bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide survey reveals the genetic background of Xinjiang Brown cattle in China
Authors: Wang Xiao , Ma Zhen , Gao Liang , Yuan Lixin , Ye Zhibing , Cui Fanrong , Guo Xiaoping , Liu Wujun , Yan Xiangmin 
Published: January 2024

Introduction: Xinjiang Brown cattle are a famous dual-purpose (dairy-beef) cultivated breed in China that occupy a pivotal position within the cattle breeding industry in Xinjiang, China. However, little information is available on the genetic background of this breed. To fill this research gap, we conducted a whole-genome screen using specific-locus amplified fragment sequencing to examine the genetic structure and diversity of 130 Xinjiang Brown cattle-grazing type (XBG, traditional type) cattle.Methods: A subsequent joint analysis incorporating two ancestral breeds, specifically 19 Brown Swiss (BS) foreign and nine Kazakh (KZ) Chinese cattle, as well as 20 Xinjiang Brown cattle-housing type (XBH) cattle, was used to explore the genetic background of the Xinjiang Brown cattle.Results: The results showed that, after nearly a century of crossbreeding, XBG cattle formed a single population with a stable genetic performance. The genetic structure, genetic diversity, and selection signature analysis of the two ancestral types showed highly different results compared to that of XBH cattle. Local ancestry inference showed that the average proportions of XGB cattle within the BS and KZ cattle lineages were 37.22% and 62.78%, respectively, whereas the average proportions of XBH cattle within the BS and KZ cattle lineages were 95.14% and 4.86%, respectively. Thus, XGB cattle are more representative of all Xinjiang Brown cattle, in line with their breeding history, which involves crossbreeding. Two complementary approaches, fixation index and mean nucleotide diversity, were used to detect selection signals in the four aforementioned cattle breeds. Finally, the analysis of 26 candidate genes in Xinjiang Brown cattle revealed significant enrichment in 19 Gene Ontology terms, and seven candidate genes were enriched in three pathways related to disease resistance (CDH4, SIRPB1, and SIRPα) and the endocrine system (ADCY5, ABCC8, KCNJ11, and KCNMA1). Finally, development of the core SNPs in XBG cattle yielded 8,379 loci.Conclusion: The results of this study detail the evolutionary process of crossbreeding in Xinjiang Brown cattle and provide guidance for selecting and breeding new strains of this species.
Characterization of CD4+ and CD8+ T cells responses in the mixed lymphocyte reaction by flow cytometry and single cell RNA sequencing
Authors: Mangelinck Adèle , Dubuisson Agathe , Becht Etienne , Dromaint-Catesson Sandra , Fasquel Manon , Provost Nicolas , Walas Dawid , Darville Hélène , Galizzi Jean-Pierre , Lefebvre Céline , Blanc Véronique , Lombardi Vincent 
Published: January 2024

BackgroundThe Mixed Lymphocyte Reaction (MLR) consists in the allogeneic co-culture of monocytes derived dendritic cells (MoDCs) with T cells from another donor. This in vitro assay is largely used for the assessment of immunotherapy compounds. Nevertheless, the phenotypic changes associated with lymphocyte responsiveness under MLR have never been thoroughly evaluated.MethodsHere, we used multiplex cytokine and chemokine assays, multiparametric flow cytometry and single cell RNA sequencing to deeply characterize T cells activation and function in the context of CD4+- and CD8+-specific MLR kinetics.ResultsWe showed that CD4+ and CD8+ T cells in MLR share common classical markers of response such as polyfunctionality, increased proliferation and CD25 expression but differ in their kinetics and amplitude of activation as well as their patterns of cytokines secretion and immune checkpoints expression. The analysis of immunoreactive Ki-67+CD25+ T cells identified PBK, LRR1 and MYO1G as new potential markers of MLR response. Using cell-cell communication network inference and pathway analysis on single cell RNA sequencing data, we also highlighted key components of the immunological synapse occurring between T cells and the stimulatory MoDCs together with downstream signaling pathways involved in CD4+ and CD8+ T cells activation.ConclusionThese results provide a deep understanding of the kinetics of the MLR assay for CD4+ or CD8+ T cells and may allow to better characterize compounds impacting MLR and eventually identify new strategies for immunotherapy in cancer.
Higher levels of systemic immune-inflammatory index are associated with the prevalence of gallstones in people under 50 years of age in the United States: a cross-sectional analysis based on NHANES
Authors: Meng Chunli , Liu Kai 
Published: January 2024

