bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Nuclear Shuttle Protein ofTomato Leaf Curl New Delhi VirusIs a Pathogenicity Determinant
Authors: Hussain Mazhar , Mansoor Shahid , Iram Shazia , Fatima Ayesha Naureen , Zafar Yusuf 
Published: March 2005

ABSTRACTThe role of the movement protein (MP) and nuclear shuttle protein (NSP) in the pathogenicity ofTomato leaf curl New Delhi virus(ToLCNDV), a bipartite begomovirus, was studied. Both genes were expressed inNicotiana benthamiana,Nicotiana tabacum, andLycopersicon esculentumplants with thePotato virus X(PVX) expression vector or by stable transformation of gene constructs under the control of the 35S promoter inN. tabacum. No phenotypic changes were observed in any of the three species when the MP was expressed from the PVX vector or constitutively expressed in transgenic plants. Expression of the ToLCNDV NSP from the PVX vector inN. benthamianaresulted in leaf curling that is typical of the disease symptoms caused by ToLCNDV in this species. Expression of NSP from PVX inN. tabacumandL. esculentumresulted in a hypersensitive response (HR), demonstrating that the ToLCVDV NSP is a target of host defense responses in these hosts. The NSP, when expressed as a transgene under the control of the 35S promoter, resulted in necrotic lesions in expanded leaves that initiated from a point and then spread across the leaf. The necrotic response was systemic in all the transgenic plants. Deletion of 100 amino acids from the C terminus did not compromise the HR response, suggesting that this region has no role in HR. Deletion of 60 or 100 amino acids from the N terminus of NSP abolished the HR response, suggesting that these sequences are required for the HR response. These findings demonstrate that the ToLCNDV NSP is a pathogenicity determinant as well as a target of host defense responses.
Mumps Virus V Protein Antagonizes Interferon without the Complete Degradation of STAT1
Authors: Kubota Toru , Yokosawa Noriko , Yokota Shin-ichi , Fujii Nobuhiro , Tashiro Masato , Kato Atsushi 
Published: March 2005

ABSTRACTMumps virus (MuV) has been shown to antagonize the antiviral effects of interferon (IFN) through proteasome-mediated complete degradation of STAT1 by using the viral V protein (T. Kubota et al., Biochem. Biophys. Res. Commun. 283:255-259, 2001). However, we found that MuV could inhibit IFN signaling and the generation of a subsequent antiviral state long before the complete degradation of cellular STAT1 in infected cells. In MuV-infected cells, nuclear translocation and phosphorylation of STAT1 and STAT2 tyrosine residue (Y) at 701 and 689, respectively, by IFN-β were significantly inhibited but the phosphorylation of Jak1 and Tyk2 was not inhibited. The transiently expressed MuV V protein also inhibited IFN-β-induced Y701-STAT1 and Y689-STAT2 phosphorylation, suggesting that the V protein could block IFN-β-induced signal transduction without the aid of other viral components. Finally, a substitution of an alanine residue in place of a cysteine residue in the C-terminal V-unique region known to be required for STAT1 degradation and inhibition of anti-IFN signaling resulted in the loss of V protein function to inhibit the Y701-STAT1 and Y689-STAT2 phosphorylation.
The Alpha/Beta Interferon Response Controls Tissue Tropism and Pathogenicity of Poliovirus
Authors: Ida-Hosonuma Miki , Iwasaki Takuya , Yoshikawa Tomoki , Nagata Noriyo , Sato Yuko , Sata Tetsutaro , Yoneyama Mitsutoshi , Fujita Takashi , Taya Choji , Yonekawa Hiromichi , Koike Satoshi 
Published: March 2005

