bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol regulates macrophage recruitment and M1 macrophage polarization and prevents corneal allograft rejection in rats
Authors: Xu Chenjia , Guo Ruilin , Hou Chao , Ma Minglu , Dong Xiaojuan , Ouyang Chen , Wu Jing , Huang Ting 
Published: October 2023

IntroductionResveratrol is an immune modulator that can reduce M1 macrophage polarization in vitro. Reducing macrophage recruitment and M1 polarization can prevent corneal allograft rejection (CGR). In this study, rat corneal allograft rejection models were established to explore the effects of resveratrol on CGR and macrophages and the underlying mechanisms after corneal transplantation.MethodsCorneal allograft models were established, and 100 mg/kg resveratrol was injected intraperitoneally. The corneal allografts were assessed clinically using the Holland rejection scoring system, anterior segment photography, and anterior segment optical coherence tomography. Corneal macrophages, pro-inflammatory cytokines, and corneal lymphatic vessels were detected using hematoxylin and eosin staining, immunofluorescence staining, and real-time quantitative polymerase chain reaction (qRT-PCR). Dendritic cells (DCs) in cervical lymph nodes were explored using flow cytometry. RNA sequencing experiments were conducted to identify the mechanisms through which resveratrol affected CGR. The results were verified using Simple Western analysis. Pro-inflammatory cytokines by macrophages in vitro were measured using qRT-PCR and enzyme-linked immunosorbent assays.ResultsResveratrol significantly prolonged the survival of corneal grafts and reduced graft edema and central corneal thickness. Corneal macrophage recruitment and M1 macrophage polarization decreased significantly after corneal transplantation in the resveratrol group. Resveratrol also reduced pro-inflammatory cytokines in corneal grafts and suppressed the early generation of cornea lymphatic vessels and the recruitment of cornea inflammatory cells 14 days after surgery. Resveratrol decreased the proportion of DCs in ipsilateral cervical lymph nodes. The effect of resveratrol on CGR was related to the phosphatidylinositol 3-kinase/protein kinase-B (PI3K/Akt) pathway. Resveratrol reduced the secretion of pro-inflammatory cytokines by M1 macrophages in vitro.ConclusionOur findings suggest that resveratrol can reduce corneal macrophage recruitment and M1 macrophage polarization after corneal transplantation in rats and prevent CGR. The PI3K/Akt pathway may be an important mechanism that warrants further research.
Species-specificity of the secondary biosynthetic potential in Bacillus
Authors: Yin Qun-Jian , Ying Ti-Ti , Zhou Zhen-Yi , Hu Gang-Ao , Yang Cai-Ling , Hua Yi , Wang Hong , Wei Bin 
Published: October 2023

IntroductionAlthough Bacillus species have produced a wide variety of structurally diverse and biologically active natural products, the secondary biosynthetic potential of Bacillus species is widely underestimated due to the limited number of biosynthetic gene clusters (BGCs) in this genus. The significant variation in the diversity and novelty of BGCs across different species within the Bacillus genus presents a major obstacle to the efficient discovery of novel natural products from Bacillus.MethodsIn this study, the number of each class of BGCs in all 6,378 high-quality Bacillus genomes was predicted using antiSMASH, the species-specificity of BGC distribution in Bacillus was investigated by Principal component analysis. Then the structural diversity and novelty of the predicted secondary metabolites in Bacillus species with specific BGC distributions were analyzed using molecular networking.ResultsOur results revealed a certain degree of species-specificity in the distribution of BGCs in Bacillus, which was mainly contributed by siderophore, type III polyketide synthase (T3PKS), and transAT-PKS BGCs. B. wiedmannii, B. thuringiensis, and B. cereus are rich in RiPP-like and siderophore BGCs, but lack T3PKS BGCs, while B. amyloliquefaciens and B. velezensis are abundant in transAT-PKS BGCs. These Bacillus species collectively encode 77,541 BGCs, with NRPS and RiPPs being the two most dominant types, which are further categorized into 4,291 GCFs. Remarkably, approximately 54.5% of GCFs and 93.8% of the predicted metabolite scaffolds are found exclusively in a single Bacillus species. Notably, B. cereus, B. thuringiensis, and B. velezensis exhibit the highest potential for producing species-specific NRPS and PKS bioinformatic natural products. Taking two species-specific NRPS gene clusters as examples, the potential of Bacillus to synthesize novel species-specific natural products is illustrated.ConclusionThis study highlights the species-specificity of the secondary biosynthetic potential in Bacillus and provides valuable insights for the targeted discovery of novel natural products from this genus.
Changes in lipids and inflammation in adults with super-refractory status epilepticus on a ketogenic diet
Authors: Dickens Alex M. , Johnson Tory P. , Lamichhane Santosh , Kumar Anupama , Pardo Carlos A. , Gutierrez Erie G. , Haughey Norman , Cervenka Mackenzie C. 
Published: October 2023

