bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colorectal cancer with low SLC35A3 is associated with immune infiltrates and poor prognosis
Authors: Lu Shuai , Sun Xibo , Tang Huazhen , Yu Jinxuan , Wang Bing , Xiao Ruixue , Qu Jinxiu , Sun Fang , Deng Zhuoya , Li Cong , Yang Penghui , Yang Zhenpeng , Rao Benqiang 
Published: January 2024

AbstractThe expression level of SLC35A3 is associated with the prognosis of many cancers, but its role in colorectal cancer (CRC) is unclear. The purpose of our study was to elucidate the role of SLC35A3 in CRC. The expression levels of SLC35A3 in CRC were evaluated through tumor immune resource assessment (TIMER), The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), International Cancer Genome Consortium (ICGC), Human Protein Atlas (HPA), qRT-PCR, and immunohistochemical evaluation. TCGA, GEO, and ICGC databases were used to analyze the diagnostic and prognostic value of SLC35A3 in CRC. A overall survival (OS) model was constructed and validated based on the expression level of SLC35A3 and multivariable analysis results. The cBioPortal tool was used to analyze SLC35A3 mutation in CRC. The UALCAN tool was used to analyze the promoter methylation level of SLC35A3 in colorectal cancer. In addition, the role of SLC35A3 in CRC was determined through GO analysis, KEGG analysis, gene set enrichment analysis (GSEA), immune infiltration analysis, and immune checkpoint correlation analysis. In vitro experiments validated the function of SLC35A3 in colorectal cancer cells. Compared with adjacent normal tissues and colonic epithelial cells, the expression of SLC35A3 was decreased in CRC tissues and CRC cell lines. Low expression of SLC35A3 was associated with N stage, pathological stage, and lymphatic infiltration, and it was unfavorable for OS, disease-specific survival (DSS), recurrence-free survival (RFS), and post-progression survival (PPS). According to the Receiver Operating Characteristic (ROC) analysis, SLC35A3 is a potential important diagnostic biomarker for CRC patients. The nomograph based on the expression level of SLC35A3 showed a better predictive model for OS than single prognostic factors and TNM staging. SLC35A3 has multiple types of mutations in CRC, and its promoter methylation level is significantly decreased. GO and KEGG analysis indicated that SLC35A3 may be involved in transmembrane transport protein activity, cell communication, and interaction with neurotransmitter receptors. GSEA revealed that SLC35A3 may be involved in energy metabolism, DNA repair, and cancer pathways. In addition, SLC35A3 was closely related to immune cell infiltration and immune checkpoint expression. Immunohistochemistry confirmed the positive correlation between SLC35A3 and helper T cell infiltration. In vitro experiments showed that overexpression of SLC35A3 inhibited the proliferation and invasion capability of colorectal cancer cells and promoted apoptosis. The results of this study indicate that decreased expression of SLC35A3 is closely associated with poor prognosis and immune cell infiltration in colorectal cancer, and it can serve as a promising independent prognostic biomarker and potential therapeutic target.
Sophocarpine alleviates doxorubicin-induced heart injury by suppressing oxidative stress and apoptosis
Authors: Zhang Hong-jin , Fu Yang , Zhang Huang , Lai Ze-qun , Dong Yi-Fei 
Published: January 2024

