bluuebunny/crossref_metadata_2025_split huggingface dataset:
The evolution of meandering rivers in sedimentary basins: Insights from the lower Drava (Hungary/Croatia)
Authors: Słowik Marcin , Dezső József , Salem Ali , Puhl‐Rezsek Marietta , Gałka Mariusz , Kovács János 
Published: October 2023

AbstractRivers flowing through sedimentary basins are subjected to a variety of controls. The main goal of our study was to identify the effect of external (e.g. climate changes, tectonics) and internal controls (e.g. sediment transport, deposition, vegetation cover) on the evolution of meandering rivers flowing through sedimentary basins using the example of the lower Drava River (Europe, Hungary/Croatia). Field research was conducted along a 50‐km‐long section of the valley. Sedimentary data from boreholes and corings, 35 km of ground‐penetrating radar surveys and analyses of digital maps were conducted to reconstruct channel planform changes. Traces of four meander belts were identified, and 39 AMS radiocarbon dates were used to distinguish the chronology of the fluvial events. The evolution of the lower Drava River comprised alternating periods of deposition (formation of aggrading meander belt) and avulsions. The channel belts were formed owing to upstream sediment delivery and floodplain storage. Changes in climate humidity and the occurrence of high flows influenced the planform of the meanders within particular channel belts. The oldest channel was active at least ~40 000 cal. BP before being reworked by subsequent meanders active between the Late Pleniglacial (30 000–14 700 cal. BP) and Late Glacial (~11 000 cal. BP) periods. The channel belts shifted to the south in the Holocene, between ~11 000 and 250 cal. BP due to the presence of a thrust fault situated diagonally to the Drava Valley. Results show channel width, channel belt width and the surface area of point bar deposits increased in the succeeding generations of meanders and that the style and sedimentary architecture of the channel belts were dominantly dependent on autogenic controls, that is, sediment delivery, aggradation and erosional events (e.g. formation of chute cut‐offs).
Further Limitations of Synthetic Fungicide Use and Expansion of Organic Agriculture in Europe Will Increase the Environmental and Health Risks of Chemical Crop Protection Caused by Copper-Containing Fungicides
Authors: Burandt Quentin C. , Deising Holger B. , von Tiedemann Andreas 
Published: October 2023

Abstract
Copper-containing fungicides have been used in agriculture since 1885. The divalent copper ion is a nonbiodegradable multisite inhibitor that has a strictly protective, nonsystemic effect on plants. Copper-containing plant protection products currently approved in Germany contain copper oxychloride, copper hydroxide, and tribasic copper sulfate. Copper is primarily used to control oomycete pathogens in grapevine, hop, potato, and fungal diseases in fruit production. In the environment, copper is highly persistent and toxic to nontarget organisms. The latter applies for terrestric and aquatic organisms such as earthworms, insects, birds, fish, Daphnia, and algae. Hence, copper fungicides are currently classified in the European Union as candidates for substitution. Pertinently, copper also exhibits significant mammalian toxicity (median lethal dose oral = 300–2500 mg/kg body wt in rats). To date, organic production still profoundly relies on the use of copper fungicides. Attempts to reduce doses of copper applications and the search for copper substitutes have not been successful. Copper compounds compared with modern synthetic fungicides with similar areas of use display significantly higher risks for honey bees (3- to 20-fold), beneficial insects (6- to 2000-fold), birds (2- to 13-fold), and mammals (up to 17-fold). These data contradict current views that crop protection in organic farming is associated with lower environmental or health risks. Further limitations in the range and use of modern single-site fungicides may force conventional production to fill the gaps with copper fungicides to counteract fungicide resistance. In contrast to the European Union Green Deal goals, the intended expansion of organic farming in Europe would further enhance the use of copper fungicides and hence increase the overall risks of chemical crop protection in Europe. Environ Toxicol Chem 2024;43:19–30. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Intumescent fire‐retardant performance and small‐scale reaction mechanism on banana/bio‐epoxy composites
Authors: Melati Asih , Settar Abdelhakim , Chetehouna Khaled , Foucault Léo , Sahli Mounir , Baudron Christophe 
Published: October 2023

