bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerebral embolic protection during transcatheter stent expansion of restrictive extra-cardiac Fontan conduit
Authors: Haddad Raymond N. , Naggara Olivier , Bonnet Damien , Malekzadeh-Milani Sophie 
Published: November 2023

AbstractWe report a 20-year-old female patient (76 Kg/164 cm) with an extra-cardiac Fontan circulation who was referred to our institution for exertional dyspnoea and desaturation. The patient was diagnosed with a large calcified thrombus at the level of the Fontan fenestration, protruding inside the lumen of the conduit and reducing the diameter by half with a 3 mmHg pressure gradient. Transcatheter stent expansion of the obstructed extra-cardiac conduit was done with a 48 mm long XXL PTFE-covered Optimus-CVS® under temporary cerebral embolic protection with a TriGUARD-3™ deflection filter device (Keystone Heart). There was no procedural complication and the 3 months clinical outcomes are good.
Cardiac MRI six months after the onset of multisystem inflammatory syndrome in children and adolescents temporally related to COVID-19: A retrospective follow-up study
Authors: Karas Thomas , Scala Mario , Biebl Ariane , Steiner Jürgen , Fellner Franz , Tulzer Gerald , Tulzer Andreas 
Published: November 2023

AbstractBackground:In 2020, reports revealed cases called multisystem inflammatory syndrome in children and adolescents temporally related to COVID-19 or multisystem inflammatory syndrome in children. A small proportion of patients suffer from persistent left ventricular dysfunction at discharge. The primary aim was to investigate if myocardial impairment persists during follow-up in these patients.Methods:Children fulfilling the criteria for multisystem inflammatory syndrome in children with cardiac involvement hospitalized between December 2020 and February 2022 were included in this retrospective single centre study. Cardiac MRI was performed six months after the onset of symptoms to evaluate possible persistent myocardial damage.Results:Fifteen patients (80% male) with a median age of 8 years (interquartile range 4.5 - 13.5 years) were included. Upon admission, eight patients (53%) presented with reduced left ventricular function, with a median left ventricular ejection fraction of 54% (interquartile range 49.5%-61.5%) on transthoracic echocardiography. Elevated levels of cardiac-specific markers were found in 14 patients (93%). Cardiac MRI was performed in 12 patients at a median of 190.5 days after the onset of symptoms. Nine patients (75%) had normal left ventricular function, with a median left ventricular ejection fraction of 59.45%, while the remaining patients showed mildly to moderately reduced values. None of the patients showed signs of late gadolinium enhancement, indicating the absence of persistent myocardial scarring.Conclusion:During a follow-up of 6.2 months, mild to moderate cardiac impairment was revealed in 25% of patients evaluated by cardiac MRI. Although a majority of patients do not show signs of cardiac impairment, close follow-ups should be performed in a proportion of patients.
Cladistics groupings of the active breeding cocoa genetic resources of Nigeria for physicochemical and nutraceutical traits
Authors: Adewale Daniel B. , Adeigbe Oluwayemisi O. 
Published: November 2023

AbstractPreference for functional and nutritious food capable of meeting consumers' demand and health is on the increase. The present preliminary study seeks to assess physico-chemical and nutraceutical diversity in the cocoa bean powder of 77 genotypes present in four Nigerian cocoa field banks. Twenty ripe pods/genotypes in each of the four active breeding field banks at the Cocoa Research Institute of Nigeria (CRIN), Ibadan, Nigeria were utilized. Composite beans from the 20 pods of each genotype were singly fermented, sun-dried and milled. Duplicate samples of the powder of each genotype were analysed for physico-chemical and nutraceutical components. Twenty-one polymorphic variables distinguished the 77 cocoa genotypes. Grouping by dendogram identified four clusters, three differently and uniquely captured 100% of the genotype membership in the local clone, international clone and the regional varieties field bank but 86% of the genotypes in the hybrid trial field bank were grouped in cluster I. Prominent traits with highest values in each clusters were: protein, pH, Ca, K and Fe (Cluster I), Zn and Mg (Cluster II), crude fat and P (Cluster III) and crude fibre, ash, theobromine, flavonoids and caffeine (Cluster IV). Exploitable diversity for nutritional quality improvement is present in the active breeding and working collections of Nigerian cocoa field banks.
A scientometric review of the peer-reviewed research on high tunnels in the United States
Authors: Donovan Megan , Ruiz-Menjivar Jorge , Coolong Timothy , Swisher Marilyn E. 
Published: November 2023

