bluuebunny/crossref_metadata_2025_split huggingface dataset:
The development and characterization of a CRISPR/Cas9-mediated PD-1 functional knockout rat as a tool to study idiosyncratic drug reactions
Authors: Cho Tiffany , Wierk Antonia , Gertsenstein Marina , Rodgers Christopher E , Uetrecht Jack , Henderson Jeffrey T 
Published: January 2024

Abstract
Idiosyncratic drug reactions are rare but serious adverse drug reactions unrelated to the known therapeutic properties of the drug and manifest in only a small percentage of the treated population. Animal models play an important role in advancing mechanistic studies examining idiosyncratic drug reactions. However, to be useful, they must possess similarities to those seen clinically. Although mice currently represent the dominant mammalian genetic model, rats are advantageous in many areas of pharmacologic study where their physiology can be examined in greater detail and is more akin to that seen in humans. In the area of immunology, this includes autoimmune responses and susceptibility to diabetes, in which rats more accurately mimic disease states in humans compared with mice. For example, oral nevirapine treatment can induce an immune-mediated skin rash in humans and rats, but not in mice due to the absence of the sulfotransferase required to form reactive metabolites of nevirapine within the skin. Using CRISPR-mediated gene editing, we developed a modified line of transgenic rats in which a segment of IgG-like ectodomain containing the core PD-1 interaction motif containing the native ligand and therapeutic antibody domain in exon 2 was deleted. Removal of this region critical for mediating PD-1/PD-L1 interactions resulted in animals with an increased immune response resulting in liver injury when treated with amodiaquine.
My Catastrophizing and Your Catastrophizing
Authors: Stragapede Elisa , Huber Jonathan D. , Corsini-Munt Serena 
Published: January 2024

Objectives:
Endometriosis, a chronic gynecologic pain condition, contributes to disruptions in multiple areas of life for both those affected and their partner. Pain catastrophizing has been associated with worse pain outcomes and quality of life for women with endometriosis, and with more cognitive load for partners. Examining both partners’ pain catastrophizing dyadically with our variables of interest will enhance our understanding of its associations to the distressing nature of experiencing and responding to pain during sex for couples with endometriosis.

Methods:
Persons with endometriosis experiencing pain during sex and their partners (n=52 couples; 104 individuals) completed online, self-report measures of pain catastrophizing, depressive symptoms, sexual satisfaction, and partner responses to pain. Persons with endometriosis reported on pain during sexual activity. Analyses were guided by the Actor-Partner Interdependence Model.

Results:
Persons with endometriosis’ pain catastrophizing was associated with their higher pain intensity and unpleasantness during sex. When persons with endometriosis reported more pain catastrophizing, they were less sexually satisfied and reported their partners responded more negatively to their pain. When partners reported higher catastrophizing, they were more depressed and responded more negatively to the pain.

Discussion:
Consistent with the Communal Coping Model of pain catastrophizing, although meant to elicit support from the environment, the often-deleterious cognitive process of magnifying, ruminating and feeling helpless about one’s pain (or one’s partner’s pain) is associated with poorer outcomes for the individual with pain and their romantic partner. Implications for pain management include the relevance of involving the partner and attending to pain cognitions of both members of the couple.
Impact of Preloading Strategy With Ticagrelor on Periprocedural Myocardial Injury in Patients With Non-ST Elevation Myocardial Infarction Undergoing Early Invasive Strategy
Authors: Karaca Orhan Furkan , Cimci Murat , Raimoglou Damla , Durmaz Eser , Yalman Hakan , Tekin Alpin Mert , Incesu Gunduz , Ozkan Ferit Ulas , Yavuz Betul , Karadag Bilgehan 
Published: January 2024

