bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Event Stimulation on Consumers' Purchase Behavior with Health Agricultural Products in the Digital Era: A Case Study of the Selenium-enriched Fraud
Authors: Song Zhenjiang , Luo Xin , Wu Baoshu , Zhu Shubin 
Published: October 2023

Abstract
Background: Understanding consumers' behavioral choices after the stimulus of negative events has great significance for repairing consumer perceptions, improving crisis management strategies for negative events, and promoting the food industry’s healthy development.
Methods: Based on the "stimulus-organic-response" (SOR) theory, this study constructed a consumer purchasing-behavior model stimulated by negative events and analyzed the influence mechanism of internal stimulus, external stimulus, and consumer characteristics on consumers' negative emotions and selenium-enriched agricultural products purchasing behavior.
Conclusions: The empirical analysis produced the following conclusions. ① Negative perceptions and media disclosure not only can directly predict consumers' purchasing behavior with Se-enriched agricultural products but can also predict consumers' purchasing behavior with Se-enriched agricultural products through the mediating effect of negative emotions. ②There is no effect of negative emotion between preferences and consumers' purchase of Se-enriched agricultural products. This is explained by the current online and offline purchase channels, which expand the space of consumer choice. Additionally, in the digital era, consumers are more likely to obtain product information and guidance from relatively professional or targeted opinions, and their purchasing preferences tend to be decentralized and personalized. The influence of lay belief, impulse buying, and herd behavior on consumer purchasing has gradually decreased. ③ External intervention cannot moderate the relationship between negative emotion and purchase. Findings indicated that in the digital era, the rise of we-media, the reduction of information-acquisition cost, and the rise of anti-authority thoughts all lead consumers to distrust authority interventions and consequently, they were more inclined to trust the opinions of non-institutional and non-academic opinion leaders such as celebrities with massive followings.
Comprehensive analysis of genomic complexity in the 5’ end coding region of the DMD gene in patients of exons 1-2 duplications based on long-read sequencing
Authors: Shen Jiandong , Ding Taoli , Sun Xueping , Yang Ji , Zhang Yue , Wang Jing , Ge Mengdi , Xu Heng , Xie Jiazi , Wang Fei , Diao Feiyang 
Published: October 2023

Abstract
Backgroud
Dystrophinopathies are the most common X-linked inherited muscle diseases, and the disease-causing gene is DMD. Exonic duplications are common type of pathogenic variants in DMD gene, however, 5’ end exonic duplications containing exon 1 are less common. When assessing the pathogenicity of exonic duplications in the DMD gene, consideration must be given to their impact on the reading frame. Traditional molecular methods, such as multiplex ligation-dependent probe amplification (MLPA) and next generation sequencing (NGS), are commonly used in clinic. However, they cannot discriminate the precise physical locations of breakpoints and structural features of genomic rearrangement. Long-read sequencing (LRS) can effectively overcome this limitation.
Results
We report three cases involving the duplications of exon 1 and/or exon 2 in DMD gene. The locations of the breakpoints and the characteristics of structural variants (SVs) are identified through LRS, enabling the classification of the variants' pathogenicity. Two distinct variant types encompassing exon 1 in the DMD Dp427m isoform and/or Dp427c isoform are identified, which have been infrequently reported previously. The male individuals harboring duplication variant of consecutive exons 1–2 in the DMD canonical transcript (Dp427m) and exon 1 in the Dp427c transcript are normal, indicating the variant is benign. However, one patient carries a complex SVs involving exon 1 of the DMD Dp427c transcript showing an obvious phenotype.
Conclusions
Our research sheds light on the complexity of DMD variants encompassing Dp427c/Dp427m promoter regions and emphasizes the importance of cautious interpretation when assessing the pathogenicity of DMD 5' end exonic duplications, particularly in carrier screening scenarios without an affected proband.
Cross-shore parallel tidal channel systems formed by alongshore currents
Authors: Xu Fan , Zhou Zeng , Wei Yizhang , Geng Liang , Zhang Ying , Gu Yuxian , Finotello Alvise , D'Alpaos Andrea , Gong Zheng , Zhang Changkuan , Coco Giovanni 
Published: October 2023

