bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intramural hematoma in the proximal sealing zone of the thoracic endovascular aneurysm repair: frequency and safety in acute and subacute type B dissections
Authors: Lescan Mario , Mustafi Migdat , Hahn Julia , Schlensak Christian , Andic Mateja 
Published: November 2023

IntroductionTo assess the outcomes after thoracic endovascular aneurysm repair (TEVAR) in the presence of intramural hematoma (IMH) in the proximal sealing zone.Material and methodsPatient data were retrospectively extracted from the hospital records of patients treated with TEVAR for acute and chronic aortic dissection type B in one single center. The initial, preoperative, first postoperative, and last follow-up CT scans were evaluated in the aortic 3D multiplanar reformats and the centerline regarding IMH presence in the proximal sealing zone, anatomical preconditions, and the morphological TEVAR complications including migration and bird-beak. Groups with (IMH) and without IMH (no-IMH) were compared.ResultsOverall, 84 patients (IMH:42; no-IMH:42) were treated at the age of 63(55; 72) years, of whom 23/84 (27%), 34/84 (40%), and 27/84 (32%) were in the hyperacute, acute and subacute dissection phases, respectively. The bovine arch was found in 10/84(12%) and the type III arch was most common (43/84;51%). IMH maximum extent was found in zones 0, 1, 2, and 3 in 14/84 (17%), 17/84 (20%), 18/84 (21%), and 6/84 (7%), respectively. Sealing was achieved in zone II in 71/84 (85%) and LSA was revascularized in 66/84 (79%) of the overall cohort. Early mortality and paraplegia were 2/84 (2%) each; stroke rate was 3/84 (4%). During the 22 months median follow-up (22;4;43) no RTAD was observed. Migration ≥10 mm (IMH: 11/82; no-IMH: 10/82; P = 1.0) and bird-beaks (IMH: 10/82; no-IMH: 12/82; P = 0.8036) were comparable in both groups and accompanied by a low aorta related mortality (1/82) in both groups.ConclusionThe presence of the IMH in the proximal TEVAR sealing zone is frequent and may not be relevant for the occurrence of the RTAD, stent-graft migration, or bird-beak formation.
Case Report: Flurbiprofen-induced Type I Kounis syndrome
Authors: Tang Chao , Chen Yuqi , Gu Xiaosong 
Published: November 2023

BackgroundKounis syndrome is a specific type of acute coronary syndrome caused by allergic or hypersensitivity response. Clinical knowledge about this syndrome is insufficient. We report a case in which intravenous administration of flurbiprofen resulted in Type I Kounis syndrome.Case summaryA 60-year-old female patient with no history of coronary artery disease developed limb erythema, hypotension, and chest tightness after receiving intravenous flurbiprofen. Electrocardiogram showed ST segment elevation in leads II, III, and aVF. Emergency coronary angiography revealed no significant stenosis or thrombus in the coronary arteries. Subsequent echocardiography showed no apparent abnormalities. Levels of troponin T were elevated. The diagnosis was flurbiprofen-induced Type I Kounis syndrome, presenting as acute ST segment elevation myocardial infarction.ConclusionsPatients with Kounis syndrome can exhibit severe clinical symptoms, and their condition may even be life-threatening. It is important for clinicians to have a thorough understanding of this syndrome in order to develop comprehensive treatment plans.
Total Astragalus saponins can reverse type 2 diabetes mellitus-related intestinal dysbiosis and hepatic insulin resistance in vivo
Authors: Ma Leilei , La Xiaojin , Zhang Biwei , Xu Wenxuan , Tian Chunyu , Fu Qianru , Wang Meng , Wu Chenxi , Chen Zhen , Chang Hong , Li Ji-an 
Published: November 2023

ObjectiveIntestinal flora homeostasis in rats with type 2 diabetes mellitus (T2DM) was evaluated to explore the effects of total Astragalus saponins (TAS) on hepatic insulin resistance (IR).MethodsSix-week-old male Sprague–Dawley rats were fed high-fat and high-sugar diet for 4 weeks and intraperitoneally injected with streptozotocin to induce T2DM, and they were then randomly divided into control, model, metformin, and TAS groups. Stool, serum, colon, and liver samples were collected after 8 weeks of drug administration for relevant analyses.ResultsTAS reduced fasting blood glucose, 2-hour postprandial blood glucose, area under the curve of oral glucose tolerance test, glycated serum protein, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels in T2DM rats but increased insulin, C-peptide, and high-density lipoprotein cholesterol levels. Moreover, TAS improved the morphology and structure of liver and colon tissues and improved the composition of the intestinal microbiome and bacterial community structure at different taxonomic levels. In addition, TAS increased the protein expression of hepatic IRS-1, PI3K, PDK1, and p-AKT and decreased the protein expression of p-GSK-3β. Meanwhile, TAS increased the mRNA expression of liver PDK1, PI3K, and GS and decreased the mRNA expression of GSK-3β.ConclusionTAS can ameliorate T2DM-related abnormal glucose and blood lipid metabolism, intestinal dysbiosis, and IR.
The distribution and metabolism of hexabromocyclododecane isomers varies in the tissues of Nibea albiflora
Authors: Song Suping , Sun Xiumei , Cheng Xin , Peng Xijian , Hao Qing , Hu Hongmei , Zhu Jian , Li Tiejun , Guo Yuanming 
Published: November 2023

