bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous PCR detection of Paenibacillus larvae targeting insertion sequence IS256 and Melissococcus plutonius targeting pMP1 plasmid from hive specimens
Authors: Vlkova Katerina , Erban Tomas , Kamler Martin , Titera Dalibor , Bitar Ibrahim , Hrabak Jaroslav 
Published: January 2024

AbstractPaenibacillus larvae and Melissococcus plutonius represent the most threatening bacterial diseases of honeybee (Apis mellifera)—American and European foulbrood, respectively. For efficient control of those diseases, rapid and accurate detection of the pathogens is crucial. Therefore, we developed a novel multiplex PCR method simultaneously detecting both pathogens. To design and optimize multiplex PCR reaction, four strains of P. larvae representing four ERIC genotypes I–IV (strain DSM 7030—ERIC I, DSM 25430—ERIC II, LMG 16252—ERIC III, DSM 3615—ERIC IV) were selected. Those strains were fully sequenced using long-read sequencing (Sequel I, Pacific Biosciences). For P. larvae, the multicopy insertion sequence IS256 identified in all genotypes of P. larvae was selected to provide high sensitivity. M. plutonius was detected by plasmid pMP1 sequence and the virulence verified by following detection of ETX/MTX2 toxin responsible for pore formation in the cell membrane. As an internal control, a gene encoding for major royal jelly protein 1 specific for honeybees was selected. The method was validated on 36 clinical specimens collected from the colonies suffering from American and European foulbrood in the Czech Republic. Based on the results, sensitivity of PCR was calculated to 93.75% and specificity to 100% for P. larvae diagnosed from hive debris and 100% sensitivity and specificity for honeybee workers and larval scales as well as for diseased brood infected by M. plutonius.
A novel approach of kinship determination based on the physical length of genetically shared regions of chromosomes
Authors: Cho Sohee , Shin Eunsoon , Park Yoon Gi , Choi Seung Ho , Choe Eun Kyung , Bae Jung Ho , Lee Jong-Eun , Lee Soong Deok 
Published: January 2024

Abstract
Background
Determination of genetic relatedness between individuals plays a crucial role in resolving numerous civil cases involving familial relationships and in forensic investigation concerning missing persons. Short tandem repeats (STRs), known for their high degree of DNA polymorphism, have traditionally been the primary choice of DNA markers in genetic testing, but their application for kinships testing is limited to cases involving close kinship. SNPs have emerged as promising supplementary markers for kinship determination. Nevertheless, the challenging remains in discriminating between third-degree or more distant relatives, such as first cousins, using SNPs.

Objective
To investigate a kinship analysis method for distant degree of familial relationships using high-density SNP data.

Methods
A high-density SNP data from 337 individuals of Korean families using Affymetrix Axiom KORV1.0-96 Array was obtained for this study. SNPs were aligned by chromosomal positions, and identity-by-state (IBS) was determined, and then shared regions as consecutive SNPs with IBS of 1 or 2 were investigated. The physical lengths of these IBS segments were measured and summed them to create an Index, as a measure of kinship.

Results
The kinship was determined by the physical length of shared chromosomal regions that are distinguished by each kinship. Using this method, the relationship was able be distinguished up to the fourth degree of kinship, and non-relatives were clearly distinguished from true relatives. We also found a potential for this approach to be used universally, regardless of microarray platforms for SNP genotyping and populations.

Conclusion
This method has a potential to determine the different degree of kinship between individuals and to distinguish non-relatives from true relatives, which can be of great help for practical applications in kinship determination.
Pathogenicity of Puccinia porri on Allium in South Africa
Authors: Boshoff Willem H. P. , Visser Botma , Bender Cornel M. , Pretorius Zacharias A. 
Published: January 2024

