bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica
Authors: Jin Hyunbin , Kim Sojeong , Lee Daeyeol , Ledesma-Amaro Rodrigo , Hahn Ji-Sook 
Published: October 2023

Abstract
Background
Mycosporine-like amino acids (MAAs), including shinorine and porphyra-334, are gaining attention as safe natural sunscreens. The production of MAAs has been achieved in diverse microbial hosts, including Saccharomyces cerevisiae. While S. cerevisiae is the most extensively studied model yeast, the oleaginous yeast Yarrowia lipolytica has emerged as a promising candidate for the synthesis of valuable products. In this study, we explored the potential of Y. lipolytica as a host for producing MAAs, utilizing its advantages such as a robust pentose phosphate pathway flux and versatile carbon source utilization.

Results
We produced MAAs in Y. lipolytica by introducing the MAA biosynthetic genes from cyanobacteria Nostoc punctiforme and Anabaena variabilis. These genes include mysA, mysB, and mysC responsible for producing mycosporine-glycine (MG) from sedoheptulose 7-phosphate (S7P). The two strains utilize different enzymes, D-Ala-D-Ala ligase homologue (MysD) in N. punctiforme and NRPS-like enzyme (MysE) in A. variabilis, for amino acid conjugation to MG. MysE specifically generated shinorine, a serine conjugate of MG, while MysD exhibited substrate promiscuity, yielding both shinorine and a small amount of porphyra-334, a threonine conjugate of MG. We enhanced MAAs production by selecting mysA, mysB, and mysC from A. variabilis and mysD from N. punctiforme based on their activities. We further improved production by strengthening promoters, increasing gene copies, and introducing the xylose utilization pathway. Co-utilization of xylose with glucose or glycerol increased MAAs production by boosting the S7P pool through the pentose phosphate pathway. Overexpressing GND1 and ZWF1, key genes in the pentose phosphate pathway, further enhanced MAAs production. The highest achieved MAAs level was 249.0 mg/L (207.4 mg/L shinorine and 41.6 mg/L of porphyra-334) in YP medium containing 10 g/L glucose and 10 g/L xylose.

Conclusions
Y. lipolytica was successfully engineered to produce MAAs, primarily shinorine. This achievement involved the introduction of MAA biosynthetic genes from cyanobacteria, establishing xylose utilizing pathway, and overexpressing the pentose phosphate pathway genes. These results highlight the potential of Y. lipolytica as a promising yeast chassis strain for MAAs production, notably attributed to its proficient expression of MysE enzyme, which remains non-functional in S. cerevisiae, and versatile utilization of carbon sources like glycerol.
Too Much Information: When Does Additional Testing Benefit Schools?
Authors: Virudachalam Vanitha , Savin Sergei , Steinberg Matthew P. 
Published: November 2023

U.S. K–12 school districts that traditionally utilized ongoing “formative” assessments of student progress increasingly rely on additional “interim” assessments to predict student performance on standardized tests. Moreover, some districts are experimenting with merit-based teacher bonuses tied to standardized test scores. We examine the relationship between interim assessments and teacher bonuses using a two-period principal–agent model. The school district (principal), operating under a limited budget, decides whether to implement interim assessments and how much merit pay to offer, and teachers (agents) choose how much effort to exert in each period. We use two-state (proficient versus not proficient) Markovian dynamics to describe the evolution of student test readiness, in which the transition probability in a given period depends on both teachers’ effort decisions and the starting state. Our results indicate that, despite the popularity of interim assessments, their usefulness is far from guaranteed. In particular, the accuracy promised by these assessments is a double-edged sword: positive midyear results can make it easier to incentivize second period teacher effort, but negative results can have a demotivating effect. Moreover, even when an interim assessment does result in a higher probability of the school ending the year in the proficient state, the resulting higher expected costs of merit-based bonuses for the district may exceed the available budget. Thus, even a free interim assessment might be too expensive for the school district. This paper was accepted by Charles Corbett, operations management. Funding: This research was supported by the Risk Management Center Russell Ackoff Doctoral Student Fellowship and the Fishman-Davidson Center for Service and Operations Management, both at The Wharton School, University of Pennsylvania, Philadelphia, Pennsylvania. Supplemental Material: The online appendix is available at https://doi.org/10.1287/mnsc.2020.01547 .
Atención al nacimiento durante la pandemia COVID-19: restricciones al acompañamiento y cuidados de las madres infectadas
Authors: Ferrándiz Mares Claudia , Llinares Ramal Sandra , Seguí Manzaneque Sandra , Pérez López Alba Isabel , Segura Barrachina Sara , Jareño Roglán Enrique Jesús , Albert i Ros Xavier 
Published: November 2023

