bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prolonged tamoxifen‐enriched diet is associated with cardiomyopathy and nutritional frailty in mice
Authors: Halpage Janith , DaSilva Pantoja Patricia , Mancarella Salvatore 
Published: January 2024

AbstractTamoxifen (TAM) is required for gene recombination in the inducible Cre/lox system. The TAM‐enriched diet is considered safe, with negligible impact on animal wellbeing. However, studies reporting the long‐term effects of the TAM diet and its potential impact on experimental outcomes are scarce. We conducted a longitudinal study on mice exposed to a 4‐week dietary TAM citrate supplementation. Several parameters were recorded, such as body weight, body composition, mortality, and cardiac function. The collagen1a2 (Col1a2) transgenic mouse was used to assess TAM‐induced recombination in vivo in cardiac fibroblasts followed by myocardial infarction (MI). The impact of TAM on the MI outcome was also evaluated. The recombination efficiency and cytotoxic effect of the TAM active metabolite, 4‐hydroxy‐tamoxifen (4‐OHT), were assessed in vitro. Mice exposed to a TAM diet showed body weight loss and a 10% increase in mortality (P = 0.045). The TAM diet decreased cardiac function and induced cardiac remodeling, indicated by decreased fractional shortening from 32.23% to 19.23% (P = 0.001) and left ventricular (LV) wall thinning. All measured parameters were reversed to normal when mice were returned to a normal diet. Infarcted Col1a2‐CreER mice on the TAM regimen showed gene recombination in fibroblasts, but it was associated with a substantial increase in mortality post‐surgery (2.5‐fold) compared to the controls. In vitro, 4‐OHT induced gene editing in fibroblasts; however, cell growth arrest and cytotoxicity were observed at high concentrations. In conclusion, prolonged exposure to the TAM diet can be detrimental and necessitates careful model selection and interpretation of the results.
Research on a Biomimetic Flexible Ball Joint With Variable Stiffness for Robots
Authors: Qianyu Luo , Xiuli Zhang , Yuxin Wang 
Published: January 2024

Abstract
The human ball joint is a compact and flexible 3-degree-of-freedom (DOF) composite joint. The ball joints commonly used in robot design lack active driving capability. Ball joints with active drive generally consist of three single-degree-of-freedom joints connected in series, which is a noncompacted structure and easily leads to singular postures. In order to meet the demand for high-performance composite joint modules for service robots, this article designs a flexible biomimetic spherical robot joint with variable stiffness characteristics: the mechanism of muscle parallel antagonistic drive and ligament wrapping constraint is simulated; three parallel branch chains are used to drive three composite degrees-of-freedom; ropes, soft airbags, and series elastic drive gears are used to form a flexible transmission system; the contour of the rope winch has been optimized with the aim of transmission stability; and a pneumatic variable stiffness soft structure has been designed and fabricated. A compliance control algorithm for joints was developed based on the principle of impedance control. The research results indicate that the biomimetic ball-and-socket joint has a compact structure, a wide range of motion and good motion tracking performance, variable stiffness performance, and flexible interaction ability.
An Efficient Kinematic Calibration Method for Parallel Robots With Compact Multi-Degrees-of-Freedom Joint Models
Authors: Zhang Weijia , Shi Zikang , Chai Xinxue , Ding Ye 
Published: January 2024

Abstract
Forward kinematics-based modeling approaches are capable of constructing complete kinematic error models for parallel robots in a general way. The existing forward kinematics-based modeling methods replace multi-degrees-of-freedom (multi-DOF) joints with several 1DOF joints, allowing each limb of the parallel robot to be modeled like a serial robot. Nonetheless, this substitution complicates the kinematic model and results in additional computation. To overcome this limitation, an efficient kinematic calibration method adopting compact multi-DOF joint models is proposed. First, compact kinematic models for multi-DOF joints are established with the product of exponentials formula and adopted in the forward kinematic formulation of limbs. Error models of limbs are derived by simplifying the forward kinematic formulas' differentials, and the geometric error model for parallel robots is established by further concatenating and reformulating the limb error models. Next, the kinematic model is iteratively updated with the geometric parameter errors identified by the Levenberg–Marquardt algorithm. Error compensation is achieved through the inverse kinematics of the calibrated kinematic model. Finally, simulations and an experiment are implemented for validation. Compared with the existing forward kinematics-based modeling approaches, the error modeling procedures are simplified as the equivalent substitution of multi-DOF joints is avoided. The proposed approach also enhances the error compensation efficiency while maintaining high accuracy improvement.
Illegal dumping of oil and gas wastewater alters arid soil microbial communities
Authors: Kashani Mitra , Engle Mark A. , Kent Douglas B. , Gregston Terry , Cozzarelli Isabelle M. , Mumford Adam C. , Varonka Matthew S. , Harris Cassandra R. , Akob Denise M. 
Published: January 2024

