bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of ebony as a potential selectable marker for genetic sexing in Aedes aegypti
Authors: Nikolouli Katerina , Compton Austin , Tu Zhijian Jake , Bourtzis Kostas 
Published: February 2025

Abstract

Background

Aedes aegypti is expected to invade previously unoccupied areas, mainly due to the climate change, the increase in travel and trade activities and the continuous transformation of the rural environment into urban areas. The sterile insect technique (SIT), which relies on the mass production and release of sterile males, is an environmentally friendly approach that can be applied for population control of Ae. aegypti. SIT programs can be greatly benefited by a genetic sexing strain (GSS) and a reliable sex sorting system to minimize any accidental female release. Visually detectable or conditionally lethal selectable markers can be used for the development of new GSSs. In this study, we evaluated the suitability and competence of a mutant Ae. aegypti ebony strain for the development of a new GSS. The ebony gene is known to be involved in the pigmentation pathway of several dipteran insects, including Ae. aegypti.

Methods
An ebony gene knockout was developed though CRISPR/Cas9 mutagenesis. G0 individuals with the desired phenotype were crossed, and progeny were screened in every generation. PCR and sequencing were performed using gDNA from a pulled leg to determine the mutant genotype. Quality control tests, including pupae and adult recovery rates, male sex ratio and fecundity, were applied to the ebony mutant line to determine whether the mutation confers any fitness cost.

Results
An Ae. aegypti ebony knockout mutant carrying a 5-bp deletion was obtained, which presented darker head and siphon phenotypes at the larval stage. However, genetic analysis revealed that this ebony mutation results in incomplete penetrance and variable expressivity. The establishment of a pure ebony mutant line was not possible because of the fitness costs conferred by the mutation.

Conclusions
In this study, the adequacy and suitability of the ebony gene as a selectable marker for the development of a GSS in Ae. aegypti were assessed. Despite its clear phenotype early in larval development, the homozygous mutant line presented phenotypic inconsistency and loss of fertility. These drawbacks clearly indicate that this particular mutation is not suitable for the development of a new GSS. Nonetheless, it cannot be excluded that a different mutation will lead to a different expression and penetrance profile and a viable homozygous mutant line.

Graphical Abstract
Sex-dependent effects of a high-fat diet on the hypothalamic response in mice
Authors: Dreux Virginie , Lefebvre Candice , Breemeersch Charles-Edward , Salaün Colin , Bôle-Feysot Christine , Guérin Charlène , Déchelotte Pierre , Goichon Alexis , Coëffier Moïse , Langlois Ludovic 
Published: February 2025

Abstract
Sex differences in rodent models of diet-induced obesity are still poorly documented, particularly regarding how central mechanisms vary between sexes in response to an obesogenic diet. Here, we wanted to determine whether obese phenotype and hypothalamic response to a high-fat diet (HFD) differed between male and female C57Bl/6J mice. Mice were exposed to either a 60% HFD or a standard diet first for both a long- (14 weeks) and shorter-periods of time (3, 7, 14 and 28 days). Analysis of the expression profile of key neuronal, glial and inflammatory hypothalamic markers was performed using RT-qPCR. In addition, astrocytic and microglial morphology was examined in the arcuate nucleus. Monitoring of body weight and composition revealed that body weight and fat mass gain appeared earlier and was more pronounced in male mice. After 14 weeks of HFD exposure, normalized increase of body weight reached similar levels between male and female mice. Overall, both sexes under HFD displayed a decrease of orexigenic neuropeptides expression while an increase in Pomc gene expression was observed only in female mice. In addition, changes in the expression of hypothalamic inflammatory markers were relatively modest. We also reported that the glial cell markers expression and morphology were affected by HFD in a sex- and time dependent manner, suggesting a more pronounced glial cell activation in female mice. Taken together, these data show that male and female mice responded differently to HFD exposure, both on short- and long-term and suggest that a strong inflammatory hypothalamic profile is not systematically present in diet-induced obesity models. Nevertheless, in addition to these present data, the underlying mechanisms should be deciphered in further investigations.
Tranexamic acid in spontaneous intracerebral hemorrhage: a meta-analysis
Authors: Chang Yu , Lai Pei-Chun , Huang Chih-Yuan , Song Junmin , Chi Kuan-Yu , Hung Hsiang-Yi , Huang Yen-Ta 
Published: February 2025

