bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and characterization of polymorphic microsatellite loci for the three Iberian vipers, Vipera aspis, V. latastei and V. seoanei by Illumina MiSeq sequencing
Authors: Freitas Inês , Velo-Antón Guillermo , Lopes Susana , Muñoz-Merida Antonio , Martínez-Freiría Fernando 
Published: February 2024

Abstract

Background
European vipers (genus Vipera) are a well-studied taxonomic group, but the low resolution of nuclear sanger-sequenced regions has precluded thorough studies at systematic, ecological, evolutionary and conservation levels. In this study, we developed novel microsatellite markers for the three Iberian vipers, Vipera aspis, V. latastei and V. seoanei, and assessed their polymorphism in north-central Iberian populations.

Methods and results
Genomic libraries were developed for each species using an Illumina Miseq sequencing approach. From the 70 primer pairs initially tested, 48 amplified reliably and were polymorphic within species. Cross-species transferability was achieved for 31 microsatellites loci in the three target species and four additional loci that were transferable to one species only. The 48 loci amplified in average seven alleles, and detected average expected and observed heterozygosities of 0.7 and 0.55, in the three genotyped populations/species (26 V. aspis, 20 V. latastei and 10 V. seoanei).

Conclusions
Our study provides a selection of 48 polymorphic microsatellite markers that will contribute significantly to current knowledge on genetic diversity, gene flow, population structure, demographic dynamics, systematics, reproduction and heritability in these species, and potentially in other congeneric taxa.
The Effect of Carbon Structure of DLC Coatings on Friction Characteristics of MoDTC-Derived Tribofilm by Using an In Situ Reflectance Spectroscopy
Authors: Hashizume Naoya , Yamamoto Yusei , Chen Cheng , Tokoroyama Takayuki , Zhang Ruixi , Diao Dongfeng , Umehara Noritsugu 
Published: February 2024

AbstractIn this study, six types of DLC coatings were prepared, featuring different carbon structures (including amorphous ta-C coatings and GNC coatings with nanocrystallites) and different doped Ta amounts, to investigate friction characteristics. The results of friction tests with MoDTC-added lubricant revealed a consistent trend: DLC coatings with a higher ID/IG ratio exhibited lower friction coefficients. In addition, in situ observations using reflectance spectroscopy highlighted that the tribofilm formed on DLC coatings with a higher ID/IG ratio maintained a higher $${{\text{MoS}}}_{2}/({{\text{MoS}}}_{2}+{{\text{MoO}}}_{3})$$

MoS
2

/

(

MoS
2

+

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3

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ratio, which exhibited a strong correlation with the friction coefficient. Measurements of a work function of each DLC coating indicated that those with a higher ID/IG ratio had a higher work function, suggesting the inclusion of a larger amount of graphite structure defects. These active defects in the graphite structure were deemed responsible for enhancing the friction reduction effect of MoDTC. The outcomes of this study propose a material design approach for DLC coatings that amplifies the effectiveness of lubricant additives in friction reduction.
Graphical abstract
Study of secondary dentine deposition in central incisors as an age estimation method for adults
Authors: Marques-Moura S. , Caldas I. M. 
Published: February 2024

Abstract
This work aimed to assess the pulp/tooth area ratio’s utility in the upper central incisors using orthopantomograms. A convenience sample of 801 adult patient orthopantomograms was studied. Image J® software was used to measure the pulp/tooth area ratio, and a regression model was developed. Our results conclude that the methodology assessing upper incisors’ pulp/tooth area ratio using orthopantomograms can lead to age overestimation and statistically significant differences between chronological and estimated age. For those over 50, no correlation between pulp/tooth area ratio and chronological age was found, suggesting that this may be the upper limit of this technique in this population. This methodology may not be suitable for age estimation, particularly in older adults.
Safety profile of trastuzumab deruxtecan in advanced breast cancer: Expert opinion on adverse event management
Authors: Ciruelos Eva , García-Sáenz Jose Ángel , Gavilá Joaquín , Martín Miguel , Rodríguez César A. , Rodríguez-Lescure Álvaro 
Published: February 2024

