bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling potential virulence determinants in
Vibrio
isolates from
Anadara tuberculosa
through whole genome analyses
Authors: Restrepo-Benavides Mariana , Lozano-Arce Daniela , Gonzalez-Garcia Laura Natalia , Báez-Aguirre Felipe , Ariza-Aranguren Gabriela , Faccini Daniel , Zambrano María Mercedes , Jiménez Pedro , Fernández-Bravo Ana , Restrepo Silvia , Guevara-Suarez Marcela 
Published: January 2024

ABSTRACT

The genus
Vibrio
includes pathogenic bacteria able to cause disease in humans and aquatic organisms, leading to disease outbreaks and significant economic losses in the fishery industry. Despite much work on
Vibrio
in several marine organisms, no specific studies have been conducted on
Anadara tuberculosa
. This is a commercially important bivalve species, known as “piangua hembra,” along Colombia’s Pacific coast. Therefore, this study aimed to identify and characterize the genomes of
Vibrio
isolates obtained from
A. tuberculosa
. Bacterial isolates were obtained from 14
A. tuberculosa
specimens collected from two locations along the Colombian Pacific coast, of which 17 strains were identified as
Vibrio
:
V. parahaemolyticus
(
n
= 12),
V. alginolyticus
(
n
= 3),
V. fluvialis
(
n
= 1), and
V. natriegens
(
n
= 1). Whole genome sequence of these isolates was done using Oxford Nanopore Technologies (ONT). The analysis revealed the presence of genes conferring resistance to β-lactams, tetracyclines, chloramphenicol, and macrolides, indicating potential resistance to these antimicrobial agents. Genes associated with virulence were also found, suggesting the potential pathogenicity of these
Vibrio
isolates, as well as genes for Type III Secretion Systems (T3SS) and Type VI Secretion Systems (T6SS), which play crucial roles in delivering virulence factors and in interbacterial competition. This study represents the first genomic analysis of bacteria within
A. tuberculosa
, shedding light on
Vibrio
genetic factors and contributing to a comprehensive understanding of the pathogenic potential of these
Vibrio
isolates.

IMPORTANCE

This study presents the first comprehensive report on the whole genome analysis of
Vibrio
isolates obtained from
Anadara tuberculosa
, a bivalve species of great significance for social and economic matters on the Pacific coast of Colombia. Research findings have significant implications for the field, as they provide crucial information on the genetic factors and possible pathogenicity of
Vibrio
isolates associated with
A. tuberculosa
. The identification of antimicrobial resistance genes and virulence factors within these isolates emphasizes the potential risks they pose to both human and animal health. Furthermore, the presence of genes associated with Type III and Type VI Secretion Systems suggests their critical role in virulence and interbacterial competition. Understanding the genetic factors that contribute to
Vibrio
bacterial virulence and survival strategies within their ecological niche is of utmost importance for the effective prevention and management of diseases in aquaculture practices.
Chronic Stress Dampens Lactobacillus Johnsonii–Mediated Tumor Suppression to Enhance Colorectal Cancer Progression
Authors: Cao Qiuhua , Zhao Mingrui , Su Yali , Liu Siliang , Lin Yanting , Da Huijuan , Yue Chongxiu , Liu Yiming , Jing Dongquan , Zhao Qixiang , Liu Ning , Du Juan , Zuo Zhanjie , Fu Yao , Chen Anqi , Birnbaumer Lutz , Yang Yong , Dai Beiying , Gao Xinghua 
Published: January 2024

