bluuebunny/crossref_metadata_2025_split huggingface dataset:
JNK2 silencing lipid nanoparticles for elastic matrix repair
Authors: Dahal Shataakshi , Bastola Suraj , Ramamurthi Anand 
Published: October 2023

AbstractThe over‐expression of c‐Jun N‐terminal kinase (JNK2), a stress activated mitogen kinase, in the aortic wall plays a critical role in the formation and progression of abdominal aortic aneurysm (AAA). This triggers chronic downstream upregulation of elastolytic matrix metalloproteinases (MMPs), MMPs2 and 9 to cause progressive proteolytic breakdown of the wall elastic matrix. We have previously shown that siNRA knockdown of JNK2 gene expression in an AAA culture model stimulates downstream elastin gene expression, elastic fiber formation, crosslinking and reduces elastolytic MMPs2 and 9. Since naked siRNA poorly routes to intracellular targets, has poor stability in blood, and could be potentially toxic and immunogenic, this project is aimed to develop PEGylated lipid nanoparticles (LNPs) for delivery of JNK siRNA and to generate evidence of successful JNK2 knockdown and downstream attenuation of MMP2 gene and protein expressions. LNPs were formulated using thin‐film hydration technique and had the size of 100–200 nm with zeta‐potential ranging between 30 and 40 mV. JNK siRNA loaded PEGylated LNPs successfully knocked down JNK2 in cytokine‐activated rat aneurysmal smooth muscle (EaRASMC) cultures. This resulted in a downstream decrease in MMP2 gene and protein expression and an upward trend in expression of genes for proteins critical for elastic fiber assembly such as elastin (ELN) and lysyl oxidase (LOX). Our result indicates cationic LNPs to be potential carriers for JNK siRNA delivery improving potency for elastin homeostasis required for AAA repair which could possibly provide benefits in preventing the progression of small AAAs.
The timeliness of 10‐K filings, financial performance, and stock returns
Authors: Brooks Li (Lily) Z. , Cheng Yun , Liu Linxiao , Yu Michael D. 
Published: October 2023

AbstractUsing a dataset constructed from the EDGAR database, this study investigates whether the timing of filing 10‐Ks contains useful information to investors. We argue and find that firms with better earnings news are more likely to file their 10‐Ks early. We further show that firms experience earnings increase would accelerate the filing of their 10‐Ks. We explore whether the timeliness measure is useful for predicting future financial performance and stock returns. We find that firms filing 10‐Ks early in the current year are more likely to have better earnings and higher stock returns in future years. Our results are robust to different measures of timeliness, before and after the statutory filing deadline changes, and subsamples of firms with large or small market capitalization.
The paradise fish, an advanced animal model for behavioral genetics and evolutionary developmental biology
Authors: Szabó Nóra , Fodor Erika , Varga Zoltán , Tarján‐Rácz Anita , Szabó Kata , Miklósi Ádám , Varga Máté 
Published: October 2023

AbstractParadise fish (Macropodus opercularis) is an air‐breathing freshwater fish species with a signature labyrinth organ capable of extracting oxygen from the air that helps these fish to survive in hypoxic environments. The appearance of this evolutionary innovation in anabantoids resulted in a rewired circulatory system, but also in the emergence of species‐specific behaviors, such as territorial display, courtship and parental care in the case of the paradise fish. Early zoologists were intrigued by the structure and function of the labyrinth apparatus and a series of detailed descriptive histological studies at the beginning of the 20th century revealed the ontogenesis and function of this specialized system. A few decades later, these fish became the subject of numerous ethological studies, and detailed ethograms of their behavior were constructed. These latter studies also demonstrated a strong genetic component underlying their behavior, but due to lack of adequate molecular tools, the fine genetic dissection of the behavior was not possible at the time. The technological breakthroughs that transformed developmental biology and behavioral genetics in the past decades, however, give us now a unique opportunity to revisit these old questions. Building on the classic descriptive studies, the new methodologies will allow us to follow the development of the labyrinth apparatus at a cellular resolution, reveal the genes involved in this process and also the genetic architecture behind the complex behaviors that we can observe in this species.
Ovarian carcinoma immune‐related microRNA affects the heterogeneity of the endocrine microenvironment and anti‐tumor immune pattern
Authors: Gao Kefei , Wang Qing , Xiang Pan , Xiong Jian , Xu Xindi , Qin Lingshan , Sun Xiang , Fan Yue , Yue Chaomin 
Published: October 2023

