bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical options for the analysis of in vitro gas production profiles illustrated using rumen liquor as the inoculum
Authors: Dhanoa M.S. , Sanderson R. , Lister S.J. , Mauricio R.M. , López S. , Ellis J.L. , Powell C.D. , France J. 
Published: November 2023

AbstractThe use of repeated measures analysis of variance (ANOVA) options for the analysis of in vitro ruminal fermentation gas production profiles is illustrated. Because of the different variances and covariance structures among profile observations, ordinary ANOVA for more than two-time points is not recommended. To mitigate this problem, the Greenhouse–Geisser epsilon correction can be applied to reduce the degrees of freedom, inflated by violation of the sphericity assumption, for F ratio probability calculations. After this correction, the Box–Greenhouse–Geisser ANOVA (modified ANOVA) layout appears similar to the layout of a split-plot design ANOVA with whole plots divided into subplots (incubation time). Any F tests in the main plot part are valid but F tests involving the time factor from the subplot part need modification because time factor, by its very nature, cannot be allocated at random. Application of multivariate ANOVA, distance multivariate ANOVA, ante-dependence and mixed model analysis are also considered. All these options lend themselves to wide application in the applied biological sciences.
Immunity mediates host specificity in the human hookworm Ancylostoma ceylanicum
Authors: Langeland Andrea , McKean Elise L. , O'Halloran Damien M. , Hawdon John M. 
Published: November 2023

AbstractHookworm infection affects millions globally, leading to chronic conditions like malnutrition and anaemia. Among the hookworm species, Ancylostoma ceylanicum stands out as a generalist, capable of infecting various hosts, including humans, cats, dogs and hamsters. Surprisingly, it cannot establish in mice, despite their close phylogenetic relationship to hamsters. The present study investigated the development of A. ceylanicum in immunodeficient NSG mice to determine the contribution of the immune system to host restriction. The infections became patent on day 19 post-infection (PI) and exhibited elevated egg production which lasted for at least 160 days PI. Infective A. ceylanicum larvae reared from eggs released by infected NSG mice were infectious to hamsters and capable of reproduction, indicating that the adults in the NSG mice were producing viable offspring. In contrast, A. ceylanicum showed limited development in outbred Swiss Webster mice. Furthermore, the closely related canine hookworm Ancylostoma caninum was unable to infect and develop in NSG mice, indicating that different mechanisms may determine host specificity even in closely related species. This is the first report of any hookworm species completing its life cycle in a mouse and implicate the immune system in determining host specificity in A. ceylanicum.
Thamnolecania yunusii(Ramalinaceae) – A new species of lichenised fungus from Horseshoe Island (Antarctic Peninsula)
Authors: Halici Mehmet Gökhan , Güllü Mithat , Bölükbaşi Ekrem , Kahraman Yiğit Merve 
Published: November 2023

AbstractThe new terricolous lichen speciesThamnolecania yunusiiHalıcı, Güllü, Bölükbaşı & Kahraman, which is characterised by its cream to greyish brown granulose-crustose thallus without vegetative propagules, is described from Horseshoe Island in the South-West Antarctic Peninsula region. AllThamnolecaniaspecies are known only from the Antarctic. The only species of the genus with a crustose thallus isT.racovitzae, but it differs fromT.yunusiiby growing on rocks, having an effuse to subeffigurate thallus that is sometimes isidiate and with shorter and narrower ascospores (c.15 × 3.5 µmvs.15.5–19.5 × 3.5–5.5 µm). The nrITS, mtSSU and RPB1 gene regions of the new species were studied and the phylogenetic position of the species was shown to be in the same clade asThamnolecania gerlachei,T.brialmontiiandT.racovitzae, but occurs on a different branch from these species. AsT.yunusiiis an Antarctic endemic, like the otherThamnolecaniaspecies, and most of the morphological characters fit well with this genus, we describe this new species under the genusThamnolecania.
Clinical and genetic characteristics of catecholaminergic polymorphic ventricular tachycardia combined with left ventricular non-compaction
Authors: Xu Bihe , Yang Jing , Liu Fang , Lv Tingting , Li Kun , Yuan Yifang , Li Siyuan , Liu Yuanwei , Zhang Ping 
Published: November 2023

