bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of manual restorative treatment (MRT) with amalgam in high‐caries Filipino children: results after 2 years
Authors: Monse‐Schneider B. , Heinrich‐Weltzien R. , Schug D. , Sheiham A. , Borutta A. 
Published: December 2004

Abstract –Background: The atraumatic restorative treatment (ART), using only hand instruments and glass‐ionomer cement as adhesive material is recommended for restorative dental treatment in disadvantaged communities lacking electricity and sophisticated dental equipment. Research is required on more durable restorative materials appropriate for populations with high‐caries experience.Objectives: The aim of the study was to evaluate, under field conditions, the applicability and effectiveness of an encapsulated amalgam as restorative material in ART prepared cavities in permanent teeth of children with high‐caries rates. As the definition of ART restricts the manual treatment to adhesive materials the approach used is called the manual restorative treatment (MRT).Methods: Two dentists and two trained healthcare workers, using hand instruments and an encapsulated amalgam that was mixed with a manually driven triturator, placed a total of 934 restorations in the permanent dentition in 466 children. Due to irregular school attendance of Filipino children only 611 restorations could be evaluated by one independent dentist. The average age at reassessment of restorations was 27 ± 4.5 months.Results: 93.3% of the restorations were acceptable. The failure rate of occlusal surface restorations was 5.6% compared with 13.6% of buccal surface fillings. The survival rate of large occlusal restorations was 95.1% compared with 93.7% for small restorations. The survival of MRT restorations was not influenced by the experience and professional level of the operator.Conclusions: Amalgam is a suitable MRT material, especially for extensive occlusal lesions in high‐caries populations. Studies of longer duration are needed to confirm this finding.
Dental caries in postwar Bosnia and Herzegovina
Authors: Ivanković Ante , Lukić Ivan Krešimir , Ivanković Zorana , Radić Ankica , Vukić Iljo , Šimić Ankica 
Published: December 2004

Abstract –Objectives: To present the prevalence of dental caries in postwar Bosnia and Herzegovina (BH).Methods: A survey focused on dmft and DMFT indices was carried out in four cantons of the Federation of BH in 1997. The dental health of 6‐ and 12‐year‐olds was assessed in random samples (n = 238 and 318, respectively). DMFT index in adult population was assessed in clients coming to dental offices in the same area (35–44‐year‐olds; n = 401). All the subjects were clinically investigated.Results: During the 1991–95 war, all four cantons were affected by the migration of population. The population per dentist ratio considerably increased in three cantons. In 6‐year‐olds, the average dmft (±SD) was 4.9 ± 4.0, and 86% of the children were affected with dental caries. The average DMFT of 12‐year‐olds was 6.2 ± 3.9. On the average, 94% of the 12‐year‐olds were affected with dental caries. Mean DMFT of adult clients was 15.1 ± 7.0. Almost every adult (98%) was affected with dental caries.Conclusions: The dental status in postwar BH is rather poor and the mean DMFT in all investigated age groups may be considered high. The detrimental effect of 1991–95 war on social conditions and the health care system should be considered in explaining such poor dental health. Despite the limitations, the presented data may be used to assess the treatment needs, assure the visibility for dental issues in BH, and help the west European countries to plan dental services for refugees from BH.
Antibody to S100a6 protein is a sensitive immunohistochemical marker for neurothekeoma
Authors: Fullen Douglas R. , Lowe Lori , Su Lyndon D. 
Published: December 2004

Background: Neurothekeoma is a benign tumor of putative peripheral nerve sheath origin. It occurs in a myxoid (classic) variant, cellular variant, and intermediate (mixed) variant. Cellular neurothekeoma (CNT) usually involves the head and neck or extremities of young patients. Histologically, CNT can be confused with melanocytic and fibrohistiocytic lesions. An immunohistochemical antibody panel is often necessary to confirm the histological impression and exclude melanocytic and/or fibrohistiocytic lesions.Methods: Formalin‐fixed, paraffin‐embedded archival tissues were evaluated by immunohistochemistry using antibodies specific for S100A6 and PGP9.5 in 11 cases of neurothekeoma (seven cellular, four myxoid). A variety of other antibodies were evaluated by immunohistochemistry at the time of initial diagnosis.Results: All 11 neurothekeoma cases were positive for S100A6 protein (four cases, weak/1+ ; seven cases, strong/2+), corresponding to 100% sensitivity. In contrast, eight of 11 neurothekeoma cases (73% sensitivity) were positive for PGP9.5. All seven CNT cases were negative for S100B, as expected.Conclusions: Anti‐S100A6 is a highly sensitive antibody for neurothekeomas, including CNT, and, in our experience, is superior in sensitivity to PGP9.5. However, like other antibodies used in evaluating neurothekeomas, S100A6 lacks specificity, as has been demonstrated in previous studies. Nevertheless, S100A6 can be useful in an immunohistochemical antibody panel to evaluate lesions where the differential diagnosis includes CNT.
Effects of biopsy‐induced wound healing on residual basal cell and squamous cell carcinomas: rate of tumor regression in excisional specimens
Authors: Swetter Susan M. , Boldrick Jennifer C. , Pierre Peterson , Wong Patricia , Egbert Barbara M. 
Published: December 2004

