bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of Body Colour, Symbionts and Genomic Regions on the Pea Aphid Wing Plasticity Variation
Authors: Gregory Lauren E. , Driscoll Rose M. H. , Parker Benjamin J. , Brisson Jennifer A. 
Published: February 2025

ABSTRACTAdaptive phenotypic plasticity describes the phenomenon in which a single genotype can produce a variety of phenotypes that match their environments. Like any trait, plasticity is a phenotype that can exhibit variation, but despite the ecological importance of plasticity variation, little is known about its genetic basis. Here we use the pea aphid to investigate the genetic basis of wing plasticity variation. Previous reports have suggested an ecological association between body coloration and wing plasticity strength in the pea aphid, so we tested the hypothesis that the body colour determination locus (tor) associated with wing plasticity variation. We discover that there is no relationship between body colour and wing plasticity in natural populations or in a genetic mapping population. We also localise the tor locus to the third autosome, whereas it was previously thought to be on the first autosome, a finding that will be important for future studies of the locus. We find that the presence of the bacterial symbiont Regiella is associated with higher levels of wing plasticity. Genome‐wide association analysis of wing plasticity variation did not reveal an impact of the tor locus, consistent with independence of body colour and wing plasticity. This analysis implicated one possible candidate gene—a Hox gene, abdominal‐A—underlying wing plasticity variation, although SNPs do not reach the level of genome‐wide significance and therefore will require further study. Our study highlights that plasticity variation is complex, impacted by a bacterial symbiont and genetic variation, but not influenced by body colour.
The Effect of Online Infant Care Education and Postpartum Counseling Based on Meleis's Transition Theory on Mothers' Self‐Esteem and Infant Health: A Randomized Controlled Trial
Authors: Bilgiç Fatma Şule , Bozkurt Gülçin 
Published: February 2025

ABSTRACTThe aim of this research was to assess the effects of online infant care education and postnatal counseling, based on Meleis's transition theory, on maternal self‐esteem and infant health. The randomized controlled trial was conducted between November 2022 and May 2023 with 102 mothers and their infants, n = 52 in the intervention and n = 50 in the control. The intervention group received training across two sessions between 32 and 38 weeks of gestation and counseling support for 3 months postbirth. The control group was shared with additional educational materials in PDF format in addition to their usual care. In the intervention group, the subdimension and total score mean of the Karitane Parental Self‐Confidence Scale during pregnancy, postpartum, first, second, and third months were higher than the control group (p = 0.001). The proportion of exclusive breastfeeding in the third month was higher in the intervention group than in the control group (p = 0.001). Newborn nurses' care on Meleis' transition theory will increase the quality of care and newborn health. In our study, Meleis's transition theory provides a strong foundation for understanding mothers' psychological and physical transitions in the postpartum period.Trial Registration: NCT05812833
Lived Experiences of Turkish Internationally Educated Nurses: A Phenomenological Study
Authors: Gülşen Mehmet , Akan Dilan Deniz , Tosun Salih 
Published: February 2025

ABSTRACTThe international mobility of the nursing workforce is growing. In recent years, Türkiye has seen a notable increase in the number of nurses seeking employment abroad. This study aimed to describe lived experiences of Turkish internationally educated nurses (IENs). A qualitative design with a descriptive phenomenological approach was employed, and the results are reported following the consolidated criteria for reporting qualitative research (COREQ) checklist. Data were collected from September to November 2023 through in‐depth, individual online interviews using a semi‐structured interview form. Sixteen Turkish IENs were interviewed. Through thematic analysis, four themes emerged: push factors of migration, pull factors of migration, positive experiences following migration, and negative experiences following migration. The migration of Turkish nurses to higher‐income countries significantly enhanced their professional and personal lives but also presented considerable challenges. This study underscores the dual nature of migration, offering both opportunities and challenges.
Yoga, Meditation, Mindfulness, or Hypnotherapy for GI Disorders: Similar Mechanisms of Action?
Authors: Hamal Anjal , Shin Andrea , van Tilburg Miranda A. L. 
Published: February 2025

