bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graft-versus-host-disease prophylaxis with ATG or PTCY in patients with lymphoproliferative disorders undergoing reduced intensity conditioning regimen HCT from one antigen mismatched unrelated donor
Authors: Paviglianiti Annalisa , Ngoya Maud , Peña Marta , Boumendil Ariane , Gülbas Zafer , Ciceri Fabio , Bonifazi Francesca , Russo Domenico , Fegueux Nathalie , Stolzel Friedrich , Bulabois Claude Eric , Socié Gerard , Forcade Edouard , Solano Carlo , Finel Hervé , Robinson Stephen , Glass Bertram , Montoto Silvia 
Published: February 2024

AbstractPost-transplant cyclophosphamide (PTCY) has been introduced as graft-versus-host disease (GvHD) prophylaxis in mismatched and matched unrelated hematopoietic cell transplant (HCT). However, data comparing outcomes of PTCY or ATG in patients undergoing a 1 antigen mismatched HCT for lymphoproliferative disease are limited. We compared PTCY versus ATG in adult patients with lymphoproliferative disease undergoing a first 9/10 MMUD HCT with a reduced intensity conditioning regimen from 2010 to 2021. Patients receiving PTCY were matched to patients receiving ATG according to: age, disease status at transplant, female to male matching, stem cell source and CMV serology. Grade II-IV acute GvHD at 100 day was 26% and 41% for the ATG and PTCY group, respectively (p = 0.08). Grade III–IV acute GvHD was not significantly different between the two groups. No differences were observed in relapse incidence, non-relapse mortality, progression-free survival, overall survival and GvHD-relapse-free survival at 1 year. The cumulative incidence of 1-year extensive chronic GvHD was 18% in the ATG and 5% in the PTCY group, respectively (p = 0.06). In patients with lymphoproliferative diseases undergoing 9/10 MMUD HCT, PTCY might be a safe option providing similar results to ATG prophylaxis. Due to the limited number of patients, prospective randomized trials are needed.
Hematopoietic stem cell transplantation for pediatric patients with non-anaplastic peripheral T-cell lymphoma. An EBMT pediatric diseases working party study
Authors: Moser Olga , Ngoya Maud , Galimard Jacques-Emmanuel , Dalissier Arnaud , Dalle Jean Hugues , Kalwak Krzysztof , Wössmann Wilhelm , Burkhardt Birgit , Bierings Marc , Gonzalez-Vicent Marta , López Corral Lucía , Mellgren Karin , Attarbaschi Andishe , Bourhis Jean Henri , Carlson Kristina , Corbacioglu Selim , Drabko Katarzyna , Sundin Mikael , Toporski Jacek , Cario Gunnar , Kontny Udo 
Published: February 2024

AbstractPeripheral T-cell lymphomas (PTCL) other than anaplastic large-cell lymphoma are rare in children, and the role of hematopoietic stem cell transplantation (HSCT) has not been clarified yet. In a retrospective analysis of registry-data of the European Society for Blood and Marrow Transplantation we analyzed 55 patients aged < 18 years who received allogeneic (N = 46) or autologous (N = 9) HSCT for PTCL. Median age at HSCT was 13.9 years; 33 patients (60%) were in first remission, and 6 (19%) in progression at HSCT. Conditioning was myeloablative in 87% of the allogeneic HSCTs and in 27 (58.7%) based on total body irradiation. After allogeneic HSCT the 5-year overall- and progression-free survival was 58.9% (95% CI 42.7–71.9) and 52.6% (95% CI 36.8–66.1), respectively. 5-year relapse incidence was 27.6% (95% CI 15.1–41.6), the non-relapse mortality rate was 19.8% (95% CI 9.7–32.6). Five of the six patients with progression at HSCT died. Seven of nine patients after autologous HSCT were alive and disease-free at last follow-up. Our data suggest a role of allogeneic HSCT in consolidation-treatment of patients with high-risk disease, who reach at least partial remission after primary- or relapse-therapy, whereas patients with therapy-refractory or progressive disease prior to transplantation do not profit from HSCT.
Convenient synthesis and delivery of a megabase-scale designer accessory chromosome empower biosynthetic capacity
Authors: Ma Yuan , Su Shuxin , Fu Zongheng , Zhou Chu , Qiao Bin , Wu Yi , Yuan Ying-Jin 
Published: February 2024

