bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of lipid extraction and room temperature transportation of bovine oocytes determined by MRM profiling
Authors: de Lima Camila Bruna , Milazzotto Marcella Pecora , Vireque Alessandra Aparecida , Joaquim Daniel Carlino , Sobreira Tiago Jose Paschoal , Ferreira Christina Ramires 
Published: December 2023

Abstract

Lipids play many important physiological roles in mammalian reproduction, being essential for the acquisition of oocyte competence and post-fertilization embryonic development. Lipid profiling in samples of minute size, such as oocytes, is challenging but has been achieved by mass spectrometry technologies such as multiple reaction monitoring (MRM) profiling. With the goals of further simplifying sample workflow and investigating the influence of pre-analytical conditions, we have evaluated how different extraction methods and transportation of lipid extracts in vacuum and at room temperature impacted the lipid profile of bovine oocytes. Using a comprehensive method, 316 MRMs associated with lipids of 10 different classes were screened in oocyte lipid extracts prepared by 2 extraction methods (one-step methanol addition or Bligh and Dyer) and transporting them in dry ice or at room temperature inside vacuum packages. No changes in the multivariate analysis (PCA) were noticeable due to transportation temperature, while lipid profiles were more affected by the lipid extraction protocol. Sample extraction using pure methanol favored the detection of phospholipids uniformly, while Bligh and Dyer favored the detection of neutral intracellular lipids. Triacylglycerol lipids and free fatty acids yielded decreased abundances when samples were transported at room temperature. We conclude that if samples are submitted to the same lipid extraction protocol and same transportation batch at room temperature coupled with vacuum conditions it is possible to analyze lipid extracts of bovine oocytes and still obtain informative lipid profiling results.
THE TEACHING PROCESS IN TIMES OF COVID-19: BETWEEN THE POSSIBLE AND THE IDEALIZED
Authors: Carius Ana Carolina , Rodrigues Scartoni Fabiana 
Published: December 2023

This article aims to study the development of so-called remote teaching during the closure of Brazilian schools due to the COVID-19 pandemic. Therefore, a research approach was chosen mixed in a convergent way through a case study of the municipal system of Petropolis education. The main objective of the research is to evaluate the existence (or not) of pedagogical theories, such as new school, technician, or socio-critical pedagogy, that have supported the different pedagogical practices carried out during the school closing period. Through questionnaires and semi-structured interviews, conducting a qualitative study on the topic was possible using Content analysis. It is concluded that the adaptation to remote teaching was made suddenly without preparing teachers, students, managers, and families. Little was reflected about existing pedagogical theories to improve the effectiveness of Education during the school closure period.
Breast Cancer-related Lymphedema: Recent Updates on Clinical Efficacy of
Therapies and Bioengineering Approaches for a Personalized Therapy
Authors: Zhang Xinliang , Beeraka Narasimha M. , Sinelnikov Mikhail Y. , Glazachev Oleg S. , Ternovoy Konstantin S. , Lu Pengwei , Isaeva Aida , Cao Yu , Zhang Jin , Nezhad Arshia Bakhtiari , Plotnikova Maria , Chen Kuo 
Published: December 2023

Background:
Post-mastectomy lymphedema is a chronic progressive disease characterized by a significant
reduction in quality of life and a range of complications.

Aim:
To this date, no single treatment method provides pathological correction of the mechanisms associated
with tissue reorganization observed in later-stage breast cancer-related lymphedema (BCRL).

Methods:
To define a personalized approach to the management of patients with iatrogenic lymphedema, we
performed a systematic review of literature without a comprehensive meta-analysis to outline existing molecular-
genetic patterns, overview current treatment methods and their efficacy, and highlight the specific tissue-associated
changes in BCRL conditions and other bio-engineering approaches to develop personalized therapy.

Results:
Our results show that several tissue-specific and pathological molecular markers may be found, yet
current research does not aim to define them.

