bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of polymorphic tetranucleotide microsatellite markers for the brown anole (Anolis sagrei)
Authors: Bardeleben Carolyne , Palchevskiy Vyacheslav , Calsbeek Ryan , Wayne Robert K. 
Published: May 2004

AbstractEleven polymorphic microsatellite markers have been developed for the brown anole,Anolis sagrei. The number of alleles range from five to 14 per locus with the observed heterozygosity ranging from 0.46 to 0.92. These markers will be useful for analysis of questions concerning population genetic structure and reproductive behaviour.
Simple‐sequence repeat (SSR) markers of Japanese plum (Prunus salicina Lindl.) are highly polymorphic and transferable to peach and almond
Authors: Mnejja M. , Garcia‐Mas J. , Howad W. , Badenes M. L. , Arús P. 
Published: May 2004

AbstractThirty‐five polymorphic microsatellites were developed using a CT/AG enriched genomic library of Japanese plum cv. Santa Rosa. Twenty‐seven of them detected a single locus and eight two or more loci. A high level of variability was observed in a set of eight cultivars for the 27 single‐locus microsatellites: 5.7 average number of alleles per locus; 73% mean observed heterozygosity and 74% discrimination power. Most SSRs were transferable to peach (85%) and almond (78%).
Polymorphic tetranucleotide microsatellite loci in the Hwamei (Garrulax canorus canorus) (Timaliidae)
Authors: Huang Yi‐Jiun , Chen Chienhsun , Li Shou‐Hsien 
Published: May 2004

AbstractEight polymorphic tetrarepeat (GATA)n, microsatellite loci were isolated from a babbler, Hwamei (Garrulax canorus canorus). We evaluated the polymorphism of these microsatellite loci by genotyping 36–48 individuals from the Asian mainland. The number of alleles for each locus ranged from eight to 29. The heterozygosity was between 0.587 and 0.978. Except for one locus, genotype frequencies of these microsatellites did not significantly deviate from the Hardy–Weinberg expectation. These markers should be useful for monitoring potential hybridization between different Hwamei subspecies and provide new insights into the mating system and geographical differentiation of these birds.
Isolation and characterization of microsatellite loci in the invasive Alliaria petiolata (Brassicaceae)
Authors: Durka W. , Bossdorf O. , Gautschi B. 
Published: May 2004

AbstractEight novel polymorphic microsatellite loci are presented for garlic mustard (Alliaria petiolata, Brassicaceae) a European herb that is a serious invader of North American deciduous forests. The microsatellites will be useful tools to analyse pathways of introduction of garlic mustard, as well as its evolutionary potential in the invasive range.
Trinucleotide microsatellite loci for the peppered moth (Biston betularia)
Authors: Daly D. , Waltham K. , Mulley J. , Watts P. C. , Rosin A. , Kemp S. J. , Saccheri I. J. 
Published: May 2004

AbstractFourteen polymorphic trinucleotide microsatellites were isolated in the peppered moth, Biston betularia, using an enrichment protocol. Moderate to high allelic diversity (three to 22 alleles per locus) was found in a sample from northern England. We are currently using these markers to study the population structure of B. betularia in northern England, where a cline for melanism still exists.
Single copy nuclear DNA markers characterized for comparative phylogeography in Australian wet tropics rainforest skinks
Authors: Dolman G. , Phillips B. 
Published: May 2004

AbstractIn order to investigate population history and demography in skinks endemic to the wet tropics of Australia, multiple nuclear DNA markers were sought. The utility of 72 primers (including 63 published intron‐spanning ‘CATS’ primers) was tested. Seven loci were characterized and shown to be single copy by single‐strand conformation polymorphism analysis. Primers to five nuclear loci were developed, four with utility in skinks and three with utility in frogs. These observations extend the available information on intron‐spanning primers for amphibians and reptiles.
Characterization of microsatellite loci in Amphitmetus transversus (Kolbe, 1897) (Coleoptera, Curculionidae)
Authors: Patt A. , Misof B. , Wagner T. H. , Naumann C. M. 
Published: May 2004

AbstractWe developed six microsatellite markers for Amphitmetus transversus (Kolbe, 1897) (Coleoptera, Curculionidae), a beetle widely distributed in the guineo‐congolian rainforests of east Africa. The numbers of alleles per locus range from three to eight, with a mean number of 4.6. They will be used for population genetic analysis. The ongoing study concentrates on the genetic differentiation and variability of isolated populations in the Kakamega Forest, a highly degraded and fragmented rainforest remnant in western Kenya.
phorest: a web‐based tool for comparative analyses of expressed sequence tag data
Authors: Ahren Dag , Troein Carl , Johansson Tomas , Tunlid Anders 
Published: May 2004

