bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a Decellularized Multicomponent Extracellular Matrix Interpenetrating Network Scaffold by Gelatin Microporous Hydrogel 3D Cell Culture System
Authors: Shi Junli , Yao Hang , Wang Bowen , Yang Jian , Liu Dianwei , Shang Xianfeng , Chong Hui , Fei Wenyong , Wang Dong‐An 
Published: December 2023

AbstractInterface tissue repair requires the construction of biomaterials with integrated structures of multiple protein types. Hydrogels that modulate internal porous structures provide a 3D microenvironment for encapsulated cells, making them promise for interface tissue repair. Currently, reduction of intrinsic immunogenicity and increase of bioactive extracellular matrix (ECM) secretion are issues to be considered in these materials. In this study, gelatin methacrylate (GelMA) hydrogel is used to encapsulate chondrocytes and construct a phase transition 3D cell culture system (PTCC) by utilizing the thermosensitivity of gelatin microspheres to create micropores within the hydrogel. The types of bioactive extracellular matrix protein formation by chondrocytes encapsulated in hydrogels are investigated in vitro. After 28 days of culture, GelMA PTCC forms an extracellular matrix predominantly composed of collagen type II, collagen type I, and fibronectin. After decellularization, the protein types and mechanical properties are well preserved, fabricating a decellularized tissue‐engineered extracellular matrix and GelMA hydrogel interpenetrating network hydrogel (dECM‐GelMA IPN) consisting of GelMA hydrogel as the first‐level network and the ECM secreted by chondrocytes as the second‐level network. This material has the potential to mediate the repair and regeneration of tendon–bone interface tissues with multiple protein types.
Identification of ATAD3A as a key regulator in non‐small cell lung cancer by promoting STAT3‐induced cell proliferation and tumor angiogenesis
Authors: Jiang Tao , Li Ning , Xu Hao , Sun Li , Zhang Ying , Luo Qian , Yang Li 
Published: December 2023

AbstractMalignant proliferation and abundant angiogenesis are major causes of lung adenocarcinoma (LUAD) with high morbidity and mortality. Therefore, the exploration of the key regulatory mechanisms of malignant proliferation and angiogenesis in LUAD provides an opportunity for the development of targeted precision therapy. In this study, we found that the high expression of ATPase family AAA domain‐containing protein 3A (ATAD3A) in LUAD was positively associated with the poor survival of patients, while its high expression was positively associated with the angiogenesis of LUAD. Further knockdown of ATAD3A in LUAD significantly inhibited cell proliferation and suppressed expression of vascular endothelial growth factor A, FGF‐2, ANG‐1, and TGF‐β. The opposite effect was observed with ATAD3A overexpression. Furthermore, ATAD3A knockdown significantly inhibited tumor growth and angiogenesis in an in vivo subcutaneous xenograft tumor model. Mechanistic studies suggest that ATAD3A may promote signal transducer and activator of transcription 3 activation, a key signal regulating lung cancer cell proliferation and transcriptional secretion of proangiogenic factors. Therefore, targeted inhibition of ATAD3A may be an effective strategy for LUAD therapy, and ATAD3A may be a potential biomarker for predicting malignant progression.
Surface Modification of Lignite with Alkyl and Mixed Alkyl‐Aryl Films Generated from an Aryl Diazonium Salt and Alkyl Halides: Experimental Results and Theoretical Analyses
Authors: Hasani Msc. Gentiana , Berisha Avni , Hetemi. Dardan , Decorse Philippe , Pinson Jean , Podvorica Fetah I. 
Published: December 2023

AbstractIn search of new possible uses of cheap lignite from the Kosova Bassin, the surface of lignite powders is modified with alkyl or mixed alkyl‐aryl layers. Modification is performed in aqueous acid solution containing an aryl diazonium salt and an alkyl halide compound in millimolar concentration, in the presence of potassium iodide as a reducing agent at equimolar concentration. Attachment of alkyl films substituted with carboxylic groups and aryl films with nitro or bis‐trifluoromethyl groups is characterized by IRATR and XPS spectroscopy. The formation of a stable interface during the grafting reactions of alkyl and aryl moieties with lignite surface has been confirmed by theoretical calculations. Aryl diazonium salts once chemically or spontaneously reduced are a source of aryl radicals, able to attach chemically to the material surface or to react with alkyl halides by abstracting the halogen atom. If the aryl diazonium salts are unable to graft to the coal surface due to steric hindrance, they can, nevertheless, abstract an iodine or bromine atom to generate alkyl radicals that react with the material surface.
Pain management considerations in patients living with both pain syndromes and cardiovascular diseases and disorders
Authors: Leppien Emily E. , Pauling Erin E. , Smith Eric , Wisniewski Brady , Carpenter Abigayle , Schwier Nicholas C. 
Published: December 2023

