bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of Caco-2 cells and human jejunal and duodenal enteroid-derived cells in gel- and membrane-based barrier models of intestinal permeability
Authors: Moyer Haley L , Vergara Leoncio , Stephan Clifford , Sakolish Courtney , Ford Lucie C , Tsai Han-Hsuan D , Lin Hsing-Chieh , Chiu Weihsueh A , Villenave Remi , Hewitt Philip , Ferguson Stephen S , Rusyn Ivan 
Published: January 2025

Abstract
Intestinal absorption is a key toxicokinetics parameter. Although the colon carcinoma cell line Caco-2 is the most used in vitro model to estimate human drug absorption, models representing other intestinal segments are available. We characterized the morphology, tissue-specific markers, and functionality of 3 human intestinal cell types: Caco-2, primary human enteroid-derived cells from jejunum (J2), and duodenum (D109) when cultured in the OrganoPlate 3-lane 40 microphysiological system (MPS) or static 24-well Transwells. In both conditions, J2 and D109 formed dome-like structures; Caco-2 formed uniform monolayers. In MPS, only Caco-2 formed tubules. Cells grown on Transwells formed a thicker monolayer. All cells and conditions exhibited expression of ZO-1 (tight junctions). Polarization markers Ezrin and Villin were highest in J2 and D109 in MPS, highest expression of Mucin was observed with J2. However, J2 and D109 exhibited poor barrier (70 kDa TRITC-dextran) in MPS, whereas robust barrier was recorded in Transwells. Barrier function and drug transport were evaluated using caffeine, indomethacin, and propranolol. The gel lane in MPS acted as a blockade; only a small fraction crossed, even without cells. The permeability ratios were used to parameterize the probabilistic compartmental absorption model to determine whether in vitro data could reduce uncertainty. The most accurate prediction of the fraction absorbed was achieved with Transwell-derived data from Caco-2, combined with the experimentally derived segment-specific absorption ratios. The impact of this study includes demonstration that enteroid-derived cells cultured in MPS show most physiological morphology, but that studies of drug permeability in this MPS are challenging.
METTL3 promotes renal ischemia‐reperfusion injury by modulating miR‐374b‐5p/SRSF7 axis
Authors: Zhu Kun , Sun Shirui , Li Zepeng , Deng Ge , Guo Yingcong , Zheng Bingxuan , He Qi , Zhao Zhenting , Ding Chenguang 
Published: January 2025

AbstractRenal ischemia–reperfusion injury (IRI) is a prevalent cause of acute kidney injury, however, the regulatory mechanisms of miR‐374b‐5p in renal IRI remain poorly understood. We established hypoxia/reoxidation (H/R)‐induced renal injury models using HK‐2 and TCMK‐1 cells, as well as an ischemia–reperfusion (I/R)‐induced mouse model. Renal tubular epithelial cells (RTECs) viability and apoptosis were assessed using CCK‐8, flow cytometry, and TUNEL assays. The targeting relationship between miR‐374b‐5p and SRSF7 was analyzed using dual luciferase reporter assays. The interaction between METTL3 and miR‐374b‐5p was confirmed through methylated RNA immunoprecipitation (MeRIP) and co‐immunoprecipitation (Co‐IP) assays. We found that miR‐374b‐5p levels were significantly upregulated in H/R‐induced HK‐2 and TCMK‐1 cells. Furthermore, miR‐374b‐5p promoted H/R‐induced RTEC injury by suppressing cell viability and exacerbating apoptosis. SRSF7 was identified as a downstream target of miR‐374b‐5p, inhibition of SRSF7 reversed the inhibitory effects of miR‐374b‐5p inhibitors on RTEC injury. Additionally, METTL3 interacted with the microprocessor protein DGCR8 and modulated the processing of pri‐miR‐374b‐5p in an m6A‐dependent manner. In the renal IRI model, METTL3 and miR‐374b‐5p levels were upregulated, and knockdown of METTL3 inhibited apoptosis in H/R‐induced HK‐2 and TCMK‐1 cells. Conversely, miR‐374b‐5p reversed the protective effects of METTL3 knockdown on renal IRI. Our findings provide novel insights into the role of m6A methylation in the development of renal IRI, demonstrating that METTL3 promotes renal IRI by modulating the miR‐374b‐5p/SRSF7 axis.
Trends and Disparities in Acute Lymphoblastic Leukemia–Related Mortality in the United States from 1999 to 2020
Authors: Ahmed Shahzaib , Ahmad Eeman , Ashraf Hamza , Ashfaq Haider , Akram Umar , Ahmad Shoaib 
Published: January 2025

