bluuebunny/crossref_metadata_2025_split huggingface dataset:
PagHCF106 negatively regulates drought stress tolerance in poplar (Populus alba × Populus glandulosa) by modulating stomatal aperture
Authors: Liu Junke , Ye Muying , Fan Zhenghao , Wang Yanli , Chen Jiayu , Li Hongbing , Deng Xiping , Kim Ho Soo , Kwak Sang-Soo , Ke Qingbo 
Published: January 2025

Abstract
Modulation of stomatal development and movement is a promising approach for creating water-conserving plants. Here, we identified and characterized the PagHCF106 gene of poplar (Populus alba × Populus glandulosa). The PagHCF106 protein localized predominantly to the chloroplast, and the PagHCF106 gene exhibited tissue-specific expression pattern. Overexpression of PagHCF106 rescued the reduced stomatal aperture and increased drought resistance of the hcf106 Arabidopsis mutant. CRISPR/Cas9-mediated genome editing introduced mutations in the core region of the PagHCF106 promoter, which was required for its activity, as confirmed by the GUS staining assay. Mutation of the PagHCF106 promoter reduced stomatal opening and water loss in poplar. In addition, the genome-edited poplar lines accumulated high levels of hydrogen peroxide (H2O2) in guard cells. Taken together, these results suggest that PagHCF106 negatively regulates drought stress tolerance by modulating stomatal aperture, which might be associated with reactive oxygen species (ROS) production. We propose that modification of the PagHCF106 promoter might be an efficient strategy for enhancing drought stress tolerance in poplar.
Somatostatin-expressing neurons in the medial prefrontal cortex promote sevoflurane anesthesia in mice
Authors: Tang Aichen , Xu Mao , Chen Xizu , Liu Juan , Wang Jiamin , Wang Ying , Cai Shuang , Shu Yue , Zheng Danxu , Yu Tian , Wang Yuan , Luo Tianyuan , Yu Shouyang 
Published: January 2025

Background:
The medial prefrontal cortex plays a crucial role in regulating consciousness. However, the specific functions of its excitatory and inhibitory networks during anesthesia remain uncertain. Here we explored the hypothesis that somatostatin interneurons in the medial prefrontal cortex enhance the effects of sevoflurane anesthesia by increasing GABA transmission to pyramidal neurons.

Methods:
EEG was utilized to reflect the depth of anesthesia. Immunostaining and fiber photometry were employed to assess neuronal activities and GABA delivery. The regulation of neuronal activity was achieved by chemogenetics and optogenetics.

Results:
The expression of c-Fos was increased in somatostatin neurons of the medial prefrontal cortex during sevoflurane anesthesia (air versus sevoflurane: 26.4 ± 6.5 % versus 48 ± 6.2 %, P=0.0007, n=5 mice). Chemogenetic inhibition or activation of somatostatin neurons in the medial prefrontal cortex reduced (from 84 ± 24 s to 51 ± 18 s, P=0.008, n=7 mice) or prolonged (from 97 ± 31 s to 140 ± 30 s, P=0.006, n=7 mice) the sevoflurane anesthesia recovery time. Increased GABA input to pyramidal neurons in the medial prefrontal cortex precedes sevoflurane-induced loss of consciousness (ΔF/F: from 0.46 ± 0.57 % to 2.25 ± 1.42 %, P=0.031, n=10 mice). Activation of somatostatin neurons in the medial prefrontal cortex, leading to a significant reduction in calcium signals within local pyramidal neurons (ΔF/F: from -0.14 ± 0.52 % to -10.08 ± 4.44 %, P=0.002, n=10 mice), and GABA input on pyramidal neurons increased (ΔF/F: from -0.001 ± 0.001 % to 0.28 ± 0.03 %, P=0.002, n=7 mice) in a time-locked manner. Chemogenetic inhibition of pyramidal neurons prolonged the recovery from sevoflurane anesthesia in mice (from 101 ± 46 s to 136 ± 54 s, P=0.017, n=19 mice).

