bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep learning framework for automated goblet cell density analysis in in-vivo rabbit conjunctiva
Authors: Jang Seunghyun , Kim Seonghan , Lee Jungbin , Choi Wan Jae , Yoon Chang Ho , Yang Sejung , Kim Ki Hean 
Published: December 2023

AbstractGoblet cells (GCs) in the conjunctiva are specialized epithelial cells secreting mucins for the mucus layer of protective tear film and playing immune tolerance functions for ocular surface health. Because GC loss is observed in various ocular surface diseases, GC examination is important for precision diagnosis. Moxifloxacin-based fluorescence microscopy (MBFM) was recently developed for non-invasive high-contrast GC visualization. MBFM showed promise for GC examination by high-speed large-area imaging and a robust analysis method is needed to provide GC information. In this study, we developed a deep learning framework for GC image analysis, named dual-channel attention U-Net (DCAU-Net). Dual-channel convolution was used both to extract the overall image texture and to acquire the GC morphological characteristics. A global channel attention module was adopted by combining attention algorithms and channel-wise pooling. DCAU-Net showed 93.1% GC segmentation accuracy and 94.3% GC density estimation accuracy. Further application to both normal and ocular surface damage rabbit models revealed the spatial variations of both GC density and size in normal rabbits and the decreases of both GC density and size in damage rabbit models during recovery after acute damage. The GC analysis results were consistent with histology. Together with the non-invasive high-contrast imaging method, DCAU-Net would provide GC information for the diagnosis of ocular surface diseases.
Development and implementation of the frog-in-maze game to study upper limb movement in people with Parkinson’s disease
Authors: Virmani Tuhin , Kemp Aaron S. , Pillai Lakshmi , Glover Aliyah , Spencer Horace , Larson-Prior Linda 
Published: December 2023

AbstractUpper-limb bradykinesia occurs early in Parkinson’s disease (PD) and bradykinesia is required for diagnosis. Our goal was to develop, implement and validate a game “walking” a frog through a maze using bimanual, alternating finger-tapping movements to provide a salient, objective, and remotely monitorable method of tracking disease progression and response to therapy in PD. Twenty-five people with PD and 16 people without PD participated. Responses on 5 different mazes were quantified and compared to spatiotemporal gait parameters and standard disease metrics in these participants. Intertap interval (ITI) on maze 2 & 3, which included turns, was strongly inversely related to stride-length and stride-velocity and directly related to motor UPDRS scores. Levodopa decreased ITI, except in maze 4. PD participants with freezing of gait had longer ITI on all mazes. The responses quantified on maze 2 & 3 were related to disease severity and gait stride-length, were levodopa responsive, and were worse in people with freezing of gait, suggesting that these mazes could be used to quantify motor dysfunction in PD. Programming our frog-in-maze game onto a remotely distributable platform could provide a tool to monitor disease progression and therapeutic response in people with PD, including during clinical trials.
Endophytic microbiota and ectomycorrhizal structure of Alnus glutinosa Gaertn. at saline and nonsaline forest sites
Authors: Thiem Dominika , Goebel Marc , Gołębiewski Marcin , Baum Christel , Koczorski Piotr , Szymańska Sonia , Hrynkiewicz Katarzyna 
Published: December 2023

AbstractThe tolerance of European alder (Alnusglutinosa Gaertn.) to soil salinity can be attributed to symbiosis with microorganisms at the absorptive root level. However, it is uncertain how soil salinity impacts microbial recruitment in the following growing season. We describe the bacterial and fungal communities in the rhizosphere and endosphere of A.glutinosa absorptive roots at three tested sites with different salinity level. We determined the morphological diversity of ectomycorrhizal (ECM) fungi, the endophytic microbiota in the rhizosphere, and the colonization of new absorptive roots in the following growing season. While bacterial diversity in the rhizosphere was higher than that in the absorptive root endosphere, the opposite was true for fungi. Actinomycetota, Frankiales, Acidothermus sp. and Streptomyces sp. were more abundant in the endosphere than in the rhizosphere, while Actinomycetota and Acidothermus sp. dominated at saline sites compared to nonsaline sites. Basidiomycota, Thelephorales, Russulales, Helotiales, Cortinarius spp. and Lactarius spp. dominated the endosphere, while Ascomycota, Hypocreales and Giberella spp. dominated the rhizosphere. The ECM symbioses formed by Thelephorales (Thelephora, Tomentella spp.) constituted the core community with absorptive roots in the spring and further colonized new root tips during the growing season. With an increase in soil salinity, the overall fungal abundance decreased, and Russula spp. and Cortinarius spp. were not present at all. Similarly, salinity also negatively affected the average length of the absorptive root. In conclusion, the endophytic microbiota in the rhizosphere of A.glutinosa was driven by salinity and season, while the ECM morphotype community was determined by the soil fungal community present during the growing season and renewed in the spring.
Blind people can actively manipulate virtual objects with a novel tactile device
Authors: Memeo Mariacarla , Sandini Giulio , Cocchi Elena , Brayda Luca 
Published: December 2023

