bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone marrow abnormalities on magnetic resonance imaging are associated with type II collagen degradation in knee osteoarthritis: A three‐month longitudinal study
Authors: Garnero P. , Peterfy C. , Zaim S. , Schoenharting M. 
Published: September 2005

AbstractObjectiveUsing radiography to assess the efficacy of a disease‐modifying osteoarthritis (OA) drug on joint structure is challenging. Subchondral bone marrow abnormalities determined by magnetic resonance imaging (MRI) and urinary excretion of C‐terminal crosslinking telopeptide of type II collagen (CTX‐II) have recently been shown to be predictors of radiographic progression in patients with knee OA, suggesting that these may represent valuable biomarkers with increased sensitivity compared with findings on radiography. The aims of this investigation were to analyze, in patients with knee OA, whether the values associated with these 2 OA biomarkers can change within 3 months, and to investigate the relationships between bone marrow abnormalities and CTX‐II.MethodsKnee MRI scans were obtained in 377 patients with painful knee OA (76% women, mean age 63 years, mean disease duration 6.6 years) at both baseline and 3 months. The femoral and tibial condyles and the patella were divided into 8 sites for the scoring of bone marrow abnormalities. A bone marrow abnormality was defined as an area of increased signal on T2‐weighted images of the subchondral bone. All scans were reviewed centrally and scored by a single trained radiologist using a validated 4‐point scoring method. Fasting urine and serum samples were also collected from all patients at baseline, month 1, month 2, and month 3, in order to measure the levels of urinary CTX‐II and serum CTX‐I, a biochemical marker of bone resorption.ResultsAt baseline, 82% of patients had MRI evidence of bone marrow abnormalities. Bone marrow abnormality scores correlated significantly with CTX‐II levels (P < 0.0001). Within 3 months, the bone marrow abnormality score decreased in 37 patients (9.8%), increased in 71 patients (18.8%), and did not change in the majority of patients (71.4%). Patients with baseline urinary CTX‐II levels in the highest tertile had a relative risk of 2.4 (95% confidence interval 1.1–5.0) of worsening bone marrow abnormalities at 3 months compared with patients with levels in the lowest tertile, after adjustment for age, sex, and body mass index. In patients who showed a decrease in the bone marrow abnormality score at 3 months, urinary CTX‐II levels decreased significantly (mean −75 ng/mmole creatinine), whereas levels increased (mean +23 ng/mmole creatinine) in patients showing an increase in the bone marrow abnormality score (P = 0.01 between the 2 groups). No significant association between bone marrow abnormalities and serum CTX‐I was observed.ConclusionIn patients with painful knee OA, bone marrow abnormalities on MRI can change within only 3 months in ∼30% of patients. Reduction in the extent of bone marrow abnormalities is associated with a decrease in cartilage degradation.
Who posts DeCSS and why?: A content analysis of Web sites posting DVD circumvention software
Authors: Eschenfelder Kristin R. , Glenn Howard Robert , Desai Anuj C. 
Published: September 2005

AbstractThis study explored why Web authors post the DVD decryption software known as DeCSS—specifically whether authors post DeCSS to protest changes in copyright law. Data are drawn from content analysis of Web sites posting the software. Most DeCSS posters did not include any content explaining why they posted DeCSS; however, no authors presented DeCSS as a piracy tool. Of sites containing explanatory content, many argued that DeCSS is a legitimate tool to play DVDs on free/open source computers. Other sites asserted that current copyright law is unjust, and that DVD‐related corporations are engaging in undesirable behaviors. Based on the data, and theorizing from rhetoric and the collective action literatures, we assert that much DeCSS posting is protest, but it may not be copyright protest—numerous posters protest related issues such as freedom of speech. More research is needed to determine the significance of DeCSS posting to broader copyright policy debates including its relation to off‐line protest, and the development of shared identities and cognitive frames. Also, the complexities of circumvention issues raise concerns about whether policy debate will be limited to elites. Finally, data point to the need to understand both international and local laws, norms, and events when studying copyright protest activity.
