bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for perioperative blood transfusion in patients undergoing total laparoscopic hysterectomy
Authors: Cao Xianghua , Liu Xueliang , Zhang Xingxing , Zhang Kefang , Chen Chuan , Yang Qinfeng , Wang Jian , Li Xueping , Wei Ling 
Published: January 2024

Abstract
Purpose
The goal is to identify risk factors associated with receiving a blood transfusion during the perioperative period in patients who undergo total laparoscopic hysterectomy (TLH) using a large-scale national database.

Methods
In this retrospective analysis, data from the Nationwide Inpatient Sample (NIS) was utilized to review the medical records of all patients who underwent TLH from 2010 to 2019. The researchers identified patients who had received a blood transfusion during the perioperative period and compared with those who had not. The subsequent factors associated with blood transfusion were examined: hospital characteristics (type of admission and payer, patient demographics (age and race), bed size, teaching status, location, and region of hospital), length of stay (LOS), total charges during hospitalization, in-hospital mortality, comorbidities, and perioperative complications. The data was analyzed using descriptive statistics. The independent risk factors of perioperative blood transfusion after TLH was identified by performing multivariate logistic regression.

Results
A total of 79,933 TLH were captured from the NIS database, among which 3433 (4.40%) patients received a perioperative blood transfusion. TLH patients affected by blood transfusion were 2 days longer hospital stays (P < 0.001), higher overall costs (P < 0.001), the patients who received a transfusion after a long-term hospitalization had a significantly higher rate of mortality (0.5% vs. 0.1%; P < 0.001). Perioperative blood transfusion after TLH was associated with chronic blood loss anemia, deficiency anemia, coagulopathy, congestive heart failure, fluid and electrolyte disorders, renal failure, metastatic cancer, sepsis, weight loss, deep vein thrombosis, gastrointestinal hemorrhage, shock, acute myocardial infarction, and pneumonia, stroke, hemorrhage, pulmonary embolism, and disease of the genitourinary system.

Conclusion
Studying the risk factors of perioperative blood transfusion after TLH is advantageous in order to ensure proper management and optimize outcomes.
Midregional proatrial naturetic peptide (MRproANP) and copeptin (COPAVP) as predictors of all-cause mortality in recently diagnosed mild to moderate COPD—results from COSYCONET
Authors: Fähndrich S. , Herr C. , Teuteberg S. , Alter P. , Söhler S. , Soriano D. , Classen J. , Adams J. , Weinhold V. , Watz H. , Waschki B. , Zeller T. , Eichenlaub M. , Trudzinski F. C. , Michels J. D. , Omlor A. , Seiler F. , Moneke I. , Biertz F. , Stolz D. , Welte T. , Kauczor H. U. , Kahnert K. , Jörres R. A. , Vogelmeier C. F. , Bals R. ,   the German COSYCONET Cohort, Andreas Stefan , Alter Peter , Bals Robert , Behr Jürgen , Kahnert Kathrin , Bahmer Thomas , Bewig Burkhard , Ewert Ralf , Stubbe Beate , Ficker Joachim H , Grohé Christian , Held Matthias , Henke Markus , Herth Felix , Kirsten Anne-Marie , Watz Henrik , Koczulla Rembert , Kronsbein Juliane , Kropf-Sanchen Cornelia , Herzmann Christian , Pfeifer Michael , Randerath Winfried J , Seeger Werner , Studnicka Michael , Taube Christian , Timmermann Hartmut , Schmeck Bernd , Vogelmeier Claus , Welte Tobias , Wirtz Hubert 
Published: January 2024

Abstract
Background
MRproANP and COPAVP are prognostic markers for mortality in chronic obstructive pulmonary disease (COPD). Furthermore, these biomarkers predict mortality due to cardiovascular diseases, which are important prognostically determining comorbidities in patients with COPD. However, less is known about these biomarkers in recently diagnosed mild to moderate COPD. Therefore, we analyzed these biomarkers as potential predictors of mortality in recently diagnosed mild to moderate COPD.

Methods
The blood biomarkers considered were copeptin (COPAVP), midregional adrenomedullin (MRproADM), midregional proatrial naturetic peptide (MRproANP), and fibrinogen. Analyses were performed in patients with stable “recently diagnosed mild to moderate COPD” defined by GOLD grades 0–2 and diagnosis of COPD ≤ 5 years prior to inclusion into the COSYCONET cohort (COPD and Systemic Consequences—Comorbidities Network), using Cox regression analysis with stepwise adjustment for multiple COPD characteristics, comorbidities, troponin and NT-proBNP.

