bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementing knowledge management solutions in a public research institution: the CNRS case
Authors: Lelièvre Gérard , Souillot Jacques 
Published: May 2005

AbstractThe Mission des Ressources et Compétences Technologiques (MRCT), of the Centre National de la Recherche Scientifique (CNRS), is a small unit in charge of optimizing the technological potential of the institution. One of its main objectives is to provide the CNRS scientific research teams with the best technological conditions that can be hoped for on the basis of the tools, competencies, structures, and funds that are available throughout the institution. Redistributing, or even sharing, elements of all natures, whatever the situation, is always a task demanding heavy efforts in the public sector. The MRCT has developed a soft approach to make things evolve as smoothly as possible and take into account the reactivity of the knowledge eco‐system it is inviting to transform. Extra carefulness, and realism, has driven the MRCT team to implement its knowledge management approach by impregnation, i.e. via its competencies networks. Copyright © 2005 John Wiley & Sons, Ltd.
Feature selection methods involving support vector machines for prediction of insolvency in non‐life insurance companies
Authors: Salcedo‐Sanz Sancho , DePrado‐Cumplido Mario , Segovia‐Vargas María Jesús , Pérez‐Cruz Fernando , Bousoño‐Calzón Carlos 
Published: May 2005

AbstractWe propose two novel approaches for feature selection and ranking tasks based on simulated annealing (SA) and Walsh analysis, which use a support vector machine as an underlying classifier. These approaches are inspired by one of the key problems in the insurance sector: predicting the insolvency of a non‐life insurance company. This prediction is based on accounting ratios, which measure the health of the companies. The approaches proposed provide a set of ratios (the SA approach) and a ranking of the ratios (the Walsh analysis ranking) that would allow a decision about the financial state of each company studied. The proposed feature selection methods are applied to the prediction the insolvency of several Spanish non‐life insurance companies, yielding state‐of‐the‐art results in the tests performed. Copyright © 2005 John Wiley & Sons, Ltd.
Expression pattern of the chromosome 21 transcription factor Ets2 in cell‐seeded three‐dimensional bone constructs
Authors: Turhani Dritan , Watzinger Elisabeth , Weißenböck Martina , Yerit Kaan , Cvikl Barbara , Ewers Rolf , Thurnher Dietmar 
Published: May 2005

AbstractThe ability to generate new bone for reconstructive surgery use is a major clinical need. Tissue engineering with osteoprogenitor cells isolated from the patient's periosteum and seeded into bioresorbable scaffolds offers a promising approach to the generation of skeletal tissue. To our knowledge, there is no description about the expression of Ets2 in tissue engineered “bone neotissue.” The aim of our study was to manufacture cell‐seeded three‐dimensional bone constructs with human periosteal cells on poly (lactic‐co‐glycolic acid) polymer fleeces to describe the expression pattern of Ets2 and its target genes osteocalcin and osteopontin; expression analysis of type I collagen, core‐binding factor‐1, alkaline phosphatase, and osteonectin; the ability of matrix mineralization and ALP enzymatic activity showed the osteogenic character of the constructs. A significant correlation between the expression of Ets2 and osteopontin mRNA (r = −0.70; p < 0.05) could be shown. A 1.35‐fold increase of Ets2 expression from days 1 to 9 was detected, followed by a slight decrease from days 11 to 15. Until the end of the culture period, the expression of Ets2 reached a comparable high level as detected on day 9. In contrast, the expression level of osteopontin mRNA reached a maximum at day 7, followed by a progressive 3.04‐fold decrease until day 21. This study shows for the first time that Ets2 gene and its transcriptional target genes are expressed in tissue‐engineered bone constructs. These findings have the potential to provide much‐needed information about the role and function of Ets2 in human osteogenesis processes and creation of “bone neotissue.” © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Development of new injectable bulking agents: Biocompatibility of radiopaque polymeric microspheres studied in a mouse model
Authors: Emans Pieter J. , Saralidze Ketie , Knetsch Menno L. W. , Gijbels Marion J. J. , Kuijer Roel , Koole Leo H. 
Published: May 2005

