bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Herpes Simplex Virus (Types 1 and 2), Varicella-Zoster Virus, Cytomegalovirus, and Human Herpesvirus 6 and 7 DNA in Cerebrospinal Fluid of Middle Eastern Patients with Encephalitis
Authors: Ibrahim Ali I. , Obeid Michel T. , Jouma Muhidien J. , Roemer Klaus , Mueller-Lantzsch Nikolaus , Gärtner Barbara C. 
Published: August 2005

ABSTRACTHSV-1 DNA was detected in 32 (30%) of 106 cerebrospinal fluid samples from patients with encephalitis. Cytomegalovirus, varicella-zoster virus, and human herpesvirus 6 (HHV-6) DNAs were each detected in three patients (3%); herpes simplex virus type 2 (HSV-2) and HHV-7 PCRs were negative. HSV detection was associated with seizure (P= 0.02), especially focal seizure (P= 0.0002), and pathological computed tomography (P= 0.02) with focal lesions (P= 0.0004).
Multilocus Sequence Typing of
Klebsiella pneumoniae
Nosocomial Isolates
Authors: Diancourt Laure , Passet Virginie , Verhoef Jan , Grimont Patrick A. D. , Brisse Sylvain 
Published: August 2005

ABSTRACT

A multilocus sequence typing (MLST) scheme was developed for
Klebsiella pneumoniae
. Sequences of seven housekeeping genes were obtained for 67
K. pneumoniae
strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of
K. pneumoniae
isolates.
Dissemination of CTX-M-Type Extended-Spectrum β-Lactamase Genes to Unusual Hosts
Authors: Mugnaioli Claudia , Luzzaro Francesco , De Luca Filomena , Brigante Gioconda , Amicosante Gianfranco , Rossolini Gian Maria 
Published: August 2005

ABSTRACT

A
Citrobacter amalonaticus
and a
Morganella morganii
producing the CTX-M-1 extended-spectrum β-lactamase (ESBL) were isolated from an area where this enzyme is now widespread in
Escherichia coli
. This is the first report of CTX-M-1 in the former species. In both cases the ESBL determinant was possibly acquired by these unusual hosts in vivo, after coinfection with
E. coli
strains carrying conjugative plasmids encoding CTX-M-1.
Comparison between Sequence Analysis and a Line Probe Assay for Testing Genotypic Resistance of Human Immunodeficiency Virus Type 1 to Antiretroviral Drugs
Authors: García-Bujalance S. , de Guevara C. Ladrón , González-García J. , Arribas J. R. , Gutiérrez A. 
Published: August 2005

ABSTRACTThe purpose of this study was to compare a line probe assay (LiPA) with sequence analysis for the detection of mutations conferring resistance to nucleoside and non-nucleoside inhibitors in human immunodeficiency reverse transcriptase and protease inhibitors. The limitations for interpreting LiPA make it unacceptable for routine clinical practice.
Characterization of Prolyl Iminopeptidase-Deficient
Neisseria gonorrhoeae
Authors: Blackmore T. , Hererra G. , Shi S. , Bridgewater P. , Wheeler L. , Byrne J. 
Published: August 2005

ABSTRACT

Prolyl iminopeptidase (PIP) is an enzyme produced by
Neisseria gonorrhoeae
, the detection of which is incorporated into several commercial test panels. In this report we describe two distinct mutations in the
pip
gene which account for the loss of PIP activity.
Isolation and Molecular Typing of
Naegleria fowleri
from the Brain of a Cow That Died of Primary Amebic Meningoencephalitis
Authors: Visvesvara Govinda S. , De Jonckheere Johan F. , Sriram Rama , Daft Barbara 
Published: August 2005

ABSTRACT

Naegleria fowleri
causes an acute and rapidly fatal central nervous system infection called primary amebic meningoencephalitis (PAM) in healthy children and young adults. We describe here the identification of
N. fowleri
isolated from the brain of one of several cows that died of PAM based on sequencing of the internal transcribed spacers, including the 5.8S rRNA genes.
High Seroprevalence of Herpes Simplex Virus Type 2 Infection in French Human Immunodeficiency Virus Type 1-Infected Outpatients
Authors: Andréoletti Laurent , Piednoir Emmanuel , Legoff Jérôme , Brodard Véronique , Beguinot Isabelle , Strady Christophe , Rouger Christine , Piketty Christophe , Si-Mohamed Ali , Kazatchkine Michel Daniel , Malkin Jean-Elie , Bélec Laurent 
Published: August 2005