ABSTRACTPoliovirus selectively replicates in neurons in the spinal cord and brainstem, although poliovirus receptor (PVR) expression is observed in both the target and nontarget tissues in humans and transgenic mice expressing human PVR (PVR-transgenic mice). We assessed the role of alpha/beta interferon (IFN) in determining tissue tropism by comparing the pathogenesis of the virulent Mahoney strain in PVR-transgenic mice and PVR-transgenic mice deficient in the alpha/beta IFN receptor gene (PVR-transgenic/Ifnarknockout mice). PVR-transgenic/Ifnarknockout mice showed increased susceptibility to poliovirus. After intravenous inoculation, severe lesions positive for the poliovirus antigen were detected in the liver, spleen, and pancreas in addition to the central nervous system. These results suggest that the alpha/beta IFN system plays an important role in determining tissue tropism by protecting nontarget tissues that are potentially susceptible to infection. We subsequently examined the expression of IFN and IFN-stimulated genes (ISGs) in the PVR-transgenic mice. In the nontarget tissues, ISGs were expressed even in the noninfected state, and the expression level increased soon after poliovirus infection. On the contrary, in the target tissues, ISG expression was low in the noninfected state and sufficient response after poliovirus infection was not observed. The results suggest that the unequal IFN response is one of the important determinants for the differential susceptibility of tissues to poliovirus. We consider that poliovirus replication was observed in the nontarget tissues of PVR-transgenic/Ifnarknockout mice because the IFN response was null in all tissues.
The Epstein-Barr Virus EBNA-LP Protein Preferentially Coactivates EBNA2-Mediated Stimulation of Latent Membrane Proteins Expressed from the Viral Divergent Promoter
Authors: Peng RongSheng , Moses Stephanie C. , Tan Jie , Kremmer Elisabeth , Ling Paul D. 
Published: March 2005

ABSTRACT
The mechanistic contribution of the Epstein-Barr virus (EBV) EBNA-LP protein to B-cell immortalization remains an enigma. However, previous studies have indicated that EBNA-LP may contribute to immortalization by enhancing EBNA2-mediated transcriptional activation of the LMP-1 gene. To gain further insight into the potential role EBNA-LP has in EBV-mediated B-cell immortalization, we asked whether it is a global or gene-specific coactivator of EBNA2 and whether coactivation requires interaction between these proteins. In type I Burkitt's lymphoma cells, we found that EBNA-LP strongly coactivated EBNA2 stimulation of LMP-1 and LMP2B RNAs, which are expressed from the viral divergent promoter. Surprisingly, the viral LMP2A gene and cellular CD21 and Hes-1 genes were induced by EBNA2 but showed no further induction after EBNA-LP coexpression. We also found that EBNA-LP did not stably interact with EBNA2 in coimmunoprecipitation assays, even though the conditions were adequate to observe specific interactions between EBNA2 and its cellular cofactor, CBF1. Colocalization between EBNA2 and EBNA-LP was not detectable in EBV-transformed cell lines or transfected type I Burkitt's cells. Finally, no significant interactions between EBNA2 and EBNA-LP were found with mammalian two-hybrid assays. From this data, we conclude that EBNA-LP is not a global coactivator of EBNA2 targets, but it preferentially coactivates EBNA2 stimulation of the viral divergent promoter. While this may require specific transient interactions between these proteins that only occur in the context of the divergent promoter, our data strongly suggest that EBNA-LP also cooperates with EBNA2 through mechanisms that do not require direct or indirect complex formation between these proteins.
Dual Role of the Lymphocytic Choriomeningitis Virus Intergenic Region in Transcription Termination and Virus Propagation
Authors: Pinschewer Daniel D. , Perez Mar , de la Torre Juan Carlos 
Published: March 2005

