bluuebunny/crossref_metadata_2025_split huggingface dataset:
Native PLGA nanoparticles attenuate Aβ-seed induced tau aggregation under in vitro conditions: potential implication in Alzheimer’s disease pathology
Authors: Paul Pallabi Sil , Patel Tark , Cho Jae-Young , Yarahmady Allan , Khalili Aria , Semenchenko Valentyna , Wille Holger , Kulka Marianna , Mok Sue-Ann , Kar Satyabrata 
Published: January 2024

AbstractEvidence suggests that beta-amyloid (Aβ)-induced phosphorylation/aggregation of tau protein plays a critical role in the degeneration of neurons and development of Alzheimer’s disease (AD), the most common cause of dementia affecting the elderly population. Many studies have pursued a variety of small molecules, including nanoparticles conjugated with drugs to interfere with Aβ and/or tau aggregation/toxicity as an effective strategy for AD treatment. We reported earlier that FDA approved PLGA nanoparticles without any drug can attenuate Aβ aggregation/toxicity in cellular/animal models of AD. In this study, we evaluated the effects of native PLGA on Aβ seed-induced aggregation of tau protein using a variety of biophysical, structural and spectroscopic approaches. Our results show that Aβ1-42 seeds enhanced aggregation of tau protein in the presence and absence of heparin and the effect was attenuated by native PLGA nanoparticles. Interestingly, PLGA inhibited aggregation of both 4R and 3R tau isoforms involved in the formation of neurofibrillary tangles in AD brains. Furthermore, Aβ seed-induced tau aggregation in the presence of arachidonic acid was suppressed by native PLGA. Collectively, our results suggest that native PLGA nanoparticles can inhibit the Aβ seed-induced aggregation of different tau protein isoforms highlighting their therapeutic implication in the treatment of AD.
SIRT1 as a potential key regulator for mediating apoptosis in oropharyngeal cancer using cyclophosphamide and all-trans retinoic acid
Authors: Haggagy Mahitab G. , Ahmed Lamiaa A. , Sharaky Marwa , Elhefnawi Mahmoud M. , Omran Mervat M. 
Published: January 2024

AbstractAlthough cyclophosphamide (CTX) has been used for recurrent or metastatic head and neck cancers, resistance is usually expected. Thus, we conducted this study to examine the effect of adding all-trans retinoic acid (ATRA) to CTX, to increase efficacy of CTX and reduce the risk of resistance developed. In this study, we investigated the combined effect of ATRA and CTX on the expression of apoptotic and angiogenesis markers in oropharyngeal carcinoma cell line (NO3), and the possible involved mechanisms. ATRA and CTX in combination significantly inhibited the proliferation of NO3 cells. Lower dose of CTX in combination with ATRA exhibited significant cytotoxicity than that of CTX when used alone, implying lower expected toxicity. Results showed that ATRA and CTX modulated oxidative stress; increased NOx and MDA, reduced GSH, and mRNA expression of Cox-2, SIRT1 and AMPK. Apoptosis was induced through elevating mRNA expressions of Bax and PAR-4 and suppressing that of Bcl-xl and Bcl-2, parallel with increased caspases 3 and 9 and decreased VEGF, endothelin-1 and CTGF levels. The primal action of the combined regimen on inflammatory signaling highlights its impact on cell death in NO3 cell line which was mediated by oxidative stress associated with apoptosis and suppression of angiogenesis.
Effects of different alkylating agents on photoreceptor degeneration and proliferative response of Müller glia
Authors: Nomura-Komoike Kaori , Nishino Reiko , Fujieda Hiroki 
Published: January 2024

