bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cholinergic synaptic input to different parts of spiny striatonigral neurons in the rat
Authors: Izzo P. N. , Bolam J. P. 
Published: January 2005

AbstractThe postsynaptic targets of cholinergic boutons in the rat neostriatum were assessed by examination in the electron microscope of boutons that were immunoreactive for choline acetyltransferase, the synthetic enzyme for acetylcholine. These boutons formed symmetrical synaptic specializations with neostriatal neurons. Of 209 immunoreactive synaptic boutons observed in random searches of the neostriatum, 45% made contact with dendritic shafts, 34% with dendritic spines, and 20% with neuronal perikarya. Many of the postsynaptic structures had ultrastructural characteristics of the most common type of striatal neuron, the medium‐size densely spiny neuron. This was confirmed by the examination in the electron microscope of Golgi‐impregnated medium‐size spiny neurons from sections that had also been immunostained for choline acetyltransferase. Immunoreactive boutons formed symmetrical synaptic specializations with all parts of the neurons examined, i.e., perikarya, proximal and distal dendritic shafts, and dendritic spines. Two of the Golgi‐impregnated medium‐size spiny neurons that received input from the cholinergic boutons were also retrogradely labelled with horseradish peroxidase that had been injected into the substantia nigra, they were thus further characterized as striatonigral neurons. Similarly, seven retrogradely labelled perikarya of striatonigral neurons were found to receive input from the cholinergic boutons.It is concluded that cholinergic boutons in the neostriatum form synaptic specializations and that one of their major targets is the medium‐size densely spiny neuron that projects to the substantia nigra. The topography of the cholinergic afferents of these cells is distinctly different from that of other boutons derived from local neurons and from boutons that form asymmetrical synaptic specializations, but it is similar to that of the dopaminergic boutons originating from neurons in the substantia nigra.
Connections of the primary auditory cortex in the common marmoset, Callithrix jacchus jacchus
Authors: Aitkin Lindsay M. , Kudo Motoi , Irvine Dexter R. F. 
Published: January 2005

AbstractThe afferent and efferent connections of the primary auditory cortex (AI) of common marmosets were traced following small injections of wheat germ agglutinin conjugated with horseradish peroxidase (WGA‐HRP) made at best frequency (BF)‐defined sites in the AI. After the injections the animals remained anesthetized for 15–23 hours; they were then perfused transcardially with fixative and the brains were processed for WGA‐HRP reaction product.Examination of the disposition of labelled material revealed the following results. First, patches of terminal labelling, and to a lesser extent retrograde labelling, were found outside the injection site in the ipsilateral cortex rostral and caudal to the AI. Second, the region of the contralateral cortex corresponding to the injection site contained labelled terminals throughout the depth of the cortex; labelled neurons were found in the middle layers. Third, in each experiment a discrete region of the medial geniculate body (MG) contained retrogradely labelled neurons interspersed with anterogradely labelled terminals. These regions had a banded appearance, were found in the dorsal and rostral half of the MG, and shifted in location progressively dorsal and as the injection site BF increased.The presence of projection zones rostral and caudal to the AI of marmosets, and the disposition of the MG sources of projection in relation to BF, are similar to observations made on other New World monkeys. The ipsilateral corticocortical projections confirm electrophysiological evidence suggesting the existence of auditory fields rostral and caudal to the AI. The thalamocortical auditory system of the marmoset appears relatively simple, with a comparatively undifferentiated MG projecting to a cortical auditory system dominated by a large AI.
Localization and characterization of angiotensin II receptor binding and angiotensin converting enzyme in the human medulla oblongata
Authors: Allen Andrew M. , Chai Siew Yeen , Clevers Jenni , McKinley Michael J. , Paxinos George , Mendelsohn Frederick A. O. 
Published: January 2005

