bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a prognostic Neutrophil Extracellular Traps related lncRNA signature for soft tissue sarcoma using machine learning
Authors: Liu Binfeng , He Shasha , Li Chenbei , Li Zhaoqi , Feng Chengyao , Wang Hua , Tu Chao , Li Zhihong 
Published: January 2024

BackgroundSoft tissue sarcoma (STS) is a highly heterogeneous musculoskeletal tumor with a significant impact on human health due to its high incidence and malignancy. Long non-coding RNA (lncRNA) and Neutrophil Extracellular Traps (NETs) have crucial roles in tumors. Herein, we aimed to develop a novel NETsLnc-related signature using machine learning algorithms for clinical decision-making in STS.MethodsWe applied 96 combined frameworks based on 10 different machine learning algorithms to develop a consensus signature for prognosis and therapy response prediction. Clinical characteristics, univariate and multivariate analysis, and receiver operating characteristic curve (ROC) analysis were used to evaluate the predictive performance of our models. Additionally, we explored the biological behavior, genomic patterns, and immune landscape of distinct NETsLnc groups. For patients with different NETsLnc scores, we provided information on immunotherapy responses, chemotherapy, and potential therapeutic agents to enhance the precision medicine of STS. Finally, the gene expression was validated through real-time quantitative PCR (RT-qPCR).ResultsUsing the weighted gene co-expression network analysis (WGCNA) algorithm, we identified NETsLncs. Subsequently, we constructed a prognostic NETsLnc signature with the highest mean c-index by combining machine learning algorithms. The NETsLnc-related features showed excellent and stable performance for survival prediction in STS. Patients in the low NETsLnc group, associated with improved prognosis, exhibited enhanced immune activity, immune infiltration, and tended toward an immunothermal phenotype with a potential immunotherapy response. Conversely, patients with a high NETsLnc score showed more frequent genomic alterations and demonstrated a better response to vincristine treatment. Furthermore, RT-qPCR confirmed abnormal expression of several signature lncRNAs in STS.ConclusionIn conclusion, the NETsLnc signature shows promise as a powerful approach for predicting the prognosis of STS. which not only deepens our understanding of STS but also opens avenues for more targeted and effective treatment strategies.
Causal role of immune cells in generalized anxiety disorder: Mendelian randomization study
Authors: Ma Zhen , Zhao Min , Zhao Huanghong , Qu Nan 
Published: January 2024

BackgroundGeneralized anxiety disorder (GAD) is a prevalent emotional disorder that has received relatively little attention regarding its immunological basis. Recent years have seen the widespread use of high-density genetic markers such as SNPs or CNVs for genotyping, as well as the advancement of genome-wide association studies (GWAS) technologies, which have facilitated the understanding of immunological mechanisms underlying several major psychiatric disorders. Despite these advancements, the immunological basis of GAD remains poorly understood. In light of this, we aimed to explore the causal relationship between immune cells and the disease through a Mendelian randomization study.MethodsThe summary information for GAD (Ncase=4,666, Ncontrol=337,577) was obtained from the FinnGen dataset. Summary statistics for the characterization of 731 immune cells, including morphological parameters (MP=32), median fluorescence intensity (MFI=389), absolute cells (AC=118), and relative cells (RC=192), were derived from the GWAS catalog. The study involved both forward MR analysis, with immune cell traits as the exposure and GAD as the outcome, and reverse MR analysis, with GAD as the exposure and immune cell traits as the outcome. We performed extensive sensitivity analyses to confirm the robustness, heterogeneity, and potential multi-biological effects of the study results. Also, to control for false positive results during multiple hypothesis testing, we adopted a false discovery rate (FDR) to control for statistical bias due to multiple comparisons.ResultsAfter FDR correction, GAD had no statistically significant effect on immunophenotypes. Several phenotypes with unadjusted low P-values are worth mentioning, including decreased PB/PC levels on B cells(β=-0.289, 95%CI=0.044~0.194, P=0.002), reduced PB/PC AC in GAD patients (β=-0.270, 95% CI=0.77~0.92, P=0.000), and diminished PB/PC on lymphocytes (β=-0.315, 95% CI=0.77~0.93, P=0.001). GAD also exerted a causal effect on CD27 on IgD-CD38br (β=-0.155,95%CI=0.78~0.94,P=0.002), CD20-%B cell (β= -0.105,95% CI=0.77~0.94, P=0.002), IgD-CD38br%lymphocyte(β=-0.305, 95%CI=0.79~0.95, P=0.002), FSC-A level on granulocytes (β=0.200, 95%CI=0.75~0.91, P=8.35×10−5), and CD4RA on TD CD4+(β=-0.150, 95% CI=0.82~1.02, P=0.099). Furthermore, Two lymphocyte subsets were identified to be significantly associated with GAD risk: CD24+ CD27+ B cell (OR=1.066,95%CI=1.04~1.10,P=1.237×10−5),CD28+CD4+T cell (OR=0.927, 95%CI=0.89~0.96, P=8.085×10−5).ConclusionThe study has shown the close association between immune cells and GAD through genetic methods, thereby offering direction for future clinical research.
Lactobacillus plantarum MH-301 as an effective adjuvant to isotretinoin in the treatment of acne vulgaris: a randomized and open-label trail
Authors: Liang Lili , Qi Xinyue , Jiang Xiaoke , Chen Tingtao , Dong Lina 
Published: January 2024

