bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing the 10-year Persian Adult Hypertension Risk Score (PAHRS) Based on Yazd YHHC Healthy Heart Cohort (YHHC) Study: A Longitudinal Study on 20-74 Year-Old Population of Yazd
Authors: Namayandeh Seyedeh Mahdieh , MohammadiMoghaddam Hossein , Fallahzadeh Hossein , Majidpoure Fatemeh , Soltani Mohammadhosein , SadrBafghi Seyed Mahmood , Ghomi Farnoosh 
Published: January 2024

Abstract

Introduction
Hypertension is major risk factor for cardiovascular and renal diseases and has become one of the major global challenges.To Development of a 10-year Persian Adult Hypertension Risk Score (PAHRS) based on Yazd healthy heart cohort study data. The present study is a longitudinal study as a sub study of Yazd Healthy Heart Cohort (YHHC).
Method
A total number of 1152 out of 2000 participants aged 20–74 years without hypertension at baseline were included in this study. The risk scoring system was used to predict the incidence of hypertension over a ten-year follow-up using logistic regression model. The performance of the predictive algorithm was evaluated using calibration and model discrimination. A web application was developed based on this study result and risk assessment system with link of https://survey.porsline.ir/s/2OGlwx6H.
Result
A total number of 324 patients (36.3%, mean age of 42.8 years) became hypertensive at the end of the 10-year follow up of the present study. In the multivariate logistic regression model, age, family history of hypertension, baseline systolic hypertension, history of diabetes, hyperglycemia, and obesity were identified as independent predictors of hypertension. Participants according to their ten-years hypertension risk by the point score system classified into three groups: low-risk, with a risk of < 10% in 135 participant (11.7%) and intermediate-risk,with a mean risk of 10% -20% in 319 participant (27.7%) and high-risk with a risk of > 20% in 698 participant(60.6% ). The value of C statistic for the point score system was calculated to be 0.72 and the Hosmer-Lemeshow test for the calibration was acceptable (χ2 = 3.26, P = 0.917) for ten years of incidence of hypertension in the YHHC study.
Conclusion
The Persian Adult Hypertension Risk Score (PAHRS), which was developed for the first time using Yazd native residents' data, is a simple and useful tool in hypertension risk assessment and consider prevention program based on high risk population approach.. The almost two third of the our study population was estimated to be at high risk for hypertension during ten years so using our risk scoring system can be used for health policy in primary prevention of hypertension in communities.
Evaluation of the effect of the Azadirachta indica (Margosa) leaf syrup on the control of the caterpillar in cabbage in the community of Phandira, Dunda, District of Macossa in Republic of Mozambique.
Authors: Parwez Zahid , Joaquim Boneze Nhampoca Jaime 
Published: January 2024

Abstract

A descriptive experimental study was carried out to have a bird eye view on the effect of the syrup made from leaves of Azadirachta (Margosa) indica A. Juss (Neem) on the control of the caterpillar (Trichoplusia ni) in cabbage seedlings, and the plot method was applied to the samples of 250 seedlings. The trial was carried out between January and May 2022 and a completely casualized block design (DBCC) was used, with 4 treatments, making a total of 15 experimental units (plots). The area was divided into 3 randomized blocks and each block with 5 plots where the sowing was carried out in lines with a 15 cm compass between lines in the same plot and the seeds were released within each line, without having a space between the seeds within the lines once dealing with a nest. Three parameters were evaluated, Population density, Infestation level and Medium attack level. The collected data were submitted to statistical processing using (SISVAR 5.6) and analysis of variance and Tukey's test at 5% of significance, however, significant differences were found for the parameters degree of infestation (GI) and number of caterpillars (NL) related to the percentage of caterpillar mortality when submitted to different doses of Neem extract application.
A bilateral four-headed brachialis muscle with variant innervation: a cadaveric report with possible clinical implications
Authors: PIAGKOU MARIA , Triantafyllou George , Koutsougeras Aggelos , Koutserimpas Christos , Katsogiannis Dimitris , Georgiev Georgi , Olewnik Lukasz , Zielinska Nicol , Tsakotos George 
Published: January 2024

