bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhinovirus infects primary human airway fibroblasts and induces a neutrophil chemokine and a permeability factor
Authors: Ghildyal Reena , Dagher Hayat , Donninger Howard , de Silva Dinesha , Li Xun , Freezer Nicholas J. , Wilson John W. , Bardin Philip G. 
Published: February 2005

AbstractThe events linking rhinovirus (RV) infection to airway symptoms are poorly understood. The virus initially infects airway epithelium followed by a vigorous inflammatory response that may entail spread of RV from epithelium to other cells in the airway wall. However, RV has fastidious growth characteristics and to date reproductive infection of primary cells other than human airway epithelium has not been confirmed. Airway fibroblasts are adjacent to and in contact with epithelial cells, play a key role in innate immune responses, and may participate in the evolution of inflammation. To investigate fibroblast actions, we first determined whether RV could infect and replicate in primary culture human lung fibroblasts. RV serotype 16 (RV16) was used to infect fibroblasts grown from lung tissue, and virus infection with replication was demonstrated by a combination of techniques. RT‐PCR was used to show an increase in RV transcription; confocal microscopy demonstrated colocalization of the replicative form of RV genome (double‐stranded RNA) and RV16 proteins; infectious virus was also recovered from the culture supernatant of infected fibroblasts. Functional consequences of RV infection were next examined. RV infection of fibroblasts was followed by an increase in epithelial neutrophil‐activating peptide‐78 (ENA‐78) mRNA and protein. The permeability factor vascular endothelial growth factor (VEGF) was also induced over a similar time course. These data suggest that interactions between RV and human fibroblasts are feasible, may coordinate neutrophil chemoattraction with enhanced vascular permeability and that fibroblasts may contribute to inflammatory responses following RV infections. J. Med. Virol. 75:608–615, 2005. © 2005 Wiley‐Liss, Inc.
Reexamining and extending the dual mediation hypothesis in an on‐line advertising context
Authors: Karson Eric J. , Fisher Robert J. 
Published: February 2005

AbstractThe relationship between attitude toward the advertisement (Aad) and intentions to buy (Ib) is a controversial one. The present research examines the potential for a direct relationship between Aad and Ib within an on‐line advertising context, substituting Asite (attitude toward the Web site) for Aad. The article replicates previous findings with respect to the four competing Aad models they tested. The article then predicts and finds a significant Asite → Ib path based on an opportunity and motivation perspective. In terms of opportunity, Web sites contain nonproduct information that is independent of traditional measures of brand attitude (Ab), such as security, ease of use, transactional capabilities, which are likely to affect Ib. Further, on‐line consumers are motivated to process this information when they visit a Web site because they cannot directly examine the products they are considering. Evidence for the opportunity and motivation perspective is provided by the decomposition of Asite into its claim and non‐claim components and assessment of the Asite → Ib path across levels of motivation to process. Implications for on‐line advertisers are discussed. © 2005 Wiley Periodicals, Inc.
The vaginal pressure profile
Authors: Guaderrama Noelani M. , Nager Charles W. , Liu Jianmin , Pretorius Dolores H. , Mittal Ravinder K. 
Published: February 2005

AbstractAimsTo describe the vaginal pressure profile in asymptomatic nulliparous women.MethodsFourteen nulliparous women without symptoms of anal or urinary incontinence were studied with vaginal manometry. A rapid pull‐through technique utilized a four‐channel water‐perfused catheter on a motor‐driven puller to create a pressure profile for each subject. The profiles were measured with the subject at rest and during a sustained contraction of the levator ani muscle. The individual subject's pressure profiles were averaged to create a composite profile at rest and during squeeze.ResultsThe vaginal pressure profile at rest and during squeeze contains three pressure zones: proximal, mid, and distal. The pressure is highest in the mid pressure zone and was labeled as the vaginal high‐pressure zone. In the vaginal high‐pressure zone, the maximum pressure during squeeze is significantly higher than the maximum pressure at rest (P < 0.05). The length of the high‐pressure zone is longer during squeeze as compared to rest (P < 0.05). The maximum pressures exhibit circumferential asymmetry with the pressures in anterior and posterior directions being significantly higher than those in the lateral directions (P < 0.05).ConclusionsThe vaginal pressure profile is more complex than previously described. Understanding of the vaginal pressure profile is crucial when employing vaginal manometry to assess pelvic floor muscle strength or as a surrogate for intra‐abdominal pressure. © 2005 Wiley‐Liss, Inc.
Effects of local anesthetics on human bladder contractility
Authors: Oh Seung‐June , Paick Sung Hyun , Lim Dae Jung , Lee Eunsik , Lee Sang Eun 
Published: February 2005

