bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of an App-Based Physical Activity Program on Colorectal Cancer Patients Undergoing Chemotherapy
Authors: Hwang Yun Jeong , Kim Ik Yong , Hur Hea Kung , Lee Ji Yong , Park SoMi 
Published: January 2024

Background
Colorectal cancer is one of the most common malignancies worldwide. Oxaliplatin, which is used as adjuvant chemotherapy, affects quality of life by causing oxaliplatin-induced peripheral neuropathy in colorectal cancer patients.

Objectives
This study examined the effects of an application (app)–based physical activity program for alleviating peripheral neuropathy symptoms in colorectal cancer patients undergoing chemotherapy.

Methods
This was a randomized controlled study that included 34 patients undergoing chemotherapy after being diagnosed with colorectal cancer. Outcomes were compared between patients who participated in a 6-week app-based physical activity program (experimental group; n = 17) and who received standard booklet education (control group; n = 17). Data were collected using questionnaires, and exercise time was recorded to evaluate intervention adherence.

Results
Significant differences were observed between the groups in peripheral neuropathy symptoms (F = 8.93, P = .002), interference with activities (Z = −2.55, P = .011), and quality of life (F = 7.65, P = .003). The experimental group showed significantly higher average exercise times at 1 to 4 weeks (Z = −2.10, P = .026), 5 to 6 weeks (Z = −4.02, P < .001), and 1 to 6 weeks (Z = −3.40, P = .001) than the control group.

Conclusions
The app-based physical activity program had a positive effect on participants’ exercise adherence and reduced peripheral neuropathy symptoms. Thus, we propose the adoption of a mobile health app that can be used at any time or place as an intervention for preventing or alleviating adverse effects during the treatment of cancer patients.

Implications for Practice
An app-based physical activity program using the mobile health app can be used as a nursing intervention to manage symptoms and increase the health behavior adherence in cancer patients.
Alteration in Resting-State Brain Activity in Stroke Survivors After Repetitive Finger Stimulation
Authors: He Dorothy , Sikora William A. , James Shirley A. , Williamson Jordan N. , Lepak Louis V. , Cheema Carolyn F. , Sidorov Evgeny , Li Sheng , Yang Yuan 
Published: January 2024

Objective
This quasi-experimental study examined the effect of repetitive finger stimulation on brain activation in eight stroke and seven control subjects, measured by quantitative electroencephalogram.

Methods
We applied 5 mins of 2-Hz repetitive bilateral index finger transcutaneous electrical nerve stimulation and compared differences pre– and post–transcutaneous electrical nerve stimulation using quantitative electroencephalogram metrics delta/alpha ratio and delta-theta/alpha-beta ratio.

Results
Between-group differences before and after stimulation were significantly different in the delta/alpha ratio (z = −2.88, P = 0.0040) and the delta-theta/alpha-beta ratio variables (z = −3.90 with P < 0.0001). Significant decrease in the delta/alpha ratio and delta-theta/alpha-beta ratio variables after the transcutaneous electrical nerve stimulation was detected only in the stroke group (delta/alpha ratio diff = 3.87, P = 0.0211) (delta-theta/alpha-beta ratio diff = 1.19, P = 0.0074).

Conclusions
The decrease in quantitative electroencephalogram metrics in the stroke group may indicate improved brain activity after transcutaneous electrical nerve stimulation. This finding may pave the way for a future novel therapy based on transcutaneous electrical nerve stimulation and quantitative electroencephalogram measures to improve brain recovery after stroke.
Nonpharmacological Therapies for the Management of the Cognitive Dysfunctions in Poststroke Patients
Authors: Liu Yali , Liang Yue , Gao Wei , Dai Tianjiao , Wang Lezheng , Ji Xiaoqi , Chen Meng , Zhou Supo , Zou Ying , Sun Xiaofei , Wu Bao 
Published: January 2024

Abstract

Objective
The aim of the study is to investigate the comparative effects of nonpharmacological therapies for managing global, attention, memory, and execution cognitive functions in stroke patients.

Design
We searched PubMed, Embase, CINAHL, Cochrane Library, Web of Science, PEDro, and Google Scholar for randomized controlled trials that evaluated the effects of nonpharmacological therapies for treating stroke cognitive dysfunctions. We performed a network meta-analysis to estimate the mean treatment effect of 95% credible interval.

