bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of local delivery of PDGF‐BB on attachment of human periodontal ligament fibroblasts to periodontitis‐affected root surfaces – in vitro
Authors: Gamal Ahmed Y. , Mailhot Jason M. 
Published: March 2003

AbstractBackground/aims: The purpose of this study was to determine at what concentration does platelet‐derived growth factor‐BB (PDGF‐BB) provide for optimal stimulation of human periodontal ligament fibroblasts (PDL) to adhere to periodontitis affected root surfaces.Method: 80 root dentine specimens were prepared from extracted periodontally diseased teeth obtained from patients ranging in age between 35 to 60 years. The root dentine specimens were associated with the subgingival area opposing the periodontal pocket for each extracted tooth. 10 healthy root dentine specimens were obtained from teeth extracted for orthodontic reasons and served as controls. The specimens were distributed into 9 groups (10 specimens in each). In group 1, PDL fibroblasts were cultured on the specimen surface of a diseased treated control. In group 2, PDL fibroblasts were cultured on the specimen surface of a healthy control. In groups 3 to 9, PDL fibroblasts were cultured on a pretreated specimen surface with concentrations ranging from 5, 10, 20, 50, 100, 200 and 300 ng/ml PDGF‐BB, respectively. After 24 h incubation, the media were removed, specimens were fixed, processed for SEM viewing and photographed at 750 ×. Fibroblast adherence was measured by counting number of cells within a standard test area and cell morphology was scored.Results: Findings suggest dentine specimens pretreated with 5, 10 and 20 ng/ml PDGF‐BB were not significantly different in number of adherent cells from the diseased treated control. However, at concentrations of 50, 100, 200 and 300ng/ml, a highly significant increase in number of adherent fibroblasts was detected when compared to the diseased treated control. At these concentrations, the cell morphology was comparable to that of the healthy control.Conclusions: PDGF‐BB in concentrations equal to or greater than 50 ng/ml demonstrates a significant stimulation of PDL cells adherence to periodontal diseased root surfaces. Since the higher concentrations resulted in similar effects as obtained by 50 ng/ml, it may therefore be considered that this concentration provides for optimal stimulation of human periodontal ligament fibroblasts (PDL) to adhere to periodontitis‐affected root surfaces.
Recombinant human bone morphogenetic protein‐7 in maxillary sinus floor elevation surgery in 3 patients compared to autogenous bone grafts
Authors: Van Den Bergh J. P. A. , Ten Bruggenkate C. M. , Groeneveld H. H. J. , Burger E. H. , Tuinzing D. B. 
Published: March 2003

AbstractBackground/Aims: This pilot study was designed to determine the clinical bone formation ability of a human recombinant DNA bone morphogenetic protein‐7, also referred to as Osteogenic Protein‐1 [OP‐1] combined with a collagen carrier, implanted in the maxillary sinus of 3 patients. The results were compared with a group of 3 patients treated with sinus floor elevation and autogenous bonegrafts.Methods: 6 consecutive patients, 4 female and 2 male, between 48 and 57 years of age were treated by means of sinus floor elevation for insufficient bone height in the posterior maxilla for implant surgery. 3 patients, 2 female and 1 male, were treated with OP‐1 attached to a collagen device. In these patients, 4 maxillary sinus grafting procedures according to Tatum's method were carried out. 1 g of collagen carrier containing 2.5 mg rhOP‐1 mixed with 3 ml of saline was placed between the bony floor and the elevated mucosal lining of the most caudal part of the maxillary sinus, in order to increase the vertical bone dimension to place dental implants of a sufficient length. The 3 other patients, also 2 female and 1 male, with a total of 5 sinus sites, were treated with sinus floor elevation and autogenous iliac crest bonegrafts. After 6 months, during dental implant preparation, bone cores were taken for histology. Thus, clinical, radiological and histological results of the 2 groups of 3 patients were compared.Results: 6 months after sinus grafting with OP‐1, in 1 male, well‐vascularized bonelike tissue of good quality was observed clinically. This could be confirmed by histology. In the second, female, patient no bone formation was observed at all. A cyst‐like granular tissue mass, without purulent content, was removed. In the 3rd, female, patient, who received bilateral sinus grafts, some bonelike formation was seen, however it showed flexible tissue which led to the decision that at 6 months after the sinus grafting, the implant placement had to be postponed. In all 5 autogenous grafted sinuses a bone appearance similar to normal maxillary bone was observed clinically as well as histologically and dental implants could be placed six months after sinus floor elevation surgery.Conclusions: These findings indicate that the OP‐1 device has the potential for initiating bone formation in the human maxillary sinus within 6 months aftera sinus floor elevation operation. However, the various findings in these 3 patients indicate that the behaviour of the material is at this moment insufficiently predictable, in this indication area. Further investigation is indicated before OP‐1 can be successfully used instead of the “gold standard” autogenous bone graft.
Classifications of oral lesions in HIV infection
Authors: Narani Nazanin , B. Epstein Joel 
Published: March 2003

