bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and genetic diversity of porcine circovirus type 2 in Henan Province of China during 2020-2023
Authors: Peng Zhifeng , Lv Huifang , Zhang Han , Zhao Li , Du Jimei , He Yanyu , Zhao Kangdi , Xu Qianqian , Qiao Hongxing , Bian Chuanzhou 
Published: February 2024

Abstract
The genetic diversity and widespread of porcine circovirus type 2 (PCV2), mainly lead to porcine circovirus-associated diseases (PCVADs), seriously threatens the swine industry worldwide. In the present study, to investigate the recent prevalence and genetic diversity of PCV2 in Henan Province of China, a total of 385 tissue samples collected from pig farms of different sizes during 2020 and 2023 were analyzed by PCR. Overall, 71.17% (274/385) tissue samples of pigs were positive for PCV2, with the detection rates of PCV2 were 81.16% (112/138), 72.41% (84/116), 62.50% (55/88), 53.49% (23/43) in 2020, 2021, 2022, 2023, respectively, indicating the detection rates of PCV2 decreased progressively in Henan Province of China in recent years. Additionally, the complete genomes of 34 PCV2 strains were sequenced and analyzed, which indicated that their nucleotide identities were 93.9–100.0%, 96.70–100.0%, and 88.90–100.0% in the complete genome, ORF1, ORF2, respectively, and amino acid identities were 98.10–100.0% and 88.60–100.0% in the replicase and capsid proteins, respectively. Phylogenetic analysis based on the complete genome sequences and deduced amino acids of ORF2 showed that the PCV2 strains obtained in the present study could be classified into three sub-clades, with the most strains clustered into clade PCV2d, indicated PCV2d is the predominated subtype in Henan Province, China. Moreover, four substitution sites (V57I, I89R/L, P134T, D210E) were observed in PCV2b ORF2 that were absent in PCV2a and PCV2d, and 4 substitution sites (S68N, I89L, T/P134N, S169G/R) were observed in PCV2d ORF2 that were absent in PCV2a and PCV2b. In general, the present study revealed a high prevalence and high genetic divergence of PCV2 among Chinese swine herds, provided an insight into the ongoing emerged PCV2, and enriched our understanding of the genetic diversity and evolution of PCV2.
Spatial transcriptomics reveals substantial heterogeneity in triple negative breast cancer with potential clinical implications
Authors: Wang Xiaoxiao , Venet David , Lifrange Frédéric , Larsimont Denis , Rediti Mattia , Stenbeck Linnea , Dupont Floriane , Rouas Ghizlane , Garcia Andrea Joaquin , Craciun Ligia , Buisseret Laurence , Ignatiadis Michail , Bhalla Nayanika , Masarapu Yuvarani , Villacampa Eva Gracia , Franzén Lovisa , Saarenpää Sami , Kvastad Linda , Thrane Kim , Lundeberg Joakim , Rothé Françoise , Sotiriou Christos 
Published: February 2024

Abstract

While triple negative breast cancer (TNBC) is known to be heterogeneous at the genomic and transcriptomic levels, spatial information on tumor organization and cell composition is still lacking. Here, we investigated TNBC tumor architecture including its microenvironment using spatial transcriptomics on a series of 92 patients. We performed an in-depth characterization of tumor and stroma organization and composition using an integrative approach combining histomorphological and spatial transcriptomics. Furthermore, a detailed molecular characterization of tertiary lymphoid structures led to identify a gene signature strongly associated to disease outcome and response to immunotherapy in several tumor types beyond TNBC. A stepwise clustering analysis identified nine TNBC ecotypes, further validated in external datasets. Several ecotypes were associated with disease outcome and characterized by potentially actionable features. This is the largest TNBC series providing a comprehensive insight into the complexity of TNBC ecosystem with potential clinical relevance, opening avenues for treatment tailoring including immunotherapy.
Comparison of Resuscitation Intervention Utilization in the Emergency Department by Palliative Care Eligible Patients Between Cancer and Non-Cancer
Authors: Wibulpolprasert Arrug , Wangviboonchai Varaporn , Saengprateeptong Pich , Chongthavonsatit Nichapha , Pongsettakul Natthakorn , Prachanukool Thidathit 
Published: February 2024

