bluuebunny/crossref_metadata_2025_split huggingface dataset:
Native lamin A/C proteomes and novel partners from heart and skeletal muscle in a mouse chronic inflammation model of human frailty
Authors: Elzamzami Fatima D. , Samal Arushi , Arun Adith S. , Dharmaraj Tejas , Prasad Neeti R. , Rendon-Jonguitud Alex , DeVine Lauren , Walston Jeremy D. , Cole Robert N. , Wilson Katherine L. 
Published: October 2023

Clinical frailty affects ∼10% of people over age 65 and is studied in a chronically inflamed (Interleukin-10 knockout; “IL10-KO”) mouse model. Frailty phenotypes overlap the spectrum of diseases (“laminopathies”) caused by mutations in LMNA. LMNA encodes nuclear intermediate filament proteins lamin A and lamin C (“lamin A/C”), important for tissue-specific signaling, metabolism and chromatin regulation. We hypothesized that wildtype lamin A/C associations with tissue-specific partners are perturbed by chronic inflammation, potentially contributing to dysfunction in frailty. To test this idea we immunoprecipitated native lamin A/C and associated proteins from skeletal muscle, hearts and brains of old (21–22 months) IL10-KO versus control C57Bl/6 female mice, and labeled with Tandem Mass Tags for identification and quantitation by mass spectrometry. We identified 502 candidate lamin-binding proteins from skeletal muscle, and 340 from heart, including 62 proteins identified in both tissues. Candidates included frailty phenotype-relevant proteins Perm1 and Fam210a, and nuclear membrane protein Tmem38a, required for muscle-specific genome organization. These and most other candidates were unaffected by IL10-KO, but still important as potential lamin A/C-binding proteins in native heart or muscle. A subset of candidates (21 in skeletal muscle, 30 in heart) showed significantly different lamin A/C-association in an IL10-KO tissue (p &lt; 0.05), including AldoA and Gins3 affected in heart, and Lmcd1 and Fabp4 affected in skeletal muscle. To screen for binding, eleven candidates plus prelamin A and emerin controls were arrayed as synthetic 20-mer peptides (7-residue stagger) and incubated with recombinant purified lamin A “tail” residues 385–646 under relatively stringent conditions. We detected strong lamin A binding to peptides solvent exposed in Lmcd1, AldoA, Perm1, and Tmem38a, and plausible binding to Csrp3 (muscle LIM protein). These results validated both proteomes as sources for native lamin A/C-binding proteins in heart and muscle, identified four candidate genes for Emery-Dreifuss muscular dystrophy (CSRP3, LMCD1, ALDOA, and PERM1), support a lamin A-interactive molecular role for Tmem38A, and supported the hypothesis that lamin A/C interactions with at least two partners (AldoA in heart, transcription factor Lmcd1 in muscle) are altered in the IL10-KO model of frailty.
Trends in exercise for hypertension: a bibliometric analysis
Authors: Lou Yan , Sun Ning , Zhang Min , Qiu Yongzhen , Wang Jie , Chen Jiajia 
Published: October 2023

ObjectiveTo investigate development trends and research hotspots of exercise for hypertension research and provide researchers with fresh perspectives for further studies.Materials and methodsArticles and reviews regarding exercise and hypertension spanning May 1st 2003 to May 18th 2023 were retrieved from the Web of Science Core Collection (WOSCC) database. VOSviewer and Citespace were mainly used to perform and visualize co-authorship, co-citation, and co-occurrence analysis of countries, institutions, authors, references and keywords in this field.ResultsA total of 1,643 peer-reviewed papers were identified, displaying a consistent increasing trend over time. The most prolific country and institution were Brazil and University of Sao Paulo, respectively. And we identified the most productive author was lrigoyen, Maria Claudia C, while Pescatello Linda S was the most co-cited author. Journal of hypertension was the most prominent journal, and Hypertension was the journal which was the most co-cited. And this field can be divided into 3 research themes: exercise interventions for hypertension, age-specific relevance of exercise for hypertension, and the global burden of hypertension and the role of exercise. According to the result of keywords analysis, epidemiological information, types of exercise, target population, mechanism, and study design are significant research areas. “Resistance training”, “adults”, and “heart rate variability” were identified as the major future research foci.ConclusionsThe findings offer a scientific insight into exercise for hypertension research, presenting researchers with valuable information to understand the current research status, hotspots, and emerging trends for future investigation.
Higher waist circumference is associated with increased likelihood of female infertility: NHANES 2017-2020 results
Authors: Yin Ying-Hua , Zhou Su-Yu , Lu Dong-Fang , Chen Xiu-Ping , Liu Bo , Lu Shan , Han Xiao-Dong , Wu Ai-Hua 
Published: October 2023

