bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Challenging Cases series: Novel Options for Refractory Cancer Therapy-induced Thrombocytopenia（r-CTIT）
Authors: Hong Li-Li , Wen Qiang , Zhou Hai-Li , Wang Shou-Jun , Chen Kai-Li , Mu Jie , Shen Siyu , Zhuang Xiao-Fen , Wang Chun-Feng , Leng Ya-Chun , Wang Yong-Jun , Zhuang Hai-Feng 
Published: January 2024

Abstract

Purpose
Refractory cancer therapy-induced thrombocytopenia (r-CTIT) presents a life-threatening complication of tumor therapy, for which there is currently no established and effective long-term treatment.
Methods
Umbilical cord blood (UCB) is rich in hematopoietic stem cells with multi-lineage differentiation potential, while umbilical cord mesenchymal stem cells (UC-MSCs) have been shown to support megakaryocytopoiesis and regulate the bone marrow microenvironment. The components of cells have demonstrated great value in the treatment of hematopoietic failure diseases, especially in patients with r-CTIT.To investigate the impact of intravenous sequential infusion of UCB and UC-MSCs in patients with r-CTIT, we conducted a follow-up study.
Results
Among the 13 patients evaluated, the platelet response rate was 84.6% (11/13). Complete response (CR) was observed in 53.8% (7/13) of the patients, while partial response (PR) was seen in 84.6% (9/13) of the patients. The median time for platelet recovery in all patients was 53 days (range: 6-217 days). Our findings revealed that the infusion of UCB and UC-MSCs in r-CTIT patients has shown positive effects on erythroid and neutrophil levels, with an 84.6% erythroid response rate (11/13) and a 92.3% improvement in neutrophil count. Furthermore, no severe adverse reactions to the infusion were observed.
Conclusions
Based on our study, we can conclude that the sequential infusion of UCB and UC-MSCs can be a novel and promising therapeutic approach for r-CTIT patients.
Genetic and Molecular Correlates of Cortical Thickness Alterations in Adults with Obsessive-Compulsive Disorder: A Transcription-Neuroimaging Association Analysis
Authors: Hu Xiao , Zhang Da , Teng Chang , Xu Yin , Tian Lei , Cao Ping , Wang Xiao , Li Zong , Guan Cheng 
Published: January 2024

Abstract
Although numerous neuroimaging studies have shown neural alterations in individuals with obsessive-compulsive disorder (OCD), a psychiatric disorder characterised by intrusive cognitions and repetitive behaviours, the molecular mechanisms linking brain structural changes and gene expression remain poorly understood. By combining the Allen Human Brain Atlas dataset with neuroimaging data from the Meta-Analysis (ENIGMA) consortium and independent cohorts, this study performed partial least squares regression and enrichment analysis to probe the correlation between transcription and cortical thickness variation in adults with OCD. The cortical map of case–control differences in cortical thickness was spatially correlated with cortical expression of a weighted combination of genes enriched for neurobiologically relevant ontology terms, preferentially expressed across different cell types and cortical layers. These genes were specifically expressed in brain tissue, spanning nearly all cortical developmental stages. Protein-protein interaction analysis revealed that these genes coded a network of proteins encompassing several highly interactive hubs. The findings of this study bridge the gap between neural structure and transcriptome data in OCD, fostering an integrative understanding of the potential biological mechanisms.
The Impact of Urban Health Services on the Residency Intentions ofOlder Migrant Workers
Authors: Pang Zhi , Xin Yi , Wang Jinyang 
Published: January 2024

Abstract
Objective
Migrant workers play a crucial role in China's urbanization, yet many are reluctant to stay in the cities where they have worked hard in their old age. This study analyzes the impact of health service accessibility on the residency intentions of older migrant workers. Using the concept of equalization, it proposes new requirements for the health security system, which is significant for the future construction of new urbanization in China and the strategic deployment of the aging migrant population.
Methods
This study mainly uses the 2017 Migrant Population Dynamic Monitoring Survey data released by the National Health and Family Planning Commission. It describes and analyzes health services in terms of spatial accessibility, appropriateness, and availability using descriptive statistics and binary Logistic regression analysis.
Results
Establishing health records and receiving health education are the main factors of health services that influence the residency intentions of migrant workers.
Conclusion
Improving the level of medical and health services can further enhance the residency intentions of older migrant workers. Under the strategic layout of Healthy China and the equalization of medical and health services, it is necessary to continue examining and improving the health service conditions of migrant workers to overcome the current dilemma of "flowing without staying".
Clinical indicators and reproductive tract microbiota abnormalities indicate the occurrence of endometriosis
Authors: Li Xiaoqing , Chen Cong , Zheng Yuanyuan , Lin Wenjing , Zhang Hongping , Pan Qionghui 
Published: January 2024

