bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disjunct habitat of cryptic Terebellides (Annelida, Trichobranchidae) species shows a phylogenetic link between polychaetes from the White and the North Seas
Authors: Kudryavtseva A. A. , Novoyatlova U. S. , Chuyko A. , Gaeva D. R. , Vlasov A. V. , Manukhov I. V. 
Published: December 2023

AbstractUnderstanding the distribution and biodiversity of marine species is crucial for developing effective conservation strategies and maintaining the health of global ecosystems. Advancements in molecular data utilization have significantly improved our understanding of biodiversity within the genus Terebellides. In this study, we conducted a phylogenetic analysis on polychaete samples from the Kandalaksha Bay, White Sea, revealing their affiliation with a putative undescribed species of the genus Terebellides found in two locations of the North Sea. Interestingly, this species was not detected in the Norwegian and Barents Seas, leading us to propose a disjunct distribution scenario for this Terebellides species. This unique distribution pattern might be attributed to the succession of polychaetes by new species, facilitated by the Gulf Stream and a climate change role in driving shifts in species' ranges and altering marine ecosystem dynamics.
Antimicrobial effects of chlorine dioxide in a hospital setting
Authors: Helou Mariana , Mahdi Ahmad , Abou Fayad Antoine , Sleiman Ahmad , Matar Ghassan M. , Zoghbi Sanaa , Madani Tarek , Husni Rola 
Published: December 2023

AbstractChlorine dioxide is a powerful disinfectant with strong antibacterial properties. We conducted a study at different sites of the Lebanese American University Medical Center-Rizk Hospital to determine the efficacy of the ECOM air mask in decreasing the particle load. Air cultures were obtained from three different locations, namely the patients’ elevator, visitors’ elevator and mobile clinic and the number of colonies grown on each type of agar was determined. We also measured particle counts at the three sites both at baseline and after placement of the ECOM air mask. After 7 days of ECOM air mask use, the numbers of colonies grown on all types of media was decreased by 20–100% versus the baseline values. The counts of particles of different diameters (0.3, 0.5 and 5 µm) were decreased at all three sampled sites. This study highlighted the efficacy of the ECOM air mask. The utility of the gaseous form of ClO2 as an antiseptic in the hospital setting appears promising.
Serosurvey and associated risk factors for Neospora caninum infection in Egyptian water buffaloes (Bubalus bubalis)
Authors: Alshammari Ayed , Marzok Mohamed , Gattan Hattan S. , Salem Mohamed , Al-Jabr Omar A. , Selim Abdelfattah 
Published: December 2023

AbstractNeosporosis is a parasitic disease that causes reproductive disorders in animals, making it a barrier to maximum efficiency. The purpose of this study was to determine the seroprevalence ofNeosporacaninum(N.caninum) antibodies in water buffaloes from four governorates in northern Egypt. A commercial indirect-ELISA test was used to detect antibodies againstN.caninumin the serum of 450 water buffaloes. The total seroprevalence ofN.caninumin water buffaloes from Egypt was 31.3%, and the highest prevalence was observed in Gharbia governorate. The identified risk factors forN.caninuminfections in water buffaloes were sex (OR = 1.96, 95%CI: 1.22−4.17), buffaloes more than 4 years of age ( OR = 5.80, 95%CI: 2.26−14.86), abortion in second trimester (OR = 16.48, 95%%CI: 2.99−34.03), history of abortion (OR = 3.45, 95%CI: 1.58−7.52) and contact with dogs (OR = 2.55, 95%CI: 1.51−4.32). Thus, more studies are needed to determine the role of buffaloes in the epidemiology of neosporosis in Egypt.
Synthesis and in-vitro anti-proliferative with antimicrobial activity of new coumarin containing heterocycles hybrids
Authors: Abdelaziz Eman , El-Deeb Nehal M. , Zayed Mervat F. , Hasanein Asmaa Mohammed , El Sayed Ibrahim El-T. , Elmongy Elshaymaa I. , Kamoun Elbadawy A. 
Published: December 2023