AbstractComparative analysis of expressed sequence tags is becoming an important tool in molecular ecology for comparing gene expression in organisms grown in certain environments. Additionally, expressed sequence tag database information can be used for the construction of DNA microarrays and for the detection of single nucleotide polymorphisms. For such applications, we present phorest, a web‐based tool for managing, analysing and comparing various collections of expressed sequence tags. It is written in PHP (PHP: Hypertext Preprocessor) and runs on UNIX, Microsoft Windows and Macintosh (Mac OS X) platforms.
Characterization of microsatellite loci in red wood ants Formica (s. str.) spp. and the related genus Polyergus
Authors: Hasegawa Eisuke , Imai Shuichiro 
Published: May 2004

AbstractAnts are interesting subjects for studies of evolution of altruism. We developed 13 microsatellite loci in a red wood ant Formica (s. str.) yessensis from random amplified polymorphic DNA fragments to study genetic structure within populations and colonies. Five loci bore two to five alleles in both F. (s. str.) yessensis and F. (s. str.) truncorum and two were also polymorphic in a related species, Polyergus samurai. Results suggest that the loci will be useful in evolutionary studies on Formica and Polyergus species.
Characterization of microsatellite markers in a threatened species, the White Sands pupfish (Cyprinodon tularosa)
Authors: Iyengar A. , Stockwell C. A. , Layfield D. , Morin P. A. 
Published: May 2004

AbstractWe report the characterization of nine new microsatellite markers for a threatened species, the White Sands pupfish (Cyprinodon tularosa), using an enriched library method. These markers show moderate levels of variation (two to five alleles per locus) in the two native populations of this species and reveal substantial divergence between these two populations as indicated by a high percentage of private alleles. These markers will prove very useful in the conservation management of this rare species.
api‐calc 1.0: a computer program for calculating the average probability of identity allowing for substructure, inbreeding and the presence of close relatives
Authors: Ayres Karen L. , Overall Andrew D. J. 
Published: May 2004

AbstractIndividual identification via DNA profiling is important in molecular ecology, particularly in the case of noninvasive sampling. A key quantity in determining the number of loci required is the probability of identity (PIave), the probability of observing two copies of any profile in the population. Previously this has been calculated assuming no inbreeding or population structure. Here we introduce formulae that account for these factors, whilst also accounting for relatedness structure in the population. These formulae are implemented in api‐calc 1.0, which calculates PIave for either a specified value, or a range of values, for FIS and FST.
Isolation and characterization of microsatellite loci from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)
Authors: Scott K. D. , Lange C. L. , Scott L. J. , Graham G. C. 
Published: May 2004

AbstractFive microsatellite loci are presented for Helicoverpa armigera. These microsatellite loci were obtained through the construction of enriched libraries, overcoming previously reported difficulties with obtaining microsatellites from H. armigera and other Lepidoptera due to the low frequency of microsatellites in their genomes. The description of a further five microsatellite loci for H. armigera makes microsatellite based population genetics studies feasible.
Development of coral and zooxanthella‐specific microsatellites in three species of Pocillopora (Cnidaria, Scleractinia) from French Polynesia
Authors: Magalon Hélène , Samadi Sarah , Richard Murielle , Adjeroud Mehdi , Veuille Michel 
Published: May 2004

AbstractSince the building of coral reefs results from the association of corals and zooxanthellae, their intracellular algal symbionts, genetic markers for both organisms are essential for studying the contribution of their respective dispersal to the resilience of endangered reef ecosystems. Very few microsatellites have been obtained in corals thus far. Here we report the successful cloning of six polymorphic microsatellites (allele number: 5–15) from Pocillopora verrucosa, P. meandrina and P. damicornis. Four of them amplified coral, and two amplified zooxanthella DNA.
Isolation and characterization of microsatellite loci in Geum urbanum (Rosaceae) and their transferability within the genus Geum
Authors: Arens P. , Durka W. , Wernke‐Lenting J. H. , Smulders M. J. M. 
Published: May 2004