BackgroundWaist circumference can be used as an anthropometric measure to assess central obesity and is easier and more convenient than the waist-to-hip ratio in identifying the risk of obesity and medical problems. Most studies showing an association between obesity and infertility in women have used BMI to measure obesity. Our goal was to examine any potential association between waist circumference and infertility.MethodsThis cross-sectional study, which formed part of the National Health and Nutrition Examination Survey (NHANES), comprised women ages 18 to 45 between 2017 and 2020. Participants without waist circumference data or information on infertility were removed from the study. The independent relationship between waist circumference and infertility was investigated using weighted binary logistic regression and subgroup analysis.ResultsWe investigated 1509 participants and discovered that the prevalence of infertility rose as the WC trisection rose. (tertile 1, 7.55%; tertile 2, 10.56%; tertile 3, 15.28%; trend &lt; 0.001). Multivariate logistic regression showed that after total adjustment, higher WC levels were associated with an increased likelihood of infertility in women (OR1.02; 95% CI 1.01-1.03), and There was a 2% rise in the incidence of infertility for every unit (cm) increased WC. Subgroup analysis and interaction tests showed no significant dependence of the effects of marital status, diabetes, hypertension, and high cholesterol on the association between WC and infertility (p for all interaction tests &gt; 0.05). The inflection point of the positive non-linear relationship between WC and infertility was 116.6 cm.ConclusionExcessive waist circumference assessment may increase the probability of infertility, and more attention should be paid to the management of waist circumference should be given more attention.
Why don’t we consume energy more efficiently? a Lisbon Parish council case study
Authors: Barreto Ana Margarida , Correia de Brito Nuno , Boechat Anna Carolina , Oliveira Martins Luís , Martins Fernando 
Published: October 2023

Introduction: Almost 50% of the European Union’s final energy consumption is used for heating and cooling, 80% of which in buildings. The European Commission recently issued the “Efficiency Energy First Principle,” a formal recommendation to EU countries prioritizing energy efficiency measures over other energy-related investments. Decarbonizing the aging housing stock represents a significant challenge to Southern Europe and the remaining Member States. This exploratory research aims to understand why Portuguese people fail to increase their energy efficiency; it then proposes potential interventions. Several studies have looked into the effect of technology-based and behavior-based strategies (individual, socioeconomic and demographic, as well as contextual factors) regarding residential energy consumption. Few, however, have brought all these factors together in one project as in this case.Methods: We used the integrative COM-B model to investigate three core influences of behavior, namely, capability, opportunity, and motivation in a qualitative analysis of a sample of citizens of one specific Lisbon, Portugal community. The Behavior Change Wheel model was then used to propose interventions that might promote energy-responsible behavior.Results: Our finding suggests that investments in structural strategies, and, above all, in behavioral strategies are needed to achieve efficient residential electricity consumption. Specifically, we found a lack of capability (i.e., people’s physical skills and strength, knowledge, and regulation skills) represented the greatest barrier to energy consumption efficiency. A lack of motivation (involving habits and self-conscious intentions or beliefs) was the least decisive factor in the adoption of efficient energy consumption behaviors.Discussion: We therefore recommend the following interventions: 1) training and enablement addressing residents’ physical capability (primarily the replacement of high consumption equipment); 2) training, restriction, environmental restructuring, and enablement would increase residents’ physical opportunity (arising from poor home insulation and citizens’ lack of financial resources to invest in energy solutions); and 3) education, training, and enablement to change psychological capability (regarding insufficient or confusing energy use information).
Fire, volcanism and climate change: the main factors controlling mercury (Hg) accumulation rates in Tropical Lake Lantoa, Sulawesi, Indonesia (∼16,500–540 cal yr BP)
Authors: Nalbant Jalene , Schneider Larissa , Hamilton Rebecca , Connor Simon , Biester Harald , Stuart-Williams Hilary , Bergal-Kuvikas Olga , Jacobsen Geraldine , Stevenson Janelle 
Published: October 2023

