bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental analysis of voice volume for children with autism spectrum disorder
Authors: Garcia Arturo , Wunderlich Kara L. , Pelfrey Chandler , Sheppard Christina M. 
Published: October 2023

AbstractInappropriate prosodic production, or features of speech, is an often observed but rarely treated communication skill deficit in individuals with autism. Expanding on previous literature, we conducted a functional analysis (FA) on the voice volume responses (VVRs) of two children with autism spectrum disorder utilizing similar procedures to those of Edgerton and Wine. Then, we evaluated the efficacy of visual feedback from an app and a function‐based treatment to decrease loud VVRs and increase appropriate VVRs. Results of the FA indicated loud VVRs were maintained by social negative reinforcement for one participant and by both social negative and automatic reinforcement for another. For one participant, the intervention decreased the use of loud VVRs and increased appropriate VVRs. For the other participant, the results of the intervention were inconclusive. Implications of conducting functional analyses and behavioral interventions to treat inappropriate voice volume are discussed.
Enzyme‐functionalized alginate microparticles enable anaerobic culture under ambient oxygen
Authors: Williams Tyrell J. , Daboin Claudia , Kim Paul , Guo Fengguang , de Figueiredo Paul , Alge Daniel L. 
Published: October 2023

AbstractMethods for culturing oxygen‐sensitive cells and organisms under anaerobic conditions are vital to biotechnology research. Here, we report a biomaterial‐based platform for anaerobic culture that consists of glucose oxidase (GOX) functionalized alginate microparticles (ALG‐GOX), which are designed to deplete dissolved [O2] through enzymatic activity. ALG‐GOX microparticles were synthesized via a water‐in‐oil emulsion and had a size of 132.0 ± 51.4 µm. Despite having a low storage modulus, the microparticles remained stable under aqueous conditions due to covalent crosslinking through amide bonds. Enzyme activity was tunable based on the loaded GOX concentration, with a maximum activity of 3.6 ± 0.3 units/mg of microparticles being achieved at an initial loading concentration of 5 mg/mL of GOX in alginate precursor solution. High enzyme activity in ALG‐GOX microparticles resulted in rapid oxygen depletion, producing a suitable environment for anaerobic culture. Microparticles loaded with both GOX and catalase (ALG‐GOX‐CAT) to reduce H2O2 buildup exhibited sustained activity for potential long‐term anaerobic culture. ALG‐GOX‐CAT microparticles were highly effective for the anaerobic culture of Bacteroides thetaiotaomicron, with 10 mg/mL of ALG‐GOX‐CAT microparticles supporting the same level of growth in an aerobic environment compared to an anaerobic chamber after 16 h (8.70 ± 0.96 and 10.03 ± 1.03 million CFU, respectively; N.S. p = 0.07). These microparticles could be a valuable tool for research and development in biotechnology.
Recurrent toxic epidermal necrolysis with two different drugs in a patient with chronic kidney disease–A case report with an atypical presentation
Authors: Mathur Mahesh , Thakur Neha , Jaiswal Sunil , Maharjan Srijana , Paudel Supriya , Shrestha Anjali 
Published: October 2023

AbstractToxic epidermal necrolysis (TEN) is a dermatologic emergency usually attributed to drugs. Recurrent episodes of TEN are more common in the pediatric population than in adults. Patients carrying susceptible specific haplotypes, cross‐reactivity between the drugs, and drug metabolites generated by the Cytochrome P450 are the key factors for the recurrent episodes. Chronic kidney disease (CKD) increases the risk of toxic epidermal necrolysis by altering the pharmacokinetics and pharmacodynamics of the drug with comparatively higher mortality in this group of patients. We hereby present an elderly female with 2 episodes of TEN following intake of furosemide at present and Nimesulide 3 years back. Cross‐reactivity between these drugs because of the similar stereochemical structure might have triggered the second episode. The second episode of TEN was milder in presentation with a short latency period without any constitutional symptoms as compared to the first episode. Thus, treating physicians should always consider cross‐reactivity between the chosen drugs in order to prevent subsequent life‐threatening episodes, especially in patients with CKD.
Recent Advances in Non‐Precious Metal Single‐Atom Electrocatalysts for Oxygen Reduction Reaction in Low‐Temperature Polymer‐Electrolyte Fuel Cells
Authors: Sarapuu Ave , Lilloja Jaana , Akula Srinu , Zagal José H. , Specchia Stefania , Tammeveski Kaido 
Published: October 2023

