bluuebunny/crossref_metadata_2025_split huggingface dataset:
Roll-to-plate 0.1-second shear-rolling process at elevated temperature for highly aligned nanopatterns
Authors: Cho Junghyun , Oh Jinwoo , Bang Joona , Koh Jai Hyun , Jeong Hoon Yeub , Chung Seungjun , Son Jeong Gon 
Published: December 2023

AbstractThe shear-rolling process is a promising directed self-assembly method that can produce high-quality sub−10 nm block copolymer line-space patterns cost-effectively and straightforwardly over a large area. This study presents a high temperature (280 °C) and rapid (~0.1 s) shear-rolling process that can achieve a high degree of orientation in a single process while effectively preventing film delamination, that can be applied to large-area continuous processes. By minimizing adhesion, normal forces, and ultimate shear strain of the polydimethylsiloxane pad, shearing was successfully performed without peeling up to 280 °C at which the chain mobility significantly increases. This method can be utilized for various high-χ block copolymers and surface neutralization processes. It enables the creation of block copolymer patterns with a half-pitch as small as 8 nm in a unidirectional way. Moreover, the 0.1-second rapid shear-rolling was successfully performed on long, 3-inch width polyimide flexible films to validate its potential for the roll-to-roll process.
Hydrophobically gated memristive nanopores for neuromorphic applications
Authors: Paulo Gonçalo , Sun Ke , Di Muccio Giovanni , Gubbiotti Alberto , Morozzo della Rocca Blasco , Geng Jia , Maglia Giovanni , Chinappi Mauro , Giacomello Alberto 
Published: December 2023

AbstractSignal transmission in the brain relies on voltage-gated ion channels, which exhibit the electrical behaviour of memristors, resistors with memory. State-of-the-art technologies currently employ semiconductor-based neuromorphic approaches, which have already demonstrated their efficacy in machine learning systems. However, these approaches still cannot match performance achieved by biological neurons in terms of energy efficiency and size. In this study, we utilise molecular dynamics simulations, continuum models, and electrophysiological experiments to propose and realise a bioinspired hydrophobically gated memristive nanopore. Our findings indicate that hydrophobic gating enables memory through an electrowetting mechanism, and we establish simple design rules accordingly. Through the engineering of a biological nanopore, we successfully replicate the characteristic hysteresis cycles of a memristor and construct a synaptic device capable of learning and forgetting. This advancement offers a promising pathway for the realization of nanoscale, cost- and energy-effective, and adaptable bioinspired memristors.
Bias-free driven ion assisted photoelectrochemical system for sustainable wastewater treatment
Authors: Dang Qi , Zhang Wei , Liu Jiqing , Wang Liting , Wu Deli , Wang Dejin , Lei Zhendong , Tang Liang 
Published: December 2023

AbstractPhotoelectrochemical (PEC) systems have emerged as a prominent renewable energy-based technology for wastewater treatment, offering sustainable advantages such as eliminating dependence on fossil fuels or grid electricity compared to traditional electrochemical treatment methods. However, previous PEC systems often overlook the potential of ions present in wastewater as an alternative to externally applied bias voltage for enhancing carrier separation efficiency. Here we report a bias-free driven ion assisted photoelectrochemical (IAPEC) system by integration of an electron-ion acceptor cathode, which leverages its fast ion-electron coupling capability to significantly enhance the separation of electrons and holes at the photoanode. We demonstrate that Prussian blue analogues (PBAs) can serve as robust and reversible electron-ion acceptors that provide reaction sites for photoelectron coupling cations, thus driving the hole oxidation to produce strong oxidant free radicals at photoanode. Our IAPEC system exhibits superior degradation performance in wastewater containing chloride medium. This indicates that, in addition to the cations (e.g., Na+) accelerating the electron transfer rate, the presence of Cl– ions further enhance efficient and sustainable wastewater treatment. This work highlights the potential of utilizing abundant sodium chloride in seawater as a cost-effective additive for wastewater treatment, offering crucial insights into the use of local materials for effective, low-carbon, and sustainable treatment processes.
Asynchronous domain dynamics and equilibration in layered oxide battery cathode
Authors: Xue Zhichen , Sharma Nikhil , Wu Feixiang , Pianetta Piero , Lin Feng , Li Luxi , Zhao Kejie , Liu Yijin 
Published: December 2023

