bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospects for using flow cytometry in the quality control of live plague vaccines
Authors: Abzaeva N. V. , Kuznetsova I. V. , Gostischeva S. E. , Zhirov A. M. , Kovalev D. A. , Kostrominov A. V. , Fisun A. A. , Ivanova G. F. 
Published: December 2023

Scientific relevance. The number of live bacteria is a quality parameter controlled at all stages of live plague vaccine production. Currently, live microbial cell counting uses a bacteriological method. However, flow cytometry has the potential to increase analytical accuracy and reduce testing time.Aim. This study aimed at testing the applicability of flow cytometry to assessing the quality of live plague vaccines.Materials and methods. The study quantified live microbial cells in 5 experimental batches of live plague vaccine as part of their quality control using the bacteriological method according to the State Pharmacopoeia of the Russian Federation (FS.3.3.1.0022.15). Cytofluorometry of the samples used the SynaptoGreen fluorescent dye.Results. The study quantified live microbial cells in live plague vaccine samples using the bacteriological method and flow cytometry. The results obtained by the bacteriological method ranged from 27.8±2.2 to 56.5±3.1% with an average of 39.8±5.4%. The results obtained by flow cytometry ranged from 29.2±1.2 to 59.1±2.1% with an average of 41.7±5.5%. The statistical analysis showed no significant difference between the results of vaccine quality control by both methods, as well as a high coefficient of determination.Conclusions. The results show that flow cytometry is an appropriate method for the quantification of live microbial cells as part of the quality control of plague vaccines. Being quick, easy, and highly informative, flow cytometry is preferable to traditional methods.
Experimental evaluation of the possibility of determining bacterial endotoxins in typhoid Vi polysaccharide vaccines using the gel-clot test
Authors: Abramtseva M. V. , Alekhina N. S. , Kolyshkina E. D. 
Published: December 2023

Scientific relevance. Currently, only the rabbit pyrogen test is used to test the Vianvac® typhoid Vi polysaccharide vaccine for pyrogenicity. As part of the product specification file, the gel-clot test for bacterial endotoxins (BE) will improve the reliability of quality control, as well as harmonise the requirements for the vaccine with the requirements outlined for this group of medicinal products by leading world pharmacopoeias.Aim. This study aimed at an experimental assessment of the applicability of the gel-clot test to the quantification of BE in the typhoid Vi polysaccharide vaccine.Materials and methods. This study used samples from 5 batches of the typhoid Vi polysaccharide vaccine (0.5 mL/dose, solution for subcutaneous injection), LAL and TAL reagents. The analysis included the gel-clot test and the pyrogenicity test according to the State Pharmacopoeia of the Russian Federation (OFS.1.2.4.0006.15 and OFS.1.2.4.0005.15, respectively).Results. According to calculations, the BE limit for the tested vaccine was 96 EU/mL, and the maximum valid dilution (MVD) was 3200. The authors determined the regulatory requirements for typhoid Vi polysaccharide vaccine quality in terms of BE (not more than 48 EU/dose). The in vitro BE tests were positive at vaccine dilutions of 1/16 to 1/32 and negative at 1/64 to 1/256. The authors selected and validated a working vaccine dilution of 1/128. The BE content measured in the tested samples ranged from 0.24 to 0.48 EU/dose. The in vivo pyrogen tests were positive at dilutions of 1/16 to 1/128 and negative at 1/256 in all experiments with samples from 5 vaccine batches at dilutions ranging from 1/16 to 1/256.Conclusions. This study has experimentally proven that the gel-clot test can quantify BE in the typhoid Vi polysaccharide vaccine. The authors have recommended introducing the gel-clot BE test in the monograph of the State Pharmacopoeia of the Russian Federation on the typhoid Vi polysaccharide vaccine (FS.3.3.1.0012.15).
Validation studies of the spiral plating method to determine the potency of lactobacillus-containing probiotics
Authors: Voropaev A. A. , Fadeikina O. V. , Evlashkina V. F. , Bohanova T. D. , Davydov D. S. 
Published: December 2023

