bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smoking status among cancer patients by medical specialty: A U.S. nationwide representative analysis
Authors: Nolazco José Ignacio , Tang Yuzhe , Alkhatib Khalid Y. , King Andrew J. , Mossanen Matthew , Chang Steven Lee 
Published: November 2023

AbstractBackgroundPersistence in tobacco use among cancer survivors has been associated with a multitude of clinicodemographic factors. However, there is a paucity of understanding regarding the role the healthcare professional's specialty plays in tobacco cessation in tobacco‐related cancer survivors.MethodsWe conducted a cross‐sectional analysis of data from cancer survivors with a smoking history using the Behavioral Risk Factor Surveillance System (BRFSS) database to examine differences in the proportion of patients continuing tobacco use among patients with a diagnosis of cancer segregated by cancer site specialty over the 2016–2020 period. We accounted for complex survey design and used sampling weights to obtain a nationwide representative sample. We employed modified Poisson regression adjusting for age, gender, education, income, race, marital status, and medical specialty.ResultsWe analyzed 19,855 cancer survivors with a current or past history of tobacco use, of whom 5222 (26,3%) self‐reported to be current smokers. Patients with urological and gynecological tobacco‐related malignancies had a higher relative risk (RR) of being current smokers with a RR of 1.30 (95% confidence interval, 1.12–1.51) and 1.25 (95% confidence interval, 1.12–1.39) respectively. Malignant Hematology had the lowest RR of smoking status among all other specialties RR 0.85 (95% confidence interval, 0.59–1.21).ConclusionsContinuing smoking rates among tobacco‐related cancer survivors were different between specialties. One in four cancer survivors were current smokers; this emphasizes health professionals' paramount role in tobacco cessation counseling.
Gene dosage compensation: Origins, criteria to identify compensated genes, and mechanisms including sensor loops as an emerging systems‐level property in cancer
Authors: Bravo‐Estupiñan Diana M. , Aguilar‐Guerrero Karol , Quirós Steve , Acón Man‐Sai , Marín‐Müller Christian , Ibáñez‐Hernández Miguel , Mora‐Rodríguez Rodrigo A. 
Published: November 2023

AbstractThe gene dosage compensation hypothesis presents a mechanism through which the expression of certain genes is modulated to compensate for differences in the dose of genes when additional chromosomes are present. It is one of the means through which cancer cells actively cope with the potential damaging effects of aneuploidy, a hallmark of most cancers. Dosage compensation arises through several processes, including downregulation or overexpression of specific genes and the relocation of dosage‐sensitive genes. In cancer, a majority of compensated genes are generally thought to be regulated at the translational or post‐translational level, and include the basic components of a compensation loop, including sensors of gene dosage and modulators of gene expression. Post‐translational regulation is mostly undertaken by a general degradation or aggregation of remaining protein subunits of macromolecular complexes. An increasingly important role has also been observed for transcriptional level regulation. This article reviews the process of targeted gene dosage compensation in cancer and other biological conditions, along with the mechanisms by which cells regulate specific genes to restore cellular homeostasis. These mechanisms represent potential targets for the inhibition of dosage compensation of specific genes in aneuploid cancers. This article critically examines the process of targeted gene dosage compensation in cancer and other biological contexts, alongside the criteria for identifying genes subject to dosage compensation and the intricate mechanisms by which cells orchestrate the regulation of specific genes to reinstate cellular homeostasis. Ultimately, our aim is to gain a comprehensive understanding of the intricate nature of a systems‐level property. This property hinges upon the kinetic parameters of regulatory motifs, which we have termed “gene dosage sensor loops.” These loops have the potential to operate at both the transcriptional and translational levels, thus emerging as promising candidates for the inhibition of dosage compensation in specific genes. Additionally, they represent novel and highly specific therapeutic targets in the context of aneuploid cancer.
Comparing the impact of atezolizumab plus bevacizumab and lenvatinib on the liver function in hepatocellular carcinoma patients: A mixed‐effects regression model approach
Authors: Hatanaka Takeshi , Kakizaki Satoru , Hiraoka Atsushi , Tada Toshifumi , Hirooka Masashi , Kariyama Kazuya , Tani Joji , Atsukawa Masanori , Takaguchi Koichi , Itobayashi Ei , Fukunishi Shinya , Tsuji Kunihiko , Ishikawa Toru , Tajiri Kazuto , Ochi Hironori , Yasuda Satoshi , Toyoda Hidenori , Ogawa Chikara , Yokohama Keisuke , Nishikawa Hiroki , Nishimura Takashi , Shimada Noritomo , Kawata Kazuhito , Kosaka Hisashi , Naganuma Atsushi , Yata Yutaka , Ohama Hideko , Kuroda Hidekatsu , Tanaka Kazunari , Tanaka Takaaki , Tada Fujimasa , Nouso Kazuhiro , Morishita Asahiro , Tsutsui Akemi , Nagano Takuya , Itokawa Norio , Okubo Tomomi , Arai Taeang , Imai Michitaka , Koizumi Yohei , Nakamura Shinichiro , Kaibori Masaki , Iijima Hiroko , Hiasa Yoichi , Kudo Masatoshi , Kumada Takashi ,   the Real‐life Practice Experts for HCC (RELPEC) Study Group, and HCC 48 Group (hepatocellular carcinoma experts from 48 clinics in Japan)
Published: November 2023

