bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virulence of Philippine Entomopathogenic Nematode against Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) Strains
Authors: DUZA GERALD MEDINA , LATINA ROMNICK A , YAP SHERYL A , DALISAY TERESITA U , PINILI MARITA S , CAOILI BARBARA LAVIÑA 
Published: October 2023

Abstract
The control of fall armyworm (FAW), Spodoptera frugiperda, has been a significant concern to the corn and rice in the Philippines. Recent research has focused on developing control strategies using biological controls such as entomopathogenic nematodes. This paper evaluated the efficacy of the Philippine isolates Heterorhabditis indica (HiBSDS, HiMAP, HiPBCB) and Steinernema abbasi (SaMBLB) against two strains of S. frugiperda (J.E. Smith). The Philippine EPN isolates were shown to be pathogenic to S. frugiperda regardless of FAW strains. Among the three isolates under the genus Heterorhabditids, HiPBCB was the most virulent among the test isolates tested against two strains of FAW. The result showed a high significance on the pathogenicity test exposed to all isolates at 24 hpi. The highest LC50 was observed for SaMBLB, corresponding to 36.95 IJs/larva (CS) and 35.92 IJs/larva (RS). This was sufficient to achieve 100% mortality after 48 hrs significantly differed from the three H. indica. On the lethal time (LT50) estimates, infection with H. indica in both strains had no significant differences due to the overlapping fiducial limits. However, both strains had significantly longer lethal times (LT50) when infected with S. abbasi (MBLB). No intraspecific variability was observed in the virulence of the different H. indica isolates. More apparent is the interspecific variability between S. abbasi and H. indica, as the latter exhibited higher virulence activity. This study revealed no pathogenicity variations in strains of S. frugiperda. However, it showed a different level of virulence, LC50, and LT50 between H. indica and S. abbasi.
Adaptation and Validation of the Greek Version of a Short-Form Oral Health Impact Profile and Psychosocial Impact of Dental Aesthetics Questionnaire: The OHIP-14-Gr and PIDAQ-Gr
Authors: Balti Anthoula Spiridoula , Möltner Andreas , Rüttermann Stefan , Gerhardt-Szép Susanne , Giraki Maria 
Published: October 2023

Abstract
Background:Oral health-related quality of life (OHRQoL) is recognized as a significant aspect of health outcomes. The Oral Health Impact Profile (OHIP-14) and the Psychosocial Impact Dental Aesthetics Questionnaire (PIDAQ) are valid instruments that capture different facets of OHRQoL. The OHIP-14 focuseson the effects of oral disorders, whereas the PIDAQ emphasizes the impacts of dental aesthetics on quality of life. The intention of this study was to adapt the OHIP-14 and PIDAQ in a Greek cultural context and to investigate their psychometric properties in all age groups of adults.
Methods:The original English OHIP-14 and PIDAQ questionnaires were translated into Greek, cross-culturally adapted according to the forward-backward technique, pretested, and applied to a validation sample of 280 participants aged 18-79 in dental practice. In total, 59 participants in thevalidation set underwent the procedure after 12 weeks. Internal consistency and reproducibility established reliability. Content, construct, and convergent validity were supported. The questionnaires were also contrasted with sociodemographic variables (age, gender, education, residence).
Discussion:Cronbach´s alpha was 0.94 for the OHIP-14-Gr and 0.95 for the PIDAQ-Gr. Correcteditem-total correlation coefficients ranged from 0.45 to 0.81 for the OHIP-14-Gr and from 0.53 to 0.82 for the PIDAQ-Gr. Average interitemcorrelation coefficients were 0.52 (OHIP-14-Gr) and 0.48 (PIDAQ-Gr). Interclass correlation coefficient(ICC) was 0.73 for theOHIP-14-Gr and 0.86 for the PIDAQ-Gr. Factor analysis with target rotation confirmed the four-dimensional factor structure of the PIDAQ-Gr. Item factor loadings varied between 0.38 and 1.09. Pearson`s correlation coefficients between the subscales and the total scores of the OHIP-14-Gr and PIDAQ-Gr ranged from 0.29 to 0.67,resulting in highly significant correlations for the total scores and subscales of the Greek questionnaires (p < 0.001).
The results attribute good to excellent psychometric properties to the Greek versionsof the PIDAQ and OHIP-14. They indicate reliable and valid tools for the standardized assessment of the psychosocial impact of oral disorders and dental aesthetics among adults. The present study extends the use of these questionnaires to includethe important target group of young adults.
RETRACTED: “Visualization matters” – Stereoscopic visualization of 3D graphic neuroanatomic models through AnaVu enhances basic recall and radiologic anatomy learning when compared with monoscopy
Authors: Yohannan Doris George , Oommen Aswathy Maria , Kumar Amruth S , Devanand S , Resivi Minha , Sajan Navya , Thomas Neha Elizabeth , Anzer Nasreen , Raju Nithin Kadakampallill , Thomas Bejoy , Rajan Jayadevan Enakshy , Govindapillai Umesan Kannavilakom , Harish Pawan , Kapilamoorthy Tirur Raman , Kesavadas Chandrasekharan , Sivaswamy Jayanthi 
Published: October 2023

