bluuebunny/crossref_metadata_2025_split huggingface dataset:
Karakteristik Rasm dalam Manuskrip Mushḥaf Al-Qur’an KH. Abdul Hamid Chasbullah
Authors: Fithrotul Aini Adrika , Khanin Mahfudzoh Rif’atul , Noorhidayati Salamah 
Published: February 2024

One of the Qur'an manuscripts from the 20th century is the written version (mushḥaf) KH. Abdul Hamid Chasbullah. The mushḥaf is evidence of the historical heritage of the ulama of Nusantara who have special attention in the field of copying the Qur'an manuscripts. In this study, the author will examine the general description of the mushḥaf and the characteristics of the rasm by referring to the rules of Sheikh Jalal Al-din Al-Suyuthi in the book Al-Itqan fi Ulum Qur'an. The method that the author uses is qualitative research that leads to library research, by using observation tools, interviews, and documentation of the manuscript. The findings of this study are first, this mushḥaf is kept intact (not scattered) although some sheets have been lost and eaten by termites. This 32.3 x 21 x 7.5(cm) mushḥaf was written for the personal use of the copyist. It can be seen that there aren’t illustration and illumination aspects. Second, from the analysis of surah Al-Baqarah juz 2, the copying of this mushḥaf still adheres to the basics of mixed rules between the rasm utsmani and imla'i. Likewise, in terms of qira'at, this mushḥaf mostly uses the history of Imam Hafs 'an Ashim, but some words use mixed history.
Wake characteristics of wind-sand cylindrical flow under different turbulence intensity
Authors: Liang ChangPing , Lu Bo , Wang Qing , Guo XingDuo 
Published: February 2024

Abstract

Wind-sand environment has a great influence on wind turbine performance. In order to further investigate the influence of turbulence on wind turbine wake characteristics in wind-sand environment, the gas-solid two-phase numerical simulation was carried out by using SST k-ω turbulence model and MPPICFoam solver based on OpenFOAM to solve the problem of flow around a cylinder. The variation of aerodynamic load on the cylinder surface of clean flow field and wind-sand flow field under different turbulence is compared, and the frequency of vortex shedding is studied by spectrum analysis. Secondly, the particle distribution and the evolution process of the wind-sand flow field are studied. Finally, different wake velocity sections are selected to analyze the influence of different turbulence intensity on the wake velocity of the wind-sand flow field. The results show that wind-blown sand reduces the frequency of wake vortex shedding, while turbulence increases the frequency of wake vortex shedding and reduces the scale of wake vortex. In addition, the wind-sand flow field accelerates in the far wake region, and the increase of turbulence strengthens this phenomenon.
Association between self-perception of genital image in patients diagnosed with endometriosis and chronic pelvic pain and healthy women.
Authors: Palomares Paula Navarro , Boyero Noemí Álvarez , Mohedo Esther Díaz , Herrera María José Suárez 
Published: February 2024

Abstract

Introduction: Endometriosis is a pathology that carries a high social stigma and a factor in the quality of life of the sufferer. Its etiology is unknown and treatment includes surgical and conservative measures, including hormonal suppression.
Objectives: The main objective of the study is to demonstrate a distortion of the genital image in patients diagnosed with endometriosis with chronic pelvic pain, the secondary objective is to associate the distortion of the genital image with the quality of life.
Methodology: A cross-sectional study of cases (women with endometriosis and CPP) and controls (healthy women) were carried out with a total of 284 women over 18 years of age.
Results: Statistically significant differences were obtained in favor of a lower perception of genital self-image and quality of life (for the physical component), in women with endometriosis and CPP, compared to healthy women.
Conclusion: More studies with medium-long term follow-up are needed to be able to make a correct understanding of the process and offer an adequate multidisciplinary approach, aiming at the reconciliation of the idea that the body and the mind are one.
KRAS silencing impacts chromatin organization and transcriptional activity in colorectal cancer cells
Authors: Martins Flávia , Machado Ana Luísa , Ribeiro Andreia , Oliveira Susana Mendonça , Carvalho Joana , Matthiesen Rune , Backman Vadim , Velho Sérgia 
Published: February 2024

