bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a liquid chromatography/tandem mass spectrometry assay for the quantification of PM00104, a novel antineoplastic agent, in mouse, rat, dog, and human plasma
Authors: Yin Jianming , Aviles Pablo , Lee William , Ly Carl , Guillen Maria Jose , Munt Simon , Cuevas Carmen , Faircloth Glynn 
Published: February 2005

AbstractA rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay was developed and validated to quantify a novel antineoplastic agent, PM00104, in mouse, rat, dog, and human plasma. The method was validated to demonstrate the specificity, limit of quantification (LOQ), accuracy, and precision of measurements. The calibration range for PM00104 was established using PM00104 standards from 0.01–5.0 ng/mL in blank plasma. The selected reaction monitoring (SRM), based on the m/z 692.2 → 218.2 transition, was specific for PM00104, and that based on the m/z 697.2 → 218.2 transition was specific for PM00104 (13C2,2H3) (the internal standard, IS); no endogenous materials interfered with the analysis of PM00104 and IS from blank plasma. The assay was linear over the concentration range 0.01–5.0 ng/mL. The correlation coefficients for the calibration curves ranged from 0.9981–0.9999. The mean intra‐day and inter‐day accuracies for all calibration standards (n = 8) ranged from 97–105% (≤5% bias) in human plasma, and the mean inter‐day precision for all calibration standards was less than 8.5%. The mean intra‐ and inter‐day assay accuracy for all quality control (QC) replicates in human plasma (n = 9), determined at each QC level throughout the validated runs, ranged from 96–112% (≤12% bias) and from 102–105% (≤5% bias), respectively. The mean intra‐ and inter‐day assay precision was less than 15.0 and 11.8% for all QC levels, respectively. For the QC samples prepared in animal species plasma, the %CV values of the assays ranged from 1.8–8.8% in mouse plasma, from 3.7–13.8% in rat plasma, and from 3.0–7.2% in dog plasma. The assay accuracies ranged from 92–102% (≤8% bias) for all QC levels prepared in mouse plasma; ranged from 93–106% (≤7% bias) in rat plasma; and ranged from 95–114% (≤14% bias) in dog plasma. The assay has been used to support preclinical pharmacokinetic and toxicokinetic studies and is currently used to measure PM00104 plasma concentrations to support clinical trials. Copyright © 2005 John Wiley & Sons, Ltd.
Surface heterogeneity of rat sperm during maturation
Authors: Cartwright Elizabeth J. , Harrington Pauline , Norbury Louise , Leeming Gareth , Sharpe Paul T. 
Published: February 2005

Rat sperm isolated from the caput and caudal epididymis and the vas deferens were subjected to multiple partition in aqueous two-phase systems. The technique was used to reveal heterogeneity of a sperm population with respect to particular surface properties. Sperm from all three regions gave broad distributions indicative of heterogeneous cell populations. Greatest heterogeneity was observed for cauda sperm with caput and was sperm producing similar distributions.
Following multiple partition sperm from different regions of the distribution profiles were immunostained with three antibodies known to recognise maturation antigens. The results show that some antigens are acquired during epididymal transit whilst others are present throughout.
The partition (surface heterogeneity) seen cannot therefore be explained solely by the distribution of the antigens recognised by 2D6, 6B2 and 3D5.
Vitamin D status in patients affected by Smith‐Lemli‐Opitz syndrome
Authors: Rossi M. , Federico G. , Corso G. , Parenti G. , Battagliese A. , Frascogna A. R. , della Casa R. , Dello Russo A. , Strisciuglio P. , Saggese G. , Andria G. 
Published: February 2005

SummarySmith‐Lemli‐Opitz syndrome (SLOS) is an inborn error of cholesterol biosynthesis characterized by developmental delay and multiple malformations.Some of the patients have skin photosensitivity and therefore tend to avoid direct exposure to sunlight.SLOS patients typically have low concentrations of cholesterol and abnormally high concentrations of its precursor 7‐dehydrocholesterol (7‐DHC) in biological fluids and tissues. 7‐DHC is also a precursor in the cutaneous synthesis of vitamin D. Sunlight exposure plays a major role in this pathway and reactions transforming 7‐DHC into vitamin D and then into 25‐hydroxyvitamin D are known not to be specifically regulated. The aim of this study was to evaluate vitamin D status in SLOS patients. We measured 25‐hydroxyvitamin D and 1,25‐dihydroxyvitamin D serum concentrations and markers of calcium metabolism in five SLOS patients.Despite abnormally high concentrations of 7‐DHC, circulating concentrations of vitamin D metabolites were not significantly different from appropriate controls matched for sex, age and season of blood collection. The analysis of historical serum samples stored in our laboratory from the same cases plus 10 other SLOS patients further supported these findings. Our data suggest that SLOS patients have a peculiar vitamin D metabolism that protects them from vitamin D intoxication.This appears to be due in most cases to decreased transformation of 7‐DHC into 25‐hydroxyvitamin D, perhaps depending on reduced sunlight exposure as a consequence of photosensitivity. Possible alternative mechanisms are discussed.
Promotion of oxidative stress by 3‐hydroxyglutaric acid in rat striatum
Authors: Latini A. , Scussiato K. , Leipnitz G. , Dutra‐Filho C. S. , Wajner M. 
Published: February 2005

