bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCNA independence of Ki‐67 expression in HPV infection.
Authors: Boon M. E. , Howard C. V. , van Velzen D. 
Published: October 2002

Abstract12 Infectious Wart lesions were stained using the streptavidin‐biotin immunoperoxidase method for PCNA10, MIB‐1 (Ki‐67 equivalent antigen) and Human Papilloma Virus antigen to study the effect of HPV presence on epidermal proliferation. Using strict methods to avoid observer bias, Ki‐67 antigen was found in a high proportion of nuclei in the suprabasal layers together with HPV antigens, in the absence of PCNA staining. This finding indicates that DNA synthesis related, Ki‐67 antigen bearing structures can be raised in the human nucleus in the absence of induction of PCNA bearing structures, suggesting also structural independence between the antigen bearing molecules.
Steady‐state levels of different tenascin mRNAs in various normal human tissues.
Authors: Borsi Laura , Carnemolla Barbara , Ponassi Marco , Zardi Luciano 
Published: October 2002

AbstractNorthern blot analysis of TN mRNA from different human tissues shows two major bands of about 6 and 8 kb which correspond to two different mRNAs generated by alternative splicing of the primary transcript. In liver, pancreas and kidney only the 6 kb TN mRNA was detectable. The highest levels of 8 kb TN mRNA were observed in placenta and skin representing 30% and 52% of total TN mRNA, respectively. In all other tissues tested the 8 kb TN mRNA represented less than 20% of total TN mRNA.
Phenotype of T cell clones obtained from bronchial biopsies and peripheral blood from three asthmatics.
Authors: Pène Jérôme , Lagier Béatrice , Rivier Agnès , Chanez Pascal , Vendrell Jean‐Pierre , Bousquet Jean 
Published: October 2002

AbstractEstablishment of T cell clones derived from bronchial biopsies and peripheral blood from allergic asthmatics has shown that they secret a different IL‐4 and IFN‐γ pattern of cytokines, suggesting that the micro‐environment of the bronchi may influence the phenotype of the T cells.
Effects of catechins on the mouse lung carcinoma cell adhesion to the endothelial cells.
Authors: Isemura Mamoru , Suzuki Yasuo , Satoh Ken , Narumi Koh , Motomiya Masakichi 
Published: October 2002

AbstractWe studied the effects of 5 kinds of catechins on the adhesion of mouse lung carcinoma 3LL cells to the monolayer of bovine lung endothelial cells. (‐)‐Epicatechin gallate and (‐)‐epigallocatechin gallate were active in inhibiting the 3LL cell adhesion, while (+)‐catechin and (‐)‐epicatechin were inactive. (‐)‐Epigallocatechin showed a considerable cytotoxicity. These data suggest that the specific chemical structure is required to exert the inhibitory activity of catechins and the search for the cellular binding protein(s) bound to these inhibitory catechins would provide a clue to clarify the mechanism of interactions between tumor cells and endothelial cells.
N‐acetyl‐cysteine enhances cell adhesion properties of epithelial and lymphoid cells.
Authors: Rivabene Roberto , Viora Marina , Matarrese Paola , Rainaldi Gabriella , D'Ambrosio Antonella , Malorni Walter 
Published: October 2002

AbstractIn this paper, we show that the antioxidant N‐acetyl‐cysteine (NAC) is capable of enhancing the adhesion properties of the epithelial cell line A431 and of the lymphocytic cells with cytotoxic activitv from human peripheral blood: the natural killer (NK) cells. This effect leads to an increased efficiency of A431 cells to form a monolayer and of NK cells to kill their targets. In both cases a specific effect of NAC was found in the distribution of those molecules of the cytoskeleton which are generally involved in cell substrate and cell‐to‐cell contact region formation, e.g., the actin microfilaments. NAC could thus behave as a drug influencing certain cytoskeleton‐dependent cell processes in a non‐histotype dependent manner.
PHOSPHOLIPIDS AND NUCLEAR RNA
Authors: ALBI E. , MICHELI M. , MAGNI VIOLA M. P. 
Published: October 2002