AbstractRadiopaque polymeric microspheres have a potential as new bulking agents for treatment of stress urinary incontinence (SUI). The advantage over existing bulking agents lies in their X‐ray visibility in situ; other polymeric bulking agents (e.g., PTFE or silicone rubbers) are practically radiolucent (i.e., incapable of absorbing X‐radiation). Radiopacity is useful in practice because of the high spatial accuracy of X‐ray imaging. For instance, X‐ray fluoroscopy can be used to assess possible migration of the bulking agent over time or to provide guidance in cases in which a second injection of a bulking agent is necessary (repeated treatment of SUI). Biocompatibility of injected radiopaque microspheres was investigated in vivo by using the mouse as a model. Microspheres were injected subcutaneously (9 animals) or intramuscularly (9 animals), and follow‐up was 8 days or 3 months. X‐ray fluoroscopy gave clear images of the miscrospheres as an ensemble, and it was found that no migration occurred during 3 months. Histopathology confirmed that all microspheres stayed close to the site of the injection. The microspheres appeared to be well tolerated; only a few giant cells, manifesting a mild inflammatory reaction, were encountered. At 3 months, cappillary blood vessels were observed throughout the microsphere beds, and macrophages and fibroblast cells were seen in between the microspheres. This is encouraging with respect to the intended application, although it must be acknowledged that the data refer merely to a mouse model. Further experiments with larger, more representative models (rabbit and goat) are in progress. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Novel functional biodegradable polymer. III. The construction of poly(γ‐glutamic acid)‐sulfonate hydrogel with fibroblast growth factor‐2 activity
Authors: Matsusaki Michiya , Serizawa Takeshi , Kishida Akio , Akashi Mitsuru 
Published: May 2005

AbstractPoly(γ‐glutamic acid)‐sulfonate (γ‐PGA‐S) hydrogel, with fibroblast growth factor (FGF)‐2 activity was investigated as a novel, next‐generation tissue‐engineering material. γ‐PGA‐net‐γ‐PGA‐S72 (S72‐netgel) was prepared with γ‐PGA‐S and γ‐PGA‐S72 (72% sulfonated γ‐PGA) to provide the high mobility of γ‐PGA‐S72 for FGF‐2 activity. Cell adhesion and proliferation activities were evaluated on γ‐PGA and γ‐PGA‐S hydrogels along with S72‐netgels. Both cell adhesion and proliferation activities of γ‐PGA and γ‐PGA‐S hydrogels were low. In contrast, S72‐netgels had high cell adhesion and proliferation activities, because of their low swelling ratios and high sulfonic acid group concentrations. Furthermore, S72‐netgels had high FGF‐2 activity, because γ‐PGA‐S72 retained FGF‐2 activity when incorporated into S72‐netgels. S72‐netgels should be useful as next‐generation tissue‐engineering material containing FGF‐2 activity. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Evaluation of modifying collagen matrix with RGD peptide through periodate oxidation
Authors: Zhang Lihai , Hum Min , Wang Meng , Li Yanfeng , Chen Hua , Chu Chengbin , Jiang Hua 
Published: May 2005

AbstractThe aim of the study is to evaluate the effect of modifying collagen matrices with Arg‐Gly‐Asp (RGD) peptide through periodate oxidation. The collagen matrices were modified with RGD peptide, by periodate activation. The modified collagen matrices and unmodified matrices were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and electron spectroscopy for chemical analysis (ESCA). Mesenchymal stem cells (MSCs) were used to evaluate the cell compatibility of collagen matrices. In terms of cell growth, the MSCs attached much better on the modified matrix than on the unmodified one. But there was no significant difference between two groups regarding the MSC proliferation. Compared to the unmodified matrices, the mechanical strength of the modified matrix decreased sharply, and its 3D structure was destroyed. Introducing specific RGD receptor‐mediated adhesion sites on matrices obviously enhanced the MSC adhesion on collagen matrices, but the coupled method of periodate oxidation would likely result in the declination of the mechanical strength of the matrix, as well as the destruction of the matrix structure. This would affect the cell growth on the matrix, and decrease the histocompatibility of the matrices. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
Effect of synovial fluid, phosphate‐buffered saline solution, and water on the dissolution and corrosion properties of CoCrMo alloys as used in orthopedic implants
Authors: Lewis A. C. , Kilburn M. R. , Papageorgiou I. , Allen G. C. , Case C. P. 
Published: May 2005

