bluuebunny/crossref_metadata_2025_split huggingface dataset:
AI‐Driven Design System for Fabrication of Inhalable Nanocatchers for Virus Capture and Neutralization
Authors: Pang Hao‐Han , Li Nan‐Si , Hsu Ying‐Pei , Ju Shin‐Pon , Syu Guan‐Da , Du Pin‐Xian , Huang Chiung‐Yin , Wei Kuo‐Chen , Yang Hung‐Wei 
Published: November 2023

AbstractThe global pandemic presents a critical threat to humanity, with no effective rapid‐response solutions for early‐stage virus dissemination. This study aims to create an AI‐driven entry‐blocker design system (AIEB) to fabricate inhalable virus‐like nanocatchers (VLNCs) fused with entry‐blocking peptides (EBPs) to counter pandemic viruses and explore therapeutic applications. This work focuses on developing angiotensin‐converting enzyme 2 (ACE2)‐mimic domain‐fused VLNCs (ACE2@VLNCs) using AIEB and analyzing their interaction with the SARS‐CoV‐2 receptor binding domain (RBD), demonstrating their potential to hinder SARS‐CoV‐2 infection. Aerosol‐based tests show ACE2@VLNCs persist over 70 min in the air and neutralize pseudoviruses within 30 min, indicating their utility in reducing airborne virus transmission. In vivo results reveal ACE2@VLNCs mitigate over 67% of SARS‐CoV‐2 infections. Biosafety studies confirm their safety, causing no damage to eyes, skin, lungs, or trachea, and not eliciting significant immune responses. These findings offer crucial insights into pandemic virus prevention and treatment, highlighting the potential of the ACE2@VLNCs system as a promising strategy against future pandemics.
Self‐Assembled Multiepitope Nanovaccine Provides Long‐Lasting Cross‐Protection against Influenza Virus
Authors: Nie Jiaojiao , Wang Qingyu , Li Chenxi , Zhou Yongfei , Yao Xin , Xu Lipeng , Chang Yaotian , Ding Fan , Sun Lulu , Zhan Li , Zhu Lvzhou , Xie Kunpeng , Wang Xu , Shi Yuhua , Zhao Qi , Shan Yaming 
Published: November 2023

AbstractSeasonal influenza vaccines typically provide strain‐specific protection and are reformulated annually, which is a complex and time‐consuming process. Multiepitope vaccines, combining multiple conserved antigenic epitopes from a pathogen, can trigger more robust, diverse, and effective immune responses, providing a potential solution. However, their practical application is hindered by low immunogenicity and short‐term effectiveness. In this study, multiple linear epitopes from the conserved stem domain of hemagglutinin and the ectodomain of matrix protein 2 are combined with the Helicobacter pylori ferritin, a stable self‐assembled nanoplatform, to develop an influenza multiepitope nanovaccine, named MHF. MHF is prokaryotically expressed in a soluble form and self‐assembles into uniform nanoparticles. The subcutaneous immunization of mice with adjuvanted MHF induces cross‐reactive neutralizing antibodies, antibody‐dependent cell‐mediated cytotoxicity, and cellular immunity, offering complete protection against H3N2 as well as partial protection against H1N1. Importantly, the vaccine cargo delivered by ferritin triggers epitope‐specific memory B‐cell responses, with antibody level persisting for over 6 months post‐immunization. These findings indicate that self‐assembled multiepitope nanovaccines elicit potent and long‐lasting immune responses while significantly reducing the risk of vaccine escape mutants, and offer greater practicality in terms of scalable manufacturing and genetic manipulability, presenting a promising and effective strategy for future vaccine development.
Hybrid Endometrial‐Derived Hydrogels: Human Organoid Culture Models and In Vivo Perspectives
Authors: Gómez‐Álvarez María , Bueno‐Fernandez Clara , Rodríguez‐Eguren Adolfo , Francés‐Herrero Emilio , Agustina‐Hernández Marcos , Faus Amparo , Gisbert Roca Fernando , Martínez‐Ramos Cristina , Galán Amparo , Pellicer Antonio , Ferrero Hortensia , Cervelló Irene 
Published: November 2023

