bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Ce-Cu metal ions on Structural, Magneto-Electrical properties of Co2+ based nano ferrites and their application in Photo Catalytic activity
Authors: Yelagandula Shivakumar , Eragari Rajasheker Reddy , Gangadharaa R , Kotha Laxma Reddy , Adulla Panasa Reddy 
Published: December 2023

Abstract

Cobalt based nanoferrites Co1 − xCuxCeyFe2−yO4 Where\(x=0.0 to 0.5 with 0.1\) nano ferrites produced through citrate gel auto combustion technique and their structural and topographical studies were inspected through x-ray diffraction technique and field emission scanning electron microscope. The structural analysis confirms the prepared nano ferrites having single-phase cubic spinel, along Fd3m space group. Crystalline size of the synthesized samples found 20–26 nm calculated by Debey Scherrer formula. Catalytic activity of the samples confirmed by dye degradation studies of methylene blue and acid red dye degradation. Magnetic behavior of the materials measured by a vibrating sample magnetometer, saturation magnetization gradually decreases as increasing Cu and Ce content and found to be 26 to 18 emu/gm. Electrical properties were measured by LCR meter.
Quantitative performance assessment of Ultivue multiplex panels in formalin-fixed, paraffin-embedded human and murine tumor specimens
Authors: Ram Sripad , Mojtahedzadeh Sepideh , Aguilar Joan-Kristel , Coskran Timothy , Powell Eric , O'Neil Shawn 
Published: December 2023

Abstract

We present a rigorous validation strategy to evaluate the performance of Ultivue multiplex immunofluorescence panels. We have quantified the accuracy and precision of four different multiplex panels (three human and one mouse) in tumor specimens with varying levels of T cell density. Our results show that Ultivue panels are typically accurate wherein the relative difference in cell proportion between a multiplex image and a 1-plex image is less than 20% for a given biomarker. Ultivue panels exhibited relatively high intra-run precision (CV ≤ 20%) and relatively low inter-run precision (CV > > 20%) which can be remedied by using local intensity thresholding to gate biomarker positivity. We also evaluated the reproducibility of cell-cell distance estimates measured from multiplex images which show high intra- and inter-run precision. We introduce a new metric, multiplex labeling efficiency, which can be used to benchmark the overall fidelity of the multiplex data across multiple batch runs. Taken together our results provide a comprehensive characterization of Ultivue panels and offer practical guidelines for analyzing multiplex images.
Is the hyperdensity areas of the CT blend sign associated with the fresh bleeding in intracerebral hemorrhage?
Authors: Wu Qian , Che Wei , Chen Na , Wang Long , Ren Siying , Ye Fei , Zhao Xu , Wang Likun , Wu Guofeng 
Published: December 2023

Abstract
Background and purpose
Controversies still exist regarding the mechanism behind the formation of the CT blend sign in intracerebral hemorrhage (ICH). This study was undertaken to observe the relationship between the spot sign and the blend sign to gain insights into the blend sign's formation.
Methods
We collected patient data, including the spot sign's distribution, clinical information, and radiographic data. Patients were categorized based on the spot sign's location within the blend sign. We divided the cohort into two groups: (1) subjects with spot sign in the relatively hypodensity region of the blend sign(Hypo-spot sign group), (2) subjects with spot sign in the relatively hyperdensity region of blend sign(Hyper-spot sign group). Subsequently, based on hematoma expansion (HE), patients were grouped into either the hematoma expansion group (Expander group) or the no hematoma expansion group (No Expander group). After grouping, we analyzed the relationship between the spot sign and the blend sign, as well as the association between the blend sign and HE.
Results
A total of 205 patients were included in the study. Among them, 190 patients (92.7%) had the spot sign observed in the Hyper-spot sign group, while 55 patients (7.3%) had the spot sign identified in the Hypo-spot sign group. Significant hematoma growth was observed in 60 patients (29.3%) with HE, of which 59 patients (98.3%) had the spot sign detected in the Hyper-spot sign group, and only 1 patient (1.7%) had the spot sign found in the Hypo-spot sign group. Univariate logistic regression analysis revealed that Hyper-spot sign group (6.305, 1.810–49.072; p < 0.05) was an independent predictor of ICH growth. The sensitivity, specificity, positive predictive value, and negative predictive value of Hyper-spot sign group for predicting hematoma growth were 0.983, 0.096, 0.010, and 0.01, respectively.
Conclusions
In patients presenting both the blend sign and the spot sign, the spot sign was predominantly found in the denser region of the blend sign, indicating active bleeding in the denser part of the blend sign.
Trial registration: ClinicalTrials.gov, NCT05548530. Registered September 21, 2022,
Prognostic Analysis of Different Treatment Options for Cerebral Hemorrhage - Full Text View - ClinicalTrials.gov
Psychological and ethical issues raised by genomic in paediatric care pathway, a qualitative analysis with parents and childhood cancer patients
Authors: de Montgolfier Sandrine , Droin-Mollard Marion , Hervouet Lucile , Gimenez-Roqueplo Anne-Paule , Flahault Cécile , Petit Arnaud , Bourdeaut Franck , Julia Sophie , Rial-Sebbag Emmanuelle , Coupier Isabelle , Simaga Fatoumata , Brugieres Laurence , Guerrini-Rousseau Lea , Claret Beatrice , Cave Helene , Strullu Marion , Lahlou-Laforêt Khadija 
Published: December 2023

