bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs
Authors: He Ye , Yang Shujie , Liu Pengzhan , Li Ke , Jin Ke , Becker Ryan , Zhang Jinxin , Lin Chuanchuan , Xia Jianping , Ma Zhehan , Ma Zhiteng , Zhong Ruoyu , Lee Luke P. , Huang Tony Jun 
Published: November 2023

AbstractWhile mesenchymal stem cells (MSCs) have gained enormous attention due to their unique properties of self-renewal, colony formation, and differentiation potential, the MSC secretome has become attractive due to its roles in immunomodulation, anti-inflammatory activity, angiogenesis, and anti-apoptosis. However, the precise stimulation and efficient production of the MSC secretome for therapeutic applications are challenging problems to solve. Here, we report on Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs: AIMS. We create an acoustofluidic mechanobiological environment to form reproducible three-dimensional MSC aggregates, which produce the MSC secretome with high efficiency. We confirm the increased MSC secretome is due to improved cell-cell interactions using AIMS: the key mediator N-cadherin was up-regulated while functional blocking of N-cadherin resulted in no enhancement of the secretome. After being primed by IFN-γ, the secretome profile of the MSC aggregates contains more anti-inflammatory cytokines and can be used to inhibit the pro-inflammatory response of M1 phenotype macrophages, suppress T cell activation, and support B cell functions. As such, the MSC secretome can be modified for personalized secretome-based therapies. AIMS acts as a powerful tool for improving the MSC secretome and precisely tuning the secretory profile to develop new treatments in translational medicine.
Enhancing the sensitivity of atom-interferometric inertial sensors using robust control
Authors: Saywell Jack C. , Carey Max S. , Light Philip S. , Szigeti Stuart S. , Milne Alistair R. , Gill Karandeep S. , Goh Matthew L. , Perunicic Viktor S. , Wilson Nathanial M. , Macrae Calum D. , Rischka Alexander , Everitt Patrick J. , Robins Nicholas P. , Anderson Russell P. , Hush Michael R. , Biercuk Michael J. 
Published: November 2023

AbstractAtom-interferometric quantum sensors could revolutionize navigation, civil engineering, and Earth observation. However, operation in real-world environments is challenging due to external interference, platform noise, and constraints on size, weight, and power. Here we experimentally demonstrate that tailored light pulses designed using robust control techniques mitigate significant error sources in an atom-interferometric accelerometer. To mimic the effect of unpredictable lateral platform motion, we apply laser-intensity noise that varies up to 20% from pulse-to-pulse. Our robust control solution maintains performant sensing, while the utility of conventional pulses collapses. By measuring local gravity, we show that our robust pulses preserve interferometer scale factor and improve measurement precision by 10× in the presence of this noise. We further validate these enhancements by measuring applied accelerations over a 200 μg range up to 21× more precisely at the highest applied noise level. Our demonstration provides a pathway to improved atom-interferometric inertial sensing in real-world settings.
The heat shock protein LarA activates the Lon protease in response to proteotoxic stress
Authors: Omnus Deike J. , Fink Matthias J. , Kallazhi Aswathy , Xandri Zaragoza Maria , Leppert Axel , Landreh Michael , Jonas Kristina 
Published: November 2023

AbstractThe Lon protease is a highly conserved protein degradation machine that has critical regulatory and protein quality control functions in cells from the three domains of life. Here, we report the discovery of a α-proteobacterial heat shock protein, LarA, that functions as a dedicated Lon regulator. We show that LarA accumulates at the onset of proteotoxic stress and allosterically activates Lon-catalysed degradation of a large group of substrates through a five amino acid sequence at its C-terminus. Further, we find that high levels of LarA cause growth inhibition in a Lon-dependent manner and that Lon-mediated degradation of LarA itself ensures low LarA levels in the absence of stress. We suggest that the temporal LarA-dependent activation of Lon helps to meet an increased proteolysis demand in response to protein unfolding stress. Our study defines a regulatory interaction of a conserved protease with a heat shock protein, serving as a paradigm of how protease activity can be tuned under changing environmental conditions.
A study of the wave dynamics of the space–time fractional nonlinear evolution equations of beta derivative using the improved Bernoulli sub-equation function approach
Authors: Podder Anamika , Arefin Mohammad Asif , Akbar M. Ali , Uddin M. Hafiz 
Published: November 2023