Abstract
Purpose: Anterior compartment muscles of the arm present high morphological variability, with possible clinical significance. The current cadaveric report aims to describe a bilateral four-headed brachialis muscle (BM) with aberrant innervation. Analysis of the embryological background and possible clinical significance of the BM variant is also provided.
Methods: Classical dissection of the upper limb was performed on an 84-year-old donated male cadaver. The cadaver belonged to the Anatomy Department of the National and Kapodistrian University of Athens.
Results: On the left upper limb, the four-headed BM was supplied by the musculocutaneous and the median nerves after their interconnection. On the right upper limb, the four-headed BM received its innervation from the median nerve due to the musculocutaneous nerve absence. A bilateral muscular tunnel for the radial nerve passage was identified, between the BM accessory heads and the brachioradialis muscle.
Conclusion: BM has clinical significance, due to its proximity to important neurovascular structures and frequent surgeries at the humerus. Hence, knowledge of these variants should keep orthopedic surgeons alert when intervening in this area.
Determination of Perfluorinated Substances (Pfas) in Certain Foodstuffs According to Newly Established Eu Legislation
Authors: Pasecnaja Elina , Zacs Dzintars 
Published: January 2024

Abstract
Per- and polyfluorinated alkyl substances (PFAS) are compounds which exhibit unique chemical and physical properties resulting in bioaccumulation in aquatic and terrestrial food chains. Due to a global concern on the adverse health effects, European Food Safety Authority (EFSA) set tolerable weekly intake and thereafter, to ensure an efficient protection of public health, the European Commission set maximum levels for four priority components, namely perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorohexanesulfonic acid (PFHxS), and perfluorooctanesulfonic acid (PFOS), in certain foodstuffs. This study demonstrates an analytical method based on high performance liquid chromatography (HPLC) coupled to Orbitrap mass spectrometry (Orbitrap-MS) for the quantitative determination of four priority PFAS. The optimized and validated LC-Orbitrap-MS method fulfils the requirements specified in the “Guidance Document on Analytical Parameters for the Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Food and Feed” and allows a reliable analysis of PFAS in selected food products, fulfilling the requirements of Commission Regulation 915/2023, Commission Regulation (EU) 2022/1428, and Commission Recommendation (EU) 2022/1431. The method was successfully used for the compliance testing of four priority PFAS according to the newly established legislation in food samples (n=58) collected in Latvia, providing the occurrence data from the Baltic states.
Systematic review of the feasibility to spare the rectoprostatic fascia in male patients undergoing total mesorectal excision for rectal cancer
Authors: Nikolouzakis Taxiarchis , Gouvas Nikolaos , Athanasakis Elias , Mariolis-Sapsakos Theodoros , Chrysos Emmanouel , Tsiaoussis John 
Published: January 2024

Abstract
Background
The main purpose of this study was to identify feasibility to spare rectoprostatic fascia (RPF) in adult male cadavers and in adult male patients undergoing total mesorectal excision (TME) for rectal cancer. A secondary objective was to evaluate urogenital function following rectal cancer surgery and oncologic outcome.
Methods
Adhering to PRISMA guidelines, we performed a systematic review with a priori design to identify relevant studies using MESH terms and keywords. Research was conducted through 1st of March to 31st of July 2023 in MEDLINE, SCOPUS, Web of Science, Google Scholar and reference lists. Disagreements between individual judgments were resolved with referral to a third researcher.
Results
In total 1211 studies were identified out of which 17 were relevant to the main research question. Overall, 496 living individuals and 25 cadavers were included in whom RPF was identified and spared.
Conclusions
Despite the fact that current practice on rectal cancer surgery dictates TME to extend anterior to the RPF, based on our findings, RPF-spare TME is not only feasible but also important for preserving fair male urogenital function especially for young patients whose erectile function is a priority.
Trial registration number and date of registration:
CRD42020171188, February 2023
Prevalence of Poor Glycemic Control and Associated Factors among Adult Type 2 Diabetes Mellitus Patient at ALERT Hospital, Addis Ababa, Ethiopia, 2022
Authors: Mohammed Fuad Ahmed , Ali Aziza Abdella , Taye Tehetena Assefa 
Published: January 2024

