bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between healthy lifestyle factors and health-related quality of life among Chinese adolescents: the moderating role of gender
Authors: Xiang Hongyu , Feng Xiuqiong , Lin Li , Luo Shengyu , Liu Xinxia , Chen Dezhong , Qin Kang , Guo Xun , Chen Weiqing , Guo Vivian Yawei 
Published: October 2023

Abstract
Background
To examine the associations of the independent and combined healthy lifestyle factors with health-related quality of life (HRQOL) in adolescents, and to test the moderating role of gender.

Methods
This cross-sectional study included 5125 adolescents aged between 11 and 20 years. They provided self-reported data on six healthy lifestyle factors, including never smoking, never drinking, good sleep quality, sufficient sleep duration, appropriate Internet use, and adequate physical activity. Adolescents’ HRQOL was evaluated using the Pediatric Quality of Life Inventory version 4.0. Linear regression models were conducted to explore the association of individual and combined healthy lifestyle factors with adolescents’ HRQOL. We further performed stratified analyses and likelihood ratio test to explore the moderating role of gender in these associations.

Results
Of the included adolescents, the proportions with 0–2, 3, 4, and 5–6 healthy lifestyle factors were 13.6%, 26.4%, 44.3%, and 15.7%, respectively. Compared to adolescents with composite healthy lifestyle scores of 0–2, those with scores of 3, 4, or 5–6 had significantly higher HRQOL scores across all dimensions, summary scales, and total scale in both unadjusted and adjusted models. Specifically, adolescents with 5–6 healthy lifestyle factors had a total scale score that was 19.03 (95%CI: 17.76 to 20.30) points higher than their counterparts who only had 0–2 healthy lifestyle factors. Significant dose-response patterns were also observed in aforementioned associations. Gender was a significant moderator in the associations between composite healthy lifestyle groups and HRQOL scores, except for the social functioning dimension.

Conclusions
Our results confirmed that combined healthy lifestyle factors were associated with improved HRQOL among adolescents, with a stronger association observed in girls. These findings underscore the necessity for education and healthcare authorities to design health-promoting strategies that encourage multiple healthy lifestyle factors in adolescents, with the objective of enhancing their overall health outcomes.
Multiphase and multiparameter MRI-based radiomics for prediction of tumor response to neoadjuvant therapy in locally advanced rectal cancer
Authors: Huang Hongyan , Han Lujun , Guo Jianbo , Zhang Yanyu , Lin Shiwei , Chen Shengli , Lin Xiaoshan , Cheng Caixue , Guo Zheng , Qiu Yingwei 
Published: October 2023

AbstractBackgroundTo develop and validate radiomics models for prediction of tumor response to neoadjuvant therapy (NAT) in patients with locally advanced rectal cancer (LARC) using both pre-NAT and post-NAT multiparameter magnetic resonance imaging (mpMRI).MethodsIn this multicenter study, a total of 563 patients were included from two independent centers. 453 patients from center 1 were split into training and testing cohorts, the remaining 110 from center 2 served as an external validation cohort. Pre-NAT and post-NAT mpMRI was collected for feature extraction. The radiomics models were constructed using machine learning from a training cohort. The accuracy of the models was verified in a testing cohort and an independent external validation cohort. Model performance was evaluated using area under the curve (AUC), sensitivity, specificity, positive predictive value, and negative predictive value.ResultsThe model constructed with pre-NAT mpMRI had favorable accuracy for prediction of non-response to NAT in the training cohort (AUC = 0.84), testing cohort (AUC = 0.81), and external validation cohort (AUC = 0.79). The model constructed with both pre-NAT and post-NAT mpMRI had powerful diagnostic value for pathologic complete response in the training cohort (AUC = 0.86), testing cohort (AUC = 0.87), and external validation cohort (AUC = 0.87).ConclusionsModels constructed with multiphase and multiparameter MRI were able to predict tumor response to NAT with high accuracy and robustness, which may assist in individualized management of LARC.
In-organoid single-cell CRISPR screening reveals determinants of hepatocyte differentiation and maturation
Authors: Liang Junbo , Wei Jinsong , Cao Jun , Qian Jun , Gao Ran , Li Xiaoyu , Wang Dingding , Gu Yani , Dong Lei , Yu Jia , Zhao Bing , Wang Xiaoyue 
Published: October 2023

Abstract
Background
Harnessing hepatocytes for basic research and regenerative medicine demands a complete understanding of the genetic determinants underlying hepatocyte differentiation and maturation. Single-cell CRISPR screens in organoids could link genetic perturbations with parallel transcriptomic readout in single cells, providing a powerful method to delineate roles of cell fate regulators. However, a big challenge for identifying key regulators during data analysis is the low expression levels of transcription factors (TFs), which are difficult to accurately estimate due to noise and dropouts in single-cell sequencing. Also, it is often the changes in TF activities in the transcriptional cascade rather than the expression levels of TFs that are relevant to the cell fate transition.