Abstract:
Pretreatment with an oral P2Y12 receptor blocker (before coronary angiography) versus treatment in the catheterization laboratory has been a matter of debate in patients presenting with non-ST segment elevation myocardial infarction (NSTEMI). The primary aim of this study was to assess the impact of an immediate preloading strategy with ticagrelor on periprocedural myocardial injury in patients with NSTEMI treated with an early invasive strategy. NSTEMI patients who underwent coronary angiography and subsequent percutaneous coronary intervention (PCI) within 24 hours after hospital admission were divided into 2 groups: the first group (pretreatment group) included patients who received ticagrelor pretreatment as soon as possible after admission and the second group (no pretreatment group) included patients who received a loading dose of ticagrelor after coronary angiography. The pretreatment group included 232 patients, and the no pretreatment group included 87 patients. Male patients represented the majority of the patients. The 2 groups were similar in baseline characteristics, except for a greater incidence of hypertension (P = 0.014) and higher hemoglobin levels (P = 0.01) in the pretreatment group in comparison with the no pretreatment group. Patients in the ticagrelor pretreatment group had less myocardial injury until coronary angiography based on troponin measurements collected at 12 hours after admission (P = 0.025). Patients in the ticagrelor pretreatment group also had fewer periprocedural myocardial injuries based on troponin measurements taken between 12 and 24 hours after the PCI (P = 0.026 and P = 0.022, respectively). Our findings suggested that ticagrelor pretreatment reduces periprocedural myocardial injury in NSTEMI patients who underwent PCI within 24 hours after admission.
High dietary salt intake attenuates nitric oxide mediated endothelium-dependent vasodilation and increases oxidative stress in pregnancy
Authors: Vulin Martina , Muller Andrijana , Drenjančević Ines , Šušnjara Petar , Mihaljević Zrinka , Stupin Ana 
Published: January 2024

Objective:
This study aimed to investigate the impact of dietary salt intake during normal pregnancy on maternal microvascular and macrovascular endothelium-dependent reactivity and oxidative stress level.

Materials and methods:
In this cross-sectional study, based on their 24-h urinary sodium excretion, pregnant women (37–40 weeks of gestation) were divided into three groups: normal salt (<5.75 g/day, N = 12), high salt (5.75–10.25 g/day, N = 36), and very high salt (VHS;>10.25 g/day, N = 17). Forearm skin microvascular reactivity in response to vascular occlusion, local heating (LTH) and iontophoresis of acetylcholine (AChID), as well as brachial artery flow mediated dilation (FMD) were measured. Serum nitric oxide, endocan, 8-iso-prostaglandin F2α (8-iso-PGF2α), thiobarbituric acid reactive substances (TBARS), and ferric-reducing ability of plasma assay were measured as biomarkers of endothelial function/activation and oxidative stress.

Results:
Brachial artery FMD, microvascular AChID, and LTH were significantly decreased in VHS compared with NS group, while LTH was also decreased in normal salt compared with high salt group. Nitric oxide was significantly decreased in both high salt and VHS groups compared with normal salt. Endocan, 8-iso-PGF2α, and TBARS were significantly increased in VHS compared with the normal salt group.

Conclusion:
High dietary salt intake is associated with decreased nitric oxide mediated endothelium-dependent vasodilation in peripheral microcirculation and macrocirculation of healthy pregnant women due to increased oxidative stress.
Prediction of preeclampsia from retinal fundus images via deep learning in singleton pregnancies: a prospective cohort study
Authors: Zhou Tianfan , Gu Shengyi , Shao Feixue , Li Ping , Wu Yuelin , Xiong Jianhao , Wang Bin , Zhou Chenchen , Gao Peng , Hua Xiaolin 
Published: January 2024

Introduction:
Early prediction of preeclampsia (PE) is of universal importance in controlling the disease process. Our study aimed to assess the feasibility of using retinal fundus images to predict preeclampsia via deep learning in singleton pregnancies.

Methods:
This prospective cohort study was conducted at Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine. Eligible participants included singleton pregnancies who presented for prenatal visits before 14 weeks of gestation from September 1, 2020, to February 1, 2022. Retinal fundus images were obtained using a nonmydriatic digital retinal camera during their initial prenatal visit upon admission before 20 weeks of gestation. In addition, we generated fundus scores, which indicated the predictive value of hypertension, using a hypertension detection model. To evaluate the predictive value of the retinal fundus image-based deep learning algorithm for preeclampsia, we conducted stratified analyses and measured the area under the curve (AUC), sensitivity, and specificity. We then conducted sensitivity analyses for validation.

