bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring Piezo1, Piezo2, and TMEM150C in Human Brain Tissues and Their Correlation with Brain Biomechanical Characteristics
Authors: Raha Arjun , Wu Yuning , Zhong Lily , Raveenthiran Jatheeshan , Hong Minji , Taiyab Aftab , Wang Li , Wang Bill , Geng Fei 
Published: October 2023

Abstract
Unraveling the intricate relationship between mechanical factors and brain activity is a pivotal endeavor, yet the underlying mechanistic model of signaling pathways in brain mechanotransduction remains enigmatic. To bridge this gap, we introduced an in situ multi-scale platform, through which we delineate comprehensive brain biomechanical traits in white matter (WM), gray-white matter junctions (GW junction), and the pons across human brain tissue from four distinct donors. We investigate the three-dimensional expression patterns of Piezo1, Piezo2, and TMEM150C, while also examining their associated histological features and mechanotransduction signaling networks, particularly focusing on the YAP/β-catenin axis. Our results showed that the biomechanical characteristics (including stiffness, spring term, and equilibrium stress) associated with Piezo1 vary depending on the specific region. Moving beyond Piezo1, our result demonstrated the significant positive correlations between Piezo2 expression and stiffness in the WM. Meanwhile, the expression of Piezo2 and TMEM150C was shown to be correlated to viscoelastic properties in the pons and WM. Given the heterogeneity nature of brain tissue, we investigated the three-dimensional expression of Piezo1, Piezo2, and TMEM150C. Our results suggested that three mechanosensitive proteins remained consistent across different vertical planes within the tissue sections. Our findings not only establish Piezo1, Piezo2 and TMEM150C as pivotal mechanosensors that regulate the region-specific mechanotransduction activities, but also unveil the paradigm connecting brain mechanical properties and mechanotransduction activities and the variations between individuals.
Comparison of robot versus fluoroscopy-assisted pedicle screw instrumentation in adolescent idiopathic scoliosis surgery: A Meta- Analysis
Authors: Wei LinDong , Duan YuChen , Huo JianZhong 
Published: October 2023

Abstract

Aim
To investigate the safety and accuracy of robot-assisted pedicle screw placement in adolescent idiopathic scoliosis (AIS) surgery.
Methods
PubMed, Medline, Embase, Cochrane Library, Web of Science, CNKI, and EMCC databases were searched for studies utilizing the clinical data of adolescent idiopathic scoliosis patients who underwent posterior pedicle screw fixation to collect preoperative, postoperative and follow-up indexes and imaging parameters, including screw implantation accuracy, the number of intraoperative fluoroscopy times, amount of bleeding, operation time, postoperative VAS score and postoperative Cobb angle.
Results
Six studies were included in this meta-analysis, including 154 patients in the intervention group and 171 patients in the control group. Our results showed that screw implantation accuracy (WMD 1.07, 95% CI (1.01,1.14), P = 0.03) was higher than that in the control group. The number of intraoperative fluoroscopy times (WMD − 6.11, 95% CI (-9.60, -2.62), P = 0.0006) and operation time (WMD 31.52, 95% CI (5.70,57.35), P = 0.02) in the robot group were less, and the differences were statistically significant. Intraoperative blood (WMD − 59.05, 95% CI (-212.81,94.70), P = 0.45), postoperative VAS score (WMD − 0.07, 95% CI (-0.24,0.11), P = 0.46), postoperative Cobb angle (WMD 0.79, 95% CI (-1.12,2.69), P = 0.42) had no statistical significance in the robot group compared with control group.
Conclusion
The robot navigation system can improve screw implantation accuracy and reduce the operation time and radiation exposure during the operation.
One-year outcome of intense pulsed light therapy for refractory meibomian gland dysfunction in patients continuously exposed to hypotensive agents: a comparative study
Authors: Fan Nai-Wen , Yang Chi-Chun , Ko Yu-Chieh , Chen Meng-Jou 
Published: October 2023

