bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chylothorax after chimeric antigen receptor T cell therapy for relapsed and refractory diffuse large B-cell lymphoma: A case report
Authors: Chen Hsin-Hui , Kuo Cheng-Yi , Ho Ching-Liang , Chen Yeu-Chin 
Published: October 2023

Rationale:
Anti-CD19-targeted chimeric antigen receptor (CAR) T cell therapy is effective in treating relapsed/refractory diffuse large B-cell lymphoma (DLBCL). This therapy is associated with several side effects that can be life-threatening such as cytokine release syndrome (CRS). However, chylothorax associated with CRS after CAR-T therapy has not been reported.

Patient concerns:
A 23-year-old male diagnosed with DLBCL relapsing after autologous peripheral blood stem cell transplantation was treated with anti-CD19-targeted CAR-T cell therapy. After CAR-T cell transfusion, he developed grade 3 CRS includes fever, dyspnea, tachycardia and hypotension. The symptoms of CRS persisted and chest plain film revealed bilateral pleural effusion.

Diagnosis:
Chylothorax was confirmed by the pleural effusion analysis that triglyceride level was 1061 mg/dL. Bacterial and fungal culture of pleural fluid reported no pathogen was detected. Cytological examination of pleural effusion revealed no malignant cells.

Interventions:
The chylothorax resolved after treatment with intravenous administration of tocilizumab.

Outcomes:
On 30-day follow-up, the patient was in stable clinical condition with complete remission of DLBCL on whole-body positron emission tomography scan.

Lessons:
We reported a rare case of CAR-T associated chylothorax in a patient with relapsed and refractory DLBCL. Grade 3 CRS with high interleukin-6 level was presented in our patient. The symptoms of CRS were improved with tocilizumab treatment and the chylothorax resolved later on. It is suggested that high interleukin-6 releases might induce chyle leakage resulting from activations of endothelium and coagulation. Our finding highlights the occurrence of chylothorax during the course of CAR-T cell therapy and the importance of proper monitoring and prompt management of this life-threatening side effect.
Successful robot-assisted laparoscopic resection of pheochromocytoma in a patient with dilated cardiomyopathy: A case report on extremely high-risk anesthesia management
Authors: Huang Long , Wu Jiarui , Lian Baorong , Zhang Daxue , Zhai Yujia , Cao Liming 
Published: October 2023

Rationale:
Anesthetic management during resection of pheochromocytoma is a huge challenge, especially when accompanied by dilated cardiomyopathy (DCM). However, there is a lack of research evidence in this area.

Patient concerns:
A 36-year-old man was admitted with a left retroperitoneal space-occupying lesion, present for 2 years. The patient also had DCM for 2 years. Blood analysis on admission showed elevated levels of norepinephrine and the N-terminus of the brain natriuretic peptide precursor. Abdominal computed tomography revealed a circular shadow in the left adrenal area. Echocardiography showed a cardiac ejection fraction of 31% to 37%, markedly enlarged left atrium and left ventricle, extensive cardiac hypokinesia, and reduced left ventricular diastolic and systolic functions.

Diagnoses:
The preoperative diagnosis was left paraganglioma/pheochromocytoma with DCM.

Interventions:
Multidisciplinary consultation, blood pressure measurements, and volume expansion measurements were performed preoperatively. Invasive arterial blood pressure, central venous pressure, depth of anesthesia, cardiac function, left heart volume, and body temperature were monitored intraoperatively.

Outcomes:
The adrenal pheochromocytoma was successfully removed, and the patient recovered well.

Lessons:
The anesthetic management for adrenal pheochromocytoma resection in adult patients with DCM is extremely high-risk but is evidently not impossible. Adequate preoperative evaluation and preparation, optimization of the anesthesia induction plan, close intraoperative monitoring of cardiac function and hemodynamic changes, and robot-assisted laparoscopic technology are the key success factors. The challenges to anesthetic management may be partly prevented with invasive monitoring techniques and minimally invasive surgery. This case confirms the importance of individual management and multidisciplinary cooperation for a successful outcome.
Effect of adding abdominal bracing to spinal stabilization exercise on lumbar lordosis angle, extensor strength, pain, and function in patients with non-specific chronic low back pain: A prospective randomized pilot study
Authors: Park Han Soo , Park Si Won , Oh Jae-Keun 
Published: October 2023

Trial design:
This study investigated the effect of adding abdominal bracing to spinal stability exercise in patients with chronic low back pain (CLBP). This prospective, randomized pilot study included 67 patients and was conducted at the sports medicine center of a single hospital.

