bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting post-translational modifications of Foxp3: a new paradigm for regulatory T cell-specific therapy
Authors: Riaz Farooq , Huang Zhihui , Pan Fan 
Published: October 2023

A healthy immune system is pivotal for the hosts to resist external pathogens and maintain homeostasis; however, the immunosuppressive tumor microenvironment (TME) damages the anti-tumor immunity and promotes tumor progression, invasion, and metastasis. Recently, many studies have found that Foxp3+ regulatory T (Treg) cells are the major immunosuppressive cells that facilitate the formation of TME by promoting the development of various tumor-associated cells and suppressing the activity of effector immune cells. Considering the role of Tregs in tumor progression, it is pivotal to identify new therapeutic drugs to target and deplete Tregs in tumors. Although several studies have developed strategies for targeted deletion of Treg to reduce the TME and support the accumulation of effector T cells in tumors, Treg-targeted therapy systematically affects the Treg population and may lead to the progression of autoimmune diseases. It has been understood that, nevertheless, in disease conditions, Foxp3 undergoes several definite post-translational modifications (PTMs), including acetylation, glycosylation, phosphorylation, ubiquitylation, and methylation. These PTMs not only elevate or mitigate the transcriptional activity of Foxp3 but also affect the stability and immunosuppressive function of Tregs. Various studies have shown that pharmacological targeting of enzymes involved in PTMs can significantly influence the PTMs of Foxp3; thus, it may influence the progression of cancers and/or autoimmune diseases. Overall, this review will help researchers to understand the advances in the immune-suppressive mechanisms of Tregs, the post-translational regulations of Foxp3, and the potential therapeutic targets and strategies to target the Tregs in TME to improve anti-tumor immunity.
Distribution and structure of deep-sea demersal fish assemblages across the central and western Pacific Ocean using data from undersea imagery
Authors: Selig Gina M. , Drazen Jeffrey C. , Auster Peter J. , Mundy Bruce C. , Kelley Christopher D. 
Published: October 2023

Demersal deep-sea fish assemblages from islands and seamounts are poorly described, even in the Hawaiian archipelago. Knowledge across all depths, in similar settings, is even sparser for other archipelagos in the central and western Pacific. However, recent remotely operated vehicle (ROV) explorations and archived video from human-occupied submersible dives conducted by the Hawai`i Undersea Research Laboratory (HURL) provide an opportunity to explore the structure of these assemblages. Here we describe demersal fish assemblages across the central and western Pacific, including in four Marine National Monuments, and examine the relationship of the assemblages to depth and environmental conditions. We used data collected from 227 underwater vehicle dives resulting in the identification of 24,837 individuals belonging to 89 families and 175 genera. The most frequently occurring genera at depths of 250-500 m were Epigonus, Setarches, Polymixia, and Antigonia, between 500-1000 m were Chlorophthalmus, Aldrovandia, and Neocyttus, and between 1000-3000 m were Synaphobranchus, Kumba, Halosaurus, Ilyophis, and Ipnops. There are strong changes in the fish assemblages with depth and region, and assemblages become more similar between regions with greater depth. Depth and region explained the most variance in assemblage structure followed by seafloor particulate organic carbon flux (a food supply proxy), concentrations of dissolved oxygen, and salinity. The Line Islands and Tokelau Ridge had the highest values of seafloor particulate organic carbon flux for all depth zones investigated (250-3000 m) and the highest abundance of fishes at 250-500 m and 500-1000 m, respectively. Taxon accumulation curves indicated that diversity at the genus level within all regions and depth bins (except 1000-2000 m and 2000-3000 m) had not been reached with the existing sampling effort. However, when combining samples from all regions, diversity generally appeared to decrease with depth. Overall, this study demonstrates that there are significant regional differences in the composition of the deep-sea fish fauna as well as differences across depth. Such distribution patterns suggest that the four Marine National Monuments (Papahānaumokuākea, Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments, encompassing an area of 3,063,223 km2) are not replicates of diversity, but complementary components of the regional fauna.
Accessible art in healthcare facilities: exploring perspectives of healthcare art for visually impaired people
Authors: Palityka Daryia , Chrysikou Evangelia , Murtagh Niamh 
Published: October 2023

