bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immersive virtual reality and computer vision for heritage: visual evaluation and perception of the industrial heritage sites along the Yunnan–Vietnam railway (Yunnan section)
Authors: Wang Yingxue , Wang Shuoyi , Pan Yue , Li Chunhua , Chen Chunxu , Wang Jin 
Published: February 2024

Abstract
The visual composition and human perception are found to relate to the reuse and tourism of heritage railways. Previous studies have used either environmental audits and on-site interviews that have limitations in terms of cost, time, and measurement scale, or virtual perception base on two-dimension images but with gaps in interactivity, virtual immersion and field of view. This study developed an “objective + subjective” visual evaluation and perception framework integrating Computer Vision (CV) and Immersive Virtual Reality (IVR) to assess the visual quality of industrial heritage sites along the Yunnan-Vietnam Railway (Yunnan Section). The stepwise multiple linear regression models were carried out to investigate the relationship between objective evaluation and subjective perception. The results showed that 16 landscape elements of the heritage sites were successfully segmented. According to the visual perception score bands, the 120 industrial heritage sites were classified as 39 high-score sites, 66 medium-score sites, and 15 low-score sites. In general, although the sky and hard ground accounted for a higher proportion, they had little effect on the sum scores, while the vegetation, water, and buildings played a significant role in the perception of visual quality. The results can help researchers, planners, and government departments clarify the visual quality to scientifically specify bottom-up planning and management solutions for railway industrial heritage sites. Moreover, the simplicity, accuracy, and effectiveness of this framework make it suitable for large-scale visual evaluation of other railway industrial heritage sites and linear heritage sites.
The beneficial endophytic microbes enhanced tobacco defense system to resist bacterial wilt disease
Authors: Tao Jiemeng , Gu Mengli , Yu Shizhou , Shi Jingjing , Cheng Lingtong , Jin Jingjing , Lu Peng , Zhang Jianfeng , Li He , Cao Peijian 
Published: February 2024

Abstract
Background
The vital role of rhizosphere microbiome in protecting plants against pathogen infection has been well characterized. By contrast, beneficial effects of the plant endophytic microbiome and interactions with plants remain poorly understood. Here, we integrated microbial community analysis, culture-based methods and plant defense gene quantification to systematically investigate the responses of endophytic root and stem microbiomes and the defense system of the tobacco plant to Ralstonia solanacearum, a bacterial pathogen causing bacterial wilt disease worldwide.

Results
An obvious reduction of community diversity and changes of microbial composition were observed in tobacco root and stem compartments upon R. solanacearum infection. Beneficial endophytic microbes were enriched in both diseased root and stem compartments, and beneficial microbes enriched in roots were more diverse than those in stems. Several beneficial bacteria enriched in diseased roots showed positive correlations with the pathogen abundance and exhibited great antagonistic activities against R. solanacearum. At the same time, the expression of key genes participating in plant defense signaling pathways and activities of plant defense enzymes were activated with R. solanacearum infection. Inoculation of beneficial endophyte Burkholderia ASV_550 in soils could greatly enhance the tobacco defense system in the presence of pathogen and decrease the disease incidence.

Conclusions
The recruited beneficial microbes exhibited an enhancement of the defense system of host plants. This work provides endophyte evidence for the “cry for help” strategy in plants and advances the current understanding of plant–microbe interactions in resisting plant disease.

Graphical Abstract
Effects of metal corrosion in the pump of a dialysis machine on the sterility of the terminal dialysate by spike-and-recovery testing with bacteria
Authors: Nakamura Minoru , Okayama Masaya , Hagiwara Seya , Nawa Tomoyasu , Yokota Shin-ichi 
Published: February 2024

Abstract
Background
Dialysis units have been concerned that the corroded metal parts of pumps used in hemodialysis might not allow sterility of the pump to be ensured due to bacterial contamination, even after cleaning and disinfection are performed after dialysis treatment. The purpose of this study was to clarify the effectiveness of the cleaning/disinfection process in eliminating bacterial contamination of the dialysate in pumps with and without metal corrosion.