AbstractBackgroundThe eighth‐leading cause of cancer‐related mortality and the seventh‐most prevalent malignancy in women globally is ovarian cancer (OV). However, 5‐year survival expectancy after conventional treatment is not good. Therefore, there is an urgent need for novel signatures to guide the designation of therapeutic schemes for OV patients.MethodsWe used univariate Cox analysis to screen hormone secretion regulation axis‐related microRNAs (miRNAs), least absolute shrinkage and selection operator analysis to select candidate miRNAs and multivariate Cox analysis to build the risk model. To evaluate possible route and functional differences, enrichment analysis using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed on the differentially expressed genes (DEGs) across various risk groups. We compared Tumor Immune Dysfunction and Exclusion (TIDE) scores across risk categories by analyzing immune cell infiltration, immune checkpoint gene expression, immunological function and TIDE scores. In the end, we determined the half maximal inhibitory concentration (IC50) of chemotherapy and targeted medicines for individual patients. Cell assays were determined to test the migration of the miRNA‐target genes and western blotting was used to test the correlation of the miRNA‐target genes and the pathways.ResultsWe finally identified hormone secretion regulation axis‐related 13 microRNAs to build a risk model. The validation of observed and anticipated values revealed a fair level of agreement. To evaluate the molecular pathways between various groups in accordance with the GO and KEGG analyses, we then discovered 173 DEGs between distinct risk groups. The risk score was shown to be inversely related to the number of immune cells, including myeloid dendritic, granulocytes, M1 and M2 macrophages, B cells, t‐lymphocytes, and CD4+ and CD8+ cells, suggesting that immune cells are more frequent in the low‐risk group. Immune cell infiltration investigation yielded these results. Finally, we recognized 11 chemotherapeutic drugs and 30 novels targeted drugs on the basis of IC50 between the different risk groups. GJB5 was determined to be the mir‐219 target gene and was identified as promoting the cell cycle process. In addition, hormone secretion regulation axis related miRNAs were reported to affects the heterogeneity of endocrine microenvironment and anti‐tumor immune pattern.ConclusionsIn conclusion, a 13‐miRNA prognostic model was constructed to know the immune status, prognosis, immunotherapeutic response and anti‐tumor drug sensitivity for OV, which provides theoretical guidance for the effective and individualized treatment of OV patients.
Returning to employment following allogeneic hematopoietic stem cell transplant: A major problem among survivors
Authors: Filgueira Luis , Steinberg Amir , Mendonca Rochelle , Lim Seah H. 
Published: October 2023

