bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Levels of Long Non-coding RNAs NEAT1, GAS5, and GAPLINC
Altered in Rheumatoid Arthritis
Authors: Tofigh Roghayeh , Hosseinpourfeizi Mohammadali , Safaralizadeh Reza , Ghoddusifar Sepideh , Baradaran Behzad 
Published: October 2023

Background:
Rheumatoid arthritis (RA), an autoimmune joint inflammatory disease,
presents a significant challenge due to its prevalence, particularly among women, affecting around
6% of individuals over the age of 65. Novel insights into disease mechanisms are crucial for improved
diagnostic and therapeutic approaches.

Objective:
Long non-coding RNAs (lncRNAs) have emerged as potential contributors to the pathogenesis
of various autoimmune diseases, including RA. This study aims to investigate the unique
roles of four lncRNAs-NEAT1, GAS5, TMEVPG1, and GAPLINC-in the etiology of RA.

Methods:
Leveraging isolated serum samples from RA patients and healthy controls, we comprehensively
evaluated the expression profiles of these lncRNAs.

Results:
Notably, our findings unveil a distinctive landscape of lncRNA expressions in RA.
Among them, GAPLINC exhibited a significantly elevated average expression in the serum samples
of RA patients, suggesting a potential biomarker candidate for disease stratification. Importantly,
reduced expression of NEAT1 and GAS5 was observed in RA patients, highlighting their
possible roles as diagnostic and prognostic markers. Conversely, TMEVPG1 displayed unaltered
expression levels in RA samples.

Conclusion:
Our study introduces a novel dimension to RA research by identifying NEAT1,
GAS5, and GAPLINC as promising serological biomarkers. These findings hold significant clinical
implications, offering potential avenues for improved diagnosis, disease monitoring, and therapeutic
interventions in RA.
Effect of the Theory-driven Educational Intervention on Self-Care Behavior
and Glycosylated Hemoglobin Level in Patients with Type 2 Diabetes
Authors: Pourmohammad Mojgan , Maheri Mina , Khalkhali Hamid Reza , Moghaddam-Tabrizi Fatemeh , Didarloo Alireza 
Published: October 2023

Background and aims:
The diabetics' lack of knowledge about self-care and incorrect
beliefs in this care are the reasons for their admission to hospitals. The present study aimed to
examine the impact of education based on the theory of planned behavior (TPB) on intention,
self-care behavior, and glycosylated hemoglobin (HbA1c) levels in patients with Type 2 Diabetes.

Methods:
The present study was conducted on 60 diabetic patients who were randomly classified
into intervention and control groups. In the intervention group, self-care education based on TPB
was held, but the control group only received routine care. The data were collected using a fourpart
questionnaire including characteristics, items of patients’ knowledge, items of TPB constructs,
and items of diabetes self-care. Data were analyzed in SPSS software.

Results:
In intervention group, mean score of self-care behavior and its domains significantly
changed from (total: 52.26±14.75, diabetic diet: 22.20±6.05, exercise: 5.17±4.04, blood sugar
testing: 5.03 ± 4.15, medication: 5.40±2.28, foot care: 13.47±8.42) into 76.66±8.87, 29.43±5.48,
7.60±2.60, 8.73±2.31, 6.48±1.09, 23.53±3.09, respectively three months after intervention
(p<0.01). In this case, these changes were not seen in the control group. The mean level of
HbA1c in the intervention group (6.38±0.16) was significantly lower than before the intervention
(7.56±1.08) and the control group (8.02±0.25) three months after intervention(p<0.01).

Conclusion:
Education based on the TPB was effective in improving the patients' self-care behavior
and HbA1c index. Therefore, we suggest all medical centers to use the TPB as an effective
and low-cost educational approach to improve diabetics' self-care behavior and health.
Cardiac Amyloidosis: A Contemporary Review of Medical and Surgical
Therapy
Authors: Brownell Drew , Pillai Aiswarya J. , Nair Nandini 
Published: October 2023

