bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bortezomib improves progression-free survival in multiple myeloma patients overexpressing preferentially expressed antigen of melanoma
Authors: Qin Yazhen , Lu Jin , Bao Li , Zhu Honghu , Li Jinlan , Li Lingdi , Lai Yueyun , Shi Hongxia , Wang Yazhe , Liu Yanrong , Jiang Bin , Huang Xiaojun 
Published: January 2024

Background
Significant efforts have been made to identify factors that differentiate patients treated with novel therapies, such as bortezomib in multiple myeloma (MM). The exact expression pattern and prognostic value of the cancer/testis antigen preferentially expressed antigen of melanoma (PRAME) in MM are unknown and were explored in this study.

Methods
The transcript level of PRAME was detected in bone marrow specimens from 100 newly diagnosed MM patients using real-time quantitative polymerase chain reaction, and the prognostic value of PRAME was determined through retrospective survival analysis. PRAME expression higher than the upper limit of normal bone marrow was defined as PRAME overexpression or PRAME (+).

Results
Sixty-two patients (62.0%) overexpressed PRAME. PRAME overexpression showed no prognostic significance to either overall survival (n=100) or progression-free survival (PFS, n=96, all P >0.05) of patients. The patients were also categorized according to regimens with or without bortezomib. PRAME overexpression tended to be associated with a lower two-year PFS rate in patients treated with non-bortezomib-containing regimens (53.5% vs. 76.9%, P=0.071). By contrast, it was not associated with the two-year PFS rate in patients with bortezomib-containing regimens (77.5% vs. 63.9%, P >0.05). When the patients were categorized into PRAME (+) and PRAME (-) groups, treatment with bortezomib-containing regimens predicted a higher two-year PFS rate in PRAME (+) patients (77.5% vs. 53.5%, P=0.027) but showed no significant effect on two-year PFS rate in PRAME (-) patients (63.9% vs. 76.9%, P >0.05).

Conclusion
PRAME overexpression might be an adverse prognostic factor of PFS in MM patients treated with non-bortezomib-containing regimens. Bortezomib improves PFS in patients overexpressing PRAME.
Validation of glaucoma-like features in the rat episcleral vein cauterization model
Authors: Bai Yujing , Zhu Yingting , Chen Qin , Xu Jing , Sarunic Marinko V. , Saragovi Uri H. , Zhuo Yehong 
Published: January 2024

Background
Glaucoma, an irreversible optic nerve neuropathy, always results in blindness. This study aimed to evaluate glaucoma-like features in the rat episcleral vein cauterization (EVC) model by multiple in vivo and in vitro evidences.

Methods
Wistar rat was used in this study. The elevated intraocular pressure (IOP) was induced by cauterization of three episcleral veins. IOP was monitored with Tono-Pen XL tonometer. Time-dependent changes to the neuronal retinal layers were quantified by Fourier domain-optical coherence tomography. The function of retina was evaluated by electroretinogram (ERG). Survival of retinal ganglion cells (RGCs) was quantified by retrograde labeling. Histology study was performed with retinal sections stained with hematoxylin-eosin, glial fibrillary acidic protein, and neuronal nuclear antigen. Retina and aqueous humor protein were extracted and cytotoxic protein tumor necrosis factor alpha (TNF-α) and alpha-2 macroglobulin (α2m) were measured with Western blotting.

Results
EVC is a relatively facile intervention, with low failure rates (<5%). After surgical intervention, chronic mild IOP elevation (about 1.6-fold over normal, P <0.05) was induced for at least 6 weeks without requiring a second intervention. High IOP causes chronic and progressive loss of RGCs (averaging about 4% per week), progressive thinning of neuronal retinal layers (3-5 μm per week), and reduction of a- and b-wave in ERG. EVC method can also induce glial cell activation and alterations of inflammation proteins, such as TNF-α and α2m.

