bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profil Sumber Daya Perikanan di Kecamatan 2x11 Enam Lingkung Kabupaten Padang Pariaman Provinsi Sumatera Barat
Authors: Maharani Silvia , Rina . , Yuniarti Tatty 
Published: December 2023

Kecamatan 2x11 Enam Lingkung memiliki potensi perikanan pada kegiatan usaha budidaya ikan air tawar pada bidang pembenihan gurami dan nila juga kegiatan pembesaran gurami dan nila. kelompok perikanan berjumlah sebanyak 20 pondakan di bina oleh satu penyuluh perikanan bantu di kecamatan 2x11 Enam Lingkung. Tujuan dari penelitian adalah melakukan identifikasi potensi wilayah guna mengetahui keadaan sumber daya alam, sumber daya manusia, sumber daya penunjang, sistem produksi pada wilayah perikanan yang potensial untuk di kembangkan. Penelitian ini dilaksanakan pada tanggal 02 November – 16 Desember 2022. Penentuan sampel secara Purpiosive Sampling dari populasi kelompok yang terdata pada Dinas Perikanan Kabupaten Padang Pariaman. Pengambilan data melalui observasi dan wawancara menggunakan kuesioner. Data analisis menggunakan analisis deskriptif kualitatif dan kuatitaif. Hasil identifikasi menunjukan bahwa kegiatan budidaya di Kecamatan 2x11 Enam Lingkung di dominasi oleh pembenihan dan pendederan komuditas ikan gurami dan lele sedangkan pada kegiatan pembesaran komuditas ikan gurami dan nila. Teknologi yang digunakan masih bersifat tradisional dan semi tradisional. Produksi pembenihan gurami rata-rata SR 67% gurami dan 71% lele sedangkan pembesaran ikan gurami memiliki FCR 2 dan ikan nila memiliki FCR 1,7. Alur pemasaran yang digunakan pembudidaya yaitu alur pemasaran semi langsung dan tidak langsung. Kegiatan penyuluhan yang dilakukan secara anjangsana.
Association of carotid wall shear stress measured by vector flow mapping technique with ba-PWV: a pilot study
Authors: Cheng Yi , Chen Jie , Zhao Qing , Zhang Jinghan , Gao Junyi 
Published: December 2023

ObjectiveArterial stiffness is an important tissue biomarker of the progression of atherosclerotic diseases. Brachial-ankle pulse wave velocity (ba-PWV) is a gold standard of arterial stiffness measurement widely used in Asia. Changes in vascular wall shear stress (WSS) lead to artery wall remodeling, which could give rise to an increase in arterial stiffness. The study aimed to explore the association between ba-PWV and common carotid artery (CCA) WSS measured by a newly invented vascular vector flow mapping (VFM) technique.MethodsWe included 94 subjects free of apparent cardiovascular disease (CVD) and divided them into a subclinical atherosclerosis (SA) group (N = 47) and non subclinical atherosclerosis (NSA) group (N = 47). CCA WSS was measured using the VFM technique. Bivariate correlations between CCA WSS and other factors were assessed with Pearson's, Spearman's, or Kendall's coefficient of correlation, as appropriate. Partial correlation analysis was conducted to examine the influence of age and sex. Multiple linear stepwise regression was used for the analysis of independent determinants of CCA WSS. Receiver operating characteristic (ROC) analysis was performed to find the association between CCA WSS and 10-year CVD risk.ResultsThe overall subjects had a mean age of 47.9 ± 11.2 years, and males accounted for 52.1%. Average systolic CCA WSS was significantly correlated with ba-PWV (r = −0.618, p &lt; 0.001) in the SA group. Multiple linear stepwise regression analysis confirmed that ba-PWV was an independent determinant of average systolic CCA WSS (β = −0.361, p = 0.003). The area under the curve (AUC) of average systolic CCA WSS for 10-year CVD risk ≥10% was 0.848 (p &lt; 0.001) in the SA group.ConclusionsAverage systolic CCA WSS was significantly correlated with ba-PWV and was associated with 10-year CVD risk ≥10% in the SA group. Therefore, CCA WSS measured by the VFM technique could be used for monitoring and screening subjects with potential CVD risks.
Single-cell RNA sequencing analysis of lung cells in COVID-19 patients with diabetes, hypertension, and comorbid diabetes-hypertension
Authors: Zhang Xin , Deng Xiaoqian , Zhang Liangliang , Wang Pengbo , Tong Xia , Mo Yan , Zhang Yuansheng , Zhang Yan , Mo Chunheng , Zhang Lanlan 
Published: December 2023

