bluuebunny/crossref_metadata_2025_split huggingface dataset:
#75 : Mechanisms and Active Ingredients of Chinese Herbal Medicine on Ameliorating Endometriosis-Associated Pain
Authors: Lin Yuezhen , Wang Chi Chiu 
Published: January 2024

Background and Aims: Chinese herbal medicines (CHMs) have been effectively used to treat the endometriosis-associated pain (EAP), but its pharmacological and molecular mechanism remains unclear. In this study, we aim to use a network pharmacology-based approach to identify the potential therapeutic targets and active ingredients of CHM for EAP, and to verify their pharmaceutical actions on an experimental mouse model. Method: Pharmacological networks of 3 common CHM formulae for endometriosis (HXXYF, SFZYT and GXZYT) were built by targets collated from public databases, and the enrichment molecular pathway analysis was applied to select the potential pharmacological targets and CHM active ingredients. Pain assessments on the experimental mouse model were performed to evaluate efficacy of the CHM formulae and potential active ingredients. RT-PCR and immunostaining in endometriotic lesions were used to confirm the expression of the potential targets after treatment. Results: ‘Inflammatory mediator regulation of TRP channels’ was a major molecular pathway with highest significance in pharmacological network and enrichment analysis. In mouse, 2 CHM formulae, HXXYF and SFZYT, significantly reduced pain responses, while pain-related inflammatory mediators, IL1[Formula: see text] and PGE2, were significantly decreased after treatment. Meanwhile, densities of nerve fibers in lesions were significantly lower as well, with significantly lower expressions of TRPA1 and TRPV1. 3 CHM ingredients (baicalein, paeoniflorin and quercetin) with highest degree in pharmacological network were then selected for in-depth investigation. However, only paeoniflorin significantly reduced stimuli-evoked and non-stimuli-evoked pain in mice, along with effective reduction in all above parameters. Conclusion: Our study demonstrated multi-targets effects of CHM on EAP relief through inflammatory and neuropathic pathways, which supports CHM as alternative or complementary therapy for EAP. The active ingredients from CHM may provide a novel drug development and approach for EAP treatment.
#95 : Analysis of the Incidence of and Risk Factors for Chronic Endometritis Recurrence in Infertile Women
Authors: Shiobara Keisuke , Kuroda Keiji , Ishiyama Shunsuke , Nakao Kazuki , Moriyama Azusa , Horikawa Takashi , Takamizawa Satoru , Nakagawa Koji , Sugiyama Rikikazu 
Published: January 2024

Background and Aims: Chronic endometritis (CE) is an inflammatory condition of the endometrium which is related to repeated implantation failure. Recovery from CE can increase opportunities for successful pregnancy; however, some of them remain incapable of conceiving or have a miscarriage and concern CE recurrence. The aim of this study is to identify the incidence rates of and risk factors for the recurrence of CE in infertile women. Method: The study population consisted of 1,897 infertile women from a hospital specializing in reproductive medicine who recovered from CE between December 2018 and August 2021. Among the 152 women (13.0%) who did not conceive or experienced pregnancy loss, 105 consecutive women who underwent repeat endometrial biopsy for CD138 immunostaining and endometrial bacterial culturing within 18 months from CE recovery were recruited. Thereafter, patients with and without CE recurrence were compared. Results: The total recurrence rate of CE was 29.5% (31 women). Multivariable logistic regression analysis to determine risk factors for CE recurrence revealed that hysteroscopic surgery (odds ratio [OR], 0.10; 95% confidence interval [CI], 0.02–0.56) and pregnancy loss (OR, 4.13; 95% CI, 1.31–13.05) were significantly associated with decreased and increased CE recurrence rates, respectively. Also, re-examination with CD138 immunostaining after 16–18 months (OR, 9.75; 95% CI, 1.47–64.64) was significantly associated with increased CE recurrence rates. Among 40 patients without a history of hysteroscopic surgery and pregnancy loss, the cumulative CE recurrence rates after 6, 12, and 18 months were 12.5%, 23.3%, and 30.0%, respectively (Graph 1). Conclusion: We recommend re-examination with endometrial CD138 immunostaining in patients with pregnancy loss or long-term infertility during fertility treatment. Hysteroscopic surgery without antibiotic therapy for CE associated with intrauterine abnormalities is also recommended.
#213 : Causal Associations Between Different Plasma Lipid and Apolipoprotein Parameters with the Risk of Endometriosis: A Two-Sample Mendelian Randomization Study
Authors: Yang Shuhan , Zhang Yaoyao , Hu Ying , Guo Weijie 
Published: January 2024

