bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of nasoalveolar molding on nasolabial aesthetics in patients with cleft lip and palate during pre‐adolescence: A systematic review and meta‐analysis of retrospective studies
Authors: Likitkulthanaporn Ard , Boonpratham Supatchai , Honglertnapakul Yanasinee , Saengfai Nuntinee Nanthavanich , Chaweewannakorn Chaiyapol , Jongkhum Nannapat , Peanchitlertkajorn Supakit 
Published: January 2024

AbstractThis study aims to analyze long‐term effects of nasoalveolar molding (NAM) as a part of cleft primary management protocols on nasolabial aesthetics for patients with non‐syndromic cleft lip and palate by conducting a systematic review and meta‐analysis. Six electronic databases and two journals were searched up to July 2023. Studies comparing nasolabial outcomes between NAM and non‐NAM protocols were selected for further analysis. Nasolabial aesthetics were the outcome of interest. Data extraction, methodological quality assessment, risk of bias assessment, meta‐analysis and subgroup analysis were performed. Seven retrospective cohort studies were selected for a qualitative review and four for a quantitative analysis. The risk of bias assessment was moderate for most studies. Only studies utilizing the Asher‐McDade rating (AMR) were included for meta‐analyses. The protocols with NAM exhibited a significantly lower AMR score for vermillion border than other protocols. AMR scores for nasal form and nasal symmetry from protocols with NAM were significantly lower than protocols without any pre‐surgical infant orthopaedics (PSIO) but not significantly different from protocols with other PSIO techniques. The AMR score for nasolabial profile from protocols with NAM was not significantly different from other protocols. However, subgroup analysis demonstrated that protocol combining NAM and primary rhinoplasty significantly lowered AMR scores for nasal form, nasal symmetry and nasolabial profile. For patients with unilateral cleft lip with or without palate (UCLP), this study found that a protocol combining NAM and primary rhinoplasty improved nasolabial outcomes while a protocol with NAM alone offered only limited benefits. For patients with BCLP, the available evidence remains inconclusive. Performing NAM in combination with primary rhinoplasty improves nasolabial aesthetics in patients with UCLP. PROSPERO (CRD4202128384).
IFT80 promotes early bone healing of tooth sockets through the activation of TAZ/RUNX2 pathway
Authors: Zhao Ziwei , Geng Ying , Ni Qiaoqi , Chen Yue , Cao Yanan , Lu Yahui , Wang Hua , Wang Ruixia , Sun Wen 
Published: January 2024

AbstractIntraflagellar transport (IFT) proteins have been reported to regulate cell growth and differentiation as the essential functional component of primary cilia. The effects of IFT80 on early bone healing of extraction sockets have not been well studied. To investigate whether deletion of Ift80 in alveolar bone‐derived mesenchymal stem cells (aBMSCs) affected socket bone healing, we generated a mouse model of specific knockout of Ift80 in Prx1 mesenchymal lineage cells (Prx1Cre;IFT80f/f). Our results demonstrated that deletion of IFT80 in Prx1 lineage cells decreased the trabecular bone volume, ALP‐positive osteoblastic activity, TRAP‐positive osteoclastic activity, and OSX‐/COL I‐/OCN‐positive areas in tooth extraction sockets of Prx1Cre; IFT80f/f mice compared with IFT80f/f littermates. Furthermore, aBMSCs from Prx1Cre; IFT80f/f mice showed significantly decreased osteogenic markers and downregulated migration and proliferation capacity. Importantly, the overexpression of TAZ recovered significantly the expressions of osteogenic markers and migration capacity of aBMSCs. Lastly, the local administration of lentivirus for TAZ enhanced the expression of RUNX2 and OSX and promoted early bone healing of extraction sockets from Prx1Cre; IFT80f/f mice. Thus, IFT80 promotes osteogenesis and early bone healing of tooth sockets through the activation of TAZ/RUNX2 pathway.
The “5Ts” screening tool: Enhancements and threshold values for effective TMD identification
Authors: Liu C. G. , Yap A. U. , Fu K. Y. , Lei J. 
Published: January 2024

