bluuebunny/crossref_metadata_2025_split huggingface dataset:
The anti-platelet drug cilostazol enhances heart rate and interrenal steroidogenesis and exerts a scant effect on innate immune responses in zebrafish
Authors: Chang Wei-Chun , Chen Mei-Jen , Hsiao Chung-Der , Hu Rong-Ze , Huang Yu-Shan , Chen Yu-Fu , Yang Tsai-Hua , Tsai Guan-Yi , Chou Chih-Wei , Chen Ren-Shiang , Chuang Yung-Jen , Liu Yi-Wen 
Published: October 2023

Rationale
Cilostazol, an anti-platelet phosphodiesterase-3 inhibitor used for the treatment of intermittent claudication, is known for its pleiotropic effects on platelets, endothelial cells and smooth muscle cells. However, how cilostazol impacts the endocrine system and the injury-induced inflammatory processes remains unclear.

Methods
We used the zebrafish, a simple transparent model that demonstrates rapid development and a strong regenerative ability, to test whether cilostazol influences heart rate, steroidogenesis, and the temporal and dosage effects of cilostazol on innate immune cells during tissue damage and repair.

Results
While dosages of cilostazol from 10 to 100 μM did not induce any noticeable morphological abnormality in the embryonic and larval zebrafish, the heart rate was increased as measured by ImageJ TSA method. Moreover, adrenal/interrenal steroidogenesis in larval zebrafish, analyzed by whole-mount 3β-Hsd enzymatic activity and cortisol ELISA assays, was significantly enhanced. During embryonic fin amputation and regeneration, cilostazol treatments led to a subtle yet significant effect on reducing the aggregation of Mpx-expressing neutrophil at the lesion site, but did not affect the immediate injury-induced recruitment and retention of Mpeg1-expressing macrophages.

Conclusions
Our results indicate that cilostazol has a significant effect on the heart rate and the growth as well as endocrine function of steroidogenic tissue; with a limited effect on the migration of innate immune cells during tissue damage and repair.
Virtual faculty development program in bioethics evaluated by Kirkpatrick model: A unique opportunity
Authors: Shallal Tara M. A. , Alkhateeb Nazdar Ezzaddin , Al-Dabbagh Ali 
Published: October 2023

Background
With a shortage of teachers willing to deliver bioethics courses, particularly during the COVID-19 pandemic, faculty development in bioethics has become increasingly important for capacity building in medical education. This study aims to determine the impact of an online course on faculty development in teaching bioethics.

Methods
This quasi-experimental study involved twenty-six multidisciplinary faculty members who participated in an online bioethics course from January 4th to 22nd, 2022. Kirkpatrick’s evaluation model was used to assess the participants’ reactions, knowledge, and behavior, using online questionnaires and direct observation by expert faculty. SPSS 25 was used for data analysis.

Results
The Kirkpatrick evaluation model showed that the course was highly satisfactory in terms of content and delivery, with a significant improvement in clinical faculty’s knowledge from pre-test to post-test (14.18 ± 1.601 to 16 ± 2.049, p < 0.05). The participants demonstrated an improved range of teaching and learning methods.

Conclusion
The online course on bioethics successfully improved the clinical faculty’s knowledge and overall approach to teaching bioethics. These findings highlight the importance of well-constructed faculty development programs, particularly during times of resource constraint such as the COVID-19 pandemic.
The value of the neutrophil to lymphocyte ratio and PLT count for the diagnosis and prediction of COVID-19 severity
Authors: Chen Yingji , Han Pingyang , Gao Yunjie , Jiang Ruifeng , Tao Mei , Li Ximin 
Published: October 2023

