bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of Distinct Bladder Pain Phenotypes Utilizing the MAPP Research Network Cohort
Authors: Nettey Oluwarotimi Sewedo , Gu Cindy , Jackson Nicholas James , Ackerman A. Lenore 
Published: February 2024

Abstract
Introduction and Hypothesis
As interstitial cystitis/bladder pain syndrome (IC/BPS) likely represents multiple pathophysiologies, we sought to validate three clinical phenotypes of IC/BPS patients in a large, multi-center cohort using unsupervised machine learning (ML) analysis.

Methods
Using the female Genitourinary Pain Index and O’Leary-Sant Indices, k-means unsupervised clustering was utilized to define symptomatic phenotypes in 130 premenopausal IC/BPS participants recruited through the Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) research network. Patient-reported symptoms were directly compared between MAPP ML-derived phenotypic clusters to previously defined phenotypes from a single center (SC) cohort.

Results
Unsupervised ML categorized IC/BPS participants into three phenotypes with distinct pain and urinary symptom patterns: myofascial pain, non-urologic pelvic pain, and bladder-specific pain. Defining characteristics included presence of myofascial pain or trigger points on examination for myofascial pain patients (p = 0.003) and bladder pain/burning for bladder-specific pain patients (p < 0.001). The three phenotypes were derived using only 11 features (fGUPI subscales and ICSI/ICPI items), in contrast to 49 items required previously. Despite substantial reduction in classification features, unsupervised ML independently generated similar symptomatic clusters in the MAPP cohort with equivalent symptomatic patterns and physical examination findings as the SC cohort.

Conclusions
The reproducible identification of IC/BPS phenotypes, distinguishing bladder-specific pain from myofascial and genital pain, using independent ML analysis of a multicenter database suggests these phenotypes reflect true pathophysiologic differences in IC/BPS patients.
Entanglement Monogamy via Multivariate Trace Inequalities
Authors: Berta Mario , Tomamichel Marco 
Published: February 2024

AbstractEntropy is a fundamental concept in quantum information theory that allows to quantify entanglement and investigate its properties, for example its monogamy over multipartite systems. Here, we derive variational formulas for relative entropies based on restricted measurements of multipartite quantum systems. By combining these with multivariate matrix trace inequalities, we recover and sometimes strengthen various existing entanglement monogamy inequalities. In particular, we give direct, matrix-analysis-based proofs for the faithfulness of squashed entanglement by relating it to the relative entropy of entanglement measured with one-way local operations and classical communication, as well as for the faithfulness of conditional entanglement of mutual information by relating it to the separably measured relative entropy of entanglement. We discuss variations of these results using the relative entropy to states with positive partial transpose, and multipartite setups. Our results simplify and generalize previous derivations in the literature that employed operational arguments about the asymptotic achievability of information-theoretic tasks.
Optimal Gevrey Regularity for Supercritical Quasi-Geostrophic Equations
Authors: Li Dong 
Published: February 2024

AbstractWe consider the two dimensional surface quasi-geostrophic equations with super-critical dissipation. For large initial data in critical Sobolev and Besov spaces, we prove optimal Gevrey regularity endowed with the same decay exponent as the linear part. This settles several open problems in Biswas (J Differ Equ 257(6):1753–1772, 2014), Biswas et al. (J Funct Anal 269(10):3083–3119, 2015).
Osteosarcopenia: Prevalence and 10-Year Fracture and Mortality Risk – A Longitudinal, Population-Based Study of 75-Year-Old Women
Authors: Paulin Tine Kolenda , Malmgren Linnea , McGuigan Fiona E , Akesson Kristina E 
Published: February 2024

