bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct mailing of HPV self-sampling kits to older women non-participating in cervical screening in the Czech Republic
Authors: Ngo Ondřej , Chloupková Renata , Cibula David , Sláma Jiří , Mandelová Lucie , Hejduk Karel , Hajdúch Marián , Minka Petr , Koudeláková Vladimíra , Jaworek Hana , Trnková Markéta , Vaněk Peter , Dvořák Vladimír , Dušek Ladislav , Májek Ondřej 
Published: October 2023

ABSTRACTBackgroundA population-based cervical cancer screening programme is implemented in the Czech Republic. However, participation is insufficient among women over 50 years. This study aimed to estimate the potential improvement in participation through directly mailed HPV self-sampling kits (HPVssk) compared to standard invitation letters in women aged 50-65 non-participating in screening.MethodsThe study recruited 1,564 eligible women (no cervical cancer screening in the last 3 years or more, no previous treatment associated with cervical lesions or cervical cancer). Eight hundred women were mailed with an HPVssk (HPVssk group), and 764 women were sent a standard invitation letter (control group) inviting them to a routine screening (Pap test). The primary outcome was a comparison of the overall participation rate between study groups using a binominal regression model.ResultsThe participation rate in the HPVssk group was 13.4% (95% CI 11.2–15.9%; 7.4% of women returned the HPVssk and 6.0% attended gynaecological examination) and 5.0% (95% CI 3.6–6.8%) in the control group. Using the binominal regression model, the difference between the groups was estimated as 7.6% (95% CI 5.0-10.2%; p < 0.001). In the HPVssk group, 22% of women who returned HPVssk had a positive result and 70% of them underwent a follow-up examination.ConclusionsCompared to traditional invitation letters, the direct mailing of the HPVssk achieved a significantly higher participation rate, along with a notable HPV positivity rate among HPVssk responders. This approach offers a potentially viable method for engaging women who have not yet attended a cervical screening programme.
Enhanced Ca2+Binding to EF-Hands through Phosphorylation of Conserved Serine Residues Activates MpRBOHB and Chitin-Triggered ROS Production
Authors: Hashimoto Takafumi , Hashimoto Kenji , Shindo Hiroki , Tsuboyama Shoko , Miyakawa Takuya , Tanokura Masaru , Kuchitsu Kazuyuki 
Published: October 2023

AbstractNADPH oxidases/RBOHs catalyze apoplastic ROS production and act as key signaling nodes, integrating multiple signal transduction pathways regulating plant development and stress responses. Although RBOHs have been suggested to be activated by Ca2+binding and phosphorylation by various protein kinases, a mechanism linking Ca2+binding and phosphorylation in the activity regulation remained elusive. Chitin-triggered ROS production required cytosolic Ca2+elevation and Ca2+binding to MpRBOHB in a liverwortMarchantia polymorpha. Heterologous expression analysis of truncated variants revealed that a segment of the N-terminal cytosolic region highly conserved among land plant RBOHs encompassing the two EF-hand motifs is essential for the activation of MpRBOHB. Within the conserved regulatory domain, we have identified two Ser residues whose phosphorylation is critical for the activationin planta. Isothermal titration calorimetry analyses revealed that phosphorylation of the two Ser residues increased the Ca2+binding affinity of MpRBOHB, while Ca2+binding is indispensable for the activation, even if the two Ser residues are phosphorylated. Our findings shed light on a mechanism through which phosphorylation potentiates the Ca2+-dependent activation of MpRBOHB, emphasizing the pivotal role of Ca2+binding in mediating the Ca2+and phosphorylation-driven activation of MpRBOHB, which is likely to represent a fundamental mechanism conserved among land plant RBOHs.
Rapamycin and caspofungin show synergistic antifungal effects in caspofungin-susceptible and caspofungin-resistantCandidastrainsin vitro
Authors: Lefranc Maxime , Accoceberry Isabelle , Biteau Nicolas , Noël Thierry 
Published: October 2023

