bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural diversity of heat-induced transcription of retrotransposons inArabidopsis thaliana
Authors: Xu Wenbo , Thieme Michael , Roulin Anne C. 
Published: January 2024

AbstractTransposable elements (TEs) are major components of plant genomes, profoundly impacting the fitness of their hosts. However, technical bottlenecks have long hindered our mechanistic understanding of TEs. Using RNA-Seq and long-read sequencing with Oxford Nanopore Technologies’ direct cDNA sequencing, we analyzed the heat-induced transcription of TEs in three natural accessions ofArabidopsis thaliana(Cvi-0, Col-0, and Ler-1). In addition to the well- studiedONSENretrotransposon family, we identifiedCopia-35as a second heat-responsive retrotransposon family with particularly high activity in the relict accession Cvi-0. Our analysis revealed distinct expression patterns of individual TE copies and suggest different mechanisms regulating the GAG protein production in theONSENversusCopia-35families. In addition, analogously toONSEN,Copia-35activation led to the upregulation of flanking genes such asAMUP9and potentially to the quantitative modulation of flowering time. Unexpectedly, our results indicate that for both families, the upregulation of flanking genes is not directly initiated by transcription from their 3’ LTRs. These findings highlight the inter- and intraspecific expressional diversity linked to retrotransposon activation under stress, providing insights into their potential roles in plant adaptation and evolution at elevated temperatures.
Creation and Validation of the First Infinium DNA Methylation Array for the Human Imprintome
Authors: Carreras-Gallo Natalia , Dwaraka Varun B. , Jima Dereje D. , Skaar David A. , Mendez Tavis L. , Planchart Antonio , Zhou Wanding , Jirtle Randy L. , Smith Ryan , Hoyo Cathrine 
Published: January 2024

AbstractBackgroundDifferentially methylated imprint control regions (ICRs) regulate the monoallelic expression of imprinted genes. Their epigenetic dysregulation by environmental exposures throughout life results in the formation of common chronic diseases. Unfortunately, existing Infinium methylation arrays lack the ability to profile these regions adequately. Whole genome bisulfite sequencing (WGBS) is the unique method able to profile these regions, but it is very expensive and it requires not only a high coverage but it is also computationally intensive to assess those regions.FindingsTo address this deficiency, we developed a custom methylation array containing 22,819 probes. Among them, 9,757 probes map to 1,088 out of the 1,488 candidate ICRs recently described. To assess the performance of the array, we created matched samples processed with the Human Imprintome array and WGBS, which is the current standard method for assessing the methylation of the Human Imprintome. We compared the methylation levels from the shared CpG sites and obtained a mean R2= 0.569. We also created matched samples processed with the Human Imprintome array and the Infinium Methylation EPIC v2 array and obtained a mean R2= 0.796. Furthermore, replication experiments demonstrated high reliability (ICC: 0.799-0.945).ConclusionsOur custom array will be useful for replicable and accurate assessment, mechanistic insight, and targeted investigation of ICRs. This tool should accelerate the discovery of ICRs associated with a wide range of diseases and exposures, and advance our understanding of genomic imprinting and its relevance in development and disease formation throughout the life course.
RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection
Authors: Stamm Chelsea E. , McFarland Adelle P. , Locke Melissa N. , Tabakh Hannah , Tang Qing , Thomason Maureen K. , Woodward Joshua J. 
Published: January 2024

AbstractTransposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seqin vivois challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here we have developed a murine model with a disruption inAkr1c13that renders the resulting RECON-/-mouse resistant to high dose infection. We leveraged this model to perform a Tn-seq screen of the human pathogenListeria monocytogenes in vivo. We identified 139 genes which were required forL. monocytogenesgrowth in mice including novel genes not previously identified for host survival. We identified organ specific requirements forL. monocytogenessurvival and investigated the role of the folate enzyme FolD inL. monocytogenesliver pathogenesis. A mutant lackingfolDwas impaired for growth in murine livers by 2.5-log10compared to wildtype and failed to spread cell-to-cell in fibroblasts. In contrast, a mutant inalsR,which encodes a transcription factor that represses an operon involved in D-allose catabolism, was attenuated in both livers and spleens of mice by 4-log10and 3-log10, respectively, but showed modest phenotypes inin vitromodels. We confirmed that dysregulation of the D-allose catabolism operon is responsible for thein vivogrowth defect, as deletion of the operon in the ΔalsRbackground rescued virulence. By undertaking an unbiased, genome-wide screen in mice, we have identified novel fitness determinants forL. monocytogeneshost infection, which highlights the utility of the RECON-/-mouse model for future screening efforts.ImportanceListeria monocytogenesis the gram-positive bacterium responsible for the food-borne disease Listeriosis. Although infections withL. monocytogenesare limiting in healthy hosts, vulnerable populations, including pregnant and elderly people, can experience high rates of mortality. Thus, understanding the breadth of genetic requirements forL. monocytogenes in vivosurvival will present new opportunities for treatment and prevention of Listeriosis. We developed a murine model of infection using a RECON-/-mouse that is restrictive to systemicL. monocytogenesinfection. We utilized this model to screen forL. monocytogenesgenes requiredin vivovia Tn-seq. We identified the liver-specific genefolDand a repressor,alsRthat only exhibits anin vivogrowth defect. AlsR controls the expression of the D-allose operon which is a marker in diagnostic techniques to identify pathogenic Listeria. A better understanding of the role of the D-allose operon in human disease may further inform diagnostic and prevention measures.
New physics implications of vector boson fusion searches exemplified through the Georgi-Machacek model
Authors: Chakraborti Manimala , Das Dipankar , Ghosh Nivedita , Mukherjee Samadrita , Saha Ipsita 
Published: January 2024

