bluuebunny/crossref_metadata_2025_split huggingface dataset:
A landscape-scale field survey demonstrates the role of wheat volunteers as a local and diversified source of leaf rust inoculum
Authors: Boixel A.-L. , Goyeau H. , Berder J. , Moinard J. , Suffert F. , Soubeyrand S. , Sache I. , Vidal T. 
Published: November 2023

AbstractDeploying disease-resistant cultivars is one of the most effective control strategies to manage crop diseases such as wheat leaf rust, caused by Puccinia triticina. After harvest, this biotrophic fungal pathogen can survive on wheat volunteers present at landscape scale and constitute a local source of primary inoculum for the next cropping season. In this study, we characterised the diversity of P. triticina populations surveyed on wheat volunteer seedlings for six consecutive years (2007–2012) at the landscape scale. A total of 642 leaf rust samples classified in 52 virulence profiles (pathotypes) were collected within a fixed 5-km radius. The pathotype composition (identity and abundance) of field-collected populations was analyzed according to the distance between the surveyed wheat plots and to the cultivars of origin of isolates. Our study emphasised the high diversity of P. triticina populations on wheat volunteers at the landscape scale. We observed an impact of cultivar of origin on pathogen population composition. Levels of population diversity differed between cultivars and their deployment in the study area. Our results suggest that wheat volunteers could provide a significant though highly variable contribution to the composition of primary inoculum and subsequent initiation of leaf rust epidemics.
Glass/steel/clay interactions in a simulated radioactive waste geological disposal system
Authors: Tolnai I. , Osan J. , Czompoly O. , Sulyok A. , Fabian M. 
Published: November 2023

AbstractDeep geological storage is the accepted solution for the final disposal of high-level radioactive waste therefore, it is necessary to study the host rock of the planned Hungarian waste repository and the materials involved in the engineered barriers. The main goal was to understand the characteristics and stability of the glass/steel/claystone system, from the structural properties of the vitrified waste (borosilicate glasses) to the clay response in the repository. Repository conditions were applied during the experiments to understand the chemical evolution of the system. A triplicate setup was kept at 80 °C for 3, 7 and 12 months and post-mortem characterization was performed. No alteration products were observed with scanning electron microscopy energy dispersive X-ray spectroscopy measurements on the surface of the glass and Fe or in the clay after the end of the experimental period. Based on the elemental analysis of the liquid phase, the released amount of B, K, Si and Na increased, while that of Ca and Mg decreased compared to the baseline. The concentrations of Cl− and SO42− did not change significantly. Ca- and Mg-silicate precipitation was observed by X-ray photoelectron spectroscopy at the surface range of the borosilicate glasses because of the synthetic porewater treatment.
A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care
Authors: Chang Megan M. , Ma Ariel , Novak Emilie Newsham , Barra Maria , Kundrod Kathryn A. , Montealegre Jane Richards , Scheurer Michael E. , Castle Philip E. , Schmeler Kathleen , Richards-Kortum Rebecca 
Published: November 2023

AbstractCervical cancer is a leading cause of death for women in low-resource settings despite being preventable through human papillomavirus (HPV) vaccination, early detection, and treatment of precancerous lesions. The World Health Organization recommends high-risk HPV (hrHPV) as the preferred cervical cancer screening strategy, which is difficult to implement in low-resource settings due to high costs, reliance on centralized laboratory infrastructure, and long sample-to-answer times. To help meet the need for rapid, low-cost, and decentralized cervical cancer screening, we developed tailed primer isothermal amplification and lateral flow detection assays for HPV16, HPV18, and HPV45 DNA. We translated these assays into a self-contained cartridge to achieve multiplexed detection of three hrHPV genotypes in a disposable cartridge. The developed test achieves clinically relevant limits of detection of 50–500 copies per reaction with extracted genomic DNA from HPV-positive cells. Finally, we performed sample-to-answer testing with direct lysates of HPV-negative and HPV-positive cell lines and demonstrated consistent detection of HPV16, HPV18, and HPV45 with 5000–50,000 cells/mL in < 35 min. With additional optimization to improve cartridge reliability, incorporation of additional hrHPV types, and validation with clinical samples, the assay could serve as a point-of-care HPV DNA test that improves access to cervical cancer screening in low-resource settings.
Paleoenvironments of Late Devonian tetrapods in China
Authors: Guo Xuelian , Retallack Gregory J. , Liu Jinhao 
Published: November 2023