IntroductionAcne vulgaris is a common chronic inflammatory skin disease originating in the sebaceous gland units of the skin follicles. Isotretinoin is presently the primary choice for the treatment of acne vulgaris. However, it could induce several adverse reactions like diarrhea, cheilitis, headache, elevated triglyceride levels and risk of inflammatory bowel disease and depression. Hence, it is imperative to seek an alternative therapy.MethodsOne hundred five patients were randomly divided into 3 groups, and received a baseline treatment of oral doxycycline for the initial 4 weeks. Group I received isotretinoin oral for 12 weeks; Group P received oral Lactobacillus plantarum MH-301 treatment for 12 weeks; Group IP received combined treatment with oral probiotics and oral isotretinoin for 12 weeks. The number of skin lesions was recorded at 0, 4, 8, and 12 weeks during the treatment to compare the efficacy of each intervention, and skin and fecal samples were collected from patients at 12 weeks for high-throughput sequencing to explore the microbiota differences between various groups.ResultsOur results revealed that the combination of L. plantarum MH-301 with isotretinoin significantly reduced the number of skin lesions in patients compared to using L. plantarum MH-301 and isotretinoin alone (p &lt; 0.001). Additionally, skin microbiome High-throughput analysis indicated the restorative effects of L. plantarum MH-301 on skin microbial diversity while also observing a reduction in the main microbiota of skin lesions, Propionibacterium and Corynebacterium. Meanwhile, gut microbiome High-throughput analysis showed that it could regulate disorders of the intestinal microbiota and increased the abundance of probiotics such as Lactobacillus, Bifidobacterium, Coprococcus and Bacteroides genera.ConclusionIn conclusion, L. plantarum MH-301 could be used in combination with isotretinoin for optimal results in the treatment of acne vulgaris. The research conducted provides theoretical and data support for the adjuvant effect of L. plantarum in the treatment of acne vulgaris.Clinical Trial Registration[ClinicalTrials.gov], identifier (ChiCTR2200063499).
Vertical transfer and functional characterization of cotton seed core microbiome
Authors: Wu Chongdie , Zhang Xin , Fan Yongbin , Ye Jingyi , Dong Lingjun , Wang YuXiang , Ren YinZheng , Yong HongHong , Liu Ruina , Wang Aiying 
Published: January 2024

