bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medicamentos potencialmente inapropiados para el adulto mayor en el servicio de medicina interna
Authors: Caviedes Pérez Giovanni , Bobadilla Henao Valentina , Salazar Torres Silvia Piedad , Lozano Garrido Manuela , Pérez Figueroa Jose Manuel , Muñoz Muñoz Diego Armando , Orjuela Camargo Ana María 
Published: October 2023

Introducción: se consideran prescripciones inapropiadas de medicamentos cuando el riesgo supera el beneficio, o en las que hay un incremento en el potencial de interacciones farmacológicas. Para evitarlo en el adulto mayor se han desarrollado herramientas como los criterios Beers y los PRISCUS. Objetivo: identificar las prescripciones potencialmente inapropiadas en la población adulta mayor que consultó al servicio de medicina interna del Hospital Universitario Hernando Moncaleano Perdomo mediante los listados Beers y PRISCUS. Materiales y métodos: estudio observacional descriptivo de corte transversal en pacientes mayores de 65 años, se calculó el tamaño de la muestra en 357 pacientes. La información recolectada fue almacenada en el programa excel versión 2013 y analizada en EPI INFO versión 7.1.4. Resultados: la mediana de edad fue 75 años, el género masculino 52,66%, el promedio de medicamentos por paciente fue de 8,77 con una DE +/- 5. El diagnóstico más frecuente fue insuficiencia cardiaca con 29 casos (8,12 %). La prescripción inapropiada de medicamentos (PIM) según los criterios Beers, se observó en 4,9%, los más frecuentes fueron metoclopramida 20% y amiodarona 14%. La lista PRISCUS identificó 2.5% de prescripciones inapropiadas siendo prazosin (20%) el más relacionado. Conclusiones: los medicamentos encontrados según los criterios BEERS fueron metoclopramida, amiodarona, prazosina, betametildigoxina y con los PRISCUS, prazosina, haloperidol, betametildigoxina y difenhidramina.
Efektivitas Pelayanan Koperasi Tenaga Kerja Bongkar Muat (TKBM) Di Pelabuhan Teluk Nibung Kota Tanjung Balai
Authors: Miftahul Husna Hasibuan  , Tunggul Sihombing  
Published: October 2023

Pelayanan jasa bongkar muat adalah kegiatan usaha yang bergerak dalam bidang bongkar muat barang dari dan ke kapal di pelabuhan. Pekerjaan bongkar muat adalah pekerjaan yang menggunakan fisik untuk mengangkat dan mengangkut barang. Kantor Kesyahabandaraan dan Otoritas Pelabuhan (KSOP) merupakan yang menyelenggarakan kegiatan bongkar muat di wilayah Indonesia salah satunya Pelabuhan Teluk Nibung. Penelitian ini bertujuan untuk melihat efektivitas Koperasi Tenaga Kerja Bongkar Muat dalam bekerja mengangkat dan memuat barang dan mengangkat barang yang dibawa penumpang. Dalam efektivitas yang terkait dalam administrasi publik yaitu cara kerja yang efektif karena pelabuhan ini sudah menjadi pelabuhan Internasional, namun tidak sebesar pelabuhan Belawan, hanya saja pelabuhan Teluk Nibung ini sudah direnofasi menjadi pelabuhan pintu gerbang ke Malaysia. Berdasarkan dari hasil penelitian buruh bongkar/muat pelabuhan di pelabuhan Teluk Nibung mempunyai sistem/aturan kerja yang terdiri dari 2 kelompok kerja yaitu kelompok A dan kelompok B, yaitu kelompok bongkar dan memuat barang dan kelompok memangkat barang yang dibawa penumpang (kargo). Metode yang digunakan dalam penelitian ini adalah kualitatif deskriptif dengan menggunakan teori teori efektivitas yang disampaikan oleh Quinn dan Rorhbaugh. Dalam penelitian ini adalah semua anggota bongkar muat pada KSOP tahun 2019. Hasil penelitian menunjukkan bahwa TKBM dalam Efektivitas Pelayanan Jasa Bongkar Muat di Pelabuhan Teluk Nibung sudah baik meskipun masih ada beberapa kekurangan seperti para usaha bongkar muat barang tidak mengetahui tata cara bekerja dengan terkait kegiatan bongkar muat barang
Validación psicométrica de Pauta de Clasificación de Complejidad Médica de niños, niñas y adolescentes con Necesidades Especiales de Atención en Salud
Authors: Cabezas T. Ana , Rivera L. Carolina , Sarmiento L. Luis 
Published: October 2023

