bluuebunny/crossref_metadata_2025_split huggingface dataset:
USP30 promotes the progression of breast cancer by stabilising Snail
Authors: Sun Kai , Liao Shichong , Yao Xinrui , Yao Feng 
Published: December 2023

AbstractBreast cancer (BC) is the most prevalent tumour in women worldwide. USP30 is a deubiquitinase that has been previously reported to promote tumour progression and lipid synthesis in hepatocellular carcinoma. However, the role of USP30 in breast cancer remains unclear. Therefore, we investigated its biological action and corresponding mechanisms in vitro and in vivo. In our study, we found that USP30 was highly expressed in breast cancer samples and correlated with a poor patient prognosis. Knockdown of USP30 significantly suppressed the proliferation, invasion and migration abilities of BC cells in vitro and tumour growth in vivo, whereas overexpression of USP30 exhibited the opposite effect. Mechanistically, we verified that USP30 interacts with and stabilises Snail to promote its protein expression through deubiquitination by K48-linked polyubiquitin chains and then accelerates the EMT program. More importantly, USP30 reduced the chemosensitivity of BC cells to paclitaxel (PTX). Collectively, these data demonstrate that USP30 promotes the BC cell EMT program by stabilising Snail and attenuating chemosensitivity to PTX and may be a potential therapeutic target in BC.
BETA prime: a first-in-man phase 1 study of AdAPT-001, an armed oncolytic adenovirus for solid tumors
Authors: Conley Anthony P. , Roland Christina L. , Bessudo Alberto , Gastman Brian R. , Villaflor Victoria M. , Larson Christopher , Reid Tony R. , Caroen Scott , Oronsky Bryan , Stirn Meaghan , Williams Jeannie , Burbano Erica , Coyle Angelique , Barve Minal A. , Wagle Naveed , Abrouk Nacer , Kesari Santosh 
Published: December 2023

AbstractAdAPT-001 is an oncolytic adenovirus (OAV) with a transforming growth factor beta (TGF-ß) trap, which neutralizes the immunosuppressive and profibrotic cytokine, TGF-ß. The aim or purpose of this phase 1 study was to assess the safety and tolerability and, secondarily, the efficacy of AdAPT-001 after single intratumoral injection (IT) (Part 1) and multidose IT injection (Part 2) in patients with superficially accessible, advanced refractory solid tumors. Part 1 enrolled 9 patients with a 3 + 3 single dose-escalation safety run-in involving 2.5 × 1011, 5.0 × 1011, 1.0 × 1012 viral particles (vps). No dose-limiting toxicities or treatment-related serious adverse events (SAEs) were seen. In Part 2, a dose-expansion phase, 19 patients received AdAPT-001 at 1.0 × 1012 vps until disease progression according to Response Evaluation Criteria in Solid Tumors or RECIST 1.1. The overall responses to treatment included confirmed partial responses (3), durable stable disease ≥ 6 months (5), and progressive disease (13). AdAPT-001 is well tolerated. Evidence of an anti-tumor effect was seen in both injected and uninjected lesions. The recommended Phase 2 dose was 1.0 × 1012 vp administered by intratumoral injection once every 2 weeks. Combination of AdAPT-001 with a checkpoint inhibition is enrolling.
EP Aquarii: A New Picture of the Circumstellar Envelope
Authors: Nhung Pham T. , Hoai Do T. , Darriulat Pierre , Tuan-Anh Pham , Diep Pham N. , Ngoc Nguyen B. , Thai Tran T. 
Published: December 2023