AbstractDoxorubicin (DOX) is an effective anti-tumor drug accompanied with many side effects, especially heart injury. To explore what effects of sophocarpine (SOP) on DOX-induced heart injury, this study conducted in vivo experiment and in vitro experiment, and the C57BL/6J mice and the H9C2 cells were used. The experimental methods used included echocardiography, enzyme-linked immunosorbent assay (ELISA), dihydroethidium (DHE) staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, western blotting and so on. Echocardiography showed that SOP alleviated DOX-induced cardiac dysfunction, as evidenced by the improvements of left ventricle ejection fraction and left ventricle fractional shortening. DOX caused upregulations of creatine kinase (CK), creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH), while SOP reduced these indices. The relevant stainings showed that SOP reversed the increases of total superoxide level induced by DOX. DOX also contribute to a higher level of MDA and lower levels of SOD and GSH, but these changes were suppressed by SOP. DOX increased the pro-oxidative protein level of NOX-4 while decreased the anti-oxidative protein level of SOD-2, but SOP reversed these effects. In addition, this study further discovered that SOP inhibited the decreases of Nrf2 and HO-1 levels induced by DOX. The TUNEL staining revealed that SOP reduced the high degree of apoptosis induced by DOX. Besides, pro-apoptosis proteins like Bax, cleaved-caspase-3 and cytochrome-c upregulated while anti-apoptosis protein like Bcl-2 downregulated when challenged by DOX, but them were suppressed by SOP. These findings suggested that SOP could alleviate DOX-induced heart injury by suppressing oxidative stress and apoptosis, with molecular mechanism activating of the Nrf2/HO-1 signaling pathway.
The amount of antioxidants in honey has a strong relationship with the plants selected by honey bees
Authors: Shakoori Zahra , Salaseh Elham , Mehrabian Ahmad Reza , Tehrani Dariush Minai , Dardashti Niluofar Famil , Salmanpour Farid 
Published: January 2024

AbstractAs one of the main sources of natural antioxidants, flowering plants play a role in the prevention and treatment of many diseases directly and indirectly. Honey is considered as an important nutrient in the supply of natural antioxidants, the amount of which is directly dependent on the plant origin and geographical location of the bee feeding place. The existence of valuable communities of native and endemic plant species has turned Alborz, Zagros and Azerbaijan into the most important hubs of honey production in Iran. In this study, we collected samples of honey from more than 90 regions in Alborz, Zagros and Azerbaijan during the years 2020 to 2021. We evaluated the samples using melissopalynology method and measuring the amount of antioxidant activity. The rise of antioxidant activity in honey is dependent on the abundance of some plant families as well botanical origins. The abundance of plant families Rosaceae, Amaranthaceae, Fabaceae and Asteraceae showed a higher influence on the amount of antioxidants in honey than other plant families. Also, the abundance of plant families Rosaceae and Fabaceae increased with increasing altitude. In general, the amount of antioxidant activity of honey samples shows a different percentage under the influence of ecological and geographical changes.
18F-FDG PET/CT features of Meigs syndrome induced by ovarian sex cord stromal tumors: a retrospective clinical study
Authors: Hu Xianwen , Li Wenxin , Li Xiaotian , Li Dandan , Cai Jiong , Wang Pan 
Published: January 2024

AbstractThe objective of this study was retrospectively to analyze the clinical characteristics and 18F-FDG PET/CT findings in Meigs syndrome (MS) patients. A total of 21 patients with MS induced by ovarian stromal tumors and 69 patients with pseudo-MS caused by ovarian cancer (OC-PMS) were subjected to evaluation using 18F-FDG PET/CT. Visual and semi-quantitative methods were employed to analyze the PET/CT findings. Visual analysis included recording whether the density of the primary tumor was uniform, whether there were cystic changes and calcifications, and the location of serous fluid accumulation. Semi-quantitative analysis involved the measurement of the tumor size, SUVmax, and SUVmean. No significant difference was observed in the size and density of primary tumors between the MS group and the OC-PMS group. However, the SUVmax and SUVmean of tumors in the MS group were found to be significantly lower than those in the OC-PMS group. The amount of serous cavity effusion caused by ovarian sex cord stromal tumors was found to be unrelated to the size of the tumor, SUVmax, and SUVmean but was positively correlated with the level of Ca125. MS patients have both benign ovarian tumors and ascites and/or pleural effusion, which may be accompanied by elevated Ca125 levels. This should be considered as one of the differential diagnoses for ovarian cancer. Understanding the PET/CT features of MS can facilitate the attainment of an accurate diagnosis before surgery.
Research on prevention and control technology of classified rockburst in TBM construction of deeply buried tunnels
Authors: Yang Yalei , Du Lijie , Li Qingwei , Gong Cheng , Song Yin , Wang Minyuan 
Published: January 2024

