bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional Analysis of SAA3 During Interaction Between Actinobacillus pleuropneumoniaeand Porcine Alveola Macrophages
Authors: Bei Weicheng , He Qiyun , yan Kang , Tang Jia , Gu Jun , Zheng Yaxuan , Dou Beibei , Yang Fengming , Peng Mingzheng , Tian Yanhong , Chen Huanchun 
Published: November 2023

Abstract
The spread of Porcine contagious pleuropneumonia (PCP), a severe disease that occurs in pigs caused by Actinobacillus pleuropneumoniae (APP), remains a threat to the porcine farms and has been known to cause severe economic losses. Serum amyloid A (SAA) is an acute-phase protein rapidly expressed in response to infection and inflammation in vertebrates. This study aimed to investigate the function of SAA3 in bacterial infections. Here, APP was used to infect porcine alveolar macrophages (3D4/21) and mice. The results showed that SAA3 expression was significantly upregulated in 3D4/21 cells and APP-infected mouse lung tissue. The expression of IL-1β, IL-6, and TNF-α were also upregulated in SAA3-overexpressing (SAA3-OE) cells. APP levels were substantially upregulated in the supernatant of SAA3-OE cells. Also, SAA3-OE promoted the adsorption and invasion of APP-infected target cells. These results suggested that porcine SAA3 promoted the proliferation of APP, providing insights into the role of porcine SAA3 in the course of bacterial infection.
Refining and Illuminating Acetogenic Eubacterium Strains for Reclassification and Metabolic Engineering
Authors: Flaiz Maximilian , Poehlein Anja , Wilhelm Wiebke , Mook Alexander , Daniel Rolf , Dürre Peter , Bengelsdorf Frank R. 
Published: November 2023

Abstract
Background: The genus Eubacterium is quite diverse and includes several acetogenic strains capable of fermenting C1-substrates into valuable products. Especially, Eubacterium limosum and closely related strains attract attention not only for their capability to ferment C1 gases and liquids, but also due to their ability to produce butyrate. Apart from its well-elucidated metabolism, E. limosum is also genetically accessible, which makes it an interesting candidate to be an industrial biocatalyst.
Results: In this study, we examined genomic, phylogenetic, and physiologic features of E. limosum and the closest related species E. callanderi as well as E. maltosivorans. We sequenced the genomes of the six Eubacterium strains ‘FD’ (DSM 3662T), ‘Marburg’ (DSM 3468), ‘2A’ (DSM 2593), ‘11A’ (DSM 2594), ‘G14’ (DSM 107592), and ‘32’ (DSM 20517) and subsequently compared these with previously available genomes of the E. limosum type strain (DSM 20543T) as well as the strains ‘B2’, ‘KIST612’, ‘YI’ (DSM 105863T), and ‘SA11’. This comparison revealed a close relationship between all eleven Eubacterium strains, forming three distinct clades: E. limosum, E. callanderi, and E. maltosivorans. Moreover, we identified the gene clusters responsible for methanol utilization as well as genes mediating chain elongation in all analyzed strains. Subsequent growth experiments revealed that strains of all three clades can convert methanol and produce acetate, butyrate, and hexanoate via reverse β-oxidation. Additionally, we used a harmonized electroporation protocol and successfully transformed eight of these Eubacterium strains to enable recombinant plasmid-based expression of the gene encoding the fluorescence-activating and absorption shifting tag (FAST). Engineered Eubacterium strains were verified regarding their FAST-mediated fluorescence at a single-cell level using a flow cytometry approach. Eventually, strains ‘FD’ (DSM 3662T), ‘2A’ (DSM 2593), ‘11A’ (DSM 2594), and ‘32’ (DSM 20517) were genetically engineered for the first time.
Conclusion: Strains of E. limosum, E. callanderi, and E. maltosivorans are outstanding candidates as biocatalysts for anaerobic C1-substrate conversion into valuable biocommodities. A large variety of strains is genetically accessible using a harmonized electroporation protocol, and FAST can serve as a reliable fluorescent reporter protein to characterize genetically engineered cells. In total eleven strains have been assigned to distinct clades, providing a clear and updated classification. Thus, the description of respective Eubacterium species has been emended, improved, aligned, and is requested to be implemented in respective databases.
Pyogenic Granuloma of the Inferior Turbinate :A Case Report
Authors: Pari Vishnupriya , Arya Ajay Kumar , Thirunavukarasu Yuvaraj 
Published: November 2023