Abstract
This study assessed the extant literature related to the use of high tunnels (HTs) in agricultural systems in the United States since the 2009 launch of the Natural Resources Conservation Service (NRCS) High Tunnel Initiative. This NRCS program led to an increase in HT adoption nationwide. The literature searches were conducted using the Web of Science (WoS) database. The final sample was 133 peer-reviewed articles published between 2009 and February 2023. We used CiteSpace 6.2.R1 and Gephi 0.9.2 to conduct co-citation, co-author, co-institution, and clustering techniques. The findings showed that the peer-reviewed literature about HT use has increased since 2009, substantially rising between 2017 and 2021. Horticulture was the top subject category in the literature, and most articles were published in peer-reviewed journals of the American Society for Horticultural Science (i.e., HortTechnology and HortScience). The research field evolved from general HT practices, nutrient management, and plant pathology to focus on trials of specific crops and integrated pest management. The institutions with the most contributions to the HT literature were Kansas State University, the University of Florida, Michigan State University, Purdue University, and the University of Minnesota. The patterns of HT research revealed in this study offer a greater understanding of the current state of knowledge to inform the focus of future research.
Subseasonal Prediction of Impactful California Winter Weather in a Hybrid Dynamical‐Statistical Framework
Authors: Guirguis Kristen , Gershunov Alexander , Hatchett Benjamin J. , DeFlorio Michael J. , Subramanian Aneesh C. , Clemesha Rachel , Delle Monache Luca , Ralph F. Martin 
Published: November 2023

AbstractAtmospheric rivers (ARs) and Santa Ana winds (SAWs) are impactful weather events for California communities. Emergency planning efforts and resource management would benefit from extending lead times of skillful prediction for these and other types of extreme weather patterns. Here we describe a methodology for subseasonal prediction of impactful winter weather in California, including ARs, SAWs and heat extremes. The hybrid approach combines dynamical model and historical information to forecast probabilities of impactful weather outcomes at weeks 1–4 lead. This methodology uses dynamical model information considered most reliable, that is, planetary/synoptic‐scale atmospheric circulation, filters for dynamical model error/uncertainty at longer lead times and increases the sample of likely outcomes by utilizing the full historical record instead of a more limited suite of dynamical forecast model ensemble members. We demonstrate skill above climatology at subseasonal timescales, highlighting potential for use in water, health, land, and fire management decision support.
Coastal Mountains Amplified the Impacts of Orbital Forcing on East Asian Climate in the Late Cretaceous
Authors: Zhang Jian , Flögel Sascha , Hu Yongyun , Zhao Anni , Chu Runjian , Zhu Chenguang , Wang Chengshan 
Published: November 2023

AbstractDuring the Cretaceous, there were two factors that had important influences on the East Asian climate, the East Asian coastal mountains and Earth's orbital cycling. An important question is how the coastal mountains modulated the variability of East Asian climate over orbital timescales. Here, we perform simulations with the coastal mountains of 0, 2, and 4 km high and three orbital configurations to answer the question. Our results show that a mountain range at the East Asian coast can amplify the impacts of orbital forcing on East Asian climate. Specifically, precipitation over the Songliao Basin in Northeastern China has significant changes as the coastal mountain range is about 4 km high. Combining our simulation results with orbitally‐controlled sedimentary deposits from the Songliao Basin, we conclude that the altitude of the coastal mountain range was very likely higher than 2 km in the Late Cretaceous.
Cortical and subcortical structural differences in psychostimulant-free ADHD youth with and without a family history of bipolar I disorder: a cross-sectional morphometric comparison
Authors: Zhu Ziyu , Lei Du , Qin Kun , Tallman Maxwell J. , Patino L. Rodrigo , Fleck David E. , Gong Qiyong , Sweeney John A. , DelBello Melissa P. , McNamara Robert K. 
Published: November 2023

