bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time is a Funny Thing: Response Times and Humor Quality in a Creative Joke Production Task
Authors: Silvia Paul J. , Cotter Katherine N. , Christensen Alexander P. 
Published: February 2024

Abstract
Generating creative ideas takes time: the first idea to come to mind is usually obvious, and people need time to shift strategies, enact executive processes, and evaluate and revise an idea. The present research explored the role of time in creative humor production tasks, which give people a prompt and ask them to create a funny response. A sample of 152 young adults completed four joke stems prompts. Their response times were recorded, and the responses were judged for humor quality (funniness) by six independent judges and by the participants themselves. Mixed-effect models found that, at the within-person level, response time’s link to humor quality diverged for judges and participants. The judges’ ratings of funniness predicted longer response times (relatively funnier responses took longer to create), but participants’ self-ratings of their own responses predicted shorter response times (relatively funnier responses were created faster). Controlling for elaboration (quantified via word count of the response) diminished the effect of judge-rated humor but not participant-rated humor. Taken together, the results suggest that the role of time in humor generation is complex: judges may be weighting elaboration more heavily when judging funniness, whereas participants may be weighting metacognitive cues like ease-of-generation when judging their own ideas.
Human activity recognition with fine-tuned CNN-LSTM
Authors: Genc Erdal , Yildirim Mustafa Eren , Salman Yucel Batu 
Published: February 2024

Abstract
Human activity recognition (HAR) by deep learning is a challenging and interesting topic. Although there are robust models, there is also a bunch of parameters and variables, which affect the performance such as the number of layers, pooling type. This study presents a new deep learning architecture that is obtained by fine-tuning of the conventional CNN-LSTM model, namely, CNN (+3)-LSTM. Three changes are made to the conventional model to increase the accuracy. Firstly, kernel size is set to 1×1 to extract more information. Secondly, three convolutional layers are added to the model. Lastly, average pooling is used instead of max-pooling. Performance analysis of the proposed model is conducted on the KTH dataset and implemented on Keras. In addition to the overall accuracy of the proposed model, the contribution of each change is observed individually. Results show that adding layers made the highest contribution followed by kernel size and pooling, respectively. The proposed model is compared with state-of-art and outperformed some of the recent studies with a 94.1% recognition rate.
Design and implementation of a current-fed dual active bridge converter for an AC battery
Authors: Do Tuan Anh , Nguyen Quang Dich , Vu Phuong 
Published: February 2024

Abstract
Home battery has become more and more popular in which the AC battery is a trending product along with the AC-coupled system. The energy conversion of an AC battery commonly consists of a two-stage power converter to flexibly connect to the wide-range input battery voltage and different grid or load types. This study specifically concentrates on the DC/DC stage which plays an important role in transferring power with the battery. In addressing the need for isolation, bidirectional power flow, and limited battery current ripple, the Current-fed Dual Active Bridge (CFDAB) structure is chosen for this stage. This paper presents a comprehensive design for the CFDAB converter for an AC battery application, especially a technical solution focusing on zero voltage switching is applied to increase the efficiency of the converter. Finally, an experimental prototype is carried out to validate the performance of the CFDAB converter in both two modes of the power flow.
On Darbo’s fixed point principle
Authors: Taoudi Mohamed Aziz 
Published: February 2024

Abstract
In this paper, we prove the following generalization of the classical Darbo fixed point principle : Let X be a Banach space and µ be a montone measure of noncompactness on X which satisfies the generalized Cantor intersection property. Let C be a nonempty bounded closed convex subset of X and T : C → C be a continuous mapping such that for any countable set Ω ⊂ C, we have µ(T(Ω)) ≤ kµ(Ω), where k is a constant, 0 ≤ k < 1. Then T has at least one fixed point in C. The proof is based on a combined use of topological methods and partial ordering techniques and relies on the Schauder and the Knaster-Tarski fixed point principles.
Basins of attraction of a one-parameter family of root-finding techniques
Authors: Basto Mário , Basto Mário Alberto 
Published: February 2024

Abstract
Initial conditions can have a substantial impact on the behavior of iterative root-finding techniques for nonlinear equations. By allowing complex starting points and complex roots, it is possible to examine the basins of attraction in the complex plane in order to compare the performance of various iterative techniques. In this paper, a one-parameter family of third-order root-finding methods is studied by varying its parameter A within −2.0 and 2.4 and applying it to a polynomial equation of high degree (degree 25). This family includes the Euler–Chebyshev’s (A = 0), Halley’s (A = 1) and BSC (A = 2) techniques. According to the results, the one-parameter family provides the best performance for values near A = 1, which equals to the Halley’s method.
On some generalization of order cancellation law for subsets of topological vector space
Authors: Przybycie Hubert 
Published: February 2024