AbstractThe corrosion and dissolution of high‐ and low‐carbon CoCrMo alloys, as used in orthopedic joint replacements, were studied by immersing samples in phosphate‐buffered saline (PBS), water, and synovial fluid at 37°C for up to 35 days. Bulk properties were analyzed with a fine ion beam microscope. Surface analyses by X‐ray photoelectron spectroscopy and Auger electron spectroscopy showed surprisingly that synovial fluid produced a thin oxide/hydroxide layer. Release of ions into solution from the alloy also followed an unexpected pattern where synovial fluid, of all the samples, had the highest Cr concentration but the lowest Co concentration. The presence of carbide inclusions in the alloy did not affect the corrosion or the dissolution mechanisms, although the carbides were a significant feature on the metal surface. Only one mechanism was recognized as controlling the thickness of the oxide/hydroxide interface. The analysis of the dissolved metal showed two mechanisms at work: (1) a protein film caused ligand‐induced dissolution, increasing the Cr concentration in synovial fluid, and was explained by the equilibrium constants; (2) corrosion at the interface increased the Co in PBS. The effect of prepassivating the samples (ASTM F‐86‐01) did not always have the desired effect of reducing dissolution. The release of Cr into PBS increased after prepassivation. The metal–synovial fluid interface did not contain calcium phosphate as a deposit, typically found where samples are exposed to calcium rich bodily fluids. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
A combined freeze‐and‐cut strategy for the description of large molecular systems using a localized orbitals approach
Authors: Borini Stefano , Maynau Daniel , Evangelisti Stefano 
Published: May 2005

AbstractA technique to reduce the computational effort in calculating ab initio energies using a localized orbitals approach is presented. By exploiting freeze strategy at the self‐consistent field (SCF) level and a cut of the unneeded atomic orbitals, it is possible to perform a localized complete active space (CAS‐SCF) calculation on a reduced system. This will open the possibility to perform ab initio treatments on very large molecular systems, provided that the chemically important phenomena happen in a localized zone of the molecule. Two test cases are discussed, to illustrate the performance of the method: the cis–trans interconversion curves for the (7Z)‐13 ammoniotridec‐7‐enoate, which demonstrates the ability of the method to reproduce the interactions between charged groups; and the cisoid–transoid energy barrier for the aldehydic group in the C13 polyenal molecule. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 1042–1051, 2005
Practical conversion from torsion space to Cartesian space for in silico protein synthesis
Authors: Parsons Jerod , Holmes J. Bradley , Rojas J. Maurice , Tsai Jerry , Strauss Charlie E. M. 
Published: May 2005

AbstractMany applications require a method for translating a large list of bond angles and bond lengths to precise atomic Cartesian coordinates. This simple but computationally consuming task occurs ubiquitously in modeling proteins, DNA, and other polymers as well as in many other fields such as robotics. To find an optimal method, algorithms can be compared by a number of operations, speed, intrinsic numerical stability, and parallelization. We discuss five established methods for growing a protein backbone by serial chain extension from bond angles and bond lengths. We introduce the Natural Extension Reference Frame (NeRF) method developed for Rosetta's chain extension subroutine, as well as an improved implementation. In comparison to traditional two‐step rotations, vector algebra, or Quaternion product algorithms, the NeRF algorithm is superior for this application: it requires 47% fewer floating point operations, demonstrates the best intrinsic numerical stability, and offers prospects for parallel processor acceleration. The NeRF formalism factors the mathematical operations of chain extension into two independent terms with orthogonal subsets of the dependent variables; the apparent irreducibility of these factors hint that the minimal operation set may have been identified. Benchmarks are made on Intel Pentium and Motorola PowerPC CPUs. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 1063–1068, 2005
A CSOV study of the difference between HF and DFT intermolecular interaction energy values: The importance of the charge transfer contribution
Authors: Piquemal Jean‐Philip , Marquez Antonio , Parisel Olivier , Giessner–Prettre Claude 
Published: May 2005

