bluuebunny/crossref_metadata_2025_split huggingface dataset:
RUNX1 facilitates heart failure progression through regulating TGF-β-induced cardiac remodeling
Authors: Qi Peng , Zhai Qian , Zhang Xiquan 
Published: October 2023

Background
Heart failure is caused by acute or chronic cardiovascular diseases with limited treatments and unclear pathogenesis. Therefore, it is urgent to explore new therapeutic targets and reveal new pathogenesis for heart failure.

Methods
We carried out heart failure animal model by transverse aortic arch constriction (TAC) in mice. The left ventricular internal diameter diastole (LVIDd), left ventricular internal diameter systole (LVIDs), and ejection fraction (EF) value were detected using ultrasound and myocardial fibrosis was evaluated by Masson stain assay. Cell apoptosis in myocardial tissues were detected by TUNEL immunofluorescence stain. Signal pathway analysis was performed by dual-luciferase reporter assay and western blot.

Results
Our results showed that inhibition of RUNX1 led to remission of cardiac enlargement induced by TAC in mice. Inhibition of RUNX1 also caused raise of EF and FS value under TAC-induced condition. Besides, RUNX1 inhibition mice showed decreased myocardial fibrosis area under TAC-induced condition. RUNX1 inhibition caused decrease of apoptotic cell rate in myocardial tissues under TAC. Interestingly, we found that RUNX1 could promote the activation of TGF-β/Smads in dual-luciferase reporter assay.

Interpretation
We illustrated that RUNX1 could be considered as a new regulator of myocardial remodeling by activating TGF-β/Smads signaling. Based on this, we concluded that RUNX1 may be developed as a new therapeutic target against heart failure in the future. In addition, this study also provide a new insight for the etiological study on heart failure.
Investigation of Heat Stress among Greenhouse Workers in Chhattisgarh, India
Authors: Tiwari Purvi , Shrivastava A. K. , Dave A. K. 
Published: October 2023

Greenhouse cultivation is spreading as it offers the grower a higher net yield per hectare from high quality crops as it reduces the likelihood of yield decline. The greenhouse is kept at 30-40˚ C with a relative humidity of up to 70%, which could be ideal for plant growth, but is not suitable for workers. Heat exposure in the workplace causes discomfort to the human while working. So, the main goal of conducting this research is to address the extent of heat stress and associated health issues for the greenhouse workers. The study was planned for 2022 in the Raipur district of Chhattisgarh. Prior to the assessment, a questionnaire survey was conducted among 50 greenhouse workers to learn perceptions of heat exposure, heat-related health issues and awareness of heat management. To get the heat exposure air temperature, wet bulb temperature, relative humidity, black globe temperature were measured on hourly basis. The wet bulb globe temperature (WBGT) was measured along with greenhouse workers' physiological parameters to quantify heat exposure. With the help of clock diagram showing Humidex Index (HI). The time period at which farmers most likely to suffer from heat stress was estimated. It was reported that in April, May, June, July, August and September WBGT index reaches varied between 30 and 34°C. According to ISO 7243 the recommended WBGT for comfortable workplace should be less than 31°C. Further study was conducted to get physiological responses of the 21 farm workers in three different type of greenhouses and at three time slots. It resulted that heart rate was maximum i.e. 156 bpm in walk in tunnel at 11 am to 1 pm. The average working heart rate was 20 % more than the open field condition. Hence, the study will help to make the strategies to reduce the exposure of heat stress conditions to the greenhouse workers.
Effect of Plant Growth Regulators on Growth and Yield of Onion (Allium cepa L.)
Authors: Prakash Ram , Singh Rajaneesh , Kumar Virendra , Kumar Munish 
Published: October 2023

