bluuebunny/crossref_metadata_2025_split huggingface dataset:
ENSINO SUPERIOR E REGIÃO: UMA PROPOSTA METODOLÓGICA PARA A ANÁLISE DO BRASIL
Authors: Araújo dos Santos Maria Nainam Silvino , De Campos Luis Henrique Romani 
Published: October 2023

O presente artigo trata da expansão recente do ensino superior de cunho federal no Brasil por meio da abertura de novas unidades, ou expansão das já existentes, de Universidades Federais e Institutos Federais. Objetivou-se trazer notas metodológicas que permitem a sua análise. Para tal, partiu-se da observação da metodologia proposta pela OCDE para as questões que se relacionam com as interações entre o Ensino Superior e a(s) Região(ões). Conclusivamente, se percebeu que a metodologia utilizada pela OCDE dista significativamente da realidade brasileira em diferentes pontos. Sendo assim, se propôs que o estudo do caso brasileiro deve-se valer de um conjunto de procedimentos qualitativos que se alinhem a procedimentos quantitativos. Ou seja, a complexidade do tema e do cenário brasileiro caminha na direção de que se devem adotar procedimentos metodológicos que, concatenados, permitam a construção de uma visão holística do objeto de pesquisa.
Predicting the risk of acute respiratory failure among asthma patients—the A2-BEST2 risk score: a retrospective study
Authors: Qi Yanhong , Zhang Jing , Lin Jiaying , Yang Jingwen , Guan Jiangan , Li Keying , Weng Jie , Wang Zhiyi , Chen Chan , Xu Hui 
Published: October 2023

Objectives
Acute respiratory failure (ARF) is a common complication of bronchial asthma (BA). ARF onset increases the risk of patient death. This study aims to develop a predictive model for ARF in BA patients during hospitalization.

Methods
This was a retrospective cohort study carried out at two large tertiary hospitals. Three models were developed using three different ways: (1) the statistics-driven model, (2) the clinical knowledge-driven model, and (3) the decision tree model. The simplest and most efficient model was obtained by comparing their predictive power, stability, and practicability.

Results
This study included 398 patients, with 298 constituting the modeling group and 100 constituting the validation group. Models A, B, and C yielded seven, seven, and eleven predictors, respectively. Finally, we chose the clinical knowledge-driven model, whose C-statistics and Brier scores were 0.862 (0.820–0.904) and 0.1320, respectively. The Hosmer-Lemeshow test revealed that this model had good calibration. The clinical knowledge-driven model demonstrated satisfactory C-statistics during external and internal validation, with values of 0.890 (0.815–0.965) and 0.854 (0.820–0.900), respectively. A risk score for ARF incidence was created: The A2-BEST2 Risk Score (A2 (area of pulmonary infection, albumin), BMI, Economic condition, Smoking, and T2(hormone initiation Time and long-term regular medication Treatment)). ARF incidence increased gradually from 1.37% (The A2-BEST2 Risk Score ≤ 4) to 90.32% (A2-BEST2 Risk Score ≥ 11.5).

Conclusion
We constructed a predictive model of seven predictors to predict ARF in BA patients. This predictor’s model is simple, practical, and supported by existing clinical knowledge.
The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
Authors: Tan Lifan , Li Wensong , Su Qin 
Published: October 2023

Background
NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis.

Methods
To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis.

Results
Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells.

Conclusion
High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment.
Integrative approaches to a revision of the liverwort in genus Aneura (Aneuraceae, Marchantiophyta) from Thailand
Authors: Anantaprayoon Nopparat , Wonnapinij Passorn , Kraichak Ekaphan 
Published: October 2023

Background
The genus Aneura Dumort. is a simple thalloid liverwort with cosmopolitan distributions. Species circumscription is problematic in this genus due to a limited number of morphological traits. Two species are currently reported from Thailand, including A. maxima and A. pinguis. At the global scale, A. pinguis is considered a cryptic species, as the species contains several distinct genetic groups without clear morphological differentiation. At the same time, the identity of A. maxima remains unclear. In this work, we examined the level of diversity of Aneura species found in Thailand using both morphological and molecular data.

Methods
We measured the morphological traits and generated the molecular data (four markers: trnL–F, trnH–psbA, rbcL, and ITS2) from the Thai specimens. The concatenated dataset was then used to reconstruct phylogeny. Species delimitation with GMYC, bPTP, ASAP, and ABGD methods was performed to estimate the number of putative species within the genus.

