bluuebunny/crossref_metadata_2025_split huggingface dataset:
A trend analysis of Black American women with cardiovascular disease and chronic medical conditions, sociodemographic factors from NHANES From 2011 to 2020
Authors: Ripon Rezaul Karim , Rimti Fahmida Hoque , Kercy Mickelder , Hossain Shahriar , Motahara Umma , Hossain Md. Sharif , Sikder Md. Tajuddin 
Published: December 2023

Background
Significant racial and gender differences exist in the prevalence of CVD in the United States. The goal of this study is to evaluate the prevalence of CVD among Black American women, the relationship between CVD and some medical conditions, and significant sociodemographic factors.

Methods
The researchers in this study used data from four cycles of the NHANES, carried out by the NCHS. 2011 to 2012, 2013 to 2014, 2015 to 2016, and 2017 to 2020 were the cycles that were chosen. The researchers used the survey package in the R programming language to examine the data.

Results
People with CVD problems 20 years of age and older were included in the analyses. Black American women experienced a considerable prevalence of CVD from 2011 to 2020. These women were more likely to report having completed no more schooling than the ninth grade, being widowed/divorced/separated, and having undergone a hysterectomy, as well as having a history of diabetes, asthma, obesity, arthritis, and depression. Black American women with CVD had a 3.8-fold increased risk of diabetes and a 5.6-fold increased risk of arthritis.

Conclusion
This study shows that Black American women with CVD are more likely to have chronic illnesses such as hysterectomy, diabetes, asthma, obesity, arthritis, and depression. Black American women’s cardiovascular risk profiles can be updated using the data from this study.
Caracterização das reuniões de Equipe da Atenção Primária em um município da região Sul de Santa Catarina
Authors: Monteiro Bettiol Leticia , Zaneripe de Souza Nunes Rafael , Dos Santos Toczek Elizabeth , Bauer Torriani Marcos , Tuon Lisiane 
Published: December 2023

Introdução: A construção do trabalho com equipe multiprofissional, transforma a forma de se fazer saúde e produzir cuidados, através da prática integrativa e inovadora, porém, para que isso ocorra de fato, são necessárias novas formas de mobilização do coletivo, que ultrapassem, o saber de forma isolada. Objetivo: Descrever os processos de trabalho em relação às reuniões de equipe de um Município do Extremo Sul Catarinense. Métodos: Trata-se de um estudo guarda-chuva, transversal, quantitativo e de natureza descritiva, realizado nas unidades de um Município de médio porte localizado na mesorregião Sul de Santa Catarina, março de 2022. Resultados: Os resultados evidenciam que as reuniões de equipe ocorrem para grande maioria de forma semanal, trazendo assuntos como a organização do processo de trabalho, planejamento de ações e discussões de caso, onde a maior parte relatou ter de forma parcial, ou não ter o apoio da gestão municipal para o planejamento do processo de trabalho. Conclusão: As reuniões de equipe não apenas melhoram a eficácia dos cuidados de saúde, mas também fortalecem a base de nosso sistema de atenção primária à saúde. Elas desempenham um papel central na promoção de uma abordagem centrada no paciente.
Ujaran Kebencian di Media Sosial: Ditinjau dari Kematangan Emosi Dengan Kecerdasan Moral sebagai Mediator
Authors: Fathoni Cahyono Aryo Bima , Khalisah Ariella , Safitri Lily , Lestari Tri , Hudaya Yanuary Nurul 
Published: December 2023

