bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Kinase Interacting Protein 1 regulates mitochondrial protein levels in energy metabolism and promotes mitochondrial turnover after exercise
Authors: Nijholt Kirsten T. , Sánchez-Aguilera Pablo I. , Mahmoud Belend , Gerding Albert , Wolters Justina C. , Wolters Anouk H. G. , Giepmans Ben N. G. , Silljé Herman H. W. , de Boer Rudolf A. , Bakker Barbara M. , Westenbrink B. Daan 
Published: November 2023

AbstractA Kinase Interacting Protein 1 (AKIP1) is a signalling adaptor that promotes mitochondrial respiration and attenuates mitochondrial oxidative stress in cultured cardiomyocytes. We sought to determine whether AKIP1 influences mitochondrial function and the mitochondrial adaptation in response to exercise in vivo. We assessed mitochondrial respiratory capacity, as well as electron microscopy and mitochondrial targeted-proteomics in hearts from mice with cardiomyocyte-specific overexpression of AKIP1 (AKIP1-TG) and their wild type (WT) littermates. These parameters were also assessed after four weeks of voluntary wheel running. In contrast to our previous in vitro study, respiratory capacity measured as state 3 respiration on palmitoyl carnitine was significantly lower in AKIP1-TG compared to WT mice, whereas state 3 respiration on pyruvate remained unaltered. Similar findings were observed for maximal respiration, after addition of FCCP. Mitochondrial DNA damage and oxidative stress markers were not elevated in AKIP1-TG mice and gross mitochondrial morphology was similar. Mitochondrial targeted-proteomics did reveal reductions in mitochondrial proteins involved in energy metabolism. Exercise performance was comparable between genotypes, whereas exercise-induced cardiac hypertrophy was significantly increased in AKIP1-TG mice. After exercise, mitochondrial state 3 respiration on pyruvate substrates was significantly lower in AKIP1-TG compared with WT mice, while respiration on palmitoyl carnitine was not further decreased. This was associated with increased mitochondrial fission on electron microscopy, and the activation of pathways associated with mitochondrial fission and mitophagy. This study suggests that AKIP1 regulates the mitochondrial proteome involved in energy metabolism and promotes mitochondrial turnover after exercise. Future studies are required to unravel the mechanistic underpinnings and whether the mitochondrial changes are required for the AKIP1-induced physiological cardiac growth.
Phyloproteomic study by MALDI-TOF MS in view of intraspecies variation in a significant homogenous phytopathogen Dickeya solani
Authors: Motyka-Pomagruk Agata , Babinska-Wensierska Weronika , Sledz Wojciech , Kaczorowska Anna-Karina , Lojkowska Ewa 
Published: November 2023

AbstractDickeya solani is an economically significant pectinolytic phytopathogen belonging to the Pectobacteriaceae family, which causes soft rot and blackleg diseases. Despite its notable impact on global potato production, there are no effective methods to control this pest. Here, we undertook a phyloproteomic study on 20 D. solani strains, of various origin and year of isolation, with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) supported by an in-depth characterization of the strains in terms of the virulence-associated phenotype. In spite of high homogeneity in this species, we herein revealed for the first time intraspecies variation in the MALDI-TOF MS protein profiles among the studied D. solani isolates. Finally, representative mass spectra for the four delineated clades are presented. A majority of the analysed D. solani strains showed high virulence potential, while two strains stood out in their growth dynamics, virulence factors production and ability to macerate plant tissue. Nonetheless, the metabolic profiles of D. solani strains turned out to be uniform, except for gelatinase activity. Given that all D. solani isolates distinctly grouped from the other Dickeya species in the MALDI-TOF MS analysis, there is strong evidence supporting the potential routine use of this method for fast and reliable to-species identification of D. solani isolates of environmental origin.
Exploring cross-cultural variations in visual attention patterns inside and outside national borders using immersive virtual reality
Authors: Šašinková Alžběta , Čeněk Jiří , Ugwitz Pavel , Tsai Jie-Li , Giannopoulos Ioannis , Lacko David , Stachoň Zdeněk , Fitz Jan , Šašinka Čeněk 
Published: November 2023