IntroductionArt in healthcare facilities shows promising results in improving patients' health and well-being and, as such, meets the WHO's definition of health technology. Yet, it remains unclear if healthcare art equally benefits all users. Given the growing number of visually impaired people (VIP), it is valuable to determine whether healthcare art is accessible to VIP and to explore strategies for improving it.MethodsThis study employed a mixed methodology, which included (1) secondary research of 25 cases of healthcare art programmes to identify the presence of accessible art in healthcare facilities and the practices that influence it; (2) review of thirty-one Health Building Notes and four supplementary British guidelines on healthcare art to discover if the accessibility of art is required and identify which recommendations influence it; and (3) interview surveys of healthcare art practitioners from three London NHS Trusts to identify opportunities to increase arts accessibility.Results and discussionThe evidence showed that healthcare art programmes were mostly inaccessible to VIP. Most healthcare art programmes did not involve VIP in the commissioning process and, thus, lacked procedures that could facilitate accessibility. There were not enough recommendations in the healthcare facility guidelines to support the accessibility of arts for VIP. The recommendations on artwork in healthcare facility guidelines could increase accessibility if particular conditions were met. Interviews with NHS trusts in London revealed numerous opportunities to improve arts accessibility for healthcare art programmes.
Aromatic compounds depurative and plant growth promotion rhizobacteria abilities of Allenrolfea vaginata (Amaranthaceae) rhizosphere microbial communities from a solar saltern hypersaline soil
Authors: Rodríguez-Valdecantos Gustavo , Torres-Rojas Felipe , Muñoz-Echeverría Sofía , del Rocío Mora-Ruiz Merit , Rosselló-Móra Ramon , Cid-Cid Luis , Ledger Thomas , González Bernardo 
Published: October 2023

IntroductionThis work investigates whether rhizosphere microorganisms that colonize halophyte plants thriving in saline habitats can tolerate salinity and provide beneficial effects to their hosts, protecting them from environmental stresses, such as aromatic compound (AC) pollution.MethodsTo address this question, we conducted a series of experiments. First, we evaluated the effects of phenol, tyrosine, 4-hydroxybenzoic acid, and 2,4-dichlorophenoxyacetic (2,4-D) acids on the soil rhizosphere microbial community associated with the halophyteAllenrolfea vaginata. We then determined the ability of bacterial isolates from these microbial communities to utilize these ACs as carbon sources. Finally, we assessed their ability to promote plant growth under saline conditions.ResultsOur study revealed that each AC had a different impact on the structure and alpha and beta diversity of the halophyte bacterial (but not archaeal) communities. Notably, 2,4-D and phenol, to a lesser degree, had the most substantial decreasing effects. The removal of ACs by the rhizosphere community varied from 15% (2,4-D) to 100% (the other three ACs), depending on the concentration.Halomonasisolates were the most abundant and diverse strains capable of degrading the ACs, with strains ofMarinobacter,Alkalihalobacillus,Thalassobacillus,Oceanobacillus, and the archaeaHaladaptatusalso exhibiting catabolic properties. Moreover, our study found that halophile strains Halomonas sp. LV-8T andMarinobactersp. LV-48T enhanced the growth and protection ofArabidopsis thalianaplants by 30% to 55% under salt-stress conditions.DiscussionThese results suggest that moderate halophile microbial communities may protect halophytes from salinity and potential adverse effects of aromatic compounds through depurative processes.
Endovascular treatment for young patients with acute large vessel occlusion stroke in China: analysis of the ANGEL-ACT registry
Authors: Han Bin , Raynald  , Sun Dapeng , Tong Xu , Jia Baixue , Wang Anxin , Mo Dapeng , Gao Feng , Ma Ning , Nguyen Thanh N. , Miao Zhongrong 
Published: October 2023

