bluuebunny/crossref_metadata_2025_split huggingface dataset:
The centrality of redox regulation and sensing of reactive oxygen species in abiotic and biotic stress acclimatization
Authors: Denjalli Ibadete , Knieper Madita , Uthoff Jana , Vogelsang Lara , Kumar Vijay , Seidel Thorsten , Dietz Karl-Josef 
Published: February 2024

Abstract
During land plant evolution, the number of genes encoding for components of the thiol redox regulatory network and the generator systems of reactive oxygen species (ROS) expanded, tentatively indicating that they have a role in tailored environmental acclimatization. This hypothesis has been validated both experimentally and theoretically during the last few decades. Recent developments of dynamic redox-sensitive GFP (roGFP)-based in vivo sensors for H2O2 and the redox potential of the glutathione pool have paved the way for dissecting the kinetics changes that occur in these crucial parameters in response to environmental stressors. The versatile cellular redox sensory and response regulatory system monitors alterations in redox metabolism and controls the activity of redox target proteins, and thereby affects most, if not all, cellular processes ranging from transcription to translation and metabolism. This review uses examples to describe the role of the redox- and ROS-dependent regulatory network in realising the appropriate responses to diverse environmental stresses. The selected case studies concern different environmental challenges, namely excess excitation energy, the heavy metal cadmium and the metalloid arsenic, nitrogen or phosphate shortages as examples for nutrient deficiency, wounding, and nematode infestation. Each challenge affects the redox-regulatory and ROS network, but our present state of knowledge also points toward pressing questions that remain open in relation to the translation of redox regulation to environmental acclimatization.
The ‘Candidatus Phytoplasma ziziphi’ effectors SJP1/2 negatively control leaf size by stabilizing the transcription factor ZjTCP2 in jujube
Authors: Ma Fuli , Zheng Yunyan , Zhang Ning , Deng Mingsheng , Zhao Meiqi , Fu Gongyu , Zhou Junyong , Guo Chenglong , Li Yamei , Huang Jinqiu , Sun Qibao , Sun Jun 
Published: February 2024

Abstract
Phytoplasmas manipulate host plant development to benefit insect vector colonization and their own invasion. However, the virulence factors and mechanisms underlying small-leaf formation caused by jujube witches’ broom (JWB) phytoplasmas remain largely unknown. Here, effectors SJP1 and SJP2 from JWB phytoplasmas were identified to induce small-leaf formation in jujube (Ziziphus jujuba). In vivo interaction and expression assays showed that SJP1 and SJP2 interacted with and stabilized the transcription factor ZjTCP2. Overexpression of SJP1 and SJP2 in jujube induced ZjTCP2 accumulation. In addition, the abundance of miRNA319f_1 was significantly reduced in leaves of SJP1 and SJP2 transgenic jujube plants and showed the opposite pattern to the expression of its target, ZjTCP2, which was consistent with the pattern in diseased leaves. Overexpression of ZjTCP2 in Arabidopsis promoted ectopic leaves arising from the adaxial side of cotyledons and reduced leaf size. Constitutive expression of the miRNA319f_1 precursor in the 35S::ZjTCP2 background reduced the abundance of ZjTCP2 mRNA and reversed the cotyledon and leaf defects in Arabidopsis. Therefore, these observations suggest that effectors SJP1 and SJP2 induced small-leaf formation, at least partly, by interacting with and activating ZjTCP2 expression both at the transcriptional and the protein level, providing new insights into small-leaf formation caused by phytoplasmas in woody plants.
Cosmic evolution early release science survey (CEERS): multiclassing galactic dwarf stars in the deep JWST/NIRCam
Authors: Holwerda B W , Hsu Chih-Chun , Hathi Nimish , Bisigello Laura , de la Vega Alexander , Haro Pablo Arrabal , Bagley Micaela , Dickinson Mark , Finkelstein Steven L , Kartaltepe Jeyhan S , Koekemoer Anton M , Papovich Casey , Pirzkal Nor , Cook Kyle , Robertson Clayton , Casey Caitlin M , Aganze Christian , Pérez-González Pablo G , Lucas Ray A , Jogee Shardha , Wilkins Stephen , Burgarella Denis , Kirkpatrick Allison 
Published: February 2024

