bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic characterization of the AHAS mutant line K4 with resistance to AHAS-inhibitor herbicides in rapeseed (Brassica napus L.)
Authors: Zhang Yani , Huang Qianxin , Wang Shengnan , Gao Lianliang , Qu Gaoping , Guo Yuan , Hu Zhaoxin , Hu Shengwu 
Published: February 2025

Abstract
It remains a great challenge to control weeds in rapeseed fields in China. Breeding herbicide-resistant rapeseed varieties and using corresponding herbicide formulations has become the most economical and effective way to control weeds in rapeseed field. Characterization of more herbicide-resistant genetic resources will provide opportunities for breeders to develop rapeseed herbicide-resistant varieties with good agronomic performance. Previously, we obtained the tribenuron methyl (TBM)-resistant mutant K4 from ZS9 (Brassica napus L.) through ethyl methyl sulfonate mutagenesis and TBM foliar-spray screening. In this study, the inheritance and molecular characterization of the mutant K4 are carried out. Genetic investigation indicated that the herbicide-resistance of the K4 was controlled by one dominant allele at a single nuclear gene locus. Molecular characterization showed that a single point substitution at position 535 from C to T in BnAHAS3 (BnAHAS3

535T
), which resulted in a mutation at point 179 in BnAHAS3. The K4 showed a certain degree of resistance to TBM, bensulfuron methyl, and monosulfon sodium, which were 50, 30, and 5 times that of ZS9, respectively. AHAS enzyme assay, structural analysis of AHAS proteins, affinity detection between TBM and BnAHAS3 by surface plasmon resonance analysis, and the transgenic experiment in Arabidopsis using BnAHAS3

535T
confirmed that BnAHAS3

535T
endow the K4 with herbicides resistance. In addition, an allele-specific marker was developed to quickly distinguish the heterozygous and homozygous mutated alleles BnAHAS3

535T
. In conclusion, our research identified and characterized one novel mutative AHAS allele in B. napus and enriched genetic resource for developing herbicide-resistant rapeseed cultivars.
Evidential Pluralism and accounts of establishing
Authors: Wilde Michael 
Published: February 2025

Abstract
In Evi
dential Pluralism in the Social Sciences, Yafeng Shan and Jon Williamson do a great job of clarifying, motivating, and defending the commitments of Evidential Pluralism. In this commentary, I will show that one of their clarifications commits Evidential Pluralism to a particular account of establishing. And I will argue that a non-committal account of establishing would better promote the main message of the book.
When forecasting and foresight meet data and innovation: toward a taxonomy of anticipatory methods for migration policy
Authors: Marcucci Sara , Verhulst Stefaan , Cervantes María Esther 
Published: February 2025

Abstract
The various global refugee and migration events of the last few years underscore the need for advancing anticipatory strategies in migration policy. The struggle to manage large inflows (or outflows) highlights the demand for proactive measures based on a sense of the future. Anticipatory methods, ranging from predictive models to foresight techniques, emerge as valuable tools for policymakers. These methods, now bolstered by advancements in technology and leveraging nontraditional data sources, can offer a pathway to develop more precise, responsive, and forward-thinking policies.
This paper seeks to map out the rapidly evolving domain of anticipatory methods in the realm of migration policy, capturing the trend toward integrating quantitative and qualitative methodologies and harnessing novel tools and data. It introduces a new taxonomy designed to organize these methods into three core categories: Experience-based, Exploration-based, and Expertise-based. This classification aims to guide policymakers in selecting the most suitable methods for specific contexts or questions, thereby enhancing migration policies.
Diploicia edulis (Caliciaceae) and Physcia ornamentalis (Physciaceae), two new species associated with invertebrates from the tropical dry forest of Mexico
Authors: Miranda-González Ricardo , Campos-Cerda Felipe , Herrera-Campos María de los Angeles 
Published: February 2025

