bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensing for survival: specialised regulatory mechanisms of Type III secretion systems in Gram‐negative pathogens
Authors: Manisha Yadav , Srinivasan Mahalashmi , Jobichen Chacko , Rosenshine Ilan , Sivaraman J. 
Published: January 2024

ABSTRACTFor centuries, Gram‐negative pathogens have infected the human population and been responsible for numerous diseases in animals and plants. Despite advancements in therapeutics, Gram‐negative pathogens continue to evolve, with some having developed multi‐drug resistant phenotypes. For the successful control of infections caused by these bacteria, we need to widen our understanding of the mechanisms of host–pathogen interactions. Gram‐negative pathogens utilise an array of effector proteins to hijack the host system to survive within the host environment. These proteins are secreted into the host system via various secretion systems, including the integral Type III secretion system (T3SS). The T3SS spans two bacterial membranes and one host membrane to deliver effector proteins (virulence factors) into the host cell. This multifaceted process has multiple layers of regulation and various checkpoints. In this review, we highlight the multiple strategies adopted by these pathogens to regulate or maintain virulence via the T3SS, encompassing the regulation of small molecules to sense and communicate with the host system, as well as master regulators, gatekeepers, chaperones, and other effectors that recognise successful host contact. Further, we discuss the regulatory links between the T3SS and other systems, like flagella and metabolic pathways including the tricarboxylic acid (TCA) cycle, anaerobic metabolism, and stringent cell response.
ISRIB facilitates the co‐culture of human trophoblast stem cells and embryonic stem cells
Authors: Xia Shuwei , Yu Dainan , Wang Yue , He Beijia , Rong Yin , Chen Shuo , Xiao Zhenyu , Wang Hongmei , Wu Hao , Yan Long 
Published: January 2024

AbstractThe embryo‐like structures (embryoids) constructed by aggregating embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) have provided revolutionary tools for studying the intricate interaction between embryonic and extra‐embryonic tissues during early embryonic development, which has been achieved in mice. However, due to the opposite dependence on some signalling pathways for in vitro culture of human ESCs (hESCs) and TSCs (hTSCs), particularly WNT and TGFβ signalling pathways, which limits the construction of human post‐implantation embryoids by aggregating hESCs and hTSCs. To overcome this challenge, here, by screening 1639 chemicals, we found that an inhibitor of integrated stress response, ISRIB, can replace WNT agonists and TGFβ inhibitors to maintain the stemness and differentiation capacity of hTSCs. Thus, we developed an ISRIB‐dependent in vitro culture medium for hTSCs, namely nTSM. Furthermore, we demonstrated that ISRIB could also maintain the hESC stemness. Using a 3D co‐culture system (hESCs and hTSCs aggregate, ETA), we demonstrated that a 1:1 mixture of hESC culture medium (ESM) and nTSM improved the cell proliferation and organisation of both hESC‐ and hTSC‐compartments and the lumenogenesis of hESC‐compartment in ETAs. Overall, our study provided an ISRIB‐dependent system for co‐culturing hESCs and hTSCs, which facilitated the construction of human embryoids by aggregating hESCs and hTSCs.
Human‐associated genetic landscape of walnuts in the Himalaya: implications for conservation and utilization
Authors: Yan Li‐Jun , Fan Peng‐Zhen , Wambulwa Moses C. , Qi Hai‐Ling , Chen Ying , Wu Zeng‐Yuan , Milne Richard I. , Khan Raees , Luo Ya‐Huang , Gao Lian‐Ming , Shen Shi‐Kang , Rashid Irfan , Khan Shujaul Mulk , Maity Debabrata , Li De‐Zhu , Liu Jie 
Published: January 2024

