bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transforming Food Industrial Sludge Into Sustainable Resources: Innovations in Waste Management and Renewable Energy Recovery
Authors: Aryanfar Yashar , García Alcaraz Jorge Luis , Keçebaş Ali , Fernandez Julio Blanco , Arslan Busra , Ilbas Mustafa , Algarni Salem , Alqahtani Talal , Irshad Kashif 
Published: February 2025

ABSTRACTThis study comprehensively investigates the potential of food industrial sludge as a renewable resource within the expanding global food industry. Grounded in the theoretical framework of the circular economy and sustainability sciences, it delves into the composition of sludge, comprising diverse organic (proteins, carbohydrates, lipids, and fibers) and inorganic elements (minerals, heavy metals, and trace elements), highlighting its environmental and economic implications. The focus is on evaluating key utilization methods—anaerobic digestion, thermal treatment (pyrolysis and gasification), composting, and bioconversion—for transforming sludge into valuable resources such as biogas, biochar, and compost. These methods are assessed based on their alignment with sustainable waste management theories and practices, particularly concerning environmental sustainability and resource recovery. Empirical data from case studies and industry reports are incorporated to provide concrete examples of successful sludge utilization practices. For instance, empirical data indicate that anaerobic digestion can reduce sludge volume by up to 70% and generate biogas with an energy yield of approximately 25 MJ per kg of dry sludge. Pyrolysis can sequester up to 3 t of CO2 per ton of biochar produced. These methods demonstrate considerable promise for energy production, nutrient recovery, and reducing greenhouse gas emissions, supporting a circular economy approach. The study addresses challenges in sludge management, including compositional variability, contaminant presence, and the necessity for effective treatment to mitigate environmental risks such as water and soil pollution and odor issues. Findings indicate that the sustainable utilization of food industrial sludge as a renewable resource is not only viable but also essential for minimizing environmental impact, conserving finite resources, and promoting sustainability in the food industry. This research contributes valuable insights for policymakers, industry stakeholders, and environmental scientists, enriching the body of knowledge on waste management and resource recovery. The research is framed within the context of Sustainable Development Goals (SDGs), particularly SDG 6 (Clean Water and Sanitation), SDG 7 (Affordable and Clean Energy), and SDG 12 (Responsible Consumption and Production).
Haploidentical Stem Cell Transplantation With Dual Source of Cells and Post‐Transplant Cyclophosphamide
Authors: Fonseca‐Hial Ana Marcela Rojas , Parisio Katya , de Oliveira Jose Salvador Rodrigues 
Published: February 2025

ABSTRACTBackgroundDual sources of cells (DSC) with peripheral blood stem cell apheresis (PBSC) and surgical bone marrow (BM) for haploidentical hematopoietic cell transplantation (Hid‐HCT) are used in China and some Asian countries. The experience of the Baltimore group for haploidentical transplant with post‐transplant cyclophosphamide (PT‐Cy) and reduced‐intensity‐conditioning (RIC) regimen used BM as a source of hematopoietic stem cells.MethodsWe retrospectively analyzed 64 Hid‐HCT with DSC and PT‐Cy, RIC (n = 57), or myeloablative‐conditioning (MAC) (n = 7), from two public health Brazilian centers, with a median follow‐up of 23.3 months (6.7–45.4).ResultsThe 49 malignant patients were 27/46 (58.7%) beyond the first remission or with no complete response, and three patients did not complete disease status evaluation before transplant. Eight of 62 patients (12.9%) had grade 2 or more Hematopoietic Cell Transplantation‐Specific Comorbidity Index (HCT‐CI), and two patients had no HCT‐CI classified. Cytomegalovirus (CMV) viremia occurred in 26 of 57 (45.6%). The cumulative incidence of 100‐day grade III‐IV acute GVHD was 12.3% (7/57), with a 95% confidence interval (CI) of 3.8% and 20.8%, and 2‐year moderate or severe chronic GVHD was 21.1% (11/52; 95% CI, 10.1%–32.3%). The 2‐year relapses were 24.5% for malignant disease (12/49; 95% CI, 12.4%–36.5%). The 2‐year overall survival (OS) probability was 54.7% (35/64; 95% CI, 42.5%–66.9%). Benign diseases achieve 2‐year OS in 73.3% (11/15; 95% CI, 51%–95.7%) of the patients. The HCT‐CI were significant in multivariate analyses for DFS (p = 0.002) and OS in uni‐ and multivariate analyses (both p < 0.001). The number of CD34+ cells by apheresis collection was significant in multivariate analysis for DFS (p = 0.039).ConclusionHid‐HCT using PT‐Cy, DSC, and RIC is a safe option for benign and malignant diseases.
Dual Relaxant Effect of Coumarin‐3‐carboxamides Through the Nitric Oxide/Cyclic Guanosine Monophosphate Pathway and Ca2+ Channels Blockade
Authors: Jiménez‐Rodríguez Arabelly , Alvarado‐Sánchez Cuauhtémoc , Velasco Manuel , López‐Oliva Estephania , Romero‐Ceronio Nancy , Ortiz‐Andrade Rolffy , Hernández‐Abreu Oswaldo 
Published: February 2025