Abstract
Background
Over the last fifty years, type 2 diabetes have become a significant contributor to the global disease burden, and this trend is expected to continue. The Diabetes Prevention Program and the Finnish Diabetes Prevention Study showed the value of primary diabetes prevention, and many studies emphasized the significance of strict management of plasma glucose levels in reducing complications in both type 1 and type 2 diabetes. The aim of this observational study was to assess the prevalence and the associated factors for glycemic control among adult type 2 DM patients at ALERT hospital, Addis Ababa, Ethiopia, 2022.
Method
An institutional based cross-sectional study was employed The study population was adult type 2 DM patients with or without other comorbidities who were on follow at follow up clinic at ALERT hospital. Using a single population proportion formula the final sample size for this study was 371. A systematic random sampling technique was used to select a sample size of 174 pregnant women. The data was collected by trained data collectors being under supervision by the principal investigator. Then, it was cleaned, entered and analyzed using SPSS version 26.0 statistical software and MS excel.
Result
From the 371 participants, 60.9% were male participants; 55.3% between 35 and 64 years of age and 49.1% of them were married. Overall prevalence of poor glycemic control was 89.5%. associated hypertension (p-value = 0.049, AOR = 5.850) was significantly associated with poor glycemic control. having history of hospital admission (p-value = 0.028, AOR = 0.212), recent Fast Blood Sugar of 70-130mg/dl (p-value = 0.003, AOR = 0.025) and access for Self Monitoring of Blood Glucose p-value = 0.021, AOR = 0.046) were negatively associated with poor glycemic control.
Conclusion and Recommendations:
The proportion of poor glycemic control among the type 2 diabetic patients was very high in this study. Educating and preparing methods on managing glycemic control and associated factors for diabetic patients.
Effects of high-molecular-weight glutenin subunit on hard-steamed bread quality
Authors: Zhao Jing , Wang Tianyi , Kai Mengxiang , Zhao Jiajia , Qiao Ling , Wu Bangbang , Hao Yuqiong , Ge Chuan , Wang Juanling , Feng Zhiwei , zheng Jun 
Published: January 2024

Abstract
Steamed bread is used as a daily food in many countries worldwide, but the relationship between high-molecular-weight glutenin (HMW-GS) and steamed bread quality is unclear. Two DH populations of common wheat were used to analyze the effects of twelve HMW-GS subunit combinations on the quality traits of steamed bread, including volume traits, physical properties, and sensory evaluation. The mechanism of trait variation was analyzed by combining the microstructure and dough rheological properties. The results showed that in terms of locus effect, the volume and physical properties of steamed bread were Glu-D1> Glu-B1> Glu-A1, and the effect of sensory score on steamed bun was Glu-B1>Glu-D1>Glu-A1. In terms of individual subunit effects, the volume, chewiness, hardness and so on physical properties indexes and sensory scores of subunit 1 steamed bread at Glu-A1locus were higher than those of subunit N, but the number of cells in subunit N steamed bread was more than subunit 1, as well as the cell diameter and wall thickness were smaller than those of subunit 1 steamed bread. The quality of 7+8 and 7+9 subunits steamed bread at the Glu-B1locus was generally better, and the chewiness, number of cells and so on physical properties indexes and sensory scores were significantly higher than those of 6+8 subunit steamed bread, as well as their physical properties indexes were less affected by moisture. The quality of subunits 2+10 and 5+12 steamed bread at the Glu-D1locus was generally better. Moreover, the specific volume, glueyness, number of cells and so on physical properties were significantly better than those of other subunit steamed bread, as well as the physical properties of subunits 2+10, 5+12 and 2+12 were also less affected by moisture. The effect of subunit combination is mainly affected by subunit, and the combination of superior subunits tends to make steamed bread have better quality traits. The subunit combinations (1,7+8,5+12), (N,7+9,2+10) and (1,7+9,5+12) had better physical properties indexes and sensory scores, and the micro-pore structure was dense, uniform and delicate, and the wall thickness was smaller. However, the combinations (N,7+8,2+12), (N,6+8,2+12) and (N,7+9,5+10) were generally lower in physical properties and sensory scores, and the stomatal structure was looser. The results of different subunits of dough rheological properties showed that the protein content, wet gluten content and stability time were the main factors affecting steamed bread's volume and physical properties. Among the rheological properties of dough, the protein content, wet gluten content and stability time of subunits 7+8, 7+9, 2+10 and 5+12 were higher. Therefore, the volume, physical properties and sensory scores of steamed bread containing these subunits were good.
Controllable Preparation of Hydrangea Shaped CoAl-LDHs@CoF2 Composite for Supercapacitor Electrode with Superior Performance
Authors: Bai Xue , Sun Fengyi , Ma LiYan , Shen Jyunhong , Jiang Zhuwu , Xu Dongdong , Pan Chuntao , Di Hongcheng , Zhang Hongyu 
Published: January 2024

