bluuebunny/crossref_metadata_2025_split huggingface dataset:
Giving Breath to Motor Neurons: Noninvasive Mechanical Ventilation Slows Disease Progression in Amyotrophic Lateral Sclerosis
Authors: Grassano Maurizio , Koumantakis Emanuele , Manera Umberto , Canosa Antonio , Vasta Rosario , Palumbo Francesca , Fuda Giuseppe , Salamone Paolina , Marchese Giulia , Casale Federico , Charrier Lorena , Mora Gabriele , Moglia Cristina , Calvo Andrea , Chiò Adriano 
Published: January 2024

ObjectiveNoninvasive mechanical ventilation (NIMV) improves amyotrophic lateral sclerosis (ALS) quality of life and survival. However, data about its effect on disease progression are still lacking. Here, we test whether NIMV use changed the rate of functional decline among ALS patients.MethodsIn this retrospective observational study, we included 448 ALS patients followed up at the ALS Center in Turin, Italy, who underwent NIMV during the disease course. The primary outcome was the change in functional decline after NIMV initiation adjusting for covariates. Functional decline was based on the nonrespiratory items of the Amyotrophic Lateral Sclerosis Functional Rating Scale–Revised (ALSFRS‐R).ResultsNIMV initiation resulted in a slower functional decline (mean improvement = 0.16 points per month, 95% confidence interval = 0.12–0.19, p < 0.001), with consistent effects observed across various demographic factors, including sex, age at diagnosis, and disease duration before NIMV initiation. This finding was replicated using the PRO‐ACT (Pooled Resource Open‐Access ALS Clinical Trials) dataset. The favorable impact of NIMV on ALSFRS‐R progression was evident independently of disease stages. Notably, NIMV benefits were not dose‐dependent but were particularly prominent for nighttime respiratory support.InterpretationNIMV significantly influences the rate of motor progression in ALS, and this effect is not determined by the nonlinearity of ALSFRS‐R trajectory. The functional decline slowed following NIMV initiation, independently of the site of disease onset or disease severity at the time of NIMV initiation. Our findings underscore the importance of timely NIMV initiation for all ALS patients and highlight the need to consider NIMV‐induced slowing of disease progression when evaluating clinical trial outcomes. ANN NEUROL 2024;95:817–822
Application of PC‐SAFT EoS and SCFT for the modeling of thermophysical properties comprising deep eutectic solvent and alcohols
Authors: Mgxadeni Ncomeka , Kabane Bakusele , Hernández Ariel , Bahadur Indra , Mohammad Faruq , Soleiman Ahmed Abdullah , Hashemi Hamed , Kabanda Mwadham M. 
Published: January 2024

AbstractZinc (II) chloride based deep eutectic solvent (DES) were formed by mixing zinc (II) chloride with phosphoric acid. Fourier‐transform infrared spectroscopy was used to identify any possible shifts when the two compounds were assorted, and differential scanning calorimetry (SDT Q600 V20.9 Build 2D) was utilized to evaluate the formation of deep eutectic solvent. Densities,
, and speed of sound, u, of [ZnCl2][phosphoric acid] 1:2.5 with methanol, ethanol, or propanol have been measured at T = (293.15, 303.15, 308.15, and 313.15) K and at atmospheric pressure. Excess molar volumes (
), isentropic compressibilities (
), deviation in isentropic compressibility (
), and intermolecular free length (
) were calculated from the densities, and speed of sound, respectively. To fit the excess molar volumes and deviation in isentropic compressibilities, the Redlich–Kister smoothing polynomial was used. Also, we have used perturbed chain statistical associating fluid theory equation of state (PC‐SAFT EoS) for modeling the densities of the binary mixtures, and Schaaff's collision factor theory (SCFT) and Nomoto's relation (NR) were used for modeling the speed of sounds for the binary mixtures.
A new early‐diverging probainognathian cynodont and a revision of the occurrence of cf. Aleodon from the Chañares Formation, northwestern Argentina: New clues on the faunistic composition of the latest Middle–?earliest Late Triassic Tarjadia Assemblage Zone
Authors: Martinelli Agustín G. , Ezcurra Martín D. , Fiorelli Lucas E. , Escobar Juan , Hechenleitner E. Martín , von Baczko M. Belén , Taborda Jeremías R. A. , Desojo Julia B. 
Published: January 2024