Conclusion:
As such, currently, a strong foundation for further research into molecular-genetic changes in
lymphedema tissue exists, and further research should focus on finding specific targets for personalized treatment
through bio-engineering approaches.
Privileged Scaffolds in Drug Discovery against Human Epidermal Growth Factor
Receptor 2 for Cancer Treatment
Authors: Peerzada Mudasir Nabi , Hamdy Rania , Rizvi Masood Ahmad , Verma Saurabh 
Published: December 2023

Abstract:
HER2 is the membrane receptor tyrosine kinase showing overexpression in several human malignancies,
particularly breast cancer. HER2 overexpression causes the activation of Ras- MAPK and PI3K/Akt/
NF-κB cellular signal transduction pathways that lead to cancer development and progression. HER2 is, therefore,
presumed as one of the key targets for the development of tumor-specific therapies. Several preclinical
have been developed that function by inhibiting the HER2 tyrosine kinase activity through the prevention of
the dimerization process. Most HER2 inhibitors act as ATP competitors and prevent the process of phosphorylation,
and abort the cell cycle progression and proliferation. In this review, the clinical drug candidates and
potent pre-clinical newly developed molecules are described, and the core chemical scaffolds typically
responsible for anti-HER2 activity are deciphered. In addition, the monoclonal antibodies that are either used
in monotherapy or in combination therapy against HER2-positive cancer are briefly described. The identified
key moieties in this study could result in the discovery of more effective HER2-targeted anticancer drug molecules
and circumvent the development of resistance by HER2-specific chemotherapeutics in the future.
Efficient Synthesis and Characterization of 6-substituted-N-p-tolyl-imidazo[1,2-
a]pyridine-8-carboxamide: A Promising Scaffold for Drug Development
Authors: Bhatt Keval , Patel Dhara , Rathod Mrudangsinh , Patel Ashish , Shah Drashti 
Published: December 2023

Abstract:
Imidazo[1,2-a]pyridine is a highly significant fused bicyclic heterocycle widely
utilized in medicinal chemistry, and it holds a prominent position as a "drug prejudice"
framework because of its extensive applications. Numerous approaches have been documented
for the synthesis of imidazo[1,2-a]pyridines, with a particular focus on functionalizing
these compounds. In this research, we have presented an effective multi-step synthesis method
for producing imidazo[1,2-a]pyridines, achieving impressive yields ranging from 93% to
97%. The synthesized compounds were subjected to thorough characterization using various
spectral analysis techniques, including FTIR, 1H NMR, 13C NMR, mass spectrometry, and
elemental analysis. Overall, this synthetic strategy possesses desirable features for the synthesis
of imidazo[1,2-a]pyridines and their derivatives, including high product purity, accessible
starting materials, broad substrate scope, scalability, and transformability. These characteristics make it a promising
approach for further development and potential application in the synthesis of valuable therapeutic compounds.
Comparative Pharmacokinetics of Intravenous Enrofloxacin in One- Six- And
Twelve-Month-Old Sheep
Authors: Coskun Devran , Corum Orhan , Durna Corum Duygu , Uney Kamil 
Published: December 2023

Background:
Enrofloxacin (ENR) is a fluoroquinolone antibiotic approved for use in sheep of all
ages. The body composition and metabolic capability change with age. These changes may alter the pharmacokinetics
of drugs and thus their effect. Therefore, the pharmacokinetics of drugs need to be established in target-
age animals

Objective:
To determine the pharmacokinetics of ENR and its active metabolite, ciprofloxacin (CIP), following
a single intravenous administration of ENR at a dose of 10 mg/kg in different ages of sheep.

Methods:
The study was carried out in the one-, six- and twelve-month age period of the sheep. A single dose
of 10 mg/kg ENR was administered intravenously through the jugular vein to sheep in all age periods. ENR
and CIP plasma concentrations were determined using HPLC–UV and analyzed using a non-compartmental
method.

Results:
ENR was detected in the plasma until 36 h in one-month-old and up to 24 h in other ages. CIP was detected
in the plasma up to 24 h in all age groups. The t1/2ʎz and Vdss were significantly higher in one-month-old
sheep than in six and twelve-months old sheep. There was no difference in ClT and AUC values in different
age groups. AUC0-∞CIP/AUC0-∞ENR ratios were higher in one-month-old than in six- and twelve-months sheep.