Abstract:
Amyloidosis is a systemic disease initiated by deposition of misfolded proteins in the
extracellular space, due to which multiple organs may be affected concomitantly. Cardiac amyloidosis,
however, remains a major cause of morbidity and mortality in this population due to infiltrative
/restrictive cardiomyopathy. This review attempts to focus on contemporary medical
and surgical therapies for the different types of cardiac amyloidosis. Amyloidosis affecting the
heart are predominantly of the transthyretin type (acquired in the older or genetic in the younger
patients), and the monoclonal immunoglobulin light chain (AL) type which is solely acquired. A
rare form of secondary amyloidosis AA type can also affect the heart due to excessive production
and accumulation of the acute-phase protein called Serum Amyloid A” (SAA) in the setting
of chronic inflammation, cancers or autoinflammatory disease. More commonly AA amyloidosis
is seen in the liver and kidney. Other rare types are Apo A1 and Isolated Atrial Amyloidosis
(AANF). Medical therapies have made important strides in the clinical management of the two
common types of cardiac amyloidosis. Surgical therapies such as mechanical circulatory support
and cardiac transplantation should be considered in appropriate patients. Future research using
AI driven algorithms for early diagnosis and treatment as well as development of newer genetic
engineering technologies will drive improvements in diagnosis, treatment and patient outcomes.
A Monocytic Barrier to the Humanization of Immunodeficient Mice
Authors: Du Emily J. , Muench Marcus O. 
Published: October 2023

Abstract:
Mice with severe immunodeficiencies have become very important tools for studying
foreign cells in an in vivo environment. Xenotransplants can be used to model cells from many
species, although most often, mice are humanized through the transplantation of human cells or tissues
to meet the needs of medical research. The development of immunodeficient mice is reviewed
leading up to the current state-of-the-art strains, such as the NOD-scid-gamma (NSG)
mouse. NSG mice are excellent hosts for human hematopoietic stem cell transplants or immune reconstitution
through transfusion of human peripheral blood mononuclear cells. However, barriers
to full hematopoietic engraftment still remain; notably, the survival of human cells in the circulation
is brief, which limits overall hematological and immune reconstitution. Reports have indicated
a critical role for monocytic cells – monocytes, macrophages, and dendritic cells – in the clearance
of xenogeneic cells from circulation. Various aspects of the NOD genetic background that affect
monocytic cell growth, maturation, and function that are favorable to human cell transplantation
are discussed. Important receptors, such as SIRPα, that form a part of the innate immune system
and enable the recognition and phagocytosis of foreign cells by monocytic cells are reviewed.
The development of humanized mouse models has taken decades of work in creating more immunodeficient
mice, genetic modification of these mice to express human genes, and refinement of
transplant techniques to optimize engraftment. Future advances may focus on the monocytic cells
of the host to find ways for further engraftment and survival of xenogeneic cells.
Evaluation of Clinical Characteristics and Outcomes of Chest Trauma Patients with COVID-19 Infection Undergoing Emergency Surgeries
Authors: Ahmadinejad Mojtaba , Soltanian Ali , Pak Haleh , Zolfaghari Maryam , Ahmadinejad Izadmehr 
Published: October 2023

Background:
This study aims to examine the clinical characteristics and prognosis of
patients with chest trauma who were infected with COVID-19 and underwent emergency surgery.

Objective:
In light of the COVID-19 pandemic, this research investigates the impact of covid-19
on patients with chest trauma who underwent emergency surgery.

Methods:
This retrospective cohort study was conducted on 56 patients with chest trauma in two
groups with COVID-19, and no covid who underwent surgery in Shahid Madani Hospital.

Results:
Among the trauma patients, 21 were infected with COVID-19, and 35 were not. The predominant
causes of trauma were car and motorcycle accidents. Among COVID-19 patients, 3.3%
exhibited symptoms of fever, chills, cough, shortness of breath, and gastrointestinal symptoms,
while 5.9% had severe lung involvement confirmed by CT scan. Following surgery, 1.57% (12
individuals) of COVID-19 patients were transferred to the ICU, with an average hospitalization
duration of 1.11 days. In contrast, the average hospitalization duration for non-COVID-19 chest
trauma patients was 1.9 days (p =0.015).

Conclusion:
Patients with chest trauma who underwent surgery and were infected with COVID-19
demonstrated significant differences in average length of stay, ICU admissions, and pneumonia
incidence. However, there was no significant distinction in the mortality rate between the two
groups.
Modeling Molecular Study between SDO1/Inhibitors: Search of New Treatments for Amyotrophic Lateral Sclerosis
Authors: Mancini Daiana Teixeira , Militani Isabela Aparecida , de Castro Alexandre Alves , Assis Letícia Cristina , de Castro Ramalho Teodorico 
Published: October 2023

Background:
The Amyotrophic lateral sclerosis (ALS) is a degenerative and most frequent
motor neuron disease characterized by the progressive impairment of upper and lower motor neurons. The
treatment of the disease is still palliative and limited to the use of only two drugs, riluzole and edaravone,
which only prolong survival by a few months. Taking into account the low number of therapy available
for this disease, identification of novel therapeutic strategies for ALS is urgently needed. The superoxide
dismutase 1 (SOD1) was the first gene in which mutations were found to be causative for the neurodegenerative
disease and has been used as a promising target for the ALS treatment.

