bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guilt, Fear, Stigma and Knowledge Gaps: Ethical Issues in Public Health Communication Interventions
Authors: Guttman Nurit , Salmon Charles T. 
Published: October 2004

ABSTRACTPublic health communication campaigns have been credited with helping raise awareness of risk from chronic illness and new infectious diseases and with helping promote the adoption of recommended treatment regimens. Yet many aspects of public health communication interventions have escaped the scrutiny of ethical discussions. With the transference of successful commercial marketing communication tactics to the realm of public health, consideration of ethical issues becomes an essential component in the development and application of public health strategies. Ethical issues in public health communication are explored as they relate to eight topics: ‘targeting’ and ‘tailoring’ public health messages to particular population segments; obtaining the equivalence of informed consent; the use of persuasive communication tactics; messages on responsibility and culpability; messages that apply to harm reduction; and three types of unintended adverse effects associated with public health communication activities that may label and stigmatise, expand social gaps, and promote health as a value. We suggest that an ethical analysis should be applied to each phase of the public health communication process in order to identify ethical dilemmas that may appear subtle, yet reflect important concerns regarding potential effects of public health communication interventions on individuals and society as a whole.
Biogas as Fuel for a Fuel Cell System: Investigations and First Experimental Results for a Molten Carbonate Fuel Cell
Authors: Bove Roberto , Lunghi Piero , Lutazi Alessio , Sammes Nigel M. 
Published: October 2004

AbstractThe environmental benefits related to fuel cells can be enhanced if hydrogen is produced through an environmental friendly practice. Gasification has been widely usied for decades for syngas production from coal, by the so called Integrated Gasification Combined Plants (IGCC), allowing a dramatic emissions reduction, compared to combustion. For these two reasons, several authors have analyzed the possibility of realizing gasification plants coupled with fuel cell systems and, eventually a bottoming cycle. If the gas, however, is produced using a renewable source of energy, such as biomass, the related emissions reduction and fossil fuel preservation represent a further improvement for the environmental performance of the system. In the present study, advantages of an MCFC biogas fueled system is discussed, along with the technical problems related with the integration of the FC section with a Gasifier. Finally, experimental results conducted on a single cell are reported.
Mathematical Model of a Direct Methanol Fuel Cell
Authors: García Brenda L. , Sethuraman Vijay A. , Weidner John W. , White Ralph E. , Dougal Roger 
Published: October 2004

AbstractA one-dimensional, isothermal model for a direct methanol fuel cell (DMFC) is presented. This model accounts for the kinetics of the multi-step methanol oxidation reaction at the anode. Diffusion and crossover of methanol are modeled and the mixed potential of the oxygen cathode due to methanol crossover is included. Kinetic and diffusional parameters are estimated by comparing the model to data from a 25cm2 DMFC. This semi-analytical model can be solved rapidly so that it is suitable for inclusion in real-time system level DMFC simulations.
Familial risk of urinary incontinence in women: population based cross sectional study
Authors: Hannestad Yngvild S , Lie Rolv Terje , Rortveit Guri , Hunskaar Steinar 
Published: October 2004

Abstract

Objective
To determine whether there is an increased risk of urinary incontinence in daughters and sisters of incontinent women.

Design
Population based cross sectional study.

Setting
EPINCONT (the epidemiology of incontinence in the county of Nord-Trøndelag study), a substudy of HUNT 2 (the Norwegian Nord-Trøndelag health survey 2), 1995-7.

Participants
6021 mothers, 7629 daughters, 332 granddaughters, and 2104 older sisters of 2426 sisters.

Main outcome measures
Adjusted relative risks for urinary incontinence.

Results
The daughters of mothers with urinary incontinence had an increased risk for urinary incontinence (1.3, 95% confidence interval 1.2 to 1.4; absolute risk 23.3%), stress incontinence (1.5, 1.3 to 1.8; 14.6%), mixed incontinence (1.6, 1.2 to 2.0; 8.3%), and urge incontinence (1.8, 0.8 to 3.9; 2.6%). If mothers had severe symptoms then their daughters were likely to have such symptoms (1.9, 1.3 to 3.0; 4.0%). The younger sisters of female siblings with urinary incontinence, stress incontinence, or mixed incontinence had increased relative risks of, respectively, 1.6 (1.3 to 1.9; absolute risk 29.6%), 1.8 (1.3 to 2.3; 18.3%), and 1.7 (1.1 to 2.8; 10.8%).

