bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eco‐friendly micellar HPLC approach for simultaneous estimation of combination therapy for hidradenitis suppurativa: Applications to spiked human plasma and different dosage forms
Authors: Taha Asmaa M. , Hassan Wafaa S. , Elmasry Manal S. , Sayed Rania A. 
Published: November 2023

AbstractThis study introduces a new method for analyzing rifampicin, moxifloxacin, and metronidazole using a green micellar High Performance Liquid Chromatography‐Ultraviolet method in bulk drugs, different commercial formulations, and spiked human plasma. The combined therapy of these three broad‐spectrum antibiotics is used to cure refractory hidradenitis suppurativa (HS), an inflammatory condition affecting the skin. The sustainable separation was attained on a reversed‐phase C18 Kinetex® column maintained at ambient temperature in less than 5 min. The mobile phase comprises 0.1 M sodium dodecyl sulfate (SDS) in water, pH 3.5, adjusted using o‐phosphoric acid, and 10% n‐butanol. The flow rate was 1 mL/min, with 10 μL injection volume and UV detection at 230 nm. The impact of three key significant variables, SDS concentration, n‐butanol percentage, and the mobile phase pH, on suitability parameters was studied. ICH and FDA guidelines were committed to when validating the technique. The results showed linear calibration graphs with high precision and accuracy, in both pure and spiked plasma. The method is efficient, easy to use, and has a high sample throughput, making it suitable for routine analysis in the quality control department and therapeutic monitoring. It is also evaluated as a green‐and‐white substitute for traditional reported methods.
Demystifying dimensionality reduction techniques in the ‘omics’ era: A practical approach for biological science students
Authors: Garma Leonardo D. , Osório Nuno S. 
Published: November 2023

AbstractDimensionality reduction techniques are essential in analyzing large ‘omics’ datasets in biochemistry and molecular biology. Principal component analysis, t‐distributed stochastic neighbor embedding, and uniform manifold approximation and projection are commonly used for data visualization. However, these methods can be challenging for students without a strong mathematical background. In this study, intuitive examples were created using COVID‐19 data to help students understand the core concepts behind these techniques. In a 4‐h practical session, we used these examples to demonstrate dimensionality reduction techniques to 15 postgraduate students from biomedical backgrounds. Using Python and Jupyter notebooks, our goal was to demystify these methods, typically treated as “black boxes”, and empower students to generate and interpret their own results. To assess the impact of our approach, we conducted an anonymous survey. The majority of the students agreed that using computers enriched their learning experience (67%) and that Jupyter notebooks were a valuable part of the class (66%). Additionally, 60% of the students reported increased interest in Python, and 40% gained both interest and a better understanding of dimensionality reduction methods. Despite the short duration of the course, 40% of the students reported acquiring research skills necessary in the field. While further analysis of the learning impacts of this approach is needed, we believe that sharing the examples we generated can provide valuable resources for others to use in interactive teaching environments. These examples highlight advantages and limitations of the major dimensionality reduction methods used in modern bioinformatics analysis in an easy‐to‐understand way.
Staging Parkinson's disease according to the MNCD classification correlates with caregiver burden
Authors: Santos‐García Diego , de Deus Fonticoba Teresa , Cores Bartolomé Carlos , Feal Painceiras María J. , García Díaz Iago , Alvarado María Cristina Íñiguez , Paz Jose Manuel , Jesús Silvia , Cosgaya Marina , Caldentey Juan García , Caballol Nuria , Legarda Ines , Hernández Vara Jorge , Cabo Iria , López Manzanares Lydia , González Aramburu Isabel , Ávila Rivera Maria A. , Gómez Mayordomo Víctor , Nogueira Víctor , Dotor García‐Soto Julio , Borrué Carmen , Solano Vila Berta , Álvarez Sauco María , Vela Lydia , Escalante Sonia , Cubo Esther , Mendoza Zebenzui , Martínez Castrillo Juan C. , Sánchez Alonso Pilar , Alonso Losada Maria G. , López Ariztegui Nuria , Gastón Itziar , Kulisevsky Jaime , Seijo Manuel , Valero Caridad , Alonso Redondo Ruben , Buongiorno Maria Teresa , Ordás Carlos , Menéndez‐González Manuel , McAfee Darrian , Martinez‐Martin Pablo , Mir Pablo ,   COPPADIS Study Group
Published: November 2023