IntroductionMicrobiome within plant tissues is pivotal for co-evolution with host plants. This microbiome can colonize the plant, with potential transmission via seeds between parents and offspring, affecting seedling growth and host plant adaptability to the environment.MethodsWe employed 16S rRNA gene amplicon analysis to investigate the vertical distribution of core microbiome in cotton seeds across ecological niches [rhizosphere, root, stem, leaf, seed and seed-P (parental seed)] of the three cotton genotypes.ResultsThe findings demonstrated a significant decrease in microbiome diversity and network complexity from roots, stems, and leaves to seeds. The microenvironment exerted a more substantial influence on the microbiome structure of cotton than the genotypes. The core endophytic microorganisms in cotton seeds comprised 29 amplicon sequence variants (ASVs) affiliated with Acidimicrobiia, Alphaproteobacteria, Bacilli, Bacteroidia, Clostridia, Gammaproteobacteria, and unclassified_Proteobacteria. These vertically transmitted taxa are widely distributed in cotton plants. Through 16S rRNA gene-based function prediction analysis of the cotton microbiome, we preliminarily understood that there are potential differences in metabolic capabilities and phenotypic traits among microbiomes in different microhabitats.DiscussionIn conclusion, this study demonstrated the crucial role of the microenvironment in influencing the cotton microbiome and offered insights into the structures and functions of the cotton seed microbiome, facilitating future crop yield enhancement through core seed microbiome regulation.
Residual effects of combined vibratory and plantar stimulation while seated influences plantar pressure and spatiotemporal gait measures in individuals with Parkinson’s disease exhibiting freezing of gait
Authors: Phuenpathom Warongporn , Panyakaew Pattamon , Vateekul Peerapon , Surangsrirat Decho , Bhidayasiri Roongroj 
Published: January 2024

IntroductionCombined plantar pressure and vibratory stimulation has been shown to decrease freezing of gait (FOG) episodes and improve spatiotemporal gait parameters compared to single stimulation in Parkinson’s disease (PD) patients with FOG. However, the effect of combined plantar stimulations on plantar pressure analysis has never been explored.MethodsForty PD patients with frequent FOG were allocated to either FOG shoes embedded with a 100 Hz vibratory stimulation at the Achilles tendons and a soft thickened silicone pad at the hallux and sole, or sham shoes with a non-working vibratory motor and a flat non-pressure silicone pad (20 patients per arm) while seated for 96 s. The objective gait and plantar pressure analysis were measured immediately after the stimulation. Outcomes included the normalized percentage of changes in percent FOG (%FOG) and plantar pressure in the heel-strike and push-off phase that were compared between pre- and post-stimulations.ResultsThe FOG shoes group showed significantly decreased %FOG (81.5 ± 28.9% vs. 6.8 ± 22.1%, p &lt; 0.001), plantar pressure in the heel-strike (47.8 ± 43.7% vs. 4.3 ± 9.8%, p &lt; 0.001), plantar pressure in the push-off (57.7 ± 59.6% vs. 6.2 ± 11.6%, p &lt; 0.001), force time integral (FTI) (40.9 ± 32.5% vs. 6.6 ± 17.3%, p &lt; 0.001), and decreased heel contact time (19.3 ± 12.3% vs. 22.7 ± 32.5%, p &lt; 0.001) when compared to the sham group. There was a strong negative correlation between %FOG and peak plantar pressure (r = −0.440, p = 0.005), plantar pressure in the heel-strike (r = −0.847, p &lt; 0.001).ConclusionOur study demonstrated that the FOG shoe could decrease FOG episodes by improving the heel-strike pressure, toe push-off and normalized heel-to-toe plantar pressure, suggesting that modification inputs from the peripheral sensory systems might significant improvement in FOG in PD.
Domingo Sánchez y Sánchez (1860–1947): Cajal’s man on the nervous system of invertebrates
Authors: Serrano-Herrera Adela , Espinosa-Sanchez Juan Manuel 
Published: January 2024

