bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphine Preconditions Purkinje Cells against Cell Death under In Vitro Simulated Ischemia–Reperfusion Conditions
Authors: Lim Young Jin , Zheng Shuqiu , Zuo Zhiyi 
Published: February 2004

Background
Morphine pretreatment via activation of delta1-opioid receptors induces cardioprotection. In this study, the authors determined whether morphine preconditioning induces ischemic tolerance in neurons.

Methods
Cerebellar brain slices from adult Sprague-Dawley rats were incubated with morphine at 0.1-10 microM in the presence or absence of various antagonists for 30 min. They were then kept in morphine- and antagonist-free buffer for 30 min before they were subjected to simulated ischemia (oxygen-glucose deprivation) for 20 min. After being recovered in oxygenated artificial cerebrospinal fluid for 5 h, they were fixed for morphologic examination to determine the percentage of undamaged Purkinje cells.

Results
The survival rate of Purkinje cells was significantly higher in slices preconditioned with morphine (> or = 0.3 microM) before the oxygen-glucose deprivation (57 +/- 4% at 0.3 microM morphine) than that of the oxygen-glucose deprivation alone (39 +/- 3%, P < 0.05). This morphine preconditioning-induced neuroprotection was abolished by naloxone, a non-type-selective opioid receptor antagonist, by naltrindole, a selective delta-opioid receptor antagonist, or by 7-benzylidenenaltrexone, a selective delta1-opioid receptor antagonist. However, the effects were not blocked by the mu-, kappa-, or delta2-opioid receptor antagonists, beta-funaltrexamine, nor-binaltorphimine, or naltriben, respectively. Morphine preconditioning-induced neuroprotection was partially blocked by the selective mitochondrial adenosine triphosphate-sensitive potassium channel antagonist, 5-hydroxydecanoate, or the mitochondrial electron transport inhibitor, myxothiazol. None of the inhibitors used in this study alone affected the simulated ischemia-induced neuronal death.

Conclusions
These data suggest that morphine preconditioning is neuroprotective. This neuroprotection may be delta1-opioid receptor dependent and may involve mitochondrial adenosine triphosphate-sensitive potassium channel activation and free radical production. Because morphine is a commonly used analgesic, morphine preconditioning may be explored further for potential clinical use to reduce ischemic brain injury.
Dual Exposure to Sevoflurane Improves Anesthetic Preconditioning in Intact Hearts
Authors: Riess Matthias L. , Kevin Leo G. , Camara Amadou K. S. , Heisner James S. , Stowe David F. 
Published: February 2004

Background
Anesthetic preconditioning (APC) with sevoflurane reduces myocardial ischemia-reperfusion injury. The authors tested whether two brief exposures to sevoflurane would lead to a better preconditioning state than would a single longer exposure and whether dual exposure to a lower (L) concentration of sevoflurane would achieve an outcome similar to that associated with a single exposure to a higher (H) concentration.

Methods
Langendorff-prepared guinea pig hearts were exposed to 0.4 mM sevoflurane once for 15 min (H1-15; n = 8) or 0.4 mM (H2-5; n = 8) or 0.2 mM sevoflurane (L2-5; n = 8) twice for 5 min, with a 5-min washout period interspersed. Sevoflurane was then washed out for 20 min before 30 min of global no-flow ischemia and 120 min of reperfusion. Control hearts (n = 8) were not subjected to APC. Left ventricular pressure was measured isovolumetrically. Ventricular infarct size was determined by tetrazolium staining and cumulative planimetry. Values are expressed as mean +/- SD.

Results
The authors found a better functional return and a lesser percentage of infarction on reperfusion in H2-5 (28 +/- 9%) than in H1-15 (36 +/- 8%; P < 0.05), L2-5 (43 +/- 6%; P < 0.05), or control hearts (52 +/- 7%; P < 0.05).

Conclusion
These results suggest that APC depends not only on the concentration but also on the protocol used for preconditioning. Similarly to ischemic preconditioning, repeated application of the volatile anesthetic seems to be more important than the duration of exposure in initiating the signaling sequence that elicits APC at clinically relevant concentrations. Therefore, repeated cycles of anesthetic exposure followed by volatile anesthetic-free periods may be beneficial for APC in the clinical setting.
Mandibular Advancement Improves the Laryngeal View during Direct Laryngoscopy Performed by Inexperienced Physicians
Authors: Tamura Miki , Ishikawa Teruhiko , Kato Rie , Isono Shiroh , Nishino Takashi 
Published: February 2004

Background
When oral or nasal fiberoptic laryngoscopy is attempted, mandibular advancement has been reported to improve the laryngeal view. The authors hypothesized that mandibular advancement may also improve the laryngeal view during direct laryngoscopy.

