapy from 2019 to 2023. The patients were aged 13–42 years (median 24 years). Prior to chemotherapy and after it was completed, the serum anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH), and estradiol levels were measured. Besides, antral follicle count was estimated by pelvic ultrasound. To provide ovarian protection during chemotherapy, a-GnRH was administered to 67 (27 %) out of 247 patients. Ovarian reserve was assessed based on ultrasound and AMH, FSH, and estradiol levels in 2–3 months after completing the program of drug chemotherapy or after spontaneous menstrual recovery.
Results. Menstrual cycle recovered in 194 (78.5 %) out of 247 patients, regardless of lymphoid malignancy variant. Among them, there were 79.7 % (n = 149) of cHL patients and 75 % (n = 45) of NHL patients. Accordingly, ovarian function was lost in 20.3 % (n = 38) of cHL patients and 25 % (n = 15) of NHL patients. Predictors of ovarian function recovery appeared to be age ≤ 28 years and AMH level > 2.45 ng/mL. Pharmacological protection of ovaries did not impact the probability of menstrual cycle recovery. Regardless of immunomorphological variant of lymphoid malignancy, a regular menstrual cycle was completely restored in 48 (71.6 %) out of 67 a-GnRH recipients and in 146 (81.1 %) out of 180 patients without a-GnRH treatment.
Conclusion. Most of cHL and NHL patients of early reproductive age (≤ 30 years) have a fair chance of menstrual cycle recovery after completing the program drug chemotherapy similar to BEACOPP, EACODD(PP)-14, R-CHOP, R-Da-EPOCH and other regimens. Ovarian reserve cannot be preserved by means of a-GnRH administration during chemotherapy. High baseline FSH and low baseline AMH levels indirectly indicate evidence of impaired ovarian reserve. Patients belonging to this category are those who, if intensive combined cytostatic therapy regimens are planned, should undergo prior oocyte/embryo retrieval with subsequent cryopreservation due to the risk of premature loss of ovarian function.
Forecasting of water quality parameters of Sandia station in Narmada basin, Central India, using AI techniques
Authors: Tiwari Deepak Kumar , Singh K. R. , Kumar Vijendra 
Published: February 2024

Abstract

In addition to the influence of climate change on water availability and hydrological risks, the effects on water quality are in the early stages of investigation. This study aims to consolidate the latest interdisciplinary research in the application of artificial intelligence (AI) in the field of assessment of water quality parameters and its prediction. This research paper specifically explores the intricate relationship between climate change and water quality parameters at Sandia station, situated within the Narmada basin in Central India. As global climatic patterns continue to shift, the repercussions on water resources have gained prominence. In this work, electrical conductivity is predicted using the KERAS data processing environment on TensorFlow. The root-mean-square error (RMSE), coefficient of determination (R2), Nash–Sutcliffe efficiency (NSE), etc. are calculated between observed and predicted values to assess the model performance. A total of ten models are developed depending upon the input geometry from past monthly timelines. The results indicate that model 8, with ten inputs, performs the best based on the R2 value of 0.889. These results indicate that AI can be very helpful in analyzing the possible threats in the future for drinking water, livestock feeding, irrigation, and so on.
Greenhouse gas emissions from sanitation and wastewater management systems: a review
Authors: Lambiasi Layla , Ddiba Daniel , Andersson Kim , Parvage Masud , Dickin Sarah 
Published: February 2024

ABSTRACT

There is growing awareness of the contribution of sanitation systems to greenhouse gas (GHG) emissions globally, and hence to climate change. However, there is a lack of comprehensive insight into emission sources disaggregated across the entire sanitation chain. This study presents a detailed review and analysis of emission sources from both sewer-based and non-sewered sanitation systems, with a focus on both fugitive emissions and those related to system operation. Our analysis highlights evidence gaps in several areas in the literature: quantifying emissions from non-sewered sanitation systems, with particular gaps related to technologies like biogas toilets and composting toilets; oversight of contextual factors such as environmental conditions and infrastructure operational status in GHG accounting; a dearth of holistic GHG emission studies across the entire sanitation chain comparable to those in the solid waste management sector; and inconsistencies in GHG measurement methods. By pinpointing these gaps, this review provides a robust reference for planning climate mitigation strategies for sanitation and wastewater management systems, emphasizes the urgent need for the incorporation of climate-smart solutions in the sector, e.g. in the design of new and retrofitted infrastructure, and aims to bridge the sustainable development goals related to sanitation and climate action.
Analisis Pendapatan dan Kelayakan Usahatani Jagung Nasa 29 di Kecamatan Pamona Puselemba Kabupaten Poso
Authors: Pamusu Sherley Siseraf , Paelo Yunus 
Published: February 2024

