bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current and future potential distribution of Culex (Melanoconion) (Diptera: Culicidae) of public health interest in the Neotropics
Authors: Laurito Magdalena , Arias-Alzate Andrés 
Published: February 2024

Abstract
Anthropogenic activities are altering ecosystem stability and climate worldwide, which is disturbing and shifting arbovirus vector distributions. Although the overall geographic range of some epidemiologically important species is recognized, the spatiotemporal variation for other species in the context of climate change remains poorly understood. Here we predict the current potential distribution of 9 species of Culex (Melanoconion) based on an ecological niche modeling (ENM) approach and assess spatiotemporal variation in future climate change in the Neotropics. The most important environmental predictors were the mean temperature of the warmest season (27 °C), precipitation during the driest month (50 mm), and precipitation during the warmest season (&gt;200 mm). The best current model for each species was transferred to the future general circulation model IPSL-CM6A-LR, using 2 shared socioeconomic pathway scenarios (ssp1-2.6, ssp5-8.5). Under both scenarios of climatic change, an expansion of suitable areas can be observed followed by a strong reduction for the medium–long future under the worst scenario. The multivariate environmental similarity surface analysis indicated future novel climates outside the current range. However, none of the species would occur in those areas. Even if many challenges remain in improving methods for forecasting species responses to global climate change and arbovirus transmission, ENM has strong potential to be applied to the geographic characterization of these systems. Our study can be used for the monitoring of Culex (Melanoconion) species populations and their associated arboviruses, contributing to develop region-specific public health surveillance programs.
YHWH as a Woman in Labour: The Controlled Pregnant Female Body in Labour
Authors: Langton Karen 
Published: February 2024

Abstract
In the Hebrew Bible, there are 14 similes of a woman in labour. Thirteen of these similes describe a crisis in which the uncontrollable pregnant female body is used to depict death, crisis, destruction, and even annihilation. However, when YHWH is like a woman in labour (Isa. 42:14), the pregnant body in labour is described as controlled, restrained, and, one could say, desirous. Rather than interpret this image in light of the simile of YHWH as a warrior in the preceding verse (Isa. 42:13), I argue that the image is purposefully in opposition. Rather than a display of warrior-like strength, YHWH as a woman in labour is calm and controlled. To support my argument, I look at the simile through the lens of the experience of the female body. Using examples of women in labour in ancient texts and current women’s narratives, I show how ancient writers draw upon the pregnant female bodily experience of being in labour to communicate YHWH’s complete control over humanity’s relationship with him.
Exploring the origin of the distance bimodality of stars in the periphery of the Small Magellanic Cloud with APOGEE and Gaia
Authors: Almeida Andres , Majewski Steven R , Nidever David L , Olsen Knut A G , Monachesi Antonela , Kallivayalil Nitya , Hasselquist Sten , Choi Yumi , Povick Joshua T , Wilson John C , Geisler Doug , Lane Richard R , Nitschelm Christian , Sobeck Jennifer S , Stringfellow Guy S 
Published: February 2024

ABSTRACT
The Magellanic Cloud system represents a unique laboratory for study of both interacting dwarf galaxies and the ongoing process of the formation of the Milky Way and its halo. We focus on one aspect of this complex, three-body interaction – the dynamical perturbation of the Small Magellanic Cloud (SMC) by the Large Magellanic Cloud (LMC), and specifically potential tidal effects on the SMC’s eastern side. Using Gaia astrometry and the precise radial velocities (RVs) and multielement chemical abundances from Apache Point Observatory Galactic Evolution Experiment (APOGEE-2) Data Release 17, we explore the well-known distance bimodality on the eastern side of the SMC. Through estimated stellar distances, proper motions, and RVs, we characterize the kinematics of the two populations in the bimodality and compare their properties with those of SMC populations elsewhere. Moreover, while all regions explored by APOGEE seem to show a single chemical enrichment history, the metallicity distribution function (MDF), of the ‘far’ stars on the eastern periphery of the SMC is found to resemble that for the more metal-poor fields of the western periphery, whereas the MDF for the ‘near’ stars on the eastern periphery resembles that for stars in the SMC Centre. The closer eastern periphery stars also show RVs (corrected for SMC rotation and bulk motion) that are, on average, approaching us relative to all other SMC populations sampled. We interpret these trends as evidence that the near stars on the eastern side of the SMC represent material pulled out of the central SMC as part of its tidal interaction with the LMC.
Probing the magnetic field and dust grain properties of two dark clouds L1495 and L1498 through photopolarimetry
Authors: Choudhury G B , Goswami B , Das H S , Medhi B J , Pandey J C 
Published: February 2024