Methods
Forty patients undergoing elective surgery under general anesthesia were included in this study. After establishment of an adequate level of anesthesia and muscle relaxation, direct laryngoscopy was performed by inexperienced physicians. Four different maneuvers--simple direct laryngoscopy without any assistance (C), simple direct laryngoscopy with mandibular advancement (M), simple direct laryngoscopy with the BURP maneuver (backward, upward, rightward pressure maneuver of the larynx; B), and simple direct laryngoscopy with both mandibular advancement and the BURP maneuver (BM)--were attempted in each subject, and the laryngeal aperture was videotaped with each procedure. An instructor in anesthesiology who was blinded to the procedure evaluated the visualization by reviewing videotape off-line, using the Cormack-Lehane classification system (grades I-IV) and a rating score within each subject (1 = best view; 4 = poorest view). The Friedman test followed by the Student-Newman-Keuls test was performed for statistical comparison. P < 0.05 was considered significant.

Results
The laryngeal view was improved with M and B when compared with C (P < 0.05 by both rating and Cormack-Lehane evaluation). BM was the most effective method to visualize the laryngeal aperture (P < 0.05, vs. B and M by rating evaluation), whereas B and M were the second and the third most effective methods, respectively. No statistical difference was observed between B and M with the Cormack-Lehane classification.

Conclusion
Mandibular advancement improves the laryngeal view during direct laryngoscopy performed by inexperienced physicians.
ED50and ED95of Intrathecal Hyperbaric Bupivacaine Coadministered with Opioids for Cesarean Delivery
Authors: Ginosar Yehuda , Mirikatani Edward , Drover David R. , Cohen Sheila E. , Riley Edward T. 
Published: February 2004

Background
Successful cesarean delivery anesthesia has been reported with use of small doses (5-9 mg) of intrathecal bupivacaine coadministered with opioids. This double-blind, randomized, dose-ranging study determined the ED50 and ED95 of intrathecal bupivacaine (with adjuvant opioids) for cesarean delivery anesthesia.

Methods
Forty-two parturients undergoing elective cesarean delivery with use of combined spinal-epidural anesthesia received intrathecal hyperbaric bupivacaine in doses of 6, 7, 8, 9, 10, 11, or 12 mg in equal volumes with an added 10 microg intrathecal fentanyl and 200 microg intrathecal morphine. Sensory levels (pinprick) were evaluated every 2 min until a T6 level was achieved. The dose was a success(induction) if a bilateral T6 block occurred in 10 min; otherwise, it was a failure(induction). In addition to being a success(induction), the dose was a success(operation) if no intraoperative epidural supplement was required; otherwise, it was a failure(operation). ED50 and ED95 for both success(induction) and success(operation) were determined with use of a logistic regression model.

Results
ED50 for success(induction) and success(operation) were 6.7 and 7.6 mg, respectively, whereas the ED95 for success(induction) and success(operation) were 11.0 and 11.2 mg. Speed of onset correlated inversely with dose. Although no clear advantage for low doses could be demonstrated (hypotension, nausea, vomiting, pruritus, or maternal satisfaction), this study was underpowered to detect significance in these variables.

Conclusions
The ED95 of intrathecal bupivacaine under the conditions of this study is considerably in excess of the low doses proposed for cesarean delivery in some recent publications. When doses of intrathecal bupivacaine less than the ED95, particularly near the ED50, are used, the doses should be administered as part of a catheter-based technique.
Thoracic and Lumbar Epidural Analgesia via the Caudal Approach Using Electrical Stimulation Guidance in Pediatric Patients
Authors: Tsui Ban C. H. , Wagner Alese , Cave Dominic , Kearney Ramona 
Published: February 2004

Background
Nerve stimulation guidance (Tsui test) has been reported to be an effective alternative to radiographic imaging for proper catheter placement. The purpose of this study was to examine the success rate and complications of continuous caudal epidural analgesia since the implementation of routine use of the Tsui test at the authors' institution.