Results
655 patients with recently diagnosed mild to moderate COPD were included. In the initial regression model, 43 of 655 patients died during the 6-year follow-up, in the final model 27 of 487. Regression analyses with adjustment for confounders identified COPAVP and MRproANP as statistically robust biomarkers (p < 0.05 each) of all-cause mortality, while MRproADM and fibrinogen were not. The fourth quartile of MRproANP (97 pmol/L) was associated with a hazard ratio of 4.5 (95%CI: 1.6; 12.8), and the fourth quartile of COPAVP (9.2 pmol/L) with 3.0 (1.1; 8.0). The results for MRproANP were confirmed in the total cohort of grade 0–4 (n = 1470 finally).

Conclusion
In patients with recently diagnosed mild to moderate COPD, elevated values of COPVP and in particular MRproANP were robust, independent biomarkers for all-cause mortality risk after adjustment for multiple other factors. This suggests that these markers might be considered in the risk assessment of early COPD.
ChOP-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomography
Authors: Parobková Viktória , Kompaníková Petra , Lázňovský Jakub , Kavková Michaela , Hampl Marek , Buchtová Marcela , Zikmund Tomáš , Kaiser Jozef , Bryja Vítězslav 
Published: January 2024

AbstractThe Hindbrain Choroid Plexus is a complex, cerebrospinal fluid-secreting tissue that projects into the 4th vertebrate brain ventricle. Despite its irreplaceability in the development and homeostasis of the entire central nervous system, the research of Hindbrain Choroid Plexus and other Choroid Plexuses has been neglected by neuroscientists for decades. One of the obstacles is the lack of tools that describe the complex shape of the Hindbrain Choroid Plexus in the context of brain ventricles. Here we introduce an effective tool, termed ChOP-CT, for the noninvasive, X-ray micro-computed tomography-based, three-dimensional visualization and subsequent quantitative spatial morphological analysis of developing mouse Hindbrain Choroid Plexus. ChOP-CT can reliably quantify Hindbrain Choroid Plexus volume, surface area, length, outgrowth angle, the proportion of the ventricular space occupied, asymmetries and general shape alterations in mouse embryos from embryonic day 13.5 onwards. We provide evidence that ChOP-CT is suitable for the unbiased evaluation and detection of the Hindbrain Choroid Plexus alterations within various mutant embryos. We believe, that thanks to its versatility, quantitative nature and the possibility of automation, ChOP-CT will facilitate the analysis of the Hindbrain Choroid Plexus in the mouse models. This will ultimately accelerate the screening of the candidate genes and mechanisms involved in the onset of various Hindbrain Choroid Plexus-related diseases.
The impact of two radical sternectomy surgical techniques on the outcome of deep sternal wound infections
Authors: Bota Olimpiu , Taqatqeh Feras , Bönke Florian , Mülhausen Maxime , Matschke Klaus , Dragu Adrian , Bienger Kevin , Rasche Stefan 
Published: January 2024

Abstract
Background
Deep sternal wound infection (DSWI) is a rare, yet devastating complication after cardiac surgery. While the surgical treatment always implies the soft tissue and bone debridement, there is little data about this procedure. The aim of our study was to evaluate the impact of the radical sternectomy on the outcome in patients with DSWI and to identify the risk factors which could influence the result. The surgical techniques of piecemeal sternectomy and the newly developed en bloc sternectomy were also evaluated.

Methods
The study was developed as a retrospective cohort study. 86 patients with DSWI who received a radical sternal resection at our institution between March 2018 and December 2021 were included.

Results
The average age of the cohort was 67.3 ± 7.4 years, and 23.3% of patients were female. The average length of stay trended shorter after en bloc sternectomy (median 26 days) compared to piecemeal sternectomy (37 days). There were no significant differences between the piecemeal and en bloc sternal resection techniques. Anticoagulant and antiplatelet drugs had no significant influence on bleeding and transfusion rates. Obese patients showed an increased risk for postoperative bleeding requiring reintervention. Transfusion of packed red blood cells was significantly associated with lower hemoglobin values before surgery and ASA Class 4 compared to ASA Class 3. The in-hospital mortality was 9.3%, with female sex and reintervention for bleeding as significant risk factors. Nine patients developed an infection relapse as a chronic fistula at the level of clavicula or ribs, with ASA Class 4 as a risk factor.

