bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosome-Level Genome Assembly and Annotation of a Periodical Cicada Species: Magicicada septendecula
Authors: Bush Jonas , Webster Cynthia , Wegrzyn Jill , Simon Chris , Wilcox Edward , Khan Ruqayya , Weisz David , Dudchenko Olga , Aiden Erez Lieberman , Frandsen Paul 
Published: January 2024

Abstract
We present a high-quality assembly and annotation of the periodical cicada species, Magicicada septendecula (Hemiptera: Auchenorrhyncha: Cicadidae). Periodical cicadas have a significant ecological impact, serving as a food source for many mammals, reptiles, and birds. Magicicada are well known for their massive emergences of 1 to 3 species that appear in different locations in the eastern United States nearly every year. These year classes (“broods”) emerge dependably every 13 or 17 yr in a given location. Recently, it has become clear that 4-yr early or late emergences of a sizeable portion of a population are an important part of the history of brood formation; however, the biological mechanisms by which they track the passage of time remain a mystery. Using PacBio HiFi reads in conjunction with Hi-C proximity ligation data, we have assembled and annotated the first whole genome for a periodical cicada, an important resource for future phylogenetic and comparative genomic analysis. This also represents the first quality genome assembly and annotation for the Hemipteran superfamily Cicadoidea. With a scaffold N50 of 518.9 Mb and a complete BUSCO score of 96.7%, we are confident that this assembly will serve as a vital resource toward uncovering the genomic basis of periodical cicadas’ long, synchronized life cycles and will provide a robust framework for further investigations into these insects.
Major Abdominal Surgery for Pediatric Crohn’s Disease in the Anti-TNF Era: 10-Year Analysis of Data From the IBD Registry of Italian Society of Pediatric Gastroenterology, Hepatology, and Nutrition
Authors: Alvisi Patrizia , Faraci Simona , Scarallo Luca , Congiu Marco , Bramuzzo Matteo , Illiceto Maria Teresa , Arrigo Serena , Romano Claudio , Zuin Giovanna , Miele Erasmo , Gatti Simona , Aloi Marina , Renzo Sara , Caldaro Tamara , Labriola Flavio , De Angelis Paola , Lionetti Paolo 
Published: January 2024

Abstract

Background
The natural history of Crohn’s disease (CD) can result in complications requiring surgery. Pediatric data are scarce about major abdominal surgery. The IBD Registry from the Italian Society of Pediatric Gastroenterology, Hepatology, and Nutrition has been active since 2008 and collects data from major pediatric IBD centers in Italy. The aim of the present report was to explore the prevalence of major abdominal surgery among children affected by CD in an era when antitumor necrosis factor (anti-TNF-α) agents were already used so that we might appraise the incidence of surgical-related complications and identify the factors associated with postoperative disease recurrence.

Methods
We retrospectively analyzed data from patients enrolled in the registry from January 2009 to December 2018. Patients with monogenic IBD and patients undergoing surgery for perianal disease were excluded.

Results
In total, 135 of 1245 patients were identified. We report the prevalence of major abdominal surgery of 10.8%. Pediatric surgeons performed the procedure in 54.1% of cases, and a laparoscopic approach was used in 47.4% of surgical procedures. Seventeen patients (12.6%) experienced a total of 21 early postoperative complications, none of which was severe. A laparoscopic approach was the only factor negatively associated with the occurrence of postoperative complications (odds ratio, 0.22; 95% confidence interval, 0.06-0.8; P = .02). Fifty-four (40%) patients experienced postoperative endoscopic recurrence, and 33 (24.4%) of them experienced postoperative clinical recurrence. The postoperative treatment with anti-TNF-α drugs was significantly associated with a reduced risk of endoscopic recurrence (odds ratio, 0.19; 95% confidence interval, 0.05-0.79; P = .02).

Conclusion
In our cohort, the overall prevalence of major abdominal surgery was low, as well as the rate of surgical-related complications. Postoperative anti-TNF-α therapy seems be protective against endoscopic recurrence.
Supplementing vitamins and minerals to beef heifers during gestation: impacts on mineral status in the dam and offspring, and growth and physiological responses of female offspring from birth to puberty
Authors: Hurlbert Jennifer L , Baumgaertner Friederike , Menezes Ana Clara B , Bochantin Kerri A , Diniz Wellison J S , Underdahl Sarah R , Dorsam Sheri T , Kirsch James D , Sedivec Kevin K , Dahlen Carl R 
Published: January 2024