AbstractTwo new species in the genera Diploicia and Physcia are described from the tropical dry forest of Mexico. Both species are supported by morphological, chemical and molecular evidence. Diploicia edulis, a species heavily consumed by invertebrates, is characterized by lecanorine apothecia, a dull brown epihymenium not diffused by a green pigment (K−), a subhymenium conspicuously inspersed with oil droplets, and the diploicin chemosyndrome. We provide the first molecular evidence to support the inclusion of species with lecanorine apothecia in the genus Diploicia. Physcia ornamentalis, previously reported under the name Physcia undulata s. lat. as one of the main construction materials for the bags of a moth caterpillar species (Psychidae), is characterized by a frosted-pruinose thallus, soralia originating in the lobe sinuses, and by lacking soralia in the thalline margin of the apothecia.
Popular music studies behind the Iron Curtain: the constitution of popular music research in East-Central Europe before 1989
Authors: Wicke Peter 
Published: February 2025

Abstract
The study of popular music in the Soviet-controlled East-Central European region has yet to receive the attention it merits. The objective of this study was to fill this gap by investigating the genealogy of popular music research in East Central Europe prior to 1989. It provides a comprehensive account of the evolution of popular music research in socialist East-Central Europe, with a particular focus on Czechoslovakia, Hungary and East Germany. It delineates the challenges encountered by researchers in reconciling their work with the complex cultural and political conditions of the era. Furthermore, it considers the influence of Marxist ideology on musicology and sociology, and how this may have shaped research. It offers new perspectives on the history of popular music studies in this domain, which has been lightly explored until now. It seeks to shed light on the intricate relationship between music, politics and ideology behind the Iron Curtain.
Student Choice in Assessment: Is There a Subject-Specific Case for Using Assessment Optionality in Political Science Education?
Authors: Moulton Jeremy F. G. 
Published: February 2025

ABSTRACT
Giving students choice in how they are assessed, known as assessment optionality, is an innovation in assessment that has gained attention in recent years for its potential for improving inclusivity and engagement in teaching and learning. However, although assessment innovations can be beneficial, the process of changing long-established norms can be difficult. Political science is a field in which the Scholarship of Teaching and Learning literature has long noted that there is limited pedagogical innovation. This resistance to innovation creates a challenge for those working in the field—stronger and specific arguments must be developed to justify changes to curricula. This article presents the results of participatory research with political science students to explore whether a subject-specific case can be made for the use of assessment optionality in the discipline. The study explores political science students’ understandings of costs and benefits of assessment optionality and shares co-created recommendations for the potential application of the practice.
A Paradox of Pleasure: Black Joy during “the Nadir,” 1875‒1905
Authors: Purkiss Ava 
Published: February 2025

AbstractThis article revisits Rayford Logan’s thesis in The Negro in American Life and Thought: The Nadir, 1877‒1901 to chart how African Americans experienced joy during a racial low point—“the Nadir” of race relations. Using Logan’s claims as a conceptual framework, the article examines W. E. B. Du Bois’s position on amusement and everyday Black people’s joyful acts during the post-Reconstruction period to understand “a paradox of pleasure”—feeling jovial during dark times. With the Nadir as a case study, this essay argues that historians may develop Black joy as a historical analytic by asking research questions about Black affect, employing the tools of historical imagination, and concentrating on the small delights of daily life. This essay seeks to inspire curiosity about how exploring Black life from the angle of elation, not sorrow, can produce complex histories of Black subjectivity and feeling. It proposes Black joy as an inchoate analytic in hopes of it becoming a formal mode of historical inquiry.
“The Old Order Changeth”: A Favorite Servant Contest and the Debate about Domestic Work in Washington, D.C.
Authors: Alms Hannah 
Published: February 2025

AbstractThe Progressive Era was characterized by debates about the future of the United States and the role of individuals, households, and organizations in shaping that future. These debates included those about domestic work, sometimes specifically referred to as the “servant question” or the “servant problem.” This discourse considered not only paid household labor, but also the nature of race, gender, and American life after slavery. This article reviews the servant question in Washington, D.C., and reveals how commentators engaged with modernity and nostalgia to understand the contradiction between their sense of white and African American women’s failures and their belief that both groups of women belonged in white households. The servant question is key cultural context for a 1917 “favorite servant contest.” The second half of the article examines the clubwomen who organized the contest and the experiences of an elderly, formerly enslaved woman named Theresa Harper. The organizers responded to the D.C. servant question with an effort to carry racial hierarchies into the twentieth century, a vision of the future of household labor very different from that of Black domestic workers.
On Patrons and Shoppers: Representations of Consumer Culture in the Black Press from 1890 to 1920
Authors: Regev Ronny 
Published: February 2025

