bluuebunny/crossref_metadata_2025_split huggingface dataset:
Restoring Impaired Neurogenesis and Alleviating Oxidative Stress by
Cyanidin against Bisphenol A-induced Neurotoxicity: In Vivo and In Vitro
Evidence
Authors: Suresh Swathi , Vellapandian Chitra 
Published: January 2024

Background:
Bisphenol A (BPA) is a known neurotoxic compound with potentially harmful effects on the nervous system. Cyanidin (CYN) has shown promise as a neuroprotective agent.

Objective:
The current study aims to determine the efficacy of CYN against BPA-induced neuropathology.

Methods:
In vitro experiments utilized PC12 cells were pre-treated with gradient doses of CYN and
further stimulated with 10ng/ml of BPA. DPPH radical scavenging activity, catalase activity, total
ROS activity, and nitric oxide radical scavenging activity were done. In vivo assessments employed
doublecortin immunohistochemistry of the brain in BPA-exposed Sprague-Dawley rats. Further, in
silico molecular docking of CYN with all proteins involved in canonical Wnt signaling was performed using the Autodock v4.2 tool and BIOVIA Discovery Studio Visualizer.

Results:
IC50 values of CYN and ascorbic acid were determined using dose-response curves, and it
was found to be 24.68 ± 0.563µg/ml and 20.69 ± 1.591µg/ml, respectively. BPA-stimulated cells pretreated with CYN showed comparable catalase activity with cells pre-treated with ascorbic acid
(p=0.0287). The reactive species production by CYN-treated cells was significantly decreased compared to BPA-stimulated cells (p<0.0001). Moreover, CYN significantly inhibited nitric oxide production compared to BPA stimulated and the control cells (p<0.0001). In vivo, CYN positively affected immature neuron quantity, correlating with dosage. During molecular docking analysis, CYN
exhibited a binding affinity > -7Kcal/mol with all the key proteins associated with the Wnt/β-catenin
signaling cascade.

Conclusion:
Conclusively, our finding suggests that CYN exhibited promise in counteracting BPAinduced oxidative stress, improving compromised neurogenesis in hippocampal and cortical regions,
and displaying notable interactions with Wnt signaling proteins. Thereby, CYN could render its neuroprotective potential against BPA-induced neuropathology
Nanostructured Iron (III)-Copper (II) Binary Oxide as a Highly Efficient
Magnetically Recoverable Nanocatalyst for Facile One-pot Synthesis of 2,
4, 5-trisubstituted Imidazole and 1, 4-dihydro Pyridine Derivatives under
Solvent-free Conditions
Authors: Gaikwad Dhananjay N. , Gaikwad Suresh T. , Manjul Rajesh K. , Rajbhoj Anjali S. , Suryavanshi Dayanand M. 
Published: January 2024

Abstract:
The Fe (III)-Cu (II) binary oxide magnetic nanocatalyst emerges as an environmentally
friendly and highly efficient solid acid catalyst, demonstrating remarkable utility in the one-pot
synthesis of 2, 4, 5-trisubstituted imidazole and 1,4-dihydropyridine compounds, all achieved under
solvent-free conditions. A facile co-precipitation method was used to synthesize nanostructured Fe-Cu
binary oxide. Notably, this Fe-Cu binary oxide magnetic nanocatalyst proves its eco-friendly
credentials as an exceptionally efficient and reusable catalyst, offering ease of handling, recovery, and
multiple uses with minimal reactivity loss. Furthermore, the Fe (III)-Cu (II) binary oxide magnetic
nanocatalyst's magnetic separability enhances its practicality, allowing for effortless catalyst retrieval
after reactions. Significantly, the structural characteristics are meticulously elucidated through
advanced analytical techniques, including 1H and 13C nuclear magnetic resonance (NMR)
spectroscopy. This work presents a versatile and sustainable solution for catalysis, with wide-reaching
implications for green chemistry and the development of reusable, efficient catalysts for organic
synthesis. The exceptional performance and eco-friendliness of the Fe-Cu binary oxide magnetic
nanocatalyst underscore its practical significance. Fe-Cu binary oxide magnetic nanocatalyst exhibits
the highest catalytic activity compared to others. The employment of this catalyst consistently delivers
excellent yields in the target reactions, highlighting its potential to contribute positively to sustainable
chemical processes.
Hypoparathyroidism: Musculoskeletal Manifestations Related to
Parathormone Deficiency
Authors: Slouma Maroua , Hannech Emna , Gharsallah Imen 
Published: January 2024

Background:
Hypoparathyroidism is a rare metabolic disorder that can be responsible
for musculoskeletal manifestations.