Los niños, niñas y adolescentes con Necesidades Especiales de Atención en Salud (NANEAS), presentan un uso intensivo de recursos por parte de los servicios de salud y demandan un gran nivel de coordinación en la integración de estos servicios, las comunidades y familias. El comité de NANEAS de la SOCHIPE junto a otros expertos generaron una Pauta de Clasificación de Complejidad para otorgar una asignación de recursos adecuada a la necesidad de los casos a través de la Red Asistencial. Objetivo: Realizar una validación de constructo de la Pauta de Clasificación de Complejidad. Sujetos y Método: Con una muestra de 488 niños NANEAS a quienes se aplicó la Pauta de Clasificación, se realizó un análisis de confiabilidad del instrumento, además de un análisis factorial exploratorio y confirmatorio. Resultados: La Pauta de Clasificación obtuvo un Alfa de Cronbach estandarizado de 0,87, sin embargo, los estadísticos de ajuste de modelo RMSEA, TLI y CFI resultaron bajo lo esperado. El coeficiente MI sugirió la eliminación de 9 ítems, a partir de lo cual se generó una Pauta resumida de 11 ítems y tres dimensiones que presentó un RMSEA de 0,60; TLI de 0,970 y CFI de 0,977. Conclusión: El estudio permite contar con una Pauta de Clasificación de Complejidad resumida para niños NANEAS, con buenas propiedades psicométricas, rápida aplicación y fácil interpretación, para la aplicación en la Red Asistencial de Salud.
The Characteristic of Sticks Mung Bean Flour (Vigra radiate L.) and The Addition of Celery (Apium graveolens)
Authors: Sakura Hida Arum , Suhartatik Nanik , Karyantina Merkuria 
Published: October 2023

Sticks are delicious, crunchy, savory, long, thin, and brownish yellow snacks with various flavors. The main ingredients of this product consist of wheat flour, , eggs, garlic, pepper, and salt. Celery leaves were chosen because they are easy to obtain and to optimize local ingredients. Mung bean (Phaseolus radiates) itself is a vegetable food that is high in protein and fiber where the protein contained is 20-25%. This study aims to determine the effect of sticks mung bean flour and the addition of celery. The design used was a 2 factor factorial completely randomized design (CRD). Factor 1 is the ratio of wheat flour : mung bean flour (75:25, 50:50, 25:75) and factor 2 is the concentration of celery porridge (10%, 20%, 30% in 30 ml of water). The best results of chemical analysis based on the highest protein content were the ratio of wheat flour : mung bean flour 25:75 with concentration of celery porridge 20% with 2.22% water content, 1.74% ash content, 23.58% fat content, 17.75% protein content, 8.28% crude fiber and 46.41 for carbohydrates by difference, color sensory test value 3.73 (greenish brown), crunchy texture 3.40 (crispy), celery taste 3.60 (taste celery flavor) mung bean taste 2.67 (a bit of green bean flavor), celery aroma 3, 33 (smelling of celery), and 3.27 (preferred) for overall liking. The most preferred formulation of celery sticks by consumers is the comparison of wheat flour : mung bean flour 75:25 with the addition of 10% celery porridge concentration with a value of 3.47 (preferred). Sticks made from green beans and celery can be an alternative food that is high in protein and fiber.
Physical and Sensory Characteristics of Gluten Free Cookies Prepared from Black Glutinous Rice and MOCAF Flour Combination
Authors: Putri Destiana Adinda , Komalasari Husnita , Ulandari Devy , Isnain Fuad Sauqi 
Published: October 2023

