bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative anatomy, histochemistry and phytochemistry of three species of the genus TarenayaRaf
Authors: dos Santos Magalhães Cledson , da Silva Flávia Carolina Lins , Randau Karina Perrelli 
Published: January 2024

AbstractThis study addresses the morphological similarities and taxonomic complexity of the genus Tarenaya Raf. of the family Cleomaceae Bercht. & J. Presl as well as the medicinal use of their species. The research compares potential anatomical diagnostic characters of the vegetative organs of species Tarenaya aculeata, Tarenaya diffusa, and Tarenaya spinosa to determine authenticity parameters. The study also carried out histochemical and phytochemical analyses of leaf blades to explore the medicinal use of these species. Semipermanent slides containing cross sections of the stems, petioles, and leaf blades, as well as paradermal sections of leaf blades, were prepared. The analyses were carried out in light and polarized optical microscopy. The histochemical analysis included different reagents depending on the target metabolite, which were analyzed by optical and fluorescence microscopy. Phytochemical tests of the methanolic extracts of the leaves were performed using thin layer chromatography. Anatomical characterization showed the characters of general occurrence in the family Cleomaceae and those that allow the differentiation of T. aculeata, T. diffusa, and T. spinosa. Histochemistry revealed the synthesis and/or storage sites of the metabolites and phytochemical tests; it was possible to observe the presence of different metabolites. The results bring comparative data on the anatomical and histochemical characterization of the species, thus increasing the taxonomic knowledge of the genus Tarenaya.Research Highlights
The comparative anatomy of three Tarenaya from Brazil was studied.
Anatomical differences in different vegetative organs differ species.
Differences in histochemistry and phytochemistry.
Drug‐related: A case of idiopathic mesenteric phlebosclerosis combined with calcification of the esophageal veins
Authors: Liu Chang , Li Li 
Published: January 2024

AbstractIdiopathic mesenteric phlebosclerosis (IMP) is characterized by mesenteric vein calcification and ischemic bowel disease. We describe a unique clinical case of IMP in a patient with a history of oral administration of various drugs, including traditional Chinese medicines (mainly selfheal), vitamin D, and calcium supplements. The disease was not diagnosed in its early stages and was later detected because of the initial symptoms of chest tightness and difficulties in swallowing. During medical examination, esophageal venous sinuses were found through gastroscopy, and CT revealed thickening and widespread calcification of the colonic wall (esophageal wall calcification). Moreover, typical purple‐brown changes in the colonic mucosa were found during colonoscopy. Microscopic examination showed more foam phagocyte, focal lymphocyte aggregation, small‐vessel proliferation, and surrounding collagen‐like deposition which is a typical finding of IMP. More specifically, the patient's mesenteric veins and colon veins were calcified, and the calcification extended to the esophageal veins. These findings were related to long‐term use of traditional Chinese medicines (mainly selfheal). It is possible that excessive intake vitamin D and calcium supplementation may have played a role in the occurrence of vascular calcification, which might have exacerbated the progression of IMP disease.
BRIX1 promotes ribosome synthesis and enhances glycolysis by selected translation of GLUT1 in colorectal cancer
Authors: Jiang Chunhui , Sun Longci , Wen Siyuan , Tian Yuan , Xu Chunjie , Xu Qing , Xue Hanbing 
Published: January 2024