Abstract

The authors have requested that this preprint be removed from Research Square.
A Novel HOIP Frameshift Variant Alleviates NF-kappaB Signaling and Sensitizes Cells to TNF-induced Death
Authors: Tu Xin , Wang Mengru , Bai Ying , Jiang Dan , Wang Yue , Zhou Yingchao , Zhou Mengchen , Chen Yilin , Yu Chenguang , Wang Xiangyi , Guo Qiang , Zha Lingfeng , Li Qianqian , Cao Zhubing , Wu Jianfei , Shi Shumei , Wang Qing , Xu Chengqi , Kong Xiangdong 
Published: October 2023

Abstract
The linear ubiquitin chain assembly complex (LUBAC) is the sole known complex that can assemble linear ubiquitin chains. It is composed of three distinct subunits: HOIP, SHARPIN, and HOIL-1L, and has a wide range of tissue expression patterns. HOIP, the key component of LUBAC, possesses the capability to form linear ubiquitin linkages. In this study, we report a novel homozygous deletion variant of HOIP that leads to the loss of the catalytic domain of HOIP. The truncated protein could still interact with the other two proteins but was unable to create linear chains. Cells transfected with the truncated protein demonstrated impaired NF-κB activation and MAPK signaling when compared to wild-type cells, as well as sensitivity to TNF-induced death, including apoptosis, necroptosis, and pyroptotic forms of death. This finding provides insight into the genetic range of linear ubiquitination deficiencies in humans and suggests the involvement of cell death in disease pathogenesis.
Effective Kirschner wire fixation for tissue necrosis of open comminuted tibial plateau fracture: A case report and literature review
Authors: Wang Zeyong , Yang Fei , Zhu Yiran , Qin Jian , Yuan Tangbo 
Published: October 2023

Abstract

Background:As a part of the knee joint, the tibial plateau plays a very important role in the weight-bearing function of the knee joint. Tibial plateau fracture is usually caused by severe violent injury. Clinical treatment is mainly to reconstruct the structure of tibial plateau and restore the function of knee joint. The main surgical treatments include traditional open reduction and internal fixation, external fixation, and arthroscopy-assisted minimally invasive techniques, depending on the extent of the tibial plateau fracture and the tissue condition of the wound. For patients with open comminuted tibial plateau fracture with poor tissue condition and not suitable for internal and external fixation, the fixation of Kirschner wires can be used as an option for temporary reconstruction of the tibial plateau structure, which is conducive to the early functional exercise of patients, and is an alternative solution.Case presentation:We present the case of a 72-year-old female patient with an open comminuted tibial plateau fracture who sufferd a severe violent traffic accident injury with tissue necrosis around the wound. We performed autologous skin flap to transplant necrotic skin and used Kirschner wires to temporarily fix the tibial plateau. One year postoperatively, the patient's knee function recovered well without traumatic osteoarthritis.Conclusion：Temporary fixation of the Kirschner wires combined with autologous flap transplantation can provide effective support and restore necrotic skin function for open comminuted fractures with poor wound tissue condition that is not suitable for internal and external fixation therapy.
Clinical and genetic characteristics of CYP2U1-associated hereditary spastic paraplegia in three children from China
Authors: Guo Liqi , Jiang Wei , Lu Guanting , Zhong Min 
Published: October 2023

