bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatic Zbtb18 (Zinc Finger and BTB Domain Containing 18) alleviates hepatic steatohepatitis via FXR (Farnesoid X Receptor)
Authors: Zhang Lei , Chen Jiabing , Yang Xiaoying , Shen Chuangpeng , Huang Jiawen , Zhang Dong , Liu Naihua , Liu Chaonan , Zhong Yadi , Chen Yingjian , Tang Kaijia , Guo Jingyi , Cui Tianqi , Duan Siwei , Li Jiayu , Huang Shangyi , Pan Huafeng , Zhang Huabing , Tang Xiaoqiang , Chang Yongsheng , Gao Yong 
Published: January 2024

AbstractA lasting imbalance between fatty acid synthesis and consumption leads to non-alcoholic fatty liver disease (NAFLD), coupled with hepatitis and insulin resistance. Yet the details of the underlying mechanisms are not fully understood. Here, we unraveled that the expression of the transcription factor Zbtb18 is markedly decreased in the livers of both patients and murine models of NAFLD. Hepatic Zbtb18 knockout promoted NAFLD features like impaired energy expenditure and fatty acid oxidation (FAO), and induced insulin resistance. Conversely, hepatic Zbtb18 overexpression alleviated hepato-steatosis, insulin resistance, and hyperglycemia in mice fed on a high-fat diet (HFD) or in diabetic mice. Notably, in vitro and in vivo mechanistic studies revealed that Zbtb18 transcriptional activation of Farnesoid X receptor (FXR) mediated FAO and Clathrin Heavy Chain (CLTC) protein hinders NLRP3 inflammasome activity. This key mechanism by which hepatocyte’s Zbtb18 expression alleviates NAFLD and consequent liver fibrosis was further verified by FXR’s deletion and forced expression in mice and cultured mouse primary hepatocytes (MPHs). Moreover, CLTC deletion significantly abrogated the hepatic Zbtb18 overexpression-driven inhibition of NLRP3 inflammasome activity in macrophages. Altogether, Zbtb18 transcriptionally activates the FXR-mediated FAO and CLTC expression, which inhibits NLRP3 inflammasome’s activity alleviating inflammatory stress and insulin resistance, representing an attractive remedy for hepatic steatosis and fibrosis.
The safety and efficacy of systemic delivery of a new liver-de-targeted TGFβ signaling inhibiting adenovirus in an immunocompetent triple negative mouse mammary tumor model
Authors: Shin Soon Cheon , Vickman Renee E. , Filimon Beniamin , Yang Yuefeng , Hu Zebin , Mangold Kathy A. , Prabhakar Bellur S. , Schreiber Hans , Xu Weidong 
Published: January 2024

AbstractAberrant TGFβ signaling is linked to metastasis and tumor immune escape of many cancers including metastatic triple negative breast cancer (mTNBC). Previously, we have found that oncolytic adenoviruses expressing a TGFβ signaling inhibitory protein (sTGFβRIIFc) induced immune activation in a mouse TNBC (4T1) immunocompetent subcutaneous model with intratumoral injection. Systemic administration of adenoviruses can be a superior route to treat mTNBC but faces the challenges of increased toxicity and viral clearance. Thus, we created a liver-de-targeted sTGFβRIIFc- and LyP-1 peptide-expressing adenovirus (mHAdLyp.sT) with enhanced breast cancer cell tropism. Its safety and immune response features were profiled in the 4T1 model. Our data showed that the systemic administration of mHAdLyp.sT resulted in reduced hepatic and systemic toxicity. mHAdLyp.sT was also effective in increasing Th1 cytokines and anti-tumor cell populations by cytokine analysis, spleen/tumor qRT-PCR, and flow cytometry. We further tested the therapeutic effects of mHAdLyp.sT alone and in combination with immune checkpoint inhibitors (ICIs). mHAdLyp.sT alone and with all ICI combinations elicited significant inhibition of lung metastasis by histological analysis. When mHAdLyp.sT was combined with both anti-PD-1 and anti-CTLA-4 antibodies, primary 4T1 tumor growth was also significantly inhibited. We are confident in advancing this new treatment option for mTNBC.
Respiratory distress in SARS-CoV-2 exposed uninfected neonates followed in the COVID Outcomes in Mother-Infant Pairs (COMP) Study
Authors: Man Olivia M. , Azamor Tamiris , Cambou Mary Catherine , Fuller Trevon L. , Kerin Tara , Paiola Sophia G. , Cranston Jessica S. , Mok Thalia , Rao Rashmi , Chen Weiqiang , Jung Jae U. , Martinez Viviana Fajardo , Foo Suan-Sin , Nielsen-Saines Karin 
Published: January 2024