AbstractFuel cells have emerged as a promising clean electrochemical energy technology with a great potential in various sectors, including transportation and power generation. However, the high cost and scarcity of the noble metals currently used to synthesise electrocatalysts for low‐temperature fuel cells has hindered their widespread commercialisation. In recent decades, the development of non‐precious metal electrocatalysts for the cathodic oxygen reduction reaction (ORR) have gained significant attention. Among those, electrocatalysts with atomically dispersed active sites, referred to as single‐atom catalysts (SACs), are gaining more interest. Nanocarbon materials containing single transition metal atoms coordinated to nitrogen atoms are active electrocatalysts for the ORR in both acidic and alkaline conditions and thus have a great promise to be utilised as non‐precious metal cathode electrocatalysts in low‐temperature fuel cells. This review article provides an overview of the recent advancements in the utilisation of transition metal‐based SACs in proton exchange membrane fuel cells (PEMFCs) and anion exchange membrane fuel cells (AEMFCs). We highlight the main strategies and synthetic approaches for tailoring the properties of SACs to enhance their ORR activity and durability. Based on the already achieved results, it is evident that SACs indeed could be suitable for the cathode of the low‐temperature fuel cells.
Quantitative Analysis of Cellular Morphology During In Vitro Decidualization
Authors: Ant Luciana , Dily François Le , Beato Miguel , Saragüeta Patricia 
Published: October 2023

AbstractDecidualization is a differentiation process involving shape reorganization from a fibroblast to an epithelioid‐like appearance characteristic of endometrial stromal cells. For the study of in vitro decidualization, one needs to check that the cells have undergone this process effectively. Verification is usually done by analyzing the expression of decidual markers, but changes in morphology are a more comprehensive feature. However, morphological specificities (i.e., flatness) of endometrial cells prevent the use of existing automated tools. A simple and accurate methodology was developed to quantify the phenotypic changes that occur in an in vitro decidualization system. This approach analyzes cell circularity directly from light microscopy images to follow the effects of progesterone or progestin R5020 in combination with estradiol (E2) and cAMP in inducing the decidualization of human endometrial cells. A statistical model to detect the differences in the kinetics of decidualization of the two hormonal stimuli before all the cell population acquire the decidual phenotype was implemented. It was found that statistical differences in morphology between decidualized and control cells could be detected 2 days after the treatments. Here we detail the model applied, scripts, and input files in order to provide a useful, practical, and low‐cost tool to evaluate morphological aspects of endometrial stromal differentiation. This method allows the verification of the effectiveness of the decidualization process of the stromal endometrial cells without having to use cell replicates, as other methods such as immunofluorescence and RT‐qPCR assays require. Consequently, this approach can follow the kinetics of a living single replicate throughout the experiment. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Cell circularity quantification of human stromal endometrial cells using ImageJBasic Protocol 2: Statistical analysis of cell circularity of human stromal endometrial cells
Risk of malignancy assessment of the different cytologic categories in respiratory cytology samples according to the new guidelines of the Papanicolaou Society of Cytopathology
Authors: Ardor Gokce Deniz , Ahmed Momin , Hanna Karina , Ibanoglu Onur , Nassar Aziza 
Published: October 2023

