bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Situ Measurement of the Water Vapor 18O/16O Isotope Ratio for Atmospheric and Ecological Applications
Authors: Lee Xuhui , Sargent Steve , Smith Ronald , Tanner Bert 
Published: May 2005

Abstract
In this paper a system for in situ measurement of H216O/H218O in air based on tunable diode laser (TDL) absorption spectroscopy is described. Laboratory tests showed that its 60-min precision (one standard deviation) was 0.21‰ at a water vapor volume mixing ratio of 2.67 mmol mol−1 (dewpoint temperature −10.8°C at sea level) and improved to 0.09 at 15.3 mmol mol−1 (dewpoint temperature 13.4°C). The TDL measurement of the vapor generated by a dewpoint generator differed from the equilibrium prediction by −0.11 ± 0.43‰ (mean ± one standard deviation). Its measurement of the ambient water vapor differed from the cold-trap/mass spectrometer method by −0.36 ± 1.43‰. The larger noise of the latter comparison was caused primarily by the difficulty in extracting vapor from air without altering its isotope content. In a 1-week test in Logan, Utah, in August 2003, the isotope ratio of water vapor in ambient air was positively correlated with the water vapor mixing ratio and also responded to wetting events (rain and irrigation) in an expected manner.
This system has been in continuous operation in New Haven, Connecticut, since December 2003. It is suggested that such uninterrupted measurement may open a new window on the hydrologic cycle, particularly processes involving phase changes of water, and can increase the power of the isotope method in ecological applications.
Shattering during Sampling by OAPs and HVPS. Part I: Snow Particles
Authors: Korolev Alexei , Isaac George A. 
Published: May 2005

Abstract
The data on cloud particle sizes and concentrations collected with the help of aircraft imaging probes [optical array probes OAP-2DC, OAP-2DP, and the High Volume Precipitation Spectrometer (HVPS)] are widely used for cloud parameterization and validation of remote sensing. The goal of the present work is to study the effect of shattering of ice particles during sampling. The shattering of ice particles may occur due to 1) mechanical impact with the probe arms prior to their entering the sample volume, and 2) fragmentation due to interaction with turbulence and wind shear generated by the probe housing. The effect of shattering is characterized by the shattering efficiency that is equal to the ratio of counts of disintegrated particles, to all counts. The shattering efficiency depends on the habit, size, and density of ice particles, probe inlet design, and airspeed. For the case of aggregates, the shattering efficiency may reach 10% or even more. The shattering of ice particles results in an overcounting of small particles and an undercounting of large ones. The number of fragments in the images of shattered particles may reach several hundreds. It was found that particles as small as 600 μm may shatter after impact with the probe arms. The effect of particle shattering should be taken into account during data analysis and carefully considered in future designs of airborne cloud particle size spectrometers.
Multi-PRI Signal Processing for the Terminal Doppler Weather Radar. Part I: Clutter Filtering
Authors: Cho John Y. N. , Chornoboy Edward S. 
Published: May 2005

Abstract
Multiple pulse repetition interval (multi-PRI) transmission is part of an adaptive signal transmission and processing algorithm being developed to aggressively combat range–velocity ambiguity in weather radars. In the past, operational use of multi-PRI pulse trains has been hampered due to the difficulty in clutter filtering. This paper presents finite impulse response clutter filter designs for multi-PRI signals with excellent magnitude and phase responses. These filters provide strong suppression for use on low-elevation scans and yield low biases of velocity estimates so that accurate velocity dealiasing is possible. Specifically, the filters are designed for use in the Terminal Doppler Weather Radar (TDWR) and are shown to meet base data bias requirements equivalent to the Federal Aviation Administration’s specifications for the current TDWR clutter filters. Also an adaptive filter selection algorithm is proposed that bases its decision on clutter power estimated during an initial long-PRI surveillance scan. Simulations show that this adaptive algorithm yields satisfactory biases for reflectivity, velocity, and spectral width. Implementation of such a scheme would enable automatic elimination of anomalous propagation signals and constant adjustment to evolving ground clutter conditions, an improvement over the current TDWR clutter filtering system.
Microwave Rainfall Estimation over Coasts
Authors: McCollum Jeffrey R. , Ferraro Ralph R. 
Published: May 2005

