bluuebunny/crossref_metadata_2025_split huggingface dataset:
ERβ is a potent inhibitor of cell proliferation in the HCT8 human colon cancer cell line through regulation of cell cycle components
Authors: Martineti Valentina , Picariello Lucia , Tognarini Isabella , Sala Silvia Carbonell , Gozzini Alessia , Azzari Chiara , Mavilia Carmelo , Tanini Annalisa , Falchetti Alberto , Fiorelli Gianna , Tonelli Francesco , Brandi Maria Luisa 
Published: June 2005

Several strands of evidence indicate that oestrogens exert a protective role against the development of colon cancer through indirect and direct effects on colonic epithelium. Oestrogen receptor β (ERβ), the predominant ER subtype in human colon, is significantly decreased in colonic tumours compared with normal mucosa suggesting a potential role in the regulation of colon tumour growth.
To investigate this hypothesis we engineered human colon cancer ERα-negative HCT8 cells in order to obtain ERβ protein over-expression. Stably transfected cells were cloned and ERβ expression and functionality were monitored by RT-PCR, Western blotting and transactivation in an assay using oestrogen-responsive reporter constructs.
Over-expression of ERβ inhibited cell proliferation and increased cell adhesion in a ligand-independent manner. Its constitutive activation is possibly due to cross-talk with intracellular signalling pathways, as epidermal growth factor and IGF-I were able to induce ERβ transactivation.
A possible mechanism by which ERβ over-expression inhibits proliferation in HCT8 cells is by modulation of some key regulators of the cell cycle; there is a decrease in cyclin E and an increase in the cdk inhibitor p21CIP1. In fact, flow cytometry analysis provided evidence for blocking of the G1-S phase progression induced by ERβ over-expression. The magnitude of this effect was affected by the level of ERβ expression.
These results provide the first direct evidence that ERβ plays an important role in colon cancer as a regulator of cell proliferation through the control of key cell cycle modulators and arrest in G1-S phase transition. These findings are compatible with the hypothesis that the loss of ERβ expression could be one of the events involved in the development or progression of colon cancer.
Cover Picture: Synthesis of a Self‐Assembled Hybrid of Ultrananocrystalline Diamond and Carbon Nanotubes (Adv. Mater. 12/2005)
Authors: Xiao X. , Elam J. W. , Trasobares S. , Auciello O. , Carlisle J. A. 
Published: June 2005

AbstractThe cover shows self‐assembled hybrids of ultrananocrystalline diamond (UNCD) and carbon nanotubes (CNTs). These hybrids were successfully prepared by their simultaneous growth within an argon‐rich Ar/CH4 plasma, in work reported on p. 1496 by Carlisle and co‐workers. Various methods demonstrated the coexistence of UNCD and CNTs, and the capability of controlling the relative fraction and configuration of UNCD and CNTs in the hybrid material. This new synthesis pathway enables the development of new nanocarbons with unique mechanical, tribological, and electrochemical properties.
Dentatorubral‐pallidoluysian atrophy in three generations, with clinical courses from nearly asymptomatic elderly to severe juvenile, in an Australian family of Macedonian descent
Authors: Vinton Anita , Fahey Michael C. , O'Brien Terence J. , Shaw Janet , Storey Elsdon , McKinlay Gardner R.J. , Mitchell Peter J. , Du Sart Desirée , King John O. 
Published: June 2005

AbstractWe report a three‐generation Caucasian family of Macedonian origin with dentatorubral‐pallidoluysian atrophy (DRPLA), manifesting as very mild elderly onset, severe young adult onset, and severe childhood onset presentations in the three generations. The grandparental trinucleotide expansion size (52 repeats) is the smallest overtly pathogenic mutation yet reported. This 67‐year‐old man displayed only subtle neurological and cognitive deficits on formal examination and very slight signs on MRI. His son had developed a choreiform disorder at age 32 years, and by his 40s suffered severe dementia and motor decline. The grandson, the proband, presented as a teenager with progressive myoclonic epilepsy, dysarthria, ataxia, and cognitive decline, having manifesting learning difficulties from the age 5 years. Atrophin‐1 expansion sizes of 52, 57, and 66 repeats were demonstrated in the three patients, respectively. Given an absence of any other indicative history in the family, we speculate that the mutation may have expanded from a ‘high‐end’ normal allele to a pathogenic size at the grandfather's conception, or that one of his parents may have had a pathogenic mutation at the lowest end of the expanded range. © 2005 Wiley‐Liss, Inc.
Katalytische enantioselektive vinyloge Aldolreaktionen
Authors: Denmark Scott E. , Heemstra John R. , Beutner Gregory L. 
Published: June 2005