ABSTRACTMind–body approaches aim to improve gut symptoms and quality of life by targeting the interaction between the central nervous system and the enteric nervous system. These include treatments such as hypnotherapy, mindfulness, meditation, and yoga. Although evidence is building on efficacy of mind–body approaches, we generally lack a thorough understanding of how they work. Despite being presented as separate treatment modalities, mind–body approaches often use overlapping treatment aspects with the same mechanism of action. There is evidence that yoga, meditation, and hypnotherapy may partly draw their benefit from creating an absorbed state of attention combined with suggestions for change. This has implications for clinical application of these treatments in patients with GI disease. We propose studies on mechanisms of mind–body approaches to develop more efficacious and more precise treatments for GI diseases.
Memory‐guided perception is shaped by dynamic two‐stage theta‐ and alpha‐mediated retrieval
Authors: Fischer Manda , Moscovitch Morris , Alain Claude 
Published: February 2025

AbstractHow does memory influence auditory perception, and what are the underlying mechanisms that drive these interactions? Most empirical studies on the neural correlates of memory‐guided perception have used static visual tasks, resulting in a bias in the literature that contrasts with recent research highlighting the dynamic nature of memory retrieval. Here, we used electroencephalography to track the retrieval of auditory associative memories in a cue–target paradigm. Participants (N = 64) listened to real‐world soundscapes that were either predictive of an upcoming target tone or nonpredictive. Three key results emerged. First, targets were detected faster when embedded in predictive than in nonpredictive soundscapes (memory‐guided perceptual benefit). Second, changes in theta and alpha power differentiated soundscape contexts that were predictive from nonpredictive contexts at two distinct temporal intervals from soundscape onset (early—950 ms peak for theta and alpha, and late—1650 ms peak for alpha only). Third, early theta activity in the left anterior temporal lobe was correlated with memory‐guided perceptual benefits. Together, these findings underscore the role of distinct neural processes at different time points during associative retrieval. By emphasizing temporal sensitivity and by isolating cue‐related activity, we reveal a two‐stage retrieval mechanism that advances our understanding of how memory influences auditory perception.
Cost effectiveness of fortified bouillon in addressing Burkinabe children's vitamin A inadequacy: An economic optimization model
Authors: Colina Armando R. , Vosti Stephen A. , Jarvis Michael , Engle‐Stone Reina , Michuda Aleksandr , Ortiz‐Becerra Karen , Adams Katherine P. 
Published: February 2025

AbstractVitamin A dietary inadequacy remains a serious public health problem among young children 6–59 months of age in Burkina Faso. Planners face several interrelated challenges: Selecting concrete policy objectives regarding vitamin A inadequacy reductions, identifying cost‐effective vitamin A intervention programs that can achieve those objectives, and being reasonably sure that proposed intervention programs are robust to uncertainty in program benefits and costs. A 10‐year, subnational economic optimization model making use of secondary dietary intake data and program cost data was developed and implemented to address these issues and included the following vitamin A program options: existing or improved edible oils fortification, a pair of hypothetical vitamin A‐fortified bouillon programs, and a set of subnational vitamin A supplementation (VAS) programs. The model consistently identified the improved edible oils and bouillon fortification programs as the core national programs upon which the more expensive subnational VAS programs could be layered, depending on policy objectives and available funding. These results were robust to uncertainty in program nutritional benefits and costs. However, even if the most impactful set of modeled programs was implemented, vitamin A inadequacy among children would remain a serious public health problem; hence, additional efforts to address it would be needed.
Monitoring measurable residual disease in NUP98::NSD1‐positive acute myeloid leukemia
Authors: Okuchi Ikuo , Nishimura Akira , Kamiya Takahiro , Sasaki Makiko , Yamashita Motoi , Hoshino Akihiro , Kajiwara Michiko , Isoda Takeshi , Kanegane Hirokazu , Morio Tomohiro , Takagi Masatoshi 
Published: February 2025

