bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 spike receptor-binding domain is internalized and promotes protein ISGylation in human induced pluripotent stem cell-derived cardiomyocytes
Authors: Okuno Shota , Higo Shuichiro , Kondo Takumi , Shiba Mikio , Kameda Satoshi , Inoue Hiroyuki , Tabata Tomoka , Ogawa Shou , Morishita Yu , Sun Congcong , Ishino Saki , Honda Tomoyuki , Miyagawa Shigeru , Sakata Yasushi 
Published: December 2023

AbstractAlthough an increased risk of myocarditis has been observed after vaccination with mRNA encoding severe acute respiratory syndrome coronavirus 2 spike protein, its underlying mechanism has not been elucidated. This study investigated the direct effects of spike receptor-binding domain (S-RBD) on human cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs). Immunostaining experiments using ACE2 wild-type (WT) and knockout (KO) iPSC-CMs treated with purified S-RBD demonstrated that S-RBD was bound to ACE2 and internalized into the subcellular space in the iPSC-CMs, depending on ACE2. Immunostaining combined with live cell imaging using a recombinant S-RBD fused to the superfolder GFP (S-RBD-sfGFP) demonstrated that S-RBD was bound to the cell membrane, co-localized with RAB5A, and then delivered from the endosomes to the lysosomes in iPSC-CMs. Quantitative PCR array analysis followed by single cell RNA sequence analysis clarified that S-RBD-sfGFP treatment significantly upregulated the NF-kβ pathway-related gene (CXCL1) in the differentiated non-cardiomyocytes, while upregulated interferon (IFN)-responsive genes (IFI6, ISG15, and IFITM3) in the matured cardiomyocytes. S-RBD-sfGFP treatment promoted protein ISGylation, an ISG15-mediated post-translational modification in ACE2-WT-iPSC-CMs, which was suppressed in ACE2-KO-iPSC-CMs. Our experimental study demonstrates that S-RBD is internalized through the endolysosomal pathway, which upregulates IFN-responsive genes and promotes ISGylation in the iPSC-CMs.
Bioelectric device for effective biofilm inflammation management of dental implants
Authors: Lee Jihyun , Kim Young Wook 
Published: December 2023

AbstractDental implant inflammation is primarily caused by oral biofilms, which form within 8 h, particularly at 37 °C, thereby requiring diligent cleaning. Considering the complex management of dental implants, a novel technology based on the bioelectric effect (BE) to combat inflammation has emerged. A BE-integrated toothbrush was developed and clinically tested on patients with dental implants (N = 36). Our findings revealed a significant average plaque index reduction of 67% with BE technology compared with that at baseline (P < 0.05), whereas non-BE did not yield statistical significance even after 4 weeks of use (P > 0.05). The bleeding index demonstrated a 59% average reduction in all surfaces with BE technology (P < 0.05), whereas the non-BE group exhibited no significant change. Substantial reductions in total plaque and bleeding indices suggest that using BE toothbrushes can help effectively remove oral biofilms and treat bleeding symptoms.
Enhanced antioxidant, tyrosinase inhibition, and anti-inflammatory activities of Praeparatum mungo and three of its derivatives
Authors: Chang Tzu-Chin , Cao Jie-Ling , Lin Yung-Sheng , Huang Shu-Ling 
Published: December 2023

AbstractThe main objective of this study is to explore the functions of Praeparatum mungo (PM) and three of its derivatives, Praeparatum mungo/turmeric (PM/T), Praeparatum mungo/bromelain (PM/B), and Praeparatum mungo/inorganic elements (PM/IE). The results indicated that additives included in the fermentation process of PM enhanced PM’s antioxidant properties. PM/B exhibited the highest total phenolic content (19.18 ± 0.46 mg gallic acid equivalent/g), DPPH free radical scavenging activity, and ferric reducing power. PM/IE exhibited the highest ABTS free radical scavenging activity and chelating ferrous ion activity. PM/T exhibited the best inhibitory tyrosinase activity. The 625 μg/mL PM extract can extensively reduce nitric oxide production of RAW264.7 macrophages stimulated by 1 μg/mL LPS and exhibited no cytotoxicity for anti-inflammatory applications. Additives in PM natural fermentation process can enhance antioxidant, tyrosinase inhibition, and anti-inflammatory properties of PM for future applications.
A case study on new high-strength temporary support technology of extremely soft coal seam roadway
Authors: Xu Zhijun , Li Chong , Cao Yue , Tai Lianhai , Han Jun 
Published: December 2023