AbstractSynthetic biology confers new functions to hosts by introducing exogenous genetic elements, yet rebuilding complex traits that are based on large-scale genetic information remains challenging. Here, we developed a CRISPR/Cas9-mediated haploidization method that bypasses the natural process of meiosis. Based on the programmed haploidization in yeast, we further developed an easy-to-use method designated HAnDy (Haploidization-based DNA Assembly and Delivery in yeast) that enables efficient assembly and delivery of large DNA, with no need for any fussy in vitro manipulations. Using HAnDy, a de novo designed 1.024 Mb synthetic accessory chromosome (synAC) encoding 542 exogenous genes was parallelly assembled and then directly transferred to six phylogenetically diverse yeasts. The synAC significantly promotes hosts’ adaptations and increases the scope of the metabolic network, which allows the emergence of valuable compounds. Our approach should facilitate the assembly and delivery of large-scale DNA for expanding and deciphering complex biological functions.
Novel variant of FBN2 in a patient with congenital contractual arachnodactyly
Authors: Nakama Mina , Miwa Yuki , Manabe Sayaka , Shimamoto Shigeru , Ohnishi Hidenori 
Published: February 2024

Abstract
Congenital contractual arachnodactyly (CCA) is a genetic connective tissue disorder that is characterized by arachnodactyly, kyphoscoliosis, marfanoid habitus, and crumpled ears. We report a case of a boy with suspected Marfan syndrome. Genetic analysis revealed c.3207_3217+9del in a heterozygote form of the fibrillin-2 (FBN2) gene. This patient was diagnosed with CCA based on his phenotype, and the pathogenicity of this variant was classified according to cDNA analysis and protein modeling.
Re-evaluation of battery-grade lithium purity toward sustainable batteries
Authors: Choe Gogwon , Kim Hyungsub , Kwon Jaesub , Jung Woochul , Park Kyu-Young , Kim Yong-Tae 
Published: February 2024

AbstractRecently, the cost of lithium-ion batteries has risen as the price of lithium raw materials has soared and fluctuated. Notably, the highest cost of lithium production comes from the impurity elimination process to satisfy the battery-grade purity of over 99.5%. Consequently, re-evaluating the impact of purity becomes imperative for affordable lithium-ion batteries. In this study, we unveil that a 1% Mg impurity in the lithium precursor proves beneficial for both the lithium production process and the electrochemical performance of resulting cathodes. This is attributed to the increased nucleation seeds and unexpected site-selective doping effects. Moreover, when extended to an industrial scale, low-grade lithium is found to reduce production costs and CO2 emissions by up to 19.4% and 9.0%, respectively. This work offers valuable insights into the genuine sustainability of lithium-ion batteries.
Melt-quenched carboxylate metal–organic framework glasses
Authors: Kim Minhyuk , Lee Hwa-Sub , Seo Dong-Hyun , Cho Sung June , Jeon Eun-chae , Moon Hoi Ri 
Published: February 2024

