bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bodies in Pain: Early Modern Suicide by Proxy
Authors: Berger Andreas 
Published: December 2023

Abstract
This article examines early modern suicide by proxy in terms of the experience of pain. ‘Suicide by proxy’ refers to committing a capital crime in order to bring about one’s own death by execution. Exploring the history of prolonged pain typically associated with suicide by proxy, the article argues that suicide by proxy is primarily a story about pain. The analysis here follows the story of Sara Stähelin and her attempt to use suicide by proxy as a way to liberate herself from her hurting body and mind, to receive comfort and compassion from her estranged community and—most importantly—to save her soul from eternal damnation. Understanding suicide by proxy as a story of how early modern pain could materialize offers a new and fruitful approach to the study of early modern pain and its mediation between culture and body.
The sectoral trade losses from financial crises
Authors: Atsebi Jean-Marc B , Combes Jean-Louis , Minea Alexandru 
Published: December 2023

Abstract
The “Great Trade Collapse” triggered by the 2008-2009 crisis calls for a careful assessment of the trade losses from financial crises. We adopt a more detailed perspective by looking at the response of different types of trade (i.e. consumption, intermediate, capital goods, and business services) following various types of financial crises (i.e. debt, banking, and currency crises) in 41 emerging markets. Estimations performed in the 1980-2019 period using a combination of impact assessment and local projections to capture a causal dynamic effect running from financial crises to the trade activity show that the collapse of total trade is long-lasting and mainly driven by the fall of intermediate goods and to some extent capital goods, while trade in consumption goods and business services is more resilient to crises. Therefore, financial crises could lead to considerable disruption of global value chains, as observed during the Global Financial Crisis (GFC), and easily spill over from one country to another through trade linkages. The examination of heterogeneity reveals that total and sectoral trade is more severely impacted in countries with a lower share of manufacturing exports, less diversified exported products, and trading partners, with lower demand from trading partners and when associated with a deterioration of the domestic and external financial conditions and sudden stops. By contributing to the understanding of the trade effects of financial crises, our analysis provides insightful support for the design and implementation of policies aimed at coping with these effects.
Preweaning piglet survival on commercial farms
Authors: Sanz-Fernández Santos , Díaz-Gaona Cipriano , Casas-Rosal José C , Alòs Nuria , Tusell Llibertat , Quintanilla Raquel , Rodríguez-Estévez Vicente 
Published: December 2023

AbstractPreweaning piglet mortality (PWM), a trait highly related to litter size, is one of the main concerns associated with productive efficiency and animal welfare in commercial pig farms. The objectives of this work were to study piglet survival at the farm level, to establish a survival rate (SR) as a target indicator to be improved, and to model it based on other reproductive parameters. Analyzed data corresponded to 580 Spanish commercial farms with a total inventory of 809,768 sows. These farms showed a mean SR of 85.70% piglets born alive (BA), which decreased to 81.81% when total piglets born (TB) were considered. The SR was strongly associated with prolificacy (P &lt; 0.01), the parities with the highest prolificacy being those that had the lowest SR. Thus, the highest correlations were for the SR of piglets BA in the third and fourth parities (r = −0.460 and r = −0.452, respectively, P &lt; 0.01), and for the SR of piglets TB in the fourth parity (r = −0.546, P &lt; 0.01), which was the one with the highest prolificacy. The values corresponding to the quartile of farms with the highest SR within the most productive farms were established as targets to be improved, which were ≥88.5% of piglets BA and 83.2% of piglets TB. Nevertheless, the direct associations shown between the piglet’s survival and prolificacy and other productive factors, such as the age of piglets at weaning, the farrowings per sow and year and the farrowing interval, suggest the convenience of modeling the risk of PWM on farms to have its own target of survival index to be improved.
Treatment of Necrotizing Fasciitis With NovoSorb Biodegradable Temporizing Matrix and RECELL Autologous Skin Cell Suspension: A Case Series
Authors: Austin Cindy L , Sodade Oluwafolaranmi E , Harrison Brody , Causa Krisi 
Published: December 2023