AbstractBackground: Manifestations of immunosuppression may take the form of opportunistic infection, and neoplasia. While this paper has focused on gingival and periodontal manifestations, these tissues cannot be evaluated in isolation. The presence of involvement of other oral tissues such as the cheek or tongue with manifestations associated with HIV such as hairy leukoplakia, Kaposi’s sarcoma at these sites, and candidiasis in addition to periodontal manifestations may further increase the clincial suspicion of underlying immunosuppression and/or progression of the immunosuppressive state.Discussion: The periodontist plays an essential rôle in identifying the periodontal status of an individual and has an important rôle to play in early recognition of signs and symptoms of HIV disease or progression of the medical condition.Conclusion: Only through such recognition can appropriate definitive diagnostic testing be conducted, and appropriate therapeutic intervention for the oral condition and the systemic condition be considered.
Periodontal status and serum antibody titers for Porphyromonas gingivalis fimbriae in a rural population in Japan
Authors: Furuichi Y. , Ito H.‐O. , Izumi Y. , Matsuyama T. , Yotsumoto Y. , Mishige Y. , Kojima M. , Yamashita K. , Inoue M. 
Published: March 2003

AbstractBackground, aims: The present study was undertaken to assess the periodontal status of a rural Japanese population and to study the correlation between the periodontal status and the serum antibody titers for Porphyromonas gingivalis (Pg) fimbriae.Method: A total of 236 individuals were examined for their periodontal conditions by the use of the community periodontal index for treatment needs (CPITN), and serum antibody titers for Pg fimbriae in their peripheral blood samples were evaluated using the enzyme‐linked immunosorbent assay.Results: There was a substantially larger proportion of edentulous subjects in the age group older than 60 years. The remaining teeth were 24.1, 23.2, 11.1 and 10.1 per person in the 40–49, 50–59, 60–69 and 70 age groups, respectively. The % of sextants with a CPITN code of missing sextant (MS) increased towards elderly and reached >60% in the age group of 70 years, as the % of the CPITN 2, 1 or 0 sextant decreased. The % of CPITN 4 and 3 sextants did not differ between different age groups and were about 6–8% and 15–20%, respectively. The % of CPITN 1 or 0 sextants was higher in female subjects than in male subjects in the 60–69 and 70 age groups, while the % of CPITN 4 or 3 sextants was higher in male subjects than in female subjects in all age groups. There was no significant difference between various age groups in the mean serum antibody titers for Pg fimbriae. The mean anti‐Pg fimbriae antibody titers was significantly higher for the subjects with a maximum CPITN code 4 (max.‐CPITN 4 subject) than for the subjects with lower maximum CPITN codes. The antibody titers varied extensively among the max.‐CPITN 4 or 3 subjects, but not among the max.‐CPITN 2/1/0 or MS subjects.Conclusions: The present study demonstrated that tooth loss is a remarkable event in elderly subjects and that oral prophylaxis and mechanical debridement should be mandatory in the population examined. It was also demonstrated that the serum antibody titers against Pg fimbriae could be useful for screening individuals with moderate to severe periodontitis.
Concentration of interleukin‐1β and neutrophil elastase activity in gingival crevicular fluid during experimental gingivitis
Authors: Gonzáles J. R. , Herrmann J. M. , Boedeker R. H. , Francz P. I. , Biesalski H. , Meyle J. 
Published: March 2003