AbstractIm Jahre 1935 formulierte R. C. Fuson das Prinzip der Vinylogie, um zu erklären, wie sich der Einfluss einer funktionellen Gruppe an einer entfernten Position im Molekül bemerkbar macht, wenn diese über konjugierte Doppelbindungen mit der Gruppe verbunden ist. In polaren Reaktionen ermöglicht dieses Konzept die Erweiterung des elektrophilen oder nucleophilen Charakters einer funktionellen Gruppe über das π‐System einer Kohlenstoff‐Kohlenstoff‐Doppelbindung. Diese vinyloge Erweiterung führt im Fall der Aldolreaktion zum Einsatz “erweiterter” Dienolether, die von γ‐enolisierbaren α,β‐ungesättigten Carbonylverbindungen abgeleitet sind. Seit 1994 sind einige Methoden für die katalytische enantioselektive vinyloge Aldolreaktion beschrieben worden, mit denen man unterschiedliche Regio‐ (Orts‐), Enantio‐ und Diastereoselektivitäten erzielen kann. In diesem Aufsatz werden die Bandbreite und die Einschränkungen dieser Transformation sowie ihre Anwendung in der Naturstoffsynthese diskutiert.
Catalytic, Enantioselective, Vinylogous Aldol Reactions
Authors: Denmark Scott E. , Heemstra John R. , Beutner Gregory L. 
Published: June 2005

AbstractIn 1935, R. C. Fuson formulated the principle of vinylogy to explain how the influence of a functional group may be felt at a distant point in the molecule when this position is connected by conjugated double‐bond linkages to the group. In polar reactions, this concept allows the extension of the electrophilic or nucleophilic character of a functional group through the π system of a carbon–carbon double bond. This vinylogous extension has been applied to the aldol reaction by employing “extended” dienol ethers derived from γ‐enolizable α,β‐unsaturated carbonyl compounds. Since 1994, several methods for the catalytic, enantioselective, vinylogous aldol reaction have appeared, with which varying degrees of regio‐ (site), enantio‐, and diastereoselectivity can be attained. In this Review, the current scope and limitations of this transformation, as well as its application in natural product synthesis, are discussed.
Planung und Bauausführung des Al‐Sadd Stadions in Doha, Katar
Authors: Stroetmann Richard , Albert Andrej 
Published: June 2005

AbstractDas Stadion des Al‐Sadd Sports Club, dem renommiertesten Fußballklub des Emirats Katar, wurde in einem Zeitraum von rund 14 Monaten renoviert und in großen Teil en durch einen Neubau ersetzt. Der Beitrag berichtet über Besonderheiten bei der Planung und Bauausführung dieses Projekts. Auf die Stabilitätsberechnung des bogenförmigen Dachtragwerks wird detailliert eingegangen.
Lastansätze bei Tragfähigkeitsnachweisen im Holzbau
Authors: Schatz Tino 
Published: June 2005

AbstractDas neue Sicherheitskonzept ist mit dem Weißdruck der DIN 1052 auch im Holzbau Stand der Technik geworden. Gerade die Nachweise im Grenzzustand der Tragfähigkeit bringen dabei die deutlichsten Veränderungen gegenüber dem bisherigen Vorgehen. Der vorliegende Beitrag soll als Arbeitshilfe dienen und das Nachweisverfahren für die wichtigsten Lastfälle transparenter und übersichtlicher machen.
Zum Lastabtragungsmechanismus von vorgespannten Auftriebsankern
Authors: Dührkop Jan , Grabe Jürgen 
Published: June 2005

