bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of UPLC-MS/MS Method for Simultaneous Determination of Multi-Class Antibiotics, Antivirals and Antifungals in Water
Authors: KONIDALA SATHISH KUMAR , SHANMUGAM S SIVA , NARAYANAN S. , SHANDIL R.K. , PURAM PAVAN KUMAR , SAMINENI RAMU , PULUSU VEERA SHAKAR , SRI KAMMA HARSHA 
Published: September 2023

According to a WHO report, antimicrobial resistance is one of the top 10 health threats. Environmental sources are extremely contaminated with high levels of antibiotics, antivirals and antifungals due to their excessive and unsafe usage. Hence, there is a need to detect and quantitate the antimicrobial agents in water. Consequently, a simultaneous analytical method is developed and validated, employing a solid-phase extraction methodology with an HLB cartridge. This method was utilized to quantify a minimum of twenty different chemicals derived from antibiotics, antivirals and antifungal drugs. Antibiotics include quinolones, isonicotinic acid, macrolides, oxazolidinones, penicillin, sulphonamides, antivirals that contain nucleotide class compounds and antifungals that comprising of triazoles and imidazoles. Ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC- S/MS) was used to quantify the analytes. Detection was achieved using multiple reaction monitoring with positive electron spray ionization. All the compounds were well separated with the column, Xterra MS C18 4.6 × 50 mm 2.5 μm, using water and acetonitrile with 0.1% formic acid as a mobile phase. The calibration curve range was set at 50 to 2000 ng mL-1. Validation parameters like precision and accuracy, matrix effect, sensitivity, autosampler stability, the limit of detection and recovery were performed.
Quem são os “Líderes Escolares”? Condições de gestão entre Japão, Alemanha e Reino Unido
Authors: Tsujino Kemma , Suematsu Hiroki 
Published: September 2023

Neste estudo, são analisadas as estruturas de administração e gestão das escolas públicas no Japão, na Alemanha e no Reino Unido. Visto que existe uma lacuna considerável entre o sistema e a prática nesses países, assim, "Quem são os diretores das escolas?" pode constituir um tema de investigação não só importante, mas também urgente. Essa questão implica a importância de se concentrar na estrutura global do sistema de ensino público para além de uma "unidade" escolar. A reforma radical da gestão escolar é uma das agendas comuns entre estes países. A análise de documentos e o trabalho de campo são realizados a partir de perspectivas comuns a estes três países, tais como "reforma escolar", "autonomia escolar", "diferenças/semelhanças". Como resultado, são clarificadas as condições de gestão escolar e as estruturas de governança nestes três países. Embora os diretores japoneses tenham formal e legalmente mais autoridade para serem dirigentes escolares, a base substancial para a gestão escolar não é suficiente, existindo muitas limitações. Para compreender os "dirigentes escolares" alemães, há que analisá-los em relação aos princípios da "supervisão estatal da escola", da "autonomia escolar" e da "participação educativa" dos professores, pais e alunos. No Reino Unido, as decisões em matéria de finanças, pessoal e currículo são tomadas pelo órgão diretivo. Com base na tomada de decisões, os diretores assumem o papel de Diretor Executivo. A estrutura de governança distribuída existe no ensino público em cada país, e há diversos atores que dirigem as escolas com diferentes responsabilidades.
Pension Reform and Improved Employment Protection: Effects on Older Men’s Employment Outcomes
Authors: Kajitani Shinya , Kan Mari 
Published: September 2023

Abstract
This study examines the impacts of demand-side (the improved employment protection law) and supply-side (pension reform) government interventions on older male workers’ employment outcomes in Japan. To identify the effects of interventions implemented concurrently, we employ a difference-in-difference-in-differences (DDD) approach with variation in the bindingness of the revision of employment protection law as our specification strategy. Our results show that the revised employment protection law had a significant positive impact on employment. However, the results of the event study’s DDD model show that the treatment effects do not last over time.
A portable household detection system based on the combination of bidirectional LSTM and residual block for automatical arrhythmia detection
Authors: Huang Zeqiong , Yang Shaohua , Zou Qinhong , Gao Xuliang , Chen Bin 
Published: September 2023

Abstract

Objectives
Arrhythmia is an important component of cardiovascular disease, and electrocardiogram (ECG) is a method to detect arrhythmia. Arrhythmia detection is often paroxysmal, and ECG signal analysis is time-consuming and expensive. We propose a model and device for convenient monitoring of arrhythmia at any time.