Background: Wound healing following a partial biopsy of basal cell (BCC) and squamous cell carcinomas (SCC) may induce tumor regression.Methods: Nonmelanoma skin cancer (NMSC) biopsy and re‐excision specimens from 1994 to 2001 were reviewed for histologic evidence of scar vs. presence of residual tumor in excision specimens. Regressed and non‐regressed tumors were analyzed to assess the influence of anatomic location, biopsy technique (punch vs. shave), histologic subtype of BCC or SCC, time interval between biopsy and excision, and patient age.Results: Nine hundred and ten excisions were performed for transected BCC or SCC, 217 (24%) of which showed scar with no residual tumor. Logistic regression analysis revealed significant differences in the regressed vs. non‐regressed subsets. SCCs were more likely to regress than BCCs (40% vs. 20%, respectively, p < 0.00001). Independent of the NMSC type, tumors regressed more often following shave rather than punch biopsy (34% vs. 15%, respectively, p < 0.00001), as did tumors on the trunk and extremities compared with head and neck cases (31% vs. 21%, respectively, p < 0.01).Conclusions: In our series, 24% of NMSCs transected on the initial biopsy showed no residual tumor in the excision specimens, implying that some event in the interval between biopsy and excision may lead to the eradication of residual tumor. The exact mechanism is unclear, but wound healing likely plays an important role.
Fibroblastic/myofibroblastic sarcoma of the skin: a report of five cases
Authors: Diaz‐Cascajo Carlos , Borghi Susanna , Weyers Wolfgang , Metze Dieter 
Published: December 2004

Background: A number of malignant soft tissue tumors, particularly those of fibroblastic and fibrohistiocytic derivation, have been found to display myofibroblastic differentiation focally. The term myofibroblastic sarcoma, a controversial presumably distinctive entity, defines a malignant soft tissue tumor in which myofibroblasts are quantitatively the predominant cell type.Methods: Five cases of cutaneous spindle‐cell sarcomas showing fibroblastic‐myofibroblastic differentiation with predominance of fibroblasts were retrieved from the files of three large centers of dermatopathology. Tumors were analyzed histopathologically, immunophenotypically, and, in two cases, ultrastructurally. Results were compared with those previously reported in fibrosarcoma, malignant fibrous histiocytoma, and myofibroblastic sarcoma.Results: Immunophenotypic and ultrastructural profiles of the cases analyzed in this series were closer to fibrosarcoma and to malignant fibrous histiocytoma than to myofibroblastic sarcoma by virtue of quantitative predominance of fibroblasts over myofibroblasts. On the other hand, histopathologic findings were in keeping with those reported in myofibroblastic sarcoma.Conclusions: Our series highlights the intrinsic problems in attaching certain cutaneous sarcomas with fibroblastic‐myofibroblastic differentiation to one of the recognized entities and gives support to the hypothesis that fibrosarcoma, malignant fibrous histiocytoma, and myofibroblastic sarcoma are related histogenetically.
Epithelioid angiosarcoma of the breast involving the skin: a highly aggressive neoplasm readily mistaken for mammary carcinoma
Authors: Fariña M. C. , Casado V. , Renedo G. , Estévez L. , Martín L. , Requena L. 
Published: December 2004

Background: Angiosarcomas are malignant neoplasms of endothelial cells. Angiosarcoma of the breast is a rare neoplasm that behaves in a highly malignant fashion. It must be differentiated from benign vascular proliferations and from mammary carcinoma.Methods: We report on a 49‐year‐old‐woman who presented with a large mass involving the left breast.Results: The lesion had an erythematoviolaceous hue and livedoid pattern at the periphery. Histopathologic study showed an epithelioid malignant neoplasm, and immunohistochemical studies demonstrated that neoplastic cells expressed immunoreactivity for endothelial cell markers.Conclusions: A diagnosis of epithelioid angiosarcoma of the breast was established. The patient was treated with radical mastectomy, but she refused any other additional therapy.
Hepatitis C and G viruses in B‐cell lymphomas of the skin
Authors: Michaelis Sonja , Kazakov Dmitry V. , Schmid Mirka , Dummer Reinhard , Burg Günter , Kempf Werner 
Published: December 2004

