bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictive value of serum albumin and calcium levels in burn patients with Pseudomonas aeruginosa infection: A comprehensive analysis of clinical outcomes
Authors: Haghighi Bardineh Seyed Ashkan , Balou Heydar Ali , Sedigh Ebrahim‐Saraie Hadi , Mobayen Mohammadreza , Esmailzadeh Mojdeh , Haghighi Samane , Haghighi Arezoo , Sadeghi Mahsa 
Published: February 2024

AbstractIn the ongoing challenge to reduce burn‐associated mortality rates, this study explores the predictive capacity of clinical factors in burn patients, focusing on vitamin D, calcium, and serum albumin levels during hospitalisation in cases with Pseudomonas aeruginosa infection. Our research involves a comprehensive analysis of 100 burn patients, encompassing crucial clinical parameters such as the burn severity index, serum albumin, vitamin D, and calcium levels at admission. Data were meticulously entered into IBM Statistics SPSS software version 28 and subjected to statistical analysis. The study reveals an average patient age of 39.75 years and a notable 34% mortality rate. Additionally, the average lengths of hospital and intensive care unit (ICU) stays are determined to be 11.33 and 7.79 days, respectively. Significantly, a correlation between calcium and albumin variables and treatment outcomes is established, showcasing their potential to predict variable changes in patient mortality rates. Furthermore, a noteworthy association is observed between serum calcium levels and the duration of ICU hospitalisation. In conclusion, albumin and calcium variables emerge as sensitive and specific indicators for predicting outcomes in burn patients. Importantly, the independence of these factors from the physician's experience and diagnosis reduces human error and thus increases the accuracy of mortality prediction in this patient population.
Association of nurse managers' paternalistic leadership and nurses' perceived workplace bullying: The mediating effect of organizational climate
Authors: Peng Xiao , Zeng Qingsong , Yang Dongliang , Cheng Yuanjuan , Zhao Shengxiu , Song Jinping , Qin Yuelan , Gao Zumei , Chen Yuan , Zhang Fengjian , Huang Lei , Mo Beirong , Liu Yilan 
Published: February 2024

AbstractAimsTo explore the association between nurse managers' paternalistic leadership and nurses' perceived workplace bullying (WPB), as well as to examine the mediating role of organizational climate in this association.BackgroundThere is a lack of empirical evidence regarding the relationship between nurse managers' paternalistic leadership, organizational climate and nurses' perceived WPB. Clarifying this relationship is crucial to understand how paternalistic leadership influences WPB and for nursing managers to seek organizational‐level solutions to prevent it.MethodsA cross‐sectional survey was performed from 4 January to 10 February 2022, in six tertiary hospitals in mainland China. Demographic information, Paternalistic Leadership Scale, Organizational Climate Scale and Negative Acts Questionnaire‐Revised were used in the survey. Descriptive statistics, Spearman correlation analyses and a structural equation model were used for data analysis.ResultsA total of 5093 valid questionnaires were collected. Moral leadership and authoritarian leadership have both direct and indirect effects on WPB through the mediating effect of organizational climate. The former is negatively related to WPB and the latter is positively related to WPB. Benevolent leadership was only negatively associated with WPB via the mediating effect of organizational climate.ConclusionThe three components of paternalistic leadership have different effects on WPB through the mediating effect of organizational climate. Nurse managers are recommended to strengthen moral leadership, balance benevolent leadership, reduce authoritarian leadership and strive to create a positive organizational climate in their efforts to mitigate WPB among nurses.ImpactThis study enhanced our comprehension of the relationship between different leadership styles and WPB. Greater emphasis should be placed on moral leadership in the promotion of nursing managers and nursing leadership training programs. Additionally, nursing managers should focus on establishing a positive organizational climate that helps to reduce WPB.Patient or Public ContributionNo patient or public contribution. This study did not involve patients, service users, caregivers or members of the public.
The association between parents' ethnic socialization and positive and negative interethnic contact among majority and minority groups in China and the moderating role of essentialism
Authors: Huang Fei , Shamloo Soraya E. , Li Linpeng , Cocco Veronica M. , Vezzali Loris 
Published: February 2024

