bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful high‐titer immunoglobulin therapy for persistent parvovirus B19 infection in a lymphoma patient treated with rituximab‐combined chemotherapy
Authors: Isobe Yasushi , Sugimoto Koichi , Shiraki Yumi , Nishitani Miho , Koike Kengo , Oshimi Kazuo 
Published: November 2004

AbstractA 40‐year‐old female diagnosed with follicular lymphoma was treated with rituximab‐combined chemotherapy. Although she achieved complete remission, she developed progressive anemia and reticulocytopenia. Bone marrow examination revealed features of pure red cell aplasia and hemophagocytosis. In addition, the appearance of large pronormoblasts suggested that she was infected with parvovirus B19. Excess viral DNA in her bone marrow confirmed that her illness was caused by persistent parvovirus B19 infection. Serum immunoglobulin levels decreased beyond the lower normal limit, which indicated that her humoral immunity was impaired after rituximab‐combined chemotherapy. Although she had been infected with parvovirus B19, she was re‐infected and failed to control the viral expansion. High‐titer immunoglobulin against parvovirus B19 was intravenously administrated and resulted in remarkable reticulocytosis and improvement of anemia. High‐titer immunoglobulin, which contained a sufficient amount of neutralizing antibodies against parvovirus B19, likely inactivated most viruses in vivo. We successfully eradicated the virus after 2 courses of high‐dose therapy at 0.5 g/kg/day every week followed by 8 courses of maintenance therapy at 0.1 g/kg/day every other week. It is important to consider that parvovirus B19 infection is a possible cause of progressive anemia in B‐cell lymphoma patients treated with rituximab‐combined chemotherapy. We propose that the use of high‐titer immunoglobulin against parvovirus B19 may enable such immunocompromised patients to eradicate the virus before sufficient immune system reconstruction. Am. J. Hematol. 77:370–373, 2004. © 2004 Wiley‐Liss, Inc.
Factors affecting choice of hemostatic agent for the hemophilia patient with an inhibitor antibody
Authors: Monahan Paul E. , Aledort Louis M. 
Published: November 2004

AbstractAchieving adequate hemostasis in patients with hemophilia and high‐titer inhibitor antibodies remains a challenge despite the development of plasma‐derived and recombinant therapies to bypass the inhibitory effect. A group of hemophilia treaters met to examine the factors that influence physician choices among the very costly and cumbersome available therapies. Specific knowledge deficits in need of prospective research were identified with respect to the rational treatment of inhibitor patients. Am. J. Hematol. 77:346–350, 2004. © 2004 Wiley‐Liss, Inc.
Nonmyeloablative allogeneic bone marrow transplantation for treatment of myelodysplastic syndrome complicated by recent intracerebral hemorrhage
Authors: Ohtsubo Korenori , Takase Ken , Nagafuji Koji , Henzan Hideho , Tanimoto Tetsuya , Fukuda Takahiro , Miyamoto Toshihiro , Inaba Shoichi , Harada Mine 
Published: November 2004

AbstractA patient with intracerebral hemorrhage is considered ineligible for hematopoietic stem cell transplantation (HSCT). We report a 49‐year‐old woman with myelodysplastic syndrome (MDS) complicated by refractoriness to platelet transfusion and intracerebral hemorrhage, who underwent allogeneic bone marrow transplantation from an HLA‐identical unrelated male donor. Nine days before the scheduled transplantation, she developed dysarthria and right hemiparesis; computed tomography (CT) of the brain disclosed an acute hematoma in the left parietal lobe exceeding 3 cm in diameter. She underwent conditioning with reduced‐intensity, including fludarabine (30 mg/m2/day on days −8 to −3), busulfan (4 mg/kg/day on days −6 and −5), and total body irradiation (4 Gy on day −2). Two weeks after transplantation, dysarthria and right hemiparesis began to resolve, and CT showed spontaneous resolution of the hematoma. Simultaneously, engraftment was confirmed. Thus, recent stroke may be not an absolute contraindication for HSCT. Am. J. Hematol. 77:400–404, 2004. © 2004 Wiley‐Liss, Inc.
Poly(allyl glycidyl ether)‐block‐poly(ethylene oxide): A novel promising polymeric intermediate for the preparation of micellar drug delivery systems
Authors: Hrubý M. , Koňák Č. , Ulbrich K. 
Published: November 2004