Abstract
New analyses of earlier ALMA observations of oxygen-rich AGB star EP Aquarii are presented, which complete a previously published analysis and offer a different interpretation of the morpho-kinematics of the circumstellar envelope. The birth of the equatorial density enhancement (EDE) is shown to occur very close to the star where evidence for rotation has been obtained. Close to the star and where outflows have been observed: their interaction with the gas of the nascent EDE is seen to play an important role in the development of the wind and the evolution of its radial velocity from 8 to 10 km s−1 on the polar symmetry axis to ∼2 km s−1 at the equator. It implies complex morpho-kinematics: making reliable interpretations with reasonable confidence is difficult. In particular, it questions an earlier interpretation implying the presence of a white dwarf companion orbiting the star at an angular distance of ∼0.″4 from its center. It proposes instead an interpretation in terms of a standard mass ejection associated with a shock wave leaving a void of emission in its wake. High Doppler velocity wings are seen to consist of two components, the upper velocity end of the global wind, reaching above ±12 km s−1, and an effective line broadening, confined within 200 mas from the center of the star, reaching above ±20 km s−1 and interpreted as caused by the pattern of shock waves resulting from the interaction between stellar pulsation and convective cell partition.
Improving the strategy to identify historical military remains: a literature review and Y-STR meta-analysis
Authors: Mitchell Melinda R , Chaseling Janet , Jones Lee , White Toni , Bernie Andrew , Haupt Larisa M , Griffiths Lyn R , Wright Kirsty M 
Published: December 2023

Abstract

The identification of historical military remains by Unrecovered War Casualties—Army (UWC-A) currently relies on Y-chromosome Short Tandem Repeat (Y-STR) testing when maternal relatives are not available, or when a mitochondrial DNA match does not provide sufficient certainty of identification. However, common Y-STR profiles (using Yfiler™) between sets of remains or families often prevent identification. To resolve these cases, an investigation of additional Y-DNA markers is needed for their potential inclusion into the DNA identification strategy. The number of genetic transmissions between missing soldiers and their living relatives needs to be considered to avoid false exclusions between paternal relatives. Analysis of 236 World War I/II (WWI/II) era pairs of relatives identified up to seven genetic transmissions between WWII soldiers and their living relatives, and nine for WWI. Previous Y-STR meta-analyses were published approximately 10 years ago when rapidly mutating markers were relatively new. This paper reports a contemporary literature review and meta-analysis of 35 studies (which includes 23 studies not previously used in meta-analysis) and 23 commonly used Y-STR’s mutation rates to inform the inclusion of additional loci to UWC-A’s DNA identification strategy. Meta-analysis found mutation data for a given Y-STR locus could be pooled between studies and that the mutation rates were significantly different between some loci (at P &lt; 0.05). Based on this meta-analysis, we have identified two additional markers from PowerPlex® Y23 for potential inclusion in UWC-A’s identification strategy. Further avenues for potential experimental exploration are discussed.

Key points
Study on the influence of spent-catalysts microphysical properties on FCC/asphalt interface interaction
Authors: Kong Lingyun , Li Xinan , He Shengqing , Wu Chufeng , Peng Yi , Wang Hanqing , Shao Qiang , Zhang Allen A 
Published: December 2023

Abstract
This research aims to quantify the interfacial interaction mechanism between the fluid catalytic cracking (FCC) spent catalyst and asphalt. The two types of spent-catalysts, three types of mineral powders, and their bituminous slurries are selected to complete the tests of microscopic morphological, specific surface area, surface energy, fourier transform infrared spectroscopy, specific adhesion work, and interaction parameter C-value for FCC-spent-catalysts in laboratory. The results indicate that: (1), the physical properties of FCC-spent-catalyst compared with mineral powder when the particle size ranging from −2.2 to 5.4 μm between FCC-spent-catalysts and mineral powder, the specific surface area of FCC-spent-catalyst was 100 to 900 fold that of mineral powder, while the alkali value of FCC-spent-catalysts was 2 to 8 fold that of mineral powder; no significant difference was observed in surface energy; (2), the mixture system did not produce new functional groups after FCC-spent-catalyst addition to the asphalt mixture system; (3), the adhesion work of FCC-spent-catalyst was close to that of mineral powder, the specific adhesion work was 74 to 763 fold that of mineral powder when they have the similar particle size; (4), the interaction parameter C-value between FCC-spent-catalyst and asphalt was higher than the interaction between mineral powder and asphalt at identical test temperatures. This study demonstrates that the FCC-spent-catalyst have the potential to improve the in-service performance of the pavement under high-temperature and moisture damage in terms of a larger specific surface area and stronger.
Crosstalk Between Microbiota, Microbial Metabolites, and Interferons in the Inflammatory Bowel Disease Gut
Authors: Vu Vi To Diep , Mahmood Ramsha , Armstrong Heather K , Santer Deanna M 
Published: December 2023