Extensive research has been conducted to investigate the formulation of a new generation of cereal-based Gluten Free (GF) foods formulated with High-Resistant Starch (RS) sources and high in nutritional value. Black glutinous rice flour is a rich in bioactive compound and MOCAF is known for its high RS content often used as an alternative to wheat flour in the production of gluten-free cookies. The formulation of the combination of these two ingredients needs to be investigated to produce gluten free cookies that have good physical and sensory characteristics. The aims of these research was to investigate the effect of different ratio of Black Glutinous Rice Flour (BGRF) and MOCAF (MF) formulation (F1 = 100:0, F2= 75:25, F3=50:50, F4= 25:75, F5= 0:100) on physical and sensory parameter of resultant GF cookies. The results shows that, cookies with high amount of BGRF showed interesting physical properties with higher diameter, spread ratio, and ∆E of the cookies, lower weight, thickness, baking loss, L*, a*, b*, C* value, and the hedonic score of color, aroma, taste, texture, overall compared to cookies with 100% MF. The best formula that produces gluten free cookies with the highest taste score score is F4 (BGRF 25%:MF 75%) with physical characteristics weight 10.70 g, diameter 48.40 mm, thickness 8.60 mm, spread ratio 5.63, baking loss 17.37 %, L* 53.96, a* 0.63, b* 17.65, C* 17.66, ∆E 48.78. With the combination of BGRF and MF on cookies dough, we manage to obtain cookies that has great potential to increase the nutritional quality of gluten-free food.
SAFETY AND EFFICACY OF SEMI-RIGID URETEROSCOPE IN RETROGRADE INTRARENAL SURGERY FOR RENAL PELVIC STONES UPTO 2 CM
Authors: Khattak Ikramullah , Khan Riaz Ahmad , Izhar Muhammad , Khan Mazhar , Khan Aslam , Jehan Shah 
Published: October 2023

Objectives: To determine the efficacy and safety of RIRS with rigid URS for renal pelvis stone between 1 to 2cm.
Materials and Methods: This is a prospective observational case series of 48 patients who under RIRS at Institute of Kidney Diseases, Peshawar during 1st January 2022 and 31st December The primary indication was single renal pelvic calculi of up to 2 cm diameter in patients with previously failed treatment modalities or as primary cases. Data was analysed regarding patient demographics, stone size, surgery time, complications and clearance rates within a 2 months follow-up period.
Results: Total of Forty-eight patients with 29 (60.4%) males and 19 (39.6%) females in a ratio of 1.5 to 1. The mean age was 38.7 ± 10.8 years (range: 19 – 60 years). Mean stone diameter was 15.3 ± 2.6 mm. Early postoperative complications included fever, haematuria and retained stones. There were 12 (25%) cases of postoperative fever who resolved without any major sequelae, and 11 (22.9%) cases of early postoperative haematuria of which 4 (8.3%) patients received blood transfusions. The overall clearance rate after fi rst RIRS was 87.5% (n = 42) with a P value of 0.01 and is clinically signifi cant. Six (12.5%) patients were found to have stone fragments retained. The clearance rate after 2nd look RIRS was 95.83% (n = 46).
Conclusion: Retrograde intrarenal surgery using semi-rigid ureteroscope for renal pelvic stones has favourable stone free rates and the results are comparable to percutaneous nephrolithotripsy. Complications requiring intervention are below ten percent and are non-fatal.
A Systematic Review and Meta-analysis of Injecting Drug Use as a Risk Factor of HIV in Bangladesh
Authors: Chowdhury Swarna , Akter Noor Jahan , Rahman M Shafiqur , Saha Priom , Barman Prianka , Fahin Tasmin Akter , Roy Swapnil , Dey Durjoy , Hassan Md Mehedi , Munmun Tasnim Sultana , Ashrafi Samia , Rahman Maisha Maliha 
Published: October 2023