SummaryThe pathophysiology of the striatum degeneration characteristic of patients affected by the inherited neurometabolic disorder glutaryl‐CoA dehydrogenase deficiency (GDD), also known as glutaric aciduria type I, is still in debate. We have previously reported that 3‐hydroxyglutaric acid (3‐OH‐GA) considered the main neurotoxin in this disorder, induces oxidative stress in rat cerebral cotex. In the present work, we extended these studies by investigating the in vitro effect of 3‐OH‐GA, at concentrations ranging from 0.01 to 1.0 mmol/L on the brain antioxidant defences by measuring total radical‐trapping antioxidant potential (TRAP), total antioxidant reactivity (TAR) and glutathione (GSH) levels, and on the production of hydrogen peroxide (H2O2), nitric oxide (NO) and malondialdehyde in striatum homogenates from young rats. We observed that TRAP, TAR and GSH levels were markedly reduced (by up to 50%) when striatum homogenates were treated with 3‐OH‐GA. In contrast, H2O2 (up to 44%), NO (up to 95%) and malondialdehyde levels (up to 28%) were significantly increased by 3‐OH‐GA. These data indicate that total nonenzymatic antioxidant defences (TRAP) and the tissue capacity to handle an increase of reactive species (TAR) were reduced by 3‐OH‐GA in the striatum. Furthermore, the results also reflect an increase of lipid peroxidation, probably secondary to 3‐OH‐GA‐induced free radical production. Thus, it may be presumed that oxidative stress is involved in the neuropathology in GDD.
Glutathione deficiency in patients with mitochondrial disease: Implications for pathogenesis and treatment
Authors: Hargreaves I. P. , Sheena Y. , Land J. M. , Heales S. J. R. 
Published: February 2005

SummaryGlutathione (GSH) is a key intracellular antioxidant. With regard to mitochondrial function, loss of GSH is associated with impairment of the electron transport chain (ETC). Since GSH biosynthesis is an energy‐dependent process, we postulated that in patients with ETC defects GSH status becomes compromised, leading to further loss of ETC activity. We performed electrochemical HPLC analysis to determine the GSH concentration of 24 skeletal muscle biopsies from patients with defined ETC defects compared to 15 age‐matched disease controls. Comparison of these groups revealed a significant (p <0.001) decrease in GSH concentration in the ETC‐deficient group: 7.7 ± 0.9 vs 12.3 ± 0.6 nmol/mg protein in the control group. Further analysis of the data revealed that patients with multiple defects of the ETC had the most marked GSH deficiency: 4.1 ± 0.9 nmol/mg protein (n = 4, p < 0.05) when compared to the control group. These findings suggest that a deficiency in skeletal muscle GSH concentration is associated with an ETC defect, possibly as a consequence of diminished ATP availability or increased oxidative stress. The decreased ability to combat oxidative stress could therefore cause further loss of ETC activity and hence be a contributing factor in the progressive nature of this group of disorders. Furthermore, restoration of cellular GSH status could prove to be of therapeutic benefit in patients with a GSH deficiency associated with their ETC defects.
On the existence of blowing-up solutions for a mean field equation
Authors: Pistoia Angela , Esposito Pierpaolo , Grossi Massimo 
Published: February 2005

In this paper we construct single and multiple blowing-up solutions to the mean field equation:;

\begin{cases} −\mathrm{\Delta }u = \lambda \frac{V(x)\:\mathrm{e}^{u}}{\int _{\Omega }V(x)\:\mathrm{e}^{u}} & \text{in }\Omega \text{,} \\ u = 0 & \text{on }\partial \Omega \text{,}
\end{cases}

where

Ω

is a smooth bounded domain in

\mathbb{R}^{2}

,

V

is a smooth function positive somewhere in

Ω

and

λ

is a positive parameter.