AbstractAllium crops are commonly grown in South Africa and harvested as either fresh produce for the domestic and export markets or as seed. Apart from occasional outbreaks on garlic, rust is problematic as a cosmetic disease with unappealing uredinia regularly observed on freshly packed produce of bunching onion and leek in supermarkets. Spore morphology and phylogenetic analysis of five rust samples collected from A. fistulosum (bunching onion) confirmed the causal organism as Puccinia porri. Garlic and bunching onion varieties were mostly susceptible to P. porri, whereas leek varieties were either susceptible or segregating in their response, with bulb onions being resistant. Microscopy of early infection structures showed appressorium formation, stomatal penetration, and a substomatal structure which differentiated into infection hyphae and haustorium mother cells. At microscopy level differences in host response became visible from 48 h post-inoculation onwards with prehaustorial and early hypersensitivity observed as resistance mechanisms in onions.
Chemical characteristics and microbiological loads of harvested rainwater run-off from roof tops in South Eastern Nigeria
Authors: Nwogu F. U. , Ubuoh E. A. , Kanu S. C. 
Published: January 2024

AbstractThe study evaluated rainwater quality harvested from roof top catchments viz Asbestos, Aluminum, Corrugated and Harvey roof tops in southeastern, Nigeria. Chemical and microbiological species viz pH, Total Dissolved Solids(TDS), Electrical conductivity (EC), Nitrate(NO3−), Sulphate (SO42−), Chloride(Cl−), Total bacteria load(TBL), Total Coliform Count (TCC), Salmonella Shigella Agar (SSA), Thiocitrate Bile-salt Sucrose( TCBS), Eosin Methylene Blue (EMB) were investigated from samples of rainwater roof top run off during onset rain (April), peak rain (July) and cessation (October) rain events in 2022. Results of chemical parameters show significant variation p ≥ 0.05 among the catchments with increased mean concentration of pH(6.5), TDS(25.7 mg/l), EC(43.6μS/cm),NO3−(0.72 mg/l) SO42(4.73 mg/l), Cl(8.81) during onset rain more than the peak and cessation rain, with asbestos rainwater having the highest levels but below WHO/FMENV drinking water limit. Positive and negative correlations existed between the chemical parameters in temporal scale and originating from human activities with the temporal variability in decrease order: April ≥ October ≥ July. Microbial parameters in rainwater revealed mean TBL(6.6 × 104 cfu/ml), TCC(2.6 × 103 cfu/ml) and control (5.8 × 104 cfu/ml) with Aluminum top (8.2 × 104 cfu/ml) recording highest in onset rain more than the peak and cessation rain events. All of the microbial parameters were above the WHO standards which indicates a very high public health risk if consumed without treatment. Organisms found in rainwater from roof types are: Enterobacter sp., Staphylococcus aurous, Streptococcus sp., Bacillus Sp. and Escherichia coli. Based on the results, treatment of rainwater, operation, maintenance methods should be upheld for quality assurance of harvested rainwater.
Bacteriostatic and antidiabetic activities of various extracts from the mixture leaves and stems of Suaeda marimita
Authors: Yasin Hussein Alaa , Al Touby Salem Said , Hossain Mohammad Amzad 
Published: January 2024

AbstractHumans were always in need of new medications to fight the many developing diseases that they face. In recent years, the race to develop new medicines has increased caused for new diseases to occur by bacteria and viruses developed causing accumulation, local damage, and inflammation or irritation. Suaeda maritima (S. maritima) is a plant that was used in the past to treat illness and the plant extracts showed several biological activities especially hepatoprotective, antimicrobial, and antidiabetic activities. The present goal of this work is to prepare plant extracts with varied polarities from the mixture of leaves and stems of S. maritima and examine their bacteriostatic and antidiabetic activities by in vitro methods. The crude extract was prepared from the mixture of leaves and stems powder samples by methanol using the maceration method for 36 h. The methanol was removed from the extract by the usual method and then fractionated methanol extract with different polarities solvents. The bacteriostatic and antidiabetic activities of plant extracts were determined by using disc diffusion and enzyme methods. The prepared varied polarities extract at four different concentrations (2000, 1000, 500, and 250 µg/mL) were used to evaluate their bacteriostatic activity against the Gram (+ and −) bacterial strains. Among the six plant extracts, ethyl acetate extract showed the highest bacterial activity, and the lowest activity was in DCM extract. However, at low concentrations, almost all plant extracts of the selected plant didn’t show any bacteriostatic activity. The antidiabetic activity of plant varied extracts was assessed by using an α-glucosidase bioassay and the highest IC50 was obtained in methanol extract and the lowest was in hexane extract from this bioassay. The other plant crude extracts also showed promising activity against the α-glucosidase bioassay. The plant extracts with significant activity from the selected plant could be used as natural medication to treat diabetes.
Every complex Hénon map is exponentially mixing of all orders and satisfies the CLT
Authors: Bianchi Fabrizio , Dinh Tien-Cuong 
Published: January 2024

