bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acremonium keratitis in a patient with herpetic neurotrophic corneal disease
Authors: Rodriguez‐Ares Teresa , De Rojas Silva Victoria , Ferreiros Manuel Pereiro , Becerra Eugenio Perez , Tome Carmela Capeans , Sanchez‐Salorio Manuel 
Published: March 2003

ABSTRACT. Fungi belonging to the genus Acremonium Link ex Fries 1821 are ubiquitous environmental contaminants and soil saprophytes, but are infrequent pathogens in humans. These filamentous fungi (previously known as Cephalosporium) are an uncommon cause of mycotic keratitis. As in the case of other filamentous fungi, corneal trauma with contaminated matter is the most frequent risk factor for the infection.We report in this paper a case of keratomycosis caused by Acremonium potronii, in a patient with a history of herpetic keratitis. Medical treatment with amphotericin B was unsuccessful and the infection eventually resolved with penetrating keratoplasty.
Immortalization of human corneal endothelial cells using electroporation protocol optimized for human corneal endothelial and human retinal pigment epithelial cells
Authors: Bednarz Jürgen , Teifel Michael , Friedl Peter , Engelmann Katrin 
Published: March 2003

ABSTRACT.Purpose: In this study we established a protocol for transfection of human corneal endothelial and human retinal pigment epithelial cells. This protocol was used for immortalization of human corneal endothelial cells.Methods: Transfection was performed by means of electroporation. For immortalization a plasmid encoding large and small SV40 T‐antigen was used.Results: The established electroporation protocol was suitable for both cell types. This protocol was used for transfection of human corneal endothelial cells with a plasmid containing the early region of SV40. The transfected cultures exibited an increased life‐span before they entered crisis. One culture recovered from crisis and was cultivated for 300 population doublings. The cells exhibited an in vivo‐like morphology usually lost during cell culture.Conclusions: We describe for the first time a culture of SV40 transfected human corneal endothelial cells which recovered from crisis and can therefore be regarded as immortalized.
Mohs' micrographic surgery for basal cell carcinomas on the eyelids and medial canthal area. II. Reconstruction and follow‐up
Authors: Lindgren Gun , Lindblom Bertil , Larkö Olle 
Published: March 2003

ABSTRACT.Purpose: To report our experience of reconstruction and follow‐up of eyelid basal cell carcinomas treated with Mohs' micrographic technique.Methods: Sixty‐four periocular basal cell carcinomas, with a high risk of recurrence in one or more aspects with regard to location, size, morphology or prior treatment, were excised using Mohs' micrographic technique. All ensuing defects were repaired. The cases were followed prospectively for up to ten years. All complications and interventions were documented during the follow‐up period.Results: The average size of the defect before repair was 21 mm (range 5–45 mm). The mean follow‐up time was 49 months (range 3–120). The recurrence rate was 5% (3 of 64 cases). The three recurrences occurred after one, four and six years, respectively.Conclusions: The recurrence rate of high risk tumours reported in this study was less than reported with other modes of treatment and comparable with that in other studies using Mohs' micrographic technique.
Reliability of computerized perimetric threshold tests as assessed by reliability indices and threshold reproducibility in patients with suspect and manifest glaucoma
Authors: Bengtsson Boel 
Published: March 2003

ABSTRACT.Background: High reproducibility of test measurements is often considered an indication of high reliability of test results. The aim of the current study was to analyse the role of the traditional perimetric reliability indices, False Negative and False Positive responses, and Fixation Losses, as indicators of test reliability in comparison with threshold reproducibility in patients with suspect or manifest glaucoma.Methods: Perimetry was performed in one eye in each of 76 patients. Each eye was tested twice within approximately one week using the Humphrey II 30‐2 S
ita S
tandard program. Frequencies of False Positive and False Negative answers, and rates of Fixation Losses were related to threshold reproducibility and to general field status as expressed by Mean Deviation from age‐normal threshold values using stepwise multiple linear regression analysis.Results: Substantial field loss was associated both with low threshold reproducibility (p<0.0001) and with increased frequency of False Negative answers (p=0.047). The traditional reliability indices contributed marginally compared to amount of field loss when predicting threshold reproducibility; the coefficient of determination decreased non‐significantly from 0.37 to 0.33 when excluding the three reliability indices as explanatory variables from the regression model. Frequencies of False Positive answers and Fixation Losses showed no association to field status or to threshold reproducibility.Conclusion: Reliability of visual field test results in patients with glaucoma expressed as threshold reproducibility, can be predicted by amount of field loss alone, and traditional patient reliability indices contribute surprisingly little in this regard.
The results of cataract extraction and intraocular lens implantation in patients with Behçet's disease
Authors: Süllü Yüksel , Öge İhsan , Erkan Dilek 
Published: March 2003