AbstractTo improve lithium-ion battery technology, it is essential to probe and comprehend the microscopic dynamic processes that occur in a real-world composite electrode under operating conditions. The primary and secondary particles are the structural building blocks of battery cathode electrodes. Their dynamic inconsistency has profound but not well-understood impacts. In this research, we combine operando coherent multi-crystal diffraction and optical microscopy to examine the chemical dynamics in local domains of layered oxide cathode. Our results not only pinpoint the asynchronicity of the lithium (de)intercalation at the sub-particle level, but also reveal sophisticated diffusion kinetics and reaction patterns, involving various localized processes, e.g., chemical onset, reaction front propagation, domains equilibration, particle deformation and motion. These observations shed new lights onto the activation and degradation mechanisms of state-of-the-art battery cathode materials.
Transient fertilization of a post-Sturtian Snowball ocean margin with dissolved phosphate by clay minerals
Authors: Fru Ernest Chi , Bahri Jalila Al , Brosson Christophe , Bankole Olabode , Aubineau Jérémie , El Albani Abderrazzak , Nederbragt Alexandra , Oldroyd Anthony , Skelton Alasdair , Lowhagen Linda , Webster David , Fantong Wilson Y. , Mills Benjamin J. W. , Alcott Lewis J. , Konhauser Kurt O. , Lyons Timothy W. 
Published: December 2023

AbstractMarine sedimentary rocks deposited across the Neoproterozoic Cryogenian Snowball interval, ~720-635 million years ago, suggest that post-Snowball fertilization of shallow continental margin seawater with phosphorus accelerated marine primary productivity, ocean-atmosphere oxygenation, and ultimately the rise of animals. However, the mechanisms that sourced and delivered bioavailable phosphate from land to the ocean are not fully understood. Here we demonstrate a causal relationship between clay mineral production by the melting Sturtian Snowball ice sheets and a short-lived increase in seawater phosphate bioavailability by at least 20-fold and oxygenation of an immediate post-Sturtian Snowball ocean margin. Bulk primary sediment inputs and inferred dissolved seawater phosphate dynamics point to a relatively low marine phosphate inventory that limited marine primary productivity and seawater oxygenation before the Sturtian glaciation, and again in the later stages of the succeeding interglacial greenhouse interval.
Clinical evaluation of a web-based personalized recommendation system with electronic health record interface to optimize healthcare resources during SARS-CoV-2 surges
Authors: Thieme Alexander Henry , Gertler Maximilian , Piening Brar Christian , Maechler Friederike , Benzler Justus , Hartmann Claudia , Heumann Peter , Seybold Joachim , Kirchberger Valerie , Budach Volker , Mockenhaupt Frank , Mittermaier Mirja 
Published: December 2023

AbstractDuring the SARS-CoV-2 pandemic, the German healthcare system faced challenges of efficiently allocating testing resources. To address this, we developed an open-source personalized recommendation system (PRS) called “CovApp”. The PRS utilized a questionnaire to estimate the risk of infection, provided personalized recommendations such as testing, self-isolation, or quarantine, and featured QR code data transmission to electronic health records. The PRS served up to 2.5 million monthly users and received 67,000 backlinks from 1800 domains. We clinically evaluated the PRS at the SARS-CoV-2 testing facility at Charité and observed a 21.7% increase in patient throughput per hour and a 22.5% increase in patients per day. Patients using the PRS were twice as likely to belong to the High Risk group eligible for testing (18.6% vs. 8.9%, p < 0.0001), indicating successful compliance with CovApp’s recommendations. CovApp served as a digital bridge between the population and medical staff and significantly improved testing efficiency. As an open-source platform, CovApp can be readily customized to address emerging public health crises. Further, given the EHR interface, the app is of great utility for other applications in clinical settings.
Genetically predicted 486 blood metabolites concerning risk of systemic lupus erythematosus: a Mendelian randomization study
Authors: Zhao Li , Wu Ruonan , Wu Zewen , Liu Xinling , Li Jingxuan , Zhang Liyun , Zhang Shuqiu 
Published: December 2023