AbstractThe endometrium plays a vital role in fertility, providing a receptive environment for embryo implantation and development. Understanding the endometrial physiology is essential for developing new strategies to improve reproductive healthcare. Human endometrial organoids (hEOs) are emerging as powerful models for translational research and personalized medicine. However, most hEOs are cultured in a 3D microenvironment that significantly differs from the human endometrium, limiting their applicability in bioengineering. This study presents a hybrid endometrial‐derived hydrogel that combines the rigidity of PuraMatrix (PM) with the natural scaffold components and interactions of a porcine decellularized endometrial extracellular matrix (EndoECM) hydrogel. This hydrogel provides outstanding support for hEO culture, enhances hEO differentiation efficiency due to its biochemical similarity with the native tissue, exhibits superior in vivo stability, and demonstrates xenogeneic biocompatibility in mice over a 2‐week period. Taken together, these attributes position this hybrid endometrial‐derived hydrogel as a promising biomaterial for regenerative treatments in reproductive medicine.
Piezoionic High Performance Hydrogel Generator and Active Protein Absorber via Microscopic Porosity and Phase Blending
Authors: Lu Xi , Chen Yao , Zhang Yihe , Cheng Jiajun , Teng Kaixuan , Chen Yunfan , Shi Jing , Wang Danlei , Wang Long , You Shaohua , Feng Zeguo , An Qi 
Published: November 2023

AbstractGenerating electricity in hydrogel is very important but remains difficult. Hydrogel with electricity generation capability is more capable in bio‐relevant tasks such as tissue engineering, artificial skin, or medical treatment, because electricity is indispensable in regulating physiological activities. Here, a porous and phase blending hydrogel structure for effective piezoionic electricity generation is developed. Dynamic electric field is generated taking advantage of the difference in streaming speeds of sodium and chloride in the material. Microscopic porosity and hydrophilic‐hydrophobic phase blending are the two key factors for prominent piezoionic performance. Voltages as high as 600 mV are first realized in hydrogels in response to medical ultrasound stimulation. The hydrogel structure is also subjective to effective substance exchange and can actively enrich proteins from surroundings under mechanical stimuli. Preliminary applications in neural stimulation, constructing complex spatial‐temporal chemical and electric field distribution patterns, mimetic tactile sensor, sample pretreatment in fast detection, and enzyme immobilization are demonstrated.
Room Temperature NUV‐To‐NIR Up‐ and Down‐Conversion Photoluminescence in Erbium‐Doped GaAs
Authors: Cheng Yuanbo , Zuo Zongyan , Yao Jinshan , Zhang Kedong , Lu Yang , Li Chen , Deng Yu , Zhang Xuejin , Lu Hong , Chen Yan‐Feng 
Published: November 2023

AbstractErbium (Er)‐doped III‐V semiconductors are promising for optoelectronic devices due to the unique intra‐4f electronic transitions of Er3+; however, the Er‐related luminescence at room temperature has always been challenging to obtain. In this work, High crystalline quality ErAs:GaAs films are grown with self‐assembled ErAs nanoparticles embedded within the GaAs matrix by molecular beam epitaxy. Rich photoluminescence spectra in a broad wavelength ranging from near‐ultraviolet to near‐infrared are observed at room temperature and confirmed to be Er‐related. A carrier‐mediated energy transfer up‐conversion mechanism is proposed, and the pump power‐dependent photoluminescence results reveal that the excited‐state absorption is governed by a single photonic process. The efficient interaction and energy transfer between the semiconductor matrix and Er3+ ions offered by this unique nanocomposite material provide a promising route to achieve novel optoelectronic devices with the potential to cover a broad wavelength range.
Optimized Two‐Color Single‐Molecule Tracking of Fast‐Diffusing Membrane Receptors
Authors: Schirripa Spagnolo Chiara , Moscardini Aldo , Amodeo Rosy , Beltram Fabio , Luin Stefano 
Published: November 2023

AbstractSingle particle tracking (SPT) combined with total internal reflection fluorescence microscopy (TIRFm) is an outstanding approach to decipher mechanisms on the cell membrane at the nanoscale. Multicolor configurations, needed to investigate interactions, are still hindered by several challenges. This work systematically and quantitatively analyzes the impact of necessary elements of SPT‐TIRFm setups on the signal‐to‐noise ratio (SNR), which must be optimized especially in dynamic studies needing minimally invasive dyes for biomolecule labeling. Autofluorescence originating from commonly used optical glass results in the dominant limiting factor in TIRFm, and a cover glass material is tested yielding significant SNR improvements in multichannel TIRFm. Moreover, methodologies are optimized for reducing fluorophore photobleaching in multicolor implementations requiring simultaneous stabilization of multiple dyes. The developed strategies are applied to the fast p75NTR receptors labeled by two fluorophores on the membrane of living cells, achieving reliable, simultaneous two‐color SPT, contrary to configurations using standard cover glasses. This work highlights the importance of optical materials suitable for microscopy and with reduced autofluorescence for increasing sensitivity toward ultimate spatiotemporal resolutions. In particular, the present protocols can pave the way for multicolor super‐resolved localization and tracking of single molecules by TIRFm, greatly expanding the potential of SPT.
Unveiling The Role of Cu+ Doping in Rb2AgBr3 Scintillators toward Enhanced Photoluminescence Quantum Efficiency and Light Yield
Authors: Hu Yakun , Jin Jiance , Han Kai , Xia Zhiguo 
Published: November 2023