Domingo Sánchez y Sánchez (1860–1947), a distinguished disciple of Santiago Ramón y Cajal, played a fundamental role in the Spanish School of Neurohistology through the meticulous use of diverse staining and microscopic techniques in the study of the histology and physiology of the invertebrate nervous system, generating valuable contributions that were recognized and cited by the scientific community. His research covered a wide range of areas: he was initially an anthropologist and zoologist, later earning a doctorate in Medicine and specializing in the neurohistology of invertebrates, including the detailed study of the retina and nerve centers of insects, and the discovery of histolysis in nerve centers of insect larvae during metamorphosis, challenging scientific paradigms of the time. Furthermore, Sánchez’s work on the neurofibrils of insects was crucial in supporting Cajal’s neuronal theory and refuting Bethe and Apathy’s reticularist hypothesis. Additionally, he also made preliminary observations of the Golgi apparatus, the lysosomal system, the endoplasmic reticulum, and the sarcoplasmic reticulum of skeletal muscles (Cajal-Fusari network). Domingo Sánchez y Sánchez’s exceptional scientific research and contributions to neurohistology in 20th century Spain continue to serve as a significant legacy.Life science identifiersApis mellifera: urn:lsid:zoobank.org:act:9082C709-6347-4768-A0DC-27DC44400CB2Helix aspersa: urn:lsid:zoobank.org:act:9099927E-24DF-4F89-B352-6B7902CD4A38
Argon cold atmospheric plasma eradicates pathogens in vitro that are commonly associated with canine bacterial keratitis
Authors: Marx Anne Helene , Oltmanns Hilke , Meißner Jessica , Verspohl Jutta , Fuchsluger Thomas , Busse Claudia 
Published: January 2024

PurposeTo investigate the antimicrobial effect of cold atmospheric plasma (CAP) on pathogens associated with canine bacterial keratitis.Materials and methodsPseudomonas aeruginosa, Staphylococcus pseudintermedius, and Streptococcus canis strains, which were obtained from dogs with infectious keratitis, were subjected to testing. For each species, four isolates and a reference strain were cultivated on Columbia sheep blood agar and treated with the kiNPen Vet® plasma pen from Neoplas GmbH, Greifswald, Germany. Various continuous treatment durations (0.5, 2, and 5 min) were applied, along with a 0.5-min treatment repeated four times at short intervals. These treatments were conducted at distances of 3 and 18 mm between the agar surface and the pen.ResultsCAP treatment reduced bacterial growth in all three species. The most effective treatment duration was 5 min at 3 mm distance, resulting in inhibition zones ranging from 19 to 22 mm for P. aeruginosa, 26–45 mm for S. pseudintermedius and an overall reduction of bacterial growth for Str. canis. Inhibition zones were smaller with decreasing treatment duration and larger distance. Treatment times of 30 s repeated four times and 2 min showed comparable results. Treatment with argon alone did not lead to visible reduction of bacterial growth.ConclusionArgon cold atmospheric plasma demonstrated a potent in vitro antimicrobial effect on P. aeruginosa, S. pseudintermedius and Str. canis strains with the latter showing the highest sensitivity.
La recherche d'un diplôme ou d'un mieux être social ?
Authors: Durlach Marie-Thérèse 
Published: January 2024

Nous avons essayé d'appréhender la demande en formation des femmes immigrées à travers des comptes rendus d'interviews et des témoignages, mais l'avons-nous vraiment saisie ? Derrière la demande explicite : «Je veux apprendre ceci ou cela», un autre désir, difficile à formuler pour soi et pour les autres, ne se profile-t-il pas ?
Marie-Thérèse DURLACH nous aide à aborder ce problème complexe.
Mars 1990. Questions de vocabulaire : ethnocentrisme, xénophobie
Authors: Simon Pierre-Jean , Rudder Véronique de 
Published: January 2024