BackgroundCOVID-19 and influenza A can cause severe respiratory illness. Differentiating between the two diseases and identifying critically ill patients in times of epidemics become a challenge for frontline medical staff. We sought to investigate whether both diseases and their severity could be recognized by routine blood parameters.MethodsOur retrospective study analysed the clinical data and first-time routine blood parameters of 80 influenza A patients and 123 COVID-19 patients. COVID-19 patients were divided into three groups according to treatment modalities and outcomes: outpatient group, inpatient without invasive mechanical ventilation (IMV) group, and inpatient with IMV group. We used the Mann-Whitney and Kruskal-Wallis tests to analyze the differences in routine blood parameters between the two or three groups. Receiver operating characteristic (ROC) curve analysis and area under the curve (AUC) were used to assess the diagnostic accuracy.ResultsCompared with outpatient influenza A patients, outpatient COVID-19 patients had a higher neutrophil to lymphocyte ratio (NLR) (6.63 vs 3.55). ROC analysis showed that the NLR had a high diagnostic value for differentiating COVID-19 from influenza A (AUC = 0.739). The best cut-off point of the NLR was 6.48, the diagnostic sensitivity was 0.523, and the specificity was 0.925. The median platelet (PLT) count in the different COVID-19 groups was as follows: outpatient group (189×109/L), inpatient without IMV group (161×109/L), and inpatient with IMV group (94×109/L). Multivariate logistic regression analysis found a significant association between PLT and treatment modality and outcome in COVID-19 patients (p<0.001).ConclusionsNLR can be used as a potential biological indicator to distinguish COVID-19 and influenza A. Decreased PLT predicts the critical condition of COVID-19 patients and helps stratify the treatment of COVID-19 patients.
Cyasterone has a protective effect on steroid-induced Osteonecrosis of the femoral head
Authors: Sun Youqiang , Liang Mengmeng , Xing Yuemeng , Duan Yinfan , Zhang Shuangxiao , Deng Baogui , Xiang Xiaobing , Zhou Bengen 
Published: October 2023

Context
Cyasterone alleviated the apoptosis of BMSCs induced by Dexamethasone via the PI3K/AKT signaling pathway. In addition, Cyasterone had a protective effect on SIONFH model rats by reducing the percentage of empty bone lacunae.

Objective
To study the effect of Cyasterone on apoptosis of rat BMSCs and its function on the SIONFH rat model.

Methods
Rat BMSCs were cultured and divided into Control, DXM and Cyasterone (DXM+Cyasterone) groups. The apoptosis of each group was detected by flow cytometry, the expressions of Caspase-3 and Caspase-9 were detected by immunofluorescence staining, and the mRNA and protein expressions of AKT, BAX, P53, P85, Bcl-2 and Cytochrome C were detected by qPCR and WB. In animal experiments, the femoral head of rats were subjected to HE staining and Micro-CT to observe the necrosis and repair conditions.

Results
The apoptosis rate of DXM and Cyasterone groups increased compared with Control group, and the apoptosis rate of Cyasterone group decreased compared with DXM group. Compared with DXM group, the mRNA expression of BAX, P53, P85 and Cytochrome C in Cyasterone group were increased, while the protein expression of AKT and Bcl-2 decreased. The histopathological and morphological analysis showed that Cyasterone promoted the trabecular bone structure in rat, which evenly benefit for the repair of SIONFH.

Conclusion
Cyasterone can reduce the apoptosis of rat BMSCs induced by Dexamethasone, and help promoting the bone repair in SIONFH rats.
Stability analysis, phase plane analysis, and isolated soliton solution to the LGH equation in mathematical physics
Authors: Islam S. M. Rayhanul , Ahmad Hijaz , Khan Kamruzzaman , Wang Hanfeng , Akbar M. Ali , Awwad Fuad A. , Ismail Emad A. A. 
Published: October 2023

Abstract
In this article, we investigated the Landau–Ginzburg–Higgs (LGH) equation, focusing on the analysis of isolated soliton solutions and their stability. To compute the isolated soliton solutions, we used the advanced auxiliary equation (AAE) approach, which has proven to be a powerful and efficient method for extracting soliton solutions in various nonlinear partial differential equations (NLPDEs). We provided a detailed explanation, both graphically and physically, of the obtained soliton solutions in this article. Furthermore, we used the linear stability technique to conduct a stability analysis of the LGH equation. Additionally, we studied the bifurcation and stability of the equilibria and performed phase plane analysis of the model. We also provided a discussion on the comparisons between the AAE method and two other well-known approaches: the generalized Kudryashov method and the improved Bernoulli sub-equation function method. The application of the AAE approach in this study demonstrates its effectiveness and capability in analysing and extracting soliton solutions in NLPDEs.
Selection of Key Performance Indicators for Your Sport and Program: Proposing a Complementary Process-Driven Approach
Authors: Clubb Jo , Allen Sian Victoria , Yung Kate K. 
Published: October 2023