AbstractDoping in luminescent metal halides has become an effective strategy for tailoring the optoelectronic properties. Herein, Cu+ is introduced into Rb2AgBr3 to design the synthesis of Rb2AgBr3:xCu+, which possess cold‐white light emission at room‐temperature without changing the spectral profiles; however, the Cu+ incorporation significantly enhances the photoluminescence quantum efficiency to 98.8% in Rb2AgBr3:0.05Cu+. Additionally, a high scintillation light yield of up to 79 250 photons MeV−1 and a low detection limit of 714.83 nGy s−1 have been achieved via this Cu+ doping engineering. X‐ray imaging with a spatial resolution of up to 5.8 lp mm−1 is further realized using a large scintillator film prepared by mixing polydimethylsiloxane with Cu+ doped Rb2AgBr3 scintillators. This study provides a Cu+ doping design principle for improving both the photoluminescence and scintillation performances of metal halides.
Enantioselective Syntheses of Multiply Functionalized Six‐Membered Carbocycles from Nitroalkenes and γ,δ‐Unsaturated ß‐Ketoesters using Chiral Catalysts Derived from Cobalt(III) and 1,2‐Diphenylethylenediamine
Authors: Wang Katherine , Bhuvanesh Nattamai , Gladysz John A. 
Published: November 2023

AbstractCondensations of the title educts R4CH=CH(C=O)CH2CO2Et and R6CH=CHNO2 in the presence of NEt3 and the catalyst Λ‐[Co((S,S)‐dpen)3]3+2Cl−B(C6F5)4− (both 10 mol%, 0 °C; dpen=1,2‐diphenylethylenediamine) afford R6CHCH(NO2)CH(R4)CH2C(OH)CCO2Et, with three contiguous carbon stereocenters, as >99:1 to 85:15 mixtures of diastereomers (ten examples). The major diastereomers are isolated in 81–63% yields and 97–76% ee. When R4 and R6 are both aryl groups, additions of 1,1,3,3‐tetramethylquanidine/CH3CN epimerize the CHNO2 moieties, affording 78:22 to 67:33 mixtures of the new/old diastereomers (2 h, RT). Alternatively, epimerization to >99:1 mixtures occurs spontaneously over 4 weeks in hexanes/isopropanol. These transformations entail two consecutive Michael additions, and two crystal structures confirm the relative and absolute product configurations. These assignments are supported by NMR data in solution and literature studies of similar compounds. Overall, catalyst Λ‐[Co((S,S)‐dpen)3]3+2Cl−B(C6F5)4− affords superior yields and enantio‐ or diastereoselectivities compared to those previously reported for the title reaction.
Electrochemical Oxidation of Indoles to Access Tryptanthrins at Room Temperature
Authors: Guo Ziyi , Liu Yi , Li Hang , Wu Jiaping , Xie Meihua , Zhang Jitan 
Published: November 2023

AbstractAn electrooxidative reconstruction of the indole core under ambient conditions is reported, resulting in the construction of tryptanthrins. This electrochemical strategy simplifies molecular skeleton‐hopping by employing commercially available NH‐indole feedstock as the starting materials. Furthermore, the synthetic value of this skeletal reconstruction was demonstrated by divergent further transformations. A preliminary mechanistic investigation involving control experiments and cyclovoltammetric studies showed the indispensability of electricity, as well as the importance of oxygen and TEMPO, thereby revealing a possible reaction pathway.
Nanotechnology and Cancer Bioelectricity: Bridging the Gap Between Biology and Translational Medicine
Authors: Moreddu Rosalia 
Published: November 2023