Background and Aims: Endometriosis is a chronic gynecological disease with a high prevalence among reproductive-aged women. Several studies have investigated the causal effect of different plasma lipid and apolipoprotein parameters on the occurrence and progression of endometriosis. However, the results of these studies remained controversial. Herein, this study aims to analyze the causal associations of genetically predicted levels of different blood lipid and apolipoprotein traits with the risk of endometriosis using a two-sample Mendelian randomization (MR) approach. Methods: The single-nucleotide polymorphisms (SNPs) of exposures which indicate the genetic variants were used as instrumental variables (IVs). We assessed the correlation between each blood lipid and apolipoprotein parameter and the occurrence of endometriosis from various cohorts of European ancestry, and the SNPs of them were obtained from the genome-wide association study (GWAS) from the IEU GWAS database. The causal effects were estimated through the inverse-variance weighted method (IVW). The sensitivity examinations of each result after the MR analysis were performed to detect if any heterogeneity and pleiotropy existed. Besides, sub-analyses were performed to provide more detailed information about the actual associations. Results: Our results demonstrated that cholesterol in chylomicron (CM) and extremely large very low-density lipoprotein (VLDL) was causally linked to higher odds of endometriosis, while high-density lipoprotein (HDL) cholesterol showed the opposite correlation. No evidence was detected on the causal relations of low-density lipoprotein (LDL) cholesterol, VLDL cholesterol, triglyceride (TG), apolipoprotein A1 and apolipoprotein B with endometriosis. Neither pleiotropy nor heterogeneity was found in our study. In addition, subgroup analyses about subtypes of endometriosis suggested the causal association of several serum lipids with peritoneal endometriosis. Conclusion: Our results suggested that dysregulated lipid metabolism is causally associated with the occurrence of endometriosis, especially peritoneal endometriosis. Our findings provided genetic insights on potential novel strategies in the primary prevention and treatment of endometriosis.
#219 : Comparative Efficacy and Safety of Interventions for Pain and Quality of Life in the Treatment of Endometriosis: A Network Meta-Analysis
Authors: Gibbons Tatjana , Leonardi Mathew , Stocking Katie , Vail Andy , Kuan Kevin , Lunky Isis , Liu Marina , Sekhon Jasleen , Schreurs Anneke , Shamsa Aiat , Ong Joz , Jung Albert , Cave Adele , Amour Mike , Hao Jessie , Yang Guoyan , Jacobson Tal , Hull Louise , Hodgson Ruth , Glanville Elizabeth , Mijatovic Velja , Becker Christian , Condous George , Johnson Neil 
Published: January 2024

Background and Aims: Numerous endometriosis treatments exist, ranging from surgical to traditional medications to complementary and alternative medicines (CAMs). The ranking of treatments across management categories remains unknown. To address this research gap, this network meta-analysis (NMA) aims to rank endometriosis treatments in terms of efficacy and safety for endometriosis-related pain and quality of life. Method: A systematic literature search and fixed effects NMA was conducted. Eight databases were searched from inception to August 2022. All randomised control trials (RCTs) in women with surgically confirmed endometriosis were considered. Results: After screening 8,668 studies, 165 RCTs were included, consisting of 26,762 participants, investigating 48 medical, 13 CAMs and 16 surgical interventions. Preliminary analysis of the CAMs interventions (9 RCTs, n=522) suggested acupuncture (SMD -1.96; 95%CI: -2.63, -1.29), olive oil (SMD -1.68; 95%CI: -2.69, -0.67), fatty acid supplements (SMD -1.48; 95% CI: -2.15, -0.81), contraception + Chinese medicines (SMD -1.28; 95%CI: -2.39, -0.16), Chinese medicine (SMD -0.91, 95%CI: -1.78, -0.04) and vitamin C&E (SMD -0.38; 95%CI -0.89, 0.13) demonstrated a reduction in pain compared to placebo. There was insufficient evidence to detect a difference in pain using Vitamin D compared to placebo (SMD 0.09 95% CI: -0.55, 0.73). Complete results, including the individual and combined categories within a Bayesian framework and intervention ranking, will be available for ASPIRE 2023. Conclusion: This is the first NMA to compare the evidence of all endometriosis treatments. The findings have the potential to guide treatment decisions and guideline development.
#222 : Exosomes Derived of Menstrual Blood Stem Cells Suppress IUA Endometrial Fibrosis Through P65-UBR4-YAP Pathway
Authors: Tan Jichun , Qi Jiarui , Zhang Xudong 
Published: January 2024

