bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro evaluation of cell viability and expression profile of growth factors in mouse Sertoli cells exposed to Delta-9-tetrahydrocannabinol: a mechanistic insight into the cannabinoid-induced testicular toxicity
Authors: Mohammadpour-Asl Shadi , Roshan-Milani Shiva , Abdollahzade Fard Amin , Golchin Ali 
Published: November 2023

AbstractThe potentially adverse effects of cannabis (marijuana), a common leisure compound, on male reproductive performance are a reason for concern. δ-9-tetrahydrocannabinol (THC), the primary active component of marijuana alters testicular cells’ proliferation and function which affects male fertility and causes testicular cells dysfunction and apoptosis. The main objective of this study was to investigate the possible mechanism underlying the toxic effects of THC with a mechanistic insight into Sertoli cell-based reproductive dysfunction. The Mus musculus Sertoli cell line (TM4) was cultured and exposed to different concentrations of THC and, MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was then performed for evaluating cell viability. The expression of caspase-3 gene and genes related to growth factors were analyzed by real-time RT-PCR. Western blotting was performed for evaluating protein expression level. THC concentration-dependently decreased the TM4 viability with a significant effect starting at concentration of 1 μM and reaching about 75% of the control level at the concentration of 50 μM (IC25). Moreover, caspase-3 mRNA expression levels significantly increased while growth factors mRNA levels decreased in THC-exposed cells compared to unexposed cells. There was also a significant reduction in related protein levels in THC group. Administration of the THC promotes cytotoxic and apoptotic effects on TM4 cells partly through down-regulation of growth factors expression. Increased apoptosis, over expression of caspase-3, and down-regulation of growth factors expression in Sertoli cells exposed to THC may be a reflection of THC-induced testicular toxicity, which may be partly involved in infertility associated with marijuana smoking or medical cannabis use.
Lacosamide dosing in patients receiving continuous renal replacement therapy
Authors: Chaijamorn Weerachai , Phunpon Sathian , Sathienluckana Thanompong , Charoensareerat Taniya , Pattharachayakul Sutthiporn , Rungkitwattanakul Dhakrit , Srisawat Nattachai 
Published: November 2023

Abstract
Background
Lacosamide is one of the anticonvulsants used in critically ill patients. This study aimed to suggest appropriate lacosamide dosing regimens in critically ill patients receiving continuous renal replacement therapy (CRRT) via Monte Carlo simulations.

Methods
Mathematical models were created using published demographic and pharmacokinetics in adult critically ill patients. CRRT modalities with different effluent rates were added into the models. Lacosamide regimens were evaluated on the probability of target attainment (PTA) using pharmacodynamic targets of trough concentrations and area under the curve within a range of 5–10 mg/L and 80.25–143 and 143–231 mg*h/L for the initial 72 h-therapy, respectively. Optimal regimens were defined from regimens that yielded the highest PTA. Each dosing regimen was tested in a group of different 10,000 virtual patients.

Results
Our results revealed the optimal lacosamide dosing regimen of 300–450 mg/day is recommended for adult patients receiving both CRRT modalities with 20–25 effluent rates. The dose of 600 mg/day was suggested in higher effluent rate of 35 mL/kg/h. Moreover, a patient with body weight > 100 kg was less likely to attain the targets.

Conclusions
Volume of distribution, total clearance, CRRT clearance and body weight were significantly contributed to lacosamide dosing. Clinical validation of the finding is strongly indicated.
Insights into the underlying pathogenesis and therapeutic potential of endoplasmic reticulum stress in degenerative musculoskeletal diseases
Authors: Wen Ze-Qin , Lin Jun , Xie Wen-Qing , Shan Yun-Han , Zhen Ge-Hua , Li Yu-Sheng 
Published: November 2023

