bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial heterogeneity facilitates coexistence between striped hyaenas and sympatric carnivores
Authors: Ashish K. , Ramesh T. , Kalle Riddhika , Giordano Anthony J. 
Published: October 2023

AbstractAimInterspecific competition plays a key role in shaping carnivore communities. Top‐down effects can impact the coexistence of superordinate and subordinate competitors and prey across a shared landscape. Limited resources, their abundance and diminishing habitats can all exacerbate interspecific competition. Sometimes, behavioural mechanisms or adaptations are critical for subordinate carnivores to coexist with larger species. Here, we investigated the distribution pattern of the striped hyaena (Hyaena hyaena) population with respect to habitat features, sympatric carnivores and their prey species across a large landscape.LocationWestern and Eastern Ghats, India.TaxonStriped hyaena.MethodsWe collected presence–absence data for hyaenas, tigers, leopards, dholes and their prey using camera‐trapping and indirect sign surveys. Using a random forest algorithm, we modelled the local distribution of hyaenas as a function of important ecological variables, including climate, domestic prey abundance and the predicted probabilities of occurrence of other large carnivores and their prey.ResultsWe found that striped hyaenas were confined to relatively small, discrete areas compared to the three other predators, likely a mechanism for achieving coexistence in the landscape. Our empirical results suggested that hyaenas tend to avoid high occurrence probability areas of their co‐predators at a landscape level but foothold opportunities such as, arid climatic conditions, and prey abundance primarily determined spatial incumbency. Coexistence in an area with low to medium relative abundance of co‐predators offsets the interplay between carcass acquisition and risk associated. The coexistence of species was favoured by altitudinal heterogeneity probably providing denning refugia and also by their preference towards suboptimal thorn forests that sustain a low density of co‐predators.Main ConclusionsOur study showed a desisting impact of large predators on the distribution of the striped hyaena population. Furthermore, we suggest preventing habitat alteration in suboptimal habitats of large carnivores to facilitate coexistence in a shared landscape.
Isolating the drivers of the species‐area relationship in experimental habitat islands
Authors: Hanks Granger W. , Clay Natalie A. , Herrmann Maggie , Nunnally Clifton , Bryant S. River D. , McClain Craig R. 
Published: October 2023

AbstractAimConsidered one of ecology's laws, the species‐area relationship (SAR) is among the most intensively studied ecological phenomena, yet the mechanisms through which the SAR arises and under what circumstances remain poorly understood. Here, we experimentally test the effects of area alone in the absence of commonly invoked mechanistic drivers of the SAR in both sub‐tidal bay and inter‐tidal marsh habitats.LocationLouisiana, U.S.A.TaxonInvertebrate macrofauna.MethodsTo determine if surface area alone may generate the SAR, we conducted a small‐scale, ephemeral habitat island experiment in a subtidal soft‐bottom bay and in an intertidal soft‐bottom coastal salt marsh. Cellulose sponge habitat islands of varying surface areas, but with constant energy content and habitat diversity, were deployed in each location to isolate the effects of surface area on the SAR in the absence of species‐energy relationships and habitat diversity.ResultsArea alone, likely through passive sampling, generated significant SARs in both habitats, with the salt marsh producing the strongest relationship, likely due to differential physical structuring between habitats. Communities of low surface area sponges were generally nested within those of high surface area sponges, and dispersal limitation may have increased the importance of nestedness relative to species replacements in structuring communities between habitats.Main ConclusionsThat area alone, in the absence of covariates such as energy availability and habitat diversity may, significantly impact the species richness of experimental and ephemeral marine benthic habitat island communities. Our methodology may provide a new avenue for SAR research, allowing for the isolation and simultaneous testing of potential drivers through the physical manipulation of ephemeral habitats.
Parental practices and children's lifestyle correlates of childhood overweight/obesity in Europe: The Feel4Diabetes study
Authors: Kontochristopoulou Aikaterini M. , Karatzi Kalliopi , Karaglani Eva , Cardon Greet , Kivelä Jemina , Iotova Violeta , Tankova Tsvetalina , Rurik Imre , Radone Anett S. , Liatis Stavros , Makrilakis Konstantinos , Moreno Luis A. , Manios Yannis 
Published: October 2023