ABSTRACT

Using commercially available herpes simplex virus (HSV) type-specific serological diagnostic tests, HSV type 2 (HSV-2) antibody prevalence was assessed in two parallel prospective studies including 534 human immunodeficiency virus type 1 (HIV-1)-infected outpatients living in two areas of northern France. In the first cohort of 434 subjects, 223 (51%) individuals demonstrated a positive HSV-2 serological status while 66 (66%) of 100 subjects in the second cohort were seropositive for HSV-2 (51 versus 66%;
P
= 0.08). Among the 223 HSV-2-seropositive subjects identified in the first study cohort, only 22 (10%) had suffered from recurrent anogenital lesions during the past 12 months while 154 (69%) had no clinical history of herpesvirus infection. Our findings demonstrate high proportions of subclinical and undiagnosed HSV-2 infection in HIV-1-infected individuals and suggest that HSV type-specific serological testing in the French HIV-1-infected subpopulation could be an efficient strategy to diagnose clinically asymptomatic HSV-2 infections.
Improved Sensitivity of Human Immunodeficiency Virus Type 2 Subtype B Plasma Viral Load Assay
Authors: Damond Florence , Collin Gilles , Descamps Diane , Matheron Sophie , Pueyo Sophie , Taieb Audrey , Campa Pauline , Benard Antoine , Chêne Geneviève , Brun-Vezinet Francoise 
Published: August 2005

ABSTRACT
We developed a new assay for human immunodeficiency virus type 2 plasma RNA quantification based on a previous format. The new version performed significantly better than the original regarding the detection of subtype B, allowing the detection of 14 out of 36 plasma RNAs in the subtype B-infected patients not detected with the original version.
Long-Term Respiratory Tract Infection with Canine-Associated
Pasteurella dagmatis
and
Neisseria canis
in a Patient with Chronic Bronchiectasis
Authors: Allison Kim , Clarridge Jill E. 
Published: August 2005

ABSTRACT

Pasteurella dagmatis
and
Neisseria canis
were repeatedly isolated from the sputum of a poodle-owning patient with chronic bronchiectasis. Commercially available systems failed to identify these unusual organisms: identification was made by 16S rRNA gene sequencing. Difficulties identifying these and five other canine-associated isolates (
P. dagmatis
[
n
= 2],
Pasteurella canis
[
n
= 2], and
N. canis
[
n
= 1]) are discussed.
Pulmonary
Mycobacterium sherrisii
Infection in a Human Immunodeficiency Virus Type 1-Infected Patient
Authors: Gämperli Andrea , Bosshard Philipp P. , Sigrist Thomas , Brändli Otto , Wildermuth Simon , Weber Rainer , Mueller Nicolas J. 
Published: August 2005

ABSTRACT

We report the first case of a pulmonary infection with
Mycobacterium sherrisii
in a patient with advanced human immunodeficiency virus infection.
Mycobacterium sherrisii
is a newly described nontuberculous mycobacterium related to
Mycobacterium simiae
. Sequencing of the 16S rRNA gene was used for species identification. Treatment and antibiotic susceptibilities are described.
Recurrent early childhood injuries among disadvantaged children in primary care settings
Authors: Braun P A , Beaty B L , DiGuiseppi C , Steiner J F 
Published: August 2005

Objective:To examine differences in social risk factors and health care use between young children with and without recurrent injuries.Design:Retrospective cohort study using administrative claims and medical records. Children with no, one, or more than one injury were compared.Setting:Integrated public healthcare system.Subjects:All children born at Denver Health Medical Center (DHMC) in 1993 who continued care there beyond 15 months of age. Children were followed to 36 months.Outcome measures:Sociodemographic information, service use, injury episodes, cause of injury, and social risk factors.Results:371 injury episodes occurred among 817 children. In the study cohort, 7% had >1 injury episode, 26% had one injury, and 67% had none. Among children with >1 injury episode, 78% had at least one social risk factor compared with 63% of children with one injury and 52% of children with none (p<0.0001). Risk factors for >1 injury included maternal substance abuse (p = 0.0003), maternal age under 18 years (p = 0.04), a primary caregiver who was single (p<0.0001) or mentally ill (p = 0.03), and a history of family violence (p = 0.01). Multiple injury episodes were associated with increased non-injury service use, including primary care visits (p<0.0001), emergency department visits (p<0.0001), and total non-injury encounters (p<0.0001).Conclusions:Children with recurrent injury were more likely to have social risk factors, and used DHMC more frequently, than children with one or no injuries. Children with risk factors for recurrent injury can be identified and injury prevention counseling can be delivered to their families at their multiple visits to the system.
Trip characteristics of vehicle crashes involving child passengers
Authors: Chen I G , Durbin D R , Elliott M R , Kallan M J , Winston F K 
Published: August 2005