Abstract

Background
Spontaneous intracerebral hemorrhage (sICH) is a critical and disabling form of stroke and accounts for an obvious number of stroke-related deaths and disabilities globally. Hematoma growth is a key target for therapeutic intervention because of its association with poor outcomes. Recently, the STOP–MSU trial showed that intravenous tranexamic acid (TA) did not reduce hematoma growth or improve clinical outcomes when administered within 2 h of intracerebral hemorrhage symptom onset. This study aims to evaluate the efficacy of TA in reducing hematoma growth and improving clinical outcomes in patients with spontaneous sICH by incorporating the findings from the latest STOP–MSU trial and consolidating past research to clarify the overall efficacy and safety of TA on sICH.

Methods
A systematic review and meta-analysis were conducted according to the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA guidelines. We included randomized controlled trials (RCTs) comparing TA to placebo in adult patients with sICH. Databases such as PubMed, Medline, and Cochrane were searched up to May 2024. Key outcomes analyzed included hematoma expansion, mortality within 90 days, thromboembolic events, and favorable functional outcomes. Data were pooled using a random-effects model and analyzed using the “metafor” package in RStudio.

Results
Five RCTs involving 1419 patients were included. The meta-analysis showed no significant difference in hematoma expansion (odds ratio [OR] 0.87, 95% confidence interval [CI] 0.74–1.03), mortality within 90 days (OR 1.03, 95% CI 0.86–1.24), thromboembolic events (OR 1.07, 95% CI 0.69–1.64), and favorable functional outcomes (modified Rankin Scale of 0–2 at 90 days; OR 1.04, 95% CI 0.88–1.22) between the TA and placebo groups.

Conclusions
TA does not significantly reduce hematoma growth or improve clinical outcomes in patients with sICH. Despite its affordability and availability, the routine use of TA in sICH is not supported by current evidence.
Immunogenicity of monkeypox virus surface proteins and cross-reactive antibody responses in vaccinated and infected individuals: implications for vaccine and therapeutic development
Authors: Liu Jing , Wang Xun , Zhang Yiting , Liu Changyi , Zhang Meng , Li Chen , Liu Peiling , Li Shanshan , Wei Kaifeng , Cai Yiming , Yu Hongjie , Hu Zhiliang , Wang Pengfei , Zhang Yanliang 
Published: February 2025

Abstract

Background
The monkeypox virus (MPXV) has raised global health concerns due to its widespread transmission. This study evaluated the MPXV immunogenic antigens and the impact of vaccinia virus (VACV) vaccination and MPXV infection on cross-reactive antibody responses to conserved proteins from representative MPXV strains that reflected the evolutionary trajectory.

Methods
Phylogenetic analyses were first conducted to reveal the evolutionary trajectory of MPXV from 1970 to 2024. A total of 84 serum samples were collected: 42 from VACV-vaccinated individuals, 12 from MPXV-infected participants in the early stage, 13 from the late stage, and 17 from naive individuals. Demographic data, MPXV and HIV status, as well as other clinical information were collected using standardized forms. Immunogenicity, cross-reactive antibody responses, and amino acid similarity to 15 MPXV surface proteins were assessed using enzyme-linked immunosorbent assays, VACV neutralization tests, and sequence alignment. Data analysis methods included analysis of variance, Mann–Whitney U test, binary logistic regression, Pearson correlation, and linear regression, with a significance threshold of P < 0.05.