BackgroundThe incidence of acute ischemic stroke caused by large vessel occlusion is relatively infrequent in the young adult population. We sought to evaluate their clinical outcomes after endovascular treatment (EVT) and stroke etiology compared with older patients.MethodsWe examined data from the ANGEL-ACT registry, a nationwide study in China focusing on EVT for acute ischemic stroke. We compared two age groups: &lt;50 years old and ≥50 years old. Our analysis focused on outcome measures such as the 90-day modified Rankin Scale (mRS) score, mortality, and symptomatic intracranial hemorrhage (sICH). We adjusted for confounding variables.ResultsWe included 1,691 patients, and 216 patients (13%) were &lt;50 years old. Young patients had lower median National Institutes of Health Stroke Scale (NIHSS) scores (14 vs. 17, P &lt; 0.001) and fewer cardiovascular comorbidities than older patients. Underlying intracranial atherosclerosis disease (ICAD) was higher in young patients (39.4 vs. 28.7%, P = 0.001). Clinical outcome was less favorable in older compared to younger patients (mRS shift: 0.76 [95% confidence interval (CI), 0.58–0.99]); functional independence [mRS score 0–2] 61% vs. 39% (adjusted odds ratio (OR), 0.7 [95% CI, 0.51–0.97]). Mortality and sICH did not differ between groups. Onset to puncture time (OTP) was longer in young patients (357 min vs. 294 min, P = 0.001).ConclusionAn estimated 13% of patients who underwent endovascular thrombectomy for acute ischemic stroke were &lt;50 years old. Symptomatic underlying ICAD was more prevalent in the younger patient population. Despite a prehospital delay, younger patients exhibited more favorable outcomes than their older counterparts.
Biopsy-proven acute tubulointerstitial nephritis in patients treated with immune checkpoint inhibitors: a pooled analysis of case reports
Authors: Esposito Pasquale , Bottini Annarita , Lecini Elvina , Cappadona Francesca , Piaggio Michela , Macciò Lucia , Genova Carlo , Viazzi Francesca 
Published: October 2023

IntroductionAcute kidney injury (AKI) in cancer patients receiving immune checkpoint inhibitors (ICIs) may recognize multiple causes. Here, we reviewed cases of biopsy-proven acute tubulointerstitial nephritis (ATIN) to describe the clinical characteristics and outcomes of this condition.MethodWe conducted a pooled analysis of clinical cases of ICI-related biopsy-proven ATIN up to 1 May 2022. We collected data on clinical characteristics, AKI, biopsy findings, laboratory examinations, and renal outcomes.ResultsEighty-five patients (61.4 ± 19 years, 56 male) were evaluated. Melanoma was the most prevalent diagnosis (51%), followed by non-small cell lung cancer (30%). ICI treatment consisted of PD-1, PDL-1 (nivolumab, pembrolizumab, atezolizumab), and CTLA-4 inhibitors (i) (ipilimumab) or combination PD-1i+CTLA4i. Renal toxicity developed after a median of four cycles of therapy. Fifty-one patients (65.5%) developed the most severe form of AKI- stage 3, including five patients requiring dialysis. All the 19 patients treated with dual ICI blockade developed AKI-stage 3, compared with 29 patients out of the 60 receiving a single agent (p&lt;0.001). Most events were managed with corticosteroids associated with ICI withdrawal. In 15 patients ICI was restarted, but in six (40%) AKI recurred. Overall, 32 patients (40%) presented a complete renal recovery, which chance was inversely associated with dual ICI blockade (OR 0.15, 95CI 0.03-0.7, p=0.01).ConclusionICI-related ATIN may develop late after the therapy initiation, presenting as severe AKI, particularly in patients with dual ICI blockade. Although this complication may be partially reversible, concerns remain about the renal function sequelae and the possibility of restarting ICI treatment.
IGF2BP2 promotes head and neck squamous carcinoma cell proliferation and growth via the miR-98-5p/PI3K/Akt signaling pathway
Authors: Yu Dan , Xiao Zhenlong , Zou Zhefei , Lin Ling , Li Jing , Tan Jian , Chen Wei 
Published: October 2023