AbstractAimsA comprehensive understanding of the genetic variation of walnuts (Juglans regia and J. sigillata) in the Himalaya and its potential drivers are essential for the conservation and sustainable utilization of these plant genetic resources. In this study, we aimed to uncover the genetic landscape of walnuts and potential drivers in the Himalaya for better utilization, awareness, sustainable management and conservation of the extant genetic resources of walnuts in the vast Himalayan landscape.LocationThe Himalaya biodiversity hotspot.MethodsA total of 1410 wild or feral walnut trees from 65 populations of J. regia and J. sigillata across four countries in the Himalaya were collected. The genetic diversity, population structure, hybridization and gene flow were thoroughly investigated based on the chloroplast genome and 31 nuclear microsatellite markers. The patterns and drivers of the walnut genetic landscape were further explored.ResultsWe detected three genetic groups of J. regia (JR1, JR2, JR3), one of J. sigillata (JS) and two hybrid types (JR1 × JS and JR3 × JS) of walnut in the Himalaya, with the western Himalaya identified as the genetic diversity hotspot of J. regia. The spatial genetic pattern of the J. regia was significantly influenced by geographic and climatic factors. Human‐mediated dispersal probably promoted the hybridization and gene introgression between J. regia and J. sigillata, which reshaped the genetic landscape of walnut populations in the Himalaya.Main ConclusionsThe extant genetic landscape of walnuts in the Himalaya was driven by natural and anthropogenic forces. Regarding conservation, the western and eastern Himalaya are the genetic reservoir of J. regia and J. sigillata, and hence, pure individuals should be urgently protected from frequent hybridization and introgression. In addition, we propose the utilization of natural hybrid resources coupled with new breeding techniques that combine genomic and phenotypic data.
Similarity of stream insect trait profiles across biogeographic regions
Authors: Kunz Stefan , Kefford Ben J. , Usseglio‐Polatera Philippe , Hawkins Charles P. , Poff N. LeRoy , Akamagwuna Frank , Odume Nelson , Schmidt‐Kloiber Astrid , Graf Wolfram , Metzeling Leon , Matthaei Christoph D. , Phillips Ngaire , Schäfer Ralf B. 
Published: January 2024

AbstractAimHabitat templet theory predicts that the functional niches of species evolved in response to selection pressures imposed by each species' spatial–temporal environment. Consequently, similar environmental conditions should lead to convergence in the biological trait composition of biogeographically independent assemblages. Given their high diversity and ubiquitous occurrence, stream insects represent an ideal group to test convergence. Such an analysis should provide insight into both how spatially variable stream insect traits are and how transferable trait–environment relationships are across large spatial scales. We tested two hypotheses: (1) functional niches of stream insects are similar across Australia, Europe, North America, New Zealand and Southern Africa, and (2) the variability in trait profiles of stream insects is positively related to climatic variability within regions.LocationAustralia, Europe, North America, New Zealand and Southern Africa.MethodsWe used trait datasets from each region to compare functional niches and to delineate groups of insects with similar trait profiles (hereafter, trait profile groups or TPGs) in each region. We identified the traits most important in separating TPGs. Finally, we assessed if trait profile variability between TPGs was associated with climatic variability within each region.ResultsWe found that functional niches of families largely overlapped across the regions examined, except for partial deviations of some Australian families, but that only two trait combinations characterized TPGs across all regions. Feeding mode and respiration traits consistently drove the separation of families into TPGs. The variability of trait profiles slightly increased with increasing climatic variability.Main ConclusionsAlthough our study did not allow to demonstrate mechanisms, it is the first to show large similarities between stream insect functional niches across different biogeographic regions, which might be an indication of their convergence. An important factor shaping stream insect assemblages over these large scales might be climate, indicated by the higher trait profile variation in regions with more diverse climates.
Nitrogen availability and plant functional composition modify biodiversity‐multifunctionality relationships
Authors: Pichon Noémie A. , Cappelli Seraina L. , Soliveres Santiago , Mannall Tosca , Nwe Thu Zar , Hölzel Norbert , Klaus Valentin H. , Kleinebecker Till , Vincent Hugo , Allan Eric 
Published: January 2024

