bluuebunny/crossref_metadata_2025_split huggingface dataset:
UJI KANDUNGAN TANIN DAUN SALAM (SIZYGIUM POLYANTHUM) MENGGUNAKAN BERBAGAI KONSENTRASI ETANOL
Authors: Soraya Soraya 
Published: December 2023

Ekstrak daun salam mengandung alkaloid, flavonoid, saponin, steroid, terpenoid, minyak atsiri , sitral, dan eugenol, tanin. Tanin diketahui merupakan senyawa aktif metabolit sekunder yang memiliki berbagai manfaat dalam pengobatan dan industri makanan, karena memiliki sifat antioksidan, antibakteri, anti diare dan antitumor. Sampel daun salam yang digunakan dalam penelitian ini berasal dari daerah Banjarnaru, Kalimantan Selatan, Indonesia. Penelitian ini dilakukan dalam beberapa tahap, yaitu pengolahan bahan baku, pembuatan ekstrak, pemisahan ekstrak dan uji kadar tanin. Analisa produk menggunakan metode Spektrofotometri UV-Vis dengan larutan K₄Fe₆ dan FeCl₃ dan indikator asam galat. Dengan konsentrasi etanol 96% didapatkan kandungan tanin tertinggi yaitu 0,854 mg/mL dan kandungan kadar tanin terkecil pada konsentrasi 60% yaitu 0,188 mg/mL.
Duration of mechanical ventilation and clinical outcomes in very low birth weight infants: A single center 10-years cohort study
Authors: Menshykova A.O. , Dobryanskyy D.O. 
Published: December 2023

BACKGROUND: Despite the important role of MV in reducing mortality in very preterm infants, its use is often associated with complications. The study was aimed to determine the duration of mechanical ventilation (MV), which significantly increased the risk of adverse outcomes in very low birth weight (VLBW) infants. METHODS: Data obtained from a prospectively created computer database were used in a retrospective cohort study. The database included information about 1980 VLBW infants <32 weeks of gestation who were cared for at the tertiary care center between January 2010 and December 2020. RESULTS: Out of 1980 VLBW infants, 1086 (55%) were ventilated sometime during the hospital stay. 678 (62.43%) of ventilated babies survived until discharge. With ROC analysis, it was identified that MV duration of 60.5 hours had 79.3% sensitivity and 64.6% specificity for the prediction of BPD with the AUC of 0.784 (95% CI 0.733–0.827; p < 0.0001). The duration of MV above 60.5 hours was a significant risk factor for bronchopulmonary dysplasia (aOR 6.005, 95% CI 3.626–9.946), death (aOR 3.610, 95% CI 2.470–5.276), bronchopulmonary dysplasia/death (aOR 4.561, 95% CI 3.328–6.252), sepsis (aOR 1.634, 95% CI 1.168–2.286), necrotizing enterocolitis (aOR 2.606, 95% CI 1.364–4.980), and periventricular leukomalacia (aOR 2.191, 95% CI 1.241–3.867). CONCLUSIONS: Duration of MV longer than 60.5 hours is an independent risk factor for adverse outcomes in VLBW infants. It is essential to increase and optimize efforts to avoid MV or extubate very preterm infants as soon as possible, before reaching the established threshold duration of invasive respiratory support.
Postpartum care: Discussions and counseling for the peripartum period
Authors: Prince A. , Wade J. , Power M.L. , Gunawansa N. , Cruz-Bendezú A. , Schulkin J. , Macri C.J. 
Published: December 2023

BACKGROUND: Traditionally, postpartum care is confined to inpatient care immediately post birth and one appointment approximately six weeks postpartum. Data supports a continuum of care model as best for the health of mother and baby. Despite most women having significant concerns about the postpartum period, these concerns are frequently incompletely addressed by providers. We surveyed prenatal and postpartum patients to understand their concerns and experiences discussing postpartum care with providers. METHODS: Cross sectional surveys were administered between June 2019 and May 2021. Principal component analysis was used to show higher than average (positive) or lower than average (negative) conversations with providers about postpartum care examined by race, education, and parity. Chi squared tests were conducted to examine the significance of specific postpartum concerns. RESULTS: 421/450 patient surveys were analyzed, based on completion. Most patients were White (193), had post graduate degrees (188), privately insured (236), married (248), first time pregnant (152), and used doctors as their primary provider (267). Patients with lower education, higher parity and Black patients without postgraduate degrees reported higher than average postpartum counseling. Additionally, most patients expressed significant concerns about postpartum exhaustion (65.8%), breastfeeding (62.3%), pain (61.2%), physical activity (54.9%) and the baby blues (50.4%). CONCLUSIONS: Postpartum concerns are incompletely and inconsistently addressed amongst patients based on race, parity, and education. A continuum of care approach, beginning in the third trimester, through the postpartum period, may provide better counseling to address all patients’ concerns.
Health literacy: A missing link to breastfeeding protection, promotion and support?
Authors: Abbass-Dick Jennifer , McQueen Karen , Lemonde Manon , Dubrowski Adam , Dennis Cindy-Lee 
Published: December 2023