Abstract
Background Resuscitation interventions are procedures for managing clinical deterioration in patients with life-threatening conditions by securing the airway, maintaining breathing, or supporting circulation. Little is known about differences in the use of resuscitation interventions and the characteristics of patients eligible for palliative care in the emergency department. The objectives of this study are to compare resuscitation interventions in the emergency department between patients with cancer and non-cancer palliative care eligibility.
Methods A retrospective cohort study was conducted by reviewing the medical records of the palliative care eligible patients who visited the emergency department of one university hospital between January and June 2019.
Results Among the 956 visits (13.7%) by palliative care eligible patients of 7000 random visits, 480 were patients with cancer (50.2%), and 476 were non-cancer patients (49.8%). The overall median age was 72, and 54.1% were female. The mortality rates in the following year were 35.0% for patients with cancer and 18.7% for non-cancer illnesses. The patients with cancer and non-cancer received prior palliative care at 7.3% and 0.8% (p <0.001) and initiated palliative care at the emergency department at 6.3% and 2.7%, respectively (p = 0.008). The non-cancer patients utilized overall resuscitation interventions 1.7 times more than the cancer patients (aOR = 1.7, 95% CI = 1.0–2.6, p = 0.030).
Conclusion For the palliative care eligible patients in the emergency department, patients with cancer and non-cancer significantly differed in their characteristics. Patients with non-cancer received more resuscitation interventions than patients with cancer.
Superior Energy Storage Performance up to 200°C in a Self-organized Trirelaxor-antiferroelectric Nanocomposite
Authors: Gao Jinghui , Xu Jingzhe , Liu Yongbin , Wang Dong , He Li , Zhong Lisheng , Wu Ming , Yao Ruifeng , Zhang Nan , Lou Xiaojie , Li Shengtao , Ren Xiaobing 
Published: February 2024

Abstract

Despite extensive efforts over the past decade in enhancing the energy storage properties of dielectric materials, a challenging issue has remained unsolved that a material with good room-temperature-performance usually degrades significantly at higher temperatures. This issue renders many otherwise promising dielectric energy storage materials unusable because significant temperature rise of the energy storage devices is inevitable during their service and some applications are in high temperature environment. The challenge at high temperatures arises from the physical inevitability of vanishing ferroelectric domains that lead to drop in polarization, and exponentially increased charge transport activity which leads to charge leakage and electrical breakdown. Here we report a material strategy to meet this challenge by designing a self-organized nanocomposite (1-x)(Ba,Sr)(Ti,Sn)O3-xBi1.5ZnNb1.5O7 composed of nano-sized antiferroelectric particles embedded into a trirelaxor matrix through a nanoscale phase separation process. At the optimal composition of x=0.11, the antiferroelectric-trirelaxor nanocomposite ceramic exhibits an outstanding energy storage performance from room temperature (energy density=8.5 J/cm3, efficiency=94.8% and a high figure of merit of 167 J/cm3) up to a high temperature of 200°C (energy density ~4.85 J/cm3, efficiency>90% and figure of merit of 49 J/cm3), which outperforms existing Pb-free dielectric materials. High-resolution transmission electron microscopy (TEM) and synchrotron x-ray diffractometry reveal that the coherent nanometric antiferroelectric particles and the trirelaxor nanodomains sustain over a wide temperature range. In-situ piezoresponse force microscopy (PFM) observation and phase-field simulations show that the nearly hysteresis-free switching of trirelaxor nanodomains is responsible for enhanced polarization (and hence energy density) and low hysteretic loss. Resistivity shows a 2~3 order of magnitude increase in electrical resistivity accompanying significant increase in breakdown strength up to high temperatures. Thermally stimulated depolarization current (TSDC) measurements suggest that the suppression of charge transport or leakage up to high temperature stems from charge trapping effect at high-density nanointerfaces between antiferroelectric and trirelaxor phases. These favorable effects in the nano-composite are responsible for its high energy storage performance up to high temperatures. Our research may provide a generic approach to achieving advanced dielectrics with outstanding performance at high temperatures.
Reliability Inference for Gompertz Distribution Using Pivotal Method under Block Progressive Type-II Censored Data
Authors: Kumari Rani , Tripathi Yogesh Mani , Gangopadhyay Aditi Kar , Lodhi Chandrakant 
Published: February 2024