AbstractBioelectricity is the electrical activity that occurs within living cells and tissues. This activity is critical for regulating homeostatic cellular function and communication, and disruptions of the same can lead to a variety of conditions, including cancer. Cancer cells are known to exhibit abnormal electrical properties compared to their healthy counterparts, and this has driven researchers to investigate the potential of harnessing bioelectricity as a tool in cancer diagnosis, prognosis, and treatment. In parallel, bioelectricity represents one of the means to gain fundamental insights on how electrical signals and charges play a role in cancer insurgence, growth, and progression. This review provides a comprehensive analysis of the literature in this field, addressing the fundamentals of bioelectricity in single cancer cells, cancer cell cohorts, and cancerous tissues. The emerging role of bioelectricity in cancer proliferation and metastasis is introduced. Based on the acknowledgement that this biological information is still hard to access due to the existing gap between biological findings and translational medicine, the latest advancements in the field of nanotechnologies for cellular electrophysiology are examined, as well as the most recent developments in micro‐ and nano‐devices for cancer diagnostics and therapy targeting bioelectricity.
IGF2BP2 Drives Cell Cycle Progression in Triple‐Negative Breast Cancer by Recruiting EIF4A1 to Promote the m6A‐Modified CDK6 Translation Initiation Process
Authors: Xia Tian , Dai Xin‐Yuan , Sang Ming‐Yi , Zhang Xu , Xu Feng , Wu Jing , Shi Liang , Wei Ji‐Fu , Ding Qiang 
Published: November 2023

AbstractIGF2BP2 is a new identified N6‐methyladenosine (m6A) reader and associated with poor prognosis in many tumors. However, its role and related mechanism in breast cancer, especially in triple‐negative breast cancer (TNBC), remains unclear. In this study, it is found that IGF2BP2 is highly expressed in TNBC due to the lower methylation level in its promoter region. Functional and mechanical studies displayed that IGF2BP2 could promote TNBC proliferation and the G1/S phase transition of the cell cycle via directly regulating CDK6 in an m6A‐dependent manner. Surprising, the regulation of protein levels of CDK6 by IGF2BP2 is related to the changes in translation rate during translation initiation, rather than mRNA stability. Moreover, EIF4A1 is found to be recruited by IGF2BP2 to promote the translation output of CDK6, providing new evidence for a regulatory role of IGF2BP2 between m6A methylation and translation. Downregulation of IGF2BP2 in TNBC cell could enhance the sensitivity to abemaciclib, a CDK4/6 inhibitor. To sum up, our study revealed IGF2BP2 could facilitate the translation output of CDK6 via recruiting EIF4A1 and also provided a potential therapeutic target for the diagnosis and treatment of TNBC, as well as a new strategy for broadening the drug indications for CDK4/6 inhibitors.
A Novel Bioswitchable miRNA Mimic Delivery System: Therapeutic Strategies Upgraded from Tetrahedral Framework Nucleic Acid System for Fibrotic Disease Treatment and Pyroptosis Pathway Inhibition
Authors: Jiang Yueying , Li Songhang , Shi Ruijianghan , Yin Wumeng , Lv Weitong , Tian Taoran , Lin Yunfeng 
Published: November 2023

AbstractThere has been considerable interest in gene vectors and their role in regulating cellular activities and treating diseases since the advent of nucleic acid drugs. MicroRNA (miR) therapeutic strategies are research hotspots as they regulate gene expression post‐transcriptionally and treat a range of diseases. An original tetrahedral framework nucleic acid (tFNA) analog, a bioswitchable miR inhibitor delivery system (BiRDS) carrying miR inhibitors, is previously established; however, it remains unknown whether BiRDS can be equipped with miR mimics. Taking advantage of the transport capacity of tetrahedral framework nucleic acid (tFNA) and upgrading it further, the treatment outcomes of a traditional tFNA and BiRDS at different concentrations on TGF‐β‐ and bleomycin‐induced fibrosis simultaneously in vitro and in vivo are compared. An upgraded traditional tFNA is designed by successfully synthesizing a novel BiRDS, carrying a miR mimic, miR‐27a, for treating skin fibrosis and inhibiting the pyroptosis pathway, which exhibits stability and biocompatibility. BiRDS has three times higher efficiency in delivering miRNAs than the conventional tFNA with sticky ends. Moreover, BiRDS is more potent against fibrosis and pyroptosis‐related diseases than tFNAs. These findings indicate that the BiRDS can be applied as a drug delivery system for disease treatment.
Loss of ESRP2 Activates TAK1‐MAPK Signaling through the Fetal RNA‐Splicing Program to Promote Hepatocellular Carcinoma Progression
Authors: Yan Qian , Fang Xiaona , Liu Xiaoxia , Guo Sai , Chen Siqi , Luo Min , Lan Ping , Guan Xin‐Yuan 
Published: November 2023