AbstractQuality of life (QoL) is an important aspect of cancer survivorship. One of the most acute problems that impact survivors in many aspects of activities of daily living and compromise their QoL is the inability to return to employment following successful cancer therapy. This is most prominent among survivors after allogeneic hematopoietic stem cell transplant (allo‐HSCT). More than 50% of the survivors following allo‐HSCT remain unemployed one year after the procedure. This problem extends beyond the initial few years; unemployment rates among those who underwent allo‐HSCT during their childhoods or adolescence have remained high. The inability to return to employment imposes a financial burden. Survivors following allo‐HSCT also experience a multitude of chronic psychosocial complications that may be both contributing and consequential to the inability to return to employment. However, many transplant programs and cancer centers do not have return‐to‐employment programs. In this review paper, we discuss the prevalence of unemployment following allo‐HSCT. We examine the psychosocial symptoms experienced by survivors and how they may affect survivors’ ability to return to employment. Finally, we propose a multi‐disciplinary multi‐pronged occupation‐focused approach to address the complex and inter‐related psychosocial symptoms to help alleviate the problem.
A comparison of the prognostic performance of the Lugano 2014 and RECIL 2017 response criteria in patients with NHL from the phase III GOYA and GALLIUM trials
Authors: Kostakoglu Lale , Martelli Maurizio , Sehn Laurie H. , Davies Andrew , Trněný Marek , Herold Michael , Vitolo Umberto , Hiddemann Wolfgang , Trotman Judith , Knapp Andrea , Mattiello Federico , Nielsen Tina G. , Sahin Deniz , Sellam Gila , Ward Carol , Younes Anas 
Published: October 2023

AbstractThe Lugano 2014 criteria are the standard for response assessment in lymphoma. We compared the prognostic performance of Lugano 2014 and the more recently developed response evaluation criteria in lymphoma (RECIL 2017), which relies primarily on computed tomography and uses unidimensional measurements, in patients with previously untreated diffuse large B‐cell lymphoma (DLBCL) and follicular lymphoma (FL) from the phase III GOYA and GALLIUM trials, respectively. Concordance between responses according to the Lugano 2014 and RECIL 2017 criteria was analyzed. Landmark analyses of progression‐free survival (PFS) and overall survival (OS) by end of treatment (EOT) and end of induction (EOI) response status according to RECIL 2017 and Lugano 2014 criteria, and prognostic value of response at EOT/EOI were also compared. Overall, 1333 patients were included from GOYA and 502 from GALLIUM. Complete response (CR) status according to RECIL 2017 criteria showed high concordance with complete metabolic response (CMR) status by Lugano 2014 criteria in both GOYA (92.5%) and GALLIUM (92.4%). EOT and EOI CR/CMR status by both criteria was highly prognostic for PFS in GOYA (RECIL 2017 [CR]: hazard ratio [HR], 0.35 [95% confidence interval [CI] 0.26–0.46]; Lugano 2014 [CMR]: HR, 0.35 [95% CI 0.26–0.48]; both p < .0001) and GALLIUM (RECIL 2017 [CR]: HR, 0.35 [95% CI 0.23–0.53]; Lugano 2014 [CMR]: HR, 0.21 [95% CI 0.14–0.31]; both p < .0001). In conclusion, response categorization by RECIL 2017 is similar to that by Lugano 2014 criteria, with high concordance observed. Both were prognostic for PFS and OS.
Diastereoselective construction of carbazole‐based spiropyrrolidines and spiropyrrolizidines via cycloaddition reaction
Authors: Varadharajan Panneerselvi , Karikalan Shankar , Kumaresan Prabakaran 
Published: October 2023

AbstractA series of new spiro heterocyclic compounds having carbazole and pyrrolidine units were successfully synthesized through a one‐pot, three component, 1,3‐dipolar cycloaddition reaction in good yields with high diastereoselectivity. Simple reaction, easily accessible chemicals, high yield and diastereoselective formation of monospiropyrrolidine and monospiropyrrolizidine carbazole derivatives in one step are some advantages of this method. The structure of the obtained products was characterized by using spectroscopic techniques.
Quantitative MRI of Gd‐DOTA Accumulation in the Mouse Brain After Intraperitoneal Administration: Validation by Mass Spectrometry
Authors: Tessier Anthony , Ruze Anthony J. , Varlet Isabelle , Laïb Estelle M.H. , Royer Emilien , Bernard Monique , Viola Angèle , Perles‐Barbacaru Teodora‐Adriana 
Published: October 2023