AbstractThirteen novel polymorphic microsatellite loci are presented for Geum urbanum (Rosaceae). The microsatellites will be useful tools to analyse the influence of landscape structure and land‐use intensity in agricultural landscapes on genetic diversity within and among populations of Geum urbanum. Transferability was tested in 19 other Geum species and two Waldsteinia species. In most species polymerase chain reaction (PCR) products of the expected range were obtained, therefore the markers reported here appear to be applicable across the whole genus.
Characterization of five microsatellite loci in the Pine Processionary Moth Thaumetopoea pityocampa (Lepidoptera Notodontidae Thaumetopoeinae)
Authors: Rousselet J. , Magnoux E. , Kerdelhué C. 
Published: May 2004

AbstractFive microsatellite markers were developed for the lepidopteran species Thaumetopoea pityocampa using an enrichment protocol. All loci could be amplified with no evidence of null alleles and will be useful for population genetic studies. The number of alleles ranged from three to 12 for a population of 30 individuals. Observed heterozygosities ranged from 0.53 to 0.80. No significant heterozygote deficiency was detected. Four markers might be of interest for Th. wilkinsoni.
Development and characterization of microsatellite markers for the fungus Ceratocystis fimbriata
Authors: Steimel J. , Engelbrecht C. J. B. , Harrington T. C. 
Published: May 2004

Abstract Ceratocystis fimbriata is a serious fungal pathogen on a wide range of plants, but many cryptic species within C. fimbriata are apparently host‐specialized. Anchor polymerase chain reaction (PCR) and simple sequence repeat (SSR) enriched libraries were used to develop 16 microsatellite markers for C. fimbriata. All markers were polymorphic when tested against isolates from four host‐specialized lineages of the pathogen. These markers will be valuable for phylogenetic and population genetic studies, as well as for tracking accidental introductions of host‐specialized forms of the pathogen.
Isolation of microsatellites in a parthenogenetic lizard Menetia greyii (Scincidae) and their utility in sexual species of the Menetia greyii complex
Authors: Gardner M. G. , Ottewell K. , Adams M. 
Published: May 2004

AbstractTetra and tri‐nucleotide microsatellite loci were isolated from a parthenogenetic form of the Australian lizard Menetia greyii, using an enrichment method with polymerase chain reaction (PCR)‐based colony screening. Primers for 23 loci were designed and 11 of these loci amplified well in a panel of 10 parthenogenetic individuals from a single population. Seven loci were further characterized and six successfully amplified and were polymorphic in all five sexual species of the Menetia greyii complex they were tested in. These loci will be used to investigate the parental ancestry and clonal diversity of various parthenogenetic forms within the Menetia greyii species complex.
Isolation and characterization of 20 microsatellite loci for an important tropical tree Shorea leprosula (Dipterocarpaceae) and their applicability to S. parvifolia
Authors: Lee S. L. , Tani N. , Ng K. K. S. , Tsumura Y. 
Published: May 2004

Abstract Shorea leprosula is an important timber tree in Southeast Asia. From a genomic library enriched for dinucleotide (CT) repeats, 20 polymorphic microsatellite markers were developed for this species. Polymorphism of these loci was evaluated in a sample of 24 adult individuals from a natural population. The number of alleles ranged from five to 15, and the observed heterozygosity ranged from 0.333 to 0.875. Probability of paternity exclusion, in the case where the mother is known, ranged from 0.285 to 0.784. These markers were also characterized for applicability in S. parvifolia; 16 loci were successfully cross‐amplified and showed high levels of polymorphism.
Polymorphic microsatellite loci from the red urchin, Strongylocentrotus franciscanus, with comments on heterozygote deficit
Authors: Mccartney Michael A. , Brayer Kathryn , Levitan Don R. 
Published: May 2004

Abstract Strongylocentrotus sea urchins are common subjects for studies in developmental and cell biology, reproductive biology, ecology, and evolution. We report 14 microsatellite loci from the red urchin, S. franciscanus, isolated for the purpose of estimating paternal success of males in experimental group spawns. Most of these loci were found to be highly polymorphic in a population from British Columbia. A high frequency of null alleles appears responsible for heterozygote deficit at a majority of these loci, but if used with appropriate caution, these microsatellites should be effective markers for studies of Strongylocentrotus populations.
Characterization of nine microsatellite loci in Cynoglossum officinale (Boraginaceae)
Authors: Korbecka G. , Wolff K. 
Published: May 2004