AbstractMetabolic abnormalities constitute a significant characteristic of systemic lupus erythematosus (SLE). We utilised a two-sample Mendelian randomisation (MR) study to evaluate the potential causal association between 486 blood metabolites and SLE. Exposure data at the metabolite level were extracted from 7824 European Genome-wide association studies (GWAS). Preliminary analysis utilised SLE GWAS data from FinnGen. The primary method for causal analysis relied on random inverse variance weighting (IVW). To ensure robustness, sensitivity analyses included the Cochran Q test, MR-Egger intercept test, MR-PRESSO, and leave-one-out analysis. Steiger testing and linkage disequilibrium score regression were employed to validate the identified metabolites. This study identified 12 metabolites, comprising six known chemical structures: 1,5-anhydroglucitol(1,5-AG) [odds ratio (OR) = 0.100, 95% confidence interval (CI): 0.015–0.773, P = 0.027), gamma-glutamylthreonine (OR = 0.077, 95% CI: 0.010–0.574, P = 0.012), 5-dodecenoate(12:1n7) (OR = 0.205, 95% CI: 0.061–0.685, P = 0.010), linoleoylglycerophosphoethanolamine * (OR = 0.159, 95% CI: 0.027–0.933, P = 0.044), erythrose (OR = 88.331,95% CI:1.098–63.214, P = 0.040) and 1-, adrenate (22:4n6) (OR = 9.876, 95% CI: 1.753–55.639, P = 0.001)]. Additionally, we found associations between SLE and six unknown chemical structures: X-06351 (OR = 0.071, 95% CI: 0.006–0.817, P = 0.034), X-10810 (OR = 4.268 95% CI: 1.260–14.459, P = 0.020), X-11412 (OR = 5.418 95% CI: 1.068–27.487, P = 0.041), X-11905 (OR = 0.551, 95%CI: 0.304–0.997, P = 0.049), X-12038 (OR = 0.178 95%CI: 0.032–0.988, P = 0.045), X-12217 (OR = 0.174 95%CI: 0.044–0.680, P = 0.014). This study offers evidence supporting a causal relationship between SLE and 12 circulating metabolites, six of which have known chemical structures and six that remain unidentified. These findings introduce a new perspective for further exploration of SLE mechanisms.
pH/redox responsive size‐switchable intelligent nanovehicle for tumor microenvironment targeted DOX release
Authors: Badparvar Fahimeh , Marjani Ahmad Poursattar , Salehi Roya , Ramezani Fatemeh 
Published: December 2023

AbstractTumor microenvironment (TME) targeted strategy could control the drug release in tumor cells more accurately and creates a new opportunity for enhanced site-specific targeted delivery. In this study, (PAA-b-PCL-S-S-PCL-b-PAA) copolymeric nanoparticles (NPs) with size-switchable ability and dual pH/redox-triggered drug release behavior were designed to significantly promote cancer uptake (cell internalization of around 100% at 30 min) and site-specific targeted doxorubicin (DOX) delivery in MDA-MB-231 tumor cells. NPs surface charge was shifted from − 17.8 to − 2.4 and their size shrunk from 170.3 to 93 nm in TME. The cell cycle results showed that DOX-loaded NPs showed G2/M (68%) arrest, while free DOX showed sub-G1 arrest (22%). Apoptosis tests confirmed that the cells treated with DOX-loaded NPs showed a higher amount of apoptosis (71.6%) than the free DOX (49.8%). Western blot and RT-PCR assays revealed that the apoptotic genes and protein levels were significantly upregulated using the DOX-loaded NPs vs. the free DOX (Pvalue < 0.001). In conclusion, dual pH/redox-responsive and size-switchable DOX-loaded NPs developed here showed outstanding anti-tumoral features compared with free DOX that might present a prospective platform for tumor site-specific accumulation and drug release that suggest further in vivo research.
Bacillus G7 improves adaptation to salt stress in Olea europaea L. plantlets, enhancing water use efficiency and preventing oxidative stress
Authors: Galicia-Campos Estrella , García-Villaraco Ana , Montero-Palmero Ma. Belén , Gutiérrez-Mañero F. Javier , Ramos-Solano Beatriz 
Published: December 2023