AbstractBackgroundNUP98 fusion genes are detected in acute myeloid leukemia (AML) subgroups that have a poor prognosis. An appropriate therapeutic approach should therefore be established. Treatment intensification according to the minimal residual disease (MRD) level can lead to a better prognosis in patients with acute lymphoblastic leukemia (ALL). However, the importance of MRD monitoring in the patient with NUP98‐positive AML is unclear.MethodsThis study aimed to develop a digital droplet polymerase chain reaction (ddPCR) method for monitoring NUP98::NSD1‐positive leukemic cells and to report its utility compared with the results of NUP98 split fluorescence in situ hybridization (FISH).ResultsThe results of NUP98::NSD1 ddPCR correlated with those of NUP98 split FISH and were more sensitive than NUP98 split FISH. The sensitivity of ddPCR was 0.001%, equivalent to 1 in 1 × 105 cells. The MRD level of NUP98::NSD1, measured by ddPCR, correlated well with relapse.ConclusionThe use of ddPCR to target NUP98::NSD1 chimera mRNA for MRD monitoring would be beneficial for NUP98::NSD1 AML treatment.
Empathic Accuracy Across Childhood, Adolescence, and Within Parent‐Child Interactions: A Systematic Review
Authors: Mauroy Anthony , Galdiolo Sarah , Meillerais Sandie , Verhofstadt Lesley L. , Gaugue Justine 
Published: February 2025

ABSTRACTEmpathic accuracy (EA) is the ability to precisely understand someone else's current thoughts and feelings during an interaction. EA has mostly been studied in adult populations; variations in EA in children/adolescents could be explained by age, developmental changes during childhood, and environmental factors. A systematic review was conducted to synthesize the existing literature on EA in childhood, adolescence, and during parent–child interactions. Following PRISMA guidelines, five electronic databases were searched, yielding 24 references. Most studies were conducted in samples of adolescents. Additionally, the operationalization of EA varied across studies. Although studies showed minor variations of EA with age, there is no clear association of age with EA during childhood. Children and adolescents also achieve similar scores as adults. Additionally, children's and parents' EAs are linked to parents' and children's behaviors during interactions. EA is also associated with gender beliefs: parents and children hold beliefs that girls and mothers should have higher empathy than boys and fathers. It is not possible to determine age variation in EA during childhood. This systematic review highlights a lack of transparent methodologies and specific assessments of EA in children and adolescents in the current literature.
A skin explant model for studying UV‐induced DNA damage and repair
Authors: Payne Hailey , Athans Christina , Wu Shiyong , Bahamondes Lorca Veronica 
Published: February 2025

AbstractThere is a growing need for a skin model that combines the natural physiology of skin while reducing reliance on mice. Natural physiology is achieved by using fresh, intact skin explants sourced from living organisms such as humans or mice. This study focused on the standardization and characterization of an in vitro mouse skin explant model for investigating solar ultraviolet (sUV)‐induced skin damage. We developed a protocol to use skin explants derived from the discarded tissue of mice after euthanasia. These explants consist of intact dermal and epidermal layers suspended in cell culture medium and maintained in vitro. To assess the viability of the skin explants, we evaluated tissue morphology (via hematoxylin and eosin [H&E] staining), viability markers, and DNA damage markers. Our ex vivo model preserves the key characteristics and physiological responses of in vivo skin for short incubation periods, while minimizing the use of mice. This model enables the study of DNA damage and repair, and it has broad applications, including studies on skin photoprotection, topical treatments, drug development, and cosmetics.
A novel acquired resistance mechanism to 5‐aminolevulinic acid‐mediated photodynamic therapy with ABCG2 inhibition
Authors: Chandratre Sharayu , Olsen Jordyn , Chen Bin 
Published: February 2025