ABSTRACT.Purpose: To evaluate the results of extracapsular cataract extraction (ECCE) and intraocular lens (IOL) implantation in patients with Behçet's disease.Methods: Retrospective study was based on 19 uveitic eyes of 12 patients with Behçet's disease who underwent classic ECCE with IOL implantation in 5 eyes and phacoemulsification with IOL implantation in 14 eyes between 1993–1999.Results: In eyes with Behçet's uveitis 0.5 or better postoperative visual acuity was found in 21% at the end of mean 34.2±4.6 (range 5 to 66) months follow‐up. The most frequent postoperative complications were posterior capsule opacification in 9 (47%), posterior synechiae in 5 (26%), anterior synechiae in 3 (16%). Anterior and posterior synechiae in the phacoemulsification group were less than those in the ECCE group (respectively p<0.05 and p<0.01).Conclusions: In eyes with chronic Behçet's uveitis, cataract extraction and IOL implantation by phacoemulsification had fewer postoperative complication than ECCE, but most of the patients had low visual acuity due to preoperatively existing optic atrophy and/or inflammatory degeneration or edema of macula.
A child with Muscle‐Eye‐Brain disease
Authors: Fahnehjelm Kristina Teär , Ygge Jan , Engman Mona‐Lisa , Mosskin Mikael , Santavuori Pirkko , Malm Gunilla 
Published: March 2003

ABSTRACT. Purpose: To describe a child with Muscle‐Eye‐Brain disease (MEB), one of three types of congenital muscular dystrophy associated with ocular abnormalities.Methods: Case report.Results: The child showed severe visual impairment due to progressive myopia and retinal degeneration, a pachygyria‐type of migration disorder of the brain with a nodular cortical surface, i.e. cobblestone cortex, as well as muscular weakness and severe mental retardation.Conclusion: Ophthalmological assessments are important to help to diagnose and follow children with congenital muscular dystrophy.
Exfoliation (pseudoexfoliation) syndrome: toward a new understanding
Authors: Ritch Robert , Schlötzer‐Schrehardt Ursula 
Published: March 2003

ABSTRACT.Exfoliation (pseudoexfoliation; PEX) syndrome is the single most common identifiable cause of open‐angle glaucoma, however, very few investigators are engaged in basic research on this disease. To stimulate the field, the First International Think Tank on Exfoliation Syndrome was held in New York in July 1999, comprising clinicians and scientists. This report is a summary of the proceedings of this meeting.
Tear functions in patients with pinguecula
Authors: Oguz Halit , Karadede Sezin , Bitiren Muharrem , Gurler Bulent , Cakmak Mehmet 
Published: March 2003

ABSTRACT. Purpose: To investigate the tear flow, tear film stability and condition of mucus in patients with pinguecula.Methods: The Schirmer I and ferning tests were performed and break‐up time (BUT) was determined in 57 patients with pinguecula [random eyes; 34 males and 23 females, 18 to 70 years of age (mean±SD: 39.1±13.5 years)] and in 57 age‐matched subjects (control) [random eyes; 35 males and 22 females, 11 to 70 years of age (mean±SD: 37.8±15.2 years)], and the test results were statistically analysed.Results: The mean Schirmer test value was 22.72±12 mm in the eyes with pinguecula and 22.7±8.5 mm in the control eyes. There was no statistically significant difference (t=0.01, p=0.99) between the groups. The mean BUTs were 11.42±6.89 s in the eyes with pinguecula and 15.46±5.85 s in the control eyes. Comparison of BUTs between the groups revealed a statistically significant difference (t=3.37, p=0.0010). Also, ferning types were found to be significantly abnormal in the eyes with pinguecula (p<0.01).Conclusion: The alteration in the mucin layer is a possible change inducing pinguecula formation, or it may reflect an already existing pathology in the cells on the ocular surface.
Autologous blood injection for marked overfiltration early after trabeculectomy with mitomycin C
Authors: Okada Koji , Tsukamoto Hidetoshi , Masumoto Makiko , Jian Koji , Okada Mayumi , Mochizuki Hideki , Mishima Hiromu K. 
Published: March 2003

