bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract TMP77: Robotic-Guided Implantation of 3D-Printed Hydrogel Scaffolds to Enhance Axonal Regeneration and Functional Recovery After Stroke: A Novel Minimally Invasive Surgical Approach
Authors: Nouduri Sirisha , Liang Lucy , Chalivoy Lucas , Quispe Johan , Ibrahim Souha , Oulmas Ali , Andre Arthur , François Quentin , Duplat Bertrand , Koffler Kobi , Pirondini Elvira , Gonzalez-Martinez Jorge 
Published: January 2025

Ischemic strokes often disrupt white matter (WM) microstructure, particularly affecting the corticospinal tract (CST) in the internal capsule (IC), leading to significant motor deficits. While evidence shows that axon sprouting occurs post-stroke, it often follows erratic paths, limiting functional recovery. Recent advances in 3D-printed hydrogel scaffolds have shown promise in guiding axon regeneration in spinal cord injury models. We hypothesize that implanting these scaffolds at white matter sites affected by stroke, aligned with CST fibers, will enhance functional axonal regeneration and motor recovery. Our aim is to develop and validate a precise and clinically applicable scaffold delivery system for future human studies.
We have developed a novel, minimally invasive stereotaxic method for the precise deployment of 1.3 mm diameter scaffolds within the IC of non-human primates (NHPs). Our approach utilizes a clinically available stereotactic robot (ROSA, ZimmerBiomet) combined with a linear cannula system (AlphaOmega) to plan scaffold placement. Then, we attempted to deploy scaffolds devices into thermally coagulated lesions in NHPs (n=2) and simulated lesions within hydrogel phantoms (n=2). Post-implant MRI, co-registered with high-resolution diffusion tensor imaging were applied to check for scaffold placement accuracy (n=4). We further complement the scaffold placement method by using a novel neurosurgical microrobot (Robeauté) designed for navigation along 3D nonlinear curves, with accuracy confirmed by CBCT imaging in phantom tests.
Our implantation methods accurately placed scaffolds in the intended locations and orientations, aligning with CST fibers in both phantom models and NHP brains. The deviation angle between the scaffold and CST fibers was 17.83° (SD 6.71°, n=3). The scaffolds were precisely positioned within the IC, validating our approach. Our robot-guided method accurately steered them to their intended position, demonstrating minimal errors in targeted white matter regions.
The methods described are poised for clinical translation, potentially extending the applicability of scaffold technology for improving motor function post-stroke. We are preparing NHPs for behavioral and upper limb motor assessments to evaluate the impact of scaffold implantation on axon regeneration, recovery, and performance. This research aims to enhance our understanding of ischemic stroke and develop innovative therapies to restore motor function in patients.
Abstract TP124: Perception of health status in stroke patients through Patient-Reported Outcome Measures depending on who collects them
Authors: Guirao Cristina , Rizzo Federica , Sanchez Ester , Paredes Carolina Kina , Baladas Maria , Montiel Estefania , Cano David , Ribo Marc , Molina Carlos , Rubiera Marta 
Published: January 2025

Value-based medicine places the patient and their health status at the center of the intervention through the use of Patient-Reported Outcome Measures (PROMs). The ideal would be that these outcome measurements were answered directly by the patient but in many cases it is a caregiver or a healthcare professional who collects the person's health status perception. This reason could lead to a bias in the results.
Our aim was to compare whether there were differences in the perception of health status depending on who answered these questionnaires.
Stroke patients discharged from six European hospitals were included in a 1-year follow-up program based on a holistic communication tool (web platform for professionals and app for patients/caregivers) called NORA. PROMs at 7-90 days were collected through NORA-app. In case that the patient or caregiver didn't have access to a smartphone, the data collection was carried out by a professional healthcare who contacted them to manage PROMs by a phone call.
Main outcome measures include: HAD-depression and HAD-anxiety (defined as pathological by a score ≥10 points in each of the subscales) and PROMIS-10 (cut-offs raws values of normality were defined as: Physical-PROMIS>13 and Mental-PROMIS>11). Median scores per collector were compared. In addition, a social questionnaire was collected from app-users'.
Over two years, 5116 stroke patients were included in Harmonics project, 60% were men with a mean age of 70.2 years and median mRS of 2(1- 3) at hospital discharge. From them, 2432 were actively monitored and 1498 reported PROMs (428 patients (28.6%), 376 (25.1%) caregivers and 694 (46.3%) professionals). P-value < 0.05 was considered significant for all tests at 90 days. Median PROMs results are shown in Table-1.
The social questionnaire (Figure-1) showed significant differences between male and female patients. From the total, 26.6% women and 11.7% men leave alone (p-value = 0.005).
At the patients group 77.9% women considered they can take care of their basic needs’ vs 85.9% men (p-value= 0.036).
Significant differences were found between the three groups of collectors, with professionals being the ones who perceive a better state of patient health through the collected PROMs collected. Among patients and caregivers groups, worse outcomes were reported by the last one.
When using PROMs the collector should avoid bias in reporting the results and direct patient response should be encouraged.
Abstract TP380: TMEM97 and Lipid Metabolism: Unraveling Post-Stroke Lipid Regulation
Authors: Arkelius Kajsa , Won Seok Joon , Gupta Lavanya , Zhang Mengqi , Swanson Raymond , SINGHAL NEEL 
Published: January 2025