AbstractThe Chañares Formation (Ischigualasto‐Villa Unión Basin) is worldwide known by its exquisitely preserved fossil record of latest Middle‐to‐early Late Triassic tetrapods, including erpetosuchids, “rauisuchians,” proterochampsids, gracilisuchids, dinosauromorphs, pterosauromorphs, kannemeyeriiform dicynodonts, and traversodontid, chiniquodontid and probainognathid cynodonts, coming from the Tarjadia (bottom) and Massetognathus‐Chanaresuchus (top) Assemblage Zones of its lower member. Regarding cynodonts, most of its profuse knowledge comes from the traditional layers discovered by Alfred Romer and his team in the 1960s that are now enclosed in the Massetognathus‐Chanaresuchus Assemblage Zone (AZ). In this contribution we focus our study on the probainognathian cynodonts discovered in levels of the Tarjadia Assemblage Zone. We describe a new chiniquodontid cynodont with transversely broad postcanine teeth (Riojanodon nenoi gen. et sp. nov.) which is related to the genus Aleodon. In addition, the specimen CRILAR‐Pv 567 previously referred to cf. Aleodon is here described, compared, and included in a phylogenetic analysis. It is considered as an indeterminate Aleodontinae nov., a clade here proposed to included chiniquodontids with transversely broad upper and lower postcanines, by having a cuspidated sectorial labial margin and a lingual platform that is twice broader than a lingual cingulum. Cromptodon mamiferoides, from the Cerro de Las Cabras Formation (Cuyo Basin), was also included in the phylogenetic analysis and recovered as an Aleodontinae. The new cynodont and the record of Aleodontinae indet. reinforce the faunal differentiation between the Tarjadia and Massetognathus‐Chanaresuchus Assemblage Zones, in the lower member of the Chañares Formation, and inform on the diverse chiniquodontid clade with both sectorial and transversely broad postcanine teeth.
Distribution of alpha‐synuclein in rat salivary glands
Authors: Nishitani Tomiko , Mitoh Yoshihiro , Yajima Takehiro , Tachiya Daisuke , Hoshika Tomohiro , Fukunaga Tomohiro , Nishitani Yoshihiro , Yoshida Ryusuke , Mizoguchi Itaru , Ichikawa Hiroyuki , Sato Tadasu 
Published: January 2024

AbstractExpression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.
Leader‐following consensus of nonlinear singular switched multi‐agent systems via sliding mode control
Authors: Cai Jiayi , Yi Chengbo , Wu Ying , Liu Danqi , Zhong Deguang 
Published: January 2024

AbstractIn this paper, the sliding mode control algorithm is proposed for solving the leader‐following consensus issue of singular switched multi‐agent systems. The communication topology herein is considered to be directed interacted. To deal with the effects of nonlinearity and singularity, a novel integral‐type sliding mode control protocol is introduced in algorithmic design, which enforces the state trajectories of systems moving on the constructed sliding manifold. The common integral‐type sliding mode gain matrix designed in distributed algorithms herein avoids the frequent switching that may cause the failure and instability of the control framework. Moreover, in the existing works, the developed results on consensus for singular switched multi‐agent systems are almost based on the average dwell time method, and how to construct a desired control input in the frame of the mode‐dependent average dwell time remains a control dilemma. By presenting a general integral‐type sliding mode control rule based on the mode‐dependent average dwell time, this article implements the sliding mode control for the singular switched multi‐agent systems under interest. With the aid of Lyapunov method, the convergence of error systems for multi‐agent systems is obtained. Finally, numerical simulations and application are presented, which illustrate the validity of designed algorithm.
Ligand reaction‐based fluorescent peptide probes for the detection of Cu2+ and glutathione
Authors: Zhou Jiang , Hao Junlei , Wang Dajiang , Wu Jiang , Wang Zhongchang , Lin Pengcheng , Hou Jingcheng 
Published: January 2024