Aim:
We present a systematic review of musculoskeletal manifestations of adult-onset nonsurgical
nongenetic hypoparathyroidism.

Methods:
A systematic review was performed according to the Preferred Reporting Items for Systematic
Reviews and Meta-Analyses guideline using the MEDLINE database, including
manuscripts describing musculoskeletal manifestations of adult-onset nonsurgical nongenetic hypoparathyroidism.

Result:
Musculoskeletal manifestations included myopathy, shoulder disorder, immune-negative
non-erosive peripheral arthritis, axial involvement simulating spondylarthritis, and diffuse ligamentous
ossifications. An association between hypoparathyroidism and spondyloarthritis or autoimmune
diseases is possible. T-cell activation, seen in patients with hypoparathyroidism, may
explain the co-occurrence of hypoparathyroidism with other autoimmune diseases.

:
The treatment of these manifestations is based on calcium and active vitamin D supplementation.
Parathyroid hormone may have an anabolic effect on muscle atrophy and muscle weakness.
Parathyroid hormone can also promote bone formation and bone resorption by stimulating osteoclast
differentiation by increasing RANKL (receptor activator for nuclear factor kappa-B ligand)
expression. Therefore, hypoparathyroidism can be responsible for an increase in bone mineral density.
However, the risk of fractures does not appear to be reduced due to changes in bone microarchitecture
and the high risk of falls. Treatment with parathyroid hormone has been shown to improve
bone microarchitecture.

Conclusion:
Our review showed that musculoskeletal manifestations are frequent in patients with
hypoparathyroidism, including muscular, axial, peripheral articular, and entheseal manifestations.
Journey of Teplizumab: A Promising Drug in the Treatment of Type 1 Diabetes Mellitus
Authors: Sharma Nikita , Das Debashree Debasish , Chawla Pooja A. 
Published: January 2024

:
Type 1 diabetes (T1D) is a chronic autoimmune disease caused by CD4+ and CD8+
that are activated via CD3+ cells and finally lead to the macrophages destroying the beta cells in
the pancreas thereby causing diabetes. The anti-CD3 humanized monoclonal antibody was approved
on 17th November 2022 by the United States Food Drug Administration (USFDA) with
the name teplizumab and the brand name TZIELD. This is the only approved drug that treats
type 1 diabetes (T1D) by delaying the onset of stage 3 in type 1 diabetes (T1D). This review outlines
essential features of teplizumab including its brief introduction to its mechanism and other
therapies for the treatment and various risks as well as the pharmacokinetics and pharmacodynamics
of this disease and the clinical trial reports for the completed and ongoing therapies.
Cardiac Axis in Early Gestation and Congenital Heart Disease
Authors: Carrasco D. , Guedes-Martins L. 
Published: January 2024

Abstract:
Congenital heart defects represent the most common structural anomalies observed in
the fetal population, and they are often associated with significant morbidity and mortality.

:
The fetal cardiac axis, which indicates the orientation of the heart in relation to the chest wall, is
formed by the angle between the anteroposterior axis of the chest and the interventricular septum
of the heart. Studies conducted during the first trimester have demonstrated promising outcomes
with respect to the applicability of cardiac axis measurement in fetuses with congenital heart defects
as well as fetuses with extracardiac and chromosomal anomalies, which may result in improved
health outcomes and reduced healthcare costs.