BackgroundIn mice, intraperitoneal (ip) contrast agent (CA) administration is convenient for mapping microvascular parameters over a long‐time window. However, continuous quantitative MRI of CA accumulation in brain over hours is still missing.PurposeTo validate a quantitative time‐resolved MRI technique for mapping the CA kinetics in brain upon ip administration.Study TypeProspective, animal model.Specimen25 C57Bl/6JRj mice underwent MRI.Field Strength/Sequence7‐T, gradient echo sequence.AssessmentGd‐DOTA concentration was monitored by MRI (25 s/repetition) over 135 minutes with (N = 15) and without (N = 10) ip mannitol challenge (5 g/kg). After the final repetition, the brains were sampled to quantify gadolinium by mass spectrometry (MS). Upon manual brain segmentation, the average gadolinium concentration was compared with the MS quantification in transcardially perfused (N = 20) and unperfused (N = 5) mice. Precontrast T1‐maps were acquired in 8 of 25 mice.Statistical TestsOne‐tailed Spearman and Pearson correlation between gadolinium quantification by MRI and by MS, D'Agostino‐Pearson test for normal distribution, Bland–Altman analysis to evaluate the agreement between MRI and MS. Significance was set at P‐value <0.05.ResultsMRI showed that ip administered CA reached the blood compartment (>5 mM) within 10 minutes and accumulated continuously for 2 hours in cerebrospinal fluid (>1 mM) and in brain tissue. The MRI‐derived concentration maps showed interindividual differences in CA accumulation (from 0.47 to 0.81 mM at 2 hours) with a consistent distribution resembling the pathways of the glymphatic system. The average in‐vivo brain concentration 2 hours post‐CA administration correlated significantly (r = 0.8206) with the brain gadolinium quantification by MS for N = 21 paired observations available.Data ConclusionThe presented experimental and imaging protocol may be convenient for monitoring the spatiotemporal pattern of CA uptake and clearance in the mouse brain over 2 hours. The quantification of the CA from the MRI signal in brain is corroborated by MS.Evidence LevelN/ATechnical EfficacyStage 1
The Use of Diffusion Tensor Imaging in the Identification of Acute Rejection and Chronic Allograft Nephropathy After Renal Transplantation
Authors: Jiang Bin , Yu Yixing , Wan Jiayi , Xu Rui , Ma Jiali , Tian Yangyang , Hu Linkun , Wu Peng , Hu Chunhong , Zhu Mo 
Published: October 2023

BackgroundIdentifying the cause of renal allograft dysfunction is important for the clinical management of kidney transplant recipients.PurposeTo evaluate the diagnostic efficiency of diffusion tensor imaging (DTI) for identifying allografts with acute rejection (AR) and chronic allograft nephropathy (CAN).Study TypeProspective.SubjectsSeventy‐seven renal transplant patients (aged 42.5 ± 9.5 years), including 29 patients with well‐functioning stable allografts (Control group), 25 patients diagnosed with acute rejection (AR group), and 23 patients diagnosed with chronic allograft nephropathy (CAN group).Field Strength/Sequence1.5 T/T2‐weighted imaging and DTI.AssessmentThe serum creatinine, proteinuria, pathologic results, and fractional anisotropy (FA) values were obtained and compared among the three groups.Statistical TestOne‐way analysis of variance; correlation analysis; independent‐sample t‐test; intraclass correlation coefficients and receiver operating characteristic curves. Statistical significance was set to a P‐value <0.05.ResultsThe AR and CAN groups presented with significantly elevated serum creatinine as compared with the Control group (191.8 ± 181.0 and 163.1 ± 115.8 μmol/L vs. 82.3 ± 20.9 μmol/L). FA decreased in AR group (cortical/medullary: 0.13 ± 0.02/0.31 ± 0.07) and CAN group (cortical/medullary: 0.11 ± 0.02/0.27 ± 0.06), compared with the Control group (cortical/medullary: 0.15 ± 0.02/0.35 ± 0.05). Cortical FA in the AR group was higher than in the CAN group. The area under the curve (AUC) for identifying AR from normal allografts was 0.756 and 0.744 by cortical FA and medullary FA, respectively. The AUC of cortical FA and medullary FA for differentiating CAN from normal allografts was 0.907 and 0.830, respectively. The AUC of cortical FA and medullary FA for distinguishing AR and CAN from normal allografts was 0.828 and 0.785, respectively. Cortical FA was able to distinguish between AR and CAN with an AUC of 0.728.Data ConclusionDTI was able to detect patients with dysfunctional allografts. Cortical FA can further distinguish between AR and CAN.Evidence Level2Technical EfficacyStage 2
Withdrawal from 3‐Fluoroethamphetamine induces hyperactivity and depression‐like behaviors in male mice
Authors: Jo Cheolmin , Joo Hyejin , Lim Na Young , Park Su‐Jeong , Choi Sun Ok 
Published: October 2023

