bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anticoagulant protein C p.Lys193del mutation and promoter region polymorphisms :common genetic risk factors of deep venous thrombosis in Chinese population
Authors: Wei Xuqian , Yi Xiaolei , Chen Weibin , Tang Jin , Dai Jing 
Published: January 2024

Abstract
Background:
Deep venous thrombosis (DVT) is a common condition that is fatal and has a high occurrence. The aim of this study was to examine the prevalence of anticoagulant protein C (PC) mutation p.Lys193del and promoter polymorphisms in patients with DVT in China.
Methods:
180 subjects with venous thrombosis and 103 normal controls were tested for PC activity and subjected to PCR to amply PROC two regions for genetic analyses for PROC promoter polymorphisms and the p.Lys193del mutation, respectively.
Results:
PC activity in the deep venous thrombosis patients was 65.52±25.94, and 111.70±19.98 for the normal control population (P<0.05). Through we found 13 cases of p.Lys193del mutation in the DTV patients, with a positive rate of 7.2%, while in normal controls we detected 1 heterozygous mutation of p.Lys193del, and a positive rate of only 0.97%. This difference in mutation frequency was statistically significantly different between the two group (P<0.05). In the PROC promoter region, the distribution of alleles -1654 C,-1641 G, -1476 T in deep venous thrombosis patients and normal controls was significantly different (P<0.05). The genotype homozygous for the -1654C/C, -1641gG/G, -1476T/T, difference frequencies between the two groups was statistically significant (P<0.05).
Conclusions:
PROC gene p.Lys193del mutation accounts for about 7.2% of the incidence of venous thrombosis in Chinese people. The genotype homozygous for the -1654C/C, -1641gG/G, -1476T/T, can also be considered a risk factor of DVT in Chinese People.
Development and application of the TRV-induced gene-silencing system in different Rhododendron species
Authors: Xu Yunyun , Cui Yumeng , Chen Hanyu , Pu Ya , Zhang Chunying , Huang He 
Published: January 2024

Abstract

Rhododendron is one of the top ten traditional flowers in China, with high ornamental and medicinal values. However, molecular research on this species has been impeded by the lack of efficient molecular genetic techniques. Virus-induced gene silencing (VIGS) technology is an effective tool for analyzing gene functions, which has been successfully applied to many plant species. But there have been no reports of VIGS system for Rhododendron. In this study, tobacco rattle virus (TRV) was chosen to infect Rhododendron micranthum using phytoene desaturase (PDS) as the reporter gene. After the inoculation of pTRV2-RmPDS by leaf injection, photobleaching phenomena appeared in the newly developed leaves and the qRT-PCR assay demonstrated that RmPDS was successfully silenced. Then three parameters including the developmental stage, the Agrobacterium concentration and the inoculation temperature were examined to optimize the system. The silencing efficiency was increased from 2.4% to 11.4% and the optimized conditions were as follows: the developmental stage of the two true-leaf stage, the adjustment of the inoculation solution to a final OD600=1.5 and the inoculation temperature of 18 ℃. To further validate the system, the most optimal combination was used to infect other six rhododendron genotypes. R. mucronulatum, R. ovatum, R. × pulchrum, R. simsii and R. yedoense displayed the silenced phenotype of PDS as expected. We successfully established TRV-mediated VIGS technology in Rhododendron which could evaluate and characterize the function of plant genes without the need for cumbersome tissue culture.
Research on the dynamic characteristics of hydrodynamic bearing subjected to base shock loads
Authors: Huang Shizhang , Chen Feng , Chen Yong 
Published: January 2024

Abstract
The safety of hydrodynamic bearings can be significantly affected by base shock loads. This study focuses on investigating the shock dynamic characteristics of hydrodynamic bearings by combining the Euler equations, transient average Reynolds equation, and the asperity contact model. A tribo-dynamic model of hydrodynamic bearings under base shock load is established in this research. To validate the model, the corresponding drop shock tests are conducted on the hydrodynamic bearings, and the experimental and computational results show good agreement, confirming the reliability of the dynamic model. Building upon this foundation, a series of numerical simulations are performed to analyze the influence of various factors on the shock response of hydrodynamic bearings. The results indicate that the shock stiffness of hydrodynamic bearings gradually increases due to base shock loads. The anti-shock resistance capability of hydrodynamic bearings exhibits anisotropic behavior. Additionally, the shock stiffness also exhibits a nonlinear increase with the rotating speed. These findings contribute to a better understanding of the shock response of hydrodynamic bearings and provide valuable insights for designing hydrodynamic bearings with enhanced anti-shock resistance.
Preoperative Risk Factors for 90-day Postoperative Mortality Outcome in Patients With Non-occlusive Mesenteric Ischemia
Authors: Uchino Makoto , Kitaguchi Daichi , Furuya Kinji , Owada Yohei , Ogawa Koichi , Ohara Yusuke , Akashi Yoshimasa , Hashimoto Shinji , Enomoto Tsuyoshi , Oda Tatsuya 
Published: January 2024

