bluuebunny/crossref_metadata_2025_split huggingface dataset:
DesignScrum–An agility educational resource powered by creativity
Authors: Villarrubia Carlos , Vara Juan Manuel , Granada David , Gómez‐Macías Cristian , Pérez‐Blanco Francisco Javier 
Published: January 2024

AbstractAgile methods have been widely adopted by the industry and its teaching has seen a surge, particularly in the software development field. However, these methods have a number of limitations which affect product outcomes, such as the fact that many software development companies now use Scrum to get developers to work without interruption between iterations, rather than to maintain a sustainable rhythm. Agile experts have stated the importance of incorporating creativity into Scrum, and although there are several agile resources that help with the learning process, it seems essential to approach such learning from a practical point of view. Furthermore, none of these resources introduce creativity. In this paper, we present an educational resource in the form of a serious game that allows you to acquire all the key concepts of agile and creative methods. The game is based on the use of LEGO pieces to simulate a real project, applying the key concepts of the Scrum and Design Thinking frameworks in a gamified way. It was assessed in a professional training centre of computer science by using surveys through which participants evaluated their previous knowledge of agile and creativity methods. We analysed the improvement of these competences, as well as the general level of satisfaction with the game. After the game, the results showed that the participants' knowledge of the Scrum and Design Thinking frameworks had improved and that they were very satisfied with the whole experience.
A review on microplastics in major European rivers
Authors: Gao Sijia , Orlowski Natalie , Bopf Franziska Kristin , Breuer Lutz 
Published: January 2024

AbstractThe topic of riverine microplastics is of great interest to the general public, yet the univocal scientific knowledge on this topic is limited. This review investigated the occurrence of microplastics in 6 major European rivers and their tributaries based on the results from 29 studies. We examined the reviewed studies in regard to data quality and reproducibility and assessed the abundance of microplastics in different sections of the water column. Furthermore, we investigated the chemical composition and potential origin of the reported riverine microplastics. We found that polystyrene, polypropylene, and polyethylene were the most abundant polymer types. The majority of primary microplastics arose from the industry sector as well as from personal care and cleaning products, whereas secondary microplastics constituted fibers from synthetic textiles and fragments of diverse origins. We highlighted the diversity of experimental and analytical approaches that could lead to high uncertainties in the measurements of microplastics abundance. Furthermore, the presence of microplastics in rivers was found to vary spatially likely due to point and nonpoint pollution sources of anthropogenic activities. Heterogenous environmental processes impacted the fate of microplastics characterized by various forms, sizes, and densities, in different ways. This impeded the identification of representative quantitative measurements of microplastics across different time frames. We advocate for the development of standardized protocols by the research community to ensure higher reproducibility of sampling, processing, and analysis of microplastics in aquatic environments. We recommend long‐term and site‐specific monitoring on microplastics with high data comparability to better inform policy making.This article is categorized under:
Science of Water > Water Quality
Water and Life > Conservation, Management, and Awareness
Manifestly covariant worldline actions from coadjoint orbits. Part I. Generalities and vectorial descriptions
Authors: Basile Thomas , Joung Euihun , Oh TaeHwan 
Published: January 2024

Abstract
We derive manifestly covariant actions of spinning particles starting from coadjoint orbits of isometry groups, by using Hamiltonian reductions. We show that the defining conditions of a classical Lie group can be treated as Hamiltonian constraints which generate the coadjoint orbits of another, dual, Lie group. In case of (inhomogeneous) orthogonal groups, the dual groups are (centrally-extended inhomogeneous) symplectic groups. This defines a symplectic dual pair correspondence between the coadjoint orbits of the isometry group and those of the dual Lie group, whose quantum version is the reductive dual pair correspondence à la Howe. We show explicitly how various particle species arise from the classification of coadjoint orbits of Poincaré and (A)dS symmetry. In the Poincaré case, we recover the data of the Wigner classification, which includes continuous spin particles, (spinning) tachyons and null particles with vanishing momenta, besides the usual massive and massless spinning particles. In (A)dS case, our classification results are not only consistent with the pattern of the corresponding unitary irreducible representations observed in the literature, but also contain novel information. In dS, we find the presence of partially massless spinning particles, but continuous spin particles, spinning tachyons and null particles are absent. The AdS case shows the largest diversity of particle species. It has all particles species of Poincaré symmetry except for the null particle, but allows in addition various exotic entities such as one parameter extension of continuous particles and conformal particles living on the boundary of AdS. Notably, we also find a large class of particles living in “bitemporal” AdS space, including ones where mass and spin play an interchanged role. We also discuss the relative inclusion structure of the corresponding orbits.
Even-point multi-loop unitarity and its applications: exponentiation, anomalies and evanescence
Authors: Carrasco John Joseph M. , Pavao Nicolas H. 
Published: January 2024