Abstract3‐Fluoroethamphetamine (3‐FEA) belongs to the amphetamine class of stimulant drugs and functions as a releasing agent for the monoamine neurotransmitters norepinephrine, dopamine, and serotonin. 3‐FEA acts on the central nervous system and elicits physical and mental side effects, such as euphoria, increased heart rate, and excitement. However, little is known about the withdrawal symptoms and behavioral changes induced by 3‐FEA administration. This study aimed to evaluate the short‐term consequences of 3‐FEA administration (twice a day, 7 days, i.p.; 1 and 10 mg/kg) in C57BL/6J mice (male, 7 weeks old) at three behavioral levels following 1–4 days of withdrawal. The evaluation included (1) withdrawal score, (2) hyperactivity (open field [OF], elevated plus maze [EPM], and cliff avoidance [CA] test), and (3) depression‐like behavior (forced‐swim test). In the withdrawal score test, withdrawal behavior increased in all 3‐FEA groups at 16 and 40 h after withdrawal. In the OF, EPM, and CA tests, the 3‐FEA administration group showed significant changes in terms of hyperactivity. In addition, in the forced‐swim test, both the 1 mg/kg and 10 mg/kg 3‐FEA groups showed increased immobility time. These findings indicate that 3‐FEA administration may lead to physical dependence, demonstrated by the withdrawal score increase and significant changes in hyperactivity and depression‐like behavior following repeated administration and drug cessation. In conclusion, this study reveals the adverse consequences of 3‐FEA administration and highlights the need for awareness raising and regulatory action to control the use of this new psychoactive substance.
TGF‐β signaling pathway in spinal cord injury: Mechanisms and therapeutic potential
Authors: Ma Chun‐wei , Wang Zhi‐qiang , Ran Rui , Liao Hai‐yang , Lyu Jia‐yang , Ren Yi , Lei Ze‐yuan , Zhang Hai‐hong 
Published: October 2023

AbstractSpinal cord injury (SCI) is a highly disabling central nervous system injury with a complex pathological process, resulting in severe sensory and motor dysfunction. The current treatment modalities only alleviate its symptoms and cannot effectively intervene or treat its pathological process. Many studies have reported that the transforming growth factor (TGF)‐β signaling pathway plays an important role in neuronal differentiation, growth, survival, and axonal regeneration after central nervous system injury. Furthermore, the TGF‐β signaling pathway has a vital regulatory role in SCI pathophysiology and neural regeneration. Following SCI, regulation of the TGF‐β signaling pathway can suppress inflammation, reduce apoptosis, prevent glial scar formation, and promote neural regeneration. Due to its role in SCI, the TGF‐β signaling pathway could be a potential therapeutic target. This article reported the pathophysiology of SCI, the characteristics of the TGF‐β signaling pathway, the role of the TGF‐β signaling pathway in SCI, and the latest evidence for targeting the TGF‐β signaling pathway for treating SCI. In addition, the limitations and difficulties in TGF‐β signaling pathway research in SCI are discussed, and solutions are provided to address these potential challenges. We hope this will provide a reference for the TGF‐β signaling pathway and SCI research, offering a theoretical basis for targeted therapy of SCI.
Strain and charge doping effect in MoS2 sheets decorated with AgFeO2 particles
Authors: Limaye Mukta Vinayak , Pramanik Monidipa , Badola Shalini , Sahoo Pradosh Kumar , Singh Shashi B. , Saha Surajit , Khare Amit , Thakur Anukul , Dash Adarsh Kumar 
Published: October 2023

