bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determining Degree of Hypertrophy and Extent of Resection in Aesthetic Labia Minora Reduction: Technical Highlights and Step-by-Step Video Guide
Authors: Munhoz Alexandre Mendonça , Marques Neto Ary de Azevedo 
Published: November 2023

Summary:
Advances in aesthetic labia minora (LM) procedures have led to better results and fewer complications. Edge trim or wedge approaches are used, both of which use hidden incisions and preserve the edges of the LM. Since 1998, we have been performing a simple and reliable technique based on inferior wedge LM resection and superior pedicle flap reconstruction. Although details of aesthetic LM reduction have been amply published, previous technical videos have not presented a detailed step-by-step description, and few studies specifically address how to estimate the amount of tissue to resect according to the degree of hypertrophy. This article and the accompanying videos provide a detailed guide that clearly outlines the senior author’s (A.M.M.) technique for aesthetic LM reduction. The authors’ experience demonstrates that superior pedicle flap reconstruction is a reliable and reproducible technique. The success of the procedure depends on patient selection, careful preoperative planning, and adequate intraoperative management.
Primary HIV infection features colonic damage and neutrophil inflammation yet containment of microbial translocation
Authors: Tincati Camilla , Bono Valeria , Cannizzo Elvira Stefania , Tosi Delfina , Savi Federica , Falcinella Camilla , Casabianca Anna , Orlandi Chiara , Luigiano Carmelo , Augello Matteo , Rusconi Stefano , Muscatello Antonio , Bandera Alessandra , Calcagno Andrea , Gori Andrea , Nozza Silvia , Marchetti Giulia 
Published: November 2023

Introduction:
Impairment of the gastrointestinal barrier leads to microbial translocation and peripheral immune activation, which are linked to disease progression. Data in the setting of primary HIV/SIV infection suggest that gut barrier damage is one of the first events of the pathogenic cascade, preceding mucosal immune dysfunction and microbial translocation. We assessed gut structure and immunity as well as microbial translocation in acutely and chronically-infected, combination antiretroviral therapy (cART)-naive individuals.

Methods:
Fifteen people with primary HIV infection (P-HIV) and 13 with chronic HIV infection (C-HIV) c-ART-naive participants were cross-sectionally studied. Gut biopsies were analysed in terms of gut reservoirs (total, integrated and unintegrated HIV DNA); tight junction proteins (E-cadherin, Zonula Occludens-1), CD4+ expression, neutrophil myeloperoxidase (histochemical staining); collagen deposition (Masson staining). Flow cytometry was used to assess γδ T-cell frequency (CD3+panγδ+Vδ1+/Vδ2+). In plasma, we measured microbial translocation (LPS, sCD14, EndoCAb) and gut barrier function (I-FABP) markers (ELISA).

Results:
P-HIV displayed significantly higher tissue HIV DNA, yet neutrophil infiltration and collagen deposition in the gut were similar in the two groups. In contrast, microbial translocation markers were significantly lower in P-HIV compared with C-HIV. A trend to higher mucosal E-cadherin, and gut γδ T-cells was also observed in P-HIV.

Conclusion:
Early HIV infection features higher HIV DNA in the gut, yet comparable mucosal alterations to those observed in chronic infection. In contrast, microbial translocation is contained in primary HIV infection, likely because of a partial preservation of E-cadherin and mucosal immune subsets, namely γδ T-cells.
Computed Tomography–Based Radiomics Signature for Predicting Segmental Chromosomal Aberrations at 1p36 and 11q23 in Pediatric Neuroblastoma
Authors: Wang Haoru , Yu Chunlin , Ding Hao , Zhang Li , Chen Xin , He Ling 
Published: November 2023