AbstractThis article traces the early origins of Black consumer culture as it was portrayed in the Black press from the late 1800s to the early 1920s. It argues that Black newspapers were important agents in shaping how African Americans conceived of and interacted with the evolving commercial sphere around the turn of the century. Papers such as the Pittsburgh Courier, the Broad Ax, the Tulsa Star, and many others celebrated participation in the consumer arena as a respectable and desired practice. They also distinguished between shopping, as a social feminine pursuit, and patronizing Black-owned businesses, which was perceived as a gender-neutral, or even manly, racial duty. Espousing African American elite ideologies such as racial uplift and self-help, Black editors presented any purchasing of goods as an upright activity, which adorned its performer with affluence, respect, and power. Such portrayal encouraged the participation of African Americans in the consumer sphere and implied that it was an arena of similarity rather than difference.
Comparison of recognition of symptom burden in MPN between patient- and physician-reported assessment – an intraindividual analysis by the German Study Group for MPN (GSG-MPN)
Authors: Manz Kirsi , Heidel Florian H. , Koschmieder Steffen , Schlag Rudolf , Lipke Jörg , Stegelmann Frank , Griesshammer Martin , Klausmann Martine , Crodel Carl , Hochhaus Andreas , Schulz Holger , Göthert Joachim R. , Al-Ali Haifa , Becker Heiko , Reiter Andreas , Beutel Gernot , Kricheldorf Kim , Brümmendorf Tim H. , Hoffmann Wolfgang , Döhner Konstanze , Isfort Susanne ,   On behalf of the German Study Group for Myeloproliferative Neoplasms (GSG-MPN)
Published: February 2025

Abstract
Myeloproliferative neoplasms (MPN) are associated with a variety of symptoms that severely impact patients’ quality of life and ability to perform daily activities. Recent studies showed differences in the perception of physician- versus patient-reported symptom burden. However, studies directly comparing patient- and physician-reported ratings are lacking. Here, a retrospective analysis on symptom burden of 3979 MPN patients of the Bioregistry of the German Study Group for MPN was conducted to intra-individually compare physician and patient reports collected at the same time. Cohen’s kappa was calculated to assess the degree of agreement between patient and physician reports. Factors influencing baseline symptom severity were identified using linear regression and adjusted Cox models were calculated to investigate the effect of symptom burden on survival. MPN patients had a high symptom burden, which neither decreased over time nor upon cytoreductive therapy. All symptoms were more frequently reported by patients compared to physicians. Agreement remained low and only slightly improved when considering a higher threshold for patient symptom severity. Patients with severe symptom burden had inferior survival compared to patients with less severe symptoms. Assessment of symptom burden in MPN is therefore insufficient and patient-reported outcome tools need to be implemented into clinical routine.
NFIX suppresses breast cancer cell proliferation by delaying mitosis through downregulation of CDK1 expression
Authors: Ma Hai-Yan , Sun Rui , Tian Tian , Zhou Xue-Jie , Chen Zhao-Hui , Cao Xu-Chen , Yu Yue , Wang Xin 
Published: February 2025