Abstract
In paediatric oncology, genomics raises new ethical, legal and psychological issues, as somatic and constitutional situations intersect throughout the care pathway. The discovery of potential predisposition in this context is sometimes carried out outside the usual framework. This article focuses on the views of children with cancer and their parents about their experience with genomic testing. 48 semi-structured interviews were performed with children with cancer and one of their parents, before and/or after receiving the genetic test results. The interviews were fully transcribed, coded and thematically analysed using an inductive method. This analysis revealed several themes that are key issues for parents and children: perceived understanding and consenting, apprehension about the test outcomes (expectations and fears), perception and attitude towards incidental findings. The main expectation for parents and children was an aetiological explanation. Children also emphasized the altruistic meaning of genetic testing, while parents seemed to expect a therapeutic and preventive approach for their child and the rest of the family. Parents were more concerned about a family risk, while children were more afraid of cancer relapse or transmission to their descendants. Both groups suggested possible feelings of guilt concerning family transmission and imaginary representations of what genomics may allow. Incidental findings were not understood by children, while some parents perceived the related issues and hesitated between wanting or not to know. A multidisciplinary step by step approach would be an interesting way to help parents and children to better grasp the complexity of genetic and/or genomic testing.
Association of exposure factors and their causal relationship with oral cancer: A Mendelian randomization study
Authors: Li Yunyao , Wang Kang , Li Xiaobing , Zhang Linqian 
Published: December 2023

Abstract
Objectives There is a strong association among risk factors for oral cancer (ORCA), such as smoking, alcohol consumption, fiber intake, and red meat intake. The apparent synergistic effects reported in previous observational studies may also underestimate the independent effects.Our study aims to further explore the potential etiology and causality of oral cancer.
Materials and Methods This study used the genome-wide association studies database (GWAS) for Mendelian randomization (MR) analysis to explore exposure factors associated with ORCA and detect the genetic causality between these exposures and ORCA risk.
Results Our results demonstrated that in univariate MR analysis, the five exposure factors (celery intake, average weekly beer and cider intake, spirits intake, and pork intake) were risk factors, and oily fish intake was a safety factor, but in multivariate MR analysis, pork intake had the greatest impact on oral cancer when the five food/drink intakes were simultaneously consumed.
Conclusions The causal relationship between the five exposure factors (oily fish intake, celery intake, pork intake, average weekly beer and cider intake, and spirits intake) and oral cancer was analyzed. The causal effects of pork on oral cancer may be underestimated.
Clinical Relevance Prevention of oral cancer requires better education about lifestyle-related risk factors, and improved awareness and tools for early diagnosis. Our study provides some risk factors that cannot be ignored for the cause prevention of oral cancer, such as pork intake, and its role in oral cancer prevention and control.
How to assess patient satisfaction in secondary unilateral cleft lip and palate rhinoplasty?
Authors: Fioravanti Alex Boso , Jorge Beatriz M. , Alonso Nivaldo 
Published: December 2023