AbstractThe major evolutionary transition from fish to amphibian included Late Devonian tetrapods that were neither fish nor amphibian. They had thick necks and small limbs with many digits on elongate flexuous bodies more suitable for water than land. Habitats of Devonian tetrapods are of interest in assessing selective pressures on their later evolution for land within three proposed habitats: 1, tidal flats, 2, desert ponds, and 3, woodland streams. Here we assess paleoenvironments of the Late Devonian tetrapod Sinostega from paleosols in Shixiagou Canyon near Zhongning, Ningxia, China. Fossil tetrapods, fish, molluscs, and plants of the Zhongning Formation are associated with different kinds of paleosols, representing early successional vegetation, seasonal wetlands, desert shrublands, and riparian woodlands, and paleoclimates ranging from semiarid moderately seasonal to monsoonal subhumid. The tetrapod Sinostega was found in a paleochannel of a meandering stream below a deep-calcic paleosol supporting well drained progymnosperm woodland in a monsoonal subhumid paleoclimate. This habitat is similar to that of the tetrapods Densignathus, Hynerpeton, and an indeterminate watcheeriid from Pennsylvania, USA. Chinese and Pennsylvanian Late Devonian tetrapods lived in productive woodland streams, choked with woody debris as a refuge from large predators. Habitats of other Devonian tetrapods have yet to be assessed from studies of associated paleosols as evidence for their ancient climate and vegetation.
Preclinical evidence for the effective use of TL-895, a highly selective and potent second-generation BTK inhibitor, for the treatment of B-cell malignancies
Authors: Goodstal Samantha M. , Lin Jing , Crandall Timothy , Crowley Lindsey , Bender Andrew T. , Pereira Albertina , Soloviev Maria , Wesolowski John S. , Iadevaia Riham , Schelhorn Sven-Eric , Ross Edith , Morandi Federica , Ma Jianguo , Clark Anderson 
Published: November 2023

AbstractTL-895 (formerly known as M7583) is a potent, highly selective, adenosine triphosphate (ATP)-competitive, second-generation, irreversible inhibitor of Bruton’s tyrosine kinase (BTK). We characterized its biochemical and cellular effects in in vitro and in vivo models. TL-895 was evaluated preclinically for potency against BTK using IC50 concentration–response curves; selectivity using a 270-kinase panel; BTK phosphorylation in Ramos Burkitt’s lymphoma cells by ProteinSimple Wes analysis of one study; anti-proliferative effects in primary chronic lymphocytic leukemia (CLL) blasts; cell viability effects in diffuse large B-cell lymphoma (DLBCL) and mantle-cell lymphoma (MCL) cell lines; effects on antibody-dependent cell-mediated cytotoxicity (ADCC) from Daudi cells and chromium-51 release from human tumor cell lines; and efficacy in vivo using four MCL xenograft model and 21 DLBCL patient-derived xenograft (PDX) models (subtypes: 9 ABC, 11 GCB, 1 Unclassified). TL-895 was active against recombinant BTK (average IC50 1.5 nM) and inhibited only three additional kinases with IC50 within tenfold of BTK activity. TL-895 inhibited BTK auto-phosphorylation at the Y223 phosphorylation site (IC50 1–10 nM). TL-895 inhibited the proliferation of primary CLL blasts in vitro and inhibited growth in a subset of activated DLBCL and MCL cell lines. TL-895 inhibited the ADCC mechanism of therapeutic antibodies only at supra-clinical exposure levels. TL-895 significantly inhibited tumor growth in the Mino MCL xenograft model and in 5/21 DLBCL PDX models relative to vehicle controls. These findings demonstrate the potency of TL-895 for BTK and its efficacy in models of B-cell lymphoma despite its refined selectivity.
Pupil dilation response elicited by violations of auditory regularities is a promising but challenging approach to probe consciousness at the bedside
Authors: Sangare Aude , Quirins Marion , Marois Clémence , Valente Mélanie , Weiss Nicolas , Perez Pauline , Ben Salah Amina , Munoz-Musat Esteban , Demeret Sophie , Rohaut Benjamin , Sitt Jacobo D. , Eymond Cecile , Naccache Lionel 
Published: November 2023