The effects of climate change on long-term mercury (Hg) cycling are still not well understood, as climate changes are usually gradual and can only be assessed using high-resolution archives. Our study site (a small, lowland tectonic lake in Sulawesi, Indonesia) provides a unique opportunity to further understanding of Hg cycling in the Southeast Asian (SEA) tropics during the transition from the Pleistocene to the Holocene, a period of significant climate variability. We present a high-resolution record of Late Glacial and Holocene Hg deposition within the sediments of tropical Lake Lantoa, Sulawesi. Using a multi-proxy framework (including pollen, charcoal, carbon:nitrogen ratio and high-resolution geochemistry records) we investigate the response of Hg accumulation rates (HgAR) in sediments to shifts in climate between ∼16,488 and 538 cal BP. This period encompasses the Bølling-Allerød (BA) warming, Younger Dryas (YD) cooling and Holocene warming events, providing new insights into the effects of global climatic transitions on HgAR in SEA sediments. The Pleistocene Termination had the highest HgAR and substantial variability (µ = 11.32, 5.38–33.91 μg m−2 yr−1), when drier conditions and high charcoal accumulation rates suggest that fire activity was the main source of Hg to the lake. The Holocene Transition was marked by a decrease in HgAR (µ = 8, 3.50–18.84 μg m−2 yr−1) as humid conditions precluded forest burning, followed by high HgAR (µ = 11.35, 3.30–158.32 μg m−2 yr−1) in the Early Holocene. Mercury accumulation rate in the Late Holocene (µ = 3.80, 1,67–43.65 μg m−2 yr−1) was the lowest in the Lake Lantoa record, marked by the lowest fire events and a stable catchment. An increase in carbon:nitrogen ratios during the Late Holocene, coupled with a decrease in HgAR, suggests that the establishment of lowland forest resulted in suppressed Hg erosion/leaching. Our results demonstrate that forest fires, vegetation change and volcanism are important drivers of Hg inputs to Lake Lantoa, a relationship which is strongly mediated by climate and lake-catchment dynamics.
Transcriptome analysis of divergent residual feed intake phenotypes in the M. longissimus thoracis et lumborum of Wannan Yellow rabbits
Authors: Huang Dongwei , Wang Yuanlang , Qi Pingping , Ding Haisheng , Zhao Huiling 
Published: October 2023

Introduction: Feed efficiency is an important economic trait in rabbit meat production. The identification of molecular mechanisms and candidate genes for feed efficiency may improve the economic and environmental benefits of the rabbit meat industry. As an alternative to the conventional feed conversion ratio, residual feed intake (RFI) can be used as an accurate indicator of feed efficiency.Methods: RNA sequencing was used to identify the differentially expressed genes (DEGs) in the M. longissimus thoracis et lumborum of eight Wannan Yellow rabbits with excessively high or low RFIs (HRFI or LRFI, respectively). Thereafter, Gene Ontology (GO) analysis, enrichment using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, gene set enrichment analysis (GSEA), and protein–protein interaction (PPI) network analysis was conducted.Results: In total, 445 DEGs were identified in the M. longissimus thoracis et lumborum of rabbits with high and low RFIs. The significantly enriched GO terms identified in these two groups were primarily involved in energy and mitochondrial metabolism and oxidation–reduction processes. KEGG analysis identified 11 significantly enriched pathways, including oxidative phosphorylation, PI3K-Akt signaling, and extracellular matrix-receptor interaction pathways. According to GSEA, the expressions of genes and pathways related to mitochondrial function were upregulated in HRFI rabbits, whereas genes with upregulated expressions in LRFI rabbits were related to immune response and energy metabolism. Additionally, PPI network analysis revealed five potential candidate genetic markers.Conclusion: Comparative analysis of the M. longissimus thoracis et lumborum transcriptomes in HRFI and LRFI rabbits revealed FOS, MYC, PRKACB, ITGA2, and FN1 as potential candidate genes that affect feed efficiency in rabbits. In addition, key signaling pathways involved in oxidative phosphorylation and PI3K-Akt and ECM-receptor interaction signaling impact rabbit feed efficiency. These findings will aid in breeding programs to improve feed efficiency and optimize RFI selection of rabbits for meat production.
Recurrence of common bile duct stones after choledocholithotomy in elderly patients: risk factor analysis and clinical prediction model development
Authors: Wang Han , He Yu-qi , Dong Shi-yang , Zhong Wan , Tao Ping , Yang Shi-yong , Liu Zi-jun 
Published: October 2023