Objectives:To describe the trip characteristics of vehicle crashes involving children, and to examine the effect of situational factors on front row seating or inappropriate restraint for young children.Methods:A cross sectional study was conducted on children <16 years in crashes of insured vehicles in 15 US states, with data collected using insurance claims records and a telephone interview. A descriptive analysis of the characteristics of vehicle crashes involving children was performed. Multivariate Poisson regression was used to identify situational factors associated with inappropriate restraint or front row seating.Results:These data suggest that children were traveling in vehicles involved in crashes that occurred under usual driving circumstances—that is, closer to home (60%), on a local road (56%), during normal daytime hours (71%), within areas with relatively lower posted speed limits (76%). Compared with children involved in morning crashes, those in daytime crashes (RR = 1.65, 95% CI 1.13 to 2.49) or in night-time crashes (RR = 1.63, 95% CI 1.09 to 2.67) were more likely to be sitting in the front seat. Children involved in night-time crashes were more likely to be inappropriately restrained (RR = 1.12, 95% CI 1.01 to 1.22) than those in daytime crashes. Children riding with two or more additional passengers were more likely to be inappropriately restrained (RR = 1.12, 95% CI 1.02 to 1.27) than those with no other passengers.Conclusions:Educational initiatives should aim to increase the perception that parents have about the potential crash risk of everyday trips. Some situational characteristics of trips were associated with inappropriate restraint and front row seating behaviors for young children.
United States pedestrian fatality rates by vehicle type
Authors: Paulozzi L J 
Published: August 2005

Objective:To describe the relation between motor vehicle type and the risk of fatally injuring a pedestrian.Design:The risk of killing a pedestrian was measured as the number of pedestrian fatalities per billion miles of vehicle travel by each vehicle type in the US in 2002 as reported by the National Highway Traffic Safety Administration’s Fatality Analysis Reporting System.Interventions:None.Main outcome measures:Rates for each vehicle type by sex, age, and rural/urban roadway type and rate comparisons using relative risks (RR) and 95% confidence intervals (CIs).Results:Passenger cars and light trucks (vans, pickups, and sport utility vehicles) accounted for 46.1% and 39.1%, respectively, of the 4875 deaths, with the remainder split among motorcycles, buses, and heavy trucks. Compared with cars, the RR of killing a pedestrian per vehicle mile was 7.97 (95% CI 6.33 to 10.04) for buses; 1.93 (95% CI 1.30 to 2.86) for motorcycles; 1.45 (95% CI 1.37 to 1.55) for light trucks, and 0.96 (95% CI 0.79 to 1.18) for heavy trucks. Compared with cars, buses were 11.85 times (95% CI 6.07 to 23.12) and motorcycles were 3.77 times (95% CI 1.40 to 10.20) more likely per mile to kill children 0–14 years old. Buses were 16.70 times (95% CI 7.30 to 38.19) more likely to kill adults age 85 or older than were cars. The risk of killing a pedestrian per vehicle mile traveled in an urban area was 1.57 times (95% CI 1.47 to 1.67) the risk in a rural area.Conclusions:Outcomes reflect the ways in which a vehicle’s characteristics (mass, front end design, and visibility) and its degree of interaction with pedestrians affect its risk per mile. Modifications in vehicle design might reduce pedestrian injury. The greatest impact on overall US pedestrian mortality will result from reducing the risk from the light truck category.
Chylous ascites as the main manifestation of left ventricular dysfunction: a case report
Authors: Ridruejo Ezequiel , Mandó Oscar G 
Published: August 2005

Abstract

Background
Ascites is one of the most common complications of liver diseases, even though in 15% of the cases it is related to extrahepatic diseases; 3% are of cardiac nature and they appear associated with signs and symptoms of heart failure.

Case presentation
A 70 year old man was admitted with more than one year history of abdominal distension and a weight gain of 10 kilograms. He is asymptomatic and walks 2000–3000 meters a day without angor or dyspnea. The physical examination shows moderate abdominal distension, with no hepatosplenomegaly or edema, and there is mild jugular vein distension. The studies performed (complete laboratory work up, paracentesis, liver biopsy, echocardiogram, intrahepatic pressure measurements, etc.) showed a chylous ascites related to portal hypertension, and left ventricular dysfunction was the only probable cause found.