AbstractAnimal models for retinal degeneration are essential for elucidating its pathogenesis and developing new therapeutic strategies in humans. N-methyl-N-nitrosourea (MNU) has been extensively used to construct a photoreceptor-specific degeneration model, which has served to unveil the molecular process of photoreceptor degeneration as well as the mechanisms regulating the protective responses of remaining cells. Methyl methanesulphonate (MMS), also known to cause photoreceptor degeneration, is considered a good alternative to MNU due to its higher usability; however, detailed pathophysiological processes after MMS treatment remain uncharacterized. Here, we analyzed the time course of photoreceptor degeneration, Müller glial proliferation, and expression of secretory factors after MNU and MMS treatments in rats. While the timing of rod degeneration was similar between the treatments, we unexpectedly found that cones survived slightly longer after MMS treatment. Müller glia reentered the cell cycle at a similar timing after the two treatments; however, the G1/S transition occurred earlier after MMS treatment. Moreover, growth factors such as FGF2 and LIF were more highly upregulated in the MMS model. These data suggest that comparative analyses of the two injury models may be beneficial for understanding the complex regulatory mechanisms underlying the proliferative response of Müller glia.
Intrinsic motivation in a virtual reality mock crime affects participants’ willingness to invest more effort in deceptive interviews
Authors: Branson Isabella S. , Speelman Craig P. , Rogers Shane L. 
Published: January 2024

AbstractIn studies of investigative interviewing, it is not well understood how participant experience of mock-crime activities might affect participants’ desire to perform (well) in subsequent interviews. In this study, we utilized two immersive virtual reality mock-crimes to examine if participants’ intrinsic motivation (i.e., competence, autonomy, relatedness) while committing the virtual mock-crime affects their desire to perform well in interviews. We also examined if the self-reported feeling of presence during the virtual reality mock-crime is associated with participants’ intrinsic motivation. We found significant positive associations between presence and all intrinsic motivation variables in both truth and lie conditions. We also found that competence and relatedness significantly predicted the self-reported effort to perform well in interviews. We discuss these results in the context of prior literature and provide recommendations for researchers on the design of mock-crime experiences.
Association between sarcopenia and osteoarthritis among the US adults: a cross-sectional study
Authors: Peng Peng , Wu Jiawei , Fang Weihua , Tian Jiaqing , He Mincong , Xiao Fangjun , Lin Kun , Xu Xuemeng , He Wei , Liu Wengang , Wei Qiushi 
Published: January 2024

AbstractThe association between sarcopenia and OA still presents many uncertainties. We aimed to assess whether sarcopenia is associated with occurrence of OA in US adults. We conducted a cross-sectional study consisting of 11,456 participants from National Health and Nutrition Examination Survey 1999–2006. Sarcopenia was defined by a low muscle mass. The skeletal muscle index (SMI) was calculated as the appendicular skeletal muscle mass divided by body mass indexes (BMI) or body weight. OA status was assessed by using self-reported questionnaire. We evaluated the association between sarcopenia and OA using multivariate regression models. In addition, subgroup and interaction analysis were performed. Sarcopenia was associated with OA when it was defined by the BMI-adjusted SMI (OR = 1.23 [95% CI, 1.01, 1.51]; P = 0.038) and defined by the weight-adjusted SMI (OR = 1.30 [95% CI, 1.10, 1.55]; P = 0.003). Subgroup and interaction analysis found that the strongest positive association mainly exists in smoker (OR = 1.54 [95% CI, 1.21, 1.95], Pint = 0.006), and this association is not significant in other groups. In conclusion, we found that sarcopenia was associated with occurrence of OA. Subgroup analysis revealed that the association between sarcopenia and OA was more pronounced in smoker. Further well-designed prospective cohort studies are needed to assess our results.
Prodigiosin/celecoxib-loaded into zein/sodium caseinate nanoparticles as a potential therapy for triple negative breast cancer
Authors: Mohamed Wafaa A. , El-Nekhily Nefertiti A. , Mahmoud Hoda E. , Hussein Ahmed A. , Sabra Sally A. 
Published: January 2024