Abstract
Background and objective
Hereditary spastic paraplegia (HSP) type 56 (SPG56), caused by mutations in CYP2U1, is a rare type of HSP with an autosomal recessive (AR) mode of inheritance. The onset of SPG56 usually occurs early and displays complex symptoms. To date, less than 27 variants have been reported from 25 families across different countries and regions worldwide. This study is the first to describe the clinical manifestations, imaging characteristics, and mutated CYP2U1 loci in 3 children from China.
Methods
CYP2U1 mutations were identified by Trio-WES of the samples obtained from the children clinically diagnosed with HSP. Subsequently, we summarized the clinical presentation, imaging features, and family history and further compared with previously reported cases.
Results
Five novel mutations, namely, c.1032T>A, c.179C>G, c.570(exon2)delG, c.470(exon1)delC, and c.1526A>C(E5), were identified. Two patients carried compound heterozygous mutations derived from their parents with heterozygous mutations. The third child harbored a homozygous mutation, inherited from his parents. Disease onset was infantile in all the patients. Two patients developed motor regression and one displayed an abnormal gait. The patients exhibited varying degrees of delay in cognitive/language development, active tendon reflexes, positive ankle clonus, and increased muscle tone. Cranial and spinal cord magnetic resonance imaging (MRI) revealed abnormalities in all the patients.
Conclusions
This study identified five novel variants in the three patients, and expanded the clinical and molecular spectrum of SPG56. The identified mutant loci were relatively conserved across several species, and the findings provide a basis for subsequent functional validation.
ChatGPT Demonstrates Potential for Identifying Psychiatric Disorders: Application to Childbirth-Related Post-Traumatic Stress Disorder
Authors: Bartal Alon , Jagodnik Kathleen , Chan Sabrina , Dekel Sharon 
Published: October 2023

Abstract
Free-text analysis using Machine Learning (ML)-based Natural Language Processing (NLP) shows promise for diagnosing psychiatric conditions. Chat Generative Pre-trained Transformer (ChatGPT) has demonstrated initial feasibility for this purpose; however, this work remains preliminary, and whether it can accurately assess mental illness remains to be determined. This study examines ChatGPT’s utility to identify post-traumatic stress disorder following childbirth (CB-PTSD), a maternal postpartum mental illness affecting millions of women annually, with no standard screening protocol. We explore ChatGPT’s potential to screen for CB-PTSD by analyzing maternal childbirth narratives as the sole data source. By developing an ML model that utilizes ChatGPT’s knowledge, we identify CB-PTSD via narrative classification. Our model outperformed (F1 score: 0.82) ChatGPT and six previously published large language models (LLMs) trained on mental health or clinical domains data, suggesting that ChatGPT can be harnessed to identify CB-PTSD. Our modeling approach can be generalized to assess other mental health disorders.
A Dental Bleaching Survey on Two Bleaching Systems and Their Effect on Nitric Oxide and Catalase Enzyme in the Gingival Crevicular Fluid
Authors: Wakeel Aliaa Mohamed el , Baz Maha Abdel Salam El , Fadel Mona Mohamed , Moneim Ahmed Esmat Abdel , Aref Ahmed Mostafa , Refai Ibrahim Mahmoud El 
Published: October 2023