AbstractOsteosarcopenia is the coexistence of low bone mass and sarcopenia. In older women, its prevalence is not well described, and it is unknown if sarcopenia is additive to low bone mass for fracture and mortality risk. The study investigated prevalence of osteosarcopenia and if osteosarcopenia is associated with higher fracture and mortality risk than low bone mass alone in older community-dwelling women. The longitudinal, population-based OPRA Cohort (n = 1044), all aged 75 at inclusion, followed for 10 years. Using WHO and EWGSOP2 definitions for low bone mass (T-score < −1.0 femoral neck) and sarcopenia (knee strength; appendicular lean muscle mass) women were categorized (1) Normal, (2) Low bone mass (LBM), and 3) Osteosarcopenia (probable; confirmed). Risk of hip, major osteoporotic fracture, and mortality were estimated. Osteosarcopeniaconfirmed prevalence increased from age 75 to 80 and 85 from 3.0% (29/970) to 4.9% (32/656) to 9.2% (33/358) but prevalence is potentially 2–4 times higher (11.8%, 13.4%, 20.3%) based on osteosarcopeniaprobable. Having osteosarcopeniaprobable significantly increased 10-year risk of hip fracture (HRadj 2.67 [1.34–5.32]), major osteoporotic fracture (HRadj 2.04 [1.27–3.27]), and mortality (HRadj 1.91 [1.21–3.04]). In contrast, LBM increased osteoporotic fracture risk (HRadj 2.08 [1.46–2.97], but not hip fracture (HRadj 1.62 [0.92–2.85]) or mortality (HRadj 0.94 [0.64–1.38]). Median time-to-hip fracture was 7.6 years (normal), 6.0 years (LBM), and 5.7 years (osteosarcopeniaprobable). Prevalence of confirmed osteosarcopenia is almost 10% at age 85. Probable osteosarcopenia significantly increased risk of hip and major osteoporotic fractures and mortality more so than low bone mass alone.
Sensing chemical-induced DNA damage using CRISPR/Cas9-mediated gene-deletion yeast-reporter strains
Authors: Yamamoto Kosuke , Tochikawa Shintaro , Miura Yuuki , Matsunobu Shogo , Hirose Yuu , Eki Toshihiko 
Published: February 2024

Abstract
Microorganism-based genotoxicity assessments are vital for evaluating potential chemical-induced DNA damage. In this study, we developed both chromosomally integrated and single-copy plasmid–based reporter assays in budding yeast using a RNR3 promoter–driven luciferase gene. These assays were designed to compare the response to genotoxic chemicals with a pre-established multicopy plasmid–based assay. Despite exhibiting the lowest luciferase activity, the chromosomally integrated reporter assay showed the highest fold induction (i.e., the ratio of luciferase activity in the presence and absence of the chemical) compared with the established plasmid-based assay. Using CRISPR/Cas9 technology, we generated mutants with single- or double-gene deletions, affecting major DNA repair pathways or cell permeability. This enabled us to evaluate reporter gene responses to genotoxicants in a single-copy plasmid–based assay. Elevated background activities were observed in several mutants, such as mag1Δ cells, even without exposure to chemicals. However, substantial luciferase induction was detected in single-deletion mutants following exposure to specific chemicals, including mag1Δ, mms2Δ, and rad59Δ cells treated with methyl methanesulfonate; rad59Δ cells exposed to camptothecin; and mms2Δ and rad10Δ cells treated with mitomycin C (MMC) and cisplatin (CDDP). Notably, mms2Δ/rad10Δ cells treated with MMC or CDDP exhibited significantly enhanced luciferase induction compared with the parent single-deletion mutants, suggesting that postreplication and for nucleotide excision repair processes predominantly contribute to repairing DNA crosslinks. Overall, our findings demonstrate the utility of yeast-based reporter assays employing strains with multiple-deletion mutations in DNA repair genes. These assays serve as valuable tools for investigating DNA repair mechanisms and assessing chemical-induced DNA damage.

Key points
• Responses to genotoxic chemicals were investigated in three types of reporter yeast.
• Yeast strains with single- and double-deletions of DNA repair genes were tested.
• Two DNA repair pathways predominantly contributed to DNA crosslink repair in yeast.
Electrochemical and optical properties of poly(4-(4-(1H-pyrrol-1-yl)phenyl)-4H-dithieno[3,2-b:2',3'-d]pyrrole
Authors: Bezgin Carbas Buket , Ergin Nurseli Mislina , Yildiz Huseyin Bekir , Kivrak Arif 
Published: February 2024