Methods:
The abdominal bracing group (ABBG) underwent spinal stability exercise with abdominal bracing (N = 33), comprising 50 minutes training twice a week for 24 weeks. The control group performed only spinal stability exercise (N = 34) for 50 minutes twice a week for 24 weeks. The ABBG received abdominal bracing training at each session and applied abdominal bracing during the spinal stability exercise. The lumbar lordosis angle (LLA) and spine extensor muscle strength were measured. Spinal flexion angles were measured every 12° from 0° to 72°. The visual analog scale score and Oswestry disability index were measured before treatment and at 12 and 24 weeks after treatment.

Results:
The LLA increased over time in both the groups but was not significantly different between the groups. Spine extensor strength was improved over time in both the groups, and an interactive effect was observed at a spinal flexion angle of 60° and 72°. Pain and function were also improved over time in both the groups, but the effect was stronger in the ABBG than in the control group. In patients with CLBP, spinal stability exercise changed the LLA.

Conclusions:
Although adding abdominal bracing to spinal stability exercise did not affect the changes in the LLA, abdominal bracing improved the spinal extensor strength, pain, and function in patients with CLBP. Therefore, it is recommended to add abdominal bracing to spinal stability exercise to maintain the lordosis angle and to improve CLBP symptoms.
A case report of primary biliary cholangitis combined with ankylosing spondylitis
Authors: Hou Chunfeng , Ren Chunfeng , Luan Luan , Li Shujie 
Published: October 2023

Rationale:
A chronic autoimmune liver disease known as primary biliary cholangitis (PBC) that selectively destructs small intrahepatic biliary epithelial cells and may result in biliary cirrhosis and eventually liver transplantation or death. PBC is associated with various other extrahepatic autoimmune diseases; however, the combination of PBC with ankylosing spondylitis has been rarely reported in the literature. Here, we reported a case of PBC with ankylosing spondylitis to improve our understanding of such coexistence and provide new ideas for the treatment of such patients.

Patient concerns:
A 54-year-old man was presented to the Department of Rheumatology because of an abnormal liver function test for 7 years, chest and back pain for 1 year, and low back pain for 2 months.

Diagnoses:
Primary biliary cholangitis, ankylosing spondylitis, and old pulmonary tuberculosis.

Interventions:
The patient refused to use nonsteroidal anti-inflammatory drugs, conventional synthetic disease-modifying antirheumatic drugs, and biologic disease-modifying antirheumatic drugs; thus, he was treated with methylenediphosphonate (99Tc-MDP) and ursodeoxycholic acid (UDCA).

Outcomes:
The patient achieved remission with UDCA and 99Tc-MDP therapy.

Lessons:
In the treatment of PBC combined with other disorders, the characteristics of different diseases should be considered. The patient reported herein was treated with 99Tc-MDP and UDCA, and his condition improved; thus, we consider 99Tc-MDP to be an effective treatment. Furthermore, in line with the current understanding of the pathogenesis of PBC and ankylosing spondylitis, we hypothesize that interleukin-17 inhibitor is an effective treatment for such patients.
Screening for tuberculosis infection and effectiveness of preventive treatment among people with HIV in low-incidence settings
Authors: van Geuns Dorine , Arts Rob J.W. , de Vries Gerard , Wit Ferdinand W.N.M. , Degtyareva Svetlana Y. , Brown James , Pareek Manish , Lipman Marc , van Crevel Reinout 
Published: October 2023

Objective:
To determine the yield of screening for latent tuberculosis infection (LTBI) among people with HIV (PWH) in low tuberculosis (TB) incidence countries (<10 TB cases per 100 000 persons).