AbstractBackground, aim: The aim of the present study was to measure interleukin‐1β concentrations and neutrophil elastase activity in gingival crevicular fluid (GCF) during experimental gingivitis in humans.Material and Methods: 12 healthy young men participated. After prophylaxis, they performed optimal hygiene to reach plaque and gingivitis indices of or approaching zero. All oral hygiene measures were then ceased for a period of 18 days. The Quigley‐Hein plaque index (PLI) and Saxer & Mühlemann papillary bleeding index (PBI) were assessed. GCF samples were taken from the mesiobuccal site of two contralateral teeth in the upper jaw by means of periopapers at baseline and on days 3, 7, 14 and 18. After measuring the gingival crevicular fluid volume (GCFV) with the Periotron 8000, the samples were analyzed in our laboratory for the detection of IL‐1β concentration by ELISA.Results: PLI and PBI showed a reduction prior to baseline reaching almost zero, both increasing from day 0 to day 18 (PLI=from 0.1 to 2.9, PBI=from 0 to 2.0). IL‐1β concentration increased from 229.25 ng/ml (day 0) to 526.13 ng/ml (day 18). Clinical data and IL‐1β concentrations were correlated with elastase activity (EA). No significant correlation could be demonstrated between the clinical parameters assessed and IL‐1β or EA (Spearman rank correlation coefficient). A correlation between GCFV and PBI from day 0 to day 18 could be demonstrated.Conclusion: Overall, both IL‐1β and EA showed an increase from baseline throughout the whole study.
Evaluation of the effect of tooth vitality on regenerative outcomes in infrabony defects
Authors: Cortellini Pierpaolo , Tonetti Maurizio S. 
Published: March 2003

AbstractBackground, aims: This investigation was designed to evaluate the null hypothesis of no differences in GTR outcomes in intrabony defects at vital and successfully root‐canal‐treated teeth.Method: 208 consecutive patients with one intrabony defect each were enrolled. Based on tooth vitality, the treated population was divided at baseline into 2 groups: one with 41 non‐vital teeth and the other with 167 vital teeth. The 2 groups were similar in terms of patient and defect characteristics.Results: A slight unbalance in terms of depth of the intrabony component was observed in the non‐vital group compared to the vital group (6.9±2.1 mm versus 6.2±2.3 mm, p=0.08). All defects were treated with GTR therapy. At 1 year, the non‐vital and the vital groups showed a clinical attachment level (CAL) gain of 4.9±2.2 mm and of 4.2±2 mm, respectively. The difference was statistically significant (p=0.03). To correct for the baseline unbalance in defect depth, data were expressed as a % of clinical attachment level gains with respect to the original intrabony depth of the defect. % CAL gains were 72.8±42.2% and 73±26.4% for vital and non‐vital teeth, respectively: the difference was not statistically significant (p=0.48). Average residual pocket depths were 2.8±1 mm in the vital and 2.8±0.9 mm in the non‐vital group. Tooth vitality was assessed at baseline, at 1‐year and at follow‐up (5.4±2.8 years after surgery): all teeth vital at baseline were still vital at follow‐up with the exception of 2 teeth that received endodontic treatment for reconstructive reasons and for caries. At follow‐up visit, the difference in CAL with respect to 1‐year measurements was −0.9±0.8 mm in the vital group and −0.7±0.8 mm in the non‐vital group, indicating stability of the regenerated attachment at the majority of sites.Conclusions: Data from this study demonstrate that root canal treatment does not negatively affect the healing response of deep intrabony defects treated with GTR therapy; furthermore GTR therapy in deep intrabony defects does not negatively influence tooth vitality.
Tacrolimus is not associated with gingival overgrowth in renal transplant patients
Authors: James Jacqueline A. , Jamal Sundos , Hull Peter S. , Macfarlane Tatiana V. , Campbell Babatunde A. , Johnson Robert W. G. , Short Colin D. 
Published: March 2003

AbstractBackground: Cyclosporin A is used extensively to prevent the rejection of allogenic renal transplants. However, it is associated with a variety of undesirable side effects including gingival overgrowth. Tacrolimus (FK506), has been marketed as an effective alternative immunosuppressant to cyclosporin A and recent subjective reports suggest patients taking it complain infrequently of gingival problems. This clinical investigation was undertaken to confirm whether or not tacrolimus adversely affected the gingival health of renal transplant recipients.Methods: Renal transplant patients (RTPs) under the care of the Renal Transplantation Service at the Manchester Royal Infirmary, who had received a renal allograft at least 18 months earlier, were recruited for this study. All but one of the RTPs had been taking tacrolimus since transplantation. The other had commenced tacrolimus therapy two months after receiving her allograft. A hospital based control group was recruited from non transplanted individuals attending the Turner Dental School, Manchester. Each patient underwent a detailed dental assessment and had dental impressions taken. The extent of gingival overgrowth was determined from plaster models.Results: 25 renal transplant recipients and 26 control patients were included in the study. None of the individuals in either the tacrolimus or control groups had clinically significant overgrowth. The patients in the tacrolimus group with the highest overgrowth scores were those also taking calcium antagonists as treatment for hypertension.Conclusion: This study demonstrates that tacrolimus has no adverse effects on the gingival tissues and thus has potential as an alternative immunosuppressant for individuals susceptible to developing cyclosporin A‐induced gingival overgrowth.
The gingival inflammatory infiltrate in cardiac patients treated with calcium antagonists*
Authors: Bullón Pedro , Machuca Guillermo , Armas José R. , Rojas José L. , Jiménez Gema 
Published: March 2003