Introducción: Los primeros protocolos respecto a la atención al parto y las restricciones de aforo por la pandemia COVID-19 se han relacionado con prácticas no deseables: impedir el acompañamiento en el parto, acelerar el expulsivo, separar madre e hijo o posponer la lactancia materna. El objetivo de este trabajo es valorar la medida en que se han podido dar estas situaciones en nuestro país.
Pacientes y Métodos: 1321 madres que dieron a luz entre marzo de 2019 y mayo de 2021. Estudio descriptivo retrospectivo, basado en encuestas en forma de formularios on-line, sobre infección por SARS-CoV-2, acompañamiento en el parto, forma de dar a luz, alojamiento conjunto y alimentación del bebé.
Resultados: Observamos un significativo incremento del obstáculo al acompañamiento en el parto a partir del 14 de marzo de 2020: 19 %, respecto al 5 % previo (p < 0.001). Entre las madres infectadas por SARS-CoV-2 (17), el 71 % no pudieron estar acompañadas frente al 18 % en las madres no infectadas (p < 0.001); en 3 casos se ha inducido o instrumentado el parto para acelerar el expulsivo y en otros 3 se ha separado a los bebés de sus madres y se les ha alimentado con sucedáneos en la Maternidad.
Conclusiones: Durante la pandemia se ha producido un incremento significativo del impedimento al acompañamiento al parto, significativamente mayor en las madres infectadas por SARS-CoV-2; también hemos detectado entre madres infectadas varios casos de inducción o instrumentación para acelerar el expulsivo, y de separación y empleo de sucedáneos.
Transition metal-based materials and their catalytic influence on MgH2 hydrogen storage: A review
Authors: Gbenebor Oluwashina Philips , Popoola Abimbola Patricia Idowu 
Published: October 2023

The dependence on fossil fuels for energy has culminated in its gradual depletion and this has generated the need to seek alternative source that will be environmentally friendly and sustainable. Hydrogen stands to be promising in this regard as energy carrier which has been proven to be efficient. Magnesium hydride (MgH2) can be used in storing hydrogen because of its availability, light weight and low cost. In this review, monoatomic, alloy, intermetallic and composite forms of Ti, Ni, V, Mo, Fe, Cr, Co, Zr and Nb as additives on MgH2 are discussed. Through ball milling, additive reacts with MgH2 to form compounds including TiH2, Mg2Ni, Mg2NiH4, V2O, VH2, MoSe, Mg2FeH6, NbH and Nb2O5which remain stable after certain de/hydrogenation cycles. Some monoatomic transition metals remain unreacted even after de/hydrogenation cycles. These formed compounds, including stable monoatomic transition metals, impart their catalytic effects by creating diffusion channels for hydrogen via weakening Mg - H bond strength. This reduces hydrogen de/sorption temperatures, activation energies and in turn, hastens hydrogen desorption kinetics of MgH2. Hydrogen storage output of MgH2/transition metal-based materials depend on additive type, ratio of MgH2/additive, ball milling time, ball –to combining materials ratio and de/hydrogenation cycle. There is a need for more investigations to be carried out on nanostructured binary and ternary transition metal-based materials as additives to enhance the hydrogen storage performance of MgH2. In addition, the already established compounds (listed above) formed after ball milling or dehydrogenation can be processed and directly doped into MgH2.
Estimation of water consumption and productivity for wheat using remote sensing and SEBAL model: A case study from central clay plain Ecosystem in Sudan
Authors: Turk Khalid G. Biro , Alsanad Mohammed A. 
Published: November 2023

Abstract
Remote sensing (RS) can efficiently support the quantification of crop water requirements and water productivity (WP) for evaluating the performance of agricultural production systems and provides relevant feedback for management. This research aimed to estimate winter wheat water consumption and WP in the central clay plain of Sudan by integrating remotely sensed images, climate data, and biophysical modelling. The wheat crop was cultivated under a centre-pivot irrigation system during the winter season of 2014/2015. The Landsat-8 satellite data were used to retrieve the required spectral data. The Surface Energy Balance Algorithm for Land (SEBAL) was supported with RS and climate data for estimating the Actual Evapotranspiration (ETa) and the WP for the wheat crop. The SEBAL outputs were validated using the FAO Penman–Monteith method coupled with field measurements and observation. The results showed that the seasonal ETa ranged from 400 to 600 mm. However, the WP was between 1.2 and 1.5 kg/m3 during the wheat cycle. The spatial ETa and WP maps produced by the SEBAL model and Landsat-8 images can improve water use efficiency at field scale environment and estimate the water balance over large agricultural areas.
Choriorétinopathie séreuse centrale associée à l’administration de l’erdafitinib : Rapport de cas
Authors: Blanchard Sierra , Li Suzzane , Weiler Danielle 
Published: November 2023