AbstractAlthough attention-deficit/hyperactivity disorder (ADHD) and a family history of bipolar I disorder (BD) are associated with increased risk for developing BD, their neuroanatomical substrates remain poorly understood. This study compared cortical and subcortical gray matter morphology in psychostimulant-free ADHD youth with and without a first-degree relative with BD and typically developing healthy controls. ADHD youth (ages 10-18 years) with (‘high-risk’, HR) or without (‘low-risk’, LR) a first-degree relative with BD and healthy comparison youth (HC) were enrolled. High-resolution 3D T1-weighted images were acquired using a Philips 3.0 T MR scanner. The FreeSurfer image analysis suite was used to measure cortical thickness, surface area, and subcortical volumes. A general linear model evaluated group differences in MRI features with age and sex as covariates, and exploratory correlational analyses evaluated associations with symptom ratings. A total of n = 142 youth (mean age: 14.16 ± 2.54 years, 35.9% female) were included in the analysis (HC, n = 48; LR, n = 49; HR, n = 45). The HR group exhibited a more severe symptom profile, including higher mania and dysregulation scores, compared to the LR group. For subcortical volumes, the HR group exhibited smaller bilateral thalamic, hippocampal, and left caudate nucleus volumes compared to both LR and HC, and smaller right caudate nucleus compared with LR. No differences were found between LR and HC groups. For cortical surface area, the HR group exhibited lower parietal and temporal surface area compared with HC and LR, and lower orbitofrontal and superior frontal surface area compared to LR. The HR group exhibited lower left anterior cingulate surface area compared with HC. LR participants exhibited greater right pars opercularis surface area compared with the HC. Some cortical alterations correlated with symptom severity ratings. These findings suggest that ADHD in youth with a BD family history is associated with a more a severe symptom profile and a neuroanatomical phenotype that distinguishes it from ADHD without a BD family history.
Distinct neurexin isoforms cooperate to initiate and maintain foraging activity
Authors: Bastien Brandon L. , Cowen Mara H. , Hart Michael P. 
Published: November 2023

AbstractNeurexins are synaptic adhesion molecules that play diverse roles in synaptic development, function, maintenance, and plasticity. Neurexin genes have been associated with changes in human behavior, where variants in NRXN1 are associated with autism, schizophrenia, and Tourette syndrome. While NRXN1, NRXN2, and NRXN3 all encode major α and β isoforms, NRXN1 uniquely encodes a γ isoform, for which mechanistic roles in behavior have yet to be defined. Here, we show that both α and γ isoforms of neurexin/nrx-1 are required for the C. elegans behavioral response to food deprivation, a sustained period of hyperactivity upon food loss. We find that the γ isoform regulates initiation and the α isoform regulates maintenance of the behavioral response to food deprivation, demonstrating cooperative function of multiple nrx-1 isoforms in regulating a sustained behavior. The γ isoform alters monoamine signaling via octopamine, relies on specific expression of NRX-1 isoforms throughout the relevant circuit, and is independent of neuroligin/nlg-1, the canonical trans-synaptic partner of nrx-1. The α isoform regulates the pre-synaptic structure of the octopamine producing RIC neuron and its maintenance role is conditional on neuroligin/nlg-1. Collectively, these results demonstrate that neurexin isoforms can have separate behavioral roles and act cooperatively across neuronal circuits to modify behavior, highlighting the need to directly analyze and consider all isoforms when defining the contribution of neurexins to behavior.
Conserved temperature requirements but contrasting responses to humidity across oviposition preferences in temperate grasshoppers
Authors: Dvořák Tomáš , Knapp Michal 
Published: November 2023

