bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caveolin-1, a Determinant of the Fate of MCF-7 Breast Cancer Cells
Authors: Chai Tina , Yue Wei , Xu Peng , Gildea John , Felder Robin 
Published: January 2024

Background: The scaffolding protein, caveolin-1 (Cav-1), participates in multiple cellular functions including promotion of sodium excretion from the kidney. Loss of expression of Cav-1 is associated with tumorigenesis of various types of cancer. We have shown the potential link between hypertension and breast cancer via abnormal function of the G protein–coupled receptor kinase type 4 (GRK4). Objective: The current studies tested the hypothesis that Cav-1 acts as a tumor-suppressive factor in breast cancer cells and enhances the sensitivity to the inhibitory effect of the type 1 dopaminergic receptor (D1R). Methods: Michigan Cancer Foundation (MCF) MCF-7 cells stably expressing a Cav-1/mCherry fusion protein or mCherry alone were used as models to examine the effect of Cav-1 on cell growth, apoptosis, and senescence. Cell proliferation was determined by cell counting, cell cycle analysis (flow cytometry), and BrdU incorporation. Apoptosis was determined using the Cell Death Detection ELISA kit from Roche Diagnosis. Senescence was determined using the senescence associated beta galactosidase (SA-β-gal) assay. Reactive oxygen species (ROS) was measured using 2′,7′-dichlorodihydrofluorescein diacetate. Western blot analysis was used to measure activation of signaling pathway molecules. All statistical analyses were conducted with Microsoft Excel. Results: Overexpression of Cav-1 in MCF-7 cells reduced cellular growth rate. Both inhibition of proliferation and induction of cell death are contributing factors. Multiple signaling pathways were activated in Cav-1-expressing MCF-7 cells. Activation of Akt was prominent. In MCF-7-expressing Cav-1 (MCF-7 Cav-1) cells, the levels of phosphorylated Akt at S473 and T308 were increased 28- and 8.7-fold, respectively. Instead of protecting cells from apoptosis, extremely high levels of activated Akt resulted in increased levels of ROS which led to apoptosis and senescence. The tumor-suppressive effect plus downregulation of GRK4 makes Cav-1-expressing MCF-7 cells significantly more sensitive to the inhibitory effect of the D1R agonist, SKF38393. Conclusion: Caveolin-1 acts as a tumor-suppressing factor via extreme activation of Akt and down regulation of survival factors such as GRK4, survivin, and cyclin D1.
Microstructural Design and Mechanical Enhancement of δ-TRIP Steel Through Mo Micro-alloying and Isothermal Bainitic Transformation
Authors: Gholambargani Mahsa , Palizdar Yahya , Khanlarkhani Ali , Najafi Soroush 
Published: January 2024

This research was done to investigate third-generation Advanced High-Strength Steel (AHSS) to improve mechanical properties and reduce production costs in the automotive industry. The potential of TRIP-assisted steel has been studied to address this issue, with a special focus on δ-TRIP steels. This research studied the δ-TRIP samples, which were micro-alloyed with 0.05 and 0.30 wt.% Mo, with isothermal bainitic transformation (IBT) time parameters. Microstructural analyses were conducted using optical microscopy (OM), field emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectrometry (EDX). The volume percentage of retained austenite (RA) phase, critical to achieving the requisite mechanical characteristics, was determined using X-ray diffraction (XRD). The analysis of the samples revealed a complex, multi-phased structure comprising δ-ferrite matrix, α-ferrite, bainite, RA, and martensite. The quantity and morphology of RA significantly influenced the resulting mechanical characteristics. The experimental results demonstrated yield stresses (YS) of 400–440 MPa, ultimate tensile strength (UTS) of 790–870 MPa, and total elongations to failure (TEL) of 24–34% in δ-TRIP steels containing 0.05 and 0.30 wt.% Mo alloy. The optimal combination of UTS and TEL (UTS × TEL) is achieved at an impressive 850 MPa × 32% (27200 MPa.%). This remarkable achievement was made possible by adding only 0.30 wt.% Mo and IBT, which were carried out at 450°C for 10 min. Focusing on the effects of Mo-alloy content and heat treatment duration on microstructure and mechanical characteristics, this study provided insights into enhancing the quality of δ-TRIP steel while decreasing manufacturing costs.
Measuring Neuroplasticity in Response to Cardiovascular Exercise in People With Stroke: A Critical Perspective
Authors: De Las Heras Bernat , Rodrigues Lynden , Cristini Jacopo , Moncion Kevin , Ploughman Michelle , Tang Ada , Fung Joyce , Roig Marc 
Published: January 2024

