bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is poor dietary quality in adolescence a risk factor for painful temporomandibular disorders and headaches in young adulthood? A prospective study in the Danish National Birth Cohort
Authors: Mansoori Mojdeh , Rocha Exposto Cristina , Hammer Bech Bodil , Frodi Olsen Sjurdur , Ahrendt Bjerregaard Anne , Baad‐Hansen Lene 
Published: February 2025

AbstractObjectiveTo investigate the association between adolescents’ dietary quality and the presence of painful temporomandibular disorder (p‐TMD) and headaches in young adulthood.BackgroundP‐TMD is a common orofacial pain condition often associated with headaches and discomfort. Some studies have shown that dietary behaviors can impact chronic musculoskeletal pain. Although various factors such as sex, age, psychosocial aspects, and pain sensitivity contribute to p‐TMD, the role of nutrition remains unclear.MethodsThe dietary quality of 32,247 singletons from the Danish National Birth Cohort (DNBC) at age 14 was assessed using the Healthy Eating Index (HEI) encompassing eight domains. Among these, 11,982 (37.1%) individuals completed the TMD pain screener and headache‐related queries at age 18 and above. HEI and dietary domains were analyzed as potential risk factors for p‐TMD and headaches.ResultsP‐TMD was present in 3163 of the 11,982 members of the study population. HEI scores were divided into quartiles, with quartile four indicating the highest dietary quality. Quartile four showed a higher odds ratio (OR) for p‐TMD than quartile one (OR = 1.14 [95% confidence interval (CI), 1.01–1.29]), but the significance was lost after adjustment for confounders (adjusted OR [aOR] = 1.12 [95% CI, 0.97–1.30]). Overall dietary quality was not significantly associated with headaches. However, specific dietary quality domains, such as dietary fibers (aOR = 0.97 [95% CI, 0.95–0.99]), fish (aOR = 0.98 [95% CI, 0.97–1.00]), sodium (aOR = 1.03 [95% CI, 1.01–1.06]), and added sugar (aOR = 0.97 [95% CI, 0.95–0.99]) were associated with headaches but not with p‐TMD after adjustment.ConclusionOverall adolescent dietary quality did not significantly associate with p‐TMD or headaches in young adulthood after adjusting for confounders. However, specific dietary domains exhibited weak but statistically significant associations with headaches. These findings underscore the interplay between diet and pain, calling for further research to unveil the underlying pathophysiological mechanisms connecting lifestyle, p‐TMD, and headaches.
The impacts of climate and the extreme drought in 2018 on population growth in Swedish moth species
Authors: Betzholtz Per‐Eric , Forsman Anders , Franzén Markus 
Published: February 2025

Abstract
Insects are pivotal to ecosystem diversity and functionality, yet they face increasing threats from anthropogenic climate change impacts. A growing body of studies reports the effects of changing temperature and precipitation patterns, but relatively few studies focus on the consequences for insect populations because of extreme weather events.
Here, we examine population growth responses to temperature, precipitation and the extreme summer drought in Sweden in 2018. For this purpose, we used longitudinal data for 54 moth species collected between 2005 and 2023 using light traps at three sites in southeastern Sweden.
We found a positive relationship between temperature and population growth rates across all study sites, while precipitation showed a positive relationship at two sites and no effect at the third. The results indicated a negative time‐lag effect on population growth, at two of the sites, of precipitation the previous year, while there were no significant effects of temperature the previous year. Despite the extreme drought in 2018, moth populations remained resilient, with no dramatic decline in population growth between 2018 and 2019.
Our results contrast earlier studies reporting severe declines in population growth in response to extreme drought events. The discrepancy may reflect a combination of region‐specific effects of extreme weather events and that selected species in this study predominantly consist of range‐expanding and migratory species, better able to withstand adverse conditions due to a higher climatic tolerance and being habitat and food plant generalists.
Our findings underscore the importance of an increased knowledge of site‐specific responses and effects of extreme weather events such as droughts when outlining conservation efforts.
Effect of calcium magnesium aluminate on microstructural distribution of SiO2 impurity in magnesia
Authors: Zhou Siqi , Wei Yaowu , Yu Yiming , Wöhrmeyer Christoph , Gao Jianying , Liu Chunfeng 
Published: February 2025