Objectives:
The incidence of acute lymphoblastic leukemia (ALL) shows a bimodal distribution, with the first peak in children under 10 years old and the second in adults. It is imperative to understand disparities in ALL-related mortality.

Methods:
ALL-related mortality trends in the United States from 1999 to 2020 were studied by extracting age-adjusted mortality rates (AAMRs) from the Centers for Disease Control and Prevention Wide-Ranging Online Data for Epidemiologic Research database. Changes in AAMR were evaluated by calculating annual percentage change (APC) and average APC using Joinpoint regression.

Results:
A total of 35,056 ALL-related deaths were reported. The AAMR declined from 1999 to 2020 (APC: −0.65). Men exhibited a higher AAMR (0.59) than women (0.43). Hispanic or Latinos exhibited the highest AAMR (0.75), followed by non-Hispanic (NH) whites (0.47), NH black or African Americans (0.37), and NH Asian or Pacific Islanders (0.35). Among census regions, the West was observed to have the highest AAMR (0.59), followed by the South (0.49), the Midwest (0.47), and the Northeast (0.45). California had the highest AAMR (0.64), while the District of Columbia had the lowest (0.40). Stratification by urbanization revealed a higher overall AAMR in rural areas (0.52) than in urban areas (0.48). A majority of the deaths occurred in medical facilities (63.52%).

Conclusions:
Even though a decrease was observed in ALL-related mortality in the United States from 1999 to 2020, disparities were identified in trends stratified by sex, race, census regions, and urbanization. It is essential to direct efforts towards high-risk populations to ensure a decrease in ALL-related mortality across the board.
Clinical Evaluation of Nd:YAG Capsulotomy on the Light-Adjustable Intraocular Lens
Authors: Abing Ashley A. , Lee Jimmy K. , Tran Dan B. , Marvasti Amir H. 
Published: January 2025

Purpose:
To investigate the safety and refractive outcomes of neodymium-doped yttrium-aluminum-garnet (Nd:YAG) capsulotomy in pseudophakic eyes with the Light Adjustable Lens (LAL) both before and after completing the light delivery device (LDD) lock-in treatments.

Setting:
Private practice in Orange, California, USA.

Design:
Retrospective, comparative study.

Methods:
Patients were divided into two groups according to the timing of their YAG capsulotomy (Group I underwent YAG capsulotomy before lock-in treatments and Group II underwent YAG capsulotomy upon completion of lock-in treatments). Sphere, cylinder, axis, spherical equivalent, uncorrected visual acuity, and best-corrected visual acuity were compared pre- and postoperatively using two-tailed paired t-tests. Postoperative data were measured one month after Nd:YAG capsulotomy. The alpha level (type I error) was set at 0.05.

Results:
The LAL was successfully implanted in 485 eyes from January 2020 to March 2023. Ten eyes (0.02%) needed YAG capsulotomy prior to the completion of lock-in treatments (Group I) and 28 eyes (5.77%) needed YAG capsulotomy after the lock-in was completed (Group II). Postoperative BCVA significantly improved compared to preoperative values in both groups. Changes in sphere, cylinder, axis, SE, and UCVA pre- and postoperative measurements were not statistically significant (p > 0.05) in both groups.