Cases of hate speech that occur in Indonesia continue to increase over time. Seeing this phenomenon, this study aims to explore hate speech that occurs on social media in terms of emotional maturity with moral intelligence as a mediator. This research was conducted using quantitative methods. The scales used in this study were the scale of emotional maturity, moral intelligence, and vignette of hate speech. The number of participants in this study is as many as 268 undergraduate students aged 17-25 years. The results of the analysis show that moral intelligence can mediate the influence of emotional maturity on hate speech. These results provide a theoretical implication that emotional intelligence influences moral intelligence where this variable plays a strong role in hate speech. Kasus ujaran kebencian yang terjadi di Indonesia terus meningkat seiring berjalannya waktu. Fenomena tersebut membuat peneliti tertarik untuk melakukan studi dan mengetahui lebih jauh mengenai ujaran kebencian yang terjadi di media sosial ditinjau dari kematangan emosi dengan kecerdasan moral sebagai mediator. Metode yang digunakan dalam penelitian ini merupakan metode kuantitatif. Skala yang digunakan adalah skala kematangan emosi, kecerdasan moral, dan vignette ujaran kebencian. Jumlah partisipan pada penelitian ini sebanyak 268 mahasiswa strata-1 berusia 17 - 25 tahun. Dari hasil analisa, didapatkan bahwa kecerdasan moral dapat memediatori pengaruh kematangan emosi terhadap ujaran kebencian. Hasil tersebut memberikan implikasi teoretis bahwa kecerdasan emosi memberi pengaruh pada kecerdasan moral di mana variabel ini memainkan peran yang kuat dalam ujaran kebencian.
SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES
Authors: TRKOV Marija , AVGUŠTIN G. 
Published: December 2023

A method for specific detection of Salmonella spp. was developed based on 16S rRNA targeted PCR (polymerase chain reaction). A method based on recognition of specific region of 16S rRNA gene was published previously by Lin and Tsen in 1996. The sequence analysis of recently determined small subunit ribosomal genes from Salmonella spp. showed however, that the 16SF1 and 16SIII primers are not suitable for specific amplification of all Salmonella strains. 16SF1 was modified and a new forward PCR primer was designed. The specificity of the method was tested using 87 Salmonella strains and 30 non-Salmonella strains of the family Enterobacteriaceae and compared with two other previously published methods and found to be 100% specific. The reverse primer was also labelled with a tetramethylrhodamine isothyiocianate fluorescent dye and successfully used as a probe in in situ experiments.
MOLECULAR IDENTIFICATION AND CHARACTERIZATION OF AVIAN MYCOPLASMAS
Authors: DOVČ P. , BENČINA D. 
Published: December 2023

Serological techniques are recommended procedures for the identification of Mycoplasma species. They enable separation of mycoplasmas at the species level and are widely used for practical laboratory identification of the isolated microorganisms. However, complex growth media are required for the isolation of Mycoplasma species and some strains of pathogenic Mycoplasma species grow very fastidious and can not be cultured. Introduction of DNA based diagnostic methods in mycoplasma research enabled survey of the whole genome in a single assay and reveal also information about non-coding regions of the genome. Restriction enzyme analysis is a powerful method for differentiation of close related strains but requires high quality of DNA which can only be obtained from cultured mycoplasmas. The increasing number of cloned mycoplasma genomic fragments are successfully used for identification and differentiation of mycoplasma strains and species in hybridization studies. The polymerase chain reaction enables identification and characterization of mycoplasmas without culture directly from clinical material. Large genomic sequencing projects produced complete nucleotide sequence from two Mycoplasma species and several more are in progress. This data will open the possibility to understand better the biology of mycoplasmas.
Učinek gnojenja na hranilno vrednost krme s trajnega kraškega travnika. 1. botanična in kemična sestava, in vitro prebavljivost in vsebnost neto energije za laktacijo
Authors: VERBIČ Jože , VERBIČ Janko , BABNIK Drago 
Published: December 2023

Hranilno vrednost krme iz dolgoletnega gnojilnega poskusa smo ocenili na podlagi vsebnosti neto energije za laktacijo (NEL) in prebavljivosti organske snovi, izračunanih na osnovi kemične sestave in plina, ki se razvije med inkubacijo vzorcev z vampnim sokom in vitro. Primerjali smo krmo iz negnojene različice (0), različice, gnojene s fosforjem in kalijem (PK) in različice, gnojene s fosforjem, kalijem in dušikom (NPK). Skupni pridelek smo razdelili na trave, metuljnice in zeli in jih analizirali posebej. Gnojenje je le malo vplivalo na hranilno vrednost trav, metuljnic in zeli. Vsebnost neto energije za laktacijo je bila pri metuljnicah precej večja kot pri travah in zeleh: 5,95; 5,24 in 5,36 MJ na kg sušine. Zaradi razmeroma majhnih razlik v deležu metuljnic se večja vsebnost NEL v metuljnicah ni pokazala v razlikah med postopki. Gnojenje je izrazito povečalo pridelek NEL: 12000, 19500 in 34500 MJ na ha pri 0, PK in NPK postopku. Razlike med različicami so bile predvsem posledica različnih pridelkov sušine. Razlike v vsebnosti NEL so razmeroma malo prispevale k razlikam v pridelku NEL.
Učinek gnojenja na hranilno vrednost krme s trajnega kraškega travnika. 2. vsebnost makro- in mikroelementov
Authors: BABNIK Drago , ŽNIDARŠIČ-PONGRAC Vida , VERBIČ Janko , VERBIČ Jože 
Published: December 2023