AbstractBackgroundRibosome biogenesis protein BRX1 homolog (BRIX1) is critically required for the synthesis of the 60S ribosome subunit. However, the role and mechanism of BRIX1 in colorectal cancer (CRC) remain unclear.MethodsKyoto Encyclopedia of Gene and Genome pathway and Gene Ontology analyses were used for bioinformatics analysis. The rRNA levels were detected in CRC tissues and cells. Nascent RNA synthesis was detected via cellular immunofluorescence. The correlation was analyzed between patient Positron Emission Tomography‐Computed Tomography (PET–CT) values and their BRIX1 expression. The extracellular acidification rate (ECAR) and oxygen consumption rate were determined via live metabolic analyses. Polysome fractions were collected for BRIX1 mRNA used in translation. The orthotopic model and Cell Counting Kit‐8 (CCK8) assay were used to assess BRIX1 function in CRC.ResultsBRIX1 is a core protein involved in ribosome‐related pathway changes in CRC. Gene Ontology analysis showed that BRIX1 was primarily enriched in ribosome assembly and ribosome biogenesis pathways. In fresh CRC tissue, rRNA levels (5S, 5.8S, 18S and 28S) were higher in the BRIX1 high‐expression group than in the BRIX1 low‐expression group. Similarly, BRIX1 knockdown significantly decreased rRNA levels for 5S, 5.8S, 18S and 28S in CRC cells, whereas overexpression of BRIX1 significantly increased these levels. In addition, BRIX1 knockdown inhibited nascent RNA synthesis in CRC cells. In clinical data analysis, BRIX1 expression was related to the glucose uptake in PET–CT. BRIX1 knockdown significantly decreased the ECAR value, glucose uptake and lactic acid production in CRC cells, whereas BRIX1 overexpression significantly increased these. Furthermore, BRIX1 knockdown significantly decreased the protein expression of GLUT1, whereas BRIX1 overexpression significantly increased this; however, expression of BRIX1 mRNA was unaffected in either case. Blocking glycolysis by si‐GLUT1 or galactose reversed BRIX1 promotion of glycolysis and cell proliferation in CRC cells.
Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma
Authors: Su Ziwei , Wang Yang , Cao Jialing , Ma Jie , Wang Guangzhao , Ren Huijuan , Zhang Yihan , Sheng Kangliang , Zhu Xueying , Wang Yongzhong 
Published: January 2024

AbstractBackgroundThe primary reason for tumor‐related deaths worldwide is lung adenocarcinoma (LUAD). The oncogene IQ motif‐containing GTPase activating protein 3 (IQGAP3) is crucial for contributing to tumor initiation and progression. However, the precise function and molecular mechanism of IQGAP3 in LUAD remain unknown. The present study aimed to investigate the expression, prognosis, mechanism and tumor immunity associated with IQGAP3 in LUAD.MethodsThe relationship between IQGAP3 and the poor prognosis of LUAD was analyzed using The Cancer Genome Atlas (TCGA) database. This analysis was further validated on lung cancer tissues and cell lines. The function of IQGAP3 was investigated by silencing it in LUAD cell lines. To predict microRNA (miRNA) and long non‐coding RNA associated with IQGAP3, the starBase database was utilized, and the predictions were verified by enhancing the function of miRNA. Finally, the relationship between IQGAP3 and tumor immunity was evaluated using Spearman's correlation analysis.ResultsTCGA database revealed that higher levels of IQGAP3 were associated with advanced tumor stage, N stage and poor prognosis in LUAD patients. To confirm that, we conducted experiments on lung cancer tissues and cell lines and found that silencing IQGAP3 significantly inhibited tumor cell proliferation and migration. The expression of IQGAP3 showed a negative correlation with has‐miR‐101‐3p and has‐miR‐135a‐5p, whereas it showed a positive correlation with GSEC, AC005034.3 and TYMSOS. Furthermore, the introduction of miRNA‐mimics into lung cancer cell resulted in a significant inhibition of cancer cell growth and migration. Following that, the level of IQGAP3 showed a positive correlation with the infiltration of immune cells in tumors.ConclusionsThese results reveal that IQGAP3 significantly promotes LUAD progression and could serve as a prognostic biomarker for LUAD. Furthermore, IQGAP3 is most likely regulated by the GSEC/TYMSOS‐hsa‐miR‐101‐3p axis and the AC005034.3‐hsa‐miR‐135a‐5p axis in LUAD.
“We're all truly pulling in the exact same direction”: A qualitative study of attending and resident physician impressions of structured bedside interdisciplinary rounds
Authors: Mastalerz Katarzyna A. , Jordan Sarah R. , Townsley Nikki 
Published: January 2024