AbstractIntermolecular interaction energy decompositions using the Constrained Space Orbital Variation (CSOV) method are carried out at the Hartree–Fock level on the one hand and using DFT with usual GGA functionals on the other for a number of model complexes to analyze the role of electron correlation in the intermolecular stabilization energy. In addition to the overall stabilization, the results provide information on the variation, with respect to the computational level, of the different contributions to the interaction energy. The complexes studied are the water linear dimer, the N‐methylformamide dimer, the nucleic acid base pairs, the benzene–methane and benzene‐N2 van der Waals complexes, [Cu+‐(ImH)3]2, where “ImH” stands for the Imidazole ligand, and ImH‐Zn++. The variation of the frozen core energy (the sum of the intermolecular electrostatic energy and the Pauli repulsion energy) calculated from the unperturbed orbitals of the interacting entities indicates that the intramolecular correlation contributions can be stabilizing as well as destabilizing, and that general trends can be derived from the results obtained using usual density functionals. The most important difference between the values obtained from HF and DFT computations concerns the charge transfer contribution, which, in most cases, undergoes the largest increase. The physical meaning of these results is discussed. The present work gives reference calculations that might be used to parametrize new correlated molecular mechanics potentials. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 1052–1062, 2005
Interleukin‐4 gene, but not the interleukin‐1 beta gene polymorphism, is associated with oral cancer
Authors: Tsai Ming‐Hsui , Chen Wen‐Chi , Tsai Chang‐Hai , Hang Liang‐Wen , Tsai Fuu‐Jen 
Published: May 2005

AbstractWe aimed to evaluate whether polymorphisms of the interleukin‐4 (IL‐4) gene promoter and intron 3 regions, and polymorphisms of the IL‐1 beta gene promoter and exon 5 regions are associated with oral cancer. This study included 130 patients with oral cancer and 105 age‐matched healthy controls who lived in the same area as the patients. Each genetic polymorphism was typed by polymerase chain reaction (PCR)‐based restriction analysis. We then compared the genotype distribution and allelic frequencies of each polymorphism between the oral cancer patients and the controls. The CC homozygote genotype of the IL‐4 gene promoter −590 region differed significantly between the patients with oral cancer and the controls (odds ratio (OR)=6.0, 95% confidence interval (CI)=1.2−30.7, chi‐square test, P=0.044). No significant difference in either the genotype distribution or the allelic frequencies of the IL‐1 beta gene polymorphisms was observed between patients with oral cancer and controls. The IL‐4 gene −590 C/T polymorphism is associated with oral cancer and is a suitable genetic marker for screening for oral cancer. However, whether the −590 C/T polymorphism of the IL4 gene plays a role in oral cancer remains unclear. Further substantiation based on larger patient samples is needed. J. Clin. Lab. Anal. 19:93–98, 2005. © 2005 Wiley‐Liss, Inc.
Image analysis systems for the detection of disseminated breast cancer cells on bone‐marrow cytospins
Authors: Becker Sven , Becker‐Pergola Graziella , Fehm Tanja , Emig Robert , Wallwiener Diethelm , Solomayer Erich‐Franz 
Published: May 2005

AbstractWe assessed the accuracy of automated cell imaging systems when compared to manual evaluation of cytospin slides in determining the presence of cytokeratin‐positive, disseminated breast cancer cells in bone marrow aspirates. A total of 298 cytospin slides of bone marrow aspirates were first evaluated by individual screening by one expert immunocytologist. Subsequently, all 298 slides were evaluated by the Automated Cell Imaging System (ACIS) by ChromaVision™. Two separate analysis cycles were performed using ACIS. The results of the two ACIS analysis cycles were almost identical: in 293 out of 298 samples (98.3%), identical numbers of disseminated breast cancer cells were detected. In the remaining five samples (1.7%), the result of the two ACIS analysis cycles differed by only one tumor cell. By using the manual technique, 120 cytospin samples were found to be positive. ACIS was able to detect additional tumor cells in 64 cases. Not once did ACIS miss tumor cells when compared to the manual technique. Using ACIS, we were able to determine the bone marrow status of patients with nonmetastatic breast cancer faster, with greater accuracy, and with greater reproducibility than with the manual technique. J. Clin. Lab. Anal. 19:115–119, 2005. © 2005 Wiley‐Liss, Inc.
Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G
Authors: Gasparyan Vardan K. 
Published: May 2005