:
The main aim of this review article was to highlight the cardiac axis as a reliable and powerful
marker for the detection of congenital heart defects during early gestation, including defects that
would otherwise remain undetectable through the conventional four-chamber view.
Isocratic RP-HPLC Method Development, Validation, and Optimization
of BCS-II in Bulk and Dosage Form
Authors: Handa Uditi , Malik Anuj , Guarve Kumar , Jan Fatimah , Nagpal Kajal 
Published: January 2024

Background::
Previous studies of dextromethorphan hydrobromide basically worked
on simultaneous research with other compounds. So, the development of a novel method using
the isocratic elution mode is needed.

Objective::
For the detection of dextromethorphan hydrobromide (DXM) in diverse matrices, a
straightforward, accurate, and sensitive reversed-phase HPLC technique using a Waters 2487
Dual λ Absorbance detector has been designed and validated.

Methods:
In this experimental work, utilizing methanol/pH 3.0 potassium dihydrogen phosphate
buffer (70:30, v/v) as the mobile phase, the separation was completed in 7 minutes on a C-18
HPLC column (4.6 cm length, 4.6 mm internal diameter; 5 μm particle size) utilizing an isocratic
elution mode, flow rate of 1.0 mL/min, and UV-detection at 278 nm. Integration of the
chromatography response was carried out using Empower 2.4 software

Results::
With an R2 of 0.9987, the current approach showed high linearity for DXM in the 10-
60 ppm range (retention time 4.281 ± 0.505 min). For DXM Hbr, the limits of detection (LOD
& LOQ) were 10.633 μg/mL and 32.221μg/mL, respectively. Samples remained stable in the
presence of the matrices without any apparent influence.

Conclusion::
The novel approach, which used a straightforward liquid/liquid extraction procedure
with recovery ranging from 100 ± 10 % performed by two different analytes, was accurate.
The precision within and between days was ≤ 2.0% (RSD). The technique was proven to
be reliable and repeatable, and it can be utilized with pharmacological (active ingredients, syrups)
and also for biological (blood) matrices which can be used in future research work for bioanalytical
method development such as pharmacokinetics studies.
Formulation, Analytical Method Development and Validation of an
Emulsion of Multi-enzyme with Carminative Oils
Authors: Kabiraj Amitava , Deshmukh Rohitas 
Published: January 2024

Introduction::
Indigestion leading to Flatulence is a common problem for infants, and
tackling it is tedious for the parents. So, addressing the issue with an ideal formulation should likely
have a combination of digestive enzymes and carminatives.

Methods:
A formulation containing enzymes like Fungal Diastase (Amylase) and Papain (Protease)
for the digestion of Carbohydrates and Protein, respectively, along with aromatic, volatile, carminative
oils like Dill Oil, Anise Oil, and Caraway Oil can serve the purpose to mitigate problems associated
with infant indigestion and flatulence with maximum compliance.

Results:
The stability of multi-enzyme and analysis of carminative oil mixtures still need to be improved
due to their inherent characteristics. Enzymes are very likely susceptible to changes in temperature
and pH, while the solubility of carminative oils is minimal in the aqueous phase. Also,
each enzyme is stable in different pH ranges. Nine emulsions were developed using a suitable buffer
system and analyzed by HPLC method.

Conclusion::
The optimum pH range was found, and analytical method validation was done for the
method's accuracy, precision, and repeatability. The optimum pH was 6-6.5, and the active pharmaceutical
ingredients (API) assay was found within the acceptable limit of NLT, 90% for enzymes
and 90-110% for carminative oils.
Emerging Approaches for the Treatment of Rheumatoid Arthritis: An Outlook
Authors: Kumari Priyanka , Jain Sanjay K. , Saraf Shivani , Tiwari Ankita , Panda Pritish , Verma Amit 
Published: January 2024

Background:
Rheumatoid arthritis (RA) is an inflammatory disease that causes pannus
formation, thickened synovium, joint bone reabsorption, and acute impairment, and increases
the death rate. Many people with RA now live better lives as a result of recent improvements
in treatment, which have dramatically slowed the disease's course. However, a significant
portion of patients continue to either be non-responsive to existing treatments or have
developed a resistance to them. Nanotechnology is becoming a more and more intriguing tool
for investigating novel strategies, ranging from treating various disease states to tackling complicated
conditions.

