bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulses
Authors: Visser Diana , van Zuylen Gertan A. , van Dam Jan C. , Eman Michael R. , Pröll Angela , Ras Cor , Wu Liang , van Gulik Walter M. , Heijnen Joseph J. 
Published: September 2004

AbstractThis article presents the dynamic responses of several intra‐ and extracellular components of an aerobic, glucose‐limited chemostat culture of Saccharomyces cerevisiae to glucose and ethanol pulses within a time window of 75 sec. Even though the ethanol pulse cannot perturb the glycolytic pathway directly, a distinct response of the metabolites at the lower part of glycolysis was found. We suggest that this response is an indirect effect, caused by perturbation of the NAD/NADH ratio, which is a direct consequence of the conversion of ethanol into acetaldehyde. This effect of the NAD/NADH ratio on glycolysis might serve as an additional explanation for the observed decrease of 3PG, 2PG, and PEP during a glucose pulse. The responses measured during the ethanol pulse were used to evaluate the allosteric regulation of glycolysis. Our results confirm that FBP stimulates pyruvate kinase and suggest that this effect is pronounced. Furthermore, it appears that PEP does not play an important role in the allosteric regulation of phosphofructo kinase. © 2004 Wiley Periodicals, Inc.
Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles
Authors: Persson Patrik , Baybak Oxana , Plieva Fatima , Galaev Igor Y. , Mattiasson Bo , Nilsson Bernt , Axelsson Anders 
Published: September 2004

AbstractA continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. © 2004 Wiley Periodicals, Inc.
Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol
Authors: Besumbes Óscar , Sauret‐Güeto Susanna , Phillips Michael A. , Imperial Santiago , Rodríguez‐Concepción Manuel , Boronat Albert 
Published: September 2004

AbstractPaclitaxel (Taxol) is a widely used anticancer isoprenoid produced by the secondary metabolism of yew (Taxus sp.) trees. However, only limited amounts of Taxol or related metabolites (taxoids) can be obtained from the currently available sources. In this work we have taken the first step toward genetically engineering the biosynthesis of taxoids in angiosperms. The first committed step in Taxol biosynthesis is the production of taxadiene from geranylgeranyl diphosphate (GGPP), catalyzed by the plastid‐localized enzyme taxadiene synthase (TXS). A recombinant T. baccata TXS lacking the putative plastid targeting peptide and fused to a C‐terminal histidine (His) tag was shown to be enzymatically active in Escherichia coli. Constitutive production of the full‐length His‐tagged enzyme in Arabidopsis thaliana plants led to the accumulation of taxadiene and concomitant growth retardation and decreased levels of photosynthetic pigment in transgenic plants. Although these phenotypes may derive from a toxic effect of taxadiene, the lower accumulation of endogenous plastid isoprenoid products such as carotenoids and chlorophylls in transgenic plants also suggests that the constitutive production of an active TXS enzyme might alter the balance of the GGPP pool. Induction of transgene expression using a glucocorticoid‐mediated system consistently resulted in a more efficient recruitment of GGPP for the production of taxadiene, which reached levels 30‐fold higher than those in plants constitutively expressing the transgene. This accomplishment illustrates the possibility of engineering the production of taxoids and other GGPP‐derived isoprenoids in crop plants despite the constraints associated with limited knowledge with regard to regulation of GGPP availability. © 2004 Wiley Periodicals, Inc.
Tools for Glycomics: Mapping Interactions of Carbohydrates in Biological Systems
Authors: Ratner Daniel M. , Adams Eddie W. , Disney Matthew D. , Seeberger Peter H. 
Published: September 2004

AbstractThe emerging field of glycomics has been challenged by difficulties associated with studying complex carbohydrates and glycoconjugates. Advances in the development of synthetic tools for glycobiology are poised to overcome some of these challenges and accelerate progress towards our understanding of the roles of carbohydrates in biology. Carbohydrate microarrays, fluorescent neoglycoconjugate probes, and aminoglycoside antibiotic microarrays are among the many new tools becoming available to glycobiologists.