AbstractNovel diblock copolymers designed for the preparation of micellar drug delivery systems, consisting of hydrophobic poly(allyl glycidyl ether) (PAGE) and hydrophilic poly(ethylene oxide) (PEO), were prepared, and their self‐assembly into micellar structures was studied. Copolymers differing in the length of the polymer blocks were purified and characterized. These amphiphilic copolymers with narrow molecular weight distributions were prepared through the anionic polymerization of allyl glycidyl ether with PEO monomethyl ether sodium salt as the macroinitiator. The PAGE–PEO copolymer readily formed small micelles with narrow size distributions via simple dissolution in water. The addition of pendant double bonds to the hydrophobic part of the chain was intended for further covalent modifications. Catalytic hydrogenation, the radical crosslinking of the micelle core, and the addition of thiol to double bonds of the copolymer were examples of such modifications that were proved to proceed with a quantitative yield for this copolymer. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 201–211, 2005
Effect of low‐temperature annealing on dimensional stability of poly(ethylene 2,6‐naphthalene dicarboxylate) fibers melt‐spun at high speed
Authors: Kang Young Ah , Kim Kyoung Hou , Cho Hyun Hok , Kikutani Takeshi 
Published: November 2004

AbstractTo investigate structural factors, necessary to obtain a valuable industrial fiber possessing excellent thermomechanical properties, poly(ethylene 2,6‐naphthalene dicarboxylate) (PEN) fibers were produced by high‐speed melt‐spinning to a take‐up speed of 8 km/min, followed by low‐temperature annealing between the glass‐transition temperature (Tg) and exothermic cold crystallization temperature (Tc cold), where little transition of crystalline phase, as well as little thermal degradation, takes place. Their thermomechanical behavior, as well as structural variations, were investigated through differential scanning calorimetry, Rheovibron, thermomechanical analysis (TMA), and tensile testing. Two types of the α‐ and α′‐dispersions were observed at near Tg and at a temperature 50–60°C higher than Tg, respectively. The dispersions were affected by rearranged structures, which are generated by developing an inhomogeneous taut structure with rigidity of aromatic segment and aliphatic segment. The α‐dispersion seemed to reflect an inhomogeneous taut structure by the less nearly arranged segments. Consequently, at intermediate take‐up speeds between 2 and 6 km/min the inhomogeneous taut structure may be partially formed, but the homogeneously ordered structure may be enlarged as the take‐up speed and annealing temperature increased. Thermal shrinkage increased above the α‐dispersion temperature, which suggested that the onset point of dimensional change in PEN fibers was attributed to α‐dispersion. In the case of annealed fibers, the start of length change coincided with the respective annealing temperatures, which indicated that dimensional stability could be gained from restraining the inhomogeneous taut structure in the amorphous region without the transition of crystalline phase by annealing between Tg and Tc cold. Therefore, to obtain dimensional stability in PEN fibers, it is supposed that the inhomogeneous taut structure exhibited by the α‐dispersion should be controlled. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 212–218, 2005
Effect of diluent on poly(ethylene‐co‐vinyl alcohol) hollow‐fiber membrane formation via thermally induced phase separation
Authors: Shang Mengxian , Matsuyama Hideto , Teramoto Masaaki , Okuno Junpei , Lloyd Douglas R. , Kubota Noboru 
Published: November 2004

