bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engeletin alleviates depression‐like phenotype by increasing synaptic plasticity via the BDNF‐TrkB‐mTORC1 signalling pathway
Authors: Xu Yangyang , Zhang Jie , Yu Linyao , Zhang Wei , Zhang Yingtian , Shi Yaoqin , Zhang Shuping , Li Chunmei , Tian Jingwei 
Published: October 2023

AbstractMajor depressive disorder (MDD) is a severe mental disorder associated with high rates of morbidity and mortality. Current first‐line pharmacotherapies for MDD are based on enhancement of monoaminergic neurotransmission, but these antidepressants are still insufficient and produce significant side‐effects. Consequently, the development of novel antidepressants and therapeutic targets is desired. Engeletin, a natural Smilax glabra rhizomilax derivative, is a compound with proven efficacy in treating ischemic stroke, yet its therapeutic effects and mechanisms for depression remain unexplored. The effects of engeletin were assessed in the forced swimming test (FST) and tail suspension test (TST) in mice. Engeletin was also investigated in the chronic restraint stress (CRS) mouse model of depression with fluoxetine (FLX) as the positive control. Changes in prefrontal cortex (PFC) spine density, synaptic plasticity‐linked protein expressions and the brain‐derived neurotrophic factor (BDNF)‐tyrosine kinase B (TrkB)‐ mammalian target of rapamycin complex 1 (mTORC1) signalling pathway after chronic stress and engeletin treatment were then investigated. The TrkB and mTORC1 selective inhibitors, ANA‐12 and rapamycin, respectively, were utilized to assess the engeletin's antidepressive mechanisms. Our data shows that engeletin exhibited antidepressant‐like activity in the FST and TST in mice without affecting locomotor activity. Furthermore, it exhibited efficiency against the depression of CRS model. Moreover, it enhanced the BDNF‐TrkB‐mTORC1 pathway in the PFC during CRS and altered the reduction in dendritic spine density and levels of synaptic plasticity‐linked protein induced by CRS. In conclusion, engeletin has antidepressant activity via activation of the BDNF‐TrkB‐mTORC1 signalling pathway and upregulation of PFC synaptic plasticity.
Effect of ultrasound‐assisted thin bed drying for retaining the quality of red bell pepper and compare the predictive ability of the mathematical model with artificial neural network
Authors: Sasikumar Raju , Mangang Irengbam Barun , Vivek Kambhampati , Jaiswal Amit K. 
Published: October 2023

AbstractRed bell peppers (Capsicum annuum L.) are low in calories and high in nutrients, including vitamins A and C. Various factors such as weight loss, senescence, and microbial influence affect their quality during storage. To address this, the present study aims to preserve and retain the quality of red bell peppers using ultrasound‐assisted thin‐bed drying. The results were analyzed using an Artificial Neural Network (ANN) for more accurate prediction of variables involved in the process. Variations in moisture ratio and moisture content during drying were calculated and predicted. The Midilli model provided satisfactory curve fitting at an air velocity of 1.5 m/s, with R2 = 0.9993, χ2 = 0.0002, and RMSE = 0.0134. The two‐term and modified page models fit better with drying curves at air velocities of 2 and 2.5 m/s, with R2 = 0.9995, χ2 = 0.002, RMSE = 0.0016 and R2 = 0.9996, χ2 = 0.00003, RMSE = 0.00003, respectively. However, the trained standard backpropagation ANN algorithm demonstrated excellent predictive ability, outperforming the mathematical models with R2 = 0.9989 (training), MSE = 0.0001 (training), and R2 = 0.9996 (testing), MSE = 0.0002 (testing). Most importantly, the ultrasound‐assisted drying process retains the essential nutrients in red bell peppers, including vitamin C, carotenoids, polyphenols, and flavonoids, across various conditions. The antioxidant potential, as measured by DPPH and FRAP assays, remains largely unchanged compared to untreated samples. However, ABTS activity shows a significant difference at an air velocity of 1.5 m/s and temperatures of 60 and 70°C compared to the control sample.Practical applicationsIn terms of vitamins and antioxidants, red bell peppers are a great provider. Interestingly, capsaicin, the substance responsible for spiciness, is found in very little to no quantity in red bell peppers making it appealable to consumers. To achieve a certain level of processing, the quality of the dried product needs to be monitored while fulfilling the needs of consumers. Particularly, the quality‐determining parameters were vitamin C, total carotenoids, total polyphenols, total flavonoid content, DPPH, ABTS, and FRAP activity. These qualities were retained after drying with the potential novel combination of ultrasound with thin bed drying at low temperatures and air velocity. This means that the bioactive compounds responsible for improving the health of humans are readily available in dried form all year round. The drying kinetics were modeled, predicted, and compared with mathematical and ANN models.
Pedagogy of the great pandemic of the 21st century: Epistemic implications for individual psychology, family science, and psychotherapies
Authors: Medina Centeno Raúl 
Published: October 2023