AbstractRespiratory distress (RD) has been reported in SARS-CoV-2 exposed uninfected (SEU) term neonates. Prior studies suggest that prenatal exposure to Coronavirus Disease 19 (COVID-19) may activate an inflammatory cascade in the newborn airway. In this study, we examine the relationship between maternal COVID-19 vaccination and neonatal RD using a longitudinal cohort of mother-infant pairs in Los Angeles, CA. Two-hundred and twenty-one mothers with laboratory confirmed SARS-CoV-2 during pregnancy and 227 exposed fetuses are enrolled in our study. Maternal disease severity and neonatal RD variables were defined based on current accepted clinical criteria. To explore the multifactorial associations between maternal COVID-19 parameters and infant RD, we utilize a multivariable logistic regression model and a proteomic sub-analysis to propose a pathway for the development of RD followingin uteroexposure to SARS-CoV-2. Unusually high rates of RD are observed in SEU infants (17%). The odds ratio of RD is 3.06 (95% CI:1.08-10.21) in term neonates born to unvaccinated individuals versus those born to individuals vaccinated prior to maternal infection. Proteomic analysis reveals a robust inflammatory response associated with ciliary dysregulation and enhanced IgE production among SEU neonates with RD. Maternal vaccination against COVID-19 reduces the frequency of neonatal RD.
Shape-changing electrode array for minimally invasive large-scale intracranial brain activity mapping
Authors: Wei Shiyuan , Jiang Anqi , Sun Hongji , Zhu Jingjun , Jia Shengyi , Liu Xiaojun , Xu Zheng , Zhang Jing , Shang Yuanyuan , Fu Xuefeng , Li Gen , Wang Puxin , Xia Zhiyuan , Jiang Tianzi , Cao Anyuan , Duan Xiaojie 
Published: January 2024

AbstractLarge-scale brain activity mapping is important for understanding the neural basis of behaviour. Electrocorticograms (ECoGs) have high spatiotemporal resolution, bandwidth, and signal quality. However, the invasiveness and surgical risks of electrode array implantation limit its application scope. We developed an ultrathin, flexible shape-changing electrode array (SCEA) for large-scale ECoG mapping with minimal invasiveness. SCEAs were inserted into cortical surfaces in compressed states through small openings in the skull or dura and fully expanded to cover large cortical areas. MRI and histological studies on rats proved the minimal invasiveness of the implantation process and the high chronic biocompatibility of the SCEAs. High-quality micro-ECoG activities mapped with SCEAs from male rodent brains during seizures and canine brains during the emergence period revealed the spatiotemporal organization of different brain states with resolution and bandwidth that cannot be achieved using existing noninvasive techniques. The biocompatibility and ability to map large-scale physiological and pathological cortical activities with high spatiotemporal resolution, bandwidth, and signal quality in a minimally invasive manner offer SCEAs as a superior tool for applications ranging from fundamental brain research to brain-machine interfaces.
Rational strain design with minimal phenotype perturbation
Authors: Narayanan Bharath , Weilandt Daniel , Masid Maria , Miskovic Ljubisa , Hatzimanikatis Vassily 
Published: January 2024

AbstractDevising genetic interventions for desired cellular phenotypes remains challenging regarding time and resources. Kinetic models can accelerate this task by simulating metabolic responses to genetic perturbations. However, exhaustive design evaluations with kinetic models are computationally impractical, especially when targeting multiple enzymes. Here, we introduce a framework for efficiently scouting the design space while respecting cellular physiological requirements. The framework employs mixed-integer linear programming and nonlinear simulations with large-scale nonlinear kinetic models to devise genetic interventions while accounting for the network effects of these perturbations. Importantly, it ensures the engineered strain’s robustness by maintaining its phenotype close to that of the reference strain. The framework, applied to improve the anthranilate production in E. coli, devises designs for experimental implementation, including eight previously experimentally validated targets. We expect this framework to play a crucial role in future design-build-test-learn cycles, significantly expediting the strain design compared to exhaustive design enumeration.
Upcycling poly(succinates) with amines to N-substituted succinimides over succinimide anion-based ionic liquids
Authors: Wu Fengtian , Wang Yuepeng , Zhao Yanfei , Zeng Shaojuan , Wang Zhenpeng , Tang Minhao , Zeng Wei , Wang Ying , Chang Xiaoqian , Xiang Junfeng , Xie Zongbo , Han Buxing , Liu Zhimin 
Published: January 2024