AbstractNowadays, breast cancer is considered one of the most upsetting malignancies among females. Encapsulation of celecoxib (CXB) and prodigiosin (PDG) into zein/sodium caseinate nanoparticles (NPs) produce homogenous and spherical nanoparticles with good encapsulation efficiencies (EE %) and bioavailability. In vitro cytotoxicity study conducted on human breast cancer MDA-MB-231 cell lines revealed that there was a significant decline in the IC50 for encapsulated drugs when compared to each drug alone or their free combination. In addition, results demonstrated that there is a synergism between CXB and PDG as their combination indices were 0.62251 and 0.15493, respectively. Moreover, results of scratch wound healing assay revealed enhanced antimigratory effect of free drugs and fabricated NPs in comparison to untreated cells. Furthermore, In vitro results manifested that formulated nanoparticles exhibited induction of apoptosis associated with reduced angiogenesis, proliferation, and inflammation. In conclusion, nanoencapsulation of multiple drugs into nanoparticles might be a promising approach to develop new therapies for the managing of triple negative breast cancer.
High isolation 16-port massive MIMO antenna based negative index metamaterial for 5G mm-wave applications
Authors: Musaed Alya Ali , Al-Bawri Samir Salem , Abdulkawi Wazie M. , Aljaloud Khaled , Yusoff Zubaida , Islam Mohammad Tariqul 
Published: January 2024

AbstractA 16-port massive Multiple-Input-Multiple-Output (mMIMO) antenna system featuring a high gain and efficiency is proposed for millimeter-wave applications. The antenna system consists of 64 elements with a total size of 17 λo × 2.5λo, concerning the lowest frequency. Each 2 × 2 (radiating patch) subarray is designed to operate within the 25.5–29 GHz frequency range. The antenna's performance in terms of isolation, gain, and efficiency has been significantly improved by utilizing the proposed unique double and epsilon negative (DNG/ENG) metamaterials. The array elements are positioned on top of a Rogers RT5880 substrate, with ENG metamaterial unit cells interposed in between to mitigate coupling effects. Additionally, the DNG metamaterial reflector is positioned at the rear of the antenna to boost the gain. As a result, the metamaterial-based mMIMO antenna offers lower measured isolation reaching 25 dB, a maximum gain of 20 dBi and an efficiency of up to 99%. To further analyze the performance of the MIMO antenna, the diversity gain and enveloped correlation coefficient are discussed in relation to the MIMO parameters.
The efficacy and safety of ultrasonic bone scalpel for removing retrovertebral osteophytes in anterior cervical discectomy and fusion: A retrospective study
Authors: Yao Zhi , Zhang Shishuang , Liu Weijun , Wei Mengcheng , Fang Weizhi , Li Qingbo , Cai Lei , Wang Zhengkun , Zhou Chuankun , Zhou Yichi 
Published: January 2024

AbstractIn this study, we present a novel surgical method that utilizes the ultrasonic bone scalpel (UBS) for the removal of large retrovertebral osteophytes in anterior cervical discectomy and fusion (ACDF) and evaluate its safety and efficacy in comparison to the traditional approach of using high-speed drill (HSD). A total of 56 patients who underwent ACDF for retrovertebral osteophytes were selected. We recorded patients' baseline information, operation time, intraoperative blood loss, complications, JOA and VAS scores, and other relevant data. The mean operation time and the mean intraoperative blood loss in the UBS group were less than those in the HSD group (P < 0.05). Although both groups exhibited considerable improvements in JOA and VAS scores following surgery, there was no statistically significant difference between the two groups (P > 0.05). Additionally, no significant disparities were found in bone graft fusion between the two groups at 6- and 12-months postsurgery. Notably, neither group exhibited complications such as dura tear or spinal cord injury. Our study found that the use of UBS reduced operative time, minimized surgical bleeding, and led to clinical outcomes comparable to HSD in ACDF. This technique offers an effective and safe method of removing large retrovertebral osteophytes.
A facile strategy for preparation of Fe3O4 magnetic nanoparticles using Cordia myxa leaf extract and investigating its adsorption activity in dye removal
Authors: Ghoohestani Elham , Samari Fayezeh , Homaei Ahmad , Yosuefinejad Saeed 
Published: January 2024

