Polyether Antibiotics
Authors: Brimble Margaret A. 
Published: July 2004

AbstractSeveral polyether antibiotics have found commercial application as anticoccidials in poultry farming and in improvement of feed efficiency for ruminants. These antibiotics are characterized by multiple tetrahydrofuran and tetrahydropyran rings connected by aliphatic bridges, direct CC linkages, or spiro linkages. Other features include a free carboxyl function, many lower alkyl groups, and a variety of functional oxygen groups. These structural features enable transport of cations across lipid membranes. Some polyethers have found application as components in ion‐selective electrodes for use in clinical medicine or in laboratory studies involving transport studies or measurement of transmembrane electrical potential. The polyether class can be subdivided based on the number of carbon atoms in the backbone, ie, the longest chain of contiguous carbons between the carboxyl group and the terminal carbon. The 30 C skeleton group accounts for ∼60% of the polyethers for which structures have been determined. Monensin, widely used as an anticoccidial and feed efficiency enhancer, is an example of the 26 C backbone class. Maduramicin, another commercially important polyether, is shown as an example of the 30 C group with a sugar moiety. Many of the production procedures for polyethers utilize the separation of the mycelium followed by extraction using solvents such as methanol or acetone. A number of the polyethers can be readily crystallized. Polyethers such as monensin, lasalocid, salinomycin, and narasin are sold in many countries in crystalline or highly purified forms for incorporation into feeds or sustained‐release bolus devices. There are also mycelial or biomass products, especially in the United States, generally prepared by separation of the mycelium and then drying by azeotropic evaporation, fluid‐bed driers, or other types of commercial driers. Sophisticated methods have been developed for the chemical analysis of polyether antibiotics in feeds and residues that include the use of liquid chromatography–mass spectrometry (LCMS), bioautography, and enzyme‐linked immunosorbent assay (ELISA). The polyether antibiotics exhibit a broad range of biological, antibacterial, antifungal, antiviral, anticoccidial, antiparasitic, and insecticidal activities. They improve feed efficiency and growth performance in ruminant and monogastric animals. Only the anticoccidial activity in poultry and cattle, and the effect on feed efficiency in ruminants such as cattle and sheep, are of commercial interest. It is estimated that the polyether ionophores constitute > 80% of the total worldwide usage of anticoccidials. Bacteria belonging to the order Actinomycetales are the organisms reported to produce all of the polyethers. Most are secondary metabolites ofStreptomycessp. with the specieshygroscopicusandalbusaccounting for about one‐third of the antibiotics. An alternative to the Cane‐Clemer‐Westley unified stereochemical model for the biosynthesis of polyether antibiotics via cyclization of polyepoxides has been proposed. The alternative model invokes construction of polyethers via hydroxyl‐directedsynoxidative polycyclization of a hydroxypolyene. Biosynthesis of polyether antibiotics is catalyzed by a large family of polyketide synthases (PKSs) that function in a similar manner to fatty acid synthase using malonyl‐CoA, methylmalonyl‐CoA, and ethylmalonyl‐CoA as extender units for building the polyketide backbone. Many gene clusters encoding the enzymes of polyketide biosynthesis have been cloned and characterized. Combinatorial biosynthesis provides a promising new approach of growing importance for the synthesis of hybrid “unnatural” antibotics by means of genetic engineering of recombinant strains bearing the biosynthetic genes of bacterial polyketide synthases. The polyether antibiotics provide a complex molecular framework for the development of new synthetic strategies for their total synthesis. Over 16 of the polyether antibiotics have succumbed to total synthesis. Monensin and salinomycin represent ∼ 65–70% of worldwide usage of polyether antibiotics for controlling coccidiosis. Lasalocid, narasin, and maduramicin make up the remainder. Other compounds for coccidiosis control include nicarbazine, halofunginone, amprolium, and robenidine.