bluuebunny/crossref_metadata_2025_split huggingface dataset:
PER2 regulates odontoblastic differentiation of dental papilla cells in vitro via intracellular ATP content and reactive oxygen species levels
Authors: Ma Haozhen , Sheng Xinyue , Chen Wanting , He Hongwen , Liu Jiawei , He Yifan , Huang Fang 
Published: December 2023

Background
Dental papilla cells (DPCs) are one of the key stem cells for tooth development, eventually forming dentin and pulp. Previous studies have reported that PER2 is expressed in a 24-hour oscillatory pattern in DPCs in vitro. In vivo, PER2 is highly expressed in odontoblasts (which are differentiated from DPCs). However, whether PER2 modulates the odontogenic differentiation of DPCs is uncertain. This research was to identify the function of PER2 in the odontogenic differentiation of DPCs and preliminarily explore its mechanisms.

Methods
We monitored the expression of PER2 in DPCs differentiated in vivo. We used PER2 overexpression and knockdown studies to assess the role of PER2 in DPC differentiation and performed intracellular ATP content and reactive oxygen species (ROS) assays to further investigate the mechanism.

Results
PER2 expression was considerably elevated throughout the odontoblastic differentiation of DPCs in vivo. Overexpressing Per2 boosted levels of odontogenic differentiation markers, such as dentin sialophosphoprotein (Dspp), dentin matrix protein 1 (Dmp1), and alkaline phosphatase (Alp), and enhanced mineralized nodule formation in DPCs. Conversely, the downregulation of Per2 inhibited the differentiation of DPCs. Additionally, downregulating Per2 further affected intracellular ATP content and ROS levels during DPC differentiation.

Conclusion
Overall, we demonstrated that PER2 positively regulates the odontogenic differentiation of DPCs, and the mechanism may be related to mitochondrial function as shown by intracellular ATP content and ROS levels.
Nature-Based Interventions for Autistic Children
Authors: Fan Myrian Sze Nga , Li William Ho Cheung , Ho Laurie Long Kwan , Phiri Lophina , Choi Kai Chow 
Published: December 2023

ImportanceThere is a gap in the evidence regarding nature-based interventions (NBIs) for children with autism spectrum disorder (ASD).ObjectiveTo systematically review and meta-analyze available evidence on the health-related outcomes in NBIs for children with ASD.Data SourcesThe Cumulative Index to Nursing and Allied Health Literature, Cochrane, Embase, Emcare, Education Resources Information Center, Global Health, MEDLINE, PsycInfo, SPORTDiscus, and Web of Science were searched from inception until May 2023. Google Scholar and references from included studies were searched for additional studies.Study SelectionIncluded studies were randomized clinical trials (RCTs), controlled studies, and single-group before-and-after studies that reported health-related outcomes.Data Extraction and SynthesisThis review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) reporting guidelines. Random-effects meta-analyses were used to synthesize the data. The findings of studies that were ineligible for meta-analysis were summarized according to the Synthesis Without Meta-analysis (SWIM) reporting guidelines.Main Outcomes and MeasuresThe outcomes of interest were health-related outcomes (ie, social functioning, behavioral functioning, emotional functioning, sensory functioning) and the self-reported well-being of children with ASD.ResultsA total of 24 studies with 717 participants (mean age range, 5.3 to 17.8 years; 141 [21.9%] female) were included. A meta-analysis from 13 studies indicated a significant negative moderate association between NBIs and social communication (standardized mean difference [SMD], −0.59; 95% CI, −0.85 to −0.34). For behavioral functioning outcomes, NBIs showed a significant moderate association with reduced hyperactivity (SMD, −0.56; 95% CI, −0.86 to −0.26) and a small to moderate association with reduced irritability (SMD, −0.49; 95% CI, −0.79 to −0.19). For sensory functioning, NBIs were significantly associated with improved inattention and distractibility (SMD, 1.13; 95% CI, 0.67 to 1.60). Significant moderate associations were observed in sensory seeking (SMD, 0.77; 95% CI, 0.33 to 1.22; P &amp;lt; .001; I2 = 0%) and sensory sensitivity (SMD, 0.56; 95% CI, 0.12 to 1.00; P = .01; I2 = 0%). Heterogeneity of the intervention effects was not high, and I2 ranged from 0% to 67%.Conclusions and RelevanceThe findings of this systematic review and meta-analysis suggested an association of NBIs in group-based recreational therapy with experiential learning with positive short-term outcomes on sensory, social, and behavioral functioning for children with ASD. Future evidence using robust study design to aid the health and functional trajectories of children with ASD is recommended.
Effectiveness of Aerobic Exercise and Tai Chi Interventions on Sleep Quality in Patients With Advanced Lung Cancer
Authors: Takemura Naomi , Cheung Denise Shuk Ting , Fong Daniel Yee Tak , Lee Anne Wing Mui , Lam Tai-Chung , Ho James Chung-Man , Kam Tsz Yeung , Chik Jeannie Yin Kwan , Lin Chia-Chin 
Published: December 2023