AbstractBiodiversity typically increases multiple ecosystem functions simultaneously (multifunctionality) but variation in the strength and direction of biodiversity effects between studies suggests context dependency. To determine how different factors modulate the diversity effect on multifunctionality, we established a large grassland experiment manipulating plant species richness, resource addition, functional composition (exploitative vs. conservative species), functional diversity and enemy abundance. We measured ten above‐ and belowground functions and calculated ecosystem multifunctionality. Species richness and functional diversity both increased multifunctionality, but their effects were context dependent. Richness increased multifunctionality when communities were assembled with fast‐growing species. This was because slow species were more redundant in their functional effects, whereas different fast species promoted different functions. Functional diversity also increased multifunctionality but this effect was dampened by nitrogen enrichment and enemy presence. Our study suggests that a shift towards fast‐growing communities will not only alter ecosystem functioning but also the strength of biodiversity‐functioning relationships.
Redefine photoprotection: Sun protection beyond sunburn
Authors: van Bodegraven Mirja , Kröger Marius , Zamudio Díaz Daniela F. , Lohan Silke B. , Moritz Rose K. C. , Möller Nadine , Knoblich Chiara , Vogelsang Alexandra , Milinic Zorica , Hallhuber Matthias , Weise Julia M. , Kolbe Ludger , Gallinger Julia , Graupner Cindy , Klose Holger , Ulrich Claas , Meinke Martina C. 
Published: January 2024

AbstractExcessive exposure to ultraviolet (UV) light leads to acute and chronic UV damage and is the main risk factor for the development of skin cancer. In most countries with western lifestyle, the topical application of sunscreens on UV‐exposed skin areas is by far the most frequently used preventive measure against sunburn. Further than preventing sunburns, increasing numbers of consumers are appreciating sunscreens with a medium‐ to high‐level sun protective factor (SPF) as basis for sustainable‐skin ageing or skin cancer prevention programs. However, recent investigations indicate that clinically significant DNA damages as well as a lasting impairment of cutaneous immunosurveillance already occur far below the standard of one minimal erythema dose (MED) sunburn level, which contributes to the current discussion of the clinical value of high‐protective SPF values. Ex vivo investigations on human skin showed that the application of SPF30 reduces DNA damage for a day long sun exposure (24 MED) drastically by about 53% but is significantly surpassed by SPF100 reducing DNA damage by approx. 73%. Further analysis on different SPF protection levels in UV‐exposed cell culture assays focusing on IL‐18, cell vitality and cis/trans‐urocanic acid support these findings. Whereas SPF30 and SPF50+ sunscreens already offer a solid UVB cover for most indications, our results indicate that SPF100 provides significant additional protection against mutagenic (non‐apoptotic‐) DNA damage and functional impairment of the cutaneous immunosurveillance and therefore qualifies as an optimized sunscreen for specifically vulnerable patient groups such as immunosuppressed patients, or skin cancer patients.
Pennies and power: finances, relational power, and marital outcomes
Authors: LeBaron‐Black Ashley B. , Dew Jeffrey P. , Wilmarth Melissa J. , Holmes Erin K. , Serido Joyce , Yorgason Jeremy B. , James Spencer 
Published: January 2024

AbstractObjectiveUsing feminist theory as a lens, we tested whether couple finance behaviors are associated with marital outcomes and whether shared relational power mediates these associations.BackgroundBuilding on a previous study, we sought to understand financial dynamics and relational power (a) in early marriage, (b) using a nationally representative sample, and (c) considering financial deception.MethodWe tested these associations with a nationally representative sample of mixed‐gender U.S. newlyweds (N = 1,654 couples; data are from the CREATE [Couple Relationships and Transition Experiences] project) using an actor–partner interdependence mediation model.ResultsIn support of our hypotheses, we found significant actor and partner associations from financial conflict, financial deception, and unequal influence in financial decision‐making with relational power, marital satisfaction, and marital stability. Additionally, many associations between couple finance variables and marital outcomes were mediated by relational power. We did not find expected associations from employment status and joint/separate bank accounts to relational power, marital satisfaction, or marital stability.ConclusionOur findings support feminist claims suggesting that unequal financial power is linked to unequal relational power, which is in turn associated with lower marital satisfaction and stability.ImplicationsFinancial conflict, financial deception, and unequal financial influence can unbalance relational power and weaken new marriages.
The illusion of balance in the history of the biosphere
Authors: Vermeij Geerat J. 
Published: January 2024