Recent research on neurodegenerative diseases has highlighted a pathophysiological cascade characterized by abnormal lipid accumulation within the brain. Although progress has been made, the role of lipid metabolism following stroke remains unclear. This study aims to further explore changes in lipid metabolism after stroke, with a specific focus on the endoplasmic reticulum transmembrane protein 97 (TMEM97), which has emerged as a promising therapeutic target due to its critical role in cholesterol metabolism.
Spatial transcriptomic analyses following experimental stroke induction in mice revealed neuronal upregulation of crucial genes involved in lipid metabolism pathways, including LDLR, acetyl-CoA acetyltransferase 1, and elongation of very long-chain fatty acids protein 6, indicating dynamic adaptations post-stroke or under cellular stressors.
Initial findings demonstrate TMEM97 expression predominantly within neurons in vivo (mouse model). In an in vitro model of lipid starvation using mouse neural progenitor cells (MNPCs), pharmacological inhibition of lysosomal lipid release leads to increased expression of TMEM97. Interestingly, when TMEM97 was knocked down in MNPCs using lentiviral shRNA transfection, these cells exhibited no significant difference in neutral lipid accumulation compared to control cells. However, upon treatment with the lysosomal lipid release inhibitor, a significant increase in neutral lipid accumulation was observed exclusively in the TMEM97 knockdown cells.
Furthermore, Thapsigargin-induced endoplasmic reticulum (ER) stress in the MNPCs reduced TMEM97 expression, while also increasing low-density lipoprotein (LDL) uptake and the mRNA levels of the LDL receptor (LDLR). The observed elevation of neuronal LDLR expression in vitro closely aligns with the heightened expression of LDLR in vivo, suggesting a coordinated modulation of lipid metabolism pathways triggered by ER stress in response to stroke or metabolic stress induction. This synchronous upregulation of LDLR underscores the crucial role of ER stress as a potential trigger for this regulatory response. Additionally, considering the known involvement of TMEM97 in lipid homeostasis within the ER, it further highlights the intricate interplay between these proteins in post-stroke lipid metabolism regulation.
Our findings provide a compelling starting point for further exploring TMEM97's significance in post-ischemic lipid homeostasis.
Abstract TP397: Effect of Intracranial Pressure Spikes Modeling Subarachnoid Aneurysm Rupture on the Brain and Dura Barrier Immune System
Authors: Mattke Jordan , Shah Vaidehi , Mastorakos Panagiotis 
Published: January 2025

