bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperinsulinemic milieu elicits glomerular podocyte impairment and dysfunction via inducing GSK3beta hyperactivity
Authors: Chen Mengxuan , Ge Yan , Dworkin Lance , Gong Rujun 
Published: December 2023

Background: Epidemiological evidence suggests that hyperinsulinemia or insulin resistance is a significant risk factor for the development of diabetic complications such as DKD. However, whether hyperinsulinemia per se plays a causative role in the development of diabetic kidney injury is unknown and was explored here.
Methods: Pre-diabetic db/db mice were examined for serum insulin levels, urinary albumin to creatinine ratios and renal histology. Conditionally immortalized mouse podocytes were cultured under non-permissive conditions and exposed to high ambient insulin conditions, following GSK3beta silencing, ectopic expression of a constitutively active GSK3beta mutant (S9A), or treatment with a small molecule GSK3beta inhibitor tideglusib. Podocyte injury was assessed and signaling pathways examined.
Results: In pre-diabetic db/db mice, hyperinsulinemia was evident and associated with microalbuminuria and early signs of podocyte impairment, including diminished expression of homeostatic marker proteins like synaptopodin, as compared with db/m littermates. In vitro, prolonged exposure of differentiated podocytes to high ambient insulin induced podocytopathic changes, including cellular hypertrophy, loss of synaptopodin, and disruption of actin cytoskeleton integrity. This was associated with a desensitized insulin signaling and diminished inhibitory phosphorylation of GSK3beta, denoting GSK3beta hyperactivity. In pre-diabetic db/db mice, GSK3beta hyperactivity was confirmed in glomerular podocytes, correlating with the level of hyperinsulinemia or microalbuminuria. In cultured podocytes, ectopic expression of S9A caused podocyte hypertrophy and podocytopathic changes, reminiscent of the harmful effect of the hyperinsulinemic milieu. Conversely, GSK3beta knockdown mitigates podocyte injury elicited by hyperinsulinemic milieu. This protective effect was mimicked by the small molecule inhibitor tideglusib.
Conclusions: GSK3beta hyperactivity is required and sufficient for Hyperinsulinemic milieu-elicited glomerular podocyte impairment and dysfunction.
Unveiling the Silent Constrictor: A Case Report of Takayasu Arteritis Manifesting as a Vascular Enigma
Authors: Pasadyn, BA Vanessa , Davis, MD Samantha , Fatima, MD Rawish , Altorok, MD Nezam 
Published: December 2023

Introduction
Takayasu arteritis (TKA) is a rare large vessel vasculitis, most prevalent in Asia and affecting females ages 10-40 years. Through unclear etiology, it triggers chronic granulomatous inflammation leading to vessel wall thickening, stenosis, and occlusion. Manifestations vary from mild malaise to severe ischemic complications. Diagnosis hinges on clinical criteria, including angiography, and exclusion of mimicking conditions like giant cell arteritis, fibromuscular dysplasia, or atherosclerosis. Treatment necessitates immunosuppression and anti-inflammatories to curb disease progression and limit complications.
Case Presentation
A 61-year-old Caucasian female presented with claudication of the upper extremities upon using the shower. She reported episodes of headache, myalgia, fever and chills of several months. She reported fatigue with overhead reaching and difficulty finding her pulse on her upper extremities. Labs were notable for elevated ESR and CRP. MRA chest showed smoky thickened appearance of the descending aorta wall, some missing wall thickening in origins of the left common carotid and left subclavian arteries. PET scan revealed diffuse hypermetabolic activity involving the bilateral common carotid arteries, peripheral aortic arch and descending thoracic aorta (Figure 1). With these ongoing symptoms and characteristic features on imaging, we established the diagnosis of TKA. She was started on 5mg oral prednisone daily and methotrexate 10mg weekly with daily folic acid.
Conclusion
This case highlights the diagnosis and treatment of a rare condition, Takayasu arteritis, the pulseless disease. TKA is a challenging condition to study and understand fully, but advancements in medical knowledge, imaging, and management are gradually improving our understanding and ability to diagnose and treat this condition. With increased awareness of this condition, earlier detection and monitoring will serve to benefit future patient populations.
Protocol Development for Yielding High Quality DNA and RNA from Archived Formalin-Fixed Paraffin Embedded Tissues
Authors: Kazmi Sara , Khatib-Shahidi Bella , Najjar John , Sharma Anish , Murphy Caitlyn , Bashir Humza , French Benjamin W. , Lad Apurva , Kennedy David , Haller Steven 
Published: December 2023