Results
The 186 complete genome sequences were classified into different clades and lineages, ranging from clade Ia to clade IIb C.1.1. Individuals infected with MPXV demonstrated strong antibody responses to antigens A35R, B6R, H3L, and E8L. VACV-vaccinated individuals exhibited broader cross-reactivity, particularly against A21L (P = 0.0003), A28L (P = 0.0028), A29L (P = 0.0324), G2R (P = 0.0003), and H2R (P = 0.0008), compared to MPXV-infected individuals. Pearson correlation analysis revealed significant associations (P = 0.0049) between antibody responses and the amino acid sequence similarity with other orthopoxviruses. Furthermore, MPXV-infected individuals exhibited greater neutralizing activity against VACV than those VACV-vaccinated individuals (P < 0.0001), while the vaccinated group retained cross-protective immunity even decades post-vaccination.

Conclusions
A35R, B6R, H3L, and E8L are the main immunogenic antigens of MPXV. VACV-vaccination triggers a cross-reactive antibody response to MPXV surface proteins. Our findings suggest the need for targeted vaccines and antibody treatments for MPXV, as well as the reintroduction of smallpox vaccinations with booster doses for high-risk groups.

Graphical Abstract
Evaluation of lateral flow devices for rabies diagnosis in decomposed animal brain samples
Authors: Todoroki Ryota , Ongtangco Joely T. , Kimitsuki Kazunori , Saito Nobuo , Mananggit Milagros R. , Velasco Cornelio R. , Mauhay Jaira D. , Garcia Alyssa M. , Demetria Catalino S. , Kentaro Yamada , Nishizono Akira 
Published: February 2025

Abstract

Background
The Direct Fluorescent Antibody Test (DFAT), the standard rabies confirmatory test, is less sensitive when used with decomposed brain samples, a frequent issue in rabies-endemic regions. This study evaluates the diagnostic accuracy of the ADTEC lateral flow device (LFD) for rabies post-mortem diagnosis using decomposed brain samples.

Methods
We used 34 animal heads submitted with a cold chain to an animal diagnostic laboratory located in central Philippines including 26 DFAT-positive and eight DFAT-negative samples. After defrosting the heads, the entire brain was extracted and left at room temperature to induce decomposition. The decomposition status was scored after 1 day, 3 days, and 4 days at room temperature. DFAT and LFD were performed using the brain samples at each timepoint to evaluate the diagnostic accuracy. The day of animal head submission to the laboratory was defined as day 0, and the DFAT results were used as the reference. Image analysis was performed to measure the intensity of the LFD-positive bands.

Results
The decomposition scores dropped by day 3 and day 4, with all samples exhibiting signs of advanced decomposition. The sensitivity of DFAT was 96.2% (95% confidence interval 80.4–99.9) on day 1, but dropped to 61.5% (P < 0.01) by day 3 and further decreased to 38.5% (20.2–59.4) by day 4. In contrast, the sensitivities and specificities of LFD on day 1, day 3, and day 4 were consistently 100% (86.3–100) and 100% (63.1–100), respectively. Visual examination of the test band intensity on the LFD suggested that the intensity of the positive bands increased as decomposition progressed.

Conclusions
ADTEC LFDs demonstrated consistently high sensitivity and specificity with decomposed brain samples observed up to day 4, making them a reliable screening tool for rabies post-mortem diagnosis in decomposed brain samples, particularly in resource-limited settings. Furthermore, LFD positive bands became more distinct as decomposition advanced.
Research on building energy consumption prediction algorithm based on customized deep learning model
Authors: Liang Zheng , Chen Junjie 
Published: February 2025

Abstract
Forecasting energy usage in buildings is essential for implementing energy saving measures. Precisely forecasting building energy use is difficult due to uncertainty and noise disruption.To achieve enhanced accuracy in predicting energy use in buildings, a deep learning approach is proposed. This paper proposes a customized convolutional neural network with Q-Learning (CCNN-QL) based reinforcement learning algorithm for predicting energy consumption in building.The suggested CCNN-QL model offers an auto-learning feature that predicts building energy consumption through an automated method, continually improving its predictive accuracy.To assess its performance, various building types were selected to study the factors influencing excessive energy consumption, and data were collected from multiple Chinese cities. The suggested model’s performance has been assessed using evaluation metrics, resulting in a low Mean Absolute Error (MAE) and Root Mean Square Error (RMSE), indicating superior accuracy relative to comparable studies. Experimental results indicate that the suggested technique has superior predictive performance across several scenarios of building energy usage.
Scientific knowledge and wisdom of kimchi: a blessing Korean
Authors: Kwon Dae Young , Chung Kyung Rhan , Lee Chang Hyeon , Kim Soon-Hee , Daily James W. , Park Sunmin 
Published: February 2025