Palladium‐Catalyzed Enantioselective Allylic Alkylation of Thiocarboxylated Ions: Asymmetric Synthesis of Allylic Thioesters and Memory Effect/Dynamic Kinetic Resolution of Allylic Esters
Authors: Luessem Bernhard J. , Gais Hans‐Joachim 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of ω‐Hydroxy Ketones from ω‐Benzyloxy Weinreb Amides by Using a Chemoselective Nucleophilic Addition/Birch Reduction Process.
Authors: Taillier Catherine , Bellosta Veronique , Meyer Christophe , Cossy Janine 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Dynamic Kinetic Resolution: An Efficient Route to anti α‐Amino‐β‐hydroxy Esters via Ru‐SYNPHOS® Catalyzed Hydrogenation.
Authors: Mordant Celine , Duenkelmann Pascal , Ratovelomanana‐Vidal Virginie , Genet Jean‐Pierre 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactions of Heterocumulenes with Organometallic Reagents. Part 9. Synthesis and Rearrangements of N‐Allyl‐ and N‐[2‐(Vinyloxy)ethyl]‐3‐butenethioamides and ‐1‐(Methylsulfanyl)‐3‐buten‐1‐imines.
Authors: Dmitrieva L. L. , Nikitina L. P. , Albanov A. I. , Sarapulova G. I. , Nedolya N. A. , Brandsma L. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Iron‐Catalyzed Propylene Epoxidation by Nitrous Oxide: Dramatic Shift of Allylic Oxidaton to Epoxidation by the Modification with Alkali Metal Salts.
Authors: Wang Xiaoxing , Zhang Qinghong , Guo Qian , Lou Yinchuan , Yang Lujuan , Wang Ye 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Development of a New Dimeric Cyclophane Ligand: Application to Enhanced Diastereo‐ and Enantioselectivity in the Catalytic Synthesis of β‐Lactams.
Authors: Wack Harald , France Stefan , Hafez Ahmed M. , Drury William J. III , Weatherwax Anthony , Lectka Thomas 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium‐Catalyzed Synthesis of N‐Aryl Pyrrolidines from γ‐(N‐Arylamino) Alkenes: Evidence for Chemoselective Alkene Insertion into Pd—N Bonds.
Authors: Ney Joshua E. , Wolfe John P. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium(0)‐Catalyzed Stereoselective Cyclization of Allenenes: Divergent Synthesis of Pyrrolidines and 3‐Azabicyclo[3.1.0]hexanes from Single Allenenes.
Authors: Ohno Hiroaki , Takeoka Yusuke , Kadoh Yoichi , Miyamura Kumiko , Tanaka Tetsuaki 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
N2‐Benzyl‐N1‐(1‐ (1‐naphthyl)ethyl)‐3‐phenylpropane‐1,2‐diamines and Conformationally Restrained Indole Analogues: Development of Calindol as a New Calcimimetic Acting at the Calcium Sensing Receptor.
Authors: Kessler Albane , Faure Helene , Petrel Christophe , Ruat Martial , Dauban Philippe , Dodd Robert H. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Comparative Study on the Synthesis of 3′‐Aryl‐3‐methyl/2, 3‐Dimethylspiro[6,7,8,9‐tetrahydro‐5H‐benzo[a]cyclohepten‐5, 2′‐(Tetrahydro[1,3]thiazolane)]‐4′‐ones Using Microwave Irradiation and Conventional Methods.
Authors: Venkateswarlu Peesapati , Vasireddy Nageswara Rao 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Mild and Efficient Synthesis of 4,5‐Disubstituted 3‐Amino‐4‐hydroxythiazolidine‐2‐thiones and S,N‐Disubstituted 1,3,4‐Thiadiazole‐2‐thiones.
Authors: Ge Ze‐mei , Cui Jia‐ling , Wang Yan‐ling , Cheng Tie‐ming , Li Run‐tao 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Design and Synthesis of Oxadiazolidinediones as Inhibitors of Plasminogen Activator Inhibitor‐1.
Authors: Gopalsamy Ariamala , Kincaid Scott L. , Ellingboe John W. , Groeling Thomas M. , Antrilli Thomas M. , Krishnamurthy Girija , Aulabaugh Ann , Friedrichs Gregory S. , Crandall David L. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Photoisomerization of 3‐Cyano‐6,6‐dimethyl‐4‐(4‐nitrophenylvinyl)‐5,6‐dihydropyran‐2‐one.