AbstractCopper is a critical element in both human and animal metabolic processes. Its role includes supporting connective tissue cross‐linking, as well as iron and lipid metabolism; at the same time, copper is also a toxic heavy metal that can cause harm to both the environment and human health. Glutathione (GSH) is a tripeptide composed of glutamic acid, cysteine, and glycine combined with sulfhydryl groups. Its properties include acting as an antioxidant and facilitating integrative detoxification. GSH is present in both plant and animal cells and has a fundamental role in maintaining living organisms. GSH is the most abundant thiol antioxidant in the human body. It exists in reduced and oxidized forms within cells and provides significant biochemical functions, such as regulating vitamins such as vitamins D, E, and C, and facilitating detoxification. A fluorescent probe has been developed to detect copper ions selectively, sensitively, and rapidly. This report outlines the successful work on creating a peptide probe, TGN (TPE‐Trp‐Pro‐Gly‐Cln‐His‐NH2), with specific Cu2+ detection capabilities, and a significant fluorescence recovery occurred with the addition of GSH. This indicates that the probe can detect Cu2+ and GSH concurrently. The detection limit for Cu2+ in the buffer solution was 264 nM (R2 = 0.9992), and the detection limit for GSH using the TGN‐Cu2+ complex was 919 nM (R2 = 0.9917). The probe exhibits high cell permeability and low biotoxicity that make it ideal for live cell imaging in biological conditions. This peptide probe has the capability to detect Cu2+ and GSH in biological cells.
Investigating the neuroprotective potential of rAAV2‐PCBP1‐EGFP gene therapy against a 6‐OHDA‐induced model of Parkinson's disease
Authors: Ma Ling‐Yun , Wang Lanying , Liang Jiantao , Huo Lirong 
Published: January 2024

AbstractObjectivesPrevious studies have suggested a potential link between poly(rC)‐binding protein 1 (PCBP1) and neurodegenerative diseases, including Parkinson's disease (PD). However, the precise role of PCBP1 in the pathogenesis of PD remains unclear. Therefore, the main objective of this study was to investigate the neuroprotective effects of PCBP1 in a PD model.MethodsTo evaluate the neuroprotective potential of PCBP1, we conducted cell count assays and observed the expression of heat shock protein 70 (HSP70) in SH‐SY5Y cells exposed to 6‐OHDA‐induced neurotoxicity. Additionally, we utilized recombinant adeno‐associated virus (rAAV2) vectors encoding PCBP1 or EGFP, which were injected into the rat striatum. After 2 weeks of vector or saline injection, 6‐OHDA was administered to the rat striatum. Behavioral assessments using the open field test (OFT) were performed weekly for 7 weeks. At the seventh week after 6‐OHDA injection, immunohistochemistry and protein expression analyses were conducted in the three groups.ResultsThe results indicated that PCBP1 treatment significantly reduced the proliferation of 6‐OHDA‐induced SH‐SY5Y cells. Additionally, in surviving cells, overexpression of PCBP1 enhanced the expression of HSP70. Similarly, rAAV2 vectors effectively delivered PCBP1 into the brain, resulting in sustained expression of rAAV2‐PCBP1‐EGFP. In the OFT, PCBP1 exhibited significant improvements in behavioral abnormalities and reduced anxiety in the PD model rats (p < .01). Moreover, PCBP1 effectively prevented the decrease of tyrosine hydroxylase and HSP70 expression in the lesioned side induced by 6‐OHDA (p < .01). Consistent with expectations, PCBP1 efficiently protected against cell death caused by 6‐OHDA (p < .01).ConclusionsIn conclusion, our findings provide compelling evidence for the beneficial effects of PCBP1 in the PD model, suggesting that PCBP1 could be a potential therapeutic target for PD.
High rate capability and cyclic stability of Ni‐rich layered oxide LiNi0.83Co0.12Mn0.05−xAlxO2 cathodes: Nanofiber versus nanoparticle morphology
Authors: Mitra Soumyadip , Sudakar Chandran 
Published: January 2024