AbstractThe right choice of an oviposition site is a crucial task for oviparous species without maternal care. In contrast to well investigated biotic factors, e.g., larval food preferences, parasitism, predation, and competition avoiding, abiotic factors affecting oviposition preferences in insects have been rarely investigated in comparative studies. To improve our current understanding of oviposition site selection in Orthoptera, we investigated the influence of substrate temperature and moisture on the oviposition behaviour of 14 temperate grasshopper species. Conspecific groups of adults were kept in arenas with simultaneous temperature and moisture gradients. For each ootheca produced during the experiment (n = 1192) we recorded its depth and local microclimatic conditions. Our results indicate that microclimatic oviposition preferences significantly differ among species, however, correlations between adult habitat preferences and microclimatic oviposition preferences were surprisingly weak. Even oligothermic species preferred substrate temperatures around 30 °C and some xerothermic species preferred higher humidity. The hypothesized tendency to place oothecae closer to the ground within grass tussocks under hot and dry conditions was confirmed. It is possible that species evaluate microclimatic conditions for oviposition in the context of occupied habitat, i.e., in a relative rather than absolute manner.
A deep learning model predicts the presence of diverse cancer types using circulating tumor cells
Authors: Albaradei Somayah , Alganmi Nofe , Albaradie Abdulrahman , Alharbi Eaman , Motwalli Olaa , Thafar Maha A. , Gojobori Takashi , Essack Magbubah , Gao Xin 
Published: November 2023

AbstractCirculating tumor cells (CTCs) are cancer cells that detach from the primary tumor and intravasate into the bloodstream. Thus, non-invasive liquid biopsies are being used to analyze CTC-expressed genes to identify potential cancer biomarkers. In this regard, several studies have used gene expression changes in blood to predict the presence of CTC and, consequently, cancer. However, the CTC mRNA data has not been used to develop a generic approach that indicates the presence of multiple cancer types. In this study, we developed such a generic approach. Briefly, we designed two computational workflows, one using the raw mRNA data and deep learning (DL) and the other exploiting five hub gene ranking algorithms (Degree, Maximum Neighborhood Component, Betweenness Centrality, Closeness Centrality, and Stress Centrality) with machine learning (ML). Both workflows aim to determine the top genes that best distinguish cancer types based on the CTC mRNA data. We demonstrate that our automated, robust DL framework (DNNraw) more accurately indicates the presence of multiple cancer types using the CTC gene expression data than multiple ML approaches. The DL approach achieved average precision of 0.9652, recall of 0.9640, f1-score of 0.9638 and overall accuracy of 0.9640. Furthermore, since we designed multiple approaches, we also provide a bioinformatics analysis of the gene commonly identified as top-ranked by the different methods. To our knowledge, this is the first study wherein a generic approach has been developed to predict the presence of multiple cancer types using raw CTC mRNA data, as opposed to other models that require a feature selection step.
Therapeutic effects of faricimab on aflibercept-refractory age-related macular degeneration
Authors: Tamiya Ryosuke , Hata Masayuki , Tanaka Asako , Tsuchikawa Memiri , Ueda-Arakawa Naoko , Tamura Hiroshi , Miyata Manabu , Takahashi Ayako , Kido Ai , Muraoka Yuki , Miyake Masahiro , Ooto Sotaro , Tsujikawa Akitaka 
Published: November 2023

AbstractThough vascular endothelial growth factors (VEGF) and other proangiogenic factors, such as angiopoietins (Ang), may be involved in the development of neovascular age-related macular degeneration (nvAMD), only drugs that inhibit the VEGF family are available for the treatment. The newly approved anti-VEGF drug faricimab, which also inhibits Ang-2, is expected to be effective in patients with AMD refractory to conventional anti-VEGF drugs. Therefore, we prospectively investigated the efficacy of faricimab in the treatment of aflibercept-refractory nvAMD. Patients with nvAMD who had been treated with aflibercept in the last year and required bimonthly injections were recruited. 25 eyes showed persistent exudative changes immediately before the faricimab injection (baseline). In these 25 eyes, switching to faricimab did not change visual acuity or central retinal thickness 2 months after the injection; however, 56% of eyes showed reduction or complete absorption of fluid. Notably, 25% of the eyes that showed dry macula at month 2 had no fluid recurrence for up to 4 months. These results indicate that faricimab could benefit some patients with aflibercept-refractory nvAMD.
Unravelling transcriptomic complexity in breast cancer through modulation of DARPP-32 expression and signalling pathways
Authors: Saidy Behnaz , Vasan Richa , Durant Rosie , Greener Megan-Rose , Immanuel Adelynn , Green Andrew R. , Rakha Emad , Ellis Ian , Ball Graham , Martin Stewart G. , Storr Sarah J. 
Published: November 2023

