bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beyond the norm: A rare case of cardiac and extracardiac metastatic urothelial carcinoma
Authors: Qavi Ahmed Hassaan , Fakhar Tehniat , Khalid Mahnoor , El Sharu Husam , Ahmad Soban , Shammas Rony , Marcu Constantin Bogdan 
Published: October 2023

Key Clinical MessageScreening echocardiography aids in identifying cardiac emboli causes and asymptomatic cardiac metastases in high‐grade neoplasms. Conversely, cardiac MRI provides advanced tissue characterization and broader extracardiac assessment.
gem‐Difluoroolefination of Amides
Authors: Trifonov Alexey L. , Dilman Alexander D. 
Published: October 2023

AbstractA metal‐free one‐pot process for the gem‐difluoroolefination of amides is described. The reaction is based on interaction of generated in situ α‐chloroiminium salts with difluorinated phosphorus ylide formed from difluorocarbene and triphenylphosphine. The olefination involves nucleophile‐assisted dephosphorylation and proceeds within one hour at low temperature. The gem‐difluoroenamines were used in further transformations leading to a variety of fluoroalkylated amines.
Latrunculin resistance mechanism of non‐conventional actin NAP1 uncovered by molecular dynamics simulations
Authors: Morita Rikuri , Shigeta Yasuteru , Harada Ryuhei 
Published: October 2023

AbstractMonomeric G‐actin polymerizes into F‐actin to perform various cellular functions. Actin depolymerization drugs, such as latrunculin‐A (Lat‐A), inhibit filament formation and disrupt the cytoskeleton. Interestingly, the green algae Chlamydomonas alternatively produces a non‐conventional actin, NAP1, that responds to inhibition by latrunculin. However, the molecular mechanism underlying latrunculin resistance of NAP1 remains unclear because of the difficulty due to its low in vitro polymerizability. Instead of biochemical experiments, we performed molecular dynamics (MD) simulations to investigate whether NAP1 has a lower affinity for Lat‐A than the conventional actins. Our phylogenetic comparison of the binding free energies shows that Lat‐A is evolutionarily optimized for skeletal muscles. By decomposing the binding free energy into each amino acid residue, we found that some residues in NAP1 play an important role in latrunculin resistance, suggesting that the primary mechanism of latrunculin resistance is the loss of affinity for Lat‐A due to substitutions. In conclusion, our binding‐free‐energy calculations using MD simulations provide the critical insight that loss of affinity is the direct mechanism of latrunculin resistance.
Design of Balanced Cyclooxygenase Inhibitors Based on Natural Anti‐inflammatory Ascidian Metabolites and Celecoxib
Authors: Fang Jingjie , Shang Ziyi , Kaliaperumal Kumaravel , Ju Zhiran , Chen Fen‐Er 
Published: October 2023

AbstractThe serious adverse effects caused by non‐selective and selective cyclooxygenase‐2 (COX‐2) inhibitors remain significant concerns for current anti‐inflammatory drugs. In this study, we present the design and synthesis of a novel series of celecoxib analogs incorporating a hydrazone linker, which were subjected toin silicoanalysis to compare their binding poses with those of clinically used nonsteroidal anti‐inflammatory drugs (NSAIDs) against COX‐1 and COX‐2. The synthesized analogs were evaluated for their inhibitory activity against both COX enzymes, and compound6 m, exhibiting potent balanced inhibition, was selected for subsequentin vitroanti‐inflammatory assays. Treatment with6 meffectively suppressed the NF‐κB signaling pathway in lipopolysaccharide (LPS)‐stimulated murine RAW264.7 macrophages, resulting in reduced expression of pro‐inflammatory factors such as inducible nitric oxide synthase (iNOS), COX‐2, tumor necrosis factor‐α(TNF‐α), interleukin‐6 (IL‐6), IL‐1β, as well as decreased production of prostaglandin E2(PGE2), nitric oxide (NO), and reactive oxygen species (ROS). However,6 mhas no effect on the MAPK signaling pathway. Therefore, due to its potentin vitroanti‐inflammatory activity coupled with lack of cytotoxicity,6 mrepresents a promising candidate for further development as a new lead compound targeting inflammation.
Methane Activation by [AlFeO3]+: the Hidden Spin Selectivity
Authors: Yan Linghui , Yuan BoWei , Qian Chao , Zhou Shaodong 
Published: October 2023