A validated chiral HPLC method for the determination of mebeverine HCl enantiomers in pharmaceutical dosage forms and spiked rat plasma
Authors: Radwan Mahasen A. , Abdine Heba H. , Aboul‐Enein Hassan Y. 
Published: September 2005

AbstractA new, precise, simple and accurate HPLC method was developed for the first time to separate and determine mebeverine enantiomers. Enantiomeric resolution was achieved on a cellulose Tris (3,5‐dimethylphenyl carbamate) column known as Chiralcel OD, with UV detection at 263 nm. The mobile phase consisted of n‐hexane, isopropyl alcohol and triethylamine (90:9.9:0.1 v/v/v). Sample run time was 18 min. On using the chromatographic conditions described, mebeverine enantiomers were well resolved with mean retention times of about 11 and 14 min. A linear response (r > 0.999) was observed over the concentration range 0.5–20 µg/mL racemic mebeverine. Precision, accuracy and stability were studied according to ICH guidelines. The limit of detection was found to be 0.05 µg/mL for each enantiomer of mebeverine. The proposed method was applied for analysis of mebeverine in commercially available tablets dosage formulations. Examples of application to biological samples are also given. Reanalysis of samples several weeks after the initial analysis showed no degradation of mebeverine. Copyright © 2005 John Wiley & Sons, Ltd.
Liquid chromatography with electrospray ionization mass spectrometry method for the assay of glucosamine sulfate in human plasma: validation and application to a pharmacokinetic study
Authors: Huang Tao‐Min , Cai Lei , Yang Bei , Zhou Man‐Xiang , Shen Yun‐Fei , Duan Geng‐Li 
Published: September 2005

AbstractA liquid chromatography–electrospray ionization mass spectrometry (LC–ESI–MS) method was developed and validated for the assay of glucosamine sulfate in human plasma. Plasma proteins were precipitated by acetonitrile, followed by vortex mixing and centrifugation. The supernatant was transferred and derivatized with phenyl iso‐thiocyanate in acetonitrile at 60°C for 40 min. Chromatographic separation was performed on a C18 column (Inertsil ODS‐3 150 × 2.1 mm i.d., 5 µm, JP) with a mobile phase gradient consisting of 0.2% acetic acid (aqueous) and methanol at a flow‐rate of 0.3 mL/min. MS detection using electrospray ionization (ESI) as an interface was used in single ion monitoring mode to determine positive ions at m/z 297. This method was shown to be selective and sensitive for glucosamine sulfate. The limit of detection was 35 ng/mL for glucosamine sulfate in plasma and the linear range was 0.1–20 µg/mL in plasma with a correlation coefficient (r) of 0.9991. The relative standard deviations (RSDs) of intra‐day and inter‐day assays were 8.7–11.4 and 9.8–12.6%, respectively. Extraction recoveries of glucosamine sulfate in plasma were greater than 73%. This method proved to be simple, reproducible and feasible for pharmacokinetic studies of glucosamine sulfate in healthy volunteers after a single oral administration (1500 mg). The pharmacokinetic parameters and relative bioavailabilities were investigated for both domestic glucosamine sulfate tablet and capsule preparations compared with an imported capsule product. Copyright © 2005 John Wiley & Sons, Ltd.