Conclusion
Women are more likely to develop urinary incontinence if their mother or older sisters are incontinent.
Introduction of nurse led DC cardioversion service in day surgery unit: prospective audit
Authors: Currie M P , Karwatowski S P , Perera J , Langford E J 
Published: October 2004

Abstract

Problem
Atrial fibrillation is the most common persistent arrhythmia in adults and carries an increased risk of thromboembolism and stroke. Electrical (DC) cardioversion is an effective treatment, but logistical difficulties in many institutions lead to problems providing a prompt service. This reduces the rate of long term success, delays relief of symptoms, and increases the burden on anticoagulation clinics.

Design
Prospective audit of introduction of a collaborative, nurse led DC cardioversion service in a day surgery unit.

Setting
Day surgery unit 5 km from an acute hospital in southeast London.

Key measures for improvement
Waiting times, success of procedures, and complication rates.

Strategies for change
Collaborative working across traditional specialty boundaries; empowerment of patients within the process; using a nurse consultant as a single point of reference to coordinate the service.

Effects of change
Sinus rhythm was restored in 131 (92%) of the first 143 patients treated. Three patients needed hospital admission; all were discharged uneventfully within 24 hours. No important complications occurred. Waiting times were reduced from 27 weeks to eight weeks for patients eligible for the service.

Lessons learnt
Elective DC cardioversion under general anaesthesia can be safely done by an appropriately trained nurse in a day surgery unit remote from an acute general hospital. This model of care is effective and can reduce waiting times and relieve pressure on acute beds and junior doctors.
Tragic Re-Presentation and the Semantics of Space in Plautus' Casina
Authors:   Andrews
Published: October 2004

AbstractThis article examines motivations for tragic parody in Plautus' Casina, 621 ff. It details how the Casina, and Pardalisca's parodic scene in particular, treat themes common to Greek tragedy—the spatial opposition between male and female worlds, the on-stage struggle for power between men and women, and female madness—and also explores Plautus' comic manipulation of Aristotle's tragic theory. Through her tragic performance, Pardalisca provides a very different perspective on the absent Casina from the one provided thus far in the play and believably represents Casina's possible actions and state of mind. Pardalisca transgresses the conventional boundaries between male/outside and female/inside established early in the play, and she uses the public space outside of the house to externalize and legitimize the private concerns of women inside the house, and to rupture the physical and emotional constraints on women through her paratragic scene. The final 'wedding' scene then comically mirrors Pardalisca's tragic scene and provides a glimpse of the sexual violence Casina might have been compelled to endure had she been forced to comply with Lysidamus' plan, yet neatly turns male violence back on male characters and makes them the comic victims, not just of the women characters, but of themselves.
Φapmakeytpia Ohne Φapmakon. Überlegungen zur Komposition des zweiten Idylls von Theokrit
Authors:   Petrovic
Published: October 2004

AbstractIn the first part of the article, the question of Theocritus' treatment of magic in the 2nd Idyll is being discussed. The author's view is that Theocritus not only accurately depicted the contemporary magical practices, but that the very composition of the poem was influenced by the magical texts. The second part of the paper pays attention to a special class of magical texts, the defixiones influenced by the prayers for justice. The author argues that Simaetha's confession to Selene bears close resemblance to the prayers for justice and that Simaetha's argumentative strategy in the description of her short love affair with Delphis serves the purpose of portraying her as the betrayed party, thus enabling her to put the blame on Delphis and demand of the goddess that the justice (e.g. the return of Delphis to Simaetha) should be done. Finally, the effect of Simaetha's magical practice and her prayer is being discussed. Since the motif of ϕαρμακον connects the 2nd with the 11th Idyll, a comparison between Simaetha and Cyclops is called for and some general conclusions about the motif of the healing properties of poetry in the Theocritean corpus are suggested.
The Origin of the Kabeiroi
Authors:   Beekes
Published: October 2004

