bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cumulative Detection of Anal High-Grade Squamous Intraepithelial Lesions Over 2-Year Follow-up in Men Who Have Sex With Men Living With Human Immunodeficiency Virus in France
Authors: Combes Jean-Damien , Didelot Jean-Michel , Radenne Sylvie , Zaegel-Faucher Olivia , Lesage Anne-Carole , Siproudhis Laurent , Piroth Lionel , Marchand Lucie , Heard Isabelle , Hoyeau Nadia , Henno Sébastien , Darragh Teresa M , Alberts Catharina J , Clifford Gary M , Etienney Isabelle ,   ANRS-EP57-APACHES Study group, Arvieux Cédric , Canestri Ana , Clifford Gary , Combes Jean-Damien , Costes-Martineau Valérie , Del Grande Jean , Didelot Jean-Michel , Etienney Isabelle , Falguières Michael , Ferry Tristan , Fischer Hugues , Flejou Jean-Francois , Heard Isabelle , Henno Sébastien , Hoyau Nadia , Landon Marine , Laude Hélène , Le Breton Frédérique , Lesage Anne-Carole , Lion Annie , Maincent Gilles , Marchand Lucie , Andrianiaina Haingo Njatonirina , Patey Olivier , Sanchez Ventzislava Petrov , Pialoux Gilles , Pirel Marion , Piroth Lionel , Poizot-Martin Isabelle , Radenne Sylvie , Ressiot Emmanuelle , Reynes Jacques , Siproudhis Laurent , Tamalet Catherine , Tattevin Pierre , Zaegel-Faucher Olivia 
Published: November 2023

Abstract
We assessed cumulative detection and determinants of anal high-grade squamous intraepithelial lesions (HSILs) in men who have sex with men living with human immunodeficiency virus and who underwent 3 visits over 2 years, with cytology and high-resolution anoscopy, within the ANRS-EP57-APACHES study. The cumulative HSIL detection rate was 33% (134 of 410), of which 48% HSILs were detected at baseline. HSIL detection varied considerably by center (from 13% to 51%). The strongest HSIL determinants were baseline human papillomavirus 16 (adjusted odds ratio, 8.2; 95% confidence interval, 3.6–18.9) and p16/Ki67 (4.6 [2.3–9.1]). Repeated annual cytology and high-resolution anoscopy improved HSIL detection but did not fully compensate for between-center heterogeneity.
Physics-driven cycle network for seismic impedance inversion using conditional generative adversarial networks
Authors: Wang Yaojun , Zong Jingjing , Wang Liangji , Zou Bangli , Chen Ziteng , Luo Yang 
Published: November 2023

Abstract
Despite the extensive application of artificial neural networks in seismic inversion, their effectiveness is often hampered by the limited availability of labeled data. To address this challenge, we introduce a novel method for seismic impedance inversion. Our approach integrates a physics-driven cycle network with a conditional generative adversarial network (CGAN) and a convolutional model. Employing seismic data as the input, the CGAN capitalizes on inherent information to minimize non-uniqueness during inversion. Furthermore, the convolutional model, acting as a physics-informed operator, reverts the derived impedance data back to seismic form, enabling simultaneous training of neural networks with labeled and unlabeled data, fulfilling the seismic-to-seismic cycle. The proposed method is demonstrated to be effective on tests using both theoretical models and field data.
A multiplexed time-resolved fluorescence resonance energy transfer ultrahigh-throughput screening assay for targeting the SMAD4–SMAD3–DNA complex
Authors: Ouyang Wukun , Li Qianjin , Niu Qiankun , Qui Min , Fu Haian , Du Yuhong , Mo Xiulei 
Published: November 2023

