bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal biomarkers in the differential diagnosis of ovarian tumors: the emerging roles of CA125, HE4, and C5a
Authors: Shi Huihui , Liu Liya , Deng Xueli , Xing Xiaoyu , Zhang Yan , Djouda Rebecca Yemeli , Han Liping 
Published: January 2024

Abstract
Objective
Investigating the utility of serum exosomal markers CA125, HE4, and C5a, both individually and in combination, for distinguishing between benign and malignant ovarian tumors.

Methods
In this study, we selected a total of 234 patients diagnosed with ovarian tumors, including 34 with malignant tumors, 10 with borderline ovarian tumors, and 190 with benign tumors. This study conducted comparisons of exosomal levels of CA125, HE4, and C5a among distinct groups, as well as making comparisons between serum and exosomal levels of CA125 and HE4. Furthermore, the diagnostic performance was assessed through Receiver Operating Characteristic (ROC) curve analysis. The Area Under the Curve (AUC) was computed, and a comparative evaluation of sensitivity and specificity was conducted to ascertain their effectiveness in determining the nature of ovarian tumors across different markers.

Results
Serum CA125 and HE4 levels, the ROMA index, exosomal CA125, HE4, C5a levels, and their combined applied value (OCS value) were notably elevated in the ovarian non-benign tumor group compared to the benign tumor group, with statistical significance (P < 0.05). Exosomal and serum levels of CA125 and HE4 exhibited a positive correlation, with concentrations of these markers in serum surpassing those in exosomes. The combined OCS (AUC = 0.871) for CA125, HE4, and C5a in exosomes demonstrated superior sensitivity (0.773) and specificity (0.932) compared to serum tumor markers (CA125, HE4) and the ROMA index. The tumor stage represents an autonomous risk factor influencing the prognosis of individuals with ovarian malignancies.

Conclusion
The stage of ovarian malignancy is an independent risk factor for its prognosis. The combination of exosomal CA125, HE4 and C5a has a higher clinical value for the identification of the nature of ovarian tumours.
Entecavir versus tenofovir for prevention of hepatitis B virus-associated hepatocellular carcinoma after curative resection: study protocol for a randomized, open-label trial
Authors: Pan Li-Xin , Wang Yi-Yang , Li Zhong-Hai , Luo Jia-Xi , Wu Kun-Jun , Liu Zhen-Xiu , Wu Pei-Sheng , Chen Kang , Ma Liang , Fan Xiao-Hui , Zhong Jian-Hong 
Published: January 2024

Abstract
Background
Entecavir and tenofovir disoproxil fumarate (TDF) are standard first-line treatments to prevent viral reactivation and hepatocellular carcinoma (HCC) in individuals chronically infected with the hepatitis B virus (HBV), but the long-term efficacy of the two drugs remains controversial. Also unclear is whether the drugs are effective at preventing viral reactivation or HCC recurrence after hepatectomy to treat HBV-associated HCC. This trial will compare recurrence-free survival, overall survival, viral indicators and adverse events in the long term between patients with HBV-associated HCC who receive entecavir or TDF after curative resection.

Methods
This study is a randomized, open-label trial. A total of 240 participants will be randomized 1:1 into groups receiving TDF or entecavir monotherapy. The two groups will be compared in terms of recurrence-free and overall survival at 1, 3, and 5 years after surgery; adverse events; virological response; rate of alanine transaminase normalization; and seroreactivity at 24 and 48 weeks after surgery.

Discussion
This study will compare long-term survival between patients with HBV-associated HCC who receive TDF or entecavir monotherapy. Numerous outcomes related to prognosis will be analyzed and compared in this study.