AbstractTrastuzumab deruxtecan (T-DXd) is an antibody–drug conjugate that targets human epidermal growth factor receptor 2 (HER2) and has shown promising results in the treatment of advanced/metastatic breast cancer. The objective of this report is to provide guidance on the prophylaxis, monitoring, and management of adverse events (AEs) in patients with breast cancer treated with T-DXd, and to emphasize that proper management of AEs is needed to optimize the effectiveness of T-DXd treatment and reduce the number of discontinuations. The article covers various aspects of T-DXd treatment, including its clinical efficacy, safety profile, and dosing considerations, and provides practical recommendations for managing AEs, such as nausea/vomiting, interstitial lung disease, and hematologic toxicity. Although there are still many knowledge gaps about the cause and incidence of AEs in real-world patients, this document may serve as a valuable resource for clinicians who are involved in the care of breast cancer patients receiving T-DXd treatment.
Cerebellar Heterotopia: Broadening the Neuroradiological Spectrum of KBG Syndrome
Authors: Carrara Adelaide , Mangiarotti Camilla , Pasca Ludovica , Politano Davide , Abrusco Fulvio D.’ , Barbero Veronica Carmen , Carpani Adriana , Borgatti Renato , Pichiecchio Anna , Valente Enza Maria , Romaniello Romina 
Published: February 2024

AbstractKBG syndrome is a rare genetic disorder caused by heterozygous pathogenic variants in ANKRD11. Affected individuals have developmental delay, short stature, characteristic facial features, and other dysmorphic findings. To date, a spectrum of unspecific neuroradiological defects has been reported in KBG patients, such as cortical defects, white matter abnormalities, corpus callosum, and cerebellar vermis hypoplasia.Deep clinical and neuroradiological phenotyping and genotype of a patient presenting with mild cognitive and behavioral problems were obtained after written informed consent.We herein describe the first KBG patient presenting with cerebellar heterotopia, a heterogeneous malformation characterized by the presence of clusters of neurons within the white matter of cerebellar hemispheres.This novel association broadens the neuroradiological spectrum of KBG syndrome, and further prompts to investigate the potential functions of ANKRD11 in cerebellar development.
Purification of lipid oil using ion exchange resins
Authors: Selimi Jon , Langschwager Fanny , Tunå Per , Hulteberg Christian P. , Abdelaziz Omar Y. 
Published: February 2024

Abstract
Oil upgrading technologies for fuel production from second-generation feedstocks require pretreatments, such as the removal of ash metals in crude oil, to improve the catalytic performance in the process. The aim of this work was to purify lipid crude oil, derived from black soldier fly larvae (BSFL), from calcium by dry washing using ion exchange resins. Commercially available ion exchange resins suitable for organic liquids, namely GF 202 and Amberlyst 15DRY (AL 15), were selected for the purification experiments. The lipid material dissolved in nonanoic acid in different concentrations of mixtures was passed through a resin-filled plug-flow reactor at 50 °C and 75 °C with a liquid hourly space velocity (LHSV) of 4 h−1. The oil samples were analyzed for calcium using inductively coupled plasma-optical emission spectrometry, while the resin surfaces were examined by scanning electron microscopy. AL 15 showed a better overall performance and led to a case where over 99% of calcium was removed.

Graphical abstract
Quality of life, household income, and dietary habits are associated with the risk of sarcopenia among the Chinese elderly
Authors: Wan Hua , Hu Yan-Hui , Li Wei-Peng , Wang Quan , Su Hong , Chenshu Jun-Yan , Lu Xiang , Gao Wei 
Published: February 2024

Abstract
Background
Health-related quality of life (HRQoL), which can be influenced by various aspects, especially socioeconomic status and lifestyle, has been identified as an important predictor of the prognosis of older adults. Dietary habit, a major part of lifestyle, can affect the nutritional status, which is closely correlated with the development of geriatric syndromes in the elderly.

Aims
The aim of the study was to examine the association of HRQoL, socioeconomic status, and lifestyle with the risk and severity of sarcopenia, a geriatric syndrome characterized by progressive loss of skeletal muscle mass, strength and function.