Methods
The authors examined prospectively collected data in their pediatric pain service database from 289 children who had attempted caudal placement of a lumbar or thoracic catheter between 1999 and 2002.

Results
In five patients (aged 5 months-1.6 yr), the catheter did not thread to the desired level and was abandoned in the operating room (technical success rate, 98.2%). Of the remaining 284 patients, the overall analgesic success rate of all caudal route epidural analgesia procedures was 84.9%. There was no significant difference in adequate pain control (success) in infants (aged 1 day-1 yr) versus older children (aged younger than 1 yr). The most common adverse effects were pruritus (26.1%) and nausea and vomiting (16.9%). Of the patients in our study, 57.7% had urinary catheters in situ; of those who did not have a catheter placed, 20.8% experienced urinary retention. The incidence of respiratory depression was 4.2%, but the administration of naloxone for severe respiratory depression was never necessary. Three percent of catheters were removed because of suspected contamination, but no epidural abscesses or systemic infection were noted.

Conclusions
The results of this study suggest that epidural catheter placement via the caudal approach using the Tsui test is an effective and reasonable alternative to direct lumbar and thoracic epidural analgesia in pediatric patients.
Original Investigations of Nerve Block Pain Management after Anterior Cruciate Ligament Reconstruction
Authors: Williams Brian A. , Kentor Michael L. , Vogt Molly T. , Vogt William B. , Coley Kim C. , Williams John P. , Roberts Mark S. , Chelly Jacques E. , Harner Christopher D. , Fu Freddie H. 
Published: February 2004

Background
Anterior cruciate ligament reconstruction is a complex outpatient surgical procedure often associated with pain. Traditionally, the procedure is performed under general anesthesia and often requires the use of the PACU. Refractory pain and/or nausea/vomiting occasionally leads to an unplanned hospital admission. In this study, the authors examine the associations of nerve block analgesia for these patients and its associated reductions in PACU use, hospital admission, and hospital costs.

Methods
This was an observational, nonrandomized study in which existing data regarding patients' day-of-surgery outcomes were merged with hospital cost data. We reviewed a consecutive sample of 948 men and women who were in good health and underwent anterior cruciate ligament reconstruction in an outpatient surgery unit between July 1995 and June 1999.

Results
The use of nerve block analgesia was associated with reduced PACU admissions to 18% and decreased unplanned hospital admission rates from 17% to 4%. Multivariate linear regression analysis showed that patients bypassing the PACU had an associated hospital cost reduction of 12% (P = 0.0001), whereas patients who needed hospital admission had an associated hospital cost increase of 11% (P = 0.0003).

Conclusions
The use of nerve blocks for acute pain management in patients undergoing anterior cruciate ligament reconstruction is associated with PACU bypass and reliable same-day discharge. Although the cost savings for this one procedure are unlikely to generate sufficient cost savings via staffing reductions, extrapolating these results to a large volume of all types of invasive outpatient orthopedic procedures may have the potential to create significant hospital cost savings.
Histological organization and its relationship to function in the femur of Alligator mississippiensis
Authors: Lee Andrew H. 
Published: February 2004

AbstractHistological analysis of a growth series of alligator femora tests the correlation between strain milieu and microstructure. From mid‐diaphyseal cross‐sections of these femora (n = 7), vascular canal orientation and density as well as collagen fibre organization were recorded. Throughout ontogeny, the proportion of transverse–spiral (TS) collagen in the dorsal cortex is significantly greater than it is in the ventral cortex (P = 0.008). This regional difference in the proportion of TS collagen is correlated with a regional difference in the state of peak principal strain (compressive or tensile). Nevertheless, the predominant orientation of collagen fibres is longitudinal, which is inconsistent with biomechanical hypotheses that involve peak principal or shear strains. Although the density and orientation of vascular canals do not show significant regional differences (P = 0.26 and P = 0.26, respectively), as with collagen orientation, the vascular canal orientation is predominantly longitudinal. The longitudinal organization of both the vascular canals and the collagen fibres is probably a consequence of longitudinal shifting of subperiosteal osteoid during femoral lengthening. When taken together, these data suggest that growth dynamics is the dominant influence on the histological organization of primary bony tissues in alligator femora.
Leaving state sector employment in Russia
Authors: Turunen Jarkko 
Published: February 2004

