bluuebunny/crossref_metadata_2025_split huggingface dataset:
GROUP RIGHTS, GENDER JUSTICE, AND WOMEN’S SELF-HELP GROUPS: EXIT, VOICE, AND LOYALTY IN AN INDIGENOUS COMMUNITY IN INDIA
Authors: Kabeer Naila , Narain Nivedita , Arora Varnica , Lal Vinitika 
Published: February 2024

AbstractThis essay addresses tensions within political philosophy between group rights, which allow historically marginalized communities some self-governance in determining its own rules and norms, and the rights of marginalized subgroups, such as women, within these communities. Community norms frequently uphold patriarchal structures that define women as inferior to men, assign them a subordinate status within the community, and cut them off from the individual rights enjoyed by women in other sections of society. As feminists point out, the capacity for voice and exit cannot be taken for granted, for community norms may be organized in ways that deny women any voice in its decision-making forums as well as the resources they would need to survive outside the community. This essay draws on research among the Gond, an indigenous community in India, to explore this debate. Given the strength of the forces within the community militating against women’s capacity for voice or exit, the question motivating our research is: Can external organizations make a difference? We explore the impacts of two external development organizations that sought to work with women within these communities in order to answer this question.
Population size is not a reliable indicator of seed germination
Authors: Baskin Jerry M. , Baskin Carol C. 
Published: February 2024

AbstractSmall isolated plant populations are one of the consequences of fragmentation of natural habitats by humans. We asked what effect does the creation of smaller populations from larger ones has on the plant fitness-related trait seed germination. Using information on 119 species (142 species entries) in 50 families, we found that seeds in only 35.2% of the species entries from larger populations germinated to higher percentages than those from smaller populations. In the other entries, seeds from large and small populations germinated equally well (57.7% of total entries) or seeds from small populations germinated better (7.0% of total entries) than those from large populations. These results indicate that population size is not a reliable predictor of seed germinability. Furthermore, there was little relationship between seed germination and either seed mass, genetic diversity or degree of population isolation, or between population size and genetic diversity.
Increasing food insecurity severity is associated with lower diet quality
Authors: Kent Katherine , Schumacher Tracy , Kocar Sebastian , Seivwright Ami , Visentin Denis , Collins Clare E , Lester Libby 
Published: February 2024

Abstract

Objective:
Food insecurity may reduce diet quality, but the relationship between food insecurity severity and diet quality is under-researched. This study aimed to examine the relationship between diet quality and severity of household food insecurity.

Design:
A cross-sectional, online survey used the United States Department of Agriculture Household Food Security Six-item Short Form to classify respondents as food secure or marginally, moderately or severely food insecure. The Australian Recommended Food Score (ARFS; scored 0–73) determined diet quality (ARFS total and sub-scale scores). Survey-weighted linear regression (adjusted for age, sex, income, education, location and household composition) was conducted.

Setting:
Tasmania, Australia.

Participants:
Community-dwelling adults (aged 18 years and over).

Results:
The mean ARFS total for the sample (n 804, 53 % female, 29 % aged > 65 years) was 32·4 (sd = 9·8). As the severity of household food insecurity increased, ARFS total decreased. Marginally food-insecure respondents reported a mean ARFS score three points lower than food-secure adults (B = –2·7; 95 % CI (–5·11, –0·34); P = 0·03) and reduced by six points for moderately (B = –5·6; 95 % CI (–7·26, –3·90); P < 0·001) and twelve points for severely food-insecure respondents (B = –11·5; 95 % CI (–13·21, –9·78); P < 0·001). Marginally food-insecure respondents had significantly lower vegetable sub-scale scores, moderately food-insecure respondents had significantly lower sub-scale scores for all food groups except dairy and severely food-insecure respondents had significantly lower scores for all sub-scale scores.