Introduction: While genetic analysis of archived formalin-fixed paraffin embedded (FFPE) tissue specimens would be a significant research resource, extraction of high-quality DNA and RNA from these specimens is a significant challenge. Storage duration, tissue handling and tissue preservation processes as well as their interactions with the nucleic acids could influence the quality and integrity of the DNA or RNA required for genetic analysis.
Objectives: The goal of this study was to establish a protocol to extract high quality DNA and RNA from the FFPE tissues for further use in quantitative PCR analysis.
Methods: For the purposes of this study, human FFPE biopsy tissues from lung, liver, colon and kidney were obtained from a single center biorepository. GeneJET Genomic DNA Purification Kit (Catalog # K0722) obtained from Thermo Fisher Scientific and RecoverAll Total Nucleic Acid Isolation Kit obtained from Life Technologies were modified and used for extraction of DNA and RNA, respectively. Briefly, modifications involved extending reagent incubation times, increasing sample volumes and wash steps, and increased final nucleic acids recovery and concentration steps. Eight sections around 8-10 ?m thick were microtomed for each tissue sample and used for extraction. The purity of the nucleic acids obtained was verified using Nanodrop Spectrophotometer.
Results: The average DNA yield from eight sections for each of the tissues was 270±184 ng/?l and for RNA was 296±188 ng/?l. Nucleic acid quality was assessed by measuring the 260nm/280nm absorbance ratio for protein contamination as well as the 260nm/230nm absorbance ratio for salt contamination. Both were found to be within acceptable ranges. RNA was reverse transcribed to cDNA and qPCR was successfully performed on both DNA and cDNA samples.
Conclusions: These results indicate that protocols using the silica-based membrane technology can yield high quality DNA and RNA that can be successfully used for downstream genetic analysis.
ŞİRKETLER HUKUKUNDA TAHKİM
Authors: ŞAHİN ALTINTAŞ Mehtap 
Published: December 2023

