bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learning about simulated adversaries from human defenders using interactive cyber-defense games
Authors: Prebot Baptiste , Du Yinuo , Gonzalez Cleotilde 
Published: October 2023

AbstractGiven the increase in cybercrime, cybersecurity analysts (i.e. defenders) are in high demand. Defenders must monitor an organization’s network to evaluate threats and potential breaches into the network. Adversary simulation is commonly used to test defenders’ performance against known threats to organizations. However, it is unclear how effective this training process is in preparing defenders for this highly demanding job. In this paper, we demonstrate how to use adversarial algorithms to investigate defenders’ learning using interactive cyber-defense games. We created an Interactive Defense Game (IDG) that represents a cyber-defense scenario, which requires monitoring of incoming network alerts and allows a defender to analyze, remove, and restore services based on the events observed in a network. The participants in our study faced one of two types of simulated adversaries. A Beeline adversary is a fast, targeted, and informed attacker; and a Meander adversary is a slow attacker that wanders the network until it finds the right target to exploit. Our results suggest that although human defenders have more difficulty to stop the Beeline adversary initially, they were able to learn to stop this adversary by taking advantage of their attack strategy. Participants who played against the Beeline adversary learned to anticipate the adversary’s actions and took more proactive actions, while decreasing their reactive actions. These findings have implications for understanding how to help cybersecurity analysts speed up their training.
Interatrial block as a first clinical presentation of atrial cardiomyopathy related to a novel LMNA variant: a case report
Authors: Iavarone Michele , Covino Simona , Petillo Roberta , Russo Vincenzo 
Published: October 2023

Abstract

Background
Interatrial block (IAB) is a conduction delay in Bachmann’s bundle with a well-described association with structural heart disease, supraventricular arrhythmias, and cardiovascular events.

Case summary
We report the case of an asymptomatic 35-year-old man in whom the presence of IAB at electrocardiogram led to a comprehensive evaluation including speckle-tracking echocardiography, 24 h Holter monitoring, and genetic testing. Speckle-tracking echocardiography demonstrated a decrease in the longitudinal strain of interventricular septum, a typical feature of LMNA-related cardiomyopathy, and decreased indices of left atrial deformation. A diagnosis of cardiac laminopathy related to the frame shift variant c.1367 (p.Asn456Thrfs*24) of the LMNA gene was made. A dual-chamber implantable cardioverter defibrillator implantation was performed for the high risk of life-threatening ventricular tachyarrhythmias.

Discussion
This case demonstrates that IAB could be a rare presentation of a life-threatening laminopathy. Strain echocardiography is an essential tool to evaluate the deposition of fibrosis tissue in subclinical cardiomyopathies. Our report describes a novel variant of LMNA gene associated with a high risk of sudden cardiac death.
Long-term surgical outcomes of congenital supravalvular aortic stenosis: a systematic review, meta-analysis and microsimulation study
Authors: Meccanici Frederike , Notenboom Maximiliaan L , Meijssen Jade , Smit Vernon , van de Woestijne Pieter C , van den Bosch Annemien E , Helbing Willem A , Bogers Ad J J C , Takkenberg Johanna J M , Roos-Hesselink Jolien W 
Published: October 2023

Abstract

OBJECTIVES
Congenital supravalvular aortic stenosis (SVAS) is a rare form of congenital outflow tract obstruction and long-term outcomes are scarcely reported. This study aims to provide an overview of outcomes after surgical repair for congenital SVAS.

METHODS
A systematic review of published literature was conducted, including observational studies reporting long-term clinical outcome (&gt;2 years) after SVAS repair in children or adults considering &gt;20 patients. Early risks, late event rates and time-to-event data were pooled and entered into a microsimulation model to estimate 30-year outcomes. Life expectancy was compared to the age-, sex- and origin-matched general population.

RESULTS
Twenty-three publications were included, encompassing a total of 1472 patients (13 125 patient-years; pooled mean follow-up: 9.0 (6.2) years; median follow-up: 6.3 years). Pooled mean age at surgical repair was 4.7 (5.8) years and the most commonly used surgical technique was the single-patch repair (43.6%). Pooled early mortality was 4.2% (95% confidence interval: 3.2–5.5%) and late mortality was 0.61% (95% CI: 0.45–0.83) per patient-year. Based on microsimulation, over a 30-year time horizon, it was estimated that an average patient with SVAS repair (mean age: 4.7 years) had an observed life expectancy that was 90.7% (95% credible interval: 90.0–91.6%) of expected life expectancy in the matched general population. The microsimulation-based 30-year risk of myocardial infarction was 8.1% (95% credible interval: 7.3–9.9%) and reintervention 31.3% (95% credible interval: 29.6–33.4%), of which 27.2% (95% credible interval: 25.8–29.1) due to repair dysfunction.