AbstractPupil dilation response (PDR) has been proposed as a physiological marker of conscious access to a stimulus or its attributes, such as novelty. In a previous study on healthy volunteers, we adapted the auditory “local global” paradigm and showed that violations of global regularity elicited a PDR. Notably without instructions, this global effect was present only in participants who could consciously report violations of global regularities. In the present study, we used a similar approach in 24 non-communicating patients affected with a Disorder of Consciousness (DoC) and compared PDR to ERPs regarding diagnostic and prognostic performance. At the group level, global effect could not be detected in DoC patients. At the individual level, the only patient with a PDR global effect was in a MCS and recovered consciousness at 6 months. Contrasting the most regular trials to the most irregular ones improved PDR’s diagnostic and prognostic power in DoC patients. Pupillometry is a promising tool but requires several methodological improvements to enhance the signal-to-noise ratio and make it more robust for probing consciousness and cognition in DoC patients.
Behaviourally-informed household communications increase uptake of radon tests in a randomised controlled trial
Authors: Timmons Shane , Lunn Peter D. 
Published: November 2023

AbstractExposure to radon gas is a leading cause of lung cancer. Testing homes for the gas is straightforward, yet most people do not undertake tests even when offered freely. We report a pre-registered randomised controlled trial of communications to encourage test uptake. Households (N = 3500) in areas at high risk of radon exposure were randomly assigned to receive (i) a the control letter from the national Environmental Protection Agency; (ii) a behaviourally-informed version of the control letter that incorporated multiple nudges, including reciprocity messages and numeric frequencies of risk; (iii) this same behaviourally-informed letter in a re-designed envelope; (iv) the behaviourally-informed letter in the re-designed enveloped with a radon risk map of the household’s county. The behaviourally-informed letter led to a large increase in test uptake, from 22% in the control condition to 33% (a 50% increase). There was no additional benefit of the re-designed envelope, which generated uptake of 30%. Including the map led some households to respond faster, but the overall uptake (26%) was weaker. The results have implications for public health communications with households and show the potential for techniques from behavioural science to help mitigate environmental risks.
Design and analysis of a new type of mobile ice cooling equipment for deep mine
Authors: Zhao Xingdong , Zhao Siyu , Li Ang 
Published: November 2023

AbstractIn the background environment of the serious problem of high temperature heat damage in deep mining, some mines have complex and interlocking forms of roadway arrangement, with the innovative concept of cooling on demand as the principle, this paper develops a mobile ice cooling equipment, and introduces and explains the equipment from the perspective of principle, composition and dimensions. and uses Comsol simulation software to simulate and analyze the main heat exchange process of the mobile ice cooling equipment under the conditions of two cooling sources, obtains quantitative results on the finned tube arrangement parameters and the heat exchange cooling effect of the equipment under ideal conditions, which provides data for the optimization and upgrading of this mobile ice cooling equipment. The results show that the mobile cooling equipment is capable of feeding the desired temperature of the cooling air into deep mine, and with flexible, convenient, efficient, and cost effective. This research and development is a new exploration of deep ventilation and cooling technology and equipment means, puts forward a new concept, accumulates valuable experience, and lays the foundation for the subsequent related research and optimization.
Natural language processing system for rapid detection and intervention of mental health crisis chat messages
Authors: Swaminathan Akshay , López Iván , Mar Rafael Antonio Garcia , Heist Tyler , McClintock Tom , Caoili Kaitlin , Grace Madeline , Rubashkin Matthew , Boggs Michael N. , Chen Jonathan H. , Gevaert Olivier , Mou David , Nock Matthew K. 
Published: November 2023

