bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary Zinc Limitation Dictates Lifespan and Reproduction Trade‐Offs of Drosophila Mothers
Authors: Sarmah Sweta , Truong Hannah Thi‐Hong Hanh , McColl Gawain , Burke Richard , Mirth Christen K. , Piper Matthew D. W. 
Published: February 2025

ABSTRACTDietary metal ions significantly influence the lifespan and reproduction of Drosophila females. In this study, we show that not adding any of the metal ions to the diet adversely affects reproduction and lifespan. By contrast, food with no added Zn negatively impacts reproduction but does not adversely affect maternal lifespan, indicating it can dictate resource reallocation between key fitness traits. Specifically, it indicates that female flies stop producing eggs to conserve their body Zn for somatic maintenance. Although these data show that flies can sense varying dietary Zn levels to adjust their physiology, they cannot maximise egg production when faced with a choice between food with no added Zn or food with sufficient Zn to support maximum reproduction. Nonetheless, they can choose to preferentially oviposit on Zn‐containing food, perhaps indicating a strategy to assure offspring survival. We also uncovered a role for the white gene in sustaining high levels of egg viability when Zn is diluted in the diet. These insights into the role of dietary metal ions, particularly Zn, point to a central role for these dietary micronutrients to indicate environmental quality and so govern trade‐offs between lifespan and reproduction in flies.
Understanding Post‐Release Dispersal and Habitat Selection Helps Refine Management of Translocated Populations
Authors: Stone Zoe , Armstrong Doug , Parker Kevin 
Published: February 2025

ABSTRACTTranslocation outcomes in connected habitats are often uncertain, as individuals dispersing outside managed areas are exposed to threats. Post‐release monitoring can reduce uncertainty by revealing how dispersal and habitat selection influences establishment and population growth which inform future translocations. We undertook post‐release monitoring to identify habitat selection patterns following a translocation of toutouwai (North Island robin, Petroica longipes) to a large, contiguous forest habitat. Post‐release monitoring aimed to estimate survival, dispersal, and territory establishment to inform management decisions and future release site selection. We created species distribution models using monitoring data to identify differences in habitat selection during the post‐release dispersal and territory establishment phases. Toutouwai dispersed across 1312 ha but established territories within only 113 ha and 1 km from the release location. Site fidelity was higher than predicted, and there was no difference in dispersal or habitat selection across demographic groups. Critically, high site fidelity suggested that the extent of managed habitat was sufficient to protect dispersing individuals. Habitat selection preferences were stronger during territory establishment and were associated with lower slopes, higher water deficit and proximity to water reservoirs. Species distribution modelling allowed for predictions of high‐quality core habitat where dispersal and territory establishment were more likely, resulting in targeted management to improve population growth. Our results show that initial dispersal in connected habitats may be much larger than suggested by territory data alone, and that management may need to protect larger areas to support successful establishment. We demonstrate how effective post‐release monitoring can inform predictions of habitat quality and dispersal and guide management actions to improve translocations outcomes.
Pushpin memoir: Making meaning out of murder
Authors: Sullivan Noelle 
Published: February 2025

AbstractIn 2018, my brother Adam Colquhoun, nicknamed “Stretch,” was killed in a bar in Calgary, Alberta by a man he barely knew. Stretch was the kind of person society finds convenient to discard. His history of theft, illicit drug dealing, mental illness, addiction, and homelessness made his humanity “undesirable.” Nevertheless, lessons Stretch learned painfully over his lifetime had something to teach us. In 2016, he told me he wanted someone to write his biography. This paper explores how my brother's murder caused me to collapse the binary between personal and professional, and embrace memoir—a process differing substantially from writing ethnography. I thought of my approach as “pushpin memoir,” akin to a detective board where investigators stick bits of evidence in the aftermath of a crime. Pushpins comprise unusual, standout moments we hold onto among the quotidian we often forget. Meanings gleaned from Stretch's life allowed me to rethink the future of the man who killed him, and thus imagine broader possibilities for people whose actions cause irreparable harm. I ponder how centering the experiencing self feels crucial to future endeavors to write an anthropology that matters to broader publics I hope to engage.
Advances in primary eye care and ophthalmic imaging contribute to earlier detection of uveal melanoma
Authors: Bjertnæs Vilde , Hatling Anna Dalseng , Haugen Olav H. , Krohn Jørgen 
Published: February 2025

