bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perinatal Nutrition and Gastrointestinal Disorders: Working Group Report of the Second World Congress of Pediatric Gastroenterology, Hepatology, and Nutrition
Authors: Kleinman Ronald E. , Berseth Carol , Castillo‐Duran Carlos , Cleghorn Geoffrey , Devane Sean , Garcia Rafael Jimenez , Ng Steven , Sanabria Marta Cristina 
Published: January 2024

Research
Promote investigation into the effects of perinatal nutrition upon long‐term health outcomes.
Identify the optimal composition of enteral formulas and parenteral nutrition solutions to meet the needs of pre‐ and full‐term infants.
Investigate the development of intestinal motility in human infants and altered motility in infancy, including better methods of diagnosis and treatment.
Intervention
Support home‐based nutritional management of chronic nutritionally debilitating illnesses during the perinatal period.
Support and encourage breastfeeding at least through the first birthday and promote dietary supplementation or fortification (according to evidence based recommendations) with trace minerals and vitamins during the pre‐ and perinatal period (zinc, folic acid, iodine, iron) in at‐risk mothers and infants.
Encourage optimal nutritional management of very low birth weight infants and infants that have undergone major surgical interventions.
Education
Enhance the integration of the nutrition curriculum into the medical school curriculum and encourage the further exchange of knowledge among perinatal nutrition experts through joint workshops and scientific meetings.
Promote the use of effective means of communications, including electronic media, to disseminate information about perinatal nutrition, including the demonstrated benefits of breastfeeding, to health care professionals and parents.
Immunoregulation in the Liver and Its Extrahepatic Relevance
Authors: O’Farrelly Cliona 
Published: January 2024

ABSTRACTAs well as being a metabolic factory, the liver also functions as a powerful immune organ constantly engaged in local immune responses. In addition, it has a significant influence on systemic immunity. The hepatic immune system recognises and responds to pathogens, as well transformed and metastatic cells, while at same time tolerising harmless dietary and commensal antigens. The molecular mechanisms by which these different discriminatory, regulatory and effector functions are mediated are only now beginning to be identified.
Prospective Population Screening for Celiac Disease: High Prevalence in the First 3 Years of Life
Authors: Castaño Luis , Blarduni Elisabeth , Ortiz Lourdes , Núñez Javier , Bilbao J. Ramón , Rica Itxaso , Martul Pedro , Vitoria Juan C. 
Published: January 2024

ABSTRACTBackground:Celiac disease (CD) is an autoimmune enteropathy that develops in genetically susceptible individuals exposed to gliadin. Early diagnosis of CD may reduce the risk of complications, and several studies have related the duration of gluten exposure to the risk of other autoimmune diseases. It has been proposed that silent CD be diagnosed as soon as possible to avoid potential complications.Objectives:The purpose of this study was to determine the prevalence of CD among children less than 3 years and to provide treatment to those patients diagnosed with CD.Patients and Methods:Parents of 1100 healthy children born between October 1998 and December 1999 were asked at the time of delivery to enroll their children in a program for the early diagnosis of CD. The parents of 830 children agreed to participate. Patients in the study were examined and anti–tissue transglutaminase antibody was first measured at about 1.5 years of age. A second antibody titer was obtained at about 2.5 years of age. Patients with detectable autoantibodies underwent intestinal biopsy for confirmation of CD.Results:Of the 830 children initially enrolled, 613 and 484 returned for the first and second visits, respectively. None had anti–tissue transglutaminase antibodies at the first visit, but 9 had anti–tissue transglutaminase immunoglobulins at the second visit. In 7 of these 9, intestinal biopsy confirmed the diagnosis of CD which suggests a minimum prevalence of CD of 1 per 118 healthy newborns.Conclusions:The authors observed a very high prevalence of CD, comparable to that observed in other European populations, which might even be higher if all of the children initially examined had returned for their second visit. If general screening for CD were accepted, the authors would recommend age 2–3 years as the best time for measuring tissue transglutaminase antibodies.
PF452 RUNX1 EXPRESSION IN NG2+ PERIVASCULAR CELLS IS REQUIRED FOR PROPER AORTIC EMBRYONIC HAEMATOPOIETIC ACTIVITY IN VIVO
Authors: Gonzalez Galofre Z.N. , Ventura T. , Crisan M. 
Published: December 2023