AbstractIn addition to genetic adaptative mechanisms, plants retrieve additional help from the surrounding microbiome, especially beneficial bacterial strains (PGPB) that contribute to plant fitness by modulating plant physiology to fine-tune adaptation to environmental changes. The aim of this study was to determine the mechanisms by which the PGPB Bacillus G7 stimulates the adaptive mechanisms of Olea europaea plantlets to high-salinity conditions, exploring changes at the physiological, metabolic and gene expression levels. On the one hand, G7 prevented photosynthetic imbalance under saline stress, increasing the maximum photosynthetic efficiency of photosystem II (Fv/Fm) and energy dissipation (NPQ) and protecting against photooxidative stress. On the other hand, despite the decrease in effective PSII quantum yield (ΦPSII), net carbon fixation was significantly improved, resulting in significant increases in osmolytes and antioxidants, suggesting an improvement in the use of absorbed energy. Water use efficiency (WUE) was significantly improved. Strong genetic reprogramming was evidenced by the transcriptome that revealed involvement of the ABA-mediated pathway based on upregulation of ABA synthesis- and ABA-sensing-related genes together with a strong downregulation of the PLC2 phosphatase family, repressors of ABA-response elements and upregulation of ion homeostasis-related genes. The ion homeostasis response was activated faster in G7-treated plants, as suggested by qPCR data. All these results reveal the multitargeted improvement of plant metabolism under salt stress by Bacillus G7, which allows growth under water limitation conditions, an excellent trait to develop biofertilizers for agriculture under harsh conditions supporting the use of biofertilizers among the new farming practices to meet the increasing demand for food.
Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes
Authors: Mysore Keshava , Njoroge Teresia M. , Stewart Akilah T. M. , Winter Nikhella , Hamid-Adiamoh Majidah , Sun Longhua , Feng Rachel Shui , James Lester D. , Mohammed Azad , Severson David W. , Duman-Scheel Molly 
Published: December 2023

AbstractG protein-coupled receptors (GPCRs), which regulate numerous intracellular signaling cascades that mediate many essential physiological processes, are attractive yet underexploited insecticide targets. RNA interference (RNAi) technology could facilitate the custom design of environmentally safe pesticides that target GPCRs in select target pests yet are not toxic to non-target species. This study investigates the hypothesis that an RNAi yeast insecticide designed to silence mosquito serotonin receptor 1 (5-HTR1) genes can kill mosquitoes without harming non-target arthropods. 5-HTR.426, a Saccharomyces cerevisiae strain that expresses an shRNA targeting a site specifically conserved in mosquito 5-HTR1 genes, was generated. The yeast can be heat-inactivated and delivered to mosquito larvae as ready-to-use tablets or to adult mosquitoes using attractive targeted sugar baits (ATSBs). The results of laboratory and outdoor semi-field trials demonstrated that consumption of 5-HTR.426 yeast results in highly significant mortality rates in Aedes, Anopheles, and Culex mosquito larvae and adults. Yeast consumption resulted in significant 5-HTR1 silencing and severe neural defects in the mosquito brain but was not found to be toxic to non-target arthropods. These results indicate that RNAi insecticide technology can facilitate selective targeting of GPCRs in intended pests without impacting GPCR activity in non-targeted organisms. In future studies, scaled production of yeast expressing the 5-HTR.426 RNAi insecticide could facilitate field trials to further evaluate this promising new mosquito control intervention.
Multi-objective design and optimization of squeezed branch pile based on orthogonal test
Authors: Wang Ziqi , Zhao Cunbao , Zhang Wenyue 
Published: December 2023

AbstractIn recent years, the emergence of the squeezed branch pile has presented a new avenue for civil engineering, offering a distinctive structure and favorable mechanical characteristics. Squeezed branch piles have strong compressive, uplift, and horizontal load resistance capabilities. Due to the existence of discs, the geometric parameters of squeezed branch piles are abundant but important. This article selects number of discs, disc diameter, disc squeeze angle, and disc spacing as the main influencing factors on the bearing capacity of squeezed branch piles and conducts a qualitative analysis of their mechanical properties. The aim of this article is to analyze the different bearing performances of squeezed branch piles through orthogonal experimental design, simulate test conditions using finite element software ABAQUS, obtain relevant data, and finally determine the weight ranking and optimal combination of influencing factors through range analysis to provide better guidance for engineering practices. Through multi-objective optimization design, six optimization objectives including compressive performance, compressive economic efficiency, uplift performance, uplift economic efficiency, maximum horizontal displacement and maximum bending moment of pile body were analyzed. The analysis methods used included comprehensive balance method, queue scoring method, principal component analysis method, entropy weight method, and analytic hierarchy process. The conclusions obtained are similar, and based on the judgment, the squeezed branch pile with 4 discs, disc diameter of 2.5D, disc squeeze angle of 35°, and disc spacing of 3D is considered as the optimal combination under consideration of all optimization objectives.
A nomogram model for predicting 5-year risk of prediabetes in Chinese adults
Authors: Hu Yanhua , Han Yong , Liu Yufei , Cui Yanan , Ni Zhiping , Wei Ling , Cao Changchun , Hu Haofei , He Yongcheng 
Published: December 2023