AbstractI analyse the reallocation of labour and human capital from the state sector to the non‐state sector and non‐employment in Russia. I use a nationally representative household dataset, the Russian Longitudinal Monitoring Survey, to study sectoral mobility in early transition using summary measures of mobility and multivariate discrete choice models. The results show that sectoral mobility varies between different skill groups, and in particular that those with university education, with supervisory responsibility and in white‐collar occupations are less likely to leave state jobs for both non‐state employment and non‐employment. The results suggest that in the early stages of transition in Russia mismatch of skills across state/non‐state employment was significant and that non‐state employment consisted mostly of low skill, ‘bad’ jobs.
Catching up: The role of demand, supply and regulated price effects on the real exchange rates of four accession countries
Authors: MacDonald Ronald , Wójcik Cezary 
Published: February 2004

AbstractThe main aim of this paper is to examine the exchange rate behaviour of a group of four transitional, EU accession countries, with a view to making policy recommendations regarding their full accession to the European Monetary Union. We employ a dynamic OLS panel estimator to investigate the relative importance of demand and supply influences on the exchange rates of these countries. Our analysis shows that both supply‐ and demand‐side effects are important for the accession countries, although their overall effect on inflation differentials and competitiveness seems to be small. An additional focus of the paper is the examination of the role that administrated, or regulated, prices and the productivity of the distribution sector play in the real exchange rate dynamics. Using a unique database we show that administrative prices have been a powerful force behind price and real exchange developments for our group of accession countries. The distribution sector is shown to have an independent effect on the internal price ratio over and above that generated by the Balassa–Samuelson effect.
Ustilago maydis, model system for analysis of the molecular basis of fungal pathogenicity
Authors: Basse Christoph W. , Steinberg Gero 
Published: February 2004

SUMMARY Ustilago maydis, a facultative biotrophic basidiomycete fungus, causes smut disease in maize. A hallmark of this disease is the induction of large plant tumours that are filled with masses of black‐pigmented teliospores. During the last 15 years U. maydis has become an important model system to unravel molecular mechanisms of fungal phytopathogenicity. This review highlights recent insights into molecular mechanisms of complex signalling pathways that are involved in the transition from budding to filamentous growth and operate during the pathogenic growth phase. In addition, we describe recent progress in understanding the structural basis of morphogenesis and polar growth in different stages of U. maydis development. Finally, we present an overview of recently identified genes related to pathogenic development and summarize novel molecular and genomic approaches that are powerful tools to explore the genetic base of pathogenicity. Taxonomy: Ustilago maydis (DC) Corda (synonymous with Ustilago zeae Ung.)‐Kingdom Eukaryota, Phylum Fungi, Order Basidiomycota, Family Ustilaginomycetes, Genus Ustilago. Host range: Infects aerial parts of corn plants (Zea mays) and its progenitor teosinte (Zea mays ssp. parviglumis). Maize smut is distributed throughout the world. Disease symptoms: U. maydis causes chlorotic lesions in infected areas, the formation of anthocyanin pigments, necrosis, hyperplasia and hypertrophy of infected organs. Infection by U. maydis can inhibit development and lead to stunting of infected plants. A few days after infection plant tumours develop in which massive fungal proliferation and the formation of the black‐pigmented, diploid teliospores occurs. Under natural conditions tumours predominantly develop on sexual organs (tassels and ears), stems and nodal shoots. Tumours may vary in size from minute pustules to several centimetres in diameter and contain up to 200 billion spores. Useful web site: http://www‐genome.wi.mit.edu/annotation/fungi/ustilago_maydis/
pthG from Pantoea agglomerans pv. gypsophilae encodes an avirulence effector that determines incompatibility in multiple beet species
Authors: Ezra David , Barash Isaac , Weinthal Dan M. , Gaba Victor , Manulis Shulamit 
Published: February 2004