CONCLUSIONS
After surgical repair for SVAS, 30-year survival is lower than the matched-general-population survival and the lifetime risk of reintervention is considerable. Therefore, lifelong monitoring of the cardiovascular system and in particular residual stenosis and coronary obstruction is recommended.
Robot-assisted thoracoscopic surgery for mediastinal tumours in children: a single-centre retrospective study of 149 patients
Authors: Zeng Qi , Chen Chenghao , Zhang Na , Yu Jie , Yan Dong , Xu Changqi , Liu Dingyi , Zhang Qian , Zhang Xu 
Published: October 2023

Abstract

OBJECTIVES
The purpose of this retrospective study was to summarize our experience in performing robot-assisted thoracoscopic surgery (RATS) for mediastinal tumours in children to investigate its safety and feasibility.

METHODS
This retrospective study involved 149 patients with mediastinal tumours who were hospitalized in the Department of Thoracic Surgery of Beijing Children’s Hospital, Capital Medical University, and underwent RATS for tumour resection from March 2021 to November 2022. Information on patient age, weight, tumour size, surgical incision selection, operative time, intraoperative bleeding, intraoperative complications, length of hospital stay, rate of conversion to thoracotomy and follow-up conditions were summarized.

RESULTS
All 149 surgeries were successfully completed with no cases of mortality. There were 77 male and 72 female patients, with a mean age of 5.9 years (range: 6 months–16 years, 8 months) and a mean weight of 23.6 kg (8.0–72.0 kg). The mean maximum tumour diameter was 5.5 cm (2.0–12.0 cm), the mean operative time was 106.7 min (25.0–260.0 min), the mean intraoperative bleeding volume was 11.3 ml (1.0–400.0 ml) and the mean hospital stay was 7.2 days (4.0–14.0 days). All patients recovered well with no cases of tumour recurrence or mortality during the postoperative follow-up period (3–23 months).

CONCLUSIONS
RATS is safe and feasible to apply in children with mediastinal tumours who are &gt;6 months of age and weigh more than 8 kg in terms of short-term outcomes, but longer-term follow-up is needed to fully evaluate the benefits. For cases that are associated with greater surgical difficulty and risk, a comprehensive surgical plan should be fully prepared in advance of surgery.
Phenotypic and genomic changes during Turnip mosaic virus adaptation to Arabidopsis thaliana mutants lacking epigenetic regulatory factors
Authors: Ambrós Silvia , Olmo-Uceda María J , Corrêa Régis L , Elena Santiago F 
Published: October 2023

Abstract
In this study, we investigated how an emerging RNA virus evolves, interacts, and adapts to populations of a novel host species with defects in epigenetically controlled plant defense mechanisms. Mutations in epigenetic regulatory pathways would exert different effects on defense-response genes but also induce large-scale alterations in cellular physiology and homeostasis. To test whether these effects condition the emergence and subsequent adaptation of a viral pathogen, we have evolved five independent lineages of a naive turnip mosaic virus (TuMV) strain in a set of Arabidopsis thaliana genotypes carrying mutations that influence important elements of two main epigenetic pathways and compare the results with those obtained for viral lineages evolved in wild-type plants. All evolved lineages showed adaptation to the lack of epigenetically regulated responses through significant increases in infectivity, virulence, and viral load although the magnitude of the improvements strongly depended on the plant genotype. In early passages, these traits evolved more rapidly, but the rate of evolution flattened out in later ones. Viral load was positively correlated with different measures of virulence, though the strength of the associations changed from the ancestral to the evolved viruses. High-throughput sequencing was used to evaluate the viral diversity of each lineage, as well as characterizing the nature of fixed mutations, evolutionary convergences, and potential targets of TuMV adaptation. Within each lineage, we observed a net increase in genome-wide genetic diversity, with some instances where nonsynonymous alleles experienced a transient rise in abundance before being displaced by the ancestral allele. In agreement with previous studies, viral VPg protein has been shown as a key player in the adaptation process, even though no obvious association between fixed alleles and host genotype was found.
Evolutionary diversity of the control of the azole response by Tra1 across yeast species
Authors: Librais Gabriela Marsiglio Nunes , Jiang Yuwei , Razzaq Iqra , Brandl Christopher J , Shapiro Rebecca S , Lajoie Patrick 
Published: October 2023