AbstractAs an effect of the severe health crisis caused by COVID‐19, lockdown constituted a psychosocial laboratory that represented an experiment on a global scale. The lessons from the research findings in the pandemic environment have been of enormous importance to the disciplines of human behavior. This paper explores two themes evidenced in this context: people's psychological well‐being is underpinned by a socio‐emotional network, and the most frequent psychological symptoms experienced in the pandemic are a socio‐political problem; in particular it was observed that women reported greater discomfort than men in that period, showing with it that gender inequality remains a structural malaise. The conclusion points to two issues. First, an individualistic psychology is impossible without social content, especially in the collective affectivity, where the person participates. Second, psychotherapists should contemplate, in diagnosis and intervention, the socio‐emotional and political context in which the symptomatology appears. The focus of this article is based on systemic third‐order thinking, from where the socio‐economic and cultural structural order is closely linked to identity, well‐being, or personal discomfort.
The transcription factor FgStuA regulates virulence and mycotoxin biosynthesis via recruiting the SAGA complex in Fusarium graminearum
Authors: Xu Chaoyun , Wang Jing , Zhang Yueqi , Luo Yuming , Zhao Youfu , Chen Yun , Ma Zhonghua 
Published: October 2023

Summary
The conserved Spt‐Ada‐Gcn5‐Acetyltransferase (SAGA) complex controls eukaryotic transcription by modifying acetylation of histones. However, the mechanisms for this complex in regulating the transcription of target‐specific genes remain largely unknown in phytopathogenic fungi.
A filamentous fungal‐specific transcription factor FgStuA was identified to interact with the SAGA complex physically. The coordinative mechanisms of FgStuA with the SAGA complex in regulating secondary metabolism and virulence were investigated in Fusarium graminearum with genetic, biochemical and molecular techniques.
The transcription factor FgStuA binds to a 7‐bp cis‐element (BVTGCAK) of its target gene promoter. Under mycotoxin deoxynivalenol (DON) induction conditions, FgStuA recruits the SAGA complex into the promoter of TRI6, a core regulator of the DON biosynthesis gene cluster, leading to enhanced transcription of TRI6. During this process, we found that FgStuA is subject to acetylation by the SAGA complex, and acetylation of FgStuA plays a critical role for its enrichment in the TRI6 promoter. In addition, FgStuA together with the SAGA complex modulates fungal virulence.
This study uncovers a novel regulatory mechanism of a transcription factor, which recruits and interacts with the SAGA complex to activate specific gene expression in pathogenic fungi.
A pilot study for a practical screening tool for dementia: validation of 5‐minute cognitive test in a geriatric population
Authors: Hafızoğlu Merve , Okyar Baş Arzu , Şahiner Zeynep , Oytun Merve Güner , Atbaş Cansu , Karaduman Didem , İleri İbrahim , Balcı Cafer , Halil Meltem Gülhan , Cankurtaran Mustafa , Doğu Burcu Balam 
Published: October 2023

BackgroundThe aim of this study is to validate the Turkish version of the 5‐minute cognitive test (FCT) in a geriatric population.Materials and MethodIn total, 61 participants aged ≥65 years with normal cognitive functions, mild cognitive impairment (MCI), and early stage dementia were included. The FCT was compared to the standardised Mini Mental State Examination (MMSE) and the Qmci‐TR (quick mild cognitive impairment) test.ResultsTest reliability for the FCT was strong (Cronbach's α = 0.747). We demonstrated a strong correlation of FCT scores with MMSE and Qmci‐TR scores (respectively, r = 0.730, P < 0.001, r = 0.723, P < 0.001). The fact that the scores obtained in the dementia group were significantly lower also showed that the test can be used reliably in the differentiation of MCI and early dementia (P < 0.001).ConclusionsThe FCT is a brief, reliable, and valid cognitive screening test for screening dementia at early stages in a Turkish geriatric population.
A single nitrogen application maintains wheat yield and quality in dryland Mediterranean Vertisol
Authors: Fernandez‐Garcia Purificacion , Lopez‐Bellido Luis , Lopez‐Bellido Francisco J. , Lopez‐Bellido Rafael J. 
Published: October 2023