AbstractBackgroundCytology is extremely important for diagnosis of lung carcinoma and the Papanicolaou Society of Cytopathology (PSC) had proposed a new classification system for respiratory cytology for better communication between physicians and better patient management. The objective of this study is to analyze our samples in accordance with this classification and to evaluate the diagnostic accuracy of various cytologic techniques and to assess the risk of malignancy.MethodsEight hundred and twenty respiratory cytology specimens (FNA, BAL, washing, brushing, sputum) collected between 2019 and 2022 were classified according to the PSC system and the risk of malignancy was assessed for each category using follow‐up surgical samples. Sensitivity, specificity, and accuracy rates were determined based on a categorial approach, according to a similar study.ResultsThe data of 820 respiratory cytology specimens from 576 patients were analyzed. 2.6% of these were non‐diagnostic, 64.1% were NM, 5% were AC, 0.4% were N‐B‐LG, 4% were SM and 23.9% were ML. The risk of malignancy for each diagnostic category were: 42.8% for non‐diagnostic, 31.2% for NM, 43.9% for AC, 87.9% for SM, 94.3% for ML. Sensitivity and specificity was calculated using only the malignant cases considered as positive tests and was 45.57% and 97.34% respectively.ConclusionOur results correlated with the PSC system, and it was considered useful in clinical practice. However, more studies should be performed to evaluate the usefulness of this system. The ROMs of each category and the impact of different techniques should be further studied.
Diurnal circadian clock gene expression is altered in models of genetic and acquired epilepsy
Authors: Yamakawa Glenn R. , Patel Meshwa , Lin Runxuan , O'Brien Terence J. , Mychasiuk Richelle , Casillas‐Espinosa Pablo M. 
Published: October 2023

AbstractObjectivesGrowing evidence demonstrates a relationship between epilepsy and the circadian system. However, relatively little is known about circadian function in disease states, such as epilepsy. This study aimed to characterize brain and peripheral core circadian clock gene expression in rat models of genetic and acquired epilepsy.MethodsFor the Genetic Absence Epilepsy Rats from Strasbourg (GAERS) study, we used 40 GAERS and 40 non‐epileptic control (NEC) rats. For the kainic acid status epilepticus (KASE) study, we used 40 KASE and 40 sham rats. Rats were housed in a 7 am:7 pm light–dark cycle. Hypothalamus, hippocampus, liver, and small intestine samples were collected every 3 h throughout the light period. We then assessed core diurnal clock gene expression of per1, cry1, clock, and bmal1.ResultsIn the GAERS rats, all tissues exhibited significant changes in clock gene expression (P < 0.05) when compared to NEC. In the KASE rats, there were fewer effects of the epileptic condition in the hypothalamus, hippocampus, or small intestine (P > 0.05) compared with shams.SignificanceThese results indicate marked diurnal disruption to core circadian clock gene expression in rats with both generalized and focal chronic epilepsy. This could contribute to epileptic symptomology and implicate the circadian system as a viable target for future treatments.
On the structure of consistent cycles in cubic symmetric graphs
Authors: Kutnar Klavdija , Marušič Dragan , Miklavič Štefko , Šparl Primož 
Published: October 2023

AbstractA cycle in a graph is consistent if the automorphism group of the graph admits a one‐step rotation of this cycle. A thorough description of consistent cycles of arc‐transitive subgroups in the full automorphism groups of finite cubic symmetric graphs is given.
Magnetic resonance imaging‐based bone imaging of the lower limb: Strategies for generating high‐resolution synthetic computed tomography
Authors: Florkow Mateusz C. , Nguyen Chien H. , Sakkers Ralph J. B. , Weinans Harrie , Jansen Mylene P. , Custers Roel J. H. , van Stralen Marijn , Seevinck Peter R. 
Published: October 2023

AbstractThis study aims at assessing approaches for generating high‐resolution magnetic resonance imaging‐ (MRI‐) based synthetic computed tomography (sCT) images suitable for orthopedic care using a deep learning model trained on low‐resolution computed tomography (CT) data. To that end, paired MRI and CT data of three anatomical regions were used: high‐resolution knee and ankle data, and low‐resolution hip data. Four experiments were conducted to investigate the impact of low‐resolution training CT data on sCT generation and to find ways to train models on low‐resolution data while providing high‐resolution sCT images. Experiments included resampling of the training data or augmentation of the low‐resolution data with high‐resolution data. Training sCT generation models using low‐resolution CT data resulted in blurry sCT images. By resampling the MRI/CT pairs before the training, models generated sharper images, presumably through an increase in the MRI/CT mutual information. Alternatively, augmenting the low‐resolution with high‐resolution data improved sCT in terms of mean absolute error proportionally to the amount of high‐resolution data. Overall, the morphological accuracy was satisfactory as assessed by an average intermodal distance between joint centers ranging from 0.7 to 1.2 mm and by an average intermodal root‐mean‐squared distances between bone surfaces under 0.7 mm. Average dice scores ranged from 79.8% to 87.3% for bony structures. To conclude, this paper proposed approaches to generate high‐resolution sCT suitable for orthopedic care using low‐resolution data. This can generalize the use of sCT for imaging the musculoskeletal system, paving the way for an MR‐only imaging with simplified logistics and no ionizing radiation.
Subacromial contact after acromioplasty in the rotator cuff deficient shoulder
Authors: Gatto Laura , Fernando Ashen , Patel Minoo , Yeung Angus , Ackland David C. 
Published: October 2023

