bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation in hearing-related genes in non-syndromic sensorineural hearing loss patients
Authors: Eğilmez Oğuz Kadir , Can Sermet , Kalcıoğlu M. Tayyar , Tan Berna Demircan , Yılmaz Sarenur , Yiğit Özgür , Durna Muhammed Yusuf , Akdeniz Esra , Akalin Ibrahim 
Published: December 2023

Abstract
Background
Our understanding of epigenetic modifications in the inner ear is very limited. Although epigenetic regulation of genes related to individual organ- and system-limited pathologies are generally expected to be tissue-specific, DNA methylation patterns in peripheral blood (PB) are found to be associated with the presence of several diseases with no typical hematological involvement. Here, we aimed to investigate whether there is a correlation between hearing-related genes’ promoter region methylation in the PB samples with the presence of non-syndromic sensorineural hearing loss (NSSHL) with an aim of future utilization of DNA methylation as biomarkers in hearing loss. The study included 26 patients with NSSHL and a control group of 20 healthy individuals. CpG islands in the promoter regions of the GJB-2, GJB-6, and SLC24A genes were analyzed using bisulfite sequencing, and methylation percentages were analyzed.

Results
Methylation levels at the 1st region of GJB-6 and the 1st and the 4th regions of SLC26A4 were found to differ significantly (p = 0.039, p = 0.042, and p = 0.029, respectively) between the patients and the control group. There was no statistically significant difference in methylation percentages of GJB-2 promoters. We also found that parents’ consanguinity determines the methylation levels in patients’ families.

Conclusions
According to our knowledge, this is the first study that investigates epigenetic changes in the PB of patients with NSSHL. Despite the small sample size, our findings indicate that DNA methylation patterns in the PB could be of use for understanding epigenetic changes in the inner ear and the clinical management of NSSHL.
HLA Haplotypes and Relapse After Hematopoietic Cell Transplantation
Authors: Petersdorf Effie W. , McKallor Caroline , Malkki Mari , He Meilun , Spellman Stephen R. , Gooley Theodore , Stevenson Philip 
Published: December 2023

PURPOSE Recurrence of blood malignancy is the major cause of hematopoietic cell transplant failure. HLA class II molecules play a fundamental role in antitumor responses but the role of class II haplotypes is not known. METHODS HLA-DR, -DQ, -DM, and -DO allele variation was determined in 1,629 related haploidentical transplants to study the clinical significance of individual molecules and haplotypes. RESULTS Outcome correlated with patient and donor variation for HLA-DRβ residue 86 (Gly/Val), HLA-DQ (G1/G2) heterodimers, and donor HLA-DM (DM11,11/nonDM11,11) molecules, and depended on patient-donor mismatching. Risks of relapse were lower for DRβ-86 GlyGly patients when the donor was GlyVal (hazard ratio [HR], 0.46 [95% CI, 0.30 to 0.68]; P < .001); GlyVal patients benefited from HLA-DRB1–matched donors, whereas no donor was superior to another for ValVal patients. G1G2 patients with G1G2-mismatched donors had lower relapse. Transplantation from donors with DMα residue 184 ArgHis was associated with higher risk of relapse (HR, 1.60 [95% CI, 1.09 to 2.36]; P = .02) relative to ArgArg. Relapse and mortality risks differed across HLA-DR-DQ-DM haplotypes. CONCLUSION HLA class II haplotypes may be functional constituents of the transplantation barrier, and their consideration in patients and donors may improve the success of transplantation.
A Population Model of Time-Dependent Changes in Serum Creatinine in (Near)term Neonates with Hypoxic-Ischemic Encephalopathy During and After Therapeutic Hypothermia
Authors: Krzyzanski Wojciech , Wintermark Pia , Annaert Pieter , Groenendaal Floris , Şahin Suzan , Öncel Mehmet Yekta , Armangil Didem , Koc Esin , Battin Malcolm R. , Gunn Alistair J. , Frymoyer Adam , Chock Valerie Y.-L. , Keles Elif , Mekahli Djalila , van den Anker John , Smits Anne , Allegaert Karel 
Published: December 2023