AbstractZur Beurteilung des Lastabtragungsmechanismus von Auftriebsankern wird ein einfaches Ersatzfedermodell erstellt, mit dem sich eine Ankerprüfung nach DIN 4125 simulieren läßt. Die Untersuchung zeigt, daß aus einem nicht freigespülten Bohrloch eine Einschränkung der Ankerwirkung als Auftriebsanker resultieren kann und diese durch eine Prüfung der freien Ankerlänge nicht unbedingt aufgedeckt wird.
Über den Einfluß der Kornverteilungskurve auf das dynamische und das kumulative Verhalten nichtbindiger Böden
Authors: Wichtmann Torsten , Triantafyllidis Theodor 
Published: June 2005

AbstractDieser Beitrag präsentiert experimentelle Untersuchungen an nichtbindigen Böden zum Einfluß der Kornverteilungskurve auf die dynamischen Bodenkenngrößen sowie die Akkumulation von bleibenden Verformungen unter zyklischer Belastung. Es wird gezeigt, daß der dynamische Schubmodul nicht vom mittleren Korndurchmesser beeinflußt wird, mit steigender Ungleichförmigkeit jedoch stark abnimmt. Für einen ungleichförmigen Boden überschätzen die gebräuchlichen empirischen Formeln den dynamischen Schubmodul um bis zu 70%. Auch die bleibenden Verformungen unter zyklischer Belastung hängen signifikant von der Kornverteilungskurve ab. Diese sind umso größer, je feinkörniger der Boden ist. Ungleichförmige Böden verdichten sich unter zyklischer Belastung wesentlich schneller als gleichförmige Böden.
Specific interaction of chalcone‐protein: Cardamonin binding site II on the human serum albumin molecule
Authors: He Wenying , Li Ying , Liu Jiaqin , Hu Zhide , Chen Xingguo 
Published: June 2005

AbstractCardamonin (2′,4′‐dihydroxy‐6′‐methoxychalcone), one of the main constituents from the seeds of Alpinia katsumadai Hayata, belongs to chalcone with its antibacterial, antiinflammatory and other important therapeutic activities of significant potency and low systemic toxicity. In this article, the interaction of cardamonin to human serum albumin (HSA) has been studied for the first time by spectroscopic methods including Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD), and UV‐absorption spectroscopy in combination with fluorescence quenching under physiological conditions with drug concentrations of 0.67–4.0 μM. The results of the spectroscopic measurements and the thermodynamic parameters obtained (the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be –25.312 and 7.040 J·mol–1·K–1 according to the van't Hoff equation) suggest that hydrophobic interaction is the predominant intermolecular forces stabilizing the complex, which is also in good agreement with the results of the molecule modeling study. The alterations of protein secondary structure in the presence of cardamonin in aqueous solution were quantitatively calculated by the evidence from CD and FTIR spectroscopes with reductions of α‐helices of about 20%, decreases of β‐sheet structures of about 14%, and increases of β‐turn structures of about 15%. The quenching mechanism and the number of binding sites (n ≈ 1) were obtained by fluorescence titration data. Fluorescent displacement measurements confirmed that cardamonin binds HSA on site II. In addition, the effects of common ions on the constants of the cardamonin–HSA complex were also discussed. 2005 Wiley Periodicals, Inc. Biopolymers 79: 48–57, 2005This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production
Authors: Choi Shin Sik , Rhee Won Jong , Park Tai Hyun 
Published: June 2005

AbstractTo produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum‐free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate‐induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate‐induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five‐fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase‐3 and other subsequent caspase reactions. © 2005 Wiley Periodicals, Inc.
A physiological product‐release model for baculovirus infected insect cells
Authors: Haas Richard , Nielsen Lars K. 
Published: June 2005

AbstractExisting models describe the product release from baculovirus infected insect cells as an unspecific protein leakage occurring in parallel with protein production. The model presented here shows that the observed product release of normally non‐secreted proteins can be described through cell death alone. This model avoids the implicit non‐physiological assumption of previous models that cells permeable to recombinant protein as well as trypan blue continue to produce protein. © 2005 Wiley Periodicals, Inc.
Paclitaxel and pegylated‐liposomal doxorubicin are both active in angiosarcoma
Authors: Skubitz Keith M. , Haddad Philip A. 
Published: June 2005