Abstract
In this paper we generalize a result of R. Urbański from paper [7] which states that for subsets A, B, C of topological vector space X the following implication holds

A
+
B
⊂
B
+
C
⇒
A
⊂
C

A + B \subset B + C \Rightarrow A \subset C

provided that B is bounded and C is closed and convex. The generalization is given in Theorem 5 where we prove this result for k- convex subsets of a topological vector space. Also we introduce a notion of some abstract like-closure operation for subsets of linear space and we study its connections to order cancellation law.
Supercritical CO2 Extraction of Fish Roe
Authors: Luksta Ilze , Mika Taras , Spalvins Kriss 
Published: February 2024

Abstract
Seafood is rich in omega-3 polyunsaturated fatty acids, and the good emulsifying properties of phospholipids make them suitable for use in the food, pharmaceutical and cosmetic industries. This study attempted to evaluate different fish species obtained using supercritical carbon dioxide without and with and ethanol as co-solvent extraction and organic solvent (ethanol) extraction. Supercritical CO2 extraction was performed at 30–50 °C and at different pressures (17.7–33.0 MPa), maintaining a CO2 flow rate of 0.6 mL/min for 3 hours. Gas chromatography and Bligh & Dyer methods were used to analyze the extracts. The results showed that only one species of fish roe (rainbow trout) produced oil, but it was concluded that improvements were needed to be able to extract oil from African catfish roe using supercritical CO2 extraction. Rainbow trout roe contains the most monounsaturated fatty acids (44 %).
Assessing the Sustainability Impact of Improving Secondary Steel Production: Lessons Learned from an Italian Plant
Authors: Tomasoni Giuseppe , Marciano Filippo , Stefana Elena , Cocca Paola 
Published: February 2024

Abstract
This work presents a sustainability assessment approach to evaluate technological innovations in secondary steel production making use of Electric Arc Furnace (EAF) technology. The assessment covers the environmental, social, and economic dimensions of sustainability by combining different tools (Life Cycle Assessment and Analytic Hierarchy Process), and also provides an integrated assessment of the overall sustainability. The approach, which can also be used to support decision-making, has been applied to a real case study of a steel plant located in Northern Italy. In the case study, environmental sustainability is positively impacted mainly by increased metal yield and reduced furnace energy consumption. The greatest social sustainability benefits are mainly related to improved ergonomic and safety conditions for workers (reduced demand for physical effort, manual handling and repetitiveness, and lower risk of accidents), as a consequence of the introduction of Industry 4.0 technologies. Regarding economic sustainability, a positive impact related to reduced cycle time, increased metal yield and quality yield, reduced maintenance and quality control costs was observed. The integrated assessment of the overall sustainability has proven to be a viable approach to manage trade-offs between the different dimensions of sustainability.
Factors Affecting the Recession of Construction Brick Industry: A Case of Moklan Brick Kiln Factory Nakhon Si Thammarat, Thailand
Authors: Prommuang Phawat , Seviset Somchai , Egwutvongsa Songwut 
Published: February 2024

Purpose of the Study: Construction brick industry has generated high income and it is the main revenue of Moklan city residents for a long time. However, recently, there has been a huge decrease of demand due to several reasons both from internal and external factors. The output of this research can be used to solve the problem and revive Moklan city economy. The purpose are 1) To study factors affecting the recession of the construction brick manufacturing industry in Nakhon Si Thammarat Province. 2) To propose a guideline for solving the recession problems.

Methodology: This is qualitative research using in-depth interview as a research tool. The data were collected from 40 owners of the Moklan brick kiln factory in Nakhon Si Thammarat, Thailand selected by purposive method. Data were analyzed by content and thematic analysis.

Main Findings: 1) Factors affecting to the recession of the industry consist of technology changes, environmental concerns, domestic economy, and product quality. 2) The guideline for solving this recession, the product must be environmentally friendly products, correspond to personal demand of customers, durable, and comprehensive usage.

Applications: The output of this research can be applied in construction brick companies to improve their competitive advantage and production design and process.