AbstractAngiotensin II receptor and angiotensin converting enzyme distributions in the human medulla oblongata were localised by quantitative in vitro autoradiography. Angiotensin II receptors were labelled with the antagonist analogue 125I‐[Sar1, Ile8] All while angiotensin converting enzyme was labelled with 125I‐351A, a derivative of the specific converting enzyme inhibitor, lisinopril.Angiotensin II receptor binding and angiotensin converting enzyme are present in high concentrations in the nucleus of the solitary tract, the dorsal motor nucleus of vagus, the rostral and caudal ventrolateral reticular nucleus, and in a band connecting the dorsal and ventral regions.In the rostral and caudal ventrolateral reticular nucleus, angiotensin II receptors are distributed in a punctate pattern that registers with neuronal cell bodies. The distribution and density of these cell bodies closely resemble those of catecholamine‐containing neurones mapped by others. In view of the known interactions of angiotensin II with both central and peripheral catecholamine‐containing neurons of laboratory animals, the current anatomical findings suggest similar interactions between these neuroactive compounds in the human central nervous system.The presence of angiotensin II receptors and angiotensin converting enzyme in the nucleus of the solitary tract, dorsal motor nucleus of vagus, and rostral and caudal ventrolateral reticular nucleus demonstrates sites for central angiotensin II to exert its known actions on vasopressin release and autonomic functions including blood pressure control. These data also suggest a possible interaction between angiotensin II and central catechol‐eminergic systems.
Morphology of neurons in area 4γ of the cat's cortex studied with intracellular injection of HRP
Authors: Ghosh S. , Fyffe R. E. W. , Porter R. 
Published: January 2005

AbstractNeurons in laminae II, III, V, and VI of area 4γ of the cat motor cortex were studied following intracellular penetration with an HRP‐filled microelectrode. Antidromic and synaptic responses produced by stimulation of the cerebral peduncles and/or of the ventrolateral nucleus of the thalamus were investigated. Horseradish peroxidase was then iontophoresed into the same neurons to allow examination of their detailed morphology.The morphology of pyramidal neurons whose somata were located in a particular lamina was similar but differed from that of pyramidal neurons in other laminae. The modified pyramidal neurons of lamina II had a truncated apical dendrite or did not possess an obvious apical dendrite, even though the ascending dendritic branches were longer and more extensive than the “basal” branches. As was the case for the pyramidal cells in other laminae, the axons of these lamina II modified pyramidal cells descended toward the white matter; their somata were generally pyramidal in shape; and their dendrites were spiny.All pyramidal neurons except some of lamina VI had ascending dendrites which terminated in a tuft in lamina I, subpially.No intracortical collaterals were seen originating from the axons of lamina II or of lamina VI pyramidal neurons.Lamina III pyramidal neurons had extensive short and long axon collaterals which contributed synaptic boutons to all laminae of the cortex. Pyramidal neurons of lamina V had fewer axon collaterals whose synaptic boutons were restricted to laminae V and VI.All somata of pyramidal tract neurons (PTNs), identified by antidromic responses from peduncular stimulation, were located in lamina V, except for one which was located in lamina VI.Recurrent collaterals of pyramidal neurons were activated by peduncular stimulation. Recurrent excitatory postsynaptic potentials (epsps) could be evoked in fast PTNs, slow PTNs, other pyramidal neurons of lamina V, and pyramidal neurons of lamina VI at latencies between 1.3 and 6.25 msec. In some slow PTNs, a recurrent inhibitory postsynaptic potential of long duration was the predominant response. Stimulation of the ventrolateral nucleus of the thalamus resulted in epsps in pyramidal neurons of lamina III, V, and VI at latencies between 1.0 and 5.0 msec.
Ultrastructure of mouse foliate taste buds: Synaptic and nonsynaptic interactions between taste cells and nerve fibers
Authors: Royer Suzanne M. , Kinnamon John C. 
Published: January 2005

