bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methane-flow system within the Nyegga pockmark field, offshore mid-Norway
Authors: Dupré Stéphanie , Foucher Jean-Paul , Pierre Catherine , Decker Carole , Westbrook Graham , Ker Stephan , Olu Karine , Donval Jean-Pierre , Charlou Jean-Luc , Blanc-Valleron Marie-Madeleine , Nouzé Hervé 
Published: October 2023

We investigated fluid seepage within the Nyegga pockmark field (600–900 m water depths) off mid-Norway from Remotely Operated Vehicle dives at the so-called CNE sites (CNE01 to CNE17). The seafloor morphology of some of these features corresponds to pockmarks and adjacent ridges, with the latter being the focus of present seepage activity. These structures are underlain by chimneys above a gas-charged zone with, in some cases, a substantial body of hydrate-invaded sediment (down to 1.3 s in two-way travel time at CNE03). Present-day methane-rich fluid seepage through the seabed is indicated by chemosynthetic fauna, in particular Siboglinidae polychaetes (Oligobrachia haakonmobiensis webbi and Sclerolinum contortum), microbial mats and associated Rissoidae gastropod (Alvania sp.) grazers, and confirmed by measured in situ bottom-water methane anomalies, up to 2,130 nL/L. No free-gas bubble emissions were observed or acoustically identified. The presence of authigenic carbonates reveals past seepage with very low δ13C values (down to −58‰) indicating that the major source of carbon was methane carried by the venting fluids. The ages of major periods of methane venting are provided by vesicomyid bivalve shells (Isorropodon nyeggaensis) present in two sedimentary layers, 14,930 and 15,500 14C yr BP (ca. 17,238 and 17,952 cal yr BP), respectively, corresponding to the time of Melt Water Pulse IA. The seafloor morphology and pattern of seepage -chemosynthetic fauna and microbial mat distribution and dissolved methane concentration-are remarkably heterogeneous. Pore-water chemistry profiles in a gravity core taken only 40 m from major seepage sites indicate no seepage and anaerobic methane oxidation at a sub-bottom depth of about 2 m. Present-day seepage from the studied pockmark-chimney fluid-flow system charged with gas hydrate is dominated by the advection of methane solution in pore water. Some of this methane could result from the dissolution of hydrate in the chimney, most of which would have formed during an earlier period (post-LGM times) of history of the chimney, when it was venting free gas. However, the presence of free gas beneath this chimney is probably why the water entering the chimney is already saturated with methane and the process of hydrate formation in the chimney continues today.
Effects of cigarette smoking on semen quality, reproductive hormone levels, metabolic profile, zinc and sperm DNA fragmentation in men: results from a population-based study
Authors: Osadchuk Ludmila , Kleshchev Maxim , Osadchuk Alexander 
Published: October 2023

BackgroundCigarette smoking seems to have a negative impact on men’s reproductive health, but our knowledge of its effects on the reproductive function of Russian men is still very limited. The purpose of this study was to evaluate the effect of cigarette smoking on semen quality, including sperm DNA fragmentation, hormonal, zinc and metabolic status in young men from the general multi-ethnic Russian population (n=1,222, median age 23 years) and to find out the ethno-specific effects of smoking by comparing male groups of different ethnicity.MethodsEach participant filled out a standardized questionnaire, provided one blood and semen sample. Semen parameters, serum reproductive hormones, lipids, glucose, uric acid and seminal zinc were analyzed. Participants were classified as smokers (n=450) and non-smokers (n=772), and smokers were stratified into moderate (≤10 cigarettes/day) and heavy (&gt;10 cigarettes/day) smokers.ResultsIn the entire study population, heavy smokers were characterized by a decrease in semen volume, total sperm count, sperm concentration and motility, and an increase in sperm DNA fragmentation and teratozoospermia compared with non-smokers (p&lt;0.05). There was also a reduction in the serum and seminal zinc level as well as an impairment in metabolic health in smokers compared with non-smokers (p&lt;0.05). No significant differences between smokers and non-smokers were found for serum levels of LH, FSH, inhibin B, testosterone and estradiol. In the second part of our study, the most numerous ethnic groups of Slavs (n=654), Buryats (n=191), and Yakuts (n=125) were selected from the entire study population. Among three ethnic groups, the smoking intensity was higher in Slavs than in Buryats or Yakuts suggesting a greater tobacco addiction in Slavs than in Asians. A decrease in semen parameters and seminal zinc levels, and an increase in sperm DNA fragmentation and teratozoospermia was observed only in smoking Slavs (p&lt;0.05); moderate decrease in testosterone and increase in triglyceride levels were revealed in smoking Yakuts (p&lt;0.05), but no significant changes were detected in smoking Buryats.ConclusionWe concluded that cigarette smoking has an ethno-specific effect on male reproductive function, probably due to the different activity of the seminal antioxidant system, which is yet to be elucidated.
Physicochemical and adsorptive properties of biochar derived from municipal sludge: sulfamethoxazole adsorption and underlying mechanism
Authors: Qiu Xiaojie , Zhao Yingxin , Zhao Cailian , Jin Ruotong , Li Chenxi , Mutabazi Emmanuel 
Published: October 2023