AbstractDARPP-32 is a key regulator of protein-phosphatase-1 (PP-1) and protein kinase A (PKA), with its function dependent upon its phosphorylation state. We previously identified DKK1 and GRB7 as genes with linked expression using Artificial Neural Network (ANN) analysis; here, we determine protein expression in a large cohort of early-stage breast cancer patients. Low levels of DARPP-32 Threonine-34 phosphorylation and DKK1 expression were significantly associated with poor patient prognosis, while low levels of GRB7 expression were linked to better survival outcomes. To gain insight into mechanisms underlying these associations, we analysed the transcriptome of T47D breast cancer cells following DARPP-32 knockdown. We identified 202 differentially expressed transcripts and observed that some overlapped with genes implicated in the ANN analysis, including PTK7, TRAF5, and KLK6, amongst others. Furthermore, we found that treatment of DARPP-32 knockdown cells with 17β-estradiol or PKA inhibitor fragment (6–22) amide led to the differential expression of 193 and 181 transcripts respectively. These results underscore the importance of DARPP-32, a central molecular switch, and its downstream targets, DKK1 and GRB7 in breast cancer. The discovery of common genes identified by a combined patient/cell line transcriptomic approach provides insights into the molecular mechanisms underlying differential breast cancer prognosis and highlights potential targets for therapeutic intervention.
The optical, seismic, and infrasound signature of the March 5 2022, bolide over Central Italy
Authors: Olivieri Marco , Piccinini Davide , Saccorotti Gilberto , Barghini Dario , Gardiol Daniele , Pino Nicola Alessandro , Ripepe Maurizio , Betti Giulio , Lacanna Giorgio , Arcidiaco Lorenzo 
Published: November 2023

AbstractOn March 5, 2022, a 12 kg meteoroid crossed the sky above Central Italy and was observed by three different observational systems: the PRISMA all-sky camera network (10 stations), the Italian national seismic network (61 stations), and a 4-element infrasound array. The corresponding datasets, each with its own resolution, provided three independent assessments of the trajectory, size and speed of the meteoroid. The bolide traveled across central Italy with an azimuth of 102 degrees, becoming visible at about 91 km above sea level with a velocity of about 15.4 km/s. Its visible trajectory lasted about 15 s. Reasonably, the residual portion of the ablated bolide terminated its path in the Adriatic Sea and could not be recovered. Seismic and infrasound data well match optical observations detecting the bolide Mach cone at 68 km above sea level with a back azimuth of 25 degrees with respect to the array. By comparing results from the three different systems, discrepancies are within the estimated uncertainties, thus confirming the mutual consistency of the adopted methodologies. Therefore, this study shows that different approaches can be integrated to improve the detection capability for bolide crossing the sky in monitored regions.
Optimizing the microbial synthesis of silver nanoparticles using Gloeophyllum striatum and their antimicrobial potential evaluation
Authors: Tończyk Aleksandra , Niedziałkowska Katarzyna , Lisowska Katarzyna 
Published: November 2023

AbstractThe search for new sources of silver nanoparticles (AgNPs) is highly relevant in many fields. Mycosynthesis seems to be advantageous for large-scale production, and using brown rot fungi might be a promising solution. In this study, AgNP synthesis using Gloeophyllum striatum DSM 9592 was performed under various process conditions. The resulting AgNPs were characterized using UV/Vis, FT-IR, SEM and NTA techniques and their biological activities were determined. It was found that different synthesis conditions changed the production efficiency, which was the highest in 28 s AgNPs. Moreover, temperature and shaking conditions slightly affected the activity of the resulting AgNP types. Gram-negative bacteria were generally more susceptible to the action of AgNPs with MIC values two- or three-fold lower compared to Gram-positive strains. Pseudomonas aeruginosa was the most sensitive among tested strains with a MIC value of 1.56 µg/ml. The research was additionally extended by the biofilm formation assay for this strain. It was found that AgNPs of all types led to a reduction in biofilm-forming capability of P. aeruginosa over the tested concentration range. Haemolytic and cytotoxic activity assays showed that synthesis conditions also affected AgNP toxicity. For instance, 4 ns AgNPs were the least cytotoxic and cause less than 50% reduction of fibroblast viability in the concentration that inhibits the growth of P. aeruginosa completely. These results highlight the possible utility of mycogenic silver nanoparticles as an antibacterial agent in antiseptics or other external treatments.