AbstractA series of new coumarin-N-heterocyclic hybrids, coumarin-quinolines 7a–e, coumarin-acridines 10b,c and coumarin-neocryptolepines 13b,c were synthesized and evaluated for their anticancer and antimicrobial activities. The structures of all synthesized hybrids were confirmed by FT-IR, 1H-NMR, 13C-NMR, and MS spectrometry. The anti-proliferative activity of hybrids 7a–e, 10c and 13c were bio-evaluated using MTT-assay against colon (CaCo-2), lung (A549), breast (MDA-MB-231), and hepatocellular carcinoma (HepG-2) human cancer cell lines using doxorubicin as a reference drug. The results demonstrated that, all hybrids displayed moderate to good anti-proliferative activity against the cell lines. The most active hybrids were 7a–d and 10c against CaCo-2 cancer cell line with IC50: 57.1, 52.78, 57.29, 51.95 and 56.74 µM, and selectivity index 1.38, 1.76, 2.6, 1.96 and 0.77; respectively. While, 7a,d were potent against A549 cancer cell line with IC50: 51.72, 54.8 µM and selectivity index 1.5, 0.67; respectively. Moreover, 7c showed the most potency against MDA-MB-231 cancer cell line with IC50: 50.96 µM and selectivity index 2.20. Interestingly, docking results revealed that binding energy of the current compounds showed marked affinity values ranging from -6.54 to -5.56 kcal with interactions with the reported key amino acid SER 79. Furthermore, the antimicrobial activity of the synthesized hybrids 7a–e, 10b,c, 13b and 13c were evaluated against Gram‐positive and Gram‐negative bacterial and fungal strains. The hybrids 10b, 13b, 10c, and 13c exhibited broad-spectrum antibacterial activity against E.coli, S. mutans, and S. aureus with MIC from 3.2 to 66 µM, this hybrids also displayed antifungal activity against C. albicans with MIC values ranging from 0.0011 to 29.5 µM. In-silico investigation of the pharmacokinetic properties indicated that tested hybrids had high GI absorption, low Blood Brain Barrier (BBB) permeability in addition to cell membrane penetrability.
Immobilization of Aspergillus oryzae tyrosine hydroxylase on ZnO nanocrystals for improved stability and catalytic efficiency towards L-dopa production
Authors: Khalid Ansa , Ali Sikander , Rukhma  , Jahangeer Muhammad , Sarwar Abid , Nelofer Rubina , Aziz Tariq , Alharbi Metab , Alasmari Abdullah F. , Albekairi Thamer H. 
Published: December 2023

AbstractThe current study focuses on the submerged fermentation of tyrosine hydroxylase (TH) from Aspergillus oryzae IIB-9 and its immobilization on zinc oxide nanocrystals (ZnO-NPs) for increased L-dopa production. The volume of Vogel’s medium (75 ml), period of incubation (72 h), initial pH (5.5), and size of inoculum (1.5 ml) were optimal for maximum TH activity. The watch glass-dried (WG) and filter paper-dried (FP) ZnO-NPs were prepared and characterized using analytical techniques. The UV–Vis spectra revealed 295 and 285 nm absorption peaks for WG-ZnO-NPs and FP-ZnO-NPs dispersed in isopropanol. X-ray diffraction analysis confirmed the crystalline nature of ZnO-NPs. FTIR spectra band from 740 to 648.1/cm and 735.8/cm to 650.1/cm showed the stretching vibrations of WG-ZnO-NPs and FP-ZnO-NPs, respectively. The particle size of ZnO-NPs observed by scanning electron microscopy (SEM) images was between 130 and 170 nm. Furthermore, the stability of immobilized TH on ZnO-NPs was determined by varying the incubation period (10 min for WG-NPs and 15 min for FP-NPs) and temperature (45 °C and 30 °C for WG and FP-NPs, respectively). Incubating enzymes with various copper, iron, manganese, and zinc salts studied the catalytic efficiency of TH. Immobilization of TH on ZnO-NPs resulted in an 11.05-fold increase in TH activity, thus enhancing stability and catalytic efficiency.
Verbal lie detection using Large Language Models
Authors: Loconte Riccardo , Russo Roberto , Capuozzo Pasquale , Pietrini Pietro , Sartori Giuseppe 
Published: December 2023

