bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of hTREK-1 by polyunsaturated fatty acids does not only involve membrane tension
Authors: BECHARD Emilie , AREL Elodie , BRIDE Jamie , LOURADOUR Julien , BUSSY Xavier , ELLOUMI Anis , VIGOR Claire , SOULE Pierre , OGER Camille , GALANO Jean-Marie , DURAND Thierry , GUENNEC Jean-Yves LE , MOHA-OU-MAATI Hamid , DEMION Marie 
Published: January 2024

Abstract
TREK-1 is a mechanosensitive channel activated by polyunsaturated fatty acids (PUFAs). Its activation is supposed to be linked to changes in membrane tension following PUFAs insertion. Here, we compared the effect of numerous fatty acids and ML402 on TREK-1 channel activation using the whole cell and the inside-out configurations of the patch-clamp technique. Firstly, TREK-1 activation by PUFAs is variable and related to the variable constitutive activity of TREK-1. We observed no correlation between TREK-1 activation and acyl chain length or number of double bonds suggesting that the bilayer-couple hypothesis cannot explain by itself the activation of TREK-1 by PUFAs. The membrane fluidity measurement is not modified by PUFAs at 10 µM. The spectral shift analysis in TREK-1-enriched microsomes indicates a KD,TREK1 at 44 µM of C22:6 n-3. PUFAs display the same activation and reversible kinetics than the direct activator ML402 and activate TREK-1 in both whole-cell and inside-out configurations of patch-clamp suggesting that the binding site of PUFAs is accessible from both sides of the membrane, as for ML402. Finally, we proposed a two steps mechanism: first, insertion into the membrane, with no fluidity or curvature modifications at 10 µM, and then interaction with TREK-1 channel to open it.
Internet, entrepreneurship and high-quality economic development
Authors: Cao Jingbo , Kang Chenyu 
Published: January 2024

Abstract

The Internet, as an important infrastructure for the development of digital economy, is crucial in the process of economic high-quality development. The annual panel data of 284 cities above prefecture level in China from 2009 to 2020 are selected as the research object, and the principal component analysis method is used to measure the indicators of high-quality development of the economy; at the same time, fixed effect, mediation effect, threshold effect and spatial Durbin model are established, and entrepreneurship is included in the research system to explore the Internet, entrepreneurship and high-quality development of the economy The relationship between the Internet, entrepreneurship and economic high-quality development. The results of the study show that: (1) overall, the Internet promotes high-quality economic development; (2) the Internet will promote high-quality economic development by inspiring entrepreneurship, and when entrepreneurship is active to a certain extent, the positive impact of the Internet on high-quality economic development will be more obvious; (3) the level of high-quality development of neighboring economies promotes each other, and the Internet of the surrounding cities has a favorable impact on the high-quality development of the city's economy. favorable impact, this city Internet will, also promote the surrounding economic high-quality development. Therefore, high-quality economic development can be promoted by accelerating the construction of new digital infrastructure, coordinating the layout of Internet infrastructure and improving the incentive mechanism for entrepreneurs.
A study of the effect of quartz-to-cristobalite transformation on SiC generation in metallurgical-grade silicon production
Authors: 田 嘉禾 , Wei Kuixian , Deng Xiaocong , Ma Wenhui 
Published: January 2024

Abstract
Silicon carbide (SiC) is an essential intermediate product formed during the smelting process of metallurgical-grade silicon (MG-Si), and its production efficiency is a key factor in determining the overall efficiency of MG-Si production. In this study, we investigated the effect of quartz-to-cristobalite transformation on SiC generation in industrial silicon production and elucidated the differences in the reaction characteristics of quartz and cristobalite when they interacted with carbonaceous reductants. The experimental results indicated that the rate of direct carbothermal reduction of cristobalite was 1.45 times that of quartz. Moreover, the indirectly formed SiC layer in the cristobalite/C diffusion couple exhibited a thickness of 920.87 µm, which was 1.55 times that in the quartz/C diffusion couple. Both the reaction thermodynamic calculations and crystal transformation theory analysis revealed that the changes in the chemical energy and crystal structure of SiO2 during the phase transformation process reduced the stability of cristobalite compared with quartz at high temperatures. Consequently, cristobalite reacted more easily with C at high temperatures to form SiC and SiO. The results of the study are highly significant for improving the reaction mechanism in the smelting process of MG-Si and enhancing the production efficiency of MG-Si.
Determination and Health Risk Assessment of Carbamate Pesticide Residues in Date Palm fruits (Phoenix dactylifera) Using QuEChERS Method and UHPLC-MS/MS
Authors: Morsi Rana , Ghoudi Kilani , Meetani Mohammed A. 
Published: January 2024

