bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRADITIONS OF WELCOMING BIRTH IN THE ENDE COMMUNITY, EAST NUSA TENGGARA: THE DIALECTICS OF ISLAM AND LOCAL CULTURE
Authors: Hidayah Hairul , Paris Ilman 
Published: February 2024

The tradition of welcoming a baby has an important role in the Ende people of East Nusa Tenggara. This study aims to understand how religious and traditional aspects are dialectic in welcoming the birth of a baby. This research method uses an ethnographic approach, involving participatory observation, in-depth interviews with traditional and religious leaders, as well as literature studies to gain a comprehensive understanding of the traditional practices and Islamic values ??involved. The results of the study show that the tradition of welcoming babies in the Ende people is a blend of Islamic teachings and local cultural values. Religious aspects such as special prayers are integrated with local practices.. Despite social and religious changes, this tradition continues to function as a social glue and cultural identity for the Ende people.
Development and Validation of Parent-Reported Gastrointestinal Health Scale in MECP2 Duplication Syndrome
Authors: Pehlivan Davut , Aras Sukru , Glaze Daniel G. , Ak Muharrem , Suter Bernhard , Motil Kathleen J. 
Published: February 2024

Abstract
Background/Aims:
We aimed to develop a validated patient-reported Gastrointestinal Health Scale (GHS) specific to MECP2 Duplication Syndrome (MDS) to be used in clinical trials.
Methods
MDS parents completed a Gastrointestinal Health Questionnaire (GHQ) to investigate the most relevant and important items associated with gastrointestinal problems in MECP2-related disorders. Item reduction was executed according to EORTC guidelines. We performed reliability and validity studies for the finalized scale.
Results
A total of 106 surveys were eligible for item reduction and validation processes. The initial 55 items were reduced to 38 items based on parent responses, expert opinion, and initial confirmatory factor analysis (CFA). The final MDS-specific GHS included 38 items and 7 factors that underwent further reliability and validity assessments. The power of the study was at least 0.982. The Cronbach’s alphas of the instruments were General Health: 0.799, Eating-Chewing-Swallowing: 0.809, Reflux: 0.794, Motility: 0.762, Mood: 0.906, Medication: 0.595, Parenting: 0.942 and all items together: 0.928. The correlation coefficient between total and individual item scores ranged from 0.215 to 0.730. Because of the ordinal nature of the variables, the diagonal weighted least squares estimation (DWLS) method was used to execute the CFA and Structural Equation Modeling. The GHS had excellent model fit with the acceptable range of fit indices values.
Conclusions
We developed a parent-reported, reliable, and valid MDS-specific GHS. This scale can be utilized in clinical settings or as an outcome measure in translational and clinical research.
Evolving drug discovery using AI, automation, and ASMS through an integrated D-preMTA-MTA strategy for target-focused library exploration
Authors: Ma Songling , Hu Chenming , Wang Tong , Shi Fangjun , Zhao Chuanfang , Wang Na , Yang Minghui , Yan Jun , Luo Jie , Yang Mingjun , Zhang Peiyu , Lai Lipeng , Ma Jian 
Published: February 2024

Abstract
In the realm of drug discovery, the Design-Make-Test-Analyses (DMTA) cycle plays a pivotal role, with the “M” phase often emerging as a bottleneck. Numerous endeavors have sought to expedite this phase, employing technologies like robotic arms, flow chemistry, and other devices for automated synthesis. This paper introduces “preMTA” as a game-changer, leveraging artificial intelligence (AI)-based molecular generative models, automated synthesis workflows, and the ASMS platform, creating the “D-preMTA-MTA” cycle. During the “preMTA” phase, AI-designed chemical spaces for specific targets are explored. Microgram-scaled target compounds are efficiently prepared through pooling reactions and streamlined work-ups via automated synthesis workflows. These compounds are then screened using ASMS to rank them based on competitive binding affinity. This strategic approach enables the subsequent MTA phase to focus on potent binders. Validation of this approach involved the discovery of novel inhibitors for T-cell protein tyrosine phosphatase (TCPTP or PTPN2). In the “D” phase, a target-focused library of 696 compounds was designed, with 140 strong binders swiftly identified in the “preMTA” phase. Subsequently, 51 of these compounds were scale-up synthesized and confirmed with IC50 values ranging from 16 nM to 277 nM, with 17 exhibiting IC50 values below 50 nM. The efficacy and seamless integration of each phase in the “D-preMTA-MTA” cycle can be attributed to a) the use of a synthesis-oriented molecular generative method streamlining automated synthesis, b) the deployment of a versatile and adaptable robotic arm capable of multitasking, and c) the implementation of ASMS screening methods reducing both synthetic and bio-testing complexities.
Essential oil and fenchone extracted from Tetradenia riparia (Hochstetter.) Codd (Lamiaceae) induce oxidative stress in Culex quinquefasciatus larvae (Diptera: Culicidae) without causing lethal effects on non-target animals
Authors: de Oliveira André C. , Simões Rejane C. , Silva Felipe M. A. da , Sá Ingrity S. C. , Costa Maria L. L. da , Nunomura Sergio M. , Nunomura Rita C. S. , Roque Rosemary A. 
Published: February 2024

