bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis of green and purple fruited pepper provides insight into novel regulatory genes in anthocyanin biosynthesis
Authors: Tan Huaqiang , Li Liping , Tie Manman , Lu Ronghai , Pan Shaokun , Tang Youwan 
Published: January 2024

Background
Pepper (Capsicum annuum L.) is a valuable horticultural crop with economic significance, and its purple fruit color is attributed to anthocyanin, a phytonutrient known for its health-promoting benefits. However, the mechanisms regulating anthocyanin biosynthesis in pepper have yet to be fully elucidated.

Methods
RNA sequencing (RNA-seq) was utilized to analyze the transcriptome of fruits from three purple-fruited varieties (HN191, HN192, and HN005) and one green-fruited variety (EJT) at various developmental stages. To determine the relationships between samples, Pearson correlation coefficients (PCC) and principal component analysis (PCA) were calculated. Differential expression analysis was performed using the DESeq2 package to identify genes that were expressed differently between two samples. Transcription factors (TF) were predicted using the iTAK program. Heatmaps of selected genes were generated using Tbtools software.

Results
The unripe fruits of HN191, HN192, and HN005, at the stages of 10, 20, and 30 days after anthesis (DAA), display a purple color, whereas the unripe fruits of variety EJT remain green. To understand the molecular basis of this color difference, five transcriptome comparisons between green and purple fruits were conducted: HN191-10 vs EJT-10, HN191-20 vs EJT-20, HN191-30 vs EJT-30, HN192-30 vs EJT-30, and HN005-30 vs EJT-30. Through this analysis, 503 common differentially expressed genes (DEGs) were identified. Among these DEGs, eight structural genes related to the anthocyanin biosynthesis pathway and 24 transcription factors (TFs) were detected. Notably, one structural gene (MSTRG.12525) and three TFs (T459_25295, T459_06113, T459_26036) exhibited expression patterns that suggest they may be novel candidate genes involved in anthocyanin biosynthesis. These results provide new insights into the regulation of anthocyanin biosynthesis in purple pepper fruit and suggest potential candidate genes for future genetic improvement of pepper germplasm with enhanced anthocyanin accumulation.
Prognostic significance of integrating total metabolic tumor volume and EGFR mutation status in patients with lung adenocarcinoma
Authors: Jiang Maoqing , Guo Xiuyu , Chen Ping , Zhang Xiaohui , Gao Qiaoling , Zhang Jingfeng , Zheng Jianjun 
Published: January 2024

Background
The objective of this study was to investigate the prognostic significance of total metabolic tumor volume (TMTV) derived from baseline 18F-2-fluoro-2-deoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT), in conjunction with epidermal growth factor receptor (EGFR) mutation status, among patients with lung adenocarcinoma (LUAD).

Methods
We performed a retrospective analysis on 141 patients with LUAD (74 males, 67 females, median age 67 (range 34–86)) who underwent 18F-FDG PET/CT and had their EGFR mutation status determined. Optimal cutoff points for TMTV were determined using time-dependent receiver operating characteristic curve analysis. The survival difference was compared using Cox regression analysis and Kaplan‒Meier curves.

Results
The EGFR mutant patients (n = 79, 56.0%) exhibited significantly higher 2-year progression-free survival (PFS) and overall survival (OS) rates compared to those with EGFR wild-type (n = 62, 44.0%), with rates of 74.2% vs 69.2% (P = 0.029) and 86.1% vs 67.7% (P = 0.009), respectively. The optimal cutoff values of TMTV were 36.42 cm3 for PFS and 37.51 cm3 for OS. Patients with high TMTV exhibited significantly inferior 2-year PFS and OS, with rates of 22.4% and 38.1%, respectively, compared to those with low TMTV, who had rates of 85.8% and 95.0% (both P < 0.001). In both the EGFR mutant and wild-type groups, patients exhibiting high TMTV demonstrated significantly inferior 2-year PFS and OS compared to those with low TMTV. In multivariate analysis, EGFR mutation status (hazard ratio, HR, 0.41, 95% confidence interval, CI [0.18–0.94], P = 0.034) and TMTV (HR 8.08, 95% CI [2.34–28.0], P < 0.001) were independent prognostic factors of OS, whereas TMTV was also an independent prognosticator of PFS (HR 2.59, 95% CI [1.30–5.13], P = 0.007).