Abstract

Colorectal cancer development and outcome are impacted by modifiable risk factors, including psychologic stress. The gut microbiota has also been shown to be linked to psychologic factors. Here, we found a marked deteriorative effect of chronic stress in multiple colorectal cancer models, including chemically induced (AOM/DSS), genetically engineered (APCmin/+), and xenograft tumor mouse models. RNA sequencing data from colon tissues revealed that expression of stemness-related genes was upregulated in the stressed colorectal cancer group by activated β-catenin signaling, which was further confirmed by results from ex vivo organoid analyses as well as in vitro and in vivo cell tumorigenicity assays. 16S rRNA sequencing of the gut microbiota showed that chronic stress disrupted gut microbes, and antibiotic treatment and fecal microbiota transplantation abolished the stimulatory effects of chronic stress on colorectal cancer progression. Stressed colorectal cancer mice displayed a significant decrease in Lactobacillus johnsonii (L. johnsonii) abundance, which was inversely correlated with tumor load. Moreover, protocatechuic acid (PCA) was identified as a beneficial metabolite produced by L. johnsonii based on metabolome sequencing and LC/MS-MS analysis. Replenishment of L. johnsonii or PCA blocked chronic stress-induced colorectal cancer progression by decreasing β-catenin expression. Furthermore, PCA activated the cGMP pathway, and the cGMP agonist sildenafil abolished the effects of chronic stress on colorectal cancer. Altogether, these data identify that stress impacts the gut microbiome to support colorectal cancer progression.

Significance:
Chronic stress stimulates cancer stemness by reducing the intestinal abundance of L. johnsonii and its metabolite PCA to enhance β-catenin signaling, forming a basis for potential strategies to circumvent stress-induced cancer aggressiveness.
See related commentary by McCollum and Shah, p. 645
Marital Status, Living Arrangement, and Survival among Individuals with Advanced Prostate Cancer in the International Registry for Men with Advanced Prostate Cancer
Authors: Chen Naiyu , McGrath Colleen B. , Ericsson Caroline I. , Vaselkiv Jane B. , Rencsok Emily M. , Stopsack Konrad H. , Guard Hannah E. , Autio Karen A. , Rathkopf Dana E. , Enting Deborah , Bitting Rhonda L. , Mateo Joaquin , Githiaka Charles W. , Chi Kim N. , Cheng Heather H. , Davis Ian D. , Anderson Simon G. , Badal Simone Ann Marie , Bjartell Anders , Russnes Kjell M. , Heath Elisabeth I. , Pomerantz Mark M. , Henegan John C. , Hyslop Terry , Esteban Emilio , Omlin Aurelius , McDermott Ray , Fay Andre P. , Popoola Ademola Alabi , Ragin Camille , Nowak Joel , Gerke Travis , Kantoff Philip W. , George Daniel J. , Penney Kathryn L. , Mucci Lorelei A. ,   for the IRONMAN Registry
Published: January 2024

Abstract

Background:
Studies have shown improved survival among individuals with cancer with higher levels of social support. Few studies have investigated social support and overall survival (OS) in individuals with advanced prostate cancer in an international cohort. We investigated the associations of marital status and living arrangements with OS among individuals with advanced prostate cancer in the International Registry for Men with Advanced Prostate Cancer (IRONMAN).

Methods:
IRONMAN is enrolling participants diagnosed with advanced prostate cancer (metastatic hormone-sensitive prostate cancer, mHSPC; castration-resistant prostate cancer, CRPC) from 16 countries. Participants in this analysis were recruited between July 2017 and January 2023. Adjusting for demographics and tumor characteristics, the associations were estimated using Cox regression and stratified by disease state (mHSPC, CRPC), age (&lt;70, ≥70 years), and continent of enrollment (North America, Europe, Other).

Results:
We included 2,119 participants with advanced prostate cancer, of whom 427 died during up to 5 years of follow-up (median 6 months). Two-thirds had mHSPC. Most were married/in a civil partnership (79%) and 6% were widowed. Very few married participants were living alone (1%), while most unmarried participants were living alone (70%). Married participants had better OS than unmarried participants [adjusted HR: 1.44; 95% confidence interval (CI): 1.02–2.02]. Widowed participants had the worst survival compared with married individuals (adjusted HR: 1.89; 95% CI: 1.22–2.94).

Conclusions:
Among those with advanced prostate cancer, unmarried and widowed participants had worse OS compared with married participants.