AbstractThe present work deals with the thermal degradation, the physical characterization, and kinetic mechanisms of green epoxy resin‐based biocomposites (BC) reinforced by banana leaf fiber (BLF). The two main samples have been manufactured using the vacuum bag resin transfer molding method. The first, BLF‐based BC, is used as a control sample, while the second sample is coated with 6% wt. of intumescent fire retardant (IFR), which contains APP and boric acid. The effect of IFR coating has been investigated using thermogravimetric analysis (TGA) under inert and oxidative atmospheres and kinetic studies of model‐free and model‐based approaches have been applied for predicting the kinetic parameters of thermal degradation reactions. The TGA results show that the IFR coating delays the thermal degradation around 13–20 K of BLF‐based BC materials, which leads to an increase of 8% in the char residue under inert atmosphere. In addition, the sample has been characterized by SEM, EDS, and FTIR analysis (before and after the TGA test). The effectiveness of the IFR protective role is displayed on the SEM micrographs by showing the hole of the char enclosed and built the foam layer. The kinetic parameters from model‐free and model‐based curve fitting of BLF‐based BC and BLF‐based BC‐coated IFR are obtained, while the effect of IFR on the kinetic parameter increased the activation energy (model‐free and model‐based) under inert atmosphere. The fitting methods and optimization procedure gave excellent results for kinetic parameters, showing a particularly good correspondence with the TGA experimental data.
Neo‐Antigen‐Reactive T Cells Immunotherapy for Colorectal Cancer: A More Personalized Cancer Therapy Approach
Authors: Chen Guan‐Liang , Kong De‐Xia , Lin Yan 
Published: October 2023

AbstractColorectal cancer (CRC) is the second most common malignancy in women and the third most frequent cancer in men. Evidence has revealed that the survival of patients with metastatic CRC is very low, between one and three years. Neoantigens are known proteins encoded by mutations in tumor cells. It is theorized that recognizing neoantigens by T cells leads to T cell activation and further antitumor responses. Neoantigen‐reactive T cells (NRTs) are designed against the mentioned neoantigens expressed by tumor cells. NRTs selectively kill tumor cells without damage to non‐cancerous cells. Identifying patient‐specific and high immunogen neoantigens is important in NRT immunotherapy of patients with CRC. However, the main challenges are the side effects and preparation of NRTs, as well as the effectiveness of these cells in vivo. This review summarized the properties of neoantigens as well as the preparation and therapeutic outcomes of NRTs for the treatment of CRC.
Test–retest reliability and predictive utility of a macroscale principal functional connectivity gradient
Authors: Knodt Annchen R. , Elliott Maxwell L. , Whitman Ethan T. , Winn Alex , Addae Angela , Ireland David , Poulton Richie , Ramrakha Sandhya , Caspi Avshalom , Moffitt Terrie E. , Hariri Ahmad R. 
Published: October 2023

AbstractMapping individual differences in brain function has been hampered by poor reliability as well as limited interpretability. Leveraging patterns of brain‐wide functional connectivity (FC) offers some promise in this endeavor. In particular, a macroscale principal FC gradient that recapitulates a hierarchical organization spanning molecular, cellular, and circuit level features along a sensory‐to‐association cortical axis has emerged as both a parsimonious and interpretable measure of individual differences in behavior. However, the measurement reliabilities of this FC gradient have not been fully evaluated. Here, we assess the reliabilities of both global and regional principal FC gradient measures using test–retest data from the young adult Human Connectome Project (HCP‐YA) and the Dunedin Study. Analyses revealed that the reliabilities of principal FC gradient measures were (1) consistently higher than those for traditional edge‐wise FC measures, (2) higher for FC measures derived from general FC (GFC) in comparison with resting‐state FC, and (3) higher for longer scan lengths. We additionally examined the relative utility of these principal FC gradient measures in predicting cognition and aging in both datasets as well as the HCP‐aging dataset. These analyses revealed that regional FC gradient measures and global gradient range were significantly associated with aging in all three datasets, and moderately associated with cognition in the HCP‐YA and Dunedin Study datasets, reflecting contractions and expansions of the cortical hierarchy, respectively. Collectively, these results demonstrate that measures of the principal FC gradient, especially derived using GFC, effectively capture a reliable feature of the human brain subject to interpretable and biologically meaningful individual variation, offering some advantages over traditional edge‐wise FC measures in the search for brain–behavior associations.
Multicity comparative assessment and optimized management path of sustainability of the economy–energy–environment system: A case study of core cities in China's three major economic circles
Authors: Zhang Yujie , Ma Haichao , Wang Qingsong , Xu Yue , Tian Shu , Yuan Xueliang , Ma Qiao , Xu Yuan , Yang Shuo , Liu Chengqing 
Published: October 2023