BackgroundThere is growing evidence that the lung is a target organ for injury in diabetes and hypertension. There are no studies on the status of the lungs, especially cellular subpopulations, and related functions in patients with diabetes, hypertension, and hypertension-diabetes after combined SARS-CoV-2 infection.MethodUsing single-cell meta-analysis in combination with bulk-RNA analysis, we identified three drug targets and potential receptors for SARS-CoV-2 infection in lung tissues from patients with diabetes, hypertension, and hypertension-diabetes, referred to as “co-morbid” patients. Using single-cell meta-analysis analysis in combination with bulk-RNA, we identified drug targets and potential receptors for SARS-CoV-2 infection in the three co-morbidities.ResultsThe single-cell meta-analysis of lung samples from SARS-CoV-2-infected individuals with diabetes, hypertension, and hypertension-diabetes comorbidity revealed an upregulation of fibroblast subpopulations in these disease conditions associated with a predictive decrease in lung function. To further investigate the response of fibroblasts to therapeutic targets in hypertension and diabetes, we analyzed 35 upregulated targets in both diabetes and hypertension. Interestingly, among these targets, five specific genes were upregulated in fibroblasts, suggesting their potential association with enhanced activation of endothelial cells. Furthermore, our investigation into the underlying mechanisms driving fibroblast upregulation indicated that KREMEN1, rather than ACE2, could be the receptor responsible for fibroblast activation. This finding adds novel insights into the molecular processes involved in fibroblast modulation in the context of SARS-CoV-2 infection within these comorbid conditions. Lastly, we compared the efficacy of Pirfenidone and Nintedanib as therapeutic interventions targeting fibroblasts prone to pulmonary fibrosis. Our findings suggest that Nintedanib may be a more suitable treatment option for COVID-19 patients with diabetes and hypertension who exhibit fibrotic lung lesions.ConclusionIn the context of SARS-CoV-2 infections, diabetes, hypertension, and their coexistence predominantly lead to myofibroblast proliferation. This phenomenon could be attributed to the upregulation of activated endothelial cells. Moreover, it is noteworthy that therapeutic interventions targeting hypertension-diabetes demonstrate superior efficacy. Regarding treating fibrotic lung conditions, Nintedanib is a more compelling therapeutic option.
High incidence of occult familial SDHD cases amongst Czech patients with head and neck paragangliomas
Authors: Guha Anasuya , Vicha Ales , Zelinka Tomas , Kana Martin , Musil Zdenek , Pacak Karel , Betka Jan , Chovanec Martin , Plzak Jan , Boucek Jan 
Published: December 2023

IntroductionHead and neck paragangliomas (HNPGLs) are rare neuroendocrine tumors, which are mostly benign in nature. Amongst all genes, Succinate Dehydrogenase Subunit D (SDHD) is the most commonly mutated in familial HNPGLs. In about 30% of HNPGLs, germline mutations in SDHD can also occur in the absence of positive family history, thus giving rise to “occult familial” cases. Our aim was to evaluate the pattern of SDHD germline mutations in Czech patients with HNPGLs.Materials and methodsWe analyzed a total of 105 patients with HNPGLs from the Otorhinolaryngology departments of 2 tertiary centers between 2006 – 2021. All underwent complex diagnostic work-up and were also consented for genetic analysis.ResultsEighty patients aged 13-76 years were included; around 60% with multiple PGLs were males. Carotid body tumor was the most frequently diagnosed tumor. Germline SDHD mutation was found in only 12% of the Czech patients; approximately 78% of those harboring the mutation had negative family history. The mutation traits had higher affiliation for multiple tumors with nearly 70% patients of ≤ 40 years of age.ConclusionAn SDHD mutation variant was shared amongst unrelated patients but no founder-effect was established. Our findings confirmed that the pattern of SDHD mutation distribution amongst HNPGLs in Czech Republic differs from most studies worldwide.
Early warning analysis of mountain flood disaster based on Copula function risk combination
Authors: Chai Qihui , Wan Fang , Zhang Fei , Wu Feng , Han Wenhao , Ding Zelin , Yang Shifeng 
Published: December 2023