AbstractSubacromial impingement (SAI) is associated with shoulder pain and dysfunction and is exacerbated by rotator cuff tears; however, the role of acromioplasty in mitigating subacromial contact in the rotator cuff deficient shoulder remains debated. This study aimed to quantify the influence of isolated and combined tears involving the supraspinatus on subacromial contact during abduction; and second, to evaluate the influence of acromioplasty on joint space size and subacromial contact under these pathological conditions. Eight fresh‐frozen human cadaveric upper limbs were mounted to a computer‐controlled testing apparatus that simulated joint motion by simulated force application. Shoulder abduction was performed while three‐dimensional joint kinematics was measured using an optoelectronic system, and subacromial contact evaluated using a digital pressure sensor secured to the inferior acromion. Testing was performed after an isolated tear to the supraspinatus, as well as tears involving the subscapularis and infraspinatus‐teres minor, both before and after acromioplasty. Rotator cuff tears significantly increased peak subacromial pressure (p < 0.001), average subacromial pressure (p = 0.001), and contact force (p = 0.034) relative to those in the intact shoulder. Following acromioplasty, significantly lower peak subacromial contact pressure, force and area were observed for all rotator cuff tears involving the supraspinatus at 30° of abduction (p < 0.05). Acromioplasty predominantly reduces acromion thickness anteriorly thereby reducing subacromial contact in the rotator cuff deficient shoulder, particularly in early to mid‐abduction where superior glenohumeral joint shear force potential is large. These findings provide a biomechanical basis for acromioplasty as an intervention for SAI syndrome and as an adjunct to rotator cuff repairs.
Surgical Approaches to Petrous Apex Cholesterol Granulomas: A Systematic Review and Network Meta‐analysis
Authors: Noy Roee , Bassal Raghad , Ostrovsky Dmitry , Cohen Jacob , Ishai Reuven , Cohen‐Vaizer Mauricio , Vaisbuch Yona 
Published: October 2023

ObjectiveTo compare the outcomes of different surgical approaches to petrous apex cholesterol granulomas (PACG).Data SourcesPubMed, Embase, Google Scholar, Cochrane, and Web of Science.Review MethodsFollowing the Preferred Reporting Items for Systematic Reviews and Meta‐analyses‐Network Meta‐analyses guidelines, databases were searched from inception to November 31, 2022. Studies comparing two or more approaches were included. Reviews and population studies were excluded. The main outcome measures were the resolution of symptoms, serviceable hearing, complication, and revision rates.ResultsThe search yielded 2132 studies. After applying inclusion and exclusion criteria, 15 studies remained, consisting of 214 patients treated with lateral approaches (n = 182) or anterior endonasal approaches (n = 32). The efficacy of lateral and anterior endonasal approaches in achieving symptom resolution was comparable (73% vs. 68%, p = 0.5). Both exhibited similar rates of complications (33% vs. 37%, p = 0.3), albeit with distinct profiles. Lateral approaches were associated with higher rates of facial palsy and sensorineural hearing loss (44% vs. 18%, p = 0.03). Anterior endonasal approaches demonstrated higher rates of epistaxis and cerebrospinal fluid leak (15% vs. 1%, p = 0.001). Anterior endonasal approaches exhibited lower revision rates (OR: 0.35, 95% CI: 0.14–0.88). The placement of a stent in both approaches was associated with higher symptom resolution (OR: 5.12, 95% CI: 1.05–9.97) and lower revision rates (OR: 0.71, 95% CI: 0.33–0.92).ConclusionsAnterior endonasal approaches yield lower revision rates compared to lateral approaches for PACG. Both approaches demonstrate similar effectiveness in symptom resolution and comparable rates of complications, with distinct profiles. Facial nerve and hearing status are important factors that should be addressed when selecting the approach. Stenting is beneficial.Level of EvidenceNA Laryngoscope, 134:1540–1550, 2024
A Dual Reversible Cross‐Linked Hydrogel with Enhanced Mechanical Property and Capable of Proangiogenic and Osteogenic Activities for Bone Defect Repair
Authors: Chen Kai , He Wenbao , Gao Wei , Wu Yan , Zhang Zhe , Liu Mingxiang , Hu Yunping , Xiao Xiufeng , Li Fuping , Feng Qian 
Published: October 2023

