bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics of allergic bronchopulmonary mycosis caused by Schizophyllum commune
Authors: Oguma Tsuyoshi , Ishiguro Takashi , Kamei Katsuhiko , Tanaka Jun , Suzuki Junko , Hebisawa Akira , Obase Yasushi , Mukae Hiroshi , Tanosaki Takae , Furusho Shiho , Kurokawa Koji , Watai Kentaro , Matsuse Hiroto , Harada Norihiro , Nakamura Ai , Shibayama Takuo , Baba Rie , Fukunaga Kentaro , Matsumoto Hisako , Ohba Hisano , Sakamoto Susumu , Suzuki Shinko , Tanaka Shintetsu , Yamada Takahiro , Yamasaki Akira , Fukutomi Yuma , Shiraishi Yoshiki , Toyotome Takahito , Fukunaga Koichi , Shimoda Terufumi , Konno Satoshi , Taniguchi Masami , Tomomatsu Katsuyoshi , Okada Naoki , Asano Koichiro ,   Japan ABPM Research Program
Published: January 2024

AbstractBackgroundAllergic bronchopulmonary mycosis (ABPM) is an allergic disease caused by type I and type III hypersensitivity to environmental fungi. Schizophyllum commune, a basidiomycete fungus, is one of the most common fungi that causes non‐Aspergillus ABPM.ObjectiveHerein, we attempted to clarify the clinical characteristics of ABPM caused by S. commune (ABPM‐Sc) compared with those of allergic bronchopulmonary aspergillosis (ABPA).MethodsPatients with ABPM‐Sc or ABPA were recruited from a nationwide survey in Japan, a multicenter cohort, and a fungal database at the Medical Mycology Research Center of Chiba University. The definition of culture‐positive ABPM‐Sc/ABPA is as follows: (1) fulfills five or more of the 10 diagnostic criteria for ABPM proposed by Asano et al., and (2) positive culture of S. commune/Aspergillus spp. in sputum, bronchial lavage fluid, or mucus plugs in the bronchi.ResultsThirty patients with ABPM‐Sc and 46 with ABPA were recruited. Patients with ABPM‐Sc exhibited less severe asthma and presented with better pulmonary function than those with ABPA (p = 0.008–0.03). Central bronchiectasis was more common in ABPM‐Sc than that in ABPA, whereas peripheral lung lesions, including infiltrates/ground‐glass opacities or fibrotic/cystic changes, were less frequent in ABPM‐Sc. Aspergillus fumigatus‐specific immunoglobulin (Ig)E was negative in 10 patients (34%) with ABPM‐Sc, who demonstrated a lower prevalence of asthma and levels of total serum IgE than those with ABPM‐Sc positive for A. fumigatus‐specific IgE or ABPA.ConclusionsClinical characteristics of ABPM‐Sc, especially those negative for A. fumigatus‐specific IgE, differed from those of ABPA.
Vanadium administration ameliorates cortical structural and functional changes in juvenile hydrocephalic mice
Authors: Olopade Funmilayo Eniola , Femi‐Akinlosotu Omowumi Moromoke , Dauda Opeyemi , Obiako Jane , Olopade James Olukayode , Shokunbi Matthew Temitayo 
Published: January 2024