Methods:
In this work we used powerful computational tools (in silico method) such as Ligand-based
Virtual Screening (SBVS), docking and molecular dynamics techniques to collaborate with the discovery
of new candidates for more potent drugs to be used in the ALS disease treatment.

Results:
Compound 1 shows good stability in the active site of the SOD1 enzyme, with an intermolecular
interaction energy of -154.80 kcal/mol. In addition, the presence of some amino acids such as Glu24,
Glu21, Pro28, Lys23 and Lys30 is important for to maintain stability of this compound inside SOD1.

Conclusion:
This study was essential due to a low number of therapy available for this disease until the
moment. With this study, it was possible to observe that Compound 1 is the most promising for the design
of SOD1 mutant enzyme potential inhibitors. However, experimental tests in the SOD1 mutant to validate
the inhibitory effect of Compound 1 will be required.
Design, Synthesis, and Pharmacological Evaluation of Novel Tacrine
Derivatives as Multi-target ANTI-Alzheimer’s Agents In Rat Models
Authors: R.S. Remya , N. Ramalakshmi , P. Muralidharan , C.N. Nalini 
Published: October 2023

Background:
Alzheimer’s disease is a progressive neurodegenerative disorder for which no
curative drugs are available and treatment available is just palliative.

Objectives:
Current research focused on design of Tacrine-Flavone hybrids as multitargeted cholinesterase
and monoamine oxidase B inhibitors.

Methods:
A total of 10 Tacrine- Flavone hybrids were designed, synthesized and characterized. The in
vitro neurotoxicity and hepatotoxicity of the synthesized compounds determined using SHSY5Y cell
line and HEPG2 cell line. One most active compound (AF1) with least toxicity in in vitro studies was
chosen for in vivo studies. Acute and subacute toxicity of the novel compound AF1 conducted on
Wistar rats according to OECD guideline 423 and 407. The LD50 value of the novel compound calculated
according to Finney’s method using Probit analysis. Anti-Alzheimer’s activity studies conducted
on male Wistar rats. Behavioral studies conducted and AChE and MAO-B activity determined in rat
brain.

Results and Discussion:
All the compounds exhibited good inhibitory effect on MAO B and AChE.
The neurotoxicity studies of the active compound AF1 did not show toxicity up to 100μg. The hepatotoxicity
study of the most active compound AF1, showed the compound to be safe up to 200μg. The
LD 50 value of the novel compound after a single oral administration was found to be 64 mg/kg bodyweight
in rats. Subacute toxicity studies did not show any remarkable toxicity in the vital organs up
to 40 mg/kg. Activity studies showed comparable results with standard at 20 mg/kg.

Conclusion:
The results showed that the novel Tacrine-Flavone hybrids are multitarget-directed ligands,
which are safe and active compared to tacrine and can be a promising lead molecule for further
study.
Phosphodiesterase 2 and Its Isoform A as Therapeutic Targets in the
Central Nervous System Disorders
Authors: Metkar Sanjay K. , Yan Yuqing , Lu Yue , Lu Jianming , Zhu Xiongwei , Du Fu , Xu Ying 
Published: October 2023

Abstract:
Cyclic adenosine monophosphates (cAMP) and cyclic guanosine monophosphate (cGMP)
are two essential second messengers, which are hydrolyzed by phosphodiesterase's (PDEs), such as
PDE-2. Pharmacological inhibition of PDE-2 (PDE2A) in the central nervous system improves cAMP
and cGMP signaling, which controls downstream proteins related to neuropsychiatric, neurodegenerative,
and neurodevelopmental disorders. Considering that there are no specific treatments for these
disorders, PDE-2 inhibitors' development has gained more attention in the recent decade. There is high
demand for developing new-generation drugs targeting PDE2 for treating diseases in the central nervous
and peripheral systems. This review summarizes the relationship between PDE-2 with neuropsychiatric,
neurodegenerative, and neurodevelopmental disorders as well as its possible treatment, mainly
involving inhibitors of PDE2.
Analysis of Environmental Literacy Profile Indonesian Students at STKIP Pesisir Selatan, West Sumatera
Authors: Arif Muhammad , Maryani Enok 
Published: October 2023

