bluuebunny/crossref_metadata_2025_split huggingface dataset:
Behavioral Variant Frontotemporal Dementia With the Dominantly Affected Caudate Nucleus in 18F-FP-CIT PET/CT
Authors: Lee Yeongjoo , Byun Seonjeong , Na Sae Jung 
Published: December 2023

Abstract
Frontotemporal dementia is a clinical syndrome that is characterized by a progressive deterioration in behavior, personality, and/or language, with relative preservation of memory, and its phenotype and molecular basis are heterogeneous. We present a case of a 62-year-old female patient who underwent 18F-FDG PET/CT and 18F-FP-CIT PET/CT for differential diagnosis of psychiatric disease and types of dementia. 18F-FDG PET/CT image showed a compatible finding for frontotemporal dementia, and 18F-FP-CIT PET/CT image showed dominantly decreased dopamine transporter activity in the bilateral caudate nucleus.
Pseudodonghicola flavimaris sp. nov. and Sedimentitalea xiamensis sp. nov., two novel species belonging to the family Roseobacteraceae
Authors: Huang Jiamei , Wang Jianning , Li Guangyu , Lai Qiliang , Zhu Xuying , Wang Shanshan , Liu Xiupian , Shao Zongze , Wang Liping 
Published: December 2023

Two Gram-stain-negative, chemoheterotrophic, aerobic bacteria, designated IC7T and JM2-8T, were isolated from seawater of the Yellow Sea of China and rhizosphere soil of mangroves in Xiamen, Fujian, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that these two novel strains belonged to the family

Roseobacteraceae

. Strain IC7T formed a coherent lineage within the genus

Pseudodonghicola

, showing 98.05 % 16S rRNA gene sequence similarity to

Pseudodonghicola xiamenensis

Y-2T. Strain JM2-8T was most closely related to members of the genus

Sedimentitalea

, showing 96.51 and 96.73 % 16S rRNA gene sequence similarities to

Sedimentitalea nanhaiensis

NH52FT and

Sedimentitalea todarodis

KHS03T, respectively. The two novel strains contained Q-10 as the major quinone, and phosphatidylethanolamine, aminophospholipid, phosphatidylglycerol and phosphatidylcholine as the principal polar lipids. The main fatty acid of strain IC7T was C19 : 0 cyclo ω8c, while the fatty acid profile JM2-8T was dominated by summed feature 8 containing C18 : 1
ω7c and/or C18 : 1
ω6c. The average nucleotide identity and digital DNA–DNA hybridization values between these two novel isolates and their closely related species were below the cut-off values of 95–96 and 70 %, respectively. The combined genotypic and phenotypic data show that strain IC7T represents a novel species of the genus

Pseudodonghicola

, for which the name Pseudodonghicola flavimaris sp. nov. is proposed, with the type strain IC7T (=MCCC 1A02763T=KCTC 82844T), and strain JM2-8T represents a novel species of the genus

Sedimentitalea

, for which the name Sedimentitalea xiamensis sp. nov. is proposed, with the type strain JM2-8T (=MCCC 1A17756T=KCTC 82846T).
ggPlantmap: an R package for creation of informative and quantitative ggplot maps derived from plant images
Authors: Jo Leonardo , Kajala Kaisa 
Published: December 2023

AbstractAs plant research generates an ever-growing volume of spatial quantitative data, the need for decentralized and user-friendly visualization tools to explore large and complex datasets tools becomes crucial. Existing resources, such as the Plant eFP (electronic Fluorescent Pictograph) browsers, have played a pivotal role on the communication of gene expression data across many plant species. However, although widely used by the plant research community, the Plant eFP browser lacks open and user-friendly tools for the creation of customized expression maps independently. Plant biologists with less coding experience can often encounter challenges when attempting to explore ways to communicate their own spatial quantitative data. We present ‘ggPlantmap’ an open-source R package designed to address this challenge by providing an easy and user-friendly method for the creation of ggplot representative maps from plant images. ggPlantmap is built in R, one of the most used languages in biology to empower plant scientists to create and customize eFP-like browsers tailored to their experimental data. Here, we provide an overview of the package and tutorials that are accessible even to users with minimal R programming experience. We hope that ggPlantmap can assist the plant science community, fostering innovation and improving our understanding of plant development and function.HighlightggPlantmap, a new addition to the plant data visualization toolbox, allows users to create graphical maps from plant images for the representation of spatial quantitative data in R.
Measurement precision bounds on aberrated single molecule emission patterns
Authors: Fang Li , Huang Fang 
Published: December 2023