AbstractEarth's surface has been irreversibly altered by the activity of organisms, a process that has accelerated as the power of the biosphere (the rate at which life extracts and deploys energy) has increased over time. This trend is incompatible with the expectation that the inputs to Earth's surface of life's materials from the crust and mantle be matched by export from Earth's surface to long‐term reservoirs. Here, I suggest that the collective activity of organisms has always violated this balance. The biosphere's ability to extract, retain, recycle, and accumulate materials has allowed living biomass to increase and for exports to decrease over very long timescales. This collective metabolism implies a net transfer of materials from the planet's interior to its surface. The combination of metabolic innovations, competition, adaptive evolution, and the establishment of collaborative economic feedback in ecosystems created dynamic ecological stability despite great spatial and temporal heterogeneity in physical and biological inputs and export of nutrients into and out of the biosphere. Models of geochemical cycling must take the fundamental role of living organisms and the evolutionary changes in these roles into account to explain past and future conditions.
High temperatures and low soil moisture synergistically reduce switchgrass yields from marginal field sites and inhibit fermentation
Authors: Chipkar Sarvada , Kahmark Kevin , Bohm Sven , Hussain Mir Zaman , Joshi Leela , Krieg Karleigh M. , Aguado Jacob , Cassidy Jasmine , Lozano Pablo , Garland Kevin , Senyk Andrea , Debrauske Derek J. , Whelan Elizabeth , Davies Morgan , Urban Paul , Robertson G. Philip , Sato Trey K. , Hamilton Stephen K. , Thelen Kurt D. , Ong Rebecca G. 
Published: January 2024

Abstract‘Marginal lands’ are low productivity sites abandoned from agriculture for reasons such as low or high soil water content, challenging topography, or nutrient deficiency. To avoid competition with crop production, cellulosic bioenergy crops have been proposed for cultivation on marginal lands, however on these sites they may be more strongly affected by environmental stresses such as low soil water content. In this study we used rainout shelters to induce low soil moisture on marginal lands and determine the effect of soil water stress on switchgrass growth and the subsequent production of bioethanol. Five marginal land sites that span a latitudinal gradient in Michigan and Wisconsin were planted to switchgrass in 2013 and during the 2018–2021 growing seasons were exposed to reduced precipitation under rainout shelters in comparison to ambient precipitation. The effect of reduced precipitation was related to the environmental conditions at each site and biofuel production metrics (switchgrass biomass yields and composition and ethanol production). During the first year (2018), the rainout shelters were designed with 60% rain exclusion, which did not affect biomass yields compared to ambient conditions at any of the field sites, but decreased switchgrass fermentability at the Wisconsin Central–Hancock site. In subsequent years, the shelters were redesigned to fully exclude rainfall, which led to reduced biomass yields and inhibited fermentation for three sites. When switchgrass was grown in soils with large reductions in moisture and increases in temperature, the potential for biofuel production was significantly reduced, exposing some of the challenges associated with producing biofuels from lignocellulosic biomass grown under drought conditions.
Rodents show darker and redder coloration in warm and rainy environments
Authors: Cerezer Felipe O. , Campos Amanda B. , Dambros Cristian S. , Maestri Renan , Bubadué Jamile M. , Cáceres Nilton C. 
Published: January 2024

