The effect of Protandim® supplementation on athletic performance and oxidative blood markers in runners
Authors: Zavorsky Gerald S. , Ueberschlag Seteena L. , Seay James R. , Roberts Alexandra H. , DeSpirito Pamela C. , Stith Jeremy M. , Folz Rodney J. , Carter Kathleen A. , Weiss Edward P. 
Published: November 2023

BackgroundRegular exercise training can enhance antioxidant defense mechanisms. Supplements that may further increase these antioxidant defense mechanisms could be of value to athletes. Protandim® is a nutraceutical made of 5 plant extracts. One pill contains 225 mg milk thistle, 150 mg bacopa extract, 150 mg ashwagandha, 75 mg turmeric, and 75 mg of green tea, which has been shown to increase antioxidant enzymes such as superoxide dismutase (SOD), catalase, hemoxygenase and gluthathione.ObjectiveTo determine whether taking Protandim® orally for 90 days would improve 5‐km running performance in regional‐class runners. A secondary objective was to look at blood anti‐oxidant enzymes [glutathione (GSH), glutathione peroxidase (GPX), and SOD] and serum thiobarbituric acid‐reacting substances (TBARS) as an indicator of oxidative stress at rest and 10 minutes after the completion of the run.DesignIn a double‐blind, randomized, placebo‐controlled trial, 38 healthy runners [mean (SD) = 34 (7) yrs; BMI = 22 (2) kg/m2] were randomized to receive 90 days of Protandim® (1 pill a day) or placebo. A 5‐km race was performed at baseline and after 90 days of supplementation, with blood samples being acquired before and 10‐min after each race. Fasting (non‐exercise) blood samples were acquired at baseline and after 30, 60, and 90 days of supplementation. Race time, serum GSH, GPX, SOD and TBARS were analyzed using a repeated‐measures ANOVA.ResultsRunning performance was not altered by Protandim® or placebo supplementation [20.3 (2.1) minutes, with an −8 (33) seconds change in 5‐km time regardless of group, p=0.84 between groups]. There was no statistically significant change in fasting or pre‐post run serum SOD, GSH, or GPX overtime, within or between groups (all p>0.05). Mean fasting serum TBARS were normal [8.1 (2.0) μmol/L] and remained unchanged with Protandim® or placebo supplementation. The post‐race change in TBARS (compared to pre‐race) was not altered between groups, even after 88 days of supplementation [Protandim®, post ‐ pre change = +0.1 (1.7) μmol/L prior to supplementation, +0.1 (1.2) μmol/L after 88 days of Protandim®; Placebo, post ‐ pre change = +0.5 (1.9) μmol/L prior to supplementation, −0.9 (4.8) μmol/L after 88 days of placebo] (all p>0.05).ConclusionRegular supplementation with Protandim® did not alter 5‐km running performance. Protandim® supplementation did not statistically change blood antioxidant enzymes in these runners. As a whole, the 5‐km race did not induce oxidative stress as seen by serum TBARS which remained normal (<10 μmol/L). Further studies are warranted to examine intracellular levels of antioxidants or mitochondrial function in these type of subjects rather than measuring serum free radical load and antioxidants levels.ClinicalTrials.gov Identifier: NCT02172625Support or Funding InformationThis study was supported by an industry grant from LifeVantage Corporation