Conclusion
Our study demonstrates that the integration of TMTV on baseline 18F-FDG PET/CT with EGFR mutation status improves the accuracy of prognostic evaluation for patients with LUAD.
Successfully Completed Laparoscopic Splenectomy for Young Woman With Immune Thrombocytopenic Purpura During Pregnancy in Reduced Port Surgery: A Case Study
Authors: Baba Shigeaki , Sasaki Akira , Otsuka Koki , Umemura Akira , Takahara Takeshi , Akiyama Yuji , Iwaya Takeshi , Nitta Hiroyuki , Koeda Keisuke , Kimura Yusuke 
Published: January 2024

Background
Immune thrombocytopenic purpura (ITP) during pregnancy is a rare condition; however, it can lead to hemorrhagic complications due to gestational thrombocytopenia during delivery. A splenectomy is the treatment that will result in the highest complete remission rate for ITP and is sometimes required when thrombocytopenia is severe and fails to respond to medical treatment. Herein, we present a case of a patient who underwent a reduced port laparoscopic splenectomy (RPLS) for ITP during pregnancy.

Case presentation
A 33-year-old female patient, who had past history of miscarriage at the 12th week of gestation and a stillbirth at the 36th week of gestation, was diagnosed with ITP at the 12th week of gestation. The patient received medical treatment, but the platelet count did not increase enough for spontaneous delivery. Therefore, a 3-port RPLS was scheduled at the 20th week of gestation following treatment with high-dose immunoglobulin therapy for 5 days.
The patient was placed in the right semilateral position. The pneumoperitoneal pressure was set at 8 mmHg, which is lower than that used in a typical operation. The overall procedures were similar to those of a 4-port technique.

Results
There was no change in the fetal heart rate during or after the operation. The platelet counts increased and were maintained after the RPLS, and the course of the pregnancy was successful. The patient was able to deliver a healthy infant via vaginal delivery at the 37th week of gestation.

Conclusion
In our experience, RPLS procedures offer high cosmetic satisfaction and are feasible and safe during the second trimester in pregnant women with ITP that does not respond well to medical treatments.
Weathering of granitic rocks by chlorides: effect of the nature of the solution on weathering morphology
Authors: Rivas T. , Prieto B. , Silva B. , Birginie J. M. 
Published: April 2003

AbstractFive granites from NW Spain were subjected to two salt‐spray weathering tests under controlled atmospheric conditions. Granite samples were exposed to a sodium chloride solution in the first test and to a complex solution (sea water) in the second. Subsequent examination of the rocks by light and petrographic microscopy clearly demonstrated the development of different weathering morphologies in each test. The distribution of dissolved ions in samples taken at different depths from the weathered surfaces at the end of the experiments, and the changes in weight of the samples during the tests, also differed. In our opinion, these results were determined by the nature of the solution used; although sodium chloride is the predominant salt in sea water, the presence of other ions modifies its solubility, thereby varying the dynamics of mobility and precipitation and thus the weathering morphology that develops. Copyright © 2003 John Wiley & Sons, Ltd.
Biologically Active Polymers, 6
Authors: Kenawy El‐Refaie , Mahmoud Yehia A.‐G. 
Published: April 2003

AbstractVarious copolymers were prepared by the copolymerization of 2‐chloroethyl vinyl ether (CEVE) with methyl methacrylate (MMA), hydroxyethyl methacrylate (HEMA) and vinylbenzyl chloride (VBC). The copolymers were further modified by quaternization with triethylamine, triphenylphosphine, and tributylphosphine. The antimicrobial activities of the prepared, quaternized copolymers were evaluated against Candida albicans, Fusarium oxysporium, Aspergillus flavus, Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. The antimicrobial activity was explored by the cut plug method, viable cell counting (surviving ratio), transmission electron microscopy, and potassium leakage tests. The results indicated that the prepared polymers had a high antimicrobial activity, and control experiments on the main polymer without ammonium, phenyl, or butyl and/or phosphonium groups were carried out. The phosphonium containing polycationic biocides are more effective than the quaternary ammonium salt polymers. Examining the C. albicans and S. aureus polymer‐treated cells by electron microscopy indicated disruption for the cytoplasmic membrane and release of potassium ion as shown by the assay of potassium leakage.Growth inhibition of different concentrations of polymer (Xb).magnified imageGrowth inhibition of different concentrations of polymer (Xb).
Comparison results for nonlinear elliptic equations with lower–order terms
Authors: Ferone Vincenzo , Messano Basilio 
Published: April 2003