Results
Here, we develop Organoid-based Single-cell CRISPR screening Analyzed with Regulons (OSCAR), a framework using regulon activities as readouts to dissect gene knockout effects in organoids. In adult-stem-cell-derived liver organoids, we map transcriptomes in 80,576 cells upon 246 perturbations associated with transcriptional regulation of hepatocyte formation. Using OSCAR, we identify known and novel positive and negative regulators, among which Fos and Ubr5 are the top-ranked ones. Further single-gene loss-of-function assays demonstrate that Fos depletion in mouse and human liver organoids promote hepatocyte differentiation by specific upregulation of liver metabolic genes and pathways, and conditional knockout of Ubr5 in mouse liver delays hepatocyte maturation.

Conclusions
Altogether, we provide a framework to explore lineage specifiers in a rapid and systematic manner, and identify hepatocyte determinators with potential clinical applications.
Glucose fluctuations aggravate myocardial fibrosis via activating the CaMKII/Stat3 signaling in type 2 diabtetes
Authors: Zhang Lei , Liu Huan-Huan , Yang Fan , Zhang Zhi-Yuan , Zhang Zhen-Ye , Zhao Xiao-Xi , Qian Ling-Ling , Dang Shi-Peng , Wang Ru-Xing 
Published: October 2023

Abstract
Background
Glucose fluctuations (GF) are a risk factor for cardiovascular complications associated with type 2 diabetes. However, there is a lack of adequate research on the effect of GF on myocardial fibrosis and the underlying mechanisms in type 2 diabetes. This study aimed to investigate the impact of glucose fluctuations on myocardial fibrosis and explore the potential mechanisms in type 2 diabetes.

Methods
Sprague Dawley (SD) rats were randomly divided into three groups: the control (Con) group, the type 2 diabetic (DM) group and the glucose fluctuations (GF) group. The type 2 diabetic rat model was established using a high-fat diet combined with low-dose streptozotocin injection and the GF model was induced by using staggered glucose and insulin injections daily. After eight weeks, echocardiography was used to assess the cardiac function of the three groups. Hematoxylin-eosin and Masson staining were utilized to evaluate the degree of pathological damage and fibrosis. Meanwhile, a neonatal rat cardiac fibroblast model with GF was established. Western and immunofluorescence were used to find the specific mechanism of myocardial fibrosis caused by GF.

Results
Compared with rats in the Con and the DM group, cardiac function in the GF group showed significant impairments. Additionally, the results showed that GF aggravated myocardial fibrosis in vitro and in vivo. Moreover, Ca2+/calmodulin‑dependent protein kinase II (CaMKII) was activated by phosphorylation, prompting an increase in phosphorylation of signal transducer and activator of transcription 3 (Stat3) and induced nuclear translocation. Pretreatment with KN-93 (a CaMKII inhibitor) blocked GF-induced Stat3 activation and significantly suppressed myocardial fibrosis.

Conclusions
Glucose fluctuations exacerbate myocardial fibrosis by triggering the CaMKII/Stat3 pathway in type 2 diabetes.
IK is essentially involved in ciliogenesis as an upstream regulator of oral-facial-digital syndrome ciliopathy gene, ofd1
Authors: Ka Hye In , Cho Mina , Kwon Seung-Hae , Mun Se Hwan , Han Sora , Kim Min Jung , Yang Young 
Published: October 2023

Abstract
Background
The cilia are microtubule-based organelles that protrude from the cell surface. Abnormalities in cilia result in various ciliopathies, including polycystic kidney disease (PKD), Bardet-Biedl syndrome (BBS), and oral-facial-digital syndrome type I (OFD1), which show genetic defects associated with cilia formation. Although an increasing number of human diseases is attributed to ciliary defects, the functions or regulatory mechanisms of several ciliopathy genes remain unclear. Because multi ciliated cells (MCCs) are especially deep in vivo, studying ciliogenesis is challenging. Here, we demonstrate that ik is essential for ciliogenesis in vivo.

