bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and Relative Binding Affinity to Human Steroid Receptors of Substituted 3‐Aryloxycoumarins.
Authors: Bakhchinian Robert , Terrier Francois , Kirkiacharian Serge , Resche‐Rigon Michele , Bouchoux Francoise , Cerede Evelyne 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of peri‐Anellated Heterocyclic Systems Based on 3‐Substituted 1‐Aryl‐4,6‐dinitro‐1H‐indazoles.
Authors: Starosotnikov A. M. , Lobach A. V. , Vinogradov V. M. , Shevelev S. A. 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Facile Method for Synthesis of Alkyl Phenyl Selenides. The Reaction of Diphenyl Diselenide with Oxygen‐Containing Compounds Using La/Me3SiCl/cat.I2/cat.CuI System.
Authors: Nishino Toshiki , Nishiyama Yutaka , Sonoda Noboru 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereospecific Palladium(0)‐Catalyzed Reduction of 2‐Cyclobutylidenepropyl Esters: A Versatile Preparation of Diastereomeric Monoterpenoids: (.+‐.)‐Fragranol (VII) and (.+‐.)‐Grandisol (VIII).
Authors: Bernard Angela M. , Frongia Angelo , Secci Francesco , Delogu Giovanna , Ollivier Jean , Piras Pier P. , Salauen Jacques 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of Unsaturated, Fused Bicyclic Proline Analogues Through Amino Alkylation of Cyclic Bis(allylsulfoximine)titanium Complexes and Migratory Cyclization of δ‐Amino Alkenyl Aminosulfoxonium Salts.
Authors: Koep Stefan , Gais Hans‐Joachim , Raabe Gerhard 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Novel Entry to C‐Glycals via Diethylzinc‐Mediated Umpolung of π‐Allyl Palladium Derived from 1‐exo‐Methylene 2,3‐Anhydrofuranoses.
Authors: Gomez Ana M. , Barrio Aitor , Pedregosa Ana , Valverde Serafin , Lopez J. Cristobal 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel Short Oligonucleotide Conjugates as Inhibitors of Human Telomerase
Authors: Pongracz Krisztina , Li Shihong , Herbert Brittney‐Shea , Pruzan Ronald , Wunder Ellen , Chin Allison , Piatyszek Mieczyslaw , Shay Jerry , Gryaznov Sergei M. 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Influence of the Length of the Phosphate Chain in mRNA 5′ Cap Analogues on Their Interaction with Eukaryotic Initiation Factor 4E
Authors: Zuberek Joanna , Jemielity Jacek , Niedzwiecka Anna , Stepinski Janusz , Wyslouch‐Cieszynska Aleksandra , Stolarski Ryszard , Darzynkiewicz Edward 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 2‐Fluoronoraristeromycin and Its Inhibitory Activity Against Plasmodium falciparum S‐Adenosyl‐L‐homocysteine Hydrolase.
Authors: Kitade Yukio , Kojima Hiroharu , Zulfiqur Fazila , Kim Hye‐Sook , Wataya Yusuke 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Oncidinol — A Novel Diacylglycerol from Ornithophora radicans Barb. Rodr. (Orchidaceae) Floral Oil.
Authors: Reis Mariza Gomes , de Faria Aparecida Donisete , do Amaral Maria do Carmo Estanislau , Marsaioli Anita Jocelyne 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Formal [3 + 3] Cycloaddition Approach to Chromenes and Chromanes. Concise Total Syntheses of (.+‐.)‐Rhododaurichromanic Acids A and B and Methyl (.+‐.)‐Daurichromenic Ester.
Authors: Kurdyumov Aleksey V. , Hsung Richard P. , Ihlen Kirsten , Wang Jiashi 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Enantiospecific Formal Total Syntheses of (‐)‐Salicylihalamides A and B from D‐Glucose and L‐Rhamnose.
Authors: Yang Kyoung Lang , Haack Torsten , Blackman Burchelle , Diederich Wibke E. , Roy Subho , Pusuluri Srinivas , Georg Gunda I. 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Manganese‐Containing Silica‐Based Microporous Molecular Sieve MnS‐1: Synthesis and Characterization.
Authors: Tusar Natasa Novak , Logar Natasa Zabukovec , Arcon Iztok , Thibault‐Starzyk Frederic , Ristic Alenka , Rajic Nevenka , Kaucic Venceslav 
Published: July 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Localization and loss‐of‐function implicates ciliary proteins in early, cytoplasmic roles in left‐right asymmetry
Authors: Qiu Dayong , Cheng Shing‐Ming , Wozniak Laryssa , McSweeney Megan , Perrone Emily , Levin Michael 
Published: July 2005