AbstractObjectives: To analyse the periodontal inflammatory infiltrates in patients with cardiac disease, some of these patients were treated with calcium antagonists (nifedipine and diltiazem) and some were not, to compare them with a healthy control group, and to evaluate the changes in the inflammatory infiltrate after periodontal treatment.Material and methods: A “healthy group” (HG, n=12), a “cardiac group” (CG, n=12) without treatment with calcium antagonists, a “nifedipine group” (NG, n=18) and a “diltiazem group” (DG, n=13) were analysed. Biopsies were taken from a zone 2–3 mm below the upper part of the interproximal papillae 12–13 and 33–32 before causal periodontal treatment and after 1 year. Using haematoxylin‐eosin staining, the plasma cells (P), lymphocytes (L), histiocytes (H) and polymorphonuclear cells (PMN) were counted. T and B lymphocytes were evaluated using the monoclonal antibodies anti‐CD20 and anti‐CD45RO. Statistical tests used: χ2 for study of the sample composition; ANOVA for comparison between groups; Student t‐test and Wilcoxon test for comparison between visits; post‐hoc test Bonferroni.Results: When the cells were compared statistically, differences were stablished for L at the first visit (p<0.00001) and at the last visit (p<0.02), for the B lymphocytes (first visit p<0.0021, last visit p<0.022) and for the T lymphocytes (first visit p<0.0042, last visit p<0.0021). Between the 2 visits, HG showed significant reductions for P (p<0.01), L (p<0.045) and H (p<0.033); and the NG for L (p<0.0001). Lymphocytes showed differences in the NG with respect to the B lymphocytes (p<0.008).Conclusions: Nifedipine affects the inflammatory infiltrate with a greater number of lymphocytes (especially B) and these cells fell significantly in number after periodontal treatment.
Comparison of ready‐to‐use EMDOGAIN®‐gel and EMDOGAIN® in patients with chronic adult periodontitis
Authors: Bratthall G. , Lindberg P. , Havemose‐Poulsen A. , Holmstrup P. , Bay L. , Söderholm G. , Norderyd O. , Andersson B. , Rickardsson B. , Hallström H. , Kullendorff B. , Sköld Bell H. 
Published: March 2003

AbstractObjectives: The aim of this multicenter trial was to compare the clinical and radiographical outcome of a ready‐to‐use Emdogain®‐gel (test) with the marketed Emdogain® (control).Methods: Subjects with bilateral infrabony defects 4 mm deep and 2 mm wide according to radiographs were selected. 88 subjects with probing pocket depth (PPD) 6 mm 1 month after supervised oral hygiene and scaling participated. At baseline plaque index, bleeding on probing, PPD and probing attachment level were recorded and reproducible radiographs for computer‐based bone level measurements were taken. In each subject, 1 tooth was randomly treated with the test and 1 tooth with the control gel. Examinations were repeated 8 and 16 months post‐operatively.Results: After 16 months, the mean test PPD was 4.1 mm and the mean control PPD 4.2 mm. The mean gain of attachment was 2.7 mm for test and 2.9 mm for the control sites, and the radiographic measurements demonstrated a mean gain of 1 mm for both test and control sites.Conclusion: This series of cases demonstrated a statistically significant reduction of pocket depths and gain of attachment and bone after 8 and 16 months with no difference between the 2 preparations.
Application of an adjusted χ2 statistic to site‐specific data in observational dental studies
Authors: Ahn Chul , Jung Sin‐Ho , Donner Allan 
Published: March 2003