AbstractIn a multiethnic country like China, ethnic membership is an important dimension of social construction, and interethnic contact is a necessary component of social interactions. Family is the context where ethnic socialization takes place and where parents play a relevant role. The present study focused on the Hui‐Han interethnic context. Measures of perceived parent's ethnic socialization, interethnic contact, and essentialism were administrated to Hui minority (N = 560) and Han majority (N = 954) secondary students. Results indicated that parents' positive ethnic socialization (cultural socialization/pluralism, promotion of harmony) was associated with greater positive and lower negative contact, while negative ethnic socialization (preparation for bias, promotion of mistrust) had opposite effects. Essentialist views of ethnicity moderated the associations of perceived parents' positive ethnic socialization with positive contact: the association between positive ethnic socialization and positive contact was stronger among individuals with lower (vs. higher) essentialist views. Results did not differ across the majority and the minority group. Implications for prompting positive interethnic interactions and preventing negative contact are discussed.
Milestone review: GABA, from chemistry, conformations, ionotropic receptors, modulators, epilepsy, flavonoids, and stress to neuro‐nutraceuticals
Authors: Johnston Graham A. R. , Beart Philip M. 
Published: February 2024

AbstractArising out of a PhD project more than 50 years ago to synthesise analogues of the neurotransmitter GABA, a series of new chemical entities were found to have selective actions on ionotropic GABA receptors. Several of these neurochemicals are now commercially available. A new subtype of these receptors was discovered that could be a target for the treatment of myopia, the facilitation of learning and memory, and the improvement of post‐stroke motor recovery. The development of these new chemical entities over many years demonstrates the importance of neurochemicals with which to investigate selective aspects of GABA receptors and illustrates the significance of collaboration between chemists and biologists in neurochemistry. Vital were the improvements in synthetic organic chemistry and the use of functional human receptors expressed in oocytes. Current interest in ionotropic GABA receptors includes the clinical development of subtype‐specific agents and the role of gain‐of‐function receptor variants in epilepsy. Dietary flavonoids were found to cross the blood–brain barrier to influence brain function. Natural and synthetic flavonoids had a range of effects on GABA receptors, ranging from positive, silent, and negative allosteric modulators, to even second‐order modulation of first‐order modulators. Flavonoids have been called “a new family of benzodiazepines.” Like benzodiazepines, flavonoids reduce stress. Stress produces changes in GABA receptors in the brain that may be because of changes in endogenous modulators, such as neurosteroids and corticosteroids. GABA also occurs naturally in the diet leading to studies of the effects of oral GABA on brain function. This finding has resulted in studies of GABA and related neurochemicals as neuro‐nutraceuticals. GABA systems in the gut microbiome are essential to such studies. The actions of oral GABA and of GABA‐enriched beverages and foodstuffs are now an area of considerable scientific and commercial interest. GABA is a deceptively simple chemical that can take up many shapes, which may underlie its complex functions. The need for new chemical entities with selective actions for further studies highlights the need for continuing collaboration between chemists and biologists.image
School Connectedness and Suicide Among High School Youth: A Systematic Review
Authors: Welty Cody W. , Bingham Lindsay , Morales Mario , Gerald Lynn B. , Ellingson Katherine D. , Haynes Patricia L. 
Published: February 2024

ABSTRACTBACKGROUNDSuicide is a leading cause of death for adolescents, and school connectedness is a potential, modifiable protective factor for suicide. We sought to examine if school connectedness protected against suicide among high school students and if potential moderators affected the relationship between school connectedness and suicide.METHODSWe searched online databases (PubMed, EMBASE, CINAHL, and PsycINFO) on December 12, 2021, for studies that examined the effects of school connectedness on suicide among high school students.RESULTSThis systematic review identified 34 studies that examined the effects of school connectedness on adolescent suicidality. Results indicated mixed findings of school connectedness on suicidality. Among studies that assessed a suicide ideation outcome, 73.3% found that school connectedness protected against suicide. Among studies that assessed a suicide attempts outcome, 50% found that school connectedness protected against suicide. Most included studies did not control for notable variables in their final models, such as sleep, impulsivity, substance use, or depression. No studies examined moderators of school connectedness and suicide.CONCLUSIONSSchool connectedness is somewhat protective of suicidality, and more protective of suicidal ideation than suicide attempts. Researchers should examine the construct of school connectedness among modern youth to better understand school connectedness and suicide.
Oral Lactobacillus zeae exacerbates the pathological manifestation of periodontitis in a mouse model
Authors: Chen Yi‐Wen , Hou Yu‐Wen , Wang Chuang‐Wei , Cheng Shih‐Jung , Kuo Wei‐Ting , Lin Chun‐Pin , Hou Hsin‐Han 
Published: February 2024

