bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rate of Decrease in Serum Autotaxin Can Predict Relapse of Type 1 Autoimmune Pancreatitis After Initial Steroid Therapy
Authors: Horiuchi Ichitaro , Nakamura Akira , Kuraishi Yasuhiro , Sasaki Nobukazu , Minamisawa Masafumi , Kamakura Masato , Kondo Shohei , Yanagisawa Takumi , Oguchi Takaya , Nagaya Tadanobu , Shimamoto Satoshi , Igarashi Koji , Umemura Takeji 
Published: February 2024

Abstract
Objectives: IgG4 has been reported as a predictive marker of relapse in autoimmune pancreatitis (AIP). Serum autotaxin (ATX) was also recently found to associate with AIP relapse. This study examined the changes in serum IgG4 and ATX levels for predicting relapse in AIP patients after initial steroid therapy.
Methods: Patients with AIP who had received initial steroid therapy were retrospectively enrolled. Serum IgG4 and ATX levels were measured before and after treatment. The rates of decrease (Δ) in serum marker levels were calculated by dividing the difference between before and after steroid therapy by the number of days between them.
Results: The 37 AIP patients analyzed included 26 non-relapse and 11 relapse patients. The median Δ of serum IgG4 was 5.19 (interquartile range [IQR]: 2.65-14.49) in the non-relapse group and statistically comparable to the 3.07 (IQR: 1.79-4.43) in the relapse group (P=0.12). The median Δ of serum ATX was 1.32×10-3 (IQR: 0.23×10-3-2.45×10-3) in the non-relapse group and 0.58×10-3 (IQR: -1.34×10-3-0.91×10-3) in the relapse group, which was a significant difference (P<0.01). Positive predictive values (PPVs) for relapse were 70.1% for the Δ of IgG4 and 67.6% for the Δ of ATX. The PPV at either the Δ of IgG4 or the Δ ATX cut-off was 86.5%. In the 10 cases of IgG4 <270 mg/dL, the PPV for the Δ of IgG4 was 20%, while that for the Δ of ATX was 70%.
Conclusions: The combination of IgG4 and ATX decrease rates may help predict relapse in AIP patients after steroid therapy.
High Prevalence of Uncontrolled Asthma and Its Association with Obesity and GERD-related symptoms in Syria: A Multicenter Cross-Sectional Study
Authors: Bakdounes Duaa , Dughly Ruba , Almasri Imad-Addin , Martini Nafiza , Hanna Majd , Albelal Douaa , bardan Hussam Al 
Published: February 2024

Abstract
Introduction
Asthma control remains suboptimal globally, with high rates of partly and uncontrolled disease. Poor asthma control can be attributed to the severity of the disease, ineffective treatment, and the presence of comorbid conditions such as obesity and gastroesophageal reflux disease (GERD).
Objective
This cross-sectional study represents the first investigation in Syria to explore the relationship between asthma control status and common asthma comorbidities, namely obesity and gastroesophageal reflux disease (GERD).
Methods
This multicenter cross-sectional study interviewed 275 asthma patients from respiratory clinics at four hospitals using questionnaires on sociodemographic, asthma control per Global Initiative for Asthma (GINA) guidelines, symptoms, and management. Body Mass Index (BMI), waist-to-hip (WHR) and waist-to-height ratios (WHtR) were calculated. Associations were assessed between asthma control, obesity markers, GERD symptoms, and other variables.
Results
Most patients were women (72%) with a mean age of 41 years. Based on GINA criteria, 60% had uncontrolled, 28.7% partly controlled, and only 11.3% well controlled asthma. Higher BMI associated significantly with worse control (p = 0.006). WHtR also correlated with poorer asthma control (p < 0.001). While GERD diagnosis did not relate significantly to asthma control, symptoms like heartburn, chest pain, and chronic cough did (p < 0.05), as did the lack of GERD treatment (p = 0.002). Additionally, the lack of reflux treatment correlated with poorer asthma control (p = 0.002).
Conclusion
There is a marked prevalence of inadequately controlled asthma in Syria. Both obesity and GERD-related symptoms correlate with poorer asthma control, emphasizing the need for a comprehensive management strategy.
SHCBP1 Promotes Cisplatin Resistance of Ovarian Cancer Through AKT/mTOR/Autophagy Pathway
Authors: Qi Gonghua , Ma Hanlin , Teng Kai , Gong Yanmin , Chen Jingying , Luo Xia , Kong Beihua , Gai Panpan 
Published: February 2024