Abstract

Endometriosis is an inflammation-associated disease, primarily but not always associated with abnormal immune system function and expression of immune factors. The microbiota of the female reproductive tract, including the vagina and cervix, plays a crucial role in health and disease. The immune dysregulation caused by the imbalance of reproductive tract microbiota may contribute to endometriosis. In this study, 35 women was recruited, including 17 women with endometriosis and 18 healthy women, while their general clinical data, cervical secretions and vaginal secretions were collected. High-throughput sequencing technology was performed to analyze the cervical and vaginal microbiota. We found that patients with endometriosis have unique vaginal and cervical microbiota. Romboutsia, Ruminococcus, Phascolarctobacterium, and Olsenella in the reproductive tract had significant positive correlation with the visual analogue scale index for endometriosis, while Mobiluncus displayed a significant negative correlation with the visual analogue scale index, and Lactobacillus showed a significant negative correlation with the thyroid peroxidase antibody index. These clinical and microbiological indicators might be associated with endometriosis, and this study has clinical significance for the detection and prevention of endometriosis.
Values and preferences towards the use of prophylactic low-molecular-weight heparin during pregnancy: A convergent mixed-methods secondary analysis of data from the Decision Analysis in SHared decision making for Thromboprophylaxis during Pregnancy (DASH-TOP) study
Authors: León‑García Montserrat , Humphries Brittany , Xie Feng , Gravholt Derek , Golembiewski Elizabeth , Eckman Mark H. , Bates Shannon M. , Hargraves Ian , Pelayo Irene , López Sandra Redondo , Caño Juan Antonio Millón , Alcántara Milagros A. Suito , D'Souza Rohan , Shehata Nadine , Jack Susan M , Guyatt Gordon , Perestelo‑Perez Lilisbeth , Alonso‑Coello Pablo 
Published: January 2024

Abstract

Background:
Venous thromboembolism (VTE) in pregnancy is a major cause of maternal morbidity and mortality, and the use of preventive low-molecular-weight heparin (LMWH) can be challenging. Clinical guidelines recommend eliciting pregnant individuals’ preferences towards the use of daily injections of LMWH and discussing the best option through a shared decision-making (SDM) approach. Our aim was to identify individuals’ preferences concerning each of the main clinical outcomes, and categorize attributes influencing the use of LMWH during pregnancy.
Methods:
Design: Convergent mixed-methods.
Participants: Pregnant women or those planning a pregnancy with VTE recurrence risk.
Intervention: A SDM intervention about thromboprophylaxis with LMWH in pregnancy.
Analysis: Quantitatively, we report preference scores assigned to each of the health states. Qualitatively, we categorized preference attributes using Burke’s pentad of motives framework: scene, agent, agency, act, and purpose. We use mixed-method convergent analysis to report findings using side-by-side comparison of concordance/discordance.
Results:
The least valued health state was to experience a pulmonary embolism (PE), followed by major obstetrical bleeding (MOB), deep vein thrombosis (DVT), and using daily injections of LMWH (valued as closest to a ‘healthy pregnancy’). Women's previous experiences, access to care (scene) and shared decision-making (agent) affected preferences. LMWH's benefits were noted, but substantial drawbacks described (agency). The main goal was avoiding the risk of VTE (purpose). Side-by-side comparisons revealed concordance between motives and DVT and PE health states. Discordance appeared between using daily injections of LMWH and agent- and agency motives and between MOB and the agency motive.
Conclusions:
Mixed-methods provide a nuanced understanding of women's LMWH preferences, by quantifying health states preferences and exploring attributes qualitatively. Incorporating both methods may improve patient-centered care around preference-sensitive decisions in thromboprophylaxis during pregnancy.
Not all MYC FISH probes are created equal
Authors: Dupuy Amanda K , Tripodi Joseph , Mizhiritskaya Valentina , Bezugly Mariya , Karolczuk Ewa , Najfeld Vesna 
Published: January 2024