AbstractA novel dithieno[3,2-b:2',3'-d]pyrrole (DTP) derivative, namely, 4-(4-(1H-pyrrol-1-yl)phenyl)-4H-dithieno[3,2-b:2',3'-d]pyrrole (DTP-Ph-Pyr) was synthesized, and its corresponding polymer (P(DTP-Ph-Pyr)) was successfully obtained via electrochemical polymerization. Electrochemical and optical properties of this novel polymer were discussed in detail. It was found that the polymer film also exhibited a reversible electrochromic behavior (orange color in the neutral state and blue color in the oxidized state) with a high optical contrast (52.5% at 950 nm) and coloration efficiency (123 cm2/C at 950 nm). Moreover, the electrochemical and optical properties of the polymer were compared with both its electrochromic P(DTP) analogues and its sister polymer structure, poly(1-(4-(1H-pyrrol-1-yl)phenyl)-2,5-di(thiophen-2-yl)-1H-pyrrole) (P(SNS-Ph-Pyr)), possessing same subunit.
Graphical abstract
Neurodevelopmental evaluation of children who were operated due to congenital anomaly with the ‘Ages and Stages Questionnaire (ASQ)’ and ‘ASQ: Social–Emotional’
Authors: Turedi Bilge , Gollu Gulnur , Ates Ufuk , Kose Kenan , Oner Ozgur , Bingol-Kologlu Meltem , Yagmurlu Aydin , Aktug Tanju , Dindar Huseyin , Cakmak Murat 
Published: February 2024

Abstract
Background
The purpose of surgeries performed for congenital anomalies in children is to increase the survival rates and provide a developmental comparison to that of their peers.

Aim
The objective of this study was to investigate the development of children following surgery for congenital anomalies and the risk factors affecting their development.

Methods
Our study included 33 children who underwent surgery for gastrointestinal anomalies in our clinic between 2011 and 2016, and did not have any syndrome, chromosomal abnormality, or additional abnormality. Developmental levels were evaluated using the Ages and Stages Questionnaire (ASQ) and the ASQ: Social–Emotional (ASQ: SE) scales adapted for the use on Turkish children. Data on patient history were obtained retrospectively from patient files.

Results
The study included 33 patients, including 11 with esophageal atresia, 6 with intestinal atresia, 11 with anorectal malformation, and 5 with Hirschsprung's disease. Developmental delay was found in the ASQ of 72.7% of the patients and the ASQ: SE tool was 27% of the patients. The rate of patients with scores below the threshold from each parameter of ASQ was higher than that of the normal population (p < 0.05). Development delay was detected using the ASQ scale in 100% of those with microcephaly at birth, in 91% of premature infants born between 1500 and 2500 g, and in 83.3% of those with low birth weight to gestational age.

Conclusions
In children who underwent surgery due to congenital anomalies, an evaluation through developmental tests, a post-surgical follow-up process, and a referral to the relevant disciplines when necessary may increase the success of surgery as well as increase the life quality of the patient.
Validating the use of clinical MSCT scans for cranial nonmetric sex estimation in a contemporary Indonesian population
Authors: Lye Ridhwan , Obertová Zuzana , Bachtiar Nur Amelia , Franklin Daniel 
Published: February 2024

AbstractThere is renewed interest in Asia for the development of forensic anthropological standards, partly due to the need for population-specific models to maintain high classification accuracies. At present, there are no known studies utilising morphoscopic standards specific to the Indonesian population. Craniometric analyses can often be time-consuming; morphoscopic assessments are quicker and are also known to be reliable and accurate. One of the most utilised morphoscopic standards for the estimation of skeletal sex is that of Walker (2008). Its application across population groups demonstrated reduced accuracies outside of the United States; population-specific predictive models would thus serve to improve the identification process of unknown skeletal remains. Digital imaging also allows for the validation of standards on a contemporary population and is an appropriate proxy to physical skeletal material.The present study quantifies the applicability of the Walker standard to a contemporary Indonesian population. A total of 200 cranial MSCT scans from a hospital in Makassar were analysed using OsiriX®. Scoring was performed in accordance with the Walker standard. Five univariate and nine multivariate predictive models were derived using single trait and multi-trait combinations. The best performing univariate model included the glabella, with a total classification accuracy of 82.0% and a sex bias of 14.6%. Classification accuracy with all traits considered was at 95.2% for females and 82.8% for males with a sex bias of 12.5%. These results provide forensic practitioners in Indonesia with an appropriate morphoscopic sex estimation standard, strengthening their capabilities in the field and improving judicial outcomes.
Association of triglyceride‑glucose index in branch retinal vein occlusion
Authors: Aslan Sirakaya Hatice , Sirakaya Ender 
Published: February 2024

Abstract
Background
To investigate the association between the triglyceride-glucose (TyG) index and newly diagnosed branch retinal vein occlusion (BRVO) in patients.

Methods
The study included 57 individuals with BRVO and a control group comprising 50 healthy volunteers matched for age and gender. Detailed eye examinations were conducted, and various blood biochemistry and hematological parameters were recorded. The TyG index was calculated using fasting plasma glucose and triglyceride values.