ABSTRACT. Purpose: After trabeculectomy with mitomycin C, extremely low intraocular pressure (IOP) with excess filtration may cause hypotonous maculopathy in the early postoperative period. We evaluated the effect of injecting autologous blood on reversing early postoperative marked hypotony after trabeculectomy with mitomycin C.Methods: Trabeculectomy with mitomycin C was performed in 258 eyes between 1994 and 1998. Peribleb autologous blood injection was performed in five eyes in which pressure patches were ineffective in reversing excess filtration. Approximately 0.1 to 0.3 ml of whole unclotted blood was slowly injected at least 3 mm from the edge of the flap using a sterile 27‐gauge needle.Results: None of these eyes developed hypotonous maculopathy after injection. After a mean 31‐month follow‐up, all eyes had well‐controlled IOP and visual acuity in three eyes was much improved. Postoperative complications included mild IOP elevation in one eye treated with laser suturelysis, and fibrinous pupillary membrane in one eye.Conclusion: In the early postoperative period, autologous blood injection is effective in reversing excess filtration.
The short‐term effect of latanoprost on intraocular pressure and pulsatile ocular blood flow
Authors: Georgopoulos Gerasimos T. , Diestelhorst Michael , Fisher Robert , Ruokonen Peter , Krieglstein Guenter K. 
Published: March 2003

ABSTRACT.Purpose:  There is evidence that ocular blood flow plays a critical role in the clinical course of glaucoma. Any reduction in ocular blood flow due to topical antiglaucoma treatment should therefore be avoided. This study aimed to evaluate the short‐term effect of local latanoprost application on ocular hemodynamics.Methods:  Intraocular pressure (IOP), ocular pulse amplitude (OPA), ocular pulse volume (OPV), systemic blood pressure, heart rate and the pulsatile component of ocular blood flow (POBF) were recorded using a pneumotonometer linked to the Langham Ocular Blood Flow System in 24 patients in a prospective, open‐label study before and after 1 week of topical latanoprost application in both eyes. Twenty of the subjects had primary open‐angle glaucoma and four had ocular hypertension.Results:  After 1 week of latanoprost treatment, IOP decreased significantly 6.2 ± 2.9 mmHg in OD (P < 0.001) and 6.2 ± 3.2 mmHg in OS (P < 0.001). Pulsatile OBF increased significantly by 201.2 ± 167.4 µL/min in OD (P < 0.001) and 203.8 ± 187.3 µL/min in OS (P < 0.001). Ocular pulse amplitude and OPV showed statistically significant increases (P < 0.05 and P < 0.001 respectively). Blood pressure and heart rate did not change significantly.Conclusion:  Our results indicate that 1 week after latanoprost application, POBF, OPA and OPV were significantly increased in the eyes treated. More information on the perfusion of the optic nerve head is needed before the relevance of these findings to optic nerve head blood flow can be interpreted correctly.
Orbital intramuscular haemangioma
Authors: Christensen Steen Rugaard , Børgesen Svend Erik , Heegaard Steffen , Prause Jan Ulrik 
Published: March 2003

ABSTRACT.Purpose:  To present the clinical and histopathological characteristics of an intramuscular haemangioma in the extraocular muscles.Methods:  In 1989, an otherwise healthy 21‐year‐old female developed proptosis of the left eye. Clinical examination revealed a painless, non‐compressible, retrobulbar lesion. No discoloration of skin or conjunctiva could be observed. The patient had no history of head injury. She underwent surgery three times over a period of 9 years, without reaching a diagnosis. The tumour increased slowly in size despite the operations and MR imaging suggested a tumour of angiomatous origin. Finally, radical surgery was performed with enucleation of the left eye and excision of affected extraocular muscles.Results:  Histopathological examination of the removed orbital tumour showed a mixed type intramuscular haemangioma involving four extraocular muscles.Conclusion:  We report the first case of an intramuscular haemangioma of the orbit.
Analysis of mean retinal transit time from fluorescein angiography in human eyes: normal values and reproducibility
Authors: Bjärnhall G. , Mäepea O. , Sperber G. O. , Lindén C. , Mönestam E. , Alm A. 
Published: March 2003