AbstractBackground:Catecholaminergic polymorphic ventricular tachycardia is an ion channelopathy, caused by mutations in genes coding for calcium-handling proteins. It can coexist with left ventricular non-compaction. We aim to investigate the clinical and genetic characteristics of this co-phenotype.Methods:Medical records of 24 patients diagnosed with catecholaminergic polymorphic ventricular tachycardia in two Chinese hospitals between September, 2005, and January, 2020, were retrospectively reviewed. We evaluated their clinical and genetic characteristics, including basic demographic data, electrocardiogram parameters, medications and survival during follow-up, and their gene mutations. We did structural analysis for a novel variant ryanodine receptor 2-E4005V.Results:The patients included 19 with catecholaminergic polymorphic ventricular tachycardia mono-phenotype and 5 catecholaminergic polymorphic ventricular tachycardia-left ventricular non-compaction overlap patients. The median age of onset symptoms was 9.0 (8.0,13.5) years. Most patients (91.7%) had cardiac symptoms, and 50% had a family history of syncope. Overlap patients had lower peak heart rate and threshold heart rate for ventricular tachycardia and ventricular premature beat during the exercise stress test (p < 0.05). Sudden cardiac death risk may be higher in overlap patients during follow-up. Gene sequencing revealed 1 novel ryanodine receptor 2 missense mutation E4005V and 1 mutation previously unreported in catecholaminergic polymorphic ventricular tachycardia, but no left ventricular non-compaction-causing mutations were observed. In-silico analysis showed the novel mutation E4005V broke down the interaction between two charged residues.Conclusions:Catecholaminergic polymorphic ventricular tachycardia overlapping with left ventricular non-compaction may lead to ventricular premature beat/ventricular tachycardia during exercise stress test at lower threshold heart rate than catecholaminergic polymorphic ventricular tachycardia alone; it may also indicate a worse prognosis and requires strict follow-up. ryanodine receptor 2 mutations disrupted interactions between residues and may interfere the function of ryanodine receptor 2.
Associations between early infections and childhood cognition in the Newcastle Thousand Families Study birth cohort
Authors: Pennock Erin , Slack Emma L. , Grebby Jess A. , Forster Lara N. , Pearce Mark S. 
Published: November 2023

AbstractChildhood infections have been shown to stunt growth, contribute to malnutrition and reduce cognition in early adulthood. This study aimed to assess relationships between early life infections and childhood cognition at age 11 years in the Newcastle Thousand Families Study (NTFS). The analysis included 741 members from the NTFS who had complete data for infections between birth and 5 years, and the 11-plus examinations. School records from the 11-plus examinations showed cognitive (IQ), English (EQ) and arithmetic (AQ) abilities. Housing conditions, overcrowding, birth order and social class were recorded at birth. Helicobacter pylori seropositivity was measured at age 49–51 years. Multivariable linear regression was used to examine relationships between infections and cognition. The total number of infections in the first 5 years of life was not significantly associated with IQ, EQ or AQ, nor were there significant relationships between cognitive outcomes and most infections. Tonsillitis did display a positive, significant association with IQ after adjustment for confounders (b = 6.43, 95% CI 0.92, 11.94, p = 0.022). Lower respiratory tract infections (LRTIs) showed significant negative relationships with all cognitive outcomes. H. pylori seropositivity at age 50 exhibited negative, significant relationships with EQ (p = 0.014) and AQ (p = 0.024) after adjustment for confounders. Although no significant relationship between overall infections and cognition were found, there were indications that LRTIs and gastrointestinal system infections may limit cognitive development. Given these infections remain prevalent, further research regarding severity and recurrence of infections and how they affect childhood cognition is needed.
Steps towards inclusivity: modifying challenging content, navigating pedagogical materials and initiating student reflection within the Classics classroom
Authors: Peddar David 
Published: November 2023