Abstract
We identify novel structure in newly computed multi-loop amplitudes and quantum actions for even-point effective field theories, including both the nonlinear sigma model (NLSM) and double-copy gauge theories such as Born-Infeld and its supersymmetric generalizations. We exploit special properties of all even-point theories towards establishing an efficient unitarity based amplitude construction. In doing so, we find evidence that the leading IR divergence of NLSM amplitudes exponentiates when the target space is $$ \mathbbm{CP} $$
CP
1 ≅ SU(2)/U(1). We then systematically compute the two-loop anomalous behavior of Born-Infeld, and find that the counterterms needed to restore U(1) invariant behavior at loop-level can be constructed via a symmetric-structure double-copy. We also demonstrate that the divergent part of the one-minus (−+++) two-loop anomaly vanishes upon introducing an evanescent operator. In addition to these purely photonic counterterms, we verify through explicit calculation that the anomalous matrix elements that violate U(1) duality invariance can be alternatively cancelled by summing over internal $$ \mathcal{N} $$
N
= 4 DBIVA superfields. Finally we find that $$ \mathcal{N} $$
N
= 4 Dirac-Born-Infeld-Volkov-Akulov (DBIVA) amplitudes admit double-copy construction through two-loop order by reproducing our unitarity based result with a double copy between color-dual $$ \mathcal{N} $$
N
= 4 super-Yang-Mills and our two-loop NLSM amplitudes. This result supports the possibility of color-dual representations for NLSM beyond one-loop. We conclude with an overview of how D-dimensional four-photon counterterms can be constructed in generality with the symmetric-structure double-copy, and outline a convenient way of counting evanescent operators using Hilbert series as generating functions.
Pharmacological diacylglycerol lipase inhibition impairs contextual fear extinction in mice
Authors: Ramos-Medina Liorimar , Rosas-Vidal Luis E. , Patel Sachin 
Published: January 2024

AbstractAcquisition and extinction of associative fear memories are critical for guiding adaptive behavioral responses to environmental threats, and dysregulation of these processes is thought to represent important neurobehavioral substrates of trauma and stress-related disorders including posttraumatic stress disorder (PTSD). Endogenous cannabinoid (eCB) signaling has been heavily implicated in the extinction of aversive fear memories and we have recently shown that pharmacological inhibition of 2-arachidonoylglycerol (2-AG) synthesis, a major eCB regulating synaptic suppression, impairs fear extinction in an auditory cue conditioning paradigm. Despite these data, the role of 2-AG signaling in contextual fear conditioning is not well understood. Here, we show that systemic pharmacological blockade of diacylglycerol lipase, the rate-limiting enzyme catalyzing in the synthesis of 2-AG, enhances contextual fear learning and impairs within-session extinction. In sham-conditioned mice, 2-AG synthesis inhibition causes a small increase in unconditioned freezing behavior. No effects of 2-AG synthesis inhibition were noted in the Elevated Plus Maze in mice tested after fear extinction. These data provide support for 2-AG signaling in the suppression of contextual fear learning and the expression of within-session extinction of contextual fear memories.
Crawl away from the light! Assessing behavioral and physiological photoprotective mechanisms in tropical solar-powered sea slugs exposed to natural light intensities
Authors: Burgués Palau Laia , Senna Giulia , Laetz Elise M. J. 
Published: January 2024