Abstract

Tidal channel systems arising from morphodynamic interactions exhibit a suite of diverse morphological configurations. A prevalent type is represented by linear dendritic channels formed by single-thread streams aligned roughly parallel or subparallel to each other in the cross-shore direction. Despite their ubiquity, the processes driving the formation of these parallel channel systems remain elusive. We conducted a morphological analysis of 275 parallel tidal channels from 20 different locations worldwide and found that the angle between individual parallel branches and the parent stream, from which they develop, consistently falls within the range of 80-100º. We employed numerical modeling to shed light on the underlying mechanisms governing their formation, revealing that alongshore uniformity in bed topography and the strength of tidal currents condition the alignment of parallel channels. Cross-shore parallel channels can be formed by alongshore tidal currents, and the channel orientation is largely governed by the shape of the bed profile. Straight and shore-normal parallel branches tend to form where the bed profile changes sharply around the mean sea level, while linearly sloping profiles lead to oblique parallel branches. By unraveling the physics underlying the formation of these striking but poorly understood geomorphic features, our results bear significant ramifications for the understanding and management of valuable tidal ecosystems.
A new evidence base to document millennial changes in coastal vegetation
Authors: Foster Nicole , Jones Alice , Serrano Oscar , Lafratta Anna , Lavery Paul , Dijk Kor-jent Van , Biffin Ed , Gillanders Bronwyn , Young Jennifer , Masque Pere , Gadd Patricia , Jacobsen Geraldine , Zawadzki Atun , Greene Andria , Waycott Michelle 
Published: October 2023

Abstract
Anthropogenic activities are causing detrimental changes to coastal plants– namely seagrass, mangrove, and tidal marsh. Looking beyond recent times and to past vegetation dynamics is critical to assess the response and resilience of an environment to change. Here, we develop a novel multi-proxy approach, providing a new evidence base to decipher long-term change in coastal plant communities. Combining targeted environmental DNA analysis with chemical analysis of soils, we reconstructed 4,000 years of change at a temperate wetland and identified an ecosystem shift that occurred between ~ 1000 and 2000 years ago. What was a previous subtidal seagrass system shifted to an intertidal mangrove environment at present. We demonstrate that high-resolution information on millennial changes in coastal vegetation can be attained through these proxies. This approach could be applied to other ecosystems to improve the way we protect, conserve, and restore vegetated ecosystems.
PKC beta inhibitor prevents renal ischaemia‒reperfusion injury by affecting macrophage infiltration and polarization
Authors: Li Chun Yan , Guo Shuiming , Xiao Ting , Chen Ying , Shushakova Nelli , Haller Hermann , Tian Mei , Rong Song 
Published: October 2023

Abstract

Background
Acute kidney injury (AKI) secondary to renal ischemia reperfusion injury (RIRI) continues to be a significant perioperative problem and there is no effective treatment.
We previously reported that proteinkinase (PKC) β inhibitor could attenuate RIRI after kidney transplantation in rats. However, the mechanism by which PKC β inhibitor protects against RIRI remains unclear.
Methods
RIRI [Ed1] model rats were subjected to right nephrectomy and clipping of the left renal pedicle for 60 minutes. The PKC β inhibitor was orally administered the day before surgery. The rats were sacrificed 24 hours after the operation, and blood and kidney samples were collected. Renal function, histomorphology, renal tubular injury marker KIM-1，renal papillary injury indicator RPA-1，macrophage subtype markers and inflammatory cytokines were analysed.
Results
The results showed that the PKCβ inhibitor had a protective effect against RIRI, as indicated by the attenuation of renal dysfunction and a reduction in renal injury. In addition, the PKC β inhibitor significantly reduced the expression of the M1 macrophage marker CD197 and increased the expression of the M2 macrophage marker CD163[Ed2] . The expression of the proinflammatory cytokines iNOS and IL-12 was downregulated, while the expression of the anti-inflammatory cytokines Arg-1 and Dectin-1 was upregulated by the PKCβ inhibitor.
Conclusions
The PKCβ inhibitor can alleviate RIRI in rats to some extent, which may be related to improve tubular damage in ischemic renal tissue and promoting the polarization of macrophages to the M2 phenotype.
Prediction for incremental damage on final optics in ICF high power laser facility
Authors: Hu Xueyan , Zhou Wei , Guo Huaiwen , Huang Xiaoxia , Zhao Bowang , Zhong Wei , Zhu Qihua , Chen Zhifei 
Published: October 2023