Abstract
Purpose
The mortality rate of non-occlusive mesenteric ischemia remains despite surviving the acute postoperative period with tremendous treatment efforts, including emergency surgery, which is challenging. This study explored the preoperative risk factors for 90-day postoperative mortality in patients with non-occlusive mesenteric ischemia.
Methods
This was a single-center retrospective cohort study. Patients diagnosed with non-occlusive mesenteric ischemia who underwent emergency surgery between August 2014 and January 2023 were included in this study. All patients were divided into survival-to-discharge and mortality outcome groups at the 90-day postoperative follow-up. Preoperative factors, including co-morbidities, preoperative status of vital signs and consciousness, blood gas analysis, blood test results, and computed tomography, were compared between the two groups.
Results
Twenty patients were eligible for this study, and 90-day mortality was observed in 10 patients (50%). The mortality outcome group had significantly lower HCO3- (20.9 vs. 14.6, p = 0.006) and higher lactate (4.4 vs. 9.4, p = 0.023) compared with the survival outcome group. The median postoperative time to death was 19 [2–69] days, and five patients (50%) died after postoperative day 30, mainly because hemodialysis was discontinued due to hemodynamic instability in patients requiring hemodialysis.
Conclusion
Low preoperative HCO3- and high lactate levels may be preoperative risk factors for 90-day postoperative mortality in patients with non-occlusive mesenteric ischemia. However, patients on hemodialysis die from discontinuing hemodialysis even after surviving the acute postoperative phase. Hence, indications for emergency surgery in patients with risk factors for postoperative mortality should be carefully determined.
Study on the failure mechanism of high-temperature granite under two cooling modes
Authors: Li Chun , Zhang Chunwang , Hu Yaoqing , Meng Tao , Feng Gan 
Published: January 2024

Abstract
In the geothermal development of hot dry rock (HDR), both the drilling of the wellbore and the heat exchange of the heat reservoir involve the effects of different cold and hot conditions on the high-temperature rock mass. The testing machine for rock mechanics was used to conduct a uniaxial compression test and carry out micro testing on the treated samples; furthermore, with the help of scanning electron microscopy the fracture mechanism of granite subjected to different temperatures and cooling methods was studied. The results show: (1) With the gradual increase in temperature, the compressive strength of granite under the two cooling methods gradually decreases. (2) The failure modes of the samples under the two cooling methods are mainly shear failure of the "Y" type. The degree of damage of the sample under water cooling is significantly greater than that under natural cooling. Electron micrographs could confirm these results. (3)It can be obtained by testing the mineral composition and element changes of granite at different temperatures. When the temperature reaches 600℃, its change is more pronounced. The results of this study can provide a theoretical reference for the failure of the wellbore and the degree of fracture of the thermal reservoir rock mass during geothermal development.
A modified Green-Naghdi fractional order model and its application to thermoelectric semi-space heated by a moving heat source
Authors: Hassaballah Abaker A. , Hendy Mohamed , Ezzat Magdy 
Published: January 2024

Abstract
Classical Green-Naghdi (GN-II) model may have trouble accurately forecasting the thermo-mechanical behavior of electro-thermoelastic materials if memory-dependent impact is not taken into account. As results, we shall construct a new mathematical model of the Green-Naghdi (GN-II) theory, based on fractional order of heat transfer. A few theories are offered as limit examples. The method of the matrix exponential, which constitutes the basis of the state-space approach of modern theory, is used. Laplace transforms are utilized. The formulation is applied to half-space problem exposed to a uniform magnetic field and heated by a moving heat source with a constant speed. A numerical method is employed for the inversion of the Laplace transforms. The effects of several estimates of the figure-of-merit quantity, heat source speed, fractional parameter, magnetic number, and thermal shock parameter are considered.
Examining the challenges and opportunities of talent acquisition and recruitment in urban retail stores
Authors: Anika Afrin 
Published: January 2024