Trial registration
ClinicalTrials.gov NCT02650271. Registered on January 7, 2016.
Autophagy induces hair follicle stem cell activation and hair follicle regeneration by regulating glycolysis
Authors: Sun Pingping , Wang Zhan , Li Sixiao , Yin Jiajing , Gan Yuyang , Liu Shizhao , Lin Zhen , Wang Hailin , Fan Zhexiang , Qu Qian , Hu Zhiqi , Li Kaitao , Miao Yong 
Published: January 2024

Abstract
Background
Hair follicle stem cells (HFSCs) typically remain quiescent and are activated only during the transition from telogen to anagen to ensure that the hair follicle enters a new cycle. The metabolic behavior of stem cells in tissues is regulated by macroautophagy/autophagy, and changes in HFSC metabolism directly affect their activation and maintenance. However, the role of autophagy in the regulation of HFSC metabolism and function remains unclear.

Methods
Back skin samples were obtained from mice at different hair follicle cycle stages, and immunofluorescence staining was used to monitor autophagy in HFSCs. Mouse and human hair follicles were treated with rapamycin (Rapa, an autophagy activator) or 3-methyladenine (3-MA, an autophagy inhibitor). The effects of autophagy on the hair follicle cycle and HFSC were investigated by imaging, cell proliferation staining, and HFSC-specific marker staining. The influence and mechanism of autophagy on HFSC metabolism were explored using RNA sequencing, real-time polymerase chain reaction, immunohistochemical staining, and detection of lactate and glucose concentrations. Finally, the influence of autophagy-induced glycolysis on HFSC and the hair follicle cycle was verified by stem cell characteristics and in vivo functional experiments.

Results
Autophagy in HFSC was highest during the transition from telogen to anagen. Inhibiting autophagy with 3-MA led to early entry into catagen and prolonged telogen, whereas Rapa promoted autophagy and hair growth. Autophagy activated HFSC by increasing the expression and activity of HFSC lactate dehydrogenase (Ldha), thereby transforming HFSC metabolism into glycolysis. Inhibition of Ldha expression counteracted the effects of autophagy.

Conclusions
Autophagy activated HFSC by promoting the transition from HFSC metabolism to glycolysis, ultimately initiating the hair follicle cycle and promoting hair growth.

Graphical Abstract
Measurement properties of the EQ-5D in children and adolescents: a systematic review protocol
Authors: do Espirito Santo Caique de Melo , Santos Verônica Souza , Miyamoto Gisela Cristiane , Chiarotto Alessandro , Santos Marisa , Yamato Tiê Parma 
Published: January 2024

Abstract
Background
Although the EQ-5D instruments have been initially designed for adult populations, there are new studies evaluating and applying these instruments to children and adolescents. The EuroQol Group adapted and created two versions designed for these groups, i.e., the EQ-5D-Y versions. The measurement properties of the EQ-5D have been systematically reviewed in different health conditions. However, there is a lack of a proper systematic assessment including the studies’ risk of bias and focusing on recent studies assessing the EQ-5D instruments in children and adolescents. The lack of a systematic assessment of the EQ-5D versions does not allow us to have a comprehensive evaluation of the validity, reliability, and responsiveness of these instruments among children and adolescents. This systematic review aims to critically appraise and summarize the evidence on the measurement properties of the EQ-5D instruments (self-reported version – answered by children and adolescents; and proxy versions – versions reported by parents, caregivers, or health professionals) in children and adolescents.

Methods
A systematic review searching the following electronic databases: MEDLINE, EMBASE, CINAHL, EconLit, National Health Service Economic Evaluation Database (NHS-EED), Health Technology Assessment (HTA) database. Two independent reviewers will screen titles and abstracts and select full texts for eligibility. The COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) methodology will be followed to conduct three main assessment steps: risk of bias, quality criteria for measurement properties, and evidence synthesis.

Discussion
This systematic review will provide comprehensive information about the evidence regarding the measurement properties of EQ-5D instruments in children and adolescents of different settings and countries.

Systematic review registration
Open Science Framework with Registration https://osf.io/r8kt9/ and PROSPERO: CRD42020218382.
Using intersectional gender analysis to identify challenges in tuberculosis care at four health care facilities in Uganda
Authors: Muttamba Winters , Omongot Samson , Najjingo Irene , Nuwarinda Roseline , Buregyeya Esther , del Barrio Mariam Otmani , Morgan Rosemary , Kirenga Bruce , Ssali Sarah 
Published: January 2024

Abstract
Background
Tuberculosis (TB) care could be considered as a continuum from symptom recognition, decision to seek care, diagnosis, treatment initiation and treatment completion, with care along the continuum influenced by several factors. Gender dimensions could influence TB care, and indeed, more men than women are diagnosed with TB each year. The study was done to identify social stratifiers that intersect with gender to influence TB care.