AbstractPoly(ethylene‐co‐vinyl alcohol) hollow‐fiber membranes with a 44 mol % ethylene content were prepared by thermally induced phase separation. A mixture of 1,3‐propanediol and glycerol was used as the diluent. The effects of the ratio of 1,3‐propanediol to glycerol in the diluent mixture on the phase diagram, membrane structure, and membrane performance were investigated. As the ratio increased, the cloud point shifted to lower temperatures, and the membrane structure changed from a cellular structure due to liquid–liquid phase separation to a particulate structure due to polymer crystallization. Better pore connectivity was obtained in the hollow‐fiber membrane when the ratio of 1,3‐propanediol to glycerol was 50:50, and the membrane showed about 100 times higher water permeability than the membrane prepared with pure glycerol. For the prepared hollow‐fiber membrane, the solute 20 nm in diameter was almost rejected. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 219–225, 2005
Non‐Fickian vapor sorption kinetics in rubbery poly(dimethyl silamethylene) and the effect of radiation‐induced crosslinking
Authors: Alentiev A. , Semenova S. I. , Sanopoulou M. 
Published: November 2004

AbstractSuccessive sorption runs of n‐hexane vapor in supported amorphous poly(dimethyl silamethylene) films at 25°C are presented and discussed. Deviations from Fickian sorption kinetics were observed, and they were more distinct in the as‐prepared films and in the lower n‐hexane vapor pressure runs. Polymer films, γ‐irradiated to produce various degrees of crosslinking, exhibited closer conformity to Fickian sorption kinetics and enhanced diffusivity of n‐hexane vapor. These results were consistent with the presence in the as‐prepared polymer of some labile structural order (undetectable by differential scanning calorimetry or X‐ray diffraction), which tended to be eliminated by radiation‐induced crosslinking or strong swelling. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 226–230, 2005
Arktische Gänse am Niederrhein: Die Exkursion
Authors: Kremer Bruno P. 
Published: November 2004

AbstractHinreißende Naturerlebnisse assoziiert man oft nur mit weit entfernten Regionen oder gar anderen Kontinenten. Manchmal bietet die Natur jedoch selbst im dicht besiedelten Nordrhein‐Westfalen ein beeindruckendes saisonales Schauspiel, das Fachleute ebenso begeistert wie Naturfreunde und sogar Medieninteresse weckt: Von Oktober bis Februar halten sich Zehntausende von Wildgänsen aus der Arktis im Niederrheingebiet auf, um hier zu überwintern.
Rekombinante Antikörper: Werkzeuge gegen Krebs, Infektionen und Autoimmunerkrankungen?
Authors: Dübel Stefan , Rohrbach Petra , Schmiedl Andreas 
Published: November 2004

AbstractIn den vergangenen Jahren stieg sowohl das wissenschaftliche als auch das kommerzielle Interesse an rekombinanten Antikörpern vor allem aufgrund des einfachen Weges zur Gewinnung komplett humaner IgGs. Innovative Selektionsmethoden wie das “phage display” ermöglichen heute sogar die Isolierung von humanen Antikörpern gegen giftige oder stark pathogene Antigene, wie sie unser eigenes Immunsystem nicht hervorzubringen vermag. Der Einsatz der Gentechnik ermöglicht die Herstellung kleiner Immunglobulinfragmente, welche die notwendige Information eines Antikörpers zur Antigenbindung auf ein Minimum reduzieren. Diese Fragmente können dann zu multivalenten, hochaffinen Molekülen mit optimierten pharmakokinetischen Eigenschaften aufgebaut werden, was ihre Anwendungsmöglichkeiten in Forschung, Diagnostik und Therapie gegenüber herkömmlichen Immunglobulinen stark erweitert.
Purpurbakterien aus der Winogradsky‐Säule: Biotechnologie in der Schule
Authors: Scherr Dietmar , Werel Rainer 
Published: November 2004

