bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification
Authors: Lin Shu‐Hui , Su Tzu‐Cheng , Huang Shuo Jie , Jen Chun‐Ping 
Published: January 2024

AbstractMetastasis remains a significant cause to cancer‐related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer‐based binding, thereby enhancing capture efficiency and specificity. The microchip features interdigitated electrodes made of indium‐tin‐oxide that generate a nonuniform electric field to manipulate CTCs. Following three chip design, scenarios were investigated: (A) bare glass surface, (B) glass modified with gold nanoparticles (AuNPs) only, and (C) glass modified with both AuNPs and aptamers. Experimental results demonstrate that AuNPs significantly enhance capture efficiency under DEP, with scenarios (B) and (C) exhibiting similar performance. Notably, scenario (C) stands out as aptamer‐functionalized surfaces resisting fluid shear forces, achieving CTCs retention even after electric field deactivation. Additionally, an innovative reverse pumping method mitigates inlet clogging, enhancing experimental efficiency. This research offers valuable insights into optimizing surface modifications and understanding key factors influencing cell capture, contributing to the development of efficient cell manipulation techniques with potential applications in cancer research and personalized treatment options.
Application of model updating to a large‐scale hybrid simulation
Authors: Cheng Mao , Ruiz Maria Camila Lopez , Becker Tracy C. 
Published: January 2024

AbstractModel updating can enhance hybrid simulation by utilizing the experimental data from the physically tested substructure to update the parameters of like‐components in the numerical substructure throughout the test, improving the overall accuracy and reducing the extent of the experimental setup. Identifying and updating parameters can be challenging, especially when coupling between degrees of freedom (DOF) must be considered or the specimen experiences loading scenarios which result in newly observed behavior. To explore the performance of model updating under these challenging conditions, a large‐scale hybrid simulation was conducted using a model of a major toll bridge with seismic isolation lead rubber bearings (LRB). One LRB is physically tested considering axial, shear, and rotational loading, while the remainder of the bearings are simulated and updated with a phenomenological model within the numerical substructure. A weighted adaptive constrained unscented Kalman filter is applied as the online model updating algorithm. The study explored the effect of learning over different loading patterns, the selection of initial model parameters, and the selection of the physically tested substructure. The improvement of numerical model hysteresis performance accuracy of the force prediction demonstrates the benefits of model updating in large‐scale hybrid simulation.
Seismic response and capacity of inelastic acceleration‐sensitive nonstructural elements subjected to building floor motions
Authors: Magliulo Gennaro , D'Angela Danilo 
Published: January 2024

AbstractThe paper investigates the seismic response of nonstructural elements (NEs), focusing on acceleration‐sensitive components housed in buildings, modelled as inelastic Ibarra–Medina–Krawinkler (SDOF) systems. Incremental dynamic analysis (IDA) is carried out considering (a) representative suites of building floor motions (real loading histories recorded within reinforced concrete (RC) buildings and table testing protocol inputs) and (b) a wide range of NE models (with elastic frequencies ranging within 1–9 Hz). The Ibarra‐Medina‐Krawinkler (IMK) model was implemented in OpenSees, defining the key modeling parameters according to the formulations provided by Lignos and Krawinkler. Both IDA curves and component (acceleration) amplification factor (CAF) are characterized, also considering statistical measures. The seismic capacity of the investigated NEs is estimated through fragility curves, accounting for five incremental damage states (DSs). The fragility parameters are correlated with the frequency of the NE models, and (statistical‐based) closed‐form capacity criteria are provided. The study provides a robust technical and scientific methodological framework for assessing the seismic capacity of NEs that can be modeled by inelastic SDOF systems. The findings have a potential major impact on both research and practice, enriching scientific knowledge and providing useful applicative tools. In particular, quantitative response and capacity measures are supplied, and the developed capacity criteria can be particularly useful for expeditious but reliable design and assessment, as well as for comparison purposes.
Piecewise circular interface construction using height functions
Authors: Maity Ram Kumar , Sundararajan T. , Velusamy K. 
Published: January 2024