Abstract
We evaluated the effects of feeding a vitamin and mineral supplement to nulliparous beef heifers throughout gestation on the mineral status of the dam, calf, placenta, and colostrum; offspring growth performance; and physiological responses of offspring raised as replacement heifers. Angus-based heifers (n = 31, initial body weight [BW] = 412.5 ± 53.68 kg) were adapted to an individual feeding system for 14 d, estrus synchronized and bred with female-sexed semen. Heifers were ranked by BW and randomly assigned to receive either a basal diet (CON; n = 14) or the basal diet plus 113 g heifer−1 d−1 of the vitamin and mineral supplement (VTM; n = 17). Targeted BW gains for both treatments was 0.45 kg heifer−1 d−1. Liver biopsies were obtained from dams at breeding, days 84 and 180 of gestation. At calving, liver biopsies were taken from dams and calves; colostrum, placenta, and blood samples were collected; and calf body measurements were recorded. After calving, all cow-calf pairs received a common diet through weaning, and F1 heifer calves were managed similarly after weaning. Offspring growth performance, feeding behavior, blood metabolites, and hormones were evaluated from birth through 15 mo of age. Data were analyzed using the MIXED procedure in SAS with repeated measures where appropriate. Hepatic concentrations of Se decreased in VTM dams (P ≤ 0.05) from day 84 to calving, while concentrations of Cu decreased in VTM and CON (P ≤ 0.05) from day 84 to calving. Calf liver concentrations of Se, Cu, Zn, and Co at birth were greater for VTM than CON (P ≤ 0.05), but calf birth BW and body measurements were not different (P = 0.45). Placental Se, colostrum quantity, total Se, Cu, Zn, and Mn in colostrum were greater (P ≤ 0.04) in VTM dams than CON. Finally, offspring from VTM dams were heavier than CON (P &lt; 0.0001) from weaning through 15 mo of age. These results were coupled with greater (P ≤ 0.04) blood glucose at birth, decreased (P ≤ 0.05) blood urea nitrogen at pasture turn out and weaning, and altered feeding behaviors in VTM offspring compared with CON. Maternal gestational vitamin and mineral supplementation enhanced mineral status in dams and F1 progeny, augmented postnatal offspring growth and blood metabolites. Consequently, in utero vitamin and mineral supplementation may exert programming outcomes on the performance and productivity of females raised as herd replacements and should be considered when developing diets for gestating cows and heifers.
Mer tyrosine kinase regulates bone metabolism, and its deficiency partially ameliorates periodontitis- and ovariectomy-induced bone loss in mice
Authors: Ryu Ka-Young , Pokhrel Nitin Kumar , Jung Hye-Jin , Kim Hyo Jeong , Seok Jiwon , Kim Tae-Young , Kim Hyung Joon , Lee Ji Hye , Kim Jae-Young , Kim Yong-Gun , Lee Youngkyun 
Published: January 2024

Abstract
Bone homeostasis is maintained by tightly coordinated activities of bone-forming osteoblasts and bone-resorbing osteoclasts. In the present report, the role of Mer tyrosine kinase (MerTK) in bone metabolism was investigated. The expression of MerTK decreased upon BMP2 stimulation of osteoblast precursors. The femurs of Mertk-deficient mice showed significantly increased bone volume with concomitant increase of bone formation and reduction in bone resorption. These bone phenotypes were attributed to the increased osteoblast differentiation and mineralization accounted by the enhanced β-catenin and Smad signaling in the absence of MerTK in osteoblast precursors. Although the Mertk-deficient bone marrow macrophages were predisposed to enhanced osteoclast differentiation via augmented Ca2+-NFATc1 signaling, the dramatic increase of Tnfsf11b/Tnfsf11 (Opg/Rankl) ratio in Mertk knockout bones and osteoblast precursors corroborated the reduction of osteoclastogenesis in Mertk deficiency. In ligature-induced periodontitis and ovariectomy models, the bone resorption was significantly attenuated in Mertk-deficient mice compared with wild-type control. Taken together, these data indicate novel role of MerTK in bone metabolism and suggest a potential strategy targeting MerTK in treating bone-lytic diseases including periodontitis and osteoporosis.
Peripheral canalicular branching is decreased in streptozotocin-induced diabetes and correlates with decreased whole-bone ultimate load and perilacunar elastic work
Authors: Bolger Morgan W , Tekkey Tara , Kohn David H 
Published: January 2024