Abstract

Exploring the method for rational design hierarchical structures such as hollow, sphere and core-shell structure is a crucial challenge for enhancing electrochemical performance of supercapacitor electrodes. In this study, we demonstrate a facile method for controllable preparation of hydrangea shaped CoAl-LDHs@CoF2 composite and used as positive electrode for supercapacitor. A series of contrast tests are performed to select optimal experiment condition. CoAl-LDHs obtained at metal ratio of 2:1, reacting 8 h with addition of NH4F, displays optimal performance associated with the hierarchical structure, where NH4F plays a vital role in regulating this uniform generation process. The fabricated working electrode shows high specific capacitance of 827.8 Fg− 1, with excellent capacitance retention of 62.8% after current density increases ten times. At last, an asymmetric supercapacitor using C2A1-8h sample as positive electrode and activated carbon as negative electrode, is assembled and delivers an outstanding energy density of 53.7 Wh kg− 1 at power density of 239.9 kW kg− 1, and energy density reaches 27.8 Wh kg− 1 at maximum power density of 12000 W kg− 1, indicating C2A1-8h is a potential candidate for energy storage and conversion systems.
A comparison of anterior reconstruction of spinal defect using nano-hydroxyapatite/polyamide 66 cage and autologous iliac bone for thoracolumbar tuberculosis: a stepwise propensity score matching analysis
Authors: Li Qiujiang , Xiu Peng , Yang Xi , Wang Lei , Liu Limin , Song Yueming 
Published: January 2024

Abstract
Purpose
Previous studies have confirmed the advantages and disadvantages of autogenous iliac bone and nano-hydroxyapatite/polyamide 66 (n-HA/PA66) cage. However, there is no conclusive comparison between the efficacy of the two implant materials in spinal tuberculosis bone graft fusion. The aim of this study was to analyze the mid- to long-term clinical and radiologic outcomes between n-HA/PA66 cage and autogenous iliac bone for anterior reconstruction application of spinal defect for thoracolumbar tuberculosis.
Methods
We retrospectively reviewed all patients who underwent anterior debridement and strut graft with n-HA/PA66 cage or iliac bone combined with anterior instrumentations between June 2009 and June 2014. One-to-one nearest-neighbor propensity score matching (PSM) was used to match patients who underwent n-HA/PA66 cage to those who underwent iliac bone. Clinical outcomes were assessed using the Japanese Orthopaedic Association (JOA) and visual analogue score (VAS). Radiographic evaluations included cage subsidence and segmental angle.
Results
At the end of the PSM analysis, 16 patients from n-HA/PA66 cage group were matched to 16 patients in Iliac bone group. The C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) values in the n‑HA/PA66 group decreased significantly from 33.19 ± 10.89 and 46.63 ± 15.65 preoperatively, to 6.56 ± 2.48 and 9.31 ± 3.34 at the final follow‑up, respectively (P < 0.001). There were no significant differences in the CRP and ESR values between the two groups at the final follow‑up. The VAS and JOA scores in the iliac bone and n‑HA/PA66 group were significantly improved at the 3-month follow-up postoperatively (both P < 0.001). Then, improvements of VAS and JOA scores continue long at final follow-up. However, there were no significant differences in the VAS and JOA scores at any time point between the two groups (P > 0.05). Although the SA significantly increased after surgery in both groups, there was no significant difference at any time point after surgery (P > 0.05). There were no significant differences in the cage subsidence and fusion time between the two groups.
Conclusion
Overall, our data suggest that the n-HA/PA66 cage outcomes are comparable to those in the autogenous iliac bone, with a similar high fusion rate as autogenous iliac bone.
Enhancing Vernier Effect through Integrated Add-Drop Microresonator for High Sensitivity All-Optical Sensing
Authors: Azrisham Nurul Fathinah , Aman Mohammad Amirul Hairol , Noorden Ahmad Fakhrurrazi Ahmad , Bahadoran Mahdi 
Published: January 2024