AbstractObjectiveThis study aimed to enhance the quintessential “five temporomandibular disorder (TMD) symptoms” (5Ts) screener by incorporating frequency options and distinguishing between TMJ and muscle pain. The diagnostic accuracy along with cut‐off points for the effective identification of TMDs was also established.MethodsParticipants, aged ≥18 years, were recruited from a university‐based hospital. After completing surveys encompassing demographic data and the enhanced 5Ts (with frequency options [5Ts‐F] and differentiation of TMJ/muscle pain [6Ts‐F]), protocolized interviews and clinical examinations were performed following DC/TMD. The diagnostic accuracy and best cut‐off points were determined with the area under the receiver operating characteristic curves (AUCs).Results324 participants were recruited (mean age 30.0 ± 11.4 years). Among these, 86.4% had TMDs. 5Ts exhibited high diagnostic accuracy for detecting all TMDs (AUC = 0.92) with sensitivity/specificity values of 83.9%/88.6%. Both 5Ts‐F and 6Ts‐F had slightly better accuracy (AUCs = 0.95/0.96), comparable sensitivity, and superior specificity (97.7%) compared to 5Ts. The best cut‐off points were 1.5 for 5Ts and 2.5 for 5Ts‐F/6Ts‐F.ConclusionsAlthough all three TMD screeners presented high diagnostic accuracy, 5Ts‐F/6Ts‐F had notably improved specificity. 5Ts scores of >1.5 and 5Ts‐F/6Ts‐F scores of >2.5 are to be applied for screening the presence of TMDs.
Genetic analysis and molecular mapping of fruit‐bearing habit locus (Bh1) in eggplant (Solanum melongenaL.)
Authors: K. H. Sulochana , Saha Partha , Tomar Bhoopal Singh , Behera Tusar Kanti , Ghoshal Chandrika , Verma Veerendra Kumar , Verma Aakriti , S Gopala Krishnan , Saha Namita Das , Ray Soham , Singh Sanjay Kumar , Madhav Maganti Sheshu 
Published: January 2024

AbstractIn eggplant, two kinds of fruit‐bearing habits can be observed, that is, cluster and solitary. The fruits grown in cluster are small in size but contribute towards yield and productivity. In this investigation, we have studied the genetics of fruit‐bearing habit and identified the SSR markers linked to this trait. Pusa Safed Baingan 1 (cluster bearer) and Pusa Hara Baingan 1 (solitary bearer) having contrasting traits were crossed to generate F1(20 plants), F2(215 plants), B1(73 plants) and B2(64 plants). Genetic analysis of the trait was carried out using Chi‐square test. The segregation of plants in F2suggested recessive epistasis with ratio of 9:3:4 (solitary: mixed: cluster) indicating involvement of two genes for controlling the fruit‐bearing habit. The parental polymorphic 6 SSR markers were used for genotyping F2plants and co‐segregated with the fruit‐bearing habit (Bh1) locus in 1:2:1. A linkage map covering 63.86 cm distance was developed, the SSR marker emf11A03 was closest with a distance of 4.37 cm,emk03O04at 12.5 cm distance, andemf21O06at 14.5 cm. The physical positions of the SSR markersemb01J19andemf11A03flankingBh1gene were located at 4.77 mb position of contig Sme2.5_05721.1 and 5.18 Mb position of contig Sme2.5_00994.1, respectively. The findings in the present study will be helpful in marker‐assisted breeding to enhance yield in eggplant.
Evidence for the involvement of keratinocyte‐derived microvesicle particles in the photosensitivity associated with xeroderma pigmentosum type A deficiency
Authors: Christian Lea , Manjrekar Pranali , Henkels Karen M. , Rapp Christine M. , Annamraju Risha , Lohade Rushabh P. , Singh Shikshita , Carpenter M. Alexandra , Khan Saman , Kemp Michael G. , Chen Yanfang , Sahu Ravi P. , Travers Jeffrey B. 
Published: January 2024