BackgroundThe reasons for the recurrence of common bile duct stones (CBDS) in elderly patients after choledocholithotomy are still unclear. This study aims to establish a prediction model for CBDS recurrence by identifying risk factors.MethodsWe conducted a retrospective analysis of 1804 elderly patients aged 65 years and above who were diagnosed to have CBDS and were admitted to Nanjing First Hospital between January 1, 2010, and January 1, 2021. According to inclusion and exclusion criteria, 706 patients were selected for the final analysis. The patients were assigned to two groups according to the presence or absence of CBDS recurrence, and their clinical data were then statistically analyzed. Subsequently, a prediction model and nomogram were developed, evaluating effectiveness using the concordance index (C-index).ResultsOf the 706 elderly patients, 62 patients experienced CBDS recurrence after surgery, resulting in a recurrence rate of 8.8%. The multivariate Cox analysis showed that prior history of cholecystectomy (hazard ratio [HR] = 1.931, 95% confidence interval [CI]: 1.051–3.547, p = 0.034), white blood cell (WBC) count ≥11.0 × 109/L (HR = 2.923, 95% CI: 1.723–4.957, p &lt; 0.001), preoperative total bilirubin (TBIL) level ≥ 36.5 mmol/L (HR = 2.172, 95% CI: 1.296–3.639, p = 0.003), number of stones ≥2 (HR = 2.093, 95% CI: 1.592–5.294, p = 0.001), maximum stone diameter ≥ 0.85 cm (HR = 1.940, 95% CI: 1.090–3.452, p = 0.024), and T-tube drainage (HR = 2.718, 95% CI: 1.230–6.010, p = 0.013) were independent risk factors of CBDS recurrence in elderly patients after choledocholithotomy. A postoperative CBDS recurrence prediction model was constructed with a C-index value of 0.758 (95% CI: 0.698–0.818) and internal validation value of 0.758 (95% CI: 0.641–0.875).ConclusionA history of cholecystectomy, WBC count ≥11.0 × 109/L, preoperative TBIL level ≥ 36.5 mmol/L, number of stones ≥2, maximum stone diameter ≥ 0.85 cm, and T-tube drainage are the independent risk factors of CBDS recurrence after choledocholithotomy in elderly patients. Our developed prediction model for CBDS recurrence has good predictive ability and can help predict the prognosis of patients with CBDS.
Mental health first aid training among healthcare French students: a qualitative study
Authors: Sibeoni Jordan , Ellul Pierre , Bubola Théo , Debiche Yanis , Piot Marie-Aude 
Published: October 2023

