bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardioprotective Action of a Novel Synthetic 19,20-EDP Analog Is Sirt Dependent
Authors: Kranrod Joshua W. , Darwesh Ahmed M. , Bassiouni Wesam , Huang Andy , Fang Liye , Korodimas Jacob V. , Adebesin Adeniyi Michael , Munnuri Sailu , Falck John R. , Seubert John M. 
Published: November 2023

Abstract:
Mounting evidence suggests that cytochrome P450 epoxygenase–derived metabolites of docosahexaenoic acid, called epoxydocosapentaenoic acids (EDPs), limit mitochondrial damage after cardiac injury. In particular, the 19,20-EDP regioisomer has demonstrated potent cardioprotective action. Thus, we investigated our novel synthetic 19,20-EDP analog SA-22 for protection against cardiac ischemia–reperfusion (IR) injury. Isolated C57BL/6J mouse hearts were perfused through Langendorff apparatus for 20 minutes to obtain baseline function, followed by 30 minutes of global ischemia. Hearts were then treated with vehicle, 19,20-EDP, SA-22, or SA-22 with the pan-sirtuin inhibitor nicotinamide or the SIRT3-selective inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP) at the start of 40 minutes reperfusion (N = 5–8). We assessed IR injury–induced changes in recovery of myocardial function, using left ventricular developed pressure and systolic and diastolic pressure change. Tissues were assessed for electron transport chain function, SIRT1 and SIRT3, optic atrophy type 1, and caspase-1. We also used H9c2 cells in an in vitro model of hypoxia/reoxygenation injury (N = 3–6). Hearts perfused with SA-22 had significantly improved postischemic left ventricular developed pressure, systolic and diastolic recovery (64% of baseline), compared with vehicle control (15% of baseline). In addition, treatment with SA-22 led to better catalytic function observed in electron transport chain and SIRT enzymes. The protective action of SA-22 resulted in reduced activation of pyroptosis in both hearts and cells after injury. Interestingly, although nicotinamide cotreatment worsened functional outcomes, cell survival, and attenuated sirtuin activity, it failed to completely attenuate SA-22–induced protection against pyroptosis, possibly indicating EDPs exert cytoprotection through pleiotropic mechanisms. In short, these data demonstrate the potential of our novel synthetic 19,20-EDP analog, SA-22, against IR/hypoxia-reoxygenation injury and justify further development of therapeutic agents based on 19,20-EDP.
Affinity purification-mass spectrometry and single fiber physiology/proteomics reveals mechanistic insights of C18ORF25
Authors: Ng Yaan-Kit , Blazev Ronnie , McNamara James W. , Dutt Mriga , Molendijk Jeffrey , Porrello Enzo R. , Elliott David A. , Parker Benjamin L. 
Published: November 2023

AbstractC18ORF25 was recently shown to be phosphorylated at S67 by AMP-activated protein kinase (AMPK) in skeletal muscle following acute exercise in humans. Phosphorylation was shown to improveex vivoskeletal muscle contractile function in mice but our understanding of the molecular mechanisms is incomplete. Here, we profiled the interactome of C18ORF25 in mouse myotubes using affinity purification coupled to mass spectrometry. This analysis included an investigation of AMPK-dependent and S67-dependent protein:protein interactions. Several nucleocytoplasmic and contractile-associated proteins were identified, and revealed a subset of GTPases that associate with C18ORF25 in an AMPK- and S67 phosphorylation-dependent manner. We confirmed that C18ORF25 is localised to the nucleus and the contractile apparatus in skeletal muscle. Mice lackingC18Orf25display defects in calcium handling specifically in fast-twitch muscle fibers. To investigate these mechanisms, we developed an integrated single fiber physiology and single fiber proteomic platform. The approach enabled detailed assessment of various steps in the excitation-contraction (EC) pathway including SR calcium handling and force generation followed by paired single fiber proteomic analysis. This enabled us to identify >700 protein:phenotype associations and 36 fiber-type specific differences following loss ofC18Orf25. Taken together, our data provide unique insights into the function of C18ORF25 and its role in skeletal muscle physiology.
Moving together – Benefits of a 12-week online dance training intervention on static and dynamic postural stability and gait speed in older adults – a pre-post study
Authors: Hansen Rasmus Kopp , Jochum Elizabeth , Egholm Ditte , Villumsen Morten , Hirata Rogerio Pessoto 
Published: November 2023