Abstract
High power laser facility requires prediction for incremental damage on final optics in order to find varying damage developing trends, then in terms of the trends we can appropriately formulate maintain plans for long-term physical experiment operation on the facility. This essay indicates an image process technology via deep learning. By comparing fit offline optics image with online optics image, the authenticity and the area of the damage can be correctly defined. Besides, due to the comparison we can establish a damage detection model. Through appropriate algorithm and image matching technology, we could manage element damages on the timeline. And the damage varying trends could be found when additionally combined experimental parameter with analysis on specific damage area. Thus the surplus value of assembly can be optimized. By programming a software for elements management this technology could be used on high power laser facility.
DDA1, a novel factor in transcription-coupled repair, modulates CRL4CSA dynamics at DNA damage-stalled RNA polymerase II
Authors: Pines Alex , Schiffmacher Diana Llerena , Lee Shun-Hsiao , Kliza Katarzyna , Theil Arjan , Akita Masaki , Helfricht Angela , Bezstarosti Karel , Gonzalo-Hansen Camila , Attikum Haico van , Vries Matty Verlaan-de , Vertegaal Alfred , Hoeijmakers Jan , Marteijn Jurgen , Lans Hannes , Demmers Jeroen , Vermeulen Michiel , Sixma Titia , Ogi Tomoo , Vermeulen Wim 
Published: October 2023

Abstract

Transcription-blocking DNA lesions are specifically targeted by transcription-coupled nucleotide excision repair (TC-NER), which removes a broad spectrum of DNA lesions to preserve transcriptional output and thereby cellular homeostasis to counteract aging. TC-NER is initiated by the stalling of RNA polymerase II at DNA lesions, which triggers the assembly of the TC-NER-specific proteins CSA, CSB and UVSSA. CSA, a WD40-repeat containing protein, is the substrate receptor subunit of a cullin-RING ubiquitin ligase complex composed of DDB1, CUL4A/B and RBX1 (CRL4CSA). Although ubiquitination of several TC-NER proteins by CRL4CSA has been reported, it is still unknown how this complex is regulated. To unravel the dynamic molecular interactions and the regulation of this complex, we applied a single-step protein-complex isolation coupled to mass spectrometry analysis and identified DDA1 as a CSA interacting protein. Cryo-EM analysis showed that DDA1 is an integral component of the CRL4CSA complex. Functional analysis revealed that DDA1 coordinates ubiquitination dynamics during TC-NER and is required for efficient turnover and progression of this process.
Cerevisterol and aloesol from a marine derived Penicillium fungus as potential topical cosmeceutical ingredients
Authors: Hoang Chi K. , Tran Ha T.H. , Tran Hang T.N. , Hoang Diep T. , Le Cuong H. 
Published: October 2023

Abstract
• Background
The use of natural products in cosmetics and pharmacy has dramatically increased in recent years, leading to the overexploitation of flora and fauna worldwide and threatening environmental sustainability. Microbial-derived components could help solve this problem owing to their independently controllable cultural properties.
• Results
For the purpose of investigating microfungi for producing potential novel cosmeceuticals, cerevisterol (1) and aloesol (2) were isolated from a Penicillium fungus and tested for biological properties, including antimicrobial, tyrosinase inhibitory and wound healing activities. The results revealed wound-healing potentials of two fungal compounds (1) and (2) in terms of cell proliferation promotion in NIH-3T3 murine fibroblasts and scratch assay on 24-well plates using SPLScar™ Scratcher. Interestingly, the filamentous fungus-originated cerevisterol exhibited antimicrobial activity against the acne-causing bacteria Propionibacterium acnes and an inhibitory effect against tyrosinase in substrate L-tyrosine with IC50 = 51.98 µM. In addition, (1) and (2) were found to enhance the wound closure rates of the fibroblast monolayer after 72 h of incubation by up to 19.86–24.82% compared to the vehicle control.
• Conclusions
The results opened new prospects for the application of filamentous fungus-derived compounds, especially in the cosmetics industry.
Causality of Genetically Determined Monounsaturated Fatty Acids on Risk of Cardiovascular Disease: A Mendelian Randomization Study
Authors: Mansourian Marjan , Habibi Danial , Akbarzadeh Mahdi , Teymoori Farshad , Fateh Sahand Tehrani , Masjoudi Sajedeh , Saeidian Amir Hossein , Hosseinpanah Farhad , Mosavi Noushin , Hakonarson Hakon , Azizi Fereidoun , T. Alireza Soleymani , Hedayati Mehdi , Daneshpour Maryam 
Published: October 2023