Conclusion:
The most important pharmacokinetic changes associated with aging in sheep are decreased Vdss
and t1/2ʎz of ENR and the low ratio metabolizing of ENR to CIP. Pharmacokinetic/pharmacodynamic data
showed that ENR after IV administration of 10 mg/kg dose provided the optimal AUC0–24/MIC90 ratios for E.
coli, P. multocida and Mycoplasma spp. (>125) with MIC of 0.37 μg/mL and for S. aureus (>30) with MIC of
0.5 μg/mL in all ages of sheep.
Does the Grape Products Intake has an Effect on Body Weight in Adults:
A Systematic Review and Meta-analysis of Randomized Controlled Trials
Authors: Goudarzi Mohammad Ali , Sohrabi Zahra , Hashempur Mohammad Hashem , Nosratabadi Saeed , Namkhah Zahra , Clark Cain C.T. , Haghighat Neda 
Published: December 2023

Introduction::
There is a growing interest in the considerable benefits of grape products
intake, as some studies have indicated that they may improve cardiometabolic risk factors. However,
the widespread impact of grape products on the anthropometric indices is not fully resolved.

Method::
The purpose of this systematic review and meta-analysis was to examine the effects of
grape products intake on anthropometric indices in adults. Randomized controlled trials (RCT) examining
the effects of grape products intake on anthropometric indices, published up to December
2021, were identified through PubMed, SCOPUS, and ISI Web of Science databases. 30 studies
with 35 effect sizes, including 1284 participants (708 cases and 576 controls), were included and
analyzed using a random-effects model to calculate weighted mean differences (WMDs) with
95% confidence interval (CI).

Result::
The outcomes have revealed grape products intake to significantly decrease body weight
(p = 0.001) and body mass index (p = 0.004) in obese participants, and also, a greater effect was
observed when grape seed extract was used.

Conclusion::
Our study suggests that grape products intake may help to decrease body weight in
obese participants. Future large RCTs with longer duration and obese populations are needed to expand
our findings.
Predicting Futile Recanalization in Acute Ischemic Stroke Patients
Undergoing Endovascular Thrombectomy: The Role of White Blood Cell
Count to Mean Platelet Volume Ratio
Authors: Yu Wantong , Jia Milan , Guo Wenting , Xu Jiali , Ren Changhong , li Sijie , Zhao Wenbo , Chen Jian , Duan Jiangang , Ma Qingfeng , Song Haiqing , Ji Xunming 
Published: December 2023

Background:
Approximately half of AIS patients have an unfavorable outcome even
after complete reperfusion. White blood cell (WBC) count to mean platelet volume (MPV) ratio
(WMR) may be a promising predictive factor for futile recanalization. This study aimed to determine
the predictive value of WMR in identifying individuals at higher risk of futile recanalization.

Methods:
In this retrospective cohort study, 296 patients who achieved complete reperfusion
after endovascular treatment (EVT) were included in the analysis. WBC count and MPV were
collected at admission. Multivariable logistic regression was used to examine the independent
association of the WMR with functional outcomes at three months. Net reclassification improvement
(NRI) and integrated discrimination improvement (IDI) analyses were used to compare
the accuracy of WMR for predicting futile recanalization.

Results:
The adjusted odds ratios for the fourth quartile of WMR were 3.142 (95% CI 1.405-
7.027, P = 0.005) for unfavorable outcomes at 3 months in comparison with the first quartile.
The inclusion of WMR in the traditional model enabled a more accurate prediction of unfavorable
outcomes (NRI 0.250, P = 0.031; IDI 0.022, P = 0.017).

Conclusion:
Elevated WMR at admission was independently associated with futile recanalization
among AIS patients who received EVT and might be useful in identifying futile recanalization.
The Effect of Silencing MiR-4270 on Apoptosis in HCC Cell Line
Authors: Gholamia Hanieh , Akrami Hassan , Sassan Hosseinali , Erfani Nasrollah , Fattahi Mohammad Reza , Heidari Mojdeh 
Published: December 2023

Background::
Hepatocellular carcinoma (HCC) is the most common type of cancer.
Although HCC treatment has greatly improved over the past few decades, patient survival rates
are still very low. Therefore, it is essential to find new treatments for HCC. Apoptosis has been
shown to be the most effective in disrupting cancer growth. Improper functioning of proteins in
apoptosis can lead to cancer growth. MicroRNAs (miRNAs) are key regulators in the development
and progression of HCC.