AbstractHigh voltage electron microscopy and conventional transmission electron microscopy were used to examine the ultrastructure of foliate taste buds of mice. Computer‐assisted, three‐dimensional reconstructions from serial sections were used to visualize regions of interaction between taste cells and nerve fibers.Based on criteria previously established for murine vallate taste buds (Kinnamon et al., '85), foliate taste cells were classified as dark, light, or intermediate depending on their cytoplasmic content and the characteristics of their nuclei. Cells of foliate taste buds display a continuous range of morphologies, from “typical” dark cells to “typical” light cells. Cells of dark, intermediate, and light morphologies all make afferent synapses onto nerve processes, suggesting that cells of all 3 types are sensory in function. Synapses between taste cells and nerve processes may be either macular or fingerlike in shape. No efferent synapses were found.In addition to conventional synapses, taste cells exhibit 2 other types of specializations at sites of apposition with nerve fibers: subsurface cisternae and atypical mitochondria. Subsurface cisternae are narrow sacs of endoplasmic reticulum that are closely apposed to the inner leaflet of the taste cell membrane. Possible functions of subsurface cisternae include synthesis of synaptic membrane components, modification of the electrical or adhesive properties of the taste cell membrane, and exchange of trophic factors with nerve processes. Atypical mitochondria are usually much larger than typical taste cell mitochondria, and their cristae often display a swollen, twisted configuration. These mitochondria are closely apposed to the inside of the taste cell membrane adjacent to nerve fibers. Atypical mitochondria may be providing unusual amounts of energy for metabolic reactions in their vicinities or participating in calcium buffering in the taste cell.Within taste cells, presynaptic specializations, subsurface cisternae, and mitochondria are often clustered together to form “synaptic ensembles.” We hypothesize that the functions served by the subsurface cisternae and mitochondria, as well as synaptic transmission, may be important in interactions between taste cells and nerve fibers.
Afferent innervation of outer hair cells in adult cats: I. Light microscopic analysis of fibers labeled with horseradish peroxidase
Authors: Simmons D. D. , Liberman M. C. 
Published: January 2005

AbstractOuter spiral fibers (OSFs), the afferent innervation of the outer hair cells (OHCs), were retrogradely labeled following horseradish peroxidase injections into the cat's auditory nerve. The peripheral branching patterns of 85 OSFs from adult cochleas were reconstructed. Fibers contacted OHCs via terminal or en passant swellings; however, the latter were seen exclusively in the apical half of the cochlea. Many OSFs also gave off branches ending on structures other than OHCs. Fibers in the cochlear apex were much more highly branched than in the base. Most fibers contacted only one row of OHCs, and more fibers contacted row 1 than row 2 or row 3 OHCs. Third‐row fibers were the most highly branched in all cochlear regions. These results are consistent with a growing body of morphological evidence that suggests that the peripheral branching patterns of OSFs may be fundamentally similar in all mammalian ears.
Ultrastructural study of large efferent neurons in the superior colliculus of the cat after retrograde labeling with horseradish peroxidase
Authors: Behan M. , Appell P. P. , Graper M. J. 
Published: January 2005

AbstractThe ultrastructure of large neurons in the stratum griseum intermedium of the cat superior colliculus was examined following injections of horseradish peroxidase (HRP) into the dorsal tegmental decussation. Four HRP‐labeled cells were selected, and the synaptology of their cell bodies and selected regions of proximal and distal dendrites was examined. The four neurons represent four morphologically distinct cell types: multipolar radiating, tufted, large vertical, and medium‐sized trapezoid radiating. These four neurons correspond with cell types X1, X2, X3, and T1 respectively, according to the recent classification of neurons in the superior colliculus of the cat by Moschovakis and Karabelas (J. Comp Neurol. 239:276–308, '85).The three X type neurons are similar in having 83% of their somata and over 74% of their proximal dendrites contacted by synaptic profiles. Distal dendrites of the X type neurons, however, receive fewer synaptic contacts. In contrast, in the T1 cell, only 69% of the soma membrane is contacted by synaptic profiles, and the synaptic coverage on proximal and distal dendrites does not vary much from this. Of the eight types of synaptic terminals described in the stratum griseum intermedium of the cat superior colliculus by Norita (J. Comp. Neurol. 190:29–48, '80), only five are found in contact with the X and T type efferent neurons described here. There are some regional differences in terminal distribution, although each terminal is represented on each cell. Type III terminals (small, contain mostly pleomorphic vesicles, and make symmetrical contacts) are the most abundant on cell bodies and dendrites of all four cell types. Terminal types II (medium‐sized, containing round and flattened vesicles, and making asymmetrical contacts), and IV (medium to large in size, containing flattened vesicles, and making symmetrical contacts) are well represented. In general, terminal types I (small, containing densely packed round vesicles/and making asymmetrical contacts) and VI (small and irregular in shape, containing flattened vesicles and making symmetrical contacts) are found infrequently. The identity of different types of synaptic terminal is discussed.
Location of motoneurons supplying upper neck muscles in the chicken studied by means of horseradish peroxidase
Authors: Watanabe Tohru , Ohmori Yasushige 
Published: January 2005