Design:
A systematic review and meta-analysis were performed to assess prevalence and predictive factors of LTBI, rate of TB progression, effect of TB preventive treatment (TPT), and numbers needed to screen (NNS).

Methods:
PubMed and Cochrane Library were searched for studies reporting primary data, excluding studies on active or paediatric TB. We extracted LTBI cases, odds ratios, and TB incidences; pooled estimates using a random-effects model; and used the Newcastle–Ottawa scale for bias.

Results:
In 51 studies with 65 930 PWH, 12% [95% confidence interval (CI) 10–14] had a positive LTBI test, which was strongly associated with origin from a TB-endemic country [odds ratio (OR) 4.7] and exposure to TB (OR 2.9). Without TPT (10 629 PWH), TB incidence was 28/1000 person-years (PY; 95% CI 12–45) for LTBI-test positive versus 4/1000 PY (95% CI 0–7) for LTBI-test-negative individuals. Among 625 PWH (1644 PY) receiving TPT, 15 developed TB (6/1000 PY). An estimated 20 LTBI-positive individuals would need TPT to prevent one case of TB, and numbers NNS to detect LTBI or prevent active TB varied according to a-priori risk of LTBI.

Conclusion:
The relatively high prevalence of LTBI among PWH and the strong correlation with origin from a TB-endemic country support risk-stratified LTBI screening strategies for PWH in low-incidence countries and treating those who test positive.
Mast-Cell Expressed Membrane Protein-1 (MCEMP1) is expressed in classical monocytes and alveolar macrophages in Idiopathic Pulmonary Fibrosis and regulates cell chemotaxis, adhesion, and migration in a TGFβ dependent manner
Authors: Perrot Carole Y. , Karampitsakos Theodoros , Unterman Avraham , Adams Taylor , Marlin Krystin , Arsenault Alyssa , Zhao Amy , Kaminski Naftali , Katlaps Gundars , Patel Kapilkumar , Bandyopadhyay Debabrata , Herazo-Maya Jose D. 
Published: October 2023

AbstractBackgroundMast-Cell Expressed Membrane Protein-1 (MCEMP1) is higher in Idiopathic Pulmonary Fibrosis (IPF) patients with increased risk of death and poor outcomes. Here we seek to establish the mechanistic role of MCEMP1 in pulmonary fibrosis.MethodsMCEMP1 expression was analyzed by single-cell RNA sequencing, immunofluorescence in Peripheral Blood Mononuclear Cells (PBMC) as well as in lung tissues from IPF patients and controls. Chromatin Immunoprecipitation (ChiP) and Proximity Ligation Assay (PLA) were used to study the transcriptional regulation ofMCEMP1. Transient RNA interference and lentivirus transduction were used to knockdown and knock-in MCEMP1 in THP-1 cells to study chemotaxis, adhesion, and migration. Bulk RNA sequencing was used to identify the mechanisms by which MCEMP1 participates in monocyte function. Active RHO pull-down assay was used to validate bulk RNA sequencing results.ResultsWe identified increased MCEMP1 expression in classical monocytes and alveolar macrophages in IPF compared to controls. MCEMP1 was upregulated by TGFβ at the mRNA and protein levels in THP-1. TGFβ-mediated MCEMP1 upregulation results from the cooperation of SMAD3 and SP1 via concomitant binding to SMAD3/SP1cis-regulatory elements within theMCEMP1promoter. In terms of its function, we found that MCEMP1 regulates TGFβ-mediated monocyte chemotaxis, adhesion, and migration. 400 differentially expressed genes were found to increase after TGFβ stimulation of THP-1, further increased in MCEMP1 knock-in cells treated with TGFβ and decreased in MCEMP1 knockdown cells treated with TGFβ. GO annotation analysis of these genes showed enrichment for positive regulation of RHO GTPase activity and signal transduction. While TGFβ enhanced RHO GTPase activity in THP-1 cells, this effect was attenuated following MCEMP1 knockdown.ConclusionMCEMP1 is highly expressed in circulating classical monocytes and alveolar macrophages in IPF. MCEMP1 is regulated by TGFβ and participates in the chemotaxis, adhesion, and migration of circulating monocytes by modulating the effect of TGFβ in RHO activity. Our results suggest that MCEMP1 may regulate the migration and transition of monocytes to monocyte-derived alveolar macrophages during pulmonary fibrosis development and progression.
Developmental and housekeeping transcriptional programs display distinct modes of enhancer-enhancer cooperativity inDrosophila
Authors: Loubiere Vincent , de Almeida Bernardo P. , Pagani Michaela , Stark Alexander 
Published: October 2023