AbstractIn dryland Mediterranean Vertisols, split nitrogen (N) application is usual in wheat crops. Because of the high clay content of these soils and the low rainfall, a single application might offer ecological and economic benefits without yield loss. We conducted a 5‐year study as part of a long‐term experiment and compared the split application of N (50% at tillering, 50% at stem elongation phase) with a single application (tillering) in 10 cropping systems resulting from the combination of type of cultivation (no‐till and conventional tillage) and 2‐year crop rotations (wheat–wheat, wheat–fallow, wheat–chickpea, wheat–faba bean and wheat–sunflower). Depending on the system × crop rotation interaction, a single application of 100 or 150 kg N ha−1 maintained grain yield, protein content and apparent N recovery, with no effect on nitrate content compared with a traditional split application. The average grain yield did not differ between split and single application of N (3.27 and 3.23 Mg ha−1, respectively, for single and split application of 100 kg N ha−1 rate; 3.46 and 3.40 Mg ha−1, respectively, for single and split application of 150 kg N ha−1 rate). The soil nitrate concentration at pre‐seeding in the 0–0.3 m layer showed similar results, except in the case of the wheat–faba rotation, with lower nitrate concentration in the sub‐subplots of single application and no‐till. This work shows that adopting the single N application strategy in rotations on Mediterranean dryland Vertisols will reduce economic and environmental costs.
Genome sequences and population genomics provide insights into the demographic history, inbreeding, and mutation load of two ‘living fossil’ tree species of Dipteronia
Authors: Feng Yu , Comes Hans Peter , Chen Jun , Zhu Shanshan , Lu Ruisen , Zhang Xinyi , Li Pan , Qiu Jie , Olsen Kenneth M. , Qiu Yingxiong 
Published: October 2023

SUMMARY‘Living fossils’, that is, ancient lineages of low taxonomic diversity, represent an exceptional evolutionary heritage, yet we know little about how demographic history and deleterious mutation load have affected their long‐term survival and extinction risk. We performed whole‐genome sequencing and population genomic analyses on Dipteronia sinensis and D. dyeriana, two East Asian Tertiary relict trees. We found large‐scale genome reorganizations and identified species‐specific genes under positive selection that are likely involved in adaptation. Our demographic analyses suggest that the wider‐ranged D. sinensis repeatedly recovered from population bottlenecks over late Tertiary/Quaternary periods of adverse climate conditions, while the population size of the narrow‐ranged D. dyeriana steadily decreased since the late Miocene, especially after the Last Glacial Maximum (LGM). We conclude that the efficient purging of deleterious mutations in D. sinensis facilitated its survival and repeated demographic recovery. By contrast, in D. dyeriana, increased genetic drift and reduced selection efficacy, due to recent severe population bottlenecks and a likely preponderance of vegetative propagation, resulted in fixation of strongly deleterious mutations, reduced fitness, and continuous population decline, with likely detrimental consequences for the species' future viability and adaptive potential. Overall, our findings highlight the significant impact of demographic history on levels of accumulation and purging of putatively deleterious mutations that likely determine the long‐term survival and extinction risk of Tertiary relict trees.
Characteristics of breastfeeding newborns in the first month of life with in-utero selective serotonin reuptake inhibitor medication exposure: a scoping review protocol
Authors: Cantin Christina , Snelgrove-Clarke Erna , Gaudet Laura , Ross-White Amanda 
Published: October 2023

Objective:
The objective of this scoping review is to understand the extent and type of evidence in relation to the characteristics of breastfeeding newborns in the first month of life who have been exposed in-utero to selective serotonin reuptake inhibitor (SSRI) medications.

