bluuebunny/crossref_metadata_2025_split huggingface dataset:
The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity
Authors: Zhang Min , Lv Lilei , Luo Huaye , Cai Hongming , Yu Lingxue , Jiang Yifeng , Gao Fei , Tong Wu , Li Liwei , Li Guoxin , Zhou Yanjun , Tong Guangzhi , Liu Changlong 
Published: November 2023

AbstractAfrican swine fever virus (ASFV) is a highly contagious and deadly virus that leads to high mortality rates in domestic swine populations. Although the envelope protein CD2v of ASFV has been implicated in immunomodulation, the molecular mechanisms underlying CD2v-mediated immunoregulation remain unclear. In this study, we generated a stable CD2v-expressing porcine macrophage (PAM-CD2v) line and investigated the CD2v-dependent transcriptomic landscape using RNA-seq. GO terms enrichment analysis and gene set enrichment analysis revealed that CD2v predominantly affected the organization and assembly process of the extracellular matrix. Wound healing and Transwell assays showed that CD2v inhibited swine macrophage migration. Further investigation revealed a significant decrease in the expression of transcription factor early growth response 1 (EGR1) through inhibiting the activity of extracellular signal-regulated kinase 1 and 2 (ERK1/2). Notably, EGR1 knockout in swine macrophages restricted cell migration, whereas EGR1 overexpression in PAM-CD2v restored the ability of macrophage migration, suggesting that CD2v inhibits swine macrophage motility by downregulating EGR1 expression. Furthermore, we performed chromatin immunoprecipitation and sequencing for EGR1 and the histone mark H3K27 acetylation (H3K27ac), and we found that EGR1 co-localized with the activated histone modification H3K27ac neighboring the transcriptional start sites. Further analysis indicated that EGR1 and H3K27ac co-occupy the promoter regions of cell locomotion-related genes. Finally, by treating various derivatives of swine macrophages with lipopolysaccharides, we showed that depletion of EGR1 decreased the expression of inflammatory cytokines including TNFα, IL1α, IL1β, IL6, and IL8, which play essential roles in inflammation and host immune response. Collectively, our results provide new insights into the immunomodulatory mechanism of ASFV CD2v.
Agent-based cloud simulation model for resource management
Authors: Dong Dapeng 
Published: November 2023

AbstractDriven by the successful service model and growing demand, cloud computing has evolved from a moderate-sized data center consisting of homogeneous resources to a heterogeneous hyper-scale computing ecosystem. This evolution has made the modern cloud environment increasingly complex. Large-scale empirical studies of essential concepts such as resource allocation, virtual machine migration, and operational cost reduction have typically been conducted using simulations. This paper presents an agent-based cloud simulation model for resource management. The focus is on how service placement strategies, service migration, and server consolidation affect the overall performance of homogeneous and heterogeneous clouds, in terms of energy consumption, resource utilization, and violation of service-level agreements. The main cloud elements are modeled as autonomous agents whose properties are encapsulated. The complex relationships between components are realized through asynchronous agent-to-agent interactions. Operating states and statistics are displayed in real time. In the evaluation, the efficiency of the simulator is studied empirically. The performance of various resource management algorithms is assessed using statistical methods, and the accuracy of server energy consumption models is examined. The results show that agent-based models can accurately reflect cloud status at a fine-grained level.
DB3 from Antarctic lichen inhibits the growth of B16F10 melanoma cells in vitro and in vivo
Authors: Choi Seyeon , Kim Huiji , Shin Seong-Ah , Kim Moonsu , Moon Sun Young , Kim Minji , Lee Seulah , Lee Jun Hyuck , Park Hyun Ho , Youn Ui Joung , Lee Chang Sup 
Published: November 2023

AbstractMalignant melanoma is a fatal disease with an increasing global incidence. Despite numerous studies focused on anti-cancer drugs, a variety of side effects of cancer treatment remain challenging. Thus, there is a pressing need to identify novel anti-cancer agents with minimal cytotoxicity and side effects. DB3 (1,3,7,9-tetrahydroxy-2,8-dimethyl-4,6-di[ethanoyl]dibenzofuran) is a member of the dibenzofuran family and is extracted from Ramalina terebrata (Antarctic lichen). We investigated if DB3 exerted an antitumor effect on B16F10 melanoma cells. The results revealed that DB3 exerted time- and dose-dependent reduction of cell viability by inducing apoptosis and significantly suppressing cell proliferation through cell cycle arrest in the G0/G1 phase in B16F10 melanoma cells. Additionally, DB3 impeded the migration and invasiveness of B16F10 cells. Subsequently, we observed that DB3 decreased the expression levels of Cdk4/Cyclin D1 and the phosphorylation of p38, JNK, ERK, and AKT. Furthermore, DB3 decreased melanoma tumor growth in a mouse tumor syngraft model. Based on these findings, we propose that DB3 possesses potential for use as an anti-cancer agent for melanoma treatment.
A real-world case–control study on the efficacy and safety of pulsed field ablation for atrial fibrillation
Authors: Yang Ming , Wang Peng-yu , Hao Ying-lu , Liang Mei , Yu Zi-yang , Li Xi-chen , Li Yan-ping 
Published: November 2023