AbstractPhotosensitivity can be due to numerous causes. The photosensitivity associated with deficiency of xeroderma pigmentosum type A (XPA) has been previously shown to be associated with excess levels of the lipid mediator platelet‐activating factor (PAF) generated by the keratinocyte. As PAF has been reported to trigger the production of subcellular microvesicle particles (MVP) due to the enzyme acid sphingomyelinase (aSMase), the goal of these studies was to discern if PAF and aSMase could serve as therapeutic targets for the XPA deficiency photosensitivity. HaCaT keratinocytes lacking XPA generated greater levels of MVP in comparison to control cells. Mice deficient in XPA also generated enhanced MVP levels in skin and in plasma in response to UV radiation. Use of a genetic strategy with mice deficient in both XPA and PAF receptors revealed that these mice generated less MVP release as well as decreased skin erythema and cytokine release compared to XPA knockout mice alone. Finally, the aSMase inhibitor imipramine blocked UV‐induced MVP release in HaCaT keratinocytes, as well as XPA knockout mice. These studies support the concept that the photosensitivity associated with XPA involves PAF‐ and aSMase‐mediated MVP release and provides a potential pharmacologic target in treating this form of photosensitivity.
Checklist for Habitual Physical Activity (CHaPA) for adults 75 years and older: tool development and content and face validation
Authors: Arita Kuniko , Ishibashi Yu , Ishibashi Hitomi 
Published: January 2024

AbstractBackgroundPhysical activity significantly contributes to older adults' physical and mental health, suggesting that physical activity could enhance healthy life expectancy. Despite its importance for adults aged 75 and older, activity levels among a large proportion of individuals aged 75 and older in Japan do not meet the recommended levels of physical activity, indicating a need for specific strategies tailored to this age group. This study aimed to develop a screening tool that measures daily activities promoting physical activity among older adults and assessed its content and face validity.MethodsIn Stage 1, we derived constructs pertinent to physical activity from previous literature and formulated an item list based on a prior qualitative study we undertook in Japan that evaluated daily behaviours facilitating physical activity among older adults. During Stage 2, we assessed the content and face validity of the list utilising the Nominal Group Technique (NGT), involving eight experts. The content validity was confirmed through two scoring evaluation rounds, while the face validity was verified through the NGT discussion, focusing on the comprehensibility and appropriateness of the tool.ResultsWe created a tool with 22 items consisting of three constructs. The NGT participants modified eight of these items for the final assessment, resulting in a finalised tool comprising 22 items that satisfied the adaptation criteria. The content validity of these items was affirmed by median adequacy (>5.0 points) and interquartile range (<1.0 points). The NGT discussion consensus also confirmed satisfactory face validity.ConclusionThe newly developed tool, Checklist for Habitual Physical Activity (CHaPA) for adults 75 years and older, is a valid screening tool to assess the daily behaviours that facilitate physical activity. This self‐administered instrument aims to assist older adults who need to start and maintain physical activity daily. Before its widespread public deployment, further investigation of the tool's validity and reliability is necessary.
Reconciling risk as threat and opportunity: The social construction of risk in boardrooms
Authors: Bryce Cormac , Ashby Simon , Ring Patrick 
Published: January 2024

AbstractBoard directing is a continuous process of risk analysis and control in response to the duality of risk as threat and opportunity. Judgments are made and remade to simultaneously reduce the potential for damaging threats (e.g., fraud, reputation damage), while exploiting opportunities (e.g., new product development, mergers and acquisitions). Adopting an institutional logics approach, we explore this process of risk analysis and control through the varied subject identities (e.g., directorial roles), risk management practices (the procedures and tools used to identify, assess, and control risk), and risk objects (the product of risk identification, assessment, and control, e.g., a risk matrix or register) of boards. We argue that the contingent interaction between these identities, practices, and objects inform the “risk logic” of a board, which may draw attention to the notion of risk as threat, risk as opportunity, or both threat and opportunity. Using the testimony of 30 executive and nonexecutive directors that represent 62 companies from a range of public, private, and third‐sector organizations, we contribute to the literature on the microfoundations of risk analysis in organizations by shining a light on how board directors understand, assess, control, and ultimately govern risk in organizations.
RH genotypes and red cell alloimmunization rates in chronically transfused patients with sickle cell disease: A multisite study in the USA
Authors: Israelyan Narek , Vege Sunitha , Friedman David F. , Zhang Zhe , Uter Stacey , Fasano Ross M. , Yee Marianne , Piccone Connie , Kelly Shannon , Hankins Jane S. , Zheng Yan , Westhoff Connie M. , Chou Stella T. 
Published: January 2024

