bluuebunny/crossref_metadata_2025_split huggingface dataset:
Faecalibacterium duncaniae A2-165 regulates the expression of butyrate synthesis, ferrous iron uptake, and stress-response genes based on acetate consumption
Authors: Verstraeten Sophie , Layec Séverine , Auger Sandrine , Juste Catherine , Henry Céline , Charif Sawiya , Jaszczyszyn Yan , Sokol Harry , Beney Laurent , Langella Philippe , Thomas Muriel , Huillet Eugénie 
Published: January 2024

AbstractThe promising next-generation probiotic Faecalibacterium prausnitzii is one of the most abundant acetate-consuming, butyrate-producing bacteria in the healthy human gut. Yet, little is known about how acetate availability affects this bacterium’s gene expression strategies. Here, we investigated the effect of acetate on temporal changes in the transcriptome of F. duncaniae A2-165 cultures using RNA sequencing. We compared gene expression patterns between two growth phases (early stationary vs. late exponential) and two acetate levels (low: 3 mM vs. high: 23 mM). Only in low-acetate conditions, a general stress response was activated. In high-acetate conditions, there was greater expression of genes related to butyrate synthesis and to the importation of B vitamins and iron. Specifically, expression was strongly activated in the case of the feoAABC operon, which encodes a FeoB ferrous iron transporter, but not in the case of the feoAB gene, which encodes a second putative FeoAB transporter. Moreover, excess ferrous iron repressed feoB expression but not feoAB. Lastly, FeoB but not FeoAB peptides from strain A2-165 were found in abundance in a healthy human fecal metaproteome. In conclusion, we characterized two early-stationary transcriptomes based on acetate consumption and this work highlights the regulation of feoB expression in F. duncaniae A2-165.
Targeting the dermis for melasma maintenance treatment
Authors: Han Hee Jeong , Kim Jin Cheol , Park Young Joon , Kang Hee Young 
Published: January 2024

AbstractMelasma relapse is almost common after discontinuation of conventional treatment. Recent studies suggesting that photoaging dermis is the main pathomechanism of melasma, emphasize the dermal targeting therapy. We investigated maintenance effect of microneedling radiofrequency (RF) for melasma treatment. Subjects with melasma were administered oral tranexamic acid and triple combination cream for 2 months and a randomly assigned half face was treated with RF. After discontinuation of conventional therapy, the half face RF continued monthly over 6 months. Modified melasma area severity index (mMASI) score and L* value by a chromameter were collected monthly. Fifteen subjects were enrolled and eleven completed the 8-month study. At 2nd month of conventional therapy, all subjects showed improvement with a 64% reduction in mMASI score. With continuous RF treatment, the improvement was well maintained; whereas in untreated side, the Δ L* gradually decreased, returning to the baseline after the conventional therapy ended. The continuous microneedling RF therapy is beneficial in maintaining the conventional therapy of melasma suggesting the protective effect of dermal targeting therapy in melasma development.(Clinical Trial registration number: NCT05710068, date of first registration: 02/02/2023).
Incidence and prognostic significance of newly-diagnosed atrial fibrillation among older U.S. veterans hospitalized with COVID-19
Authors: Ko Darae , Treu Timothy M. , Tarko Laura , Ho Yuk-Lam , Preis Sarah R. , Trinquart Ludovic , Gagnon David R. , Monahan Kevin M. , Helm Robert H. , Orkaby Ariela R. , Lubitz Steven A. , Bosch Nicholas A. , Walkey Allan J. , Cho Kelly , Wilson Peter W. F. , Benjamin Emelia J. 
Published: January 2024