Une équipe de chercheurs travaille actuellement à la rédaction d’un Vocabulaire historique et critique des relations interethniques, ces dernières étant définies par les auteurs comme couvrant « les rapports entre groupes et communautés à l’intérieur de la société nationale, et les représentations et relations entre peuples ». Ce volumineux ouvrage, dont la réalisation est prévue sur trois ans, est destiné à tous ceux qui ont à traiter des relations interethniques : étudiants, journalistes, travailleurs sociaux, etc. Il s’agit de présenter un inventaire et un examen, historique et critique, d’un vocabulaire dont les termes appartiennent à la recherche scientifique, au sens commun et à la circulation médiatique. À titre d’illustration, voici deux notices de ce vocabulaire (en version provisoire), rédigées par des membres du comité de direction du projet, dirigé par René Gallissot (la notice « Racisme » n’est pas rédigée à ce jour).
Examining the impact of a health promotion peer education programme on adolescents’ self-esteem, peer relationship culture and motivation
Authors: Simon Nóra , Csóka Angelika , Nagy Bianka Boglárka , Pente Petra , Gyulai Anikó 
Published: January 2024

Fostering positive self-esteem is key to promoting healthy behaviour in adolescents. Adolescents need to be taught to recognise negative inner voices, challenge them and replace them with positive reinforcements. Providing opportunities for peer and emotional support is also key to promoting positive self-esteem. We plan to develop a peer educator health promotion programme to create a viable peer educator model, supported by measures of effectiveness. The proposed peer educator health promotion model programme is called 'The Way to Go' and will target a group of high school youth. The peer educators are health science students currently studying for a degree in health visitors. The programme is based on three interrelated themes: self-awareness, peer relationships and motivating life goals. Our study presents the literature that supports the rationale for the proposed programme.
Hubungan Tingkat Stress dan Tingkat Pengetahuan Orang Tua terhadap Kejadian Kekerasan pada Anak Usia Sekolah di Kota Samarinda
Authors: Wulandari Reni Tri , Zulaikha Fatma 
Published: January 2024

Latar Belakang : Kekerasan pada anak dapat disebebkan oleh berbagai faktor diantaranya adalah stress dan pengetahuan orang tua, Stress dapat meningkatkan resiko kekerasan pada anak diantaranya adalah pengangguran dan penyakit, sementara pengetahuan orang tua dalam membina keluarga dan mendidik anak juga berpengaruh terhadap kekerasan pada anak. Tujuan dari penelitian ini adalah menganalisis hubungan tingkat stress dan tingkat pengetahuan orang tua terhadap kekerasan pada anak usia sekolah di kota samarinda.
Metode : Penelitian ini menggunakan metode kuantitatif korelasional dengan pendekatan cross sectional, teknik pengambilan sampel adalah stratified random sampling dengan jumlah sampel 182 responden dan pengambilan data dilakukan dengan menggunakan kuesioner. Analisis bivariat menggunakan Spearman Rank.
Hasil : Sebagian besar responden berusia 35 – 44 berjumlah 82 orang (44,8%), perempuan 124 orang (68,1%), pendidikan SLTA/sederajat 103 orang (56,6%), ibu rumah tangga 88 orang (48,4%), pendapatan <3.201.396,04 (UMR) sebanyak 92 orang (50,5%). Hasil analisis spearman rank didapatkan ada hubungan yang sangat lemah antara tingkat stress orang tua dengan kejadian kekerasan pada anak (r = -0,112) dan menunjukkan arah yang negatif, ada hubungan yang sangat lemah antara tingkat pengetahuan orang tua terhadap kejadian kekerasan pada anak (r = 0,006) dan menunjukkan arah yang positif.
Kesimpulan : Tidak ada hubungan yang signifikan antara tingkat stress orang tua terhadap kejadian kekerasan pada anak, tidak ada hubungan yang signifikan antara tingkat pengetahuan orang tua terhadap kejadian kekerasan pada anak.
Kata kunci : Kekerasan, Pengetahuan, Stress
Identifikasi Bakteri pada Luka Ulkus Pasien Diabetes Mellitus
Authors: Mita Zuliana Nur , Suliati Suliati , Endarini Lully Hanny 
Published: January 2024

