bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ‐SATB2 (hsa_circ_0008928) and miR‐150‐5p are regulators of TRIM66 in the regulation of NSCLC cell growth and metastasis of NSCLC cells via the ceRNA pathway
Authors: Chen Liangji , Zhou Yuting , Cheng Hongbing , Lu Wenjing , Cai Mengyang , Jiang Kaifeng 
Published: December 2023

AbstractCircular RNA (circRNA) was an important modulator and potential molecular target of nonsmall cell lung cancer (NSCLC). CircSATB2 was reported to be upregulated in NSCLC. However, the role and mechanism of circSATB2 in NSCLC progression remain to be illustrated. The RNA and protein expression was detected by quantitative real‐time polymerase chain reaction, western blot, and immunohistochemistry assay. Cell counting kit‐8, cell colony formation, and 5‐ethynyl‐2′‐deoxyuridine assays were applied to assess cell growth. The migrated and invaded cells were examined by transwell assay. Flow cytometry was performed to measure apoptotic cells. The interaction among circSATB2, microRNA‐150‐5p (miR‐150‐5p), and tripartite motif‐containing protein 66 (TRIM66) was identified by dual‐luciferase reporter assay and RNA immunoprecipitation assay. An in vivo experiment was conducted to investigate the effect of circSATB2 on tumor growth. CircSATB2 expression was highly expressed in NSCLC tissues and cell lines. CircSATB2 and TRIM66 silencing both suppressed NSCLC cell growth, migration, and invasion whereas promoted NSCLC cell apoptosis. CircSATB2 acted as a molecular sponge for miR‐150‐5p, and miR‐150‐5p interacted with the 3′ untranslated region (3′UTR) of TRIM66. Moreover, circSATB2 knockdown‐induced effects were partly reversed by TRIM66 overexpression in NSCLC cells. Besides, cirSATB2 expression was negatively correlated with miR‐150‐5p level and positively correlated with TRIM66 level in NSCLC tumor tissues. CircSATB2 knockdown blocked xenograft tumor growth in vivo. In summary, this study verified that circSATB2 stimulated NSCLC cell malignant behaviors by miR‐150‐5p/TRIM66 pathway, providing a possible circRNA‐targeted therapy for NSCLC.
Amino acids and their roles in tumor immunotherapy of breast cancer
Authors: Xia Hongzhuo , Zhu Jianyu , Zheng Zhuomeng , Xiao Peiyao , Yu Xiaohui , Wu Muyao , Xue Lian , Xu Xi , Wang Xinyu , Guo Yuxuan , Zheng Chanjuan , Ding Siyu , Wang Yian , Peng Xiaoning , Fu Shujun , Li Junjun , Deng Xiyun 
Published: December 2023

AbstractBreast cancer is the most commonly diagnosed cancer among women. The primary treatment options include surgery, radiotherapy, chemotherapy, targeted therapy and hormone therapy. The effectiveness of breast cancer therapy varies depending on the stage and aggressiveness of the cancer, as well as individual factors. Advances in early detection and improved treatments have significantly increased survival rates for breast cancer patients. Nevertheless, specific subtypes of breast cancer, particularly triple‐negative breast cancer, still lack effective treatment strategies. Thus, novel and effective therapeutic targets for breast cancer need to be explored. As substrates of protein synthesis, amino acids are important sources of energy and nutrition, only secondly to glucose. The rich supply of amino acids enables the tumor to maintain its proliferative competence through participation in energy generation, nucleoside synthesis and maintenance of cellular redox balance. Amino acids also play an important role in immune‐suppressive microenvironment formation. Thus, the biological effects of amino acids may change unexpectedly in tumor‐specific or oncogene‐dependent manners. In recent years, there has been significant progress in the study of amino acid metabolism, particularly in their potential application as therapeutic targets in breast cancer. In this review, we provide an update on amino acid metabolism and discuss the therapeutic implications of amino acids in breast cancer.
Rainbow subgraphs in edge‐colored complete graphs: Answering two questions by Erdős and Tuza
Authors: Axenovich Maria , Clemen Felix C. 
Published: December 2023