AbstractBackgroundRed cell alloimmunization remains a challenge for individuals with sickle cell disease (SCD) and contributes to increased risk of hemolytic transfusion reactions and associated comorbidities. Despite prophylactic serological matching for ABO, Rh, and K, red cell alloimmunization persists, in part, due to a high frequency of variant RH alleles in patients with SCD and Black blood donors.Study Design and MethodsWe compared RH genotypes and rates of alloimmunization in 342 pediatric and young adult patients with SCD on chronic transfusion therapy exposed to >90,000 red cell units at five sites across the USA. Genotyping was performed with RHD and RHCE BeadChip arrays and targeted assays.ResultsPrevalence of overall and Rh‐specific alloimmunization varied among institutions, ranging from 5% to 41% (p = .0035) and 5%–33% (p = .0002), respectively. RH genotyping demonstrated that 33% RHD and 57% RHCE alleles were variant in this cohort. Patients with RHCE alleles encoding partial e antigens had higher rates of anti‐e identified than those encoding at least one conventional e antigen (p = .0007). There was no difference in anti‐D, anti‐C, or anti‐E formation among patients with predicted partial or altered antigen expression compared to those with conventional antigens, suggesting that variant Rh on donor cells may also stimulate alloimmunization to these antigens.DiscussionThese results highlight variability in alloimmunization rates and suggest that a molecular approach to Rh antigen matching may be necessary for optimal prevention of alloimmunization given the high prevalence of variant RH alleles among both patients and Black donors.
Plasma cell‐free DNA in canine lymphoma patients as a novel material for genotyping
Authors: Kambayashi Satoshi , Ono Nanae , Tone Tomofumi , Baba Kenji , Okuda Masaru 
Published: January 2024

AbstractCanine lymphoma is a disease with high morbidity and poor long‐term prognosis, despite a high response rate to chemotherapy. In this study, we focused on liquid biopsy, in which small amounts of substances from body fluids were analysed, to determine whether cell‐free DNA (cfDNA) in the plasma can be used as a biomarker for lymphoma in dogs. We found that 23 patients with lymphoma had significantly higher cfDNA concentrations than the 12 healthy dogs (median 2360 ng/mL versus 299 ng/mL, p < .0001). Polymerase chain reaction for antigen receptor rearrangement (PARR) was also employed using cfDNA from the lymphoma group to investigate whether cfDNA could be used for the detection of genetic clonality of lymphomas, as well as the genomic DNA (gDNA) extracted from an original lesion in each case. The correlation of the PARR results between cfDNA and gDNA was observed in 100% of B‐cell lymphomas (10/10), 77.8% of T‐cell lymphomas (7/9), and 100% of other types of lymphomas (4/4), respectively. These results indicate that plasma cfDNA levels are increasing in canine lymphoma patients, that cfDNA concentration can be a novel diagnostic tool, and that it can be used as a diagnostic tool for PARR.
Extensive red blood cell matching considering patient alloimmunization risk
Authors: Wemelsfelder Merel L. , van de Weem Ronald H. G. , Luken Jessie S. , de Haas Masja , Niessen René W. L. M. , van der Schoot C. Ellen , Hoogeveen Han , Oyebolu Folarin B. , den Hertog Dick , Janssen Mart P. 
Published: January 2024

AbstractBackground and ObjectivesRed blood cell (RBC) transfusions pose a risk of alloantibody development in patients. For patients with increased alloimmunization risk, extended preventive matching is advised, encompassing not only the ABO‐D blood groups but also the most clinically relevant minor antigens: C, c, E, e, K, Fya, Fyb, Jka, Jkb, S and s. This study incorporates patient‐specific data and the clinical consequences of mismatching into the allocation process.Materials and MethodsWe have redefined the MINimize Relative Alloimmunization Risks (MINRAR) model to include patient group preferences in selecting RBC units from a finite supply. A linear optimization approach was employed, considering both antigen immunogenicity and the clinical impact of mismatches for specific patient groups. We also explore the advantages of informing the blood bank about scheduled transfusions, allowing for a more strategic blood distribution. The model is evaluated using historical data from two Dutch hospitals, measuring shortages and minor antigen mismatches.ResultsThe updated model, emphasizing patient group‐specific considerations, achieves a similar number of mismatches as the original, yet shifts mismatches among patient groups and antigens, reducing expected alloimmunization consequences. Simultaneous matching for multiple hospitals at the distribution centre level, considering scheduled demands, led to a 30% decrease in mismatches and a 92% reduction in shortages.ConclusionThe reduction of expected alloimmunization consequences by incorporating patient group preferences demonstrates our strategy's effectiveness for patient health. Substantial reductions in mismatches and shortages with multi‐hospital collaboration highlights the importance of sharing information in the blood supply chain.
Virtual Learning Communication Behaviors In Natural Birth Support Communities
Authors: Widyaningrum Alifiah Ghaniyyu , Sjafri Hubeis Aida Vitayala , Sarwoprasodjo Sarwititi , Matindas Krishnarini 
Published: January 2024

