bluuebunny/crossref_metadata_2025_split huggingface dataset:
A panorama to mine “bioactive X” against progressive deterioration of liver disease: from NAFLD to HCC
Authors: Oh Ki-Kwang , Yoon Sang-Jun , Eom Jung-A , Lee Kyeong Jin , Kwon Goo-Hyun , Kim Dong Joon , Suk Ki-Tae 
Published: November 2023

Abstract
Non-alcoholic fatty liver disease (NAFLD) is implicated in steatohepatitis (NASH), liver cirrhosis (LC) to hepatocellular carcinoma (HCC), sequentially. Herein, our aim was to unravel the nuanced key components (compounds, and targets) to deter the progressive severity concerning hepatocellular diseases. We incorporated rigor bioinformatics and computational screening tools to decode effector(s) against NAFLD, NASH, LC, and HCC. The targets of the four hepatic-diseases were browsed by DisGeNET and OMIM, then, the intersecting targets were identified by Venn diagram. Protein-protein interaction (PPI) networks were constructed on STRING database with the aid of R program. The uppermost target(s) against NAFLD, NASH, LC, and HCC were filtered by degree centrality (DC), and betweenness centrality (BC) value. We utilized the Selleckchem (compound repository website) to retrieve the ligand(s) for the target(s), hereby, confirmed the affinity via molecular docking test (MDT), density functional theory (DFT), and toxicity prediction. The final targets (295) were identified by DisGeNET and OMIM, the core PPI networks comprised 26 nodes, and 248 edges with two key targets (INS, and IL6) in the highest 30% betweenness centrality (BC). The corresponding ligands of PDX1 (transcription factor of INS; one agonist), and IL6 (thirty-two antagonists) were identified by Selleckchem. Molecular docking test (MDT) revealed that PDX1- BRD7552 conformer (-12.1 kcal/mol), and IL6- Forsythoside B (-11.4 kcal/mol) conformer formed most stable complex. In parallel, DFT proposed that BRD7552, and Forsythoside B had significant chemical properties to react the targets, respectively. In conclusion, we decoded causatives of the progressive liver disease with web-based tools in drug repositioning theory. BRD7552 as PDX1 agonist, and Forsythoside B as IL6 antagonist were attributed to synergistic efficacy against NAFLD-derived HCC.
Improved DQN Algorithm for Path Planning of Autonomous Mobile Robots
Authors: Xu XinLi , Cao YunLong , Liu XinYu 
Published: November 2023

Abstract
Deep Q Network (DQN) plays a crucial role in path planning for autonomous mobile robots. The traditional DQN algorithm has problems such as slow convergence speed and being prone to falling into local optima in path planning tasks. To address these issues, this paper proposes an improved DQN algorithm for path planning of autonomous mobile robots. Firstly, the reward function is improved based on heading angle error and distance, and the DHD (distance- heading angle- direction) reward function is designed by combining the motion direction to improve the performance of the algorithm and avoid local optima. Secondly, a weight-sampling learning strategy is designed to increase the utilization rate of training samples and expedite the algorithm's convergence speed. Finally, through comparative simulation experiments, it is verified that the improved DQN algorithm has better performance than traditional DQN and prioritized experience replay.
The study of the antibacterial efficacy and antioxidative activity mediated by exogenous Hydrogen Sulfide against Aeromonas caviae- an antibiotic-resistant organism
Authors: SAHITHYA P S , SINGH SHUBHI , SWAMINATHAN PRIYA 
Published: November 2023

Abstract

Antimicrobial resistance (AMR) is a growing public health threat caused by widespread overuse of antibiotics. Bacteria with antibiotic resistance may acquire resistance genes from soil or water. Endogenous hydrogen sulfide (H2S) production in bacteria confers antibiotic tolerance in many, suggesting a universal defence mechanism against antibiotics. In this study, we isolate soil-based antibiotic-resistant bacteria collected from contaminated areas. An antibiotic-resistant bacteria was identified as non-endogenous-H2S producing, allowing us to examine the effect of exogenous H2S on its resistance mechanism. Therefore, we demonstrated that different classes of antibiotic resistance can be reverted by employing H2S with antibiotics like ampicillin and gentamicin. Methods like Kirby-Bauer Disk Diffusion, Scanning Electron Microscopy and Flow Cytometer analysis were performed to assess the antibacterial activity of H2S with ampicillin and gentamicin. The antioxidative efficiency of H2S was evaluated using the DCFH-DA (ROS) test, as well as lipid peroxidation, and LDH activity. These were further confirmed with enzymatic and non-enzymatic (SOD, CAT, GST and GSH) antioxidant studies. These findings support H2S as antibiotic-potentiator, causing bacterial membrane damage, oxidative stress, and disrupting DNA and proteins. Thus, supplying exogenous H2S can be a good agent for reversal of Antibiotic resistance.
Archaeology meets Environmental Genomics: implementing sedaDNA in the study of the human past
Authors: Özdoğan Kadir Toykan , Gelabert Pere , Hammers Neeke , Altınışık N. Ezgi , Groot Arjen , Plets Gertjan 
Published: November 2023

