bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective affirmative therapeutics of cannabidiol oil mitigates doxorubicin-induced abnormalities in kidney function, inflammation, and renal tissue changes
Authors: Soliman Nabil A. , Dahmy Samih I. El , Shalaby Amr A. , Mohammed Khadija A. 
Published: November 2023

AbstractNephropathy is the decline in kidney function. A promising treatment for numerous types of illness is using natural materials as natural chemical compounds. The inquiry was conducted to investigate cannabidiol (CBD) potential for renal syndrome protection. The five equal groups of fifty male Sprague-Dawley rats weighing 150 ± 25 g each were designed; group I received distilled water orally, while group II got an intraperitoneal injection of doxorubicin (18 mg/kg bwt). Group III received CBD (26 mg/kg bwt) orally, while group IV received 1 ml of CBD (26 mg/kg bwt) and group V received trimetazidine (10 mg/kg bwt), in addition to a single intraperitoneal dose of doxorubicin (18 mg/kg bwt) on the 11th day for both groups (IV, V). The administration of CBD (26 mg/kg bwt) led to a noticeable improvement in oxidative stress parameters (SOD and GSH) in rats by significantly lowering enzyme activity (ALT and AST), as well as serum creatinine and urea, IL-6, and MDA, confirming the anti-inflammatory accuracy of CBD linked to significant lowering to IL6R DNA frequency concentration in line with histopathology results. As a result of its anti-inflammatory and antioxidant capabilities, cannabidiol may have protective quality, and CBD medication could be related to controlling renal problems.
Automated micro-solid-phase extraction clean-up and gas chromatography-tandem mass spectrometry analysis of pesticides in foods extracted with ethyl acetate
Authors: Schürmann Andreas , Crüzer Claudio , Duss Veronika , Kämpf Robin , Preiswerk Thomi , Huebschmann Hans-Joachim 
Published: November 2023

AbstractGeneric extraction methods for the multi-compound pesticide analysis of food have found their solid place in laboratories. Ethyl acetate and acetonitrile extraction methods have been developed as fast and easy to handle standard multi-compound methods, both feature benefits and limitations. The direct injection to gas chromatography can be impaired by a high burden of coextracted matrix, resulting in deterioration of the chromatographic system and matrix effects, requiring frequent maintenance. Therefore, common clean-up methods, such as dispersive solid-phase extraction, freeze-out of fats, or gel permeation chromatography, have been applied in clean-up. Automated clean-up using micro-solid-phase extraction (µSPE) is a recent development with several demonstrated advantages when employed in the analysis of pesticides and other contaminants in foods extracted with acetonitrile, but it has not yet been evaluated in this application using ethyl acetate for extraction. In this study, an automated procedure using µSPE cartridges was developed and established on an x,y,z robotic sampler for the raw extract clean-up and preparation of diluted samples for injection on a GC-MS/MS system. Validation experiments for 212 pesticides, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons in lettuce, avocado, raspberry, paprika, egg, and liver extracts were performed using µSPE with MgSO4, PSA, C18, and CarbonX. The performance in routine operation is briefly discussed.
Graphical abstract
Targeting alternative splicing as a new cancer immunotherapy-phosphorylation of serine arginine-rich splicing factor (SRSF1) by SR protein kinase 1 (SRPK1) regulates alternative splicing of PD1 to generate a soluble antagonistic isoform that prevents T cell exhaustion
Authors: Wahid Mussarat , Pratoomthai Benjamart , Egbuniwe Isioma U. , Evans Hannah R. , Babaei-Jadidi Roya , Amartey Jason O. , Erdelyi Viola , Yacqub-Usman Kiren , Jackson Andrew M. , Morris Jonathan C. , Patel Poulam M. , Bates David O. 
Published: November 2023

Abstract
Background
Regulation of alternative splicing is a new therapeutic approach in cancer. The programmed cell death receptor 1 (PD-1) is an immunoinhibitory receptor expressed on immune cells that binds to its ligands, PD-L1 and PD-L2 expressed by cancer cells forming a dominant immune checkpoint pathway in the tumour microenvironment. Targeting this pathway using blocking antibodies (nivolumab and pembrolizumab) is the mainstay of anti-cancer immunotherapies, restoring the function of exhausted T cells. PD-1 is alternatively spliced to form isoforms that are either transmembrane signalling receptors (flPD1) that mediate T cell death by binding to the ligand, PD-L1 or an alternatively spliced, soluble, variant that lacks the transmembrane domain.

