bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Anti‐Inflammatory Medications in Managing Atypical Gingivitis Associated With Hypermobile Ehlers‐Danlos Syndrome: A Case Report
Authors: Khocht Ahmed , Calem Ben , Deasy Michael 
Published: February 2005

Background: Ehlers‐Danlos syndrome (EDS) is a group of collagen disorders primarily affecting the skin and joints. This case report describes the periodontal management of a case of hypermobile EDS (type III) associated with atypical gingivitis.Methods: A 17‐year‐old Caucasian female with a history of EDS type III presented with erythematous mucogingival lesions. An incisional biopsy was taken. The treatment objective was to control the mucogingival inflammatory lesions. Plaque control measures were followed with anti‐inflammatory medications. Prednisone was administered systemically for 3 weeks (first week: 30 mg/day, second week: 15 mg/day, and third week: 5 mg/day), and was followed with topical applications of clobetasol (0.05%) twice daily for 2 weeks. Persistent marginal gingival inflammation was managed with a 3‐month course of doxycycline (20 mg) twice/day. The patient was placed on a 3‐month maintenance program and monitored for over a year.Results: Histopathology showed multiple granulomas enclosing fragments of crystalline foreign material. Steriodal anti‐inflammatory medications partially reduced the size and intensity of the mucogingival lesions. Doxycycline controlled the residual marginal inflammation. A 3‐month maintenance interval helped in maintaining the results obtained.Conclusions: The collagen disorder may have facilitated the introduction and lodging of foreign material within the gingival tissues, initiating a foreign body reaction. Poor healing associated with EDS excluded surgical excision and necessitated the use of anti‐inflammatory medications. A combined approach of plaque control measures along with anti‐inflammatory medications was helpful in controlling the mucogingival inflammatory lesions associated with this condition. J Periodontol 2004;75:1547‐1552.
Cover Picture: Periodical Micro‐Structuring of Hydride Containing Metastable Aluminumoxide using Laser Interference Metallurgy (Adv. Eng. Mater. 1‐2/2005)
Authors: Veith M. , Andres K. , Petersen C. , Daniel C. , Holzapfel C. , Mücklich F. 
Published: February 2005

AbstractThe cover shows a light microscopic image of a patterned HAlO film with Al‐Al2O3 lines (dark regions). The micro‐patterning is obtained by laser interference metallurgy, and more about periodical microstructuring using laser interference metallurgy can be found in the article by Veith, Mücklich et al. on page 27.
Self‐Cleaning Fabrics for Decontamination of Organophosphorous Pesticides and Related Chemical Agents (Adv. Mater. 2004, 23‐24, 2112)
Authors: Singh A. , Lee Y. , Dressick W. J. 
Published: February 2005

AbstractTo view the original paper use http://dx.doi.org/10.1002/adma.200400660.
Association between BDNF val66 met genotype and episodic memory
Authors: Dempster Emma , Toulopoulou Timothea , McDonald Colm , Bramon Elvira , Walshe Muriel , Filbey Francesca , Wickham Harvey , Sham Pak C. , Murray Robin M. , Collier David A. 
Published: February 2005

AbstractThe val66 met polymorphism of brain derived neurotrophic factor (BDNF) has been associated with variability in episodic memory [Egan et al., 2003]. In an attempt to replicate this finding, we genotyped 206 individuals (92 affected with schizophrenia or a related disorder and 114 unaffected relatives) from the Maudsley Family Study for the BDNF val66 met polymorphism. We analyzed the effect of this polymorphism on episodic memory using the Wechsler Memory Scale, revised version (WMS‐R) by regression analysis between the WMS delayed score of logical memory and genotype (corrected for age, sex, and IQ). We found the met66 allele conferred a lower score on the WMS delayed measure (R2 = 0.014 P = 0.09), which was not significant. When cases and unaffected relatives were analyzed separately, met66 was associated with a lower score on the WMS delayed measure in the relatives only (R2 = 0.077 P = 0.01), which is consistent with previous findings. © 2005 Wiley‐Liss, Inc.
Heterotrophic high cell‐density fed‐batch cultures of the phycocyanin‐producing red alga Galdieria sulphuraria
Authors: Schmidt Rikke Ankerstjerne , Wiebe Marilyn G. , Eriksen Niels Thomas 
Published: February 2005