AbstractA piecewise circular interface construction (PCIC) method is described, where height functions based curvature estimates are directly utilised for accurate interface reconstruction under the framework of volume of fluid method. The present work is an attempt to develop a robust and accurate higher order interface reconstruction algorithm that is capable of accurate simulation of surface tension dominated flows. The proposed hybrid method (H‐PCIC) is thus able to take advantage of merits of both PCIC and HF methods, achieving at least second order convergence with respect to both interface reconstruction and curvature computation. This is in addition to the significantly superior quality of the reconstructed interface with respect to PLIC methods. This seamless blending of the HF and PCIC quantities is enabled by c0‐correction procedures applied to base PLIC and initial PCIC steps. More recent variants of the height function method with variable stencil size are used for calculation of radius of curvature. The capability of this proposed method towards simulation of flow problems within a well‐balanced two‐phase solver is established with help of multiple complex two‐phase flow problems. This validation exercise also demonstrates the capability of PCIC class of methods towards solutions of two‐phase flows with intricate physics.
Acute, subacute, and sub‐chronic toxicity evaluation of Bletilla formosana tubers in rats
Authors: Wang Yongchao , Zhang Jian , Zhao Tianrui , Cao Jianxin , Khan Afsar , Wang Zhengxuan , Liu Yaping , Cheng Guiguang 
Published: January 2024

AbstractBletilla formosana (Hayata) Schltr. is an important economic medicinal and edible plant in China. Its tubers (BFTs) are traditionally consumed as a beverage and canned foods for promoting human health. In this paper, the nutrients, the contents of total phenol, flavonoids, and polysaccharides, and the safety and toxicity of BFT are determined. The chemical analysis reported that BFT is rich in proteins, polysaccharides, polyphenols, flavonoids, and trace elements. The acute (14 days), subacute (28 days), and sub‐chronic (90 days) toxicities of BFT were evaluated for the first time in female and male rats. An acute toxicity study showed that the female rats by single oral administrations of BFT with 10,000 mg/kg body weight (BW) did not have behavioral changes and mortality, indicating that 50% lethal dose (LD50) of BFT was over 10,000 mg/kg BW. Similarly, BFT at 5000 mg/kg BW for consecutive 28 and 90 days in rats was also not toxic for rats after the analysis of physiological and biochemical parameters. Additionally, there are no significant differences on the histopathological changes of the heart, liver, spleen, lung, kidney, ovary, or testis between the control and BFT groups. Our findings revealed that BFT with concentration of less than 5000 mg/kg BW are safe for the female or male rats, indicating that BFT could be regarded as a safe food source.
From co‐regulation to self‐regulation: Maternal soothing strategies and self‐efficacy in relation to maternal reports of infant regulation at 3 and 7 months
Authors: Kiel Natalie , Samdan Gizem , Wienke Annika S. , Reinelt Tilman , Pauen Sabina , Mathes Birgit , Herzmann Charlotte 
Published: January 2024

AbstractThis study, conducted in Germany, examines the role of maternal soothing strategies to explain the association of maternal self‐efficacy with infant regulation (crying and sleeping behavior). Questionnaire data of 150 mothers, living in Germany, with mixed ethnic and educational backgrounds were collected when infants were 3 and 7 months old. Two types of maternal soothing strategies were distinguished: close soothing, involving close physical and emotional contact, and distant soothing, involving physical and emotional distancing from the infant. A cross‐sectional SEM at 3 months indicated that maternal self‐efficacy is associated with reported infant regulation through distant soothing strategies. Low maternal self‐efficacy was associated with frequent maternal use of distant soothing, which in turn was related to reported infant regulation problems, that is, non‐soothability and greater crying frequency. Frequent use of close soothing was associated with reported infant sleeping behavior, that is, frequent night‐time awakenings. A longitudinal SEM further indicated that the effects of close soothing persisted at least until the infants' age of 7 months. The study showed how low maternal self‐efficacy, increased use of distant soothing, and reported early infant regulation problems are intertwined and that, due to their persisting positive effect on infant soothability, close soothing better supports infant development.
Morphology and distribution of sensilla on antennae and mouthparts of the adult bruchid beetles, Bruchidius coreanus (Coleoptera: Bruchidae)
Authors: Peng Qi‐Yan , Xie Ming‐Hui , Pan Xiu‐Kui , Li You , Gao Lei , Xu Fangling , Wu Cheng‐Xu , Yang Mao‐Fa 
Published: January 2024