AbstractBACKGROUNDPaclitaxel has unique activity in angiosarcomas of the face and scalp, but its activity in angiosarcomas originating at other sites is less well defined. Paclitaxel and pegylated‐liposomal doxorubicin (PLD) are highly effective in Kaposi sarcoma (KS). Because of the efficacy of PLD in soft tissue sarcoma in general, and in KS in particular, coupled with potential similarities in KS and angiosarcoma, and the apparent activity of paclitaxel in angiosarcomas, the authors treated patients with angiosarcoma with either paclitaxel or PLD as initial chemotherapy.METHODSTo better define the efficacy of these agents in angiosarcoma, the authors reviewed their experience with paclitaxel and PLD in patients with angiosarcoma treated between 1994 and 2004.RESULTSThey identified seven patients with angiosarcoma treated with paclitaxel, and six treated with PLD. Only one patient in the series had an angiosarcoma of the scalp. Two patients receiving paclitaxel had received previous therapy with PLD, and four of six patients treated with PLD had previously received paclitaxel. Of the eight patients treated with paclitaxel, five had major responses (three had partial responses [PR] and two had complete disease remission [CR]) and three had progressive disease (PD). Of the 6 patients who received PLD, 3 had a PR for 6, 19, and >20 months, respectively, 2 had stable disease for 7 and 11 months, respectively, and 1 had PD.CONCLUSIONSThe current study demonstrated the activity of PLD (five of six patients experienced clinical benefit) and extended the data on paclitaxel in angiosarcoma, both of the face and scalp, as well as angiosarcoma originating at other sites. Cancer 2005. © 2005 American Cancer Society.
Ovarian carcinoma screening in women at intermediate risk
Authors: Kauff Noah D. , Hurley Karen E. , Hensley Martee L. , Robson Mark E. , Lev Gali , Goldfrank Deborah , Castiel Mercedes , Brown Carol L. , Ostroff Jamie S. , Hann Lucy E. , Offit Kenneth , Barakat Richard R. 
Published: June 2005

AbstractBACKGROUNDWomen with family histories suggestive of an increased risk of ovarian carcinoma who have not had a deleterious BRCA1 or BRCA2 mutation identified are commonly suggested to consider ovarian carcinoma screening with transvaginal ultrasound and/or assessment of CA 125 levels. Limited information is available regarding the impact of this approach on either quality of life (QOL) or need for invasive follow‐up in this group of women.METHODSFrom November 1999 to October 2002, 184 women at intermediate risk of ovarian carcinoma were enrolled in a prospective study. Participants were screened with twice yearly transvaginal ultrasound and CA 125 assessments. Impact on QOL was measured using the Mental Component Summary (MCS) score of the Medical Outcomes Studies Short Form‐36. Need for invasive follow‐up was determined by questionnaire and medical record review.RESULTSIn the current study, 135 participants underwent ≥ 1 follow‐up assessment. During a mean of 19.8 months of follow‐up, 12.9% of ultrasounds and 3.8% of CA 125 assessments were abnormal. The authors reported that 38.5% of participants had ≥ 1 abnormal ovarian screen that required a short interval follow‐up. Because of either abnormal bleeding or ultrasound abnormalities, 24% of participants underwent ≥ 1 endometrial sampling. Controlling for a history of breast carcinoma and menopausal status, abnormal ovarian screening results were associated with a decrease in MCS score (P = 0.034), whereas the need for endometrial sampling was not (P = 0.87).CONCLUSIONSOvarian carcinoma screening in women at intermediate risk was associated with a substantial rate of abnormal screen results, endometrial sampling, and in women with abnormal ovarian screening findings, a decrease in MCS scores. These findings may have important implications for women considering ovarian carcinoma screening and for the design of future ovarian carcinoma screening trials. Cancer 2005. © 2005 American Cancer Society.
Immunosuppression and infectious complications in patients with stage IV indolent lymphoma treated with a fludarabine, mitoxantrone, and dexamethasone regimen
Authors: Tsimberidou Apostolia‐Maria , Younes Anas , Romaguera Jorge , Hagemeister Fredrick B. , Rodriguez Maria A. , Feng Lei , Ayala Ana , Smith Terry L. , Cabanillas Fernando , McLaughlin Peter 
Published: June 2005