AbstractThe performance of heteronuclear cluster [AlFeO3]+ in activating methane has been explored by a combination of high‐level quantum chemical calculations with gas‐phase experiments. At room temperature, [AlFeO3]+ is a mixture of 7[AlFeO3]+ and 5[AlFeO3]+, in which two states lead to different reactivity and chemoselectivity for methane activation. While hydrogen extracted from methane is the only product channel for the 7[AlFeO3]+/CH4 couple, 5[AlFeO3]+ is able to convert this substrate to formaldehyde. In addition, the introduction of an external electric field may regulate the reactivity and product selectivity. The interesting doping effect of Fe and the associated electronic origins are discussed, which may guide one on the design of Fe‐involved catalyst for methane conversion.
Epoxide/CO2 Cycloaddition Reaction Catalyzed by Indium Chloride Complexes Supported by Constrained Inden Schiff‐Base Ligands
Authors: Piyawongsiri Thitirat , Laiwattanapaisarn Nattiya , Virachotikul Arnut , Chumsaeng Phongnarin , Phomphrai Khamphee 
Published: October 2023

AbstractCyclic carbonates have received significant interests for uses as reagents, solvents, and monomers. The coupling reaction of epoxides with carbon dioxide (CO2) to produce cyclic carbonate is an attractive route which can significantly reduce greenhouse gas emissions and environmental hazards. Herein, a series of five indium chloride complexes supported by inden Schiff‐base ligands were reported along with four X‐ray crystal structures. The constrained five‐membered rings were added to the ligands to enhance the coordination of epoxides to the In metal. From the catalyst screening, In inden complex having tert‐butyl substituents and propylene backbone in combination with tetrabutylammonium bromide (TBAB) exhibited the highest catalytic activity (TON up to 1017) for propylene oxide/CO2 coupling reaction with >99 % selectivity for cyclic carbonate under solvent‐free conditions. In addition, the catalyst was shown to be active at atmospheric pressure of CO2 at room temperature. The catalyst system can be applied to various internal and terminal epoxide substrates to exclusively produce the corresponding cyclic carbonates.
Experimental investigation of effect of ventilation strategies on the performance of cold storage
Authors: Bishnoi Rajkumar , Aharwal K. R. 
Published: October 2023

AbstractFresh fruits and vegetables are living organisms that require fresh air to survive while being stored. Respiratory gases can build up and damage produce if there is not enough ventilation during storage. Ventilation is required to prevent an excessive build‐up of respiratory gases and to maintain the freshness of produce. In hot and dry areas, air ventilation of refrigerated storage can have a negative impact on food produce quality and affect storage performance. An indoor experimental study was carried out to investigate the air ventilation of the cold room during various modes of air supply. The energy recovery device (ERD) with and without evaporative cooling arrangement is used in the air supply and the performance enhancement is compared. The ERD reduces the air ventilation load by more than 50% compared to direct ventilation of the cold room and consequently, it reduces the total power consumption of air ventilation. Furthermore, the overall cooling performance of the system is improved by up to 45.7% when the ERDs are used compared to the direct supply of fresh air during air change mode and the use of energy‐saving equipment has also proven improvements in the desired air conditions for food production.
Vascularization inside the epidermis of Neotropical anurans (Nobleobatrachia)
Authors: Ferraro Daiana P. , Pereyra María E. , Barrionuevo J. Sebastián , Quinzio Silvia , Vera Miriam C. , Taboada Carlos , Faivovich Julián , Brunetti Andrés E. 
Published: October 2023

AbstractAnuran skin is a dynamic organ involved in essential functions that strongly correlate with specific morphological traits. Particularly, gas exchange has been associated with epidermal modifications, such as reduced cell layers and increased vascularization. Here, we describe the epidermal morphology and its association with capillary networks in the dorsal skin of 103 Neotropical anurans (Nobleobatrachia) from different ecomorphs and habitats. Additionally, we examined the lateral and ventral skin for a subset of these species. We report intraepidermal capillaries in (i) dorsal skin of Lepidobatrachus laevis and Lepidobatrachus llanensis (burrowing and semi‐aquatic Chacoan species), Hyloscirtus colymba and Hyloscirtus palmeri (arboreal species from humid forests), and Alsodes neuquensis and 15 Telmatobius spp. (aquatic and semi‐aquatic species from cold environments); (ii) lateral skin of Boana benitezi and H. colymba (arboreal species from humid forests), and (iii) ventral skin of B. benitezi, H. colymba, Atelognathus patagonicus (aquatic species from cold environments), and four Chacoan species, Chacophrys pierottii, Ceratophrys cranwelli (burrowing/terrestrial species), and Lepidobatrachus asper and L. llanensis (burrowing/semi‐aquatic species). Also, verrucae hydrophilicae were observed exclusively in the ventral skin of Leptodactylus fuscus, Leptodactylus laticeps (terrestrial and Chacoan species), and B. benitezi. Regardless of the skin region, the capillaries always penetrate the epidermis from the dermis, while epidermal cell layers are flattened. Our findings support previous hypotheses stating that the environment where species occur influences skin changes related to cutaneous respiration (intraepidermal capillaries in different body regions) and water absorption (intraepidermal capillaries associated with verrucae hydrophilicae within ventral skin). Also, phylogeny might influence the development of these structures, as revealed by the presence of intraepidermal capillaries in almost all analyzed species of Telmatobius. Finally, the co‐occurrence of verrucae hydrophilicae in the ventral skin of hylids from humid forests, and leptodactylids from the subhumid Chacoan region suggest an independent origin.
Manufacturers' incentive to establish direct‐to‐consumer channels
Authors: Lee Dong Joon , Ono Yuji 
Published: October 2023