AbstractBruchidius coreanus is a serious pest on Gleditsia sinensis Lam during seed storage, causing significant losses to their yield in southwest China. To gain insight into their behavioral mechanisms, the external morphology, ultrastructure, and distribution of sensilla on antennae, maxillary palps, and labial palps of both male and female B. coreanus were observed using a scanning electron microscope. The results revealed that both male and female adults had serrated antennae comprising a scape, a pedicel, and nine flagellomeres (F1–F9). There were eight types and seven subtypes of antenna sensilla observed in both sexes, including Böhm sensilla (BS), two subtypes of sensilla chaetica (SC1 and SC2), two subtypes of sensilla trichodea (ST1 and ST2), three subtypes of sensilla basiconica (SB1, SB2, and SB3), sensilla auricillica (SA), sensilla styloconicum (SS), capitate pegs (CP), and sensilla cavity (SCa). The average length of BS and ST (ST1 and ST2) showed significant differences between males and females. Furthermore, the number of SC (SC1 and SC2), ST1, and SCa differed significantly between the sexes. Four types of sensilla were found on the maxillary palps and labial palps, with the length of ST on these palps significantly differing between males and females. Additionally, SS on male labial palps was significantly longer than in females. The number of SC significantly differed between the male and female maxillary palps and labial palps, while ST and SS showed significant differences in the maxillary palps. These findings will contribute to further electrophysiological recording and behavioral research.Research Highlights
The external morphology and distribution of various sensilla on the antennae, maxillary palps, and labial palps of Bruchidius coreanus were described.
Eight types and seven subtypes of antenna sensilla were observed on the antennae, while four types of sensilla were observed on the maxillary palps and labial palps.
The capitate pegs were found exclusively on the antennae of female B. coreanus.
Renal replacement therapy prior to liver transplant and inpatient mortality in patients without advanced kidney disease: A nationwide study
Authors: Ali Hassam , Moond Vishali , Lawson Cameron , Budh Deepa , Ohri Ritika , Patel Pratik , Jun Wong Yu , Rodriguez‐Zarate Eduardo , Mohan Babu P. 
Published: January 2024

AbstractBackground and AimThe utility of renal replacement therapy (RRT) before liver transplant (LT) in patients without end‐stage renal disease (ESRD) or advanced chronic kidney disease (CKD‐IV/V) is debatable and lacks data support. We aimed to evaluate the impact of RRT on patients undergoing LT.MethodsWe used the National Readmission Database (2016–2019) to identify all index hospitalizations undergoing RRT before LT (cases). A matched comparison cohort of similar hospitalizations without RRT before LT was identified (controls) after 1:1 propensity score matching for age, gender, and available comorbidities.ResultsWe matched 364 cases (RRT before LT) to 364 controls (LT without prior RRT). There was no statistical difference in all‐cause inpatient mortality (4.9% vs 3.6% P = 0.4). A significantly greater proportion of cases were associated with ICU admission (40.7% vs 17.0%, P < 0.001) and RRT requirement post LT (100% vs 17%, P < 0.001). There was no difference in 30‐ (hazard ratio [HR] 1.1, 0.4–2.6), 60‐ (HR 0.9, 0.4–1.8), or 90‐day (HR 0.8, 0.4–1.6) inpatient mortality between the groups. Also, 180‐day survival estimates were comparable (P = 0.5). The results were similar in patients with no chronic kidney disease (CKD) and CKD‐III.ConclusionRRT prior to LT, in patients without advanced CKD or ESRD, was associated with greater instances of ICU stay and need for future RRT. Also, 30‐, 60‐, and 90‐day inpatient mortality rates were similar, and 180‐day survival estimates were comparable.
Microanatomy and ultrastructure of the nervous system of adult Renicola parvicaudatus (Digenea: Renicolidae)
Authors: Denisova Sofia A. , Shchenkov Sergei V. , Lebedenkov Vladimir V. 
Published: January 2024