Conclusion
EVC method can establish a robust, reliable, economic and highly reproducible glaucomatous animal model.
Evaluation of CT findings for the differentiation of benign from malignant primary retroperitoneal tumors
Authors: Zhu Zheng , Zhao Xinming , Zhao Yanfeng , Yang Lei , Zhao Jing , Dai Jingrui , Zhou Chunwu 
Published: January 2024

BackgroundBenign and malignant primary retroperitoneal tumors (RT) have different pathological manifestations, and overlapping imaging characteristics. This study aimed to evaluate the value of computed tomography (CT) for differentiating benign from malignant RT.MethodsOne hundred and ninety-four patients with clinical and radiographic data were evaluated retrospectively following surgical resection of primary RT. There were 38.1% (n=74) benign lesions and 61.9% (n=120) malignant lesions. Categorical variables were tested with a chi-square test or Fisher's exact test for the diagnostic indexes and sensitivity and specificity of CT characteristics.ResultsIn univariate analysis, the differences in ill-defined margins, irregular surfaces, long diameter >6.75 cm, short diameter >6.25 cm, and solid or mixed texture had statistical significance; the sensitivity and specificity were 44.2% and 91.9%, 70.0% and 62.2%, 68.8% and 60.7%, 59.7% and 74.2%, 87.5% and 35.1%, respectively. In multivariate analysis, a combination of all the above indexes was the best model for differentiating malignant tumors, resulting in the most accurate diagnosis of malignancies with a sensitivity of 77.2% and a specificity of 81.1% (P<0.0001) when the score was 4. The differences in other findings including CT attenuation, number, and calcification had no statistical significance. The unique characteristics included the spotted enhancement (mottled high density) in schwannoma (Ρ<0.0001), adipose tissue in liposarcoma (P<0.0001) and paravertebral location in neurogenic tumors (P<0.0001).ConclusionsMore accurate differential diagnosis of primary RT can be made through comprehensive analysis of the combined diagnostic indexes of CT. Some specific characteristics of CT can assist in preoperative planning.
New techniques to improve classical corneal collagen cross-linking treatment
Authors: Na Li , Xiujun Peng , Zhengjun Fan , Yu Xia , Tengfei Wu 
Published: January 2024

Objective
The aim of this review is to comprehensively and unbiasedly summarize the improvements in the techniques for classical corneal collagen cross-linking (CXL) by covering the reasons for this improvement, measure, and effect to approach the future direction of the CXL.

Data sources
All articles used in this review were mainly retrieved from the PubMed database.

Study selection
Original articles and reviews were selected if they were related to the improvement in the technique of classical CXL. Data were mainly extracted from 94 articles, which are listed in the reference section of this review.

Results
This innovative research involves every step such as instrument preparation, epithelial management, riboflavin instillation, and UVA irradiation. These clinical and experimental results seem promising.

Conclusions
CXL treatment is the only recent promising method for preventing the progress of keratoconus. The limitations and potential complications that accompany classical CXL such as corneal thickness limitations, ultraviolet-A (UVA) light injury, and the impact of de-epithelialization encourage people to research new improvements in techniques.
While this research needs to be further investigated, we hope our review can help related researchers and patients.
Photoprotective effect of the N-terminal 5-mer peptide analog P165 of amyloid precursor protein in human dermal fibroblasts
Authors: Wang Ying , Chen Hui , Lin Yuying , Wang Wen , Wang Rong , Lian Shi , Zhu Wei 
Published: January 2024

Background
We showed in our previous study that the N-terminal 17-mer peptide of amyloid precursor protein (APP17-mer peptide), an active peptide segment with trophic and antioxidative effects, protects skin fibroblasts against ultraviolet (UV) damage and downregulates matrix metalloproteinase 1 (MMP-1) expression. The aim of the current study was to explore the protective effects of P165, the N-terminal 5-mer peptide analog of amyloid precursor protein that is resistant to enzymolysis, on UVA-induced damage in human dermal fibroblasts (HDFs).

Methods
HDFs were cultured in Dulbecco’s modified Eagle’s medium without and with P165 (concentrations were 1, 10, and 100 μmol/L). Then, 15 J/cm2 UVA irradiation was used to obtain the UV-irradiated model. Cell proliferation was analyzed using MTT kit. The collagen type I and MMP-1 contents in cell lysate were determined by enzyme-linked immunosorbent assay (ELISA). Fluorometric assays were performed to detect the formation of intracellular reactive oxygen species (ROS) in the cells.

Results
P165 significantly protected the HDFs against UVA-induced cytotoxicity. Compared with the UVA-irradiated control, 1, 10, and 100 μmol/L P165 elevated cell proliferation by 14.98% (P<0.05), 17.52% (P<0.01) and 28.34% (P<0.001), respectively. Simultaneously, 10 and 100 μmol/L P165 increased collagen type I content (both P<0.05). Moreover, P165 treatment (all concentrations) also markedly suppressed the UVA-induced MMP-1 expression (all P<0.001). P165 at 1, 10, and 100 μmol/L also reduced UVA-induced ROS generation by 11.27%, 13.69% (both P<0.05), and 25.48% (P<0.001), respectively.