Conclusions:
Cortical somatostatin neurons may inhibit local pyramidal neurons by enhancing GABA transmission, which increases the effectiveness of sevoflurane anesthesia.
Can febrile neutropenia re-invent its self?
Authors: Georgala Aspasia , Klastersky Jean 
Published: January 2025

Purpose of review
Febrile neutropenia as a complication of cytotoxic chemotherapies, remains a major event in the medical journey of hematology and oncology patients. In this review, we are trying to review the new elements and highlights that are shaping febrile neutropenia in nowadays.

Recent findings
Introduction of risk-stratification, expanded use of granulocyte-colony stimulating factor and oral treatment for selected patients and rapid administration of antibiotics revolutionized the treatment of febrile neutropenia. Oral treatment with moxifloxacine or amoxicillin-clavulanate + ciprofloxacin has already been widely tested and is actually a standard of care for a meticulously selected group of patients managed as ambulatory patients. Intravenous treatment of febrile neutropenia is a major challenge for clinicians and microbiologists since the blast of the “silent pandemic” of antimicrobial resistance.

Summary
In this setting, strategies that reduce the chances of febrile neutropenia, misuse of antibiotics and enhance the rigorous control of infections may offer a chance to improve the management of febrile neutropenia and offer to our patients the chance to continue their antineoplastic treatment without perturbations.
Is there still a place for lymph node dissection for stage III melanoma since the approval of adjuvant therapy
Authors: Mouna Oumnia , Hanssens Charlotte , Meyers Michel , Langouo Mireille 
Published: January 2025

Purpose of review
This review aims to explore the evolving management strategies for stage III melanoma, focusing on the comparative effectiveness of traditional surgical approaches like complete lymph node dissection (CLND) versus modern adjuvant therapies. It also examines the latest evidence on the efficacy, risks, and complications of these strategies, emphasizing the role of shared decision-making between patients and clinicians.

Recent findings
Recent clinical trials and meta-analyses, including the MSLT-II and DeCOG-SLT studies, have demonstrated that CLND may not significantly improve survival outcomes in melanoma patients with sentinel lymph node biopsy (SLNB)-positive status. Instead, a shift towards observation combined with adjuvant therapies such as immune checkpoint inhibitors and targeted therapies (for BRAF-mutant melanoma) has been observed. These approaches have been associated with similar or improved recurrence-free survival rates and reduced treatment-related complications. However, challenges remain in establishing standardized protocols for adjuvant therapy use.

Summary
The management of stage III melanoma is rapidly transitioning from routine CLND towards a more individualized approach that incorporates active surveillance and adjuvant therapies based on tumor biology and patient-specific factors. Multidisciplinary discussions are essential to guide treatment decisions, and further research is required to develop clear, evidence-based protocols.
Neoadjuvant immunotherapy in melanoma: pathological response as a surrogate endpoint?
Authors: Meyers Michel , Mouna Oumnia , Langouo Mireille 
Published: January 2025

Purpose of review
This review evaluates by analyzing recent studies whether pathological complete response (pCR) can be used as a reliable surrogate marker for overall survival (OS) in melanoma treated with neoadjuvant immunotherapy.

Recent findings
Trials like Neo-Combi, Neo-Trio and COMBI-Neo show that pCR is crucial for long-term success in targeted therapy for melanoma, while studies like OpACIN-neo and SWOG S1801 demonstrate that immunotherapy can provide durable benefits even with partial responses. Findings from NADINA and the INMC analysis highlight that immunotherapy achieves higher pathologic response rates and improved survival outcomes, offering broader benefits compared to the pCR-dependent outcomes of targeted therapy.

Summary
pCR serves as a critical prognostic biomarker across cancer types, with strong validation in breast cancer where it predicts long-term survival, particularly in aggressive subtypes like triple-negative and HER2-positive breast cancer. In melanoma, pCR is gaining prominence as a surrogate marker in neoadjuvant therapies, with its predictive value varying between targeted treatments – where achieving pCR is essential – and immunotherapies, which provide durable survival benefits even with partial responses. Despite its potential, the role of pCR as a universally reliable surrogate endpoint in melanoma requires further validation through cancer-specific studies to ensure accurate treatment guidance and personalized care.
Real-world implementation of pain science education and barriers to use in private practice physiotherapy settings: an Australia-wide cross-sectional survey
Authors: Wilson Monique V. , Braithwaite Felicity A. , Arnold John B. , Stanton Tasha R. 
Published: January 2025