ABSTRACTWe aimed to determine the relaxant pathway of seventeen synthetic compounds derived from coumarin‐3‐carboxamide. An isolated rat aorta assay was used. To determine the vasorelaxant mode of action, receptor blockers and specific enzyme inhibitors involved in endothelial and smooth muscle signaling pathways were used. The compounds 2, 4, and 5 showed higher activity than the other compounds. N‐nitro‐L‐arginine methyl ester (10 µM) and methylene blue (10 µM) significantly inhibited the relaxant effect of the compounds 2, 4, and 5 (p ≤ 0.05), but not tetraethylammonium (5 mM), indomethacin (10 µM), or atropine (1 µM). The compounds 2, 4, and 5 abated the contraction induced by CaCl2. The compounds 2, 4, and 5 exert a relaxing effect through the nitric oxide/cyclic guanosine monophosphate pathway activation and by blocking Ca2+ channels of the cell membrane. These findings propose coumarin‐3‐carboxamides as new drug entities with the potential to develop non‐clinical and clinical.
Suppression of Fibroblast Growth Factor 23 in UMR106 Osteoblast‐Like Cells and MC3T3‐E1 Cells by Adipokine Chemerin
Authors: Vogt Julia , Daferner Kim , Föller Michael 
Published: January 2025

ABSTRACTEndocrine fibroblast growth factor 23 (FGF23) derived from bone governs phosphate and vitamin D metabolism. Paracrine FGF23 has additional functions in different organs. Moreover, plasma FGF23 is correlated with outcomes in chronic kidney disease. FGF23 regulation is complex depending on a plethora of different factors and conditions including AMP‐dependent kinase (AMPK), inflammation, and adipokines leptin and adiponectin. Chemerin is an adipokine implicated in proinflammatory processes in adipose tissue and other organs and an activator of AMPK. Here, we investigated whether chemerin is a regulator of FGF23. UMR106 osteoblast‐like cells and MC3T3‐E1 osteoblasts were studied. Gene expression was assessed by qRT‐PCR, FGF23 protein by ELISA, and AMPK activity by western blotting. Both cell lines expressed Cmklr1 encoding chemerin chemokine‐like receptor 1. Chemerin slightly but significantly reduced Fgf23 expression. Chemerin reduced FGF23 protein abundance in the cell culture supernatant, and RNAi‐mediated Cmklr1 silencing upregulated Fgf23 expression in UMR106 cells. In the presence of AMPK inhibitor compound C, chemerin failed to suppress Fgf23 in UMR106 cells. In conclusion, chemerin‐dependent Cmklr1 signaling downregulates FGF23 in bone cell lines. This effect requires, at least partly, AMPK.
Melatonin Prevents Thymic Atrophy but Does Not Protect Against Disruption of T Cell Maturation Related to Cyclophosphamide Exposure
Authors: Pimenta Gustavo F. , Dourado Thales M. H. , de Souza Kayse D. B. , Elias‐Oliveira Jefferson , Rodrigues Vanessa F. , Carlos Daniela , Tirapelli Carlos R. 
Published: January 2025