Hexabromocyclododecanes (HBCDs), as brominated flame retardants, have increasingly drawn concern due to their detection in various marine organisms in recent years. The present test investigated the selective accumulation, depuration, and bioisomerization of HBCDs in different tissues and organs of Nibea albiflora, as well as the genetic metabolic behavior of HBCDs between parents and offspring. In a semi-static water environment, N. albiflora were exposed to individual HBCD diastereoisomers (α-HBCD, β-HBCD, or γ-HBCD) at a concentration of 0.4 μg/L for bioaccumulation, followed by clean food for depuration. During the exposure period, the highest concentrations of these HBCDs were observed in the intestine and gill of N. albiflora. α-HBCD was detected in all exposure groups, suggesting that both β-HBCD and γ-HBCD could be bioisomerized to α-HBCD in N. albiflora. In addition, there was no bioisomerization of α-HBCD to either β-HBCD or γ-HBCD, and there was no bioisomerization between β-HBCD and γ-HBCD. The half-lives (t1/2) were variable among different diastereoisomers, following an order of α-HBCD &gt; γ-HBCD &gt; β-HBCD. The most rapid depuration rate of HBCDs was discovered in the liver while muscle showed the least. Additionally, prior to spawning, the parent fish were exposed to contaminated feed (silkworms sprayed with 200 μL of a 30 μg/mL HBCD solution) for 6 days. By comparing the HBCDs concentrations in the maternal gonad, fertilized eggs, hatching eggs, larvae, and juveniles, it was determined that the maternal transfer coefficients for HBCDs were approximately 1, indicating dynamic equilibrium of HBCDs throughout the process from maternal tissue to progeny development. Differences in HBCDs pattern between organs observed in the experiment support a proposal of an organ-specific diastereomer accumulation.
Exploring causal correlations between inflammatory cytokines and ankylosing spondylitis: a bidirectional mendelian-randomization study
Authors: Fang Peng , Liu Xiaozhou , Qiu Yang , Wang Yang , Wang Dongsheng , Zhao Jianning , Ding Hao , Bao Nirong 
Published: November 2023

BackgroundThe impact of inflammatory factors on the development of Ankylosing Spondylitis (AS) is widely recognized, but the exact causal relationship remains unclear.MethodsThe bidirectional mendelian-randomization study utilized genetic data from a genome-wide association study (GWAS) of 186 AS cases and 456,162 controls of European ancestry. Inflammatory cytokines were obtained from a GWAS summary of 8,293 healthy participants. Causal associations were primarily investigated using the inverse variance-weighted method, supplemented by MR Egger, weighted median and weighted mode analyses. Heterogeneity in the results was assessed using the Cochrane Q test. Horizontal pleiotropy was evaluated through the MR-Egger intercept test and the MR pleiotropy residual sum and outliers (MR-PRESSO) test. Sensitivity analysis was conducted through leave-one-out analysis.ResultsThe results suggest a genetically predicted potential association between beta-nerve growth factor (βNGF), Interleukin-1-beta (IL-1β), and TNF-related apoptosis inducing ligand (TRAIL) with the risk of AS (OR: 2.17, 95% CI: 1.13-4.16; OR: 0.41, 95% CI: 0.18-0.95,; OR: 1.47, 95% CI: 1.02-2.13).Additionally, Interleukin-12p70 (IL-12p70), Interleukin-17 (IL-17), Interleukin-6 (IL-6), Interleukin-4 (IL-4), Stromal-cell-derived factor 1 alpha (SDF−1α), Macrophage inflammatory protein 1β (MIP1β), Monocyte chemoattractant protein-3 (MCP-3), Platelet-derived growth factor bb (PDGFbb), Granulocyte-colony stimulating factor (GCSF), Fibroblast growth factor basic (bFGF), TNF-related apoptosis inducing ligand (TRAIL), and Interferon-gamma (IFN -γ) are suggested as consequences of AS in genetically prediction.No evidence of horizontal pleiotropy or heterogeneity between the genetic variants was found (P&gt;0.05), and a leave-one-out test confirmed the stability and robustness of this association.ConclusionThese findings suggest that βNGF, IL-1β, and TRAIL may play a crucial role in the pathogenesis of AS. Additionally, AS may impact the expression of cytokines such as IL-12p70, IL-17, IL-6, IL-4, SDF−1α, MIP1β, MCP-3, PDGFbb,GCSF, bFGF,TRAIL,and IFN-γ. Further investigations are warranted to determine whether these biomarkers can be utilized for the prevention or treatment of AS.
Identification of omega-3 oxylipins in human milk-derived extracellular vesicles with pro-resolutive actions in gastrointestinal inflammation
Authors: Gómez-Ferrer Marta , Amaro-Prellezo Elena , Albiach-Delgado Abel , Ten-Domenech Isabel , Kuligowski Julia , Sepúlveda Pilar 
Published: November 2023