Abstract
Coordinated and stable development of economy–energy–environment (3E) systems represents a long-term strategy for the sustainable development of humankind. Following the research idea of “indicator system construction—3E system evaluation—obstacles identification—optimization management,” this article innovatively constructs a multiangle and comparable methodology system for evaluation and optimized management of the 3E system and considers the core cities of three economic circles in China as cases for empirical research. The results show that all the coordination degree levels were of good or high quality, which was at the highest level in the country. The sustainability degree of the three cities showed an upward trend; of these, Beijing had the highest sustainability degree, followed by Guangzhou and Shanghai. Obstacle degree analysis shows that technology investment and energy factors were common factors hindering sustainable development of the 3E systems of the three cities, and each city also had its own unique factors that acted as obstacles. On this basis, this article formulates region-specific policy recommendations in order to provide a useful reference for top-level design for the government. Integr Environ Assess Manag 2024;20:875–887. © 2023 SETAC
Sclareol induces cell cycle arrest and ROS‐mediated apoptosis and ferroptosis in lung adenocarcinoma cells
Authors: Rah Bilal , Shafarin Jasmin , Hamad Mawieh , Muhammad Jibran Sualeh 
Published: October 2023

AbstractSclareol (SC) has shown significant anticancer activity against breast and colon cancers among others. However, its ability to precipitate similar anticancer effects in lung cancer has yet to be investigated. To address this issue, SC‐treated lung adenocarcinoma cells (A549) were assessed for viability and functional competence as well as the expression of genes related to apoptosis and cell cycling. Our results demonstrated that SC treatment inhibited A549 cell clonogenic features and reduced their migration and invasion potential in a dose‐dependent manner. Mechanistically, SC treatment downregulated the expression of cyclin D1 and survivin and upregulated that of p21 and p16, which was associated with a significant increase in the percentage of SubG0 cells. SC treatment is also associated with the induction of both the extrinsic and intrinsic apoptotic pathways, as evidenced by the increased expression and splitting of PARP1 and procaspases 3 and 9 and the reduced expression of antiapoptotic proteins Bcl‐2 and Bcl‐xL. Increased cell death in SC‐treated cells is likely to have resulted from the induction of ferroptosis as suggested by the reduced expression of FPN and the inhibition of the anti‐ferroptosis regulator GPX4. In conclusion, the data presented here suggest that SC can reduce lung carcinoma cell growth and metastasis and promote cell death.
Associations between therapeutic working alliance, meaningful experiences, and affective distress
Authors: Cook Ryan M. , Sackett Corrine R. , Fye Heather J. 
Published: October 2023

AbstractWe used structural equation modeling to test client meaningful experiences in counseling and the therapeutic working alliance (TWA) as predictors of affective distress, controlling for clients’ age, gender, race and ethnicity, and length of counseling relationship. In a sample of 306 adult clients engaging in counseling, we a found statistically significant relationship between client meaningful experiences in counseling and the TWA (r = 0.80, p < 0.001). There was a significant and negative direct effect from client meaningful experiences in counseling to affective distress (β = −0.45, p < 0.001). However, we did not find a significant direct effect of the TWA on affective distress (β = −0.00, p = 0.97). Implications for counselors, supervisors, and counselor educators are discussed.
Association between body roundness index and non‐alcoholic fatty liver disease detected by Fibroscan in America
Authors: Jiang Ningning , Zhang Shengguo , Chu Jinguo , Yang Naibin , Lu Mingqin 
Published: October 2023