Results
The mean age was 61.4 ± 9.6 years for the BRVO group and 60.6 ± 10.3 years for the control group. The TyG values were significantly higher in the BRVO group when compared to the control group (8.84 ± 0.41 vs. 8.52 ± 0.29, p < 0.001). Multivariate analysis revealed that the TyG index independently predicted BRVO (odds ratio = 2.58, 95% confidence interval = 1.69–3.93; p < 0.001). In receiver operating characteristics analysis, the TyG index had an area under the curve of 0.749, and a TyG index higher than 8.52 predicted BRVO with 83% sensitivity and 70% specificity.

Conclusions
This study establishes a significant association between an elevated TyG index and BRVO. Consequently, the TyG index could serve as a valuable predictive tool for identifying individuals at risk for BRVO.
Short term e-bicycle riding results in favorable cardiometabolic shifts in moderately active adults
Authors: Alessio Helaine M. , Ballard Kevin D. , Reidy Paul T. , Hayward Katie M. , Bagg Alexandra M. , Cooley Rachel A. , O’Connell Michael J. , Montoye Alexander H. K. , Timmerman Kyle L. 
Published: February 2024

Abstract
Purpose
Electric bikes (EB) are a form of active transportation with demonstrated health benefits. The purpose of this study was to determine the influence of riding an EB for one week on indices of cardiometabolic health in middle-aged adults.

Methods
Adults (n = 22; age = 57.1 ± 11.3 year; BMI = 27.7 ± 4.9) participated in a 2 week study. During Week 1, participants were instructed to continue regular activities. Starting Week 2 participants were provided an EB to ride at least 3 days for a minimum of 30 min·day−1. Physical activity (PA) and glucose were measured continuously. Body composition, blood lipids, glucose, insulin, hemoglobin A1c (HbA1c), plasma endothelin-1 (ET-1), and carotid-femoral pulse wave velocity (cf-PWV) were measured on days 1 and 14.Data and Statistical analyses or Statistics. Each participant served as their own control. Paired t-tests compared dependent variables between week 1 (without EB) and week 2 (with EB).

Results
When provided an EB for one week, moderate to vigorous PA increased by 6–9 min·day−1 (P < 0.05) and sedentary time decreased by ~ 77 min·day−1 (P < 0.05). Data from 24 h continuous glucose monitoring showed the percentage of time in healthy range (70–120 mg·dl−1 glucose) increased (P < 0.05) from week 1 to week 2. Compared to day 1, cf-PWV was lower at day 14 (P < 0.05) following one week of riding an EB.

Conclusion
Moderately-active, middleaged adults showed improved continuous glucose regulation and lower central arterial stiffness following one week of riding an EB.
Is the European badger a new host for Dirofilaria immitis? The first records in Greece
Authors: Markakis Grigorios , Sioutas Georgios , Bitchava Dimitra , Komnenou Anastasia , Ganoti Maria , Papadopoulos Elias 
Published: February 2024

AbstractDirofilaria immitis is a ubiquitous nematode parasite with zoonotic potential, transmitted by mosquitoes, that causes heartworm disease in various animal species. Dogs are the parasite’s typical final host, and wild carnivores represent the parasite’s reservoir in nature. Studies on D. immitis infections in wild animals are essential to assess infection pressure for domestic animals, and until now, there has been only one infection case reported in a European badger (Meles meles). The current report describes the first two European badger cases with cardiovascular dirofilariosis in Greece. Two adult male badgers were rescued in Heraklion and Chania, Crete Island, and admitted to “ANIMA -Wildlife Rehabilitation Centre” in Athens. The detailed clinical examination revealed that the first badger suffered from severe broncho-pneumonitis while the second one displayed clinical signs associated with severe brain trauma. Blood samples were taken for haematology and biochemistry analyses during their short hospitalisation period. In addition, different routine diagnostic tests were carried out, including heartworm antigen testing (ELISA) and the modified Knott’s test for microfilariae. Both badgers were positive in both tests. The animals died a few hours after their admission and the detailed necropsies followed, revealed the presence of three parasites in each animal’s right heart, morphologically identified as adults of D. immitis. These findings add the European badger in the list of additional potential reservoir hosts for D. immitis and highlight the potential role of wildlife for companion animals and human health.
Whole exome sequencing and polygenic assessment of a Swedish cohort with severe developmental language disorder
Authors: Yahia Ashraf , Li Danyang , Lejerkrans Sanna , Rajagopalan Shyam , Kalnak Nelli , Tammimies Kristiina 
Published: February 2024