Methods
A suspension of Pseudomonas aeruginosa [10 colony-forming unit (CFU)/mL] was introduced into pumps without or with corrosion of the metal parts, and the flow in the dialysis circuit was stopped for 6, 12, or 18 h. Then, after cleaning and disinfection of the circuit with a sodium-hypochlorite-containing reagent, the amounts of live bacteria in the terminal dialysate and on the surface of the metal parts of the pump were counted.

Results
Irrespective of the presence or absence of metal corrosion, bacteria were detected, even after cleaning and disinfection, on the surfaces of the pump parts that had been left in contact with the bacterial suspension for more than 12 h. However, on the surfaces of the pump parts showing metal corrosion, the bacterial numbers increased dramatically after 18 h of flow stoppage time following introduction of bacteria, and bacteria were even detected in the terminal dialysate despite cleaning/disinfection of the pump.

Conclusions
Corrosion of the metal parts used in pumps used for dialysis increases the risk of bacterial contamination of not only the pump parts and flow path of the dialysis machine but also the terminal dialysate, even if cleaning/disinfection is performed. For sterility assurance of the dialysis circuit and dialysate during routine use, it is necessary to eliminate corrosion of the metal parts of dialysis pumps during scheduled maintenance.
Monomodular and multifunctional processive endocellulases: implications for swine nutrition and gut microbiome
Authors: Fan Ming Z. , Cheng Laurence , Wang Min , Chen Jiali , Fan Wenyi , Jashari Fatmira , Wang Weijun 
Published: February 2024

AbstractPoor efficiency of dietary fibre utilization not only limits global pork production profit margin but also adversely affects utilization of various dietary nutrients. Poor efficiency of dietary nutrient utilization further leads to excessive excretion of swine manure nutrients and results in environmental impacts of emission of major greenhouse gases (GHG), odor, nitrate leaching and surface-water eutrophication. Emission of the major GHG from intensive pork production contributes to global warming and deteriorates heat stress to pigs in tropical and sub-tropical swine production. Exogenous fibre enzymes of various microbial cellulases, hemicellulases and pectinases have been well studied and used in swine production as the non-nutritive gut modifier feed enzyme additives in the past over two decades. These research efforts have aimed to improve growth performance, nutrient utilization, intestinal fermentation as well as gut physiology, microbiome and health via complementing the porcine gut symbiotic microbial fibrolytic activities towards dietary fibre degradation. The widely reported exogenous fibre enzymes include the singular use of respective cellulases, hemicellulases and pectinases as well as their multienzyme cocktails. The currently applied exogenous fibre enzymes are largely limited by their inconsistent in vivo efficacy likely due to their less defined enzyme stability and limited biochemical property. More recently characterized monomodular, multifunctional and processive endoglucanases have the potential to be more efficaciously used as the next-generation designer fibre biocatalysts. These newly emerging multifunctional and processive endoglucanases have the potential to unleash dietary fibre sugar constituents as metabolic fuels and prebiotics, to optimize gut microbiome, to maintain gut permeability and to enhance performance in pigs under a challenged environment as well as to parallelly unlock biomass to manufacture biofuels and biomaterials.
Medicaid Insurance is a Predictor of Prolonged Hospital Length of Stay After Traumatic Brain Injury: A Stratified National Trauma Data Bank Cohort Analysis of 552 949 Patients
Authors: Yue John K. , Ramesh Rithvik , Krishnan Nishanth , Chyall Lawrence , Halabi Cathra , Huang Michael C. , Manley Geoffrey T. , Tarapore Phiroz E. , DiGiorgio Anthony M. 
Published: February 2024

BACKGROUND AND OBJECTIVES:
Hospital length of stay (HLOS) is a metric of injury severity, resource utilization, and healthcare access. Recent evidence has shown an association between Medicaid insurance and increased HLOS after traumatic brain injury (TBI). This study aims to validate the association between Medicaid and prolonged HLOS after TBI using the National Trauma Data Bank.