AbstractLeft‐right asymmetry is a crucial feature of the vertebrate body plan. While much molecular detail of this patterning pathway has been uncovered, the embryonic mechanisms of the initiation of asymmetry, and their evolutionary conservation among species, are still not understood. A popular recent model based on data from mouse embryos suggests extracellular movement of determinants by ciliary motion at the gastrulating node as the initial step. An alternative model, driven by findings in the frog and chick embryo, focuses instead on cytoplasmic roles of motor proteins. To begin to test the latter hypothesis, we analyzed the very early embryonic localization of ciliary targets implicated in mouse LR asymmetry. Immunohistochemistry was performed on frog and chick embryos using antibodies that have (KIF3B, Polaris, Polycystin‐2, acetylated α‐tubulin) or have not (LRD, INV, detyrosinated α‐tubulin) been shown to detect in frog embryos only the target that they detect in mammalian tissue. Immunohistochemistry revealed localization signals for all targets in the cytoplasm of cleavage‐stage Xenopus embryos, and in the base of the primitive streak in chick embryos at streak initiation. Importantly, several left‐right asymmetries were detected in both species, and the localization signals were dependent on microtubule and actin cytoskeletal organization. Moreover, loss‐of‐function experiments implicated very early intracellular microtubule‐dependent motor protein function as an obligate aspect of oriented LR asymmetry in Xenopus embryos. These data are consistent with cytoplasmic roles for motor proteins in patterning the left‐right axis that do not involve ciliary motion. Developmental Dynamics 234:176–189, 2005. © 2005 Wiley‐Liss, Inc.
Multiple pressure variables methods for fluid flow at all Mach numbers
Authors: Park J. H. , Munz C.‐D. 
Published: July 2005

AbstractIn this paper we present a method for the simulation of incompressible as well as compressible unsteady flows. At first we discuss three different forms, i.e. a primitive‐, conservative‐ and a semi‐conservative form of the governing equations. We use a semi‐implicit time integration in such a fashion that the stability is guaranteed independently of the speed of sound and the resulting method is independent of the Mach number range. Moreover, with the application of the so‐called multiple pressure variables (MPV) approach the difficulties with the pressure term can be circumvented as in the incompressible limit the hydrodynamic pressure decouples from the equation of state. Increasing approximation errors in the low Mach number regime are avoided. As a result, the proposed algorithm can also simulate incompressible flows as limit for zero Mach number. Copyright © 2005 John Wiley & Sons, Ltd.
Recruitment of Total Phytoplankton, Chlorophytes and Cyanobacteria from Lake Sediments Recorded by Photosynthetic Pigments in a Large, Shallow Lake (Lake Taihu, China)
Authors: Cao Huan‐Sheng , Kong Fan‐Xiang , Tan Jian‐Kang , Zhang Xiao‐Feng , Tao Yi , Yang Zhou 
Published: July 2005

AbstractRecruitment of total phytoplankton, chlorophytes and cyanobacteria from lake sediments to the water column was studied using photosynthetic pigments at one site (1.5 m) in Lake Taihu, a large shallow lake in China. Samples were taken weekly from the migration traps installed on the bottom from March to May 2004. Abundance of total phytoplankton, chlorophytes and cyanobacteria were represented by Chlorophyll (Chl) a, b, and phycocyanin (PC), respectively. Over the three months, total phytoplankton, chlorophytes, and cyanobacteria corresponding to 48.9%, 68.9% and 316.2% of their initial concentrations in surface sediments were recruited in Lake Taihu. However, compared with their increase in pelagic abundance over the same period, the recruitment accounted for a rather small inoculum. Accompanying the recruitment, total phytoplankton and chlorophytes declined and cyanobacteria increased in the upper 0–2 cm sediments; colonies of Microcystis aeruginosa in the water column enlarged from small size with several cells to large colonies with hundreds of cells. Thus, overwintering and subsequent growth renewal of pelagic phytoplankton merits further study and comparison with benthic survival and recruitment. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry
Authors: Okuno Shoji , Wada Yoshinao 
Published: July 2005

