bluuebunny/crossref_metadata_2025_split huggingface dataset:
CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana
Authors: Kim Gyung‐Tae , Fujioka Shozo , Kozuka Toshiaki , Tax Frans E. , Takatsuto Suguru , Yoshida Shigeo , Tsukaya Hirokazu 
Published: February 2005

SummaryBrassinosteroids (BRs) are plant hormones that are essential for a wide range of developmental processes in plants. Many of the genes responsible for the early reactions in the biosynthesis of BRs have recently been identified. However, several genes for enzymes that catalyze late steps in the biosynthesis pathways of BRs remain to be identified, and only a few genes responsible for the reactions that produce bioactive BRs have been identified. We found that the ROTUNDIFOLIA3 (ROT3) gene, encoding the enzyme CYP90C1, which was specifically involved in the regulation of leaf length in Arabidopsis thaliana, was required for the late steps in the BR biosynthesis pathway. ROT3 appears to be required for the conversion of typhasterol to castasterone, an activation step in the BR pathway. We also analyzed the gene most closely related to ROT3, CYP90D1, and found that double mutants for ROT3 and CYP90D1 had a severe dwarf phenotype, whereas cyp90d1 single knockout mutants did not. BR profiling in these mutants revealed that CYP90D1 was also involved in BR biosynthesis pathways. ROT3 and CYP90D1 were expressed differentially in leaves of A. thaliana, and the mutants for these two genes differed in their defects in elongation of hypocotyls under light conditions. The expression of CYP90D1 was strongly induced in leaf petioles in the dark. The results of the present study provide evidence that the two cytochrome P450s, CYP90C1 and CYP90D1, play distinct roles in organ‐specific environmental regulation of the biosynthesis of BRs.
The transcript composition of egg cells changes significantly following fertilization in wheat (Triticum aestivum L.)
Authors: Sprunck Stefanie , Baumann Ute , Edwards Keith , Langridge Peter , Dresselhaus Thomas 
Published: February 2005

SummaryHere, we report the transcript profile of wheat egg cells and proembryos, just after the first cell division. Microdissected female gametophytes of wheat were used to isolate eggs and two‐celled proembryos to construct cell type‐specific cDNA libraries. In total, 1197 expressed sequence tags (ESTs) were generated. Analysis of these ESTs revealed numerous novel transcripts. In egg cells, 17.6% of the clustered ESTs represented novel transcripts, while 11.4% novel clusters were identified in the two‐celled proembryo. Functional classification of sequences with similarity to previously characterized proteins indicates that the unfertilized egg cell has a higher metabolic activity and protein turnover than previously thought. Transcript composition of two‐celled proembryos was significantly distinct from egg cells, reflecting DNA replication as well as high transcriptional and translational activity. Several novel transcripts of the egg cell are specific for this cell. In contrast, some fertilization induced novel mRNAs are abundant also in sporophytic tissues indicating a more general role in plant growth and development. The potential functions of genes based on similarity to known genes involved in developmental processes are discussed. Our analysis has identified numerous genes with potential roles in embryo sac function such as signaling, fertilization or induction of embryogenesis.
Cap‐independent translation of maize Hsp101
Authors: Dinkova Tzvetanka D. , Zepeda Hilda , Martínez‐Salas Encarnación , Martínez Luz María , Nieto‐Sotelo Jorge , de Jiménez Estela Sánchez 
Published: February 2005

SummaryMaize embryonic axes contain stored mRNAs, some of which are able to undergo cap‐independent translation initiation during germination. The Hsp101 mRNA, encoding a heat shock protein, is essential for thermo‐tolerance induction and is present among the stored transcripts. This research aimed to investigate whether the Hsp101 transcript is IRES‐driven regulated upon heat stress. Hsp101 transcribed either in vitro or in vivo was efficiently translated via a cap‐independent mechanism. This was observed either in an animal in vitro translation system containing proteolytically cleaved eukaryotic initiation factor eIF4G or in a plant system lacking both eIF4E and eIFiso4E initiation factors. Deletion of the 5′ untranslated region (UTR) from the Hsp101 mRNA abolished its cap‐independent translation indicating that this nucleotide sequence is required to confer cap‐independent initiation. Bicistronic constructs containing the Hsp101 mRNA 5′UTR in sense and anti‐sense directions between two reporter genes were translated in both cap‐independent systems. A similar bicistronic construct containing a viral internal ribosome entry site (IRES) element between the reporter genes was used as control. Internal translation of the second reporter gene was observed when the Hsp101 5′UTR was in the sense but not in the anti‐sense orientation in the bicistronic construct. Taken together, these data suggest that the 5′UTR of maize Hsp101, a plant cellular mRNA, functions as an IRES‐like element accounting for its cap‐independent translation during heat stress.
Modeling Family‐Centered Pediatric Nursing Care: Strategies for Shift Report
Authors: Ryan Eileen , Steinmiller Elizabeth 
Published: February 2005