IntroductionPremature infants (PIs) are at risk of suffering necrotizing enterocolitis (NEC), and infants consuming human milk (HM) show a lower incidence than infants receiving formula. The composition of HM has been studied in depth, but the lipid content of HM-derived small extracellular vesicles (HM sEVs) remains unexplored. Identifying these molecules and their biological effects has potential for the treatment of intestinal disorders in PIs and could contribute to the development of HM-based fortified formulas.MethodsWe isolated HM sEVs from HM samples and analyzed their oxylipin content using liquid chromatography coupled to mass spectrometry, which revealed the presence of anti-inflammatory oxylipins. We then examined the efficacy of a mixture of these oxylipins in combating inflammation and fibrosis, in vitro and in a murine model of inflammatory bowel disease (IBD).ResultsHM-related sEVs contained higher concentrations of oxylipins derived from docosahexaenoic acid, an omega-3 fatty acid. Three anti-inflammatory oxylipins, 14-HDHA, 17-HDHA, and 19,20-DiHDPA (ω3 OXLP), demonstrated similar efficacy to HM sEVs in preventing cell injury, inducing re-epithelialization, mitigating fibrosis, and modulating immune responses. Both ω3 OXLP and HM sEVs effectively reduced inflammation in IBD-model mice, preventing colon shortening, infiltration of inflammatory cells and tissue fibrosis.DiscussionIncorporating this unique cocktail of oxylipins into fortified milk formulas might reduce the risk of NEC in PIs and also provide immunological and neurodevelopmental support.
Prevalence of serum MOG antibody and AQP4 antibody in optic neuritis after SARS-CoV-2 infection
Authors: Sun Chuan-bin 
Published: November 2023

PurposeTo evaluate the prevalence of serum myelin oligodendrocyte glycoprotein antibody (MOG-Ab) and aquaporin-4 antibody (AQP4-Ab) in optic neuritis (ON) patients after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection by cell-based indirect immunofluorescence assay (CBA).MethodsIn this prospective case series study, 35 patients clinically diagnosed as ON and laboratory-confirmed SARS-CoV-2 infection from 8 December 2022 to 8 February 2023 were included. All patients’ clinical and laboratory data were collected and analyzed.ResultsThe mean age of the 35 patients (46 eyes) was 38.2 years (ranging from 6 to 69 years), and 17 cases were female patients. Thirty-three and two cases showed positive SARS-CoV-2 RNA test results before or shortly after ON onset, respectively. ON occurred unilaterally in 24 cases and bilaterally in 11 cases. Ophthalmic examination revealed swollen optic disc in 37 eyes, normal optic disc in 6 eyes, and temporally or wholly paled optic disc in 3 eyes. CBA revealed seropositive MOG-Ab in 10 cases and AQP4-Ab in 2 cases, respectively, of which 2 AQP4-Ab-seropositive cases and 1 MOG-Ab-seropositive case had a past medical history of ON. Most ON patients showed a rapid and dramatic response to pulse steroid therapy. The median of BCVA at the onset and at the last follow-up was 20/500 (ranging from light perception to 20/20) and 20/67 (ranging from counting fingers to 20/20), respectively.ConclusionSerum MOG-Ab and AQP4-Ab were detected in 28.6% (10/35) and 5.7% (2/35) ON cases after SARS-CoV-2 infection. SARS-CoV-2 infection may trigger an onset or a relapse of ON, as well as the production of MOG-Ab.
Effect of seaweed canopy disturbance on understory microbial communities on rocky shores
Authors: Vadillo Gonzalez Sebastian , Wood Georgina , Tiong Hui Yi Regine , Lema Kimberley A. , Mayer-Pinto Mariana , Lauro Federico M. , Kjelleberg Staffan , Bulleri Fabio , Steinberg Peter D. , Marzinelli Ezequiel M. 
Published: November 2023