AbstractSchwefelfreie Purpurbakterien lassen sich leicht aus einer Winogradsky‐Säule entnehmen und in Reinkultur erhalten. Sie eignen sich hervorragend zum Studium des bakteriellen Stoffwechsels, insbesondere der bakteriellen anoxigenen Fotosynthese und deren biotechnologischer Anwendung zur Gewinnung des regenerativen Energieträgers Wasserstoff.
Homo sapiens und die acht Zwerge
Authors: Kronberg Inge 
Published: November 2004

AbstractEs war einmal eine Region im fernen Indonesien, dort lebten neben Drachen und kleinen Elefanten acht Zwerge und ihre Angehörigen. Sie stellten Werkzeuge her und gingen auf die Jagd. Ob moderne Menschen ihnen jemals begegneten, wissen wir nicht. Und weil die Zwerge eines Tages ausstarben, finden wir heute nur noch ihre Überreste...
Parkinson: Toxische Fehlfaltung von Eiweißen
Authors: Kahle Philipp 
Published: November 2004

AbstractDie Parkinson’sche Krankheit ist die häufigste neurodegenerative Bewegungsstörung und betrifft vor allem ältere Menschen. Die Symptome lassen sich einige Zeit lindern, aber die Parkinson‐Krankheit ist nach wie vor unheilbar. Neuere molekulargenetische Erkenntnisse deuten auf multifaktorielle Ursachen für diese Krankheit hin und bieten therapeutische Ansatzpunkte.
Das Vorstellungsgespräch
Authors: Debus‐Spangenberg Isolde 
Published: November 2004

AbstractHaben Sie die Einladung für ein Vorstellungsgespräch auf dem Tisch, ist die größte Hürde im Bewerbungsmarathon überwunden. Zumindest Ihre fachlichen Qualifikationen haben das Unternehmen bereits überzeugt. Immerhin hat man Sie aus meist unzähligen Kandidaten herausgefiltert und will Sie persönlich kennen lernen. Die Medienwissenschaftlerin und Karriereberaterin Isolde Debus‐Spangenberg gibt im letzten Teil unserer Serie Tipps für ein erfolgreiches Vorstellungsgespräch.
Ivan Petrovič Pavlov erhält den Nobelpreis für Physiologie
Authors: Penzlin Heinz 
Published: November 2004

AbstractMit dem russischen Gelehrten Ivan Petrovič Pavlov erhielt erstmalig in der Geschichte ein Physiologe den “Nobelpreis für Physiologie oder Medizin”. Vor ihm hatten die Mediziner Emil Adolf von Behring (1901), Ronald Ross (1902) und Niels Ryberg Finsen (1903) diesen begehrten Preis in Empfang nehmen können. In der Begründung zur Auszeichnung Pavlovs hieß es: “In Anerkennung seiner Arbeit über die Physiologie der Verdauung, die das Wissen über wesentliche Aspekte dieses Bereichs verbessert und erweitert hat.”
Marine Genomik: Erstes Genom einer Diatomee sequenziert
Authors: Valentin Klaus 
Published: November 2004

AbstractDas noch sehr junge Gebiet der Marinen Genomik erlebt seinen ersten eukaryotischen Höhepunkt: Das Genom der Kieselalge Thalassiosira pseudonana (Abbildung 1) wurde in den USA komplett sequenziert. Eine Fülle an interessanten Genen und Stoffwechselwegen wurde entdeckt und die Hinweise verdichten sich, dass der Ursprung der Diatomeen‐Plastiden bei den Rotalgen liegt.
Is There a “Most Chiral Tetrahedron”?
Authors: Rassat André , Fowler Patrick W. 
Published: November 2004