Methods
We used PCR and western blotting on primary peripheral blood mononuclear cells (PBMCs) and Jurkat T cells, IL-2 ELISA, flow cytometry, co-culture of melanoma and cholangiocarcinoma cells, and bioinformatics analysis and molecular cloning to examine the mechanism of splicing of PD1 and its consequence.

Results
The soluble form of PD-1, generated by skipping exon 3 (∆Ex3PD1), was endogenously expressed in PBMCs and T cells and prevents cancer cell-mediated T cell repression. Multiple binding sites of SRSF1 are adjacent to PD-1 exon 3 splicing sites. Overexpression of phosphomimic SRSF1 resulted in preferential expression of flPD1. Inhibition of SRSF1 phosphorylation both by SRPK1 shRNA knockdown and by a selective inhibitor, SPHINX31, resulted in a switch in splicing to ∆Ex3PD1. Cholangiocarcinoma cell-mediated repression of T cell IL-2 expression was reversed by SPHINX31 (equivalent to pembrolizumab).

Conclusions
These results indicate that switching of the splicing decision from flPD1 to ∆Ex3PD1 by targeting SRPK1 could represent a potential novel mechanism of immune checkpoint inhibition in cancer.
Geometric Blow-Up for Folded Limit Cycle Manifolds in Three Time-Scale Systems
Authors: Jelbart S. , Kuehn C. , Kuntz S.-V. 
Published: November 2023

AbstractGeometric singular perturbation theory provides a powerful mathematical framework for the analysis of ‘stationary’ multiple time-scale systems which possess a critical manifold, i.e. a smooth manifold of steady states for the limiting fast subsystem, particularly when combined with a method of desingularisation known as blow-up. The theory for ‘oscillatory’ multiple time-scale systems which possess a limit cycle manifold instead of (or in addition to) a critical manifold is less developed, particularly in the non-normally hyperbolic regime. We use the blow-up method to analyse the global oscillatory transition near a regular folded limit cycle manifold in a class of three time-scale ‘semi-oscillatory’ systems with two small parameters. The systems considered behave like oscillatory systems as the smallest perturbation parameter tends to zero, and stationary systems as both perturbation parameters tend to zero. The additional time-scale structure is crucial for the applicability of the blow-up method, which cannot be applied directly to the two time-scale oscillatory counterpart of the problem. Our methods allow us to describe the asymptotics and strong contractivity of all solutions which traverse a neighbourhood of the global singularity. Our main results cover a range of different cases with respect to the relative time-scale of the angular dynamics and the parameter drift. We demonstrate the applicability of our results for systems with periodic forcing in the slow equation, in particular for a class of Liénard equations. Finally, we consider a toy model used to study tipping phenomena in climate systems with periodic forcing in the fast equation, which violates the conditions of our main results, in order to demonstrate the applicability of classical (two time-scale) theory for problems of this kind.
The new European Society of Cardiology guideline for the management of cardiomyopathies: key messages for cardiac electrophysiologists
Authors: Korthals Dennis , Eckardt Lars 
Published: November 2023