Municipal sludge waste could be transformed into useful biochar through pyrolysis process. In this study, municipal sludge-derived biochar (SBC) was successfully synthesized via the one-pot pyrolysis method, and the yield of sludge biochar gradually decreased with the pyrolysis temperature increased from 300°C to 800°C. The sludge biochar exhibited an alkaline surface due to the gradual accumulation of ash and the formation of carbonate and organic anion during high-temperature pyrolysis process. Moreover, the prepared samples were analyzed by different characterization techniques including BET, SEM, and XPS. Adsorption experiments using the optimized biochar sample of SBC800 resulted in a 95% sulfamethoxazole (SMX) removal efficiency and the maximum adsorption capacity of 7033.4 mg/kg, which was 47.5 times higher than that of SBC300. The adsorption process of SBC800 for SMX was more in line with the Freundlich and D-A isotherm model, the whole process was an exothermic reaction. SBC800 could effectively remove SMX through pore filling effect, electrostatic attraction, hydrogen bonding, hydrophobic effect, and π-π EDA interaction. Site energy distribution analysis showed that SMX preferentially occupied the high-energy adsorption site of SBC800, and then gradually diffused to the low-energy adsorption site. This study proposed a sustainable method for recycling municipal sludge for organic pollutant removal.
Dependence of element composition of bile of freshwater and marine fishes on some abiotic and biotic factors
Authors: Solovyev Mikhail M. , Shokurova Anastasiya V. , Anishchenko Olesya V. , Vlasenko Pavel G. , Kashinskaya Elena N. , Andree Karl B. 
Published: October 2023

IntroductionThe elemental composition and physical properties of fish bile is a very important marker for ecotoxicological and physiological studies as bile is able to accumulate elements from environmental compartments. In the present study we focused on the effects of long term (seasons and years) and spatial distance (different water bodies) as well as other biotic (feeding habits, fish species, gut morphology) and abiotic (water salinity) factors on element composition of bile of 429 individuals belonging to 22 forms/species of fishes.MethodsElement composition was determined using an inductively coupled plasma optical emission spectrometry. The fishes were analyzed from one marine (the Kandalaksha Gulf of the White Sea), two freshwater oligotrophic (Teletskoye and Baunt lakes), and one freshwater eutrophic water body (Chany Lake). In parallel, we have estimated the volume and density of bile from the same fishes using an automatic pipette and electronic balance.Results and discussionBased on fish bile density and volume data, we hypothesize that gastric fish species (those fishes that have a stomach) require a low volume of bile, but with higher density if compared to agastric fish species (those lacking a stomach) that mainly possessed large volumes of bile, but with a lower density. We have found that the concentrations of the following Na, K, Ca, Mg, S, P (major elements) and Al, Cu, Fe, Sr, Zn (trace elements) were the highest among the 28 elements studied. There were significant findings to contribute to a better understanding of the physiology of bile. First, we have determined the conditions formed in a water body in a given year (season) are more important for element composition of fish bile rather than other tested factors (feeding habits, gut morphology, etc.). Second, the feeding habits of fishes (carnivorous compared to non-carnivorous) had a significant effect based on differences among several elements. Third, the clear distinction between marine and freshwater fishes was found only for K and Na. Understanding which elements are produced as part of waste elimination and those that are present as a consequence of vital biological functions could improve the ecotoxicological study of bile as a marker of heavy metal contamination.
Effect of cyclic substituents on the anti-cancer activity and DNA interaction of ruthenium(II) bis-phenanthroline dipyridoquinoline
Authors: Nyong-Bassey Etubonesi E. , Hicks Andrew L. , Bergin Poppy , Tuite Eimer M. , Kozhevnikov Valery , Veuger Stephany 
Published: October 2023

