bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case series of the twiddler syndrome
Authors: Montisci Roberta , Soro Cinzia , Demelas Roberta , Agus Elena , Follesa Alessio , Siragusa Gesualdo , Nissardi Vincenzo 
Published: January 2024

Abstract

Background
Twiddler syndrome (TS) is a complication of cardiac implantable electronic device (CIED) implantation, caused by the deliberate or unconscious manipulation of the device by the patient himself, which results in dislocation of the leads by retraction towards the subcutaneous pocket.

Case summary
This report describes two clinical cases that occurred in our centre, for which two different solutions were successfully implemented. In the first case, a complete removal of the stimulation system was performed, and a leadless pacemaker (PM; Medtronic Micra VR) was implanted. In the second case, the patient underwent a revision procedure. The PM was disconnected, and the electrodes were debrided, a submuscular pocket for the PM was created, and at the end of the procedure, the PM was anchored to the pectoralis major.

Discussion
Twiddler syndrome is a not so rare and serious complication of CIED implantation, leading to device malfunctioning and higher risk of infection of the pocket due to multiple re-interventions. In these two cases, different surgical solutions were performed, both resulting to be effective to solve the effects of TS.
Electrophysiological characteristics and ablation of ventricular arrhythmias originating from the intramural basal inferior septum
Authors: Yang Jie , Li Mengmeng , Jiang Chenxi , Tang Ribo , Sang Caihua , Wang Wei , Zhao Xin , Li Changyi , Li Songnan , Guo Xueyuan , Jia Changqi , Ning Man , Feng Li , Wen Dan , Zhu Hui , Jiang Yuexin , Liu Tong , Liu Fang , Long Deyong , Dong Jianzeng , Ma Changsheng 
Published: January 2024

Abstract

Aims
The electrocardiographic and electrophysiological characteristics of ventricular arrhythmia (VA) arising from the intramural basal inferior septum (BIS) have not been specifically addressed to date. The aim of the current study was to characterize intramural BIS-VA and distinguish it from those with endocardial origins besides clarifying the anatomical configurations of the pyramidal space.

Methods and results
Fifty-five consecutive patients undergoing catheter ablation of VAs from BIS were identified and divided into three groups: the left ventricular (LV)-BIS group (n = 28), right ventricular (RV)-BIS group (n = 8), and intramural group (Intra, n = 19). Compared with the LV-BIS and RV-BIS groups, patients in the Intra group presented with no adequate earliest activation time at the two-sided BIS and epicardial coronary system [right: 7.79 ± 2.38 vs. left: 7.16 ± 2.59 vs. the middle cardiac vein (MCV): 6.26 ± 1.73 ms, P = 0.173] and poor-matched pacing-produced QRS at each site. Under the intracardiac echocardiography view, the pyramidal base was the broadest part of the septum and served as the division of the two-sided BIS. Focal ablation yielded promising acute-term and long-term procedural success in the LV-BIS and RV-BIS groups. But for the Intra group, VAs disappeared only after stepwise ablation successively targeted early preferential exit. After follow-up, three patients in the Intra group had recurrent VA, and all of them were treated well by a redo procedure or drug therapy.

Conclusion
Intramural VAs were relatively common in the BIS region in our series. Intra-procedural mapping was important to distinguish the intramural VAs from other VAs by comparing the local activation time and pacing mapping. Procedural success could be achieved by stepwise ablation on the counterpart sides of the BIS and within the MCV.
Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform
Authors: Kampmann Marie-Louise , Fleckhaus Jan , Børsting Claus , Jurtikova Helena , Piters Alice , Papin Julien , Gauthier Quentin , Ghemrawi Mirna , Doutremepuich Christian , McCord Bruce , Schneider Peter M , Drabek Jiri , Morling Niels 
Published: January 2024

Abstract

Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set.