AbstractVanadium is a prevalent neurotoxic transition metal with therapeutic potentials in some neurological conditions. Hydrocephalus poses a major clinical burden in neurological practice in Africa. Its primary treatment (shunting) has complications, including infection and blockage; alternative drug‐based therapies are therefore necessary. This study investigates the function and cytoarchitecture of motor and cerebellar cortices in juvenile hydrocephalic mice following treatment with varying doses of vanadium. Fifty juvenile mice were allocated into five groups (n = 10 each): controls, hydrocephalus‐only, low‐ (0.15 mg/kg), moderate‐ (0.3 mg/kg), and high‐ (3.0 mg/kg) dose vanadium groups. Hydrocephalus was induced by the intracisternal injection of kaolin and sodium metavanadate administered by intraperitoneal injection 72hourly for 28 days. Neurobehavioral tests: open field, hanging wire, and pole tests, were carried out to assess locomotion, muscular strength, and motor coordination, respectively. The cerebral motor and the cerebellar cortices were processed for cresyl violet staining and immunohistochemistry for neurons (NeuN) and astrocytes (glial fibrillary acidic protein). Hydrocephalic mice exhibited body weight loss and behavioral deficits. Horizontal and vertical movements and latency to fall from hanging wire were significantly reduced, while latency to turn and descend the pole were prolonged in hydrocephalic mice, suggesting impaired motor ability; this was improved in vanadium‐treated mice. Increased neuronal count, pyknotic cells, neurodegeneration and reactive astrogliosis were observed in the hydrocephalic mice. These were mostly mitigated in the vanadium‐treated mice, except in the high‐dose group where astrogliosis persisted. These results demonstrate a neuroprotective potential of vanadium administration in hydrocephalus. The molecular basis of these effects needs further exploration.
Real‐time prediction of horizontal drilling pressure based on convolutional Transformer
Authors: Yan Baoyong , Tian Jialin , Wan Jun , Qiu Yu , Chen Weiming 
Published: January 2024

SummaryDuring horizontal drilling operations, real‐time prediction of drilling pressure during the drilling process can help the drilling team cope with the complex and changing working environment downhole, adjust the parameters of the drilling rig promptly, make correct decisions, reduce the probability of drilling accidents, and avoid affecting the duration and cost of the project. This study provides a method for real‐time prediction of the drilling pressure of horizontal drilling rigs. A deep learning model based on a convolutional Transformer is trained for accurate real‐time prediction by extracting real‐time operating data of the horizontal drilling rig from the data acquisition system. The method proposed in this study can be a useful tool to improve the performance of horizontal drilling rigs and can assist the drilling team in operating horizontal drilling rigs. The results of the case study show that: (1) the proposed convolutional Transformer model provides reliable real‐time prediction with an MAE of 0.304 MPa and an RMSE of 0.508 MPa; (2) the proposed method can quickly and accurately predict the trend of drilling pressure change in the next period based on the current change of drilling pressure, and grasp the dynamics of drilling pressure of horizontal drilling rigs in advance. Further research could focus on assisted decision‐making and intelligent optimization to provide solutions for preventing drilling accidents and improving horizontal rig performance based on the prediction.
Slowing Hot Electron Cooling in CdSe Quantum Dots Using Electron‐Rich Exciton‐Delocalizing Ligands
Authors: Baldwin Matthew T. , Lee Changmin , López‐Arteaga Rafael , Weiss Emily A. , Chen Lin X. 
Published: January 2024

AbstractUnderstanding hot carrier dynamics in semiconductor nanocrystals is an important research focus due to their applications in photonics and photovoltaic devices. In this report, we investigated the effects of surface‐bound exciton‐delocalizing ligands (EDLs) on the lifetimes of hot electrons in CdSe quantum dots (QDs). After treatment of CdSe with two different phenylithiocarbamates (PTCs), a class of EDLs, the depletion times of the band‐edge exciton bleach were roughly equivalent as observed through ultrafast transient absorption spectroscopy. However, following the initial ultrafast depletion, the PTC‐treated samples continued to deplete while the untreated CdSe began recovering. Inspection of other transient features – such as the 3rd exciton and hot biexciton – reveal a general trend in which the PTC‐treated samples relax more slowly at short times (<10 ps) when compared with the untreated CdSe. At longer delay times, in the range of nanoseconds, the CdSe+CF3OPTC loses nearly 80 % of its excited state populations, while the CdSe+MeOPTC loses only 20–40 %. We discuss the role that exciton delocalization plays in determining these observed rates as well as how they compare to previous studies. Kinetic differences between the two ligands are attributed to their electron donating/withdrawing abilities and coupling to the CdSe QD. Coherent vibrational wavepacket analysis supports this line of reasoning, showing increased coupling between the exciton and the longitudinal optical (LO) phonon due to increased Coulombic field strength around the hole and electron‐donating MeOPTC. These results indicate that electron‐rich PTCs are especially good candidates for use in QD devices that would make use of hot carriers.
Winter Wheat Response to Winter Pea Cover Crop Under Dryland Cropping
Authors: Gollany Hero 
Published: January 2024