Abstract
The Vernier effect has recently sparked a lot of interest study in high sensitivity sensing due to its ability to suppress interstitial peaks and broaden the Free Spectral Range (FSR). An analytical simulation system was constructed to incorporate an add-drop Micro Ring Resonator (MRR) waveguide with partial reflecting air holes into the bus waveguide to acquire the interferometric resonance and generate the Vernier effect. The system was developed based on the scattering matrix method and the Optical Transfer Function (OTF). A comparison analysis has been conducted between Conventional Add-Drop (CAD) MRR and Interferometric Add-Drop (IAD) MRR configurations to measure the improved sensitivity. The spectrum was analyzed based on the FSR and the wavelength shift by the change of the refractive index. Both CAD and IAD-MRR have been optimized by varying the radii of the ring and the distance between the air holes in the bus waveguide. The optimized IAD configuration demonstrates a 166.11% enhancement in FSR value and yields higher sensitivity by 17.07% via the implementation of the reflecting holes in the bus waveguide. The IAD-MRR configuration also generated a higher Q factor value of more than four times than the CAD-MRR. These findings emphasize the potential of interferometric resonance in enhancing the Vernier effect thus achieving better performance of all-optical sensing applications.
Knockout of the bread wheat CER9/SUD1 gene using CRISPR/Cas technology
Authors: Musin Khalit , Mikhaylova Elena , Galimova Aizilya , Baimukhametova Elvina , Zaikina Evgenia , Kuluev Azat , Ibragimova Zarina , Rakhmatullina Irina , Berezhneva Zoya , Kuluev Bulat 
Published: January 2024

Abstract

Global warming has underscored the imperative of drought tolerance as a paramount trait in bread wheat. Augmenting the cuticular wax layer on leaves and stems stands as one viable approach to enhancing plant drought tolerance. A pivotal discovery surfaced by identifying a mutation within the CER9 gene of Arabidopsis thaliana, amplifying the cuticular wax and consequently mitigating water loss, thereby fortifying drought resilience. Notably, analogous genes, termed SUD1, have been annotated in bread wheat, albeit lacking in-depth scrutiny regarding their phenotypic manifestations under the drought. Hence, our study aimed to employ CRISPR/Cas technology to knockout the CER9/SUD1 gene in bread wheat. For this, five guide RNAs were meticulously chosen and merged into a singular vector. Delivery of the CRISPR/Cas components were arranged through Agrobacterium tumefaciens, utilized for transforming immature embryos of two agricultural spring bread wheat varieties: Taya and Sigma. Among the 13 transgenic plants procured, four manifested positivity for the reporter gene GFP and Cas9 gene. Notably, substantial deletions ranging from 284 bp to 398 bp within the CER9/SUD1 gene were discerned in these plants. Additionally, two of the edited plants exhibited an absence of CER9/SUD1 transcripts, while the other two displayed a noteworthy 5.4-fold reduction in CER9/SUD1 gene expression compared to the wild type. Intriguingly, the genome-edited plants of the T1 generation showcased enhanced growth compared to the wild type under both standard and drought conditions.
Molecular Characterization of HPV Integrations in High-risk HPV- positive Women and Constructing an Integration Gene-Based Prognostic Risk Model for Cervical Cancer
Authors: Lyu Qiongying , Chen Yurou , Xiong Jiaqiang , Zhang Juan , He Xiaoyan , Wang Hairong , Wang Lihan , Zhang Wei 
Published: January 2024

Abstract
Background
Cervical cancer (CC) ranks fourth in global cancer incidence and represents the second leading cause of cancer deaths. HPV integration into the human genome is a key molecular event in the progression of cancer carcinogenesis.
Methods
This study utilized the high-risk HPV-positive women samples to investigate the HPV integration through the high-throughput Viral Integration Detection (HIVID). Subsequently, the expression of recurrent integration genes at both the mRNA and protein level was determined in GEIPA, and the Human Protein Atlas (HPA). Furthermore, the prognostic risk model of HPV integration genes was constructed using the univariate Cox, least absolute shrinkage and selection operator (LASSO) regression and multivariate Cox analysis from the Cancer Genome Atlas (TCGA) datasets. Then, the prognostic risk model was evaluated with Kaplan-Meier (KM) survival curves and receiver operating characteristic (ROC) curves. The CC patients in TCGA were stratified into high and low risk groups and the differences in PI3K-AKT pathway gene enrichment and immunotherapeutic response differences were analyzed between the two subgroups. Finally, the deferentially expressed immune-related genes (immune DEGs) between the high-risk and low-risk groups were subjected to functional enrichment analysis.
Results
The overall HPV integration rate in high-risk HPV-positive women was 8.75%. We identified 467 integration sites in 82 out of 937 samples. We detected 28 recurrent genes for virus integration in 318 integration genes (reads > = 6), the most frequent were KLF5 (n = 5), LINC00392 (n = 5), BCL11B (n = 3) and TP63 (n = 3).