AbstractAimsWe investigated the invitro effects of local anesthetics on the contractility of the human bladder.MethodsBy measuring the invitro isometric contractions of human bladder strips, we determined the effects of tetracaine, bupivacaine, lidocaine, and ropivacaine on the basal spontaneous contractions and contractions induced by various stimuli, namely, KCl (60 mM), carbachol (CCh), and electrical field stimulation (EFS). The effect of local anesthetic agents on Ca2+‐independent sustained tonic contraction (SuTC) of the detrusor was also investigated.ResultsLocal anesthetics increased phasic and tonic spontaneous contractile activity dose dependently in the concentration range 1–500 μM, but abolished phasic activity at higher concentrations. Local anesthetic agents inhibited nerve‐mediated contraction (EFS, 0.8 msec) in a concentration‐dependent manner (ropivacaine > tetracaine = bupivacaine > lidocaine), and inhibited non‐nerve mediated contractions induced by KCl, long pulse EFS (direct muscle stimulation, 100 msec), and CCh. Inhibitory potency on non‐nerve mediated contraction was for long pulse EFS: ropivacaine = tetracaine > bupivacaine = lidocaine and for KCl‐ and CCh‐induced contractions: ropivacaine > tetracaine > bupivacaine = lidocaine. Higher concentrations of local anesthetics were needed to inhibit non‐nerve‐mediated bladder contraction than nerve‐mediated contraction. SuTC was suppressed by all local anesthetics concentration dependently.ConclusionsOur study demonstrates that local anesthetics have inhibitory effects on the contraction of human bladder as induced by different stimulants and concentrations. Their effects and differences suggest that they may be considered potentially useful as diagnostic and therapeutic agents for bladder dysfunction. © 2005 Wiley‐Liss, Inc.
Modification of biological parameters after treatment with recombinant factor VIIa in a patient with thrombocytopathy due to storage pool disease
Authors: Langendonck L. , Appel I.M. 
Published: February 2005

AbstractPatients with thrombocytopathy due to storage pool disease mostly suffer from mild bleeding diathesis. However surgical interventions can lead to excess bleeding. We describe how treatment with recombinant factor VIIa (Novoseven®) during a surgical procedure in a boy with SPD leads to an immediate rise in PF‐4, thereby activating factor Xa on the platelet surface, leading to active thrombin generation. Pediatr Blood Cancer 2005;44:676–678. © 2005 Wiley‐Liss, Inc.
Use of alternative and complementary therapies in children with cancer
Authors: Martel Dominique , Bussières Jean‐François , Théorêt Yves , Lebel Denis , Kish Sandra , Moghrabi Albert , Laurier Claudine 
Published: February 2005

AbstractBackground and ObjectivesThe use of complementary and alternative medicines (CAM) is becoming increasingly popular. Although considered beneficial by users, the potential for interaction or substitution with conventional treatment should not be overlooked by health care professionals. It is therefore important to gain insight into the prevalence and the factors related to the use of CAM. To establish the prevalence of use of CAM among children with cancer treated in a large pediatric hospital, describe the profile of use and factors related with use. As a secondary objective we aimed at measuring quality of life of the children aged 5 or more and compare the scores between users and non‐users.MethodsThe study is a cross‐sectional survey of parents whose child was treated at the oncology clinic or ward of Sainte‐Justine Hospital, a large pediatric hospital in Montreal. Data on socio‐demographic variables, the use of CAM and the quality of life was collected through a self‐administered questionnaire. Quality of life was measured with the Child Health Questionnaire CHQ‐PF50, while clinical data was collected from medical records.ResultsA total of 115 patients were recruited and 92 parents completed and returned the questionnaire, resulting in a 80% response rate. According to this survey, 49% of the children used at least one type of CAM and 20% used herbal remedies/homeopathy/vitamins in the 2 months preceding the survey. Most popular CAM were spiritual/mental and physical strategies used by 35 and 33% of children under study, respectively. Only one parent reported having delayed a conventional treatment because of CAM. Although quality of life results must be interpreted with caution, they tend to favor non‐users.ConclusionsCAM is likely to be used by a wide variety of people. In our study, we found that about half of the children with cancer has used CAM in the 2 preceding months. No specific profile of CAM users emerged from this study. The high prevalence of CAM warrants further studies to better understand the reasons and consequences of CAM use particularly on quality of life. Pediatr Blood Cancer 2005;44:660–668. © 2005 Wiley‐Liss, Inc.
Serum creatinine: A surrogate measurement of lean body mass in children with acute lymphoblastic leukemia
Authors: Sala Alessandra , Tarnopolsky Mark , Webber Colin , Norman Geoff , Barr Ronald 
Published: February 2005

