bluuebunny/crossref_metadata_2025_split huggingface dataset:
AMR Threat Perception Assessment of Heterotrophic Bacteria From Shrimp Aquaculture Through Epidemiological Cut off Values
Authors: Nadella Ranjit Kumar , Panda Satyen Kumar , Kumar Anuj , Uchoi Devananda , Kishore Pankaj , Badireddy Madhusudana Rao , Kuricheti Pani Prasad , Raman Ram Prakash , Mothadaka Mukteswar Prasad 
Published: February 2024

Abstract

Background
Emergence and dissemination of antibiotic resistance is one of the major risks associated with the rampant usage of antibiotics in food-producing animals including aquaculture.

Objective
To determine Epidemiological Cut-OFF (ECOFF) values of heterotrophic bacterial populations from shrimp culture environments against five different antibiotics

Methods
In this present study, bacterial samples were isolated from Penaeus vannamei culture environment in different locations of Andhra Pradesh, which is the aquaculture hub of India. The bacterial isolates were assessed for antibiotic resistance towards five antibiotics belonging to different classes (oxytetracycline, chloramphenicol, erythromycin, ciprofloxacin, and co-trimoxazole) by the disc diffusion method. Determination of Epidemiological Cut-OFF (ECOFF) values and analysis by employing normalized resistance interpretation (NRI) was carried out.

Results
The most dominant bacterial populations from shrimp culture were Vibrio spp. (pathogenic bacteria) followed by Bacillus spp. (probiotic bacteria). The bacterial isolates showed highest resistance towards oxytetracycline (overall 23.38%) and in location L6 (59.4%) followed by co-trimoxazole (31.1%). ECOFF values calculated by employing NRI showed that the disc diffusion data were distributed in a normalized manner. The maximum ECOFF value was obtained for ciprofloxacin (23.32 mm), while the minimum value was observed for oxytetracycline (9.05 mm). The antibiotic resistant phenotypes showed that the majority of the heterotrophic bacterial isolates (&gt;60%) belonged to the non-wild type phenotype and primarily towards oxytetracycline (90%).

Conclusion
The presence of non-wild antibiotic-resistant phenotypes of heterotrophic bacterial populations (which include not only pathogenic bacteria but also probiotic bacteria) indicates that shrimp culture ponds may be a reservoir for drug-resistant bacteria and there is a greater risk associated with transmission of resistant genes across bacterial flora.

Highlights
NRI analysis of antibiotic disc diffusion data of heterotrophic bacterial populations in shrimp aquaculture environments revealed that majority of them belonged to non-wild type (90%) paticularly to oxytetracycline in comparison to other studied antibiotics (chloramphenicol, erythromycin, ciprofloxacin and co-trimoxazole).
Nitrogen and energy utilization and methane emissions of sheep grazing on annual pasture vs. native pasture
Authors: Xie Kaili , Liu Fuyao , Zhang Cheng , Hou Fujiang 
Published: February 2024

Abstract
The purpose of this study was to evaluate the differences in annual pasture and native pasture on dry matter (DM) intake, nutrient digestibility, nitrogen (N) and energy utilization, and methane (CH4) emission of grazing sheep, and to provide the basis for rational livestock grazing in salinized regions. The study used 10 male Hu sheep ♀ × thin-tailed Han sheep ♂ rams (20 ± 5 kg) aged 5 mo. Sheep grazing was conducted in annual pasture and native pasture using a 2 × 2 Latin square design. After a 15-d adaptation period for grazing, the digestion and metabolism experiment of sheep were conducted, while CH4 emissions were measured using sulfur hexafluoride tracer gas. DM intake did not differ between annual pasture and native pasture (P = 0.386). Meanwhile, the digestibility of DM (P &lt; 0.001), neutral detergent fiber (P &lt; 0.001), acid detergent fiber (P &lt; 0.01), crude protein (P &lt; 0.001), and ether extract (P &lt; 0.001) of sheep grazing on native pasture was significantly higher than that of annual pasture. Sheep grazing on native pasture had increased N intake (P &lt; 0.001) and N retained (P &lt; 0.001) compared with those grazing on annual pasture. Digestion energy (P &lt; 0.05) and metabolic energy (P &lt; 0.01) of sheep grazing on annual pasture were significantly improved compared with those on native pasture, while fecal energy (P &lt; 0.001), urine energy (P &lt; 0.001) and CH4 energy (CH4-E) output (P &lt; 0.001) and CH4 emission (P &lt; 0.001) of sheep grazing on annual pasture were significantly decreased. The CH4-E/gross energy (GE) values of sheep grazing on annual pasture and native pasture were 0.09 and 0.10, respectively. In conclusion, grazing sheep have higher N utilization on native pasture, whereas grazing sheep have higher energy utilization and low CH4 emissions in annual pasture. In conclusion, annual pasture has a lower CH4-E/GE compared to native pasture, which helps in reducing environmental pollution.
Relationship of tissue dimensions and three captive bolt application sites on cadaver heads from mature swine (Sus scrofa domesticus) &lt;200 kg body weight
Authors: Anderson Karly N , Kirk Ashlynn A , Berger Jennifer , Reyes Arquimides A , Woiwode Ruth , Zhitnitskiy Perle E , Vogel Kurt D 
Published: February 2024