AbstractRock blasting and other geological disasters occur frequently in the TBM construction of deeply buried tunnels and seriously threaten construction safety and progress. Therefore, it is extremely important to conduct scientific research for effective prevention and control of rockbursts in construction. Based on a large number of field rockburst data, this study analyses the influence of rockburst on construction safety and efficiency by using statistical theory and summarizes the temporal and spatial characteristics of rockburst time, location and influence range. Using these results, combined with the characteristics of the TBM structure and construction method, classification prevention and control objectives, theoretical criteria and prevention and control technology of rock bursts are proposed. A theoretical system of classified prevention and control of rockburst is constructed, which is cooperatively controlled by microseismic monitoring, TBM equipment, TBM excavation and support measures. The system is verified to provide practical protection, demonstrating that this report provides an important reference for the prevention and control of rock bursts in ultradeep tunnels.
In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis
Authors: Nicoll Callum R. , Alvigini Laura , Gottinger Andrea , Cecchini Domiziana , Mannucci Barbara , Corana Federica , Mascotti María Laura , Mattevi Andrea 
Published: January 2024

AbstractMetabolons are protein assemblies that perform a series of reactions in a metabolic pathway. However, the general importance and aptitude of metabolons for enzyme catalysis remain poorly understood. In animals, biosynthesis of coenzyme Q is currently attributed to ten different proteins, with COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 forming the iconic COQ metabolon. Yet several reaction steps conducted by the metabolon remain enigmatic. To elucidate the prerequisites for animal coenzyme Q biosynthesis, we sought to construct the entire metabolon in vitro. Here we show that this approach, rooted in ancestral sequence reconstruction, reveals the enzymes responsible for the uncharacterized steps and captures the biosynthetic pathway in vitro. We demonstrate that COQ8, a kinase, increases and streamlines coenzyme Q production. Our findings provide crucial insight into how biocatalytic efficiency is regulated and enhanced by these biosynthetic engines in the context of the cell.
Regional and global hotspots of arsenic contamination of topsoil identified by deep learning
Authors: Wu Mengting , Qi Chongchong , Derrible Sybil , Choi Yosoon , Fourie Andy , Ok Yong Sik 
Published: January 2024

AbstractTopsoil arsenic (As) contamination threatens the ecological environment and human health. However, traditional methods for As identification rely on on-site sampling and chemical analysis, which are cumbersome, time-consuming, and costly. Here we developed a method combining visible near infrared spectra and deep learning to predict topsoil As content. We showed that the optimum fully connected neural network model had high robustness and generalization (R-Square values of 0.688 and 0.692 on the validation and testing sets). Using the model, the relative As content at regional and global scales were estimated and the human populations that might potentially be affected were determined. We found that China, Brazil, and California are topsoil As-contamination hotspots. Other areas, e.g., Gabon, although also at great risk, are rarely documented, making them potential hotspots. Our results provided guidance for regions that require more detailed detection or timely soil remediation and can assist in alleviating global topsoil-As contamination.
Highly effective and selective FeBr3-promoted deuterium bromination/cyclization of 1,n-enynes
Authors: Li Ping , Du Zhongjian , Wu Baofeng , Zhao Xin , You Yang'en 
Published: January 2024

A highly effective and selective FeBr3-promoted deuterium bromination/cyclization of 1,n-enynes has been developed, affording heterocyclic alkenyl brominative compounds with good results.
Opportunities and threats of cryosphere change to the achievement of UN 2030 SDGs
Authors: Wang Shijin 
Published: January 2024

AbstractThe cryosphere plays a critical role in maintaining the stability of the social-ecological system, but rapid cryosphere changes have been and are wide-ranging and have a profound affect, even threatening the achievement of the UN’s 2030 sustainable development goals (SDGs). In the study, we review the opportunities and threats caused by cryosphere changes in achieving the SDGs. The results reveal that cryosphere changes are significantly related to the supply of sustainable fresh water (SDG 6), alpine hydropower (SDG 7), and climate action (SDG 13). In addition, they favorably support life on land and below water (SDG 14-15), and effectively affect the livelihoods (SDG 1-5), agricultural development (SDG 2), snow/ice tourism (SDG 8), infrastructure (SDG 9), regional inequality (SDG 10), and cities and communities (SDG 11), as well as affecting Arctic shipping routes (SDG 16). Long-term cryosphere threats far outweigh their contributions to the SDGs. The cryosphere contributes little to human emissions, but it is significantly affected by climate change. Areas affected by cryosphere changes need to strengthen resilience and enhance the ability to adapt to the influences of cryosphere changes (SDG 1-17) via financial transfer, multilateral international cooperation, and other practical policies.
Spanish influenza 1918/19
Authors: Stopfner Maria 
Published: January 2024