AbstractFrequently in rehabilitation, visually impaired persons are passive agents of exercises with fixed environmental constraints. In fact, a printed tactile map, i.e. a particular picture with a specific spatial arrangement, can usually not be edited. Interaction with map content, instead, facilitates the learning of spatial skills because it exploits mental imagery, manipulation and strategic planning simultaneously. However, it has rarely been applied to maps, mainly because of technological limitations. This study aims to understand if visually impaired people can autonomously build objects that are completely virtual. Specifically, we investigated if a group of twelve blind persons, with a wide age range, could exploit mental imagery to interact with virtual content and actively manipulate it by means of a haptic device. The device is mouse-shaped and designed to jointly perceive, with one finger only, local tactile height and inclination cues of arbitrary scalar fields. Spatial information can be mentally constructed by integrating local tactile cues, given by the device, with global proprioceptive cues, given by hand and arm motion. The experiment consisted of a bi-manual task, in which one hand explored some basic virtual objects and the other hand acted on a keyboard to change the position of one object in real-time. The goal was to merge basic objects into more complex objects, like a puzzle. The experiment spanned different resolutions of the tactile information. We measured task accuracy, efficiency, usability and execution time. The average accuracy in solving the puzzle was 90.5%. Importantly, accuracy was linearly predicted by efficiency, measured as the number of moves needed to solve the task. Subjective parameters linked to usability and spatial resolutions did not predict accuracy; gender modulated the execution time, with men being faster than women. Overall, we show that building purely virtual tactile objects is possible in absence of vision and that the process is measurable and achievable in partial autonomy. Introducing virtual tactile graphics in rehabilitation protocols could facilitate the stimulation of mental imagery, a basic element for the ability to orient in space. The behavioural variable introduced in the current study can be calculated after each trial and therefore could be used to automatically measure and tailor protocols to specific user needs. In perspective, our experimental setup can inspire remote rehabilitation scenarios for visually impaired people.
Untangling adaptive functioning of PMM2-CDG across age and its impact on parental stress: a cross-sectional study
Authors: Epifani Florencia , Pujol Serra Susana María , Llorens Marta , Balcells Sol , Nolasco Gregorio , Bolasell Mercè , Aguilera-Albesa Sergio , Cancho Candela Ramon , Cuevas Cervera José Luis , García Sánchez Verónica , Garcia Oscar , Miranda-Herrero María Concepción , Moreno-Lozano Pedro J. , Robles Bernabé , Roldán Aparicio Susana , Velázquez Fragua Ramón , Serrano Mercedes 
Published: December 2023