AbstractAlthough carboxylate-based frameworks are commonly used architectures in metal-organic frameworks (MOFs), liquid/glass MOFs have thus far mainly been obtained from azole- or weakly coordinating ligand-based frameworks. This is because strong coordination bonds of carboxylate ligands to metals block the thermal vitrification pathways of carboxylate-based MOFs. In this study, we present the example of carboxylate-based melt-quenched MOF glasses comprising Mg2+ or Mn2+ with an aliphatic carboxylate ligand, adipate. These MOFs have a low melting temperature (Tm) of 284 °C and 238 °C, respectively, compared to zeolitic-imidazolate framework (ZIF) glasses, and superior mechanical properties in terms of hardness and elastic modulus. The low Tm may be attributed to the flexibility and low symmetry of the aliphatic carboxylate ligand, which raises the entropy of fusion (ΔSfus), and the lack of crystal field stabilization energy on metal ions, reducing enthalpy of fusion (ΔHfus). This research will serve as a cornerstone for the integration of numerous carboxylate-based MOFs into MOF glasses.
Targeting DNA2 overcomes metabolic reprogramming in multiple myeloma
Authors: Thongon Natthakan , Ma Feiyang , Baran Natalia , Lockyer Pamela , Liu Jintan , Jackson Christopher , Rose Ashley , Furudate Ken , Wildeman Bethany , Marchesini Matteo , Marchica Valentina , Storti Paola , Todaro Giannalisa , Ganan-Gomez Irene , Adema Vera , Rodriguez-Sevilla Juan Jose , Qing Yun , Ha Min Jin , Fonseca Rodrigo , Stein Caleb , Class Caleb , Tan Lin , Attanasio Sergio , Garcia-Manero Guillermo , Giuliani Nicola , Berrios Nolasco David , Santoni Andrea , Cerchione Claudio , Bueso-Ramos Carlos , Konopleva Marina , Lorenzi Philip , Takahashi Koichi , Manasanch Elisabet , Sammarelli Gabriella , Kanagal-Shamanna Rashmi , Viale Andrea , Chesi Marta , Colla Simona 
Published: February 2024

AbstractDNA damage resistance is a major barrier to effective DNA-damaging therapy in multiple myeloma (MM). To discover mechanisms through which MM cells overcome DNA damage, we investigate how MM cells become resistant to antisense oligonucleotide (ASO) therapy targeting Interleukin enhancer binding factor 2 (ILF2), a DNA damage regulator that is overexpressed in 70% of MM patients whose disease has progressed after standard therapies have failed. Here, we show that MM cells undergo adaptive metabolic rewiring to restore energy balance and promote survival in response to DNA damage activation. Using a CRISPR/Cas9 screening strategy, we identify the mitochondrial DNA repair protein DNA2, whose loss of function suppresses MM cells’ ability to overcome ILF2 ASO−induced DNA damage, as being essential to counteracting oxidative DNA damage. Our study reveals a mechanism of vulnerability of MM cells that have an increased demand for mitochondrial metabolism upon DNA damage activation.
Redox-tunable isoindigos for electrochemically mediated carbon capture
Authors: Li Xing , Zhao Xunhua , Zhang Lingyu , Mathur Anmol , Xu Yu , Fang Zhiwei , Gu Luo , Liu Yuanyue , Liu Yayuan 
Published: February 2024

AbstractEfficient CO2 separation technologies are essential for mitigating climate change. Compared to traditional thermochemical methods, electrochemically mediated carbon capture using redox-tunable sorbents emerges as a promising alternative due to its versatility and energy efficiency. However, the undesirable linear free-energy relationship between redox potential and CO2 binding affinity in existing chemistry makes it fundamentally challenging to optimise key sorbent properties independently via chemical modifications. Here, we demonstrate a design paradigm for electrochemically mediated carbon capture sorbents, which breaks the undesirable scaling relationship by leveraging intramolecular hydrogen bonding in isoindigo derivatives. The redox potentials of isoindigos can be anodically shifted by >350 mV to impart sorbents with high oxygen stability without compromising CO2 binding, culminating in a system with minimised parasitic reactions. With the synthetic space presented, our effort provides a generalisable strategy to finetune interactions between redox-active organic molecules and CO2, addressing a longstanding challenge in developing effective carbon capture methods driven by non-conventional stimuli.
The role of interfacial donor–acceptor percolation in efficient and stable all-polymer solar cells
Authors: Wang Zhen , Guo Yu , Liu Xianzhao , Shu Wenchao , Han Guangchao , Ding Kan , Mukherjee Subhrangsu , Zhang Nan , Yip Hin-Lap , Yi Yuanping , Ade Harald , Chow Philip C. Y. 
Published: February 2024