ABSTRACT
Low-mass (sub)stellar objects represent the low end of the initial mass function, the transition to free-floating planets and a prominent interloper population in the search for high-redshift galaxies. To what accuracy can photometry only classify these? JWST/NIRCam has several advantages over Hubble Space Telescope (HST)/WFC3 near-infrared (NIR): more filters, a greater wavelength range, and greater spatial resolution. Here, we present a catalogue of (sub)stellar dwarfs identified in the cosmic evolution early release science survey (CEERS). We identify 518 stellar objects down to mF200W 28 using half-light radius, a full three magnitudes deeper than typical HST/WFC3 images. A k-means nearest neighbour (kNN) algorithm identifies and types these sources, using four HST/WFC3 and four NIRCam filters, trained on IRTF/spex spectra of nearby brown dwarfs. The kNN with four neighbours classifies well within two subtypes: e.g. M2±2, achieving ∼95 per cent precision and recall. In CEERS, we find 9 M8±2, 2 L6±2, 1 T4±2, and 15 T8±2. We compare the observed long wavelength NIRCam colours – not used in the kNN – to those expected for brown dwarf atmospheric models. The NIRCam F356W−F444W and F410M−F444W colours are redder by a magnitude for the type assigned by the kNN, hinting at a wider variety of atmospheres for these objects. We find a 300–350 pc scale height for M6±2 dwarfs plus a second structural component and a 150–200 pc scale height for T6±2 type dwarfs, consistent with literature values. A single M8±2 dwarf is spectroscopically confirmed at 4 kpc distance.
Performance analysis and optimization of solar multiconjugate adaptive optics systems
Authors: Ke Zibo , Zhang Lanqiang , Yang Ying , Rao Changhui 
Published: February 2024

ABSTRACT
Multiconjugate adaptive optics (MCAO) stands as an essential technology for the development of future large-aperture solar telescopes. Its primary objective is to empower telescopes to achieve nearly diffraction-limited performance while substantially extending the correction field of view (FoV). Conventional solar MCAO relies on the combination of adaptive optics and high-altitude correction (AO + HAC) modules for multiconjugate correction. However, this architectural approach excels in correction performance primarily at the central position, with performance deteriorating as one moves farther from the centre. Consequently, it results in poor consistency of FoV correction performance. To address these limitations, a new architectural approach was introduced, which combines ground layer AO with HAC (GLAO + HAC). Preliminary results have shown that, compared to AO + HAC, this approach significantly enhances FoV correction uniformity. Building upon these initial findings, this paper undertakes a more extensive research of the GLAO + HAC system. Its objective is to compare various solar MCAO system architectures, including AO + HAC, GLAO + HAC, and general MCAO, to finally propose optimization tailored to GLAO + HAC. Through this analysis, the paper conducts the performance comparison between GLAO + HAC and general MCAO. It underscores that, under equivalent configuration parameters, the differences between these two systems are marginal. However, due to the advantage of the independent control of dual correction modules in GLAO + HAC, it can introduce an optimization strategy by increasing the number of subapertures at the cost of reducing the GLAO guide star sensing FoV. Finally, the results of this strategy demonstrate an obvious enhancement in performance and FoV correction consistency within the GLAO + HAC system.
Search for brown dwarfs in IC 1396 with Subaru HSC: interpreting the impact of environmental factors on substellar population
Authors: Gupta Saumya , Jose Jessy , Das Swagat R , Guo Zhen , Damian Belinda , Prakash Prem , Samal Manash R 
Published: February 2024

ABSTRACT
Young stellar clusters are predominantly the hub of star formation and hence, ideal to perform comprehensive studies over the least explored substellar regime. Various unanswered questions like the mass distribution in brown dwarf regime and the effect of diverse cluster environment on brown dwarf formation efficiency still plague the scientific community. The nearby young cluster, IC 1396 with its feedback-driven environment, is ideal to conduct such study. In this paper, we adopt a multiwavelength approach, using deep Subaru HSC along with other data sets and machine learning techniques to identify the cluster members complete down to ∼ 0.03 M⊙ in the central 22 arcmin area of IC 1396. We identify 458 cluster members including 62 brown dwarfs which are used to determine mass distribution in the region. We obtain a star-to-brown dwarf ratio of ∼ 6 for a stellar mass range 0.03–1 M⊙ in the studied cluster. The brown dwarf fraction is observed to increase across the cluster as radial distance from the central OB-stars increases. This study also compiles 15 young stellar clusters to check the variation of star-to-brown dwarf ratio relative to stellar density and ultraviolet (UV) flux ranging within 4–2500 stars pc−2 and 0.7–7.3 G0, respectively. The brown dwarf fraction is observed to increase with stellar density but the results about the influence of incident UV flux are inconclusive within this range. This is the deepest study of IC 1396 as of yet and it will pave the way to understand various aspects of brown dwarfs using spectroscopic observations in future.
Health care utilization after enrollment in an outpatient structured clinical program for children with medical complexity
Authors: Cook Stacey , Hall Matt , Stringfellow Isabel , Berry Jay G 
Published: February 2024

