bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimal mechanical stretch promotes TSP-1 expression through Akt and GSK-3β/β-catenin signaling pathways in keloid formation.
Authors: Xu Xiangwen , Ou Yanting , Zhang Yihan , Kang Yixing , Wu Mengfan , Feng Jun , Long Yun , Cui Yongyan , Liu Dandan , Luo Lin 
Published: October 2023

Abstract
Background:
The formation of keloids is influenced by various factors, with local mechanical stretch being recognized as a significant contributing component. Thrombospondin-1 (TSP-1) is identified as a tension-sensitive protein. However, the relationship between TSP-1 and keloid formation induced by mechanical stretch remains unknown.
Materials and Methods:
A simple customized mechanical stretch device was used for the application of homogeneous equibiaxial stretch (HES). Using Western blot and RT-PCR, the optimal stretch strength and duration were determined. Regarding the functional changes induced by stretch in keloid fibroblasts (Kfbs), cell function assays were used. The relationship between TSP-1 expression and stretch-induced scar formation in human and animal models was investigated using immunohistochemistry. The knock-down of TSP-1 in fibroblasts served as a reverse test. The potential signaling pathway, namely Akt and GSK-3β/β-catenin, were evaluated using western blot.
Results:
Optimal HES (oHES) could be achieved with four rotations of the screws on our mechanical stretch device, resulting in a significant increase in vimentin, Col I, and fibronectin expression in Kfbs on day 5. Additionally, oHES significantly promoted cell proliferation and migration, but not apoptosis. oHES resulted in the upregulation of TSP-1 expression in both in vitro and in vivo experimental settings. By knocking down TSP-1, the expression of proteins, particularly Col I, and the oHES-induced alterations in the cellular function of Kfbs could be significantly reduced. The inhibition of TSP-1 may attenuate pHES-induced keloid formation through the Akt and GSK-3β/β-catenin signaling pathways.
Conclusions:
The results confirmed that oHES could enhance the proliferative and migratory functions of Kfbs by increasing the expression of Col I, fibronectin, and vimentin. oHES promoted keloid formation by increasing Col I expression through TSP-1-mediated Akt and GSK-3β/β-catenin signaling pathways.
Myoelectric interface for neurorehabilitation conditioning to reduce abnormal leg co-activation after stroke: a pilot study
Authors: Khorasani Abed , Hulsizer Joel , Paul Vivek , Gorski Cynthia , Dhaher Yasin Y. , Slutzky Marc W. 
Published: October 2023

Abstract
Background
The ability to walk is an important factor in quality of life after stroke. Co-activation of hip adductors and knee extensors has been shown to correlate with gait impairment. We have shown previously that training with a myoelectric interface for neurorehabilitation (MINT) can reduce abnormal muscle co-activation in the arms of stroke survivors.
Methods
Here, we extend MINT conditioning to stroke survivors with leg impairment. The aim of this pilot study was to assess the safety and feasibility of using MINT to reduce abnormal co-activation between hip adductors and knee extensors and assess any effects on gait. Nine stroke survivors with moderate to severe gait impairment received six hours of MINT conditioning over six sessions, either in the laboratory or at home.
Results
MINT participants completed a mean of 159 repetitions per session without any adverse events. Further, participants learned to isolate their muscles effectively, resulting in a mean reduction of co-activation of 70% compared to baseline. Moreover, gait speed increased by a mean of 0.15 m/s, more than the minimum clinically important difference. Knee flexion angle increased substantially, and hip circumduction decreased.
Conclusion
MINT conditioning is safe, feasible at home, and enables reduction of co-activation in the leg. Further investigation of MINT’s potential to improve leg movement and function after stroke is warranted. Abnormal co-activation of hip adductors and knee extensors may contribute to impaired gait after stroke.
Trial registration
This study was registered at ClinicalTrials.gov (NCT03401762, Registered 15 January 2018, https://clinicaltrials.gov/study/NCT03401762?tab=history&a=4)
Shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements
Authors: Fouquet Vincent , Dantagnan Claire-Adeline , Abdel-Gawad Sarah , Dursun Elisabeth , Attal Jean-Pierre , François Philippe 
Published: October 2023