Scientific relevance. Viable cell counting is an important microbiological test for the quality assessment of medicinal products containing live microorganisms. To optimise labour and material costs and enhance testing precision and reproducibility, it is practical to use partially automated instrumental methods, such as the spiral plating method.Aim. The aim was to conduct a validation study of the spiral plating method for assessing the potency of biologicals containing live bacterial cells, using a lactobacillus-containing probiotic medicinal product as a case study.Materials and methods. This study used a culture of Lactiplantibacillus plantarum isolated from a probiotic medicinal product. Spiral plating on agar-based biological culture media was performed using an automatic Eddy Jet 2 plating system. The study used an IUL Flash & Go colony counter for automatic reporting of the results. The validation study was conducted according to the general chapter on the validation of microbiological testing methods (OFS.1.1.0021.18) of the State Pharmacopoeia of the Russian Federation.Results. According to the validation results for the main parameters, the spiral plating method had the range of 104–105 CFU/mL; the limit of quantification of 102 CFU/mL; and the coefficient of linear determination, R2, of 0.99. The accuracy of the spiral plating method in determining the potency of the test sample was 93%; the repeatability was 4.9%.Conclusions. The study results confirm the similarity of the spiral plating method with an automatic colony counter to Koch’s plating method, which is currently used in accordance with the State Pharmacopoeia of the Russian Federation, in terms of the validated parameters. Therefore, spiral plating can be used to evaluate the potency of lactobacillus-containing probiotics. Spiral plating can help improve the cost-effectiveness and accuracy of testing.
Morphofunctional characteristics of <i>Trichinella spiralis</i> capsules and larvae obtained from the lynx in spontaneous infection and experimental infection of white rats
Authors: Zhdanova O. B. , Okulova I. I. , Uspensky A. V. , Chasovskikh O. V. , Kozlova A. V. 
Published: December 2023

The purpose of the research is a comparative study of morphological changes in Trichinella spiralis capsules and larvae in the lynx and laboratory animals.Materials and methods. The experiment used striated muscle tissue from spontaneously infected lynxes and from white rats experimentally infected with T. spiralis larvae isolated from wild animals in the Kirov Region. Morphometric studies of T. spiralis capsules and larvae in the lynx muscle tissue were conducted on temporary histologic specimens.Results and discussion. Lemon-shaped (50%) and oval (40%) capsules were found in the entire muscle mass of the white rats, and only 10% of the larvae were round in shape. The lynx was not found to have lemon-shaped capsules; oval (60%) and round (40%) capsules predominated. The studies show that the parasite capsule size and shape indicate the adaptive abilities of T. spiralis to various types of host muscle tissue and their morphological characteristics, and are largely determined by the symplast structure and size.
A method for protecting herd horses from blood-sucking insects that ensures organic food production
Authors: Reshetnikov A. D. , Barashkova A. I. 
Published: December 2023

The purpose of the research is to develop a method for protecting herd horses from dangerous blood-sucking dipterans that ensures organic meat production without pesticides.Materials and methods. Dangerous dipterans were collected and counted using an entomological net. The Diptera species identification was determined using the morphological keys. A new technology for protecting herd horses from harmful insects without pesticides used was implemented at Horobut LLC in the Megino-Kangalassky District, the Republic of Sakha (Yakutia) in 2021–2023. Pursuant to the Assignment and Regulations, a patent search was performed in the Russian scientific and technical literature on the subject “Develop methods for creating technologies to control and prevent animal diseases that ensure organic food production from conventional agricultural sectors in the Far North”.Results and discussion. The number of mosquitoes and horseflies that attack herd horses in Central Yakutia was established. In the morning and evening peaks, the number of mosquitoes for a 5-minute count was 397–456 mosquitoes belonging to 15 species of three genera: Aedes (Ochlerotatus), Culiseta and Anopheles. During a 15-minute count, the bait horse was attacked by 83 to 107 specimens of horseflies of 21 species and one subspecies of two genera, Hybomitra and Chrysops. For the first time in Central Yakutia, a method of protecting herd horses from attacks by blood-sucking insects was used with positive results without spraying animals with insecticides.
DEVELOPMENT AND EXPERIMENTAL REPLICATION OF THE MODEL OF PARTIAL TRACHEAL STENOSIS IN YOUNG SEXUALLY IMMATURE WHITE RATS
Authors: Molochek Yu.A. , Levchuk N.I. , Makarenko O.M. 
Published: December 2023

