bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theranostic Near-Infrared Monoamine Oxidase Inhibitor (NMI) Protein Binding Interactions with MAOA and Albumin
Authors: Irwin Ronald W. , Shah Unnati H. , Soni Shivani , Lenz Heinz Josef , Shih Jean C. 
Published: February 2025

Abstract

Purpose
The protein binding interactions of near-infrared monoamine oxidase inhibitor (NMI) are reported here.

Methods
NMI-bound proteins were examined by fluorescent SDS-PAGE and mass spectrometry using tumor tissues from brain and colon cancer mouse models.

Results
This study shows protein interactions with NMI, a chemical conjugate of MAOA inhibitor clorgyline and tumor-seeking dye, MHI-148. NMI fluorescence in MAOA knock-out (KO) mice was significantly lower compared to WT mice, including whole animal, organs, and tissue lysates which indicated that NMI binds to MAOA. Pure recombinant MAOA protein was detectable as a single fluorescent band that migrated at ~ 65kD. NMI inhibited MAOA activity (IC50 1–5 µM). In a glioma mouse model, NMI targeted specifically to tumor with high contrast to adjacent normal brain, shown by a 65 kD protein band. Recent studies demonstrated heptamethine cyanine dyes (e.g., MHI-148) interact with serum albumin, contributing to tumor uptake and cancer cell internalization. Our study shows NMI binds to albumin but highly prefers MAOA, providing a plausible mechanism for systemic drug delivery via serum albumin to the tumor target and subsequent MAOA inhibition. Further studies in a colon cancer mouse model found the ~ 65 kD SDS-PAGE band, bound to NMI, contained both MAOA and albumin proteins by mass spectrometry.

Conclusion
NMI was shown to interact with MAOA and the blood carrier protein, albumin. This study provides insights for drug delivery and protein target specificity of NMI to image and treat cancer.
Low-Temperature Aqueous Alteration of Chondrites
Authors: Lee Martin R. , Alexander Conel M. O’D. , Bischoff Addi , Brearley Adrian J. , Dobrică Elena , Fujiya Wataru , Le Guillou Corentin , King Ashley J. , van Kooten Elishevah , Krot Alexander N. , Leitner Jan , Marrocchi Yves , Patzek Markus , Petaev Michail I. , Piani Laurette , Pravdivtseva Olga , Remusat Laurent , Telus Myriam , Tsuchiyama Akira , Vacher Lionel G. 
Published: February 2025

Abstract
Chondritic meteorites (chondrites) contain evidence for the interaction of liquid water with the interiors of small bodies early in Solar System history. Here we review the processes, products and timings of the low-temperature aqueous alteration reactions in CR, CM, CI and ungrouped carbonaceous chondrites, the asteroids Ryugu and Bennu, and hydrated dark clasts in different types of meteorites. We first consider the nature of chondritic lithologies and the insights that they provide into alteration conditions, subdivided by the mineralogy and petrology of hydrated chondrites, the mineralogy of hydrated dark clasts, the effects of alteration on presolar grains, and the evolution of organic matter. We then describe the properties of the aqueous fluids and how they reacted with accreted material as revealed by physicochemical modelling and hydrothermal experiments, the analysis of fluid inclusions in aqueously formed minerals, and isotope tracers. Lastly, we outline the chronology of aqueous alteration reactions as determined using the 53Mn-53Cr and 129I-129Xe systems.
Mesoporous Silica-Polyethyleneimine Composites as High-Capacity Adsorbents for CO2 Adsorption: Isotherm and Thermodynamic Analysis
Authors: Yargıç Adife Şeyda , Şener Mustafa 
Published: February 2025