AbstractThe effect of calcium magnesium aluminate (hereinafter referred to as CMA) on the microstructural distribution of the main impurity SiO2 in magnesia was investigated in this research. The XRD, SEM, and EDS analyses were used in this work. It can be seen from the result that SiO2 in low‐grade sintered magnesia diffused into CMA area at high temperature and the distribution of SiO2 in sintered magnesia was significantly reduced. The main mineral phase of sintered magnesia was periclase and monticellite, and impurities in sintered magnesia mainly existed in the form of monticellite, which was completely decomposed into liquid phase and periclase above 1400°C, SiO2 in the liquid phase in the sintered magnesia migrated into the liquid phase that formed in the CMA. Furthermore, the adsorption energy of CaO on SiO2 is stronger than that of MgO on SiO2, so the SiO2 in sintered magnesia diffused into CMA due to its relatively higher CaO content compared with the sintered magnesia. The SiO2 content in fused magnesia was lower than that of sintered magnesia and the dicalcium silicate (C2S) content in fused magnesia was higher than that of sintered magnesia, so the influence of CMA on microstructural distribution of SiO2 in fused magnesia was obviously different than that with low grade sintered magnesia.
A Structural Voyage Toward the Landscape of Humoral and Cellular Immune Escapes of SARS‐CoV‐2
Authors: Liu Jun , Wu Yan , Gao George F. 
Published: February 2025

ABSTRACTThe genome‐based surveillance of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in the past nearly 5 years since its emergence has refreshed our understanding of virus evolution, especially on convergent co‐evolution with the host. SARS‐CoV‐2 evolution has been characterized by the emergence of sets of mutations that affect the functional properties of the virus by altering its infectivity, virulence, transmissibility, and interactions with host immunity. This poses a huge challenge to global prevention and control measures based on drug treatment and vaccine application. As one of the key evasion strategies in response to the immune profile of the human population, there are overwhelming amounts of evidence for the reduced antibody neutralization of SARS‐CoV‐2 variants. Additionally, data also suggest that the levels of CD4+ and CD8+ T‐cell responses against variants or sub‐variants decrease in the populations, although non‐negligible cross‐T‐cell responses are maintained. Herein, from the perspectives of structural immunology, we outline the characteristics and mechanisms of the T cell and antibody responses to SARS‐CoV and its variants/sub‐variants. The molecular bases for the impact of the immune escaping variants on the interaction of the epitopes with the key receptors in adaptive immunity, that is, major histocompatibility complex (MHC), T‐cell receptor (TCR), and antibody are summarized and discussed, the knowledge of which will widen our understanding of this pandemic‐threatening virus and assist the preparedness for Pathogen X in the future.
EZH2‐Mediated PHF10 Suppression Amplifies HMGB1/NF‐κB Axis That Confers Chemotherapy Resistance in Cholangiocarcinoma
Authors: Yin Qiushi , Lin Daning , Zeng Weiqian , Gu Shijing , Zhu Chuangshi , Liang Changfu , Yang Yan 
Published: February 2025

ABSTRACTChemoresistance represents a major threat to the treatment of human cancers, including cholangiocarcinoma (CHOL). Aberrant epigenetic events contribute most to the progression of CHOL and chemotherapy efficacy. PHF10, one subunit of SWI/SNF complex, expressed highly in tumours that correlated with tumorigenesis. However, the roles of PHF10 in CHOL remains unclear. Here, we utilised the bioinformatic analysis to reveal that PHF10 expressed lowly in CHOL samples relative to normal tissues. Functionally, we demonstrated that PHF10 deficiency enhanced cell proliferation, migration and self‐renewal capacities of CHOL cells. PHF10 ablation further enhanced the chemoresistance of CHOL cells. The transcriptome analysis revealed that PHF10‐KO could notably alter several oncogenic crosstalk, including the NF‐kB signalling. As the top hit, HMGB1 mRNA expressions had the sharpest increase upon PHF10 deficiency. PHF10 coordinated with Setdb1 to mediate the H3K9me3 modifications on the HMGB1 promoter to suppress its expressions. Low PHF10 relied on HMGB1 to promote the progression of CHOL cells in vitro and in vivo. Furthermore, EZH2 mediated the H3K27me3 enrichment on the PHF10 promoter region that contributes to its low expressions. Lastly, the HMGB1 inhibitor (Glycyrrhizin) decreased proliferation rate of PHF10‐deleted cells in vitro and in vivo. Targeting HMGB1 rendered PHF10low CHOL re‐sensitive to chemotherapy. Collectively, this study demonstrated that PHF10 functions as a tumour suppressor in CHOL, and is a novel target to predict and overcome chemoresistance.
Efficacy and safety of premixed versus basal‐bolus regimens as intensification of insulin therapy in patients with type 2 diabetes mellitus: A systematic review and meta‐analysis of randomized clinical trials
Authors: Dehghani Mohsen , Sadeghi Masoumeh , Barzkar Farzaneh , Khamseh Mohammad Ebrahim , Torshizian Ashkan , Baradaran Hamid Reza 
Published: February 2025