Steep intracranial pressure (ICP) rise constitutes a hallmark of aneurysmal subarachnoid hemorrhage (SAH). While during aneurysm rupture there are multiple simultaneous triggers that can initiate an inflammatory response, in this study we isolate the impact of a single ICP spike on the brain and meningeal barriers and the resulting secondary injury.
Using a rodent model, we induced an ICP spike through cisterna magna injection of artificial cerebrospinal fluid or autologous blood, with simultaneous ICP monitoring. We then assessed the innate immune response in the brain parenchyma and dura using immunohistochemistry and multiparameter flow cytometry. Glia limitans integrity was evaluated via intrathecal injection of the SR101 tracer, and microthrombi presence was assessed using intravenous fluorescent fibrin. Upregulated pathways were identified through bulk RNA sequencing.
Our findings revealed that a single ICP spike exerts global effects on brain and meningeal barrier immunology, potentially leading to secondary injury. Histological analysis and flow cytometry of dura whole mounts showed extensive cell death of resident CD206+ macrophages, essential for steady-state immune surveillance, alongside infiltration of Ly6C+ pro-inflammatory monocytes and Ly6G+ neutrophils, indicating a robust acute response to the ICP spike. In the brain parenchyma, we observed minimal infiltration of circulating immune cells, consistent with the effects of aneurysm rupture. However, diffuse microglia clustering suggested multifocal parenchymal injury. Additionally, there was evidence of widespread glia limitans disruption and microthrombi formation in the brain parenchyma. Bulk RNA sequencing supported these findings, demonstrating upregulation of MyD88 and TNFα pathways in the dura, which were absent in the brain.
In conclusion, this study demonstrates that a single ICP spike triggers significant meningeal inflammation, disrupts steady-state dura immune surveillance, and causes diffuse parenchymal injury with glia limitans disruption. These results highlight a novel blood-independent pathway of injury following aneurysm rupture, offering new insights into potential therapeutic targets.
Detecting glioblastoma infiltration beyond conventional imaging
tumour margins using MTE-NODDI
Authors: Karamched Saketh R. , Gorup Dunja , Tolomeo Daniele , Brooks Lucy J. , Gong Ting , Jørgensen Andreas Christ Sølvsten , Hill Ciaran Scott , Clements Melanie , Kalber Tammy L. , Wells Jack A. , Stuckey Daniel J. , Thorne Lewis , Shahrezaei Vahid , Marguerat Samuel , Zhang Hui , Parrinello Simona , Lythgoe Mark F. 
Published: January 2025

Abstract
Glioblastoma (GBM) is the most common and aggressive brain tumour with stark resistance to available therapies, leading to relapse and a median survival of &lt;15 months. A key cause of therapy resistance is diffuse infiltration of tumour cells into brain regions surrounding the tumour, which presents a major clinical challenge as existing imaging techniques offer limited detection of the resectable margin. Here, we use diffusion weighted imaging (DWI) and apply the multiple echo time neurite orientation dispersion and density imaging (MTE-NODDI) model as a tool to detect tumour cells in the hard-to-distinguish margin. We used the G144 patient-derived xenograft model, with characteristic invasion along white matter tracts, in combination with MTE-NODDI. Tumour development was monitored, and magnetic resonance imaging (MRI) data were acquired over a 4-week period, starting at 4 weeks after stereotactic injection of tumour cells. MTE-NODDI demonstrated sensitivity to the developing tumour in the invading margin, and changes in measured parameters were apparent from 6 weeks after injection. In comparison to standard DWI, MTE-NODDI showed increased sensitivity to the tumour-associated changes in the margin. Furthermore, extraneurite volume fraction (fen) and neurite density index (NDI) measured from MTE-NODDI correlated with immunohistological measurement of tumour cells. These findings suggest that MTE-NODDI may non-invasively detect infiltrating cells and tumour-induced pathology in margin regions without T2 or DWI changes in a patient-derived mouse model of GBM. MTE-NODDI is clinically translatable and could be a powerful tool for neurosurgeons to maximise surgical resection, resulting in better survival outcomes for patients with GBM.
Fast-Forwarding the Future: Takarazuka Revue’s Recalibration of Youth Cultures
Authors: Grajdian Maria 
Published: January 2025

Abstract
Within a mixed-methodological framework, this paper analyzes Takarazuka Revue’s groundbreaking performances City Hunter: The Stolen xyz (2021, snow troupe) and High & Low: The Prequel (2022, cosmos troupe): famously rigid in its business model targeted at a clearly defined socioeconomic demographic (upper-middle and upper class female citizens aged from 35 up to 80s or even 90s), these two performances manifest a subtle paradigm shift in Takarazuka Revue’s performance strategy towards a consideration of younger audiences of both genders through the employment of a diverse narrative and musical material and the immersion into the problematic of global concerns such as family dysfunctionality, rampant loneliness and loss of valid role-models.
Energy gain kernel for climate feedbacks. Part II: Spatial pattern of surface amplification factor and its dependency on climate mean state
Authors: Hu Xiaoming , Cai Ming , Sun Jie , Ding Feng , Feng Jing 
Published: January 2025