Abstract
Penetrating captive bolt (PCB) is a common method of euthanasia for swine but has not been evaluated for mature swine &lt; 200 kg body weight (BW). The objectives were to determine tissue depth, brain contact plane, and visible brain tissue damage (brain damage[BD]) for the common FRONTAL (F) and alternative TEMPORAL (T) and BEHIND EAR (BE) placements for PCB use on sows and boars weighing &lt; 200 kg. Cadaver heads were obtained from 30 sows and 30 boars (estimated BW, mean ± SD; sows: 165.8 ± 22.4 kg; boars: 173.6 ± 21.4 kg) from a slaughter establishment after electrical stunning and exsanguination. Heads were cooled at 2 to 4 °C for approximately 64 h. A Jarvis PAS-Type P 0.25R PCB with a Long Stunning Rod Nosepiece Assembly and a 3.5 GR power load was used for all PCB applications at the following placements: F–3.5 cm superior to the optic orbits at midline, T–at the depression posterior to the lateral canthus of the eye within the plane between the lateral canthus and the base of the ear, or BE–directly caudal to the pinna of the ear on the same plane as the eyes and targeting the middle of the opposite eye. For sows, the bolt path was in the brain for 10/10 (100.0%, 95% CI: 69.2% to 100.0%) F, T, and BE heads. In heads that could reliably be assessed for BD, BD was detected in 10/10 (100.0%, 95% CI: 69.2% to 100.0%) F heads, 9/9 (100.0%, 95% CI: 66.4% to 100.0%) T heads, and 0/10 (0.0%, 95% CI: 0.0% to 30.1%) BE heads. For boars, the bolt path was in the plane of the brain for 8/9 (88.9%, 95% CI: 51.8% to 99.7%) F heads, 9/10 (90.0%, 95% CI: 55.5% to 99.7%) T heads, and 11/11 (100.0%, 95% CI: 71.5% to 100.0%) BE heads. In heads that could reliably be assessed for BD, BD was detected in 8/9 (88.9%, 95% CI: 51.7% to 99.7%) F heads, 7/10 (70.0%, 95% CI: 34.8% to 93.3%) T heads, and 4/11 (36.4%, 95% CI: 10.9% to 69.2%) BE heads. Tissue depth was reported as mean ± SE followed by 97.5% one-sided upper reference limit (URL). For sows, total tissue thickness differed (P &lt; 0.05) between placements (F: 49.41 ± 2.74 mm, URL: 70.0 mm; T: 62.83 ± 1.83 mm, URL: 76.6 mm; BE: 84.63 ± 3.67 mm; URL: 112.3 mm). Total tissue thickness differed (P &lt; 0.05) between placements for boars (F: 54.73 ± 3.23 mm, URL: 77.6 mm; T: 70.72 ± 3.60 mm, URL: 96.3 mm; BE: 92.81 ± 5.50 mm; URL: 135.3 mm). For swine between 120 and 200 kg BW, the F placement may have the greatest likelihood for successful euthanasia due to the least total tissue thickness and may present less risk for failure than the T and BE placements.
Strategies and Challenges of a Safe Fireplace Program in Rural Kenya: A Qualitative Study
Authors: Hunter Mary A , Sylvester Kimutai , Rencken Camerin , White Russell E , Harrington David T , Vivier Patrick M , Parker Robert K 
Published: February 2024