Results
Seventy-three randomized controlled trials were included in the network meta-analysis for evidence syntheses. All therapies had significant effects than control on global cognition in stroke patients. Combined therapy was superior to other therapies for global cognition of all patients (vs. cognitive task therapy: 0.71, 95% credible interval = 0.14 to 1.29; vs. exercise: 0.88, 95% credible interval = 0.31 to 1.45, vs. physical modality therapy: 0.77, 95% credible interval = 0.16 to 1.40). Different therapies have effects on specific cognitive domains in stroke patients.

Conclusions
Our findings suggest that nonpharmacological therapies are effective in improving global cognitive function in stroke patients, with cognitive task therapy, exercise therapy, physical modality therapy, and combined therapy being viable options (most optimal approach: combined therapy). Precise selection of therapies based on the time since stroke onset and specific cognitive domains can further enhance treatment outcomes.
Elective Cholecystectomy After Endoscopic Gallbladder Stenting for Acute Cholecystitis: A Propensity Score Matching Analysis
Authors: Kobayashi Shinjiro , Nakahara Kazunari , Umezawa Saori , Ida Keisuke , Tsuchihashi Atsuhito , Koizumi Satoshi , Sato Junya , Tateishi Keisuke , Otsubo Takehito 
Published: January 2024

Objective:
To investigate the influence of endoscopic gallbladder stenting (EGBS) on subsequent cholecystectomy. We retrospectively compared the surgical outcomes of EGBS, followed by elective cholecystectomy with those of immediate cholecystectomy (IC).

Patients and Methods:
A total of 503 patients were included in this study. Patients who underwent EGBS as initial treatment for acute cholecystitis, followed by elective cholecystectomy, were included in the EGBS group and patients who underwent IC during hospitalization were included in the IC group. Propensity score matching analysis was used to compare the surgical outcomes. In addition, the factors that increased the amount of bleeding were examined by multivariate analysis after matching.

Results:
Fifty-seven matched pairs were obtained after propensity matching the EGBS group and the IC group. The rate of laparoscopic cholecystectomy in the EGBS versus IC groups was 91.2% versus 49.1% (P < 0.001). The amount of bleeding was 5 mL in the EGBS versus 188 mL in the IC group (P < 0.001). In the EGBS and IC groups, multivariate analysis of factors associated with more blood loss revealed IC (odds ratio: 4.76, 95% CI: 1.25-20.76, P = 0.022) as an independent risk factor.

Conclusion:
EGBS as the initial treatment for acute cholecystitis and subsequent elective cholecystectomy after the inflammation has disappeared can be performed in minimally invasive procedures and safely.
PD-1 Targeted Antibody Discovery Using AI Protein Diffusion
Authors: Ford Colby T. 
Published: January 2024

AbstractThe programmed cell death protein 1 (PD-1, CD279) is an important therapeutic target in many oncological diseases. This checkpoint protein inhibits T lymphocytes from attacking other cells in the body and thus blocking it improves the clearance of tumor cells by the immune system. While there are already multiple FDA-approved anti-PD-1 antibodies, including nivolumab (Opdivo® from Bristol-Myers Squibb) and pembrolizumab (Keytruda® from Merck), there are ongoing efforts to discover new and improved checkpoint inhibitor therapeutics. In this study, we present multiple anti-PD-1 antibody fragments that were derived computationally using protein diffusion and evaluated through our scalable,in silicopipeline. Here we present nine synthetic Fv structures that are suitable for further empirical testing of their anti-PD-1 activity due to desirable predicted binding performance.
Non-invasive multi-cancer detection using DNA hypomethylation of LINE-1 retrotransposons
Authors: Michel Marc , Heidary Maryam , Mechri Anissa , Da Silva Kévin , Gorse Marine , Dixon Victoria , von Grafenstein Klaus , Bianchi Charline , Hego Caroline , Rampanou Aurore , Lamy Constance , Kamal Maud , Le Tourneau Christophe , Séné Mathieu , Bièche Ivan , Reyes Cecile , Gentien David , Stern Marc-Henri , Lantz Olivier , Cabel Luc , Pierga Jean-Yves , Bidard François-Clément , Azencott Chloé-Agathe , Proudhon Charlotte 
Published: January 2024