AbstractGrowth and phycocyanin production in batch and fed‐batch cultures of the microalga Galdieria sulphuraria 074G, which was grown heterotrophically in darkness on glucose, fructose, sucrose, and sugar beet molasses, was investigated. In batch cultures, specific growth rates and yields of biomass dry weight on the pure sugars were 1.08–1.15 day−1 and 0.48–0.50 g g−1, respectively. They were slightly higher when molasses was the carbon source. Cellular phycocyanin contents during the exponential growth phase were 3–4 mg g−1 in dry weight. G. sulphuraria was able to tolerate concentrations of glucose and fructose of up to 166 g L−1 (0.9 M) and an ammonium sulfate concentration of 22 g L−1 (0.17 M) without negative effects on the specific growth rate. When the total concentration of dissolved substances in the growth medium exceeded 1–2 M, growth was completely inhibited. In carbon‐limited fed‐batch cultures, biomass dry weight concentrations of 80–120 g L−1 were obtained while phycocyanin accumulated to concentrations between 250 and 400 mg L−1. These results demonstrate that G. sulphuraria is well suited for growth in heterotrophic cultures at very high cell densities, and that such cultures produce significant amounts of phycocyanin. Furthermore, the productivity of phycocyanin in the heterotrophic fed‐batch cultures of G. sulphuraria was higher than is attained in outdoor cultures of Spirulina platensis, where phycocyanin is presently obtained. © 2005 Wiley Periodicals, Inc.
Ring‐Closing Metathesis of C‐Terminal Allylglycine Residues with an N‐Terminal β‐Vinyl‐Substituted Phosphotyrosyl Mimetic as an Approach to Novel Grb2 SH2 Domain‐Binding Macrocycles
Authors: Oishi Shinya , Shi Zhen‐Dan , Worthy Karen M. , Bindu Lakshman K. , Fisher Robert J. , Burke Terrence R. 
Published: February 2005

AbstractRing‐closing metathesis (RCM) of peptides often requires insertion of allylglycines at the intended sites of ring juncture, which can result in the displacement of residues that are needed for biological activity. This type of side‐chain deletion can be avoided by appending β‐vinyl substituents onto the parent residues at the intended sites of ring juncture, thereby effectively converting them into functionalized allylglycine equivalents. Such an approach has been previously applied in modified form to growth‐factor receptor bound 2 (Grb2) SH2 domain‐binding peptides by using an N‐terminal β‐vinyl‐functionalized phosphotyrosyl mimetic and C‐terminal 2‐allyl‐3‐aryl‐1‐propanamides that lacked the α‐carboxyl portion of allylglycine residues. These C‐terminal moieties involved lengthy synthesis and once prepared, required an individual total synthesis of each final macrocycle. Work reported herein significantly enhances the versatility of the original approach through the use of C‐terminal allylglycine amides that can be prepared from commercially available L‐ and D‐allylglycines and suitable amines. This methodology could be generally useful where macrocylization is desired with maintenance of functionality at a site of ring juncture.
Synthesis and Structural Metastability of CdTe Nanowires
Authors: Kumar Sandeep , Ade Martin , Nann Thomas 
Published: February 2005

AbstractA new organometallic preparation method is described for CdTe nanowires with a high aspect ratio and a predominantly metastable wurtzite phase. The optical and morphological properties of the resulting nanowires were studied, as well as the influence of elevated temperatures on the crystallographic properties. A phase transition from wurtzite to sphalerite was observed at about 500 °C. The results show that the wurtzite phase is stabilized by the synthetic method and the surfactants.
Direct effects of T‐bet and MHC class I expression, but not STAT1, on peripheral NK cell maturation
Authors: Robbins Scott H. , Tessmer Marlowe S. , Van Kaer Luc , Brossay Laurent 
Published: February 2005