AbstractPatients experiencing mental health crises often seek help through messaging-based platforms, but may face long wait times due to limited message triage capacity. Here we build and deploy a machine-learning-enabled system to improve response times to crisis messages in a large, national telehealth provider network. We train a two-stage natural language processing (NLP) system with key word filtering followed by logistic regression on 721 electronic medical record chat messages, of which 32% are potential crises (suicidal/homicidal ideation, domestic violence, or non-suicidal self-injury). Model performance is evaluated on a retrospective test set (4/1/21–4/1/22, N = 481) and a prospective test set (10/1/22–10/31/22, N = 102,471). In the retrospective test set, the model has an AUC of 0.82 (95% CI: 0.78–0.86), sensitivity of 0.99 (95% CI: 0.96–1.00), and PPV of 0.35 (95% CI: 0.309–0.4). In the prospective test set, the model has an AUC of 0.98 (95% CI: 0.966–0.984), sensitivity of 0.98 (95% CI: 0.96–0.99), and PPV of 0.66 (95% CI: 0.626–0.692). The daily median time from message receipt to crisis specialist triage ranges from 8 to 13 min, compared to 9 h before the deployment of the system. We demonstrate that a NLP-based machine learning model can reliably identify potential crisis chat messages in a telehealth setting. Our system integrates into existing clinical workflows, suggesting that with appropriate training, humans can successfully leverage ML systems to facilitate triage of crisis messages.
Diselenide-bond replacement of the external disulfide bond of insulin increases its oligomerization leading to sustained activity
Authors: Arai Kenta , Okumura Masaki , Lee Young-Ho , Katayama Hidekazu , Mizutani Kenji , Lin Yuxi , Park Sam-Yong , Sawada Kaichiro , Toyoda Masao , Hojo Hironobu , Inaba Kenji , Iwaoka Michio 
Published: November 2023

AbstractSeleno-insulin, a class of artificial insulin analogs, in which one of the three disulfide-bonds (S-S’s) of wild-type insulin (Ins) is replaced by a diselenide-bond (Se-Se), is attracting attention for its unique chemical and physiological properties that differ from those of Ins. Previously, we pioneered the development of a [C7UA,C7UB] analog of bovine pancreatic insulin (SeIns) as the first example, and demonstrated its high resistance against insulin-degrading enzyme (IDE). In this study, the conditions for the synthesis of SeIns via native chain assembly (NCA) were optimized to attain a maximum yield of 72%, which is comparable to the in vitro folding efficiency for single-chain proinsulin. When the resistance of BPIns to IDE was evaluated in the presence of SeIns, the degradation rate of BPIns became significantly slower than that of BPIns alone. Furthermore, the investigation on the intermolecular association properties of SeIns and BPIns using analytical ultracentrifugation suggested that SeIns readily forms oligomers not only with its own but also with BPIns. The hypoglycemic effect of SeIns on diabetic rats was observed at a dose of 150 μg/300 g rat. The strategy of replacing the solvent-exposed S-S with Se-Se provides new guidance for the design of long-acting insulin formulations.
A 50-year cycle of sea surface temperature regulates decadal precipitation in the tropical and South Atlantic region
Authors: Gorenstein Iuri , Wainer Ilana , Pausata Francesco S. R. , Prado Luciana F. , Khodri Myriam , Dias Pedro L. Silva 
Published: November 2023

AbstractNortheast Brazil and Western Africa are two regions geographically separated by the Atlantic Ocean, both home to vulnerable populations living in semi-arid areas. Atlantic Ocean modes of variability and their interactions with the atmosphere are the main drivers of decadal precipitation in these Atlantic Ocean coastal areas. How these low-frequency modes of variability evolve and interact with each other is key to understanding and predicting decadal precipitation. Here we use the Self-Organizing Maps neural network with different variables to unravel causality between the Atlantic modes of variability and their interactions with the atmosphere. Our study finds an 82% (p<0.05) anti-correlation between decadal rainfall in Northeast Brazil and Western Africa from 1979 to 2005. We also find three multi-decadal cycles: 1870-1920, 1920-1970, and 1970-2019 (satellite era), pointing to a 50-year periodicity governing the sea surface temperature anomalies of Tropical and South Atlantic. Our results demonstrate how Northeast Brazil and Western Africa rainfall anti-correlation was formed in the satellite era and how it might be part of a 50-year cycle from the Tropical and South Atlantic decadal variability.
Bradypus tridactylus – „Eigentlich kann ich nicht
schneller“
Authors: Kellinghaus Heinz 
Published: November 2023

ZusammenfassungDer Fall einer 70-jährigen Patientin mit langjähriger, lokal
nicht erfolgreich zu behandelnder Psoriasis wird geschildert. Das Heilmittel
Bradypus tridactylus war bisher nicht geprüft worden. Es wurde gefunden
durch Verstehen der Sprache des Körpers, der Psychosomatik und mithilfe
der Intuition.
The use of dietary supplements and vitamin consumption during and after the Covid pandemic in Vietnam: a perspective of user-generated content
Authors: Ha Minh-Tri , Nguyen Giang-Do , Nguyen Thi Huong-Thanh , Thi Nguyen Bich-Duyen 
Published: November 2023