Background:Insight into the role of the niche in which the first haematopoietic stem cells (HSCs) emerge in vivo is still lacking. These adult‐type HSCs are generated in the ventral wall of the dorsal aorta within the aorta‐gonad‐mesonephros (AGM) region. In the bone marrow, HSCs are mainly located in close association with blood vessel walls. Cells expressing NG2, a cell surface proteoglycan found in both vasculogenic and angiogenic vasculature, have been shown to support adult HSC maintenance. Whether NG2+ cells also control HSC generation in the mouse embryo is still unknown. NG2 expression in the aortic mural cells starts shortly before HSC generation, and some NG2+ cells also express Runx1, a key haematopoietic transcription factor required for HSC generation.Aims:To investigate whether NG2+Runx1+cells control embryonic haematopoiesis in vivo.
Methods:Runx1 was deleted in NG2 expressing cells by crossing Runx1fl/fl mice with mice containing a Cre transgene under the NG2 promoter. In vitro and in vivo functional assays were performed in E10 and E11 mouse embryos to assess haematopoietic activity. Three‐dimensional (3D) confocal imaging of the intact aorta was further used to confirm our results. To investigate whether NG2+ and NG2+Runx1+ cells are haematopoietic and/or haematopoietic precursors, cell sorting and colony‐forming assays were carried out on WT, Runx1 GFP and NG2Cre‐TdTomato transgenic mice.Results:By 3D confocal imaging, we found that intra‐aortic haematopoietic clusters are dramatically reduced in number in homozygous KO mouse embryos (NG2Cre:Runx1fl/fl) compared to WT (NG2+:Runx1fl/+) controls. This was further confirmed by functional in vitro and in vivo assays. Both haematopoietic progenitors and haematopoietic stem cell activities are affected in the KO AGM. Depletion of Runx1 in NG2+ cells drastically impairs all haematopoietic progenitor numbers in the dorsal aorta of both E10.5 and E11 mouse embryos and, in vivo, KO AGM had a reduced capacity of reconstituting adult irradiated recipient mice. NG2+ cells (or NG2+Runx1+) are not haematopoietic nor haematopoietic precursors suggesting that NG2+Runx1+ cells act as a supportive niche for haematopoietic progenitor and stem cell generation at this stage.Summary/Conclusion:In conclusion, Runx1 expression in the hematopoietic niche is required prior to HPSC generation in the mouse embryo in vivo. Ongoing experiments aim to test whether this is time‐ and organ‐dependent.
PF460 SINGLE CELL RNASEQ REVEALS THE IN VIVO DIFFERENTIATION POTENTIAL AND TRANSCRIPTIONAL DYNAMICS OF IMMATURE HUMAN THYMOCYTES
Authors: Lavaert M. , Vandamme N. , Saeys Y. , Taghon T. 
Published: December 2023

Background:T cell development depends on the colonization of the thymus by multipotent progenitor cells from the bone marrow. There, these precursor cells are gradually reprogrammed into committed T cell progenitors that have lost the developmental potential towards other hematopoietic lineages. While lineage tracing experiments in mice have provided evidence to which non T‐cell lineages these early T cell precursors differentiate in vivo, it remains unclear in human which blood cell lineages can originate from early T cell precursors. In vitro differentiation experiments have revealed erythroid, myeloid (DC, monocyte, granulocyte) and lymphoid (B, T, NK) differentiation potential, but whether this occurs in vivo is not clear.Aims:While lineage tracing experiments in mice have provided evidence to which non T‐cell lineages these early T cell precursors differentiate in vivo, it remains unclear in human which blood cell lineages can originate from early T cell precursors. In vitro differentiation experiments have revealed erythroid, myeloid (DC, monocyte, granulocyte) and lymphoid (B, T, NK) differentiation potential, but whether this occurs in vivo is not clear.Methods:Therefore, we have performed single cell RNA sequencing on more than 70.000 human CD34+lin‐ thymocytes that comprise the most immature thymocyte fraction of the human pediatric thymus.Results:Analysis of this dataset showed that cell cycle constitutes the majority of variation in the dataset, indicating less heterogeneity than anticipated. Indeed, using a strategy in which both clustering and trajectory inference were used, only limited branching was observed towards other, non T‐cell hematopoietic lineages, indicating a discrepancy between reported developmental potential and in vivo cell fate. Using these branching events, commitment was traced to coincide with loss of CD44 expression, confirming recent findings. In addition, we used CITEseq to correlate previously defined immature thymocyte subsets to these newly defined developmental cell states.Summary/Conclusion:In conclusion, our work suggests that the in vivo differentiation of immature human thymocytes is much more restricted compared to what in vitro differentiation experiments have suggested. In addition, we have delineated the transcriptional events that define the transition from multipotency toward T‐cell lineage commitment at the single cell level and redefine immature thymocyte subsets in human.
PF478 DOES ESCALATED BEACOP‐DACARBAZINE DO THE SAME AS ESCALATED BEACOP‐PROCARBAZINE FOR UNFAVORABLE HODGKIN'S LYMPHOMA?
Authors: Mansouri R. , Kacem K. , Jabr D. , Bchir M. , Aissaoui L. , Berred E. , Kefi S. , Ben Néji H. , Achour M. , Ben Abdennebi Y. , Bahri M. , Meddeb B. , Ben Lakhal R. 
Published: December 2023

