bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbapenemase producing Enterobacterales clinical isolates from a tertiary care hospital in Egypt
Authors: El Defrawy Inas , Salem Dalia , Ali Ghada , Gamal Doaa , El Dabaa Ehab , Diab Manal , Alyan Sohair Abd-Elmaguid , Sallam Marwa K. 
Published: November 2023

Abstract
Background
Carbapenem resistant Enterobacterales (CRE) is on the rise globally, triggering a significant health threat and a substantial concern for infection control management. We aimed to detect and characterize carbapenemases producing Enterobacterales (CPE) clinical isolates over a period of nearly one-year duration in Theodor Bilharz Research Institute, a tertiary care hospital in Egypt through molecular and phenotypic methods using carbapenemase detection combination inhibitor disk set (Enterobacterales) MASTDISCS ID (MDI) (MAST, UK), with the addition of temocillin disk.

Results
CRE represented 6.5% of Enterobacterales. Healthcare-associated infections were frequently high representing 87% of the CRE isolated from hospitalized patients. Most of the CRE isolates were Klebsiella pneumonia (68%) followed by Escherichia coli (22%), Enterobacter cloacae (4%), Serratia marcescens (4%) and Citrobacter freundii (2%). Phenotypic detection revealed metallo-β lactamases in 84% of isolates, followed by oxacillinase-48 {(OXA-48) 6%} and Klebsiella pneumoniae carbapenemase in 2% of the isolates. The most prevalent gene detected by conventional PCR was blaNDM (84%) followed by blaOXA-48 (6%) and blaKPC (2%). Excellent agreement was found between PCR and MDI for detection of carbapenemase production.

Conclusions
NDM carbapenemase is prevalent in our hospital. Carbapenemase detection combination inhibitor disk set (Enterobacterales) MASTDISCS ID is a useful tool for rapid and precise confirmation of the detection of CPE.
Modified nondominated sorting genetic algorithm-based multiobjective optimization of a cross-coupled nonlinear PID controller for a Twin Rotor System
Authors: Sivadasan J. , Shiney J. Roscia Jeya 
Published: November 2023

AbstractThis paper presents an innovative nonlinear PID control scheme based on a modified nondominated sorting genetic algorithm validated using a laboratory helicopter model called the Twin Rotor System. The concepts of controlled elitism and dynamic crowding distance are incorporated into the proposed algorithm to progress towards the best solution from the entire population in order to solve the multiobjective optimization problem with good convergence characteristics. The addition of nonlinear functions to the cross-coupled PID controller structure initiates better error tracking and facilitates smooth output under changing input conditions. The design objective is to implement an optimal nonlinear PID control scheme for the angular displacements of the twin-rotor system, with integral square error and control energy taken as the multiobjective problems. The statistical performance of the controller is analyzed by considering the best, worst, mean, and standard deviations of ISE. In this work, simultaneous control of pitch and yaw angles is considered to get rid of the coupling effect between the two rotors. The results indicate the advantage of the MNGSA-based tuning for the two degrees of freedom MIMO control with standard reference trajectories as per the TRMS330-10 model.
Migration background, eating disorder symptoms and healthcare service utilisation: findings from the Stockholm Public Health Cohort
Authors: Strand Mattias , Bäärnhielm Sofie , Fredlund Peeter , Brynedal Boel , Welch Elisabeth 
Published: November 2023

BackgroundFrom a global perspective, eating disorders are increasingly common, probably because of societal transformation and improved detection. However, research on the impact of migration on the development of eating disorders is scarce, and previously reported results are conflicting.AimsTo explore if eating disorder symptom prevalence varies according to birth region, parents’ birth region and neighbourhood characteristics, and analyse if the observed patterns match the likelihood of being in specialist treatment.MethodThis study uses data from a large population-based health survey (N=47 662) among adults in Stockholm, Sweden. A general linear model for complex samples, including adjustment for gender and age, was used to explore self-reported eating disorder symptoms. Odds ratios were calculated for individual symptoms.ResultsEating disorder symptoms are substantially more common in individuals born abroad, especially for migrants from a non-European country. This holds true for all surveyed symptoms, including restrictive eating (odds ratio 5.5, 95% CI 4.5–6.7), compensatory vomiting (odds ratio 6.1, 95% CI 4.6–8.0), loss-of-control eating (odds ratio 2.6, 95% CI 2.3–3.1) and preoccupation with food (odds ratio 2.3, 95% CI 1.9–2.8). Likewise, symptoms are more common in individuals with both parents born abroad and individuals living in districts with a high percentage of migrant residents. A gap exists between district-level symptom scores and the likelihood of being in specialist eating disorder treatment.ConclusionsThese findings call for oversight of current outreach strategies, and highlight the need for efforts to reduce stigma and increase eating disorder symptom recognition in broader groups.
Pluripotent stem cell-derived neural progenitor cells can be used to model effects of IL-6 on human neurodevelopment
Authors: Sarieva Kseniia , Hildebrand Felix , Kagermeier Theresa , Yentür Zeynep , Becker Katharina , Mayer Simone 
Published: November 2023