Mountain torrent disaster prevention is the focus of flood control and disaster reduction in China. Critical rainfall is an important indicator to determine the success or failure of mountain torrent disaster early warning. In this paper, the M-Copula function is introduced, the multi-dimensional joint distribution of critical rainfall is constructed, and the joint distribution of rainfall and peak rainfall intensity is analyzed. Taking A village in Xinxian County as an example. The critical rainfall of the combined probability is calculated, and the critical rainfall of the flash flood disaster water level, the pre-shift warning and the sharp-shift warning is warned and analyzed. The results show that the flood peak modulus calculated by Yishangfan group is 8.89, which has certain rules for the flood peak modulus of rivers in hilly areas. The larger the basin area is, the smaller the flood peak modulus is, the smaller the area is, and the larger the flood peak modulus is. The calculation result of the design flow of 533 m3/s is reasonable. It is reasonable and reliable to select the M-Copula function as the connection function to fit the joint distribution of rainfall and peak rainfall intensity, which can provide theoretical support for flash flood disaster warning in other regions.
Prior viral infection primes cross-reactive CD8+ T cells that respond to mouse heart allografts
Authors: Khorki M. Eyad , Shi Tiffany , Cianciolo Eileen E. , Burg Ashley R. , Chukwuma P. Chukwunalu , Picarsic Jennifer L. , Morrice Mary K. , Woodle E. Steve , Maltzman Jonathan S. , Ferguson Autumn , Katz Jonathan D. , Baker Brian M. , Hildeman David A. 
Published: December 2023

IntroductionSignificant evidence suggests a connection between transplant rejection and the presence of high levels of pre-existing memory T cells. Viral infection can elicit viral-specific memory T cells that cross-react with allo-MHC capable of driving allograft rejection in mice. Despite these advances, and despite their critical role in transplant rejection, a systematic study of allo-reactive memory T cells, their specificities, and the role of cross-reactivity with viral antigens has not been performed.MethodsHere, we established a model to identify, isolate, and characterize cross-reactive T cells using Nur77 reporter mice (C57BL/6 background), which transiently express GFP exclusively upon TCR engagement. We infected Nur77 mice with lymphocytic choriomeningitis virus (LCMV-Armstrong) to generate a robust memory compartment, where quiescent LCMV-specific memory CD8+ T cells could be readily tracked with MHC tetramer staining. Then, we transplanted LCMV immune mice with allogeneic hearts and monitored expression of GFP within MHC-tetramer defined viral-specific T cells as an indicator of their ability to cross-react with alloantigens.ResultsStrikingly, prior LCMV infection significantly increased the kinetics and magnitude of rejection as well as CD8+ T cell recruitment into allogeneic, but not syngeneic, transplanted hearts, relative to non-infected controls. Interestingly, as early as day 1 after allogeneic heart transplant an average of ~8% of MHC-tetramer+ CD8+ T cells expressed GFP, in contrast to syngeneic heart transplants, where the frequency of viral-specific CD8+ T cells that were GFP+ was &lt;1%. These data show that a significant percentage of viral-specific memory CD8+ T cells expressed T cell receptors that also recognized alloantigens in vivo. Notably, the frequency of cross-reactive CD8+ T cells differed depending upon the viral epitope. Further, TCR sequences derived from cross-reactive T cells harbored distinctive motifs that may provide insight into cross-reactivity and allo-specificity.DiscussionIn sum, we have established a mouse model to track viral-specific, allo-specific, and cross-reactive T cells; revealing that prior infection elicits substantial numbers of viral-specific T cells that cross-react to alloantigen, respond very early after transplant, and may promote rapid rejection.
Cellular and transcriptome signatures unveiled by single-cell RNA-Seq following ex vivo infection of murine splenocytes with Borrelia burgdorferi
Authors: Kumaresan Venkatesh , Ingle Taylor MacMackin , Kilgore Nathan , Zhang Guoquan , Hermann Brian P. , Seshu Janakiram 
Published: December 2023

IntroductionLyme disease, the most common tick-borne infectious disease in the US, is caused by a spirochetal pathogen Borrelia burgdorferi (Bb). Distinct host responses are observed in susceptible and resistant strains of inbred of mice following infection with Bb reflecting a subset of inflammatory responses observed in human Lyme disease. The advent of post-genomic methodologies and genomic data sets enables dissecting the host responses to advance therapeutic options for limiting the pathogen transmission and/or treatment of Lyme disease.MethodsIn this study, we used single-cell RNA-Seq analysis in conjunction with mouse genomics exploiting GFP-expressing Bb to sort GFP+ splenocytes and GFP− bystander cells to uncover novel molecular and cellular signatures that contribute to early stages of immune responses against Bb.ResultsThese data decoded the heterogeneity of splenic neutrophils, macrophages, NK cells, B cells, and T cells in C3H/HeN mice in response to Bb infection. Increased mRNA abundance of apoptosis-related genes was observed in neutrophils and macrophages clustered from GFP+ splenocytes. Moreover, complement-mediated phagocytosis-related genes such as C1q and Ficolin were elevated in an inflammatory macrophage subset, suggesting upregulation of these genes during the interaction of macrophages with Bb-infected neutrophils. In addition, the role of DUSP1 in regulating the expression of Casp3 and pro-inflammatory cytokines Cxcl1, Cxcl2, Il1b, and Ccl5 in Bb-infected neutrophils were identified.DiscussionThese findings serve as a growing catalog of cell phenotypes/biomarkers among murine splenocytes that can be exploited for limiting spirochetal burden to limit the transmission of the agent of Lyme disease to humans via reservoir hosts.
Variability in the effects of bilingualism on task switching of cognitively healthy and cognitively impaired older bilinguals
Authors: Chen Hui-Ching , Yow W. Quin 
Published: December 2023