Abstract
The study focuses on the Spanish influenza pandemic of 1918/19, tracing the crisis coverage of two
daily newspapers from two different countries, Austria and the United States, to reveal the general dynamics and
manipulative potential of mediatized public discourse in times of crisis. The pragma-rhetoric analysis examines,
first, which social actors gain access to the media to voice their opinion and, second, how they attempt to manipulate
public perception to avoid blame, for example, by instrumentalizing certain groups as scapegoats. The results of the
cross-national comparison of the two historic newspapers reveal patterns in crisis reporting on the Spanish flu that
are intriguingly reminiscent of contemporary media coverage of the recent Corona Pandemic.
Manipulating citizens’ beliefs and emotions
Authors: Ilie Cornelia 
Published: January 2024

Abstract
In times of crisis, such as the Covid-19 pandemic, social divisions caused by dissensus about
controversial government policies escalate due to widespread uncertainty and anxiety, fuelled by the proliferation of
covert and overt manipulative strategies in official discourses. Like citizens in other countries, Swedes received
during the Covid-19 crisis scarce or inconsistent information, and potentially misleading messages, while seeking to
preserve a trust-based and tradition-rooted national consensus. Using a pragma-rhetorical and argumentation framework
of analysis, this study scrutinizes the controversies emerging from the polarization of online comments made by
reactive and counter-reactive citizens who either supported or challenged the controversial policies promoted by the
Swedish Public Health Agency. The fallacies underlying the commenters’ arguments and counter-arguments show how
initially reasonable discussions turned into deeply polarized disputes, as a manifestation of social dissent.
How can governments be prevented from manipulating statistics about Covid-19?
Authors: Marinho Cristina , Billig Michael 
Published: January 2024

Abstract
This chapter is part of a project examining how politicians manipulate statistics and how they can
be institutionally countered. We rhetorically analyse how the Chair of the United Kingdom Statistics Authority
countered the Minister of Health who was manipulating the statistics for Covid-19 testing. The Chair wrote two public
letters to the Minister. The first was diplomatically phrased. When this letter failed to achieve its purpose, the
Chair wrote a more directly critical letter, which was effective. We analyse the letters and how they were reported in
the UK national press. The diplomatic letter was virtually ignored, but the second letter was widely reported, with
newspapers framing the story as a respected independent ‘watchdog’ censuring a minister for misusing statistics.
Lessons learned?
Authors: Breeze Ruth 
Published: January 2024

Abstract
The 2008 financial crisis severely undermined public trust in banking systems, prompting calls for
widespread reform. However, irregularities continued, leading to a further succession of scandals over the following
years. We should therefore ask how banks justified the catastrophe and their failure to reform. This chapter examines
two large UK-based banks: Royal Bank of Scotland (RBS), and HSBC. All the “letters to shareholders” published in their
Annual Reports over 15 years are examined for commonplace arguments or “topoi” offered as justifications for their
failure to comply with ethical standards. Five typical recurring arguments are analysed, with a particular focus on
their use in subtle manipulations of public opinion to draw attention away from the scale and impact of corporate
wrongdoing.
Linguistic change in the 20th century
Authors: Salameh Jiménez Shima 
Published: January 2024