Abstract
This case series reviews the management of 2 patients who developed a rare, aggressive soft tissue infection, necrotizing fasciitis treated with a synthetic polyurethane dermal regenerative template, Biodegradable Temporizing Matrix (NovoSorb BTM) in conjunction with an off-label use of RECELL device applying autologous skin cell suspension and a split-thickness skin graft for reconstruction. The clinical relevance describes a non-traditional patient’s course of treatment and clinical outcome using BTM and RECELL for necrotizing fasciitis. The 2 patients survived with acceptable outcomes and timely healing despite a high chance of mortality and likely amputation secondary to the extensive surface area and anatomical location of the infection.
From forests to fields: investigating Culicoides (Diptera: Ceratopogonidae) abundance and diversity in cattle pastures and adjacent woodlands
Authors: Steele Cassandra H , McDermott Emily G 
Published: December 2023

Abstract
Culicoides Latreille (Diptera: Ceratopogonidae) biting midges are hematophagous flies that feed on wild and domestic ruminants. They can transmit arboviruses, such as bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), which circulate in the United States. Larvae occupy a range of aquatic and semiaquatic habitats, and disperse short distances from their development sites. In the southeastern United States, there are limited studies on the abundance and diversity of Culicoides in wooded and adjacent livestock pasture habitats. In this study, we characterized Culicoides diversity and abundance within these distinct habitat types. BG-Sentinel and CDC miniature suction traps baited with CO2 or UV-light were placed in wooded and pasture habitats at 2 locations on a university beef farm in Savoy, Arkansas. Traps were set once per week for 9 wk during August–October of 2021 and 2022. Fifteen species were collected during this study, and the 2 most abundant species were Culicoides haematopotus Malloch and Culicoides stellifer Coquillett. There was a significant effect of site and location on C. haematopotus collections, and a significant effect and interaction of site and trap on C. stellifer collections. In the woods, significantly more C. stellifer were collected from CDC–UV traps, while in the pasture significantly more were collected in CDC–CO2 traps. These data suggest that C. stellifer, a putative vector of BTV and EHDV in the southeast, may be traveling into the pasture to host-seek, while C. haematopotus remains primarily in wooded areas. This study reveals community differences between these habitat types and implications for Culicoides control.
Pasa: leveraging population pangenome graph to scaffold prokaryote genome assemblies
Authors: Do Van Hoan , Nguyen Son Hoang , Le Duc Quang , Nguyen Tam Thi , Nguyen Canh Hao , Ho Tho Huu , Vo Nam S , Nguyen Trang , Nguyen Hoang Anh , Cao Minh Duc 
Published: December 2023

Abstract
Whole genome sequencing has increasingly become the essential method for studying the genetic mechanisms of antimicrobial resistance and for surveillance of drug-resistant bacterial pathogens. The majority of bacterial genomes sequenced to date have been sequenced with Illumina sequencing technology, owing to its high-throughput, excellent sequence accuracy, and low cost. However, because of the short-read nature of the technology, these assemblies are fragmented into large numbers of contigs, hindering the obtaining of full information of the genome. We develop Pasa, a graph-based algorithm that utilizes the pangenome graph and the assembly graph information to improve scaffolding quality. By leveraging the population information of the bacteria species, Pasa is able to utilize the linkage information of the gene families of the species to resolve the contig graph of the assembly. We show that our method outperforms the current state of the arts in terms of accuracy, and at the same time, is computationally efficient to be applied to a large number of existing draft assemblies.
Isolation and detection of DNA–protein crosslinks in mammalian cells
Authors: Torrecilla Ignacio , Ruggiano Annamaria , Kiianitsa Kostantin , Aljarbou Ftoon , Lascaux Pauline , Hoslett Gwendoline , Song Wei , Maizels Nancy , Ramadan Kristijan 
Published: December 2023