SUMMARY Pantoea agglomerans pv. gypsophilae (Pag) causes root and crown gall disease on gypsophila, whereas P. agglomerans pv. betae (Pab) induces the disease on beet as well as gypsophila. Both pathovars harbour a pathogenicity plasmid (pPATHPag or pPATHPab) that determines disease development. We have previously isolated and partially characterized a pleiotropic gene from the pPATHPag, designated as pthG, that encodes a virulence factor in gypsophila and an elicitor of a hypersensitive‐like response in beet roots. The present study was undertaken to characterize pthG further as an avr gene. The infiltration of beet leaves with strains expressing PthG (i.e. Pag or Pab containing pthG in trans) caused an hypersensitive reaction (HR) response within 48 h, whereas strains lacking intact pthG (i.e. Pab or Pag mutated in pthG) resulted in gall formation after 5 days. A hypersensitive reaction was elicited by PthG on multiple beet species, whereas a marker exchange mutant of Pag in pthG extended its host range on these beet species. A marker exchange mutant of Pag in hrpJ, encoding a component of the Type III secretion system, prevented HR elicitation. Mutations in each of the hrp regulatory genes (hrpY, hrpS and hrpL) substantially reduced the transcriptional activity of pthG in gypsophila cuttings. PthG could only be detected inside Pag cells during over‐expression of hrpS or hrpL. Particle bombardment of GFP‐PthG fusion caused cell death in beet, but not in non‐host (melon) leaves. Present and previous results have established pthG as a broad‐host‐range avr gene that functions in multiple host plant species and the first functional avr gene in Pantoea spp.
Protection against systemic candidiasis in mice immunized with secreted aspartic proteinase 2
Authors: Vilanova Manuel , Teixeira Luzia , Caramalho Íris , Torrado Egídio , Marques Andreia , Madureira Pedro , Ribeiro Adília , Ferreira Paula , Gama Miguel , Demengeot Jocelyne 
Published: February 2004

SummarySecreted aspartic proteinases (Sap) have been described as virulence factors implicated in the mechanisms of host colonization by the yeast Candida albicans in different types of candidiasis. Intraperitoneal inoculation of C. albicans into BALB/c mice rapidly leads to systemic candidiasis, with significant colonization of the kidneys measurable in the following week. In this study we assessed the potential of vaccination with C. albicans secreted aspartic proteinase 2 (Sap2) in preventing systemic candidiasis in BALB/c mice. Intradermal injection of highly purified native Sap2 protein incorporated in alum adjuvant provided efficient immune protection, as indicated by a 20‐fold decrease in the colonization of kidneys. The protective effect of Sap2 immunization with alum adjuvant was also observed in mice infected with a lethal inoculum of C. albicans. Immunization with the native Sap2 alone, as well as with a denatured recombinant form of the protein, also conferred protection, albeit to a lesser level. In all cases, protection correlated with an increase in serum antibodies to Sap2. Moreover, passive transfer of anti‐Sap2 immunoglobulin G (IgG) significantly decreased the yeast burden in kidneys of C. albicans‐infected mice. This result shows that immune protection against systemic candidiasis in mice immunized with Sap2 is antibody‐mediated. Taken together, these analyses demonstrate that Sap2 can be successfully used as a vaccination target in systemic candidiasis and reveals the potential immunomodulatory role of Sap2 on C. albicans infection.
The Echinococcus granulosus antigen EgA31: localization during development and immunogenic properties
Authors: Saboulard Didier , Lahmar Samia , Petavy Anne‐Françoise , Bosquet Georges 
Published: February 2004

SUMMARYEgA31 is a fibrillar protein from Echinococcus granulosus that behaves as a potent antigen during infestation of dogs. The localization of this antigen during development of the parasite was investigated by immunohistochemistry in optical and electron microscopy. The protein is mostly abundant in the microtriches and subtegumental cells of the adult, whereas it is absent from protoscolex microtriches. Eggs, the periphery of calcareous corpuscles, and the germinal layer were other sites of accumulation. Immunogenicity of different domains of the protein was assessed during experimental infection of dogs. It was shown that the polypeptide encoded by the Pst I–Hind III fragment of the complete cDNA is the most antigenic during the infection. The uses of such polypeptide for infection diagnosis and as a candidate vaccine protein are discussed.
Statistical Analyses and Modelling of the Mixing Process of Euro Coins in Germany and Europe
Authors: Stoyan Dietrich , Stoyan Helga , Döge Gunter 
Published: February 2004

SummaryThis paper reports on the mixing of Euro coins from different countries of origin in Europe, which started on 1 January 2002. There is an interesting conclusion: that the mobility of small and large denominations is different. The long‐term behaviour of the mixing process is studied using a simple deterministic model and data from Germany, France and the Netherlands. The analysis leads to predictions about the future progress of the mixing process.