Key points
Degenerate orthorhombic models
Authors: Stovas Alexey , Roganov Yuriy , Roganov Vyacheslav 
Published: January 2024

SUMMARY
We define the degenerate orthorhombic anisotropy models which have two symmetric singularity lines with constant phase velocity for S1 and S2 waves. Depending on the singularity line trajectory, we consider two types of degenerate models (VTI- and HTI-type). In addition to this singularity line, seriethere is always one isolated singularity point in one of non-essential symmetry planes. The degenerate orthorhombic model has seven independent parameters and can be parametrized by different combinations of the stiffness coefficients. Exploiting the fact that the second-order derivatives matrix computed from the Christoffel polynomial is degenerate, we also compute the group velocity image of this singularity line.
‘Double Puzzle’ at the Shumagin seismic gap, Alaska Peninsula: intraslab strike-slip faulting loaded by lateral variations in megathrust fault friction
Authors: Senapati Batakrushna , Lindsey Eric O , Kundu Bhaskar , Panda Dibyashakti , Tiwari Deepak K , Yadav Rajeev Kumar 
Published: January 2024

SUMMARY
Along the Aleutian megathrust, the Shumagin seismic gap (162° W–158.5° W) had not hosted a large megathrust earthquake during the observational period 1946–2020. Geodetic evidence suggests a prominent trench-parallel transition from strong to weak kinematic coupling in this segment, indicating varying frictional properties of the megathrust. In 2020 July and October, the occurrence of two large, but dissimilar earthquakes in this seismic gap (the Mw 7.8 Simeonof Island thrust event on 2020 July 22 followed by the Mw 7.6 Sand Point intraslab strike-slip event on 2020 October 19) presented a unique opportunity to examine the interaction between stresses on the megathrust and within the downgoing slab. We use geodetic and geophysical evidence to derive a more accurate kinematic coupling model of the megathrust in this area and show that the Mw 7.6 Sand Point earthquake within the downgoing Pacific slab, likely occurred as a result of both trench-perpendicular interseismic shear stress caused by variable kinematic coupling of the megathrust and coseismic stress changes resulting from the Simeonof Island event. Furthermore, we show that the location of the strike-slip event coincides with along-strike change in the megathrust gravitational anomaly and flexural bending of the downgoing plate, suggesting a long-term interaction between megathrust frictional properties and the structure of the plate interface and downgoing slab.
National action plans on antimicrobial resistance in Latin America: an analysis via a governance framework
Authors: Avello Paula , Collins Lisa M , Gómez Sonia A , Luna Federico , Fernández Miyakawa Mariano E , West Helen M , Iossa Graziella 
Published: January 2024

Abstract
In 2015, the World Health Assembly adopted a global action plan (GAP) on antimicrobial resistance (AMR). Member states were encouraged to develop their own national action plans (NAPs) in alignment with the GAP. To-date, in systematic assessments of NAPs, the Latin American specific context has not been previously analysed. Here we examined 11 Latin American NAPs published between 2015 and 2021 using content analysis. We focused on two approaches: (1) alignment between the strategic objectives and actions defined in the GAP, and those outlined in the NAPs via a content indicator; and (2) assessment of the NAPs via a governance framework covering ‘policy design’, ‘implementation tools’ and ‘monitoring and evaluation’ areas. We observed a high alignment with the strategic objectives of the GAP; however, the opposite was observed for the corresponding actions. Our results showed that the governance aspects contained within coordination and participation domains were addressed by every Latin American NAP, whereas monitoring and assessment areas, as well as incorporating the environment, would need more attention in subsequent NAPs. Given that AMR is a global health threat and collective efforts across regions are necessary to combat it, our findings can benefit member states by highlighting how to strengthen the AMR strategies in Latin America, while also supporting global policy formulation.
Management strategy evaluation of harvest control rules for Pacific Herring in Prince William Sound, Alaska
Authors: Zahner Joshua A , Branch Trevor A 
Published: January 2024

