bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rational design and performance prediction of organic photosensitizer based on TATA+ dye for hydrogen production by photocatalytic decomposition of water
Authors: Yu Yuening , Yang Zhenqing , Xia Yuhong , Lv Yuzhuo , Zhang Wansong , Lin Chundan , Shao Changjin 
Published: December 2023

In comparison to metal complexes, organic photosensitive dyes employed in photocatalytic hydrogen production exhibit promising developmental prospects. Utilizing the organic dye molecule TA+0 as the foundational structure, a series of innovative organic dyes, denoted as TA1-1 to TA2-6, were systematically designed. Employing first-principles calculations, we methodically explored the modifying effects of diverse electron-donating groups on the R1 and R2 positions to assess their application potential. Our findings reveal that, relative to the experimentally synthesized TATA+03, the TA2-6 molecule boasts a spatial structure conducive to intramolecular electron transfer, showcasing the most negative reduction potential (Ered = −2.11 eV) and the maximum reaction driving force (△G02 = −1.26 eV). This configuration enhances its compatibility with the reduction catalyst, thereby facilitating efficient hydrogen evolution. The TA2-6 dye demonstrates outstanding photophysical properties and a robust solar energy capture capacity. Its maximum molar extinction coefficient (ε) stands at 2.616 × 104 M−1·cm−1, representing a remarkable 292.8% improvement over TATA+03. In conclusion, this research underscores the promising potential of the TA2-6 dye as an innovative organic photosensitizer, positioning it as an efficacious component in homogeneous photocatalytic systems.
Elevated methane alters dissolved organic matter composition in the Arctic Ocean cold seeps
Authors: Sert Muhammed Fatih , Schweitzer Hannah D. , de Groot Tim R. , Kekäläinen Timo , Jänis Janne , Bernstein Hans C. , Ferré Bénédicte , Gründger Friederike , Kalenitchenko Dimitri , Niemann Helge 
Published: December 2023

Cold seeps release methane (CH4) from the seafloor to the water column, which fuels microbially mediated aerobic methane oxidation (MOx). Methane-oxidising bacteria (MOB) utilise excess methane, and the MOB biomass serves as a carbon source in the food web. Yet, it remains unclear if and how MOx modifies the composition of dissolved organic matter (DOM) in cold seeps. We investigated MOx rates, DOM compositions and the microbial community during ex-situ incubations of seawater collected from a cold seep site at Norskebanken (north of the Svalbard archipelago) in the Arctic Ocean. Samples were incubated with and without methane amendments. Samples amended with methane (∼1 µM final concentration) showed elevated rates of MOx in both seep and non-seep incubations. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) analyses showed that the number of DOM formulas (i.e., molecular diversity) increased by up to 39% in these incubations. In contrast, the number of formulas decreased by 20% in samples not amended with methane, both from non-seep and seep locations. DOM composition was thus altered towards a more diverse and heterogeneous composition along with elevated methanotrophic activity in methane-amended conditions. In addition to microbial DOM production, abating microbial diversity indicates that elevated DOM diversity was potentially related to grazing pressure on bacteria. The diversity of DOM constituents, therefore, likely increased with the variety of decaying cells contributing to DOM production. Furthermore, based on a principal coordinate analysis, we show that the final DOM composition of non-seep samples amended with methane became more resemblant to that of seep samples. This suggests that methane intrusions will affect water column DOM dynamics similarly, irrespective of the water column’s methane history.
Aesthetic surprises and considerations when researching marine science education with art
Authors: Hannigan Shelley M. , Freitas Cátia , Francis Prue 
Published: December 2023

IntroductionWhy was the study undertaken? What was the research question, the tested hypothesis or the purpose of the research? The research question is: What are the implications of disciplinary aesthetics when marine science meets art in educational research? Children in schools from Victoria, Australia were engaged in a series of marine science fieldtrips, workshops and lessons based on the Great Southern Reef, a temperate marine environment of Australia. They created drawings based on provocations, to depict their knowledge of marine species, before and after these education experiences.MethodsWhen, where, and how was the study done? What materials were used or who was included in the study groups (patients, etc.)? This paper shares the mixed methodology used by focusing on the qualitative methods used, that arose out of a need to understand the role of aesthetics in this research project. This paper documents the analysis of data that included children’s drawings and dialogue between researchers and children from interviews. We discuss insights into the role of aesthetics that were revealed in the visual and narrative data from perspectives of children’s learning and how the researchers were able to understand this. These findings are discussed considering the teaching intentions and procedures used, the importance of this multimodal approach to research that revealed aesthetics of science, visual art and language in education.ResultsWhat answer was found to the research question; what did the study find? Was the tested hypothesis true? The research reveals the important role drawing has when trying to understand the students’ varying degrees of understanding marine science education. Variables include: their prior experience with marine environments, students’ drawing abilities, stylistic elements (that can render an image ‘confident’ or ‘sketchy’), compositional devices and use of perspective that their drawings depict (looking at a pier from underwater or through snorkel goggles). It also includes interpretations and explanations of their drawings and other uses of language such as the use of written labels to reinforce or clarify parts of their drawings.DiscussionWhat might the answer imply and why does it matter? How does it fit in with what other researchers have found? What are the perspectives for future research? This research reveals the important role of multi-modal approaches in science learning and the significant and dependent role of visual art and words, for students to communicate their learnt content knowledge. It highlights the aesthetic experiences that must be taken into consideration when teaching, learning and when understanding what has been learnt.
Can environmental protection tax drive manufacturing carbon unlocking? Empirical evidence from China
Authors: Wen Limin , Sun Shufang 
Published: December 2023

