bluuebunny/crossref_metadata_2025_split huggingface dataset:
Level of and Changes in Perceived Work Ability Among Partial Disability Pensioners and the Risk of Full Disability Pension—A Register-Linked Cohort Study
Authors: Wallius Mari-Anne , Lallukka Tea , Leinonen Taina , Remes Jouko , Ervasti Jenni 
Published: December 2023

Abstract
Purpose
To examine how the level of perceived work ability and its changes over time are associated with the risk of full disability pension (DP) among those receiving partial DP.

Methods
We retrieved survey data on perceived work ability and covariates (sociodemographic factors and health behaviors) from a cohort study of Finnish public sector employees at two time points: 2008 and 2012 and linked them with register data on DP obtained from the Finnish Centre for Pensions up to the end of 2018. Participants had begun receiving partial DP in 2008 and responded to either the 2008 survey (n = 159) or both surveys (n = 80). We used Cox regression for the analyses.

Results
During the follow-up, 61 (38%) of those receiving partial DP transitioned to full DP. Those with perceived poor work ability were at a higher risk of full DP (HR 1.93; 95% CI 1.11–3.38) than those with at least moderate work ability, after adjustment for covariates. During four years of receiving partial DP, perceived work ability decreased among 36% of the participants, and remained unchanged or improved among 64%. Change in work ability was not associated with a risk of full DP.

Conclusion
Among those receiving partial DP, perceived poor work ability was a risk factor for full DP. Our findings highlight the importance of monitoring the level of perceived work ability of those receiving partial DP to enable identifying individuals at an increased risk of full DP.
Genomic insights and advanced machine learning: characterizing autism spectrum disorder biomarkers and genetic interactions
Authors: Nahas Laila Dabab , Datta Ankur , Alsamman Alsamman M. , Adly Monica H. , Al-Dewik Nader , Sekaran Karthik , Sasikumar K , Verma Kanika , Doss George Priya C , Zayed Hatem 
Published: December 2023

AbstractAutism Spectrum Disorder (ASD) is a complex neurodevelopmental condition characterized by altered brain connectivity and function. In this study, we employed advanced bioinformatics and explainable AI to analyze gene expression associated with ASD, using data from five GEO datasets. Among 351 neurotypical controls and 358 individuals with autism, we identified 3,339 Differentially Expressed Genes (DEGs) with an adjusted p-value (≤ 0.05). A subsequent meta-analysis pinpointed 342 DEGs (adjusted p-value ≤ 0.001), including 19 upregulated and 10 down-regulated genes across all datasets. Shared genes, pathogenic single nucleotide polymorphisms (SNPs), chromosomal positions, and their impact on biological pathways were examined. We identified potential biomarkers (HOXB3, NR2F2, MAPK8IP3, PIGT, SEMA4D, and SSH1) through text mining, meriting further investigation. Additionally, ‎we shed light on the roles of RPS4Y1 and KDM5D genes in neurogenesis and neurodevelopment. Our analysis detected 1,286 SNPs linked to ASD-related conditions, of which 14 high-risk SNPs were located on chromosomes 10 and X. We highlighted potential missense SNPs associated with FGFR inhibitors, suggesting that it may serve as a promising biomarker for responsiveness to targeted therapies. Our explainable AI model identified the MID2 gene as a potential ASD biomarker. This research unveils vital genes and potential biomarkers, providing a foundation for novel gene discovery in complex diseases.
A simple and rapid assay of lysosomal-targeting CDy6 for long-term real-time viability assessments in 2D and 3D in vitro culture models
Authors: Kang Chanhan , Yun Won-Soo , Jeong Yun-Mi 
Published: December 2023