AbstractDegenerative musculoskeletal diseases are structural and functional failures of the musculoskeletal system, including osteoarthritis, osteoporosis, intervertebral disc degeneration (IVDD), and sarcopenia. As the global population ages, degenerative musculoskeletal diseases are becoming more prevalent. However, the pathogenesis of degenerative musculoskeletal diseases is not fully understood. Previous studies have revealed that endoplasmic reticulum (ER) stress is a stress response that occurs when impairment of the protein folding capacity of the ER leads to the accumulation of misfolded or unfolded proteins in the ER, contributing to degenerative musculoskeletal diseases. By affecting cartilage degeneration, synovitis, meniscal lesion, subchondral bone remodeling of osteoarthritis, bone remodeling and angiogenesis of osteoporosis, nucleus pulposus degeneration, annulus fibrosus rupture, cartilaginous endplate degeneration of IVDD, and sarcopenia, ER stress is involved in the pathogenesis of degenerative musculoskeletal diseases. Preclinical studies have found that regulation of ER stress can delay the progression of multiple degenerative musculoskeletal diseases. These pilot studies provide foundations for further evaluation of the feasibility, efficacy, and safety of ER stress modulators in the treatment of musculoskeletal degenerative diseases in clinical trials. In this review, we have integrated up-to-date research findings of ER stress into the pathogenesis of degenerative musculoskeletal diseases. In a future perspective, we have also discussed possible directions of ER stress in the investigation of degenerative musculoskeletal disease, potential therapeutic strategies for degenerative musculoskeletal diseases using ER stress modulators, as well as underlying challenges and obstacles in bench-to-beside research.
Dietary counselling plus omega-3 supplementation in the treatment of generalized anxiety disorder: protocol for a randomized wait-list controlled pilot trial (the “EASe-GAD Trial”)
Authors: Aucoin Monique , LaChance Laura , van der Wurff Inge , Miller Sean , Naidoo Umadevi , Jenkins Andrew , Cooley Kieran 
Published: November 2023

Abstract
Background
Anxiety disorders are prevalent and disabling conditions involving excessive worry and tension. Generalized anxiety disorder (GAD), the most common anxiety disorder, affects 5% of individuals from high-income countries and many individuals report that treatment options are not accessible, effective, or tolerable. Clinical evidence suggests that nutrition interventions, based on the Mediterranean diet and supplementation of omega-3 fatty acids, can significantly improve symptoms of depression; however, the effect of nutrition interventions on anxiety symptoms has not been studied in a clinical population. The primary objective of the present study is to assess the feasibility and acceptability of a dietary counselling and omega-3 fatty acid supplementation intervention delivered to adult women with GAD. The secondary objectives include assessing changes in anxiety symptom severity, assessing changes in quality of life, assessing changes in biomarkers, and evaluating the components of the program.

Methods
This study is a randomized, wait-list controlled pilot trial delivering a 12-week, dietary counselling intervention and omega-3 supplementation to 50 adult women with GAD. Participants will complete seven individual counselling sessions which include education, personalized recommendations, mindful eating techniques, motivational interviewing, and goal setting. They will be provided with recipes, instructional videos, and food items. The intervention is designed based on the Social Cognitive Theory and previous research that has been done by the author team to identify dietary constituents with the most evidence to support their use in the treatment of anxiety disorders. Questionnaires and blood work will be completed at baseline, after the waiting period (for those in the waitlist group), and after the intervention.

Discussion
Results from this study will lay the foundation for future large-scale studies in this area and may provide preliminary evidence of the role of diet counselling and omega-3 supplementation in the management of GAD. Research on the role of nutrition in psychiatric care has been identified as a priority by a number of international organizations. The present trial directly addresses the call for the research that is most needed to advance the field.

Trial registration
This protocol was registered at ClinicalTrials.gov on October 10, 2022; NCT05573672.
Trial sponsor: The Canadian College of Naturopathic Medicine, 1255 Sheppard Ave E, Toronto, ON M2K 1E2, 416–498-1255.
Steering committee: Composed of MA, LL, KC, IvdW, SM, UN, AJ. The committee meets monthly to oversee the trial.
Protocol identifier: CCNM_EASe-GADCT_2201v4.
Not quite eye to A.I.: student and teacher perspectives on the use of generative artificial intelligence in the writing process
Authors: Barrett Alex , Pack Austin 
Published: November 2023