METHODS:
National Trauma Data Bank Trauma Quality Programs Participant Use Files (2003-2021) were queried for adult patients with TBI using traumatic intracranial injury ICD-9/ICD-10 codes. Patients with complete HLOS, age, sex, race, insurance payor, Glasgow Coma Scale, Injury Severity Score, and discharge disposition data were included (N = 552 949). Analyses were stratified by TBI severity using Glasgow Coma Scale. HLOS was coded into Tiers according to percentiles within TBI severity categories (Tier 1: 1-74th; 2: 75-84th; 3: 85-94th; 4: 95-99th). Multivariable logistic regressions evaluated associations between insurance payor and prolonged (Tier 4) HLOS, controlling for sociodemographic, Injury Severity Score, cranial surgery, and discharge disposition variables. Adjusted odds ratios (aOR) and 95% CI were reported.

RESULTS:
HLOS Tiers consisted of 0–19, 20–27, 28–46, and ≥47 days (Tiers 1-4, respectively) in severe TBI (N = 103 081); 0–15, 16–21, 22–37, and ≥38 days in moderate TBI (N = 39 904); and 0–7, 8–10, 11–19, and ≥20 days in mild TBI (N = 409 964). Proportion of Medicaid patients increased with Tier ([Tier 1 vs Tier 4] severe: 16.0% vs 36.1%; moderate: 14.1% vs 31.6%; mild TBI: 10.2% vs 17.4%; all P < .001). On multivariable analyses, Medicaid was associated with prolonged HLOS (severe TBI: aOR = 2.35 [2.19-2.52]; moderate TBI: aOR = 2.30 [2.04-2.61]; mild TBI: aOR = 1.75 [1.67-1.83]; reference category: private/commercial).

CONCLUSION:
This study supports Medicaid as an independent predictor of prolonged HLOS across TBI severity strata. Reasons may include different efficacies in care delivery and reimbursement, which require further investigation. Our findings support the development of discharge coordination pathways and policies for Medicaid patients with TBI.
Improved Side-Effect Stimulation Thresholds and Postoperative Transient Confusion With Asleep, Image-Guided Deep Brain Stimulation
Authors: Kesarwani Rohit , Mahajan Uma V. , Wang Alexander S. , Kilbane Camilla , Shaikh Aasef G. , Miller Jonathan P. , Sweet Jennifer A. 
Published: February 2024

BACKGROUND AND OBJECTIVES:
Asleep, image-guided deep brain stimulation (DBS) is a modern alternative to awake, microelectrode recording (MER) guidance. Studies demonstrate comparable efficacy and complications between techniques, although some report lower stimulation thresholds for side effects with image guidance. In addition, few studies directly compare the risk of postoperative transient confusion (pTC) across techniques. The purpose of this study was to compare clinical efficacy, stimulation thresholds for side effects, and rates of pTC with MER-guided DBS vs intraoperative 3D-fluoroscopy (i3D-F) guidance in Parkinson's disease and essential tremor.

METHODS:
Consecutive patients from 2006 to 2021 were identified from the departmental database and grouped as having either MER-guided DBS or i3D-F–guided DBS insertion. Directional leads were used once commercially available. Changes in Unified Parkinson's Disease Rating Scale (UPDRS)-III scores, levodopa equivalent daily dose, Fahn–Tolosa–Marin scores, and stimulation thresholds were assessed, as were rates of complications including pTC.

RESULTS:
MER guidance was used to implant 487 electrodes (18 globus pallidus interna, GPi; 171 subthalamic nucleus; 76 ventrointermediate thalamus, VIM) in 265 patients. i3D-F guidance was used in 167 electrodes (19 GPi; 25 subthalamic nucleus; 41 VIM) in 85 patients. There were no significant differences in Unified Parkinson's Disease Rating III Scale, levodopa equivalent daily dose, or Fahn–Tolosa–Marin between groups. Stimulation thresholds for side effects were higher with i3D-F guidance in the subthalamic nucleus (MER, 2.80 mA ± 0.98; i3D-F, 3.46 mA ± 0.92; P = .002) and VIM (MER, 2.81 mA ± 1.00; i3D-F, 3.19 mA ± 1.03; P = .0018). Less pTC with i3D-F guidance (MER, 7.5%; i3D-F, 1.2%; P = .034) was also found.