IntroductionChina’s environmental protection tax policy has emerged as a pivotal force in steering the country towards reduced pollution and carbon emissions. This study focuses on evaluating the impact of the environmental protection tax on carbon unlocking within the manufacturing industry, offering crucial insights to address the dual challenges of industry development and carbon emission reduction.MethodsUtilizing Chinese provincial panel data spanning from 2004 to 2020, we gauge the carbon unlocking efficiency of the manufacturing industry using the super-efficiency slacks-based measure (SBM) model with undesired outputs. Additionally, we empirically examine the impact of the environmental protection tax policy on manufacturing carbon unlocking through the propensity score matching difference-in-differences (PSM-DID) model.ResultsOur findings indicate a significant enhancement in the carbon unlocking efficiency of the manufacturing industry due to the implementation of the environmental protection tax policy. Compared with the provinces (municipalities) that did not raise the environmental protection tax rate standard, the carbon unlocking efficiency of manufacturing in the provinces (municipalities) that raised the environmental protection tax rate standard increased by 11.6%, and the dynamic effect shows that the policy effect increases gradually over time. Further mechanism tests reveal that an environmental protection tax improves manufacturing carbon unlocking efficiency mainly by stimulating manufacturing firms to increase environmental protection investment and green technology innovation. Moreover, there is heterogeneity in the policy effect of the environmental protection tax, and its impact on manufacturing carbon unlocking is more significant in the central and western regions and regions with higher levels of legalization.DiscussionTherefore, we should adhere to the policy orientation of low-carbon development in manufacturing, accelerate green technological innovation in manufacturing, and coordinate the special advantages of each region according to local conditions to bring into play the role of an environmental protection tax in reducing carbon and improving efficiency.
Leveraging a gene signature associated with disulfidptosis identified by machine learning to forecast clinical outcomes, immunological heterogeneities, and potential therapeutic targets within lower-grade glioma
Authors: Zhou Yao , Cao Yudong , Liu Weidong , Wang Lei , Kuang Yirui , Zhou Yi , Chen Quan , Cheng Zeyu , Huang Haoxuan , Zhang Wenlong , Jiang Xingjun , Wang Binbin , Ren Caiping 
Published: December 2023

BackgroundDisulfidptosis, a newly defined type of programmed cell death, has emerged as a significant regulatory process in the development and advancement of malignant tumors, such as lower-grade glioma (LGG). Nevertheless, the precise biological mechanisms behind disulfidptosis in LGG are yet to be revealed, considering the limited research conducted in this field.MethodsWe obtained LGG data from the TCGA and CGGA databases and performed comprehensive weighted co-expression network analysis, single-sample gene set enrichment analysis, and transcriptome differential expression analyses. We discovered nine genes associated with disulfidptosis by employing machine learning methods like Cox regression, LASSO regression, and SVM-RFE. These were later used to build a predictive model for patients with LGG. To confirm the expression level, functional role, and impact on disulfidptosis of ABI3, the pivotal gene of the model, validation experiments were carried out in vitro.ResultsThe developed prognostic model successfully categorized LGG patients into two distinct risk groups: high and low. There was a noticeable difference in the time the groups survived, which was statistically significant. The model’s predictive accuracy was substantiated through two independent external validation cohorts. Additional evaluations of the immune microenvironment and the potential for immunotherapy indicated that this risk classification could function as a practical roadmap for LGG treatment using immune-based therapies. Cellular experiments demonstrated that suppressing the crucial ABI3 gene in the predictive model significantly reduced the migratory and invasive abilities of both SHG44 and U251 cell lines while also triggering cytoskeletal retraction and increased cell pseudopodia.ConclusionThe research suggests that the prognostic pattern relying on genes linked to disulfidptosis can provide valuable insights into the clinical outcomes, tumor characteristics, and immune alterations in patients with LGG. This could pave the way for early interventions and suggests that ABI3 might be a potential therapeutic target for disulfidptosis.
Appropriate intradeltoid muscle needle penetration depth in vaccine administration: an MRI study in Thailand
Authors: Laohawiriyakamol Teeranan , Boonsri Pattira , Phutphithak Khetnathee , Niyomkarn Thamonchanok , Klabklay Prapakorn , Chuaychoosakoon Chaiwat 
Published: December 2023