ABSTRACTIncreased oxidative stress and apoptosis are key mechanisms of thymic atrophy induced by cyclophosphamide (CYP). Atrophy leads to changes in the thymic microenvironment and disrupts T cell maturation. The hormone melatonin displays antioxidant and antiapoptotic effects. Here, we tested the hypothesis that melatonin would act as a cytoprotective agent against the harmful effects of CYP in the thymus. A single dose of CYP (300 mg/kg; ip) was injected in male C57BL/6 mice pretreated or not with melatonin (10 mg/kg/day, ip) for 4 days. Atrophy, oxidative stress and apoptosis markers, and T cell subpopulations were evaluated in the thymus 24 h after CYP injection. Melatonin partially prevented atrophy and the increase in caspase 3 activity induced by CYP. Augmented lipoperoxidation and generation of NADPH‐oxidase derived superoxide (O2•−), as well as decreased superoxide dismutase (SOD) activity, were detected in the thymus of CYP‐injected mice. Pretreatment with melatonin abrogated these responses. CYP reduced the number of double‐positive (CD4+CD8+) cells, activated single‐positive (CD8+ and CD4+) cells, and regulatory CD4+FoxP3+ (Treg) cells in the thymus. None of these effects were reversed by melatonin. In conclusion, melatonin partially prevented thymic atrophy, possibly by reducing apoptosis and oxidative stress. However, melatonin did not abrogate the immunomodulatory effect of CYP on T cell populations. The lack of effect of melatonin on CYP‐induced reduction in Treg cells may be of interest since these cells reduce antitumor immunity.
PRMT5 Inhibition Enhances Therapeutic Efficacy of Cisplatin via Mediating miR‐29b‐3p‐Mcl‐1 Expression in Lung Adenocarcinoma
Authors: Li Haichao , Fan Jiangjiang , Shen Weiwei , Zhang Yong , Zhu Ximing , Li Pei , Gu Zhongping , Jing Pengyu 
Published: February 2025

ABSTRACTCisplatin is one of the front‐line therapeutic agents used to treat cancers, while drug resistance is a great obstacle to anti‐tumor efficiency. Protein arginine methyltransferase 5 (PRMT5) has been identified as a promoter of tumorigenesis, motility, and invasion. Inhibiting PRMT5 reduced hypoxia‐induced carboplatin resistance in lung adenocarcinoma (LUAD). However, the specific relationship between PRMT5 and cisplatin (CDDP) warrants further investigation. Our research revealed that PRMT5 inhibitor C9 enhanced CDDP chemosensitivity by suppressing proliferation and promoting apoptosis in LUAD cells. Through examining pro‐apoptotic proteins regulated by PRMT5, we identified that Mcl‐1 played a significant role in PRMT5‐mediated CDDP chemosensitivity. Furthermore, PRMT5 regulated Mcl‐1 expression through mediating miR‐29b‐3p. In vivo, our research presented that C9 increased CDDP chemosensitivity in LUAD xenografts. All in all, our data raised an interesting possibility that epigenetic reprogramming was associated with chemosensitivity. PRMT5 inhibitor C9 improved CDDP effectiveness in LUAD cells by inhibiting Mcl‐1 expression via miR‐29b‐3p, thereby modulating cellular proliferation and apoptosis.
Discovery of Highly Active Noble‐Metal‐Lean Mo−Ru Electrocatalysts for Hydrogen Evolution
Authors: Cechanaviciute Ieva A. , Antony Rajini P. , Banko Lars , Quast Thomas , Pokharel Saika , Ludwig Alfred , Krysiak Olga A. , Schuhmann Wolfgang 
Published: January 2025

