bluuebunny/crossref_metadata_2025_split huggingface dataset:
DAHEAN: A Danish Nationwide Study Ensuring Quality Assurance through Real-World Data for Suspected Hereditary Anemia Patients
Authors: Glenthøj Andreas , Rasmussen Andreas Ørslev , Bendtsen Selma Kofoed , Hasle Henrik , Hoffmann Marianne , Rieneck Klaus , Dziegiel Morten Hanefeld , Sjö Lene Dissing , Frederiksen Henrik , Hansen Dennis Lund , Fassi Daniel El , Rathe Mathias , Jensen Peter-Diedrich Matthias , Larsen Anne Winther , Nielsen Christian , Olsen Marianne , Toft Nina , Lorenzen Mads Okkels Birk , Jensen Lise Heilmann , Gudbrandsdottir Sif , Helby Jens , Rossing Maria , Wijk Richard van , Petersen Jesper 
Published: February 2024

Abstract

Background
Hereditary anemias is a group of genetic diseases prevalent worldwide and pose a significant health burden on patients and societies. The clinical phenotype of hereditary anemias varies from compensated hemolysis to life-threatening anemia. They can be roughly categorized into three broad categories: hemoglobinopathies, membranopathies, and enzymopathies. Traditional therapeutic approaches like blood transfusions, iron chelation, and splenectomy are witnessing a paradigm shift with the advent of targeted treatments. However, access to these treatments remains limited due to lacking or imprecise diagnoses.
Results
The DAHEAN study is a nationwide cohort study that collects advanced phenotypic and genotypic data from patients suspected of having hereditary anemias from all pediatric and hematological departments in Denmark. The study deliberates monthly by a multidisciplinary anemia board involving experts from across Denmark. The primary objective of the study is to establish accurate diagnoses for patients with hereditary anemias, enabling optimal management. As a secondary objective, the study aims to enhance our diagnostic capabilities. So far, fifty-seven patients have been thoroughly evaluated, and several have been given diagnoses not before seen in Denmark.
Conclusions
The DAHEAN study and infrastructure harness recent advancements in diagnostic tools to offer precise diagnoses and improved management strategies for patients with hereditary anemias.
Association of Dietary Acid Load and Depression among Iranian Women: a case-control study
Authors: Hosseinzadeh Mahdieh , Darand Mina , Ghorbani Moloud , Arabi Vahid 
Published: February 2024

Abstract

Background The present study aimed to assess the association between Dietary Acid Load and Depression in Iranian women.Material and methods This case-control study was conducted among 110 depressed women and 220 control subjects from two psychiatric clinics in Tehran, Iran. A validated semi-quantitative food frequency questionnaire consisting of 168 items was used to assess food consumption in the previous 12 months. The dietary acid load was calculated using the relevant data with 2 validated methods: PRAL and NEAP. The association between depression and PRAL and NEAP was investigated using multivariate logistic regression in crude and adjusted models.Results The findings of the present study showed participants in the highest quartile of PRAL and NEAP had a 4.11- and 4.43-times higher risk of depression than the participants in the lowest quartile, respectively (Odds Ratio (OR)PRAL: 3.52; 95% (CI):1.64 to 7.54, p for trend: <0.001) and (Odds Ratio (OR)NEAP: 4.07; 95% (CI):1.81 to 8.89, p for trend: <0.001).Conclusion there is a significant inverse association between dietary acid load scores (PRAL and NEAP) and the odds of depression in women. However more studies are needed to support these findings in the future.
Assessment of the Validity of the Resilience and Strain Questionnaire in Caregivers of People with Dementia (ResQ-Care-Dem)
Authors: Blotenberg Iris , Wuttke Alexandra , Boekholt Melanie , Köhler Kerstin , Holle Bernhard , Thyrian Jochen René 
Published: February 2024