Abstract
With the prevalence of inflammatory bowel diseases (IBD) continuing to rise in Canada and globally, developing improved therapeutics that successfully treat greater percentages of patients with reduced complications is paramount. A better understanding of pertinent immune pathways in IBD will improve our ability to both successfully dampen inflammation and promote gut healing, beyond just inhibiting specific immune proteins; success of combination therapies supports this approach. Interferons (IFNs) are key cytokines that protect mucosal barrier surfaces, and their roles in regulating gut homeostasis and inflammation differ between the three IFN families (type I, II, and III). Interestingly, the gut microbiota and microbial metabolites impact IFN-signaling, yet how this system is impacted in IBD remains unclear. In this review, we discuss the current knowledge of how gut microbiota directly or indirectly impact IFN levels/responses, and what is known about IFNs differentially regulating gut homeostasis and inflammation in animal models or patients with IBD.
What drives OPEC production policy?
Authors: Roeben Volker 
Published: December 2023

Abstract
The Organization of Petroleum Exporting Countries (OPEC) is the international organization with the function of coordinating and unifying the petroleum policies of its member countries. It centrally controls much of the world’s oil supply. While this is generally accepted, it is not well understood how OPEC exercises this broad function, what drives its decision-making. The article closes this gap in the literature through a close study of the published OPEC production policies for the period of 2019–2022. The article finds that internally predicted demand was the primary driver, over external factors that could also have affected production decisions, such as sanctions on OPEC or OPEC+ members, the rise of US shale oil, or climate change mitigation. It follows that consumer states can most effectively influence OPEC petroleum production decision-making by reducing their own oil consumption. The article finally places these findings into a broader context of the law of international organizations, where organizations have a responsibility to exercise their functions within evolving international law. Their decision-making can then be assessed through the three vector-matrix that this article proposes, comprising internal drivers, external factors, and the position of third states.
In-silico analysis of probiotic attributes and safety assessment of probiotic strain Bacillus coagulans BCP92 for human application
Authors: Shaikh Sohel S , Jhala Devendrasinh , Patel Alpesh , Chettiar Shiva shankaran , Ghelani Anjana , Malik Anis , Sengupta Priyajit 
Published: December 2023

Abstract
The whole genome sequence (WGS) of Bacillus coagulans BCP92 is reported along with its genomic analysis of probiotics and safety features. The identification of bacterial strain was carried out using the 16S rDNA sequencing method. Furthermore, gene-related probiotic features, safety assessment (by in vitro and in silico), and genome stability were also studied using the WGS analysis for the possible use of the bacterial strain as a probiotic. From the BLAST analysis, bacterial strain was identified as Bacillus (Heyndrickxia) coagulans. WGS analysis indicated that the genome consists of a 3 475 658 bp and a GC-content of 46.35%. Genome mining of BCP92 revealed that the strain is consist of coding sequences for d-lactate dehydrogenase and l-lactate dehydrogenases, 36 genes involved in fermentation activities, 29 stress-responsive as well as many adhesions related genes. The genome, also possessing genes, is encoded for the synthesis of novel circular bacteriocin. Using an in-silico approach for the bacterial genome study, it was possible to determine that the Bacillus (Heyndrickxia) coagulans strain BCP92 contains genes that are encoded for the probiotic abilities and did not harbour genes that are risk associated, thus confirming the strain’s safety and suitability as a probiotic to be used for human application.
The effect of a ketogenic diet on inflammation-related markers: a systematic review and meta-analysis of randomized controlled trials
Authors: Ji Jiawei , Fotros Danial , Sohouli Mohammad Hassan , Velu Periyannan , Fatahi Somaye , Liu Yinghao 
Published: December 2023

Abstract

Context
Despite the important role of inflammation-related factors on the occurrence of chronic diseases, there is still conflicting evidence about the effects of the ketogenic diet (KD) on these factors.