AbstractIntroductionThe worldwide prevalence of periodontitis is considerably high, and its pathogenic mechanisms must be investigated and understood in order to improve clinical treatment outcomes and reduce the disease prevalence and burden. The exacerbation of the host immune system induced by oral microbial dysbiosis and the subsequent tissue destruction are the hallmarks of the periodontitis. However, the oral bacteria involved in periodontitis are not fully understood. We used the Oxford Nanopore Technologies (ONT) sequencing system to analyze metagenomic information in subgingival dental plaque from periodontitis and non‐periodontitis patients. The number of Lactobacillus zeae (L. zeae) in the periodontitis patients was 17.55‐fold higher than in the non‐periodontitis patients, suggesting that L. zeae is a novel periodontitis‐associated pathogen. Although several Lactobacillus species are used in vivo as probiotics to treat periodontitis and compete with Porphyromonas gingivalis (P. gingivalis), the roles of L. zeae in periodontitis progression, and the relationship between L. zeae and P. gingivalis needs to be investigated.MethodsBoth L. zeae and P. gingivalis were inoculated in the ligature‐implant site of periodontitis mice. We collected mouse gingival crevicular fluid to analyze inflammatory cytokine secretion using a multiplex assay. Intact or sliced mouse maxilla tissue was used for micro‐computed tomography analysis or hematoxylin and eosin staining, immunohistochemistry, and tartrate‐resistant acid phosphatase staining to evaluate alveolar bone loss, neutrophil infiltration, and osteoclast activation, respectively.ResultsWe observed that L. zeae competed with P. gingivalis, and it increased inflammatory cytokine secretion at the ligature‐implant site. Similar to P. gingivalis, L. zeae promoted ligature‐induced neutrophile infiltration, osteoclast activation, and alveolar bone loss.DiscussionWe, therefore, concluded that L. zeae accelerated the progression of periodontitis in the ligature‐induced periodontitis mouse model.
Whether Patients with Anterior Cruciate Ligament Reconstruction Walking at a Fast Speed Show more Kinematic Asymmetries?
Authors: Lai Huahao , Chen Xiaoling , Huang Wenhan , Xie Zhenyan , Yan Yuan , Kang Ming , Zhang Yu , Huang Jiehua , Zeng Xiaolong 
Published: February 2024

ObjectiveKnee kinematic asymmetries after anterior cruciate ligament reconstruction (ACLR) are correlated with poor clinical outcomes, such as the progression of knee cartilage degenerations or reinjuries. Fast walking in patients with knee conditions may exacerbate knee kinematic asymmetries, but its impact on ACLR patients is uncertain. The aim of this study is to investigate if fast walking induces more knee kinematic asymmetries in unilateral ACLR patients.MethodsThis cross‐sectional study enrolled 55 patients with unilateral ACLR from January 2020 to July 2022. There were 48 males and seven females with an average age of 30.6 ± 6.4 years. Knee kinematic data were collected at three walking speeds: self‐selected, fast (150% normal), and slow (50% normal). A 3D knee kinematic analysis system measured the data, and self‐reported outcomes assessed comfort levels during walking. We used SPM1D for two‐way repeated ANOVA and posthoc paired t‐tests to analyze kinematic differences in groups.ResultsIn fast walking, ACLR knees exhibited more transverse kinematic asymmetries than intact knees, including greater external rotation angle (1.8°, 38%–43%; gait cycle [GC], p < 0.05 & 1.8–2.7°, 50%–61% GC, p < 0.05) and increased proximal tibial translation (2.1–2.5 mm, 2%–6% GC, p < 0.05 & 2.5–3.2 mm, 92%–96% GC, p < 0.05). Additionally, ACLR knees showed greater posterior tibial translation than intact knees (3.6–3.7 mm, 7%–8% GC, p < 0.05) during fast walking. No posterior tibial translation asymmetries were observed in slow walking compared to normal walking levels. ACLR knees have the most comfortable feelings in slow walking speed, and the most uncomfortable feelings in fast walking speed levels (29%).ConclusionsFast walking induces additional external tibial rotation and proximal and posterior tibial translation asymmetries in ACLR patients. This raises concerns about long‐term safety and health during fast walking. Fast walking, not self‐selected speed, is beneficial for identifying postoperative gait asymmetries in ACLR patients.
Supply and Demand Balance Control Strategy for Microgrids Considering Uncertainty and Disturbance Based on Online H∞ Policy Iteration
Authors: Liu Yang , Jiang Zhanpeng , Xing Zuoxia , Liu Hengyu , Li Pengtao , Han Xingfan 
Published: February 2024