Abstract
There is a lack of information on effective burn prevention strategies. The objective of this study was to examine a safe fireplace program, as a method of burn prevention, in a resource-limited setting. We conducted a qualitative, phenomenological study at a community health and development program for a rural population in Kenya. Semistructured, in-depth interviews were conducted with a purposive sample of community health workers involved with the safe fireplace program. Data were reviewed iteratively and coded independently by two researchers using NVivo 12. The study included five participants. Reflections from participants fell into three main domains: (1) effective methods of education, (2) strategies to incorporate stakeholders, and (3) implementation challenges. The results of this study emphasize the importance of community engagement in implementing a successful burn injury prevention program in a resource-limited setting. The participants involved in this study reported that rather than focusing on resources outside the community, effective methods of education and strategies for incorporating stakeholders depended on involving peers and community leaders. The challenges to the program were similarly specific to community concerns about resources and maintenance. These findings provide information to guide future community health programs in creating successful models for burn prevention strategies in resource-limited areas.
Ocular Chemical Burns: Epidemiological Trends in a Colombian Medical Center
Authors: Robayo-Velásquez Juan F , Bonilla-Escobar Francisco J , Salamanca Omar , Martínez-Blanco Alexander M 
Published: February 2024

Abstract
Ocular chemical burns are a significant cause of visual impairment. This study aims to characterize the ocular chemical burns in a southwestern Colombia referral center and identify associated factors with low vision. A retrospective cohort study was carried out based on the review of medical records of patients diagnosed with chemical eye burns who consulted the emergency ophthalmology service between January 2016 and December 2019. Descriptive statistics were performed. Associations with low vision (Best-Corrected Visual Acuity, ≥20/70 in Snellen chart) in the last follow-up appointment were identified using a multivariate logistic regression with odds ratios (OR) and 95% confidence intervals (95% CI). About 219 eyes affected by chemical burns were identified from 174 patients with a mean age of 39 years (SD ± 20), of which 57.5% (n = 100) were men. We ran a multivariate model adjusted by sex, eye wash, type of chemical, and Dua’s classification. We found that the odds of low vision for patients without eyewash before the consult were 3 times the odds of those who had it (adjusted OR [aOR] = 3.5, 95% CI = 1.3-9.4) and almost 5 times for those with Dua’s classification &gt;1 (aOR = 4.7, 95% CI = 1.7-12.9). The ocular chemical burns reported in this study occurred more in young people of productive age. Acids were the principal causal agent. The association between lack of early management and the severity of the chemical burn with low vision has been highlighted. Ocular burns remain a relevant cause of consultation on the ophthalmology service in Cali, and prevention strategies are required.
Matrix metalloproteinase-9 deficiency confers resilience in fibrodysplasia ossificans progressiva in a man and mice
Authors: Lounev Vitali , Groppe Jay C , Brewer Niambi , Wentworth Kelly L , Smith Victoria , Xu Meiqi , Schomburg Lutz , Bhargava Pankaj , Al Mukaddam Mona , Hsiao Edward C , Shore Eileen M , Pignolo Robert J , Kaplan Frederick S 
Published: February 2024

Abstract
Single case studies of extraordinary disease resilience may provide therapeutic insight into conditions for which no definitive treatments exist. An otherwise healthy 35-year-old man (patient-R) with the canonical pathogenic ACVR1R206H variant and the classic congenital great toe malformation of fibrodysplasia ossificans progressiva (FOP) had extreme paucity of post-natal heterotopic ossification (HO) and nearly normal mobility. We hypothesized that patient-R lacked a sufficient post-natal inflammatory trigger for HO. A plasma biomarker survey revealed a reduction in total matrix metalloproteinase-9 (MMP-9) compared to healthy controls and individuals with quiescent FOP. Whole exome sequencing identified compound heterozygous variants in MMP-9 (c.59C &gt; T, p.A20V and c.493G &gt; A, p.D165N). Structural analysis of the D165N variant predicted both decreased MMP-9 secretion and activity that were confirmed by enzyme-linked immunosorbent assay and gelatin zymography. Further, human proinflammatory M1-like macrophages expressing either MMP-9 variant produced significantly less Activin A, an obligate ligand for HO in FOP, compared to wildtype controls. Importantly, MMP-9 inhibition by genetic, biologic, or pharmacologic means in multiple FOP mouse models abrogated trauma-induced HO, sequestered Activin A in the extracellular matrix (ECM), and induced regeneration of injured skeletal muscle. Our data suggest that MMP-9 is a druggable node linking inflammation to HO, orchestrates an existential role in the pathogenesis of FOP, and illustrates that a single patient’s clinical phenotype can reveal critical molecular mechanisms of disease that unveil novel treatment strategies.
Giant cell arteritis associated with intravenous zoledronic acid administration
Authors: Balbach Meridith L , Hewlett Jennifer R , Wermers Robert A , Warrington Kenneth J , Tanner S Bobo , Chew Erin Y 
Published: February 2024