Breastfeeding is a public health priority, recommended by all leading health authorities. Although numerous interventions and global initiatives have demonstrated some effect on improving breastfeeding outcomes, breastfeeding rates remain suboptimal. Educational support is a component of these interventions that assists families to establish and maintain breastfeeding. Although parents are being provided with education, information that is received appears to be inconsistent resulting in breastfeeding recommendations not being met. The objectives of this paper are to discuss health literacy, the evidence in support of health literacy, and potential strategies to guide the implementation of breastfeeding interventions and program development.
Pengaruh Kepemimpinan Kepala Sekolah Dan Budaya Organisasi Terhadap Kinerja Guru
Authors: Noorsanti Noorsanti , Zamroni Zamroni , Salehudin Mohammad 
Published: December 2023

Tujuan penelitian ini untuk mengetai pengaruh kepemimpinan kepala sekolah dan budaya organisasi terhadap kinerja guru Di SD Kreatif Muhammadiyah 2 Bontang sesuai dengan hipotesis penelitian 1). Terdapat pengaruh yang signifikan dari kepemimpinan kepala sekolah (X1) terhadap kinerja guru (Y). 2). Terdapat pengaruh yang signifikan dari budaya organisasi (X2) terhadap kinerja guru (Y) di SD Kreatif Muhammadiyah 2 Bontang. Dan 3). Terdapat pengaruh antara kepemimpinan (X1) dan budaya organisai (X2) secara bersama-sama terhadap kinerja guru (Y) di SD Kreatif Muhammadiyah 2 Bontang. Penelitian kuantitatif ini menggunakan analisis regresi dengan menguji hipotesis menggunakan analisis uji t dan F, responden merupakan guru SD Kreatif Muhammadiyah 2 Bontang yang berjumlah 33 orang. Sedangkan instrumen telah dilakukan uji validitas dan reliabelitas menggunakan SPSS. Hasil penelitian menemukan, Uji Hipotesis 1 dibuktikan dari hasil uji t menunjukkan angka 0,005 < 0,05 dengan analisis nilai t tabel lebih kecil dibandingkan dengan t hitung. Ada pengaruh dari kepemimpinan (X1) memiliki pengaruh yang positif terhadap kinerja guru (Y). Maka Ho positif diterima. Hasil uji hipotesis 2 dibuktikan dari hasil uji t menunjukkan angka 0,000< 0,05 dengan analisis nilai t tabel lebih kecil dibandingkan dengan t hitung. Ada pengaruh yang positif dari Budaya Organisasi (X2) terhadap Kinerja Guru . Maka Ho positif diterima. Untuk uji hipotesis 3 ditemukan dibuktikan dari hasil uji F menunjukkan angka 0,000< 0,05 dengan analisis nilai t tabel lebih kecil dibandingkan dengan t hitung Berdasarkan pengaruh secara parsial yang terjadi antara variabel bebas (kepemimpinan dan budaya organisasi) terhadap variable terikat ( kinerja guru ), hasil yang diperoleh nilai signifikansi budaya organisasi sebesar 0,000 < 0,05 maka Hipotesis diterima, nilai signifikansi kepemimpinan kepala sekolah 0,005 < dari 0,05 maka Hipotesis diterima dan nilai signifikan budaya organisasi dan kepemimpinan kepala sekolah secara simultan 0,000 < 0,005 maka Hipotesis diterima ditunjukkan nilai R square sebesar 89%.
Efektivitas vectobac dan predator Mesocyclops aspericornis sebagai jasad pengendali hayati jentik Aedes aegypti dalam gentong air
Authors: Ambar Yuniarti Retno , Ch.P Blondine 
Published: December 2023