AbstractElectrocardiographic findings and arrhythmias are common in cardiomyopathies. Both may be an early indication of a specific diagnosis or may occur due to myocardial fibrosis and/or reduced contractility. Brady- and tachyarrhythmias significantly contribute to increased morbidity and mortality in patients with cardiomyopathies. Antiarrhythmic therapy including risk stratification is often challenging and plays a major role for these patients. Thus, an “electrophysiological” perspective on guidelines on cardiomyopathies may be warranted. As the European Society of Cardiology (ESC) has recently published a new guideline for the management of cardiomyopathies, this overview aims to present key messages of these guidelines. Innovations include a new phenotype-based classification system with emphasis on a multimodal imaging approach for diagnosis and risk stratification. The guideline includes detailed chapters on dilated and hypertrophic cardiomyopathy and their phenocopies, arrhythmogenic right ventricular cardiomyopathy, and restrictive cardiomyopathy as well as syndromic and metabolic cardiomyopathies. Patient pathways guide clinicians from the initial presentation to diagnosis. The role of cardiovascular magnetic resonance imaging and genetic testing during diagnostic work-up is stressed. Concepts of rhythm and rate control for atrial fibrillation have led to new recommendations, and the role of defibrillator therapy in primary prevention is discussed in detail. Whilst providing general guidelines for management, the primary objective of the guideline is to ascertain the disease etiology and disease-specific, individualized management.
Grounded L-Graphs Are Polynomially $$\chi $$-Bounded
Authors: Davies James , Krawczyk Tomasz , McCarty Rose , Walczak Bartosz 
Published: November 2023

AbstractA grounded L-graph is the intersection graph of a collection of “L” shapes whose topmost points belong to a common horizontal line. We prove that every grounded L-graph with clique number $$\omega $$
ω
has chromatic number at most $$17\omega ^4$$

17

ω
4

. This improves the doubly-exponential bound of McGuinness and generalizes the recent result that the class of circle graphs is polynomially $$\chi $$
χ
-bounded. We also survey $$\chi $$
χ
-boundedness problems for grounded geometric intersection graphs and give a high-level overview of recent techniques to obtain polynomial bounds.
Incidence, severity, and temporal development of oral complications in pediatric allogeneic hematopoietic stem cell transplant patients – a multicenter study
Authors: Agholme Monica Barr , Dahllöf Göran , Törlén Johan Karlsson , Majorana Alessandra , Brennan Michael T. , von Bültzingslöwen Inger , Tan Poh Lin , Hu Shijia , Sim Yu Fan , Hong Catherine 
Published: November 2023

Abstract
Purpose
Oral mucositis is a common complication for patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) and causes pain and difficulties in functions like eating and swallowing, resulting in lower quality of life and greater need of treatment with opioids and parenteral nutrition. This prospective multicenter study focused on pediatric recipients of HSCT in the neutropenic phase concerning oral complications, timing, severity, and patient experience.

Methods
The cohort comprised 68 patients, median age 11.1 years (IQR 6.3) receiving allogeneic HSCT at three clinical sites. Medical records were retrieved for therapy regimens, concomitant medications, oral and dental history, and subjective oral complaints. Calibrated dentists conducted an oral and dental investigation before HSCT. After HSCT graft infusion, study personnel made bedside assessments and patients filled out a questionnaire once or twice a week until neutrophil engraftment.

Results
We followed 63 patients through the neutropenic phase until engraftment. 50% developed oral mucositis of grades 2–4. Peak severity occurred at 8–11 days after stem cell infusion. Altogether, 87% had subjective oral complaints. The temporal distribution of adverse events is similar to the development of oral mucositis. The most bothersome symptoms were blisters and oral ulcerations, including mucositis; 40% reported severe pain and major impact on activities of daily living despite continuous use of opioids.