AbstractPhotosynthesizers face a trade-off regarding light: they need enough to maintain high photosynthetic rates, yet excess leads to oxidative stress. Despite this, light and its detrimental effects are chronically underestimated. Solar-powered sea slugs (Sacoglossa: Gastropoda) provide the ideal lens with which to study this trade-off, since they steal chloroplasts from algae but do not inherit photoacclimation and photorepair capacities. We collected three slug species in Curaçao during March and December 2022, comparing the amount of light they received in nature to their optimal light intensities for photosynthesis, and their preferred light intensities. We then investigated behavioral and physiological photoprotection mechanisms to determine if and how they limit light. Finally, we examined oxidative activity under optimal and excess light. All three species were naturally exposed to more light (> 1000 µmol m−2 s−1) than is optimal or preferred. Elysia crispata (kleptoplast retention for > 3 months) is fully exposed to light in nature but reduces the light reaching its kleptoplasts via parapodial shading. Elysia velutinus retains kleptoplasts for ~ 2 weeks and hides in its macroalgal food, limiting light exposure. Both species displayed low amounts of oxidative activity under optimal light, which increased slightly under excess light. Elysia ornata retained chloroplasts for ~ 3 days, lacked observable photoprotection and always displayed high levels of oxidative activity, potentially explaining its limited capacity for kleptoplast retention. Furthermore, both E. velutinus and E. ornata display strong light-avoidance behaviors. This study clearly demonstrates links between high light intensities, photoprotection, and oxidative stress, highlighting the need for future studies that examine aquatic photosynthesizers under natural lighting.
Metabolic engineering of Shewanella oneidensis to produce glutamate and itaconic acid
Authors: Wohlers Hannah , Zentgraf Laura , van der Sande Lisa , Holtmann Dirk 
Published: January 2024

Abstract
Shewanella oneidensis is a gram-negative bacterium known for its unique respiratory capabilities, which allow it to utilize a wide range of electron acceptors, including solid substrates such as electrodes. For a future combination of chemical production and electro-fermentation, the goal of this study was to expand its product spectrum. S. oneidensis was metabolically engineered to optimize its glutamate production and to enable production of itaconic acid. By deleting the glutamate importer gltS for a reduced glutamate uptake and pckA/ptA to redirect the carbon flux towards the TCA cycle, a ∆3 mutant was created. In combination with the plasmid pG2 carrying the glutamate dehydrogenase gdhA and a specific glutamate exporter NCgl1221 A111V, a 72-fold increase in glutamate concentration compared to the wild type was achieved. Along with overexpression of gdhA and NCgl1221 A111V, the deletion of gltS and pckA/ptA as well as the deletion of all three genes (∆3) was examined for their impact on growth and lactate consumption. This showed that the redirection of the carbon flux towards the TCA cycle is possible. Furthermore, we were able to produce itaconic acid for the first time with a S. oneidensis strain. A titer of 7 mM was achieved after 48 h. This suggests that genetic optimization with an expression vector carrying a cis-aconitate decarboxylase (cadA) and a aconitate hydratase (acnB) along with the proven redirection of the carbon flux to the TCA cycle enabled the production of itaconic acid, a valuable platform chemical used in the production of a variety of products.

Key points
•Heterologous expression of gdhA and NCgl1221_A111V leads to higher glutamate production.
•Deletion of ackA/pta redirects carbon flux towards TCA cycle.
•Heterologous expression of cadA and acnB enables itaconic acid production.
Analysis of substrate specificity of cytochrome P450 monooxygenases involved in trichothecene toxin biosynthesis
Authors: Cardoza Rosa E. , McCormick Susan P. , Martínez-Reyes Natalia , Rodríguez-Fernández Joaquín , Busman Mark , Proctor Robert H. , Gutiérrez Santiago 
Published: January 2024