Leta 1983 smo zasnovali večletni gnojilni poskus po načelu naključnih blokov v štirih ponovitvah. V letu 1994 smo zbrali vzorce krme na negnojeni različici (0), različici, gnojeni s fosforjem in kalijem (PK), ter različici, gnojeni s fosforjem, kalijem in dušikom (NPK). Vzorce prve, druge in tretje košnje smo ločili na trave, metuljnice in zeli ter ločeno določali vsebnosti Ca, P, Mg, K, Na, Zn, Mn, Fe, in Cu. Glede na delež posamezne botanične skupine smo izračunali tudi vsebnosti elementov v krmi. Gnojenje je značilno vplivalo na vsebnost večine elementov v travah, metuljnicah in zeleh vseh treh košenj. Gnojenje s PK je v krmi zmanjšalo vsebnost Mg in Mn ter povečalo vsebnost P in K. Pri gnojenju z NPK v primerjavi z negnojeno različico (0) se je v krmi zmanjšala vsebnost Fe, povečala pa vsebnost P, K in Zn. Gnojenje z dušikom (NPK vs. PK) je v krmi značilno povečalo vsebnost P, Mg, Zn in Mn ter zmanjšalo vsebnost Ca. Zaporedna košnja je značilno vplivala na vsebnost vseh analiziranih elementov v krmi razen na vsebnost Cu. Pri krmi druge in tretje košnje je bila vsebnost Ca, Mg in Mn večja kot pri krmi prve košnje. Največje vsebnosti P in Zn smo ugotovili v krmi tretje košnje. Metuljnice in zeli vsebujejo v primerjavi s travami dva- do trikrat več Ca, Mg, Zn in Cu. Zaradi tega se s spreminjanjem botanične sestave travne ruše precej spreminja tudi vsebnost teh elementov v krmi. Glede na potrebe krav v laktaciji smo ugotovili izrazito pomanjkanje Na, Zn in Cu, pomanjkanje P pa le v krmi z negnojenih parcel.
Genetska raznolikost bakterij v črevesu enakonožnih rakov (Isopoda, Crustacea)
Authors: KOSTANJŠEK Rok , ŠTRUS Jasna , AVGUŠTIN Gorazd 
Published: December 2023

V predelu zadnjega črevesa enakonožnih rakov vrste Porcellio scaber smo z vrstično elektronsko mikroskopijo odkrili paličaste bakterije, ki se s konci pritrjajo na kutikularne izrastke črevesne stene. Pritrjene bakterije so lahko del avtohtone črevesne mikroflore enakonožnih rakov, katere obstoj še ni bil potrjen. Ker opisanih bakterij ni mogoče gojiti s klasičnimi gojitvenimi tehnikami, smo iz spranih čreves petih zdravih odraslih rakov izolirali skupno DNK, iz katere smo s PCR namnožili bakterijske gene za 16S rRNK. Te gene smo vključili v plazmidni vektor in jih transformirali v kompetentne celice E.coli. Iz klonov izolirano plazmidno DNK smo razkrojili z restrikcijskima encimoma DdeI in TaqI in na podlagi restrikcijskih profilov z metodo UPGMA naredili fenogram. 37 preiskanih genov za 16S rRNK lahko na podlagi restrikcijskih profilov razdelimo v vsaj tri večje skupine, kar kaže, da se na kutikularne trne v črevesu P.scaber morda pritrjuje več različnih bakterijskih vrst.
Slovenian fermented milk with probiotics
Authors: VAHČIĆ Nada , HRUŠKAR Mirjana 
Published: December 2023

