bluuebunny/crossref_metadata_2025_split huggingface dataset:
Congenital Mesoblastic Nephroma Mimic Wilms Tumor on 18F-FDG PET/CT and PET/MR
Authors: Hu Wenzhu , Qin Chunxia , Shao Fuqiang , Li Mengting , Lan Xiaoli 
Published: January 2024

Abstract
Congenital mesoblastic nephroma is an extremely rare, low-grade malignant renal tumor in children. A 10-month-old boy and a 4-month-old girl were admitted to our hospital with a huge abdominal mass. For staging of the mass, 18F-FDG PET/CT and PET/MR were performed showing a huge heterogeneous abdominal mass accompanied by extensive heterogeneous aggregation. Both of them were highly suspected to be Wilms tumor, the most common renal malignant tumor in children. However, histopathological examination after surgery confirmed congenital mesodermal nephroma.
Modified Brain Injury Guidelines for preinjury anticoagulation in traumatic brain injury: An opportunity to reduce health care resource utilization
Authors: Gallagher Shea P. , Capacio Benedict A. , Rooney Alexandra S. , Schaffer Kathryn B. , Calvo Richard Y. , Sise C. Beth , Krzyzaniak Andrea , Sise Michael J. , Bansal Vishal , Biffl Walter L. , Martin Matthew J. 
Published: January 2024

INTRODUCTION
The Brain Injury Guidelines (BIG) stratify patients by traumatic brain injury (TBI) severity to provide management recommendations to reduce health care resource burden but mandates that patients on anticoagulation (AC) are allocated to the most severe tertile (BIG 3). We sought to analyze TBI patients on AC therapy using a modified BIG model to determine if this population can offer further opportunity for safe reductions in health care resource utilization.

METHODS
Patients 55 years or older on AC with traumatic intracranial hemorrhage (ICH) from two centers were retrospectively stratified into BIG 1 to 3 risk groups using modified BIG criteria excluding AC as a criterion. Intracranial hemorrhage progression, neurosurgical intervention (NSI), death, and worsened discharge status were compared.

RESULTS
A total of 221 patients were included, with 23%, 29%, and 48% classified as BIG 1, BIG 2, and BIG 3, respectively. The BIG 3 cohort had a higher rate of AC reversal agents administered (66%) compared with the BIG 1 (40%) and BIG 2 (54%) cohorts (p < 0.01), as well as ICH progression discovered on repeat head computed tomography (56% vs. 38% vs. 26%, respectively; p < 0.001). No patients in the BIG 1 and 2 cohorts required NSI. No patients in BIG 1 and 3% of patients in BIG 2 died secondary to the ICH. In the BIG 3 cohort, 16% of patients required NSI and 26% died. Brain Injury Guidelines 3 patients had 15 times the odds of mortality compared with BIG 1 patients (p < 0.01).

CONCLUSION
The AC population had higher rates of ICH progression than the BIG literature, but this did not lead to more NSI or mortality in the lower tertiles of our modified BIG protocol. If the modified BIG used the original tertile management on our population, then NS consultation may have been reduced by up to 52%. These modified criteria may be a safe opportunity for further health care resource and cost savings in the TBI population.

LEVEL OF EVIDENCE
Prognostic and Epidemiological; Level IV.
Use of Transesophageal Echocardiography During Orthotopic Liver Transplantation: Simplifying the Procedure
Authors: Arcas-Bellas José J. , Siljeström Roberto , Sánchez Cristina , González Ana , García-Fernández Javier 
Published: January 2024