<p>Jagung merupakan komoditas tanaman pangan yang dapat dipergunakan sebagai pengganti padi, selain itu juga dapat dipergunakan sebagai pakan ternak. Pengembangan jagung nasa 29 merupakan suatu program akselerasi pengembangan komoditas unggulan daerah Sulawesi Tengah khususnya Kabupaten Poso yang dirancang sebagai suatu upaya terobosan yang diyakini mampu memberikan kontribusi yang tinggi pada peningkatan pendapatan masyarakat khususnya pelaku agribisnis jagung dari hulu sampai hilir, hal ini sesuai dengan program Kementerian Pertanian. Penelitian ini bertujuan sebagai berikut: 1. Untuk mengetahui besarnya pendapatan pada usahatani jagung satu kali musim tanam di Kecamatan Pamona Puselemba Kabupaten Poso, 2. Untuk mengetahui besarnya R/C pada usahatani jagung satu kali musim tanam di Kecamatan Pamona Puselemba Kabupaten Poso. Sampel petani yang digunakan sebanyak 55 responden dan pengumpulan data menggunakan teknik<em> random sampling method. </em> Hasil penelitian menunjukkan bahwa rata-rata pendapatan petani jagung dalam satu musim tanam sebesar Rp 16.554.666, 00 dan R/C ratio usahatani sebesar 3,33 hal ini menunjukkan bahwa usahatani jagung nasa 29 di Kecamatan Pamona Puselemba Kabupaten Poso layak diusahakan untuk mencapai kesejahteraan petani.</p><p> </p><p> </p><p><strong>Kata Kunci:</strong> jagung nasa 29, pendapatan, analisis lelayakan, usahatani</p><p> </p>
Resistensi Perempuan terhadap Kegiatan Pertambangan Emas di Gunung Tumpang Pitu Kabupaten Banyuwangi
Authors: Mariati Tia , Yuliati Yayuk , Sukesi Keppi 
Published: February 2024

<p>Desa Sumberagung merupakan kawasan yang memiliki potensi wisata laut dan sumberdaya alam berupa emas yang terletak di Gunung Tumpang Pitu. Pertambangan mulai tahun 2015 sampai tahun 2022 berada dibawah anak perusahaan PT Indo Multi Niaga yaitu PT BSI. Permasalahan penelitian : resistensi masyarakat Desa Sumberagung terhadap IUP PT BSI dan PT. DSI dalam kegiatan eksploitasi Gunung Tumpang Pitu. Tujuan Penelitian melihat resistensi yang dilakukan masyarakat Desa Sumberagung terkait usaha mencabut IUP PT. BSI dan PT. DSI. Rumusan masalah penelitian 1) Bagaimana dampak kegiatan penambangan emas bagi masyarakat Desa Sumberagung? (2) Bagaimana proses munculnya konflik antara masyarakat dengan pihak perusahaan (3) Bagaimana resistensi perempuan terhadap kegiatan eksplotasi tambang emas di Gunung Tumpang Pitu? Penelitian ini mengunakan pendekatan kualitatif deskriptif dengan menggunakan metode studi kasus. Penentuan informan menggunakan <em>Snowball sampling. </em>Analisis data menggunakan komponen analisis Milles and Huberman 2014 model interaktif. Hasil penelitian 1) )Kerusakan diberbagai sector: lingkungan yaitu hilangnya hutan lindung Gunung Tumpang Pitu,, hilangnya endemic hewan dan tumbuhan dan pencemaran air yang mengandung sianida, banjir lumpur, hilangnya sungai katakana, degdradasi tanah. Sector ekonomi : gagal panen akibat banjir lumpur yang masuk kedalam pertanian, hasil tangkapan nelayan yang mulai berkurang sehingga nelayan harus jauh melaut sampai Bali dan Jember untuk mendapatkan hasil yang maksimal. Sektor sosial kebising, konflik masyarakat dengan pemerintah dan perusahaan 2) Munculnya konflik dimulai dari diizinkanya PT Hakman Metalindo untuk melakukan tinjauan kandungan emas mulai dari Jember sampai Banyuwangi selama 1 tahun 3) resitensi berupa : Demonstrasi, kayuh sepeda sampai Surabaya, aksi istigosah, aksi pengahadangan alat berat, pembangunan posko penghadangan di pintu masuk Desa Pancer</p>
Seberapa Elastis Permintaan Bawang Putih Indonesia? Sebuah Analisis Regresi Linier Berganda
Authors: Noviaranti Shavira Putri , Zainuddin Ahmad 
Published: February 2024