AbstractThe space–time fractional nonlinear Klein-Gordon and modified regularized long-wave equations explain the dynamics of spinless ions and relativistic electrons in atom theory, long-wave dynamics in the ocean, like tsunamis and tidal waves, shallow water waves in coastal sea areas, and also modeling several nonlinear optical phenomena. In this study, the improved Bernoulli sub-equation function method has been used to generate some new and more universal closed-form traveling wave solutions of those equations in the sense of beta-derivative. Using the fractional complex wave transformation, the equations are converted into nonlinear differential equations. The achieved outcomes are further inclusive of successfully dealing with the aforementioned models. Some projecting solitons waveforms, including, kink, singular soliton, bell shape, anti-bell shape, and other types of solutions are displayed through a three-dimensional plotline, a plot of contour, and a 2D plot for definite parametric values. It is significant to note that all obtained solutions are verified as accurate by substituting the original equation in each case using the computational software, Maple. Additionally, the results have been compared with other existing results in the literature to show their uniqueness. The proposed technique is effective, computationally attractive, and trustworthy to establish more generalized wave solutions.
Morphological, histological and gene-expression analyses on stolonization in the Japanese Green Syllid, Megasyllis nipponica (Annelida, Syllidae)
Authors: Nakamura Mayuko , Oguchi Kohei , Sato Daisuke S. , Kato Sumika , Okanishi Masanori , Hayashi Yoshinobu , Aguado M. Teresa , Miura Toru 
Published: November 2023

AbstractBenthic annelids belonging to the family Syllidae (Annelida, Errantia, Phyllodocida) exhibit a unique reproduction mode called “schizogamy” or “stolonization”, in which the posterior body part filled with gametes detaches from the original body, as a reproductive unit (stolon) that autonomously swims and spawns. In this study, morphological and histological observations on the developmental processes during stolonization were carried out in Megasyllis nipponica. Results suggest that the stolon formation started with maturation of gonads, followed by the formation of a head ganglion in the anteriormost segment of the developing stolon. Then, the detailed stolon-specific structures such as stolon eyes and notochaetae were formed. Furthermore, expression profiles of genes involved in the anterior–posterior identity (Hox genes), head determination, germ-line, and hormone regulation were compared between anterior and posterior body parts during the stolonization process. The results reveal that, in the posterior body part, genes for gonadal development were up-regulated, followed by hormone-related genes and head-determination genes. Unexpectedly, Hox genes known to identify body parts along the anterior–posterior axis showed no significant temporal expression changes. These findings suggest that during stolonization, gonad development induces the head formation of a stolon, without up-regulation of anterior Hox genes.
Prediction of blood pressure changes during surgical incision using the minimum evoked current of vascular stiffness value under sevoflurane anesthesia
Authors: Shorin Daiki , Kamiya Satoshi , Nakamura Ryuji , Ishibashi Ayaka , Saeki Noboru , Tsuji Toshio , Tsutsumi Yasuo M. 
Published: November 2023

AbstractNecessary and sufficient opioids should be administered for safe and stable anesthesia. However, opioid sensitivity varies among individuals. We previously reported that sympathetic responses to nociceptive stimuli under propofol anesthesia could be predicted by measuring the minimum evoked current of the vascular stiffness value (MECK). However, this result has only been proven under propofol anesthesia. We propose that MECK could be used under anesthesia with a volatile anesthetic. Thirty patients undergoing laparotomy with sevoflurane anesthesia received 0.7 minimum alveolar concentration (MAC) sevoflurane and intravenous remifentanil at a constant concentration of 2 ng/mL, followed by tetanic stimulation, to measure MECK. After tetanic stimulation, the same anesthetic conditions were maintained, and the rate of change in systolic blood pressure (ROCBP) during the skin incision was measured. The correlation coefficient between the MECK and ROCBP during skin incision under sevoflurane anesthesia was R =  − 0.735 (P < 0.01), similar to that in a previous study with propofol (R =  − 0.723). Thus, a high correlation was observed. The slope of the linear regression equation was − 0.27, similar to that obtained in the study on propofol (− 0.28). These results suggest that, as with propofol anesthesia, MECK can be used as a predictive index for ROCBP under 0.7 MAC sevoflurane anesthesia.Clinical trial registration: Registry, University hospital Medical Information Network; registration number, UMIN000047425; principal investigator’s name, Noboru Saeki; date of registration, April 8, 2022.
TREYESCAN: configuration of an eye tracking test for the measurement of compensatory eye movements in patients with visual field defects
Authors: Faraji Yasmin , van Rijn Joris W. , van Nispen Ruth M. A. , van Rens Ger H. M. B. , Melis-Dankers Bart J. M. , Koopman Jan , van Rijn Laurentius J. 
Published: November 2023