AbstractHuman accuracy in detecting deception with intuitive judgments has been proven to not go above the chance level. Therefore, several automatized verbal lie detection techniques employing Machine Learning and Transformer models have been developed to reach higher levels of accuracy. This study is the first to explore the performance of a Large Language Model, FLAN-T5 (small and base sizes), in a lie-detection classification task in three English-language datasets encompassing personal opinions, autobiographical memories, and future intentions. After performing stylometric analysis to describe linguistic differences in the three datasets, we tested the small- and base-sized FLAN-T5 in three Scenarios using 10-fold cross-validation: one with train and test set coming from the same single dataset, one with train set coming from two datasets and the test set coming from the third remaining dataset, one with train and test set coming from all the three datasets. We reached state-of-the-art results in Scenarios 1 and 3, outperforming previous benchmarks. The results revealed also that model performance depended on model size, with larger models exhibiting higher performance. Furthermore, stylometric analysis was performed to carry out explainability analysis, finding that linguistic features associated with the Cognitive Load framework may influence the model’s predictions.
Divergent preference functions generate directional selection in a jumping spider
Authors: Castilho Leonardo Braga 
Published: December 2023

AbstractSexual selection has long been thought to promote speciation, but this possibility still remains a topic of controversy. Many theoretical models have been developed to understand the relationship between sexual selection and speciation, but such relationship seems complex and sexual selection has also been argued to prevent speciation in many scenarios. Here, I model for the first time the tendency of speciation due to sexual selection using realistic model parameters input collected from an existing species, the jumping spider Hasarius adansoni. I show that, even though the species has substantial female variance in preference (the model typically thought to link sexual selection to speciation), when realistic parameters are input in the model, it predicts directional selection, rather than disruptive selection. I propose that including realistic parameters in speciation models is a new tool that will help us understand how common sexual selection helps or hinders speciation in the real world.
Combining chromosomal microarray and clinical exome sequencing for genetic diagnosis of intellectual disability
Authors: Kim Jaewon , Lee Jaewoong , Jang Dae-Hyun 
Published: December 2023

AbstractDespite the current widespread use of chromosomal microarray analysis (CMA) and exome/genome sequencing for the genetic diagnosis of unexplained intellectual disability (ID) in children, gaining improved diagnostic yields and defined guidelines remains a significant challenge. This is a cohort study of children with unexplained ID. We analyzed the diagnostic yield and its correlation to clinical phenotypes in children with ID who underwent concurrent CMA and clinical exome sequencing (CES). A total of 154 children were included (110 [71.4%] male; mean [SD] age, 51.9 [23.1] months). The overall diagnosis yield was 26.0–33.8%, with CMA contributing 12.3–14.3% and CES contributing 13.6–19.4%, showing no significant difference. The diagnostic rate was significantly higher when gross motor delay (odds ratio, 6.69; 95% CI, 3.20–14.00; P < 0.001), facial dysmorphism (odds ratio, 9.34; 95% CI 4.29–20.30; P < 0.001), congenital structural anomaly (odds ratio 3.62; 95% CI 1.63–8.04; P = 0.001), and microcephaly or macrocephaly (odds ratio 4.87; 95% CI 2.05–11.60; P < 0.001) were presented. Patients with only ID without any other concomitant phenotype (63/154, 40.9%) exhibited a 6.3–11.1% diagnostic rate.
Single-cell morphological and transcriptome analysis unveil inhibitors of polyploid giant breast cancer cells in vitro
Authors: Zhou Mengli , Ma Yushu , Chiang Chun-Cheng , Rock Edwin C. , Butler Samuel Charles , Anne Rajiv , Yatsenko Svetlana , Gong Yinan , Chen Yu-Chih 
Published: December 2023

AbstractConsiderable evidence suggests that breast cancer therapeutic resistance and relapse can be driven by polyploid giant cancer cells (PGCCs). The number of PGCCs increases with the stages of disease and therapeutic stress. Given the importance of PGCCs, it remains challenging to eradicate them. To discover effective anti-PGCC compounds, there is an unmet need to rapidly distinguish compounds that kill non-PGCCs, PGCCs, or both. Here, we establish a single-cell morphological analysis pipeline with a high throughput and great precision to characterize dynamics of individual cells. In this manner, we screen a library to identify promising compounds that inhibit all cancer cells or only PGCCs (e.g., regulators of HDAC, proteasome, and ferroptosis). Additionally, we perform scRNA-Seq to reveal altered cell cycle, metabolism, and ferroptosis sensitivity in breast PGCCs. The combination of single-cell morphological and molecular investigation reveals promising anti-PGCC strategies for breast cancer treatment and other malignancies.
Summer insolation controlled movements of Intertropical Convergence Zone during last glacial cycle in northern South America
Authors: Ramirez V. M. , Cruz F. W. , Vuille M. , Novello V. F. , Stríkis N. M. , Cheng H. , Zhang H. W. , Bernal J. P. , Du W. J. , Ampuero A. , Deininger M. , Chiessi C. M. , Tejedor E. , Campos J. L. , Ait Brahim Y. , Edwards R. L. 
Published: December 2023