AbstractWe report the occurrence of acquired tumor cell resistance to 5‐aminolevulinic acid (ALA)‐mediated photodynamic therapy (PDT) in combination with ABCG2 inhibition. ALA‐PDT in combination with either an ABCG2 tool inhibitor Ko143 or a repurposed clinically‐relevant ABCG2 inhibitor lapatinib was highly effective in eradicating the H4 human glioma cells, resulting in minimal cell survival after treatment. However, after seven rounds of repeated treatments with light dose escalation, the resultant tumor cells became resistant to the combination therapy. The resistant sublines and the parental cell line showed similar ABCG2 activities and protein levels, indicating that it was not ABCG2 that caused the resistance. They also exhibited similar responses to PpIX‐PDT and mTOR inhibitor AZD2014, suggesting that alterations in PDT sensitivity and mTOR pathway had little contribution to the development of resistance phenotype. By determining the intracellular and extracellular PpIX levels, the activities and protein levels of heme biosynthesis enzymes, we found that porphobilinogen deaminase (PBGD) activity and protein level were significantly reduced in the resistant sublines, causing resistance to PDT by substantially reducing PpIX biosynthesis. A novel acquired resistance mechanism to ALA‐PDT with ABCG2 inhibition has been uncovered.
The promotive and repressive effects of exogenous H2O2 on Arabidopsis seed germination and seedling establishment depend on application dose
Authors: Wang Yakong , Liu Xiaohong , Sun Xiangyang , Mao Xiaonan , Wang Zhaoye , Peng Jun , Yang Zuoren , Ali Faiza , Wang Zhi , Li Fuguang 
Published: February 2025

AbstractHydrogen peroxide (H2O2) displays significant and dual effects on seed germination and seedling development, depending on the application dosage. However, the definition of H2O2 thresholds and the mechanisms underlying the dual actions in Arabidopsis seed germination and seedling development are not yet clear. Here, we analyzed the Arabidopsis seed germination profiles in response to different concentrations of exogenous H2O2 and found that 2 mM functions as the key threshold, above this threshold, both seed germination and seedling establishment were gradually inhibited. By RNA‐seq analysis and function verification, we identified pathways of abscisic acid (ABA) signalling, seed post‐ripening, energy metabolism, ROS homeostasis, and cell wall loosening play positive roles in seed germination and seedling establishment downstream of the H2O2 signalling. Further physio‐chemical approaches revealed that exogenous H2O2 affected the accumulation and distribution of O2•‐ and H2O2 in embryonic tissues by regulating the tissue‐specific expression of SDH2‐3, RHD2, and PRXs. Collectively, we found that germination rate and aerial growth were positively correlated with endogenous H2O2 content and root length was positively correlated with O2•‐ accumulation, demonstrating that different ROS signals played specific functions in different tissues and development processes. On the other hand, excessive H2O2 (10 mM) represses these two processes for radicle cell damage caused by oxidation stress. Finally, we put forward the mechanism model of the dual effects of exogenous H2O2 on seed germination and seedling establishment.
LDL2 and PAO5 genes are essential for systemic acquired resistance in Arabidopsis thaliana
Authors: Saxena Shobhita , Roy Shweta , Ahmad Mir Nasir , Nandi Ashis Kumar 
Published: February 2025

AbstractA partly infected plant becomes more resistant to subsequent infections by developing systemic acquired resistance (SAR). Primary infected tissues produce signals that travel to systemic tissues for SAR‐associated priming of defense‐related genes. The mechanism through which mobile signals contribute to long‐lasting infection memory is mostly unknown. RSI1/FLD, a putative histone demethylase, is required for developing SAR. Here, we report that two other FLD homologs, LSD1‐LIKE2 (LDL2) and POLYAMINE OXIDASE 5 (PAO5), are required for SAR development. The mutants of LDL2 and PAO5 are not defective in local resistance but are specifically impaired for SAR. The mutants are defective in salicylic acid accumulation and priming of defence‐related genes such as PR1, FMO1, and SnRK2.8. LDL2 and PAO5 are expressed in systemic tissues upon SAR induction by pathogens or SAR mobile signal azelaic acid. The ldl2 and pao5 mutants generate SAR mobile signals like wild‐type (WT) plants but fail to respond to the signal at the systemic leaves. Both LDL2 and PAO5 proteins contain polyamine oxidase (PAO) domains, suggesting their involvement in polyamine metabolism. Exogenous applications of polyamines such as spermine and spermidine activate SAR in WT and rescue SAR defects of ldl2 and pao5 plants. Inhibition of polyamine biosynthetic gene arginine decarboxylase blocks SAR development. Results altogether demonstrate specific non‐redundant roles of LDL2 and PAO5 in SAR development with their possible involvement in polyamine metabolism.
Anti‐Sea Lice Veterinary Medicinal Products on Salmon Farms: A Review and Analysis of Their Usage Patterns, Environmental Fate and Hazard Potential
Authors: Parsons Aoife E. , Escobar‐Lux Rosa H. , Hannisdal Rita , Agnalt Ann‐Lisbeth , Samuelsen Ole B. 
Published: February 2025