L'erdafitinib est un inhibiteur de kinase du récepteur du facteur de croissance des fibroblastes (FGFR) qui a été approuvé pour le traitement des patients atteints de carcinome urothélial métastatique ou localement avancé. Il est également à l'étude pour le traitement d'autres types de cancer. Cet agent chimiothérapeutique oral fait l'objet d'une mise en garde concernant les effets indésirables oculaires potentiels, notamment la rétinopathie séreuse centrale et la sécheresse oculaire liées au traitement. Ce rapport de cas décrit un homme caucasien de 88 ans qui a été pris en charge par la clinique ophtalmologique au cours d'un traitement par erdafitinib pour un carcinome urothélial métastatique. Le patient a développé des épisodes asymptomatiques de rétinopathie séreuse centrale bilatérale récurrente et rémittente liée au traitement. Il est essentiel que les fournisseurs de soins oculaires reconnaissent les effets indésirables oculaires potentiels liés à l'erdafitinib, à sa classe de médicaments et aux médicaments qui ciblent des voies similaires. Une communication efficace et une cogestion attentive avec l'oncologie sont essentielles pour fournir des soins de qualité à ce sous-ensemble de patients. Grâce à la coordination des soins, une posologie efficace, mais tolérable peut offrir au patient un meilleur taux de survie et une meilleure qualité de vie.
Наказ №227 «Ні кроку назад» у повсякденній культурі страху сталінізму
Authors: Щелкунов Антон 
Published: November 2023

У спадкоємиці сталінського СРСР – путінської Росії через художні псевдоісторичні твори зобразили штрафбат як сакральний простір у якому кримінальні злочинці перетворюються на справжніх героїв, ціль життя яких вбивати і померти на війні без жодної раціональної мети. Оспівування штрафних підрозділів у сучасній Росії стало однією із основ формування мілітаристської ідеології окупантів, яким все одно за що вмирати і кого вбивати.
Метою дослідження є з’ясування ролі штрафних формувань Червоної армії в роки Другої Світової війни в утвердженні повсякденної культури страху серед радянських людей.
Результати. Розглянуто основні положення наказу № 227, проаналізовано його місце в повсякденній культурі страху. На основі спогадів ветеранів Другої Світової війни показано як страх визначав їх поведінку під час війни та їх ставлення до наказу № 227 вже після її завершення.
Висновки. Сталін і його оточення шукали нові методи посилення повсякденного страху. Результатом цих пошуків стало видання Наказу № 227, «Ні кроку назад» і створення штрафних частин. З того моменту повсякденну культуру страху серед військовослужбовців підпитували не тільки загрози розстрілу чи ув’язнення, а ще й нова форма покарання, яка передбачала залишатися на фронті в значно гірших умовах, адже штрафників не розквартировували у населених пунктах, гірше годували і відправляли на самі важкі ділянки фронту. Тому, Наказ № 227 і штрафні підрозділи слід розглядати не тільки суто з точки зору військової доцільності, а ще з точки зору культурного феномену, який скорегував радянську повсякденність під час Другої Світової війни. Радянська людина повинна була відчувати, що сталінська тоталітарна держава значно сильніша і страшніша за гітлерівську. Цей культурний феномен породив міф про штрафбат, який став частиною ідеології сучасної путінської Росії, в якій штрафбат асоціюється не з жертвами війни і сталінізму, а з кримінальними злочинцями-рецидивістами, які готові вбивати і гинути на війні.
ESTÁGIO SUPERVISIONADO REALIZADO NO CENTRO DE REFERÊNCIA DO IMIGRANTE – BOA VISTA - RORAIMA
Authors: BARBOSA ALVES ANA PAULA , King Chaves Ana Maria , Santos Rodrigues Francilene 
Published: October 2023