Introduction: This study aims to test the hypothesis that increased ketone body production resulting from a ketogenic diet (KD) will correlate with reductions in pro-inflammatory cytokines and lipid subspecies and improved clinical outcomes in adults treated with an adjunctive ketogenic diet for super-refractory status epilepticus (SRSE).Methods: Adults (18 years or older) were treated with a 4:1 (fat: carbohydrate and protein) ratio of enteral KD as adjunctive therapy to pharmacologic seizure suppression in SRSE. Blood and urine samples and clinical measurements were collected at baseline (n = 10), after 1 week (n = 8), and after 2 weeks of KD (n = 5). In addition, urine acetoacetate, serum β-hydroxybutyrate, lipidomics, pro-inflammatory cytokines (IL-1β and IL-6), chemokines (CCL3, CCL4, and CXCL13), and clinical measurements were obtained at these three time points. Univariate and multivariate data analyses were performed to determine the correlation between ketone body production and circulating lipids, inflammatory biomarkers, and clinical outcomes.Results: Changes in lipids included an increase in ceramides, mono-hexosylceramide, sphingomyelin, phosphocholine, and phosphoserines, and there was a significant reduction in pro-inflammatory mediators, IL-6 and CXCL13, seen at 1 and 2 weeks of KD. Higher blood β-hydroxybutyrate levels at baseline correlated with better clinical outcomes; however, ketone body production did not correlate with other variables during treatment. Higher chemokine CCL3 levels following treatment correlated with a longer stay in the intensive care unit and a higher modified Rankin Scale score (worse neurologic disability) at discharge and 6-month follow up.Discussion: Adults receiving an adjunctive enteral ketogenic diet for super-refractory status epilepticus exhibit alterations in select pro-inflammatory cytokines and lipid species that may predict their response to treatment.
Establishment and validation of a nomogram to select patients with metastatic sarcomatoid renal cell carcinoma suitable for cytoreductive radical nephrectomy
Authors: Zhou Yulin , Gu Yufeng , Tang Chaopeng , Dong Jie , Xu Song , Sheng Zhengcheng , Zhao Xiaodong , Hu Jun , Shen Tianyi , He Haowei , Yi Xiaoming , Zhou Wenquan , Qu Le , Ge Jingping , Han Conghui 
Published: October 2023

IntroductionMetastatic renal cell carcinoma (mRCC) with sarcomatoid features has a poor prognosis. Cytoreductive radical nephrectomy (CRN) can improve prognosis, but patient selection is unclear. This study aimed to develop a prediction model for selecting patients suitable for CRN.Materials and methodsPatients with a diagnosis of mRCC with sarcomatoid features in the Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2015 were retrospectively reviewed. CRN benefit was defined as a survival time longer than the median overall survival (OS) in patients who did not receive CRN. A prediction nomogram was established and validated using the SEER cohort (training and internal validation) and an external validation cohort.ResultsOf 900 patients with sarcomatoid mRCC, 608 (67.6%) underwent CRN. OS was longer in the CRN group than in the non-CRN group (8 vs. 6 months, hazard ratio (HR) = 0.767, p = 0.0085). In the matched CRN group, 124 (57.7%) patients survived &gt;6 months after the surgery and were considered to benefit from CRN. Age, T-stage, systematic therapy, metastatic site, and lymph nodes were identified as independent factors influencing OS after CRN, which were included in the prediction nomogram. The monogram performed well on the training set (area under the receiver operating characteristic (AUC) curve = 0.766, 95% confidence interval (CI): 0.687–0.845), internal validation set (AUC = 0.796, 95% CI: 0.684–0.908), and external validation set (AUC = 0.911, 95% CI: 0.831–0.991).ConclusionsA nomogram was constructed and validated with good accuracy for selecting patients with sarcomatoid mRCC suitable for CRN.
Bibliometric analysis and visualization of research trends on HIV-1 capsid inhibitors (2000–2022)
Authors: Sun Lin , Zhang Tongchao , Xu Shujing , Zhang Xujie , Zhan Peng , Liu Xinyong 
Published: October 2023