ABSTRACT
Maternal immune activation (MIA) increases the risks for neurodevelopmental disorders in offspring through inflammatory cytokines, including interleukin-6 (IL-6). We therefore aimed to establish a human two-dimensional (2D) in vitro neural model to investigate the effects of IL-6 exposure on neurodevelopment. IL-6 signal transduction requires two receptors: interleukin-6 signal transducer (IL6ST) and interleukin-6 receptor (IL6R). Prenatally, neural cells lack IL6R, and hence cannot elicit cis IL-6 signaling, but IL6R can be provided by microglia in trans. We demonstrate here that an immortalized human neural progenitor cell (NPC) line, ReNCell CX, expresses IL6ST and elicits both cis and trans IL-6 signaling, limiting its use as a model of MIA. In contrast, induced pluripotent stem cell (iPSC)-derived NPCs only activate the IL-6 cascade in trans. Activation of the trans IL-6 cascade did not result in increased proliferation of iPSC-derived NPCs or ReNCell CX, as has been demonstrated in animal models. iPSC-derived NPCs upregulated NR2F1 expression in response to IL-6 signaling in line with analogous experiments in organoids. Thus, iPSC-derived NPCs can be used to model gene expression changes in response to MIA in 2D cultures.
Multiple axes of visual system diversity in Ithomiini, an ecologically diverse tribe of mimetic butterflies
Authors: Wainwright J. Benito , Schofield Corin , Conway Max , Phillips Daniel , Martin-Silverstone Elizabeth , Brodrick Emelie A. , Cicconardi Francesco , How Martin J. , Roberts Nicholas W. , Montgomery Stephen H. 
Published: November 2023

ABSTRACT
The striking structural variation seen in arthropod visual systems can be explained by the overall quantity and spatio-temporal structure of light within habitats coupled with developmental and physiological constraints. However, little is currently known about how fine-scale variation in visual structures arises across shorter evolutionary and ecological scales. In this study, we characterise patterns of interspecific (between species), intraspecific (between sexes) and intraindividual (between eye regions) variation in the visual system of four ithomiine butterfly species. These species are part of a diverse 26-million-year-old Neotropical radiation where changes in mimetic colouration are associated with fine-scale shifts in ecology, such as microhabitat preference. Using a combination of selection analyses on visual opsin sequences, in vivo ophthalmoscopy, micro-computed tomography (micro-CT), immunohistochemistry, confocal microscopy and neural tracing, we quantify and describe physiological, anatomical and molecular traits involved in visual processing. Using these data, we provide evidence of substantial variation within the visual systems of Ithomiini, including: (i) relaxed selection on visual opsins, perhaps mediated by habitat preference, (ii) interspecific shifts in visual system physiology and anatomy, and (iii) extensive sexual dimorphism, including the complete absence of a butterfly-specific optic neuropil in the males of some species. We conclude that considerable visual system variation can exist within diverse insect radiations, hinting at the evolutionary lability of these systems to rapidly develop specialisations to distinct visual ecologies, with selection acting at the perceptual, processing and molecular level.
Digital holography without a dark room environment: extraction of interference fringes by using deep learning
Authors: Nagahama Yuki 
Published: November 2023