AbstractThe microwave coastal rain identification procedure that has been used by NASA for over 10 yr, and also more recently by NOAA, for different instruments beginning with the Special Sensor Microwave Imager (SSM/I), is updated for use with Tropical Rainfall Measuring Mission (TRMM) Microwave Imager (TMI) and Advanced Microwave Scanning Radiometer (AMSR)-[Earth Observing System (EOS)] E microwave data. Since the development of the SSM/I algorithm, a wealth of both space-based and ground-based radar-rainfall estimates have become available, and here some of these data are used with collocated TMI and AMSR-E data to improve the estimation of coastal rain areas from microwave data. Two major improvements are made. The first involves finding the conditions where positive rain rates should be estimated rather than leaving the areas without estimates as in the previous algorithm. The second is a modification to the final step of the rain identification method; previously, a straight brightness temperature cutoff was used, but this is modified to a polarization-corrected temperature criterion. These modifications are made for the TRMM version 6 product release and the third (1 September) release of AMSR-E products to the public, both in 2004. The modifications are slightly different for each of these two sensors.
Some Concerns when Using Data from the Cooperative Weather Station Networks: A Nebraska Case Study
Authors: Wu Hong , Hubbard Kenneth G. , You Jinsheng 
Published: May 2005

Abstract
In this study, daily temperature and precipitation amounts that are observed by the Cooperative Observer Program (COOP) were compared among geographically close stations. Hourly observations from nearby Automatic Weather Data Network (AWDN) stations were utilized to resolve the discrepancies between the observations during the same period. The statistics of maximum differences in temperature and precipitation between COOP stations were summarized. In addition, the quantitative measures of the deviations between COOP and AWDN stations were expressed by root-mean-square error, mean absolute error, and an index of agreement. The results indicated that significant discrepancies exist among the daily observations between some paired stations because of varying observation times, observation error, sensor error, and differences in microclimate exposure. The purpose of this note is to bring attention to the problem and offer guidance on the use of daily observations in the comparison and creation of weather maps. In addition, this study demonstrates approaches for identifying the sources of the discrepancies in daily temperature and precipitation observations. The findings will be useful in the quality assurance (QA) procedures of climate data.
Off-axis Flexure Test: A New Method for Obtaining In-plane Shear Properties
Authors: Mujika F. , Valea A. , Gañán P. , Mondragon I. 
Published: May 2005

Off-axis three-point flexure test for unidirectional composites is proposed as a new method for obtaining in-plane shear modulus and in-plane shear strength. The method of determination of the in-plane shear modulus GLT has been explained in a previous work where the displacement field of an off-axis flexure test was analyzed. In the present work, besides summarizing the calculation equations of GLT, the condition of small displacements is analyzed. Otherwise, an error analysis is carried out in order to study the influence of the calculated value of GLT on the other elastic parameters. Normal and shear stresses in the fiber–matrix interface change from point to point in the specimen depending on the fiber orientation angle and specimen geometry. A critical point of failure that depends on the fiber orientation angle is determined and optimum conditions for obtaining in-plane shear strength are discussed. Experiments for different fiber orientations and geometric conditions have been carried out for IM7/8552 epoxy matrix based carbon fiber reinforced unidirectional composite material.
Splenic marginal zone lymphoma: proposal of new diagnostic and prognostic markers identified after tissue and cDNA microarray analysis
Authors: Ruiz-Ballesteros Elena , Mollejo Manuela , Rodriguez Antonia , Camacho Francisca I. , Algara Patrocinio , Martinez Nerea , Pollán Marina , Sanchez-Aguilera Abel , Menarguez Javier , Campo Elias , Martinez Pedro , Mateo Marisol , Piris Miguel A. 
Published: May 2005