Novelty/Originality: This study reveal the factors to revive construction brick companies in Nakhon Si Thammarat , Thailand.
Pengaruh Starter Ragi, EM4 Dan Variasi Rasio C/N Terhadap Hasil Biogas Kulit Kakao
Authors: Ridwan Iskandar , Yani Endri , Utami Lega Putri , Maulana Ifza 
Published: February 2024

Cocoa is an essential ingredient for making chocolate. Still, the only part of the cocoa used to produce chocolate is the cocoa beans, while around 90% of the total weight of the cocoa is immediately discarded in the form of cocoa shells. This causes cocoa shells to become plentiful, whereas many are processed into animal feed and compost. In compost processing, a decomposition process occurs, which potentially produces biogas. Biogas production from cocoa shells can use fermentation tools such as digesters. This research method is carried out through several stages. First, cocoa shells are collected from cocoa production originating from the Lima Puluh Kota district of West Sumatra. Then, the cocoa shells are prepared and processed into raw materials for biogas production. The fermentation process is carried out using yeast starter and EM4 for 21 days of observation, which is a mixture of microorganisms. The research variation consisted of adding 150 g of yeast and increasing the volume of EM4 in each digester except the control digester. The addition of EM4 for the C/N ratio test digesters 25, 27, 29 is 0.124, 0.099, 0.083 liters. The total gas production obtained from each digester is 9.054 liters (DU29), 2.590 liters (DU27), 2.562 liters (DU25), and 1.307 liters (DK). Meanwhile, the levels of methane gas produced in each test sample are 8.77% (DK), 10.47% (DU25), 18.02% (DU27), and 21.43% (DU29). The color of the gas flame produced by the DU29 and DU27 is blue, the DU25 is red, and the DK is reddish yellow.
Studi Performa Cooling Tower berdasarkan Kualitas Air Pendingin
Authors: Yani Endri , Putra Akmal Hadi Yurihuda 
Published: February 2024

Cooling water is used to cool fluids with a high temperature in a closed system, such as a cooling tower or heat exchanger. Sometimes, the cooling water is specified in a long tube, which risks fouling and rusk. This research will study cooling water quality as Total Dissolved Solid (TDS), chlorine concentration, and antiscale. In the design of the cooling tower at PLTGU Tanjung Uncang, the range or limit of the permitted TDS is 50,000. If this figure has been reached, the cooling tower must be blown down. Blowdown is water in the cooling tower that is deliberately removed to avoid the concentration of impurities during heat evaporation. At PLTGU Tanjung Uncang, the permitted chlorine concentration is in the range of 0.2-0.5. Chlorine flow in the cooling tower at PLTGU Tanjung Uncang is reversed with Cl concentration and TDS. Inject Antiscale aims to maintain cooling tower performance, which aims to be anti-rust.
Pelatihan Pengembangan Game Digital Dalam Mendorong Pertumbuhan Industri Kreatif
Authors: Kurniawan Haris , Mulyono Heri , Samudra Ami Anggraini 
Published: February 2024

Pelaksanaan Kurikulum Merdeka yang masih baru khususnya di Sekolah Menengah Kejuruan (SMK) jurusan Rekayasa Perangkat Lunak (RPL) ataupun Multimedia dimana adanya pelajaran pengembangan game yang guru-gurunya belum terlalu menguasai materi dan prakteknya. Hal ini akan berdampak kepada keterampilan siswa dalam pembelajaran pengembangan game. Seiring dengan kompetensi itu tentunya juga perlu mempersiapkan lulusan SMK untuk dapat terjun kedunia kerja ataupun menjadi wirausaha di industri kreatif game ini. Saat ini pemerintah juga mendorong generasi muda dan pelaku usaha kreatif untuk menumbuh kembangkan kompetensi ini sebagai salah satu penyumbang pertumbuhan industri game atau ekonomi kreatif di Indonesia. Dengan permasalahan tersebut, maka perlu dilakukan upaya peningkatan keterampilan dan pemahaman dalam bentuk pelatihan di bidang pengembangan dan pemrograman game sekaligus juga pelatihan menjadi wirausaha game digital. Metode dalam pelatihan ini menggunakan model Goad. Pelatihan dilakukan dengan 5 langkah dengan peserta siswa jurusan RPL dan Mulmedia SMK IT Al-Hidayah Pasaman Barat. Hasil dari kegiatan ini adalah kemampuan membuat game digital dan juga pemahaman tentang wirausaha digital. Jadi dengan selesainya pelatihan ini peserta sudah bisa membuat atau mengembangkan game digital serta mampu menjadi wirausaha game tentunya sebagai penyumbang pertumbuhan industri kreatif di Indonesia khususnya bagi lulusan SMK.
Creation of A549 and MCF7 tumor sublines with knockout of TP53 using CRISPR/Cas9
Authors: Хамидуллина А.И. , Гандалипов Э.Р. , Абраменко Я.Е. , Чернов К.В. , Кирюхина Т.А. , Брутер А.В. , Татарский В.В. 
Published: February 2024