Abstract
With the rapid growth of renewable energy generation, its proportion in power generation is increasing. Accompanied by this, due to the uncertain characteristics of renewable energy generation, the issue of imbalanced power supply and demand in microgrids has posed, which brings great challenges to the reliability of microgrid. In recent years, how to use an energy storage system to solve this problem has become a hot research topic in microgrid. In this paper, an online H∞ policy iteration algorithm is proposed to control the output power of photovoltaic arrays and micro-gas turbines. At the same time, considering the uncertainty of the load demand in the microgrid system and the input disturbance of the micro-gas turbine governor, the power supply and demand relationship of the microgrid can be balanced by the proposed control strategy. Finally, the effectiveness and practicality of the control strategy were demonstrated through simulation analysis.
Database of all currently known mobile colistin resistance genes
Authors: Rebelo Ana Rita , Hendriksen Rene S. 
Published: February 2024

ABSTRACT

We created a database of all currently known mobile colistin resistance genes and variants (
n
= 115). It contains accession numbers of the gene and protein sequences, mutations between the protein variants and the main proteins, and additional metadata. It is accompanied by all genetic and protein sequences as two aggregated FASTA files.
Complete genome sequence of new
Microbacterium
sp. strain ET2, isolated from roots of leafless orchid
Authors: Volynchikova Elena A. , Khrenova Maria G. , Panova Tatiana V. , Rodin Vladimir A. , Zvereva Maria I. , Tsavkelova Elena A. 
Published: February 2024

ABSTRACT

Whole-genome sequence of ET2 strain, isolated from the roots of leafless orchid, constitutes a single circular chromosome of 3,604,840 bp (69.44% G + C content). BLAST+-based average nucleotide identity (ANIb) and digital DNA-DNA hybridization values indicate that ET2 may be a novel
Microbacterium
species. Genes putatively involved in plant-microbial interactions were predicted.
Draft genome sequence of
Methylobacterium
sp. OT2 isolated from human skin and capable of growing on
t
-octylphenol polyethoxylate
Authors: Son Haelim , Huang Shir-Ly , Lee Kyoung 
Published: February 2024

ABSTRACT

Here, we present the draft whole-genome sequence of
Methylobacterium
sp. OT2, isolated from human skin on a minimal medium containing
t
-octylphenol ethoxylate (Triton X-100). This genomic information contributes to understanding the niche adaptation on human skin and its catabolism of Triton X-100 in this strain.
Complete genome sequence of
Vibrio
sp. strain AH4, a close relative of
Vibrio metoecus
isolated from Nile tilapia (
Oreochromis niloticus
)
Authors: Al-Harbi Ahmed H. 
Published: February 2024

ABSTRACT

Here, I report the complete genome sequence of
Vibrio
sp. strain AH4, which had been isolated from moribund farmed Nile tilapia (
Oreochromis niloticus
). Assessment of the genome sequence of this strain revealed the presence of two linear chromosomes 2,894,109 bp and 1,082,372 bp.
Complete genome sequences of StopSmel and Aussie, two Mu-like bacteriophages of
Sinorhizobium meliloti
Authors: Nielander Macy , Maybank Mya , Massimino Crissy , Fitzgerald John , Blossum Hannah , Douthitt Cayce , Holland Chris , Hunter Wayne B. , Carrol Megan , D'Elia Tom 
Published: February 2024

ABSTRACT

We report the complete genome sequences of two bacteriophages, Aussie and StopSmel, isolated from soil using the host
Sinorhizobium meliloti
NRRL L-50. The genomes are similar in length and gene content and share 76% nucleotide identity. Comparative analysis of Aussie and StopSmel identified core functional modules associated with Mu-like bacteriophages.
Pathogenic and genomic characterization of rabbit-sourced
Pasteurella multocida
serogroup F isolates recovered from dead rabbits with respiratory disease
Authors: Wang Jinxiang , Sun Shikun , Chen Dongjin , Gao Chenfang , Sang Lei , Xie Xiping 
Published: February 2024

