bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of the antigenicity of soya-bean-based infant formulas
Authors: Heppell L. M. J. , Sissons J. W. , Pedersen H. E. 
Published: September 2005

1. The antigenicity of four soya-bean-based infant formulas (Prosobee powder, Prosobee liquid concentrate (Mead Johnson, Uxbridge, Middx), Wysoy (Wyeth, Maidenhead, Berks) and Formula S (Cow and Gate, Trowbridge, Wilts)) was measured by enzyme-linked immunosorbent assays (ELISAs) specific for glycinin and β-conglycinin. Results were compared with in vivo assessments of antigenicity using guinea-pigs, rabbits and calves.2. The levels of antigenic glycinin and β-conglycinin in Wysoy and Formula S were below the limits of detection of the ELISA. Both these proteins were detected in Prosobee powder and Prosobee liquid concentrate with the highest levels, especially for glycinin, being present in Prosobee powder.3. Wysoy was sufficiently antigenic to evoke a soya-bean-specific serum antibody response in rabbits injected with this formula emulsified in complete Freunds adjuvant. A significantly greater response was obtained when rabbits were similarly injected with Prosobee powder.4. The formulas varied in their ability to sensitize guinea-pigs for both anaphylaxis and antibody production when given orally, although the differences were not statistically significant. Prosobee powder appeared to be the most antigenic and Formula S the least, with Prosobee liquid concentrate and Wysoy being intermediate.5. Similar variations in antigenicity were observed when Prosobee powder, Wysoy and Formula S were fed to soya-bean-sensitive calves. These formulas were all capable of provoking intestinal disturbances (seen as increased ileal flow-rate, decreased small intestinal transit time and decreased nitrogen absorption) but the most severe reactions were seen when Prosobee powder was fed and the least with Formula S.6. Thus the four soya-bean-based infant formulas showed considerable differences in antigenicity. In vivo studies using guinea-pigs, rabbits and calves were in good agreement and broadly correlated with the immunochemical assessment of antigenicity. However, the in vitro and in vivo results did not correspond exactly and levels of glycinin and β-conglycinin below the limit of detection by ELISA could evoke an immune response in the different animal species. We believe that these variations in antigenicity of different commercial products prepared from isolated soya-bean protein may be important when interpreting the results from studies of the development of allergy in infants given soya-bean-based formulas.
The effect of fasting on enzyme levels in the enlarged and involuting rat pancreas
Authors: Crass R. A. , Oatesa P. S. , Morgan R. G. H. 
Published: September 2005

1. The effect on pancreatic digestive enzyme levels of fasting and changes from a diet containing trypsin inhibitor (raw soya-bean flour, RSF) to diets free of trypsin inhibitor (heated soya-bean flour, HSF, or commercial rat chow) was studied in rats for up to 7 d.2. In RSF-fed rats killed without fasting, enzyme levels were low, but after fasting for 24 h before killing there was a marked increase in all enzyme levels. Histological studies showed that pancreatic acinar cells from RSF-fed rats killed without fasting were devoid of zymogen granules, but following a 24 h fast there was a marked accumulation of zymogen granules which extend into the basal cytoplasm. Fasting either produced no change or a fall in enzyme levels in rats fasted after feeding HSF or chow continuously.3. If animals fed on RSF were changed to HSF and either fed or fasted for 24 h up to the time of killing there was an increase in amylase (EC3. 2. 1. 1), trypsin (EC3. 4. 21. 4), lipase (triacylglycerol lipase;EC3. 1. 1. 3) and protein 1 d after the change, followed by a fall over the next 6 d to levels similar to those seen in rats fed on HSF continuously.4. Animals changed from RSF to chow showed similar effects as far as trypsin, lipase and protein were concerned, but amylase rose, to reach the level seen in rats fed on chow continuously (about ten times that seen in soya-bean-fed rats), after 2 d.5. These results suggest that in the rats fed on RSF, pancreatic enzyme synthesis is rapid but secretion is equally rapid and intracellular enzyme levels are low. When these animals are fasted or changed to a diet free of trypsin inhibitor the rate of secretion falls but the high rate of synthesis continues for at least 24 h and enzymes accumulate in the pancreas. In studies of pancreatic enzyme levels in rats fed on trypsin inhibitor the extent of fasting before killing the animal is therefore an important variable. Such animals should probably not be fasted before study.
Evaluation of the net energy value of glucose (cerelose) and maize starch in diets for rainbow trout (Salmo gairdneri)
Authors: Hilton John W. , Atkinson James L. , Slinger Stanley J. 
Published: September 2005