Conclusion
Radical sternectomy is a safe procedure to treat DSWI with compromised sternal bone. Both piecemeal and en bloc techniques ensure reliable results, while complications and mortality appear to be patient-related.
Mir-338-3p targeting THBS1 attenuates glioma progression by inhibiting the PI3K/Akt pathway
Authors: Jiang Lianglei , Fang Ting , Hu Tingting , Feng Jun , Yan Pengfei 
Published: January 2024

Abstract
Background
Glioma is a brain tumor with high morbidity and mortality rates. Understanding its molecular pathogenesis can provide targets and therapeutic strategies for glioma treatment. miR-338-3p represses tumor growth in several cancers, including glioma. Thus, this study aimed to identify the regulatory effects of miR-338-3p/phosphoinositide 3-kinase (PI3K)/Akt/thrombospondins 1 (THBS1) on glioma progression.

Materials and methods
Quantitative reverse transcription polymerase chain reaction and western blotting were performed to evaluate the levels of miR-338-3p, THBS1, and PI3K/Akt phosphorylation-related proteins. TargetScan software predicted that miR-338-3p targeted THBS1. This was confirmed by performing the dual-luciferase assay. Wound-healing and cell-counting-kit-8 experiments were performed to analyze how THBS1 and miR-338-3p affect the ability of glioma cells to migrate and proliferate. The effect of miR-338-3p on tumorigenicity in mice was also analyzed.

Results
miR-338-3p downregulation was observed in gliomas, whereas THBS1 showed the opposite trend. By suppressing the PI3K/Akt signaling pathway activation, miR-338-3p overregulated the ability of glioma cells to migrate and proliferate in vitro. Additionally, miR-338-3p inhibited the development of glioma tumors in vivo. Moreover, miR-338-3p directly targeted THBS1. THBS1 overexpression promoted glioma cell migration and proliferation by increasing PI3K/Akt phosphorylation. Nonetheless, miR-338-3p overregulation alleviated the effects of THBS1 overexpression.

Conclusion
The miR-338-3p/PI3K/Akt/THBS1 regulatory axis can modulate the progression of glioma cell proliferation and migration; thus, it can be considered a therapeutic biomarker.
Temporal phytoplankton dynamics and environmental variables in four Ethiopian soda lakes
Authors: Melese Hana , Debella Habte Jebessa 
Published: January 2024

AbstractWe investigated the spatio-temporal dynamics of phytoplankton composition, chlorophyll-a as a proxy for algal biomass, and abundance in relation to environmental parameters in four Ethiopian soda lakes: Arenguade, Beseka, Chittu, and Shala. Triplicate water samples were collected from each lake from January to December 2020, four times in different seasons. Lake Chittu had the highest chlorophyll-a concentration, followed by Lake Arenguade, Beseka and Shala. Chlorophyll-a concentrations generally increased during the post rainy and dry season. The results of LR models are high for lakes Arengude, Beseka and Chittu. Lakes Shala and Beseka had the highest number of phytoplankton taxa, with both taxa composition and abundance dominated by Bacillariophyceae. Cyanoprokaryota, particularly Limnospira fusiformis, predominated in the abundance of Lakes Arenguade and Chittu. Water temperature, Secchi depth, turbidity, electrical conductivity, soluble reactive phosphorus, nitrate and silica significantly influenced the phytoplankton community structure. Long-term trend analysis revealed changes in phytoplankton biomass and lake taxonomic composition. The alteration in phytoplankton biomass and species composition of the lakes could be attributed to three factors: (1) frequent high-velocity explosions conducted for seismological studies in the past. This impact caused a dramatic increase in lake level in the case of Lake Beseka leading to a drop in nutrient concentration; (2) climate change and (3) salt content. Overall, our findings suggest that phytoplankton composition, biomass, and abundance varied according to seasonal fluctuations, emphasizing the possible effects of anthropogenic and natural causes on their community structure.
Role of Interleukin-17 family cytokines in disease severity of patients with knee osteoarthritis
Authors: Kamiab Zahra , Khorramdelazad Hossein , Kafi Mehdi , Jafarzadeh Abdollah , Mohammadi-Shahrokhi Vahid , Bagheri-Hosseinabadi Zahra , Saeed Askari Pooya , Abbasifard Mitra 
Published: January 2024

Abstract
Background
Interleukin-17 (IL-17) family plays a role in the pathogenesis of knee osteoarthritis (KOA) by contributing to the inflammatory and destructive processes in the affected joint. This study aimed to measure levels of IL-17 A and IL-25 (IL-17E) in serum of KOA patients and determine their roles in the disease severity of patients.