Background Rehabilitative treatments that promote neuroplasticity are believed to improve recovery after stroke. Animal studies have shown that cardiovascular exercise (CE) promotes neuroplasticity but the effects of this intervention on the human brain and its implications for the functional recovery of patients remain unclear. The use of biomarkers has enabled the assessment of cellular and molecular events that occur in the central nervous system after brain injury. Some of these biomarkers have proven to be particularly valuable for the diagnosis of severity, prognosis of recovery, as well as for measuring the neuroplastic response to different treatments after stroke. Objectives To provide a critical analysis on the current evidence supporting the use of neurophysiological, neuroimaging, and blood biomarkers to assess the neuroplastic response to CE in individuals poststroke. Results Most biomarkers used are responsive to the effects of acute and chronic CE interventions, but the response appears to be variable and is not consistently associated with functional improvements. Small sample sizes, methodological variability, incomplete information regarding patient’s characteristics, inadequate standardization of training parameters, and lack of reporting of associations with functional outcomes preclude the quantification of the neuroplastic effects of CE poststroke using biomarkers. Conclusion Consensus on the optimal biomarkers to monitor the neuroplastic response to CE is currently lacking. By addressing critical methodological issues, future studies could advance our understanding of the use of biomarkers to measure the impact of CE on neuroplasticity and functional recovery in patients with stroke.
Accuracy and reliability of perception of bar velocity loss for autoregulation in resistance exercise
Authors: da Silva Drumond Gilo , da Silva Rodrigo Fabio Bezerra , Gantois Petrus , Nascimento Vitor Bertoli , Nakamura Fábio Yuzo , Fonseca Fabiano de Souza 
Published: January 2024

Background The perception of bar velocity loss (PVL) can be used as an alternative method for autoregulating intraset repetitions during velocity-based training. This study analyzed the accuracy and reliability of the PVL as a method to autoregulate the intraset repetitions using a moderate velocity loss (VL) threshold (15–30%). Methods A total of 22 resistance-trained men were familiarized with the PVL in a single session. Test–retest was performed in two sessions 1 week apart, in which participants completed three sets of bench press and back squat at 40, 60, and 80% 1 repetition maximum (1RM). Participants stopped the sets when they reached a moderate VL zone using their PVL. Accuracy was assessed by analyzing whether the mean VL (actual) and the lower and upper limits of the confidence interval (CI) (95%) of each set were within the established VL range and quantifying the relative frequency of correctly interrupted sets. Test–retest reliability was examined by the intraclass correlation coefficient and coefficient of variation. Results PVL showed acceptable accuracy in both exercises at 60 and 80% 1RM (50–65% success rate) and retest for all loads (53–76% success rate). Similar PVL percentages were observed between most of the sets for the test and retest ( p > 0.05). Accuracy improved during the retest, especially for bench press (40% 1RM) and back squat (40 and 80% 1RM). PVL was not reliable in the test–retest comparison. Conclusion PVL can be a strategy with acceptable accuracy to autoregulate the intraset repetitions in a moderate zone (VL15–30%), but its reliability does not appear satisfactory after one familiarization session.
Dying whilst on probation: a scoping review of mortality amongst those under community justice supervision
Authors: Perrett SE , Craddock C , Gray BJ 
Published: January 2024