AbstractThis paper aims to examine the key determinants of User-Generated Content (UGC) affecting consumers’ purchase intention toward buying vitamin and dietary supplement products during the coronavirus disease (COVID-19) outbreak through the social networking site, Facebook. Five determinants of UGC have been investigated in this context to highlight the association with the purchase intention of Vietnamese consumers these days, including Quality, Quantity, Trust, Utilitarian Value, and Hedonic Value of generated content by experienced users. This cross-sectional study collected data through an online survey questionnaire among 331 respondents from three different regions throughout the whole of Vietnam. The results show that Quality, Quantity, Trust, and Utilitarian Value of UGC have positive relationships with consumers’ intention to buy vitamin and dietary supplement products on Facebook during the COVID-19 pandemic, while the Hedonic Value of UGC is not associated with their behavioral intention of consumption. Lastly, the findings are expected to contribute valuable knowledge about COVID-19 from UGC and its consequences on health and selected supplement products for recent emerging changes. Finally, the results are also a solid premise for future research to enlarge the scale of situational issues and perspectives during the global COVID-19 pandemic.
Propuesta metodológica para determinar el núcleo semántico de los gestos emblemáticos
Authors: Belío-Apaolaza Helena S. 
Published: November 2023

Resumen
En la configuración de los gestos emblemáticos o emblemas intervienen cuatro elementos: la forma, el significado, el uso y las unidades léxicas asociadas. Estos elementos se relacionan intrínseca y dependientemente en la comunicación, pero no de manera unívoca: para un mismo gesto pueden aparecer diferentes exponentes verbales que comparten el mismo significado. El objetivo de este trabajo es determinar una metodología que establezca el núcleo semántico de estos gestos, de manera que pueda utilizarse como denominación homogénea y sistemática a la hora de referirnos a ellos en investigaciones, repertorios y diccionarios kinésicos. Para ello, se proponen una nómina de criterios no excluyentes y se lleva a cabo la propuesta para encontrar el núcleo semántico de 25 emblemas del español de España. Específicamente, se considera la relación icónica entre el significado y la forma de cada gesto, se elabora una prueba de reconocimiento y denominación con hablantes nativos, se examinan diez inventarios gestuales y se analiza la presencia y marca lingüística de las diferentes unidades léxicas asociadas a cada emblema en cinco diccionarios de la lengua española. A partir de los anteriores, se propone una denominación para cada uno de los 25 gestos analizados.
Experimental study of the influences of surface roughness and thermal interface material on the performance of a thermoelectric generator
Authors: Fu Qiang , Wang Haitao , Li Ding , Liang Weiyu 
Published: November 2023

Abstract
Reducing surface roughness and using thermal interface materials (TIMs) at the interfaces between a thermoelectric generator (TEG), heat source, and heat sink are effective strategies for decreasing the thermal contact resistance (TCR) and enhancing the TEG performance. To evaluate the influences of parameters such as the surface roughness, the thermal conductivity of TIM and loading pressure, we conducted experiments to measure the open-circuit voltage and output power of the TEG under various installation conditions. We also analysed the changes in TCR and temperature difference across the TEG module. The experimental findings were validated with numerical simulations using COMSOL Multiphysics under specific conditions. Our results revealed that reducing surface roughness and using TIM could substantially reduce the TCR, increase the temperature difference across the TEG, and increase the output power from the TEG. In our experiments, we used a temperature controller, cartridge heaters and thermocouples to regulate and record the temperatures of the heat source and heat sink. When maintaining a temperature difference of 53 K between the heat source and heat sink, and loading pressure set at 0.2 MPa, without using TIM, as the surface roughness decreased from 2.2 μm to 0.37 μm and to 0.03 μm, leading to a reduction in the TCR from 0.22 K W−1 to 0.17 K W−1 and to 0.13 K/W. Simultaneously, the open-circuit voltage increased from 1.32 V to 1.65 V and to 1.86 V, and the maximum output power increased from 0.26 W to 0.44 W and to 0.58 W. Additionally, when the surface roughness was 0.37 μm, after using TIM with thermal conductivity of 1 W/m-K, 2 W m−1-K−1, and 5 W m−1-K−1, the open-circuit voltage reached 1.44 V, 1.74 V and 1.94 V, respectively, and the maximum power reached 0.31 W, 0.51 W and 0.65 W, respectively.