Conclusions:
YAG capsulotomy can be safe and effective for patients with the LAL who develop PCO during the adjustment period. There were no significant changes in sphere, cylinder, axis, or spherical equivalence after YAG capsulotomy in both groups.
ECPR combined with CRRT successfully rescues a patient who experienced sudden cardiac arrest for 152 minutes: A case report
Authors: Wang Ya-Dong , Lin Jin-Feng , Cao Zhi-Long , Zhang Su-Yan , Han Xu-Dong 
Published: January 2025

Rationale:
Cardiac arrest (CA) is a life-threatening event with a high mortality rate, and neurological injury following cardiopulmonary resuscitation (CPR) is a leading cause of death and disability in survivors. While prolonged CPR is often associated with poor neurological outcomes, there is limited evidence of successful recovery following extended resuscitation efforts. This study aims to highlight the potential for recovery after prolonged CPR by reporting a case of a patient who underwent 152 minutes of CPR, regained consciousness, and made a full recovery. The purpose is to explore whether advanced life-support techniques, such as extracorporeal CPR (ECPR), can improve survival and neurological outcomes even after prolonged CA.

Patient concerns:
A 53-year-old man with no prior health issues experienced sudden CA while exercising and underwent prolonged CPR.

Diagnoses:
Restoration of spontaneous circulation following CA and ventricular fibrillation.

Interventions:
ECPR, target temperature management, continuous renal replacement therapy, and intracranial pressure management.

Outcomes:
Immediate recovery: following the restoration of spontaneous circulation, the patient was immediately transferred to the intensive care unit for further treatment. Despite the prolonged CPR duration, the patient remained hemodynamically stable and was able to tolerate the intensive interventions. Neurological recovery: after 1 week of intensive therapy, the patient regained consciousness. Initially, there was some confusion and disorientation, but he gradually became fully alert, oriented, and communicative. Neurological assessments indicated no significant long-term deficits, and brain imaging showed no signs of irreversible damage. Cardiological and renal recovery: cardiac function was closely monitored, with no evidence of significant ischemic damage to the myocardium. The patient’s renal function improved with continuous renal replacement therapy, and kidney function returned to normal following the discontinuation of dialysis. Discharge: after 2 weeks of treatment in the intensive care unit and a transfer to the cardiology department for rehabilitation, the patient was discharged from the hospital. He had fully recovered both neurologically and physiologically, with no residual deficits.

Lessons:
This case demonstrates that prolonged CPR, when combined with advanced interventions such as ECPR, can result in favorable outcomes, including survival and neurological recovery. The findings suggest that with timely and appropriate treatment, even patients with extended resuscitation efforts may achieve full recovery, thus underscoring the potential of ECPR as a critical life-saving intervention in cases of prolonged CA.
Factors predicting asymptomatic splenic artery aneurysm expansion in patients managed conservatively: A single-center, retrospective, observational study
Authors: Ganbold Khaliun , Jang Youngjin , Mukhtar Yerkyebulan , Ko Gi-Young , Gwon Jun Gyo , Han Youngjin , Cho Yong-Pil 
Published: January 2025

Although splenic artery aneurysms (SAAs) are relatively rare in the general population, they represent the most prevalent type among visceral artery aneurysms. This study aimed to identify predictors of SAA expansion during follow-up and to contribute to a more comprehensive understanding of the natural history of SAAs. This single-center, retrospective, observational study included 137 patients with SAAs who were managed conservatively with computed tomography follow-up for a minimum of 1 year. The primary outcome was sac expansion, defined as any increase in the maximum sac diameter (≥1 mm) observed on follow-up computed tomography. The relationships between clinical variables and SAA expansion were examined. The incidence of SAA expansion was 35.0% (48/137). Eligible patients were categorized into 2 groups: the no-change (n = 89, 65.0%) and expansion (n = 48, 35.0%) groups. The 2 groups did not exhibit significant differences in demographic characteristics or risk factors, except for a higher likelihood of current smoking and portal hypertension in the expansion group. The prevalence of SAA wall calcification was notably higher in the no-change group (41/89 [46.1%] vs 8/48 [16.7%]; P
< .01). Multivariable Cox proportional hazards modeling revealed that SAA expansion was more likely to occur in current smokers (hazard ratio [HR], 4.34 [95% confidence interval [CI], 1.41–13.34]; P = .01) and in those with an initial maximum SAA diameter >14 mm (HR, 3.13 [95% CI, 1.61–6.08]; P < .01), but expansion was less likely to occur in patients with SAA wall calcification (HR, 0.27 [95% CI, 0.12–0.61]; P
< .01). SAA expansion was associated with wall calcification and initial maximum SAA diameters >14 mm. Further larger-scale studies are required to ascertain risk factors for rapid expansion, which could ultimately identify which categories of patients benefit most from early prophylactic intervention.
Dual sensory loss and cognitive impairment: A study in elderly users of gerontological centers in a Galician urban area
Authors: Vázquez-Sánchez Covadonga , Gigirey Prieto Luz M. , del Oro-Sáez Carlos P. 
Published: January 2025

SIGNIFICANCE
Several studies indicate that dual sensory loss (DSL) is related to cognitive impairment. However, there are still no conclusive data on the role of each individual deficit on cognitive performance when both co-occur in the same person.