A field experiment entitled “Effect of Plant Growth Regulators on Growth and Yield of Onion ( Allium cepa L.)” was conducted during two consecutive years 2019-20 and 2020-21 in rabi season at Agriculture Research Farm, Veer Bahadur Singh Purvanchal University, Jaunpur( U.P) to study the effect of plant growth regulators on growth and quality of onion ( Allium cepa L.) . The trial was laid down in randomized block design (RBD) with three replications and ten treatments viz. T1- Control- Distilled Water, T2. GA3- 50 ppm, T3. GA3- 100 ppm, T4. GA3- 150 ppm, T5- NAA – 50 ppm, T6- NAA- 100 ppm, T7- NAA – 150 ppm, T8- Kinetin- 50 ppm, T9- Kinetin- 100 ppm, T10- 150 ppm . Study results revealed that, there was significant statistical variation in plant height (cm), Number of leaves per plant , collor width ( cm) collar width (cm ) Polar Diamter ( cm ) Equatorial diameter (cm) , Yield per plot, Marketable yield, Total yield ( q/ ha). Basis on the pooled the data study result revealed that maximum plant height(34.05cm at 30 DAT, 51.15 at 60 DAT and 69.90 cm DAT) , number of leaves(6.60 at 30 DAT, 8.90 at 60 DAT and 13.60 at 90 DAT) , Fourth leaf length ( 23.60 cm at 30 DAT, 45.0 cm at 60 DAT and 48. 93 cm at 90 DAT) fourth leaf width( 0.52at 30 DAT, 1.32 at 60 DAT, 1.70 at 90 DAT) , collor width ( 1.24 m), Equatorial diameter ( 6.35), Polar diameter (5.75 cm), Bulb weight ( 41.02) , Bulb yield per plot ( 2.71 kg), marketable yield ( 257.21) and yield per ha. (270.75q).
High Energy Absorption Nacre‐Like Calcium Silicate Hydrate (C‐S‐H) Composite Toward Elastic Cementitious Materials
Authors: Liu Xin , Feng Pan , Agudo Cristina Ruiz , Sun Huiwen , Yu Xiaohan , Avaro Jonathan , Huang Jiale , Hou Dongshuai , Ran Qianping , Hong Jinxiang , Liu Jiaping , Miao Changwen , Cölfen Helmut 
Published: October 2023

AbstractThe low toughness under the tension of cement and concrete materials has been a long‐standing issue for decades and it has become increasingly urgent to address in modern society due to the growing demand for the development of high‐performance and sustainable constructions. Manipulating calcium silicate hydrate (C‐S‐H), the main hydration product of Portland cement, which determines the mechanical properties of cementitious materials, is an attractive method for improving their toughness following a bottom‐up approach. Inspired by the microstructure of nacre, a high energy absorption C‐S‐H‐based composite with a highly ordered structure is fabricated by a designed ternary building block, in which exfoliated montmorillonite provides a template for the nucleation and growth of C‐S‐H generating the “brick”, and polyvinyl alcohol acts as a “mortar” binding all the building blocks together. With the hierarchical toughening strategy explored here, the obtained C‐S‐H composite achieves a remarkable energy absorption of 16.2 ± 2.6 MJ m−3, which surprisingly outperforms the ultra‐high toughness cementitious materials by a factor of 20–60 and is even higher than that of natural nacre and other nacre‐like composites. These findings not only provide valuable insights into enhancing the toughness of cementitious materials but also open possibilities for broadening potential applications of C‐S‐H.
Tunable Built‐In Electric Field in Ru Nanoclusters‐Based Electrocatalyst Boosts Water Splitting and Simulated Seawater Electrolysis
Authors: Chen Wenxia , Wei Wei , Li Fan , Wang Yujie , Liu Meng , Dong Shuai , Cui Jinhai , Zhang Yongya , Wang Rui , Ostrikov Kostya (Ken) , Zang Shuang‐Quan 
Published: October 2023

AbstractThe development of bifunctional electrocatalysts suitable for a wide pH range and seawater splitting under simulated industrial electrolysis conditions is expected to advance practical applications of clean hydrogen energy. Here, the study reports a built‐in electric field approach to assemble heterogeneous Ru nanoclusters (Ru NCs) anchored in P,O co‐doped NiFe layered double hydroxide bifunctional electrocatalysts (Ru NCs/P,O‐NiFe LDH) for overall water splitting. It is revealed that the BEF ensures electron enrichment via unidirectional electron transfer from P,O‐NiFe LDH to Ru nanoclusters due to the difference in the corresponding Fermi levels. The optimized Ru NCs/P,O‐NiFe LDH/NF shows excellent electrocatalytic activity toward hydrogen evolution reaction, oxygen evolution reaction, and overall water splitting in a wide pH range, as well as simulated seawater electrolysis under industry‐relevant conditions. The long‐term catalyst stability under high currents and industrial process temperatures is also demonstrated. Density functional theory calculations further confirm that the active sites at the BEF interface effectively reduce the energy barrier of water electrolysis, thereby facilitating the electrocatalytic processes.
Thermochromic Photonic Crystal Paper with Integrated Multilayer Structure and Fast Thermal Response: A Waterproof and Mechanically Stable Material for Structural‐Colored Thermal Printing
Authors: Zhang Xin , Yin Tian , Ge Jianping 
Published: October 2023