Abstract
Objective:Evaluation of the Shear bond strength over zirconia and titanium alloy and degree of conversion of extraoral compared to intraoral self-adhesive resin cements.
Materials and methods: Nine bonding protocols were carried out on zirconia 4Y-TZP and titanium alloy grade V. Seven resin cement were tested in the shear bond strength test and the degree of conversion measurements. One extraoral (Multilink hybrid abutment) and six intraoral (G-cem One, SpeedCem Plus, RelyX Universal, Panavia SA Universal, Nexus Universal, Totalcem).
Results: The significantly highest value was obtained for Monobond Plus + Multilink Hybrid Abutment, the extraoral resin cement for both titanium alloy and zirconia. For each resin, significantly higher DC values were obtained for the dual-cure mode compared with the self-cure mode. Regardless of the cure mode, Nexus Universal reached the highest DC.
Discussion/Conclusions: In this study, the extraoral self-curing resin cement showed the best bond strength values on zirconia and titanium alloy when associated with a universal primer. Some intraoral dual-cure resin cements showed closed performances when used with universal primers. There is no direct correlation between the degree of conversion of the resin cement and the shear bond strength obtained on the prosthetic materials tested.
Psychache Status and Associated Contributing Factors among the Hakka Elderly in Fujian, China
Authors: Chen Yating , Cai Longhua , Ruan Wenqian , Zhang Lingling , Liu Xiaojun 
Published: October 2023

Abstract

Background:Chinese elderly are more psychologically fragile and prone to mental illness than young people. This study investigates psychache and associated factors among Hakka elderly in Fujian, China.
Methods: The Chinese version of the Psychache Scale (PAS) was used to assess the frequency and intensity of psychache in Hakka older adults. Multilevel linear regression analysis was conducted to identify the main socio-demographic factors associated with psychache overall and its frequency and intensity.
Results: A total of 1,262 older adults participated, with mean scores of 18.27 ± 6.88 for total PAS, 12.50 ± 4.79 for PAS-Frequency and 5.77 ± 2.34 for PAS-Intensity. On average, females scored higher than males on PAS-Frequency (β = 0.84, 95% CI = 0.34, 1.35) and PAS-Intensity (β = 0.32, 95% CI = 0.07, 0.58). Older adults currently living in towns (β = -2.18, 95% CI = -2.81, -1.54), with their spouse only (β = -3.71, 95% CI = -4.77, -2.65), or with children (β = -3.24, 95% CI = -4.26, -2.22) were more likely to score lower on PAS-Frequency. Conversely, older adults who were regular sleepers (β = -1.19, 95% CI=-1.49, -0.88 ) or lived with their spouse only (β = -1.25, 95% CI = -1.78, -0.72) were more likely to score lower on PAS-Intensity.
Conclusion: Among Hakka elderly, we found a higher frequency and greater intensity of psychache in females, those with poor health status, irregular sleepers, rural residents, solo dwellers, those with below CNY 10,000 in personal savings, and the medically uninsured. The study’s findings indicate that policymakers should give more attention to the susceptible population and implement practical interventions to reduce their psychological burden.
Time-penalized trees (TpT): a new tree-based data mining algorithm for time-varying covariates
Authors: Valla Mathias 
Published: October 2023

Abstract

This article proposes a new decision tree algorithm that accounts for time-varying covari-ates in the decision-making process. Traditional decision tree algorithms assume that the covariates are static and do not change over time, which can lead to inaccurate predictions in dynamic environments. Other existing methods suggest workaround solutions such as the pseudo-subject approach, discussed in the article. The proposed algorithm utilizes a different structure and a time-penalized splitting criterion that allow a recursive partitioning of both the covariates space and time. Relevant historical trends are then inherently involved in the construction of a tree, and are visible and interpretable once it is fit. This approach allows for innovative and highly interpretable analysis in settings where the covariates are subject to change over time. The effectiveness of the algorithm is demonstrated through real-world data analysis, highlighting its potential applications in various fields, including healthcare, finance, insurance, environmental monitoring, and data mining in general.
Mesenchymal stem cells-derived extracellular vesicles protect against oxidative stress- induced xenogeneic biological root injury via adaptive regulation of the PI3K/Akt/NRF2 pathway
Authors: Fu Haojie , Sen Lin , Zhang Fangqi , Liu Sirui , Mi Hongyan , Wang Meiyue , Li Bingyan , Peng Shumin , Hu Zelong , Sun Jingjing , Li Rui 
Published: October 2023

