bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gelation of soybean oil with polybutadiene
Authors: Yilmaz Mustafa , Kusefoğlu Seli̇m H. 
Published: April 2005

AbstractIn this study self‐supporting, resilient, load bearing polybutadiene ‐ soybean oil gels were obtained. The gels were made by dissolving polybutadiene (PBD) in soybean oil (SO) and selectively crosslinking PBD with a free radical source. PBD concentration, free radical source concentration, and the temperature and time of the crosslinking reaction were varied, and the effects of these changes on the mechanical properties of the gels were examined. Our experiments show that successful gelation is possible within PBD concentration limits of 7.5 to 12%, peroxide concentration between 25 to 100% (based on PBD), temperature between 110°C and 130°C and reaction times of 3 hours with tert.butyl‐peroxybenzoate as the free radical source. The crosslinking reaction was followed by IR and H‐NMR spectra, and the crosslinking density was followed by compression testing and swelling behavior. Higher radical source concentration and higher PBD concentration gave gels with better mechanical properties. The spectra and the viscosity increase of SO extracted from the gels indicate that there is dimer and trimer formation of SO during the reaction. The spectra of the PBD extracted from gels indicate that SO was added to PBD in a small but measurable amount. Integration of peak intensities in the NMR spectrum of methylene groups of PBD and methylene groups of triglyceride indicated one triglyceride molecule for approximately 45 repeating units in PBD. The modulus of the best gel sample (PBD 10%. peroxide 50%) was 1.96 × 10−2 MPa. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 2240–2246, 2005
Structural chemoproteomics and drug discovery
Authors: Shin Dongkyu , Heo Yong‐Seok , Joo Lee Kyung , Min Kim Cheol , Min Yoon Jung , Il Lee Jae , Hyun Young‐Lan , Ho Jeon Young , Gyu Lee Tae , Myung Cho Joong , Ro Seonggu 
Published: April 2005

AbstractOur laboratories have developed several technologies to accelerate drug discovery process on the basis of structural chemoproteomics. They include SPS™ technology for the efficient determination of protein structures, SCP™ technology for the rapid lead generation and SDF™ technology for the productive lead optimization. Using these technologies, we could determine many 3D structures of target proteins bound with biologically active chemicals including the structure of phosphodiesterase 5/Viagra complex and obtain highly potent compounds in animal models of obesity, diabetes, cancer and inflammation. In this paper, we will discuss concepts and applications of structural chemoproteomics for drug discovery. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2005
Interaction and structural study of kinin peptide bradykinin and ganglioside monosialylated 1 micelle
Authors: Chatterjee Chiradip , Mukhopadhyay Chaitali 
Published: April 2005

AbstractPartitioning of small proteins and peptides from the aqueous to membrane phase is often coupled with folding. In this work we examine the binding and folding of the kinin peptide, bradykinin (BK), in the presence of the ganglioside monosialylated 1 (GM1) micelle. Using two‐dimensional NMR techniques, we have shown that at low concentration, GM1 micelle is able to induce a turn conformation to BK. A pulsed‐field gradient diffusion NMR study indicated that the peptide partitions into the GM1 micelle with a ΔGpart of −3.14 ± 0.03 kcal/mol. A saturation transfer difference (STD) NMR study indicated that the binding is mostly through hydrophobic residues. © 2005 Wiley Periodicals, Inc. Biopolymers 78: 197–205, 2005
Stoichiometric limitations in assembly of active recombinant retrovirus
Authors: Lei Pedro , Andreadis Stelios T. 
Published: April 2005

AbstractAlthough recombinant retroviruses are widely used in gene therapy and as gene transfer vehicles for basic biological studies, their titers are very low as compared to other recombinant viral systems, e.g., adenovirus. We investigated the rate‐limiting steps in production of LacZ‐encoding ecotropic (CRE BAG 2) and amphotropic (Ψ‐CRIP) retrovirus. We found that ecotropic retrovirus producer cells produced a large number of inactive viral particles because they were severely limited by the amount of mRNA that was packaged into viral capsids. Introduction of the gene for green fluorescence protein (GFP) increased retroviral titers 40‐fold, without affecting the viral matrix protein, p30, or the activity of reverse transcriptase. Surprisingly, while transfer of GFP gene increased retrovirus production, β‐gal activity and X‐gal titer decreased significantly. Quantitative real‐time polymerase chain reaction (PCR) showed that although producer cells synthesized similar amounts of both mRNAs, retroviral supernatants contained significantly lower amount of LacZ mRNA, possibly due to competition between LacZ and GFP mRNAs for encapsidation into virions. In contrast to ecotropic producers, introduction of GFP gene copies into amphotropic producers resulted in a moderate twofold increase in retrovirus production. However, delivery of genes encoding for the viral proteins gp70 and p30 increased virus production by fivefold, suggesting that amphotropic producers may also be limited by synthesis of structural viral proteins. Our data show that in addition to the amount of viral genome or proteins, assembly of viral components into active viral particles may limit production of high titer retroviral preparations. © 2005 Wiley Periodicals, Inc.
Searching Sequence Space: Two Different Approaches to Dihydrofolate Reductase Catalysis
Authors: Howell Elizabeth E. 
Published: April 2005

