bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reactivity, Syntheses, and Crystal Structures of Na5[MO2][X] with M: Co+, Ni+, Cu+; X: CO2‐3, SO2‐4, SO2‐3, S2‐ and Na25[CuO2]5[SO4]4[S].
Authors: Amann Peter , Moeller Angela 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Square‐Planar Coordinated Polyanions of Palladium, Platinum, and Gold Stannaborate [SnB11H11]2‐ Coordination Chemistry.
Authors: Marx Thiemo , Mosel Bernd , Pantenburg Ingo , Hagen Siegbert , Schulze Herbert , Wesemann Lars 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Ag13OsO6: A Silver Oxide with Interconnected Icosahedral Ag4+13 Clusters and Dispersed [OsO6]4‐ Octahedra.
Authors: Ahlert Sascha , Klein Wilhelm , Jepsen Ove , Gunnarsson Olle , Andersen Ole Krogh , Jansen Martin 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Employing the Structural Diversity of Nature: Development of Modular Dipeptide‐Analogue Ligands for Ruthenium‐Catalyzed Enantioselective Transfer Hydrogenation of Ketones.
Authors: Pastor Isidro M. , Vaestilae Patrik , Adolfsson Hans 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Biradicals/Zwitterions from Enallene‐Isonitriles. Formal [4 + 1] Cycloadditions Leading to 11H‐Indeno[1,2‐b]quinoline and Related Compounds.
Authors: Lu Xiaoling , Petersen Jeffrey L. , Wang Kung K. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Thermolysis of Symmetrical Dithiobiurea and Thioureidoethylthiourea Derivatives.
Authors: Hassan Alaa A. , Mourad Aboul‐Fetouh E. , El‐Shaieb Kamal M. , Abou‐Zied Ashraf H. , Doepp Dietrich 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Mild Oxidation of Alcohols with o‐Iodoxybenzoic Acid (IBX) in Ionic Liquid 1‐Butyl‐3‐methyl‐imidazolium Chloride and Water.
Authors: Liu Zhi , Chen Zhen‐Chu , Zheng Qin‐Guo 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Transformation of Thiocarbonyls to Their Corresponding Carbonyl Compounds Using n‐Butyltriphenylphosphonium Dichromate (BunPPh3)2Cr2O7 in Solution and under Microwave Irradiation.
Authors: Mohammadpoor‐Baltork I. , Memarian H. R. , Hajipoor A. R. , Bahrami K. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Tweaking Copper Hydride (CuH) for Synthetic Gain. A Practical, One‐Pot Conversion of Dialkyl Ketones to Reduced Trialkylsilyl Ether Derivatives.
Authors: Lipshutz Bruce H. , Caires Christopher C. , Kuipers Peter , Chrisman Will 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Diastereoselective Conjugate Addition to Chiral α,β‐Unsaturated Carbonyl Systems in Aqueous Media: An Enantioselective Entry to α‐ and γ‐Hydroxy Acids and α‐Amino Acids.
Authors: Suarez Rosa M. , Peres Sestelo Jose , Sarandeses Luis A. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Coupling of 3,8‐Dibromo‐1,10‐phenanthroline with 3,5‐Diethynylheptyloxybenzene: A Suzuki/Miyaura versus a Sonogashira Perspective.
Authors: Yang Jinhua , Oh Woon Su , Elder Ian A. , Leventis Nicholas , Sotiriou‐Leventis Chariklia 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Eco‐Friendly and Highly Chemoselective 1,3‐Oxathio‐ and 1,3‐Dithioacetalization of Aldehydes Using Ionic Liquids.
Authors: Yadav J. S. , Reddy B. V. S. , Kondaji G. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Simple, Effective and Highly Selective Cleavage of 3‐Methylbut‐2‐enyl (Prenyl) Ethers Using p‐Toluenesulfonic Acid.
