bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ‐ILF2 in oral squamous cell carcinoma promotes cisplatin resistance and induces M2 polarization of macrophages
Authors: Wu Siyuan , Lv Xiaozhi , Wei Haigang , Chen Wuya , Zheng Junming , Li Xia , Song Jing , Ai Yilong , Zou Chen 
Published: October 2023

AbstractCisplatin (CDDP) chemoresistance is one of the predominant factors in oral squamous cell carcinoma (OSCC) treatment failure. Uncovering the mechanisms underlying CDDP resistance is of great importance in OSCC therapy. Circular RNAs (circRNAs) are a newly discovered class of noncoding RNAs, which are reported to participate in the progression of various diseases, including cancer. However, the function of circRNAs in CDDP resistance in OSCC remains unclear. Quantitative reverse transcription PCR was used to search for different circRNAs between OSCC cell lines and CDDP‐resistant cell lines. The results showed that circ‐ILF2 expression was higher in CDDP‐resistant OSCC cell lines. The stability of circ‐ILF2 was also confirmed using RNase R and actinomycin D assays. Functional experiments, including cytotoxicity, apoptosis and growth rate assays, showed that upregulation of circ‐ILF2 contributes to CDDP resistance. Luciferase reporter‐gene, RNA pull‐down and quantitative real‐time PCR (RT‐qPCR) assays showed that circ‐ILF2 functions as a microRNA sponge for miR‐1252. Luciferase reporter assays, RNA pull‐down, RT‐qPCR and Western blotting showed that miR‐1252 directly targeted and regulated the expression of KLF8. Circ‐ILF2 plays an important role in CDDP resistance in OSCC. Circ‐ILF2 exerts its function through the miR‐1252/KLF8 pathway. In addition, tumour‐associated macrophages (TAM) play important roles in cancer progressions, our results showed that circ‐ILF2 in OSCC cells induced the M2 polarization of macrophages which provided new thoughts on immunotherapy. Our results suggest that circ‐ILF2 may represent a potential therapeutic target in CDDP‐resistant OSCC.
Novel, pathogenic insertion variant of GSDME associates with autosomal dominant hearing loss in a large Chinese pedigree
Authors: Cheng Jingliang , Li Ting , Tan Qi , Fu Jiewen , Zhang Lianmei , Yang Luquan , Zhou Baixu , Yang Lisha , Fu Shangyi , Linehan Alora Grace , Fu Junjiang 
Published: October 2023

AbstractNonsyndromic hearing loss (NSHL) is a genetically diverse, highly heterogeneous condition characterised by deafness, and Gasdermin E (GSDME) variants have been identified as directly inducing autosomal dominant NSHL. While many NSHL cases associated with GSDME involve the skipping of exon 8, there is another, less understood pathogenic insertion variant specifically found in Chinese pedigrees that causes deafness, known as autosomal dominant 5 (DFNA5) hearing loss. In this study, we recruited a large Chinese pedigree, conducted whole‐exome and Sanger sequencing to serve as a comprehensive clinical examination, and extracted genomic DNA samples for co‐segregation analysis of the members. Conservation and expression analyses for GSDME were also conducted. Our clinical examinations revealed an autosomal dominant phenotype of hearing loss in the family. Genetic analysis identified a novel insertion variant in GSDME exon 8 (GSDME: NM_004403.3: c.1113_1114insGGGGTGCAGCTTACAGGGTGGGTGT: p. P372fs*36). This variant is segregated with the deafness phenotype of this pedigree. The GSDME gene was highly conserved in the different species we analysed, and its mRNA expression was ubiquitously low in different human tissues. In conclusion, we have successfully identified a novel pathogenic insertion variant of GSDME in a Chinese pedigree that causes deafness, shedding light on the genetic basis of hearing loss within this specific family. Our findings expand the spectrum of known variants associated with GSDME‐related deafness and may further support both the underlying gain‐of‐function mechanism and functional associations of GSDME hearing loss variants.
Analysis of the correlation between skin barrier function and age in rosacea patients in Qinghai region
Authors: Qi Zixuan , Wang Feng , Huang Yongmei , Wang Ping 
Published: October 2023