AbstractThere are numerous examples of proteins that catalyze the same reaction while possessing different structures. This review focuses on two dihydrofolate reductases (DHFRs) that have disparate structures and discusses how the catalytic strategies of these two DHFRs are driven by their respective scaffolds. The two proteins are E. coli chromosomal DHFR (Ec DHFR) and a type II R‐plasmid‐encoded DHFR, typified by R67 DHFR. The former has been described as a very well evolved enzyme with an efficiency of 0.15, while the latter has been suggested to be a model for a “primitive” enzyme that has not yet been optimized by evolution. This comparison underlines what is important to catalysis in these two enzymes and concurrently highlights fundamental issues in enzyme catalysis.
Generation of Novel Landomycins M and O through Targeted Gene Disruption
Authors: Luzhetskyy Andriy , Zhu Lili , Gibson Miranda , Fedoryshyn Marta , Dürr Clemens , Hofmann Carsten , Hoffmeister Dirk , Ostash Bohdan , Mattingly Cynthia , Adams Val , Fedorenko Victor , Rohr Jürgen , Bechthold Andreas 
Published: April 2005

AbstractTwo genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C‐11‐hydroxylation that occurs during landomycin biosynthesis. Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.
Convenient Access Both to Highly Antimalaria‐Active 10‐Arylaminoartemisinins, and to 10‐Alkyl Ethers Including Artemether, Arteether, and Artelinate
Authors: Haynes Richard K. , Chan Ho‐Wai , Ho Wing‐Yan , Ko Cliff Ki‐Fung , Gerena Lucia , Kyle Dennis E. , Peters Wallace , Robinson Brian L. 
Published: April 2005

AbstractAn economical phase‐transfer method is used to prepare 10‐arylaminoartemisinins from DHA and arylamines, and artemether, arteether, and artelinate from the corresponding alcohols. In vivo sc screens against Plasmodium berghei and P. yoelii in mice reveal that the p‐fluorophenylamino derivative 5 g is some 13 and 70 times, respectively, more active than artesunate; this reflects the very high sc activity of 10‐alkylaminoartemisinins. However, through the po route, the compounds are less active than the alkylaminoartemisinins, but still approximately equipotent with artesunate.
Overestimation of aortic valve gradient measured by Doppler echocardiography in patients with aortic stenosis
Authors: Sakthi Chellammal , Yee Henry , Kotlewski Adam 
Published: April 2005

AbstractWe describe a series of patients with suspected aortic stenosis who were noted to have a significant aortic valve gradient by Doppler echocardiography but only a minimal gradient during cardiac catheterization. Although the pressure recovery phenomenon partially explained this discrepancy, a difference still persisted, particularly in peak gradients. The overestimation occurred at much lower cardiac catheterization gradients than previously reported. Since many of our patients were noted to be hypertensive, this discrepancy might be more apparent in this patient population. © 2005 Wiley‐Liss, Inc.
Congenital superior vena cava obstruction causing anasarca and respiratory failure in a newborn: Successful transcatheter therapy
Authors: Ro Pamela S. , Hill Sharon L. , Cheatham John P. 
Published: April 2005

AbstractSuperior vena cava (SVC) obstruction is a rare entity in the pediatric population. It usually presents in association with either previous cardiac surgery or external compression from a neoplasm. We present the case of an infant born with congenital SVC obstruction and significant bilateral chylothorax and anasarca necessitating mechanical ventilation. Successful placement of an intravascular stent led to resolution of the chylothoraces with rapid clinical improvement. © 2005 Wiley‐Liss, Inc.
Differential release of cardiac enzymes after percutaneous coronary intervention
Authors: Matthews Mark A. , Kunselman Susan J. , Gascho Joseph A. , Gilchrist Ian C. 
Published: April 2005