Authors: Babu K. Suresh , Raju B. China , Srinivas P. V. , Rao A. Sridhar , Kumar S. Praveen , Rao J. Madhusudana 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
(S,S)‐2,3‐Dihydroxy‐2,3‐dihydrobenzoic Acid: Microbial Access with Engineered Cells of Escherichia coli and Application as Starting Material in Natural‐Product Synthesis.
Authors: Franke Dirk , Lorbach Volker , Esser Simon , Dose Christian , Sprenger Georg A. , Halfar Markus , Thoemmes Joerg , Mueller Rolf , Takors Ralf , Mueller Michael 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium‐Catalyzed Regiocontrolled and Stereoselective Alkylations of Bis(trifluoroethyl) Malonates with Dienyl Alcohols.
Authors: Takacs James M. , Jiang Xun‐tian , Leonov Alexei P. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bismuth(III) Nitrate Pentahydrate Bi(NO3)3×5H2O: An Inexpensive and Mild Reagent for the Efficient and Clean Oxidation of Thiols to Disulfides.
Authors: Khodaei M. M. , Mohammadpoor‐Baltork I. , Nikoofar K. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Homeopathic Ligand‐Free Palladium as a Catalyst in the Heck Reaction. A Comparison with a Palladacycle.
Authors: de Vries Andre H. M. , Mulders Jan M. C. A. , Mommers John H. M. , Henderickx Huub J. W. , de Vries Johannes G. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 2,5‐Bis[(3,4‐ethylenedioxy)thien‐2‐yl]‐3‐Substituted Thiophenes.
Authors: Pepitone Michael F. , Eaiprasertsak Kalya , Hardaker Stephen S. , Gregory Richard V. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A New Class of Optically Active Pyrrole Derivatives: (3R)‐3‐(Pyrrol‐1‐yl)alk‐1‐enes from D‐α‐Amino Acids.
Authors: Settambolo Roberta , Guazzelli Giuditta , Mengali Lucia , Mandoli Alessandro , Lazzaroni Raffaello 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Strategies for the Synthesis of N‐(Azacycloalkyl)bisindolylmaleimides: Selective Inhibitors of PKCβ.
Authors: Faul Margaret M. , Grutsch John L. , Kobierski Michael E. , Kopach Michael E. , Krumrich Christine A. , Staszak Michael A. , Udodong Uko , Vicenzi Jeffrey T. , Sullivan Kevin A. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
2‐Piperidin‐4‐yl‐benzimidazoles with Broad Spectrum Antibacterial Activities.
Authors: He Yun , Wu Baogen , Yang Jun , Robinson Dale , Risen Lisa , Ranken Ray , Blyn Lawrence , Sheng Suzie , Swayze Eric E. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
6‐Aryl‐pyrazolo[3,4‐b]pyridines: Potent Inhibitors of Glycogen Synthase Kinase‐3 (GSK‐3).
Authors: Witherington Jason , Bordas Vincent , Gaiba Alessandra , Garton Neil S. , Naylor Antoinette , Rawlings Anthony D. , Slingsby Brian P. , Smith David G. , Takle Andrew K. , Ward Robert W. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of a New Heterocycle, 1H‐4‐Aryl[1,2,4]oxadiazino[5,4‐b]quinazolinone (III)
Authors: Reddy L. Manmohan , Reddy P. Pratap , Reddy P. S. N. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Facile One‐Step Synthesis of a Thia‐Bridged Bis‐1,10‐phenanthroline Macrocycle.
Authors: Wang Wen‐Jwu , Sengul Abdurrahman , Luo Chi‐Feng , Kao Hsien‐Chang , Cheng Yi‐Hung 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Triethylborane‐Mediated Hydrogallation and Hydroindation: Novel Access to Organogalliums and Organoindiums.