Conclusions
P165 could protect the HDFs against UVA-induced photodamage, including cytotoxicity, and MMP-1 generation. Furthermore, it also increased the collagen type I content in the cells. The inhibitory effect on intracellular ROS generation might be involved in these photoprotective effects. Thus, P165 may be a useful candidate in the prevention and treatment of skin photoaging.
Relationship of Helicobacter pylori eradication with gastric cancer and gastric mucosal histological changes: a 10-year follow-up study
Authors: Liya Zhou , Sanren Lin , Shigang Ding , Xuebiao Huang , Zhu Jin , Rongli Cui , Lingmei Meng , Yuan Li , Li Zhang , Changji Guo , Yan Xue , Xiu'e Yan , Jing Zhang 
Published: January 2024

Background

Helicobacter pylori (Hp) is a common and potentially curable cause of gastric mucosa lesion. This study investigated the relationship of Hp infection with histological changes in gastric mucosa and gastric cancer in Hp-positive patients compared with Hp-eradication patients followed up for ten years.

Methods
From an initial group of 1 006 adults, 552 Hp-positive subjects were randomly assigned to a treatment group (T; n =276) or a placebo group (P; n =276). In the randomized, double-blind, placebo-controlled, parallel trial, T group subjects received oral doses of omeprazole, amoxicillin and clarithromycin for 1 week; those in the P group received a placebo. One month after treatment ended, a 13C urea breath test was performed, and Hp was undetectable in 88.89% of the T group. All subjects were followed at 1, 5, 8, and 10 years after treatment, with endoscopy and biopsies for histological examination.

Results
Gastric mucosa inflammation was significantly milder in the T group than that in the P group one year after Hp eradication and this persisted for 10 years. Glandular atrophy and intestinal metaplasia (IM) had deteriorated in both groups during ten years. However, the increased score of glandular atrophy at both the gastric antrum and corpus, and IM only at the gastric antrum, in the P group was more obvious than that in the T group. During the 10 years, 9 patients were diagnosed with gastric cancer (2 in the T group; 7 in the P group; P=0.176). When mucosal atrophy was absent at the gastric antrum and corpus when entering the study, the incidence of gastric cancer in the P group (n=6) was much higher than that in the T group (n =0, P=0.013).

Conclusions
Hp eradication may significantly diminish and help halt progression of gastric mucosal inflammation and delay the development of IM and atrophy gastritis. Hp eradication is helpful for reducing the risk for gastric cancer, especially in the early stage of Hp infection.
Twelve years' experience and clinical results of using the radial artery for coronary revascularization
Authors: Cheng Zhaoyun , Zhao Ziniu , Quan Xiaoqiang , Wang Sheng , Zhao Jian , Sun Junjie , Hu Junlong , Gao Chuanyu , Song Yongping 
Published: January 2024

Background
The radial artery (RA) is becoming a popular conduit for coronary artery bypass grafting (CABG), yet data reporting the long-term results are rare. We reported our clinical, angiographic and intravascular ultrasound findings on 93 patients who had the RA used as part of the conduit for the CABG procedures during a 12-year period from June 2001 to June 2013.

Methods
A total of 118 radial artery conduits were harvested in 87 males and 6 females, age from 28 to 66 (mean 49.9) years. An “intra-operative Allen's test” was developed to safeguard blood supply to the arm and hand. A “double-clip & scissors-cut” technique was carried out to minimize the thermal injury to the radial artery from the diathermy. The left radial artery was used in 67 patients, the right in one, and bilateral radial arteries in 25 patients. One hundred and twenty-two out of 272 distal anastomoses (44.9%) were constructed with radial arteries, with an average of 2.9 grafts per patient (range 2-6).

Results
Follow-up angiography and intravascular ultrasound study at 3-139 postoperative months (mean 59 months) revealed a 93.1% RA patency. String sign occurred in one patient in whom the RA was directed to a big right coronary artery with a stenosis of around 50%. The patency for the internal mammary artery was 96.4%.