HIV is a new, transpiring problem, especially, among injecting drug users (IDU) in Bangladesh. Although HIV prevalence in Bangladesh compared to the neighboring countries is still low, ongoing high-risk behaviors among injecting drug users could facilitate the more extensive transmission of HIV to the general population. This study aims to assess the relationship between HIV and injecting drug use. Through a systematic review, we included 19 studies that met the inclusion/exclusion criteria. Pooled prevalence, odds ratio, 95% CI, and measures of heterogeneity were calculated by the random-effects model. Publication bias was examined by funnel plots and Egger‘s test. We found the overall pooled prevalence was 3.65% (95% CI: 2.10-5.56%) which indicates that Bangladesh is on the brink of a concentrated epidemic. IDUs were 6.085 times more likely to be HIV-positive than non-IDUs (pooled OR: 6.085; 95% CI: 4.654-7.956). The pooled prevalence for males was reported to be 2.44% (95% CI: 1.51-3.57%), which was significantly higher than those for females with a pooled prevalence of 0.26% (95% CI: 0.00-1.19%). This meta-analysis revealed that injecting drug use is a significant risk factor for HIV in Bangladesh. Moreover, while the HIV prevalence in female IDUs was found to be very low, it was much higher in male IDUs.
Dhaka Univ. J. Sci. 71(2): 160-168, 2023 (July)
PENGARUH KONSELING BEHAVIOR TERHADAP KECEMASAN BELAJAR PADA SISWA DI SMAN 1 LABUAPI
Authors: Astuti Farida Herna , Mustakim Mustakim 
Published: October 2023

Peran orang tua pada anak yang mengalami kecemasan belajar yaitu melatih anak-anaknya untuk tetap percaya diri dan membentuk mental yang kuat. Peran orang tua untuk memperhatikan dan membimbing anaknya adalah untuk mendukung kebutuhan mental dan spiritual anak. dengan cara bertanggung jawab mencari nafkah dan membimbing anak-anaknya yang terkait. Tujuan dalam penelitian ini adalah untuk mengetahui “Pengaruh konseling Behavior Terhadap Kecemasan Belajar Pada Siswa Kelas XI di SMAN 1 Labuapi Tahun Pelajaran 2022/2023. Jumah Populasi dalam penelitian ini adalah 41 sedangkan sampelmya adalah 8 dengan metode pengumpulan data yang digunakan yaitu angket sebagai metode pokok, observasi dan dokumentasi dan wawancara sebagai metode pelengkap. Teknik analisis data yang digunakan yaitu dengan menggunakan rumus t test. Sesuai dengan analisis data yang digunakan yaitu metode statistik dengan menggunakan rumus t-tes. Berdasarkan hasil penelitian diperoleh Ada Pengaruh Konseling Behavior Terhadap Kecemasan Belajar Pada Siswa Kelas XI di SMAN 1 Labuapi. Pengaruh tersebut dapat dilihat dari adanya perbedaan nilai antara setelah siswa mengikuti Konseliang kelompok (pos-test) dengan sebelum siswa mengikuti Konseling kelompok (pre-tes). Dari hasil analisis data: nilai t-test setelah diperoleh t-hitung = sedangkan t-tabel dengan taraf signifikan 5% pada db (N-1) = 8 – 1 = 7 menunjukkan t-tabel = 2,365 ini menunjukkan bahwa nilai (t-hitung > t-tabel) yaitu (> 2,365). Dengan demikian hipotesis alternatif (Ha) diterima sedangkan hipotesis nol (Ho) ditolak.
Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae
Authors: Feng Qing , Ning Xiaoyu , Qin Lei , Li Jun , Li Chun 
Published: October 2023