Background and Aims: IUA is the second leading cause of secondary infertility in women, and it is urgent to find a new therapeutic method to achieve functional regeneration of endometrial. We previously found that menstrual blood stem cells (MenSCs) transplantation could improve endometrial fibrosis and restore fertility in IUA rats through exosomes (MenSCs-exos). We identified UBR4 as an E3 ubiquitin ligase that was abundantly enriched in MenSCs-exos by mass spectrometry sequencing, however, the role of UBR4 in MenSCs-exos treatment of IUA and the potential molecular mechanism remains unclear. Method: IUA rats and endometrial stromal cells (EndoSCs) of IUA patients were treated with knock-down UBR4 exosomes (EXOUBR4−) and normal exosomes (EXO). HE staining and Masson staining were used to evaluate endometrial morphology and fibrosis area. The expression levels of fibrotic indicators COL I and CTGF were detected. The molecular mechanisms by which UBR4 in MenSCs-exos alleviate EndoSCs fibrosis were then explored using immunofluorescence, western blot, and co-immunoprecipitation. The molecular mechanism of transcription factor P65 regulating the expression of UBR4 were explored using ChIP and dual luciferase. Results: MenSCs-exos could significantly restore the endometrial morphology of IUA rats, increase endometrial thickness and number of glands, and reduce the endometrial fibrosis area, however reducing the content of UBR4 in MenSCs-exos would alleviate its therapeutic effect on IUA rats. UBR4 in MenSCs-exos reduced the expression levels of COL I and CTGF through significantly decreasing the expression level of YAP by specifically ubiquitin-degrading YAP. In addition, P65 could bind to the UBR4 promoter region to transcriptionally promote the expression level of UBR4 in MenSCs. Conclusion: This study clarified that UBR4, as the key molecule in repairing IUA fibrotic endometrium by MenSCs-exos, could inhibit endometrial fibrosis by ubiquitin-degrading YAP, while transcription factor P65 could promote the expression level of UBR4 in MenSCs by directly binding to the UBR4 promoter region.
#265 : Oocyte Outcome After Laparoscopy Cyst Drainage Procedure Before IVF Stimulation in Endometriosis Patients
Authors: Susanto Cynthia Agnes , Syahbana Luky Satria , Lie Vellyana , Wiweko Budi 
Published: January 2024

Background: Endometriosis is the presence of endometrial-like tissue outside the uterine cavity. In late years, in order to control endometriosis, we often performed cystectomy either laparoscopy or laparotomy. However, cystectomy done in endometriosis patient undergoing IVF can decrease the AMH also the quality of the oocytes, thus cyst drainage procedures (with the insertion of hemostate agent) was considered as a replacement for cystectomy in endometriosis patients before they begin the IVF stimulations. Aims: To see the effect of laparoscopic cyst drainage with the insertion of an absorbable hemostate towards the quality of the oocytes in endometriosis patient Method: A retrospective cohort study took place at Center of Reproductive Medicine and Andrology Bocah Indonesia in Primaya Hospital, Tangerang between January 2021 and December 2022. Patients with endometriosis cysts before IVF stimulations were all included. Excluded were those declining to participate and with incomplete case notes. All of the subjects used ultra-long GnRH agonist protocols. A medical committee approved the study and all women provided written informed consent. Results: 18 patients were included in the analysis (11 undergoing laparoscopy cyst drainage and insertion absorbable hemostatic agent’s vs 7 undergoing only cystectomy). There was a statistically significant difference in AMH values in patients with cystectomy vs drainage with an absorbable hemostatic agent in AMH values 1.002 ± 0.76 to 0.77 ± 0.76 vs 1 (0.30 – 3.31) to 1 (0.30 – 4.00) (p=0.003 vs p =0.678). Among those, there was no significant difference between cystectomy and drainage with the maturation of oocyte (p=0.231 vs p=0.381). On the other side, there was a significant difference between cyst drainage and cystectomy resulting in good embryos in cleavage (p=0.038). One of the patients has a naturally conceive pregnancy after a cyst drainage procedure. Conclusion: Endometriosis with laparoscopic cyst drainage procedure can maintain AMH level, reduce inflammation, and proliferation of lesions so it can maintain better quality of oocytes.
#296 : Identification and Validation of Two Novel Therapeutic Targets for Endometriosis with Artificial Intelligence (AI)
Authors: Wang Chi Chiu , Pun Frank W , Liu Bonnie Hei Man , Lin Yuezhen , Ren Feng , Zhavoronkov Alex 
Published: January 2024