ABSTRACTThe Permian Basin, underlying southeast New Mexico and west Texas, is one of the most productive oil and gas (OG) provinces in the United States. Oil and gas production yields large volumes of wastewater with complex chemistries, and the environmental health risks posed by these OG wastewaters on sensitive desert ecosystems are poorly understood. Starting in November 2017, 39 illegal dumps, as defined by federal and state regulations, of OG wastewater were identified in southeastern New Mexico, releasing ~600,000 L of fluid onto dryland soils. To evaluate the impacts of these releases, we analyzed changes in soil geochemistry and microbial community composition by comparing soils from within OG wastewater dump-affected samples to unaffected zones. We observed significant changes in soil geochemistry for all dump-affected compared with control samples, reflecting the residual salts and hydrocarbons from the OG-wastewater release (e.g., enriched in sodium, chloride, and bromide). Microbial community structure significantly (P< 0.01) differed between dump and control zones, with soils from dump areas having significantly (P< 0.01) lower alpha diversity and differences in phylogenetic composition. Dump-affected soil samples showed an increase in halophilic and halotolerant taxa, including members of the Marinobacteraceae, Halomonadaceae, and Halobacteroidaceae, suggesting that the high salinity of the dumped OG wastewater was exerting a strong selective pressure on microbial community structure. Taxa with high similarity to known hydrocarbon-degrading organisms were also detected in the dump-affected soil samples. Overall, this study demonstrates the potential for OG wastewater exposure to change the geochemistry and microbial community dynamics of arid soils.IMPORTANCEThe long-term environmental health impacts resulting from releases of oil and gas (OG) wastewater, typically brines with varying compositions of ions, hydrocarbons, and other constituents, are understudied. This is especially true for sensitive desert ecosystems, where soil microbes are key primary producers and drivers of nutrient cycling. We found that releases of OG wastewater can lead to shifts in microbial community composition and function toward salt- and hydrocarbon-tolerant taxa that are not typically found in desert soils, thus altering the impacted dryland soil ecosystem. Loss of key microbial taxa, such as those that catalyze organic carbon cycling, increase arid soil fertility, promote plant health, and affect soil moisture retention, could result in cascading effects across the sensitive desert ecosystem. By characterizing environmental changes due to releases of OG wastewater to soils overlying the Permian Basin, we gain further insights into how OG wastewater may alter dryland soil microbial functions and ecosystems.
Complete genome sequences of bacteriophages Guetzie and SirVictor, isolated from
Microbacterium foliorum
Authors: Ngove Zefanias , Matthews Raegen , Goedken Jack , Huntington Sophia , Kirkle Lauren , Coleman Sean T. 
Published: January 2024

ABSTRACT

Cluster EA4 Guetzie and SirVictor are lytic siphoviral bacteriophages that were isolated from soil in Waverly, Iowa, using
Microbacterium foliorum
NRRL B-24224 as the host. The Guetzie and SirVictor genomes are both 39,758 bp each, and both contain 58 predicted protein-coding genes with one tRNA gene each.
Scatterhoarders use seedlings as cues of belowground food availability
Authors: Ramirez Sarah B. , Steury Todd D. 
Published: January 2024

Animals benefit from scatterhoarding, storing food around their home range for use during a period of scarcity, by later eating the stored food. Seedlings may be used as cues of belowground food. We investigated whether scatterhoarders such as eastern gray squirrels ( Sciurus carolinensis Gmelin, 1788) and eastern chipmunks ( Tamias striatus (Linnaeus, 1758)) use characteristics of seedlings and their seeds as physical cues of the value of the seed. We buried 176 germinating seeds (white oak ( Quercus alba L.) and northern red oak ( Quercus rubra L.)) in a hardwood forest in Alabama, USA. Seeds were recovered faster with longer leaves (rate ratio (RR) = 0.68, 0.58–0.78, 95% confidence limits (C.L.)) and taller stems (RR = 0.91, 0.88–0.93, 95% C.L.) during spring 2022, and with a smaller number (RR = 1.15, 1.04–1.27, 95% C.L.) of longer leaves (RR = 0.81, 0.70–0.93, 95% C.L.) during fall 2022 (both P < 0.0001). For all seeds, we found that longer roots increased the likelihood of seeds being used (rather than ignored; odds ratio = 1.45, 1.00–2.09, 95% C.L., P = 0.05). We found new evidence suggesting that hoarders use aboveground cues from the seedling to dig it up and belowground seedling cues to assess seed value. These findings add to our understanding of assessing food availability for hoarding animals.
The thorium isomer $$^{229m}$$Th: review of status and perspectives after more than 50 years of research
Authors: Thirolf Peter G. , Kraemer Sandro , Moritz Daniel , Scharl Kevin 
Published: January 2024