Abstract
The transforming growth factor-beta (TGFβ) signaling pathway plays crucial roles in the establishment of an immunosuppressive tumor microenvironment, making anti-TGFβ agents a significant area of interest in cancer immunotherapy. However, the clinical translation of current anti-TGFβ agents that target upstream cytokines and receptors remains challenging. Therefore, the development of small-molecule inhibitors specifically targeting SMAD4, the downstream master regulator of the TGFβ pathway, would offer an alternative approach with significant therapeutic potential for anti-TGFβ signaling. In this study, we present the development of a cell lysate-based multiplexed time-resolved fluorescence resonance energy transfer (TR-FRET) assay in an ultrahigh-throughput screening (uHTS) 1536-well plate format. This assay enables simultaneous monitoring of the protein‒protein interaction between SMAD4 and SMAD3, as well as the protein‒DNA interaction between SMADs and their consensus DNA-binding motif. The multiplexed TR-FRET assay exhibits high sensitivity, allowing the dynamic analysis of the SMAD4–SMAD3–DNA complex at single-amino acid resolution. Moreover, the multiplexed uHTS assay demonstrates robustness for screening small-molecule inhibitors. Through a pilot screening of an FDA-approved bioactive compound library, we identified gambogic acid and gambogenic acid as potential hit compounds. These proof-of-concept findings underscore the utility of our optimized multiplexed TR-FRET platform for large-scale screening to discover small-molecule inhibitors that target the SMAD4–SMAD3–DNA complex as novel anti-TGFβ signaling agents.
Genotypic characterization of Streptococcus didelphis causative of fatal infection in white-eared opossums
Authors: Breyer Gabriela Merker , Rocha Jacques da Silva Maria Eduarda , Slaviero Mônica , Albuquerque de Almeida Bruno , Machado Sousa da Silva Emanoelly , de Queiroz Schmidt Victória Regina , Alievi Marcelo , Maboni Grazieli , Petinatti Pavarini Saulo , Maboni Siqueira Franciele 
Published: November 2023

Abstract
Streptococcus didelphis was once reported as related to severe infections in opossums. Thus, we present the first comprehensive whole-genome characterization of clinical S. didelphis strains isolated from white-eared opossums (Didelphis albiventris). Long-read whole-genome sequencing was performed using the MinION platform, which allowed the prediction of several genomic features. We observed that S. didelphis genomes harbor a cluster for streptolysin biosynthesis and a conserved genomic island with genes involved in transcriptional regulation (arlR) and transmembrane transport (bcrA). Antimicrobial resistance genes for several drug classes were found, including beta-lactam, which is the main antimicrobial class used in Streptococcus spp. infections; however, no phenotypical resistance was observed. In addition, we predicted the presence of 33 virulence factors in the analyzed genomes. High phylogenetic similarity was observed between clinical and reference strains, yet no clonality was suggested. We also proposed dnaN, gki, pros, and xpt as housekeeping candidates to be used in S. didelphis sequence typing. This is the first whole-genome characterization of S. didelphis, whose data provide important insights into its pathogenicity.
An empirical reionization history model inferred from the low-redshift Lyman continuum survey and the star-forming galaxies at z &gt; 8
Authors: Lin Yu-Heng , Scarlata Claudia , Williams Hayley , Chen Wenlei , Kelly Patrick , Langeroodi Danial , Hjorth Jens , Chisholm John , Koekemoer Anton M , Zitrin Adi , Diego Jose M 
Published: November 2023

ABSTRACT
We present a new analysis of the rest-frame ultraviolet (UV) and optical spectra of a sample of three z &gt; 8 galaxies discovered behind the gravitational lensing cluster RX J2129.4+0009. We combine these observations with z &gt; 7.5 galaxies from the literature, for which similar measurements are available. As already pointed out in other studies, the high [O iii]λ5007/[O ii]λ3727 ratios (O32) and steep UV continuum slopes (β) are consistent with the values observed for low-redshift Lyman continuum emitters, suggesting that such galaxies contribute to the ionizing budget of the intergalactic medium. We construct a logistic regression model to estimate the probability of a galaxy being a Lyman continuum emitter based on the measured MUV, β, and O32. Using this probability and the UV luminosity function, we construct an empirical model that estimates the contribution of high-redshift galaxies to reionization. The preferred scenario in our analysis shows that at z ∼ 8, the average escape fraction of the galaxy population [i.e. including both LyC emitters (LCEs) and non-emitters] varies with MUV, with intermediate UV luminosity (−19 &lt; MUV &lt; −16) galaxies having larger escape fraction. Galaxies with faint UV luminosity (−16 &lt; MUV &lt; −13.5) contribute most of the ionizing photons. The relative contribution of faint versus bright galaxies depends on redshift, with the intermediate UV galaxies becoming more important over time. UV bright galaxies, although more likely to be LCEs at a given log(O32) and β, contribute the least of the total ionizing photon budget.
Cosmic star-formation history and black hole accretion history inferred from the JWST mid-infrared source counts
Authors: Kim Seong Jin , Goto Tomotsugu , Ling Chih-Teng , Wu Cossas K-W , Hashimoto Tetsuya , Kilerci Ece , Ho Simon C-C , Uno Yuri , Wang Po-Ya , Lin Yu-Wei 
Published: November 2023