IntroductionThe collapse of macroalgal habitats is altering the structure of benthic communities on rocky shores globally. Nonetheless, how the loss of canopy-forming macroalgae influences the structure of epilithic microbial communities is yet to be explored. MethodsHere, we used experimental field manipulations and 16S-rRNA-gene amplicon sequencing to determine the effects of macroalgal loss on the understory bacterial communities and their relationship with epiphytic bacteria on macroalgae. Beds of the fucoid Hormosira banksii were exposed to different levels of disturbance resulting in five treatments: (i) 100% removal of Hormosira individuals, (ii) 50% removal, (iii) no removal, (iv) a procedural control that mimicked the removal process, but no Hormosira was removed and (v) adjacent bare rock. Canopy cover, bacterial communities (epilithic and epiphytic) and benthic macroorganisms were monitored for 16 months.ResultsResults showed that reductions in canopy cover rapidly altered understory bacterial diversity and composition. Hormosira canopies in 50% and 100% removal plots showed signs of recovery over time, but understory epilithic bacterial communities remained distinct throughout the experiment in plots that experienced full Hormosira removal. Changes in bacterial communities were not related to changes in other benthic macroorganisms.DiscussionThese results demonstrate that understory epilithic bacterial communities respond rapidly to environmental disturbances at small scales and these changes can be long-lasting. A deeper knowledge of the ecological role of understory epilithic microbial communities is needed to better understand potential cascading effects of disturbances on the functioning of macroalgal-dominated systems.
Association between transcutaneous oxygen saturation within 24 h of admission and mortality in critically ill patients with non-traumatic subarachnoid hemorrhage: a retrospective analysis of the MIMIC-IV database
Authors: Liu Junjie , Zhao Zongxu , Li Jianmin , Zhang Qiuhua , Wang Yichao , Zhang Junwei 
Published: November 2023

BackgroundIn critically ill patients, transcutaneous oxygen saturation (SpO2) upon admission is typically associated with in-hospital mortality. Nevertheless, the available information for patients with non-traumatic subarachnoid hemorrhage (SAH) is limited. In our study, our objective was to assess the correlation between SpO2 levels and mortality among patients diagnosed with severe SAH.MethodsIn this study, we extracted data from the Medical Information Marketplace in Intensive Care (MIMIC-IV) database, which comprises information on critically ill patients. By employing matching ICD-9 and ICD-10 codes, we identified 3,328 patients diagnosed with SAH. Every individual who was admitted to the intensive care unit (ICU) had their SpO2 data and various covariates, including age, sex, diagnosis, and duration of stay, recorded upon admission. Subsequently, the patients were categorized into three distinct groups according to their SpO2 levels: low (≤95%), moderate (95–98%), and high (≥98%). To investigate the association between percutaneous oxygen saturation and mortality in patients with severe SAH, logistic regression, and cubic spline models were utilized. The main outcomes of interest were 28- and 90-day mortality rates. Additionally, subgroup analyses were conducted to evaluate these correlations and assess the consistency of interactions.ResultsA cohort of 864 patients diagnosed with non-traumatic SAH was included in this study. The correlation between SpO2 and mortality displayed a U-shaped curve when utilizing a finite cubic spline function (non-linearity &lt; 0.001), with the nadir in the probability of in-hospital death at 96%. Mortality at 28 and 90 days showed an inverse correlation with SpO2 &lt; 96% (adjusted odds ratio [OR], 0.8; 95% confidence interval [CI], 0.67–0.95, and 0.76; 95% CI, 0.6–0.96). Conversely, there was a positive correlation between percutaneous oxygen saturation (SpO2) levels of ≥96% and mortality rates at both 28 and 90 days (adjusted OR, 1.17; 95% CI, 1.02–1.35 and 1.2; 95% CI, 1.05–1.39).ConclusionIn patients with severe subarachnoid hemorrhage, the association between SpO2 and mortality at 28 and 90 days demonstrated a U-shaped pattern. When SpO2 levels were between 95 and 98%, both short- and long-term mortality rates were at their lowest. Patients with significant subarachnoid hemorrhage had a lower chance of survival when their SpO2 values were either high or low.
The anti-diabetic effects of metformin are mediated by regulating long non-coding RNA
Authors: Chang Wenguang , Li Wei , Li Peifeng 
Published: November 2023