Methods
A cross-sectional qualitative study was done at four health facilities in 3 districts in central Uganda between October 2020 and December 2020. Data was collected from patients seeking a diagnosis or on TB treatment through focus group discussions and key informant interviews. Key themes around gender guided by a gender and intersectionality lens were developed and thereafter thematic content analysis was done.

Results
Women have increased vulnerability to TB due to bio mass exposure through roles like cooking. Women have increased access to health care services as they interface with the health care system frequently given their role as child bearers and child care givers. Men have a duty to provide for their families and this most often is prioritised over healthcare seeking, and together with belief that they are powerful beings leads to poor healthcare seeking habits and delays in healthcare seeking. Decisions on when and where to seek care were not straightforward for women, who most often rely on their husbands/partners to make decisions.

Conclusions
Men and women experience challenges to TB care, and that these challenges are deeply rooted in roles assigned to them and further compounded by masculinity. These challenges need to be addressed through intersectional gender responsive interventions if TB control is to be improved.

Graphical Abstract
Identifying plant fibres in cultural heritage with optical and electron microscopy: how to present results and avoid pitfalls
Authors: Lukesova Hana , Holst Bodil 
Published: January 2024

Abstract
Identification of archaeological and historical textile fibres is important because it gives insight into resource management in former times. The arrival of new tools such as table-top scanning electron microscopes, have led to an increased interest in the topic. Unfortunately, there have been cases where a lack of documentation regarding instrument settings and selection criteria has led to questionable conclusions being drawn. Optical and scanning electron microscopy are powerful techniques, but they must be used correctly and with proper knowledge of their limitations. Furthermore, ancient fibre material is often difficult to examine due to issues such as sample degradation, mineralization and the scarcity of material, which means that conclusions based on a statistical analysis of a large number of fibres are essentially not possible. In a cultural heritage context, it is therefore essential to distinguish between characteristic features, by which we mean features that are often, but not always present in a particular species and distinguishing features, which are always present in a particular species and can therefore be used for identification even if only a small amount of sample material can be examined. We argue that the community will have to accept that, quite often, a secure identification is not possible and that absolute statements such as: “This textile is made of flax” will often have to be replaced by relative statements such as “The material is likely to be flax”. In this paper, we address these issues as follows: first, we present a fibre identification diagram which can be used, with some limitations, to distinguish between flax, hemp, nettle, jute, hops, and cotton using optical and electron microscopy. We then move on to highlight some of the typical pitfalls of using optical and electron microscopy for fibre identification. Finally, we present measurement documentation tables for optical and electron microscopy images, which we suggest should always be included in publications. Material scarcity means that the amount of material used for investigations should be kept at an absolute minimum. It is thus crucial that results are published with proper documentation so that measurements do not need to be repeated (more material is used) in future studies. It is our hope that the measurement documentation tables will be adapted by the community and used in future publications in the field. The paper finishes with a demonstration example, presenting a fibre analysis of Viking Age textile fragments from the 10th Century with documentation tables.
Research needs related to firearm rights restoration
Authors: Kafka Julie M. , Rivara Frederick P. , Ross Rachel , Rowhani-Rahbar Ali 
Published: January 2024

Abstract
Background
In the USA, firearms are commonly involved in many incidents of serious interpersonal harm. Federal law prohibits the purchase and possession of firearms by certain high-risk groups including those with prior felony or domestic violence misdemeanor convictions. Evidence supports the effectiveness of these prohibitions, but little is known about how often prohibited persons later seek to have their firearm rights restored.

Main body
For this commentary, we systematically searched the empirical literature for information about who requests firearm rights restoration in the USA, how often it is granted, and what its consequences are. We found a dearth of empirical literature on this topic.