Authors: Jansone D. , Fleisher M. , Andreeva G. , Leite L. , Popelis J. , Lukevics E. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
DBU‐Induced Phenol‐Keto Resonance in 3,5‐Dihydroxyphthalide: Regioselectivities in Condensations with α,β‐Unsaturated Aldehydes — Facile Synthesis of Bioactive Natural and Unnatural Benzopyrans.
Authors: Mondal Mukulesh , Argade Narshinha P. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Electrochemical Oxidation of Nitriles of 4‐Aryl‐2‐carbamoylmethylthio‐5‐ethoxycarbonyl‐1,4‐dihydropyridine‐3‐carb oxylic Acids.
Authors: Baumane L. , Krauze A. , Chernova L. , Sile L. , Duburs G. , Stradins J. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Sonochemical Synthesis of 3‐ and 4‐Electron Withdrawing Ring Substituted N‐Alkyl‐1,8‐naphthalimides from the Related Anhydrides.
Authors: Triboni Eduardo Rezende , Berci Filho Pedro , Gomes de Souza Berlinck Roberto , Politi Mario Jose 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solvent‐Free Efficient Synthesis of Symmetrical Isocyanurates by a Combination Catalyst: Sodium Saccharin and Tetrabutylammonium Iodide.
Authors: Moghaddam Firouz Matloubi , Koozehgiri Gholam R. , Dekamin Mohammad G. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Unusual Recyclization of 2(1‐Bromostyryl)‐4‐oxo‐1,3‐benzoxazinium Perchlorate into 2,3,4,5‐Tetrahydro‐1,4‐benzoxazepine‐4,5‐dione.
Authors: Vikrishchuk N. P. , Suzdalev K. F. , Ryabukhin Yu. I. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Structure of 1‐ and 2‐Isomers of (Trimethoxysilylmethyl)‐ and (Silatranylmethyl)benzotriazole.
Authors: Voronkov M. G. , Trofimova O. M. , Bolgova Yu. I. , Klyba L. V. , Larina L. I. , Albanov A. I. , Pestunovich V. A. , Chernov N. F. , Petrushenko K. B. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Selenophene Synthesis in Thermal Reactions of Dialkyl Selenides with Acetylene in the Presence of Methanol.
Authors: Deryagina E. N. , Sukhomazova E. N. , Levanova E. P. , Korchevin N. A. , Danilova A. P. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nickel‐Catalyzed Tandem Carbostannylation of Alkynes and 1,2‐Dienes with Alkynylstannanes.
Authors: Shirakawa Eiji , Yamamoto Youko , Nakao Yoshiaki , Oda Shinichi , Tsuchimoto Teruhisa , Hiyama Tamejiro 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Studies Toward Taxuspine X (II), a Potent Multidrug‐Resistance Reversing Agent, via Ring Closing Metathesis Strategy
Authors: Renzulli Michela L. , Rocheblave Luc , Avramova Stanislava , Corelli Federico , Botta Maurizio 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Optimization of Allylic Oxidation of (1S,3R,8R)‐2,2‐Dichloro‐3,7,7,10‐tetramethyltricyclo [6,4,0,01,3]dodec‐9‐ene.
Authors: Dakir M. , Auhmani A. , Itto My. Y. Ait , Mazoir N. , Akssira M. , Pierrot M. , Benharref A. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel 17 Substituted Pregnadiene Derivatives as 5α‐Reductase Inhibitors and Their Binding Affinity for the Androgen Receptor.
Authors: Cabeza Marisa , Flores Eugenio , Heuze Ivonne , Sanchez Mauricio , Bratoeff Eugene , Ramirez Elena , Francolugo Victor Alfonso 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Reductive Alkylation of Alanylproline by the Ethyl Esters of 4‐Substituted 2‐Oxobutenoic Acids.
Authors: Slavinska V. , Sile Dz. , Chipens G. I. , Balodis Yu. , Rozenthal G. , Venteris K. , Lukevics E. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
An Advanced Intermediate for the Synthesis of (.+‐.)‐Pumiliotoxin C and Its Analogues.