Background:  The etiology of B‐cell lymphoproliferative disorders (LPDs) of the skin has still to be elucidated. So far, Borrelia sp. has been identified as the causative agent of some cases of B‐cell LPDs of the skin. Apart from bacterial pathogens, recent studies suggested that also flaviviruses, in particular hepatitis C (HCV) and G (HGV) viruses, may be involved in the pathogenesis of B‐cell non‐Hodgkin's lymphomas (NHLs). Most studies were performed in patients with known HCV infection, but the overall frequency of HCV‐ and HGV‐RNA in tumoral tissue of primary cutaneous B‐cell lymphomas (CBCLs) is unknown.Methods:  We examined 23 tumor biopsies of various forms of CBCLs by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and sequencing for the presence of HCV and HGV.Results:  HCV‐RNA sequences were detected in seven of 23 (30%) of the tumor biopsies. In contrast, RNA sequences of HGV were not detected in any of the tumors.Conclusions:  Interestingly, the presence of HCV in our series of primary CBCLs was not restricted to a distinct clinicopathologic subform. HCV which can infect B cells, may play a role in pathogenesis of one‐third of CBCLs, whereas HGV is not involved in CBCLs. Further molecular studies and therapeutic trials are needed to clarify the putative pathogenetic role of HCV in CBCLs.
Use of foraging habitats by bats in a Mediterranean area determined by acoustic surveys: conservation implications
Authors: Russo Danilo , Jones Gareth 
Published: December 2004

We determined habitat use by foraging bats by broad‐band acoustic surveys in 10 habitat types from a Mediterranean area (southern Italy). We applied discriminant functions to identify time‐expanded echolocation calls from free‐flying bats.Moon phase and cloud cover had no effect on bat activity. Only Hypsugo savii was influenced by temperature, and activity of Myotis daubentonii and Myotis capaccinii was reduced at higher wind speeds. Both total numbers of bat passes and feeding buzzes were highest over rivers and lakes. Pipistrellus kuhlii and H. savii were most frequently recorded. Pipistrellus kuhlii, Pipistrellus pipistrellus and Tadarida teniotis proved generalists in using foraging habitats.Water sites and conifer plantations were respectively the most and the least used habitats by H. savii. Rivers were especially important to Myotis bats, Miniopterus schreibersii and Pipistrellus pygmaeus. Unlike P. kuhlii, P. pipistrellus was frequent in beech woodlands; P. pygmaeus made a considerable use of chestnut woodlands and Myotis spp. were moderately active in both these woodland types.A large number of endangered or vulnerable species featured in riparian habitats, broadleaved woodlands and olive groves. Riparian and woodland habitats constitute an important target for conservation. Typical land use forms such as woodlands used for chestnut production and traditionally managed olive groves should be encouraged in conservation plans. The negative impact of urbanisation on bats might be counteracted by fostering trees, gardens and small cultivated patches. Farmland practices should encourage landscape complexity and limit the use of pesticides.
Influenza vaccine in chronic lymphoproliferative disorders and multiple myeloma
Authors: Rapezzi Davide , Sticchi Laura , Racchi Omar , Mangerini Rosa , Ferraris Anna Maria , Gaetani Gian Franco 
Published: December 2004

Abstract:Objective: Vaccination against influenza in patients with chronic lymphoproliferative disorders (CLPD) and multiple myeloma (MM) is still a matter of clinical uncertainty. The aim of this study was to determine the safety, immunogenicity and clinical response to a commercially available vaccine against influenza in a group of such patients.Methods: Thirty‐four patients with CLPD and MM and 34 immunologically normal subjects were vaccinated with the same vaccine against influenza. Patients were observed during the epidemic season from October 1999 to April 2000, and monitored for side‐effects of the vaccine, seroprotection and seroconversion after vaccination. The prevaccination level of immunoglobulins was also determined. Occurrence of influenza episodes was demonstrated with the positive isolation of a viral strain from a pharyngeal swab.Results: No patient had untoward reactions to the vaccine used. Seroconversion and seroprotection were up to the standard established by the European Agency for the Evaluation of Medicinal Products. Only one patient developed influenza during follow‐up.Conclusions: Influenza vaccine is effective and well tolerated in patients with CLPD and MM. No contraindications exist for its use, and it should become a routine practice, in order to prevent serious complications during the influenza epidemic season.
Human platelets express hemochromatosis protein (HFE) and transferrin receptor 2
Authors: Hannuksela Jokke , Parkkila Seppo , Waheed Abdul , Britton Robert S. , Fleming Robert E. , Bacon Bruce R. , Sly William S. 
Published: December 2004