Methods
In this, 34 patients with KOA and 30 age and sex-matched healthy subjects (HS) were enrolled. Patients were categorized based on their Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Visual Analog Scale (VAS), and Body Mass Index (BMI) scores. The enzyme-linked immunosorbent assay (ELISA) technique was employed to measure serum levels of IL-17 A and IL-25.

Results
Level of IL-25 was significantly higher (P < 0.0001) in the KOA subjects than HS. IL-17 A level was significantly higher in KOA cases with WOMAC < 40 (P < 0.0001) in comparison to HS. IL-25 level was significantly higher in the KOA cases with WOMAC < 40 (P < 0.0001) and with WOMAC ≥ 40 (P < 0.0001) compared to HS. IL-17 A concentration was significantly higher in the KOA cases with VAS < 5 (P < 0.0001) compared to HS. IL-25 level was significantly higher in the KOA cases with VAS < 5 (P < 0.0001) and with VAS ≥ 5 (P < 0.0001) in comparison to HS. KOA patients with BMI ≥ 30 had significantly higher IL-17 A and IL-25 concentration in comparison to HS.

Conclusions
The serum level of IL-25 in KOA patients is increased probably due to negative controlling feedback on inflammatory responses, which can be associated with obesity and disease activity.
Effective treatment of NR2F1-related epilepsy with perampanel
Authors: Li Xiao , Gao Kai , Li Yutang , Zhang Yuehua , Zhang Han , Jiang Yuwu 
Published: January 2024

Abstract
Background
NR2F1 mutations are associated with Bosch-Boonstra-Schaaf optic atrophy syndrome (BBSOAS). Although ~ 46.7% of BBSOAS patients present with epilepsy, which is always drug-resistant and associated with higher rates of behavioral and cognitive problems, the treatment and outcomes of NR2F1-related epilepsy have rarely been described. Here, we present new cases of BBSOAS-related epilepsy and summarize all previously reported cases to explore the effective treatment for this type of epilepsy.

Methods
We identified six new Chinese cases of BBSOAS with epilepsy. Five different de novo heterozygous NR2F1 mutations were identified in these cases, including two novel mutations c.365G > T, p.Cys122Phe and c.449G > T, p.Gly150Val. By combining the six cases and 14 previously reported cases, we analyzed the characteristics and treatment outcomes of NR2F1-related epilepsy.

Results
Twelve of the 20 patients (60%) had infantile epileptic spasms, while the other patients had generalized tonic/tonic-clonic, focal, myoclonic, absence, or unclassified seizures. Several anti-seizure medications, steroids, and a ketogenic diet were administered in these cases. However, seizures were controlled in only 50% of previously reported cases, while all of the six new cases became seizure-free after perampanel as an add-on treatment. The average time from the addition of perampanel to seizure control was 7.33 ± 4.59 months (range, 1–12 months). The median time to seizure freedom was 14 months (1–32 months, > 19 months in 3 cases). The average dosage of perampanel needed for epilepsy control was 0.22 ± 0.17 mg/kg per day.

Conclusions
In this paper, we comprehensively summarized the clinical characteristics, treatments and outcomes of NR2F1-related epilepsy for the first time. Perampanel exhibits dramatic efficacy for NR2F1-related epilepsy. This will help optimize the treatment of this type of epilepsy and provide clues for its pathogenic mechanisms. The two novel mutations expand the genotype spectrum of this disease.
Efficacy of lumbar motor control training in treatment of patients with cervicogenic headache
Authors: Marzok Hagar A. , Ashry Ahmed H. , Sedhom Magda G. , Abd El-Raoof Neveen A. 
Published: January 2024

Abstract
Background
Cervicogenic headache (CGH), is a secondary headache arising from cervical disorders. Training core muscles have a corrective effect on the whole spine. Moreover, increased deep neck flexors (DNFs) activation is closely linked with balanced core stabilization of the global cervical-thoracic-lumbopelvic chains. This study aimed to investigate the effect of lumbar motor control training combined with cervical stabilization exercises compared to cervical stabilization exercises alone in CGH patients.