Abstract
Osteocytes, the most abundant cell type in bone, play a crucial role in mechanosensation and signaling for bone formation and resorption. These cells reside within a complex lacuno-canalicular network (OLCN). Osteocyte signaling is reduced under diabetic conditions, and both type 1 and type 2 diabetes lead to reduced bone turnover, perturbed bone composition, and increased fracture risk. We hypothesized that this reduced bone turnover, and altered bone composition with diabetes is associated with reduced OLCN architecture and connectivity. This study aimed to elucidate: (1) the sequence of OLCN changes with diabetes related to bone turnover and (2) whether changes to the OLCN are associated with tissue composition and mechanical properties. Twelve- to fourteen-week-old male C57BL/6 mice were administered streptozotocin at 50 mg/kg for 5 consecutive days to induce hyperglycemia, sacrificed at baseline (BL), or after being diabetic for 3 (D3) and 7 (D7) wk with age-matched (C3, C7) controls (n = 10–12 per group). Mineralized femoral sections were infiltrated with rhodamine, imaged with confocal microscopy, then the OLCN morphology and topology were characterized and correlated against bone histomorphometry, as well as local and whole-bone mechanics and composition. D7 mice exhibited a lower number of peripheral branches relative to C7. The total number of canalicular intersections (nodes) was lower in D3 and D7 relative to BL (P &lt; 0.05 for all), and a reduced bone formation rate (BFR) was observed at D7 vs C7. The number of nodes explained only 15% of BFR, but 45% of Ct.BV/TV, and 31% of ultimate load. The number of branches explained 30% and 22% of the elastic work at the perilacunar and intracortical region, respectively. Collectively, the reduction in OLCN architecture and association of OLCN measures with bone turnover, mechanics, and composition highlights the relevance of the osteocyte and the OLCN and a potential therapeutic target for treating diabetic skeletal fragility.
Enzymatic activity and development of Frankliniella occidentalis (Thysanoptera: Thripidae) in response to exogenous calcium treatments of kidney bean plants
Authors: Qiu Xin-Yue , Huang Wan-Qing , Zeng Guang , Yue Wen-Bo , Zhang Chang-Yu , Zhi Jun-Rui 
Published: January 2024

Abstract
Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is an agricultural pest threatening various horticultural crops worldwide. Inducing plant resistance is an ecologically beneficial and potentially effective method for controlling F. occidentalis. As an essential nutrient element, exogenous calcium enhances plant-induced resistance. This study investigated the effects of CaCl2 on the secondary metabolites of kidney bean plants and detoxifying and digestive enzymes in F. occidentalis. We found that treatment of plants and treatment time and also the interactions of the 2 factors significantly affected secondary metabolites contents (tannin, flavonoids, total phenol, alkaloid, and lignin) of kidney bean leaves, which indicated that that the effect of treatment of plants on secondary metabolites varied with treatment time. Moreover, when thrips fed on CaCl2-treated plants, the activities of detoxifying enzymes, enzymes glutathione S-transferase and cytochrome P450 substantially increased compared to those in which thrips fed on control plants. However, the activity of carboxylesterase significantly decreased. The detoxifying enzyme genes CL992.contig6, CYP4PN1, and CYP4PJ2 were significantly upregulated at 24 and 48 h. The activities of digestive enzymes (α-amylase, chymotrypsin, and lipase) increased substantially in F. occidentalis. The digestive enzyme gene, FoAMY-1, was significantly upregulated at 24 and 48 h after treatment. The pupation rate and pupal weight of F. occidentalis were significantly reduced. The results indicated that exogenous CaCl2-induced metabolic changes in kidney bean plants and altered the enzymatic activity and development of F. occidentalis that fed upon them.
Characterizing the effect of longwall retreating speeds on overburden and surface synergistic subsidence behaviors with a thick coal seam: intensive field measurements
Authors: Wang Qiang , Ren Zhaopeng , Bai Qingsheng , Li Quansheng 
Published: January 2024