IntroductionInflammation plays a significant role in gallstones formation. The prognosis of various illnesses was initially predicted using the systemic immune-inflammatory index (SII). We performed an updated analysis on the impact of SII and gallstones.MethodsTo investigate the connection between the SII and gallstones occurrence in a sample of individuals from the National Health and Nutrition Examination Survey (NHANES) database from 2017 to 2020, we employed logistic regression analysis, subgroup analysis, and smoothing curve fitting.ResultsIn our study, an aggregate of 4,950 individuals over the age of 20 were enrolled, and 429 of them claimed to have gallstones. A fully adjusted model showed that the third and fourth quartiles of SII was parallel associated with gallstones in adults (OR = 2.43, 95% CI = 1.39–4.26; OR = 2.97, 95% CI = 1.72–5.16) under 50 years. Subgroup analysis and smoothed curve fitting provided evidence in favor of this finding.ConclusionAccording to our research, gallstones are more likely to occur in US adults younger than 50 years.
Exercise for reducing chemotherapy-induced peripheral neuropathy: a systematic review and meta-analysis of randomized controlled trials
Authors: Huang Yingjie , Tan Tian , Liu Lu , Yan Zijian , Deng Yuexia , Li Guangyao , Li Min , Xiong Jia 
Published: January 2024

BackgroundMore than half of cancer patients develop severe chemotherapy-induced peripheral neuropathy (CIPN), resulting in low quality of life, negative effects on function, and challenges in treatment compliance. Most recent studies have shown that exercise therapy has a positive impact on reducing CIPN symptoms and can also improve quality of life, balance, and activity levels. The aim of this meta-analysis was to evaluate the effect of exercise therapy on the efficacy of CIPN.MethodsComputerized search of Embase, Web of Science, CNKI, Wan Fang Data, VIP, CBM for RCTs on exercise therapy for CIPN from database creation to November 2022, without language restriction. The Cochrane Handbook 5.3 risk of bias assessment tool was used to evaluate the quality of the included studies. Then Revman 5.3 software was used to evaluate the quality of the included studies. The heterogeneity of the research results is tested by I2, continuous variables were presented as weighted mean difference or standard mean difference, and confidence intervals were set at 95%. Stata15.0 was utilized to conduct a meta-analysis.ResultsA total of 15 RCTs with 1,124 patients were included. Meta-analysis showed that the test group was superior to the control group in terms of total symptom score (SMD: −0.62; 95% Cl: −0.99, −0.24), numbness, tingling, quality of life score (total score, physical, function), pain, balance, and neurotoxicity function assessment (FACT/GOG-NTX) questionnaire (p &lt; 0.05).LimitationsThe duration and frequency of treatment are different every week, which may have some impact on the results.ConclusionExercise therapy can be effective in treating CIPN by improving symptom score (total symptom score, numbness, tingling), quality of life score (total score, physical function), pain, balance, and FACT/GOG-NTX questionnaires. It still needs to be refined and validated by more high-quality, multicenter, large-sample RCTs in the future.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022373131, identifier: CRD42022373131.
The economic impact of cannabis use disorder and dementia diagnosis in veterans diagnosed with traumatic brain injury
Authors: Esmaeili Aryan , Pogoda Terri K. , Amuan Megan E. , Garcia Carla , Del Negro Ariana , Myers Maddy , Pugh Mary Jo , Cifu David , Dismuke-Greer Clara 
Published: January 2024

