bluuebunny/crossref_metadata_2025_split huggingface dataset:
Well Safety Analysis for MPD Operations
Authors: Fernandes A. A. , Sales I. M. , Terra F. S. , Nazareth V. R. , Pena G. C. M. 
Published: October 2023

Abstract
MPD operations have been consistently performed in deep water well construction for +10 years. Some even may advocate it has become commonplace. The consideration of being a fully dominated technology can lead to a false sense of safety. Even though MPD techniques unlock unattainable scenarios, it must not be treated as an ordinary activity.
After a decade of MPD operations, a temporary team was established to analyze the contributing factors of past well safety and integrity occurrences. Unlike other investigations, the strategy adopted by the team was to conduct a broader and systemic analysis of the events.
The way which MPD technique is applied on the Brazilian pre-salt area was analyzed, initially mapping the criticality of the MPD operation and then the interrelationships from design and planning stage to the way construction stage of the wells was conducted. In addition to the analysis of operations, a series of interviews focused on human factors in MPD activities were conducted.
A diagnosis with critical aspects of MPD operations was elaborated and how they socially and systematically contribute to a higher probability in occurrences of incidents during MPD operations.
VprBP/DCAF1 triggers melanomagenic gene silencing through histone H2A phosphorylation
Authors: Shin Yonghwan , Kim Sungmin , Liang Gangning , Ulmer Tobias S , An Woojin 
Published: October 2023

Abstract
Background
Melanoma is the most aggressive form of skin cancer arising from pigment-producing melanocytes and is often associated with dysregulation of epigenetic factors targeting histones. VprBP, also known as DCAF1, is a recently identified kinase and plays an important role in downregulating the transcription of tumor suppressor genes as well as increasing the risk for colon and prostate cancers. However, it remains unknown whether VprBP is also involved in triggering the pathogenesis of other types of cancer.
Results
We demonstrate that VprBP is highly expressed and phosphorylates threonine 120 (T120) on histone H2A to drive transcriptional inactivation of growth regulatory genes in melanoma cells. As is the case for its epigenetic function in colon and prostate cancers, VprBP acts to induce gene silencing program dependently of H2AT120 phosphorylation (H2AT120p). The significance of VprBP-mediated H2AT120p is further underscored by the fact that VprBP knockdown- or VprBP inhibitor-induced lockage of H2AT120p mitigates melanoma tumor growth in xenograft models. Moreover, artificial tethering of VprBP wild type, but not VprBP kinase-dead mutant, to its responsive genes is sufficient for achieving an inactive transcriptional state in VprBP-depleted cells, indicating that VprBP drives gene silencing program in an H2AT120p-dependent manner.
Conclusions
Our results establish VprBP-mediated H2AT120p as a key epigenetic signal for melanomagenesis and suggest the therapeutic potential of targeting VprBP kinase activity for effective melanoma treatment.
Using Machine Learning Methods to Assess the Risk of Alcohol Misuse in Older Adults
Authors: Wickersham Matthew , Bartelo Nicholas , Kulm Scott , Liu Yifan , Zhang Yiye , Elemento Olivier 
Published: October 2023

Abstract
The population of older adults, defined in this study as those 50 years of age or older, continues to increase every year. Substance misuse, particularly alcohol misuse, is often neglected in these individuals. To better identify older adults who might not be properly assessed for alcohol misuse, we have derived a risk assessment tool using patients from the United Kingdom Biobank (UKB), which was validated on patients in the Weill Cornell Medicine (WCM) electronic health record (EHR). The model and tooling created stratifies the risk of alcohol misuse in older adults using 10 features that are commonly found in most EHR systems. We found that the area under the receiver operating curve (AUROC) to correctly predict alcohol misuse in older adults for the UKB and WCM models were 0.84 and 0.78, respectively. We further show that of those who self-identified as having ongoing alcohol misuse in the UKB cohort, only 12.5% of these patients had any alcohol-related F.10 ICD-10 code. Extending this to the WCM cohort, we forecast that 7,838 out of 12,360 older adults with no F.10 ICD-10 code (63.4%) may be missed as having alcohol misuse in the EHR. Overall, this study importantly prioritizes the health of older adults by being able to predict alcohol misuse in an understudied population.
Phosphorylation of the F-BAR protein Hof1 drives septin ring splitting in budding yeast
Authors: Piatti Simonetta 
Published: October 2023