Authors: Takami Kazuaki , Mikami Satoshi , Yorimitsu Hideki , Shinokubo Hiroshi , Oshima Koichiro 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel Heteroaromatic‐Based Multi‐Branched Dyes with Enhanced Two‐Photon Absorption Activity
Authors: Abbotto Alessandro , Beverina Luca , Bozio Renato , Facchetti Antonio , Ferrante Camilla , Pagani Giorgio A. , Pedron Danilo , Signorini Raffaella 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of a Novel Family of Diterpenes and Their Evaluation as Antiinflammatory Agents.
Authors: Lam Thanh , Ling Taotao , Chowdhury Chinmay , Chao Ta‐Hsiang , Bahjat F. R. , Lloyd G. K. , Moldawer Lyle L. , Palladino Michael A. , Theodorakis Emmanuel A. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of β‐Casomorphin Employing Fmoc‐Amino Acid Chlorides and t‐Butyldimethylsilyloxy Benzotriazole (TBDMS‐OBt).
Authors: Tantry Subramanyam J. , Mathad Raveendra I. , Babu Vommina V. Suresh 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Functionalized Organomagnesium Reagents Prepared Through Halogen—Metal Exchange
Authors: Knochel Paul , Dohle Wolfgang , Gommermann Nina , Kneisel Florian F. , Kopp Felix , Korn Tobias , Sapountzis Ioannis , Vu Viet Anh 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Multicomponent Coupling Reactions for Organic Synthesis: Chemoselective Reactions with Amide—Aldehyde Mixures
Authors: Jacobi von Wangelin Axel , Neumann Helfried , Goerdes Dirk , Klaus Stefan , Struebing Dirk , Beller Matthias 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
New Metal‐Rich Sulfides Ni6SnS2 and Ni9Sn2S2 with a 2D Metal Framework: Synthesis, Crystal Structure, and Bonding.
Authors: Baranov A. I. , Isaeva A. A. , Kloo L. , Popovkin B. A. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nine Hexagonal Ca5Pb3Z Phases in Stuffed Mn5Si3‐Type Structures with Transition Metal Interstitial Atoms Z. Problems with Classical Valence States in Possible Zintl Phases.
Authors: Guloy Arnold M. , Mudring Anja‐Verena , Corbett John D. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Inelastic Neutron Scattering and Raman Spectroscopies and Periodic DFT Studies of Rb2PtH6 and Rb2PtD6.
Authors: Parker Stewart F. , Bennington Stephen M. , Ramirez‐Cuesta Anibal. J. , Auffermann Gudrun , Bronger Welf , Herman Henryk , Williams Kenneth P. J. , Smith Tim 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of a New Chiral Receptor Containing 1,7‐Diaza‐12‐crown‐4 and Its Application in Chiral Separation.
Authors: Wang Cheng‐Yun , Shang Zhi‐Cai , Mei Jiang‐Hua , Yu Qing‐Sen 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Two‐Step Conversion of α,β‐Unsaturated Aldehydes to Optically Active γ‐Oxy‐α,β‐unsaturated Nitriles and Its Application to the Total Synthesis of (+)‐Patulolide C.
Authors: Tian Jun , Yamagiwa Noriyuki , Matsunaga Shigeki , Shibasaki Masakatsu 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Organocatalytic Asymmetric Domino Knoevenagel/Diels—Alder Reactions: A Bioorganic Approach to the Diastereospecific and Enantioselective Construction of Highly Substituted Spiro[5,5]undecane‐1,5,9‐triones.
Authors: Ramachary D. B. , Chowdari Naidu S. , Barbas Carlos III 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
N‐Chloro‐3‐methyl‐2,6‐diphenylpiperidin‐4‐one (NCP) — An Efficient, Mild, Stable Oxidant for Cleavage of Carbon—Nitrogen Double Bonds of Oximes.
Authors: Balasubramanian S. , Ramalingan C. , Kabilan S. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Antiviral Activity of Adamantyl‐Containing Aminoketones and their Derivatives.