AbstractBackgroundThe prevalence of non‐alcoholic fatty liver disease (NAFLD) and obesity is worldwide on the rise. Body roundness index (BRI), as a newly developed anthropometric indicator, has been recently reported to identify obesity. However, it is still unclear whether BRI is associated with the prevalence of NAFLD.MethodsData were from the National Health and Nutrition Examination Survey (NHANES) 2017–2018. NAFLD was diagnosed based on hepatic steatosis defined by CAP values ≥274 dB/m. Multivariable logistic regression analysis was performed to detect the association between BRI and the odds of NAFLD. Subgroup analysis stratified by age, gender, BMI, and race was further conducted. To explore the potential ability of BRI in predicting NAFLD, the area under the curve (AUC) of BRI was calculated by receiver operating characteristic (ROC) analysis.ResultsAmong the 4467 study participants, 1718 (38.5%) were diagnosed with NAFLD. Compared to the non‐NAFLD group, participants with NAFLD had a higher level of BRI. The positive associations between BRI and NAFLD were detected in all three models (mode 1: OR = 1.71, 95% CI: 1.65–1.78, p < 0.0001; mode 2: OR = 1.78, 95% CI: 1.71–1.86, p < 0.0001; mode3: OR = 1.23, 95% CI: 1.11–1.35, p < 0.0001). The positive association steadily existed in different subgroups after stratified by age, gender, and BMI. Moreover, the non‐linear association between BRI and NAFLD was detected, presenting inverted U‐shaped curves. Furthermore, BRI had a high predictive value (AUC = 0.807) in identifying NAFLD.ConclusionsBRI was positively associated with the prevalence of NAFLD among individuals in America, regardless of age, gender, and BMI. Besides, BRI presented a high ability for identifying NAFLD.
Complex genotypes in family with metachromatic leukodystrophy: Effect of trans and cis mutations distribution on the phenotype variability
Authors: Ben Issa Abir , Kamoun Fatma , Bouchaala Wafa , Charfi Triki Chahnez , Fakhfakh Faiza 
Published: October 2023

AbstractMetachromatic leukodystrophy (MLD) is a severe metabolic disorder caused by the deficient activity of arylsulfatase A due to ARSA gene mutations. According to the age of onset, MLD is classified into three forms: infantile, juvenile, and adult. In our study, we aimed to perform a genetic analysis for two siblings with juvenile MLD for a better characterization of the molecular mechanisms behind the disease. A consanguineous family including two MLD patients (PII.1 and PII.2) was enrolled in our study. The diagnosis was made based on the clinical and neuroimaging investigations. The sequencing of ARSA gene was performed followed by in silico analysis. Besides, the cis/trans distribution of the variants was verified through a PCR‐RFLP. The ARSA gene sequencing revealed three known variants, two exonic c.1055A > G and c.1178C > G and an intronic one (c.1524 + 95A > G) in the 3′UTR region. All variants were present at heterozygous state in the two siblings and their mother. The assessment of the cis/trans distribution showed the presence of these variants in cis within the mother, while PII.2 and PII.2 present the c.1055A > G/c.1524 + 95A > G and the c.1178C > G in trans. Additionally, PII.1 harbored a de novo novel missense variant c.1119G > T, whose pathogenicity was supported by our predictive results. Our genetic findings, supported by a clinical examination, confirmed the affection of the mother by the adult MLD. Our results proved the implication of the variable distribution of the found variants in the age of MLD onset. Besides, we described a variable severity between the two siblings due to the de novo pathogenic variant. In conclusion, we identified a complex genotype of ARSA variants within two MLD siblings with a variable severity due to a de novo variant present in one of them. Our results allowed the establishment of an adult MLD diagnosis and highlighted the importance of an assessment of the trans/cis distribution in the cases of complex genotypes.
A team‐based approach to cardiogenomic education
Authors: Edelman Emily A. , Logan Kalisi , Ingram Nissen Therese , Reed E. Kate , Wicklund Catherine , Duquette Debra , Dellefave‐Castillo Lisa 
Published: October 2023

AbstractWhile many genetic professionals are involved in the education of lay and professional audiences, most do not have formal training in education theory and program design. Partnerships with adult education experts can provide additional resources and improve the level of instruction, thereby increasing the impact of an educational intervention. This report discusses the experience of a multidisciplinary team of educators, clinicians, and researchers partnering to develop evidence‐based education for cardiology practitioners. It includes practical advice for how clinicians and educators can develop more effective education through collaboration, needs assessment, instructional design, and iterative content development.
Identification of SGMS2 as a molecule involved in natural killer cell recruitment and its in‐deep analysis in the liver cancer microenvironment: Evidence from large populations cohort
Authors: Tang Wenqing , Meng Fansheng , Li Miao , Li Yuqin , Xiong Wujun , Zhang Danying , Yang Biwei 
Published: October 2023