Abstract
The urban retail industry in Bangladesh has experienced significant growth, contributing to the overall economic development of the region. To remain competitive in this dynamic and fast-paced sector, urban retail stores must focus on attracting and retaining talented employees who can contribute to their success. This research explores the challenges and opportunities associated with talent acquisition and recruitment in urban retail stores in Bangladesh. The study was conducted using a questionnaire-based survey, with a sample size of 120 participants comprising employees, HR managers, and recruitment specialists from various urban retail stores. The survey covered important aspects such as recruitment processes, challenges faced in attracting top talent, and strategies employed to improve talent acquisition. Findings from the research highlight several critical challenges faced by urban retail stores in talent acquisition. The competitive labor market and the perception of the retail industry as a low-paying and high-stress environment hinder the attraction of skilled candidates. High turnover rates in the retail sector lead to increased recruitment costs and reduced organizational productivity. Moreover, the rapid changes in consumer preferences and the demand for digital skills present additional obstacles in recruiting candidates with the right skill set. Based on the research findings, the study proposes a set of actionable recommendations for urban retail stores to optimize talent acquisition efforts. Enhancing employer branding, offering competitive compensation and benefits packages, investing in employee training and development, leveraging technology for recruitment, and collaborating with educational institutions are among the key strategies suggested to attract and retain top talent. Additionally, offering flexible work arrangements and emphasizing opportunities for professional growth can appeal to potential candidates seeking stable and fulfilling careers. Implementing these recommendations can help urban retail stores build a skilled and committed workforce, gain a competitive edge in the labor market, and ensure long-term success in the dynamic retail industry of Bangladesh. The research underscores the critical importance of effective talent acquisition and recruitment strategies for the success and competitiveness of urban retail stores. By adopting innovative and proactive approaches to talent acquisition, retail establishments can address challenges, improve employee retention, and foster a positive work environment that attracts skilled employees. The study's insights provide valuable guidance for retail store managers, HR professionals, and stakeholders to make informed decisions in talent acquisition, contributing to the sustained growth and profitability of urban retail stores in Bangladesh.
Efficacy and prognosis of different chemotherapy frameworks in neoadjuvant treatment for HER2-positive breast cancer under targeted therapy：a real-world retrospective observational study
Authors: He Pengfei , Kang Wenli , Gao Fei , Wang Huibo , Zhang Kejie , Zhao Ziqi , Zhang Ruifeng , Huang Xiatian , Zhang Guoqiang , Shan Ming 
Published: January 2024

Abstract
Purpose
The optimal neoadjuvant chemotherapy regimen for early-stage human epidermal growth factor receptor 2(HER2)-positive breast cancer in the context of anti-HER2 therapy is unclear. The aim of this study was to compare the effects of anthracycline and non-anthracycline regimens, under different HER2 blocking conditions, on the efficacy and long-term prognosis of neoadjuvant chemotherapy for HER2-positive breast cancer.
Methods
In this real-world retrospective observational study, 270 patients treated with neoadjuvant therapy for stage II-III HER2-positive breast cancer in our center, were analyzed. All of these patients received neoadjuvant chemotherapy with anthracyclines or non-anthracyclines in the context of anti-HER2 therapy.
Results
Between 2016 and 2022, 270 patients met the inclusion criteria. The median follow-up time was 36.6 months (range 12.5–85.8 months). Of the 124 patients in the anthracycline group, 60 (48.4%, 95% CI 39.5–57.3) achieved pCR. 84 (57.5%, 95% CI 49.4–65.6) of the 146 patients in the non-anthracycline group achieved pCR (p = 0.133). Under single-target therapy, the pCR in anthracycline group and non-anthracycline group were 44.8% and 53.8%, respectively (P = 0.216). Under dual-target therapy, the pCR rates were 60.7% and 63.6% for both (p = 0.795). In the non-anthracycline treatment group under dual-target therapy, the pCR was 81.8% and 51.5% for platinum-containing and platinum-free regimens, respectively (p = 0.022). It was more pronounced in hormone receptor(HR)-positive patients. Multivariate analysis showed that HR status༈OR: 1.810;95%CI1.090-3.005༛p = 0.022༉, choice of targeted therapy༈OR༚1.883༛95%CI1.072-3.306༛p = 0.028༉and HER2 status༈OR༚4.216༛95%CI1.459-12.188༛p = 0.008༉were independent predictors of pCR. The estimated 3-year DFS rates were 93.5% and 85.9% in the non-anthracycline and anthracycline groups, respectively (p = 0.029). The 3-year OS was 96.1% and 93.3%, respectively (p = 0.118). The 3-year DFS in the non-anthracycline and anthracycline groups of patients with RCB 2 was 94.7% and 76.5%, respectively (p = 0.037). Especially in non-pCR patients treated with dual target therapy, patients treated with non-anthracyclines had better DFS benefit (p = 0.033). Patients who achieved pCR had better DFS (95% vs 85.9%; p = 0.001) and OS (97.2% vs 93.4%; p = 0.013) than those who did not.
Conclusion
In the neoadjuvant therapy of HER2-positive early-stage breast cancer patients, the use of platinum-containing non-anthracycline chemotherapy in the context of targeted therapy is an effective regimen for improving the long-term prognosis of patients. (particularly the long-term prognosis of non-pCR patients). In particular, patients with HR-positive breast cancer have better benefits under dual-target therapy.
LIA-V2X: A Lightweight identity mutual and batch authentication protocol for V2X Communication in Internet of Vehicles
Authors: liu Jiaxing , Xu Shengwei , liu Runsheng , Tian Yu , Zhu Xiaopeng , Xie Jianguo 
Published: January 2024