AbstractBackground and objectiveRecently, we demonstrated that staging Parkinson's disease (PD) with a novel simple classification called MNCD, based on four axes (motor, non‐motor, cognition, and dependency) and five stages, correlated with disease severity and patients’ quality of life. Here, we analyzed the correlation of MNCD staging with PD caregiver's status.Patients and methodsData from the baseline visit of PD patients and their principal caregiver recruited from 35 centers in Spain from the COPPADIS cohort from January 2016 to November 2017 were used to apply the MNCD total score (from 0 to 12) and MNCD stages (from 1 to 5) in this cross‐sectional analysis. Caregivers completed the Zarit Caregiver Burden Inventory (ZCBI), Caregiver Strain Index (CSI), Beck Depression Inventory‐II (BDI‐II), PQ‐10, and EUROHIS‐QOL 8‐item index (EUROHIS‐QOL8).ResultsTwo hundred and twenty‐four PD patients (63 ± 9.6 years old; 61.2% males) and their caregivers (58.5 ± 12.1 years old; 67.9% females) were included. The frequency of MNCD stages was 1, 7.6%; 2, 58.9%; 3, 31.3%; and 4–5, 2.2%. A more advanced MNCD stage was associated with a higher score on the ZCBI (p < .0001) and CSI (p < .0001), and a lower score on the PQ‐10 (p = .001), but no significant differences were observed in the BDI‐II (p = .310) and EUROHIS‐QOL8 (p = .133). Moderate correlations were observed between the MNCD total score and the ZCBI (r = .496; p < .0001), CSI (r = .433; p < .0001), and BDI‐II (r = .306; p < .0001) in caregivers.ConclusionStaging PD according to the MNCD classification is correlated with caregivers’ strain and burden.
The fate of coronary dissections left after sirolimus‐coated balloon angioplasty: A prespecified subanalysis of the EASTBOURNE study
Authors: El Khoury Antoine , Lazar Leontin , Cortese Bernardo 
Published: November 2023

AbstractObjectivesWe sought to understand the clinical outcomes of dissections left untreated after sirolimus drug‐coated balloon (DCB) angioplasty.BackgroundDCB may be a valuable alternative to stents for the treatment of native coronary lesions, but the risk of having a dissection after DCB‐angioplasty is not negligible. While type A and B dissections can be safely treated conservatively, some debate exists regarding type C dissections. We previously showed the safety of dissections left untreated after second‐generation paclitaxel‐DCB. However, the fate of dissections after sirolimus‐DCB angioplasty has not been investigated so far.MethodsEASTBOURNE is a prospective, multicenter, international, investigator‐driven study aiming to explore the safety and efficacy of a novel sirolimus‐DCB. This study enrolled a consecutive, all‐comer population of coronary artery disease patients and is the largest prospective study on DCB so far. Primary endpoints of the study, target‐lesion revascularization (TLR), and other clinical endpoints at 12 months, have been presented elsewhere. This is a prespecified subgroup analysis of the patients left with not‐flow limiting dissection after DCB angioplasty, with complete 12 months follow‐up and comparison between patients left with a dissection versus patients with DCB used for de novo lesions.ResultsBetween September 2016 and November 2020, a total of 2123 patients were enrolled at 38 study centers. Seventy‐three patients were left with nonflow limiting dissections (43 type A, 27 type B, 3 type C) and underwent complete 1‐year clinical follow‐up. In the nondissection group, 1110 patients had de‐novo coronary artery disease while 900 had in‐stent restenosis. Baseline characteristics were similar between the groups, while the dissection group was associated with longer lesions (23.8 vs. 18.4 mm, p < 0.001) and more frequent use of predilation (100 vs. 91.4%, p = 0.016). At 12‐month follow‐up, no significant differences among the groups were found, with a total of 1.25% TLR in the dissection cohort versus 5.6% in the de‐novo cohort (p = 0.13), and an overall rate of major adverse cardiovascular events of 4.4% versus 10.1% (p = 0.18). Total death (1.5 vs. 2.6, p = 0.87), cardiac death, myocardial infarction (0% vs. 2.5%, p = 0.35), and bleedings did not differ significantly among the groups as well.ConclusionsIn this subgroup analysis of the EASTBOURNE study of consecutive patients treated with new‐generation sirolimus DCB, dissections left untreated after angioplasty did not lead to an increase in adverse events.
Fractional flow reserve versus intravascular imaging to guide decision‐making for percutaneous coronary intervention in intermediate lesions: A meta‐analysis
Authors: Liu Xiaochen , Zhang Yushi , Xu Yang , Tang Zhe , Wang Xiao , Nie Shaoping , Mintz Gary S. 
Published: November 2023