Abstract
Management strategy evaluation (MSE) provides a mechanism to test the relative performance of alternative management strategies on a fishery. For Pacific herring in Prince William Sound, Alaska, no directed fisheries have occurred in over 30 years, providing an opportunity to evaluate potential management strategies before a fishery is opened. Here we evaluate and compare 10 harvest control rules (HCRs) ranging from simple threshold rules to rules accounting for population age structure, biomass trends, and weight distribution using an MSE integrated with a fully Bayesian stock assessment estimation model. We developed a utility function that shows simple threshold HCRs outperform the more complex rules, especially for catch stability. According to this utility function, the best rule had a lower limit threshold than the current default rule, while the worst rule had a higher limit threshold. Our simulations demonstrate that sufficient computing power exists for MSEs based on Bayesian estimation methods, thus opening a pathway for MSEs to simulation test probabilistic control rules, which provide a buffer against scientific uncertainty and should reduce the risk of overfishing.
El proceso constituyente de Bachelet en Chile (2015-2018): razones de un fracaso (previsible)Bachelet’s constitution-making process in Chile (2015-2018): reasons for a (foreseeable) failure
Authors: Henríquez Viñas Miriam , Francisco García José 
Published: January 2024

Abstract
This essay contributes to the increasing comparative constitutional law literature that has been examining constitution-making failure, the factors that explains it, and the possibility of obtaining valuable lessons for the design of future processes. For this purpose, it examines the Chilean constitution-making process of 2015-2018, led by former President Michelle Bachelet. The article shows that the most relevant factors that explains this failure relates to a dynamic political and social context, the institutional design of the process, and the exclusion of political parties of the process and the lack of consensus among the political elite. However, this failure cannot be considered definitive and total. To the contrary, it was only temporary and partial, considering the influence that the process had over the next two constitution-making process that followed on the next years (2019-2022, and 2023).
Career outlook and satisfaction in the presence of workload intensification—a survey of early career pharmacists
Authors: Bradley Fiona , Hammond Michael , Braund Rhiannon 
Published: January 2024

Abstract

Background
The demographics of the pharmacy workforce is changing with an increased proportion of pharmacists less than 30 years old (early career pharmacists—ECPs). In parallel, the profession has experienced workload intensification and workforce attrition. It is important to understand ECPs career satisfaction to retain this section of the pharmacy profession.

Objectives
This study aimed to collect data on the current career satisfaction of ECPs, and identify workplace factors that were most important to this group. Further, to use these findings to inform sector recommendations.

Methods
A steering group of ECPs in Aotearoa New Zealand developed a survey based on one used previously. An invitation email was sent to all pharmacists who had been registered for less than 10 years and were members of the Pharmaceutical Society of New Zealand.

Results
A total of 1418 ECPs were identified and invited to participate, and responses were received by 416 of these. While 90% believe that they are making a useful contribution to the health of their patients, over half are unhappy and discontented, with over a third dissatisfied with their careers. A large proportion (44%) were considering leaving the profession in the next 5 years. The top three factors for career satisfaction were ability to progress and learn new skills, the people they work with, and the remuneration.

Conclusions
This study provides a starting point for understanding the current environment and level of dissatisfaction of young pharmacists. There are several areas of concern that need to be addressed if a strong vibrant viable pharmacy profession is to be achieved.
Persistent Low-Level Variants in a Subset of Viral Genes Are Highly Predictive of Poor Outcome in Immunocompromised Patients With Cytomegalovirus Infection
Authors: Venturini Cristina , Colston Julia M , Charles Oscar , Lankina Anastasia , Best Timothy , Atkinson Claire , Forrest Calum , Williams Charlotte A , Rao Kanchan , Worth Austen , Thorburn Doug , Harber Mark , Griffiths Paul , Breuer Judith 
Published: January 2024

Abstract

Background
Human cytomegalovirus (HCMV) is the most common and serious opportunistic infection after solid organ and hematopoietic stem cell transplantation. In this study, we used whole-genome HCMV data to investigate viral factors associated with the clinical outcome.

Methods
We sequenced HCMV samples from 16 immunocompromised pediatric patients with persistent viremia. Eight of the 16 patients died of complications due to HCMV infection. We also sequenced samples from 35 infected solid organ adult recipients, of whom 1 died with HCMV infection.