Abstract
Xenogeneic extracellular matrices (xECM) for cell support have emerged as a potential strategy for addressing the scarcity of donor matrices for allotransplantation. However, the poor survival rate or failure of xECM-based organ transplantation is due to the negative impacts of high-level oxidative stress and inflammation on seed cell viability and stemness. Herein, we constructed xenogeneic bioengineered tooth roots (bio-roots) and used extracellular vesicles from human adipose-derived mesenchymal stem cells (hASC-EVs) to shield bio-roots from oxidative damage. Pretreatment with hASC-EVs reduced cell apoptosis, reactive oxygen species generation, mitochondrial changes, and DNA damage. Furthermore, hASC-EV treatment improved cell proliferation, antioxidant capacity, and odontogenic and osteogenic differentiation, while significantly suppressing oxidative damage by activating the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and nuclear factor erythroid 2 (NFE2)-related factor 2 (NRF2) nuclear translocation via p62-associated Kelch-like ECH-associated protein 1 (KEAP1) degradation. Inhibition of PI3K/Akt and Nrf2 knockdown reduced antioxidant capacity, indicating that the PI3K/Akt/NRF2 pathway partly mediates these effects. In subcutaneous grafting experiments using Sprague–Dawley rats, hASC-EV administration significantly enhanced the antioxidant effect of the bio-root, improved the regeneration efficiency of periodontal ligament-like tissue, and maximized xenograft function. Conclusively, Therefore, hASC-EVs have the potential to be used as an immune modulator and antioxidant for treating oxidative stress-induced bio-root resorption and degradation, which may be utilized for the generation and restoration of other intricate tissues and organs.
Methane-Rich Saline Ameliorates Depressive-Like Behaviors During Chronic Unpredictable Mild Stress (CUMS)
Authors: nasab Mohammad ghaffari , Rezvani Mohammad Ebrahim , Hosseini Mohammad Sadeghe , Mehrjerdi Fatemeh Zare 
Published: October 2023

Abstract
Depression is considered the most prevalent neuropsychiatric disorder, which is also a multifactorial and complex disease. Oxidative stress and inflammation are essential contributors to the occurrence and progression of depression. On the other hand, methane is a new therapeutic gas that has been effective in increasing tissue resistance against ischemic injuries and inflammation, so this study was conducted to investigate the effect of methane-rich saline (MRS) on depression. The chronic unpredictable mild stress (CUMS) model was designed with daily mild stressors for two months. MRS was used to treat depressed rats. Depression-like behaviors were assessed with sucrose preference, open field, and forced swimming tests. Besides, the cognitive performance of the animals was determined by the Morris water maze. Serum corticosterone levels, the activity of antioxidant enzymes, and hippocampal malondialdehyde (MDA) levels were measured, and histological changes in the hippocampus were also investigated. The results indicated that MRS decreased depressive-like behaviors and cognitive impairment in depressed animals by increasing the ratio of sucrose consumption, elevating exploratory behaviors in the open field test, reducing immobility in the forced swimming test, and improving memory and learning in the Morris water maze. Additionally, the administration of MRS regulated the corticosterone level in the serum. The level of hippocampal lipid peroxidation and tissue damage was also reduced in treated animals. The results of the present study show that MRS reduces tissue damage and lipid peroxidation in the hippocampus of CUMS animals, which is followed by a significant improvement in behavioral and cognitive functions. Probably, MRS affects by reducing oxidative stress and improving the hypothalamus-pituitary-adrenal axis function.
Cost effectiveness analysis of Uterine botulinum toxin injections versus conventional treatment in severe dysmenorrhoea
Authors: Kouame Jean Martial , Bautrant Éric , Levêque Christine , Guertin Jason Robert , Santos Mélina , Delorme Jessica , Franké Oona , Amiel Christophe , Bensousan Thierry , Thiers-Bautrant Dominique , Siani Carole 
Published: October 2023

