bluuebunny/crossref_metadata_2025_split huggingface dataset:
BRCC36 associates with FLT3‐ITD to regulate its protein stability and intracellular signaling in acute myeloid leukemia
Authors: Liu Jianwei , Isaji Tomoya , Komatsu Sachiko , Sun Yuhan , Xu Xing , Fukuda Tomohiko , Fujimura Tsutomu , Takahashi Shinichiro , Gu Jianguo 
Published: January 2024

AbstractFms‐like tyrosine kinase‐3 (FLT3) is a commonly mutated gene in acute myeloid leukemia (AML). The two most common mutations are the internal‐tandem duplication domain (ITD) mutation and the tyrosine kinase domain (TKD) mutation. FLT3‐ITD and FLT3‐TKD exhibit distinct protein stability, cellular localization, and intracellular signaling. To understand the underlying mechanisms, we performed proximity labeling with TurboID to identify proteins that regulate FLT3‐ITD or ‐TKD differently. We found that BRCA1/BRCA2‐containing complex subunit 36 (BRCC36), a specific K63‐linked polyubiquitin deubiquitinase, was exclusively associated with ITD, not the wild type of FLT3 and TKD. Knockdown of BRCC36 resulted in decreased signal transducers and activators of transcription 5 phosphorylation and cell proliferation in ITD cells. Consistently, treatment with thiolutin, an inhibitor of BRCC36, specifically suppressed cell proliferation and induced cell apoptosis in ITD cells. Thiolutin efficiently affected leukemia cell lines expressing FLT3‐ITD cell viability and exhibited mutual synergies with quizartinib, a standard clinical medicine for AML. Furthermore, mutation of the lysine at 609 of ITD led to significant suppression of K63 polyubiquitination and decreased its stability, suggesting that K609 is a critical site for K63 ubiquitination specifically recognized by BRCC36. These data indicate that BRCC36 is a specific regulator for FLT3‐ITD, which may shed light on developing a novel therapeutic approach for AML.
SIRT7 promotes Hippo/YAP activation and cancer cell proliferation in hepatocellular carcinoma via suppressing MST1
Authors: Gu Yiying , Ding Cong , Yu Tingzi , Liu Bohao , Tang Wenbin , Wang Zhiqiang , Tang Xiaohui , Liang Gaoshuang , Peng Jinying , Zhang Xiangwen , Li Zhuan 
Published: January 2024

AbstractAbnormal activation of the oncogene YAP in the Hippo pathway is a major feature in liver cancer and inactivation of MST1/2 has been shown to be responsible for the overactivation of YAP that led to tumorigenesis. However, mechanisms underlying MST1/2 dysregulation remain poorly understood. RNA‐seq analysis and genome (KEGG) pathway enrichment analysis were used to identify genes and pathways that were regulated by SIRT7. qRT‐PCR, ChIP, and luciferase assay were used to investigate transcriptional regulation. Mass spectrometry, co‐immunoprecipitation and immunoprecipitation were used to exam protein–protein interaction and post‐transcriptional modification. A xenograft mouse model was used to confirm the effect of SIRT7 and SIRT7 inhibitors on hepatocellular carcinoma (HCC) proliferation in vivo. We found that SIRT7 suppresses MST1 by both transcriptional regulation and post‐transcriptional modification, which in turn promotes YAP nuclear localization and transcriptional activation in liver cancer. Mechanistically, we revealed that SIRT7 suppresses MST1 transcription by binding to the MST1 promoter and inducing H3K18 deacetylation in its promoter region. In addition, SIRT7 directly binds to and deacetylates MST1, which primes acetylation‐dependent MST1 ubiquitination and protein degradation. In clinical samples, we confirmed a negative correlation between SIRT7 and MST1 protein levels, and high SIRT7 expression correlated with elevated YAP expression and nuclear localization. In addition, SIRT7 specific inhibitor 2800Z sufficiently inhibited HCC growth by disrupting the SIRT7/MST1/YAP axis. Our data thus revealed the previously undescribed function of SIRT7 in regulating the Hippo pathway in HCC and further proved that targeting SIRT7 might provide novel therapeutic options for the treatment of liver cancer.
Choroidal signal hypertransmission on optical coherence tomography imaging: Association with development of geographic atrophy in age‐related macular degeneration
Authors: Wu Zhichao , Terheyden Jan H. , Hodgson Lauren A. B. , Guymer Robyn H. 
Published: January 2024