AbstractGenerative artificial intelligence (GenAI) can be used to author academic texts at a similar level to what humans are capable of, causing concern about its misuse in education. Addressing the role of GenAI in teaching and learning has become an urgent task. This study reports the results of a survey comparing educators’ (n = 68) and university students’ (n = 158) perceptions on the appropriate use of GenAI in the writing process. The survey included representations of user prompts and output from ChatGPT, a GenAI chatbot, for each of six tasks of the writing process (brainstorming, outlining, writing, revising, feedback, and evaluating). Survey respondents were asked to differentiate between various uses of GenAI for these tasks, which were divided between student and teacher use. Results indicate minor disagreement between students and teachers on acceptable use of GenAI tools in the writing process, as well as classroom and institutional-level lack of preparedness for GenAI. These results imply the need for explicit guidelines and teacher professional development on the use of GenAI in educational contexts. This study can contribute to evidence-based guidelines on the integration of GenAI in teaching and learning.
The holistic effects of medical cannabis compared to opioids on pain experience in Finnish patients with chronic pain
Authors: Jylkkä Jussi , Hupli Aleksi , Nikolaeva Aleksandra , Alanen Sandra , Back Anna Erika , Lindqvist Sara , Krabbe Andreas , Lavie-Ajayi Maya , Kantonen Oskari 
Published: November 2023

Abstract
Background
Medical cannabis (MC) is increasingly used for chronic pain, but it is unclear how it aids in pain management. Previous literature suggests that MC could holistically alter the pain experience instead of only targeting pain intensity. However, this hypothesis has not been previously systematically tested.

Method
A retrospective internet survey was used in a sample of Finnish chronic pain patients (40 MC users and 161 opioid users). The patients evaluated statements describing positive and negative phenomenological effects of the medicine. The two groups were propensity score matched to control for possible confounding factors.

Results
Exploratory factor analysis revealed three experience factors: Negative Side Effects, Positive Holistic Effects, and Positive Emotional Effects. The MC group (matched n = 39) received higher scores than the opioid group (matched n = 39) in Positive Emotional Effects with large effect size (Rank-Biserial Correlation RBC = .71, p < .001), and in Holistic Positive Effects with medium effect size (RBC = .47, p < .001), with no difference in Negative Side Effects (p = .13). MC and opioids were perceived as equally efficacious in reducing pain intensity. Ratings of individual statements were exploratively examined in a post hoc analysis.

Conclusion
MC and opioids were perceived to be equally efficacious in reducing pain intensity, but MC additionally positively affected broader pain-related factors such as emotion, functionality, and overall sense of wellbeing. This supports the hypothesis that MC alleviates pain through holistically altering the pain experience.
Numerical modelling of traffic-induced dynamic loading on a two-story residential building
Authors: Shiferaw Henok Marie , Teshager Girma Moges , Hailu Solomon Aynalem , Shiferaw Tadiyos Marie 
Published: November 2023

Abstract
Background
This paper presents a numerical modelling procedure of dynamic loading due to traffic on a two-story residential building. In developing countries cities and towns, poor quality narrow road sections with high traffic density are common. In such cases, ground vibration due to traffic could be higher and lightweight buildings located closer are exposed to traffic-induced dynamic loading. Design codes require a proper assessment of such vibrations. However, a clear and definite procedure of assessment is not usually provided. This research presents an assessment procedure of dynamic loading due to traffic on a soil foundation system of light weight buildings based on numerical modeling. Traffic induced ground vibration acceleration amplitudes, frequencies and durations were measured, and the dynamic loads were calculated from measured vibration accelerations and vibrating mass of the vehicle. A two-story residential building with flexible square shallow footings was modelled together with the foundation soil using PLAXIS-2D. The dynamic load was modelled as harmonic loading considering the highest amplitude of vibration measured.

Results
On the calculation stage, static loading analysis, dynamic loading analysis and free vibration dynamic analysis were carried out. A maximum increase in extreme total displacement of the soil to 22.03 mm was observed after the dynamic loading from 18.96 mm extreme total displacement due to static loading. Extreme effective mean stress in the soil increased to 112.81 kPa from 110.5 kPa, due to the dynamic loading. In addition, a differential settlement of 3.14 mm between two adjacent footings was observed after the traffic induced ground vibration. Furthermore, the Mohr–Coulomb plastic points were observed to be concentrated to the side of the soil-foundation system which the dynamic load was acting on.