Abstract
Tra1 is an essential coactivator protein of the yeast SAGA and NuA4 acetyltransferase complexes that regulate gene expression through multiple mechanisms including the acetylation of histone proteins. Tra1 is a pseudokinase of the PIKK family characterized by a C-terminal PI3K domain with no known kinase activity. However, mutations of specific arginine residues to glutamine in the PI3K domains (an allele termed tra1Q3) result in reduced growth and increased sensitivity to multiple stresses. In the opportunistic fungal pathogen Candida albicans, the tra1Q3 allele reduces pathogenicity and increases sensitivity to the echinocandin antifungal drug caspofungin, which disrupts the fungal cell wall. Here, we found that compromised Tra1 function, in contrast to what is seen with caspofungin, increases tolerance to the azole class of antifungal drugs, which inhibits ergosterol synthesis. In C. albicans, tra1Q3 increases the expression of genes linked to azole resistance, such as ERG11 and CDR1. CDR1 encodes a multidrug ABC transporter associated with efflux of multiple xenobiotics, including azoles. Consequently, cells carrying tra1Q3 show reduced intracellular accumulation of fluconazole. In contrast, a tra1Q3 Saccharomyces cerevisiae strain displayed opposite phenotypes: decreased tolerance to azole, decreased expression of the efflux pump PDR5, and increased intracellular accumulation of fluconazole. Therefore, our data provide evidence that Tra1 differentially regulates the antifungal response across yeast species.
Bibliometric evaluation of Journal of Analytical Toxicology as a scholarly publication according to the Web-of-Science citation database
Authors: Jones Alan Wayne 
Published: October 2023

Abstract
Soon approaching its 50th anniversary, Journal of Analytical Toxicology (JAT) is an international scholarly publication specializing in analytical and forensic aspects of toxicology. Science Citation Index (SCI) and Journal Citation Reports (JCR), both of which are part of the Web-of-Science (WOS) database, were used to make a bibliometric evaluation of JAT articles. Between 1977 (volume 1) and 2023 (volume 47), a total of  n = 4,785 items were published in JAT; the top-ten most highly cited articles and the most prolific authors were identified. Changes in the journal impact factor (JIF) were studied between 1997 and 2022, and this metric varied from a low of 1.24 (2006) to a high of 3.36 (2020).The most recent JIF (2022) dropped to 2.5 and the corresponding 5 year JIF was 2.6. JAT’s most highly cited article (590 cites) was a working group (SWGTOX) report dealing with standard practices for the validation of analytical methods in forensic toxicology laboratories. JAT published 62 articles each of which were cited over 100 times and the H-index for JAT was 89. The most prolific author of JAT articles was credited with 119 items, the first in 1980 (volume 4) and the latest in 2023 (volume 47). JAT articles were cited 4,537 times in 2022 by all journals in the JCR database, although 520 of these were self-citations (11.5%). Bibliometric methods are increasingly used to evaluate the published work of individual scientists, university departments, entire universities and whole countries. Highly cited articles are considered more influential and authoritative compared with papers that are seldom or never cited.
The antennular setation and sternal morphology of parastacid crayfishes, with a comparison across Astacidea (Decapoda)
Authors: Kawai Tadashi , Patoka Jiří 
Published: October 2023

Abstract
The crustacean infraorder Astacidea is represented by two freshwater (Astacoidea and Parastacoidea) and two marine superfamilies (Enoplometopoidea and Nephropoidea). The antennule setation and sternal morphology were examined in specimens of 1) Parastacoidea: Cherax destructor, C. quadricarinatus, and C. tenuimanus from the Australian mainland; Astacopsis franklinii from Tasmania; C. gherardii, C. monticola, C. peknyi, and C. snowden from New Guinea; Paranephrops planifrons from New Zealand; Parastacus brasiliensis from Brazil; P. pugnax from Chile; and Astacoides madagascarensis from Madagascar; 2) Astacoidea: Pacifastacus leniusculus from Japan (where it is non-native); Procambarus clarkii and P. virginalis from the aquarium trade; and Cambaroides dauricus, C. schrenckii, C. similis, and C. wladiwostockiensis from Russia; 3) Enoplometopoidea: Enoplometopus chacei from Japan; E. debelius and E. occidentalis from the aquarium trade; and 4) Nephropoidea: Homarus americanus from the USA fish market; Metanephrops neptunus from Taiwan; and Metanephrops japonicus, Nephropsis stewari, and Thaumastocheles japonicus from Japan. Antennular setation and sternal morphology were identified as key taxonomic characters to distinguish between the four Astacid superfamilies (Astacoidea, Parastacoidea, Enoplometopoidea, and Nephropoidea). The two new key characters are described and illustrated in detail.
Conjunctive degrees and cylinders
Authors: Chitaia Irakli , Omanadze Roland , Sorbi Andrea 
Published: October 2023