Abstract
Background: This study aimed to compare two specific patient-reported outcomes (PROs) to evaluate the satisfaction and impact of secondary rhinoplasty on the quality of life of patients with cleft lip/palate (CL/P) and, determine if they are equivalent.
Design:This is a prospective study.
Setting: developed in a tertiary public hospital.
Patients: We selected patients who answered the ROE and CLEFT-Q questionnaires in pre and 12 months of postoperative surgery.
Methods: The questionnaire Rhinoplasty Outcomes Evaluation (ROE) allowed measure qualitative variables and CLEFT-Q was created for evaluation outcomes of appearance, facial function, and health-related quality of life (HR-QOL), however they are not equivalent.
Results: Eighteen patients participated in our study with an average old of 21.34±4.60 years. All patients have their scores improved in the ROE and CLEFT-Q questionnaires. The mean ROE pre-operatively score was 22.4±12.2 and postoperatively was 70.37±17.9 (p<0.05). The overall aesthetics concepts for CLEFT-Q pre-operatively score was 30.08±12.1 and the mean score post-operatively was 65.5±17.9 (p<0.05).
Conclusions: ROE and CLEFT-Q questionnaires are two useful tools for assessing the outcomes and quality of life after secondary rhinoseptoplasty surgery in CL/P. This surgery significantly improved patient quality of life regarding nose function and appearance. CLEFT-Q does not measure nasal airway function as ROE and for that reason, we suggest that some questions in this area be added to this one, making it complete. The scales used have similar accuracy and can be used to measure the satisfaction of patients with CL/P.
Trial Registration: This study was registered on the Brazilian clinical trials website (https://ensaiosclinicos.gov.br/rg/RBR-4wyfw3k), with the number U1111-1299-0385.
Extant and extinct bilby genomes combined with Indigenous knowledge improve conservation of a unique Australian marsupial
Authors: Hogg Carolyn , Edwards Richard , Farquharson Katherine , Silver Luke , Brandies Parice , Peel Emma , Escalona Merly , Jaya Frederick , Sun Rujiporn , Batley Kimberley , Bradford Tessa , Chang J King , Chen Zhiliang , Deshpande Nandan , Dziminski Martin , Ewart Kyle , Griffith Oliver , Marin-Gual Laia , Moon Katherine , Travouillon Kenny , Waters Paul , Whittington Camilla , Wilkins Marc , Helgen Kristofer , Lo Nathan , Ho Simon , Ruiz-Herrera Aurora , Paltridge Rachel , Graves Jennifer , Renfree Marilyn , Shapiro Beth , Ottewell Kym , Rangers Kiwirrkurra Community , Belov Katherine 
Published: December 2023

Abstract

The Ninu (Greater bilby, Macrotis lagotis) is a desert-dwelling, culturally and ecologically important marsupial. In collaboration with Indigenous rangers and conservation managers, we generated the first Ninu chromosome-level genome assembly (3.66 Gbp) and genome sequences for the extinct Yallara (lesser bilby, Macrotis leucura) to understand their biology and guide Ninu conservation management. Resequenced genomes (temperate Ninu=6; semi-arid Ninu=6; Yallara=4) revealed two major population crashes during global cooling events. Despite their 45-year long captive history, Ninu have fewer long runs of homozygosity than other larger mammals, which may be attributable to their boom-bust life-history. We assessed the beneficial impact of targeted conservation actions, like translocations, in the contemporary metapopulation (N=363 Ninu) and designed a SNP panel used by Indigenous rangers in monitoring remote wild populations. We dig into the unique Ninu biology using 12 tissue transcriptomes revealing expression of all 115 conserved eutherian chorioallantoic placentation genes in the uterus; an XY1Y2 sex chromosome system generated by fusion of the X with a large telocentric autosome; genes involved in anatomical and metabolic pathway adaptations to aridity; and expansions in olfactory receptor genes. Together, we demonstrate the holistic value of genomics in addressing key Indigenous and conservation management questions for ongoing conservation efforts.
Communities and Data Revolution
Authors: Vahabi Fataneh 
Published: December 2023