AbstractIt is argued that Kadmilos, one of the Kabeiroi, has a typical (non-IE) Anatolian name. And further that the name Kabeiroi itself is a variant of Kabarn-oi. New insight in Pre-Greek shows that this word is a typical Pre-Greek name, and that the original form is * Kabar(y)-. This shows that the old connection with Semitic (kabir- 'great') must be definitely given up.
Content-rich biological network constructed by mining PubMed abstracts
Authors: Chen Hao , Sharp Burt M 
Published: October 2004

Abstract
Background
The integration of the rapidly expanding corpus of information about the genome, transcriptome, and proteome, engendered by powerful technological advances, such as microarrays, and the availability of genomic sequence from multiple species, challenges the grasp and comprehension of the scientific community. Despite the existence of text-mining methods that identify biological relationships based on the textual co-occurrence of gene/protein terms or similarities in abstract texts, knowledge of the underlying molecular connections on a large scale, which is prerequisite to understanding novel biological processes, lags far behind the accumulation of data. While computationally efficient, the co-occurrence-based approaches fail to characterize (e.g., inhibition or stimulation, directionality) biological interactions. Programs with natural language processing (NLP) capability have been created to address these limitations, however, they are in general not readily accessible to the public.

Results
We present a NLP-based text-mining approach, Chilibot, which constructs content-rich relationship networks among biological concepts, genes, proteins, or drugs. Amongst its features, suggestions for new hypotheses can be generated. Lastly, we provide evidence that the connectivity of molecular networks extracted from the biological literature follows the power-law distribution, indicating scale-free topologies consistent with the results of previous experimental analyses.

Conclusions
Chilibot distills scientific relationships from knowledge available throughout a wide range of biological domains and presents these in a content-rich graphical format, thus integrating general biomedical knowledge with the specialized knowledge and interests of the user. Chilibot http://www.chilibot.net can be accessed free of charge to academic users.
Cdc42 Effector Protein 2 (XCEP2) is required for normal gastrulation and contributes to cellular adhesion in Xenopus laevis
Authors: Nelson Karen K , Nelson Richard W 
Published: October 2004

Abstract
Background
Rho GTPases and their downstream effector proteins regulate a diverse array of cellular processes during embryonic development, including reorganization of cytoskeletal architecture, cell adhesion, and transcription. Changes in the activation state of Rho GTPases are converted into changes in cellular behavior by a diversity of effector proteins, which are activated in response to changes in the GTP binding state of Rho GTPases. In this study we characterize the expression and function of one such effector, XCEP2, that is present during gastrulation stages in Xenopus laevis.

Results
In a search for genes whose expression is regulated during early stages of embryonic development in Xenopus laevis, a gene encoding a Rho GTPase effector protein (Xenopus Cdc42 effector protein 2, or XCEP2) was isolated, and found to be highly homologous, but not identical, to a Xenopus sequence previously submitted to the Genbank database. These two gene sequences are likely pseudoalleles. XCEP2 mRNA is expressed at constant levels until mid- to late- gastrula stages, and then strongly down-regulated at late gastrula/early neurula stages. Injection of antisense morpholino oligonucleotides directed at one or both pseudoalleles resulted in a significant delay in blastopore closure and interfered with normal embryonic elongation, suggesting a role for XCEP2 in regulating gastrulation movements. The morpholino antisense effect could be rescued by co-injection with a morpholino-insensitive version of the XCEP2 mRNA. Antisense morpholino oligonucleotides were found to have no effect on mesodermal induction, suggesting that the observed effects were due to changes in the behavior of involuting cells, rather than alterations in their identity. XCEP2 antisense morpholino oligonucleotides were also observed to cause complete disaggregation of cells composing animal cap explants, suggesting a specific role of XCEP2 in maintenance or regulation of cell-cell adhesion in early embryos. This loss of cell adhesion could be rescued by co-injection with a morpholino-insensitive version of the XCEP2 mRNA.