Results
In the absence of ik, zebrafish embryos showed various ciliopathy phenotypes, such as body curvature, abnormal otoliths, and cyst formation in the kidney. RNA sequencing analysis revealed that ik positively regulated ofd1 expression required for cilium assembly. In fact, depletion of ik resulted in the downregulation of ofd1 expression with ciliary defects, and these ciliary defects in ik mutants were rescued by restoring ofd1 expression. Interestingly, ik affected ciliogenesis particularly in the proximal tubule but not in the distal tubule in the kidney.

Conclusions
This study demonstrates the role of ik in ciliogenesis in vivo for the first time. Loss of ik in zebrafish embryos displays various ciliopathy phenotypes with abnormal ciliary morphology in ciliary tissues. Our findings on the ik–ofd1 axis provide new insights into the biological function of ik in clinical ciliopathy studies in humans.
The ICU-CARB score: a novel clinical scoring system to predict carbapenem-resistant gram-negative bacteria carriage in critically ill patients upon ICU admission
Authors: Dai Yunqi , Zhang Ling , Pan Tingting , Shen Ziyun , Meng Tianjiao , Wu Jing , Gu Feifei , Wang Xiaoli , Tan Ruoming , Qu Hongping 
Published: October 2023

Abstract
Background
With the widespread spread of carbapenem-resistant gram-negative bacteria (CR-GNB) in medical facilities, the carriage of CR-GNB among critically ill patients has become a significant concern in intensive care units (ICU). This study aimed to develop a scoring system to identify CR-GNB carriers upon ICU admission.

Methods
Consecutive critically ill patients admitted to the ICU of Shanghai Ruijin Hospital between January 2017 and December 2020 were included. The patients were then divided into training and testing datasets at a 7:3 ratio. Parameters associated with CR-GNB carriage were identified using least absolute shrinkage and selection operator regression analysis. Each parameter was assigned a numerical score ranging from 0 to 100 using logistic regression analysis. Subsequently, a four-tier risk-level system was developed based on the cumulative scores, and assessed using the area under the receiver operating characteristic curve (AUC).

Results
Of the 1736 patients included in this study, the prevalence of CR-GNB carriage was 10.60%. The clinical scoring system including seven variables (neurological disease, high-risk department history, length of stay ≥ 14 days, ICU history, invasive mechanical ventilation, gastrointestinal tube placement, and carbapenem usage) exhibited promising predictive capabilities. Patients were then stratified using the scoring system, resulting in CR-GNB carriage rates of 2.4%, 12.0%, 36.1%, and 57.9% at the respective risk levels (P < 0.001). Furthermore, the AUC of the developed model in the training set was calculated to be 0.82 (95% CI, 0.78–0.86), while internal validation yielded an AUC of 0.83 (95% CI, 0.77–0.89).

Conclusions
The ICU-CARB Score serves as a straightforward and precise tool that enables prompt evaluation of the risk of CR-GNB carriage at the time of ICU admission, thereby facilitating the timely implementation of targeted pre-emptive isolation.
Five new Camillea (Xylariales) species described from French Guiana
Authors: Fournier Jacques , Hsieh Huei-Mei , Lechat Christian , Ju Yu-Ming , Chaduli Delphine , Favel Anne 
Published: October 2023

Abstract
Background
The genus Camillea was created in 1849 from collections made in French Guiana with eight species included. Numerous species assigned to Camillea were subsequently discovered, especially in the forests of the Amazon basin, but new discoveries have not been reported from French Guiana since 1849. Recent fieldwork in French Guiana has begun to fill this gap by identifying five new species, most of which were collected in the vicinity of Saül village.

Results
Based on macro- and micromorphological study of their stromata, including SEM images of ascospore wall ornamentation, five new species were recognized, including C. cribellum, C. heterostomoides, C. nitida, C. rogersii and C. saulensis. Cultures could be obtained for C. heterostomoides and C. rogersii, and ITS and LSU sequences were obtained for all of the five new species. Camillea heterostoma and its variety microspora were shown to be conspecific. Provisional molecular phylogenetic analyses support the possible reinstatement of Hypoxylon melanaspis, currently regarded as merely an applanate form of C. leprieurii.