AbstractThe applicability of the matrix‐free laser desorption/ionization on silicon mass spectrometry (DIOS‐MS) to measuring serum drug levels was examined by analyzing serum salicylic acid. The optimized and simple solid‐phase extraction (SPE) allowed good recovery, 88.9 ± 5.8%, for 1.4 mM (200 mg/L) of salicylic acid in serum. The negative ion MS allowed measurements of deprotonated molecules without interference from other signals. Using a deuterium‐labeled internal standard, good linearity was obtained in the 0.14 to 4.2 mM (20–600 mg/L) range, which was sufficient for monitoring the therapeutic anti‐inflammatory dose. SPE followed by DIOS‐MS is anticipated to be a method of measuring drug levels in blood and may allow high throughput analysis. Copyright © 2005 John Wiley & Sons, Ltd.
Preparation of Functionalized Core‐Shell Alumina/Polystyrene Composite Nanoparticles, 1
Authors: Zeng Zhong , Yu Jian , Guo Zhao‐Xia 
Published: July 2005

AbstractSummary: A convenient method for the preparation of well‐defined core‐shell alumina/polystyrene composite nanoparticles (50 nm) through emulsion polymerization from needle‐shaped alumina is reported. The pretreatment of alumina with 3‐methacryloxypropyltrimethoxysilane (MPTMS) is essential for successful encapsulation and for creating strong interfacial interaction between alumina and polystyrene (PS). High yield and binding efficiency (both higher than 90%) are achieved by a proper selection of the reaction conditions. As revealed by transmission electron microscopy (TEM) and atomic force microscopy (AFM), an ideal core‐shell structure is obtained with one alumina needle as the core and PS as the shell when the amount of alumina is appropriate. However, when more alumina is used, the PS latexes contain an agglomerate of a few alumina needles arranging in a parallel fashion without any precipitation of alumina. The analysis of the grafted and free PS has shown that the grafted PS has bigger molecular weight (MW) and larger molecular weight distribution (MWD) presumably due to some condensation between the methoxyl groups of the silane moiety. The polymerization kinetics and the encapsulation mechanism have also been discussed.Schematic representation of the encapsulation procedure.magnified imageSchematic representation of the encapsulation procedure.
gem‐Dimethyl Effects in the Thermal Behavior of Polyethylene
Authors: Schwendeman John E. , Wagener Kenneth B. 
Published: July 2005

AbstractSummary: Polyethylene can be rendered completely amorphous via the insertion of regularly spaced gem‐dimethyl defects along its backbone, where frequency of insertion is the deciding factor in achieving the totally amorphous state. Accurate placement of defects along polyethylene's backbone is achieved with step polymerization metathesis chemistry (the ADMET reaction) rather than using chain polymerization techniques. These gem‐dimethyl polyethylene macromolecules, when compared with other ADMET ethylene‐based model materials, demonstrate that steric bulk, frequency, and distribution of the defect along the polymer backbone are more important than stereoregularity (tacticity) when the defects are spaced nine carbon atoms or more apart. Comparisons are made between gem‐dimethyl “defects” and methyl “defects” in a polyethylene backbone.Synthetic scheme for the ADMET‐made gem‐dimethyl model polyethylenes (14a–c).magnified imageSynthetic scheme for the ADMET‐made gem‐dimethyl model polyethylenes (14a–c).
Characteristic Sliding Frictional Behavior on the Surface of Nanocomposite Hydrogels Consisting of Organic‐Inorganic Network Structure
Authors: Haraguchi Kazutoshi , Takada Tetsuo 
Published: July 2005

AbstractSummary: Sliding frictional behavior on the surface of novel nanocomposite hydrogels (NC gels), composed of poly(N‐isopropylacrylamide) and exfoliated clay, was investigated and compared with that of conventional chemically‐crosslinked hydrogels (OR gels). Since OR gels are so weak, the sliding test, in general, could not be carried out on their surfaces except when wet. On the contrary, NC gels can withstand sliding friction under high loading and it was found that the frictional forces are sensitive to the environmental surroundings (wet or in‐air), the gel composition (water, clay contents), and loading. In air, NC gels exhibit a characteristic force profile with a maximum static frictional force (max‐SFF) and subsequent constant dynamic frictional force. In contrast, NC gels exhibit very low frictional forces under wet conditions. The change in frictional force with surroundings was very distinctive for NC gels, particularly for those with low clay contents. Max‐SFF for NC1 gel decreased more than 100‐fold by changing the environment from in‐air to wet. The characteristic sliding frictional behaviors are explained on the basis of a unique organic/inorganic network structure of NC gels.The model of sliding process on NC gel and effects of clay content of NC gels on the sliding frictional behavior.magnified imageThe model of sliding process on NC gel and effects of clay content of NC gels on the sliding frictional behavior.
Sufficient conditions for optimal control problems with mixed constraints
Authors: Galewska E. , Nowakowski A. 
Published: July 2005