Conclusion
We call for attention to this gap in the research. There is a need to build an evidence base that can help inform state policy and courtroom practices regarding the eligibility, appropriateness, and risk for subsequent harm following firearm rights restoration among persons who are prohibited based on a criminal conviction history.
Mastering the brain in critical conditions: an update
Authors: Robba Chiara , Zanier Elisa R. , Lopez Soto Carmen , Park Soojin , Sonneville Romain , Helbolk Raimund , Sarwal Aarti , Newcombe Virginia F. J. , van der Jagt Mathieu , Gunst Jan , Gauss Tobias , Figueiredo Samy , Duranteau Jacques , Skrifvars Markus B. , Iaquaniello Carolina , Muehlschlegel Susanne , Metaxa Victoria , Sandroni Claudio , Citerio Giuseppe , Meyfroidt Geert 
Published: January 2024

AbstractAcute brain injuries, such as traumatic brain injury and ischemic and hemorragic stroke, are a leading cause of death and disability worldwide. While characterized by clearly distict primary events—vascular damage in strokes and biomechanical damage in traumatic brain injuries—they share common secondary injury mechanisms influencing long-term outcomes. Growing evidence suggests that a more personalized approach to optimize energy substrate delivery to the injured brain and prognosticate towards families could be beneficial. In this context, continuous invasive and/or non-invasive neuromonitoring, together with clinical evaluation and neuroimaging to support strategies that optimize cerebral blood flow and metabolic delivery, as well as approaches to neuroprognostication are gaining interest. Recently, the European Society of Intensive Care Medicine organized a 2-day course focused on a practical case-based clinical approach of acute brain-injured patients in different scenarios and on future perspectives to advance the management of this population. The aim of this manuscript is to update clinicians dealing with acute brain injured patients in the intensive care unit, describing current knowledge and clinical practice based on the insights presented during this course.
Unconditional cash transfers for preterm neonates: evidence, policy implications, and next steps for research
Authors: Bouchelle Zoe , Nelin Timothy D. , Salazar Elizabeth , Duncan Andrea F. , Parker Margaret G. 
Published: January 2024

AbstractTo address socioeconomic disparities in the health outcomes of preterm infants, we must move beyond describing these disparities and focus on the development and implementation of interventions that disrupt the factors contributing to them. Unconditional cash transfers (UCTs), which provide unrestricted payments to individuals or households, can help mitigate income disparities and improve health outcomes. While UCTs have been utilized for other vulnerable populations, their full potential has yet to be realized for low-income families with preterm infants, who face significant financial strain. In this perspective, we review evidence supporting UCTs as an intervention for children in the U.S. (including those born term and preterm), discuss the potential benefits of recurring UCTs to low-income families of preterm infants, and propose a conceptual model through which UCTs may improve outcomes for preterm infants. We conclude with potential policy levers for implementing UCTs and key unanswered questions for researchers.
First assessment of weeks-to-negative processing fluids in breeding herds after a Senecavirus A outbreak
Authors: Preis Guilherme , Benjamin Neal R , Murray Deborah , Taylor Emily Byers , Copeland Samuel , Allison Grant , Corzo Cesar A 
Published: January 2024

AbstractSenecavirus A (SVA) causes vesicular disease in swine and has been responsible for a rampant increase in the yearly number of foreign animal disease investigations conducted in the United States. Diagnostic investigations for SVA are typically performed by sampling animals individually, which is labor-intensive and stressful. Developing an alternative aggregate sampling method would facilitate the detection of this virus at the population level. In a preliminary study, SVA was detected in processing fluids (PF) collected in a breeding herd before and after outbreak detection. The objective of this study was to estimate the average number of weeks PF remain SVA-positive after an SVA outbreak. Ten farrow-to-wean breeding herds volunteered to participate in this studyby longitudinally collecting PF samples after an SVA outbreak was detected and submitting samples for RT-rtPCR testing. The PF samples from the 10 farms were SVA-positive for an average of 11.8 weeks after the outbreak. Here, we show that testing of PF may be a cost-effective method to detect SVA and help halt its spread in SVA-endemic regions.
Life experiences and cultural adaptation among migrant workers in Malaysia
Authors: Enh Azlizan Mat , Wahab Andika , Azlan Arina Anis , Talib Kartini Aboo , Sakti Andi Muhammad Tri , Sultan Fazal Mohamed Mohamed 
Published: January 2024