Devlet yargısına alternatif uyuşmazlık çözüm yöntemlerinden biri olan ve hukuk sistemimizde 160 yıllık geçmişi bulunan tahkim, değişen ve gelişen ihtiyaçlar neticesinde günümüzde daha fazla önem kazanmış ve çeşitli alanlarda uygulanmaya başlanmıştır. Bu alanların başında, iş dünyasının hızlı ve uzmanlık gerektiren çözümlere ihtiyaç duyduğu ve dolayısıyla özel bir hukuk dalı olarak değerlendirilen şirketler hukuku gelmektedir. Bu çalışma, şirketler hukuku uyuşmazlıklarının tahkime elverişliliğini değerlendirmeyi amaçlamaktadır. Bu bağlamda doktrindeki farklı görüşler ve yargı kararları çerçevesinde şirketler hukukundan doğan uyuşmazlıkların tahkime elverişlilik analizi yapılmıştır.
Amaç: Çalışmada alternatif uyuşmazlık yöntemlerinden biri olan tahkim yönteminin Türk hukukunda düzenleniş biçiminden yola çıkılarak, 6102 sayılı Türk Ticaret Kanunu’nun emredici hükümleri doğrultusunda şirketler hukukunda ortaya çıkan uyuşmazlıkların tahkime elverişli olup olmadığı hususunun doktrin görüşleri ve yargı kararları ışığında detaylıca incelenmesi amaçlanmıştır.
Yöntem: Çalışmada tahkime ilişkin genel hususlar içeren 4686 sayılı Milletlerarası Tahkim Kanunu ile 6100 sayılı Hukuk Muhakemeleri Kanunu’nun tahkime elverişlilik hususunda ortaya koyduğu ölçüler belirlenerek, şirketler hukuku pratiklerinde ortaya çıkan uyuşmazlıkların bu ölçüler uyarında tahkime elverişli olup olmadığı hususu, farklı yaklaşımlar üzerinden, mukayeseli hukuktaki gelişmelere de atıf yapılarak ele alınmıştır.
Bulgular: Taraf iradelerinin büyük ölçüde belirleyici olduğu ticaret hukuku uyuşmazlıklarında tahkime duyulan ihtiyaç ortada olmakla birlikte; şirketler hukuku uyuşmazlıklarının üçüncü kişiler üzerindeki etkilerinin iradîlik unsuru bakımından ortaya çıkardığı tereddütlerin yanı sıra, bu alanda mevzuatta açık düzenlemelerin bulunmaması, tahkime temkinli yaklaşılmasına sebebiyet vermiştir. Bununla birlikte doktrinde ortaya atılan çözümlerle iradîlik unsurunun sağlanması mümkün olurken, mukayeseli hukukta olduğu gibi Türk hukukunda da bu alanda açık düzenlemelerin yapılacağı düşünülmektedir. Böylelikle tahkimin hızlı ve uzmanlık gerektiren bu uyuşmazlıklara dair çözümleri ile şirketler hukukunda tahkimin yaygınlaşmaya başladığı söylenebilmektedir.
Özgünlük: Hukukçular tarafından şirketler hukukunda tahkime ilişkin oldukça zengin bir mecrada birçok akademik çalışma bulunmasına rağmen, en sık yaşanan uyuşmazlıklara doktrinde üretilen çözümlerin sentezlendiği bu çalışmada konunun farklı bir perspektifle ele alınması çalışmaya özgünlük kazandırmış olup, bu yönüyle literatüre katkı sağlanması amaçlanmıştır.
Anahtar Kelimeler: Tahkim, Tahkime Elverişlilik, Şirketler Hukuku, Emredicilik, Tek Borç İlkesi.
Jel Sınıflandırması: K12
PENGARUH GAME ONLINE TERHADAP HASIL BELAJAR PESERTA DIDIK DI SMP NEGERI 4 BANJARBARU
Authors: Rahmawati Siti , Filardi Filardi 
Published: December 2023

AbstractThis study discusses "The Effect of Online Games on Student Learning Outcomes at SMP Negeri 4 Banjarbaru". The purpose of this study was to find out how high online game addiction is, how high the learning outcomes of participants are, whether there is an effect of online games on students and to find out how much influence online games have on learning outcomes at SMP Negeri 4 Banjarbaru, South Kalimantan Province. The subjects in this study were students at SMP Negeri 4 Banjarbaru which was more specialized, namely class VII, while the object in this study was the influence of online games on student learning outcomes at SMP Negeri 4 Banjarbaru. In extracting data the author uses a questionnaire, observation, and interviews. While the data analysis technique uses a quantitative approach using product moment analysis techniques. Based on the results of this study, it was concluded that online gaming activities at SMP Negeri 4 Banjarbaru were in the high category, the learning outcomes of students at SMP Negeri 4 Banjarbaru were in the high category, thus there was an influence between online games and student learning outcomes.Keywords: Influence, Learning Outcomes, Online Games.AbstrakPenelitian ini membahas tentang “ Pengaruh Game Online Terhadap Hasil Belajar Peserta Didik Di SMP Negeri 4 Banjarbaru”. Tujuan penelitian ini ialah untuk mengetahui seberapa tinggi kecanduan game online, seberapa tinggi hasil belajar peserta, apakah ada pengaruh game online terhadap peserta didik dan Untuk mengetahui seberapa besar pengaruh game online terhadap hasil belajar di SMP Negeri 4 Banjarbaru Provinsi Kalimantan Selatan. Subjek dalam penelitian ini adalah peserta didik di SMP Negeri 4 Banjarbaru yang lebih dikhususkan yaitu kelas VII, sedangkan objek dalam penelitian ini adalah Pengaruh Game Online Terhadap hasil belajar Peserta didik di SMP Negeri 4 Banjarbaru. Dalam penggalian data penulis menggunakan angket, observasi, dan wawancara. Sedangkan teknik analisis data menggunakan pendekatan kuantitatif dengan menggunakan teknik analisis product moment. Berdasarkan hasil penelitian ini memperoleh kesimpulan bahwa Aktivitas game online di SMP Negeri 4 Banjarbaru dalam kategori tinggi, Hasil belajar peserta didik di SMP Negeri 4 Banjarbaru dalam kategori tinggi, dengan demikian terdapat pengaruh antara game online dengan hasil belajar peserta didik.Kata Kunci: Hasil Belajar, Game Online, Pengaruh.
BANDA ORTODÔNTICA COMO POSSIBILIDADE RESTAURADORA PARA GRANDES DESTRUIÇÕES CORONÁRIAS EM ODONTOPEDIATRIA: RELATO DE CASO
Authors: Alves Gomes Kleber , Antunes Custódio Beatriz , Paula Novato Campos Brennda , Melo Santos Rafaela , Lima Rodrigues Laila , Teixeira Oliveira Bianca , Alana Castro Brasil Flávia , Caroline Alves Duarte Ana 
Published: December 2023