CONCLUSION:
Although clinical efficacy between MER-guided and i3D-F–guided DBS was comparable, thresholds for stimulation side effects were higher with i3D-F guidance and the rate of pTC was lower. This suggests that image-guided DBS may affect long-term side effects and pose a decreased risk of pTC.
A Drosophila model of mitochondrial disease phenotypic heterogeneity
Authors: Granat Lucy , Knorr Debbra Y. , Ranson Daniel C. , Chakrabarty Ram Prosad , Chandel Navdeep S. , Bateman Joseph M. 
Published: February 2024

ABSTRACT
Mutations in genes that affect mitochondrial function cause primary mitochondrial diseases. Mitochondrial diseases are highly heterogeneous and even patients with the same mitochondrial disease can exhibit broad phenotypic heterogeneity, which is poorly understood. Mutations in subunits of mitochondrial respiratory complex I cause complex I deficiency, which can result in severe neurological symptoms and death in infancy. However, some complex I deficiency patients present with much milder symptoms. The most common nuclear gene mutated in complex I deficiency is the highly conserved core subunit NDUFS1. To model the phenotypic heterogeneity in complex I deficiency, we used RNAi lines targeting the Drosophila NDUFS1 homolog ND-75 with different efficiencies. Strong knockdown of ND-75 in Drosophila neurons resulted in severe behavioural phenotypes, reduced lifespan, altered mitochondrial morphology, reduced endoplasmic reticulum (ER)-mitochondria contacts and activation of the unfolded protein response (UPR). By contrast, weak ND-75 knockdown caused much milder behavioural phenotypes and changes in mitochondrial morphology. Moreover, weak ND-75 did not alter ER-mitochondria contacts or activate the UPR. Weak and strong ND-75 knockdown resulted in overlapping but distinct transcriptional responses in the brain, with weak knockdown specifically affecting proteosome activity and immune response genes. Metabolism was also differentially affected by weak and strong ND-75 knockdown including gamma-aminobutyric acid (GABA) levels, which may contribute to neuronal dysfunction in ND-75 knockdown flies. Several metabolic processes were only affected by strong ND-75 knockdown including the pentose phosphate pathway and the metabolite 2-hydroxyglutarate (2-HG), suggesting 2-HG as a candidate biomarker of severe neurological mitochondrial disease. Thus, our Drosophila model provides the means to dissect the mechanisms underlying phenotypic heterogeneity in mitochondrial disease.
Assessing the benefits and management of urban forest in supporting low carbon city in Jakarta, Indonesia
Authors: AULIA RISDAYATRI , KASWANTO KASWANTO , ARIFIN HADI SUSILO , MOSYAFTIANI AMARIZNI , SYASITA NADA , WAHYU ADE , WIYOGA HARITYAS 
Published: February 2024

Abstract. Aulia R, Kaswanto, Arifin HS, Mosyaftiani A, Syasita N, Wahyu A, Wiyoga H. 2023. Assessing the benefits and management of urban forest in supporting low carbon city in Jakarta, Indonesia. Biodiversitas 24: 6151-6159. Climate change is a phenomenon that has become a global concern including in urban areas. Jakarta is one of the most populated cities in Indonesia, reaching 10.56 million people in 2020 with high emissions levels due to high human activities. Thus, efforts to reduce carbon emissions to mitigate climate change are necessary in Jakarta one of which is through the concept of low carbon city and manifested in the establishment of Green Open Space (GOS), including urban forests. Urban forests aim to sequester and store carbon, and improve the urban microclimate. This research aimed to assess the benefits of urban forests in Jakarta in term of their capacity in carbon sequestration using i-Tree Eco tool, and analyse the management of urban forests related to the applicable regulations. To collect data on trees, this study created 49 plots scattered across Srengseng Urban Forest, Cipayung Urban Forest, Munjul Urban Forest, Rawa Malang Urban Forest, and Pondok Labu Urban Forest. Tree species, Diameter at Breast Height (DBH), total tree height, live tree height, crown base height, crown width, percent crown loss, and crown light exposure were all gathered. Furthermore, in-depth interviews with urban forest managers were performed to gather additional information on present management, including questions derived from Jakarta's green open space legislation to assess management efficacy. The results of the analysis of urban forest quality show that the health status of trees is one of the factors that indicate the quality of urban forests at the five study sites, with more than 50% of the total trees in fair condition (75- 90% health). The gross carbon sequestration of trees in five urban forests Jakarta is about 184.8 metric tons of carbon per year. Additionally, the in-depth interviews also test the mortality of the urban forest, a decent indicator of current management with estimates over the next 10 years, showing a slight increase in carbon sequestration and storage capacity. However, the mortality scenario leads to a trend toward a decrease in leaf area and the number of tree categories, especially in the Srengseng Urban Forest.
Hostile healthcare? Initial insights into the landscape of charging policies in the NHS in Wales
Authors: Haigh Christine E. , Mogford Holly 
Published: February 2024