Abstract
We show that the measure of maximal entropy of every complex Hénon map is exponentially mixing of all orders for Hölder observables. As a consequence, the Central Limit Theorem holds for all Hölder observables.
Modularity of trianguline Galois representations
Authors: Bellovin Rebecca 
Published: January 2024

Abstract
We use the theory of trianguline

$(\varphi ,\Gamma )$

-modules over pseudorigid spaces to prove a modularity lifting theorem for certain Galois representations which are trianguline at p, including those with characteristic p coefficients. The use of pseudorigid spaces lets us construct integral models of the trianguline varieties of [BHS17], [Che13] after bounding the slope, and we carry out a Taylor–Wiles patching argument for families of overconvergent modular forms. This permits us to construct a patched quaternionic eigenvariety and deduce our modularity results.
Commentary on Perspective Article: ‘Institutional Logics: Motivating Action and Overcoming Resistance to Change’ – Heather A. Haveman, David Joseph-Goteiner, and Danyang Li
Authors: Raynard Mia , Greenwood Royston 
Published: January 2024

AbstractHaveman, Joseph-Goteiner, and Li's (2023) perspective article contributes important insights into China's transition away from central planning and redistribution toward greater market coordination of economic exchange. In our commentary on their insightful article, we build on and extend their arguments in three main ways. First, we discuss how future studies might extend the authors’ work by leveraging the ‘messiness’ of institutional change to explore the cross-level dynamics involved in transforming institutional logics. Second, we build on the authors’ call for more historically grounded, contextualized research on institutional logics to argue that the conditions surrounding logic emergence have important implications for inter-logic dynamics and organizational responses. Third, we build on the authors’ suggestions for future research to underscore the broader consequences of institutional logics and their potential to perpetuate or exacerbate social inequalities and other societal challenges.
Dysfunction of circulating endothelial progenitor cells in major depressive disorder
Authors: Liou Ying-Jay , Chen Mu-Hong , Hsu Ju-Wei , Huang Kai-Lin , Huang Po-Hsun , Bai Ya-Mei 
Published: January 2024

AbstractObjectives:Despite mounting evidence demonstrates circulating endothelial progenitor cells (cEPCs) quantitative changes in depression, no study has investigated cEPC functions in major depressive disorder (MDD). We investigated the role of cEPC adhesive and apoptotic functions in MDD.Methods:We recruited 68 patients with MDD and 56 healthy controls (HCs). The depression symptoms, anxiety, psychosomatic symptoms, subjective cognitive dysfunction, quality of life, and functional disability were evaluated using the Hamilton Depression Rating Scale and Montgomery–Åsberg Depression Rating Scale, Hamilton Anxiety Rating Scale, Depression and Somatic Symptoms Scale (DSSS), Perceived Deficits Questionnaire-Depression, 12-Item Short Form Health Survey (SF-12), and Sheehan Disability Scale (SDS), respectively. Working memory and executive function were assessed using a 2-back task and Wisconsin Card Sorting Test (WCST). Inflammatory marker (soluble interleukin-6 receptor, C-reactive protein, and tumor necrosis factor-α receptor-1), cEPC adhesive, and apoptotic levels were measured using in vitro assays.Results:The MDD patients showed significantly lower cEPC adhesive levels than the HCs, and this difference in adhesive function remained statistically significant even after adjusting for inflammatory marker levels. The cEPC adhesion levels were in inverse correlations with commission and omission errors in 2-back task, the percent perseverative response and percent perseverative errors in WCST, and the DSSS and SDS scores, but in positive correlations with SF-12 physical and mental component scores. cEPC apoptotic levels did not differ significantly between the groups.Conclusion:The findings indicate that cEPC adhesive function is diminished in MDD and impacts various aspects of cognitive and psychosocial functions associated with the disorder.
Associations of childhood diet quality scores with arterial stiffness and carotid artery intima-media thickness in adolescence/early adulthood: findings from the ALSPAC cohort
Authors: Buckland Genevieve , Northstone Kate , Emmett Pauline M. , Taylor Caroline M. 
Published: January 2024