AbstractWe consider a solution u of the homogeneous Dirichlet problem for a class of nonlinear elliptic equations in the form A(u) + g(x, u) = f, where the principal term is a Leray–Lions operator defined on $ W ^{1, p} _{0} (\Omega) $ and g(x, u) is a term having the same sign as u and satisfying suitable growth assumptions. We prove that the rearrangement of u can be estimated by the solution of a problem whose data are radially symmetric.
Macromonomer Chain Growth in the Radical Polymerization of MMA by Cobalt(II) Catalyzed Chain Transfer
Authors: Li Yunying , Wayland Bradford B. 
Published: April 2003

AbstractProperties of the radical polymerization of methyl methacrylate in the presence of cobalt(II) tetraphenylporphyrin ((TPP)CoII•) as a chain transfer catalyst are reported. Reversible addition of (L)Co‐H to macromonomers gives living character to the oligomer olefins that grow to higher molecular weight without altering the primary polymer structural features. Incorporation of reversible termination of oligomer radicals in the mechanistic model for chain transfer catalysis by (L)CoII• complexes is an essential feature of the catalytic chain transfer process.
Average degree of polymerization as a function of monomer conversion for the (TPP)CoII• mediated CCT polymerization of MMA in C6D6 initiated by AIBN at different concentrations of (TPP)CoII•.magnified imageAverage degree of polymerization as a function of monomer conversion for the (TPP)CoII• mediated CCT polymerization of MMA in C6D6 initiated by AIBN at different concentrations of (TPP)CoII•.
Steric‐Factor‐Directed Alternating Supramolecular Copolymer Composed of Hydrogen‐Bonded Cyclohexanetricarboxamide Units
Authors: Takasawa Ryoichi , Murota Kazutoshi , Yoshikawa Isao , Araki Koji 
Published: April 2003

AbstractHydrogen‐bonded supramolecular copolymers were easily prepared by mixing cyclohexanetricarboxamides with three (1) and six (2) alkylsilyl groups, and supramolecular fibers were fabricated. When the composition of 1 and 2 was at or close to equimolarity, the supramolecular copolymer chain was found to have an alternating sequence. This was attributed to the fact that the steric factor of the alkylsilyl side chains effectively controls the unit sequence of the supramolecular polymer chain.magnified image
Vibration analysis and optimization of a primary circuit of the nuclear power plant
Authors: Dupal J. 
Published: April 2003

AbstractThe mathematical model of the primary circuit of a nuclear power plant VVER 420/213 has been developed. The aim of modelling is a vibration analysis and parameter optimization leading to the decrease of velocity and stress amplitudes of the circuit components. For an assemblage of the mathematical model of the whole primary circuit the modal synthesis method with significant DOF reduction is used.
Computation of Controllability and Observability Matrices – Duality and Automatic Differentiation
Authors: Röbenack K. 
Published: April 2003

AbstractDuring the last decades, many new methods for controller and observer design for nonlinear state‐space systems have been developed. Several of these design methods require Lie derivaties and Lie brackets. These derivatives are also needed to compute controllability and observability matrices of nonlinear systems. Up to now, these derivatives have been computed symbolically. An alternative approach based on automatic differentiation is presented.
Modellierung eines elastischen Roboterarmes
Authors: Krajcin Idriz 
Published: April 2003

AbstractIn diesem Beitrag wird eine Modellierung für einen hydraulisch angetriebenen Roboterarm vorgestellt. Die Modellierung beinhaltet sowohl die nichtlineare Eigendynamik eines Differenzialzylinders als auch die elastische Verformung des Roboterarmes, die mit Hilfe der Methode der Finiten Elemente modelliert wird. Mit dem vorgestellten Modell können verschiedene Strategien zur Regelung in Simulationen erprobt und verglichen werden. Die erprobten Regelungsstrategien können dann an dem Laborversuchsträger HyRob, der aus einem hydraulischen Antrieb und einem Arm aus Federrundstahl besteht, untersucht werden.
Behandlung der Dynamik von Eisenbahnschotter mittels Molekulardynamischer Simulation
Authors: Kruse Holger , Popp Karl 
Published: April 2003