AbstractObjectivesCaspofungin is an echinocandin antifungal agent that inhibits synthesis of glucan required for the fungal cell wall. Resistance is mediated by mutation of Fks1 glucan synthase, among which S645P is the most common resistance-associated polymorphism. Rapamycin is a macrolide that inhibits the mechanistic target of rapamycin (mTOR) protein kinase activity. This study investigated the interaction between rapamycin and caspofungin in inhibiting the growth of wild typeCandida albicansand Fks1 S645P mutant clinical isolate and wild typeCandida lusitaniaeand genetically engineered isogenic strain with Fks1 S645P mutation at equivalent position.MethodsInteractions between caspofungin and rapamycin were evaluated using the microdilution checkerboard method in liquid medium. The results were analysed using the fractional inhibitory concentration (FIC) index and the response surface (RS) analysis according to the Bliss model.ResultsSynergy between rapamycin and caspofungin was shown forC. albicansandC. lusitaniaestrains by RS analysis of the checkerboard tests. Synergy was observed in strains sensitive and resistant to caspofungin. Weak subinhibitory concentrations of rapamycin were sufficient to restore caspofungin susceptibility.ConclusionsWe report here for the first time synergy between caspofungin and rapamycin inCandidaspecies. Synergy was shown for strains susceptible and resistant to caspofungin. This study highlights the role of the TOR pathway in sensing antifungal-mediated cell wall stress and in modulating the cellular response to echinocandins inCandidayeasts.
Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strain
Authors: Hunter Oarteze , Talkish Jason , Quick-Cleveland Jen , Igel Haller , Tan Asako , Kuersten Scott , Katzman Sol , Donohue John Paul , Jurica Melissa , Ares Manuel 
Published: October 2023

ABSTRACTIntron branch point (BP) recognition by the U2 snRNP is a critical step of splicing, vulnerable to recurrent cancer mutations and bacterial natural product inhibitors. The BP binds a conserved pocket in the SF3B1 (human) or Hsh155 (yeast) U2 snRNP protein. Amino acids that line this pocket affect binding of splicing inhibitors like Pladienolide-B (Plad-B), such that organisms differ in their sensitivity.To study the mechanism of splicing inhibitor action in a simplified system, we modified the naturally Plad-B resistant yeastSaccharomyces cerevisiaeby changing 14 amino acids in the Hsh155 BP pocket to those from human. This humanized yeast grows normally, and splicing is largely unaffected by the mutation. Splicing is inhibited within minutes after addition of Plad-B, and different introns appear inhibited to different extents. Intron-specific inhibition differences are also observed during co-transcriptional splicing in Plad-B using single-molecule intron tracking (SMIT) to minimize gene-specific transcription and decay rates that cloud estimates of inhibition by standard RNA-seq. Comparison of Plad-B intron sensitivities to those of the structurally distinct inhibitor Thailanstatin-A reveals intron-specific differences in sensitivity to different compounds. This work exposes a complex relationship between binding of different members of this class of inhibitors to the spliceosome and intron-specific rates of BP recognition and catalysis. Introns with variant BP sequences seem particularly sensitive, echoing observations from mammalian cells, where monitoring individual introns is complicated by multi-intron gene architecture and alternative splicing. The compact yeast system may hasten characterization of splicing inhibitors, accelerating improvements in selectivity and therapeutic efficacy.
Membrane potential dynamics unveil the promise of Bioelectrical Antimicrobial Susceptibility Testing (BeAST) for anti-fungal screening
Authors: de Souza-Guerreiro Tailise Carolina , Huan Bacellar Letícia , Santana da Costa Thyerre , Tasic Ljubica , Asally Munehiro 
Published: October 2023