AbstractThe homeostatic maturation of NK cells is severely impaired in mice lacking the transcription factor T‐bet, and the expression of the NK cell maturation marker killer cell lectin‐like receptor G1 (KLRG1) has been shown to be dependent on MHC class I molecules. Interferon (IFN)‐γ signaling via the signal transducer and activator of transcription (STAT)1 is vital for T‐bet and MHC class I induction. Here we investigated the relationship between STAT1, T‐bet, and MHC class I molecules with regard to the phenotypic maturation of peripheral NK cells. We demonstrate that, to varying degrees, the maturation status of peripheral NK cells is impaired in naive mice with deficiencies in STAT1, T‐bet, or MHC class I molecules. We find that in naive animals, the expression of wild‐type levels of MHC class I molecules in trans is sufficient to restore the maturation profiles of STAT1–/– NK cells in vivo. In contrast, expression of T‐bet is required in cis for normal NK cell maturation to occur. Additionally, we demonstrate that the activation‐induced maturation of NK cells during the course of murine cytomegalovirus (MCMV) infection does not require expression of MHC class I molecules or STAT1 but is severely delayed in the absence of T‐bet.
Hippocampal 72‐kDa heat shock protein expression varies according to mice learning performance independently from chronic exposure to stress
Authors: Ambrosini Maria Vittoria , Mariucci Giuseppina , Tantucci Michela , Van Hooijdonk Lenneke , Ammassari‐Teule Martine 
Published: February 2005

AbstractThe possibility that the inducible 72‐kDa heat shock protein (hsp72) is involved in learning‐related plasticity mechanisms was investigated in two inbred strains of mice that show spontaneous differences in spatial learning performance as well as an opposite reactivity to stress. Induction of hsp72 after radial maze training was measured by immunoblotting in the hippocampus of C57BL/6 (C57) and DBA/2 (DBA) inbred mice exposed or nonexposed to chronic acoustic stress. In agreement with previous studies, inter‐strain differences in radial maze performance were found in nonstressed mice with C57 mice showing the higher scores. Chronic acoustic stress, however, impaired performance in the high‐learner C57 strain and improved performance in the low‐learner DBA strain. Western blot analysis revealed that post‐training expression of hsp72 was low in the condition each strain was showing the higher‐performance (nonstressed C57 and stressed DBA) and high in the condition each strain was showing the lower performance (stressed C57 and nonstressed DBA). These findings indicate that expression of hsp72 in the hippocampus varies as a function of the learning performance independently from exposure to chronic acoustic stress. © 2005 Wiley‐Liss, Inc.
Induction of cytotoxicity in human lung adenocarcinoma cells by 6‐O‐carboxypropyl‐α‐tocotrienol, a redox‐silent derivative of α‐tocotrienol
Authors: Yano Yoshihisa , Satoh Haruna , Fukumoto Keiko , Kumadaki Itsumaro , Ichikawa Tomio , Yamada Kazuhiko , Hagiwara Kiyokazu , Yano Tomohiro 
Published: February 2005