Abstract
DNA–protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, DPCs create obstacles that disturb DNA replication, transcription and DNA damage repair, ultimately leading to genome instability. The persistence of DPCs is associated with premature ageing, cancer and neurodegeneration. In mammalian cells, the repair of DPCs mainly relies on the proteolytic activities of SPRTN and the 26S proteasome, complemented by other enzymes including TDP1/2 and the MRN complex, and many of the activities involved are essential, restricting genetic approaches. For many years, the study of DPC repair in mammalian cells was hindered by the lack of standardised assays, most notably assays that reliably quantified the proteins or proteolytic fragments covalently bound to DNA. Recent interest in the field has spurred the development of several biochemical methods for DPC analysis. Here, we critically analyse the latest techniques for DPC isolation and the benefits and drawbacks of each. We aim to assist researchers in selecting the most suitable isolation method for their experimental requirements and questions, and to facilitate the comparison of results across different laboratories using different approaches.
Postoperative cerebellar mutism syndrome is an acquired autism-like network disturbance
Authors: Suresh Hrishikesh , Morgan Benjamin R , Mithani Karim , Warsi Nebras M , Yan Han , Germann Jürgen , Boutet Alexandre , Loh Aaron , Gouveia Flavia Venetucci , Young Julia , Quon Jennifer , Morgado Felipe , Lerch Jason , Lozano Andres M , Al-Fatly Bassam , Kühn Andrea A , Laughlin Suzanne , Dewan Michael C , Mabbott Donald , Gorodetsky Carolina , Bartels Ute , Huang Annie , Tabori Uri , Rutka James T , Drake James M , Kulkarni Abhaya V , Dirks Peter , Taylor Michael D , Ramaswamy Vijay , Ibrahim George M 
Published: December 2023

Abstract

Background
Cerebellar mutism syndrome (CMS) is a common and debilitating complication of posterior fossa tumor surgery in children. Affected children exhibit communication and social impairments that overlap phenomenologically with subsets of deficits exhibited by children with Autism spectrum disorder (ASD). Although both CMS and ASD are thought to involve disrupted cerebro-cerebellar circuitry, they are considered independent conditions due to an incomplete understanding of their shared neural substrates.

Methods
In this study, we analyzed postoperative cerebellar lesions from 90 children undergoing posterior fossa resection of medulloblastoma, 30 of whom developed CMS. Lesion locations were mapped to a standard atlas, and the networks functionally connected to each lesion were computed in normative adult and pediatric datasets. Generalizability to ASD was assessed using an independent cohort of children with ASD and matched controls (n = 427).

Results
Lesions in children who developed CMS involved the vermis and inferomedial cerebellar lobules. They engaged large-scale cerebellothalamocortical circuits with a preponderance for the prefrontal and parietal cortices in the pediatric and adult connectomes, respectively. Moreover, with increasing connectomic age, CMS-associated lesions demonstrated stronger connectivity to the midbrain/red nuclei, thalami and inferior parietal lobules and weaker connectivity to the prefrontal cortex. Importantly, the CMS-associated lesion network was independently reproduced in ASD and correlated with communication and social deficits, but not repetitive behaviors.

Conclusions
Our findings indicate that CMS-associated lesions may result in an ASD-like network disturbance that occurs during sensitive windows of brain development. A common network disturbance between CMS and ASD may inform improved treatment strategies for affected children.
A population-based study of Helicobacter pylori: Does asymptomatic infection mean no gastroscopic lesions?
Authors: Cai Ting , Li Ying , Li Xin-meng , Chen Bing , Liang Lun-xi , Yuan Ling-zhi , Hu Hai , Zhang Ming-lin , Deng Ao-jian , Liu Xiao-ming , Wang Fen 
Published: December 2023

Abstract

Objectives
We determined the common clinical characteristics of patients infected with Helicobacter pylori (H. pylori) and investigated the relationship between H. pylori infection, and clinical symptoms, and gastroscopic manifestations. Our focus was specifically on the clinical manifestations in asymptomatic patients.

Methods
We obtained the physical examination data of patients who underwent the 14C urea breath test between January 2018 and December 2020 at our Hospital. Basic demographic data, questionnaire data on clinical symptoms, and clinical examination data of the patients were also collected, and the correlation analysis was performed.

Results
A total of 2863 participants were included in the study. The overall H. pylori infection rate was 26.30%. The clinical symptoms between H. pylori-positive patients and H. pylori-negative patients did not differ significantly (P &gt; .05). However, H. pylori-positive patients exhibited more severe gastroscopic manifestations (P &lt; .001). The 14C urea breath test disintegrations per minute (DPM) values in H. pylori-positive patients correlated with their serum pepsinogen and gastrin-17 levels. With an increase in the DPM value, more combinations of clinical symptoms appeared in the patients. Among H. pylori-positive patients, DPM levels in asymptomatic patients were lower than those in symptomatic patients (P &lt; .001). However, gastroscopic manifestations did not vary significantly between asymptomatic and symptomatic patients (P &gt; .05).