AbstractOne of the main challenges in excavating roadways is implementing temporary supports that are powered by hydraulics and have high strength. The current temporary support system lacks active support and often causes separation between the top plate and the layer below. It is crucial to control the initial separation of the roadway roof for the stability of the surrounding rock, especially on roadways with loose and soft rock. This research focuses on the A4027 return airway in Sail Six Mine. The issues with the temporary support system in this airway have been identified. The concept and principle of using hydraulically driven, high-strength temporary support technology are proposed. A mechanical analysis model is created to study the stacked roof in the temporary support region, and the critical conditions for delamination of the top plate are determined. The relationship between the delamination difficulty parameter Q, the distance between temporary supports L, and the strength of the temporary supports q is quantified. Numerical simulation using Flac3d is used to model the relationship between the strength of the temporary supports and the deformation and stress of the rock on the roof. The overall strength of the temporary supports for the A4027 return airway is determined to be 10 kN/m2, with a distance of 2 m between the temporary supports. Hydraulically driven, high-strength temporary support devices are developed and tested for their strength. Field trials are conducted as well. The results show that the initial separation of the top plate is improved and that the support effect in the temporary support region is significant. The maximum separation of the top plate during excavation is only 34 mm, and the sinking of the top plate does not exceed 68 mm. This effectively limits the deformation of the surrounding rocks in the very soft coal seam, providing valuable insights for other roadways with similar conditions.
The impact of community nucleic acid testing on infection in residential compounds during a city-wide lockdown
Authors: Jia Zhenzhen , Hu Jianqiang , Lian Teng , Qian Lixian , Yu Wen , Zhang Cheng 
Published: December 2023

AbstractThe question of whether community nucleic acid testing contributes to an increase in infections within residential compounds has not been definitively answered. Shanghai, one of the largest cities in China, conducted city-wide community testing during its lockdown from late March to May 2022. This situation provided a unique opportunity to examine the effect of community testing on infection rates, as the lockdown largely eliminated confounding factors such as citizen mobility. In our study, based on a survey of 208 residential compounds in Shanghai and the daily infection data during the lockdown period, we found a significant correlation between community testing and infection risk in these compounds. However, after addressing potential issues of reverse causality and sampling bias, we found no significant causal link between community testing and infection risk. Furthermore, we discovered that increased awareness of mask-wearing correlated with a decrease in infections within the residential compounds during community testing. This suggests that the perceived correlation between community testing and infection risk may be confounded by residents’ adherence to mask-wearing practices. Our findings emphasize the need for public health decision-makers to reinforce the importance of mask-wearing during community testing, as a means to prevent infections among citizens.
Chemokine- and chemokine receptor-based signature predicts immunotherapy response in female colorectal adenocarcinoma patients
Authors: Zhu Wenjie , Wu Changlei , Hu Shiqi , Liu Sicheng , Zhao Shimin , Zhang Dongdong , Qiu Guisheng , Cheng Xiufeng , Huang Jun 
Published: December 2023