Authors: Makarova N. V. , Boreko E. I. , Moiseev I. K. , Pavlova N. I. , Nikolaeva S. N. , Zemtsova M. N. , Savinova O. V. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Diastereoselective Oxy‐Michael Additions of Enantiopure δ‐Lactol Anions to Nitroalkenes: Asymmetric Synthesis of 1,2‐Amino Alcohols.
Authors: Adderley Nicola J. , Buchanan David J. , Dixon Darren J. , Laine Dramane I. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Resolution of (RS)‐Phenylglycinonitrile by Penicillin Acylase Catalyzed Acylation in Aqueous Medium.
Authors: Chilov Ghermes G. , Moody Harold M. , Boesten Wilhelmus H. J. , Svedas Vytas K. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A C(Aryl)—N(Amine) Bond Atropisomeric Aminophosphine: Preparation and Use as a Ligand in a Catalytic Asymmetric Allylic Alkylation.
Authors: Mino Takashi , Tanaka Youichi , Yabusaki Toshihiro , Okumura Daisuke , Sakamoto Masami , Fujita Tsutomu 
Published: June 2005

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Reactions of ortho‐Activated Anthra‐ and Naphthoquinones with Nitrite and Fluoride Ions.
Authors: Podvyazny O. V. , Sokolova M. S. , Churkina N. L. , Lavrikova T. I. , Gornostaev L. M. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of (.+‐.)‐trans‐2‐ [3‐Methoxy‐4‐(4‐chlorophenylthioethoxy)‐5‐ (N‐methyl‐N‐hydroxyureidyl)methylphenyl] ‐5‐(3,4,5‐trimethoxyphenyl)tetrahydrofuran.
Authors: Ram B. , Balram B. , Ram S. Raghu 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Direct Entry to the 1‐Methoxyindole Skeleton and to the Corresponding Indoles by a Novel Rearrangement: General Syntheses of Substituted 1‐Methoxyindoles.
Authors: Selvakumar N. , Reddy B. Yadi , Azhagan A. Malar , Khera Manoj Kumar , Babu J. Moses , Iqbal Javed 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
1‐N‐Substituted Thiocarbamoyl‐3‐phenyl‐5‐thienyl‐2‐pyrazolines: Synthesis and Evaluation as MAO Inhibitors.
Authors: Goekhan Nesrin , Yesilada Akguel , Ucar Guelberk , Erol Kevser , Bilgin A. Altan 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
[4 + 2]‐Cyclodimer of Sabinone: Formation, Crystal Structure, and NMR Spectra.
Authors: Suleimenov E. M. , Raldugin V. A. , Shakirov M. M. , Bagryanskaya I. Yu. , Gatilov Yu. V. , Kulyjiasov A. T. , Adekenov S. M. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Enantioselective Synthesis of cis‐ and trans‐3,5‐Disubstituted Piperidines. Synthesis of 20S‐ and 20R‐Dihydrocleavamine.
Authors: Amat Mercedes , Escolano Carmen , Lozano Oscar , Llor Nuria , Bosch Joan 
Published: June 2005

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Selenium‐Promoted Synthesis of Enantiomerically Pure Substituted Morpholines Starting from Alkenes and Chiral Aminoalcohols.
Authors: Tiecco Marcello , Testaferri Lorenzo , Marini Francesca , Sternativo Silvia , Santi Claudio , Bagnoli Luana , Temperini Andrea 
Published: June 2005

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Novel Stereoselective Synthesis of 1‐(β‐D‐ and α‐L‐Glycopyranosyl)‐5‐methyl‐1H‐1,2,4‐triazoles.
Authors: Yu Jianxin , Li Zhongjun , Lu Wenjie , Zhang Suna , Cai Mengshen 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Approaches to Intramolecular Sialylation. Part 1. Synthesis of Pseudodisaccharide Neu5Ac‐(1‐2)‐Gal with Ester‐Linked Monosaccharide Residues.