AbstractCover cropping with legumes such as winter peas under dryland winter wheat production systems has several benefits that could improve the resiliency of production systems and agroecosystem services. This article demonstrates the use of winter pea cover crops to increase winter wheat yields in the intermediate (12–16 inch) rainfall zone and to improve drought resiliency. Earn 0.5 CEUs in Crop Management by reading the article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino‐amides blocks and covalent grafting
Authors: Gouyon Jérémie , Clavié Margaux , Raquel Gutiérrez‐Climente , Ngo Giang , Dumy Pascal , Etienne Pascal , Martineau Pierre , Pugnière Martine , Ahmad Mehdi , Subra Gilles , Perrin Catherine , Ladner Yoann 
Published: January 2024

AbstractWe explore a bioinspired approach to design tailored functionalized capillary electrophoresis (CE) surfaces based on covalent grafting for biomolecules analysis. First, the approach aims to overcome well‐known common obstacles in CE protein analysis affecting considerably the CE performance (asymmetry, resolution, and repeatability) such as the unspecific adsorption on fused silica surface and the lack of control of electroosmotic flow (EOF). Then, our approach, which relies on new amino‐amide mimic hybrid precursors synthesized by silylation of amino‐amides (Si–AA) derivatives with 3‐isocyanatopropyltriethoxysilane, aims to recapitulate the diversity of protein–protein interactions (π–π stacking, ionic, Van der Waals…) found in physiological condition (bioinspired approach) to improve the performance of CE protein analysis (electrochromatography). As a proof of concept, these silylated Si–AA (tyrosinamide silylation, serinamide silylation, argininamide silylation, leucinamide silylation, and isoglutamine silylation acid) have been covalently grafted in physiological conditions in different amount on bare fused silica capillary giving rise to a biomimetic coating and allowing both the modulation of EOF and protein–surface interactions. The analytical performances of amino‐amide functionalized capillaries were assessed using lysozyme, cytochrome C and ribonuclease A and compared to traditional capillary coatings poly(ethylene oxide), poly(diallyldimethylammonium chloride), and sodium poly(styrenesulfonate). EOF, protein adsorption rate, protein retention factor k, and selectivity were determined for each coating. All results obtained showed this approach allowed to modulate the EOF, reduce unspecific adsorption, and generate specific interactions with proteins by varying the nature and the amount of Si–AA in the functionalization mixture.
Effect of ferulic acid and p‐coumaric acid on lowering uric acid through network pharmacology and in vitro studies
Authors: Li Jiahui , Liu Weihua , Jiao Wenya , Lian Yunhe , Mi Si , Chitrakar Bimal , Sang Yaxin , Wang Xianghong 
Published: January 2024