ABSTRACT
Key performance indicators (KPIs) are commonplace in business and sport. They offer an objective means to link data and processes with performance outcomes. Yet, their application in sports performance, particularly team sports, is not without issue. Here, we review 4 key issues relating to KPI application in team sports; lack of a universal definition, complexity of performance, drifting from on-field performance goals with off-field targets, and agency issues across different key stakeholders. With these issues relating to sports performance KPIs in mind, we propose a complementary approach to help practitioners focus on implementing the conditions that create performance environments and opportunities for success in a complex sporting environment. Ongoing process trackers (OPTs) are quantifiable measures of the execution of behaviors and processes that create the environments, cultures, and conditions for successful performance outcomes. This approach equips sports science practitioners with key questions they can ask themselves and their team when starting to select and use OPTs in their program.
Stimulus-Induced Theta-Band LFP Oscillations Format Neuronal Representations of Social Chemosignals in the Mouse Accessory Olfactory Bulb
Authors: Cohen Oksana , Kahan Anat , Steinberg Idan , Malinowski Sebastian T. , Rokni Dan , Spehr Marc , Ben-Shaul Yoram 
Published: October 2023

Social communication is crucial for the survival of many species. In most vertebrates, a dedicated chemosensory system, the vomeronasal system (VNS), evolved to process ethologically relevant chemosensory cues. The first central processing stage of the VNS is the accessory olfactory bulb (AOB), which sends information to downstream brain regions via AOB mitral cells (AMCs). Recent studies provided important insights about the functional properties of AMCs, but little is known about the principles that govern their coordinated activity. Here, we recorded local field potentials (LFPs) and single-unit activity in the AOB of adult male and female mice during presentation of natural stimuli. Our recordings reveal prominent LFP theta-band oscillatory episodes with a characteristic spatial pattern across the AOB. Throughout an experiment, the AOB network shows varying degrees of similarity to this pattern, in a manner that depends on the sensory stimulus. Analysis of LFP signal polarity and single-unit activity indicates that oscillatory episodes are generated locally within the AOB, likely representing a reciprocal interaction between AMCs and granule cells. Notably, spike times of many AMCs are constrained to the negative LFP oscillation phase in a manner that can drastically affect integration by downstream processing stages. Based on these observations, we propose that LFP oscillations may gate, bind, and organize outgoing signals from individual AOB neurons to downstream processing stages. Our findings suggest that, as in other neuronal systems and brain regions, population-level oscillations play a key role in organizing and enhancing transmission of socially relevant chemosensory information.SIGNIFICANCE STATEMENTThe accessory olfactory bulb (AOB) is the first central stage of the vomeronasal system, a chemosensory system dedicated to processing cues from other organisms. Information from the AOB is conveyed to other brain regions via activity of its principal neurons, AOB mitral cells (AMCs). Here, we show that socially relevant sensory stimulation of the mouse vomeronasal system leads not only to changes in AMC activity, but also to distinct theta-band (∼5 Hz) oscillatory episodes in the local field potential. Notably AMCs favor the negative phase of these oscillatory events. Our findings suggest a novel mechanism for the temporal coordination of distributed patterns of neuronal activity, which can serve to efficiently activate downstream processing stages.
HIV Preexposure Prophylaxis Provision among Adolescents: 2018 to 2021
Authors: Kimball Anne A. , Zhu Weiming , Leonard Jerome , Wei Wei , Ravichandran Ishwarya , Tanner Mary R. , Huang Ya-Lin A. , Hoover Karen W. , Kourtis Athena P. 
Published: October 2023

BACKGROUND AND OBJECTIVES
HIV preexposure prophylaxis (PrEP) is safe, effective, and was approved for adolescents in 2018. Adolescents and young adults make up 20% of HIV diagnoses in the United States. Our objective was to describe trends in adolescents prescribed PrEP during 2018 through 2021 and characteristics of these adolescents and their PrEP providers.