AbstractAimThis retrospective study compared the impact of atezolizumab plus bevacizumab (Atez/Bev) and lenvatinib (LEN) on the liver function in patients with hepatocellular carcinoma.MethodsWe included 526 patients who received Atez/Bev and 731 who received LEN March 2018 and July 2022 in this study. We conducted a 1:1 propensity‐score‐matched analysis and identified 324 patients in each group for inclusion in the present analysis. Nonlinear mixed‐effects regression models were employed, allowing for the evaluation and inclusion of cases where treatment was interrupted due to disease progression, adverse events, or loss to follow‐up. These models were used to compare the ALBI score between the Atez/Bev and LEN groups.ResultsFollowing propensity score matching, the mean ALBI scores in the Atez/Bev and LEN groups were −2.41 ± 0.40 and −2.44 ± 0.42 at baseline, and −2.17 ± 0.56 and −2.19 ± 0.58 at 12 weeks, respectively. Although the ALBI score significantly worsened during treatment in both groups (p < 0.001), there was no significant difference in the rate of ALBI score deterioration between the groups (p = 0.06). Subgroup analyses showed that LEN‐treated patients with BCLC advanced stage (p = 0.02) and those who initially received the full dose (p < 0.001) had a significantly greater worsening of ALBI score compared to Atez/Bev.ConclusionsUsing a nonlinear mixed‐effects regression approach, which allowed for the inclusion of cases with treatment interruption, we found no significant difference in the trend of liver function deterioration between the Atez/Bev and LEN groups. Caution should be exercised for LEN‐treated patients with BCLC advanced stage or those receiving the full dose of LEN.
New Dipterocarpol‐Based Molecules with α‐Glucosidase Inhibitory and Hypoglycemic Activity
Authors: Smirnova Irina E. , Galimova Zarema I. , Sapozhnikova Tatyana A. , Khisamutdinova Regina Yu. , Thi Thu Ha Nguyen , Kazakova Oxana B. 
Published: November 2023

AbstractDammarane triterpenoids are affordable and bioactive natural metabolites with great structural potential, which makes them attractive sources for drug development. The aim of the study was to investigate the potency of new dipterocarpol derivatives for the treatment of diabetes. Two dammaranes (dipterocarpol and its 20(24)‐diene derivative) were modified by a Claisen‐Schmidt aldol condensation to afford C2(E)‐arylidenes in good yields. The majority of the synthesized compounds exhibited an excellent‐to‐moderate inhibitory effect toward α‐glucosidase (from S. saccharomyces), among them eight compounds showed IC50 values less than 10 μM. 3‐Oxo‐dammarane‐2(E)‐benzylidenes (holding p‐hydroxy‐ 3 l and p‐carbonyl‐ 3 m substituents) demonstrated the most potent α‐glucosidase inhibition with IC50 0.753 and 0.204 μM, being 232‐ and 857‐times more active than acarbose (IC50 174.90 μM), and a high level of NO inhibition in Raw 264.7 cells with IC50 of 1.75 and 4.57 μM, respectively. An in vivo testing of compound 3 m (in a dose of 20 mg/kg) on a model of streptozotocin‐induced T1DM in rats showed a pronounced hypoglycemic activity, the ability to reduce effectively the processes of lipid peroxidation in liver tissue and decrease the excretion of glucose and pyruvic acid in the urine. Compound 3 m reduced the death of diabetic rats and preserved their motor activity.
Electrochemical N‐Aroylation of Sulfoximines by Using Benzoyl Hydrazines with H2 Generation
Authors: Alam Tipu , Patel Bhisma K. 
Published: November 2023