ImportanceSleep disturbances prevalent among patients with advanced lung cancer can aggravate physical and psychological symptoms, contributing to decreased quality of life and survival.ObjectiveTo compare the effectiveness of 2 physical activities of different modalities and intensities, namely aerobic exercise (AE) and tai chi (TC), on subjective sleep quality, physical and psychological outcomes, and survival in patients with advanced lung cancer.Design, Setting, and ParticipantsThis assessor-blinded, randomized clinical trial was conducted in 3 public hospitals in Hong Kong between December 19, 2018, and September 7, 2022. A total of 226 patients with advanced lung cancer were recruited and randomized 1:1:1 to AE, TC, or the control group.InterventionsFor 16 weeks, the AE group received two 60-minute supervised group exercise sessions and home-based exercises per month, and the TC group received 60-minute group sessions twice weekly. The control group received physical activity guidelines.Main Outcomes and MeasuresThe primary outcome was subjective sleep quality. Secondary outcomes included objective sleep measures, anxiety, depression, fatigue, quality of life, physical function, circadian rhythm, and 1-year survival. Assessments were conducted at baseline, 16 weeks (T1), and 1 year (T2).ResultsThe 226 participants had a mean (SD) age of 61.41 (8.73) years, and 122 (54.0%) were female. Compared with the control group, participants in the AE and TC groups showed statistically significant improvements in subjective sleep quality from baseline to T1 (AE: between-group difference, −2.72; 95% CI, −3.97 to −1.46; P &amp;lt; .001; TC: between-group difference, −4.21; 95% CI, −5.48 to −2.94; P &amp;lt; .001) and T2 (AE: between-group difference, −1.75; 95% CI, −3.24 to −0.26; P = .02; TC: between-group difference, −3.95; 95% CI, −5.41 to −2.49; P &amp;lt; .001), psychological distress, physical function, step count, and circadian rhythm. The TC group had a statistically significant greater improvement in sleep than the AE group at T1 (between-group difference, −1.49; 95% CI, −2.77 to −0.22; P = .02) and T2 (between-group difference, −2.20; 95% CI, −3.57 to −0.83; P &amp;lt; .001). Participants in the TC group showed statistically significant improvement in survival compared with the control group.Conclusions and RelevanceIn this randomized clinical trial, AE and TC improved sleep, psychological distress, physical function, and circadian rhythm, with TC demonstrating greater benefits on sleep and survival. Both exercises, but particularly TC, can be incorporated into lung cancer survivorship care.Trial RegistrationClinicalTrials.gov Identifier: NCT04119778
Living Seed Materials Made by Metal–Organic Framework‐Encapsulated Bacillus Subtilis Spore
Authors: Liu Yi , Wu Mengyao , Kang Anqi , Zhang Xiehaoran , Xie Ronghui , Huang Yuanpei , Huang Jiaofang , Chou Lien‐Yang , Zhong Chao 
Published: December 2023

AbstractGenetically modified microorganisms (GMMs) possess significant potential for a wide range of applications; however, their execution in real‐world scenarios, particularly in environments characterized by scarce nutrients and unpredictable conditions, proves daunting. Drawing inspiration from the adaptability of natural seeds, living seed materials that combine a core–shell structure ZIF‐8 and genetically engineered spores are introduced. By genetically modifying the Bacillus subtilis strain to express distinct fluorescent proteins, the visualization of spores within the hollow sphere is facilitated, allowing for differentiation between spore and bacteria states. Importantly, the ZIF‐8 shell exhibits responsiveness to diverse nutrient levels, providing enhanced environmental resistance in comparison to bare spores upon exposure to hypochlorous acid, UV‐C radiation, and even vacuum conditions. Moreover, the potential of living seed materials for living sensors of small molecules is showcased by elucidating that spores can be resuscitated into functional bacteria after release from ZIF‐8 material. This research establishes a novel approach for the general fabrication of Metal‐Organic Frameworks (MOF)‐based living spore hybrid materials, offering environmentally controlled release of living materials across a multitude of applications.
Remote‐Controllable Interfacial Electron Tunneling at Heterogeneous Molecular Junctions via Tip‐Induced Optoelectrical Engineering
Authors: Lee Jinhyoung , Kim Eungchul , Cho Jinill , Seok Hyunho , Woo Gunhoo , Yu Dayoung , Jung Gooeun , Hwangbo Hyeon , Na Jinyoung , Im Inseob , Kim Taesung 
Published: December 2023