Abstract

Background
To support informal caregivers of people with dementia, it is crucial to identify sources of stress and burden, recognize early warning signs for timely intervention, and actively promote resilience. The Resilience and Strain Questionnaire in Caregivers of People with Dementia (ResQ-Care-Dem) has been developed to identify particularly vulnerable caregivers as early as possible and to derive specific recommendations for action. This article examines its reliability and construct validity.
Methods
The ResQ-Care-Dem was completed online by n = 243 informal caregivers of people with dementia. The reliability of the two resilience and two burden scales was assessed using Cronbach's alpha as a measure of internal consistency. The factorial structure was examined using a principal axis factor analysis. The convergent validity of the burden scales was assessed by correlations with the Zarit Burden Interview (ZBI-7). The convergent validity of the resilience scales was assessed using the Caregiver Self-Efficacy Scale (CES-8), the Gain in Alzheimer Care Instrument (GAIN), and the Quality of the Carer-Patient Relationship (QCPR) Questionnaire.
Results
The scales’ internal consistencies ranged between .65 and .81. The factorial structure could largely be confirmed, with the items of the four scales primarily loading on four factors. The burden scales demonstrated the expected high correlations with the ZBI-7 (.51 – .55). As expected, the resilience scales showed moderate to strong correlations with the CES-8 (.50 – .57), the GAIN (.27 – .50), and the QCPR (.28 – .59).
Conclusions
Overall, this study provided evidence supporting the reliability and construct validity of the ResQ-Care-Dem. In particular, the high correlations with other measures of the caregiver's situation suggest that the questionnaire is a concise yet comprehensive instrument for measuring stress and burden, as well as resilience factors in informal dementia caregivers simultaneously.
Development and validation of a risk assessment model for seizure recurrence during anti-seizure medications withdrawal
Authors: zhang yanyan , huang wenwen , zhu shuxia , zhang yuehua , sun jinkai 
Published: February 2024

Abstract

This study is mainly to develop a risk assessment model for seizure recurrence during anti-seizure medications(ASMs) withdrawal in children and to evaluate and validate the model. Retrospective collection of children with epilepsy from January 2018 to December 2020 in the Department of Pediatrics Neurology in Binzhou Medical Universtiy Hospital who were in the process of ASMs withdrawal,They included 142 participants as the training group. The training group was divided into a relapse group and a non-relapse group according to whether the seizures had recurred,Clinical data of the training group were collected and univariate analysis was performed, then multivariate logistic regression was performed on the selected data,The prediction model was presented in the form of nomogram to evaluate the differentiation and calibration degree of the prediction model.Internal validation was also performed. The results showed that the seizure recurrence rates in the training group and the validation group were respectively 28.46% and 25%.By multivariate logistic regression, we found that seizure types, long-term freedom before ASMs withdrawal, abnormal electroencephalogram (EEG) before ASMs withdrawal and polypharmacy were independent risk factors.It is showed in the form of a formula and a nomogram.The model has good discrimination and calibration.So we conclude that seizure types, long-term seizure freedom before ASMs withdrawal, abnormal EEG before ASMs withdrawal and polypharmacy are independent risk factors.Based on these four risk factors to build a prediction model can accurately predict the risk probability of seizure recurrence, in order to reduce the ASMs in the early detection of seizure recurrence risk, advance intervention measures.
Monitoring and assessment of heavy metal transfer from soil to Beta vulgaris L. (sugar beet) in Kayseri, Turkey
Authors: BÜLBÜL Hayrun Nisa , LEBLEBİCİ Zeliha 
Published: February 2024

Abstract
In our study, sugar beet plants grown in Yeşilhisar district of Kayseri were used. 6 different locations were selected: Kesik, Dağılgan, Hacılımezarlığı, Kıraç Tepe, Hacıbektaş and the control area (Kilcan). Leaf, beet, root and soil samples were taken from Beta vulgaris plant. Heavy metal (As, Cd, Pb, Cu, Ni and Cr) contents were analyzed using ICP-MS device. In terms of sugar beet consumption in Kayseri province, Turkey, determining heavy metal accumulation and health risks will be very important in terms of these metals. Heavy metal concentration levels in sugar beet samples collected from the region are as follows; nickel (Ni) > copper (Cu) > chromium (Cr) > arsenic (As) > lead (Pb) > cadmium (Cd). When As, Cd and Cr elements are compared with the limit values, it is observed that they are within the allowed limits. Cu, Ni and Pb indicate the potential for negative contributions to health through sugar beet consumption. The study reveals health concerns regarding the heavy metal content of sugar beet samples grown in Yeşilhisar, Kayseri province and recommends that necessary measures be taken to reduce the occurrence of Cu, Ni and Pb observed in sugar beet samples from this region.
Including Harvested Grain Related CO2 in Climate Accounting
Authors: Gray Richard Stanley 
Published: February 2024

