bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED: The role of PKP1 in tumor progression in melanoma: Analysis of a cell adhesion‐related model
Authors: Yue Chao , Lian Wenqin , Fan Zhongru , Li Haochen , Duan Mengying , Qin Lingshan , Cao Xianbin , Peng Jianzhong 
Published: November 2023

AbstractThe incidence rate of melanoma varies across regions, with Europe, the United States, and Australia having 10–25, 20–30, and 50–60 cases per 1 00 000 people. In China, patients with melanoma exhibit different clinical manifestations, pathogenesis, and outcomes. Current treatments include surgery, adjuvant therapy, and immune checkpoint inhibitors. Nonetheless, complications may arise during treatment. Melanoma development is heavily reliant on cell adhesion molecules (CAMs), and studying these molecules could provide new research directions for metastasis and progression. CAMs include the integrin, immunoglobulin, selectin, and cadherin families, and they affect multiple processes, such as maintenance, morphogenesis, and migration of adherens junction. In this study, a cell adhesion‐related risk prognostic signature was constructed using bioinformatics methods, and survival analysis was performed. Plakophilin 1 (PKP1) was observed to be crucial to the immune microenvironment and has significant effects on melanoma cell proliferation, migration, invasion, and the cell cycle. This signature demonstrates high reliability and has potential for clinical applications.
Ecotoxic effect of agriculture and environmental important bioinoculant effective microorganism (EM) derived metabolites on microbial agro active agents
Authors: Namasivayam S. Karthick Raja , Lavanya M. , Sreesha  , Shyamsundar D. 
Published: November 2023

AbstractEffective microorganism (EM) – a mixture of beneficial microbial formulation are extensively utilized worldwide in agriculture as plant growth promotor and biocontrol agent. Because of their wide spectrum usage, it is necessary to study the non‐target effect against the beneficial organisms used in crop production. Though, the wider applications of EM in agriculture and the environment have revealed, the environmental quality management of EM mainly metabolites‐ biochemical compounds derived from EM have yet to be studied. With this objective, the present study is undertaken to evaluate the compatibility of EM derived metabolites on the selected microbial biofertilizers, biocontrol agents including biopesticidal agents. Metabolites were extracted from the culture‐free broth of EM culture using various solvents and the extracted metabolites were characterized by the gas chromatography‐ mass spectroscopy (GC‐MS). The food poisoning technique was adapted for the screening of toxic effects. Toxic effect of the metabolites derived from the respective solvent was confirmed by measuring the growth of the respective microbial strain's vegetative growth and sporulation efficacy by dual plate assay, dual liquid assay, and spore germination microscopic slide assay. Active principles of the metabolites were readily extracted in all the solvents and the possible presence of bioactive metabolites were characterized by GC‐MS. There was no significant difference between the control and extract recorded. In the case of Rhizobium leguminosarum, the colony diameters of ethyl acetate and acetone extract were 1.6 and 1.8 cm, respectively, as in control (1.8 cm). Similar findings were also recorded for Azospirillum lipoferum, Phosphobacterium sp., and Pseudomonas flurescens. However, growth inhibition including spore germination was recorded in petroleum ether and acetone extract. Further study will be useful to exploit the principles of EM and EM derived metabolites in agriculture and environment without affecting non target beneficial organism.
Statistical modeling of fully nonlinear hydrodynamic loads on offshore wind turbine monopile foundations using wave episodes and targeted CFD simulations through active sampling
Authors: Guth Stephen , Katsidoniotaki Eirini , Sapsis Themistoklis P. 
Published: November 2023

AbstractAccurately determining hydrodynamic force statistics is crucial for designing offshore engineering structures, including offshore wind turbine foundations, due to the significant impact of nonlinear wave–structure interactions. However, obtaining precise load statistics often involves computationally intensive simulations. Furthermore, the estimation of statistics using current practices is subject to ongoing discussion due to the inherent uncertainty involved. To address these challenges, we present a novel machine learning framework that leverages data‐driven surrogate modeling to predict hydrodynamic loads on monopile foundations while reducing reliance on costly simulations and facilitate the load statistics reconstruction. The primary advantage of our approach is the significant reduction in evaluation time compared to traditional modeling methods. The novelty of our framework lies in its efficient construction of the surrogate model, utilizing the Gaussian process regression machine learning technique and a Bayesian active learning method to sequentially sample wave episodes that contribute to accurate predictions of extreme hydrodynamic forces. Additionally, a spectrum transfer technique combines computational fluid dynamics (CFD) results from both quiescent and extreme waves, further reducing data requirements. This study focuses on reducing the dimensionality of stochastic irregular wave episodes and their associated hydrodynamic force time series. Although the dimensionality reduction is linear, Gaussian process regression successfully captures high‐order correlations. Furthermore, our framework incorporates built‐in uncertainty quantification capabilities, facilitating efficient parameter sampling using traditional CFD tools. This paper provides comprehensive implementation details and demonstrates the effectiveness of our approach in delivering reliable statistics for hydrodynamic loads while overcoming the computational cost constraints associated with classical modeling methods.
The Challenge
Authors: Braunerhjelm Pontus , Henrekson Magnus 
Published: November 2023