Objective::
Irregular expression of miRNAs involved in apoptosis signaling can lead to tumorigenesis.
Therefore, we investigated the effect of the hsa-miR-4270 inhibitor on cell proliferation
and apoptosis.

Methods::
HepG2 cells were cultured at 37°C and 95% air. Transfection of HepG2 cells was
performed by miR-4270 inhibitor and lipofectamine 2000. Cell proliferation of HepG2 cells was
determined with MTT assay and different concentrations of miR-4270 specific inhibitors. DNA
laddering assay was performed to evaluate the induction of apoptosis. Finally, the transcription
level of genes involved in apoptosis, including BAX, BCL2, Caspase3, and p53, was measured
by real-time RT-PCR.

Results::
The results of MTT and DNA laddering assays showed that the miR-4270 inhibitor
declined cell proliferation and induced apoptosis in HepG2 cells. Also, the results of quantitative
real-time RT-PCR indicated an upregulation of transcription of BAX, p53 and Caspase3 genes
and a decline in expression of BCL2 gene.

Conclusion::
Taken together, we found hsa-miR-4270 inhibitor decreased cell proliferation and
induced apoptosis in the HepG2 cell line, which can be used as a new therapeutic strategy for
HCC patients.
BERT-Based Neural Network for Inpatient Fall Detection From Electronic Medical Records: Retrospective Cohort Study
Authors: Cheligeer Cheligeer , Wu Guosong , Lee Seungwon , Pan Jie , Southern Danielle A , Martin Elliot A , Sapiro Natalie , Eastwood Cathy A , Quan Hude , Xu Yuan 
Published: December 2023

Background
Inpatient falls are a substantial concern for health care providers and are associated with negative outcomes for patients. Automated detection of falls using machine learning (ML) algorithms may aid in improving patient safety and reducing the occurrence of falls.

Objective
This study aims to develop and evaluate an ML algorithm for inpatient fall detection using multidisciplinary progress record notes and a pretrained Bidirectional Encoder Representation from Transformers (BERT) language model.

Methods
A cohort of 4323 adult patients admitted to 3 acute care hospitals in Calgary, Alberta, Canada from 2016 to 2021 were randomly sampled. Trained reviewers determined falls from patient charts, which were linked to electronic medical records and administrative data. The BERT-based language model was pretrained on clinical notes, and a fall detection algorithm was developed based on a neural network binary classification architecture.

Results
To address various use scenarios, we developed 3 different Alberta hospital notes-specific BERT models: a high sensitivity model (sensitivity 97.7, IQR 87.7-99.9), a high positive predictive value model (positive predictive value 85.7, IQR 57.2-98.2), and the high F1-score model (F1=64.4). Our proposed method outperformed 3 classical ML algorithms and an International Classification of Diseases code–based algorithm for fall detection, showing its potential for improved performance in diverse clinical settings.

Conclusions
The developed algorithm provides an automated and accurate method for inpatient fall detection using multidisciplinary progress record notes and a pretrained BERT language model. This method could be implemented in clinical practice to improve patient safety and reduce the occurrence of falls in hospitals.
INFLUENCE OF CUSTOMER EXPERIENCE ON INTEREST USING THE TIX.ID APPLICATION IN PURCHASING CINEMA TICKETS IN JAMBI CITY WITH CUSTOMER SATISFACTION AS AN INTERVENING VARIABLE
Authors: Subhi Ahmad , Erida Erida , Yuniarti Yenny 
Published: December 2023

The perpetrator's business can use the internet and digital marketing as a medium for support activities marketing , like For do promotion, buying and selling process. One of them is the emergence of online sites and applications internet-based _ used to help do activity sales or purchase products online. Study This is study descriptive quantitative aim _ To explain the influence Customer Experience and Customer Satisfaction as well as interest_use repeat consumers who have application Tix. Id . Population in research This is user application Tix.Id in Jambi City with sample as many as 75 respondents . Withdrawal sample use non-probability sampling with the technique purposive sampling . Data collection using google form. Deep data analysis techniques study This is Partial Least Square use application SmartPLS . Research results This show that : Customer Experience and Customer Satisfaction influential positive and significant to Interest Use Repeat , Customer Experience influential positive and significant to Customer Satisfaction. CustomerExperience influence interest use repeat through customer satisfaction as intervening variable
TAFSIR MAQASIDI OF SURA AL-FURQAN (25): 74 IN RELATION TO THE EMERGENCE OF THE KID INFLUENCER
Authors: Hidayat M. Riyan , Munshihah Aty , Almujahid Almujahid , Faizah Thoriqotul 
Published: December 2023

