bluuebunny/crossref_metadata_2025_split huggingface dataset:
Severity and duration of immune response in people of different age categories after SARS-CoV-2 revaccination
Authors: Drapkina O. M. , Berns S. A. , Chashchin M. G. , Gorshkov A. Yu. , Zhdanova O. V. , Ryzhakova L. N. 
Published: February 2024

The article discusses the effectiveness and safety of homologous and heterologous revaccination for Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in people of different age groups.Aim. The study the severity and duration of immune response in people of different age groups after SARS-CoV-2 revaccination.Material and methods. The study was carried out as part of the prospective registry Saturn, which included persons who received various SARS-CoV-2 revaccination regimens (homoand heterologous) based on the combination of two vaccines: Gam-COVID-Vac and Covivac. Group I (n=106) included persons who received a homologous revaccination with Gam-COVID-Vac, while group II (n=54) — heterologous revaccination with Gam-COVID-Vac and Covivac, group III (n=40) — homologous Covivac revaccination. In addition, all participants in the study were divided into two age categories: ≥60 years (n=33) and <60 years, (n=167). In all participants, the medical history was collected. At each visit, we assessed the quantitative level of specific IgG SARS-CoV-2 S-protein antibodies and plasma coagulation using the thrombodynamics method. At visits 1, 3 and 5, blood was collected to assess T-cell immunity activity (T-Spot.COVID test, Oxford Immunotec) to the SARS-CoV-2 virus.Results. Revaccination in persons ≥60 years of age ensures the intensity of humoral (quantitative level of specific IgG SARS-CoV-2 S-protein antibodies) and cellular immunity (quantitative assessment of SARS-CoV-2 spike protein-specific (panel A) and nucleocapsid-specific T-lymphocytes) comparable to that in younger individuals (<60 years). Revaccination did not increase the plasma thrombogenic potential in vaccinated people, regardless of age.Conclusion. In the group of vaccinated people ≥60 years old, an adequate humoral and cellular immune response was developed, which persisted for a year after revaccination, and even more intense cellular immunity was demonstrated compared to vaccinated people <60 years old. The plasma thrombogenic potential did not increase during vaccination and revaccination, regardless of age.
Informationstechnische Standardisierung prozessnaher TGA
Authors: Krüttgen Michael , Penndorf Sebastian , Schauß Jörg , Müller Jochen 
Published: February 2024

Für die informationstechnische Abbildung der prozessnahen TGA und GA fehlen in der Praxis grundlegende Festlegungen und Standards. Mangelnde Zugänglichkeit und Verfügbarkeit standardisierter Informationen erschweren das Engineering und den Betrieb. In 2024 wird die NE196 mit einem Geräteprofil von Digitalen Zwillingen, bezogen auf die Aspekte Prozessführung, Simulation und Plant Asset Management, auf Basis des lebenszyklusübergreifenden Informationsmodells der I4.0-Verwaltungsschale veröffentlicht. Der Aufwand für das Datenhandling wird reduziert und regelbasierte Anwendungen werden möglich.
Evaluation of human mononuclear umbilical cord blood cells systemic administration efficiency in the acute period of experimental severe spinal cord injury
Authors: Smirnov V. A. , Ryabov S. I. , Zvyagintseva M. A. , Bazanovich S. A. , Morozova Ya. V. , Radaev S. M. , Talypov A. E. , Grin A. A. 
Published: February 2024

Aim. To evaluate the efficiency of systemic (intravenous) application of cryopreserved human umbilical cord blood mononuclear cells (HUCBCs) in animal models of acute contusion spinal cord injury for the restoration of hind limb motor function and formation of posttraumatic cysts using clinically significant examination methods.Materials and methods. Adult female Sprague–Dowley rats were used for the study. Severe acute contusion spinal cord injury model was performed using standard “weight‑drop” method. All samples of cryopreserved HUCBCs concentrate were prestored prior to infusion for 3 to 4 years at –196 °C. Hind limbs motor function was evaluated using open‑field technique and standard BBB testing system. Magnetic resonance scanning was performed using high‑field magnetic resonance CleanScan 7.0 T tomography (Bruker BioSpin, Germany).Results. Intravenous infusions of HUCBCs were performed on Day 1 following acute severe spinal cord injury. Motor function assessment demonstrated significant (p <0.05) improvement of hind limbs motor function (up to 40–50 %) comparing to self‑healing outcomes. Moreover, by the Days 4 and 5 after severe spinal cord injury, the volume of posttraumatic cystic cavity decreases significantly (up to 40 %) (p <0.05).Conclusion. The obtained results demonstrated that cryopreserved HUCBCs can be used as an effective source for cell therapy of acute contusion spinal cord injury.
Avaliação da extração sólido-líquido de levodopa a partir de sementes de mucuna utilizando diferentes solventes
Authors: Sousa Rita de Cássia Superbi de , Quieli Giovani da Silva , Monteiro Thais Pinto Coelho , Costa Angélica Ribeiro da , Benfica Jordana 
Published: February 2024