Conclusions:
Poorer diet quality is evident in marginally, moderately and severely food-insecure adults. Interventions to reduce food insecurity and increase diet quality are required to prevent poorer nutrition-related health outcomes in food-insecure populations in Australia.
DDX3X interacts with SIRT7 to promote PD-L1 expression to facilitate PDAC progression
Authors: Zhao Tianming , Zhu Hanlong , Zou Tianhui , Zhao Si , Zhou Lin , Ni Muhan , Liu Feng , Zhu Hao , Dou Xiaotan , Di Jian , Xu Bing , Wang Lei , Zou Xiaoping 
Published: February 2024

Abstract
Pancreatic ductal adenocarcinoma (PDAC) is recognized as the most aggressive and fatal malignancy. A previous study reported that PDAC patients who exhibit elevated levels of DDX3X have a poor prognosis and low overall survival rate. However, the underlying molecular mechanism remains unclear. This study aimed to investigate the specific roles of DDX3X in PDAC. Multiple bioinformatics analyses were used to evaluate DDX3X expression and its potential role in PDAC. In vitro and in vivo studies were performed to assess the effects of DDX3X on PDAC cell growth. Furthermore, Western blotting, quantitative PCR, immunohistochemistry, immunofluorescence, mass spectrometry, coimmunoprecipitation and multiplexed immunohistochemical staining were conducted to identify the specific regulatory mechanism in PDAC. The results verified that DDX3X expression is notably upregulated in the tumor tissue vs. normal tissue of PDAC patients. DDX3X knockdown markedly suppressed the proliferation, invasion and migration of PDAC cells in vitro and inhibited tumor growth in vivo. Conversely, overexpression of DDX3X induced the opposite effect. Further studies supported that the DDX3X protein can associate with sirtuin 7 (SIRT7) to stimulate PDAC carcinogenesis and progression. Furthermore, SIRT7 inhibition significantly impeded DDX3X-mediated tumor growth both ex vivo and in vivo. The results also revealed that programmed death ligand 1 (PD-L1) expression is positively correlated with DDX3X expression. These results reveal significant involvement of the DDX3X-SIRT7 axis in the initiation and advancement of PDAC and offer previously undiscovered therapeutic options for PDAC management.
Targeting the prefrontal-supplementary motor network in obsessive-compulsive disorder with intensified electrical stimulation in two dosages: a randomized, controlled trial
Authors: Alizadehgoradel Jaber , Molaei Behnam , Barzegar Jalali Khandan , Pouresmali Asghar , Sharifi Kiomars , Hallajian Amir-Homayun , Nejati Vahid , Glinski Benedikt , Vicario Carmelo M. , Nitsche Michael A. , Salehinejad Mohammad Ali 
Published: February 2024

AbstractObsessive-compulsive disorder (OCD) is associated with a high disease burden, and treatment options are limited. We used intensified electrical stimulation in two dosages to target a main circuitry associated with the pathophysiology of OCD, left dorsolateral prefrontal cortex (l-DLPFC), and pre-supplementary motor area (pre-SMA) and assessed clinical outcomes, neuropsychological performance, and brain physiology. In a double-blind, randomized controlled trial, thirty-nine patients with OCD were randomly assigned to three groups of sham, 2-mA, or 1-mA transcranial direct current stimulation (tDCS) targeting the l-DLPFC (F3) and pre-SMA (FC2) with anodal and cathodal stimulation respectively. The treatment included 10 sessions of 20-minute stimulation delivered twice per day with 20-min between-session intervals. Outcome measures were reduction in OCD symptoms, anxiety, and depressive states, performance on a neuropsychological test battery (response inhibition, working memory, attention), oscillatory brain activities, and functional connectivity. All outcome measures except EEG were examined at pre-intervention, post-intervention, and 1-month follow-up times. The 2-mA protocol significantly reduced OCD symptoms, anxiety, and depression states and improved quality of life after the intervention up to 1-month follow-up compared to the sham group, while the 1-mA protocol reduced OCD symptoms only in the follow-up and depressive state immediately after and 1-month following the intervention. Both protocols partially improved response inhibition, and the 2-mA protocol reduced attention bias to OCD-related stimuli and improved reaction time in working memory performance. Both protocols increased alpha oscillatory power, and the 2-mA protocol decreased delta power as well. Both protocols increased connectivity in higher frequency bands at frontal-central areas compared to the sham. Modulation of the prefrontal-supplementary motor network with intensified tDCS ameliorates OCD clinical symptoms and results in beneficial cognitive effects. The 2-mA intensified stimulation resulted in larger symptom reduction and improved more converging outcome variables related to therapeutic efficacy. These results support applying the intensified prefrontal-SMA tDCS in larger trials.
IL-17 promotes osteoclast-induced bone loss by regulating glutamine-dependent energy metabolism
Authors: Peng Renpeng , Dong Yimin , Zheng Meng , Kang Honglei , Wang Pengju , Zhu Meipeng , Song Kehan , Wu Wei , Li Feng 
Published: February 2024