AbstractIn this paper we derive sufficient conditions for optimal control problems with mixed control and state constraints by applying a dual approach to the dynamic programming. These conditions guarantee that a relative minimum is achieved. We seek an optimal pair in the class of those admissible pairs for which graphs of trajectories belong to a set defined by a function satisfying a dual equation of dynamic programming. This set usually differs from the classical neighbourhood of an optimal trajectory. We prove a verification theorem and introduce an optimal dual feedback control. Copyright © 2005 John Wiley & Sons, Ltd.
Comparison of alternative analytical techniques for the characterisation of the human serum proteome in HUPO Plasma Proteome Project
Authors: Li Xiaohai , Gong Yan , Wang Ying , Wu Songfeng , Cai Yun , He Ping , Lu Zhuang , Ying Wantao , Zhang Yangjun , Jiao Liyan , He Hongzhi , Zhang Zisen , He Fuchu , Zhao Xiaohang , Qian Xiaohong 
Published: July 2005

AbstractBased on the same HUPO reference specimen (C1‐serum) with the six proteins of highest abundance depleted by immunoaffinity chromatography, we have compared five proteomics approaches, which were (1) intact protein fractionation by anion‐exchange chromatography followed by 2‐DE‐MALDI‐TOF‐MS/MS for protein identification (2‐DE strategy); (2) intact protein fractionation by 2‐D HPLC followed by tryptic digestion of each fraction and microcapillary RP‐HPLC/microESI‐MS/MS identification (protein 2‐D HPLC fractionation strategy); (3) protein digestion followed by automated online microcapillary 2‐D HPLC (strong cation‐exchange chromatography (SCX)‐RPC) with IT microESI‐MS/MS; (online shotgun strategy); (4) same as (3) with the SCX step performed offline (offline shotgun strategy) and (5) same as (4) with the SCX fractions reanalysed by optimised nanoRP‐HPLC‐nanoESI‐MS/MS (offline shotgun‐nanospray strategy). All five approaches yielded complementary sets of protein identifications. The total number of unique proteins identified by each of these five approaches was (1) 78, (2) 179, (3) 131, (4) 224 and (5) 330 respectively. In all, 560 unique proteins were identified. One hundred and sixty‐five proteins were identified through two or more peptides, which could be considered a high‐confidence identification. Only 37 proteins were identified by all five approaches. The 2‐DE approach yielded more information on the pI‐altered isoforms of some serum proteins and the relative abundance of identified proteins. The protein prefractionation strategy slightly improved the capacity to detect proteins of lower abundance. Optimising the separation at the peptide level and improving the detection sensitivity of ESI‐MS/MS were more effective than fractionation of intact proteins in increasing the total number of proteins identified. Overall, electrophoresis and chromatography, coupled respectively with MALDI‐TOF/TOF‐MS and ESI‐MS/MS, identified complementary sets of serum proteins.
Depletion of multiple high‐abundance proteins improves protein profiling capacities of human serum and plasma
Authors: Echan Lynn A. , Tang Hsin‐Yao , Ali‐Khan Nadeem , Lee KiBeom , Speicher David W. 
Published: July 2005

AbstractSystematic detection of low‐abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently commercialized HPLC column containing antibodies to six of the most abundant blood proteins (“Top‐6 depletion”) with either older Cibacron blue/Protein A or G depletion methods or no depletion. In addition, a prototype spin column version of the HPLC column and an alternative prototype two antibody spin column were evaluated. The HPLC polyclonal antibody column and its spin column version are very promising methods for substantially simplifying human serum or plasma samples. These columns show the lowest nonspecific binding of the depletion methods tested. In contrast other affinity methods, particularly dye‐based resins, yielded many proteins in the bound fractions in addition to the targeted proteins. Depletion of six abundant proteins removed about 85% of the total protein from human serum or plasma, and this enabled 10‐ to 20‐fold higher amounts of depleted serum or plasma samples to be applied to 2‐D gels or alternative protein profiling methods such as protein array pixelation. However, the number of new spots detected on 2‐D gels was modest, and most newly visualized spots were minor forms of relatively abundant proteins. The inability to detect low‐abundance proteins near expected 2‐D staining limits was probably due to both the highly heterogeneous nature of most plasma or serum proteins and masking of many low‐abundance proteins by the next series of most abundant proteins. Hence, non2‐D methods such as protein array pixelation are more promising strategies for detecting lower abundance proteins after depleting the six abundant proteins.
Cardiovascular‐related proteins identified in human plasma by the HUPO Plasma Proteome Project Pilot Phase
Authors: Berhane Beniam T. , Zong Chenggong , Liem David A. , Huang Aaron , Le Steven , Edmondson Ricky D. , Jones Richard C. , Qiao Xin , Whitelegge Julian P. , Ping Peipei , Vondriska Thomas M. 
Published: July 2005