AbstractConjugation of immunoglobulin G (IgG) from rabbit serum with colloidal gold for sol agglutination assay was conducted. Effects of various buffer systems on conjugation and stability of sols were studied. Colloidal gold assay for detection of human myoglobin and C‐reactive protein was developed. It was shown that rabbit IgG had a poor stabilizing effect on colloidal gold sol and therefore that it cannot be effectively used in such assays. In contrast, hen egg immunoglobulin Y (IgY) had excellent stabilizing properties used in various buffer systems and in a wide range of concentrations. IgY was considered as a useful alternative for rabbit antibodies in gold sol agglutination assay. J. Clin. Lab. Anal. 19:124–127, 2005. © 2005 Wiley‐Liss, Inc.
In vivo cutaneous interferon‐γ gene delivery using novel dicationic (gemini) surfactant–plasmid complexes
Authors: Badea Ildiko , Verrall Ronald , Baca‐Estrada Maria , Tikoo Suresh , Rosenberg Alan , Kumar Praveen , Foldvari Marianna 
Published: May 2005

AbstractBackgroundLocalized scleroderma (morphea and linear scleroderma) is a connective tissue disease, accompanied by excessive proliferation and deposition of collagen within the skin, inflammation, vasculopathy and a deranged immune system. Interferon γ (IFNγ), an inhibitor of collagen synthesis and an immunomodulator, could be a potential therapeutic agent if it could be delivered into or expressed locally in affected skin in a non‐invasive manner. In this study, the feasibility of topical delivery of the IFNγ gene and expression of IFNγ were investigated in mice.MethodsNovel dicationic (gemini) surfactant (spacer length n = 2–16; alkyl chain m = 12 or 16)–DNA complexes were formulated and characterized by circular dichroism and atomic force microscopy to select gemini analogues with the highest transfection efficiency (TE). Transfection and cellular expression of IFNγ from the bicistronic pGTmCMV.IFN‐GFP plasmid were evaluated in PAM 212 keratinocyte culture by ELISA and fluorescence microscopy. Topical delivery of plasmid using liposomal and nanoemulsion systems, based on gemini surfactant 16‐3‐16, was evaluated in mice by IFNγ expression analysis.ResultsIn vitro TE was found to be dependent on the spacer length of the gemini surfactant, with the C3 spacer showing the highest activity (both 12‐3‐12 and 16‐3‐16). Both gemini cationic liposomes and gemini nanoemulsion (3 × 25 µg DNA/animal) produced significantly higher levels of IFNγ in the skin (359.4 and 607.24 pg/cm2) compared to naked DNA (135.69 pg/cm2) or a liposomal Dc‐chol formulation (82.15 pg/cm2). IFNγ expression in the lymph nodes was higher in the animals treated with gemini liposomes (422.74 pg/animal) compared to the nanoemulsion formulation (131.27 pg/animal) or the Dc‐chol formulation (82pg/animal).ConclusionsThe feasibility of topical delivery of pGTmCMV.IFN‐GFP plasmid in mice using gemini cationic surfactant based delivery systems was demonstrated. IFNγ expression after treatment with gemini–DNA formulations in the skin was 3–5‐fold higher compared to the treatment with naked DNA (p < 0.05), and 4–6‐fold higher than the Dc‐chol–DNA complex, indicating a significant advance in topical DNA delivery across intact skin in vivo. Copyright © 2005 John Wiley & Sons, Ltd.
Raman scattering study of adsorption/desorption of water from single‐walled carbon nanotubes
Authors: Sharma Suresh C. , Singh Dharmbir , Li Ying 
Published: May 2005

AbstractWe utilized Raman spectroscopy to investigate H2O adsorption in and desorption from single‐walled carbon nanotubes (SWCNTs). SWCNT tips were opened by known chemical methods and H2O was inserted into the tubes via capillary action over periods up to 136 h. The wavenumbers in the OH bending region of H2O increase almost exponentially with H2O treatment time. Following H2O adsorption for 136 h, Raman spectra were also measured as a function of heat‐treatment temperature. These data lead to 0.07 eV for the H2O desorption energy from SWCNTs. Copyright © 2005 John Wiley & Sons, Ltd.