bluuebunny/crossref_metadata_2025_split huggingface dataset:
In patients with myelodysplastic syndromes response to rHuEPO and G‐CSF treatment is related to an increase of cytogenetically normal CD34+ cells
Authors: Rigolin Gian Matteo , Porta Matteo Della , Ciccone Maria , Bugli Anna Maria , Bragotti Letizia Zenone , Mauro Endri , Fraulini Chiara , Rossi Antonella Russo , Bardi Antonella , Cuneo Antonio , Castoldi Gianluigi 
Published: July 2004

SummaryThe in vivo effect of recombinant human erythropoietin (rHuEpo) and granulocyte colony‐stimulating factor (G‐CSF) combined treatment on CD34+ cells was evaluated by fluorescence in situ hybridization (FISH) in 13 myelodysplastic syndrome (MDS) patients with known cytogenetic abnormalities. After treatment, responsive patients presented a significantly lower proportion of FISH abnormal CD34+ cells than before treatment (P = 0·003), and in comparison with unresponsive cases (P = 0·007). Response to treatment was associated with a reduced degree of apoptosis in CD34+ cells (P = 0·021): however, no difference in telomere length was observed in responsive patients after growth factor administration. Although the number of patients analysed was relatively small, the present data suggest that, in MDS patients, response to rHuEpo and G‐CSF may be related to the proliferation of karyotypically normal but potentially defective CD34+ progenitor cells.
Dendritic cells fused with core binding factor‐beta positive acute myeloid leukaemia blast cells induce activation of cytotoxic lymphocytes
Authors: Banat G.‐Andre , Usluoglu Nurguel , Hoeck Marianne , Ihlow Kerstin , Hoppmann Sabine , Pralle Hans 
Published: July 2004

SummarySeveral reports have described various strategies of dendritic cell (DC) vaccination to induce specific T‐cell responses in patients with acute myeloid leukaemia (AML). About 50–60% of AML cases blasts have chromosomal abnormalities, such as inv(16) or t(8,21), which could encode for leukaemia‐specific antigenic peptide sequences, possibly presented in the context of self‐major histocompatibility complex (MHC) molecules. As the co‐culture of AML blasts with T lymphocytes seldom resulted in T‐cell stimulation, we fused AML blasts with autologous DC to enhance this effect. The fusion cells expressed MHC class I and II, CD40, B7‐1, B7‐2, CD209 and several adhesion molecules. In a mixed lymphocyte hybrid reaction, the fusion cells induced the proliferation of autologous T cells. Moreover, in the special case of fusion cells established from AML blasts with the chromosomal abnormality inv(16), the autologous T lymphocytes could be primed to induce cytotoxicity against up to 70% autologous AML blasts in a effector:target ratio of 20:1. Blocking assays demonstrated that the lysis was chiefly mediated by CD8+, CCR7− T lymphocytes, which could be further expanded in the form of effector memory CD8+ T cells by repeated co‐cultures with the autologous fusion cells.
Effects of genetic fusion of factor IX to albumin on in vivo clearance in mice and rabbits
Authors: Sheffield William P. , Mamdani Asif , Hortelano Gonzalo , Gataiance Sharon , Eltringham‐Smith Louise , Begbie Megan E. , Leyva Rina A. , Liaw Peter S. , Ofosu Frederick A. 
Published: July 2004

SummaryIndividuals with haemophilia B require replacement therapy with recombinant or plasma‐derived coagulation factor IX (fIX). More benefit per injected dose might be obtained if fIX clearance could be slowed. The contribution of overall size to fIX clearance was explored, using genetic fusion to albumin. Recombinant murine fIX (MIX), and three proteins with C‐terminal epitope tags were expressed in HEK 293 cells: tagged MIX (MIXT), tagged mouse serum albumin (MSAT) and MFUST, in which MIX and MSAT were fused in a single polypeptide chain. Proteins MFUST and MIXT were two‐ to threefold less active in clotting assays than MIX. In mice, the area under the clearance curve (AUC) was reduced for MFUST compared with MSAT or plasma‐derived MSA (pd‐MSA); the terminal catabolic half‐life (t0·5) did not differ amongst the three proteins. Two minutes after injection, >40% of the injected MFUST was found in the liver, compared with <10% of either MSAT or pd‐MSA. In rabbits, the AUC for MFUST was reduced compared to MIXT, MSAT, or pd‐MSA, while the t0·5 of the fusion protein fell between that of MIXT and MSAT or pd‐MSA. Similar results were obtained with non‐radioactive fused or non‐fused recombinant human fIX in fIX knockout mice. The clearance behaviour of the fusion protein thus more closely resembled that of fIX than that of albumin despite a modest increase in terminal half‐life, suggesting that fIX‐specific interactions that are important in determining clearance were maintained in spite of the increased size of the fusion protein.
Recessive genes controlling resistance to Helminthosporium leaf blight in synthetic hexaploid wheat
Authors: Ragiba M. , Prabhu K. V. , Singh R. B. 
Published: July 2004