The NHS is now firmly positioned as a site of immigration control. As the Hostile Environment filters further into the NHS the principle of universality is increasingly disputed. As such, paradoxically, harm is reproduced through an institution which is intended to provide care. Despite the increasing breadth of recognition of the implications of charging migrants within the NHS in England, insights into specific practices within Wales are limited. Therefore, this research starts to address this paucity by providing initial insights into the extent of NHS charging within Wales.
The results from multiple freedom of information (FOI) requests sent to all seven health boards in Wales (carried out between January 2019 and August 2023) suggest that NHS treatment charging is common at scale across all health boards providing secondary care in Wales. In some instances, patients are being charged 150% of the cost of their treatment, and a significant number of patients are being incorrectly charged for care. It also appears that many patients have difficulty paying these charges, with significant outstanding invoices and many health boards resorting to using debt-collection agencies and/or payment plans in an attempt to elicit payment, and patients’ details being shared with the UK Home Office as a result.
Considering the harms which are produced through NHS charging regulations, campaigners and advocates including Patients Not Passports Wales call for charging regulations to be withdrawn from the NHS in Wales and across the UK.
Determinants of diabetic nephropathy among diabetic patients in Ethiopia: Systematic review and meta-analysis
Authors: Azagew Abere Woretaw , Beko Zerko Wako , Mekonnen Chilot Kassa 
Published: February 2024

Introduction
Diabetic nephropathy (DN) is a long-term kidney disease among diabetic patients. It is the leading cause of end-stage renal failure. In Ethiopia, DN affects the majority of diabetic populations, but there were inconsistent findings about the determinant factors across the studies.

Methods
We have accessed studies using PubMed, Embase, EBSCO, Web of Science, OVID, and search engines including Google and Google Scholar published up to June 2023. The study populations were diabetic patients with nephropathy. The quality of each included article was assessed using the Newcastle-Ottawa quality assessment scale. The odds ratios of risk factors were pooled using a random-effect meta-analysis model. Heterogeneity was assessed using the Cochrane Q statistics and I-Square (I2). The publication bias was detected using the funnel plot and/or Egger’s test (p< 0.05). Trim and fill analysis was carried out to treat the publication bias. The protocol has been registered with the reference number CRD42023434547.

Results
A total of sixteen articles were used for this reviewed study. Of which, eleven articles were used for advanced age, ten articles for duration of diabetic illness, ten articles for poor glycemic control, and eleven articles for having co-morbid hypertension. Diabetic patients with advanced age (AOR = 1.11, 95% CI: 1.03–120, I2 = 0.0%, p = 0.488), longer duration of diabetic illness (AOR = 1.23, 95% CI = 1.05–1.45, I2 = 0.0%, p = 0.567), poor glycemic control (AOR = 2.57, 95% CI: 1.07–6.14; I2 = 0.0%, p = 0.996), and having co-morbid hypertension (AOR = 4.03, 95% CI: 2.00–8.12, I2 = 0.0%, p = 0.964) were found to be factors associated with DN.

Conclusions
The findings of the study revealed that diabetic patients with advanced age, longer duration of diabetic illness, poor glycemic control status, and co-morbid hypertension were the determinant factors of DN. Therefore, treatment of co-morbid hypertension and high blood glucose and regular screening of renal function should be implemented to detect, treat, and reduce the progression of DN. Furthermore, healthcare workers should give due attention to diabetes with advanced age and a longer duration of diabetes illness to prevent the occurrence of DN.