This work is devoted to the development of two models of experimental replication of stenosis in the upper part of the trachea with different degrees of compression of this organ in sexually immature (one-month-old) white Wistar rats. Considering the different degrees of laryngomalacia in newborns, we developed two models: with a lower (20%) and higher (30%) degree of airway obstruction. In the first version of modeling, this was achieved by applying the ligature at the level between 1 and 2, or 2 and 3 cartilaginous semi-rings of the trachea, in the second – by applying it directly to the 2 cartilaginous semi-ring of the trachea. Both models were performed to study the state of the organism in conditions of hypoxic hypoxia in laboratory animals.
The developed modeling methods differ from each other not only in the place of application of the compressing ligature, but also in the mechanism of compression development. In the case of applying a ligature to the intercartilaginous ligaments (20% stenosis), soft tissues are compressed and both semi-rings of the trachea are brought together. In the second variant of modeling, the pathophysiological mechanism of stenosis consists not only in the compression of the cartilaginous semi-ring of the trachea, but also in the process of invagination of the dorsal, i.e., elastic part of the trachea into the lumen of this hollow organ. As a result, a more intense variant of tracheal stenosis in experimental animals is achieved. Another important component of the developed models is the possibility at different stages of the experiment to eliminate the compressive effect of the ligature on the trachea and to study the state of various organs and tissues of animals in the early or delayed post-hypoxic periods. This leads to a significant increase in the value or efficiency of conducting this kind of experiments, taking into account the fact that hypoxic and posthypoxic periods can be studied on the same experimental animal. Both exotracheal modeling methods are fundamentally different from the endotracheal variant of experimentally reproducing stenosis. With the latter, pathological structural and functional disorders of the cells of the mucous membrane, the connective apparatus of the trachea and smooth muscle elements, the development of inflammation, and the progression of irreversible changes in the cellular and organ structures of the trachea are observed. In addition, simulation of the specified variant of stenosis requires the use of expensive equipment – a laryngoscope and some other modern technologies. Both variants of exotracheal modeling of tracheal stenosis, proposed by us, are devoid of all these shortcomings. At the same time, they enable to achieve the same degree of stenosis of this organ in animals as with the endotracheal variant.
PRAKSIS PEDAGOGI MULTIKULTURAL SEKOLAH DASAR DI KABUPATEN SINTANG
Authors: Dike Daniel , Parida Lusila 
Published: December 2023