AbstractConcomitant pain syndromes and cardiovascular disease (CVD) and disorders are associated with significant morbidity, impaired quality of life, and neuropsychiatric disorders. There is an interplay between the mechanisms of pathophysiology of both CVD and pain syndromes. Patients with CVD (and/or disorders) as well as pain syndromes have an increased propensity for drug–drug/disease interactions. Therefore, an understanding of how to use pharmacotherapy to treat pain syndromes, in the context of patients who have diagnoses of CVD and/or disorders, is paramount to patients' success in achieving adequate pain control and appropriately managing CVD and/or disorders, all while decreasing the risk of adverse events (AEs) both from pharmacotherapy to treat pain and CVD (and/or disorders). Based on the appraisal of literature and authors' clinical expertise, it was determined that gabapentinoids, opioids, skeletal muscle relaxants, tricyclic antidepressants, clonidine, serotonin norepinephrine‐reuptake inhibitors, dronabinol, carbamazepine, second‐generation antipsychotics, non‐steroidal anti‐inflammatory drugs, aspirin, corticosteroids, and topical anesthetics have the most evidence for use in patients with CVD and/or disorders. However, the literature surrounding the use of pharmacotherapy for pain management is limited to retrospective studies and there is a lack of well‐designed, prospective, randomized trials; this also includes head‐to‐head comparator studies. Unlike many CVD‐related pharmacotherapy studies, data studying pain management in patients with CVD lacks standardized outcomes that are consistent among the pool of data. Overall, the decision to prescribe specific pain management therapies in patients with CVD and/or disorders should include assessment of pain severity, type of pain, drug–drug/disease interactions, adjuvant therapies required, and the risk or presence of AEs.
Evaluation of the safety and tolerability of intravenous undiluted levetiracetam at a pediatric institution
Authors: Price Lily , Garrity Lisa , Stiehl Sarah 
Published: December 2023

AbstractStudy ObjectiveRecent studies suggest rapid administration of high‐dose, undiluted levetiracetam is safe in adults; however, no information exists in pediatric patients. The purpose of this study was to evaluate the safety and tolerability of undiluted levetiracetam at a pediatric institution.DesignRetrospective, single‐center, cohort study.SettingPediatric Academic Medical Center.PatientsAll patients who received high‐dose >60 mg/kg (‒10%) up to 4500 mg undiluted or diluted intravenous levetiracetam were included.InterventionRapid intravenous administration of undiluted versus diluted levetiracetam.Measurements and Main ResultsA total of 776 levetiracetam doses were included, 358 doses administered and 418 doses wasted. The doses administered (61 undiluted and 297 diluted) accounted for a total of 252 patients (39 received undiluted, and 213 received diluted levetiracetam) (median [minimum–maximum range] age, 2 years [1 day to 32.7 years]; mean (standard deviation [SD]) weight, 20.1 kg [22.1 kg]). The incidence of hemodynamic disturbances and infusion‐related reactions was not statistically significant between undiluted (24.6%) and diluted (26.3%) groups (p = 0.87). The median (interquartile range [IQR]) time difference between first‐line antiseizure medication and levetiracetam administration in patients with status epilepticus was 18 min (10.5–30.5) in the undiluted group versus 36.5 min (21.8–67.3) in the diluted group (p < 0.01). Additionally, there was a significant amount of drug waste from dispensed but not administered doses of the diluted bag compared to undiluted vials (57.6% diluted vs. 18.7% undiluted, p < 0.001).ConclusionUndiluted levetiracetam was not associated with an increased incidence of adverse effects compared to diluted levetiracetam in high‐doses, up to 4500 mg given over 5 min in pediatric patients.
A decade of change: The evolution of pharmacy services at U.S. cystic fibrosis centers
Authors: Grube Paige M. , Autry Elizabeth B. , Kormelink Lauren N. , Young David C. , Zobell Jeffery T. , Bhakta Zubin N. , Schadler Aric D. , Kuhn Robert J. 
Published: December 2023