AbstractBackgroundPhysicians often hold leadership roles in implementing interdisciplinary rounds (IDR) in clinical settings, thus understanding physician perspectives of bedside IDR is crucial.ObjectiveTo understand physicians' perspectives of structured bedside IDR.DesignQualitative descriptive study.Setting and ParticipantsMedical and transitional year resident and hospitalist physicians participating in structured bedside IDR in a community teaching hospital affiliated with a large academic center.MethodsWe conducted semi‐structured interviews and focus groups with study participants about their experiences with participating in structured bedside IDR between July 2017 and April 2018. All interviews were recorded and professionally transcribed. We used thematic analysis to identify key themes.ResultsWe interviewed 41 resident physicians and 10 hospitalist physicians and discovered five key themes that highlight physician impressions of bedside IDR. Bedside IDR decreased unnecessary care, screened for risks/errors, created a shared mental model of care, and increased physician empathy for the patient and the interprofessional care team. Physicians felt patients valued the streamlined communication they experienced but questioned the intrusiveness of large healthcare teams. Challenges to bedside IDR included the meaningful engagement of key stakeholders and, particularly for resident physicians, the difficultly of managing unstandardized communication in a standardized time‐sensitive setting.ConclusionsStructured bedside IDR have the capacity to improve patient care, interprofessional teamwork, and physician empathy for patients and the interprofessional team. However, sustained leadership support and clearly defined rounds goals and objectives are necessary ingredients to maximize the benefits and address the challenges of bedside IDR.
Testing the accuracy of geographical profiling considering the effect of the number of crimes
Authors: Hanayama Aiko , Haginoya Shumpei , Kuraishi Hiroki 
Published: January 2024

AbstractThe present research compared geographic profiling (GP) methods, including spatial distribution strategies (SDS) and probability distance strategies (PDS), to account for the effect of the number of crimes on the accuracy of GP. Comparing the accuracy of GP methods among three burglar groups (a total of 333 offenders) with different sizes of crimes (1–5, 6–15 and 16 or more) committed before arrest showed that the accuracy decreased in a group with a larger size of crime. Comparing the accuracy of GP methods among the number of crimes (3–10) using 150 offenders who committed 10 or more crimes showed a slight improvement in the accuracy with the increasing number of crimes. In addition, the PDS (vs. SDS) showed a higher accuracy with the increasing number of crimes. The findings encourage practitioners to use the PDS and research implications are discussed.
Design and simulation of a germanium source dual‐metal dopingless tunnel FET as a label‐free biosensor
Authors: Dash Sidhartha , Panda Shwetapadma 
Published: January 2024

AbstractThis study presents a new dual‐metal dopingless tunnel field effect transistor with a Germanium source (GeS‐DM‐DLT) for label‐free biomolecule detection. Introducing a Ge source and dual‐metal gate provides improved drain current. We have considered an L‐shaped cavity at the top and bottom source metal region for investigating the sensitivity. The biosensor's sensitivity has been measured using the neutral biomolecules' dielectric constants (varying the k‐values in the cavity). The sensor's DC performance is investigated using transfer characteristics, BTBT rate, energy band, and electric field variation for different k‐values. The sensitivity performance of the proposed biosensor is evaluated in terms of different DC parameters (drain current, surface potential, subthreshold swing, interband tunneling rate, electric field) and RF parameters (parasitic capacitance, transconductance, cut‐off frequency, maximum frequency). The suggested biosensor offers a much‐improved SON of 9.86 × 108 and SRATIO of 1.94 × 104 for a dielectric constant of 22.0 at room temperature. Further research has been done to study the effects of dielectric materials, interface trap carriers (ITC), and temperature on drain current, drain current sensitivity, and other sensitivity parameters. The article also includes investigating the influence of the fill factor on sensitivity performance. The GeS‐DM‐DLT sensor performs best in fully‐filled conditions compared to the partially‐filled condition inside the cavity region.
Tree‐ring‐based reconstruction of the precipitation variability in the middle Taihang Mountains (1608–2021)
Authors: Hou Tiyuan , Zhao Xiaoen , Chen Feng , Chen Qiaomei , Wang Shijie , Hu Mao , Chen Youping , Zhang Heli , Cao Honghua 
Published: January 2024