Dengue Haemorrhagic Fever (DHF) was one of the acute and infectious disease and was still a problem of public health in Indonesia. This was caused the spread of the Aedes aegypti mosquito in the country, except at the altitude of more than one thousand meters above sea level. The spread of DHF was connected with the breeding place of the DHF vector. The use of chemical insecticides for vector control has been conducted for some time. Frequent use of insecticides has caused resistence of the mosquito towords the insecticide. Nowdays, biological control agents was developed. The aim of the study is to know the effectivity of the VectoBac and Mesocyclops aspericornis predator both applied individually and combination for controling of Ae. aegypti larvae in the water jar. The study was conducted in January until August 2003 in a DHF endemic area at Kupang Rengas, Kupang Village, Ambarawa subdistrict, Semarang regency, Central Java. The design of the study was quasy experimental comparing before and after intervention using external difference groups. Purposive sampling was conducted. The ten sample size were used for each treatment. The result showed that VectoBac was effective to reduce the Ae. aegypti larvae density until the fifth week reaching a percentage of 79,31 – 96,04%. M. aspericornis predator was effective to reduce the Ae. aegypti larvae density in the seventh week until the twelfth week was 70,69 – 75,09%. The combination of VectoBac and M. aspericornis predator was effective to reduce Ae. aegypti larvae density until the twelfth week reaching a percentage of 96,56 – 100%.
Facilitating Intercultural Communicative Competence through a Multiliteracies approach: musical input as the missing link
Authors: Fernández-García Antonio 
Published: December 2023

Foreign Language (FL) learning has undergone a shift away from the communicative approach and towards a pedagogy of Multiliteracies. In an ever-changing society characterized by a digital and multicultural context, the necessity of developing Intercultural Communicative Competence (ICC) in FL learning across all levels through a pedagogy of Multiliteracies has become a priority. In this regard, musical input is an ideal medium to explore culture in the FL learning classroom which enables the integration of affective experiences from an intercultural perspective. The reasons for the implementation of musical input into FL learning curricula to develop ICC is twofold: on the one hand, it may serve to stimulate students’ awareness towards the target culture; and, on the other hand, it may be an effective way to engage students in intercultural encounters from an affective point of view. A corpus has been designed in order to analyze those studies on the development of ICC in FL learning across all levels through a Multiliteracies approach. The findings demonstrate that the use of musical input to develop ICC in the FL learning classroom through a pedagogy of Multiliteracies is scarce. Moreover, this study seeks to promote musical input-based activities to facilitate the development of ICC in the FL learning classroom through a Multiliteracies pedagogy.
Co-production of pigment and high value-added bacterial nanocellulose from Suaeda salsa biomass with improved efficiency of enzymatic saccharification and fermentation
Authors: Tan Ran , Sun Qiwei , Yan Yiran , Chen Tao , Wang Yifei , Li Jiakun , Guo Xiaohong , Fan Zuoqing , Zhang Yao , Chen Linxu , Wu Guochao , Wu Nan 
Published: December 2023

This study evaluated the co-production of pigment and bacterial nanocellulose (BNC) from S. salsa biomass. The extraction of the beet red pigment reduced the salts and flavonoids contents by 82.7%–100%, promoting the efficiencies of enzymatic saccharification of the biomass and the fermentation of BNC from the hydrolysate. SEM analysis revealed that the extraction process disrupted the lignocellulosic fiber structure, and the chemical analysis revealed the lessened cellulase inhibitors, consequently facilitating enzymatic saccharification for 10.4 times. BNC producing strains were found to be hyper-sensitive to NaCl stress, produced up to 400.4% more BNC from the hydrolysate after the extraction. The fermentation results of BNC indicated that the LDU-A strain yielded 2.116 g/L and 0.539 g/L in ES-M and NES-M, respectively. In comparison to the control, the yield in ES-M increased by approximately 20.0%, while the enhancement in NES-M was more significant, reaching 292.6%. After conducting a comprehensive characterization of BNC derived from S. salsa through Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Thermogravimetric Analysis (TGA), the average fiber diameter distribution of these four BNC materials ranges from 22.23 to 33.03 nanometers, with a crystallinity range of 77%–90%. Additionally, they exhibit a consistent trend during the thermal degradation process, further emphasizing their stability in high-temperature environments and similar thermal properties. Our study found an efficient co-production approach of pigment and BNC from S. salsa biomass. Pigment extraction made biomass more physically and chemically digestible to cellulase, and significantly improved BNC productivity and quality.
Analysis of clinical characteristics and imagological features of the aortic dissection patients with negative D-dimer results
Authors: Zhang Zhixiang , Wang Lilan , Su Xin , Zhou Yuling , Wu Kaimin , Sun Guangfeng , Ou Weimei , Yu Lihong , Chen Weifen , Wang Bin 
Published: December 2023