Type 2 diabetes (T2D) is a metabolic disease with complex etiology and mechanisms. Long non-coding ribonucleic acid (LncRNA) is a novel class of functional long RNA molecules that regulate multiple biological functions through various mechanisms. Studies in the past decade have shown that lncRNAs may play an important role in regulating insulin resistance and the progression of T2D. As a widely used biguanide drug, metformin has been used for glucose lowering effects in clinical practice for more than 60 years. For diabetic therapy, metformin reduces glucose absorption from the intestines, lowers hepatic gluconeogenesis, reduces inflammation, and improves insulin sensitivity. However, despite being widely used as the first-line oral antidiabetic drug, its mechanism of action remains largely elusive. Currently, an increasing number of studies have demonstrated that the anti-diabetic effects of metformin were mediated by the regulation of lncRNAs. Metformin-regulated lncRNAs have been shown to participate in the inhibition of gluconeogenesis, regulation of lipid metabolism, and be anti-inflammatory. Thus, this review focuses on the mechanisms of action of metformin in regulating lncRNAs in diabetes, including pathways altered by metformin via targeting lncRNAs, and the potential targets of metformin through modulation of lncRNAs. Knowledge of the mechanisms of lncRNA modulation by metformin in diabetes will aid the development of new therapeutic drugs for T2D in the future.
The Learning Collaboratory: developing and evaluating public health students’ skills while promoting community health
Authors: Horigian Viviana E. , Perrino Tatiana , Kornfeld Julie , Schmidt Renae D. , Gonzalez Sophia T. 
Published: November 2023

IntroductionComplex and continuous developments in health and healthcare require innovative changes in programs that educate public health scientists and professionals. Public health change agents need critical competencies to confront today and tomorrow’s leading problems including leadership, communication, interprofessional practice, and systems thinking.The context: challenges in public health educationPublic Health training programs teach competencies through their applied field experience and culminating project, typically late in the program, and often implemented in isolation from peers and faculty. Objectives and skills do not always align closely with community-based program needs. Students pursuing a degree in science in public health need to deeply comprehend multi-dimensional and interconnected systemic problems and communicate with diverse stakeholders across disciplines to produce relevant community-engaged research. The University of Miami Public Health Learning Collaboratory (LC) was established to transform the learning experience of public health master’s students by providing opportunities to develop necessary core skills for effective public health practice early in their training, while applying these skills to address real-world public health needs in the community.The Learning Collaboratory: structure, pedagogical approach and programmatic detailsSpanning an average of 3 semesters, the LC promotes student involvement in collaborative and impactful capstone and thesis projects. Practice-based teaching and service learning are central approaches to teaching cross-cutting competencies of leadership, communication, problem solving, collaboration, and systems thinking in public health. Significant to the approach is the engagement of previous cohorts of senior students to teach back to junior students, further integrating concepts learned. Long term alumni feedback recognized strengths of the program, including its structure, teamwork &amp; collaboration, critical thinking &amp; problem solving, guidance, nurture &amp; support, teaching back, and content &amp; curriculum. Community partners agreed the LC prepared students to practice in the field of public health.DiscussionThe LC is a promising model for master’s level public health education and community application, given the opportunities it provides to strengthen and integrate students’ public health skills in a supportive environment, and enhance the transferability and sustainability of student and faculty’s community public health work.
Comprehensive immune profiling of SARS-CoV-2 infected kidney transplant patients
Authors: Fenninger Franz , Sherwood Karen R. , Wu Vivian , Wong Paaksum , DeMarco Mari L. , Wang Meng , Benedicto Vincent , Dwarka Krishna A. , Günther Oliver P. , Tate Logan , Yoshida Eric , Keown Paul A. , Kadatz Matthew , Lan James H. 
Published: November 2023

IntroductionThe immune responses of kidney transplant recipients against SARS-CoV-2 remains under studied.MethodsIn this prospective pilot study, we performed comprehensive immune profiling using cellular, proteomic, and serologic assays on a cohort of 9 kidney transplant recipients and 12 non-transplant individuals diagnosed with COVID-19.ResultsOur data show that in addition to having reduced SARS-CoV-2 specific antibody levels, kidney transplant recipients exhibited significant cellular differences including a decrease in naïve—but increase in effector T cells, a high number of CD28+ CD4 effector memory T cells, and increased CD8 T memory stem cells compared with non-transplant patients. Furthermore, transplant patients had lower concentrations of serum cytokine MIP-1β as well as a less diverse T cell receptor repertoire.ConclusionOverall, our results show that compared to non-transplant patients, kidney transplant recipients with SARS-CoV-2 infection exhibit an immunophenotype that is reminiscent of the immune signature observed in patients with severe COVID-19.