AbstractToday’s most precise timekeeping is based on optical atomic clocks. However, those could potentially be outperformed by a nuclear clock, based on a nuclear transition instead of an atomic shell transition. Such a nuclear clock promises intriguing applications in applied as well as fundamental physics, ranging from geodesy and seismology to the investigation of possible time variations of fundamental constants and the search for dark matter. Only one nuclear state is known so far that could drive a nuclear clock: the “Thorium Isomer” $$^{229m}$$

229
m

Th, i.e., the isomeric first excited state of $$^{229}$$

229

Th, representing the lowest nuclear excitation so far reported in the landscape of nuclear isotopes. Indirectly conjectured to exist already in 1976, decades of experimental efforts were dedicated to unambiguously identify this elusive nuclear state and to characterize its properties. However, for 40 years, these efforts remained inconclusive. The turning point was marked by the first direct detection of $$^{229m}$$

229
m

Th via its internal conversion decay branch in 2016. Since then, remarkable progress could be achieved in characterizing the properties and decay parameters. The half-life of the neutral isomer was determined, the hyperfine structure was measured via collinear laser spectroscopy, providing information on nuclear moments and the nuclear charge radius and also the excitation energy of the isomer could be directly determined with different techniques. In a recent experiment at CERN’s ISOLDE facility, the long-sought radiative decay of the Thorium isomer could be observed for the first time via implantation of ($$\beta$$
β
-decaying) $$^{229}$$

229

Ac into a vacuum-ultraviolet (VUV) transparent crystal and subsequent fluorescence detection in a VUV spectrometer. Thus, the excitation energy of $$^{229m}$$

229
m

Th could be determined with unprecedented precision to 8.338(24) eV, corresponding to a wavelength of 148.71(42) nm. These achievements, together with ongoing laser developments for the required VUV wavelength, open the door toward a laser-driven control of the isomeric transition and thus to the development of an ultra-precise nuclear frequency standard.
Phase II Study of the Liposomal Formulation of Eribulin (E7389-LF) in Combination with Nivolumab: Results from the Gastric Cancer Cohort
Authors: Kawazoe Akihito , Yamamoto Noboru , Sugimoto Naotoshi , Kawakami Hisato , Oshima Takashi , Yamaguchi Kensei , Hino Kaori , Hirao Motohiro , Kurokawa Yukinori , Kawakami Takeshi , Tsuda Masahiro , Hara Hiroki , Kaname Shota , Matsuoka Daiko , Otake Yohei , Yasuda Keisuke , Takase Takao , Takashima Shuya , Semba Taro , Muro Kei 
Published: January 2024

Abstract

Purpose:
E7389-LF is a liposomal formulation of the microtubule dynamics inhibitor eribulin and has shown preliminary efficacy in the treatment of gastric cancer. Study 120, a phase Ib/II open-label study, assessed efficacy and safety of E7389-LF in combination with nivolumab, a programmed cell death (PD)-1 inhibitor. This report focuses on the gastric cancer cohort within the expansion phase.

Patients and Methods:
Eligible patients had unresectable, measurable gastric cancer, progression following a platinum drug plus fluoropyrimidine (1L), and a taxane-containing regimen (2L). The primary objective of the expansion phase was objective response rate, secondary objectives included safety and PFS, and exploratory objectives included overall survival and biomarker evaluation. Patients received E7389-LF 2.1 mg/m2 in combination with nivolumab 360 mg every 3 weeks, both as intravenous infusions. Tumor responses were assessed every 6 weeks by the investigators per RECIST v1.1. Plasma and tumor biomarkers were assessed.

