bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatio-temporal organization of network activity patterns in the hippocampus
Authors: Lopes-dos-Santos Vítor , Brizee Demi , Dupret David 
Published: October 2023

AbstractThe hippocampus is a layered brain network, composed of diverse cell types arranged in multiple microcircuits with anatomically structured inputs, all working in concert to support memory. This intricate organization generates a myriad of electrophysiological signatures. While specific aspects of these activity patterns have been explored, a comprehensive understanding of the hippocampal layer-embedded dynamics remains elusive. Here, we developed a low-dimensional manifold to capture electrophysiological patterns, mapping their anatomical trajectory along the CA1-to-DG axis, and distinguishing layers based on sharp-wave and theta profiles. This profiling led to the characterization of selective theta-nested gamma signatures for each layer. It further revealed spike patterns associated with gamma rhythms, which highlight specific firing motifs between principal cells and interneurons, and differential firing properties within pyramidal sub-layers. These findings support a holistic understanding of the spatio-temporal activity patterns across hippocampal layers for unraveling the network operations that drive memory-guided behavior.
Chemical-LTP induces confinment of BDNF mRNA under dendritic spines and BDNF protein accumulation inside the spines
Authors: Bimbi Giorgia , Tongiorgi Enrico 
Published: October 2023

AbstractThe neurotrophin brain-derived neurotrophic factor (BDNF) plays a key role in neuronal development and synaptic plasticity. The discovery that BDNF mRNA can be transported in neuronal dendrites in an activity-dependent manner has suggested that its local translation may support synapse maturation and plasticity. However, a clear demonstration that BDNF mRNA is locally transported and translated at activated synapses in response to long-term potentiation (LTP) is still lacking. Here, we study the dynamics of BDNF mRNA dendritic trafficking following induction of chemical-LTP (cLTP). Dendritic transport of BDNF transcripts was analysed using the MS2 system for mRNA visualization, and chimeric BDNF-GFP constructs were used to monitor protein synthesis in living neurons. We found that within 15’ following cLTP induction, most BDNF mRNA granules become stationary and transiently accumulate in the dendritic shaft at the basis of the spines similarly to the control CamkIIα mRNA which increased also inside the spines, at 60’ post-cLTP. At 60’ but not at 15’ from cLTP induction, we observed an increase in BDNF protein levels within the spine. Taken together, these findings suggest that BDNF mRNA trafficking is arrested in the early phase of cLTP, providing a local source of mRNA for translation of BDNF at the basis of the spine followed in the late LTP phase, by translocation of the BDNF protein within the spine head.StatementBrain-derived neurotrophic factor (BDNF) plays a key role in neuronal development and synaptic plasticity. In this study, we investigate two unresolved questions in neuronal plasticity: a) whether the post-synaptically released BDNF can be locally synthesized in this compartment, and b) whether the local translation of BDNF occurs in dendrites, or within the spine. Using chimeric constructs ectopically expressed in living primary hippocampal neurons, we tracked BDNF mRNA trafficking within the dendrites and its local translation following induction of chemical-LTP (cLTP) by forskolin. We show that in the early phase of cLTP induction (15’), BDNF mRNA becomes confined at the basis of the spines providing a local source for translation of the protein followed in the late LTP phase (60’), by translocation of the BDNF protein within the spine head.
Microglia are dispensable for experience-dependent refinement of visual circuitry
Authors: Brown Thomas C. , Crouse Emily C. , Attaway Cecilia A. , Oakes Dana K. , Minton Sarah W. , Borghuis Bart G. , McGee Aaron W. 
Published: October 2023