AbstractMolecular electronics enables functional electronic behavior via single molecules or molecular self‐assembled monolayers, providing versatile opportunities for hybrid molecular‐scale electronic devices. Although various molecular junction structures are constructed to investigate charge transfer dynamics, significant challenges remain in terms of interfacial charging effects and far‐field background signals, which dominantly block the optoelectrical observation of interfacial charge transfer dynamics. Here, tip‐induced optoelectrical engineering is presented that synergistically correlates photo‐induced force microscopy and Kelvin probe force microscopy to remotely control and probe the interfacial charge transfer dynamics with sub‐10 nm spatial resolution. Based on this approach, the optoelectrical origin of metal–molecule interfaces is clearly revealed by the nanoscale heterogeneity of the tip‐sample interaction and optoelectrical reactivity, which theoretically aligned with density functional theory calculations. For a practical device‐scale demonstration of tip‐induced optoelectrical engineering, interfacial tunneling is remotely controlled at a 4‐inch wafer‐scale metal–insulator–metal capacitor, facilitating a 5.211‐fold current amplification with the tip‐induced electrical field. In conclusion, tip‐induced optoelectrical engineering provides a novel strategy to comprehensively understand interfacial charge transfer dynamics and a non‐destructive tunneling control platform that enables real‐time and real‐space investigation of ultrathin hybrid molecular systems.
Porous materials with suitable pore size and dual‐functional sites for benchmark one‐step ethylene purification
Authors: Wu Tangyin , Yu Cong , Krishna Rajamani , Qiu Zhensong , Pan Hanqian , Zhang Peixin , Suo Xian , Yang Lifeng , Cui Xili , Xing Huabin 
Published: December 2023

AbstractMultiple impurities removal represents one of the most daunting challenges in gas purification, but the attainment of efficient adsorptive separation is hindered by the difficulty in designing adsorbents that could simultaneously capture different impurities. Herein, we revealed a molecular trap within Zn‐trz‐ox (trz = 1,2,4‐triazole; ox = oxalic acid) that featured positive H and negative O sites, and the suitable pore size, which exhibited remarkable one‐step C2H4 purification performance directly from quaternary C2H4/C2H2/C2H6/CO2 mixtures. The separation selectivities of C2H4 with respect to CO2, C2H2, and C2H6 are 9.8, 2.6, and 2.5, higher than the sole adsorbent yet reported. Meanwhile, polymer grade C2H4 (≥99.95%) could be directly obtained with record C2H4 productivity of 1.5 mol kg−1, over 10 times higher than that of the previous benchmark material. The deep insight into the binding behavior revealed by simulation studies offers important clues for the design of advanced adsorbent for multiple impurities capture.
High‐Efficiency Ultraviolet Electroluminescence from Multi‐Resonance Phosphine Oxide Polycyclic Aromatics
Authors: Ma Peng , Chen Yingying , Man Yi , Qi Quan , Guo Yuanting , Wang Huiqin , Li Zhe , Chang Peng , Qu Chao , Han Chunmiao , Xu Hui 
Published: December 2023

AbstractEfficient ultraviolet (UV) electroluminescent materials remain a great challenge, since short peak wavelength <400 nm and narrow full width at half maximum (FWHM) <50 nm are simultaneously required. In this sense, multi‐resonance (MR) thermally activated delayed fluorescence (TADF) emitters featuring narrow‐band emissions hold the promise for UV applications. Herein, a novel MR‐TADF skeleton featuring carbazole‐phosphine oxide (P=O) fused aromatics is developed to construct the first two UV MR emitters named CzP2PO and tBCzP2PO. In addition to synergistic resonance effects of P=O and N atom, sp3‐hybrid P atom renders the curved polycyclic planes of CzP2PO and tBCzP2PO, giving rise to their narrowband UV emissions with peak wavelengths <390 nm and FWHM<35 nm. Besides configuration quasi‐planarization for radiation enhancement and quenching suppression, P=O moiety further enhances singlet‐triplet coupling to facilitate reverse intersystem crossing, resulting in the state‐of‐the‐art photoluminescence quantum yield of 62 % in tBCzP2PO doped films. As consequence, tBCzP2PO endowed its UV organic light‐emitting diodes with the peak at 382 nm and FWHM of 32 nm, and especially the record‐high external quantum efficiency (EQE) of 15.1 % among all kinds of UV devices. Our results demonstrate great potential of P=O based MR emitters in practical applications including optoelectronics, biology and medicine science.
Shellac‐based drug delivery system with controlled drug‐release and tunable intragastric floating‐retention properties
Authors: Yuan Ziting , Li Shizhen , Sun Bin , Li Kai , Tang Wei , Xu Juan 
Published: December 2023