AbstractA study was conducted under controlled environment conditions in a phytotron to determine the nature of the inheritance of resistance Helminthosporium leaf blight (HLB) in a synthetic hexaploid wheat line, ‘Chirya‐3’, against the isolate KL‐8 of Bipolaris sorokiniana from the major wheat growing region of India. Crosses were made between two susceptible lines ‘WH 147’ and ‘Chinese Spring’. Analyses of F1 and F2 populations of these two crosses (‘WH 147’×‘Chirya‐3’ and ‘Chinese Spring’×‘Chirya‐3’) showed that resistance against the isolate in ‘Chirya‐3’ was governed by two recessive genes functioning in a complementary interaction giving an F2 segregation pattern of 1 : 15 (resistant : susceptible). The segregation pattern of the resistant F2 progenies in F3 families from both crosses confirmed that two homozygous recessive genes were responsible for resistance to the isolate of Bipolaris sorokiniana in the synthetic line ‘Chirya‐3’. It is proposed that the genes be designated as hlbr1 and hlbr2.
Evidence of pathotypes among Australian isolates of crown rust infecting perennial ryegrass
Authors: Aldaoud R. , Anderson M. W. , Reed K. F. M. , Smith K. F. 
Published: July 2004

AbstractFifty‐three genotypes of perennial ryegrass selected from a range of cultivars and ecotypes were evaluated for their reaction to crown rust isolates collected from Western Australia, Victoria, Queensland, South Australia or New South Wales in Australia. The reactions of the clones to inoculation provided evidence for the development of a differential set of perennial ryegrass genotypes, but also demonstrated that a large number of genotypes displayed universal reactions to infection with any of the crown rust isolates.
Isolation of two microsatellite markers from BAC clones of the Vf scab resistance region and molecular characterization of scab‐resistant accessions in Malus germplasm*
Authors: Vinatzer B. A. , Patocchi A. , Tartarini S. , Gianfranceschi L. , Sansavini S. , Gessler C. 
Published: July 2004

AbstractA polymerase chain reaction (PCR)‐based method was developed to isolate microsatellite markers from large‐insert genomic DNA clones of bacterial artificial chromosome (BAC) libraries. The method is fast and economic since it does not require subcloning. It was applied to isolate a microsatellite marker from a BAC clone of the chromosomal region containing the apple scab resistance gene Vf. The Vf gene of Malus floribunda 821 is the most widely used source of scab resistance in apple breeding. A second microsatellite was found on the extremity of a BAC clone flanking the Vf locus. The two microsatellites allowed the identification of the presence of the Vf gene in the scab‐resistant accessions M. micromalus SA573‐3, ‘Golden Gem’, M. prunifolia 19651 and MA 16 not previously known to carry this gene. They were also used to verify the correctness of the published genealogical tree of the Vf cultivar ‘Florina’, in which a probable mistake was identified. This analysis shows the importance of genotyping the Vf locus when choosing scab‐resistant germplasm as parents in breeding programmes.
Screening for lodging resistance in spring wheat breeding programmes
Authors: Kelbert A. J. , Spaner D. , Briggs K. G. , King J. R. 
Published: July 2004

AbstractSelection for lodging resistance in Canadian hard red spring wheat under natural conditions is difficult due to the sporadic and often random nature of lodging events. We conducted field trials in Edmonton AB Canada (2000–2002) to determine if either a high seeding rate, or artificially inducing lodging (by dragging a weighted apparatus across plots at the early milk stage), would be an effective screen for determining genetic lodging resistance in wheat breeding programmes. For the 25 genotypes tested, applying artificial lodging at the experimental mean early milk stage was a suitable method to screen and identify lodging tolerant and susceptible genotypes. Tolerance to artificially induced lodging was mainly found within semi‐dwarf genotypes, and susceptibility was mainly found within Canadian bread wheat genotypes. Severe lodging resulted in yield losses as high as 40%. Lodging tolerant genotypes identified in this study are now being crossed into elite western Canadian bread wheat in an effort to increase genetic lodging resistance within Canadian breeding lines.
Development of molecular markers to assess the level of introgression of Populus tremula into P. alba natural populations
Authors: Fossati T. , Patrignani G. , Zapelli I. , Sabatti M. , Sala F. , Castiglione S. 
Published: July 2004

