bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amelioration of premature aging in Werner syndrome stem cells by targeting SHIP/AKT pathway
Authors: Tam Hei-Yin , Liu Jiaxing , Yiu Tsz-Ching , Leung Adrian On-Wah , Li Chang , Gu Shen , Rennert Owen , Huang Boxian , Cheung Hoi-Hung 
Published: January 2025

Abstract

Background
Pathogenic or null mutations in WRN helicase is a cause of premature aging disease Werner syndrome (WS). WRN is known to protect somatic cells including adult stem cells from premature senescence. Loss of WRN in mesenchymal stem cells (MSCs) not only drives the cells to premature senescence but also significantly impairs the function of the stem cells in tissue repair or regeneration.

Results
In this study, we profiled the signaling pathways altered in WRN-deficient MSC and applied pharmacological method to activate the AKT signaling in these cells and examined their cellular phenotype related to aging. We found that the AKT signaling in WRN-deficient MSCs was significantly suppressed while the AKT upstream phosphatases (SHIP1/2) were upregulated. Knockdown or inhibition of SHIP1/2 could ameliorate premature senescence in WRN-deficient MSCs. Moreover, SHIP inhibition stimulated MSC proliferation and suppressed expression of pro-inflammatory cytokines IL-6 and IL-8. The stemness of WRN-deficient MSC was also improved upon pharmacological treatments with the inhibitors.

Conclusions
These results suggested that targeting the SHIP/AKT signaling pathway is beneficial to WRN-deficient stem cells and fibroblasts, which might be applied for improving the trophic function of MSC in, for instance, promoting angiogenesis.
Syndemic, mental health and living with dependent persons in Latin America and Spain: a study with a gender perspective
Authors: López-Contreras Natalia , López-Jiménez Tomás , Medina-Perucha Laura , León-Gómez Brenda Biaani , Gonçalves Alessandra Queiroga , Horna-Campos Olivia Janett , Anigstein Maria Sol , Barbosa Jakeline Ribeiro , Verotti Mariana Pastorello , Bardales-Mendoza Olga , Arteaga-Contreras Karen M. , Berenguera Anna , Peralta Andrés , Jacques-Aviñó Constanza 
Published: January 2025

Abstract

Objective
To analyze the sociostructural determinants associated with mental health problems during the lockdown period among populations residing in Brazil, Chile, Ecuador, Mexico, Peru, and Spain who lived with minors or dependents, approached from a gender perspective.

Methods
A cross-sectional study was conducted in six participating countries via an adapted, self-managed online survey. People living with minors and/or dependents were selected. Multivariate logistic regression models were estimated to assess the associations between sociostructural variables and mental health problems (anxiety (GAD-7) and/or depression (PHQ-9)). The analyses were stratified by sex and country.

Results
Out of a total of 39,006 people, 18,040 reported living with minors and/or dependents (73% women). In all countries, women reported worse mental health, with Spain having a lower prevalence. The risks of mental health problems in women in most countries are associated with poor housing conditions and performing care work. University education was associated with a protective factor. For men, risks were related to being younger, worsening working conditions and concerns about living together at home.

Conclusions
Women in Latin America who lived with dependents had worse outcomes than those in Spain did. It is necessary to develop intersectoral and social determinants strategies to prevent, protect and support the mental health of those who live with dependents and minors.
Increased matrix metalloproteinase-1 expression by coexposure to UVA and cigarette sidestream smoke and contribution of histone acetylation
Authors: Ito Ryoma , Komaki Yukako , Ibuki Yuko 
Published: January 2025

Abstract

Background
Skin is exposed to various environmental factors throughout life, and some of these factors are known to contribute to skin aging. Long-term solar UV exposure is a well-known cause of skin aging, as is cigarette smoke, which contains a number of chemicals. In this study, combined effect of UVA and cigarette sidestream smoke (CSS) on matrix metalloproteinase-1 (MMP-1) induction was investigated. MMP-1 is the main protease that initiates collagen type I fiber fragmentation in human skin and is associated with aging.