IntroductionThe impact of bilingualism on executive function has been extensively discussed, but inconsistent evidence has been reported. These discrepancies may stem from the complexities of being bilingual and the various ways of measuring bilingual experiences. This study aims to clarify the debate by providing a systematic critique and analysis on how different measurements of bilingualism can lead to different results within the same group of bilinguals.MethodsWe tested 48 cognitively healthy (CH) and 43 cognitively impaired (CI) older adults (Mage = 73.25 and 79.72 years, respectively) using the color-shape switching task. We assessed bilingualism using six different methods based on dominant language usage: five categorical computations and one continuous measurement.Results and discussionThe results varied depending on the method of measuring bilingualism and the participant group. For CH older adults, a significant effect of bilingualism on cognition performance was observed only when using the categorical variable based on a cutoff of 70% dominant language usage, but not with other categorical computations or the continuous approach. For CI older adults, no effect of bilingualism was found, regardless of the type of measurement used. In summary, our results demonstrated that different measurements of language use can yield different results within the same group of bilinguals using a single task. Our study yielded important implications for bilingual research: the findings challenge the current methodologies used to describe bilingual experiences and call for care and consideration of context and the complexity when examining the effects of bilingual experience on executive functions.
A comparative systematic review and meta-analysis on the diagnostic accuracy of non-invasive tests for Helicobacter pylori detection in elderly patients
Authors: Omar Mahmud , Abu-Salah Razi , Agbareia Reem , Sharif Yusra , Levin Roni , Lahat Adi , Sharif Kassem 
Published: December 2023

BackgroundHelicobacter pylori (H. pylori) infection, a type I carcinogen, affects approximately 50% of the global population, correlating with various gastric pathologies. Notably, diagnostic sensitivities of non-invasive methods, such as the stool antigen test (HpSA), Serology, and Urea Breath Test (UBT), have been suggested to be less effective in older age groups. This study systematically reviews and meta-analyzes the diagnostic accuracy of these tests within the elderly population.MethodsA comprehensive literature search was performed across multiple databases, including PubMed, Medline, and Web of Science, up to July 2023. Data were pooled and analyzed using random-effects models. Sensitivity, specificity, and Diagnostic Odds Ratios (DOR) were computed for the tests. Heterogeneity and risk of bias were assessed.ResultsEight studies involving diverse geographic locations and totaling between 46 and 1,441 participants per study were included. The pooled sensitivity and specificity for HpSA were 72.5 and 94.7%, for Serology 83.7 and 73.3%, and for UBT 96.4 and 88.3%, respectively. DOR for UBT, HpSA, and Serology were 94.5, 47.9, and 14.2, respectively. High levels of heterogeneity were observed across the studies.ConclusionUBT and HpSA proved effective for diagnosing H. pylori in those over 60, while serology showed lower specificity. Despite methodological variations in available studies, these non-invasive tests offer reliable alternatives, especially for older patients who recently undergone endoscopy or without an indication for it, warranting consideration by healthcare practitioners.
Physiotherapy case reports on three people with progressive supranuclear palsy
Authors: Mateus Mariana , Castro Caldas Alexandre 
Published: December 2023

IntroductionProgressive supranuclear palsy (PSP) is a neurodegenerative brain disease that affects patient’s functionality and quality of life. Physiotherapy should be recommended at the time of diagnosis to slow the progression of disability and enhance the quality of life of these patients.Clinical presentationHere, we describe three cases of patients with PSP, outlining their motor and non-motor symptoms and examining their clinical progression with physiotherapy intervention. During the initial intervention years, a reduction in the number of falls was achieved, along with improvements in gait and balance.ConclusionExercise and physiotherapy appear to be beneficial for patients with PSP by enhancing their functionality and quality of life. Controlling or reducing the number of falls should be the primary goal of any intervention for patients with PSP.
Integrating fractional amplitude of low-frequency fluctuation and functional connectivity to investigate the mechanism and prognosis of severe traumatic brain injury
Authors: Li Biao , Li Wu-gen , Guo Yao , Wang Yang , Xu Lu-yang , Yang Yuan , Xu Shi-guo , Tan Zi-long , Mei Yu-ran , Wang Kai-yang 
Published: December 2023