Impact:
This research highlighted the importance of social support in OS within this vulnerable population.
Black soldier fly larvae should be considered beyond their use as feedstuff
Authors: Tettamanti G. , Bruno D. 
Published: January 2024

Abstract
In recent years the entomological landscape has witnessed substantial progress in the promotion of insects for food and feed purposes. In particular, the black soldier fly (BSF) sector is experiencing an unprecedented expansion and is attracting growing attention from both researchers and entrepreneurs. Despite the well-established supply chain based on the valorisation of waste and by-products by BSF larvae for producing feedstuff, the BSF industry is considering alternative challenging substrates for rearing the larvae and exploring novel applications of bioactive molecules from BSF beyond animal feed. This editorial challenges the conventional boundaries of the insects for food and feed sector, and delves into the innovative use of BSF proteins, lipids, and chitin, pointing out diverse opportunities across the biomedical, biotechnological, agricultural, zootechnical, and environmental sectors that could further propel the utilisation of this insect.
Scutellaria wushanensis (Lamiaceae), a new species from Chongqing, China
Authors: ZHANG HUAN , ZHOU HOULIN , LV XINYUN , YANG YUBING , ZUO YOUWEI , XIA CHANGYING , YU JIE , DENG HONGPING 
Published: January 2024

Scutellaria wushanensis (Lamiaceae), found in the broad-leaf forest of Chongqing Wulipo National Nature Reserve, Chongqing, China, is described and illustrated as a new species after comparing it with related species. The new species shares a similar leaf shape and inflorescence morphology with Scutellaria lotienensis. However, the new species can be clearly distinguished from Scutellaria lotienensis by the bract shape, the pubescence inside the corolla, the hairless filaments, and the posterior corolla lobe shape and the purple-red patches.
Propofol Infusion Syndrome Following Endoscopic Tracheoplasty and Jet Ventilation: Case Report
Authors: Chen Bonnie , Tran Andrew , Alnijoumi Mohammed , Gilbert Mark R. 
Published: January 2024

Objective: We present a case of propofol infusion syndrome (PRIS) following jet ventilation. Method: Case report and review of literature. Results: A 70-year-old man required CO2 laser endoscopic tracheoplasty for tracheal and subglottic stenosis due to A-frame deformity. Postoperatively, the patient was reintubated for respiratory distress and propofol was resumed. Over the next two days the patient developed acute kidney injury, leukocytosis, acute primary respiratory acidosis with high anion gap metabolic acidosis, multiple end organ damage, elevated cardiac markers, and worsening lactic acidosis. The patient was recognized as having propofol infusion syndrome and propofol was immediately discontinued and replaced with dexmedetomidine. Unfortunately the patient progressed to multi-organ failure complicated by rhabdomyolysis and distributive intravascular coagulopathy. Conclusions: Propofol is often used as an anesthetic for jet ventilation during otolaryngologic airway surgery. Propofol related infusion syndrome is an uncommon but life-threatening peri-operative complication that should be considered in any patient with an unusual post-operative recovery characterized by metabolic acidosis, ECG changes, end organ damage, and elevated lactate.
Pilot Testing an Asynchronous Online Harm Reduction and Pharmacotherapy Stigma Reduction Training for Substance Use Treatment Professionals
Authors: Sulzer Sandra H. , Prevedel Suzanne , Barrett Tyson S. , Mekjian Margo , Vincent Mindy , Frabis Felicia , Meier Cris , Shiverdecker Claire Warnick , Voss Maren Wright , Cook Paula J. , Madden Erin Fanning 
Published: January 2024

Harm reduction and pharmacotherapy approaches to addressing substance use disorder are evidence-based practices for reducing adverse health outcomes. However, professional stigma toward these approaches impedes implementation. In this pilot study, professionals working in substance use treatment services received a 4-hour asynchronous online harm reduction and pharmacotherapy training. Pre- and post-training surveys used Likert-scale questions to assess attitudes and planned actions. Four of the 23 survey items demonstrated a significant change in pre- to post-attitudes and planned actions relating to harm reduction or pharmacotherapy. These items included less attitudinal stigma towards: methadone and buprenorphine ( p = .021), overdose prevention sites/“drug consumption facilities” ( p = .025), and naloxone distribution ( p = .017), as well as lower intent to primarily promote abstinence-based interventions ( p = .007). This study demonstrated that online asynchronous educational interventions show promise for reducing stigmatizing attitudes towards evidence-based practices among substance use treatment professionals.
Teaching Mathematics in Kindergarten: How Does Instruction Differ by Classroom Ability?
Authors: Gottfried Michael , Fletcher Tina , Comstock Meghan 
Published: January 2024

