bluuebunny/crossref_metadata_2025_split huggingface dataset:
An immunohistochemical atlas of necroptotic pathway expression
Authors: Chiou Shene , Al-Ani Aysha H. , Pan Yi , Patel Komal M. , Kong Isabella Y. , Whitehead Lachlan W. , Light Amanda , Young Samuel N. , Barrios Marilou , Sargeant Callum , Rajasekhar Pradeep , Zhu Leah , Hempel Anne , Lin Ann , Rickard James A. , Hall Cathrine , Gangatirkar Pradnya , Yip Raymond K.H. , Cawthorne Wayne , Jacobsen Annette V. , Horne Christopher R. , Martin Katherine R. , Ioannidis Lisa J. , Hansen Diana S. , Day Jessica , Wicks Ian P. , Law Charity , Ritchie Matthew E. , Bowden Rory , Hildebrand Joanne M. , O’Reilly Lorraine A. , Silke John , Giulino-Roth Lisa , Tsui Ellen , Rogers Kelly L. , Hawkins Edwin D. , Christensen Britt , Murphy James M. , Samson André L. 
Published: November 2023

AbstractNecroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell deathin vivohas proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis regulators – Caspase-8, RIPK1, RIPK3 and MLKL – in formalin-fixed mouse and human tissues. We observed surprising heterogeneity in protein expression within tissues, whereby short-lived immune barrier cells were replete with necroptotic effectors, whereas long-lived cells lacked RIPK3 or MLKL expression. Local changes in the expression of necroptotic effectors occurred in response to insults such as inflammation, dysbiosis or immune challenge, consistent with necroptosis being dysregulated in disease contexts. These methods will facilitate the precise localisation and evaluation of necroptotic signalingin vivo.Highlights13 automated immunohistochemistry protocols for detecting the necroptotic pathwayNecroptotic pathway expression is confined to fast-cycling immune barriersNecroptotic pathway expression changes at sites of immunoinflammatory challengeImmunodetection of necrosomes in IBD patients is a putative new diagnostic tool
The curse of zombie dispersal in discrete-time models of spatial population dynamics
Authors: Neubert Michael G. , van Daalen Silke F. 
Published: November 2023

AbstractIn many metapopulation and metacommunity models, individuals disperse between discrete habitat patches. When those models treat time as a discrete variable, the formulation of the dispersal term must be handled with care. A commonly made mistake is to model dispersal with terms identical to those found in continuous-time models. Such terms can inadvertently resurrect dead individuals, effectively creating “zombie dispersers.” Zombie dispersal, in turn, can have dramatic, but spurious, effects on model dynamics. In this manuscript, we illustrate the misleading effects generated by zombie dispersal in a published model used to investigate how dispersal mediates synchrony in population dynamics.
Safety and immunogenicity of COReNAPCIN®, a SARS-CoV-2 mRNA vaccine; a randomized, double-blind, placebo-controlled phase 1 clinical trial
Authors: Salehi Mohammadreza , Darazam Ilad Alavi , Nematollahi Alireza , Alimohammadi Masoumeh , Pouya Sedigheh , Alimohammadi Reza , Khajavirad Nasim , Porgoo Meysam , Sedghi Mosslim , Sepahi Mohammad Mahdi , Azimi Maryam , Hosseini Hamed , Hashemi Seyed Mahmoud , Dehghanizadeh Somaye , Khoddami Vahid 
Published: November 2023