AbstractIt has been demonstrated that in hepatocyte nuclei the chromatin phospholipid fraction is localized near the RNA in decondensed chromatin. The aim of the present study was to see if there is any linkage between phospholipids and other nuclear components. Isolated hepatocyte nuclei and nuclear membranes were treated with deoxyribonuclease and ribonuclease. No loss of phospholipids was observed after DNA digestion, whereas 48% was lost following enzymatic RNA removal. This loss of phospholipids, localized either near the membrane or inside the nucleus, was not homogeneous for all phospholipids: phosphatidylserine and sphingomyelin being the most affected. It can be concluded that 48% of nuclear phospholipids, in particular sphingomyelin, is lost with RNA removal. This result is discussed in view of a possible role of phospholipids in DNA synthesis and RNA transcription.
INHIBITION OF CELLULAR GROWTH AND STEROID 11β‐HYDROXYLATION INRAS‐TRANSFORMED ADRENOCORTICAL CELLS BY THE FUNGAL TOXINS BETICOLINS
Authors: DING GUOQING , MAUME GABRIELLE , MILAT MARIE‐LOUISE , HUMBERT CLAUDE , BLEIN JEAN‐PIERRE , MAUME BERNARD F. 
Published: October 2002

AbstractThe proliferation of GM16 and 4CDTras‐transformed newborn rat adrenocortical (RTAC) cells and Y1 mouse adrenal tumor cells was inhibited by beticolins, the fungal toxins extracted fromCercospora beticola, at submicromolar concentrations in a dose‐dependent manner. Inhibitory concentrations for half the maximum inhibition were 150, 75 and 25 nmfor beticolin‐1 and 230, 150 and 50 nmfor beticolin‐2 in GM16, 4CDT and Y1 cells respectively. Beticolins strongly inhibited the production of 11β‐hydroxysteroids on the second and third days of treatment in a dose‐dependent manner between 0.1 and 1 μm. Beticolins were shown by confocal microscopy to be localized in cytoplasmic organelles about 30–40 min after treatment. This finding favors a direct action of beticolins on mitochondrial steroid 11β‐hydroxylase albeit another less direct mechanism involving a cytoplasmic signaling pathway cannot be excluded.
HUMAN B CELL DIFFERENTIATION: DEPENDENCE ON INTERACTIONS WITH MONOCYTES AND T LYMPHOCYTES VIA CD40, CD80 (B7.1), AND THE CD2‐LIGANDS CD48 AND CD58 (LFA‐3)
Authors: HOFFMANN JÖRG C , KRÜGER HARTMUT , ZIELEN STEFAN , BAYER BETTINA , ZEIDLER HENNING 
Published: October 2002

AbstractB cell differentiation depends on cellular interactions with T lymphocytes and monocytes via adhesion molecules (AM). In order to characterize AM which are required for B cell differentiation immunoglobulin production using unseparated peripheral blood mononuclear cells (PBMC) was studied. Unstimulated human PBMC were cultured for 9 days with mAb directed at CD2/CD48, /CD58, CD59, CD5/CD72, CD11a—CD18/CD54, CD28/CD80, CD86, CD40/CD40L, or rat CD2 (control). B cell differentiation was quantified measuring IgM and in some cases IgA, IgG, and IgE production. IgM levels were significantly reduced by mAb against CD40, CD48, CD58 and CD80. The reduction was not due to isotype switching to IgA, IgG or IgE. The role of CD40, CD48, CD58 and CD80 was further investigated after depletion of different cell types. Depletion of monocytes and NK cells resulted in no detectable IgM production irrespective of added mAbs. In contrast, IgM production was still present after depletion of T cells and NK cells. Only mAb against CD80 and CD48 significantly reduced IgM production, the reduction of IgM production by anti‐CD40 mAb was less than in the presence of T cells. Importantly, anti‐CD58 mAb had no effect on IgM production after T cell and NK cell depletion. Taken together, the AM CD40, CD48, CD58, and CD80 are involved in Ig production of unseparated PBMCs. In this model of B cell differentiation only the AM CD58 depend on the presence of T cells while CD48 and CD80 help was found to be T cell independent.
G2RESTRICTION POINT WITHIN POPULATIONS OF HEPATOCYTES AND TONGUE KERATINOCYTES IN YOUNG, GROWING MICE
Authors: ERRECALDE ANA L. , GARCÍA ADRIANA L. , INDA ANA M. , BADRÁN AMADO F. 
Published: October 2002