Results
The samples of A. pinguis formed several clades, while A. maxima sequences from Poland were grouped in their clade and nested within another A. pinguis clade. We could not recover a sample of A. maxima from Thailand, even from the reported locality. Two putative species were detected among Thai Aneura samples. However, no morphological trait could distinguish the specimens from the two observed genetic groups.

Discussion
The previously observed paraphyletic nature of A. pinguis globally was also found among Thai samples, including several putative species. However, we could not confirm the identity of A. maxima from Thai specimens. The previous report could result from misidentification and problematic species circumscription within Aneura. The results highlighted the need to include multiple lines of evidence for the future taxonomic investigation of the group.
Semaphorin4A promotes lung cancer by activation of NF-κB pathway mediated by PlexinB1
Authors: Wei Xiang , Liu Zhili , Shen Yili , Dong Hui , Chen Kai , Shi Xuefei , Chen Yi , Wang Bin , Dong Shunli 
Published: October 2023

Background
Lung cancer (LC) is the most prevalent cancer with a poor prognosis. Semaphorin4A (Sema4A) is important in many physiological and pathological processes. This study aimed to explore the role and mechanism of Sema4A in LC.

Methods
Firstly, Sema4A expression was analyzed by the available dataset and detected in human normal bronchial epithelial cell line (HBE) and LC cell line (NCI-H460). Then, LC cells were transfected with Sema4A siRNA, and the cells were stimulated by PlexinB1, PlexinB2, PlexinD1 blocking antibodies, IgG antibody, BAY 11-7082 (an inhibitor for NF-κB pathway) and Sema4A-Fc protein, alone or in combination. After transfection, PlexinB1 mRNA expression was analyzed. Next, the biological functions, including proliferative, migratory, invasive abilities and viability of the cells were detected by colony formation, scratch, Transwell and MTT assays, respectively. NF-κB, Stat3 and MAPK protein expressions were determined by western blot. Furthermore, the secretion of IL-6 in LC cells was tested by ELISA.

Results
Sema4A was highly expressed in LC tissues and cells, could activate the NF-κB pathway and upregulate PlexinB1 mRNA expression. Furthermore, we observed that Sema4A knockdown suppressed the biological functions of NCI-H460 cells, while Sema4A-Fc protein reversed the situation. However, Sema4A-induced biological functions and activation in the NF-κB pathway were inhibited by PlexinB1 blocking antibody. Consistently, Sema4A promoted IL-6 production, which was down-regulated by PlexinB1 blocking antibody and BAY 11-7082.

Conclusions
Sema4A may facilitate LC development via the activation of the NF-κB pathway mediated by PlexinB1, suggesting that Sema4A would be a novel therapeutic target for LC treatment.
Ultrasound‐Sensitive Intelligent Nanosystems: A Promising Strategy for the Treatment of Neurological Diseases
Authors: Pan Xueting , Huang Wenping , Nie Guangjun , Wang Changyong , Wang Hai 
Published: October 2023

AbstractNeurological diseases are a major global health challenge, affecting hundreds of millions of people worldwide. Ultrasound therapy plays an irreplaceable role in the treatment of neurological diseases due to its noninvasive, highly focused, and strong tissue penetration capabilities. However, the complexity of brain and nervous system and the safety risks associated with prolonged exposure to ultrasound therapy severely limit the applicability of ultrasound therapy. Ultrasound‐sensitive intelligent nanosystems (USINs) are a novel therapeutic strategy for neurological diseases that bring greater spatiotemporal controllability and improve safety to overcome these challenges. This review provides a detailed overview of therapeutic strategies and clinical advances of ultrasound in neurological diseases, focusing on the potential of USINs‐based ultrasound in the treatment of neurological diseases. Based on the physical and chemical effects induced by ultrasound, rational design of USINs is a prerequisite for improving the efficacy of ultrasound therapy. Recent developments of ultrasound‐sensitive nanocarriers and nanoagents are systemically reviewed. Finally, the challenges and developing prospects of USINs are discussed in depth, with a view to providing useful insights and guidance for efficient ultrasound treatment of neurological diseases.
Plasmon‐enhanced FRET biosensor based on Tm3+/Er3+ co‐doped core‐shell upconversion nanoparticles for ultrasensitive virus detection
Authors: Lao Xinyue , Liu Yuan , Li Lihua , Song Menglin , Ma Yingjin , Yang Mo , Chen Guanying , Hao Jianhua 
Published: October 2023