The concept of functional foods and probiotics is a novelty in former Eastern block. The Croatian and Slovenian dairy industries are the first that launched probiotic. In this work, samples of probiotic fermented milks purchased from Slovenian market were stored within 25 days at three different temperatures (4° C, 20° C, 37° C), and changes in acetaldehyde, ethanol and diacetyl were determined. Above chemical compounds have a high impact on the desired product flavour. At the same time sensory evaluation was carried out. Acetaldehyde and ethanol concentration were determined by aldehyde dehydrogenase and alcohol dehydrogenase methods. Diacetyl was measured by modification of the Hill’s colorimetric method. Sensory analysis was conducted by five member panel, using a scoring system with weighted factors in the 20-point scale. The concentration of these flavour compounds changed during storage depending on duration and temperature in depot. Acetaldehyde content decreased at each temperature level during 25-days storage. Diacetyl and ethanol content increased with duration at all temperature levels. Sensoric quality decreased with duration and was closely related to changes in the content of aroma compounds. Changes of all investigated parameters were pronounced on higher temperature levels. Thus, there were no significant changes during the refrigerated storage at 4° C while at the other two temperature levels results pointed out significant differences between the values before and after storage.
Vpliv bakterijskih cepiv na aerobno stabilnost silaže
Authors: KAPEL Damjan , STEKAR Jasna M.A. 
Published: December 2023

S statistično obdelavo objavljenih podatkov smo ugotavljali vpliv bakterijskih cepiv na aerobno stabilnost silaže. V silirani krmi s travinja z večjo vsebnostjo suhe snovi je bila aerobna stabilnost slabša ob dodatku cepiva, ki je vsebovalo homofermentativne mlečnokislinske bakterije, v primerjavi s silažo brez dodatka. Ob nizki vsebnosti sušine v silirani krmi s travinja je cepivo pri siliranju krme s travinja ugodno vplivalo tako na kakovost kot na aerobno stabilnost. Pri cepljenju koruze, sirka in žit se je kakovost silaž izboljšala, aerobna stabilnost pa je bila slabša. Izjema je bilo cepivo, ki je vsebovalo Lactobacillus plantarum in Enterococcus faecium, kjer se je aerobna stabilnost siliranih žit rahlo izboljšala. Heterofermentativna mlečnokislinska bakterija Lactobacillus buchneri je izboljšala tako kakovost kot tudi aerobno stabilnost koruzne silaže, siliranega sirka in siliranih žit ter se pokazala kot neučinkovita pri siliranju krme s travinja. Dodatek propionskokislinskih bakterij ni učinkoval na fermentacijo siliranih rastlin, ki se hitro aerobno kvarijo.
sulphur and chlorine contents of grass and maize silage
Authors: RAJČEVIČ Marija , VIDIC Alojz , KUBA Pavel , NEČEMER Marijan , KUMP Peter 
Published: December 2023

In seven defined samples of grass and in six samples of maize silage the contents of sulphur and chlorine were determined by two analytical procedures: both elements with X-ray fluorescence spectrometry (XRF), sulphur separately with inductively coupled plasma – atomic emission spectrometry (ICP-AES) and chlorine according to Volhard (Skoog and West, 1963; Official Journal of the EC, No.L. 155/13, 12/07/71). The average contents of sulphur in grass silage, g kg–1 DM, were 2.53 g and 1.91 g, in maize 0.87 g and 0.75 g by XRF and ICP-AES, respectively. The differences in the content of sulphur between the two methods were statistically significant (P < 0.01 and P < 0.05). The average contents of chlorine in grass silage, g kg–1 DM, were 7.69 g and 9.09 g, and in maize 2.00 g and 2.55 g by XRF and Volhard, respectively. The differences between the two methods were statistically significant (P < 0.01).
effect of diet containing arsenic (III) oxide on the traits of eggs
Authors: HOLCMAN Antonija , MALOVRH Špela , KNEZ Vanja 
Published: December 2023