AbstractBackgroundLiver cancer, a common malignancy within the digestive system, presents with a particularly grim prognosis. Within the immune microenvironment, the role of natural killer (NK) cells in liver cancer remains unclear.MethodsWe sourced data on clinical parameters and gene expressions for liver cancer patients from The Cancer Genome Atlas Program database and carried out all analyses using R software and its relevant codes.ResultsIn our research, we delved into the genes intertwined with NK cells in hepatocellular carcinoma (HCC). Leveraging the QUANTISEQ and MCPCOUNTER algorithms to quantify NK cells, we spotlighted genes vital to the recruitment of NK cells. Among these genes, GDE1, WDFY3, DNAJB14, PKD2, DGAT2, SGMS2 and MKNK2 showed a strong correlation with patient outcomes. We also mapped out the single‐cell expression trajectories of these genes within the HCC milieu. From our findings, SGMS2 emerged as a key gene warranting further scrutiny. Our in‐depth analysis of SGMS2 shed light on its influence over specific biological pathways, its contribution to the immune landscape and its role in genomic instability within HCC. Drawing from this, we formulated a predictive model rooted in SGMS2‐associated genes. This model showcased remarkable precision across both training and validation cohorts.ConclusionsOverall, our investigation underscored the profound implications of SGMS2, a gene pivotal to NK cell infiltration, in the landscape of HCC, thereby positioning it as a potential linchpin in oncological strategies.
Findings from the Tushirikiane mobile health (mHealth) HIV self‐testing pragmatic trial with refugee adolescents and youth living in informal settlements in Kampala, Uganda
Authors: Logie Carmen H. , Okumu Moses , Berry Isha , Hakiza Robert , Baral Stefan D. , Musoke Daniel Kibuuka , Nakitende Aidah , Mwima Simon , Kyambadde Peter , Loutet Miranda , Batte Shamilah , Lester Richard , Neema Stella , Newby Katie , Mbuagbaw Lawrence 
Published: October 2023

AbstractIntroductionUrban refugee youth remain underserved by current HIV prevention strategies, including HIV self‐testing (HIVST). Examining HIVST feasibility with refugees can inform tailored HIV testing strategies. We examined if HIVST and mobile health (mHealth) delivery approaches could increase HIV testing uptake and HIV status knowledge among refugee youth in Kampala, Uganda.MethodsWe conducted a three‐arm pragmatic controlled trial across five informal settlements grouped into three sites in Kampala from 2020 to 2021 with peer‐recruited refugee youth aged 16–24 years. The intervention was HIVST and HIVST + mHealth (HIVST with bidirectional SMS), compared with standard of care (SOC). Primary outcomes were self‐reported HIV testing uptake and correct status knowledge verified by point‐of‐care testing. Some secondary outcomes included: depression, HIV‐related stigma, and adolescent sexual and reproductive health (SRH) stigma at three time points (baseline [T0], 8 months [T1] and 12 months [T2]). We used generalized estimating equation regression models to estimate crude and adjusted odds ratios comparing arms over time, adjusting for age, gender and baseline imbalances. We assessed study pragmatism across PRECIS‐2 dimensions.ResultsWe enrolled 450 participants (50.7% cisgender men, 48.7% cisgender women, 0.7% transgender women; mean age: 20.0, standard deviation: 2.4) across three sites. Self‐reported HIV testing uptake increased significantly from T0 to T1 in intervention arms: HIVST arm: (27.6% [n= 43] at T0 vs. 91.2% [n= 135] at T1; HIVST + mHealth: 30.9% [n= 47] at T0 vs. 94.2% [n= 113] at T1]) compared with SOC (35.5% [n= 50] at T0 vs. 24.8% [ = 27] at T1) and remained significantly higher than SOC at T2 (p<0.001). HIV status knowledge in intervention arms (HIVST arm: 100% [n= 121], HIVST + mHealth arm: 97.9% [n= 95]) was significantly higher than SOC (61.5% [n= 59]) at T2. There were modest changes in secondary outcomes in intervention arms, including decreased depression alongside increased HIV‐related stigma and adolescent SRH stigma. The trial employed both pragmatic (eligibility criteria, setting, organization, outcome, analysis) and explanatory approaches (recruitment path, flexibility of delivery flexibility, adherence flexibility, follow‐up).ConclusionsOffering HIVST is a promising approach to increase HIV testing uptake among urban refugee youth in Kampala. We share lessons learned to inform future youth‐focused HIVST trials in urban humanitarian settings.