Abstract
Preclinical and clinical data have highlighted the challenges in targeting KRAS mutant tumors, revealing that cancer cells initially sensitive to treatment circumvent KRAS dependence and become tolerant. However, the exact mechanisms governing the transition from a drug-sensitive to a drug-tolerant state remain unclear. Herein, we used 3D culture models of mutant KRAS colorectal cancer cells with distinct KRAS dependencies to show that sensitive and resistant cells undergo distinct chromatin and transcriptional adaptations upon acute KRAS loss. KRAS-silenced sensitive cells presented G0/G1 cell cycle arrest and exhibited a transcriptional signature characteristic of quiescent cells. Moreover, proteomic profiling underscored common biological processes up-regulated in sensitive cells, including nucleosome assembly, gene expression regulation, and mRNA splicing. A detailed analysis of chromatin features revealed that sensitive cells altered eu/heterochromatin states and gained TADs after KRAS silencing whereas the eu/heterochromatin states of resistant cells remained unchanged, and the number of TADs decreased. Strikingly, only KRAS-silenced sensitive cells displayed alterations in how chromatin organizes into packing domains, displaying a lower domain packing scaling. Chromatin packing scaling regulates the interaction between macromolecular complexes and DNA, thus controlling transcriptional malleability and plasticity. Accordingly, although KRAS-silenced sensitive and resistant cells displayed a transcriptomic profile distinct from their controls, only sensitive cells presented higher variability over time, thus suggesting higher transcriptional plasticity. Overall, our findings provide compelling evidence supporting chromatin 3D reorganization and transcriptional variability in KRAS-silenced sensitive cells. This epigenetic mechanism is likely to underlie the remarkable ability of cancer cells to adapt, persist, and sustain malignancy without oncogenic KRAS.
Power Generation and Water Conservation Studies in Sudan Canals Using PV Panels
Authors: Abaas Sheikhaldeen N. S. , Naik M. T. 
Published: February 2024

Abstract
The construction of the Grand Ethiopian Renaissance Dam has raised concerns about potential water and energy shortages in Sudan. To address this issue, a scientific paper has proposed installing solar panels on the Al-Jazeera and Al-Mangal irrigation canals in Sudan. This approach aims to prevent clean water evaporation and provide electricity to people living along the canals without the need for extensive transmission lines. The study aims to determine the optimal coverage area for solar panels which will minimize evaporation volume and maximize energy generation without compromising water quality. the installation of solar panels on the two main canals has a potential to generate around 27,000 MW-hr/day of electricity and conserve approximately 70 million mm3 of water per year. The results of this study contribute to the growing body of knowledge supporting the feasibility of using solar panels in developing countries, and emphasize the importance of sustainable energy and water management.
Pedunculopontine-stimulation obstructs hippocampal theta rhythm and halts movement
Authors: Berg Rune , Komi Salif , Dmytriyeva Oksana , Houser Grace , Bonfils Madelaine , Kaur Japreet 
Published: February 2024

Abstract

It is known that movement arrest can be induced by optogenetic stimulation of a brainstem nucleus, the pedunculopontine nucleus (PPN)1–6. This evoked arrest appears conspicuously similar to the freezing behavior often seen as a fear response. Nevertheless, the behavior could also represent a global halting mechanism for movement. This introduces two possible roles of this nucleus: A hub for orchestrating fear-related responses or an omnipotent halting mechanism devoid of emotional components that could, in principle, encompass brain activities other than movement per se. We seek to understand this phenomenon better by engaging the distinct electrical brain activity, the hippocampal theta rhythm7,8. It is prominent during locomotor activity in rodents9–13 and immobile yet vigilant states like freezing14–18. We recorded the hippocampal brain rhythm before and during movement arrest induced by optogenetic stimulation of the PPN in rats. The motor arrest was associated with a clear obstruction of the ongoing theta activity. The timescale of movement arrest was less than 200 ms, similar to the obstruction of the theta rhythm. Since anxiety, fear, and behavioral freezing are associated with hippocampal theta rhythm, which we did not see during PPN stimulation, we suggest that induced motor arrest occurs without an associated emotional component.
A novel pillar-layered MOF with the urea linkers: A capable catalyst for the synthesis of new 1,8-naphthyridines via the anomeric-based oxidation
Authors: Beiranvand Masoumeh , Habibi Davood , Khodakarami Hosein 
Published: February 2024