AbstractDeveloped here is a robust electrochemical cross‐coupling reaction between aroyl hydrazine and NH‐sulfoximine via concomitant cleavage and formation of C(sp2)−N bonds with the evolution of H2 and N2 as innocuous by‐products. This sustainable protocol avoids the use of toxic reagents and occurs at room temperature. The reaction proceeds via the generation of an aroyl and a sulfoximidoyl radical via anodic oxidation under constant current electrolysis (CCE), affording N‐aroylated sulfoximine. The strategy is applied to late‐stage sulfoximidation of L‐menthol, (−)‐borneol, D‐glucose, vitamin‐E derivatives, and marketed drugs such as probenecid, ibuprofen, flurbiprofen, ciprofibrate, and sulindac. In addition, the present methodology is mild, high functional group tolerance with broad substrate scope and scalable.
Exclusion of Women from Phase I Trials: Perspectives from Investigators and Research Oversight Officials
Authors: Waltz Margaret , Lyerly Anne Drapkin , Fisher Jill A. 
Published: November 2023

ABSTRACTOver the past 30 years, progress has been made in increasing women's representation in clinical research. However, women continue to be underrepresented in phase I clinical trials—those trials that test the safety and tolerability of investigational drugs, often on healthy individuals. As sex‐based differences in adverse drug reactions are often linked to drug dose, pivotal safety information in phase I trials is often insufficiently—and inequitably—captured for females. Yet there has been little attention to how clinical investigators and those charged with overseeing the ethical conduct of these trials perceive the barriers to women's inclusion in phase I trials. To address this gap, we report on 22 interviews with U.S. phase I investigators and institutional review board (IRB) members. Our findings indicate that although these investigators and IRB members acknowledged the importance of including women in clinical trials, they justified women's exclusion from phase I trials by citing the need to manage their reproductive potential. In particular, we identified four key themes that informants used to warrant women's exclusion from phase I trials: the structure of the drug‐development system itself, fears about risks to potential fetuses, distrust of women to prevent pregnancy, and concerns about risks and burdens to institutions from resulting pregnancies. We argue that these rationales reflect structural and cultural barriers to women's inclusion in clinical research that ultimately fail to respect female research participants as persons, highlighting the need for broad‐based solutions.
Homogeneity in terrestrial land cover is reflected in fish diversity patterns in a Chinese river system
Authors: Kirschner Dominik , Zhang Yan , Zhong Wenjun , Zhang Xiaowei , Pellissier Loïc , Altermatt Florian 
Published: November 2023

AbstractIn river systems worldwide, land cover changes have been identified as major drivers of biodiversity change. Quantifying how terrestrial land cover impacts riverine diversity requires local biodiversity assessments. In this study, we investigated the association of terrestrial land cover and the corresponding riverine fish species communities using eDNA‐metabarcoding in the Chinese Shaying River basin. This basin is home to about 37 million people and is largely dominated by a mix of intense agriculture and urban areas, creating a relatively homogeneous, intensely used landscape. We investigated whether the homogeneous landscape is mirrored in the composition and structure of fish communities in the river network. We sampled eDNA in spring and fall of 2018, amplified it with a primer designed for local fish species and used operational taxonomic units (OTU) assigned to fish as proxy for diversity. Furthermore, we used redundancy analysis, general linear models, and distance decay curves to assess the effects of land cover on fish communities. We found that the Shaying River showed relatively high basin‐wide richness (63 OTU) and seasonal differences in local richness, but limited community differentiation. Variations in alpha‐ and beta‐diversity, measured as local OTU richness and pairwise distance‐decay across the basin were low. Redundancy analysis showed only a weak association between observed aquatic communities and their terrestrial surroundings in a 10 km buffer upstream. The lack of community differentiation assessed by eDNA metabarcoding reflects the homogeneous and intense land‐use in this basin.
Survival of environmental DNA in sediments: Mineralogic control on DNA taphonomy
Authors: Freeman C. L. , Dieudonné L. , Agbaje O. B. A. , Žure M. , Sanz J. Q. , Collins M. , Sand K. K. 
Published: November 2023