AbstractMembrane potential is a useful marker for antimicrobial susceptibility testing (AST) due to the fundamental characteristic of vital cells. However, the difficulties associated with measuring the membrane potential in microbes restrict its broad application. In this study, we present Bioelectrical AST (BeAST) using the model fungusSaccharomyces cerevisiae. Using fluorescent indicators (DiBAC4(3), ThT and TMRM), we measured plasma and mitochondrial membrane-potential dynamics upon electric stimulation. We find that a 2.5-second electric stimulation induces hyperpolarisation of plasma membrane lasting 20 minutes in vitalS. cerevisiae, but depolarisation in inhibited cells. The numerical simulation of FitzHugh-Nagumo model successfully recapitulates vitality-dependent dynamics. The model also suggests that the magnitude of plasma-membrane potential dynamics (PMD) correlates with the degree of inhibition. To test this prediction and to examine if BeAST can be used for assessing novel anti-fungal compounds, we treat cells with biogenic silver nanoparticles (bioAgNPs) synthesised using orange fruit flavonoids and Fusarium oxysporum. Comparing BeAST with optical density assay with various stressors, we show that PMD correlates with the severityof growth inhibitions. These results suggest that BeAST holds promise for screening anti-fungal compounds, offering a valuable approach to tackling antimicrobial resistance.
The overlapping genetic architecture of psychiatric disorders and cortical brain structure
Authors: Sha Zhiqiang , Warrier Varun , Bethlehem Richard A.I. , Schultz Laura M. , Merikangas Alison , Sun Kevin Y. , Gur Ruben C. , Gur Raquel E. , Shinohara Russell T. , Seidlitz Jakob , Almasy Laura , Andreassen Ole A. , Alexander-Bloch Aaron F. 
Published: October 2023

AbstractBoth psychiatric vulnerability and cortical structure are shaped by the cumulative effect of common genetic variants across the genome. However, the shared genetic underpinnings between psychiatric disorders and brain structural phenotypes, such as thickness and surface area of the cerebral cortex, remains elusive. In this study, we employed pleiotropy-informed conjunctional false discovery rate analysis to investigate shared loci across genome-wide association scans of regional cortical thickness, surface area, and seven psychiatric disorders in approximately 700,000 individuals of European ancestry. Aggregating regional measures, we identified 50 genetic loci shared between psychiatric disorders and surface area, as well as 26 genetic loci shared with cortical thickness. Risk alleles exhibited bidirectional effects on both cortical thickness and surface area, such that some risk alleles for each disorder increased regional brain size while other risk alleles decreased regional brain size. Due to bidirectional effects, in many cases we observed extensive pleiotropy between an imaging phenotype and a psychiatric disorder even in the absence of a significant genetic correlation between them. The impact of genetic risk for psychiatric disorders on regional brain structure did exhibit a consistent pattern across highly comorbid psychiatric disorders, with 80% of the genetic loci shared across multiple disorders displaying consistent directions of effect. Cortical patterning of genetic overlap revealed a hierarchical genetic architecture, with the association cortex and sensorimotor cortex representing two extremes of shared genetic influence on psychiatric disorders and brain structural variation. Integrating multi-scale functional annotations and transcriptomic profiles, we observed that shared genetic loci were enriched in active genomic regions, converged on neurobiological and metabolic pathways, and showed differential expression in postmortem brain tissue from individuals with psychiatric disorders. Cumulatively, these findings provide a significant advance in our understanding of the overlapping polygenic architecture between psychopathology and cortical brain structure.
DMC1 and RAD51 bind FxxA and FxPP motifs of BRCA2 via two separate interfaces
Authors: Miron Simona , Legrand Pierre , van Rossum-Fikkert Sari E. , Ristic Dejan , Badar Atifa , Kanaar Roland , Zinn-Justin Sophie , Zelensky Alex N. 
Published: October 2023

AbstractIn vertebrates, the BRCA2 protein is essential for meiotic and somatic homologous recombination (HR) due to its interaction with RAD51 and DMC1 strand exchange proteins (recombinases). The interaction is mediated by FxxA and FxPP motifs, whose defining feature is the invariant phenylalanine. The FxxA motifs, present in the eight BRC repeats in the central region of BRCA2, compete with the FxxA motif of the linker region of RAD51 that is responsible for recombinase self-oligomerization. In vitro, BRCs disrupt RAD51 nucleoprotein filaments, but they are essential for RAD51 function in the context of the full-length BRCA2 protein. The role of the FxPP motifs is poorly studied but they also contribute to BRCA2 function in cells. In particular, the C-terminal TR2/CTRB domain of BRCA2, which contains an FxPP motif, is required for stabilization of RAD51 filament and replication fork protection. We recently found that deletion of the BRCA2 PhePP domain, which contains another FxPP motif, disrupts DMC1 but not RAD51 function in meiosis. Here we provide a mechanistic explanation for this phenotype by solving the crystal structure of the complex between DMC1 and the PhePP domain of BRCA2. Our structure reveals that, despite sequence similarity, the A-motifs (FxxA) and P-motifs (FxPP) bind to distinct and contiguous sites on the recombinases. The PhePP P-motif binding site is mostly located at the ATPase domain surface of a DMC1 monomer, but also extends to the linker region of the adjacent monomer, thus engaging two adjacent protomers in the DMC1 oligomer. Our structural analysis provides a mechanism explaining how PhePP favors the formation of the DMC1 nucleoprotein filament and stabilizes it. It corroborates and explains the stabilizing effect of the P-motif from BRCA2 TR2/CTRB on RAD51.
Synergistic corrosion protection of stir-cast hybrid aluminum composites reinforcing CeO2 and GNPs nano-particulates
Authors: Kumar Dinesh , Singh Satnam , Angra Surjit 
Published: October 2023

