bluuebunny/crossref_metadata_2025_split huggingface dataset:
#204 : First Recorded Normal Live Birth After ICSI with Electrophoretically Isolated Spermatozoa Using the Felix™ System
Authors: Jayaram Ramya , Govindarajan Mirudhubashini 
Published: January 2024

Background and Aims: This report describes the first live birth with sperm isolated using the Felix™ system. Methods: Thirty-three couples undergoing ovarian stimulation were recruited based on high raw semen DNA Fragmentation Index (DFI) of >20%, as assessed by the sperm chromatin structure assay. The Felix™ system, which isolates sperm based on charge and size was used as the sperm preparation method, over a six-minute interval. Mature oocytes were injected using Intracytoplasmic Sperm Injection (ICSI). Normally fertilised zygotes were cultured until day three or five. Suitable embryos were vitrified and subsequently transferred in a frozen embryo cycle. Biochemical pregnancy was determined by plasma human chorionic gonadotrophin level of >20 IU and clinical pregnancy was determined by the presence of fetal heat and/or sac on ultrasound. Miscarriage and live births rates were also recorded. Results: Raw semen concentration, progressive motility and total motility levels were in the normal range according to 6[Formula: see text] World Health Organisation criteria. However, sperm DFI was relatively high (range 20% to 80%). The average age of females was 30 years (range 21 to 44). A total of 374 oocytes were collected from the 33 females, with 266 mature oocytes then injected by ICSI. This resulted in 83% fertilisation rate and embryo utilisation of 65%. Four females underwent single embryo transfer, and 29 females underwent double embryo transfer. Of those, 19 (58%) biochemical pregnancies were achieved resulting in 14 (42%) ongoing clinical pregnancies. Three miscarriages and two normal live births have been reported to date. Interestingly, the two live births resulted from men of DFI of 80% and 33%. Conclusion: This report describes the first birth after sperm isolated using the Felix™ system followed by ICSI. The Felix™ system is an efficient procedure to prepare sperm and has the potential to be advantageous for patients with high DFI.
#116 : Prevalence of Aneuplodies in Chromosomes 13, 18, 21, X and Y in Embryos Generated by ICSI of Fertile Couples
Authors: Khan Shezae 
Published: January 2024

Background and Aims: It has previously been reported that up to 70% of the embryos generated in an ART lab are chromosomally abnormal. Abnormalities in chromosomes 13, 18, 21, X and Y have been observed to be as common as 63% in embryos of sub-fertile couples. The aim of this study was to find if these abnormalities are just as common in embryos of fertile couples who have no history of an affected child. Method: A retrospective cohort study was conducted on 409 embryos obtained from 113 couples undergoing preimplantation genetic testing at an ART center in Pakistan, between 1 January 2021 and 31 December 2022. The inclusion criteria was that fertile couples who had come for gender selection for family balancing. Age range of female partners was 28 years to 38 years. Male partners had normal semen parameters as per the World Health Organization (WHO) guidelines. Results: A total of 70.7% (289/409) were unaffected by aneuploides in the five chromosomes. There was a significant difference in the ratio of normal to abnormal embryos. Out of the normal embryos, there was no significant difference in the male (34.5%) and female (36.1%) embryos. There was no significant difference in the number of abnormal female and male embryos (p=0.127). Among the aneuploidies in the five chromosomes, trisomy 21 was the most common (n=27; 6.6%). Conclusion: Approximately 20% of sub-fertile couples have an unknown etiology. Our results indicate that the five commonly known aneuploidies found in embryos of sub-fertile patients have a low prevalence in fertile patients. It is worth noting that this study is limited by the fact that abnormalities in sub-fertile couple were not analyzed and statistics from other already published studies was relied upon. Nonetheless, this data has provided perspectives for counselling couples of unexplained sub-fertility.
#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
Authors: Li Chia-Jung , Lo Wei-Che , Sung Yen-Ju , Hsu Le-Tien , Chen Liang-Hsuan , Huang Shang-Yu , Chang Chia-Lin , Huang Hong-Yuan , Lee Chyi-Long , Sung Yung-Kuei , Wu Hsien-Ming 
Published: January 2024

Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy.
#152 : Exploring the Limitations of Linkage-Only PGT-M
Authors: Farrell Michael , Grkovic Steve , Traversa Maria , Jones Daniel , Livingstone Mark , McArthur Steven 
Published: January 2024

Background and Aims: Although PGT-M cases can be conducted using linkage only, the inclusion of a direct test targeting the relevant genetic variants should be fundamental to best practise. We present four cases illustrating the limitations of linkage only PGT-M. Methods: Three cases used STRs for linkage analysis and the fourth Illumina Infinium Karyomapping. Direct variant detection encompassed Sanger sequencing, STRs and/or fragment PCR. Results: Case 1 Osteoporosis-pseudoglioma syndrome Sanger sequencing for LRP5c.3107G[Formula: see text]A and LRP5c.4487C[Formula: see text]A were critical for identifying a recombination in the familial control and subsequent correct assignment of STR phasing. Case 2 Osteogenesis imperfecta type I SNP-based linkage identified a recombinant embryo. Sanger sequencing for COL1A1c.2461G[Formula: see text]A resolved an unknown status to affected. Case 3 Neurofibromatosis type II Variant detection in this de novo mosaic case used two STRs within the NF2 exon2-11del to establish low- and high-risk linkage patterns. Two tumour samples with different second hit mutations exhibited reduced signal intensity for one allele. This was consistent with the presence of a weaker deleted allele due to sample contamination with non-tumour cells. Four of nine embryos contained two alleles within the minimum deletion and were first choice for transfer, resulting in an NAD prenatal confirmation and healthy liveborn. Case 4 Neurofibromatosis type I A de novo mosaic case required reverse linkage by means of a direct assay for the NF1c.1445_1446insCC variant. Affected embryos were subsequently used to phase flanking STRs and obtain linkage, resulting in one liveborn and one ongoing pregnancy. Conclusions: When a direct test is not included in PGT-M, a family may not be able to be offered a test, or misdiagnosis risk may increase. For de novo, mosaic and cases with suspected recombination in a reference sample, analysis of multiple cycles and/or the inclusion of non-clinical embryos to increase sample numbers improves accuracy of testing.
#365 : Characteristics of Embryo Mosaicism Detected Using Next-Generation Sequencing PGT-A
Authors: Ikhsan Muhammad , Iffanolida Pritta Ameilia , Mutia Kresna , Muharam Raden , Wiweko Budi , Sumapraja Kanadi , Pratama Gita , Harzif Achmad Kemal , Maidarti Mila , Silvana Vita , Hestiantoro Andon 
Published: January 2024

Background and Aims: Chromosomal mosaicism may occur in minority of embryos. Their commonality and uncertainty made the embryo mosaic rates vary from center to center. This study aims to evaluate the mosaicism rate and its characteristics during our Preimplantation Genetic Testing-A (PGT-A) service in Indonesia since 2020. Method: We collected secondary data from our PGT-A results retrospectively since 2020. There were 196 embryos analyzed and descriptively shown for its characteristic including which chromosome is the most prevalent. We also carried out associative analysis to assess mosaicism and its clinical condition. Results: From 196 embryos assessed, there were 106 (54.1%) embryos with chromosomal abnormality ranging from low mosaicism to whole chromosome aneuploidy. Low and high mosaicism rates were found in 25 (12.8%), 8 (4.1%) embryos, respectively. The most common chromosome affected by low mosaicism is chromosome 8 (9; 4.6%) and the most chromosome with abnormality found in chromosome 21 (20; 10.2%). There was no significant difference between mosaicism rate in the mean age (33.88 vs 33.26 years), but there was statistical significance difference in mean age (35.84 vs 33.26 years) for aneuploidy (monosomy or trisomy) compared to normal chromosome. For the clinical condition, we found only significant difference in high mosaicism rate for patient with indication for unknown etiology of infertility (p-value [Formula: see text]0.05%). Conclusion: The mosaicism rate found in our center is relatively high compared to studies conducted elsewhere. The most common chromosome affected is chromosome 8. There was a significant difference in high mosaicism rate for unknown infertility indication of PGT-A.
#19 : Green Tea Extract Inhibits Endometriosis Progression by Increasing Superoxide Dismutase Serum Levels in the Rat Models
Authors: Trisetiyono Yuli , Artanto Yosef adi 
Published: January 2024