ABSTRACTAimTo estimate the efficacy and safety of the basal‐bolus and premixed insulin as intensification regimens in patients with type 2 diabetes mellitus (T2DM).MethodsA comprehensive search of online databases was performed until December 2022 to identify randomized controlled trials (RCTs) comparing premixed insulin versus basal‐bolus regimen with treat‐to‐target intention. The Cochrane ROB‐2 tool and GRADE approach were used for quality assessment and certainty of the evidence, respectively. Pooled weighted mean difference (WMD) and odds ratio (OR) were calculated using random‐effects meta‐analysis models.ResultsEighteen RCTs were included in the meta‐analysis, and 66% had a low risk of bias. We found no significant difference between the two regimens regarding HbA1c reduction (WMD: 0.03% [−0.05%, 0.10%]). The basal‐bolus regimen improved fasting plasma glucose (FPG) more than the premixed regimen (WMD: 6.35 mg/dL [0.31, 12.39]). Both had similar effects on weight gain. The odds of developing overall, nocturnal, and severe hypoglycemia were comparable (pooled OR: 0.9, 1.02, and 1.00, respectively) with no heterogeneity. Findings of the model were robust. The certainty of the evidence was moderate to high for all outcomes except FPG.ConclusionsTwo regimens are clinically comparable. Patient preference should be considered when adopting an individualized approach in a real‐world setting.
Establishment and Application of a Rapid Detection Method of Cyprinid Herpesvirus 2 (CyHV‐2) in Aquacultural Waters by Using a Novel One‐Pot RAA‐CRISPR/Cas12a Combined With Fe‐Iron Flocculation Technology
Authors: Hou Zewei , Ding Zhaoyang , Zhang Ye , Pang Binghui , Gui Lang , Liu Ying , Lu Liqun , Roengjitd Patarida , Wang Hao 
Published: February 2025

ABSTRACTCyprinid herpesvirus 2 (CyHV‐2) poses a substantial global threat to goldfish (Carassius auratus) and crucian carp (Carassius carassius). Despite the development of several sensitive molecular diagnostic techniques, there is an ongoing demand for alternative visualisation platforms to streamline the workflow, enhance safety profiles, and improve accessibility for end‐users. In this study, we have integrated recombinase‐aided amplification (RAA) technology with the CRISPR/Cas12a system to establish a rapid diagnostic system for CyHV‐2, termed one‐pot RAA‐CRISPR/Cas12a. This method enables the results of detection within 60 min. The RAA‐CRISPR/Cas12a platform is capable of detecting as few as 10 copies of CyHV‐2 per reaction cycle without exhibiting cross‐reactivity with other pathogens. The positive detection rate in clinical samples exceeds that of conventional PCR approaches, underscoring its high precision. Furthermore, the method could be used in conjunction with iron flocculation for the concentration and detection of viruses within aquaculture settings. This approach minimises the dissection of aquatic organisms, thereby maximising animal welfare and bolstering detection efficiency. Collectively, our findings validate the RAA‐CRISPR/Cas12a method as a robust, specific, confirmatory, user‐friendly and promising approach for on‐site diagnosis of CyHV‐2.
“By the Time We Knew …”: Poetic Analysis of End‐of‐Life Caregiving Experiences for Rapidly Progressive and Slower‐Duration Dementia Syndromes
Authors: Harrison Krista L. , Morgan Brianna E. , Friend Juliana , Garrett Sarah B. , Looi David , Halim Madina , James Jennifer E. , Boyd Nicole D. , Gilissen Joni , Geschwind Michael D. , Ritchie Christine S. , Smith Alexander K. 
Published: February 2025