O objetivo deste trabalho é relatar um caso clínico de cimentação de banda ortodôntica como opção restauradora para grandes destruições coronárias em molares decíduos. Os dados foram obtidos por meio do prontuário da paciente e dos registros fotográficos realizados durante as três sessões, incluindo: consulta, tratamento e acompanhamento. A paciente do sexo feminino de 04 anos, apresentava extensa destruição coronária causada por lesão cariosa profunda. Foi ponderado que a melhor opção para o caso seria a cimentação da banda ortodôntica de modo a preservar o remanescente dentário. Dessa forma, pôde ser confirmado na descrição desse estudo que a cimentação da banda ortodôntica em coroas de dentes decíduos bastante desmineralizadas é uma alternativa de tratamento reabilitador na primeira infância.
The hyperbolic sets with the fitting shadowing property
Authors: AL-Ftlawy Dunia M. K. AL-Ftlawy , AL-Shara’a Iftichar M. T. 
Published: December 2023

We explain around C1-stable fitting shadowing property of a diffeomorphism f of C∞ (closed manifold M) denote by SFSP of invariant closed set B in M; we want to prove the main Theorem that f_(|B) satisfies a C^1- stable fitting shadowing property if and only if B is a hyperbolic set with the same index; if partially hyperbolic diffeomorphisms map contains q, r are types of saddle points with different indices, then f does not investigate the fitting shadowing property.
A fractional models of some partial differential equations and analytical approximate solution arising in mathematical sciences
Authors: Hussein Qays Jasem , Altaie Huda Omran 
Published: December 2023

The present paper, a combination of Sumudu Adomian Decomposition approach (STADM)is apply to investigate the analytical solutions for some Fractional Differential Equations(FDEs). The STADM is a new technique using the starting causes. To evaluate the effectiveness and powerful of the suggested technique, several applications for examples are provided to get analytical or numerical results of Gas Dynamics equation, Fornberg - Whitham equation and Klein- Gordon equation. The Caputo sense definition applies to fractional derivatives. In addition, the approaches that were proposed gave convergence series solutions that had components that were simple to determine, and they did so without making use of perturbation or linearization.
A INVISIBILIDADE PERIFÉRICA E O PLURALISMO JURÍDICO
Authors: Santos Genilda José dos , Lima Alba Cristina Teixeira , Oliveira Claudilene Jordão da Costa , Alencar Maria de Fátima Ramos Torres 
Published: December 2023