Objective:
The primary goal of the work was to outline the research activities on versatile
nanocarriers, like polymeric micelles, nanoparticles, liposomes, etc., with controlled/sustained
drug release patterns fabricated to elevate the effectiveness of drug delivery.

Methods:
This review mainly focuses on emerging strategies to deliver various nanocarriers
encapsulating anti-rheumatic drugs, enzymes, genes, phytoconstituents, etc. It also includes upto-
date progress regarding patents and clinical trials filed for the treatment of RA.

Results:
In most of the recent studies, nanocarrier-based drug delivery has gained attention
worldwide and led to the development of new approaches for treating RA. A better understanding
of pathophysiology and signalling pathways helps to select the antirheumatic drug. The encapsulation
of active moiety into the novel nanocarrier enhances the solubility of insoluble
drugs. It restricts the exposure of the drug to the non-inflamed site using various targeting strategies,
like active, passive, or biomimetic targeting and stimuli-responsive carrier systems to enhance
the drug delivery mechanism.

Conclusion:
A brief description of current RA treatments using nanocarrier technology is provided
in this paper, along with predictions for potential enhancements to the nanotherapeutic
regimen.
Prospects of Identifying Alternative Splicing Events from Single-Cell RNA Sequencing Data
Authors: Wang Jiacheng , Yuan Lei 
Published: January 2024

Background:
The advent of single-cell RNA sequencing (scRNA-seq) technology has
offered unprecedented opportunities to unravel cellular heterogeneity and functions. Yet, despite
its success in unraveling gene expression heterogeneity, accurately identifying and interpreting
alternative splicing events from scRNA-seq data remains a formidable challenge. With advancing
technology and algorithmic innovations, the prospect of accurately identifying alternative splicing
events from scRNA-seq data is becoming increasingly promising.

Objective:
This perspective aims to uncover the intricacies of splicing at the single-cell level and
their potential implications for health and disease. It seeks to harness scRNA-seq's transformative
power in revealing cell-specific alternative splicing dynamics and aims to propel our understanding
of gene regulation within individual cells to new heights.

Methods:
The perspective grounds its method on recent literature along with the experimental
protocols of single-cell RNA-seq and methods to identify and quantify the alternative splicing
events from scRNA-seq data.

Results:
This perspective outlines the promising potential, challenges, and methodologies for leveraging
different scRNA-seq technologies to identify and study alternative splicing events, with a
focus on advancing our understanding of gene regulation at the single-cell level.

Conclusion:
This perspective explores the prospects of utilizing scRNA-seq data to identify and
study alternative splicing events, highlighting their potential, challenges, methodologies, biological
insights, and future directions.
Dog Bite Injury: Rusk Became a Risk - A Case Report
Authors: Nehru Sadasivam , Thangaraju Pugazhenthan , Cibee Nehru , Velmurugan Hemasri 
Published: January 2024

Background:
Dog bites are a worldwide problem that disproportionately affects
children. It might be difficult to keep in mind that even a man's closest buddy can be dangerous
to people. In light of the significant morbidity and mortality associated with dog bites, we report, herein, an intriguing case in which a two-year-old boy appeared with eyelid facial lacerations as a result of a dog bite.

Case Presentation:
A two-year-old boy presented with bleeding around the right and left eyes.
Local examination of the eyes revealed laceration and disfiguration of the left orbit and abrasion
around the right eye. Preoperatively, parenteral antibiotics were started and continued postoperatively. Dressing was done for the right eye. Lid reconstruction surgery for the left eye was
scheduled and it was carried out under general anaesthesia. The child was monitored for a
month and showed satisfactory wound healing with no visible scars or complications.

Discussion:
The standard treatment for bites is direct suture, grafting, or local flaps, depending
on the type of wound and the surgeon's preference, regardless of the time since the attack.
Crush wounds are difficult to treat because the damage to the tissues is often so profound that
amputation is unavoidable.