Abstract
Splenic marginal zone lymphoma (SMZL) is a newly recognized lymphoma type whose precise molecular pathogenesis is still essentially unknown. This hampers differential diagnosis with other small B-cell malignancies. With the aim of characterizing this tumor more comprehensively, and of identifying new diagnostic and prognostic markers, we performed cDNA microarray expression profiling and tissue microarray (TMA) immunohistochemical studies in a relatively large series of 44 SMZLs. The results were related to immunoglobulin heavy chain variable region (IgVH) mutational status and clinical outcome. SMZLs display a largely homogenous signature, implying the existence of a single molecular entity. Of the genes deregulated in SMZLs, special mention may be made of the genes involved in B-cell receptor (BCR) signaling, tumor necrosis factor (TNF) signaling and nuclear factor-κB (NF-κB) activation, such as SYK, BTK, BIRC3, TRAF3, and LTB. Other genes observed were SELL and LPXN, which were highly expressed in spleen, and lymphoma oncogenes, such as ARHH and TCL1. In contrast, the genes CAV1, CAV2, and GNG11 located in 7q31, a commonly deleted area, were down-regulated in the entire series. A comparison with the genes comprising the signature of other small B-cell lymphomas identified 3 genes whose expression distinguishes SMZL, namely ILF1, SENATAXIN, and CD40. Shorter survival was associated with CD38 expression, naive IgVH genes, and the expression of a set of NF-κB pathway genes, including TRAF5, REL, and PKCA. (Blood. 2005;106:1831-1838)
Inhibitors of XIAP sensitize CD40-activated chronic lymphocytic leukemia cells to CD95-mediated apoptosis
Authors: Kater Arnon P. , Dicker Frank , Mangiola Massimo , Welsh Kate , Houghten Richard , Ostresh John , Nefzi Adel , Reed John C. , Pinilla Clemencia , Kipps Thomas J. 
Published: May 2005

Abstract
Patients with chronic lymphocytic leukemia (CLL) treated with adenovirus CD154 (Ad-CD154, CD40 ligand [CD40L]) gene therapy experienced rapid reductions in leukemia cell counts and lymph node size associated with the induced expression of Fas (CD95). However, CLL cells initially resist CD95-mediated apoptosis within the first 3 days after CD40 ligation in vitro. Thereafter, they become sensitive, which is associated with the CD40-induced expression of the proapoptotic protein B-cell leukemia 2 homology 3 (BH3) interacting domain death agonist (Bid). We hypothesized that the initial resistance to CD95-mediated apoptosis may be due to the high-level expression of X-linked inhibitor of apoptosis protein (XIAP) by CLL cells. Consistent with this, CLL cells from patients 1 day after treatment with autologous Ad-CD154-transduced CLL cells became sensitive to CD95-mediated apoptosis following treatment with a novel XIAP inhibitor, 1540-14. Similarly, 1540-14 specifically enhanced CD95-mediated apoptosis of CLL cells following CD40 ligation in vitro. Immunoblot analyses demonstrated that treatment with 1540-14 allowed CD40-stimulated CLL cells to experience high-level activation of caspases-8 and -3 and cleavage of poly(adenosine diphosphate [ADP]-ribose) polymerase following CD95 ligation. This study demonstrates that distal apoptosis regulators contribute to the initial resistance of CD40-activated CLL cells to CD95-mediated apoptosis and suggests that XIAP inhibitors might enhance the effectiveness of immune-based treatment strategies that target CD40, such as CD154 gene therapy. (Blood. 2005;106:1742-1748)
Effect of transcription-factor concentrations on leukemic stem cells
Authors: Rosenbauer Frank , Koschmieder Steffen , Steidl Ulrich , Tenen Daniel G. 
Published: May 2005

AbstractIncreasing evidence suggests that leukemias are sustained by leukemic stem cells. However, the molecular pathways underlying the transformation of normal cells into leukemic stem cells are still poorly understood. The involvement of a small group of key transcription factors into this process was suggested by their frequent mutation or down-regulation in patients with acute myeloid leukemia (AML). Recent findings in mice with hypomorphic transcription-factor genes demonstrated that leukemic stem-cell formation in AML could directly be caused by reduced transcription-factor activity beyond a critical threshold. Most interestingly, those experimental models and the paucity of biallelic null mutations or deletions in transcription-factor genes in patients suggest that AML is generally associated with graded down-regulation rather than complete disruption of transcription factors. Here, we discuss the effects of transcription-factor concentrations on hematopoiesis and leukemia, with a focus on the regulation of transcription-factor gene expression as a major mechanism that alters critical threshold levels during blood development and cancer.
Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation
Authors: Iwasaki Hiromi , Somoza Chamorro , Shigematsu Hirokazu , Duprez Estelle A. , Iwasaki-Arai Junko , Mizuno Shin-ichi , Arinobu Yojiro , Geary Kristin , Zhang Pu , Dayaram Tajhal , Fenyus Maris L. , Elf Shannon , Chan Susan , Kastner Philippe , Huettner Claudia S. , Murray Richard , Tenen Daniel G. , Akashi Koichi 
Published: May 2005