AbstractBackgroundTo examine the association between large choroidal signal hypertransmission ≥250 μm (LHyperT) on optical coherence tomography (OCT) with the risk of developing geographic atrophy (GA) and compare this risk with those associated with nascent geographic atrophy (nGA).MethodsTwo hundred and eighty eyes from 140 participants with bilateral large drusen and without late age‐related macular degeneration (AMD) or nGA at baseline underwent OCT imaging and colour fundus photography (CFP) at 6‐monthly intervals up to 5 years. OCT scans were graded for the presence of LHyperT and nGA, and CFPs were graded for the presence of GA.ResultsThe five‐year incidence of LHyperT and nGA were 37% and 27% respectively (p = 0.003), and the two‐year probability of their progression to GA were 17% and 40%, respectively (p = 0.002). LHyperT and nGA explained 81% and 91% of the variance in the time to develop GA, respectively (p = 0.032), and they were both associated with a significantly higher rate of GA development compared to eyes without these lesions (adjusted hazard ratio = 110.8 and 183.2, respectively; p < 0.001 for both).ConclusionsLHyperT and nGA were both high‐risk features for GA development, but the latter showed a higher rate of GA progression and explained a significantly greater proportion of the variance in the time to develop GA. As such, nGA may be a more robust surrogate endpoint than LHyperT for the conventional clinical endpoint of CFP‐defined GA for intervention trials in the early stages of AMD.
Associative learning of visual cues in the fruit fly parasitoid Diachasmimorpha longicaudata: temporal dynamics and potential consequences on females' fitness
Authors: Nussenbaum Ana L. , Devescovi Francisco , Bachmann Guillermo E. , Pacelli Franco , Garbalena Micaela , Segura Diego F. 
Published: January 2024

AbstractDiachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae) is a generalist larval‐prepupal parasitoid of true fruit flies (Diptera: Tephritidae). During host‐seeking, females use visual information to locate the habitat of the host (fruit of various species). Previous studies have shown that females associate the colour of the host habitat with the availability of hosts through associative learning. In this study, we aimed to understand how this process is reinforced as the number of positive experiences increases and also how stable this association is, when the positive experiences cease. Additionally, under the hypothesis that associative learning has adaptive value, we evaluated the benefits of learning in two contexts: one in which host larvae are still associated with the learned colour and one where larvae are associated with a different colour. Repeated exposure to the unconditional stimulus for 1, 3, 5, and 7 consecutive training bouts influenced the preference response, as the percentage of females that preferred the stimulus increases with the number of training sessions. This preference seems to be reversible, as we found that the memory duration declines as time since the last training bout increased. The results obtained regarding the benefits of learning showed that females that learned a particular colour associated with the host, spent more time in that colour, made more oviposition attempts, and produced more offspring compared to females that learned a colour that is no longer associated with the host. This suggests that learning should not be fixed, and females would benefit from adjusting their behaviour to host availability. Such colour would allow females to find their host more efficiently, by associating cues that lead to host finding but also modifying their response when the cues are no longer associated with a reward, which seems a reasonable strategy for parasitoids of polyphagous hosts infesting ephemeral and changing habitats, such as fruit of different species.
Angular leaf spot of Caucasian alder incited by a pathovar of Xanthomonas arboricola
Authors: Abbasi Valeh , Rahimian Heshmatollah , Tajick‐Ghanbari Mohammad Ali , Barzegar Ali 
Published: January 2024

AbstractCaucasian alder (Alnus subcordata) is among the major constituents of the Hyrcanian forests extending all along the southern coast of the Caspian Sea in northern Iran. An angular leaf spot with a yellowish chlorotic halo has attained a widespread occurrence throughout the forest and suburban areas of the northern provinces including Guilan, Mazandaran and Golestan. Strains of a Xanthomonas sp. were consistently isolated from the symptomatic leaves of A. subcordata. Colonies of the isolates were yellow circular, convex and mucoid on nutrient agar containing glucose or sucrose. In multilocus sequence analysis using the genes gyrB, rpoD, dnaK, gltA, fyuA and gapA, the representative isolates occupied a clade shared by the known pathovars of Xanthomonas arboricola. The isolates were relatively heterogeneous phenotypically, but more so in their rep‐PCR fingerprints. Pathogenicity of several isolates was confirmed on seedlings of A. subcordata and Alnus rhombifolia, whereas Alnus cordata, Alnus glutinosa and Alnus crispa appeared not to be susceptible. Based on these characteristics, the isolates causing angular leaf spots of Caucasian alder appear to represent a novel pathovar of X. arboricola.
The origins of sedimentation inHusserl's phenomenology
Authors: Geniusas Saulius 
Published: January 2024