AbstractBackgroundParental influences on children's eating and physical activity (PA) and consequently on their weight are fundamental. The present study aimed to identify the predominant correlates of childhood overweight/obesity among a variety of parental practices and children's lifestyle indices in a large sample of children in Europe.MethodsFamilies from low socio‐economic status regions were recruited through schools, located in six European countries (Belgium, Finland, Greece, Spain, Bulgaria and Hungary). Seven thousand three hundred ninety‐seven children 4–12 years old and their parents were selected using the FINDRISC‐questionnaire. Parental practices assessed included parental role modelling, permissiveness and reward. Children's dietary intake and lifestyle behaviours were assessed through parent‐reported questionnaires.ResultsRegarding parental practices, it was revealed that being sometimes (odds ratio [OR] = 1.26; 95% confidence interval [CI] = 1.10–1.43) or rarely (OR = 1.43; 95% CI = 1.21–1.69) physically active with the child was associated with greater overweight/obesity risk, whereas rare permission of computer/mobile/tablet (OR = 0.81; 95% CI = 0.67–0.98) and sometimes (OR = 0.77; 95% CI = 0.68–0.88) or rare (OR = 0.77; 95% CI = 0.66–0.91) reward with PA were associated with lower risk. Regarding children's lifestyle factors, consuming > 3 cups/week fresh fruit juices (OR = 1.28; 95% CI = 1.13–1.45), skipping breakfast (OR = 1.37; 95% CI = 1.17–1.61), absence of 1 h of daily PA (OR = 1.40; 95% CI = 1.24–1.58) and increased daily screen time (ST) (OR = 1.23; 95% CI = 1.09–1.39) were associated with greater overweight/obesity risk. All the variables were adjusted for maternal education, child's sex and age.ConclusionsThese findings emphasize the necessity of family‐centered approaches in health promotion and obesity prevention programs for children. Such programs should focus on parents as the primary role models in exerting positive influence and encouraging healthy eating habits, PA, and ST behaviors in their children, which in turn, may have a substantial impact on children's overall weight status.
Associations between food insecurity and child and parental physical, nutritional, psychosocial and economic well‐being globally during the first 1000 days: A scoping review
Authors: Rosen Francesca , Settel Lily , Irvine Faith , Koselka Elizabeth P. D. , Miller Joshua D. , Young Sera L. 
Published: October 2023

AbstractFood insecurity affects billions of individuals annually and contributes to myriad poor health outcomes. Experiences of food insecurity may be particularly harmful during the first 1000 days, but literature on the topic has not been synthesized. We therefore aimed to characterize all available studies examining associations between food insecurity and nutritional, psychosocial, physical and economic well‐being among parents and children during this period. We implemented a standardized search strategy across 11 databases. Four researchers screened 10,257 articles, 120 of which met the inclusion criteria. Most studies were conducted in Sub‐Saharan Africa (43.3%), followed by North America (20.8%). Studies were primarily quantitative (95.8%), cross‐sectional (70.0%) and focused on women (pregnant or post‐partum, 48.3%) or women and children (15.8%). Physical health outcomes were the most investigated (n = 87 studies), followed by nutritional (n = 69), psychosocial (n = 35) and economic well‐being (n = 2). The most studied associations were between food insecurity and stunting (n = 15), maternal depression (n = 12), child dietary diversity (n = 7) and maternal body mass index (n = 6). The strength of evidence for the observed associations varied across populations as well as within and between examined outcomes. We recommend that future studies recruit more diverse study populations, consider temporality of relationships, use instruments that facilitate cross‐site comparisons, measure individual‐level food insecurity and outcomes most likely to be impacted by food insecurity, evaluate contextual factors that may modify the effects of food insecurity and employ analytic techniques that permit assessment of causal pathways.
Feasibility Study on the Application of Boron Carbide for Long Term Reactivity Control in the LOTUS Small Fast Reactor
Authors: Vu Thanh Mai , Bui Thi Hong , Tran Le Quang Linh 
Published: October 2023