Abstract
Bisphosphonates frequently provoke a cytokine-driven acute clinical response (ACR) characterized by fever, chills, arthralgias, and myalgias. More rarely, an association between aminobisphosphonates, such as alendronate and zoledronic acid, and rheumatologic and/or immune-mediated syndromes (RIMS) has been described. Herein we report 2 patients, one with a prior history of rheumatic disease and one without, who developed giant cell arteritis meeting the American College of Rheumatology 2022 criteria following zoledronic acid infusion. We subsequently review existing mechanistic and clinical literature supporting this link. The duration of symptoms and elevation of inflammatory markers may serve as indicators for differentiating between the more common ACR and less frequent but potentially morbid RIMS. Although the benefit of bisphosphonates will outweigh the risk of RIMS for most patients with high fracture risk, clinicians should be aware of this phenomenon to assist earlier diagnosis and treatment in affected individuals.
Generative artificial intelligence and building design: early photorealistic render visualization of façades using local identity-trained models
Authors: Jo Hayoung , Lee Jin-Kook , Lee Yong-Cheol , Choo Seungyeon 
Published: February 2024

Abstract
This paper elucidates an approach that utilizes generative artificial intelligence (AI) to develop alternative architectural design options based on local identity. The advancement of AI technologies has increasingly piqued the interest of the architecture, engineering, construction, and facility management industry. Notably, the topic of “visualization” has gained prominence as a means for enhancing communication related to a project, especially in the early phases of design. This study aims to enhance the ease of obtaining design images during initial phases of design by drawing from multiple texts and images. It develops an additional training model to generate various design alternatives that resonate with the identity of the locale through the application of generative AI to the façade design of buildings. The identity of a locality in cities and regions is the capacity for the cities and regions to be identified and recognized as a specific area. Among the various visual elements of urban and regional landscapes, the front face of buildings may play a significant role in people’s aesthetic perception and overall impression of the local environment. The research proposes an approach that transcends the conventional employment of three-dimensional modeling and rendering tools by readily deriving design alternatives that consider this local identity in commercial building remodeling. This approach allows for financial and temporal efficiency in the design communication phase of the initial architectural design process. The implementation and utilization of the proposed approach’s supplementary training model in this study proceeds as follows: (i) image data are collected from the target area using open-source street-view resources and preprocessed for conversion to a trainable format; (ii) textual data are prepared for pairing with preprocessed image data; (iii) additional training and outcome testing are performed using varied text prompts and images; and (iv) the ability to generate building façade images that reflect the identity of the collected locale by using the additional trained model is determined, as evidenced by the findings of the proposed application method study. This enables the generation of design alternatives that integrate regional styles and diverse design requirements for buildings. The training model implemented in this study can be leveraged through weight adjustments and prompt engineering to generate a greater number of design reference images, among other diverse approaches.
Developing lygus bug control strategies in potatoes: plant variety, vertical distribution effect, and insecticide efficacy studies
Authors: Rondon Silvia I , Shrestha Govinda 
Published: February 2024

Abstract
The Columbia Basin of Oregon and Washington is one of the most productive potatoes, Solanum tuberosum L., growing regions in the United States affected by numerous insect pests. Lygus bugs, Lygus spp. (Hemiptera: Miridae), are an increasing problem in potatoes. In 2015, after an outbreak of lygus bugs in potatoes in the Columbia Basin, potato producers used multiple applications of insecticides to control lygus bugs. However, it is poorly researched whether lygus bugs can cause economic damage to the crop. Therefore, our objectives were (i) to determine lygus bugs presence in potato plants, (ii) to determine damage on most commonly grown potato varieties (e.g., Alturas Russet, Ranger Russet, Umatilla Russet, Russet Burbank, and Clearwater Russet), (iii) to determine the number of insecticide applications needed to control lygus bugs, and (iv) to estimate the relationship between lygus bug density and potato yield loss. This study demonstrated that the lygus complex is widely present in the Columbia Basin, Lygus spp. prefers the upper 1/3 section of potato plants in all varieties tested, and the number of applications throughout a field season does not affect yield regardless of variety.
A novel insecticide, isocycloseram, shows promise as an alternative to chlorpyrifos against a direct pest of peanut, Diabrotica undecimpunctata howardi (Coleoptera: Chrysomelidae)
Authors: Bekelja Kyle M , Malone Sean , Mascarenhas Victor , Taylor Sally 
Published: February 2024