AbstractWe examined theories of cross-cultural differences in cognitive style on a sample of 242 participants representing five cultural groups (Czechia, Ghana, eastern and western Turkey, and Taiwan). The experiment involved immersive virtual environments consisting of two salient focal objects and a complex background as stimuli, which were presented using virtual reality headsets with integrated eye-tracking devices. The oculomotor patterns confirmed previous general conclusions that Eastern cultures have a more holistic cognitive style, while Western cultures predominantly have an analytic cognitive style. The differences were particularly noticeable between Taiwan and the other samples. However, we found that the broader cultural background of each group was perhaps just as important as geographical location or national boundaries. For example, observed differences between Eastern (more holistic style) and Western Turkey (more analytic style), suggest the possible influence of varying historical and cultural characteristics on the cognitive processing of complex visual stimuli.
An automated rat grimace scale for the assessment of pain
Authors: Arnold Brendan , Ramakrishnan Rahul , Wright Amirah , Wilson Kelsey , VandeVord Pamela J. 
Published: November 2023

AbstractPain is a complex neuro-psychosocial experience that is internal and private, making it difficult to assess in both humans and animals. In pain research, animal models are prominently used, with rats among the most commonly studied. The rat grimace scale (RGS) measures four facial action units to quantify the pain behaviors of rats. However, manual recording of RGS scores is a time-consuming process that requires training. While computer vision models have been developed and utilized for various grimace scales, there are currently no models for RGS. To address this gap, this study worked to develop an automated RGS system which can detect facial action units in rat images and predict RGS scores. The automated system achieved an action unit detection precision and recall of 97%. Furthermore, the action unit RGS classifiers achieved a weighted accuracy of 81–93%. The system’s performance was evaluated using a blast traumatic brain injury study, where it was compared to trained human graders. The results showed an intraclass correlation coefficient of 0.82 for the total RGS score, indicating that the system was comparable to human graders. The automated tool could enhance pain research by providing a standardized and efficient method for the assessment of RGS.
Genomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio)
Authors: Yilmaz Ozlem , Com Emmanuelle , Pineau Charles , Bobe Julien 
Published: November 2023

AbstractThe specific functions and essentiality of type II vitellogenin (Vtg2) in early zebrafish development were investigated in this study. A vtg2-mutant zebrafish line was produced and effects of genomic disturbance were observed in F2 females and F3 offspring. No change in vtg2 transcript has been detected, however, Vtg2 abundance in F2 female liver was 5×, and in 1 hpf F3 vtg2-mutant embryos was 3.8× less than Wt (p < 0.05). Fecundity was unaffected while fertilization rate was more than halved in F2 vtg2-mutant females (p < 0.05). Hatching rate was significantly higher in F3 vtg2-mutant embryos in comparison to Wt embryos. Survival rate declined drastically to 29% and 18% at 24 hpf and 20 dpf, respectively, in F3 vtg2-mutant embryos. The introduced mutation caused vitelline membrane deficiencies, significant mortalities at early embryonic stages, and morphological abnormalities in the surviving F3 vtg2-mutant larvae. Overrepresentation of histones, zona pellucida proteins, lectins, and protein degradation related proteins in F3 vtg2-mutant embryos provide evidence to impaired mechanisms involved in vitellin membrane formation. Overall findings imply a potential function of Vtg2 in acquisition of vitellin membrane integrity, among other reproductive functions, and therefore, its essentiality in early zebrafish embryo development.
AMMI an GGE biplot analysis of grain yield for drought-tolerant maize hybrid selection in Inner Mongolia
Authors: Li Yipu , Bao Haizhu , Xu Zhenghan , Hu Shuping , Sun Jiying , Wang Zhigang , Yu Xiaofang , Gao Julin 
Published: November 2023