AbstractA degree of chirality is a function that purports to measure the amount of chirality of an object: it is equal for enantiomers, vanishes only for achiral or degenerate objects and is similarity invariant, dimensionless and normalisable to the interval [0,1]. For a tetrahedron of non‐zero three‐dimensional volume, achirality is synonymous with the presence of a mirror plane containing one edge and bisecting its opposite, and hence it is easy to design degree‐of‐chirality functions based on edge length that incorporate all constraints. It is shown that such functions can have largest maxima at widely different points in the tetrahedral shape space, and by incorporation of appropriate factors, the maxima can be pushed to any point in the space. Thus the phrase “most chiral tetrahedron” has no general meaning: any chiral tetrahedron is the most chiral for some legitimate choice of degree of chirality.
The incremental effect of positron emission tomography on diagnostic accuracy in the initial staging of esophageal carcinoma
Authors: Kato Hiroyuki , Miyazaki Tatsuya , Nakajima Masanobu , Takita Junko , Kimura Hitoshi , Faried Ahmad , Sohda Makoto , Fukai Yasuyuki , Masuda Norihiro , Fukuchi Minoru , Manda Ryokuhei , Ojima Hitoshi , Tsukada Katsuhiko , Kuwano Hiroyuki , Oriuchi Noboru , Endo Keigo 
Published: November 2004

AbstractBACKGROUNDThe purpose of the current study was to assess whether [18F]fluorodeoxyglucose positron emission tomography (FDG‐PET) provides incremental value (e.g., additional information on lymph node involvement or the presence of distant metastases) compared with computed tomography (CT) in patients with esophageal carcinoma.METHODSThe authors examined 149 consecutive patients with thoracic esophageal carcinoma. Eighty‐one patients underwent radical esophagectomy without pretreatment, 17 received chemoradiotherapy followed by surgery, 3 underwent endoscopic mucosal resection, and the remaining 48 patients received definitive radiotherapy and chemotherapy. The diagnostic accuracy of FDG‐PET and CT was evaluated at the time of diagnosis.RESULTSThe primary tumor was visualized using FDG‐PET in 119 (80%) of 149 patients. Regarding lymph node metastases, FDG‐PET had 32% sensitivity, 99% specificity, and 93% accuracy for individual lymph node group evaluation and 55% sensitivity, 90% specificity, and 72% accuracy for lymph node staging evaluation. PET exhibited incremental value over CT with regard to lymph node status in 14 of 98 patients who received surgery: 6 patients with negative CT findings were eventually shown to have lymph node metastases (i.e., they had positive PET findings and a positive reference standard [RS]); 6 patients with positive CT findings were shown not to have lymph node metastases (i.e., they had negative PET findings and a negative RS); and 2 patients were shown to have cervical lymph node metastases in addition to mediastinal or abdominal lymph node metastases. Among the remaining patients, PET showed incremental value over CT with regard to distant organ metastases in six patients. The overall incremental value of PET compared with CT with regard to staging accuracy was 14% (20 of 149 patients).CONCLUSIONSFDG‐PET provided incremental value over CT in the initial staging of esophageal carcinoma. At present, combined PET‐CT may be the most effective method available for the preoperative staging of esophageal tumors. Cancer 2005. © 2004 American Cancer Society.
Society of Urologic Oncology position statement: Redefining the management of hormone‐refractory prostate carcinoma
Authors: Chang Sam S. , Benson Mitchell C. , Campbell Steven C. , Crook Juanita , Dreicer Robert , Evans Christopher P. , Hall M. Craig , Higano Celestia , Kelly W. Kevin , Sartor Oliver , Smith Joseph A. 
Published: November 2004

AbstractBecause patients with hormone‐refractory prostate carcinoma are a very diverse group, management of these patients represents a unique challenge. Despite much research, to the authors' knowledge few studies published to date have provided definitive treatment answers. The Society of Urologic Oncology (SUO) convened a multidisciplinary panel of urologists, oncologists, and radiation oncologists to develop a treatment algorithm for patients with hormone‐refractory prostate carcinoma. The resulting treatment outline was based on a review of the literature review and on the expert opinions of the panelists. The current article provided a logical progression of treatment choices that included hormonal manipulations, chemotherapeutic options, and adjunctive therapies. Future clinical trials and therapies were also discussed by the authors. Management strategies should be targeted toward the individual patient. Although significant progress has been made in understanding and treating hormone‐refractory prostate carcinoma, earlier interventions would be ideal and better therapeutic approaches to prolong survival are necessary. Cancer 2005. © 2004 American Cancer Society.
Overproduction of BCR‐ABL induces apoptosis in imatinib mesylate‐resistant cell lines
Authors: Desplat Vanessa , Belloc Francis , Lagarde Valérie , Boyer Catherine , Melo Junia V. , Reiffers Josy , Praloran Vincent , Mahon François‐Xavier 
Published: November 2004