Abstract
Physiotherapists are critically positioned to integrate education into patient care, including pain science education (PSE) to enhance management and outcomes. Anecdotally, many physiotherapists report difficulty providing PSE in private practice settings. Here, we aimed to explore current PSE use, knowledge, and barriers to implementation. A nationwide online (Qualtrics) survey of PSE-trained Australian private practice physiotherapists assessed use, knowledge (revised Neurophysiology of Pain Questionnaire [rNPQ]), concept application, implementation barriers, and resource awareness/use/preferences, exploring associations with individual/clinic-level factors (clinical experience, PSE training, work location). A total of 278 physiotherapists (62.9% female, 39.4 [11.8] years, 15.2 [11.6] years of experience, 37.2% rural/remote) completed the survey. Pain science knowledge (rNPQ: mean 10.4 [2.2]/13) and perceived PSE competence was high, although 30% supported inaccurate PSE concepts. Pain science education training via professional development course was associated with higher knowledge relative to university training (multivariable; β = 1.337, P < 0.001). Physiotherapists reported providing PSE to 61% of patients, with patient-related barriers (expecting other treatments: 94%; previous negative/contradictory PSE experiences: 89%), clinic-level barriers (time constraints: 77%; insufficient billing schedules: 57%), and clinician-specific barriers (difficulties identifying/addressing patient maladaptive beliefs/behaviours: 53%; cultural/demographic translation challenges: 46%) reported. Fewer years of clinical experience was associated with heightened worry that providing PSE might go wrong (multivariable; β = −0.034, P = 0.010) and/or damage therapeutic relationships (multivariable; β = −0.049, P < 0.001). Physiotherapists were aware of over 100 PSE resources, with varying levels of perceived use/effectiveness, yet were largely unaware of educational strategies. Physiotherapists called for reduced complexity and greater ability to individualise PSE resources. Findings will guide improvements in PSE training/resources, to maximise physiotherapists' confidence and preparedness to effectively implement PSE.
Influence of lung function on macro- and micro-structural brain changes in mid- and late-life
Authors: Wang Jiao , Xu Weige , Dove Abigail , Salami Alireza , Yang Wenzhe , Ma Xiangyu , Bennett David A. , Xu Weili 
Published: January 2025

Introduction:
Lung function has been associated with cognitive decline and dementia, but the extent to which lung function impacts brain structural changes remains unclear. We aimed to investigate the association of lung function with structural macro- and micro-brain changes across mid- and late-life.

Methods:
The study included a total of 37 164 neurologic disorder-free participants aged 40–70 years from the UK Biobank, who underwent brain MRI scans 9 years after baseline. After 2.5 years, a subsample (n = 3895) underwent a second MRI scan. Lung function was assessed using a composite score based on forced expiratory volume in 1 second, forced vital capacity, and peak expiratory flow, and divided into tertiles (i.e., low, moderate, and high). Structural brain volumes (including total brain, gray matter, white matter, hippocampus, and white matter hyperintensities) and diffusion markers (fractional anisotropy [FA] and mean diffusivity [MD]) were assessed. Data were analyzed using linear regression and mixed-effects models.

Results:
Compared to high lung function, low lung function was associated with smaller total brain, gray matter, white matter, and hippocampal volume, as well as lower white matter integrity. Over the 2.5-year follow-up, low lung function was associated with reduced white matter and hippocampal volume, reduced FA, and increased white matter hyperintensity volume and MD. After stratification by age, the associations remained significant among adults aged 40–60 years and 60+ years.

Conclusion:
Low lung function is associated with macro- and micro-structural brain changes involving both neurodegenerative and vascular pathologies. This association is significant in both mid- and late-life.
Caring for Each Other
Authors: Gosselin Tracy , Pickett Lisa , Oliver John , Gross Michael 
Published: January 2025

Background:
Human errors, workplace violence, and community events can impact nurses and other health care workers’ (HCW) overall well-being. Peer support programs have shown a positive impact on care teams by focusing on second victim mitigation and support.

Purpose:
To determine the type of events and resource needs for a peer support program.

Methods:
This program evaluation piloted a 6-month peer support program and gathered prospective data on the volume, type, and length of debriefing events.