ISSUES AND PURPOSE. Family‐centered care philosophies place the family as central to the plan of care. These philosophies are often challenging for nurses to put into clinical practice. The purpose of this article is to provide interventions for nurses to use with families who are stressed.CONCLUSIONS. Families' coping styles may be subject to judgments from the nurses, and these judgments may be passed on during shift report. Labels that are passed on in report can influence nurses' care.PRACTICE IMPLICATIONS. This article describes positive communication strategies for shift report that can facilitate successful family‐centered care.
Impact of new prostheses on the oral health related quality of life of edentulous patients
Authors: Veyrune J. L. , Tubert‐Jeannin S. , Dutheil C. , Riordan P. J. 
Published: February 2005

Objective:  A study was conducted to evaluate the impact of the placement of complete dentures by using the Global Oral Health Assessment Index (GOHAI).Background:  Oral health quality of life indicators can be used to evaluate the effects of dental treatments.Material and methods:  The 26 participants were treated in a French University Clinic during 2002. They were randomly divided into two groups. Each group received new prostheses, but evaluation of the quality of life was made at different periods [baseline, denture placement (group 1), 6 and 12 weeks (group 2) after placement]. A questionnaire was used to collect information on patient's satisfaction with the previous and new prostheses. Nonparametric tests were used to test the relationships between patients’ satisfaction or baseline data and GOHAI variations with time as well as to compare mean values of GOHAI within each group.Results:  At baseline, the impact of oral health problems was apparent; the mean GOHAI‐Add score was 45.8 (10.2). Six weeks after placement of the new denture, there was no difference in GOHAI scores compared with the initial assessment. An improvement in GOHAI score was observed 12 weeks after the participants received their new dentures (p < 0.05). Change in GOHAI‐Add scores was negatively correlated with the initial GOHAI‐Add score. Patients who preferred the new prosthesis enjoyed a positive change in GOHAI scores (p < 0.001). There was a relationship between participants’ satisfaction with the new dentures and change in GOHAI scores (p < 0.05).Conclusion:  The GOHAI can be used to evaluate needs for and effect of the making of new complete dentures.
The masticatory handicap of wearing removable dentures in elderly men
Authors: Liedberg Birgitta , Stoltze Kaj , Öwall Bengt 
Published: February 2005

Objective:  This study aimed at describing the masticatory handicap of wearing removable dentures measured by some masticatory tests and the intake of hard and soft foods.Materials and methods:  The subjects were participants in a comprehensive health examination of 67–68‐year‐old men living in Malmö, Sweden. Four hundred eighty‐three men took part in a clinical oral examination which recorded the number of teeth and removable dentures. One hundred and one had complete dentures in both jaws and 131 had removable partial dentures in different combinations. Masticatory tests used almonds to record the number of stokes to the first swallow, and two‐coloured chewing gums for recording bolus kneading and shaping. A nutritionist paid a home visit to assess dietary habits including the consumption of hard and soft foods. The data could be split into groups of different tooth and denture situations which were large enough to enable statistical analysis to be carried out. The results of a regression analysis of the group with different numbers of natural teeth (NT) and no removable dentures could be used as a reference for correlation with the masticatory capacity of removable denture wearers.Results:  Number of strokes to the first swallow revealed no masticatory limitations of wearing removable dentures, while chewing gum colour‐mixing and shaping revealed more differentiated impairments equivalent to the function of five to 16 teeth in a remaining natural dentition. Hard food intake for the removable denture groups was comparable to 17–19 NT. Soft food intake was not influenced by denture wearing. In a ranking of oral conditions, those with more than 24 NT had the highest test values for all tests, and those with complete sets of dentures the lowest except for the number of strokes to the first swallow of an almond.Conclusion:  Removable denture wearing can be regarded as a handicap when measured with objective masticatory tests using chewing gum and the intake of hard foods. The number of chewing strokes to the first swallow of an almond and the intake of soft foods is not affected by the wearing of removable dentures.
Understanding Intentions in Social Interaction: The Role of the Anterior Paracingulate Cortex
Authors: Walter Henrik , Adenzato Mauro , Ciaramidaro Angela , Enrici Ivan , Pia Lorenzo , Bara Bruno G. 
Published: February 2005