AbstractTumors usually display fetal‐like characteristics, and many oncofetal proteins have been identified. However, fetal‐like reprogramming of RNA splicing in hepatocellular carcinoma (HCC) is poorly understood. Here, it is demonstrated that the expression of epithelial splicing regulatory protein 2 (ESRP2), an RNA splicing factor, is suppressed in fetal hepatocytes and HCC, in parallel with tumor progression. By combining RNA‐Seq with splicing analysis, it is identified that ESRP2 controls the fetal‐to‐adult switch of multiple splice isoforms in HCC. Functionally, ESRP2 suppressed cell proliferation and migration by specifically switching the alternative splicing (AS) of the TAK1 gene and restraining the expression of the fetal and oncogenic isoform, TAK1_ΔE12. Notably, aberrant TAK1 splicing led to the activation of p38MAPK signaling and predicted poor prognosis in HCC patients. Further investigation revealed that TAK1_ΔE12 protein interacted closely with TAB3 and formed liquid condensation in HCC cells, resulting in p38MAPK activation, enhanced cell migration, and accelerated tumorigenesis. Loss of ESRP2 sensitized HCC cells to TAK1 kinase inhibitor (TAK1i), promoting pyroptotic cell death and CD8+ T cell infiltration. Combining TAK1i with immune checkpoint therapy achieved potent tumor regression in mice. Overall, the findings reveal a previously unexplored onco‐fetal reprogramming of RNA splicing and provide novel therapeutic avenues for HCC.
A Facile Design for Water‐Oxidation Molecular Catalysts Precise Assembling on Photoanodes
Authors: Jiang Wenchao , Li Siyuan , Sui Qi , Gao Yujie , Li Fei , Xia Lixin , Jiang Yi 
Published: November 2023

AbstractRegulating the interfacial charge transfer behavior between cocatalysts and semiconductors remains a critical challenge for attaining efficient photoelectrochemical water oxidation reactions. Herein, using bismuth vanadate (BiVO4) photoanode as a model, it introduces an Au binding bridge as holes transfer channels onto the surfaces of BiVO4, and the cyano‐functionalized cobalt cubane (Co4O4) molecules are preferentially immobilized on the Au bridge due to the strong adsorption of cyano groups with Au nanoparticles. This orchestrated arrangement facilitates the seamless transfer of photogenerated holes from BiVO4 to Co4O4 molecules, forming an orderly charge transfer pathway connecting the light‐absorbing layer to reactive sites. An exciting photocurrent density of 5.06 mA cm−2 at 1.23 V versus the reversible hydrogen electrode (3.4 times that of BiVO4) is obtained by the Co4O4@Au(A)/BiVO4 photoanode, where the surface charge recombination is almost completely suppressed accompanied by a surface charge transfer efficiency over 95%. This work represents a promising strategy for accelerating interfacial charge transfer and achieving efficient photoelectrochemical water oxidation reaction.
Poly(2‐vinylpyridine) as an Additive for Enhancing N‐Type Organic Thin‐Film Transistor Stability
Authors: Brixi Samantha , Dindault Chloé , King Benjamin , Lamontagne Halynne R. , Shuhendler Adam J. , Swaraj Sufal , Lessard Benoît H. 
Published: November 2023

AbstractN‐type organic semiconductors are particularly susceptible to degradation by ambient air. One such solution to this issue is to include additives in the inks these semiconductors would be cast from that would enhance device stability after film deposition. This method would reduce the number of processing steps needed to fabricate devices compared to other stabilization methods, such as encapsulation. In this study, the stabilization of n‐type performance of the semiconductor poly{[N,N′‐bis(2‐octyldodecyl)‐naphthalene‐1,4,5,8‐bis(dicarboximide)‐2,6‐diyl]‐alt‐5,5′‐(2,29‐bisthiophene)} (P(NDI2OD‐T2)) when it is blended with an increasing proportion by weight of poly(2‐vinylpyridine) (P2VP) is reported. The simple synthesis of P2VP also makes it an ideal candidate material for large‐scale applications. Concentrations as low as 0.1% P2VP incorporated into the P(NDI2OD‐T2) blends provided an immediate stabilization effect, and at 10% and 50%, longer‐term stability after one week is observed.