BackgroundD-dimer (DD) is a vital biomarker to rule out the diagnosis of aortic dissection (AD). However, the DD level in some patients with AD is not high in clinical practice, which often leads to missed diagnosis; therefore, understanding the characteristics of patients with AD and negative DD is of great clinical value.MethodsFrom May 2015 to October 2020, 286 patients with AD who visited the first medical contact (FMC) within 24 h of symptom onset and were hospitalized in the Xiamen Cardiovascular Hospital of Xiamen University were enrolled in this study. Clinical characteristics and outcomes of patients were assessed.ResultsAmong them, 13 cases (approximately 4.5%) had negative DD results. Compared to patients with positive DD results, patients with negative DD results had significantly higher platelet counts and lower aortic dissection detection risk scores (ADD-RS). The imagological analysis showed that patients with AD and negative DD had lower extension scores and milder damage to the mesenteric artery and three branches of the aortic arch. Furthermore, the results of the multivariable analysis showed that white blood cell count (WBC) [odds ratio (OR): 1.379, P = 0.028], FMC (OR: 0.904, P = 0.028), and extension score (OR: 1.623, P = 0.046) were associated with negative DD result.ConclusionsPatients with AD and negative DD results had longer FMC and lower WBC. Imaging showed a smaller tear extension range and less damage to the mesenteric artery and three branches of the aortic arch. A negative DD result could not completely rule out AD even if the ADD-RS was zero.
Whole genome sequencing in the palm of your hand: how to implement a MinION Galaxy-based workflow in a food safety laboratory for rapid Salmonella spp. serotyping, virulence, and antimicrobial resistance gene identification
Authors: Lamas Alexandre , Garrido-Maestu Alejandro , Prieto Alberto , Cepeda Alberto , Franco Carlos Manuel 
Published: December 2023

IntroductionWhole Genome Sequencing (WGS) implementation in food safety laboratories is a significant advancement in food pathogen control and outbreak tracking. However, the initial investment for acquiring next-generation sequencing platforms and the need for bioinformatic skills represented an obstacle for the widespread use of WGS. Long-reading technologies, such as the one developed by Oxford Nanopore Technologies, can be easily implemented with a minor initial investment and with simple protocols that can be performed with basic laboratory equipment.MethodsHerein, we report a simple MinION Galaxy-based workflow with analysis parameters that allow its implementation in food safety laboratories with limited computer resources and without previous knowledge in bioinformatics for rapid Salmonella serotyping, virulence, and identification of antimicrobial resistance genes. For that purpose, the single use Flongle flow cells, along with the MinION Mk1B for WGS, and the community-driven web-based analysis platform Galaxy for bioinformatic analysis was used. Three strains belonging to three different serotypes, monophasic S. Typhimurium, S. Grancanaria, and S. Senftenberg, were sequenced.ResultsAfter 24 h of sequencing, enough coverage was achieved in order to perform de novo assembly in all three strains. After evaluating different tools, Flye de novo assemblies with medaka polishing were shown to be optimal for in silico Salmonella spp. serotyping with SISRT tool followed by antimicrobial and virulence gene identification with ABRicate.DiscussionThe implementation of the present workflow in food safety laboratories with limited computer resources allows a rapid characterization of Salmonella spp. isolates.
Salivary proteomic profile of young healthy subjects
Authors: Dongiovanni P. , Meroni M. , Aiello Gilda , D’Amato A. , Cenzato N. , Casati S. , Damiani G. , Fenoglio C. , Galimberti D. , Grossi E. , Prati D. , Lamorte G. , Bianco C. , Valenti L. , Soggiu A. , Zapperi S. , La Porta C. A. M. , Del Fabbro M. , Tartaglia G. M. 
Published: December 2023

Background: The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedures of collection/analysis and the lack of normal ranges makes the use of saliva still tricky, our purpose was to outline a salivary proteomic profile which features healthy individuals.Methods: We collected saliva samples from 19 young blood donors as reference population and the proteomic profile was investigated through mass-spectrometry.Results: We identified 1,004 proteins of whose 243 proteins were shared by all subjects. By applying a data clustering approach, we found a set of six most representative proteins across all subjects including Coronin-1A, F-actin-capping protein subunit alpha, Immunoglobulin J chain, Prosaposin, 78 kDa glucose-regulated protein and Heat shock 70 kDa protein 1A and 1B.Conclusion: All of these proteins are involved in immune system activation, cellular stress responses, proliferation, and invasion thus suggesting their use as biomarkers in patients with NCDs.
Hypermethylated CDO1 and CELF4 in cytological specimens as triage strategy biomarkers in endometrial malignant lesions
Authors: Qi Bingli , Sun Ye , Lv Yaohua , Hu Pei , Ma Yanli , Gao Wenying , Li Shumei , Zhang Xin , Jin Xitong , Liou Yuligh , Liu Pei , Liu Shikai 
Published: December 2023