AbstractPurposeThe detection of circulating tumor DNA, which allows non-invasive tumor molecular profiling and disease follow-up, promises optimal and individualized management of patients with cancer. However, detecting small fractions of tumor DNA released when the tumor burden is reduced remains a challenge.Experimental DesignWe implemented a new highly sensitive strategy to detect base-pair resolution methylation patterns from plasma DNA and assessed the potential of hypomethylation of LINE-1 retrotransposons as a non-invasive multi-cancer detection biomarker. The DIAMOND (Detection of Long Interspersed Nuclear Element Altered Methylation ON plasma DNA) method targets 30-40,000 young L1 scattered throughout the genome, covering about 100,000 CpG sites and is based on a reference-free analysis pipeline.ResultsResulting machine learning-based classifiers showed powerful correct classification rates discriminating healthy and tumor plasmas from 6 types of cancers (colorectal, breast, lung, ovarian, gastric cancers and uveal melanoma including localized stages) in two independent cohorts (AUC = 88% to 100%, N = 747). DIAMOND can also be used to perform copy number alterations (CNA) analysis which improves cancer detection.ConclusionsThis should lead to the development of more efficient non-invasive diagnostic tests adapted to all cancer patients, based on the universality of these factors.Statement of significanceThe DIAMOND assay is a new highly sensitive strategy to detect base-pair resolution methylation patterns of LINE-1 retrotransposons (L1) from plasma DNA. It targets 30-40,000 young L1 scattered throughout the genome, covering about 100,000 CpG sites and is based on a reference-free analysis pipeline. This provided high coverage data using affordable sequencing depth, which is instrumental to achieve high sensitivity and work with minute amounts of cell-free DNA. Resulting machine learning-based classifiers showed powerful discrimination between healthy and tumor plasmas from 6 types of cancers (colorectal, breast, lung, ovarian, gastric cancers and uveal melanoma including localized stages) in two independent cohorts (AUC = 88% to 100%, N = 747). DIAMOND data can also be used to perform copy number alterations (CNA) analysis which improves cancer detection.
Synchronization of Human Circadian Genes Betweenin vivoand Cultured Blood Samples
Authors: Pritt Jacob , Counts Julie , Campbell Milton , Reed David , Kraus William 
Published: January 2024

AbstractTranscriptomic studies of human circadian rhythm are limited in efficacy by the invasiveness of sampling. Studies of circadian dynamics should ideally use multiple blood samples drawn at regular intervals over the course of a day. This is difficult to achieve, particularly at night, without disrupting the very circadian rhythm that is being studied. We propose a method by which blood is drawn at a single initial timepoint, then cultured and repeatedly sampled over the course of a day. This method is minimally invasive to the subject. Our results demonstrate that the expression levels of circadian genes are more closely correlated between the cultured (ex vivo) cells and live (in vivo) samples than non-circadian genes, suggesting that this method can be used for effective circadian analysis.
First-night effect reduces the beneficial effects of sleep on visual plasticity and modifies the underlying neurochemical processes
Authors: Tamaki Masako , Yamada Takashi , Barnes-Diana Tyler , Wang Zhiyan , Watanabe Takeo , Sasaki Yuka 
Published: January 2024

SummaryIndividuals experience difficulty falling asleep in a new environment, termed the first night effect (FNE). However, the impact of the FNE on sleep-induced brain plasticity remains unclear. Here, using a within-subject design, we found that the FNE significantly reduces visual plasticity during sleep in young adults. Sleep-onset latency (SOL), an indicator of the FNE, was significantly longer during the first sleep session than the second session, confirming the FNE. We assessed performance gains in visual perceptual learning after sleep and increases in the excitatory-to-inhibitory neurotransmitter (E/I) ratio in early visual areas during sleep using magnetic resonance spectroscopy and polysomnography. These parameters were significantly smaller in sleep with the FNE than in sleep without the FNE; however, these parameters were not correlated with SOL. These results suggest that while the neural mechanisms of the FNE and brain plasticity are independent, sleep disturbances temporarily block the neurochemical process fundamental for brain plasticity.
Alpha phase-coding supports feature binding during working memory maintenance
Authors: Pagnotta Mattia F. , Santo-Angles Aniol , Temudo Ainsley , Barbosa Joao , Compte Albert , D’Esposito Mark , Sreenivasan Kartik K. 
Published: January 2024