AbstractThe occurrence of hyperuricemia is increasing yearly. Based on network pharmacology, this study predicted the molecular targets and signaling pathways of ferulic acid and p‐coumaric acid for improving hyperuricemia. The results were verified through in vitro cell experiments. After enrichment analysis of 11 core targets of ferulic acid and p‐coumaric acid to improve hyperuricemia, the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) signaling pathway was considered as the most significant signaling pathway. In the hyperuricemia cell model, ferulic acid and p‐coumaric acid significantly increased cell viability and decreased the cell uric acid (UA) content. Ferulic acid and p‐coumaric acid significantly regulated the expression of UA transport‐related proteins, namely urate organic anion transporter 1, glucose transporter 9, and adenosine triphosphate‐binding transporter protein G2. Ferulic acid and p‐coumaric acid also downregulated the phosphorylation of PI3K and Akt, which inhibited the PI3K/Akt signaling pathway. This study confirmed that ferulic acid and p‐coumaric acid could regulate UA‐related proteins through the PI3K/Akt pathway and promote UA excretion to alleviate hyperuricemia. The results of this research provided a theoretical basis for further research and development of UA‐lowering products.
Health workforce in the Eastern Mediterranean Region: From COVID‐19 lessons to actions
Authors: Gedik Gulin , Jalal Arooj , Wajid Gohar , Mataria Awad , Hajjeh Rana , Al‐Mandhari Ahmed 
Published: January 2024

AbstractThe COVID‐19 pandemic has had a devastating and unprecedented impact on health and health systems globally leaving an indelible mark on health system infrastructures. The pandemic also clearly demonstrated the critical role of health workers for well‐performing health systems, in particular during emergencies and have prompted the need to undergo a critical re‐evaluation of health systems and health workforce design and implementation. As the year 2023 marks the halfway point of the 2030 Agenda for Sustainable development, the time is pertinent for action by governments and partners to scale up the health workforce to advance towards sustainable developement goal (SDG) 3 on health and well‐being and other health‐related SDGs, building on the lessons from COVID‐19. Therefore, at the 70th session of World Health Organization Regional Committee for Eastern Mediterranean, Member States unanimously adopted a resolution to call for accelerated actions to address health workforce challenges through solidarity, alignment, and synergy of efforts in order to rebuild resilient health systems after the COVID‐19 pandemic.
Immune function assessing of TIM3/CD28‐modified CD19 CAR‐T cells and general CD19 CAR‐T cells through a high‐throughput single‐cell microarray platform
Authors: Wang Chao , Wang Chunhua , Liu Huimin , Zhao Songbo , Qiu Jiaoyan , Li Ping , Liu Zhongqian , Sun Mingyuan , Shao Xiaowei , Wang Yihe , Liang Xiaohong , Gao Lifen , Ma Chunhong , Song Wei , Zhang Yu , Han Lin 
Published: January 2024

AbstractChimeric antigen receptor (CAR) T cells are widely used to treat hematological tumors due to their powerful ability to target and kill cancer cells, of which accurate function evaluation at the single‐cell level is crucial to ensuring the efficacy of diagnosis and treatment. Currently, a universal platform to evaluate the efficacy of immune single cells rapidly, efficiently, and systematically is urgently needed. Here, the cytotoxicity, proliferative potential, and persistence of TIM3/CD28‐modified CD19 CAR‐T cells are evaluated in comparison with ordinary CD19 CAR‐T cells through high‐performance and throughput graphene oxide quantum dot (GOQD)‐based single‐cell microfluidic chips. Overall secretory factor expression, immune‐therapy effect of different effector‐target ratios, spatial immune‐therapy effects, and subgroup type profiling are demonstrated to explicit the immunotherapy effect of TIM3/CD28‐modified CD19 CAR‐T cells. TIM3/CD28‐modified CD19 CAR‐T cells show stronger anti‐tumor ability and maintain excellent immunotherapy effects even at low effector‐target ratios and remote distances. TIM3/CD28 also strengthens the local targeting ability of TIM3/CD28‐modified CD19 CAR‐T. Importantly, TIM3/CD28‐modified CD19 CAR‐T exhibits more distinct Th1/Th2 long‐term persistent and potent killer subgroups, which is very helpful for personalized therapy. Overall, this study provides a valuable approach that can be widely implemented to analyze current CAR‐T combinations and evaluate the function of innovative CAR treatments in the future.
Elucidating the structural, catalytic, and antibacterial traits ofFicus caricaandAzadirachta indicaleaf extract‐mediated synthesis of theAg/CuO/rGOnanocomposite
Authors: Fayyaz Zirwa , Farrukh Muhammad Akhyar , Ul‐Hamid Anwar , Chong Kok‐Keong 
Published: January 2024