AbstractBackgroundSince creatinine is formed almost exclusively in skeletal muscle, the hypothesis of a relationship between serum creatinine (SC) and lean body mass (LBM), determined by dual‐energy X‐ray absorptiometry (DXA), was tested.MethodsTwo cohorts of patients were analyzed: 37 children with acute lymphoblastic leukemia (ALL) and 20 children with primary muscular disorders (PMD). The latter cohort was used as a comparative group. Statistical analyses were performed using linear regressions.ResultsA strong correlation was demonstrated between SC and LBM in the patients with PMD (r = 0.77) and in patients with ALL (r = 0.83 at diagnosis; r = 0.77 on therapy; and r = 0.56 off therapy). The correlation between SC and body size (body mass index) was much weaker (r = 0.38; r = −0.09; r = 0.29 at the successive observations in the ALL cohort and r = 0.05 in the PMD cohort). These data provide support for the initial hypothesis.ConclusionsFurther investigations with a wider variety of diseases are needed for this will allow the consideration of SC as a general surrogate measure of LBM and consequently of nutritional status. Pediatr Blood Cancer 2005; 45:16–19. © 2005 Wiley‐Liss, Inc.
A differential proteome in tumors suppressed by an adenovirus‐based skin patch vaccine encoding human carcinoembryonic antigen
Authors: Huang Chun‐Ming , Shi Zhongkai , DeSilva Tivanka S. , Yamamoto Masato , Van Kampen Kent R. , Elmets Craig A. , Tang De‐chu C. 
Published: February 2005

AbstractWe created an anti‐tumor vaccine by using adenovirus as a vector which contains a cytomegalovirus early promoter‐directed human carcinoembryonic antigen gene (AdCMV‐hCEA). In an attempt to develop the skin patch vaccine, we epicutaneously vaccinated Balb/c mice with AdCMV‐hCEA. After nine weeks post‐immunization, vaccinated mice evoked a robust antibody titer to CEA and demonstrated the capability of suppressing in vivo growth of implanted murine mammay adenocarioma cell line (JC‐hCEA) tumor cells derived from a female Balb/c mouse. Proteomic analysis of the tumor masses in the non‐vaccinated naïve and vaccinated mice reveal that six proteins change their abundance in the tumor mass. The levels of adenylate kinase 1, β‐enolase, creatine kinase M chain, hemoglobin beta chain and prohibitin were statistically increased whereas the level of a creatine kinase fragment, which is undocumented, was decreased in the tumor of vaccinated mice. These proteins may provide a vital link between early‐stage tumor suppression and immune response of skin patch vaccination.
A proteomic approach to studying biogenic amine producing lactic acid bacteria
Authors: Pessione Enrica , Mazzoli Roberto , Giuffrida Maria Gabriella , Lamberti Cristina , Garcia‐Moruno Emilia , Barello Cristina , Conti Amedeo , Giunta Carlo 
Published: February 2005