ABSTRACTSingle-Molecule Localization Microscopy (SMLM) has revolutionized the study of biological phenomena by providing exquisite nanoscale spatial resolution. However, optical aberrations induced by sample and system imperfections distort the single molecule emission patterns (i.e. PSFs), leading to reduced precision and resolution of SMLM, particularly in three-dimensional (3D) applications. While various methods, both analytical and instrumental, have been employed to mitigate these aberrations, a comprehensive analysis of how different types of commonly encountered aberrations affect single molecule experiments and their image formation remains missing. In this study, we addressed this gap by conducting a quantitative study of the theoretical precision limit for position and wavefront distortion measurements in the presence of aberrations. Leveraging Fisher information and Cramér-Rao lower bound (CRLB), we quantitively analyzed and compared the effects of different aberration types, including index mismatch aberrations, on localization precision in both biplane and astigmatism 3D modalities as well as 2D SMLM imaging. Furthermore, we studied the achievable wavefront estimation precision from aberrated single molecule emission patterns, a pivot step for successful adaptive optics in SMLM through thick specimens. This analysis lays a quantitative foundation for the development and application of SMLM in whole-cells, tissues and with large field of view, providing in-depth insights into the behavior of different aberration types in single molecule imaging and thus generating theoretical guidelines for developing highly efficient aberration correction strategies and enhancing the precision and reliability of 3D SMLM.
Prostate cancer-induced endothelial-to-osteoblast transition generates an immunosuppressive bone tumor microenvironment
Authors: Yu Guoyu , Corn Paul G. , Mak Celia Sze Ling , Liang Xin , Zhang Miao , Troncoso Patricia , Song Jian H. , Lin Song-Chang , Song Xingzhi , Liu Jingjing , Zhang Jianhua , Logothetis Christopher J. , Melancon Marite P. , Panaretakis Theocharis , Wang Guocan , Lin Sue-Hwa 
Published: December 2023

AbstractImmune checkpoint therapy has limited efficacy for patients with bone metastatic castrate-resistant prostate cancer (bmCRPC). In this study, we revealed a novel mechanism that may account for the relative resistance of bmCRPC to immune checkpoint therapy. We found that prostate cancer (PCa)-induced bone via endothelial-to-osteoblast (EC-to-OSB) transition causes an ingress of M2-like macrophages, leading to an immunosuppressive bone tumor microenvironment (bone-TME). Analysis of a bmCRPC RNA-seq dataset revealed shorter overall survival in patients with an M2-high versus M2-low signature. Immunohistochemical (IHC) analysis showed CD206+M2-like macrophages were enriched in bmCRPC specimens compared with primary tumors or lymph node metastasis. In osteogenic PCa xenografts, CD206+macrophages were enriched adjacent to tumor-induced bone. FACS analysis showed an increase in CD206+cells in osteogenic tumors compared to non-osteogenic tumors. Genetic or pharmacological inhibition of the EC-to-OSB transition reduced aberrant bone and M2-like macrophages in osteogenic tumors. RNAseq analysis of tumor-associated macrophages from osteogenic (bone-TAMs) versus non-osteogenic (ctrl-TAMs) tumors showed high expression of an M2-like gene signature, canonical and non-canonical Wnt pathways, and a decrease in an M1-like gene signature. Isolated bone-TAMs suppressed T-cell proliferation while ctrl-TAMs did not. Mechanistically, EC-OSB hybrid cells produced paracrine factors, including Wnts, CXCL14 and LOX, which induced M2 polarization and recruited M2-like TAMs to bone-TME. Our study thus links the unique EC-to-OSB transition as an “upstream” event that drives “downstream” immunosuppression in the bone-TME. These studies suggest that therapeutic strategies that inhibit PCa-induced EC-to-OSB transition may reverse immunosuppression to promote immunotherapeutic outcomes in bmCRPC.SignificanceThe insight that prostate cancer-induced bone generates an immunosuppressive bone tumor microenvironment offers a strategy to improve responses to immunotherapy approaches in patients with bone metastatic castrate-resistant prostate cancer.
The functional evolution of collembolan Ubx on the regulation of abdominal appendage formation
Authors: Liang Yan , Luan Yun-Xia 
Published: December 2023