AbstractWe hypothesized that using calcium channel blockers (CCBs) that dilate microvasculature during percutaneous coronary intervention (PCI) would result in lower postprocedural creatine phosphokinase (CPK). PCI can be complicated by elevated CPK that has been associated with impaired microvascular perfusion. Nitroglycerin (NTG), the conventional PCI vasodilator, dilates epicardial arteries but does not affect the microvasculature. We hypothesized that using CCBs that dilate the microvasculature would result in lower postprocedural CPK values. Patients (n = 816) without evidence of acute myonecrosis undergoing PCI were divided into two groups based on whether they received intracoronary NTG or CCB during PCI. Postprocedural CPK values were compared using a repeated‐measures ANOVA and a random coefficient model. By repeated‐measures analysis, the NTG group had CPK values of 88%, 83%, and 89% of the CCB group's CPK values at < 8, 8–14, and > 14 hr after PCI (P = 0.0080, 0.0002, and 0.0244), respectively. In a random coefficient model, the NTG group had CPK values 84%, 84%, and 89% of the CCB group's mean CPK values at 6, 12, and 18 hr after PCI (P = 0.0003, 0.0006, and 0.0403), respectively. Peak CPK values occurred earlier with CCB, although the maximal CPK was similar in both groups. Intracoronary CCB use is associated with an accelerated release of CPK after PCI compared with NTG. This is consistent with more efficient relief of microvascular obstruction with CCB. It suggests that myonecrosis may originate with vascular trauma at the time of PCI and its enzymatic expression is modifiable with different vasodilators. © 2005 Wiley‐Liss, Inc.
Renal frame count: A quantitative angiographic assessment of renal perfusion
Authors: Mulumudi Mahesh S. , White Christopher J. 
Published: April 2005