Abstract

Sedimentary ancient DNA (sedaDNA) has become one of the standard applications in the field of paleogenomics in recent years. It has been used for paleoenvironmental reconstructions, detecting the presence of prehistoric species in the absence of macro remains and even investigating the evolutionary history of a few species. However, its application in archaeology has been limited and primarily focused on humans. This article argues that sedaDNA holds a significant potential in addressing key archaeological questions concerning the origins, lifestyles and environments of past human populations. Our aim is to facilitate the integration of sedaDNA into the standard workflows in archaeology as a transformative tool and thereby unleashing its full potential for studying the human past. Ultimately, we not only underscore the challenges inherent in the sedaDNA field but also provide a research agenda for essential enhancements needed for implementing sedaDNA into the standard workflows of archaeologists.
Cerebellum: Reinforcement Learning Robotic Arm Control System with Hardware Adaptability
Authors: Zhang Shizhuo , Fang Yue , Saini Sujata , Liu Jia , Wu Xibao 
Published: November 2023

Abstract
This paper proposes a meta-learning and reinforcement-learning based control system ”Cerebellum” for robotic arms to address the issue of adapting to hardware parameter changes. By leveraging the con- cept of meta-learning, the system achieves adaptive control by learn- ing from few samples. Experimental results show our ”Cerebellum” reach 90.09% accuracy in a simulation environment with control- ling newly unseen robotic arm. However, challenges remain in hard- ware experiments due to image distortions and Mechanical collide issues. Further research is needed to overcome these challenges.
Implementing risk-based approaches to improve drinking water quality in small water supplies in the Nordic region – barriers and solutions
Authors: Gunnarsdottir Maria J. , Gardarsson Sigurdur M. , Eriksson Magnus , Albrechtsen Hans-Jörgen , Bergkvist Kim Steve Gerlach , Rossi Pekka M. , Matilainen Riikka , Hansen Lisbeth Truelstrup , Jensen Pernille Erland , Maréchal Judith Y. A. , Myrmel Mette , Kalheim Frida Celius , Persson Kenneth M. , Bjerkén August , Bartram Jamie 
Published: November 2023

Abstract
Small water supplies face similar problems worldwide, regardless of ownership or management type. Non-compliance with water quality regulations is more frequent in small supplies than in large ones, as are waterborne disease outbreaks. The new European Union Drinking Water Directive requires risk-based approach (RBA) to secure water safety as is recommended in the World Health Organization's Guidelines for drinking water quality through ‘water safety plans’. This is already in regulation in the Nordic countries, although less used in small supplies. In this research, we explore the challenges, barriers and possible solutions to implementing RBA and improving compliance in small supplies. This was achieved by conducting and analysing interviews with 53 stakeholders from all eight Nordic countries to produce recommendations for action by the different implicated actors. Our findings suggest the centrality of governmental policy, including support for continuous training, provision of simple RBA guidelines and increasing cooperation in the water sector. The Nordic experience reflects global challenges with small water supplies and the trend towards systematic preventive management epitomized in the framework for drinking water safety advocated by the World Health Organization since 2004.
Comparison of Cell-based and Nanoparticle-based Therapeutics in Treating
Atherosclerosis
Authors: Goh Wen Xi , Kok Yih Yih , Wong Chiew Yen 
Published: November 2023

