bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and application of a competitive ELISA for the detection of antibodies against
Salmonella
Abortusequi in equids
Authors: Guo Kui , Guo Wei , Liu Diqiu , Zhang Weiguo , Yang Yan , Zhang Zenan , Li Shuaijie , Wang Jinhui , Chu Xiaoyu , Wang Yaoxin , Hu Zhe , Wang Xiaojun 
Published: October 2023

ABSTRACT

The high abortion rate associated with
Salmonella
Abortusequi (
S
. Abortusequi) infection in equids has re-emerged over the past 10 years and has caused serious economic losses to China. Our previous studies showed that the flagellin FljB gene could distinguish
S
. Abortusequi from most
Salmonella
serotypes. In this study, the flagellin antigen was used to develop a competitive enzyme-linked immunosorbent assay (cELISA) that could be used to detect both horse and donkey serum samples using a monoclonal antibody (MAb) that was found to bind to FljB. A cELISA was established using the purified MAb coating of the plate and incubation of the mixture of horseradish peroxidase (HRP)-conjugated FljB antigen with the undiluted serum sample. The performance of the cELISA and the tube agglutination test (TAT) assay was compared with respect to sensitivity and specificity, by testing a panel containing 660
S
. Abortusequi-positive and 515
S
. Abortusequi-negative serum samples, all of which had been characterized by Western blotting. Receiver operator characteristic (ROC) analyses were performed to determine the cutoff value and estimate the detection specificity (Sp) and sensitivity (Se). ROC analysis showed that the area under the ROC curve (AUC) values of cELISA [AUC = 0.9941; 95% confidence interval (CI), 0.9898–0.9984] were higher than those of TAT (AUC = 0.7705; 95% Cl, 0.7437–0.7972). A cutoff value of 39.5% was selected with Sp and Se values of 100 (95% Cl, 99.26–100.00) and 97.58 (95% Cl, 96.10–98.50), respectively. The cELISA has excellent futures compared with TAT, such as shortened detection time, no need for pre-treatment of sera, and easy interpretation of the results, and is more suitable for disease surveillance.
Law enforcement and firearms: understanding firearm ownership and storage habits
Authors: Bond Allison E , Stanley Ian , Bandel Shelby L , Anestis Michael 
Published: October 2023

ObjectiveThis study seeks to better understand firearm ownership among law enforcement officers (LEO), with the goal of informing future firearm injury and suicide prevention efforts. We describe the frequency and sociodemographic correlates of firearm ownership and storage practices among, and examine the association between suicidal ideation and current firearm storage practices.MethodsThe present study used data from a large online study (n=6410) and included data from individuals who were currently or previously being employed as an LEO (n=369;M(SD) age=39.2 y (15.8 y), 75.2% male, 66.7% white). Self-report measures were used to assess for firearm ownership, storage habits and suicidal ideation. Descriptive statistics were used to describe the frequency of firearm ownership and logistic regressions were used to examine the extent to which demographic characteristics and suicidal ideation were associated with firearm ownership.ResultsOverall, 70.5% (n=261) of the sample reported firearm ownership. LEO who were older had significantly lower odds of reporting firearm ownership. Those who were married and those who reported lifetime suicidal ideation had significantly greater odds of reporting firearm ownership. Whereas firearm-owning LEO who reporting storing a firearm locked had significantly lower odds of reporting lifetime suicidal ideation, those who reported storing a firearm unloaded had significantly greater odds of reporting lifetime suicidal ideation.ConclusionFindings have important public health implications and can be used to increase adherence with secure storage recommendations. Increasing secure storage may help reduce suicide risk among LEO, a sample at heightened risk for suicide.
Detailed clinical, physiological and pathological phenotyping can impact access to disease-modifying treatments in ATTR carriers
Authors: Beauvais Diane , Labeyrie Céline , Cauquil Cécile , Francou Bruno , Eliahou Ludivine , Not Adeline , Echaniz-Laguna Andoni , Adam Clovis , Slama Michel S , Benmalek Anouar , Leonardi Luca , Rouzet François , Adams David , Algalarrondo Vincent , Beaudonnet Guillemette 
Published: October 2023