Abstract

The current international climate greenhouse gas accounting system excludes the CO2 removed from the atmosphere and stored in harvested crops, and the CO2 emissions that occur when crops are oxidized, viewing both fluxes as carbon-neutral activities. Despite being previously described by Searchinger et al. (2009) as a “critical flaw in climate accounting,” all parties within the United Nations Framework Convention on Climate Change continue to treat these CO2 fluxes as carbon-neutral activities. A two-region trade model is used to show that when harvested-grain biomass as carbon-neutral there is an incentive for parties committed to reducing emissions under the Paris Agreement to develop policies that use grains to produce biofuels, with no offsetting policy incentive to produce the grains required to meet this additional demand. A solution to the flaw in accounting is to treat harvested grain CO2 fluxes the same as fossil fuel CO2 and expand National GHG Inventory accounts to include the CO2 sequestered in harvested grains, and the CO2 emissions that occur when grain is consumed. Including these two additional lines in National GHG Inventories would more accurately measure national emissions and would incentivize parties in the Paris agreement to increase net grain exports, thereby enhancing food security while reducing the market incentives for carbon intensive land use conversion in non-compliant countries. Applying this more comprehensive Net Ecosystem Exchange (NEE) accounting system to Canadian agricultural emissions over the past decade, demonstrates the simplicity of accounting, the magnitude of impacts, and the profound impact on policy incentives.
Application of the needle-thread retained stitch in enterostomy
Authors: Lifeng Xie , Jing Jia , Xinyu Li 
Published: February 2024

Abstract

Purpose intestinal stoma may be performed using various techniques.Each technology has its own unique characteristics.In this report,we report the application of the needle-thread retained stitch in enterostomy.
Methods A prospective cohort study,126 patients who were divided into two groups randomly to perform enterostomy with the needle-thread retained stitch and the traditional intermittent stitch respectively.The enterostomy operation and preoperative general clinical data were recorded,and postoperative complications were observed.
Results enterostomys were performed successfully in 126 patients,68 patients performed the needle-thread retained stitch and 58 patients performed the traditional intermittent stitch).The median enterostomy operative time to create the stoma was 19 min(range,15～26 min)(the needle-thread retained stitch),22 min(range, 17～32 min)(the traditional intermittent stitch).The median blood loss was 1 ml(range,1～10 ml)(the needle-thread retained stitch),2 ml(range, 1～15 ml)(the traditional intermittent stitch).Complications related to stoma occurred in 3 cases (4.41%) in the former, while 5 cases (8.62%) in the latter.Univariate analysis show that the complications related to stoma were correlate with age，BMI, preoperative intestinal obstruction, preoperative leukocyte level, hemoglobin level, plasma albumin level, intestinal edema, postoperative dysuria and pulmonary infection (p＜0.05).Multivariate logistic regression analysis show that BMI, preoperative hemoglobin, intestinal edema, postoperative dysuria and pulmonary infection were independent risk factors for enterostomy related complications.
Conclusion The needle-thread retained stitch apply to enterostomy is feasible, safety and reliable, which reduces the incidence of intraoperative complication and postoperative ostomy related complication.It has the value of clinical application and popularization.
Volatile Compounds in Endophyte Infected Festuca arundinacea: Impact on Heteronychus Arator Host Selection
Authors: Qawasmeh Abdel , Raman Anantanaraynan , Wheatley Warwixk 
Published: February 2024