ObjectiveFunctional magnetic resonance imaging (fMRI) has been used for evaluating residual brain function and predicting the prognosis of patients with severe traumatic brain injury (sTBI). This study aimed to integrate the fractional amplitude of low-frequency fluctuation (fALFF) and functional connectivity (FC) to investigate the mechanism and prognosis of patients with sTBI.MethodsSixty-five patients with sTBI were included and underwent fMRI scanning within 14 days after brain injury. The patient’s outcome was assessed using the Glasgow Outcome Scale—Extended (GOSE) at 6 months post-injury. Of the 63 patients who met fMRI data analysis standards, the prognosis of 18 patients was good (GOSE scores ≥ 5), and the prognosis of 45 patients was poor (GOSE scores ≤  4). First, we apply fALFF to identify residual brain functional differences in patients who present different prognoses and conjoined it in regions of interest (ROI)-based FC analysis to investigate the residual brain function of sTBI at the acute phase of sTBI. Then, the area under the curve (AUC) was used to evaluate the predictive ability of the brain regions with the difference of fALFF and FC values.ResultsPatients who present good outcomes at 6 months post-injury have increased fALFF values in the Brodmann area (7, 18, 31, 13, 39 40, 42, 19, 23) and decreased FC values in the Brodmann area (28, 34, 35, 36, 20, 28, 34, 35, 36, 38, 1, 2, 3, 4, 6, 13, 40, 41, 43, 44, 20, 28 35, 36, 38) at the acute phase of sTBI. The parameters of these alterations can be used for predicting the long-term outcomes of patients with sTBI, of which the fALFF increase in the temporal lobe, occipital lobe, precuneus, and middle temporal gyrus showed the highest predictive ability (AUC = 0.883).ConclusionWe provide a compensatory mechanism that several regions of the brain can be spontaneously activated at the acute phase of sTBI in those who present with a good prognosis in the 6-month follow-up, that is, a destructive mode that increases its fALFF in the local regions and weakens its FC to the whole brain. These findings provide a theoretical basis for developing early intervention targets for sTBI patients.
GIPC1 regulates MACC1-driven metastasis
Authors: Siegel Franziska , Schmidt Hannes , Juneja Manisha , Smith Janice , Herrmann Pia , Kobelt Dennis , Sharma Kamal , Fichtner Iduna , Walther Wolfgang , Dittmar Gunnar , Volkmer Rudolf , Rathjen Fritz G. , Schlag Peter M. , Stein Ulrike 
Published: December 2023

BackgroundIdentification of cancer metastasis-relevant molecular networks is desired to provide the basis for understanding and developing intervention strategies. Here we address the role of GIPC1 in the process of MACC1-driven metastasis. MACC1 is a prognostic indicator for patient metastasis formation and metastasis-free survival. MACC1 controls gene transcription, promotes motility, invasion and proliferation of colon cancer cells in vitro, and causes tumor growth and metastasis in mice.MethodsBy using yeast-two-hybrid assay, mass spectrometry, co-immunoprecipitation and peptide array we analyzed GIPC1 protein binding partners, by using the MACC1 gene promoter and chromatin immunoprecipitation and electrophoretic mobility shift assay we probed for GIPC1 as transcription factor. We employed GIPC1/MACC1-manipulated cell lines for in vitro and in vivo analyses, and we probed the GIPC1/MACC1 impact using human primary colorectal cancer (CRC) tissue.ResultsWe identified MACC1 and its paralogue SH3BP4 as protein binding partners of the protein GIPC1, and we also demonstrated the binding of GIPC1 as transcription factor to the MACC1 promoter (TSS to -60 bp). GIPC1 knockdown reduced endogenous, but not CMV promoter-driven MACC1 expression, and diminished MACC1-induced cell migration and invasion. GIPC1 suppression reduced tumor growth and metastasis in mice intrasplenically transplanted with MACC1-overexpressing CRC cells. In human primary CRC specimens, GIPC1 correlates with MACC1 expression and is of prognostic value for metastasis formation and metastasis-free survival. Combination of MACC1 and GIPC1 expression improved patient survival prognosis, whereas SH3BP4 expression did not show any prognostic value.ConclusionsWe identified an important, dual function of GIPC1 - as protein interaction partner and as transcription factor of MACC1 – for tumor progression and cancer metastasis.