AbstractThe digenean complex life cycle includes various morphological forms with different locomotory and behavioral activities, and the functional specialization of their nervous system is of importance for the transmission of these parasites. Adult digeneans acquire many adaptive features associated with the final settlement in a vertebrate host. Our study describes the general morphology and ultrastructure of the nervous system of the adult renicolid digenean Renicola parvicaudatus parasitizing the renal tubules of herring gulls. Using immunocytochemical and electron microscopic methods, we identified the distinctive characteristics of ganglia and synapses in the studied species. A comparative analysis of the organization of the nervous system of adult individuals and their continuously‐swimming stylet cercariae revealed a number of stage‐related differences in the composition of ganglia, the distribution of serotonin‐ and FMRFamide‐immunoreactive neurons, the cytomorphology of neuron somata and free sensory endings. Thus, in adults, the presence of FMRFamide‐positive neuron somata, accessory muscle bundles in the ganglionic cortex, and eight types of neuronal vesicles was detected, but no glia‐like elements were identified. Their neurons are characterized by a larger volume of cytoplasm and also show greater ultrastructural diversity. Although the sensory papillae of adults do not vary in their external morphology as much as those of larvae, their sensory bulbs are more diverse in cytomorphology. Following our previous data on the “support” cell processes related to various tissues of the larvae and considered as glia‐like structures, we also briefly present the identified features of the parenchyma, attachment organs and excretory system of adult individuals. The excretory system of adult R. parvicaudatus is characterized by the presence of unique terminal cells with several flame tufts, which are not typical either for the larvae of this species or for other digeneans studied so far. We also used molecular phylogenetic analysis to clarify species identification.
Liver T1 Mapping Derived From Cardiac Magnetic Resonance Imaging: A Potential Prognostic Marker in Idiopathic Dilated Cardiomyopathy
Authors: Wang Jiaqi , Diao Yike , Xu Yuanwei , Guo Jiajun , Li Weihao , Li Yangjie , Wan Ke , Sun Jiayu , Han Yuchi , Chen Yucheng 
Published: January 2024

BackgroundHepatic alterations are common aftereffects of heart failure (HF) and ventricular dysfunction. The prognostic value of liver injury markers derived from cardiac MRI studies in nonischemic dilated cardiomyopathy (DCM) patients is unclear.PurposeEvaluate the prognostic performance of liver injury markers derived from cardiac MRI studies in DCM patients.Study TypeProspective.PopulationThree hundred fifty‐six consecutive DCM patients diagnosed according to ESC guidelines (age 48.7 ± 14.2 years, males 72.6%).Field Strength/SequenceSteady‐state free precession, modified Look‐Locker inversion recovery T1 mapping and phase sensitive inversion recovery late gadolinium enhancement (LGE) sequences at 3 T.AssessmentClinical characteristics, conventional MRI parameters (ventricular volumes, function, mass), native myocardial and liver T1, liver extracellular volume (ECV), and myocardial LGE presence were assessed. Patients were followed up for a median duration of 48.3 months (interquartile range 42.0–69.9 months). Primary endpoints included HF death, sudden cardiac death, heart transplantation, and HF readmission; secondary endpoints included HF death, sudden cardiac death, and heart transplantation. Models were developed to predict endpoints and the incremental value of including liver parameters assessed.Statistical TestsOptimal cut‐off value was determined using receiver operating characteristic curve and Youden method. Survival analysis was performed using Kaplan–Meier and Cox proportional hazard. Discriminative power of models was compared using net reclassification improvement and integrated discriminatory index. P value <0.05 was considered statistically significant.Results47.2% patients reached primary endpoints; 25.8% patients reached secondary endpoints. Patients with elevated liver ECV (cut‐off 34.4%) had significantly higher risk reaching primary and secondary endpoints. Cox regression showed liver ECV was an independent prognostic predictor, and showed independent prognostic value for primary endpoints and long‐term HF readmission compared to conventional clinical and cardiac MRI parameters.Data ConclusionsLiver ECV is an independent prognostic predictor and may serve as an innovative approach for risk stratification for DCM.Evidence Level1Technical EfficacyStage 2
Effect of inoculation with arbuscular mycorrhizal fungi on the energy metabolism of selenite‐rich amaranth
Authors: Mu Mao , Lu Yunmei , Liao Haolin , Huang Renhua 
Published: January 2024