AbstractBackground: When a binary response is observed on teeth from each subject belonging to 2 or more exposure groups, application of the usual Pearson χ2 tests is invalid, since such responses within the same subject are not independent. Consequently, special statistical methods are needed to control for the correlation among teeth (sites) within the same subject. A simple adjustment to the Pearson χ2 statistic has been proposed for comparing proportions in site‐specific data. However, the required assumptions for this statistic have not yet been thoroughly addressed. These assumptions are guaranteed to hold in experimental comparisons, but may be violated in some observational studies.Method: We investigate the conditions under which the adjusted χ2 statistic is valid and examine the performance of the adjusted χ2 statistic when these conditions are violated.Results: Our simulation study shows that the adjusted χ2 statistic generally produces good empirical type I errors under the assumption of a common intracluster correlation coefficient. Even if the intracluster correlations are different, the adjusted statistic performs well when the groups have equal numbers of clusters (subjects).Conclusion: The discussion is illustrated using an observational study of caries on the roots of teeth.
A laser Doppler study of gingival blood flow variations following periosteal stimulation
Authors: Ambrosini Pascal , Cherene Sabine , Miller Neal , Weissenbach Michel , Penaud Jacques 
Published: March 2003

AbstractObjectives: Evaluation of the modifications occurring in human gingival blood flow following periosteal stimulation.Material and methods: Laser Doppler was used to measure the gingival blood flow (GBF). The reproducibility of the technique was validated by comparing measures made at intervals of 1 week. Sensitivity was verified by recording GBF before and after injection of an anesthesic containing a vasoconstrictor. Finally, 12 patients were subjected to GBF measurements before and 8 days after periosteal stimulation prior to gingival grafting.Results: The laser Doppler accurately measured GBF. Measurements made at day 0 and day 7 were not statistically different (p=0.60). After injection of an anesthesic solution containing vasoconstrictor, the laser Doppler recorded a sharp decrease of the GBF (p=0.04). The patient that underwent periosteal stimulation showed statistically significant increases (p=0.02) in GBF before and 1 week post‐stimulation.Conclusion: Periosteal stimulation induces significant increases in the GBF after 1 week.
White blood cell count in generalized aggressive periodontitis after non‐surgical therapy
Authors: Christan Claudia , Dietrich Thomas , Hägewald Stefan , Kage Andreas , Bernimoulin Jean‐Pierre 
Published: March 2003

AbstractBackground: Periodontal bacteria are known to invade the systemic circulation. Chronic low‐level bacteremia and a systemic inflammatory response have been suggested as a pathogenetic link between periodontal disease and atherosclerosis. The purpose of this study was to examine the systemic effect of a non‐surgical therapy on white blood cell count (WBC count) and differential blood count in smoking and non‐smoking generalized aggressive periodontitis (GAP) patients.Methods: 27 adult periodontitis patients (13 smokers and 14 non‐smokers) with previously untreated GAP were subjected to 3 sessions of oral hygiene procedure. Afterwards, the patients were treated by scaling and root planing under local anaesthesia. Periodontal examinations were performed after supragingival pretreatment and three months after subgingival therapy. Pocket probing depth (PPD) and relative attachment level (RAL) were measured with Florida probe and disc probe. Accompanying clinical evaluation venous blood samples were taken to analyse the WBC counts and differential blood counts. For statistical analysis non‐parametric tests were utilized.Results: No clinical or demographic differences were found between smokers (n=13) and non‐smokers (n=14). PPD, bleeding on probing (BoP) and suppuration improved significantly after therapy both in smokers and non‐smokers. Following periodontal treatment WBC counts, neutrophil and platelet counts decreased significantly in non‐smokers (p≤0.004), while in smokers only platelet counts were significantly reduced (p=0.006). Non‐smokers showed a significantly higher reduction of WBC counts (p=0.005) and neutrophils (p=0.001) compared to smokers.Conclusion: The results indicate that a therapeutical intervention may have a systemic effect on the blood count in GAP patients. This effect seems to differ between smokers and non‐smokers.
Metastatic breast carcinoma mimicking periodontal disease on radiographs
Authors: Ögˇütcen‐Toller M. , Metin M. , Yıldız L. 
Published: March 2003

AbstractBackground: Tumors can metastasize to the jaws, oral soft tissues, or salivary glands. Metastasis from distant organs and tissues to the jaws or oral cavity is considered rare.Results: The mandible is the most common site of metastasis of the oral and maxillofacial region. Radiographically, there is usually an area of radiolucency with hazy outline. Such lesions are manifested as radiopaque or sclerotic areas.Conclusions: Radiographic view of a tumor in the present case mimicked a periodontal lesion with an osteolytic area around the roots.