Conclusion
This study highlights the burden of oral complications and their negative effect on the health and quality of life of HSCT recipients.
Eigenvalues of truncated unitary matrices: disk counting statistics
Authors: Ameur Yacin , Charlier Christophe , Moreillon Philippe 
Published: November 2023

AbstractLet T be an $$n\times n$$

n
×
n

truncation of an $$(n+\alpha )\times (n+\alpha )$$

(
n
+
α
)
×
(
n
+
α
)

Haar distributed unitary matrix. We consider the disk counting statistics of the eigenvalues of T. We prove that as $$n\rightarrow + \infty $$

n
→
+
∞

with $$\alpha $$
α
fixed, the associated moment generating function enjoys asymptotics of the form $$\begin{aligned} \exp \big ( C_{1} n + C_{2} + o(1) \big ), \end{aligned}$$

exp

(

C
1

n
+

C
2

+
o

(
1
)

)

,

where the constants $$C_{1}$$

C
1

and $$C_{2}$$

C
2

are given in terms of the incomplete Gamma function. Our proof uses the uniform asymptotics of the incomplete Beta function.
The impact of intraoperative MRI on cranial surgical site infections—a single-center analysis
Authors: Joerger Ann-Kathrin , Laho Xhimi , Kehl Victoria , Meyer Bernhard , Krieg Sandro M. , Ille Sebastian 
Published: November 2023

Abstract
Purpose
The use of intraoperative MRI (ioMRI) contributes to an improved extent of resection. Hybrid operating room MRI suites have been established, with the patient being transferred to the MRI scanner. In the present descriptive analysis, we compared the rate of surgical site infections (SSI) after intracranial tumor surgery with and without the use of ioMRI.

Methods
In this retrospective study, we included 446 patients with open craniotomy performed for brain tumor surgery. One hundred fourteen patients were operated on with the use of ioMRI between June 1, 2018, and June 30, 2019 (group 1). During the same period, 126 patients were operated on without ioMRI (group 2). As an additional control group, we analyzed 206 patients operated on from February 1, 2017, to February 28, 2018 when ioMRI had not yet been implemented (group 3).

Results
The rate of SSI in group 1 (11.4%), group 2 (9.5%), and group 3 (6.8%) did not differ significantly (p = 0.352). Additional resection after ioMRI did not result in a significantly elevated number of SSI. No significant influence of re-resection, prior radio-/chemotherapy, blood loss or duration of surgery was found on the incidence of SSI.

Conclusion
Despite the transfer to a non-sterile MRI scanner, leading to a prolonged operation time, SSI rates with and without the use of ioMRI did not differ significantly. Hence, advantages of ioMRI outweigh potential disadvantages as confirmed by this real-life single-center study.
Genomic insights into the adaptation of Acinetobacter johnsonii RB2-047 to the heavy metal-contaminated subsurface mine environment
Authors: Timková Ivana , Maliničová Lenka , Nosáľová Lea , Kolesárová Mariana , Lorková Zuzana , Petrová Nikola , Pristaš Peter , Kisková Jana 
Published: November 2023

AbstractThe subsurface mine environments characterized by high levels of toxic metals and low nutrient availability represent an extreme threat to bacterial persistence. In recent study, the genomic analysis of the Acinetobacter johnsonii strain RB2-047 isolated from the Rozália Gold Mine in Slovakia was performed. As expected, the studied isolate showed a high level of heavy metal tolerance (minimum inhibitory concentrations were 500 mg/L for copper and nickel, 1,500 mg/L for lead, and 250 mg/L for zinc). The RB2-047 strain also showed noticeable resistance to several antibiotics (ampicillin, kanamycin, chloramphenicol, tetracycline and ciprofloxacin). The genomic composition analysis demonstrated a low number of antibiotic and metal resistance coding genes, but a high occurrence of efflux transporter genes located on the bacterial chromosome. The experimental inhibition of efflux pumps resulted in decreased tolerance to Zn and Ni (but not to Cu and Pb) and to all antibiotics tested. In addition, the H33342 dye-accumulation assay confirmed the high efflux activity in the RB2-047 isolate. These findings showed the important role of efflux pumps in the adaptation of Acinetobacter johsonii strain RB2-047 to metal polluted mine environment as well as in development of multi-antibiotic resistance.
Automatic genre identification: a survey
Authors: Kuzman Taja , Ljubešić Nikola 
Published: November 2023