Environmental literacy is considered an achievement of environmental education. Preservice teachers with good environmental literacy knowledge are more likely to align their actions with their understanding. Preservice teachers have two roles: as behavioural models for their students and as members of society. This research is a descriptive study that utilizes the survey method. The subjects of this research are 50 students from the Mathematics Education, Geography Education, and Technology and Informatics Education programs at STKIP Pesisir Selatan, West Sumatera-Indonesia. The data collection instrument in this research is a questionnaire. The data analysis technique used to process knowledge data utilizes the NELA score. This research indicates that students from all three programs have moderate environmental knowledge. The environmental literacy of Geography Education students has a minor standard deviation, showing relatively low variation in the scores. On the other hand, students in the Mathematics Education and Technology and Informatics Education programs have a higher standard deviation, indicating a more significant variation in their science literacy scores. Geography Education students have higher knowledge of ecology and pollution than students from the other two programs. Meanwhile, mathematics education students have the highest scores in environmental problem-solving skills.Â Keywords: Analysis, Environmental Literacy, Preservice Teacher
PENGARUH MODEL PEMBELAJARAN CORE BERBANTUAN MEDIA PHET UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KRITIS SISWA KELAS IV
Authors: Harahap Siti Fatimah , Manurung Imelda Free Unita 
Published: October 2023

Abstract: This study aims to determine the effect of the CORE learning model assisted by phet media to improve the critical thinking skills of fourth grade students. This research is a quantitative research, type of Quasi Experiment type two group pretest-posttest design. The research instrument used was a test in the form of multiple choice of 15 items, a questionnaire and observation. Data analysis techniques using t-test. Statistical test results showed that the average critical thinking result of students using the CORE learning model assisted by phet media was 76.48, while the results of the critical thinking test of students using conventional learning models were 62.74. The results of the t-test obtained 𝑡ℎ𝑖𝑡𝑢𝑛𝑔 > 𝑡𝑡𝑎𝑏𝑒𝑙 which is 3.404>2.024 and the value of Sig.(2-tailed) < 0.05, namely 0.002 < 0.05. it is stated that 𝐻0 is rejected and 𝐻𝑎 is accepted. It was concluded that there was an influence of the CORE learning model assisted by phet media to improve the critical thinking skills of fourth grade elementary school students.Keyword: CORE Learning Model, Critical Thinking Ability. Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh model pembelajran CORE berbantuan media phet untuk meningkatkan keterampilan berpikir kritis siswa kelas IV. Penelitian ini merupakan penelitian kuantitatif, jenis Quasi Experimen tipe two group pretest-posttest design. Instrumen penelitian yang digunakan yaitu tes berupa pilihan berganda sebanyak 15 butir soal, angket dan observasi. Tehnik analisis data menggunakan uji-t. Hasil uji statistik menunjukkan rata-rata hasil berpikir kritis peserta didik menggunakan model pembelajaran CORE berbantuan media phet sebesar 76,48, sedangkan hasil test berpikir kritis peserta didik menggunakan model pembelajaran konvensional sebesar 62,74. Hasil uji-t diperoleh hasil 𝑡ℎ𝑖𝑡𝑢𝑛𝑔 > 𝑡𝑡𝑎𝑏𝑒𝑙 yaitu dan nilai Sig.(2-tailed) < 0.05 yaitu 0.002 < 0.05. dinyatakan bahwa 𝐻0 ditolak dan 𝐻𝑎 diterima. Disimpulkan ada pengaruh model pembelajaran CORE berbantuan media phet untuk meningkatkan keterampilan berpikir kritis siswa SD kelas IV.Kata Kunci: Model Pembelajaran CORE, Kemampuan Berpikir Kritis.
Costs and Revenues Balance: The Case of Municipal Delegated Competences in Slovakia
Authors: Meričková Beáta Mikušová , Svidroňová Mária Murray , Bakoš Eduard , Stejskal Jan 
Published: October 2023

Abstract
Local authorities’ financial resources should be commensurate with the competences provided for by the law. However, in most countries, representatives of local self-governments claim that their total revenues are insufficient to deliver allocated tasks at a standard scale and level of quality. Such statements are somewhat problematic, especially in less-developed countries, where cost-center accounting is not used and service standards are not defined. This paper analyzes the relationship between the costs of delegated competences and the resources provided to cover these costs in Slovakia. We found that Slovak municipalities (with some exceptions) do not know the exact costs of delegated competences, and that the state does not cover the total costs of delegated competences, which contradicts the Slovak constitution. Our short comparison in the discussion shows that the Czech situation is very similar, suggesting that the problem might be common for all post-Soviet countries.
The fauna of Opomyzoidea (excluding Agromyzidae) in the Gemer area (Central Slovakia): new records 2014–2022
Authors: Roháček Jindřich 
Published: October 2023