Abstract
In this study, we examine the spatial pattern of the surface amplification factor (SAF). SAF corresponds to the surface element of the energy gain kernel derived in Part I of this three-part series papers, representing the amplification rate of input energy perturbations at the surface. At a given location, SAF amplifies surface energy perturbations at an equal rate regardless of their origins. Therefore, the spatial variation of SAF can provide insight into the spatial variability of climate sensitivity. The global mean of SAF is about 2.6, and its spatial pattern closely resembles the climate mean cloud field. SAF values range from 4 over the western equatorial Pacific, 2.8-3.2 over mid-latitude storm track regions, and 2.0-2.8 over the Arctic, to 1.4-1.7 over the Antarctic.
The more longwave (LW) absorbers in the atmosphere, the greater SAF is. Therefore, SAF is greater in regions where atmospheric water vapor is abundant such as the tropics, and where clouds are prevalent such as mid-latitude storm tracks and the Arctic, but have smaller values close to unity in cold, high-elevation regions. Moreover, SAF tends to negatively correlate with surface temperature when LW absorbers are abundant and positively when they are scarce. With limited LW absorbers, SAF exhibits a negative (positive) correlation in cold (warm) conditions. While the spatial pattern of surface temperature plays a secondary role in shaping SAF, it may help explain why the climate sensitivity in models can either increase or decrease with surface temperature depending on the LW absorbers in the atmosphere.
Domain-Specific Memory Impairments in Bipolar Mania: Insights from a Tertiary Care Hospital
Authors: Kumar Mohit , Kumar Sanjay , Khusboo  , Maqbool Masood , Singh Vinit Kumar , Soni Amit Kumar 
Published: January 2025

Background: Cognitive deficits in bipolar affective disorder (BPAD), particularly during manic episodes, are well-documented. However, research on domain-specific memory impairments in bipolar mania is limited, especially in the Indian subcontinent. This study aimed to assess memory impairments in individuals with bipolar disorder using the Postgraduate Institute Memory (PGI-Memory) scale and to highlight domain-specific deficits compared to healthy controls. Methods: This cross-sectional study was conducted at a tertiary care center in North India. Twenty subjects diagnosed with bipolar mania and 20 age, sex, and education-matched healthy controls between the ages of 18 and 40 were recruited. Memory functions were assessed using the PGI-Memory scale. Mental control and working memory were also evaluated. Results: Both groups were matched in terms of age, sex, and education. Individuals with bipolar disorder demonstrated significant deficits in various memory domains, including immediate ( d = 1.47), recent ( d = 0.93), remote ( d = 1.58), long-term ( d = 2.37), and associative memory (similar pairs: d = 1.4, dissimilar pairs: d = 1.84,), as well as in visual reproduction ( d = 2.3) and recognition tasks ( d = 1.54). In contrast, their working memory performance was comparable to that of the control group. The largest deficits were observed in long-term memory, visual reproduction, and associative memory. Conclusions: Bipolar mania is associated with widespread memory impairments, particularly in long-term and associative memory, which may contribute to difficulties in emotional regulation and daily functioning. These findings emphasize the importance of considering memory impairments in the diagnosis and management of BPAD. Further studies are required to investigate the neurobiological foundations of these impairments and to develop specific interventions.
Mapping Interdisciplinary Role Ownership Over Actionable Practices Identified From the Bereaved Family Survey
Authors: Chow Janice Kishi , McCaa Matthew , Bozkurt Selen , Gamboa Raziel Cecilia , Lorenz Karl A. , Giannitrapani Karleen F. 
Published: January 2025