ABSTRACT
Each genome segment of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV), encodes two genes in ambisense orientation, separated by an intergenic region (IGR). The 3′ ends of subgenomic viral mRNAs have been mapped to a stem-loop structure within the IGR, suggesting structure-dependent transcription termination. We have studied the role of the LCMV IGR by using a minigenome (MG) rescue system based on RNA analogues of the short genome segment. An ambisense MG coding for chloramphenicol acetyltransferase (CAT) and green fluorescent protein reporter genes instead of the nucleoprotein and glycoprotein open reading frames, respectively, served as a template for synthesis of full-length anti-MG (aMG) replicate and subgenomic size mRNA for reporter gene expression. An analogous MG without IGR was amplified by the virus polymerase with equal efficiency, but subgenomic mRNA was undetectable. Reporter gene expression from IGR-deficient aMG CAT-sense RNA of genomic length was approximately 5-fold less efficient than that from subgenomic CAT mRNA derived from an IGR-containing MG, but at least 100-fold more efficient than that from a T7 RNA polymerase transcript with the same sequence. Therefore, in the absence of IGR-mediated transcription termination, a fraction of full-length aMG RNA appears to behave as bona fide mRNA. Unexpectedly, MGs without IGR were dramatically impaired in their ability to passage reporter gene activity via infectious virus-like particles. These data suggest that the LCMV IGR serves individual functions in transcription termination for enhanced gene expression and in the virus assembly and/or budding, which are required for the efficient propagation of LCMV infectivity.
Role of the Right and Left Hemispheres in Recovery of Function during Treatment of Intention in Aphasia
Authors: Crosson Bruce , Moore Anna Bacon , Gopinath Kaundinya , White Keith D. , Wierenga Christina E. , Gaiefsky Megan E. , Fabrizio Katherine S. , Peck Kyung K. , Soltysik David , Milsted Christina , Briggs Richard W. , Conway Tim W. , Rothi Leslie J. Gonzalez 
Published: March 2005

Abstract
Two patients with residual nonfluent aphasia after ischemic stroke received an intention treatment that was designed to shift intention and language production mechanisms from the frontal lobe of the damaged left hemisphere to the right frontal lobe. Consistent with experimental hypotheses, the first patient showed improvement on the intention treatment but not on a similar attention treatment. In addition, in keeping with experimental hypotheses, the patient showed a shift of activity to right presupplementary motor area and the right lateral frontal lobe from pre-to post-intention treatment functional magnetic resonance imaging (fMRI) of language production. In contrast, the second patient showed improvement on both the intention and attention treatments. During pre-treatment fMRI, she already showed lateralization of intention and language production mechanisms to the right hemisphere that continued into post-intention treatment imaging. From pre-to post-treatment fMRI of language production, both patients demonstrated increased activity in the posterior perisylvian cortex, although this activity was lateralized to left-hemisphere language areas in the second but not the first patient. The fact that the first patient's lesion encompassed almost all of the dominant basal ganglia and thalamus whereas the second patient's lesion spared these structures suggests that the dominant basal ganglia could play a role in spontaneous reorganization of language production functions to the right hemisphere. Implications regarding the theoretical framework for the intention treatment are discussed.
Behavioral and Electrophysiological Evidence of a Right Hemisphere Bias for the Influence of Negative Emotion on Higher Cognition
Authors: Simon-Thomas Emiliana R. , Role Kemi O. , Knight Robert T. 
Published: March 2005

Abstract
We examined how responses to aversive pictures affected performance and stimulus-locked event-related potentials (ERPs) recorded during a demanding cognitive task. Numeric Stroop stimuli were briefly presented to either left or right visual hemifield (LVF and RVF, respectively) after a centrally presented aversive or neutral picture from the International Affective Picture System. Subjects indicated whether a quantity value from each Stroop stimulus matched the preceding Stroop stimulus while passively viewing the pictures. After aversive pictures, responses were more accurate for LVF Stroops and less accurate for RVF Stroops. Early-latency extrastriate attention-dependent visual ERPs were enhanced for LVF Stroops. The N2 ERP was enhanced for LVF Stroops over the right frontal and parietal scalp sites. Slow potentials (300–800 msec) recorded over the frontal and parietal regions showed enhanced picture related modulation and amplitude for LVF Stroops. These results suggest that emotional responses to aversive pictures selectively facilitated right hemisphere processing during higher cognitive task performance.
Expectation and the Vestibular Control of Balance
Authors: Guerraz Michel , Day Brian L. 
Published: March 2005