Objective
In order to obtain a better viewpoint, this study aimed to comprehensively investigate the effects of a KD on inflammation-related markers.

Data Sources
To find pertinent randomized controlled trials up to August 2023, databases including PubMed/Medline, Web of Science, Scopus, Cochrane Library, and Embase were searched.

Data Extraction
This study included all randomized controlled trials investigating the effects of a KD on C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-8, and IL-10 levels. Pooled weighted mean difference (WMD) and 95% confidence intervals (CIs) were achieved by random-effects model analysis for the best estimation of outcomes.

Data Analysis
Forty-four studies were included in this article. The pooled findings showed that a KD has an effect on lowering TNF-α (WMD: −0.32 pg/mL; 95% CI: −0.55, −0.09; P = 0.007) and IL-6 (WMD: −0.27 pg/mL; 95% CI: −0.52, −0.02; P = 0.036) compared with control groups. However, no significant effect was reported for others inflammation marker–related levels. The results of the subgroup analysis showed that, in trials following the KD for ≤8 weeks and in people aged ≤50 years, the reduction in TNF-α levels was significantly higher than in other groups. In addition, in people with a body mass index greater than 30 kg/m2 compared to a body mass index ≤30 kg/m2, IL-6 levels decreased to a greater extent after receiving the KD.

Conclusions
Consequently, adherence to a KD appears to improve some markers associated with inflammation, including TNF-α and IL-6.
Changes in tissue protein N-glycosylation and associated molecular signature occur in the human Parkinsonian brain in a region-specific manner
Authors: Rebelo Ana Lúcia , Drake Richard R , Marchetti-Deschmann Martina , Saldova Radka , Pandit Abhay 
Published: December 2023

Abstract
Parkinson's disease (PD) associated state of neuroinflammation due to the aggregation of aberrant proteins is widely reported. One type of post-translational modification involved in protein stability is glycosylation. Here, we aimed to characterize the human Parkinsonian nigro-striatal N-glycome, and related transcriptome/proteome, and its correlation with endoplasmic reticulum (ER) stress and unfolded protein response (UPR), providing a comprehensive characterization of the PD molecular signature. Significant changes were seen upon a PD: a 3% increase in sialylation and 5% increase in fucosylation in both regions, and a 2% increase in oligomannosylated N-glycans in the substantia nigra. In the latter, a decrease in the mRNA expression of sialidases and an upregulation in the UPR pathway were also seen. To show the correlation between these, we also describe a small in vitro study where changes in specific glycosylation trait enzymes (inhibition of sialyltransferases) led to impairments in cell mitochondrial activity, changes in glyco-profile, and upregulation in UPR pathways. This complete characterization of the human nigro-striatal N-glycome provides an insight into the glycomic profile of PD through a transversal approach while combining the other PD “omics” pieces, which can potentially assist in the development of glyco-focused therapeutics.
Ultrasound-based detection of inflammatory changes for early diagnosis and risk model construction of psoriatic arthritis
Authors: Wang Yiyi , Liu Nuozhou , Zhang Lingyan , Yang Min , Xiao Yue , Li Furong , Hu Hongxiang , Qiu Li , Li Wei 
Published: December 2023

Abstract

Objectives
PsA is the most prevalent coexisting condition associated with psoriasis. Early-stage PsA patients always present unspecific and subtle clinical manifestations causing delayed diagnosis and leading to unfavourable health outcomes. The application of US enables precise identification of inflammatory changes in musculoskeletal structures. Hence, we constructed US models to aid early diagnosis of PsA.

Methods
This was a cross-sectional study carried out in the Department of Dermatology at West China Hospital (October 2018–April 2021). All participants underwent thorough US examinations. Participants were classified into the under 45 group (18 ≤ age ≤ 45 years) and over 45 (age &gt;45 years) group and then randomly grouped into derivation and test cohort (7:3). Univariable logistic regression, least absolute shrinkage and selection operator, and multivariable logistic regression visualized by nomogram were conducted in order. Receiver operating characteristic (ROC), calibration curve, decision curve analysis (DCA) and clinical impact curve analysis (CICA) were performed for model verification.