AbstractMost prior studies on the prognostic significance of newly-diagnosed atrial fibrillation (AF) in COVID-19 did not differentiate newly-diagnosed AF from pre-existing AF. To determine the association between newly-diagnosed AF and in-hospital and 30-day mortality among regular users of Veterans Health Administration using data linked to Medicare. We identified Veterans aged ≥ 65 years who were hospitalized for ≥ 24 h with COVID-19 from 06/01/2020 to 1/31/2022 and had ≥ 2 primary care visits within 24 months prior to the index hospitalization. We performed multivariable logistic regression analyses to estimate adjusted risks, risk differences (RD), and odds ratios (OR) for the association between newly-diagnosed AF and the mortality outcomes adjusting for patient demographics, baseline comorbidities, and presence of acute organ dysfunction on admission. Of 23,299 patients in the study cohort, 5.3% had newly-diagnosed AF, and 29.2% had pre-existing AF. In newly-diagnosed AF adjusted in-hospital and 30-day mortality were 16.5% and 22.7%, respectively. Newly-diagnosed AF was associated with increased mortality compared to pre-existing AF (in-hospital: OR 2.02, 95% confidence interval [CI] 1.72–2.37; RD 7.58%, 95% CI 5.54–9.62) (30-day: OR 1.86; 95% CI 1.60–2.16; RD 9.04%, 95% CI 6.61–11.5) or no AF (in-hospital: OR 2.24, 95% CI 1.93–2.60; RD 8.40%, 95% CI 6.44–10.4) (30-day: 2.07, 95% CI 1.80–2.37; RD 10.2%, 95% CI 7.89–12.6). There was a smaller association between pre-existing AF and the mortality outcomes. Newly-diagnosed AF is an important prognostic marker for patients hospitalized with COVID-19. Whether prevention or treatment of AF improves clinical outcomes in these patients remains unknown.
Percutaneous transaxillary approach through the first segment of the axillary artery for the Impella-supported PCI Versus TAVR
Authors: Sacha Jerzy , Krawczyk Krzysztof , Gwóźdź Witold , Lipski Przemysław , Milejski Wojciech , Feusette Piotr , Cisowski Marek , Gierlotka Marek 
Published: January 2024

AbstractPercutaneous transaxillary approach (PTAX) through the first segment of the axillary artery is not widely recognized as a safe method. Furthermore, PTAX has never been directly compared between Impella-supported percutaneous coronary interventions (Impella-PCI) and transcatheter aortic valve replacement (TAVR). This study evaluated the feasibility and safety of PTAX through the first axillary segment in Impella-PCI versus TAVR. In cases where standard imaging guidance was insufficient, a technique involving puncturing the axillary artery “on-the-balloon” was employed. The endpoints were bleeding and vascular complications, as defined by BARC and VARC-3 criteria. PTAX was successfully performed in all 46 attempted cases: 23 for Impella-PCI and 23 for TAVR. Strict adherence to BARC and VARC-3 criteria led to the frequent identification of major bleeding (57%) and a moderately frequent diagnosis of vascular complications (17%). These incidences were primarily based on post-procedural hemoglobin reduction (> 3 g/dl) but not overt bleeding. The Impella group exhibited a higher rate of BARC 3b bleeding due to a greater hemoglobin decline resulting from the prolonged implant duration and PCI itself. Left axillary access was linked to smaller blood loss. Bleeding and vascular complications, as per BARC and VARC-3 definitions, did not affect short-term prognosis, with only 3 Impella patients succumbing to heart failure unrelated to the procedures during one-month follow-up period.
Added value of positive intraluminal contrast CT over fluoroscopic examination for detecting gastrointestinal leakage after gastrointestinal surgery
Authors: Kim Min Gwan , Kim Se Hyung , Jeon Sun Kyung , Han Seungchul 
Published: January 2024

AbstractWe aimed to evaluate the added value of positive intraluminal contrast computed tomography (CT) over fluoroscopy in detecting anastomotic leakage after gastrointestinal (GI) surgery. A total of 141 GI surgery patients who underwent fluoroscopic examination and CT were included. Two radiologists reviewed the fluoroscopic images with and without CT to determine anastomotic leakage on a 5-point confidence scale and graded the leakage on a 4-point grading system. The hospital stay duration and treatment type were recorded. The radiologists’ diagnostic performance in determining leakage was compared using the receiver operating characteristics analysis, and interobserver agreement was analyzed. Fifty-three patients developed GI leakage. When CT was added to the fluoroscopic images, the area under the curve (AUC) values significantly increased for both reviewers. The interobserver agreement for leakage between the two reviewers was excellent and improved with the addition of CT (weighted kappa value, 0.869 versus 0.805). Postoperative intervention was more frequently performed (P < 0.001), and patients with leakage had a significantly longer mean postoperative hospital stay (45 days vs. 27 days) (P = 0.003). Thus, positive intraluminal contrast CT provides added value over fluoroscopic examination for detecting GI leakage in patients undergoing GI tract surgery, increasing AUC values, and improving interobserver agreement.
Association between body mass index and respiratory symptoms in US adults: a national cross-sectional study
Authors: Sun Yuefeng , Zhang Yueyang , Liu Xiangyang , Liu Yingying , Wu Fan , Liu Xue 
Published: January 2024