1. Quadruplicate groups of rainbow trout (Sulmo gairdneri) (mean body-weight 24.9 g) were reared on six dietary treatments (practical-type diets) in a modified paired-feeding experiment for 12 weeks at 15° to determine the net energy (NE) value of starch and glucose to rainbow trout.2. Three test diets were prepared to contain (g/kg): 0 supplemented carbohydrate (diet 1), 250 maize starch (diet 2) and 250 glucose (diet 3) and were givenad lib.to the trout with the feeding rate of the glucose- and starchfed groups being monitored after each feeding. The remaining three treatments involved controlled feeding of the trout with diet 1 at 75% of the feed intake of trout reared on diets 2 and 3, so as to provide the same levels of protein and lipids without carbohydrate, and with diet 2 at 100% of the feed intake of trout reared on diet 3.3. The difference in the final carcass energy of thead lib.-fed group and the respective controlled-fed group divided by the amount of dietary glucose or starch energy consumed by the trout is the NE value for that carbohydrate.4. The determined NE value of glucose was 3.99 kJ/g and starch 2.17 kJ/g, which is 24.6 and 12.6% respectively of the gross energy values of these carbohydrates in rainbow trout.5. The results indicate that digestible energy and calculated metabolizable energy values for carbohydrates in rainbow trout overestimate the utilizable energy content of the diet.6. The determined NE values for glucose and starch in the present study should be used with caution since various factors (such as the feeding rate determined in the present study) may affect the utilization of dietary carbohydrates in rainbow trout.
Reduction of energy requirements of steers fed on low-quality-roughage diets using trenbolone acetate
Authors: Hunter R. A. , Vercoe J. E. 
Published: September 2005

1. Six steers implanted with 300 mg trenbolone acetate and six steers not implanted were fed on low protein, low-quality-roughage dietsad lib.in two experiments. The steers were Hereford (Bos taurus) × Brahman (Bos indicus) crossbreds (50:50), initially of about 400 kg mean live weight (LW). In the first experiment of 8 weeks duration roughage was given alone. In the second experiment of 6 weeks duration the diet was supplemented with 100 g urea and 4.6 g sulphur daily. The same steers were implanted in each experiment. At the conclusion of each experiment metabolic rate was measured after a 72 h fast.2. In the first experiment control and implanted steers had similar rates of LW loss (0.57 and 0.59 kg/d respectively). Implanted steers had significantly (P < 0.01) lower feed intakes (12.8v.10.9 g dry matter (DM)/kg LW), significantly (P < 0.01) lower fasting metabolic rates even after adjustment for intake (83.3v. 74.5 kJ/kg per d) and significantly (P < 0.01) lower plasma insulin concentrations (24v.19 μunits/ml). Differences in plasma concentrations of free 3,5,3′-triiodothyronine (T3), non-esterified fatty acids and urea-nitrogen were not significant.3. In the second experiment intake of the supplement diet was similar in both control and trenbolene acetate treated steers (19.5 and 20.0 g DM/kg LW respectively). LW gains were 0.23 and 0.41 kg/d for control and implanted steers respectively, the difference being s ignificant (P < 0.05). Fasting metabolic rate (76.9v.70.7 kJ/kg per d) was significantly (P < 0.05) lower in implanted steers.
The relation between sodium chloride concentration in drinking water and egg-shell damage
Authors: Balnave Derick , Yoselewitz Israel 
Published: September 2005

1.A significant linear increase in egg-shell defects from 60-week-old laying hens, and corresponding significant linear decreases in various egg-shell-quality measurements, were observed in response to increasing concentrations of sodium chloride in the drinking water, to the maximum concentration of 600 mg/l used in the present study.2. The incidence of damaged egg shells was increased 3-fold by including NaCl in the drinking water at a concentration of 600 mg/l.3. Shell defects declined when birds were placed on normal water for 5 weeks but were still 1.4- to 2.1-fold greater than control values.4. After an induced rest from lay on normal water, shell defects were still 1.3- to 3.2-fold greater in birds which had previously received the NaCl in the drinking water.5. The increased incidence of shell damage was not related to decreased food intake or increased egg weight or production.
Nutritional encephalomalacia in the chick: an exposure of the vulnerable period for cerebellar development and the possible need for both ω6- and ω3-fatty acids
Authors: Budowski P. , Leighfield M. J. , Crawford M. A. 
Published: September 2005