AbstractTocotrienols are one of the most potent anticancer agents of all natural compounds and the anticancer property may be related to the inactivation of Ras family molecules. The anticancer potential of tocotrienols, however, is weakened due to its short elimination half life in vivo. To overcome the disadvantage and reinforce the anticancer activity in tocotrienols, we synthesized a redox‐silent analogue of α‐tocotrienol (T3), 6‐O‐carboxypropyl‐α‐tocotrienol (T3E). We estimated the possibility of T3E as a new anticancer agent against lung adenocarcinoma showing poor prognosis based on the mutation of ras gene. T3E showed cytotoxicity against A549 cells, a human lung adenocarcinoma cell line with a ras gene mutation, in a dose‐dependent manner (0–40 μM), whereas T3 and a redox‐silent analogue of α‐tocopherol (T), 6‐O‐carboxypropyl‐α‐tocopherol (TE), showed much less cytotoxicity in cells within 40 μM. T3E cytotoxicity was based on the accumulation of cells in the G1‐phase of the cell‐cycle and the subsequent induction of apoptosis. Similar to this event, 24‐hr treatment of A549 cells with 40 μM T3E caused the inhibition of Ras farnesylation, and a marked decrease in the levels of cyclin D required for G1/S progression in the cell‐cycle and Bcl‐xL, a key anti‐apoptotic molecule. Moreover, the T3E‐dependent inhibition of RhoA geranyl‐geranylation is an inducing factor for the occurrence of apoptosis in A549 cells. Our results suggest that T3E suppresses Ras and RhoA prenylation, leading to negative growth control against A549 cells. In conclusion, a redox‐silent analogue of T3, T3E may be a new candidate as an anticancer agent against lung adenocarcinoma showing poor prognosis based on the mutation of ras genes. © 2005 Wiley‐Liss, Inc.
Neuroserpin (PI‐12) is upregulated in high‐grade prostate cancer and is associated with survival
Authors: Hasumi Hisashi , Ishiguro Hitoshi , Nakamura Masashi , Sugiura Shinpei , Osada Yutaka , Miyoshi Yasuhide , Fujinami Kiyoshi , Yao Masahiro , Hamada Kenji , Yamada‐Okabe Hisafumi , Kubota Yoshinobu , Uemura Hiroji 
Published: February 2005

AbstractWe carried out Genechip analysis using prostate cancer and non‐malignant tissue to identify specific genes related to prostate cancer. We focused on neuroserpin (PI‐12), which has been identified as one of the genes with high expression in prostate cancer. We analyzed the relationship between its expression pattern and clinical characteristics. Prostate cancer and normal prostate tissue were analyzed by Affymetrix GeneChip technology. We carried out real‐time quantitative PCR on a total of 102 specimens: 45 of normal prostate, 45 of previously untreated prostate cancer (constituting 45 pairs of samples obtained at radical prostatectomy, with each pair dissected from the same prostate specimen) and 12 of recurrent hormone refractory prostate cancer (HRPC). Results showed that the neuroserpin gene was more highly expressed in prostate cancer than in normal prostate tissue. Neuroserpin expression in untreated prostate cancer was significantly higher than that in normal prostate. In HRPC it was significantly higher than that in untreated prostate cancer and normal prostate. In untreated prostate cancer, neuroserpin expression was significantly higher in high grade tumors such as poorly differentiated adenocarcinoma than in lower grade tumors such as well or moderately differentiated adenocarcinoma. Higher neuroserpin expression was associated with shorter recurrence‐free survival after radical prostatectomy, shorter recurrence‐free survival in HRPC patients and shorter overall survival in HRPC patients. The neuroserpin gene may be associated with the development, progression and aggressiveness of prostate cancer. Our present data suggests that higher neuroserpin expression may predict an unfavorable outcome after radical prostatectomy or hormone therapy. © 2005 Wiley‐Liss, Inc.
Lithium‐mediated downregulation of PKB/Akt and cyclin E with growth inhibition in hepatocellular carcinoma cells
Authors: Erdal Esra , Ozturk Nuri , Cagatay Tolga , Eksioglu‐Demiralp Emel , Ozturk Mehmet 
Published: February 2005