AbstractDie Molekulardynamische Simulation stellt ein leistungsstarkes Werkzeug zur Behandlung von granularen Medien wie dem Eisenbahnschotter dar. Im Gegensatz zu einer Kontinuumsbeschreibung wird jeder einzelne Schotterstein vom Modell erfasst. Durch Wahl geeigneter Kontaktgesetze können sowohl kurzzeitdynamische Vorgänge als auch das Setzungsverhalten qualitativ gut abgebildet werden. Interessante Einsatzgebiete sind u. a. eine Optimierung des Schottergleises hinsichtlich der Schwellengeometrie, der Untergrundsteifigkeit oder der Schottersteinform, eine Verbesserung von Stopfverfahren sowie die Berechnung von Verschiebewiderständen.
Microscopic and macroscopic modelling of foams
Authors: Diebels Stefan , Steeb Holger , Ehlers Wolfgang 
Published: April 2003

AbstractThe macroscopic behaviour of foams is influenced by the size and structure of the pores. Therefore, a Cosserat theory is motivated by a micromechanical beam model. A homogenization strategy is applied to the micromechanical model leading to results that are comparable to the predictions of the macroscopic Cosserat model.
Formulierung anisotroper, elektro‐mechanisch gekoppelter Materialgleichungen im Rahmen der Invariantentheorie
Authors: Romanowski H. , Schröder J. 
Published: April 2003

AbstractDieser Beitrag behandelt die Konstruktion anisotroper, elektro‐mechanisch gekoppelter Konstitutivgleichungen für geometrisch lineare Probleme. Hierzu führen wir die elektrische Enthalpie ein, die eine Potentialfunktion für die Spannungen und die elektrische Verschiebung darstellt. Als Modellproblem betrachten wir die Elektrostriktion für den Fall der transversalen Isotropie im Rahmen einer koordinateninvarianten Darstellung.
Experimentelle Untersuchung und thermomechanische Modellierung des Materialverhaltens von Gedächtnislegierungen
Authors: Helm D. , Haupt P. 
Published: April 2003

AbstractAuf der Basis ein‐ und zweiaxialer Experimente an NiTi Formgedächtnislegierungen [1] wird ein geometrisch lineares Materialmodell für Formgedächtnislegierungen vorgestellt. Das Materialmodell basiert auf einer Materialfunktion für die Freie Energie sowie Evolutionsgleichungen für Innere Variable. Die Energiefunktion beschreibt dabei Energiespeicherungsvorgänge aufgrund der Thermoelastizität, der Engergiedifferenz zwischen den beiden betrachteten Phasen sowie die Energiespeicherung infolge der Entwicklung von Inneren Spannungen. Das geschichtsabhängige Materialverhalten von Formgedächtnislegierungen wird dagegen mit Hilfe von Evolutionsgleichungen modelliert. Das System der Konstitutivgleichungen erfüllt den 2. Hauptsatz der Thermodynamik in Form der Clausius–Duhem Ungleichung.
A closed‐form analysis of material and geometry effects on stress singularities at unsymmetric bimaterial notches
Authors: Nachname Müller, A.; Hohe, J.; Becker, W. 
Published: April 2003

AbstractAn important issue in the mechanics of adhesive bonds is the knowledge of local mechanical fields. In the present study, an asymptotic analysis of the stress fields near an unsymmetric bimaterial notch with arbitrary opening angle is performed. Using the complex potential method, the order of the singularity of the stress fields at a notch tip can be determined in closed‐form analytical manner, so that the dependency of the occurring singularity exponents on geometry and material properties can be studied systematically.
Fractional calculus applied to radiation damping
Authors: Trinks C. , Ruge P. 
Published: April 2003

AbstractSeparate treatment of the low– and high–frequency part of the dynamic stiffness is essential when approximating the latter in the frequency–domain. In this paper, a doubly–asymptotic rational approximation of the low–frequency part is combined with an analytical interpretation of the asymptotic part leading to a system of fractional differential equations to represent the force–displacement relationship. Here, an analogy between fading memory of viscoelastic materials and radiation damping of unbounded domains is visible.
Experimentelle Untersuchung des mechanischen Verhaltens von astfreiem Fichtenholz in radialer und tangentialer Richtung des Stamms
Authors: Fleischmann M. , Helnwein P. , Eberhardsteiner J. 
Published: April 2003

AbstractBasierend auf einer Kleinserie einaxialer Druckversuche an astfreiem Fichtenholz wird in diesem Aufsatz ein viskoelastisch‐plastisches Materialmodell für Druckbeanspruchung in radialer Richtung vorgestellt. Weiters wird das beobachtete Verhalten in tangentialer Richtung erläutert.
Non‐smooth forced oscillations of a delaminated sandwich beam
Authors: Müller I. , Schmieg H. , Vielsack P. 
Published: April 2003