Abstract

Background: To compare the one-year effect of intense pulsed light (IPL) for refractory meibomian gland dysfunction (MGD) between patients treated with and without topical hypotensive agents.
Methods: This comparative retrospective cohort study included 41 MGD eyes of glaucoma patients (age, 67.59±11.12 years) and 41 MGD eyes of non-glaucoma patients (age, 62.73±15.97 years). Both arms received a course of IPL with meibomian gland (MG) expression (MGX). Baseline characteristics were matched by propensity score test. Standard evaluation including eye dryness (SPEED) questionnaire, tear breakup time (TBUT), lid telangiectasia, corneal fluorescein staining (CFS) score, meibum quality and expressibility were assessed at baseline, 3, 6, 9 and 12 months after treatment.
Results: No baseline intergroup heterogeneity was detected after matching. Both groups demonstrated significant improvements in SPEED score (-6.37 vs -3.23), lid telangiectasia (-0.62 vs -0.60), MG expressibility (-0.66 vs -0.93) and meibum quality (-0.83 vs -0.84) at 6 months (all p < 0.01), with a sustainable effect up to 9 months in glaucoma group and 12 months in non-glaucoma group in terms of SPEED score and MG expressibility. Both arms showed significant improvements in TBUT and meibum quality at 12 months (p < 0.01). CFS significantly decreased in the non-glaucoma group at 6 months while there was no significant change of CFS in glaucoma group over the follow-up period.
Conclusions: IPL with regular MGX exerts a promising sustainable effect in ameliorating symptoms and signs of MGD patients treated with topical hypotensive agents, but the effect lasts shorter than that in non-glaucoma patients.
Metadichol-induced differentiation of pancreatic ductal cells (PANC-1) into insulin-producing cells
Authors: Raghavan Palayakotai R 
Published: October 2023

Abstract

Endocrine gene expression in PANC-1, a type of pancreatic cancer cell, has been studied in the context of their potential to be reprogrammed toward a normal, differentiated state. Alkaline phosphatase activity has also been shown in pluripotent stem cells to differentiate between feeder and parental cells in reprogramming experiments. Metadichol®-based cell programming holds promise as a versatile and potentially safer approach for manipulating cellular behavior without the use of viral vectors, gating, or CRISPR. This study investigated the multifold increase in the gene expression of CA9, GCG, INS MAFA, NEUROD1, NGN3, NKX2-2, PAX6: PDX1, SLC2A2, FOXO1, and SIRT1 using qRT‒PCR. These genes regulate endocrine cell development in the pancreas and are involved in insulin and glucagon secretion. Gene network analysis is presented to show how Metadichol-induced expression leads to a closed loop feedback network and biological process that would help in mitigating diabetes and other related disorders.
Implementation of remote sensing algorithms to estimate TOC, Chl-a and TDS in a tropical water body; Sanalona reservoir, Sinaloa, Mexico
Authors: Quevedo-Castro Alberto , Monjardín-Armenta Sergio Alberto , Plata-Rocha Wenseslao , Rangel-Peraza Jesus Gabriel 
Published: October 2023

Abstract
The present study implements a methodology to estimate water quality values using statistical tools and remote sensing techniques in a tropical water body Sanalona. Linear regression models developed by Box-cox transformations and processed metadata from LANDSAT-8 imagery (bands) were used to estimate TOC, TDS, and Chl-a of the Sanalona reservoir from 2013 to 2020 at 5 sampling sites measured every six months. A band discriminant analysis was carried out to statistically fit and optimize the proposed algorithms. Coefficients of determination beyond 0.9 were obtained for these water quality parameters (r2TOC = 0.90, r2TDS = 0.95 and r2Chl − a = 0.96). The validation of these proposed models was carried out by comparing the estimated values of TOC, TDS, and Chl-a with the historical database provided by CONAGUA. The present study implemented, validated, and compared the results obtained by using an ordered and standardized methodology proposed for the estimation of TOC, TDS, and Chl-a values based on water quality parameters measured in the field and using satellite images.
Identifying functional subtypes and common mechanisms of rheumatoid arthritis and systemic lupus erythematosus
Authors: Li Jiajun , Chen Rui , Shang Zhenwei , Song Zerun , Li Shuai , Meng Xin , Cheng Xiangshu , Tang Hao , Lv Wenhua , Zhang Ruijie 
Published: October 2023