PURPOSE
This study aimed to analyze the possible association between sensory deficit and cognitive impairment in a sample of older adults and whether this association differs in subjects with DSL compared with those with single or no sensory impairment.

METHODS
Participants of this study were older adult users of gerontological centers in an urban area of Galicia (Spain). All subjects underwent visual and auditory screening tests to detect the presence of DSL. The Spanish version of the Mini-Mental State Examination test was used to identify cognitive impairment. Hearing loss was defined according to Ventry and Weinstein's criteria. Visual impairment was defined as a binocular presenting visual acuity <0.5 (20/40). The presence of DSL was established on the basis of the definitions of the single deficits.

RESULTS
A total of 534 subjects were examined. Older adults with DSL had the worst results in the Mini-Mental test, and those without sensory impairment had the best. The likelihood of cognitive impairment was greater in older adults with DSL than in those without sensory impairment (odds ratio, 6.06 [3.14 to 11.65]). When only visual impairment was present, the likelihood of cognitive impairment was 3.73 times higher (0.95 to 3.09) than that of subjects with no sensory impairment. Hearing loss alone seems not to be significantly associated with a higher risk of cognitive impairment.

CONCLUSIONS
The results of this study highlight the importance of continuing research in DSL for the prevention of cognitive impairment, the design of intervention plans, and the optimization of health resources.
Cholangiocytes’ Primary Cilia Regulate DNA Damage Response and Repair
Authors: Peixoto Estanislao , Pant Kishor , Richard Seth , Abrahante Juan E. , Czaja Wioletta , Gradilone Sergio A. 
Published: January 2025

ABSTRACTPrimary cilia have been considered tumor-suppressing organelles in cholangiocarcinoma (CCA), though the mechanisms behind their protective role are not fully understood. This study investigates how the loss of primary cilia affects DNA damage response (DDR) and DNA repair processes in CCA. Human cholangiocyte cell lines were used to examine the colocalization of DNA repair proteins at the cilia and assess the impact of experimental deciliation on DNA repair pathways. Deciliation was induced using shRNA knockdown or CRISPR knockout of IFT20, IFT88, or KIF3A, followed by exposure to the genotoxic agents cisplatin, methyl methanesulfonate (MMS), or irradiation. Cell survival, cell cycle progression, and apoptosis rates were evaluated, and DNA damage was assessed using comet assays and γH2AX quantification. Anin vivoliver-specificIFT88knockout model was generated using Cre/Lox recombination. Results showed that RAD51 localized at the cilia base, while ATR, PARP1, CHK1 and CHK2 were found within the cilia. Deciliated cells displayed dysregulation in critical DNA repair. These cells also showed reduced survival and increased S-phase arrest after genotoxic challenges as compared to ciliated cells. Enhanced DNA damage was observed via increased γH2AX signals and comet assay results. An increase in γH2AX expression was also observed in ourin vivomodel, indicating elevated DNA damage. Additionally, key DDR proteins, such as ATM, p53, and p21, were downregulated in deciliated cells after irradiation. This study underscores the crucial role of primary cilia in regulating DNA repair and suggests that targeting cilia-related mechanisms could present a novel therapeutic approach for CCA.New and Noteworthy: Our findings reveal a novel connection between primary cilia and DNA repair in cholangiocytes. We showed that DDR and DNA repair proteins localize to cilia, and that deciliation leads to impaired cell survival and S-phase arrest under genotoxic stress. Deciliated cells exhibit heightened DNA damage, evidenced by increased γH2AX signals and comet assay results, a phenotype mirrored inin vivoIFT88 knockout mice. Furthermore, key DDR regulators, including ATM, p53, and p21, are downregulated in deciliated cells following irradiation, highlighting a crucial role for primary cilia in maintaining genome stability.
A numerical solution method for the fractional moment problem within engineering
Authors: Wang Yifan , Tani Ryuichi , Uchida Kenetsu 
Published: January 2025