AbstractObjectiveThis study aims at investigating the difference in facial skin barrier function between rosacea patients and the healthy population of different ages in the Qinghai region and its correlation with age, providing a basis for clinical nursing and treatment.MethodsThe data of 216 rosacea patients were collected, and 211 healthy testers were selected as the control group. The skin barrier characteristics of rosacea patients of different ages were evaluated by comparing transepidermal water loss (TEWL), stratum corneum hydration (SCH), sebum content (SC), and pH values between the two groups. Then, the correlation between skin barrier function and age in rosacea patients and the healthy population was analyzed.ResultsIn all four age groups, the TEWL of the rosacea group was higher than that of the healthy control group, and the epidermal hydration was lower than the healthy control group. In the population aged over 40, the sebum content in rosacea was lower than that in the healthy control group. In the age group of 40–59, the pH value of the rosacea group was higher than the healthy control group. In both the rosacea and the control groups, TEWL was positively correlated with age, and epidermal hydration was negatively correlated with age. The sebum content in rosacea group was negatively correlated with age (p < 0.05).ConclusionAs age increases, the facial epidermal function of both rosacea patients and the healthy population declines. Therefore, with increasing age, attention should be paid to enhance the epidermal function to slow down the skin aging process.
Vitamin D supplementation can enhance therapeutic effects of excimer laser in patients with vitiligo
Authors: Kim Jin Cheol , Kim Hye Ran , Park Jin Seo , Lee So Yeon , Kim Hye One , Park Chun Wook , Chung Bo Young 
Published: October 2023

AbstractPurposeVitiligo is a disease of acquired depigmentation characterized by the destruction of melanocytes. A theoretical association between low level of 25‐hydroxyvitamin D [25(OH)D] and vitiligo has been previously suggested. The objective of this study was to determine the efficacy of intramuscular injection of cholecalciferol with excimer laser compared with the excimer laser alone for vitiligo treatment.MethodsThis study included 26 patients diagnosed with non‐segmental vitiligo and low serum 25(OH)D levels (<20 ng/mL). The participants were randomly divided into two groups through randomization. The treatment using a 308‐nm excimer laser was administered to both groups, and the study group additionally received cholecalciferol injection.ResultsThe Vitiligo Area Scoring Index (VASI) scores showed an 83.6% improvement over the initial score in the study group, whereas the control group showed a 54.7% improvement after 6 months of treatment. After 6 months of treatment, the study group showed a significantly higher proportion of patients who achieved VASI50 and VASI75 compared with the control group.ConclusionIntramuscular injection of cholecalciferol can be a supplemental option for the treatment of vitiligo patients with vitamin D deficiency with excimer laser therapy.
Selectivity of chemical and biological foliar treatments on the phyllosphere communities of bacteria and fungi antagonistic to Fusarium verticillioides in maize
Authors: Perrony Paul Esteban Pherez , Guimarães Rafaela Araújo , Reis Luisa Oliveira , Gomes Larissa Bitencourt , da Silva Leonardo José , de Albuquerque Correa César Murilo , da Silva Júlio Carlos Pereira , de Medeiros Flávio Henrique Vasconcelos 
Published: October 2023

AbstractMaize grain production in the tropics is affected by Fusarium verticillioides, leading to significant losses in yield and quality. Fungicides are commonly used to control foliar diseases and increase grain yield, but have limited efficacy against F. verticillioides and may even stimulate the fungus to produce mycotoxins. Biocontrol can reduce F. verticillioides populations and fumonisin, but little is known about the resulting microbial interactions on the phylloplane and their relationship with F. verticillioides. This study aimed at evaluating the impact of different foliar treatments on the cultivable phylloplane community of fungi and bacteria and their potential in controlling F. verticillioides in maize. Results showed that two sprays of Bacillus subtilis BIOUFLA2 increased the percentage of native phylloplane antagonist bacteria and fungi against F. verticillioides by 25.0% and 27.3%, respectively, compared with the water control. However, two sprays with a triazole + strobilurin fungicide at different phenological stages in maize reduced such antagonistic communities. From field trials, 34 bacteria and 13 fungi from the phylloplane were selected for their inhibitory activity against F. verticillioides, with most coming from the exclusive treatment with BIOUFLA2. The study also revealed that the biocontrol agent‐recruited phylloplane indigenous lineages act through antibiosis, competition, and parasitism. Therefore, the adoption of biocontrol to limit F. verticillioides population build‐up not only has a direct activity on the pathogen but also recruits fungal and bacterial antagonists that may act additively or synergistically with the applied biocontrol agent.
Investigating seed bank potential of crustose coralline algae using DNA metabarcoding
Authors: van der Reis Aimee L. , Sewell Mary A. , Nelson Wendy A. 
Published: October 2023