Abstract

We investigated the larvicidal activity of the essential oil (EO) from Tetradenia riparia and its majority compound fenchone for controlling Culex quinquefasciatus larvae, focusing in reactive oxygen and nitrogen species (RONS), catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) activities, and total thiol content as oxidative stress indicators. Moreover, the lethal effect of EO and fenchone was evaluated against Anisops bouvieri, Diplonychus indicus, Danio rerio, and Paracheirodon axelrodi. The EO and fenchone (5 to 25 µg/mL) showed larvicidal activity (LC50 from 16.05 to 18.94 µg/mL), followed by an overproduction of RONS, and changes in the activity of CAT, GST, AChE and total thiol content. The Kaplan-Meier followed by Log-rank (Mantel-Cox) analyses showed a 100% survival rate for A. bouvieri, D. indicus, D. rerio, and P. axelrodi when exposed to EO and fenchone (262.6 and 302.60 µg/mL), while α-cypermethrin (0.25 µg/mL) was extremely toxic to these non-target animals, causing 100% of death. These findings emphasize that the EO from T. riparia and fenchone serve as suitable larvicides for controlling C. quinquefasciatus larvae, without imposing lethal effects on the non-target animals investigated.
Modelling Efficient Fire Safety Water Networks by Certified Domination
Authors: Raczek Joanna , Miotk Mateusz 
Published: February 2024

Abstract

This paper explores a new way of designing water supply networks for fire safety using ideas from graph theory, focusing on a method called certified domination. Ensuring a good water supply is crucial for fire safety in communities. This study looks at the rules in Poland for how much water and pressure is needed to fight fires in different buildings and areas. We present a way to plan water supply networks for fire protection as a graph, where each point (node) is a place that needs water, and the lines (links) show where water can go between these points. The main idea is to find the best places to put pumping stations and wells in the network to save money and still meet all the fire safety requirements. Our approach assumes that it costs more to build a pumping station than a well. We use some examples to show how this method can find cost-effective solutions for water supply networks, while ensuring that they meet fire safety requirements and are not too expensive to build. This approach is a new and efficient way to improve the design of water supply networks for fire safety. Key challenges that are solved in this paper are a linear lime algorithm finding an optimal solution for networks without cycles and a BLP (Binary Linear Programming) algorithm solving the problem in arbitrary networks.
Early radical lung cancer outcome after prompt recovery from COVID-19 infection: A real-world study
Authors: Ma Ran , Ge Yong , Feng Tianci , Sun Teng , Feng Shoujie , Zhang Hao 
Published: February 2024

Abstract
Background:
There has been an increase in the number of patients with lung cancer who had previously contracted SARS-CoV-2 and currently require surgery. This study was to share the early postoperative outcomes in patients who underwent radical lung cancer surgery after early recovery of COVID-19 infection.
Methods
We retrospectively collected data for 99 patients who had undergone lung cancer surgery in our hospital during January 2022 and January 2023 (including the peak of the COVID-19 crisis）. The patients were divided into two groups according to the inclusion criteria and exclusion criteria. One of these included individuals with a history of SARS-CoV-2 infection. Perioperative and follow-up data at 30-day and 90-day were recorded.
Results
There were no statistical differences between groups (p > 0.05) in terms of their postoperative complications or 30-day and 90-day postoperative readmission rates. However, there were significant differences between groups (p < 0.05) in terms of their tumor sizes, pathological stages, total drainage volumes, drainage diversion times, and hospital stays.
Conclusions
The results of the present trial suggested that it is safe to implement radical curative lung cancer surgery in patients without pulmonary impairment and in the early stages (2–4 weeks) of recovery from SARS-CoV-2 infection.
Synergistic effects of bioactive peptide and cytarabine on leukemia cells
Authors: Suyila Qimuge , Sun Jiaxing , Li Xian , Su Xiulan 
Published: February 2024