AbstractIntragastric floating‐retention drug delivery system (IFRDDS) could effectively prolong drug‐releasing time, achieving a good clinical efficacy of encapsulated drug. In this research, the green amphiphilic resin, shellac was adopted to fabricate a continuous network as the matrix of IFRRDS by their self‐assembly behavior triggered by H+. Meanwhile, the NaHCO3 and hydroxypropyl methyl cellulose (HPMC) acted as a forming agent and a precipitating agent to control the floating behavior, respectively, which could create a porous structure for lowering the true density of the tablet and continuously absorb water to enhance the density of tablet, respectively. Interestingly, the synergistic effect of NaHCO3 and HPMC was beneficial to the initiation of the floating of the complex tablet. By regulating tablet's constitution, we could tune the floating lag time, floating time, and drug‐release behavior of the tablet. For example, the drug‐releasing curve of the shellac‐based tablet containing 3 wt% HPMC and 10 wt% NaHCO3 presented a typical linear model, which was an ideal linear drug‐releasing system, and could float in 3 min and last for 16 h. Therefore, we successfully employed a facile strategy to fabricate a biobased IFRRDS with controlled drug‐release and tunable intragastric floating‐retention properties, which has great potential for the advanced medical industry.
Magnetic nanocomposite hydrogel with ultra‐stretchable as strain sensors for monitoring human motion and the change of magnetic field
Authors: Cao Qinglong , Shu Zhen , Zhang Taoyi , Ji Wenxi , Chen Jing , Wei Yun 
Published: December 2023

AbstractIn recent years, the smart hydrogels have gained much concern in the field of research specially related to flexible strain sensors because they exhibit many types of smart interactions that can be useful in wearable devices. However, the conventional hydrogels have poor electrical conductivity that affect the performance of the sensors, so it remains a challenge to achieve noncontact signal monitoring (e.g., for the detection of magnetic field changes). In this study, an ultra‐stretchable and magnetically responsive conductive hydrogel was fabricated by adding magnetic ferric tetroxide@polypyrrole composite nanoparticles (Fe3O4@PPy NPs) to polyacrylamide (PAm). The nanoparticles were easily agglomerated and improved the compatibility of PPy and hydrogel. The obtained PAm/Fe3O4@PPy hydrogel showed an ultra‐stretchability of (961%), a low elastic modulus of (87.8 kPa), and an excellent toughness of (1010.5 kJ m−3). Moreover, PAm/Fe3O4@PPy hydrogel also exhibited a high electrical conductivity of 0.34 S m−1, and the PAm/Fe3O4@PPy hydrogel sensor could detect human motions (such as bending of finger, bending of wrist) and muscle micromotion (such as pronouncing). In addition, it can also monitor the change in magnitude of magnetic field.
Knee cartilage change on magnetic resonance imaging: Should we lump or split topographical regions? A 2‐year study of data from the osteoarthritis initiative
Authors: Richard Michael J. , Lo Grace H. , Driban Jeffrey B. , Canavatchel Amanda R. , LaValley Michael , Zhang Ming , Price Lori Lyn , Miller Eric , Eaton Charles B. , McAlindon Timothy E. 
Published: December 2023