AbstractAn edge‐coloring of a complete graph with a set of colors is called completely balanced if any vertex is incident to the same number of edges of each color from . Erdős and Tuza asked in 1993 whether for any graph on edges and any completely balanced coloring of any sufficiently large complete graph using colors contains a rainbow copy of . This question was restated by Erdős in his list of “Some of my favourite problems on cycles and colourings.” We answer this question in the negative for most cliques by giving explicit constructions of respective completely balanced colorings. Further, we answer a related question concerning completely balanced colorings of complete graphs with more colors than the number of edges in the graph .
Complex I, V, and MDH2 deficient human skin fibroblasts reveal distinct metabolic signatures by 1H HR‐MAS NMR
Authors: Meyer Christoph , Hertig Damian , Arnold Janine , Urzi Christian , Kurth Sandra , Mayr Johannes A. , Schaller André , Vermathen Peter , Nuoffer Jean‐Marc 
Published: December 2023

AbstractIn this study, we investigated the metabolic signatures of different mitochondrial defects (two different complex I and complex V, and the one MDH2 defect) in human skin fibroblasts (HSF). We hypothesized that using a selective culture medium would cause defect specific adaptation of the metabolome and further our understanding of the biochemical implications for the studied defects. All cells were cultivated under galactose stress condition and compared to glucose‐based cell culture condition. We investigated the bioenergetic profile using Seahorse XFe96 cell analyzer and assessed the extracellular metabolic footprints and the intracellular metabolic fingerprints using NMR. The galactose‐based culture condition forced a bioenergetic switch from a glycolytic to an oxidative state in all cell lines which improved overall separation of controls from the different defect groups. The extracellular metabolome was discriminative for separating controls from defects but not the specific defects, whereas the intracellular metabolome suggests CI and CV changes and revealed clear MDH2 defect‐specific changes in metabolites associated with the TCA cycle, malate aspartate shuttle, and the choline metabolism, which are pronounced under galactose condition.
Tooth pattern, development, and replacement in the yellow catfish, Pelteobagrus fulvidraco
Authors: Huang Rui , Tang Li , Li Ruiqi , Li Yongfeng , Zhan Liping , Huang Xiaofeng 
Published: December 2023

AbstractStudies of teleost teeth are important for understanding the evolution and mechanisms of tooth development, replacement, and regeneration. Here, we used gross specimens, microcomputed tomography, and histological analysis to characterize tooth structure, development, and resorption patterns in adult Pelteobagrus fulvidraco. The oral and pharyngeal teeth are villiform and conical. Multiple rows of dentition are densely distributed and the tooth germ is derived from the epithelium. P. fulvidraco exhibits a discontinuous and non‐permanent dental lamina. Epithelial cells surround the teeth and are separated into distinct tooth units by mesenchymal tissue. Tooth development is completed in the form of independent tooth units. P. fulvidraco does not undergo simultaneous tooth replacement. Based on tooth development and resorption status, five forms of teeth are present in adult P. fulvidraco: developing tooth germs, accompanied by relatively immature tooth germs; mature and well‐mineralized tooth accompanied by one tooth germ; teeth that have begun resorption, but not completely fractured; fractured teeth with only residual attachment to the underlying bone; and teeth that are completely resorbed and detached. Seven biological stages of a tooth in P. fulvidraco were also described.
Prevalence and risk factors of anal human papillomavirus infection among men with anal condyloma acuminata by HIV status in ShenZhen, Southeast China: A retrospective cohort study
Authors: Liu Jiaxin , Yang Rongqing , Huang Jie , Zhang Mingshuang , Zhao Xiaobao , Chu Wenzhu , Wei Lanlan 
Published: December 2023

AbstractPatients with anal condyloma acuminatum (CA) are at risk of developing anal cancer which is associated with oncogenic human papillomavirus (HPV) infection. Investigation of anal HPV prevalence and risk factors can provide effective strategies for the prevention of anal cancer. A retrospective study was conducted among 549 patients with anal CA in the Third People's Hospital of Shenzhen between January 2019 and October 2021. HPV prevalence and HIV antibodies were detected by fluorescent PCR and ELISA, respectively. Logistic regression model and structural equation modeling (SEM) were conducted to analyzed the risk factors of oncogenic HPV infection. The overall prevalence of HPV was 96.72%. Both HPV6 (N = 285, 51.91%) and HPV11 (N = 300, 54.64%) were more than half infected and the most frequent Hr‐HPV genotype was HPV16 (N = 138, 25.14%). HIV‐positive (AOR: 5.02, 95% CI: 2.98−8.60, p < 0.0001) and history of syphilis (AOR: 4.24, 95% CI: 2.31−8.46, p < 0.0001) were independent risk factors statistically associated with oncogenic HPV infection. Ever had anal sex (AOR: 3.40, 95% CI: 1.28−11.81, p = 0.0267) and age 35 years and older (AOR: 2.79, 95% CI: 1.53−5.15, p = 0.0009) were associated with HPV16 and HPV52, respectively. SEM analyses showed that HIV‐positive (b = 1.549, p < 0.001) and history of syphilis (b = 1.450, p < 0.001) had significant positive effects on oncogenic HPV infection. Ever had anal sex (b = 1.243, p = 0.025) and Age (b = 0.043, p = 0.002) positively drived HPV16 and HPV52 infection, respectively. Anal CA patients who are HIV‐positive, have a history of syphilis, or at least 35 years old should be considered for Hr‐HPV, cytology and other anal cancer related tests to reduce the risk of cancer development.
Diffusion kurtosis imaging reveals abnormal gray matter and white matter development in some brain regions of children with attention‐deficit/hyperactivity disorder
Authors: Tang Shilong , Liu Xianfan , Nie Lisha , Qian Fangfang , Chen Wushuang , He Ling , Yang Mei 
Published: December 2023