Background and Aims: Endometriosis is a gynecological disorder defined as the presence of endometrial tissue outside the uterine cavity and affecting 10-15% of females of reproductive age. Oxidative stress has been reported as one of the main promoting factors of this disease. Green tea and its anti-angiogenic and antioxidant properties have promising potential to prevent the progression of endometriosis. This study aims to investigate the effect of green tea extract on endometriotic implant area and serum superoxide dismutase (SOD) levels in endometriosis rat models. Method: Twenty-six Balb/c female rats were randomized into two groups of equal size: experimental group (EG) and control group (CG). All subjects in EG were given green tea extract 3 mg daily for 14 days. Endometriosis was induced on the fourteenth day in all subjects in both groups. The data of the endometriotic implant area and serum SOD were obtained and analyzed fourteen days after the induction of endometriosis. Results: The endometriotic implant area of the group treated with green tea extract was significantly lower (p=0,022) RR=0,455, 95%CI=0.065-0,96) compared to the control group. The serum level of SOD in EG and CG were 5.33±0.52 ng/ml and 5.20±0.91 ng/ml, respectively (p=0.507). Conclusion: Green tea extract inhibits endometriotic implant progression and increases the level of serum SOD in endometriosis-induced rat models.
#125 : The Role of BDNF Receptors in the Incidence of Endometrioma Tissue Invasion Onto the Chorioallantoic Membrane
Authors: Dewanto Agung , Rahmana Muhammad Dimas Reza , Arumsari Regina , Khasanah Nurida , Nugraha Wicesa , Trizia Vanessa , Anshori Khoiruddin 
Published: January 2024

Background and Aims: Previous studies have explored the role of neurotrophins and their receptors, especially in forming deep endometriosis, endometriosis tissue invasion, and its effects on tissue proliferation. However, these studies do not include research on the pathogenesis of endometriomas. We tried to model the invasion of endometrioma tissue to find out more about its pathogenesis by using the chorioallantoic membrane (CAM) as the host. Immunohistochemistry (IHC) to detect neurotrophin BDNF and its receptors, namely TrkB and P75, are employed and linked between their expression and the process of invasion and proliferation. Method: The endometriotic tissue samples were collected from women (n=27) who underwent hysteroscopy/laparoscopy at Dr. Sardjito Central Hospital. Peritoneal endometriosis (PE) lession, endometrioma (CC), and eutopic endometrium (EN) was analyzed with 15 tissue samples in each group. Samples were placed in a tube containing transport medium and transplanted into CAM of fertile chicken eggs for five days. Transplanted tissue was harvested, and histological preparations were made using the paraffin method. IHC staining was performed on p75, Ki67, TrkB, and BDNF staining. Invasion analysis and IHC evaluation were performed with a semi-quantitative method. Data were analyzed using IBMⓇ SPSS Statistics version 25.0. Statistical significance was accepted at P < 0.05. Results: Normal Endometrium expressed the highest expression of BDNF than peritoneal endometriosis and endometrioma (0.36± 0.38, 0.16± 0.13, 0.07± 0.07, P=0.007). The P75 expression correlated positively with Ki67 expression in PE and CC samples (P=0.012; P=0.008). Conclusion: The p75 receptor may have a role in endometriosis tissue proliferation, but this receptor does not directly influence tissue invasion into CAM. While proliferation in endometriomas positively correlates with invasion into CAM, it is not correlated with peritoneal endometriosis.
#151 : Partial Urorectal Septum Malformation Sequence
Authors: Braña Jennifer Clarize , Alcantara-Boquiren Marie Janice , Pacioles - Flavier Carol Marjorie 
Published: January 2024