Abstract
In this article, we define and study the notion of a $(c,c_{1})$-cylinder, which turns out to be very useful instrument for investigating the relationships between conjunctive reducibility ($c$-reducibility) and its injective version $c_{1}$-reducibility. Using this notion, we prove the following results: (i) Neither hypersimple sets nor hemimaximal sets can be $(c,c_{1})$-cylinders; (ii) The $c$-degree of a noncomputable c.e. set contains either only one or infinitely many noncomputable $c_{1}$-degrees; (iii) the $c$-degree of either a hemimaximal set or a hypersimple set contains infinitely many noncomputable $c_{1}$-degrees.
Impact of clinical target volume margin reduction in glioblastoma patients treated with concurrent chemoradiation
Authors: Di Perri Dario , Hofstede David , Hartgerink Dianne , Terhaag Karin , Houben Ruud , Postma Alida A , Hoeben Ann , Anten Monique , Ackermans Linda , Compter Inge , Eekers Daniëlle B P 
Published: October 2023

Abstract

Background
Glioblastoma (GBM) is widely treated using large radiotherapy margins, resulting in substantial irradiation of the surrounding cerebral structures. In this context, the question arises whether these margins could be safely reduced. In 2018, clinical target volume (CTV) expansion was reduced in our institution from 20 to 15 mm around the gross target volume (GTV) (ie, the contrast-enhancing tumor/cavity). We sought to retrospectively analyze the impact of this reduction.

Methods
All adult patients with GBM treated between January 2015 and December 2020 with concurrent chemoradiation (60Gy/2Gy or 59.4Gy/1.8Gy) were analyzed. Patients treated using a 20 (CTV20, n = 57) or 15 mm (CTV15, n = 56) CTV margin were compared for target volumes, dose parameters to the surrounding organs, pattern of recurrence, and survival outcome.

Results
Mean GTV was similar in both groups (ie, CTV20: 39.7cm3; CTV15: 37.8cm3; P = .71). Mean CTV and PTV were reduced from 238.9cm3 to 176.7cm3 (P = .001) and from 292.6cm3 to 217.0cm3 (P &lt; .001), for CTV20 and CTV15, respectively. As a result, average brain mean dose (Dmean) was reduced from 25.2Gy to 21.0Gy (P = .002). Significantly lower values were also observed for left hippocampus Dmean, brainstem D0.03cc, cochleas Dmean, and pituitary Dmean. Pattern of recurrence was similar, as well as patient outcome, ie, median progression-free survival was 8.0 and 7.0 months (P = .80), and median overall survival was 11.0 and 14.0 months (P = .61) for CTV20 and CTV15, respectively.

Conclusions
In GBM patients treated with chemoradiation, reducing the CTV margin from 20 to 15 mm appears to be safe and offers the potential for less treatment toxicity.
The association between different sleep health dimensions and sex, age, education, circadian preference, and chronic insomnia: a representative population-based study
Authors: Bjorvatn Bjørn , Waage Siri , Pallesen Ståle , Buysse Daniel J , Saxvig Ingvild W 
Published: October 2023

Abstract

Objectives
The aims were to explore multidimensional sleep health and the different dimensions of sleep health in the adult Norwegian population in relation to sex, age, education, circadian preference, and chronic insomnia.

Methods
A representative sample of 1028 Norwegians, aged 18 + years completed a cross-sectional web-based survey. Sleep health was measured with the multidimensional RU_SATED scale, which assesses the dimensions of regularity, satisfaction, alertness, timing, efficiency, and duration. Insomnia was assessed with the Bergen Insomnia Scale. Data were analyzed with chi-square tests, t-tests, one-way ANOVAs, and regression analyses, as appropriate. Response rate was 33.5%.