IntroductionAs a N6-methyladenosine reader protein, Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a critical player in tumor progression and metastasis. However, its specific function in head and neck squamous carcinoma (HNSCC) has yet to be determined. The present study aimed to determine the role of IGF2BP2 in HNSCC.MethodsThe expression of IGF2BP2 in HNSCC was analyzed using The Cancer Genome Atlas (TCGA) dataset and detected in HNSCC tissues and cells, respectively. Gain- and loss- of function methods were employed to study the effects of IGF2BP2 on HNSCC cell proliferation and tumorigenesis in vitro and in vivo. MicroRNAs (miRNAs) regulating IGF2BP2 were predicted using online tools and confirmed experimentally.ResultsWe showed augmented IGF2BP2 expression in HNSCC, which correlated with poor clinical outcomes. Functional studies showed that IGF2BP2 promoted HNSCC cell proliferation by facilitating cell cycle progression while inhibiting apoptosis. We further demonstrated that IGF2BP2 could enhance HNSCC cell tumorigenesis in vivo. Mechanistically, our data revealed that miR-98-5p could directly target IGF2BP2. The interplay between IGF2BP2 and miR-98-5p is essential to drive the progression of HNSCC via the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-protein kinase B (Akt) pathway signaling pathway.DiscussionThe current study revealed the oncogenic role of IGF2BP2 and provided insights into its potential mechanism in HNSCC tumorigenesis. Additionally, IGF2BP2 might represent a promising therapeutic target and serve as prognostic biomarker in patients with HNSCC.
A comparative study on the features of breast sclerosing adenosis and invasive ductal carcinoma via ultrasound and establishment of a predictive nomogram
Authors: Li Yuan , Wei Xiu-liang , Pang Kun-kun , Ni Ping-juan , Wu Mei , Xiao Juan , Zhang Lu-lu , Zhang Fei-xue 
Published: October 2023

ObjectiveTo analyze the clinical and ultrasonic characteristics of breast sclerosing adenosis (SA) and invasive ductal carcinoma (IDC), and construct a predictive nomogram for SA.Materials and methodsA total of 865 patients were recruited at the Second Hospital of Shandong University from January 2016 to November 2022. All patients underwent routine breast ultrasound examinations before surgery, and the diagnosis was confirmed by histopathological examination following the operation. Ultrasonic features were recorded using the Breast Imaging Data and Reporting System (BI-RADS). Of the 865 patients, 203 (252 nodules) were diagnosed as SA and 662 (731 nodules) as IDC. They were randomly divided into a training set and a validation set at a ratio of 6:4. Lastly, the difference in clinical characteristics and ultrasonic features were comparatively analyzed.ResultThere was a statistically significant difference in multiple clinical and ultrasonic features between SA and IDC (P&lt;0.05). As age and lesion size increased, the probability of SA significantly decreased, with a cut-off value of 36 years old and 10 mm, respectively. In the logistic regression analysis of the training set, age, nodule size, menopausal status, clinical symptoms, palpability of lesions, margins, internal echo, color Doppler flow imaging (CDFI) grading, and resistance index (RI) were statistically significant (P&lt;0.05). These indicators were included in the static and dynamic nomogram model, which showed high predictive performance, calibration and clinical value in both the training and validation sets.ConclusionSA should be suspected in asymptomatic young women, especially those younger than 36 years of age, who present with small-size lesions (especially less than 10 mm) with distinct margins, homogeneous internal echo, and lack of blood supply. The nomogram model can provide a more convenient tool for clinicians.
Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles
Authors: Yates Devyn , Di Maggio Lucia S. , Rosa Bruce A. , Sprung Robert W. , Erdmann-Gilmore Petra , Townsend R. Reid , Budge Philip J. , Kamgno Joseph , Mitreva Makedonka , Weil Gary J. , Fischer Peter U. 
Published: October 2023