Conclusion
Patients infected with H. pylori showed no specific gastrointestinal symptoms. Patients with asymptomatic infection showed lower DPM levels, but their gastroscopic manifestations were similar to those of patients with symptomatic infection, and their lesions were more severe than H. pylori-negative people.
Developments in the United Arab Emirates impacting family offices and family wealth structures
Authors: Crivellaro Jacopo 
Published: December 2023

Abstract
This article examines the United Arab Emirate’s (UAE) evolving legislative landscape for family offices and family wealth structures, focusing on the 2022 UAE Family Business Law and the 2023 Dubai International Financial Centre Family Arrangements Regulations and their implications for the management and succession of family businesses, as well as the regulation of family offices in various financial free zones.
The Power of a Simple Question
Authors: Berry Arnold J. 
Published: December 2023

The Prevalence of Hepatitis B Markers in Anesthesia Personnel. By Berry AJ, Isaacson IJ, Hunt D, Kane MA. Anesthesiology 1984; 60:6–9
The prevalence of hepatitis B viral markers has increased in some groups of medical workers who are exposed to blood from patients carrying the virus, but this has not been studied critically in physicians and others who administer anesthesia. Physician anesthesiologists (M.D.) and nurse anesthetists and anesthesia assistants (non-M.D.) at four university-affiliated hospitals were evaluated for hepatitis B markers as determined by seropositivity for hepatitis B surface antigen, antibody to the hepatitis B surface antigen, or antibody to the hepatitis B core antigen. In the 86 subjects (38 M.D., 48 non-M.D.) who represented 80.4% of possible participants, the overall prevalence of serologic markers of hepatitis B was 23.3%. The frequency did not differ between M.D. (23.7%) and non-M.D. (22.9%) groups or between men (20.3%) and women (26.9%). Of 81 subjects who had no clinical history of hepatitis, 16 (19.8%) had positive serologic markers. The frequency of seropositivity increased with time since graduation from medical school (M.D.) or nursing school or college (non-M.D.). The prevalence of serologic markers of hepatitis B virus in this study of anesthesia personnel is five to eight times that of the general population but is similar to that of other medical workers who frequently are exposed to blood.
Chondroblastomas in Children and Young Adults: Revision of 55 Cases
Authors: Brunet Laia , Torner Ferran , Suñol Mariona , Martínez Judit , Gracia Isidre , Peiró Ana , Machado Pau 
Published: December 2023

Background:
Chondroblastomas are uncommon primary bone tumors localized in long bone epiphyses in children and young adults. The risk of metastasis is rare, but they have a high capacity for local recurrence. Surgical curettage with bone grafting or bone substitute is the preferred treatment.

Methods:
We performed an observational retrospective study of chondroblastomas treated in 2 hospitals in Barcelona from 1988 to 2018. We reviewed the location of the tumor, clinical presentation, imaging, histopathology, initial treatment, and cases of recurrence with a review of their treatment. We assessed the correlation between recurrence and index surgery, anatomic location, and certain histopathologic findings (presence of mitotic figures, necrosis, and positivity for protein S-100).

Results:
The series included 55 patients treated from 1988 to 2018, with ages ranging from 6 to 26, and a mean follow-up of 6.1 years (±3.7). The most common location was the distal femur metaphyseal/epiphyseal region. The most frequent clinical presentation was pain in the affected. Forty-five cases (81.8%) were treated with curettage of the tumor, and 4 cases (7.3%) with a wide resection. Forty-two cases (85.7%) received bone substitutes after curettage or resection. We found 5 cases of recurrence (9.1% recurrence rate); however, we could not find a statistically significant correlation between index surgery and recurrence (P=0.24), anatomic location and recurrence (P=0.49), or recurrence and histopathologic findings (mitotic figures, P=0.49; necrosis, P=0.60; positivity for protein S-100, P=0.52). In all the cases the treatment for the local recurrence was surgical, with a final healing rate of 100%.

Conclusions:
Chondroblastomas should be considered in children and adolescents when presenting with pain and an image suggestive of a tumoral lesion on plain x-ray, most frequently in epiphyses of long bones.
Surgical treatment is preferred, obtaining good results after curettage and bone substitute. Chondroblastomas are tumors with a high capacity for recurrence, therefore an adequate surgical technique and surgeon experience are paramount to achieve good outcomes.