ABSTRAKPenelitian ini bertujuan memaparkan pemahaman dan pengalaman siswa dan guru di sekolah dasar Katolik dan Sekolah Dasar Negeri terkait praksis pedagogi multikultural di sekolah dasar. Desain penelitian menggunakan penelitian case study untuk memetakan dan memaknai pengalaman personal dan komunal warga sekolah di SD Panca Setya 1 Sintang dan SDN Negeri 5 Sintang. Hasil penelitian menunjukan bahwa pada level pemahaman multikultural guru dan siswa memahami perbedaan sebagai dasar membangun persaudaraan dan kekeluargaan sebagai karakter sosial dan budaya bangsa melalui Pancasila. Pada level kesadaran nilai dan sikap multikultural warga sekolah menghidupi perbedaan sebagai cara hidup yang telah membudaya dan memperkuat identitas kelompok yang beragam dan pemenuhan hak-hak dasar warga sekolah. Pedagogi multikultural yang cocok dikembangkan di sekolah dasar dilaksanakan melalui integrasi budaya dengan semangat pluralisme egalitarian berdasarkan nilai-nilai harmoni, musawarah, gotong-royong, dan sikap toleransi yang mengakar kuat dalam kesadaran tradisi dan sejarah.Kata Kunci: Pedagogi, multikultural, sekolah dasarABSTRACTThis research aims to describe the understanding and experiences of students and teachers in Catholic elementary schools and state elementary schools regarding the praxis of multicultural pedagogy in elementary schools. The research design uses case study research to map and interpret the personal and communal experiences of school residents at SD Panca Setya 1 Sintang and SDN Negeri 5 Sintang. The research results show that at the level of multicultural understanding, teachers and students understand differences as the basis for building brotherhood and kinship as the social and cultural character of the nation through Pancasila. At the level of awareness of multicultural values and attitudes, the school community embraces differences as a way of life that has entrenched and strengthened the identity of diverse groups and fulfilled the basic rights of the school community. Multicultural pedagogy that is suitable to be developed in elementary schools is implemented through cultural integration with a spirit of egalitarian pluralism based on the values of harmony, deliberation, mutual cooperation and an attitude of tolerance that is deeply rooted in an awareness of tradition and history.Keywords: Pedagogy, multicultural, elementary school
PEMANFAATAN MEDIA AUDIO VISUAL BAGI GURU PAK DAN KATEKIS SEBAGAI WUJUD TRANSFORMASI MENUJU ERA SOCIETY 5.0
Authors: Arifman Halawa Arius , Zulkarnain Rezkie , Kurniati Yusi 
Published: December 2023

ABSTRAKStudi ini memfokuskan tentang upaya optimalisasi pemanfaatan media audio visual yang digunakan para guru Pendidikan Agama Katolik dan katekis menuju Era Society 5.0. Penelitian ini menggunakan Mixed Method tipe Covergent Parallel Design, dimana peneliti menggunakan data hasil angket dan wawancara secara bersamaan untuk mengidentifikasi seberapa kuat pemanfaatan media audio visual tersebut digunakan dan bagaimana cara memanfaatkan media tersebut dalam berkatekese. Hasil membuktikan bahwa para guru dan katekis di Keuskupan Agung Pontianak mampu memanfaatkan media audio visual secara maksimal dan tergolong kuat. Hal ini menjelaskan bahwa mereka dapat membuat media yang mampu menarik minat umat terhadap materi katekese seperti pengetahuan tentang iman Katolik, doa harian maupun kata-kata bijak. Adapun media audio visual yang digunakan berupa media interaktif yang menarik seperti Power Point/Canva untuk media presentasi dan aplikasi TikTok/Kinemaster untuk membuat video/film. Selain itu untuk kemudahan akses, pengajaran dilakukan dengan menyebarkan media tersebut secara virtual conference dengan aplikasi meeting online. Kata Kunci: Media Audio Visual; Guru PAK & Katekis; Era Society 5.0ABSTRACTThis study focuses on efforts to optimize the use of audio-visual media used by Catholic teachers and catechists towards the Era of Society 5.0. This research uses Mixed Method type Covergent Parallel Design, where researchers use questionnaire and interview data simultaneously to identify how strong the utilization of audio visual media is used and how to utilize the media in blessing. The results prove that teachers and catechists in the Archdiocese of Pontianak are able to utilize audio-visual media maximally and are classified as strong. This explains that they can create media that can attract people's interest in catechesis materials such as knowledge about the Catholic faith, daily prayers and words of wisdom. The audio visual media used is in the form of attractive interactive media such as Power Point/Canva for presentation media and TikTok/Kinemaster applications for making videos/films. In addition, for ease of access, teaching is carried out by distributing the media in virtual conferences with online meeting applications.Keywords: Audio Visual Media; Catholic teachers and catechists; Era Society 5.0
A Factors Influencing the Incidence of Premature Rupture of Membrane (Prom) in Maternity Mothers
Authors: Hipni Rubiati , Isnawati Isnawati 
Published: December 2023