AbstractHusserl is the philosopher who transformed the geological metaphor of sedimentation into a philosophical concept. While tracing the development of Husserl's reflections on sedimentation, I argue that the distinctive feature of Husserl's approach lies in his preoccupation with the question concerning the origins of sedimentations. The paper demonstrates that in different frameworks of analysis, Husserl understood these origins in significantly different ways. In the works concerned with the phenomenology of time consciousness, Husserl searched for the origins of sedimentation in the field of subjective experience, and more precisely, in impressional consciousness. By contrast, in the later works concerned with history, he maintained that the origins of sedimentations lie in the field of historical past that stretches beyond the reach of individual experience. Building on the basis of these resources, I argue that the Husserlian concept of sedimentation has three distinct components of senses: static, genetic, and generative. In the static sense, sedimentations are modifications of retentions and necessary conditions of recollection. In the genetic sense, sedimentations are necessary for the formation of types, habits and moods, and as such, they shape present experiences. In the generative sense, sedimentations refer to what consciousness inherits from the historical tradition.
A systematic review of theories, models and frameworks used for youth engagement in health research
Authors: Sanchez Sherald , Thorburn Rachel , Rea Marika , Kaufman Pamela , Schwartz Robert , Selby Peter , Chaiton Michael 
Published: January 2024

AbstractBackgroundYouth engagement in research, wherein youth are involved in the research beyond mere participation as human subjects, is growing and becoming more popular as an approach to research. However, systematic and deliberate theory‐building has been limited. We conducted a systematic review to identify and synthesize theories, models and frameworks that have been applied in the engagement of youth in health research, including mental health.MethodsSix academic databases (MEDLINE, PsycINFO, Embase, PubMed, Scopus, CINAHL) and the grey literature were searched for relevant studies. Citation tracking was conducted through ancestry and descendancy searches. The final search was completed on 7 February 2023. Findings were summarized in a narrative synthesis informed by principles of hermeneutic analysis and interpretation. Reporting of results is in accordance with the PRISMA (Preferred Reporting Items for Systematic reviews and Meta‐Analyses) 2020 Statement.ResultsOf the 1156 records identified, 16 papers were included, from which we extracted named theories (n = 6), implicit theories (n = 5) and models and frameworks (n = 20) used for youth engagement in health research. We identified theories that were explicitly stated and surfaced theories that were more implicitly suggested. Models and frameworks were organized into four categories based on their principal features: power‐focused (n = 8), process‐focused (n = 7), impact‐focused (n = 3) and equity‐focused (n = 2). Few frameworks (n = 5) were empirically tested in health‐related research.ConclusionsThe state of theoretical development in youth engagement in research is still evolving. In this systematic review, we identified theories, models and frameworks used for youth engagement in health research. Findings from this systematic review offer a range of resources to those who seek to develop and strengthen youth engagement in their own research.Patient or Public ContributionYouth engaged as patients in the research were not involved in planning or conducting the systematic review. However, youth researchers in their early to mid‐20s led the planning, implementation and interpretation of the review. As part of subsequent work, we formed a youth advisory board to develop a youth‐led knowledge mobilization intended for an audience of youth with lived experience of being engaged as patients in research.
New insight into Ca2+‐permeable channel in plant immunity
Authors: Wang Wei , Cheng Hang‐Yuan , Zhou Jian‐Min 
Published: January 2024

ABSTRACTCalcium ions (Ca2+) are crucial intracellular second messengers in eukaryotic cells. Upon pathogen perception, plants generate a transient and rapid increase in cytoplasmic Ca2+ levels, which is subsequently decoded by Ca2+ sensors and effectors to activate downstream immune responses. The elevation of cytosolic Ca2+ is commonly attributed to Ca2+ influx mediated by plasma membrane‐localized Ca2+–permeable channels. However, the contribution of Ca2+ release triggered by intracellular Ca2+‐permeable channels in shaping Ca2+ signaling associated with plant immunity remains poorly understood. This review discusses recent advances in understanding the mechanism underlying the shaping of Ca2+ signatures upon the activation of immune receptors, with particular emphasis on the identification of intracellular immune receptors as non‐canonical Ca2+‐permeable channels. We also discuss the involvement of Ca2+ release from the endoplasmic reticulum in generating Ca2+ signaling during plant immunity.
Time‐varying associations between parental closeness, self‐esteem, and sexual behavior across adolescence and emerging adulthood
Authors: Jiang Linghua , Wang Xiafei , Cui Shuangyue , Vasilenko Sara A. 
Published: January 2024