Conclusions
The RA is an excellent conduit that broadens the options for total arterial CABG surgery. Good graft patency could be achieved through careful harvesting techniques and choice of proper target coronary vessels.
A prospective comparative study on neovascular glaucoma and non-neovascular refractory glaucoma following Ahmed glaucoma valve implantation
Authors: Zheng Li , Minwen Zhou , Wei Wang , Wenbin Huang , Shida Chen , Xingyi Li , Xinbo Gao , Xiulan Zhang 
Published: January 2024

BackgroundNeovascular glaucoma is a refractory disease, and difficult to manage. The aim of this study was to evaluate the clinical outcomes of Ahmed glaucoma valve implantation (AGVI) in neovascular glaucoma (NVG) and non-NVG patients.MethodsThis prospective, non-randomized study included 55 eyes of 55 patients with refractory glaucoma; 27 had NVG (NVG group) and 28 had non-NVG (non-NVG group). All of the patients underwent AGVI. The NVG group was adjunctively injected with intravitreal ranibizumab/bevacizumab (IVR/IVB) before AGVI. Intraocular pressure (IOP) was the primary outcome measure in this study. Surgical success rate, number of antiglaucoma medications used, best corrected visual acuity (BCVA), and postoperative complications were analyzed between the groups.ResultsAll of the patients completed the study (follow-up of 12 months). Kaplan-Meier survival curve analysis indicated that the qualified success rates in the NVG and non-NVG groups at 12 months were 70.5% and 92.9%, respectively; this difference was significant (P=0.036). The complete success rates in the NVG and non-NVG groups at 12 months were 66.7% and 89.3%, respectively (P=0.049). Compared with preoperative examinations, the postoperative mean IOP and use of medications were significantly lower at all follow-up time points in both groups (allP<0.05). There were significant differences in BCVA between the two groups at the 12-month follow-up (χ2=9.86,P=0.020). Cox proportional hazards regression showed NVG as a risk factor for surgical failure (RR=15.08,P=0.033). Postoperative complications were similar between the two groups.ConclusionsAGVI is a safe and effective procedure in refractory glaucoma, but the success rate of surgery was related to the type of refractory glaucoma. The complete and qualified success rates of NVG patient adjunctive anti-vascular endothelial growth factor treatment are still lower than those of non-NVG patients.
Transradial versus transfemoral percutaneous coronary intervention in elderly patients: a systematic overview and meta-analysis
Authors: He Peiyuan , Yang Yuejin , Hu Fenghuan 
Published: January 2024

Background
Transradial approach (TRA) percutaneous coronary intervention (PCI) has been wildly applied among unselected patients. However, only very few small studies have compared the outcomes between TRA and transfemoral approach (TFA) in elderly patients. We aimed to evaluate the efficacy and safety between TRA and TFA in elderly patients by a pooled analysis.

Methods
Studies that met the inclusion and exclusion criteria were included. Statistical analysis was performed using the Review Manager 5.0.0 developed and maintained by the Cochrane Collaboration and a random-effects model was used to better account for the differences among the sub-studies. The primary endpoint was defined as short-term mortality, and other endpoints included major adverse cardiovascular events, major bleeding events, procedure success, vascular complications and hospital stay.

Results
Two thousand one hundred and eighty-eight patients from 11 studies were finally included. A non-significant trend toward a lower rate of short-term death was found in favor of TRA (odds ratio (OR): 0.56, 95% confidence interval (CI): 0.27–1.16). The incidence rates of vascular complications (OR 0.25, 95% CI: 0.14–0.46) and major bleeding events (OR: 0.31, 95% CI: 0.18–0.55) were greatly reduced by TRA compared with TFA. No significant difference was detected in the occurrence rate of major adverse cardiovascular events (OR: 0.77, 95% CI: 0.45–1.30), but the rate of procedure success was significantly improved by TRA (OR: 1.86, 95% CI: 1.18–2.94). In addition, the total hospital stay was also significantly reduced by TRA.

Conclusions
TRA showed greater efficacy and safety compared with TFA in elderly patients. It should be recommend as routine practice for elderly patients undergoing PCI in TRA capable hospitals.
Characterization and influencing factors of visit-to-visit blood pressure variability of the population in a northern Chinese industrial city
Authors: Cao Huijun , Wu Shouling , Li Shuqiang , Zhao Haiyan , Ruan Chunyu , Wu Yuntao , Xing Aijun , Li Kuibao , Chen Jin , Yang Xinchun , Cai Jun 
Published: January 2024

Background
Blood pressure variability (BPV) is a reliable prognostic factor for cardiovascular events. Currently there is a worldwide lack of large sample size studies in visit-to-visit BPV. Based on the Kailuan Study, we analyzed the visit-to-visit BPV of patients to investigate the range and influencing factors of BPV.