Abstract

Premature rupture of membranes (KPD) or Premature Rupture Of The Membrane (PROM) is a rupture of the membranes before delivery if the opening in primi is less than 3 cm and in multi it is less than 5 cm. Based on the latest data obtained at Idaman Banjarbaru Hospital, mothers who experienced KPD in 2016 were 145 mothers with KPD out of 1594 mothers giving birth (9%) and in 2017 there was an increase of 242 mothers experiencing KPD out of 1974 mothers giving birth (12.25%). This study aimed to discover the factors that influence the incidence of premature rupture of membranes (PROM) in women giving birth. Research method: The research design used in this research is descriptive. The population in this study were all mothers with premature rupture of membranes at Idaman Banjarbaru Hospital in 2017 as many as 242 people. The sampling technique used systemic random sampling with a total sample of 152 people—a collection tool for medical record data documentation. The analysis used is univariate analysis with a percentage formula. The results showed that the number of mothers giving birth with KPD based on the causal factors at Idaman Banjarbaru Hospital in 2017 was caused by abnormalities in the location of the fetus as many as 8 people (5.3%), age factor <20 &> 35 years as many as 34 people (22.4%) ), parity factors 1&>4 were 80 people (52.6%), and multiple pregnancy factors were 6 people (3.9%). Suggestions for routine ANC in pregnant women and the need for further research on other factors that can cause premature rupture of membranes.

Keywords: Factors, KPD, mothers giving birth
PERKEMBANGAN INSTITUSI-INSTITUSI PENDIDIKAN PADA MASA KLASIK
Authors: Holilah Noer 
Published: December 2023

Telaah sejarah institusi-institusi pendidikan dalam tradisi islam penting untuk dijadikan model pengembangan pendidikan di era modern. Hal tersebut dikarenakan isntitusi Pendidikan yang menjadi bagian dari transmisi keilmuan merupakan sebuah sarana dalam membangun pendidikan islam untuk mengembangkan intelektual umat muslim. Sejarah institusi pendidikan dalam transmisi keilmuan itu sendiri mulai menunjukkan eksistensinya dari masa klasik yang dimulai sejak diutusnya Nabi Muhammad shallallahu ‘alaihi wasallam menjadi rasul hingga runtuhnya baghdad. Hal tersebut juga menjadi cikal bakal perkembangan institusi pendidikan hingga saat ini. Corak institusi pendidikan yang berkembang pada saat itu masih berupa tempat-tempat yang sederhana karena keterbatasan yang ada, seperti rumah-rumah para sahabat, kuttab, masjid, masjid khan dan madrasah. Proses pembelajaran di dalamnya juga masih menggunakan sistem dekte, menghafal dan tulis menulis. Dengan keterbatasan yang ada, hal itu tidak menjadikan proses transmisi keilmuan dalam institusi Pendidikan menjadi lemah dan runtuh, akan tetapi menjadi awal kesuksesan dunia pendidikan islam dan dijadikan sebagai role mode hingga saat ini. Tulisan kali ini akan membahas tentang sejarah pendidikan islam khususnya pada sejarah perkembangan institusi pendidikan pada masa klasik. Tujuan dari penulisan ini yaitu untuk melacak kembali dinamika transmisi keilmuan yang di dalamnya terdapat institusi pendidikan yang menjadi wadah atau material dalam proses transmisi keilmuan.
Kata kunci : perkembangan, institusi pendidikan, masa klasik
PENGGUNAAN METODE DEMONSTRASI DALAM MENINGKATKAN HASIL BELAJAR SISWA PADA MATA PELAJARAN MATEMATIKA KELAS V MI NEGERI 4 KERINCI
Authors: Amrul Amrul 
Published: December 2023