AbstractPolymerization of Y6-type acceptor molecules leads to bulk-heterojunction organic solar cells with both high power-conversion efficiency and device stability, but the underlying mechanism remains unclear. Here we show that the exciton recombination dynamics of polymerized Y6-type acceptors (Y6-PAs) strongly depends on the degree of aggregation. While the fast exciton recombination rate in aggregated Y6-PA competes with electron-hole separation at the donor–acceptor (D–A) interface, the much-suppressed exciton recombination rate in dispersed Y6-PA is sufficient to allow efficient free charge generation. Indeed, our experimental results and theoretical simulations reveal that Y6-PAs have larger miscibility with the donor polymer than Y6-type small molecular acceptors, leading to D–A percolation that effectively prevents the formation of Y6-PA aggregates at the interface. Besides enabling high charge generation efficiency, the interfacial D–A percolation also improves the thermodynamic stability of the blend morphology, as evident by the reduced device “burn-in” loss upon solar illumination.
Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages
Authors: Zhang Yinliang , Du Chunyuan , Wang Wei , Qiao Wei , Li Yuhui , Zhang Yujie , Sheng Sufang , Zhou Xuenan , Zhang Lei , Fan Heng , Yu Ying , Chen Yong , Liao Yunfei , Chen Shihong , Chang Yongsheng 
Published: February 2024

AbstractThe mechanisms underlying glucocorticoid (GC)-induced obesity are poorly understood. Macrophages are the primary targets by which GCs exert pharmacological effects and perform critical functions in adipose tissue homeostasis. Here, we show that macrophages are essential for GC-induced obesity. Dexamethasone (Dex) strongly induced Krüppel-like factor 9 (Klf9) expression in macrophages. Similar to Dex, lentivirus-mediated Klf9 overexpression inhibits M1 and M2a markers expression, causing macrophage deactivation. Furthermore, the myeloid-specific Klf9 transgene promotes obesity. Conversely, myeloid-specific Klf9-knockout (mKlf9KO) mice are lean. Moreover, myeloid Klf9 knockout largely blocks obesity induced by chronic GC treatment. Mechanistically, GC-inducible KLF9 recruits the SIN3A/HDAC complex to the promoter regions of Il6, Ptgs2, Il10, Arg1, and Chil3 to inhibit their expression, subsequently reducing thermogenesis and increasing lipid accumulation by inhibiting STAT3 signaling in adipocytes. Thus, KLF9 in macrophages integrates the beneficial anti-inflammatory and adverse metabolic effects of GCs and represents a potential target for therapeutic interventions.
The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with CXCL12
Authors: Mantonico Malisa Vittoria , De Leo Federica , Quilici Giacomo , Colley Liam Sean , De Marchis Francesco , Crippa Massimo , Mezzapelle Rosanna , Schulte Tim , Zucchelli Chiara , Pastorello Chiara , Carmeno Camilla , Caprioglio Francesca , Ricagno Stefano , Giachin Gabriele , Ghitti Michela , Bianchi Marco Emilio , Musco Giovanna 
Published: February 2024