Introduction: Both CRISPR/dCas9 and CRISPR/dCpf1 genome editing systems have shown exciting promises in modulating yeast cell metabolic pathways. However, each system has its deficiencies to overcome. In this study, to achieve a compensatory effect, we successfully constructed a dual functional CRISPR activation/inhibition (CRISPRa/i) system based on Sp-dCas9 and Fn-dCpf1 proteins, along with their corresponding complementary RNAs.Methods: We validated the high orthogonality and precise quantity targeting of selected yeast promoters. Various activating effector proteins (VP64, p65, Rta, and VP64-p65-Rta) and inhibiting effector proteins (KRAB, MeCP2, and KRAB-MeCP2), along with RNA scaffolds of MS2, PP7 and crRNA arrays were implemented in different combinations to investigate quantitative promoter strength. In the CRISPR/dCas9 system, the regulation rate ranged from 81.9% suppression to 627% activation in the mCherry gene reporter system. Studies on crRNA point mutations and crRNA arrays were conducted in the CRISPR/dCpf1 system, with the highest transcriptional inhibitory rate reaching up to 530% higher than the control. Furthermore, the orthogonal CRISPR/dCas9-dCpf1 inhibition system displayed distinct dual functions, simultaneously regulating the mCherry gene by dCas9/gRNA (54.6% efficiency) and eGFP gene by dCpf1/crRNA (62.4% efficiency) without signal crosstalk.Results and discussion: Finally, we established an engineered yeast cell factory for β-carotene production using the CRISPR/dCas9-dCpf1 bifunctional system to achieve targeted modulation of both heterologous and endogenous metabolic pathways in Saccharomyces cerevisiae. The system includes an activation module of CRISPRa/dCas9 corresponding to a gRNA-protein complex library of 136 plasmids, and an inhibition module of CRISPRi/dCpf1 corresponding to a small crRNA array library. Results show that this CRISPR/dCas9-dCpf1 bifunctional orthogonal system is more quantitatively effective and expandable for simultaneous CRISPRa/i network control compared to single-guide edition, demonstrating higher potential of future application in yeast biotechnology.
Expanding flavone and flavonol production capabilities in Escherichia coli
Authors: Yiakoumetti Andrew , Hanko Erik K. R. , Zou Yutong , Chua Jeremy , Chromy Jakub , Stoney Ruth A. , Valdehuesa Kris Niño G. , Connolly Jack A. , Yan Cunyu , Hollywood Katherine A. , Takano Eriko , Breitling Rainer 
Published: October 2023

Flavones and flavonols are important classes of flavonoids with nutraceutical and pharmacological value, and their production by fermentation with recombinant microorganisms promises to be a scalable and economically favorable alternative to extraction from plant sources. Flavones and flavonols have been produced recombinantly in a number of microorganisms, withSaccharomyces cerevisiaetypically being a preferred production host for these compounds due to higher yields and titers of precursor compounds, as well as generally improved ability to functionally express cytochrome P450 enzymes without requiring modification to improve their solubility. Recently, a rapid prototyping platform has been developed for high-value compounds inE. coli, and a number of gatekeeper (2S)-flavanones, from which flavones and flavonols can be derived, have been produced to high titers inE. coliusing this platform. In this study, we extended these metabolic pathways using the previously reported platform to produce apigenin, chrysin, luteolin and kaempferol from the gatekeeper flavonoids naringenin, pinocembrin and eriodictyol by the expression of either type-I flavone synthases (FNS-I) or type-II flavone synthases (FNS-II) for flavone biosynthesis, and by the expression of flavanone 3-dioxygenases (F3H) and flavonol synthases (FLS) for the production of the flavonol kaempferol. In our best-performing strains, titers of apigenin and kaempferol reached 128 mg L−1and 151 mg L−1in 96-DeepWell plates in cultures supplemented with an additional 3 mM tyrosine, though titers for chrysin (6.8 mg L−1) from phenylalanine, and luteolin (5.0 mg L−1) from caffeic acid were considerably lower. In strains with upregulated tyrosine production, apigenin and kaempferol titers reached 80.2 mg L−1and 42.4 mg L−1respectively, without the further supplementation of tyrosine beyond the amount present in the rich medium. Notably, the highest apigenin, chrysin and luteolin titers were achieved with FNS-II enzymes, suggesting that cytochrome P450s can show competitive performance compared with non-cytochrome P450 enzymes in prokaryotes for the production of flavones.
STAT1 deficiency underlies a proinflammatory imprint of naive CD4+ T cells in spondyloarthritis
Authors: Cherqaoui Bilade , Crémazy Frédéric , Lauraine Marc , Shammas Ghazal , Said-Nahal Roula , Mambu Mambueni Hendrick , Costantino Félicie , Fourmont Marine , Hulot Audrey , Garchon Henri-Jean , Glatigny Simon , Araujo Luiza M. , Breban Maxime 
Published: October 2023