A levodopa é a principal droga utilizada atualmente no tratamento da doença de Parkinson. Esse aminoácido, encontrado na semente de mucuna, é um precursor direto da dopamina, que atua na redução e controle dos sintomas da doença. A levodopa comercializada é produzida de forma sintética, no entanto, devido a relevância do tema, estudos têm sido feitos para encontrar alternativas de extração desse composto a partir de fontes naturais, tornando o processo mais econômico e sustentável com o uso de solventes alternativos. Neste estudo, o objetivo foi caracterizar as sementes de mucuna cinza (Mucuna cinerea) e avaliar o rendimento da extração sólido-líquido de levodopa, utilizando diferentes solventes (ácido benzoico, ácido succínico, cloreto de colina, etanol, PEG 4000 e água). Para avaliar quais solventes e fatores que mais influenciavam no rendimento de extração, um delineamento fatorial (23) foi realizado. Os fatores foram a temperatura, tempo de extração e a razão entre sólido e solvente. A análise da composição mostrou que a mucuna é rica em proteínas com 26,49% e carboidratos com 50,63%, tem 10,16% de minerais, 4,08% de lipídeos e 8,64% de umidade. A quantificação da levodopa foi feita por cromatografia líquida de alta eficiência, sendo mistura aquosa de água e etanol 50% m/m o solvente mais indicado para a extração, alcançando o rendimento de 14,14%.
“She was not an outsider artist”
Authors: Reily Lucia , Röske Thomas 
Published: February 2024

This interview was conducted with Dr. Thomas Röske, director of the Prinzhorn Collection Museum on May 30th, 2023, focusing on three main issues: introducing the artist Elfriede Lohse-Wächtler and her oeuvre in relation to her history as a visual artist in the nineteen-twenties and thirties; discussing her status as an artist affected by psychiatric experience [psychiatrisch Erfahrung], and reporting on the acquisition process of this significant estate for the museum.
Dinâmica tempo-espacial da linha de costa associada a delta de maré vazante, Cacha Pregos e Ponta dos Garcez, Bahia, Brasil
Authors: Nascimento Eduardo Afonso Nunes , Andrade Ana Cláudia da Silva 
Published: February 2024

O trabalho investigou o comportamento da linha de costa em duas praias contíguas a um delta de maré vazante, na Bahia, Brasil. A dinâmica tempo-espacial foi avaliada por meio de dados históricos (imagens de satélite) e sazonais (geoindicadores de campo). A análise qualitativa de médio prazo (1984 a 2020) mostrou que a linha de costa recuou ou progradou em função das mudanças na configuração das barras de embocadura e do canal principal de vazante. A análise quantitativa de médio prazo (2003-2019) ressaltou a importância das células de transporte litorâneo na dispersão de sedimentos ao longo da costa. Na análise de curto prazo (sazonal), em anos sob influência de La Niña (2021-2022), não foi verificada uma tendência no comportamento das linhas de costa estuarinas e oceânica de alta variabilidade. Adicionalmente, foi identificada a presença de um reservatório de sedimentos, de médio e curto prazos, que faz com que os sedimentos recirculem, principalmente nas partes de alta variabilidade. Por fim, a praia estuarina de Cacha Pregos, protegida por estruturas de contenção, apresentou erosão crônica (irreversível). Os demais trechos, em erosão aguda, compreendem áreas sem ocupação, que podem se recuperar naturalmente. O trabalho serve de subsídios ao planejamento ambiental de zonas costeiras.
Flexible Dissolving Microneedle Array for Efficient DNA Vaccination by Electroporation-assisted Transdermal Immunization
Authors: Zhao Huiting , Wang Xu , Ma Tianjiao , Geng Zhanhui 
Published: February 2024