The intraoperative management of patients undergoing orthotopic liver transplantation (OLT) is influenced by the cardiovascular manifestations typically found in the context of end-stage liver disease, by the presence of concomitant cardiovascular disease, and by the significant hemodynamic changes that occur during surgery.
Hypotension and intraoperative blood pressure fluctuations during OLT are associated with liver graft dysfunction, acute kidney failure, and increased risk of 30-d mortality. Patients also frequently present hemodynamic instability due to various causes, including cardiac arrest.
Recent evidence has shown transesophageal echocardiography (TEE) to be a useful minimally invasive monitoring tool in patients undergoing OLT that gives valuable real-time information on biventricular function and volume status and can help to detect OLT-specific complications or situations. TEE also facilitates rapid diagnosis of life-threatening conditions in each stage of OLT, which is difficult to identify with other types of monitoring commonly used.
Although there is no consensus on the best approach to intraoperative monitoring in these patients, intraoperative TEE is safe and useful and should be recommended during OLT, according to experts, for assessing hemodynamic changes, identifying possible complications, and guiding treatment with fluids and inotropes to achieve optimal patient care.
Optic Disc Edema Is an Under-Recognized Feature of Birdshot Chorioretinitis
Authors: Sabapathypillai Sharon , Miller Victoria J. , Shakoor Akbar , Palestine Alan G. , Thorne Jennifer E. , Goldstein Debra A. , Gaudio Paul A. , Goldberg Naomi , Vitale Albert , Schlaen Ariel , Thomas Akshay , Merrill Pauline T. , Raiji Veena , Lin Phoebe , Oliver Armando L. , Moorthy Ramana S. , Chandra Gaurav , Carreno Ester , Smith Wendy M. , Van Stavern Gregory , Hassman Lynn M. 
Published: January 2024

Background:
Optic disc edema is a feature of many ophthalmic and neurologic conditions. It remains an underappreciated feature of birdshot chorioretinitis (BSCR), leading to delay in diagnosis and treatment. The purpose of our study was to identify clinical features that are concomitant with optic disc edema and suggest a diagnosis of BSCR.

Methods:
Retrospective multicenter case series of 29 patients who were referred to a neuro-ophthalmologist or uveitis specialist for evaluation of disc edema and were ultimately diagnosed with BSCR.

Results:
Fifty-four eyes of 30 patients, from the practices of 15 uveitis specialists, met the eligibility criteria. In addition to disc edema, concomitant features in all patients included vitritis, chorioretinal lesions, and retinal vasculitis. Visual recovery to 20/40 or better occurred in 26 of 29 patients. Visual acuity remained 20/100 or worse in 2 patients previously diagnosed with idiopathic intracranial hypertension, 1 patient previously diagnosed with optic neuritis, and 1 patient for whom treatment was delayed for years, leading to optic disc atrophy.

Conclusions:
Optic disc edema is a presenting feature in some cases of BSCR. A diagnosis of BSCR should be considered when disc edema occurs with vitritis, chorioretinal inflammation, and retinal vasculitis. Patients should be referred to a uveitis specialist for treatment.
Multiple Sclerosis Stages and their Differentially Expressed Genes: A Bioinformatics Analysis
Authors: Alaya Faten , Baraket Ghada , Adediran Daniel A. , Cuttler Katelyn , Ajiboye Itunu , Kivumbi Mark T. , Sitharam Nikita , Awe Olaitan I. 
Published: January 2024

AbstractMultiple Sclerosis (MS) is an inflammatory, chronic, autoimmune, and demyelinating disease of the central nervous system. MS is a heterogeneous disease with three main clinical forms, affecting the progression and therefore the treatment of the disease. Thus, finding key genes and microRNAs (miRNA) associated with MS stages and analyzing their interactions is important to better understand the molecular mechanism underlying the occurrence and the evolution of MS. Based on publicly available datasets of mRNA and miRNA expression profiles, differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) between patients with different stages of MS and healthy controls and between relapsing and remitting phases of RRMS were determined using Deseq2 and GEO2R tools. We then analyzed miRNA-mRNA regulatory interactions and gene ontology for the DEGs.Based on miRNA-mRNA regulatory interactions, we identified potential biomarkers of RRMS, 13 upregulated miRNA regulators of 30 downregulated genes and 17 downregulated miRNA regulators of 32 upregulated genes. We also identified 9 downregulated miRNA regulators of 12 upregulated genes as potential biomarkers of SPMS.Our study findings highlight some key protein-coding genes and miRNAs that are involved in the occurrence and evolution of MS.
Analysis of B-cell receptor repertoire to evaluate immunogenicity of monovalent Omicron XBB.1.5 mRNA vaccines
Authors: Funakoshi Yohei , Yakushijin Kimikazu , Ohji Goh , Matsutani Takaji , Doi Kazuhiko , Sakai Hironori , Sasaki Tomoki , Kusakabe Takahiro , Matsumoto Sakuya , Saito Yasuyuki , Kawamoto Shinichiro , Yamamoto Katsuya , Koyama Taiji , Nagatani Yoshiaki , Kurata Keiji , Kimbara Shiro , Imamura Yoshinori , Kiyota Naomi , Ito Mitsuhiro , Minami Hironobu 
Published: January 2024