AbstractTo examine the potential for the autogenic ecosystem engineers, crustose coralline algae (CCA), to serve as seed banks or refugia for life stages of other species, it is critical to develop sampling protocols that reflect the diversity of life present. In this pilot study on two shallow water species of CCA collected from Raoul Island (Kermadec Islands; Rangitāhua) New Zealand, we investigated two preservation methods (ethanol vs. silica gel), sampled inner and outer regions of the crusts, and used DNA metabarcoding and seven genes/gene regions (16S rRNA, 18S rRNA, 23S rRNA, cox1, rbcL, and tufA genes and the ITS rRNA region) to develop a protocol for taxa identification. The results revealed immense diversity, with typically more taxa identified within the inner layers than the outer layers. As highlighted in other metabarcoding studies and in earlier work on rhodoliths (nodose coralline algae), reference databases are incomplete, and to some extent, the use of multiple markers mitigates this issue. Specifically, the 23S rRNA and rbcL genes are currently more suitable for identifying algae, while the cox1 gene fares better at capturing the diversity present inclusive of algae. Further investigation of these autogenic ecosystem engineers that likely act as marine seed banks is needed.
Promoting high‐quality interactions among early childhood education minority students: a case study of dialogic literary gatherings
Authors: Girbés‐Peco Sandra , Tellado Itxaso , López de Aguileta Garazi , de Botton Fernández Lena 
Published: October 2023

AbstractQuality dialogue and interactions in the classroom are crucial for creating effective learning environments and reducing inequalities from an early age. Dialogic reading interventions are known to be beneficial in early childhood education, but there is still much to learn about creating the most conducive interactions in the classroom. This article focuses on dialogic literary gatherings (DLGs), a successful educational action that introduces classic literature to children. DLGs create a learning context where rich interactions emerge from an egalitarian dialogue, valuing all contributions regardless of the person's position in making inferences. The study analysed instructional, emotional and social interactions in DLGs in an early childhood classroom in a disadvantaged Spanish neighbourhood, using a communicative research methodology. Findings show that DLGs facilitate high‐quality interactions between teachers and students and among students. The pre‐school teacher used DLGs to stretch the learning and thinking of participating 4‐ and 5‐year‐old students, promoting self‐regulation and prosocial behaviours. DLGs can play a vital role in creating a more equitable and stimulating learning environment in early childhood education.
“Kita habis…we will be gone”: The politics of population, family planning and racialization in West Papua
Authors: Rasidjan Maryani Palupy 
Published: October 2023

AbstractIn the context of a steadily decreasing Indigenous population, active military occupation, and a documented history of human rights abuses perpetrated by Indonesian state security forces, Black Indigenous Papuans have uttered phrases like extinction, and we will be gone in public and private spaces. These utterances often follow an indictment of Indonesia's national family planning program as a key node of state apparatuses of domination and, by extension, genocide. Amid Indonesia's global health success story of a historically lauded national family planning model, I examine the emergence of a local pronatalist program in which health workers are both providers and deniers of access to birth control. Through highlighting this story of Indigenous refusal and racial survival in the terrain of women's reproduction the stakes of a necropolitical environment marked by occupation, population control, and fears of genocide are brought into high relief.
Superior approach from the pocket for atrioventricular junction ablation performed at the time of conduction system pacing implantation
Authors: Palmisano Pietro , Parlavecchio Antonio , Crea Pasquale , Guido Alessandro , Accogli Michele , Coluccia Giovanni 
Published: October 2023