Abstract
The data revolution is transforming the way we interact with the world. With the rise of big data, organizations have access to an unprecedented amount of data, enabling them to better inform decision-making and develop new products and services. However, to capitalize on this data revolution, it is essential to build strong, connected communities to make full use of the data. By engaging stakeholders and developing a diverse and engaged community, organizations can better understand how to use data to gain insights, create new opportunities, and solve problems. This includes engaging with citizens, businesses, civil society organizations, and local government to ensure a wide range of perspectives are heard and incorporated into the data strategy. This kind of engagement also provides a platform for public dialogue and building trust in the data-driven decision-making process. Organizations should also invest in data literacy initiatives to ensure that data is used effectively. These initiatives should be tailored to the needs of the community and focus on educating stakeholders on the responsible use of data. This could include workshops, training sessions, and hackathons to familiarize the community with data analysis, data visualization, and data storytelling. Finally, organizations should develop data sharing protocols to ensure that data is shared securely and ethically. This includes having clear terms and conditions regarding data usage, data protection, and data security. It also involves developing policies to ensure that data is used responsibly and in the public interest.
Hydrogeological characterization and extended seasonal hydrogeochemical monitoring of a CO₂ controlled-release experimental site: the case of TECNOPUC-Viamão, Rio Grande do Sul state, Southern Brazil
Authors: Zielinski João Pedro Tauscheck , Hamerski Fernando , Vecchia Felipe Dalla , Melo Clarissa Lovato , Reginato Pedro Antônio Roehe 
Published: December 2023

Abstract

Reduced-scale CO2 release experiments in shallow aquifers serve as crucial monitoring strategies for detecting unintended CO2 leakage into potable aquifers within Carbon Capture and Storage (CCS) projects. Understanding site-specific geological, hydrogeological, and climatic features is essential. However, accurately tracing changes in groundwater quality due to this process and using hydrochemical parameters for CO2 leakage diagnosis require establishing a solid, seasonally relevant baseline to avoid misinterpretation. This study focuses on detailing the geological, hydrogeological, and geophysical characteristics of the TECNOPUC-Viamão CO2 controlled-release experimental site in Rio Grande do Sul, Brazil. It presents a comprehensive three-year investigation into the seasonal natural background hydrochemistry. Field characterization involved recognition boreholes, in-situ infiltration and Slug Tests, topographic surveys, DC resistivity measurements, and groundwater sampling campaigns for physicochemical, major, minor, trace elements, and δ¹³C-DIC evaluation. Results indicate the area comprises a granite-derived multilayer phreatic aquifer with two distinct hydrostratigraphic units (St and Aa). These units differ in lithological composition, hydraulic conductivities (St: 10− 4 m/s, Aa: 10− 8 m/s), apparent resistivities, and physicochemical and hydrochemical compositions. The St unit shows slightly neutral pH, higher temperature, EC, ORP, DO, Ca, Mg, K, Fe, Mn, Sr, B, HCO3−, and DIC concentrations, with δ¹³C-DIC between − 3 to -8‰. Conversely, the Aa unit displays slightly acidic pH, lower temperature, EC, ORP, higher DO fluctuation, Na, SO42−, Cl−, NO3−, Zn, Al, Ni concentrations, lower HCO3−, DIC levels, with δ¹³C-DIC ranging between − 6 to -11‰. Additionally, seasonal monitoring campaign revealed that there is a clear temperature-related influence on Ca, Mg, K, Na, NO₃⁻, and trace elements (Fe, Ba, Sr, Mn, Al, B, Ni, and Zn) behavior, which could impact further interpretation of the results during the upcoming CO2 injection phase, and that the Control Charts can confidently serve as a valuable tool in understanding the inherent natural hydrochemical trends.
Associations of rare variants in the AKAP11 gene with bipolar disorder in Chinese population
Authors: Zhang Yankai , Yu Hui , Wang Tingting , Sun Ping 
Published: December 2023