1. Cockerels (1-d-old) received over a period of 4 weeks, a balanced diet containing either safflower oil (diet S) or linseed oil (diet L) as a source of polyunsaturated fatty acids (PUFA). Body-weight, and weights of cerebrum and cerebellum increased at similar rates in the two dietary groups. The total fatty acids (FA) of the cerebellum differed from the cerebral FA by their higher PUFA and oleic acid contents and their lower stearic acid level. During the 3rd week of life there was a spurt in accretion of PUFA in the cerebellum, but not in the cerebrum. At the end of the experimental period phosphatidylethanolamine was present at twice the concentration in the cerebellum, compared with the cerebrum.2. Diets S and L resulted in extensive mutual replacement of ω6- and ω3-FA in brain, without any significant change in the total PUFA. Brain oleic acid concentration was higher in the diet-L group than in the diet-S group, but saturated FA were not affected by the dietary treatments.3. These results may be relevant to basic brain biology and to chick nutritional encephalomalacia (NE). This disease, which specifically affects the cerebellum and is readily induced by diets supplying linoleic acid but deficient in vitamin E, usually reaches its highest incidence during the 3rd week of life and may thus be related to the cerebellar PUFA spurt that occurs at that time. The fact that NE was induced by linoleic acid, while α-linolenic acid exerted a protective action, points to an overproduction of arachidonic-derived eicosanoids as a factor in the etiology of the cerebellar lesion and possibly a structural change due to a loss of docosahexaenoic acid and gain of arachidonic acid in the chicks given diet S.
Histidine requirement of kittens for growth, haematopoiesis and prevention of cataracts
Authors: Quam Darcy D. , Morris James G. , Rogers Quinton R. 
Published: September 2005

1. The histidine requirement of growing kittens was determined from an experiment in which forty-eight kittens were randomly allocated to six amino acid-based diets supplying: 1.0, 1.5, 2.0, 2.5, 3.0 or 4.5 g histidine base/kg diet.2. By 48 d it was obvious that 1.0 and 1.5 g histidine/kg diet were grossly inadequate so the kittens receiving these two diets were removed from the experiment. The other four groups of kittens continued to receive their diets for a total of 128 d.3. Mean daily weight gain, nitrogen retention and food intake attained plateau values at 2.1 g histidine/kg diet.4. Blood samples taken at 25 and 48 d after kittens were given the diets showed a significant effect of dietary histidine on haemoglobin (Hb) concentration. Hb and packed cell volume (PCV) attained asymptotic values at 3.0 g histidine/kg diet at 48 d. At 128 d, kittens consuming diets containing 2.0–4.5 g histidine/kg had similar Hb and PCV values.5. Cataracts of both eyes were observed in two of nine female kittens which had received diets containing either 2.0 or 2.5 g histidine/kg.6. A concentration of 3 g histidine/kg diet is recommended as a practical guide for feeding kittens.7. There was a rectilinear relation (r20.99) between the logarithm of the histidine concentration of plasma and the concentrationof histidine in the diet over the range 1.5–3.0 g histidine/kg diet.
Adaptations in protein metabolism during lactation in the rat
Authors: Naismith Donald J. , Robinson Siân M. 
Published: September 2005

1. The activities of two hepatic enzymes that participate in the regulation of amino acid oxidation and urea synthesis were measured in lactating rats (day 15 of lactation) and virgin controls. The enzymes were alanine aminotransferase (EC2. 6. 1.2) and argininosuccinate synthase (EC6. 3. 4.5). Carcasses of the dams were also analysed.2. Changes in the activities of both enzymes in dams fedad lib. on a diet containing an excess of protein indicated that amino acid oxidation was depressed. In dams restricted in protein to the level of intake of their controls but allowed to satisfy their needs for energy, enzyme activities were significantly reduced. In these animals lean tissue catabolism supplemented the dietary protein supply.3. This adjustment in protein metabolism which effectively spares protein for milk-protein synthesis could be explained either by a reduction in the availability of substrate in the liver, or by the intervention of an anabolic hormone secreted in lactation.
Vitamin A reserve of liver in health and coronary heart disease among ethnic groups in Singapore
Authors: Saha N. , Ng T. B. , Tan P. Y. , Wee K. P. 
Published: September 2005