Опухолевой супрессор p53 является центральным звеном защиты клетки от злокачественной трансформации. Мутации кодирующего гена TP53 наблюдаются приблизительно в половине опухолей человека и способствуют не только опухолевой прогрессии, но также устойчивости или чувствительности к противоопухолевым препаратам. Получение изогенных линий, имеющих разный статус TP53, необходимо не только для исследования его роли, но и для скрининга новых противоопухолевых препаратов и их комбинаций. Получение изогенных моделей с помощью системы CRISPR/Cas9 часто связано с одноклеточным клонированием, что приводит к клональным эффектам из-за гетерогенности опухолевых культур. В данной работе описано получение новых нокаутов TP53 в линиях MCF7 и A549 с помощью системы CRISPR/Cas9 и отбора по устойчивости к нутлину-3, позволяющей отбирать нокаутные клетки без этапа клонального отбора. Фенотипически нокаут был подтвержден по полному отсутствию белка p53, снижению экспрессии p53-зависимого гена CDKN1A и изменению чувствительности к ДНК-повреждающим противоопухолевым препаратам.
The tumor suppressor p53 is the central point of cellular defense against oncogenic transformation. Mutations of TP53 gene are present in approximately half of human tumors and promote not only tumor progression, but also resistance to anticancer drugs. Creation of isogenic models, differing in their TP53 status, is valuable not only for studying its role in carcinogenesis, but also for screening of anticancer drugs and their combinations. Establishment of isogenic models using the CRISPR/Cas9 system is usually done through single cell cloning, which can lead to clonal effects, because of heterogeneity of the cell cultures. In this article we present the process of creation of new knockout cell sublines of MCF7 and A549 using CRISPR/Cas9 and selection using nutlin-3, which allows selecting cell sublines without clonal selection. Phenotypically the knockout of TP53 was confirmed by total absence of p53, absence of induction of p53-dependent gene CDKN1A, and shift in sensitivity to DNA-damaging drugs.
Ethical aspects of whole-genome noninvasive prenatal testing
Authors: Потапов А.А. , Капланова М.Т. , Галактионова А.М. , Баранова Е.Е. , Сагайдак О.В. , Ижевская В.Л. , Денисов А.Г. 
Published: February 2024

Неинвазивный пренатальный тест (НИПТ) – современный молекулярно-генетический метод, применяемый для скрининга хромосомной патологии у плода путем выделения и секвенирования свободно циркулирующих фрагментов ДНК плода и плаценты в крови матери (внеклеточная фетоплацентарная ДНК). НИПТ стал активно применяться в клинической практике сравнительно недавно – с начала прошлого десятилетия, в связи с чем нет единого мнения и решений этических вопросов, связанных с тестированием. Селекция пола, формирование спектра выявляемых рисков, медико-генетическое консультирование, дискриминация детей с хромосомными аномалиями и их родителей, «рутинизация» метода – основные этические проблемы, с которыми сталкиваются врачи различных специальностей и пациенты. Влияние результата НИПТ на репродуктивный выбор семейных пар сложно недооценить, поэтому применение теста в системе пренатального скрининга требует особого внимания, консультирования и участия специалистов разных областей медицины и не только.
Non-invasive prenatal testing (NIPT) is a molecular genetic method of assessing the risks of fetal chromosomal abnormalities from cell-free fetal DNA by isolating and sequencing cell-free fetal and placental DNA fragments in maternal blood (cell-free fetoplacental DNA). NIPT has been in use for the past decade, so as a consequence, there is still no consensus or solution regarding the ethical issues surrounding the testing. Gender selection, the range of risks assessed, genetic counseling, discrimination against children with chromosomal abnormalities and their parents, and the «routinization» of the method are the main ethical problems physicians of different specialties and patients face. The impact of NIPT results on the reproductive choices of couples is hard to underestimate, so the involvement of various medical and other specialists in the system of prenatal screening requires particular consultative attention and assistance.
Genome editing by CRISPR/Cas to generate A549 human lung cancer subline knockout for the p21 gene
Authors: Персиянцева Н.А. , Казанский Д.Б. , Татарский В.В. , Замкова М.А. 
Published: February 2024