AbstractThe clinical significance and comprehensive characteristics of chemokines and chemokine receptors in female patients with advanced colorectal adenocarcinoma have not ever been reported. Our study explored the expression profiles of chemokines and chemokine receptors and constructed a chemokine- and chemokine receptor-based signature in female patients with advanced colorectal adenocarcinoma. Four independent cohorts containing 1335 patients were enrolled in our study. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) analyses were performed to construct the signature. CIBERSORT was used to evaluate the landscape of immune cell infiltration. Thirty-two pairs of tissue specimens of female advanced colorectal cancer (CRC) patients and two CRC cell lines were used to validate the signature in vitro. Quantitative real-time PCR and western blotting were performed to validate the mRNA and protein expression levels of signature genes. EdU and colony formation assays were performed to examine proliferative ability. Transwell and wound healing assays were used to evaluate cell invasion and migration capacity. During the signature construction and validation process, we found that the signature was more applicable to female patients with advanced colorectal adenocarcinoma. Hence, the subsequent study mainly focused on the particular subgroup. Enrichment analyses revealed that the signature was closely related to immunity. The landscape of immune cell infiltration presented that the signature was significantly associated with T cells CD8 and neutrophils. Gene set enrichment analysis (GSEA) confirmed that the high-risk group was chiefly enriched in the tumor-promoting related pathways and biological processes, whereas the low-risk group was mainly enriched in anti-tumor immune response pathways and biological processes. The signature was closely correlated with CTLA4, PDL1, PDL2, TMB, MSI, and TIDE, indicating that our signature could serve as a robust biomarker for immunotherapy and chemotherapy response. ROC curves verified that our signature had more robust prognostic power than all immune checkpoints and immunotherapy-related biomarkers. Finally, we used 32 pairs of tissue specimens and 2 CRC cell lines to validate our signature in vitro. We first provided a robust prognostic chemokine- and chemokine receptor-based signature, which could serve as a novel biomarker for immunotherapy and chemotherapy response to guide individualized treatment for female patients with advanced colorectal adenocarcinoma.
The influence of variations in actual evapotranspiration on drought in China's Southeast River basin
Authors: Hong Sheng , Deng Haijun , Zheng Zhouyao , Deng Yu , Chen Xingwei , Gao Lu , Chen Ying , Liu Meibing 
Published: December 2023

AbstractRevealing changes in actual evapotranspiration is essential to understanding regional extreme hydrological events (e.g., droughts). This study utilized the Global Land Evaporation Amsterdam Model (GLEAM) to analyse the spatial and temporal characteristics of actual evapotranspiration over 40 years in the Southeast River basin of China. The relationship between changes in actual evapotranspiration and the drought index was quantified. The results indicated a significant increase in actual evapotranspiration in the Southeast River basin from 1981 to 2020 (2.51 mm/year, p < 0.01). The actual evapotranspiration components were dominated by vegetation transpiration (73.45%) and canopy interception (18.26%). The actual evapotranspiration was closely related to the normalised difference vegetation index (r = 0.78, p < 0.01), and vegetation changes could explain 10.66% of the increase of actual evapotranspiration in the Southeast River basin since 2000. Meanwhile, actual evapotranspiration and standardised precipitation evapotranspiration index (SPEI) showed a highly significant negative spatial correlation, with a Moran's I index of − 0.513. The rise in actual evapotranspiration is an important trigger factor for seasonal droughts in the region. Therefore, these results help deepen the understanding of hydro-climatic process changes in the southeastern coastal region of China.
The stridulatory organ in Opetiidae and Phoridae (Diptera) and phylogenetic implications for the evolution of higher flies
Authors: Brown Brian V. , de Souza Amorim Dalton 
Published: December 2023

AbstractStridulatory sound-making organs evolved in a group of flies—the family Phoridae—by modifications of the microstructure of foreleg segments present in the shared ancestor of the clade (Phoridae + Opetiidae). The opetiids are the only group amongst the lower Cyclorrhapha in which plausible homologous structures could be found, though in a less derived condition. On the forefemur of Opetia there are numerous elongate, flattened microtrichia that in basal phorids are organized into a curved linear group (the scraper) which are scraped against a curved, ridged carina on the forecoxa (the file). The file was possibly derived from an extremely unusual set of three setae that have transverse sculpturing and sockets that limit lateral motion, and which are distributed across the opetiid forecoxa. In some phorid lineages, these setae seem to be fused into the forecoxa forming the linear ridged surface against which the scraper on the forefemur could be moved. The relationship between opetiids and phorids dates back to the Cretaceous, and this pattern of file and scraper can be clearly seen in some 100 mya Myanmar amber phorid fly fossils. These structures shared between opetiids and phorids suggest that these two families may be sister groups amongst the Platypezoidea. Different modifications of the forelegs of other higher flies may have similar roles.
Establishment and study of a rat internal haemorrhoid model
Authors: Ke Minhui , Huang Shuyan , Lin Haorong , Xu Zhenguo , Li Xueyu , Li Zuanfang , Chen Feng , Wu Huasong 
Published: December 2023