ObjectiveThis study aimed to develop and assess the precision of a radiomics signature based on computed tomography imaging for predicting segmental chromosomal aberrations (SCAs) status at 1p36 and 11q23 in neuroblastoma.MethodsEighty-seven pediatric patients diagnosed with neuroblastoma and with confirmed genetic testing for SCAs status at 1p36 and 11q23 were enrolled and randomly stratified into a training set and a test set. Radiomics features were extracted from 3-phase computed tomography images and analyzed using various statistical methods. An optimal set of radiomics features was selected using a least absolute shrinkage and selection operator regression model to calculate the radiomics score for each patient. The radiomics signature was validated using receiver operating characteristic curves to obtain the area under the curve and 95% confidence interval (CI).ResultsEight radiomics features were carefully selected and used to compute the radiomics score, which demonstrated a statistically significant distinction between the SCAs and non-SCAs groups in both sets. The radiomics signature achieved an area under the curve of 0.869 (95% CI, 0.788–0.943) and 0.883 (95% CI, 0.753–0.978) in the training and test sets, respectively. The accuracy of the radiomics signature was 0.817 and 0.778 in the training and test sets, respectively. The Hosmer-Lemeshow test confirmed that the radiomics signature was well calibrated.ConclusionsComputed tomography–based radiomics signature has the potential to predict SCAs at 1p36 and 11q23 in neuroblastoma.
Myocardial, Retro-orbital, and Bilateral Testicular Metastases of an Ileal Neuroendocrine Tumor in 68Ga-DOTATATE PET/CT
Authors: Rogeau Antoine , Kaplar Zoltan , Fraioli Francesco 
Published: November 2023

Abstract
A 71-year-old man with a newly discovered metastatic grade II neuroendocrine tumor of the terminal ileum was referred for a 68Ga-DOTATATE PET/CT scan to stage the disease and assess suitability for PRRT (peptide receptor radionuclide therapy). The patient was known to have secondary nodal and bone/liver metastatic disease through prior morphological investigations. PET images revealed an atypical pattern of metastatic disease, showcasing secondary lesions in bilateral extraocular muscles, the myocardium, and both testes. The patient was pauci-symptomatic and only reported fatigue and diarrhea. Management involved lanreotide administration, and PRRT is being envisaged.
In Vivo Analysis of Porous Bioactive Silicon Carbide Scaffold for Craniofacial Bone Augmentation
Authors: Alfotawi Randa , Premnath Sangeetha , El-Ghannam Ahmad , Alsafadi Mona , Mahmood Amer 
Published: November 2023

Background:
Bone augmentation is a vital area of research because of its high clinical demand and the reported complications associated with the available biomaterials. Purpose: The study assess the role of decellurized skeletal muscle (DSM) when combined with synthesized porous bioactive silicon carbide (SiC) ceramic and evaluated its ability to augment bone calvaria in a rat model.

Material and Methods:
Eighteen rats were divided into 2 groups; group 1 (n=9), SiC discs (10 × 0.2 mm) pre-treated with 20% NaOH were placed as an onlay grafts on calvarial bone. Meanwhile, in group 2 (n=9), SiC discs pre-treated with 20% NaOH (10 × 0.2 mm) were covered with DSM. After 12 weeks, the grafted tissues were harvested and examined using cone-beam computed tomography, mechanical testing, and histologic analysis.

Results:
Cone-beam computed tomography for group 2 showed more radio-opacity for the remnant of SiC compared with native bone. The surface area and volume of radio-opacity were 2.48 mm2 ± 1.6 and 14.9 ± 7.8 mm3, respectively. The estimated quantitative average surface area of the radio-opacity for group 1 and volume were 2.55 mm2 ± (Sd=3.7) and 11.25 ± (Sd=8.9), respectively. Mechanically, comparable values of the flexural strength and statistically significant higher modulus of elasticity of calvaria in group 1 compared with group 2 and control (P<0.001). Histologically, group 2 region of woven bone was seen close to the lamellar bone (native bone), and there was immature bone present near the implanted SiC.

Conclusion:
The tested construct made of SiC/DSM has potential to osteointegrate into native bone, making it a suitable material for bone augmentation.
Wait Time Advantage for Transplant Candidates With HIV Who Accept Kidneys From Donors With HIV Under the HOPE Act
Authors: Motter Jennifer D. , Hussain Sarah , Brown Diane M. , Florman Sander , Rana Meenakshi M. , Friedman-Moraco Rachel , Gilbert Alexander J. , Stock Peter , Mehta Shikha , Mehta Sapna A. , Stosor Valentina , Elias Nahel , Pereira Marcus R. , Haidar Ghady , Malinis Maricar , Morris Michele I. , Hand Jonathan , Aslam Saima , Schaenman Joanna M. , Baddley John , Small Catherine B. , Wojciechowski David , Santos Carlos A.Q. , Blumberg Emily A. , Odim Jonah , Apewokin Senu K. , Giorgakis Emmanouil , Bowring Mary Grace , Werbel William A. , Desai Niraj M. , Tobian Aaron A.R. , Segev Dorry L. , Massie Allan B. , Durand Christine M. ,   on behalf of the HOPE in Action Investigators
Published: November 2023

Background.
Kidney transplant (KT) candidates with HIV face higher mortality on the waitlist compared with candidates without HIV. Because the HIV Organ Policy Equity (HOPE) Act has expanded the donor pool to allow donors with HIV (D+), it is crucial to understand whether this has impacted transplant rates for this population.