AbstractBACKGROUNDMyelosuppression and immunosuppression occur with purine analogs. The objective of the current study was to investigate the effects of combined fludarabine, mitoxantrone, and dexamethasone (FND) followed by interferon/dexamethasone on myelosuppression (absolute neutrophil counts), immunosuppression (CD4 and CD8 counts), and infectious complications in patients with previously untreated, Stage IV indolent lymphoma.METHODSSeventy‐three patients were treated. All patients received Pneumocystis carinii pneumonia (PCP) prophylaxis. CD4 and CD8 counts, serum immunoglobulin (Ig) levels, and neutrophil counts were correlated with infectious complications.RESULTSThe median follow‐up was 6.1 years. Sixty of 73 patients had CD4, CD8, or Ig measurements. The median baseline CD4 count was 764/μL: This CD4 level decreased to 238/μL at 1 year and to 264/μL at 2 years; and it rose to 431/μL by 3 years and to 650/μL at 4 years. CD8 counts did not change significantly. The median baseline serum IgG level was 989 mg/d, decreased to 536 mg/dL at 1 year and to 693 mg/dL at 2 years, and it rose to 949 mg/dL at 3 years and to 1080 mg/dL at 4 years. Fourteen patients (19%) developed Grade 3–4 infections, the majority during FND therapy with neutropenia and/or accompanied by CD4 counts < 200/μL. CD4, CD8, and neutrophil counts did not differ between patients who developed Grade 3–4 infections, Grade 1–2 infections, or no infections.CONCLUSIONSMost infections with FND occurred during FND, in the setting of neutropenia, often with concurrent low CD4 counts. The overall safety profile for FND was good. However, patients should be monitored for opportunistic infections, and prophylactic antibiotics are recommended, particularly against PCP. Cancer 2005. © 2005 American Cancer Society.
Influence of the time between surgery and radiotherapy on local recurrence in patients with lymph node‐positive, early‐stage, invasive breast carcinoma undergoing breast‐conserving surgery
Authors: Benchalal Mohamed , Le Prisé Elisabeth , de Lafontan Brigitte , Berton‐Rigaud Dominique , Belkacemi Yazid , Romestaing Pascale , Peignaux Karine , Courdi Adel , Monnier Alain , Montcuquet Philippe , Goudier Marie‐Josèphe , Marchal Christian , Chollet Philippe , Abadie‐Lacourtoisie Sophie , Datchary Jean , Veyret Corinne , Kerbrat Pierre 
Published: June 2005

AbstractBACKGROUNDRadiotherapy (RT) after breast‐conserving surgery (BCS) has produced significant reductions in ipsilateral breast carcinoma (BC) recurrence. It was shown previously that a delay in the initiation of RT resulted in a higher local recurrence (LR) rate. In the current retrospective analysis, the authors investigated whether the RT‐adjuvant therapy sequence modified local‐disease‐free survival (L‐DFS) after BCS in patients with early‐stage, lymph node‐positive BC.METHODSAmong 7 French Adjuvant Study Group trials, 1831 patients were assessable, including 475 patients who received RT directly after BCS (95 patients received no adjuvant therapy, and 380 patients received hormone therapy), 567 patients who received RT after the third chemotherapy (CT) cycle (250 patients received 1–3 courses, and 317 patients received 4–6 courses), and 789 patients received RT after the sixth CT cycle. In the 1356 patients who received CT, the regimens consisted of fluorouracil 500 mg/m2; epirubicin 50 mg/m2, 75 mg/m2, or 100 mg/m2; and cyclophosphamide 500 mg/m2in 83.5% of patients.RESULTSAfter a median follow‐up of 102 months, 214 patients (11.7%) developed LR. The 9‐year L‐DFS rates were 92.0%, 81.5%, and 87.4%, respectively (P < 0.0001). It was worse in patients who received 1–3 CT cycles (P= 0.02). Patients who received hormone therapy were less likely to develop LR (P= 0.02). In the multivariate analysis, the timing of RT was not associated with a higher rate of LR, whereas tumor size > 2 cm and no hormone therapy were prognostic factors.CONCLUSIONSIn the study population, there was no increase in the risk of LR when RT was delayed to deliver adjuvant CT. Prognostic factors were tumor size, and hormone therapy. The number of CT courses could modified this risk. Cancer 2005. © 2005 American Cancer Society.