AbstractThe Traffic Eye Scanning and Compensation Analyzer (TREYESCAN) is introduced as an innovative eye tracking test designed to measure compensatory eye movements in individuals with visual field defects. The primary objective of the test is to quantitatively assess and analyze the compensatory eye movements employed by patients with visual field defects while viewing videos of various traffic scenes from the viewpoint of a driver of a passenger car. The filming process involved capturing a wide range of driving conditions and hazards, aiming to replicate real-world scenarios. Specific dynamic areas of interest within these scenes were selected and assessed by a panel of experts on medical and practical fitness to drive. Pilot measurements were conducted on a sample of 20 normally-sighted individuals during two different measurement sessions. The results provide valuable insights into how individuals without visual impairment view the dynamic scenes presented in the test. Moving forward, the TREYESCAN will be used in a case–control study involving glaucoma patients and control subjects, with the goal of further investigating and understanding the mechanisms employed by individuals with glaucoma to compensate for their visual field defects.
Plant-based production of a protective vaccine antigen against the bovine parasitic nematode Ostertagia ostertagi
Authors: Zwanenburg Laurens , Borloo Jimmy , Decorte Bregt , Bunte Myrna J. M. , Mokhtari Sanaz , Serna Sonia , Reichardt Niels-C. , Seys Leen J. M. , van Diepen Angela , Schots Arjen , Wilbers Ruud H. P. , Hokke Cornelis H. , Claerebout Edwin , Geldhof Peter 
Published: November 2023

AbstractThe development of effective recombinant vaccines against parasitic nematodes has been challenging and so far mostly unsuccessful. This has also been the case for Ostertagia ostertagi, an economically important abomasal nematode in cattle, applying recombinant versions of the protective native activation-associated secreted proteins (ASP). To gain insight in key elements required to trigger a protective immune response, the protein structure and N-glycosylation of the native ASP and a non-protective Pichia pastoris recombinant ASP were compared. Both antigens had a highly comparable protein structure, but different N-glycan composition. After mimicking the native ASP N-glycosylation via the expression in Nicotiana benthamiana plants, immunisation of calves with these plant-produced recombinants resulted in a significant reduction of 39% in parasite egg output, comparable to the protective efficacy of the native antigen. This study provides a valuable workflow for the development of recombinant vaccines against other parasitic nematodes.
Distributions of easy axes and reversal processes in patterned MRAM arrays
Authors: Frost William , Carpenter Robert , Couet Sebastien , O’Grady Kevin , Vallejo Fernandez Gonzalo 
Published: November 2023

AbstractThe distribution of the easy-axes in an array of MRAM cells is a vital parameter to understand the switching and characteristics of the devices. By measuring the coercivity as a function of applied-field angle, and remaining close to the perpendicular orientation, a classic Stoner-Wohlfarth approximation has been applied to the resulting variation to determine the standard deviation,$$\sigma $$σ, of a Gaussian distribution of the orientation of the easy-magnetisation directions. In this work we have compared MRAM arrays with nominal cells sizes of 20 nm and 60 nm and a range of free layer thicknesses. We have found that a smaller diameter cell will have a wider switching-field distribution with a standard deviation$$\sigma = {9.5}^{\circ }$$σ=9.5∘. The MRAM arrays consist of pillars produced by etching a multilayer thin film. This value of$$\sigma $$σis dominated by pillar uniformity and edge effects controlling the reversal, reinforcing the need for ever-improving etch processes. This is compared to larger pillars, with distributions as low as$$\sigma = {5.5}^{\circ }$$σ=5.5∘. Furthermore we found that the distribution broadens from$$\sigma = {5.5}^{\circ }$$σ=5.5∘to$$\sigma = {8.5}^{\circ }$$σ=8.5∘with free layer thickness in larger pillars and that thinner films had a more uniform easy-axis orientation. For the 20 nm pillars the non-uniform size distribution of the pillars, with a large and unknown error in the free-layer volume, was highlighted as it was found that the activation volume for the reversal of the free layer 930 nm$$^3$$3was larger than the nominal physical volume of the free layer. However for the 60 nm pillars, the activation volume was measured to be equal to one fifth of their physical volume. This implies that the smaller pillars effectively reverse as one entity while the larger pillars reverse via an incoherent mechanism of nucleation and propagation.
New Au/chitosan nanocomposite modified carbon paste sensor for voltammetric detection of nicotine
Authors: Shehata M. , Zaki M. , Fekry Amany M. 
Published: November 2023