Abstract
Background
The MYC gene plays a critical role in the diagnosis, prognosis, and treatment of hematological malignancies, including B-cell lymphomas, and both acute myeloid and lymphoid leukemia. The MYC fluorescence in situ hybridization (FISH) break-apart probe (BAP) is used in clinical testing to detect MYC gene rearrangements. Since MYC genomic abnormalities include various breakpoints and more than 35 translocation partner genes, false-negative results could significantly affect patient care. Due to the COVID-19 pandemia, our validated Abbott MYC BAP became unavailable in May 2023 after 20 years of clinical use. To ensure uninterrupted clinical testing, we validated MYC BAPs from three companies using three bone marrow samples with known MYC abnormalities.
Results
Two of the three tested probe sets from three manufacturers (CytoCell, MetaSystems, and Empire Genomics) showed concordant results with Abbott’s BAP for all samples, while one manufacturer’s MYC BAP (Empire Genomics) showed concordance in only 1 of the 3 samples. Using reference loci provided by each company’s probe map, we demonstrated that the discordant results were due to the proximity of the 5’ and 3’ probes, which prevented detection of the known MYC rearrangements, resulting in false-negative results.
Conclusions
Our findings reveal high-risk vulnerabilities in diagnostic testing when presuming equivalency between commercially available MYC BAPs. We recommend that clinical laboratories, in their initial validation process, include probes from multiple sources to account for supply chain disruptions. Additionally, laboratories should carefully compare probe designs when selecting probe manufacturers to ensure consistent and accurate detection of all frequent MYC genomic abnormalities.
WITHDRAWN: Disease modifying effect of HBS1L-MYB in HbE/β-thalassemia patients
Authors: Ferdous Jannatul , Tasnim Marzia , Mannoor Kaiissar , Qadri Firdausi , Hosen Md. Ismail , Shekhar Hossain Uddin 
Published: January 2024

Abstract

Thalassemias are a group of autosomal recessive disorder and the most common inherited disease worldwide with wide geographical variation in incidence. Fetal hemoglobin, hemoglobin is the main oxygen carrier protein in the human fetus. Hemoglobin F is found in fetal red blood cells. Elevated fetal hemoglobin (HbF) level is known to ameliorate the severity of HbE/β and β-thalassemia. This study aimed to investigate whether two commonly known HbF-associated SNPs (rs28384513 & rs4895441) in the HBS1L-MYB region are associated with HbF level and disease severity in Bangladeshi HbE/β- thalassemia patients. Blood samples were collected from a total of 160 participants (120 HbE/β- thalassemia patients and 40 healthy controls). Hematological analysis was performed using complete blood count (CBC) and capillary Hb electrophoresis. After genomic DNA extraction, real-time PCR-based high-resolution melting (HRM) for SNP detection targeting the HBS1L-MYB intergenic region was done. For rs-28384513 SNP the distribution of the major allele ‘TT’ and mutant allele ‘TG + GG’ were found to be 30% and 70%, respectively, while for rs-4895441, 79% of major allele ‘AA’ and 21% of minor allele ‘GG’ were observed in the patients. Patients carrying rs28384513 and rs4895441 SNPs had significantly higher HbF (1.29 ± 1.63 and 1.49 ± 1.7g/dl, respectively) compared to major allele ‘TT’ and ‘AA’ (0.87 ± 1.1 and 1.19 ± 1.65g/dl, respectively) with a p-value of 0.01 and 0.02, respectively. It has been detected that HbF levels in SNP-carrying patients significantly correlated with the higher transfusion interval (60 days, r = 0.38, P < 0.0001) and age of first transfusion (65 months, r = 0.26, p < 0.0028) in these patients. Further, non-transfusion-dependent patients had the highest HbF level (2.03 ± 2.05g/dl) compared to transfusion-dependent moderate (0.58 ± 0.78g/dl) and severe (0.84 ± 1.27g/dl) patients generating a significant p-value < 0.0001 in One-Way ANOVA test. The minor allele frequencies of rs28384513 (G) and rs4895441 (G) were found to be 0.43 and 0.11 respectively in the present study population. These findings suggest that SNPs of HBS1L-MYB may have a role in elevated HbF levels and ameliorating disease severity in terms of transfusion in HbE/β-thalassemia patients.
The Role of Age and AMH On Cumulative Live Birth Rates Over Multiple Frozen-thawed Embryo Transfer Cycles: A Study Based On Low Prognosis Patients Of POSEIDON 3 and 4 Groups
Authors: Xia Lan , Zhou Xiaowei , Wang Xiaoling , Zhao Shen , Wu Xian , Xu Huihui , Zhang Aijun , Niu Zhihong 
Published: January 2024