AbstractOutbreaks of infectious viruses offer a formidable challenge to public healthcare systems and early detection of viruses is essential for preventing virus propagation. In this work, an ultrasensitive plasmon‐enhanced fluorescence resonance energy transfer (FRET) biosensor based on core‐shell upconversion nanoparticle (csUCNP) and gold nanoparticle (AuNP) for accurate detection of SARS‐CoV‐2 viral RNA is presented. In this biodetection assay, the Tm3+/Er3+ co‐doped csUCNP NaGdF4:Yb/Tm@NaYF4:Yb/Er acts as an energy donor and AuNP serves as an energy acceptor. The upconversion emission of Tm3+ and the design of the core‐shell structure led to a simultaneous surface plasmon effect of AuNP. The localized surface plasmon resonance (LSPR) arising from collective oscillations of free electrons significantly enhanced FRET efficiency between Er3+ and AuNP. The as‐prepared biosensor obtained a limit of detection (LOD) as low as 750 am, indicating that the integration of FRET and surface plasmon into one biodetection assay significantly boosted the sensitivity of the biosensor. In addition, samples extracted from clinical samples are also utilized to validate the effectiveness of the biosensor. Therefore, this innovative plasmon‐enhanced FRET biosensor based on Tm3+/Er3+ co‐doped csUCNP may pave the way for rapid and accurate biodetection applications.
Neurodevelopmental and other phenotypes recurrently associated with heterozygous BAZ2B loss‐of‐function variants
Authors: Sewani Soha , Azamian Mahshid S. , Mendelsohn Bryce A. , Mau‐Them Frederic Tran , Réda Manon , Nambot Sophie , Isidor Bertrand , van der Smagt Jasper J. , Shen Joseph J. , Shillington Amelle , White Lori , Elloumi Houda Zghal , Baker Peter R. , Svihovec Shayna , Brown Kathleen , Koopman‐Keemink Yvonne , Hoffer Mariette J. V. , Lakeman Inge M. M. , Brischoux‐Boucher Elise , Kinali Maria , Zhao Xiaonan , Lalani Seema R. , Scott Daryl A. 
Published: October 2023

AbstractThe bromodomain adjacent to zinc finger 2B (BAZ2B) gene encodes a chromatin remodeling protein that has been shown to perform a variety of regulatory functions. It has been proposed that loss of BAZ2B function is associated with neurodevelopmental phenotypes, and some recurrent structural birth defects and dysmorphic features have been documented among individuals carrying heterozygous loss‐of‐function BAZ2B variants. However, additional evidence is needed to confirm that these phenotypes are attributable to BAZ2B deficiency. Here, we report 10 unrelated individuals with heterozygous deletions, stop‐gain, frameshift, missense, splice junction, indel, and start‐loss variants affecting BAZ2B. These included a paternal intragenic deletion and a maternal frameshift variant that were inherited from mildly affected or asymptomatic parents. The analysis of molecular and clinical data from this cohort, and that of individuals previously reported, suggests that BAZ2B haploinsufficiency causes an autosomal dominant neurodevelopmental syndrome that is incompletely penetrant. The phenotypes most commonly seen in association with loss of BAZ2B function include developmental delay, intellectual disability, autism spectrum disorder, speech delay—with some affected individuals being non‐verbal—behavioral abnormalities, seizures, vision‐related issues, congenital heart defects, poor fetal growth, and an indistinct pattern of dysmorphic features in which epicanthal folds and small ears are particularly common.
Tweaking Photo CO2 Reduction by Altering Lewis Acidic Sites in Metalated‐Porous Organic Polymer for Adjustable H2/CO Ratio in Syngas Production
Authors: Paul Ratul , Das Risov , Das Nitumani , Chakraborty Subhajit , Pao Chih‐Wen , Thang Trinh Quang , Kalhara Gunasooriya G. T. Kasun , Mondal John , Peter Sebastian C. 
Published: October 2023