Aim: Data suggest mortality rates of those under community justice services such as probation or parole have been increasing year on year. Little is known about why and how these individuals are dying. This scoping review explores the causes and contributing factors of mortality in those under community justice supervision. Methods: Studies published between 2011 and 2021 were identified across CINAHL, Embase, Global Health, Ovid Medline and PsycINFO. Articles were included if they presented original data on either mortality rates among those under community justice supervision or risk factors associated with the mortality of those under community justice supervision. Results: Searches identified 101 unique articles of which 13 were included in the review. Articles were representative of five countries. All articles were either retrospective reviews or retrospective cohort studies. The studies fell into the categories of all-cause mortality, self-inflicted deaths or drug-related deaths. Conclusion: Mortality rates of those under community justice supervision were found to be consistently higher than mortality rates for the general population regardless of cause of death. Factors identified as affecting mortality included history of drug use, history of self-harm and previous imprisonment including length of time in custody and experience of hospitalisation or solitary confinement while in custody.
Concurrent validity and test-retest reliability of Squegg™ - smart dynamometer and handgrip trainer in healthy individuals
Authors: Amin Zarmina , Gutierrez Galilea , True Larissa 
Published: January 2024

Introduction Handgrip strength is crucial for overall health and is often evaluated using hand dynamometers. Two hand dynamometers were used in the study: the Jamar® Hydraulic Hand Dynamometer and Squegg™ Smart Dynamometer and Handgrip Trainer, which is a relatively new, Bluetooth-enabled device to measure handgrip strength. The purpose of the study was to compare Jamar and Squegg to determine concurrent validity and, separately, test-retest reliability of Squegg. Methods The cross-sectional study measured bimanual handgrip strength using the Jamar and Squegg in two sessions, following standard protocols. Participants were 18+ years old, with no hand disease or dysfunction. Spearman’s rank correlations were used to evaluate concurrent validity between Jamar and Squegg. Test-retest reliability was evaluated with intraclass correlation coefficient (ICC) estimates and 95% confidence intervals calculated using a mean-rating ( k = 2) absolute agreement, 2-way mixed effects model. Results Data from 594 participants were used to evaluate concurrent validity. Moderately strong correlations between Jamar and Squegg values for the right hand (RH) (ρ = 0.670; p = .001) and Jamar and Squegg values for the left hand (LH) (ρ = 0.730; p = .001) were found, indicating ‘good’ concurrent validity. Test-retest reliability for Squegg ranged from good to excellent (RH, ICC = 0.911, 95% CI (0.895, 0.924), p = .001; LH, ICC = 0.928, 95% CI (0.915, 0.939), p = .001). Discussion The Squegg demonstrated good concurrent validity with Jamar and good-to-excellent test-retest reliability. It can be recommended as an alternative to the Jamar as a handgrip strength measurement tool. Squegg, which uses Bluetooth technology, is a reliable and cost-effective option for consistent handgrip strength testing.
Reimagine the ICU: Healthcare Professionals’ Perspectives on How Environments (Can) Promote Patient Well-Being
Authors: Kim Chan Mi , der Heide Esther M. van , van Rompay Thomas J. L. , Ludden Geke D. S. 
Published: January 2024

Objective: This study aims (1) to understand the needs and challenges of the current intensive care unit (ICU) environments in supporting patient well-being from the perspective of healthcare professionals (HCPs) and (2) to explore the new potential of ICU environments enabled by technology. Background: Evidence-based design has yielded how the design of environments can advocate for patient well-being, and digital technology offers new possibilities for indoor environments. However, the role of technology in facilitating ICU patient well-being has been unexplored. Method: This study was conducted in two phases. First, a mixed-method study was conducted with ICU HCPs from four Dutch hospitals. The study investigated the current environmental support for care activities, as well as the factors that positively and negatively contribute to patient experience. Next, a co-creation session was held involving HCPs and health technology experts to explore opportunities for technology to support ICU patient well-being. Results: The mixed-method study revealed nine negative and eight positive patient experience factors. HCPs perceived patient emotional care as most challenging due to the ICU workload and a lack of environmental support in fulfilling patient emotional needs. The co-creation session yielded nine technology-enabled solutions to address identified challenges. Finally, drawing from insights from both studies, four strategies were introduced that guide toward creating technology to provide holistic and personalized care for patients. Conclusion: Patient experience factors are intertwined, necessitating a multifactorial approach to support patient well-being. Viewing the ICU environment as a holistic unit, our findings provide guidance on creating healing environments using technology.
Splenic torsion in a cat with chronic anemia
Authors: Bucknoff Melissa C , Rolph Kerry E 
Published: January 2024