AbstractThe distribution of motoneurons innervating the upper cervical muscles, biventer cervicis, splenius capitis, complexus, rectus capitis dorsalis, rectus capitis lateralis, and rectus capitis ventralis in the chicken was examined by retrograde transport of horseradish peroxidase. Labeled motoneurons supplying upper neck muscles ranged from 30 to 60 μm in diameter and were located within two subnuclei in the brainstem. The rostrocaudal distributions of motoneurons projecting to individual cervical muscles ranged from 3 to 9 mm in length, both rostral and caudal to the obex. Detailed analysis of the data showed that the more dorsally positioned subnucleus projected mainly to the hypaxial muscles, i.e., the rectus capitis ventralis and lateralis, whereas the ventral subnucleus supplied chiefly the epaxial muscles, i.e., the biventer cervicis and splenius capitis. The complexus and rectus capitis dorsalis were innervated by both of these subnuclei.Historically these dorsal and ventral subnuclei, respectively, have been called the nucleus hypoglossus ventralis and the nucleus hypoglossus ventralis ventrolateralis. In view of the observation that these nuclei do not undergo retrograde degeneration following section of the hypoglossal nerves, this older nomenclature is misleading. In agreement with other authors, we suggest that these motoneuron groups should be collectively referred to as the nucleus supraspinalis.
Serotoninergic system in the brainstem of the marmoset: A combined immunocytochemical and three‐dimensional reconstruction study
Authors: Hornung Jean‐Pierre , Fritschy Jean‐Marc 
Published: January 2005

AbstractThe distribution and morphology of serotoninergic neurons in the marmoset (New‐World monkey) brainstem were studied by immunocytochemistry and computer‐assisted threevdimensional reconstruction. The cytoarchitectonic localization of serotoninergic neurons was ascertained in series of adjacent immunostained and Nissl‐stained sections, and the extent and shape of the serotoninergic nuclei were visualized by computer reconstruction. The overall distribution of the immunoreactive neurons is comparable to that already described for several species of primates. The serotoninergic nuclei are spatially well segregated into an anterior and a posterior group. The anterior group, in the mesencephalon and the rostral pons, contains the largest population of serotoninergic neurons. These neurons are not confined to the raphe nuclei near the midline, but rather expand laterally in the reticular formation. This expanded distribution of the neurons in the anterior group results in a partial fusion of the nuclei. In some nuclei, particularly the median raphe, subdivisions can be clearly delineated on the basis of the distinct morphology of the neurons and of their clustering. The neurons of the posterior group, in the caudal pons and the medulla, are almost all contained within the limits of the raphe nuclei. The serotoninergic neurons located in the reticular formation form a lateral column, which is clearly separated from the serotoninergic neurons found near the midline.Immunoreactive axons are distributed throughout the brainstem, but they innervate certain motor and sensory nuclei more densely. It was consistently found in newborn animals that the overall immunoreactive axonal network was richer than in juveniles or adults, suggesting that there may be a major modification in the function of the serotoninergic system around birth.
Regeneration of adult dorsal root axons into transplants of embryonic spinal cord
Authors: Tessler A. , Himes B. T. , Houle J. , Reier P. J. 
Published: January 2005