Purpose To determine the feasibility of mapping interdisciplinary role ownership over actionable practices identified from qualitative comments in the Veterans Affairs Bereaved Family Survey (BFS). Methods We polled two providers from each of 14 disciplines as to whether an actionable practice that improved end-of-life care quality sits within their scope of practice. We grouped practices by having the greatest, middle, and fewest number of disciplines that claimed role ownership and then characterized what roles were shared. Major Findings Medicine, nursing, social work, and occupational therapy claimed role ownership of the greatest number of practices among the 14 disciplines. Chaplaincy and the allied health disciplines had a comparatively more limited range of role ownership of practices. Practices with the greatest number of professionals claiming role ownership were general professional behaviors that do not require specialized training (e.g., being easy to talk to). Practices with the middle number of role ownership required clinical specialization (e.g., offering non-pharmacological interventions to reduce agitation). Practices with the fewest number of disciplines required a narrower skill set (e.g., signing the death certificate in a timely manner) or administrative authority to perform (e.g., providing adequate nursing staff). Conclusions This project demonstrated the feasibility of mapping BFS actionable practices across disciplines. A larger sample and rigorous statistical analysis are required for application at a health care system level. Understanding role ownership can then guide efforts relating to role ownership and role sharing for end-of-life quality improvement activities.
Self-rated health in preadolescent children as a predictor for hospital contacts and redeemed prescriptions: the Danish National Birth Cohort 1996–2002
Authors: Renneberg Camilla Klinge , Rask Charlotte Ulrikka , Bernstorff Martin , Rytter Dorte , Hansen Stefan Nygaard , Bech Bodil Hammer 
Published: January 2025

Aim: The decline in self-rated health among young people raises concerns for future health outcomes. We examined whether self-rated health in preadolescence predicts hospital contacts and prescription redemption in later adolescence. Methods: This longitudinal study uses the Danish National Birth Cohort on children born 1996–2002. A questionnaire was distributed to 96,382 11-year-old preadolescents followed from 2010 to 2018. Number of hospital contacts and prescriptions were obtained from Danish registers. Incidence rate ratios (IRRs) were estimated, comparing preadolescents with poor (‘Not So Good’/’Poor’) and ‘Good’ self-rated health to those with ‘Excellent’ self-rated health. Results: Among the 47,365 (49.1%) 11-year-old participants, 5.3% reported poor self-rated health and 38.3% reported good health. Analyses revealed that children with good self-rated health had higher rates of somatic (IRR, 1.17 [1.14–1.21]) and psychiatric (IRR, 1.63 [1.47–1.81]) hospital contacts. Children with poor self-rated health showed even higher rates, with IRRs of 1.73 [1.62–1.85] for somatic and 3.54 [3.03–4.15] for psychiatric contacts. Additionally, those with good self-rated health had more somatic and psychiatric prescriptions (IRR, 1.25 [1.21–1.30] and 1.75 [1.55–1.97], respectively), whereas children with poor self-rated health had IRRs of 1.67 [1.56–1.80] for somatic and 4.03 [3.39–4.82] for psychiatric prescriptions. Conclusion: Preadolescents with both good and poor self-rated health show higher rates of hospital contacts and prescription redemption in adolescence, especially pronounced in children with poor self-rated health. This indicates that early-life health perceptions impact long-term wellbeing, particularly psychiatric health. This simple self-rated health measure provides valuable insights for healthcare professionals by aiding in identifying children in need of support and early intervention.
Using ethnomethodology to explore how nurses, in an acute setting, make sense of care
Authors: Healy Ray 
Published: January 2025

Aim: To explore how nurses understand and make sense of multiple definitions of nursing practice. Methodology: The study used ethnomethodology to explore how nurses in an acute hospital ward shape, construct and sensemake nursing practice in their everyday worlds. Multiple data sources were used to triangulate different constructions of reality and generate a broader understanding, including non-participant shadowing of nurses during eight shifts, semi-structured interviews with six registered nurses and a review of the nursing section of the hospital’s Electronic Patient Record system. Data were collected in two surgical wards in an acute hospital (2019–2020). Data were iteratively coded and refined through all stages of the study. Findings: Nurses make sense of conflicting expectations by creating multiple realities which they apply in different situations to structure how they deliver care. This finding suggests that nurses move seamlessly and unknowingly through these created realities, supported by using specific but discrete languages that they can effortlessly adopt. A consequence is that no single model or theory contains all the realities. Conclusion: This study goes some way to explain the described difference between work-as-imagined and work-as-done. Failure to acknowledge the multiple realities constructed in practice during pre-registration education may explain the theory–practice gap that many new graduates experience and why anticipated outcomes described in research projects might not be realised in everyday practice. Implications: Nursing practice is not governed by a single theory or model, and nurses make pragmatic transitions between different expectations. Recognition of this is critical to effective planning and leadership of the nursing resource. Using electronic records as a single measure of nursing work has the potential to create a bias towards one reality and thereby render other aspects of nursing value invisible. Failure to embrace the totality of nursing practice may impede the delivery of anticipated patient outcomes and system efficiencies.