Methods
A cross-sectional retrospective study with 2877 participants aged ≥65 years was performed. HRQoL was assessed using EuroQoL Five Dimensions questionnaire. Socioeconomic status was assessed by the educational attainment, occupation, and household income. Lifestyle was assessed using 12 items closely related to Chinese living habits. The information of daily dietary habits including tea, alcohol, type of diet, and volume of drinking water were collected. The associations of HRQoL, socioeconomic status, and lifestyle with the risk of sarcopenia were examined by multivariate regression logistical analysis. The potential causal role of age, body mass index, and waist circumference in the effect of HRQoL on sarcopenia risk was analyzed by causal mediation analysis.

Results
High HRQoL [adjusted odds ratio (OR) =0.85, 95% confidence interval (CI) =0.69–0.95, P=0.034] and household income levels (adjusted OR =0.74, 95% CI =0.57–0.95, P=0.019) were inversely associated with the risk of sarcopenia. Meanwhile, more consumption of spicy food (adjusted OR =1.34, 95% CI =1.09–1.81, P =0.037) and occasionally drinking (adjusted OR =1.46, 95% CI =1.07–2.00, P =0.016, as compared to those never drinking) were associated with higher risk of sarcopenia, while skipping breakfast occasionally (adjusted OR =0.37, 95% CI =0.21–0.64, P <0.001, as compared to those eating breakfast every day) and less consumption of salt (adjusted OR =0.71, 95% CI =0.52–0.96, P =0.026, as compared to those consuming high amount of salt) were associated with lower risk of sarcopenia. Further causal mediation analysis aimed to explore how much age, body mass index, and waist circumference might explain the effect of HRQoL on the risk of sarcopenia showed that the estimated proportion that mediated the effect of HRQoL on the risk of sarcopenia by age was 28.0%.

Conclusions
In summary, our findings demonstrate that low levels of HRQoL and household income, more intake of salt and spicy food, and occasional intake of alcohol are correlated with higher risk of sarcopenia, while skipping breakfast occasionally is associated with lower risk of sarcopenia in a Chinese population of older adults.
Longitudinal changes of the femoral bone mineral density from first to third trimester of pregnancy: bone health assessment by means of non-ionizing REMS technology
Authors: Ramirez Zegarra Ruben , Degennaro Valentina , Brandi Maria Luisa , Cagninelli Greta , Casciaro Sergio , Celora Gabriella , Conversano Francesco , Lombardi Fiorella A. , Pisani Paola , Ghi Tullio 
Published: February 2024

Abstract
Background
Throughout the pregnancy, there is a substantial transfer of calcium from the maternal skeleton to the fetus, which leads to a transient net reduction of the maternal bone mineral density.

Aims
To assess longitudinally the changes in the bone mineral density at the femoral neck between the first and third trimester of pregnancy in a cohort of healthy participants using Radiofrequency Echographic Multi Spectrometry (REMS) technology.

Methods
Prospective, cohort study conducted at the University hospital of Parma, Italy between July 2022 and February 2023. We recruited healthy participants with an uncomplicated singleton pregnancy before 14 completed weeks of gestation. All included participants were submitted to a sonographic examination of the femoral neck to assess the bone mineral density (and the corresponding Z-score values) using REMS at 11–13 and 36–38 weeks of pregnancy. The primary outcome was the change in the bone mineral density values at the maternal femoral neck between the first and third trimester of pregnancy.

Results
Over a period of 7 months, a total of 65 participants underwent bone mineral density measurement at the femoral neck at first and third trimester of the pregnancy using REMS. A significant reduction of the bone mineral density at the femoral neck (0.723 ± 0.069 vs 0.709 ± 0.069 g/cm2; p < 0.001) was noted with a mean bone mineral density change of − 1.9 ± 0.6% between the first and third trimester of pregnancy. At multivariable linear regression analysis, none of the demographic or clinical variables of the study population proved to be independently associated with the maternal bone mineral density changes at the femoral neck.