Level of Evidence:
Level IV (case series). Therapeutic studies—investigating results or treatment.
Long-Term (>24 Months) Duration of Symptoms Negatively Impacts Patient-Reported Outcomes Following Anterior Cervical Discectomy and Fusion for Cervical Radiculopathy
Authors: Liu Eva , Persad Amit R.L. , Baron Nathan , Fourney Daryl R. 
Published: December 2023

Study design.
Retrospective cohort study.

Objective.
To investigate the impact of long symptom duration (>24 mo) on patient self-reported outcomes for pain, function, and quality of life following anterior cervical discectomy and fusion (ACDF) for cervical radiculopathy.

Summary of Background Data.
ACDF is an effective treatment to relieve the symptoms of cervical radiculopathy. However, there is no consensus on whether prolonged preoperative length of symptoms negatively impacts postoperative outcomes.

Methods.
This study included consecutive patients who underwent ACDF for cervical radiculopathy from May 1, 2012 to Dec 1, 2019 by a single surgeon. Patients were stratified by short (<24 mo) and long (>24 mo) duration of symptoms. Outcomes including visual analog scale (VAS) neck and arm, neck disability index (NDI), EuroQol-5D (EQ-5D), and overall state of health (EQ-VAS) were compared between cohort both for absolute values and percentage of patients achieving minimal clinically important difference.

Results.
A total of 111 consecutive patients were included in our study, including 59 patients in the short symptom duration group and 52 patients in the long symptom duration group. The mean age of the patients was 51.4±9.4 and 41 (36.9%) were female. The baseline VAS neck and arm, NDI, EQ-5D, and EQ-VAS were similar between groups. Patients in both long and short symptom duration groups had clinical improvement following surgery. However, patients with short symptom duration had better VAS Neck and EQ-5D outcomes, and were more likely to meet minimal clinically important difference for NDI, EQ-5D, or any outcome. Multivariate analysis confirmed symptom duration <24 months as an independent predictor for better patient-reported outcomes.

Conclusion.
We appreciated better clinical outcomes in patients with shorter symptom duration who received ACDF for cervical radiculopathy. On the basis of this data, we advocate for prompt treatment of cervical radiculopathy to avoid the potential for long-term impairment.

Level of Evidence:
Level 3.
LncRNA MALAT1-Targeting Antisense Oligonucleotide Ameliorates the AngII-Induced Vascular Smooth Muscle Cell Proliferation and Migration Through Nrf2/GPX4 Antioxidant Pathway
Authors: Liao Zili , Ni Zhonghan , Cao Jun , Liao Jin , Zhu Hengqing , Zhong Xiutong , Cao Gang , Huang Ling , Li Xiaoyue , Jiang Guojun , Pei Fang 
Published: December 2023

Abstract:
The high level of oxidative stress induced by angiotensin II (AngII) is the main pathophysiological process that promotes the proliferation and migration of vascular smooth muscle cells (VSMCs) and induces vascular remodeling. LncRNA metastasis-related lung adenocarcinoma transcript 1 (MALAT1) has been determined to play an important role in the modulation of oxidative stress and the development of cardiovascular diseases. Nevertheless, the function and underlying mechanism of MALAT1 in restenosis induced by hypertensive angioplasty remain unclear. AngII increased the expression of MALAT1 in VSMCs. We found that antisense oligonucleotide lncRNA MALAT1 (ASO-MALAT1) could inhibit AngII-induced reactive oxygen species production and VSMCs proliferation and migration by inducing the expression of glutathione peroxidase 4 (GPX4), which can be reversed by siRNA-GPX4. GPX4 overexpression can inhibit the proliferation and migration of VSMCs induced by AngII. In addition, we found that the process by which MALAT1 knockdown induces GPX4 expression involves nuclear factor erythrocyte 2–related factor 2 (Nrf2). Overexpression of Nrf2 can increase the expression of GPX4, and downregulation of GPX4 by ML385 (Nrf2 inhibitor) blocked the protective effect of ASO-MALAT1 on AngII-induced proliferation and migration of VSMCs. Ferrostatin-1 (Fer-1, ip 5 mg/kg per day for 2 weeks), a GPX4 agonist, significantly inhibited neointimal formation in spontaneously hypertensive rat by the inhibition of oxidative stress. In conclusion, these data imply that ASO-MALAT1 suppresses the AngII-induced oxidative stress, proliferation, and migration of VSMCs by activating Nrf2/GPX4 antioxidant signaling. GPX4 may be a potential target for the therapeutic intervention of hypertensive vascular restenosis.