AbstractDue to the ongoing global warming, maize production worldwide is expected to be heavily inflicted by droughts. The grain yield of maize hybrids is an important factor in evaluating their suitability and stability. In this study, we utilized the AMMI model and GGE biplot to analyze grain yield of 20 hybrids from the three tested environments in Inner Mongolia in 2018 and 2019, aiming at selecting drought-tolerant maize hybrids. AMMI variance analysis revealed highly significant difference on main effects for genotype, environment, and their interaction. Furthermore, G11 (DK159) and G15 (JKY3308) exhibited favorable productivity and stability across all three test environments. Moreover, G10 (LH1) emerged as the most stable hybrid according to the AMMI analysis and the GGE biplot. Bayannur demonstrated the highest identification ability among the three tested sites. Our study provides accurate identification for drought-resilient maize hybrids in different rain-fed regions. These findings can contribute to the selection of appropriate hybrids that exhibit productivity, stability, and adaptability in drought-prone conditions.
Incorporating ecosystem services into functional zoning and adaptive management of natural protected areas as case study of the Shennongjia region in China
Authors: Deng Zheng-yu , Cao Jia-shuo 
Published: November 2023

AbstractAgainst the background of global climate change and anthropogenic interference, studying the spatial and temporal heterogeneity of ecosystem services in important ecological function regions and rationally dividing the functional zones will help to promote the construction of the natural protected areas system dominated by national parks. The Shennongjia Region is an important candidate for China's national parks and one of the key pilots. Integrating the InVEST model, Getis-Ord Gi* index, hotspot analysis, GeoDetector and K-means clustering algorithm, we measured five ecosystem services interactions and delineate the functional zones. The results show that the spatial and temporal evolution of various ecosystem services in the Shennongjia Region between 2000 and 2020 was significant. All ecosystem services showed a decreasing and then increasing trend, except for carbon storage, which slowly declined. The ecological status of the region is in the process of polarization, with the local environment showed a trend of continuous deterioration. Water yield-habitat quality and carbon storage-water purification showed synergistic relationships; soil conservation showed trade-offs with water yield, carbon storage and water purification over a wide spatial range. The interaction between land surface temperature and vegetation cover was the most significant dominant factor. Hot spots for the comprehensive ecosystem services index were mainly located in the central and southern parts of the Shennongjia region and four types of ecosystem service functional zones were identified accordingly. This study is of great significance for maximizing the benefits of ecosystem service functions, the efficient allocation of environmental resources and the rational formulation of management policies in natural protected areas.
Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene
Authors: Sun Qi , Liu Diming , Cui Weiwei , Cheng Huimin , Huang Lixia , Zhang Ruihao , Gu Junlian , Liu Shuo , Zhuang Xiao , Lu Yi , Chu Bo , Li Jian 
Published: November 2023

AbstractRecent findings have shown that fatty acid metabolism is profoundly involved in ferroptosis. However, the role of cholesterol in this process remains incompletely understood. In this work, we show that modulating cholesterol levels changes vulnerability of cells to ferroptosis. Cholesterol alters metabolic flux of the mevalonate pathway by promoting Squalene Epoxidase (SQLE) degradation, a rate limiting step in cholesterol biosynthesis, thereby increasing both CoQ10 and squalene levels. Importantly, whereas inactivation of Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1), the branch point of cholesterol biosynthesis pathway, exhibits minimal effect on ferroptosis, simultaneous inhibition of both CoQ10 and squalene biosynthesis completely abrogates the effect of cholesterol. Mouse models of ischemia-reperfusion and doxorubicin induced hepatoxicity confirm the protective role of cholesterol in ferroptosis. Our study elucidates a potential role of ferroptosis in diseases related to dysregulation of cholesterol metabolism and suggests a possible therapeutic target that involves ferroptotic cell death.
An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging
Authors: Harada Tomoki , Hata Shoji , Takagi Rioka , Komori Takuma , Fukuyama Masamitsu , Chinen Takumi , Kitagawa Daiju 
Published: November 2023