Results
We showed that samples from both groups have fixed variants at resistance sites and mixed infections. Next-generation sequencing also revealed nonfixed variants at resistance sites in most of the patients who died (6/9). A machine learning approach identified 10 genes with nonfixed variants in these patients. These genes formed a viral signature that discriminated patients with HCMV infection who died from those who survived with high accuracy (area under the curve = 0.96). Lymphocyte numbers for a subset of patients showed no recovery posttransplant in the patients who died.

Conclusions
We hypothesize that the viral signature identified in this study may be a useful biomarker for poor response to antiviral drug treatment and indirectly for poor T-cell function, potentially identifying early those patients requiring nonpharmacological interventions.
Insights into the antimicrobial effects of tafenoquine against Enterococcus and its biofilms
Authors: Wu Yuan , Li Huilong , Wu Pingyun , Wu Ruolan , She Pengfei 
Published: January 2024

Abstract

Aim
The purpose of this study is to assess the in vitro antimicrobial and anti-biofilm effects of the anti-protozoal agent tafenoquine (TAF) on Enterococcus and elucidate its underlying mode of action.

Methods and results
The present work investigated the susceptibility of TAF on 3 type strains and 11 clinical isolates of enterococci. The results indicated that TAF exhibited powerful antimicrobial activity against both of Enterococcus faecalis and Enterococcus faecium with minimum inhibitory and bactericidal concentrations ranging from 8 to 16 µg ml−1. Meanwhile, biofilm inhibition and eradication assays showed that TAF exhibited potent anti-biofilm activity against E. faecalis ATCC 29212 and E. faecium ATCC 19434. Ultra-microscopic observations revealed significant changes in bacterial morphology and structure caused by TAF, particularly for the disruption of plasma membrane. Mechanistic investigations also revealed that TAF altered both membrane permeability and potential while also impacting adenosine triphosphate production as well as reactive oxygen species generation. In addition, no detectable cytotoxicity of TAF on human cells was observed at concentrations near the minimal inhibitory concentration.

Conclusions
In summary, this study confirmed that TAF could effectively inhibit Enterococcus as well as its biofilm formation.
The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development
Authors: Song Xiaozhen , Hu Ruixing , Chen Yi , Xiao Man , Zhang Hong , Wu Shengnan , Lu Qing 
Published: January 2024

Abstract
TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways, contributing to immune responses, tumor progression, and embryonic development. Somatic mutations within the coiled-coil (CC) domain and WD40 repeat domain of TRAF7 could cause brain tumors, while germline pathogenic mutations contribute to severe developmental abnormalities. However, the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains unclear. In this study, we employed zebrafish as an in vivo model system. TRAF7 knock down caused defects in zebrafish embryonic development. We determined the crystal structure of TRAF7 CC domain at 3.3 Å resolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic development. Additionally, disease-causing mutations in TRAF7 CC region could impair the trimer formation, consequently impacting early embryonic development of zebrafish. Therefore, our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.
Perforated appendicitis due to fishbone
Authors: Hoang Van T , Hoang The H , Nguyen Hoang Q , Pham Ngoc T T , Vo Tien H , Chansomphou Vichit , Hoang Duc T 
Published: January 2024

Abstract
Appendicitis is a common condition in daily clinical practice. Appendicitis due to foreign bodies is uncommon and may result from obstruction or perforation mechanism. We present a rare case of a 43-year-old male patient who was diagnosed with perforated appendicitis due to a fish bone by imaging studies and confirmed postoperatively. Confirming the fish bone causing the perforation on images is sometimes difficult, requiring the radiologist to actively search and determine the source. In addition to appendectomy, the surgeon also needs to pay attention to removing all foreign objects and treating perforations of surrounding organs.
Inflammatory fibroid polyp of the small intestine presenting as small bowel obstruction with intussusception: a case report
Authors: Hehir Cian , Calpin Gavin , Dowling Gavin , Spillane Chloe , Kilgallen Clive , Hill Arnold D K 
Published: January 2024