Background and Aims: Cloacal malformations represent a wide range of defects and are considered one of the most challenging and interesting diseases. Occurrence is at 1 in 50,000 newborns and still with an unknown etiology. Partial urorectal septal malformation sequence (URSMS) is a rare and complex congenital anomaly that represents an embryonic event of urinary-rectal septal malformation resulting in the presence of a cloaca. A 17-year-old nulligravid with imperforate anus at birth with primary amenorrhea was admitted for difficulty urinating. Genital examination noted fused labia with vaginal dimple and absent urethral opening. Currently, there are no field studies of its incidence in the Philippines and no exact treatment has been described. This case aims to assist gynaecologists in diagnosing and treating patients with this malformation and to help future patients achieve a good quality of life and to be able to function sexually later in life despite these set of conditions. Method: Abdominal CT scan, 3D ultrasound and MRI all showed uterine didelphys with vaginal duplication and bilateral hydrometrocolpos and vaginal aplasia. A diagnosis of partial URSMS was made using a complete medical history and the above diagnostic procedures correlating with PE. A multidisciplinary conference was held with the agreed plan of laparoscopy, hysterectomy, vaginal septum repair, cystoscopy, urethroplasty, and feminizing genitoplasty. Results: The opening of the neovagina admitted two fingers and was 4 to 5 centimeters long. Three months after reconstruction, she reported having normal urination with amenorrhea and no recurrence of pelvic pain. Conclusion: Early and accurate preoperative diagnosis and treatment planning, careful patient selection, and a holistic approach are cornerstones of management. Patient follow-up and long-term psychosocial support are important, as obstruction may recur in poorly compliant patients. If pregnancy does occur in these patients, a multidisciplinary approach to management is important, and the recommendation for delivery is usually cesarean delivery.
#393 : Obstructed Hemivagina and Ipsilateral Renal Anomaly (OHVIRA) — A Series of 6 Cases at a Tertiary Referral Centre
Authors: Lee Nikki , Ng Karen , Ip Patricia , Chan Symphorosa 
Published: January 2024

Background and Aims: OHVIRA is a rare congenital urogenital anomaly. Diagnosis is often delayed because patients have normal onset of puberty and menstruation if there is partial obstruction with a communicating fenestration in the hemivagina. Symptoms can be non-specific such as abdominal pain and dysmenorrhoea, but urinary retention and a pelvic mass have been reported. Haematocolpos can cause anatomical distortion, making imaging difficult. Definitive management is by vaginoplasty but that can be complex, especially in an anatomically small child, or cases with chronic inflammation. Retrograde bleeding during obstruction is common, leading to endometriosis and ascending infection. This is associated with complex surgery, increased morbidity and subfertility, which is why early diagnosis and timely management are important. Method: Medical records were retrieved from 6 patients with OHVIRA treated between 1 April 2021 and 31 March 2023 at a tertiary referral centre. For each patient, age of diagnosis, referral source, symptoms, diagnostic investigations, diagnostic challenges, anatomical findings, surgical managements, complications and outcomes were reviewed. Results: Patient’s ages ranged from 10 to 33 years. Median age of diagnosis was 30. Cases were referred from paediatric surgery (n=1), gynaecology (n=2), general surgery (n=2) and urology (n=1). Symptoms included abdominal pain (n=4), pyrexia (n=2), intra-menstrual bleeding (n=2) and vaginal discharge (n=1). 3 patients had been misdiagnosed before referral. All had unilateral haematocolpos and underwent ultrasound (n=6) and either MRI (n=4) or CT (n=2), followed by vaginoplasty. 2 patients had emergency surgery for pelvic abscesses. There were no intra- or post-operative complications, or adverse outcomes. Conclusion: Median age at diagnosis was advanced, and symptoms and referral source were variable. Misdiagnosis was frequent. Surgical management was successful with a single stage vaginoplasty. Ascending infection can be a consequence of delayed diagnosis and management. It is important to evaluate any female with dysplastic or absent kidneys for uterovaginal abnormalities.
#158 : Whole Transcriptome Analysis of In Vitro-Aged Mouse Oocytes: Effects of Antioxidant Mixture on Oocyte Quality
Authors: Qin Junwen , Takahashi Yuji , Kakuta A.P Shigeru , Kyuwa Shigeru 
Published: January 2024