ABSTRACT
We present the R-band polarimetry of two low-latitude Lynd’s clouds L1495 and L1498 acquired with AIMPOL at Aryabhatta Research Institute of Observational Sciences, Nainital, India. The estimated polarization across the regions of L1495 and L1498 is typically in the range of ∼1–5 per cent and the position angle is typically in the range of ∼100°–137° from which we infer the morphologies of the corresponding magnetic field in the envelope region ($\theta_{\rm B}^{\rm env}$). In both clouds, the magnetic fields are found to be aligned with the galactic field (θGP). Due to their close proximity to the galactic plane, we incorporate the values of offset ($\theta _{\rm off} = |\theta _{\rm B}^{\rm env} - \theta _{\rm GP}|$) from this study into our previously established geometrical correlation between the variation in relative orientation with the galactic longitude. We find that the offsets estimated for two clouds L1495 and L1498 in this investigation are consistent with the offsets predicted by our previous study. We also generate the extinction map for the two clouds using the NICE technique, and the extinction structure is found to be analogous to the dust structure at the denser region of the cloud as obtained from the 500 µm dust continuum emission maps of Herschel1 SPIRE. Three distinct cores with high extinction are detected in L1495, and two such cores are also detected in L1498. We also estimate the mean particle density ($\lt n_{\rm H_2}\gt $) at the peripheral region as well as the high extinction cores of the clouds.
Doppler signature of a possible termination shock in an off-limb solar flare
Authors: French Ryan J , Yu Sijie , Chen Bin , Shen Chengcai , Matthews Sarah A 
Published: February 2024

ABSTRACT
We report striking Doppler velocity gradients observed during the well-observed 2017 September 10 solar flare, and argue that they are consistent with the presence of an above-the-looptop termination shock beneath the flare current sheet. Observations from the Hinode Extreme-ultraviolet Imaging Spectrometer measure plasma sheet Doppler shifts up to 35 km s−1 during the late-phase of the event. By comparing these line-of-sight flows with plane-of-sky (POS) measurements, we calculate total velocity downflows of 200+ km s−1, orientated ≈6–10° out of the POS. The observed velocities drop rapidly at the base of the hot plasma sheet seen in extreme ultraviolet, consistent with simulated velocity profiles predicted by our 2.5D magnetohydrodynamics model that features a termination shock at the same location. Finally, the striking velocity deceleration aligns spatially with the suppression of Fe xxiv non-thermal velocities, and a 35–50 keV hard X-ray looptop source observed by the Reuven Ramaty High Energy Solar Spectroscopic Imager. Together, these observations are consistent with the presence of a possible termination shock within the X8.2-class solar flare.
Calcium complexation by steroids involved in the steroidogenesis
Authors: Siless Gastón E , Cabrera Gabriela M 
Published: February 2024