Methods
Fifty-two subjects of both genders with CGH and chronic mechanical neck pain (CMNP) participated in this study. They were recruited from the outpatient Physical Therapy Clinic at Mubarak Central Hospital, Giza, Egypt; their mean age was 28.1 ± 5.8 years and their BMI was 22.8 ± 1.6 kg/m2. They received treatment for three sessions per week for 4 weeks. Subjects were assigned randomly into two equal groups; the control group: received cervical stabilization exercises (axial elongation, cranio-cervical flexion, cervical extension, rowing, and Y-exercise). Study group: received cervical stabilization exercise in addition to lumbar motor control training (abdominal draw-in maneuver, side plank, and quadruped position), each exercise had a 4-week progression. Headache frequency, duration, intensity by visual analog scale (VAS), and neck functional disability by Neck Disability Index (NDI) were measured before the first session and after the last session of the 4 weeks of treatment.

Results
There was a high statistically significant difference in post-treatment mean values of headache frequency, headache duration, headache intensity, and neck functional disability between the two groups in favor of the study group.

Conclusion
Adding lumbar motor control training to cervical stabilization exercise is more effective than cervical stabilization alone in decreasing headache frequency, duration, intensity, and neck functional disability in CGH patients with CMNP.

Trial registration
NCT05952115. Registered 11 July 2023-retrospectively registered, https://register.clinicaltrials.gov/prs/app/action/LoginUser?ts=1&cx=-jg9qo4.
The use of Arabic Language Sample Analysis as a screening tool in kindergarten Egyptian bilingual children
Authors: Osman Dalia Mostafa , Abdel Hamid Asmaa Ahmed , Maged Samar , Abdel Hady Aisha Fawzy 
Published: January 2024

Abstract
Background
Language sample analysis is a naturalistic method used for measuring child’s expressive language abilities. It is used in case of difficulty in direct interaction in remote places or pandemics.

Aim of the work
The aim of this study is to evaluate the use of a language sample analysis as an easy applicable screening tool for narrative ability in kindergarten Egyptian bilingual children in order to reach a better understanding of their language profile. Subjects and methods: 150 bilingual Egyptian preschool male and female children exposed to Arabic and English languages were included and divided into two groups according to their age. All were subjected to the BEA-LSA narration assessment including macrostructure, microstructure items, and conversation assessment including verbal and nonverbal items.

Results
The results revealed that bilingual children showed better performance in English than Arabic in most of the narration and conversation tasks. However, there was equal development between both age groups in English language regarding temporality, connectives, and reference clarity. The older age group showed higher code switching than younger age group and code switching occurred more from Arabic to English language. Both age groups bilingual children showed better frequency of words and mean length of utterance in narration. While in conversation, both age groups showed better type token ratio. Language sample analysis showed good test–retest reliability.

Conclusion
Language sample analysis could identify the characteristic profile of bilingual kindergarten Egyptian children and was a reliable method to analyze both narration and conversation skills of bilingual children.
Advances in Staging of schizophrenia. Development of an empirical staging model for schizophrenia.
Authors: Garcia-Portilla P. 
Published: January 2024

Abstract
After a short review of the state of the art of clinical staging in schizophrenia, I will present a recently developed empirical staging model.

Methods
Two hundred twelve stable outpatients with schizophrenia from Oviedo (Spain) were assessed with: an ad hoc questionnaire (demographic, clinical information); psychopathology: PANSS, CDS, OSQ, CGI-S; functioning: PSP; cognition: MATRICS; lab tests: C-Reactive Protein, IL-1RA, IL-6, Platelet/Lymphocyte (PLR), Neutrophil/Lymphocyte (NLR), and Monocyte/Lymphocyte (MLR) ratios.
An ad hoc genetic algorithm (GA) was developed to select those variables showing the best performance for patients’ CGI classification. The objective function of the GA maximizes the individual’s correct classification of a support vector machines (SVM) model that employs as input variables those given by the GA. Models’ performance was assessed with the help of 3-fold cross-validation, and this process was repeated 10,000 times for each one of the models evaluated. Once developed, we used the ANOVA test (Duncan’s post-hoc) for all the variables included in the model to demonstrate its construct validity.

Results
Our model included the following variables: positive, negative, depressive, and general psychopathological symptoms, processing speed, visual learning, social cognition, and real-world functioning. Its classification accuracy is 64.54% (SD=4.83%) with a specificity and sensitivity of 0.85 and 0.63. The external validity of the new model is being tested using a French sample from the FACE-SZ (FondaMental Advanced Centers of Expertise-Schizophrenia) cohort.

Conclusions
We developed an SVM model including psychopathological, cognitive, and functional variables.

Our model demonstrated good construct validity since all the variables included behaved as expected. That is, they score significantly worse as the patients’ severity increases. Besides, it showed good accuracy, specificity, and sensitivity classification properties.
Using staging models in daily clinical practice will help clinicians to better.

Disclosure of Interest
None Declared