Conclusions
Our study conducted on a cohort of healthy participants with uncomplicated pregnancy demonstrates that there is a significant reduction of bone mineral density at femoral neck from early to late gestation.
Gender Differences in Early Child Development: Evidence from Large-Scale Studies of Very Young Children in Nine Countries
Authors: Bando Rosangela , Lopez-Boo Florencia , Fernald Lia , Gertler Paul , Reynolds Sarah 
Published: February 2024

AbstractSome evidence suggests that there are significant gender gaps in early child development in low- and middle-income countries, with girls generally outperforming boys. However, few studies have tested for the existence of such gaps at a large scale. Our objective is to examine gender disparities in early child development in Brazil, Chile, Colombia, India, Indonesia, Nicaragua, Peru, Senegal, and Uruguay, with 26,055 children aged 7 to 48 months. We estimate gaps using cross-sectional studies with language, socioemotional, and motor skills development assessments. Consistent with small-sample findings, the data shows girls consistently outperformed boys on language tests (0.14 standard deviations) and socioemotional development (0.17 standard deviations), with differences consistent across all nine countries. There were no systematic differences by gender for motor development. We explored how family characteristics, health investments, or parent–child interactions influenced the gap. We did not find evidence that variation on these characteristics across children explained the gap. Our findings suggest that gender gaps in language and socioemotional development emerge very early in life.
Environmental implications of residual pyrogenic carbonaceous materials from incomplete biomass combustion: a review
Authors: Chang Zhaofeng , Shen Guofeng , Jiang Ke , Huang Wenxuan , Zhao Jinfeng , Luo Zhihan , Men Yatai , Xing Ran , Zhao Nan , Pan Bo , Xing Baoshan , Tao Shu 
Published: February 2024

Abstract Incomplete biomass burning produces considerable amounts of pyrogenic carbonaceous materials (PCMs), which are widely distributed in environmental matrices. Those PCMs undergo different environmental processes and consequently have non-negligible impacts on the global carbon cycle, ecological functions and environmental security. This review provided a comprehensive review of qualitative and quantitative methods, carbon sequestration capabilities and other ecosystem functions of PCMs. In addition, the generation mechanism and environmental health risks of emerging contaminants, especially persistent free radicals (EPFRs) and polycyclic aromatic hydrocarbons (PAHs) associated with PCMs were discussed. The results showed that the coexisting kerogen and coal may interfere with PCMs quantification, and that estimates of PCMs pools vary significantly due to methodological differences, natural variability and limited spatial coverage. The input of PCMs into soils increased soil carbon sequestration through direct carbon contribution and indirect negative priming effect on native SOC. In addition, PCMs can improve soil structure and properties and immobilize/degrade pollutants, which is conducive to the restoration of soil ecology. However, various contaminants associated with PCMs may threaten ecological safety, and thus their formation mechanisms and toxicological pathway to living organisms need to be further investigated. The development of standards for PCMs identification and quantification, application protocols of PCMs in pilot scale, and assessing the effects of PCMs on soil health deserve extended studies.
Mapping the Ophiocordyceps sinensis value chain: actors, profits and social institutions in south-west China
Authors: Fan Ben , He Jun 
Published: February 2024

AbstractCommercial use of wildlife is booming. However, knowledge of trade, price structure and benefit distribution mechanisms remain limited for many endemic, threatened and economically valuable species, impeding efforts to develop conservation intervention schemes in line with sustainable objectives. We illustrate the nature of commercialization of the caterpillar fungus Ophiocordyceps sinensis, collected in a high-altitude Tibetan region. Using the value-chain approach, we document how caterpillar fungus is collected in a remote mountainous region and traded across a wide range of middlemen until it finally reaches high-end markets in urban centres. Mapping the caterpillar fungus value chain helps identify the actors involved and the social institutions (i.e. the rules, norms and arrangements that shape people's behaviours) facilitating access to markets and influencing profit distribution and price structure. We present the complex dynamics of this commercialization process, providing a holistic value-chain analysis that encompasses actors, profits and institutions.
The Influence of Future Changes in Tidal Range, Storm Surge, and Mean Sea Level on the Emergence of Chronic Flooding
Authors: Hague Ben S. , Talke Stefan. A. 
Published: February 2024