AbstractBackgroundPhysical inactivity negatively affects gait performance and postural stability in older adults, which results in a higher risk of fall accidents. Previous research has shown that in-person dance training improves various aspects of balance and lower extremity function, however, the potential benefits of dance training delivered online on variables used for fall risk stratification in older adults have yet to be studied. We aimed to explore the benefits of a 12-week online dance training intervention on static and dynamic postural stability and gait speed in older adults.MethodsForty-five older adults were included. The 12-week dance training intervention consisted of two weekly 60-min classes in improvisation and salsa delivered online through Zoom video calls. Static and dynamic postural stability was assessed using the center of pressure (CoP) area and velocity (force platform), and the Mini Balance Evaluation Systems Test (Mini-BESTest), respectively. 10-m gait speed was measured using photo gates. Before and after comparisons were performed using paired sample t-tests.ResultsThirty-two older adults completed the study. There were no significant changes for static postural stability assessed by CoP area or velocity (P ≥ 0.218). The Mini-BESTest total score was significantly improved at post-intervention (23.88 ± 3.01) compared to baseline (22.56 ± 1.41, P = 0.007). 10-m gait speed was significantly faster at post-intervention (1.68 ± 0.25 m/s) compared to baseline (1.57 ± 0.22 m/s, P < 0.001).DiscussionThese results indicate that online dance training has limited effect on static postural stability but may be beneficial for gait speed and in particular dynamic postural control among older adults. While the absolute increase in gait speed observed in the present study suggests limited clinical relevance, the improvement in Mini-BESTest score was above the minimal clinically important difference, which suggests a clinically relevant enhancement of dynamic postural control.
The Risk factors for fear of falling in chronic stroke patients: a systematic review and meta-analysis
Authors: Kong Yun , Zhong Kelong , An Xuemei 
Published: November 2023

AbstractBackgroundThe incidence rate of fear of falling in chronic stroke is high, which seriously affects the quality of life and rehabilitation effect of patients. Early identification of its risk factors will help clinical screening of high-risk patients and prevent their further development. There is currently no systematic evaluation of risk factors for fear of falling falls in chronic stroke.ObjectiveWe systematically reviewed the literature on risk factors for fear of fall in chronic patients with stroke.MethodWe systematically searched PubMed, Embase,cochrane,Web of Science and the China National Knowledge Infrastructure(CNKI),the china biomedical literature database(CBM),the China Science and Technology Periodicals Database(VIP),Wangfang data for relevant literature until May 2023.Review Manager V.5.3 merged the OR value and 95% CI of the potential risk factors.A random/fixed-effect meta-analysis was used to pool risk factors from individual studies.Cochran’s Q and the I2tests were used to assess heterogeneity between the studies.ResultsA total of 6 studies were included for the final analysis, with 965 chronic stroke patients. The risk factors for fear of falling in chronic patients with stroke were impaired balance ability (OR=3.05; 95% CI 1.60 to 5.80), history of falls (OR=2.12; 95% CI 1.40 to 3.20) and anxiety (OR=2.29; 95% CI 1.43 to 3.67), depression (OR=1.80; 95% CI 1.22 to 2.67), poor lower limb motor function (OR=1.14; 95% CI 1.00 to 1.29),physically inactiveness (OR=2.04; 95% CI 1.01 to 4.12). Married (OR=0.61; 95% CI: 0.435-0.875) is a protective factor.ConclusionOur study shows that impaired balance ability, history of falls might be a greater risk for fear of falling. Future studies are recommended to determine other risk factors specific to patients with chronic stroke.
Association between gut microbiome composition and symptom self-report in trauma exposed OEF/OIF/OND Veterans
Authors: Li Y. Irina , Pagulayan Kathleen , Rau Holly , Hendrickson Rebecca , Schindler Abigail G. 
Published: November 2023