AbstractObjectiveWe challenge the paradigm that a simplistic approach evaluating anatomic regions (e.g., medial femur or tibia) is ideal for assessing articular cartilage loss on magnetic resonance (MR) imaging. We used a data‐driven approach to explore whether specific topographical locations of knee cartilage loss may identify novel patterns of cartilage loss over time that current assessment strategies miss.DesignWe assessed 60 location‐specific measures of articular cartilage on a sample of 99 knees with baseline and 24‐month MR images from the Osteoarthritis Initiative, selected as a group with a high likelihood to change. We performed factor analyses of the change in these measures in two ways: (1) summing the measures to create one measure for each of the six anatomically regional‐based summary (anatomic regions; e.g., medial tibia) and (2) treating each location separately for a total of 60 measures (location‐specific measures).ResultsThe first analysis produced three factors accounting for 66% of the variation in the articular cartilage changes that occur over 24 months of follow‐up: (1) medial tibiofemoral, (2) medial and lateral patellar, and (3) lateral tibiofemoral. The second produced 20 factors accounting for 75% of the variance in cartilage changes. Twelve factors only involved one anatomic region. Five factors included locations from adjoining regions (defined by the first analysis; e.g., medial tibiofemoral). Three factors included articular cartilage loss from disparate locations.ConclusionsNovel patterns of cartilage loss occur within each anatomic region and across these regions, including in disparate regions. The traditional anatomic regional approach is simpler to implement and interpret but may obscure meaningful patterns of change.
‘We like because we feel liked’ exposure to cultural fusion improves outgroup attitudes through contact metacognition
Authors: Bao Yan , Wang Weichao , Zhao Yufang , Yu Dongfang , Willie Njoroge , Chen Bing 
Published: December 2023

AbstractBased on the common ingroup identity model and the principle of group reciprocity, this study proposes that exposure to cultural fusion can improve outgroup attitudes by activating positive contact metacognition, that is, the subjective perception of the outgroup's desire for contact with the ingroup. To test this hypothesis, we conducted three experiments with Chinese Yi students. They were randomly primed with Dai‐Yi cultural fusion pictures, Dai cultural pictures, Yi cultural pictures or geometry pictures (Dai and Yi are two Chinese ethnic minorities). They were asked to report their contact metacognition, cultural identity (Experiments 2 and 3), explicit (willingness to help; Experiments 1 and 2) and implicit attitudes towards Dai (SC‐IAT; Experiment 3). The results found that exposure to Dai‐Yi cultural fusion improved contact metacognition among Yi participants, subsequently leading to more positive explicit and implicit attitudes towards Dai. Unexpectedly, cultural identity did not moderate the mediated effect of contact metacognition. These findings suggest that exposure to cultural fusion is an effective and stable strategy for fostering harmonious intergroup relationships and specifically highlight the importance of contact metacognition. Please refer to the Supplementary Material section to find this article's Community and Social Impact Statement.
Influence of clay impurities on the performance of calcined clay‐limestone cements
Authors: Matschei Thomas , Muzenda Tafadzwa Ronald , Georget Fabien 
Published: December 2023

AbstractThe use of blended cements including novel supplementary cementitious materials is the most powerful lever to cut down the carbon footprint of cementitious materials. This has led to an increased interest in research on calcined clay limestone cement (CCLC) over the last years. In this study, we investigate the fresh and early hydration properties of CCLC using kaolinitic clays with a wide range of chemical and mineralogical compositions, which are all of industrial origin. Results show that early performance of calcined clays is not only dependent on kaolinite content but that the total specific surface area of the raw and calcined clays plays a key role in the onset and evolution of rheological properties as well as early hydration and reactivity. Further analysis indicated that among secondary mineral impurities, iron phases affect the physiochemical properties of calcined clays. This is shown by the high specific surface area of these clays. It appears possible that by causing higher kaolinite structure disorder, iron phases also impact hydration.
Spatially resolved in situ analyses of the adsorption behavior of different admixtures on binders
Authors: Kosenko Denis , Wetzel Alexander , Middendorf Bernhard 
Published: December 2023

AbstractAdmixtures play a key role in improving the performance of the cement paste matrix. A more efficient matrix can allow a reduction the binder content while maintaining the same properties. There is currently a lack of knowledge about the spatial distribution of admixtures in situ on binder surfaces which would facilitate the use of admixtures in general in inorganic binder systems. Imaging techniques such as confocal laser scanning microscopy (CLSM) and fluorescence microscopy can be used to study the spatiotemporal adsorption of superplasticizers and plasticizers on binder particles. However, in order to make the admixtures visible using these methods, they must first be coupled to a fluorescent molecule (staining). Different types of superplasticizers and plasticizers, including polycarboxylate ethers (PCE) and lignosulfonate (LS) were stained and tested. It was shown that not only adsorption but also desorption can be observed and that desorption cannot play a relevant role in a binder system. The absence of desorption could be used to observe a real system in permanent preparations, so that the adsorption on individual cement grains could be followed in a time‐dependent manner. The newly developed method provides unprecedented insight into the adsorption of additives.