Abstract
In this study, polyethyleneimine-mesoporous silica composite materials were prepared and the effectiveness of the promising sorbents in adsorbing CO2 was evaluated, along with the impacts of the silica support types (Mesoporous Silica Nanoparticles (MSN) and Mobil Composition of Matter No.48 (MCM-48)), polyethyleneimine (PEI) loading percentages (50 and 70 wt.%), calcination, surface functionalization by alkyl chains (CTMABr), and adsorption temperature (75 and 100 °C). The analysis’s results revealed that the pores of the sorbents were mostly covered with PEI molecules following PEI-functionalization, and the specific surface area and pore volume were also reduced with rising amine content. The highest CO2 adsorption capacities were achieved for UC-MCM-48–50 and UC-MSN–50 at 2.26 mmol/g and 3.31 mmol/g, respectively. The CO2 uptake capacities of CC-MSN–50 and CC-MCM-48–50, composed by dispersing CTMABr surfactant with the calcined materials before incorporating PEI, were remarkably similar to those of non-surfactant functionalized adsorbents. When the temperature’s influence on CO2 adsorption capacity was evaluated, the maximum holding capability adsorbent UC-MSN–50 had a slight increase in adsorption capacity (~ 3.6%), whereas UC-MCM-48–50 had a considerable drop (~ 23.9%) as the temperature elevated to 100 °C. Besides, Langmuir, Freundlich, Dubinin-Radushkevich, and Temkin isotherms were used to model pure CO2 adsorption data, and a thermodynamic study was applied. In conclusion, a low-cost and more beneficial approach, which included less PEI handling and eliminating the calcination step, was implemented to enhance the CO2 sorption capacity of composites of PEI with the long alkyl chain template MCM-48 or MSN silica support materials.
Lung cell toxicological effects of 3D printer aerosolized filament byproducts
Authors: Beard Jonathan M. , Royer Brooke M. , Hesita Jacob M. , Byrley Peter , Lewis Ashley , Hadynski John , Matheson Joanna , Al-Abed Souhail R. , Sayes Christie M. 
Published: February 2025

Abstract
As 3D printing has become more compact and affordable, the use of the technology has become more prevalent across household, classroom, and small business settings. The emissions of fused filament fabrication (FFF) 3D printers consist of volatile organic compounds (VOCs) and aerosolized particulate matter (PM) dependent upon the filament in use. This study investigates the hazards posed by these emissions through aerosol characterization and cell exposure. Seventeen filaments were obtained from five manufacturers, consisting of fourteen plastic filaments (polylactic acid (PLA), acrylonitrile butadiene styrene (ABS), or polycarbonate (PC) polymers) and three filaments with metal filler (copper, bronze, and steel). For 1-h trials, BEAS-2B human bronchial epithelial cells were directly exposed to aerosolized 3D printer emissions at the air–liquid interface (ALI). Particle characterization showed ABS filaments produced more PM and VOC emissions with particles in the ultrafine size range. ABS filaments also elicited a greater biological response, with significant shifts in mitochondrial activity compared to the PLA filaments. Significant changes in amounts of glutathione (GSH) were observed after ABS and PLA emission exposure. Exposure to emissions from the steel filament resulted in the lowest average amount of glutathione, though insignificant, and a significantly lower mitochondrial activity, revealing a unique cause for concern among filaments tested. 3D printer emissions and subsequent cell responses appear filament-dependent, and users should mitigate personal exposure to aerosols.

Graphical abstract

Made using elements from BioRender.
Wie können Studierende über gesundheitsförderliche Angebote an ihrem Studienort informiert werden?
Authors: Kneer K. J. , Kliner A. , Kielin K. , Rüttinger D. , Walter V. , Reusch A. , Neuderth S. 
Published: February 2025

Zusammenfassung

Hintergrund
Studierende weisen spezifische gesundheitsbezogene Problemlagen auf und besitzen häufig unzureichende Kenntnisse über regionale Angebote der Gesundheitsförderung. Das Ziel der Studie war die Entwicklung und Umsetzung einer bedarfsgerechten Informationsstrategie zu gesundheitsförderlichen Angeboten.

Methode
Studierende wurden per Online-Fragebogen und in Fokusgruppen zu Bedarfen und Wünschen im Hinblick auf Gesundheitsförderung befragt. Auf Basis der Ergebnisse wurden eine Website und ein Social-Media-Kanal entwickelt und umgesetzt.

Ergebnisse
In der Befragung wurden die Gesundheitsbereiche „Stress“ und „psychische Belastung“ als besonders relevant betrachtet. In den Fokusgruppen wurde die Hochschule in der Pflicht gesehen, als Ansprechpartner zu und Anbieter von gesundheitspräventiven Leistungen aufzutreten. Über die Angebotslandschaft waren die Studierende unzureichend informiert. Die Informationssuche erfolgte digital und eher unsystematisch. Für die Website, die im November 2023 ans Netz ging, wurden die regionalen Angebote grafisch aufbereitet. Die Akzeptanz der Seite ist – basierend auf den Besucherzahlen – gut. Der Instagramkanal startete im Juni 2022 und konnte über 16.700 Konten erreichen.