Introduction: Ruthenium(II) complexes have emerged recently as candidates for anti-cancer therapy, where activity is related to lipohilicity, cellular localization, and specific interactions with biomolecules.Methods: In this work, two novel complexes were synthesized and are reported based on the [Ru(phen)2(dipyrido[3,2-f:2′,3′-h]quinoxaline]2+ framework.Results: Compared to the parent complex, annealing of cyclopenteno and cyclohexeno rings to the extended ligand substantially increased cytotoxicity towards a number of cancer cell lines, and induced apoptosis. The complexes localize in the nuclei of cancer cells and co-locate with DAPI on DNA. DNA binding studies show that both complexes bind strongly to DNA and one complex intercalates DNA like the parent, whilst the other appears to have multiple modes of interaction.Discussion: It is likely that the increased lipophilicity of the novel complexes is a key factor for increasing their cytotoxicity, rather than their DNA binding mode.
Research trends and hotspots regarding treatment of obstructive sleep apnea
Authors: Yang Xia , Wen Yi , Xie Shiqi , Chen Jinglan , Liu Yue , Zhou Jianrong 
Published: October 2023

BackgroundObstructive sleep apnea (OSA) is a type of sleep-disordered breathing disease, with high prevalence and multiple complications. It seriously affects patients’ quality of life and even threatens their lives. Early and effective treatment can significantly improve patients’ health conditions.ObjectiveIn this study, the main treatment methods, research hotspots and trends of OSA were summarized through bibliometric and visualization analysis.MethodsFrom the Web of Science Core Collection database, articles on the treatment of OSA from 1999 to 2022 were obtained. CiteSpace and VOSviewer were comprehensively used to visualization of journals, co-authorship of countries, institutions and authors, co-citation of references, keywords cluster and burst.ResultsA total of 2,874 publications were obtained, of which 2,584 were concerned adults and 290 about children. In adults’ research, Sleep and Breathing is the most published journal (280, 10.84%), the largest number of publications come from the United States (636,24.61%) and the University of Sydney (88, 3.41%), and Pepin JL is the most published author (48, 18.58%). In children’s studies, International Journal of Pediatric Otorhinolaryngology is the most published journal (41, 14.14%), the maximum number of publications were also from the United States (123, 42.41%), with the University of Pennsylvania (20, 6.90%) and Marcus CL (15, 5.17%) being the most published institutions and authors. High-frequency keywords for adults’ researches include positive airway pressure, oral appliance, surgery and positional therapy. On these basis, children’s studies also focus on myofunctional therapy, rapid maxillary expansion and hypoglossal nerve Stimulation.ConclusionOver the past two decades, research in the field of OSA therapeutics has experienced significant growth in depth and breadth. The author cooperation network has already established a solid foundation, while there is potential for further strengthening the cooperation network between countries and institutions. Currently, positive airway pressure and surgery are the primary treatments for OSA in adults and children. Future research will focus on multidisciplinary combination targeted therapy, which presents a key area of interest and challenge.
Dietary and lifestyle oxidative balance scores are independently and jointly associated with nonalcoholic fatty liver disease: a 20 years nationally representative cross-sectional study
Authors: Liu Yuanbin , Chen Mingkai 
Published: October 2023

BackgroundOxidative stress is an important contributor to the progression of nonalcoholic fatty liver disease (NAFLD), but whether dietary and lifestyle pro- and antioxidants may have combined or independent effects on NAFLD, and advanced liver fibrosis (AHF) remains unclear. We aimed to elucidate the relationship between a well-established oxidative balance score (OBS) and NAFLD/AHF.MethodsThis was a cross-sectional study. We included adult participants with complete data from the National Health and Nutrition Examination Survey 1999–2018. Survey-weighted adjusted multivariate regression analyses were used to examine the association of all OBS with NAFLD/AHF. A combination of restricted cubic splines, mediation analysis, stratified analysis, and sensitivity analysis were used to further elucidate these associations.ResultsWe included 6,341 eligible adult participants with prevalence of NAFLD and AHF of 30.2 and 13.9%, respectively. In the fully adjusted model, the highest quartile of OBS, dietary OBS, and lifestyle OBS were associated with 65, 55, and 77% reduced risk of NAFLD, respectively, compared with the reference population, respectively. However, all OBS were not associated with the risk of AHF. All OBS were nonlinearly associated with risk of NAFLD and had a more pronounced reduced risk for OBS, dietary OBS, and lifestyle OBS after exceeding 26, 21, and 5 points, respectively. OBS may exert a protective effect indirectly through inflammation, oxidative stress, and glycolipid metabolism markers. Stratification and sensitivity analyses demonstrate the robustness of our findings.ConclusionAll OBS were nonlinearly and negatively associated with NAFLD risk. These effects may exert indirectly through inflammation, oxidative stress, and glycolipid metabolism markers.
Preferences for the sequencing of first-line systemic treatments in metastatic hormone receptor-positive, HER2-negative breast cancer
Authors: Al Mahmasani Layal , Amhaz Ghid , Abou Zeidane Reine , Chamseddine Nathalie , Hatab Taha , Sabbagh Saad , Charafeddine Maya , Assi Hazem I. 
Published: October 2023