Abstract
Background
Although there has been much research on Rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), few studies focus on the classification and commonality of the two diseases. Therefore, we intends to conduct a joint subtype analysis of RA and SLE based on molecular and immune characteristics, and to investigate the similarity between RA and SLE and heterogeneity between subtypes of the two diseases.
Results
We analyzed the proportion differences of immune cell subpopulations and identified cell type specific expressed genes based on single-cell RNA sequencing data. Using these genes of abnormal proportion cells as as marker genes for classification, 232 RA patients and 160 SLE patients with bulk RNA sequencing data were grouped into two subtypes by a consensus clustering algorithm. The marker genes showed different expression patterns in two subtypes, and each of the subtype contained both RA and SLE patients. Then we estimated the composition of the immune microenvironment through ssGSEA and Cibersort algorithms, and analyzed the clinical characteristics of RA patients, which verified the heterogeneity between different subtypes. Next, we identified three co-expression modules highly correlated with subtypes by using WGCNA algorithm, and screened for differentially expressed genes (DEGs) between the two disease subtypes. To furter explore the biological mechanisms of different subtypes, functional enrichment analysis of modular genes and DEGs was performed. SubtypeⅠis associated with abnormal activation of phagocytic cells caused by bacterial infection, while subtypeⅡis associated to abnormal activation of lymphatic cells caused by viral infection. To verify accuracy of subtype classification and to test whether the marker genes can be used as subtype signatures and potential drug targets, we constructed LASSO and random forest models, got 10 marker genes between two subtypes, realized the precise subtype classification with average precision rate reaching 90%.
Conclusion
Two common subtypes of RA and SLE were identified with significant heterogeneity between subtypes, which may provide new insights for the precise diagnosis and treatment of RA and SLE.
Design of stable circular permutants of the GroEL chaperone apical domain
Authors: Melnik Tatiana N. , Majorina Maria A. , Vorobeva Daria E. , Nagibina Galina S. , Veselova Victoria R. , Glukhova Ksenia А. , Pak Marina A. , Ivankov Dmitry N. , Uversky Vladimir N. , Melnik Bogdan S. 
Published: October 2023

Abstract
Enhancing protein stability holds paramount significance in biotechnology, therapeutics, and the food industry. Circular permutations offer a distinctive avenue for manipulating protein stability while keeping intra-protein interactions intact. Amidst the creation of circular permutants, determining the optimal placement of the new N- and C-termini stands as a pivotal, albeit largely unexplored, endeavor. In this study, we employed PONDR-FIT’s predictions of disorder propensity to guide the design of circular permutants for the GroEL apical domain (residues 191–345). Our underlying hypothesis posited that a higher predicted disorder value would correspond to reduced stability in the circular permutants, owing to the increased likelihood of fluctuations in the novel N- and C-termini. To substantiate this hypothesis, we engineered six circular permutants, positioning glycines within the loops as locations for the new N- and C-termini. We demonstrated the validity of our hypothesis along the set of the designed circular permutants, as supported by measurements of melting temperatures by circular dichroism and differential scanning microcalorimetry. Consequently, we propose a novel computational methodology that rationalizes the design of circular permutants with projected stability.
Evaluation of Prompt Engineering Strategies for Pharmacokinetic Data Analysis with the ChatGPT Large Language Model
Authors: Shin Euibeom , Ramanathan Murali 
Published: October 2023