Methods.
Using a linkage between the HOPE in Action trial (NCT03500315) and Scientific Registry of Transplant Recipients, we identified 324 candidates listed for D+ kidneys (HOPE) compared with 46 025 candidates not listed for D+ kidneys (non-HOPE) at the same centers between April 26, 2018, and May 24, 2022. We characterized KT rate, KT type (D+, false-positive [FP; donor with false-positive HIV testing], D– [donor without HIV], living donor [LD]) and quantified the association between HOPE enrollment and KT rate using multivariable Cox regression with center-level clustering; HOPE was a time-varying exposure.

Results.
HOPE candidates were more likely male individuals (79% versus 62%), Black (73% versus 35%), and publicly insured (71% versus 52%; P < 0.001). Within 4.5 y, 70% of HOPE candidates received a KT (41% D+, 34% D–, 20% FP, 4% LD) versus 43% of non-HOPE candidates (74% D–, 26% LD). Conversely, 22% of HOPE candidates versus 39% of non-HOPE candidates died or were removed from the waitlist. Median KT wait time was 10.3 mo for HOPE versus 60.8 mo for non-HOPE candidates (P < 0.001). After adjustment, HOPE candidates had a 3.30-fold higher KT rate (adjusted hazard ratio = 3.30, 95% confidence interval, 2.14-5.10; P < 0.001).

Conclusions.
Listing for D+ kidneys within HOPE trials was associated with a higher KT rate and shorter wait time, supporting the expansion of this practice for candidates with HIV.
SARS-CoV-2 Variants Omicron BA.4/5 and XBB.1.5 Significantly Escape T Cell Recognition in Solid-organ Transplant Recipients Vaccinated Against the Ancestral Strain
Authors: Halvorson Torin , Ivison Sabine , Huang Qing , Ladua Gale , Yotis Demitra M. , Mannar Dhiraj , Subramaniam Sriram , Ferreira Victor H. , Kumar Deepali , Belga Sara , Levings Megan K. ,   on behalf of the PREVenT Study Group
Published: November 2023

Background.
Immune-suppressed solid-organ transplant recipients (SOTRs) display impaired humoral responses to COVID-19 vaccination, but T cell responses are incompletely understood. SARS-CoV-2 variants Omicron BA.4/5 (BA.4/5) and XBB.1.5 escape neutralization by antibodies induced by vaccination or infection with earlier strains, but T cell recognition of these lineages in SOTRs is unclear.

Methods.
We characterized Spike-specific T cell responses to ancestral SARS-CoV-2 and BA.4/5 peptides in 42 kidney, liver, and lung transplant recipients throughout a 3- or 4-dose ancestral Spike mRNA vaccination schedule. As the XBB.1.5 variant emerged during the study, we tested vaccine-induced T cell responses in 10 additional participants using recombinant XBB.1.5 Spike protein. Using an optimized activation-induced marker assay, we quantified circulating Spike-specific CD4+ and CD8+ T cells based on antigen-stimulated expression of CD134, CD69, CD25, CD137, and/or CD107a.

Results.
Vaccination strongly induced SARS-CoV-2-specific T cells, including BA.4/5- and XBB.1.5-reactive T cells, which remained detectable over time and further increased following a fourth dose. However, responses to BA.4/5 (1.34- to 1.67-fold lower) XBB.1.5 (2.0- to 18-fold lower) were significantly reduced in magnitude compared with ancestral strain responses. CD4+ responses correlated with anti-receptor-binding domain antibodies and predicted subsequent antibody responses in seronegative individuals. Lung transplant recipients receiving prednisone and older adults displayed weaker responses.