AbstractThe correlation between body mass index (BMI) and the development of cough, shortness of breath, and dyspnea is unclear. Therefore, this study aimed to investigate the association between these parameters. Data from individuals who participated in the National Health and Nutrition Examination Survey between 2003 and 2012 were analyzed. Weighted logistic regression analysis and smoothed curve fitting were used to examine the correlation between BMI and respiratory symptoms. In addition, the relationship between BMI, chronic obstructive pulmonary disease (COPD), and bronchial asthma was examined. Stratified analysis was used to discover inflection points and specific groups. Weighted logistic regression and smoothed curve fitting revealed a U-shaped relationship between BMI and respiratory symptoms. The U-shaped relationship in BMI was also observed in patients with bronchial asthma and COPD. Stratified analysis showed that the correlation between BMI and wheezing and dyspnea was influenced by race. In addition, non-Hispanic black individuals had a higher risk of developing cough than individuals of the other three races [OR 1.040 (1.021, 1.060), p < 0.0001], and they also exhibited an inverted U-shaped relationship between BMI and bronchial asthma. However, the association of BMI with cough, wheezing, dyspnea, COPD, and asthma was not affected by sex. High or low BMI was associated with cough, shortness of breath, and dyspnea, and has been linked to bronchial asthma and COPD. These findings provide new insights into the management of respiratory symptoms and respiratory diseases.
Childhood conduct problems and parent–child talk during social and nonsocial play contexts: a naturalistic home-based experiment
Authors: Sun Sydney , Plate Rista C. , Jones Callie , Rodriguez Yuheiry , Katz Chloe , Murin Melissa , Pearson Jules , Parish-Morris Julia , Waller Rebecca 
Published: January 2024

AbstractParent–child interactions are a critical pathway to emotion socialization, with disruption to these processes associated with risk for childhood behavior problems. Using computational linguistics methods, we tested whether (1) play context influenced parent–child socioemotional language, and (2) child conduct problems or callous-unemotional traits were associated with patterns of socioemotional or nonsocial language across contexts. Seventy-nine parent–child dyads (children, 5–6 years old) played a socioemotional skills (“social context”) or math (“nonsocial context”) game at home. We transcribed and analyzed game play, which had been audio recorded by participants. The social context elicited more socioemotional and cognitive words, while the nonsocial context elicited more mathematical words. The use of socioemotional language by parents and children was more strongly correlated in the social context, but context did not moderate the degree of correlation in cognitive or mathematical word use between parents and children. Children with more conduct problems used fewer socioemotional words in the social context, while children with higher callous-unemotional traits used fewer cognitive words in both contexts. We highlight the role of context in supporting socioemotionally rich parent–child language interactions and provide preliminary evidence for the existence of linguistic markers of child behavior problems. Our results also inform naturalistic assessments of parent–child interactions and home-based interventions for parents and children facing socioemotional or behavioral challenges.
Approaching expert-level accuracy for differentiating ACL tear types on MRI with deep learning
Authors: Xue Yang , Yang Shu , Sun Wenjie , Tan Hui , Lin Kaibin , Peng Li , Wang Zheng , Zhang Jianglin 
Published: January 2024

AbstractTreatment for anterior cruciate ligament (ACL) tears depends on the condition of the ligament. We aimed to identify different tear statuses from preoperative MRI using deep learning-based radiomics with sex and age. We reviewed 862 patients with preoperative MRI scans reflecting ACL status from Hunan Provincial People’s Hospital. Based on sagittal proton density-weighted images, a fully automated approach was developed that consisted of a deep learning model for segmenting ACL tissue (ACL-DNet) and a deep learning-based recognizer for ligament status classification (ACL-SNet). The efficacy of the proposed approach was evaluated by using the sensitivity, specificity and area under the receiver operating characteristic curve (AUC) and compared with that of a group of three orthopedists in the holdout test set. The ACL-DNet model yielded a Dice coefficient of 98% ± 6% on the MRI datasets. Our proposed classification model yielded a sensitivity of 97% and a specificity of 97%. In comparison, the sensitivity of alternative models ranged from 84 to 90%, while the specificity was between 86 and 92%. The AUC of the ACL-SNet model was 99%, demonstrating high overall diagnostic accuracy. The diagnostic performance of the clinical experts as reflected in the AUC was 96%, 92% and 88%, respectively. The fully automated model shows potential as a highly reliable and reproducible tool that allows orthopedists to noninvasively identify the ACL status and may aid in optimizing different techniques, such as ACL remnant preservation, for ACL reconstruction.
Functional characterization of the dimeric form of PDGF-derived fusion peptide fabricated based on theoretical arguments
Authors: Sadeghi-Ardebili Maryam , Hasannia Sadegh , Dabirmanesh Bahareh , Khavari-Nejad Ramazan Ali 
Published: January 2024