BackgroundHereditary transthyretin amyloidosis is a life-threatening autosomal dominant systemic disease due to pathogenicTTRvariants (ATTRv), mostly affecting the peripheral nerves and heart. The disease is characterised by a combination of symptoms, organ involvement and histological amyloid deposition. The available disease-modifying ATTRv treatments (DMTs) are more effective if initiated early. Pathological nerve conduction studies (NCS) results are the cornerstone of large-fibre polyneuropathy diagnosis, but this anomaly occurs late in the disease. We investigated the utility of a multimodal neurological and cardiac evaluation for detecting early disease onset in ATTRv carriers.MethodsWe retrospectively analysed a cohort of ATTRv carriers with normal NCS results regardless of symptoms. Multimodal denervation and infiltration evaluations included a clinical questionnaire (Lauria and New York Heart Association (NYHA)) and examination, intra-epidermal nerve fibre density assessment, autonomic assessment based on heart rate variability, Sudoscan, meta-iodo-benzyl-guanidine scintigraphy, cardiac biomarkers, echocardiography, MRI and searches for amyloidosis on skin biopsy and bone scintigraphy.ResultsWe included 130 ATTRv carriers (40.8% men, age: 43.6±13.5 years), with 18 amyloidogenicTTRgene mutations, the majority of which was the late-onset Val30Met variant (42.3%). Amyloidosis was detected in 16.9% of mutation carriers, including 9 (6.9%) with overt disease (Lauria>2 or NYHA>1) and 13 asymptomatic carriers (10%) with organ involvement (small-fibre neuropathy or cardiomyopathy). Most of these patients received DMT. Abnormal test results of unknown significance were obtained for 105 carriers (80.8%). Investigations were normal in only three carriers (2.3%).ConclusionsMultimodal neurological and cardiac investigation of TTRv carriers is crucial for the early detection of ATTRv amyloidosis and initiation of DMT.
Effect of cold winters on the risk of new asthma: a case-crossover study in Finland
Authors: Belachew Abate Bekele , Rantala Aino K , Jaakkola Maritta S , Hugg Timo T , Ruuhela Reija , Kukkonen Jaakko , Jaakkola Jouni J K 
Published: October 2023

BackgroundCold weather increases respiratory symptoms and provokes exacerbations of asthma, but there are no previous studies on its role in the aetiology of asthma.ObjectiveWe tested the hypothesis that a cold winter increases the risk of developing asthma during the following 1 to 2 years.MethodsWe conducted a case-crossover study of 315 newly diagnosed cases of asthma from the population-based Espoo Cohort Study from birth to the age of 27 years. The hazard period constituted 3 winter months preceding the onset of asthma and bidirectional reference periods of 1 year before hazard period and 1 year after onset of asthma. Exposure constituted average ambient temperature during the winter months of December, January and February. The outcome of interest was new doctor-diagnosed asthma. The measure of effect was OR of asthma estimated by conditional logistic regression analysis.ResultsThe average winter temperature for the study period from winter 1983 to 2010 was −4.4°C (range −10.7 to 0.4). A 1°C decrease in the average winter temperature predicted a 7% increase in the risk of new asthma (OR=1.07, 95% CI 1.02 to 1.13). A cold winter with an average temperature below the climate normal value (−4.5°C; period 1981–2010) increased the risk of new asthma by 41% during the following year (OR: 1.41; 95% CI 1.04 to 1.90).ConclusionsThis case-crossover study provides original evidence that a cold winter with below normal average temperatures increases the risk of developing new asthma during the following 1 to 2 years.
Extracting the energy and angular momentum of a Kerr black hole
Authors: Rueda J. A. , Ruffini R. 
Published: October 2023

AbstractIt has been thought for decades that rotating black holes (BHs) power the energetic gamma-ray bursts (GRBs) and active galactic nuclei (AGNs), but the mechanism that extracts the BH energy has remained elusive. We here show that the solution to this problem arises when the BH is immersed in an external magnetic field and ionized low-density matter. For a magnetic field parallel to the BH spin, the induced electric field accelerates electrons outward and protons inward in a conical region, centered on the BH rotation axis, and of semi-aperture angle $$\theta \approx 60^\circ $$

θ
≈

60
∘

from the BH rotation axis. For an antiparallel magnetic field, protons and electrons exchange their roles. The particles that are accelerated outward radiate off energy and angular momentum to infinity. The BH powers the process by reducing its energy and angular momentum by capturing polar protons and equatorial electrons with net negative energy and angular momentum. The electric potential allows for negative energy states outside the BH ergosphere, so the latter does not play any role in this electrodynamical BH energy extraction process.
Taxonomic survey on the genus Limonium in the Maltese islands, with description of a new species
Authors: AGIUS DOLORES RITA , GALEA MARIA ELIZABETH , CAMBRIA SALVATORE , GALDO GIANPIETRO GIUSSO DEL , BRULLO SALVATORE 
Published: October 2023