AbstractThe chemical transformation of waste polymers into value-added chemicals is of significance for circular economy and sustainable development. Herein, we report upcycling poly(succinates) (PSS) with amines into N-substituted succinimides over succinimide anion-based ionic liquids (ILs, e.g, 1,8-diazabicyclo[5.4.0]undec-7-ene succinimide, [HDBU][Suc]). Assisted with H2O, [HDBU][Suc]) showed the best performance, which could achieve complete transformation of a series of PSS into succinimide derivatives and corresponding diols under mild and metal-free conditions. Mechanism investigation indicates that the cation-anion confined hydrogen-bonding interactions among IL, H2O, ester group, and amino/amide groups, strengthens nucleophilicity of the N atoms in amino/amide groups, and improves electrophilicity of carbonyl C atom in ester group. The attack of the amino/amide N atom on carbonyl C of ester group results in cleavage of carbonyl C-O bond in polyester and formation of amide group. This strategy is also effective for aminolysis of poly(trimethylene glutarate) to glutarimides, and poly(1,4-butylene adipate) to caprolactone diimides.
Pt-doped Ru nanoparticles loaded on ‘black gold’ plasmonic nanoreactors as air stable reduction catalysts
Authors: Sharma Gunjan , Verma Rishi , Masuda Shinya , Badawy Khaled Mohamed , Singh Nirpendra , Tsukuda Tatsuya , Polshettiwar Vivek 
Published: January 2024

AbstractThis study introduces a plasmonic reduction catalyst, stable only in the presence of air, achieved by integrating Pt-doped Ru nanoparticles on black gold. This innovative black gold/RuPt catalyst showcases good efficiency in acetylene semi-hydrogenation, attaining over 90% selectivity with an ethene production rate of 320 mmol g−1 h−1. Its stability, evident in 100 h of operation with continuous air flow, is attributed to the synergy of co-existing metal oxide and metal phases. The catalyst’s stability is further enhanced by plasmon-mediated concurrent reduction and oxidation of the active sites. Finite-difference time-domain simulations reveal a five-fold electric field intensification near the RuPt nanoparticles, crucial for activating acetylene and hydrogen. Kinetic isotope effect analysis indicates the contribution from the plasmonic non-thermal effects along with the photothermal. Spectroscopic and in-situ Fourier transform infrared studies, combined with quantum chemical calculations, elucidate the molecular reaction mechanism, emphasizing the cooperative interaction between Ru and Pt in optimizing ethene production and selectivity.
Manipulating hyperbolic transient plasmons in a layered semiconductor
Authors: Fu Rao , Qu Yusong , Xue Mengfei , Liu Xinghui , Chen Shengyao , Zhao Yongqian , Chen Runkun , Li Boxuan , Weng Hongming , Liu Qian , Dai Qing , Chen Jianing 
Published: January 2024

AbstractAnisotropic materials with oppositely signed dielectric tensors support hyperbolic polaritons, displaying enhanced electromagnetic localization and directional energy flow. However, the most reported hyperbolic phonon polaritons are difficult to apply for active electro-optical modulations and optoelectronic devices. Here, we report a dynamic topological plasmonic dispersion transition in black phosphorus via photo-induced carrier injection, i.e., transforming the iso-frequency contour from a pristine ellipsoid to a non-equilibrium hyperboloid. Our work also demonstrates the peculiar transient plasmonic properties of the studied layered semiconductor, such as the ultrafast transition, low propagation losses, efficient optical emission from the black phosphorus’s edges, and the characterization of different transient plasmon modes. Our results may be relevant for the development of future optoelectronic applications.
Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes
Authors: Settele Simon , Schrage C. Alexander , Jung Sebastian , Michel Elena , Li Han , Flavel Benjamin S. , Hashmi A. Stephen K. , Kruss Sebastian , Zaumseil Jana 
Published: January 2024

AbstractInorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors.
Valid and efficient entanglement verification with finite copies of a quantum state
Authors: Cieśliński Paweł , Dziewior Jan , Knips Lukas , Kłobus Waldemar , Meinecke Jasmin , Paterek Tomasz , Weinfurter Harald , Laskowski Wiesław 
Published: January 2024

AbstractDetecting entanglement in multipartite quantum states is an inherently probabilistic process, typically with a few measured samples. The level of confidence in entanglement detection quantifies the scheme’s validity via the probability that the signal comes from a separable state, offering a meaningful figure of merit for big datasets. Yet, with limited samples, avoiding experimental data misinterpretations requires considering not only the probabilities concerning separable states but also the probability that the signal came from an entangled state, i.e. the detection scheme’s efficiency. We demonstrate this explicitly and apply a general method to optimize both the validity and the efficiency in small data sets providing examples using at most 20 state copies. The method is based on an analytical model of finite statistics effects on correlation functions which takes into account both a Frequentist as well as a Bayesian approach and is applicable to arbitrary entanglement witnesses.
Mediation of collisionless turbulent dissipation through cyclotron resonance
Authors: Bowen Trevor A. , Bale Stuart D. , Chandran Benjamin D. G. , Chasapis Alexandros , Chen Christopher H. K. , Dudok de Wit Thierry , Mallet Alfred , Meyrand Romain , Squire Jonathan 
Published: January 2024

AbstractThe dissipation of turbulence in astrophysical systems is fundamental to energy transfer and heating in environments ranging from the solar wind and corona to accretion disks and the intracluster medium. Although turbulent dissipation is relatively well understood in fluid dynamics, astrophysical plasmas often exhibit exotic behaviour, arising from the lack of interparticle collisions, which complicates turbulent dissipation and heating in these systems. Recent observations by NASA’s Parker Solar Probe mission in the inner heliosphere have shed new light on the role of ion cyclotron resonance as a potential candidate for turbulent dissipation and plasma heating. Here, using in situ observations of turbulence and wave populations, we show that ion cyclotron waves provide a major pathway for dissipation and plasma heating in the solar wind. Our results support recent theoretical predictions of turbulence in the inner heliosphere, known as the helicity barrier, that suggest a role of cyclotron resonance in ion-scale dissipation. Taken together, these results provide important constraints for turbulent dissipation and acceleration efficiency in astrophysical plasmas.
Redefining the treponemal history through pre-Columbian genomes from Brazil
Authors: Majander Kerttu , Pla-Díaz Marta , du Plessis Louis , Arora Natasha , Filippini Jose , Pezo-Lanfranco Luis , Eggers Sabine , González-Candelas Fernando , Schuenemann Verena J. 
Published: January 2024

AbstractThe origins of treponemal diseases have long remained unknown, especially considering the sudden onset of the first syphilis epidemic in the late 15th century in Europe and its hypothesized arrival from the Americas with Columbus’ expeditions1,2. Recently, ancient DNA evidence has revealed various treponemal infections circulating in early modern Europe and colonial-era Mexico3–6. However, there has been to our knowledge no genomic evidence of treponematosis recovered from either the Americas or the Old World that can be reliably dated to the time before the first trans-Atlantic contacts. Here, we present treponemal genomes from nearly 2,000-year-old human remains from Brazil. We reconstruct four ancient genomes of a prehistoric treponemal pathogen, most closely related to the bejel-causing agent Treponema pallidum endemicum. Contradicting the modern day geographical niche of bejel in the arid regions of the world, the results call into question the previous palaeopathological characterization of treponeme subspecies and showcase their adaptive potential. A high-coverage genome is used to improve molecular clock date estimations, placing the divergence of modern T. pallidum subspecies firmly in pre-Columbian times. Overall, our study demonstrates the opportunities within archaeogenetics to uncover key events in pathogen evolution and emergence, paving the way to new hypotheses on the origin and spread of treponematoses.
Oligodendrocyte–axon metabolic coupling is mediated by extracellular K+ and maintains axonal health
Authors: Looser Zoe J. , Faik Zainab , Ravotto Luca , Zanker Henri S. , Jung Ramona B. , Werner Hauke B. , Ruhwedel Torben , Möbius Wiebke , Bergles Dwight E. , Barros L. Felipe , Nave Klaus-Armin , Weber Bruno , Saab Aiman S. 
Published: January 2024