Résumé
Dans ce papier nous construisons des solutions qui explosent pour l'équation de champ moyen :

\begin{cases}
−\mathrm{\Delta }u = \lambda \frac{V(x)\:\mathrm{e}^{u}}{\int _{\Omega }V(x)e^{u}} & \text{in }\Omega \text{,} \\ u = 0 & \text{on }\partial \Omega \text{,}
\end{cases}

où

Ω

est un domaine borné dans

\mathbb{R}^{2}

,

V

est une fonction positive dans

Ω

et

λ

est un paramètre positif.
Constitutive activity modulation of human metabotropic glutamate 5a receptors in HEK293 cells: a role for key amino‐terminal cysteine residues
Authors: Mühlemann Andreas , Diener Catherine , Fischer Christophe , Piussi Jenny , Stucki Andy , Porter Richard H 
Published: February 2005

Several combinations of cysteine to serine mutations at positions 57, 93, 99 and 129 in the extracellular N‐terminal domain of human metabotropic 5a (hmGlu5a) receptors were produced and expressed in HEK293 cells. Quisqualic acid‐induced intracellular calcium ([Ca2+]i) mobilization and inositol phosphates (IP) accumulation revealed an apparent increased efficacy and decreased potency for hmGlu5a mutants C57S, C99S and C57/99S, as well as a total loss of function for the mutant C57/93/99/129S.
[3H]Quisqualate saturation analysis revealed mutants C57S, C99S, C57/99S and the tetramutant C57/93/99/129S to have unchanged KD but reduced Bmax values. [3H]MPEP saturation analysis on the same membrane preparations revealed no difference in KD for any mutant, but a decrease in Bmax value for the C57/93/99/129S receptor.
Inverse agonism of MPEP at hmGlu5a receptors was partially reduced by mutation C57S, significantly reduced by C99S and C57/99S mutations and totally abolished in the tetramutant.
We confirmed the surface expression of all the mutated receptors using [3H]MPEP binding analysis on whole cells. However, Bmax values were increased for mutant C57S, C99S, and C57/99S but decreased in the C57/93/99/129S receptor.
The 24 h preincubation of cells expressing hmGlu5a receptors with 1 μM MPEP followed by extensive washing dramatically increased the wild‐type receptor efficacy to quisqualate, to the same levels seen with C57/99S receptors. MPEP preincubation did not affect C57/99S function.
We conclude that cysteines 57 and 99 are key residues necessary for modulating hmGlu5a receptor function.

British Journal of Pharmacology (2005) 144, 1118–1125. doi:10.1038/sj.bjp.0706152
Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3)
Authors: SWIERCZ Rafal , PERSON Maria D. , BEDFORD Mark T. 
Published: February 2005

PRMT3 (protein arginine methyltransferase 3) is one of four type I arginine methyltransferases that catalyse the formation of asymmetric dimethylarginine. PRMT3 is unique in that its N-terminus harbours a C2H2 zinc-finger domain that is proposed to confer substrate specificity. In addition, PRMT3 is the only type I enzyme that is restricted to the cytoplasm. Known in vitro substrates for PRMT3 include GST–GAR (a glutathione S-transferase fusion protein containing the glycine- and arginine-rich N-terminal region of fibrillarin), Sam68 (Src-associated substrate during mitosis 68 kDa) and PABP-N1 [poly(A)-binding protein-N1; PABP2]. Here we report the identification of an in vivo substrate for mammalian PRMT3. We found that FLAG-tagged PRMT3 can ‘pull down’ a protein with a molecular mass of 30 kDa from HeLa cell extracts. MS identified this PRMT3-interacting protein as rpS2 (ribosomal protein S2). In vitro studies showed that the zinc-finger domain of PRMT3 is necessary and sufficient for binding to rpS2. In addition, rpS2 is methylated by PRMT3 in vitro and is also methylated in cell lines. Deletion analysis of the rpS2 amino acid sequence identified a N-terminal Arg-Gly repeat as the methylation site. Furthermore, both PRMT3 and rpS2 co-sediment with free ribosomal subunits. These studies implicate PRMT3 in ribosomal function and in the regulation of protein synthesis.
Family average income and body mass index above the healthy weight range among urban and rural residents in regional Mainland China
Authors: Xu Fei , Yin Xiao-Mei , Zhang Min , Leslie Eva , Ware Robert , Owen Neville 
Published: February 2005