Abstract
In this paper, we primarily focus on making important deductions regarding the estimates of survival characteristics in the context of block progressive censored data. Specifically, we examine the reliability function, hazard rate function, median time to failure, and variations among different test facilities. Our analysis is centered on the lifetime of test units following the Gompertz distribution. We derive maximum likelihood estimators for unknown quantities and investigate their properties regarding existence and uniqueness. Additionally, we construct approximate confidence intervals for all survival characteristics using the delta method and likelihood theory. Moreover, we develop point and generalized confidence interval estimators through a pivotal quantity-based method. To evaluate the performance of our proposed approaches, we conduct a simulation study and find that the pivotal quantity-based approach yields superior estimation results. Finally, we apply the proposed estimates to analyze real-world data, demonstrating their effectiveness.
The effects of experimental conditions on extraction of polyphenols from African Nutmeg peels using NADESs-UAE: A Multifactorial Modelling Technique
Authors: OKEKE UDODINMA JUDE , Micucci Matteo , Mihaylova Dasha , Cappiello Achile 
Published: February 2024

Abstract

The extraction of polyphenolic compounds from African nutmeg peels via MFAT using NADESs-UAE was investigated to develop sustainable separation to recover natural antioxidants from food waste biomass. In this study, citric acid-based DESs paired with sucrose, fructose, xylitol, glycerol, glycine, and glucose were explored as potential extractants to evaluate the effects of solvent structure on extraction efficiency. Furthermore, the effects of different operating conditions were evaluated comprehensively to determine the parameters necessary for antioxidant recovery in terms of TPC and TFC, and antiradical scavenging activity based on CUPRAC and FRAP methods. The conditions that gave the highest values of 908.17 mg GAE/g DW, 1431.51 µg QE/g DW, 11.5568 µMTE/g DW, and 14.7903 µMTE/g DW for TPC, TFC, CUPRAC, and FRAP are shown in Figures 2D, 3B, 5B, and 6D respectively using CaFr12, CaGc11, CaFr11, CaXy11 as solvents respectively. These results underscore the potential of using specific NADES formulations to tailor the extraction process for desired antioxidant and antiradical scavenging properties. The findings give insights to the capacity of NADESs as powerful solvents for extraction applications. The lack of literature on extraction of bioactive compounds from African nutmeg materials using DESs with ultrasound techniques proves the originality of the present work.
Psychosocial work aspects, work ability, mental health and infection rates of on-site and remote Brazilian workers during the COVID-19 pandemic – a longitudinal study
Authors: Andrade Marcela Alves , Andrews David M. , Sato Tatiana Oliveira 
Published: February 2024

Abstract

Background At the beginning of the COVID-19 pandemic, some workers had the opportunity to work from home, while others remained in on-site work. The aim of the present study was to compare the psychosocial aspects, work ability, mental health conditions and infection rates of Brazilian workers in remote and on-site work through a longitudinal study with quarterly follow-up assessments over a 12-month period.Method A total of 1,211 workers from different economic sectors participated in the study, 897 of whom (74.1%) worked from home and 314 (25.9%) remained in on-site work. Psychosocial aspects were assessed using the Copenhagen Psychosocial Questionnaire (COPSOQ). Work ability was assessed using the Work Ability Index (WAI) and the Work Ability Score (WAS). Mental health conditions and infection rate were recorded based on self-reported medical diagnoses. Online questionnaires were answered from June 2020 to September 2021, involving two waves of the COVID-19 pandemic. The groups were compared using chi-square tests, t-tests, and two-way ANOVA.Results In the first wave of the pandemic, remote workers reported more quantitative demands and work-family conflicts, whereas on-site workers reported more emotional demands, low development of new skills, low commitment, low predictability, low recognition, and low satisfaction. They also reported greater occurrences of unwanted sexual attention, threats of violence, and physical violence. In the second wave, the remote group continued to report high work-family conflicts, whereas the on-site group reported – in addition to the results of the 1st wave – low influence at work, low quality of leadership, and burnout. No significant difference was found between groups with regards to the WAI in either wave (1st wave: P = 0.46; 2nd wave: P = 0.62). Most workers in both groups reported good work ability in both periods. For the WAS, a significant difference was found between the 3rd and 12th months (P < 0.01) in both groups, with a mean reduction of 0.4 points.Conclusions Psychosocial critical aspects differed between groups. Work ability and mental health conditions were similar between remote and on-site workers. The encouragement of remote work could have prevented COVID contamination in the Brazilian population.
WITHDRAWN: Optimization of Accounting Information System in Public sector for Sustainable Risk Management under Big Data Analytics Capabilities. Does Forensic Accountants’ Skill generate differences?
Authors: Pham Quang Huy , Vu Kien Phuc 
Published: February 2024