Results
Combined exposure to UVA and CSS enhanced MMP-1 induction, accompanied by collagen type I (COL1A1) gene suppression. The basal expression of MMP-1 was higher in senescent cells than in normal cells, with a pronounced increase after coexposure to UVA and CSS. UVA irradiation resulted in global histone H3 acetylation, and we considered this was responsible for the MMP-1 upregulation. Histone deacetylase inhibitors, sodium acetate, propionate, and butyrate, all enhanced the CSS-induced MMP-1 according to the degree of histone acetylation.

Conclusion
These results suggest that UVA and CSS additively induce MMP-1, which may lead to skin aging, and that such combined effect may further promote aging in aged skin. UVA-induced histone acetylation may contribute to MMP-1 induction.
METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling
Authors: Pu Mei , Xiao Xia , Lv Shasha , Ran Daqing , Huang Qian , Zhou Mingming , Lei Qirong , Kong Lingshuang , Zhang Qing 
Published: January 2025

Abstract

Background
Discs large homolog 2 (DLG2) has been implicated in cancer development, yet its role in cervical cancer remains unclear. This study aims to explore the regulatory mechanism of DLG2 in cervical cancer and its clinical implications.

Methods
Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. Colony formation assay, 5-Ethynyl-2’-deoxyuridine assay, flow cytometry, and transwell assays were conducted for cell functional analysis. A xenograft mouse model assay was performed to analyze tumor tumorigenesis in vivo. m6A RNA immunoprecipitation assay was used to analyze the association of METTL3 and DLG2.

Results
DLG2 was underexpressed in cervical cancer tissues and cells. Elevating DLG2 levels significantly suppressed cervical cancer cell proliferation, migration, and invasion, while promoting apoptosis. Additionally, DLG2 overexpression led to the deactivation of the Hippo/YAP signaling pathway. In vivo, DLG2 overexpression was shown to reduce tumor formation. We also discovered that METTL3 destabilized DLG2 mRNA through an m6A-dependent mechanism. Moreover, lowering DLG2 expression mitigated the effects of METTL3 silencing on cervical cancer cell malignancy.

Conclusion
DLG2 acted as a tumor suppressor in cervical cancer by inhibiting the Hippo/YAP signaling pathway. The METTL3-dependent regulation of DLG2 mRNA stability could be a critical factor in cervical cancer progression.
Exploring the bidirectional causality between neuroticism and frailty: a Mendelian randomization analysis
Authors: Xing Yuhang , Pu Rui , Fu Mengdie , Wang Zhikang , Wang Zhen , Shang Xiaopeng , Yang Guoli , Jiang Zhiwei 
Published: January 2025

Abstract

Background
Epidemiological studies have confirmed the relationship between personality trait neuroticism and physical health. However, the relationship between neuroticism and frailty remains unconfirmed. This study employed a bi-directional two-sample Mendelian randomization (MR) approach to investigate the causal relationship between neuroticism and frailty.

Methods
The neuroticism genome-wide association study (GWAS) data from the UK Biobank contained twelve neuroticism-related traits with 489,212 participants. The genetic frailty index data were extracted from the UK Biobank and Swedish TwinGene, involving 175,226 individuals. Independent genetic variants associated with neuroticism and frailty were selected as instrumental variables. Inverse variance weighted (IVW), MR-Egger, weighted median, weighted mode, and MR-PRESSO were mainly used for MR analysis.

Results
The MR analysis showed a positive causal relationship between neuroticism and the risk of frailty (odds ratio (OR) = 1.627, 95% confidence interval (CI) = 1.538–1.722, P < 0.001). In the reverse direction, frailty had a causal effect on a higher risk of neuroticism (OR = 1.270, 95% CI = 1.173–1.375, P < 0.001). Steiger tests indicated that reverse causation did not bias the identified causal relationships.

Conclusions
Our study provides genetic evidence suggesting a bi-directional causal relationship between frailty and neuroticism. In this bi-directional MR study, there were positive causal relationships between neuroticism-related phenotypes and frailty, and in the reverse direction, frailty was also positively correlated with neuroticism.
Altered medial temporal lobe subregion volumes in systemic lupus erythematosus patients with neuropsychiatric symptoms
Authors: Makdad Najeeb Z. , Sundgren P. C. , Jönsen A. , Zervides K. , Lätt J. , Salomonsson T. , Nystedt J. , Nilsson P. , Bengtsson A. , Kuchcinski G. , Wisse L. E. M. 
Published: January 2025

Abstract

Background
Systemic lupus erythematosus (SLE) often presents with neuropsychiatric (NP) involvement, including cognitive impairment and depression. Past magnetic resonance imaging (MRI) research in SLE patients showed smaller hippocampal volumes but did not investigate other medial temporal lobe (MTL) regions. Our study aims to compare MTL subregional volumes in SLE patients to healthy individuals (HI) and explore MTL subregional volumes in relation to neuropsychiatric SLE (NPSLE) manifestations.