Abstract
BACKGROUND
Pyogenic granuloma is a rapidly growing benign fibrovascular lesion involving the skin and mucous membranes. It usually affects the head and neck regions. The most common site is the oral cavity, while involvement of the nasal cavity is quite rare. Though few cases in the nasal cavity have been reported, occurrence in the inferior turbinates is extremely rare. The exact aetiology is unknown.It is often associated with pregnancy. Microtrauma, hormonal changes, and contraceptives may be the causative factors for the development of this vascular mass.
CASE PRESENTATION
Here we present the case of a 28-year-old woman, 2 weeks postpartum, with epistaxis and nasal obstruction. Diagnostic nasal endoscopy revealed a vascular mass arising from the right inferior turbinate. The patient was treated successfully by endoscopic excision of the mass.Histopatholoical examination showed as pyogenic granuloma.
CONCLUSION
Pyogenic granuloma should be considered as a differential diagnosis in any pregnant woman presenting with a vascular mass in the head and neck region.
Acute Venous Thromboembolism Plasma and Red Blood Cell Metabolomic Profiling Reveals Potential New Early Diagnostic Biomarkers: observational clinical study
Authors: Febra Claudia , Saraiva Joana , Vaz Fátima , Macedo Joao , Al-Hroub Hamza Mohammad , Semreen Mohammad H , Maio Rui , Gil Vitor , Soares Nelson , Penque Deborah 
Published: November 2023

Abstract
Background:Venous thromboembolism(VTE) is a leading cause of cardiovascular mortality. The diagnosis of acute VTE is still based on complex imaging exams due to the lack of biomarkers. Moreover, studies assessing the diagnostic capacity of novel metabolomics biomarkers in VTE are scarce. Our aim was to determine whether patients with acute VTE have differences in the metabolomic profile from plasma and red blood cells (RBCs).
Methods: This observational trial included 62 patients with clinical suspicion of acute deep vein thrombosis (DVT) or pulmonary embolism (PE) admitted to the emergency room (ER). After gold standard imaging exams, we analysed the plasma and RBCs from 50 acute VTE and 12 nonacute VTE patients. We performed a metabolomics study and used mixed-effects modelling to compare the differences in metabolites.
Results:The plasma metabolome had a suboptimal capability for differentiating between the presence or absence of acute VTE, with 23 significantly different molecules, but with ‘good’ performance for the best ROC curves. The metabolic pathway of D-glutamine and D-glutamate had the strongest impact on the acute VTE phenotype (p = 0.001, false discovery rate = 0.06). RBCs revealed a consistent metabolomic signature of acute VTE. Among the 23 differentially abundant metabolites, we found 3 high-performance ROC curves with an area under the curve (AUC) higher than 0.9, including adenosine 3',5'-diphosphate (0.983), glutathione (0.923), and adenine (0.91). The metabolic set most impacting the differences observed was purine metabolism (p = 0.000354, false discovery rate = 0.68).
Conclusions:Our findings show that metabolite differences exist between acute VTE and nonacute VTE patients admitted to the ER in the early phases. Three potential biomarkers obtained from RBCs showed high performance for acute VTE diagnosis. Further studies should investigate accessible laboratory methods for the future daily practice usefulness of these metabolites for the early diagnosis of acute VTE in the ER.
Epidemiology, management and diagnostic workup for endometriosis related pelvic pain
Authors: Warzecha Damian , Salloum Daria , Wielgoś Mirosław , Sochacki-Wójcicka Nicole , Laudański Piotr 
Published: November 2023