Abstract
This study aimed to investigate carbamate pesticide residues in different varieties of date palm fruits in the UAE, utilizing UHPLC-MS/MS. For sample preparation and clean-up, the efficiency and performance of different QuEChERS dispersive solid-phase extraction kits were compared. Precision and recovery were assessed at 10 𝜇g kg-1 for the three kits, revealing that Kit 2 demonstrated the best performance. The selected QuEChERS method was validated to detect 14 carbamate residues in 55 date samples. The method exhibited strong linearity with R2>0.999 and low LOD (0.01- 0.005 𝜇g kg-1) and LOQ (0.003- 0.04 𝜇g kg-1). Excellent accuracy (recovery: 88-106%) and precision (RSD: 1-11%) were observed, with negligible matrix effect (-4.98-13.26%). All samples contained at least one carbamate residue. While the majority of detected residues were below their MRLs, carbosulfan was found in 21 samples, propoxur in 2 samples, and carbofuran in 1 sample above their MRLs. To assess potential health risks for date consumers, the hazard index (HI) was calculated for carbosulfan, phenmedipham, carbaryl, propoxur, carbofuran, and methomyl. All HI values were below the safety limit of 1.0, indicating that the consumption of dates does not pose a non-carcinogenic health risk for adults and children.
Controlling edwardsiellosis caused by Edwardsiella piscicida and mitigating drug-resistant gene dissemination: Bacteriophage EPP-1, a promising antibiotic alternative
Authors: Han Ganghua , Huang Ting , Liu Xinchun , Liu Ruyin 
Published: January 2024

Abstract
Edwardsiella piscicida causes significant economic losses to the aquaculture industry worldwide. Phage-based biocontrol methods are experiencing a renaissance because of the spread of drug-resistant genes and bacteria resulting from the heavy use of antibiotics. Here, we showed that the novel Edwardsiella phage EPP-1 could achieve comparable efficacy to florfenicol using a zebrafish model of Edwardsiella piscicida infection and could reduce the content of the floR resistance gene in zebrafish excreta. Specifically, phage EPP-1 inhibited bacterial growth in vitro and significantly improved the zebrafish survival rate in vivo (P = 0.0035), achieving an efficacy comparable to that of florfenicol (P = 0.2304). Notably, integrating the results of 16S rRNA sequencing, metagenomic sequencing, and qPCR, although the effects of phage EPP-1 converged with those of florfenicol in terms of the community composition and potential function of the zebrafish gut microbiota, it reduced the floR gene content in zebrafish excreta and aquaculture water. Overall, our study highlights the feasibility and safety of phage therapy for edwardsiellosis control, which has profound implications for the development of antibiotic alternatives to address the antibiotic crisis.
Evaluation of antibacterial and antifungal properties of copper oxide nanoparticles after combination with tissue conditioner used in complete prosthesis
Authors: Nikanjam Saeed , Yeganegi Aria , Alikhani Mohammad-Yousef , Farmany Abbas , Ghiasian Seyed Amir , Hasanzade Roghayeh 
Published: January 2024

Abstract
Background
Tissue conditioners are used for treating and improving the tissues supporting complete dentures. On the other hand, recent advances in nanotechnology have revolutionized various fields of science, including dentistry. Moreover, some nanoparticles exert antimicrobial properties.The present study aimed to investigate the antimicrobial properties of copper oxide nanoparticle-based tissue conditioner used in complete prostheses.
Methods
The present experimental study included 126 tissue conditioner samples with different concentrations of copper oxide nanoparticles (0%, 0.625%, 1.25%, 2.5%, 5%, 10%, 20% w/w). The samples were incubated with Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans in 24-well plates for 24 hours. Then, samples from the wells were re-incubated for 24 hours, and the microorganisms were counted.
Results
The culture media containing E. faecalis and P. aeruginosa showed significantly different growth between different nanoparticle concentrations following 24 hours (P < 0.001), showing a reduction in bacterial growth with increased nanoparticle concentration. Both bacteria did not show any growth at the 20% concentration. However, C. albicans showed significant differences in growth between different nanoparticle concentrations following 48 hours (P < 0.001), showing a reduction in growth with increased nanoparticle concentration. Also, the least growth was observed at the 20% concentration.
Conclusions
In the present study, the CuO nanoparticles were made properly in the suitable sizes. Moreover, the tissue conditioners containing copper oxide nanoparticles showed acceptable antimicrobial properties against E. faecalis, P. aeruginosa, and C. albicans.
MRI-based vertebral bone quality score as a novel bone status marker of patients with adolescent idiopathic scoliosis
Authors: Yang Dan-dan , Li Yi , Tian Jiang-yu , Li Ya , Liu Jian , Liu Yun-song , Cao Xin-wen , Liu Chuan 
Published: January 2024