The repeated COVID-19 outbreaks, despite global vaccination, highlights the need for booster doses. Here, we present the outcomes, up until day 90 post vaccination, of a randomized, double-blind, placebo-controlled phase 1 clinical trial of the mRNA-based vaccine candidate; COReNAPCIN®, as a booster dose in adults aged 18-50 who had previously received three doses of inactivated vaccines. In the study, 30 participants randomly (2:2:1) received 25 μg, or 50 μg of COReNAPCIN®, or placebo. The results indicated that COReNAPCIN®was well tolerated in vaccinated individuals in both groups with no life-threatening or other serious adverse events. The most noticeable solicited adverse events were pain at the site of injection, fatigue and myalgia. Regarding the immunogenicity, given the seroprevalence of SARS-CoV-2 antibodies due to the vaccination history for all, and previous SARS-CoV-2 infection for some participants, the recipients of COReNAPCIN®, two weeks post vaccination, showed significant fold increases in the level of anti-RBD (6.6 and 8.1 folds) or anti-spike (11.5 and 21.7 folds), and potent neutralizing antibodies (10.2 and 8.4 folds) in 25 and 50 μg groups, respectively, while no meaningful changes were observed in the placebo group. Additionally, the significant increase in the spike-specific IFN-γ T-cell response upon vaccination, underscores the activation of cellular immunity. Altogether, the favorable safety, tolerability, and immunogenicity profile of COReNAPCIN®support its further clinical development.Trial registration numberIRCT20230131057293N1
Single-molecule analysis of purified proteins and nuclear extracts: insights from 8-oxoguanine glycosylase 1
Authors: Schaich Matthew A. , Weaver Tyler M. , Roginskaya Vera , Freudenthal Bret D. , Van Houten Bennett 
Published: November 2023

AbstractBy observing one molecule at a time, single-molecule studies can offer detailed insights about biomolecular processes including on rates, off rates, and diffusivity of molecules on strands of DNA. A recent technological advance (Single-molecule Analysis of DNA-binding proteins from Nuclear Extracts, SMADNE) has lowered the barrier to entry for single-molecule studies, and single-molecule dynamics can now be determined directly out of nuclear extracts, providing information in an intermediate environment between purified proteins in isolation and the heterogeneity of a nucleus. To compare and contrast the single-molecule DNA binding dynamics in nuclear extracts versus purified proteins, combined optical tweezers and fluorescence microscopy experiments were performed with purified GFP-tagged 8-oxoguanine glycosylase 1 (OGG1), purified GFP-OGG1 spiked into nuclear extracts, and nuclear extracts from human cells overexpressing GFP-OGG1. We observed differences in undamaged DNA binding during DNA damage search in each of the three conditions. Purified GFP-OGG1 engaged undamaged DNA for a weighted average lifetime of 5.7 s and 21% of these events underwent DNA diffusion after binding. However, unlike other glycosylases studied by SMADNE, OGG1 does not bind non-damaged DNA efficiently in nuclear extracts. In contrast, GFP-OGG1 binding dynamics on DNA substrates containing oxidative damage were relatively similar in all three conditions, with the weighted average binding lifetimes varying from 2.2 s in nuclear extracts to 7.8 s with purified GFP-OGG1 in isolation. Finally, we compared the purified protein and nuclear extract approaches for a catalytically dead OGG1 variant (GFP-OGG1-K249Q). This variant greatly increased the binding lifetime for oxidative DNA damage, with the weighted average lifetime for GFP-OGG1-249Q in nuclear extracts at 15.4 s vs 10.7 s for the purified protein. SMADNE will provide a new window of observation into the behavior of nucleic acid binding proteins only accessible by biophysicists trained in protein purification and protein labeling.
Adapting to climate change: substitution effect of water on residential electricity consumption
Authors: Zhang Fan , Cao Ming 
Published: November 2023

Purpose
As climate change impacts residential life, people typically use heating or cooling appliances to deal with varying outside temperatures, bringing extra electricity demand and living costs. Water is more cost-effective than electricity and could provide the same body utility, which may be an alternative choice to smooth electricity consumption fluctuation and provide living cost incentives. Therefore, this study aims to identify the substitute effect of water on the relationship between climate change and residential electricity consumption.

Design/methodology/approach
This study identifies the substitute effect of water and potential heterogeneity using panel data from 295 cities in China over the period 2004–2019. The quantile regression and the partially linear functional coefficient model in this study could reduce the risks of model misspecification and enable detailed identification of the substitution mechanism, which is in line with reality and precisely determines the heterogeneity at different consumption levels.