AbstractAll fermented foods are subject to the risk of biogenic amine contamination. Histamine and tyramine are among the most toxic amines for consumers' health, exerting undesirable effects on the central nervous and vascular systems, but putrescine and cadaverine can also compromise the organoleptic properties of contaminated foods. These compounds are produced by fermenting microbial flora that decarboxylate amino acids to amines. Little is known of the factors which induce biosynthesis of decarboxylating enzymes and/or which modulate their catalytic activity: the accumulation of amines is generally considered to be a mechanism that contrasts an acidic environment and/or that produces metabolic energy through coupling amino acid decarboxylation with electrogenic amino acid/amine antiporters. Two Lactobacillus strains, Lactobacillus sp. 30a (ATCC 33222), and a Lactobacillus sp. strain (w53) isolated from amine‐contaminated wine, carrying genetic determinants for histidine decarboxylase (HDC) and ornithine decarboxylase (ODC), were studied and the influence of some environmental and nutritional parameters on amine production and protein biosynthesis was analyzed through a proteomic approach; this is the first report of a proteomic analysis of amine‐producing bacteria. HDC and ODC biosynthesis were shown to be closely dependent on the presence of high concentrations of free amino acids in the growth medium and to be modulated by the growth phase. The stationary phase and high amounts of free amino acids also strongly induced the biosynthesis of an oligopeptide transport protein belonging to the proteolytic system of Lactic Acid Bacteria. At least two isoforms of glyceraldehyde‐3‐phosphate dehydrogenase, with different Mr, pI and expression profiles, were identified from Lactobacillus sp. w53: the biosynthesis of one isoform, in particular, is apparently repressed by high concentrations of free amino acids. Other proteins were identified from the Lactobacillus proteome, affording a global knowledge of protein biosynthesis modulation during biogenic amine production.
Analysis of the composition of an immunoglobulin E reactive high molecular weight protein complex of peanut extract containing Ara h 1 and Ara h 3/4
Authors: Boldt Andrea , Fortunato Donatella , Conti Amedeo , Petersen Arnd , Ballmer‐Weber Barbara , Lepp Ute , Reese Gerald , Becker Wolf‐Meinhard 
Published: February 2005

AbstractPeanuts (Arachis hypogaea) contain some of the most potent food allergens. In recent years an increasing prevalence of peanut allergies both in children and adults has been observed in the USA and in Europe. In vitro identification and characterization of allergens including those from peanut have been frequently performed by Western blotting. However this method may alter the immunoglobulin E (IgE) antibody reactivity since the proteins are denatured by detergent treatment and/or reduction of disulfide bonds by reducing reagents and does not answer the question how peanut allergens interact with the human digestive apparatus and immune system. Size exclusion chromatography of peanut extract shows that approximately 90% of the total protein content is eluted as one peak in the exclusion volume with a molecular mass of over 200 kDa. The proteins of this fraction were analyzed by blue‐native polyacrylamide gel electrophoresis (PAGE), immunoblotting, two‐dimensional PAGE and Western blotting. A complex of Ara h 1 (Acc. no. P43237), Ara h 3/4 (AAM46958), Ara h 3 (AAC63045), Ara h 4 (AF086821), Gly 1 (AAG01363) and iso‐Ara h 3 (AAT39430) was identified using patients' IgE and allergen‐specific monoclonal antibodies; N‐terminal sequencing and matrix‐assisted laser desorption/ionisation‐time of flight analysis verified these findings. A comparison of the peanut allergen sequences of Ara h 3/4, Ara h 3, Ara h 4 and peanut trypsin inhibitor (AF487543) and the proteins Gly 1 and iso‐Ara h 3, not yet described as allergens, leads to the conclusion that these proteins are isoallergens of each other. It was shown that these isoallergens are post‐translationally cleaved and held together by disulfide bonds in accordance to the 11S plant seed storage proteins signature.
Differential accumulation ofLhcbgene products in thylakoid membranes ofZea maysplants grown under contrasting light and temperature conditions
Authors: Caffarri Stefano , Frigerio Sara , Olivieri Erna , Righetti Pier Giorgio , Bassi Roberto 
Published: February 2005