AbstractPurposeTo report how the evolving role of optometrists in primary eye care and advances in ophthalmic imaging have affected diagnosis and management of posterior uveal melanoma (UM).MethodsRetrospective, single‐centre cohort study of patients diagnosed with UM from 1993 to 2022 in Bergen, Norway.ResultsFour hundred nine patients were included, and comparisons were made between those diagnosed from 1993 to 2007 and from 2008 to 2022. The median tumour diameter decreased from 13.3 to 11.3 mm (p = 0.002), and the median tumour thickness decreased from 6.9 to 4.5 mm (p < 0.001). The median distance from the tumour border to the optic disc and to the foveola increased from 3.5 to 4.5 mm (p = 0.011), and from 3.0 to 4.0 mm (p < 0.001), respectively. Two hundred sixty‐two patients (64%) experienced symptoms associated with UM, with a median duration of 152.5 and 81 days in the first and second half of the study period, respectively (p < 0.001). The median best corrected visual acuity at diagnosis improved from 0.5 logMAR (Snellen equivalent, 6/19) in the first half to 0.2 logMAR (Snellen equivalent, 6/9.5) in the second half of the period (p < 0.001). The proportion of asymptomatic patients was 23.5% in the first and 41.9% in the second half of the period (p < 0.001). The proportion of UMs incidentally detected by optometrists increased from 3.0% to 18.1% (p = 0.009), and the proportion of UMs incidentally detected by fundus photography increased from 1.5% to 18.1% (p < 0.001).ConclusionsThe temporal changes in patient and tumour characteristics suggest that UMs are now being diagnosed at an earlier stage. This may in part be attributed to the introduction of widefield fundus cameras and increased opportunistic screening of asymptomatic patients.
CORONIS symposium 2023: Scientific and clinical frontiers in ocular surface innervation
Authors: Galor Anat , Gallar Juana , Acosta M. Carmen , Meseguer Víctor , Benítez‐Del‐Castillo José Manuel , Stachs Oliver , Szentmáry Nóra , Versura Piera , Müller‐Lierheim Wolfgang G. K. , Belmonte Carlos , Pujol‐Martí Jesús 
Published: February 2025

AbstractThe 5th CORONIS Symposium, held during the 2023 Congress of the European Association for Vision and Eye Research (EVER), highlighted the growing importance of ocular surface innervation in eye surface disorders. This article summarises the insights and perspectives shared during the symposium, which focused on the clinical relevance of ocular surface innervation, as well as on the development of innovative diagnostic and therapeutic approaches for ocular surface pathologies linked to disturbed sensory innervation. Through robust interdisciplinary collaborations, these developments hold great potential to improve patient outcomes and quality of life.
Simulation‐Based Echocardiography Training Enhanced Competence and Self‐Confidence in Neonatal Residents
Authors: Blanchetière Amélie , Guillouët Erwan , Favrais Géraldine , Lardennois Caroline , Bellot Anne , Savey Baptiste 
Published: February 2025

ABSTRACTAimMastering echocardiography scans is essential in neonatology, but bedside training can be difficult due to the instability of neonatal patients. The aim of this study was to demonstrate the benefits of simulation‐based training for residents learning echocardiography.MethodsThis multicentre randomised controlled trial involved residents from three French neonatology departments. The authors compared a control group with theoretical and bedside training and a simulation group with additional simulation‐based training. Evaluations were conducted three and 6 months after training using two scoring methods: a reference score for the quality of the echocardiographic sections and a custom‐made score for recognising anatomical structures.ResultsWe randomised 52 residents from 1 May 2021 to 31 May 2023. After 3 months, the residents in the simulation group achieved a higher mean score than the residents in the control group for both the reference score (11.5 ± 2.3 points vs. 7.4 ± 3.4 points, p < 0.001) and the custom‐made score (25.8 ± 5.3 points vs. 16.9 ± 7.8 points, p < 0.001). The difference remained significant at 6 months and the custom‐made score showed good agreement with the reference score.ConclusionSimulation‐based training was a valuable approach for training neonatal residents to perform echocardiography and more extensive training courses should be developed.Trial RegistrationClinicalTrials.gov identifier: NCT06442683
Greater Parent–Child Brain Synchronisation During Printed Book Versus Screen Reading Using Hyperscanning Electroencephalograph Data
Authors: Jomaa Fauzi , Ebraheem Fatma , Horowitz‐Kraus Tzipi 
Published: February 2025