AbstractDuring recent years, challenges have emerged on an unprecedented scale, affecting nearly all the countries of the globe: climate change, energy supply, geopolitical tensions, protectionist measures, demographic changes, and a seemingly infinite demand for human capital, to mention but a few. Until recently, the most compelling challenge seemed to be increased globalization and intensified competition at all stages of the value chain. This threatened the privileged position of industrialized economies and their businesses, prompting measures to enhance efficiency and competitiveness at both the policy and firm levels. Even though the world economy currently seems to be in a phase of decoupling, deglobalization, and increased state intervention, we believe that in the medium to long term, the global economy will continue to embrace openness. Hence, fortune favors the well-prepared, and both countries and businesses are likely to benefit by positioning themselves for a return to the prospect of increasingly competitive markets.
Contactless single point incremental forming: experimental and numerical simulation
Authors: Almadani Mohammad , Guner Ahmet , Hassanin Hany , De Lisi Michele , Essa Khamis 
Published: November 2023

AbstractThe demand for small-batch manufacturing processes has increased considerably in recent years due to the need for personalized and customized products. Single point incremental forming (SPIF) has emerged as a time-efficient approach that offers increased material formability when compared to conventional sheet metal forming techniques. However, the complexity of SPIF requires a complete understanding of the material deformation mechanism. In this study, a non-conventional contactless tool in the form of hot compressed air is employed to form a polycarbonate sheet. The influence of the contactless tool on the shaping process is modeled and analyzed with a finite element modelling (FEM). Two different models were developed and coupled to estimate the resulting shape of the sheet. A CFD model was created to obtain pressure and temperature values of the air impacting the sheet, while a transient structural model was employed to study the deformation of the sheet. The research provides a working model that is able to predict the performance of this contactless incremental forming process of polymers with high accuracy. The comprehensive FE model developed in this work is able to forecast the final part geometries and dimensions in addition to the normal strain progression. It also revealed that the primary modes of deformation in SPIF were stretching, thinning, and bending. The model was validated by experimental results, and the predicted sheet deformation was compared to the one generated experimentally, and the results obtained were in good agreement.
Effect of dietary supplementation with Lactobacillus helveticus R0052 on seizure thresholds and antiseizure potency of sodium valproate in mice
Authors: Wlaź Piotr , Wiater Adrian , Majewska Małgorzata , Wyska Elżbieta , Grąz Marcin , Śliwa-Dominiak Joanna , Gapińska Nikola , Socała Katarzyna 
Published: November 2023

Abstract
Objective
Both animal and human studies, though limited, showed that multi-strain probiotic supplementation may reduce the number of seizures and/or seizure severity. Here, we evaluated the effect of a single strain probiotic supplementation on seizure susceptibility, antiseizure efficacy of sodium valproate, and several behavioral parameters in mice.

Methods
Lactobacillus helveticus R0052 was given orally for 28 days. Its influence on seizure thresholds was evaluated in the ivPTZ- and electrically-induced seizure tests. The effect on the antiseizure potency of valproate was assessed in the scPTZ test. We also investigated the effects of probiotic supplementation on anxiety-related behavior (in the elevated plus maze and light/dark box tests), motor coordination (in the accelerating rotarod test), neuromuscular strength (in the grip-strength test), and spontaneous locomotor activity. Serum and brain concentrations of valproate as well as cecal contents of SCFAs and lactate were determined using HPLC method.

Results
L. helveticus R0052 significantly increased the threshold for the 6 Hz-induced psychomotor seizure. There was also a slight increase in the threshold for myoclonic and clonic seizure in the ivPTZ test. L. helveticus R0052 did not affect the threshold for tonic seizures both in the maximal electroshock- and ivPTZ-induced seizure tests. No changes in the antiseizure potency of valproate against the PTZ-induced seizures were reported. Interestingly, L. helveticus R0052 increased valproate concentration in serum, but not in the brain. Moreover, L. helveticus R0052 did not produce any significant effects on anxiety-related behavior, motor coordination, neuromuscular strength, and locomotor activity. L. helveticus R0052 supplementation resulted in increased concentrations of total SCFAs, acetate, and butyrate.