AbstractThis study demonstrates the successful, facile, and cost-effective preparation of magnetic Fe3O4 nanoparticles (MNPs) via green procedure using Cordia myxa leaf extracts for efficient adsorption of methylene blue (MB) as a model of organic pollutant. The formation of Fe3O4 NPs was confirmed by a range of spectroscopy and microscopy techniques including FT-IR, XRD, FE-SEM, TEM, EDS, VSM, TGA, and BET-BJH. The synthesized spherical nanoparticles had a high specific surface area of 115.07 m2/g with a mesoporous structure. The formed Fe3O4 MNPs exhibited superparamagnetic behavior with saturation magnetization of 49.48 emu/g. After characterization, the adsorptive performance of the synthesized MNPs toward MB was evaluated. To achieve the maximum removal efficiency, the effect of key parameters such as adsorbent dosage (MNPs), initial adsorbate concentration, pH, and contact time on the adsorption process was evaluated. A maximum adsorption capacity of 17.79 mg/g was obtained, after one-hour incubation at pH 7.5. From the pHPZC of 7.1 of the synthesized adsorbent, the electrostatic attraction between MB and Fe3O4 NPs plays an important role in the adsorption process. The adsorption experimental data showed the closest match with the pseudo-second-order kinetic and Langmuir isotherm. The prepared Fe3O4 NPs were easily recovered by an external magnet and could be reused several times. Therefore, the synthesized MNPs seem to be excellent adsorbents for the removal of MB from aqueous solution.
Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency
Authors: Eiman Matthew N. , Kumar Shailesh , Serrano Negron Yazmin L. , Tansey Terry R. , Harbison Susan T. 
Published: January 2024

AbstractSleep latency, the amount of time that it takes an individual to fall asleep, is a key indicator of sleep need. Sleep latency varies considerably both among and within species and is heritable, but lacks a comprehensive description of its underlying genetic network. Here we conduct a genome-wide association study of sleep latency. Using previously collected sleep and activity data on a wild-derived population of flies, we calculate sleep latency, confirming significant, heritable genetic variation for this complex trait. We identify 520 polymorphisms in 248 genes contributing to variability in sleep latency. Tests of mutations in 23 candidate genes and additional putative pan-neuronal knockdown of 9 of them implicated CG44153, Piezo, Proc-R and Rbp6 in sleep latency. Two large-effect mutations in the genes Proc-R and Piezo were further confirmed via genetic rescue. This work greatly enhances our understanding of the genetic factors that influence variation in sleep latency.
Partitioning surface wave propagation on reconfigurable porous plane
Authors: Chu Zhiyuan , Tong Kin-Fai , Wong Kai-Kit , Chae Chan-Byoung , Zhang Yangyang 
Published: January 2024

AbstractThis paper introduces a novel reconfigurable technique for partitioning the propagation of surface waves by utilizing a T-shaped structure and pathways established through the introduction of fluid metal or metal pins into evenly spaced cylindrical cavities within a porous surface wave platform. Notably, the co-printing of metal and dielectric materials via 3D printing is employed, resulting in an expedited fabrication process. Extensive 3D electromagnetic simulations and experimental investigations validate the proposed approach’s efficacy in achieving surface wave division while minimizing interference. The study encompasses an exploration of diverse power distribution ratios achievable within the distributed surface waves. Critical physical parameters of the T-junction are comprehensively examined, including partition depth, junction geometry, output port symmetry, and asymmetry. Additionally, the research delves into the frequency-dependent behaviours of asymmetric T-junctions and pathways. These findings establish the groundwork for adaptable architectures, facilitating concurrent communication among multiple devices within a unified surface wave communication network. This innovation holds potential to enhance various applications through improved communication capabilities.
Safely and autonomously cutting meat with a collaborative robot arm
Authors: Wright Ryan , Parekh Sagar , White Robin , Losey Dylan P. 
Published: January 2024