AbstractObjectiveTo explore the relationship between family average income (FAI; an index of socio-economic status) and body mass index (BMI; a widely used, inexpensive indicator of weight status) above the healthy weight range in a region of Mainland China.DesignPopulation-based cross-sectional study, conducted between October 1999 and March 2000 on a sample of regular local residents aged 35 years or older who were selected by random cluster sampling.SettingForty-five administrative villages selected from three urban districts and two rural counties of Nanjing municipality, Mainland China, with a regional population of 5.6 million.SubjectsIn total, 29 340 subjects participated; 67.7% from urban and 32.3% from rural areas; 49.8% male and 50.2% female. The response rate among eligible participants was 90.1%.ResultsThe proportion of participants classified as overweight was 30.5%, while 7.8% were identified as obese. After adjusting for possible confounding variables (age, gender, area of residence, educational level, occupational and leisure-time physical activity, daily vegetable consumption and frequency of red meat intake), urban participants were more likely to be overweight or obese relative to their rural counterparts, more women than men were obese, and participants in the lowest FAI tertile were the least likely to be above the healthy weight range.ConclusionsThe proportion of adults with BMI above the healthy weight range was positively related to having a higher socio-economic status (indexed by FAI) in a regional Chinese population.
Associations between parental report of the home food environment and adolescent intakes of fruits, vegetables and dairy foods
Authors: Hanson Nicole I , Neumark-Sztainer Dianne , Eisenberg Marla E , Story Mary , Wall Melanie 
Published: February 2005

AbstractObjectiveThis study examines parental report of household food availability, parent dietary intake and associations with adolescent intakes of fruits, vegetables and dairy foods.DesignCross-sectional study. Adolescents completed the Project EAT survey and the Youth Adolescent Food Frequency Questionnaire at school. Parents of adolescents were interviewed by telephone about the home food environment, eating habits and weight-related behaviours. General linear modelling was used to compare dietary intakes of adolescents across different levels of household food availability and parental intakes.Subjects/settingThe study sample included 902 adolescents and their parent or guardian.ResultsMany parents were not consuming the minimum number of daily recommended fruit (44.5%), vegetable (69.9%) or dairy (46.9%) servings. While most parents reported that fruits and vegetables were available at home (90.3%) and vegetables were usually served at dinner (87.0%), fewer parents reported milk was served at meals (66.6%). Soft drinks were usually available at home (56.8%). Among girls, household availability was positively associated with fruit and vegetable intake (ttrend = 2.70, P < 0.01) and soft drink availability was inversely associated with dairy intake (ttrend = 2.08, P = 0.04). Among boys, serving milk at meals was positively associated with dairy intake (ttrend = 3.65, P < 0.01). Parental intakes were positively associated with dairy intake for boys (ttrend = 2.04, P = 0.04), and with dairy (ttrend = 2.43, P = 0.01), vegetable (ttrend = 3.72, P < 0.01) and fruit (ttrend = 3.17, P < 0.01) intakes for girls.Conclusions/applicationsInterventions designed to help adolescents improve consumption of fruits, vegetables and dairy foods may be enhanced by including a parental component aimed at increasing household availability and parents' intake of healthful food choices.
A regional food-frequency questionnaire for the US Mississippi Delta
Authors: Tucker Katherine L , Maras Janice , Champagne Catherine , Connell Carol , Goolsby Susan , Weber Judith , Zaghloul Sahar , Carithers Teresa , Bogle Margaret L 
Published: February 2005

AbstractObjectiveTo describe food sources of nutrient intake for white and African American adults in the Lower Mississippi Delta (LMD), and their use in the development of a regional food-frequency questionnaire (FFQ) based on an earlier version of the National Cancer Institute's Health Habits and History Questionnaire.DesignWe ranked food sources of energy, macronutrients, vitamins and minerals, and examined portion size distributions for 842 white and 857 African American residents aged 19 years and older, using 24-hour dietary intake recall data from a telephone survey of 36 LMD counties. These values were used to develop a regional FFQ, which was then field-tested with 100 subjects and revised to improve interpretability.SettingThe LMD region of the USA.SubjectsWhite and African American adult residents of the LMD.ResultsLMD African Americans obtained more of their energy and nutrient intakes from poultry, processed meat, salty snacks, fruit drinks, pork and cornbread; and less from milk, alcohol, legumes, salad dressing, butter/margarine and sweetened tea than did white residents. Regional foods not on nationally used FFQs included grits, turnip greens, okra, ham hocks, chitterlings, crawfish, catfish, cracklings, jambalaya, potato logs, chicken and dumplings, and sweet potato pie. Based on responses during field-testing, the questionnaire was also designed to add four portion sizes for each food item, presented as questions, rather than in grid format.ConclusionsRegional food use patterns differ from national patterns and furthermore differ between African American and white adults in the LMD. The resulting Delta NIRI FFQ for Adults should contribute to improved assessment of usual intake for use in studies of diet and health in this region.
Acceptability of multiple micronutrient supplements by pregnant and lactating women in Mali
Authors: Aguayo Víctor M , Koné Diakalia , Bamba Sory Ibrahim , Diallo Baba , Sidibé Yacouba , Traoré Diakalia , Signé Pierre , Baker Shawn K 
Published: February 2005