AbstractThe objective was to apply a population model to describe the time course and variability of serum creatinine (sCr) in (near)term neonates with moderate to severe encephalopathy during and after therapeutic hypothermia (TH). The data consisted of sCr observations up to 10 days of postnatal age in neonates who underwent TH during the first 3 days after birth. Available covariates were birth weight (BWT), gestational age (GA), survival, and acute kidney injury (AKI). A previously published population model of sCr kinetics in neonates served as the base model. This model predicted not only sCr but also the glomerular filtration rate normalized by its value at birth (GFR/GFR0). The model was used to compare the TH neonates with a reference full term non-asphyxiated population of neonates. The estimates of the model parameters had good precision and showed high between subject variability. AKI influenced most of the estimated parameters denoting a strong impact on sCr kinetics and GFR. BWT and GA were not significant covariates. TH transiently increased $$\mathrm{sCr}$$
sCr
in TH neonates over the first days compared to the reference group. Asphyxia impacted not only GFR, but also the $$\mathrm{sCr}$$
sCr
synthesis rate. We also observed that AKI neonates exhibit a delayed onset of postnatal GFR increase and have a higher $$\mathrm{sCr}$$
sCr
synthesis rate compared to no-AKI patients. Our findings show that the use of $$\mathrm{sCr}$$
sCr
as marker of renal function in asphyxiated neonates treated with TH to guide dose selection for renally cleared drugs is challenging, while we captured the postnatal sCr patterns in this specific population.
Graphical Abstract
Investigating a method for macroscale energy extraction from random electrons movement
Authors: Peivandi M. , Kouhikamali R. 
Published: December 2023

Abstract
This paper aims to investigate a novel method for regularizing the random movements of electrons, in order to generate net movement and extract energy from the resulting electromotive force. The proposed method involves designing anisotropic conditions, focusing on exploring the effects of surface roughness on a metal wire in a two-dimensional space with the presence of a magnetic field. This paper presents a mathematical model for studying the proposed method. Furthermore, simulation results are analyzed and discussed in the context of the proposed method. By investigating the effects of surface roughness on electrons movement and voltage generation, this paper aims to contribute to the development of new methods for energy extraction from random electrons movement.
Melanin-Concentrating Hormone Is Associated With Delayed Neurocognitive Recovery in Older Adult Patients With Preoperative Sleep Disorders Undergoing Spinal Anesthesia
Authors: Gao Yi , Zhou Qi , Li Haibo , Zhao Yunjiao , Zhu Hongyan , Zhang Xizhe , Li Yun 
Published: December 2023

BACKGROUND:
Aging and preoperative sleep disorders are the main risk factors affecting postoperative cognitive outcomes. However, the pathogenesis of delayed neurocognitive recovery after surgery remains ambiguous, and there is still a lack of potential biomarkers for delayed neurocognitive recovery in older adult patients with preoperative sleep disorders. Our study aimed to explore the relationship between melanin-concentrating hormone (MCH) and delayed neurocognitive recovery early after surgery in older adult patients with preoperative sleep disorders.

METHODS:
In this monocentric prospective observational study, 156 older adult patients (aged 65 years or older) with preoperative sleep disorders undergoing elective total hip arthroplasty (THA) or total knee arthroplasty (TKA) were included at an academic medical center in Inner Mongolia, China, from October 2021 to November 2022, and all patients underwent spinal anesthesia. The Pittsburgh Sleep Quality Index (PSQI) was applied to assess the preoperative sleep quality of all patients, and preoperative sleep disorders were defined as a score of PSQI >5. We measured the levels of cerebrospinal fluid (CSF) MCH and plasma MCH of all patients. The primary outcome was delayed neurocognitive recovery early after surgery. All patients received cognitive function assessment through the Montreal Cognitive Assessment (MoCA) 1 day before and 7 days after surgery (postoperative day 7 [POD7]). Delayed neurocognitive recovery was defined as a score of POD7 MoCA <26. The potential confounders included variables with P < .2 in the univariate logistic analysis, as well as the important risk factors of delayed neurocognitive recovery reported in the literature. Multivariable logistic regression model based on the Enter method assessed the association of MCH and delayed neurocognitive recovery in older adult patients with preoperative sleep disorders.