AbstractLabor shortages in the United States are impacting a number of industries including the meat processing sector. Collaborative technologies that work alongside humans while increasing production abilities may support the industry by enhancing automation and improving job quality. However, existing automation technologies used in the meat industry have limited collaboration potential, low flexibility, and high cost. The objective of this work was to explore the use of a robot arm to collaboratively work alongside a human and complete tasks performed in a meat processing facility. Toward this objective, we demonstrated proof-of-concept approaches to ensure human safety while exploring the capacity of the robot arm to perform example meat processing tasks. In support of human safety, we developed a knife instrumentation system to detect when the cutting implement comes into contact with meat within the collaborative space. To demonstrate the capability of the system to flexibly conduct a variety of basic meat processing tasks, we developed vision and control protocols to execute slicing, trimming, and cubing of pork loins. We also collected a subjective evaluation of the actions from experts within the U.S. meat processing industry. On average the experts rated the robot’s performance as adequate. Moreover, the experts generally preferred the cuts performed in collaboration with a human worker to cuts completed autonomously, highlighting the benefits of robotic technologies that assist human workers rather than replace them. Video demonstrations of our proposed framework can be found here:https://youtu.be/56mdHjjYMVc.
The role of environment on women’s perception about their STEM studies: observations from a Global South country
Authors: Tsakalerou Mariza , Perveen Asma , Ayapbergenov Alibek , Rysbekova Aida 
Published: January 2024

AbstractThe growing global demand for STEM professionals is not being met by the supply of new graduates, a supply that is characterised by a significant lag in the percentage of women pursuing STEM studies. Interestingly enough, the percentage of female applicants entering STEM majors has been increasing yet only a minority of them pursue, or complete, engineering programs. Several studies for the developed world have identified several environmental factors responsible for this phenomenon. The scarcity of engineering professionals is a handicapping factor for development, even for the most advanced countries of the Global South. The objective of this exploratory study is to examine whether the environmental factors identified in the international literature are sufficient to explain the asymmetry in selecting an engineering or a natural sciences career among female undergraduates in an exemplary Global South country, Kazakhstan. To this purpose, a multifaceted survey was conducted among the female students pursuing STEM majors in the premier Kazakhstani university in the academic year 2021–2022. This study utilized a Likert Scale questionnaire, ordinal logistic regression, and factor analysis to explore factors affecting female students. Data reliability was confirmed through Confirmatory Factor Analysis (CFA). The factor and regression analysis of the results obtained demonstrates that there is no discernible difference between the observations in the literature and the situation in Kazakhstan.
Demonstration of a terahertz coplanar-strip spoof-surface-plasmon-polariton low-pass filter
Authors: Haghighat Mohsen , Darcie Thomas , Smith Levi 
Published: January 2024

AbstractThere is a growing interest in spoof surface plasmon polariton (SSPP) structures at terahertz (THz) frequencies for applications such as filtering, sensing, and communications. However, to date, there are limited experiments that confirm SSPP characteristics at THz frequencies. The majority of literature focuses on simulation or verification by device scaling to Gigahertz (GHz) frequencies where standard vector network analyzers are readily available. This paper presents the first experimental verification of SSPP characteristics at THz frequencies in a guided wave system using coplanar strip (CPS) feedlines. Specifically, we design three SSPP structures with varying band-edge frequencies (1.04 THz, 0.63 THz, and 0.53 THz), then fabricate and verify the low-pass transmission characteristics using a modified THz-time-domain spectrometer (THz-TDS) system. We find strong agreement between simulation, theory, and experiment.
SF3B4 downregulation restrains lung adenocarcinoma tumorigenesis via 5′ alternative splicing of KAT2A
Authors: Qu Ailin , Han Bo , Hua Mengmeng , Wang Chune , Li Tao 
Published: January 2024

AbstractAberrant expression of splicing factors, including SF3B4, plays a vital role in lung adenocarcinoma (LUAD). However, the impact of SF3B4 in the progression of LUAD has not been studied well. Here, we demonstrated the effects of SF3B4 in LUAD via apoptosis, proliferation, migration assays, etc. Gene manipulations confirmed the role of SF3B4 via KAT2A. SF3B4 was found to promote LUAD growth. Further studies found that, upon SF3B4 knockdown in LUAD cells, an alternative splice site occurred at the 5′-UTR of KAT2A, which led to the downregulation of KAT2A at both RNA and protein levels. Furthermore, the decrease in KAT2A expression partially reversed the effect of SF3B4 in promoting tumorigenesis. The axis SF3B4/ KAT2A was identified as a significant player in LUAD progression, shedding light on the therapeutic development in LUAD.