ABSTRACT
With the advent of the JWST, extragalactic source count studies were conducted down to sub-μJy in the mid-infrared (MIR), which is several tens of times fainter than what the previous-generation infrared (IR) telescopes achieved in the MIR. In this work, we aim to interpret the JWST source counts and constrain cosmic star-formation history (CSFH) and black hole accretion history (BHAH). We employ the backward evolution of local luminosity functions (LLFs) of galaxies to reproduce the observed source counts from sub-μJy to a few tens of mJy in the MIR bands of the JWST. The shapes of the LLFs at the MIR bands are determined using the model templates of the spectral energy distributions (SEDs) for five representative galaxy types (star-forming galaxies, starbursts, composite, AGN type 2 and 1). By simultaneously fitting our model to all the source counts in the six MIR bands, along with the previous results, we determine the best-fitting evolutions of MIR LFs for each of the five galaxy types, and subsequently estimate the CSFH and BHAH. Thanks to the JWST, our estimates are based on several tens of times fainter MIR sources, the existence of which was merely an extrapolation in previous studies.
Association of Neutrophil-to-Lymphocyte Ratio and Absolute Lymphocyte Count With Clinical Outcomes in Advanced Breast Cancer in the MONARCH 2 Trial
Authors: Tokunaga Eriko , Miyoshi Yasuo , Dozono Koji , Kawaguchi Tsutomu , Toi Masakazu 
Published: November 2023

Abstract

Background
Established prognostic factors for treatment response to cyclin-dependent kinases 4 and 6 inhibitors are currently lacking. We aimed to investigate the relationship of pretreatment neutrophil-to-lymphocyte ratio (NLR) and absolute lymphocyte count (ALC) to abemaciclib outcomes.

Patients and Methods
This was a post hoc analysis of data from MONARCH 2, a phase III study of abemaciclib or placebo plus fulvestrant in hormone-receptor positive (HR+), human epidermal growth factor receptor 2-negative (HER2−) advanced breast cancer that progressed on endocrine therapy. Patients were divided into high and low categories based on baseline NLR (cutoff: 2.5) and ALC (cutoff: 1.5 × 109/L). The association of baseline NLR and ALC with progression-free survival (PFS) and overall survival (OS) was explored using Cox models and Kaplan-Meier estimates. Tumor response and safety were also examined.

Results
NLR and ALC data were available for 645 patients (abemaciclib: N = 426; placebo: N = 219). Low-baseline NLR or high-baseline ALC was consistently associated with positive PFS and OS trends; low-baseline NLR subgroups also showed trends for better response. The abemaciclib treatment effect against placebo was observed regardless of baseline NLR or ALC. Univariate analyses showed baseline NLR and ALC were prognostic of PFS and OS. Baseline NLR remained significant in the multivariate model (P &lt; .0001). No unexpected differences in safety were observed by baseline NLR or ALC.

Conclusion
Baseline NLR was independently prognostic of PFS and OS. Low-baseline NLR was associated with numerically better efficacy outcomes, but the benefit of adding abemaciclib to fulvestrant was similar irrespective of baseline NLR status.
Managing an ageing workforce: workplace retention practices and early labour market exit
Authors: Qvist Jeevitha Yogachandiran 
Published: November 2023

Abstract
This study addresses a critical gap in our understanding of how the employer and workplace context influences the working career of older employees. Leveraging linked employer–employee data, this study examines the impact of workplace retention practices on an early labour market exit for employees aged fifty-five to sixty-four in Denmark. The findings reveal that, for those eligible for early retirement, work environment adaptation, re-employment of retired employees, and positive views of older employees’ productivity significantly contribute to avoiding early retirement. However, these workplace retention practices do not influence unemployment, emphasizing their effectiveness in postponing early retirement rather than mitigating job loss. Moreover, the analysis shows that employees with managerial roles and high skill levels benefit more from workplace retention practices in terms of avoiding both early retirement and unemployment. This finding highlights the employer’s key role in shaping inequalities in an ageing workforce by selectively providing opportunities to extend working lives.
Identification of ‘Candidatus Liberibacter asiaticus’, the Huanglongbing Bacterium, in Citrus from Colombia
Authors: Chaves-Sierra Catalina , Rodriguez-Cruz María Camila , Mejia-Alvarado Fernan Santiago , Ramírez-Higuera Carolina , Mejía-Eslava Antonio , Romero Hernán Mauricio 
Published: November 2023