AbstractHerein, we have specifically designed two metalated porous organic polymers (Zn‐POP and Co‐POP) for syngas (CO+H2) production from gaseous CO2. The variable H2/CO ratio of syngas with the highest efficiency was produced in water medium (without an organic hole scavenger and photosensitizer) by utilizing the basic principle of Lewis acid/base chemistry. Also, we observed the formation of entirely different major products during photocatalytic CO2 reduction and water splitting with the help of the two catalysts, where CO (145.65 μmol g−1 h−1) and H2 (434.7 μmol g−1 h−1) production were preferentially obtained over Co‐POP & Zn‐POP, respectively. The higher electron density/better Lewis basic nature of Co‐POP was investigated further using XPS, XANES, and NH3‐TPD studies, which considerably improve CO2 activation capacity. Moreover, the structure–activity relationship was confirmed via in situ DRIFTS and DFT studies, which demonstrated the formation of COOH* intermediate along with the thermodynamic feasibility of CO2 reduction over Co‐POP while water splitting occurred preferentially over Zn‐POP.
Increasing Chemical Diversity of B2N2 Anthracene Derivatives by Introducing Continuous Multiple Boron‐Nitrogen Units
Authors: Jeong Seonghwa , Park Eunji , Kim Jiyeon , Park Seok Bae , Kim Sung Hoon , Choe Wonyoung , Kim Joonghan , Park Young S. 
Published: October 2023

AbstractIncreasing the chemical diversity of organic semiconductors is essential to develop efficient electronic devices. In particular, the replacement of carbon‐carbon (C−C) bonds with isoelectronic boron‐nitrogen (B−N) bonds allows precise modulation of the electronic properties of semiconductors without significant structural changes. Although some researchers have reported the preparation of B2N2 anthracene derivatives with two B−N bonds, no compounds with continuous multiple BN units have been prepared yet. Herein, we report the synthesis and characterization of a B2N2 anthracene derivative with a BNBN unit formed by converting the BOBN unit at the zigzag edge. Compared to the all‐carbon analogue 2‐phenylanthracene, BNBN anthracene exhibits significant variations in the C−C bond length and a larger highest occupied molecular orbital–lowest unoccupied molecular orbital energy gap. The experimentally determined bond lengths and electronic properties of BNBN anthracene are confirmed through theoretical calculations. The BOBN anthracene organic light‐emitting diode, used as a blue host, exhibits a low driving voltage. The findings of this study may facilitate the development of larger acenes with multiple BN units and potential applications in organic electronics.
Directly Knitted Hierarchical Porous Organometallic Polymer‐Based Self‐Supported Single‐Site Catalyst for CO2 Hydrogenation in Water
Authors: Mandal Tanmoy , Kumar Abhishek , Panda Jatin , Kumar Dutta Tapas , Choudhury Joyanta 
Published: October 2023

AbstractIn recent times, heterogenization of homogeneous molecular catalysts onto various porous solid support structures has attracted significant research focus as a method for combining the advantages of both homogeneous as well as heterogeneous catalysis. The design of highly efficient, structurally robust and reusable heterogenized single‐site catalysts for the CO2 hydrogenation reaction is a critical challenge that needs to be accomplished to implement a sustainable and practical CO2‐looped renewable energy cycle. This study demonstrated a heterogenized catalyst [Ir‐HCP‐(B/TPM)] containing a molecular Ir‐abnormal N‐heterocyclic carbene (Ir‐aNHC) catalyst self‐supported by hierarchical porous hyper‐crosslinked polymer (HCP), in catalytic hydrogenation of CO2 to inorganic formate (HCO2−) salt that is a prospective candidate for direct formate fuel cells (DFFC). By employing this unique and first approach of utilizing a directly knitted HCP‐based organometallic single‐site catalyst for CO2‐to‐HCO2− in aqueous medium, extremely high activity with a single‐run turnover number (TON) up to 50816 was achieved which is the highest so far considering all the heterogeneous catalysts for this reaction in water. Additionally, the catalyst featured excellent reusability furnishing a cumulative TON of 285400 in 10 cycles with just 1.6 % loss in activity per cycle. Overall, the new catalyst displayed attributes that are important for developing tangible catalysts for practical applications.
Tic‐related vs. non‐tic‐related obsessive compulsive disorder
Authors: Leckman James F. , Grice Dorothy E. , Barr Linda C. , de Vries Annelou L. C. , Martin Christine , Cohen Donald J. , McDougle Christopher J. , Goodman Wayne K. , Rasmussen Steven A. 
Published: October 2023