Conclusions
Altogether, this study shows that a single-strain probiotic – L. helveticus R0052 may decrease seizure susceptibility and this effect can be mediated, at least in part, by increased production of SCFAs. In addition, L. helveticus R0052 may affect bioavailability of valproate, which warrants further investigations.
Clinical application of artificial intelligence-assisted three-dimensional planning in direct anterior approach hip arthroplasty
Authors: Yang Weihua , Gao Tianyi , Liu Xingyu , Shen Kaiwei , Lin Feitai , Weng Yan , Lin Bei , Liang Deng , Feng Eryou , Zhang Yiling 
Published: November 2023

Abstract
Purpose
The objective of this study was to investigate the efficacy of an artificial intelligence-assisted 3D planning system (AIHIP) in total hip arthroplasty by direct anterior approach and assess the reliability of the AIHIP preoperative program in terms of both interobserver and intraobserver agreement.

Methods
A retrospective analysis was conducted on patients who underwent unilateral primary THA via direct anterior approach from June 2019 to March 2022. Participants were randomly assigned to receive either the AIHIP system (n = 220) or the 2D template (control group) (n = 220) for preoperative planning. The primary outcome aimed to evaluate the correspondence between the prosthesis selected intro-operation and the one planned preoperatively, as well as to calculate the intraclass correlation coefficient (ICC). Secondary outcomes included operation time, intraoperative blood loss, fluoroscopy times, Harris hip score (HHS), lower limb length difference (LLD), femoral offset (FO), and bilateral femoral offset difference.

Results
No significant differences were observed in gender, age, body mass index (BMI), aetiology, and American Society of Anesthesiologists (ASA) score between the two groups. Both planning methods exhibited good intraobserver agreement for component planning (ICC: 0.941–0.976). Interobserver agreement for component planning was comparable between the two methods (ICC: 0.882–0.929). In the AIHIP group, the accuracy of acetabular cup and femoral stem prosthetics planning significantly improved, with accuracies within the size range of ± 0 and ± 1 being 76.8% and 90.5% and 79.5% and 95.5%, respectively. All differences between two groups were statistically significant (p < 0.05). Patients receiving AIHIP preoperative planning experienced shorter operation times, reduced intraoperative blood loss, fewer fluoroscopy times, and lower leg length discrepancy (LLD) (p < 0.05). Moreover, they demonstrated a higher Harris hip score (HHS) at three days post-surgery (p < 0.05). However, no significant differences were found in femoral offset (FO), difference of bilateral femoral offsets, and HHS at 1 month after the operation.

Conclusion
Utilizing AIHIP for preoperative planning of direct anterior approach THA can significantly enhance the accuracy of prosthetic sizing with good reliability, decrease operation time, reduce intraoperative blood loss, and more effectively restore the length of both lower limbs. This approach has greater clinical application value.
Cooperative parental performance at chick provisioning in a small passerine, the Reed Warbler Acrocephalus scirpaceus
Authors: Wojczulanis-Jakubas Katarzyna , Płóciennik Justyna , Guinebretiere Aldric , Hałupka Lucyna 
Published: November 2023

Abstract
Sexual conflict has long been perceived as a solid background for the evolution of parental care. Recent studies, however, highlight the importance of cooperation between the parents, especially in socially monogamous systems. Here, we examined parental performance of a small monogamous passerine with bi-parental care, the Eurasian Reed Warbler (Acrocephalus scirpaceus, RW), looking at the issue from the perspective of parents’ cooperation. Using accurate and non-invasive video data on parent chick provisioning from 78 nests varying in brood size, we found some evidence of parents cooperation: both parents similarly adjusted their feeding rate to brood size, and the higher was their both contribution, the better was chicks condition (expressed by defecation rate). However, contrary to our expectations (based on premises from similar studies on other bird species) we did not find evidence for frequent and active synchronisation of nest visits. Importantly, we found a negative relationship between synchronisation level and chick body condition, suggesting that synchronisation may actually not be that beneficial in the study system. The results are surprising and highlight the importance of studying various species to understand mechanisms behind cooperation of partners in the bi-parental care system.

Significance statement
Biparental care has long been viewed in the context of conflict between the breeding partners, and only recently they are considered cooperating agents caring together for the common offspring. Nevertheless, studies applying such a perspective are still scarce and there is a pressing need to test different species. Using video data on chick provisioning by Reed Warbler parents, we found evidence of partner cooperation: both parents adjusted their feeding rate to the number of nestlings, and the higher was their joint contribution, the better was offspring condition. Parents did not synchronise their nest visits more compared to randomly generated visits, but we found that synchronisation level negatively affected chick condition. Our results highlight the need to consider various species to fully understand mechanisms behind cooperation of breeding partners.
Time allocation to balance group living trade-offs in Ethiopian wolves
Authors: Sandoval-Serés Elisa , Foley James , Preston Elizabeth , Ebu Edris , Marino Jorgelina 
Published: November 2023