Abstract
Kimchi, a unique traditional fermented food of Korea, is a product of agricultural necessity and scientific serendipity. This blessed food, born from the efforts of Korean women striving to create delicious and lasting sustenance, has become an integral part of Korean cuisine. Always present in the traditional Korean bapsang, which means meal table setting alongside bap (rice), kuk (soup), and banchan (side dishes), kimchi's appeal remains constant despite frequent consumption. The origin and evolution of kimchi are intrinsically linked to Korea's history, with fermentation techniques and ingredients adapting to diverse regional and social environments over time. While contemporary research often focuses on kimchi processing and its health benefits, fewer studies explore the fermentation mechanisms, historical background, safety aspects, and preservation techniques of kimchi. Understanding the nature of kimchi requires a comprehensive examination of its biological role and historical context. This manuscript reviews kimchi fermentation from scientific and cultural perspectives, covering ingredients, microorganisms, fermentation mechanisms, and preservation history. We also discuss the safety, palatability, and health benefits of kimchi. The development of kimchi reflects centuries of culinary innovation by Korean women, who created yangnyom (seasonings) and fermented foods to enhance the nutritional value and flavor of bap, ultimately shaping the unique and delightful Korean cuisine we know today.
Influence of Termite Hill Soil as a partial cement replacement in mortar production
Authors: Tebabal Melkie Gedamu , Yifru Begashaw Worku , Getachew Ephrem Melaku , Yehualaw Mitiku Damtie 
Published: February 2025

Abstract
This study investigates the potential of Termite Hill Soil (THS) as a sustainable partial replacement for Ordinary Portland Cement (OPC) in mortar production. The experimental program involved preparing mortar mixtures with 0%, 5%, 10%, 15%, and 20% THS replacement levels by weight while maintaining a constant water-to-cement ratio. Various physical, chemical, fresh, mechanical, durability and microstructural properties were studied. The findings indicate that THS possesses pozzolanic properties. Workability and setting time decreased, while the water demand for normal consistency increased as THS content rose. The compressive strength results showed a 9.5% improvement at 5% THS and achieved 98% of the control mix at 10% THS. Additionally, THS5 and THS10 significantly enhanced bulk density, ultrasonic pulse velocity, and compressive strength, particularly in the latter ages of mortar. The microstructural analysis confirmed improved density in these mixtures, along with respectable thermal properties and FTIR spectra. Overall, THS showcases promising and commendable performance, and its utilization as a substitute for OPC not only reduces the carbon footprint of cement production but also promotes sustainability within the construction industry.
Incorporating Complementary Therapies Into Diabetes Care
Authors: Blanchette Julia E , Paquin Forrest , Dobbs Brandi N , Kiely Rebecca L , Hatipoglu Betul 
Published: February 2025

Abstract

Context
Current diabetes care and education programs and expert clinical diabetes management guidelines focus on diabetes self-care behaviors and have yet to incorporate complementary therapies. Complementary therapies, such as music therapy, yoga, mindfulness, and art therapy, have been used globally for centuries and have positive metabolic and glycemic outcomes. In this mini-review, we describe complementary therapies successfully used in diabetes, identify current evidence-based practice gaps, and provide recommendations for incorporating complementary therapies into diabetes care.

Evidence Acquisition
We thoroughly searched relevant PubMed and Google Scholar studies from 2004 to 2024. Our inclusion criteria were clinical trial studies using the search terms “diabetes self-management” OR “metabolic outcomes” OR “diabetes” OR “type of complementary therapy (music therapy, mindfulness, yoga or art therapy) OR population (type 1 diabetes, type 2 diabetes, prediabetes, diabetes).”