O objetivo do presente artigo é analisar o pluralismo jurídico como alternativa ao direito positivado, como um contraponto à hegemonia jurídica da classe economicamente dominante e detentora do conhecimento científico, jurídico sufocando as alternativas, os saberes dos povos originais, das favelas, dos expropriados da terra, dos não representados pela sociedade. A ideia da sociedade como vontade política de muitos, não chega a ser efetivamente uma sociedade para todos, é seletiva e elitista, quem decide os rumos que o país e as normas que devemos seguir é um pequeno grupo bancado pelos detentores do capital, banqueiros, empresários, grandes corporações até mesmo internacionais que financiam a classe política desde que defendam seus interesses econômicos e ideológicos de perpetuar a desigualdade social, a segregação entre ricos e pobres, brancos e negros, heterossexuais e homossexuais, evangélicos e católicos e outras religiões. Quando a Constituição Federal de 1988 diz todos são iguais perante a lei, se fôssemos esmiuçar essa igualdade desde o berço, veríamos uma desigualdade abissal. Há grupos invisíveis na nossa sociedade, grupos sem voz, sem representatividade, sem direitos, são tratados como não cidadãos, ou cidadãos de última categoria, como pudéssemos classificar um ser humano em categorias, isto de per si já é ultrajante, normalizar e normatizar a exclusão social é um atentado a toda humanidade.
In situ measurements of perturbations to stratospheric aerosol and modeled ozone and radiative impacts following the 2021 La Soufrière eruption
Authors: Li Yaowei , Pedersen Corey , Dykema John , Vernier Jean-Paul , Vattioni Sandro , Pandit Amit Kumar , Stenke Andrea , Asher Elizabeth , Thornberry Troy , Todt Michael A. , Bui Thao Paul , Dean-Day Jonathan , Keutsch Frank N. 
Published: December 2023

Abstract. Stratospheric aerosols play important roles in Earth's radiative budget and in heterogeneous chemistry. Volcanic eruptions modulate the stratospheric aerosol layer by injecting particles and particle precursors like sulfur dioxide (SO2) into the stratosphere. Beginning on 9 April 2021, La Soufrière erupted, injecting SO2 into the tropical upper troposphere and lower stratosphere, yielding a peak SO2 loading of 0.3–0.4 Tg. The resulting volcanic aerosol plumes dispersed predominately over the Northern Hemisphere (NH), as indicated by the CALIOP/CALIPSO satellite observations and model simulations. From June to August 2021 and May to July 2022, the NASA ER-2 high-altitude aircraft extensively sampled the stratospheric aerosol layer over the continental United States during the Dynamics and Chemistry of the Summer Stratosphere (DCOTSS) mission. These in situ aerosol measurements provide detailed insights into the number concentration, size distribution, and spatiotemporal variations of particles within volcanic plumes. Notably, aerosol surface area density and number density in 2021 were enhanced by a factor of 2–4 between 380–500 K potential temperature compared to the 2022 DCOTSS observations, which were minimally influenced by volcanic activity. Within the volcanic plume, the observed aerosol number density exhibited significant meridional and zonal variations, while the mode and shape of aerosol size distributions did not vary. The La Soufrière eruption led to an increase in the number concentration of small particles (<400 nm), resulting in a smaller aerosol effective diameter during the summer of 2021 compared to the baseline conditions in the summer of 2022, as observed in regular ER-2 profiles over Salina, Kansas. A similar reduction in aerosol effective diameter was not observed in ER-2 profiles over Palmdale, California, possibly due to the values that were already smaller in that region during the limited sampling period in 2022. Additionally, we modeled the eruption with the SOCOL-AERv2 aerosol–chemistry–climate model. The modeled aerosol enhancement aligned well with DCOTSS observations, although the direct comparison was complicated by issues related to the model's background aerosol burden. This study indicates that the La Soufrière eruption contributed approximately 0.6 % to Arctic and Antarctic ozone column depletion in both 2021 and 2022, which is well within the range of natural variability. The modeled top-of-atmosphere 1-year global average radiative forcing was −0.08 W m−2 clear-sky and −0.04 W m−2 all-sky. The radiative effects were concentrated in the tropics and NH midlatitudes and diminished to near-baseline levels after 1 year.
3D-printed Ag–AgCl electrodes for laboratory measurements of self-potential
Authors: Rowan Thomas S. L. , Karantoni Vilelmini A. , Butler Adrian P. , Jackson Matthew D. 
Published: December 2023

