bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reliability of transcranial magnetic stimulation-evoked responses on knee extensor muscles during cycling
Authors: Zhang Jenny , McClean Zachary J. , Khaledi Neda , Morgan Sophie-Jayne , Millet Guillaume Y. , Aboodarda Saied Jalal 
Published: February 2024

ABSTRACTTranscranial magnetic stimulation (TMS) measures the excitability and inhibition of corticomotor networks. Despite its task-specificity, few studies have used TMS during dynamic movements and the reliability of TMS-derived measures has not been assessed during cycling. This study aimed to evaluate the reliability of motor evoked potentials (MEP) and short- and long-interval intracortical inhibition (SICI and LICI) on vastus lateralis and rectus femoris muscle activity during a fatiguing single-leg cycling task. Nine healthy adults (2 females) performed two identical sessions of counterweighted single-leg cycling at 60% peak power output until failure. Five single-pulses and five short- and long-interval paired pulses delivered to the motor cortex, and two maximal femoral nerve stimulations [maximal M-wave (Mmax)], were delivered during two baseline cycling bouts (unfatigued) and every 5 min throughout cycling (fatigued). When comparing both baseline bouts within the same session, MEP·Mmax-1and LICI (both ICC: >0.9) were rated excellent while SICI was rated good (ICC: 0.7-0.9). At baseline between sessions, in the vastus lateralis, Mmax(ICC: >0.9) and MEP·Mmax-1(ICC: 0.7) demonstrated good reliability, LICI was moderate (ICC: 0.5), and SICI was poor (ICC: 0.3). Across the fatiguing task, Mmaxdemonstrated excellent reliability (ICC >0.8), MEP·Mmax-1ranged good to excellent (ICC: 0.7-0.9), LICI was moderate to excellent (ICC: 0.5-0.9), and SICI remained poorly reliable (ICC: 0.3-0.6). Overall, these results corroborate the cruciality of retaining mode-specific testing measurements and suggest that during cycling, Mmax, MEP·Mmax-1, and LICI measures are reliable whereas SICI, although less reliable across days, can be reliable within the same session.
ROS responsive Aux/IAA multimerization modulates auxin responses
Authors: Roy Dipan , Mehra Poonam , Mukkawar Vaishnavi , Clark Lisa , Bellande Kevin , Vermeer Joop EM , Arevallilo Raquel Martin , Vernoux Teva , Sue ob Kawinnat , Jones Andrew , Bechtold Ulrike , Mullineaux Phil , Lilley Kathryn , Brown Adrian , Bennett Malcolm , Sadanandom Ari 
Published: February 2024

AbstractReactive oxygen species (ROS) function as key signals in plants to enable adaptation to environmental stresses. Plant roots respond to transient water stress by temporarily ceasing branching using the acclimative response xerobranching1. In this study, we report that a rapid ROS burst regulates Xerobranching by inducing multimerization of auxin repressor protein IAA3/SHY2. Mutations in specific cysteine residues in IAA3/SHY2 disrupt redox-mediated multimerization and interaction with co-repressor TPL, but not with auxin response partner ARF7 and auxin receptor TIR1. ROS-mediated oligomerization of IAA3/SHY2 is required for efficient ARF mediated target gene repression during Xerobranching and lateral root emergence. We demonstrate that AUX/IAA proteins vary in their redox mediated multimerization, revealing a new auxin response regulatory mechanism that directly connects ROS sensing to auxin signalling. Our study reveals how ROS, auxin and water stress intersect to shape acclimative responses in plant roots and maintain their phenotypic plasticity.
Hybrid diffuse optical appraisal of peripheral and cerebral changes in critically ill patients receiving red blood cell transfusion
Authors: Tagliabue Susanna , Rey-Perez Anna , Esposito Lourdes , Jimenez Andrés F. , Angulo Sara Valles , Maruccia Federica , Fischer Jonas B. , Kacprzak Michal , Poca Maria A. , Durduran Turgut 
Published: February 2024