Conclusion
The regular exposure to traffic-induced vibrations may cause frequent change in stress and deformation response of the foundation-soil system. In areas where lightweight buildings are exposed to regular traffic induced vibrations, proper assessment of the effect should be carried out and measures should be taken to mitigate the problem. Improving road surfaces and limiting vehicle speed are possible remedial measures to reduce ground vibrations due to traffic.
Does financial repression inhibit or facilitate private investment? The case of Ethiopia
Authors: Fisseha Fentaw Leykun 
Published: November 2023

Abstract
This study examines the impact of financial repression (FR) on private investment in Ethiopia over the period 1980 to 2020. Investigating the consequences of FR policies makes use of the cointegration technique. The dynamic ordinary least square (DOLS) estimation result demonstrated that FR has a detrimental and statistically significant impact on private investment, resulting in considerable lost opportunities for private investors by driving away banks' productive investment. In addition, the structural reforms implemented since 2011 have a beneficial and significant influence on private investment. The effects of financial development, per capita GDP, and domestic lending to the private sector all produce similar consequences. Additionally, Ethiopia's private investment is negatively impacted by trade liberalization and inflation. The primary findings are used to infer potential policy implications.
Implementation of Precision Oncology in the National Healthcare System: A Statement Proposal Endorsed by Italian Scientific Societies
Authors: Fasola Gianpiero , Barducci Maria C. , Tozzi Valeria D. , Cavanna Luigi , Cinieri Saverio , Perrone Francesco , Pinto Carmine , Russo Antonio , Sapino Anna , Grossi Francesco , Aprile Giuseppe 
Published: November 2023

PURPOSEPrecision oncology (PO) promises positive results for patients. To date, in Italy, the effort to implement PO has been made autonomously by regional health institutions in a top-down fashion. This approach is not very efficient and jeopardizes patients' equal access to PO. Similar outcomes have been recorded in other Western countries. We tested a method of collaboration among professionals, scientific societies, and government institutions to facilitate the delivery of PO innovation to patients' bedsides.METHODWe designed an organizational research project on the basis of a bottom-up approach. We started by observing PO-related activities in five health care authorities (HCAs) in one Italian region. We then compared the issues that emerged with those of three additional HCAs in other Italian regions. Using the results of the initial observation and adopting validated multiple-step consensus methods, we finally derived 14 statements that were approved by the four main scientific societies of oncology and pathology at the national level.RESULTSThe 14 statements addressed the main issues linked to the implementation of PO in clinical practice. The strong professional consensus advocated for prompt adoption within the national healthcare system.CONCLUSIONSThe consensus on the statements that were obtained shows the importance of a synergistic effort among professionals, scientific societies, and health care institutions in defining homogeneous solutions for innovation implementation within the health care system.
Zeeman dependence of the quasiparticle scattering rate and ARPES in copper oxides and related materials
Authors: Kastrinakis George 
Published: November 2023

Abstract
Within a strongly interacting Fermi liquid framework, we calculate the effects of the Zeeman energy ω

H
for a finite magnetic field, in a metallic system with a van Hove peak in the density of states, located close to and below the Fermi surface. We find that the chemical potential increases with the square of ω

H
. We obtain a characteristic quasiparticle scattering rate linear in the maximum of ω

H
and temperature, both in the normal and the d-wave superconducting state. We predict that ARPES experiments in copper oxides, and related compounds, should be able to elucidate this behavior of the scattering rate, and in particular, the difference between spin up and down electrons.
Two Cases of Surreptitious Steroid Use Uncovered Utilizing Urine Synthetic Glucocorticoid Testing
Authors: Pape Bryn J , Kern Philip A 
Published: November 2023