Abstract
Neuroimaging studies have identified the anterior paracingulate cortex (PCC) as the key prefrontal region subserving theory of mind. We adopt an evolutionary perspective hypothesizing that, in response to the pressures of social complexity, a mechanism for manipulating information concerning social interaction has emerged in the anterior PCC. To date, neuroimaging studies have not properly distinguished between intentions of persons involved in social interactions and intentions of an isolated person. In two separate fMRI experiments, we demonstrated that the anterior PCC is not necessarily involved in the understanding of other people's intentions per se, but primarily in the understanding of the intentions of people involved in social interaction. Moreover, this brain region showed activation when a represented intention implies social interaction and therefore had not yet actually occurred. This result suggests that the anterior PCC is also involved in our ability to predict future intentional social interaction, based on an isolated agent's behavior. We conclude that distinct areas of the neural system underlying theory of mind are specialized in processing distinct classes of social stimuli.
Frontal and Temporo-Parietal Lobe Contributions to Theory of Mind: Neuropsychological Evidence from a False-Belief Task with Reduced Language and Executive Demands
Authors: Apperly Ian A. , Samson Dana , Chiavarino Claudia , Humphreys Glyn W. 
Published: February 2005

Abstract
A model of the functional and anatomical basis of belief reasoning is essential for understanding the relationship between belief reasoning and other cognitive processes in both normal development and pathology. Studies of brain-damaged patients can give valuable insights into the nature of belief processing but pose unique methodological problems. The current study addresses these problems by using a nonlinguistic belief-reasoning task with substantially reduced executive demands. A case series of 12 brain-damaged patients is presented. The belief-reasoning errors of four patients with damage to the prefrontal cortex appeared to arise from these patients' executive function problems. The belief-reasoning errors of three patients with damage to the temporo-parietal junction could not easily be accounted for in this way, raising the possibility that this brain region has a necessary role in representing beliefs, rather than handling the executive demands of belief-reasoning tasks. We discuss the importance of gaining empirical evidence about the scope of “theory of mind” impairments, and the important role for neuropsychological studies in this project.
Observations of Drizzle in Nocturnal Marine Stratocumulus
Authors: vanZanten M. C. , Stevens B. , Vali G. , Lenschow D. H. 
Published: February 2005

Abstract
In situ and radar data from the second field study of the Dynamics and Chemistry of Marine Stratocumulus (DYCOMS-II) have been used to study drizzle in stratocumulus. Measurements indicate that drizzle is prevalent. During five of seven analyzed flights precipitation was evident at the surface, and on roughly a third of the flights mean surface rates approached or exceeded 0.5 mm day−1. Additional analysis of the structure and variability of drizzle indicates that the macroscopic (flight averaged) mean drizzle rates at cloud base scale with H3/N where H is the flight-averaged cloud depth and N the flight-averaged cloud droplet number concentration. To a lesser extent flight-to-flight variability in the mean drizzle rate also scales well with differences in the 11- and 4-μm brightness temperatures, and the cloud-top effective radius. The structure of stratocumulus boundary layers with precipitation reaching the surface is also investigated, and a general picture emerges of large flight-averaged drizzle rates being manifested primarily through the emergence of intense pockets of precipitation. The characteristics of the drizzle spectrum in precipitating versus nonprecipitating regions of a particular cloud layer were mostly distinguished by the number of drizzle drops present, rather than a change in size of the median drizzle drop, or the breadth of the drizzle spectrum.
Inferring Fall Attitudes of Pristine Dendritic Crystals from Polarimetric Radar Data
Authors: Matrosov Sergey Y. , Reinking Roger F. , Djalalova Irina V. 
Published: February 2005