Abstract. This paper details the design, development, and evaluation of a 3D-printed rechargeable Ag–AgCl electrode to measure self-potential (SP) in laboratory experiments. The challenge was to make a small, cheap, robust, and stable electrode that could be used in a wide range of applications. The new electrodes are shown to offer comparable performance to custom-machined laboratory standards, and the inclusion of 3D printing (fused filament fabrication or FFF and stereolithography or SLA) makes them more versatile and significantly less expensive – of the order of ×40 to ×75 cost reduction – to construct than laboratory standards. The devices are demonstrated in both low-pressure experiments using bead packs and high-pressure experiments using natural rock samples. Designs are included for both male and female connections to laboratory equipment. We report design drawings, practical advice for electrode printing and assembly, and printable 3D design files to facilitate wide uptake.
Đặc điểm hóa sinh của bệnh nhân thiếu beta-ketothiolase
Authors: Quỳnh Anh Mai , Mai Trần Thị Chi , Thúy Nguyễn Thị Phương , Đồng Nguyễn Kim 
Published: December 2023

Bệnh thiếu hụt enzym beta-ketothiolase là một bệnh rối loạn chuyển hóa do đột biến gen ACAT1 (gen T2) nằm trên nhiễm sắc thể số 11 (11q22.3-q23). Bệnh được đặc trưng bởi những đợt nhiễm toan ceton cấp và không có triệu chứng lâm sàng giữa các đợt. Cơ chế gây bệnh thiếu enzym beta-ketothiolase là do đột biến gen T2, dẫn tới gián đoạn quá trình giáng hóa isoleucin và thể ceton trong cơ thể. Các xét nghiệm hóa sinh đóng vai trò quan trọng trong việc sàng lọc và chẩn đoán bệnh thiếu hụt enzym beta-ketothiolase. Nghiên cứu hồi cứu, mô tả một loạt ca bệnh được thực hiện nhằm mô tả các đặc điểm sàng lọc và phân tích acid hữu cơ niệu của bệnh nhân thiếu beta-ketothiolase. Định lượng các acylcarnitin trong mẫu máu thấm khô bằng kỹ thuật khối phổ đôi (MS/MS) và phân tích acid hữu cơ niệu bằng kỹ thuật sắc ký khí ghép nối khối phổ (GC/MS) được áp dụng để chẩn đoán 26 bệnh nhân tại Bệnh viện Nhi Trung ương từ tháng 04/2017 đến tháng 01/2023. Trong đó, có 23 bệnh nhân được sàng lọc bằng nồng độ acylcarnitin máu có 19/23 tăng nồng độ của acylcarnitin C5:1 và 20/23 tăng C5:OH, 18/23 bệnh nhân tăng cả 2 chỉ số C5:1 và C5:OH; 1 bệnh nhân chỉ tăng C5:1; 2 bệnh nhân chỉ tăng C5:OH; 2 bệnh nhân không tăng cả 2 chỉ số. Kết quả phân tích acid hữu cơ niệu của 26 bệnh nhân cho thấy sự tăng nồng độ của acid niệu 2MAA (6/26), 2M3HB (23/26), TIG (13/26) ở các bệnh nhân thiếu beta-ketothiolase. Xét nghiệm sàng lọc bằng MS/MS không đảm bảo phát hiện 100% các trưởng hợp bệnh ngay cả khi có triệu chứng lâm sàng. Không phải tất cả các bệnh nhân đều có mẫu hình đặc trưng khi phân tích acid hữu cơ niệu của bệnh thiếu beta-ketothiolase là tăng đào thải cả 3 chất 2MAA, 2M3HB và TIG trong nước tiểu.