AbstractThe discovery of high‐performance electrocatalysts for water electrolysis is highly important. We use a strategy for catalyst discovery based on high‐throughput screening of a broad range of materials compositions on a thin‐film materials library to identify noble‐metal‐lean multi‐metal compositions with high activity towards the hydrogen evolution reaction in alkaline electrolyte. We demonstrate this strategy using a quaternary materials library containing Mo, Ag, Ti, and Ru fabricated by combinatorial magnetron sputtering on a 10 cm diameter wafer providing 342 measurement areas. Surprisingly, binary Mo−Ru‐containing catalyst compositions with comparatively low Ru content exhibited the highest activity. Using the polymer/metal precursor‐based spray technique, Ni foam electrodes were modified with the Mo−Ru hit compositions and evaluated in a model electrolyzer in membrane electrode assembly (MEA) configuration. The electrodes showed a very low overpotential of only 132 mV at a comparatively high current density of −2 A cm−2 and a 24 h electrolysis stability at −1 A cm−2 with no observable degradation after the initial electrode conditioning.
Direct Recycling of Cathode Materials from Spent Lithium‐Ion Batteries: Principles, Strategies, and Perspectives
Authors: Li Sihan , Wu Zhan , Zhang Miaoquan , Xu Jianping , Jin Zheyu , Gan Yongping , Xu Zhihong , Wang Qingli , Zhang Wenkui , Xia Yang , He Xinping , Zhang Jun 
Published: February 2025

AbstractIn recent years, lithium‐ion batteries have become an important part of the global transition to green and low‐carbon energy. However, due to the rapidly increasing demand and production of lithium‐ion batteries, there is a large amount of spent batteries that need to be disposed of. The most critical and valuable recycling of spent batteries is the recycling of cathode materials. Pyrometallurgy and hydrometallurgy are traditional recycling processes aimed at extracting valuable metal elements from cathode materials. However, these methods have several disadvantages, including destruction of the structure of cathode materials, lengthy repair processes, high energy consumption and high environmental pollution. The direct recycling process is a popular repair technology for cathode materials in lithium‐ion batteries. The aim is to restore or upgrade the cathode materials in a non‐destructive manner or convert them into other functional products for secondary use, characterized by a short repair process, high atom utilization, lower costs and lower carbon emissions. This perspective summarizes the current status of lithium‐ion battery recycling, with a focus on direct recycling of cathode materials. It describes the pretreatment process, theoretical foundations, direct regeneration strategies and perspectives and provides insights for relevant researchers.
Self‐Assembly of Quinazolinedione Rosettes
Authors: Yamada Yuhei , Hanayama Hiroki , Kajitani Takashi , Datta Sougata , Yagai Shiki 
Published: February 2025

AbstractA quinazoline‐2,4(1H,3H)‐dione bearing a phenylene moiety and aliphatic tails was synthesized as an alternative self‐assembling building block to barbiturate molecules, aiming to achieve enhanced hydrolysis resistance. The compound self‐assembles in non‐polar solvents to form linear supramolecular polymers via the formation of hydrogen‐bonded cyclic hexamers (rosettes), a process confirmed by powder X‐ray diffraction (PXRD) analysis of the bulk material. Our results demonstrate that quinazoline‐2,4(1H,3H)‐dione serves as an effective hydrogen‐bonding building block, suggesting its potential to form stable supramolecular polymers from versatile π‐conjugated molecules.
Divergent Synthesis of Four Types of Fused N‐Containing Heterocycles via Oxidative Multi‐Component Cyclization Reaction
Authors: Guo Xiaoshuang , Miao Maozhong , Zhao Peng , Ma Yongmin 
Published: February 2025