AbstractThe ability to successfully retain and manipulate information in working memory (WM) requires that objects’ individual features are bound into cohesive representations; yet, the mechanisms supporting feature binding remain unclear. Binding (or swap) errors, where memorized features are erroneously associated with the wrong object, can provide a window into the intrinsic limits in capacity of WM that represent a key bottleneck in our cognitive ability. We tested the hypothesis that binding in WM is accomplished via neural phase synchrony and that swap errors result from perturbations in this synchrony. Using magnetoencephalography data collected from human subjects in a task designed to induce swap errors, we showed that swaps are characterized by reduced phase-locked oscillatory activity during memory retention, as predicted by an attractor model of spiking neural networks. Further, we found that this reduction arises from increased phase-coding variability in the alpha-band over a distributed network of sensorimotor areas. Our findings demonstrate that feature binding in WM is accomplished through phase-coding dynamics that emerge from the competition between different memories.
The choroid plexus maintains ventricle volume and adult subventricular zone neuroblast pool, which facilitates post-stroke neurogenesis
Authors: Taranov Aleksandr , Bedolla Alicia , Iwasawa Eri , Brown Farrah N. , Baumgartner Sarah , Fugate Elizabeth M. , Levoy Joel , Crone Steven A. , Goto June , Luo Yu 
Published: January 2024

AbstractThe brain’s neuroreparative capacity after injuries such as ischemic stroke is contained in the brain’s neurogenic niches, primarily the subventricular zone (SVZ), which lies in close contact with the cerebrospinal fluid (CSF) produced by the choroid plexus (ChP). Despite the wide range of their proposed functions, the ChP/CSF remain among the most understudied compartments of the central nervous system (CNS). Here we report a mouse genetic tool (the ROSA26iDTR mouse line) for non-invasive, specific, and temporally controllable ablation of CSF-producing ChP epithelial cells to assess the roles of the ChP and CSF in brain homeostasis and injury. Using this model, we demonstrate that ChP ablation causes rapid and permanent CSF volume loss accompanied by disruption of ependymal cilia bundles. Surprisingly, ChP ablation did not result in overt neurological deficits at one-month post-ablation. However, we observed a pronounced decrease in the pool of SVZ neuroblasts following ChP ablation, which occurs due to their enhanced migration into the olfactory bulb. In the MCAo model of ischemic stroke, neuroblast migration into the lesion site was also reduced in the CSF-depleted mice. Thus, our study establishes an important and novel role of ChP/CSF in regulating the regenerative capacity of the adult brain under normal conditions and after ischemic stroke.
CD8+T cell response promotes viral clearance and reduces chances of severe testicular damage in mouse models of long-term Zika virus infection of the testes
Authors: Campos Rafael K. , Liang Yuejin , Azar Sasha R. , Ly Judy , Camargos Vidyleison Neves , Hager-Soto E. Eldridge , Eyzaguirre Eduardo , Sun Jiaren , Rossi Shannan L. 
Published: January 2024

AbstractZika virus (ZIKV) causes human testicular inflammation and alterations in sperm parameters and causes testicular damage in mouse models. The involvement of individual immune cells in testicular damage is not fully understood. We detected virus in the testes of the interferon (IFN) α/β receptor-/-A129 mice three weeks post-infection and found elevated chemokines in the testes, suggesting chronic inflammation and long-term infection play a role in testicular damage. In the testes, myeloid cells and CD4+T cells were absent at 7 dpi but were present at 23 days post-infection (dpi), and CD8+T cell infiltration started at 7 dpi. CD8-/-mice with an antibody-depleted IFN response had a significant reduction in spermatogenesis, indicating that CD8+T cells are essential to prevent testicular damage during long-term ZIKV infections. Our findings on the dynamics of testicular immune cells and importance of CD8+T cells functions as a framework to understand mechanisms underlying observed inflammation and sperm alterations in humans.
More social species live longer, have higher generation times, and longer reproductive windows
Authors: Salguero-Gómez Roberto 
Published: January 2024