ABSTRACT.Objective:  To evaluate three different techniques to quantify retinal blood flow transit times in normal human eyes from fluorescein angiograms.Subjects and Methods:  Fluorescein angiograms were recorded on two different occasions in 18 normal individuals with a scanning laser ophthalmoscope. The angiograms were digitized (5 frames per second) and the images were aligned. Mean transit times (MTT) were analysed with a newly developed technique based on an impulse‐response analysis (MTTIR) and again with the conventional technique (MTTSLOPE). Arterio‐venous passage times (AVP) were also calculated.Results:  At the first determination, mean values (SD) for MTTIR, MTTSLOPE, and AVP were 3.22 (0.78), 4.88 (1.86), and 1.46 (0.57) seconds, respectively. Detection of an increase of 25% with a power of 80% requires groups of 12, 86 and 17 individuals for the three techniques, respectively.Conclusions:  Mean transit time is a well‐defined physiological parameter. The technique based on impulse‐response analysis allows for analysis of even badly defined dye curves. We found this technique to be superior to the conventional technique in terms of reproducibility.
Retinal arteriolar obstruction with progestogen treatment of threatened abortion
Authors: Lanzetta Paolo , Crovato Sabrina , Pirracchio Angelo , Bandello Francesco 
Published: March 2003

ABSTRACT.Purpose:  To describe a case of a retinal arteriolar occlusion associated with progestogen treatment of threatened abortion.Methods:  Case report.Results:  A 30‐year‐old woman who had received a 2‐week course of high dose intramuscular progestogen for a threatened abortion presented with a paracentral scotoma in her right eye and occlusion of a small arteriole adjacent to the macula. There were no major subsequent variations in haemostasis and pre‐existing systemic diseases were excluded.Conclusions:  Retinal arteriolar occlusion may be associated with high dose progestogen‐only therapy in pregnant women.
The macular thickness and volume in glaucoma: an analysis in normal and glaucomatous eyes using OCT
Authors: Giovannini A. , Amato G. , Mariotti C. 
Published: March 2003

SummaryPurpose:  To evaluate macular volume in normal and glaucomatous eyes using Optical Coherence Tomography (OCT).Methods:  Fifty eyes of 30 patients (age: 49–68); 20 eyes normal, 15 eyes with early glaucoma and 15 eyes with advanced glaucoma have been studied with the commercially available OCT unit (OCT 2000) (Humphrey Zeiss, Dublin, CA, USA). All eyes were examined at a scan length of 3.44 mm vertically across the fovea. OCT macular retinal thickness maps were used to calculate macular volume.Results:  We observed significant differences between groups. Normals (7.35 ± 0.455 mm3) and early glaucoma (7.09 ± 0.475 mm3), each had significantly greater volume than subjects with advanced glaucoma (6.678 ± 0.455 mm3).Conclusions:  Volumetric analysis of macular thickness with OCT tomograms may be a useful method of documenting and monitoring patients with early glaucoma and advanced glaucoma. In our analysis, and according to the observations of other authors, OCT macular volumes correlates significantly with glaucoma status.
On the influence of superstructure connection on implant preload: A methodological and clinical study
Authors: Smedberg J.‐I. , Nilner K. , Rangert B. , Svensson S. A. , Glantz P.‐O. 
Published: March 2003

In a combined in vitro and in vivo study strain gauges were used to register the deformation in two terminal implant abutment cylinders, when connecting three different prosthetic appliances. The prosthetic constructions were placed on the abutments and fastened both in vitro and in vivo by using different torque sequences. Furthermore, in the in vitro situation the three medial abutments were replaced by abutments which were 100 μm shorter.The results show that even if the prosthetic constructions were judged to have a good clinical fit, relatively large External Preloads were registered both in vitro and in vivo. No significant differences in axial force or in bending moment were found between the torque sequences in vivo. There were, however, significant differences in axial force as well as in bending moment in the in vitro experiment. When the shorter medial abutments were used in vitro, a change in the axial force of approximately 200N was registered. The results confirm that the methodological approach used in this study is valid when evaluating preloads.
Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model
Authors: Tresguerres Isabel F. , Clemente Celia , Donado Manuel , Gómez‐Pellico Luis , Blanco Luis , Alobera Miguel Angel , Tresguerres Jesús A. F. 
Published: March 2003