Authors: Shao Zhi‐Hui , Peng Fang‐Zhi , Chen Jing‐Bo , Wang Chen‐Ying , Huang Rong , Tu Yong‐Qiang , Li Liang , Zhang Hong‐Bin 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Cytotoxic Activity of Pyranocarbazole Analogues of Ellipticine and Acronycine.
Authors: Tran‐Thi Hong Anh , Nguyen‐Thi Thuan , Michel Sylvie , Tillequin Francois , Koch Michel , Pfeiffer Bruno , Pierre Alain , Trinh‐Van‐Dufat Hanh 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
anti‐AIDS Agents. Part 58. Synthesis and anti‐HIV Activity of 1‐Thia‐di‐O‐(‐)‐camphanoyl‐(+)‐cis‐khellactone (1‐Thia‐DCK) Analogues.
Authors: Xia Peng , et al. et al. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Single‐Walled Carbon Nanotubes Filled with MOH (M: K, Cs) and Then Washed and Refilled with Clusters and Molecules.
Authors: Thamavaranukup Narun , Hoeppe Henning A. , Ruiz‐Gonzalez Luisa , Costa Pedro M. F. J. , Sloan Jeremy , Kirkland Angus , Green Malcolm L. H. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Sterically Hindered Phenols on the Basis of 3,5‐Di‐tert‐butyl‐4‐hydroxybenzyl Acetate
Authors: Bukharov S. V. , Nugumanova G. N. , Mukmeneva N. A. , Burilov A. R. , Kasymova E. M. , Pudovik M. A. , Konovalov A. I. 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Diaryl Diselenides and Benzisoselenazol‐3(2H)‐ones as Oxygen‐Transfer Agents
Authors: Mlochowski Jacek , Brzaszcz Monika , Chojnacka Magdalena , Giurg Miroslaw , Wojtowicz Halina 
Published: September 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Systematic review of adjuvant care for women with Stage I ovarian carcinoma
Authors: Elit Laurie , Chambers Alexandra , Fyles Anthony , Covens Allan , Carey Mark , Kee Fung Michael Fung 
Published: September 2004

AbstractBACKGROUNDSeveral adjuvant care interventions to treat women with Stage I ovarian carcinoma have been studied. The aim of the current systematic review was to determine the optimal strategy for adjuvant care for women with Stage I ovarian carcinoma.METHODSA systematic search was conducted to find randomized controlled trials published between 1965 and April 2004 that examined adjuvant therapy (e.g., chemotherapy and radiotherapy) for women with Stage I ovarian carcinoma.RESULTSThirteen randomized controlled trials were identified that compared adjuvant therapies for women with Stage I ovarian carcinoma. Eight of these trials reported results only for patients with Stage I disease. The majority of patients in the five randomized trials that compared adjuvant chemotherapy with no chemotherapy did not receive lymphadenectomy as part of their surgical staging. The pooled results for Stage I patients indicated a survival benefit (relative risk [RR], 0.74; 95% confidence interval [CI], 0.58–0.94; P = 0.01), and a benefit in terms of a reduced risk of developing disease recurrence (RR, 0.70; 95% CI, 0.58–0.86; P = 0.0004) favoring adjuvant chemotherapy. Platinum‐based adjuvant chemotherapy was reported to improve overall 5‐year survival (absolute survival difference 8%; 95% CI, 2–12%; hazard ratio, 0.67; 95% CI, 0.50–0.90; P = 0.008).CONCLUSIONSAdjuvant platinum‐based chemotherapy for women with Stage I ovarian carcinoma improved survival and reduced the risk of recurrent disease. The optimally staged group accounted for approximately 10% of women with Stage I disease. The role of adjuvant chemotherapy in optimally staged patients (especially those with good prognostic factors) has not been assessed adequately. Cancer 2004. © 2004 American Cancer Society.
Invasive aspergillosis in patients with solid tumors
Authors: Ohmagari Norio , Raad Isaam I. , Hachem Ray , Kontoyiannis Dimitrios P. 