AbstractThe role of sociality in the demography of animals has become an intense focus of research in recent decades. However, efforts to understand the sociality-demography nexus have focused on single species or isolated taxonomic groups. Consequently, we lack generality regarding how sociality associates with demographic traits within the Animal Kingdom. Here, I propose a continuum of sociality, from solitary to tightly social, and test whether this continuum correlates with the key demographic properties of 152 species, from jellyfish to humans. After correction for body mass and phylogenetic relationships, I show that the sociality continuum is associated with key life history traits: more social species live longer, postpone maturity, have greater generation time, and greater probability of achieving reproduction than solitary, gregarious, communal, or colonial species. Contrary to the social buffering hypothesis, sociality does not result in more buffered populations. While more social species have a lower ability to benefit from disturbances, they display greater resistance than more solitary species. Finally, I also show that sociality does not shape reproductive or actuarial senescence rates. This cross-taxonomic examination of sociality across the demography of 13 taxonomic classes highlights keyways in which individual interactions shape most aspects of animal demography.
MTMR regulates KRAS function by controlling plasma membrane levels of phospholipids
Authors: Lange Taylor E. , Naji Ali , van der Hoeven Ransome , Liang Hong , Zhou Yong , Hammond Gerald R.V. , Hancock John F. , Cho Kwang-jin 
Published: January 2024

AbstractKRAS, a small GTPase involved in cell proliferation and differentiation, frequently gains activating mutations in human cancers. For KRAS to function, it must bind the plasma membrane (PM) via interactions between its membrane anchor and phosphatidylserine (PtdSer). Therefore, depleting PM PtdSer abrogates KRAS PM binding and activity. From a genome-wide siRNA screen to identify genes regulating KRAS PM localization, we identified a set of phosphatidylinositol (PI) 3-phosphatases: myotubularin-related proteins (MTMR) 2, 3, 4, and 7. Here, we show that silencingMTMR 2/3/4/7disrupts KRAS PM interactions by reducing PM PI 4-phosphate (PI4P) levels, thereby disrupting the localization and operation of ORP5, a lipid transfer protein maintaining PM PtdSer enrichment. Concomitantly, silencingMTMR 2/3/4/7elevates PM PI3P levels while reducing PM and total PtdSer levels. We also observed MTMR 2/3/4/7 expression is interdependent. We propose that the PI 3-phosphatase activity of MTMR is required for generating PM PI, necessary for PM PI4P synthesis, promoting the PM localization of PtdSer and KRAS.eTOC summaryWe discovered that silencing the phosphatidylinositol (PI) 3-phosphatase,MTMR, disrupts the PM localization of PtdSer and KRAS. We propose a model, whereMTMRloss depletes PM PI needed for PM PI4P synthesis, an essential phospholipid for PM PtdSer enrichment, thereby impairing KRAS PM localization.
Specificities and commonalities of the Planctomycetes plasmidome
Authors: Coronel María del Mar Quiñonero , Devos Damien Paul , Garcillán-Barcia M. Pilar 
Published: January 2024

ABSTRACTAlthough plasmids play a crucial role in antibiotic resistance and contemporary biotechnology, our comprehension of their natural ecological dynamics remains restricted to a few bacterial groups. Planctomycetes is a bacterial phylum with unusual molecular and cellular biology for which little is known about its plasmidome. This study provides the first comprehensive description of the diversity of the endogenous plasmids found in Planctomycetes, which may be used as starting points to generate different genetic tools for research on this phylum. Plasmids from Planctomycetes encode a wide variety of biological functions, although for a large portion of the encoded genes a function cannot be assigned, which is usual in Planctomycetes. They seemed to have largely coevolved with the genome of their hosts, with which they share many homologs. We also detected recent transfer events of insertion sequences between co-habiting chromosomes and plasmids. 60% of the plasmid genes are distantly related to chromosomally-encoded genes and 40% have homologs in plasmids from other bacterial groups, while 36% of the proteins composing the planctomycetal plasmidome are exclusive. Most planctomycetal plasmids encode a replication initiation protein of the RPA family in the proximity of a putative iteron-containing replication origin, as well as active type I partition systems. One conjugative and three mobilizable plasmids were identified, suggesting horizontal gene transfer via conjugation in this phylum.