AbstractBackgroundConduction system pacing (CSP) and atrioventricular junction ablation (AVJA) improve outcomes in patients with symptomatic, refractory atrial fibrillation (AF). Superior approach (SA) from the pocket via axillary or subclavian vein has been proposed as an alternative to the conventional femoral venous access (FA) to perform AVJA.ObjectiveTo assess the feasibility and safety of SA for AVJA performed simultaneously with CSP, and to compare this approach with FA.MethodsA prospective, observational study, enrolling consecutive patients with symptomatic, refractory AF undergoing simultaneous CSP and AVJA.ResultsA total of 107 patients were enrolled: in 50, AVJA was primarily attempted with SA, in 69 from FA. AVJA with SA was successful in 38 patients (76.0%), while in 12 patients, a subsequent FA was required. AVJA from FA was successful in 68 patients (98.5%), while in one patient, a left‐sided approach via femoral artery was required. Compared with FA, SA was associated with a significantly longer duration of ablation (238.0 ± 218.2 vs. 161.9 ± 181.9 s; p = .035), a significantly shorter procedure time (28.1 ± 19.8 vs. 19.8 ± 16.8 min; p = .018), an earlier ambulation (2.7 ± 3.2 vs. 19.8 ± 0.1 h; p < .001), and an earlier discharge from procedure completion (24.0 ± 2.7 vs. 27.1 ± 5.1 h; p < .001). After a median follow‐up of 12 months, the rate of complications was similar in the two groups (2.0% in SA, 4.3% in FA; p = .483).ConclusionSimultaneous CSP and AVJA with SA is feasible, with a safety profile similar to FA. Compared to FA, this approach reduces the procedure times and allows earlier ambulation and discharge.
Investigating the relationship between specific negative symptoms and metacognitive functioning in psychosis: A systematic review
Authors: McGuire Nicola , Gumley Andrew , Hasson‐Ohayon Ilanit , Allan Stephanie , Aunjitsakul Warut , Aydin Orkun , Bo Sune , Bonfils Kelsey A. , Bröcker Anna‐Lena , de Jong Steven , Dimaggio Giancarlo , Inchausti Felix , Jansen Jens Einar , Lecomte Tania , Luther Lauren , MacBeth Angus , Montag Christiane , Pedersen Marlene Buch , Pijnenborg Gerdina Henrika Maria , Popolo Raffaele , Schwannauer Matthias , Trauelsen Anne‐Marie , van Donkersgoed Rozanne , Wu Weiming , Wang Kai , Lysaker Paul H. , McLeod Hamish 
Published: October 2023

AbstractBackgroundDisrupted metacognition is implicated in development and maintenance of negative symptoms, but more fine‐grained analyses would inform precise treatment targeting for individual negative symptoms.AimsThis systematic review identifies and examines datasets that test whether specific metacognitive capacities distinctly influence negative symptoms.Materials & MethodsPsycINFO, EMBASE, Medline and Cochrane Library databases plus hand searching of relevant articles, journals and grey literature identified quantitative research investigating negative symptoms and metacognition in adults aged 16+ with psychosis. Authors of included articles were contacted to identify unique datasets and missing information. Data were extracted for a risk of bias assessment using the Quality in Prognostic Studies tool.Results85 published reports met criteria and are estimated to reflect 32 distinct datasets and 1623 unique participants. The data indicated uncertainty about the relationship between summed scores of negative symptoms and domains of metacognition, with significant findings indicating correlation coefficients from 0.88 to −0.23. Only eight studies investigated the relationship between metacognition and individual negative symptoms, with mixed findings. Studies were mostly moderate‐to‐low risk of bias.DiscussionThe relationship between negative symptoms and metacognition is rarely the focus of studies reviewed here, and negative symptom scores are often summed. This approach may obscure relationships between metacognitive domains and individual negative symptoms which may be important for understanding how negative symptoms are developed and maintained.ConlclusionMethodological challenges around overlapping participants, variation in aggregation of negative symptom items and types of analyses used, make a strong case for use of Individual Participant Data Meta‐Analysis to further elucidate these relationships.
Optimizing ErCas12a for efficient gene editing in Arabidopsis thaliana
Authors: Pietralla Janine , Capdeville Niklas , Schindele Patrick , Puchta Holger 
Published: October 2023