AbstractThis study examined the relationship between childhood diet quality and arterial stiffness and thickness during adolescence/early adulthood. Participants were from the Avon Longitudinal Study of Parents and Children (ALSPAC) with dietary data at ages 7, 10 and 13 years and pulse wave velocity (PWV) and carotid intima-media thickness (cIMT) at ages 17 and/or 24 years. Diet quality (DQ) was assessed using five scores: a children’s Mediterranean-style diet (C-rMED) Z-score, a children’s Dietary Inflammatory Z-score (C-DIS), a DASH diet Z-score, a children’s Eatwell Guide (C-EWG) Z-score reflecting UK dietary guidelines and a data-driven obesogenic Z-score. Adjusted regression models examined the associations between DQ scores at 7–13 years and PWV and cIMT at 17 and 24 years. In adjusted models, a high v. low Obesogenic Z-score at 7 and 10 years was associated with higher PWV at 17: β 0.07 (95 % CI 0.01, 0.13) and β 0.10 (95 % CI 0.04, 0.16), respectively. A high v. low C-rMED Z-score at 7 years was associated with lower PWV at 17 (β −0.07; 95 % CI −0.14, −0.01). A high (more anti-inflammatory) vs low C-DIS Z-score at 10 years was associated with a lower PWV at 17 years: β −0.06 (95 % CI −0.12, −0.01). No other associations were observed. In conclusion, an Obesogenic dietary pattern in childhood (7–10 years) was related to increased arterial stiffness, while Mediterranean-style and anti-inflammatory diets were related to decreased arterial stiffness in adolescence. This highlights the importance of establishing healthy dietary habits early in life to protect against vascular damage.
Automated precision beekeeping for accessing bee brood development and behaviour using deep CNN
Authors: Rathore Neha , Agrawal Dheeraj 
Published: January 2024

AbstractBees play a significant role in the health of terrestrial ecosystems. The decline of bee populations due to colony collapse disorder around the world constitutes a severe ecological danger. Maintaining high yield of honey and understanding of bee behaviour necessitate constant attention to the hives. Research initiatives have been taken to establish monitoring programs to study the behaviour of bees in accessing their habitat. Monitoring the sanitation and development of bee brood allows for preventative measures to be taken against mite infections and an overall improvement in the brood's health. This study proposed a precision beekeeping method that aims to reduce bee colony mortality and improve conventional apiculture through the use of technological tools to gather, analyse, and understand bee colony characteristics. This research presents the application of advanced digital image processing with computer vision techniques for the visual identification and analysis of bee brood at various developing stages. The beehive images are first preprocessed to enhance the important features of object. Further, object is segmented and classified using computer vision techniques. The research is carried out with the images containing variety of immature brood stages. The suggested method and existing methods are tested and compared to evaluate efficiency of proposed methodology.
Cloning and spatio-temporal expression of CsKr-h1 encoding the juvenile hormone response gene in Coccinella septempunctata L
Authors: Cheng Ying , Zhou Yuhang , Li Fengliang 
Published: January 2024

AbstractThe gene encoding juvenile hormone response (Krüppel homolog1, Kr-hl) in Coccinella septempunctata was investigated by cloning and analysing expression profiles in different developmental stages and tissues by quantitative real-time polymerase chain reaction (PCR). C. septempunctata Kr-hl (CsKr-hl) encoded a 1338 bp open reading frame (ORF) with a predicted protein product of 445 amino acids; the latter showed high similarity to orthologs in other species and contained eight highly-conserved Zn-finger motifs for DNA-binding. CsKr-hl was expressed in different developmental stages of C. septempunctata. The expression levels of CsKr-hl in eggs, 2nd, 3rd, 4th instar larvae, and pupa were 3.31, 2.30, 7.09, 0.58, and 7.48 times the number of 1st instar larvae, respectively. CsKr-hl expression levels in female adults gradually increased at 25–30 days and were significantly higher than expression at 1–20 days. CsKr-hl expression in 20–30 days-old male adults was significantly higher than males aged 1–15 days. CsKr-hl expression levels in heads of male and female adults were significantly higher than expression levels in the thorax, adipose, and reproductive system. Interestingly, CsKr-hl expression levels in the adipose and reproductive system of female adults were significantly higher than in adult male corresponding organs, which suggest that CsKr-hl plays an important role in regulating reproductive development in C. septempunctata.
Life table parameters and digestive physiology of Aulacophora lewisii Baly (Coleoptera: Chrysomelidae) on three Luffa acutangula (L.) Roxb. (Cucurbitaceae) cultivars
Authors: Das Susmita , Kumbhakar Sanoj , Debnath Rahul , Barik Anandamay 
Published: January 2024