AbstractThe present exploration demonstrates the efficient, sustainable, cost‐effective, and environment‐friendly green approach for the synthesis of silver (Ag)‐doped copper oxide (CuO) embedded with reduced graphene oxide (rGO) nanocomposite using the green one‐pot method and the green deposition method. Leaf extracts ofFicus caricaandAzadirachta indicawere used for both methods as reducing and capping agents. The effect of methodology and plant extract was analyzed through different characterization techniques such as UV‐visible spectroscopy, Fourier transform infrared (FT‐IR) spectroscopy, thermogravimetric analysis (TGA), x‐ray diffraction (XRD) analysis, scanning electron microscopy (SEM), high‐resolution transmission electron microscopy (HRTEM). The lowest band gap of 3.0 eV was observed for the Ag/CuO/rGO prepared by the green one‐pot method usingF. carica. The reduction of graphene oxide (GO) and the formation of metal oxide was confirmed through functional group detection using FT‐IR. Calculation of thermodynamic parameters showed that all reactions involved were nonspontaneous and endothermic which shows the stability of nanocomposites. XRD studies revealed the crystallinity, phase purity and small average crystallite size of 32.67 nm. SEM images disclosed that the morphology of the nanocomposites was spherical with agglomeration and rough texture. The particle size of the nanocomposites calculated through HRTEM was found in agreement with the XRD results. The numerous properties of the synthesized nanocomposites enhanced their potential against the degradation of methylene blue, rhodamine B, and ciprofloxacin. The highest percentage degradation of Ag/CuO/rGO was found to be 97%, synthesized using the green one‐pot method withF. caricaagainst ciprofloxacin, which might be due to the lowest band gap, delayed electron–hole pair recombination, and large surface area available. The nanocomposites were also tested against the Gram‐positive and Gram‐negative bacteria.Research HighlightsFacile synthesis of Ag/CuO/rGO nanocomposite using a green one‐pot method and the green deposition method.The lowest band gap of 3.0 eV was observed for nanocomposite prepared by a green one‐pot method usingFicus carica.Least average crystallite size of 32.67 nm was found for nanocomposite prepared by a green one‐pot method usingF. carica.Highest antibacterial and catalytic activity (97%) was obtained against ciprofloxacin with nanocomposite prepared through green one‐pot method usingF. carica.A mechanism of green synthesis is proposed.
Gene therapy for mitochondrial disorders
Authors: Keshavan Nandaki , Minczuk Michal , Viscomi Carlo , Rahman Shamima 
Published: January 2024

AbstractIn this review, we detail the current state of application of gene therapy to primary mitochondrial disorders (PMDs). Recombinant adeno‐associated virus‐based (rAAV) gene replacement approaches for nuclear gene disorders have been undertaken successfully in more than ten preclinical mouse models of PMDs which has been made possible by the development of novel rAAV technologies that achieve more efficient organ targeting. So far, however, the greatest progress has been made for Leber Hereditary Optic Neuropathy, for which phase 3 clinical trials of lenadogene nolparvovec demonstrated efficacy and good tolerability. Other methods of treating mitochondrial DNA (mtDNA) disorders have also had traction, including refinements to nucleases that degrade mtDNA molecules with pathogenic variants, including transcription activator‐like effector nucleases, zinc‐finger nucleases, and meganucleases (mitoARCUS). rAAV‐based approaches have been used successfully to deliver these nucleases in vivo in mice. Exciting developments in CRISPR‐Cas9 gene editing technology have achieved in vivo gene editing in mouse models of PMDs due to nuclear gene defects and new CRISPR‐free gene editing approaches have shown great potential for therapeutic application in mtDNA disorders. We conclude the review by discussing the challenges of translating gene therapy in patients both from the point of view of achieving adequate organ transduction as well as clinical trial design.
A facile synthesis of precursor for the σ‐1 receptor PET radioligand [18F]FTC‐146 and its radiofluorination
Authors: Marešová Anna , Jurášek Michal , Drašar Pavel B. , Dolenský Bohumil , Prokudina Elena A. , Shalgunov Vladimir , Herth Matthias M. , Cumming Paul , Popkov Alexander 
Published: January 2024