Results:
In the 31 patients who received E7389-LF in combination with nivolumab, the objective response rate was 25.8% [confidence interval (CI), 11.9–44.6]. The median progression-free survival was 2.69 months (95% CI, 1.91–2.99) and median overall survival was 7.85 months (95% CI, 4.47–not estimable). The most common treatment-related TEAE of any grade were neutropenia (77.4%), leukopenia (74.2%), and decreased appetite (51.6%). E7389-LF in combination with nivolumab significantly increased CD8-positive cells at C2D1 (P = 0.039), and six of seven vascular markers and four IFNγ-related markers showed increases from C1D1.

Conclusions:
Promising antitumor activity was observed with E7389-LF in combination with nivolumab in patients with gastric cancer, and no new safety signals were observed, compared with either monotherapy.
Cerebrospinal Fluid cfDNA Sequencing for Classification of Central Nervous System Glioma
Authors: Iser Florian , Hinz Felix , Hoffmann Dirk C. , Grassl Niklas , Güngör Cansu , Meyer Jochen , Dörner Laura , Hofmann Lea , Kelbch Vanessa , Göbel Kirsten , Mahmutoglu Mustafa Ahmed , Vollmuth Philipp , Patel Areeba , Nguyen Duy , Kaulen Leon D. , Mildenberger Iris , Sahm Katharina , Maaß Kendra , Pajtler Kristian W. , Shankar Ganesh M. , Weiler Markus , Wildemann Brigitte , Winkler Frank , von Deimling Andreas , Platten Michael , Wick Wolfgang , Sahm Felix , Kessler Tobias 
Published: January 2024

Abstract

Purpose:
Primary central nervous system (CNS) gliomas can be classified by characteristic genetic alterations. In addition to solid tissue obtained via surgery or biopsy, cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is an alternative source of material for genomic analyses.

Experimental Design:
We performed targeted next-generation sequencing of CSF cfDNA in a representative cohort of 85 patients presenting at two neurooncological centers with suspicion of primary or recurrent glioma. Copy-number variation (CNV) profiles, single-nucleotide variants (SNV), and small insertions/deletions (indel) were combined into a molecular-guided tumor classification. Comparison with the solid tumor was performed for 38 cases with matching solid tissue available.

Results:
Cases were stratified into four groups: glioblastoma (n = 32), other glioma (n = 19), nonmalignant (n = 17), and nondiagnostic (n = 17). We introduced a molecular-guided tumor classification, which enabled identification of tumor entities and/or cancer-specific alterations in 75.0% (n = 24) of glioblastoma and 52.6% (n = 10) of other glioma cases. The overlap between CSF and matching solid tissue was highest for CNVs (26%–48%) and SNVs at predefined gene loci (44%), followed by SNVs/indels identified via uninformed variant calling (8%–14%). A molecular-guided tumor classification was possible for 23.5% (n = 4) of nondiagnostic cases.

Conclusions:
We developed a targeted sequencing workflow for CSF cfDNA as well as a strategy for interpretation and reporting of sequencing results based on a molecular-guided tumor classification in glioma.
From Expression to Connection: Decoding Sana All’s Linguistic Meanings in Communication Context
Authors: Dangalao Tong Kabenbelan 
Published: January 2024

very age group and generation uses different lingo, jargon, and forms of communication in casual conversations. These lingos could be introduced by the fashions, significant events, radio shows, movies, TV shows, songs, and literature of their time, among many other devices. Additionally, technology has changed some words, adding new ones that are more inventive and simpler to type.
The study aimed to analyze the linguistic meaning of "Sana All" used by the Grade 11 Senior High School students in Cotabato City National High School – Annex (Buaya-Buaya Site). Colaizzi’s phenomenological method of inquiry was employed in this study. The researcher utilized the Focal Group Discussion (FGD) in gathering data.
The study's findings revealed that the phrase "Sana All" is used by student participants in Social Media, School, Home, and the Real World. The phrase connotes admiration, appreciation, wishing for something to happen, and strengthening relationships with others. Various meanings were generated depending on the communication setting.
The researcher concluded that students use the phrase "Sana All" in various communication settings. They use this phrase when wishing, admiring, appreciating, and strengthening relationships with others.
Finally, the researcher produced a linguistic concept on the teaching strategies on pragmatics and semantics where the teacher can adopt the trendy phrase to be included when teaching the linguistic concept of pragmatics and semantics.
MicroRNA‐34a‐Dependent Attenuation of Angiogenesis in Right Ventricular Failure
Authors: Reddy Sushma , Hu Dong‐Qing , Zhao Mingming , Ichimura Shoko , Barnes Elizabeth A. , Cornfield David N. , Alejandre Alcázar Miguel A. , Spiekerkoetter Edda , Fajardo Giovanni , Bernstein Daniel 
Published: January 2024

Background
The right ventricle (RV) is at risk in patients with complex congenital heart disease involving right‐sided obstructive lesions. We have shown that capillary rarefaction occurs early in the pressure‐loaded RV. Here we test the hypothesis that microRNA (miR)‐34a, which is induced in RV hypertrophy and RV failure (RVF), blocks the hypoxia‐inducible factor‐1α‐vascular endothelial growth factor (VEGF) axis, leading to the attenuated angiogenic response and increased susceptibility to RV failure.