METHODS
We identified adolescents aged 13 to 19 years with oral PrEP prescriptions during 2018 through 2021 in a national pharmacy database using a validated algorithm. We assessed trends by calculating the overall percentage change and estimated annual percentage change with 95% confidence intervals. We described characteristics of adolescents and their PrEP providers in 2021. We performed χ2 analyses to assess differences by sex and age group.

RESULTS
The number of adolescents prescribed PrEP increased 76.2% from 2018 to 2021 (estimated annual percentage change: 18.0% [95% confidence interval: 16.6–19.5]), despite decreases in 2020. We observed increases among all sex and age groups, with larger increases among older adolescents aged 18 to 19 years. The majority of the 6444 adolescents prescribed PrEP in 2021 were male (82.6%) and aged 18 to 19 years (87.8%). Among 2455 physician PrEP providers, 29.6% were pediatricians, with varying specialty distributions by adolescent age group (P &lt; .001). Among the 217 pediatricians who prescribed PrEP to adolescents aged 13 to 17 years, 67.7% were general pediatricians.

CONCLUSIONS
PrEP provision for adolescents has increased, largely among older and male adolescents. The availability of PrEP provides an important opportunity for pediatric providers to take an active role in HIV prevention.
Stable hydrogen isotope compositions of tourmalines from the Sopron metamorphic complex: Metamorphic closure temperature and fluid composition
Authors: Demény Attila 
Published: October 2023

AbstractStable hydrogen isotope compositions of metamorphic rocks and minerals can provide information on the origin of metamorphic fluids, which is especially important in systems that had experienced multiple metamorphic events. The Sopron orthogneiss-micaschist complex is a good target as it records signs of Variscan and Alpine metamorphic events as well as Variscan granitic magmatism. In this study tourmaline-bearing rocks (pegmatitic orthogneisses and kyanite-chlorite-muscovite schists) of the Sopron metamorphic complex were sampled and their tourmaline grains were analyzed for stable hydrogen isotope compositions (δ2H). The δ2H values (−23 ± 1‰, relative to V-SMOW) are in accordance with a fluid flux from devolatilization of subducted, seawater-containing rocks. Tourmaline-chlorite hydrogen isotope fractionations correspond to about 550 °C, indicating that δ2H values formed close to peak metamorphic temperatures are preserved without retrograde isotope exchange during cooling.
Desarrollo de la plataforma Rutia para la innovación y optimización en la gestión de procesos de transporte de pasajeros
Authors: Palencia-García Deivis , Cuevas-Rojas Luis , Marriaga-Barroso Angie , Martelo-Ching Ronald , Ovallos Gazabon David 
Published: October 2023

Objetivo: Presentar el desarrollo de Rutia, un ecosistema de soluciones tecnológicas que incluyen elementos de inteligencia artificial, y analítica de datos para administrar rutas en el transporte de pasajeros en modalidad empresarial, turística y de transporte escolar además de los contratos y otras operaciones involucradas. Metodología: Se desarrolló la solución tecnológica a través de tres (3) fases Diseño y ajuste de la plataforma Rutia, Desarrollo y validación de nuevas funcionalidades y Despliegue de la estrategia comercial. Este trabajo presenta los resultados de su validación en ambiente real de la solución con un grupo de cinco (5) early adopters, lo que permitió la retroalimentación y validación de las funcionalidades de la plataforma. Resultados: La solución permite la optimización e Integración con GPS, aplicativos Móviles, digitalización de información y reportes de interés para el sector. Conclusiones: En términos generales la plataforma cumple con las expectativas de los usuarios e impacta de manera positiva en la productividad de los vehículos (aumento promedio del 21%), lo que se refleja en la disminución de tiempos de ejecución de ruta (12% en promedio), disminución de costos de ruta (12% en promedio) e incremento del volumen de la operación y satisfacción de usuarios.
Autonomous navigation and indoor mapping for a service robot
Authors: Jimenez Builes Jovani Alberto , Acosta Amaya Gustavo , López Velásquez Julián 
Published: October 2023