Abstract
Purpose
Acute leukemia is caused by the malignant cloning of primitive bone marrow naive cells in vivo. This study aimed to explore the effects of anti-cancer bioactive peptides (ACBP) on leukemia cell proliferation, apoptosis, and peripheral blood lymphocytes in leukemia mice and to assess the combined effects of ACBP and the chemotherapy drug cytarabine (Ara-C) on acute leukemia.
Materials and methods
Cell proliferation and viability were measured using CCK-8. Apoptosis and cell cycle were measured by flow cytometry, and Caspase 3/7 protein expression was measured using an IncuCyte Live cell workstation. BALB/c mice were inoculated intraperitoneally to establish a P388 acute lymphatic leukemia cell model. Mouse peripheral blood lymphocyte subsets (Th, Ts, B, and NK cells) were detected by flow cytometry after ACBP, Ara-C, and ACBP/Ara-C treatment, and the levels of cytokines (TNF-α, IFN-r, IL-2, IL-4, IL-6, and IL-10) were also assessed.
Results
The results showed that ACBP effectively inhibited the proliferation of human myeloid leukemia mononuclear (THP-1) cells. The combined regimen also significantly induced apoptosis in THP-1 cells, upregulated Caspase3/7 expression, and blocked cell cycle in G2/M phase. Further ACBP/Ara-C activated Th, Ts, and B cells in P388 leukemic mice, upregulated of IFN-r and IL-2, downregulation of TNF- α, and IL-6.
Conclusion
These findings suggest that ACBP inhibits leukemia cell proliferation by activating the Caspase 3/7 pathway, arresting the cell cycle, activating lymphocyte subsets, promoting the secretion of cytokines, and activating immune functions. The synergistic effects of ACBP and Ara-C present new options for the clinical treatment of leukemia.
Expression and Significance of the GDF11 Protein in Ureteropelvic Junction Obstruction (UPJO)
Authors: Lao Weihua , Shi Tong , Chen Qin , Qiao Bao , Ma Sai , Liu Cuiwei 
Published: February 2024

Abstract

Background
To explore the expression of growth differentiation factor 11 (GDF11), a member of the TGF-β superfamily, in pediatric ureteropelvic junction obstruction (UPJO) patients.
Methods
Five patients diagnosed with severe neonatal hydronephrosis underwent laparoscopic pyeloplasty between January 2021 and November 2022. Tissue samples from the constricted ureteropelvic junction and adjacent normal ureteral segments served as controls. The presence of the GDF11 protein in these tissues was examined using immunohistochemistry and Western blot techniques. Subsequently, a statistical analysis compared the protein expression levels in both groups.
Results
Immunohistochemical staining revealed that the frequency of high GDF11 protein expression in the narrowed ureter (60%) was significantly greater than that in the normal ureter (40%). Western blot analysis indicated that the expression of GDF11 was significantly greater in the narrowed ureter than in the normal ureter.
Conclusions
The increased expression of GDF11 in UPJO is noteworthy and deserves clinical attention.
Comparison of cytokine expression and disease severity between plasma cell-dominant and eosinophil-dominant patients in chronic rhinosinusitis with nasal polyps
Authors: Lin Yu-Tsai , Tsai Ming-Hsien , Su Yan-Ye , Huang Shun-Chen 
Published: February 2024