AbstractRecent experiments have shown that the visual channel of balance control is susceptible to cognitive influence. When a subject is aware that an upcoming visual disturbance is likely to arise from an external agent, that is, movement of the visual environment, rather than from self-motion, the whole-body response is suppressed. Here we ask whether this is a principle that generalizes to the vestibular channel of balance control. We studied the whole-body response to a pure vestibular perturbation produced by galvanic vestibular stimulation (GVS; 0.5 mA for 3 sec). In the first experiment, subjects stood with vision occluded while stimuli were delivered either by the subject himself (self-triggered) or by the experimenter. For the latter, the stimulus was delivered either without warning (unpredictable) or at a fixed interval following an auditory cue (predictable). Results showed that GVS evoked a whole-body response that was not affected by whether the stimulus was self-triggered, predictable, or unpredictable. The same results were obtained in a second experiment in which subjects had access to visual information during vestibular stimulation. We conclude that the vestibular-evoked balance response is automatic and immune to knowledge of the source of the perturbation and its timing. We suggest the reason for this difference between visual and vestibular channels stems from a difference in their natural abilities to signal self-motion. The vestibular system responds to acceleration of the head in space and therefore always signals self-motion. Visual flow, on the other hand, is ambiguous in that it signals object motion and eye motion, as well as self-motion.
The Randomized Sample Tree: A Data Structure for Interactive Walk-Throughs in Externally Stored Virtual Environments
Authors: Klein Jan , Krokowski Jens , Fischer Matthias , Wand Michael , Wanka Rolf , Heide Friedhelm Meyer auf der 
Published: March 2005

We present a new data structure for rendering highly complex virtual environments of arbitrary topology. The special feature of our approach is that it allows an interactive navigation in very large scenes (30 GB/400 million polygons in our benchmark scenes) that cannot be stored in main memory, but only on a local or remote hard disk. Furthermore, it allows interactive rendering of substantially more complex scenes by instantiating objects. The sampling process is done in the preprocessing. There, the polygons are randomly distributed in our hierarchical data structure, the randomized sample tree. This tree uses only space that is linear in the number of polygons. In order to produce an approximate image of the scene, the tree is traversed and polygons stored in the visited nodes are rendered. During the interactive walk-through, parts of the sample tree are loaded from a local or remote hard disk. We implement our algorithm in a prototypical walk-through system. Analysis and experiments show that the quality of our images is comparable to images computed by the conventional z-buffer algorithm regardless of the scene topology.
Predicting red muscle performance
Authors: Curtin  , Lou  , Woledge  
Published: March 2005

Abstract
The active force production during contraction of bundles of red muscle fibres from myotomal muscle of dogfish Scyliorhinus canicula (L.) has been predicted by a numerical model based on Hill's 2-component model of the mechanical properties of muscle, with the addition of a simple model for the kinetics of activation. When all of these factors are included, the model makes good predictions for the force and power during stimulation. There are, however, significant discrepancies during relaxation after the end of stimulation, from which we conclude that additional processes have a large influence on force during relaxation. This has functional relevance for fish swimming because during relaxation the muscle fibre continues its sinusoidal pattern of movement and thus continues to either produce power if shortening, or require power input from other structures to cause lengthening.
Alien Species Stay Home: The International Convention for the Control and Management of Ships' Ballast Water and Sediments 2004
Authors: Tsimplis Michael 
Published: March 2005

AbstractThe transfer of organisms by ships has been altering the ecosystems for many decades. Living organisms within ballast water is part of this problem. Where ballast water has been taken from heavily polluted areas then potential risks for human health are also created. The International Convention for the Control and Management of Ships' Ballast Water (the Ballast Water Convention—referred to hereafter as the BWC) is the first international attempt to provide a legal and technical instrument for a risk that was not so far covered by any legal regime. The BWC imposes obligations both on the flag and the port states and provides for a certification system that will eventually be able to regulate ballast water management. Ambitious ballast water quality standards are included but they will not be imposed until 2016 when a new generation of ships having the required new technology will be developed. These strict standards are subject to review on the basis of their feasibility and cost implications. In the meantime only some basic precautions and restrictions, together with a complex surveying and certification system, will be imposed. Moreover, these restrictions will only be imposed if they do not cause delay or deviation for the ships, arguably making one wonder whether the problem is indeed a serious and imminent threat to the environment.
Rim 2/Hipa CACTA transposon display ; A new genetic marker technique in Oryza species
Authors: Kwon Soon-Jae , Park Kyong-Chul , Kim Jin-Hong , Lee Ju Kyong , Kim Nam-Soo 
Published: March 2005