AbstractBackgroundBoth fractional flow reserve (FFR) and intravascular imaging (IVI) have been used to guide the decision‐making for percutaneous coronary intervention (PCI) in intermediate coronary stenosis. Nevertheless, studies that directly compared the prognostic significance of these two strategies are scarce.AimsThe aim of this meta‐analyses was to evaluate the impact of FFR versus IVI to guide the decision‐making in PCI for intermediate stenosis on clinical outcomes.MethodsWe systematically searched PubMed, Embase, Cochrane, and relevant database from inception date to September 2022 for observational studies and randomized clinical trials (RCTs) which compared FFR and IVI‐based decision‐making in PCI for intermediate stenosis. The primary outcome was a composite of major adverse cardiac event (MACE). Pooled risk ratios (RR) were calculated using random effects models and heterogeneity were evaluated with the I2 statistic.ResultsWe identified 5 studies (3 RCTs and 2 observational studies) with 3208 patients. The follow‐up duration ranged from 12 to 24 months. Among five studies, four compared FFR with intravascular ultrasound while one compared FFR with optical coherence tomography. There was no statistically difference between FFR and IVI in the incidence of MACE (RR: 1.19; 95% confidence interval: 0.85–1.68; p = 0.31) and its individual components. These results were consistent regardless of various cut‐off value of PCI across the studies. Compared with IVI, FFR was associated with a lower PCI rate (37.0% vs. 60.3%; p < 0.001).ConclusionsThe decision to perform PCI for intermediate stenosis guided by FFR or IVI showed a similar clinical outcome. The use of FFR significantly reduced the need for PCI.
Proteomic prediction of incident heart failure and its main subtypes
Authors: Emilsson Valur , Jonsson Brynjolfur G. , Austin Thomas R. , Gudmundsdottir Valborg , Axelsson Gisli T. , Frick Elisabet A. , Jonmundsson Thorarinn , Steindorsdottir Anna E. , Loureiro Joseph , Brody Jennifer A. , Aspelund Thor , Launer Lenore J. , Thorgeirsson Gudmundur , Kortekaas Kirsten A. , Lindeman Jan H. , Orth Anthony P. , Lamb John R. , Psaty Bruce M. , Kizer Jorge R. , Jennings Lori L. , Gudnason Vilmundur 
Published: November 2023

AbstractAimTo examine the ability of serum proteins in predicting future heart failure (HF) events, including HF with reduced or preserved ejection fraction (HFrEF or HFpEF), in relation to event time, and with or without considering established HF‐associated clinical variables.Methods and resultsIn the prospective population‐based Age, Gene/Environment Susceptibility Reykjavik Study (AGES‐RS), 440 individuals developed HF after their first visit with a median follow‐up of 5.45 years. Among them, 167 were diagnosed with HFrEF and 188 with HFpEF. A least absolute shrinkage and selection operator regression model with non‐parametric bootstrap were used to select predictors from an analysis of 4782 serum proteins, and several pre‐established clinical parameters linked to HF. A subset of 8–10 distinct or overlapping serum proteins predicted different future HF outcomes, and C‐statistics were used to assess discrimination, revealing proteins combined with a C‐index of 0.80 for all incident HF, 0.78 and 0.80 for incident HFpEF or HFrEF, respectively. In the AGES‐RS, protein panels alone encompassed the risk contained in the clinical information and improved the performance characteristics of prediction models based on N‐terminal pro‐B‐type natriuretic peptide and clinical risk factors. Finally, the protein predictors performed particularly well close to the time of an HF event, an outcome that was replicated in the Cardiovascular Health Study.ConclusionA small number of circulating proteins accurately predicted future HF in the AGES‐RS cohort of older adults, and they alone encompass the risk information found in a collection of clinical data. Incident HF events were predicted up to 8 years, with predictor performance significantly improving for events occurring less than 1 year ahead, a finding replicated in an external cohort study.
Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation
Authors: Rosarda Jessica D. , Stanton Caroline R. , Chen Emily B. , Bollong Michael J. , Wiseman R. Luke 
Published: November 2023