Abstract: Objectives: While body iron status may influence platelets, little information is available about platelet expression of proteins regulating iron homeostasis. HFE, the protein defective in hereditary hemochromatosis, and transferrin receptor 2 (TfR2) are two novel protein candidates that could be involved in mechanisms of iron transport across the platelet plasma membrane.Methods: The expression and localization of HFE, TfR1 and TfR2 proteins in human platelets were examined using Western blotting and immunocytochemistry.Results: Human platelets expressed HFE and TfR2, whereas no signal for TfR1 was found. The positive reactions for HFE and TfR2 were mainly confined to the platelet plasma membrane.Conclusions: Expression of HFE and TfR2 proteins in human platelets may indicate that the mutations in the corresponding genes could influence platelet count, size and/or activation. The presence of TfR2 and absence of TfR1 suggests that HFE may serve a different function in platelets compared with the other HFE‐positive cell types, e.g. enterocytes, macrophages and syncytiotrophoblasts.
Differentiation of follicular dendritic sarcoma cells into functional myeloid‐dendritic cell‐like elements
Authors: Porta Matteo Della , Rigolin Gian Matteo , Bugli Anna Maria , Bardi Antonella , Bragotti Letizia Zenone , Bigoni Renato , Cuneo Antonio , Castoldi Gianluigi 
Published: December 2004

Abstract: In a rare case of follicluar dendritic cell sarcoma, malignant cells were isolated and cultured in the presence of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), interleukin 4 (IL‐4) and tumor necrosis factor alpha (TNF‐α). After 14 d, 15% of neoplastic cells differentiated into myeloid‐dendritic cell‐like elements as demonstrated by immunological and functional characteristics.These cells showed the same cytogenetic abnormality of the malignant clone (fluorescence in situ hybridisation analysis performed on CD1a+ cells) and were able to induce allogenic T‐cell proliferation in the mixed leucocyte reaction.These data may indicate that antigen presenting capacity could be a functional state inducible in cellular elements which are believed not to be of hemopoietic origin.Further studies are warranted to confirm these findings and to clarify the possibility to use these cells to generate specific anti‐tumoral immune responses.
Emergence of Philadelphia positive chronic myeloid leukaemia during treatment with hydroxyurea for Philadelphia negative essential thrombocythaemia
Authors: Wahlin Anders , Golovleva Irina 
Published: December 2004

Abstract:A 58‐yr‐old male patient with essential thrombocythaemia (ET) developed chronic myeloid leukaemia (CML) after continuous uneventful treatment with hydroxyurea for 18 yr. G‐banding analyses of bone marrow cells had repeatedly demonstrated normal male karyotype before this event, but t(9;22)(q34;q11) was demonstrated after the event. An unrelated allogeneic stem cell transplantation was performed. Blood and bone marrow morphology, and the karyotype were normalised and the patient is in good health 1 yr post‐transplant. There are no previous reports in the literature about the emergence of CML during treatment with hydroxyurea.
Salvage treatment with etoposide (VP‐16), ifosfamide and cytarabine (Ara‐C) for patients with recurrent primary central nervous system lymphoma
Authors: Arellano‐Rodrigo Eduardo , López‐Guillermo Armando , Bessell Eric M. , Nomdedeu Benet , Montserrat Emili , Graus Francesc 
Published: December 2004