When obtaining digital holograms, dark rooms are used to prevent the influence of natural light on the formation of holograms. Further, in recent years, researchers have actively studied machine learning techniques such as deep learning to resolve image-related problems. In this study, we obtained a pair of holograms influenced by natural light and holograms unaffected by natural light, and trained U-Net to perform image transformation to remove the effects of natural light from holograms. Thus, this study aimed to propose a method for eliminating the effects of natural light from holograms by using the U-Net we trained. To verify the effectiveness of the proposed method, we evaluated the image quality of the reconstructed image of holograms before and after image processing by U-Net. The results showed that the peak signal-to-noise ratio (PSNR) increased by 7.38 [dB] after processing by U-Net. Additionally, the structural similarity index (SSIM) increased by 0.0453 after processing by U-Net. This study confirmed that in digital holography, holograms can be acquired without the use of a dark room and that the method proposed in this study can eliminate the effects of natural light and produce high-quality reconstructed images.
Factors important for health-related quality of life in men and women: The population based SCAPIS study
Authors: Olsson Max , Björkelund Anders J. , Sandberg Jacob , Blomberg Anders , Börjesson Mats , Currow David , Malinovschi Andrei , Sköld Magnus , Wollmer Per , Torén Kjell , Östgren Carl-Johan , Engström Gunnar , Ekström Magnus 
Published: November 2023

Introduction
Health-related quality of life (HRQoL) is essential for human wellbeing, influenced by a complex interplay of factors, and is reported lower in women than men. We aimed to evaluate which factors were the most important for HRQoL in a middle-aged general population.

Methods
This was a cross-sectional, multi-centre study of 29,212 men (48%) and women (52%) aged 50–64 in the general population in Sweden. Physical and mental HRQoL (0–100) was assessed using the Short Form 12 questionnaire, and association was evaluated for 356 variables including demographics, lifestyle, symptoms, physiological measurements, and health conditions. Using machine learning, each variable´s importance for HRQoL was measured by an importance score, comparable to effect size, and summarised in 54 factors, in men and women separately.

Results
Men and women had similar mean and standard deviation (SD) scores for physical HRQoL (53.4 [SD 8.1] vs 51.4 [9.7]) and mental HRQoL (37.1 [5.0] vs 37.3 [5.4]). The most important factors for physical HRQoL were (importance score) physical activity (40), employment (36), pain (33), sleep (33), and sense of control (26). The most important factors for mental HRQoL were sense of control (18), physical activity (12), depression (12), pain (6), and employment (5).

Conclusions
The factors important for HRQoL identified by this study are likely to be amenable to interventions, and our findings can support prioritising interventions. The identified factors need to be a target even before middle-age to lay the foundation for long and happy lives.
Absence of the Nonextension Sign as a Marker for Endoscopic Submucosal Dissection Suitability
Authors: Carvajal González Aldo , Sandoval Montero Jorge , Alvarado Salazar Marianela , Chavarría Soto Ivannia , Chavarría Picado Alexa 
Published: November 2023

ABSTRACT
Early gastric cancer is defined as gastric cancer confined to the mucosa or submucosa, irrespective of lymph node metastasis. Endoscopic submucosal dissection is a standard treatment of early gastric cancer. The nonextension sign consists of a protrusion of the surrounding mucosa into the lumen, resembling a trapezoidal shape. This phenomenon occurs when massive submucosal invasion by a cancer increases the thickness and rigidity of the gastric wall and may be useful to differentiate between mucosal or microinvasive submucosal and invasive submucosal cancers. Our case demonstrates the usefulness of the absence of the conventional endoscopic nonextension sign as a marker to decide the adequate approach for mucosal lesions.
Color tunable benzothiadiazole-based small molecules for lightening applications
Authors: Kaya Nusret 
Published: November 2023

Abstract
2,1,3-Benzothiadiazole (BTD) is a key motif for the chemistry of photoluminescence molecules and its application in lightening technology as well as cell imagining. Evaluating the characteristics and reaction mechanisms of the BTD derivatives in molecular is critical for molecular designing. Research has been turned to BTD-extended derivatives owing to their possible usage in organic photovoltaics, organic light-emitting diodes, liquid crystal devices, dye-sensitized solar cells, and many other applications. Herein, three small molecules with a BTD core, 4,7-di-m-tolylbenzo[c][1,2,5]thiadiazole (NK1), 4,7-bis(3,4,5-trifluorophenyl)benzo[c][1,2,5]thiadiazole (NK2), and 4,7-bis(5-(m-tolyl)thiophen-2-yl)benzo[c][1,2,5]thiadiazole (NK3), were designed and synthesized and their molecular structures were elucidated. Additionally, their photophysical and electrochemical behaviors as well as the aggregation-induced enhanced emission properties were examined to investigate their possible usage in cell imaging and organic electronic applications. The HOMO energy level of molecules has been found as −5.96, −5.58, and −5.60 eV, respectively. In the emission spectra, it was seen that molecules showed green, blue, and red emission, respectively.
Bayesian and E-Bayesian estimation based on constant-stress partially accelerated life testing for inverted Topp–Leone distribution
Authors: Al Mutairi Aned , Alrashidi Afaf , Al-Sayed Neama Taher , Behairy Sarah Mohammad , Elgarhy Mohammed , Nassr Said G. 
Published: November 2023