AbstractMorphological traits traditionally adopted to discriminate between Populus alba L. and P. tremula L. have frequently led to misclassifi‐cation of their spontaneous hybrid P. × canescens Sm. Moreover, they may not be of any help in cases of spontaneous backcross phenomena. These limitations can be overcome by molecular markers, which are not environmentally influenced nor subjectively assessed. In this study, the effectiveness of amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers in species and hybrid discrimination was evaluated by analysing a set of reference samples of P. alba, P. tremula and P. × canescens. Species‐specific and species‐indicative AFLPs, as well as diagnostic SSR alleles, were recorded in both P. alba and P. tremula reference samples. The results allowed a clear distinction between the two poplar species and their hybrid. Using these diagnostic markers, a natural population of P. alba trees sampled along the Ticino river basin in northern Italy was analysed, and P. × canescens individuals, intermingled with P. alba trees, were detected.
Natural outcrossing rate in Vernonia galamensis
Authors: Baye T. , Becker H. C. 
Published: July 2004

Abstract Vernonia galamensis is a potential new industrial crop growing wild in Ethiopia. The seed oil is rich in vernolic acid, an epoxy fatty acid, which is of interest for oleochemical uses. Basic information on the reproductive system of Vernonia is still very limited. The amount of natural outcrossing was estimated at two locations in Ethiopia (Alemaya and Babile) using flower colour as a marker. Single plants with white flowers, which is a monogenic recessive trait, were planted in plots with normal pink flowers and the outcrossing rate was estimated from the frequency of pink‐flowered plants in the progeny of the white‐flowered plants. Estimates of the natural outcrossing rate ranged between individual plants from 3.5 to 16% at Alemaya and 2.5 to 12% at Babile. Vernonia galamensis can be classified as a mainly self‐pollinated species.
Clinical characteristics of normotensive renal transplant recipients with microalbuminuria and effects of angiotensin II type I receptor antagonist on urinary albumin excretion
Authors: SATOH SHIGERU , HORIKAWA YOHEI , KAKINUMA HIDEAKI , TSUCHIYA NORIHIKO , WANG LIZHONG , KATO TETSURO , HABUCHI TOMONORI 
Published: July 2004

Abstract Aim: Microalbuminuria is typically observed in renal transplant recipients with systemic hypertension. The effects of angiotensin II type 1 receptor antagonist (losartan) on the hypertensive recipients have been evaluated. However, the clinical background of normotensive recipients with microalbuminuria and the effect of losartan administration in those subjects have not been clarified. One of the two purposes for the present study was to investigate the clinical characteristics of normotensive recipients with microalbuminuria. The other was to evaluate the effect of losartan on urinary excretion of albumin in these patients.Methods: The clinical data and the change of the single kidney glomerular filtration rate (GFR) for the graft by radionuclide study were assessed in 13 normotensive recipients with microalbuminuria. These were compared with the data of 13 normotensive patients without microalbuminuria. The 13 recipients with microalbuminuria were treated with losartan for one year and urine excretion of albumin, N‐acetyl‐β‐D‐glucosaminidase (NAG) and serum creatinine (S‐Cr) levels were measured.Results: The GFR of the grafts from donors to recipients significantly increased (30.9 to 55.2 mL/min) in microalbuminuric recipients, but did not significantly increase in the non‐microalbuminuric recipients. Decreases of the urinary excretion rate of albumin (351 ± 261 at baseline to 158 ± 14 mg/gCr at 12 months), NAG (13 ± 5 to 10 ± 3 IU/gCr) and S‐Cr (1.7 ± 0.6 to 1.5 ± 0.4 mg/dL) were observed in the microalbuminuric recipients with losartan administration.Conclusions: The present study suggests that an increased single kidney GFR of the graft from the donorin situto the recipient might be a cause of microalbuminuria in normotensive recipients. The one‐year effects of losartan were observed in terms of the decrease in urinary excretion of albumin, NAG and S‐Cr levels.
Effects of acupuncture for chronic pelvic pain syndrome with intrapelvic venous congestion: Preliminary results
Authors: HONJO HISASHI , KAMOI KAZUMI , NAYA YOSHIO , UKIMURA OSAMU , KOJIMA MUNEKADO , KITAKOJI HIROSHI , MIKI TSUNEHARU 
Published: July 2004