Conclusion
Asymptomatic heart dysfunction can mimic liver disease and should be kept in mind as a cause of chylous ascites.
Association study of functional genetic variants of innate immunity related genes in celiac disease
Authors: Rueda B , Zhernakova A , López-Nevot MA , Martín J , Koeleman BPC 
Published: August 2005

AbstractBackgroundRecent evidence suggest that the innate immune system is implicated in the early events of celiac disease (CD) pathogenesis. In this work for the first time we have assessed the relevance of different proinflammatory mediators typically related to innate immunity in CD predisposition.MethodsWe performed a familial study in which 105 celiac families characterized by the presence of an affected child with CD were genotyped for functional polymorphisms located at regulatory regions ofIL-1α, IL-1β, IL-1RN, IL-18, RANTESandMCP-1genes. Familial data was analysed with a transmission disequilibrium test (TDT) that revealed no statistically significant differences in the transmission pattern of the different genetic markers considered.ResultsThe TDT analysis forIL-1α, IL-1β, IL-1RN, IL-18, andMCP-1genes genetic variants did not reveal biased transmission to the affected offspring. Only a borderline association ofRANTESpromoter genetic variants with CD predisposition was observed.ConclusionOur results suggest that the analysed polymorphisms ofIL-1α, IL-1β, IL-1RN, IL-18, RANTESandMCP-1genes do not seem to play a major role in CD genetic predisposition in our population.
Invasive breast cancer following bilateral subcutaneous mastectomy in a BRCA2 mutation carrier: a case report and review of the literature
Authors: Kasprzak Lidia , Mesurolle Benoit , Tremblay Francine , Galvez Maria , Halwani Fawaz , Foulkes William D 
Published: August 2005

Abstract

Background
Primary prevention of breast cancer through prophylactic mastectomy can reduce the risk of malignancy in high-risk individuals. No type of mastectomy completely removes all breast tissue, but a subcutaneous mastectomy leaves more tissue in situ than does a simple mastectomy.

Case presentation
We report a case of invasive breast cancer in a BRCA2-positive woman 33 years after bilateral subcutaneous mastectomy. To our knowledge, only one case of primary breast cancer after prophylactic mastectomy in a BRCA1-positive patient has been reported in the literature and none in BRCA2-positive individuals.

Conclusion
Careful documentation and long follow-up is essential to fully assess the benefits and risks of preventive surgical procedures in BRCA1 and BRCA2 mutation carriers.
Modelling of oedemous limbs and venous ulcers using partial differential equations
Authors: Ugail Hassan , Wilson Michael J 
Published: August 2005

AbstractBackgroundOedema, commonly known as tissue swelling, occurs mainly on the leg and the arm. The condition may be associated with a range of causes such as venous diseases, trauma, infection, joint disease and orthopaedic surgery. Oedema is caused by both lymphatic and chronic venous insufficiency, which leads to pooling of blood and fluid in the extremities. This results in swelling, mild redness and scaling of the skin, all of which can culminate in ulceration.MethodsWe present a method to model a wide variety of geometries of limbs affected by oedema and venous ulcers. The shape modelling is based on the PDE method where a set of boundary curves are extracted from 3D scan data and are utilised as boundary conditions to solve a PDE, which provides the geometry of an affected limb. For this work we utilise a mixture of fourth order and sixth order PDEs, the solutions of which enable us to obtain a good representative shape of the limb and associated ulcers in question.ResultsA series of examples are discussed demonstrating the capability of the method to produce good representative shapes of limbs by utilising a series of curves extracted from the scan data. In particular we show how the method could be used to model the shape of an arm and a leg with an associated ulcer.ConclusionWe show how PDE based shape modelling techniques can be utilised to generate a variety of limb shapes and associated ulcers by means of a series of curves extracted from scan data. We also discuss how the method could be used to manipulate a generic shape of a limb and an associated wound so that the model could be fine-tuned for a particular patient.
Cervical stenosis in a professional rugby league football player: a case report
Authors: Pollard Henry , Hansen Lotte , Hoskins Wayne 
Published: August 2005

Abstract

Background

This paper describes a case of C7 radiculopathy in a professional rugby league player after repeated cervical spine trauma. The report outlines the management of the patient following an acute cervical hyperflexion injury with chiropractic manipulation and soft tissue therapies. It also presents a change in approach to include distractive techniques on presentation of a neurological deficit following re-injury. The clinical outcomes, while good, were very dependent upon the athlete restricting himself from further trauma during games, which is a challenge for a professional athlete.