Methods and Results

Mice underwent pulmonary artery banding to induce RV hypertrophy and RVF. Capillary rarefaction occurred immediately. Although hypoxia‐inducible factor‐1α expression increased (0.12±0.01 versus 0.22±0.03,
P
=0.05), VEGF expression decreased (0.61±0.03 versus 0.22±0.05,
P
=0.01). miR‐34a expression was most upregulated in fibroblasts (4‐fold), but also in cardiomyocytes and endothelial cells (2‐fold). Overexpression of miR‐34a in endothelial cells increased cell senescence (10±3% versus 22±2%,
P
<0.05) by suppressing sirtulin 1 expression, and decreased tube formation by 50% via suppression of hypoxia‐inducible factor‐1α, VEGF A, VEGF B, and VEGF receptor 2. miR‐34a was induced by stretch, transforming growth factor‐β1, adrenergic stimulation, and hypoxia in cardiac fibroblasts and cardiomyocytes. In mice with RVF, locked nucleic acid‐antimiR‐34a improved RV shortening fraction and survival half‐time and restored capillarity and VEGF expression. In children with congenital heart disease–related RVF, RV capillarity was decreased and miR‐34a increased 5‐fold.

Conclusions
In summary, miR‐34a from fibroblasts, cardiomyocytes, and endothelial cells mediates capillary rarefaction by suppressing the hypoxia‐inducible factor‐1α‐VEGF axis in RV hypertrophy/RVF, raising the potential for anti‐miR‐34a therapeutics in patients with at‐risk RVs.
Clinical Characteristics and Rhythm Outcomes in Patients With Atrial Myopathy After Successful Catheter Ablation of Atrial Fibrillation
Authors: Kim Moon‐Hyun , Kim Tae‐Hoon , Hwang Inseok , Park Je‐Wook , Yu Hee Tae , Uhm Jae‐Sun , Joung Boyoung , Lee Moon‐Hyoung , Hwang Chun , Pak Hui‐Nam 
Published: January 2024

Background
Although successful atrial fibrillation (AF) ablation can maintain sinus rhythm and reduce the left atrial (LA) dimension, blunted LA reverse remodeling can be observed in patients with atrial myopathy. We explored the potential mechanisms and long‐term outcomes in patients with blunted LA reverse remodeling after successful AF catheter ablation.

Methods and Results

We included 1685 patients who underwent baseline and 1‐year follow‐up echocardiograms, had a baseline LA dimension ≥40 mm, and did not have a recurrence of AF within a year. The patients were divided into tertile groups according to the delta value of the change in LA dimension on the preprocedure and 1‐year postprocedure echocardiography. After propensity score matching for age, sex, AF type, and LA dimension, 1272 patients were finally included in the analyses (424 in each group; the least/blunted, moderate, and the most reverse remodeling group). The patients in the T1 group (blunted LA reverse remodeling) were independently associated with higher left ventricular mass index (odds ratio [OR], 1.014 [95% CI, 1.005–1.022],
P
=0.001), change in ΔH
2
FPEF score (heavy, hypertensive, atrial fibrillation, pulmonary hypertension, elder, filling pressure) score (OR, 1.445 [95% CI, 1.121–1.861],
P
=0.004), ventricular epicardial adipose tissue volume (OR, 1.010 [95% CI, 1.003–1.017],
P
=0.003), thinner LA wall thickness (OR, 0.461 [95% CI, 0.271–0.785],
P
=0.004), lower LA voltage (OR, 0.670 [95% CI, 0.499–0.899],
P
=0.008), and showed higher long‐term AF recurrence (log‐rank
P
<0.001) than other groups.

Conclusions

Blunted LA reverse remodeling after AF catheter ablation, which is suggestive of atrial myopathy, was independently associated with a larger ventricular epicardial adipose tissue volume and worsening of H
2
FPEF score. Blunted LA reverse remodeling after AF catheter ablation was also an independent predictor for higher recurrences of AF post‐1‐year AF catheter ablation.