AbstractIn this study, we explored the application of diffusion kurtosis imaging (DKI) technology in the brains of children with attention‐deficit/hyperactivity disorder (ADHD). Seventy‐two children with ADHD and 79 age‐ and sex‐matched healthy controls were included in the study. All children were examined by means of 3D T1‐weighted image, DKI, and conventional sequence scanning. The volume and DKI parameters of each brain region were obtained by software postprocessing (GE ADW 4.6 workstation) and compared between the two groups of children to determine the imaging characteristics of children with ADHD. The result showed the total brain volume was lower in children with ADHD than in healthy children (p < .05). The gray and white matter volumes in the frontal lobe, temporal lobe, hippocampus, caudate nucleus, putamen, globus pallidus, and other brain regions were lower in children with ADHD than in healthy children (p < .05). The axial kurtosis (Ka), mean kurtosis (MK), fractional anisotropy (FA), and radial kurtosis(Kr) values in the frontal lobe, temporal lobe, and caudate nucleus of children with ADHD were lower than those of healthy children, while the mean diffusivity(MD) and fractional anisotropy of kurtosis (FAK) values were higher than those of healthy children (p < .05). Additionally, the Ka, MK, FA, and Kr values in the frontal lobe, caudate nucleus, and temporal lobe could be used to distinguish children with ADHD (AUC > .05, p < .05). In conclusion, DKI showed abnormal gray matter and white matter development in some brain regions of children with ADHD.
Evolution of ENSO‐related seasonal precipitation anomalies in East Asia: A robustness revisit
Authors: Liu Yunyun , Hu Zeng‐Zhen , Wu Renguang , Ding Yihui , Jha Bhaskar 
Published: December 2023

AbstractThe El Niño–Southern Oscillation (ENSO) is a major source of seasonal climate predictability and a key predictor for East Asian precipitation. However, the impacts of ENSO on East Asian precipitation are extremely complex and still controversial. Thus, it is necessary and significant to use various (observational and model) data sources to further examine the robustness of the impacts. This study revisits the evolution of the ENSO‐related seasonal precipitation anomalies in East Asia, including symmetric and asymmetric impacts of El Niños and La Niñas, the differences in the influences of eastern Pacific (EP) and central Pacific (CP) El Niños, and the interdecadal change of ENSO influences. The ENSO impact on East Asian precipitation has weakened (strengthened) in the ENSO's developing (decay) phase in the recent two decades. The ENSO impacts are robust in (i) southern China‐lower reaches of the Yangtze River‐southern Japan around the ENSO's mature phase, (ii) western North China in the developing phase and (iii) southwestern China in the decay phase. The distribution of East Asian precipitation anomalies is asymmetric in El Niño and La Niña years. The model simulations forced with observed sea surface temperature generally reproduce the observed evolution of seasonal precipitation anomalies associated with ENSO and confirm the robust impact of ENSO in East Asia. These results indicate the necessity to consider the asymmetric impact of El Niños and La Niñas, to distinguish the El Niño flavours for their impact on the East Asian climate, and to include the interdecadal variation of ENSO influence in addressing East Asian climate anomalies.
Clinical outcomes of ABCB4 heterozygosity in infants and children with cholestatic liver disease
Authors: Hegarty Robert , Gurra Olivia , Tarawally Jenneh , Allouni Sammi , Rahman Obydur , Strautnieks Sandra , Kyrana Eirini , Hadzic Nedim , Thompson Richard J. , Grammatikopoulos Tassos 
Published: December 2023