Abstract
Background
Transposons constitute the major fractions of repetitive sequences in eukaryotes, and have been crucial in the shaping of current genomes. Transposons are generally divided into two classes according to the mechanism underlying their transposition: RNA intermediate class 1 and DNA intermediate class 2. CACTA is a class 2 transposon superfamily, which is found exclusively in plants. As some transposons, including the CACTA superfamily, are highly abundant in plant species, and their nucleotide sequences are highly conserved within a family, they can be utilized as genetic markers, using a slightly modified version of the conventional AFLP protocol. Rim2 /Hipa is a CACTA transposon family having 16 bp consensus TIR sequences to be present in high copy numbers in rice genome. This research was carried out in order to develop a Rim2/Hipa CACTA-AFLP or Rim2/Hipa CACTA-TD (transposon display, hereafter Rim2/Hipa-TD) protocol for the study of genetic markers in map construction and the study of genetic diversity in rice.

Results
Rim2/Hipa-TD generated ample polymorphic profiles among the different rice accessions, and the amplification profiles were highly reproducible between different thermocyclers and Taq polymerases. These amplification profiles allowed for clear distinction between two different ecotypes, Japonica and Indica, of Oryza sativa. In the analysis of RIL populations, the Rim2/Hipa-TD markers were found to be segregated largely in a dominant manner, although in a few cases, non-parental bands were observed in the segregating populations. Upon linkage analysis, the Rim2/Hipa-TD markers were found to be distributed in the regions proximal to the centromeres of the chromosomes. The distribution of the Rim2/Hipa CACTA elements was surveyed in 15 different Oryza species via Rim2/Hipa-TD. While Rim2/Hipa-TD yielded ample amplification profiles between 100 to 700 bp in the AA diploid Oryza species, other species having BB, CC, EE, BBCC and CCDD, profiles demonstrated that most of the amplified fragments were larger than 400 bp, and that our methods were insufficient to clearly distinguish between these fragments. However, the overall amplification profiles between species in the Oryza genus were fully distinct. Phenetic relationships among the AA diploid Oryza species, as evidenced by the Rim2/Hipa-TD markers, were matched with their geographical distributions.

Conclusion
The abundance of the Rim2/Hipa TIR sequences is very informative since the Rim2/Hipa-TD produced high polymorphic profiles with ample reproducibility within a species as well as between species in the Oryza genus. Therefore, Rim2/Hipa-TD markers can be useful in the development of high-density of genetic map around the centromeric regions. Rim2/Hipa-TD may also prove useful in evaluations of genetic variation and species relationships in the Oryza species.
Recognition and binding of mismatch repair proteins at an oncogenic hot spot
Authors: Edelbrock Michael , He Huiling , Schroering Allen , Fernstrom Martha , Bathala Sangeetha , Williams Kandace J 
Published: March 2005

Abstract
Background
The current investigation was undertaken to determine key steps differentiating G:T and G:A repair at the H-ras oncogenic hot spot within the nuclear environment because of the large difference in repair efficiency of these two mismatches.

Results
Electrophoretic mobility shift (gel shift) experiments demonstrate that DNA containing mismatched bases are recognized and bound equally efficiently by hMutSα in both MMR proficient and MMR deficient (hMLH1-/-) nuclear extracts. Competition experiments demonstrate that while hMutSα predictably binds the G:T mismatch to a much greater extent than G:A, hMutSα demonstrates a surprisingly equal ratio of competitive inhibition for both G:T and G:A mismatch binding reactions at the H-ras hot spot of mutation. Further, mismatch repair assays reveal almost 2-fold higher efficiency of overall G:A repair (5'-nick directed correct MMR to G:C and incorrect repair to T:A), as compared to G:T overall repair. Conversely, correct MMR of G:T → G:C is significantly higher (96%) than that of G:A → G:C (60%).

Conclusion
Combined, these results suggest that initiation of correct MMR requires the contribution of two separate steps; initial recognition by hMutSα followed by subsequent binding. The 'avidity' of the binding step determines the extent of MMR pathway activation, or the activation of a different cellular pathway. Thus, initial recognition by hMutSα in combination with subsequent decreased binding to the G:A mismatch (as compared to G:T) may contribute to the observed increased frequency of incorrect repair of G:A, resulting in the predominant GG C → GT C (Gly → Val) ras- activating mutation found in a high percentage of human tumors.