AbstractThe 12 401 longwall face in the Shangwan coal mine currently boasts one of the largest mining height (&gt;8.0 m) longwall faces in the world. In-depth field measurements, including overburden movement, surface subsidence and fissure, and shield support loads, were conducted to elucidate the subsidence and damage behaviors induced by high-intensity mining. The findings identified three control rock strata (CRS1, CRS2, and CRS3), which dominate the formation of two subsidence modes (‘two zones’ and ‘three zones’) depending on longwall retreating speed. CRS1 and CRS2 collectively affect the variation of shield support loads. CRS2 governs the movement of the entire overburden, and its rupture precipitates swift subsidence. CRS3, characterized by thick soft rock with robust expansion and bending resistances, serves as a buffer, mitigating displacements caused by the breakage of CRS2. It is instrumental in shaping the ‘three-zone’ overburden structure. At lower retreating speeds, the height of the water-conduction fracture zone (Hwf) is ∼200 m, contrasting with 120.47–134.46 m for higher retreating speeds. The CRS2 manifests as a ‘step rock beam’ at lower retreating speeds, causing a maximum surface subsidence of 6315 mm. Conversely, under higher retreating speeds, it adopts a ‘masonry beam’ structure, resulting in a maximum surface subsidence of 4947 mm. The distribution of ground fissures is significantly affected by retreating speed, aligning closely with the breakage steps of the control rock strata. Our study offers robust scientific insights into preventing mining damage and promoting ecologically sustainable mining practices.
Injection moulding process parameter and strain rate dependence mechanical properties measurement and theoretical estimation of EVA polymer foam
Authors: Tsang Yuk-Wah , Fu Hsiao-Ju , Huang Pei-Chen , Yadav Bishnu-Nand , Liu De-Shin 
Published: January 2024

Abstract
In this study, the combined experimental and theoretical estimation on elastic characteristics of EVA (ethylene-vinyl acetate) copolymer material is demonstrated. Strain rate dependence stress–strain behavior of EVA in raw and foam material form are measured by the quasi static and dynamic compression test. Simultaneously, the influence of different mould temperature and injection pressure on mechanical properties of EVA foam are investigated. According to the usage scenario of EVA foam in sport footwear application, the EVA foam is generally deformed under higher strain rate as compared with the quasi-static compression condition. For this reason, the stress–strain behavior of EVA foam under high strain rates of 2.66 × 10−3 and 2.66 × 10−2 s−1 are estimated. The analytic results revealed that the strain rate slightly enhanced the modulus of EVA foam, and the foregoing strain rate effect is substituting into the classic Young's modulus estimation equation of closed cell cellular solid. Accordingly, the strain rate dependence Young's modulus of EVA foam is successfully estimated by both experiment and theoretical estimation.
Enhanced induction of abnormal telomere FISH signals in response to oxidative DNA damage
Authors: Sakamoto Yoshimi , Shiraishi Kazunori , Kodama Seiji 
Published: January 2024

Abstract
Telomere dysfunction induces chromosomal instability, which is a driving force in the development of cancers. To examine X-irradiation’s effect on telomere integrity, we investigated X-ray-induced abnormalities in telomere signals detected by fluorescence in situ hybridization (telomere FISH) in mouse embryo fibroblast cells. The abnormalities were categorized as either extra telomere signals (ETSs) or loss of telomere signals (LTSs). The results indicated that low doses (0.3–0.5 Gy) of X-rays significantly induced ETS but not LTS and that ETS induction was saturated at doses above 0.5 Gy. In addition, treatment with hydrogen peroxide also induced ETS but not LTS. To clarify the involvement of radicals in inducing ETS, we examined the effect of ascorbic acid (AsA) on telomere FISH signals and found that pre-treatment with AsA (5 mM, 2 h), but not post-treatment, significantly suppressed the induction of ETS by X-irradiation. Importantly, neither pre- nor post-treatment with AsA affected X-ray-induced chromosome aberrations. These results suggest that oxidative DNA damage induced by radicals is involved in the induction of ETS. Furthermore, combined treatment with aphidicolin, a DNA replication inhibitor, elevated the induction of ETS by X-irradiation. This observation suggests that DNA replication stress, potentially triggered by oxidative DNA lesions within telomeres, may contribute to the induction of ETS resulting from X-irradiation. Based on these results, we propose that ETS is a sensitive biological marker of oxidative DNA damage in telomere structures.
Transcription factors RhbZIP17 and RhWRKY30 enhance resistance to Botrytis cinerea by increasing lignin content in rose petals
Authors: Li Dandan , Li Xiaomei , Wang Zicheng , Wang Haochen , Gao Junzhao , Liu Xintong , Zhang Zhao 
Published: January 2024