ABSTRACTBackgroundOne in three older adults in the United States dies with or from dementia. Little is known about whether end‐of‐life caregiving experiences differ by dementia diagnosis.MethodsWe conducted a secondary analysis of two qualitative studies. Participants included caregivers of decedents with “rapid‐type” sporadic Creutzfeldt–Jakob Disease (sCJD, survival prognosis of < 1 year) or “slow‐type” Alzheimer's disease and related dementias (survival prognosis of 5–20 years). We used reflexive thematic analysis and a novel method, poetic analysis, to compare end‐of‐life caregiving experiences.Results“Rapid‐type” caregivers (n = 12) had a median age of 59 (range 45–73) years; 6 were female, and 9 were spouses. “Slow‐type” caregivers (n = 15) had a median age of 69 (45–82) years; 9 were female, and 11 were spouses. We identified three main areas of differential experience that were influenced by syndrome rarity and participation in research yet hinged on time. Time enables preparation: Due to the rarity of sCJD, “rapid‐type” caregivers struggled to obtain accurate diagnoses, which prevented preparation for end‐of‐life care. Weeks or months before death, specialists simultaneously disclosed sCJD diagnoses and recommended hospice. In contrast, for “slow‐type” dementia, preparation began years before death. Time complicates conflict: Most “rapid‐type” caregivers described conflicts, rarely resolved before death, about code status, treatment, or care location decisions. Fewer “slow‐type” caregivers experienced such conflicts, and these were typically resolved before death; instead, they experienced conflict between needs and what the care system provides. Postmortem experience contrasts with perimortem: For “rapid‐type” dementia, short perimortem periods contrasted with elongated and often intense postmortem logistics and grief. For “slow‐type” caregivers, preparation and perimortem grieving typically led to shorter duration and minimally intrusive postmortem logistics and grief.ConclusionsEnd‐of‐life care for dementia should attend to and support axes of differential experience based on diagnosis and rarity, time since symptom onset (affecting preparation and conflict resolution), and participation in research studies.
Association of Perinatal Factors and Family Sociodemographic Characteristics With Cardiometabolic Health in Pre‐Adolescence: Cross‐Sectional Results From Healthy Growth Study
Authors: Nakaki Ioanna , Kontochristopoulou Aikaterini M. , Anastasiou Costas , Moschonis George , Halilagic Anela , Cao Yingting , Karaglani Eva , Manios Yannis 
Published: February 2025

ABSTRACTAimStudy the influence of a variety of perinatal factors on the presence of metabolic syndrome and its parameters in prepubertal age, considering socio‐demographic factors, sex, Tanner stage and weight status of the preadolescent.MethodsData collected from the cross‐sectional Healthy Growth Study (2007) in students from four prefectures of Greece and their families. Cardiometabolic risk factors (waist circumference, blood pressure and blood indices) and perinatal data (from parents and health records) were collected. Single and a multiple logistic regression performed to assess the associations with cardiometabolic risk in preadolescence. Dependent variables included waist circumference, blood glucose, HDL‐C, TG, blood pressure and metabolic syndrome presence, while independent variables encompassed perinatal factors categorised as pre‐pregnancy, during and post‐pregnancy.Results2666 pre‐adolescents (mean age 11.2 year) participated in the study. After adjustment for parental educational level, family income, sex, Tanner stage and weight category, maternal pre‐pregnancy weight was positively associated with high waist circumference (OR, 95% CI 1.02 (1.00–1.04)), low HDL‐C levels (OR, 95% CI 1.01 (1.00–1.03)) and metabolic syndrome (OR, 95% CI 1.02 (1.00–1.05)). Timing of solid foods' introduction was positively associated with high waist circumference (OR, 95% CI 1.09 (1.01–1.08)), gestational age with high glucose levels (OR, 95% CI 1.12 (1.04–1.19)), smoking (OR, 95% CI 1.07 (1.00–1.14)) and alcohol consumption (OR, 95% CI 2.35 (1.05–5.27)) during the first trimester of breastfeeding with high glucose levels and low HDL‐C levels, respectively. Number of previous miscarriages was positively associated with low HDL‐C levels (OR, 95% CI 1.28 (1.04–1.57)). Gestational age was negatively associated with high waist circumference (OR, 95% CI 0.87 (0.80–0.95)) and iron supplementation in the third pregnancy trimester negatively association with high TG levels (OR, 95% CI 0.34 (0.12–0.91)).ConclusionsMaternal weight, perinatal behaviours, infant feeding and miscarriages influence pre‐pubertal cardiometabolic health, independent of sociodemographics, sex, Tanner stage and weight.
Identification of Plant Species Using Convolutional Neural Network with Transfer Learning
Authors: Arun Anupama , Sharma Sanjeev , Singh Bhupendra , Hazra Tanmoy 
Published: February 2025