AbstractObjectiveIraq and Afghanistan war-era (OEF/OIF/OND) Veterans are at elevated risk for physical injuries and psychiatric illnesses, in particular comorbid mild traumatic brain injury (mTBI), posttraumatic stress disorder (PTSD), and chronic pain. The gut microbiome has been implicated in modulation of critical processes such as digestion, immune system functioning, and stress responsivity, and may be an important factor in understanding physical and mental health outcomes following deployment and trauma exposure, yet minimal research to date has sought to characterize gut microbiome composition in this population.Methods26 male OEF/OIF/OND Veterans aged 18 to 65 who previously completed a VA Comprehensive TBI Evaluation were enrolled in this study. Participants completed self-report measures of PTSD symptom severity, pain intensity and interference, fatigue, cognitive symptoms, substance use, and sleep quality. Participants submitted fecal samples, and metagenomic sequencing was used to calculate alpha- and beta-diversity and taxonomic microbial composition. Associations between microbiome data and clinical variables was then examined.ResultsAlpha and beta diversity measures were not significantly correlated with clinical outcomes. Fatigue, post-concussive symptoms, executive function symptoms, and cannabis use were associated with differences in gut microbial composition, specifically Verrucomicrobiota.ConclusionThis exploratory study demonstrated that altered gut microbiome composition is associated with psychiatric and cognitive symptoms in OEF/OIF/OND Veterans and highlights a potential new therapeutic target of interest. Future research is needed to examine whether probiotic treatment is effective for reducing symptoms common in this clinical population.
Business unit controllers' credibility and the hardening of local forecasts
Authors: Wiegmann Leona , Petrikowski Lukas , Goretzki Lukas 
Published: November 2023

AbstractFocusing on multidivisional companies, this paper analyzes the hardening of local forecasts at the intersection of business units (BUs) and the corporate finance function. It investigates how BU controllers, accountable to both local management and the corporate finance function, seek to establish themselves as competent and trustworthy forecasters vis‐à‐vis their functional superiors. Drawing on Goffman's dramaturgical sociology, we demonstrate how these encounters constitute episodes in a multiperiod hardening game feeding into the management of forecast quality and anticipatory control. We illustrate how expressive performances of their competence and trustworthiness are vital for BU controllers to manage vertical information flows between the local and the corporate level and for aligning corresponding interests. Convincing performances can reinforce BU controllers' status as stewards of the forecasting process and help to maintain a “truce” between the local and the corporate level, assuring corporate controllers that the unit's future is under control.
Cytologic evaluation of upper urinary tract specimens: An institutional retrospective study using The Paris System for Reporting Urine Cytology second edition with histopathologic follow‐up
Authors: Khajir Ghazal , Sun Tong , Wang He , Sprenkle Preston C. , Adeniran Adebowale J. , Cai Guoping , Levi Angelique W. 
Published: November 2023

AbstractObjectiveCytologic evaluation of the upper urinary tract (UUT) can be challenging due to instrumentation artefacts. This study retrospectively reviewed UUT specimens using The Paris System for Reporting Urinary Cytopathology, second edition (TPS 2.0), compared it with the original reporting system (ORS) and correlated it with histopathologic follow‐up.MethodsAn institutional database was reviewed for the UUT biopsy/resection histopathologic specimens, and we included 52 UUT cytology specimens pertinent to these cases in the study. These specimens were blindly reviewed and reclassified using TPS 2.0. The correlation between TPS 2.0, ORS and histopathologic follow‐up was assessed.ResultsThe UUT cytology specimens corresponded to 21 (40.4%) high‐grade urothelial carcinoma (HGUC), 27 (51.9%) low‐grade urothelial carcinoma (LGUC) and 4 (7.7%) benign cases on follow‐up. For HGGC cases, the associated TPS categories included unsatisfactory (n = 1, 4.8%), negative for HGUC (NHGUC; n = 3, 14.3%), atypical urothelial cells (AUC; n = 6, 28.6%), suspicious for HGUC (SHGUC; n = 3, 14.3%) and HGUC (n = 8, 38.1%), while ORS categorised the specimens as unsatisfactory (n = 1, 4.8%), negative for malignant cells (NFMC; n = 3, 14.3%), AUC (n = 5, 23.8%), low‐grade urothelial carcinoma (LGUC; n = 0, 0%), SHGUC (n = 5, 23.8%) and HGUC (n = 7, 33.3%). The risks of high‐grade malignancy among cytologic categories were similar between ORS and TPS (p > 0.05). The majority of LGUC were classified as AUC similarly by ORS and TPS (55.6% vs. 59.3%).ConclusionsOur study demonstrated comparable performance between TPS 2.0 and ORS for UUT cytology specimens. Cytological diagnosis of UUT specimens remains challenging, especially for LGUC.
Multi‐criteria evolutionary optimization of a traffic light using genetics algorithm and teaching‐learning based optimization
Authors: Yektamoghadam Hossein , Nikoofard Amirhossein , Behzadi Masoumeh , Khosravy Mahdi , Dey Nilanjan , Witkowski Olaf 
Published: November 2023