It is normal for the popularity of the parents to affect the popularity of the child in the age of information and communication technologies. However, many Indonesian popular personalities actually purposefully incorporate their kids in their job because the kids have many admirers who genuinely appreciate them. Whether we are aware of it or not, the digital era has an influence on the term influencer’s rise in a range of age groups, including youngsters. Influencers play a common role in the digital world, but influencers are also held in high regard by young people (kids). The use of Kid influencers frequently serves as a means of subsistence and promotes the use of child exploitation. In order to analyze sura Al-Furqan (25): 74 and find maqasid and moral notions, this study uses library research and Tafsir Maqasidi. Tafsir Maqasidi is used since it falls under the contemporary category and has applications for solving current issues. According to this study, sura Al-Furqan (25):74 is not merely a lofty ideal; it must be put into practice by parents in a variety of roles toward their children. This verse also contains maqasid aspects that lead to hifs nafz and hifz nasl.
FROM READING THE QUR’AN TO LEARNING ARABIC LANGUAGE
Authors: Yunita Yunita 
Published: December 2023

This article discusses the Qur’anic verses (Q. 12: 2, Q. 13: 37, Q. 20: 113, Q. 26: 195, Q. 39: 28, Q. 41: 3, Q. 41: 44, Q. 42: 7, Q. 43: 3, Q. 46: 12) that talk about the nature of the Qur’an as an Arabic text (kitab ʿArabī), and how two Indonesian mufassirs (Hamka and Quraish Shihab) use them to urge readers to learn Arabic. Their works are chosen because they are the two most prominent Qur’anic commentators of the country. This qualitative research concludes that the two mufassirs employ variety of strategies to raise the status of the Arabic language and encourage readers to learn them, including (1) a historical trajectory to the biography of the Prophet Muhammad, (2) an emphasis on the universality of the Prophet’s missionary, (3) on the privileges of non-Arabs (al-ʿajam) who master the Arabic language, and (4) on the uniqueness and peculiarities that the language possesses in comparison to other languages.
MENIMBANG KASUS PERNIKAHAN ANAK DALAM KACAMATA TAFSIR MAQĀṢIDĪ
Authors: Fazlin Hani 
Published: December 2023

Tulisan ini berupaya untuk melakukan tinjauan ulang terhadap konsep “pernikahan” yang terdapat dalam Al-Qur’an sebagai sumber otoritatif dalam Islam. Penelitian ini menggunakan pendekatan Tafsir Maqāṣidī sebagai salah satu upaya dalam merekonstruksi konsep pernikahan. Adapun objek material utama dalam penelitian ini yaitu surah al-Rūm/30: 21 terkait konsep umum pernikahan dan beberapa ayat pendukung lainnya. Setelah dilakukan analisis mendalam dengan kacamata Tafsir Maqāṣidī , maka ditemukan aspek-aspek fundamental maqāṣid dari ayat-ayat pernikahan; ḥifẓ al-dīn, ḥifẓ al-nafs, ḥifẓ al-‘aql, ḥifẓ al-māl, ḥifẓ al-nasl, dan ḥifẓ al-daulah. Kemudian berdasarkan analisis aspek maqāṣid AlQur’an, pernikahan terdiri dari unsur iṣlāḥ al-fard (kemaslahatan individu), iṣlāḥ al-mujtama’ (kemaslahatan sosial), dan iṣlāḥ al-‘ālam (kemaslahatan universal). Dengan demikian, maka disimpulkan bahwa pernikahan anak membawa banyak dampak negatif (mudarat) sehingga praktik ini benar-benar seharusnya dapat dicegah dan dihentikan. Pencegahan ini membutuhkan dukungan dari semua lapisan masyarakat, termasuk pemerintah. Salah satunya dengan cara meninjau ulang terhadap adanya pemberlakuan dispensasi nikah bagi anak-anak yang belum mencapai batas minimal 19 tahun.