Conclusions
XCEP2 appears to be an essential component in the early developmental program in Xenopus laevis. XCEP2 is involved in maintenance of cell-cell adhesion, and as such may constitute a regulatory component that could help to balance the need for tissue integrity and plasticity during the dynamic cellular rearrangements of gastrulation.
Genome-wide microarray analysis of TGFβ signaling in the Drosophilabrain
Authors: Yang Maocheng , Nelson Don , Funakoshi Yoko , Padgett Richard W 
Published: October 2004

Abstract
Background
Members of TGFβ superfamily are found to play important roles in many cellular processes, such as proliferation, differentiation, development, apoptosis, and cancer. In Drosophila, there are seven ligands that function through combinations of three type I receptors and two type II receptors. These signals can be roughly grouped into two major TGFβ pathways, the dpp/BMP and activin pathways, which signal primarily through thick veins (tkv) and baboon (babo). Few downstream targets are known for either pathway, especially targets expressed in the Drosophila brain.

Results
tkv and babo both affect the growth of tissues, but have varying effects on patterning. We have identified targets for the tkv and babo pathways by employing microarray techniques using activated forms of the receptors expressed in the brain. In these experiments, we compare the similarities of target genes of these two pathways in the brain. About 500 of 13,500 examined genes changed expression at 95% confidence level (P < 0.05). Twenty-seven genes are co-regulated 1.5 fold by both the tkv and babo pathways. These regulated genes cluster into various functional groups such as DNA/RNA binding, signal transducers, enzymes, transcription regulators, and neuronal regulators. RNAi knockdown experiments of homologs of several of these genes show abnormal growth regulation, suggesting these genes may execute the growth properties of TGFβ.

Conclusions
Our genomic-wide microarray analysis has revealed common targets for the tkv and babo pathways and provided new insights into downstream effectors of two distinct TGFβ like pathways. Many of these genes are novel and several genes are implicated in growth control. Among the genes regulated by both pathways is ultraspiracle, which further connects TGFβ with neuronal remodeling.
Characterization of an endogenous retrovirus class in elephants and their relatives
Authors: Greenwood Alex D , Englbrecht Claudia C , MacPhee Ross DE 
Published: October 2004

Abstract
Background
Endogenous retrovirus-like elements (ERV-Ls, primed with tRNA leucine) are a diverse group of reiterated sequences related to foamy viruses and widely distributed among mammals. As shown in previous investigations, in many primates and rodents this class of elements has remained transpositionally active, as reflected by increased copy number and high sequence diversity within and among taxa.

Results
Here we examine whether proviral-like sequences may be suitable molecular probes for investigating the phylogeny of groups known to have high element diversity. As a test we characterized ERV-Ls occurring in a sample of extant members of superorder Uranotheria (Asian and African elephants, manatees, and hyraxes). The ERV-L complement in this group is even more diverse than previously suspected, and there is sequence evidence for active expansion, particularly in elephantids. Many of the elements characterized have protein coding potential suggestive of activity.

Conclusions
In general, the evidence supports the hypothesis that the complement had a single origin within basal Uranotheria.
Evolutionary relationships of Aurora kinases: Implications for model organism studies and the development of anti-cancer drugs
Authors: Brown James R , Koretke Kristin K , Birkeland Marian L , Sanseau Philippe , Patrick Denis R 
Published: October 2004

Abstract
Background
As key regulators of mitotic chromosome segregation, the Aurora family of serine/threonine kinases play an important role in cell division. Abnormalities in Aurora kinases have been strongly linked with cancer, which has lead to the recent development of new classes of anti-cancer drugs that specifically target the ATP-binding domain of these kinases. From an evolutionary perspective, the species distribution of the Aurora kinase family is complex. Mammals uniquely have three Aurora kinases, Aurora-A, Aurora-B, and Aurora-C, while for other metazoans, including the frog, fruitfly and nematode, only Aurora-A and Aurora-B kinases are known. The fungi have a single Aurora-like homolog. Based on the tacit assumption of orthology to human counterparts, model organism studies have been central to the functional characterization of Aurora kinases. However, the ortholog and paralog relationships of these kinases across various species have not been rigorously examined. Here, we present comprehensive evolutionary analyses of the Aurora kinase family.