AbstractRenal perfusion may be measured by a variety of noninvasive methods; however, there is no objective angiographic method to assess renal perfusion. We measured the renal frame count (RFC) in 26 patients (50 kidneys) with normal renal function and normal renal angiograms and 9 patients (15 kidneys) with renal artery fibromuscular dysplasia (FMD) and normal renal function. The mean age of the patients with normal renal arteries was 61.5 ± 9.5 (range, 47–82 years) and the mean age of patients with FMD was 72.5 ± 9.2 (range, 54–86 years; P = 0.005). There was no correlation between the age and RFC in both the normal renal artery group and the FMD group. The mean RFC for the normal renal arteries was 20.4 ± 3 (95% CI = 19.5–21.2), which was significantly lower than the FMD group's mean RFC of 26.9 ± 9.9 (95% CI = 21.4–32.4; P = 0.0001). RFC is an objective angiographic measure to quantify renal perfusion. Compared to normal renal arteries, those with FMD had significantly increased RFC consistent with decreased perfusion. © 2005 Wiley‐Liss, Inc.
Preparation of Superconducting Nax(H2O)yCoO2 Using NaMnO4 as the Deintercalation and Oxidation Agent.
Authors: Liu Chia‐Jyi , Hung Wen‐Chin , Wang Jung‐Sheng , Liu Chia‐Jung Charlie 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Assembly of Six‐Membered Vanadium Borophosphate Cluster Anions: Synthesis and Structures of (NH4)2 (C2H10N2)6 [Ba(H2O)5]2 [V2P2BO12] 6×8H2O and (NH4)8 (C3H12N2)4 [Ba(H2O)7] [V2P2BO12] 6×17H2O.
Authors: Yun Hoseop , Do Junghwan 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactivity of N1‐Tosylamidrazones with Thiophosphorus Trichloride and Carbon Disulfide. Access to New Triazaphospholines and Triazolethiones.
Authors: Mosbah Mongi Ben , Chouaib Hanen , Kossentini Mohamed , Salem Mansour 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Microwave Thermolysis. Part 9. A Rapid Reduction of Sulfoxides with NaH2PO2 in Dry Media.
Authors: Meshram H. M. , Ganesh Y. S. S. , Babu K. Ramesh , Eeshwaraiah B. , Yadav J. S. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Caro′s Acid Supported on Silica Gel. Part 6. A Mild Reagent for Regeneration of Carbonyl Compounds from Acetals, Ketals, and 1,1‐Diacetates.
Authors: Lakouraj M. M. , Tajbakhsh M. , Khojasteh V. , Gholami M. H. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 1,4‐Diethynyl‐ and 1,1,4,4‐Tetraethynylbutatrienes.
Authors: Auffrant Audrey , Diederich Francois , Boudon Corinne , Gisselbrecht Jean‐Paul , Gross Maurice 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Preparation of Fluoroalkyl Imines, Amines, Enamines, Ketones, α‐Amino Carbonyls, and α‐Amino Acids from Primary Enamine Phosphonates.
Authors: Palacios Francisco , Ochoa de Retana Ana Maria , Pascual Sergio , Oyarzabal Julen 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solvent‐Free Stereoselective Synthesis of cis‐1‐Carbomethoxy‐2‐aryl‐3,3‐dicyanocyclopropanes by Grinding.
Authors: Ren Zhongjiao , Cao Weiguo , Ding Weiyu , Shi Wen 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
The Multicomponent Reaction of Dimethoxycarbene, Dimethyl Butynedioate and Electrophilic Styrenes: An Unprecedented Synthesis of Highly Substituted Cyclopentenone Acetals.
Authors: Nair Vijay , Beneesh P. B. , Sreekumar V. , Bindu S. , Menon Rajeev S. , Deepthi Ani 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Convenient and Versatile Synthesis of Spiro(furan‐2,3‐indol[2]ones) and Spiro(pyran‐2,3‐indol[2]ones).
Authors: Smet Mario , Van Oosterwijck Chantal , Van Hecke Kristof , Van Meervelt Luc , Vandendriessche Annelies , Dehaen Wim 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 1,3‐Diaryl‐4‐cyanopyrazoles from the Corresponding Aldoximes Using Dimethylformamide‐thionylchloride Complex.
Authors: Reddy G. Jagath , Sailaja S. , Pallavi K. , Rao K. Srinivasa 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
2‐Acylimino‐3‐alkyl‐3H‐thiazoline Derivatives: One‐Pot, Three‐Component Condensation Synthesis of Novel β‐Turn Mimics.
Authors: Manaka Akira , Ishii Takaaki , Takahashi Keiko , Sato Masakazu 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient and Convenient Method for the Synthesis of Poly Functionalized 4H‐Pyrans.
Authors: Lingaiah B. P. V. , Reddy G. Venkat , Yakaiah T. , Narsaiah B. , Reddy S. N. , Yadla R. , Rao P. Shanthan 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
New 2‐Bromomethyl‐8‐substituted‐benzo[c]chromen‐6‐ones. Synthesis and Biological Properties.
Authors: Garino Cedrik , Bihel Frederic , Pietrancosta Nicolas , Laras Younes , Quelever Gilles , Woo Irene , Klein Peter , Bain Jenny , Boucher Jean‐Luc , Kraus Jean‐Louis 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis, Cardiovascular Activity, and Electrochemical Oxidation of Nitriles of 5‐Ethoxycarbonyl‐2‐methyl‐thio‐1,4‐dihydropyridine‐3‐carboxylic Acid.
Authors: Krauze A. , Baumane L. , Sile L. , Chernova L. , Vilums M. , Vitolina R. , Duburs G. , Stradins J. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Amino‐Substituted Heterocycles as Isosteres of trans‐Cinnamides: Design and Synthesis of Heterocyclic Biaryl Sulfides as Potent Antagonists of LFA‐1/ICAM‐1 Binding.
Authors: Wang Gary T. , Wang Sheldon , Gentles Robert , Sowin Thomas , Leitza Sandra , Reilly Edward B. , von Geldern Thomas W. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
4‐Anilinoquinazolines with Lavendustin A Subunit as Inhibitors of Epidermal Growth Factor Receptor Tyrosine Kinase: Syntheses, Chemical and Pharmacological Properties.
Authors: Albuschat Rica , Loewe Werner , Weber Manuela , Luger Peter , Jendrossek Verena 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Organoiodine (III) Mediated Synthesis of 3‐Aryl/Hetaryl‐5,7‐dimethyl‐1,2,4‐triazolo[4,3‐a]pyrimidines as Antibacterial Agents.
Authors: Prakash Om , Bhardwaj Vikas , Kumar Ravi , Tyagi Parikshit , Aneja Kamal R. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Direct Synthesis of Novel 2‐Imino‐1,3‐selenazolidine Derivatives from O‐Methanesulfonyl β‐Amino Alcohol Hydrochlorides.
Authors: Ueda Shigeo , Terauchi Hideo , Suzuki Kenji , Watanabe Nobuhide 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Peptide Coupling Method of Conjugated Carboxylic Acids with Methyl Ester Amino Acids Hydrochloride. Application to the Synthesis of Fa‐Met, an Important Enzymatic Substrate.
Authors: Brunel Jean Michel , Salmi Chanaz , Letourneux Yves 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Some Novel 6‐Benzyl(or Substituted Benzyl)‐2‐β‐D‐glucopyranosyl‐1,2,4‐triazolo[4,3‐b][1,2,4]triazines as Potential Antimicrobial Chemotherapeutics.
Authors: Khalil Nasser S. A. M. , Mansour Abdel Kader , Eid Mohga M. 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel 1β‐Methylcarbapenems with Isoxazoloethenyl Moieties Containing Carboxylic Acid Sodium Salt.
Authors: Lee Kyung Seok , Kang Yong Koo , Yoo Kyung Ho , Kim Dong Chan , Shin Kye Jung , Paik Young‐Sook , Kim Dong Jin 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Secondary Allyltitanium(IV) Reagents in Aldehyde Allylation. Part 1. Extension of the Hoppe Reaction to γ‐Alkoxy Secondary Allyl Carbamates.
Authors: Razon Patrick , Dhulut Sylvie , Bezzenine‐Lafollee Sophie , Courtieu Jacques , Pancrazi Ange , Ardisson Janick 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Himeic Acid A (I): A New Ubiquitin‐Activating Enzyme Inhibitor Isolated from a Marine‐Derived Fungus, Aspergillus sp.
Authors: Tsukamoto Sachiko , Hirota Hiroshi , Imachi Misako , Fujimuro Masahiro , Onuki Hiroyuki , Ohta Tomihisa , Yokosawa Hideyoshi 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Antitumor Agents. Part 236. Synthesis of Water‐Soluble Colchicine Derivatives
Authors: Nakagawa‐Goto Kyoko , Chen Cyril X. , Hamel Ernest , Wu Chin‐Chung , Bastow Kenneth F. , Brossi Arnold , Lee Kuo‐Hsiung 
Published: April 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
The prognostic significance of the percentage of positive/dissected axillary lymph nodes in breast cancer recurrence and survival in patients with one to three positive axillary lymph nodes
Authors: Truong Pauline T. , Berthelet Eric , Lee Junella , Kader Hosam A. , Olivotto Ivo A. 
Published: April 2005