ObjectiveDeveloping a non-invasive and reliable triage test for endometrial malignant lesions is an important goal, as it could help to reduce the number of invasive diagnostic procedures required and improve patient survival. We aimed to estimate the diagnostic value of DNA methylation levels in cervical cytological samples of endometrial cancer (EC) and endometrial atypical hyperplasia (AH).MethodsA total of 607 women who had indications for endometrial biopsy in the Department of Obstetrics and Gynecology of Cangzhou Central Hospital from October 2022 to April 2023 were enrolled in this study. The cervical exfoliated cells were collected for gene methylation before endometrial biopsy. Clinical information, tumor biomarkers, and endometrial thickness (ET) of transvaginal ultrasonography (TVS) were also collected. With endometrial histopathology as the gold standard, multivariate unconditional logistic regression was applied to analyze the risk factors of endometrial malignant lesions. The role of cysteine dioxygenase type 1 (CDO1) and CUGBP Elav-like family member 4 (CELF4) gene methylation as a triage strategy biomarker in endometrial malignant lesions was specifically explored.ResultsMultivariate logistic regression analysis showed that premenopausal ET ≥ 11 mm or postmenopausal ET ≥ 5 mm, CDO1 ΔCt ≤ 8.4, or CELF4 ΔCt ≤ 8.8 were the risk factors for AH and EC, with odds ratios (ORs) (95%CI) of 5.03 (1.83–13.82) and 6.92 (1.10–43.44), respectively (p-values &lt; 0.05). The sensitivity and specificity of CDO1/CELF4 dual-gene methylation assay for AH and EC reached 84.9% (95%CI: 75.3%–94.5%) and 86.6% (95%CI: 83.8%–89.5%), respectively. ET combined with DNA methylation detection further improved the specificity to (94.9%, 95%CI: 93.1%–96.8%).ConclusionThe accuracy of cervical cytology DNA methylation is superior to that of other clinical indicators in the non-invasive examination of endometrial malignant lesions. DNA methylation combined with TVS can further improve the specificity and is a promising biomarker triage strategy in women with suspected endometrial lesions.
Is apomixis occurring in walnut (Juglans regia L.)? New data from progeny molecular tests and cytological investigations shed light on its reproductive system
Authors: Solhjoo Sahar , Fatahi Reza , Zamani Zabihollah , Chehregani Rad Abdolkarim , Palumbo Fabio , Barcaccia Gianni 
Published: December 2023

IntroductionPersian walnut (Juglans regia) is an economically important nut fruit species cultivated worldwide for its nutritious kernel and timber quality wood. Walnut trees are mostly hetero-dichogamous and, depending on the genotype, some cultivars are protogynous, while others are protandrous. Although selfing is possible when male and female blooms overlap, the dichogamy of the species promotes outcrossing. In addition to sexual reproduction, some reports indicate that elements of apomixis may occur in commercial orchards of walnut varieties and in the last two decades, nut production by apomixis has been reported in walnut. However, there are no reliable studies on the occurrence of apomictic reproduction based on cytoembryological observations and/or molecular marker-progeny tests. This study addresses the combined use of molecular and cytological analyses to gain new insights into the population genetics and reproduction systems of J. regia.MethodsWe systematically analyzed the reproductive origin of individual progeny plants from 8 different cultivated walnut genotypes using microsatellite genotyping and carried out cytohistological investigations of 5 cultivated walnut genotypes arising seed sets from isolated flowers, to shed light on the mode of reproduction.Results and discussionThese cytometric and genotyping analyses did not support any asexual mode of reproduction or asexual propagation by seed and all individuals studied were identified as zygotic plants produced by crossing. Likewise, the cytological findings did not confirm completely the first component of apomixis, namely apomeiosis. On the other hand, according to histological evidence, adventitious embryony seems to take place at low frequency. Overall, our findings suggest that the occurrence of gametophytic apomixis is unlikely in J. regia, but sporophytic apomixis cannot be completely ruled out.