Results:
Our study analyzed a total of 1138 women, 92 pregnancies developed into hypertension disorders of pregnancy (HDP), including 26 cases of gestational hypertension and 66 cases of preeclampsia. The adjusted odds ratio (aOR) of the fundus scores was 2.582 (95% CI, 1.883–3.616; P < 0.001). Otherwise, in the categories of prepregnancy BMI less than 28.0 and at least 28.0, the aORs were 3.073 (95%CI, 2.265–4.244; P < 0.001) and 5.866 (95% CI, 3.292–11.531; P < 0.001). In the categories of maternal age less than 35.0 and at least 35.0, the aORs were 2.845 (95% CI, 1.854–4.463; P < 0.001) and 2.884 (95% CI, 1.794–4.942; P < 0.001). The AUC of the fundus score combined with risk factors was 0.883 (sensitivity, 0.722; specificity, 0.934; 95% CI, 0.834–0.932) for predicting preeclampsia.

Conclusion:
Our study demonstrates that the use of deep learning algorithm-based retinal fundus images offers promising predictive value for the early detection of preeclampsia.
Pediatric Cryptococcosis
Authors: Gifford Alison , Jayawardena Naamal , Carlesse Fabianne , Lizarazo Jairo , McMullan Brendan , Groll Andreas H. , Warris Adilia 
Published: January 2024

Background:
Seroprevalence studies have shown that 70% of children are exposed to Cryptococcus, the most common cause of meningitis in people living with human immunodeficiency virus (HIV), but reported pediatric disease prevalence is much lower than in adults.

Methods:
PubMed and Ovid Global Health databases were searched with the terms “cryptococcosis,” “cryptococcal meningitis,” “Cryptococcus neoformans” or “Cryptococcus gattii.” All studies reporting pediatric specific data in the English language from 1980 up until December 2022 were included.

Results:
One hundred sixty-eight publications were reviewed totaling 1469 children, with the majority reported from Africa (54.2%). Sixty-five percent (961) were HIV positive, 10% (147) were non-HIV immunocompromised and 19% (281) were immunocompetent. Clinical signs and symptoms were only reported for 458 children, with fever (64%), headache (55%) and vomiting (39%) being the most common. Most children (80%) suffered from meningoencephalitis. Lung involvement was rarely described in HIV-positive children (1%), but significantly more common in the non-HIV immunocompromised (36%) and immunocompetent (40%) groups (P < 0.0001). Only 22% received the recommended antifungal combination therapy, which was significantly higher in immunocompetent children than those with HIV (39% vs. 6.8%; P < 0.0001). Overall mortality was 23%. A significant higher mortality was observed in children with HIV compared with immunocompetent children (32% vs. 16%; P < 0.001), but not compared with children with non-HIV immunosuppression (25).

Conclusions:
This is the largest review of pediatric cryptococcosis with new observations on differences in clinical presentation and outcome depending on the underlying condition. The lack of granular clinical data urges prospective clinical epidemiological studies for improved insight in the epidemiology, management and outcome of cryptococcosis in children.
Comparison of COVID-19 and Non–COVID-19 Patients in Intensive Care Unit for Secondary Infections
Authors: Oz Yasemin , Karakoc Ebru , Kara Serap , Kasifoglu Nilgun , Us Tercan , Yelken Birgul , Bilgin Muzaffer , Durmaz Gul 
Published: January 2024

Background
Bacterial secondary infections were commonly reported in viral respiratory tract infections. However, it is not clear whether there is a difference in the infection rates during the hospitalization in intensive care unit (ICU) caused by COVID-19. The aim of this study was to evaluate the secondary infections in long-term hospitalized patients in the ICU due to COVID-19 and whether COVID-19 increases the tendency to secondary infection in ICU patients.

Methods
This is a retrospective case-control study. It was conducted in a tertiary care hospital including 34-bed COVID-19 ICU and 20-bed non–COVID-19 ICU. Adult patients in the ICU hospitalized for ≥10 days were included the study. The results of microbiological cultures of blood, urine, and respiratory samples were evaluated in terms of infections during their hospitalization.