Comprehensive SummaryAn atmospheric‐oxygen‐mediated four‐component reaction was developed for the divergent synthesis of pyrazolo[1,5‐a]pyrimidine and pyrazolo[3,4‐b]pyridine derivatives from readily available alcohols and 3‐aminopyrazoles. In this transformation, atmospheric oxygen serves as the green oxidant, promoting alcohols as equivalent aldehydes for the synthesis of four types of N‐containing heterocycles (> 40 examples). Remarkably, the transformation features metal‐free and molecular diversity.
In Situ formed Organic Sodium Salt/rGO Nanocomposite as Anode Material for Sodium Ion Batteries
Authors: Xue Qianwen , Luo Yuansheng , Tu Xiaoxue , Yin Haoyu , Chen Jingfu , Wu Fei , Zhong Cheng , Zhu Linna 
Published: February 2025

AbstractSodium‐ion batteries (SIBs) are expected to be the next‐generation large‐scale energy storage technology. Organic anode materials are potential for efficient SIBs because they are not sensitive to the size of metal ions. Yet they still suffer from shortcomings such as low electrical conductivity, and solubility in electrolyte. Formation of nanocomposite with carbon materials is an efficient way to address these issues. Herein, we design a thiophene‐based carboxylate compound STT, and the STT@rGO nanocomposite is also in situ formed as anode material for SIBs. The reduced graphene oxide (rGO) could improve conductivity and decrease the solubility of the anode material. Compared to the pristine STT electrode, STT@rGO delivers a reversible specific capacity of 178 mAh g−1, with a capacity retention rate of 86 % after 1000 cycles. Moreover, full batteries are successfully assembled using the nanocomposite anode and the commercial cathode Na3V2(PO4)3, manifesting the potential applications of the nanocomposite as organic electrode materials.
Egg Retrieval as a Cognitive Indicator in Cuckoo Hosts
Authors: Zhong Guo , Wan Guixia , Wang Longwu , Liang Wei 
Published: January 2025

ABSTRACTEgg retrieval behavior in hosts within avian brood parasitism systems was found to be regulated by the motivation to reject parasitic eggs. However, due to the limitations in the research systems, there is a lack of effective validation regarding the adaptation mechanisms of cuckoo hosts to the conflict between retrieving their own eggs outside the nest and rejecting parasitic eggs. This study uses Daurian redstarts (Phoenicurus auroreus), a secondary cavity‐nesting host parasitized by common cuckoos (Cuculus canorus), to verify the adaptive decision‐making of the host between egg retrieval and egg rejection by simulating the occurrence of eggs outside the nest. The results showed that Daurian redstarts ignored 60.6% of highly mimetic conspecific eggs, with a retrieval rate of only 18.2%. Additionally, Daurian redstarts rejected 21.2% of conspecific eggs. However, non‐mimetic budgerigar (Melopsittacus undulatus) and white model eggs were more likely to be directly rejected (75% and 86.4%, respectively) with no retrieval events. Our findings suggest that egg retrieval behavior in Daurian redstarts is likely influenced by the cognitive process of rejecting parasitic eggs, leading to occasional over‐identification and difficulty in decision‐making between egg retrieval and egg rejection, especially in the context of conflicting motivations.
Unlocking the Link Between Gut Microbiota and Psychopathological Insights in Anorexia Nervosa: A Systematic Review
Authors: Scala Mauro , Tabone Mariangela , Paolini Marco , Salueña Andrea , Iturra Rocío Arroyo , Ferreiro Veronica Romero , Alvarez‐Mon Miguel Ángel , Serretti Alessandro , Soltero Maria del Rocío Gonzalez , Rodriguez‐Jimenez Roberto 
Published: January 2025