LHC searches for nonstandard scalars in vector boson fusion (VBF) production processes can be particularly efficient in probing scalars belonging to triplet or higher multiplet representations of the Standard Model SU(2)L gauge group. They can be especially relevant for models where the additional scalars do not have any tree level couplings to the Standard Model fermions, rendering VBF as their primary production mode at the LHC. In this work we employ the latest LHC data from VBF resonance searches to constrain the properties of nonstandard scalars, taking the Georgi-Machacek model as a prototypical example. We take into account the theoretical constraints on the potential from unitarity and boundedness from below as well as indirect constraints coming from the signal strength measurements of the 125 GeV Higgs boson at the LHC. To facilitate the phenomenological analysis we advocate a convenient reparametrization of the trilinear couplings in the scalar potential. We derive simple correlations among the model parameters corresponding to the decoupling limit of the model. We explicitly demonstrate how a combination of theoretical and phenomenological constraints can push the Georgi-Machacek model towards the decoupling limit. Our analysis suggests that the VBF searches can provide key insights into the composition of the electroweak vacuum expectation value.

Published by the American Physical Society
2024
Spectral analysis for elastica dynamics in a shear flow
Authors: Liu Lujia , Sznajder Paweł , Ekiel-Jeżewska Maria L. 
Published: January 2024

We present the spectral analysis of three-dimensional dynamics of an elastic filament in a shear flow of a viscous fluid at a low Reynolds number in the absence of Brownian motion. The elastica model is used. The fiber initially is almost straight at an arbitrary orientation, with small perpendicular perturbations in the shear plane and out-of-plane. To analyze the stability of both perturbations, equations for the eigenvalues and eigenfunctions are derived and solved by the Chebyshev spectral collocation method. It is shown that their crucial features are the same as in the case of the two-dimensional elastica dynamics in shear flow [Becker and Shelley, ] and the three-dimensional elastica dynamics in the compressional flow [Chakrabarti , ]. We find a similar dependence of the buckled shapes on the ratio of bending to hydrodynamic forces as in the simulations for elastic fibers of a nonzero thickness [Słowicka , ].

Published by the American Physical Society
2024
Impact of information systems (IS) infusion on Open Government Data (OGD) adoption
Authors: Matheus Ricardo , Alexopoulos Charalampos , Rizun Nina , Loukis Euripides , Saxena Stuti 
Published: January 2024

Purpose
This study aims to underline the possible influence of the moderator, information systems (IS) infusion, on Open Government Data (OGD) adoption and usage.

Design/methodology/approach
Using the partial least squares-structural equation modeling methodological approach, the adapted unified theory of acceptance and use of technology (UTAUT) model has been used for understanding the role of the moderating variable, namely, IS infusion.

Findings
Findings show that the moderating impact of IS infusion is positively significant with respect to the performance expectancy–behavioral intention relationship thereby bolstering the impact on users’ perception of OGD vis-à-vis work/academics performance and negatively significant for social influence–behavioral intention, information quality–behavioral intention, thereby clinching the fact that with the increased engagement and involvement of OGD in the everyday life of the user, the role of significant others and information quality gets least significant.

Originality/value
Extant OGD-focused research has underscored the impact of different variables as far as OGD adoption and usage is concerned; the present study seeks to add on to the extant literature by understanding the implications of IS infusion on the adapted UTAUT model constructs and behavioral intention relationships.
TikTok as a lens into teacher attrition: perspectives from #teacherquittok
Authors: Barber Chelsey , Literat Ioana 
Published: January 2024

Purpose
A key social networking site for teachers, TikTok offers a new and valuable lens on educator attrition. This study aims to explore social media’s role in the increased transparency around leaving the profession and the online narratives crafted around transitioning out of the classroom.