Abstract

Purpose
This pioneering study aimed to explore the associations between the A-kinase anchoring protein 11 (AKAP11) gene and bipolar disorder (BD) in a Chinese population. We sought to replicate findings from European populations regarding ultra-rare protein-truncating variants (PTVs) within exon 8 of AKAP11 and identify any novel rare mutations linked to Chinese BD patients.
Methods
We conducted a case-control association study, including a cohort of 284 Chinese BD patients, with the control group comprising 10,588 individuals from the China Metabolic Analytics Project (ChinaMAP) database. Polymerase chain reaction (PCR) amplification and Sanger sequencing were performed to analyze exon 8 of the AKAP11 gene. Statistical analysis involved chi-square tests to assess differences in allele frequency between BD patients and the control group.
Results
In our 284 Chinese BD patients, within exon 8 of the AKAP11 gene we did not find any ultra-rare PTVs previously identified in European BD patients. However, five additional rare variants were discovered, including three missense variants and two synonymous variants. Among these variants, one synonymous variant, g.42300171T > C (i.e., rs771987690), had not been reported in the ChinaMAP database. Statistical analysis did not reveal significant differences in allele frequencies between BD patients and controls (P = 0.240), but there was a noticeable trend suggesting a potential association between the rare variants with the AKAP11 gene and risk of BD. Additionally, three of the five rare variants were not documented in the Bipolar Exomes Browser (BipEx) database, the frequencies of the other two were mildly lower in cases than controls, contrary to the trend observed in the Chinese population. The observed difference may be due to population genetic-environmental interaction.
Conclusions
Our preliminary data indicates a potential trend between the AKAP11 gene and BD patients in China, despite did not reach nominal significance, calling for further analysis in a larger sample set.
Development of a smartphone screening test for preclinical Alzheimer's disease and validation across the dementia continuum
Authors: Alty Jane , Goldberg Lynette R. , Roccati Eddy , Lawler Kate , Bai Quan , Huang Guan , Bindoff Aidan D. , Li Renjie , Wang Xinyi , St George Rebecca , Rudd Kaylee , Bartlett Larissa , Collins Jessica , Aiyede Mimieveshiofuo , Fernando Nadeeshani , Bhagwat Anju , Giffard Julia , Salmon Katharine , McDonald Scott , King Anna , Vickers James 
Published: December 2023

Abstract
Background
Dementia prevalence is predicted to triple to 152 million globally by 2050. Alzheimer's disease (AD) constitutes 70% of cases. There is an urgent need to identify individuals with preclinical AD (a 10–20-year period of progressive brain pathology without noticeable cognitive symptoms) for targeted risk reduction. Current tests of AD pathology are either too invasive, specialised or expensive for population-level assessments. Cognitive tests are normal in preclinical AD. Emerging evidence demonstrates that movement analysis is sensitive to AD across the disease continuum, including preclinical AD. Our new smartphone test, TapTalkTest combines analysis of hand and speech-like movements to detect AD risk. This study aims to (1) determine which combinations of hand-speech movement data most accurately predict preclinical AD, (2) determine usability, reliability, and validity of TapTalkTest in cognitively asymptomatic older adults and, (3) prospectively validate TapTalkTest in older adults who have cognitive symptoms against cognitive tests and clinical diagnoses of Mild Cognitive Impairment and AD dementia.
Methods
Aim 1 will be addressed in a cross-sectional study of 500 cognitively asymptomatic older adults who will complete computerised tests comprising measures of hand motor control (finger tapping) and oro-motor control (syllabic diadochokinesis). Motor measures will be compared to a blood-based AD biomarker, phosphorylated tau 181 to develop an algorithm that classifies preclinical AD risk.
Aim 2 comprises three sub-studies in cognitively asymptomatic adults: (i) a cross-sectional study of 30-40 adults to determine the validity of data collection from different types of smartphones, (ii) a prospective cohort study of 50-100 adults ≥50 years old to determine usability and test-retest reliability, and (iii) a prospective cohort study of ~1,000 adults ≥50 years old to validate against cognitive measures.
Aim 3 will be addressed in a cross-sectional study of ~200 participants with cognitive symptoms to validate TapTalkTest against Montreal Cognitive Assessment and interdisciplinary consensus diagnosis.
Discussion
This study will establish the precision of TapTalkTest to identify preclinical AD and estimate risk of cognitive decline. If accurate, this innovative smartphone app will enable low-cost, accessible screening of individuals for AD risk. This will have wide applications in public health initiatives and clinical trials.
Trial registration: ClinicalTrials.gov identifier: NCT06114914, 29 October 2023. Retrospectively registered.