Published: September 2004

AbstractInvasive aspergillosis (IA) has been reported only rarely among patients with solid tumors. In the current study, the authors retrospectively identified 13 episodes of definite or probable IA (using European Organization for Research and Treatment of Cancer criteria) occurring in patients with solid tumors who were treated between 1994–2003. Nine patients had pulmonary IA and three patients were found to have IA of the brain. Seven patients (54%) had primary or metastatic brain tumors and 6 patients (46%) received systemic steroids within 30 days prior to the diagnosis of IA. The majority of patients (69%) had a lymphocyte count < 500/μL but only 1 patient was neutropenic within the 30 days before the diagnosis of IA was made. Nodular infiltrates and cavities were the most common radiologic findings. Seven patients (54%) responded to antifungal treatment. Cancer 2004. © 2004 American Cancer Society.
Twitch and nontwitch motoneuron subgroups in the oculomotor nucleus of monkeys receive different afferent projections
Authors: Wasicky Richard , Horn Anja K.E. , Büttner‐Ennever Jean A. 
Published: September 2004

AbstractMotoneurons in the primate oculomotor nucleus can be divided into two categories, those supplying twitch muscle fibers and those supplying nontwitch muscle fibers. Recent studies have shown that twitch motoneurons lie within the classical oculomotor nucleus (nIII), and nontwitch motoneurons lie around the borders. Nontwitch motoneurons of medial and inferior rectus are in the C group dorsomedial to nIII, whereas those of inferior oblique and superior rectus lie near the midline are in the S group. In this anatomical study, afferents to the twitch and nontwitch subgroups of nIII have been anterogradely labeled by injections of tritiated leucine into three areas and compared. 1) Abducens nucleus injections gave rise to silver grain deposits over all medial rectus subgroups, both twitch and nontwitch. 2) Laterally placed vestibular complex injections that included the central superior vestibular nucleus labeled projections only in twitch motoneuron subgroups. However, injections into the parvocellular medial vestibular nucleus (mvp), or Y group, resulted in labeled terminals over both twitch and nontwitch motoneurons. 3) Pretectal injections that included the nucleus of the optic tract (NOT), and the olivary pretectal nucleus (OLN), labeled terminals only over nontwitch motoneurons, in the contralateral C group and in the S group. Our study demonstrates that twitch and nontwitch motoneuron subgroups do not receive identical afferent inputs. They can be controlled either in parallel, or independently, suggesting that they have basically different functions. We propose that twitch motoneurons primarily drive eye movements and nontwitch motoneurons the tonic muscle activity, as in gaze holding and vergence, possibly involving a proprioceptive feedback system. J. Comp. Neurol. 479:117–129, 2004. © 2004 Wiley‐Liss, Inc.
Low density of membrane particles in auditory hair cells of lizards and birds suggests an absence of somatic motility
Authors: Köppl Christine , Forge Andrew , Manley Geoffrey A. 
Published: September 2004

AbstractHair cells are the mechanoreceptive cells of the vertebrate lateral line and inner ear. In addition to their sensory function, hair cells display motility and thus themselves generate mechanical energy, which is thought to enhance sensitivity. Two principal cellular mechanism are known that can mediate hair‐cell motility in vitro. One of these is based on voltage‐dependent changes of an intramembrane protein and has so far been demonstrated only in outer hair cells of the mammalian cochlea. Correlated with this, the cell membranes of outer hair cells carry an extreme density of embedded particles, as revealed by freeze fracturing. The present study explored the possibility of membrane‐based motility in hair cells of nonmammals, by determining their density of intramembrane particles. Replicas of freeze‐fractured membrane were prepared from auditory hair cells of a lizard, the Tokay gecko, and a bird, the barn owl. These species were chosen because of independent evidence for active cochlear mechanics, in the form of spontaneous otoacoustic emissions. For quantitative comparison, mammalian inner and outer hair cells, as well as vestibular hair, cells were reevaluated. Lizard and bird hair cells displayed median densities of 2,360 and 1,880 intramembrane particles/μm2, respectively. This was not significantly different from the densities in vestibular and mammalian inner hair cells; however, it was about half the density in of mammalian outer hair cells. This suggests that nonmammalian hair cells do not possess high densities of motor protein in their membranes and are thus unlikely to be capable of somatic motility. J. Comp. Neurol. 479:149–155, 2004. © 2004 Wiley‐Liss, Inc.