Abstract

Ovarian cancercaused the highest cancer-related mortality among female reproductive system malignancies. Platinum-based chemotherapy is still the footstone of the chemotherapy for ovarian cancer. However, the molecular mechanisms underlying cisplatin insensitivity and resistance remain unclear. SHC SH2 domain-binding protein 1 (SHCBP1) plays critical roles in the progression and drug resistance of different types of cancer. However, the biological function of SHCBP1 in ovarian cancer progression and cisplatin resistance remains obscure. In this study, we found that SHCBP1 was up-regulated in ovarian cancer and the up-regulated SHCBP1 has growth-promoting effect on ovarian cancer cells. Furthermore, SHCBP1silencing sensitize ovarian cancer cells to CDDP. Mechanism analysis revealed that SHCBP1 activated the Akt/mTOR pathway and further inhibited autophagy in ovarian cancer cells. Meanwhile, Autophagy inhibitors combined with SHCBP1 knockdown enhances CDDP sensitivity. In addition, SHCBP1 inhibition restrained the proliferation of tumorsand increased the cisplatin sensitivity in vivo. These findings suggested that up-regulated SHCBP1 promoted the proliferation and CDDP resistance of ovarian cancer.The combination of SHCBP1 inhibition and cisplatin treatment might lead to substantial progress in ovarian cancer targeted therapy.
Apatinib combined with an anti-PD-1 agent promotes antitumour immunity in mice with ovarian cancer
Authors: Luo Weixuan , Liu Chunyue , Cheng Xiaoyan , Xu Wei , Zhou Wenjun , Ma Jiaxin , Ren Rui , Tian Meina , Ding Jing 
Published: February 2024

Abstract
Objective
Apatinib is a noval antiangiogenic agents, and it has great clinical benefit. Immunotherapy has a low response rate in patients with ovarian cancer, but combination with antiangiogenic therapy might improve the antitumour response. The aim of our study was to assess the expression of programmed cell death 1 ligand 1 (PD-L1) in vivo and in vitro and the potential effect of apatinib in combination with an anti-PD-1 antibody in mice with ovarian cancer.
Methods
We assessed the expression of PD-L1 in vivo and in vitro by real-time quantitative reverse transcription–polymerase chain reaction (qRT‒PCR), flow cytometry and western blotting in different concentrations. We constructed a xenograft model and measured mouse weight and tumour size after treatment. Tumour tissues from the mice were subjected to HE staining, and we detected Ki-67, CD4 and CD8 expression by immunohistochemical staining (IHC).
Results
Apatinib monotherapy increased the expression of PD-L1 by dose-dependent, as determined by qRT‒PCR, flow cytometry, and western blotting in vivo and in vitro. Apatinib combined with an anti-PD-1 antibody had the most pronounced antitumour effect on ovarian cancer among the combination treatment and the two monotherapies. The most obvious changes were a decrease in Ki-67 expression and increases in CD4 and CD8 expression.
Conclusions
Our study showed that apatinib upregulates the expression of PD-L1 to exert antitumour effects. Apatinib in combination with an anti-PD-1 antibody led to a remarkable reduction in tumour growth, providing evidence for clinical trials.
Review of research on performance indicators for water utilities
Authors: Sakai Hiroshi 
Published: February 2024

Abstract

Water utilities are affected by various social, environmental, and technological factors and are increasingly required to enhance their infrastructure and long-term efficiency. Performance indicators are useful tools for assessing the operational, financial, environmental, and social aspects of water systems. Given this background, the author reviewed the literature on performance indicators for the water sector and summarized the research trends as follows. As a perspective, there are a lot of good mathematical and theoretical studies on distribution pipes and leakage management. Future research should address the problems of water utilities, by using multiple levels of performance indicators including social and environmental context in the long term. Asset management and utility management studies address diverse and current problems faced by water utilities. However, there is still room for improvement in standardizing the methodology for data collection, processing, and integration. In addition, it is recommended for future research, to include carbon neutrality aspect, to include pipeline materials and soil information in leakage management, to extend asset management studies to treatment plants, with including additional indicators about human and financial resources.
Utilization of water hyacinth (Eichhornia crassipes) biomass as eco-friendly sorbent for petroleum oil spill cleanup
Authors: Khondoker Marufa , Gurav Ranjit , Hwang Sangchul 
Published: February 2024