AbstractProteomic profiling of accessible bodily fluids, such as plasma, has the potential to accelerate biomarker/biosignature development for human diseases. The HUPO Plasma Proteome Project pilot phase examined human plasma with distinct proteomic approaches across multiple laboratories worldwide. Through this effort, we confidently identified 3020 proteins, each requiring a minimum of two high‐scoring MS/MS spectra. A critical step subsequent to protein identification is functional annotation, in particular with regard to organ systems and disease. Performing exhaustive literature searches, we have manually annotated a subset of these 3020 proteins that have cardiovascular‐related functions on the basis of an existing body of published information. These cardiovascular‐related proteins can be organized into eight groups: markers of inflammation and/or cardiovascular disease, vascular and coagulation, signaling, growth and differentiation, cytoskeletal, transcription factors, channels/receptors and heart failure and remodeling. In addition, analysis of the peptide per protein ratio for MS/MS identification reveals group‐specific trends. These findings serve as a resource to interrogate the functions of plasma proteins, and moreover, the list of cardiovascular‐related proteins in plasma constitutes a baseline proteomic blueprint for the future development of biosignatures for diseases such as myocardial ischemia and atherosclerosis.
Analysis of Human Proteome Organization Plasma Proteome Project (HUPO PPP) reference specimens using surface enhanced laser desorption/ionization‐time of flight (SELDI‐TOF) mass spectrometry: Multi‐institution correlation of spectra and identification of biomarkers
Authors: Rai Alex J. , Stemmer Paul M. , Zhang Zhen , Adam Bao‐ling , Morgan William T. , Caffrey Rebecca E. , Podust Vladimir N. , Patel Manisha , Lim Lih‐yin , Shipulina Natalia V. , Chan Daniel W. , Semmes O. John , Leung Hon‐chiu Eastwood 
Published: July 2005

AbstractWe report on a multicenter analysis of HUPO reference specimens using SELDI‐TOF MS. Eight sites submitted data obtained from serum and plasma reference specimen analysis. Spectra from five sites passed preliminary quality assurance tests and were subjected to further analysis. Intralaboratory CVs varied from 15 to 43%. A correlation coefficient matrix generated using data from these five sites demonstrated high level of correlation, with values >0.7 on 37 of 42 spectra. More than 50 peaks were differentially present among the various sample types, as observed on three chip surfaces. Additionally, peaks at ∼9200 and ∼15 950 m/z were present only in select reference specimens. Chromatographic fractionation using anion‐exchange, membrane cutoff, and reverse phase chromatography, was employed for protein purification of the ∼9200 m/z peak. It was identified as the haptoglobin alpha subunit after peptide mass fingerprinting and high‐resolution MS/MS analysis. The differential expression of this protein was confirmed by Western blot analysis. These pilot studies demonstrate the potential of the SELDI platform for reproducible and consistent analysis of serum/plasma across multiple sites and also for targeted biomarker discovery and protein identification. This approach could be exploited for population‐based studies in all phases of the HUPO PPP.
Human Plasma PeptideAtlas
Authors: Deutsch Eric W. , Eng Jimmy K. , Zhang Hui , King Nichole L. , Nesvizhskii Alexey I. , Lin Biaoyang , Lee Hookeun , Yi Eugene C. , Ossola Reto , Aebersold Ruedi 
Published: July 2005

AbstractPeptide identifications of high probability from 28 LC‐MS/MS human serum and plasma experiments from eight different laboratories, carried out in the context of the HUPO Plasma Proteome Project, were combined and mapped to the EnsEMBL human genome. The 6929 distinct observed peptides were mapped to approximately 960 different proteins. The resulting compendium of peptides and their associated samples, proteins, and genes is made publicly available as a reference for future research on human plasma.