1. The vitamin A content of human liver tissue was determined in 363 autopsy samples. The sample comprised a total of 181 subjects dying after accidents and 182 dying from coronary heart disease among Singapore ethnic groups of both sexes.2. The medium vitamin A reserve was 146 mg/kg in accident victims and 141 mg/kg in those who had died of coronary heart disease. Of all the samples 16% contained less than 40 mg/kg, 45% had 100–300 mg/kg, while 9% contained more than 500 mg/kg liver.3. Among the accident victims, Indians had the lowest median liver vitamin A reserve (118 mg/kg) compared with that in other ethnic groups (137 mg/kg in Chinese, 191 mg/kg in Malays, 155 mg/kg in Caucasians).4. The ethnic distribution of vitamin A reserve in coronary deaths was similar to that in accident victims.5. There was no significant difference between the sexes in hepatic vitamin A reserve.6. The distribution of vitamin A reserve in all the groups was skewed to the right.
Trace nutrients
Authors: Lewis Janet , Buss David H. 
Published: September 2005

1. The amounts of magnesium, copper, zinc, phosphorus, manganese, potassium, vitamin B6, vitamin B12, folate, pantothenic acid, biotin, vitamin E and dietary fibre in the British household diet were calculated by applying appropriate values from recent analytical studies to the amounts of foods recorded in the National Food Survey during 1986.2. National average intakes were (mg/person per d): Mg 247, Cu 1.25, Zn 9.0, P 1249, Mn 3.43, K 2694, vitamin B61.73, vitamin B126.33μg, folate 230μg, pantothenic acid 6.07, biotin 35μg, vitamin E 8.4. Regional and income-group differences were estimated, and found to be small. Additional contributions from alcoholic drinks and confectionery were also determined.3. Dietary fibre was estimated both as unavailable carbohydrate and as non-starch polysaccharide. The national average intakes were 21.8 and 12.9 g/d respectively.4. Intakes were compared with Canadian (Department of National Health and Welfare, 1983) and American (National Research Council, 1980) recommended dietary allowances (RDAs). With the exception of biotin, the Canadian RDAs were met by the household diet but the much higher American RDAs were only met for vitamin B12and pantothenic acid.
Food intake and work allocation of male and female farmers in an impoverished Indian village
Authors: Edmundson W. C. , Edmundson S. A. 
Published: September 2005

1. The energy balance and activity allocation of eight male and eight female Indian farmers were meaured for 4 d during 10 months in both the dry and wet seasons of 1983.2. All food prepared and eaten was weighed and nutrient values were calculated from food composition tables.3. Average energy costs per unit mass for fifteen tasks were estimated from data obtained using a Kofranyi-Michaelis respirometer. Each subject was observed from 05.00 to 20.00 hours and activities were recorded for each minute. The remaining activity was determined by recall.4. Activity allocation was categorized qualitatively into time spent on economically productive work, free time and body maintenance.5. The mean weight for male subjects was 48.2 kg; the average female weight was only 36.9 kg. The mean height for male and female subjects was 1.61 m and 1.49 m respectively.6. For male subjects, the daily mean energy intake of 9.81 MJ (2350 kcal) was close to the calculated energy output of 9.54 MJ (2285 kcal).7. The women's calculated daily expenditure of 8.22 MJ (1968 kcal) was slightly higher than their mean energy intake of 7.73 MJ (1852 kcal).8. Although intake and output varied in relation to sex, weight and wealth the mean intake per kg body mass was equal for both males and females at 210 kJ (50 kcal) per kg. This is close to the minimum requirement for active Third World farmers.9. The women spent significantly more time on economically productive work activities than the men (11.1 v. 8.1 h/d). Although female energy intake was 27 % less than males, their productive work time was 27% higher.
Nitrogen balance study in young Nigerian adult males using four levels of protein intake
Authors: Atinmo T. , Mbofung C. M. F. , Egun G. , Osotimehin B. 
Published: September 2005