Abstract

Metal-based catalysts play an essential role in organic chemistry and the chemical industry. In this research, a pillar-layered metal-organic framework (MOF) with the urea linkers, namely Basu-HDI, as a novel and efficient heterogeneous catalyst was designed and successfully synthesized. Various techniques such as FT-IR, EDX, elemental mapping, SEM, XRD, BET, and TGA/DTA studied its structure and morphology. Then, we investigated the synthesis of new 1,8-naphthy-ridines utilizing Basu-HDI in mild conditions via a one‐pot three‐component tandem Knoevenagel/ Michael/cyclization/anomeric-based oxidation reaction. Final products were achieved by anomeric-based oxidation without employing an oxidation agent. Remarkably, this tandem process gave a good range of new 1,8-naphthyridines with high yields in a short reaction time. The pure products were confirmed by FT-IR, 1H NMR, 13C NMR, and mass spectrometry techniques. Moreover, the introduced catalyst showed good efficiency and stability and can be reused four times without significantly reducing efficiency.
SARS-CoV-2 RNA-dependent RNA Polymerase is Linked to Mitochondrial Dysfunction in Mouse Heart
Authors: Naka Kazuhito , Ooshima Akira , Sakaguchi Takemasa , Sotomaru Yusuke , Koike Kanae , Sakamoto Naoya , Ishikawa Tomo-o , Kim Seong-Jin , Sawa Masaaki 
Published: February 2024

Abstract
Little is known about specific viral factors responsible for the pathogenesis and pathophysiology of long COVID1-5. Here we describe a conditional knock-in (cKI) mouse strain inducibly expressing Nsp12, an essential component of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp). Induction of Nsp12 translation was dependent on co-treatment with inhibitors of the integrated stress response in vitro and in vivo. We show that Nsp12 has a biologically significant link to mitochondrial dysfunction in vivo. In vitro, ectopic Nsp12 expression suppressed mitochondrial function in primary lung epithelial cells isolated from Nsp12 cKI mice. This functionality was physiologically relevant because, although ectopic Nsp12 expression in mouse lungs did not induce pneumonia, it did decrease mitochondrial activity in the hearts of Nsp12 cKI mice over the short and long terms. Administration in vivo of an RdRp inhibitor, EIDD-2801, restored mitochondrial function in cardiomyocytes of Nsp12 cKI mice. Our data demonstrate that SARS-CoV-2 RdRp activity in the lungs leads to cardiac mitochondrial dysfunction in vivo, generating a phenotype resembling aspects of long COVID in humans. Therapeutic targeting of SARS-CoV-2 RdRp may thus represent a novel means of preventing or mitigating intense fatigue and/or myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)-like disease caused by mitochondrial dysfunction in long COVID patients.
Genomic Profiling of Insecticide Resistance in Malaria Vectors: Insights into Molecular Mechanisms.
Authors: Ingham Victoria , Nagi Sanjay 
Published: February 2024

Abstract
Malaria control faces challenges from widespread insecticide resistance in major Anopheles species. This study, employing a cross-species approach, integrates RNA-Sequencing, whole-genome sequencing, and microarray data to elucidate drivers of insecticide resistance in Anopheles gambiae complex and An. funestus. Findings show an inverse relationship between genetic diversity and gene expression, with highly expressed genes experiencing stronger purifying selection. These genes cluster physically in the genome, revealing potential coordinated regulation. We identified known and novel candidate insecticide resistance genes, enriched in metabolic, cuticular, and behavioural functions. We also present AnoExpress, a Python package, and an online interface for user-friendly exploration of resistance candidate expression. Despite millions of years of speciation, convergent gene expression responses to insecticidal selection pressures are observed across Anopheles species, providing crucial insights for malaria vector control. This study culminates in a rich dataset that allows us to understand molecular mechanisms, better enabling us to combat insecticide resistance effectively.
Experimental tests strongly challenge the evidence of a healthy human blood microbiome
Authors: Gloria Leandro Di , Baldi Simone , Curini Lavinia , Bertorello Sara , Nannini Giulia , Cei Francesco , Niccolai Elena , Ramazzotti Matteo , Amedei Amedeo 
Published: February 2024