AbstractPhosphomannomutase deficiency (PMM2-CDG) leads to cerebellar atrophy with ataxia, dysmetria, and intellectual deficits. Despite advances in therapy, the cognitive and adaptive profile remains unknown. Our study explores the adaptive profile of 37 PMM2-CDG patients, examining its association with parental stress and medical characteristics. Assessment tools included ICARS for the cerebellar syndrome and NPCRS for global disease severity. Behavioral and adaptive evaluation consisted of the Vineland Adaptive Behavior Scale and the Health of the Nation Outcome Scales. Psychopathological screening involved the Child Behavior Checklist and the Symptom Check-List-90-R. Parental stress was evaluated using Parental Stress Index. Results were correlated with clinical features. No significant age or sex differences were found. ‘Daily living skills’ were notably affected. Patients severely affected exhibited lower adaptive skill values, as did those with lipodystrophy and inverted nipples. Greater severity in motor cerebellar syndrome, behavioral disturbances and the presence of comorbidities such as hyperactivity, autistic features and moderate-to-severe intellectual disability correlated with greater parental stress. Our study found no decline in adaptive abilities. We provide tools to assess adaptive deficits in PMM2-CDG patients, emphasizing the importance of addressing communication, daily living skills, and autonomy, and their impact on parental stress in clinical monitoring and future therapies.
A mirror therapy system using virtual reality and an actuated exoskeleton for the recovery of hand motor impairments: a study of acceptability, usability, and embodiment
Authors: Abbate Gabriele , Giusti Alessandro , Randazzo Luca , Paolillo Antonio 
Published: December 2023

AbstractHand motor impairments are one of the main causes of disabilities worldwide. Rehabilitation procedures like mirror therapy are given crucial importance. In the traditional setup, the patient moves the healthy hand in front of a mirror; the view of the mirrored motion tricks the brain into thinking that the impaired hand is moving as well, stimulating the recovery of the lost hand functionalities. We propose an innovative mirror therapy system that leverages and couples cutting-edge technologies. Virtual reality recreates an immersive and effective mirroring effect; a soft hand exoskeleton accompanies the virtual visual perception by physically inducing the mirrored motion to the real hand. Three working modes of our system have been tested with 21 healthy users. The system is ranked as acceptable by the system usability scale; it does not provoke adverse events or sickness in the users, according to the simulator sickness questionnaire; the three execution modes are also compared w.r.t. the sense of embodiment, evaluated through another customized questionnaire. The achieved results show the potential of our system as a clinical tool and reveal its social and economic impact.
The genus Rachicladosporium: introducing new species from sooty mould communities and excluding cold adapted species
Authors: Piątek Marcin , Stryjak-Bogacka Monika , Czachura Paweł , Owczarek-Kościelniak Magdalena 
Published: December 2023

AbstractThe fungal genus Rachicladosporium (Cladosporiales, Cladosporiaceae), typified by cladosporium-like Rachicladosporium luculiae, includes a morphologically diverse assemblage of species. The species of this genus were reported from different substrates, habitats and environments, including plant leaves and needles, twig, black mould on baobab trees, rocks and insects. In this study, four new Rachicladosporium species (R. europaeum, R. ignacyi, R. kajetanii, R. silesianum) isolated from sooty mould communities covering leaves and needles of trees and shrubs in Poland are described. The new species are delineated based on morphological characteristics and molecular phylogenetic analyses using concatenated ITS, LSU, and rpb2 sequences. All newly described species are nested in the main Rachicladosporium lineage (centred around the type species), which contains species that are able to grow at 25 °C. By contrast, four cold adapted, endolithic species known from Antarctica (R. antarcticum, R. aridum, R. mcmurdoi) and Italian Alps (R. monterosanum) form distant phylogenetic lineage and do not grow at this temperature. Therefore, they are accommodated in the new genus Cryoendolithus, typified by Cryoendolithus mcmurdoi.
Effects of exogenous bile acids (BAs) on growth, lipid profile, digestive enzymes, and immune responses of thinlip mullet, Liza ramada
Authors: Abdel-Tawwab Mohsen , Abdel-Latif Hany M. R. , Basuini Mohammed F. El , El-Nokrashy Asmaa M. , Khaled Asmaa A. , Kord Mohamed , Soliman Ali A. , Zaki Mohamed , Nour Abd-Elaziz , Labib Eman M. H. , Khalil Hala Saber 
Published: December 2023