IntroductionMetastatic breast cancer (MBC) is a diverse disease. Therapeutic options include hormonal therapy, chemotherapy, and targeted therapies. The optimal treatment sequence for patients with hormone receptor-positive (HR-positive), HER2-negative metastatic breast cancer remains unknown.MethodsThis was a retrospective and prospective study. The data was collected from the medical records of patients in a tertiary healthcare center in Lebanon between the years 2016 and 2019, and patients were followed up for a 3-year duration. The main aim was to identify oncologists’ preferences in the choice and sequence of treatment for newly diagnosed and/or recurrent cases of HR-positive, HER2-negative MBC.ResultsA total of 51 patients were included. 24 patients received chemotherapy, while 27 received endocrine therapy as first-line treatment after a diagnosis of MBC, with a median overall survival (OS) of 13 months and a median progression-free survival (PFS) of 12 months after first-line treatment with chemotherapy, compared to 27 months and 18 months with endocrine therapy. A higher percentage of patients have received chemotherapy in the first-line setting compared to the data reported in the literature, with the choice being multifactorial.ConclusionFactors to consider in MBC management include the choice of first-line treatment, the optimal sequence of treatment, and the combination of available treatment options.
Risk factors for health impairments in children after hospitalization for acute COVID-19 or MIS-C
Authors: Maddux Aline B. , Young Cameron C. , Kucukak Suden , Zambrano Laura D. , Newhams Margaret M. , Rollins Caitlin K. , Halasa Natasha B. , Gertz Shira J. , Mack Elizabeth H. , Schwartz Stephanie , Kong Michele , Loftis Laura L. , Irby Katherine , Rowan Courtney M. , Tarquinio Keiko M. , Zinter Matt S. , Crandall Hillary , Cvijanovich Natalie Z. , Schuster Jennifer E. , Fitzgerald Julie C. , Staat Mary A. , Hobbs Charlotte V. , Nofziger Ryan A. , Shein Steven , Flori Heidi , Cullimore Melissa L. , Chatani Brandon M. , Levy Emily R. , Typpo Katri V. , Hume Janet R. , Campbell Angela P. , Randolph Adrienne G. ,   the Overcoming COVID-19 Investigators
Published: October 2023

ObjectiveTo identify risk factors for persistent impairments after pediatric hospitalization for acute coronavirus disease 2019 (COVID-19) or multisystem inflammatory syndrome in children (MIS-C) during the SARS-CoV-2 pandemic.MethodsAcross 25 U.S. Overcoming COVID-19 Network hospitals, we conducted a prospective cohort study of patients &lt;21-years-old hospitalized for acute COVID-19 or MIS-C (May 2020 to March 2022) surveyed 2- to 4-months post-admission. Multivariable regression was used to calculate adjusted risk ratios (aRR) and 95% confidence intervals (CI).ResultsOf 232 children with acute COVID-19, 71 (30.6%) had persistent symptoms and 50 (21.6%) had activity impairments at follow-up; for MIS-C (n= 241), 56 (23.2%) had persistent symptoms and 58 (24.1%) had activity impairments. In adjusted analyses of patients with acute COVID-19, receipt of mechanical ventilation was associated with persistent symptoms [aRR 1.83 (95% CI: 1.07, 3.13)] whereas obesity [aRR 2.18 (95% CI: 1.05, 4.51)] and greater organ system involvement [aRR 1.35 (95% CI: 1.13, 1.61)] were associated with activity impairment. For patients with MIS-C, having a pre-existing respiratory condition was associated with persistent symptoms [aRR 3.04 (95% CI: 1.70, 5.41)] whereas obesity [aRR 1.86 (95% CI: 1.09, 3.15)] and greater organ system involvement [aRR 1.26 (1.00, 1.58)] were associated with activity impairments.DiscussionAmong patients hospitalized, nearly one in three hospitalized with acute COVID-19 and one in four hospitalized with MIS-C had persistent impairments for ≥2 months post-hospitalization. Persistent impairments were associated with more severe illness and underlying health conditions, identifying populations to target for follow-up.
Case report: Extrapontine myelinolysis combined with flupentixol- and melitracen-induced dysphagia
Authors: Zhao Zhihong , Han Lu , Zhu Zilong 
Published: October 2023