Abstract Cynoglossum officinale is a biennial plant pollinated by bumblebees. We developed microsatelllite loci in order to study the population genetic structure and effects of inbreeding in this species. In this paper, we describe nine polymorphic microsatellites for C. officinale. Between two and four alleles per locus were observed in a sample of 20 individuals from one population. Multiplexing allowed the seven most useful loci to be genotyped using three polymerase chain reaction (PCR) reactions.
Characterization of microsatellite loci in two primary parasitoids of the butterflyMelitaea cinxia,Cotesia melitaearumandHyposoter horticola(Hymenoptera)
Authors: Kankare Maaria , Jensen M. Kathleene , Kester Karen M. , Saccheri Ilik J. 
Published: May 2004

AbstractFive polymorphic microsatellite loci were isolated fromCotesia melitaearum(Wilkinson) and three fromHyposoter horticola(Gravenhost). Both of these parasitic Hymenoptera use the butterflyMelitaea cinxia(Linnaeus) exclusively as their host in the Åland islands in SW Finland. Allelic diversity and heterozygosity were quantified in samples from the Åland islands and other localities around Europe. In addition, microsatellite loci forC. melitaearumwere tested in five otherCotesiaspecies:C. acuminata(Reinhard),C. bignellii(Marshall),C. congregata(Say),C. cynthiae(Nixon) andC. koebelei(Riley) from several different host species in Europe and Asia.
Eight highly polymorphic microsatellite markers for the army ant Eciton burchellii
Authors: Denny A. Jay , Franks Nigel R. , Edwards Keith J. 
Published: May 2004

AbstractThe army ant Eciton burchellii is a nomadic predator in the rain forests of Central and South America. Detailed work has documented many aspects of this species’ ecology, behaviour and life history. However, a detailed investigation into within colony relatedness structure requires the development of genetic tools. Here we present eight microsatellite markers with between nine and 25 alleles. For each loci there is close agreement between observed and expected heterozygosity.
Microsatellite loci from the five‐lined skink (Eumeces fasciatus)
Authors: Howes B. J. , Lee‐Yaw J. A. , Robertson A. V. , Bogart J. P. , Lougheed S. C. 
Published: May 2004

AbstractThe geographical range of the five‐lined skink is the most expansive of any eastern North American lizard, ranging from previously glaciated southern Canada southward to the Gulf of Mexico and from the Atlantic seaboard west to Texas and Minnesota. The most northerly populations occur in southern Ontario and are of conservation concern. We developed six polymorphic dinucleotide microsatellite loci to examine the genetic structure within and among Ontario's populations and to examine the intrapopulation levels of genetic diversity relative to more southern populations.
Polymorphic microsatellite markers for inferring diversity in wild and domesticated sugar beet (Beta vulgaris)
Authors: Richards Christopher M. , Brownson Mary , Mitchell Sharon E. , Kresovich Steve , Panella Lee 
Published: May 2004

AbstractEight microsatellite loci were characterized within two cultivated beet (Beta vulgaris ssp. vulgaris) accessions and one accession of the wild progenitor of domesticated sugar beet, Beta vulgaris ssp. maritima. Allele diversity was high, yielding two to 11 alleles per locus. Polymorphism information content (PIC) values obtained for these eight loci where also high and indicate the highly informative nature of the microsatellites presented here. These described markers add to a small set of publicly available microsatellite markers for beet and will be instrumental in identifying patterns of genetic diversity and origins of domestication.
Microsatellite markers for woolly monkeys (Lagothrix lagotricha) and their amplification in other New World primates (Primates: Platyrrhini)
Authors: Di Fiore Anthony , Fleischer Robert C. 
Published: May 2004

AbstractSeven polymorphic microsatellite loci were identified for woolly monkeys (Lagothrix lagotricha) from an ‘enriched’ genomic library. For a wild population of 66 animals, these markers averaged over 10 alleles per locus and provided a combined probability for excluding a random individual from parentage of over 98%. These loci were screened in up to 13 other genera of New World monkeys, and many were variable in multiple taxa. Few other platyrrhine‐specific microsatellite markers have been identified; thus, these loci should prove valuable for studying the population genetic structure and mating system not just of Lagothrix but also of other neotropical primates.
Isolation of polymorphic microsatellite loci in three phantom midge species of the genus Chaoborus (Diptera: Chaoboridae)
Authors: Berendonk T. U. , Evans T. 
Published: May 2004