Abstract

The advent of next-generation sequencing (NGS) technologies has made it doable to investigate the microbial communities in various environments, including all the different human body districts. Hence, the previously established belief of several body sites’ sterilities, including human blood, has now been challenged. However, the metagenomics investigation of habitats with an anticipated low microbial biomass may be susceptible to many and dangerous misinterpretations. Here, we critically evaluate the results of 16S targeted amplicon sequencing performed on the total DNA collected from healthy donors’ blood samples in the light of specific negative controls aimed at addressing potential bias in order to supply the research in this area with trustworthy data. Our negative controls, were prepared by inflating the initial DNA quantity through sequences that can be recognised and subsequently discarded. We found that only three organisms resulted sporadically present among the samples, mostly attributable to bacteria ubiquitous in laboratory reagents. Despite we are not fully able to confirm or deny the existence of healthy blood microbiota, our results strongly suggest that living bacteria, or at least their residual DNA sequences, are not a common and peculiar feature of human blood in healthy people. Finally, our study poses relevant questions on the design of controls in this peculiar research topic in order to avoid spurious results that appear to contaminate current high throughput research.
The clinical efficacy of pegfilgrastim to prevent febrile neutropenia in breast cancer patients receiving docetaxel- cyclophosphamide chemotherapy
Authors: Banno Hirona , Fujii Kimihito , Ohashi Wataru , Saito Masayuki , Ito Yukie , Ido Mirai , Goto Manami , Ando Takahito , Mouri Yukako , Kousaka Junko , Imai Tsuneo , Nakano Shogo 
Published: February 2024

Abstract
Background and aim
: For early-stage breast cancer patients, the myelosuppressive chemotherapy such as docetaxel and cyclophosphamide (TC) chemotherapy are frequently introduced as an adjuvant treatment postoperatively. This regimen is known to develop febrile neutropenia (FN) commonly, therefore a newly developed granulocyte colony-stimulating factor (G-CSF), pegfilgrastim, play an important role in preventing the occurrence of FN. In this study, the clinical advantage of pegfilgrastim during the TC chemotherapy was evaluated by the comparison with the conventional filgrastim.
Patients and method
: A total of 85 patients with stage I or II breast cancer who received TC chemotherapy were divided into the 2 groups, which included: the one that consisted of the patients experienced prophylactic pegfilgrastim administration (named as PEG(+)); the two that consisted of the patients received chemotherapy only with contemporary G-CSF agent, filgrastim that was administered when they suffered from FN or severe neutropenia (named as PEG(-)). This study method was drawn up to evaluate the effectiveness of pegfilgrastim for the prevention of FN and keeping the high relative dose intensity (RDI) (1st endpoint) and to explore whether pegfilgrastim usage might affect the disease-free survival of the patients (2nd endpoint).
Results
The number of the patients that were diagnosed as Grade 3 to 4 “neutrophil count discount” according to common terminology criteria for adverse events version 5.0 was 21 and 6 in PEG(-) and PEG(+), respectively (P = 0.0238). The odds ratio for the onset of “neutrophil count decreased” ranging with Grade 3 to 4 of PEG(+) compared to that of PEG(-) was 0.1143 (95% confidence interval, 0.0175–0.7446). The significant difference of disease-free survival rates of each could not reach the significant level, because the number of events were small.
Conclusion
The administration of pegfilgrastim significantly reduced the risk of development of FN with acceptable adverse events. The chemotherapy RDI of the patients who had prophylactic use of pegfilgrastim was significantly higher than that of the patients who were administered conventional filgrastim by physician’s decision. In this study, we could verify the clinical effectiveness of pegfilgrastim on the patients receiving TC chemotherapy.
A novel Variant in GAS2 Is Associated with Non-Syndromic Autosomal Dominant Hearing Impairment in a Chinese Family
Authors: Zhang Luping , Zheng Danya , Xu Lian , Wang Han , Zhang Shuqiang , Shi Jianhua , Jin Nana 
Published: February 2024