Технология CRISPR/Cas, являющаяся наиболее эффективной среди существующих методов редактирования генома, позволяет модифицировать таргетные участки молекулы ДНК и находит применение в различных отраслях биологии, генетики, сельского хозяйства, биотехнологии и медицины. С ее помощью создаются модели клеточных линий, необходимые для исследования роли определенных генов в развитии и терапии злокачественных и других заболеваний. Одним из таких генов является р21 (CDKN1A), регулируемый опухолевым супрессором р53 и негативно контролирующий прогрессию клеточного цикла. Неоднозначное влияние белка р21 на процессы канцерогенеза и ключевое участие в ответе клеток на терапию определяют его как перспективную мишень для исследований в этих областях. В нашей работе мы получили сублинию клеток рака легкого А549, нокаутную по гену р21, с помощью метода CRISPR/Cas, Ее использование планируется в экспериментах по изучению роли белка р21, а также опосредуемых им механизмов влияния других генов, в ответе клеток на терапию онкологических заболеваний, в частности, становлении фенотипа старения при применении низких доз терапии и выхода отдельных клеток из этой стадии (формирование рецидива опухоли), а также определении механизмов возникновения устойчивости опухолевых клеток.
The CRISPR/Cas technology, which is the most efficient among the existing genome editing methods, allows modifying target regions of the DNA molecule and is used in various fields of biology, genetics, agriculture, biotechnology, and medicine. Models of cell lines required for studying the role of certain genes in the development and therapy of malignant and other diseases can be created using CRISPR/Cas method. One of such genes is p21 (CDKN1A), which is regulated by the p53 tumor suppressor and negatively controls cell cycle progression. The ambiguous influence of the p21 protein on the processes of carcinogenesis and its key role in the response of cells to therapy make it a promising target for research in these areas. In our work, we obtained a subline of lung cancer cells A549, knockout for the p21 gene, using the CRISPR/Cas method. It is planned to use it in experiments to study the role of the p21 protein, as well as the mechanisms mediated by it, the influence of other genes, in the cell response to cancer therapy. in particular, the formation of the senescence phenotype after treatment with low-dose therapy and the escape of individual cells from this stage (formation of tumor recurrence), as well as determining the mechanisms for the emergence of tumor cell resistance.
Clinical significance of PIK3CA mutations in breast cancer subtypes
Authors: Королева Е.И. , Назаров В.Д. , Лапин С.В. , Владыкина А.В. , Анисимов С.В. , Щелканова И.Ю. , Эмануэль В.Л. 
Published: February 2024

Ген PIK3CA входит в число трех наиболее часто мутирующих онкогенов в структуре различных злокачественных новообразований: его дефекты обнаруживаются в образцах рака молочной железы, эндометрия, мочевого пузыря и других видах опухолей. В настоящий момент наибольшую практическую значимость имеет выявление мутаций у пациентов с гормонположительным HER2-отрицательным раком молочной железы, что обусловлено возможностью применения ингибиторов PI3K в терапии данного заболевания. При других молекулярных подтипах воздействие на мутации гена может позволить преодолеть резистентность и увеличить эффективность стандартного лечения. В данной статье изложено клиническое значение выявления мутаций при гормонположительном, HER2-положительном и трижды негативном раке молочной железы, их влияние на прогноз и тактику ведения пациентов, а также описаны проблемы молекулярно-генетического исследования структуры гена. Кроме того, в статье приведены результаты анализа распространенности конкретных мутаций по данным молекулярно-генетического тестирования 118 пациентов, проведенного в лаборатории ПСПбГМУ имени академика И.П. Павлова.
PIK3CA is one of the three most frequently mutated oncogenes in human cancers. The highest rate of mutations is found in breast cancer, endometrial cancer, bladder cancer and some other tumors. Due to integration of PI3K inhibitors in clinical practice, PIK3CA testing is necessary for developing treatment strategies for HR-positive HER2-negative patients. However, PIK3CA testing may play an important role in understanding and treatment of HER2-positive and triple-negative subtypes as well. We hereby discuss clinical significance of PIK3CA mutations in different molecular breast cancer subtypes and possible genetic testing challenges. The article also presents a frequency analysis of different PIK3CA mutations in a study of 118 patients.