AbstractMicroglia are proposed to be critical for the refinement of developing neural circuitry. However, evidence identifying specific roles for microglia has been limited and often indirect. Here we examined whether microglia are required for the experience-dependent refinement of visual circuitry and visual function during development. We ablated microglia by administering the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622, and then examined the consequences for retinal function, receptive field tuning of neurons in primary visual cortex (V1), visual acuity, and experience-dependent plasticity in visual circuitry. Eradicating microglia by treating mice with PLX5622 beginning at postnatal day (P) 14 did not alter visual response properties of retinal ganglion cells examined three or more weeks later. Mice treated with PLX5622 from P14 lacked more than 95% of microglia in V1 by P18, prior to the opening of the critical period. Despite the absence of microglia, the receptive field tuning properties of neurons in V1 were normal at P32. Similarly, eradicating microglia did not affect the maturation of visual acuity. Mice treated with PLX5622 displayed typical ocular dominance plasticity in response to brief monocular deprivation. Thus, none of these principal measurements of visual circuit development and function detectibly differed in the absence of microglia. We conclude that microglia are dispensable for experience-dependent refinement of visual circuitry. These findings challenge the proposed critical role of microglia in refining neural circuitry.
Phages Produce Persisters
Authors: Fernández-García Laura , Kirigo Joy , Huelgas-Méndez Daniel , Tomás María , García-Contreras Rodolfo , Wood Thomas K. 
Published: October 2023

SUMMARYBacteria primarily encounter stress, and, arguably, their greatest threats are phages. It is often assumed that those bacteria that escape phage attack have mutated; however, another possibility is that a subpopulation forms the dormant persister state, in a manner similar to that demonstrated for bacterial cells undergoing nutritive, oxidative, and antibiotic stress. Persister cells do not undergo mutation and survive lethal conditions by ceasing growth transiently. Slower growth and dormancy play a key physiological role as they allow host phage defense systems more time to clear the phage infection. Here we investigated how bacteria survive lytic phage infection by isolating surviving cells from the plaques of T2, T4, and lambda (cI mutant) virulent phages. We found that bacteria in plaques can escape phage attack both by mutation (i.e., become resistant) and without mutation (i.e., become persistent). Specifically, whereas T4-resistant and lambda-resistant bacteria with over a 100,000-fold less sensitivity were isolated from plaques with obvious genetic mutations (e.g., causing mucoidy), cells were also found after T2 infection that undergo no significant mutation, retain wild-type phage sensitivity, and survive lethal doses of antibiotics. Corroborating this, adding T2 phage to persister cells resulted in 137,000-fold more survival compared to that of addition to exponentially-growing cells. Phage treatments withKlebsiella pneumoniaandPseudomonas aeruginosaalso generated persister cells. Hence, along with resistant strains, bacteria also form persister cells during phage infection.
A Histomorphometric and Computational Investigation of the Stabilizing Role of Pectinate Ligaments in the Aqueous Outflow Pathway
Authors: Safa Babak N. , Fraticelli Guzmán Nina Sara , Li Guorong , Stamer W. Daniel , Feola Andrew J. , Ethier C. Ross 
Published: October 2023

AbstractMurine models are commonly used to study glaucoma, the leading cause of irreversible blindness. Glaucoma is associated with elevated intraocular pressure (IOP), which is regulated by the tissues of the aqueous outflow pathway. In particular, pectinate ligaments (PLs) connect the iris and trabecular meshwork (TM) at the anterior chamber angle, with an unknown role in maintenance of the biomechanical stability of the aqueous outflow pathway, thus motivating this study. We conducted histomorphometric analysis and optical coherence tomography-based finite element (FE) modeling on three cohorts of C57BL/6 mice: ‘young’ (2-6 months), ‘middle-aged’ (11-16 months), and ‘elderly’ (25-32 months). We evaluated the age-specific morphology of the outflow pathway tissues. Further, because of the known pressure-dependent Schlemm’s canal (SC) narrowing, we assessed the dependence of the SC lumen area to varying IOPs in age-specific FE models over a physiological range of TM/PL stiffness values. We found age-dependent changes in morphology of outflow tissues; notably, the PLs were more developed in older mice compared to younger ones. In addition, FE modeling demonstrated that murine SC patency is highly dependent on the presence of PLs, and that increased IOP caused SC collapse only with sufficiently low TM/PL stiffness values. Moreover, the elderly model showed more susceptibility to SC collapse compared to the younger models. In conclusion, our study elucidated the previously unexplored role of PLs in the aqueous outflow pathway, indicating their function in supporting TM and SC under elevated IOP.
Mechanism of 2’-Fucosyllactose degradation by Human-AssociatedAkkermansia
Authors: Padilla Loren , Fricker Ashwana D. , Luna Estefani , Choudhury Biswa , Hughes Elizabeth R. , Panzetta Maria E. , Valdivia Raphael H. , Flores Gilberto E. 
Published: October 2023