Case presentation

A 30-year old male front row Australian rugby league player presented complaining of neck pain after a hyperflexion and compressive injury during a game. Repeated trauma over a four month period resulted in radicular pain. Radiographs revealed decreased disc height at the C5-C6 and C6-C7 levels and mild calcification within the anterior longitudinal ligament at the C6-C7 level. MRI revealed a right postero-lateral disc protrusion at the C6-C7 level causing a C7 nerve root compression.

Conclusion

Recommendations from the available literature at the present time suggest that conservative management of cervical discogenic pain and disc protrusion, including chiropractic manipulation and ancillary therapies, can be successful in the absence of progressive neurological deficit. The current case highlights the initial successful management of a football athlete, and the later unsuccessful management. This case highlights the issues involvement in the management of a collision sport athlete with a serious neck injury.
Spasmolytic and antidiarrhoeal properties of the Yucatec Mayan medicinal plant Casimiroa tetrameria
Authors: Heinrich Michael , Heneka Bilkis , Rimpler Horst , Ankli Anita , Sticher Otto , Kostiza Thomas 
Published: August 2005

Abstract
The Maya of the Yucatán peninsula commonly use the leaves of Casimiroa tetrameria for treating gastrointestinal disorders, notably diarrhoea and dysentery, as well as gastrointestinal cramps. The phytochemical investigation resulted in the isolation of 13 compounds: eight polymethoxylated flavonoids (two as minor components with a main constituent), four flavonoid glycosides and one furanocoumarin. In this study we used two well-established models in order to assess the gastrointestinal effects of C. tetrameria extracts and isolated compounds: the USSING-chamber, a pharmacological model for diarrhoea, and the isolated guinea pig ileum, a model for modulatory effects on ileum contraction. Extracts and the class of polymethoxylated flavonoids showed strong inhibitory effects in both models, which provides ex-vivo evidence for the use of this botanical drug in the treatment of several gastrointestinal problems, most notably diarrhoea. The crude extract, polymethoxylated flavonoid-rich fractions and the polymethoxylated flavonoids tested showed prominent antisecretory activity. Polymethoxylated flavonoid-rich fractions also inhibited the histamine-induced contractions in the guinea pig model. The effects are not due to a single compound, but to a large number of structurally related compounds that all contribute to the effect.
Cytotoxic activity and effect on nitric oxide production of tirucallane-type triterpenes
Authors: Chávez Ibeth Oviedo , Apan Teresa Ramírez , Martínez-Vázquez Mariano 
Published: August 2005

Abstract
Hexane extract from the bark of Amphipterygium adstringens, as well as its principal constituents, masticadienonic acid (1) and 3α-hydroxymasticadienolic acid (2), inhibited the growth of five human cancer cell lines. Derivatives of 1, namely 24,25S-dihydromasticadienonic acid (3) and masticadienolic acid (4), were also evaluated. The results showed that both 3 and 4 had greater activity than 1 on colon cancer cell lines. The effects of 1–4 on the production of nitric oxide (NO) from both resting and lipopolysaccharide-activated macrophages were determined. It was found that 1, 2 and 4 caused an increase in NO release from resting macrophages; in lipopolysaccharide-activated macrophages, only 2 and 4 caused an increase in NO production.
Role of monocarboxylic acid transporters in the cellular uptake of NSAIDs
Authors: Choi Jun-Shik , Jin Ming Ji , Han Hyo-Kyung 
Published: August 2005

Abstract
The present study investigated the cellular uptake mechanism of non-steroidal anti-inflammatory drugs (NSAIDs) in Caco-2 cells. Diflunisal, diclofenac, ketoprofen and naproxen exhibited a strong inhibition effect on the cellular uptake of [14C]-benzoic acid in Caco-2 cells with IC50 values of 0.05–0.44 mm. The inhibition of naproxen and ketoprofen against the membrane transport of [14C]-benzoic acid appeared to be competitive, with Ki values of 0.22 and 0.38 mm, respectively. The membrane permeability of naproxen and ketoprofen was concentration dependent, implying that the cellular uptake pathway of ketoprofen and naproxen was saturable at the higher concentration. Furthermore, the cellular accumulation of ketoprofen was significantly reduced in the presence of benzoic acid and l-lactic acid, two known substrates of monocarboxylic acid transporter 1 (MCT1). These results suggest that MCT1 contributes at least in part to the carrier-mediated transport of NSAIDs containing a carboxylic acid moiety across the apical membrane in Caco-2 cells.