AbstractIntroductionPeople with cystic fibrosis (pwCF) require a multidisciplinary care team due to disease complexity. The Cystic Fibrosis Foundation (CFF) notes that pharmacists are recommended, while other organizations consider pharmacists required. In 2016, the CFF initiated a grant program for CFF‐accredited care centers and affiliate programs (CFF‐ACCAP) to implement outpatient pharmacy services. The primary objective of this study was to compare surveys regarding pharmacy involvement in CFF‐ACCAP pre‐ and post‐grant implementation.MethodsThis was an IRB‐approved, survey‐based study. The surveys were distributed via the CF pharmacist‐pharmacy technician and center director e‐mail exchanges.ResultsThere are currently 244 CFF‐ACCAP and 158 pharmacists. Forty‐two pharmacists completed the 2013 survey and 77 completed the 2023 survey. Practice site shifted from primarily the inpatient (58.5%) to outpatient settings (67.5%; p < .001). Most positions were created in the past 7 years (81%) with 50% currently or previously funded by the CFF grant program. CFF center director response decreased from 2013 to 2023 (106 vs. 48) but centers with a dedicated CF pharmacist increased from 2013 to 2023 (66%–86%; p = .014). In the 2023 survey, we received responses from 17 pharmacy technicians, who were newly included. Most of these technicians (64%) reported working in outpatient clinics.ConclusionsSince 2013, pharmacy presence has grown at CFF‐ACCAP, partly due to the CFF grant program. Despite pharmacists not being required members of the multidisciplinary care team, their presence is notable in 65% of CFF‐ACCAP centers, where they contribute significantly to improving the care provided for pwCF.
Study of extractables and leachables of non‐polyvinyl chloride soft infusion bags from different batches by analytical assessment threshold screening
Authors: Lu Zheng , Sun Xuechun , Debraha Augustine Atta , Du Zhenxia 
Published: December 2023

AbstractThis study utilized ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry and gas chromatography–mass spectrometry techniques to analyze multiple batches of non‐polyvinyl chloride (PVC) soft bags exposed to three extractants. Based on an established library of relative response factors for more than 30 different polymer additives, analytical evaluation thresholds for large volume injectables were determined, and 30 extractables were screened and (semi‐)quantitatively analyzed. To identify variations in extractables between batches, multivariate statistical methods such as principal component analysis, heat map analysis, and hierarchical cluster analysis were employed. Additionally, a comprehensive risk assessment of extractables and a biosafety assessment of leachables were conducted. The results of the study revealed that the concentrations of two extractables exceeded the established safety threshold. Moreover, one of these extractables was deemed to pose a mutagenic risk based on the consistent findings of two (Q)SAR software tools. Notably, certain extractables exhibited discrepancies in their content between batches, with particular emphasis on 1,3‐bis(3,5‐di‐tert‐butyl‐4‐hydroxybenzyl)‐1,3,5‐triazinane‐2,4,6‐trione. This discrepancy led to inconsistencies in risk levels across batches. However, the levels of leachables detected in the non‐PVC soft bags were found to be within the safety threshold, and cytotoxicity assessments consistently yielded negative results for leachables. Consequently, this study provides valuable data for conducting batch stability investigations concerning extractables in large volume parenteral drug delivery containers and other forms of pharmaceutical packaging.
Bacterial biostimulants for climate smart agriculture practices: Mode of action, effect on plant growth and roadmap for commercial products
Authors: Singh Ravinder , Kaur Sehijpreet , Bhullar Sukhveer S. , Singh Hardeep , Sharma Lakesh K. 
Published: December 2023