Results
Phylogenetic trees suggest that all three vertebrate Auroras evolved from a single urochordate ancestor. Specifically, Aurora-A is an orthologous lineage in cold-blooded vertebrates and mammals, while structurally similar Aurora-B and Aurora-C evolved more recently in mammals from a duplication of an ancestral Aurora-B/C gene found in cold-blooded vertebrates. All so-called Aurora-A and Aurora-B kinases of non-chordates are ancestral to the clade of chordate Auroras and, therefore, are not strictly orthologous to vertebrate counterparts. Comparisons of human Aurora-B and Aurora-C sequences to the resolved 3D structure of human Aurora-A lends further support to the evolutionary scenario that vertebrate Aurora-B and Aurora-C are closely related paralogs. Of the 26 residues lining the ATP-binding active site, only three were variant and all were specific to Aurora-A.

Conclusions
In this study, we found that invertebrate Aurora-A and Aurora-B kinases are highly divergent protein families from their chordate counterparts. Furthermore, while the Aurora-A family is ubiquitous among all vertebrates, the Aurora-B and Aurora-C families in humans arose from a gene duplication event in mammals. These findings show the importance of understanding evolutionary relationships in the interpretation and transference of knowledge from studies of model organism systems to human cellular biology. In addition, given the important role of Aurora kinases in cancer, evolutionary analysis and comparisons of ATP-binding domains suggest a rationale for designing dual action anti-tumor drugs that inhibit both Aurora-B and Aurora-C kinases.
Variation suggestive of horizontal gene transfer at a lipopolysaccharide (lps) biosynthetic locus in Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen of rice
Authors: Patil Prabhu B , Sonti Ramesh V 
Published: October 2004

Abstract
Background
In animal pathogenic bacteria, horizontal gene transfer events (HGT) have been frequently observed in genomic regions that encode functions involved in biosynthesis of the outer membrane located lipopolysaccharide (LPS). As a result, different strains of the same pathogen can have substantially different lps biosynthetic gene clusters. Since LPS is highly antigenic, the variation at lps loci is attributed to be of advantage in evading the host immune system. Although LPS has been suggested as a potentiator of plant defense responses, interstrain variation at lps biosynthetic gene clusters has not been reported for any plant pathogenic bacterium.

Results
We report here the complete sequence of a 12.2 kb virulence locus of Xanthomonas oryzae pv. oryzae (Xoo) encoding six genes whose products are homologous to functions involved in LPS biosynthesis and transport. All six open reading frames (ORFs) have atypical G+C content and altered codon usage, which are the hallmarks of genomic islands that are acquired by horizontal gene transfer. The lps locus is flanked by highly conserved genes, metB and etfA, respectively encoding cystathionine gamma lyase and electron transport flavoprotein. Interestingly, two different sets of lps genes are present at this locus in the plant pathogens, Xanthomonas campestris pv. campestris (Xcc) and Xanthomonas axonopodis pv. citri (Xac). The genomic island is present in a number of Xoo strains from India and other Asian countries but is not present in two strains, one from India (BXO8) and another from Nepal (Nepal624) as well as the closely related rice pathogen, Xanthomonas oryzae pv. oryzicola (Xoor). TAIL-PCR analysis indicates that sequences related to Xac are present at the lps locus in both BXO8 and Nepal624. The Xoor strain has a hybrid lps gene cluster, with sequences at the metB and etfA ends, being most closely related to sequences from Xac and the tomato pathogen, Pseudomonas syringae pv. tomato respectively.

Conclusion
This is the first report of hypervariation at an lps locus between different strains of a plant pathogenic bacterium. Our results indicate that multiple HGT events have occurred at this locus in the xanthomonad group of plant pathogens.