Abstract
One of the fundamental biological characteristics of malignant tumors is their uncontrolled growth and multiplication, which is a major reason why breast cancer remains incurable. The significance of NFIX in the development of various cancers has been demonstrated by an increasing number of studies in recent years. However, the role of NFIX in breast cancer has received less attention. This study investigates its expression in breast cancer and its function in inhibiting cell cycle progression. NFIX is downregulated in breast cancer compared to normal breast tissue, which impacts prognosis. In vitro and in vivo Experiments have shown that the overexpression of NIFX leads to a delay in the G2/M phase, which inhibits breast cancer cell proliferation. It thus plays a role as a tumor suppressor in breast cancer development. In terms of mechanism, upregulating NFIX causes CDK1 to be more susceptible to ubiquitination-mediated degradation. NFIX also competitively represses CDK1 transcription via YBX1. Moreover, NFIX expression in breast cancer is associated with methylation of its promoter region. Our study demonstrated that NFIX plays a critical role in CDK1-regulated cell cycle transitions and determined that NFIX inhibits cell proliferation in breast cancer.
A column-like organization for ocular dominance in mouse visual cortex
Authors: Goltstein Pieter M. , Laubender David , Bonhoeffer Tobias , Hübener Mark 
Published: February 2025

Abstract
The columnar organization of response properties is a fundamental feature of the mammalian visual cortex. However, columns have not been observed universally across all mammalian species. Here, we report the discovery of clusters of ipsilateral eye preferring neurons in layer 4 of the mouse primary visual cortex. These clusters extend into layer 2/3 and upper layer 5, forming a column-like pattern for ocular dominance. Our observation of such structures in this minute cortical area sets a new boundary condition for models explaining the emergence of functional organizations in the neocortex.
Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activities
Authors: Zhou Linjiao , Ortega-Rodriguez Uriel , Flores Matthew J. , Matsumoto Yasuyuki , Bettinger John Q. , Wu Wells W. , Zhang Yaqin , Kim Su-Ryun , Biel Thomas G. , Pritts Jordan D. , Shen Rong-Fong , Rao V. Ashutosh , Ju Tongzhong 
Published: February 2025

Abstract
Mucin-type O-glycans on glycoproteins are pivotal for biology and impact the quality of biotherapeutics. Furthermore, glycans on host cells serve as ligands for lectins/adhesins on bacteria for bacterium-host interactions in the colonization or attachment/invasion of bacteria. Defining the structure-function relationship of O-glycans is hindered by a lack of enzyme(s) to release sialylated O-glycans from glycoproteins. Here we show identification of endo-α-N-acetylgalactosaminidases (O-glycanases, GH101) with broad substrate specificities, termed Peptide:O-Glycosidase (POGase). In 5 POGase orthologs identified, we characterize one that releases sialylated O-glycans from glycopeptides, glycoproteins and biotherapeutics. Three peptide motifs differentiate the POGase existing in phylum Actinomycetota from known O-glycanases in other bacteria. While the GH101 domain classifies POGases, other domains confer the efficient enzyme activity and binding to major glycans decorating epithelial cells. The dual functional POGases encompassing broader O-glycanase and adhesin activities will facilitate the study of O-glycomics, quality assessment of biotherapeutics, and development of microbiology and medicine.
KAT6B overexpression rescues embryonic lethality in homozygous null KAT6A mice restoring vitality and normal lifespan
Authors: Bergamasco Maria. I. , Yang Yuqing , Garnham Alexandra L. , Sheikh Bilal N. , Smyth Gordon K. , Voss Anne. K. , Thomas Tim 
Published: February 2025

Abstract
Closely related genes typically display common essential functions but also functional diversification, ensuring retention of both genes throughout evolution. The histone lysine acetyltransferases KAT6A (MOZ) and KAT6B (QKF/MORF), sharing identical protein domain structure, are mutually exclusive catalytic subunits of a multiprotein complex. Mutations in either KAT6A or KAT6B result in congenital intellectual disability disorders in human patients. In mice, loss of function of either gene results in distinct, severe phenotypic consequences. Here we show that, surprisingly, 4-fold overexpression of Kat6b rescues all previously described developmental defects in Kat6a mutant mice, including rescuing the absence of hematopoietic stem cells. Kat6b restores acetylation at histone H3 lysines 9 and 23 and reverses critical gene expression anomalies in Kat6a mutant mice. Our data suggest that the target gene specificity of KAT6A can be substituted by the related paralogue KAT6B, despite differences in amino acid sequence, if KAT6B is expressed at sufficiently high levels.
Structural basis for lipid-mediated activation of G protein-coupled receptor GPR55
Authors: Claff Tobias , Ebenhoch Rebecca , Kley Jörg T. , Magarkar Aniket , Nar Herbert , Weichert Dietmar 
Published: February 2025