Abstract
Vehicular Ad hoc Networks (VANETs) hold significant importance within the domain of the Internet of Things. However, they face a critical issue concerning security threats. Criminals use open channels to eavesdrop, and tamper with messages to lock and control vehicles, which can lead to safety issues such as car accidents and privacy leaks. Most current approaches fail to adequately address the challenges of low-cost vehicles, anonymity, traceability, and revocation. Hence, we have developed LIA-V2X, a lightweight identity authentication scheme based on SM2 public key cryptography algorithm based on 256-bit ECC. It incorporates ECDH (Elliptic Curve Diffie-Hellman) key agreement and anonymous authentication techniques, enabling batch processing when multiple vehicles simultaneously enter a specific area, which can enhance communication efficiency. Furthermore, we have conducted both formal security analysis and informal security analysis utilizing the real number or random (RoR) oracle model to validate the security of our scheme in countering various malicious attacks within the IoV environment. Compared to existing identity authentication schemes for IoV, our scheme demonstrates improved performance in function and security features.
Ototoxic and Nephrotoxic Drugs in Neonatal Intensive Care Units: Results of a Spanish and Italian Survey
Authors: Arribas Cristina , Decembrino Nunzia , Raffaeli Genny , Amodeo Ilaria , González-Caballero Juan Luis , Riaza Mónica , Ortiz-Movilla Roberto , Massenzi Luca , Gizzi Camilla , Araimo Gabriella , Cattarelli Donatella , Aversa Salvatore , Martinelli Stefano , Frezza Simonetta , Orfeo Luigi , Mosca Fabio , Cavallaro Giacomo , Garrido Felipe 
Published: January 2024

Abstract
Background. Neonates face heightened susceptibility to drug toxicity, often exposed to off-label medications with dosages extrapolated from adult or pediatric studies. Premature infants in Neonatal Intensive Care Units (NICUs) are particularly at risk due to underdeveloped pharmacokinetics and exposure to multiple drugs. The study aimed to survey commonly used medications with a higher risk of ototoxicity and nephrotoxicity in Spanish and Italian NICUs.
Methods. A prospective cross-sectional study was conducted in Italian and Spanish NICUs using a web-based survey with 43 questions. A modified Delphi method involved experts refining the survey through online consensus. Ethical approval was obtained, and responses were collected from January to July 2023. The survey covered various aspects, including drug-related ototoxic and nephrotoxic management, hearing screening, and therapeutic drug monitoring.
Results. Responses from 131 participants (35.9% from Spain and 64.1% from Italy) revealed awareness of drug toxicity risks. Varied practices were observed in hearing screening protocols, and a high prevalence of ototoxic and nephrotoxic drug use, including aminoglycosides (100%), vancomycin (70.2%), loop diuretics (63.4%), and ibuprofen (62.6%). Discrepancies existed in guideline availability and adherence, with differences between Italy and Spain in therapeutic drug monitoring practices.
Conclusions. The study underscores the need for clinical guidelines and uniform practices in managing ototoxic and nephrotoxic drugs in NICUs. Awareness is high, but inconsistencies in practices indicate a necessity for standardization, including the implementation of therapeutic drug monitoring and the involvement of clinical pharmacologists. Addressing these issues is crucial for optimizing neonatal care in Southern Europe.
Discovery of immunotherapy targets for pediatric solid and brain tumors by exon-level expression
Authors: Shaw Timothy , Wagner Jessica , Tian Liqing , Wickman Elizabeth , Poudel Suresh , Wang Jian , Paul Robin , Koo Selene , Lu Meifen , Sheppard Heather , Fan Yiping , O’Neil Francis , Lau Ching , Zhou Xin , Zhang Jinghui , Gottschalk Stephen 
Published: January 2024