RESULTS:
Fifty-nine (37.8%) older adult patients with preoperative sleep disorders experienced delayed neurocognitive recovery at POD7. Increase in CSF MCH levels (odds ratio [OR] for an increase of 1 pg/mL = 1.16, 95% confidence interval [CI], 1.09–1.23, P < .001) and decrease in plasma MCH levels (OR for an increase of 1 pg/mL = 0.92, 95% CI, 0.86–0.98, P = .003) were associated with delayed neurocognitive recovery, after adjusting for age, sex, education, baseline MoCA scores, American Society of Anesthesiologists (ASA) grade, and coronary heart disease (CHD).

CONCLUSIONS:
In older adult patients with preoperative sleep disorders, MCH is associated with the occurrence of delayed neurocognitive recovery after surgery. Preoperative testing of CSF MCH or plasma MCH may increase the likelihood of identifying the high-risk population for delayed neurocognitive recovery in older adult patients with preoperative sleep disorders.
Impact of Multiple Sclerosis Subtypes on Pain Management in Patients With Trigeminal Neuralgia After Stereotactic Radiosurgery: An International Multicenter Analysis
Authors: De Nigris Vasconcellos Fernando , Mashiach Elad , Alzate Juan Diego , Bernstein Kenneth , Rotman Lauren , Levy Sarah , Qu Tanxia , Wegner Rodney E. , Shepard Matthew J. , Patel Samir , Warnick Ronald E. , Moreno Nuria Martínez , Martínez Álvarez Roberto , Picozzi Piero , Franzini Andrea , Peker Selçuk , Samanci Yavuz , Elguindy Ahmed N. , Palmer Joshua D. , Lunsford L. Dade , Jose Shalini G. , Wei Zhishuo , Niranjan Ajay , Blagui Sarra , Iorio-Morin Christian , Mathieu David , Briggs Robert G , Yu Cheng , Zada Gabriel , Dayawansa Samantha , Sheehan Jason , Schulder Michael , Goenka Anuj , Begley Sabrina , Khilji Hamza , Urgošík Dušan , Liščák Roman , Kondziolka Douglas 
Published: December 2023

BACKGROUND AND OBJECTIVES:
Trigeminal neuralgia affects approximately 2% of patients with multiple sclerosis (MS) and often shows higher rates of pain recurrence after treatment. Previous studies on the effectiveness of stereotactic radiosurgery (SRS) for trigeminal neuralgia did not consider the different MS subtypes, including remitting relapsing (RRMS), primary progressive (PPMS), and secondary progressive (SPMS). Our objective was to investigate how MS subtypes are related to pain control (PC) rates after SRS.

METHODS:
We conducted a retrospective multicenter analysis of prospectively collected databases. Pain status was assessed using the Barrow National Institute Pain Intensity Scales. Time to recurrence was estimated through the Kaplan-Meier method and compared groups using log-rank tests. Logistic regression was used to calculate the odds ratio (OR).

RESULTS:
Two hundred and fifty-eight patients, 135 (52.4%) RRMS, 30 (11.6%) PPMS, and 93 (36%) SPMS, were included from 14 institutions. In total, 84.6% of patients achieved initial pain relief, with a median time of 1 month; 78.7% had some degree of pain recurrence with a median time of 10.2 months for RRMS, 8 months for PPMS, 8.1 months for SPMS (P = .424). Achieving Barrow National Institute-I after SRS was a predictor for longer periods without recurrence (P = .028). Analyzing PC at the last available follow-up and comparing with RRMS, PPMS was less likely to have PC (OR = 0.389; 95% CI 0.153-0.986; P = .047) and SPMS was more likely (OR = 2.0; 95% CI 0.967-4.136; P = .062). A subgroup of 149 patients did not have other procedures apart from SRS. The median times to recurrence in this group were 11.1, 9.8, and 19.6 months for RRMS, PPMS, and SPMS, respectively (log-rank, P = .045).