Abstract
Purpose
To systematically assess the ChatGPT large language model on diverse tasks relevant to pharmacokinetic data analysis.
Methods
ChatGPT was evaluated with prototypical tasks related to report writing, code generation, non-compartmental analysis, and pharmacokinetic word problems. The writing task consisted of writing an introduction for this paper from a draft title. The coding tasks consisted of generating R code for semi-logarithmic graphing of concentration-time profiles and calculating area under the curve and area under the moment curve from time zero to infinity. Pharmacokinetics word problems on single intravenous, extravascular bolus, and multiple dosing were taken from a pharmacokinetics textbook. Chain-of-thought and problem separation were assessed as prompt engineering strategies when errors occurred.
Results
ChatGPT showed satisfactory performance on the report writing, code generation tasks and provided accurate information on the principles and methods underlying pharmacokinetic data analysis. However, ChatGPT had high error rates in numerical calculations involving exponential functions. The outputs generated by ChatGPT were not reproducible: the precise content of the output was variable albeit not necessarily erroneous for different instances of the same prompt. Incorporation of prompt engineering strategies reduced but did not eliminate errors in numerical calculations.
Conclusions
ChatGPT has the potential to become a powerful productivity tool for writing, knowledge encapsulation, and coding tasks in pharmacokinetic data analysis. The poor accuracy of ChatGPT in numerical calculations require resolution before it can be reliably used for PK and pharmacometrics data analysis.
Identification and validation of mitochondrial oxidative stress- related prognostic signature with clinical characters and immune filtration in liver hepatocellular carcinoma
Authors: Wang Zhihui , Zhou Hao , Zhang Lie , Liu Xin , Wang Hui 
Published: October 2023

Abstract

Background Mitochondrial oxidative stress has been shown to play a critical role in cancer development and progression. But there was relatively less research on the relationship between mitochondrial oxidative stress and LIHC. Methods Mitochondrial oxidative stress-related genes were collected from Genecards portals. Prognosis-linked genes (PLGs) were identified by univariate Cox regression analysis. A risk model was constructed based on PLGs using the least absolute shrinkage and selection operator (LASSO) analysis. The receiver operational feature (ROC) curve was used to detect the model’s prediction ability. The gene expression level of prognostic genes were verified in cell lines. Results We constructed a novel risk model on the basis of 9 prognostic genes (CYP2C19, CASQ2, LPL, TXNRD1, CACNA1S, SLC6A3, OXTR, BIRC5, and MMP1). The Kaplan-Meier survival analysis showed that patients with a low-risk score had a much better overall survival (OS) rate than those with a high-risk score. The prognostic risk score was determined to be an independent predictor of prognosis. Patients in the high-risk group had a less favorable tumor microenvironment, characterized by a lower degree of immune cell infiltration. In contrast, the low-risk group demonstrated a higher degree of immune cell infiltration, which could potentially contribute to a more effective anti-tumor immune response. Conclusion Our investigation uncovered the oncogenic role of mitochondrial oxidative stress in LIHC. For the first time, we established a risk prediction model for mitochondrial oxidative stress in LIHC.
Comparative analysis of the quality of execution of road surfaces on newly built, reconstructed and renovated roads in the city Płock area (Poland)
Authors: Gryszpanowicz Piotr , Gasik-Kowalska Natalia , Waluś Konrad J. 
Published: October 2023

Abstract
Carrying out repair works, reconstruction, and construction of new road surfaces is a permanent element of urban space. The quality of the new pavement for the adopted traffic category directly impacts the road infrastructure's durability. The choice of road surface structure depends on the adopted traffic category. The aim of the article is to assess the works carried out on selected road surfaces within the city of Płock (Poland) in terms of the technical specification requirements and the durability of road infrastructure. The paper presents the tests of three road layers: base layer, binding layer and wearing course. The tests were carried out on 11 streets, and 29 samples were collected.
Pulmonary function assessment after COVID-19 in vaccinated healthcare workers
Authors: Ippoliti Lorenzo , Coppeta Luca , Somma Giuseppina , Bizzarro Giuseppe , Borelli Francesco , Crispino Teresa , Ferrari Cristiana , Iannuzzi Ilaria , Mazza Andrea , Paolino Agostino , Magrini Andrea , Pietroiusti Antonio 
Published: October 2023