Abstract
Purpose
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a heterogeneous disease characterized by inflammation of the nasal and sinus mucosa. The inflammatory patterns may differ among patients, leading to different subtypes based on the dominant inflammatory cell type. This study aimed to compare the differences in cytokine expression and disease severity between plasma cell-dominant and eosinophil-dominant subtypes in patients with CRSwNP.
Methods
This study included 53 CRSwNP patients and 19 control subjects who did not have asthma or a history of cigarette smoking. The expression of Th2 cytokines (IL-4, IL-5, IL-6, and IL-13) and inflammatory cells was assessed via enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively.
Results
Among the cytokines analyzed, only IL-6 was significantly different between the two subtypes. A greater proportion of mast cells and IgE cells was present in plasma cell-dominant CRSwNP patients than in eosinophil-dominant group. For the three disease severity scores (LMK-CT, TPS and SNOT-22), objective scores (LMK-CT and TPS) were greater in the eosinophil-dominant CRSwNP group, while the opposite result was shown for the subjective score (SNOT-22). Additionally, the percentage of plasma cell-dominant cells was significantly positively correlated with disease severity according to the TPS and SNOT-22 scores.
Conclusions
Our data revealed that plasma cell-dominant inflammation, a subtype of type 2 CRS, was significantly correlated with subjective disease severity. The study also highlights the role of IL-6, IgE and mast cells as distinguishing factors between eosinophil-dominant and plasma cell-dominant CRSwNP. This information could be useful for clinical diagnosis and personalized treatment.
Management of patients with Long Covid: A qualitative study exploring the roles of nurses in healthcare pathways
Authors: KAMDEM Linda , GUYOT Jessica , DUPRE Caroline , GOUTTEFARDE Pauline , VERICEL Marie Pierre , FANGET Marie , NKENFOU Céline , HUPIN David , ROCHE Frederic , BOTELHO-NEVERS Elisabeth , BONGUE Bienvenu 
Published: February 2024

Abstract

Aim
Investigate the various roles played by nurses in the care of patients afflicted with Long COVID.
Background
Effectively managing Long Covid requires a multidisciplinary approach - a healthcare pathway that necessitates collaboration among various members of the medical profession to monitor the patient. Among these professions, nursing plays a crucial role. This article compiles information on how nurses are involved in the care of patients afflicted with Long Covid: What roles do they play in enhancing the care of these patients? Are these roles distinct from those they perform in other chronic conditions?
Methods
We conducted a qualitative study among healthcare professionals in France and enrolled eighteen participants in our study. Semi-structured interviews were conducted with professionals working across various care sectors in France, including private practice, hospitals, schools, and research. A thematic content analysis was performed, and emerging themes were subsequently discussed until the most significant categories were identified. This study was conducted in accordance with the COREQ checklist.
Results
Nurses play a wide range of roles within their practices, depending on their practice settings. For instance, a nurse practitioner may work in a range of settings such as hospital outpatient clinics, private group practices, inpatient units, or urgent care units. Depending on their work environment, nurses' roles within healthcare pathways may encompass screening and guidance, clinical patient monitoring, providing relational support, patient education, collaborative care and coordination as well as involvement in research.
Conclusion
The predominant role identified in our study involves coordinating the management of the Long Covid syndrome. The next step would be the implementation of a city-hospital Long Covid healthcare pathway.
Implication for the Nursing & Health Policy Perspectives
Nursing work is difficult to specify, as it includes numerous recognised and unrecognised aspects. The results of this study highlight a new essential role which is that of coordinating the health pathways of patients suffering from Long Covid.
In silico characterization of bla OXA -carrying plasmids in Escherichia coli
Authors: Wu Jie , Liu Mengyue , Zhao Jiaxue , Xi Yanyan , Yang Haiyan , Chen Shuaiyin , Long Jinzhao , Duan Guangcai 
Published: February 2024