Abstract  Background:  The present study was designed to reveal the usefulness of acupuncture for chronic pelvic pain syndrome with intrapelvic venous congestion as evaluated by symptom scores, transrectal ultrasonography (TRUS) and magnetic resonance (MR) venography.Methods:  Ten male patients suffering from non‐inflammatory chronic pelvic pain syndrome (NIH category IIIB) with intrapelvic venous congestion were treated using acupuncture. Eight patients had previously received pharmacotherapy, which was unsuccessful. Acupuncture was performed using disposable stainless steel needles, which were inserted into the bilateral BL‐33 points and rotated manually for 10 min. The treatment was repeated every week for 5 weeks without other therapeutic maneuvers. Results from TRUS and MR venography, as well as clinical symptoms based on the NIH chronic prostatitis symptom index (NIH‐CPSI) and the international prostate symptom score (IPSS), were compared before and after the treatment.Results:  No side‐effects were recognized throughout the treatment period. The average pain and QOL scores of the NIH‐CPSI 1 week after the 5th acupuncture treatment decreased significantly (P < 0.05 and P < 0.01, respectively) compared with the baseline. The maximum width of the sonolucent zone 1 week after the 5th treatment also decreased significantly (P < 0.01, compared with the baseline). Intrapelvic venous congestion demonstrated by MR venography was significantly improved in four patients.Conclusion:  This study provided novel information concerning the therapeutic effects of acupuncture on non‐inflammatory chronic pelvic pain syndrome.
Urodynamic effects and safety of modified intravesical oxybutynin chloride in patients with neurogenic detrusor overactivity: 3 years experience
Authors: SAITO MOTOAKI , WATANABE TAKESHI , TABUCHI FUMIE , OTSUBO KENJI , SATOH KEISUKE , MIYAGAWA IKUO 
Published: July 2004

Abstract  Background:  Intravesical oxybutynin chloride with hydroxypropylcellulose (HPC) (modified intravesical oxybutynin) has been reported to be effective for treatment of overactive bladder. We reported the short‐term effects of modified intravesical oxybutynin previously. In the present article, we detail the results of a 3‐year follow‐up study of patients from our previous analysis and report the efficacy and side‐effects of modified intravesical oxybutynin.Methods:  Modified intravesical oxybutynin (5 mg/10 mL, twice a day) was applied for more than 3 years to six neurogenic overactive detrusor patients (three men and three women, average age 53.3 years) who were not satisfied with oral anticholinergic agents or the other therapy. A cystometogram (CMG) was performed before, 1 week after and 3 years after the start of modified intravesical oxybutynin treatment. We evaluated the patient's satisfaction of this treatment after 4 weeks and again after 3 years. We compared the patients’ answers before and after the therapy (excellent, good, fair, unchanged and worse). We also monitored systemic and topical side‐effects in these patients during this period.Results:  CMG studies showed that two of six patients no longer exhibited uninhibited contraction 1 week after the treatment and that the cystocapacity of patients before, 1 week after and 3 years after the initial modified intravesical oxybutynin was 129.7 ± 19.4, 283.5 ± 40.4 and 286.8 ± 38.1 mL, respectively. For the evaluation of patients’ satisfaction with this treatment, four patients considered the therapy excellent and one patient described it as good after both 4 weeks and after 3 years. Two patients dropped out of the study; one developed left ureteral cancer (2.25 years) and the other developed ileus (1.5 years). Dry mouth and acute cystitis were observed in both patients.Conclusion:  Modified intravesical oxybutynin is an effective and relatively safe option of therapy for overactive bladder patients. However, this therapy requires careful observation for emergent side‐effects.
Buprenorphine blocks withdrawal in morphine‐dependent rat pups
Authors: Stoller Dawn C. , Smith Forrest L. 
Published: July 2004