Abstract
Objective:
This study was carried out to examine the effect of two in-office bleaching systems by measuring the level of catalase enzyme (CAT) and nitric oxide (NO) in the gingival fluid (GCF) before and after bleaching. In addition, each participant was asked to fill out an online survey to examine their satisfaction with the bleaching procedure.
Materials and Methods:
Thirty-six healthy young participants were selected. They were divided into two groups according to the bleaching system used; Philips Zoom White of 25% H2O2 or Fläsh White Smile of 32% H2O2. Three sessions, 15 minutes each were performed in the same visit for each participant. The (GCF) samples were collected using a sterile periopaper before and after the bleaching session. A survey link was sent to all participants to examine their satisfaction.
Results:
There was a statistically significant increase in (CAT) and (NO) in the (GCF) of the Fläsh group when compared to the Zoom group. Participants reported 94% satisfaction with both bleaching systems. There was no statistically significant difference between the Fläsh and Zoom groups in all participant's answers except in the degree of gingival pain where the number of participants who reported no or mild pain in the Fläsh were greater than those in the Zoom.
Conclusion:
The higher percentage of H2O2 in Fläsh group resulted in the increase of (CAT) and (NO) release in the (GCF). Participants in both groups were equally satisfied.
Clinical relevance:
Risks from dental materials have to be evaluated to prevent endangering human health.
Analysis of Immune-Related and Angiogenesis-Related Genes in Hepatocellular Carcinoma: Implications for Prognosis and Drug Sensitivity
Authors: wang peng , zhao haoran , liu chunxun , zhang yubao 
Published: October 2023

Abstract
In this study, we conducted a comprehensive analysis of immune-related and angiogenesis-related genes (IRAR DEGs) in hepatocellular carcinoma (HCC). We utilized data from the TCGA-LIHC project and performed expression difference and correlation analysis, clinical prognosis analysis, enrichment analysis, correlation analysis with immune cell infiltration, construction of advanced prognostic models, and evaluation of drug sensitivity. Our findings revealed 18 IRAR DEGs associated with both immune response and angiogenesis in HCC. We identified significant expression differences and correlations among these genes. Additionally, we demonstrated their clinical relevance in terms of tumor stage, survival prognosis, and immune cell infiltration. Furthermore, we performed functional enrichment analysis and identified enriched pathways and biological processes. Our study provides valuable insights into the molecular mechanisms and clinical implications of IRAR DEGs in HCC, which may contribute to the development of targeted therapies and precision medicine approaches for HCC patients.
Impact of center volume on in-hospital mortality in adult patients with out‑of‑hospital cardiac arrest resuscitated using extracorporeal cardiopulmonary resuscitation: A secondary analysis of the SAVE-J II study
Authors: Misumi Kayo , Hagiwara Yoshihiro , Kimura Takuya , Hifumi Toru , Inoue Akihiko , Sakamoto Tetsuya , Kuroda Yasuhiro , Ogura Takayuki 
Published: October 2023

Abstract
Background:Recently, patients with out-of-hospital cardiac arrest (OHCA) refractory to conventional resuscitation have started undergoing extracorporeal cardiopulmonary resuscitation (ECPR). However, the mortality rate of these patients remains high. This study aimed to clarify whether a center ECPR volume was associated with the survival rates of adult patients with OHCA resuscitated using ECPR.
Methods: This was a secondary analysis of a retrospective multicenter registry study, the SAVE-J II study, involving 36 participating institutions in Japan. The centers were divided into three groups according to the tertiles of the annual average number of patients undergoing ECPR: high-volume (≥21 sessions per year), medium-volume (11–20 sessions per year), or low-volume (<11 sessions per year). The primary outcome was survival rate at the time of discharge. Patient characteristics and outcomes were compared among the three groups. Moreover, a multivariable-adjusted logistic regression model was applied to study the impact of center ECPR volume.
Results: A total of 1,740 patients were included in this study. The center ECPR volume was strongly associated with survival rate at the time of discharge; furthermore, survival rate was best in high-volume compared with medium- and low-volume centers (33.4%, 24.1%, and 26.8%, respectively; P=0.001). After adjusting for patient characteristics, undergoing ECPR at high-volume centers was associated with an increased likelihood of survival compared to middle- (adjusted odds ratio 0.657; P=0.003) and low-volume centers (adjusted odds ratio 0.983; P=0.006).
Conclusion: The annual number of ECPR sessions was associated with favorable survival rates and lower complication rates of the ECPR procedure.
IRB information: The present study was approved by the institutional review board of Kagawa
University (approval number: 2018-110)
Clinical trial registration: https://center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000041577 (unique identifier: UMIN000036490, Registered 15 April 2019, retrospectively registered)
Comprehensive landscape of the GZM gene family in pan-cancer: Based on large-scale omics research and single-cell sequencing validation
Authors: Li Zitao , Xie Qiqi , Zhao Fuxing , Ren Dengfeng , Liu Zhilin , Shen Guoshuang , Zhao Jiuda 
Published: October 2023