AbstractAlthough there is plenty of scholarship regarding the concerns of addressing controversial and sensitive subject manner in the Classics classroom, and I have considered these to quite an extent, my own interest in these practices emerged where they matter the most: my own experiences within the classroom. For me, it came to a head with one pupil who demonstrated an active enthusiasm for Latin study and the classical world at large, but was slowly becoming disheartened after several classes, despite not displaying any overt academic challenges to any of the presented material. When I brought this issue up privately with her, she said: ‘Sir, it sucks that this culture and language which I adore, wouldn't value me as much I value it.’ She also expressed regret at choosing Latin, as she felt ‘it seems to be a subject where only boys can succeed.’ It also became apparent that this consensus was common, and shared with several of her friends and peers. This paper describes some of the actions I undertook to address their sensitivities in the Classics classroom.
State‐Dependence of the Equilibrium Climate Sensitivity in a Clear‐Sky GCM
Authors: Henry Matthew , Vallis Geoffrey K. , Lutsko Nicholas J. , Seeley Jacob T. , McKim Brett A. 
Published: November 2023

AbstractThe climate sensitivity peaks around 310 K in a wide variety of climate models, ranging from idealized single column models to fully comprehensive climate models. Here, we increase CO2 using a clear‐sky three‐dimensional atmospheric model with a radiation scheme which maintains accuracy for high CO2 and temperature levels. In contrast, the Equilibrium Climate Sensitivity (ECS) of our model plateaus around 310 K. We show that this is due to the moistening of the subtropical regions caused by a slowdown in atmospheric circulation, which increases the ECS at very high CO2 values. When relative humidity is fixed, the ECS peak is consistent with single column model results. This work does not rule out that clouds or other complex processes impact the ECS in comprehensive climate models. Though the changes in CO2 here are extreme, this study underlines the importance of changes in atmospheric circulation and relative humidity in quantitative assessments of climate sensitivity.
Structure of the Martian Crust Below InSight From Surface Waves and Body Waves Generated by Nearby Meteoroid Impacts
Authors: Drilleau Mélanie , Beucler Éric , Shi Jing , Knapmeyer‐Endrun Brigitte , Garcia Raphaël F. , Ansan Véronique , Kim Doyeon , Lognonné Philippe , Banerdt William B. 
Published: November 2023

AbstractWe measure group velocity dispersion of surface waves generated by two meteoroid impacts on Mars close to the lander of the InSight mission. This allows us to probe the crustal structure in the first few kilometers beneath the InSight lander. In combination with body wave arrival times from five impact events, we obtain direct seismic constraints on the seismic velocity of the crust in the vicinity of the InSight landing site. We confirm the existence of a uppermost low‐velocity layer with a mean thickness of ∼1.2 km, interpreted as layered volcanic materials, possibly interstratified with sedimentary and altered materials. Our joint inversion of surface and body waves shows a four‐layer model for the Martian crust, compatible with high‐ and low‐frequency P‐to‐S receiver functions estimated in previous studies.
A North–South Dipole Response of the South Atlantic Convergence Zone During the Mid‐Holocene
Authors: Wong Minn Lin , Battisti David S. , Liu Xiaojuan , Ding Qinghua , Wang Xianfeng 
Published: November 2023

AbstractThe South Atlantic Convergence Zone (SACZ) profoundly modulates precipitation from central to southeastern Brazil in the present‐day climate. However, the understanding of its long‐term behavior responding to various climate forcings remains limited. Here, we use an isotope‐enabled atmospheric general circulation model (ECHAM4.6) to examine the precipitation response of the SACZ during the mid‐Holocene about six thousand years ago. The model simulates a northward intensification of the SACZ in the mid‐Holocene, resulting in a dipole anomaly pattern relative to today's climate. The mid‐Holocene precipitation increased along the northern margin of the SACZ due to the strengthening of easterly winds across the tropical Atlantic, while an eastward deflection of the South American low‐level jet reduced moisture transport to southern Brazil, resulting in reduced precipitation along the southern margins of the SACZ. The north–south dipole response in precipitation is consistent with the mid‐Holocene hydroclimate change observed in proxy records from the region.
Assessing Latent and Kinetic Energy Trend Changes in Extratropical Cyclones From 1940 to 2020: Results From ERA‐5 Reanalysis
Authors: Dzambo Andrew , McFarquhar Greg , Sledd Anne , L’Ecuyer Tristan 
Published: November 2023