AbstractCDy6, a BODIPY-derived compound, is used to label lysosomes and visualize proliferating cells. However, its effectiveness in long-term, real-time cell viability assays using 2D or 3D cell culture models is unclear. We evaluated the suitability of CDy6 by assessing cell health using human keratinocyte and fibroblast cell lines in both models. Cells were stained with CDy6 or other dyes and fluorescent images were obtained with confocal microscopy. CLV extracts derived from CDy6-stained HaCaT cells were also dissolved with DMSO and analyzed using a spectrometer. Furthermore, we added CDy6-stained collagen hydrogels to CCD-986sk cells, loaded them into a frame construction to establish a 3D dermal layer for long-term culture, and analyzed the status of the CLVs. The CLV method, also measured using a spectrometer, yielded results similar to MTT assay for validating viability. In contrast to calcein AM staining, the CLV method allows for both absorbance measurement and imaging under short-term and long-term culture conditions with less cytotoxicity. In conclusion, the CLV method provides a simple and sensitive tool for assessing the status of live cells in 2D and 3D in vitro cell culture models and can be used as an alternative to animal testing, such as with 3D artificial skin models.
Investigation of the effect of carnitine on cerebral vasospasm in experimental subarachnoid hemorrhage model
Authors: Resitoglu Gokhan , Akgun Mehmet Yigit , Ates Ozkan , Oztanir Mustafa Namik 
Published: December 2023

AbstractThe vasospasm, which develops after subarachnoid hemorrhage (SAH), is an unenlightened table in terms of etiology and results. It is usually associated with decreased perfusion, which is associated with decreased blood flow distal to the affected artery and can be demonstrated radiologically. Acetyl-L-carnitine (ALCAR) can be found in brain tissue and easily crosses the blood–brain barrier. Therefore, in this study, we aimed to investigate the therapeutic efficacy of ALCAR, which is an effective antioxidant amine, on vasospasm development after experimental SAH. In our study, 35 adults male Wistar RATs weighing between 235–250 g were used. These RATs were divided into five groups with n = 7. Group 1 Control group, Group 2 SAH + SF (carrier solution), Group 3 SAH + ALCAR 50 mg\kg intraperitoneally, Group 4 SAH + ALCAR 100 mg\kg intraperitoneally and Group 5 SAH. Subarachnoid hemorrhage was induced by giving autologous arterial blood to the cisterna magna of the animals in groups 2, 3, 4, and 5. At 0.-12.- 24.- 36.- 48.- 60. and 72. h, Group 2 was injected with SF, Group 3 with intraperitoneally ALCAR 50 mg\kg, and Group 4 with intraperitoneally ALCAR 100 mg\kg, respectively. Following perfusion and fixation, the animals were subjected to a wide craniectomy, and the brain, cerebellum, and brain stems were removed globally. Then, sections were taken from the basilar arteries of all animals and photographed at 40X magnification. Basilar artery lumen cross-sectional areas, basilar artery areas, and wall thicknesses were measured from these sections. The basilar artery lumen cross-sectional area was found to be significantly larger in the groups in which SAH was formed and ALCAR 50 mg\kg and ALCAR 100 mg\kg were given compared to the group with only SAH and SAH + SF (p = 0.0408). Basilar artery wall thickness increased in all groups except the control group (p < 0.05). In light of all these findings, it was concluded in our study that Carnitine was effective in the resolution of vasospasm in the experimental SAH model.
Identification of neutrophil extracellular traps and crosstalk genes linking inflammatory bowel disease and osteoporosis by integrated bioinformatics analysis and machine learning
Authors: Xu Gang , Zhang Wanhao , Yang Jun , Sun Na , Qu Xiaochen 
Published: December 2023

AbstractMusculoskeletal deficits are among the most common extra-intestinal manifestations and complications of inflammatory bowel disease (IBD). This study aimed to identify crosstalk genes between IBD and osteoporosis (OP) and potential relationships between crosstalk and neutrophil extracellular traps (NETs)-related genes. Three common hub genes from different compared groups are actually the same, namely HDAC6, IL-8, and PPIF. ROC showed that the combined diagnostic value of HDAC6, IL-8, and PPIF was higher than each of the three key hub genes. Immune infiltration results showed that HDAC6 and IL-8 key genes negatively correlated with CD65 bright natural killer cells. USF1 was the common upstream TFs between HDAC6 and PPIF, and MYC was the common upstream TFs between IL-8 and PPIF in RegNetwork. Taken together, this study shows a linked mechanism between IBD and OP via NETs and crosstalk genes. These findings may show light on better diagnosis and treatment of IBD complicated with OP.
Optogenetic induction of caspase-8 mediated apoptosis by employing Arabidopsis cryptochrome 2
Authors: Mo Weiliang , Su Shengzhong , Shang Ruige , Yang Liang , Zhao Xuelai , Wu Chengfeng , Yang Zhenming , Zhang He , Wu Liuming , Liu Yibo , He Yun , Zhang Ruipeng , Zuo Zecheng 
Published: December 2023