Abstract

A double septin ring at the division site accompanies cytokinesis in yeasts and mammalian cells. In budding yeast, reorganisation of the septin collar at the bud neck into a dynamic double ring, a process we refer to as septin ring splitting, is an essential prerequisite for constriction of the actomyosin ring and, ultimately, for cytokinesis. Septin ring splitting involves the selective disassembly of septin filaments at the bud neck and requires the Mitotic Exit Network (MEN), a Hippo-like kinase cascade essential for cytokinesis. However, the effectors of MEN in this process are unknown. Here we identify the F-BAR protein Hof1 as a critical target of MEN in septin ring splitting. Phospho-mimicking HOF1 mutant alleles overcome the septin splitting defects due to MEN inactivation by decreasing Hof1 binding to septins and facilitating its translocation to the cytokinetic actomyosin ring. Hof1-mediated septin ring splitting depends upon its F-BAR domain, suggesting that it may involve a local membrane reorganisation that leads to septin disassembly. In vitro reconstitution assays show that Hof1 can induce the formation of large networks of intertwined septin bundles, while a phosphomimetic Hof1 protein has impaired septin-bundling activity, likely due to its decreased affinity for septins. Altogether, our data indicate that Hof1 modulates septin architecture in distinct ways depending on its phosphorylation status.
Activation of RMTg projections to the VTA reverse cocaine induced molecular adaptation in the reward system
Authors: Yaka Rami , Khayat Alaa 
Published: October 2023

Abstract
The rostromedial tegmental nucleus (RMTg) plays a crucial role in regulating reward-related behavior by exerting inhibitory control over the ventral tegmental area (VTA). This modulation of dopamine neurons activity within the VTA is essential for maintaining homeostasis in the reward system. Recently we have shown that activation of RMTg projections to the VTA during the acquisition of cocaine-conditioned place preference (CPP) reduces the rewarding properties of cocaine and decreases VTA dopamine neuron activity. By inhibiting dopamine neurons in the VTA, we hypothesized that RMTg projections hold the potential to restore reward system homeostasis disrupted by repeated cocaine use, and attenuate molecular adaptations in the reward system, including alterations in signaling pathways. Our study demonstrates that enhancing the GABAergic inputs from the RMTg to the VTA can mitigate cocaine-induced molecular changes in key regions, namely the VTA, nucleus accumbens (NAc), and prefrontal cortex (PFC). Specifically, we found that cocaine-induced alteration in the phosphorylation state of ERK (pERK) and GluA1 on serine 845 (S845) and serine 831 (S831), that play a major role in plasticity by controlling the activity and trafficking of AMPA receptors, were significantly reversed following optic stimulation of RMTg afferents to the VTA. These findings highlight the therapeutic potential of targeting the RMTg-VTA circuitry for mitigating cocaine reward and addiction. Ultimately, this research may pave the way for novel therapeutic interventions that restore balance in the reward system and alleviate the detrimental effects of cocaine.
Oxidation of DNA and RNA in young patients with newly diagnosed bipolar disorder and relatives
Authors: Coello Klara , Mäkinen Ilari , Kjærstad Hanne , Faurholt-Jepsen Maria , Miskowiak Kamilla Woznica , Poulsen Henrik , Vinberg Maj , Kessing Lars 
Published: October 2023

Abstract
Objective
Excessive oxidative stress-generated nucleoside damage seems to play a key role in bipolar disorder (BD) and may present a trait phenomenon associated with familial risk and is one of the putative mechanisms explaining accelerated atherosclerosis and premature cardiovascular diseases (CVD) in younger patients with BD. However, oxidative stress-generated nucleoside damage has not been studied in young BD patients and their unaffected relatives (UR). Therefore, we compared oxidative stress-generated damage to DNA and RNA in young patients newly diagnosed with BD, UR, and healthy control individuals (HC).
Method
Systemic oxidative stress-generated DNA and RNA damage levels were compared by analyzing urinary levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine and 8-oxo-7,8-dihydroguanosine in participants aged 15 – 25 years, including 133 patients newly diagnosed with BD, 57 UR, and 83 HC.
Results
Compared with HC, damage to DNA was 21.8% higher in BD patients (B = 1.218, 95% CI = 1.111 – 1.335, p=<0.001) and 22.5% higher in UR (B =1.225, 95% CI = 1.090 – 1.377, p=<0.002), while damage to RNA was 14.8% higher in BD patients (B =1.148, 95% CI = 1.082 – 1.219, p=<0.001) and 14.0% higher in UR (B =1.140, 95% CI = 1.055 – 1.230, p=<0.001) in models adjusted for sex and age after correction for multiple comparison. Levels did not differ between patients with BD and UR.
Conclusion
Our findings support higher oxidative stress-generated nucleoside damage being a trait phenomenon in BD associated with familial risk and highlight the importance of early diagnosis and treatment to prevent illness progression and development of premature CVD.
Physical and Mental Health in Adolescence: Novel Insights from a transdiagnostic examination of FitBit data in the ABCD Study
Authors: Damme Katherine , Vargas Teresa , Walther Sebastian , Shankman Stewart , Mittal Vijay 
Published: October 2023