Results
A total of 1256 participants were included, with 767 participants in the under 45 group and 489 in the over 45 group. Eleven and 16 independent ultrasonic variables were finally selected to construct the under 45 and over 45 model with the area under the ROC of 0.83 (95% CI 0.78–0.87) and 0.83 (95% CI 0.78–0.88) in derivation cohort, respectively. The DCA and CICA analyses showed good clinical utility of the two models.

Conclusion
The implementation of the US models could streamline the diagnostic process for PsA in psoriasis patients, leading to expedited evaluations while maintaining diagnostic accuracy.
Ammonium perchlorate: serum dosimetry, neurotoxicity, and resilience of the neonatal rat thyroid system
Authors: Gilbert Mary E , Hassan Iman , O’Shaughnessy Katherine L , Wood Carmen , Stoker Tammy E , Riutta Cal , Ford Jermaine L 
Published: December 2023

Abstract
The environmental contaminant perchlorate impairs the synthesis of thyroid hormones by reducing iodine uptake into the thyroid gland. Despite this known action, moderate doses of perchlorate do not significantly alter serum thyroid hormone in rat pups born to exposed dams. We examined perchlorate dosimetry and responsivity of the thyroid gland and brain in offspring following maternal exposure to perchlorate. Pregnant rat dams were delivered perchlorate in drinking water (0, 30, 100, 300, 1000 ppm) from gestational day 6 to postnatal day (PN) 21. Perchlorate was present in the placenta, milk, and serum, the latter declining in pups over the course of lactation. Serum and brain thyroid hormone were reduced in pups at birth but recovered to control levels by PN2. Dramatic upregulation of Nis was observed in the thyroid gland of the exposed pup. Despite the return of serum thyroid hormone to control levels by PN2, expression of several TH-responsive genes was altered in the PN14 pup brain. Contextual fear learning was unimpaired in the adults, supporting previous reports. Declining levels of serum perchlorate and a profound upregulation of Nis gene expression in the thyroid gland are consistent with the rapid return to the euthyroid state in the neonate. However, despite this recovery, thyroid hormone insufficiencies in serum and brain beginning in utero and present at birth appear sufficient to alter TH action in the fetus and subsequent trajectory of brain development. Biomarkers of that altered trajectory remain in the brain of the neonate, demonstrating that perchlorate is not devoid of effects on the developing brain.
Bp-miR408a participates in osmotic and salt stress responses by regulating BpBCP1 in Betula platyphylla
Authors: Liu Zhongyuan , Xu Ruiting , Fan Yingbo , Dong Wenfang , Han Yating , Xie Qingjun , Li Jinghang , Liu Baichao , Wang Chao , Wang Yucheng , Fu Yujie , Gao Caiqiu 
Published: December 2023

Abstract
The microRNAs, which are small RNAs of 18–25 nt in length, act as key regulatory factors in posttranscriptional gene expression during plant growth and development. However, little is known about their regulatory roles in response to stressful environments in birch (Betula platyphylla). Here, we characterized and further explored miRNAs from osmotic- and salt-stressed birch. Our analysis revealed a total of 190 microRNA (miRNA) sequences, which were classified into 180 conserved miRNAs and 10 predicted novel miRNAs based on sequence homology. Furthermore, we identified Bp-miR408a under osmotic and salt stress and elucidated its role in osmotic and salt stress responses in birch. Notably, under osmotic and salt stress, Bp-miR408a contributed to osmotic and salt tolerance sensitivity by mediating various physiological changes, such as increases in reactive oxygen species accumulation, osmoregulatory substance contents and Na+ accumulation. Additionally, molecular analysis provided evidence of the in vivo targeting of BpBCP1 (blue copper protein) transcripts by Bp-miR408a. The overexpression of BpBCP1 in birch enhanced osmotic and salt tolerance by increasing the antioxidant enzyme activity, maintaining cellular ion homeostasis and decreasing lipid peroxidation and cell death. Thus, we reveal a Bp-miR408a–BpBCP1 regulatory module that mediates osmotic and salt stress responses in birch.