AbstractApoptosis, a programmed cell death mechanism, is a regulatory process controlling cell proliferation as cells undergo demise. Caspase-8 serves as a pivotal apoptosis-inducing factor that initiates the death receptor-mediated apoptosis pathway. In this investigation, we have devised an optogenetic method to swiftly modulate caspase-8 activation in response to blue light. The cornerstone of our optogenetic tool relies on the PHR domain of Arabidopsis thaliana cryptochrome 2, which self-oligomerizes upon exposure to blue light. In this study, we have developed two optogenetic approaches for rapidly controlling caspase-8 activation in response to blue light in cellular systems. The first strategy, denoted as Opto-Casp8-V1, entails the fusion expression of the Arabidopsis blue light receptor CRY2 N-terminal PHR domain with caspase-8. The second strategy, referred to as Opto-Casp8-V2, involves the independent fusion expression of caspase-8 with the PHR domain and the CRY2 blue light-interacting protein CIB1 N-terminal CIB1N. Upon induction with blue light, PHR undergoes aggregation, leading to caspase-8 aggregation. Additionally, the blue light-dependent interaction between PHR and CIB1N also results in caspase-8 aggregation. We have validated these strategies in both HEK293T and HeLa cells. The findings reveal that both strategies are capable of inducing apoptosis, with Opto-Casp8-V2 demonstrating significantly superior efficiency compared to Opto-Casp8-V1.
LiO-t-Bu/CsF-Mediated Formal Hydrolysis of Trifluoromethyl Arenes
Authors: Shigeno Masanori , Kiriyama Moe , Izumi Koki , Sasaki Keita , Sasamoto Ozora , Nozawa-Kumada Kanako , Kondo Yoshinori 
Published: December 2023

AbstractThis study investigates the formal hydrolysis of trifluoromethyl arenes without deprotonation functionality utilizing a combined Brønsted base system comprising LiO-t-Bu and CsF. The reaction conditions were optimized using 4-(trifluoromethyl)biphenyl as the model substrate, achieving a 94% yield with LiO-t-Bu/CsF. The scope of the substrate was explored, demonstrating the applicability of the system to various functionalities, such as (hetero)aryl, tert-butyl, methyl, amide, and alkenyl moieties. Mechanistic insights suggest a single electron transfer process.
Magnesiation of Alkyl Fluorides Catalyzed by Rhodium–Aluminum Bimetallic Complexes
Authors: Nakao Yoshiaki , Fujii Ikuya , Higo Ryota , Semba Kazuhiko 
Published: December 2023

AbstractSince the pioneering work by Grignard in 1900, organomagnesium reagents, the so-called Grignard reagents, have been indispensable in organic synthesis. Alkyl Grignard reagents are usually prepared from the corresponding alkyl iodides, bromides, or chlorides with Mg, whereas alkyl fluorides are not viable substrates under conventional conditions due to the high stability of the C–F bonds. We report that Al–Rh bimetallic complexes catalyze the magnesiation of C(sp3)–F bonds of alkyl fluorides using easy-to-handle Mg powder. The present conditions can accommodate primary, secondary, or tertiary alkyl fluorides to afford the corresponding alkylmagnesium reagents, which can be successfully converted into various functionalities.
Systematic Review and Meta-analysis of Randomized Controlled Trials on Manual Therapy for the Treatment of Gastroesophageal Reflux Disease
Authors: Yan Mengdie , Shi Yunzhou , Liu Yanqin , Shi Yue , Zhang Mengzhu , Chen Dongnan , Ye Senlin , Li Shiyin , Feng Yue 
Published: December 2023