Penelitian ini dilakukan untuk mengetahui apakah penggunaan metode demonstrasi dapat meningkatkan hasil belajar siswa pada mata pelajaran matematika kelas V MIN 4 Kerinci. Jenis penelitian yang digunakan adalah penelitian Tindakan kelas (PTK) dan dilaksanakan dalam dua siklus, setiap siklus dilaksanakan dua kali tatap muka. Subjek penelitian yakni siswa kelas V MIN 4 Desa Tanjung Genting Kerinci. Sedangkan metode pengumpulan data yang digunakan dalam penelitian ini adalah observasi, tes, dan dokumentasi. Hasil penelitian menunjukkan bahwa adanya peningkatan hasil belajar siswa pada proses pembelajaran dengan menggunakan metode demonstrasi. Hal ini dapat dilihat dari ketuntasan KKM siswa pada setiap siklusnya. Rata-rata pada siklus I sebesar 58%, dan pada siklus II sebesar 74%. Dengan demikian, dapat disimpulkan bahwa proses pembelajaran menggunakan metode demonstrasi dapat meningkatkan hasil belajar siswa MIN 4 Desa Tanjung Genting Kerinci.
Kata kunci: demonstasi, metode pembelajaran, hasil belajar
PENGEMBANGAN SUMBER DAYA MANUSIA BERBASIS AL-QUR’AN DAN AL-HADIS
Authors: Nurmadiah Nurmadiah , Maimunah Maimunah , Indrawan Irjus 
Published: December 2023

Sumber daya manusia merupakan kunci pokok yang harus diperhatikan dengan segala kebutuhannya. SDM sebagai sumber daya terpenting organisasi yang mempunyai implikasi bahwa pengembangannya harus dianggap sebagai investasi. Sehingga penanganannya harus dilakukan seefektif mungkin. Relevansi mempelajari pengembangan SDM dapat dilihat dari sebagian besar struktur organisasi saat ini. Salah satu unsur yang cukup menentukan dalam upaya manajemen sumber daya manusia pada suatu organisasi adalah manusianya. Dengan seperangkat organ tubuh yang diberikan oleh Allah swt kepada manusia, manusia mempunyai daya atau potensi yang apabila dikembangkan akan menjadi sumber daya manusia yang berkualitas, dan akan menjadikan manusia yang sadar akan tanggung jawabnya, baik tanggung jawabnya sebagai hamba Allah swt dan sebagai khalifah Allah swt.
PENERAPAN MANAJEMEN WAKTU PADA LEMBAGA PENDIDIKAN SMP N 4 dan MTs N 1 Pekanbaru
Authors: Wahyuni Suci 
Published: December 2023

Waktu adalah sumber daya yang unik. Setiap orang memilikinya dalam jumlah yang sama. Waktu tidak dapat dibeli dan setiap hal memakan waktu. Pemecahan nyata satu-satunya adalah penggunaan waktu dengan lebih baik. Setiap orang harus belajar untuk mengelolah penggunaan waktu mereka. Praktik mengelolah waktu adalah kebiasaan yang dapat dipelajari. Tujuan penelitian ini adalah untuk memperdalam pengetahuan mengenai manajemen waktu. Terutama penerapan manajemen waktu pada lembaga pendidikan (SMP N 4 dan MTs N 1 Pekanbaru). Jenis penelitian ini adalah penelitian kualitatif, subjek penelitian ini adalah kepala sekolah, tenaga pendidik, tenaga kependidikan, peserta didik, sedangkan objek penelitian ini adalah penerapan manajemen waktu pada lembaga pendidikan. Teknik yang digunakan dalam mengumpulkan data adalah wawancara, observasi, dan dokumentasi. Adapaun hasil penelitian ini adalah penerapan manajemen waktu pada lembaga pendidikan (SMP N 4 dan MTs N 1 Pekanbaru) “sudah optimal”. Hal ini terlihat dari hasil wawancara peneliti dengan subjek penelitian dan berdasarkan hasil observasi langsung peneliti.