AbstractA skin wound leads to the loss of skin integrity and the influx of pathogens into the tissue. Platelet-derived growth factors (PDGFs) are cytokines released from alpha granules during wound healing and interact with their cell surface receptors and activate signals involved in chemotaxis, growth, proliferation, and differentiation pathways. Due to the low stability of growth factors (GFs), a new peptide-derived PDGF-BB was designed, expressed in the Shuffle strain of E. coli, and purified by Ni-NTA agarose affinity column chromatography. The effect of fusion peptide was then evaluated on L929 fibroblast cells and animal models with skin lesions. In vitro, studies showed that the peptide led to an increase in the migration of fibroblast cells in the scratch assay. Its positive effect on wound healing was also observed in the skin-injured rats after 3, 7, and 12 days. A significant rise in neutrophils and granular tissue formation, re-epithelialization, angiogenesis, and collagen formation was exhibited on the third day of treatment when compared to the control group. The results showed that, despite reducing PDGF size, the fusion peptide was able to maintain at least some of the known functions attributed to full-length PDGF and showed positive results in wound healing.
Unveiling dysregulated lncRNAs and networks in non-syndromic cleft lip with or without cleft palate pathogenesis
Authors: Wu Caihong , Liu Haojie , Zhan Zhuorong , Zhang Xinyu , Zhang Mengnan , You Jiawen , Ma Junqing 
Published: January 2024

AbstractNon-syndromic cleft lip with or without cleft palate (NSCL/P) is a common congenital facial malformation with a complex, incompletely understood origin. Long noncoding RNAs (lncRNAs) have emerged as pivotal regulators of gene expression, potentially shedding light on NSCL/P's etiology. This study aimed to identify critical lncRNAs and construct regulatory networks to unveil NSCL/P's underlying molecular mechanisms. Integrating gene expression profiles from the Gene Expression Omnibus (GEO) database, we pinpointed 30 dysregulated NSCL/P-associated lncRNAs. Subsequent analyses enabled the creation of competing endogenous RNA (ceRNA) networks, lncRNA-RNA binding protein (RBP) interaction networks, and lncRNA cis and trans regulation networks. RT-qPCR was used to examine the regulatory networks of lncRNA in vivo and in vitro. Furthermore, protein levels of lncRNA target genes were validated in human NSCL/P tissue samples and murine palatal shelves. Consequently, two lncRNAs and three mRNAs: FENDRR (log2FC = − 0.671, P = 0.040), TPT1-AS1 (log2FC = 0.854, P = 0.003), EIF3H (log2FC = − 1.081, P = 0.041), RBBP6 (log2FC = 0.914, P = 0.037), and SRSF1 (log2FC = 0.763, P = 0.026) emerged as potential contributors to NSCL/P pathogenesis. Functional enrichment analyses illuminated the biological functions and pathways associated with these lncRNA-related networks in NSCL/P. In summary, this study comprehensively delineates the dysregulated transcriptional landscape, identifies associated lncRNAs, and reveals pivotal sub-networks relevant to NSCL/P development, aiding our understanding of its molecular progression and setting the stage for further exploration of lncRNA and mRNA regulation in NSCL/P.
Seasonal to decadal variations of precipitation oxygen isotopes in northern China linked to the moisture source
Authors: Lin Fangyuan , Zhang Qiong , Sinha Ashish , Wang Zhenqian , Axelsson Josefine , Chen Lvfan , Wang Tianli , Tan Liangcheng 
Published: January 2024

AbstractA precise characterization of moisture source and transport dynamics over the inland margins of monsoonal China is crucial for understanding the climatic significance of precipitation oxygen isotope (δ18Op) variability preserved in the regional proxy archives. Here, we use a general circulation model with an embedded water-tagging module to quantify the role of moisture dynamics on the seasonal to decadal variations of δ18Opin northern China. Our data indicate that during the non-monsoon season, the δ18Opvariability is dominated by the temperature effect. Conversely, in the summer monsoon season, the moisture contributions from the low-latitude land areas (LLA), the Pacific Ocean (PO), and the North Indian Ocean (NIO) override the temperature effect and influence the summer δ18Op. Intensified upstream convection along the NIO moisture transport pathway results in a more negative summer δ18Opcompared to moisture transported from the PO and LLA regions. Our analysis shows a decadal shift in summer δ18Oparound the mid-1980s, marking changes in the relative contribution of oceanic moisture from PO and NIO in response to changes in the atmospheric circulation patterns influenced by the Pacific Decadal Oscillation. We suggest that such decadal-scale δ18Opvariability can be recorded in the natural archives from the region, which can provide valuable insights into understanding past climate variability.
Squalamine reverses age-associated changes of firing patterns of myenteric sensory neurons and vagal fibres
Authors: McVey Neufeld Karen-Anne , Mao Yu-Kang , West Christine L. , Ahn Matthew , Hameed Hashim , Iwashita Eiko , Stanisz Andrew M. , Forsythe Paul , Barbut Denise , Zasloff Michael , Kunze Wolfgang A. 
Published: January 2024