Schlussfolgerung
Die Hochschulen sind gefordert, als Ansprechpartner zu und Anbieter von gesundheitsförderlichen Angeboten aufzutreten. Initiativen zum studentischen Gesundheitsmanagement (SGM) sind häufig über Projektmittel zeitlich befristet finanziert. Eine Verstetigung könnte die Gesundheitskompetenz fördern und zur Profilbildung von Hochschulen beitragen. Darüber hinaus bedarf es der zielgruppenspezifischen digitalen Information zu regionalen Angeboten und einer tragfähigen Verstetigungsstrategie.
The Influence of Different Hot-Dip Zinc Coating Types on the Gas Metal Arc Welding of Galvanized Steel Sheets
Authors: Sejč Pavol , Gábrišová Zuzana , Vanko Branislav , Belanová Judita 
Published: February 2025

Abstract
The work was focused on evaluating the influence of the structure of the protective Zn coating on steel sheets on arc welding. Two types of galvanized steel sheets were used in the experiments, HX340LAD + ZF (GA type) and HX300LAD + Z (GI type), with a thickness of 2 mm. First, the analysis of the structure of the Zn protective layer was carried out on the selected materials. After that, test welds were made using the gas metal arc (GMA) welding in the short-circuit metal transfer. The influence of the type of protective Zn coating on the occurrence of defects, the geometry of the weld, and the stability of the welding process was evaluated. From the achieved results, it can be concluded that the protective Zn layer on the HX340LAD + ZF sheet was formed by intermetallic phases FexZny-Fe concentration ranged from 19.0 wt.% (at the steel plate coating interface) up to 9.5 wt.% (on the coating surface). An Fe-Al inhibitor layer was observed at the steel sheet-coating interface of the HX300LAD + Z material, while the Fe content in the Zn coating decreased from 8.2 to 0.8 wt.% towards the surface. Better weld evaluation results were achieved on samples made on HX340LAD + ZF galvanized sheet. The welds were without porosity, and the stability parameter (electric arc stability criterion) was almost two times smaller than the stability parameter in the evaluation of the HX300LAD + Z sheet metal welding process.
Port Site Placement and Outcomes for Surgical Obesity and Metabolic Surgeries (PSPOSO) Checklist: A New Reporting Checklist Based on Evidential Assessment of the Number of Trocars and Positions
Authors: Zidan Mohamed H. , El-Masry Hassan , Amgad Ahmed , Altabbaa Hashem , Abdou Marwan Emad , Amer Samar A. , Zayed Nour , Ismail Haidy Osama , Alokl Mohammed , Abokhozima Ahmed 
Published: February 2025

Abstract
Since the early 1980s, efforts to standardize ergonomic practices in laparoscopic surgeries have aimed to improve procedural efficiency and reduce complications, but clinical validation remains limited. In metabolic and bariatric surgeries (MBS), innovations in trocar site placements, driven by the popularity of laparoscopic sleeve gastrectomy (LSG), have advanced surgical techniques. However, practices often vary based on individual surgeon preferences rather than standardized evidence-based criteria. This study introduces the Port Site Placement and Outcomes for Surgical Obesity and Metabolic Surgeries (PSPOSO) checklist, aiming to standardize port placements and improve reporting consistency. A systematic review and meta-analysis of LSG studies were conducted following PRISMA guidelines. Data were extracted from 34 studies involving 7173 cases. Key variables included port configurations, manipulation angles, and outcomes such as operative time and excess weight loss percentage (EWL%). Innovative methods were used to estimate manipulation and azimuth angles from available intraoperative images. Statistical analyses and meta-regression were performed to identify associations between port configurations and surgical outcomes. Findings revealed substantial variability in port placements, with no significant effect of manipulation angles or port numbers on operative time or EWL% at 6, 12, and 24 months (p-values > 0.05). High residual heterogeneity suggests that factors beyond manipulation angles and port counts contribute to outcome variability. The PSPOSO checklist provides a framework for standardizing port placement and ergonomic parameters in MBS, enhancing reproducibility and safety. Future studies should validate the checklist across diverse clinical settings to refine surgical approaches and improve patient outcomes. PROSPERO: CRD42024598674.
Overcoming annotation bottlenecks in underwater fish segmentation: a robust self-supervised learning approach
Authors: Saleh Alzayat , Sheaves Marcus , Jerry Dean , Azghadi Mostafa Rahimi 
Published: February 2025