BackgroundStudies have demonstrated that individuals diagnosed with traumatic brain injury (TBI) frequently use medical and recreational cannabis to treat persistent symptoms of TBI, such as chronic pain and sleep disturbances, which can lead to cannabis use disorder (CUD). We aimed to determine the Veterans Health Administration (VHA) healthcare utilization and costs associated with CUD and dementia diagnosis in veterans with TBI.MethodsThis observational study used administrative datasets from the population of post-9/11 veterans from the Long-term Impact of Military-Relevant Brain Injury Consortium-Chronic Effects of Neurotrauma Consortium and the VA Data Warehouse. We compared the differential VHA costs among the following cohorts of veterans: (1) No dementia diagnosis and No CUD group, (2) Dementia diagnosis only (Dementia only), (3) CUD only, and (4) comorbid dementia diagnosis and CUD (Dementia and CUD). Generalized estimating equations and negative binomial regression models were used to estimate total annual costs (inflation-adjusted) and the incidence rate of healthcare utilization, respectively, by dementia diagnosis and CUD status.ResultsData from 387,770 veterans with TBI (88.4% men; median [interquartile range (IQR)] age at the time of TBI: 30 [14] years; 63.5% white) were followed from 2000 to 2020. Overall, we observed a trend of gradually increasing healthcare costs 5 years after TBI onset. Interestingly, in this cohort of veterans within 5 years of TBI, we observed substantial healthcare costs in the Dementia only group (peak = $46,808) that were not observed in the CUD and dementia group. Relative to those without either condition, the annual total VHA costs were $3,368 higher in the CUD only group, while no significant differences were observed in the Dementia only and Dementia and CUD groups.DiscussionThe findings suggest that those in the Dementia only group might be getting their healthcare needs met more quickly and within 5 years of TBI diagnosis, whereas veterans in the Dementia and CUD group are not receiving early care, resulting in higher long-term healthcare costs. Further investigations should examine what impact the timing of dementia and CUD diagnoses have on specific categories of inpatient and outpatient care in VA and community care facilities.
Neuroprotective effect of ranolazine improves behavioral discrepancies in a rat model of scopolamine-induced dementia
Authors: Samir Shereen M. , Hassan Hend M. , Elmowafy Rasha , ElNashar Eman Mohamed , Alghamdi Mansour Abdullah , AlSheikh Mona Hmoud , Al-Zahrani Norah Saeed , Alasiri Faten Mohammed , Elhadidy Mona G. 
Published: January 2024

BackgroundRanolazine (Rn), an antianginal agent, acts in the central nervous system and has been used as a potential treatment agent for pain and epileptic disorders. Alzheimer’s disease (AD) is one of the most prevalent neurodegenerative diseases and the leading factor in dementia in the elderly.AimWe examined the impact of Rn on scopolamine (Sco)-induced dementia in rats.MethodsThirty-two albino male rats were divided into four groups: control, Rn, Sco, and Rn + Sco.ResultsA significant decrease in the escape latency in the Morris water maze test after pre-treatment with Rn explained better learning and memory in rats. Additionally, Rn significantly upregulated the activities of the antioxidant enzymes in the treated group compared to the Sco group but substantially reduced acetylcholinesterase activity levels in the hippocampus. Moreover, Rn dramatically reduced interleukin-1 β (IL-1β) and IL-6 and upregulated the gene expression of brain-derived neurotrophic factor (BDNF). Furthermore, in the Sco group, the hippocampal tissue’s immunohistochemical reaction of Tau and glial factor activating protein (GFAP) was significantly increased in addition to the upregulation of the Caspase-3 gene expression, which was markedly improved by pre-treatment with Rn. The majority of pyramidal neurons had large vesicular nuclei with prominent nucleoli and appeared to be more or less normal, reflecting the all-beneficial effects of Rn when the hippocampal tissue was examined under a microscope.ConclusionOur findings indicated that Rn, through its antioxidative, anti-inflammatory, and anti-apoptotic effects, as well as the control of the expression of GFAP, BDNF, and Tau proteins, has a novel neuroprotective impact against scopolamine-induced dementia in rats.
Therapeutic efficacy of sulforaphane in autism spectrum disorders and its association with gut microbiota: animal model and human longitudinal studies
Authors: Yang Jiexian , He Li , Dai Si , Zheng Huihui , Cui Xilong , Ou Jianjun , Zhang Xiaojie 
Published: January 2024