AbstractThis study examines the state of migrants’ cultural adaptation in Malaysia, and how such an adaptation can help build our understanding of migrants’ life and employment experiences in the country. In doing so, this study has adopted a quantitative approach, with a completed survey towards 410 migrant respondents, living and working temporarily in Selangor, Malaysia. A multiple regression analysis finds that the three most significant predictors contributing to the respondents’ cultural adaptation are “positive experiences” (β = .677, p = .000), “closeness” (β = − .107, p = .008), and “social relationships” (β = .095, p = .032). While “positive experiences” and “social relationships” influence the migrant workers’ adaptation positively, the “closeness” predictor on the contrary (negative). Another predictor, “disconnection”, is found to be not statistically significant. The one-way analysis of variance (ANOVA) reveals significant differences in the respondents’ cultural adaptation based on such demographic characteristics as age, gender, level of education, nationality, length of employment, and sector of employment. For instance, female migrants are strongly associated with a higher level of “positive experiences” [F(1, 408) = 6.321, p = .013] and “social relationships” [F(1, 408) = 5.634, p = .018], while male migrants tend to rely on cultural proximity (i.e., “closeness”) [F(1, 408) = 6.828, p = .009]. The discussion section highlights attributes such as the gender factor in cultural adaptation, preservation of cultural identities, and creation of migrants’ symbolic places to understand how cultural adaptation intersects with the migrant workers’ daily lives and experiences. This study concludes that as Malaysia’s economy continues to rely on migrant workers, it needs to better understand the workers’ cultural adaptation and their far-reaching impact on their life experiences and employment conditions in the country.
Anti-complement drugs for the treatment of geographic atrophy and the release of silicone oil
Authors: Melo Gustavo Barreto , Emerson Geoffrey Guy 
Published: January 2024

AbstractIntravitreal injections are a common procedure in ophthalmology, often using syringes coated with silicone to aid piston movement and needles coated with silicone oil to facilitate penetration of the sclera. Pegcetacoplan and avacincaptad pegol, recently approved for clinical use by the US Food and Drug Administration, have higher viscosity and seem more susceptible to entrap air bubbles compared to anti-VEGF drugs.It is plausible that both anti-complement drugs could be associated with a higher likelihood of introducing silicone oil in the vitreous because of higher viscosity, with potentially higher friction at the inner surface of syringe barrel, in the vicinity of silicone oil. In addition to this, undesirable agitation might be inadvertently promoted by some retina specialists to remove air bubbles from the drug solution.In conclusion, recent reports of silicone oil droplets in the vitreous of patients receiving pegcetacoplan injection might be related to both its viscosity and to agitation of the syringe to remove air bubbles. Since avacincaptad pegol also is viscous, though with different pH, syringe and filter needle, we might expect similar reports for this agent soon. We also recommend further studies be carried not only to clarify the current matter but also the potential association between the combination of agitation, silicone oil and inflammation or any immune response.
What wages do people expect for vocational and academic education backgrounds in Switzerland?
Authors: Cattaneo Maria Alejandra 
Published: January 2024

AbstractCorrectly anticipating the earnings for different education profiles is pivotal in making informed education decisions. In this paper, leveraging unique survey data, we study the wage expectations for academic and vocational education backgrounds in Switzerland. Personal reference points matter in forming these wage expectations as we find significant heterogeneity in their distributions by gender, age, socioeconomic status, region of residence, and migration background. Asymmetries exist between beliefs for academic and vocational backgrounds since relative differences in wage expectations also vary by respondents’ characteristics. These heterogeneities are vital for education policy because our analyses show that the wage expectations are associated with preferences for specific educational tracks for the own (hypothetical) child. If education decisions are ill-informed, this possibly leads to educational mismatches and related adverse effects later in life.