ABSTRACTMonovalent Omicron XBB.1.5 mRNA vaccines (BNT162b2 XBB.1.5 and mRNA- 1273.815) were newly developed and approved by the FDA in Autumn 2023 for preventing COVID-19. However, clinical efficacy for these vaccines is currently lacking. We previously established the Quantification of Antigen-specific Antibody Sequence (QASAS) method to assess the response to SARS-CoV-2 vaccination at the mRNA level using B-cell receptor (BCR) repertoire assay and the Coronavirus Antibody Database (CoV-AbDab). Here, we used this method to evaluate the immunogenicity of monovalent XBB.1.5 vaccines in healthy volunteers. We analyzed repeated blood samples before and after vaccination for the BCR repertoire to assess BCR/antibody sequences that matched SARS-CoV-2-specific sequences in the database. The number of matched unique sequences and their total reads quickly increased 1 week after vaccination. Matched sequences included those bound to the Omicron strain and Omicron XBB sublineage. The antibody sequences that can bind to the Omicron strain and XBB sublineage revealed that the monovalent XBB.1.5 vaccines showed a stronger response than previous vaccines or SARS-CoV-2 infection before the emergence of XBB sublineage. The QASAS method was able to demonstrate the immunogenic effect of monovalent XBB.1.5 vaccines for the 2023-2024 COVID-19 vaccination campaign.
Changes in sensorimotor network dynamics in resting-state recordings in Parkinson’s Disease
Authors: Kohl Oliver , Gohil Chetan , Zokaeia Nahid , Hu Michele T. , Nobrea Anna C. , Woolrich Mark , Quinn Andrew 
Published: January 2024

AbstractNon-invasive recordings of magnetoencephalography (MEG) have been used for developing biomarkers for neural changes associated with Parkinson’s Disease (PD) but have yielded inconsistent findings. Here, we investigated whether analysing motor cortical activity within the context of large-scale brain network activity provides a more sensitive marker of changes in PD using MEG.We extracted motor cortical beta power and beta bursts from resting-state MEG scans of individuals with PD (N=28) and well-matched healthy controls (N=36). To situate beta bursts in their brain network contexts, we used a time-delay embedded Hidden Markov Model (TDE-HMM) to extract brain network activity and investigated co-occurrence patterns between brain networks and beta bursts.PD was associated with decreased beta power in motor cortex and decreased occurrences of the sensorimotor network, while motor cortical beta-burst dynamics were not changed. By comparing conventional burst and large-scale network occurrences, we observed that motor beta bursts occurred during both sensorimotor network and non-sensorimotor network activations. When using the large-scale network information provided by the TDE-HMM to focus on bursts that were active during sensorimotor network activations, significant decreases in burst dynamics could be observed in individuals with PD.In conclusion, our findings suggest that decreased motor cortical beta power in PD is prominently associated with changes in sensorimotor network dynamics using MEG. Thus, investigating large-scale networks or considering the large-scale network context of motor cortical activations may be crucial for identifying alterations in the sensorimotor network that are prevalent in PD, and might help resolve contradicting findings in the literature.HighlightsSensorimotor network occurrences are decreased in Parkinson’s Disease.Motor cortical beta bursts occur during both sensorimotor network and non-sensorimotor network activations.Focusing on motor beta bursts occurring during sensorimotor network activations enables for better discrimination between controls and individuals with PD.The spatiotemporal details provided by large-scale network analysis may help to overcome discrepancies found in the PD literature.
Inference of alveolar capillary network connectivity from blood flow dynamics
Authors: Schmid Kerstin , Olivares Andy L. , Camara Oscar , Kuebler Wolfgang M. , Ochs Matthias , Hocke Andreas C. , Fischer Sabine C. 
Published: January 2024