Objetivo: Descrever algumas experiências vivenciadas durante o período de estágio supervisionado e as observâncias na dinâmica de funcionamento do Centro de Referência do Imigrante, assim como as dificuldades do povo Warao de acessar os serviços de saúde. Relato de experiência: Este estágio supervisionado foi realizado no Centro de Referência do Imigrante, em que foi possível observar o cotidiano dos migrantes indígenas da etnia Warao no abrigo que estavam alojados. Assim, identificamos várias dificuldades para o encaminhamento às Unidades Básicas de Saúde, do município, quanto para o atendimento destes povos indígenas. Além disso, também participamos das atividades recreativas, como por exemplo o banho nutritivo para combater os piolhos. Conclusões: Observou-se que o povo Warao enfrentam diversos problemas relacionados ao atendimento à saúde, assim como várias outras dificuldades dentro do contexto urbano. Pois, as noções sobre saúde-doença-cuidado para os povos indígenas envolvem complexas relações entre o indivíduo, e a comunidade, sendo diferente das noções da sociedade ocidental.
Palavras-chave: Saúde Coletiva, Direitos dos Povos Indígenas, Migrantes, Acesso aos serviços de Saúde.
ACESSO A MEDICAMENTOS PELA VIA JUDICIAL: REVISÃO SISTEMÁTICA DA LITERATURA ENTRE 2017 E 2021
Authors: Ribas Mirian Cristina , Pedroso Bruno , De Freitas Junior Miguel Arcanjo , Cassins Moreira do Carmo Gonçalo 
Published: October 2023

Introdução: Hodiernamente vem se discutindo em vários segmentos da sociedade a questão da judicialização da saúde que tem crescido de forma exponencial, em especial no que se refere ao fornecimento de medicamentos. Objetivo: Analisar as principais abordagens dos trabalhos científicos nacionais e internacionais publicados entre os anos de 2017 a 2021 sobre o acesso a medicamentos pela via judicial. Método: Revisão sistemática por meio do Methodi Ordinatio, realizada nas bases Web of Science, Scopus e SciElo em que buscou-se artigos publicados entre os anos de 2017 a 2021. Inicialmente, foram encontrados 182 artigos e destes, 31 foram selecionados para análise dos dados. Resultados: Os artigos analisados eram majoritariamente brasileiros e solicitaram medicamentos pela via judicial. Emergiram três categorias: questões que permeiam o acesso a medicamentos pela via judicial, aspectos processuais e medicamentos de alto custo. As solicitações judiciais de medicamentos com custo cada vez mais elevado é uma realidade, e delineia-se um cenário de crescente necessidade de investimentos e a busca de estratégias mais efetivas e eficientes. Conclusão: Ficam indícios no sentido de que se evoluam as políticas públicas de saúde para que haja acesso a medicamentos prescritos, de forma adequada e em tempo hábil à população.
Descritores: Decisões Judiciais; Assistência Farmacêutica; Direito à Saúde.
PERCEPÇÕES DO CONTRIBUTO DA ZONA ECONÔMICA ESPECIAL DE NACALA NA CRIAÇÃO DE EMPREGO PARA AS COMUNIDADES LOCAIS
Authors: Machava Amélia David , Quive Samuel António 
Published: October 2023

Países que criam Zonas Económicas Especiais (ZEEs), em troca, esperam registar aumentos significativos na oferta de emprego para a população local. Entretanto, o objetivo da criação de emprego para os locais pode não ser alcançado, pois as empresas em regime de ZEEs utilizam tecnologias de ponta que exigem uma mão-de-obra qualificada e especializada. Recorrendo à pesquisa mista do tipo sequencial explanatório, o presente estudo visa analisar o contributo de empresas da Zona Económica Especial de Nacala na criação de emprego para as comunidades locais no período de 2009-2020. O estudo revela que a ZEEN não tem registado aumento significado na oferta de emprego para os locais, na medida em que a maior parte dos postos criados não foram ocupados pelos locais, pelo facto das empresas exigirem uma mão-de-obra qualificada e especializada, que é bastante rara entre os membros das comunidades locais. Com base nos resultados, o estudo concluiu que, por um lado, os locais podiam aproveitar as dinâmicas de emprego indireto criadas pela ZEEN, apostando mais na criação de pequenas associações, cooperativas e micro, pequenas e médias empresas, o que seria uma forma de os locais participarem ativa e efetivamente das oportunidades resultantes da implantação da ZEE naquela região. Por outro lado, o Governo devia apostar mais na criação de programas de formação técnico-profissional especializados e certificados para a promoção do capital humano.
Palavras-chave: Zona Económica Especial de Nacala; Emprego, Comunidades Locais
e Moçambique
ANÁLISE DOCUMENTAL DA REDE DE ATENÇÃO PSICOSSOCIAL BRASILEIRA NOS ÚLTIMOS 10 ANOS
Authors: De Oliveira Marcos Vinícius , Harmuch Camila , Paiano Marcelle , Salci Maria Aparecida , Goes Herbert Leopoldo de Freitas , De França Thiago Eduardo , Neto Renato Pereira 
Published: October 2023