Conclusion:
The global injuries caused by dog bites are the consequence of uncommon illnesses and often manifest as peri-orbital and ocular lesions. Most rabies cases occur in underserved
rural and urban areas, with a high frequency of stray dogs without immunisation.
Promising Influences of Moringa oleifera in Functional Foods against
Metabolic Syndrome: A Comprehensive and Mechanistic Review
Authors: Masoumvand Mohammad , Ramezani Elmira , Rahimi Vafa Baradaran , Askari Vahid Reza 
Published: January 2024

:
Metabolic syndrome (MetS) is now considered a global issue with a growing financial
and health impact. Numerous herbal alternatives have been examined and researched due to
the ever-increasing demand for new medications to treat metabolic syndrome disorders. People
have empirically employed Moringa oleifera (MO), a native plant to several Asian nations, for a
variety of diseases. We sought to examine recent research on MO in MetS and its potential
mechanism of action in the current review. Four databases, including PubMed, Scopus, Web of
Sciences, and Google Scholar, were thoroughly searched, and the data were then compiled. In
total, 146 papers covering nonclinical and clinical MO investigations in metabolic syndromerelated
disorders are included in this study. Numerous research confirmed MO's positive impact
on the control of blood glucose, blood pressure, hyperlipidemia, and obesity. Many molecular
processes have been investigated, including increasing glucose transporter type 4 (GLUT4) expression,
inhibition of β-Hydroxy-β-methylglutaryl-coenzyme A (HMG-CoA), α-glucosidase
inhibiting, AMP-activated protein kinase (AMPK) activation, and other suggested mechanisms.
The current review established much data favoring MO's potential advantages in metabolic syndrome.
However, further research involving human studies is required in this area to determine
whether Moringa can effectively treat metabolic syndrome.
D-Optimal Mixture Design Enabled Development of Lyophilized
Nanoemulsifying Drug Delivery System of Paliperidone
Authors: Das Prativa , Panda Jyanaranjan , Panigrahi Kahnu Charan , Patra Chinam Niranjan , Jena Goutam Kumar 
Published: January 2024

Background:
Schizophrenia is a chronic disease with acute psychotic symptoms, which is having frequent recurrence. Paliperidone palmitate (PP) is a second-generation antipsy-chotic drug to treat schizophrenia.

Aims:
The aim of the study was to prepare lyophilized nanoemulsifying drug delivery system (NEDDS) of paliperidone (PD).

Objective:
The primary objective of the current research work was to develop a lyophilized nanoemulsifying drug delivery system (NEDDS) of paliperidone (PD) to improve its oral bioa-vailability and stability.

Methods:
Optimization using D-Optimal Mixture Design DMD) was conducted, and optimized NEDDS was further lyophilized to improve stability. The lyophilized optimized NEDDS was fur-ther evaluated for biopharmaceutical evaluation.

Result:
A saturation solubility study revealed Peceol, Tween 80, and Plurol Olique CC497 as suitable candidates for oil, surfactant, and co-surfactant, respectively. Optimized NEDDS of PD showed mean globule size (MGS) of 185 nm, PDI of 0.27 and cumulative % drug release within 15 min Q15 of 86.6%. Lyophilized optimized NEDDS was found to have no significant change in quality attributes within the stability study period. A pharmacokinetic study revealed more than two-fold increases in bioavailability for lyophilized optimized NEDDS.

Conclusion:
Hence, lyophilized NEDDS of PD can be used as an effective approach for the im-provement of oral bioavailability and stability.
Lipid-based Nanocarriers for mRNA Delivery: Vital Considerations and
Applications
Authors: Sharma Teenu , Babu M Arockia , Jain Atul , Sharma Deepika 
Published: January 2024

Abstract:
The use of mRNA in therapeutics has lately emerged as a powerful strategy for alleviating
the various viral infections and diseased conditions, along with prophylaxis. However, a key challenge
in their efficient delivery is the protection of the nucleic acid from degradation followed by
mRNA transport to the cells. In this regard, clinical translation of mRNA therapeutics has largely
been facilitated with the advent of lipid-based nanoparticles (LBNPs). LBNPs–mRNA vaccines currently
being employed for Covid 19 is one such instance substantiating and endorsing the use of
lipidic nanocarriers for mRNA therapeutics. Thus, the current review article aims to furnish information
on developmental challenges, different aspects of lipid-based carrier systems for mRNA delivery,
their vital applications in different diseases and the future potential of LBNPs in therapeutics.