Abstract
The current manuscript sets its sight to allot an intelligible picture of how to optimize of accounting information system (AIS) in public sector organization (PSO) for sustainability risk management (SRM) under the Big data analytics capabilities (BDAC) and offer in-depth understandings concerning to the role of forensic accountants’ skill (FAS) on these aforementioned interconnections. The structural equation modeling (SEM) and multi-group SEM were wielded to testify the hypothesized model rested on cross-sectional data formulated by a close-ended questionnaire survey distributed to convenience and snowball sample of 583 respondents in PSOs. All of the proffered hypotheses in the theorical model were authenticated by the soundly statistical evidences. The observations of the current study also generated the numerous practical implications for the practitioners in organizational management and policy-makers in building up the strategies and promulgating rules in relation to digital initiatives adoption, accounting practices and risk management toward sustainable development within PSOs.
JEL Classification System: H83, G32
Assessing GERAADA Score Mortality Predictions in Type A Aortic Dissection Patients
Authors: Nampoukime Kan-paatib Barnabo , Monteiro Igwenandji Adeoumi Esperance , Hu Libing , Pan Youmin , Zha Zhengbiao , Nouani Lud Merveil , Gomes Djessica Fortes , Sultani Mustafa Abbas Farhood , Wang Haihao 
Published: February 2024

Abstract

Objective: This study seeks to assess the predictive precision of the GERAADA score for 30-day mortality in patients with Type A aortic dissection.
Methods: A retrospective study analyzed data from 382 survivors and 90 non-survivors, examining demographic, clinical, and surgical variables. GERAADA scores were calculated by a blinded cardiac surgeon using a web-based application.
Results: The overall mortality is 19.06% and 18.18% for GERAADA prediction.
The presence of malperfusion in more than two organs emerged as a significant risk factor for hospital mortality p=0.028. Longer surgery times were significantly associated with elevated mortality p=0.002. Moreover, postoperative ECMO, CPR, and IABP were significantly linked to increased mortality rates. Additionally, ICU stay duration, lung infection, MODS, and respiratory failure p<0.05 independently posed as risk factors for hospital mortality.
Patients with Hemiparesis and peripheral malperfusion experienced no deaths, as predicted by GERAADA score. Additionally, patients with a dissection tear located at the root of the aorta exhibited a lower mortality rate of 7.14%, contrasting the higher 19.87% GERAADA prediction.
Conclusion: GERAADA predictions were mostly accurate, but exceptions occurred with inotrope use, hemiparesis, peripheral malperfusion, and aortic dissection at the root. We suggest enhancing the GERAADA score by incorporating intraoperative and postoperative factors.
Gender differences in skilled performance under failure stress
Authors: Li Chunhua , Zhao Yangqing 
Published: February 2024

Abstract

This study explores the gender differences in archers’ ability to cope with adversity. Using data from professional recurve archery competitions held between 2012 and 2021, the Poisson regression analysis reveals that male and female archers' performance dropped significantly after experiencing two consecutive missing bullseyes, which means a cold-hand effect exists. However, although there was no significant difference in the performance of male and female archers on the third arrow, female archers have significantly lower last arrow per set scores than male archers after near poor performance or being in a situation where losing can only be avoided by winning the current set. This finding suggests that female archers are more vulnerable to the potentially negative effects of adversity caused by trailing or recent failures than their male counterparts. In addition, the reason for such a gender gap has been discussed.
Identification of diagnostic biomarkers for diabetes nephropathy by multi-chip integrated bioinformatics combining machine-learning strategies and mendelian randomization
Authors: Su Jiaming , Guo Yan , Hu Jiyuan , Peng Jing , Dong Zhaoxi , Xu Zheyu , Yu Xinhui , Mei Jie , Wang Lin , Zhang Xianhui , Liu Hongfang 
Published: February 2024