AbstractIn this paper, we consider a manufacturing duopoly in which each manufacturer initially sells a homogenous product through its dedicated retailer. The main purpose of this paper is to examine whether each manufacturer will endogenously sell its product directly to consumers. Our main results are two. One is that one manufacturer chooses a direct distribution while the other does not. Conventional wisdom suggests that increased market competition is generally presumed to decrease profits. Here, “increased competition” could mean entry of new firms or softening of product differentiation. The other is that the profits of manufacturers are higher under a dual channel than under an indirect channel when the retailing costs of manufacturers are sufficiently small.
Self‐healing thermoplastic elastomeric blends of zinc‐ionomer and styrene–butadiene–styrene block copolymer and their characterization
Authors: Tiwari Shilpi , Bag Dibyendu S , Dwivedi Mayank 
Published: October 2023

AbstractWe report the development of novel self‐healing binary thermoplastic elastomeric blends of Zn2+‐neutralized poly[ethylene‐co‐(methacrylic acid)] (Zn‐ionomer) and styrene–butadiene–styrene block copolymer (SBS). Zn‐ionomer/SBS blends were prepared by the melt‐blending technique using a twin‐screw extruder with three mass ratios of 75/25, 50/50 and 25/75. Structure, phase morphology and thermal as well as tensile properties of the samples were characterized. Fourier transform infrared spectroscopy results indicated the presence of two types of polymeric materials and their interactions. Scanning electron microscopy images confirmed the miscibility of blend components which remarkably influenced the properties of the blends. The optimum peak degradation temperature of Zn‐ionomer/SBS (75/25 wt%) blend was observed to be 484 °C. The ratio of the tensile strength of the healed specimens to that of the original specimens was implemented to quantitatively evaluate the self‐healing efficiency of the materials. The self‐healing efficiencies were calculated to be 55.42%, 58.36% and 60.75% for Zn‐ionomer samples healed for 4, 8 and 16 h, respectively. All of the blend samples exhibited self‐healing behavior and the self‐healing efficiency was more than that of virgin Zn‐ionomer which may be credited to the high mobility of SBS segments in the blends. The healing efficiency was increased with an increase of SBS content in the blends and also heat energy facilitated the healing process. The self‐healing efficiencies of Zn‐ionomer/SBS (25/75 wt%) blend sample were calculated to be 75.09%, 80.27% and 80.87%, respectively, for 4, 8 and 16 h of healing time at 100 °C. A comparison of optical micrographs of the cut area of samples (before healing and after the healing process) was also made and self‐healing of the blend materials was observed. © 2023 Society of Industrial Chemistry.
Efficient integration of electrocoagulation treatment with the spray‐pyrolyzed activated carbon coating on stainless steel electrodes for textile effluent‐bath reuse with ease
Authors: Gowthaman S. , Selvaraju T. 
Published: October 2023