Abstract
Mutation in the GAS2 gene, encoding growth arrest-specific protein 2 (GAS2), causes the disorganization and destabilization of microtubule bundles in supporting cells of the cochlear duct, leading to hearing loss in vivo. The expression and function of GAS2 in cochlear duct is confirmed. However, the molecular mechanism of GAS2 mutant resulting in hearing loss is still unknown. Whole exome sequencing (WES) was employed to identify pathogenic variants. The reverse transcription-PCR was used to show the retention of GAS2 intron 6. The wild-type GAS2 and the truncated GAS2 (mimicking GAS2 variant) were constructed. The protein expression level and cellular localization of GAS2 were checked by Western blots and immunofluorescence staining. The cell apoptosis was assayed by Cell Counting Kit-8 and The DeadEnd™ Fluorometric TUNEL System. We identify a novel heterozygous variant in GAS2 (c.616-2A > G) as the only candidate mutation segregating with late-onset and progressive NSHL in a large dominant family by WES. The mutation causes the retention of intron 6 in mature mRNA and a C-terminally truncated protein (named as GAS2mu) due to an in-frame stop codon (TGA) at c.615 + 109–111 in intron 6. Mechanically, GAS2mu enhances its degradation by ubiquitin-proteasome and displays disorganized microtubule bundles. Additionally, GAS2mu further promotes cell apoptosis by upregulating the ratio of Bcl-xS/Bcl-xL, instead of p53-dependent as wild-type, indicating that GAS2mu acts as a toxic molecule to aggravate cell apoptosis. Our findings demonstrate that the novel variant of GAS2 promotes its protein degradation, microtubule disorganization and cell apoptosis, leading to hearing loss in variant carriers. This study expands the spectrum of GAS2 variants and elucidates the underlying pathogenic mechanisms, which provides a foundation for future investigation of new therapeutic strategies to prevent GAS2-associated progressive hearing loss.
Loss of heterozygosity in CCM2 cDNA revealing a structural variant causing multiple cerebral cavernous malformations
Authors: Riant Florence , Chaussenot Annabelle , Ayrignac xavier , Chatron Nicolas , Riolzyr Terry Granchon , Labauge Pierre , Elisabeth Tournier Lasserve 
Published: February 2024

Abstract

Loss-of-function variants in CCM1/KRIT1, CCM2/MGC4607 and CCM3/PDCD10 genes are identified in the vast majority of familial cases with multiple cerebral cavernous malformations. However, genomic DNA sequencing combined to large rearrangement screening fails to detect a pathogenic variant in 5% of the patients. We report a family with two affected members harboring multiple CCM lesions, one with severe hemorrhages and one asymptomatic. No causative variant was detected using genomic DNA sequencing, CNV detection analysis and RNA sequencing. However, a loss of heterozygosity in CCM2 was observed on cDNA sequences in one of the two affected members, which suggested that this locus might be involved. Whole genome identified a balanced structural variant on chromosome 7 with a breakpoint interrupting the CCM2 gene, preventing normal mRNA synthesis. These data underline the importance of WGS in undiagnosed patients with typical multiple CCM.
Axia: A Home-based Exercise-App with Patient-Education and Self-Management Features - An innovative therapeutic approach for Axial Spondyloarthritis. Results from a Pilot Study.
Authors: Strunz Patrick-Pascal , Maire Maxime Le , Heusinger Tobias , Klein Juliana , Labinsky Hannah , Fleischer Anna , Luetkens Karsten Sebastian , Possler Patricia , Gernert Michael , Leppich Robert , Schmieder Astrid , Hammel Ludwig , Schulz Evelin , Sperlich Billy , Froehlich Matthias , Schmalzing Marc 
Published: February 2024

Abstract
Background
Patients with axial spondyloarthritis (axSpA) benefit from regular home-based exercise (HbE). In spite of recommendations, a relevant proportion of German axSpA patients does not adhere to recommended HbE practices. To enhance HbE care, we developed the novel digital therapeutic (DTx) “Axia” compliant with the European medical device regulation (MDR). Axia offers a modern app-based HbE solution with patient educative content and further integrated features. A first patient survey involved axSpA patients who assessed Axia's efficacy, attractiveness, and functionality through a questionnaire.
Methods
A mixed-method online questionnaire with 38 items was administered to 37 axSpA volunteers after using Axia. Numeric rating scales (NRS) and likelihood scales were primarily used.
Results
Axia received a median rating of 5 out of 5 stars. On NRS scales, Axia scored a median of 9 for intuitiveness and design, and a median of 8 for entertainment. HbE frequency significantly increased from a median of 1 day/week to 6 days/week (p<0.001). Existing HbE practitioners also increased their frequency (median of 4 days/week before, 6 days/week with Axia, p<0.05). 64.9% reported improved range of motion, 43.2% reported reduced pain, and 93.6% enhanced disease-specific knowledge. All users recommended Axia to other patients.
Conclusion
Axia represents a promising DTx for axSpA, warranting further investigation in large randomized controlled interventional trials to establish its efficacy conclusively.