AbstractToday, the development of urbanization and increasing the number of vehicles has resulted in displeased consequences like traffic congestion and vehicle queuing. The vast majority of countries in the world encounter the challenge of the explosive rise in traffic demand. In this regard, it is necessary to meet traffic demand in transport networks, especially in metropolitans. In traffic management and shortening the trip duration, traffic lights on the signalized intersections play an essential role in urban pathways. This work provides a multi‐criteria decision‐making method for optimum traffic light control in an isolated corner. The main idea involves establishing a set of sub‐optimal solutions for traffic light timing and selecting the best one among the diverse solutions. We have mathematically modelled the problem as an optimization problem to achieve an optimal solution with less waiting time for vehicles in intersections and the lowest cost. Genetic algorithm (GA) and Teaching‐Learning‐based Optimization (TLBO) are utilized for each phase to create a set of suitable timing scenarios. The Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method is used to identify the best scenario, considering both waiting vehicles and traffic capacity as decision criteria. Its efficiency has been demonstrated over three different traffic volumes. Also, in a real‐world implementation, its practical capability has been approved at a crossroads in Mashhad, Iran. The simulations indicate the improvement in the number of vehicles waiting behind the crossroad and the traffic capacity by 10% and 6.76% compared to the existing signal timing of the studied intersection, respectively.
Learning Chinese as a foreign language: An introduction
Authors: House Juliane , Kádár Dániel Z. 
Published: November 2023

AbstractIn this introductory paper, we first present the background of the present special issue dedicated to Willis Edmondson. We first point out why Edmondson provided a ground‐breaking contribution to the field of applied linguistics and why it is particularly timely to edit a special issue centering on his framework. We also argue that Edmondson's bottom‐up and strictly language‐anchored view on speech acts and interaction is particularly useful to examine the learning of Chinese as a foreign language, by going beyond exoticizing and overgeneralizing views of the Chinese linguaculture. Second, we briefly present what can be regarded as the heart and soul of the Edmondsonian framework, that is, a typology of speech acts and a related procedure through which the relationship between speech acts in interaction can be captured. Third, we present a research procedure that we outlined in our previous work, and which helps implementing the Edmondsonian model in the pragmatic study of foreign language learning. Finally, we present the contents of the special issue.
Juvenile hormone inhibits adult cuticle formation in Drosophila melanogaster through Kr‐h1/Dnmt2‐mediated DNA methylation of Acp65A promoter
Authors: He Qianyu , Fan Xiaochun , Wang Shunxin , Chen Shanshan , Chen Jinxia 
Published: November 2023

AbstractDifferentiation of imaginal epidermal cells of Drosophila melanogaster to form adult cuticles occurs at approximately 40–93 h after puparium formation. Juvenile hormone (JH) given at pupariation results in formation of a second pupal cuticle in the abdomen instead of the adult cuticle. Although the adult cuticle gene Acp65A has been reported to be down‐regulated following JH treatment, the regulatory mechanism remains unclear. Here, we found that the JH primary response gene Krüppel homologue 1 (Kr‐h1) plays a vital role in the repression of adult cuticle formation through the mediation of JH action. Overexpression of Kr‐h1 mimicked—while knocking down of Kr‐h1 attenuated—the inhibitory action of JH on the formation of the adult abdominal cuticle. Further, we found that Kr‐h1 inhibited the transcription of Acp65A by directly binding to the consensus Kr‐h1 binding site (KBS) within the Acp65A promoter region. Moreover, the DNA methyltransferase Dnmt2 was shown to interact with Kr‐h1, combined with the KBS to promote the DNA methylation of sequences around the KBS, in turn inhibiting the transcription of Acp65A. This study advances our understanding of the molecular basis of the “status quo” action of JH on the Drosophila adult metamorphosis.
Genetic and context-specific effects on individual inhibitory control performance in the guppy (Poecilia reticulata)
Authors: Prentice Pamela M. , Thornton Alex , Kolm Niclas , Wilson Alastair J. 
Published: November 2023