AbstractThe extraction of environmental DNA (eDNA) from sediments is providing ground‐breaking views of past ecosystems and biodiversity. Despite this rich source of information, it is still unclear which sediments favor preservation and why. Here, we used atomic force microscopy and molecular dynamics simulations to explore the DNA‐mineral interaction to assess how mineralogy and interfacial geochemistry play a role in the preservation of environmental DNA on mineral substrates. We demonstrate that mineral composition, surface topography, and surface charge influence DNA adsorption behavior as well as preservation. Modeling and experimental data show that DNA damage can be induced by mineral binding if there is a strong driving force for adsorption. The study shows that knowledge of the mineralogical composition of a sediment and the environmental conditions can be useful for assessing if a deposit is capable of storing extracellular DNA and to what extent the DNA would be preserved. Our data adds to the understanding of eDNA taphonomy and highlights that, for some mineral systems, fragmented DNA may not represent old DNA.
Ecotoxicity and Risk to Zooplankton of a Novel Insecticide Used in Rice Paddies in a Simulated Ecosystem
Authors: Chen Lang , Yuan Shankui , Cai Leiming , Tang Baohua , Zhou Yanming , Zhou Xin Xin , Huang Chengtian , Zhao Yu , Yang Hairong , Lan Shuai 
Published: November 2023

Abstract
Diamide insecticides are widely used in rice paddies and pose a potential threat to aquatic organisms. However, the risk research related to their application in major rice-producing areas is very limited, especially mesocosm research to simulate the impact on aquatic ecosystems of long-term exposure, as well as exposure analysis based on local models and local scenarios. To assess potential risks from a novel diamide insecticide (tetrachlorantraniliprole) to aquatic nontarget organisms in the field over long-term exposure, an outdoor mesocosm study was performed, and the environmental concentrations were predicted by the multimedia paddy-pond model (TOPRICE). The mesocosm experiment showed that tetrachlorantraniliprole mainly stayed in the aqueous phase after entering the water body. Although the chemical dissipated quickly in the aqueous phase (half-life of 0.79–1.5 days), it showed toxic effects on zooplankton communities. Cladocerans, represented by Simocephalus vetulus, were most sensitive to tetrachlorantraniliprole stress. Significant short-term toxicity to cladocerans occurred in all treatment groups, but all recovered within 8 weeks except for the highest concentration group (30.0 µg /L). Based on the ecological recovery results, 7.74 µg tetrachlorantraniliprole/L (nominal concentration, 10.0 µg /L) is suggested to be the no-observed-ecological-adverse-effect concentration (NOEAEC) for the zooplankton community. When this NOEAEC was compared with predicted environmental concentrations (PECs; the PECs in natural ponds simulated by the TOPRICE model for 148 application scheme combinations in major rice-producing areas), a relatively high risk of applying tetrachlorantraniliprole during the rice tillering stage was found. The present study makes a positive contribution to the hypothesis that the current Tier 1 approaches for global acute risk assessment have a sufficient protective effect for assessing the risk of tetrachlorantraniliprole to aquatic organisms. Also, the present results should help us to gain a fuller understanding of the ecological risk of diamide insecticides in aquatic ecosystems and their rational application schemes. Environ Toxicol Chem 2024;43:429–439. © 2023 SETAC
Long‐term recruitment of peripheral immune cells to brain scars after a neonatal insult
Authors: Bolini Lukas , Campos Raquel Maria Pereira , Spiess Daiane Aparecida , Lima‐Rosa Frederico Luis , Dantas Danillo Pereira , Conde Luciana , Mendez‐Otero Rosalia , Vale Andre M , Pimentel‐Coelho Pedro Moreno 
Published: November 2023