ABSTRACTAimThe differences between parent–child joint attention while reading together from a screen versus from a paper are unknown. The current study aimed to determine if parent–child brains synchronise differently during screen‐based versus printed paper‐based, in other words, a book reading.MethodsThe study was carried out in 2022 in Israel. Cohorts were recruited via posted ads. Cognitive and behavioural measures were assessed using standardised tests. In addition, two reading sessions were administered by the parent, one on the screen and another using a book, while electroencephalograph data captured their brain synchronisation. The difference in brain synchronisation between the conditions was correlated with behavioural measures.ResultsOf the 49 parent–child pairs age 3.94 years ± 0.751; 24 females who participated in language‐based tasks for this study, electroencephalograph data from 11 dyads showed a higher brain‐to‐brain synchronisation during printed‐based reading compared to screen‐based reading was found and associated with the child's verbal and cognitive abilities.ConclusionPrinted‐paper reading fosters a higher parent–child neural synchronisation and cognitive engagement compared to screen‐based reading.
Childbirth Experience, Mistreatment, and Migrant Status: A Retrospective Cross‐Sectional Study
Authors: Mangindin Edythe L. , Gottfreðsdóttir Helga , Stoll Kathrin , Cadée Franka , Lárusdóttir Elín Inga , Swift Emma M. 
Published: February 2025

ABSTRACTIntroductionChildbirth experience can affect women's long‐term health and well‐being. However, there is limited knowledge on whether migrant status affects woman's experience during childbirth. We aimed to answer the following research questions: (1) Is there a difference in childbirth experience between migrant and native‐born women in Iceland; and (2) Are migrant women more likely to experience mistreatment in childbirth compared to native‐born women in Iceland?MethodsAn online survey was developed including the Childbirth Experience Questionnaire 2 to assess overall childbirth experience, and descriptive analysis and linear regression were conducted to determine differences between migrant and native‐born women in Iceland. The mistreatment by care providers in childbirth indicators were used to evaluate mistreatment in childbirth, and frequencies and logistic regression were conducted. Both regression models were adjusted for sociodemographic and obstetric factors.ResultsA total of 1365 women participated. Migrant women reported statistically significantly lower scores for birth experience compared to native‐born women (F [12, 1352] = 23.97, p < 0.001). There was no statistical difference between groups regarding mistreatment in childbirth. One in four of all women reported at least one form of mistreatment.ConclusionThis study suggests that there are areas in maternity care that can be improved upon, particularly in providing care for migrant women and addressing mistreatment in childbirth for all. Our results suggest further research in this area as well as evaluation of maternity systems, training in cultural competency and effective communication.
Explaining the self‐regulatory role of affect in identity theory: The role of self‐compassion
Authors: Strachan Shaelyn M. , Kullman Sasha M. , Dobrovolskyi Mark , Vega Vianney Z. , Yarema Alexandra , Patson Caity 
Published: February 2025

AbstractObjectivesAccording to Stets and Burke's Identity Theory, people experience negative affect when their behaviour deviates from their identity standards, which drives the regulation of identity‐relevant behaviour. Guilt and shame represent unique forms of negative affect. Self‐compassion may influence guilt and shame responses about identity‐behaviour inconsistencies. Relative to exercise identity, we examined the associations between (1) guilt and shame, behavioural intentions, and perceptions of identity‐behaviour re‐alignment after an identity‐inconsistent situation and (2) whether self‐compassion moderates the relationship between these forms of negative affect and both behavioural intentions and identity‐behaviour re‐alignment.DesignProspective, online, quantitative.MethodsN = 274 exercisers (Mage = 32.5 years, SDage = 10.8 years, 50.2% women) who engaged in less exercise in the past week than their identity standard were recruited from Prolific.com. At baseline, self‐compassion, state and trait guilt and shame, and exercise intentions were measured. One week later, participants reported the extent to which their past week's exercise aligned with their identity standard (i.e., identity‐consistent perceptions).ResultsNeither state shame nor guilt related to exercise intentions nor identity‐consistent perceptions. Self‐compassion moderated the relationship between state guilt and identity‐consistent perceptions (b = 2.524, SE = .975, t = 2.588, p = .010); state guilt was related to identity‐behaviour consistency when self‐compassion was high, but not when it was low. No other moderations were significant.ConclusionsThis study adds nuance to Identity Theory and its propositions about negative affect and self‐regulation; self‐compassion may create the conditions necessary for negative affect to drive identity‐relevant behaviour as proposed by identity theory.
Thiamine deficiency aggravates experimental colitis in mice by promoting glycolytic reprogramming in macrophages
Authors: Pan Xiaohua , Ren Zhengnan , Liang Wenjie , Dong Xiaoliang , Li Jiahong , Wang Lili , Bhatia Madhav , Pan Li‐Long , Sun Jia 
Published: February 2025