‘Candidatus Liberibacter’ spp. are the most prevalent microorganisms in the citrus plant, associated with citrus huanglongbing, which are transmitted by psyllid vectors. In Colombia, the vector Diaphorina citri Kuwayama has been reported in different regions, but ‘Ca. Liberibacter asiaticus’ (CLas) has only been detected in insect vectors, not in citrus host plants. To identify the presence and quantify the pathogen in citrus tissues, we employed a combined strategy that involved three techniques based on polymerase chain reaction (PCR). First, we used endpoint PCR with specific primers for CLas (OI1/OI2c) to confirm the infection. Second, we used qPCR with specific primers CIT295a/CIT298 designed on 16S rDNA gene regions to quantify the pathogen load. Finally, we used droplet digital PCR (ddPCR) to determine the copy number of the pathogen in citrus tissues using the β-subunit of the ribonucleotide reductase gene (nrdB) that is specific to CLas. We identified the presence of CLas in citrus plants for the first time in Colombia and quantified its titer in the plant tissue. We employed ddPCR and qPCR to provide crucial information for the country’s disease management, control strategies, and general crop health. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Mustn1 ablation in skeletal muscle results in functional alterations
Authors: Kim Charles J. , Singh Chanpreet , Kaczmarek Marina , O'Donnell Madison , Lee Christine , DiMagno Kevin , Young Melody W. , Letsou William , Ramos Raddy L. , Granatosky Michael C. , Hadjiargyrou Michael 
Published: November 2023

AbstractMustn1, a gene expressed exclusively in the musculoskeletal system, was shown in previous in vitro studies to be a key regulator of myogenic differentiation and myofusion. Other studies also showed Mustn1 expression associated with skeletal muscle development and hypertrophy. However, its specific role in skeletal muscle function remains unclear. This study sought to investigate the effects of Mustn1 in a conditional knockout (KO) mouse model in Pax7 positive skeletal muscle satellite cells. Specifically, we investigated the potential effects of Mustn1 on myogenic gene expression, grip strength, alterations in gait, ex vivo investigations of isolated skeletal muscle isometric contractions, and potential changes in the composition of muscle fiber types. Results indicate that Mustn1 KO mice did not present any substantial phenotypic changes or significant variations in genes related to myogenic differentiation and fusion. However, an approximately 10% decrease in overall grip strength was observed in the 2‐month‐old KO mice in comparison to the control wild type (WT), but this decrease was not significant when normalized by weight. KO mice also generated approximately 8% higher vertical force than WT at 4 months in the hindlimb. Ex vivo experiments revealed decreases in about 20 to 50% in skeletal muscle contractions and about 10%–20% fatigue in soleus of both 2‐ and 4‐month‐old KO mice, respectively. Lastly, immunofluorescent analyses showed a persistent increase of Type IIb fibers up to 15‐fold in the KO mice while Type I fibers decreased about 20% and 30% at both 2 and 4 months, respectively. These findings suggest a potential adaptive or compensatory mechanism following Mustn1 loss, as well as hinting at an association between Mustn1 and muscle fiber typing. Collectively, Mustn1's complex roles in skeletal muscle physiology requires further research, particularly in terms of understanding the potential role of Mustn1 in muscle repair and regeneration, as well as with influence of exercise. Collectively, these will offer valuable insights into Mustn1's key biological functions and regulatory pathways.
The Optimal Age for Surgical Management of DDH Differs by Treatment Method
Authors: Martino Rachael , Carry Patrick , Adams Jordyn , Brandt Aaron , Sink Ernest , Selberg Courtney 
Published: November 2023

Background:
There is a lack of consensus on the optimal age for specific surgical interventions for developmental dysplasia of the hip. We compared radiographic and clinical outcomes among patients who were treated with closed reduction (CR), open reduction (OR), and open reduction with concomitant pelvic osteotomy (ORP) for the treatment of a dislocated hip. We sought to identify the optimal age at treatment within each of these groups.

Methods:
We retrospectively reviewed 256 hips (n=195 patients) who underwent CR (n=96), OR (n=116), or an ORP (n=44) as their index procedure at a single institution between January 1, 2004 and September 23, 2020. Radiographic outcomes included acetabular index, The International Hip Dysplasia Institute classification, and acetabular depth ratio. The incidence of further corrective surgery (FCS), defined as the need for an additional femoral and/or pelvic osteotomy before skeletal maturity, and the optimal age cutoffs for index surgery within each surgical group were determined.

Results:
After adjusting for age and sex, the incidence of FCS was 13.8% in the CR group, 29.2% in the OR group, and 9.2% in the ORP group. Earlier surgery was protective against FCS in the CR and OR groups. In contrast, patients in the ORP group who were older at index procedure were less likely to undergo FCS. Optimal age at surgery was 9.9 months (CR), 11.5 months (OR), and 21.4 months (ORP). Compared with older patients, younger patients were associated with a larger average decrease in the acetabular index and a larger average increase in acetabular width during the first 5 years post surgery.