AbstractFolsomia candidais a tiny soil-living arthropod belonging to the Collembola, which is an outgroup to Insecta. It resembles insects as having a pair of antennae and three pairs of thorax legs, while it also possesses three abdominal appendages: a ventral tube located in the first abdominal segment (A1), retinaculum in A3 and furca in A4. Collembolan Ubx and AbdA specify abdominal appendages, but they are unable to repress appendage marker geneDll. The genetic basis of collembolan appendage formation and the mechanisms by which Ubx and AbdA regulateDlltranscription and appendage development remain unknown. In this study, we analysed the developmental transcriptomes ofF. candidaand identified candidate appendage formation genes, includingUbx(FcUbx). Expression data revealed the dominance ofDlloverUbxduring the embryonic 3.5 and 4.5 days, suggesting Ubx is deficient in suppressingDllat early appendage formation stages. Furthermore, via electrophoretic mobility shift assays and dual luciferase assays, we found that the binding and repression capacity of FcUbx onDrosophila Dllresembles those of the longest isoform ofDrosophilaUbx (DmUbx_Ib), while the regulatory mechanism of the C-terminus of FcUbx onDllrepression is similar to that of crustaceanArtemia franciscanaUbx (AfUbx), demonstrating that the function of collembolan Ubx is intermediate between that of Insecta and Crustacea. In summary, our study provides novel insights into collembolan appendage formation and sheds light on the functional evolution of Ubx. Additionally, we propose a model that collembolan Ubx regulates abdominal segments in a context- specific manner.
The Use of a SOX10 Reporter Towards Ameliorating Oligodendrocyte Lineage Differentiation from Human Induced Pluripotent Stem Cells
Authors: Piscopo Valerio E.C. , Chapleau Alexandra , Blaszczyk Gabriela J. , Sirois Julien , You Zhipeng , Soubannier Vincent , Bernard Geneviève , Antel Jack P. , Durcan Thomas M. 
Published: December 2023

AbstractOligodendrocytes (OLs) are key players in the central nervous system, critical for the formation and maintenance of the myelin sheaths insulating axons, ensuring efficient neuronal communication. In the last decade, the use of human induced pluripotent stem cells (iPSCs) has become essential for recapitulating and understanding the differentiation and role of OLsin vitro. Current methods include overexpression of transcription factors for rapid OL generation, neglecting the complexity of OL lineage development. Alternatively, growth factor-based protocols offer physiological relevance but struggle with efficiency and cell heterogeneity. To address these issues, we created a novel SOX10-P2A-mOrange iPSC reporter line to track and purify oligodendrocyte precursor cells (OPCs). Using this reporter cell line, we analyzed an existing differentiation protocol and shed light on the origin of glial cell heterogeneity. Additionally, we have modified the differentiation protocol, towards enhancing reproducibility, efficiency, and terminal maturity. Our approach not only advances OL biology but holds promise to accelerate research and translational work with iPSC-derived OLs.Main PointsThe differentiation of iPSCs in Oligodendrocyte Precursor Cells (OPCs) and Oligodendrocytes (OLs) is a notoriously difficult technique and often displays variable efficiency and cellular heterogeneity.We engineered a novel reporter line carrying the fluorescent protein mOrange under the control of the OL-specific transcription factor SOX10 to track, purify and characterize OLs.By experimenting with diverse differentiation media, we improved the generation of SOX10-positive cells. Consequently, these cells exhibited increased consistency and effectiveness in evolving into myelinating OLs.
Visual working memories are abstractions of percepts
Authors: Duan Ziyi , Curtis Clayton E. 
Published: December 2023