SummaryThe ErCas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31% sequence homology with the commonly used AsCas12a, its intellectual property may not be covered by the granted patent rights for Cas12a nucleases. Thus, ErCas12a became an attractive alternative for practical applications. However, the editing efficiency of ErCas12a is strongly target sequence‐ and temperature‐dependent. Therefore, optimization of the enzyme activity through protein engineering is especially attractive for its application in plants, as they are cultivated at lower temperatures. Based on the knowledge obtained from the optimization of Cas12a nucleases, we opted to improve the gene editing efficiency of ErCas12a by introducing analogous amino acid exchanges. Interestingly, neither of these mutations analogous to those in the enhanced or Ultra versions of AsCas12a resulted in significant editing enhancement of ErCas12a in Arabidopsis thaliana. However, two different mutations, V156R and K172R, in putative alpha helical structures of the enzyme showed a detectable improvement in editing. By combining these two mutations, we obtained an improved ErCas12a (imErCas12a) variant, showing several‐fold increase in activity in comparison to the wild‐type enzyme in Arabidopsis. This variant yields strong editing efficiencies at 22 °C which could be further increased by raising the cultivation temperature to 28 °C and even enabled editing of formerly inaccessible targets. Additionally, no enhanced off‐site activity was detected. Thus, imErCas12a is an economically attractive and efficient alternative to other CRISPR/Cas systems for plant genome engineering.
Dissociable event‐related potential modulations of intrinsic and extrinsic factors in temporal integration
Authors: Akyürek Elkan G. , Balta Gülşen 
Published: October 2023

AbstractWe investigated visual temporal integration, by which multiple stimuli appearing in rapid succession are perceived as a single event. Temporal integration not only depends intrinsically on the passage of time but also, extrinsically, on the number and distribution of successive stimuli that are presented across that time interval. Here, we used a missing element task to investigate intrinsic and extrinsic factors in temporal integration, by manipulating stimulus duration and number, respectively. We found that both contributed interactively to integration performance and that varying the information rate over time did not further modulate this pattern. Intrinsic and extrinsic factors had dissociable effects on the N1, N2, N2pc, and P3 components of the event‐related potential, implicating unique contributions to perceptual discrimination, spatio‐temporal grouping, attention, and response decision‐making. Stimulus number‐induced effects on the event‐related potential also generally arose later than those of stimulus duration. The latter already modulated the amplitude of the N1 and the early phase of the N2pc, while the former did not. The collective results suggest that while both intrinsic and extrinsic factors drive temporal integration, they do so in different ways. This difference during integration may eventually be reflected in the way in which we perceive longer, episodic events.
Political regime, institutional capacity, and inefficient policy: Evidence from gasoline subsidies
Authors: McAlexander Richard J. , Yang Joonseok , Urpelainen Johannes 
Published: October 2023

AbstractWhy do some governments subsidize gasoline consumption, despite its very high economic and environmental costs? We answer this question by examining how a state's political regime and level of institutional capacity jointly determine its level of fossil fuel price distortion. We find that, without sufficient institutional capacity, democratic regimes do not necessarily provide less fuel subsidies, as those governments are unable to pursue other more efficient welfare policies. Using data on monthly domestic gasoline prices from 2003 to 2015, we demonstrate that democratic governments with high institutional capacity are less likely to control domestic gasoline prices. Democratic institutions and strong institutional capacity jointly mitigate the effect of the benchmark oil price increases on the domestic price. These results suggest that the combination of motive (democratic accountability) and means (institutional capacity) can help countries avoid inefficient subsidy policies.
Linguistic but not minimal group membership modulates spontaneous level‐2 perspective interference in 8‐year‐old children
Authors: Elek Lívia Priyanka , Király Ildikó , Pető Réka , Szücs Renáta , Elekes Fruzsina , Oláh Katalin 
Published: October 2023

AbstractThis paper presents evidence that social categorization affects spontaneous level‐2 visual perspective taking (L2PT) differently depending on the type of social category in 8‐year‐old. In Experiment 1 (N = 46), children were paired with same‐age peers, who belonged to the same or a different minimal group. In Experiment 2 (N = 42) children participated with an adult confederate, who either shared their cultural group membership or was a member of an out‐group (inferred from a linguistic cue, accent). In Experiment 3 (N = 80), children were acting together with an adult confederate who was at the same time a member of the same or a different minimal group and shared their linguistic membership. This allowed us to investigate how these social categories influence each other. Spontaneous L2PT was not affected by the minimal group manipulation. However, accent weakened L2PT when it implied that the task partner belonged to an out‐group. When both category cues were present, accent—that could be an indicator of shared knowledge attribution—played a more pronounced role in attenuating L2PT. It is argued that social categories that are indicative of the partner's knowledge states but not ad hoc groups influence spontaneous mentalizing.