Authors: Kononov L. O. , Volodin D. A. , Magnusson G. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Variation in modes of chemotherapy administration for breast carcinoma and association with hospitalization for chemotherapy‐related toxicity
Authors: Du Xianglin L. , Chan Wenyaw , Giordano Sharon , Geraci Jane M. , Delclos George L. , Burau Keith , Fang Shenying 
Published: June 2005

AbstractBACKGROUNDTo the authors' knowledge, few studies to date have addressed the patterns of how chemotherapy was administered (administration modes) over time. In the current study, the goal of the authors was to describe how chemotherapy for breast carcinoma was administered and to determine whether chemotherapy administration modes were associated with toxicity in a community‐based large cohort.METHODSThe authors studied 5256 women who were diagnosed with breast carcinoma at age 65 years or older between 1992–1999 and received chemotherapy. The patients were identified from the Surveillance, Epidemiology, and End Results (SEER)Program–Medicare linked databases. Chemotherapy drugs and modes of administration were determined through procedure codes in Medicare claims.RESULTSOf the 5256 patients who received chemotherapy, 33% received it through an intravenous infusion for less than 1 hour; 39% through an intravenous infusion lasting 1–8 hours; 15% through an intravenous infusion lasting longer than 8 hours and requiring a pump; 12% through an intravenous push technique; and 1% through a subcutaneous, intramuscular, or intralesional injection. These modes varied substantially across the 11 SEER areas. The risks of hospitalization for chemotherapy‐related toxicities (neutropenia, fever, thrombocytopenia, and adverse effects of systemic therapy) were not found to be significantly associated with different modes of chemotherapy after adjusting for other factors. Compared with patients receiving 5‐flurouracil using an intravenous infusion for longer than 8 hours, the risk of toxicity was determined to be 0.96 (95% confidence interval [95% CI], 0.63–1.47) for patients treated with an intravenous infusion lasting 1–8 hours; 0.94 (95% CI, 0.62–1.41) for patients treated with an intravenous infusion lasting less than 1 hour; and 0.66 (95% CI, 0.38–1.08) for patients treated with subcutaneous, intramuscular, or intralesional injection or an intravenous push technique.CONCLUSIONSThere were substantial geographic variations noted in the modes of administering chemotherapy; however, these variations did not appear to be associated with the risk of toxicities (neutropenia, fever, thrombocytopenia, and adverse effects of systemic therapy). Cancer 2005. © 2005 American Cancer Society.
Effect of electric field pulses on the viability and on the membrane‐bound immunoglobulins of LPS‐activated murine B‐lymphocytes: Correlation with the cell cycle
Authors: Djuzenova Cholpon S. , Sukhorukov Vladimir L. , Klöck Gerd , Arnold W. Michael , Zimmermann Ulrich 
Published: June 2005

AbstractThe effects, of microsecond electropulses (1–kV/cm) on the viability of murine B lymphocytes and on their binding of antibodies by surface immunoglobulin (Ig) were studied in relation to the cell cycle. Before electropulsing, cultures given 48 h mitogenic stimulation showed at least two cell subpopulations, which were distinguishable by their levels of surface‐Ig expression as assessed with FITC‐labelled antibodies against mouse Ig. The immunofluorescence intensity of cells in S and G2/M phases was higher than that of G0/G1 cells.After exposure of the mitogen‐stimulated lymphocytes to three exponentially decaying (time constantτ=5–40 μs) electric field pulses, dye exclusion assay showed that pulsing at 1 or 2 kV/cm (at 4°C or 20°C) did not cause permeabilization. Field strengths of 3, 4, or 5 kV/cm resulted in 20%, 45%, or 70% of dye‐permeable cells, respectively, if the pulsed cells were transferred to phosphate‐buffered saline on ice for 30 min. Incubation in full medium at 37°C for 30 min (“resealing”) significantly decreased the percentage of permeabilized cells. Electropulsed G0/G1 cells were not only more resistant to direct electric exposure (tolerated higher field strengths) than S+G2/M cells but also responded better to resealing.The surface Ig of lymphocytes pulsed at higher fields and low temperature (4 or 5 kV/cm, τ =5 μs, three pulses, 4°C) was less easily immunostained than in controls or in cells pulsed at 2 kV/cm or less. At 5 kV/cm those cells that were not permeabilized showed a greater reduction in immunostaining, especially if resealed. © 1994 Wiley‐Liss, Inc.