AbstractBackground and PurposeInflammatory bowel disease (IBD) is closely associated with immune dysfunction, where nutrient‐mediated metabolic flux dictates immune cell fate and function. Thiamine is a central water‐soluble vitamin involved in cellular energy metabolism, and its deficiency has been reported in IBD patients. However, whether thiamine deficiency is a cause or consequence of IBD pathogenesis remains unclear. The current study aimed to reveal the immunometabolic regulation of macrophages and underlying mechanism of thiamine deficiency in colitis development.Experimental ApproachThiamine deficiency was induced in C57BL/6 mice and bone marrow‐derived macrophages (BMDMs), by administering a thiamine‐deficient diet/medium together with pyrithiamine hydrobromide. The frequency of macrophage phenotypes and their intracellular metabolism were detected using flow cytometry and non‐targeted metabolomics, respectively.Key ResultsThiamine deficiency aggravated ulcerative colitis in mice and promoted the infiltration of proinflammatory M1 macrophages in colonic lamina propria. Our mechanistic study revealed that thiamine deficiency impaired pyruvate dehydrogenase (PDH) activity, thereby reprogramming cellular glucose metabolism to enhance glycolysis and lactic acid accumulation in M1 macrophages. Using a well‐established PDH inhibitor (CPI‐613) and lactic acid dehydrogenase inhibitor (galloflavin), we further demonstrated that PDH inhibition mimics, while lactate dehydrogenase inhibition partially rescues, thiamine deficiency‐induced proinflammatory macrophage infiltration and experimental colitis in mice.Conclusion and ImplicationsOur study provides evidence linking thiamine deficiency with proinflammatory macrophage activation and colitis aggravation, suggesting that monitoring thiamine status and adjusting thiamine intake is necessary to protect against colitis.
Genomic Testing in Adults With Undiagnosed Rare Conditions: Improvement of Diagnosis Using Clinical Exome Sequencing as a First‐Tier Approach
Authors: Petillo Roberta , De Maggio Ilaria , Piscopo Carmelo , Chetta Massimiliano , Tarsitano Marina , Chiriatti Luigi , Sannino Elvira , Torre Serena , D'Antonio Marcella , D'Ambrosio Paola , Rambaldi Marco , Cioce Maria , De Stefano Valentina , Parisi Maria Rita , Telese Antonella , Oro Maria , Rivieccio Maria , Radio Francesca Clementina , Mancini Cecilia , Niceta Marcello , Cordeddu Viviana , Bruselles Alessandro , Mammì Corrado , Dattola Adele , Fioretti Tiziana , Esposito Gabriella , Novelli Antonio , Tessitore Alessandro , Tessa Alessandra , Santorelli Filippo Maria , Iolascon Achille , Monica Matteo Della , Tartaglia Marco , Priolo Manuela 
Published: February 2025