Background:The management of Tunisian Adult Hodgkin's lymphoma (HL) has been standardized since 1999. Since 2008 we individualized four therapeutic groups according age, stage (Ann Arbor), EORTC criteria and GHSG criteria. So for patients aged less than 60 years we considered unfavorable group who have advanced stage III‐IV Ann Arbor and II stage with large mediastinal mass. This group received the escalated BEACOPP (esc‐BEACOPP) protocol differently to other three groups.Aims:We report in this study the therapeutic results of the national protocol of Adult HL (MDH2015) for this group and demonstrate the place of Dacarbazine.Methods:Our study is prospective monocentric interesting 76 patients followed between June 2015 and December 2017 for HL stage III‐IV and stage II with large mediastinal mass and treated according MDH2015 protocol. The median age was 29 years [14–58 years].B symptoms were found in 67% of our patients. Our patients were stage III‐IV Ann Arbor in 83% of cases and stage II with large mediastinal mass in 17%. For advanced stage, 68% of patients have IPS>2. The MDH 2015 protocol is based on a strategy adapted to the therapeutic response to 2 cycles of esc‐BEACOPP. We considered as a primary failure, patients hadn’t achieved response≥75% after 4 cycles of esc‐BEACOPP.Our patients had received in this protocol Dacarbazine 250 mg/m2 three days/cycle instead of Procarbazine (esc‐BEACOPD).Results:Response≥75% to 2 courses of esc‐BEACOPD was achieved in 43% of patients and the response rate at the end of treatment was 92%. Fourteen patients (19%) had primary failure. In the multivariate analysis, the advanced stage Ann Arbor (III‐IV) and response<75% after 2 cycles of esc‐BEACOPD were an independent predictive factor of primary failure, with respectively p = 0.01 and p < 0.001. The relapse rate was 10%. The median follow‐up was 25 months [5–46 months]. Event free survival (EFS), relapse free survival (RFS) and overall survival (OS) at 2 years were respectively, 77%, 90% and 90%. In multivariate analysis, the prognostic factors for poor OS were large mediastinal mass (p = 0.05), response<75% at 2 courses (p = 0.04). The EFS is better without large mediastinal mass (p = 0.03) and without lymphopenia (p = 0.02). Fifty per cent of neutropenia was happened. Febrile neutropenia was noted in 24% of cases. No treatment related mortality was noted.Summary/Conclusion:Compared to MDH 2008 (third version of Tunisian prospective protocol), the use of Dacarbazine instead of Procarbazine is equally effective in term of response rate and the survivals (EFS, RFS and OS). The toxicity is the same.
気胸，膿胸を合併し胸腔鏡手術が有効であったLemierre症候群の1例(Pneumothorax and empyema secondary to Lemierre’s syndrome treated by thoracoscopic surgery)
Authors: 由希子 下茂 , 徹 瀧口 , 史英 金 , 太吾 阪本 , 陽介 佐藤 , 佳之 松本 , 將司 横堀 
Published: January 2024

要旨症例は既往のない29歳の男性。咽頭痛を主訴に近医を受診し，扁桃炎の診断であった。1週間後に著明なSpO2の低下を認め，集中治療目的に当科を紹介受診した。造影CTで口腔内膿瘍，左内頸静脈に血栓性静脈炎，両肺野に多数の結節影，両側の大量胸水を認めた。口腔内膿瘍の穿刺液，血液，胸水の培養からはいずれもFusobacterium necrophorumが同定された。先行する口腔咽頭感染，血栓性静脈炎，遠隔感染巣，血液培養陽性からLemierre症候群と診断した。人工呼吸器管理開始直後，両側気胸となり，酸素化の安定に時間を要した。また，その後膿胸の管理に難渋し，両側に計5本のドレーンを挿入して胸腔内の洗浄を繰り返した。左側の気胸と膿胸の改善に乏しく，第15病日に胸腔鏡補助下に洗浄ドレナージ術とドレーン位置の調整を行った。第50病日にすべてのドレーンを抜去でき，第56病日にリハビリテーション病院へ転院となった。気胸，膿胸の再発はなく社会復帰した。Lemierre症候群は，口腔，咽頭領域の先行感染を契機に発症し，炎症が波及して内頸静脈の血栓性静脈炎や遠隔臓器への敗血症性塞栓症を引き起こす重篤な全身感染症である。高頻度に敗血症性肺塞栓症を合併するため，陽圧換気による気胸発症に注意が必要である。また，合併する気胸や膿胸は難治性であることが多く，本症例では胸腔鏡を用いたドレナージが有効であった。下茂由希子と滝口徹は筆頭著者として同等の貢献をした。
Immune infiltration is associated with pan‐cancer prognostic biomarker RING finger protein 187
Authors: Fan Xin‐Tong , Gao Bing‐Fang , Wang Xiao‐Fei , Zhou Kai , Zhao Ying , Yuan Jie 
Published: January 2024