Conclusion
The current study is based on a relatively limited fieldwork in its duration and sampling area but was able to substantially increase the number of Camillea species known from French Guiana. This augurs an exceptional and still unknown diversity of the genus in this area and by extension in the adjacent neotropical forests.
Time to death and its determinant factors of visceral leishmaniasis with HIV co-infected patients during treatment period admitted at Metema hospital, Metema, Ethiopia: a hospital-based cross-sectional study design
Authors: Alemu Chekol , Wudu Habitamu , Dessie Getu , Gashu Chalachew 
Published: October 2023

Abstract
Background
Visceral leishmaniasis is caused by the parasites Leishmania donovani spices complex that can spread to internal organs and the disease is fatal with a fatality rate of nearly 100% if left untreated. Visceral Leishmania-HIV (HIV1) coinfection disease is a new clinical form of leishmaniasis very serious disease in the endemic part of the world. It also served as the primary cause of death in the lowlands of Ethiopia with the endemic Humara and Metema that are located near the Sudanese border.

Methods
A total of 153 visceral leishmaniases with HIV co-infection secondary data was taken from the medical chart of patients from January 2015 to January 2021 and a hospital-based cross-sectional study design was carried out to retrieve relevant information. The data entered by SPSS and analysed using STATA version 14 and R4.2.1 statistical software packages using a non-parametric Model, semi-parametric Cox proportional hazard survival models at 5% significance level.

Result
Among the total visceral leishmaniasis with HIV co-infected patients 3.27% were females and 96.73% were males, 19 (12.42%) patients died and 134(87.58%) patients were censored. The Cox proportional hazard model result indicates that severe acute malnutrition, baseline CD4+ cell count ≥100, and underweight significantly contributed to the survival time of a patient. Cox proportional hazard model shows that severe acute malnutrition (HR=4.40027, 95% CI= 2.455061 262.7934, P-value=0.007), baseline CD4+cell count ≥100 (HR=0.2714623, 95% CI= 0.0764089 0.9644395, P-value=0.044), and Underweight (HR=4.678169, 95% CI= 1.970097 11.10872, P-value=0.040) significantly contributed to a shorter survival time.

Conclusion
Visceral leishmaniases with HIV co-infected patients show a large number of deaths occurred in the earlier days of treatment this implies that Visceral leishmaniasis accelerates HIV replication and disease progression death. The researcher suggests that people be aware of the burden posed by those risk factors and knowledgeable about the diseases. So, the researcher recommended that to health workers implement primary health care in those patients and careful consideration of a neglected parasitic disease.
Welfare Reform and the Quality of Young Children's Home Environments
Authors: Kalil Ariel , Corman Hope , Dave Dhaval , Schwarz-Soicher Ofira , Reichman Nancy E. 
Published: October 2023

Abstract
This study investigates the effects of welfare reform—a major policy shift in the United States that increased low-income mothers' employment and reliance on earnings instead of cash assistance—on the quality of the home environments mothers provide for their preschool-age children. Using empirical methods designed to identify plausibly causal effects, we estimate the effects of welfare reform on validated survey and observational measures of maternal behaviors that support children's cognitive skills and emotional adjustment and the material goods that parents purchase to stimulate their children's skill development. The results suggest that welfare reform did not affect the amount of time and material resources mothers devoted to cognitively stimulating activities with their young children. However, it significantly decreased emotional support provision scores, by approximately 0.3–0.4 standard deviations. The effects appear to be stronger for mothers with lower human capital. The findings provide evidence that welfare reform came at a cost to children in the form of lower quality parenting. They also underscore the importance of considering quality, and not just quantity, in assessing the effects of maternal work-incentive policies on parenting and children's home environments.
The human right to the environment in the context of the new EU Fit for 55 package
Authors: Sitek Magdalena 
Published: October 2023

Cel pracyNdMateriał i metodyndWynikindWnioskiPrzedmiotem opracowania jest analiza głównego celu pakietu UE Fit for 55 w kontekście założeń Europejskiego Zielonego Ładu. Przyjęcie przez Unię Europejską planu dość radykalnego obniżenia emisji CO2 jest zadaniem, które powinno być oceniane pozytywnie. Celem opracowania jest jednak skonfrontowanie tego zadania z prawami człowieka oraz z konfliktem zbrojnym wywołanym przez Rosję. Jako hipoteza badawcza przyjęte zostało twierdzenie, według którego wdrażanie nowych polityk czy też strategii wymaga głębokiego przemyślenia kontekstu społecznego, gospodarczego, a przede wszystkim politycznego. Analiza tak przedstawionego zagadnienia badawczego wymaga krótkiego zarysowania historii polityki ochrony środowiska oraz założeń aktualnej polityki Unii Europejskiej w tym zakresie. Efektem opracowania jest próba nakreślenia relacji jakie zachodzą pomiędzy polityką unijną ochrony środowiska a prawami człowieka i sytuacją polityczną w Ukrainie.
The impact of SARS-CoV-2 pandemic on the education process at the Police Academy in Szczytno in the opinion of respondents
Authors: Kuczyńska Ewa 
Published: October 2023