Abstract
200 MWth lead-cooled fast reactor (LOTUS) reactor core is a small modular lead-cooled fast reactor with designed power of 200 MWth under development at Vietnam National University (VNU) University of Science, Hanoi for a floating nuclear power plant application. For that purpose, advanced passive safety features and no refueling requirement are the priorities in the core design process. To endure the continuous operation over a long lifetime, the startup core exhibits excess reactivity to cover the reactivity loss due to burnup. The reactivity control system includes burnable poison and absorber rods and layers made of B4C which are employed in the reactor to minimize the excess reactivity of the core to about 1 $ to enhance the safety features of the core. The burnable poison is fixed inside the reactor while absorber rods/absorber layers are withdrawn or inserted in sequence to achieve the required excess reactivity of about 700 pcm. The reactivity control was arranged into ten steps to achieve the operating time of 15 effective full-power years without refueling. Good neutronic behavior of the core was observed with negative fuel temperature coefficient and coolant void reactivity and maximum radial power peaking factor of 1.32. However, a quite large residual absorption caused by fixed burnable poison inside fuel assemblies was revealed. In further study, to increase the neutron absorption efficiency of burnable poison in the fast spectrum as well as the reactor lifetime, a neutron moderator will be considered to add to the burnable poison rods.
Whole-genome sequences of two
Drosophila melanogaster
microbiome symbionts
Authors: Barron Alexander J. , Broderick Nichole A. 
Published: October 2023

ABSTRACT

Lactiplantibacillus plantarum
and
Acetobacter tropicalis
are bacterial symbionts commonly isolated from decaying fruits and from the microbiome of
Drosophila melanogaster
. Studies have shown that these organisms interact synergistically, imparting beneficial effects on the host. Here, we report whole-genome sequences of these microbes obtained from long and short reads.
Draft genome sequence of the BAL58 Betaproteobacteria representative strain LSUCC0117
Authors: Stapelfeldt Holly R. D. , Lanclos V. Celeste , Henson Michael W. , Thrash J. Cameron 
Published: October 2023

ABSTRACT
Here, we present the draft genome sequence of strain LSUCC0117, a representative of the abundant aquatic BAL58 Betaproteobacteria group which we isolated from a coastal site in the northern Gulf of Mexico. The genome is estimated at over 99% complete, with a genome size of 2,687,225 bp.
Tegument Ultrastructure and Morphology of the Capsule Surrounding the Tetrathyridia of the Genus Mesocestoides Vaillant, 1863 in the Liver of the Root Vole
Authors: Pospekhova N. A. , Kusenko K. V. 
Published: October 2023

Abstract
The ultrastructure of the tegument of encapsulated tetrathyridia of the genus Mesocestoides Vaillant, 1863 (Cestoda, Cyclophyllidea, Mesocestoididae) from the liver of root voles Microtus oeconomus (Pallas, 1776) and the structure of the three-layered capsule surrounding them were studied for the first time. Several types of extracellular structures were noted on the surface of the tetrathyridia tegument: vesicles, fine granular material, and vacuoles. In addition, the phenomenon of shedding microtriches, which have expanded parts, was found. Host cells in contact with extracellular material show signs of destruction. A characteristic feature of the capsules surrounding the tetrathyridia is the reticular structure of the fibrous layer containing both native and degenerating inflammatory cells.
Approbation of a New Model of Secondary Damage after Traumatic Brain Injury Based on Reprogrammed Rat Embryo Fibroblasts
Authors: Rykunova E. B. , Mikeladze M. A. , Utepova I. A. , Chupakhin O. N. , Guzhova I. V. , Lazarev V. F. 
Published: October 2023

Abstract
The paper presents a new model of secondary injuries after traumatic brain injury. The model is based on the cultivation of rat embryonic fibroblasts reprogrammed to a neuronal phenotype in the presence of cerebrospinal fluid from injured rats. The presented model was used to test the therapeutic effect of inducers of the synthesis of chaperones from the classes of pyrrolylazines and indolylazines, which have neuroprotective properties.
Helicity amplitudes without gauge cancellation for electroweak processes
Authors: Chen Junmou , Hagiwara Kaoru , Kanzaki Junichi , Mawatari Kentarou 
Published: October 2023

AbstractIn the 5-component representation of weak bosons, the first four components make a Lorentz four vector, representing the transverse and longitudinal polarizations excluding the scalar component of the weak bosons, whereas its fifth component corresponds to the Goldstone boson. We obtain the $$5\times 5$$