1. The present study was carried out to estimate precisely, via the nitrogen balance technique, the protein requirement of Nigerians (earlier estimated via the obligatory N method) using graded levels of protein intake.2. Fifteen medical students of the University of Ibadan who volunteered to participate in the study were given graded levels of protein (0.3, 0.45, 0.6 and 0.75 g/kg body-weight per d) derived from foods similar to those usually consumed by the subjects.3. Each subject was given each of the dietary protein levels for a period of 10 d. Subjects were divided into two groups and the feeding pattern followed a criss-cross design with one group starting with the highest level of protein intake (0.75 g) and the second group starting with the lowest level of protein intake (0.3 g). Mean energy intake during each of the eleven experimental periods was maintained at 0.2 MJ/kg per d. After an initial 5d adaptation period in each experimental period, 24 h urine and faecal samples were collected in marked containers for five consecutive days for N determination.4. Mean N balance during consumption of the four protein levels (0.30, 0.45, 0.6 and 0.75 g/kg) were −11.02 (SD 8.07), −9.90 (SD 6.64), + 9.70 (SD 4.15) and + 5.13 (SD 4.62) respectively. Using regression analysis, the mean daily N requirement was estimated at 110.25 mg N/kg body-weight (0.69 g protein/kg body-weight). Estimates of allowances for individual variations to cover 97.5% of the population adjusted this value to 0.75 g protein/kg body-weight. Net protein utilization for the diet at maintenance level was estimated at 57.5.
Growth, ascorbic acid and iron contents of tissues of young guinea-pigs whose dams received high or low levels of dietary ascorbic acid or Fe during pregnancy and suckling
Authors: Bates C. J. , Cowen T. D. , Tsuchiya Harumi 
Published: September 2005

1. Guinea-pig dams were fed on purified diets containing high (5 g/kg diet plus 1 g/l drinking water) or moderate (0.5 g/kg diet) levels of ascorbic acid, in combination with high (1 g/kg diet) or moderate (0.043 g/kg diet) levels of iron, during pregnancy and suckling. Their offsprings' diets contained 0.1 g ascorbic acid/kg and 0.04 g Fe/kg.2. High ascorbic acid intake clearly enhanced both tissue ascorbate and Fe storage in the dams, and high Fe intake increased both the dams' and the pups' tissue Fe stores.3. In the animals receiving high Fe intake, a co-existing high ascorbate intake by the dams reduced the growth rate of the offspring, but only during the early stages of development, not during the later stages of post-weaning growth. All the pups' tissue ascorbate levels fell after weaning, but those born of the dams receiving the high ascorbic acid diets did not fall to levels lower than those of the other pups.4. Thus, although certain disadvantages to the offspring resulting from very-high ascorbic acid intake by pregnant guinea-pig dams were detected, these did not include permanently increased ascorbate requirements, and hence a progression to scurvy as the pups grew and matured.
Influence of low- and high-protein diets on glucose homeostasis in the rat
Authors: Okitolonda W. , Brichard S. M. , Pottier A. M. , Henquin J. C. 
Published: September 2005

1. The influence of the protein content of the diet on glucose homeostasis was studied in the rat. Rats of 28 d of age received ad lib. a control diet containing (g/kg) 150 protein (P15), or a diet containing 50 protein (P5) or 450 protein (P45). Since P5 rats spontaneously reduced their food intake, a fourth group of rats (P25) received the same amount of energy as P5 rats and the same amount of protein as P15 rats.2. After 12–13 weeks on these diets, plasma glucose and insulin levels were similar in fed P45, P25 and control P15 rats, but were lower in P5 rats. In fasted animals, plasma glucose and insulin levels were also decreased in P5 rats, whereas plasma glucose levels were increased in both P45 and P25 animals.3. During an oral glucose tolerance test, the glucose rise was only slightly larger in P5 than in P15 rats in spite of a considerably smaller increase in insulin levels. P45 rats displayed a normal tolerance to glucose with a normal insulin response, whereas tolerance to glucose was slightly poorer in P25 rats in spite of a normal insulin response.4. Pancreatic insulin stores were lower in P5 than in control P15 rats, not only because of the smaller size of their pancreas, but also because of a decrease in the insulin concentration in the gland. A much smaller decrease was also observed in P25 rats, whereas insulin reserves were not altered in P45 rats.5. It is concluded that the changes in glucose homeostasis observed in protein-energy malnutrition (P5 rats) are due to protein deprivation rather than to energy deprivation. A high-protein diet has little influence on glucose homeostasis in the rat.