Abstract

Background: Among the POSEIDON criteria, group 3 and group 4 have an expected low prognosis. For those patients with inadequate ovary reserve, embryo accumulated from consecutive oocyte retrieval cycles for multiple frozen-thawed embryo transfers (FET) has become more common. It is necessary to inform them of the pregnancy outcomes after single or multiple FET cycles before the treatment. However few studies about cumulative live birth rate (CLBR) for those with low prognosis have been reported.
Methods: This retrospective study included 4712 patients undergoing frozen embryo transfer cycles from July 2015 to August 2020. Patients were stratified as POSEIDON group 3, group 4, control 1 group (< 35 years) and control 2 group (≥35 years). The primary outcome is CLBRs up to six FET cycles and the secondary outcomes were LBRs per transfer cycle. Optimistic approach was used for the analysis of CLBRs and the depiction of cumulative incidence curves.
Results: Under optimistic model analyses, control 1 group exhibited the highest CLBR (93.98%, 95%CI 91.63%-95.67%) within 6 FET cycles, followed by the CLBR from women in POSEIDON group 3（92.51%，95%CI 77.1-97.55）was slightly lower than that in control 1 group. The CLBR of POSEIDON group 4（55% ,95%CI 39.34%-70.66%）was the lowest and significantly lower than that of control 2 group（88.7%, 95%CI 80.68%-96.72%）. Further, patients in POSEIDON group 4 reached a CLBR plateau after 5 FET cycles.
Conclusions: The patients of POSEIDON group 3 may not be considered as traditional “low prognosis” in clinical practice as extending the number of FET cycles up to 6 can archive considerably CLBR as control women. While for the POSEIDON group 4, a simple repeat of the FET cycle is not recommended after four failed FET cycles, some strategies such as PGT-A may be beneficial.
Preclinical evaluation of 99m Tc-labeled LHRH as GnRH receptor imaging
Authors: Alfaya Lucía , Camacho Ximena , Cabrera Mirel , Tassano Marcos , Savio Eduardo , Reyes Laura , Paolino Andrea , García María Fernanda , Fernández Marcelo , Gambini Juan Pablo , Cabral Pablo 
Published: January 2024

Abstract
Breast cancer stands as the principal cause of cancer-related mortality among women in the developed world. Notably, receptors of Luteinizing Hormone-Releasing Hormone (LHRH or GnRH) exhibit overexpression in this malignancy. This study aimed to develop a new molecular prove [99mTc] Tc-HYNIC-GSG-LHRH(D-Lys6)/Tricine/Nicotinic Acid (NA) as a novel molecular imaging agent for breast cancer. HYNIC-GSG-LHRH(D-Lys6) was acquired and radiolabeled with [99mTc] Tc. The radiochemical purity and stability in different conditions were evaluated by Instant thin-layer chromatography (ITLC) and High performance liquid chromatography (HPLC). Lipophilicity was performed by the distribution coefficient test. In vitro cell binding studies were performed in different human and mice breast cancer cell lines (MDA-MB-231, MDA-MB-435, MCF-7, BT474 and 4T1) as well as in normal murine fibroblasts (NIH-3T3) and CHO-K1 as negative control. Biodistribution studies were performed in normal Balb/c mice and 4T1 tumor-bearing Balb/c mice up to 6 h post-injection. SPECT/CT images were performed in 4T1 tumor-bearing Balb/c mice up to 5 h post injection (p.i). [99mTc] Tc-HYNIC-GSG-LHRH(D-Lys6)/Tricine/NA complex was labeled with a high radiochemical purity (> 98%) and stable up to 4 hs. It presented a good hydrophilicity (Log P = − 2.82 ± 0.04). It also yields a relevant and specific binding in all breast cell lines evaluated. Biodistributions studies showed a high renal clearance and low unspecific binding (< 2% Act/g) in most organs, as well as appreciable tumor uptake (5.8 ± 0.5%ID/g 1 h p.i) and high tumor/muscle ratio (maximum of 30.5 ± 11.2 at 1 h p.i). SPECT/CT of 4T1-tumor bearing Balb/c mice images revealed similar results to biodistribution studies, with a Tumor/Non-Tumor ration of > 3.5 at all times evaluated. In vivo blockage studies showed specificity for the LHRH-R; demonstrating a substantial potential for in vivo visualization of LHRH-R expression in breast cancer.
“I have to breathe”: A mixed-methods pilot study to assess the feasibility, acceptability, cost, and effectiveness of an intervention for post-tuberculosis lung disease
Authors: Almeida Carlos , Durand Rafael , LaHood Allison , Byrne Anthony , Mendoza-Ticona Alberto , Ygnacio Erika , Durand Fernando , Lecca Leonid , Pita Ramón , Guerra Dalia , Wong Milagros , Tintaya Karen , Mitnick Carole 
Published: January 2024