Findings
The results indicate that residential water consumption can weaken the impact of cooling demand on residential electricity consumption, especially in low-income regions. Moreover, residents exhibited adaptive asymmetric behaviors. As the electricity consumption level increased, the substitute effects gradually get strong. The substitute effects gradually strengthened when residential water consumption per capita exceeds 16.44 tons as the meeting of the basic life guarantee.

Originality/value
This study identifies the substitution role of water and heterogeneous behaviors in the residential sector in China. These findings augment the existing literature and could aid policymakers, investors and residents regarding climate issues, risk management and budget management.
Design and analysis of combined standard ferrofluid and centrifugal seals
Authors: Szczęch Marcin , Raj Kuldip 
Published: November 2023

Purpose
Ferrofluid seals are known for their low friction torque and high tightness. However, they have some limitation due to the allowable rotational speed. The work presented here analyzes the performance of newly designed seals which are a combination of a ferrofluid and a centrifugal seal. The new seals can operate at high speeds. The purpose of this study is to theoretically predict the performance of combined seals.

Design/methodology/approach
Three seals were designed and selected for analysis. A version of the seals with a nonmagnetic insert is also considered, the purpose of which is to facilitate the installation and return of ferrofluid during low rotational speeds. The analyses were based on combining the results of numerical simulation of magnetic field distribution with mathematical models.

Findings
A combination of ferrofluid sealing and centrifugal sealing is possible. Analyses showed that the combined seal could hold a minimum pressure of 190 kPa in the velocity range of 0–100 m/s. The problem with this type of seal is the temperature.

Originality/value
New seal designs are presented. Key parameters that affect the seal operation are discussed. A methodology that can be used in the design of such seals is presented.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-07-2023-0221/.
Behavioural finance: the decoy effect on stock investment decisions
Authors: Okumura Bruno Uekane , Pimenta Júnior Tabajara , Maemura Márcia Mitie Durante , Gaio Luiz Eduardo , Gatsios Rafael Confetti 
Published: November 2023

PurposeThis study aims to investigate the occurrence of the decoy effect in stock investment decisions based on fundamental analysis.Design/methodology/approachIn this study, the decoy effect was investigated by applying two questionnaires, one of them with the presence of a decoy alternative, to a set of 224 respondents with knowledge of business fundamentals, simulating investment decisions in stocks of companies listed on the Brazilian Stock Exchange. The data analysis was performed using the Fisher's exact test, Student's t-test and ANOVA. The research also aimed to detect a potential relationship between the variables gender, age, degree and professional experience with the type of decision made.FindingsThe results pointed to the occurrence of the decoy effect when analysing the general response data. However, such evidence was not confirmed when the sample was analysed by classes (gender, course, age and professional experience). There is no statistical evidence that the decoy effect influences classes.Originality/valueThe recent decoy effect literature is little explored in investment decision-making. This study is unique in examining the decoy effect in investment decisions in the Brazilian context.
Economic integration and stock market linkages: evidence from South Africa and BRIC
Authors: Gopane Thabo J. 
Published: November 2023

PurposeThis study examines the impact of regional economic integration (REI) on stock market linkages in the BRICS (Brazil, Russia, India, China and South Africa) economic bloc. In this type of study, the BRICS framework is an appealing empirical case, given its uncommon characteristics. For example, BRICS member states come from remote geographic locations (Africa, Asia, Europe and South America) and have contrasting socioeconomic profiles.Design/methodology/approachAn empirical design is framed from the perspective of bilateral trade between South Africa and BRIC. The author accepts trade intensity as a proxy of regional economic integration and then examines the resulting effect on the stock market co-movement within BRIC. The study applies a two-step econometric procedure of the BEKK-MGARCH and panel data models.FindingsOverall, bilateral trade, as a proxy of economic inwctegration, is associated with an increase in stock market integration. This positive relationship is particularly observed during episodes of surplus trade, and more interestingly, was initiated three years after BRICS’ existence and continues to grow at an increasing rate.Practical implicationsThe study outcome should benefit international trade practitioners and global investors interested in portfolio diversification or concerned with risk spillovers.Originality/valueFirst, notwithstanding South Africa's significant economic presence in the African continent, to the best of the author’s knowledge, this is the first study to empirically evaluate the BRICS economic integration on their stock market linkages from the perspective of South Africa. The value of this contribution is that further work may investigate the bidirectional spillover impact conveyed by South Africa's trade interactions within the juxtaposition of Africa and BRICS economies. Second, given that research on REI and stock market integration has historically concentrated on mature regional blocs of Europe, Asia, South and North America, the current study advances knowledge while correcting the prevailing literature imbalance.
Community support and benefits, culture and hedonism as moderators in the relationship between brand heritage, tourist satisfaction and revisit intention
Authors: Raja Sathish , Soundararajan Vasanthi , Parayitam Satyanarayana 
Published: November 2023