Abstract
Adolescence is among the most vulnerable period for the emergence of serious mental illnesses. Addressing this vulnerability has generated interest in identifying markers of risk for symptoms and opportunities for early intervention. Physical fitness has been linked to psychopathology and may be a useful risk marker and target for early intervention. New wearable technology has made assessing fitness behavior more practical while avoiding recall and self-report bias. Still, questions remain regarding the clinical utility of physical fitness metrics for mental health, both transdiagnostically and along specific symptom dimensions. The current study includes 5007 adolescents (ages 10 to 13) who participated in the Adolescent Brain Cognitive Development (ABCD) study and additional sub-study that collected fitness data from wearable technology and clinical symptom measures. Physical fitness metrics included resting heart rate (RHR- an index of cardiovascular health), time spent sedentary (associated with increased inflammation and cardiovascular disease), and time spent in moderate physical activity (associated with increased neurogenesis, neuroplasticity, and healthy neurodevelopment). Self-report clinical symptoms included measures of internalizing symptoms, externalizing symptoms, and psychosis-like experiences - PLE). Increased RHR- lower cardiovascular fitness- related only to greater internalizing symptoms (t = 3.63). More sedentary behavior related to elevated PLE severity (t = 5.49). More moderate activity related to lower PLE (t=-2.69) and internalizing (t=-6.29) symptom severity. Wearable technology fitness metrics linked physical health to specific mental health dimensions, which emphasizes the utility of detailed digital health data as a marker for risk and the need for precision in targeting physical health behaviors to benefit symptoms of psychopathology.
Extracellular Bioelectrical Lexicon: Detecting Rhythmic Patterns within Dermal Fibroblast Populations
Authors: Gomes Henrique Leonel , Félix Rute , Medeiros Maria , Elamine Youssef , Power Deborah 
Published: October 2023

Abstract
There is growing awareness of the potential role of bioelectrical signals in the fields of cell and tissue physiology. The present study demonstrates that the electrophysiology of populations of non-electrogenic cells in vitro provides reliable information about their status. Non-electrogenic cells synchronize their activity and generate well-defined patterns of bioelectrical signals and the frequency and amplitude of the bioelectrical patterns are strongly dependent on cell activity. Healthy and compact cell monolayers have residual bioelectrical activity. Cells forming or repairing a damaged monolayer generate a diverse range of signal bursts, consisting of quasi-periodic signals in the ultra-low frequency range down to 0.17 mHz (96 minutes). The study contributes a lexicon of bioelectrical signals linked to cell states for short-range non humoral communication and is of relevance for the development of medical devices.
Phosphorylation of Kibra by RSK regulates binding to Cdk4 to control cell cycle progression and organ growth independently of the Hippo-pathway
Authors: Krahn Michael , Wellenberg Lennart , John Jana , Maximowitsch Vanessa , Stiewe Marion , Dickmann Kira , Kalka Marie-Viktoria , Fredrich Ina , Zobel Thomas , Westphal Florian , Wennmann Dirk , Wehr Michael , Pavenstädt Hermann , Kremerskothen Joachim 
Published: October 2023