AbstractTo establish a relatively stable internal haemorrhoid model in rats. A total of 48 SPF SD rats were selected and randomly divided into a blank group of 16 and a model group of 32. The model was created by croton oil-mixed liquid stimulation combined with standing and swimming experiments, and the modelling times were 1 week and 2 weeks, respectively. By observing the symptoms and signs of rats, pathological morphology and immunohistochemical staining of anorectal tissue, anorectal laser speckle blood-flow imaging and defecation contrast, etc., the effect of different modelling times was evaluated. The stability of the model was evaluated after feeding for 2 weeks. Both model-formation times caused rats to produce local symptoms of tissue bulging in the haemorrhoid area. Microscopy showed that the rectal submucosal interstitial blood vessels were dilated, and inflammatory cell infiltration and other manifestations were observed. Laser speckle blood-flow imaging revealed increased anorectal blood perfusion and capillary dilatation, and defecography showed a longitudinal and continuous rectal mucosa. After 2 weeks of normal feeding, lifting of the haemorrhoidal tissue was still present. The effect of modelling for 1 week was most in line with the clinical manifestations of internal haemorrhoids. The 1-week modelling scheme in this study can effectively establish a rat internal haemorrhoid model that closely approximates clinical internal haemorrhoid symptoms and pathological manifestations. The operation is simple, the success rate is high, and the model has certain stability. This model can be used as an important basis for studying various treatment methods for internal haemorrhoids.
Self-paced graph memory for learner GPA prediction and it’s application in learner multiple evaluation
Authors: Yun Yue , Cao Ruoqi , Dai Huan , Zhang Yupei , Shang Xuequn 
Published: December 2023

AbstractA scientific and rational evaluation of teaching is essential for personalized learning. In the current teaching assessment model that solely relies on Grade Point Average (GPA), learners with different learning abilities may be classified as the same type of student. It is challenging to uncover the underlying logic behind different learning patterns when GPA scores are the same. To address the limitations of pure GPA evaluation, we propose a data-driven assessment strategy as a supplement to the current methodology. Firstly, we integrate self-paced learning and graph memory neural networks to develop a learning performance prediction model called the self-paced graph memory network. Secondly, inspired by outliers in linear regression, we use a t-test approach to identify those student samples whose loss values significantly differ from normal samples, indicating that these students have different inherent learning patterns/logic compared to the majority. We find that these learners’ GPA levels are distributed across different levels. Through analyzing the learning process data of learners with the same GPA level, we find that our data-driven strategy effectively addresses the shortcomings of the GPA evaluation model. Furthermore, we validate the rationality of our method for student data modeling through protein classification experiments and student performance prediction experiments, it ensuring the rationality and effectiveness of our method.
First-passage-time statistics of growing microbial populations carry an imprint of initial conditions
Authors: Jones Eric W. , Derrick Joshua , Nisbet Roger M. , Ludington William B. , Sivak David A. 
Published: December 2023

AbstractIn exponential population growth, variability in the timing of individual division events and environmental factors (including stochastic inoculation) compound to produce variable growth trajectories. In several stochastic models of exponential growth we show power-law relationships that relate variability in the time required to reach a threshold population size to growth rate and inoculum size. Population-growth experiments inE. coliandS. aureuswith inoculum sizes ranging between 1 and 100 are consistent with these relationships. We quantify how noise accumulates over time, finding that it encodes—and can be used to deduce—information about the early growth rate of a population.
Friction taper stitch welding of a duplex stainless steel
Authors: Rapaka Ram , de Lima Lessa Cleber Rodrigo , Vieira Braga Lemos Guilherme , Pacheco Figueiredo Arlan , Vicharapu Buchibabu , Clarke Thomas , De Amitava 
Published: December 2023

AbstractFriction taper stitch welding (FTSW) is a novel technique that uses multiple inserts to conceal surface crack in a given substrate. The inserts are rotated and forced to fill the crack as plasticized material, and forge with the substrate in solid-state. The process is well suited for alloys such as duplex stainless steel, which suffers degradation of properties during fusion welding. A detailed experimental and theoretical investigation is presented here on FTSW of a duplex stainless steel (DSS). The experimental results show the presence of a ferrite-rich phase along the interface. The results computed by the numerical process model reveal a direct influence of thermal cycle in the amount of ferrite along the joint interface. The welded joint shows near homogeneous structure and properties similar to those of the substrate.
Countenance and implication of Β-sitosterol, Β-amyrin and epiafzelechin in nickel exposed Rat: in-silico and in-vivo approach
Authors: Zahid Sara , Malik Arif , Waqar Suleyman , Zahid Fatima , Tariq Nusrat , Khawaja Ali Imran , Safir Waqas , Gulzar Faisal , Iqbal Javeid , Ali Qurban 
Published: December 2023