AbstractA profoundly touchy voltammetric sensor for detection of nicotine (NIC) in urine and tobacco specimens has been developed in light of the boosted electrochemical response of NIC at gold and chitosan nanocomposite modified carbon paste electrode (ACMCPE). Material characterization techniques Scanning Electron Microscope and Energy Dispersive X-ray (SEM & EDX) were utilized to describe the ACMCPE surface material. The impedance spectroscopy technique (EIS), cyclic voltammetry (CV), chronoamperometry (CA), and differential pulse voltammetry (DPV) were employed to explore the electrochemical sensing of NIC at ACMCPE. The created sensor exhibits an exceptional electrochemical sensitivity to NIC in a universal Britton–Robinson (B-R) buffer solution with a pH range of 2.0 to 8.0. The sensor shows a linear response over NIC concentration ranges of 4.0–320.0 µM, with the detection limit (LOD) of 7.6 µM. The prepared sensor has been shown to be exceptionally viable in detecting NIC with amazing selectivity and reproducibility. We suggest it as a trustworthy and useful electrochemical sensor for NIC location.
Unveiling the potential role of natriuretic peptide receptor a isoforms in fine-tuning the cGMP production and tissue-specific function
Authors: Ang Wei Fong , Liao Dan , Koh Cho Yeow , Kini R. Manjunatha 
Published: November 2023

AbstractAtrial natriuretic peptide (ANP) is a peptide hormone that regulates blood pressure and volume. ANP interacts with natriuretic peptide receptor-A (NPR-A) to lower the blood pressure through vasodilation, diuresis and natriuresis. Previously, we designed two human ANP analogues, one with exclusively diuretic function (DGD-ANP) and the other with exclusively vasodilatory function (DRD-ANP). Although both ANP analogues interact with NPR-A, their ability to produce cGMP was different. Three alternatively spliced isoforms of NPR-A were previously identified in rodents. Here, we evaluated the putative human isoforms for their cGMP production independently and in combination with WT NPR-A in various percentages. All three NPR-A isoforms failed to produce cGMP in the presence of ANP, DGD-ANP, or DRD-ANP. Co-expression of isoforms with WT NPR-A were found to significantly impair cGMP production. Considering the differential tissue expression levels of all three spliced isoforms in rodents have previously been demonstrated, the existence of these non-functional receptor isoforms may act as negative regulator for ANP/NPR-A activation and fine-tune cGMP production by WT NPR-A to different degree in different tissues. Thus, NPR-A isoforms potentially contribute to tissue-specific functions of ANP.
Modeling fashion as an emergent collective behavior of bored individuals
Authors: Seiler Johannes P.-H. , Rumpel Simon 
Published: November 2023

AbstractBoredom is an aversive mental state that is typically evoked by monotony and drives individuals to seek novel information. Despite this effect on individual behavior, the consequences of boredom for collective behavior remain elusive. Here, we introduce an agent-based model of collective fashion behavior in which simplified agents interact randomly and repeatedly choose alternatives from a circular space of color variants. Agents are endowed with a memory of past experiences and a boredom parameter, promoting avoidance of monotony. Simulating collective color trends with this model captures aspects of real trends observed in fashion magazines. We manipulate the two parameters and observe that the boredom parameter is essential for perpetuating fashion dynamics in our model. Furthermore, highly bored agents lead future population trends, when acting coherently or being highly popular. Taken together, our study illustrates that highly bored individuals can guide collective dynamics of a population to continuously explore different variants of behavior.
Factors for achieving target serum uric acid levels after initiating urate-lowering therapy in patients with gout: results from the ULTRA registry
Authors: Do Hyunsue , Choi Hyo Jin , Choi Byoongyong , Son Chang-Nam , Kim Sang-Hyon , Choi Se Rim , Kim Ji Hyoun , Kim Min Jung , Shin Kichul , Kim Hyun-Ok , Song Ran , Lee Sung Won , Ahn Joong Kyong , Lee Seung-Geun , Lee Chang Hoon , Son Kyeong Min , Moon Ki Won 
Published: November 2023