AbstractChemokine heterodimers activate or dampen their cognate receptors during inflammation. The CXCL12 chemokine forms with the fully reduced (fr) alarmin HMGB1 a physiologically relevant heterocomplex (frHMGB1•CXCL12) that synergically promotes the inflammatory response elicited by the G-protein coupled receptor CXCR4. The molecular details of complex formation were still elusive. Here we show by an integrated structural approach that frHMGB1•CXCL12 is a fuzzy heterocomplex. Unlike previous assumptions, frHMGB1 and CXCL12 form a dynamic equimolar assembly, with structured and unstructured frHMGB1 regions recognizing the CXCL12 dimerization surface. We uncover an unexpected role of the acidic intrinsically disordered region (IDR) of HMGB1 in heterocomplex formation and its binding to CXCR4 on the cell surface. Our work shows that the interaction of frHMGB1 with CXCL12 diverges from the classical rigid heterophilic chemokines dimerization. Simultaneous interference with multiple interactions within frHMGB1•CXCL12 might offer pharmacological strategies against inflammatory conditions.
Chelation enables selectivity control in enantioconvergent Suzuki–Miyaura cross-couplings on acyclic allylic systems
Authors: Stojalnikova Violeta , Webster Stephen J. , Liu Ke , Fletcher Stephen P. 
Published: February 2024

AbstractAsymmetric Suzuki–Miyaura cross-couplings with aryl boronic acids and allylic electrophiles are a powerful method to convert racemic mixtures into enantioenriched products. Currently, enantioconvergent allylic arylations are limited to substrates that are symmetrical about the allylic unit, and the absence of strategies to control regio-, E/Z- and enantioselectivity in acyclic allylic systems is a major restriction. Here, using a system capable of either conjugate addition or allylic arylation, we have discovered the structural features and experimental conditions that allow an acyclic system to undergo chemo- and regioselective, enantioconvergent allylic Suzuki–Miyaura-type arylation. A wide variety of boronic acid coupling partners can be used, and both alkyl and aromatic substituents are tolerated on the allylic unit so that a wide variety of structures can be obtained. Preliminary mechanistic studies reveal that the chelating ability of the ester group is crucial to obtaining high regio- and enantioselectivity. Using this method, we were able to synthesize the natural products (S)-curcumene and (S)-4,7-dimethyl-1-tetralone and the clinically used antidepressant sertraline (Zoloft).
Bistability between π-diradical open-shell and closed-shell states in indeno[1,2-a]fluorene
Authors: Mishra Shantanu , Vilas-Varela Manuel , Lieske Leonard-Alexander , Ortiz Ricardo , Fatayer Shadi , Rončević Igor , Albrecht Florian , Frederiksen Thomas , Peña Diego , Gross Leo 
Published: February 2024

AbstractIndenofluorenes are non-benzenoid conjugated hydrocarbons that have received great interest owing to their unusual electronic structure and potential applications in nonlinear optics and photovoltaics. Here we report the generation of unsubstituted indeno[1,2-a]fluorene on various surfaces by the cleavage of two C–H bonds in 7,12-dihydroindeno[1,2-a]fluorene through voltage pulses applied by the tip of a combined scanning tunnelling microscope and atomic force microscope. On bilayer NaCl on Au(111), indeno[1,2-a]fluorene is in the neutral charge state, but it exhibits charge bistability between neutral and anionic states on the lower-workfunction surfaces of bilayer NaCl on Ag(111) and Cu(111). In the neutral state, indeno[1,2-a]fluorene exhibits one of two ground states: an open-shell π-diradical state, predicted to be a triplet by density functional and multireference many-body perturbation theory calculations, or a closed-shell state with a para-quinodimethane moiety in the as-indacene core. We observe switching between open- and closed-shell states of a single molecule by changing its adsorption site on NaCl.
Substantial contribution of tree canopy nitrifiers to nitrogen fluxes in European forests
Authors: Guerrieri Rossella , Cáliz Joan , Mattana Stefania , Barceló Anna , Candela Marco , Elustondo David , Fortmann Heike , Hellsten Sofie , Koenig Nils , Lindroos Antti-Jussi , Matteucci Giorgio , Merilä Päivi , Michalski Greg , Nicolas Manuel , Thimonier Anne , Turroni Silvia , Vanguelova Elena , Verstraeten Arne , Waldner Peter , Watanabe Mirai , Casamayor Emilio O. , Peñuelas Josep , Mencuccini Maurizio 
Published: February 2024