Abstract
Among-individual variation in cognitive traits, widely assumed to have evolved under adaptive processes, is increasingly being demonstrated across animal taxa. As variation among individuals is required for natural selection, characterizing individual differences and their heritability is important to understand how cognitive traits evolve. Here, we use a quantitative genetic study of wild-type guppies repeatedly exposed to a ‘detour task’ to test for genetic variance in the cognitive trait of inhibitory control. We also test for genotype-by-environment interactions (GxE) by testing related fish under alternative experimental treatments (transparent vs. semi-transparent barrier in the detour-task). We find among-individual variation in detour task performance, consistent with differences in inhibitory control. However, analysis of GxE reveals that heritable factors only contribute to performance variation in one treatment. This suggests that the adaptive evolutionary potential of inhibitory control (and/or other latent variables contributing to task performance) may be highly sensitive to environmental conditions. The presence of GxE also implies that the plastic response of detour task performance to treatment environment is genetically variable. Our results are consistent with a scenario where variation in individual inhibitory control stems from complex interactions between heritable and plastic components.
Abstract
We use a quantitative genetic study to examine variation in cognitive performance in wild-type guppies. We show among-individual variation in detour task performance, consistent with differences in inhibitory control as well as plasticity to visual information context. Heritable variation was also detected but GxE means this is limited to the low information treatment. Our results suggest the adaptive evolutionary potential of inhibitory control may be highly sensitive to environmental context.
Diagnostic work‐up and surgical management of insulinoma: A retrospective analysis from a tertiary referral center
Authors: Andreasi Valentina , Partelli Stefano , Muffatti Francesca , Battistella Anna , Fermi Francesca , Balzano Gianpaolo , Crippa Stefano , Tamburrino Domenico , Pecorelli Nicolò , De Cobelli Francesco , Arcidiacono Paolo Giorgio , Falconi Massimo 
Published: November 2023

AbstractInsulinoma is a multifaceted disease that poses several challenges in terms of clinical presentation, diagnostic work‐up, and surgical management. The aim of this study was to describe diagnostic work‐up, surgical management, and postoperative outcomes of patients with insulinoma. All consecutive patients who underwent surgery for insulinoma at San Raffaele Hospital (Milan, Italy) between January 2008 and January 2022 were included. Overall, 98 patients were considered. The median delay between presenting symptoms and insulinoma diagnosis was 10 months (IQR, 4–21). Insulinoma diagnosis was made at our Institution in 45 patients, 20 of whom referred within 6 months from symptoms onset. In this subgroup, the median interval between symptoms presentation and insulinoma diagnosis was 4 months (IQR, 2–6), as compared to 14 months (IQR, 10–26) in patients (n = 25) who referred to our institution after 6 months from symptoms onset (p < .001). The insulinoma was localized preoperatively in all the cases. All patients underwent ≥1 high‐quality imaging: computed tomography (CT: n = 87, sensitivity 84%), magnetic resonance imaging (MRI: n = 55, sensitivity 85%) and endoscopic ultrasound (EUS: n = 79, sensitivity 100%). MRI identified the tumor in eight patients with negative CT. EUS localized the insulinoma in three patients with negative CT and negative MRI. Parenchyma‐sparing resections were performed in 41 patients. Contact with major vessels, lesion close to Wirsung duct and suspect of malignancy were the main reasons to perform a formal resection. An early referral to high‐volume centers is important for reducing diagnostic delay in patients with insulinoma. The diagnostic work‐up of insulinoma frequently requires several imaging modalities to be performed, with EUS being the most sensitive one. Parenchyma‐sparing surgery for insulinoma should be performed whenever technically and oncologically feasible.
Fracturing walls, not identities: The power of John Green's fully human representations of disability in The Fault in Our Stars
Authors: Pearson Amy 
Published: November 2023

AbstractIn my paper, I argue that young‐adult author John Green recognizes the problematic ways conventional literary representations of people with disabilities contribute to the dehumanization of people with impairments. Furthermore, I suggest that Green's vibrant characters in The Fault in Our Stars and the connection readers have formed with them illuminate the power that realistic and, therefore, necessarily complex representations of characters with disabilities hold in transforming how people approach others with impairments, and, ultimately, how people with disabilities construct their own identities.