IntroductionSulforaphane (SFN) has been found to alleviate complications linked with several diseases by regulating gut microbiota (GM), while the effect of GM on SFN for autism spectrum disorders (ASD) has not been studied. Therefore, this study aimed to investigate the relationship between the effects of SFN on childhood ASD and GM through animal model and human studies.MethodsWe evaluated the therapeutic effects of SFN on maternal immune activation (MIA) induced ASD-like rat model and pediatric autism patients using three-chamber social test and OSU Autism Rating Scale-DSM-IV (OARS-4), respectively, with parallel GM analysis using 16SrRNA sequencing.ResultsSFN significantly improved the sniffing times of ASD-like rats in the three-chamber test. For human participants, the average verbal or non-verbal communication (OSU-CO) scores of SFN group had changed significantly at the 12-wk endpoint. SFN was safe and no serious side effects after taking. GM changes were similar for both ASD-like rats and ASD patients, such as consistent changes in order Bacillales, family Staphylococcaceae and genus Staphylococcus. Although the gut microbiota composition was significantly altered in SFN-treated ASD-like rats, the alteration of GM was not evident in ASD patients after 12 weeks of SFN treatment. However, in the network analysis, we found 25 taxa correlated with rats' social behavior, 8 of which were associated with SFN treatment in ASD-like rats, For ASD patients, we found 35 GM abundance alterations correlated with improvements in ASD symptoms after SFN treatment. Moreover, family Pasteurellaceae and genus Haemophilus were found to be associated with SFN administration in the network analyses in both ASD-like rats and ASD patients.DiscussionThese findings suggest that SFN could provide a novel avenue for preventing and treating ASD, and its therapeutic effects might be related to gut microbiota.
Enhancing performance: unveiling the physiological impact of submaximal and supramaximal tests on mixed martial arts athletes in the −61 kg and −66 kg weight divisions
Authors: Gonçalves Aleksandro Ferreira , Miarka Bianca , Maurício Clóvis de Albuquerque , Teixeira Rafael Pereira Azevedo , Brito Ciro José , Ignácio Valenzuela Pérez Diego , Slimani Maamer , Znazen Hela , Bragazzi Nicola Luigi , Reis Victor Machado 
Published: January 2024

This study delves into the intricate details of Mixed Martial Arts (MMA) by examining key variables such as maximal oxygen uptake (VO2 peak), aerobic energy (EAER), anaerobic energy (EAN), and accumulated O2 deficit (DOA). By investigating associations and comparing athletes in the −61 kg bantamweight and −66 kg featherweight weight divisions, we aim to shed light on their physiological characteristics. The sample consisted of 20 male volunteers separated into two paired groups: ten athletes in the category up to 61 kg (age: 27.7 ± 5.9 years old, height: 170.9 ± 3.4 cm, body mass: 72.8 ± 1.4 kg, fat percentage: 9.5% ± 3.0%, professional experience: 7.5 ± 7.1 years) and ten athletes up to 66 kg (age: 27.6 ± 2.9 years old, height: 176.0 ± 5.5 cm, body mass: 77.0 ± 1.5 kg, fat percentage: 7.85% ± 0.3%, professional experience: 5.5 ± 1.5 years). Remarkably, our findings revealed striking similarities between the two weight divisions. Furthermore, we discovered a negative correlation between VO2 peak and the number of MMA fights, indicating a potential impact of professional experience on aerobic capacity (r = −0.65, p &lt; 0.01). Additionally, the number of fights exhibited negative correlations with anaerobic energy (r = −0.53, p &lt; 0.05) and total energy cost (r = −0.54, p &lt; 0.05). These results provide valuable insights for designing training programs in the context of MMA. While training both weight divisions together can be beneficial, it is equally crucial to incorporate specific weight-class-focused training to address each division’s unique physical demands and requirements.
Automatic classification of ligneous leaf diseases via hierarchical vision transformer and transfer learning
Authors: Han Dianyuan , Guo Chunhua 
Published: January 2024

BackgroundIdentification of leaf diseases plays an important role in the growing process of different types of plants. Current studies focusing on the detection and categorization of leaf diseases have achieved promising outcomes. However, there is still a need to enhance the performance of leaf disease categorization for practical applications within the field of Precision Agriculture.MethodsTo bridge this gap, this study presents a novel approach to classifying leaf diseases in ligneous plants by offering an improved vision transformer model. The proposed approach involves utilizing a multi-head attention module to effectively capture contextual information about the images and their classes. In addition, the multi-layer perceptron module has also been employed. To train the proposed deep model, a public dataset of leaf disease is exploited, which consists of 22 distinct kinds of images depicting ligneous leaf diseases. Furthermore, the strategy of transfer learning is employed to decrease the training duration of the proposed model.ResultsThe experimental findings indicate that the presented approach for classifying ligneous leaf diseases can achieve an accuracy of 85.0% above.DiscussionIn summary, the proposed methodology has the potential to serve as a beneficial algorithm for automated detection of leaf diseases in ligneous plants.
Development and psychometric validation of the hospitalized patients’ expectations for treatment scale-clinician version
Authors: Dai Bindong , Xiao Chunfeng , Wang Yufei , Li Tao , Duan Yanping , Jiang Yinan , Shi Lili , Hong Xia , Geng Wenqi , Hu Jiaojiao , Cao Jinya , Wei Jing 
Published: January 2024