AbstractUnderstanding historical droughts in northern China is challenging due to the limited time records of meteorological stations. In this study, we reconstructed the precipitation variability in the middle Taihang Mountains over 414 years by using the tree‐ring width of Pinus tabulaeformis. Based on our climate response analysis, Pinus tabulaeformis growth at the sampling sites was mainly restricted by the total precipitation from August of the previous year to June of the current year (P8‐C6), which was used as the dependent variable for precipitation reconstruction. Spatial analysis demonstrated that the reconstructed precipitation contained obvious spatial representativeness in the Taihang Mountains. We compared the precipitation sequence with other dryness/wetness sequences around the study area, revealing common drought events in most of northern China. Furthermore, the multi‐taper method (MTM) demonstrated a significant period of 2–4 years of the reconstructed precipitation. The precipitation variability in the Taihang Mountains may be influenced by the El Niño–Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO), and Atlantic Multidecadal Variability (AMV) and has exhibited a negative correlation in recent decades. This study has reconstructed the historical changes in wet and dry conditions to offer a reference for the utilization of modern water resources, especially by linking the decrease in precipitation in the study area with the downfall of the Ming Dynasty.
US federal government contracting for disaster management
Authors: Mena Carlos , Nair Anand 
Published: January 2024

AbstractDisasters affect hundreds of millions of people every year and the response of governments is crucial in alleviating the suffering of those affected. Despite the importance of contracting in response to disasters, research on this topic is conspicuous by its absence. This paper begins to address this gap by investigating the choice of procurement contract type by US federal agencies during disaster management operations. The research relies on 47,560 contracts issued by the US federal government in response to 14 major disasters between 2005 and 2016. We build on agency theory to investigate the choice of the contract type made by federal agencies at the different stages of a relief operation. This research provides empirical evidence of the key factors underpinning the choice of contract in the context of disaster management, namely the amount of spend per contract and the type of acquisition (product or service), and reveals the moderating role of the stage of the relief operation.
Opportunities, challenges and implications of medical tourism development in Hong Kong
Authors: Zhong Xiangqiong , Chan Chung‐Shing 
Published: January 2024

AbstractThis study revealed two different sets of push and pull factors affecting the medical travel decision process of two groups of mainland Chinese to Hong Kong, including potential and experienced medical tourists. The relations between identified factors and their willingness of personal visit and recommendation were also investigated. Regarding the recommendation intention, interpersonal acquisition and personal needs satisfaction are mostly considered by travelers in psychological level while recognized high medical reception capacity and quality are also prioritized in the pull aspect. However, local medical service constraints, perceived quality of medical experience shaped by healthcare suppliers, and destination attributes determine the two groups' responses to personal visit intention. Besides, the advantages of developing medical tourism in Hong Kong are implicated as can‐be‐perceived unique cultural attribute, mass tourism fame as medical tourism development basis, and reputation for high‐quality care formed with unintentional or deliberate promotion from third parties and various marketing channels.
Evidence of 13C‐lactate oxidation in the human brain from hyperpolarized 13C‐MRI
Authors: Uthayakumar Biranavan , Soliman Hany , Chen Albert P. , Bragagnolo Nadia , Cappelletto Nicole I.C. , Endre Ruby , Perks William J. , Ma Nathan , Heyn Chris , Keshari Kayvan R. , Cunningham Charles H. 
Published: January 2024

AbstractPurposeTo test the hypothesis that lactate oxidation contributes to the C‐bicarbonate signal observed in the awake human brain using hyperpolarized C MRI.MethodsHealthy human volunteers (N = 6) were scanned twice using hyperpolarized C‐MRI, with increased radiofrequency saturation of C‐lactate on one set of scans. C‐lactate, C‐bicarbonate, and C‐pyruvate signals for 132 brain regions across each set of scans were compared using a clustered Wilcoxon signed‐rank test.ResultsIncreased C‐lactate radiofrequency saturation resulted in a significantly lower C‐bicarbonate signal (p = 0.04). These changes were observed across the majority of brain regions.ConclusionRadiofrequency saturation of C‐lactate leads to a decrease in C‐bicarbonate signal, demonstrating that the C‐lactate generated from the injected C‐pyruvate is being converted back to C‐pyruvate and oxidized throughout the human brain.
Phantom evaluation of electrical conductivity mapping by MRI: Comparison to vector network analyzer measurements and spatial resolution assessment
Authors: He Zhongzheng , Lefebvre Pauline M. , Soullié Paul , Doguet Martin , Ambarki Khalid , Chen Bailiang , Odille Freddy 
Published: January 2024