ABSTRACT

Pasteurella multocida
serogroup F can infect a number of animals. However, the pathogenicity and genomic features of this serogroup are still largely unknown. In the present study, the pathogenicity and genomic sequences of 19 rabbit-sourced
P. multocida
serogroup F isolates were determined. The 19 isolates were highly pathogenic for rabbits causing severe pathologic lesions and high mortality in inoculated rabbits. Nevertheless, the pathologic lesions in rabbits caused by the 19 isolates were distinct from those caused by the previously reported high-virulent serogroup F strains J-4103 (rabbit), P-4218 (turkey), and C21724H3km7 (chicken). Moreover, the 19 isolates were avirulent to white feather broilers. The genomes of the 19 isolates were determined to understand the pathogenicity of these isolates. The finding of a number of functional genes in the 19 isolates by comparison with the low-virulent rabbit-sourced serogroup F strain s4 might contribute to the high virulence of these isolates. Notably, polymorphisms were determined in the lipopolysaccharide outer core biosynthetic genes
natC
and
gatF
among the serogroup F strains of different hosts. However, the sequences of
natC
and
gatF
from rabbit-sourced strains (except for SD11) were identical, which might be responsible for the host specific of the 19 isolates. The observations and findings in this study would be helpful for the understanding of the pathogenicity variation and host predilection of
P. multocida
.

IMPORTANCE

The 19 rabbit-sourced
Pasteurella multocida
serogroup F isolates showing high virulence to rabbits were avirulent to the broilers. Notably, polymorphisms were determined in the lipopolysaccharide outer core biosynthetic genes
natC
and
gatF
among all serogroup F strains of different hosts. However, the sequences of
natC
and
gatF
from rabbit-sourced strains (except for SD11) were identical, which might be responsible for the host specific of the 19 isolates.
Resensitizing tigecycline- and colistin-resistant
Escherichia coli
using an engineered conjugative CRISPR/Cas9 system
Authors: Zhang Haijie , Chen Bo , Wang Zeyu , Peng Kai , Liu Yuan , Wang Zhiqiang 
Published: February 2024

ABSTRACT

Tigecycline and colistin were referred to as the “last resort” antibiotics in defending against carbapenem-resistant, Gram-negative bacterial infections, and are currently widely used in clinical treatment. However, the emergence and prevalence of plasmid-mediated
tet
(X4) and
mcr-1
genes pose a serious threat to the therapeutic application of tigecycline and colistin, respectively. In this research, a tigecycline- and colistin-resistant bacteria resensitization system was developed based on efficient and specific DNA damage caused by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Associated Protein 9 (Cas9) nucleases. A conjugation method was used to deliver the resensitization system, which harbors two single-guide RNAs targeting
tet
(X4) and
mcr-1
genes and constitutively expressed Cas9. The conjugation efficiency was nearly 100% after conjugation condition optimization
in vitro
, and the resensitivity efficiency for clinical isolates was over 90%. In addition, when performing resensitization
in vivo
, the resistance marker was replaced with a glutamate-based, chromosomal, plasmid-balanced lethal system to prevent the introduction of additional resistance genes in clinical settings, making this strategy a therapeutic approach to combat the
in vivo
spread of antibiotic resistance genes (ARGs) among bacterial pathogens. As a proof of concept, this resensitive system can significantly decrease the counts of tigecycline- and colistin-resistant bacteria to 1%
in vivo
. Our study demonstrates the efficacy and adaptability of CRISPR-Cas systems as powerful and programmable antimicrobials in resensitizing
tet
(X4)- and
mcr-1-
mediated, tigecycline- and colistin-resistant strains, and opens up new pathways for the development of CRISPR-based tools for selective bacterial pathogen elimination and precise microbiome composition change.

IMPORTANCE

The emergence of plasmid-encoded
tet
(X4) and
mcr-1
isolated from human and animal sources has affected the treatment of tigecycline and colistin, and has posed a significant threat to public health. Tigecycline and colistin are considered as the “last line of defense” for the treatment of multidrug-resistant (MDR) Gram-negative bacterial infections, so there is an urgent need to find a method that can resensitize
tet
(X4)-mediated tigecycline-resistant and
mcr-1
-mediated colistin-resistant bacteria. In this study, we developed a glutamate-based, chromosomal, plasmid-balanced lethal conjugative CRISPR/Cas9 system, which can simultaneously resensitize
tet
(X4)-mediated tigecycline-resistant and
mcr-1
-mediated colistin-resistant
Escherichia coli
. The counts of tigecycline- and colistin-resistant bacteria decreased to 1%
in vivo
after the resensitization system was administered. This study opens up new pathways for the development of CRISPR-based tools for selective bacterial pathogen elimination and precise microbiome composition change.