Results:
During the pilot, 192 referrals were made to the peer support program. This included 102 programmatic responses. These data supported the development of a business plan for ongoing program development.

Conclusions:
Peer support programs play a critical role in health care organizations by helping nurses and other HCWs manage the physical and emotional aspects of the work and the environment in which care is provided.
Implementation of an Evidence-Based Nursing Protocol for Mechanical Complications of Enteral Nutrition
Authors: Hu Min , Tu Ping , Xiong Fang-Ting , Ling Yan 
Published: January 2025

Background:
Patients admitted to the intensive care unit (ICU) are at risk for malnutrition. To provide sufficient nutritional support, enteral nutrition (EN) is the preferred method for patients admitted to the neurology ICU.

Purpose:
The purpose of this study was to examine the clinical effectiveness of an evidence-based nursing protocol for preventing and managing mechanical complications of EN for patients admitted to the neurology ICU.

Methods:
A randomized controlled trial design was used. The control group received general care, and the test group received care per the evidence-based nursing protocol.

Results:
A total of 118 patients participated in the study (59 each in the control and test group). The test group showed improved nutritional status measured by the Nutrition Risk Screening 2002 score, lower acute gastrointestinal injury grading, improved serum nutritional indicators, and less mechanical complications.

Conclusion:
Implementing an evidence-based nursing protocol was feasible and led to positive patient outcomes.
Taking a Pulse on Quality and Safety Education
Authors: Djukic Maja , Thompson Chantell , Ke Zhichun , Phan Jason , Padhye Nikhil 
Published: January 2025

Background:
The COVID-19 pandemic radically reshaped nurses’ education with a prominent shift to online learning. While there are emerging studies on quality and safety education post-pandemic, large-scale studies examining the pandemic’s impact on nurses’ quality and safety education are not available.

Purpose:
We examined quality and safety education in a national sample of nurses who were first licensed to practice in 2020, 2021, and 2022, adjusting for demographic, education, and work characteristics.

Methods:
We used correlational research design to analyze data from 821 nurses, which corresponded to a weighted population estimate of 170 824. Weighted logistic regression was used to model the nursing degree type on quality and safety education variables.

Results:
Approximately 90% of nurses reported receiving sufficient training in patient-centered care, teamwork and collaboration, and evidence-based practice. Approximately 75% received sufficient training in quality improvement.

Conclusions:
In a national survey conducted post COVID-19 pandemic, nurses reported a high level of preparedness in quality and safety education. One area that may require additional attention is quality improvement education.
A Chain of Events Leading to Posttraumatic Subacute Meningitis
Authors: Miyakawa-Liu Monica Harumi , Jorden Michelle A. , Aljinovic Nika 
Published: January 2025

Abstract
There are few reports that discuss the nebulous entity known as posttraumatic subacute meningitis. Herein, we describe a case where a male was found deceased with Streptococcus pyogenes meningitis 7 days after experiencing head trauma inflicted with a tow chain. Computed tomography scan prior to death revealed a scalp laceration with subcutaneous gas and a subdural hematoma. On autopsy, the lacerations had exposed the cranial bone, but no fracture was identified. Purulence and a pinpoint defect were observed on the dura. Histologic sections of the dura and brain showed a chronic inflammatory infiltrate, ventriculitis, and vascular involvement by bacteria. Toxicology studies were positive for methamphetamine, fentanyl, and oxycodone. Post-mortem cerebrospinal fluid and blood cultures grew S. pyogenes, complementing the gram-staining of the bacteria seen in the brain sections.
While the overlying parietal bone in the area of purulence was notably thinned, a direct route of infection from the soft tissue laceration to the meninges was not grossly identified. The middle ear mastoid cells did not display purulence, and the antemortem computed tomography scan did not provide any support for an otorhinolaryngeal source for the Streptococcus meningitis. To our knowledge, this is the first published report linking head trauma to S. pyogenes meningitis.
Role of Selective Neck Sutures in Facelifts
Authors: Ganesh Kumar Nishant , Sherif Rami , Schultz Kelly , Hidalgo David A. , Stuzin James M. , Rohrich Rod J. 
Published: January 2025