AbstractPhototoxicity is an important issue in fluorescence live imaging of light-sensitive cellular processes such as mitosis. Among several approaches to reduce phototoxicity, the addition of antioxidants to the media has been used as a simple method. Here, we analyzed the impact of phototoxicity on the mitotic progression in fluorescence live imaging of human cells and performed a screen to identify the most efficient antioxidative agents that reduce it. Quantitative analysis shows that high amounts of light illumination cause various mitotic defects such as prolonged mitosis and delays of chromosome alignment and centrosome separation. Among several antioxidants, our screen reveals that ascorbic acid significantly alleviates these phototoxic effects in mitosis. Furthermore, we demonstrate that adding ascorbic acid to the media enables fluorescence imaging of mitotic events at very high temporal resolution without obvious photodamage. Thus, this study provides an optimal method to effectively reduce the phototoxic effects in fluorescence live cell imaging.
Mechanism of positive electret inhibition of Staphylococcus aureus biofilms
Authors: Wang Hongbao , Liang Hejuan , Guo Xin , Xu Jiajie , Jiang Jian , Sun Zhipeng , Liang Yuanyuan 
Published: November 2023

AbstractBacterial biofilm is an important factor in bacterial drug resistance. Recently, it has been proved that electret films can inhibit the bacterial biofilm, while its mechanism of action on biofilms is under further investigation. In this work, taking Staphylococcus aureus as an example, the inhibition of positive electret on bacterial biofilm was verified and its mechanism was explained. Two factors have been found to explain the inhibition mechanism of electret on bacterial biofilms. One is probably due to its inhibition of the expression of key genes related to bacterial biofilms induced by the electric field of positive electret, and the other is to prevent the aggregation of bacteria rather than the direct bactericidal effect. The conclusions are expected to be extended to other types of bacteria and expand the application of electrostatic materials in the field of biomedicine.
Enhancing Interoperability and Harmonisation of Nuclear Medicine Image Data and Associated Clinical Data
Authors: Fuchs Timo , Kaiser Lena , Müller Dominik , Papp Laszlo , Fischer Regina , Tran-Gia Johannes 
Published: November 2023

AbstractNuclear imaging techniques such as positron emission tomography (PET) and single photon emission computed tomography (SPECT) in combination with computed tomography (CT) are established imaging modalities in clinical practice, particularly for oncological problems. Due to a multitude of manufacturers, different measurement protocols, local demographic or clinical workflow variations as well as various available reconstruction and analysis software, very heterogeneous datasets are generated. This review article examines the current state of interoperability and harmonisation of image data and related clinical data in the field of nuclear medicine. Various approaches and standards to improve data compatibility and integration are discussed. These include, for example, structured clinical history, standardisation of image acquisition and reconstruction as well as standardised preparation of image data for evaluation. Approaches to improve data acquisition, storage and analysis will be presented. Furthermore, approaches are presented to prepare the datasets in such a way that they become usable for projects applying artificial intelligence (AI) (machine learning, deep learning, etc.). This review article concludes with an outlook on future developments and trends related to AI in nuclear medicine, including a brief research of commercial solutions.
Trochlear Ridge Prostheses for Reshaping Femoral Trochlear Ridges in Dogs with Patellar Luxation
Authors: Nicetto Tommaso , Longo Federico 
Published: November 2023

Abstract
Objectives To describe the implant characteristics and surgical application of a custom-made trochlear ridge prosthesis (TRP) and to report clinical outcomes in dogs affected by patellar luxation treated with TRP.
Study design Dogs affected by patellar luxation underwent computed tomography. A specific canine bone anatomical replica, a cutting guide, and a TRP were designed and provided for surgery. Surgical records, clinical and radiographic reassessments, complications, pre- and postoperative lameness, type and degree of patellar luxation, and TRP and patellar position after surgery were reviewed. Clinical outcomes were defined as full, acceptable, or unacceptable function.
Results The TRP was implanted in 60 femoral trochleae: 48 unilateral and 12 bilateral. Successful correction of patellar luxation was achieved in 59/60 cases. TRP was applied with other surgical techniques in 36/60 of the cases and as the only surgical procedure in 24/60 cases. Overall, three complications were observed: two minor and one major (patellar luxation recurrence). Neither implant loosening nor infection was observed. The mean radiographic follow-up was 3.8 months. At the time of the final follow-up, 57/60 cases were scored as fully functional.
Conclusion The TRP application either alone or in combination with other surgical techniques allowed for correction of patellar luxation and improvement in preoperative lameness with nominal complications. TRP could represent a potentially reliable alternative to trochleoplasty.
A Scoping Review of the Oral Microbiome in Preterm Infants
Authors: Koerner Rebecca , Bendixen Marion M. , Monk Angela , Lamberti Monica F.T. , Lorca Graciela L. , Neu Josef , Parker Leslie A. 
Published: November 2023