Abstract
DNA vaccination has emerged as an innovative approach for preventing and treating different diseases, including infectious diseases, cancers and autoimmune diseases. Although the results of ongoing clinical trials are promising, several limitations compromise the immunogenicity of these vaccines. Here, we presented a novel immune strategy that combined intradermal immunization with skin electroporation to enhance the immunogenicity of the DNA vaccine. A flexible dissolving microneedle array (FDMNA) was developed, comprising 400 pyramidal microneedles. The microneedle array included dissolving needles for delivering DNA vaccine and a flexible needle substrate for good tissue surface coverage. The FDMNA was completely dissolved within 10 minutes after being inserted into the skin and transported the DNA into the immune-cell-rich epidermis and dermis layer. In vivo immunogenicity test was performed to observe the ability of FDMNA-mediated intradermal electroporation to induce immune response. The electroporation voltage, pulse number and pulse duration were 100 V, 6 pulses and 50 ms, respectively. The result showed that, compared with the intramuscular vaccination, the FDMNA induced higher levels of antibody immune response against ovalbumin in mice. These findings demonstrated the potential utility of a Flexible Dissolving Microneedle Array(FDMNA)-based immune strategy for enhanced DNA vaccination.
Effective radiation dose reduction in total body PET/CT while improving diagnostic value
Authors: Chai Leiying , Cheng Zhaoping , Duan Yanhua , Xu Jiwei , Jing Yudong , Cui Xiao , Li Hui , Li Kun 
Published: February 2024

Abstract

Background
18F-FDG positron emission tomography (PET) combined with computed tomography (CT) has become an indispensable imaging modality in clinical practice. However, the extent of radiation exposure patients receive with an effective radiation dose has always been a concern of radiological protection. The aim of the current study was to estimate the effective doses in total-body PET/CT scans that reasonably reduced the internal radiation-absorbed dose from PET and effectively controlled the external radiation-absorbed dose from CT.
Methods
Patient doses were calculated, including internal radiation-absorbed dose from PET and external radiation-absorbed dose from CT, using a total-body PET/CT scanner.
Results
The results showed an average radiation dose of 2.43 ± 0.36 mSv and 13.08 ± 1.37 mSv for the PET and CT components, respectively, resulting in a total dose of 15.51 ± 1.71 mSv. The diagnostic CT scan protocols contributed 84% of the overall effective radiation doses, which was larger than the percentage contributed by the PET component (16%).
Conclusions
The injection dose was 1.85 MBq/kg, which was decreased to 1/3 that of conventional activity (5.55 MBq/kg). Reducing the diagnostic dose using the technology of automatic exposure control (AEC) will be helpful in decreasing the effective radiation doses of PET/CT scan.
Exploring the Pharmacological Action Mechanism of Chamomile Essential Oil on the Treatment of Breast Cancer Based on Network Pharmacology
Authors: Maitisha Guzhalinuer , Zhou Junhao , Zhao Youyun , Liu Guangzhong , Zhao Yan , Zheng Yi , Li Ling , Han Shuxia , Peng Li , Abliz Ablikim 
Published: February 2024

Abstract
Background
Chamomile essential oil (CEO) can be beneficial in cancer therapy. The aim of the current research is to explore the underlying mechanism of CEO for breast cancer treatment by network pharmacology approach and evaluate its anti-breast cancer capacity in vitro.
Methods
We extracted CEO from chamomile flowers and analyzed its chemical components by using GC-MS/MS. Network pharmacology method was employed to screen the active components, potential targets and possible mechanism of CEO for breast cancer treatment. The molecular docking was used to validated the results of network pharmacology. Cell viability, apoptosis and cell cycle assay were used to assess anti-breast cancer effect of CEO.
Results
In network pharmacology analysis, we found the 12 effective components and 265 drug-disease common targets of CEO and among them, five active components and 19 targets were determined as the therapeutic targets of breast cancer. GO results demonstrated that the potenributetial targets of CEO were primarily participated in positive regulation of MAPK cascade, distd in membrane raft and the molecular functions were associated with protein serine-threonine-tyrosine kinase activity. KEGG pathway analysis suggested that the potential targets mainly involved in PI3K-AKT signaling pathway, cAMP signaling pathway, neuroactive ligand-receptor interaction, MAPK signaling pathway and calcium signaling pathway. Molecular docking analysis revealed that LYN, LCK, VGFR, MAPK11, MAPK14, PTK2, JAK1, NR3C1 and ESR1 have shown higher affinity with three components from CEO and suggesting that these compounds might be the most effective ingredients against breast cancer. Besides, we found that CEO treatment suppressed cell proliferation by inducing cell cycle arrest in breast cancer cell lines.
Conclusions
These findings suggest that CEO inhibit proliferation of breast cancer cell lines through modulating those multi-pathways and multi-targets. The present study not only indicate the therapeutic potential of CEO for breast cancer, but also provide valuable insight into its mechanism of action.
Adenocarcinoma originating in the anterior mediastinum diagnosed by endobronchial ultrasound-guided transbronchial cryobiopsy: A case report
Authors: Tsuchida Hiroyuki , Matsumoto Yuji , Furuse Hideaki , Tsuchida Takaaki 
Published: February 2024