AbstractThis study applied the time‐varying effect model (TVEM) to data from the National Longitudinal Study of Adolescent to Adult Health to explore how self‐esteem mediated age‐varying associations of closeness to mother and father and their child's sexual behavior through adolescence and emerging adulthood. Paternal closeness is associated with lesser odds of sexual behaviors for both female and male adolescents until age 20, whereas maternal closeness only predicts for female adolescents between ages 13 and 15. Self‐esteem mediated the association between mother closeness and multiple partners in male adolescents between ages 14.5 and 16.5. Fathers have an impact on adolescent sexual behavior across adolescence and emerging adulthood, while mothers' roles are more important for female adolescents in early adolescence.
An endophytic Trichoderma‐based wettable powder formulation for biocontrol of apple stem cankers
Authors: Ranjbar Zahra , Salehi Mina , Safaie Naser 
Published: January 2024

AbstractFungal stem cankers are common and destructive diseases in apple orchards. Cytospora cincta and Neoscytalidium dimidiatum are the apple canker causal agents in Iran. Formulated biocontrol agents of plant diseases are considered alternatives to synthetic pesticides in sustainable agriculture. The results showed that Trichoderma zelobreve T20 strongly inhibited the in vitro growth of C. cincta and N. dimidiatum. Wettable powder formulation (WPF) based on T. zelobreve T20 was developed to control apple canker caused by C. cincta and N. dimidiatum. The dual culture test showed that T20 inhibited C. cincta and N. dimidiatum growth by 55.19% and 44.07% respectively. T20 culture filtrate reduced the mycelial growth of C. cincta (72.22%) but showed no activity against N. dimidiatum. T20‐based WPF inhibited C. cincta (94.62%) and N. dimidiatum (14.71%) growth on detached branches of 2‐year‐old apple trees (golden delicious cultivar). Also, T20‐based WPF inhibited apple tree canker caused by C. cincta and N. dimidiatum by 82.8% and 60% respectively. The performance of T20‐based WPF on apple trees was significantly better than detached branches. The spore count of T20‐based WPF was about 71.54% of the original count after 4 months, marking its suitable shelf life. T20‐based WPF could be considered a promising control measure against apple canker pathogens.
Habitat loss and small‐scale fishery: A controversial issue
Authors: Silvestrini Chiara , Colletti Alberto , Di Franco Antonio , Colloca Francesco , Milisenda Giacomo , Zampardi Serena , Mangano Maria Cristina , Aglieri Giorgio , Ranù Marco , Liguori Gianluca , Danovaro Roberto , Foglini Federica , Grande Valentina , Fraschetti Simonetta 
Published: January 2024

AbstractFishery is one of the most impacting human activities and is responsible for habitat loss in marine systems. While the effects of large‐scale fisheries have been largely investigated, impacts of small‐scale fisheries (SSF) on seafloor integrity are more often assumed than quantitatively investigated. We carried out a literature review at global scale, resulting in 19 studies with quantitative data on sessile benthic bycatch and only one documenting habitat loss driven by SSF. We also conducted a fine‐scale assessment within a Mediterranean partially protected area (PPA). Results showed that 513 m2 of the Posidonia oceanica meadow are removed annually by local SSF within the PPA, considering bycatch, fishing effort, and shoot density. Knowledge on fishing effort and fine‐scale mapping is critical to assess habitat loss, suggesting the need for specific recommendations for eco‐sustainable local fisheries.
Global mesozooplankton communities show lower connectivity in deep oceanic layers
Authors: Canals Oriol , Corell Jon , Villarino Ernesto , Chust Guillem , Aylagas Eva , Mendibil Iñaki , Michell Craig T. , González‐Gordillo Juan Ignacio , Irigoien Xabier , Rodríguez‐Ezpeleta Naiara 
Published: January 2024