Abstract

Immunotherapy with CAR T cells for pediatric solid and brain tumors is constrained by available targetable antigens. Cancer-specific exons (CSE) present a promising reservoir of targets; however, these have not been explored and validated systematically in a pan-cancer fashion. To identify CSE targets, we analyzed 1,532 RNA-seq datasets from 16 types of pediatric solid and brain tumors for comparison with normal tissues using a newly developed workflow. We found 2,933 exons in 157 genes encoding proteins of the surfaceome or matrisome with high cancer specificity either at the gene (n=148) or the alternatively spliced (AS) isoform (n=9) level. Expression of selected AS targets, including the EDB domain of FN1 (EDB), and gene targets, such as COL11A1, were validated in pediatric PDX tumors. We generated CAR T cells specific to EDB or COL11A1 and demonstrated that COL11A1-CAR T-cells have potent antitumor activity. The full target list, explorable via an interactive web portal (https://cseminer.stjude.org/), provides a rich resource for developing immunotherapy of pediatric solid and brain tumors using gene or AS targets with high expression specificity in cancer.
Assessing the causal relationship between inflammatory cytokines and myasthenia gravis: A bidirectional and two-sample Mendelian randomization study
Authors: Wang Wenqiao , Li Min , Zhang Zengshan 
Published: January 2024

Abstract
Background:
Myasthenia gravis (MG) is categorized as an autoimmune disorder. A myriad of preceding investigations have elucidated the interrelation between inflammation and MG. Nevertheless, the precise array of inflammatory cytokines and their underlying mechanisms of action remain enigmatic.
Objective:
The primary intent of this research was to discern and elucidate the potential causal associations between 41 inflammatory cytokines and MG, employing a bidirectional and two-sample Mendelian randomization (MR) paradigm.
Methods:
Summary statistics pertaining to MG were procured from a genome-wide association study (GWAS) comprising 232 cases and 217,056 controls of European descent. Concurrently, genetic variants were sourced from an avant-garde GWAS centered on inflammatory cytokines, with an inclusive sample of 8,293 European subjects. An array of techniques including Inverse Variance Weighted (IVW), MR-Egger, weighted median, simple mode, and Weighted Mode were harnessed to assess the putative causal interlinkages between the 41 inflammatory cytokines and MG. Primarily, IVW outcomes formed the basis for deliberations on causality. Additionally, the MR-Egger approach was utilized to ascertain the potential horizontal pleiotropy of instrumental variables, and Cochran's Q statistics were employed to gauge the heterogeneity intrinsic to instrumental variables (IV).
Results:
Findings derived from the IVW model posited that IL-10 (OR=0.46, 95% CI=0.300.72, P=0.0006), IL-17 (OR=0.43, 95% CI=0.210.85, P=0.015), MIP1a (OR=2.24, 95% CI=1.014.97, P=0.048), MIP1b (OR=0.84, 95% CI=0.720.99, P=0.033), and SDF1a (OR=2.32, 95% CI=1.124.81, P=0.024) are potentially implicated in MG susceptibility. Concurrently, cytokines such as bNGF (OR=1.03, 95% CI=1.001.07, P=0.038, PFDR=0.778) and MIF (OR=0.97, 95% CI=0.95~1.00, P=0.033, PFDR=0.778) are ostensibly resultant sequelae of MG pathology. Upon the application of the False Discovery Rate (FDR) correction, a profound association was discerned solely for IL-10 in relation to MG (PFDR=0.025). Contrastingly, multiple inflammatory cytokines, including IL-17, MIP1a, MIP1b, and SDF1a, failed to meet the threshold post-FDR correction (with PFDR＞0.05), as did bNGF and MIF.
Conclusions:
The Mendelian randomization study, anchored in genetic variation methodologies, substantiates that IL-10 potentially holds significant relevance in the etiological landscape of MG. Contrarily, no discernible evidence was found to implicate other inflammatory cytokines in the post-onset progression of MG.