AbstractThe authors studied the effect of either extracts from liver (LE) or the malignant tumour ES2 (TE) or plasma from intact mice (PI) or tumour‐bearing animals (PT) on the mitotic activity of the hepatocytes and tongue keratinocytes in young, growing C3H/s male mice (28±1 days old). Animals standardized for periodicity analysis were injected intraperitoneally with either TE, LE, PI, PT, or saline (S) at 16:00h with 0.01ml of sample/g of body weight and were then killed at (time of day/h post‐injection) 20:00/04, 00:00/08, and 04:00/12. Colchicine (2μg/g) was injected 4h before death. Samples of the liver and tongue from each animal were processed for histology and assessment of mitotic index. The results were expressed as colchicine‐arrested metaphases/1000 nuclei. The TE and LE stimulated the mitotic activity of hepatocytes and tongue keratinocytes. Taking into account the time elapsed between the injections and the measurements made in these light‐dark synchronized animals, we conclude that the increase in mitotic index observed in those tissues stemmed from a reinitiation of cell‐cycle traverse in a subpopulation of G2‐arrested, noncycling cells.
MODIFICATION OF GLYCOSYLATION REDUCES MICROVILLI ON RAT LIVER EPITHELIAL CELLS
Authors: Zhao F. T. , Li J. , Shi G. X. , Liu Y. , Zhu L. P. 
Published: October 2002

AbstractEffect of glycosylation modification on the shape of microvillus was investigated on rat liver epithelial cell line WB‐F344. Since rat ER α‐mannosidase has 89% identity to human 6A8 α‐mannosidase, we used an antisense 6A8 cDNA fragment to inhibit expression in WB‐F344 cells. Cells were transfected with antisense 6A8, the relevant sense fragment or the mock plasmid. Genomic PCR for neoR demonstrated integration of the transfected gene into host DNA. Enzymatic activity assay on p ‐nitro‐phenyl‐α‐D‐mannopyranoside showed suppression of ER α‐mannosidase expression in the cells transfected with antisense 6A8. Concanavalin A binding to these cells was enhanced, indicating a modification in glycosylation. Number reduction and blunting of microvillus on these cells was observed. Transduction with the sense fragment or the mock had no effect. Cells with suppressed ER α‐mannosidase expression grew slower in culture. Our results indicate an obvious effect of glycosylation modification on microvilli, which might be related with malfunctioning of cells.
EXAMINATION OF ACTIN POLYMERIZATION AND VISCOSITY INDUCED BY CATIONS AND IONIC STRENGTH WHEN CROSS‐LINKED BY α‐ACTININ
Authors: Goldmann Wolfgang H. 
Published: October 2002

AbstractIncreasing potassium chloride concentration from 0 to 100mM and magnesium chloride from 0 to 2mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2mM decreases the rate of polymerizing actin. The presence of α‐actinin has little influence on the polymerization kinetics of actin under these conditions. Viscometric measurements indicate that the presence of various mono‐ and divalent cations, ionic strength, and α‐actinin in combination are responsible for changes in the mechanical properties of solutions containing actin. The actin filament dynamic behavior is drastically reduced under these conditions as confirmed by quasi‐elastic light scattering.
Implications of Peat Accumulation at Point Escuminac, New Brunswick
Authors: Warner Barry G. , Clymo Richard S. , Tolonen Kimmo 
Published: October 2002

AbstractThe world's peatlands contain about 450 GT of readily decomposeable organic carbon. Peat-forming systems have two main layers, of which the lowest is the thickest and includes the peat proper. The upper layer fixes carbon by photosynthesis, loses it by selective decay, and passes on about 15% to the lower zone; here decay continues, although very slowly. One consequence is that as for Point Escuminac, New Brunswick, the relation of age to depth may be concave. Although the surface of the peatland is as productive as ever, the true rate of carbon accumulation is decreasing; after 10,000 yr it is only 33% as efficient at sequestering carbon as it was when the peatland began to grow. Peatlands are usually thought to represent sinks for carbon, but a warming climate could make some peatlands carbon sources rather than sinks, thus initiating positive feedback.
Relationship of Temperature and Light Ring Formation at Subarctic Treeline and Implications for Climate Reconstruction
Authors: Yamaguchi David K. , Filion Louise , Savage Melissa 
Published: October 2002