AbstractIn this study, the electrocoagulation (EC) treatment was used to minimize and separate pollutants from textile industrial wastewater (TIWW), including high color, chemical oxygen demand (COD), total organic carbon (TOC), and total dissolved solids (TDS). To enhance the EC treatment efficiency, a novel strategy has been followed in the study that involves thin‐film coating on 316 stainless steel (SS) electrodes with banana peel‐derived activated carbon (BPAC) by dip coating, spin coating, or spray coating. Among the different types of coating, thickness and contact angle measurements have elucidated that the spray coating of BPAC on SS electrode is the best tool with minimum thickness and contact angle. In this study, a bare SS electrode was used as the anode and a thin‐film spray‐coated BPAC on the SS electrode was used as the cathode. Moreover, optimization plays a key role in EC treatment process, where operating conditions such as a current density of 10 mA/cm2, contact time of 15 min, and a pH of 7 were fixed. As a result, the findings indicate comparatively high color removal of 98%, COD removal of 91%, TOC removal of 89.6%, and TDS removal of 68% are achieved with ease. Accordingly, in comparison with plain SS electrodes or dip‐ or spin‐coated BPAC on SS electrodes, spray‐coated BPAC on SS electrodes in EC treatment outperforms in removing high color, TOC, COD, and TDS. Overall, the study highlights the potential of EC treatment integrated with adsorption procedures for TIWW treatment. Particularly, the use of thin‐film spray‐coated BPAC on SS electrodes in the EC treatment process led to an effective and sustainable tool for treating and reuse of TIWW. It is due to its low operation and maintenance cost and studied in a short interval of time. Finally, the ultimate goal was firmly achieved in pilot‐scale studies by the safe discharge into the environment or reuse of treated textile wastewater. Thus, it is a promising alternative with an environmentally friendly footprint that could be easily implemented in any textile industry premises.Practitioner Points
Heavy metals, oils, facts, suspended solids, and other pollutants can be removed from industrial effluent by using electrocoagulation.
The process is both cost‐effective and energy‐efficient, and it is easily integrated with other water treatment technologies.
According to the findings of this study, minimum current density should be applied to BPAC–SS‐coated electrodes by DC power supplies to treat textile industry effluents at low operating costs.
When compared with a plain SS electrode, the spray‐coated BPAC on SS electrode provides better performance in effluent treatment.
Disabled-2: a protein up-regulated by high molecular weight hyaluronan has both tumor promoting and tumor suppressor roles in ovarian cancer
Authors: Price Zoe K. , Lokman Noor A. , Sugiyama Mai , Koya Yoshihiro , Yoshihara Masato , Oehler Martin K. , Kajiyama Hiroaki , Ricciardelli Carmela 
Published: October 2023

AbstractAlthough the pro-tumorigenic functions of hyaluronan (HA) are well documented there is limited information on the effects and targets of different molecular weight HA. Here, we investigated the effects of 27 kDa, 183 kDa and 1000 kDa HA on ES-2 ovarian cancer cells overexpressing the stem cell associated protein, Notch3. 1000 kDA HA promoted spheroid formation in ES-2 cells mixed with ES-2 overexpressing Notch3 (1:3). We report disabled-2 (DAB2) as a novel protein regulated by 1000 kDa HA and further investigated its role in ovarian cancer. DAB2 was downregulated in ovarian cancer compared to normal tissues but increased in metastatic ovarian tumors compared to primary tumors. High DAB2 expression was associated with poor patient outcome and positively correlated with HA synthesis enzyme HAS2, HA receptor CD44 and EMT and macrophage markers. Stromal DAB2 immunostaining was significantly increased in matched ovarian cancer tissues at relapse compared to diagnosis and associated with reduced survival. The proportion of DAB2 positive macrophages was significantly increased in metastatic ovarian cancer tissues compared to primary cancers. However, DAB2 overexpression significantly reduced invasion by both A2780 and OVCAR3 cells in vivo. Our research identifies a novel relationship between HA signalling, Notch3 and DAB2. We highlight a complex relationship of both pro-tumorigenic and tumor suppressive functions of DAB2 in ovarian cancer. Our findings highlight that DAB2 has a direct tumor suppressive role on ovarian cancer cells. The pro-tumorigenic role of DAB2 may be mediated by tumour associated macrophages and requires further investigation.
Evaluierung und Optimierung des Neugeborenenscreenings mittels strukturierter Langzeitbeobachtung – am Beispiel der angeborenen Stoffwechselerkrankungen
Authors: Mütze Ulrike , Kölker Stefan 
Published: October 2023