AbstractAchieving target serum uric acid (SUA) levels is important in gout management. Guidelines recommend lowering SUA levels to < 6 mg/dL; however, many patients fail to reach this target, even with uric acid-lowering therapy (ULT). This study investigated clinical characteristics of target SUA achievers among Korean patients with gout. This study used data from the ULTRA registry, a nationwide inception cohort established in September 2021 that enrolls patients with gout who initiate ULT. Demographic, clinical, and laboratory data were collected at baseline; the 6-month follow-up. Patients were divided into two groups: target achievers (SUA level < 6 mg/dL at 6 months) and non-achievers. The mean participant (N = 117) age was 56.1 years, and 88.0% were male. At 6 months, 83 patients (70.9%) reached target SUA levels. Target achievers had better drug adherence (≥ 80%) to ULT (97.6% vs. 76.5%; p < 0.01) than non-achievers. Target non-achievers had a higher percentage of a family history of gout (32.4% vs. 10.8%; p < 0.01) and less antihypertensive agent use (38.2% vs. 59.0%; p = 0.03) than target achievers. Multivariate regression analysis revealed that good adherence to ULT, the absence of a family history of gout, and antihypertensive agent use were key factors associated with achieving target SUA levels at 6 months.
Chromosome genomics facilitates the marker development and selection of wheat-Aegilops biuncialis addition, substitution and translocation lines
Authors: Farkas András , Gaál Eszter , Ivanizs László , Blavet Nicolas , Said Mahmoud , Holušová Kateřina , Szőke-Pázsi Kitti , Spitkó Tamás , Mikó Péter , Türkösi Edina , Kruppa Klaudia , Kovács Péter , Darkó Éva , Szakács Éva , Bartoš Jan , Doležel Jaroslav , Molnár István 
Published: November 2023

AbstractThe annual goatgrass, Aegilops biuncialis is a rich source of genes with considerable agronomic value. This genetic potential can be exploited for wheat improvement through interspecific hybridization to increase stress resistance, grain quality and adaptability. However, the low throughput of cytogenetic selection hampers the development of alien introgressions. Using the sequence of flow-sorted chromosomes of diploid progenitors, the present study enabled the development of chromosome-specific markers. In total, 482 PCR markers were validated on wheat (Mv9kr1) and Ae. biuncialis (MvGB642) crossing partners, and 126 on wheat-Aegilops additions. Thirty-two markers specific for U- or M-chromosomes were used in combination with GISH and FISH for the screening of 44 Mv9kr1 × Ae. biuncialis BC3F3 genotypes. The predominance of chromosomes 4M and 5M, as well as the presence of chromosomal aberrations, may indicate that these chromosomes have a gametocidal effect. A new wheat-Ae. biuncialis disomic 4U addition, 4M(4D) and 5M(5D) substitutions, as well as several introgression lines were selected. Spike morphology and fertility indicated that the Aegilops 4M or 5M compensated well for the loss of 4D and 5D, respectively. The new cytogenetic stocks represent valuable genetic resources for the introgression of key genes alleles into wheat.
Topological electromagnetic waves in dispersive and lossy plasma crystals
Authors: Qian Chen , Jiang Yue , Jin Jicheng , Christensen Thomas , Soljačić Marin , Kildishev Alexander V. , Zhen Bo 
Published: November 2023

AbstractTopological photonic crystals, which offer topologically protected and back-scattering-immune transport channels, have recently gained significant attention for both scientific and practical reasons. Although most current studies focus on dielectric materials with weak dispersions, this study focuses on topological phases in dispersive materials and presents a numerical study of Chern insulators in gaseous-phase plasma cylinder cells. We develop a numerical framework to address the complex material dispersion arising from the plasma medium and external magnetic fields and identify Chern insulator phases that are experimentally achievable. Using this numerical tool, we also explain the flat bands commonly observed in periodic plasmonic structures, via local resonances, and how edge states change as the edge termination is periodically modified. This work opens up opportunities for exploring band topology in new materials with non-trivial dispersions and has potential radio frequency (RF) applications, ranging from plasma-based lighting to plasma propulsion engines.