Abstract
Purpose: To evaluate the application of MRI-based L1-L4 vertebral bone quality (VBQ) score in assessing bone mineral density (BMD) for patients with adolescent idiopathic scoliosis (AIS).
Methods: We reviewed the data of AIS patients between January 2021 and October 2023 with MRI, whole-spine plain radiographs, quantitative computed tomography (QCT) and general information. VBQ L1-L4 score was calculated using T1-weighted MRI. Univariate analysis was applied to present the differences between variables of patients with normal group (Z-score>-2.0) and low-BMD group (Z-score≤-2.0). The correlation between the VBQ score and QCT Z-score was analyzed with Pearson correlation test. A multivariate logistic regression model was used to determine the independent factors related to low BMD. Receiver operating characteristic curve (ROC) was drawn to analyze the diagnostic performance of VBQ L1-L4 score in distinguishing low BMD.
Results: A total of 136 AIS patients (mean age was 14.84±2.10 years) were included, of which 41 had low BMD. The low-BMD group had a significantly higher VBQL1-L4 score than that in normal group (3.48±0.85 vs 2.62±0.62, P< 0.001). The VBQL1-L4 score was significantly negative correlated with QCT Z score (r = − 0.454, P< 0.001). On multivariate analysis, VBQL1-L4 score was independently associated with low BMD (OR: 4.134, 95% CI: 2.136–8.000, P<0.001). The area under the ROC curve indicated that the diagnostic accuracy of the VBQL1-L4 score for predicting low BMD was 81%. A sensitivity of 65.9% with a specificity of 88.4% could be achieved for distinguishing low BMD by setting the VBQL1-L4 score cutoff as 3.18.
Conclusions: The novel VBQL1-L4 score was a promising tool in distinguishing low BMD in patients with AIS and could be useful as opportunistic assessment for screening and complementary evaluation to QCT before surgery.
The Pretreatment Neutrophil-to-Lymphocyte Ratio (NLR) as a Near-term Prognostic Indicator in Patients with Locally Advanced Hepatocellular Carcinoma Treated with Hepatic Artery Infusion Chemotherapy: A Propensity Score Matching Cohort Study
Authors: Liu Weifu , Zhang Kongzhi , Chen Shiguang , Wang Xiaolong , Yu Wenchang 
Published: January 2024

Abstract

Objectives To investigate the value of the pretreatment neutrophil-to-lymphocyte ratio (NLR) for predicting the near-term efficacy of hepatic arterial infusion chemotherapy (HAIC) in patients with locally advanced hepatocellular carcinoma (HCC).
Methods In this retrospective study, data were collected from locally advanced HCC patients treated with HAIC between January 2018 and June 2022. Patients were categorized based on their pretreatment NLRs and analyzed via propensity score matching (PSM). The primary study endpoints were the objective response rate (ORR), progression-free survival (PFS), and safety.
Results This study included 104 patients. Using X-tile software, the optimal pretreatment NLR cutoff was 2.90, and patients were divided into a high NLR subgroup (>2.9) (n=44 patients) and a low NLR subgroup (≤2.9) (n=60 patients). Following PSM, 43 matched pairs were analyzed. Patients with a low pretreatment NLR exhibited a significantly greater ORR (69.8% vs 37.2%, P=0.002) and improved PFS (6.7 months vs 3.8 months, P=0.007) than did those with a high NLR. Both univariate and multivariate regression analyses demonstrated that a high pretreatment NLR is an independent negative prognostic factor for ORR ([HR], 3.464; 95% CI, 1.383-8.678; P=0.008) and PFS ([HR], 1.634; 95% CI, 1.026-2.600; P=0.038). No significant differences in the incidence of adverse events (AEs) were observed between the groups.
Conclusion The pretreatment NLR is a readily obtainable and effective biomarker for predicting the near-term efficacy of HAIC in patients with locally advanced HCC.
Estimation Ion Transport Parameters in PVFM Based Solid Polymer Electrolyte using Trukhan Model
Authors: Pandey Kamlesh , Dwivedi Mrigank Mauli , Sanjay Sharda S. , Asthana Nidhi 
Published: January 2024