Abstract
Inflammatory fibroid polyps (IFP) are rare benign neoplasms most commonly occurring within the respiratory tract but are rarely also observed in the gastro-intestinal tract. Herein we present the case of a 73-year-old female presenting with ileo-ileal intussusception secondary to IFP. The patient was treated with emergency laparotomy with segmental bowel resection and primary anastomosis. Histopathological analysis of the excised bowel segment initially revealed a low-grade, mural based spindle cell neoplasm with surrounding benign, reactive lymphadenopathy. Immunohistochemical analysis demonstrated that the lesional cells stained positive for Vimentin, Smooth Muscle Actin (SMA), and CD34. On secondary analysis of the specimen, the morphology and immunohistochemical profile of the mass was in keeping with IFP. No invasive malignancy was identified. Such cases have been previously reported under the pseudonym ‘the great mimicker’, due to their striking similarity to malignant processes. This case report aims to add to the small body of research reporting such atypical presentations.
A New Woman Dialogue with Aestheticism and Decadence: Netta Syrett’s Short Stories for The Yellow Book
Authors: Rose Lucy Ella 
Published: January 2024

ABSTRACT
Focusing on critically neglected works by prolific writer Netta Syrett (1865–1943), this article reveals her New Woman dialogue with aestheticism and decadence in her early short stories written for the iconic 1890s periodical The Yellow Book: primarily, ‘A Correspondence’ (1895) and ‘Far Above Rubies’ (1897). Together they trace Syrett’s increasingly assertive voice and navigation of the period’s seemingly competing but intersecting aesthetic, decadent and feminist movements. I argue that Syrett uses aesthetic and decadent discourses as strategic vehicles for the articulation of the evolving feminist ideas more fully expressed in her later pro-suffrage works. Specifically, her stories register her response to the male elitism and misogyny of aestheticism and decadence through a critical engagement with their tropes (exotic setting; aestheticized interior; femme fatale) and discourses (of mythology; statuary; floriography) in order to challenge the objectification and marginalization of women by masculinist culture using its own terms of reference. Syrett’s stories are thus discursive spaces through which she articulates anxieties about women’s place in, or exclusion from, aestheticism and decadence, asserting her role in these movements as both participant and critic. This article thus offers a more comprehensive understanding of the evolving discourses of, as well as the dialogues and debates enacted by, fin-de-siècle women’s writing, shedding new light on the aesthetic and decadent movements.
The Arabidopsis SR45 splicing factor bridges the splicing machinery and the exon–exon junction complex
Authors: Fanara Steven , Schloesser Marie , Joris Marine , De Franco Simona , Vandevenne Marylène , Kerff Frédéric , Hanikenne Marc , Motte Patrick 
Published: January 2024

Abstract
The Arabidopsis splicing factor serine/arginine-rich 45 (SR45) contributes to several biological processes. The sr45-1 loss-of-function mutant exhibits delayed root development, late flowering, unusual numbers of floral organs, shorter siliques with decreased seed sets, narrower leaves and petals, and altered metal distribution. SR45 bears a unique RNA recognition motif (RRM) flanked by one serine/arginine-rich (RS) domain on both sides. Here, we studied the function of each SR45 domains by examining their involvement in: (i) the spatial distribution of SR45; (ii) the establishment of a protein–protein interaction network including spliceosomal and exon–exon junction complex (EJC) components; and (iii) the RNA binding specificity. We report that the endogenous SR45 promoter is active during vegetative and reproductive growth, and that the SR45 protein localizes in the nucleus. We demonstrate that the C-terminal arginine/serine-rich domain is a determinant of nuclear localization. We show that the SR45 RRM domain specifically binds purine-rich RNA motifs via three residues (H101, H141, and Y143), and is also involved in protein–protein interactions. We further show that SR45 bridges both mRNA splicing and surveillance machineries as a partner of EJC core components and peripheral factors, which requires phosphoresidues probably phosphorylated by kinases from both the CLK and SRPK families. Our findings provide insights into the contribution of each SR45 domain to both spliceosome and EJC assemblies.