Diastereoselective Reactions at Enantiomerically Pure, Sterically Congested Cyclohexanes as an Entry to Wailupemycins A and B: Total Synthesis of (+)‐Wailupemycin B
Authors: Kirsch Stefan F. , Bach Thorsten 
Published: September 2005

AbstractWailupemycin A (1) and B (2) are polyketide natural products with a highly substituted cyclohexanone core. Three different routes for the syntheses of these compounds were pursued, which commenced from either (R)‐(−)‐carvone (ent‐5) or (S)‐(+)‐carvone (5). In the first approach it was attempted to construct the skeleton of wailupemycin A from triol 19 (nine steps from ent‐5; 19 % yield) by a sequence of diastereoselective epoxidation, nucleophilic ring opening at C‐13 and carbonyl addition at C‐5. The synthetic plan failed at the stage of the carbonyl addition to aldehyde 27, which had been obtained in seven steps (18 % yield) from triol 19. The second route included an epoxide ring opening at C‐13 and a carbonyl addition at C‐7 as key steps. It could have led to either wailupemycin A or B depending on the diastereoselectivity of the addition step. Starting from allylic alcohol 30 (six steps from ent‐5; 59 % yield) the cyclohexanone 28 was obtained in five steps (54 % yield). Unfortunately, the carbonyl addition failed also in this instance. In the eventually successful third attempt the skeleton of wailupemycin B was built from cyclohexanone 43 (eight steps from 5; 53 % yield) by highly diastereoselective carbonyl addition reactions at C‐7 and C‐12. The phenyl group at C‐14 was introduced at a late stage of the synthetic sequence. Careful protecting group manipulation finally allowed for the total synthesis of (+)‐wailupemycin B. The absolute and relative configuration of the natural product was unambiguously confirmed. The total yield of wailupemycin B amounted to 6 % over 23 steps starting from (S)‐(+)‐carvone (5).
Isothermal Section of the Cu2Se—HgSe—GeSe2 System at 670 K and Crystal Structures of the Compounds Cu2HgGeSe4 and HT‐Modification of Cu2HgGeS4.
Authors: Olekseyuk I. D. , Marchuk O. V. , Gulay L. D. , Zhbankov O. Ye. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nanostructuring, Compositional Fluctuations, and Atomic Ordering in the Thermoelectric Materials AgPbmSbTe2+m. The Myth of Solid Solutions.
Authors: Quarez Eric , Hsu Kuei‐Fang , Pcionek Robert , Frangis N. , Polychroniadis E. K. , Kanatzidis Mercouri G. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Crystal Structures, Electronic Properties, and Pressure‐Induced Superconductivity of the Tetrahedral Cluster Compounds GaNb4S8, GaNb4Se8, and GaTa4Se8.
Authors: Pocha Regina , Johrendt Dirk , Ni Bingfang , Abd‐Elmeguid Mohsen M. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Photochemical Conversion of N2O into N2 and O2 by 193‐nm‐ArF‐Excimer Laster in N2 or Air at Atmospheric Pressure.
Authors: Tsuji Masaharu , Sako Hiroshi , Noda Kenji , Senda Makoto , Hamagami Taro , Tsuji Takeshi 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Layered Fluorinated Gallium Phosphate Organically Templated by Propane‐1,3‐diaminium, an Analogue of the Aluminophosphate MIL‐12: Ga2 (PO4) F5×C3H12N2.
Authors: Loiseau Thierry , Ferey Gerard 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
([2]Paracyclo[2](5,8)quinolinophan‐2‐yl)carbinols as Catalysts for Diethylzinc Addition to Aldehydes: Cooperative Effects of Planar and Central Chirality on the Asymmetric Induction.
Authors: Ricci Giacomo , Ruzziconi Renzo 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Enantioselective Alkynylations of Aromatic and Aliphatic Aldehydes Catalyzed by Terpene Derived Chiral Amino Alcohols.
Authors: Watts Cian Christopher , Thoniyot Praveen , Hirayama Lacie C. , Romano Talia , Singaram Bakthan 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Polymer‐Supported Chiral Sulfonamide Catalyzed One‐Pot Reduction of β‐Keto Nitriles: A Practical Synthesis of (R)‐Fluoxetine and (R)‐Duloxetine.
Authors: Wang Guangyin , Liu Xingshun , Zhao Gang 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
New Chiral Bis(diphenylphospholane) Ligands: Design, Synthesis, and Application to Catalytic Enantioselective Aldol Reaction to Ketones.