AbstractA paradigm in paleoclimatology holds that shifts in the mean position of the Intertropical Convergence Zone were the dominant climatic mechanism controlling rainfall in the tropics during the last glacial period. We present a new paleo-rainfall reconstruction based on speleothem stable oxygen isotopes record from Colombia, which spans most of the last glacial cycle. The strength and positioning of the Intertropical Convergence Zone over northern South America were more strongly affected by summer insolation at high northern latitudes than by local insolation during the last glacial cycle, resulting in an antiphased relationship with climate in the Cariaco Basin. Our data also provide new insight into how orbital forcing amplified/dampened Intertropical Convergence Zone precipitation during millennial-scale events. During Greenland Stadial events, the Intertropical Convergence Zone was positioned close to the latitude of El Peñon, as expressed by more negative δ18O values. Greenland Interstadial events are marked by relatively high stable oxygen isotope values and reduced rainfall in the El Peñon record, suggesting a northward withdrawal of the Intertropical Convergence Zone. During some Heinrich Stadial events, and especially Heinrich Stadial 1, the Intertropical Convergence Zone must have been displaced away from its modern location near El Peñon, as conditions were very dry at both El Peñon and Cariaco.
NADase CD38 is a key determinant of ovarian aging
Authors: Yang Qingling , Chen Wenhui , Cong Luping , Wang Mengchen , Li Hui , Wang Huan , Luo Xiaoyan , Zhu Jing , Zeng Xinxin , Zhu Zhenye , Xu Yining , Lei Min , Zhao Yanqing , Wei Chenlu , Sun Yingpu 
Published: December 2023

AbstractThe ovary ages earlier than most other tissues, yet the underlying mechanisms remain elusive. Here a comprehensive analysis of transcriptomic landscapes in different organs in young and middle-aged mice revealed that the ovaries showed earlier expression of age-associated genes, identifying increased NADase CD38 expression and decreased NAD+ levels in the ovary of middle-aged mice. Bulk and single-cell RNA sequencing revealed that CD38 deletion mitigated ovarian aging, preserving fertility and follicle reserve in aged mice by countering age-related gene expression changes and intercellular communication alterations. Mechanistically, the earlier onset of inflammation induced higher expression levels of CD38 and decreased NAD+ levels in the ovary, thereby accelerating ovarian aging. Consistently, pharmacological inhibition of CD38 enhanced fertility in middle-aged mice. Our findings revealed the mechanisms underlying the earlier aging of the ovary relative to other organs, providing a potential therapeutic target for ameliorating age-related female infertility.
How does humble leadership influence employee improvisation? A motivational perspective
Authors: Sun Liuqi , Huang Chongrong , Wu Zhifan , Li Chengyan 
Published: December 2023

AbstractIn the era of variability, uncertainty, complexity, and ambiguity, organizations must improvise to deal with emergencies. Drawing on the proactive motivation model, we explored the connection between humble leadership and employees’ improvisation, and its mechanism and boundary conditions. The participants were sourced from various enterprises located in Shanghai, Shandong, Heilongjiang, Hainan, and other regions. Through a scenario-based simulation experiment (N = 91) and a questionnaire survey (N = 217), we derived five key findings. First, humble leadership positively affects employee improvisation. Employees’ positive emotions mediated the relationship, while both positive employee emotions and leader–member exchanges play a chain-mediating role. Moreover, power distance orientation negatively moderates the promotion effect. Finally, the indirect effect of humble leadership on improvisation via positive employee emotion is stronger for employees with low power distance orientations. Our study primarily focuses on individual-level improvisation, which enriches the knowledge of the connection between leadership style and improvised behaviors while also expanding upon the proactive motivation model framework. Additionally, practical insights are provided for promoting improvisation.
OctoShaker: A versatile robotic biomechanical agitator for cellular and organoid research
Authors: Huang Yan , Lee Soojung , Liu Wenhao , Takayama Shuichi , Jia Shu 
Published: December 2023