ObjectiveThe objective of this study was to evaluate the appropriate vaccination needle penetration depth into the deltoid muscle to avoid injection-site complications from an inappropriate injection depth and/or injection site in the Thai population.MethodsThis was a retrospective study using axial proton density-weighted images of MRI shoulders at the level of 2 fingerbreadths below the acromion process to measure the combined thickness of the skin, subcutaneous fat pad and deltoid muscle to evaluate the percentage of injections into the deltoid muscle with various needle penetration depths.ResultsThere were 509 MRI shoulder images of 222 males and 287 females (265 right shoulders and 244 left shoulders). The average body mass index and age were 24.54 ± 3.54 kg/m2 and 64.81 ± 10.20 years, respectively. Using a needle penetration depth of 12.7 mm (0.5 inches) achieved 100% of injections into the deltoid muscle.ConclusionWe recommend advancing the entire length of a 0.5-inch needle perpendicular to the skin at 2 fingerbreadths below the acromion process for adult intradeltoid vaccinations. This approach ensures optimal vaccine delivery and minimizes the risk of injection-related injuries.
Case report: hepatic arterial infusion chemotherapy combined with sintilimab and lenvatinib for conversion therapy of colorectal cancer liver metastases
Authors: Peng Kai , Li Yuhua , Su Hao , Lan Chenlu , Huang Zaida , Wei Yongguang , Liao Xiwen , Peng Minhao , Peng Tao , Zhu Guangzhi 
Published: December 2023

BackgroundLiver metastasis is one of the most common causes of death in patients with colorectal cancer. Therefore, improving the treatment effect of liver metastatic carcinoma of colorectal cancer is also one of the effective ways to improve the survival time of patients with colorectal cancer. The main treatment method for liver metastasis of colorectal cancer is preoperative neoadjuvant chemotherapy through intravenous administration. However, no one has reported a conversion therapy approach for the treatment of colorectal cancer liver metastases patients through arterial infusion chemotherapy combined with targeted agents and PD-1 monoclonal antibody. This case reports a conversion therapy method of liver metastases of colorectal cancer by hepatic arterial infusion chemotherapy (HAIC), sintilimab injection combined with lenvatinib to achieve radical resection of liver metastatic carcinoma after treatment.Case presentationThe patient was a 69-year-old man who had previously undergone laparoscopic left hemicolectomy for descending colorectal cancer and multiple interventional and surgical treatments for hepatocellular carcinoma. During this treatment, the patient underwent radiological and serological tests, and primary liver cancer was considered at the initial diagnosis stage. Therefore, this liver malignant tumor lesion was treated according to the primary liver cancer treatment protocol before surgical resection. Therefore, the patient received HAIC combined with sintilimab injection and lenvatinib. After three treatment cycles, radiological examination showed no obvious tumor activity, alpha-fetoprotein (AFP) decreased to normal, protein induced by vitamin K absence or antagonist II (PIVKA II) decreased significantly, and the curative effect was evaluated as complete remission. Subsequently, we performed surgical resection of this liver lesion. The pathological response of left lobe tumor was partial remission (PR). Most of the tumors were necrotic and the necrosis rate was greater than 95%. A small amount of live tumor tissue remains (&lt;5%). The pathological classification of this tumor was confirmed as moderately differentiated adenocarcinoma by immunohistochemical staining of multiple tumor indicators in the pathology department. No significant adverse drug events were observed in this patient during treatment.ConclusionHepatic arterial infusion chemotherapy combined with sintilimab injection and lenvatinib conversion therapy provides the opportunity for radical surgical resection of colorectal cancer liver metastases.
Mechanical and fatigue properties of graphene oxide concrete subjected to sulfate corrosion
Authors: Wang Xu , Zhang Guoliang , Liu Lang , Li Yongguang , Kong Hu , Zhang Cheng 
Published: December 2023