Methods
This work proposes a model combining residual block and bidirectional long-term short-term memory network (BiLSTM) to detect and classify ECG signals. Residual blocks can extract deep features and avoid performance degradation caused by convolutional networks. Combined with the feature of BiLSTM to strengthen the connection relationship of the local window, it can achieve a better classification and prediction effect.

Results
Model optimization experiments were performed on the MIT-BIH Atrial Fibrillation Database (AFDB) and MIT-BIH Arrhythmia Database (MITDB). The accuracy simulation results on both long and short signal was higher than 99 %. To further demonstrate the applicability of the model, validation experiments were conducted on MIT-BIH Normal Sinus Rhythm Database (NSRDB) and the Long-Term AF Database (LTAFDB) datasets, and the related recognition accuracy were 99.830 and 91.252 %, respectively. Additionally, we proposed a portable household detection system including an ECG and a blood pressure detection module. The detection accuracy was higher than 98 % using the collected data as testing set.

Conclusions
Hence, we thought our system can be used for practical application.
Rescaling creatinine makes GFR estimation equations generally applicable across populations – validation results for the Lund-Malmö equation in a French cohort of sub-Saharan ancestry
Authors: Nyman Ulf , Björk Jonas , Delanaye Pierre , Lahens Alexandre , Pottel Hans , Vidal-Petiot Emmanuelle , Flamant Martin 
Published: September 2023

Abstract

Objectives
To make glomerular filtration rate (GFR) estimating equations applicable across populations with different creatinine generation by using rescaled serum creatinine (sCr/Q) where sCr represents the individual creatinine level and Q the average creatinine value in healthy persons of the same population.

Methods
GFR measurements (mGFR, plasma clearance of 51Cr-EDTA) were conducted in 964 adult Black Europeans. We established the re-expressed Lund-Malmö revised equation (r-LMR) by replacing serum creatinine (sCr) with rescaled creatinine sCr/Q. We evaluated the r-LMR equation based on Q-values of White Europeans (r-LMRQ-white; Q-values females: 62 μmol/L, males: 80 μmol/L) and Black Europeans (r-LMRQ-Black; Q-values females: 65 μmol/L, males: 90 μmol/L), and the European Kidney Function Consortium equation (EKFCQ-White and EKFCQ-Black) regarding bias, precision (interquartile range, IQR) and accuracy (percentage of estimates within ±10 % [P10] and ±30 % [P30] of mGFR).

Results
Median bias of r-LMRQ-White/r-LMRQ-Black/EKFCQ-White/EKFCQ-Black were −9.1/−4.5/−6.3/−0.9 mL/min/1.73 m2, IQR 14.7/14.5/14.5/15.6 mL/min/1.73 m2, P10 25.1 %/34.8 %/30.3 %/37.2 % and P30 74.2 %/84.1 %/80.6 %/83.6 %. The improvement of bias and accuracy when using proper Q-values was most pronounced in men. Similar improvements were obtained above and below mGFR 60 mL/min/1.73 m2 and at various age and BMI intervals, except for BMI<20 kg/m2 where bias increased, and accuracy decreased.

Conclusions
GFR estimating equations may be re-expressed to include rescaled creatinine (sCr/Q) and used across populations with different creatinine generation if population-specific average creatinine concentrations (Q-values) for healthy persons are established.
Insights From Informal Research Mentoring of Three University EFL Teachers
Authors: Yan Chunmei , Gong Wuming , Zou Chunyan , Li Jun 
Published: September 2023

Abstract
Research-practice nexus has been an ideal for higher education. However, research mentoring has been underexplored in both formal and informal formats. This study aimed to examine the effects of one-year-long informal research mentoring conducted by an experienced EFL teacher educator for three teaching-focused EFL teachers from three different universities. It found that the informal research mentoring generated three positive effects. It fueled the mentees’ research interest, raised their awareness of self-discipline and stress-coping strategies, and kindled their enthusiasm for constructing a collegial environment in their working contexts. Meanwhile, limitations also emerged associated with the mentees’ limited research engagement and productivity owing to personal and contextual factors as well as the problems with the informal mentoring relationships. Implications of the study are drawn to improve faculty research mentoring processes and outcomes.
Establishment of two oxaliplatin-resistant gallbladder cancer cell lines and comprehensive analysis of dysregulated genes
Authors: Guo Haijun , Zhi Yunqing , Wang Kaijing , Li Na , Yu Danlei , Ji Zhonghua , Chen Bo 
Published: September 2023