Abstract

Objectives
Although children with medical complexity (CMC) with high health resource utilization use outpatient structured clinical programs (SCP) to optimize their health, little is known about variation in trends of their health service use shortly after enrollment. We measured these trends and assessed the utility of patient characteristics to predict them.

Methods
Retrospective analysis of 506 CMC newly enrolled in an outpatient, academic SCP. We measured outpatient and inpatient health service use for the first 6 months following enrollment. Using cluster analysis, we categorized CMC with similar trends by health service. We assessed patient demographic (e.g., age) and clinical [type and number of complex chronic conditions (CCC)] characteristics with the cluster categories.

Results
Most (90.3%) CMC enrolled had ≥1 CCCs; 53.8% had ≥3 CCCs. For all CMC, outpatient specialty visits, phone calls, and hospitalizations (not involving ICU) decreased significantly over the first 6 months after enrollment. For example, the hospitalization rate decreased from 9.7% to 4.5% in the 1st and 6th months, respectively, (P &lt; 0.001). Cluster analysis revealed four categories of hospitalization trends, 6 months after enrollment: 72.9% of CMC had no hospital use; 16.0% had increased then decreased use; 8.3% had decreased use; and 2.8% had increased use. No patient and clinical characteristics clearly distinguished which hospitalization trend CMC experienced.

Conclusions
Although the overall population of CMC experienced decreased outpatient and inpatient health services utilization over time, this decrease was not uniformly experienced. Fundamental patient demographic and clinical characteristics did not predict health service trends.
Independent metabolism of oligosaccharides is the keystone of synchronous utilization of cellulose and hemicellulose in Myceliophthora
Authors: Liu Jia , Chen Meixin , Gu Shuying , Fan Rui , Zhao Zhen , Sun Wenliang , Yao Yonghong , Li Jingen , Tian Chaoguang 
Published: February 2024

Abstract
The effective utilization of cellulose and hemicellulose, the main components of plant biomass, is a key technical obstacle that needs to be overcome for the economic viability of lignocellulosic biorefineries. Here, we firstly demonstrated that the thermophilic cellulolytic fungus Myceliophthora thermophila can simultaneously utilize cellulose and hemicellulose, as evidenced by the independent uptake and intracellular metabolism of cellodextrin and xylodextrin. When plant biomass serviced as carbon source, we detected the cellodextrin and xylodextrin both in cells and in the culture medium, as well as high enzyme activities related to extracellular oligosaccharide formation and intracellular oligosaccharide hydrolysis. Sugar consumption assay revealed that in contrast to inhibitory effect of glucose on xylose and cellodextrin/xylodextrin consumption in mixed-carbon media, cellodextrin and xylodextrin were synchronously utilized in this fungus. Transcriptomic analysis also indicated simultaneous induction of the genes involved in cellodextrin and xylodextrin metabolic pathway, suggesting carbon catabolite repression (CCR) is triggered by extracellular glucose and can be eliminated by the intracellular hydrolysis and metabolism of oligosaccharides. The xylodextrin transporter MtCDT-2 was observed to preferentially transport xylobiose and tolerate high cellobiose concentrations, which helps to bypass the inhibition of xylobiose uptake. Furthermore, the expression of cellulase and hemicellulase genes was independently induced by their corresponding inducers, which enabled this strain to synchronously utilize cellulose and hemicellulose. Taken together, the data presented herein will further elucidate the degradation of plant biomass by fungi, with implications for the development of consolidated bioprocessing-based lignocellulosic biorefinery.
Epidemiology of antiphospholipid syndrome: macro- and microvascular manifestations
Authors: Gaspar Pedro , Sciascia Savino , Tektonidou Maria G 
Published: February 2024