ObjectivePatient safety management systems in general hospitals require a comprehensive tool for assessing the expectations of inpatients across different wards. This study aimed to develop and psychometrically validate a new scale, the hospitalized patients’ expectations for treatment scale-clinician version (HOPE-C), to meet this requirement.MethodsWe interviewed 35 experts and 10 inpatients while developing the HOPE-C scale. The scale was initially designed with three dimensions: clinicians’ expectations regarding doctor-patient communication, clinicians’ expectations regarding treatment outcome, and clinicians’ expectations regarding disease management. We recruited 200 inpatients from a general hospital in China. At the same time, 51 clinicians were assigned to the enrolled patients who completed the HOPE-C to examine the reliability, validity, and psychometric characteristics of the questionnaire. We applied item analysis, assessed construct validity, evaluated internal consistency, and conducted a test-retest reliability analysis over 7 days.ResultsBoth exploratory and confirmatory analyses supported a 2-dimensional structure, comprising doctor-patient communication expectations and treatment outcome expectations, with favorable model fit parameters (root mean square residual [RMR] = 0.042, root mean square error of approximation [RMSEA] = 0.049, comparative fit index [CFI] = 0.989, Tucker-Lewis index [TLI] = 0.984). Item analysis demonstrated appropriate item design (r = 0.744–0.961). The scale exhibited strong internal consistency, with Cronbach’s α values of 0.884, 0.816, and 0.840 for the overall scale, the doctor-patient communication expectation subscale, and the treatment outcome expectation subscale, respectively. The 7-day test-retest reliability was 0.996 (p &lt; 0.001).ConclusionOur findings suggest that the HOPE-C is a reliable and valid assessment tool for measuring the expectations of inpatients in general hospitals. It effectively identifies patients’ expectations concerning doctor-patient communication and treatment outcomes.
A latent class analysis of community-based rehabilitation needs among Chinese older adults: a mixed study protocol
Authors: Xu Lei , Xue Caixiu , Yang Ke , Chen Lingyun , Chen Xidong , Xie Xiaohui , Wang Jia , Wang Xueting , Wang Lianhong 
Published: January 2024

BackgroundGeriatric diseases (e.g., chronic diseases and geriatric syndromes) may result in impaired physical performance and a decline in the quality of life. The results of previous studies reported the positive effects of comprehensive community-based rehabilitation (CBR) services on physical and social functioning and psychosocial wellbeing. However, to provide adequate and personalised rehabilitation services, it is essential to understand the needs of the older adults population. There have been no studies on the need for CBR in older adults populations that consider their heterogeneity. Therefore, high-quality studies are required to recognise the heterogeneity and latent classes of CBR needs in older adults population groups. This study aims to identify the heterogeneity of the rehabilitation needs of older adults in the community and explore whether older adults with similar characteristics have similar needs through a cross-sectional survey and latent class analysis (LCA) to provide support for personalised rehabilitation services.MethodsThe study is structured into four phases. The first phase will focus on constructing a comprehensive questionnaire to assess rehabilitation needs. In the second phase, a pilot study will be conducted to evaluate the reliability and validity of the completed questionnaire. This step ensures the robustness of the instrument for data collection. The third phase will involve cross-sectional surveys using the finalised questionnaires to collect the necessary data from the targeted population. The fourth phase will focus on conducting LCA to determine the CBR needs of the older adult population.DiscussionThe results of this study will provide novel and critical information for a better understanding of the rehabilitation needs, potential categories, and influencing factors of older adults in the community. The study will be conducted in Guizhou Province in western China, where economic and social development is relatively low, and the results will inform and benefit other regions and developing countries facing similar challenges. However, because of the complete social security and rehabilitation service systems in developed areas, our research results may not fully reflect the situation in these areas. Future studies may need to be conducted in places with different levels of social development.Clinical trial registrationhttps://www.chictr.org.cn/showproj.html?proj=191398, ChiCTR2300071478.