<p>Bawang putih (<em>Allium sativum</em>) merupakan salah satu komoditas pertanian yang sangat dibutuhkan di Indonesia. Bawang putih merupakan salah satu komoditas yang memiliki banyak manfaat. Bawang putih bukan hanya dimanfaatkan sebagai bumbu masak namun juga sebagai obat-obatan. Permintaan bawang putih di Indonesia selama ini terus mengalami peningkatan. Tujuan penelitian ini yaitu untuk mengetahui faktor-faktor apa saja yang mempengaruhi permintaan bawang putih di Indonesia dan bagaimana proyeksi permintaan bawang putih di Indonesia pada 10 tahun yang akan datang (tahun 2022-2031). Variabel yang digunakan pada penelitian yaitu permintaan bawang putih, jumlah penduduk, pendapatan nasional perkapita, harga riil bawang putih dan harga riil bawang merah di tingkat konsumen. Data yang digunakan dalam penelitian merupakan data <em>time series</em> dari tahun 1985-2020. Metode analisis yang digunakan yaitu analisis regresi berganda dan metode analisis ARIMA (<em>Autoregressive Integrated Moving Average</em>). Hasil penelitian menunjukkan faktor-faktor yang mempengaruhi permintaan bawang putih di Indonesia yaitu pendapatan nasional perkapita dengan koefisien elastisitas 9,14 dan harga riil bawang putih di tingkat konsumen dengan koefisien elastisitas -2,65. Elastisitas permintaan bawang putih di Indonesia dapat dikatakan Elastis. Hasil proyeksi permintaan bawang putih di Indonesia pada 10 tahun yang akan datang (tahun 2022-2031) mengalami perkembangan yang meningkat atau trend positif. Rata-rata pertumbuhan proyeksi permintaan yaitu 1,81% per tahun.</p>
Analisis Risiko Usahatani Bawang Merah Di Desa Banti Kecamatan Baraka Kabupaten Enrekang
Authors: Putra Chairil Arun , Nurdin Nurdin , Akbar Akbar 
Published: February 2024

<p>Usahatani bawang merah merupakan salah satu komoditas pertanian yang memiliki nilai ekonomi tinggi dan strategis. Tetapi sebagaimana komoditi pertanian lainnya bawang merah juga tidak luput dari yang namanya risiko, tingkat risiko yang terjadi akan mempengaruhi besarnya pendapatan yang akan diterima petani. Penelitian ini bertujuan mengukur tingkat risiko produksi, risiko biaya dan risiko pendapatan usahatani bawang merah di Desa Banti Kecamatan Baraka Kabupaten Enrekang. Metode penelitian yang digunakan adalah <em>simple random sampling, </em>jumlah sampel yang diambil sebanyak 10% dari 300 petani bawang merah di Desa Banti Kecamatan Baraka Kabupaten Enrekang yaitu sebanyak 30 sampel petani bawang merah dan data yang digunakan adalah data primer dan data sekunder. Analisis data yang digunakan adalah analisis tingkat risiko menggunakan Koefisien Variasi (CV). Hasil penelitian menunjukkan bahwa risiko produksi sebesar 0,11 (11%), risiko biaya sebesar 0,08 (8%) dan risiko pendapatan sebesar 0,74 (74%). Tingkat risiko produksi, risiko biaya dan risiko pendapatan di Desa Banti Kecamatan Baraka Kabupaten Enrekang tergolong rendah dikarenakan nilai Koefesien Variasinya (CV)&lt;1.</p>
Patient and Therapist Perceptions of a Publicly Funded Internet-Based Cognitive Behavioral Therapy (iCBT) Program for Ontario Adults During the COVID-19 Pandemic: Qualitative Study
Authors: Thapar Serena , Nguyen Megan , Khan Bilal Noreen , Fanaieyan Roz , Kishimoto Vanessa , Liu Rebecca , Bolea-Alamañac Blanca , Leon-Carlyle Marisa , O’Riordan Anne , Keresteci Maggie , Bhattacharyya Onil 
Published: February 2024

Background
To address the anticipated rise in mental health symptoms experienced at the population level during the COVID-19 pandemic, the Ontario government provided 2 therapist-assisted internet-delivered cognitive behavioral therapy (iCBT) programs to adults free of charge at the point of service.

Objective
The study aims to explore the facilitators of and barriers to implementing iCBT at the population level in Ontario, Canada, from the perspective of patients and therapists to better understand how therapist-assisted iCBT programs can be effectively implemented at the population level and inform strategies for enhancing service delivery and integration into the health care system.

Methods
Using a convenience sampling methodology, semistructured interviews were conducted with 10 therapists who delivered iCBT and 20 patients who received iCBT through either of the publicly funded programs to explore their perspectives of the program. Interview data were analyzed using inductive thematic analysis to generate themes.

Results
Six salient themes were identified. Facilitators included the therapist-assisted nature of the program; the ease of registration and the lack of cost; and the feasibility of completing the psychoeducational modules given the online and self-paced nature of the program. Barriers included challenges with the online remote modality for developing the therapeutic alliance; the program’s generalized nature, which limited customization to individual needs; and a lack of formal integration between the iCBT program and the health care system.

Conclusions
Although the program was generally well-received by patients and therapists due to its accessibility and feasibility, the digital format of the program presented both benefits and unique challenges. Strategies for improving the quality of service delivery include opportunities for synchronous communication between therapists and patients, options for increased customization, and the formal integration of iCBT into a broader stepped-care model that centralizes patient referrals between care providers and promotes continuity of care.