AbstractAlthough brain scars in adults have been extensively studied, there is less data available regarding scar formation during the neonatal period, and the involvement of peripheral immune cells in this process remains unexplored in neonates. Using a murine model of neonatal hypoxic–ischemic encephalopathy (HIE) and confocal microscopy, we characterized the scarring process and examined the recruitment of peripheral immune cells to cortical and hippocampal scars for up to 1 year post‐insult. Regional differences in scar formation were observed, including the presence of reticular fibrotic networks in the cortex and perivascular fibrosis in the hippocampus. We identified chemokines with chronically elevated levels in both regions and demonstrated, through a parabiosis‐based strategy, the recruitment of lymphocytes, neutrophils, and monocyte‐derived macrophages to the scars several weeks after the neonatal insult. After 1 year, however, neutrophils and lymphocytes were absent from the scars. Our data indicate that peripheral immune cells are transient components of HIE‐induced brain scars, opening up new possibilities for late therapeutic interventions.
The influence of non‐financial enterprises' financialization on total factor productivity of enterprises: Promotion or inhibition?
Authors: Lyu Yanwei , Zhang Jinning , Qing Xinnian , Bai Yangyang 
Published: November 2023

AbstractIn recent years, the increasing trend of financialization of non‐financial enterprises and the improvement of total factor productivity (TFP) have attracted extensive attention. The research sample for this study covers non‐financial firms listed on the Shanghai and Shenzhen stock exchanges from 2008 to 2020. Then an econometric regression model is built to examine the impact of financialization of non‐financial enterprises on enterprises' TFP. The mediation effect model is used to investigate the mechanisms of non‐financial enterprises' financialization (NFEF) on TFP. The heterogeneity in investment efficiency, property rights and political affiliation is further discussed. The findings show that the NFEF has a considerable negative influence on enterprises' TFP, which still stands after endogenous treatment and a series of robustness tests. Moreover, NFEF significantly inhibits TFP by crowding out R&D investment and significantly promotes TFP by alleviating financing restrictions. Both the ‘crowding‐out effect’ and ‘reservoir effect’ of financialization of non‐financial enterprises are verified, and the former is greater. The influence of NFEF on TFP has the heterogeneity in investment efficiency and political affiliation, but the heterogeneity in property rights is insignificant.
Reducing the transcriptional read‐through rate of a lentiviral vector for β‐thalassemia gene therapy
Authors: Wu Jiahui , Chen Yuan , Shen Wenchen , Zhang Jingzhi , Zeng Fanyi 
Published: November 2023

AbstractBackgroundLentiGlobin BB305 is a self‐inactivating lentiviral vector carrying a human β‐globin expressing cassette for treating β‐thalassemia. Initially, a 2 × 250 bp chicken Locus Control Region fragment of cHS4, functioning as an insulator, was placed at its ΔU3, which was removed after the first clinical trial led by a French team to avoid abnormal splicing, etc. This action could potentially lead to an increasing risk of the transcriptional read‐through rate driven by the β‐globin promoter to a significant level, posing a biosafety risk in clinical trials.MethodsIn the present study, a read‐through reducing agent (C‐U+ or WPRE) was designed to be placed at the 3′ UTR of the β‐globin gene. The Enhancer Activities and/or Transcriptional Read‐Through (EATRT) rate at the mRNA level and the protein expression level regarding lentiviral preparation titer were examined.ResultsWe found that the insertion of the element (C‐U+ or WPRE) reduced the EATRT effectively by 53% or 41%, respectively. C‐U+ has less impact on virus package efficiency. Furthermore, there was no significant difference in the protein expression level after the C‐U+ or WPRE insertion.ConclusionsThe results of the present study show that inserting C‐U+ or WPRE before the polyA sequence of the BB305 would reduce the EATRT rate at no cost of its expressing efficacy and viral preparation titers. Thus, we present an alternative improvement for a safer lentiviral vector for β‐thalassemia clinical trials.
Revisiting semistrong edge‐coloring of graphs
Authors: Lužar Borut , Mockovčiaková Martina , Soták Roman 
Published: November 2023

AbstractA matching in a graph is semistrong if every edge of has an endvertex of degree one in the subgraph induced by the vertices of . A semistrong edge‐coloring of a graph is a proper edge‐coloring in which every color class induces a semistrong matching. In this paper, we continue investigation of properties of semistrong edge‐colorings initiated by Gyárfás and Hubenko. We establish tight upper bounds for general graphs and for graphs with maximum degree 3. We also present bounds about semistrong edge‐coloring which follow from results regarding other, at first sight nonrelated, problems. We conclude the paper with several open problems.