Abstract
Accelerated or partially accelerated life tests are particularly significant in life testing experiments since they save time and cost. Partially accelerated life tests are carried out when the data from accelerated life testing cannot be extrapolated to usual conditions. The constant-stress partially accelerated life test is proposed in this study based on a Type-II censoring scheme and supposing that the lifetimes of units at usual conditions follow the inverted Topp–Leone distribution. The Bayes and E-Bayes estimators of the distribution parameter and the acceleration factor are derived. The balanced squared error loss function, which is a symmetric loss function, and the balanced linear exponential loss function, which is an asymmetric loss function, are considered for obtaining the Bayes and E-Bayes estimators. Based on informative gamma priors and uniform hyper-prior distributions, the estimators are obtained. Finally, the performance of the proposed Bayes and E-Bayes estimates is evaluated through a simulation study and an application using real datasets.
Structural insights into coordinating 5S RNP rotation with ITS2 pre‐RNA processing during ribosome formation
Authors: Thoms Matthias , Lau Benjamin , Cheng Jingdong , Fromm Lisa , Denk Timo , Kellner Nikola , Flemming Dirk , Fischer Paulina , Falquet Laurent , Berninghausen Otto , Beckmann Roland , Hurt Ed 
Published: November 2023

AbstractThe rixosome defined in Schizosaccharomyces pombe and humans performs diverse roles in pre‐ribosomal RNA processing and gene silencing. Here, we isolate and describe the conserved rixosome from Chaetomium thermophilum, which consists of two sub‐modules, the sphere‐like Rix1‐Ipi3‐Ipi1 and the butterfly‐like Las1‐Grc3 complex, connected by a flexible linker. The Rix1 complex of the rixosome utilizes Sda1 as landing platform on nucleoplasmic pre‐60S particles to wedge between the 5S rRNA tip and L1‐stalk, thereby facilitating the 180° rotation of the immature 5S RNP towards its mature conformation. Upon rixosome positioning, the other sub‐module with Las1 endonuclease and Grc3 polynucleotide‐kinase can reach a strategic position at the pre‐60S foot to cleave and 5′ phosphorylate the nearby ITS2 pre‐rRNA. Finally, inward movement of the L1 stalk permits the flexible Nop53 N‐terminus with its AIM motif to become positioned at the base of the L1‐stalk to facilitate Mtr4 helicase‐exosome participation for completing ITS2 removal. Thus, the rixosome structure elucidates the coordination of two central ribosome biogenesis events, but its role in gene silencing may adapt similar strategies.
Place and Purpose of Vocal Ensembles in Liturgy
Authors: Szlachetka Tomasz 
Published: November 2023

Over the centuries, singing ensembles have occupied assorted liturgical locations in church space. Nowadays – pursuant to music-related church laws – they ought to occupy a place duly highlighting their special participation and purpose in liturgy. Singing group place[1]ment shall take account of its composition as well. The placement and purpose of signing ensembles alike have to be viewed in the context of Church traditions and laws. In terms of the nature and tasks assigned to specific singing groups in liturgy, they may be classified as the choir and schola cantorum, respectively. A synthetic presentation of the topic will be particularly helpful to persons responsible for preparing solemn liturgical celebrations
INVESTIGATION TORSION SYSTEMS OF BLANKS ROTATION IN INFEED GRINDING ON MACHINE-TOOLS MOD.3A151 AND 3A152
Authors: Sizy Jury , Stalinsky Dmitry , Yevsiukova Fatyma , Shcherbinina Tatiana , Ushakov Oleksiy 
Published: November 2023