Abstract
To comprehensively understand the association between plasmids and blaOXA genes in Escherichia coli (E. coli), the variants of blaOXA, antimicrobial resistance genes (ARGs), host Sequence Types, conjugative transfer regions and geographic distribution of 328 blaOXA-positive plasmids in E. coli were investigated. The 328 plasmids were found to carry 341 blaOXA genes belonging to 12 types of blaOXA variants. blaOXA−1 (n = 190) and blaOXA−10 (n = 37) were the most prevalent blaOXA variants, followed by blaOXA−48 (n = 35) and blaOXA−181 (n = 33). The blaOXA−1 variant was mainly carried by the IncF plasmids, while the majority of blaOXA−10 was located on IncH plasmids in E. coli. The former was widely distributed worldwide, while the latter was mainly concentrated in Asia. In E. coli, the blaOXA−48 gene was mainly carried by IncL replicons, which was mainly isolated in Spain and Switzerland. Multi-locus sequence typing (MLST) analysis showed that ST2, ST43, and ST471 accounted for the majority of E coli carrying blaOXA-positive plasmids, which deserved long-term attention in monitoring system. ARGs analysis results showed that ARGs was highly diverse in E. coli plasmids carrying blaOXA, suggesting that the E. coli harboring blaOXA has been gradually developing into the super-bacteria. These findings demonstrate the importance of plasmids in OXA β-Lactamases transmission and propose the importance of monitoring these factors to better understand and control the occurrence of antibiotic resistance.
Production study of Fr, Ra and Ac radioactive ion beams at ISOLDE, CERN
Authors: Jajčišinová E. , Dockx K. , Au M. , Bara S. , Cocolios T. E. , Chrysalidis K. , Farooq-Smith G. J. , Fedorov D. V. , Fedosseev V. N. , Flanagan K. T. , Heines M. , Houngbo D. , Johnson J. D. , Kellerbauer A. , Kraemer S. , Marsh B. A. , Popescu L. , Ramos J. P. , Rothe S. , Seliverstov M. D. , Sels S. , Stegemann S. , Stryjczyk M. , Verelst V. 
Published: February 2024

Abstract
The presented paper discusses the production of radioactive ion beams of francium, radium, and actinium from thick uranium carbide (UC x) targets at ISOLDE, CERN. This study focuses on the release curves and extractable yields of francium, radium and actinium isotopes. The ion source temperature was varied in order to study the relative contributions of surface and laser ionization to the production of the actinium ion beams. The experimental results are presented in the form of release parameters. Representative extractable yields per µC are presented for 222−231 Ac, several Ra and Fr isotopes in the mass ranges 214≤A≤233 and 205≤A≤231 respectively. The release efficiency for several isotopes of each of the studied elements was calculated by comparing their yields to the estimated in-target production rates modeled by CERN-FLUKA. The maximal extraction efficiency of actinium was calculated to be 2.1(6)% for a combination of surface ionization using a Ta ion source and resonant laser ionization using the two-step 438.58 nm, and 424.69 nm scheme.
Distinct characteristics and changes in liver function of patients with HCC treated with Ate/Bev for more than 1 year
Authors: Kim Youngun , Kim Jung Sun , Kang Beodeul , Kim Ilhwan , Kim Hyeyoung , Lee Won Suk , Sang Yun Beom , Jung Sanghoon , An Chansik , Kim Chan , Chon Hong Jae 
Published: February 2024

Abstract
Background/Aims:Since 2020, Atezolizumab plus bevacizumab (Ate/Bev) has been the standard first-line therapy for unresectable hepatocellular carcinoma (HCC), but long-term treatment studies are limited. This study evaluated the clinical characteristics and effects of Ate/Bev for over 1 year.
Methods: This study included patients with unresectable HCC treated with first-line Ate/Bev between May 2020 and April 2022. Those receiving Ate/Bev for 1 year or more were classified as the long-term treatment group.
Results:Of 246 patients, 69 (28.0%) were in the long-term treatment group. This group had more proportions of intrahepatic tumor burden <25%, ECOG 0, and a lower proportion of portal vein tumor thrombosis than the short-term treatment group. The long-term treatment group had a higher incidence of atezolizumab-related thyroid dysfunction (31.9% vs. 10.7%, p<0.001; median time to onset [mTTO]: 2.8 months), dermatologic toxicity (29.0% vs. 14.7%, p=0.017; mTTO: 3.3 months), bevacizumab-related hypertension (44.9% vs. 22.0%, p=0.001; mTTO: 4.2 months), and proteinuria (69.6% vs. 38.4%, p<0.001; mTTO: 6.8 months), compared to the short-term treatment group. Regarding liver function in the long-term treatment group, patients initially classified as Child–Pugh class A decreased from 87% to 75.4%, and albumin-bilirubin grade 1 decreased from 68.1% to 50.7% after 1 year of treatment.
Conclusions:The Ate/Bev long-term treatment group had a lower intrahepatic tumor burden, less portal vein tumor thrombosis, and better performance status and liver function at baseline. Atezolizumab-related immunological adverse events emerged relatively early in treatment compared to the bevacizumab-related. Additionally, some patients showed liver function deterioration during long-term Ate/Bev treatment.