AbstractAntiphospholipid syndrome (APS) is a systemic autoimmune disease characterized by thrombotic and non-thrombotic macro- and microvascular manifestations and pregnancy complications in the setting of persistent antiphospholipid antibodies (aPL), namely anticardiolipin antibodies, anti-β2 glycoprotein-I antibodies and lupus anticoagulant. Four decades after its first description, APS prevalence and incidence are still not completely understood due to the limited number of well-designed, population-based multi-ethnic studies. Furthermore, despite decades of efforts to standardise aPL immunoassays, considerable intraassay and interlaboratory variances in aPL measures still exist. Large multicentre APS cohorts have shown a 10-year survival of ∼91% and the presence of catastrophic APS occurs in about 1% of the entire population, associated with a 50% mortality rate. Clinically, any organ can be affected in the context of large, medium or small vessel (artery and/or vein) thrombosis. Macrovascular thrombosis is the hallmark of the disease and veins are more frequently affected than arteries. Deep vein thrombosis/pulmonary embolism thromboembolic disease is the most common APS manifestation, while stroke and transient ischaemic attack are the most frequent arterial thrombosis events. Myocardial infarction can also occur and contributes to increased mortality in APS. A minority of patients present with thrombosis affecting the intraabdominal organs, including the liver, spleen, small and large bowel, and the kidneys. Microvascular thrombosis, including APS nephropathy, chronic skin ulcers and livedoid vasculopathy represent a diagnostic challenge requiring histologic confirmation. In this narrative review we summarize the available evidence on APS epidemiology, focusing on the description of the prevalence of macro- and microvascular manifestations of the disease.
Comparison of retention of biologics in Japanese patients with elderly-onset rheumatoid arthritis—the ANSWER cohort study
Authors: Jinno Sadao , Onishi Akira , Hattori Shuhei , Dubreuil Maureen , Ueda Yo , Nishimura Keisuke , Okano Takaichi , Yamada Hirotaka , Yamamoto Wataru , Murata Koichi , Onizawa Hideo , Ebina Kosuke , Maeda Yuichi , Son Yonsu , Amuro Hideki , Hara Ryota , Hata Kenichiro , Shiba Hideyuki , Katayama Masaki , Watanabe Ryu , Hashimoto Motomu , Saegusa Jun 
Published: February 2024

Abstract

Objectives
This multicentre, retrospective study aimed to compare retention and reasons for discontinuation between Janus kinase inhibitors (JAKi) and biologic DMARDs in patients with elderly-onset rheumatoid arthritis (EORA).

Methods
Patients with RA enrolled in a Japanese multicentre observational registry between 2015 and 2022 were included. EORA was defined as RA with onset at age 60 or over. To adjust confounding factors by indication for initiation of TNF inhibitors (TNFi), IL-6 inhibitors (IL-6i), cytotoxic T-lymphocyte associated antigen 4 immunoglobulin (CTLA4-Ig) blockers, or JAKi, a propensity score based on baseline characteristics was used to compare drug retention. To assess the reasons for discontinuation, retention rates for ineffectiveness, adverse events and remission were analysed as secondary outcomes.

Results
A total of 572 patients with 835 treatment courses were identified (314 TNFi, 175 IL-6i, 228 CTLA4-Ig and 118 JAKi). After adjusting for differences in baseline characteristics, drug retention was significantly higher for IL-6i (hazard ratio [HR] = 0.38; 95% CI: 0.27, 0.55; P &lt; 0.01) as compared with TNFi. Discontinuation due to lack of effectiveness was lower with JAKi (HR = 0.38; 95% CI: 0.22, 0.66; P &lt; 0.01) and IL-6i (HR = 0.29; 95% CI: 0.19, 0.46; P &lt; 0.01) as compared with TNFi although CTLA4-Ig had a similar HR to TNFi. The adjusted incidence of discontinuation due to adverse events was higher for JAKi (HR = 2.86; 95% CI: 1.46, 5.59; P &lt; 0.01) than for TNFi.