Abstract
Objective
The primary objective of this study was to evaluate the efficacy and safety of pulsed field ablation in individuals diagnosed with atrial fibrillation.

Methods
A total of 36 patients diagnosed with atrial fibrillation were enrolled in the pulsed field ablation group, while another 36 patients diagnosed with atrial fibrillation were included in the radiofrequency ablation group. Among the study participants, 15 patients in the pulsed field ablation group and 17 patients in the radiofrequency ablation group had persistent atrial fibrillation. Comprehensive comparisons were made between the two groups, including baseline data, underlying diseases, medication usage, intraoperative parameters, and atrial fibrillation recurrence rates at 1, 3, and 6 months during the postoperative follow-up period.

Results
(1) There were no significant differences observed between the two groups concerning baseline data and antiarrhythmic drug usage (P > 0.05); (2) the effective ablation time for both left and right pulmonary veins in the pulsed field ablation group was markedly shorter compared to the radiofrequency ablation group (P < 0.001 for each vein); (3) within the pulsed field ablation group, the number of discharges, catheter operation time, and effective ablation time for the left pulmonary vein were significantly higher than those for the right pulmonary vein (P < 0.05). Conversely, in the radiofrequency ablation group, the number of discharges for the left pulmonary vein was significantly higher than that for the right pulmonary vein (P < 0.05); and (4) when comparing sinus rhythm maintenance at 1, 3, and 6 months postoperatively, no statistically significant differences were noted between the two groups for paroxysmal, persistent, and paroxysmal + persistent atrial fibrillation cases (P > 0.05).

Conclusion
During the 6-month follow-up period, pulsed field ablation demonstrated comparable efficacy to radiofrequency ablation with respect to recurrence rates for both paroxysmal and persistent atrial fibrillation. Moreover, pulsed field ablation exhibited high safety levels, excellent surgical efficiency, and a notably brief learning curve, affirming its viability as a therapeutic option for these conditions.
Translation of patients’ advance directives in intensive care units: are we there yet?
Authors: Baumann Sira M. , Kruse Natalie J. , Kliem Paulina S. C. , Amacher Simon A. , Hunziker Sabina , Dittrich Tolga D. , Renetseder Fabienne , Grzonka Pascale , Sutter Raoul 
Published: November 2023

Abstract
Objectives
This review examined studies regarding the implementation and translation of patients’ advance directives (AD) in intensive care units (ICUs), focusing on practical difficulties and obstacles.

Methods
The digital PubMed and Medline databases were screened using predefined keywords to identify relevant prospective and retrospective studies published until 2022.

Results
Seventeen studies from the United States, Europe, and South Africa (including 149,413 patients and 1210 healthcare professionals) were identified. The highest prevalence of ADs was described in a prospective study in North America (49%), followed by Central Europe (13%), Asia (4%), Australia and New Zealand (4%), Latin America (3%), and Northern and Southern Europe (2.6%). While four retrospective studies reported limited effects of ADs, four retrospective studies, one survey and one systematic review indicated significant effects on provision of intensive care, higher rates of do-not-resuscitate orders, and care withholding in patients with ADs. Four of these studies showed shorter ICU stays, and lower treatment costs in patients with ADs. One prospective and two retrospective studies reported issues with loss, delayed or no transmission of ADs. One survey revealed that 91% of healthcare workers did not regularly check for ADs. Two retrospective studies and two survey revealed that the implementation of directives is further challenged by issues with their applicability, phrasing, and compliance by the critical care team and family members.