Abstract
Background
Endobronchial ultrasound-guided transbronchial cryobiopsy (EBUS-cryobiopsy) is advantageous for collecting larger specimens with minimal crushing; however, it has not been widely used for mediastinal tumors.
Case presentation:
A 73-year-old woman with a history of left breast cancer underwent surgery, followed by radiotherapy. Computed tomography showed a mass in the anterior mediastinum that was in extensive contact with the sternum on the ventral side and partly with the trachea on the dorsal side. Two computed tomography-guided needle biopsies (CTNBs) were performed on the mass; however, a definitive diagnosis was not made because of severe crush artifacts. Subsequently, we performed EBUS-cryobiopsy and safely obtained sufficient specimen volume with minimal crushing. The histopathological diagnosis was adenocarcinoma, with immunobiological features distinct from those of previous breast cancers. Her overall diagnosis was a rare tumor originating in the anterior mediastinum.
Conclusions
EBUS-cryobiopsy can be safely performed in narrow areas surrounded by major blood vessels, and the obtained specimens may be superior to CTNBs for histopathological diagnosis.
Indoor residual spraying of experimental huts in Cameroon highlights the potential of Fludora® Fusion to control wild pyrethroid-resistant malaria vectors
Authors: Thiomela Riccado F. , Tchouakui Magellan , Menze Benjamin D. , Nchoutpouen Elysee , Ngongang-Yipmo Emilie S. , Wood Oliver , Horstmann Sebastian , Mahob Raymond J. , Fomena Abraham , Wondji Charles S. 
Published: February 2024

Abstract

Elevated resistance to pyrethroids has led to the introduction of novel insecticides including neonicotinoids. However, the efficacy of these new control products could be impacted by cross-resistance mechanisms from metabolic resistance to pyrethroids. In this study, after evaluating the resistance to clothianidin and mixture of clothianidin+deltamethrin in the lab using CDC bottle assays, the efficacy of the new IRS formulation Fludora® Fusion IRS was tested in the experimental huts against wild free-flying pyrethroid-resistant Anopheles funestus in Elende and field An. gambiae from Nkolondom, Cameroon. Additionally, cone tests were performed on the wall of treated huts each month to evaluate the residual efficacy of the sprayed products. Furthermore, the L1014F-kdr target site mutation and the L119F-GSTe2 mediated metabolic resistance to pyrethroids were genotyped on a subset of mosquitoes from the EHT to assess the potential cross-resistance. All Anopheles species tested were fully susceptible to clothianidin and clothianidin+deltamethrin mixture in CDC bottle assay while resistance was noted to deltamethrin. Accordingly, Fludora® Fusion and clothianidin induced significantly higher mortality rates in EHT than deltamethrin with mortality rates of 62.83%, 64.42% and 42.42% against free flying An. funestus from Elende. However, lower mortality rates were recorded against An. gambiae from Nkolondom (mortality rates 50%, 45.56% and 26.68%). In-situ cone test on the wall showed a high residual efficacy of Fludora® Fusion and clothianidin on the susceptible strain KISUMU (>12 months) and moderately on the highly pyrethroid-resistant An. gambiae strain from Nkolondom (6 months). Interestingly, no association was observed between the L119F-GSTe2 mutation and the ability of mosquitoes to survive exposure to Fludora® Fusion, whereas a trend was observed with the L1014F-kdr mutation. This study highlights that Fludora® Fusion, has good potential of controlling pyrethroid-resistance mosquitoes with prolonged residual efficacy. This could be therefore an appropriate tool for vector control in several malaria endemic regions.
Association between urinary albumin-to-creatinine ratio within the normal range and insulin resistance and prediabetes among US adults: a population-based study
Authors: Wu Shuang , Yang Yan-min , Zhu Jun , Wang Lu-lu , Xu Wei , Lyu Si-qi , Wang Juan , Shao Xing-hui , Zhang Han 
Published: February 2024