AbstractIn higher plants many different genes encode Lhcb proteins that belong to a highly conserved protein family. Evolutionary conservation of this genetic redundancy suggests that individual gene products play different roles in light harvesting and photoprotection depending on environmental conditions. We have tested the hypothesis that expression/accumulation of individual light harvesting complex (Lhc) proteins depends on plant growth conditions.Zea maysplants were grown in different temperature (13°Cvs.24°C) and light (highvs.low) conditions. The thylakoid membranes were isolated and fractionated by sucrose gradient and the protein content of the different bands was analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Significant differences were found in the accumulation of both the major light harvesting complex of photosystem II (LHCII) complexes and the minor antenna chlorophyll proteins CP29, CP26 and CP24. In particular, temperature seems to play a major role in driving the expression/accumulation of the different proteins: the LHCII/minor antenna ratio increases with decreasing temperature. The pigment composition and the spectroscopic properties of LHCII complexes isolated from low temperature grown plants are significantly different from those of LHCII purified from high temperature grown plants. Two‐dimensional maps show that different LHCII proteins are accumulated at different levels depending on growth conditions. Moreover the low temperature/high light grown plants show an increased value of nonphotochemical quenching. These results suggest a specific role of different LHCII complexes in the organization of the potosystem II and photoprotection.
Long‐term evaluation of compliance with refuge requirements for Bt cotton
Authors: Carrière Yves , Ellers‐Kirk Christa , Kumar Kamlesh , Heuberger Shannon , Whitlow Mike , Antilla Larry , Dennehy Timothy J , Tabashnik Bruce E 
Published: February 2005

AbstractThe success of the refuge strategy for delaying pest resistance to Bt crops depends on compliance by farmers. However, the accuracy of previous estimates of compliance has been questioned. We have applied a novel approach based on the use of Geographical Information System (GIS) technology to measure compliance with refuge requirements for Bt cotton in six Arizona regions from 1998 to 2003. Although compliance varied among regions, overall compliance was above 88% in five of six years. With the cooperation of farmers, our approach allows precise and economical assessment of compliance with the refuge strategy. Copyright © 2005 Society of Chemical Industry
High‐frequency health data and spline functions
Authors: Martín‐Rodríguez Gloria , Murillo‐Fort Carlos 
Published: February 2005

AbstractSeasonal variations are highly relevant for health service organization. In general, short run movements of medical magnitudes are important features for managers in this field to make adequate decisions. Thus, the analysis of the seasonal pattern in high‐frequency health data is an appealing task. The aim of this paper is to propose procedures that allow the analysis of the seasonal component in this kind of data by means of spline functions embedded into a structural model. In the proposed method, useful adaptions of the traditional spline formulation are developed, and the resulting procedures are capable of capturing periodic variations, whether deterministic or stochastic, in a parsimonious way. Finally, these methodological tools are applied to a series of daily emergency service demand in order to capture simultaneous seasonal variations in which periods are different. Copyright © 2005 John Wiley & Sons, Ltd.
Safety and tolerability of olanzapine compared with other antipsychotics in the treatment of elderly patients with schizophrenia: a naturalistic study
Authors: Ciudad Antonio , Montes José-Manuel , Olivares José-Manuel , Gómez Juan-Carlos ,   the EFESO study group
Published: February 2005

AbstractObjectiveTo evaluate the safety and tolerability of olanzapine in the treatment of elderly patients with schizophrenia.MethodsA total of 135 outpatients with schizophrenia ≥60 years of age were treated with olanzapine (n = 105) or another antipsychotic (n = 30) and followed up for 6 months. Safety measures included the recording of spontaneous adverse events and extrapyramidal symptoms (EPS). Clinical status and effectiveness of the medications were measured using the Clinical Global Impressions-Severity of Illness and the Global Assessment of Function (GAF) scales. Quality of life was assessed by means of the Spanish version of the EuroQol. The Awad scale was applied to evaluate patients’ subjective attitude towards medication.ResultsThe incidence of overall adverse events and EPS was non-significantly lower in patients treated with olanzapine than in patients treated with other antipsychotics. The use of anticholinergic drugs was significantly lower (P = 0.04) in patients treated with olanzapine. Both groups of patients experienced similar improvements in Clinical Global Impressions-Severity and GAF scores. Non-significantly greater improvement in the acceptance of medication occurred at endpoint in olanzapine-treated patients than in control patients as measured by the Awad scale. The improvement in the EuroQol quality of life scale achieved at the end of study did not differ between both treatment groups.ConclusionsResults from this naturalistic study showed that olanzapine was as safe and effective as other antipsychotic drugs in the treatment of elderly patients with schizophrenia.