The intricate structure of the lungs is essential for the gas exchange within the alveolar region. Despite extensive research on the pulmonary vasculature, there are still unresolved questions regarding the connection between capillaries and the vascular tree. A major challenge is obtaining comprehensive experimental data that integrates morphological and physiological aspects.We propose a computational approach that combines data-driven 3D morphological modeling with computational fluid dynamics simulations. This method enables investigating the connectivity of the alveolar capillary network with the vascular tree based on the dynamics of blood flow. We developed 3D sheet-flow models to accurately represent the morphology of the alveolar capillary network and conducted computational fluid dynamics simulations to predict flow velocities and pressure distributions.Our approach focuses on leveraging functional features to identify the most plausible architecture of the system. For given capillary flow velocities and arteriole-to-venule pressure drops, we deduce details about arteriole connectivity. Preliminary connectivity analyses for non-human species indicate that their alveolar capillary network of a single alveolus is linked to at least two arterioles with diameters of 20 µm or a single arteriole with a minimum diameter of 30 µm.Our study provides insights into the structure of the pulmonary microvasculature by evaluating blood flow dynamics. This inverse approach represents a new strategy to exploit the intricate relationship between morphology and physiology, applicable to other tissues and organs. In the future, the availability of experimental data will play a pivotal role in validating and refining the hypotheses analyzed with our computational models.New and noteworthyThe alveolus is pivotal for gas exchange. Due to its complex morphology and dynamic nature, structural experimental studies are challenging. Computational modeling offers an alternative. We developed a databased 3D model of the alveolar capillary network and performed blood flow simulations within it. Choosing a novel perspective, we inferred structure from function. We systematically varied properties of vessels connected to our capillary network and compared simulation results with experimental data to obtain plausible vessel configurations.
Morphine-induced side effects can be differentially modulated by cannabidiol in male and female rats
Authors: Jesus Carlos Henrique Alves , Volpe Jaqueline , Sotomaior Bruna Bittencourt , Barbosa Maria Augusta Ruy , Ferreira Matheus Vinicius , Fiatcoski Fernanda , Genaro Karina , Crippa José Alexandre de Souza , Souto Dênio Emanuel Pires , da Cunha Joice Maria 
Published: January 2024

AbstractOpioid use disorder (OUD) is a public health problem that includes symptoms such as withdrawal syndrome and opioid-induced hyperalgesia (OIH). Currently, drugs to treat side effects of opioids also have undesirable effects, which lead to limitations. This study investigated the effect of a treatment with cannabidiol (CBD) in morphine-induced hyperalgesia and withdrawal signs in morphine-dependent rats. Male and female rats were submitted to morphine-induced physical dependence protocol consisting of a twice daily treatment with morphine (7.89 mg/kg, 1ml/kg, s.c.) for 10 days. Nociception was measured using the hot plate test and morphine-induced thermal hyperalgesia was equally achieved following 7-10 days of morphine administration in male and female rats. Repeated treatment with CBD (30 mg/kg) was sufficient to prevent thermal hyperalgesia in male and female rats. Subsequently, rats received an acute administration of naloxone (2 mg/kg. s.c.), 90 minutes after the morphine treatment on day 11, the number of withdrawal signs was scored. Rats that received treatment exclusively with morphine presented significant withdrawal signs compared to control (Water). Morphine-dependent female rats showed a prevalent stereotyped behavior of rearing, whereas male rats had the sign of teeth chattering as the most preeminent. Treatment with CBD on day 11 partially attenuated the withdrawal signs in morphine-dependent male rats, but not female rats. Altogether, our data provide evidence of an anti-hyperalgesic effect of CBD in rats. Male and female rats treated chronically with morphine exhibited withdrawal signs in different ratios, indicating sex-differences in withdrawal behavior and CBD attenuated withdrawal signs in a sex-dependent manner.
A Non-targeted Proteomics Newborn Screening Platform for Genetic Disorders
Authors: Shibata Hirofumi , Nakajima Daisuke , Konno Ryo , Hijikata Atsuhi , Higashiguchi Motoko , Nihira Hiroshi , Shimodera Saeko , Miyamoto Takayuki , Nishitani-Isa Masahiko , Hiejima Eitaro , Izawa Kazushi , Takita Junko , Heike Toshio , Okamura Ken , Ohnishi Hidenori , Ishimura Masataka , Okada Satoshi , Yamashita Motoi , Morio Tomohiro , Kanegane Hirokazu , Imai Kohsuke , Nakamura Yasuko , Nonoyama Shigeaki , Uchiyama Toru , Onodera Masafumi , Nishikomori Ryuta , Ohara Osamu , Kawashima Yusuke , Yasumi Takahiro 
Published: January 2024