PurposeThis research explores the antecedents of tourist satisfaction and revisit intention, especially in light of the post-pandemic environment that pushed tourism to the lowest level in the present decade. A comprehensive conceptual model based on the brand equity theory (BET) is developed, and interrelationships between brand heritage, community support and benefits, hedonism, culture, tourist satisfaction and revisit intention.Design/methodology/approachThe data were collected from 670 tourists visiting one of the heritage destinations in southern India (Kanchipuram). After checking the psychometric properties of the survey instrument, the hypotheses were tested using path analysis and Hayes's PROCESS macros.FindingsThe results indicate that heritage brand of destination sites is positively associated with (1) tourist satisfaction and (2) tourist revisit intention. The findings also support the positive effect of attractiveness of products is positively related to tourist satisfaction, which in turn, is a significant predictor of tourist revisit intention. The findings also support that community support benefits moderating the relationship between heritage brand and attractiveness of products in heritage destinations tourist satisfaction.Practical implicationsThis study has several implications for practicing managers engaged in maintaining heritage destinations. As this research highlights the importance of heritage brands of destinations in influencing tourist satisfaction, local communities and policymakers need to provide the necessary infrastructure and facilities at these destinations. Further, local entrepreneurs are motivated to invest in delivering products that attract tourists.Originality/valueThe conceptual model developed in this study, is first of its kind, to the best of the authors’ knowledge, that investigates the effect of hedonism (second moderator) moderating the relationship between tourist satisfaction and culture (first moderator) in influencing tourists to revisit their intention. In addition to direct relationships, the moderating role of community support and benefits, culture and hedonism makes a unique contribution to the bourgeoning literature on tourism especially in Indian context.
Food security and trade: public stockholding through the lens of economies and law
Authors: Bhattacharya Krishna , Ahuja Mahima 
Published: November 2023

Purpose
The debate to find a solution for domestic price support under the WTO Agreement on Agriculture (AOA) has been a long one. The stance of India is critical to determine due to its large population. This paper aims to analyse the benefits or demerits of minimum price support and what approach could be adopted by India.

Design/methodology/approach
The paper is a mix of both analytical and theoretical research. The paper first provides a background on the issues related to public stockholding and further analyses some data at which India procures wheat and rice from the farmers and then compares it with retail market prices in India.

Findings
The paper finds that the difference in price between minimum price support and retail market prices in India for wheat and rice is minimal. Therefore, the concern that India might be taking advantage of the minimum price is uncalled for. India also needs to balance its own interests as well as abide by its WTO obligations. The paper finds that cooperation among countries or regional blocks might help to address the problem of food insecurity.

Originality/value
The paper portrays India’s stance with regard to WTO AOA as well as studies the Indian market for wheat and rice.
A dynamic threshold analysis of effect of Financial Action Task Force (FATF) measures on financial inclusion: evidence from the world
Authors: Urooj Shama 
Published: November 2023

Purpose
This study aims to examine the effect of Financial Action Task Force (FATF) compliance on the degree of financial inclusion (FI) across 174 economies during the period from 2011 to 2021, including developed and developing countries.

Design/methodology/approach
This paper uses panel dynamic threshold regression to examine whether there is a threshold effect that exists in FATF compliance.

Findings
The findings show that FATF regulations enhance financial inclusiveness all over the world, but at the same time, FATF regulations regarding AML/CFT implications impose a high cost on financial institutions above the threshold of FATF compliance.