AbstractBaroclinic or extratropical cyclones (ETCs) transport heat and moisture to higher latitudes, making it fundamentally important to understand how their influence changes as Earth's climate evolves. A 2–8‐day Lanzcos bandpass filter is applied to European Center for Medium Range Weather Forecasting 5th Generation Reanalysis latent energy (LE) and kinetic energy (KE) data to assess how ETCs have changed from 1940 to 2020 relative to full‐scale changes in LE and KE. Full‐scale KE trends are more positive at high latitudes relative to mid‐latitudes, confirming several previous studies that ETCs have shifted poleward. LE increases have occurred globally, and trends in both full‐scale LE and KE are statistically significant in the southern high latitudes. The high relative fractional contribution of 2–8‐day LE wave power and trend clearly suggest that ETCs have an increasingly important role in poleward moisture transport but are not solely responsible for the observed statistically significant increases.
The Role of Passive Cloud Volumes in the Transition From Shallow to Deep Atmospheric Convection
Authors: Vraciu Cristian V. , Kruse Irene L. , Haerter Jan O. 
Published: November 2023

AbstractProperly capturing the transition from shallow to deep convection remains a major shortcoming of numerical weather and climate models due to poor understanding of the physical processes controlling this transition. Although recent studies suggest shallow preconditioning and cold pool feedbacks to be important, these studies are unable to explain the initial phase of the transition. We identify an additional mechanism, namely the interaction between passive cloud volumes (PCV)—old cumulus cloud volumes in the decaying stage—and updrafts, and discuss the potential role of this mechanism in the transition from shallow to deep convection. We show that the number of updrafts interacting with PCV is very large during the transition and the updrafts better preserve their buoyancy due to entrainment of much moister air. We argue that PCV might play an important role in the transition and cumulus parameterizations could therefore benefit from including them.
The Role of Subslab Low‐Velocity Anomalies Beneath the Nazca Ridge and Iquique Ridge on the Nazca Plate and Their Possible Contribution to the Subduction Angle
Authors: Lee Hwaju , Kim YoungHee , Bezada Maximiliano J. , Clayton Robert W. 
Published: November 2023

AbstractSubducting the buoyant crustal material of an aseismic oceanic ridge has been regarded as a dominant contributor to flat slab subduction. However, normal‐dip subduction is also observed in some cases where ridges are subducting. In this study, we compare the subduction of two ridges on the Nazca Plate: Nazca Ridge (flat slab) and Iquique Ridge (normal‐dip slab). Anisotropy determined by shear wave splitting observation suggests that the low‐velocity anomalies found beneath the ridges are mapping anisotropic structure into isotropic velocities. After a tomographic inversion incorporating anisotropy models for both ridges, we find that the low‐velocity anomalies found beneath the Nazca Ridge are not anisotropic and therefore likely represent warm mantle, and those beneath the Iquique Ridge are caused by anisotropy. We conclude that subslab mantle buoyancy has a larger impact on the subduction angle than the crustal material of the ridge.
Interdecadal Variations of Radiative Feedbacks Associated With the El Niño and Southern Oscillation (ENSO) in CMIP6 Models
Authors: Tsuchida Ko , Mochizuki Takashi , Kawamura Ryuichi , Kawano Tetsuya 
Published: November 2023

AbstractInternally generated radiative feedback at interannual timescales can influence climate sensitivity estimates. This study has clarified the mechanisms of variability in radiative feedback associated with the El Niño and Southern Oscillation based on preindustrial control simulations for Coupled Model Intercomparison Project Phase 6. Radiative feedback showed large modulation with a comparable magnitude to the intermodel uncertainty associated with internal changes in the zonal gradient of the ocean thermocline and sea surface temperature (SST) in the equatorial Pacific on interdecadal timescales. When the interdecadal SST anomalies in the eastern and western equatorial Pacific were simulated as positive and negative, respectively, the statistical properties of the interannual variations were also modified with diminished Walker circulation changes per unit of global mean surface temperature rise. Meanwhile, the enhanced local Hadley circulation led to a middle‐to upper‐level cloud decrease and a low‐level cloud increase in the subtropics, causing enhanced negative radiative feedback as a global average.
Emergence of Unstable Focused Flow Induced by Variable‐Density Flows in Vertical Fractures
Authors: Cao Hongfan , Yoon Seonkyoo , Xu Zhenyu , Pyrak‐Nolte Laura J. , Bresciani Etienne , Kang Peter K. 
Published: November 2023