ABSTRACTAmong the first microorganisms to colonize the human gut of breastfed infants are bacteria capable of fermenting human milk oligosaccharides (HMOs). One of the most abundant HMOs, 2’-fucosyllactose (2’-FL), may specifically drive bacterial colonization of the intestine. Recently, differential growth has been observed across multiple species ofAkkermansiaon various HMOs including 2’FL. In culture, we found growth of two species,A. muciniphilaMucTandA. biwaensisCSUN-19, in HMOS corresponded to a decrease in the levels of 2’-FL and an increase in lactose, indicating that the first step in 2’-FL catabolism is the cleavage of fucose. Using phylogenetic analysis and transcriptional profiling, we found that the number and expression of fucosidase genes from two glycoside hydrolase (GH) families, GH29 and GH95, varies between these two species. During mid-log phase growth, the expression of several GH29 genes was increased by 2’-FL in both species, whereas the GH95 genes were induced only inA. muciniphila. We further show that one putative fucosidase and a β-galactosidase fromA. biwaensisare involved in the breakdown of 2’-FL. Our findings indicate that that plasticity of GHs of human associatedAkkermansiasp. enable access to additional growth substrates present in HMOs, including 2’-FL. Our work highlights the potential forAkkermansiato influence the development of the gut microbiota early in life and expands the known metabolic capabilities of this important human symbiont.IMPORTANCEAkkermansiaare mucin degrading specialists widely distributed in the human population.Akkermansia biwaensishas recently been observed to have enhanced growth relative to other human associatedAkkermansiaon multiple human milk oligosaccharides (HMOs). However, the mechanisms for enhanced growth are not understood. Here, we characterized the phylogenetic diversity and function of select genes involved in growth ofA. biwaensison 2’-fucosyllactose (2’-FL), a dominant HMO. Specifically, we demonstrate that two genes in a genomic locus, a putative β-galactosidase and α-fucosidase, are likely responsible for the enhanced growth on 2’-FL. The functional characterization ofA. biwaensisgrowth on 2’-FL delineates the significance of a single genomic locus that may facilitate enhanced colonization and functional activity of selectAkkermansiaearly in life.
The influence of skin pigmentation on pulse oximetry readings: a protocol for systematic review and meta-analysis
Authors: Ahmed Shabbir , Afrin Sadia , Hasan SK Mehedi , Sarker Sharmishtha Sonalika , Azrin Farhia , Jahan Esrat , Rahman Ahmed Ehsanur 
Published: October 2023

AbstractBackgroundPulse oximeters are widely used crucial devices to triage and monitor sign of diseases and make informed decisions on patients’ health. Various studies have found a bias in pulse oximetry readings based on skin pigmentation/ ethnicity. This protocol outlines a systematic review and meta-analysis approach that will assess the effect of different skin pigmentation levels on the accuracy of pulse oximetry readings.Methods and analysisWe will conduct search in Medline, Embase, CINAHL, and Web of Science database. Studies carried out either on healthy population or population with critical illness measuring pulse oximetry (SpO2) versus SaO2 obtained by standard CO-oximetry based on skin pigmentation and/or ethnicity and reported in the form of overall accuracy, bias, precision, or agreement will be included in this study. Four dedicated reviewers will independently assess and extract data from the included studies in a pre-specified excel format for further analysis. Any discordance in the way will be resolved by discussion until consensus is reached. JBI’s critical appraisal tool for diagnostic test accuracy test will be used to assess study quality for the included studies.Ethics and disseminationEthical approval is not required because no original data will be collected as part of this review. The final review will be submitted to a peer-reviewed journal for publication and presented at conferences.
How cultural values integrate with each other to trigger sustainable consumption: a cross-culture study
Authors: Duong Cong Doanh , Le Thi Loan , Lee Eun-Mi , Gadomska-Lila Katarzyna 
Published: October 2023