AbstractEarly identification is crucial to effectively intervene in individuals at high risk of developing pre-diabetes. This study aimed to create a personalized nomogram to determine the 5-year risk of pre-diabetes among Chinese adults. This retrospective cohort study included 184,188 participants without prediabetes at baseline. Training cohorts (92,177) and validation cohorts (92,011) were randomly assigned (92,011). We compared five prediction models on the training cohorts: full cox proportional hazards model, stepwise cox proportional hazards model, multivariable fractional polynomials (MFP), machine learning, and least absolute shrinkage and selection operator (LASSO) models. At the same time, we validated the above five models on the validation set. And we chose the LASSO model as the final risk prediction model for prediabetes. We presented the model with a nomogram. The model's performance was evaluated in terms of its discriminative ability, clinical utility, and calibration using the area under the receiver operating characteristic (ROC) curve, decision curve analysis, and calibration analysis on the training cohorts. Simultaneously, we also evaluated the above nomogram on the validation set. The 5-year incidence of prediabetes was 10.70% and 10.69% in the training and validation cohort, respectively. We developed a simple nomogram that predicted the risk of prediabetes by using the parameters of age, body mass index (BMI), fasting plasma glucose (FBG), triglycerides (TG), systolic blood pressure (SBP), and serum creatinine (Scr). The nomogram's area under the receiver operating characteristic curve (AUC) was 0.7341 (95% CI 0.7290–0.7392) for the training cohort and 0.7336 (95% CI 0.7285–0.7387) for the validation cohort, indicating good discriminative ability. The calibration curve showed a perfect fit between the predicted prediabetes risk and the observed prediabetes risk. An analysis of the decision curve presented the clinical application of the nomogram, with alternative threshold probability spectrums being presented as well. A personalized prediabetes prediction nomogram was developed and validated among Chinese adults, identifying high-risk individuals. Doctors and others can easily and efficiently use our prediabetes prediction model when assessing prediabetes risk.
Acid phosphatase-like proteins, a biogenic amine and leukotriene-binding salivary protein family from the flea Xenopsylla cheopis
Authors: Lu Stephen , Andersen John F. , Bosio Christopher F. , Hinnebusch B. Joseph , Ribeiro José M. 
Published: December 2023

AbstractThe salivary glands of hematophagous arthropods contain pharmacologically active molecules that interfere with host hemostasis and immune responses, favoring blood acquisition and pathogen transmission. Exploration of the salivary gland composition of the rat flea, Xenopsylla cheopis, revealed several abundant acid phosphatase-like proteins whose sequences lacked one or two of their presumed catalytic residues. In this study, we undertook a comprehensive characterization of the tree most abundant X. cheopis salivary acid phosphatase-like proteins. Our findings indicate that the three recombinant proteins lacked the anticipated catalytic activity and instead, displayed the ability to bind different biogenic amines and leukotrienes with high affinity. Moreover, X-ray crystallography data from the XcAP-1 complexed with serotonin revealed insights into their binding mechanisms.
Spatiotemporal high-resolution mapping of biological production in the Southern Ocean
Authors: Pan Xianliang L. , Lai Xiangxing , Makabe Ryosuke , Hirano Daisuke , Watanabe Yutaka W. 
Published: December 2023

AbstractThe Southern Ocean is considered to play an important role in the global biogeochemical cycles of carbon and nutrients. It is still unclear how biological production there impacts climate change owing to the remarkable gap between the current and the ideal state of research. Here, we proposed a new concept of combining Neural Network based parameterization of dissolved inorganic carbon with Biogeochemical Argo floats, showing simultaneously spatiotemporal distributions of net community production and resupply of carbon from the deep ocean based on the dissolved inorganic carbon change. It indicated a total net community production of 4.1 ± 0.3 Pg-C year−1 over the Southern Ocean, which accounts for 45% of global annual oceanic carbon export and is 20% higher than the previous estimates. Furthermore, net community production in the Southern Ocean has decreased by 0.8% year−1 since the 2010s, indicating that it may be contributing as positive feedback to global warming.