Abstract
Objectives:
To evaluate the efficiency of botulinum toxin (BT) for the management of severe dysmenorrhoea, after failure of conventional treatments (CT) (hormonal treatments + analgesics) by an incremental cost-effectiveness ratio (ICER), from the perspective of the French Health Insurance (HI).
Methods:
This was a retrospective study (Before and After comparison) based on the patients' medical records (n=20). Data on health care consumption and quality of life were collected prospectively during the Before phase, which corresponds to the CT period, and the After phase, which corresponds to the period after the addition of BT injection. The data were analysed over a time horizon of one year, according to the perspective of the French HI. In the main analysis, total average costs included direct and indirect costs. Efficiency was assessed using the incremental cost-effectiveness ratio (ICER). The innovative strategy was considered efficient at a threshold of 30,000 € / QALY. A probabilistic sensitivity analysis using the Monte Carlo method was performed to take into account the uncertainty around the ICER related to sampling fluctuations, as well as a deterministic sensitivity analysis to evaluate the sensitivity of the ICER to the model hypotheses.
Results:
The results of the main analysis indicate, from the perspective of the French HI system, that the combination of TB + CT with an ICER: -981.98€ / QALY gained [-2187.48 ; 897.46], was the most efficient strategy at the 30,000€ efficiency threshold (dominant strategy). The combination of BT + CT: 714.82€ +/- 336.43€ was less costly than CT alone: 1104.16€ +/- 227.37€. The main cost item in our study was the cost of daily allowances. In addition, BT was more efficient than CT in terms of QALYs gained. Therefore, the use of BT in addition to CT is an efficient and beneficial strategy that could be considered for the management of dysmenorrheic patients.
Conclusion:
This study showed that, for patients with severe dysmenorrhea who are not adequately managed with CT alone, BT + CT appeared to be clinically effective and cost effective in the perspective of the French HI.
Implementation of a robotic surgical practice in inflammatory bowel disease
Authors: Ferrari Ms Linda , Nikolaou Ms Stella , Adams Ms Katie 
Published: October 2023

Abstract

Background
Robotics adoption has increased in colorectal surgery. While there are well-established advantages and standardised techniques for cancer patients, the use of robotic surgery in inflammatory bowel disease (IBD) hasn’t been studied yet.
Objective
To evaluate the feasibility and safety of robotic surgery for IBD patients.
Methods
Prospectively data in IBD patients having robotic resection at Guy’s and St Thomas’ hospital. All resections performed by a single colorectal surgeon specialised in IBD, utilising Da-Vinci platform.
Results
July 2021 to January 2023, 59 robotic IBD cases performed, 14 ulcerative colitis (UC) and 45 Crohn’s disease (CD). Average age; CD patients 35, UC 33 years. Average Body mass index (BMI); 23 for CD and 26.9 for UC patients.
In total, we performed 31 ileo-caecal resections (ICR) with primary anastomosis (18 Kono-S anastomosis, 6 mechanical anastomosis and 7 ileo-colostomy), of those 4 had multivisceral resections (large bowel, bladder, ovary). Furthermore, 14 subtotal colectomy (1 emergency), 8 proctectomy, 3 panproctocolectomy and 3 ileo-anal J pouch. 18 of the 45 patients (45.0%) with crohn’s disease had ongoing fistulating disease to other parts of the GI tract (small or large bowel). ICR were performed using different 3 ports position, depending on the anatomy established prior to surgery with magnetic resonance images (MRI). One patient had conversion to open due to anaesthetic problems and one patient required re-operation to refashion stoma. 98.0% cases completed robotically. Median Length of hospital stay (LOS) was 7 days for CD and 7 for UC cases, including LOS in patients on pre-operative parenteral nutrition.
Conclusions
Robotic colorectal techniques can be safely used for patients with IBD, even with fistulating disease. Future research and collaborations are necessary to standardize technique within institutions.
Shyness and adolescents’ smartphone addiction: Roles of maladaptive cognitive emotion regulation, experiential avoidance, and online social support
Authors: Gao Ling , Kong Fangyuan , Liu Fengfeng , Wang Xingchao 
Published: October 2023

Abstract
Although previous research has demonstrated shyness plays a critical role in the development and maintenance of smartphone addiction, the mediating and moderating mechanisms underlying this relationship remains obscure. The present study aimed to examine the mediating roles of maladaptive cognitive emotion regulation strategies and experiential avoidance and the moderating role of online social support in the relationship between shyness and adolescents’ smartphone addiction. A sample of 513 Chinese adolescents (Mage = 16.16 years, range from 14 to 19) was recruited to complete the questionnaires. After sex and age were controlled, shyness was significantly and positively associated with adolescents’ smartphone addiction and this relationship was partially mediated by both maladaptive cognitive emotion regulation strategies and experiential avoidance. The multiple mediation analysis further indicated that maladaptive cognitive emotion regulation strategies and experiential avoidance exerted a mixed mediation effect on the relationship between shyness and adolescents’ smartphone addiction. Moreover, online social support significantly moderated the relationship between shyness and smartphone addiction and this relationship is much weaker for adolescents with high online social support. These results contribute to design the effective interventions of adolescents’ smartphone addiction.
Histology and morphometry of the skin of purple spaghetti-eel Moringua raitaborua (Anguilliformes, Moringuidae)
Authors: Kim Hyun-Tae 
Published: October 2023