Abstract::
Today, cardiovascular diseases are among the biggest public health threats worldwide. Atherosclerosis,
a chronic inflammatory disease with complex aetiology and pathogenesis, predispose many of these
conditions, including the high mortality rate-causing ischaemic heart disease and stroke. Nevertheless, despite
the alarming prevalence and absolute death rate, established treatments for atherosclerosis are unsatisfactory
in terms of efficacy, safety, and patient acceptance. The rapid advancement of technologies in healthcare research
has paved new treatment approaches, namely cell-based and nanoparticle-based therapies, to overcome
the limitations of conventional therapeutics. This paper examines the different facets of each approach, discusses
their principles, strengths, and weaknesses, analyses the main targeted pathways and their contradictions,
provides insights on current trends as well as highlights any unique mechanisms taken in recent years to
combat the progression of atherosclerosis.
Crocin Ameliorates Diabetic Nephropathy through Regulating Metabolism,
CYP4A11/PPARγ, and TGF-β/Smad Pathways in Mice
Authors: Chen Wei , Su Jinhao , Liu Yubin , Gao Tianmei , Ji Xiaohui , Li Hanzhou , Li Huajun , Wang Yuansong , Zhang Hui , Lv Shuquan 
Published: November 2023

Introduction::
Crocin is one of the main components of Crocus sativus L. and can alleviate oxidative
stress and inflammation in diabetic nephropathy (DN). However, the specific mechanism by which crocin treats
DN still needs to be further elucidated.

Method::
In the present study, a mouse model of DN was first established to investigate the therapeutic effect of
crocin on DN mice. Subsequently, non-targeted metabolomics techniques were used to analyze the mechanisms
of action of crocin in the treatment of DN. The effects of crocin on CYP4A11/PPARγ and TGF-β/Smad pathway
were also investigated.

Result::
Results showed that crocin exhibited significant therapeutic and anti-inflammatory, and anti-oxidative
effects on DN mice. In addition, the non-targeted metabolomics results indicated that crocin treatment affected
several metabolites in kidney. These metabolites were mainly associated with biotin metabolism, riboflavin metabolism,
and arachidonic acid metabolism. Furthermore, crocin treatment upregulated the decreased levels of
CYP4A11 and phosphorylated PPARγ, and reduced the increased levels of TGF-β1 and phosphorylated Smad2/3
in the kidneys of DN mice.

Conclusion::
In conclusion, our study validated the considerable therapeutic, anti-inflammatory, and antioxidative
impacts of crocin on DN mice. The mechanism of crocin treatment may be related to the regulation of
biotin riboflavin and arachidonic acid metabolism, the activation of CYP4A11/PPARγ pathway, and the inhibition
of TGF-β/Smad pathway in the kidney.
Imaging Characteristics and Pathological Analysis of Primary Hepatic
Neuroendocrine Neoplasms
Authors: Li Dan , He Yaqiong , Xie Gongxun , Mao Zhiqun , Liu Peng 
Published: November 2023

Aims::
To investigate the radiological characteristics of the PHNENs on CT and MRI and improve the understanding of the image manifestations and preoperative diagnosis of the disease.

Background::
Primary hepatic neuroendocrine neoplasms (PHNENs) are rare diseases, and most of the relevant studies are case reports. Characterized by no specific clinical symptoms, PHNENs not only have a low preoperative diagnosis rate with great difficulty in early diagnosis but are frequently misdiagnosed as primary hepatic cancer.

Objective::
15 PHNENs patients were enrolled, with 10 cases in the G2 stage and 5 cases in the G3 stage.

Methods::
The imaging and clinicopathological information of 15 patients pathologically diagnosed with PHNENs was retrospectively reviewed.

Results::
The average age of the patients enrolled was 46.14±18.24 years, and the average tumor size was 91.00±61.17 mm. 13 cases showed nodules or masses, 8 cases were located in the periphery of the liver, showing capsule depression and subcapsular effusion signs. CT enhanced scan showed heterogeneous and obvious enhancement in 9 arterial-phase cases , 2 cases in arterial and portal venous phases both saw mild enhancement; the enhancement degree of lesions in the G2 stage in arterial phase was significantly higher than that in the G3 stage. Gd-EOB-DTPA dynamic enhanced MRI was conducted on 3 cases, and scattered lesions with heterogeneous and slight hyperintensity were observed in the hepatobiliary-specific lesions. Image manifestations showed diffuse lesions in 2 cases, with heterogeneous enhancement in arterial phase and decreased enhancement in portal venous phase by the dynamic enhanced scan.