Mechanical forces have increasingly been recognized as a key regulator in the fate of cellular development and functionality. Different mechanical transduction methods, such as substrate stiffness and magnetic bead vibration, have been experimented with to understand the interaction between the biophysical cues and cellular outcome. In the exploration and utilization of the intrinsic cellular mechanism, bio-shakers, traditionally invented for stirring liquid, have garnered more interest as a tool to provide precise mechanical stimuli to aid in this study. Nonetheless, despite the usefulness of current bio-shaking technology, each type of shaker often offers a single mode of motion, insufficient for generating complex force dynamics needed to resemble the actual physical condition that occurs inside living organisms. In this study, we present OctoShaker, a robotic instrument capable of creating a multitude of motions that could be sequenced or programmed to mimic sophisticated hemodynamics in vivo. We demonstrated the programmed motion of circular convection and investigated its influence on micro-particle distribution in 96-well culture microplates. Biological samples, including HeLa cells and organoids, were tested, and unique resultant patterns were observed. We anticipate the open-source dissemination of OctoShaker in diverse biological applications, encompassing biomechanical studies for cellular and organoid research, as well as other disciplines that demand dynamic mechanical force generation.
Determiners of social inclusion and exclusion in the dementia context
Authors: Wray Alison , Bergström Axel 
Published: December 2023

Abstract
People living with a dementia and their family carers are at high risk of being excluded from the contact,
activities, information and services that help them remain resilient. Using interview data from family dementia carers, this
article explores the sources of enablement and inhibition in accessing these aspects of social inclusion. Carers and those living
with a dementia are found to inhibit and enable social inclusion for themselves, each other, and other carers. However, carers
attribute most agency to the external environment: what is provided and how easy it is to access, along with the attitudes and
beliefs of others. Poor communication surfaces as an impediment to social inclusion, with carers often left trying to bridge
communicative gaps despite limited knowledge and self-confidence.
Conversation practices that foster or hinder inclusivity during interactions involving persons with dementia
Authors: Stickle Trini 
Published: December 2023

Abstract
This article highlights co-participant strategies during conversations involving participants diagnosed with dementia that encourage continued, productive interaction fostering inclusivity. The conversation excerpts illustrate positive results when non-impaired co-participants respond to impaired syntax as though it makes sense or as if it completes a coherent syntactic–semantic action. Helpful co-participants yield control of conversations, rather than issuing corrections; demonstrate an acuity for when to divert topics; and acknowledge their co-participants’ concerns. In contrast, non-inclusive strategies include overuse of questions, overcorrecting, minimizing dementia symptoms, or dismissing co-participants’ concerns, which frequently result in persons with dementia resisting or withdrawing from conversations, often accompanied by displays of anger. The goal is to raise awareness of strategies which promise an increase in and longer duration of interactions, decrease loneliness, and increase health and emotional outcomes of older persons.
Partículas discursivas y prosodia
Authors: Hidalgo Antonio , Briz Gómez Antonio 
Published: December 2023

Abstracta¿Sabes?y¿entiendes?son partículas discursivas de control del contacto que desempeñan una función fático-apelativa, en mayor o menor medida fática o apelativa, a la que se añade ya sea como valor principal o subsidiario un valor modalizador, intensificador y, ocasionalmente, atenuante. En su descripción se subraya a menudo la importancia de la prosodia como rasgo determinante para establecer su función, pero son pocos los estudios que analizan de forma exhaustiva dicho comportamiento prosódico. Nuestra hipótesis de partida es que la diversidad funcional de estos marcadores va asociada a su versatilidad prosódica en virtud, entre otros factores, de la posición que ocupan en el discurso, atendiendo al modelo de unidades del discurso oral del Grupo Val.Es.Co. (Grupo Val.Es.Co. 2014). Analizamos así su realización prosódica para poder llegar a establecer tendencias de regularidad que enriquezcan su descripción: se trata de una propuesta que aúna los criterios funcional, posicional y prosódico.