AbstractBACKGROUNDImatinib mesylate, a BCR‐ABL tyrosine kinase inhibitor, induces apoptosis in chronic myeloid leukemia cells. Resistance to imatinib is currently the most important concern of this treatment. One of the main mechanisms of this resistance is overexpression of BCR‐ABL.METHODSIn the current study, the authors investigated the correlation between BCR‐ABL overexpression and apoptosis in BaF/BCR‐ABL and LAMA84 cell lines resistant to imatinib suddenly deprived of the inhibitor, and compared with their sensitive counterpart.RESULTSRemoval of imatinib from culture medium led to a decrease in Bcr‐Abl protein expression by Day 5, which was sustained for ≥ 3 weeks of imatinib deprivation. Apoptosis was observed after 3 days of imatinib deprivation in resistant lines accompanied by caspase activation, loss of membrane asymmetry (annexin V staining), and alteration of mitochondrial potential (dihexyloxacarbocyanine iodide [DiOC6]). Transient activation of the STAT5/Bcl‐xL pathway and Akt kinase activity preceded these responses.CONCLUSIONSThus, imatinib removal led to apoptosis of BCR‐ABL–overexpressing leukemic cells, a phenomenon that could be exploited to sensitize imatinib‐resistant cells to the cytotoxic effect of other drugs. Cancer 2005. © 2004 American Cancer Society.
Differential subcellular localization of cholesterol, gangliosides, and glycosaminoglycans in murine models of mucopolysaccharide storage disorders
Authors: McGlynn Robert , Dobrenis Kostantin , Walkley Steven U. 
Published: November 2004

AbstractThe mucopolysaccharidoses (MPSs) are a complex family of lysosomal storage disorders characterized by failure to degrade heparan sulfate (HS) and/or other types of glycosaminoglycans (GAGs) secondary to the absence of specific lysosomal enzymes. An accompanying storage of glycosphingolipids (GSLs), most notably GM2 and GM3 gangliosides, has also been documented to occur in many types of MPS disease and is believed to be caused by secondary inhibition of GSL‐degradative enzymes by intracellular GAG accumulation. We have documented the presence of secondary ganglioside accumulation in mouse models of several MPS disorders (types I, IIIA, IIIB, and VII) and report that this storage is accompanied by sequestration of free cholesterol in a manner similar to that observed in primary gangliosidoses. Using confocal microscopy, we evaluated the cellular distribution of cholesterol, GM2 and GM3 gangliosides, and HS in brains of mice with MPS IIIA disease. Unexpectedly, we found that although both gangliosides often accumulated in the same neurons, they were consistently located in separate populations of cytoplasmic vesicles. Additionally, GM3 ganglioside only partially co‐localized with the primary storage material (HS), and cholesterol likewise only partially co‐localized with the GM2 and GM3 gangliosides. These findings raise significant questions about the mechanism(s) responsible for secondary accumulation of storage materials in MPS disease. Furthermore, given that GSLs and cholesterol are constituents of membrane rafts believed critical in signal transduction events in neurons, their co‐sequestration in individual neurons suggests the presence of defects in the composition, trafficking, and/or recycling of raft components and thus possible new mechanisms to explain neuronal dysfunction in MPS disorders. J. Comp. Neurol. 480:415–426, 2004. © 2004 Wiley‐Liss, Inc.