AbstractBecause of its widespread distribution in lakes and ponds Chaoborus is of great interest to many freshwater ecologists. Interestingly some species are restricted to small fish‐less water bodies, whereas other species live mostly in large lakes. To eventually test the genetic and evolutionary implications of these different lifestyles we identified microsatellite loci in three species in this preliminary study: C. obscuripes, C. crystallinus and C. flavicans. Using a biotin/streptavidin capture technique of repetitive sequences in a 96 well format, we obtained microsatellite‐enriched genomic libraries for all three species and identified six polymorphic microsatellite markers for each species.
Nuclear microsatellite markers for the date palm (Phoenix dactyliferaL.): characterization and utility across the genusPhoenixand in other palm genera
Authors: Billotte N. , Marseillac N. , Brottier P. , Noyer J.‐L. , Jacquemoud‐Collet J.‐P. , Moreau C. , Couvreur T. , Chevallier M.‐H. , Pintaud J.‐C. , Risterucci A.‐M. 
Published: May 2004

AbstractA (GA)nmicrosatellite‐enriched library was constructed and 16 nuclear simple sequence repeat (SSR) loci were characterized inPhoenix dactylifera. Across‐taxa amplification and genotyping tests showed the utility of most SSR markers in 11 otherPhoenixspecies and the transferability of some of them inElaeis guineensis, 11 species ofPritchardia,Pritchardiopsis jeanneneyiand six species ofAstrocaryum. The first to be published forP. dactylifera, these new SSR resources are available for cultivar identification, pedigree analysis, germplasm diversity as well as genetic mapping studies.
A new set of mono‐ and dinucleotide chloroplast microsatellites in Fagaceae
Authors: Sebastiani F. , Carnevale S. , Vendramin G. G. 
Published: May 2004

AbstractA new set of 14 chloroplast microsatellites, represented by mono‐ and dinucleotide repeats, was optimized in the three main species of the Fagaceae (Castanea sativa, Fagus sylvatica and Quercus petraea). The intraspecific variation was tested in some natural populations. The polymorphic microsatellites displayed two or three variants. Conservation of the primer pairs was checked on an additional set of six species of the Fagaceae and on Fraxinus excelsior. All the primer pairs produced a fragment of the expected size in the Fagaceae species while no amplification was obtained with 36% of the primers in F. excelsior.
Universal primers for the amplification of chloroplast microsatellites in grasses (Poaceae)
Authors: Provan Jim , Biss Pamela M. , McMeel Darragh , Mathews Sarah 
Published: May 2004

AbstractAttempts to design truly universal primers to amplify chloroplast microsatellites have met with limited success due to nonconservation of repeat loci across widely divergent taxa. We have used the complete chloroplast genome sequences of rice, maize and wheat to design five pairs of primers that amplify homologous mononucleotide repeats across the Poaceae (grasses). Sequencing confirmed conservation of repeat motifs across subfamilies and a preliminary study in Anthoxanthum odoratum revealed polymorphism at two loci with a haplotype diversity value of 0.495. These primers provide a valuable tool to study cytoplasmic diversity in this extensively studied and economically important range of taxa.
Isolation of 21 new polymorphic microsatellite loci in the phytopathogenic fungus Venturia inaequalis
Authors: Guérin Fabien , Franck Pierre , Loiseau Anne , Devaux Martine , Le Cam Bruno 
Published: May 2004

AbstractTwenty‐one new polymorphic microsatellite markers were isolated in the phytopathogenic fungus Venturia inaequalis, the causal agent of apple scab. An enrichment protocol was used to isolate microsatellite loci and the level of polymorphism was assessed on 44 European isolates. All loci were polymorphic with an average of 9.1 alleles per locus (range 2–24). Tests of cross‐species amplifications suggest that at least some of these microsatellites could be used in different species, mainly Spilocaea pyracanthae and S. eriobotryae.
Isolation and characterization of microsatellite markers in two basidiomycete species: Pleurotus eryngii and P. ferulae
Authors: Della Rosa V. , Cappuccio I. , Fanelli C. , Urbanelli S. 
Published: May 2004

AbstractSix polymorphic microsatellite loci were developed for the closed basidiomycete species Pleurotus eryngii and P. ferulae using an enriched genomic library. The number of alleles per locus varied between two and eight and the expected heterozygosity ranged from 0.30 to 0.72 in P. eryngii and from 0.20 to 0.73 in P. ferulae. This enrichment technique is useful in those species where microsatellite loci are rare in the genome.