Methods
A total of 70 SLE patients and 25 HI underwent clinical evaluation, cognitive testing, and 3 tesla MRI imaging. T1-weighted MRI images were analyzed using the Automatic Segmentation of Hippocampal Subfields-T1 software. Analyses of Covariance were used to compare MTL subregion volumes between SLE and HI, and between NPSLE and non-NPSLE patients according to three models: the American College of Rheumatology (ACR) model defined by the ACR case definitions for NPSLE (n = 42), the more stringent Systemic Lupus International Collaborating Clinics (SLICC) B model (n = 21), and the most stringent SLICC A model (n = 15). Additionally, we explored the relation between MTL subregion volumes, cognitive functions, and depression scores in SLE patients using partial correlation analyses.

Results
Significantly smaller volumes of bilateral whole hippocampus, anterior hippocampus, posterior hippocampus, and Brodmann Area 35 were demonstrated in NPSLE compared to non-NPSLE patients according to the ACR model (p = 0.01, p = 0.03, p = 0.04, and p = 0.01 respectively). The differences did not reach significance according to the SLICC B and SLICC A models. No significant differences in MTL subregional volumes between SLE patients and HI were found. Partial correlation analyses showed a significant positive correlation between left Brodmann Area 35 volume and complex attention scores in SLE patients. No significant associations between MTL subregion volumes and depression scores were demonstrated.

Conclusions
NPSLE patients display significantly smaller volumes in various subregions of the MTL compared to non-NPSLE patients. These findings are suggestive of neuronal damage in MTL subregions in NPSLE patients on a group level.
Advancing soil-structure interaction (SSI): a comprehensive review of current practices, challenges, and future directions
Authors: Najar Imtiyaz Akbar , Ahmadi Raudhah , Amuda Akeem Gbenga , Mourad Raghad , Bendary Neveen El , Ismail Idawati , Bakar Nabilah Abu , Tang Shanshan 
Published: January 2025

Abstract
The safety, stability, and long-term performance of reinforced concrete (RC) structures depend significantly on soil-structure interaction (SSI), a critical phenomenon governing the dynamic relationship between soil and structural behaviour. SSI plays a pivotal role in seismic design, influencing the stiffness, damping, and natural frequency of structures, yet its application in practical design remains underutilized due to challenges in modelling and integrating code provisions. This review synthesizes existing knowledge on SSI, emphasizing its impact on buildings, bridges, and foundations under static and dynamic loads. It highlights advancements in analytical, numerical, and experimental modelling methods, such as finite element analysis and discrete element methods, and evaluates their effectiveness in capturing the complex interactions between soil and structural systems. The review identifies key gaps, including a lack of unified guidelines in international codes, inadequate integration of SSI in real-world design processes, and limited exploration of its role in emerging engineering challenges like sustainability and climate resilience. Historical seismic events, such as the Kobe and Loma Prieta earthquakes, are analysed to underscore the detrimental consequences of neglecting SSI considerations. Additionally, the review discusses recent innovations, including the application of machine learning and advanced computational tools, and their potential to enhance the accuracy and efficiency of SSI analysis. This study offers actionable insights for improving design practices, such as adapting SSI frameworks for structures on soft soils and incorporating dynamic interactions in seismic design codes. It concludes with a call for interdisciplinary collaboration and future research into novel SSI applications, including its integration with smart sensing technologies and sustainable infrastructure design. This review bridges the gap between theoretical advancements and practical applications of soil-structure interaction (SSI) by synthesizing current knowledge, identifying critical research gaps, and proposing innovative solutions to enhance structural resilience, sustainability, and seismic safety.