Design/methodology/approach
Drawing on the conceptual framework of emergent storytelling and a recursive thematic analysis of videos and comments posted to the #teacherquittok hashtag on TikTok, this study examines how teachers are using social media to share their experiences of exiting the classroom.

Findings
The authors find that teachers used TikTok to share personal accounts that form a meta-narrative that provides context to their decisions to leave, share stories of loss and gain through negotiating the transition out of the classroom and finally debate the implications for preservice teachers. The authors discuss key takeaways for rethinking teacher support, teacher education and the role of social media in teachers’ professional lives.

Originality/value
While many studies seek to understand teacher attrition, this work examines how teachers’ stories shared on social media may be shaping attrition into an increasingly networked and narrated act.
Transforming first-aid training: a new lesson study approach for the Red Cross
Authors: Hanfstingl Barbara , Ogradnig Thomas Andreas 
Published: January 2024

PurposeThe first-aid courses organized by the Youth Red Cross Carinthia (Austria) had a quality problem, necessitating a professionalization in teaching and time structure. This research aimed to enhance the quality and effectiveness of these courses by implementing modified lesson studies with non-professional trainers. The paper presents the realization process, empirical research and results obtained by applying the first-aid curriculum.Design/methodology/approachAround 22 lesson study first-aid courses (14 classes with 2 cycles, 8 with 3 cycles) were conducted and evaluated in different Austrian school types. An observation sheet was created to evaluate attention and competencies. Interviews were conducted with both teachers and students to validate the results.FindingsThe research findings demonstrate that lesson studies can significantly enhance the quality and effectiveness of first-aid courses. Inexperienced and experienced first-aid teachers significantly improved their teaching skills. Newly educated first-aid teachers showed substantial improvement, leading to the introduction of an induction period and coaching opportunity within the Youth Red Cross Carinthia.Originality/valueThis is the first lesson study conducted in a non-academic context. It highlights the adaptation process of Carinthian first-aid courses. It illustrates how lesson studies impact lesson clarity, instructional variety, student engagement in the learning process, student outcome, student feedback and teaching effectiveness in a non-academic context. It contributes to the literature on the application of lesson study in first-aid education and provides insight into the benefits of this approach in enhancing the quality of first-aid training.
Optimal service resource management strategy for IoT-based health information system considering value co-creation of users
Authors: Fang Ji , Lee Vincent C.S. , Wang Haiyan 
Published: January 2024

PurposeThis paper explores optimal service resource management strategy, a continuous challenge for health information service to enhance service performance, optimise service resource utilisation and deliver interactive health information service.Design/methodology/approachAn adaptive optimal service resource management strategy was developed considering a value co-creation model in health information service with a focus on collaborative and interactive with users. The deep reinforcement learning algorithm was embedded in the Internet of Things (IoT)-based health information service system (I-HISS) to allocate service resources by controlling service provision and service adaptation based on user engagement behaviour. The simulation experiments were conducted to evaluate the significance of the proposed algorithm under different user reactions to the health information service.FindingsThe results indicate that the proposed service resource management strategy, considering user co-creation in the service delivery, process improved both the service provider’s business revenue and users' individual benefits.Practical implicationsThe findings may facilitate the design and implementation of health information services that can achieve a high user service experience with low service operation costs.Originality/valueThis study is amongst the first to propose a service resource management model in I-HISS, considering the value co-creation of the user in the service-dominant logic. The novel artificial intelligence algorithm is developed using the deep reinforcement learning method to learn the adaptive service resource management strategy. The results emphasise user engagement in the health information service process.
Breaking the e-waste stigma: how corporate gender diversity drives sustainable change in the UK
Authors: Issa Ayman , In’airat Mohammad 
Published: January 2024

Purpose
This study aims to investigate the relationship between female leaders at board and executive levels and e-waste reduction in firms listed on the FTSE All-Share Index.

Design/methodology/approach
The study uses a sample of nonfinancial firms listed in the FTSE All-Share Index between 2004 and 2021, comprising 2,523 firm observations. The primary technique used is ordinary least squares, with subsample analysis and the two-stage least squares method used to address endogeneity concerns.

Findings
This study suggests that the presence of female directors and executives can bring a more comprehensive and diverse approach to e-waste management, which can contribute to improved e-waste reduction initiatives. However, the study also highlights that the impact of female leadership on e-waste reduction may vary based on factors such as the size of the firm and the industry’s carbon footprint.

Practical implications
The practical implications of this research have noteworthy implications for companies and policymakers alike. By placing importance on gender diversity, companies can reap the benefits of diverse perspectives and approaches when addressing environmental challenges. Policymakers, on the other hand, can contribute to positive environmental outcomes by advocating for gender diversity in corporate leadership.