AbstractObsessive compulsive disorder (OCD) is a chronic condition characterized by an array of intrusive, upsetting thoughts and interfering, repetitive behaviors. Some forms of OCD may be etiologically related to Tourette's syndrome (TS). This cross‐sectional study examines a range of obsessive compulsive (OC) symptoms which have been hypothesized to distinguish tic‐related OCD from non‐tic‐related OCD. One hundred and seventy‐seven patients with a primary DSM‐IIIR diagnosis of OCD, aged 16 to 72, participated in the study. Patients with tic‐related OCD (n = 56) reported more OC symptoms, including more aggressive, religious, and sexual obsessions as well as checking, counting, ordering, touching, and hoarding compulsions than did patients with non‐tic‐related OCD (n = 121). Contrary to our expectation, these two groups of OCD patients did not differ with regard to the presence of “just right” phenomena or symptoms of psychasthenia. “just right” phenomena and symptoms of psychasthenia, however, were both found to be associated with the current severity of OC symptoms. Anxiety 1:208–215 (1994/1995). © 1995 Wley‐Liss, Inc.
Effect of cultivar and environment on chemical composition and geographical traceability of Spanish olive oils
Authors: Rey‐Giménez Raquel , Sánchez‐Gimeno Ana Cristina 
Published: October 2023

AbstractThis study aimed to investigate the influence of cultivar and environment on the chemical composition of Arbequina and Empeltre olive oils, and their contribution to geographical identification of olive oils from Aragon. A total of 260 olive oil samples from different cultivars (Arbequina, Empeltre, Royal de Calatayud, Alquezrana, and Royeta de Asque) from the three main oil‐producing areas of Aragon, located in northeast Spain, were selected. Fatty acid and sterol composition were analyzed in the course of three crop years (2017, 2018, and 2019). Cultivar was found the main factor influencing the variability of palmitic, palmitoleic, and linolenic fatty acid content, whereas geographic origin was the main contributor to variation in oleic and linoleic fatty acids in Arbequina and Empeltre olive oils. Cultivar also had a significant impact on sterol composition, although the effect of the production area also showed a significant effect on these oils. Crop year showed limited relevance, except for oleic and linoleic fatty acids. The interaction between the environment (e.g., crop year and geographical factors) and the cultivar (Arbequina and Empeltre) exerted a significant influence on oleic/linoleic (O/L) ratio and Δ7‐stigmastenol content, particularly in the southeast area of Aragon during the crop year with higher temperatures and drier conditions. Principal component analysis (PCA) and discriminant analysis (DA) confirmed the discriminative potential of the geographic production zone as a factor enabling the differentiation of olive oils from Aragon based on the major fatty acids and sterols.
Design of biocompatible polylactic acid–polyethylene glycol combination for manufacturing biodegradable staplers
Authors: Bakhtiary Negar , Bagheri Hamed , Salehnia Mojdeh 
Published: October 2023

AbstractFor the development of an effective and faster wound closure method, it is necessary to overcome current techniques' limitations. As compared to other methods, biodegradable staples provide superior mechanical properties, reduce scarring, and promote healing faster. Here, polylactic acid (PLA) and polyethylene glycol (PEG) composite staplers produced by solvent casting are presented as a suggestion for rapidly and easily closing wounds. The resulting samples were tested for mechanical properties, contact angle, and degradation properties over 21–90 days in the engineering phase. Selected formulations were moved to the biological phase for in vitro cytotoxicity by MTT assay and the in vivo biocompatibility assessment by transplantation of the samples into rats' back muscle. The tissue samples were collected and evaluated using hematoxylin and eosin and trichrome staining 1, 6, and 12 weeks after transplantation. When considering the maximum force tolerance range for PLA–PEG at around 20–58 N and comparing it with comparable commercial products, as well as their degradation rate and hydrophilicity, it was concluded that this combination maintains wound edges together. Cell viability tests demonstrated no cytotoxicity in the samples after 48 and 72 h. Morphological observations indicated some condensations of collagen fibers and no remarkable infiltration of inflammatory cells around the transplanted samples. In conclusion, the combination of PLA and PEG showed suitable mechanical properties, superior hydrophilicity, high biocompatibility and is suitable for medical staplers, and is innovative.