AbstractHuman activities have greatly increased the reactive nitrogen in the biosphere, thus profoundly altering global nitrogen cycling. The large increase in nitrogen deposition over the past few decades has led to eutrophication in natural ecosystems, with negative effects on forest health and biodiversity. Recent studies, however, have reported oligotrophication in forest ecosystems, constraining their capacity as carbon sinks. Here we demonstrate the widespread biological transformation of atmospheric reactive nitrogen in the canopies of European forests by combining nitrogen deposition quantification with measurements of the stable isotopes in nitrate and molecular analyses across ten forests through August–October 2016. We estimate that up to 80% of the nitrate reaching the soil via throughfall was derived from canopy nitrification, equivalent to a flux of up to 5.76 kg N ha−1 yr−1. We also document the presence of autotrophic nitrifiers on foliar surfaces throughout European forests. Canopy nitrification thus consumes deposited ammonium and increases nitrate inputs to the soil. The results of this study highlight widespread canopy nitrification in European forests and its important contribution to forest nitrogen cycling.
Abrupt Holocene ice loss due to thinning and ungrounding in the Weddell Sea Embayment
Authors: Grieman Mackenzie M. , Nehrbass-Ahles Christoph , Hoffmann Helene M. , Bauska Thomas K. , King Amy C. F. , Mulvaney Robert , Rhodes Rachael H. , Rowell Isobel F. , Thomas Elizabeth R. , Wolff Eric W. 
Published: February 2024

AbstractThe extent of grounded ice and buttressing by the Ronne Ice Shelf, which provides resistance to the outflow of ice streams, moderate West Antarctic Ice Sheet stability. During the Last Glacial Maximum, the ice sheet advanced and was grounded near the Weddell Sea continental shelf break. The timing of subsequent ice sheet retreat and the relative roles of ice shelf buttressing and grounding line changes remain unresolved. Here we use an ice core record from grounded ice at Skytrain Ice Rise to constrain the timing and speed of early Holocene ice sheet retreat. Measured δ18O and total air content suggest that the surface elevation of Skytrain Ice Rise decreased by about 450 m between 8.2 and 8.0 kyr before 1950 ce (±0.13 kyr). We attribute this elevation change to dynamic thinning due to flow changes induced by the ungrounding of ice in the area. Ice core sodium concentrations suggest that the ice front of this ungrounded ice shelf then retreated about 270 km (±30 km) from 7.7 to 7.3 kyr before 1950 ce. These centennial-scale changes demonstrate how quickly ice mass can be lost from the West Antarctic Ice Sheet due to changes in grounded ice without extensive ice shelf calving. Our findings both support and temporally constrain ice sheet models that exhibit rapid ice loss in the Weddell Sea sector in the early Holocene.
Women and insurance pricing policies: a gender-based analysis with GAMLSS on two actuarial datasets
Authors: Pernagallo Giuseppe , Punzo Antonio , Torrisi Benedetto 
Published: February 2024

AbstractIn most of the United States, insurance companies may use gender to determine car insurance rates. In addition, several studies have shown that women over the age of 25 generally pay more than men for car insurance. Then, we investigate whether the distributions of claims for women and men differ in location, scale and shape by means of the GAMLSS regression framework, using microdata provided by U.S. and Australian insurance companies, to use this evidence to support policy makers’ decisions. We also develop a parametric-bootstrap test to investigate the tail behavior of the distributions. When covariates are not considered, the distribution of claims does not appear to differ by gender. When covariates are included, the regressions provide mixed evidence for the location parameter. However, for female claimants, the spread of the distribution is lower. Our research suggests that, at least for the contexts analyzed, there is no clear statistical reason for charging higher rates to women. While providing evidence to support unisex insurance pricing policies, given the limitations represented by the use of country-specific data, this paper aims to promote further research on this topic with different datasets to corroborate our findings and draw more general conclusions.