AbstractThe NLRP3 inflammasome is a cytosolic protein complex that regulates innate immune signaling in response to diverse pathogenic insults through the proteolytic processing and secretion of pro‐inflammatory cytokines such as IL‐1β. Hyperactivation of NLRP3 inflammasome signaling is implicated in the onset and pathogenesis of numerous diseases, motivating the discovery of new strategies to suppress NLRP3 inflammasome activity. We sought to define the potential for the proteostasis regulator AA147 to inhibit the assembly and activation of the NLRP3 inflammasome. AA147 is a pro‐drug that is metabolically converted to a reactive metabolite at the endoplasmic reticulum (ER) membrane to covalently modify ER‐localized proteins such as protein disulfide isomerases (PDIs). We show that AA147 inhibits NLRP3 inflammasome activity in monocytes and monocyte‐derived macrophages through a mechanism involving impaired assembly of the active inflammasome complex. This inhibition is mediated through AA147‐dependent covalent modification of PDIA1. Genetic depletion or treatment with other highly selective PDIA1 inhibitors similarly blocks NLRP3 inflammasome assembly and activation. Our results identify PDIA1 as a potential therapeutic target to mitigate NLRP3 inflammasome‐mediated pro‐inflammatory signaling implicated in etiologically diverse diseases.
Caregiver involvement in applied behavior‐analytic research: A scoping review and discussion
Authors: Becraft Jessica L. , Hardesty Samantha L. , Goldman Kissel J. , Shawler Lesley A. , Edelstein Matthew L. , Orchowitz Phillip 
Published: November 2023

AbstractWe conducted a scoping review to characterize the role of caregiver involvement in behavior‐analytic research. We reviewed eight behavioral‐learning journals from 2011–2022 for works that included children or caregivers as participants and characterized caregiver involvement as passive (implications for caregivers, input, social validity) and active (implementation, caregiver behavior, training, caregiver‐collected data). The review identified 228 studies, and almost all (96.1%; n = 219) involved caregivers in some capacity; 94.3% (n = 215) had passive involvement (26.8% had only passive involvement; n = 61), 69.3% (n = 158) had active involvement (1.8% had only active involvement; n = 4), and 3.9% (n = 9) had neither passive nor active involvement. Involvement generally increased over publication years. The most common types of involvement were implications for caregivers, implementation, and input; caregiver‐collected data were rare. We propose considerations when engaging caregivers in research and suggest new avenues of inquiry related to caregivers' treatment objectives and social validity, treatment implementers, and caregiver‐collected data.
Long‐Term Krill Oil Administration Alleviated Early Mild Cognitive Impairment in APP/PS1 Mice
Authors: Du Yufeng , Song Lin , Dong Xiufang , Li Hongyan , Xie Wancui , Wang Yuming , Che Hongxia 
Published: November 2023

ScopeAlzheimer's disease is an age‐dependent neurodegenerative disorder. Mounting studies focus on the improvement of advanced cognitive impairment by dietary nutrients. Krill oil (KO), a rich source of DHA/EPA and astaxanthin, is effective in improving cognitive function. The study mainly investigates the protective effects of long‐term KO administration on early cognitive impairment.Methods and resultsResults show that 2 months KO administration (50 and 100 mg kg−1 BW) can dramatically promote learning and memory abilities. Mechanism studies demonstrate that KO reduces amyloid β concentration by regulating the amyloidogenic pathway, inhibits neuro‐inflammation via regulating TLR4‐NLRP3 signaling pathway, and prevents neuron injure. KO supplementation also enhances gut barrier integrity, reduces serum lipopolysaccharide leakage, and alters the gut microbiota by reducing Helicobacteraceae, Lactobacillaceae proportion, increasing Dubosiella and Akkermansia relative abundance. Particularly, a significant increase of isovaleric acid, propionic acid, and acetic acid levels is observed after KO supplementation. Correlation analysis shows that short‐chain fatty acids (SCFAs), gut microbiota, and cognitive function are strongly correlated.ConclusionsThe results reveal that KO relieves early mild cognitive impairment possibly for its role in mediating the gut microbiome‐SCFAs‐brain axis. Thus, KO may provide potential intervention strategies to prevent cognitive impairment in the early stages through the microbiota‐gut‐brain axis.
Postoperative Outcomes of Intracapsular Tonsillectomy With Coblation: A Systematic Review and Meta‐Analysis
Authors: Lin Huiying , Hajarizadeh Behzad , Wood Andrew James , Selvarajah Kumanan , Ahmadi Omid 
Published: November 2023