AbstractBACKGROUNDAdjuvant therapy for women with T1–T2 breast carcinoma and 1–3 positive lymph nodes is controversial due to discrepancies in reported baseline locoregional recurrence (LRR) risks. This inconsistency has been attributed to variations in lymph node staging techniques, which have yielded different numbers of dissected lymph nodes. The current study evaluated the prognostic impact of the percentage of positive/dissected lymph nodes on recurrence and survival in women with one to three positive lymph nodes.METHODSThe study cohort was comprised of 542 women with pathologic T1–T2 breast carcinoma who had 1–3 positive lymph nodes and who had undergone mastectomy and received adjuvant systemic therapy without radiotherapy. Ten‐year Kaplan–Meier (KM) LRR, distant recurrence (DR), and overall survival (OS) rates stratified by the number of positive lymph nodes, the number of dissected lymph nodes, and the percentage of positive lymph nodes were examined using different cut‐off levels. Multivariate analysis was performed to evaluate the prognostic significance of the percentage of positive lymph nodes in disease recurrence and survival.RESULTSThe median follow‐up was 7.5 years. LRR, DR, and OS rates correlated significantly with the number of positive lymph nodes and the percentage of positive lymph nodes, but not with the number of dissected lymph nodes. The cut‐off level at which the most significant difference in LRR was observed was 25% positive lymph nodes (the 10‐year KM LRR rates were 13.9% and 36.7% in women with ≤ 25% and > 25% positive lymph nodes, respectively; P < 0.0001). Higher DR rates and lower OS rates were observed among patients who had > 25% positive lymph nodes compared with patients who had ≤ 25% positive lymph nodes (DR: 53.0% vs. 30.3%, respectively; P < 0.0001; OS: 43.4% vs. 62.6%, respectively; P < 0.0001). In the multivariate analysis, the percentage of positive lymph nodes and the histologic grade were significant, independent factors associated with LRR, DR, and OS.CONCLUSIONSThe presence of > 25% positive lymph nodes was an adverse prognostic factor in patients with 1–3 positive nodes and may be used to identify patients at high risks of postmastectomy locoregional and distant recurrence who may benefit with adjuvant radiotherapy and more aggressive systemic therapy regimens. Cancer 2005. © 2005 American Cancer Society.