Abstract
Single pristine planar ice crystals exhibit some flutter around their preferential horizontal orientation as they fall. This study presents estimates of flutter and analyzes predominant fall attitudes of pristine dendritic crystals observed with a polarization agile Ka-band cloud radar. The observations were made in weakly precipitating winter clouds on slopes of Mt. Washington, New Hampshire. The radar is capable of measuring the linear depolarization ratios in the standard horizontal–vertical polarization basis (HLDR) and the slant 45°–135° polarization basis (SLDR). Both HLDR and SLDR depend on crystal shape. HLDR also exhibits a strong dependence on crystal orientation, while SLDR depends only weakly on orientation. The different sensitivities of SLDR and HLDR to the shape and orientation effects are interpreted to estimate the angular flutter of crystals. A simple analytical expression is derived for the standard deviation of angular flutter as a function of the HLDR to SLDR ratio assuming perfect radar system characteristics. The flutter is also assessed by matching theoretical and observed depolarization patterns as a function of the elevation of the radar’s beam. The matching procedure is generally more robust since it accounts for the actual polarization states and imperfections in the radar hardware. The depolarization approach was used to estimate flutter of falling pristine dendrites that were characterized by Reynolds numbers in a range of approximately 40–100. Using the matching approach, this flutter was found to be about 9° ± 3°, as expressed by the standard deviation of the crystal minor axes from the vertical direction. The analytical expression provides a value of flutter of about 12°, which is at the high end of the estimate obtained by the matching procedure. The difference is explained by the imperfections in the polarization states and radar hardware, so the analytical result serves as an upper bound to the more robust result from matching. The values of flutter estimated from the experimental example are comparable to estimates for planar crystals obtained in laboratory models and by individual crystal sampling.
Zonal Asymmetries, Teleconnections, and Annular Patterns in a GCM
Authors: Cash Benjamin A. , Kushner Paul J. , Vallis Geoffrey K. 
Published: February 2005

Abstract
The influence of zonally asymmetric boundary conditions on the leading modes of variability in a suite of atmospheric general circulation models is investigated. The set of experiments consists of nine model configurations, with varying degrees and types of zonal asymmetry in their boundary conditions. The structure of the leading EOF varies with the zonal asymmetry of the base state for each model configuration. In particular, a close relationship is found between the structure of the EOF and the model storm tracks. An approximately linear relationship is found to hold between the magnitude of the zonal asymmetry of the leading EOF and of the storm tracks in the models. It is shown that this linear relationship extends to the observations.
One-point correlation maps centered on the regions where the EOFs reach their maximum amplitude show similar structures for all configurations. These structures consist of a north–south dipole, resembling the observed structure of the North Atlantic Oscillation (NAO). They are significantly more zonally localized than the leading EOF, but do resemble one-point correlation maps and sector EOFs calculated for a simulation with zonally symmetric boundary conditions. Thus, the leading EOF for each simulation appears to represent the longitudinal distribution of zonally localized NAO-like patterns. This longitudinal distribution appears to be tied to the distribution of high-frequency eddies, as represented by the storm tracks. A detailed momentum budget for each case confirms that high-frequency eddies play a crucial role in producing the NAO-like patterns. Other dynamical processes also play an important role, but vary with the details of the simulation.
An Arctic Springtime Mixed-Phase Cloudy Boundary Layer Observed during SHEBA
Authors: Zuidema P. , Baker B. , Han Y. , Intrieri J. , Key J. , Lawson P. , Matrosov S. , Shupe M. , Stone R. , Uttal T. 
Published: February 2005