Background and Aims: Aging is known to have negative impacts on oocyte quality. We reported in the previous meeting that the quality of in vitro-aged oocytes was restored by application of a new antioxidant mixture (AOM). Aiming to understand how AOM influenced on oocyte quality, a whole transcriptome analysis was conducted in this study. Method: Using an in vitro aging model, oocytes were collected from superovulated ICR mice, and part of them were cultured for 5h with (AOM group) or without (Aging group) AOM. Oocytes from fresh oocytes (Control group), Aging group and AOM group were subjected to RNA extraction, amplification, and a genechip microarray analysis. Genes expressed with absolute log2 fold change of [Formula: see text] 2 between two groups were designated as differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was conducted for further analysis of DEGs. Results: In vitro aging triggered a total of 643 DEGs when compared with the control group. These DEGs were enriched in 19 biological processes, mostly associated with ribosomal synthesis, metabolic process, response to oxidative stress, apoptosis, signal transduction and mRNA processing. These changes are thought to be related to decreasing oocyte quality. AOM effectively prevented age-related change of those DEGs in a broad spectrum. The genes restored by AOM including Rpl7, Rpl35a, Rpl8, Mt1, Fabp2, Acadm, Hadhb, Gstm4, Ncf4, Ppp3ca, Fzd9, Phka2, Spp1 and Lsm6, are highly associated with cellular homeostatic events, suggesting that AOM not only induces changes of response to oxidative stress, but also normalizes gene expression related to cellular activities resulting in improving oocyte quality. Conclusion: This study strongly suggests that AOM restores the gene expression involved in cellular homeostasis. Also, these findings provide new insights of oocyte quality.
#256 : Reproductive Outcomes and Satisfaction Among Patients Using Telemedicine During Assisted Reproduction: A Meta-Analysis
Authors: Tran Huy-Phuong , Nguyen Nam-Nhat , Ho Tuong-Nguyen , Tran Thuy-Thi-Thanh , Ly Loc-Thai , Hoang Tuyet-Thi-Diem , Le Dung-Thi-Phuong , Vo Toi-Van , Tran Giang-Le 
Published: January 2024

Background and Aims: Telemedicine refers to the delivery of healthcare services through the use of information and communication technology, across distances. There has been a significant increase in the demand for infertility treatment and virtual consultations from home after the COVID-19 pandemic. Despite this, due to a lack of sufficient data, and concerns about patient compliance, telemedicine has struggled to gain widespread acceptance. Our aim was to compare the success of treatment between telemedicine and in-office care during assisted reproduction treatment. Additionally, we evaluated patient satisfaction with telemedicine. Method: We conducted a literature search of published articles in the PubMed/MEDLINE, EMBASE, Scopus, and Web of Science databases up to November 30, 2022. We used the keywords “telemedicine” or “telehealth” and “in vitro fertilization” or “assisted reproduction”. Both randomized controlled trials and observational studies were included in the search. We registered our protocol on PROSPERO (Registration number: CRD42022379428). Results: We identified 14 studies that reported data on 5182 patients. Quality assessment revealed an acceptable risk of bias for both randomized controlled trials and observational studies. The data showed that telemedicine was non-inferior to in-person care in terms of pregnancy rate achieved (OR 1.02, 95% CI: 0.83–1.25, p = 0.84). The Q test p-value of 0.99 and I2 statistic of 0% indicated that all the included studies were homogeneous. Patients who received telemedicine follow-ups during fertility treatment reported adequate satisfaction (88%, 95% CI: 77%-94%). Eggers’ test did not indicate the presence of Deek’s funnel plot asymmetry (p = 0.62), confirming that no publication bias was found. Conclusion: Telemedicine use has resulted in a comparable treatment success rate in terms of pregnancy rate, when compared to face-to-face consultation. These emerging tools also yielded a high level of patient satisfaction.