AbstractThermochromic photonic crystals are promising materials for thermal printing due to their unfaded colors under chemical/illuminated environments and the absence of toxic chemicals. However, the slow thermochromic response, the multistep printing procedures, the use of inks or developing liquids, and the requirement of expensive parts in printers limit their applications. Here, a thermochromic polyurethane/hydrophobic‐SiO2 photonic crystal/paraffin (PU/HPO‐SiO2‐PC/Para) film with an integrated multilayer structure is fabricated for all‐solid‐state and single‐step thermal printing that is fully compatible with commercial printers. The fast thermochromic response in milliseconds enables high‐resolution and grayscale printing as the paraffin infiltration and the color change can be finely controlled in a microscale range. The integrated and hydrophobic multilayer structure renders the thermochromic film good stability in daily liquids, which addresses the long‐existing concern of print fading. Meanwhile, the integrated multilayer structure also enhances the mechanical stability when it is deposited on fibrous paper so that people can fold, cut, or staple the thermal papers, and make notes confidently in practical usage.
Surface Termination on Unstable Methylammonium‐based Perovskite Using a Steric Barrier for Improved Perovskite Solar Cells
Authors: Miao Yanfeng , Ren Meng , Wang Haifei , Lu Lei , Liu Xiaomin , Chen Yuetian , Zhao Yixin 
Published: October 2023

AbstractCompared to widely adopted low‐dimensional/three‐dimensional (LD/3D) heterostructure, functional organic cation based surface termination on perovskite can not only realize advantage of defect passivation but also prevent potential disadvantage of the heterostructure induced intercalation into 3D perovskite. However, it is still very challenging to controllably construct surface termination on organic–inorganic hybrid perovskite because the functional organic cations’ substitution reaction is easy to form LD/3D heterostructure. Here, we report using a novel benzyltrimethylammonium (BTA) functional cation with rational designed steric hindrance to effectively surface terminate onto methylammonium lead triiodide (MAPbI3) perovskite, which is composed of the most unstable MA cations. The BTA cation is difficult to form a specific 1.5‐dimensional perovskite of BTA4Pb3I10 by cation substitution with MA cation, which then provides a wide processing window (around 10 minutes) for surface terminating on MAPbI3 films. Moreover, the BTAI surface terminated BTAI‐MAPbI3 shows better passivation effect than BTA4Pb3I10‐MAPbI3 heterojunction. Finally, BTAI surface terminated solar cell (0.085 cm2) and mini‐module (11.52 cm2) obtained the efficiencies of 22.03 % and 18.57 %, which are among the highest efficiencies for MAPbI3 based ones.
A DNA Origami‐Based Gene Editing System for Efficient Gene Therapy in Vivo
Authors: Tang Wantao , Tong Ting , Wang Hong , Lu Xuehe , Yang Changping , Wu Yushuai , Wang Yuang , Liu Jianbing , Ding Baoquan 
Published: October 2023

AbstractDNA nanostructures have played an important role in the development of novel drug delivery systems. Herein, we report a DNA origami‐based CRISPR/Cas9 gene editing system for efficient gene therapy in vivo. In our design, a PAM‐rich region precisely organized on the surface of DNA origami can easily recruit and load sgRNA/Cas9 complex by PAM‐guided assembly and pre‐designed DNA/RNA hybridization. After loading the sgRNA/Cas9 complex, the DNA origami can be further rolled up by the locking strands with a disulfide bond. With the incorporation of DNA aptamer and influenza hemagglutinin (HA) peptide, the cargo‐loaded DNA origami can realize the targeted delivery and effective endosomal escape. After reduction by GSH, the opened DNA origami can release the sgRNA/Cas9 complex by RNase H cleavage to achieve a pronounced gene editing of a tumor‐associated gene for gene therapy in vivo. This rationally developed DNA origami‐based gene editing system presents a new avenue for the development of gene therapy.
Electrochemical Azidocyanation of Alkenes
Authors: Zheng Yun‐Tao , Xu Hai‐Chao 
Published: October 2023