5
×
5

component propagators of off-shell weak bosons, proposed previously and named after the Goldstone boson equivalence theorem, by starting from the unitary-gauge representation of the tree-level scattering amplitudes, and by applying the BRST (Becchi–Rouet–Stora–Tyutin) identities to the two sub-amplitudes connected by each off-shell weak-boson line. By replacing all weak boson vertices with those among the off-shell 5-component wavefunctions, we arrive at the expression of the electroweak scattering amplitudes, where the magnitude of each Feynman amplitude has the correct on-shell limits for all internal propagators, and hence with no artificial gauge cancellation among diagrams. Although our derivation is limited to the tree-level only, it allows us to study the properties of each Feynman amplitude separately, and then learn how they interfere in the full amplitudes. We implement the 5-component weak boson propagators and their vertices in the numerical helicity amplitude calculation code (Helicity Amplitude Subroutines), so that an automatic amplitude generation program such as can generate the scattering amplitudes without gauge cancellation. We present results for several high-energy scattering processes where subtle gauge-theory cancellation among diagrams takes place in all the other known approaches.
A Survey of Deep Learning for Low-shot Object Detection
Authors: Huang Qihan , Zhang Haofei , Xue Mengqi , Song Jie , Song Mingli 
Published: October 2023

Object detection has achieved a huge breakthrough with deep neural networks and massive annotated data. However, current detection methods cannot be directly transferred to the scenario where the annotated data is scarce due to the severe overfitting problem. Although few-shot learning and zero-shot learning have been extensively explored in the field of image classification, it is indispensable to design new methods for object detection in the data-scarce scenario, since object detection has an additional challenging localization task. Low-Shot Object Detection (LSOD) is an emerging research topic of detecting objects from a few or even no annotated samples, consisting of One-Shot Object Localization (OSOL), Few-Shot Object Detection (FSOD), and Zero-Shot Object Detection (ZSOD). This survey provides a comprehensive review of LSOD methods. First, we propose a thorough taxonomy of LSOD methods and analyze them systematically, comprising some extensional topics of LSOD (semi-supervised LSOD, weakly supervised LSOD, and incremental LSOD). Then, we indicate the pros and cons of current LSOD methods with a comparison of their performance.Finally, we discuss the challenges and promising directions of LSOD to provide guidance for future works.
Abstract P2007: Novel Regulatory Axis In Heart-adrenal Cross Talk Mediated By Mtor-eprs
Authors: Liu Tian , Zhang Ye , Yu Junyi , Ren Shuxun , Zhu Cansheng , Rau Christoph D , Wang Yibin , Gao Chen 
Published: October 2023

Adrenal catecholamines, including norepinephrine and epinephrine play an important role in cardiovascular physiology. Serum catecholamine level is tightly regulated in response to metabolic and cardiac stress. The biosynthesis of catecholamine is driven by a series of enzymatic reaction carried out by the key rate-limiting enzymes, including tyrosine hydroxylase (TH), DOPA decarboxylase (DDC) and phenylethanolamine-N-methyltransferase (PNMT). Although the transcription regulation of these enzymes has been described before, the regulatory mechanism at post-transcription or protein translation level has never been explored.
Using a system genetics approach, we explored genes associated with the adrenal gland traits in HMDP at basal and post isoproterenol treatment. We have identified EPRS (Glutamyl-Prolyl-tRNA synthetase) to be significantly associated with adrenal gland mass post ISO treatment. EPRS is a tRNA synthetase responsible for charging tRNA during protein translation, EPRS also regulates mRNA translation as an RNA binding protein. To explore the functional impact of EPRS in adrenal gland physiology, we generated an EPRS adrenal gland specific knockout mouse model (EPRS-aKO). Inactivation of EPRS in adrenal gland led to a decrease of adrenal gland mass associated with reduced systemic catecholamine level. Interestingly, we further observed a moderate yet significantly decreased heart rate and ejection fraction in EPRS-aKO mice. Through both in vivo and in vitro analysis, we confirmed inactivation of EPRS led to a reduced protein, but not mRNA expression for both TH and DDC. In adipose tissue, activation of EPRS through mTOR signaling pathway is critical for its function as RNA binding protein. To further explore the mechanism through EPRS mediated catecholamine synthesis, we treated PC12 cells with rapamycin to inhibit mTOR activity. Treatment with Rapamycin diminished EPRS activity and expression, reduced TH protein expression along with a decrease of catecholamine level.
Our results identified a novel regulatory scheme of catecholamine synthesis through mTOR-EPRS axis, adding a novel layer of gene regulation in catecholamine production and providing new mechanistic insights for heart-adrenal cross talk.
Abstract P3002: Role Of Vascular Fibroblast Growth Factor Signaling In Group 3 Hypoxia-induced Pulmonary Hypertension
Authors: Woo Kel Vin , Weinheimer Carla , Nigro Jess , Kovacs Attila , Ornitz David 
Published: October 2023