AbstractBackgroundRed blood cells transfusions (RBCT) are utilized to restore normal values of hemoglobin concentration and hematocrit percentage in anemic patients. As expected, RBCT often leads to local and global alteration of blood flow (BF) and blood/tissue oxygenation which could have local deleterious consequences. This complicates its use and its dosage and there is no consensus on liberal versus restrictive RBCT in critically ill patients. Blood gas sampling is utilized to bring objectivity to RBCT which is a reliable systemic measure. However, it is also hypothesized that the knowledge about the dynamic response of selected organs could improve RBCT outcomes. We carried out a study using non-invasive hybrid diffuse optics (DO) to assess the RBCT effect on the brain and a peripheral muscle by evaluating microvascular BF, oxygen extraction fraction (OEF) and microvascular oxy-, deoxy- and total hemoglobin concentrations ([HbO2], [Hhb], [HbT]) in critically ill patients. We explored the DO’s ability to identify RBCT-induced significant alterations and to provide a quantitative description.MethodsCritically ill anemic patients undergoing RBCT were recruited and monitored by hybrid DO. Blood gas samples were extracted to obtain arterial total hemoglobin concentration (Hgb) and hematocrit value. Optical signals, such as BF, OEF, metabolic rate of oxygen extraction (MRO2), [HbO2], [Hhb] and [HbT] were simultaneously measured at the cerebral and the peripheral tissues. The changes in these variables were investigated characterizing the distributions of the cerebral and of the peripheral post-RBCT variables.ResultsFourteen out of fifteen recruited subjects were included. After RBCT, Hgb and hematocrit significantly increased (p<0.001). OEF significantly decreased both at peripheral and cerebral level (p<0.001, p<0.001). A significant increase was found in MRO2(p=0.03, p<0.001), [HbT] (p=0.01, p<0.0001) and [HbO2] (p=0.008, p<0.0001) at both levels. BF significantly decreased only at the peripheral level (p<0.001). No change was encountered in [Hhb] (p>0.05). No statistical difference was found between cerebral and peripheral signals post-RBCT (p>0.05) apart from MRO2(p=0.03, higher at peripheral tissue).ConclusionsHybrid DO detected tissue oxygenation improvement after RBCT, enabling a thorough examination. The potential for DO to quantify and alert changes of concern deserves further investigation.
Mild HIV-specific selective forces overlaying natural CD4+ T cell dynamics explain the clonality and decay dynamics of HIV reservoir cells
Authors: Reeves Daniel B. , Rigau Danielle N. , Romero Arianna , Zhang Hao , Simonetti Francesco R. , Varriale Joseph , Hoh Rebecca , Zhang Li , Smith Kellie N. , Montaner Luis J. , Rubin Leah H. , Gange Stephen J. , Roan Nadia R. , Tien Phyllis C. , Margolick Joseph B. , Peluso Michael J. , Deeks Steven G. , Schiffer Joshua T. , Siliciano Janet D. , Siliciano Robert F. , Antar Annukka A. R. 
Published: February 2024

SummaryThe latent reservoir of HIV persists for decades in people living with HIV (PWH) on antiretroviral therapy (ART). To determine if persistence arises from the natural dynamics of memory CD4+ T cells harboring HIV, we compared the clonal dynamics of HIV proviruses to that of memory CD4+ T cell receptors (TCRβ) from the same PWH and from HIV-seronegative people. We show that clonal dominance of HIV proviruses and antigen-specific CD4+ T cells are similar but that the field’s understanding of the persistence of the less clonally dominant reservoir is significantly limited by undersampling. We demonstrate that increasing reservoir clonality over time and differential decay of intact and defective proviruses cannot be explained by mCD4+ T cell kinetics alone. Finally, we develop a stochastic model of TCRβ and proviruses that recapitulates experimental observations and suggests that HIV-specific negative selection mediates approximately 6% of intact and 2% of defective proviral clearance. Thus, HIV persistence is mostly, but not entirely, driven by natural mCD4+ T cell kinetics.
SbPIP2Mediated Improvements in Plant Resilience: Physiological and Molecular Insights into Abiotic Stress Response
Authors: Patel Jaykumar , Khatri Kusum , Gupta Nirmala Kumari , Maniar Jalak , Khandwal Deepesh , Choudhary Babita , Phillips Dylan Wyn , Jones Huw Dylan , Mishra Avinash 
Published: February 2024