Cel pracyW Wyższej Szkole Policji w Szczytnie (WSPol) realizowane jest zadanie badawcze pt. „Szkolnictwo (nie tylko) służb mundurowych w dobie pandemii koronawirusa SARS-CoV-2 wywołującego chorobę Covid-19. Studia nad zapewnieniem ciągłości kształcenia i bezpieczeństwa podmiotom środowiska uczelni lub szkoły. Formy i rodzaje re(konstrukcji) środowiska kształcenia będącego następstwem sytuacji pandemicznej”, finansowane z subwencji MSWiA. W publikacji zaprezentowano wyniki badań odnoszące się do rozwiązań wdrożonych w pandemii w Uczelni jako dobrych praktyk do wykorzystania w sytuacji wystąpienia zagrożeń związanych m.in. z pandemią.Materiał i metodyW badaniach zastosowano metodę sondażu diagnostycznego, gdzie wykorzystano technikę anonimowej ankiety o kafeterii koniunktywnej i dysjunktywnej. Kwestionariusz ankiety powstał w formie on-line. Link rozesłano mailowo do podmiotów krajowych i zagranicznych biorących udział w badaniach.WynikiDo udziału w badaniach zaproszono 15 podmiotów, w tym 12 uczelni wyższych, wśród których znalazło się 5 uczelni krajowych i 7 zagranicznych oraz 3 szkoły zagraniczne o profilu policyjnym. Udział w badaniach wzięła też WSPol. Ostatecznie zrealizowano je w 8 podmiotach o różnym profilu działalności. Badaniu zostało poddanych 30-40% kadry dydaktycznej i 30-40% kadry kierowniczej każdej uczelni.WnioskiPandemia COVID-19 wywarła wpływ na większość dziedzin życia. Spowodowała, że również polskie uczelnie zarówno te cywilne, jak i mundurowe musiały zmierzyć się z wieloma wyzwaniami, takimi jak organizacja pracy zdalnej czy też zmiana w relacjach ze studentami/słuchaczami, bądź nauczycielami. W związku z powyższym wprowadzono bardzo szybko rozwiązania, które być może były planowane w dłuższej perspektywie czasu, jednak musiały jak najszybciej zaistnieć, gdyż taka była potrzeba.
Cognitive impairment and the associated factors among women with a history of pregnancy complications in rural southwestern Uganda
Authors: Kihumuro Raymond Bernard , Kellen Peace , Chun Sarah , Wakida Edith K. , Obua Celestino , Ainamani Herbert E. 
Published: October 2023

Background
Worldwide, there is a growing concern about the rising number of people with declining cognitive functioning. However, findings on this phenomenon are inconclusive. Our study aimed to assess the prevalence of cognitive impairment and the associated factors in women with a history of pregnancy complications in rural southwestern Uganda.

Methods
This was a cross-sectional study carried out among women above 40 years of age in the greater Kabale district of southwestern Uganda between March and April 2022. Study participants were identified using a consecutive sampling method. Predictor variables included pregnancy complications and other social demographic factors that were assessed by semi-structured interviews while cognitive functioning as an outcome variable was assessed by Montreal Cognitive Assessment (MoCA-B) tool. Data were analyzed using STATA at a 95% Confidence level. Logistic regression analyses were selected for statistical modelling while odds ratios were calculated to assess the strength of associations between the predictor and outcome variables.

Results
In total, 75% (212/280) of participants had some form of cognitive impairment, with 45% (123/280) falling into mild CI, 31% (86/280) moderate CI and 4% (10/280) severe CI. Twenty-three percent (68/280) of participants fell into category of normal cognitive functioning. Participants with >65 years of age had higher odds of developing cognitive impairment (OR = 2.94; 95%CI: 0.96–9.04, p = 0.06) than those with < 65 years of age. Protective factors to cognitive impairment include delivering from a health facility (OR = 0.31,95% CI:0.16–0.60, p = < .001), primary and post primary levels of education (OR = 0.05; 95% CI: 0.02–0.13, p<0.001, OR = 0.04; 95%CI: 0.02–0.23, p<0.001) respectively.

Conclusion
Results from this study show a high prevalence of cognitive impairment among women with a history of pregnancy complications in rural southwestern Uganda. Interventions geared toward preventing cognitive impairment among females with a history of pregnancy complications should be emphasized.