Abstract
The conserved adapter protein KIBRA (Kidney and Brain) has been described as an upstream regulator of the Hippo signaling cascade, which controls cell proliferation, apoptosis, differentiation and organ growth. Components of this pathway, including KIBRA, are often downregulated or mutated in various types of cancer. KIBRA is phosphorylated at a conserved threonine residue by Ribosomal S6 kinase (RSK), but the function of this phosphorylation in vivo is still unclear. In this study we show that overexpression of Kibra in Drosophila eyes and wings decreases organ growth and that this effect is strongly enhanced upon mutation of the RSK-phosphorylation site in Kibra. Notably, the reduced cell proliferation that leads to impaired organ growth does not depend on the activity of Yorkie as the downstream effector of the Hippo signaling cascade. Instead, Kibra phosphorylation by RSK enables binding to 14-3-3 proteins, which displace Cyclin-dependent kinase 4 (Cdk4) from Kibra, resulting in cell cycle progression. Consequently, overexpression or knockin of a non-phosphorylatable Kibra variant blocks release of Cdk4 from Kibra, retaining cells in G1 phase, which leads to a decreased cell proliferation and thus inhibition of organ and organism growth. Our results elucidate a novel, Hippo pathway-independent function of Kibra in cell cycle regulation and control of organ growth.
Efficacy of 3-D reconstruction assisted ERCP biliary drainage for resectable hilar cholangiocarcinoma
Authors: Xu Lei , Zhang Bin , He Qibin , Mao Liang , Qiu Yudong , Wang Lei , Wang Yi 
Published: October 2023

Abstract
Background and aims:
ERCP-guided biliary drainage (ERCP-BD) is a standard treatment for malignant biliary obstruction. For the drainage of hilar cholangiocarcinoma (HCCA), ERCP-BD has always been controversial due to the high incidence of postoperative cholangitis. In this study, we used a 3-D reconstruction technology (IQQA®-liver) to provide an accurate spatial conformation between tumors and bile ducts, and significantly reduced the incidence of cholangitis.
Methods
A total of 12 patients with HCCA were included in this study during 2017–2018. All patients underwent thin-layer CT scan and MRCP before ERCP-BD to identify the obstruction site and tumor location. The 3-D imaging system was used to accurately locate the tumors and bile ducts. Based on it, we selected the appropriate drainage of hepatic lobes. Intraoperative air cholangiography and nasobiliary drainage were performed, and nasojejunal tubes were placed for external biliary recycle.
Results
All patients underwent successful ERCP procedure and were placed with nasobiliary drainage. One patient also underwent plastic biliary stent implantation. Intraoperative, 4 patients were placed with pancreatic duct stents and 3 patients underwent small endoscopic sphincterotomy (EST). After ERCP, 1 patient presented with mild pancreatitis and cholangitis. No patients received additional percutaneous transhepatic biliary drainage (PTBD) due to insufficient drainage. Except two patients, the remaining patients received radical surgical treatment.
Conclusion
The 3-D reconstruction assisted precision ERCP-BD is a safe and effective method, it can reduce the risk of post-ERCP cholangitis in HCCA patients. Precision ERCP-BD might be justified as a routine procedure for HCCA patients with hyperbilirubinemia.
A Study of the Differential Impact of Macro-Urban Development on Residential Indoor Air Pollution
Authors: Li Chen , feng jinmei , li qinan , yin shiyou , zhang xinyu 
Published: October 2023

Abstract

The data distribution characteristics of indoor air pollutant concentrations in Beijing, Changchun, Shanghai, Jinan, and Zhengzhou were analyzed by quartile partitioning and combined with the composite indoor air quality index (IAQI). Finally, the indoor air quality evaluation equations were established based on the results. The entropy value method is used to construct a coupled and coordinated urban development model and combined with the Tapio elastic decoupling model to macroscopically analyze the impact of urban development factors on indoor air quality. The study results show that the indoor air pollutant concentration data of the five cities are overall high, with different degrees of deviation from the mean, and the pollutants that contribute the most are TVOC and formaldehyde. In descending order, the average indoor air quality index (IAQI) values from the results are Changchun, Shanghai, Beijing, Jinan, and Zhengzhou; based on the results of the study, the recommended pollutant concentration limits to keep the overall IAQI below the mean are formaldehyde (0.063 mg/m³), benzene (0.011 mg/m³), toluene (0.027 mg/m³), xylene (0.03 mg/m³) and TVOC (0.291 mg/m³); Beijing and Shanghai have a high degree of coupled and coordinated urban development and serious indoor air pollution. Zhengzhou and Jinan showed mainly strong negative decoupling and expansion negative decoupling of real estate construction and economic and urbanization level development during the study years, and the economy and real estate construction were disconnected from the urbanization development, and indoor air pollution was lighter in the two cities. In Changchun, there was a "property boom" in 17-19, and in a cold region, indoor ventilation was poor, and indoor air pollution increased significantly.
The yield of SNP microarray analysis for fetal ultrasound cardiac abnormalities
Authors: Ye Fenlei , Xu Xiayuan , Wang Yi , Chen Lifang , Shan Qunda , Jin Fan , Wang Qijing 
Published: October 2023