SummaryBackground : Infants placed on extracorporeal membrane oxygenation (ECMO) or mechanical ventilation often need continuous morphine infusions for pain relief and sedation. The resulting physical dependence requires an additional 2–3‐week hospital stay to taper the morphine to avoid withdrawal. Buprenorphine effectively blocks abstinence in dependent adults, and in infants it could accelerate or eliminate the tapering schedule, thereby enabling earlier hospital dismissals.Methods : Morphine‐dependent infant rats were used in this study to determine the effectiveness of buprenorphine in blocking abstinence. Postnatal day‐14 (P14) rats were implanted with osmotic minipumps that delivered saline (1 μl·h−1) or morphine (2 mg·kg−1 h−1) for 72 h. The minipumps were then removed to allow the rats to undergo spontaneous morphine withdrawal.Results : The withdrawal period lasted approximately 72 h out of a 96‐h observation period. The following signs were significant during these hours: wet‐dog shakes, 1–72 h; abdominal stretches, 1–72 h; forepaw tremors, 1–24 h; splayed hind‐limbs, 1–72 h; ptosis, 4–72 h; and evoked vocalization, 4 and 8 h. A single 1 mg·kg−1 buprenorphine dose significantly decreased wet‐dog shakes from 1 to 72 h, abdominal stretches from 1 to 48 h, forepaw tremors and splayed hind‐limbs 1–8 h, and ptosis and evoked vocalization at 4 and 8 h. Repeated administration of 1 mg·kg−1 buprenorphine before pump removal and at 24, 48 and 72 h resulted in a greater magnitude of blockade of abstinence throughout the 96‐h observation period.Conclusions : Buprenorphine may prove to be a suitable drug for treating opioid withdrawal in human infants.
Low doses of rocuronium during remifentanil–propofol‐based anesthesia in children: comparison of intubating conditions*
Authors: Özteki̇n Sermi̇n , Hepaǧuşlar Hasan , Ki̇lerci̇k Hakan , Kar Aysun Afi̇fe , Boyaci Fi̇gen , Elar Zahi̇de 
Published: July 2004

SummaryBackground : In this prospective double‐blind study, intubation conditions were compared at 90 s following two different low doses of rocuronium during remifentanil and propofol anesthesia in children undergoing ambulatory procedures.Methods : Forty‐four children (ASA I‐II, aged 3–12 years) undergoing day case ENT surgery were premedicated with midazolam 0.5 mg· kg−1. Following atropine 10 μg·kg−1, remifentanil infusion 0.5 μg·kg−1·min−1 was started. After 60 s, anesthesia was induced with propofol 2.5 mg·kg−1. Immediately after a bolus dose of propofol, the children received rocuronium doses of 0.15 mg·kg−1 (group I, n = 22) or 0.3 mg·kg−1 (group II, n = 22) in a randomized manner, after which an infusion of propofol 6 mg·kg−1 h−1 was added to the infusion of remifentanil 0.5 μg·kg−1 min−1 for maintenance of anesthesia. Intubating conditions were evaluated 90 s after rocuronium administration applying the Copenhagen Scoring System which included components of laryngoscopy, vocal cord movement and reaction to intubation. Hemodynamic values were recorded at predetermined time intervals.Results : Excellent, good and poor intubation conditions were 18.2, 40.9 and 40.9% in group I and 40.9, 54.5 and 4.5% in group II. Clinically acceptable intubating conditions (excellent and good) were significantly higher in group II (95.5%) than in group I (59.1%) (P = 0.004). Mean values of heart rate and blood pressure did not differ significantly between groups. No children required any intervention for hemodynamic instability and/or muscle rigidity.Conclusions : The results suggest that 0.3 mg·kg−1 of rocuronium may be a better low dose than 0.15 mg·kg−1 of rocuronium for clinically acceptable intubating conditions in pediatric ambulatory surgery during remifentanil–propofol‐based anesthesia at the doses used in the study.
Propofol or propofol–alfentanil anesthesia for painful procedures in the pediatric oncology ward
Authors: von Heijne Margareta , Bredlöv Britt , Söderhäll Stefan , Olsson Gunnar L. 
Published: July 2004

SummaryBackground : For children with cancer receiving curative treatment, the pain of diagnostic and therapeutic procedures is often worse than that of the disease itself.In order to evaluate if light propofol anesthesia in the pediatric oncology ward (POW) could improve the management of procedure pain and anxiety, a questionnaire was developed.Methods : After prolonged EMLA application, 65 propofol anesthetics were performed successfully in 28 children during lumbar puncture and/or bone marrow aspiration in the POW, with short recovery time and without major adverse events. The questionnaire was mailed to the parents of the 28 children who were included in the survey.Results : The return of questionnaire compliance was 89% (25 of 28), 12 females and 13 males, mean age was 7 years (range 2–16). Among those who replied, the diagnoses were acute lymphatic leukemia in 21, lymphoma in two and tumor in the other two. In the questionnaire, all parents/patients reported advantages with anesthesia in the POW compared with the operating room. In the list of stated advantages, 88% marked ‘familiar nurses and doctors’, 84%‘familiar environment’, 80%‘closer to own room’, 68%‘the child more calm’, 72%‘shorter waiting‐time’, 60%‘faster recovery’, 44%‘shorter fasting‐time’ and 44%‘parents more calm’, as benefits. For future procedures requiring anesthesia to reduce pain, discomfort and/or anxiety, 92% of the parents/patients preferred anesthesia in the POW.Conclusions : If anesthesia is chosen for invasive procedures, this study suggest that propofol anesthesia in the POW is preferred by parents and children.