Pulmonary hypertension (PH) is a debilitating disease characterized by pathologic changes in the pulmonary vasculature that increases pulmonary vascular resistance and leads to right heart failure. Group 3 PH is caused by lung disease and/or hypoxia, including bronchopulmonary dysplasia (BPD) and chronic obstructive lung disease (COPD), and is the second largest cause of mortality in PH patients. PH further increases the morbidity and mortality in both COPD and BPD patients.
Fibroblast Growth Factor 2 (FGF2) and FGF Receptor 1 (FGFR1) expression is elevated in lung tissue of PH patients. To study the role of FGF signaling in the pathogenesis of group 3 PH, we developed a physiologically relevant mouse model in which mice are challenged with 10% hypoxia for 2 weeks. Following hypoxia exposure, cardiac catheterization is used to measure right ventricular (RV) pressure that mimics Group 3 PH. Lung tissue is then analyzed for histological and molecular changes.

Our initial work showed that inactivation of FGFRs in endothelial cells (EC) worsened hypoxia-induced PH while expression of a constitutively active FGFR1 (caFGFR1) in ECs protected against hypoxia-induced PH. Here we hypothesize that FGF signaling may also affect the pathogenic response to hypoxia of vascular smooth muscle (VSMC), potentially by limiting vascular muscularization in response to hypoxia. We show that
in vitro
inhibition of FGF2 signaling in human pulmonary artery VSMCs reduced hypoxia-induced proliferation while promoting a contractile phenotype, and
in vivo
inactivation of FGFRs 1 and 2 worsened hypoxia-induced vascular remodeling. These data suggest the potential for a dual beneficial effect of activation of FGF signaling, both in regulating EC-VSMC interactions and directly preventing VSMC remodeling.
Abstract P3162: Evaluating Cargo And Cardioprotective Effects Of Extracellular Vesicles From Human Right Atrial Appendage Mesenchymal Cells
Authors: Czosseck Andreas , Chen Max M , Hsu Chuan-Chih , Meeson Annette , Oldershaw Rachel , Lundy David 
Published: October 2023

Our team has previously shown the isolation and culture of a mesenchymal cell population from the right atrial appendage (RAA) of living human donor tissues. The cells are CD44/105/166
+
, CD45/19/11b
-
and secrete growth factors (including VEGF and IGF-1) comparable to BM-MSCs. Their secretome preserved EF% and dP/dTmax in a mouse MI model and stimulated angiogenesis in a mouse limb ischemia model.

In the current study, we specifically investigated using these cell-derived extracellular vesicles (EV) as a therapeutic. First, we validated our isolation technique and profiled EV miRNA cargo using PCR arrays. We then assessed the ability of EVs to protect human cardiomyocytes in a hypoxic (1% O2) hiPSC-CM model. Lastly, we used RNA-seq to compare the transcriptome of EV-treated hiPSC-CMs against hypoxic and normoxic controls.

Results confirmed isolation of high purity EVs, showing (by cryoEM) ~120 nm bilayer membrane-bound vesicles, positive for CD81, CD63, ALIX, TSG101 and other EV markers (by antibody array). In the EV cargo we identified 363 known miRNAs present in 3 donor cell isolates, including hsa-miR-21-5p, -202-5p, -1260a and -125b. Target prediction of the most abundant miRNAs ranked GO:0010667 “negative regulation of cardiac muscle cell apoptotic process” as highly significant (26.3%, P = 1.6 x10
-8
), as well as other predicted pathways related to angiogenesis, cardiac development and regulation of inflammation.

A single, transient, treatment of donor EVs (66 ng EV protein/μl) reduced LDH leakage from hypoxic hiPSC-CMs, preserved morphology, and increased viability at 48 hrs and 7 days after injury. RNA-seq of the EV-treated hiPSC-CMs revealed strong upregulation of pathways related to PI3k-akt, calcium signaling and ECM binding compared to hypoxic hiPSC-CMs with vehicle control. We are currently validating key RNA-seq results.
In conclusion, we analyzed and confirmed cardioprotective properties of EVs derived from human RAA samples using a human model system. Combined with our published animal studies, these data indicate that this cell population is a clinically-relevant source of donor cells for cardiac cell therapy. The EVs are also an attractive cell-free potential therapeutic option.