AbstractAutomatic genre identification (AGI) is a text classification task focused on genres, i.e., text categories defined by the author’s purpose, common function of the text, and the text’s conventional form. Obtaining genre information has been shown to be beneficial for a wide range of disciplines, including linguistics, corpus linguistics, computational linguistics, natural language processing, information retrieval and information security. Consequently, in the past 20 years, numerous researchers have collected genre datasets with the aim to develop an efficient genre classifier. However, their approaches to the definition of genre schemata, data collection and manual annotation vary substantially, resulting in significantly different datasets. As most AGI experiments are dataset-dependent, a sufficient understanding of the differences between the available genre datasets is of great importance for the researchers venturing into this area. In this paper, we present a detailed overview of different approaches to each of the steps of the AGI task, from the definition of the genre concept and the genre schema, to the dataset collection and annotation methods, and, finally, to machine learning strategies. Special focus is dedicated to the description of the most relevant genre schemata and datasets, and details on the availability of all of the datasets are provided. In addition, the paper presents the recent advances in machine learning approaches to automatic genre identification, and concludes with proposing the directions towards developing a stable multilingual genre classifier.
Global warming vs. climate change frames: revisiting framing effects based on new experimental evidence collected in 30 European countries
Authors: Stefkovics Ádám , Zenovitz Lili 
Published: November 2023

AbstractExisting evidence suggests that climate change beliefs can be subject to how the issue is framed. Particularly, the choice between a “global warming” versus a “climate change” framing influenced survey responses in some previous experiments. Furthermore, since the issue of climate change has become strongly polarized politically, framing effects were found to be moderated by political identification. Nevertheless, most of these framing effects were observed in the USA and may not be generalized in other cultures. To contribute to this area of research, we embedded an experiment in nationally representative telephone surveys conducted in 30 European countries in August–September 2021. We manipulated the wording of four climate change-related questions (“global warming” vs. “climate change”) and assessed treatment effects both in the full sample and separately among leftist, rightist, green, and right-wing party voters. Our results regarding climate change existence belief replicated earlier findings since existence belief was significantly higher in the climate change group. We also found that the perceived negative impact was slightly lower when the issue was framed as “climate change.” In contrast, little evidence was found of people being more skeptical or less worried when asked about “global warming” compared to “climate change.” In general, skepticism was higher on the political right; however, leftists, rightists, green, and right-wing voters reacted similarly to the two frames in our European sample. We consider possible explanations for the null findings and argue that the use of “climate change” framing in communication strategies promoting climate action itself will do little to lower skepticism in Europe.
Analysis of micro- and nanoplastics in wastewater treatment plants: key steps and environmental risk considerations
Authors: Cavazzoli Simone , Ferrentino Roberta , Scopetani Costanza , Monperrus Mathilde , Andreottola Gianni 
Published: November 2023

AbstractThe analysis of micro- and nanoplastics (MNPs) in the environment is a critical objective due to their ubiquitous presence in natural habitats, as well as their occurrence in various food, beverage, and organism matrices. MNPs pose significant concerns due to their direct toxicological effects and their potential to serve as carriers for hazardous organic/inorganic contaminants and pathogens, thereby posing risks to both human health and ecosystem integrity. Understanding the fate of MNPs within wastewater treatment plants (WWTPs) holds paramount importance, as these facilities can be significant sources of MNP emissions. Additionally, during wastewater purification processes, MNPs can accumulate contaminants and pathogens, potentially transferring them into receiving water bodies. Hence, establishing a robust analytical framework encompassing sampling, extraction, and instrumental analysis is indispensable for monitoring MNP pollution and assessing associated risks. This comprehensive review critically evaluates the strengths and limitations of commonly employed methods for studying MNPs in wastewater, sludge, and analogous environmental samples. Furthermore, this paper proposes potential solutions to address identified methodological shortcomings. Lastly, a dedicated section investigates the association of plastic particles with chemicals and pathogens, alongside the analytical techniques employed to study such interactions. The insights generated from this work can be valuable reference material for both the scientific research community and environmental monitoring and management authorities.
Graphical Abstract