AbstractVagus nerve signaling is a key component of the gut-brain axis and regulates diverse physiological processes that decline with age. Gut to brain vagus firing patterns are regulated by myenteric intrinsic primary afferent neuron (IPAN) to vagus neurotransmission. It remains unclear how IPANs or the afferent vagus age functionally. Here we identified a distinct ageing code in gut to brain neurotransmission defined by consistent differences in firing rates, burst durations, interburst and intraburst firing intervals of IPANs and the vagus, when comparing young and aged neurons. The aminosterol squalamine changed aged neurons firing patterns to a young phenotype. In contrast to young neurons, sertraline failed to increase firing rates in the aged vagus whereas squalamine was effective. These results may have implications for improved treatments involving pharmacological and electrical stimulation of the vagus for age-related mood and other disorders. For example, oral squalamine might be substituted for or added to sertraline for the aged.
A single-cell atlas of the aging mouse ovary
Authors: Isola José V. V. , Ocañas Sarah R. , Hubbart Chase R. , Ko Sunghwan , Mondal Samim Ali , Hense Jessica D. , Carter Hannah N. C. , Schneider Augusto , Kovats Susan , Alberola-Ila José , Freeman Willard M. , Stout Michael B. 
Published: January 2024

AbstractOvarian aging leads to diminished fertility, dysregulated endocrine signaling and increased chronic disease burden. These effects begin to emerge long before follicular exhaustion. Female humans experience a sharp decline in fertility around 35 years of age, which corresponds to declines in oocyte quality. Despite a growing body of work, the field lacks a comprehensive cellular map of the transcriptomic changes in the aging mouse ovary to identify early drivers of ovarian decline. To fill this gap we performed single-cell RNA sequencing on ovarian tissue from young (3-month-old) and reproductively aged (9-month-old) mice. Our analysis revealed a doubling of immune cells in the aged ovary, with lymphocyte proportions increasing the most, which was confirmed by flow cytometry. We also found an age-related downregulation of collagenase pathways in stromal fibroblasts, which corresponds to rises in ovarian fibrosis. Follicular cells displayed stress-response, immunogenic and fibrotic signaling pathway inductions with aging. This report provides critical insights into mechanisms responsible for ovarian aging phenotypes. The data can be explored interactively via a Shiny-based web application.
Polarisation of Climate and Environmental Attitudes in the United States, 1973-2022
Authors: Smith E. Keith , Bognar M. Julia , Mayer Adam P. 
Published: January 2024

AbstractSince the early 1990s, increasing political polarisation is among the greatest determinants of individual-level environmental and climate change attitudes in the United States. But several patterns remain unclear: are historical patterns of polarisation largely symmetrical (equal) or is rather asymmetrical (where one set of partisans shifts more than others)? How have polarisation patterns have changed over time? How generalizable are polarization patterns across different environmental and climate change attitudes? We harmonised four unique sets of historical, pooled cross-sectional survey data from the past 50 years to investigate shifts across seven distinct measures of citizen environmental and climate change attitudes. We find that contemporary attitudes are polarised symmetrically, with Democrats (higher) and Republicans (lower) attitudes are equidistant from the median. But the historical trends in polarisation differ by attitudes and beliefs. In particular, we find evidence of two distinct historical patterns of asymmetric polarisation within environmental and climate change attitudes: first, with Republicans becoming less pro-environmental, beginning in the early 1990s, and second, a more recent greening of Democratic environmental attitudes since the mid-2010s. Notably, recent increases in pro-environmental attitudes within Democrats is a potentially optimistic finding, providing opportunities towards overcoming decades-long inertia in climate action. These findings provide a foundation for further research avenues into the factors shaping increased pro-environmental attitudes within Democrats.