Abstract:  Background: Survival of patients with primary central nervous system lymphoma (PCNSL) has improved with methotrexate‐based combination regimens and radiotherapy (RT). However, the prognosis of patients who fail or relapse after initial response is poor. Very little data is available on salvage treatment at recurrence.Patients and methods: Sixteen immunocompetent patients (13 males/three females, median age 54 yr) with refractory (one patient) or recurrent (15 patients) PCNSL, homogeneously treated at diagnosis with the cyclophorphamide, doxorubicin, Vimcritime, dexamethasome/carmuntime, Uimcritime, cytarabine and methotrexate (CHOD/BVAM) and RT regimen, received etoposide (VP‐16), ifosfamide and cytarabine (Ara‐C) (VIA) chemotherapy as a salvage treatment. VIA included etoposide 100 mg/m2/d days 1–3, ifosfamide 1000 mg/m2/d days 1–5, and cytarabine 2000 mg/m2/12 h day 1. The therapy was repeated every 28 d for a total of planned six cycles.Results: Median time between first complete response (CR) and relapse was 19 months (range: 6–46 months). Thirteen patients (81%) had a performance status ≤2, six had multifocal PCNSL and six (of eight tested) positive cerebrospinal fluid cytology. The median number of courses per patient was four (range: 1–6). Five patients completed the whole VIA therapy. Six patients (37%) achieved CR. After a median follow‐up of 15 months for surviving patients, two have relapsed, with a median failure‐free survival of 5 months. Twelve patients have died from progression of PCNSL, with a 12‐month overall survival of 41% [95% confidence interval (CI): 16–66]. The major toxicity was World Health Organization grade 2–4 neutropenia (69% of patients) and thrombocytopenia (50%). Five patients had grade 3–4 infectious complications. Finally, one patient developed a severe but reversible ifosfamide encephalopathy.Conclusion: The data presented show that the chemotherapy VIA is an effective salvage regimen for patients with recurrent PCNSL.
Enzyme replacement therapy reduces Gaucher cell burden but may accelerate osteopenia in patients with type I disease – a histological study
Authors: Rudzki Zbigniew , Okoń Krzysztof , Machaczka Maciej , Rucińska Małgorzata , Papla Bolesław , Skotnicki Aleksander B. 
Published: December 2004

Abstract:Enzyme replacement treatment (ERT) is effective in controlling the clinical and biochemical manifestation of type I Gaucher disease. Little is known on the evolution of bone marrow histology caused by ERT. We compared the pretreatment trephine bone marrow biopsies in five patients (F32, F41, F50, M55, and M46) with the control biopsies performed after 26–32 months of imiglucerase treatment. The planimetric estimates revealed significant decrease in Gaucher cell burden in all the patients. The post‐ERT values ranged from 24% to 65% of the initial total volumes occupied by the Gaucher cells. This resulted mainly from disappearance of Gaucher cells, and to a lesser extent from their shrinkage. Normal hemopoiesis was markedly increased in four of five patients, fat tissue in all the five patients. Surprisingly, the estimated volumes of trabecular bone in the control biopsies were significantly smaller than in the pre‐ERT trephines (0.61%, 0.64%, 0.64%, 0.97%, and 0.22% of the initial volumes, P = 0.043). The bone loss correlated rather with the degree of reconstitution of normal hemopoiesis than with the decrease in the Gaucher cell burden. The histological response did not correlate strictly with initial clinical parameters and the genotype, with different reactions to ERT in two sibs (cases 3 and 4). Particularly, ERT alleviated bone manifestations in all four patients with previous bone pains or fractures. ERT may accelerate progress of osteopenia in men and premenopausal women. The clinical significance of this phenomenon remains to be established. Its mechanism may be linked to increased osteolytic activity exerted directly or indirectly by regenerating hemopoietic cells.
Altered cytokeratin expression in actinic cheilitis
Authors: Dos Santos Jean N. , De Sousa Suzana O. M. , Nunes Fábio D. , Sotto Miriam N. , De Araújo Vera C. 
Published: December 2004

Background:  Actinic cheilitis (AC) is a widely recognized precancerous lesion of the lip. Varying degrees of epithelial dysplasia may be present. However, no studies have correlated epithelial changes with cytokeratin expression that might reflect the disordered maturation that is probably occurring.Methods:  Thirty‐four cases diagnosed as AC were classified according to dysplasia degree, and submitted to immunohistochemical staining for the detection of cytokeratins (CKs) 7, 8, 13, 14, 16 and 19. Normal mucosa adjacent to the lesions was also evaluated.Results:  The results obtained showed that CK10 immunostained only superficial keratinized epithelial layers in 11 cases, and also intermediate spinous layers in 18 cases. Cytokeratin 14 was expressed in all epithelial layers of 31 cases, in two cases its expression was in the basal and intermediate layers, and one case was negative. Cytokeratin 13 immunostained 26 cases and was negative in eight cases. In these eight cases, CK13 was apparently replaced by CK16. Cytokeratin 16, besides these eight cases, was also expressed in the spinous intermediate layers of a further eight cases. The remaining CKs tested were all negative. No relation between the degree of dysplasia and the CK expression was noted.Conclusions:  Cytokeratin expression in AC is different from that of normal oral mucosa, and is not related to the degree of dysplasia.