CONCLUSION:
This study is the first to investigate the relationship between MS subtypes and PC after SRS, and our results provide preliminary evidence that subtypes may influence pain outcomes, with PPMS posing the greatest challenge to pain management.
Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy
Authors: Riddell Dominique O. , Hildyard John C. W. , Harron Rachel C. M. , Taylor-Brown Frances , Kornegay Joe N. , Wells Dominic J. , Piercy Richard J. 
Published: December 2023

ABSTRACT
Duchenne muscular dystrophy (DMD), caused by mutations in the dystrophin (DMD) gene, is associated with fatal muscle degeneration and atrophy. Patients with DMD have progressive reductions in skeletal muscle strength and resistance to eccentric muscle stretch. Using the DE50-MD dog model of DMD, we assessed tibiotarsal joint (TTJ) flexor and extensor force dynamics, and the resistance of dystrophic muscle to eccentric stretch. Male DE50-MD and wild-type (WT) dogs were analysed every 3 months until 18 months of age. There was an age-associated decline in eccentric contraction resistance in DE50-MD TTJ flexors that discriminated, with high statistical power, WT from DE50-MD individuals. For isometric contraction, at the majority of timepoints, DE50-MD dogs had lower maximum absolute and relative TTJ flexor force, reduced TTJ muscle contraction times and prolonged relaxation compared to those in WT dogs. Cranial tibial muscles, the primary TTJ flexor, of 18-month-old DE50-MD dogs had significant numbers of regenerating fibres as expected, but also fewer type I fibres and more hybrid fibres than those in WT dogs. We conclude that these parameters, in particular, the eccentric contraction decrement, could be used as objective outcome measures for pre-clinical assessment in DE50-MD dogs.
Harnessing Pakistan’s youth for tobacco control
Authors: Saeed Saima , Zeeshan Kinza , Khan Fizra 
Published: December 2023

doi: https://doi.org/10.12669/pjms.40.2(ICON).8953
How to cite this: Saeed S, Zeeshan K, Khan F. Harnessing Pakistan’s youth for tobacco control. Pak J Med Sci. 2024;40(2):S107-S108. doi: https://doi.org/10.12669/pjms.40.2(ICON).8953
Sosialisasi Earth Hour Sebagai Upaya Gerakan Penghematan Listrik Melalui Siaran Radio Suara Tebuireng Jombang
Authors: Izzati Nailul , Wardana Humaidillah Kurniadi , Leksono Jati Widyo 
Published: December 2023

Indonesia merupakan salah satu negara dengan penduduk paling banyak di dunia. Memiliki 275,5 juta jiwa, Indonesia berada di urutan keempat, negara dengan jumlah penduduk terbanyak. Semakin banyak jumlah penduduk, semakin banyak juga kebutuhan terhadap sumber daya. Di sisi lain, sumber daya alam berbasis fosil setiap harinya semakin menipis. Sumber daya yang dimaksud adalah yang terkait dengan ketersediaan listrik. Mengingat listrik adalah salah satu kebutuhan hidup yang utama, maka diperlukan upaya untuk mengatasinya. Salah satu cara yang dilakukan oleh para ahli adalah dengan mengeksplor sumber daya alternatif. Sedangkan bagi masyarakat, dapat dilakukan dengan cara penghematan. Ada beberapa gerakan penghematan listrik, diantaranya adalah Earth Hour. Sosialisasi Earth Hour. dilakukan agar masyarakat mengerti pentingnya penghematan listrik, meningkatkan pemahaman serta kesadaran dan mendorong masyarakat untuk berkontribusi dalam gerakan tersebut. Melalui Siaran Radio Suara Tebuireng, tim pengabdian memaparkan pentingnya hemat dan bijak dalam menggunakan listrik. Pemaparan disajikan berupa bincang santai dan interaktif bersama penyiar radio dan para pendengar. Sosialisasi berjalan lancar, beberapa pertanyaan masuk dari pendengar. Dengan sosialisasi tersebut, pengetahuan dan wawasan yang diperoleh pendengar dapat disebarluaskan kepada masyarakat di sekitarnya, setidaknya dipraktikkan dalam keseharian di rumah masing-masing. Dengan demikian, masyarakat dapat terinspirasi dan ikut berkontribusi dalam berhemat, atau menggunakan listrik secara bijak.
VLIV PŘEDPOKLÁDANÉ HODNOTY PŘI ZADÁVÁNÍ VEŘEJNÝCH ZAKÁZEK
Authors: Háva Karla 
Published: December 2023