BackgroundImproved diagnostic tools are needed for detecting active filarial infections in humans. Tests are available that detect adult W. bancrofti circulating filarial antigen, but there are no sensitive and specific biomarker tests for brugian filariasis or loiasis. Here we explored whether extracellular vesicles released by filarial parasites contain diagnostic biomarker candidates.MethodsVesicles were isolated using VN96-affinity purification from supernatants of short-term in vitro cultured B. malayi microfilariae (Mf) and analyzed by mass spectrometry (Bruker timsTOF). Parasite-specific proteins were identified by bioinformatic analysis and a protein was called present if supported by ≥ 2 spectra. After validation with cultures parasites, this approach was then used to analyze vesicles isolated from plasma of animals infected with B. malayi and from humans with heavy Loa loa infections.ResultsVesicles from Mf cultures contained more than 300 B. malayi proteins with high consistency across biological replicates. These included the known Mf excretory antigen BmR1 (AF225296). Over 150 B. malayi proteins were detected in vesicles isolated from plasma samples from two infected animals. Vesicles isolated from plasma from 10 persons with high L. loa Mf densities contained consistently 21 proteins, 9 of them were supported by at least 5 unique peptides and 7 with spectral counts above 10. The protein EN70_10600 (an orthologue of the B. malayi antigen BmR1, GenBank AF225296) was detected in all 10 samples with a total count of 91 spectra and a paralogue (EN70_10598) was detected in 6 samples with a total of 44 spectra.DiscussionExtracellular vesicles released by filarial parasites in vitro and in vivo contain parasite proteins which can be reliably detected by mass spectrometry. This research provides the foundation to develop antigen detection assays to improve diagnosis of active filarial infections in humans.
A classification method of stress in plants using unsupervised learning algorithm and chlorophyll fluorescence technology
Authors: Lu Miao , Gao Pan , Hu Jin , Hou Junying , Wang Dong 
Published: October 2023

IntroductionChilling injury is one of the most common meteorological disasters affecting cucumber production. For implementing remedial measures as soon as possible to minimize production loss, a timely and precise assessment of chilling injury is crucial.MethodsTo evaluate the possibility of detecting cucumber chilling injury using chlorophyll fluorescence (ChlF) technology, we investigated the continuous changes in ChlF parameters under various low-temperature conditions and created the criteria for evaluating chilling injury. The ChlF induction curves were first collected before low-temperature as unstressed samples and daily 1 to 5 days after low-temperature as chilling injury samples. Principal component analysis was employed to investigate the public information on ChlF parameters and evaluate the differences between samples with different degrees of chilling injury. The parameters (Fv/Fm, Y(NO), qP, and Fo) accounted for a large proportion in the principal components and could characterize chilling injury. Uniform manifold approximation and projection method was employed to extract new features (Feature 1, Feature 2, Feature 3, and Feature 4) from ChlF parameters for subsequent classification model. Taking four features as input, a classification model based on the Fuzzy C-means clustering algorithm was constructed in order to identify the chilling injury classes of cucumber seedlings. The cucumber seedlings with different chilling injury classes were analyzed for ChlF images, rapid light curves, and malondialdehyde content.Results and discussionThe results demonstrated that the variations in these indicators among the different chilling injury classes supported the validity of the classification model. Our findings provide a better understanding of the relationship between ChlF parameters and the impact of low-temperature treatment on cucumber seedlings. This finding offers an additional perspective that can be used to evaluate the responses and damage that plants experience under stress.
Exploring the relationships among safety leadership, safety climate, psychological contract of safety, risk perception, safety compliance, and safety outcomes
Authors: Omidi Leila , Karimi Hossein , Pilbeam Colin , Mousavi Saeid , Moradi Gholamreza 
Published: October 2023

BackgroundRecently, two types of safety compliance behaviors including deep compliance and surface compliance were differentiated. The current study aimed to investigate the relationships among safety leadership, safety climate, psychological contract of safety (PCS), risk perception, and deep compliance and surface compliance behavior of workers. In addition, the effects of both deep and surface compliance on safety outcomes were considered.MethodsWorkers’ perceptions in terms of safety leadership, safety climate, PCS, risk perception, deep compliance, and surface compliance were measured by appropriate questionnaires. Three questions were asked to measure undesired safety outcomes. Structural equation modeling and correlation analysis were applied to examine the research model and relationships among variables.Results and discussionThe results of the current study showed that deep compliance was positively predicted by safety leadership, safety climate, and PCS and negatively predicted by risk perception. Surface compliance was positively predicted by safety leadership and safety climate and negatively predicted by risk perception. Surface compliance is not significantly predicted by PCS. With regard to the adverse safety outcomes, the results showed that both deep and surface compliance were negatively associated with adverse safety outcomes, however, deep compliance had a stronger negative correlation with adverse safety outcomes than surface compliance.