AbstractObjectivesBiallelic variants in the adenosine triphosphate binding cassette subfamily B member 4 (ABCB4) gene which encodes the multidrug resistance 3 protein (MDR3) leads to progressive familiar intrahepatic cholestasis type 3. However, monoallelic variants are increasingly recognized as contributing to liver disease in adults. Our aim was to describe the clinical characteristics of MDR3 heterozygous variants in a large cohort of infants and children with cholestatic liver disease.MethodsThe clinical and genotypic data on pediatric patients seen at King's College Hospital, London, between 2004 and 2022 and found to harbour heterozygous variants in ABCB4 were reviewed.ResultsNinety‐two patients amongst 1568 tested were identified with a monoallelic variant (5.9%). The most common presenting problem was conjugated hyperbilirubinemia (n = 46; 50%) followed by cholelithiasis (n = 12; 13%) and cholestatic hepatitis (n = 10; 11%). The median values of liver biochemistry at presentation were: GGT 105 IU/L and total bilirubin 86 µmol/L. Thirty‐two genetic variants were identified including 22 missense (69%), 4 deletions (13%), 5 splice site (16%) and 1 termination (3%). At a median follow up of 1 year there was resolution of liver disease.ConclusionsRare variants in ABCB4 were found amongst infants and children with cholestatic liver disease. The presenting problems were variable and abnormalities tended to normalize over time. Those with severe mutations could develop liver disease later in life when exposed to further insult and should be counseled appropriately.
Examining the functional space of gut microbiome‐derived peptides
Authors: Lee Ying‐Chiang J. 
Published: December 2023

AbstractThe human gut microbiome contains thousands of small, novel peptides that could play a role in microbe–microbe and host–microbe interactions, contributing to human health and disease. Although these peptides have not yet been systematically characterized, computational tools can be used to elucidate the bioactivities they may have. This article proposes probing the functional space of gut microbiome‐derived peptides (MDPs) using in silico approaches for three bioactivities: antimicrobial, anticancer, and nucleomodulins. Machine learning programs that support peptide and protein queries are provided for each bioactivity. Considering the biases of an activity‐centric approach, activity‐agnostic tools using structural and chemical similarity and target prediction are also described. Gut MDPs represent a vast functional space that can not only contribute to our understanding of microbiome interactions but potentially even serve as a source of life‐changing therapeutics.
Uncommon/rare oncogenic drivers in non‐small cell lung cancer: Consensus and contention
Authors: Wu Yi‐Long , Lu Shun , Cheng Ying , Zhou Qing , Tu Hai‐Yan , Zhou Qing‐Hua , Wang Lv‐Hua , Zhang Li , Zhou Jian‐Ying , Huang Cheng , Chen Ming , Hu Cheng‐Ping , Chuai Shao‐Kun , Wang Xiao‐Nan , Liu Xiao‐Qing , Liu Ji‐Wei , Zhou Peng‐Hui , Chen Wei‐Zhi , Yan Ling‐Hua , Liu Yun‐Peng , Liu An‐Wen , Zhang Xu‐Chao , Li Hui , Chen Rong‐Rong , Lin Dong‐Mei , Xie Cong‐Ying , Zhu Zheng‐Fei , Liang Hui‐Ying , Song Yong , Dong Xiao‐Rong , Zhao Ming‐Fang , Qiao Gui‐Bin , Cui Jiu‐Wei , Li Zi‐Ming , Wang Zhi‐Jie , Chen Xiao‐Yuan , Yang Nong , Lin Gen , Tian Pan‐Wen , Fan Yun , Song Qi‐Bin , Chen Yuan , Duan Jian‐Chun , Wang Jia‐Lei , Zhu Bo , Wang Bu‐Hai , Zhao Jun , Yu Qi‐Tao , Wang Li‐Feng , Zhang Hai‐Bo , Hu Jie , Ma Rui , Zhang Tong‐Mei , Lin Jie , Chu Qian , Ren Sheng‐Xiang , Yao Yu , Wu Lin , Wang Hui‐Juan , Wu Fang , Zhong Wen‐Zhao , Hu Yi , Chen Ke‐Neng , Zhao Jian , Zhang Li , Yang Fan , Wang Qun , Yue Dong‐Sheng , Zhou Jian‐Ya , Shen Peng , Zhang Jia‐Tao , Yan Xiao‐Long , Huang Mei‐Juan , Feng Wei‐Neng , Li Li ,   Chinese Association of Lung Cancer, Guangdong Association of Clinical Trials (GACT)/Chinese Thoracic Oncology Group (CTONG)
Published: December 2023