AbstractUnderstanding the mechanisms behind plant resilience to abiotic stresses is essential for enhancing crop yield and sustainability. This study integrates findings from a comprehensive investigation into the function of theSbPIP2gene, which encodes an aquaporin protein, in improving the abiotic stress tolerance of transgenic plants. Our integrated approach revealed that transgenic plants overexpressingSbPIP2significantly reduce reactive oxygen species (ROS) accumulation and exhibit enhanced physiological attributes, including higher seed germination rates, improved growth, early flowering, and better seed setting under stress conditions. Notably, these plants also showed a quicker recovery and completion of their lifecycle post-stress treatment. The transcriptomic analysis provided a deeper understanding of the genetic modifications contributing to stress resilience, highlighting the involvement of genes associated with oxidative stress response, calcium and sugar signaling pathways, stomatal regulation, phytohormone biosynthesis, and flower development. Additionally, the study underscores the central role of abscisic acid (ABA) in mediating stress responses through hormonal regulation, with transgenic plants displaying increased ABA levels due to the upregulation of biosynthesis genes and downregulation of catabolism genes. This hormonal adjustment is critical for stomatal closure, reducing water loss, and enhancing tolerance to abiotic stresses. Our findings elucidate the complex genetic and molecular pathways that underpin abiotic stress tolerance in plants, offering valuable insights for future research aimed at improving crop resilience through genetic engineering, thereby addressing the challenges of climate change and environmental stressors.
Complex carbohydrate utilization by gut bacteria modulates host food preference
Authors: Yu Kristie B. , Son Celine , Chandra Anisha , Paramo Jorge , Novoselov Anna , Özcan Ezgi , Kazmi Sabeen A. , Lum Gregory R. , Lopez-Romero Arlene , Lynch Jonathan B. , Hsiao Elaine Y. 
Published: February 2024

AbstractThe gut microbiota interacts directly with dietary nutrients and has the ability to modify host feeding behavior, but the underlying mechanisms remain poorly understood. Select gut bacteria digest complex carbohydrates that are non-digestible by the host and liberate metabolites that serve as additional energy sources and pleiotropic signaling molecules. Here we use a gnotobiotic mouse model to examine how differential fructose polysaccharide metabolism by commensal gut bacteria influences host preference for diets containing these carbohydrates.Bacteroides thetaiotaomicronandBacteroides ovatusselectively ferment fructans with different glycosidic linkages:B. thetaiotaomicronferments levan with β2-6 linkages, whereasB. ovatusferments inulin with β2-1 linkages. Since inulin and levan are both fructose polymers, inulin and levan diet have similar perceptual salience to mice. We find that mice colonized withB. thetaiotaomicronprefer the non-fermentable inulin diet, while mice colonized withB. ovatusprefer the non-fermentable levan diet. Knockout of bacterial fructan utilization genes abrogates this preference, whereas swapping the fermentation ability ofB. thetaiotaomicronto inulin confers host preference for the levan diet. Bacterial fructan fermentation and host behavioral preference for the non-fermentable fructan are associated with increased neuronal activation in the arcuate nucleus of the hypothalamus, a key brain region for appetite regulation. These results reveal that selective nutrient metabolism by gut bacteria contributes to host associative learning of dietary preference, and further informs fundamental understanding of the biological determinants of food choice.
Greater leaf photosynthesis in the field by increasing mesophyll conductance via modified cell wall porosity and thickness in tobacco
Authors: Salesse-Smith Coralie E. , Lochocki Edward B. , Doran Lynn , Haas Benjamin E. , Stutz Samantha S. , Long Stephen P. 
Published: February 2024