Background: Acquired immunodeficiency syndrome (AIDS) has seriously endangered human life and health, the main pathogenic agent is human immunodeficiency virus type 1 (HIV-1). The combination antiretroviral therapy (cART) has shown serious drug resistance and side effects, and the discovery of HIV-1 capsid inhibitors is an effective way to solve the problem. Recent studies have shown significant progress in the research of HIV-1 capsid inhibitors. However, there is still a lack of comprehensive overview of bibliometric analysis in this field. This study aimed to provide the research trends and hotspots of HIV-1 capsid inhibitors.Method: Publications related to HIV-1 capsid inhibitors from 2000 to 2022 were searched on the Web of Science Core Collection (WoSCC) database and screened according to inclusion criteria. VOSviewer was conducted to evaluate the results.Results: 96 publications from 25 countries were finally included, and the number of annual publications related to HIV-1 capsid inhibitors showed an increasing trend. The United States was the most productive country with the most publication number, H-index, and total citation number, as well as the widest international cooperation. The most popular journal in this field was Journal of Virology. Drexel University was the most productive institution, and Simon Cocklin participated in the most publications. Keywords co-occurrence analysis exhibited that studying the molecular mechanism of capsid protein, discovering drug candidates, and improving antiretroviral therapy are the main and hot topics in this field.Conclusion: This is the first bibliometric study in the field of HIV-1 capsid inhibitors, which comprehensively analyzed the research trends and hotspots in this direction. This work is expected to provide the scientific community with new insights to promote the research of HIV-1 capsid inhibitors.
Genomic analyses of Asian honeybee from the Sansha Island in the South China Sea, suggest it’s evolutional origin and environmental adaption
Authors: Gao Jinglin , Tang Xiangyou , Zhao Shan , Tao Kunlin , Shi Xinyan , Song Huali , Yao Yuxin , Jiang Yan , Wang Tianbin , Li Xiang , Zhao Dongxiang , Xu Jinshan 
Published: October 2023

Discovering new resources and enhancing our knowledge of distribution are crucial for the preservation and utilization of honeybee genetic resources. Our research focused on morphological and population genetic analysis, which revealed significant differences in the SanshaId group compared to others. Notably, the SanshaId group displayed smaller tongue length, averaging at 3.83 mm, and a larger fixation index, indicating a distinct level of subspecies differentiation (Fst = 0.2669). We therefore assumed that A.cerana sanshasis is a new ecotype and subspecies of A. cerana. Estimates of population history indicated that SanshaId population is most closely related to HainanId population, which differed from SanshaId population by about 0.57 Ma due to geological movements. We identified 131 high-frequency non-synonymous mutant genes in the SanshaId group compared to HainanId group. Among these genes, Cuticular genes related to tongue morphology were subject to evolutionary selection, and some genes related to glucose metabolism were highly expressed in the gut. Our results expand the understanding of the distribution range of Asian honeybee and provide a basis for understanding the population dynamics and evolutionary adaptation of A. cerana sanshasis in tropical island environments.
An Integrated Molecular Networking and Docking Approach to Characterize the Metabolome of Helichrysum splendidum and Its Pharmaceutical Potentials
Authors: Lephatsi Motseoa Mariam , Choene Mpho Susan , Kappo Abidemi Paul , Madala Ntakadzeni Edwin , Tugizimana Fidele 
Published: October 2023