Abstract
Larvae of the southern corn rootworm (SCR) Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae) are primary pests of peanut in the Virginia-Carolina region of the United States, and are relatively sporadic pests in southern states such as Georgia, Alabama, and Florida. Peanuts have strict quality standards which, when they are not met, can diminish crop value by more than 65%. Management of direct pests like SCR is therefore crucial to maintaining the economic viability of the crop. The soil-dwelling nature of SCR larvae complicates management due to difficulties associated with monitoring and predicting infestations. Nonchemical management options are limited in this system; preventative insecticide applications are the most reliable management strategy for at-risk fields. Chlorpyrifos was the standard product for larval SCR management in peanut until its registration was revoked in 2022, leaving no effective chemical management option for larvae. We tested a novel insecticide, isocycloseram, for its ability to reduce pod scarring, pod penetration, and non-SCR pod damage in field studies conducted in Suffolk, Virginia in 2020–2022. Overall injury was low in 2020 and 2022, and in 2022 there was not a significant effect of treatment. In 2021, 2 simulated chemigation applications of isocycloseram in July significantly reduced pod scarring and overall pod injury relative to chlorpyrifos and the untreated control. Our results suggest that isocycloseram may become an effective option for managing SCR in peanut, although more work is needed to understand the mechanisms by which it is effective as a soil-applied insecticide.
On DeFi and On-Chain CeFi: How (Not) to Regulate Decentralized Finance
Authors: Schuler Katrin , Cloots Ann Sofie , Schär Fabian 
Published: February 2024

ABSTRACT
This article proposes a framework to assess the factual decentralization of blockchain-based financial infrastructure, commonly referred to as ‘decentralized finance’ (DeFi). It discusses various centralization vectors along the DeFi architecture layers from a technological and legal point of view. Distinguishing between endogenous and inherited centralization, the importance of decentralization assessments for regulators is demonstrated. First, centralization vectors are a strong indication that a DeFi project may have (some) custodial properties warranting regulation. Second, centralization vectors reveal the avenues of control in projects, pointing out appropriate regulatory hooks. The article serves as an interdisciplinary introduction that analyses blockchain-based financial infrastructure and provides policymakers and regulators with a tool to identify the differences between truly independent, neutral infrastructure and fake decentralization. It concludes that the former may have very beneficial properties and can contribute towards a more open and transparent financial system, while the latter is a blockchain-based form of financial intermediation and should be regulated as such.
Differentiated sex chromosomes, karyotype evolution, and spontaneous triploidy in carphodactylid geckos
Authors: Pensabene Eleonora , Augstenová Barbora , Kratochvíl Lukáš , Rovatsos Michail 
Published: February 2024

Abstract
Geckos exhibit derived karyotypes without a clear distinction between macrochromosomes and microchromosomes and intriguing diversity in sex determination mechanisms. We conducted cytogenetic analyses in six species from the genera Nephrurus, Phyllurus, and Saltuarius of the gecko family Carphodactylidae. We confirmed the presence of a female heterogametic system with markedly differentiated and heteromorphic sex chromosomes in all examined species, typically with the W chromosome notably larger than the Z chromosome. One species, Nephrurus cinctus, possesses unusual multiple Z1Z1Z2Z2/Z1Z2W sex chromosomes. The morphology of the sex chromosomes, along with repetitive DNA content, suggests that the differentiation or emergence of sex chromosomes occurred independently in the genus Phyllurus. Furthermore, our study unveils a case of spontaneous triploidy in a fully grown individual of Saltuarius cornutus (3n = 57) and explores its implications for reproduction in carphodactylid geckos. We revealed that most carphodactylids retain the putative ancestral gekkotan karyotype of 2n = 38, characterized by predominantly acrocentric chromosomes that gradually decrease in size. If present, biarmed chromosomes emerge through pericentric inversions, maintaining the chromosome (and centromere) numbers. However, Phyllurus platurus is a notable exception, with a karyotype of 2n = 22 chromosomes. Its eight pairs of biarmed chromosomes were probably formed by Robertsonian fusions of acrocentric chromosomes. The family underscores a remarkable instance of evolutionary stability in chromosome numbers, followed by a profound transformation through parallel interchromosomal rearrangements. Our study highlights the need to continue generating cytogenetic data in order to test long-standing ideas about reproductive biology and the evolution of genome and sex determination.