AbstractDuring the past 8 centuries, light rings (LRs) have occasionally formed in black spruce (Picea mariana) at treeline near Bush Lake, northern Quebec (L. Filion, S. Payette, L. Gauthier, and Y. Boutin, 1986, Quaternary Research 26, 272-279; A. Delwaide, L. Filion, and S. Fayette, 1991, Canadian Journal of Forest Research 21, 1828-1832). New analyses of climate data compiled during the period of overlapping tree-ring and instrumental records show that years of LR formation at Bush Lake have unusually cool May, June, August, and September temperatures. The analyses also show that August-September temperatures strongly correlate with May-July temperatures. Thus, late spring and entire growing-season temperatures influence LR formation at subarctic treeline. LRs formed in at least 5% of the trees at Bush Lake when May-September mean temperatures at Inukjuak fell below 4.2°C and August-September mean temperatures fell below 6.7°C. These threshold temperature/LR relationships can be used to infer limiting summer temperatures during the period preceding instrumental records. For example, the LR record suggests that May-September temperatures at northern Quebec treeline dropped below 4.2°C in A.D. 1601 after a major volcanic eruption of unknown source. Visual assessments of LR occurrence provide a new approach for extracting quantitative paleoclimatic information from tree rings.
New Geochemical Evidence for the Youngest Toba Tuff in India
Authors: Shane Phil , Westgate John , Williams Martin , Korisettar Ravi 
Published: October 2002

AbstractNew geochemical data on tephra samples from a layer present at several archeological sites in India support correlation of this layer to the Youngest Toba Tuff, erupted from northern Sumatra about 74,000 yr ago. The data show that the Indian tephra layer is not a correlative of older tephra erupted from Toba, as has been suggested on the basis of artifact assemblages. Previously published geochemical data on the Indian tephra beds was based on bulk ash samples containing mineral and clay contaminants, and the resulting variability in analyses did not allow identification or discrimination of individual eruptive events. Our new data were collected on individual glass shards and small, purified glass separates which have greater resolving power in fingerprinting. Acheulian and Paleolithic artifacts found at some of the Indian tephra sites do not reflect the antiquity of the tephra bed, as they occur in fluvial sediments and may be reworked.
An Alluvial Surface Chronology Based on Cosmogenic36Cl Dating, Ajo Mountains (Organ Pipe Cactus National Monument), Southern Arizona
Authors: Liu Beiling , Phillips Fred M. , Pohl Molly M. , Sharma Pankaj 
Published: October 2002

AbstractA chronology of alluvial surfaces on piedmont slopes below the western Ajo Mountains, southern Arizona, has been obtained using cosmogenic36Cl accumulation and AMS radiocarbon dating. The apparent36Cl ages of individual boulders range from 520,000 to 13,000 yr, and the14C ages of organic material in the two young terraces are 2750–2350 and 17,800 cal yr B.P. The sequence of36Cl ages is consistent with the apparent stratigraphic order, but groupings of similar ages for different surfaces appear to result from repeated reworking of older surfaces associated with the deposition of younger ones. The youngest surface gave a distribution of36Cl ages about 30,000 yr older than the14C and soil ages; however, this distribution had36Cl ages that overlapped with36Cl ages from active channels and hillslopes. We attribute the older-than-expected exposure ages of sampled boulders to inheritance of36Cl while residing near the surface during very slow erosion on the mountain front. Our results show that although cosmogenic nuclide accumulation can help establish chronologies for surfaces in piedmont settings, care must be used in evaluating the effects of complex exposure histories.
Fossil Beetle Evidence for a Short Warm Interval near 40,000 yr B.P. at Titusville, Pennsylvania
Authors: Cong Shaoguang , Ashworth Allan C. , Schwert Donald P. , Totten Stanley M. 
Published: October 2002

AbstractA brief cold–warm–cold climate change during the middle Wisconsinan is described for the first time in North America, based on fossil beetle assemblages at Titusville, Pennsylvania. AMS dating of insect chitin and wood suggests the change occurred between 39,000 and 43,500 yr B.P. Basal peats in river terrace deposits contain arctic–subarctic beetle species representing a climate similar to that found at treeline in Canada, where mean July temperatures range from 10° to 13°C. These cold-adapted beetles were replaced by species representing a climate similar to the mixed coniferous–deciduous forests of southern Canada, where mean July temperatures range from 18° to 20°C. In turn, these warmer-adapted beetles were replaced by arctic–subarctic species in the upper part of the section, representing a climate with mean July temperatures in the range 10°–13°C. The brief warm interval is informally designated the Titusville interstade. The difference in temperature between the colder and warmer intervals is about 5°–7°C. The Titusville interstade is correlated with the Upton Warren Interstade of the British Isles which is of similar age and intensity. It is also correlated with the high sea-surface temperature interval between Heinrich events 4 and 5 represented in North Atlantic sediment cores.
Anatomie resection for severe blunt liver trauma in 100 patients: Significant differences between young and elderly
Authors: Tsugawa Kouji , Koyanagi Nobuhiro , Hashizume Makoto , Ayukawa Katsuhiko , Wada Hiroya , Tomikawa Morimasa , Ueyama Toshihiko , Sugimachi Keizo 
Published: October 2002