Abstract
Background/Aim:
We performed the Mendelian randomization (MR) analysis to determine the causal relationship of serum monounsaturated fatty acids (MUFAs) with the risk of various cardiovascular diseases (CVDs).
Method:
The summary statistics dataset related to serum MUFAs was used from the published GWAS of European descent in UK Biobank participants (N=114,999). Genetic variants underlying angina, atherosclerotic, IHD, MI, and BP events were ascertained using a GWAS dataset of 461,880 (case= 14,828, control= 447,052), 463,010 (case= 12,171, control= 450,839), 361,194 (case= 20,857, control= 340,337), 462,933 (case= 10,616, control= 452,317), and 461,880 (case= 124,227, control= 337,653) European descent participants from the UK Biobank, respectively.
Results:
Our results show that MUFAs were associated with angina [ORIVW= 1.005, 95% CI: 1.003–1.007, p = <0.001; Cochran's Q=23.89, p=0.717, I2=0.0%; Egger intercept= -0.0003, p=0.289], atherosclerotic [ORIVW= 1.005, 95% CI: 1.003–1.007, p = <0.001; Cochran's Q=42.71, p=0.078, I2=27.4%; Egger intercept= -0.0004, p=0.146], IHD [ORIVW= 1.004, 95% CI: 1.001–1.007, p = 0.005; Cochran's Q=42.75, p=0.172, I2=18.1%; Egger intercept= -0.0001, p=0.827], MI [ORIVW= 1.001, 95% CI: 0.999–1.003, p = 0.199; Cochran's Q= 23.03, p=0.631, I2=0.0%; Egger intercept= -0.0003, p=0.196], and BP [ORWM= 1.008, 95% CI: 1.001–1.015, p = 0.022; Cochran's Q= 52.87, p=0.015, I2= 37.6%; Egger intercept= 0.0002, p=0.779]. These results remained consistent using different MR method and sensitivity analyses.
Conclusion:
These findings prompt significant questions about the function of MUFAs in the progression of CVD events. Further research is required to elucidate the connections between MUFAs and CVD to contribute to health policy decisions in reducing CVD risk.
Diverse Cellular Tropisms and Immune Responses during Beta-Coronavirus Infections in Human Bronchial Epithelial Organoids
Authors: Choi Young Ki , Park Dongbin , Kim Se-Mi , JANG HOBIN , Kim Kanghee , Ji Ho young , Yang Heedong , Kwon Woohyun , Kang Yeonglim , Hwang Suhee , Kim Hyunjoon , Lee Joo-Yeon 
Published: October 2023

Abstract
The lower respiratory system serves as the primary target and barrier for beta-coronavirus (beta-CoV) infections. We investigated the interactions between four distinct beta-CoVs [HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2 (Omicron)] in human bronchial epithelial (HBE) organoids using single-cell RNA sequencing (scRNA-seq). Our findings revealed diverse viral tropisms: SARS-CoV and SARS-CoV-2 mainly infected ciliated cells, while HCoV-OC43 and MERS-CoV preferred club and goblet cells, respectively. Notably, genes encoding the host receptor BSG (CD147) and proteases (TMPRSS2, cathepsin B/D, and SPCS1) associated with beta-CoV infections were upregulated by all four virus infections, particularly in club cells. Transcriptomic profiling showed that these viruses commonly induced inflammation via TNF-α/NF-кB signaling and reduced interferon responses. However, host-defense pathways like the unfolded protein response, ER-related calcium regulation, and oxidative stress response exhibited cell-type and virus-dependent regulations. Notably, in contrast to SARS-CoV infection, HCoV-OC43 infected cells induce the upregulation of IFNα-related interferon-stimulated genes (ISGs) particularly in club and goblet cells, potentially suppressing viral replication despite widespread infection. Taken together, our study redefines our understanding of beta-CoVs cellular tropism and highlights the intricate interplay between these cells and the host's immune defense mechanisms.
Analysis of the china tuberculosis incidence study from 2004 to 2019 and association with GDP per capita: A Frontier Analysis
Authors: Lv Hengliang , Li Xuan , Zhang Xin , Wang Longhao , Zhang Xueli , Bai Junzhu , You Shumeng , Zhang Wenyi , Xu Yuanyong 
Published: October 2023