The purpose of this scoping review was to examine the oral microbiome composition in preterm infants, sampling and collection methods, as well as exposures associated with oral microbiome composition and health implications. We conducted a scoping review of the literature using the Arskey and O'Malley framework. We identified a total of 13 articles which met our inclusion criteria and purpose of this scoping review. Articles included in this review compared the oral microbiome in preterm infants to term infants, examined alterations to the oral microbiome over time, compared the oral microbiome to different body site microbiomes, and explored associations with clinically relevant covariates and outcomes. Exposures associated with the diversity and composition of the oral microbiome in preterm infants included delivery mode, oral feeding, oropharyngeal care, skin-to-skin care, and antibiotics. Day of life and birth weight were also associated with oral microbiome composition. The oral microbiome may be associated with the composition of the tracheal and gut microbiomes, likely due to their proximity. Alpha and beta diversity findings varied across studies as well as the relative abundance of taxa. This is likely due to the different sampling techniques and timing of collection, as well as the wide range of infant clinical characteristics. Multiple factors may influence the composition of the oral microbiome in preterm infants. However, given the heterogeneity of sampling techniques and results within this review, the evidence is not conclusive on the development as well as short- and long-term implications of the oral microbiome in preterm infants and needs to be explored in future research studies.
Key Points
Testing Race Differences in Risk of Gestational Diabetes Mellitus Associated with Household Food Insecurity (Using NHANES 2007–2018)
Authors: Gonzalez-Canas Carolina , Ding Qinglan , Simpson Vicki L. , Hass Zachary 
Published: November 2023

Objective This study aimed to estimate the association of household food insecurity with gestational diabetes mellitus (GDM) risk by race and ethnic group.
Study Design The study was a secondary analysis from the National Health and Nutrition Examination Survey from 2007 to 2018. A survey-weighted logistic regression model was constructed with self-reported GDM as the response. The primary independent variable was a four-level food security indicator, defined as the inability to obtain food in a socially acceptable way due to the lack of financial resources and controlled for several established risk factors. Analyses were stratified by race (White and Black) and ethnicity (Hispanic) to provide insight into how gestational diabetes risk differs by subpopulation.
Results Results indicated that family history of diabetes is a risk factor across all races (adjusted odds ratio [aOR]: 4.22–16.26), while household food insecurity is a significant risk factor for only Hispanic women living with a partner (aOR: 8.50 for very low food security).
Conclusion In the United States, Hispanic women's GDM risk may be uniquely impacted by food insecurity.
Key Points
Measurement of risk spillover effect based on EV-Copula method
Authors: Zhao Yuexu , Xu Weiqi 
Published: November 2023

AbstractBased on the extreme value theory, copula function, and conditional value at risk (Abbreviated as CoVaR) model, an extreme value copula CoVaR (EV-Copula CoVaR) model is established. In application, the risk spillover effect of the carbon trading market on the stock market of China is investigated. Firstly, using the index synthesis method, the carbon trading price index is synthesized through the price data of the test area of carbon emission, then the risk spillover effect of the carbon market is measured by the EV-Copula CoVaR, and the dynamic risk spillover ΔCoVaR of the carbon market to each stock market is investigated. Finally, the downside ΔCoVaR under different significance levels is measured, and the relationship between the self-risk and spillover risk of the carbon market is explored, the largest risk spillover effect to the stock market is the electricity market. The smaller the significance level, the greater the carbon market self-risk, and the greater the risk spillover of the carbon market to the stock market, which shows that there is a positive correlation between them.