Abstract
Accurate fish segmentation in underwater videos is challenging due to low visibility, variable lighting, and dynamic backgrounds, making fully-supervised methods that require manual annotation impractical for many applications. This paper introduces a novel self-supervised learning approach for fish segmentation using Deep Learning. Our model, trained without manual annotation, learns robust and generalizable representations by aligning features across augmented views and enforcing spatial-temporal consistency. We demonstrate its effectiveness on three challenging underwater video datasets: DeepFish, Seagrass, and YouTube-VOS, surpassing existing self-supervised methods and achieving segmentation accuracy comparable to fully-supervised methods without the need for costly annotations. Trained on DeepFish, our model exhibits strong generalization, achieving high segmentation accuracy on the unseen Seagrass and YouTube-VOS datasets. Furthermore, our model is computationally efficient due to its parallel processing and efficient anchor sampling technique, making it suitable for real-time applications and potential deployment on edge devices. We present quantitative results using Jaccard Index and Dice coefficient, as well as qualitative comparisons, showcasing the accuracy, robustness, and efficiency of our approach for advancing underwater video analysis.
The Metabolic Profile of Plasma During Epileptogenesis in a Rat Model of Lithium–Pilocarpine-Induced Temporal Lobe Epilepsy
Authors: Antmen Fatma Merve , Matpan Emir , Dayanc Ekin Dongel , Savas Eylem Ozge , Eken Yunus , Acar Dilan , Ak Alara , Ozefe Begum , Sakar Damla , Canozer Ufuk , Sancak Sehla Nurefsan , Ozdemir Ozkan , Sezerman Osman Ugur , Baykal Ahmet Tarık , Serteser Mustafa , Suyen Guldal 
Published: February 2025

Abstract
Temporal lobe epilepsy (TLE) arises mostly because of an initial injury. Certain stimuli can make a normal brain prone to repeated, spontaneous seizures via a process called epileptogenesis. This study examined the plasma metabolomics profile in rats with the induced TLE to identify feasible biomarkers that can distinguish progression of epileptogenesis in three different time points and reveal the underlying mechanisms of epileptogenesis. Status epilepticus (SE) was induced by repetitive intraperitoneal injections of low-dose lithium chloride–pilocarpine hydrocholoride. Blood samples were collected 48 h, 1 week, and 6 weeks after SE, respectively. Plasma metabolites were analyzed by nuclear magnetic resonance (NMR) spectrometry. Statistical analysis was performed using MetaboAnalyst 6.0. An orthogonal partial least squares discriminant analysis (OPLS-DA) model was employed to represent variations between the TLE model groups and respective controls. Volcano plot analysis was used to identify key features, applying a fold-change criterion of 1.5 and a t-test threshold of 0.05. 48 h after SE, dimethyl sulfone (DMSO2) and creatinine levels were decreased, whereas glycine and creatine levels were increased. The only metabolite that changed 1 week after SE was pyruvic acid, which was increased compared to its control level. Lactic acid, pyruvic acid, and succinic acid levels were increased 6 weeks after SE. The identified metabolites were especially related to the tricarboxylic acid cycle and glycine, serine, and threonine metabolism. The results illustrate that distinct plasma metabolites can function as phase-specific biomarkers in TLE and reveal new insights into the mechanisms underlying SE.
Successful treatment of type I cryoglobulinemia with a combination of carfilzomib, cyclophosphamide, and dexamethasone: a case report and literature review
Authors: Yasuda Yohei , Maki Hiroaki , Shimura Arika , Honda Akira , Masamoto Yosuke , Kurokawa Mineo 
Published: February 2025

Abstract
Type I cryoglobulinemia is typically associated with hematological malignancies such as B-cell lymphomas and plasma cell neoplasms. Its treatment basically targets underlying hematological malignancies, and the prognosis remains unsatisfactory. Despite several reports of type I cryoglobulinemia treated with bortezomib-based regimens, little is available on the treatment of bortezomib-resistant cases. We report a case of severe type I cryoglobulinemia associated with plasma cell neoplasm, refractory to bortezomib and daratumumab, which was successfully managed with a combination of carfilzomib, cyclophosphamide, and dexamethasone (KCd therapy). No sign of relapse has been seen for more than 3 years with maintenance therapy with ongoing carfilzomib. This case highlights the potential efficacy of carfilzomib-based regimens in bortezomib-resistant type I cryoglobulinemia, offering a promising option for cases refractory to conventional treatments.
Assessment of Geotourism Potential of the Carian Trail (SW Türkiye)
Authors: Gül Murat , Küçükuysal Ceren 
Published: February 2025