PurposeIn the field of engineering, the fractional moments of random variables play a crucial role and are widely utilized. They are applied in various areas such as structural reliability assessment and analysis, studying the response characteristics of random vibration systems and optimizing signal processing and control systems. This study focuses on calculating the fractional moments of positive random variables encountered in engineering. This study focuses on calculating the fractional moments of positive random variables encountered in engineering.Design/methodology/approachBy integrating Laplace transforms with fractional derivatives, both analytical and practical numerical solutions are derived. Furthermore, specific practical application methods are provided.FindingsThis approach allows for the stable and highly accurate calculation of fractional moments based on the integer moments of random variables. Data experiments included in this study demonstrate the effectiveness of this method in solving fractional moment calculations in engineering. Compared to traditional methods, the proposed method offers significant advantages in stability and accuracy, which can further advance research in the engineering field that employs fractional moments.Originality/value(1) Accuracy: Although the proposed method does involve some error, its error level is significantly lower than traditional methods, such as the Taylor expansion method. (2) Stability: The computational error of the proposed method is not only minimal but also remains stable within a narrow range as the fractional order varies. (3) Efficiency: Compared to the widely used Taylor expansion method, the proposed method requires only a minimal number of integer-order moments to achieve the desired results. Additionally, it avoids convergence issues during computation, greatly reducing computational resource requirements. (4) Simplicity: The application steps of the proposed method are very straightforward, offering significant advantages in practical applications.
Analysis and manufacturing of truck trailer supports in polyamide PA6
Authors: Amroune Salah , Saib Cherif , Houari Amin , Zergane Said , Mohamad Barhm Abdullah , Madani Kouider , Aichouche Mohamed Lamin , Zidane Madi 
Published: January 2025

PurposeThis study aims to develop high-performance truck trailer supports using polyamide PA6, a thermoplastic material renowned for its mechanical strength, durability and adaptability to extreme conditions. By analysing the material’s properties and exploring manufacturing methods such as injection moulding and 3D printing, the research seeks to optimise the design and production of supports capable of withstanding heavy-duty mechanical stresses. The ultimate goal is to provide the transport industry with reliable, cost-effective and durable solutions that meet safety standards and performance demands, ensuring enhanced operational efficiency and resilience in challenging environments.Design/methodology/approachThe study investigates the design and production of truck trailer supports using polyamide PA6, chosen for its high mechanical strength, thermal stability, and resistance to wear and chemical exposure. The research evaluates manufacturing techniques, including injection moulding and 3D printing, to optimise part performance and durability under heavy-duty mechanical stresses. Emphasis is placed on designing supports capable of enduring harsh environmental conditions while ensuring safety and cost-efficiency. By leveraging the superior properties of PA6, the study aims to develop reliable solutions that meet the transport industry’s stringent performance and economic requirements.FindingsThe study confirms that using PA6 polyamide for truck trailer supports is an innovative and cost-effective solution, offering superior mechanical performance and environmental resistance. A detailed analysis of PA6 properties and manufacturing methods reveals its suitability as a robust and durable alternative for trailer supports. This integration marks a significant advancement in improving trailer efficiency and performance while maintaining quality standards. The findings underscore the importance of leveraging technological advancements to address challenges in innovation and sustainability, supporting the growth of the transport and logistics sector.Originality/valueThis study introduces an innovative approach to designing truck trailer supports by utilising polyamide PA6, highlighting its superior mechanical properties and environmental resilience. By combining material analysis with advanced manufacturing techniques, such as injection moulding and 3D printing, the research provides a novel framework for producing durable, cost-effective components tailored to the transport industry’s demands. The originality lies in the integration of PA6 into heavy-duty applications, showcasing its potential to enhance performance, efficiency and sustainability. This work adds significant value by addressing critical industry challenges and offering practical solutions that align with modern safety and environmental standards.