ABSTRACTPlants are the building blocks of nature and human beings. However, the excessive explosion of population and climate changes, some plants are extinct, and some are on the corner of extinction. Additionally, numerous species remain unexplored till now. Exploring the species in the traditional way are labor‐intensive, time‐consuming and require specialised expertise. So, it is a very challenging task. To overcome these challenges, various state‐of‐the‐art approaches have been proposed. These approaches often face significant limitations related to accuracy, training and testing processes. This paper proposed a novel approach to species identification leveraging deep learning techniques, employing a weighted average methodology. The proposed approach utilises well known publicly available datasets like Malayakew (MK) and Leafsnap, to evaluate F1 score, recall, accuracy, and precision. In proposed approach we utilised pretrained Convolutional Neural Networks (CNNs) and Transfer Learning (TL) to enhance performance. Specifically, architectures such as NASNet, DenseNet121, ResNet50V2, Xception, VGG19 and VGG16 were employed in the experimental study. The proposed approach achieved an F1 score of 99.9%, recall of 100%, accuracy of 100% and precision of 100% on the MK dataset. On the Leafsnap dataset, the suggested approach achieved an F1 score of 94%, recall of 94%, accuracy of 93.5% and precision of 94%. These results demonstrate that the proposed approach significantly outperforms existing state‐of‐the‐art works, offering a robust and efficient solution for species identification across diverse datasets.
Damsels in Disguise: Development of Ultraviolet Sensitivity and Colour Patterns in Damselfishes (Pomacentridae)
Authors: Tettamanti Valerio , Marshall N. Justin , Cheney Karen L. , Cortesi Fabio 
Published: February 2025

ABSTRACTDamselfishes (Pomacentridae) are widespread and highly abundant on tropical coral reefs. They exhibit diverse body colouration within and between the ~250 species and across ontogenetic stages. In addition to human‐visible colours (i.e., 400–700 nm), most adult damselfishes reflect ultraviolet (UV, 300–400 nm) colour patches. UV sensitivity and UV colour signals are essential for feeding and form the basis for a secret communication channel invisible to the many UV‐blind predatory fish on the reef; however, how these traits develop across ontogenetic stages and their distribution across the damselfish family is poorly characterised. Here, we used UV photography, phylogenetic reconstructions of opsin genes, and differential gene expression analysis (DGE) of retinal samples to investigate the development of UV vision and colour patterns in three ontogenetic stages (pre‐settlement larval, juvenile, and adult) of 11 damselfish species. Using DGE, we found similar gene expression between juveniles and adults, which strongly differed from larvae. All species and all stages expressed at least one UV‐sensitive sws1 opsin gene. However, UV body colour patterns only started to appear at the juvenile stage. Moreover, Pomacentrus species displayed highly complex UV body patterns that were correlated with the expression of two sws1 copies. This could mean that some damselfishes can discriminate colours that change only in their UV component. We demonstrate dramatic shifts in both UV sensitivity and UV colouration across the development stages of damselfish while highlighting the importance of considering ontogeny when studying the coevolution of visual systems and colour signals.
Volumetric Assessment of Resorption Patterns of Bilateral Alveolar Clefts in Cone‐Beam Computed Tomography in Two‐ Stage Bone Graft
Authors: Regnstrand Tobias , Bousiou Alice , Karsten Agneta , Benchimol Daniel , Jacobs Reinhilde 
Published: February 2025

ABSTRACTObjectiveFew studies have analysed the outcome of bone grafts in bilateral alveolar clefts and the bone fill with a two‐step surgery method. The currently applied three‐dimensional method used in this study enables a comprehensive description of the bone fill of bilateral clefts after bone grafting. The study aimed to describe alveolar cleft volume and bone fill after alveolar bone grafting of bilateral alveolar clefts treated with two‐step bone grafting, with a comparison between the first and the second bone graft site. A secondary aim was to investigate whether the cleft volume on the non‐surgical side changed after contralateral surgery.Materials and MethodsIn this retrospective study, 60 CBCT scans from 20 patients were included (8 girls and 12 boys) with an age range of 6.5–11.5 years (mean age 8.7). The cleft volume was measured in pre‐ and post‐operative CBCT scans and assessed in ITK‐SNAP to calculate the bone fill of the cleft.ResultsAfter bone grafting, 47% of the first bone‐grafted cleft was filled with bone, and 33% of the second bone‐grafted cleft, without significant difference between them (p = 0.03). The mean preoperative cleft volume was 0.42cm3 and the mean residual cleft volume after bone graft was 0.23cm3. There was however a significant difference when comparing the bone fill between the nasal and the dental part (p < 0.001).ConclusionAlmost half of the cleft volume was filled with bone after bone grafting. The order of the bone graft side did not influence the bone fill of the cleft.