AbstractTrichothecenes are a structurally diverse family of toxic secondary metabolites produced by certain species of multiple fungal genera. All trichothecene analogs share a core 12,13-epoxytrichothec-9-ene (EPT) structure but differ in presence, absence and types of substituents attached to various positions of EPT. Formation of some of the structural diversity begins early in the biosynthetic pathway such that some producing species have few trichothecene biosynthetic intermediates in common. Cytochrome P450 monooxygenases (P450s) play critical roles in formation of trichothecene structural diversity. Within some species, relaxed substrate specificities of P450s allow individual orthologs of the enzymes to modify multiple trichothecene biosynthetic intermediates. It is not clear, however, whether the relaxed specificity extends to biosynthetic intermediates that are not produced by the species in which the orthologs originate. To address this knowledge gap, we used a mutant complementation-heterologous expression analysis to assess whether orthologs of three trichothecene biosynthetic P450s (TRI11, TRI13 and TRI22) from Fusarium sporotrichioides, Trichoderma arundinaceum, and Paramyrothecium roridum can modify trichothecene biosynthetic intermediates that they do not encounter in the organism in which they originated. The results indicate that TRI13 and TRI22 could not modify the intermediates that they do not normally encounter, whereas TRI11 could modify an intermediate that it does not normally encounter. These findings indicate that substrate promiscuity varies among trichothecene biosynthetic P450s. One structural feature that likely impacts the ability of the P450s to use biosynthetic intermediates as substrates is the presence and absence of an oxygen atom attached to carbon atom 3 of EPT.
A comparison of visual assessment and semi-quantification for the diagnostic and prognostic use of [18F]flortaucipir PET in a memory clinic cohort
Authors: Mathoux Gregory , Boccalini Cecilia , Peretti Debora E. , Arnone Annachiara , Ribaldi Federica , Scheffler Max , Frisoni Giovanni B. , Garibotto Valentina 
Published: January 2024

Abstract
Purpose
[18F]Flortaucipir PET is a powerful diagnostic and prognostic tool for Alzheimer’s disease (AD). Tau status definition is mainly based in the literature on semi-quantitative measures while in clinical settings visual assessment is usually preferred. We compared visual assessment with established semi-quantitative measures to classify subjects and predict the risk of cognitive decline in a memory clinic population.

Methods
We included 245 individuals from the Geneva Memory Clinic who underwent [18F]flortaucipir PET. Amyloid status was available for 207 individuals and clinical follow-up for 135. All scans were blindly evaluated by three independent raters who visually classified the scans according to Braak stages.
Standardized uptake value ratio (SUVR) values were obtained from a global meta-ROI to define tau positivity, and the Simplified Temporo-Occipital Classification (STOC) was applied to obtain semi-quantitatively tau stages. The agreement between measures was tested using Cohen’s kappa (k). ROC analysis and linear mixed-effects models were applied to test the diagnostic and prognostic values of tau status and stages obtained with the visual and semi-quantitative approaches.

Results
We found good inter-rater reliability in the visual interpretation of tau Braak stages, independently from the rater’s expertise (k>0.68, p<0.01). A good agreement was equally found between visual and SUVR-based classifications for tau status (k=0.67, p<0.01). All tau-assessment modalities significantly discriminated amyloid-positive MCI and demented subjects from others (AUC>0.80) and amyloid-positive from negative subjects (AUC>0.85). Linear mixed-effect models showed that tau-positive individuals presented a significantly faster cognitive decline than the tau-negative group (p<0.01), independently from the classification method.

Conclusion
Our results show that visual assessment is reliable for defining tau status and stages in a memory clinic population. The high inter-rater reliability, the substantial agreement, and the similar diagnostic and prognostic performance of visual rating and semi-quantitative methods demonstrate that [18F]flortaucipir PET can be robustly assessed visually in clinical practice.
Comparable quantification methodologies in archaeobotany – a work-in-progress and debate
Authors: Bates Jennifer , Jiménez-Arteaga Carolina 
Published: January 2024

AbstractThe way archaeobotanists name and quantify seed fragments is a determinant step not only in the interpretation of a given macrobotanical assemblage, but also in the degree of comparability across different sites. However, seed terminology and quantification have yet not been standardised among scholars but rely on the various geographical and laboratory traditions, as well as specific research needs and circumstances. This has created two major biases: first, the main focus has been put on plants of economic importance, specially Near Eastern and European cereals (barley, wheat, rye and oats); and second, while there has been notable discussion about quantification methods such as ubiquities, densities, proportions, or more complex multivariate statistics, there is often little explicit discussion of the actual first counting stage (i.e. how one goes from things under a microscope to things in raw data or how the Minimum Number of Individuals -MNI- is calculated). In the case of South Asian archaeobotany, the economic role of other cereal species (e.g. millets, rice) and non-cereal crops (e.g. pulses, oilseeds), as well as the usually high fragmentation state in which macrobotanical remains are found, lead us to reflect on the need to establish a more accurate and comparable quantification methodology in the region. We believe that applying this to all seed fragments will also be an important tool to better understand the role of wild taxa (e.g. fruits) in ancient diets and improve the potential contribution of weeds to disentangle past agricultural systems. In this paper, we propose a new work-in-progress terminology and counting method which, far from concluded, is intended to be a starting point for future fruitful debate and development.
Analysis of swallowing and voice-related quality of life in patients after supracricoid partial laryngectomy
Authors: Liu Tianzhen , Feng Huajun , Liang Zhuoping , Xu Shengen , Qin Gang 
Published: January 2024