In article presented investigation of static and dynamic characteristics torsion systems of blanks rotation in the machine-tools produced by Kharkov machine-tool plant. These blanks rotation systems differ in v-belt transmissions from rotor of electric motor to spindle of headstock.
In the machine-tool 3A151 this v-belt transmission is two-steps with total transmission relation 4.4 , but in the machine-tool 3M152 is one step with transmission relation 2.5. This investigation was realized on the base of system differential equations (DE) balance discrete systems by state space equations and with the help of structure rotation systems in the view transfer functions elements of drivers and the ties between thems.
In (DE) are take account damping energy of rotation vibrations in electromagnetic ties rotor with stator direct-current motor. From drivers models in state space was received its gain-frequency characteristics in input action on the drivers cutting moment with autput action by angular velocity of blank. The analysis of roots characteristic equations of transfer functions showed its stability but long damping with very little amplitude of vibrations, which cannot make worse of geometrical quality grinding surface. The drivers which compare are not different by
their dynamic characteristics. But they very different by their statistic characteristics. It was established that in driver with two-step belt transmission with motor 2PD100MYXL4 impossibly get rotary velocity of blanks little diameters (30-40)mm over 50m/min which demands for reduce of maximum temperatures heating of surface in infeed grinding. For driver with one step belt transmission this limitation not take place. It was established that energy of grinding in driver with two-step belt transmission in 1.5 times more of it in driver with two-step belt transmission. Such as changing driver in machine-tool 3M152 with comparison machine-tool 3M151 was purposely.
El aprendizaje basado en la resolución de problemas: una experiencia práctica
Authors: Recio Ramírez Mª Ángeles 
Published: November 2023

La acomodación de la docencia universitaria de la disciplina Derecho Financiero y Tributario a los nuevos parámetros del Espacio Europeo de Educación Superior (EEES), conocido como "proceso de Bolonia", ha hecho necesario activar metodologías y herramientas docentes que faciliten la implantación de la enseñanza centrada en el aprendizaje activo, autónomo y participativo. Entre la variedad de metodologías didácticas, se ha optado por el "Aprendizaje basado en la resolución de problemas", siendo el objetivo fundamental de nuestra experiencia, enfrentar al alumnado con casos prácticos que acontecen en el trabajo diario de un asesor fiscal. Los resultados fueron muy satisfactorios, debido a la gran implicación del grupo de alumnos, y a las calificaciones positivas obtenidas al final del proceso evaluador.The accommodation of de university teaching of the discipline Financial and Tax Law to the new parameters of the European Higher Education, known as Bologna process, it has become necessary to enable methodologies and teaching tools that facilitate the introduction of teaching active, autonomous, learning centered and participatory. Between the variety of teaching methodologies, has been chosen by problem based learning, being the main objective of our experience, confront the student with practical cases that occur in the daily work of a tax advisor. The results were very satisfactory because the great involvement of the group of students, and the positive qualifications obtained at the end of the evaluation process.
An appetitive spatial working memory task for mice, using a semi-automated 8-arm radial maze
Authors: Shimizu Takahiro , Nayar Stuart G , Richardson William D 
Published: November 2023

Abstract
This methods paper details a protocol to test spatial working memory in mice using a semi-automated radial 8-arm maze (RAM). The RAM is a partially enclosed apparatus with 8 horizontal equally spaced arms radiating from a central hub, from which access to each arm can be controlled individually by servo-controlled motorized doors. Animals start in the central hub and are allowed to explore the maze for a food reward at the end of each arm or selected arms. The RAM task was originally designed for rats but we have adapted the protocol for mice – for example, by including more habituation steps. In our protocol, all arms are initially baited with sweetened condensed milk and mice are admitted sequentially into four pseudo-randomly selected arms to collect the rewards (“forced run”) before opening all doors together to allow the mice to run freely and find the remaining four rewards (“free run”). A 5-sec time delay is introduced between the forced and free runs to challenge working memory; an error is recorded if the mouse enters any previously-visited arm during the free run. The task is complete when all rewards are recovered. After 6 days of habituation and 9 days of maze training, male C57BL/6 mice regularly achieve ≥80% success rate, defined as 4/(4+E) where E is the number of errors. This semi-automated task could in principle be combined with in vivo monitoring methods such as electrophysiology or calcium imaging.