Abstract
Steroids that take part in the pathways of human steroidogenesis are involved in many biological mechanisms where they interact with calcium. In the present work, the binding selectivities and affinities for calcium of progestagens, mineralocorticoids, androstagens, and estrogens were studied by Electrospray Ionization-Mass Spectrometry (ESI-MS). The adduct profile of each steroid was characterized by high resolution and tandem mass spectrometry. The relative stability of the most important adducts was studied by threshold collision induced dissociation, E1/2. Doubly-charged steroid-calcium complexes [nM + Ca]2+ with n = 1–6 were predominant in the mass spectra. The adduct [5M + Ca]2+ was the base peak for most 3-keto-steroids, while ligands bearing hindered ketones or α-hydroxy-ketones also yielded [nM + Ca + mH2O]2+ with n = 3–4 and m = 0–1. Principal component analysis allowed us to spot the main differences and similarities in the binding behavior of these steroids. The isomers testosterone and dehydroepiandrosterone, androstanolone and epiandrosterone, and 17-α-hydroxyprogesterone and 11-deoxycorticosterone showed remarkable differences in their adduct profiles. Computational modeling of representative adducts was performed by density functional theory methods. The possible binding modes at low and high numbers of steroid ligands were determined by calcium Gas Phase Affinity, and through modeling of the complexes and comparison of their relative stabilities, in agreement with the experimental results.
An evolutionarily conserved phosphoserine-arginine salt bridge in the interface between ribosomal proteins uS4 and uS5 regulates translational accuracy in Saccharomyces cerevisiae
Authors: Joshi Kartikeya , Luisi Brooke , Wunderlin Grant , Saleh Sima , Lilly Anna , Okusolubo Temiloluwa , Farabaugh Philip J 
Published: February 2024

Abstract
Protein-protein and protein–rRNA interactions at the interface between ribosomal proteins uS4 and uS5 are thought to maintain the accuracy of protein synthesis by increasing selection of cognate aminoacyl-tRNAs. Selection involves a major conformational change—domain closure—that stabilizes aminoacyl-tRNA in the ribosomal acceptor (A) site. This has been thought a constitutive function of the ribosome ensuring consistent accuracy. Recently, the Saccharomyces cerevisiae Ctk1 cyclin-dependent kinase was demonstrated to ensure translational accuracy and Ser238 of uS5 proposed as its target. Surprisingly, Ser238 is outside the uS4-uS5 interface and no obvious mechanism has been proposed to explain its role. We show that the true target of Ctk1 regulation is another uS5 residue, Ser176, which lies in the interface opposite to Arg57 of uS4. Based on site specific mutagenesis, we propose that phospho-Ser176 forms a salt bridge with Arg57, which should increase selectivity by strengthening the interface. Genetic data show that Ctk1 regulates accuracy indirectly; the data suggest that the kinase Ypk2 directly phosphorylates Ser176. A second kinase pathway involving TORC1 and Pkc1 can inhibit this effect. The level of accuracy appears to depend on competitive action of these two pathways to regulate the level of Ser176 phosphorylation.
Nucleotide-level distance metrics to quantify alternative splicing implemented in TranD
Authors: Nanni Adalena , Titus-McQuillan James , Bankole Kinfeosioluwa S , Pardo-Palacios Francisco , Signor Sarah , Vlaho Srna , Moskalenko Oleksandr , Morse Alison M , Rogers Rebekah L , Conesa Ana , McIntyre Lauren M 
Published: February 2024

Abstract
Advances in affordable transcriptome sequencing combined with better exon and gene prediction has motivated many to compare transcription across the tree of life. We develop a mathematical framework to calculate complexity and compare transcript models. Structural features, i.e. intron retention (IR), donor/acceptor site variation, alternative exon cassettes, alternative 5′/3′ UTRs, are compared and the distance between transcript models is calculated with nucleotide level precision. All metrics are implemented in a PyPi package, TranD and output can be used to summarize splicing patterns for a transcriptome (1GTF) and between transcriptomes (2GTF). TranD output enables quantitative comparisons between: annotations augmented by empirical RNA-seq data and the original transcript models; transcript model prediction tools for longread RNA-seq (e.g. FLAIR versus Isoseq3); alternate annotations for a species (e.g. RefSeq vs Ensembl); and between closely related species. In C. elegans, Z. mays, D. melanogaster, D. simulans and H. sapiens, alternative exons were observed more frequently in combination with an alternative donor/acceptor than alone. Transcript models in RefSeq and Ensembl are linked and both have unique transcript models with empirical support. D. melanogaster and D. simulans, share many transcript models and long-read RNAseq data suggests that both species are under-annotated. We recommend combined references.
RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination
Authors: Joo Jeong H , Hong Soogil , Higashide Mika T , Choi Eui-Hwan , Yoon Seobin , Lee Min-Su , Kang Hyun Ah , Shinohara Akira , Kleckner Nancy , Kim Keun P 
Published: February 2024