South Africa is rich in diverse medicinal plants, and it is reported to have over 35% of the global Helichrysum species, many of which are utilized in traditional medicine. Various phytochemical studies have offered valuable insights into the chemistry of Helichrysum plants, hinting at bioactive components that define the medicinal properties of the plant. However, there are still knowledge gaps regarding the size and diversity of the Helichrysum chemical space. As such, continuous efforts are needed to comprehensively characterize the phytochemistry of Helichrysum, which will subsequently contribute to the discovery and exploration of Helichrysum-derived natural products for drug discovery. Thus, reported herein is a computational metabolomics work to comprehensively characterize the metabolic landscape of the medicinal herb Helichrysum splendidum, which is less studied. Metabolites were methanol-extracted and analyzed on a liquid chromatography–tandem mass spectrometry (LC-MS/MS) system. Spectral data were mined using molecular networking (MN) strategies. The results revealed that the metabolic map of H. splendidum is chemically diverse, with chemical superclasses that include organic polymers, benzenoids, lipid and lipid-like molecules, alkaloids, and derivatives, phenylpropanoids and polyketides. These results point to a vastly rich chemistry with potential bioactivities, and the latter was demonstrated through computationally assessing the binding of selected metabolites with CDK-2 and CCNB1 anti-cancer targets. Molecular docking results showed that flavonoids (luteolin, dihydroquercetin, and isorhamnetin) and terpenoids (tiliroside and silybin) interact strongly with the CDK-2 and CCNB1 targets. Thus, this work suggests that these flavonoid and terpenoid compounds from H. splendidum are potentially anti-cancer agents through their ability to interact with these proteins involved in cancer pathways and progression. As such, these actionable insights are a necessary step for further exploration and translational studies for H. splendidum-derived compounds for drug discovery.
THE ORDER OF CHILDBIRTH IN THE FAMILY AND ITS INFLUENCE ON PERSONALITY DEVELOPMENT (USING THE PSYCHOANALYTIC PEDAGOGY OF ALFRED ADLER AS AN EXAMPLE)
Authors: Vertel A. 
Published: October 2023

The article reveals the relationship between the birth order of a child in a family and their personal characteristics. The state of development of the investigated topic in pedagogical science is analyzed. It has been proved that this is a rather significant and relevant scientific problem of the research field of modern pedagogy. S. Freud also claimed that the position of a child among siblings is of great importance in later life, and older and younger children have a number of identical characterological traits. A. Adler indicated the connection between the order of a child’s birth and the formation of his personality, highlighting the ordinal positions of the child in the family. A. Adler discovered that people who occupy similar positions in the family in order of birth have very similar psychological characteristics. He argued that the order in which a child is born in a family is quite an influential factor for a person’s entire destiny, including his career and social relations.
The dependence and interrelationship of birth order with such factors as biological differences, intelligence, peculiarities of personality development, mental illnesses are substantiated. The general characteristics of the positions of the only, first, middle and youngest child in the family are explained. Peculiarities of the family situation of development, typical problems, and personal characteristics of each of the specified positions are considered.
Our research is dictated by the need to develop such an approach to personality development, which considers it not only as formed under the influence of exclusively genetic factors, but also takes into account the social prerequisites of his development. Emphasis is placed on the fact that the influence of birth order should be studied through the prism of other factors, which involve taking into account the historical and cultural context of upbringing, as well as considering the family as a system.
Reduction of working temperature for the large magnitude in thermoelectric dimensionless figure of merit of Ag2−x
Cu
x
S
Authors: Sato Kosuke , Kim Gareoung , Murase Koki , Singh Saurabh , Hirata Keisuke , Byeon Dogyun , Matsunami Masaharu , Takeuchi Tsunehiro 
Published: October 2023

Abstract
In this study, we try to reduce the temperature range for the large magnitude of dimensionless figure of merit ZT = 20 that was observed for the Ag2S composite consisting of low- and high-temperature phases under a unique temperature gradient at around 400 K. It reveals that partial substitution of Cu for Ag sites in Ag2S reduces the phase transition temperature, and subsequently the temperature range for this high ZT down to a temperature of 373 K. This result strongly suggests that our developed Cu-substituted Ag2S could be one of the best thermoelectric component materials in the generators capable of effectively recovering electric power from heat exchangers using hot water as a working liquid.
АДМІНІСТРАТИВНО-ПРАВОВИЙ СТАТУС ПРАВООХОРОННИХ ОРГАНІВ: ПОНЯТТЯ І СТРУКТУРА
Authors: Tokarieva Kseniia 
Published: October 2023

Стаття присвячена дослідженню сучасних поглядів на поняття і структуру адміністративно-правового статусу правоохоронних органів. Адміністративно-правове забезпечення правоохоронних органів, зокрема регламентація адміністративно-правового статусу, повинні всіляко сприяти досягненню загальної мети їх створення – забезпечення прав людини, демократії та розбудови правової держави. Встановлено, що адміністративно-правовий статус правоохоронних органів в Україні – це категорія, яка включає в себе нормативно визначені порядок створення та ліквідації, права та обов’язки, повноваження, визначені межі компетенції, принципи, мету, функції, організаційну структуру, гарантії та відповідальність правоохоронних органів, а також відображає місце таких органів у адміністративно-правових відносинах з громадянами, між собою чи з іншими суб’єктами права
Mucinous Cystadenoma of the Cystic Duct
Authors: Olimov B. P. , Balabin N. S. , Kuleznova J. V. , Vorobyov R. V. , Lapteva M. G. , Vankovich A. N. , Tsvirkun V. V. 
Published: October 2023