Case summary A 4-year-old female spayed domestic shorthair cat with chronic anemia was evaluated for acute-onset lethargy, vomiting, abdominal distension, and a palpably enlarged and firm spleen. Abdominal ultrasound confirmed marked splenomegaly and concern for a splenic infarct, prompting exploratory abdominal surgery, where splenic torsion was diagnosed. A splenectomy was performed, and the cat recovered uneventfully. Splenitis was diagnosed on histopathology. Anemia improved postoperatively. The role of chronic anemia and other concurrent findings in the development of splenic torsion in this case remains unknown. Relevance and novel information Splenic torsion has not been previously reported in cats, making this the first case of its kind. In cases of splenomegaly and abnormal splenic blood flow, splenic torsion should be considered a differential diagnosis in cats.
Salmonella enterica subspecies enterica serotype Typhimurium induced pyelonephritis and suspected multifocal myositis in a cat
Authors: Wilson Remon , Barrs Vanessa R 
Published: January 2024

Case summary A 2-year-old male neutered domestic shorthair cat presented with an acute onset of muscular pain, ataxia and fever. Serological tests for Toxoplasma gondii IgM and IgG, cryptococcal antigen, feline immune deficiency virus antibody and feline leukaemia virus antigen were all negative. Brain and spinal MRI showed evidence of myositis and bilateral renal parenchymal abnormalities and pyelectasis. Salmonella enterica subspecies enterica serotype Typhimurium 1,4, [5],12:i:1,2 was isolated from urine and was susceptible to amoxycillin, amoxycillin–clavulanic acid, enrofloxacin and trimethoprim–sulfonamide. All clinical signs resolved after a 2-week treatment course with oral amoxycillin–clavulanate. A repeat urine culture 7 days after completing the antimicrobial course was negative. Relevance and novel information Infection with Salmonella species is uncommon in cats and has not previously been reported in association with pyelonephritis or generalised myositis. The importance of performing urine culture in the initial diagnostic investigation of cats with pyrexia is highlighted in this case report.
The German version of the mHealth App Usability Questionnaire (GER-MAUQ): Translation and validation study in patients with cardiovascular disease
Authors: Tacke Theodora , Nohl-Deryk Pascal , Lingwal Neelam , Reimer Lara Marie , Starnecker Fabian , Güthlin Corina , Gerlach Ferdinand M , Schunkert Heribert , Jonas Stephan , Müller Angelina 
Published: January 2024

Objective In Germany, only a few standardized evaluation tools for assessing the usability of mobile Health apps exist so far. This study aimed to translate and validate the English patient version for standalone apps of the mHealth App Usability Questionnaire (MAUQ) into a German version. Methods Following scientific guidelines for translation and cross-cultural adaptation, the patient version for standalone apps was forward and back-translated from English into German by an expert panel. In total, 53 participants who were recruited as part of the beta testing process of the recently developed mHealth app HerzFit, answered the questions of the German version of the MAUQ (GER-MAUQ) and the System Usability Scale. Subsequently, a descriptive as well as a psychometric analysis was performed to test validity and reliability. Results After conducting three cognitive interviews, five items were modified. The values for Cronbach alpha for the entire questionnaire and the three subscales (0.966, 0.814, 0.910, and 0.909) indicate strong internal consistency. The correlation analysis revealed that the scores of the GER-MAUQ, the subscales and the SUS were strongly correlated with each other. The correlation coefficient of the SUS and the GER-MAUQ overall score was r = 0.854, P < 0.001 and the coefficients of the subscales and the SUS were r = 0.642, P < 0.001; r = 0.866, P < 0.001 and r = 0.643, P < 0.001. Conclusions We have developed a novel German version of the MAUQ and demonstrated it as a reliable and valid measurement tool for assessing the usability of standalone mHealth apps from the patients’ perspective. The GER-MAUQ allows a new form of standardized assessment of usability of mHealth apps for patients with cardiovascular disease in Germany. Further research with a larger sample and other samples is recommended.
Navigating the infodemic: A qualitative study of university students’ information strategies during the COVID-19 pandemic
Authors: Gies Lieve , Gogoi Mayuri , Bayliss Christopher D. , Pareek Manish , Webb Adam 
Published: January 2024