AbstractNewborn screening using dried blood spot (DBS) samples has made a substantial contribution to public healthcare by detecting patients with genetic disorders as neonates. Targeted measurements of nucleic acids and metabolites have played major roles in newborn screening to date, while the feasibility of new non-targeted approaches, including genome-wide DNA sequencing, has been explored. Here, we investigated the applicability of non-targeted quantitative proteomics analysis to newborn screening for genetic diseases. DBS protein profiling allowed monitoring of levels of proteins encoded by 2912 genes, including 1106 listed in the Online Mendelian Inheritance in Man database, in healthy newborn samples, and was useful in identifying patients with inborn errors of immunity by detecting reduced levels of disease causative proteins and cell-phenotypical alterations. Our results indicate that application of non-targeted quantitative protein profiling of DBS samples can forge a new path in screening for genetic disorders.
General and Anxiety-Linked Influence of Acute Serotonin Reuptake Inhibition on Neural Responses Associated with Attended Visceral Sensation
Authors: Livermore James J A , Skora Lina I , Adamatzky Kristian , Garfinkel Sarah N , Critchley Hugo D , Campbell-Meiklejohn Daniel 
Published: January 2024

AbstractSerotonin is known to have state-dependent modulatory influences on exteroceptive sensory processes and the processing of pain, but much less is known about its role in ordinary interoceptive processes and their relationships to affective states. This experiment compared the impact of a selective serotonin reuptake inhibitor (SSRI) (20mg CITALOPRAM), acutely increasing extracellular serotonin, to that of a PLACEBO on the neural processing of ordinary interoceptive sensations and the relationship of these influences to anxious states. Twenty-one healthy young volunteers completed the visceral interoceptive attention (VIA) task with each treatment, focusing attention on heart, stomach, or visual sensation control while scanned with functional magnetic resonance imaging (fMRI). The relative neural interoceptive response (IR) to heart sensation [heartminusvisual] and stomach sensation [stomachminusvisual] were compared between treatment conditions, controlling for general effects on sensory processing. CITALOPRAM reduced interoceptive processing in viscerosensory (bilateral posterior insular cortex, stomach-IR) and integrative/affective components (bilateral amygdala, stomach-IR and heart-IR) of known interoceptive pathways. We then searched for state-dependent modulatory effects of CITALOPRAM that varied with current levels of anxiety. The anterior insular cortex response to heart sensation increased with anxiety, replicating prior findings. This relationship disappeared on CITALOPRAM. Preliminarypost hocexploration found that CITALOPRAM’S effects on amygdalae response to stomach sensation predicted acute increases and decreases in anxiety. Overall, this evidence of general and state-dependent serotonergic influence advances our understanding of interoception, its regulation, and its relationship to anxious states.
Identifying the components of theShewanellaphage LambdaSo lysis system
Authors: Thöneböhn Svenja , Fischer Dorian , Kreiling Vanessa , Kemmler Alina , Oberheim Isabella , Hager Fabian , Schmid Nicole E , Thormann Kai M 
Published: January 2024

AbstractPhage-induced lysis of Gram-negative bacterial hosts usually requires a set of phage lysis proteins, a holin, an endopeptidase and a spanin system, to disrupt each of the three cell envelope layers. Genome annotations and previous studies identified a gene region in theShewanella oneidensisprophage LambdaSo, which comprises potential holin- and endolysin-encoding genes but lacks an obvious spanin system. By a combination of candidate approaches, mutant screening, characterization and microscopy we found that LambdaSo uses a pinholin/signal-anchor-release (SAR) endolysin system to induce proton-leakage and degradation of the cell wall. Between the corresponding genes we found that two extensively nested open reading frames encode a two-component spanin module Rz/Rz1. Unexpectedly, we identified another factor strictly required for LambdaSo-induced cell lysis, the phage protein Lcc6. Lcc6 is a transmembrane protein of 65 amino acid residues with hitherto unknown function, which acts at the level of holin in the cytoplasmic membrane to allow endolysin release. Thus, LambdaSo-mediated cell lysis requires at least four protein factors (pinholin, SAR-endolysin, spanin, Lcc6). The findings further extend the known repertoire of phage proteins involved in host lysis and phage egress.SignificanceFor the release of the assembled virions, phages have to breach the cell envelope. For Gram-negatives, this requires the disruption of three layers, the outer and inner membrane and the cell wall. In most cases, the lysis systems of phages infecting Gram-negatives comprises holins to disrupt or depolarize the membrane, thereby releasing or activating endolysins, which then degrade the cell wall. This, in turn, allows the spanins to become active and fuse outer and inner membrane, completing cell envelope disruption and allowing phage egress. Here we show that the presence of these three components may not be sufficient to allow cell lysis, implicating that also in known phages further factors may be required.