Abstract
Background: It’s believed that around 10% of gynecology appointments are related to pelvic pain. Although in most cases the underlying etiology remains unknown, endometriosis is one of the most causes of this condition. The aim of our study was to investigate the epidemiology, diagnostic workup and management of dysmenorrhea, dyspareunia and chronic pelvic pain among women suffering from endometriosis.
Methods: An electronic, anonymous questionnaire was prepared by the authors and shared by social media. Women were asked to identify the bases of the diagnosis of their endometriosis in accordance with the European Society of Human Reproduction and Embryology (ESHRE) guidelines. The study protocol included only pre-menopausal women, aged 16–56 years. 33,769 respondents took part in the survey. The credibility of completed surveys was checked to exclude the possibility of bias arising from malicious bots. The study group included 2,862 females with the diagnosis of endometriosis, according to the ESHRE guidelines.
Results: The prevalence of endometriosis within the overall Polish population can be estimated at 8.7%. The mean time elapsed between the first symptoms and final confirmation of the disease was 11.8 years. The incidence and severity of all analyzed complaints was significantly higher that among the patients without previous confirmation of endometriosis (dysmenorrhea 84% vs 65.1%; dyspareunia 52.9% vs 25.5%; chronic pelvic pain 61.8% vs 25.4%) Patients suffering from endometriosis were older than the unaffected controls (33.3 vs 31.6 years, p <0.0001) and of lower BMI levels (24.0 vs 24.6, p = <0.0001) Endometriotic patients report painful symptoms to their gynecologist almost twice as often as unaffected controls suffering from any kind of pelvic pain (88.9% vs 48.7%. p < 0.0001). 27.7% of patients suffering from endometriosis experience a non-satisfactory reduction in dysmenorrhea and pelvic pain after painkillers.
Conclusion: Although endometriotic patients report painful symptoms to the gynecologist almost twice as often as unaffected women, the diagnostic delay is still unacceptably long. Due to high rate of non-satisfactory reduction in dysmenorrhea and pelvic pain after taking painkillers simultaneous treatment with other options such as hormonal therapy should be introduced as early as possible.
Circulating miR-451a, miR-5571-3p, CLN6, HSD11B1 and PDE4 levels in differential diagnosis of Granulomatous lobular mastitis, breast cancer and breast fibroadenoma
Authors: LING JIE , WU SHITING , DENG XIANGUANG , ZHOU YAO , SU JINGQUN , FAN HONGQIAO , LIU LIFANG 
Published: November 2023

Abstract
This study aimed to investigate the diagnostic value of miR-451a, miR-5571-3p, CLN6, HSD11B1, and PDE4 as potential biomarkers for distinguishing patients with Granulomatous lobular mastitis (GLM), breast cancer (BC), and breast fibroadenoma. A total of 55 participants were included in each group. The serum expressions of miR-451a and miR-5571-3p were significantly higher in the GLM (0.95 ± 0.47, 7.20 ± 3.99) and control groups (0.21 ± 0.13, 1.74 ± 1.49) than in the BC group (p < 0.01). Meanwhile, the expression of miR-451a and miR-5571-3p in GLM was significantly higher than in the control group (0.21 ± 0.13, 1.74 ± 1.49; p < 0.001). The levels of CLN6 in the GLM(1.37 ± 0.87) and BC((1.57 ± 0.84) groups were higher than those in the control group (p < 0.001). Furthermore, HSD11B1 and PDE4 expressions were significantly increased in the BC (1.92 ± 1.14, 1.54 ± 0.88) and GLM (1.07 ± 0.70, 0.85 ± 0.51) groups compared to the control group (p < 0.01), with higher levels observed in the BC group than in the GLM group (p < 0.01). Taken together, these findings suggest that serum miR-451a, miR-5571-3p, and CLN6 may serve as noninvasive biomarkers for differentiating GLM, BC, and breast fibroadenoma. This study provides a basis for future investigations in the differential diagnosis of these breast diseases.
Does a Higher Sense of Gain to Primary Health Care Institutions Lead to a Greater Frequency of First Contact?
Authors: Lin Jianpeng , Lv Wenxin , Li Qiong , Zhang Linjie , Cao Xianqiang 
Published: November 2023

Abstract

Background
China hopes to reach the policy goal of patients' first contact with primary health care (PHC) institutions through several health care reform policies. Sense of gain is an essential yardstick for measuring reform proposed in China in recent years. However, how high are patients' "sense of gain" (SG) and frequency of first contact with PHC institutions in China, and what is the relationship between the two? These questions still need findings from relevant empirical studies.
Methods
An empirical study was conducted using descriptive statistics, correlation analysis, and robust ordered logistic regression methods based on objective statistics from 50 randomly sampled cities in mainland China in 2018 and subjective survey data on access to PHC institutions in 2020.
Results
In this study, we found that patients' sense of gain to PHC institutions in China in 2020 was medium, and the frequency of patients' first contact with PHC institutions "frequently" and above was low. Different aspects of patients' sense of gain to PHC institutions had a differential impact on the frequency of first contact to PHC institutions. There is a significant positive effect on patients' sense of quantity, quality, equity, and improvement of PHC, but no significant effect on accessibility. Patients' sense of gain to PHC institutions has a differential effect on the frequency of first contact with PHC institutions in different ranks of cities. Sense of quantity has a significant effect only in higher-rank cities. In contrast, the sense of quality and improvement has a significant effect only in lower-rank cities.
Conclusion
It is necessary for the government to scientifically consider the development status of cities of different ranks and pay attention to the actual demand and expectation of patients for PHC. Then effectively improve patients' sense of gain to achieve the policy goal of patients' first contact with PHC institutions.
Biosynthesized copper oxide nanoparticles using aloe vera leaves extract and their evaluation of antibacterial, anticancer in human Hela cancer cells
Authors: Rafique Saima , Akram Rizwan , Javed Tooba , Bashir Shazia , Noreen Zobia 
Published: November 2023