Abstract
Acquired resistance to chemotherapeutic drugs in gallbladder cancer (GBC) results in therapy failure. This study is aimed to establish oxaliplatin (OXA)-resistant GBC cell lines and uncover their gene expression profiles. First, two OXA-resistant GBC cell lines (GBC-SD/OXA and SGC996/OXA) were established by gradually increasing the drug concentration, and the resistance index was 4–5. The two resistant cell lines showed slower proliferation and higher stemness, colony formation, and migration abilities. Epithelial mesenchymal transformation and increased levels of P-glycoprotein were also detected. Next RNA-sequence analysis identified 4,675 dysregulated genes (DGs) in resistant cells, and most of the 12 randomly selected DGs were verified to be consistent with the sequence results. Kyoto Encyclopedia of Genes and Genomes analysis indicated that several DGs were involved in resistance- and phenotype-related pathways, of which the activations of PD-L1 and ERK1/2 were both verified in resistant cell lines. In conclusion, this study is the first to report the gene expression profile of OXA-resistant GBC cells and provides a useful database for target development.
Broadband large-angle beam scanning with dynamic spin energy distribution based on liquid crystal cascaded bilayer metasurface
Authors: Zhao Huijun , Liu Jiayue , Jiang Songlin , Jiang Xinhao , Cheng Jierong , Ji Yunyun , Chang Shengjiang , Fan Fei 
Published: September 2023

Abstract
Dynamic manipulation of terahertz (THz) beams plays an important role in THz application systems. The PB metasurface provides an effective scheme for space separation and deflection of the spin beam. However, mirror symmetry locking of the conjugated spin states severely limits the versatility of the device. In this work, we demonstrate a liquid crystal (LC) cascaded bilayer metasurface that includes an LC layer, anisotropic metasurface, and PB metasurface. By controlling anisotropy and polarization conversion effects, dynamic spin asymmetric transmission is realized. Meanwhile, two different dynamic energy distribution processes are realized between the L and R state with the corresponding deflection side. The results show that the device achieves a large angular spatial dispersion within the frequency-angle scanning range of ±35° to ±75° corresponding to the broadband range of 0.6–1.1 THz. Moreover, it achieves a spin beam spatial separation with a maximum proportion of energy distribution greater than 26 dB, and the active modulation rate in the energy distribution process reaches 98 %. This work provides a dynamic THz spin conversion and efficient large-angle beam scanning, with important potentials in wavelength/polarization division multiplexing and frequency-scanning antenna for large-capacity THz wireless communication, radar, and imaging systems.
Diversity and structure of bacterial communities in saline and non-saline rice fields in Cilacap Regency, Indonesia
Authors: Hindersah Reginawanti , Pratiwi Etty , Fatma Yuli Siti , Simarmata Tualar 
Published: September 2023

Abstract
Increased concerns for sustainable agriculture have led to increased use of beneficial rhizobacteria as biofertilizers. Soil bacteria play a significant role in the nutrient cycle of soil, but their presence can be affected by abiotic stress, such as salinity. This study aimed to compare the chemical characteristics of slightly saline and non-saline rice soil and examine the bacterial community structure in both rhizosphere and bulk soil. We utilized 16SrRNA gene sequencing and performed arithmetic means clustering, a type of hierarchical clustering, on the samples collected from the rice fields of Cimrutu and Rawaapu Village in Cilacap Regency, Indonesia. Although the nutrient content was similar in both soils, there was a noticeable difference in their electrical conductivity (EC) despite the two locations being less than 4 km apart. The EC value in the Cimrutu soil suggests that it is non-saline, while the Rawaapu soil exhibits a low salinity level. The study found that Proteobacteria was the most prevalent phylum in saline rhizospheric soil. In contrast, Firmicutes was the most abundant group in saline bulk soil and non-saline rhizospheric and bulk soil. Additionally, Halothiobacillus, Thioalkalispira-Slvurivermis, and Acidothermus genera dominated the saline rhizospheric soil, suggesting that halotolerant microbes play a significant role as plant growth-promoting rhizobacteria in saline soil. The study provides valuable insights into cultured or uncultured bacterial populations and structure in saline and non-saline soil to develop future strategies related to salinity by introducing beneficial microbes.
Domestic Hybrids: Vitruvius’ Xenia, the Surrealist’s Minotaure, and Shrigley’s Octopus
Authors: Weir Simon 
Published: September 2023