AbstractGenomic enhancers are key transcriptional regulators which, upon the binding of sequence-specific transcription factors, activate their cognate target promoters. Although enhancers have been extensively studied in isolation, a substantial number of genes have more than one simultaneously active enhancer, and it remains unclear how these cooperate to regulate transcription. UsingDrosophilamelanogaster S2 cells as a model, we assay the activities of more than a thousand individual enhancers and a million enhancer pairs towards housekeeping and developmental core promoters with STARR-seq. We report that housekeeping and developmental enhancers show distinct modes of enhancer-enhancer cooperativity: while housekeeping enhancers are additive such that their combined activity mirrors the sum of their individual activities, developmental enhancers are synergistic and follow a multiplicative model of cooperativity. This developmental enhancer synergy is promiscuous and neither depends on the enhancers’ endogenous genomic contexts nor on specific transcription factor motif signatures, but it saturates for the highest levels of enhancer activity. These results have important implications for our understanding of gene-regulation in complex multi-enhancer loci and genomically clustered housekeeping genes, providing a rationale for strong and mild transcriptional effects of mutations within enhancer regions.
CRISPR-Cas tools for simultaneous transcription & translation control in bacteria
Authors: Cardiff Ryan , Faulkner Ian , Beall Juliana , Carothers James , Zalatan Jesse 
Published: October 2023

AbstractRobust control over gene translation at arbitrary mRNA targets is an outstanding challenge in microbial synthetic biology. The development of tools that can regulate translation will greatly expand our ability to precisely control genes across the genome. InE. coli, most genes are contained in multi-gene operons, which are subject to polar effects where targeting one gene for repression leads to silencing of both genes. These effects pose a challenge for independently regulating individual genes in multi-gene operons. Here, we use CRISPR-dCas13 to address this challenge. We find that dCas13-mediated repression exhibits up to 6-fold lower polar effects compared to dCas9. We then show that we can selectively activate single genes in a synthetic multi-gene operon by coupling dCas9 transcriptional activation of an operon with dCas13 translational repression of individual genes within the operon. We also show that dCas13 and dCas9 can be multiplexed for improved biosynthesis of a medically-relevant human milk oligosaccharide. Taken together, our findings suggest that combining transcriptional and translational control can access effects that are difficult to achieve with either mode independently. These combined tools for gene regulation will expand our abilities to precisely engineer bacteria for biotechnology and perform systematic genetic screens.Graphical Abstract
Developing a business intelligence tool for sustainability management
Authors: Chalmeta Ricardo , Ferrer Estevez Maria 
Published: October 2023