PurposeThis cross-culture study aims to investigate how two cultural values, collectivism (COL) and long-term orientation (LTO), integrate with each other to trigger green consumption.Design/methodology/approachUsing data from three consumer surveys in Vietnam, South Korea and Poland, this study employs polynomial regression with response surface analysis as a methodological approach to assess the complementary, balanced and imbalanced effects of cultural dimensions (COL and LTO) on consumers' green purchase intention (GPI) and behaviors.FindingsFirst, this study found that, in Vietnam, both COL and LTO had a significant positive effect on GPI. However, only COL demonstrated a significant effect on GPI in South Korea and Poland. Second, this study also revealed that, in all three countries, when COL and LTO were in agreement, the degree of GPI was higher when COL and LTO were higher. The findings also showed that an increase in the imbalance between COL and LTO integration in the Vietnam sample led to a decrease in consumers' GPI. However, this relationship was insignificant in the South Korea and Poland samples.Originality/valueThis study enriches the understanding of green purchase behavior (GPB) and its underlying cultural factors within a cross-cultural framework. In particular, it enhances the knowledge of the debated relationship between different facets of cultural values (specifically, COL and LTO) and pro-environmental behavior, shedding light on this complex relationship in the context of the three different countries.
Inhibitory influence of supply chain digital transformation on bullwhip effect feedback difference
Authors: Gao Jing , Gao Yang , Guan Tao , Liu Sisi , Ma Tao 
Published: October 2023

PurposeThis paper breaks through the limitations of the research on bullwhip effect in the traditional supply chain, extends the research perspective to digital supply chain and discusses the weakening effect of digital supply chain on bullwhip effect by comparing the overall performance of the two.Design/methodology/approachThis paper starts with the weakening mechanism of supply chain digitization on bullwhip effect, builds bullwhip effect models of traditional supply chain and digital supply chain, respectively, simulates the influence of supply chain digitization transformation on bullwhip effect by using Matlab software and analyzes the causes of bullwhip effect in supply chain led by T company and the digitization process.FindingsFirstly, digitization can reduce bullwhip effect in multi-level supply chain by reducing information feedback deviation. Second, digital transformation is conducive to improving the overall performance of the supply chain. Third, government incentives can promote the digital transformation of supply chain and inhibit bullwhip effect.Research limitations/implicationsAlthough the study considers the heterogeneous subject -- the government's incentive effect on digital transformation and information sharing – it does not include the influence of the end node in the supply chain, that is the consumer. In addition, this paper only analyzes and discusses the bullwhip effect on the amplification of demand, without considering the situation that the market contraction will lead to the reduction of demand.Practical implicationsThis paper considers the distortion degree and delay degree of information feedback, carries out quantitative analysis of bullwhip effect, builds the bullwhip effect model of traditional supply chain and digital supply chain, uses Matlab software to analyze the difference of the influence of supply chain digital transformation on bullwhip effect suppression and puts forward the corresponding control strategy.Social implicationsThe research shows that digital transformation can reduce the bullwhip effect in multi-layer supply chain by reducing the information feedback deviation, which is conducive to improving the overall supply chain performance, and government support can accelerate the digital transformation of supply chain to a certain extent.Originality/valueFirst, break through the limitations of traditional supply chain research, expand the research perspective to digital supply chain and discuss the weakening effect of digital supply chain on bullwhip effect by comparing the overall performance of the two. Second, quantify the bullwhip effect through information feedback bias and provide an analysis method for the weakening of the bullwhip effect. Third, the driving role of the government in the digital transformation of the supply chain is considered in the study, so that the model is more close to the actual situation of enterprise operation.
Meshfree algorithms for analysis and computational modeling of multidimensional hyperbolic wave models
Authors: Pandit Sapna , Verma Pooja , Kumar Manoj , Poonam  
Published: October 2023