Abstract

Biosynthetic route for the synthesis of nanoparticles involving fossil extract is a modest eco-friendly process, the study focused on a relatively new class of functional materials known as polymerized ionic liquids The synthesized nanoparticles were then characterized through UV-Visible, FTIR and X-ray diffractionspectroscopy.Nanoparticles incorporated in polymers providing an exciting base for newer technology for the development of cost effective electrochemical and devices. Poly (vinyl alcohol) (PVAM) is one of the eco-friendly semi-crystalline polymers which has inter- and intra-molecular hydrogen bonds, whose degree is greatly dependent on the texture of the polymer. We have fabricated a naturally plasticized nanopolymer electrolyte membrane with high ionic conductivity using nano MMT incorporated in PVAM. Randomly interwoven nano-fibrous structures are generally preferred for electrolyte systems due to their continuous structure. The use of nano-composites provided a better polymer interface as well as the high free volume size, through which molecules transport occurs in nano-composite membrane matrix. The advantage of a surface comprised of ultra-fine, continuous nano fibres naturally becomes highly porous. Nano composite membrane due to its high porosity becomes a major responsive factor to enhance the ionic conductivity. We have considered a comparative study of these fabricated nano-composites. The structural behaviour of the polymer nano-composite membrane was investigated by XRD, FTIR, optical microscopy and scanning electron microscopic technique. XRD curve revealed the amorphous nature of membrane, which is favourable for the better conduction and sensing behaviour, as the conductivity-frequency plots showed the Universal power law. The asymmetric shape of the Dielectric relaxation plots given a strong evidence of low frequency dispersion and deviation from the pure Debye behaviour.
Shared regulatory function of non-genomic thyroid hormone signaling in echinoderm skeletogenesis
Authors: Taylor Elias , Heyland Andreas 
Published: January 2024

Abstract

Thyroid hormones are crucial regulators of metamorphosis and development in bilaterians, particularly in chordate deuterostomes. Recent evidence suggests a role for thyroid hormone signaling, principally via 3,5,3',5'-Tetraiodo-L-thyronine (T4), in the regulation of metamorphosis, programmed cell death and skeletogenesis in echinoids (sea urchins and sand dollars) and sea stars. Here we test whether TH signaling in skeletogenesis is a shared trait of Echinozoa (Echinoida and Holothouroida) and Asterozoa (Ophiourida and Asteroida). We demonstrate dramatic acceleration of skeletogenesis in three classes of echinoderms: sea urchins, sea stars, and brittle stars (echinoids, asteroids, and ophiuroids). Fluorescently labeled thyroid hormone analogues reveal thyroid hormone binding to cells proximal to regions of skeletogenesis in the gut and juvenile rudiment. Immunohistochemistry of phosphorylated MAPK in the presence and absence of TH binding inhibitors suggests that THs may act via phosphorylation of MAPK (ERK1/2) to accelerate skeletogenesis in the three echinoderm groups. Additionally, we detect thyroid hormone binding to the cell membrane and nucleus during metamorphic development in echinoderms. Together, these results indicate that TH regulation of mesenchyme cell activity via integrin-mediated MAPK signaling may be a conserved mechanism for the regulation of skeletogenesis in echinoderm development. Additionally, TH action via a nuclear thyroid hormone receptor may regulate metamorphic development. Our findings shed light on potentially ancient pathways of thyroid hormone activity in echinoids, ophiuroids, and asteroids, or on a signaling system that has been repeatedly co-opted to coordinate metamorphic development in bilaterians.
Exploring Core Themes in Nuclear Pollution Research: Insights from Ibn Khaldun’s Ecological and Economic Perspectives
Authors: Ren Huichan , Abdullah Suhaila 
Published: January 2024