Concrete structures usually have to experience some unfavorable environmental actions during their service life, leading to the mechanical properties degradation, moreover, cyclic loadings such as traffic loads, wind loads may further accelerate structural damage, therefore, improving durability of concrete is of vital for structures servicing in severe environment. Graphene oxide (GO), as a new nano-reinforced material, has ultra-high mechanical properties and large specific surface area as a concrete reinforcing material. This study entailed an examination of the properties of Graphene Oxide Concrete (GOC) with varying levels of GO incorporation (0, 0.02, 0.05, 0.08 wt%). It also encompassed an analysis of the fatigue properties of GOC under different stress levels and varying sulfate wetting and drying cycles. Additionally, the investigation delved into the degradation mechanisms affecting Graphene Oxide Concrete (GOC) when subjected to sulfate erosion conditions. Furthermore, the study assessed the mass loss of specimens and their fatigue life under diverse environmental conditions. The results showed that appropriate GO incorporations could enhance concrete’s mechanical and fatigue properties after sulfate attack. In addition, scanning electron microscope (SEM) analysis showed that GO could adjust the aggregation state of cement hydration products and its own reaction with some cement hydration crystals to form strong covalent bonds, to improve and enhance microstructural denseness.
Subjective behavioral measures in myopic and pre-myopic children before and after the COVID lockdown
Authors: Alvarez-Peregrina Cristina , Ruiz-Pomeda Alicia , Martinez-Perez Clara , Prieto-Garrido Francisco Luis , Villa-Collar Cesar , Gonzalez-Perez Mariano , Gonzalez-Abad Ana , Sanchez-Tena Miguel Angel 
Published: December 2023

BackgroundThere are environmental factors that may contribute to the onset of myopia. This study aims to evaluate the children’s lifestyle changes before and after the COVID-19 lockdown and how they can influence their vision.MethodsThe same questionnaire was administered to children aged between 5 and 7 in Spain every year in September before (2017–2019) and after the COVID-19 lockdown (2020–2021). All the children also passed a vision exam consisting of the measurement of visual acuity (VA) and determination of objective and subjective refraction. Children were classified as myopes, pre-myopes, or hyperopes. The cut-off points to define the refractive error were established according to the value of the spherical equivalent (SE): hyperopia (SE &gt; +0,75D), myopia (SE ≤ −0,5D), or pre-myopia (−0.5D &lt; SE ≥ +0.75D). Data analysis is performed with the SPSS 27.0 software (SPSS Inc., Chicago, Illinois).ResultsIn the pre-COVID period, the pre-myopes were the ones who spent the longest time outdoors, and after the COVID lockdown, there were no differences between groups. There neither were any differences in the time spent doing near-work activities between the groups in both periods (p &gt; 0.05). Regarding the spherical equivalent, in the pre-COVID period, the mean value was 0.75 ± 2.09D and after the COVID lockdown, it was 0.47 ± 1.88D (p &lt; 0.001).ConclusionPre-myopes spent more time outdoors than myopes in the pre-COVID period, while myopes spent more time using digital devices. All these differences do not exist after the COVID lockdown, with a general increase in the time spent outdoors and a decrease in the use of digital devices. Further studies are needed to know if these lifestyle changes remain and how they influence the onset of myopia.
BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment options
Authors: Zhu Yan , Ding Zhechun , Wang Yini , Wu Qing , Chen Dongmei , Wang Luanhong , Li Yuancheng , Yao Yao , Huang Jiman , Li Yun , Wang Xiaojing , Lin Yanchun , Guan Tian , Zeng Haoyu , Li Congzhu 
Published: December 2023

IntroductionIn cancer treatment, every minute counts. Due to the unpredictable behavior of cancer cells caused by continuous mutations, each cancer patient has a unique situation and may or may not respond to a specific drug or treatment. The process of finding an effective therapy can be time-consuming, but cancer patients do not have the luxury of time for trial and error. Therefore, a novel technology to fast generate a patient relevant organoid for the therapies selecting is urgently needed.MethodsUtilizing the new organoid technology by specially dissolving the mesenchyme in tumor tissues acquired from cancer patients, we realized the work of creating patient-specific organoids (PSO) within one day.ResultsPSO properties reflect those of its respective original in vivo tumor tissue and can be utilized to perform various in vitro drug sensitivity tests to identify the most effective clinical treatment for patients. Additionally, PSO can aid in assessing the efficacy of immune cell therapies.DiscussionOrganoid technology has advanced significantly in recent years. However, current cancer organoid methods involve creating 3D tumor tissue from 2D cancer cells or cell clusters, primarily for cancer research purposes aimed at investigating related molecular and cellular mechanisms of tumor development. These methods are research-driven, not tailored towards clinical applications, and cannot provide personalized information for individual patients. PSO filled the gap of clinic-driven and time-saving method for the personalized therapies selecting to the cancer patients.