Introduction:
SSRIs are the most prescribed antidepressant medication in pregnancy. Up to 30% of newborns who are prenatally exposed to SSRIs demonstrate withdrawal signs. Poor neonatal adaptation syndrome represents a constellation of signs observed in these newborns. Little information has been studied regarding breastfeeding, as it relates to the impact of in-utero SSRI exposure on the newborn. Parents have many questions regarding the safety of taking medications during pregnancy and breastfeeding. It is important for health care providers to collate evidence-based information and facilitate shared-decision making. We aim to identify the approaches researchers have used to investigate in-utero SSRI exposure among breastfed newborns to determine knowledge gaps.

Inclusion criteria:
Primary peer-reviewed studies will be considered for inclusion according to the following criteria: newborns, 31 days of age or less, with in-utero SSRI exposure in any trimester of pregnancy, who were breastfed or received breast-milk feedings.

Methods:
MEDLINE, CINAHL, EMBASE, LactMed, the Maternity and Infant Care Database, and Clinical Trials databases will be searched. JBI methodology will be used. Abstracts will be assessed for eligibility and full texts will be retrieved if they meet the inclusion criteria. Two reviewers will independently extract the data from identified studies using a data extraction form and the results will be summarized descriptively and in tabular format.

Review registration:
Open Science Framework (osf.io/2bt39)
In vitro
potency of xeruborbactam in combination with multiple β-lactam antibiotics in comparison with other β-lactam/β-lactamase inhibitor (BLI) combinations against carbapenem-resistant and extended-spectrum β-lactamase-producing
Enterobacterales
Authors: Lomovskaya Olga , Castanheira Mariana , Lindley Jill , Rubio-Aparicio Debora , Nelson Kirk , Tsivkovski Ruslan , Sun Dongxu , Totrov Maxim , Loutit Jeffery , Dudley Michael 
Published: October 2023

ABSTRACT

Recently, several β-lactam (BL)/β-lactamase inhibitor (BLI) combinations have entered clinical testing or have been marketed for use, but limited direct comparative studies of their
in vitro
activity exist. Xeruborbactam (XER, also known as QPX7728), which is undergoing clinical development, is a cyclic boronate BLI with potent inhibitory activity against serine (serine β-lactamase) and metallo-β-lactamases (MBLs). The objectives of this study were (i) to compare the potency and spectrum of β-lactamase inhibition by various BLIs in biochemical assays using purified β-lactamases and in microbiological assays using the panel of laboratory strains expressing diverse serine and metallo-β-lactamases and (ii) to compare the
in vitro
potency of XER in combination with multiple β-lactam antibiotics to that of other BL/BLI combinations in head-to-head testing against recent isolates of carbapenem-resistant
Enterobacterales
(CRE). Minimal inhibitory concentrations (MICs) of XER combinations were tested with XER at fixed 4 or 8 µg/mL, and MIC testing was conducted in a blinded fashion using Clinical and Laboratory Standards Institute reference methods. Xeruborbactam and taniborbactam (TAN) were the only BLIs that inhibited clinically important MBLs. The spectrum of activity of xeruborbactam included several MBLs identified in
Enterobacterales,
e.g., and various IMP enzymes and NDM-9 that were not inhibited by taniborbactam. Xeruborbactam potency against the majority of purified β-lactamases was the highest in comparison with other BLIs. Meropenem-xeruborbactam (MEM-XER, fixed 8 µg/mL) was the most potent combination against MBL-negative CRE with MIC
90
values of 0.125 µg/mL. MEM-XER and cefepime-taniborbactam (FEP-TAN) were the only BL/BLIs with activity against MBL-producing CREs; with MEM-XER (MIC
90
of 1 µg/mL) being at least 16-fold more potent than FEP-TAN (MIC
90
of 16 µg/mL). MEM-XER MIC values were ≤8 µg/mL for >90% of CRE, including both MBL-negative and MBL-positive isolates, with FEP-TAN MIC of >8 µg/mL. Xeruborbactam also significantly enhanced potency of other β-lactam antibiotics, including cefepime, ceftolozane, ceftriaxone, aztreonam, piperacillin, and ertapenem, against clinical isolates of
Enterobacterales
that carried various class A, class C, and class D extended-spectrum β-lactamases and carbapenem-resistant
Enterobacterales
, including metallo-β-lactamase-producing isolates. These results strongly support further clinical development of xeruborbactam combinations.
Evaluation of
in vitro
antiviral activity of SARS-CoV-2 M
pro
inhibitor pomotrelvir and cross-resistance to nirmatrelvir resistance substitutions
Authors: Tong Xiao , Keung Walter , Arnold Lee D. , Stevens Laura J. , Pruijssers Andrea J. , Kook Seunghyi , Lopatin Uri , Denison Mark , Kwong Ann D. 
Published: October 2023