AbstractWe studied in vitro effects of glycogen synthase kinase 3β (GSK3β)‐inhibitor lithium on the growth of hepatocellular carcinoma (HCC) cells. Lithium induced strong growth inhibition (>70%) in 75% (n = 9 of 12) of cell lines, apparently independent from the status of major genes that are mutated in HCC including p53, p16INK4a, β‐catenin and Axin1. Comparative studies with a growth‐sensitive Huh7 and growth‐resistant Hep40 cell lines showed that lithium induces growth arrest in Huh7 cells but not in Hep40 cells. Lithium induced the accumulation of N‐terminally phosphorylated inactive form of GSK3β with concomitant increase in β‐catenin and β‐catenin/TCF transcriptional activity in both cell lines. This suggests that lithium‐mediated HCC growth inhibition is independent of its well‐known stimulatory effect on Wnt‐β‐catenin signaling. The main differences between Huh7 and Hep40 responses to lithium treatment were observed at the levels PKB/Akt and cyclin E proteins. Lithium induced depletion of both proteins in growth‐sensitive Huh7, but not in growth‐resistant Hep40 cells. PKB/Akt and Cyclin E are 2 major proteins that are known to be constitutively active in HCC. The targeting of both proteins with lithium may be the main reason why most HCC cells are responsive to lithium‐mediated growth inhibition, independent of their p53, retinoblastoma and Wnt‐β‐catenin pathways. The exploration of molecular mechanisms involved in lithium‐mediated growth inhibition in relation with PKB/Akt and cyclin E downregulation may provide new insights for therapy of liver tumors. © 2005 Wiley‐Liss, Inc.
Yeast (ADPribosyl)ation: Revisiting a controversial question
Authors: Faraone‐Mennella Maria Rosaria , De Maio Anna , Petrella Anna , Syntichaki Evangelia , Kerbalaeva Albina M. , Nasmetova S.M. , Goulyamova Toshkon G. , Farina Benedetta 
Published: February 2005

AbstractThe controversy about the occurrence of an (ADPribosyl)ating activity in yeast is still standing up. Here we discuss this topic on the basis of results obtained with classic experiments proposed over years as basis to characterize an (ADPribosyl)ation system in any organism. Independent results obtained in two different laboratories were in line with each other and went towards the occurrence of an active (ADPribosyl)ating system in Saccharomyces cerevisiae. In fact data collected from nuclear preparations of cultured cells matched those from baker's yeast and lyophilized yeast cells. Yeast (ADPribosyl)ating enzyme is a protein of 80–90 kDa, as determined by electrophoresis on polyacrylamide gel in sodium dodecyl sulphate, followed by immunoblotting with antibodies against anti‐poly(ADPribose) polymerase catalytic site. It synthesizes products, that, after digestion with phosphodiesterase, co‐migrates mainly with phosphoribosyl adenosine monophosphate after thin layer chromatography on silica gel plate. © 2005 Wiley‐Liss, Inc.
Comparison of potential protective effects of melatonin, indole‐3‐propionic acid, and propylthiouracil against lipid peroxidation caused by potassium bromate in the thyroid gland
Authors: Karbownik Malgorzata , Stasiak Magdalena , Zasada Krzysztof , Zygmunt Arkadiusz , Lewinski Andrzej 
Published: February 2005