Abstract
GPR55 is an orphan G protein-coupled receptor (GPCR) and represents a promising drug target for cancer, inflammation, and metabolic diseases. The endogenous activation of lipid GPCRs can be solely mediated by membrane components and different lipids have been proposed as endogenous activators of GPR55, such as cannabinoids and lysophosphatidylinositols. Here, we determine high-resolution cryo-electron microscopy structures of the activated GPR55 in complex with heterotrimeric G13 and two structurally diverse ligands: the putative endogenous agonist 1-palmitoyl-2-lysophosphatidylinositol (LPI) and the synthetic agonist ML184. These results reveal insights into ligand recognition at GPR55, G protein coupling and receptor activation. Notably, an orthosteric binding site opening towards the membrane is observed in both structures, enabling direct interaction of the agonists with membrane lipids. The structural observations are supported by mutagenesis and functional experiments employing G protein dissociation assays. These findings will be of importance for the structure-based development of drugs targeting GPR55.
Discrimination of inherent characteristics of susceptible and resistant strains of Anopheles gambiae by explainable artificial intelligence analysis of flight trajectories
Authors: Qureshi Yasser M. , Voloshin Vitaly , Gleave Katherine , Ranson Hilary , McCall Philip J. , Covington James A. , Towers Catherine E. , Towers David P. 
Published: February 2025

Abstract
Understanding mosquito behaviours is vital for the development of insecticide-treated nets (ITNs), which have been successfully deployed in sub-Saharan Africa to reduce disease transmission, particularly malaria. However, rising insecticide resistance (IR) among mosquito populations, owing to genetic and behavioural changes, poses a significant challenge. We present a machine learning pipeline that successfully distinguishes between innate IR and insecticide-susceptible (IS) mosquito flight behaviours independent of insecticidal exposure by analysing trajectory data. Data-driven methods are introduced to accommodate common tracking system shortcomings that occur due to mosquito positions being occluded by the bednet or other objects. Trajectories, obtained from room-scale tracking of two IR and two IS strains around a human-baited, untreated bednet, were analysed using features such as velocity, acceleration, and geometric descriptors. Using these features, an XGBoost model achieved a balanced accuracy of 0.743 and a ROC AUC of 0.813 in classifying IR from IS mosquitoes. SHAP analysis helped decipher that IR mosquitoes tend to fly slower with more directed flight paths and lower variability than IS—traits that are likely a fitness advantage by enhancing their ability to respond more quickly to bloodmeal cues. This approach provides valuable insights based on flight behaviour that can reveal the action of interventions and insecticides on mosquito physiology.
Acute effects of high-intensity interval training and moderate-intensity continuous training on executive functions in healthy older adults
Authors: Ahmadi Shirko , Bélanger Mathieu , O’Brien Myles W. , Registe Pierre Philippe Wilson , Dupuy Olivier , Mekari Said 
Published: February 2025

Abstract
Numerous studies have demonstrated that executive functions benefit from high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT). However, the immediate effects of HIIT and MICT on these functions in older adults have not been compared. This study aimed to examine the acute impact of HIIT and MICT on executive function components in this demographic. Twenty-five healthy community-dwelling older adults (15 females; average age 67.1 ± 4.5 years) participated. The study involved three sessions: an initial session with cognitive assessments (Stroop Task: Naming, Inhibition, and Switching) and a maximal continuous graded exercise test, followed by two sessions involving HIIT (15s at 100% peak power output, 15s rest, 2 × 20 min) or MICT (34 min at 60% peak power output) training protocols in random order. Cognitive tests were administered immediately after and 45 min post-training. The results showed a significant difference in Switching reaction times between MICT and HIIT, with HIIT showing a greater reduction in Switching times after 45 min (p = 0.019). In conclusion, our study indicates that HIIT’s beneficial effects on executive functions demonstrated a larger effect size than those of MICT. This suggests that brief, high-intensity exercise could be more effective in enhancing executive functions among older adults.