Conclusion::
Central scar, ccapsule depression, and peri-capsular fluid collections in hepatic masses in PHNENs were the imaging characteristics of PHNENs. The CT-enhanced scanning during the arterial phase may provide a certain reference for pathological grading (G2 and G3 grades). Gd-EOB-DTPA-enhanced MRI is helpful for PHNENs diagnosis.
Karanjin, A Promising Bioactive Compound Possessing Anti-cancer Activity against
Experimental Model of Non-small Cell Lung Cancer Cells
Authors: Kumar Gourav , Pandey Dev Mani , Ghosh Manik , Dall'Acqua Stefano , Gupta Rashmi , Tiwari Nishi Prakash , Mohd Siddique Usman , Vishwakrama Leena , Guleri Sunil Kant , Lal Uma Ranjan , Dubey Supriya 
Published: November 2023

Aims:
The aim of this study is to isolate the Millettia pinnata (Karanj) leaf extract for pure compound
with anticancer properties and to study the molecular target of the isolates in non-small cell lung cancer cell lines.

Background:
In our earlier research Millettia pinnata leaf extract has demonstrated potential anticancer activities.
Thus, in pursuit of the bioactive compounds, the most potential active extract from our previous study was purified.
Furthermore, the anticancer properties of the isolated compound karanjin was studied and aimed for apoptosis
and restraining growth

Methods:
A novel method was developed through column chromatography for isolation and purification of the
compound karanjin from leaf chloroform extract. The purified component was then characterised using FTIR,
mass spectrometry, and NMR. An MTT-based cytotoxicity assay was used to analyse cell cytotoxicity, whereas
fluorescence staining was used for apoptosis and reactive oxygen species inhibition quantification. Furthermore,
the real-time PCR assay was used to determine the molecular mechanism of action in cells causing cytotoxicity
induced by karanjin dosing

Results:
The anticancer activity of karanjin in A549 cell line exhibited prominent activity revealing IC50 value of
4.85 μM. Conferring the predicted molecular pathway study, karanjin restrains the proliferation of cancer cells
through apoptosis, which is controlled by extrinsic pathway proteins FAS/FADD/Caspases 8/3/9. Downregulation
of KRAS and dependent gene expression also stopped cell proliferation.

Conclusion:
Karanjin has been identified as a compound with potential effect in non-small cell lung cancer cells.
Molecular mechanism for apoptosis and inhibition of reactive oxygen species induced through H2O2 were observed,
concluding karanjin have medicinal and antioxidant properties.
Research on the Inhibitory Effect of Doxorubicin-loaded Liposomes Targeting GFAP
for Glioma Cells
Authors: Li Qifeng , Xu Jiaming 
Published: November 2023

Background:
Glioma is the most common and devastating brain tumor. In recent years, doxorubicin
(DOX) is one of the drugs used in the treatment of gliomas, but it has side effects and poor clinical outcomes.
Therefore, the delivery of drugs to the tumor site by targeted transport is a new approach to tumor treatment.

Objective:
This study focuses on the anti-tumor effects of GFAP-modified drug-carrying liposomes loaded with
DOX (GFAP-DOX-LPs) on gliomas.

Methods:
GFAP-DOX-LPs were prepared by solvent evaporation method. After characterization analysis of
GFAP-DOX-LPs, the encapsulation efficiency, the drug loading capacity and in vitro release performance were
determined. Then, the MTT method was used to investigate the cytotoxicity and proliferative behavior of U251
and U87 cell lines. After that, flow cytometry was used to investigate the effect of the drug administration group
on tumor cell apoptosis. Eventually, the anti-tumor activity was tested in vivo.

Results:
The average particle size of GFAP-DOX-LPs was determined to be 116.3 ± 6.2 nm, and the average
potential was displayed as 22.8 ± 7.2 mv. Besides, the morphology of the particle indicated a spherical shape. The
encapsulation rate and drug loading were calculated and determined, which were 91.84 ± 0.41% and 9.27 ±
0.55%. In an acidic medium, the DOX release rate reached about 87%. GFAP-DOX-LPs could target glioma cells
with low cytotoxicity and inhibit glioma cell proliferation with high efficiency, resulting in promoting apoptosis.
The anti-tumor effect of GFAP-DOX-LPs was significantly enhanced. At the same time, the number of GFAPpositive
cells in tumor tissues was significantly lower after treatment. Therefore, the overall survival time could
be significantly prolonged.