AbstractCancer is associated with the highest mortality rate globally. While life‐saving screening and treatments exist, better awareness is needed. RNF187, an E3 ligase regulating biological processes, belongs to the RING domain‐containing E3 ligase family. RNF187 may serve as an oncogene due to abnormal expression in tumors. However, its association with immune infiltration and prognosis across various cancers remains unclear. We searched several databases including TCGA, GTE x, CCLE, TIMER, and GSEA. R software was used to evaluate RNF187 differential expression, survival, pathology stage, DNA methylation, tumor mutational burden (TMB), microsatellite instability (MSI), gene co‐expression analysis, mismatch repairs (MMRs), tumor microenvironment (TME), and immune cell infiltration. Clinicopathological data were collected, and immunohistochemistry was used to verify RNF187 expression in tumor tissues. RNF187 expression was up‐regulated in various cancers compared to that in normal tissues and associated with poor patient outcomes. Dysregulation of RNF187 expression in multiple cancer types was strongly correlated with DNA methylation, MMR, MSI, and TMB. RNF187 could interact with different immune cells in cancers. Biomarkers associated with RNF187 may be helpful for prognosis and immunology in treating pan‐cancer patients.
A high‐order shell finite element for the large deformation analysis of soft material structures
Authors: Pagani A. , Augello R. , Carrera E. 
Published: January 2024

SummaryThis work proposes a higher‐order unified shell finite element for the analysis of cylinders made of compressible and nearly incompressible hyperelastic materials. The nonlinear governing equations are derived employing the Carrera unified formulation (CUF), thanks to which it is possible to build shell elements with the capability to capture three‐dimensional (3D) transverse and out‐of‐plane effects. The material and geometric nonlinearities are expressed in an orthogonal curvilinear reference system and the coupled formulation of hyperelastic constitutive law is considered. The principle of virtual work and a total Lagrangian approach is used to derive the nonlinear governing equations, which are solved by a Newton–Raphson scheme. The numerical investigations deal with a curved arch and both thick and thin cylinders subjected to line and point loadings. The obtained results are validated by comparing them with those from the literature. They demonstrate the reliability of the proposed method to analyze compressible and incompressible hyperelastic shell structures.
Rapid assessment of wetting of natural fiber surfaces by biodegradable copolyester using spectroscopic and microscopic techniques
Authors: Giri Jyoti , Henning Sven , Lach Ralf , Grellmann Wolfgang , Bledzki Andrzej K. , Adhikari Rameshwar 
Published: December 2023

AbstractA biodegradable polymer, the poly(butylene adipate co‐terephthalate) (PBAT), was compounded with the microcrystalline cellulose (MCC) obtained from different sources (such as wheat stalk and rice husk powder) to prepare the polymer composites. The two components, here found to be compatible, as evidenced by the Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Further assessment of the compounded fibers with the organic solvent, the toluene, illustrated the nature of the wetting phenomena of MCC by PBAT. The SEM observation showed the wrapping of the PBAT layer onto the MCC phase after the removal of the polymer matrix. The FTIR spectrum showed the presence of the PBAT carbonyl peak on extracted fibers from the composite via solvent removal. It could be concluded that the PBAT could wet the MCC effectively in the composites suggesting the potential of enhancing their properties.Highlights
A biodegradable polymer, the PBAT, melt‐mixed with plant‐based natural fiber.
The fiber in composites was found to be wetted with the PBAT layer.
H‐bonding between the CO group of PBAT and the OH group of fiber caused wetting.
Raw, processed, and silane‐treated fibers showed similar wetting phenomena.
The mineralocorticoid receptor forms higher order oligomers upon DNA binding
Authors: Fettweis Gregory , Johnson Thomas A. , Almeida‐Prieto Brian , Weller‐Pérez Julián , Presman Diego M. , Hager Gordon L. , Alvarez de la Rosa Diego 
Published: December 2023