ABSTRACTObjectiveThis systematic review explores the associations between qualitative/quantitative changes in gut microbiota and psychopathological symptoms or other clinical features in patients with eating disorders (EDs). Secondary outcomes include exploring gut microbiota changes in EDs and potential relationships with psychotropic drug use.MethodA systematic search was conducted across biomedical databases from inception to June 2024 according to PRISMA guidelines. The risk of bias was assessed, and a narrative synthesis was performed due to the heterogeneity of the outcomes.ResultsOnly findings related to anorexia nervosa (AN) were identified. Ten studies, of which seven were longitudinal, two cross‐sectional, and one interventional (N = 350 patients with AN, and 304 HCs), were included. Despite no clear links between diversity metrics and clinical symptoms being observed, specific taxa belonging to phylum Firmicutes, such as Clostridium, Roseburia, Lactobacillus, Faecalibacterium, and Bifidobacterium belonging to Actinobacteriota correlated with ED psychopathology, including anxiety and depressive symptoms.ConclusionsChanges in microbiota were related to anxiety and depressive symptoms, as well as altered eating behaviours by modulating inflammation and insulin pathways through short‐chain fatty acids (SCFAs), that also lead to neurotransmitter imbalances. Further studies are required to replicate these finding and to explore whether similar patterns are observed in other EDs.
A Statistical Characterization of Dynamic Brain Functional Connectivity
Authors: Chow Winn W. , Seghouane Abd‐Krim , Seghier Mohamed L. 
Published: February 2025

ABSTRACTThis study examined the statistical underpinnings of dynamic functional connectivity in mental disorders, using resting‐state fMRI signals. Notably, there has been an absence of research demonstrating the non‐stationarity of the empirical probability distribution of functional connectivity. This gap has prompted debate on the existence of dynamic functional connectivity, leading skeptics to question its relevance and the reliability of research findings. Our aim was to fill this gap by conducting a comprehensive empirical distribution analysis of functional connectivity, using Pearson's correlation as a measure. We conducted our analysis on a set of preprocessed resting‐state fMRI samples obtained from 186 subjects selected from the UCLA Consortium for Neuropsychiatric Phenomics dataset. Departing from conventional methods that aggregated signals over voxels within a region of interest, our approach leveraged individual voxel signals. Specifically, our approach offered a precise characterization of the empirical probability distribution of resting‐state fMRI signals by evaluating the temporal variations and non‐stationarity in dynamic functional connectivity, as measured by Pearson's correlation. Our study investigated functional connectivity patterns across 49 regions of interest, comparing healthy control subjects with patients diagnosed with ADHD, bipolar disorder, and schizophrenia. Our analysis revealed that (1) the empirical distribution of the correlation coefficient exhibited non‐stationarity, (2) the beta distribution was an accurate approximation of the exact correlation coefficient distribution, and (3) the empirical distribution of means derived from the fitted beta distributions, unraveled distinctive dynamic functional connectivity patterns with potential as biomarkers associated with different mental disorders. A key contribution of our study was the presentation of the first comprehensive empirical distribution analysis of dynamic functional connectivity, thus providing compelling evidence for its existence. Overall, our study presented an innovative statistical approach that advances our understanding of the dynamic nature of functional connectivity patterns derived from resting‐state fMRI. Our examination of the empirical distribution of dynamic functional connectivity provided solid evidence supporting its existence. The distinctive dynamic functional connectivity patterns we identified across various mental disorders hold promise as potential biomarkers for further development.
The Effects of Resigning GPs on Patient Healthcare Utilization and Some Implications for Health
Authors: Monsees Daniel , Westphal Matthias 
Published: January 2025

ABSTRACTWe study the effects of general practitioners' (GPs') resignations on their patients' healthcare utilization and diagnoses in an event‐study setting. Using claims data from a large German statutory health insurance, we find that after physicians leave, their former patients persistently reduce their primary care utilization, only partially substituting it with specialist visits and hospital care. Because patients find a new GP already 1.1 quarters after the old resigns, on average, the persistent effects must be explained through the new GP. Indeed, the new GP serves more patients but performs less diagnostic testing. Our results reveal a substantial decrease in diagnoses of many relevant chronic conditions (such as congestive heart failure and diabetes), suggesting that disruptions may have adverse consequences for the efficiency of the healthcare system. This indicates that continuity in primary care is pivotal and shows that the GP has an essential role in healthcare delivery, particularly in healthcare systems such as Germany, where GPs often have a high workload and little consultation time.