AbstractAn eight-week trial was designed to explore the dietary effects of commercially purchased exogenous bile acids (BAs) on growth, whole-body composition, lipid profile, intestinal digestive enzymes, liver function enzymes, oxidative stress biomarkers, and serum immunity of thinlip mullet, Liza ramada. Four triplicate groups (10.50 ± 0.05 g) were fed four soybean meal (SBM)-based diets supplied with several BAs levels at 0 (control), 50, 130, or 350 mg/kg feed. Results indicated that the growth was significantly increased in groups fed BAs-based diets, especially at 130 mg/kg feed. The body composition analysis showed that feeding fish on diets supplied with BAs up to 130 mg/kg decreased moisture (%) alongside increased crude protein (%). However, the body composition of fish fed a diet with 350 mg BAs/kg had the lowest moisture (%) and the highest crude protein (%). Moreover, there were significant increases in the intestinal (protease, α-amylase, and lipase) enzyme activities in the groups supplied with BAs up to 130 mg BAs/kg. Liver function enzymes (aspartate aminotransferase and alanine aminotransferase enzyme activities) were significantly decreased in BAs-supplemented groups compared to those fed the BAs-free group. On the other hand, the control group had higher total cholesterol, triglycerides, and low-density lipoprotein alongside the lower high-density lipoprotein than BAs-supplemented groups, especially at 350 mg BAs/kg feed. BAs significantly decreased hepatic malondialdehyde concentrations and increased the activity of hepatic catalase, superoxide dismutase, and total antioxidant capacity compared with those reared on the control diet. Serum lysozyme, respiratory burst, and alternative complement activities were significantly increased in BAs-supplied groups, particularly in the group supplied with 130 mg BAs/kg compared to those fed on the control diet. Accordingly, our findings recommend that including 130 mg BAs/kg in an SBM-based diet enhanced the growth, digestive enzyme activities, and liver functions, alleviated oxidative stress, boosted serum immunity, and lowered lipid metabolites in thinlip mullet. These findings will be beneficial for improving the quality of feed prepared for feeding mullets and an effective alternative strategy to support mullet farming.
Biological dispersion in the time domain using finite element method software
Authors: Guedert Raul , Andrade Daniella L. L. S. , Pintarelli Guilherme B. , Suzuki Daniela O. H. 
Published: December 2023

AbstractBiological tissue exhibits a strong dielectric dispersion from DC to GHz. Implementing biological dispersion in the time domain with commercial finite element method software could help improve engineering analysis of electrical transient phenomena. This article describes the steps required to implement time-domain biological dispersion with commercial finite element method software. The study begins with the presentation of a genetic algorithm to fit the experimental dispersion curve of Solanum tuberosum (potato tuber) to multipoles of first-order Debye dispersion. The results show that it is possible to represent the biological dispersion of S. tuberosum from 40 Hz to 10 MHz in a 4-pole Debye dispersion. Then, a set of auxiliary differential equations is used to transform the multipole Debye dispersion from the frequency domain to the time domain. The equations are implemented in the commercial software COMSOL Multiphysics. A comparison between the frequency and time domain simulations was used to validate the method. An analysis of the electric current with square-wave pulsed voltage was performed. We found that the computer implementation proposed in this work can describe the biological dispersion and predict the electric current.
Hsa-microRNA-1249-3p/Homeobox A13 axis modulates the expression of β-catenin gene in human epithelial cells
Authors: Mazziotta Chiara , Iaquinta Maria Rosa , Tramarin Maria Letizia , Badiale Giada , Cervellera Christian Felice , Tonnini Giulia , Patergnani Simone , Pinton Paolo , Lanza Giovanni , Gafà Roberta , Tognon Mauro , Martini Fernanda , De Mattei Monica , Rotondo John Charles 
Published: December 2023

AbstractIntercellular adhesion is a key function for epithelial cells. The fundamental mechanisms relying on epithelial cell adhesion have been partially uncovered. Hsa-microRNA-1249-3p (hsa-miR-1249-3p) plays a role in the epithelial mesenchymal transition in carcinoma cells, but its physiological function in epithelial cells is unknown. We aimed to investigate the role and molecular mechanisms of hsa-miR-1249-3p on epithelial cell functions. Hsa-miR-1249-3p was overexpressed in human epithelial cells and uterine cervical tissues, compared to cervical carcinoma cells and precancerous tissues, respectively. Hsa-miR-1249-3p was analyzed to verify its regulatory function on Homeobox A13 (HOXA13) target gene and its downstream cell adhesion gene β-catenin. Functional experiments indicated that hsa-miR-1249-3p inhibition prompted the mRNA and protein overexpression of HOXA13 which, in turn, led to the β-catenin protein expression. Moreover, hsa-miR-1249-3p inhibition induced a strong colony forming ability in epithelial cells, suggesting the miR involvement in cell adhesion machinery. These data indicate that hsa-miR-1249-3p regulates the expression of HOXA13 and its downstream cell adhesion gene β-catenin, possible resulting in cell adhesion modification in epithelial cells. This study will allow the set-up of further investigations aimed at exploring the relationship between the hsa-miR-1249-3p/HOXA13 axis and downstream cell adhesion genes.
Higher coactivations of lower limb muscles increase stability during walking on slippery ground in forward dynamics musculoskeletal simulation
Authors: Koo Young-Jun , Hwangbo Jemin , Koo Seungbum 
Published: December 2023