Diabetes melitus adalah gangguan metabolisme yang terkait dengan sekresi insulin yang ditandai dengan hiperglikemia dan metabolisme protein, karbohidrat, dan lemak yang tidak teratur. Salah satu komplikasi diabetes melitus adalah masalah kaki diabetik mengakibatkan luka terbuka di permukaan kulit. Tujuan penelitian ini adalah untuk mengetahui hasil identifikasi bakteri yang terdapat pada luka ulkus pasien diabetes melitus di Rumah Luka Sidoarjo. Penelitian bersifat deskriptif yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya pada bulan April hingga Mei 2023. Untuk penelitian ini, digunakan 40 sampel luka ulkus dari pasien diabetes melitus yang melakukan pembersihan luka di Rumah Luka Sidoarjo menggunakan teknik konvensional dan uji biokimia digunakan untuk mengidentifikasi koloni bakteri yang tumbuh pada media agar. Penelitian terhadap 40 sampel luka ulkus pada penderita diabetes melitus mengungkapkan prevalensi berbagai jenis bakteri, antara lain bakteri gram positif dan gram negatif. Terdapat bakteri gram positif, termasuk Staphylococcus aureus (22,5%) dan Staphylococcus epidermidis (22,5%). Proteus mirabilis (7,5%), Pseudomonas aeruginosa (7,5%), Klebsiella pneumoniae (15%), Enterobacter agglomerans (2,5%), Enterobacter aerogenes (5%), Escherichia coli (12%), dan Citrobacter freundii (5%) adalah bakteri gram negatif. Menurut temuan penelitian, mayoritas bakteri gram positif yang menyebabkan ulkus pada penderita diabetes melitus adalah Staphylococcus aureus (22%) dan Staphylococcus epidermidis (22%).
Pengaruh Lama Demam terhadap Positivitas Rate IgM Anti Salmonella typhi pada Pasien Tersangka Demam Tifoid Metode Inhibition Binding Immunoassay
Authors: Nurhayati Nuli , Rohayati Rohayati , Merdekawati Fusvita , Marliana Nina 
Published: January 2024

Latar Belakang: Demam tifoid merupakan suatu penyakit infeksi sistemik yang disebabkan oleh bakteri Salmonella typhi. Manifestasi klinis demam tifoid yang pasti adalah demam. Demam terjadi pada saat tubuh sedang berusaha untuk melawan infeksi patogen dengan aktivasi sistem imun. Respon imun yang khas dimulai dengan peningkatan antibodi IgM terhadap antigen yang menstimulasi (imunogen). IgM mulai terbentuk pada hari ke-3, dan titernya meningkat hingga mencapai puncaknya pada hari ke-14. Pemeriksaan IgM anti Salmonella menggunakan metode IMBI karena memiliki tes spesifisitas dan sensitifitas yang tinggi. Tujuan penelitian ini untuk mengetahui pengaruh lama demam pada pasien demam tifoid terhadap positivitas rate IgM anti Salmonella.
Metode: Penelitian dilakukan di Rumah Sakit Kabupaten Sumedang dan Rumah Sakit Kota Bandung, dengan jenis penelitian observasional analitik cross sectional. Pada periode Mei 2023 sampel yang diambil adalah 32 pasien demam tifoid dengan kadar IgM yang diperiksa menggunakan metode IMBI. Pengambilan sampel dilakukan secara purposive sampling dan diolah dengan menggunakan Microsoft Excel.
Hasil: Data yang diperoleh dari hasil perhitungan yaitu positivitas rate IgM anti Salmonella typhi pada lama demam 2 hari adalah 0%; 4 hari adalah 25%; 6 hari adalah 75%; 8 hari adalah 50%.
Kesimpulan: Hasil penelitian ini menunjukan bahwa lama demam hari ke 2, 4, 6, dan 8 berpengaruh secara terhadap positivitas rate IgM anti Salmonella typhi.