AbstractThe presence of local strain in two‐dimensional (2D) materials can alter its properties drastically. When the surface of 2D materials is decorated with some other nanomaterials, local strain develops at the surface which can give rise to synergetic effects. Present study is focused on the explanation of this local strain induced phenomenon observed in 2D materials. In this work, we investigate the evolution of local strain and charge doping in MoS2 decorated with silver ferrite (AgFeO2) particles using Raman spectroscopy. MoS2:AgFeO2 (M:A) samples were fabricated by adding different amount of AgFeO2 particles in MoS2 sheets. The local strain and charge doping for M:A samples were estimated from the observed shifts in the Raman active modes of MoS2, that is,
(∼383.1 cm−1) and
(∼408.1 cm−1). M:A samples also exhibit magnified enhancement in the intensity of
and
modes as compared with bare MoS2. These findings present a new pathway towards engineering surface properties of MoS2.
Effects of 2'‐fucosyllactose on the composition and metabolic activity of intestinal microbiota from piglets after in vitro fermentation
Authors: Zhang Yanan , Ye Yanxin , Guo Jiaqing , Wang Mengting , Li Xuan , Ren Yuting , Zhu Weiyun , Yu Kaifan 
Published: October 2023

AbstractBackgroundAs indigestible carbohydrates, milk oligosaccharides possess various benefits for newborns, mainly through intestinal microbiota, among which 2'‐fucosyllactose (2'‐FL) is the most predominant milk oligosaccharide. However, knowledge about the fermentative characteristics of 2'‐FL in the gut remains limited, especially in the small intestine. The aim of this study is to explore the differential fermentability of 2'‐FL by the small and large intestinal microbiota of piglets using fructo‐oligosaccharide (FOS) and lactose as controls in an in vitro batch fermentation experiment. During fermentation, microbial composition was characterized along with gas production and short‐chain fatty acid production.Results2'‐Fucosyllactose showed differential fermentability in jejunal and colonic fermentation. Compared with the colon, 2'‐FL produced less gas in the jejunum than in the FOS and lactose groups (P < 0.05). Meanwhile, 2'‐FL exhibited a different influence on the microbial composition and metabolism in the jejunum and colon compared with FOS and lactose. In the jejunum, compared with the FOS and lactose groups, the 2'‐FL group showed a higher abundance of Bacteroides, Prevotella, and Blautia, but a lower abundance of Streptococcus and Lactobacillus (P < 0.05), with a higher level of propionate and a lower level of lactate during fermentation (P < 0.05). In the colon, compared with the FOS and lactose groups, 2'‐FL increased the abundance of Blautia, Faecalibacterium, and Lachnospiraceae FCS020, but decreased the abundance of Prevotella_9, Succinivibrio, and Megasphaera (P < 0.05) with an increase in acetate production (P < 0.05).ConclusionOverall, the results suggested that the small intestinal microbiota had the potential to ferment milk oligosaccharides. Meanwhile, in comparison with FOS and lactose, 2′‐FL selectively stimulated the growth of propionate‐producing bacteria in the jejunum and acetate‐producing bacteria in the colon. These results demonstrated the differences in fermentation properties of 2'‐FL by small and large intestinal microbiota and provided new evidence for the application of 2'‐FL in optimizing gut microbiota. © 2023 Society of Chemical Industry.