Graphical Abstract
Infection with hepatitis C virus as a cause of cirrhosis – case study
Authors: Sierpińska Lidia , Januszek Małgorzata 
Published: January 2025

Background and study aims. HCV may cause an acute or chronic hepatitis C and, when left untreated, may lead to cirrhosis and/or primary liver cancer. The aim of the study was analysis of personal medical records of a patient with cirrhosis from the aspect of its relation with HCV infection;
Materials and Method. A case of a 36-year old patient was presented diagnosed with cirrhosis, admitted to the emergency department due to haemorrhage from the upper gastrointestinal tract. Anti-HCV antibodies were present. The method was a case study, the technique – analysis of records the research tool – case history with the results of diagnostic tests, visual analogue scale (VAS) measuring pain intensity, the Hamilton Depression Rating Scale, fluid balance chart, and an author-constructed questionnaire to be completed by the patient;
Results. Based on internal documentation of the hospital and patient medical records a case of a patient was described with gastrointestinal bleeding and cirrhosis, diagnosed with HCV. Physical examination was presented, as well as epidemiological interview, medical history, course of diagnostics and treatment of the patient qualified for follow up ambulatory care in the outpatient hepatology department.
Conclusions. Screening tests are recommended in order to early diagnose the presence of anti-HCV antibodies. Early diagnosis of infection with HCV and implementation of anti-viral treatment may prevent cirrhosis of the liver. Health education of society is necessary from the aspect of risk factors of HCV infection in the medical environment, as well as in beauty salons and tattoo parlours.
Working effectively with individuals with substance use disorders: a qualitative descriptive study
Authors: Tamayo Niall , Lane Annette , Petrovic Kristin , Moghabghab Rola 
Published: January 2025

Background: Irrespective of where nurses work, they may provide care to individuals with substance use disorders. Unfortunately, some nurses may not understand how to work with these individuals and stigmatisation may occur. Aim: To explore how to provide effective care for these individuals. Method: A qualitative descriptive study examining helpful interventions for individuals with substance use disorders through interviewing four substance abuse specialist nurses. Findings: Findings consider person-centred care, such as how to create a therapeutic relationship with individuals with substance use disorders, empowerment of these individuals and providing hope, and employing a holistic approach. Conclusion: Nurses may encounter individuals with substance use disorders and may benefit from knowledge of how best to intervene.
Association of Geriatric Nutritional Risk Index Scores with Outcomes in Patients Undergoing Maintenance Hemodialysis
Authors: Yang Yuting , Yang Yujuan , Hu Yanping , Shi Xintian , Huang Jiayan , Wan Junhui , He Hong , Chen Qinkai , Wang Yu , Zhao Qing 
Published: January 2025

Aims/Background The Geriatric Nutritional Risk Index (GNRI) is an effective tool for identifying malnutrition, and helps monitor the prognosis of patients undergoing maintenance hemodialysis. However, the association between the GNRI and cardiovascular or all-cause mortality in hemodialysis patients remains unclear. Therefore, this study investigated the correlation of the GNRI with all-cause and cardiovascular mortality in patients undergoing maintenance hemodialysis. Methods This study included 402 incident hemodialysis patients. Based on the first-dialysis GNRI quartile, the patients were divided into four groups: N1 (GNRI ≤85.04), N2 (GNRI 85.05–91.04), N3 (91.05–96.49), N4 (GNRI ≥96.5) groups. The risk of all-cause and cardiovascular mortality among the different GNRI groups was compared using the Kaplan-Meier survival curve analysis through log-rank tests. Furthermore, Cox regression analysis was used to assess the association between the GNRI groups and all-cause mortality. Additionally, the predictive capability of the GNRI groups on the prognosis was evaluated by employing receiver operating characteristic (ROC) curve analysis. Results Patients in the N1 group exhibited a significantly higher risk of all-cause mortality (p < 0.001) and cardiovascular mortality (p = 0.004) compared to the other groups. ROC curve analysis revealed that GNRI, age, and serum creatinine had moderate predictive value for mortality, with GNRI indicating an area under the curve (AUC) of 0.605 for all-cause mortality and 0.565 for cardiovascular mortality. Moreover, the N2 and N3 groups had a significantly reduced risk of cardiovascular mortality compared to the N1 group. Conclusion A lower GNRI is closely associated with a higher risk of all-cause and cardiovascular mortality in hemodialysis patients.
Study of the cone of sums of squares plus sums of nonnegative circuit forms
Authors: Dressler Mareike , Kuhlmann Salma , Schick Moritz 
Published: January 2025