Evidence Synthesis
We synthesized the evidence to determine complementary therapies (music therapy, mindfulness, yoga, or art therapy) that benefit individuals with diabetes. Findings showed that complementary therapies support diabetes-related psychological and cardiometabolic outcomes and enhance the Association of Diabetes Care and Education Specialists 7 Self-Care Behaviors for diabetes self-management, specifically healthy coping, monitoring, reducing risks, and problem-solving. Critical gaps included the lack of large-scale randomized controlled trials in North American diabetes self-management education programs.

Conclusion
Complementary therapies have positive psychological and physiological health benefits for people living with diabetes, yet more randomized controlled trials are needed to assess their effectiveness on a large scale. In the interim, complementary therapies can be integrated into diabetes education, specifically as adjunctive hands-on therapies to enhance self-management behaviors and meet self-management goals.
Navigating the Spectrum of 4 Evidence-Based Nutrition Options for Type 2 Diabetes Management
Authors: Baskin Revital Gorodeski , Karp Kimberly A 
Published: February 2025

Abstract

Context
Type 2 diabetes mellitus is a complex, pervasive disease that creates a health and financial burden. The goal is to head off progression to insulin requirement, and dietary changes have been recognized as a first-line intervention in the treatment protocol. Four evidence-based nutritional options are reviewed and their benefits for metabolic consequences.

Evidence Acquisition
We conducted a comprehensive search of relevant literature using the PubMed database for recent systematic reviews, meta-analyses, and original research articles.

Evidence Synthesis
The Mediterranean and plant-based diets have demonstrated benefits for the prevention and treatment of diabetes, weight reduction, lipid improvements, and overall cardiovascular risk reduction. A low carbohydrate diet is another viable option for hemoglobin A1c (HbA1c) reduction, though it is not as uniformly defined and is difficult to maintain long term. The ketogenic diet is similarly very restrictive and produces significant HbA1c reductions.

Conclusion
Type 2 diabetes prevention and treatment is crucial in this widespread disease. The first-line treatment is lifestyle modifications with dietary interventions being at the forefront. With a unique clinical model and a variety of dietary options, our program strives for individualized evidence-based care. Nutritional modifications are the best initial approaches to overcoming insulin resistance and hyperinsulinemia. All dietary modifications, like any medical intervention, require education on benefits vs risks.
Insights on Improving Health Insurance Literacy in Diabetes
Authors: Blanchette Julia E , Nally Laura M , Wentzell Katherine , Hughes Allyson S , Allen Nancy A , Litchman Michelle L 
Published: February 2025

Abstract

Context
Many adults with diabetes cannot afford medications or health care and have low health insurance literacy, resulting in psychological distress and barriers to optimal self-management.

Objective
This mini-review aimed to explore the effectiveness of health-insurance literacy interventions and provide recommendations for diabetes practice.

Methods
We conducted a thorough search of relevant PubMed and Scopus studies from 2014 to 2024. Our inclusion criteria were interventions or pilot intervention studies using the search terms “health insurance” OR “health insurance literacy” OR “population (oncology, young adults/college/university students, and adults).” We then meticulously analyzed the quality of these studies, and assessments were conducted in Excel. We synthesized the evidence on interventions used to support health-insurance literacy across chronic health conditions and the general population, and their applications to diabetes care. Our findings showed effective interventions address a population's specific learning needs and literacy level. Multiple/diverse methods were used, including web-based decision aids, virtual sessions, hands-on support from navigators, Serious Games, educational workshops, and online microvideo Toolkits. Critical gaps included defining and measuring health-care literacy.

Conclusion
Solutions for health-insurance education include strategies to incorporate education into academic curricula, diabetes transition programs, community health fairs, and employing community health workers within health-care systems. Although there is a validated health insurance literacy measure, consistent use of this measure across future programs can further the critically needed science in this field.