Veřejný zadavatel stanovuje obvykle před zahájením zadávacího řízení veřejné zakázky její předpokládanou hodnotu. Příspěvek se zabývá veřejnými zakázkami na stavební práce a zkoumá vliv předpokládané hodnoty na výsledky zadávacího řízení. Článek zjišťuje závislost výše vítězné ceny ve veřejné zakázce na výši předem stanovené předpokládané hodnoty. Dále pak zkoumá závislost rozdílu vítězné ceny a předpokládané hodnoty na výši předpokládané hodnoty a v poslední řadě pak závislost počtu nabídek ve veřejné zakázce na výši předpokládané hodnoty. Za účelem vyhodnocení výzkumu byl prošetřen výzkumný vzorek 165 veřejných zakázek. K vyhodnocení jsou použity statistické metody, konkrétně korelační a regresní analýza.
Bylo zjištěno, že vítězná cena veřejné zakázky na stavební práce silně závisí na výši její předpokládané hodnoty. Dále bylo zjištěno, že rozdíl vítězné ceny a předpokládané hodnoty je silně závislý na výši předem stanovené předpokládané hodnoty. Závislost počtu nabídek ve výběrovém řízení na výši předpokládané hodnoty byla zjištěna středně silná.
Influencers y etiquetado frontal en México. Aproximaciones desde la campaña #ParaVariar de Sidral Mundet
Authors: Olmedo Neri Raul Anthony , García Calderón Carola 
Published: December 2023

En los últimos años se ha observado la tendencia de la publicidad para posicionar sus marcas en internet sin acatar enteramente el marco regulatorio en México. Como estudio de caso se revisa el uso del etiquetado frontal en los contenidos de los influencers en la campaña digital #ParaVariar de Sidral Mundet. A partir del análisis iconográfico-semiótico se revisaron los contenidos producidos por cinco influencers a fin de identificar cómo se muestra el etiquetado frontal y cuál es su función dentro de la narrativa publicitaria. Los resultados muestran que dichos contenidos tienden a ocultar el etiquetado frontal, así como a no mostrar explícitamente que se trata de una colaboración pagada.
The efficacy of audiovisual distraction as an anxiety-minimizing technique during echocardiography in preschool children
Authors: Yantie Ni Putu Veny Kartika , Gunawijaya Eka , Windiani I Gusti Ayu Trisna , Maharini Kadek 
Published: December 2023