AbstractMesophyll conductance (gm) describes the ease with which CO2passes from the sub-stomatal cavities of the leaf to the primary carboxylase of photosynthesis, Rubisco. Increasinggmhas been suggested as a means to engineer increases in photosynthesis by increasing [CO2] at Rubisco, inhibiting oxygenation and accelerating carboxylation. Here tobacco was transgenically up-regulated with Arabidopsis Cotton Golgi-related 3 (CGR3), a gene controlling methylesterification of pectin, as a strategy to increase CO2diffusion across the cell wall and thereby increasegm. Across three independent events in tobacco strongly expressingAtCGR3,mesophyll cell wall thickness was decreased by 7-13%, wall porosity increased by 75%, andgmmeasured by carbon isotope discrimination increased by 28%. Importantly, field-grown plants showed an average 8% increase in leaf photosynthetic CO2uptake. UpregulatingCGR3provides a new strategy for increasinggmin dicotyledonous crops, leading to higher CO2assimilation and a potential means to sustainable crop yield improvement.
Associations between multiple immune-response-related proteins and neonatal infection: a proximity extension assay based proteomic study in cord plasma of twins
Authors: Chen Ruoqing , Tan Weiri , Zheng Yeqi , Wu Feng , Liang Hui , Chen Youmei , Liu Xian , Fang Fang , Zhang Rui , Zhang Quanfu , Chen Xu 
Published: February 2024

AbstractBackgroundGiven their immature immune system, neonates are highly susceptible to infection, a major cause of neonatal death. However, associations between immune-response-related proteins and risk of neonatal infection have yet been systematically investigated.MethodsWe conducted a nested case-control study of 149 twins (60 cases and 89 controls, including 34 pairs of discordant twins), within the Shenzhen Baoan Birth and Twin (SZBBTwin) cohort. Using proximity extension assay of Olink Proteomics, 92 immune-response-related proteins were measured in samples of cord plasma. All twins were followed for a diagnosis of infection from birth until 27 days of age. Wilcoxon rank-sum test was used to determine differentially expressed proteins (DEPs), and multivariable logistic regression was used to assess the associations of the levels of proteins with neonatal infection. The receiver operating characteristic curve was plotted to evaluate the predictive performance of DEPs. Enrichment analysis was performed to annotate potential functions and pathways of DEPs.ResultsFive DEPs (ITGA11, FCRL6, DDX58, SH2D1A, and EDAR) were identified for neonatal infection. A higher cord plasma level of integrin alpha 11 (ITGA11) was associated with a higher risk of neonatal infection in both the analyses of all twins and discordant twins. The area under the curve achieved 0.835 for the five DEPs. The identified DEPs were mainly involved in immune function and protein binding, and most of them were enriched in the nuclear factor kappa-B pathway.ConclusionMultiple immune-response-related proteins in cord plasma, particularly ITGA11, are associated with the risk of neonatal infection.Key pointIn this nested case-control study, 92 immune-response-related proteins were measured in cord plasma by proximity extension assay. A higher level of ITGA11 was associated with a higher risk of neonatal infection, in the analyses of all twins and discordant twins.
Retrospective cohort analysis of surgical management for congenital diaphragmatic eventration in children
Authors: Alzahrani Khalid , Heng Lymeymey , Khen-Dunlop Naziha , Panait Nicoleta , Hervieux Erik , Grynberg Lucie , Abbo Olivier , Hameury Frédéric , Lavrand Frédéric , Maillet Olivier , Haffreingue Aurore , Lehn Anne , Napoli Cocci Stephan De , Habonimana Edouard , Michel Jean-Luc , Montalva Louise , Ballouhey Quentin , Fotso Kamdem Arnaud , Lecompte Jean-François , Liné Antoine , Poupalou Anna , Meignan Pierre , Deslandes Loren , Podevin Guillaume , Schmitt Françoise 
Published: February 2024

AbstractAimTo compare the different surgical approaches for treating congenital diaphragmatic eventration (CDE) in children.MethodsRetrospective data analysis of a multicentric cohort of pediatric patients operated on for CDE between 2010 and 2021, with comparison of the different surgical approaches and their outcomes.Results112 patients, aged 12 [5–21] months, were operated on for CDE. Diaphragmatic plication was performed using thoracoscopy or RATS in 69 (62%) cases, postero-lateral thoracotomy (PLT) in 15 (13%), and using an abdominal approach in 28 (25%). Relief of symptoms was obtained in 88% of the cases and improvement in the diaphragmatic level in 90%. We recorded 31 postoperative complications (28%) and eight recurrences (7%). Compared to other approaches, PLT multiplied the duration of intravenous analgesia by three (96 vs 36h, p<0.0001) and the length of hospital stay by two (8 vs 4d, p=0.002). RATS provided more perioperative hepatic injuries and equivalent short-term results than thoracoscopy, but all five patients remained symptomatic and two of them experienced chest wall deformities.ConclusionDiaphragmatic plication via a minimally invasive thoracic approach may be the best treatment option for cases of symptomatic CDE. Further is required to confirm that RATS is, at least, not inferior to thoracoscopy.ClinicalTrialNCT04862494, 2021-04-28;
The decay of location advantages and the substitutive role of firm-specific advantages in technology-based manufacturing
Authors: Courtens Fiona Maureen , Haezendonck Elvira , Verbeke Alain 
Published: February 2024