Background/Context: Kindergarten mathematics instruction is critical for students’ future academic success. The nature and quality of this instruction may vary depending on classroom characteristics. However, little empirical work has examined how mathematics instruction in kindergarten might differ based on classroom performance levels. Purpose, Objective, Research Question, or Focus of Study: This study focuses on whether kindergarten teachers’ mathematics instructional practices differ based on reported performance levels of students in the classroom. In particular, we focused on whether mathematics instructional time, as well as the extent to which teachers use traditional versus ambitious mathematics practices, differed based on teaching a higher proportion of children performing below grade level. Research Design: This study used a nationally representative dataset of approximately 2,900 kindergarten teachers from the Early Childhood Longitudinal Study, Kindergarten Class of 2010–11. With these data, the study utilized a model that compared teachers to each other within the same school (i.e., school fixed effects). Conclusions or Recommendations: This study found that mathematics instructional time did not differ based on the proportion of children reported to be below grade level in the classroom. However, how teachers taught mathematics differed: teachers who reported having a greater percentage of students performing below grade level used ambitious practices less frequently. These findings were not moderated by measures of teacher background or teachers’ reported expectations of their students.
Superior analgesic efficacy of preemptive low-dose ketorolac compared with parecoxib after total knee arthroplasty: A retrospective propensity score matching study
Authors: Iamthanaporn Khanin , Rojjanasirisawat Nuttawut , Yuenyongviwat Varah 
Published: January 2024

Background Total knee arthroplasty (TKA) is a routine orthopedic procedure often associated with significant postoperative pain. Efficient pain management is paramount for patient recovery, with nonsteroidal anti-inflammatory drugs (NSAIDs) being a common choice. Nevertheless, the specific NSAID and its dosing regimen can have varying impacts on outcomes. Methods In this retrospective cohort study spanning from January 2016 to December 2020, we analyzed patients who underwent TKA. These patients were divided into two groups: one receiving preemptive low-dose ketorolac (15 mg) followed by 15 mg every 6 h for 48 h, and the other receiving parecoxib (40 mg) every 12 h for the same duration. We assessed pain scores, opioid consumption, and monitored adverse events. Results Our findings reveal that ketorolac yielded superior results compared to parecoxib. Specifically, patients receiving ketorolac reported significantly lower Visual Numeric Rating Scale (VNRS) scores at 8- and 20-h post-surgery. This trend was further confirmed by linear mixed models ( p = .0084). Additionally, ketorolac was associated with reduced opioid consumption during the initial 24 h. Importantly, the rates of adverse events were comparable between the two groups. Conclusion The utilization of preemptive low-dose ketorolac demonstrates promising potential in bolstering pain control within the initial 24 h post-TKA, potentially reducing the need for opioids. However, further exploration is required to thoroughly assess its prolonged analgesic effects and safety across various surgical contexts. These investigations could provide invaluable insights for optimizing pain management protocols.
Integrating Frailty Assessment to Enhance Care in Cancer Patients with Borderline Eastern Cooperative Oncology Group Performance Status
Authors: Hsu Chih-Chung , Hung Yu-Shin , Yu Shao-Ming , Hsueh Shun-Wen , Chou Wen-Chi 
Published: January 2024