Abstract
Objective
This study evaluated the swallowing and voice function of laryngeal cancer patients after Supracricoid Partial Laryngectomy(SCPL), and its influence on quality of life to provide a reference for the selection of surgical methods for laryngeal cancer patients.

Methods
Twenty-one patients who received SCPL between April 2015 and November 2021 were included. Each patient’s swallowing function and quality of life were assessed through fiberoptic endoscopic examination of swallowing (FEES) and the M.D. Anderson Dysphagia Inventory (MDADI). Fundamental, jitter, shimmer, maximum phonation time (MPT), and voice handicap index-10 (VHI-10) were performed to assess voice function and voice-related quality of life.

Results
The results of the FEES of the 21 patients were as follows: the rates of pharyngeal residue after swallowing solid, semiliquid, and liquid food were 0%, 28.57%, and 38.09%, respectively; the rates of laryngeal infiltration after swallowing solid, semiliquid, and liquid food were 0%, 28.57%, and 4.76%, respectively; and aspiration did not occur in any of the patients. In the evaluation of swallowing quality of life, the mean total MDADI score was 92.6 ± 6.32. The voice function evaluation showed that the mean F0, jitter, shimmer, and MPT values were 156.01 ± 120.87 (HZ), 11.57 ± 6.21 (%), 35.37 ± 14.16 (%) and 7.85 ± 6.08 (s), respectively. The mean total VHI-10 score was 7.14 ± 4.84.

Conclusion
SCPL provides patients with satisfactory swallowing and voice function. The patients in this study were satisfied with their quality of life in terms of swallowing and voice. SCPL can be used as a surgical method to preserve laryngeal function in patients with laryngeal cancer.
Pattern electroretinogram, blue-yellow visual evoked potentials and the risk of developing visual field defects in glaucoma suspects: a longitudinal “survival” analysis with a very long follow-up
Authors: Huchzermeyer Cord , Lämmer Robert , Mardin Christian Y. , Kruse Friedrich E. , Kremers Jan , Horn Folkert K. 
Published: January 2024

Abstract
Purpose
Estimating glaucoma suspects’ risk for visual field defects helps to avoid under- and over-treatment. In this retrospective, longitudinal cohort study with a very long follow-up, we studied whether pattern electroretinograms (PERG) amplitudes and blue-on-yellow visual evoked potential (BY-VEP) latencies can predict visual field defects.

Methods
Participants of the Erlangen Glaucoma Study were examined with PERG and BY-VEP between 9/1991 and 8/2001. Stimuli were created using an optical bench with Maxwellian view and consisted of vertical gratings (0,88 cpd) in a 32° field for both PERG and BY-VEP. Patients were treated according to clinical standards and performed standard automated perimetry (SAP) annually. Retrospectively, patients with normal SAP at baseline were selected. Primary endpoint was conversion to perimetric glaucoma. Predictive value was modeled using Kaplan–Meier analyses and a multivariate cox proportional hazards model with the continuous variables PERG amplitude, BY-VEP peak time and SAP square-root of loss variance (sLV) after stratification for Jonas classification of the optic discs.

Results
Of 412 patients (288: Jonas 0, 103: I, and 21: II; baseline age: 20–60 years), 65 converted to perimetric glaucoma during follow-up (0.5–23.3 years; median 5.5 years). Optic disc classification was a strong risk factor for conversion (log rank p < 0.0001), and patients with more advanced changes progressed earlier. In the multivariate analysis (log rank p = 0.005), only PERG amplitude remained an independent risk factor after stratification for optic disc morphology (p = 0.021), with a ~ 30% higher risk per μV amplitude decrease.

Conclusions
PERG helps to estimate glaucoma suspects’ risk for visual field defects.