ZusammenfassungDas Neugeborenenscreening (NGS) ist ein hoch erfolgreiches Programm der Sekundärprävention mit dem Ziel, schwere Folgeschäden von angeborenen, meist genetisch bedingten Erkrankungen durch möglichst frühe präsymptomatische Identifizierung zu verhindern. Bisherige Studien zeigen wichtige Errungenschaften von NGS-Programmen, decken aber auch eine Reihe von relevanten Schwächen auf. Dazu gehören der zumeist unvollständig verstandene natürliche Verlauf und die phänotypische Vielfalt seltener Krankheiten sowie die unzureichende Möglichkeit einer präzisen, frühen Vorhersage des individuellen Krankheitsschweregrads und damit die Unsicherheiten bei der Falldefinition, der Risikostratifizierung und der Behandlungsindikation.Vor dem Hintergrund der rasanten Entwicklungen in den genetischen Hochdurchsatztechnologien und der damit verbundenen Möglichkeiten einer zukünftigen erheblichen Ausweitung der NGS-Programme erscheint es überfällig, die strukturierte Langzeitbeobachtung und die hierauf basierende Evaluation des langzeitlichen Gesundheitsnutzens für die im NGS identifizierten Menschen mit seltenen Krankheiten obligatorisch im NGS-Programm zu verankern. Der vorliegende Beitrag erläutert die Bedeutung der Langzeitbeobachtung für die Evaluation und die kontinuierliche Optimierung des NGS. Klinische Langzeitverläufe der im NGS identifizierten Menschen mit angeborenen Stoffwechselkrankheiten werden beispielhaft dargestellt.
Psychometrische Eigenschaften des KoCoN-V
Authors: Schmidt P. , Zernikow B. , Hartenstein-Pinter A. , Wager J. , Bertolini A. , Blankenburg M. , Classen G. , von der Hagen M. , Hamelmann E. , Raffler S. , Schallner J. , Schimmel M. , Springer S. , Wendel E. , Rostasy K. 
Published: October 2023

Zusammenfassung
Hintergrund
Komplex-chronische Krankheiten bei Kindern und Jugendlichen betreffen sehr häufig das Nervensystem. Nicht selten sind die Kinder abhängig von unterschiedlichsten medizinischen Hilfsmitteln bis hin zur Heimbeatmung und sind oft nicht in der Lage, verbal zu kommunizieren. Der Alltag der betroffenen Familien ist geprägt von häufigen Krankenhausaufenthalten sowie der Inanspruchnahme vielfältiger ambulanter Unterstützungsangebote. Trotz des komplexen Versorgungsbedarfs ist die Einschätzung der Eltern zur Versorgungsqualität dieser Kinder und Jugendlichen kaum untersucht.

Ziel
Die vorliegende Studie zielte darauf ab, einen Fragebogen zur Erfassung der Versorgungsqualität von Kindern und Jugendlichen mit komplex-chronisch neurologischen Erkrankungen zu entwickeln und zu validieren.

Methodik
Zur Validierung wurde eine prospektive multizentrische Studie durchgeführt. Die Rekrutierung der Studienteilnehmer:innen erfolgte von März 2022 bis Oktober 2022 in 6 kinderneurologischen Abteilungen verschiedener Kliniken. Der neu entwickelte Fragebogen (KoCoN-Versorgung, KoCoN-V) richtet sich an Eltern von Kindern und Jugendlichen mit komplex-chronisch neurologischen Erkrankungen und besteht aus 17 Items zur Einschätzung der Versorgungsqualität. Für die Validierung des KoCoN‑V wurde im Rahmen einer Hauptkomponentenanalyse eine verlässliche Messgröße ermittelt. Zudem wurden die Messgenauigkeit (interne Konsistenz) des KoCoN‑V berechnet und die Übereinstimmung des KoCoN‑V mit einem globalen Maß zur Zufriedenheit mit der Versorgungsqualität (konvergente Validität) berechnet.

Ergebnisse
Es konnten Daten von insgesamt 342 Patient:innen erfasst werden. Der Fragebogen wurde von den Teilnehmenden in Bezug auf seine Länge, Verständlichkeit und Relevanz der Fragen als angemessen bewertet. Die Analysen zur Validierung ergaben eine einfaktorielle Struktur; im klinischen Alltag ist somit lediglich ein einzelner Summenwert zu bilden, um die Zufriedenheit der Sorgeberechtigten mit der Versorgung ihres chronisch-komplex neurologisch erkrankten Kindes abbilden zu können. Die hohe Korrelation (Spearmans p = −0,678, p < 0,001) der Summenwerte des KoCoN‑V mit dem globalen Maß zur Versorgungsqualität bestätigt die konvergente Validität des Fragebogens. Die Messgenauigkeit (interne Konsistenz) des KoCoN‑V ist exzellent (Cronbachs α = 0,905).

Diskussion
Mit dem KoCoN‑V liegt ein validiertes Maß der elterlichen Bewertung der Versorgungsqualität von Kindern und Jugendlichen mit komplex-chronisch neurologischen Erkrankungen vor.