Conclusions.
Ancestral strain vaccination stimulates BA.4/5 and XBB.1.5-cross-reactive T cells in SOTRs, but at lower magnitudes. Antigen-specific T cells can predict future antibody responses. Our data support monitoring both humoral and cellular immunity in SOTRs to track COVID-19 vaccine immunogenicity against emerging variants.
Empedobacter sedimenti sp. nov., isolated from sediment of East Taihu Lake
Authors: Ruan Luyao , Liu Le , Jiang Xueting , Mao Dongmei , Shao Jiahui , He Jian , Shen Qirong 
Published: November 2023

A Gram-reaction-negative, strictly aerobic, pale yellow, non-gliding, rod-shaped bacterium, designated DT-LB-19T, was isolated from the sediment of East Taihu Lake in Jiangsu Province, PR China. Strain DT-LB-19T showed the highest 16S rRNA gene sequence similarities to members of the genera

Algoriella

,

Chishuiella

and

Empedobacter

(94.84–95.77 %) in the family

Weeksellaceae

. In phylogenetic trees based on genomes, strain DT-LB-19T clustered within the genus

Empedobacter

but formed a separate subclade with a high bootstrap value. The average nucleotide identity and digital DNA–DNA hybridization values between DT-LB-19T and the closely related type strains were in the range of 82.5–86.9 % and 25.8–32.3 %, respectively. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 1
ω5c, C16 : 0, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 03-OH. The predominant menaquinone was menaquinone-6. The polar lipid profile consisted of phosphatidylethanolamine, one glycolipid, two aminophospholipids and five unidentified lipids. The DNA G+C content was 31.8 mol%. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic results, we propose that strain DT-LB-19T represents a novel species of the genus

Empedobacter

, for which the name Empedobacter sedimenti sp. nov. is proposed, with strain DT-LB-19T (=KCTC 82330T=CCTCC AB 2023026T= JSACC 11448T) as the type strain.
Protection of β cells against pro-inflammatory cytokine stress by the GDF15-ERBB2 signaling
Authors: Sarkar Soumyadeep , Syed Farooq , Webb-Robertson Bobbie-Jo , Melchior John T. , Chang Garrick , Gritsenko Marina , Wang Yi-Ting , Tsai Chia-Feng , Liu Jing , Yi Xiaoyan , Cui Yi , Eizirik Decio L. , Metz Thomas O. , Rewers Marian , Evans-Molina Carmella , Mirmira Raghavendra G. , Nakayasu Ernesto S. 
Published: November 2023

ABSTRACTAim/hypothesisGrowth/differentiation factor 15 (GDF15) is a therapeutic target for a variety of metabolic diseases, including type 1 diabetes (T1D). However, the nausea caused by GDF15 is a challenging point for therapeutic development. In addition, it is unknown why the endogenous GDF15 fails to protect from T1D development. Here, we investigate the GDF15 signaling in pancreatic islets towards opening possibilities for therapeutic targeting in β cells and to understand why this protection fails to occur naturally.MethodsGDF15 signaling in islets was determined by proximity-ligation assay, untargeted proteomics, pathway analysis, and treatment of cells with specific inhibitors. To determine if GDF15 levels would increase prior to disease onset, plasma levels of GDF15 were measured in a longitudinal prospective study of children during T1D development (n=132 cases vs. n=40 controls) and in children with islet autoimmunity but normoglycemia (n=47 cases vs. n=40 controls) using targeted mass spectrometry. We also investigated the regulation of GDF15 production in islets by fluorescence microscopy and western blot analysis.ResultsThe proximity-ligation assay identified ERBB2 as the GDF15 receptor in islets, which was confirmed using its specific antagonist, tucatinib. The untargeted proteomics analysis and caspase assay showed that ERBB2 activation by GDF15 reduces β cell apoptosis by downregulating caspase 8. In plasma, GDF15 levels were higher (p=0.0024) during T1D development compared to controls, but not in islet autoimmunity with normoglycemia. However, in the pancreatic islets GDF15 was depleted via sequestration of its mRNA into stress granules, resulting in translation halting.Conclusions/interpretationGDF15 protects against T1D via ERBB2-mediated decrease of caspase 8 expression in pancreatic islets. Circulating levels of GDF15 increases pre-T1D onset, which is insufficient to promote protection due to its localized depletion in the islets. These findings open opportunities for targeting GDF15 downstream signaling for pancreatic β cell protection in T1D and help to explain the lack of natural protection by the endogenous protein.
Computation on demand: Action-specific representations of visual task features arise during distinct movement phases
Authors: Lee Nina , Guo Lin Lawrence , Nestor Adrian , Niemeier Matthias 
Published: November 2023