Abstract

This study focuses on the removal of spent engine oil (SEO) spill from the water surface using water hyacinth biomass (WHB)-based sorbents. The raw WHB was modified using extra virgin coconut oil (mainly consisting of lauric acid) to enhance the hydrophobicity and floating ability. With varying amounts of coconut oil and solvent, six diverse types of modified water hyacinth biomass (MWHB) were prepared. Among these MWHBs, an equal proportion of coconut oil and raw WHB with 10% methanol solution exhibited the highest removal of SEO reaching 96%. Various sorption kinetics and isotherm models were examined to understand the SEO sorption process on MWHB. The pseudo-second-order kinetics (R2 0.999) and both the Langmuir and Freundlich isotherm models (R2 0.992 and R2 0.999, respectively) were found to be the best-fitting models. These findings indicated a chemisorption mechanism involving the initial monolayer coverage of SEO molecules on the MWHB surface followed by the development of multilayers. The MWHB achieved a maximum sorption capacity of 4.75 g/g within 60 min. Furthermore, the reusability tests showed that MWHB maintained a sorption capacity of over 90% even after the third sorption–desorption cycle.
Therapeutical Approach to Arterial Hypertension - Current State of
the Art
Authors: Radosavljevic Milica , Vučević Danijela , Samardžić Janko , Radenkovic Miroslav , Radosavljević Tatjana 
Published: February 2024

Abstract:
Arterial hypertension (AH) is recognized as the most common illness within
the group of cardiovascular diseases and the most massive chronic non-infectious disease
in the world. The number of hypertensive patients worldwide has reached 1.28 billion,
contributing to an increase in cardiovascular diseases and premature death globally.
The high prevalence of hypertension emphasizes the importance of effectively treating
this condition. Elevated blood pressure often leads to lethal complications (heart failure,
stroke, renal disorders, etc.) if left untreated. Considering an increase in AH prevalence
in the future, a successful therapeutical approach to this disease and its complications is
essential. The goal of AH treatment is to maintain normotensive blood pressure through
various approaches, including lifestyle changes, a well-balanced diet, increased physical
activity, psychoeducation, and, when necessary, pharmacotherapy. The evolving pharmacotherapeutic
landscape reflects the progress made in our understanding of hypertension
and emphasizes the need for continuous innovation to meet the challenges posed by this
prevalent global health concern. The journey toward more effective and tailored treatments
for hypertension is ongoing, and the introduction of new medications plays a pivotal
role in shaping the future of antihypertensive pharmacotherapy.
The Transcriptome Analysis of Circular RNAs Between the Doxorubicin-
Induced Cardiomyocytes and Bone Marrow Mesenchymal Stem Cells-
Derived Exosomes Treated Ones
Authors: Wei Yanhuan , Wei Haixia , Tian Chao , Wu Qinchao , Li Daisong , Huang Chao , Zhang Guoliang , Chen Ruolan , Wang Ni , Li Yonghong , Li Bing , Chu Xian-Ming 
Published: February 2024

Aim:
To analyze the sequencing results of circular RNAs (circRNAs) in cardiomyocytes
between the doxorubicin (DOX)-injured group and exosomes treatment group. Moreover,
to offer potential circRNAs possibly secreted by exosomes mediating the therapeutic effect on
DOX-induced cardiotoxicity for further study.

Methods:
The DOX-injured group (DOX group) of cardiomyocytes was treated with DOX,
while an exosomes-treated group of injured cardiomyocytes were cocultured with bone marrow
mesenchymal stem cells (BMSC)-derived exosomes (BEC group). The high-throughput sequencing
of circRNAs was conducted after the extraction of RNA from cardiomyocytes. The differential
expression of circRNA was analyzed after identifying the number, expression, and conservative
of circRNAs. Then, the target genes of differentially expressed circRNAs were predicted
based on the targetscan and Miranda database. Next, the GO and KEGG enrichment analyses
of target genes of circRNAs were performed. The crucial signaling pathways participating in the
therapeutic process were identified. Finally, a real-time quantitative polymerase chain reaction
experiment was conducted to verify the results obtained by sequencing.