Purpose
This research aims to provide a new perspective on the evolving linkages between LAs and FSAs in the context of the technology-based manufacturing industry. Firm-level competitive strengths in an international context build upon the combination of (largely) exogenous location advantages (LAs) and endogenous firm-specific advantages (FSAs). The authors focus especially on the decay of LAs over time, which has been observed in many highly developed countries during the past decades. The authors show how the strengthening of FSAs can substitute for decaying LAs, thereby safeguarding against the demise of entire industrial regions.

Design/methodology/approach
The authors examine the technology-based manufacturing industry in Belgium, building upon an analysis of survey responses by 66 firms including a subgroup of 26 multinational enterprise (MNE) subsidiaries. The professional association representing this industry in Belgium (Agoria) viewed the firms included in the survey as representative for Belgian technology-based manufacturing in terms of the LAs they presently build upon (or location disadvantages they face) and the internal strengths they command relative to (foreign) rivals. The investigation uncovered the decay of critical LAs in Belgium and in parallel, the rise of ‘compensating’ FSAs of Belgian operations relative to foreign firms, including, especially, MNE sister subsidiaries in other countries. The authors also conducted 23 in-depth interviews with senior level managers (CEOs and senior vice presidents) of technology-based firms, including 10 subsidiaries of foreign-owned MNEs, which validated our analysis of the interplay between LAs and FSAs.

Findings
The findings reveal that since inception, Belgian manufacturing operations experienced an overall decay in their critical LAs by 23% on average. Despite this, several Belgian subsidiaries of foreign MNEs consider themselves as commanding a resource-base superior to that of the next-best-in-class subsidiaries. Furthermore, when assessing the dynamic interplay between LAs and FSAs, there is some evidence that the decay of LAs fueled the quest for – and firm-level journey toward – stronger FSAs.

Originality/value
The originality of this study is the alternative perspective to the conventionally assumed “positive-positive” relationship between LAs and FSAs. Prior management research has not examined the impact of decaying LAs on new FSA-creation in the realm of technology-based manufacturing.
The three levels of organizational change to build future potential organizations
Authors: Krajcsák Zoltán , Bakacsi Gyula 
Published: February 2024

Purpose
This study aims to answer the question of what characterizes organizations with future-potential, and with the help of a model introduced in this study, the authors propose what interventions can be identified and which improvements need to be made in traditional organizations so that they meet the requirements of future-potentiality.

Design/methodology/approach
A systematic literature review was conducted to identify management interventions. Citation and co-word analyses were also performed. Content analysis of 311 journal articles from the past five years was performed taking into account relevant keywords, and disciplinary narrowing was also applied. These articles were used to identify knowledge that could be used to suggest micro-, meso- and macro-level changes.

Findings
To develop the future potential of organizations, three organizational levels must be separated. The first is the micro level of relations between leaders and employees, where equity is a key value for future potentiality. It should be emphasized that not all employees’ organizational commitment is equally important for organizations with future potential, and leaders should strengthen their commitment according to individual needs and opportunities. The second is the meso level, where the decisive value is organizational moderation, and this suggests that a careful and restrained development is needed both in satisfying consumer needs and in innovation. The third is the macro level, where the defining value is responsibility and sustainability, which are necessary for achieving a state where the active development of national culture becomes possible.

Originality/value
Contrary to the authors’ expectations, it has been found that there are only a few studies dealing with change management for the purpose of achieving a future potential mode of organizational operation; thus, the results can be considered new and will contribute to the development of a cross-section of change management and future studies.