Abstract
Objective The objective of our study was to systematically evaluate the clinical efficacy and safety of manual therapy in the treatment of gastroesophageal reflux disease (GERD), providing a reference value for clinical decision-making.
Method Studies of randomized controlled trials (RCTs) evaluating the efficacy of manual therapy in patients with GERD were searched through Chinese National Knowledge Infrastructure, Wanfang, VIP China Science and Technology Journal Database, China Biology Medicine Database, PubMed, The Cochrane Library, OVID Medline, and Embase. Two researchers independently reviewed the literature, extracted data, and performed a risk of bias analysis using the Cochrane risk of bias assessment tool, and conducted meta-analysis analysis and publication bias evaluation, the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) tool 26 was used to rate the caliber of the data in this meta-analysis.
Results This study included 11 RCTs. Meta-analysis showed that the manual therapy group had a higher total effective rate (odds ratio [OR] = 4.63, 95% confidence interval [CI; 3.01, 7.14], p < 0.00001) and better Reflux Disease Questionnaire scores {weighted mean difference (WMD) = −1.59, 95% CI [−2.85, −0.33], p = 0.01} than the control group. The subgroup analysis showed significant differences in improving the total effective rate in manual therapy versus Western medicine, manual therapy versus Chinese medicine decoction, manual therapy + Western medicine versus Western medicine, and manual therapy + conventional treatment versus conventional treatment groups. Among the 11 trials, 5 reported adverse events, and all RCTs had the possibility of publication bias. Subgroup analysis shows that the differences in age could significantly influence heterogeneity; The GRADE analysis revealed that the overall quality of evidence for all outcome indicators was low and did not support our recommendation for the outcome.
Conclusion Manual therapy is more effective than medication therapy alone in relieving GERD symptoms. Furthermore, conventional therapy combined with manual therapy was found to be even more effective. Hence, it is crucial to consider these findings when applying manual therapy to GERD patients to enhance treatment outcomes. Future studies must address issues such as study quality, treatment duration, and generalizability.
Correlation between the Distribution of Traditional Chinese Medicine Syndromes and Molecular Types of Breast Cancer in Perichemotherapy Period
Authors: Meng Bingxin , Cheng Xufeng , Liu Qi , Zhao Huiduo , Wang Beibei , Xu Liuyan 
Published: December 2023

Abstract
Objectives The objective of this study was to explore the correlation between the distribution of traditional Chinese medicine (TCM) syndromes and molecular types of breast cancer in the perichemotherapy period.
Methods A total of 325 cases with perichemotherapy breast cancer was classified according to syndrome differentiation in TCM , and R × C table χ2 test was used to examine and analyze the relationship between TCM syndromes and molecular types of breast cancer in the perichemotherapy period.
Results (1) In the early stage of chemotherapy, there was no significant difference in the distribution of different TCM syndromes among molecular types, mainly liver depression syndrome and liver depression and phlegm coagulation syndrome (p > 0.05). (2) In the middle stage of chemotherapy, there were significant differences in the distribution of spleen deficiency and phlegm-dampness syndrome among HER-2 positive (HR positive), HER-2 positive (HR negative), and Luminal A type, Luminal B type (HER-2 negative), and triple-negative type (p < 0.01). (3) After chemotherapy, there were significant differences in the distribution of spleen and kidney yang deficiency syndrome and marrow sea insufficiency syndrome among HER-2 positive (HR negative), triple-negative type, and HER-2 positive (HR positive), Luminal A type, Luminal B type (HER-2 negative), and triple-negative type (p < 0.01).
Conclusion (1) In the middle stage of chemotherapy, HER-2 positive (HR positive) and HER-2 positive (HR negative) are more likely to show spleen deficiency and phlegm-dampness syndrome than other molecular types. (2) In the late stage of chemotherapy, the HER-2 positive (HR negative) and triple-negative type is more likely to show spleen-kidney yang deficiency syndrome than other molecular types, and the triple-negative type is more likely to show marrow sea insufficiency syndrome than other molecular types.
A multimodal flow reactor for photocatalysis under atmospheric conditions
Authors: Garcia-Esparza Angel T. , Qureshi Muhammad , Skoien Dean , Hersbach Thomas J. P. , Sokaras Dimosthenis 
Published: December 2023