Abstract
The domestic spaces of the built environment are traditionally associated with residential architecture. But the domestic spaces can also extend out, metaphorically, into familiar public spaces in which one may feel at home, and also extend inwards into self-perception, insofar as you may say that you dwell within yourself. This article begins by recalling Vitruvius’ fundamental notion of architectural utilitas concerns accommodating not a building’s owners but foreigners and strange outsiders. Vitruvius’ view on utility heavily favoured architecture’s socio-political function, and the guests he believed that architecture ought to accommodate were not merely a home’s owners or their visiting friends, but those people who are more distant from a home’s owners: those who are stranger and less well understood, known as xenos and who ought to be respected under the Ancient Greek religious and ethical principle of xenia. It is on these grounds that Vitruvius makes an ethical critique of residential architecture in favour of the virtue of public architecture. Next the reach of xenia is proposed to extend towards those who are different not merely because of ethnic differences but cognitive and sensory differences. Such accommodations are today accounted for as part of accessibility design and salutogenic design. Similar conceptions are noted in Nietzsche’s notion of an “architecture for the perceptive” and the surrealist’s interpretation of the minotaur as a hybrid not only of animal and human but a hybrid of civilised citizen and barbarian outsider. Together these sketch out an expanded sense of the domestic that includes public spaces designed to accommodate strange outsiders and the hybrid forms used to signify them.
La mise à l’essai du programme « T’ES prêt! » au Cégep de Jonquière : ce qu’en pensent les enseignantes en éducation spécialisée
Authors: Harvey Stéphanie , Tardif Suzie , Pouliot Eve 
Published: September 2023

Pour mieux répondre aux besoins de leurs étudiants en situation de handicap, le département d’éducation spécialisée a participé à une recherche-action-formation permettant la conception et la mise à l’essai du programme « T’ES prêt ». Dans ce contexte, des entrevues qualitatives semi-dirigées ont été réalisées auprès des enseignantes (n=4) qui ont animé les activités du programme afin de connaitre leur point de vue sur ce dernier. Les résultats montrent que les enseignantes avaient des appréhensions reliées aux exigences que demande l’implantation d’un tel programme, mais soulèvent cinq éléments qui ont facilité son déploiement.
Ectopic Cushing’s syndrome from a corticotropin-releasing hormone-secreting medullary thyroid carcinoma: a rare pitfall of inferior petrosal sinus sampling
Authors: Mäkinen Vivi-Nelli , Horskær Madsen Stine , Ji Riis-Vestergaard Mette , Bjerre Mette , Bønløkke Pedersen Steen , Asa Sylvia L , Rolighed Lars , Lunde Jørgensen Jens Otto , Ornstrup Marie Juul 
Published: September 2023

Summary
This case report describes a rare presentation of ectopic Cushing’s syndrome (CS) due to ectopic corticotropin-releasing hormone (CRH) production from a medullary thyroid carcinoma (MTC). The patient, a 69-year-old man, presented with symptoms of muscle weakness, facial plethora, and easy bruising. An inferior petrosal sinus sampling test (IPSS) demonstrated pituitary adrenocorticotrophic hormone (ACTH) secretion, but a whole-body somatostatin receptor scintigraphy (68Ga-DOTATOC PET/CT) revealed enhanced uptake in the right thyroid lobe which, in addition to a grossly elevated serum calcitonin level, was indicative of an MTC. A 18F-DOPA PET/CT scan supported the diagnosis, and histology confirmed the presence of MTC with perinodal growth and regional lymph node metastasis. On immunohistochemical analysis, the tumor cell stained positively for calcitonin and CRH but negatively for ACTH. Distinctly elevated plasma CRH levels were documented. The patient therefore underwent thyroidectomy and bilateral adrenalectomy. This case shows that CS caused by ectopic CRH secretion may masquerade as CS due to a false positive IPSS test. It also highlights the importance of considering rare causes of CS when diagnostic test results are ambiguous.