Abstract
Mediterranean (M) and Continental (C) Festuca arundinacea cultivars in Australia are either endophyte–free (E–) or uniquely infected with the fungal endophyte Neotyphodium coenophialum strain AR542 (EAR542). The impact of volatile oils produced by Mediterranean and Continental cultivars in response to EAR542 on enhancing the grass’s resistance to insects is still largely unknown. In this study, we determined the volatile-oil profiles produced by ME–, CE–, MEAR542 and CEAR542 cultivars and their impacts on the host-selection behavior of H. arator adults. A total of 22 volatile compounds were detected in all F. arundinacea samples tested irrespective of being from Mediterranean or Continental cultivars and with or without endophyte. Octanal, 2-ethylhexanol, dodecane, (Z)-2-hexen1-1-ol, butyl hexanoate, (Z)-3-hexen-1-ol, 1-octen-3-ol, and butylated hydroxytoluene were the major compounds identified and variably influenced by the germplasm and endophyte infection status. Quantitatively, ME– expressed lesser quantities of 1,6-octandien-1-ol, hexanal, hexanol, 1-octen-3-ol and ethyl-3-hydroxypentanoate compared with CE– but greater quantities of butylated hydroxytoluene and hexylhexanoate. Compared with ME–, the quantities of octanal, 2-ethylhexanol, pentanal, hexanal, undecanal, 1-octen-3-ol, (Z)-2-octen-1-ol and clovene were greater in MEAR542. However, compared with CE–, hexanal, undecanal, undecane, i-octen-3-ol, (Z)-2-octen-1-ol, cloven and butylated hydroxytoluene were greater in CEAR542. In four-choice assay, the percentage of the numbers of H arator adult males choosing one of the choices ME–, MEAR542, CE– or CEAR542 was not significantly different compared to females choosing any of the respective choices. The impact of the germplasm on the host selection of H. arator adults was determined using two-choice assay olfactometer. The H. arator adults significantly (χ2 = 10 d.f. = 1, P < 0.002) preferred ME– compared with CE–. In contrast, the CEAR542 was significantly (χ2 = 26.67, d.f. = 1, P < 0.05) preferred host by same beetles compared with MEAR542. Mediterranean germplasm is different to the Continental germplasm in relation to the quantities of volatile compounds produced and these quantities in Mediterranean and Continental cultivars variably influenced by EAR542 infection. Whether changes in quantities of the volatile compounds in EAR542–infected grasses impose any agricultural or ecological impact require further investigation.
Impact of interstitial lung disease Gender-Age-Physiology index in surgically treated lung cancer
Authors: Sakamoto Shinichi , Kawakita Naoya , Takeuchi Taihei , Sumitomo Hiroyuki , Miyamoto Naoki , Toba Hiroaki , Kondo Kazuya , Takizawa Hiromitsu 
Published: February 2024

Abstract

Background The postoperative prognosis of patients with interstitial lung disease (ILD) and lung cancer is poor. Recently, the ILD-Gender-Age-Physiology (GAP) index was identified as a clinical prognostic factor for patients with ILD. This study investigated the ILD-GAP index and oncological factors regarding postoperative outcomes.Methods We retrospectively reviewed 87 lung cancer patients with comorbid ILD who underwent curative resection at our institution between April 2005 and December 2019. Short-term postoperative outcomes and overall survival (OS) based on the ILD-GAP index were examined. OS rates after surgery were calculated using the Kaplan-Meier method, and group differences were analyzed using the log-rank test. Univariate and multivariate analyses for OS were performed using the Cox regression model.Results Multivariate analyses revealed ILD-GAP index ≥ 4 [Hazard ratio, 3.349; 95% confidence interval, 1.375–8.155; P = 0.008] as a factor associated with OS. In the ILD-GAP index ≥ 4 group, no deaths occurred from primary lung cancer, with respiratory-related deaths being the most common, and exacerbation of ILD was more frequent (P = 0.007). Regarding perioperative results, a significant difference was observed in 90-day mortality (2.7% vs 23.0% [P = 0.022]), and more patients required Home Oxygen Therapy (14.9% vs 69.2% [P༜0.001]) in the ILD-GAP index ≥ 4 group.Conclusions An ILD-GAP index ≥ 4 indicated a poor prognostic factor for patients with surgically treated lung cancer. Careful consideration of surgical indications is essential for patients with an ILD-GAP index ≥ 4.
CRISPR-Cas13a triggered DNAzyme signal amplification-based colorimetric miRNA detection method and its application in evaluating the anxiety
Authors: Yan Na , Hu Zhiwei , Zhang Lingling 
Published: February 2024

Abstract

The development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof-of-concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe. As a consequence, the S chain was liberated from the T chain that had been modified on magnetic beads (MB). The G-rich sections were then exposed when the catalytic hairpin assembly between the H1 and H2 probes was activated by the released T@MB. G-rich section can fold into G-quadruplex. By catalyzing the formation of green ABTS³– via HRP-mimicking G-quadruplex/hemin complexes, colorimetric measurements of miRNA can be achieved visually through DNAzyme-mediated signal amplification. The method demonstrated a low limit of detection of 27 fM and a high selectivity towards target miRNA eventually. As a result, the developed strategy provides a clinical application platform for the detection of miRNAs that is both ultrasensitive and extremely specific.
Out of Hospital Cardiac Arrest - New Insights and a Call for a Worldwide Registry and Guidelines
Authors: Tabi Meir , Perel Nimrod , Taha Louay , Amsalem Itshak , Hitter Rafi , Maller Tomer , Manassra Mohamed , Karmi Mohammad , Zacks Netanel , Levy Nir , Shrem Maayan , Marmor David , Gavriel David , Jarjoui Amir , Shuvy Mony , Asher Elad 
Published: February 2024