Purpose
This study aims to investigate the corrosion behavior of stir-cast hybrid aluminum composite reinforced with CeO2 and graphene nanoplatelets (GNPs) nanoparticulates used as cylinder liner material in the engines (automotive, aerospace and aircraft industries).

Design/methodology/approach
The composites were prepared using the stir-casting technique, and their microstructure and corrosion behavior was evaluated using scanning electron microscopy (SEM) and potentiodynamic polarization test, respectively.

Findings
The results showed that the addition of CeO2 and GNPs improved the corrosion resistance of the composites, and the optimal combination of these two nanoparticles was found to be 3 wt.% CeO2 and 3 wt.% GNPs. The enhanced corrosion resistance was attributed to the formation of a protective layer on the surface of the composite, as well as the effective dispersion and uniform distribution of nanoparticles in the matrix. The 0.031362 was noted as the lowest corrosion rate (mmpy) and was noticed in 94% Al-6061 alloy + (3 Wt.% CeO2 + 3 Wt.% GNPs) sample at room temperature and at elevated temperatures; the corrosion rate (mmpy) was observed as 0.0601 and 0.0636 at 45 °C and 75 °C, respectively.

Originality/value
In the vast majority of the published research publications, either cerium oxide or graphene nanoplatelets were utilized as a single reinforcement or in conjunction with other types of reinforcement such as alumina, silicon carbide, carbon nano-tubes, tungsten carbide, etc., but on the combination of the CeO2 and GNPs as reinforcements have very less literatures with 2 wt.% each only. The prepared hybrid aluminum composite (reinforcing 1 wt.% to 3 wt.% in Al-6061 alloy) was considered for replacing the cylinder liner material in the piston-cylinder arrangement of engines.
Contextual marketing and information privacy concerns in m-commerce and their impact on consumer loyalty
Authors: Irgui Aya , Qmichchou Mohammed 
Published: October 2023

PurposeThis study examines the effect of contextual perceived value activated by contextual marketing offers and information privacy concerns on consumer loyalty in mobile commerce.Design/methodology/approachThe survey was conducted through 340 mobile users in Morocco and the collected data were analyzed using structural equation modeling.FindingsThis study's results show that contextual marketing and information privacy concerns are key determinants in improving customer loyalty in the m-commerce context. Perceived ubiquity has a positive impact on perceived trust, which also impacts consumer loyalty. Information privacy concerns also have a positive impact on customer satisfaction, yet it does not impact perceived trust, which is contrary to the results of other researchers. It can also be concluded that customer satisfaction and trust are important antecedents of consumer loyalty.Practical implicationsThis research gives rise to some important managerial and strategic implications in order to integrate contextual marketing strategies, as well as theoretical implications that concern this field of study.Originality/valueThis research makes a significant contribution to knowledge by examining the role of contextual marketing and information privacy concerns in the m-commerce context. These results will be considered useful for marketers and for businesses in general who wish to integrate a marketing strategy that is based on a customer-centric approach. It also contributes to the related literature, as there are few studies focused on m-commerce and contextual marketing within the context of Morocco.
The determinants of work - life balance practices in Spanish listed companies
Authors: Calzón-Menéndez Faustino , Sacristán-Navarro María , Cabeza-García Laura 
Published: October 2023