AbstractAmidst the global food shortage and the global climate change challenge, there is an urgent need to double food production by 2050. However, the modern crop production methods, including the use of fertilizers and pesticides, have adverse environmental consequences, exacerbating the climate crisis. To address this challenge, a transition to sustainable agriculture is imperative that can harmonize the issue. Biostimulants offer an eco‐friendly solution, especially bacterial biostimulants centred on plant growth‐promoting rhizobacteria (PGPRs). These biostimulants hold the promise of offering environmentally sustainable solutions to enhance crop productivity. The adoption of PGPR‐based biostimulants in agriculture has gained significant momentum in agricultural research. PGPRs enhance plant growth through multifaceted mechanisms. This review delves into the various modes of action employed by PGPRs to improve plant growth, including their impact on nutrient availability (such as nitrogen fixation and mineral solubilization) and stress mitigation. In addition, the practical implication of PGPR strains in field research has been discussed extensively. Besides, the review outlines the roadmap for commercializing PGPR‐based biostimulants and discusses the associated challenges and limitations. A balanced perspective on the practical implementation of PGPRs in modern agriculture is presented. Exploration of future strategies and directions rounds out the review, emphasizing the necessity of a comprehensive approach to address research gaps and unlock the full potential of PGPR‐based biostimulants for sustainable agriculture. In conclusion, this review underscores the applicability of PGPR‐based biostimulants as an innovative solution to address the current food crisis in the context of climate change.
Development of New 5‐(Oxazol‐2‐yl)Pyrimidine Derivatives with Promising Anticancer Activities
Authors: Shyam Kumar Gajula , Ramesh Gaddam , Sridhar Gattu , Kiran Gangarapu , Menga Prasanna Sheela , Kalyani Sambaru , Rameshwar Nimma , Shireesha Boyapati , Chandrasekhar Vasam , Satyanarayana Mavurapu 
Published: December 2023

AbstractA novel series of oxazolyl‐pyrimidine derivatives (11 a–j) have been synthesized and structures were confirmed by spectral data. The synthesized compounds were assessed for cytotoxic profile towards human cervix cancer (SiHa), lung cancer (A549), breast cancer (MCF‐7), and colon cancer (Colo‐205) cell lines by employing MTT assay and using etoposide as the positive control. The heteroaryl compounds having the terminal pyridyl (11 a) and thiazolyl (11 b) groups showed superior cytotoxic potency against cervix and lung cancer cell lines and varying methoxy substituted derivatives 11 c–11 e have excellent to good cytotoxic properties. Compound 11 a and 11 b have IC50 values in the range of 0.01±0.0034 to 0.17±0.059 μM and 0.10±0.038 to 0.66±0.077 μM respectively in the tested cell lines. The selected compounds 11 a and 11 b were studied by molecular docking to assess the binding interactions, binding energies, and possible mechanisms. The in‐silico ADME properties were calculated and all the compounds have the desired properties of drug‐like molecules. The toxicity results indicate that all the compounds are free from toxicity except 11 j, which has high tumorigenic properties. The optimized structure, energies of frontier molecular orbitals, molecular electrostatic potential, and NLO parameters for compound 11 a were determined using DFT/B3LYP level with a 6‐311++G(d,p) basis set.
Nanotherapeutic Heterogeneity: Sources, Effects, and Solutions
Authors: Morla‐Folch Judit , Ranzenigo Anna , Fayad Zahi Adel , Teunissen Abraham Jozef Petrus 
Published: December 2023

AbstractNanomaterials have revolutionized medicine by enabling control over drugs’ pharmacokinetics, biodistribution, and biocompatibility. However, most nanotherapeutic batches are highly heterogeneous, meaning they comprise nanoparticles that vary in size, shape, charge, composition, and ligand functionalization. Similarly, individual nanotherapeutics often have heterogeneously distributed components, ligands, and charges. This review discusses nanotherapeutic heterogeneity's sources and effects on experimental readouts and therapeutic efficacy. Among other topics, it demonstrates that heterogeneity exists in nearly all nanotherapeutic types, examines how nanotherapeutic heterogeneity arises, and discusses how heterogeneity impacts nanomaterials’ in vitro and in vivo behavior. How nanotherapeutic heterogeneity skews experimental readouts and complicates their optimization and clinical translation is also shown. Lastly, strategies for limiting nanotherapeutic heterogeneity are reviewed and recommendations for developing more reproducible and effective nanotherapeutics provided.
Electrospun Cellulose Nanocrystals Reinforced Flexible Sensing Paper for Triboelectric Energy Harvesting and Dynamic Self‐Powered Tactile Perception
Authors: Niu Zhaoxuan , Wang Qingxiang , Lu Jiqing , Hu Yi , Huang Jiaqi , Zhao Wei , Liu Yanju , Long Yun‐Ze , Han Guangping 
Published: December 2023