The native species of the genus Limonium (Plumbaginaceae) occurring in the Maltese Islands are investigated. According to current literature, this genus is hitherto represented in this territory by L. virgatum, L. zeraphae and L. melitense, but recent field surveys led to the discovery of a new species growing exclusively on the islet of Cominotto and described as L. lanfrancoi. The latter, for some morphological features regarding the vegetative and reproductive traits, shows closer relationships with L. melitensis, from which, however, it differs in several diacritical characters. For each species, a detailed illustration and a new morphological description based on rich herbarium materials are provided, as well as information on their typification, synonymy, phenology, ecology, distribution, conservation status and a list of examined herbarium specimens is given. Besides, an updating analytical key has been elaborated.
The Predatory Paradox
Authors: Koerber Amy , Starkey Jesse C. , Ardon-Dryer Karin , Cummins R. Glenn , Eko Lyombe , Kee Kerk F. 
Published: October 2023

In today’s ‘publish or perish’ academic setting, the institutional prizing of quantity over quality has given rise to and perpetuated the dilemma of predatory publishing. Upon a close examination, however, the definition of ‘predatory’ itself becomes slippery, evading neat boxes or lists which might seek to easily define and guard against it. This volume serves to foreground a nuanced representation of this multifaceted issue. In such a rapidly evolving landscape, this book becomes a field guide to its historical, political, and economic aspects, presenting thoughtful interviews, legal analysis and original research. Case studies from both European-American and non-European-American stakeholders emphasize the worldwide nature of the challenge faced by researchers of all levels.
This coauthored book is structured into both textual and supplemental materials. Key takeaways, discussion questions, and complete classroom activities accompanying each chapter provide opportunities for engagement and real-world applications of these concepts.
Crucially relevant to early career researchers and the senior faculty, library scholars, and administrators who mentor and support them, 'The Predatory Paradox: Ethics, Politics, and Practices in Contemporary Scholarly Publishing' offers practical recommendations for navigating the complex and often contradictory advice currently available. University instructors and teaching faculty will also find the reading essential in order to properly prepare both graduate and undergraduate students for the potential pitfalls endemic to scholarly publishing.
Nonprofit-business collaboration: An empirical-based framework for value creation
Authors: Al-Tabbaa Omar 
Published: October 2023

While interest in nonprofit-business collaboration (NBC) has grown rapidly in the past decades, literature has underexposed the peculiarities of nonprofit organizations (NPOs), compared to the ample attention on the perspective of the firm and the implications for society. In specific, we lack clarity on how NPOs can deliberately seek collaboration with the business sector to foster their organizational viability and economic interests, in addition to their traditional focus on delivering social value and meaningfulness. This active approach has become imperative given the growing uncertainty of government funding and budgetary constraints. However, the proactive involvement in NBC is complex and risky. In this study, we address this dilemma by investigating several NPOs that are active in NBC. Based on the analysis and findings, we developed an empirical-based framework that explains the essence of NPOs’ proactive engagement with the business sector. More specifically, we offer subtle theoretical and empirical investigations of various internal and external contingencies that explain the effectiveness of NPOs in establishing collaboration with firms for value creation. Moreover, we advance the current debate concerning the need to establish a clear distinction between value creation for society (conceived as the sum of benevolent benefits obtained from the collaboration) and for the partners. As such, we showed that the active-in-collaboration NPOs distinguish between different NBC values that include economic benefits (for survival), institutional development (for organizational effectiveness), and transformational for changing business practice (for the fulfillment of mission). Therefore, we shift the focus from the predominating perspectives of society and business to consider NPOs as an active component of this relationship. Together, these findings offer a robust nonprofit-centric foundation that is necessary for NBC practice and theory development. JEL Classification: M1 Business Administration
Differential emotional expression in autobiographical narratives: The case of Arabic–English bilinguals
Authors: El-Dakhs Dina Abdel Salam , Ahmed Mervat M. , Altarriba Jeanette , Sonbul Suhad 
Published: October 2023