AbstractA series of pot trials were undertaken to examine the impact of four arbuscular mycorrhizal fungi (AMF), namely Glomus mosseae (G.m), Glomus etunicatum (G.e), Corymbiglomus tortuosum (C.t), and the combined application of Glomus etunicatum and Corymbiglomus tortuosum (G.e + C.t), on the energy metabolism of amaranth plants grown in soil enriched with selenite at a concentration of 0.5 mg kg−1. The inoculation of four AMFs resulted in an increase in both amaranth biomass and selenium (Se) content in leaves. The activities of phosphoglucose isomerase (PGI) and glucose‐6‐phosphate dehydrogenase + 6‐phosphogluconate dehydrogenase were observed to decrease when AMFs were inoculated, as compared with the absence of AMF inoculation. The inoculation with G.m, C.t, and G.e + C.t resulted in an increase in succinate dehydrogenase activity; however, the inoculation with G.m, G.e, and G.e + C.t led to an increase in ascorbate oxidase activity. Furthermore, the inoculation of all four AMFs resulted in an increase in cytochrome c oxidase activity and the concentrations of oxidized coenzyme I (NAD) and reduced coenzyme I (NADH). The polyphenol oxidase activity of amaranth plants increased when inoculated with G.m and G.e, whereas it decreased when inoculated with C.t and G.e + C.t. Furthermore, the application of all four AMF treatments resulted in a reduction in adenosine triphosphate (ATP) levels and energy charge. It was worth mentioning that there was a clear inverse relationship between the energy charge and the biomass, Se concentration in the leaves. The findings presented in this research indicated that AMF may have an impact on energy metabolism and ultimately the biomass of amaranth by influencing the uptake of Se.
Clinical and Anatomical Variation During Assessment of Maximum Glottic Angle
Authors: DeChance Daniel , Frank Ethan , Dehom Salem , Watson WayAnne , Simmons Ethan , Krishna Priya D. , Crawley Brianna 
Published: January 2024

IntroductionQuantitative measurement and analysis of glottic abduction is used to assess laryngeal function and success of interventions; however, the consistency of measurement over time has not been established. This study assesses the consistency of glottic abduction measurements across visits in healthy patients and anatomic factors impacting these measurements.MethodsReview of patients with two sequential flexible stroboscopic exams over seven months from 2019–2022. Images of maximal glottic abduction were captured and uploaded into and measured with ImageJ. Cadaver heads were used to assess the impact of visualization angles on glottic measurements with a monofilament inserted into the supraglottis of each cadaver as a point of reference. Comparisons were done with a paired T‐test, T‐test, or Mann–Whitney U test as appropriate.ResultsFifty‐nine patients and twenty‐six cadaveric exams were included. Absolute change in maximum glottic abduction angle (MGAA) was 6.90° (95% CI [5.36°, 8.42°]; p < 0.05). There were no significant differences in change in MGAA by gender or age. Twenty percent of patients had a change of at least 25% in their MGAA between visits. Absolute differences in glottic angle between nasal side for cadaveric measurements was 4.77 ± 4.59° (p < 0.005)—2.22° less than the change in MGAA seen over time (p = 0.185).ConclusionMaximal glottic abduction angles varied significantly between visits. Factors considered to be contributing to the differences include different viewing windows between examinations due to the position and angulation of the laryngoscope and changes in patient positioning, intra‐ and inter‐rater variations in measurement, and patient effort.Level of EvidenceN/a Laryngoscope, 134:2793–2798, 2024