AbstractThe technical synergy between flexible sensing paper and triboelectric nanogenerator (TENG) in the next stage of artificial intelligence Internet of Things engineering makes the development of intelligent sensing paper with triboelectric function very attractive. Therefore, it is extremely urgent to explore functional papers that are more suitable for triboelectric sensing. Here, a cellulose nanocrystals (CNCs) reinforced PVDF hybrid paper (CPHP) is developed by electrospinning technology. Benefitting from the unique effects of CNCs, CPHP forms a solid cross‐linked network among fibers and obtains a high‐strength (25 MPa) paper‐like state and high surface roughness. Meanwhile, CNCs also improve the triboelectrification effect of CPHP by assisting the PVDF matrix to form more electroactive phases (96% share) and a higher relative permittivity (17.9). The CPHP‐based TENG with single electrode configuration demonstrates good output performance (open‐circuit voltage of 116 V, short‐circuit current of 2.2 µA and power density of 91 mW m−2) and ultrahigh pressure‐sensitivity response (3.95 mV Pa−1), which endows CPHP with reliable power supply and sensing capability. More importantly, the CPHP‐based flexible self‐powered tactile sensor with TENG array exhibits multifunctional applications in imitation Morse code compilation, tactile track recognition, and game character control, showing great prospects in the intelligent inductive device and human–machine interaction.
Phase‐Modulated Elastic Properties of 2D Magnetic FeTe: Hexagonal and Tetragonal Polymorphs
Authors: Yu Yunfei , Cheng Mo , Tao Zicheng , Han Wuxiao , Du Guoshuai , Guo Yanfeng , Shi Jianping , Chen Yabin 
Published: December 2023

Abstract2D layered magnets, such as iron chalcogenides, have emerged these years as a new family of unconventional superconductors and provided the key insights to understand the phonon‐electron interaction and pairing mechanism. Their mechanical properties are of strategic importance for the potential applications in spintronics and optoelectronics. However, there is still a lack of efficient approach to tune the elastic modulus despite the extensive studies. Herein, the modulated elastic modulus of 2D magnetic FeTe and its thickness‐dependence is reported via phase engineering. The grown 2D FeTe by chemical vapor deposition can present various polymorphs, that is tetragonal FeTe (t‐FeTe, antiferromagnetic) and hexagonal FeTe (h‐FeTe, ferromagnetic). The measured Young's modulus of t‐FeTe by nanoindentation method shows an obvious thickness‐dependence, from 290.9 ± 9.2 to 113.0 ± 8.7 GPa when the thicknesses increased from 13.2 to 42.5 nm, respectively. In comparison, the elastic modulus of h‐FeTe remains unchanged. These results can shed light on the efficient modulation of mechanical properties of 2D magnetic materials and pave the avenues for their practical applications in nanodevices.
Selective Detection of Functionalized Carbon Particles based on Polymer Semiconducting and Conducting Devices as Potential Particulate Matter Sensors
Authors: Song Yunjia , Chen Nan , Jiang Qifeng , Mukhopadhyay Tushita , Wondmagegn Wudyalew , Klausen Rebekka S. , Katz Howard E. 
Published: December 2023

AbstractThis paper reports a new mechanism for particulate matter detection and identification. Three types of carbon particles are synthesized with different functional groups to mimic the real particulates in atmospheric aerosol. After exposing polymer‐based organic devices in organic field effect transistor (OFET) architectures to the particle mist, the sensitivity and selectivity of the detection of different types of particles are shown by the current changes extracted from the transfer curves. The results indicate that the sensitivity of the devices is related to the structure and functional groups of the organic semiconducting layers, as well as the morphology. The predominant response is simulated by a model that yielded values of charge carrier density increase and charge carriers delivered per unit mass of particles. The research points out that polymer semiconductor devices have the ability to selectively detect particles with multiple functional groups, which reveals a future direction for selective detection of particulate matter.
Organic Photoredox Reactions in Two‐Molecule Photoredox System
Authors: Yoshimi Yasuharu 
Published: December 2023

AbstractUsing our recent relevant results, this account shows the featured reactivities of two‐molecule photoredox systems compared to one‐molecule photoredox systems. The low efficiency of electron transfer processes, such as photoinduced and back‐electron transfer, in the two‐molecule photoredox system, furnishes unique products through different pathways. The facile replacement of photoredox catalysts with appropriate oxidation/reduction potentials in this system provides valuable insights into photoredox reactions.