Learning points

Medullary thyroid carcinoma may secrete CRH and cause ectopic CS.

Ectopic CRH secretion entails a rare pitfall of inferior petrosal sinus sampling yielding a false positive test.

Plasma CRH measurements can be useful in selected cases.
Piecemeal Resection for Large Colorectal Adenomas Remains Essential in 2022: A Single-Center Experience in a Tertiary French Center
Authors: Ratone Jean-Philippe , Archimbaud Clément , Solovyev Alexey , Zemmour Christophe , Pesenti Christian , Hoibian Solène , Dahel Yanis , Marx Mariola , De Chaisemartin Cécile , Chanez Brice , Meillat Hélène , Lelong Bernard , Poizat Flora , Caillol Fabrice , Giovannini Marc 
Published: September 2023

Background and Aims: Colorectal lesions measuring greater than 20 mm are unsuitable for en bloc endoscopic mucosal resection (EMR): piecemeal EMR (PM-EMR) and endoscopic submucosal dissection (ESD) are needed. The European Society of Gastrointestinal Endoscopy (ESGE) recommends ESD only for microinfiltrative lesions, although Japanese teams perform en bloc ESD for all lesions. We report the outcomes obtained in our endoscopy unit for these lesions and assess the hybrid “knife-assisted piecemeal EMR” (KAPM-EMR) technique. The main aim was to assess the short-term outcomes (C1). The secondary objectives were to evaluate the long-term results (C2), adverse event rate and management of recurrence.
Methods: We retrospectively analyzed data from patients treated by PM-EMR, KAPM-EMR and ESD for a colorectal lesion measuring greater than 20 millimeters using prospective inclusion over four years.
Results: Data from 167 patients (median age: 70) with a median follow-up of 15.1 months were analyzed after excluding 95 patients. A total of 131 lesions were removed by PM-EMR, 24 by KAPM-EMR and 12 by ESD; 146/167 (87.4%) patients were considered in remission at C1. Recurrence was treated by endoscopy in 20/21 patients (95%); 86/89 (96.6%) were in remission at C2. A total of 16/167 patients developed adverse events, all of whom except one were endoscopically managed. KAPM-EMR was associated with a higher perforation risk (p=0.037). No differences in postoperative bleeding were found among the three groups (p=0.576).
Conclusions: Piecemeal resection remains an effective and safe technique for large colorectal adenomas. KAPM-EMR may be useful but should be applied with caution due to the risk of perforation.
Efficacy of Sacral Nerve Stimulation in Non-constipated Irritable Bowel Syndrome Patients: A Systematic Review
Authors: Garcia-Cabrera Ana Maria , De la Portilla Fernando , Jiménez-Rodríguez Rosa María , Vázquez –Monchul Jorge Manuel , Reyes- Díaz María Luisa , Ramallo -Solís Irene María , Pintor-Tortolero José , Dios-Barbeito Sandra , Padillo- Ruiz Francisco Javier 
Published: September 2023

Background and Aims: Irritable bowel syndrome (IBS) is a prevalent disorder with a complex and heterogeneous physiopathology, including a dysregulation of gut-brain axis. Treatment for IBS is targeted to the predominant symptom and requires a multidisciplinary approach. This review aims to evaluate the efficacy and safety of sacral nerve stimulation in non-constipated IBS patients
Methods: A literature search was carried out on MEDLINE, The Cochrane Central Register of Controlled Trials (CENTRAL) and Web of Science databases for all relevant articles. Quality of included papers was assessed using standardized guidelines
Results: Of 129 initial citations, 7 articles met our predefined inclusion criteria, including five randomized trials, a pilot study and a descriptive follow-up study. Five of 7 studies reported a positive effect of sacral nerve stimulation on symptoms and quality of life improvement in non-constipated IBS patients. No study reported serious adverse events.
Conclusions: Despite initial promising results of sacral nerve stimulation in non-constipated IBS patients, studies with larger sample sizes and longer follow-up are required.