Objectives We aimed to study the strategies which university students developed for vetting information during the COVID-19 pandemic and associated infodemic. Methods We conducted semi-structured interviews with 34 students, using a piloted topic guide which explored several areas of pandemic experiences, including students’ use of media. Transcripts were analysed inductively following the thematic approach. Higher order themes were finalised following a coding exercise undertaken by two of the authors. Results Participants were acutely aware of misinformation during the pandemic. They rated legacy news media (print and broadcast media with pre-Internet origins) higher than social media for reliable information about the pandemic. However, strikingly, not all legacy media were automatically trusted and not all social media were uniformly distrusted. Participants identified a set of mechanisms for establishing whether a piece of information was truthful and accurate. These mechanisms had four main focal points: (1) the source, (2) the message, (3) individual media literacy and (4) the trustworthiness of others. Despite possessing a critical awareness of misinformation, participants avoided posting anything in relation to the pandemic for fear of becoming the target of online abuse. Conclusions In addition to underscoring the role of media literacy, our research foregrounds the need to attend to the importance of fostering media confidence. We define media confidence as the ability of digital media users to challenge and interrogate questionable or inaccurate information safe in the knowledge that there are adequate regulatory mechanisms in place to curb abuse, trolling and intimidation.
Real-Time Detection of Circulating Thrombi in an Extracorporeal Circuit Using Doppler Ultrasound: In-Vitro Proof of Concept Study
Authors: Peer Syed Murfad , Desai Manan , Bukhari Syed , Delores Yunchuan , Jonas Richard , Sinha Pranava 
Published: January 2024

Background Thromboembolic stroke continues to be by far the most common severe adverse event in patients supported with mechanical circulatory assist devices. Feasibility of using Doppler ultrasound to detect circulating thrombi in an extracorporeal circuit was investigated. Methods A mock extracorporeal circulatory loop of uncoated cardiopulmonary bypass tubing and a roller pump was setup. A Doppler bubble counter was used to monitor the mean ultrasound backscatter signal (MUBS). The study involved two sets of experiments. In Scenario 1, the circuit was sequentially primed with human blood components, and the MUBS was measured. In Scenario 2, the circuit was primed with heparinized fresh porcine blood, and the MUBS was measured. Fresh blood clots (diameter <1,000 microns, 1,000-5,000 microns, >5,000 microns) were injected into the circuit followed by protamine administration. Results In Scenario 1 (n = 3), human platelets produced a baseline MUBS of 1.5 to 3.5 volts/s. Addition of packed human red blood cells increased the baseline backscatter to 17 to 21 volts/s. Addition of fresh frozen plasma did not change the baseline backscatter. In Scenario 2 (n = 5), the blood-primed circuit produced a steady baseline MUBS. Injection of the clots resulted in abrupt and transient increase (range: 3-30 volts/s) of the baseline MUBS. Protamine administration resulted in a sustained increase of MUBS followed by circuit thrombosis. Conclusions Doppler ultrasound may be used for real-time detection of circulating solid microemboli in the extracorporeal circuit. This technology could potentially be used to design safety systems that can reduce the risk of thromboembolic stroke associated with mechanical circulatory support therapy.