BackgroundHealthcare students are a population more at risk for mental health issues, especially anxiety, depression, and suicidal thoughts. The health faculty of Université Paris Cité in France, Paris has implemented a Mental Health First Aid (MHFA) course aiming to improve students’ mental health literacy, self-care and peer-support and to decrease stigma about mental illness. We conducted a qualitative study exploring the lived experience of this MHFA training course among healthcare students so to better assess its implementation within this specific context and population.MethodsThis qualitative study used the five-stage inductive process to analyze the structure of lived experience (IPSE) approach. All the healthcare students that had completed the 2-day MHFA training were approached to participate. Data was collected through individual semi-structured interviews and inclusion continued until data saturation was reached. Data analysis was based on an inductive, descriptive, and structuring procedure to determine the structure of lived experience characterized by the central axes of experience.ResultsTwenty students were included. Data analysis produced a common structure of lived experience based on three central axes of experience, (1) a personal experience, (2) a student experience and (3) a professional experience. The participants all experienced this course intertwined within these 3 axes. Their motivation to take the course was personal -being of feeling concerned by the topic-, was study-oriented – to learn and revise psychiatry- and was professional – so to develop both practical and soft skills. In their personal experience, participants reported a transformative experience and some interventions with friends and family, while both in their student and professional experience, they felt frustrated with both the content and the form of the course.ConclusionThe results reported similar outcomes reported in the literature about skills, knowledge, and awareness; but mostly produce original avenues about how to better adapt such course to this specific population so to better address students’ expectations and mental health issues. This MHFA course -with an adapted content addressing eating disorders, self-mutilations and sexual and gender-based violence – could be part of the early curriculum of healthcare students. The latter could then benefit from a level 2/advanced MHFA course years later specifically tailored for healthcare professionals.
In focus: perplexing increase of urinary stone disease in children, adolescent and young adult women and its economic impact
Authors: Filler Guido , Dave Sumit , Ritter Victor , Ross Sherry , Viprakasit Davis , Hatch Joseph E. , Bjazevic Jennifer , Burton Jeremy , Gilleskie Donna , Gilliland Jason , Lin Feng-Chang , Jain Nina , McClure J. Andrew , Razvi Hassan , Bhayana Vipin , Wang Peter , Coulson Sherry , Sultan Nabil , Denstedt John , Fearrington Loretta , Diaz-Gonzalez de Ferris Maria E. 
Published: October 2023

BackgroundUrinary stone disease (USD) historically has affected older men, but studies suggest recent increases in women, leading to a near identical sex incidence ratio. USD incidence has doubled every 10 years, with disproportionate increases amongst children, adolescent, and young adult (AYA) women. USD stone composition in women is frequently apatite (calcium phosphate), which forms in a higher urine pH, low urinary citrate, and an abundance of urinary uric acid, while men produce more calcium oxalate stones. The reasons for this epidemiological trend are unknown.MethodsThis perspective presents the extent of USD with data from a Canadian Province and a North American institution, explanations for these findings and offers potential solutions to decrease this trend. We describe the economic impact of USD.FindingsThere was a significant increase of 46% in overall surgical interventions for USD in Ontario. The incidence rose from 47.0/100,000 in 2002 to 68.7/100,000 population in 2016. In a single United States institution, the overall USD annual unique patient count rose from 10,612 to 17,706 from 2015 to 2019, and the proportion of women with USD was much higher than expected. In the 10–17-year-old patients, 50.1% were girls; with 57.5% in the 18–34 age group and 53.6% in the 35–44 age group. The roles of obesity, diet, hormones, environmental factors, infections, and antibiotics, as well as the economic impact, are discussed.InterpretationWe confirm the significant increase in USD among women. We offer potential explanations for this sex disparity, including microbiological and pathophysiological aspects. We also outline innovative solutions – that may require steps beyond typical preventive and treatment recommendations.
Alginate-based hydrogel platform embedding silver nanoparticles and cisplatin: characterization of the synergistic effect on a breast cancer cell line
Authors: Maher Shaimaa , Kalil Haitham , Liu Guiming , Sossey-Alaoui Khalid , Bayachou Mekki 
Published: October 2023

Introduction: Breast cancer is a significant cause of mortality in women globally, and current treatment approaches face challenges due to side effects and drug resistance. Nanotechnology offers promising solutions by enabling targeted drug delivery and minimizing toxicity to normal tissues.Methods: In this study, we developed a composite platform called (Alg-AgNPs-CisPt), consisting of silver nanoparticles coated with an alginate hydrogel embedding cisplatin. We examined the effectiveness of this nanocomplex in induce synergistic cytotoxic effects on breast cancer cells.Results and Discussion: Characterization using various analytical techniques confirmed the composition of the nanocomplex and the distribution of its components. Cytotoxicity assays and apoptosis analysis demonstrated that the nanocomplex exhibited greater efficacy against breast cancer cells compared to AgNPs or cisplatin as standalone treatments. Moreover, the nanocomplex was found to enhance intracellular reactive oxygen species levels, further validating its efficacy. The synergistic action of the nanocomplex constituents offers potential advantages in reducing side effects associated with higher doses of cisplatin as a standalone treatment. Overall, this study highlights the potential of the (Alg-AgNPs-CisPt) nanocomplex as a promising platform embedding components with synergistic action against breast cancer cells.