AbstractThe prevailing model of steroid hormone nuclear receptor function assumes ligand‐induced homodimer formation followed by binding to DNA hormone response elements (HREs). This model has been challenged by evidence showing that the glucocorticoid receptor (GR) forms tetramers upon ligand and DNA binding, which then drive receptor‐mediated gene transactivation and transrepression. GR and the closely‐related mineralocorticoid receptors (MR) interact to transduce corticosteroid hormone signaling, but whether they share the same quaternary arrangement is unknown. Here, we used a fluorescence imaging technique, Number & Brightness, to study oligomerization in a cell system allowing real‐time analysis of receptor‐DNA interactions. Agonist‐bound MR forms tetramers in the nucleoplasm and higher order oligomers upon binding to HREs. Antagonists form intermediate‐size quaternary arrangements, suggesting that large oligomers are essential for function. Divergence between MR and GR quaternary structure is driven by different functionality of known and new multimerization interfaces, which does not preclude formation of heteromers. Thus, influencing oligomerization may be important to selectively modulate corticosteroid signaling.
A complex network of interdomain interactions underlies the conformational ensemble of monomeric TDP‐43 and modulates its phase behavior
Authors: Mohanty Priyesh , Rizuan Azamat , Kim Young C. , Fawzi Nicolas L. , Mittal Jeetain 
Published: December 2023

AbstractTAR DNA‐binding protein 43 (TDP‐43) is a multidomain protein involved in the regulation of RNA metabolism, and its aggregates have been observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Numerous studies indicate TDP‐43 can undergo liquid–liquid phase separation (LLPS) in vitro and is a component of biological condensates. Homo‐oligomerization via the folded N‐terminal domain (aa:1–77) and the conserved helical region (aa:319–341) of the disordered, C‐terminal domain is found to be an important driver of TDP‐43 phase separation. However, a comprehensive molecular view of TDP‐43 phase separation, particularly regarding the nature of heterodomain interactions, is lacking due to the challenges associated with its stability and purification. Here, we utilize all‐atom and coarse‐grained (CG) molecular dynamics (MD) simulations to uncover the network of interdomain interactions implicated in TDP‐43 phase separation. All‐atom simulations uncovered the presence of transient, interdomain interactions involving flexible linkers, RNA‐recognition motif (RRM) domains and a charged segment of disordered C‐terminal domain (CTD). CG simulations indicate these inter‐domain interactions which affect the conformational landscape of TDP‐43 in the dilute phase are also prevalent in the condensed phase. Finally, sequence and surface charge distribution analysis coupled with all‐atom simulations (at high salt) confirmed that the transient interdomain contacts are predominantly electrostatic in nature. Overall, our findings from multiscale simulations lead to a greater appreciation of the complex interaction network underlying the structural landscape and phase separation of TDP‐43.
Extrapleural air secondary to idiopathic pulmonary fibrosis‐related pneumomediastinum
Authors: Nagata Yuichi , Watanabe Takayasu , Tanabe Yuki , Kato Motoyasu , Shukuya Takehito , Seyama Kuniaki , Takahashi Kazuhisa 
Published: January 2024

Key messageExtrapleural air is a rare condition that may concurrently develop with pneumomediastinum and pneumothorax, especially in older patients with fragile connective tissues. Physicians should consider extrapleural air to prevent inadvertent harm. Coronal reconstruction computed tomography images help appreciate extrapleural air and recognize the track of extrapulmonary air.
i‐MYO: A multi‐grasp prosthetic hand control system based on gaze movements, augmented reality, and myoelectric signals
Authors: Shi Chunyuan , Zhao Jingdong , Yang Dapeng , Jiang Li 
Published: December 2023

AbstractBackgroundControlling a multi‐grasp prosthetic hand still remains a challenge. This study explores the influence of merging gaze movements and augmented reality in bionics on improving prosthetic hand control.MethodsA control system based on gaze movements, augmented reality, and myoelectric signals (i‐MYO) was proposed. In the i‐MYO, the GazeButton was introduced into the controller to detect the grasp‐type intention from the eye‐tracking signals, and the proportional velocity scheme based on the i‐MYO was used to control hand movement.ResultsThe able‐bodied subjects with no prior training successfully transferred objects in 91.6% of the cases and switched the optimal grasp types in 97.5%. The patient could successfully trigger the EMG to control the hand holding the objects in 98.7% of trials in around 3.2 s and spend around 1.3 s switching the optimal grasp types in 99.2% of trials.ConclusionsMerging gaze movements and augmented reality in bionics can widen the control bandwidth of prosthetic hand. With the help of i‐MYO, the subjects can control a prosthetic hand using six grasp types if they can manipulate two muscle signals and gaze movement.