AbstractLiving in groups requires individuals to make trade-offs to maintain group cohesion and enhance individual and inclusive fitness. One way animals can do this is by adjusting the way they allocate time to different behaviours. Ethiopian wolves (Canis simensis) are highly social, cooperative breeders (only the dominant pair breeds and all members help to raise the litter), but solitary foragers. To investigate how Ethiopian wolves allocate their time to balance the costs and benefits of group living, we analysed the time budgets of 47 wolves across six behaviours. We hypothesized that group size and territory density would affect wolves’ time allocation between solitary (foraging) and communal activities (patrolling and socialising) differently in relation to their age, dominance status or sex, and whether the pack was breeding or not. Our results showed that the time spent foraging alone increased at higher territorial densities, as expected from interference competition, particularly among subadults, subordinates and individuals in breeding packs. On the other hand, as pack size increased, adult wolves spent a lower proportion of time patrolling, while the time spent socialising did not change. Living in groups reduces the costs of communal activities, but crowded territories come at the cost of interference competition for solitary foragers, particularly the animals further down the hierarchy ranks, subadults, and individuals investing in breeding. Such trade-offs could impose an upper limit to group size and population growth within a limited space. Furthermore, anthropogenic disturbance could alter the balance of social and solitary activities of Ethiopian wolves.
Comparison of endoscope-assisted and microscope-assisted type I tympanoplasty; a systematic review and meta-analysis
Authors: Elnahal Kirolos Botros , Hassan Mohamed Amir , Maarouf Ahmed Mahmoud 
Published: November 2023

Abstract
Objectives
To analyze and compare the available data about the outcomes of endoscopic and microscopic type I tympanoplasty.

Data sources
PubMed, Cochrane library Ovid, Scopus, Google scholar, and ClinicalTrials.

Methods
We conducted a meta-analysis in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. We included comparative studies describing type I tympanoplasty, and comparing surgical outcomes of the endoscope with the microscope in terms of efficacy and safety.

Results
Our systematic search yielded 22 studies meeting the inclusion criteria and eligible for analysis. The pooled graft uptake rates and audiological results of endoscopic and microscopic tympanoplasty demonstrated non-significant differences. In contrast, endoscopic type I tympanoplasty outperforms microscopic tympanoplasty regarding a highly significant decrease not only in pooled mean operative time but also in the pooled complications rate.

Conclusions
Based on our meta-analysis, the surgical outcomes of endoscope-assisted and microscope-assisted type I tympanoplasty in terms of postoperative hearing outcomes and the graft uptake rate were comparable. On the contrary, operative time and complications rate proved to be significantly reduced with endoscopy compared to microscopy. Hence, the endoscope is as efficient as the microscope in type I tympanoplasty but less invasive, fewer in complications and shorter in operative time.
Haplotype diversity patterns in Quercus suber (Fagaceae) inferred from cpDNA sequence data
Authors: Sousa Filipe , Viegas Mariana Bray , Costa Joana , Marques Isabel , Pina-Martins Francisco , Simões Fernanda , Matos José , Glushkova Maria , Miguel Célia , Veloso Maria Manuela , Oliveira M. Margarida , Ricardo Cândido Pinto , Batista Dora , Paulo Octávio S. 
Published: November 2023

AbstractChloroplast genome diversity in cork oak (Quercus suber) is characterised by the occurrence of haplotypes that are akin to those found in other Mediterranean oak species, particularly in Q. ilex and Q. rotundifolia, suggesting the possible presence of an introgressed chloroplast lineage. To further investigate this pattern, we reconstructed chloroplast haplotypes by sequencing four chloroplast markers (cpDNA), sampled across 181 individuals and 10 taxa. Our analyses resulted in the identification of two diversified chloroplast haplogroups in Q. suber, corresponding to a geographically widespread lineage and an Afro-Iberian lineage. Time-calibrated phylogenetic analyses of cpDNA point to a Miocene origin of the two haplogroups in Q. suber, suggesting that the Afro-Iberian lineage was present in cork oak before the onset of glaciation periods. The persistence of the two haplogroups in the western part of the species distribution range may be a consequence of either ancient introgression events or chloroplast lineage sorting, combined with different fixation in refugia through glaciation periods. Our results provide a comprehensive insight on the origins of chloroplast diversity in these ecologically and economically important Mediterranean oaks.
Post-radiolabeling thioether oxidation to enhance the bioorthogonal reactivity of 18F-tetrazines
Authors: Wilkovitsch Martin , Svatunek Dennis , Mikula Hannes , Denk Christoph 
Published: November 2023

AbstractRadiolabeled 1,2,4,5-tetrazines are powerful heterocyclic agents for bioorthogonal PET imaging due to their fast cycloaddition with trans-cyclooctenes. However, fluorine-18 radiolabeling of highly reactive tetrazines is often not feasible due to limited compound stability. We demonstrate that post-radiolabeling oxidation of thioether functionalities is a viable and transferable strategy to avoid these limitations, enabling the synthesis of highly reactive 18F-tetrazines.
Graphical abstract