Background The (ECOG) performance status (PS) is commonly used to evaluate the functional ability of patients undergoing antitumor therapy. An ECOG PS of 2, indicating patients capable of self-care but restricted strenuous activity, can complicate treatment decisions owing to concerns regarding treatment-related toxicity. We investigated whether frailty assessment could help discriminate treatment tolerance and survival outcomes in patients with an ECOG PS of 2. Methods We prospectively included 45 consecutive patients, aged ≥65 years, with an ECOG PS of 2, and newly diagnosed solid cancer scheduled for chemotherapy. Frailty was assessed using an eight-indicator geriatric assessment. The primary outcome was overall survival (OS) based on frailty status; secondary outcomes included treatment tolerance and toxicity. Results The median patient age was 73 years (range 65-94), and 71% had stage IV disease. Predominant frailty-related deficits were functional decline (96%), malnutrition (78%), and polypharmacy (51%). The median OS was 12.6 months (95% confidence interval [CI]: 6.8-18.4). Patients with 4-6 deficits had significantly lower OS than those with 1-3 deficits (9.9 months vs. 20.0 months, adjusted hazard ratio 2.51, 95% CI: 1.16-5.44, P = .020). Frailty significantly correlated with reduced 12-week chemotherapy competence (52% vs. 85%, adjusted odds ratio [OR] .14, 95% CI: .03-.70, P = .016) and enhanced risk of unexpected hospitalization (60% vs. 20%, adjusted OR 6.80, 95% CI: 1.64-28.1, P = .008). Conclusion Our findings highlight the multifaceted nature of patients with an ECOG PS of 2 and emphasize the importance of frailty assessment for treatment outcomes.
Sub-Saharan women in engineering higher education: A literature-informed research tool
Authors: Hailu Meseret F. , Parra Gaete Ivet 
Published: January 2024

We present a review of higher education and education policy literature to understand better higher education institutions (HEIs) that serve sub-Saharan women in engineering. Our sub-Saharan women in engineering (SSAWE) literature-informed conceptual tool consists of five components to facilitate scholarly discussion about equity in higher education systems and facilitate interregional comparisons. The five components to study the educational experiences of women in engineering in this region include (1) a sociopolitical and historical national context for higher education policy, (2) critical discourse analysis of gender-based affirmative action policies, (3) enrollment and graduation data for female engineering students, (4) a longitudinal assessment of the employability of the engineering major, and (5) a qualitative campus climate survey of each country's flagship university. Our article introduces a conceptual investigation of the social, historical, and employment trends contextualizing engineering higher education in this region of Africa. We draw from existing literature about conceptual models predominantly used in higher education efforts toward undergraduate student success. When describing each SSAWE component, we include empirical and theoretical work that substantiates the need for each component and the benefits of the proposed methodology. More broadly speaking, this paper discusses how research employing the SSAWE framework could benefit key stakeholders who engage in policymaking. We suggest that sub-Saharan African universities should prioritize a regional comparative approach when designing support programs for underrepresented students in engineering. SSAWE will also generate more South–South comparisons that advance culturally situated analyses, contributing to higher education institutions’ advancementand the minoritized populations they serve.
Neuropsychiatric Behavioral Assessments in Mice After Acute and Long-Term Treatments of Low-Intensity Pulsed Ultrasound
Authors: Li Ye , Wu Yiqing , Luo Qi , Ye Xuanjie , Chen Jie , Su Yuanlin , Zhao Ke , Li Xinmin , Lin Jing , Tong Zhiqian , Wang Qi , Xu Dongwu 
Published: January 2024

Introduction: To evaluate whether both acute and chronic low-intensity pulsed ultrasound (LIPUS) affect brain functions of healthy male and female mice. Methods: Ultrasound (frequency: 1.5 MHz; pulse: 1.0 kHz; spatial average temporal average (SATA) intensity: 25 mW/cm2; and pulse duty cycle: 20%) was applied at mouse head in acute test for 20 minutes, and in chronic experiment for consecutive 10 days, respectively. Behaviors were then evaluated. Results: Both acute and chronic LIPUS at 25 mW/cm2 exposure did not affect the abilities of movements, mating, social interaction, and anxiety-like behaviors in the male and female mice. However, physical restraint caused struggle-like behaviors and short-time memory deficits in chronic LIPUS groups in the male mice. Conclusion: LIPUS at 25 mW/cm2 itself does not affect brain functions, while physical restraint for LIPUS therapy elicits struggle-like behaviors in the male mice. An unbound helmet targeted with ultrasound intensity at 25-50 mW/cm2 is proposed for clinical brain disease therapy.