Results
Sleep health was better in males, with increasing age, and with higher educational level, and was poorer in participants with evening preference and chronic insomnia, compared to their respective counterparts. When investigating the different sleep health dimensions, males scored better than females on satisfaction (adjusted odds ratio [aOR] = 0.69, 95% CI = 0.51 to 0.93), timing (aOR = 0.66, 95% CI = 0.49 to 0.88), and efficiency (aOR = 0.68, 95% CI = 0.52 to 0.89). Older age was associated with better scores on regularity and satisfaction, whereas young age was associated with better scores on alertness and duration. High educational level was associated with better scores on alertness, timing, and duration. Evening types scored worse than morning types on regularity (aOR = 0.27, 95% CI = 0.18 to 0.41), satisfaction (aOR = 0.37, 95% CI = 0.26 to 0.53), and timing (aOR = 0.36, 95% CI = 0.26 to 0.51). Participants with chronic insomnia scored worse than participants without insomnia on all six sleep health dimensions.

Conclusions
Sleep health differed significantly in relation to sex, age, education, circadian preference, and chronic insomnia. However, specific group differences were not equally evident in all sleep health dimensions.
Defining spatiotemporal gene modules in liver regeneration using Analytical Dynamic Visual Spatial Omics Representation (ADViSOR)
Authors: Singh-Varma Anya , Shah Ashti M. , Liu Silvia , Zamora Ruben , Monga Satdarshan P. , Vodovotz Yoram 
Published: October 2023

Background:
The liver is the only organ with the ability to regenerate following surgical or toxicant insults, and partial hepatectomy serves as an experimental model of liver regeneration (LR). Dynamic changes in gene expression occur from the periportal to pericentral regions of the liver following partial hepatectomy; thus, spatial transcriptomics, combined with a novel computational pipeline (ADViSOR [Analytic Dynamic Visual Spatial Omics Representation]), was employed to gain insights into the spatiotemporal molecular underpinnings of LR.

Methods:
ADViSOR, comprising Time-Interval Principal Component Analysis and sliding dynamic hypergraphs, was applied to spatial transcriptomics data on 100 genes assayed serially through LR, including key components of the Wnt/β-catenin pathway at critical timepoints after partial hepatectomy.

Results:
This computational pipeline identified key functional modules demonstrating cell signaling and cell-cell interactions, inferring shared regulatory mechanisms. Specifically, ADViSOR analysis suggested that macrophage-mediated inflammation is a critical component of early LR and confirmed prior studies showing that Ccnd1, a hepatocyte proliferative gene, is regulated by the Wnt/β-catenin pathway. These findings were subsequently validated through protein localization, which provided further confirmation and novel insights into the spatiotemporal changes in the Wnt/β-catenin pathway during LR.

Conclusions:
Thus, ADViSOR may yield novel insights in other complex, spatiotemporal contexts.
TEG-based transfusion protocol is associated with decreased blood product use without increased risk of hemoperitoneum
Authors: Bromfield Brittany , Tellez Roberto , Hughes Dempsey L. , Brown Rebecca , Andrzejewski Margaret , Bawa Aditi , Lin Fei-Pi , Tublin Mitchell , Triulzi Darrell , Ganoza Armando , Duarte-Rojo Andres 
Published: October 2023

Background:
Thromboelastography (TEG) informs the need for blood product transfusions to prevent procedural bleeding complications in patients with cirrhosis. We aimed to evaluate the impact of using a TEG-based transfusion protocol on blood product utilization before paracentesis and the post-paracentesis hemoperitoneum (PPH) incidence.

Methods:
We conducted an ambispective analysis of patients with cirrhosis who underwent paracentesis from 2017 to 2021. In May 2019, we enacted a TEG-based transfusion protocol to guide pre-paracentesis blood product use. Patients with platelets < 20,000 or international normalized ratio ≥ 4 underwent TEG and received blood products if r value > 10 min or MA <30 mm. Patients were divided into pre-TEG and post-TEG protocol cohorts based on the date of paracentesis. Pre-paracentesis blood product transfusions in the form of platelets, fresh frozen plasma, and cryoprecipitates were recorded. PPH was defined as a decrease in hemoglobin of ≥1 g and the presence of blood on diagnostic imaging and/or the need for therapeutic intervention.

Results:
A total of 483 patients underwent 1281 paracenteses. The main etiologies of cirrhosis were alcohol (43%) and NASH (25%), and the mean MELD-sodium was 22±6. Pre-TEG and post-TEG protocol cohort sizes were similar: 253 patients and 607 paracenteses versus 230 patients and 674 paracenteses. After TEG-protocol implementation, blood product transfusions decreased significantly (228 vs. 49 products, p<0.001) with associated cost savings. One patient in each cohort developed PPH.

Conclusion:
Implementation of a pre-paracentesis TEG-based transfusion protocol for patients with cirrhosis successfully resulted in decreased blood product use with no associated increase in incidence of PPH.