AbstractThe difunctionalization of alkenes—a process that installs two functional groups in a single operation and transforms chemical feedstocks into value‐added products—is one of the most appealing synthetic methods in contemporary chemistry. However, the introduction of two distinct functional groups via two readily accessible nucleophiles remains a formidable challenge. Existing intermolecular alkene azidocyanation methods, which primarily focus on aryl alkenes and rely on stoichiometric chemical oxidants. We report herein an unprecedented electrochemical strategy for alkene azidocyanation that is compatible with both alkyl and aryl alkenes. This is achieved by harnessing the finely‐tuned anodic electron transfer and the strategic selection of copper/ligand complexes. The reactions of aryl alkenes were rendered enantioselective by employing a chiral ligand. Crucially, the mild conditions and well‐regulated electrochemical process assure exceptional tolerance for various functional groups and substrate compatibility with both terminal and internal alkyl alkenes.
Naphthyl Substituted Impurities Induce Efficient Room Temperature Phosphorescence
Authors: Qiao Weiguo , Yao Ming , Xu Jingwen , Peng Haiyan , Xia Jianlong , Xie Xiaolin , Li Zhong'an 
Published: October 2023

AbstractAccidentally, it was found that triphenylamine (TPA) from commercial sources shows ultralong yellow‐green room temperature phosphorescence (RTP) like commercial carbazole, which however disappears for lab‐synthesized TPA with high purity. Herein, we for the first time identify the impurity types that cause RTP of commercial TPA, which are two N, N‐diphenyl‐naphthylamine isomers. Due to similar molecular polarity and very trace amount (≈0.8 ‰, molar ratio), these naphthyl substituted impurities can be easily overlooked. We further show that even at an extremely low amount (1000000 : 1, mass ratio) of impurities, RTP emission is still generated, attributed to the triplet‐to‐triplet energy transfer mechanism. Notably, this doping strategy is also applicable to the triphenylphosphine and benzophenone host systems, of which strong RTP emission can be activated by simply doping the corresponding naphthyl substituted analogues into them. This work therefore provides a general and efficient host/guest strategy toward high performance and diverse organic RTP materials.
Prognostic power assessment of clinical parameters to predict neoadjuvant response therapy in HER2‐positive breast cancer patients: A machine learning approach
Authors: Fanizzi Annarita , Latorre Agnese , Bavaro Domenica Antonia , Bove Samantha , Comes Maria Colomba , Di Benedetto Erika Francesca , Fadda Federico , La Forgia Daniele , Giotta Francesco , Palmiotti Gennaro , Petruzzellis Nicole , Rinaldi Lucia , Rizzo Alessandro , Lorusso Vito , Massafra Raffaella 
Published: October 2023

AbstractBackgroundAbout 15%–20% of breast cancer (BC) cases is classified as Human Epidermal growth factor Receptor type 2 (HER2) positive. The Neoadjuvant chemotherapy (NAC) was initially introduced for locally advanced and inflammatory BC patients to allow a less extensive surgical resection, whereas now it represents the current standard for early‐stage and operable BC. However, only 20%–40% of patients achieve pathologic complete response (pCR). According to the results of practice‐changing clinical trials, the addition of trastuzumab to NAC brings improvements to pCR, and recently, the use of pertuzumab plus trastuzumab has registered further statistically significant and clinically meaningful improvements in terms of pCR. The goal of our work is to propose a machine learning model to predict the pCR to NAC in HER2‐positive patients based on a subset of clinical features.MethodFirst, we evaluated the significant association of clinical features with pCR on the retrospectively collected data referred to 67 patients afferent to Istituto Tumori “Giovanni Paolo II.” Then, we performed a feature selection procedure to identify a subset of features to be used for training a machine learning‐based classification algorithm. As a result, pCR to NAC was associated with ER status, Pgr status, and HER2 score.ResultsThe machine learning model trained on a subgroup of essential features reached an AUC of 73.27% (72.44%–73.66%) and an accuracy of 71.67% (71.64%–73.13%). According to our results, the clinical features alone are not enough to define a support system useful for clinical pathway.ConclusionOur results seem worthy of further investigation in large validation studies and this work could be the basis of future study that will also involve radiomics analysis of biomedical images.
Are they humans or are they robots? The effect of virtual influencer disclosure on brand trust
Authors: Muniz Fernanda , Stewart Kristin , Magalhães Lívia 
Published: October 2023