Background and Aims: It is urgent need to develop new-effective and well-tolerable medical treatment for endometriosis, but normal drug development is tedious, costly and time-consuming. Artificial intelligence (AI) is a powerful tool to drive discovery of novel druggable targets. Here we aim to use AI to identify novel therapeutic targets for endometriosis treatment. Method: Targets associated with endometriosis were prioritized by PandaOmics (a well-established AI-driven target identification platform). Expression levels of druggable targets were tested in human endometriotic lesions. Functional characterizations of the targets were performed by in-vitro and in-vivo endometriosis models. Viability, proliferation and apoptosis were examined. Results: Analysis of 11 public endometriosis bulk transcriptomics databases revealed 2 lists of high confidence and novel druggable targets. Tow novel targets ENDO01 and ENDO02 were selected based on ranking in PandaOmics, consistency of significant dysregulated expression across comparisons, as well as literature evidence relevant to disease-driving mechanisms. Differential expressions were confirmed in human endometriosis tissues. siRNA targeting ENDO01 and ENDO02 significantly reduced cell viability and proliferation, as well as enhanced apoptosis in endometriotic cells in-vitro; and significantly decreased lesion volume and weight with reduced proliferative and enhanced apoptosis in-vivo. Conclusion: Two novel targets for endometriosis were identified with AI and validated by in-vitro and in-vivo experiments within a short period. The study demonstrated how AI could speed up the novel target discovery process from years to months. For diseases remaining incurable or with high morbidity, the application of AI in target discovery could be a new therapeutic regimen that benefits patients.
#343 : Social Aspects Affecting Dysmenorrhea and Adenomyosis Women in Indonesia
Authors: Putrinadia Aninditya V , Melinawati Eriana , Budihastuti Uki Retno 
Published: January 2024

Background and Aims: Dysmenorrhea is a common gynecological disorder occurs in women during their reproductive years, especially in women with adenomyosis and it will change daily function which have negative impact on social life. The high prevalence of menstrual disorder requires appropriate prevention and treatment efforts by recognizing the risk factors and found coping strategies and pain acceptance which can improve social aspects affecting dysmenorrhea and adenomyosis women. Method: This study is conducted in three general hospitals in Indonesia using retrospective data with determined inclusion and exclusion criterias. We used patient medical record with dysmenorrhea and adenomyosis. We analyze their risk factors and outcomes of social aspect in affecting dysmenorrhea and adenomyosis women using statistic program with significant p value [Formula: see text] 0.05. Results: In the last three years 250 patients came to the hospital with chief complaints of abdominal pain especially when their periods. Some of them diagnosed with adenomyosis with or without abnormal uterine bleeding and 160 of them have had surgery with histopathological results showing adenomyosis tissue. All patients who met the inclusion criteria were analyzed further using the questionnaires to find out more about social factors that affect psychological condition of patients with adenomyosis. This research is still ongoing. Conclusion: We can conclude that more than 50% patients with dysmenorrhea is caused by adenomyosis, and they have psychological problems because of that with any significant factors which affect those social aspects in dysmenorrhea and adenomyosis women still analyzed.
#123 : Caveolin-1 Function and Regulation in Mouse Uterus During Early Pregnancy and Under Human In Vitro Decidualization
Authors: Zhuo Song 
Published: January 2024