Abstract
Meiotic recombination is initiated by programmed double-strand breaks (DSBs). Studies in Saccharomyces cerevisiae have shown that, following rapid resection to generate 3′ single-stranded DNA (ssDNA) tails, one DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas the other end remains associated with its sister. Then, after regulated differentiation into crossover- and noncrossover-fated types, the second DSB end participates in the reaction by strand annealing with the extended first end, along both pathways. This second-end capture is dependent on Rad52, presumably via its known capacity to anneal two ssDNAs. Here, using physical analysis of DNA recombination, we demonstrate that this process is dependent on direct interaction of Rad52 with the ssDNA binding protein, replication protein A (RPA). Furthermore, the absence of this Rad52-RPA joint activity results in a cytologically-prominent RPA spike, which emerges from the homolog axes at sites of crossovers during the pachytene stage of the meiotic prophase. Our findings suggest that this spike represents the DSB end of a broken chromatid caused by either the displaced leading DSB end or the second DSB end, which has been unable to engage with the partner homolog-associated ssDNA. These and other results imply a close correspondence between Rad52-RPA roles in meiotic recombination and mitotic DSB repair.
Unraveling the complex relationship between mRNA and protein abundances: a machine learning-based approach for imputing protein levels from RNA-seq data
Authors: Prabahar Archana , Zamora Ruben , Barclay Derek , Yin Jinling , Ramamoorthy Mahesh , Bagheri Atefeh , Johnson Scott A , Badylak Stephen , Vodovotz Yoram , Jiang Peng 
Published: February 2024

Abstract
The correlation between messenger RNA (mRNA) and protein abundances has long been debated. RNA sequencing (RNA-seq), a high-throughput, commonly used method for analyzing transcriptional dynamics, leaves questions about whether we can translate RNA-seq-identified gene signatures directly to protein changes. In this study, we utilized a set of 17 widely assessed immune and wound healing mediators in the context of canine volumetric muscle loss to investigate the correlation of mRNA and protein abundances. Our data reveal an overall agreement between mRNA and protein levels on these 17 mediators when examining samples from the same experimental condition (e.g. the same biopsy). However, we observed a lack of correlation between mRNA and protein levels for individual genes under different conditions, underscoring the challenges in converting transcriptional changes into protein changes. To address this discrepancy, we developed a machine learning model to predict protein abundances from RNA-seq data, achieving high accuracy. Our approach also effectively corrected multiple extreme outliers measured by antibody-based protein assays. Additionally, this model has the potential to detect post-translational modification events, as shown by accurately estimating activated transforming growth factor β1 levels. This study presents a promising approach for converting RNA-seq data into protein abundance and its biological significance.
Transcription factor PagMYB31 positively regulates cambium activity and negatively regulates xylem development in poplar
Authors: Zhang Yanhui , Chen Song , Xu Linghua , Chu Shimin , Yan Xiaojing , Lin Lanying , Wen Jialong , Zheng Bo , Chen Su , Li Quanzi 
Published: February 2024