Results
The study group consisted of 51 patients with COVID-19 and the control group consisted of 57 non–COVID-19 patients. There was no significant difference between 2 groups in terms of distribution and frequency of agents isolated from blood culture. The frequency of isolation of Enterobacterales members in urine cultures and nonfermenting bacteria in respiratory samples were significantly higher in non–COVID-19 patients compared with COVID-19 patients (P < 0.05).

Conclusions
This study revealed that there was no difference among the patients with and without COVID-19 in ICU for the secondary infections when the ICU stay was prolonged. We think that prolonged hospitalization, urinary catheterization, and mechanical ventilation are more important risk factors for secondary infections in ICUs and COVID-19 does not occur an additional risk factor in prolonged ICU stay.
Effect of 3-Dimensional, Virtual Reality Models for Surgical Planning of Robotic Partial Nephrectomy on Surgical Outcomes: Qualitative Data from a Randomized Clinical Trial
Authors: Shirk Joseph D. , Thiel David D. , Wallen Eric M. , Linehan Jennifer M. , White Wesley M. , Badani Ketan K. , Porter James R. 
Published: January 2024

Purpose:
Planning for robotic-assisted partial nephrectomy requires surgeons to review two-dimensional CT or MRI scans to understand three-dimensional (3D) anatomy. We sought to determine the drivers of improvements in key surgical outcomes when using patient-specific 3D virtual reality (VR) models for operative planning.

Materials and Methods:
A multicenter, randomized, single-blind clinical trial was conducted from January 2019 to December 2020. Patients undergoing robotic-assisted partial nephrectomy were randomly assigned to a control group undergoing usual preoperative planning with CT and/or MRI only or to an intervention group where imaging was supplemented with a 3D VR model. A survey was administered to surgeons after review of the model and after the operation. The primary outcome measure was operative time, and secondary outcomes included clamp time, estimated blood loss, and hospital stay.

Results:
Qualitative data analysis of 44 cases in the treatment arm was performed. Surgeons reported seeing anatomic details much better in the 3D model 55% of the time and modified their preoperative plan based on anatomy shown in the 3D model 30% of the time. Cases in which the surgeon reported modifying their preoperative plan after viewing the model (30%) had significantly lower operative time, blood loss, and clamp time (P < .05). In addition, increased surgeon confidence after viewing the model significantly lowered operative time, estimated blood loss, and clamp time (P < .05).

Conclusions:
We demonstrate that surgeons who use 3D VR models for robotic kidney surgery are able to visualize anatomic structures and select the optimal approach, leading to better surgical outcomes.

Trial Registration:
ClinicalTrials.gov identifier NCT0333434.
Right ventricular-pulmonary artery coupling is an independent risk factor for coronary artery lesions in children with Kawasaki disease
Authors: Cao Ran , Wu Xuchu , Zheng Xiaozhi 
Published: January 2024

Background
The recognition ability of right ventricular-pulmonary artery (RV-PA) coupling for coronary artery lesions (CAL) in children with Kawasaki disease (KD) has not been well characterized. This study aimed to determine whether RV-PA coupling is an independent the risk factors for CAL in children with KD.

Methods
Between October 2021 and August 2023, RV-PA coupling was assessed in 59 KD children using the ratio between echocardiographic tricuspid annular plane systolic excursion and pulmonary artery systolic pressure (PASP). Multivariable logistic regression analysis was used to identify the independent risk factors for CAL among the demographic, clinical, laboratory and echocardiographic data.

Results
Twenty-nine of 59 KD children had CAL according to the diagnostic criteria of echocardiography. There were significantly different white blood cell count, C-reactive protein, erythrocyte sedimentation rate, left ventricular ejection fraction, PASP and RV-PA coupling at admission, and significantly different acute/subacute phase ratio of RV-PA coupling between KD children with and without CAL (P < 0.05). Multivariate logistic regression analysis identified that acute/subacute phase ratio of RV-PA coupling (OR = 26.800; 95% CI, 1.276–562.668; P = 0.034) was an independent risk factor for CAL in children with KD. The area under receiver operating characteristic curve for the acute/subacute phase ratio of RV-PA coupling was 0.715 (95%CI: 0.624 - 0.825) to predict CAL in KD children (P < 0.05), with a sensitivity of 81.25% and a specificity of 62.57% at the optimal cutoff value of 0.839.