ABSTRACTAdult patients with undiagnosed genetic disorders suffer most from diagnostic delay and seldom appear in cohort studies investigating the diagnostic yield in medical genetic clinical practice. Here we present the results of the diagnostic activity performed in a referral center on 654 consecutive, unselected adult subjects presenting with molecularly unsolved conditions. More than 50% of the referred individuals were affected by syndromic or isolated intellectual disability. Different molecular approaches, including clinical/whole exome sequencing (CES/WES), chromosomal microarray analysis (CMA), and/or targeted gene or gene panel sequencing were used to analyze patients' DNA. Definitive diagnosis was obtained in over 30% of individuals. The most sensitive methodology was CES/WES, which allowed us to reach a diagnosis in over 50% of the 162 solved cases. Despite the great variety of clinical presentations, our results represent a reliable picture of the “real world” daily routine in an outpatient medical genetics clinic dedicated to diagnostic activity, and contribute to better understand the great value of a definitive molecular diagnosis in adults, either for the affected individuals and their families. This retrospective analysis demonstrates the importance of adopting a genomic‐first approach within the diagnostic process for adults affected with unsolved rare conditions.
Full‐Arch Digital Implant Impression Trueness: An in Vivo Study
Authors: Rutkūnas Vygandas , Kuleš Daniel , Revilla‐León Marta , Akulauskas Mykolas , Auškalnis Liudas , Gendvilienė Ieva 
Published: February 2025

ABSTRACTObjectivesTo evaluate the trueness of maxillary and mandibular full‐arch implant digital impressions with and without additional reference objects.Material and MethodsSeventeen maxillary and 6 mandibular arches with four implants were randomly divided into a group with additional reference objects (wRO group, n = 12) and without them (woRO group, n = 11) and scanned with an intraoral scanner (IOS). For each jaw, the control model (CM) data was obtained to evaluate the deviations in the scan body positions in the wRO and woRO groups. CM and IOS data sets were imported into metrology software for distance and angle measurements between scan body pairs 1–2, 1–3, and 1–4. Unsigned values of distance and angle data differences between CM and IOS groups were used for further analysis.ResultsThe highest statistically significant (p < 0.05) differences between distance and angulation measurements of the maxilla and mandible were observed in the 1–4 scan body pair group (136 ± 95 μm, 0.95° ± 0.79°; 362 ± 233 μm, 2.22° ± 2.11°, respectively). Distance deviations of the mandible were higher in all scan body pair groups. The woRO group had higher distance and angulation unsigned trueness values than in the wRO group, especially in the 1–4 scan body pair (224 ± 183 μm, 1.77° ± 1.76°;169 ± 162 μm, and 0.83° ± 0.57°, respectively).ConclusionsIOS for the full‐arch cases had statistically and clinically significant distance and angle deviations. Higher deviations were observed in the single mandibular scan body pair. The tendency of diminished distance and angular deviations with the utilization of the additional reference objects was observed, though these differences were not statistically significant.
Mild cryoinjury in zebrafish fin induces regenerative response without blastema formation
Authors: Yoshida Takafumi , Kawakami Atsushi 
Published: February 2025

AbstractPrevious studies have shown that tissue regeneration induces expression of genes that play important roles in regeneration. Recently, several studies have identified regeneration‐response enhancers (RREs) that activate gene expression by tissue injury. Particularly, we showed that RREs contain two transcription factor‐binding motifs: a bHLH transcription factor‐binding motif, an E‐box, and an AP‐1/bZIP transcription factor‐binding motif, a 12‐O‐Tetradecanoylphorbol 13‐acetate response element (TRE). However, the triggers and subsequent signals generated by injury are still unclear. In this study, we analyzed RRE activation using various injury models. Although inter‐ray incisions and skin exfoliation injuries did not activate RREs or regeneration genes, the fin puncture injury activated RREs and several regeneration‐response genes. After fin puncture injury, msxc was activated only on the proximal side of the hole where blastema‐like tissue was formed, whereas RREs, junbb, and fibronectin 1b (fn1b) were activated on both the proximal and distal sides, implying that activation of RREs, junbb, and fn1b is independent of blastema formation. Here, we also established a mild cryoinjury method. After this injury, transient vascular destruction, an increase in cell death, and an accumulation of myeloid cells were observed; however, no major morphological damage was observed. Importantly, msxc was not induced by cryoinjury, whereas fn1b, junbb, and 1.8 k RRE (−1.8 kb promoter of fn1b) were activated, suggesting that cryoinjury induces the responses of fn1b, junbb, and 1.8 k RRE without forming the blastema. Thus, our study shows that the cryoinjury model and the RRE transgenic (Tg) zebrafish may provide a useful platform for exploring injury signals.