Background Echocardiography procedures can cause fear and anxiety in children, especially at preschool age. Audiovisual distraction is a simple, harmless, and low-cost technique that does not interfere with the procedure.
Objective To assess the effect of audiovisual distraction on the anxiety levels of children aged 2-5 years during echocardiography.
Methods This quasi-experimental study included children aged 2-5 years who were admitted for echocardiography. Patients with hearing or visual impairment, Down syndrome, autism, or attention-deficit/hyperactivity disorder were excluded. We administered anxiety-reducing intervention in the form of cartoon audiovisual media shown on a ceiling-mounted television during the echocardiography procedure. Anxiety levels were assessed using the Visual Analogue Scale - Anxiety (VAS-A) before and after the intervention was given. We compared the subjects’ pre- and post-procedure VAS-A scores and heart rates.
Results Of the 43 subjects who underwent echocardiography during the study period, the mean age was 3.2 (SD 0.9) years, with an equal sex distribution. Most subjects had a history of repeated echocardiography procedures and hospitalizations. Thirty-nine (90.6%) of subjects demonstrated anxiety during the echocardiography procedure. There was a significant mean difference of 11.9 (SD 13.2) bpm (95%CI 7.8 to 15.9 bpm, p<0.001) in heart rate pre- vs. post-intervention. Median pre- and post-intervention VAS-A scores were 5 (range 0-10) and 1 (range 0-4), respectively (P=0.001). Post-intervention, almost all (97.7%) subjects had no or mild anxiety.
Conclusion Audiovisual content presented on television serves as an effective distraction method to reduce children's anxiety during echocardiography.
PRÁTICAS RESTAURATIVAS
Authors: Silva de Andrade Costa Duvernoy Doriele , Lopes de Souza Gildo 
Published: December 2023

A violência é um fenômeno social complexo e de múltiplas causalidades. Nesta pesquisa, objetivamos analisar em que medida o projeto de intervenção “Práticas Restaurativas na Escola” contribuiu para reflexão e planejamento de Círculos de Construção de Paz na escola. Trata-se de uma pesquisa-ação, a partir de questionário semiestruturado, grupo focal e oficinas. Na etapa diagnóstica, 09 gestores e 73 professores de escolas da rede de ensino da cidade de Aliança-PE nos forneceram indicações sobre as violências presentes na escola. Verificamos desconhecimento sobre práticas restaurativas, terceirização da responsabilidade no enfrentamento da violência e ações punitivistas na resolutividade: suspensão, transferências, punição. A partir disso, o grupo focal, composto por 08 integrantes, participou de oficinas sobre Práticas Restaurativas e construiu coletivamente Círculos de Construção de Paz. Com isso, os participantes da pesquisa foram sensibilizados a desenvolver ações que contribuíssem para o desenvolvimento do clima organizacional da escola, através das práticas restaurativas, lançando novos olhares sobre as situações de violência presentes na escola. Desta forma, os Círculos de Construção de Paz agregam princípios e práticas cotidianas que promovem o diálogo, fortalecem o sentimento de pertencimento, capacidade empática e construção de um ambiente seguro, inclusivo e de vivência de processos educativos.
A COORDENAÇÃO PEDAGÓGICA COMO ARTICULADORA NA IMPLEMENTAÇÃO DE POLÍTICAS EDUCACIONAIS NA ESCOLA
Authors: De Castro Pitano Sandro , Zilli Polesello Julsemina 
Published: December 2023

O artigo aborda o papel da coordenação pedagógica na implementação de políticas educacionais na escola. Por meio de entrevista coletiva, analisa a atuação das coordenadoras junto à rede municipal de educação de Nova Prata (RS), visando compreender como se processa a articulação com os professores, os desafios, os limites e as possibilidades para a implementação da Pedagogia de Projetos. Na fundamentação teórica, amparou-se em autores como Gallon, Rocha Filho e Machado (2017), Celestino (2013), Araújo e Ribeiro (2016) e Araújo, Martins e Rodrigues (2019), com ênfase no papel da coordenação pedagógica na escola, dialogando, também, com reflexões de Nóvoa (2002), Saviani (2006), Libâneo (2004) e Pimenta (1997). Constatou-se a dificuldade de atuação interdisciplinar por parte dos professores, a necessidade de planejamento coletivo e de espaços e tempos destinados à formação como subsídio ao papel da coordenação. A função articuladora, assumida como hipótese de trabalho, confirmou-se ao longo da pesquisa, afirmando a coordenação como uma referência para a prática pedagógica dos professores diante dos desafios emergentes no cotidiano escolar.