AbstractSea‐level rise is leading to increasingly frequent coastal floods globally. Recent research shows that changes in tidal properties and storm surge magnitudes can further exacerbate sea‐level rise‐related increases in flood frequencies. However, such non‐stationarity in tide and storm surge statistics are largely neglected in existing coastal flood projection methodologies. Here we develop a framework to explore the effect that different realizations of various sources of uncertainty have on projections of coastal flood frequencies, including changes in tidal range and storminess. Our projection methodology captures how observed flood rates depend on how storm surges coincide with tidal extremes. We show that higher flood rates and earlier emergence of chronic flooding are associated with larger sea‐level rise rates, lower flood thresholds, and increases in tidal range and skew surge magnitudes. Smaller sea‐level rise rates, higher flood thresholds and decreases in sea level variability lead to commensurately lower flood rates. Percentagewise, changes in tidal amplitudes generally have a much larger impact on flood frequencies than equivalent percentagewise changes in storm surge magnitudes. We explore several implications of these findings. Firstly, understanding future local changes in storm surges and tides is required to fully quantify future flood hazards. Secondly, existing hazard assessments may underestimate future flood rates as changes in tides are not considered. Finally, identifying the flood frequencies and severities relevant to local coastal managers is imperative to develop useable and policy‐relevant projections for decisionmakers.
Overriding Plate Thickness as a Controlling Factor for Trench Retreat Rates in Narrow Subduction Zones
Authors: Gea Pedro J. , Mancilla Flor de Lis , Negredo Ana M. , van Hunen Jeroen 
Published: February 2024

AbstractSlab width is a significant factor in controlling subduction zone dynamics, particularly the retreat velocities, which tend to decrease with wider slabs. However, observations of natural narrow subduction zones reveal no correlation between slab width and trench velocities. This suggests that other factors may exert a greater influence. In this study, we employ 3D numerical subduction models to systematically assess the impact of slab width, strength of slab coupling to the lateral plate (LP), and overriding plate (OP) thickness on trench kinematics and geometry. Our models focus on narrow slabs (400–1,200 km), and the results demonstrate that, in the case of narrow subduction zones, the slab width has little effect on trench migration rates and the viscous coupling at the lateral slab edge is only important for very narrow subduction zones (≤800 km). Conversely, the OP thickness emerges as a crucial factor, with increasing plate thickness leading to a strong decrease in trench velocities. These findings provide an explanation for the observed trench velocities in natural narrow subduction zones, where an inverse relationship with OP thickness is evident. Furthermore, our study reveals that not only slab width, but also the OP thickness and the slab coupling to the LP, significantly influence trench geometry. Strong lateral coupling promotes the formation of concave trench geometries, while thick overriding plates favor the development of “w”‐shaped geometries. Overall, a comprehensive understanding of subduction processes necessitates considering the interplay between slab width, OP thickness, and slab coupling to the LP.
Social isolation and aggression training lead to escalated aggression and hypothalamus-pituitary-gonad axis hyperfunction in mice
Authors: Oliveira Vinícius Elias de Moura , Evrard Florence , Faure Melanie C. , Bakker Julie 
Published: February 2024

AbstractAlthough the participation of sex hormones and sex hormone-responsive neurons in aggressive behavior has been extensively studied, the role of other systems within the hypothalamus-pituitary-gonadal (HPG) axis remains elusive. Here we assessed how the gonadotropin-releasing hormone (GnRH) and kisspeptin systems are impacted by escalated aggression in male mice. We used a combination of social isolation and aggression training (IST) to exacerbate mice’s aggressive behavior. Next, low-aggressive (group-housed, GH) and highly aggressive (IST) mice were compared regarding neuronal activity in the target populations and hormonal levels, using immunohistochemistry and ELISA, respectively. Finally, we used pharmacological and viral approaches to manipulate neuropeptide signaling and expression, subsequently evaluating its effects on behavior. IST mice exhibited enhanced aggressive behavior compared to GH controls, which was accompanied by elevated neuronal activity in GnRH neurons and arcuate nucleus kisspeptin neurons. Remarkably, IST mice presented an increased number of kisspeptin neurons in the anteroventral periventricular nucleus (AVPV). In addition, IST mice exhibited elevated levels of luteinizing hormone (LH) in serum. Accordingly, activation and blockade of GnRH receptors (GnRHR) exacerbated and reduced aggression, respectively. Surprisingly, kisspeptin had intricate effects on aggression, i.e., viral ablation of AVPV-kisspeptin neurons impaired the training-induced rise in aggressive behavior whereas kisspeptin itself strongly reduced aggression in IST mice. Our results indicate that IST enhances aggressive behavior in male mice by exacerbating HPG-axis activity. Particularly, increased GnRH neuron activity and GnRHR signaling were found to underlie aggression whereas the relationship with kisspeptin remains puzzling.