AbstractOsteoclasts consume an amount of adenosine triphosphate (ATP) to perform their bone resorption function in the development of osteoporosis. However, the mechanism underlying osteoclast energy metabolism has not been fully elucidated. In addition to glucose, glutamine (Glu) is another major energy carrier to produce ATP. However, the role of Glu metabolism in osteoclasts and the related molecular mechanisms has been poorly elucidated. Here we show that Glu is required for osteoclast differentiation and function, and that Glu deprivation or pharmacological inhibition of Glu transporter ASCT2 by V9302 suppresses osteoclast differentiation and their bone resorptive function. In vivo treatment with V9302 improved OVX-induced bone loss. Mechanistically, RNA-seq combined with in vitro and in vivo experiments suggested that Glu mediates the role of IL-17 in promoting osteoclast differentiation and in regulating energy metabolism. In vivo IL-17 treatment exacerbated OVX-induced bone loss, and this effect requires the participation of Glu or its downstream metabolite α-KG. Taken together, this study revealed a previously unappreciated regulation of IL-17 on energy metabolism, and this regulation is Glu-dependent. Targeting the IL-17–Glu–energy metabolism axis may be a potential therapeutic strategy for the treatment of osteoporosis and other IL-17 related diseases.
The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome
Authors: Krischuns Tim , Arragain Benoît , Isel Catherine , Paisant Sylvain , Budt Matthias , Wolff Thorsten , Cusack Stephen , Naffakh Nadia 
Published: February 2024

AbstractThe current model is that the influenza virus polymerase (FluPol) binds either to host RNA polymerase II (RNAP II) or to the acidic nuclear phosphoprotein 32 (ANP32), which drives its conformation and activity towards transcription or replication of the viral genome, respectively. Here, we provide evidence that the FluPol-RNAP II binding interface, beyond its well-acknowledged function in cap-snatching during transcription initiation, has also a pivotal role in replication of the viral genome. Using a combination of cell-based and in vitro approaches, we show that the RNAP II C-terminal-domain, jointly with ANP32, enhances FluPol replication activity. We observe successive conformational changes to switch from a transcriptase to a replicase conformation in the presence of the bound RNPAII C-terminal domain and propose a model in which the host RNAP II is the anchor for transcription and replication of the viral genome. Our data open new perspectives on the spatial coupling of viral transcription and replication and the coordinated balance between these two activities.
Designing lithium halide solid electrolytes
Authors: Wang Qidi , Zhou Yunan , Wang Xuelong , Guo Hao , Gong Shuiping , Yao Zhenpeng , Wu Fangting , Wang Jianlin , Ganapathy Swapna , Bai Xuedong , Li Baohua , Zhao Chenglong , Janek Jürgen , Wagemaker Marnix 
Published: February 2024

AbstractAll-solid-state lithium batteries have attracted widespread attention for next-generation energy storage, potentially providing enhanced safety and cycling stability. The performance of such batteries relies on solid electrolyte materials; hence many structures/phases are being investigated with increasing compositional complexity. Among the various solid electrolytes, lithium halides show promising ionic conductivity and cathode compatibility, however, there are no effective guidelines when moving toward complex compositions that go beyond ab-initio modeling. Here, we show that ionic potential, the ratio of charge number and ion radius, can effectively capture the key interactions within halide materials, making it possible to guide the design of the representative crystal structures. This is demonstrated by the preparation of a family of complex layered halides that combine an enhanced conductivity with a favorable isometric morphology, induced by the high configurational entropy. This work provides insights into the characteristics of complex halide phases and presents a methodology for designing solid materials.
Synergistic denitrification mechanism of domesticated aerobic denitrifying bacteria in low-temperature municipal wastewater treatment
Authors: Wang Fan , Cui Qin , Liu Wenai , Jiang Weiqing , Ai Shengshu , Liu Wanqi , Bian Dejun 
Published: February 2024