Objetivo: analisar os documentos legais promulgados após a implementação da Rede de Atenção Psicossocial, a fim de se constatar os avanços e retrocessos no cuidado em saúde mental brasileiro. Método: pesquisa descritiva de base documental. As fontes documentais utilizadas foram os bancos de dados do Ministério da Saúde e Portal do Governo Federal Brasileiro, a data estabelecida para coleta de dados foi 23 de dezembro de 2011 a 2021. A análise seguiu as três etapas propostas pela análise textual discursiva, sendo: unitarização, categorização e metatextos. Resultados: os documentos promulgados após a instituição da Rede de Atenção Psicossocial indicam um processo de desmonte da mesma. Os hospitais psiquiátricos voltam a ser incentivados financeiramente pelo SUS, bem como as comunidades terapêuticas, em detrimento dos serviços substitutivos. Conclusão: A reformulação e o resgate das políticas públicas em saúde mental é urgente, para que assim os serviços substitutivos de atenção comunitária sejam refortalecidos.
DESCRITORES: Saúde Mental. Serviços de Saúde Mental. Política de Saúde.
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis: A Glimpse into The Future
Authors: Naquib Hannah , Kadiri Sunil Kumar , P Bharath Rathna Kumar , Chikkula Krishna Veni , Srilakshmi Singagari , Poosa Madhuri , Jaiswal Amit , Tiwari Prashant , Kumar Merugumolu Vijay 
Published: October 2023

Background: A class of uncommon IgG4-related systemic diseases known as vasculitis caused by antineutrophil cytosolic antibodies (ANCA) is distinguished by the enlargement of small to large blood vessels. Objective: This review article offers an in-depth analysis of the most recent developments in ANCA-associated vasculitis, covering a range of topics from pathophysiology and diagnostics to treatment and long-term results. Methods: In this review we investigated the existing work on ANCA associated vasculitis by different sources such as Science Direct, Scopus, Pubmed, Web of Science, Google scholar and SciHub. Results: A number of diseases, including eosinophilic granulomatosis with polyangiitis (EGPA), microscopic polyangiitis (MPA), and granulomatosis with polyangiitis (GPA), can have significant morbidity and mortality if they are not appropriately diagnosed and treated. Understanding the pathophysiology, clinical presentation, and curative options for ANCA-associated vasculitis have advanced significantly during the past ten years. The development of specific immunosuppressive medications has been largely responsible for the remarkable evolution in recent years in the treatment of ANCA-associated vasculitis. Conclusion: This review article has provided a detailed examination of the therapy for AAV, alternatives available, including induction and maintenance regimens, as well as the accompanying advantages and disadvantages. Additionally, the growing importance of biologic drugs like rituximab was examined, emphasizing its potential as supplements or replacements for traditional medical treatments.
Театр Шекспира в понимании М. Б. Загорского
Authors: Захаров Николай Владимирович 
Published: October 2023

<p>Статья посвящена рецепции Шекспира в критике советского театрального деятеля М. Б. Загорского. Исследование основано на архивной стенограмме его доклада «Шекспир на советской сцене», прочитанного на заседании Шекспировского кабинета Всероссийского театрального общества 31 октября 1937 г. и других материалах. Наследие Шекспира в русской культуре, театральной критике и литературоведении ХVIII, ХIX и особенно первой половины XX в. нуждается в целостном изучении с учетом его воздействия на театр (режиссерские замыслы, актерское мастерство), теорию перевода, оригинальное творчество отечественных поэтов и писателей, музыку, изобразительные искусства Серебряного века и советского времени.</p><p>М. Б. Загорский уточнял, что в первые годы после революции в Москве Шекспир игрался на семи площадках за один вечер, что очень радовало Луначарского, который тогда говорил, что только у нас Шекспир прорвал замкнутые стены императорских театров и впустил в них новую публику. За сезоны 1917–1927 гг. в Москве прошли все основные пьесы в новых постановках, которые привлекли в театр более 400 тыс. зрителей. По убеждению Загорского, советскому режиссеру, переводчику, актеру, сценографу, театральному музыканту, критику еще предстоит сформировать свой оригинальный эстетический взгляд на творческое наследие драматурга, и это будет борьба за нового советского Шекспира.</p>