The main objective of this study was to determine the effect of different arsenic concentration in feed on the physical traits of eggs. Twenty-four Rhode Island Red laying hens were divided into four equal groups. Three groups received feed mixture with additional arsenic in the form of arsenic (III) oxide in the following concentrations: 7.5 mg kg–1 (group 1), 15.0 mg kg–1 (group 2) and 30.0 mg kg–1 (group 3). The control group was fed on diet without additional arsenic. From the third to the seventh day eggs were collected and measured for physical traits. It has been established that during the trial period arsenic concentration in diet had a highly significant effect (P = 0.0001) on shell colour, egg mass, height of dense albumen, as well as on the number of Haugh units. The effect on shell mass and shell thickness was significant (P £ 0.01), while there was no significant influence on the colour of yolk. Changes in physical traits of eggs were not significant during this period. The only exception was the change in yolk colour, yet it was not due to arsenic, but to feeding mixture with added colouring the hens had been fed on prior to trial.
Priprava kvantitativne molekularno biološke metode za ugotavljanje števila celic vrste Salmonella enterica
Authors: TRKOV Marija , AVGUŠTIN Gorazd 
Published: December 2023

Pripravili smo molekularno biološko metodo za hitro štetje bakterijskih celic iz vrste Salmonella enterica, ki temelji na kompetitivni različici verižne reakcije s polimerazo (c-PCR). Uporabili smo začetna oligonukleotida MINF in MINR-Hex, ki sta zagotavljala specifičnost metode. Notranji standard DNK smo pripravili z encimskim razrezom in lepljenjem stranskih fragmentov pomnožka gena za 16S rRNK seva S. Agona (U92197). Notranji standard DNK je imel isti prepoznavni mesti za začetna oligonukleotida kot tarčna DNK, bil pa je za 27 bp krajši. Pomnožke c-PCR smo ločevali s kapilarno elektroforezo in rezultate obdelali z regresijsko analizo. Z opisano metodo in 5 × 10–5 redčenim notranjim standardom smo lahko učinkovito ugotavljali število celic vrste S. enterica v bujonski kulturi v območju med 1,2 × 104 in 6 × 105 celic na reakcijsko mešanico. Z metodo smo v preliminarnem poskusu ugotavljali število celic seva S. Livingstone v bujonski kulturi in rezultate primerjali z rezultati indirektne števne metode. Rezultati opisane metode c-PCR so bili 2,9 krat višji od rezultatov indirektnega štetja kolonij, vendar je glede na številne prednosti opisana metoda kljub temu uporabna za hitro, specifično in dovolj natančno ugotavljanje števila celic vrste S.enterica v naravnih mikrobioloških vzorcih.
Genetske razlike med istrsko pramenko, bovško ovco in jezersko-solčavsko ovco
Authors: KAVAR Tatjana , KOMPAN Drago , DOVČ Peter 
Published: December 2023

Z namenom, da bi ocenili genetsko sorodnost med tremi slovenskimi avtohtonimi pasmami ovc smo proučevali razlike na mikrosatelitnih lokusih: MAF214, OarCP49, OarFCB11, MAF65, McM42, TGLA53, McM527 in OarAE119. Genotipizacija 95 istrskih pramenk (ISP), 272 bovških ovc (B) in 42 jezersko-solčavskih ovc (JS) je pokazala, da so za vse tri pasme večinoma značilni enaki aleli, oz. še več, 12 alelov je pogostih (> 10 %) v vseh treh pasmah. Na vsakem od lokusov smo našli tudi 1–5 privatnih alelov. Osem alelov je bilo specifičnih za ISP, 8 za B in 3 za JS. Korespondenčna analiza in analiza uvrščanja genotipov osebkov v ustrezno pasmo kažeta, da se večina osebkov pasme ISP uvršča v svojo skupino, medtem ko se porazdelitvi B in JS, zaradi več zelo podobnih genotipov v obeh pasmah, delno prekrivata. Ocene genetskih razdalj potrjujejo višjo sorodnost med B in JS (FST = 0,044) kot med ISP in B (FST = 0,064) ali med ISP in JS (FST = 0,075). Okrog 6 % delež variance, ki izhaja iz razlik med pasmami (FST vrednost), kaže, da razlike med pasmami niso prav velike. Menimo, da so rezultati v skladu s hipotezo o skupnem nastanku B in JS iz domače primitivne bele ovce, oz. nastanku ISP iz nekoliko bolj oddaljene pramenke »Zackel« ovce. Šele nadaljnje analize pa bodo lahko osvetlile vplive padovanske in bergamaške ovce, ki sta verjetno sodelovali pri nastanku JS, pa tudi vplive drugih sojev pramenk na ISP.
Vpliv peroralno dodanih kostanjevih taninov na dnevni prirast in sestavo iztrebkov pri ovcah in kozah
Authors: KOMPREJ Andreja , OREŠNIK Andrej , POGAČNIK Milan , VIDRIH Anton 
Published: December 2023