ABSTRACT

The unprecedented scale of the COVID-19 pandemic and the rapid evolution of SARS-CoV-2 variants underscore the need for broadly active inhibitors with a high barrier to resistance. The coronavirus main protease (M
pro
) is an essential cysteine protease required for viral polyprotein processing and is highly conserved across human coronaviruses. Pomotrelvir is a novel M
pro
inhibitor that has recently completed a phase 2 clinical trial. In this report, we demonstrated that pomotrelvir is a potent competitive inhibitor of SARS-CoV-2 M
pro
with high selectivity against human proteases. In the enzyme assay, pomotrelvir is also active against M
pro
proteins derived from human coronaviruses CoV-229E, CoV-OC43, CoV-HKU1, CoV-NL63, MERS, and SARS-CoV. In cell-based SARS-CoV-2 replicon and SARS-CoV-2 infection assays, pomotrelvir has shown potent inhibitory activity and is broadly active against SARS-CoV-2 clinical isolates including Omicron variants. Many resistance substitutions of the M
pro
inhibitor nirmatrelvir confer cross-resistance to pomotrelvir, consistent with the finding from our enzymatic analysis that pomotrelvir and nirmatrelvir compete for the same binding site. In a SARS-CoV-2 infection assay, pomotrelvir is additive when combined with remdesivir or molnupiravir, two nucleoside analogs targeting viral RNA synthesis. In conclusion, our results from the
in vitro
characterization of pomotrelvir antiviral activity support its further clinical development as an alternative COVID-19 therapeutic option.
The newest Oxford Nanopore R10.4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling
Authors: Zhang Tianyuan , Li Hanzhou , Ma Silin , Cao Jian , Liao Hao , Huang Qiaoyun , Chen Wenli 
Published: October 2023

ABSTRACT
The long-read amplicon provides a species-level solution for the community. With the improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) R10.4.1 has been substantially enhanced, with an average of approximately 99%. To evaluate its effectiveness on amplicons, three types of microbiomes were analyzed by 16S ribosomal RNA (hereinafter referred to as “16S”) amplicon sequencing using Novaseq, Pacbio sequel II, and Nanopore PromethION platforms (R9.4.1 and R10.4.1) in the current study. We showed the error rate, recall, precision, and bias index in the mock sample. The error rate of ONT R10.4.1 was greatly reduced, with a better recall in the case of the synthetic community. Meanwhile, in different types of environmental samples, ONT R10.4.1 analysis resulted in a composition similar to Pacbio data. We found that classification tools and databases influence ONT data. Based on these results, we conclude that the ONT R10.4.1 16S amplicon can also be used for application in environmental samples.

IMPORTANCE
The long-read amplicon supplies the community with a species-level solution. Due to the high error rate of nanopore sequencing early on, it has not been frequently used in 16S studies. Oxford Nanopore Technologies (ONT) introduced the R10.4.1 flowcell with Q20+ reagent to achieve more than 99% accuracy as sequencing technology advanced. However, there has been no published study on the performance of commercial PromethION sequencers with R10.4.1 flowcells on 16S sequencing or on the impact of accuracy improvement on taxonomy (R9.4.1 to R10.4.1) using 16S ONT data. In this study, three types of microbiomes were investigated by 16S ribosomal RNA (rRNA) amplicon sequencing using Novaseq, Pacbio sequel II, and Nanopore PromethION platforms (R9.4.1 and R10.4.1). In the mock sample, we displayed the error rate, recall, precision, and bias index. We observed that the error rate in ONT R10.4.1 is significantly lower, especially when deletions are involved. First and foremost, R10.4.1 and Pacific Bioscience platforms reveal a similar microbiome in environmental samples. This study shows that the R10.4.1 full-length 16S rRNA sequences allow for species identification of environmental microbiota.