Abstract
The petals of ornamental plants such as roses (Rosa spp.) are the most economically important organs. This delicate, short-lived plant tissue is highly susceptible to pathogens, in large part because the walls of petal cells are typically thinner and more flexible compared with leaf cells, allowing the petals to fold and bend without breaking. The cell wall is a dynamic structure that rapidly alters its composition in response to pathogen infection, thereby reinforcing its stability and boosting plant resistance against diseases. However, little is known about how dynamic changes in the cell wall contribute to resistance to Botrytis cinerea in rose petals. Here, we show that the B. cinerea-induced transcription factor RhbZIP17 is required for the defense response of rose petals. RhbZIP17 is associated with phenylpropanoid biosynthesis and binds to the promoter of the lignin biosynthesis gene RhCAD1, activating its expression. Lignin content showed a significant increase under gray mold infection compared with the control. RhCAD1 functions in the metabolic regulation of lignin production and, consequently, disease resistance, as revealed by transient silencing and overexpression in rose petals. The WRKY transcription factor RhWRKY30 is also required to activate RhCAD1 expression and enhance resistance against B. cinerea. We propose that RhbZIP17 and RhWRKY30 increase lignin biosynthesis, improve the resistance of rose petals to B. cinerea, and regulate RhCAD1 expression.
A joint SRG/eROSITA + ZTF search: Discovery of a 97-min period eclipsing cataclysmic variable with evidence of a brown dwarf secondary
Authors: Galiullin Ilkham , Rodriguez Antonio C , Kulkarni Shrinivas R , Sunyaev Rashid , Gilfanov Marat , Bikmaev Ilfan , Yungelson Lev , van Roestel Jan , Gänsicke Boris T , Khamitov Irek , Szkody Paula , El-Badry Kareem , Suslikov Mikhail , Prince Thomas A , Buntov Mikhail , Caiazzo Ilaria , Gorbachev Mark , Graham Matthew J , Gumerov Rustam , Irtuganov Eldar , Laher Russ R , Medvedev Pavel , Riddle Reed , Rusholme Ben , Sakhibullin Nail , Sklyanov Alexander , Vanderbosch Zachary P 
Published: January 2024

ABSTRACT
Cataclysmic variables (CVs) that have evolved past the period minimum during their lifetimes are predicted to be systems with a brown dwarf donor. While population synthesis models predict that around 40–70 per cent of the Galactic CVs are post-period minimum systems referred to as ‘period bouncers’, only a few dozen confirmed systems are known. We report the study and characterization of a new eclipsing CV, SRGeJ041130.3+685350 (SRGeJ0411), discovered from a joint SRG/eROSITA and ZTF programme. The optical spectrum of SRGeJ0411 shows prominent hydrogen and helium emission lines, typical for CVs. We obtained optical high-speed photometry to confirm the eclipse of SRGeJ0411 and determine the orbital period to be Porb ≈ 97.530 min. The spectral energy distribution suggests that the donor has an effective temperature of ≲ 1800 K. We constrain the donor mass with the period–density relationship for Roche lobe-filling stars and find that Mdonor ≲ 0.04 M⊙. The binary parameters are consistent with evolutionary models for post-period minimum CVs, suggesting that SRGeJ0411 is a new period bouncer. The optical emission lines of SRGeJ0411 are single-peaked despite the system being eclipsing, which is typically only seen due to stream-fed accretion in polars. X-ray spectroscopy hints that the white dwarf in SRGeJ0411 could be magnetic, but verifying the magnetic nature of SRGeJ0411 requires further investigation. The lack of optical outbursts has made SRGeJ0411 elusive in previous surveys, and joint X-ray and optical surveys highlight the potential for discovering similar systems in the near future.
The rotation curve and mass distribution of M31
Authors: Zhang Xiangwei , Chen Bingqiu , Chen Pinjian , Sun Jiarui , Tian Zhijia 
Published: January 2024

ABSTRACT
To gain a better understanding of the Andromeda galaxy M31 and its role in the Local Group, measuring its mass precisely is essential. In this work, we have constructed the rotation curve of M31 out to ∼125 kpc using 13 679 M31 objects obtained from various sources, including the LAMOST data release 9 (LAMOST DR9), the DESI survey, and relevant literature. We divide all objects in our sample into bulge, disc, and halo components. For the sources in the M31 disc, we have measured their circular velocities by a kinematic model with asymmetric drift corrections. For the bulge and halo objects, we calculate their velocity dispersions and use the spherical and projected Jeans equation to obtain the circular velocities. Our findings indicate a nearly isotropic nature for the M31 bulge, while the halo exhibits tangential anisotropy. The results show that the rotation curve remains constant at ∼220 km s−1 up to radius ∼25 kpc and gradually decreases to ∼170 km s−1 further out. Based on the newly determined rotation curve, we have constructed a mass distribution model for M31. Our measurement of the M31 virial mass is $M_{\rm vir} = 1.14^{+0.51}_{-0.35} \times 10^{12} M_\odot$ within rvir = 220 ± 25 kpc.