AbstractThe PU.1 transcription factor is a key regulator of hematopoietic development, but its role at each hematopoietic stage remains unclear. In particular, the expression of PU.1 in hematopoietic stem cells (HSCs) could simply represent “priming” of genes related to downstream myelolymphoid lineages. By using a conditional PU.1 knock-out model, we here show that HSCs express PU.1, and its constitutive expression is necessary for maintenance of the HSC pool in the bone marrow. Bone marrow HSCs disrupted with PU.1 in situ could not maintain hematopoiesis and were outcompeted by normal HSCs. PU.1-deficient HSCs also failed to generate the earliest myeloid and lymphoid progenitors. PU.1 disruption in granulocyte/monocyte-committed progenitors blocked their maturation but not proliferation, resulting in myeloblast colony formation. PU.1 disruption in common lymphoid progenitors, however, did not prevent their B-cell maturation. In vivo disruption of PU.1 in mature B cells by the CD19-Cre locus did not affect B-cell maturation, and PU.1-deficient mature B cells displayed normal proliferation in response to mitogenic signals including the cross-linking of surface immunoglobulin M (IgM). Thus, PU.1 plays indispensable and distinct roles in hematopoietic development through supporting HSC self-renewal as well as commitment and maturation of myeloid and lymphoid lineages.
Negative control of basophil expansion by IRF-2 critical for the regulation of Th1/Th2 balance
Authors: Hida Shigeaki , Tadachi Masumi , Saito Takashi , Taki Shinsuke 
Published: May 2005

AbstractAlthough basophils are known to produce interleukin 4 (IL-4), the roles of these cells have been documented only in mice infected with parasites or in the effector phase of allergic inflammations. Here we show that naive mice lacking the transcription factor, interferon regulatory factor 2 (IRF-2), exhibited signal transducer and activator of transcription 6 (Stat6)–independent expansion of basophils in the periphery. IRF-2 appeared to act autonomously in the cells to negatively regulate the expansion of, but not cytokine production by, basophils. Spontaneous Th2 polarization of CD4+ T cells was observed in these mice and the genetic reduction of basophil numbers by mutating the Kit gene abolished such a polarization in vivo. We also found that both basophils and IL-4 derived from them were indeed essential for Th2 development under neutral conditions in vitro. Furthermore, neutralization of IL-3 abolished IL-4 production by basophils during Th1/Th2 differentiation cultures and subsequent Th2 development. These results indicated that basophils acted as a cellular converter to turn the neutral IL-3 into the Th2-inducing IL-4 during the initiation of Th1/Th2 differentiation. Thus, the negative regulatory role of IRF-2 on the basophil population size is critically important for preventing excess Th2 polarization and the Th1/Th2 balance in naive animals.
Comparative genomics of Helicobacter pylori isolates recovered from ulcer disease patients in England
Authors: Kauser Farhana , Hussain M Abid , Ahmed Irshad , Srinivas Sriramula , Devi S Manjulata , Majeed Ahmed A , Rao K Rajender , Khan Aleem A , Sechi Leonardo A , Ahmed Niyaz 
Published: May 2005

Abstract

Background
Genomic diversity of H. pylori from many different human populations is largely unknown. We compared genomes of 65 H. pylori strains from Nottingham, England. Molecular analysis was carried out to identify rearrangements within and outside the cag-pathogenicity-island (cag PAI) and DNA sequence divergence in candidate genes. Phylogenetic analysis was carried out based on various high-resolution genotyping techniques.

Results
Analyses of virulence genes (cag T, cag E, cag A, vac A, ice A, oip A and bab B) revealed that H. pylori strains from England are genetically distinct from strains obtained from other countries. The toxigenic vac A s1m1 genotype was found to be less common and the plasticity region cluster was found to be disrupted in all the isolates. English isolates showed a predominance of ice A1 alleles and a functional proinflammatory oip A gene. The English H. pylori gene pool revealed several Asian/oriental features. This included the predominance of cag A – glr (cag A right junction) motif types III and II (up to 42%), presence of vac A m1c alleles and phylogenetic affinity towards East Asian / Amerindian gene pools based on fluorescent amplified fragment length polymorphism (FAFLP) analysis and glm M sequence analysis.