AbstractPioneering studies demonstrating that the contents of visual working memory (WM) can be decoded from the patterns of multivoxel activity in early visual cortex transformed not only how we study WM, but theories of how memories are stored. For instance, the ability to decode the orientation of memorized gratings is hypothesized to depend on the recruitment of the same neural encoding machinery used for perceiving orientations. However, decoding evidence cannot be used to test the so-calledsensory recruitment hypothesiswithout understanding the underlying nature of what is being decoded. Although unknown during WM, during perception decoding the orientation of gratings does not simply depend on activities of orientation tuned neurons. Rather, it depends on complex interactions between the orientation of the grating, the aperture edges, and the topographic structure of the visual map. Here, our goals are to 1) test how these aperture biases described during perception may affect WM decoding, and 2) leverage carefully manipulated visual stimulus properties of gratings to test how sensory-like are WM codes. For memoranda, we used gratings multiplied by radial and angular modulators to generate orthogonal aperture biases despite having identical orientations. Therefore, if WM representations are simply maintained sensory representations, they would have similar aperture biases. If they are abstractions of sensory features, they would be unbiased and the modulator would have no effect on orientation decoding. Results indicated that fMRI patterns of delay period activity while maintaining the orientation of a grating with one modulator (eg, radial) were interchangeable with patterns while maintaining a grating with the other modulator (eg, angular). We found significant cross-classification in visual and parietal cortex, suggesting that WM representations are insensitive to aperture biases during perception. Then, we visualized memory abstractions of stimuli using a population receptive field model of the visual field maps. Regardless of aperture biases, WM representations of both modulated gratings were recoded into a single oriented line. These results provide strong evidence that visual WM representations are abstractions of percepts, immune to perceptual aperture biases, and compel revisions of WM theory.
N-terminal acetylation separately promotes nuclear localization and host shutoff activity of the influenza A virus ribonuclease PA-X
Authors: Daly Raecliffe E. , Myasnikov Idalia , Gaglia Marta Maria 
Published: December 2023

AbstractTo counteract host antiviral responses, influenza A virus triggers a global reduction of cellular gene expression, a process termed “host shutoff.” A key effector of influenza A virus host shutoff is the viral endoribonuclease PA-X, which degrades host mRNAs. While many of the molecular determinants of PA-X activity remain unknown, a previous study found that N-terminal acetylation of PA-X is required for its host shutoff activity. However, it remains unclear how this co-translational modification promotes PA-X activity. Here, we report that PA-X N-terminal acetylation has two functions that can be separated based on the position of the acetylation, i.e. on the first amino acid, the initiator methionine, or the second amino acid following initiator methionine excision. Modification at either site is sufficient to ensure PA-X localization to the nucleus. However, modification of the second amino acid is not sufficient for host shutoff activity of ectopically expressed PA-X, which specifically requires N-terminal acetylation of the initiator methionine. Interestingly, during infection N-terminal acetylation of PA-X at any position results in host shutoff activity, which is in part due to a functional interaction with the influenza protein NS1. This result reveals an unexpected role for another viral protein in PA-X activity. Our studies uncover a multifaceted role for PA-X N-terminal acetylation in regulation of this important immunomodulatory factor.
LSD1/KDM1A is essential for neural stem cell differentiation in mice
Authors: Falkenberry E.C. , Reeves M. , Scott A. , Myrick D.A , Fallini C. , Bassell G.J. , Katz D.J. 
Published: December 2023