AbstractPotassium bromate (KBrO3) is a prooxidant and carcinogen, inducing thyroid tumors. Melatonin and indole‐3‐propionic acid (IPA) are effective antioxidants. Some antioxidative effects of propylothiouracil (PTU)—a thyrostatic drug—have been found. The aim of the study was to compare protective effects of melatonin, IPA, and PTU against lipid peroxidation in the thyroids, collected from rats treated with KBrO3, and in homogenates of porcine thyroids, incubated in the presence of KBrO3. Wistar rats were administered KBrO3 (110 mg/kg b.w., i.p., on the 10th day of the experiment) and/or melatonin, or IPA (0.0645 mmol/kg b.w., i.p., twice daily, for 10 days), or PTU (0.025% solution in drinking water, for 10 days). Homogenates of porcine thyroids were incubated for 30 min in the presence of KBrO3 (5 mM) plus one of the antioxidants: melatonin (0.01, 0.1, 0.5, 1.0, 5.0, 7.5 mM), or IPA (0.01, 0.1, 0.5, 1.0, 5.0, 7.5, 10.0 mM), or PTU (0.01, 0.1, 0.5, 1.0, 5.0, 7.5, 10.0 mM). The level of lipid peroxidation products (MDA + 4‐HDA) was measured spectrophotometrically in thyroid homogenates. In vivo pretreatment with either melatonin or with IPA or with PTU decreased lipid peroxidation caused by KBrO3—injections in rat thyroid gland. Under in vitro conditions, PTU (5.0, 7.5, and 10.0 mM), but neither melatonin nor IPA, reduced KBrO3‐related lipid peroxidation in the homogenates of porcine thyroids. In conclusion, melatonin and IPA may be of great value as protective agents under conditions of exposure to KBrO3. © 2005 Wiley‐Liss, Inc.
Career choices and workplace challenges for individuals with ADHD
Authors: Nadeau Kathleen G. 
Published: February 2005

AbstractAttention deficit hyperactivity disorder (ADHD) in adults is increasingly recognized as a common disorder that can significantly impact workplace functioning, yet few clinicians or career consultants have training in how to help adults select careers or jobs within a chosen career that are a good match for their strengths, weaknesses, ADHD tendencies, and coexisting conditions. Straightforward career counseling is inadequate to meet the needs of adults who have ADHD, and significant follow‐up, brain‐based counseling or therapy is often necessary to help these adults implement the recommendations generated by an ADHD career assessment. This article guides the clinician through the multiple steps of clinical interview, assessment, and intervention when working with adults who have ADHD who face challenges in the workplace. © 2005 Wiley Periodicals, Inc. J Clin Psychol/In Session 61: 549–563, 2005.
Ileal digestibility of defatted soybean, lupin and chickpea seed meals in cannulated Iberian pigs: I. Proteins
Authors: Rubio Luis A 
Published: February 2005

AbstractFive castrated male Iberian pigs (100 ± 2 kg body weight) fitted with T‐shaped cannulas at the terminal ileum were used to determine ileal digestibility of legume seed meals. The diets were based on defatted soybean, lupin or chickpea seed meals and contained similar levels of digestible energy (14.2–15.1 kJ g−1) and protein (107 g kg−1). Protein‐free and a hydrolysed casein diets were used to study endogenous ileal amino acid flows. Chromium oxide (10 g kg−1 diet) was added to the diets as an indigestible marker. Ileal flows in pigs fed the hydrolysed casein diet were different (p < 0.05) in amino acid contents and composition from those on the protein‐free diet. Ileal sialic acid flows in pigs fed lupin‐ or chickpea‐based diets were higher (p < 0.05) than those of animals fed soybean or casein diets. Among essential amino acids, only the apparent ileal digestibilities of phenylalanine and valine in lupin meal were lower (p < 0.05) than those in soybean. Apparent ileal digestibilities of lupin aspartate and proline, together with chickpea aspartate, were also lower (p < 0.05) than those of soybean. True ileal digestibility of nitrogen in pigs fed lupin or chickpea meals, calculated according to values from animals fed the protein‐free diet, was lower (p < 0.05) than that for soybean or casein. Among individual essential amino acids, only the true ileal digestibility of phenylalanine in lupin was lower (p < 0.05) than that in soybean. True ileal digestibility of nitrogen calculated according to values obtained with pigs fed a hydrolysed casein diet was not different among soybean, lupin or chickpea meals. Among essential amino acids, only the true ileal digestibilities of isoleucine and lysine in chickpea were lower (p < 0.05) than those of soybean. It is concluded that true ileal nitrogen and amino acid digestibilities of lupin and chickpea meals are comparable to those of defatted soybean in Iberian pigs. The results with protein‐free diets tended to underestimate endogenous protein secretion in pigs fed on diets containing protein. Copyright © 2005 Society of Chemical Industry