AbstractThe integrity of myelinated axons relies on homeostatic support from oligodendrocytes (OLs). To determine how OLs detect axonal spiking and how rapid axon–OL metabolic coupling is regulated in the white matter, we studied activity-dependent calcium (Ca2+) and metabolite fluxes in the mouse optic nerve. We show that fast axonal spiking triggers Ca2+ signaling and glycolysis in OLs. OLs detect axonal activity through increases in extracellular potassium (K+) concentrations and activation of Kir4.1 channels, thereby regulating metabolite supply to axons. Both pharmacological inhibition and OL-specific inactivation of Kir4.1 reduce the activity-induced axonal lactate surge. Mice lacking oligodendroglial Kir4.1 exhibit lower resting lactate levels and altered glucose metabolism in axons. These early deficits in axonal energy metabolism are associated with late-onset axonopathy. Our findings reveal that OLs detect fast axonal spiking through K+ signaling, making acute metabolic coupling possible and adjusting the axon–OL metabolic unit to promote axonal health.
Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament
Authors: Liu Tianyang , Cao Luyan , Mladenov Miroslav , Jegou Antoine , Way Michael , Moores Carolyn A. 
Published: January 2024

AbstractRegulation of the assembly and turnover of branched actin filament networks nucleated by the Arp2/3 complex is essential during many cellular processes, including cell migration and membrane trafficking. Cortactin is important for actin branch stabilization, but the mechanism by which this occurs is unclear. Given this, we determined the structure of vertebrate cortactin-stabilized Arp2/3 actin branches using cryogenic electron microscopy. We find that cortactin interacts with the new daughter filament nucleated by the Arp2/3 complex at the branch site, rather than the initial mother actin filament. Cortactin preferentially binds activated Arp3. It also stabilizes the F-actin-like interface of activated Arp3 with the first actin subunit of the new filament, and its central repeats extend along successive daughter-filament subunits. The preference of cortactin for activated Arp3 explains its retention at the actin branch and accounts for its synergy with other nucleation-promoting factors in regulating branched actin network dynamics.
The first host plant dataset of Curculionidae Scolytinae of the world: miscellaneous Tribes
Authors: Marchioro Matteo , Vallotto Davide , Ruzzier Enrico , Besana Laura , Rossini Michele , Ortis Giacomo , Faccoli Massimo , Martinez-Sañudo Isabel 
Published: January 2024

AbstractTribes Coriacephilini, Corthylini, Cryphalini, Ernoporini, Trypophloeini, Xyloctonini, and Xyloterini (Coleoptera: Curculionidae; Scolytinae) include spermophagous, phloeophagous, and xylomycetophagous species. Besides direct damage caused by burrowing into host plant tissues, some species are vectors of aggressive pathogens causing plant dieback and death, with consequent economic and ecological relevance. The international trade in plants and wood products is one of the main pathways for the introduction of non-native species worldwide. In this context, data availability on host plants and their economic uses is essential in pest risk assessment and for planning effective detection and monitoring strategies against invasive species. This paper provides a complete and updated list of host plants, with economic categorization, for 2139 scolytine species.
Influence of energy poverty on agricultural water efficiency using a panel data study in China
Authors: Shi Hongxu , Zhang Yuehua , Bian Mengyan , Zhang Jun 
Published: January 2024

AbstractThe research attention is increasingly directed towards the effective integration of the 17 United Nations Sustainable Development Goals (SDGs) within the limitations of the real world and amidst intersectoral conflicts. In light of the inextricable relationship between irrigation and energy, the objective of this study is to identify potential avenues for achieving the SDG6 and SDG7 goals of enhancing water use efficiency in agriculture and eradicating energy poverty, respectively. Utilizing data from 30 Chinese provinces from 2002 to 2017, this study explores the dynamic influence of energy poverty on agricultural water efficiency with a system generalized method of moments methodology. The findings suggest that energy poverty may greatly reduce agricultural water efficiency. The heterogeneity study shows that when agricultural water efficiency grows, the negative impacts of energy poverty continue to fade. Based on an assessment of various processes, results suggest that non-farm employment and cropping structure modification is a prominent conduit via which energy poverty negatively influences agricultural water efficiency.