AbstractFluids with different densities often coexist in subsurface fractures and lead to variable‐density flows that control subsurface processes such as seawater intrusion, contaminant transport, and geologic carbon sequestration. In nature, fractures have dip angles relative to gravity, and density effects are maximized in vertical fractures. However, most studies on flow and transport through fractures are often limited to horizontal fractures. Here, we study the mixing and transport of variable‐density fluids in vertical fractures by combining three‐dimensional (3D) pore‐scale numerical simulations and visual laboratory experiments. Two miscible fluids with different densities are injected through two inlets at the bottom of a fracture and exit from an outlet at the top of the fracture. Laboratory experiments show the emergence of an unstable focused flow path, which we term a “runlet.” We successfully reproduce the unstable runlet using 3D numerical simulations and elucidate the underlying mechanisms triggering the runlet. Dimensionless number analysis shows that the runlet instability arises due to the Rayleigh‐Taylor instability (RTI), and flow topology analysis is applied to identify 3D vortices that are caused by the RTI. Even under laminar flow regimes, fluid inertia is shown to control the runlet instability by affecting the size and movement of vortices. Finally, we confirm the emergence of a runlet in rough‐walled fractures. Since a runlet dramatically affects fluid distribution, residence time, and mixing, the findings in this study have direct implications for the management of groundwater resources and subsurface applications.
Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels
Authors: Li Guanglei , Chen Yao , Liu Fei , Bi Wenhua , Wang Chenxin , Lu Danfeng , Wen Dan 
Published: November 2023

AbstractIt is important to monitor the intra-/extracellular concentration of hydrogen peroxide (H2O2) in biological processes. However, miniaturized devices that enable portable and accurate H2O2 measurement are still in their infancy because of the difficulty of developing facile sensing strategies and highly integrated sensing devices. In this work, portable H2O2 sensors based on Pt-Ni hydrogels with excellent peroxidase-like and electrocatalytic activities are demonstrated. Thus, simple and sensitive H2O2 sensing is achieved through both colorimetric and electrochemical strategies. The as-fabricated H2O2 sensing chips exhibit favorable performance, with low detection limits (0.030 μM & 0.15 μM), wide linearity ranges (0.10 μM–10.0 mM & 0.50 μM–5.0 mM), outstanding long-term stability (up to 60 days), and excellent selectivity. With the aid of an M5stack development board, portable visual and electrochemical H2O2 sensors are successfully constructed without complicated and expensive equipment or professional operators. When applied to the detection of H2O2 released from HeLa cells, the results obtained by the developed sensors are in good agreement with those from an ultraviolet‒visible spectrophotometer (UV‒vis) (1.97 μM vs. 2.08 μM) and electrochemical station (1.77 μM vs. 1.84 μM).
UBE2J1 is the E2 ubiquitin-conjugating enzyme regulating androgen receptor degradation and antiandrogen resistance
Authors: Rodriguez Tirado Carla , Wang Choushi , Li Xiaoling , Deng Su , Gonzalez Julisa , Johnson Nickolas A. , Xu Yaru , Metang Lauren A. , Sundar Rajan Medha , Yang Yuqiu , Yin Yi , Hofstad Mia , Raj Ganesh V. , Zhang Song , Lemoff Andrew , He Wei , Fan Jie , Wang Yunguan , Wang Tao , Mu Ping 
Published: November 2023

AbstractProstate cancer (PCa) is primarily driven by aberrant Androgen Receptor (AR) signaling. Although there has been substantial advancement in antiandrogen therapies, resistance to these treatments remains a significant obstacle, often marked by continuous or enhanced AR signaling in resistant tumors. While the dysregulation of the ubiquitination-based protein degradation process is instrumental in the accumulation of oncogenic proteins, including AR, the molecular mechanism of ubiquitination-driven AR degradation remains largely undefined. We identified UBE2J1 as the critical E2 ubiquitin-conjugating enzyme responsible for guiding AR ubiquitination and eventual degradation. The absence of UBE2J1, found in 5–15% of PCa patients, results in disrupted AR ubiquitination and degradation. This disruption leads to an accumulation of AR proteins, promoting resistance to antiandrogen treatments. By employing a ubiquitination-based AR degrader to adeptly restore AR ubiquitination, we reestablished AR degradation and inhibited the proliferation of antiandrogen-resistant PCa tumors. These findings underscore the fundamental role of UBE2J1 in AR degradation and illuminate an uncharted mechanism through which PCa maintains heightened AR protein levels, fostering resistance to antiandrogen therapies.