Results:
Thirty-two circRNAs are differentially expressed between the two groups, of which
twenty-three circRNAs were elevated in the exosomes-treated group (BEC group). The GO
analysis shows that target genes of differentially expressed circRNAs are mainly enriched in the
intracellular signalactivity, regulation of nucleic acid-templated transcription, Golgi-related activity,
and GTPase activator activity. The KEGG analysis displays that they were involved in the
autophagy biological process and NOD-like receptor signaling pathway. The verification experiment
suggested that mmu_circ_0000425 (ID: 116324210) was both decreased in the DOX
group and elevated in BEC group, which was consistent with the result of sequencing.

Conclusion:
mmu_circ_0000425 in exosomes derived from bone marrow mesenchymal stem
cells (BMSC) may have a therapeutic role in alleviating doxorubicin-induced cardiotoxicity
(DIC).
Research Progress in Single-herb Chinese Medicine and Compound
Medicine for Knee Osteoarthritis
Authors: Zhou Guanghui , Zhang Xianquan , Gu Zhuoxu , Zhao Jinlong , Luo Minghui 
Published: February 2024

Abstract:
Due to an increase in the aging population, osteoarthritis (OA), especially knee osteoarthritis
(KOA), has increasingly become one of the diseases affecting the quality of life of the
elderly. As the pathogenesis of KOA is still unclear, Western medicine treatment lacks specificity,
and surgical treatment is difficult to cover all patients. Therefore, in recent years, traditional Chinese
medicine (TCM) for the conservative treatment of KOA has received increasing attention.
The advantages of TCM are clear, such as relief of symptoms, fewer adverse reactions, and wider
applicability to patients. This paper mainly discusses the research progress in single-herb TCM
and TCM compounds for KOA, aiming to demonstrate the effectiveness of TCM in the treatment
of KOA. It also provides ideas for future research on the treatment of KOA by TCM and the
pathogenesis of knee osteoarthritis.
Thiourea-Ammonium Chloride Mediated Knoevenagel Condensation as
an Intermediate in the Synthesis of Pyrimidine Scaffolds under
Solvent-free Condition
Authors: Rayate Ankita M. , Gaware Manoj R. 
Published: February 2024

Abstract:
In this paper, we have reported solvent solvent-free method for Knoevenagel condensation
reaction of various aldehydes with active methylene compounds using thiourea and ammonium chloride.
The developed method demonstrated high efficiency in the formation of C-C bond. The reaction
proceeds smoothly under mild and solvent-free conditions and the products obtained are in excellent
yield in very short duration. This method is applicable to a wide range of aldehydes with active
methylene compounds.
A Complete Overview of the Polycystic Ovarian Syndrome with Recent Advancement in Clinical Trial
Authors: Mukherjee Swarupananda , Rai Deepti , Ghoshal Debjit , Chakraborty Tania , Karati Dipanjan 
Published: February 2024

Background:
Polycystic ovarian syndrome (PCOS) has emerged as one of the most
common endocrine and metabolic disorders seen in women of childbearing age throughout the
whole world. The complex pathophysiology, different diagnostic criteria, and various manifestations
attached to several environmental factors, including lifestyle influences, have made it one of
the most difficult disorders to treat in recent times. In addition, inadequate knowledge among patients
and a lack of dedicated approved medications have only enhanced the difficulties in treating
such a heterogeneous disorder.

Objective:
The main objective of this review-type paper is to provide a detailed overview of
PCOS along with the current concept of a clinical stance in this complex multigenic disorder.

Method:
The following databases were used for literature searches: PubMed, Frontiers, Science
Direct, Springer, Wiley, and MDPI. For the purpose of finding pertinent articles and contents, the
keywords “PCOS; hirsutism; psychological burden; obesity” and others of a similar nature were
utilized.

Conclusion:
PCOS is a complicated hormonal, metabolic, and psychological condition with many
different clinical manifestations. It is among the most prevalent causes of infertility. Before considering
any medication choices, lifestyle modifications should be considered the primary therapeutic
prescription for PCOS-related infertility. According to recent studies, PCOS does not affect the
risk of ovarian or breast cancer, but it does raise the risk of endometrial cancer in women of all
ages. These results suggest that PCOS may increase the risk of gynaecological cancer morbidity.
The following stage is ovulation stimulation, which is best accomplished with letrozole and is followed
by clomiphene citrate. Women who had not responded to the first-line oral ovulatory
medicine were given gonadotropins as a backup. Early detection of girls with a high propensity to
develop PCOS will be made possible by a comprehensive knowledge of the condition's etiology.
Adolescent PCOS will be better managed overall, related comorbidities will be prevented, and
quality of life will increase with customized therapeutic approaches.
Anti Urolithiatic and Diuretic Potentiality of Hemidesmus indicus R. Br.
Authors: Das Saumya , Mazumder Avijit , Ojha Smriti , Pathak Deepika , Singh Himanshu , Pandey Pratibha , Pal Rashmi Saxena 
Published: February 2024

Aim:
The present study investigated the development of kidney stone formation in animal models involving renal tubular stone formation by ethylene glycol and COX-2 selective inhibitor-induced urolithiasis along with the diuretic potentiality by Lipschitz teston Wistar rats.