Originality/value
The novelty of this research stems from its discovery that having female directors and executives in a firm leads to a broader and more varied approach to managing e-waste, ultimately enhancing efforts to reduce it. This underscores the significance of gender diversity in advancing sustainable practices within organizations. The study highlights the distinct viewpoints and experiences that women offer when tackling environmental issues in the corporate sphere.
Forced shift to teleworking: how abusive supervision promotes counterproductive work behavior when employees experience COVID-19 corporate social responsibility
Authors: Liang Yuan , Wu Tung-Ju , Wang Yushu 
Published: January 2024

PurposeThe COVID-19 pandemic necessitated teleworking, which inadvertently led to an impaired communication between supervisors and employees, resulting in abusive supervision. Drawing on the conservation of resources (COR) theory and the social identity theory, this study aims to address this negative association by examining the mediating role of state mindfulness and the moderating role of COVID-19 corporate social responsibility (CSR) in the relationship between abusive supervision and counterproductive work behaviors.Design/methodology/approachThis research employs both qualitative and quantitative research designs. Data collection involved an experimental design with 117 participants (Study 1), a cross-sectional survey with 243 participants (Study 2) and semi-structured interviews with 24 full-time employees (Study 3).FindingsThe results reveal that state mindfulness acts as a mediator in the positive relationship between abusive supervision and counterproductive work behaviors (CWB). Furthermore, COVID-19 CSR mitigates the relationship between abusive supervision and CWB within the organization, but not with the supervisor. Additionally, COVID-19 CSR moderates the impact of abusive supervision on state mindfulness.Practical implicationsThe results emphasize the crucial role of CSR when employees encounter abusive supervision during the COVID-19 pandemic. Organizations and managers should adopt appropriate strategies to enhance employees' perception of CSR. Prioritizing the cultivation of state mindfulness is also recommended, and organizations can provide short-term mindfulness training to improve employees' state mindfulness.Originality/valueThis research contributes to the understanding of abusive supervision and CWB in the context of forced teleworking.
Microsoft Copilot and Anthropic Claude AI in education and library service
Authors: Adetayo Adebowale Jeremy , Aborisade Mariam Oyinda , Sanni Basheer Abiodun 
Published: January 2024

Purpose
This study aims to explore the collaborative potential of Microsoft Copilot and Anthropic Claude AI as an assistive technology in education and library services. The research delves into technical architectures and various use cases for both tools, proposing integration strategies within educational and library environments. The paper also addresses challenges such as algorithmic bias, hallucination and data rights.

Design/methodology/approach
The study used a literature review approach combined with the proposal of integration strategies across education and library settings.

Findings
The collaborative framework between Copilot and Claude AI offers a comprehensive solution for transforming education and library services. The study identifies the seamless combination of real-time internet access, information retrieval and advanced comprehension features as key findings. In addition, challenges such as algorithmic bias and data rights are addressed, emphasizing the need for responsible AI governance, transparency and continuous improvement.

Originality/value
Contribute to the field by exploring the unique collaborative framework of Copilot and Claude AI in a specific context, emphasizing responsible AI governance and addressing existing gaps.
Empowerment through engagement: assessing the female university students’ involvement in volunteering activities
Authors: Rubel Md. Ariful Islam , Rumi Maruf Hasan , Ripon Md. Abujafar 
Published: January 2024

PurposeThis study aims to identify how the involvement of female university students in volunteer organisations is contributing to their self-empowerment in Bangladesh.Design/methodology/approachA cross-sectional study with a qualitative approach was conducted. A total of 20 in-depth interviews with female student volunteers, two focus group discussions with student members of the volunteer organisation and ten key informant interviews with academics, gender experts and high-level representatives of civil society organisations were conducted. The data were collected from eight purposively selected universities in Dhaka. The data were collected between 1 January 2023 and 23 March 2023.FindingsThe inter-organisational environment appears to be supportive and welcoming, despite the fact that some male colleagues belittle women with insulting comments. Female students are predominantly involved in public presentation activities and their advice is rarely considered. To reach a top position, they have to show more commitment than their male colleagues. Subjective prejudices discourage women from taking on positions of responsibility. Participation in voluntary organisations strengthens the self-confidence, critical thinking, networking and social capital of female students, making it easier for them to gain employment. These factors contribute to the empowerment of female students.Originality/valueThis study is an excellent empirical document that establishes a link between the empowerment of female students and their participation in voluntary activities. The recommendations of this study pave the way for future research in different countries on how volunteering helps young people, especially female students, to shape their future by taking on social responsibility alongside their academic activities.