Abstract
Background: Tuberculosis (TB) continues to pose a considerable social and economic burden, with China currently ranking as the third highest in terms of TB burden globally. This study examined the variations in TB incidence across different provinces in China between 2004 and 2019, while analyzing its relationship with Gross Domestic Product (GDP) per capita. The objective is to provide more precise insights to aid the formulation of effective policies for TB prevention and control in China.
Method: The TB data utilized in this study was obtained from the Data-centre of China Public Health Science. To analyze the temporal trends of TB incidence rate from 2004 to 2019, joinpoint regression analysis was employed. In order to estimate the overall level of spatial autocorrelation, global spatial autocorrelation was utilized, and local indicators of spatial association were employed to assess the impact of individual locations on the magnitude of the global statistic and to identify the location and types of clusters. Additionally, to assess the relationship between the incidence of TB and GDP per capita, Pearson's test and frontier analysis were adopted.
Results: The analysis revealed a consistent decline in TB incidence from 2004 to 2019(APC = -3.2, P < 0.001), with the highest incidence occurring in spring and the lowest in December each year. Significant global spatial autocorrelation existed in the TB incidence rate from 2004 to 2019 (all P < 0.005) with the Moran’s I index ranging from 0.1926 to 0.5319. The western regions of China primarily exhibited a high incidence, forming the high-high cluster, whereas Beijing, Tianjin, Hebei, and Jiangsu consistently belonged to the low-low cluster throughout the study period. We discovered a correlation between TB incidence and per capita GDP, and some regions with inadequate TB control, such as Tibet, Xinjiang, Qinghai and Guizhou, etc provinces, exhibited higher incidence rates that given GDP per capita.
Conclusion: Tibet, Xinjiang, Qinghai and Guizhou, etc show a larger TB burden compared to other regions with similar GDP per capita, which requires further exploring how to control TB in a satisfactory situation with limited capital investment and health resources.
A Real-World Cohort Study of First-Line Afatinib in Patients with EGFR-Mutant Advanced Non-Small Cell Lung Cancer in Vietnam
Authors: Pham Cam Phuong , Nguyen Thi Thai Hoa , Do Anh Tu , Nguyen Tuan Khoi , Hoang Thi Anh Thu , Le Tuan Anh , Vuong Dinh Thy Hao , Nguyen Dac Nhan Tam , Dang Van Khiem , Nguyen Thi Oanh , Pham Van Luan , Vo Thi Huyen Trang , Do Hung Kien , Vu Ha Thanh , Trinh Le Huy , Nguyen Khac Dung , Nguyen Hoang Gia , Truong Cong Minh , Pham Tran Minh Chau , Pham Van Thai , Nguyen Thi Thuy Hang , Nguyen Minh Hai , Nguyen Thi Bich Phuong 
Published: October 2023

Abstract
Background: This study aimed to evaluate the efficacy and side effects of first-line afatinib treatment in a real-world setting in Vietnam.
Methods: This retrospective study was conducted across nine hospitals in Vietnam. Advanced epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC) patients who received afatinib as first-line therapy between April 2018 and June 2022 were included, and patient medical records were reviewed. Key outcomes were overall response rate (ORR), time-to-treatment failure (TTF), and tolerability.
Results: A total of 343 patients on first-line afatinib were eligible for the study. EGFR exon 19 deletion (Del19) alone was detected in 46.9% of patients, L858R mutation alone in 26.3%, and other uncommon EGFR mutations, including compound mutations, in 26.8%. Patients with brain metastases at baseline were 25.4%. Patients who received 40 mg, 30 mg, and 20 mg as starting doses of afatinib were 58.6%, 39.9%, and 1.5%, respectively. The ORR was 78.1% in the overall population, 82.6% in the Del19 mutation subgroup, 73.3% in the L858R mutation subgroup, and 75.0% in the uncommon mutation subgroup (p>0.05). The univariate and multivariate analyses indicate that the ORR increased when the starting dose was 40 mg compared to starting doses below 40 mg (83.9% vs 74.3%, p=0.034). The median TTF (mTTF) was 16.7 months (CI 95%: 14.8 – 18.5) in all patients, with a median follow-up time of 26.2 months. The mTTF was longer in patientsin the common EGFR mutation subgroup (Del19/L858R) than in those in the uncommon mutation subgroup (17.5 vs 13.8 months, p=0.045) and in those without versus with brain metastases at baseline (17.5 vs 15.1 months, p=0.049). There were no significant differences in the mTTF between subgroups based on the starting dose of 40 mg and <40 mg (16.7 vs 16.9 months, p>0.05). The most common treatment-related adverse events (any grade/grade ≥3) were diarrhea (55.4%/3.5%), rash (51.9%/3.2%), paronychia (35.3%/5.0%), and stomatitis (22.2%/1.2%).
Conclusions: Afatinib demonstrated clinical effectiveness and good tolerability in Vietnamese EGFR-mutant NSCLC patients. In our real-world setting, administering a starting dose below 40 mg might result in a reduction in ORR; however, it might not have a significant impact on TTF.