Abstract
The purple spaghetti-eel Moringua raitaborua lives on the sandy or muddy bottoms of estuaries, which are subject to rapid and wide changes in salinity, pH, and osmoregulatory and hypoxic conditions due to the influx of organic materials from sources of freshwater. The species has adapted to hypoxic environments by developing a thicker epidermis with stratified polygonal cells, club cells, two types of mucous cells (goblet and, oval/stratified/cuboidal cells) and dermis with blood capillaries. Among them, histological modification of thinner dorsal, lateral, and ventral body skin to include abundant capillaries and well-developed dermal vascularization may provide cutaneous respiration, permitting survival in brackish waters with low levels of oxygen and variable environmental parameters.
Parental mental health and reporting of their child’s behaviour: measurement invariance of the French version of the parental Strengths and Difficulties Questionnaire
Authors: SAPIN Arnaud , VANIER Antoine , DESCARPENTRY Arthur , MAFFRE-MAVIEL Gustave , VUILLERMOZ Cécile , FALISSARD Bruno , GALERA Cédric , WARSZAWSKI Josiane , DAVISSE-PATURET Camille , HAZO Jean-Baptiste , ROUQUETTE Alexandra 
Published: October 2023

Abstract
Symptomatic effects of mental disorders in parents could bias their reporting on their child’s mental health. This study aimed to investigate the measurement invariance of the French version of the parental Strengths and Difficulties Questionnaire (SDQ) across parental mental health in a sample (N=20,765) of parents of children aged 3 to 17 years in France. Confirmatory Factor Analysis (CFA) and Exploratory Structural Equation Modelling (ESEM) were used to evaluate the fit of three known alternative SDQ factor structures (five, three, or second-order factor structures). Invariance was tested across parental mental health (present anxiety and depressive symptoms, psychiatric history) and across socio-demographic characteristics (child's age, child's gender, parent's gender, parent's educational level). CFA models showed a poor fit, while all ESEM models achieved acceptable or good fit, with the five-factor model presenting the best fit. Invariance was observed for all characteristics tested, indicating that the SDQ can be used to study the links between parental mental health and their child's mental health without bias. However, ESEM showed that the Hyperactivity/Inattention and Conduct Problems dimensions were not well differentiated in the French version of the SDQ.
Peripherally tetra-substituted zinc (II) phthalocyanine sensitized TiO 2 composite: Monitoring with tandem LC/MS and photocatalytic degradation of amoxicillin
Authors: Keşir Melek Koç , Bıyıklıoğlu Zekeriya 
Published: October 2023

Abstract
The presence of pharmaceutically active compounds, including antibiotics, in various waters is of concern worldwide. The global handicap is that antibiotics derivatives, even when present in low concentrations in the environment, can lead to the development of antibiotic-resistant bacteria. The main purpose of this research was to degrade amoxicillin (AMX), which rapidly transforms into various degradation products (DPs) that can prove resistant to removal from water sources. To this end, the use of TiO2 nanocomposites with modified zinc phthalocyanine (ZnPc)/TiO2 was considered to both degrade the AMX compound and remove its possible DPs. The (ZnPc)/TiO2 nanocomposites are firstly synthesized by sol-gel method using titanium isopropoxide and ZnPc as Ti source, and dye pigments, respectively. The (ZnPc)/TiO2 samples were characterized by scanning electron microscopy / energy dispersive X-ray analyzer (SEM/EDX), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and Ultraviolet–Visible Diffuse Reflectance Spectroscopy (UV-DRS). To estimate the DPs formed during the photocatalytic cleavage of AMX, a triple quadrupole LC/MS/MS (Tandem MS2) was performed. It was proposed possible DPs empirical structures as structural by paying attention to the literature information in identifying possible DPs. Mineralization of AMX was also investigated by total organic carbon (TOC) analysis, suggesting that either amoxicillin itself or its resistant metabolites are still in solution and mineralization did not occur in the an expected manner.