Abstract
The microphysical characteristics, radiative impact, and life cycle of a long-lived, surface-based mixed-layer, mixed-phase cloud with an average temperature of approximately −20°C are presented and discussed. The cloud was observed during the Surface Heat Budget of the Arctic experiment (SHEBA) from 1 to 10 May 1998. Vertically resolved properties of the liquid and ice phases are retrieved using surface-based remote sensors, utilize the adiabatic assumption for the liquid component, and are aided by and validated with aircraft measurements from 4 and 7 May. The cloud radar ice microphysical retrievals, originally developed for all-ice clouds, compare well with aircraft measurements despite the presence of much greater liquid water contents than ice water contents. The retrieved time-mean liquid cloud optical depth of 10.1 ± 7.8 far surpasses the mean ice cloud optical depth of 0.2, so that the liquid phase is primarily responsible for the cloud’s radiative (flux) impact. The ice phase, in turn, regulates the overall cloud optical depth through two mechanisms: sedimentation from a thin upper ice cloud, and a local ice production mechanism with a time scale of a few hours, thought to reflect a preferred freezing of the larger liquid drops. The liquid water paths replenish within half a day or less after their uptake by ice, attesting to strong water vapor fluxes. Deeper boundary layer depths and higher cloud optical depths coincide with large-scale rising motion at 850 hPa, but the synoptic activity is also associated with upper-level ice clouds. Interestingly, the local ice formation mechanism appears to be more active when the large-scale subsidence rate implies increased cloud-top entrainment. Strong cloud-top radiative cooling rates promote cloud longevity when the cloud is optically thick. The radiative impact of the cloud upon the surface is significant: a time-mean positive net cloud forcing of 41 W m−2 with a diurnal amplitude of ∼20 W m−2. This is primarily because a high surface reflectance (0.86) reduces the solar cooling influence. The net cloud forcing is primarily sensitive to cloud optical depth for the low-optical-depth cloudy columns and to the surface reflectance for the high-optical-depth cloudy columns. Any projected increase in the springtime cloud optical depth at this location (76°N, 165°W) is not expected to significantly alter the surface radiation budget, because clouds were almost always present, and almost 60% of the cloudy columns had optical depths &gt;6.
Biomechanical Analysis of a Combined Double-Bundle Posterior Cruciate Ligament and Posterolateral Corner Reconstruction
Authors: Sekiya Jon K. , Haemmerle Marcus J. , Stabile Kathryne J. , Vogrin Tracy M. , Harner Christopher D. 
Published: February 2005

Background Failure to address both components of a combined posterior cruciate ligament and posterolateral corner injury has been implicated as a reason for abnormal biomechanics and inferior clinical results. Hypothesis Combined double-bundle posterior cruciate ligament and posterolateral corner reconstruction restores the kinematics and in situ forces of the intact knee ligaments. Study Design Controlled laboratory study Methods Ten fresh-frozen human cadaveric knees were tested using a robotic testing system through sequential cutting and reconstructing of the posterior cruciate ligament and posterolateral corner. The knees were subjected to a 134-N posterior tibial load and a 5-N.m external tibial torque at multiple flexion angles. The double-bundle posterior cruciate ligament reconstruction was performed using Achilles and semitendinosus tendons. The posterolateral corner reconstruction consisted of reattaching the popliteus tendon to its femoral origin and reconstructing the popliteofibular ligament with a gracilis tendon. Results Under the posterior load, the combined reconstruction reduced posterior translation to within 1.2 - 1.5 mm of the intact knee. The in situ forces in the posterior cruciate ligament grafts were significantly less than those in the native posterior cruciate ligament at all angles except full extension. Conversely, the forces in the posterolateral corner grafts were significantly higher than those in the native structures at all angles. Under the external torque with the combined reconstruction, external rotation as well as in situ forces in the posterior cruciate ligament and posterolateral corner grafts were not different from the intact knee. Conclusions A combined posterior cruciate ligament and posterolateral corner reconstruction can restore intact knee kinematics at time zero. In situ forces in the intact posterior cruciate ligament and posterolateral corner were not reproduced by the reconstruction; however, the posterolateral corner reconstruction reduced the loads experienced by the posterior cruciate ligament grafts. Clinical Relevance By addressing both structures of this combined injury, this technique restores native kinematics under the applied loads at fixed flexion angles and demonstrates load sharing among the grafts creating a potentially protective effect against early failure of the posterior cruciate ligament grafts but with increased force in the posterolateral corner construct.