ABSTRACTAquaculture is expanding globally and the environmental implications associated with this growth, including the increasing use of chemicals in aquatic locations, are an emerging concern for environmental legislators. Veterinary medicinal products (VMP) are used as chemotherapeutants to control sea lice infestations on open‐net pen salmon farms and questions have been raised about their potential to cause adverse effects on marine ecosystems. The current study analyses the usage patterns of anti‐sea lice VMPs in major salmonid aquaculture regions and reviews the current knowledge on their mode of action, routes into the environment and environmental fate. We subsequently evaluate their hazard potential by performing a toxicity assessment and deriving up‐to‐date preliminary environmental quality standards (EQS). We demonstrate that the use of anti‐sea lice VMPs varies between the major salmon‐producing countries, with quantities varying over time and depending on the chemical. Norway and Chile use the highest quantity of chemical treatments, consistent with their higher salmon production capacity, and while the reliance on some chemicals has decreased the use of newly authorised chemicals has increased. Our toxicity assessment revealed that there was sufficient reliable acute toxicity data to derive EQS for five of the selected anti‐sea lice VMPs using the probabilistic method of constructing species sensitivities distribution curves. However, insufficient chronic and sediment toxicity data were available for all chemicals and therefore EQS were derived using the traditional deterministic method. The information presented in this review can guide future research and facilitate better environmental risk assessments of chemotherapeutant used on salmon farms.
Dynapenia and Sarcopenia as Risk Factors for Mortality in Interstitial Lung Disease
Authors: Ibarra‐Fernández Alan Aldair , Robles‐Hernández Robinson , Orea‐Tejeda Arturo , González‐Islas Dulce , Jiménez‐Valentín Angelia , Sánchez‐Santillán Rocío , Arcos‐Pacheco Laura Patricia , Gutiérrez‐Luna Emilio , Zurita‐Sandoval Andrea , Peña‐Espinosa Tomas , Gutiérrez‐Vargas Rosaura , Flores‐Cisneros Laura 
Published: February 2025

ABSTRACTBackground and ObjectiveFibrotic interstitial lung disease (ILD) is associated with high morbidity and mortality. Patients often exhibit impaired nutritional status and alterations in body composition, such as dynapenia and sarcopenia, which correlate with poor pulmonary function, reduced exercise tolerance and diminished quality of life. However, the impact of dynapenia and sarcopenia on prognosis has not been examined extensively in ILD patients.We assessed the impact of dynapenia and sarcopenia as risk factors for mortality and their prevalence in ILD.MethodsProspective cohort study. ILD was classified into idiopathic pulmonary fibrosis (IPF), connective tissue disease‐related ILD (CTD‐ILD) and chronic hypersensitivity pneumonitis (CHP). Patients over 18 years old with a confirmed diagnosis of ILD were included, while those with diagnoses of cancer, human immunodeficiency virus and neurological disease were excluded. Dynapenia and sarcopenia were determined according to EWGSOP2 criteria.ResultsNinety‐eight ILD patients were included; 33.66% had IPF, 47.96% had CTD‐ILD, and 18.37% had CHP. The mean age was 63.89 ± 12.02 years; 37.76% were male.The risk factors associated with mortality included dynapenia (HR: 2.04, 95% CI: 1.10–3.77, p = 0.022), sarcopenia (HR: 1.88, 95% CI; 1.00–3.33, p = 0.049) and exercise tolerance (HR: 0.99, 95% CI; 0.99–0.99, p = 0.023), adjusted for confounding variables.The prevalence of dynapenia was 45% in ILD; 51% in IPF, 35% in CTD‐ILD and 61% in CHP. The prevalence of sarcopenia was 29%; both IPF (39%) and CHP (50%) had a higher prevalence of sarcopenia than CTD‐ILD (14%).ConclusionSarcopenia and dynapenia are independent risk factors for mortality in ILD.
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