AbstractTo address the problems of low efficacy and low microbial activity in low-temperature municipal wastewater treatment, this study utilized an air-lift micro-pressure internal circulation integrated reactor (AMICIR). Through controlling the amount of aeration and dissolved oxygen (DO) in the reactor, AMICIR creates alternating aerobic and anaerobic environments, explores the enrichment conditions of aerobic denitrifying bacteria, examines the changes in pollutant removal efficiency and the characteristics of bacterial colony structure during the process of enrichment of aerobic denitrifying bacteria in the system, and reveals the mechanism of nitrogen removal by aerobic denitrifying bacteria cooperating with anaerobic denitrifying bacteria in the low-temperature municipal wastewater treatment system. Experimental results showed average removal rates of NH4+-N, chemical oxygen demand (COD), total phosphorus (TP), and total nitrogen (TN) reaching 93.85%, 89.30%, 92.75%, and 75.4%, respectively. The microorganisms secreted large amounts of proteins and polysaccharides, forming zoogloea and anaerobic microenvironments conducive to traditional denitrification reactions. IlluminaMiSeq sequencing analysis revealed the presence of anaerobic phyla. The system was enriched with a large number of microorganisms, and aerobic denitrifying bacteria (Flavobacterium, Rhodoferax, and Pseudomonas) were successfully cultured. Flavobacterium emerged as the dominant species, with relative abundance ranging from 18.56% to 22.60%. Functional gene prediction indicated high abundance of aerobic denitrification genes, such as napA. Aerobic denitrifying bacteria were successfully enriched in the system to improve nitrogen removal from municipal wastewater at low temperatures.
Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores
Authors: Datler Julia , Hansen Jesse M. , Thader Andreas , Schlögl Alois , Bauer Lukas W. , Hodirnau Victor-Valentin , Schur Florian K. M. 
Published: February 2024

AbstractPoxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses.
Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair
Authors: Kokic Goran , Yakoub George , van den Heuvel Diana , Wondergem Annelotte P. , van der Meer Paula J. , van der Weegen Yana , Chernev Aleksandar , Fianu Isaac , Fokkens Thornton J. , Lorenz Sonja , Urlaub Henning , Cramer Patrick , Luijsterburg Martijn S. 
Published: February 2024

AbstractDuring transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that allows for removal of DNA lesions. This transition requires site-specific ubiquitylation of Pol II by the CRL4CSA ubiquitin ligase, a process that is facilitated by ELOF1 in an unknown way. Using cryogenic electron microscopy, biochemical assays and cell biology approaches, we found that ELOF1 serves as an adaptor to stably position UVSSA and CRL4CSA on arrested Pol II, leading to ligase neddylation and activation of Pol II ubiquitylation. In the presence of ELOF1, a transcription factor IIS (TFIIS)-like element in UVSSA gets ordered and extends through the Pol II pore, thus preventing reactivation of Pol II by TFIIS. Our results provide the structural basis for Pol II ubiquitylation and inactivation in TCR.
A general class of improved population variance estimators under non-sampling errors using calibrated weights in stratified sampling
Authors: Pandey M. K. , Singh G. N. , Zaman Tolga , Mutairi Aned Al , Mustafa Manahil SidAhmed 
Published: February 2024