AbstractMesozooplankton is a key component of the ocean, regulating global processes such as the carbon pump, and ensuring energy transfer from lower to higher trophic levels. Yet, knowledge on mesozooplankton diversity, distribution and connectivity at global scale is still fragmented. To fill this gap, we applied DNA metabarcoding to mesozooplankton samples collected during the Malaspina‐2010 circumnavigation expedition across the Atlantic, Indian and Pacific oceans from the surface to bathypelagic depths. We highlight the still scarce knowledge on global mesozooplankton diversity and identify the Indian Ocean and the deep sea as the oceanic regions with the highest proportion of hidden diversity. We report no consistent alpha‐diversity patterns for mesozooplankton at a global scale, neither across vertical nor horizontal gradients. However, beta‐diversity analysis suggests horizontal and vertical structuring of mesozooplankton communities mostly attributed to turnover and reveals an increase in mesozooplankton beta‐diversity with depth, indicating reduced connectivity at deeper layers. Additionally, we identify a water mass type‐mediated structuring of mesozooplankton bathypelagic communities instead of an oceanic basin‐mediated as observed at upper layers. This suggests limited dispersal at deep ocean layers, most likely due to weaker currents and lower mixing of water mass types, thus reinforcing the importance of oceanic currents and barriers to dispersal in shaping global plankton communities.
The ABI4‐RGL2 module serves as a double agent to mediate the antagonistic crosstalk between ABA and GA signals
Authors: Xian Baoshan , Rehmani Muhammad Saad , Fan Yueni , Luo Xiaofeng , Zhang Ranran , Xu Jiahui , Wei Shaowei , Wang Lei , He Juan , Fu Aigen , Shu Kai 
Published: January 2024

Summary

Abscisic acid (ABA) and gibberellins (GA) antagonistically mediate several biological processes, including seed germination, but the molecular mechanisms underlying ABA/GA antagonism need further investigation, particularly any role mediated by a transcription factors module.

Here, we report that the DELLA protein RGL2, a repressor of GA signaling, specifically interacts with ABI4, an ABA signaling enhancer, to act as a transcription factor complex to mediate ABA/GA antagonism.

The rgl2, abi3, abi4 and abi5 mutants rescue the non‐germination phenotype of the ga1‐t. Further, we demonstrate that RGL2 specifically interacts with ABI4 to form a heterodimer. RGL2 and ABI4 stabilize one another, and GA increases the ABI4‐RGL2 module turnover, whereas ABA decreases it. At the transcriptional level, ABI4 enhances the RGL2 expression by directly binding to its promoter via the CCAC cis‐element, and RGL2 significantly upregulates the transcriptional activation ability of ABI4 toward its target genes, including ABI5 and RGL2. Abscisic acid promotes whereas GA inhibits the ability of ABI4‐RGL2 module to activate transcription, and ultimately ABA and GA antagonize each other. Genetic analysis demonstrated that both ABI4 and RGL2 are essential for the activity of this transcription factor module.

These results suggest that the ABI4‐RGL2 module mediates ABA/GA antagonism by functioning as a double agent.
Temporomandibular joint disorders and associated factors in a Turkish pediatric population
Authors: Torul Damla , Yılmaz Muhammed Furkan , Örnek Akdoğan Emine , Omezli Mehmet Melih 
Published: January 2024

AbstractObjectiveTo explore the presence and severity of temporomandibular disorders (TMD) signs and symptoms in a Turkish pediatric population, as well as their relationship to parafunctional behaviors, bruxism, malocclusion, anxiety, and sociodemographic traits.MethodsA cross‐sectional study was conducted with patients between the ages of 5–15. Demographic variables, TMD signs and symptoms, parafunctional habits, bruxism, and malocclusion were evaluated by clinical examination. Fonseca Anamnestic Index (FAI) was used to determine the presence and severity of TMD. State‐Trait Anxiety Scale for Children (STAI‐C) was used to evaluate the anxiety. Student t, Mann–Whitney U, chi‐square tests, and logistic regression were used for statistical analysis.ResultsOne hundred sixty‐two participants (85 girls, 77 boys) with a mean age of 10.03 ± 1.88 years were included. The frequency of the participants who reported signs/symptoms of TMD was 19.7% according to FAI scores. Logistic regression analysis with the associated factors showed a significant relationship between the presence of the signs/symptoms of TMD and parafunctional habits (OR 4.24, 95% CI 1.64–10.93), and the presence of signs/symptoms of anxiety (OR 1.09, 95% CI 1.02–1.16).ConclusionsChildren and adolescents who exhibit parafunctional behaviors and anxiety had a higher likelihood of having TMD signs/symptoms of varying degrees of severity.