Photocatalysis is a promising concept for the direct conversion of solar energy into fuels and chemicals. The design, experimental protocol, and performance of a multimodal and versatile flow reactor for the characterization of powdered and immobilized photocatalysts are herein presented. Ultimately, this instrument enables rigorous evaluation of photocatalysis performance metrics. The apparatus quantifies transient gas-phase reaction products via online real-time gas analyzer mass spectrometry (RTGA-MS). For H2, the most challenging gas, the photocatalytic system’s RTGA-MS gas detection sensitivity spans over three orders of magnitude and can detect down to tens of parts per million under atmospheric conditions. Using Pt nanoparticles supported on anatase TiO2 photocatalyst via wet impregnation, the instrument’s capability for the characterization of photocatalytic H2 evolution is demonstrated, resulting in an apparent quantum yield (AQY) of 48.1% ± 0.9% at 320 nm, 45.7% ± 0.3% at 340 nm and 31% ± 1% at 360 nm. The photodeposition of Pt on anatase TiO2 was employed to demonstrate the instrument’s capability to track the transient behavior of photocatalysts, resulting in an improved 55% ± 2% AQY for H2 evolution at 340 nm from aqueous methanol. This photocatalytic instrument enables systematic study of a wide variety of photocatalytic reactions such as water splitting and CO2 reduction to valuable C2+ fuels and chemicals.
ConvGRU-MHM: a CNN GRU-enhanced MHM for mitigating GNSS multipath
Authors: Tong Runfa , Liu Chao , Tao Yuan , Wang Xiangyang , Sun Jingqiang 
Published: December 2023

Abstract
In high-precision global navigation satellite system (GNSS) short-baseline positioning, multipath is the main source of errors. If the station environment is quasi-static, repeat periods of satellites can be utilized to generate time- or space-dependent multipath models to mitigate multipaths. However, two general problems are associated with the multipath models constructed based on satellite mechanics: (1) an accuracy decrease occurs when the above models are applied to multipath mitigation over a long time-span; (2) when constructing the spatial and temporal grids of the satellite-based spatially dependent multipath model, it is challenging to balance computational efficiency and spatial resolution. We propose a convolutional neural network-gated recurrent unit enhanced multipath hemispherical map (ConvGRU-MHM) in the observational domain to address these problems. The proposed method directly mines the deep features of elevation, azimuth angle, and multipath and the mapping relationship between these to establish a real-time prediction model. The predicted multipath is obtained and returned to the observation equation for multipath mitigation when the real-time position of the satellite is placed in the pre-trained model. We compared the multipath mitigation performance of sidereal filtering and a MHM with that of the ConvGRU-MHM to demonstrate the advantages of the proposed method. The experimental results are as follows: (1) in the short time-span (first 20 d), the mean accuracy improvements of the ConvGRU-MHM in the E/N/U direction performed better than those of the SF and MHM; and (2) in the long-term time (after 50 d), the mean accuracy improvements of the ConvGRU-MHM in the E/N/U direction are higher than that of the SF and MHM by 10%–20%. As a lightweight model, the ConvGRU-MHM can effectively improve the measurement accuracy of GNSS real-time monitoring in fields, such as deformation monitoring and seismic research.
Two-photon Production in Low-velocity Shocks
Authors: Kulkarni S. R. , Shull J. Michael 
Published: December 2023

Abstract
The Galactic interstellar medium abounds in shocks with low velocities v

s
≲ 70 km s−1. Some are descendants of higher velocity shocks, while others start off at low velocity (e.g., stellar bow shocks, intermediate velocity clouds, spiral density waves). Low-velocity shocks cool primarily via Lyα and two-photon continuum, augmented by optical recombination lines (e.g., Hα), forbidden lines of metals and free-bound emission, free–free emission. The dark far-ultraviolet (FUV) sky, aided by the fact that the two-photon continuum peaks at 1400 Å, makes the FUV band an ideal tracer of low-velocity shocks. GALEX FUV images reaffirm this expectation, discovering faint and large interstellar structure in old supernova remnants and thin arcs stretching across the sky. Interstellar bow shocks are expected from fast stars from the Galactic disk passing through the numerous gas clouds in the local interstellar medium within 15 pc of the Sun. Using the bests atomic data available to date, we present convenient fitting formulae for yields of Lyα, two-photon continuum, and Hα for pure hydrogen plasma in the temperature range of 104–105 K. The formulae presented here can be readily incorporated into time-dependent cooling models as well as collisional ionization equilibrium models.