Aims and Objectives: This study aimed to examine how bilinguals express their emotions in their first language (L1) versus their second language (L2) in autobiographical narratives. Methodology: A total of 64 Egyptian learners of English as a foreign language (EFL) took an English language proficiency test, and wrote about the happiest or saddest experiences they recalled in Arabic and in English. Data and Analysis: The narratives were analysed for the frequency and the valence of emotion words. Findings/Conclusions: The results showed no difference between the narratives of the two languages in terms of the frequency of words. However, the L2 narratives were generally more negatively valenced than the L1 narratives, and more emotional polarity was noted in the L2 narratives, particularly in sad situations. The results failed to show any effect for the participants’ age, gender or L2 proficiency on the frequency or valence of the emotion words. Originality: This study compared two languages that are distant at several levels, including typography and culture. In addition, the study examined some variables that were not investigated in earlier emotion studies of bilingualism. Significance/Implications: The study presents several implications in relation to the emotional contexts of learning theory and the pedagogy of emotion words.
Determination of bilingualism subtypes and their relationship with linguistic abilities in Lebanese bilinguals
Authors: Kassir Rania , Abboud Halim , Godefroy Olivier 
Published: October 2023

Aims and objectives: Profiles of bilinguals vary among studies due to the diversity of intrinsic and extrinsic factors, bilingualism classification and to the discrepancies between measures. Thus, a generalizable index with a defined threshold is needed to capture the linguistic dominance of bilinguals and facilitate comparisons between studies. This study’s objective was to define and examine the validity of a new bilingualism index. Methodology: This index was derived from the Language Experience and Proficiency Questionnaire (LEAP-Q) conducted on 100 bilingual Lebanese participants (age = 68.2 ± 9.4 years; education = 12.8 ± 5.2 years). Data and analysis: The bilingualism index was based on selected items of the LEAP-Q, optimizing the explained variance on a linear regression using the differential Arabic-French score on the Boston Naming Test as the dependent variable. The validity of the classification was examined using other linguistic (articulatory rate, shortened Token test, Stroop reading subtest) and cognitive screening tests (mini-mental state examination [MMSE]). Findings and conclusion: LEAP speaking and oral comprehension scores provided a parsimonious index that accounted for naming variance ( R2 = .435, p = .0001) and subdivide our population into three bilingualism subgroups (prominent Arabic, balanced, prominent French). A prominent language advantage was found in the expected direction on linguistic (articulatory rate: p = .03; shortened Token test: p = .026; Stroop reading subtest: p = .0001) and cognitive screening tests (MMSE: p = .08). Originality and implications: These results show that a simple index can accurately characterize adult bilingualism subtypes and offers clinicians an easy and fast tool compared with the usual procedure used to determine individuals and patient’s bilingualism subtype. More broadly, the index’s validity in other bilingual populations is warranted for generalizability of the present findings.
Association between three-dimensional motion analysis of the thumb and clinical parameters in patients with carpal tunnel syndrome
Authors: Kodama Akira , Kurumadani Hiroshi , Tanaka Teruyasu , Shinomiya Rikuo , Sunagawa Toru , Adachi Nobuo 
Published: October 2023

We analysed the relationship between motor dysfunction of the thumb and the clinical parameters of carpal tunnel syndrome using three-dimensional motion analysis. This single-centred, prospective study included 65 hands in 51 patients with idiopathic carpal tunnel syndrome and 30 healthy hands. Three-dimensional thumb kinematics were acquired using a motion capture system with a retroreflective surface-based marker method. The trajectory area of thumb tip, adduction and abduction of the trapeziometacarpal joints and metacarpophalangeal joints were correlated with the clinical parameters. There was no significant correlation between the results of motion analysis values and patient-reported outcomes measures. Thumb movement disorder associated with carpal tunnel syndrome affected specific activities of daily living based on the pinching movements, such as ‘writing’ and ‘buttoning clothes’ among the patient-reported outcome measure items. Level of evidence: III
Intra-individual qualitative and quantitative comparison of [68Ga]Ga-DOTATATE PET/CT and PET/MRI
Authors: Lens Géraldine , Ahmadi Bidakhvidi Niloefar , Vandecaveye Vincent , Grauwels Steven , Laenen Annouschka , Deckers Wies , Peeters Ronald , Dresen Raphaëla C. , Dekervel Jeroen , Verslype Chris , Nackaerts Kristiaan , Clement Paul M. , Van Cutsem Eric , Koole Michel , Goffin Karolien , Van Laere Koen , Deroose Christophe M. 
Published: October 2023