PurposeThis article offered two meshfree algorithms, namely the local radial basis functions-finite difference (LRBF-FD) approximation and local radial basis functions-differential quadrature method (LRBF-DQM) to simulate the multidimensional hyperbolic wave models and work is an extension of Jiwari (2015).Design/methodology/approachIn the evolvement of the first algorithm, the time derivative is discretized by the forward FD scheme and the Crank-Nicolson scheme is used for the rest of the terms. After that, the LRBF-FD approximation is used for spatial discretization and quasi-linearization process for linearization of the problem. Finally, the obtained linear system is solved by the LU decomposition method. In the development of the second algorithm, semi-discretization in space is done via LRBF-DQM and then an explicit RK4 is used for fully discretization in time.FindingsFor simulation purposes, some 1D and 2D wave models are pondered to instigate the chastity and competence of the developed algorithms.Originality/valueThe developed algorithms are novel for the multidimensional hyperbolic wave models. Also, the stability analysis of the second algorithm is a new work for these types of model.
Redefining banks in the digital era: a typology of banks and their research, managerial and policy implications
Authors: Mogaji Emmanuel 
Published: October 2023

PurposeThis study aims to shed light on the evolving nature of banks in the digital era and the implications for bank marketing and management. The research addresses the need for a comprehensive typology of banks that integrates fintech and explores how traditional and app-only banks strategically position their brands. The key argument is that understanding the changing landscape of banking and the impact of technological advancements is crucial for banks to navigate the challenges and opportunities presented by fintech and digital transformation.Design/methodology/approachThis study examines literature and practices to develop a typology of banks, describing their characteristics, strengths, weaknesses and providing examples. It also proposes new research agendas for scholars and practitioners in the field.FindingsThis paper introduces a typology of banks based on their adoption of fintech and digital technologies. Three distinct types of banks are identified: Traditional banks adopting FinTech (TBAF), Traditionally Driven Neo Banks (TDNBs) and Digitally Driven Neo Banks (DDNBs). TBAF are traditional banks that have embraced fintech solutions to enhance their operations and customer experiences. TDNBs represent a hybrid model, combining the trusted brand and infrastructure of traditional banks with the digital capabilities and agility of neo banks. DDNBs are purely digital banks that operate exclusively online, offering innovative and user-friendly banking services.Originality/valueThis study is a pioneering work that classified banks based on their utilization of fintech and digital technologies. The study provides a typology of banks based on fintech adoption, offering valuable insights for bank managers, policymakers and researchers. The research also outlines a research agenda, suggesting future investigations to further enhance understanding of the evolving banking landscape and its implications.
Sale to list ratio, for-sale inventory, sale count, and housing value
Authors: Zhang Xingrui , Wang Yunpeng , Yang Eunhwa , Xu Shuai , Yu Yihang 
Published: October 2023

Purpose
The purpose of the paper is twofold: first, to observe the relationship between sale to list ratio (SLR)/ for-sale inventory (FSI)/ sale count nowcast (SCN) and real/nominal housing value, and second, to produce a handbook of empirical evidence that can serve as a foundation for future research on this topic.

Design/methodology/approach
This paper broadly compiles empirical evidence, using three of the most common causality tests in the field of housing economics. The analysis methods include lagged Pearson correlation test, Granger causality test and cointegration test.

Findings
Causal relationships were observed between SLR/FSI/SCN and both nominal and real housing values. SLR and SCN showed positive long-term correlations with housing value, whereas FSI had a negative correlation. Adjusting the housing value with the Consumer Price Index (CPI) to derive real housing values could potentially alter the direction of the causal relationships. It is crucial to distinguish the long-term relationship from temporal dynamics, as FSI displayed a positive immediate impulse–response relationship with nominal housing price despite the negative long-term correlation.

Originality/value
SLR/FSI/SCN are housing market parameters that have only recently begun to be documented and have seen little use in research. So far, housing market research has revolved around traditional macroeconomic indicators such as unemployment rate. To the best of the authors’ knowledge, this study is one of the first studies that introduce these three parameters into housing market research.