AbstractIt is commonly held that computations of goal-directed behaviour are governed by conjunctive neural representations of the task features. However, support for this view comes from paradigms with arbitrary combinations of task features and task affordances that require representations in working memory. Therefore, in the present study we used a task that is well-rehearsed with task features that afford minimal working memory representations to investigate the temporal evolution of feature representations and their potential integration in the brain. Specifically, we recorded electroencephalography data from human participants while they first viewed and then grasped objects or touched them with a knuckle. Objects had different shapes and were made of heavy or light materials with shape and weight being features relevant for grasping but not for knuckling. Using multivariate analysis, we found that representations of object shape were similar for grasping and knuckling. However, only for grasping did early shape representations reactivate at later phases of grasp planning, suggesting that sensorimotor control signals feed back to early visual cortex. Grasp-specific representations of material/weight only arose during grasp execution after object contact during the load phase. A trend for integrated representations of shape and material also became grasp-specific but only briefly during movement onset. These results argue against the view that goal-directed actions inevitably join all features of a task into a sustained and unified neural representation. Instead, our results suggest that the brain generates action-specific representations of relevant features as required for the different subcomponent of its action computations.Significance statementThe idea that all the features of a task are integrated into a joint representation or event file is widely supported but importantly based on paradigms with arbitrary stimulus-response combinations. Our study is the first to investigate grasping using electroencephalography to search for the neural basis of feature integration in such a daily-life task with overlearned stimulus-response mappings. Contrary to the notion of event files we find limited evidence for integrated representations. Instead, we find that task-relevant features form representations at specific phases of the action. Our results show that integrated representations do not occur universally for any kind of goal-directed behaviour but in a manner of computation on demand.
AKAP8 interacting with DDX5 to regulate R-loop balance involves in lung carcinoma cell growth
Authors: Wang Xu , Liu Liang 
Published: November 2023

AbstractTriple-nucleotide chain of RNA:DNA hybrid structure R-loop is formed during transcription process which is closely associated with transcriptional regulation, transcriptional termination, epigenetic modifications, and structure of chromatin. Dysregulation of R-loop formation or resolution may disturb normal DNA replication or RNA transcription, leading to associated progress of disease. Herein, we found A-kinase-anchoring protein 8 (AKAP8) bind with R-loop structure in cell. Knock down of AKAP8 showed perturbation of balance of genomic R-loop formation and gene transcription. Evidences was shown that AKAP8 interacted with R-loop resolution protein ATP-dependent DEAD box RNA helicase DDX5 and increased chromatin associated DDX5 level. Finally, AKAP8 promoted UCP2 transcription and resolved R-loop level of its promoter region, and contributed to cell growth of adenocarcinomic human alveolar basal epithelial cell line A549, which may provide clues for poor prognosis of lung adenocarcinoma in high AKAP8 expression patients.
Identifying the attributes of consumer experience in Michelin-starred restaurants: a text-mining analysis of online customer reviews
Authors: Barrera-Barrera Ramón 
Published: November 2023

PurposeThe main goal of this paper is to identify the attributes of consumer experience in Michelin-starred restaurants and to estimate their effects on restaurant ratings.Design/methodology/approachA sample of 70,233 online reviews of 224 Spanish Michelin-starred restaurants were analysed with the latent Dirichlet allocation algorithm. A sentiment analysis and a logistic regression analysis were also employed to estimate the effect of attributes on restaurant ratings.FindingsCustomer attention, food quality, decor and ambience and value for money are frequently used to define restaurant experience. However, it is shown in this study that the experience in a Michelin-starred restaurant goes beyond the evaluation of those four attributes. Furthermore, the effect of the factors that were identified on customer satisfaction differed depending on the restaurant ratings.Research limitations/implicationsThe findings are linked to the context of Spanish Michelin-starred restaurants. It is also assumed in this study that online reviews are based on truthful opinions.Practical implicationsRestaurant managers should primarily focus on customer attention and food quality to achieve customer satisfaction. In addition, those restaurants with an error-free service and a highly appreciated wine list among diners are more likely to achieve the culinary excellence that deserves a 5-star rating on TripAdvisor.Originality/valueThe attributes of the restaurant experience are frequently identified in literature reviews. Research based on text-mining analyses of customer reviews to discover a posteriori the factors that define a restaurant experience is scarce, and particularly difficult to find in the context of Michelin-starred restaurants.