Abstract
In this article, we combine sums of squares (SOS) and sums of nonnegative circuit (SONC) forms, two independent nonnegativity certificates for real homogeneous polynomials. We consider the convex cone SOS+SONC of forms that decompose into a sum of an SOS and a SONC form and study it from a geometric point of view. We show that the SOS+SONC cone is proper and neither closed under multiplication nor under linear transformation of variables. Moreover, we present an alternative proof of an analog of Hilbert’s 1888 Theorem for the SOS+SONC cone and prove that in the non-Hilbert cases it provides a proper superset of the union of the SOS and SONC cones. This follows by exploiting a new necessary condition for membership in the SONC cone.
ESTRATEGIA DEL EQUIPO DE BALONMANO 13-15 AÑOS MASCULINO DE GRANMA A LOS JUEGOS ESCOLARES NACIONALES DE 2024
Authors: Vanega Román Lázaro Anne , Hidalgo Reyes Pedro , Fernández Blez Ramón Nonnato 
Published: January 2025

Las estrategias tienen un uso generalizado en el ámbito empresarial y deportivo. En la presente investigación se empleó esta técnica con el objetivo de contribuir a un adecuado desempeño del equipo de balonmano escolar 13-15 años, masculino, de la provincia Granma, en Cuba. El equipo que tiene como principal competencia, la edición 60 de los Juegos Escolares Nacionales, a desarrollarse en julio de 2024. Durante la investigación fue necesario el uso de métodos de nivel teórico y empírico. De los teóricos, el analítico-sintético y entre los empíricos, el análisis de documentos y la observación, resultaron vitales. Se emplearon además, técnicas de consenso y generación de ideas. La estrategia la integraron como principales componentes: el Análisis del ciclo anterior, el Diagnóstico estratégico, la Determinación de los objetivos (generales, específicos, del periodo competitivo y de la etapa de preparación física especial), Opciones estratégicas y Plan de entrenamiento. La estrategia tuvo como principales soportes, la gestión estratégica y el entrenamiento deportivo. Esta constituye una alternativa de dirección válida para alcanzar resultados competitivos destacados en el equipo deportivo objeto de estudio.
IMPLEMENTACIÓN DE UN PROGRAMA DE AF PARA PROMOVER HABILIDADES SOCIALES EN ESTUDIANTES DE SECUNDARIA EN EL ARCHIPIÉLAGO DE SAN BERNARDO, BOLIVAR
Authors: Rodríguez Giraldo Airinth Andrea , Mantilla Sonia Carolina 
Published: January 2025

Este estudio evalúa el impacto de un programa de actividad física “Actívate Juventud” en el desarrollo de habilidades sociales en estudiantes de secundaria de una institución etnoeducativa en el Archipiélago de San Bernardo. La investigación responde a las necesidades del contexto local, donde los estudiantes enfrentan retos significativos en cuanto a su desarrollo socioemocional y la interacción interpersonal. El programa, con una duración de 12 semanas, tuvo como objetivo mejorar las habilidades de los actividades físicas estructuradas. La metodología utilizada fue un diseño cuasiexperimental con pretest y postest, en el que participaron 39 estudiantes divididos en grupos de control e intervención. El grupo de intervención participó en actividades físicas dinámicas orientadas a fomentar el trabajo en equipo, la cooperación y la comunicación. Para la evaluación, se utilizó la Escala de Habilidades Sociales (EHS) de Gismero, que mide dimensiones clave como la autoexpresión, la defensa de los derechos como consumidor, la expresión de desacuerdos y la iniciación de interacciones positivas. Los resultados, analizados mediante el software IBM SPSS, mostraron mejoras significativas en las habilidades sociales del grupo de intervención en comparación con el grupo de control. En particular, los estudiantes demostraron una mejor comunicación y una mayor capacidad para interactuar de manera efectiva en contextos interpersonales. El estudio concluyó que la actividad física no solo mejora el bienestar físico de los estudiantes, sino que también contribuye de manera significativa al desarrollo de habilidades sociales esenciales para su desarrollo personal.