AbstractAulacophora lewisii Baly (Coleoptera: Chrysomelidae) is an important pest of Luffa acutangula (L.) Roxb. (Cucurbitaceae) in India. Larvae of A. lewisii feed on the roots, while adults consume leaves of L. acutangula. In the current study, effects of three L. acutangula cultivars (Abhiskar, Debsundari, and Jaipur Long) on the life table parameters by age-stage, two-sex approach, and key digestive enzymatic activities (amylolytic, proteolytic, and lipolytic) of the larvae and adults of A. lewisii were determined. Further, nutrients (total carbohydrates, proteins, lipids, amino acids, and nitrogen content) and antinutrients (total phenols, flavonols, and tannins) present in the roots and leaves of three cultivars were estimated. The development time (egg to adult emergence) was fastest and slowest on Jaipur Long (31.80 days) and Abhiskar (40.91 days), respectively. Fecundity was highest and lowest on Jaipur Long (279.91 eggs) and Abhiskar (137.18 eggs), respectively. The intrinsic rate of increase (r) was lowest on Abhiskar (0.0511 day−1) and highest on Jaipur Long (0.0872 day−1). The net reproductive rate (R0) was lowest on Abhiskar (23.32 offspring female−1). The mean generation time (T) was shortest on Jaipur Long (52.59 days) and longest on Abhiskar (61.58 days). The amylolytic, proteolytic, and lipolytic activities of larvae and adults of A. lewisii were highest and lowest on Jaipur Long and Abhiskar, respectively. The lower level of nutrients and higher level of antinutrients influenced higher larval development time and lower fecundity of A. lewisii on Abhiskar than other cultivars. Our results suggest that Abhiskar cultivar could be promoted for cultivation.
The mental health of Australians bereaved during the first two years of the COVID-19 pandemic: a latent class analysis
Authors: Maccallum F. , Breen L. J. , Phillips J. L. , Agar M. R. , Hosie A. , Tieman J. , DiGiacomo M. , Luckett T. , Philip J. , Ivynian S. , Chang S. , Dadich A. , Grossman C. H. , Gilmore I. , Harlum J. , Kinchin I. , Glasgow N. , Lobb E. A. 
Published: January 2024

AbstractBackgroundThe COVID-19 pandemic disrupted many areas of life, including culturally accepted practices at end-of-life care, funeral rites, and access to social, community, and professional support. This survey investigated the mental health outcomes of Australians bereaved during this time to determine how these factors might have impacted bereavement outcomes.MethodsAn online survey indexing pandemic and bereavement experiences, levels of grief, depression, anxiety, and health, work, and social impairment. Latent class analysis (LCA) was used to identify groups of individuals who shared similar symptom patterns. Multinomial regressions identified pandemic-related, loss-related, and sociodemographic correlates of class membership.Results1911 Australian adults completed the survey. The LCA identified four classes: low symptoms (46.8%), grief (17.3%), depression/anxiety (17.7%), and grief/depression/anxiety (18.2%). The latter group reported the highest levels of health, work, and social impairment. The death of a child or partner and an inability to care for the deceased due to COVID-19 public health measures were correlated with grief symptoms (with or without depression and anxiety). Preparedness for the person's death and levels of pandemic-related loneliness and social isolation differentiated all four classes. Unemployment was associated with depression/anxiety (with or without grief).ConclusionsCOVID-19 had profound impacts for the way we lived and died, with effects that are likely to ricochet through society into the foreseeable future. These lessons learned must inform policymakers and healthcare professionals to improve bereavement care and ensure preparedness during and following future predicted pandemics to prevent negative impacts.