Authors: Oisaki Kounosuke , Zhao Dongbo , Suto Yutaka , Kanai Motomu , Shibasaki Masakatsu 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Michael Reaction of 1,3‐Dicarbonyls with Enones Catalyzed by a Hydroxyapatite‐Bound La Complex.
Authors: Mori Kohsuke , Oshiba Michitaka , Hara Takayoshi , Mizugaki Tomoo , Ebitani Kohki , Kaneda Kiyotomi 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient and Mild Oxidative Nuclear Thiocyanation of Activated Aromatic Compounds Using Ammonium Thiocyanate and Diacetoxyiodobenzene.
Authors: Karade N. N. , Tiwari G. B. , Shirodkar S. G. , Dhoot B. M. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Facile Preparation of Various Heteroaromatic Compounds via Azatitanacyclopentadiene Intermediates.
Authors: Suzuki Daisuke , Nobe Youhei , Watai Yuko , Tanaka Ryoichi , Takayama Yuuki , Sato Fumie , Urabe Hirokazu 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Electrosynthesis of 2‐Arylpropionic Acids from α‐Methylbenzyl Chlorides and Carbon Dioxide by [Co(Salen)].
Authors: Damodar J. , Mohan S. Krishna , Lateef S. K. Khaja , Reddy S. Jayarama 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
syn‐π‐Face‐ and endo‐Selective, Inverse Electron‐Demand Diels—Alder Reactions of 3,4‐Di‐tert‐butylthiophene 1‐Oxide with Electron‐Rich Dienophiles.
Authors: Takayama Jun , Sugihara Yoshiaki , Takayanagi Toshiyuki , Nakayama Juzo 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A C—C Bond Formation Reaction at the α‐Carbon Atom of α‐Oxo Ketene Dithioacetals via the Baylis—Hillman Type Reaction.
Authors: Yin Yan‐Bing , Wang Mang , Liu Qun , Hu Jiang‐Lei , Sun Shao‐Guang , Kang Jing 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Mn(III)‐Promoted Cyclization of Substituted Thioformanilides under Microwave Irradiation: A New Reagent for 2‐Substituted Benzothiazoles.
Authors: Mu Xue‐Jun , Zou Jian‐Ping , Zeng Run‐Sheng , Wu Jun‐Chen 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactivity of 3,5‐Bis‐(4‐amino‐1,2,5‐oxadiazol‐3‐yl)‐1,2,4‐triazole.
Authors: Sergievskii A. V. , Romanova T. V. , Mel'nikova S. F. , Tselinskii I. V. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Evolution of the Pseudo‐1,3‐dipolar Cycloaddition Chemistry of SNSMF6 (M: As, Sb) Leading to 2,5‐Dihydroxybenzo‐1,3,2‐dithiazolylium and 2,7‐Dicarbonylnaphtha‐1,3,2‐dithiazolylium Salts and Their Corresponding Radicals.
Authors: Decken Andreas , Mailman Aaron , Mattar Saba M. , Passmore Jack 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Chromane Derivatives by Palladium‐Catalyzed Intramolecular Allylation of Aldehydes with Allylic Acetates or Chlorides Using Indium and Indium(III) Chloride.
Authors: Nguyen Van Cuong , Kim Yong‐Tae , Yu Yeon‐Kwon , Kang Han‐Young 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Microwave‐Assisted Multistep Synthesis of Functionalized 4‐Arylquinolin‐2(1H)‐ones Using Palladium‐Catalyzed Cross‐Coupling Chemistry.
Authors: Glasnov Toma N. , Stadlbauer Wolfgang , Kappe C. Oliver 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Tricyclic Analogues of Stephaoxocanidine and Their Evaluation as Acetylcholinesterase Inhibitors.