Abstract
Objective
This study aimed to evaluate the effectiveness of single nucleotide polymorphism microarray (SNP-Array) in the etiological diagnosis of fetal cardiac ultrasound abnormalities.
Methods
A total of 2092 pregnant women who underwent prenatal diagnosis from 2017 to 2022 were included in the study and divided into four groups based on the presence of ultrasound structural abnormalities and the type of abnormality. The results of the SNP-Array test in amniotic fluids in different groups were analyzed.
Results
The non-CHD had the highest incidence of aneuploidy, overall chromosomal abnormalities, and trisomy 18, showing significant statistical differences compared to the other groups(p＜0.001). In terms of CNV segment size distribution frequency, there was no statistical difference between the isolated congenital heart defect group and the non-isolated congenital heart defect group (p＞0.05). The occurrence rates of 22q11.2 and 15q11.2 were also not statistically different between the isolated congenital heart defect group and the non-isolated congenital heart defect group (p＞0.05).
Conclusion
SNP-Array improves the ability to detect of abnormal CNVs in CHD fetuses, providing valuable assistance in diagnosing chromosomal etiology and genetic counseling.
Has the construction of national central cities improved the carbon productivity of urban cluster? Empirical evidence from China
Authors: Li Huafu , Wang Sufeng , Cao Kaixin 
Published: October 2023

Abstract
The national central city is the highest level within China's urban system, bearing the significant responsibility of leading national and regional coordinated development. Based on panel data from 19 city clusters and 207 cities in China spanning the years 2003 to 2019, we treat China's national central city construction policy as a quasi-natural experiment. It employs a difference-in-differences model and a mediation effects model to evaluate the impact mechanism of national central city construction on urban cluster carbon productivity. The result shows that, firstly, the national central city construction significantly enhances urban cluster carbon productivity, a result robustly validated through various sensitivity tests. Secondly, heterogeneity analysis demonstrates that national central city construction exerts a positive influence on the carbon productivity of city clusters in the eastern and central regions. Notably, the effect is more pronounced in the eastern region, whereas it exhibits a distinct inhibitory effect on city clusters in the western region. Overall, the policy effect follows a "U"-shaped trend from west to east. Moreover, a positive correlation emerges between policy effects and city scale, implying that larger cities within city clusters manifest more prominent policy effects stemming from national central city construction. Thirdly, the mechanism of action suggests that national central city construction primarily enhances carbon productivity by augmenting technological innovation capabilities and expediting functional division within urban clusters. Based on the aforementioned findings, it is recommended to actively advance national central city construction, further elevate the technological level of national central cities, and sustain the role of national central cities in driving functional specialization within urban clusters.
Cerebellar Thrombosis Developing After Desmopressin Administration using for the diagnosis of cushing syndrome.
Authors: Houssein Mehdi , Tam Abbas Ali , AYDIN Cevdet , ÇAKIR Bekir 
Published: October 2023

Abstract
Cushing’s syndrome (CS) is a syndrome due to long-term glucocorticoid excess and characterized of signs and symptoms such as moon face, obesity, hypertension, fatigue, amenorrhea, hirsutismus, easy injury, striae. The most common cause of CS is iatrogenic ; when it exclused, endogenous CS occurs as a result of secretion of ACTH and CRH from pituitary and non-pituitary sources and to a lesser extent excessive secretion of glucocorticoids as a result of the pathology of the adrenal gland. Although it is a rare disease, it is an important to be diagnosed, treated and properly followed -up because it causes significant morbidity and mortality when untreated. The diagnosis of CS is based on demonstration of both physical and biochemical features of glucocorticoid excess. For this purpose, primary and secondary diagnostic tests are used. Among the secondary diagnostic tests, desmopressin test is one of the tests used in the differentiation between CS and pseudo-cushing and also in the diagnosis of cushing's disease. Studies concluded that low- desmopressin is safe and well tolerated in patients with no serious adverse effects reported yet. Here we present a case of a 41-year-old woman who developed cerebellar thrombosis shortly after desmopressin administration.