AbstractThe increased prevalence of social media has led brands to utilize influencers for product endorsements and influence consumer perceptions. Among these influencers, virtual influencers (VIs)—that is, computer‐generated influencers with a social media presence–have gained significant attention due to their substantial online following and greater engagement compared to human influencers. However, a research gap exists in understanding the impact of VIs on consumer response toward brands, particularly when consumers perceive VIs as human. To address this gap, this study draws upon anthropomorphism and expectation disconfirmation theories to investigate the effect of disclosing a VI's nonhuman nature on consumer trust in the brand. Additionally, the influence of culture on consumers' perceptions of VIs is explored. The study employs three within‐subject experimental designs, with two studies conducted in the United States (n = 75 and n = 101) and one in Brazil (n = 83). The findings reveal that the disclosure of a VI's nonhuman nature negatively affects the perceived anthropomorphism of the influencer, subsequently diminishing the credibility of the VI and impacting brand trust. This study contributes significantly to the consumer behavior literature by examining the unintended consequences of disclosing VIs' nonhuman nature on consumer perceptions. Furthermore, it highlights the potential role of culture, as the direct influence of disclosure on anthropomorphism is observed, but the indirect influence on brand trust is not supported among Brazilian consumers. Consequently, brands must reconsider the design of their own VIs or those they collaborate with, striving for a design that mitigates consumer suspicion avoiding the negative effects of disclosure.
Are couple‐based psychological interventions beneficial for the mental health of prostate cancer patients and their spouses? A systematic review and meta‐analysis
Authors: Shen Bin , Sun Jianhong , Yu Zhenliang , Xu Gang , Zhou Yaoying 
Published: October 2023

AbstractAs the survival rates for prostate cancer (PCa) have improved, there has been an increasing focus on the mental health of couples affected by this condition. There have been several couple‐based psychological interventions, and yet the impact of this treatment modality on the mental health of PCa patients and their spouses is unclear. Consequently, a systematic search was conducted in PubMed, Embase, Cochrane, LILACS, and Web of Science up to March 2023 for randomized controlled trials (RCTs) addressing the impacts of couple‐based psychological interventions on both PCa patients and their spouses. Besides, the Cochrane Risk of Bias Assessment Tool was employed to evaluate the methodological quality and potential bias of the included studies. Moreover, statistical analysis and meta‐analysis were performed utilizing Revman 5.4, and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) was employed to assess the certainty of evidence. A total of nine RCTs were included, utilizing anxiety, depression, distress, communication, and self‐efficacy as outcome indicators to assess mental health. Results demonstrated that couple‐based psychological interventions increased spouses' self‐efficacy (WMD, 0.21; 95% CI: −0.00 ~ 0.42; P = 0.05) and communication (SMD, 0.34; 95% CI: 0.09 ~ 0.59; P = 0.009), while reducing their distress (SMD, −0.21; 95% CI: −0.40 ~ −0.02; P = 0.03). Nonetheless, there is a need for additional research on the effect of couple‐based psychological interventions on the mental health of PCa‐affected couples given the limited evidence supporting this conclusion.
Two‐ and three‐piece implants to boost data generation in preclinical in vivo research—A short technical report
Authors: Stavropoulos Andreas , Bellon Benjamin , Pipenger Benjamin , Andersen Ole Z. 
Published: October 2023

AbstractThe purpose of this technical report is to present two novel experimental implant designs to boost data generation in preclinical in vivo research. Specifically, the report describes the rationale and the components of (1) a two‐piece experimental implant suitable for a small animal platform (e.g., the rabbit femur/tibial epiphysis model), consisting of a threaded apical‐ and a coronal cylindrical piece, which is intended for collecting two types of biomechanical data, and (2) a three‐piece experimental implant suitable for a large animal platform (e.g., the mini‐pig mandible model), consisting of an apical “wound chamber”, which allows the collection of histological/histomorphometrical data, and a middle threaded and coronal cylindrical piece, which also allow the collection of two types of biomechanical data. The increased volume of information generated from a single experiment in a small animal platform, using the proposed two‐piece implant design, may assist in a more qualified decision‐making process, on whether it is relevant to proceed to further assessment using a large animal platform. Furthermore, the increased volume of information generated in a single animal experiment either in a small or large animal platform, using the proposed two‐ and three‐piece implants, respectively, likely decreases the number of animals otherwise needed for collecting the same information with standard one‐piece implants and, thus, contributes to the reduction/refinement elements of the 3R principle.