AbstractForced oscillations of delaminated sandwich structures are dominated by impacts. During motion the gap between the two adjacent parts of the structure opens and closes periodically. Each contact gives rise to an impact, which leads to energy loss. A 4 DOF beam model with concentrated masses allows discussion of principle on the influence of internal dissipation due to the impacts on the non‐linear system's response and the evolution of impacts near resonance points. Experimental investigations confirm the numerical results.
Choked flows in open capillary channel
Authors: Rosendahl U. , Ohlhoff A. , Dreyer M. , Rath H.J. 
Published: April 2003

AbstractA forced liquid flow through an open capillary channel with free liquid surfaces is investigated. Since the free surfaces can only withstand a certain difference between the liquid pressure and the ambient pressure the flow rate in the channel is limited. The maximum flow rate is achieved when the surfaces collapse. A theoretical approach is presented which shows that the limitation of flow rate occurs due to choking. This theory confirms the results of an experiment performed on board the sounding rocket TEXUS‐37 to measure the maximum stationary volume flux of a forced flow through an open capillary channel.
Re.ned Modeling of the pressure redistribution/scrambling under consistent consideration of scalar turbulence effects in turbulent combustion
Authors: Maltsev A. , Sadiki A. , Janicka J. 
Published: April 2003

AbstractIn practical turbulent flow problems of engineering importance the coupling between velocity and scalar turbulence along with the variable density plays a non negligible role. For computations using second moment closure approach, the pressure redistribution/scrambling is the most critical term to be modeled as well known. Almost all existing models consist in rescating models derived on a constant density basis in a density weighted form. With regard to turbulent premixed combustion in fact, the application of such models to a range of transient one‐dimensional and two‐dimensional premixed flames in the flamelet regime has been found to yield unsatisfactory results, see [1]. As pointed out by Sadiki [2], the use of the Favre method must be consistently considered as far as open thermodynamic systems are concerned. Furthermore, the need for maintaining certain invariance properties, physical and mathematical realizability conditions in formulating turbulence models is well accepted. Because turbulent processes are irreversible, these efforts demand a carefull consideration of thermodynamic concepts.Based on the results in [1] and following [2], this work aims to derive a physically consistent formulation of the pressure redistribution/scrambling term under consideration of the variable density. Considering the case of premixed flames, the thermochemistry is included by means of a single reactive scalar ‐ the reaction progress variable.The accuracy of the model extensions proposed is demonstrated by comparing the numerical results with experimental data in opposed jet premixed flame configuration.
Movement of drops on a solid surface due to a contact angle gradient
Authors: Zielke Paul Ch. , Shankar Subramanian R. , Szymczyk Janusz A. , McLaughlin John B. 
Published: April 2003

AbstractExperimental results on the motion of liquid drops on horizontal surfaces resulting from a contact angle gradient are presented. Silicon surfaces were modified using dodecyltrichlorosilane to generate the gradient. Water drops with initial diameters of 0.31 ‐ 0.5 mm (15 ‐ 65 nl) were placed on the surface, their movement videotaped, and subsequently analyzed. To characterize the gradient surface the static contact angle was measured along the surface.
Schadenserkennung mit genetischen Algorithmen
Authors: Engelhardt M. , Stavroulakis G.E. , Antes H. 
Published: April 2003

AbstractDas Erkennen und nachfolgend die Lokalisierung einer Schädigung eines Bauteils mittels gemessener charakteristischer Daten führt auf ein inverses Schädigungsidentifizierungsproblem, welches als Optimierungsaufgabe mittels statischer Untersuchungen formuliert und anschliessend numerisch gelöst wird. Als Anwendungsbeispiel wird eine quadratische Scheibe mit einer oder mehreren Schädigungen im elastostatischen Bereich betrachtet, wobei die Schädigungen als Risse bzw. Ellipsen modelliert werden. Die Erzeugung der Messdaten wird hier ausschliesslich numerisch durchgeführt. Als Optimierungsverfahren zur Identifizierung der Schädigung wird ein genetischer Algorithmus verwendet. Das mechanische Problem wird mit der Hilfe der Randelementmethode modelliert. Die numerische Untersuchung betrachtet den Einfluss verschiedener relevanter Parameter, unter anderem die Anzahl der Ellipsen, die Anzahl und die Genauigkeit der Messungen und die Parameter der genetischen Optimierung.