Abstract
Objective: Simultaneous Localization and Mapping (SLAM) is a quite common and interesting problem in mobile robotics. It is the basis of safe autonomous navigation of mobile robots and the entrance to new combined applications with a manipulator for instance. Method: In order to find a solution to the SLAM problem, the ROS middleware and the MRPT were selected. Autonomous navigation was tested using two methods, the MRPT navigation ROS package, which is a reactive navigation method based on Trajectory Parameter Space (TP-Space) transformations, and the ROS navigation stack, a standard for differential drive and holonomic wheeled robots. Results: To validate the advantages and disadvantages of both approaches, a mobile robot with strong kinematic constraints (Ackermann-steering-type) known as Summit was used. As an additional work, an application using the mobile robot Summit and a robotic manipulator (Powerball) was carried out, with the intention of picking and placing objects of the mobile robot, a widely spread application among service robotics, especially, in the area of industrial logistics. Conclusions: Finally, it is concluded that with the tests carried out with the robot, it was possible to demonstrate autonomous navigation, using the two mentioned methods.
El adolescente penalmente responsable: una perspectiva en línea con los objetivos de desarrollo sostenible
Authors: Cabrera Izquierdo Lorena Elizabeth , Mejia Turizo Jorge , Ferrer-Herrera María Angélica , Lastra Mier Roberto 
Published: October 2023

El presente artículo tiene como finalidad realizar un abordaje sobre la normativa internacional y nacional aplicable al adolescente penalmente responsable, y su estrecha relación, en cuanto al aspecto preventivo, con los objetivos de desarrollo sostenible, especialmente en metas de paz, justicia e instituciones sólidas. Para tales propósitos se acudió a una metodología con enfoque cualitativo, deductiva, aplicando un paradigma hermenéutico y teniendo como técnica de recolección de información el análisis documental. En cuanto a resultados se encontró que un desempeño eficaz en políticas de prevención de delincuencia juvenil puede consolidarse como un indicador de cumplimiento de metas de los Objetivos de Desarrollo Sostenible (lease ODS), fundamentalmente las relativas a solidez institucional, paz y justicia, aunque como conclusión se expone que hace falta incrementar cualitativa y cuantitativamente los esfuerzos institucionales de autoridades colombianas para alcanzar concreción y materialización de instrumentos nacionales e internacionales de prevención al crimen, resocialización y protección integral, ello es analizado más allá de su simple visión correctiva, sino en su enfoque de atención plena, entendiendo desde las causas estructurales, que los adolescente no son en esencia victimarios, sino víctimas de un sistema que no brinda las condiciones para un crecimiento y formación integral.
Geopolitical luck or crisis?:Communicating China’s Built and Road Initiative amidst South China Sea Dispute
Authors: Jim Duran  
Published: October 2023

As the maritime dispute in the South China Sea (West Philippine Sea) continues, this paper speculates on the possible outcomes of former Philippine President Rodrigo Duterte's sustained commitment to have stronger trade ties with China by participating in the Built and Road Initiative (BRI). During Chinese President Xi Jinping's official visit to the United States last year, 29 bilateral agreements were inked. When it comes to military, security, law enforcement, maritime cooperation, transnational crime, and other issues, China, Asia's biggest economy, has both agreements and worries. Even if mistrust in Asia's economic superpower is growing due to debt trap concerns and a maritime dispute, there is still hope for achieving the American goal of financial security because of the government's cooperative influence on China's power aspirations. Although estimates on total investments differ, Chatzky and McBride (2019) cite Morgan Stanley as saying China's entire costs throughout the duration of the Built and Road Initiative (BRI) might reach $1.2-1.3 trillion by 2027.
In conclusion, the Philippines and other Southeast Asian nations need to have room for conversation and cooperation in order to construct shared progress. According to Gou and Yang (2017), it is clear that all Asian nations want to maintain cordial ties with China. China's US$40 billion funding schemes, via the 21st century Maritime Silk Road, may accelerate regional infrastructure development and integration, and there are strong cultural and people-to-people links. The economy and increasing middle class provide significant prospects.