PurposeBusiness intelligence (BI) is a combination of computer systems and managerial processes to support decision-making. The balanced scorecard is a kind of business intelligence tool for performance measurement and management control aimed at balancing financial and non-financial as well as short- and long-term measures. The sustainable balanced scorecard is a modification of the original balanced scorecard developed to expressly consider governance, social, environmental and ethical issues, and therefore to allow sustainability concepts to be included within the strategy and the management of the organization. However, although the sustainable balanced scorecard is one of the most suitable tools for integrating sustainability within management, there are few examples of how to develop and implement it which can be used as reference models. To help solve this problem, this paper proposes a methodology for the development of a sustainable balanced scorecard, considering different phases such as planification, analysis, design or computer tool implementation, and describes the findings of three case studies.Design/methodology/approachThe research was conducted using the qualitative multiple-case study method. This made it possible to establish the methodological issues regarding the performance and reporting of this study. Therefore, the research method for the conceptualization and execution of the case studies was divided into seven phases: definition of research goals and questions; proposed theoretical model; identification of units of analysis; case selection; definition of research methods and resources; fieldwork; data collection, classification of information and triangulation; formulation of the enhanced theory, model or methodology; and verification of the rigour and quality of the study.FindingsPaper shows a methodology organized in phases, activities and tasks that allow a sustainable balanced scorecard to be planned, designed, built, computerized and controlled in order to integrate sustainability within the management systems of organizations.Originality/valueThis study contributes to the currently emerging sustainable balanced scorecard literature and practice and, more generally, to research on sustainability measurement and management. The methodology for sustainable balanced scorecard development and implementation showed in this paper contributes to the management and information systems theory because it makes it possible to overcome the shortcomings identified to date: it considers all the sustainability dimensions; it describes all the project life-cycle activities; it encourages stakeholders' participation; and it has been proved to work in real situations.
Risk accumulation assessment method for building construction based on complex network
Authors: Cao Dongqiang , Cheng Lianhua 
Published: October 2023

PurposeIn the evolution process of building construction accidents, there are key nodes of risk change. This paper aims to quickly identify the key nodes and quantitatively assess the node risk. Furthermore, it is essential to propose risk accumulation assessment method of building construction.Design/methodology/approachAuthors analyzed 419 accidents investigation reports on building construction. In total, 39 risk factors were identified by accidents analysis. These risk factors were combined with 245 risk evolution chains. Based on those, Gephi software was used to draw the risk evolution network model for building construction. Topological parameters were applied to interpret the risk evolution network characteristic.FindingsCombining complex network with risk matrix, the standard of quantitative classification of node risk level is formulated. After quantitative analysis of node risk, 7 items of medium-risk node, 3 items of high-risk node and 2 items of higher-risk nodes are determined. The application results show that the system risk of the project is 44.67%, which is the high risk level. It can reflect the actual safety conditions of the project in a more comprehensive way.Research limitations/implicationsThis paper determined the level of node risk only using the node degree and risk matrix. In future research, more node topological parameters that could be applied to node risk, such as clustering coefficients, mesoscopic numbers, centrality, PageRank, etc.Practical implicationsThis article can quantitatively assess the risk accumulation of building construction. It would help safety managers could clarify the system risk status. Moreover, it also contributes to reveal the correspondence between risk accumulation and accident evolution.Originality/valueThis study comprehensively considers the likelihood, consequences and correlation to assess node risk. Based on this, single-node risk and system risk assessment methods of building construction systems were proposed. It provided a promising method and idea for the risk accumulation assessment method of building construction. Moreover, evolution process of node risk is explained from the perspective of risk accumulation.
Antecedents and consequences of corporate social responsibility: a test of multilevel mediating processes
Authors: Peng Jui-Chen , Zhang Kun-shan 
Published: October 2023

PurposeDrawing on social exchange theory and traditional Chinese leadership theory, this research examines employees' perceptions of corporate social responsibility (CSR) using a multilevel mediation model. It also examines the possibility that meaningful work mediates the relationship between perceived CSR and work engagement.Design/methodology/approachSurvey data were collected from 605 employees of 103 departments across 35 companies in Taiwan. Multilevel path models and hypotheses were tested using Mplus structural-equation modeling software.FindingsThe results show that department-level CSR perceptions were positively related to employee-level work engagement and that CSR was a mediating factor between department managers' moral leadership and employee-level work engagement. Additionally, meaningful work played a cross-level mediating role between CSR perceptions and work engagement.Practical implicationsFor organizational managers, these findings imply that enterprises should practice CSR and guide their employees in its correct interpretation. They also reinforce the idea that department leaders should behave ethically, because this will encourage their employees to develop positive perceptions of the company's CSR implementation and thus to be more engaged in their work. Lastly, incorporating CSR programs into training materials and encouraging employees to actively participate in such programs' development, deployment and evaluation should help make work meaningful for employees and further enhance their engagement with it.Originality/valueThis study explains how a cross-level mechanism connects department-level moral leadership to employee-level work engagement in a Chinese cultural context.