Cesarean delivery rates are rising globally, presenting a growing concern, particularly in countries like Indonesia. Amidst this trend, the Cerita VBAC (Vaginal Birth After Caesar) on Telegram emerges as a crucial platform for pregnant women seeking insights and preparations for expected deliveries following Cesarean sections. This study, grounded in the constructivism paradigm, delves into the experiences of active speakers within the Cerita VBAC Community, aiming to understand the dynamics and impact of this virtual support network. Employing narrative ethnography methods, this research focuses on four prominent contributors within the Cerita VBAC Community. The constructivist paradigm guides the exploration of their experiences, emphasizing the role of narrative ethnography in unravelling the intricacies of this unique social context. Data analysis involves a manual modification process complemented by NVIVO 12 Plus, a comprehensive data processing application. The findings highlight that the learning delivery mechanism through Telegram aligns with Kolb's Experiential Learning model. Community members actively share concrete experiences, engage in reflective processes, formulate abstract concepts, and participate in experiential learning. Telegram serves as an information exchange medium and creates a positive and supportive environment, empowering pregnant women to navigate labor challenges.
Population pharmacokinetics and humanized dosage regimens matching the peak, area, trough, and range of amikacin plasma concentrations in immune-competent murine bloodstream and lung infection models
Authors: Jiao Yuanyuan , Yan Jun , Sutaria Dhruvitkumar S. , Lu Peggy , Vicchiarelli Michael , Reyna Zeferino , Ruiz-Delgado Juan , Burk Elizabeth , Moon Eugene , Shah Nirav R. , Spellberg Brad , Bonomo Robert A. , Drusano George L. , Louie Arnold , Luna Brian M. , Bulitta Jürgen B. 
Published: January 2024

ABSTRACT

Amikacin is an FDA-approved aminoglycoside antibiotic that is commonly used. However, validated dosage regimens that achieve clinically relevant exposure profiles in mice are lacking. We aimed to design and validate humanized dosage regimens for amikacin in immune-competent murine bloodstream and lung infection models of
Acinetobacter baumannii
. Plasma and lung epithelial lining fluid (ELF) concentrations after single subcutaneous doses of 1.37, 13.7, and 137 mg/kg of body weight were simultaneously modeled via population pharmacokinetics. Then, humanized amikacin dosage regimens in mice were designed and prospectively validated to match the peak, area, trough, and range of plasma concentration profiles in critically ill patients (clinical dose: 25–30 mg/kg of body weight). The pharmacokinetics of amikacin were linear, with a clearance of 9.93 mL/h in both infection models after a single dose. However, the volume of distribution differed between models, resulting in an elimination half-life of 48 min for the bloodstream and 36 min for the lung model. The drug exposure in ELF was 72.7% compared to that in plasma. After multiple q6h dosing, clearance decreased by ~80% from the first (7.35 mL/h) to the last two dosing intervals (~1.50 mL/h) in the bloodstream model. Likewise, clearance decreased by 41% from 7.44 to 4.39 mL/h in the lung model. The humanized dosage regimens were 117 mg/kg of body weight/day in mice [administered in four fractions 6 h apart (q6h): 61.9%, 18.6%, 11.3%, and 8.21% of total dose] for the bloodstream and 96.7 mg/kg of body weight/day (given q6h as 65.1%, 16.9%, 10.5%, and 7.41%) for the lung model. These validated humanized dosage regimens and population pharmacokinetic models support translational studies with clinically relevant amikacin exposure profiles.