AbstractHigh energy density Ni‐rich layered oxide cathodes LiNi0.83Co0.12Mn0.05−xAlxO2 (x = 0 [NMC], 0.025 [NMCA], 0.05 [NCA]) are fabricated in two different microstructural forms: (i) nanoparticles (NP) and (ii) nanofibers (NF), to evaluate the morphology and compositional effect on the electrochemical properties using same precursors, with the latter fabricated by electrospinning process. Although all the cathodes exhibit a similar crystal structure as confirmed using X‐ray diffraction and Raman spectroscopy, the contrasting difference is observed in their electrochemical properties. XRD and XPS analyses indicate a higher amount of cationic disorder for the NP cathodes compared to their NF counterparts. Nanofibrous Ni‐rich layered oxide cathodes exhibit higher discharge capacities at all C‐rates in comparison to NP cathodes. When cycled at 1C‐rate for 100 cycles, capacity retention of 81% is observed for NCA‐NF, which is superior to all cathodes. Voltage decay as a function of the charge–discharge cycle is found to be low (0.2 mV/cycle) for nanofibrous cathodes compared to 1.5 mV/cycle for NP cathodes. The good rate capability and cyclic stability of nanofibrous Ni‐rich layered oxide cathodes are attributed to a shorter pathway of Li+ diffusion and a large proportion of the active surface area.
Unveiling the Antimicrobial and Larvicidal Potential of Butyrolactones and Orsellinic Acid Derivatives from the Morus alba‐derived Fungus Aspergillus terreus via Integrated In vitro and In silico Approaches
Authors: Amr Khadiga , Elissawy Ahmed M. , Ibrahim Nehal , Elnaggar Mohamed S. , Fawzy Iten M. , Singab Abdel Nasser B. 
Published: January 2024

AbstractThe emergence of multi‐drug‐resistant microbial strains spurred the search for antimicrobial agents; as a result, two distinct approaches were combined: four in vitro studies and four corresponding molecular docking investigations. Antituberculosis, anti‐methicillin‐resistant Staphylococcus aureus (anti‐MRSA), antifungal, and larvicidal activities of the crude extract, two fractions, and seven isolated compounds from Aspergillus terreus derived from Morus alba roots were explored. The isolated compounds (5 butyrolactones and 2 orsellinic acid derivatives) showed potent to moderate antitubercular activity with MIC values ranging from 1.95 to 62.5 μg/mL (compared to isoniazid, 0.24 μg/mL) and promising anti‐MRSA potential with inhibition zone diameters ranging from 8 to 25 mm. Additionally, the in silico study proved that the isolated compounds bind to the two corresponding proteins’ active sites with high to moderate –(C–Docker interaction energies) and stable interactions. The isolated compounds displayed antifungal activities against different fungal strains at diverse degrees of activity, among them compound (8“S,9”)‐dihydroxy‐dihydrobutyrolactone I eliciting the best antifungal activity. Meanwhile, all isolated compounds, fractions, and the crude extract demonstrated extremely selective potent to moderate activity against Cryptococcus neoformans. The isolated five butyrolactone derivatives could develop potential mosquito larvicidal agents as a result of promising docking outcomes in the larval enzyme carboxylesterase.
Intracoronary snaring of the retrograde guidewire: A novel method to solve compartment mismatch in complex retrograde CTO PCI
Authors: Moscardelli Silvia , Condos Gregory , Lombardi William L. , Azzalini Lorenzo 
Published: January 2024

AbstractThe retrograde approach has allowed a remarkable improvement in the success rate of chronic total occlusion (CTO) percutaneous coronary intervention (PCI). After collateral channel crossing, the most crucial aspect of retrograde CTO PCI is creating the connection between the antegrade and retrograde system. Currently, the most common technique to achieve this is reverse controlled antegrade and retrograde subintimal tracking. However, this maneuver sometimes fails due to compartment mismatch (intraplaque situation of one wire and extraplaque situation of the other). New approaches are therefore needed to overcome challenges in this important step of the procedure. Here we present an innovative solution to this problem, which involved capturing the retrograde guidewire (advanced into a side branch at the distal cap) with a microsnare that had been advanced antegradely: this severed the dissection flap separating the antegrade and retrograde system, thus allowing us to successfully recanalize the CTO.
An asymptomatic perforation of the gastrointestinal tract caused by ingestion of foreign body: A case report
Authors: Wang Qiang , Cheng Shujie , Zhang Xuejiao , Xie Tianhao , Sun Xinli , Niu Zheng , Liu Yahan , Jin Xiaoshi 
Published: January 2024