Conclusions
Although ADs may improve intensive- and end-of-life care, insufficient knowledge, lack of awareness, poor communication between healthcare providers and patients or surrogates, lack of standardization of directives, as well as ethical and legal concerns challenge their implementation.
Mechanisms of hyponatremia and diabetes insipidus after acute spinal cord injury: a critical review
Authors: Li Lianhua , Guo Yanhui , Chen Chen , Wang Zhonghe , Liu Zhi 
Published: November 2023

AbstractThe incidence of hyponatremia after spinal cord injury was reported to be between 25 and 80%. Hyponatremia can lead to a variety of clinical symptoms, from mild to severe and even life-threatening. Hyponatremia is often associated with diabetes insipidus, which refers to insufficient arginine vasopressin (AVP) secretion or defective renal response to AVP, with clinical manifestations of syndromes such as hypoosmolality, polydipsia, and polydipsia. Recent mechanistic studies on hyponatremia and diabetes insipidus after acute spinal cord injury have been performed in isolation, without integrating the above two symptoms into different pathological manifestations that occur in the same injury state and without considering the acute spinal cord injury patient’s condition as a whole. The therapeutic principles of CSWS and SIADH are in opposition to one another. It is not easy to identify the mechanism of hyponatremia in clinical practice, which makes selecting the treatment difficult. According to the existing theories, treatments for hyponatremia and diabetes insipidus together are contraindicated, whether the mechanism of hyponatremia is thought to be CSWS or SIADH. In this paper, we review the mechanism of these two pathological manifestations and suggest that our current understanding of the mechanisms of hyponatremia and diabetes insipidus after high acute cervical SCI is insufficient, and it is likely that there are other undetected pathogenetic mechanisms.
Glutamate as a new path in discrimination between neuromyelitis optica spectrum disorder and multiple sclerosis
Authors: Rabah Amany Mahmoud , El Sayed El Awady Mohamed , Rashed Laila Ahmed , Elelwany Doaa Abdellatif , Fotouh Al-Shaimaa Mahmoud Aboul 
Published: November 2023

Abstract
Background
Neuromyelitis optica spectrum disorder (NMOSD) used to be considered as a variant of multiple sclerosis (MS), however the recent detection of a highly specific serum biomarkers for NMOSD have made clear that NMOSD is a condition distinct from MS. The aim was to explore the role of serum glutamate level in the discrimination between NMOSD and relapsing remitting (RR) MS patients during and in between relapses. The study comprised two groups; first group, a total of 30 NMOSD patients, they were furtherly subdivided into NMOSD in remission, 15 patients without recent relapses in the last 3 months, NMOSD with relapse, 15 patients with recent relapses in the last 3 months, the second group, 30 definite, RRMS patients, they were further subdivided into RRMS in remission, 15 patients without recent relapses in the last 3 months RRMS with relapse, 15 patients with recent relapses in the last 3 months.

Results
Without relapse, NMOSD patients have higher level of serum glutamate than RRMS patients with (P values = 0.005), a significant difference between EDSS in NMOSD patients and RRMS patients (P = 0.0001), The cut-off value of glutamate serum level between NMOSD in remission and RRMS in remission was > 10.3 μg/mL, yet its level for differentiation between group RRMS in remission and RRMS with relapse was > 12.6 μg/mL.

Conclusion
Glutamate cut-off value might be a reliable tool to discriminate between NMOSD and RRMS.
Bayesian rock-physics inversion using a localized ensemble-based approach — With an application to the Alvheim field
Authors: Spremić Mina , Eidsvik Jo , Avseth Per 
Published: November 2023

We describe, implement, and show the results of a localized ensemble-based approach for seismic amplitude-variation-with-offset (AVO) inversion with uncertainty quantification. Ensembles are simulated from prior probability distributions for fluid saturations and clay content. Starting with continuous saturations and clay content variables, we use depth-varying models for cementation and grain contact theory, Gassmann fluid substitution with mixed saturations, and approximations to the Zoeppritz equations for the AVO attributes at the top-reservoir. The local conditioning to seismic AVO observations relies on (1) the misfit between ensemble simulated seismic AVO data and the field observations in a local partition of the grid/local patch, of inlines/crosslines around the locations where we aim to predict, (2) correlations between the simulated reservoir properties and the data in local patches, and (3) local assessment to avoid unrealistic updates based on spurious correlations in the ensembles. Data from the Alvheim field in the North Sea are used to demonstrate the approach. The influence of the prior information from the well logs in combination with the seismic reflection data indicates the presence of higher oil and gas saturation in the lobe structures of the field and increased clay content at their edges.
DHT and Insulin Upregulate Secretion of the Soluble Decoy Receptor of IL-33 From Decidualized Endometrial Stromal Cells
Authors: Salamon Daniel , Ujvari Dorina , Hellberg Anton , Hirschberg Angelica Lindén 
Published: November 2023