V raziskavi smo proučevali učinek ovcam in kozam v obrok dodanega izvlečka taninov lesa pravega kostanja (Castanea sativa Mill.), ki vsebuje 74 % pretežno hidrolizirajočih taninov. V poskus smo vključili 21 jagnjic in 10 koz, ki so se pasle na kraškem pašniku. Trinajstim jagnjicam in petim kozam smo vsake tri dni peroralno dajali pripravek kostanjevih taninov v odmerku 0,5 g kg–1 telesne mase. Osem jagnjic in pet koz smo spremljali kot kontrolni skupini. Živali so se pasle skupaj v ogradah na istem pašniku. Poskus je trajal 68 dni. Jagnjice poskusne skupine so na paši priraščale v povprečju 37 g dan–1, jagnjice kontrolne skupine pa 18 g dan–1. Razlike v dnevnih prirastih jagnjic niso bile statistično značilne. V prvem obdobju (35 dni) je bilo v iztrebkih jagnjic poskusne skupine manj SS (189–217 g kg–1 vs. 195–229 g kg–1), več SB (177–157 g kg–1 SS vs. 170–142 g kg–1 SS) in več taninov (0,77–0,61 % v SS vs. 0,73–0,52 % v SS) kot v iztrebkih jagnjic kontrolne skupine. Povezava med vsebnostjo taninov in surovih beljakovin v iztrebkih jagnjic je bila statistično značilna (r = 0,92, P < 0,05). Rezultati kažejo, da dodani kostanjevi tanini vplivajo na sestavo blata, kar je verjetno posledica mesta prebave zaužitih hranil. Obe skupini koz sta v poskusu hujšali (–79 g dan–1). V iztrebkih koz je bilo več suhe snovi (192–297 g kg–1) in manj surovih beljakovin (108–144 g kg-1 SS) ter taninov (0,24–0,48 % v SS) kot v iztrebkih jagnjic.
Log-konkavni karakter mikrobne rastne krivulje brez lag-faze
Authors: CEDILNIK Anton , GORUP Eva C. 
Published: December 2023

Minotov zakon, po katerem je relativna stopnja rasti padajoča na vsem območju, kjer je mikrobna rastna krivulja naraščajoča, razširimo še na območje, kjer opazovana kultura umira. Pokažemo, da je rastna krivulja, za katero velja ta zakon, logaritmično konkavna in da ima zato vselej obliko , kjer je padajoča funkcija. Konec lag-faze definiramo kot začetek logaritmične konkavnosti rastne krivulje. V nadaljevanju naštejemo ostale splošne lastnosti takih rastnih krivulj, utemeljimo osnovni princip aproksimacije konkretnih podatkov in predlagamo preprost model.
incidence of the root-knot nematode Meloidogyne incognita and Meloidogyne hapla in Slovenia
Authors: ŠIRCA Saša , UREK Gregor , KARSSEN Gerrit 
Published: December 2023

The root knot nematode Meloidogyne incognita (Kofoid and White) Chitwood, 1949 is one of the most harmful and most ubiquitous species in the genus Meloidogyne. It was detected very often in the greenhouses of northern Europe, but in the open field it is restricted to the southern parts of Europe. While this species has been recorded in almost all parts of the world and also in some parts of the former Yugoslavia, there is no data about its presence in Slovenia. M. incognita is able to parasitize about 700 plant hosts and varieties including the majority of economically important crops. In the late summer of 2002, nematodes of M. incognita were isolated from the roots of hot pepper plants, Capsicum annum L., from the greenhouse situated in Portorož at the Adriatic Coast, Slovenia. The nematode was morphologically identified as M. incognita and confirmed by isozyme gel electrophoresis (PhastSystem, Pharmacia). To our knowledge, this is the first report of M. incognita in Slovenia. Beside this finding, M. hapla was also found for the first time in the open field in Slovenia; until now it has been detected on different host plants grown only in greenhouses. In October 2002, M. hapla was isolated from galls of the sweet pepper grown in the open field in Ljubljana situated in the central part of Slovenia.