Authors: Bianchi Dario A. , Hirschmann Guillermo Schmeda , Theoduloz Cristina , Bracca Andrea B. J. , Kaufman Teodoro S. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Heterocyclic Synthesis with 4‐Hydrazinopyridothienopyrimidines: Synthesis of Pyridothienotriazolopyrimidines and Heterocyclylpyridothienopyrimidines with Biological Interest.
Authors: Gaber Hatem M. , Elgemeie Galal E. H. , Ouf Salama A. , Sherif Sherif M. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Investigation of the Products of Interaction of Cyclic Diketones with Nitrogen‐Containing 1,4‐Binucleophiles.
Authors: Kolos N. N. , Yurchenko E. N. , Orlov V. D. , Shishkina S. V. , Shishkin O. V. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel 2‐N,N‐Dimethylaminomethyl‐2,3,3a,12b‐tetrahydrodibenzo[b,f]furo [2,3‐d]oxepin Derivatives Displaying Combined Norepinephrine Reuptake Inhibition and 5‐HT2A/2C Receptor Antagonism.
Authors: Bartolome Jose M. , Alcudia Ana , Andres Jose I. , Cid Jose M. , Garcia Mercedes , Megens Anton , Toledo Miguel A. , Trabanco Andres A. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A New Tomato Pregnane Glycoside from the Overripe Fruits
Authors: Fujiwara Yukio , Yoshizaki Miho , Matsushita Sayaka , Yahara Shoji , Yae Emiko , Ikeda Tsuyoshi , Ono Masateru , Nohara Toshihiro 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
2,4,5‐Trichlorophenyl‐ (9H‐fluoren‐9‐ylmethoxycarbonylamino)methylcarbamates: Synthesis, Isolation, Characterization and Utility in the Synthesis of Dipeptidyl Ureas.
Authors: Babu Vommina V. Suresh , Kantharaju Kantharaju 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Preparation of 2,3‐Diaminopropionate from Ring Opening of Aziridine‐2‐carboxylate.
Authors: Kim Yongeun , Ha Hyun‐Joon , Han Kyusung , Ko Seung Whan , Yun Hoseop , Yoon Hyo Jae , Kim Min Sung , Lee Won Koo 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Novel Conformationally Locked Carbocyclic Nucleosides Derived from 5,5‐ and 6,6‐Bis(hydroxymethyl)bicyclo[2.2.1]heptan‐2‐ol.
Authors: Hrebabecky Hubert , Masojidkova Milena , Holy Antonin 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Design, Synthesis and Structure—Activity Relationships of 6‐O‐Arylpropargyl Diazalides with Potent Activity Against Multidrug‐Resistant Streptococcus Pneumoniae.
Authors: Yong Hong , Gu Yu Gui , Clark Richard F. , Marron Thomas , Ma Zhenkun , Soni Niru , Stone Gregory G. , Nilius Angela M. , Marsh Kennan , Djuric Stevan W. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereochemistry of Phellinsin A: A Concise Synthesis of α‐Arylidene‐γ‐Lactones.