Conclusion
In EORA patients, IL-6i and JAKi had longer retention and less discontinuation due to ineffectiveness than TNFi. The potential risks of JAKi should be approached with an individualized perspective.
Arthroplasty rates and risk in familial Mediterranean fever patients: a large population-based study
Authors: Ben-Shabat Niv , Fisher Lior , Maixner Nitzan , Arow Mohamad , Ozeri David J , Patt Yonatan Shneor , Watad Abdulla , Amital Howard , Gendelman Omer 
Published: February 2024

Abstract

Background
FMF is a genetic disorder characterized by recurrent episodes of fever and inflammation in various organs, including the joints. Traditionally, the arthritis of FMF has been considered relatively harmless. However, anecdotal evidence has suggested that it may contribute to long-term joint damage, which may necessitate surgical joint replacement. This study aimed to investigate the rates of arthroplasty among FMF patients and compare it with those of the general population.

Methods
The study used the electronic database of the largest health-care organization in Israel to identify 9769 FMF patients diagnosed between 2000 and 2016. A similar number of age-, gender-, and residency-matched controls were also identified. The rates of arthroplasty were compared between the two groups. A logistic regression model predicting the need for arthroplasty within the FMF group was formed to identify potential risk factors.

Results
Of the 9769 FMF patients, 114 (1.2%) underwent arthroplasty, compared with 64 (0.7%) of the control group [unadjusted odds ratio (OR) = 1.79, 95% CI 1.32–2.43; partially adjusted OR = 1.97, 95% CI 1.40–2.77; fully adjusted OR = 1.92, 95% CI 1.35–2.72]. Within the FMF cohort, those of North African origin had a significantly higher risk of arthroplasty (OR = 6.89, 95% CI 5.09–9.33; P &lt; 0.001).

Conclusion
FMF patients can experience long-term joint damage that may require arthroplasty. Although this complication is relatively uncommon in FMF patients, it occurs almost twice as frequently as compared with the general population. FMF patients of North African origin are at an even higher risk.
Innate Immunity Activation in Newly Diagnosed Ileocolonic Crohn’s Disease: A Cohort Study
Authors: Angriman Imerio , Bordignon Giovanni , Kotsafti Andromachi , Mescoli Claudia , Scarpa Melania , Ruffolo Cesare , Fassan Matteo , Dei Tos Angelo Paolo , D’Incà Renata , Savarino Edoardo , Zingone Fabiana , Pucciarelli Salvatore , Bardini Romeo , Castagliuolo Ignazio , Scarpa Marco 
Published: February 2024

BACKGROUND:
Recent studies showed that early surgery for Crohn’s disease leads to a lower recurrence rate. However, the underlying mechanism is unknown.

OBJECTIVE:
The study aims to analyze the innate immunity microenvironment in ileal mucosa according to Crohn’s disease duration.

DESIGN:
A prospective cohort study.

SETTINGS:
Tertiary referral center for IBD surgery.

PATIENTS:
A total of 88 consecutive Crohn’s disease patients undergoing ileocolonic resection were prospectively enrolled. Mucosal samples were obtained from both healthy and inflamed ileum. Data from a public dataset were analyzed as an external validation cohort.

MAIN OUTCOME MEASURES:
Neutrophil infiltration was evaluated at histology and macrophage subpopulation at immunohistochemistry. Expression of TLR2, TLR4, TLR5, DEFB1, DEFB4A, DEFB103, DEFA5 and DEFA6 was quantified by Real-Time qPCR. Concentrations of BDNF, CCL11, ICAM1, IL1A, IL1B, IL1RN, IL12 p40, IL12 p70, IL15, IL17A, IL23A, MMP3, CCL3, KITLG, VEGFA were determined with immunometric assay.

RESULTS:
Neutrophil infiltration is inversely correlated with disease duration. DEFB4A mRNA expression tended to be higher in late-stage Crohn’s disease (p = 0.07). A higher number of macrophages expressed CD163 at low intensity in the late stage (p = 0.04). The concentration of IL15 (p = 0.02) and IL23A (p = 0.05) was higher in healthy ileal mucosa of early-stage patients. In the external cohort, expression of DEFB1 (p = 0.03) and DEFB4A (p = 0.01), IL2 (p = 0.04), and IL3 (p = 0.03) increased in late-stage patients.

LIMITATIONS:
A relatively small number of patients, especially in the newly diagnosed group.

CONCLUSIONS:
In newly diagnosed Crohn’s disease, high levels of IL-15 and IL-23 in healthy mucosa suggest that innate immunity is the starter of acute inflammation. Moreover, M2 macrophages increase in healthy mucosa of late-stage Crohn’s disease patients suggesting that reparative and profibrotic processes are predominant in the long term and in this phase, anti-inflammatory therapy may be less efficient. See Video Abstract.