Conclusion
Overall, our results demonstrated genetic affinities of H. pylori in England with both European and the Asian gene pools and some distinctive genetic features of virulence genes that may have evolved in this important European population.
Surface TRAIL decoy receptor-4 expression is correlated with TRAIL resistance in MCF7 breast cancer cells
Authors: Sanlioglu Ahter D , Dirice Ercument , Aydin Cigdem , Erin Nuray , Koksoy Sadi , Sanlioglu Salih 
Published: May 2005

AbstractBackgroundTumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. Despite this promising feature, TRAIL resistance observed in cancer cells seriously challenged the use of TRAIL as a death ligand in gene therapy. The current dispute concerns whether or not TRAIL receptor expression pattern is the primary determinant of TRAIL sensitivity in cancer cells. This study investigates TRAIL receptor expression pattern and its connection to TRAIL resistance in breast cancer cells. In addition, a DcR2 siRNA approach and a complementary gene therapy modality involving IKK inhibition (AdIKKβKA) were also tested to verify if these approaches could sensitize MCF7 breast cancer cells to adenovirus delivery of TRAIL (Ad5hTRAIL).MethodsTRAIL sensitivity assays were conducted using Molecular Probe's Live/Dead Cellular Viability/Cytotoxicity Kit following the infection of breast cancer cells with Ad5hTRAIL. The molecular mechanism of TRAIL induced cell death under the setting of IKK inhibition was revealed by Annexin V binding. Novel quantitative Real Time RT-PCR and flow cytometry analysis were performed to disclose TRAIL receptor composition in breast cancer cells.ResultsMCF7 but not MDA-MB-231 breast cancer cells displayed strong resistance to adenovirus delivery of TRAIL. Only the combinatorial use of Ad5hTRAIL and AdIKKβKA infection sensitized MCF7 breast cancer cells to TRAIL induced cell death. Moreover, novel quantitative Real Time RT-PCR assays suggested that while the level of TRAIL Decoy Receptor-4 (TRAIL-R4) expression was the highest in MCF7 cells, it was the lowest TRAIL receptor expressed in MDA-MB-231 cells. In addition, conventional flow cytometry analysis demonstrated that TRAIL resistant MCF7 cells exhibited substantial levels of TRAIL-R4 expression but not TRAIL decoy receptor-3 (TRAIL-R3) on surface. On the contrary, TRAIL sensitive MDA-MB-231 cells displayed very low levels of surface TRAIL-R4 expression. Furthermore, a DcR2 siRNA approach lowered TRAIL-R4 expression on surface and this sensitized MCF7 cells to TRAIL.ConclusionThe expression of TRAIL-R4 decoy receptor appeared to be well correlated with TRAIL resistance encountered in breast cancer cells. Both adenovirus mediated IKKβKA expression and a DcR2 siRNA approach sensitized MCF7 breast cancer cells to TRAIL.
Behaviour sequelae following acute Kawasaki disease
Authors: Carlton-Conway Daniel , Ahluwalia Raju , Henry Lucy , Michie Colin , Wood Louise , Tulloh Robert 
Published: May 2005

Abstract
Background
Kawasaki disease is a systemic vasculitis and may affect cerebral function acutely. The aim of the present study was to measure a number of behaviour and social parameters within a cohort of Kawasaki disease patients.

Methods
Parents of children with past diagnosis of Kawasaki disease were recruited to complete several behaviour screening questionnaires. Sixty five sets of questionnaires relating to the patient cohort received were eligible for inclusion. Two control groups were used, a hospital (HC) control and a sibling control (SC) group.

Results
40% of the Kawasaki disease group showed elevated internalising scores in the clinical or borderline-clinical range. This compared with 18% of hospital controls and 13% of sibling controls. Additionally, the Kawasaki disease (KD) group were shown to be experiencing greater overall total difficulties when compared with the controls (KD 13.7, HC 8.6, SC 8.9). The KD group attained higher behavioural scores within the internalising sub-categories of somatic problems (KD 61, HC 57, SC 54) and withdrawn traits (KD 56, HC 53, SC 51). The KD group were also shown to be suffering more thought problems (KD 57, HC 53, SC 50) compared with the controls. Further difficulties relating to conduct (KD 3.3, HC 1.4) and social interactions (KD 6.7, HC 8.3) are also highlighted for the KD group compared with hospital controls. Positron emission tomograms were performed on nine patients to investigate severe behavioural problems. Three showed minor changes, possibly a resolving cerebral vasculopathy.

Conclusion
Kawasaki disease can be associated with significant behavioural sequelae. This is an important consideration in the long-term follow up and referral to a clinical psychologist may be necessary in selected patients.