AbstractInterleukin 33 (IL-33) signaling regulates most of the key processes of pregnancy, including decidualization, trophoblast proliferation and invasion, vascular remodeling, and placental growth. Accordingly, dysregulation of IL-33, its membrane-bound receptor (ST2L, transducer of IL-33 signaling), and its soluble decoy receptor (sST2, inhibitor of IL-33 signaling) has been linked to a wide range of adverse pregnancy outcomes that are common in women with obesity and polycystic ovary syndrome, that is, conditions associated with hyperandrogenism, insulin resistance, and compensatory hyperinsulinemia. To reveal if androgens and insulin might modulate uteroplacental IL-33 signaling, we investigated the effect of dihydrotestosterone (DHT) and/or insulin on the expression of ST2L and sST2 (along with the activity of their promoter regions), IL-33 and sIL1RAP (heterodimerization partner of sST2), during in vitro decidualization of endometrial stromal cells from 9 healthy women. DHT and insulin markedly upregulated sST2 secretion, in addition to the upregulation of its messenger RNA (mRNA) expression, while the proximal ST2 promoter, from which the sST2 transcript originates, was upregulated by insulin, and in a synergistic manner by DHT and insulin combination treatment. On the other hand, sIL1RAP was slightly downregulated by insulin and IL-33 mRNA expression was not affected by any of the hormones, while ST2L mRNA expression and transcription from its promoter region (distal ST2 promoter) could not be detected or showed a negligibly low level. We hypothesize that high levels of androgens and insulin might lead to subfertility and pregnancy complications, at least partially, through the sST2-dependent downregulation of uteroplacental IL-33 signaling.
Appropriate leucine-rich α-2 glycoprotein cut-off value for Japanese patients with ulcerative colitis
Authors: Yamazato Masanao , Yanai Shunichi , Oizumi Tomofumi , Eizuka Makoto , Yamada Shun , Toya Yosuke , Uesugi Noriyuki , Sugai Tamotsu , Matsumoto Takayuki 
Published: November 2023

BACKGROUND
It has been suggested that serum leucine-rich α-2 glycoprotein (LRG) could be a novel monitoring biomarker for the assessment of disease activity in inflammatory bowel disease. In particular, the relationship between LRG levels and the endoscopically assessed activity of ulcerative colitis (UC) has become a matter of interest.
AIM
To clarify appropriate LRG cut-off values for the prediction of endoscopic and histologic remission in Japanese patients with UC.
METHODS
This was a cross-sectional, single-center, observational study of Japanese patients with UC. Among 213 patients with UC, in whom LRG was measured from September 2020 to February 2022, we recruited 30 patients for whom a total colonoscopy and measurements of LRG and C-reactive protein (CRP) were performed on the same day. We retrospectively analyzed correlations between the LRG and CRP levels and endoscopic indices, including the Mayo endoscopic subscore and UC endoscopic index of severity.
RESULTS
Correlations between the LRG values and the Mayo endoscopic subscore or UC endoscopic index of severity were significant (r = 0.754, P < 0.0001; r = 0.778, P < 0.0001, respectively). There were also significant correlations between CRP levels and Mayo endoscopic subscore or UC endoscopic index of severity (r = 0.599, P = 0.0005; r = 0.563, P = 0.0012, respectively), although the correlation coefficients were higher for LRG. The LRG cut-off value for predicting endoscopic remission was 13.4 μg/mL for a Mayo endoscopic subscore of 0 [area under the curve (AUC): 0.871; 95% confidence interval (CI): 0.744–0.998], and 13.4 μg/mL for an UC endoscopic index of severity of 0 or 1 (AUC: 0.904; 95%CI: 0.792–1.000).
CONCLUSION
LRG may be a surrogate marker for endoscopic activity in UC, with a cut-off value of around 13.4 μg/mL for endoscopically inactive disease.
Transcatheter embolization for hemorrhage from aberrant testicular artery after partial nephrectomy: A case report
Authors: Youm Juyoun , Choi Min-Jeong , Kim Bong Man , Seo Yumi 
Published: November 2023