AbstractThe importance of uncommon/rare oncogenic drivers in non‐small cell lung cancer (NSCLC) was underscored during the 20th China Lung Cancer Summit. These drivers, while present in a significant proportion of NSCLC patients, remain a challenge for diagnosis and therapeutic targeting. In the never‐smokers/low smokers category with mutations such as EGFR and HER2, the efficacy of immune checkpoint inhibitors (ICIs) remains suboptimal, attributed to lower PD‐L1 expression and tumor mutation burden (TMB). However, heavy smokers, often with mutations like KRAS, may derive benefits from ICIs, as supported by trials like CheckMate‐057. With the complex landscape of these drivers and their clinical implications, the summit culminated in six pivotal consensus points, aiming to guide future research and clinical decisions. Despite the advancements, the detection, interpretation, and therapeutic strategies involving these drivers necessitate further exploration and standardization.
Compact dual‐band bandpass filter based on EMSICC and CPW resonator with excellent design flexibility
Authors: Fang Ruihong , Guan Xuehui , Ren Baoping , Zhang Xiaoyan , Liu Xinlei 
Published: December 2023

AbstractIn this letter, a novel dual‐band bandpass filter (BPF) with independently controllable center frequencies, bandwidths, and transmission zeros (TZs) is presented. The proposed BPF is realized by using eighth‐mode substrate‐integrated circular cavities (EMSICCs) and coplanar waveguide (CPW) resonators. The CPW resonators are embedded in the upper surface of the EMSICCs, allowing for a compact size without additional circuit dimensions. Finally, a dual‐band BPF for WLAN is designed and fabricated with center frequencies of 2.45 and 5.2 GHz. The measured results closely align with the simulated ones, validating the performance of the filter. Multiple TZs are generated by cross‐coupling and source‐load coupling, which results in good out‐of‐band rejection performance.
The restricted SAR protocol: A method to assess MRI coil prototypes in an unconditionally safe manner
Authors: Dudysheva Natalia , Mauconduit Franck , Abdeddaim Redha , Gapais Paul‐François , Hosseinnezhadian Sajad , Dubois Marc , Amadon Alexis , Boulant Nicolas , Hertz‐Pannier Lucie , Vignaud Alexandre 
Published: December 2023

AbstractPurposeTesting an RF coil prototype on subjects involves laborious verifications to ensure its safety. In particular, it requires preliminary electromagnetic simulations and their validations on phantoms to accurately predict the specific absorption rate (SAR). For coil design validation with a simpler safety procedure, the restricted SAR (rS) mode is proposed, enabling representative first experiments in vivo. The goal of the developed approach is to accelerate the transition of a custom coil system from prototype to clinical use.MethodsThe restricted specific absorption rate (SAR) (rS) mode imposes a radical limitation on the transmitted RF power based on a worst‐case scenario of local RF power absorption. The limitations used are independent of the SAR spatial distribution, making this approach unconditionally safe. The developed rS protocol contains the sequences required for coil evaluation and satisfies the imposed rS conditions. It provides a quantitative characterization of the coil transmission and reception profiles and a qualitative evaluation of the anatomical images. Protocol validation was performed on commercial and pre‐industrial prototype coils on a small cohort of healthy volunteers.ResultsThe proposed rS protocol enables coil evaluation within an acquisition time compatible with common clinical protocol duration. The total time of all evaluation steps does not exceed 17 min. At the same time, the global SAR remains 100 times less than the International Electrotechnical Commission safety limit for played sequences.ConclusionThe rS protocol allows characterizing and comparing coil prototypes on volunteers without extensive electromagnetic calculations and phantom validations in an unconditionally safe way.
Nutrition support management of organ transplant recipients in the acute posttransplant phase
Authors: Hahn Michaelann , Wood Abby , Hasse Jeanette M. 
Published: December 2023

AbstractPatients who undergo solid organ transplant can have an extensive and challenging postoperative course. The chronicity of the disease state prior to transplant in combination with transplant‐specific complications and immunosuppressant medications can lead to distinct challenges that are not observed in other critically ill patients. Although the manifestation of posttransplant complications may be specific to the organ being transplanted, there are common transplant challenges that affect nutrition therapy in these patients. Effects of malnutrition, metabolic aberrations, and posttransplant organ dysfunction should be considered when developing a nutrition care plan for patients in the immediate posttransplant phase. This article addresses the various complications that can arise in the immediate posttransplant phase among patients undergoing solid organ transplant and the appropriate nutrition interventions or considerations for this specialized patient population.