IntroductionIn spondyloarthritis (SpA), an increased type 3 immune response, including T helper cells (Th) 17 excess, is observed in both human and SpA animal models, such as the HLA-B27/human β2-microglobulin transgenic rat (B27-rat).MethodsTo investigate this unexplained Th17-biased differentiation, we focused on understanding the immunobiology of B27-rat naive CD4+ T cells (Tn). ResultsWe observed that neutrally stimulated B27-rat Tn developed heightened Th17 profile even before disease onset, suggesting an intrinsic proinflammatory predisposition. In parallel with this observation, transcriptomic and epigenomic analyses showed that B27-rat Tn exhibited a decreased expression of Interferon/Th1- and increased expression of Th17-related genes. This molecular signature was predicted to be related to an imbalance of STAT1/STAT3 transcription factors activity. Stat1 mRNA and STAT1 protein expression were decreased before disease onset in Tn, even in their thymic precursors, whereas Stat3/STAT3 expression increased upon disease establishment. Confirming the relevance of these results, STAT1 mRNA expression was also decreased in Tn from SpA patients, as compared with healthy controls and rheumatoid arthritis patients. Finally, stimulation of B27-rat Tn with a selective STAT1 activator abolished this preferential IL-17A expression, suggesting that STAT1-altered activity in B27-rats allows Th17 differentiation. DiscussionAltogether, B27-rat Tn harbor a STAT1 deficiency preceding disease onset, which may occur during their thymic differentiation, secondarily associated with a persistent Th17 bias, which is imprinted at the epigenomic level. This early molecular phenomenon might lead to the persistent proinflammatory skew of CD4+ T cells in SpA patients, thus offering new clues to better understand and treat SpA.
Causal relationship between spondylarthritis and stroke in a European population: a two sample Mendelian randomization study
Authors: Zhang Luofei , Yu Kefu , Huo Jiping , Mei Shenghui , Zhao Zhigang , Zhu Bin 
Published: October 2023

BackgroundObservational studies have found an increased risk of stroke in patients with spondyloarthritis, but the results are susceptible to reverse causality and confounders. Therefore, the study aimed to further explore the association between spondyloarthritis and different subtypes of stroke by using a two sample Mendelian randomization (MR) analysis.MethodsGenetic instrumental variables for spondyloarthritis were identified using summary level data from a genome-wide association study involving 201,581 people. Summary statistics from the Multiancestry Genome-wide Association Study of Stroke Consortium were used to obtain genetic data on stroke. There was no sample overlap between the exposure and outcome datasets. Inverse-variance weighted was considered the primary MR method for causal analysis. Heterogeneity, pleiotropy and sensitivity analyses were performed to ensure robustness, and single nucleotide polymorphism (SNP) with potential confounders was further screened in the PhenoScanner database to better evaluate the stability of our study.ResultsOne SNP (rs1065045) was excluded due to schizophrenia. After excluding SNP (rs1065045), results of the second MR analysis were slightly different from the first, which were considered as the final result: a significant positive causality between spondyloarthritis and cardioembolic stroke (OR=1.296, 95% CI:1.094-1.534, p=0.003); a possible positive causality between spondyloarthritis and any stroke (OR=1.082, 95% CI:1.016-1.152, p=0.013)/any ischemic stroke (OR=1.086, 95% CI:1.013-1.163, p=0.020); no significant/possible causality between spondyloarthritis and small vessel stroke (OR=1.168, 95% CI:0.993-1.375, p=0.061). Insufficient power may be one possible reason why a causality was not observed between spondyloarthritis in our study.ConclusionsThis study suggests that the possible causative effects of spondyloarthritis predicted by genetics on stroke may be limited to any stroke, any ischemic stroke, and cardioembolic stroke, especially the last.