Abstract
A new biodiversity inventory of opomyzoid families (excl. Agromyzidae) in the Gemer area (Slovakia) is presented with a critical synopsis of previously published data and new records obtained from the Muránska planina National Park and the Cerová vrchovina Protected Landscape Area. The study includes the families Clusiidae (10 species), Acartophthalmidae (2), Opomyzidae (12), Anthomyzidae (14), Aulacigastridae (3), Stenomicridae (2), Periscelididae (5), Asteiidae (7) and Odiniidae (8). For each recorded species remarks on general distribution, biology, faunistic and/or nature conservation importance are provided. Four species, viz. Aulacigaster falcata Papp, 1998 (Aulacigastridae), Periscelis (Myodris) haennii Pollini Paltrinieri & Roháček, 2022 (Periscelididae), Neoalticomerus fabricius Withers & Papp, 2012 and Odinia photophila Papp, 1977 (second known specimen) (both Odiniidae), are recorded from Slovakia for the first time, and 14 other species are new additions to the fauna of the Gemer area, viz. Geomyza acutipennis Czerny, 1928, G. balachowskyi Mesnil, 1934, G. breviseta Czerny, 1928 and G. subnigra Drake, 1992 (Opomyzidae), Anagnota bicolor (Meigen, 1838), Anthomyza collini Andersson, 1976 and Stiphrosoma cingulatum (Haliday, 1855) (Anthomyzidae), Periscelis (Periscelis) annulipes Loew, 1858 (Periscelididae), Asteia elegantula Zetterstedt, 1847 and Leiomyza birkheadi Gibbs, 2007 (Asteiidae), Neoalticomerus formosus (Loew, 1844), Odinia czernyi Collin, 1952, O. meijerei Collin, 1952 and O. trinotata Robineau-Desvoidy, 1830 (Odiniidae). First records of Neoalticomerus fabricius are also given from Germany, Poland and Czech Republic. Except for the above new faunal records, also the following species are considered interesting and important in the Gemer area because of their rarity or significance from the nature conservation point of view: Clusiodes gentilis (Collin, 1912) (Clusiidae), Stiphrosoma humerale Roháček & Barber, 2005 (Anthomyzidae), Podocera soniae (Merz & Roháček, 2005) (Stenomicridae), Periscelis (P.) laszloi Roháček, 2022 and P. (P.) winnertzii Egger, 1862 (Periscelididae). Diagnostic characters of some species of Odiniidae are emphasized and illustrated.
A Deep Reinforcement-Learning-Based Relay Selection for Underwater Sensors Network
Authors: Aftab Muhmmad Waleed , Hussain Sajjad , Husain Aftab , Khan Umar Ali , Kundi Hamza 
Published: October 2023

Abstract
Due to their limited frequency range and fast fading channels, underwater sensor networks (USNs) are vulnerable to collisions of packets. In this paper, we propose a deep reinforcement learning-based relay selection scheme with shortest latency (DRL-SL) for USNs that enables to choose the relay based on the state that comprised of the bit error rate (BER) of the previous transmission, and the jamming power measured by the relay node. The DRL-SL-based relay selection scheme completed in two phases. In the first phase, a deep neural network based learning is performed and second phase is the real-time interaction with the underwater sensor network. Numerical results give the bound on how efficiently the system performs in terms of bit error rate, energy use, and node utility. According to the numerical results, the proposed DRL-SL based relay selection scheme can enhance relay performance in comparison to the benchmark underwater relay techniques.
Design and Development of Healthcare System Using Blockchain
Authors: Adnan Myasar Mundher 
Published: October 2023

Abstract
This entire system stands upon blockchain technology. The system is initially broken down in sections like different modules for patient, doctor, pharmacy and insurance agent. It is completed utilising a personal blockchain from Corda and Ethereum called Ganache as well as a private blockchain. This supports the development, testing, and general deployment of dApps. An Ethereum development framework is called Truffle Suite. Completely control the lifetime of modern contracts that includes bespoke installations, linking with different libraries and intricate applications on Ethereum. In dApps, Meta Mask is usefull for account creation, login and bitcoin transcations. The storage of all huge files and documents is done using the IPFS technology. Because IPFS is a P2P network circuit for decentralised data storage and the produced hash is simple to store on the blockchain, it is used. ReactJS was used to build the system’s front end. The Remix online compiler for Solidity is used to build and test smart and modern contracts that are written in the Solidity blockchain programming language.