Abstract
Although most patients are transparent regarding steroid use, rare patients use steroids surreptitiously, which can occasionally result in factitious Cushing syndrome or extensive diagnostic testing. We present 2 cases, 1 with factitious Cushing syndrome and the second with surreptitious steroid use resulting in abnormal laboratory results and a complicated clinical picture. Synthetic glucocorticoid urine testing was positive for triamcinolone acetonide and fluticasone propionate in case 1 and triamcinolone acetonide only in case 2, which clarified the diagnosis and minimized additional and potentially invasive testing.
Factors affecting poverty using a geographically weighted regression approach (case study of Java Island, 2020)
Authors: Belantika Bernica Tiyas , Rohmad Bagus , Arandita Hawa Dwi Nur , Hutasoit David R. , Kartiasih Fitri 
Published: November 2023

Poverty is still the main problem in development both at the national and regional levels. The poverty reduction program carried out has not paid attention to spatial aspects so that the policies taken are often not on target. This study aims to see the spatial pattern of poverty in Java Island which includes Banten, DKI Jakarta, West Java, Central Java, DI Yogyakarta and East Java. The method used is geographically weighted regression (GWR) with addiptive weighting of the Gaussian Kernel which is processed with QGIS, Geoda and GWR4 software. This approach can identify spatial patterns that cannot be identified in ordinary regression analysis as found in previous studies. The data used in this study is secondary data in 2020 sourced from the Badan Pusat Statistik (BPS) and government website. The results of the study showed positive and group spatial autocorrelation in 34 districts/cities. There are 65 districts/cities in Java Island only affected by HDI, 4 districts/cities affected by TPT and HDI, 47 districts/cities affected by MSEs and HDI, and 3 districts/cities affected by TPT, UMK and HDI. The government can improve the quality of education, the level of public health services, and provide job training to reduce poverty.
Analysis of inclusive growth in ASEAN countries
Authors: Anindyntha Firdha Aksari 
Published: November 2023

There are 17 goals to achieve sustainable development in Sustainable Development Goals (SDGs) adopted by the United Nations. One of SDG that promote inclusive and sustainable economic growth is SDG number 8. Sustainable economic growth seeks to achieve goals that support the process of economic development. Inclusive growth is sought to support the process of economic growth and economic development to prevent future generations from suffering depletion of resources. The effort to achieve inclusive growth in line with the Sustainable Development Goals (SDGs). ASEAN was formed as an integration of any counties as a result of globalization,. The integrated economies of ASEAN countries form economic liberalization, giving rise to economic openness. The manifestation of economic openness is trade openness and financial openness. The panel regression method is used to see the effect of economic openness and macroeconomic variables on economic growth as a proxy for inclusive growth. The results found that inflation and unemployment as macroeconomic variables had a significant negative effect on economic growth. It means to achieve the inclusive growth; the ASEAN countries should keep the inflation stable and low unemployment rate.
Multi-harmonic structured illumination-based optical diffraction tomography
Authors: Liu Ruihua , Wen Kai , Li Jiaoyue , Ma Ying , Zheng Juanjuan , An Sha , Min Junwei , Zalevsky Zeev , Yao Baoli , Gao Peng 
Published: November 2023

Imaging speed and spatial resolution are key factors in optical diffraction tomography (ODT), while they are mutually exclusive in 3D refractive index imaging. This paper presents a multi-harmonic structured illumination-based optical diffraction tomography (MHSI-ODT) to acquire 3D refractive index (RI) maps of transparent samples. MHSI-ODT utilizes a digital micromirror device (DMD) to generate structured illumination containing multiple harmonics. For each structured illumination orientation, four spherical spectral crowns are solved from five phase-shifted holograms, meaning that the acquisition of each spectral crown costs 1.25 raw images. Compared to conventional SI-ODT, which retrieves two spectral crowns from three phase-shifted raw images, MHSI-ODT enhances the imaging speed by 16.7% in 3D RI imaging. Meanwhile, MHSI-ODT exploits both the 1st-order and the 2nd-order harmonics; therefore, it has a better intensity utilization of structured illumination. We demonstrated the performance of MHSI-ODT by rendering the 3D RI distributions of 5 µm polystyrene (PS) microspheres and biological samples.