Research limitations/implications
This study’s findings indicate that nations should undertake deliberate struggle to reduce the prevalence of money laundering (ML) and terrorism financing by putting in place effective FATF regulatory frameworks to support FI.

Originality/value
This study’s findings indicate that nations should undertake deliberate struggle to reduce the prevalence of ML and terrorism financing by putting in place effective FATF regulatory frameworks to support FI. Regulators must, however, guarantee that the process is cost-effective and efficient.
Complex interactions between diverse mobile genetic elements drive the evolution of metal‐resistant bacterial genomes
Authors: Mahbub Khandaker Rayhan , Chénard Caroline , Batinovic Steven , Petrovski Steve , Lauro Federico M. , Rahman Md Hafizur , Megharaj Mallavarapu , Franks Ashley E. , Labbate Maurizio 
Published: November 2023

AbstractIn this study, we compared the genomes of three metal‐resistant bacteria isolated from mercury‐contaminated soil. We identified diverse and novel MGEs with evidence of multiple LGT events shaping their genomic structure and heavy metal resistance. Among the three metal‐resistant strains, Sphingobium sp SA2 and Sphingopyxis sp SE2 were resistant to multiple metals including mercury, cadmium, copper, zinc and lead. Pseudoxanthomonas sp SE1 showed resistance to mercury only. Whole genome sequencing by Illumina and Oxford Nanopore technologies was undertaken to obtain comprehensive genomic data. The Sphingobium and Sphingopyxis strains contained multiple chromosomes and plasmids, whereas the Pseudoxanthomonas strain contained one circular chromosome. Consistent with their metal resistance profiles, the strains of Sphingobium and Sphingopyxis contained a higher quantity of diverse metal resistance genes across their chromosomes and plasmids compared to the single‐metal resistant Pseudoxanthomonas SE1. In all three strains, metal resistance genes were principally associated with various novel MGEs including genomic islands (GIs), integrative conjugative elements (ICEs), transposons, insertion sequences (IS), recombinase in trio (RIT) elements and group II introns, indicating their importance in facilitating metal resistance adaptation in a contaminated environment. In the Pseudoxanthomonas strain, metal resistance regions were largely situated on a GI. The chromosomes of the strains of Sphingobium and Sphingopyxis contained multiple metal resistance regions, which were likely acquired by several GIs, ICEs, numerous IS elements, several Tn3 family transposons and RIT elements. Two of the plasmids of Sphingobium were impacted by Tn3 family transposons and ISs likely integrating metal resistance genes. The two plasmids of Sphingopyxis harboured transposons, IS elements, an RIT element and a group II intron. This study provides a comprehensive annotation of complex genomic regions of metal resistance associated with novel MGEs. It highlights the critical importance of LGT in the evolution of metal resistance of bacteria in contaminated environments.
Spatial growth and convergence in Indian agriculture
Authors: Balaji Sedithippa J. , Gopinath Munisamy 
Published: November 2023

AbstractThe economic growth convergence between advanced nations and the rest of the world has been found to reduce global income inequality. However, less is known about convergence within nations, especially in developing economies with substantial regional heterogeneities. Using the neoclassical framework, this study tests for farm income convergence in India, where agriculture employs over 200 million workers. Household farm income is estimated in 599 districts using three pan‐India situation assessment surveys (2003, 2013, and 2019). The conditional convergence model—augmented to capture spatial spillovers—shows farm incomes are converging across Indian districts but at slower rates in recent years and among farmers in the left‐tail of the income distribution. Irrigation infrastructure, crop diversification, and distance to the urban market explain farm income growth, and hence, spatial income disparities. Returns to these factors are high for middle‐income farmers than for the rich or poor. Spatial spillovers, likely from technological change, captured in residuals also had a positive impact on farm incomes. Results remain unchanged when farm income was replaced by its value added. Connecting the poorest to the markets, strengthening infrastructure and promoting diversification for the middle‐income groups, and nudging high‐income farmers toward value chains could help equitable economic development.