AbstractDevelopmental language disorder (DLD) overlaps clinically, genetically, and pathologically with other neurodevelopmental disorders (NDD), corroborating the concept of the NDD continuum. There is a lack of studies to understand the whole genetic spectrum in individuals with DLD. Previously, we recruited 61 probands with severe DLD from 59 families and examined 59 of them and their families using microarray genotyping with a 6.8% diagnostic yield. Herein, we investigated 53 of those probands using whole exome sequencing (WES). Additionally, we used polygenic risk scores (PRS) to understand the within family enrichment of neurodevelopmental difficulties and examine the associations between the results of language-related tests in the probands and language-related PRS. We identified clinically significant variants in four probands, resulting in a 7.5% (4/53) molecular diagnostic yield. Those variants were in PAK2, MED13, PLCB4, and TNRC6B. We also prioritized additional variants for future studies for their role in DLD, including high-impact variants in PARD3 and DIP2C. PRS did not explain the aggregation of neurodevelopmental difficulties in these families. We did not detect significant associations between the language-related tests and language-related PRS. Our results support using WES as the first-tier genetic test for DLD as it can identify monogenic DLD forms. Large-scale sequencing studies for DLD are needed to identify new genes and investigate the polygenic contribution to the condition.
Amino acid distribution in blood following high-intensity interval exercise: a preliminary study
Authors: Borges Nattai , Doering Thomas M. , Murphy Grace , Macdonald Margaret , Dunstan Richard H. 
Published: February 2024

AbstractThis study investigated the effect of high-intensity interval exercise on total and individual amino acid concentrations in red blood cells (RBCs) and plasma. Seven males (31 ± 13 yr) provided venous blood samples at rest, immediately and 15 min and 30 min following an 8-min high-intensity exercise bout. The exercise bout was 16 × 15 s cycle efforts at 0.4N/kg of body mass and 90 rpm, interspersed with 15 s passive recovery. Total and individual amino acid concentrations of RBC and plasma and blood cell parameters were analysed. No significant differences for total amino acid concentrations between RBC and plasma were found. Individual amino acid analyses showed significant interaction effects for alanine and α-aminoadipic acid (P < 0.05), with plasma alanine significantly increased from baseline across the recovery period (P < 0.001). Blood fraction (group) effects showed greater concentrations of glycine, serine, asparagine, aspartic acid, glutamic acid, α-aminoadipic acid and ornithine in RBC, while greater concentrations of alanine, α-aminobutyric acid, valine, leucine, isoleucine, threonine, proline, phenylalanine, glutamine, tryptophan and cystine were found in plasma (P < 0.05). Comparable levels of histidine, lysine and tyrosine were observed between blood fractions. Significant differences in the variation of total amino acids in RBC were reported with higher variance at rest compared to following exercise (P = 0.01). Haemoglobin, pack cell volume and white blood cell count significantly increased immediately following exercise (P < 0.05) but returned to baseline after 15 min recovery. These results support the notion of individualised amino acid transportation roles for RBC and plasma during exercise.
Peer relationships, adolescent anxiety, and life satisfaction: a moderated mediation model in Turkish and syrian samples
Authors: Yetim Onat , Çakır Resul , Bülbül Ece , Alleil İlham Sebea 
Published: February 2024

AbstractPrior studies comparing Syrian refugee adolescents to their native peers in the same region have found higher anxiety and lower life satisfaction. Therefore, identifying regulatory variables is crucial for implementing support programs. This study examined the mediating effect of peer relationships and the moderating effect of being a refugee or native adolescent on the relationship between adolescent anxiety and life satisfaction across different samples. Participants and setting: The study included 2,336 adolescents aged 11–19 (M = 14.79, SD = 1.04). Participants completed the Screen for Child Anxiety Related Disorders, Satisfaction with Life Scale, and Strengths and Difficulties Questionnaire. The mediation and moderation effects were analyzed with the path analysis codes written on Mplus 8.3. SPSS 26 was used for descriptive statistics and group comparisons. The findings showed that peer relationships mediate adolescent anxiety and life satisfaction, and this relationship is moderated according to whether the participants are native adolescents or refugee adolescents. This study highlights the significant associations between peer relationships, adolescent anxiety, and life satisfaction and the moderating role of the participant identity. The findings may inform psychological interventions to improve Syrian refugee adolescents' mental health and well-being. These findings may also have implications for policies and programs aimed at supporting the integration of Syrian refugee adolescents in host communities.