SUMMARY:
Several strategies have been proposed to prevent hematomas and improve cervical contour in facelifts. One such strategy is the use of selective neck sutures. This manuscript presents three surgical techniques to place selective sutures in facelift surgeries as an adjunct to intraoperative hemostasis and aid in neck contour. By promoting tissue adherence, these sutures aid in closing dead space, minimize serous collection, and aid in prompting early drain removal. The use of these sutures in addition to previously published strategies are effective means to minimize the risk of hematomas in facelifts and aid in improving cervical contour.
Immediate Hybrid Breast Reconstruction: Dual-plane approach using prepectoral implants and retro-pectoral fat grafting
Authors: Longo Benedetto , Giacalone Martina , D’Orsi Gennaro , Farcomeni Alessio , Gagliano Elettra , Vannucchi Lisa , Vanni Gianluca , Buonomo Claudio Oreste , Cervelli Valerio 
Published: January 2025

Background:
Implant-based breast reconstruction (BR) is to date the most popular reconstructive modality. The aim of the study was to evaluate the immediate hybrid one-stage BR technique, with a dual-plane approach, using prepectoral implants and retro-pectoral autologous fat transfer (AFT).

Methods:
We prospectively enrolled patients scheduled for immediate BR using a hybrid approach, which included retro-pectoral AFT and prepectoral breast implants (Group-A). This cohort was compared with a retrospective control group of patients who underwent immediate direct-to-implant BR without AFT (Group-B). Complications, hospitalization days, number of AFT procedures for a complete BR, aesthetic outcomes and patient’s satisfaction were analysed. Fat survival rate was assessed through comparison of preoperative and 6-months postoperative MRI.

Results:
30 immediate BR were included in each group. The average amount of AFT in the retro-pectoral plane was 106.30 cc (SD 16.54), while the mean breast implant size was statistically higher in group B (p=0.00026). MRI assessment confirmed an average of 47.90 % (SD 0.14) retro-pectoral fat survival at 6 postoperative months. No statistically significant differences in term of complications and hospitalization days were observed (p>0.05), while a significant difference was observed regarding additional AFT sessions (p=0.00062). After a propensity score weighted analysis, surgeons and patients assessment showed a significant higher overall satisfaction in the active group.

Conclusions:
Immediate hybrid approach for one-stage BR, enabled the use of smaller breast implants, a decrease in AFT procedures with costs reduction, and yielded optimal breast shape and contour, by reducing step-off deformity and rippling alterations, without adding complications.
Level of Evidence II
Dedifferentiated Fat Cells (DFATs) combined with Acellular Dermal Matrix to Promote the Survival of Grafted Fat——A Basic Research
Authors: Dong Miao , Ma Xuan , Li Facheng 
Published: January 2025

Background:
DFATs are derived from mature adipocytes that undergo dedifferentiation and regain proliferative and differentiation potential. Acellular Dermal Matrix (ADM) is used widely in plastic and reconstructive field. The effects of ADM combined with DFATs in fat transplantation has not been studied.

Methods:
In vitro, microstructure of ADM was tested. Adipogenic gene, and angiogenic factors secreted by ADSCs or DFATs seeded on ADM were measured. In vivo experiments involved pre-culturing DFATs on ADM and then mixing them with adipose tissue for transplantation into nude mice (1:2). Samples were collected at 4, 8, and 12 weeks for histological analysis, qRT-PCR, ELISA, and immunostaining.

Results:
ADM demonstrated good biocompatibility, and qRT-PCR showed that in vitro pre-culture enhanced the expression of adipogenic-related genes (PPARγ, CEBPα) in DFATs, and increased the secretion of VEGF (*P < 0.05). In the animal experiments, the fat grafts mixed with ADM exhibited better fat integrity, lower fibrosis levels and ELISA showed IL-1β、TNFα level was decreased. In DFATs+ADM group, the number of CD31+ cells and perilipin+ cells within the ADM were the largest (**P < 0.01). However, there was no significant difference in the expression of CD11b, iNOS/CD206 compared to the ADSCs+ADM group.

Conclusion:
ADM as a commercialized biomaterial enhances the paracrine function of seeded cells. When cultured on ADM and assisted fat transplantation, DFATs could better improve the angiogenesis and fat survival. The clinical application of 'cell-scaffold-fat transplantation' model in the future is promising.