Abstract

Background Diabetic nephropathy (DN) represents a significant complication of diabetes, progressively contributing to the global incidence of end-stage renal disease (ESRD). Currently, the diagnosis of DN primarily relies on traditional clinical indicators, which may not adequately reflect the progression and prognosis of all DN patients, posing challenges in developing diagnostic and therapeutic strategies. This study aims to identify new molecular and genetic biomarkers closely associated with the onset and progression of DN through bioinformatics and genetic epidemiology analysis methods. Methods Gene expression profiles were obtained from gene expression omnibus (GEO) database. The R software was employed to filter differentially expressed genes (DEGs) and to conduct enrichment analyses of these genes. It is remarkable that five distinct machine learning classifiers were utilized to identify diagnostic biomarkers and to formulate a diagnostic model. These biomarkers were further validated in an external validation dataset generated by Nephroseq V5, whereupon a clinical characteristic correlation analysis ensued. In light of the machine learning results, immune infiltration analysis and single-cell RNA sequencing were undertaken. Ultimately, the mendelian randomization method was used to examine the causal relationship between the identified biomarkers and DN. Results Five biomarkers implicated in DN, including AFM, DUSP1, KRT19, TGFBI, and ZFP36, were subjected to external testing and validation, utilizing various machine learning models with high diagnostic efficacy. Correlation analysis demonstrated that the expression of these biomarkers correlated with the deterioration of kidney function, and single-cell RNA sequencing results indicated that the biomarkers were predominantly localized in granulocytes, macrophages, and monocytes. The results from the inverse variance weighted (IVW) analysis elucidated that DUSP1 (OR = 0.664) serves as a protective factor for DN, whereas TGFBI (OR = 1.114) constitutes a risk factor for DN, in agreement with bioinformatics analysis. Conclusions The present study may provide new insights into the mechanisms underlying DN onset and progression, as well as the selection of DN diagnostic markers and therapeutic targets.
Effectiveness of Low-Level Laser Therapy in Reducing Pain Score and Healing Time of Recurrent Aphthous Stomatitis: A Systematic Review and Meta-Analysis
Authors: Radithia Desiana , Mahdani Fatma Yasmin , Bakti Reiska Kumala , Parmadiati Adiastuti Endah , Subarnbhesaj Ajiravudh , Pramitha Selviana Rizky , Pradny I Gusti Agung Sri 
Published: February 2024

Abstract

Background:
Recurrent aphthous stomatitis (RAS) is a common chronic inflammatory oral disease which negatively impacts the quality of life. Current therapies aim to reduce pain and healing process yet challenges such as rapid loss due to salivary flushing in topical drugs and adverse effect due to prolonged use of systemic medications require further notice. Low-level laser therapy is reported with immediate pain relief and faster healing thus preserving potential for optimal treatment modalities. This review critically analyses and summarizes the effectiveness of LLLT in reducing pain score and healing time of RAS.
Methods:
A systematic search was conducted in ScienceDirect, PubMed, and Scopus using keywords of low-level laser therapy, photo-biomodulation therapy, and recurrent aphthous stomatitis. RCTs between 1967 to June 2022, presenting characteristics of laser and reporting pain score and/or healing time of RAS after irradiation were included. Animal studies and recurrent aphthous ulcer with a history of systemic condition were excluded. Studies were critically appraised using RoB 2 tool. A meta-analysis was performed using inverse variance random effects.
Results:
Fourteen trials with a total of 664 patients were included. Reduced pain was reported in 13 studies, while shortened healing time was presented in 4. The pooling of two studies after CO2 irradiation demonstrated faster healing time compared to placebo (MD: -3.72; 95% CI: -4.18, -3.25).
Conclusion:
Pain score and healing time of RAS were reduced after irradiation with LLLT. RoB resulted in “some concerns” urging well-designed RCTs with larger samples to further assess each laser application for comparison.
Systematic review registration: PROSPERO CRD42022355737.