A rare clinical observation of a cystic formation of the biliary system — mucinous cystadenoma of the cystic duct is presented. This type of tumors is benign, but in 20 % of cases their malignancy is described. They are often detected incidentally during routine examination and require surgical treatment, regardless of clinical manifestations. Difficulties in differential diagnosis led to unreasonably long follow-up, ending with malignancy of cystadenomas, which reduces the possibility of radical surgical treatment. Therefore, timely detection and differentiation of biliary mucinous cystadenomas by examination are very important for treatment strategy.
Bimbingan Teknis Perancangan Jaringan dan Keamanan Internet bagi Siswa SMK
Authors: Hidayat Syamsu , Silvanie Astried , Rifiyanti Hafizah , Syah Silvana , Kurniawan Ari 
Published: October 2023

Di era digital saat ini keterampilan dalam rancangan jaringan dan keamanan internet sangat dibutuhkan di berbagai industri. Bagi para siswa SMK perlu dibekali dengan pengetahuan dan keterampilan yang diperlukan untuk merakit dan memelihara jaringan serta memastikan keamanannya. Panduan ini membantu mereka mengembangkan kompetensi yang relevan dengan industri dan meningkatkan kemampuan kerja mereka. Pelaksanaan kegiatan pengabdian masyarakat dilakukan di SMK 3 Perguruan Cikini Jakarta Selatan. Metode pelaksanaan kegiatan pengabdian masyarakat berupa presentasi dan bimbingan teknis praktek langsung di laboratorium komputer tentang perancangan jaringan dan keamanan internet. Para siswa belajar tentang peran firewall dalam menyaring lalu lintas jaringan dan melindungi dari akses yang tidak sah, serta praktek langsung proyek desain untuk membuat arsitektur jaringan berdasarkan persyaratan dan batasan tertentu. Kegiatan ini memberikan banyak kesempatan kepada para siswa untuk menerapkan pengetahuan mereka dalam skenario dunia nyata. Melalui proyek desain jaringan dan latihan konfigurasi keamanan, para siswa dapat menerjemahkan konsep teoritis ke dalam solusi praktis. Pendekatan langsung ini berkontribusi pada pemahaman dan pengembangan keterampilan mereka secara keseluruhann.
Entorno Virtual de Capacitación para la Formación de Competencias Digitales en Docentes de Básica Superior de Guayaquil – Ecuador
Authors: Mendoza Castañeda Gilberto Antonio , Chávez Arizala Noé David , Valencia Bucay Vanessa Stefania , García Muñiz Jacqueline Alexandra , Carrión Peña Mónica Cecilia 
Published: October 2023

La investigación tuvo como objetivo general, elaborar un entorno virtual de aprendizaje para la capacitación de los docentes de Básica Superior de la Unidad Educativa Fiscal Martha Bucaram de Roldós, que estimule el uso de actividades interactivas, autodirigidas y de producción de contenidos, basados en la contextualización de las áreas de competencias definidas por el Marco Común Europeo de Competencias digitales docentes; dirigida a resolver el problema de investigación referido a ¿Cómo mejorar las competencias digitales en los docentes de la básica superior de la Unidad Educativa Fiscal Martha Bucaram de Roldós?; aportándose un entorno virtual de aprendizaje para la capacitación docente en competencias digitales, para estimular el uso de actividades interactivas, autodirigidas y de producción de contenidos, diseñado particularmente para docentes de básica superior de la Unidad Educativa Fiscal Martha Bucaram de Roldós. Se desarrolló una investigación de tipo aplicada, con un enfoque mixto, donde se aplicaron métodos cuantitativos y cualitativos. Los métodos aplicados fueron teóricos (Análisis y síntesis, Inductivo – deductivo y de Modelación), empíricos (Observación, Análisis documental, técnicas como la Encuesta y el cuestionario) y estadísticos matemáticos para la aplicación de pruebas que permitieron el procesamiento de la información generada y la validación de instrumentos.