Conclusion:
The prepared GFAP-DOX-LPs had good targeting and glioma cell inhibition ability. This demonstrated
the promising application of the prepared liposomes in tumor targeting, especially in the field of targeted
drug delivery for the treatment of brain tumor.
Formulation and In-vivo Characterization of 177Lu-tin-colloid as a
Radiosynovectomy Agent
Authors: Mercanoglu Guldem , Alcın Goksel , Ozturkmen Yusuf , Cermik Tevfik 
Published: November 2023

Introduction::
Arthritis is an inflammatory disorder that affects one or more joints of
the body for various reasons, including autoimmune disorders, trauma, or infection. In many
cases, traditional long-term treatment with various drug combinations (NSAIDs, diseasemodifying
antirheumatic drugs, systemic corticosteroids, etc.) can provide relief, but many
joints require additional local treatment. Radiosynovectomy (RSV) is an alternative method to
current treatment options. Both the global supply shortage of 90Y in recent years and the increasing
use of 177Lu-labeled radiopharmaceuticals in the field of nuclear medicine have made it
possible to develop 177Lu-labeled microparticles and test them in small groups as RSV agents.
This study aimed to develop the 177Lu labeled tin colloid formulation and demonstrate its invivo
characterization.

Material and Methods::
Particle size, shape, and labelling efficiency of the four formulations
developed were determined. The formula with the highest labelling efficiency was selected for
further studies. The quality of the formulation was evaluated based on radionuclidic, radiochemical,
and microbial purity. In-vitro stability was evaluated by determining the labelling efficiency.
In-vitro stability was tested in PBS and synovial fluid. The biological characterization was
assessed using SPECT/CT after injecting the formulation into the normal knee joints of the rabbits.

Results::
Aggregated colloidal particles were spherical with a particle size of <5 μm. Labelling
efficiency and radiochemical purity were >95 and 97.65% (Rf=0.2), respectively. The
formulation was stable in vitro for up to 72 hours, both in PBS and synovial fluid. The formulation
was homogeneously distributed in the joint at 0 and 1 hour after injection, and radioactivity-
related involvement and inguinal lymph node involvement due to possible leakage were
not detected in the late period. No pyrogenic/allergic side effects were observed during this period.

Conclusion::
177Lu-tin-colloid was successfully prepared under optimized reaction conditions
with high binding efficiency and radiochemical purity. The radiolabeled colloid was found
to be stable in-vitro both in PBS and synovial fluid at room temperature. Serial PCET/CT
images revealed that the activity was completely retained within the synovial cavity, with
no activity leakage out of the joint until 48 hours after the injection. With the support of
the results from further clinical studies, it may be possible for the formulation to enter clinical
use.
Cancer-specific Nanomedicine Delivery Systems and the Role of the
Tumor Microenvironment: A Critical Linkage
Authors: Dutta Chakraborty Debarupa , Chakraborty Prithviraj 
Published: November 2023

Background::
The tumour microenvironment (TME) affects tumour development in a crucial way. Infinite stromal cells and extracellular matrices located in the tumour form complex tissues. The mature TME of epithelial-derived tumours exhibits common features irrespective of the tumour's anatomical locale. TME cells are subjected to hypoxia, oxidative stress, and acidosis, eliciting an extrinsic extracellular matrix (ECM) adjustment initiating responses by neighbouring stromal and immune cells (triggering angiogenesis and metastasis).

Objective::
This report delivers challenges associated with targeting the TME for therapeutic pur-poses, technological advancement attempts to enhance understanding of the TME, and debate on strategies for intervening in the pro-tumour microenvironment to boost curative benefits.

Conclusion::
Therapeutic targeting of TME has begun as an encouraging approach for cancer treatment owing to its imperative role in regulating tumour progression and modulating treatment response.
Entrepreneurship Education and Locus of Control: Their Influence on Students' Entrepreneurship Interest
Authors: Rozikin Achmad Zainul , Suyati Endang Sri 
Published: November 2023

Abstract A person's interest in entrepreneurship can be influenced by internal factors and external factors. Internal factors include locus of control, while external factors include entrepreneurship education. The purpose of this study was to determine how much influence locus of control and entrepreneurship education had on students' interest in entrepreneurship. The data for the three variables (entrepreneurship education, locus of control, and interest in entrepreneurship) were obtained by distributing questionnaires to the students. The population of this study was 287 students, while the sample was 167 students. The sampling technique used was random sampling technique. The results of this study indicate that interest in entrepreneurship can be influenced by entrepreneurship education and locus of control partially or simultaneously, although the contribution of locus of control is greater than entrepreneurship education.Keywords:Entrepreneurship, Interest In Entrepreneurship, Locus Of Control, Entrepreneurship Education