Background and Aims: Endometrial decidualization is a step in establishing and maintaining pregnancy. Senescence was considered to accompany with decidualization. Failure to clear senescence during decidualization could cause pregnancy abnormalities. Caveolin-1, as a scaffold protein, play a role in senescence, but the expression, regulation and function were not very clear in uterine endometrium. Here, we investigated the profile of Caveolin-1 in mouse and human decidualization to tease out the potential relationship among the Caveolin-1, senescence and decidualization. Method: ICR strain mice and immortalized human endometrial stromal cells (ESCs) were used. Immunofluorescence, Western-Blot, Real-time PCR were used to detect the levels of molecules. Results: In endometrium of mice during early pregnancy, the expression of Caveolin-1 gradually decreased in stroma and epithelium before embryo implantation. However, the level of Caveolin-1 converts in stromal cell after implantation. Estrogen could stimulate expression of Caveolin-1 in epithelium and progesterone could up-regulate level of Caveolin-1 in endometrial stromal cells. In vitro, expression of Caveolin-1 was up-regulated during decidualization in mESCs, while the level of Caveolin-1 was reduced during decidualization in hESCs. siRNA of Caveolin-1 could promote the expression of IGFBP-1 (decidualization biomarker) and senescence in hESCs, while over-expression of Caveolin-1 had the opposite results. In mESCs, Caveolin-1 seems to play a different role in decidualization, over-expression of Caveolin-1 could promote decidualization and senescence in mESCs, but siRNA depressed it. Blastocysts-derived TNF and hCG stimulated Caveolin-1 in mice and depressed it in human decidual cells, respectively. Caveolin-1 levels are also regulated by high glucose and insulin. Conclusion: Caveolin-1 play a role in ESCs decidualization, but the mode of action was different between species. A low level of Caveolin-1 will benefit for human decidualization, disorder of Caveolin-1 could impair decidualization.
#288 : Comparison of OVOIL and OVOIL HEAVY in the Clinical IVF Laboratory on Fertilization, Blastocyst Utilization and Transfer Outcomes
Authors: Kathryn Gurner H , Kelley R.L , Lee Y.S , Gardner D.K 
Published: January 2024

Background and Aims: OVOIL HEAVY (Hv-OIL, Vitrolife) is a new synthetic oil, which compared to a paraffin oil OVOIL, has increased viscosity which may benefit handling, and provide osmolality and pH stabilisation. Being synthetically derived, Hv-OIL may reduce risk of impurities/contaminants. The manufacturer reports that Hv-OIL improves mouse and human embryo development 1 . The aim of this study was therefore to compare the effect of Hv-OIL vs OVOIL on IVF/ICSI cycle outcomes. Method: OVOIL and Hv-OIL were used across alternating fortnights for all patients in one laboratory between June 2022 and March 2023 (n=1,116 patients). Donor-recipient, egg thaw and egg freeze cycles were excluded. Embryos were cultured in G-TL media (Vitrolife) under 6.5% CO2, 5% O2 in an Embryoscope+. Results: Hv-OIL (n=554) had no effect on fertilisation rate compared to OVOIL (n=562) following either IVF (53.9% vs 55.1%) or ICSI (61.2% vs 60.5%). Utilisation rate per 2PN was similarly unaffected by oil type (51.2%± 0.01 vs 48.2%± 0.02), and this was unchanged by method of insemination. Hv-Oil had no impact on blastocyst development rate compared to OvOil (64.2 vs 61.5%), and the number of top-quality blastocysts (Gardner grade 4/5AA) per 2PN was not different (14.5% vs 16.6%). Positive hCG (46.52% vs 50.1%), fetal heart (27% vs 32%) and fetal sac (31% vs 36%) rate from fresh, single Day 5 transfers was not different between Hv-Oil (n=210 ETs) and OvOil (n=230 ETs) respectively. Osmolality of culture media in Embryoscope+ slides was not different following 7 days of incubation in either oil. Conclusion: There were no observed differences in fresh cycle outcomes with use of Hv-OIL as an oil overlay. However, the impact of Hv-OIL on live birth and FET outcomes, specific patient populations or use in different types of culture systems remains to be evaluated.