AbstractThe detrimental impact of reactive oxygen species on D.N.A. repair processes is one of the contributing factors to colon cancer. The idea that oxidative stress may be a significant etiological element for carcinogenesis is currently receiving more and more support. The goal of the current study is to evaluate the anti-inflammatory and anticancer activity of three powerful phytocompounds—sitosterol, amyrin, and epiafzelechin—alone and in various therapeutic combinations against colon cancer to identify the critical mechanisms that mitigate nickel's carcinogenic effect. To evaluate the ligand–protein interaction of four selected components against Vascular endothelial growth factor (VEGF), Matrix metalloproteinase-9 (MMP9) inhibitor and Interleukin-10 (IL-10) molecular docking approach was applied using PyRx bioinformatics tool. For in vivo analysis, hundred albino rats were included, divided into ten groups, each containing ten rats of weight 160–200 g. All the groups were injected with 1 ml/kg nickel intraperitoneally per week for three months, excluding the negative control group. Three of the ten groups were treated with β-sitosterol (100 mg/kg b wt), β-amyrin (100 mg/kg b wt), and epiafzelechin (200 mg/kg b wt), respectively, for one month. The later four groups were fed with combinatorial treatments of the three phyto compounds for one month. The last group was administered with commercial drug Nalgin (500 mg/kg b wt). The biochemical parameters Creatinine, Protein carbonyl, 8-hydroxydeoxyguanosine (8-OHdG), VEGF, MMP-9 Inhibitor, and IL-10 were estimated using ELISA kits and Glutathione (G.S.H.), Superoxide dismutase (S.O.D.), Catalase (C.A.T.) and Nitric Oxide (NO) were analyzed manually. The correlation was analyzed through Pearson’s correlation matrix. All the parameters were significantly raised in the positive control group, indicating significant inflammation. At the same time, the levels of the foresaid biomarkers were decreased in the serum in all the other groups treated with the three phytocompounds in different dose patterns. However, the best recovery was observed in the group where the three active compounds were administered concomitantly. The correlation matrix indicated a significant positive correlation of IL-10 vs VEGF (r = 0.749**, p = 0.009), MMP-9 inhibitor vs SOD (r = 0.748**, p = 0.0 21). The study concluded that the three phytocompounds β-sitosterol, β-amyrin, and epiafzelechin are important anticancer agents which can target the cancerous biomarkers and might be used as a better therapeutic approach against colon cancer soon.
Comparative assessment of direct absorption solar collector performance in different climates
Authors: Heyhat Mohammad Mahdi , Abbood Mohammed Qasim Jawad , Ahbabi Saray Jabraeil , Mokhtari Ardekani Abolghasem 
Published: December 2023

AbstractEnergy supply and environmental protection by reducing pollutants are among the main challenges these days. As a clean and sustainable source, solar energy is capable of generating thermal and electrical power. In this regard, Iraq is one of the regions with high solar energy harvesting potential. A numerical model was developed and validated by experimental findings in MATLAB software. This model, which also included geometrical and optical characteristics, was developed using information from four cities representative of different climates in Iraq: Baghdad, Samawa, Mosul, and Al-Qa'im. This study examined the effects of climate on the performance of direct absorption parabolic solar collectors used for energy production in Iraq. According to the results, solar collectors in Samaveh provide the highest thermal energy efficiency (up to 66.5%). Even thoth, the highest exergy efficiency is found in Al-Qa'im (36.21%). From an environmental point of view, the collector in Al-Qa'im has the highest CO2 mitigation (2.73 kg per m2 of collector) every year. As compared to other cities, Al-Qa'im and Samawah have a high thermal efficiency and solar intensity, which can lead to more water and energy savings.