Abstract
Background
Many tuberculosis patients who are successfully treated according to microbiologic endpoints experience sequelae that adversely affect quality of life and increase mortality. Prior research in Peru revealed an important burden of respiratory symptoms after successful treatment of tuberculosis. Here, we present a pilot intervention of 8 weeks of pulmonary rehabilitation and inhaled pharmacotherapy for people with respiratory symptoms after successful tuberculosis treatment. We examine the feasibility, acceptability, and cost of the intervention, “Libre Post-TB”.
Methods
This was a mixed methods study conducted in Lima, Peru. We recruited adults with respiratory symptoms within three months of successful completion of TB treatment. At baseline, we recorded demographic characteristics, vital signs, medical history, and chest X-ray findings. At baseline and end of study, we evaluated respiratory symptoms, quality of life, lung function, fitness, strength, and fatigue. We surveyed participants on direct medical, non-medical and indirect costs of participation in the intervention and estimated its cost in the public health system. We assessed feasibility with senior health system personnel (N = 4) and primary care workers (N = 10) and acceptability with intervention participants (N = 14). We report the number of pulmonary rehabilitation sessions completed and integrated these results with those from the qualitative acceptability component.
Results
We enrolled 40 participants. Spirometry-defined COPD was detected in 3 (7.5%). In 10 (25%) and 9 (22.5%) participants, FEV1 and FVC, respectively, was below age-, sex-, height- standardized lower limits of normal. Participants completed 11 (IQR: 5,15) of 16 pulmonary rehabilitation sessions. Participants generally appreciated the virtual intervention and associated flexibility; for some this was inadequate to guarantee completion. Differences in lung function were not apparent while improvement in quality-of-life was detected after intervention. Some providers normalized post-TB symptoms. Feasibility findings included that limited guidance and facilities for post TB lung disease should not preclude its implementation.
Conclusions
A brief pulmonary rehabilitation intervention was feasible and acceptable for people with respiratory symptoms following successful TB treatment in Peru. There is some evidence for improved symptomatology. With an estimated 155 million TB survivors, and high prevalence of PTLD, the potential impact of improved interventions is enormous.
WITHDRAWN: Optimizing Brain Tumor Classification: A Comparative Analysis of Nature Inspired Algorithms with GLCM Features
Authors: P selvan , A Kavitha 
Published: January 2024

Abstract

Advancements in medical imaging have led to an increasing demand for accurate and efficient methods of brain tumor classification. This study delves into the realm of nature-inspired optimization algorithms, with a focus on their application in the field of medical image analysis. We examine the performance of three distinct algorithms: Firefly, Cat Swarm Optimization (CSO), and Artificial Fish Swarm Optimization Algorithm (AFSA), in the context of brain tumor classification. Among these, CSO emerges as the star performer, achieving an impressive accuracy rate of 96.36%. The study employs Gray-Level Co-occurrence Matrix (GLCM) features, a widely recognized set of texture features for medical image analysis. Through a rigorous comparative analysis, we explore the capabilities of these algorithms in accurately classifying brain tumors, shedding light on their potential to enhance diagnostic precision.
Prenatal detection of chromosome 7q deletion with duplication and literature review
Authors: Zhu Jinping , Hu Juan 
Published: January 2024

Abstract
Background
Genetic analysis was performed on a fetus with partial deletion and duplication of 7q, proposed by non-invasive prenatal screening (NIPT) as a reference for clinical genetic counseling.
Case presentation
Pregnant woman, Non-invasive prenatal screening suggested a 7.8 Mb deletion of chromosome 7 q36.1q36.3 and a duplication of 9.3 Mb in the region of 7q34q36.1. Conventional chromosome G-banding and chromosome microarray analysis(CMA) were performed on fetal amniotic fluid samples and parental peripheral blood samples. The fetal karyotype was 46, XY on conventional G-banding analysis. The CMA test results showed a deletion of approximately 7.8 Mb in the 7q36.1q36.3 region and a duplication of 6.6 Mb in the 7q35q36.1 region. The parents' karyotype analysis and CMA results were normal, indicating a new mutation.
Conclusion
CMA molecular diagnostic analysis can effectively detect chromosomal microdeletions or microduplications, clarify the relationship between fetal genotype and clinical phenotype, and provide a reference for prenatal diagnosis of chromosomal microdeletion-duplication syndrome.