Authors: Kim Eungsoo , Lee Hyeong Kyu , Hwang Eui‐Il , Kim Sung‐Uk , Lee Woo Song , Lee Sangku , Jung Sang‐Hun 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Metallaborane Reaction Chemistry. Part 12. Some Interactions of Acetylenes and Isocyanides with Selected Metallaboranes
Authors: Bould Jonathan , Bown Mark , Coldicott Richard J. , Ditzel Evert J. , Greenwood Norman N. , Macpherson Ian , MacKinnon Peter , Thornton‐Pett Mark , Kennedy John D. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Organic Chemistry of Subvalent Transition Metal Complexes. Part 33. The Decomposition of Transition Metal Alkyls Revisited: Surprising Wellspring of Novel Reagents for Organic Synthesis
Authors: Eisch John J. , Adeosun Adetenu A. , Dutta Somnath , Fregene Paul O. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nicastrin controls aspects of photoreceptor neuron specification and differentiation in the Drosophila eye
Authors: Zhang Tianyi , Tran Susan , Clouser Christopher , Pignoni Francesca 
Published: September 2005

AbstractNicastrin is a component of the Notch signaling pathway involved in proteolytic release of the Notch receptor intracellular domain. It has been postulated that intracellular Notch is required within the nucleus of fly eye progenitor cells to enhance (proneural enhancement) and then repress (lateral inhibition) transcription of proneural genes. We present here an analysis of Nicastrin function during eye development and find that Nicastrin is essential to early photoreceptor neuron development. Nicastrin mutant tissue displays neuronal loss or hyperplasia; these phenotypes can be rescued by targeted expression of an intracellular form of Notch. Thus, nuclear translocation of Notch and its direct regulation of gene expression appear to be critical to proneural enhancement as well as lateral inhibition. In addition, we show that Nicastrin as well as Notch are required to maintain normal R‐cell morphology, because the nuclei of mutant photoreceptor neurons cannot maintain their proper position. Thus, Notch signaling plays a role, not only in cell fate specification, but also in differentiation of photoreceptor neurons. Developmental Dynamics 234:590–601, 2005. © 2005 Wiley‐Liss, Inc.
The basic helix‐loop‐helix factor Hand2 regulates autonomic nervous system development
Authors: Morikawa Yuka , Dai Yan‐Shan , Hao Jianming , Bonin Christopher , Hwang Sunny , Cserjesi Peter 
Published: September 2005

AbstractMammalian autonomic nervous system (ANS) development requires the combinatorial action of a number of transcription factors, which include Mash1, Phox2b, and GATA3. Here we show that the bHLH transcription factor, Hand2 (dHAND), is expressed concurrently with Mash1 during sympathetic nervous system (SNS) development and that the expression of Hand2 is not dependent on Mash1. This suggests that these two bHLH factors work in parallel during SNS development. We also show that ectopic expression of Hand2 activates the neuronal program and promotes the acquisition of a phenotype corresponding to peripheral neurons including neurons of the SNS lineage in P19 embryonic carcinoma cells. We propose that Hand2 works in parallel with other members of the transcriptional network to regulate ANS developmental but can ectopically activate the program by a cross‐regulatory mechanism that includes the activation of Mash1. We show that this function is dependent on its interaction with the histone acetyltransferase p300/CBP, indicating that Hand2 functions to promote ANS development as part of a larger transcriptional complex. Developmental Dynamics 234:613–621, 2005. © 2005 Wiley‐Liss, Inc.
Short‐term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord
Authors: Ding Lei , Takebayashi Hirohide , Watanabe Keisuke , Ohtsuki Toshiaki , Tanaka Kenji F. , Nabeshima Yo‐ichi , Chisaka Osamu , Ikenaka Kazuhiro , Ono Katsuhiko 
Published: September 2005

AbstractWe examined the migration and differentiation of cells expressing Olig3, a basic helix‐loop‐helix transcriptional factor, in the developing spinal cord. Distribution of Olig3 lineage cells was demonstrated with in situ hybridization and X‐gal staining in an Olig3‐lacZ knock‐in mouse. Olig3‐positive cells first appeared in the dorsal spinal cord, except for the roof plate. Some of the dorsal Olig3 lineage cells co‐expressed Islet1/2, Math1, or Brn3a, markers for dorsal interneuron. LacZ‐positive cells were observed in the ventral‐most part of the E10.5 spinal cord, suggesting that some dorsal Olig3 lineage cells migrate into the ventral‐most part by E10.5. Ventral‐ward migration of dorsal cells and contribution to commissural interneurons were substantiated by electroporation of EGFP expression plasmid in the dorsal spinal cord of chick embryo. Dorsal midline cells were also LacZ‐positive during development. These findings suggest that dorsal Olig3 cells contribute to dorsal midline cells and commissural interneurons at intermediate and ventral levels. Developmental Dynamics 234:622–632, 2005. © 2005 Wiley‐Liss, Inc.