Abstract

Introduction:
Out of hospital cardiac arrest (OHCA) is a common, catastrophic event. Genetic diseases and primary electrical disorders are the most common etiologies at younger ages, while ischemic heart disease and cardiomyopathies are common causes at older ages. Despite improvement in prevention and treatment in recent years, OHCA is still a major cause of cardiovascular death.
Method
We report prospective data regarding etiology, characteristics, clinical course, and outcomes of patients with OHCA who were admitted to a tertiary care center intensive cardiac care unit (ICCU) between 2020–2023.
Results
A total of 92 patients admitted after OHCA were included in the cohort. Mean age was 63.8 ± 13.8 years old and 75 (82%) were male. The most common etiology of OHCA was acute coronary syndrome (ACS) in 54 (59%) patients, of whom 46 (85%) patients had ST elevation myocardial infarction and 8 (15%) had non-ST elevation myocardial infarction. During hospitalization, 42 (46%) patients underwent targeted temperature management and 13 (14%) received mechanical circulatory support. Interestingly, 77 (84%) patients underwent coronary angiography, while only 51 (55%) received percutaneous coronary intervention (PCI). Neurologic status was favorable in 49 (53%) patients with Cerebral Performance Category score of 1–2. Overall, mortality rates were relatively low, with 15 (16%) in-hospital deaths and 24 (26%) deaths at 30-day follow-up.
Conclusion
Although ACS was the most common etiology for OHCA, only 55% of patients underwent PCI. Most OHCA patients admitted to the ICCU survived hospitalization and were discharged. Increased awareness, public education, worldwide registries, and specific guidelines for the treatment of OHCA patients may lead to improved outcomes for these patients who often carry poor prognoses. A worldwide database may help to better understand and treat these patients.
Gastro-gastric intussusception after Roux-en-Y gastric bypass: case report and literature review of an unusual complication.
Authors: Restrepo Hernán Restrepo , Uribe Gabriel Arroyave , Moreno Santiago Barrantes , González Camila Arias , Franco Sara Patiño 
Published: February 2024

Abstract
Introduction: Intestinal intussusception is a rare pathology that usually involves the small intestine and almost always has an identifiable cause in adult patients. It may occur as a rare complication after Roux-en-Y gastric bypass. In recent years, due to the sustained increase of obesity in developed and developing countries, there has been an increase in the use of bariatric surgery as a treatment for this pathology, and among the most commonly performed is the Roux-en-Y gastric bypass. Cases of intussusception have been described in this context, almost always involving jejuno-jejunal anastomosis.
Case presentation: In this article we present a patient who, after Roux-en-Y gastric bypass, developed a gastrogastric intussusception, which was corrected with resection of the gastric remnant in a satisfactory manner.
Conclusions: Intussusception after Roux-en-Y gastric bypass is a rare complication. It is necessary to have a high degree of suspicion in patients with gastrointestinal symptoms with a history of gastric bypass, and in this cases resection of the affected segment, and not just reduction with or without fixation, is the optical management.
Pachyneuron (Hymenoptera: Pteromalidae) and its relationship to ladybirds (Coleoptera: Coccinellidae): new data from Iran and review of the literature
Authors: Biranvand Amir , Ceryngier Piotr , Vahedi Hassanali , Romasi Fatemeh , Ghobari Hamed , Nedvěd Oldřich 
Published: February 2024

Abstract
Species of Pachyneuron Walker are most often reported as hyperparasitoids of aphids, coccids and psyllids (Hemiptera: Sternorrhyncha). On the other hand, quite many reports concern hyperparasitism in the predators of Sternorrhyncha, mainly ladybird beetles. In this paper we report on the association of Pachyneuron muscarum (L.) with a ladybird Hippodamia variegata (Goeze) in Kermanshah (Iran). One male of P. muscarum was bred from H. variegata larva parasitized by a primary parasitoid of the genus Homalotylus Mayr (Hymenoptera: Encyrtidae) and the other specimen (female), from a larva of the same ladybird species parasitized by a parasitoid other than Homalotylus. Previously, P. muscarum was reported from Iran in association with several species of coccids and psyllids, as well as predatory lacewing (Neuroptera: Chrysopidae) and ladybird larvae. A worldwide literature review indicates that at least eight species of Pachyneuron have been reported as hyperparasitoids of ladybird larvae. It may be thus concluded that some Pachyneuron species, being principally hyperparasitoids of Sternorrhyncha, can also hyperparasitize Sternorrhyncha-preying ladybird larvae.