Abstract

Background Recent studies have shown that urine albumin-to-creatinine ratio (UACR) within the normal range is associated with increased risk of hypertension, diabetes, cardiovascular death and all-cause death. However, the relationship between normal range UACR and the prevalence of insulin resistance (IR) and prediabetes has not been explored.
Methods The study included 14,208 US adults with normal range UACR values and without diabetes from the National Health and Nutrition Survey (1999-2018). The outcome variables included IR and prediabetes, as defined by the 2013 American Diabetes Association guidelines. The weighted multivariate linear regression, weighted multivariate logistic regression, restricted cubic spline (RCS) regression, and subgroup analysis were conducted to examine the relationship of UACR with IR and prediabetes.
Results The weighted multivariate linear regression analysis showed that a 1-standard deviation (SD) increment in ln (UACR) was positively associated with fasting plasma glucose (FPG) (β = 0.02, 95% CI: 0.01, 0.04), glycosylated hemoglobin (β = 0.01, 95% CI: 0.00, 0.02), fasting serum insulin (FSI) (β = 0.50, 95% CI: 0.31, 0.68), and homeostasis model assessment of insulin resistance (HOMA-IR) (β = 0.14, 95% CI: 0.09, 0.19). The weighted multivariate logistic regression analysis revealed that a 1-SD increment in ln (UACR) was significantly associated with an increased prevalence of prediabetes (OR = 1.10, 95% CI: 1.04-1.15), but not with IR (OR = 1.05, 95% CI: 0.99-1.12). With the lowest quartile of UACR as a reference, ORs (95% CIs)for IR in the second, third, and fourth quartiles were 1.10 (0.86, 1.15), 1.15 (0.98, 1.33), and 1.12 (0.95, 1.32), respectively, and ORs (95% CIs) for prediabetes were 1.35 (1.19, 1.52), 1.32 (1.14, 1.53), and 1.44 (1.24, 1.66), respectively. Subgroup analysis indicated that smoking had a significant impact on the association between UACR and prediabetes. The association between UACR and prediabetes was more significant in smokers than in non-smokers (P for interaction = 0.03). RCS regression analysis revealed a non-linear positive dose-response association between UACR and indicators of diabetes.
Conclusion Elevated UACR, even within the normal range, was positively associated with indicators of diabetes and increased prevalence of prediabetes among US adults. Smoking had a significant impact on the association between UACR and prediabetes, with a more pronounced association observed among smokers.
Uninterrupted Growth, Redistribution and Inequality: The Australian Case 1991-2020
Authors: Tran Chung , Zakariyya Nabeeh 
Published: February 2024

Abstract
To what extent can a progressive tax and transfer system moderate the distributional impacts of uneven economic growth? We revisit this question in the unique Australian context of three decades of uninterrupted economic growth from 1991 to 2020. We use longitudinal tax records of millions of Australian taxpayers to examine the distributional impacts of uneven growth and the redistributive role of progressive income taxes and targeted transfers. Our results indicate that the uneven distribution of income growth, favoring higher income groups of taxpayers, shapes a rising trend in market income inequality. The increased progressivity of the tax and transfer system has been instrumental in moderating these uneven gains across dierent groups. While inter-cohort income inequality has risen over time, lifetime inequality within cohorts has remained relatively lower and more stable, revealing potentially biased conclusions drawn from cross-sectional analyses. Employing a dynamic general equilibrium lifecycle model, we highlight the trade-offs between effciency and equity when implementing more progressive tax and transfer policies. Thus, our findings suggest that accounting for taxes and transfers, and lifetime income materially affects measures of inequality.
JEL: E62, H24, H31
Association between thyroid indices and ischemic stroke: Glycated albumin as a mediator
Authors: Jia Ruirui , Wang Hui , Chen Dong , Wang Long , Li Zhaoyang , Zhang Youyou 
Published: February 2024

Abstract

Limited data are available on the association between the thyroid indices and ischemic stroke (IS) risk, and the mechanisms remain unclear. We aimed to investigate the associations of thyroid indices and the prevalence of IS and examine the mediating effects through glycated albumin (GA). A total of 332 patients with ischemic stroke were matched 1:1 to patients without ischemic stroke for age and gender. Serum thyroid indices (free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH) and GA were collected from biochemical test results. Multivariate conditional logistic regression analyses were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for thyroid indices and IS. Generalized linear models were used to analyze the associations between levels of thyroid indices and GA. Mediation analyses were further conducted to evaluate the potential mediating role of GA in the associations between thyroid indices and IS. Each SD increment of FT4 was associated with 26% higher odds (OR: 1.26; 95%CI: 1.05, 1.51) increment in ORs of IS. However, each SD increment of FT3 or FT3/FT4 ratio was associated with 20% lower odds (OR: 0.80; 95%CI: 0.67, 0.96) and 30% lower odds (OR 0.70; 95%CI: 0.58, 0.85) decrements in ORs of IS, respectively. Further mediation analyses indicated that GA mediated 18.16% and 11.35% of the associations between FT3 and FT3/FT4 ratio and IS, respectively. Higher serum FT4 or lower serum FT3 and FT3/FT4 ratio was associated with an increased risk of IS. The association of FT3 and FT3/FT4 ratio with IS was partially mediated by GA. Further large prospective studies are warranted to corroborate our findings.