Background:
Hemidesmus indicus (H. indicus) R. Br. played a prominent role in various ancient
traditional systems of medications and possessed various pharmacological applications. Since the
last few decades, urolithiasis has been a major constraint in both livestock and human health.
Celecoxib administration increased urinary enzyme excretion but did not affect oxalate or citrate
excretion in a urolithiasis model.

Objective:
This research provides a comprehensive account of the ethnobotanical use of H. indicus as an antiurolithiatic and diuretic agent in animal models.

Methods:
The plant material was dried, pulverized into a dry powder, extracted with ethanol, and
analyzed for the presence of various secondary metabolites. The anti-urolithic effect of ethanolic
extract of H. indicus roots in albino rats was investigated using ethylene glycol (0.75%) and COX
-2 selective inhibitor models

Results:
The experimental data showed the significant effect of H. Indicus root extract (HIEE) as
anti-urolithiasis by the prevention of kidney stone formation, also by decreasing crystal nucleation, growth inhibition, decreased aggregation, and crystal retention within the renal tubules. The
effect of HIEE supplementation prevents the impairment of renal stone formation, which was also
confirmed by the histological findings. HIEE also acts as a potent diuretic, which supports the
study.

Conclusion:
The results indicated that HIEE was effective against experimentally induced urolithiasis, and it also acts as a potent diuretic in treated animals. So, it needs to perform future research on medicinal plants, including in vivo mechanistic and human studies for urolithiasis.
Effect of Pioglitazone and Cetuximab on Colon Cancer Stem-like Cell (CCSLCs) Properties
Authors: Alamdar Nasim , Farivar Shirin , Baghaei Kaveh , Hamidieh Amir Ali , Soltaninejad Hossein , Aghdaei Hamid Asadzadeh , Zali Mohammad Reza , Saltanatpour Zohreh 
Published: February 2024

Background:
One of the main reasons for cancer resistance to chemotherapy is the
presence of cancer stem cells (CSCs) in cancer tissues. It is also believed that CSCs are the unique
originators of all tumor cells. On the other hand, the Epithelial-Mesenchymal Transition pathway
(EMT) can act as the main agent of metastasis. Therefore, it is possible that targeting CSCs as
well as the EMT pathway could help in cancer therapy. Considering that CSCs constitute only a
small percentage of the total tumor mass, enrichment before study is necessary. In our previous
study, CSCs were enriched in the human colon cancer cell line HT29 by induction of EMT. These
CSC-enriched HT29 cells with mesenchymal morphology were named “HT29-shE”. In the present
study, these cells were used to investigate the effect of pioglitazone (Pio) and Cetuximab
(Cet) in order to find CSC and EMT targeting agents.

Methods:
The viability and IC50 rate of cells treated with different concentrations of Pio and Cet
were evaluated using the MTT test. EMT and CSC markers and cell morphology were assessed in
Pio and Cet treated and untreated HT29-shE cells using flow cytometry, realtime PCR, immunocytochemistry,
and microscopic monitoring.

Results:
The findings showed that Pio and Cet at concentrations of 250 μM and 40 μg/ml, respectively,
decrease cell viability by 50%. Also, they were able to reduce the expression of CSC markers
(CD133 and CD44) in the CSC enriched HT29 cell line. Furthermore, Pio and Cet could efficiently
reduce the expression of vimentin as a mesenchymal marker and significantly upregulate
the expression of E-cadherin as an epidermal marker of EMT and its reverse mesenchymal- toepithelial
transition (MET). In addition, the mesenchymal morphology of HT29-shE changed into
epithelial morphology after Cet treatment.

Conclusion:
Pio and Cet could inhibit EMT and reduce CSC markers in the EMT induced/CSC
enriched cell line. We expect that focus on finding EMT/CSC-targeting agents like these drugs
can be helpful for cancer treatment.