Evaluation of testicular germ cell tumor proliferation using DNA flow cytometry and nucleolar organizer silver staining
Authors: Hasegawa Soichiro , Okamura Kikuo , Shimoji Toshio , Miyake Koji , Yamakawa Yoshinori , Sakamoto Hirofumi 
Published: June 2005

AbstractWe studied 41 testicular germ cell tumors (TGCT) using DNA flow cytometry and argyrophilic (silver staining) nucleolar organizer region (Ag‐NOR) quantitation to evaluate the proliferative characteristics of TGCT and the significance of those analytical techniques. In nonseminomatous germ cell tumor (NSGCT), the percentage of S + G2M phase cells (% S + G2M) in DNA aneuploid tumor was higher than in DNA of diploid tumors. Likewise, in NSGCT the aneuploid Ag‐NOR sites per nucleus (Ag‐NOR count) were significantly higher in DNA aneuploid than in DNA diploid tumors. There was also a significant linear correlation between % S + G2M phase cells and the Ag‐NOR count in TGCT. However, the DNA ploidy pattern and the % S + G2M phase cells, and Ag‐NOR counts, did not correlate significantly with the patient survival rate or clinical stage. In conclusion, our study suggests that Ag‐NOR enumeration is an effective technique for assessing cellular proliferative activity that is somewhat less expensive than DNA flow cytometry. © 1994 Wiley‐Liss, Inc.
Adsorption of aerosil on erythrocyte surface by flow cytometry measurements
Authors: Gerashchenko Bogdan I. , Gerashchenko Igor I. , Bogomaz Valery I. , Pantazis Cooley G. 
Published: June 2005

AbstractLight scattering properties of the erythrocytes in less than 0. 01% aerosil suspension were measured. It was shown that light scattering properties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are characterized by more intensive autofluorescence in the channel of green fluorescence than the erythrocytes. We propose that this observation allows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aerosil particles and the erythrocytes, was confirmed by photometric measurement of hemoglobin in the supernatant. © 1994 Wiley‐Liss, Inc.
Propidium iodide and the thiol‐specific reagent dacm as a dye pair for fluorescence resonance energy transfer analysis: An application to mouse sperm chromatin
Authors: Bottiroli G. , Croce A. C. , Pellicciari C. , Ramponi R. 
Published: June 2005

AbstractThe dyes N‐(‐7‐dimethylamino‐4‐methyl‐coumarinyl) maleimide and propidium iodide, specific for the thiol group and DNA, respectively, were considered as a donor‐acceptor couple suitable for investigating “in situ” the relative spatial distribution of DNA and protamines in mouse spermatozoa chromatin. The two dyes are characterized by favourable spectral properties, so that a simplified analytical procedure, based on the measurement of both donor and acceptor emission in double‐stained samples, can be applied to evaluate the relative efficiency of the energy transfer process and its topological distribution. The results obtained indicate that during the maturation process: 1) the basic arrangement of protamine‐DNA complex does not undergo structure changes, and 2) the oxidation of sulfhydryl to disulfide groups, resulting in chromatin stabilization, first involves the protamine thiols spatially closer to DNA. Fluorescence energy transfer imaging suggests that chromatin stabilization starts in the midportion of the sperm head, then spreads towards the periphery. © 1994 Wiley‐Liss, Inc.