Abstract
This research work assessed the shape-dependent anti-cancer activity of copper oxide nanoparticles (CuO NPs) synthesized from aloe vera leaf extract on the human HeLa cell line (ATCCCCL-2TM) and their antimicrobial action against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The CuO NPs were synthesized by varying the concentration of precursor and aging time to obtain different shapes of nanoparticles. The synthesized particles had rod-like, cube-shaped, triangular, and spherical morphologies. The CuO NPs were examined against gram-positive and gram-negative bacteria. The results showed that NPs inhibit the growth of these bacteria and antibacterial activity depends on the size and shape of CuO NPs. The minimum inhibition concentration obtained was 0.325 mgml− 1 for E. coli at 0.2 M and 24 hrs aging time. A dose-dependent reduction in cell viability was observed using CuO NPs. CuO NPs caused significant morphological alterations in Hela cell lines, including shrinkage, detachment, and distorted shape. These findings imply that different-shaped CuO NPs may inhibit bacterial growth, elevate oxidative stress, and induce apoptosis in addition shows cytotoxic effects on cancer cells.
Criteria for Evaluating Faculty Members Based on Expectations of Beneficiaries in Tabriz University of Medical Sciences: A qualitative study
Authors: Azizi Hanieh , Alizadeh Mahasti , Gholipour Kamal , Abadi Saeed Aslan , Azadifar Ali , Behshid Mozhgan 
Published: November 2023

Abstract
Objective:
This study aimed to identify the evaluation criteria used for faculty members’ evaluation, as viewed from the perspective of various stakeholders at Tabriz University of Medical Sciences.
Methods:
The present study is a qualitative investigation that was carried out at Tabriz University of Medical Sciences. The study employed 74 semi-structured interviews in addition to a focused group discussion that involved students, graduates, faculty members, as well as experts. Participants for this research were chosen using the Purposive Sampling technique, and the data were evaluated through content analysis methodology. The interviews were recorded with participants' informed consent and transcribed verbatim. Subsequent analysis of all data started with the first interview session.
Results:
Participants had expectations about the evaluation of faculty members categorized into scientific and technical features and moral and behavioral characteristics. The scientific and technical features included Scientific and practical capability, Scientific records, and Teaching methods, while the moral and behavioral features consisted of Professional ethics and behavior, Communication skills, and Ethics and Behavior.
Conclusion:
Based on the results, it is essential to adopt systemic approaches in evaluating professors that would consider various technical, human, and perceptual aspects. Management of the evaluation process considering environmental and structural features is also necessary to improve its effectiveness.
Using machine learning to develop a five-item short form of the Children’s Depression Inventory
Authors: Lin Shumei , Wang Chengwei , Jiang Xiuyu , Zhang Qian , Luo Dan , Li Jing , Li Junyi , Xu Jiajun 
Published: November 2023

Abstract

Background
There are many adolescents experience depression that is not detected and treated in time. The Children’s Depression Inventory (CDI) is widely used in China, but no localized revision or simplified form has been formulated. Consequently, the present study aimed to formulate an abbreviated version of the CDI with only five items, using a large sample of 20,675 Chinese children aged 7 to 15 years in Sichuan Province.
Methods
First, different versions of the short-form scales were identified by backward elimination. Then, the area under the ROC curve (AUC) of five machine learning (ML) algorithms on the short-form scales were compared. Finally, the prediction performance of each short-form scale was evaluated with the metric of the naïve Bayes (NB).
Results
The study identified a five-item short-form CDI with a judgment threshold of 4 as the most appropriate scale considering all assessment indicators. The scale had 81.48% fewer items than the original version, indicating good predictive performance (AUC = 0.81, Accuracy = 0.83, Recall = 0.76, Precision = 0.71). Based on the test of 315 middle school students, the results showed that the five-item CDI had good measurement indexes (Cronbach’s alpha = 0.72, criterion-related validity = 0.77).
Conclusions
This five-item short-form CDI is the first shortened and revised version of the CDI in China based on large local data samples.
NatB-dependent acetylation protects procaspase-8 from UBR4-mediated degradation and is required for full induction of the extrinsic apoptosis pathway
Authors: Aldabe Rafael , Guedes Joana , Boyer Jean , Elurbide Jasmin , Carte Beatriz , Redeker Virginie , Sago Laila , Meinnel Thierry , Côrte-Real Manuela , Giglione Carmela 
Published: November 2023