The σ‐1 receptor is a non‐opioid transmembrane protein involved in various human pathologies including neurodegenerative diseases, inflammation, and cancer. The previously published ligand [18F]FTC‐146 is among the most promising tools for σ‐1 molecular imaging by positron emission tomography (PET), with a potential for application in clinical diagnostics and research. However, the published six‐ or four‐step synthesis of the tosyl ester precursor for its radiosynthesis is complicated and time‐consuming. Herein, we present a simple one‐step precursor synthesis followed by a one‐step fluorine‐18 labeling procedure that streamlines the preparation of [18F]FTC‐146. Instead of a tosyl‐based precursor, we developed a one‐step synthesis of the precursor analog AM‐16 containing a chloride leaving group for the SN2 reaction with 18F‐fluoride. 18F‐fluorination of AM‐16 led to a moderate decay‐corrected radiochemical yield (RCY = 7.5%) with molar activity (Am) of 45.9 GBq/μmol. Further optimization of this procedure should enable routine radiopharmaceutical production of this promising PET tracer.
Functional knee phenotypes: A helpful classification tool for visualizing potential femoral varus in restricted kinematic alignment total knee arthroplasty in Japan
Authors: Kobayashi Takashi , Kawaguchi Kohei , Goto Kazumi , Suzuki Hayata , Otsu Masayoshi , Michishita Kazuhiko 
Published: January 2024

AbstractPurposeRestricted kinematic alignment (rKA) is a modified technique of kinematically aligned total knee arthroplasty (TKA) within a safe alignment range for long‐term implant survivorship. The purpose of this study was to clarify (1) the distribution of functional knee phenotypes in patients who underwent TKA in Japan and (2) whether the application of this classification results in anatomically neutral alignment after rKA TKA.MethodsOverall, 114 TKA surgeries (mechanical alignment [MA]: 49; rKA: 65) were performed. The joint line orientation angle (JLOA), hip–knee–ankle angle (HKA), femoral mechanical angle (FMA) and tibial mechanical angle (TMA) were obtained. The knees were categorized using a functional knee phenotype classification. Clinical evaluations, including the Knee Injury and Osteoarthritis Outcome, 12‐question Forgotten Joint and Oxford Knee Scores, were performed 3 years postoperatively. Between‐group comparisons were made.ResultsThe most common preoperative functional knee phenotype was VARHKA3° + NEUFMA0° + VARTMA3° (11.4%). In the preoperative population, 51 knees (44.7%) had VARFMA ≥ 3°. Postoperatively, the most common functional knee phenotype was NEUHKA0° + VARFMA3° + VALTMA3° (14 knees, 28.6%) in the MA and NEUHKA0° + NEUFMA0° + NEUTMA0° in the rKA group. The percentage of postoperative JLOA within ±3° from the floor was 27% and 72% in the MA and rKA groups, respectively (p < 0.001). The functional knee phenotype after rKA TKA was neutrally reproduced, and the joint line was more parallel to the ground in the standing position than that of MA. Between‐group differences in clinical outcomes were not significant.ConclusionThe application of functional knee phenotyping to knee osteoarthritis in Japan suggested the presence of racial morphological characteristics. This classification could help better visualize potential femoral varus, contributing to protocol deviation in applying restricted KA TKA.Level of EvidenceLevel IV.