Abstract

This study focuses on a comprehensive examination of the core themes in nuclear pollution research, utilizing Ibn Khaldun’s ecological and economic perspectives. The primary content of this study is to examine Ibn Khaldun’s ecological perspective and explore the probable consequences of nuclear pollution on ecosystems, specifically emphasizing its influence on the sustainable development of human society. Concurrently, the authors utilize Ibn Khaldun’s economic framework to examine the effects of nuclear pollution on the economic equilibrium. Subsequently, the authors extend these effects to assess their impact on the prosperity of metropolitan areas and the sustainability of civilization. Although there has been extensive research on nuclear pollution, approaching the issue from the perspective of Ibn Khaldun’s writing is indeed a novel and groundbreaking endeavor. Due to the interdisciplinary character of this study, the authors employ a bibliometric analysis method, a literature review, and a logical analysis method. The primary objective of this study is to improve understanding of the challenges posed by nuclear contamination and to promote cooperative strategies for achieving environmentally and socio-economically sustainable development.
Correlation between sentinel lymph node biopsy and non-sentinel lymph node metastasis in patients with cN0 breast carcinoma: comparison of invasive ductal carcinoma and invasive lobular carcinoma
Authors: Cipolla Calogero , Lupo Simona , Grassi Nello , Tutino Giuseppe , Greco Martina , Eleonora D’Agati , Gebbia Vittorio , Valerio Maria Rosaria 
Published: January 2024

Abstract
Background: Recent studies have suggested that axillary lymph node dissection (ALND) can be avoided in women with cN0 breast cancer with 1-2 positive sentinel nodes (SLNs). However, these studies included only a few patients with invasive lobular carcinoma (ILC), so the validity of omitting ALDN in these patients remains controversial. This study compared the frequency of non-sentinel lymph nodes (non-SLNs) metastases in ILC and invasive ductal carcinoma (IDC).
Materials Methods: Data relating to a total of 2583 patients with infiltrating breast carcinoma operated at our institution between 2012 and 2023 were retrospectively analyzed: 2242 (86.8%) with IDC and 341 (13.2%) with ILC. We compared the incidence of metastasis to SLNs and non-SLNs between the ILC and IDC cohorts and examined factors that influenced non-SLNs metastasis.
Results: SLN biopsies were performed in 315 patients with ILC and 2018 patients with IDC. Metastases to the SLNs were found in 78/315 (24.8%) patients with ILC and in 460 (22.8%) patients with IDC (p= 0.31). The incidence of metastases to non-SLNs was significantly higher (p = 0.02) in ILC (52/78 - 66.7%) compared to IDC (207/460 - 45%). Multivariate analysis showed that ILC was the most influential predictive factor in predicting the presence of metastasis to non-SLNs.
Conclusions: ILC cases have more non-SLNs metastases than IDC cases in SLN-positive patients. The ILC is essential for predicting non-SLN positivity in macro-metastases in the SLN. The option of omitting ALND in patients with ILC with 1-2 positive SLNs still requires further investigation.
Impact of the serum creatinine and cystatin C ratio for prediction of sarcopenia and prognosis in biliary tract cancer
Authors: Fukushima Ryosuke , Harimoto Norifumi , Okuyama Takayuki , Seki Takaomi , Hoshino Kouki , Hagiwara Kei , Kawai Shunsuke , Ishii Norihiro , Tsukagoshi Mariko , Igarashi Takamichi , Araki Kenichiro , Shirabe Ken 
Published: January 2024

Abstract

Background
Sarcopenia is a poor prognostic factor in cancer patients. In recent years, there have been reports that serum creatinine and cystatin C (Cr/CysC) ratio is associated with sarcopenia. However, the prognostic value of the Cr/CysC ratio in biliary tract cancer is unclear. We evaluated the impact of the Cr/CysC ratio on sarcopenia and prognosis in biliary tract cancer.
Methods
We retrospectively reviewed the records of 190 patients with biliary tract cancer who had undergone surgical resection from January 2017 to March 2023. Frozen serum samples collected at the time of surgery were used to measure CysC. We calculated the Cr/CysC ratio and investigated the relationship with sarcopenia and the prognostic significance.
Results
Serum Cr/CysC ratio was statistically related to both skeletal muscle index and handgrip strength, both of which are diagnostic criteria for sarcopenia (skeletal muscle index: R2 = 0.096, p < 0.001, handgrip strength: R2 = 0.20, p < 0.001). The optimal cutoff value of the Cr/CysC ratio for sarcopenia was 0.848. The low Cr/CysC ratio group was significantly associated with higher preoperative CRP and lower albumin, lower skeletal muscle index, lower handgrip strength, and higher intramuscular adipose tissue content. In multivariate analysis, patients with a low Cr/CysC ratio showed poorer overall survival (hazard ratio 2.60, 95% confidence interval 1.07–6.29, p = 0.033), which was significantly worse than in those with a high Cr/CysC ratio.
Conclusions
The Cr/CysC ratio could be used as a marker to evaluate sarcopenia in patients with biliary tract cancer. Moreover, a low Cr/CysC ratio predicts poor prognosis in biliary tract cancer patients.