Conclusions:
Age at index surgical procedure was correlated with both clinical and radiographic outcomes. Age at index procedure did impact the risk of subsequent FCS, particularly in the CR and ORP groups. Based on our analysis, CR should be considered before 9.9 months of age and OR considered before 11.5 months of age to minimize the risk of FCS during childhood. This work highlights the importance of considering age-related heterogeneity in developmental dysplasia of the hip treatment outcomes.

Level of Evidence:
Level III—retrospective comparative study.
Comparison of Bone Mineral Density in Children and Adolescents on CT Versus DEXA Scan
Authors: Nagata Kosei , Glassman Steven D. , Dimar John R. , Cabell Akaila , Brown Morgan , Daniels Christy , Schmidt Grant O. , Carreon Leah Y. 
Published: November 2023

Study Design.
Retrospective cross-sectional.

Objectives.
To determine associations between Hounsfield units (HU) within a region of interest on computed tomography (CT) scans and dual energy X-ray absorptiometry (DEXA) measurements in children and adolescents.

Summary of Background Data.
HU on CT scans as a proxy for bone mineral density (BMD) is widely used in adults. However, the utility of CT as a proxy for BMD have not been evaluated in children and adolescents.

Materials and Methods.
Patients younger than 18 years with a lumbar spine CT scan and a DEXA within 6 months of each other were identified. A region of interest was used to measure the HU at each lumbar vertebral body on midaxial cuts. Charts were reviewed for demographics, medical comorbidities, and DEXA reports. Correlation coefficients were calculated between the mean DEXA Z-score and the HU value. Patients were also stratified by Z-score (≥ −1.0, between −1.0 and −2.0, and ≤−2.0) and matched by age, sex and body mass index (BMI) to a cohort of healthy children and compared.

Results.
A moderate correlation between mean DEXA Z-score and mean HU on CT was found (r
2=0.42, P<0.001). After matching for age, sex and BMI, 21 patients in each group between the ages of 4 and 17 years were analyzed. The mean HU value of the control group was 231.69. When stratified by Z-score (≥ −1.0, between −1.0 and −2.0, and ≤−2.0), the mean HU values were 244.59, 216.50, and 176.54, respectively. Patients with a Z-score of ≤−2.0 had a significantly lower mean HU than matched controls.

Conclusions.
HU on lumbar CT in children and adolescents with DEXA Z-scores <−2.0, were lower when compared with healthy matched controls. This study suggests that HU on opportunistic CT scans of the spine may be used as a reasonable proxy for BMD in the pediatric population.
BODY-Q Normative Scores: Psychometric Validation of the BODY-Q in the General Population in Europe and North America
Authors: Dalaei Farima , de Vries Claire E.E. , Cano Stefan J. , Kaur Manraj N. , Poulsen Lotte , Pfob André , Mou Danny , Repo Jussi P. , Salzillo Rosa , Opyrchal Jakub , Sørensen Jens A. , Pusic Andrea L. , Klassen Anne F. 
Published: November 2023

Background:
BODY-Q is a rigorously developed patient-reported outcome measure designed to measure outcomes of weight loss and body contouring patients. To allow interpretation and comparison of BODY-Q scores across studies, normative BODY-Q values were generated from the general population. The aim of this study was to examine the psychometric properties of BODY-Q in the normative population.

Methods:
Data were collected using two crowdsourcing platforms (Prolific and Amazon Mechanical Turk) in 12 European and North American countries. Rasch measurement theory (RMT) was used to examine reliability and validity of BODY-Q scales.

Results:
RMT analysis supported the psychometric properties of BODY-Q in the normative sample with ordered thresholds in all items and nonsignificant chi-square values for 167 of 176 items. Reliability was high with person separation index of greater than or equal to 0.70 in 20 of 22 scales and Cronbach alpha values of greater than or equal to 0.90 in 17 of 22 scales. Mean scale scores measuring appearance, health-related quality of life, and eating-related concerns scales varied as predicted across subgroups with higher scores reported by participants who were more satisfied with their weight. Analysis to explore differential item functioning by sample (normative versus field-test) flagged some potential issues, but subsequent comparison of adjusted and unadjusted person estimates provided evidence that the scoring algorithm worked equivalently for the normative sample as in the field-test samples.

Conclusions:
The BODY-Q scales showed acceptable reliability and validity in the normative sample. The normative values can be used as reference in research and clinical practice in combination with local estimates for parallel analysis and comparison.