PurposeTo empirically study the determinant factors of adopting work–life balance measures at the firm level.Design/methodology/approachAfter reviewing the main theories that explain work–life balance practices, IBEX-35 Spanish companies during 2015–2019 are studied to see whether the firm sector, the presence of female employees, qualified personnel, a company agreement and an equality plan determine these types of practices.FindingsThe results indicate a negative relationship between the firm activity sector and adopting work–life balance practices. Industrial companies offer fewer work–life balance measures than service companies. A company agreement implies more extensive adoption of work-life practices. Older companies are characterized by greater adoption of work-life practices, in contrast to their level of profitability that is associated negatively with these practices.Practical implicationsSince it is difficult for industrial companies to change their idiosyncrasies, it is necessary to incorporate other measures that promote work–life balance, such as timetable flexibility. Companies and policymakers should promote the advantages of a company agreement to contribute to the implementation of work–life balance practices.Originality/valueThe empirical evidence is scarce, especially in Spain and at the company level, given that most previous studies have focused on workers and the Anglo-Saxon sphere. Given the growing interest in this topic in the business world, the authors intend to contribute to this scarce literature by incorporating variables considered in previous studies, as well as other more innovative ones (agreement, equality plan) in a single model through a longitudinal study.
Deglobalization: a systematic enquiry using 5 Ws and 1 H framework
Authors: Garg Shamita , Sushil Sushil 
Published: October 2023

PurposeThe world is on the verge of entering the deglobalization age, and industrialized economies have ushered it in. However, there is still a scarcity of comprehensive and rigorous studies in this field. This research has tried to analyze the evolution and characteristics of deglobalization research.Design/methodology/approachThe authors have employed bibliometric analysis for examining the existing evidence on accelerating deglobalization thinking based on a thorough analysis of articles published during a roughly 25-year span between 1996 and 2022. This study has used the TISM-P technique to study the relationship among the factors accelerating deglobalization thinking. This research reviews the articles on several dimensions of deglobalization using the “what”, “why”, “how”, “who”, “when” and “where” approaches.FindingsThe authors specify the critical factors, policy reforms, approaches and observed characteristics explored in this developing research area.Practical implicationsThe authors have analyzed the factors accountable for rising deglobalization thinking and also suggested strategic recommendations based on the findings to minimize the adverse impact of globalization.Originality/valueAlthough there is a wealth of literature on globalization, very little study has been done in the field of deglobalization. This is the first substantive review being done in the deglobalization domain. The contemporary research has used the bibliometric approach and the “5W and 1 H” framework to gain a comprehensive understanding of the changing paradigm.
What do you think of transparent pay? The reflexive thematic analysis of opinions about pay transparency in Poland
Authors: Kulikowski Konrad 
Published: October 2023

PurposeWith pay transparency (PT) as an emerging trend in organizations around the world and the European Union promoting open pay regulations, PT is of increasing interest to scientists, managers and policymakers. However, it is still unclear what people think about PT and what theoretical perspectives might explain people's views on transparent pay. Therefore, the purpose of this paper is to explore and systematize opinions about PT and to propose theoretical frameworks to understand different reactions to it.Design/methodology/approachQualitative reflexive thematic analysis of the Internet debate that emerged in Poland in 2019 in response to the proposition of new law regulations aimed to improve PT by introducing obligatory pay ranges in job offers.FindingsThe author's analysis revealed a set of 41 specific and often opposite opinions about PT, which allow for the systematization of hopes and concerns related to PT around the 7 favorable and 6 unfavorable higher-order themes.Social implicationsThe author's results might inform policymakers and managers about the possible risks and benefits of PT implementation. Revealed opposite opinions about PT raise awareness that PT policies, while solving some social problems, might simultaneously create others.Originality/valueThe author provides new insights into opinions that people hold about transparent pay based on real-world data. The author suggests theoretical perspectives for understanding and predicting reactions to PT, such as Hobfoll's Conservation of Resources Theory and Adam`s Equity Theory. Based on this, the authors propose that opposite opinions about PT might be explained by (a) perceived pay dispersion fairness and (b) perceived PT costs-benefits ratio.