AbstractThe liver is the organ most commonly injured during blunt abdominal trauma. As our society ages, emergency surgery for active elderly patients increases, but data on aggressive emergency hepatic resection remain scarce in the literature. The purpose of this study was to determine whether the elderly (70 years of age or older) can tolerate major liver injury and subsequent hepatic resection. We investigated 100 patients who were treated by an anatomic resection for severe blunt liver trauma (29 elderly patients who were 70 years of age or older and 71 young patients who were younger than 70 years of age) in a retrospective study. The elderly patients were more severely injured as demonstrated by a higher Injury Severity Score, a lower Glascow Coma Scale, and lower survival (80.3% vs. 65.5%; p<0.05). The total number of associated injuries was greater in elderly patients. Motor vehicle accidents were responsible for 71.8% of the injuries in the young group, and the predominant mechanism in the elderly patients was also motor vehicle accidents (51.7%). The 71 anatomic hepatic resections performed on the young patients included right hemihepatectomy (n=45), left lateral segment resection (n=14), bisegmentectomy (n=5), and others. The 29 anatomic hepatic resections performed for the elderly patients were right hemihepatectomy (n=15), left lateral segment resection (n=5), left hemihepatectomy (n=4), and others. Pneumonia, subphrenic abscess, and urosepsis occurred at a significantly higher frequency in elderly patients than in young patients. Our data clearly indicated that (1) the mechanism of injury, grade of associated intraabdominal injuries, distribution of surgical procedures, and complications differ significantly between young and elderly patients; and (2) the survival rate (65.5%) in elderly patients may be sufficient to consider anatomic hepatic resection to be a useful, safe procedure.
Investigative modalities for massive lower gastrointestinal bleeding
Authors: Al Qahtani Aayad R. , Satin Richard , Stern Jerry , Gordon Philip H. 
Published: October 2002

AbstractThe objective of this study was to evaluate the efficacy of various diagnostic modalities in the assessment of patients with massive lower gastrointestinal bleeding. The charts of all patients admitted to a McGill University affiliated teaching hospital with the diagnosis of lower gastrointestinal bleeding over a 25‐year period were reviewed. There were 136 patients who underwent 202 admissions. The information documented included demographics on age, gender, co‐morbid disease, prescribed medications, requirements for blood transfusions, orthostatic change in blood pressure, acute drop in hematocrit (to <30%), and exclusion of upper gastrointestinal bleeding. Among the 202 admitted patients there were 116 men and 86 women), with an average age of 70 years (range 16–95 years). At least one significant medical disease was found in 93% of these patients; and 20% were on aspirin and 5% on anticoagulants at the time of diagnosis. Rigid or flexible sigmoidoscopy was performed in 68 and 18 patients, respectively, with a definitive diagnosis made in 2.9% and 11.0%, respectively. Colonoscopy was performed in 152 cases, 20 of which were incomplete; a specific diagnosis was made for 59 admissions (45%). A red blood cell or colloid scan was performed on 53 patients, with extravasation noted in 13 (24.5%); a localized site of bleeding was identified in 9 cases (17%). Angiography was performed on 31 patients with bleeding sites localized in 6 (19%). Barium enemas were completed in 85 of 92 patients, and the presumptive cause of bleeding was identified in 72% of those with a complete examination. The most common causes identified were diverticulosis in 52 patients and angiodysplasia in 14. The cause of bleeding was not detected in 48 (35%). Bleeding stopped in most patients spontaneously, with only 7 requiring operation. The average number of units transfused was 3 (range 0–26). Scintigraphy and angiography were less efficacious than colonoscopy for localizing the site and etiology of the bleeding. Despite the combination of investigative modalities, a definitive diagnosis was not made in 35% of the admitted patients. The need for operative intervention in our study was lower than in most previous reports.