Abstract
This paper analyses the colloquialization of football-match broadcastings in Peninsular Spanish throughout the 20th
century, in line with other recent works dealing with the colloquialization of mass-media. To do so, it accounts for (a)
linguistic features, (b) register variation, and (c) external factors related to colloquialization. The analysis is based on a
self-compiled database of audio-visual recordings aired between 1968 and the 2000s in Tv and radio. According to results,
colloquialization in Peninsular Spanish football broadcasts was developed during three main periods: 1960s–1980s, 1980s–1990s,
and 1990s up to now. Specifically, colloquialization can be measured by speech speed, vocabulary and the use of address terms,
among other features. These features, in turn, are associated with register variation properties, such as changes in the
proximity shared by speakers or the presence of day-to-day topics in broadcastings. Last, some external factors, such as the
influence of historical events or the role of announcers also seem to affect the results analysed.
French j’imagine, Spanish me imagino
Authors: Schneider Stefan 
Published: January 2024

Abstract
French imaginer and Spanish imaginar start to appear during the second half of the
13th century. Initially, these verbs remain closely linked to the corresponding nouns image and
imagen, their prevailing meaning being ‘form a picture’. After the 14th century, a second meaning,
‘suppose’, gradually begins to develop. The spread of the ‘suppose’ meaning favors, among other things, the appearance of
detached constructs, that is, deverbal discourse markers (Schneider
2020a, 2022), in the first person present indicative singular. From the
17th century to the 19th century, in these detached constructs, French imaginer is regularly preceded by a
subject pronoun and a reflexive pronoun, whereas Spanish imaginar occurs without pronouns. In the 20th
century and beginning 21st century, the French deverbal discourse marker without reflexive almost completely supplants the one
with reflexive. In contrast, the recent development of imaginar in Spanish goes in the opposite direction,
with me imagino replacing imagino as deverbal discourse marker. Hence, for centuries, French
and Spanish maintained a specific contrast regarding the discourse marker construction with imaginer and
imaginar: presence of the reflexive pronoun in French, absence of the reflexive pronoun in Spanish. In
the course of the 20th century, this contrast seems to have reversed. The present study seeks to provide additional data both
from written and spoken corpora and also addresses the question of whether the absence or, respectively, presence of the
reflexive pronoun in the discourse marker construction represents a merely superficial and formal phenomenon or is due to a
deeper structural and semantic difference.
Applying multimodal analysis
Authors: Weninger Csilla , Lim Fei Victor , Chen Yixiong 
Published: January 2024

Abstract
This chapter builds on Chapter 4 and introduces two main
applications within applied linguistics stemming from a
systemic-functional semiotic theory of multimodality: embodied
teaching and language textbook analysis. The chapter first gives
a brief overview of the broader field of research that each of
these applications is located in. Then each focal area is
elaborated and illustrated via a case study conducted by the
authors. Each case study provides a rationale for why multimodal
analysis is appropriate given the research focus and questions,
demonstrates how multimodal analysis was implemented and
conducted, and reflects on the challenges of its implementation
in applied linguistic research contexts.
Grounded Theory
Authors: Hadley Gregory , Hadley Hiromi 
Published: January 2024

Abstract
This chapter explores the underutilized
Grounded Theory Methodology (GTM) in applied linguistics,
detailing its procedures, objectives, benefits, and limitations
from a post-positivist perspective. It suggests ways for
educators and scholars to apply GTM to generate insightful
social interaction theories within classrooms and discusses the
role of technology in data collection. The text addresses
critiques and ethical questions associated with GTM. When implemented rigorously, GTM can enhance not
only teaching practices within applied linguistics but also
offer fresh insights to scholarly communities beyond this
field.
Applications of Grounded Theory in the field of Extensive
Reading
Authors: Hadley Gregory , Hadley Hiromi 
Published: January 2024

Abstract
This chapter discusses the application of
Extensive Reading (ER), an English language teaching method
encouraging learners to consume large amounts of
proficiency-level content, and its spread to second language
classrooms globally since the 1990s. Studies show that ER
significantly enhances learners’ vocabulary, reading speeds, and
language comprehension. Despite challenges of passive resistance
and apathy from learners, teachers from Japan, Italy, and
Southeast Asia are seeking ways to integrate ER into their
Academic English curricula. The chapter introduces a grounded
theory about learners’ social processes in the ER classroom,
explaining why the Grounded Theory Methodology (GTM) was
selected for this study, its implementation, the faced
challenges, addressed solutions, and unique insights gleaned
through GTM.