Abstract
Long COVID refers to persistent symptoms following SARS-CoV-2 infection for at least 2 months with no other clear cause. Symptoms can occur after initial infection or during recovery and include chest pain, fatigue, breathlessness and cough. The exact causes are being investigated and may involve organ damage, inflammation or an immune response. COVID-19 can cause severe respiratory damage and is different from classic ARDS. Radiological assessment is recommended for those with dyspnoea after COVID. Clinical outcomes are variable and permanent lung damage is not fully understood. Vaccination is effective against severe infection, but its effect on respiratory function in mild cases remains uncertain. This retrospective study aims to analyse changes in lung function in HCWs who had COVID-19 between 2020 and 2022, comparing their spirometric test results before and after the pandemic and taking into account their vaccination status. 321 HCWs were included in the study. The study examined spirometric parameters both before and after the pandemic, and all measured outcomes except the FEV1/FVC ratio showed a significant decrease during the study period. We then assessed the association between COVID-19 infection and changes in lung function parameters, analysing infections in 2020, 2021 and 2022 separately. We found a statistically significant difference in Forced vital capacity (FVC) between infected and non-infected subjects in 2020 and 2021, but not in 2022. To evaluate the protective effect of SARS-CoV-2 vaccination on respiratory function, a linear regression analysis was performed using changes in FVC, Forced expiratory volume in 1 second (FEV1), FVC/FEV1 ratio and Peak expiratory flow (PEF) as dependent variables. The analysis showed that the decline in FVC was significantly lower in subjects who had been vaccinated prior to infection. The study concludes that subclinical COVID-19 infections in 2020 and 2021 worsened respiratory parameters (FVC and FEV1), but vaccination protected against these effects. Even healthy individuals with previous infections showed respiratory changes, with vaccination providing protection, especially for FVC decline. This highlights the importance of vaccinating healthcare workers against COVID-19.
Strategies against the shortage of skilled welders
Authors: Schmelzer Aimée , König Anja 
Published: October 2023

Abstract
The global welder shortage is threatening industries worldwide, resuming in loss of revenue. Various research shows a range of approaches where the industry needs to improve, in order to attract and retain skilled welders. Researches were conducted amongst apprentices, welders and HR managers, in order to understand the current status and the future needs of the work force as well as the issues the industry currently is facing.
The study shows that there is a need for a holistic approach in order to solve the problem. Starting with dignity and appreciation of the work, evaluation of the workforce as a cost or value generator and social recognition as a base. The importance of welding should be more focused in the everyday life. In what work environment do most of the welders work with regards to safety, working hours, what about physical and mental stress. The industry needs to implement new work methods also in production, to be an attractive employer to skilled welders. This also includes agile working teams, well trained leaders that are giving trust and thus enabling creative freedom, seeing the employee as a human being with his or her own personality and individual needs, creating future-oriented work structures.
Clinical Application Of Fiberoptic Bronchoscopy Guided Awake Tracheal Intubation In Removal Of Internal Fixation For Cervical Spine Fracture: Case Report
Authors: Zeng Lin , Luo Tuo , Liu Yusong , Chen Yali 
Published: October 2023

Abstract
Background and objectives:
When planning the management of a predicted difficult airway, it is important to determine which strategy will be followed. Using fiberoptic bronchoscopy is a major option in scenarios with factors suggesting difficult airway access. It is also indicated in rescue situations, when there is tracheal intubation failure with direct laryngoscopy. The purpose of this report is to demonstrate the efficacy of using fiberoptic bronchoscopy as the preferred device for patients after cervical spine fracture surgery with almost no neck mobility and difficult airways.
Case report:
An 47 year-old famale patient, 168 cm, 65 kg, ASA Physical Status II, Mallampati IV classification, was scheduled for internal fixation removal surgery for cervical spine fractures. Although the patient had a difficult airway, she showed no signs of respiratory failure or airway obstruction. After entering the room, provide oxygen through a nasal catheter with a flow rate of 2 L/min. After establishing a venous pathway and monitoring, intravenous infusion of dexmedetomidine with a load of 0.5ug/kg was completed within 10 minutes. During airway preparation, always maintain the patient's autonomous breathing. The anesthesiologist performed a transcricoid puncture on the patient and injected 3mL of 2% lidocaine solution into the tracheal cavity. The whole mouth was sprayed with 2% lidocaine under the guidance of fiberoptic bronchoscope. An armored tube with a guide wire inside was used for tracheal intubation, performed on the first attempt with appropriate glottis visualization.
Conclusion
The fiberoptic bronchoscope occupies a prominent position in cases in which access to the airway is difficult. Fiberoptic bronchoscope guidance in awake endotracheal intubation for patients with difficult airway has the advantages of rapid, accurate, safe, high success rate and little damage. This can provide a new and effective method for the difficult airway management of these patients.