AbstractTransplants of the embryonic rat spinal cord survive and differentiate in the spinal cords of adult and newborn host rats. Very little is known about the extent to which these homotopic transplants can provide an environment for regeneration of adult host axons that normally terminate in the spinal cord. We have used horseradish peroxidase injury filling and transganglionic transport methods to determine whether transected dorsal roots regenerate into fetal spinal cord tissue grafted into the spinal cords of adult rats. Additional transplants were examined for the presence of calcitonin gene‐related peptidelike immunoreactivity, which in the normal dorsal horn is derived exclusively from primary afferent axons. Host animals had one side of the L4‐5 spinal cord resected and replaced by a transplant of E14 or E15 spinal cord. Adjacent dorsal roots were sectioned and juxtaposed to the graft. The dorsal roots and their projections into the transplants were then labeled 2–9 months later. The tracing methods that used transport or diffusion of horseradish peroxidase demonstrated that severed host dorsal root axons had regenerated and grown into the transplants. In addition, some donor and host neurons had extended their axons into the periphery to at least the midthigh level as indicated by retrograde labeling following application of tracer to the sciatic nerve. Primary afferent axons immunoreactive for calcitonin gene‐related peptide were among those that regenerated into transplants, and the projections shown by this immunocytochemical method exceeded those demonstrated by the horseradish peroxidase tracing techniques. Growth of the host dorsal roots into transplants indicates that fetal spinal cord tissue permits regeneration of adult axotomized neurons that would otherwise be aborted at the dorsal root/spinal cord junction. This transplantation model should therefore prove useful in studying the enhancement and specificity of the regrowth of axons that normally terminate in the spinal cord.
Reorganization of primary afferent nerve terminals in the spinal dorsal horn of the primate caudal to anterolateral chordotomy
Authors: Bullitt Elizabeth , Stofer William D. , Vierck Charles J. , Perl Edward R. 
Published: January 2005

AbstractA primate model has been used to explore the possibility that anterolateral chordotomy may produce intraspinal sprouting or rearrangement of primary afferent nerve terminations that could account for delayed postoperative recovery of sensory function. Monkeys were trained to limit the duration of an electrical stimulus, and the vigor and frequency of their escape responses were used to differentiate painful from nonpainful levels of stimulation. Behavioral testing after chordotomy revealed: (1) contralateral hypalgesia in all animals, with sensory recovery in half of the group, and (2) bilateral decreases in reflexive force in all animals, with reflex recovery in the majority of monkeys. At the terminal experiment, dorsal rootlets caudal to the spinal lesion were labeled bilaterally with HRP, and the distribution of labeled synaptic complexes was determined within the dorsal horn. When compared to controls, animals undergoing chordotomy showed a loss of terminals in the superficial dorsal horn and an increase of synaptic enlargements in deeper layers. These effects were bilateral, but were most pronounced on the side contralateral to chordotomy. Animals with diffuse spinal lesions showed a completely different change in the distribution of primary afferent terminals. Animals with sensory recovery demonstrated a more normal terminal distribution pattern than persistently hypalgesic monkeys, but there was considerable variability in the data, and analysis by different statistical tests yielded varying results.
FMRFamide‐ and adipokinetic hormone‐like immunoreactivity in the nervous system of the mosquito, Aedes aegypti
Authors: Brown Mark R. , Lea Arden O. 
Published: January 2005

AbstractAs demonstrated with immunocytochemistry, specific cells and axons in the nervous system of female Aedes aegypti contain antigens immunologically related to FMRFamide (phenylalanine‐methioninevarginine‐phenylalaninevamide) and locust adipokinetic hormone I (AKH). In the supravesophageal ganglion, including some medial neurosecretory cells, and in all ganglia of the ventral nerve cord, there are 100–120 cells immunoreactive to a FMRFamide antiserum. The same cells cross‐react with a bovine pancreatic polypeptide antiserum, but when the latter antiserum is preabsorbed with FMRFamide, immunoreactivity is lost. However, immunoreactivity is maintained when FMRFamide antiserum is preabsorbed with pancreatic polypeptide, suggesting that the immunoreactive peptide is more closely related to FMRFamide.There are 6–12 cells in the supra‐ and subesophageal ganglia immunoreactive to an AKH antiserum, and some of the same cells are reactive to the FMRFamide antiserum. As well, unpaired cells in each of the abdominal ganglia are positive for both AKH and FMRFamide.Although the function of the FMRFamidev and AKH‐like peptides in mosquitoes is unknown, this study, combined with previous reports on the localization of FMRFamidevlike peptides in midgut endocrine cells, supports the concept of a brain‐midgut neuroendocrine axis in this insect.