Abstract
Wood formation involves consecutive developmental steps, including cell division of vascular cambium, xylem cell expansion, secondary cell wall (SCW) deposition, and programmed cell death. In this study, we identified PagMYB31 as a coordinator regulating these processes in Populus alba × Populus glandulosa and built a PagMYB31-mediated transcriptional regulatory network. PagMYB31 mutation caused fewer layers of cambial cells, larger fusiform initials, ray initials, vessels, fiber and ray cells, and enhanced xylem cell SCW thickening, showing that PagMYB31 positively regulates cambial cell proliferation and negatively regulates xylem cell expansion and SCW biosynthesis. PagMYB31 repressed xylem cell expansion and SCW thickening through directly inhibiting wall-modifying enzyme genes and the transcription factor genes that activate the whole SCW biosynthetic program, respectively. In cambium, PagMYB31 could promote cambial activity through TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF)/PHLOEM INTERCALATED WITH XYLEM (PXY) signaling by directly regulating CLAVATA3/ESR-RELATED (CLE) genes, and it could also directly activate WUSCHEL HOMEOBOX RELATED4 (PagWOX4), forming a feedforward regulation. We also observed that PagMYB31 could either promote cell proliferation through the MYB31-MYB72-WOX4 module or inhibit cambial activity through the MYB31-MYB72-VASCULAR CAMBIUM-RELATED MADS2 (VCM2)/PIN-FORMED5 (PIN5) modules, suggesting its role in maintaining the homeostasis of vascular cambium. PagMYB31 could be a potential target to manipulate different developmental stages of wood formation.
Deterrence effects despite lack of prosecution: Punishment outcomes of white-collar crime investigations in Norway
Authors: Gottschalk Petter 
Published: February 2024

Abstract
The national authority Økokrim was established several decades ago to become a policing centre of excellence in the investigation and prosecution of economic and environmental crime in Norway that is especially concerned with white-collar and corporate crime. This article focusses on deterrence effects from Økokrim investigations that never lead to prosecution or conviction. The research raises the issue of the use of law enforcement publicity regarding investigations of potential offenders, often long before possible convictions. The issue is explored by the press releases from the Norwegian body. The apparent shift in strategy and practice at Økokrim might be explained by the devastating evaluation of the national authority some years ago. The evaluation was initiated after the Transocean court scandal where all defendants were acquitted of all charges. Deterrence strategy by investigations implies that Økokrim passes negative consequences on suspects who never have a chance to defend themselves in court. In jurisdictions with criminal justice, a suspect should always have the benefit of the doubt. Conviction should only occur when guilt is proven beyond any reasonable and sensible doubt. Until eventually proven guilty, a suspect is supposed to be considered innocent. This is a basic principle of justice.
Police perception of their assault victimization risk and advice to cope with the risk
Authors: Yanich Daniel B , Gibbs Jennifer C 
Published: February 2024

Abstract
Policing can be a dangerous profession. While the rate of intentional violence against the police has declined slightly over the past few decades, police rhetoric seems to communicate the opposite. Given this messaging, do officers accurately assess their own risk of violence? To address this question, interviews were conducted with 184 police officers in Pennsylvania. Participants were asked about (1) the risk of assault on police officers compared to 5 years ago; (2) the risk of assault on officers in their own department compared to other police departments; and (3) how they would advise others to cope with this risk. Bivariate analyses indicate that respondents thought police are more likely to be assaulted now compared to 5 years ago. Other findings and implications of these results will be discussed, along with officer advice on how to cope with the risk of policing.
Advocacy coalitions as political organizations
Authors: Nohrstedt Daniel , Heinmiller Tim 
Published: February 2024

Abstract
Conceptually, advocacy coalitions are referenced in several policy process theories and frameworks to describe groups of actors that share beliefs and coordinate efforts to influence public policy. In the past decades, advocacy coalitions have received increased attention as a concept and a theoretical approach to understanding collective action in the policy process. In this study, we argue that despite its growing popularity, past empirical research has mainly focused on identifying and describing advocacy coalitions while largely overlooking their role and impact as political organizations. Many of the core premises and assumptions about advocacy coalitions hereby remain understudied and untested. Here, we depart from the Advocacy Coalition Framework (ACF) to discuss the political organization of advocacy coalitions by focusing on four dimensions: (1) a basis for engagement in joint strategies, (2) capacity to mobilize political resources, (3) ability to gain influence in policy processes, and (4) perceptions of advocacy coalitions as a political entity. We briefly review the theory and evidence of each dimension and conclude that several core assumptions about advocacy coalitions yet remain to be empirically tested to enable further conceptual specification and theory development within the ACF and beyond. To this end, we propose a research agenda with suggested research questions, designs, and methodological considerations for advancing empirical research on the role and impact of advocacy coalitions in different cases and contexts.