Key Clinical MessageIngestion of foreign bodies is very common in clinical practice. However, gastrointestinal perforation caused by a foreign body is rare, as most foreign bodies can pass the alimentary tract spontaneously or be removed endoscopically. Ingesting a foreign body causes gastrointestinal tract perforation in less than 1% of cases that require surgery. In the past, the literature about gastrointestinal tract perforation caused by foreign bodies had been widely reported worldwide. However, the case of foreign bodies causing gastrointestinal perforation without significant abdominal infection was rarely documented. A 47‐year‐old woman presented with intermittent left lower abdominal pain associated with a mass for 1 month and had no other symptoms. Laparotomy was performed after clinical assessment. During the operation, a local inflammatory mass that adhered to the abdominal wall, part of the small intestine, and sigmoid colon was found in the left lower quarter of the abdominal cavity. The surrounding intestinal wall was edematous. There were two bony foreign bodies in it. Postoperative pathology suggested an inflammatory mass. A foreign body rarely migrates into the abdominal cavity without symptoms that may be related to the omentum's slow perforation process and good function. The best treatment is surgery and using appropriate antibiotics.
5‐Hydroxypyrroloindoline Affords Tryptathionine and 2,2′‐bis‐Indole Peptide Staples: Application to Melanotan‐II
Authors: Todorovic Mihajlo , Blanc Antoine , Wang Zhou , Lozada Jerome , Froelich Juliette , Zeisler Jutta , Zhang Chengcheng , Merkens Helen , Benard Francois , Perrin David M. 
Published: January 2024

AbstractWith peptides increasingly favored as drugs, natural product motifs, namely the tryptathionine staple, found in amatoxins and phallotoxins, and the 2,2’‐bis‐indole found in staurosporine represent unexplored staples for unnatural peptide macrocycles. We disclose the efficient condensation of a 5‐hydroxypyrroloindoline with either a cysteine‐thiol or a tryptophan‐indole to form a tryptathionine or 2‐2’‐bis‐indole staple. Judicious use of protecting groups provides for chemoselective stapling using α‐MSH, which provides a basis for investigating both chemoselectivity and affinity. Both classes of stapled peptides show nanomolar Ki’s, with one showing a sub‐nanomolar Ki value.
Associations of serum trimethylamine N‐oxide and its precursors with colorectal cancer risk in the Prostate, Lung, Colorectal, Ovarian Cancer Screening Trial Cohort
Authors: Byrd Doratha A. , Zouiouich Semi , Karwa Smriti , Li Xinmin S. , Wang Zeneng , Sampson Joshua N. , Loftfield Erikka , Huang Wen‐Yi , Hazen Stanley L. , Sinha Rashmi 
Published: January 2024

AbstractBackgroundDietary intake influences gut microbiome composition, which in turn may be associated with colorectal cancer (CRC). Associations of the gut microbiome with colorectal carcinogenesis may be mediated through bacterially regulated, metabolically active metabolites, including trimethylamine N‐oxide (TMAO) and its precursors, choline, L‐carnitine, and betaine.MethodsProspective associations of circulating TMAO and its precursors with CRC risk were investigated. TMAO, choline, betaine, and L‐carnitine were measured in baseline serum samples from 761 incident CRC cases and 1:1 individually matched controls in the prospective Prostate, Lung, Colorectal, Ovarian Cancer Screening Trial Cohort using targeted fully quantitative liquid chromatography tandem mass spectrometry panels. Prospective associations of the metabolites with CRC risk, using multivariable conditional logistic regression, were measured. Associations of a priori–selected dietary exposures with the four metabolites were also investigated.ResultsTMAO and its precursors were not associated with CRC risk overall, but TMAO and choline were positively associated with higher risk for distal CRC (continuous ORQ90 vs. Q10 [95% CI] = 1.90 [CI, 1.24–2.92; p = .003] and 1.26 [1.17–1.36; p < .0001], respectively). Conversely, choline was inversely associated with rectal cancer (ORQ90 vs. Q10 [95% CI] = 0.77 [0.76–0.79; p < .001]). Red meat, which was previously associated with CRC risk in the Prostate, Lung, Colorectal, Ovarian Cancer Screening Trial Cohort , was positively associated with TMAO (Spearman rho = 0.10; p = .0003).ConclusionsSerum TMAO and choline may be associated with higher risk of distal CRC, and red meat may be positively associated with serum TMAO. These findings provide insight into a potential microbially mediated mechanism underlying CRC etiology.