Abstract
The granzyme family plays a crucial role in the immune response against tumor cells. It is involved in inflammation, tumor development, and various survival pathways. However, the precise function of the GZM gene family in a wide range of cancers remains unclear. Through an analysis of cancer genome mapping data, we observed differential expression of the GZM gene family in tumors, which was associated with somatic copy number changes (SCNA) and DNA methylation. Notably, we found a strong positive correlation between the GZM gene family and PD-1 expression in various cancers, suggesting its involvement in tumor immune evasion. To elucidate the function of this gene family, we developed GZMscores, which showed significant differences between tumor and normal tissues across different cancers, and were also correlated with prognosis and clinical features. Pathway analysis revealed close associations between GZMscores and multiple immune and inflammatory pathways. High GZMscores were predictive of poor prognosis in diverse tumors. Our study provides a comprehensive analysis of the GZM gene family and underscores the potential of GZMscores-based cancer immunotherapy. Furthermore, our single-cell pan-carcinoma analysis demonstrated the close relationship between the GZM gene family and pDC_LILRA4 cells and cDC1_CLEC9A cells, suggesting their influence on tumor immunity and recruitment of effector T cells through the modulation of inflammatory factor release from pDC_LILRA4 cells. In TNBC single-cell analysis, we observed widespread expression of the GZM gene family in T cells and NK cells, and a strong correlation with CD8_MAIT-KLRB1, CD8-CXCL13, Bfoc-MKI67, Bmem-MKI67, cDC2-CD207, pDC-LILRA4, ILC1-CD160, ILC1-CX3CR1, ILC1-IFNG, and ILC1-IL32. In summary, our extensive multiomics and single-cell analysis shed light on the function and characteristics of the GZM gene family in different types of cancer. These findings emphasize the potential of harnessing the GZM gene family for patient immunotherapy responses and offer new directions for future immunotherapy. Additionally, our findings present a promising avenue for immunotargeted therapy in triple-negative breast cancer.
When Intensive Care Unit nurses delegate oral care to nursing assistants: A Qualitative Study
Authors: Li Xingru , Yao Lin , Yang Xinchen , Huang Meixia , Zhang Bo , Yu Tao , Tang Yun 
Published: October 2023

Abstract
Background
Although oral hygiene is closely related to various diseases, it is sub-optimal in the Intensive Care Unit (ICU). Oral care in the ICU is challenged by nursing workloads, low staffing, and higher acuity patients, there are few policies and written guidelines for oral care. Nurses often delegate oral care to nursing assistants (NAs) whose role is overlooked. This study is to explore the perspectives, obstacles, and challenges of NAs in the oral care of the ICU.
Methods
A qualitative study and semi-structured interviews were conducted with NAs in three ICU units, and Colaizzi’s phenomenological method was used to analyze the records.
Results
Initially, 13 NAs met the inclusion criteria, and two did not participate in this study as they refused to be recorded. Finally, 11 ICU NAs were interviewed, with three receiving face-to-face interviews and eight receiving telephone interviews. Using Colaizzi’s phenomenological method, two themes and eight subthemes emerged from the data, we examined the self-perception, barriers and challenges of NAs regarding oral care and identified the subthemes: (1) The target audience, frequency, and importance; (2) Role; (3) Evaluation; (4) Patient-related factors; (5) Oral care tools; (6) Psychology of NAs; (7) Lack of education and training; (8) Lack of team support.
Conclusion
Nursing assistants whose roles are overlooked by the nursing team are important members of the ICU team. Though oral care is closely related to disease prevention, it is rarely considered an essential task. Major barriers to implementing oral care in the ICU environment and patients include the psychological quality of participants, non-standard education and training, and inadequate team support. The expectation is that medical personnel will prioritize oral hygiene and recognize the significance of NAs in nursing work. Furthermore, future ICU oral care should investigate suitable tools and mouthwashes, simplified and standardized processes, standardized training, and multidisciplinary team collaboration.