AbstractThe energy efficiency theory of human bipedal locomotion has been widely accepted as a neuro-musculoskeletal control method. However, coactivation of agonist and antagonist muscles in the lower limb has been observed during various limb movements, including walking. The emergence of this coactivation cannot be explained solely by the energy efficiency theory and remains a subject of debate. To shed light on this, we investigated the role of muscle coactivations in walking stability using a forward dynamics musculoskeletal simulation combined with neural-network-based gait controllers. Our study revealed that a gait controller with minimal muscle activations had a high probability of falls under challenging gait conditions such as slippery ground and uneven terrain. Lower limb muscle coactivations emerged in the process of gait controller training on slippery ground. Controllers with physiological coactivation levels demonstrated a significantly reduced probability of falls. Our results suggest that achieving stable walking requires muscle coactivations beyond the minimal level of muscle energy. This study implies that coactivations likely emerge to maintain gait stability under challenging conditions, and both coactivation and energy optimization of lower limb muscles should be considered when exploring the foundational control mechanisms of human walking.
Experimental study on Compton camera for boron neutron capture therapy applications
Authors: Sakai M. , Tamaki S. , Murata I. , Parajuli R. K. , Matsumura A. , Kubo N. , Tashiro M. 
Published: December 2023

AbstractBoron neutron capture therapy (BNCT) is a high-dose-intensive radiation therapy that has gained popularity due to advancements in accelerator neutron sources. To determine the dose for BNCT, it is necessary to know the difficult-to-determine boron concentration and neutron fluence. To estimate this dose, we propose a method of measuring the prompt γ-rays (PGs) from the boron neutron capture reaction (BNCR) using a Compton camera. We performed a fundamental experiment to verify basic imaging performance and the ability to discern the PGs from 511 keV annihilation γ-rays. A Si/CdTe Compton camera was used to image the BNCR and showed an energy peak of 478 keV PGs, separate from the annihilation γ-ray peak. The Compton camera could visualize the boron target with low neutron intensity and high boron concentration. This study experimentally confirms the ability of Si/CdTe Compton cameras to image BNCRs.
SET8 is a novel negative regulator of TGF-β signaling in a methylation-independent manner
Authors: Nagasaka Mai , Inoue Yasumichi , Nagao Yuji , Miyajima Chiharu , Morishita Daisuke , Aoki Hiromasa , Aoyama Mineyoshi , Imamura Takeshi , Hayashi Hidetoshi 
Published: December 2023

AbstractTransforming growth factor β (TGF-β) is a multifunctional cytokine that induces a diverse set of cellular processes principally through Smad-dependent transcription. Transcriptional responses induced by Smads are tightly regulated by Smad cofactors and histone modifications; however, the underlying mechanisms have not yet been elucidated in detail. We herein report lysine methyltransferase SET8 as a negative regulator of TGF-β signaling. SET8 physically associates with Smad2/3 and negatively affects transcriptional activation by TGF-β in a catalytic activity-independent manner. The depletion of SET8 results in an increase in TGF-β-induced plasminogen activator inhibitor-1 (PAI-1) and p21 expression and enhances the antiproliferative effects of TGF-β. Mechanistically, SET8 occupies the PAI-1 and p21 promoters, and a treatment with TGF-β triggers the replacement of the suppressive binding of SET8 with p300 on these promoters, possibly to promote gene transcription. Collectively, the present results reveal a novel role for SET8 in the negative regulation of TGF-β signaling.