AbstractBackgroundIn Mali, an estimated 73% of pregnant women are anaemic largely due to iron deficiency. National policy recommends women to take iron and folic acid supplements daily from first prenatal contact until 3 months postpartum. However, many pregnant women in Mali could benefit from multiple micronutrient supplements.ObjectiveTo assess pregnant women's acceptability of and adherence to a daily multiple micronutrient supplementation scheme compared with the current daily iron and folic acid supplementation scheme.DesignSeventy pregnant women were allocated to either the daily multiple micronutrient or daily iron and folic acid supplementation scheme. Women started receiving supplements at the end of the first trimester of pregnancy until delivery and throughout the first 3 months postpartum.ResultsNo significant differences were observed between comparison groups with respect to women's perceptions about supplement size, colour, taste or flavour. Adherence to the multiple micronutrient supplementation scheme was better (257.5±20.9 tablets; average adherence 95.4%) than that to the iron and folic acid supplementation scheme (238.5±32.7 tablets; average adherence 92.2% P = 0.008) although both were very good, as were women's perceptions about the benefits of micronutrient supplements to their health and that of their newborns.ConclusionMalian women adhere to prenatal/postpartum micronutrient supplementation – no matter what supplement is chosen – when access to supplements is guaranteed and when they are provided with minimum, consistent and easily understandable information and counselling, indicating that these are key elements to ensure effective programmes. These findings, together with those of the global research agenda on the efficacy of multiple micronutrient supplements for pregnant women, will inform policy development in Mali for the effective control of iron deficiency and iron-deficiency anaemia in pregnant women.
THE INCIDENCE AND LEVEL OF LISTERIA SPP. AND LISTERIA MONOCYTOGENES CONTAMINATION IN PROCESSED POULTRY AT A POULTRY PROCESSING PLANT
Authors: LOURA CLÁUDIA A.C. , ALMEIDA ROGERIA C.C. , ALMEIDA PAULO F. 
Published: February 2005

ABSTRACT To estimate the incidence and levels of Listeria spp. in an industrial poultry processing plant, samples of chicken breast meat, livers, surfaces of saws and tables, hands and gloves were analyzed. Forty percent of the breast samples presented Listeria: L. monocytogenes, L. innocua and L. grayi. Liver samples were contaminated by L. innocua and L. grayi. High levels of Listeria monocytogenes were found on saws (<30–2400 MPN/equipment) and tables (<30–11,000 MPN/equipment). Hands were contaminated by L. monocytogenes, L. innocua and L. grayi and gloves with L. innocua and L. grayi. The levels of Listeria on hands and gloves were low (<110 MPN/hand). L. monocytogenes serotypes were 1/2a, 1/2b, 1/2c and 4b. Overall, the study demonstrated the high prevalence of Listeria spp. and specifically L. monocytogenes in chicken breast meat, equipment and hands. Improvements and innovations at the poultry processing plant may effectively reduce final production contamination with Listeria.
Expression of the bcl‐6 and MUM1/IRF4 proteins correlate with overall and disease‐specific survival in patients with primary cutaneous large B‐cell lymphoma: a tissue microarray study
Authors: Sundram Uma , Kim Youn , Mraz‐Gernhard Serena , Hoppe Richard , Natkunam Yasodha , Kohler Sabine 
Published: February 2005

Background:  Systemic B‐cell lymphomas have been studied using microarrays, which has led to a better understanding of their molecular characteristics. Initial microarray studies of these lymphomas have implicated several genes as important predictors of outcome. In this study, we used a tissue microarray (TMA) to characterize primary cutaneous large B‐cell lymphomas (PCLBCL).Methods:  We studied 14 patients for whom clinical follow up was available, including four patients whose lesions were limited to the leg on presentation. Immunohistochemical staining with CD20, CD44, CD21, CD5, CD10, bcl‐2, bcl‐6, Ki67, p53, and multiple myeloma 1 (MUM1) was examined.Results:  Our results identify two subgroups of lymphomas. The first group showed staining with bcl‐6 and had an overall survival of 176 months (p = 0.003). The majority of this group was negative for MUM1. The second group lacked staining with bcl‐6 and had an overall survival of 26 months, with a majority of these cases staining with MUM1. Three of four patients with PCLBCL of the leg showed no staining with bcl‐6.Conclusions:  Our study demonstrates the utility of TMAs in the analysis of PCLBCL and that expression of bcl‐6 and MUM1 correlates with survival.