Background: Somatostatin receptor (SSTR) positron emission tomography (PET) is a cornerstone of neuroendocrine tumor (NET) management. Hybrid PET/magnetic resonance imaging (MRI) is now available for NET-imaging, next to PET/computed tomography (CT). Objectives: To determine whether CT or MRI is the best hybrid partner for [68Ga]Ga-DOTATATE PET. Design: Monocentric, prospective study. Methods: Patients received a same-day [68Ga]Ga-DOTATATE PET/CT and subsequent PET/MRI, for suspicion of NET, (re)staging or peptide receptor radionuclide therapy-selection. The union (PETunion) of malignant lesions detected on PETCT and PETMRI was the reference standard. Concordance of detection of malignant lesions in an organ was measured between PETunion and CT and PETunion and MRI. Seven bins were used to categorize the number of malignant lesions, containing following ordinal variables: 0, 1, 2–5, 6–10, 11–20, >20 countable and diffuse/uncountable. The difference in number of malignant lesions was obtained as the difference in bin level (‘Δbin’) between PETunion and CT and PETunion and MRI with a Δbin closer to zero implying a higher concordance rate. Results: Twenty-nine patients were included. Primary tumors included 17 gastroenteropancreatic-NETs, 1 colon neuroendocrine carcinoma, 7 lung-NETs and 2 meningiomas. Patient level concordance with PETunion was 96% for MRI and 67% for CT ( p = 0.039). Organ level concordance with PETunion was 74% for MRI and 40% for CT ( p < 0.0001). In bone, there was a higher concordance rate for MRI compared to CT, 92% and 33%, respectively ( p = 0.016). Overall, a mean Δbin of 0.5 ± 1.1 for PETunion/MRI and 1.4 ± 1.2 for PETunion/CT ( p < 0.0001) was noted. In liver, a mean Δbin of 0.0 ± 1.1 for PETunion/MRI and 1.7 ± 1.2 for PETunion/CT was observed ( p = 0.0078). In bone, a mean Δbin closer to zero was observed for PETunion/MRI compared to PETunion/CT, 0.6 ± 1.4 and 2.0 ± 1.5, respectively ( p = 0.0098). Conclusions: Compared to SSTR PET/CT, SSTR PET/MRI had a higher patient and organ level concordance for malignant tumoral involvement and number of malignant lesions, with a clear added value in bone and liver specifically.
BMP7 alleviates trigeminal neuralgia by reducing oligodendrocyte apoptosis and demyelination
Authors: Chen Kai , Wei Xiaojin , Wang Ruixuan , Yang Lin , Zou Dingquan , Wang Yaping 
Published: October 2023

Abstract
Background
BMP7 has been shown to have neuroprotective effects and to alleviate demyelination. However, its role in trigeminal neuralgia (TN) has not been well investigated. The current study aims to determine whether BMP7 plays a role in demyelination, its effects on pain behaviors and mechanism of action in rats with TN.

Methods
We used an infraorbital-nerve chronic-constriction injury (ION-CCI) to establish a rat model of TN. Adeno-associated viruses (AAVs) were injected into the rats to upregulate or downregulate BMP7. The mechanical withdrawal thresholds (MWT) of the injured rats were detected using Von Frey filaments. The changes in expression levels of BMP7 and oligodendrocyte (OL) markers were examined by western blotting, quantitative real-time PCR, immunofluorescence, and transmission electron microscopy.

Results
The ION-CCI induced mechanical allodynia, demyelination, and loss of OLs with a reduction of BMP7. Short-hairpin RNA (shRNA)-BMP7 that inhibited BMP7 expression also caused mechanical allodynia, demyelination, and loss of OLs, and its mechanism may be OL apoptosis. Overexpressing BMP7 in the trigeminal spinal subnucleus caudalis(VC) with AAV-BMP7 relieved all three phenotypes induced by the CCI, and its mechanism may be alleviating OLs apoptosis. Two signal pathways associated with apoptosis, STAT3 and p65, were significantly downregulated in the VC after CCI and rescued by BMP7 overexpression.

Conclusion
BMP7 can alleviate TN by reducing OLs apoptosis and subsequent demyelination. The mechanism behind this protection could be BMP7-mediated activation of the STAT3 and NF-κB/p65 signaling pathway and subsequent decrease in OL apoptosis. Importantly, our study presents clear evidence in support of BMP7 as a possible therapeutic target for the treatment of TN.