Conclusion
The acute/subacute phase ratio of RV-PA coupling was an independent risk factor for CAL in KD children.
An unusual imaging presentation of pediatric bacterial meningoencephalitis: a case-report study
Authors: Kachuei Maryam , Zare Ramin , Sayyahfar Shirin , Khalili Mitra , Movahedi Hamidreza , Naghshbandi Mobin , Eghdami Shayan 
Published: January 2024

Background:
Bacterial meningoencephalitis is a serious infection affecting the brain and its surrounding membranes. While imaging studies play a crucial role in diagnosing this condition, the typical radiological findings are well-documented. However, this case report describes an unusual imaging presentation that deviates from the expected patterns, emphasizing the need for awareness of such variations.

Case presentation:
A 7-year-old female with no prior medical history was referred to our hospital with fever, seizure, and loss of Consciousness. She had mild flu a week before admission. The duration of seizure episodes were 2–3 min, with tonic-clonic uncontrollable jerky movements. Brudzinski and Kernig signs were positive and plantar reflex was upward bilaterally in the physical examination. The computed tomography (CT) scan showed brain ventriculomegaly/hydrocephalus, and MRI findings indicated multiple foci located at cerebellum, basal ganglia, and thalamus alongside intensely restricted diffusion of the layering debris, suggesting pyogenic ventriculitis. Cerebrospinal fluid (CSF) analysis showed severe hypoglycorrhachia, despite non-significant increase of protein. The patient was undergone antibiotic therapy with ceftriaxone, vancomycin and rifampin, resulting in normalization of CSF values.

Conclusion:
This case report highlights the importance of recognizing and interpreting unusual imaging presentations of bacterial meningoencephalitis in paediatric patients. It emphasizes the need for a comprehensive diagnostic approach, including clinical evaluation, laboratory tests, and imaging studies, to ensure accurate diagnosis and appropriate management of this potentially life-threatening condition. Further research and awareness of atypical imaging findings are warranted to enhance our understanding and improve patient outcomes.
Debriefing Methods for Simulation in Healthcare
Authors: Duff Jonathan P. , Morse Kate J. , Seelandt Julia , Gross Isabel T. , Lydston Melis , Sargeant Joan , Dieckmann Peter , Allen Joseph A. , Rudolph Jenny W. , Kolbe Michaela 
Published: January 2024

Abstract
Debriefing is a critical component in most simulation experiences. With the growing number of debriefing concepts, approaches, and tools, we need to understand how to debrief most effectively because there is little empiric evidence to guide us in their use. This systematic review explores the current literature on debriefing in healthcare simulation education to understand the evidence behind practice and clarify gaps in the literature. The PICO question for this review was defined as “In healthcare providers [P], does the use of one debriefing or feedback intervention [I], compared to a different debriefing or feedback intervention [C], improve educational and clinical outcomes [O] in simulation-based education?” We included 70 studies in our final review and found that our current debriefing strategies, frameworks, and techniques are not based on robust empirical evidence. Based on this, we highlight future research needs.
Healthcare Simulation Training Guidelines and Literature Reviews From the Third Society for Simulation in Healthcare Research Summit
Authors: Scerbo Mark W. 
Published: January 2024

Abstract
The Society for Simulation in Healthcare held its third research summit in January 2023 with the aim of establishing evidence-based guidelines for healthcare simulation training. A panel of researchers, clinicians, and subject-matter experts conducted reviews of the literature addressing 12 key topics and followed a formal process to generate 16 guidelines for simulation-based training in healthcare. Eleven peer-reviewed literature reviews accompany these guidelines. Over the last 12 years, the Society for Simulation in Healthcare research summits have evolved with a consistent aim to advance simulation research, culminating in the formal set of guidelines published in this special issue.