Abstract:  Objective: The purpose of this study was to determine whether rhGH administered locally during the surgical placement of an implant, in the tibia of an osteoporotic rabbit model, would induce qualitative and quantitative differences in peri‐implant bone reaction. Material and methods: Eight New Zealand rabbits were ovariectomized and fed with a low‐calcium diet (with 0.07% of calcium) to induce osteoporosis. After 6 weeks, an experimental titanium sheet was inserted into the rabbit tibiae. The rabbits were randomly divided into two groups. The experimental group was treated with 4 IU of rhGH added locally into the ostectomy as a lyophilized powder and the control group was left without any treatment. After 2 weeks, animals were sacrificed, tibial sections were prepared and bone–titanium interface was examined at light microscopy, using Masson, hematoxylin‐eosin and Pichrosirius stains. Light microscopic morphometry and densitometry were used to comparatively quantitate bone reaction. Results/Conclusion: Local administration of rhGH during the surgical placement of titanium sheets on the tibiae of an osteoporotic rabbit model enhances periosteal and transcortical reaction and mineralization of osteoid 14 days later around titanium sheets, without increasing bone resorption.
Periodontal flap surgery with 25% metronidazole gel
Authors: Needleman Ian G. , Moles David R. , Collins Angela M. 
Published: March 2003

AbstractObjectives:The aim of this study was to examine the effect of periodontal surgery on PGE2 levels in patients treated for advanced adult periodontitis, with and without application of a 25% metronidazole gel over 12 months follow‐up.Material and methods:The trial employed a randomised controlled, single‐blind, parallel arm design on 43 patients with at least 1 pocket probing 6 mm with bleeding on probing (BOP).Results: The results showed that GCF PGE2 levels showed no significant change following surgical therapy despite marked improvements in clinical periodontal health (p=0.653). No significant differences in the reaction of sites to treatment were detected between test and control groups throughout the study (p=0.709). However, PGE2 levels in GCF were significantly higher in experimental sites compared with healthy untreated sites (negative controls) in both surgery only (p=0.001) and surgery+gel (p=0.023) groups throughout the study.Conclusion: Whilst clear differences were observed between healthy control sites and sites with periodontal breakdown, no effect of treatment on PGE2 levels was discernible in this study.
Diamond‐coated sonic tips are more efficient for open debridement of molar furcations
Authors: Auplish Gita , Needleman Ian G. , Moles David R. , Newman Hubert N. 
Published: March 2003

AbstractBackground/aims: The aim of this study was to compare the efficacy of open debridement of class II and III furcations by a new diamond‐coated sonic instrument tip (Sonicparo) with a conventional sonic insert (Sonicrecall) and hand instruments (Gracey curettes).Method: Open debridement was simulated using a manikin head and standardised plastic teeth. The efficacy of debridement was examined by 3 parameters: residual root surface marker, ‘tooth’ weight loss and time taken for debridement. The teeth used were 1st and 2nd upper and lower molars. Upper left and lower right molars were chosen to represent class II furcation involvements and upper right and lower left molars class III furcation involvements. Each tooth was debrided 4 times with each instrument for both class of furcations.Results: The results of this study showed significantly less residual marker area comparing Sonicparo with Gracey curettes (mean difference 2.52 mm2, p<0.001). However, no significant differences in marker removal were observed between Sonicrecall inserts and the other instruments. Sonicparo inserts also took significantly less time to debride the furcation than Gracey curettes (mean difference 98.3 s, p<0.001) or Sonicrecall instruments (mean difference 74.3 s, p<0.001). However no significant differences were found between instruments concerning weight loss of the plastic teeth.Conclusions: It was concluded from this in‐vitro study that the Sonicparo insert was a more efficient instrument for open molar furcation debridement than Sonicrecall and hand instruments (Gracey curettes) when considering both marker removal and debridement time.