AbstractPurposeTo evaluate the performance of various MR electrical properties tomography (MR‐EPT) methods at 3 T in terms of absolute quantification and spatial resolution limit for electrical conductivity.MethodsAbsolute quantification as well as spatial resolution performance were evaluated on homogeneous phantoms and a phantom with holes of different sizes, respectively. Ground‐truth conductivities were measured with an open‐ended coaxial probe connected to a vector network analyzer (VNA). Four widely used MR‐EPT reconstruction methods were investigated: phase‐based Helmholtz (PB), phase‐based convection‐reaction (PB‐cr), image‐based (IB), and generalized‐image‐based (GIB). These methods were compared using the same complex images from a 1 mm‐isotropic UTE sequence. Alternative transceive phase acquisition sequences were also compared in PB and PB‐cr.ResultsIn large homogeneous phantoms, all methods showed a strong correlation with ground truth conductivities (r > 0.99); however, GIB was the best in terms of accuracy, spatial uniformity, and robustness to boundary artifacts. In the resolution phantom, the normalized root‐mean‐squared error of all methods grew rapidly (>0.40) when the hole size was below 10 mm, with simplified methods (PB and IB), or below 5 mm, with generalized methods (PB‐cr and GIB).ConclusionVNA measurements are essential to assess the accuracy of MR‐EPT. In this study, all tested MR‐EPT methods correlated strongly with the VNA measurements. The UTE sequence is recommended for MR‐EPT, with the GIB method providing good accuracy for structures down to 5 mm. Structures below 5 mm may still be detected in the conductivity maps, but with significantly lower accuracy.
Anatomical location and number of injection sites of intravesical OnabotulinumtoxinA for females with refractory idiopathic overactive bladder: A scoping review
Authors: Kelsey Ellen , Wynn Jessica , Holmes Angela , McLeod Kathryn 
Published: January 2024

AbstractAimsThe negative impact on quality of life and the economic‐related burden to the patient and the health care system associated with idiopathic overactive bladder (iOAB) is well‐documented. Intradetrusor OnabotulinumtoxinA (BTN/A) injections are a well‐used treatment modality for the management of overactive detrusor refractory to medical management, with well‐documented efficacy and safety profiles. There is currently no best practice guideline for the administration of BTN/A for this procedure and historically the trigone of the bladder has been excluded from the injection paradigm given the risk of vesicoureteral reflux (VUR).MethodsA scoping review methodology was employed to assess available literature to evaluate current techniques used. There is emerging literature that the inclusion of the trigone may increase the efficacy of the procedure, while maintaining a similar adverse effect profile. Similar results could also be obtained by decreasing the number of injection sites. A scoping review was completed with systematic methodology using the Preferred Systematic Reviews and Meta Analyses extension for Scoping Review checklist. The search strategy looked to evaluate BTN/A and number of injection sites and the inclusion of the trigone in female patients with iOAB. Studies with male or neurogenic bladder only were excluded. Mixed studies were included. A specialist research librarian was engaged, with supervision from a functional urologist using a combination of MeSH and natural language terms. Two investigators independently reviewed the titles and abstracts.ResultsTwelve articles were included and were published between 2005 and 2021. There was no evidence of VUR in any of the results. All but one study reported similar if not improved efficacy of trigone‐inclusion. Lower number of injection sites had similar efficacy profiles to higher numbers of intradetrusor injections.ConclusionsFurther high‐quality randomized control trials of trigone inclusion and reduction of injection sites are required. It is hoped that with further exploration of intraoperative methods for BTN/A injections, the development of universally accepted guidelines may optimize management and experiences for patients with iOAB.