Extrapontine myelinolysis (EPM) is a rare symmetrical demyelinating disease of the central nervous system, which is often accompanied with central pontine myelinolysis (CPM) or can appear alone. A combination of flupentixol and melitracen is used as an antianxiety–antidepressant drug which may induce hyponatremia. Herein, we report a 46-year-old woman with depression who was treated with flupentixol and melitracen 0.5/10 mg once daily for 6 months. Later, the dosage increased to 0.5/10 mg twice daily. At the same time, she had complains of intermittent dizziness and fatigue. The laboratory test revealed hyponatremia (121 mmol/L). Dizziness was improved after sodium supplementation, with an increase in blood sodium to 133 mmol/L. Twenty days later, she had difficulty opening the mouth and swallowing, needing a gastric tube due to severe dysphagia. Head magnetic resonance imaging (MRI) showed a symmetric abnormal signal of caudate nucleus and lenticular nuclei. The symptoms were not relieved after active treatment, such as rehydration. However, her symptoms improved significantly after discontinuation of flupentixol and melitracen and switching to promethazine. Follow-up head MRI after 4 months revealed no abnormal signals. The patient who developed EPM had dysphagia, despite appropriate correction of hyponatremia. Flupentixol and melitracen can cause hyponatremia and dysphagia. This case highlights an unexpected association between EPM and flupentixol- and melitracen-induced dysphagia.
The Nociceptin/Orphanin FQ peptide receptor antagonist, SB-612111, improves cerebral blood flow in a rat model of traumatic brain injury
Authors: Al Yacoub Omar N. , Tarantini Stefano , Zhang Yong , Csiszar Anna , Standifer Kelly M. 
Published: October 2023

Traumatic brain injury (TBI) affects more than 2.5 million people in the U.S. each year and is the leading cause of death and disability in children and adults ages 1 to 44. Approximately 90% of TBI cases are classified as mild but may still lead to acute detrimental effects such as impaired cerebral blood flow (CBF) that result in prolonged impacts on brain function and quality of life in up to 15% of patients. We previously reported that nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor antagonism reversed mild blast TBI-induced vestibulomotor deficits and prevented hypoxia. To explore mechanisms by which the NOP receptor-N/OFQ pathway modulates hypoxia and other TBI sequelae, the ability of the NOP antagonist, SB-612111 (SB), to reverse TBI-induced CBF and associated injury marker changes were tested in this study. Male Wistar rats randomly received sham craniotomy or craniotomy + TBI via controlled cortical impact. Injury severity was assessed after 1 h (modified neurological severity score (mNSS). Changes in CBF were assessed 2 h post-injury above the exposed cortex using laser speckle contrast imaging in response to the direct application of increasing concentrations of vehicle or SB (1, 10, and 100 µM) to the brain surface. TBI increased mNSS scores compared to baseline and confirmed mild TBI (mTBI) severity. CBF was significantly impaired on the ipsilateral side of the brain following mTBI, compared to contralateral side and to sham rats. SB dose-dependently improved CBF on the ipsilateral side after mTBI compared to SB effects on the respective ipsilateral side of sham rats but had no effect on contralateral CBF or in uninjured rats. N/OFQ levels increased in the cerebral spinal fluid (CSF) following mTBI, which correlated with the percent decrease in ipsilateral CBF. TBI also activated ERK and cofilin within 3 h post-TBI; ERK activation correlated with increased CSF N/OFQ. In conclusion, this study reveals a significant contribution of the N/OFQ-NOP receptor system to TBI-induced dysregulation of cerebral vasculature and suggests that the NOP receptor should be considered as a potential therapeutic target for TBI.