Methods
In 11 hospitals in the Kailuan Company, 4 441 patients received routine health checkups. Physical examination measured blood pressure (BP), body height, body weight, and waist circumference, and body mass index was calculated. Blood samples were analyzed for plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG), and high-sensitivity c-reactive protein (hs-CRP).

Results
The effect of gender on systolic BPV was investigated. The average systolic BPV was 10.35 mmHg (1 mmHg= 0.133 kPa) overall, 10.54 mmHg in males and 10.06 mmHg in females. Multivariate Logistic regression analysis revealed that the age (RR=1.022), systolic BP (SBP, RR=1.007), LDL-C (RR=1.098), and history of hypertension (RR=1.273) were significant risk factors for higher systolic BPV. We found that aging (RR=1.022), increased SBP (RR=1.007), and a history of hypertension (RR=1.394) were determinants of systolic BPV in males. The risk factors for systolic BPV of females were aging (RR=1.017), increased SBP (RR=1.009), increased LDL (RR=1.136), and increased TG (RR= 1.157).

Conclusion
Our findings indicated that the systolic BPV is closely associated with age, SBP and history of hypertension.
Cancer immunoinformatics: a new assistant tool for malignant disease research
Authors: Wang Weijia , Zhang Rupeng , Liang Han , Zhang Hui , Li Fangxuan , Yu Jinpu , Li Hui , Ren Xiubao 
Published: January 2024

Objective
To introduce the recent developments in cancer immunoinformatics with an emphasis on the latest trends and future direction.

Data sources
All related articles in this review were searched from PubMed published in English from 1992 to 2013. The search terms were cancer, immunoinformatics, immunological databases, and computational vaccinology.

Study selection
Original articles and reviews those were related to application of cancer immunoinformatics about tumor basic and clinical research were selected.

Results
Cancer immunoinformatics has been widely researched and applied in a series of fields of cancer research, including computational tools for cancer, cancer immunological databases, computational vaccinology, and cancer diagnostic workflows. Furthermore, the improvement of its theory and technology brings an enlightening insight into understanding and researching cancer and helps expound more deep and complete mechanisms of tumorigenesis and progression.

Conclusion
Cancer immunoinformatics provides promising methods and novel strategies for the discovery and development of tumor basic and clinical research.
Diagnosis of brain death: confirmatory tests after clinical test
Authors: Su Yingying , Yang Qinglin , Liu Gang , Zhang Yan , Ye Hong , Gao Daiquan , Zhang Yunzhou , Chen Weibi 
Published: January 2024

Background
The brain death confirmation tests occupy a different position in each country's diagnostic criteria (or guideline); the choices of tests are also different. China brain death criteria include clinical judgment and confirmation tests. This study aimed to confirm the preferred confirmatory test and complementary confirmatory tests.

Methods
We did a clinical brain death determination on deep coma patients, and then divided them into brain death group and non-brain death group. According to the Chinese standards for determining brain death, both the groups accepted confirmatory tests including electroencephalograph (EEG), somatosensory evoked potentials (SEP), and transcranial Doppler (TCD). The sensitivity, specificity, false positive rate, and false negative rate were calculated to evaluate the accuracy of the confirmatory tests.

Results
Among the 131 cases of patients, 103 patients met the clinical criteria of brain death. Respiratory arrest provocation test was performed on 44 cases and 32 cases (73%) successfully completed and confirmed that they have no spontaneous breathing. Of the three confirmation tests, EEG had the highest completion rate (98%) and good sensitivity (83%) and specificity (97%); TCD had followed completion rate (54%) and not good sensitivity (73%) and specificity (75%); SEP had the lowest completion rate (49%), good sensitivity (100%), and not good specificity (78%). After the combination of SEP or TCD with EEG, the specificity can increase to 100%.

Conclusions
The completion rate of respiratory arrest provocation test remains a problem in the clinical diagnosis of brain death. If the test cannot be completed, whether to increase a confirmatory test is debatable. SEP had an ideal sensitivity, and the specificity will reach 100% after combining with TCD or EEG. When a confirmed test was uncertain, we suggest increasing another confirmatory test.