AbstractAimAnimal coloration varies in response to environmental conditions. One well‐known principle, Gloger's rule, suggests that warmer and wetter environments lead to more pigmented animals. Yet, the original formulation lacks differentiation between the two primary melanin pigments: eumelanin and pheomelanin. We examined spatial eumelanin and pheomelanin variation to unravel the impact of various ecological factors on pigment deposition, and to assess support for the complex version of Gloger's rule.LocationSouth America.Time PeriodContemporary.Major Taxa StudiedSigmodontine rodents.MethodsWe extracted pelage color data from 231 species and quantified the geographic variation in eu‐ and pheomelanin deposition. We performed linear multiple regression to investigate the influence of temperature, precipitation, predator diversity, and UVA‐B radiance in eumelanin (lightness) and pheomelanin (redness).ResultsOur findings support the original formulations of Gloger's rule. Rodents in warmer and rainier regions, which also entails greater exposure to UV radiation and a diverse range of predators, exhibit darker‐colored pelage. In addition, redder rodents prevail in warmer environments. However, contrary to the rule predictions, we observe a reversal for reddish patterns in relation to precipitation, with rainier regions showcasing more intense red rodents.Main ConclusionsOur study breaks new ground by investigating previously unexplored facets of Gloger's rule in a continental mammalian group. We discovered compelling evidence that darker and redder coloration aligns closely with temperature and rainfall gradients. Although we found support for eumelanin–pelage predictions, expectations for pheomelanin pigmentation were only partially met. Our results might suggest that selective pressures act differently on dark and reddish coloration, revealing that coloration patterns in response to climate are more intricate than previously formulated.
Climate change and mental health and wellbeing: Reflections from a health geography lens
Authors: Martin Gina 
Published: January 2024

AbstractThere is a growing recognition of the importance of research into the effects of climate change on mental health and wellbeing. This paper provides an overview of the pathways through which climate change can affect mental health and wellbeing, highlighting the valuable contribution that health geography can make in this field of study. Given expertise in spatial processes, human‐environment interactions, and diverse research methods, health geographers are well‐equipped to enhance our understanding of the connection between climate change and mental health and wellbeing. The paper proposes two key areas of future focus: (1) exploring the reciprocal relationships between mental health and place, and (2) integrating knowledge from health geography and environmental sustainability. Health geography can play a critical role in developing knowledge to support mitigation strategies and promote mental health and wellbeing in the face of climate change.
“Über dies eigene Ich wieder hinaus”: Stefan Zweig and Adlerian Psychology
Authors: Resch Stephan 
Published: January 2024

AbstractThis article examines the biographical and literary connections between Austrian writer Stefan Zweig and psychologist Alfred Adler. While Zweig's reception of Freudian psychoanalysis has been widely documented by scholarship, the impact of Adler's Individual Psychology on Zweig's works has so far received little attention. The article will first establish the nature and extent of their personal relationship and Zweig's engagement with and reception of Individual Psychology. It will then draw on Zweig's posthumously published novella War er es? (1942) and provide an Adlerian reading of the text. It will be argued that Zweig's focus on Adler in his exile works owes much to his concept of Finalität, which Zweig adopts as a fundamental future‐oriented outlook in much of his later fiction and nonfiction.
A cross‐sectional analysis of medications used by patients reporting alopecia areata on the FDA adverse events reporting system
Authors: Ravipati Advaitaa , Pradeep Tejus , Tosti Antonella 
Published: January 2024

AbstractBackgroundAlopecia areata (AA) is an autoimmune condition that leads to patchy, nonscarring hair loss. Its etiology remains unknown; the condition can be debilitating for patients, impacting their psychosocial wellbeing. Various triggers have been reported, ranging from genetic predisposition and infections to environmental factors. Medications have also been thought to be an inciting factor in AA.MethodsUsing the Food and Drug Administration (FDA) Adverse Events Reporting System (FAERS), all cases reporting AA as an adverse event were used to capture associated medications and patient characteristics.ResultsThere were 1,331 AA cases reported as an adverse event with medication use. Monoclonal antibodies accounted for 6 out of the top 10 drugs associated with the highest number of AA cases. Males were more likely to report AA when taking adalimumab (OR: 1.79, P = 0.04) and dupilumab (OR: 2.56, P = 0.03) compared to females. Individuals between 42 and 64 years old accounted for 46.7% of AA cases. Lastly, females in older age groups showed greater odds of developing AA compared to males (OR: 1.03, P < 0.01).ConclusionsBased on the FAERS, there has been a steady rise in AA cases, and monoclonal antibodies were the most frequently cited medication class tied to AA. With a dearth of literature on triggers and patient demographics, we sought to describe features of AA cases that could increase awareness and be used to improve future clinical outcomes in patients.