AbstractThe clinical treatment of bone defects presents ongoing challenges. One promising approach is bone tissue engineering (BTE), wherein hydrogels have garnered significant attention. However, the application of hydrogels in BTE is severely limited due to their poor mechanical properties, as well as their inferior proangiogenic and osteogenic activities. To address these limitations, our develop a dual cross‐linked alendronate (ALN)‐Ca2+/Mg2+‐doped sulfated hyaluronic acid (SHA@CM) hydrogel, using a one‐step mixing injection molding method known as “three‐in‐one” approach. This approach enabled the simultaneous formation of Schiff‐Base crosslinking and electric attraction‐based crosslinking within the hydrogel. The Schiff‐Base crosslinking contributed to the majority of the hydrogel's mechanical strength, while the electric attraction‐based crosslinking served as a release reservoir for Ca2+/Mg2+ and ALN, promoting enhanced osteogenic activities and providing additional mechanical reinforcement to the hydrogel. These experimental data demonstrates several favorable properties of the SHA@CM hydrogel, including satisfactory injectability, rapid gelation, self‐healing capacity, and excellent cytocompatibility. Moreover, the presence of sulfated groups and Mg2+ within the SHA@CM hydrogel exhibited pro‐angiogenic effects, while the controlled release of nanoparticles formed by Ca2+/Mg2+ and ALN further enhanced the osteogenesis of the hydrogel. Overall, these results indicate that the SHA@CM hydrogel holds significant potential for the clinical translation of BTE.
Ultrawideband 3‐D printed aperture‐shared conformal dual‐horn antenna
Authors: Wang Yifan , Gao Ping , Li Teng , Han Min , Dou Wenbin 
Published: October 2023

AbstractAn ultrawideband 3D printed aperture‐shared conformal dual‐horn antenna that is mounted on a cylindrical metal surface is proposed. The antenna system consists of an H‐plane horn antenna working at 0.5–5.5 GHz, namely Antenna A, an H‐plane horn antenna working at 5.5–16 GHz, namely Antenna B, and a diplexer. To validate the proposed aperture‐shared antenna, a prototype is 3D printed with surface metallization. A diplexer is used to connect Antenna A and Antenna B. Measured results show a 32:1 impedance bandwidth for the dual‐horn antenna system with |S11| < −6.8 dB. Good radiation patterns are also obtained for most of the bandwidth.
Spectral boundary integral equation method for simulation of 2D and 3D slip ruptures at bi‐material interfaces
Authors: Gupta Avinash , Ranjith Kunnath 
Published: October 2023

AbstractThis paper presents a 3D spectral numerical scheme that can accurately model planarcracks of any shape residing on an interfacial plane between two elastic solids. The cracks propagate dynamically under the action of interfacial loads and interfacial constitutive laws. The scheme is a spectral form of the boundary integral equations (BIE) that relate the displacement discontinuity fields along the interfacial plane to the stress fields at the plane. In the BIE, the displacement discontinuities are expressed as a spatio‐temporal convolution of the traction components at the interface. This is in contrast with most previous studies which use a spatio‐temporal convolution of the displacement discontinuities or of the displacements at the interface. Due to the continuity of tractions across the interface, the present formulation is simpler, resulting in convolution kernels that can be written in closed‐form. Previous studies have relied on numerically obtained convolution kernels. The accuracy of the proposed scheme is validated with the known analytical solution of the 3D Lamb problem. Furthermore, new algorithms are developed for coupling the BIE with a slip‐weakening friction law, both for homogeneous materials as well as for bi‐material interfaces. This results in a widely applicable formulation for studying spontaneous rupture propagation. The algorithms are validated by comparing rupture simulation exercises to benchmark problems from Southern California Earthquake Center (SCEC). Finally, the methodology is used to simulate 3D frictional rupture propagation along a bi‐material interface.