AbstractObjectiveFollowing tonsillectomy, postoperative pain and hemorrhage from the tonsillar bed are causes of significant morbidity. Intracapsular tonsillectomy with Coblation is suggested to minimize such morbidity while remaining efficacious in long‐term outcomes.This systematic review and meta‐analysis assessed short‐term morbidity and long‐term outcomes from intracapsular tonsillectomy with Coblation, focusing primarily on posttonsillectomy hemorrhage.Data SourcesMedline, Embase, and the Cochrane Library.Review MethodsGuided by PRISMA guidelines, studies on intracapsular tonsillectomy with Coblation published between December 2002 and July 2022 evaluating frequency of posttonsillectomy hemorrhage were screened. Studies without primary data were excluded. Meta‐analysis was conducted using the random‐effect model. The primary outcome was the proportion of patients who experienced posttonsillectomy hemorrhage. The secondary outcomes were posttonsillectomy pain, the proportion requiring revision tonsillectomy, and severity of sleep‐disordered breathing measured by polysomnography outcomes.ResultsFrom 14 studies there were 9821 patients. The proportion of total posttonsillectomy hemorrhage was 1.0% (95% confidence interval [CI] 0.5%‐1.6%, n = 9821). The proportion experiencing primary hemorrhage, secondary hemorrhage, and those requiring further tonsil surgery were 0.1% (95% CI 0.0%‐0.1%; study n = 7), 0.8% (95% CI 0.2%‐1.4%; study n = 7), and 1.4% (95% CI 0.6%‐2.2%; study n = 6), respectively. Mean reduction in apnea‐hypopnea index was –16.0 events per hour (95% CI −8.8 to −23.3, study n = 3) and mean increase in oxygen nadir was 5.9% (95% CI 2.6%‐9.1%, study n = 3).ConclusionIntracapsular tonsillectomy with Coblation has been demonstrated to have a low rate of posttonsillectomy hemorrhage. Data regarding long‐term tonsil regrowth and need for reoperation were encouraging of the efficacy of this technique.
Respiratory culture growth and 3‐years lung health outcomes in children with bronchopulmonary dysplasia and tracheostomies
Authors: Steuart Rebecca , Pan Amy Y. , Woolums Abigail , Benscoter Dan , Russell Christopher J. , Henningfeld Jennifer , Thomson Joanna 
Published: November 2023

AbstractBackgroundWhile bacteria identification on respiratory cultures is associated with poor short‐term outcomes in children with bronchopulmonary dysplasia (BPD) and tracheostomies, the influence on longer‐term respiratory support needs remains unknown.ObjectiveTo determine if respiratory culture growth of pathogenic organisms is associated with ongoing need for respiratory support, decannulation, and death at 3 years posttracheostomy placement in children with BPD and tracheostomies.MethodsThis single center, retrospective cohort study included infants and children with BPD and tracheostomies placed 2010–2018 and ≥1 respiratory culture obtained in 36 months posttracheostomy. Primary predictor was any pathogen identified on respiratory culture. Additional predictors were any Pseudomonas aeruginosa and chronic P. aeruginosa identification. Outcomes included continued use of respiratory support (e.g., oxygen, positive pressure), decannulation, and death at 3 years posttracheostomy. We used Poisson regression models to examine the relationship between respiratory organisms and outcomes, controlling for patient‐level covariates and within‐patient clustering.ResultsAmong 170 children, 59.4% had a pathogen identified, 28.8% ever had P. aeruginosa, and 3.5% had chronic P. aeruginosa. At 3 years, 33.1% of alive children required ongoing respiratory support and 24.8% achieved decannulation; 18.9% were deceased. In adjusted analysis, any pathogen and P. aeruginosa were not associated with ongoing respiratory support or mortality. However, P. aeruginosa was associated with decreased decannulation probability (adjusted risk ratio 0.48, 95% CI 0.23–0.98). Chronic P. aeruginosa was associated with lower survival probability.ConclusionOur findings suggest that respiratory pathogens including P. aeruginosa may not promote long‐term respiratory dysfunction, but identification of P. aeruginosa may delay decannulation.
Social‐ecological systems approach for adaptation to climate change
Authors: Hossain Md Sarwar , Basak Sayantani M. , Amin Md Nurul , Anderson Carl C. , Cremin Emilie , Renaud Fabrice G. 
Published: November 2023

AbstractAdaptation to climate change has become part of national development programs (e.g., National Adaptation Planning). Though communities are adapting to climate change across the world, this perspective argues that understanding social and ecological systems requires greater attention to enhance resilience for achieving and moving beyond the Sustainable Development Goals (SDGs) in response to climate change across the world. In addition, based on a systematic literature review, this perspective identifies key research gaps and addresses relevant key challenges and future research direction for social and ecological systems (SES) adaptation to climate change. Ultimately, this may also help move from the existing limits to adaptation (intolerable risks through adaptive actions) concept to social‐ecological limits to adaptation and offers an opportunity to integrate adaptation to climate change in development planning by considering both SES.