Abstract
Increasing tourism diversity and spreading it over a year is becoming prominent for local and regional economies. Among the various tourism sites in Türkiye, Muğla province has always been one of the most attractive summer tourism areas. Besides this, it also hosts numerous places of natural, historical, archeological, geological, and geomorphological values which enable year-long tourism and contribute to the sustainability and development of the regional economy. In this respect, the Carian Trail, very famous for walking over 800 km in natural beauty is selected in this study as a georoute to be promoted for its geotourism potential by its scientific, educational, cultural and ecological values. To achieve this, an inventory was created that includes geological, geomorphological, and geological heritage values, along with other key features. The inventory and characterization stage is followed by qualitative & semiquantitative approaches. As the first study on such assessments for the Carian Trail, this study highlights the need to evaluate each site individually in order to develop strategies and policies for geoconservation.
Cannabidiol-Induced Autophagy Ameliorates Tau Protein Clearance
Authors: Vrechi Talita A. M. , Guarache Gabriel C. , Oliveira Rafaela Brito , Guedes Erika da Cruz , Erustes Adolfo G. , Leão Anderson H. F. F. , Abílio Vanessa C. , Zuardi Antonio W. , Hallak Jaime Eduardo C. , Crippa José Alexandre , Bincoletto Claudia , Ureshino Rodrigo P. , Smaili Soraya S. , Pereira Gustavo J. S. 
Published: February 2025

Abstract
Tau is a neuronal protein that confers stability to microtubules; however, its hyperphosphorylation and accumulation can lead to an impairment of protein degradation pathways, such as autophagy. Autophagy is a lysosomal catabolic process responsible for degrading cytosolic components, being essential for cellular homeostasis and survival. In this context, autophagy modulation has been postulated as a possible therapeutic target for the treatment of neurodegenerative diseases. Studies point to the modulatory and neuroprotective role of the cannabinoid system in neurodegenerative models and here it was investigated the effects of cannabidiol (CBD) on autophagy in a human neuroblastoma strain (SH-SY5Y) that overexpresses the EGFP-Tau WT (Wild Type) protein in an inducible Tet-On system way. The results demonstrated that CBD (100 nM and 10 µM) decreased the expression of AT8 and total tau proteins, activating autophagy, evidenced by increased expression of light chain 3-II (LC3-II) protein and formation of autophagosomes. Furthermore, the cannabinoid compounds CBD, ACEA (CB1 agonist) and GW-405,833 (CB2 agonist) decreased the fluorescence intensity of EGFP-Tau WT; and when chloroquine, an autophagic blocker, was used, there was a reversal in the fluorescence intensity of EGFP-Tau WT with CBD (1 and 10 µM) and GW-405,833 (2 µM), demonstrating the possible participation of autophagy in these groups. Thus, it was possible to conclude that CBD induced autophagy in EGFP-Tau WT cells which increased tau degradation, showing its possible neuroprotective role. Hence, this study may contribute to a better understanding of how cannabinoids can modulate autophagy and present a potential therapeutic target in a neurodegeneration model.

Graphical Abstract
Why bumblebees have become model species in apidology: A brief history and perspectives
Authors: Lihoreau Mathieu , Monchanin Coline , Lacombrade Mathilde , Brebner Joanna , Gómez-Moracho Tamara 
Published: February 2025

Abstract
In recent years, bumblebees have increasingly been used to study various aspects of bee biology, ecology and evolution. They are now broadly accepted as tractable model species, complementary to the domestic honey bees, for fundamental and applied apidology. Here, we provide a brief history of how bumblebee research developed since their domestication and commercialisation for crop pollination in the 1990s. Bumblebees are large social bees that can be kept and trained in the lab year-round. They are easy to manipulate and track individually in their small colonies. These practical advantages have offered new possibilities for experimental bee research, leading to major breakthroughs in different fields such as cognition, navigation, nutrition, host-parasite interactions, and insect declines. Many of these findings have later been confirmed in honey bees and other pollinators. We discuss some exciting directions for future apidology research based on bumblebees.
Sexual Orientation and Gender Identity (SOGI): A Tutorial on Ethical Data Practices
Authors: Oda Fernanda S. , Stiehl Christie M. 
Published: February 2025

Abstract
In the U.S., approximately 13 million individuals identify as part of a sexual and gender minority (SGM). This broad spectrum includes sexual orientation identities such as gay, lesbian, bisexual, queer, pansexual, and same-gender-loving, as well as gender identities such as transgender, gender non-conforming, bigender, and two-spirit. As behavior analysts heed the call to engage in culturally competent practices that address diverse sexual orientation and gender identities (SOGI), they will likely consider collecting SOGI data as part of their practice. The benefits of SOGI data collection certainly exist. However, the historical oppression and increased vulnerability of SGM populations require a careful and thorough evaluation of ethical data collection practices to avoid harm and to ensure respectful and inclusive practices. The present tutorial aims to begin the discussion of ethical and effective SOGI data collection practices within behavior analysis by offering initial guidelines and considerations. We highlight methods that improve cultural sensitivity, and caution against traditional methods that could harm respondents or contribute to a non-inclusive environment.