BACKGROUND
Arterial bleeding typically involves the renal artery following partial nephrectomy; in this study, we present a case of bleeding originating from the testicular artery that has not been reported in previous studies.
CASE SUMMARY
A 52-year-old man suffered hemorrhage from a perinephric branch of the aberrant left testicular artery after an open nephron-sparing surgery for renal cell carcinoma. Clinical signs of bleeding were manifested by the patient, such as fresh blood drainage from the catheter, decreased hemoglobin levels, and significant vital sign changes. Since computed tomography did not show evidence of active bleeding, transcatheter angiography was conducted to identify the bleeding site. Fluoroscopic spot images confirmed bleeding derived from a perinephric branch of the testicular artery originating from the segmental artery of the left renal artery. Using n-butyl-2-cyanoacrylate, successful transcatheter arterial embolization of the affected branch was performed. Immediately after the embolization procedure, the bleeding ceased, and the patient experienced complete recovery devoid of complications.
CONCLUSION
In patients with postoperative arterial hemorrhage after partial nephrectomy, the testicular artery can be a rare but notable source of bleeding. Accurate bleeding site localization via angiographic evaluation, followed by transcatheter arterial embolization, can be instrumental for safe, prompt, and effective hemostasis.
Glucocorticoid reduction induced chorea in pediatric-onset systemic lupus erythematosus: A case report
Authors: Xu Yan-Qiu , Wang Miao , Zhang Ying 
Published: November 2023

BACKGROUND
Pediatric-onset systemic lupus erythematosus (SLE) is typically more severe than adult-onset SLE, with a higher incidence of nervous system involvement. Chorea is a relatively rare neurological complication reported in 2.4%-7% of SLE patients. In particular, chorea induced by glucocorticoid dose reduction is even rarer. Herein, we report the case of a girl with SLE, who developed chorea during the process of glucocorticoid therapy reduction.
CASE SUMMARY
We describe a 14-year-old girl who was diagnosed with SLE. She was treated with methylprednisolone and rituximab, and her symptoms improved. On the second day after the methylprednisolone dose was reduced according to the treatment guidelines, the patient developed chorea. Her condition improved after adjusting her glucocorticoid regimen.
CONCLUSION
This case is a reminder that extra attention to chorea is required in SLE patients during glucocorticoid dose reduction.
New treatment for gastric duplication cyst: Endoscopic ultrasonography-guided fine-needle aspiration combined with lauromacrogol sclerotherapy: A case report
Authors: Bu Ya-Wei , Han Ruo-Qi , Ma Wen-Qian , Wang Gong-Ning , Er Li-Mian 
Published: November 2023

BACKGROUND
Gastric duplication cysts are very rare disease that are mainly diagnosed by endoscopic ultrasonographic fine-needle aspiration biopsy. In the past, this disease was usually treated with traditional surgery and rarely with minimally invasive endoscopic surgery. However, minimally invasive endoscopic therapy has many advantages, such as no skin wound, organ preservation, postoperative pain reduction, early food intake, fewer postoperative complications, and shorter post-procedure hospitalization.
CASE SUMMARY
We report a case of endoscopic ultrasonography-guided fine-needle aspiration (EUS-FNA) combined with lauromacrogol sclerotherapy for pyloric obstruction due to gastric duplication cysts.
CONCLUSION
EUS-FNA combined with lauromacrogol sclerotherapy provides a new option for the treatment of gastrointestinal duplication cysts.
Immunotherapy in SMARCB1 (INI-1)-deficient sinonasal carcinoma: Two case reports
Authors: Zhang Lu , Gao Ai-Xin , He Yu-Lu , Xu Ming-Jin , Lu Hai-Jun 
Published: November 2023

BACKGROUND
SMARCB1/INI-1 deficient sinonasal carcinoma (SDSC) is a rare subset of sinonasal undifferentiated carcinoma with a poor prognosis. Here, we present two case reports of SDSC patients. We also review the literature on this tumor. This is the first published report of SDSC treatment with immunotherapy.
CASE SUMMARY
Here we present two patient cases of SDSC in which initial consultation and diagnosis were complicated but SDSC was ultimately diagnosed. One patient received a traditional treatment of surgery and adjuvant chemoradiotherapy, while the other patient received additional immunotherapy; the prognoses of these two patients differed. We review previous diagnostic literature reports and SDSC treatments and provide a unique perspective on this rare type of tumor.
CONCLUSION
SDSC is a rare, diagnostically challenging carcinoma with a consistently poor prognosis, early distant metastases, and frequent recurrence. Timely diagnosis and intervention are critical for treatment, for which the standard of care is surgery followed by adjuvant chemoradiotherapy, though immunotherapy may be an effective new treatment for SDSC.