AbstractThe proper regulation of neural stem cell differentiation is required for the proper specification of the central nervous system. Here we investigated the function of the H3K4me1/2 demethylase LSD1/KDM1A during neural stem differentiation in mice. Conditional deletion of LSD1 innestin -positive neural stem cells results in 100% perinatal lethality after birth with severe motor coordination deficits, retarded growth and defects in brain morphology. Despite these severe defects, motor neuron progenitors and the initial motor neuron population are specified normally and motor neurons with normal morphology can be cultured from these micein vitro. However, motor neurons cultured from mice lacking LSD1 in neural stem cells continue to inappropriately maintain critical neural stem cell proteins. Taken together these results suggest that, as in other mouse stem cell populations, LSD1 is required to deactivate the stem cell program to enable normal neural stem cell differentiation. However, unlike in other mouse stem cell populations, the inappropriate maintenance of the stem cell program during neural stem cell differentiation may compromise neuronal function rather than neuronal specification.
Becoming a Multilatina: strategic capabilities as necessary conditions for the internationalization of Latin American firms
Authors: Hermans Michel , Borda Armando , Newburry William , Cordova Chea Carlos Oswaldo , Finchelstein Diego , Gonzalez-Perez Maria Alejandra , Montoya-Bayardo Miguel A. , Velasco Gerardo , Velez-Ocampo Juan 
Published: December 2023

Purpose
This study aims to challenge the generic interpretation of Multilatinas as Latin American firms that have been able to internationalize because of highly competitive strategic capabilities. The authors test whether capabilities that international business researchers commonly associate with internationalization are necessary at different stages of the internationalization process to better understand the extent to which emerging market (EM) firms need to develop them.

Design/methodology/approach
International business research suggests a positive association between strategic capabilities and firm internalization. However, it remains unclear what specific capabilities are necessary and when they are necessary. These questions are particularly important in the context of the internationalization of firms from emerging economies, such as Latin America. The authors apply necessary condition analysis (NCA) on a sample of Latin American firms at different internationalization stages to test what strategic capabilities represent necessary conditions for becoming a Multilatina.

Findings
The findings suggest that only a few strategic capabilities are necessary for Latin American firms to become “Multilatinas”. While entrepreneurial orientation and marketing and sales capabilities represent necessary conditions, EM firms may internationalize even though other capabilities are developed to a lesser extent. The authors reflect on how shifts in local markets and technology drive the emergence of different types of Multilatinas.

Research limitations/implications
Measuring strategic capabilities across multiple EM firms implies a risk that firm-specific aspects are not fully captured. While the authors focused on the comparative competitive strength of capabilities and took great care to minimize measurement error, the authors acknowledge possible bias. Also, while NCA does not require a minimum sample size, findings from our sample of firms from four countries may not generalize to the region or other EMs.

Originality/value
As a relatively new statistical technique, the use of NCA has spread rapidly. To the best of the authors’ knowledge, the linkage between organizational capabilities and firm internationalization has not been tested from a necessary conditions perspective yet. The reflections on the “Multilatina” concept based on the notion of EM firms as configurations of strategic capabilities inform current debates on EM multinational enterprises.
Impact of network embeddedness on innovation performance: the perspective of organisational routine updating
Authors: Zhou Zhigang , Wen Xingxing , Yang Fan 
Published: December 2023

PurposeNetwork embeddedness has been widely considered in enterprise innovation as an effective means of overcoming resource dilemmas. However, while focussing on acquiring external innovation resources, the existing research often ignores the vital role of internal routine updates. Therefore, this study explores the mechanism by which network embeddedness affects innovation performance of enterprises from the perspective of organisational routine updating.Design/methodology/approachThis paper proposes a theoretical model based on social network theory and organisational routines–immune response theory. A total of 328 pieces of research data on high-tech enterprises in China were collected, and the hypotheses were verified using hierarchical regression analysis.FindingsThe results show that the two forms of network embeddedness – structural embeddedness and relational embeddedness, have a positive effect on enterprise innovation performance and a significant positive effect on organisational routine revision and organisational routine creation. Both organisational routine revision and organisational routine creation positively affect enterprise innovation performance and partially mediate the relationship between network embeddedness and enterprise innovation performance.Originality/valueThis conclusion provides a new perspective on the impact of network embeddedness on enterprise innovation performance and expands the related research on organisational routine updating. This study provides a theoretical reference for high-tech enterprises to improve their competitiveness and innovation performance through network embeddedness and organisational routine updating.