Abstract
N-terminal acetyltransferase B (NatB) is a major contributor to the N-terminal acetylome and is implicated in several key cellular processes including apoptosis and proteostasis. However, the molecular mechanisms linking NatB-mediated N-terminal acetylation to apoptosis and its relationship with protein homeostasis remain elusive. In this study, we generated mouse embryonic fibroblasts (MEFs) with an inactivated catalytic subunit of NatB (Naa20−/−) to investigate the impact of NatB deficiency on apoptosis regulation. Through quantitative N-terminomics, label-free quantification, and targeted proteomics, we demonstrated that NatB does not influence the proteostasis of all its substrates. Instead, our focus on putative NatB-dependent apoptotic factors revealed that NatB-mediated acetylation serves as a protective shield against UBR4 and UBR1 Arg/N-recognin-mediated degradation. Notably, Naa20−/− MEFs exhibited reduced responsiveness to extrinsic pro-apoptotic stimuli, a phenotype that was partially reversible upon UBR4 Arg/N-recognin silencing and consequent inhibition of procaspase-8 degradation. Collectively, our results shed light on how the interplay between NatB-mediated acetylation and the Arg/N-degron pathway impacts apoptosis regulation, providing new perspectives in the field including in therapeutic interventions.
A prospective study of immune responses in patients with lung metastases treated with stereotactic body radiotherapy with or without concurrent systemic treatment
Authors: Duda Dan , Gkika Eleni , Firat Elke , Adebahr Sonja , Graf Erika , Eichhorst Alexandra , Radicioni Gianluca , Lo Simon , Spohn Simon , Nestle Ursula , Nicolay Nils , Niedermann Gabriele , Grosu Anca-Ligia 
Published: November 2023

Abstract
We prospectively evaluated the effects of stereotactic body radiotherapy (SBRT) on circulating immune cells. Patients with oligo-metastatic and oligo-progressive pulmonary lesions were treated with SBRT with (cSBRT) or without (SBRT group) concurrent systemic treatment (chemotherapy or immune checkpoint blockade) using different fractionation regimes. Immunoprofiling of peripheral blood cells was performed at baseline, during, at the end of SBRT, and at the first and second follow-ups. The study accrued 100 patients (80 with evaluable samples). The proportion of proliferating CD8+ T-cells significantly increased after treatment. This increase remained significant at follow-up in the SBRT group, but not in the cSBRT group and was not detected with doses of >10Gy per fraction indicating that lower doses are necessary to increase proliferating T-cells' frequency. We detected no favorable impact of concurrent systemic treatment on systemic immune responses. The optimal timing of systemic treatment may be post-SBRT to leverage the immune-modulating effects of SBRT.
Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan
Authors: AKIMITSU Kazuya , Poti Teeranai , Kisaki Gan , Akimitsu Kazuya 
Published: November 2023

Abstract
Anthracnose caused by Colletotrichum species is one of the serious diseases of loquat, but diversification of the species of Colletotrichum associated with loquat in Japan has not been adequately surveyed. In this study, 46 isolates were isolated from diseased leaves and fruits of loquat in Kagawa and Tokushima prefectures in 2017 and 2018. Using a combination of morphological features and molecular approaches, seven Colletotrichum species were identified: C. fioriniae, C. nymphaeae, C. karsti, C. aenigma, C. fructicola, C. gloeosporioides s. s., and C. siamense. The most prevalent species was C. karsti followed by C. fioriniae and C. gloeosporioides s. s., accounting for 43.5, 21.7, and 13.0%, respectively. All identified species were confirmed to be the causal agents of loquat anthracnose by applying Koch’s postulates. Moreover, potential cross-infection from loquat to kiwifruit, satsuma mandarin, persimmon, and peach was found, in which loquat isolates of C. fioriniae, C. fructicola, and C. siamense caused symptoms on all the tested plants. This report is the first on C. karsti and C. aenigma associated with loquat anthracnose and the first record of C. fructicola and C. gloeosporioides s. s. in Japan, providing crucial information for epidemiology.