AbstractThis paper proposes a new calibration estimator for population variance within a stratified two-phase sampling design. It takes into account random non-response and measurement errors, specifically applying this method to estimate the variance in Gas turbine exhaust pressure data. The study integrates additional information from two highly positively correlated auxiliary variables to develop a general class of estimators tailored for the stratified two-phase sampling scheme. The properties of these estimators, in terms of their biases and mean square errors, have been thoroughly examined and extensively analyzed through numerical and simulation studies. Furthermore, the calibrated weights of the strata are derived. The proposed estimators outperform the natural estimator of population variance. Finally, suitable recommendations have been made for survey statisticians intending to apply these findings to real-life problems.
Deep brain stimulation of the central thalamus restores arousal and motivation in a zolpidem-responsive patient with akinetic mutism after severe brain injury
Authors: Arnts Hisse , Tewarie Prejaas , van Erp Willemijn , Schuurman Rick , Boon Lennard I. , Pennartz Cyriel M. A. , Stam Cornelis J. , Hillebrand Arjan , van den Munckhof Pepijn 
Published: February 2024

AbstractAfter severe brain injury, zolpidem is known to cause spectacular, often short-lived, restorations of brain functions in a small subgroup of patients. Previously, we showed that these zolpidem-induced neurological recoveries can be paralleled by significant changes in functional connectivity throughout the brain. Deep brain stimulation (DBS) is a neurosurgical intervention known to modulate functional connectivity in a wide variety of neurological disorders. In this study, we used DBS to restore arousal and motivation in a zolpidem-responsive patient with severe brain injury and a concomitant disorder of diminished motivation, more than 10 years after surviving hypoxic ischemia. We found that DBS of the central thalamus, targeted at the centromedian-parafascicular complex, immediately restored arousal and was able to transition the patient from a state of deep sleep to full wakefulness. Moreover, DBS was associated with temporary restoration of communication and ability to walk and eat in an otherwise wheelchair-bound and mute patient. With the use of magnetoencephalography (MEG), we revealed that DBS was generally associated with a marked decrease in aberrantly high levels of functional connectivity throughout the brain, mimicking the effects of zolpidem. These results imply that ‘pathological hyperconnectivity’ after severe brain injury can be associated with reduced arousal and behavioral performance and that DBS is able to modulate connectivity towards a ‘healthier baseline’ with lower synchronization, and, can restore functional brain networks long after severe brain injury. The presence of hyperconnectivity after brain injury may be a possible future marker for a patient’s responsiveness for restorative interventions, such as DBS, and suggests that lower degrees of overall brain synchronization may be conducive to cognition and behavioral responsiveness.
Minimizing artifact-induced false-alarms for seizure detection in wearable EEG devices with gradient-boosted tree classifiers
Authors: Ingolfsson Thorir Mar , Benatti Simone , Wang Xiaying , Bernini Adriano , Ducouret Pauline , Ryvlin Philippe , Beniczky Sandor , Benini Luca , Cossettini Andrea 
Published: February 2024

AbstractElectroencephalography (EEG) is widely used to monitor epileptic seizures, and standard clinical practice consists of monitoring patients in dedicated epilepsy monitoring units via video surveillance and cumbersome EEG caps. Such a setting is not compatible with long-term tracking under typical living conditions, thereby motivating the development of unobtrusive wearable solutions. However, wearable EEG devices present the challenges of fewer channels, restricted computational capabilities, and lower signal-to-noise ratio. Moreover, artifacts presenting morphological similarities to seizures act as major noise sources and can be misinterpreted as seizures. This paper presents a combined seizure and artifacts detection framework targeting wearable EEG devices based on Gradient Boosted Trees. The seizure detector achieves nearly zero false alarms with average sensitivity values of $$65.27\%$$

65.27
%

for 182 seizures from the CHB-MIT dataset and $$57.26\%$$

57.26
%

for 25 seizures from the private dataset with no preliminary artifact detection or removal. The artifact detector achieves a state-of-the-art accuracy of $$93.95\%$$

93.95
%

(on the TUH-EEG Artifact Corpus dataset). Integrating artifact and seizure detection significantly reduces false alarms—up to $$96\%$$

96
%

compared to standalone seizure detection. Optimized for a Parallel Ultra-Low Power platform, these algorithms enable extended monitoring with a battery lifespan reaching 300 h. These findings highlight the benefits of integrating artifact detection in wearable epilepsy monitoring devices to limit the number of false positives.