bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioadhesive Microcarriers Encapsulated with IL‐27 High Expressive MSC Extracellular Vesicles for Inflammatory Bowel Disease Treatment
Authors: Nie Min , Huang Danqing , Chen Guopu , Zhao Yuanjin , Sun Lingyun 
Published: September 2023

AbstractMesenchymal stem cell (MSC) therapy is a promising candidate for inflammatory bowel disease (IBD) treatment, while overcoming the limitations of naive seeding cells function and realizing efficient intestinal targeting remains a challenge. Here, a bioadhesive microparticle carrying interleukin‐27 (IL‐27) MSC‐derived extracellular vesicles (MSCIL‐27 EVs) is developed to treat IBD. The MSCIL‐27 EVs prepared through lentivirus‐mediated gene transfection technology show ideal anti‐inflammatory and damage repair function. By encapsulating MSCIL‐27 EVs into dopamine methacrylamide‐modified hydrogel, a bioadhesive EVs microcarrier via microfluidic technology is fabricated. The resultant microcarriers exhibit ideal MSCIL‐27 EVs sustained release effect and effective wet adhesion property. Furthermore, the therapeutic potential of MSCIL‐27 EVs‐loaded microcarriers in treating IBD is demonstrated. Through giving IBD rats a rectal administration, it is found that the microcarriers can firmly anchor to the surface of colon, reduce the inflammatory response, and repair the damaged barrier. Therefore, the bioadhesive MSCIL‐27 EVs‐loaded microcarriers provide a promising strategy for the biomedical application of MSC‐derived EVs, and broaden the clinical potential of MSC therapy.
The Critical Role of The Piezo1/β‐catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity‐Induced Bone Loss
Authors: Hu Yuxiang , Tian Hongtao , Chen Wei , Liu Yunlu , Cao Yulin , Pei Hongxin , Ming Chaochang , Shan Cunqing , Chen Xihui , Dai Zhipeng , Yang Shuhua , Shao Zengwu , Lan Shenghui , Liu Yong , Tong Wei 
Published: September 2023

AbstractDisuse osteoporosis is characterized by decreased bone mass caused by abnormal mechanical stimulation of bone. Piezo1 is a major mechanosensitive ion channel in bone homeostasis. However, whether intervening in the action of Piezo1 can rescue disuse osteoporosis remains unresolved. In this study, a commonly‐used hindlimb‐unloading model is employed to simulate microgravity. By single‐cell RNA sequencing, bone marrow‐derived mesenchymal stem cells (BMSCs) are the most downregulated cell cluster, and coincidentally, Piezo1 expression is mostly enriched in those cells, and is substantially downregulated by unloading. Importantly, activation of Piezo1 by systemically‐introducing yoda1 mimics the effects of mechanical stimulation and thus ameliorates bone loss under simulated microgravity. Mechanistically, Piezo1 activation promotes the proliferation and osteogenic differentiation of Gli1+ BMSCs by activating the β‐catenin and its target gene activating transcription factor 4 (ATF4). Inhibiting β‐catenin expression substantially attenuates the effect of yoda1 on bone loss, possibly due to inhibited proliferation and osteogenic differentiation capability of Gli1+ BMSCs mediated by ATF4. Lastly, Piezo1 activation also slightly alleviates the osteoporosis of OVX and aged mice. In conclusion, impaired function of Piezo1 in BMSCs leads to insufficient bone formation especially caused by abnormal mechanical stimuli, and is thus a potential therapeutic target for osteoporosis.
3D Chiral Self‐Assembling Matrixes for Regulating Polarization of Macrophages and Enhance Repair of Myocardial Infarction
Authors: Yang Lei , Yang Li , Lu Kongli , Su Nan , Li Xueqin , Guo Shuoxiang , Xue Song , Lian Feng , Feng Chuanliang 
Published: September 2023

AbstractThe regulation of inflammatory response at the site of injury and macrophage immunotherapy is critical for tissue repair. Chiral self‐assemblies are one of the most ubiquitous life cues, which is closely related to biological functions, life processes, and even the pathogenesis of diseases. However, the role of supramolecular chiral self‐assemblies in the regulation of immune functions in the internal environment of tissues has not been fully explored yet. Herein, 3D supramolecular chiral self‐assembling matrixes are prepared to regulate the polarization of macrophages and further enhance the repair of myocardial infarction (MI). Experiments studies show that M‐type (left‐handed) self‐assembling matrixes significantly inhibit inflammation and promote damaged myocardium repair by upregulating M2 macrophage polarization and downstream immune signaling compared with P‐type (right‐handed), and R‐type (non‐chirality) self‐assembling matrixes. Classical molecular dynamics (MD) simulation demonstrates that M‐type self‐assembling matrixes display higher stereo‐affinity to cellular binding, which enhances the clustering of mechanosensitive integrin β1 (Itgβ1) and activates focal adhesion kinase (FAK) and Rho‐associated protein kinase (ROCK), as well as downstream PI3K/Akt1/mTOR signaling axes to promote M2 polarization. This study of designing a 3D chiral self‐assembling matrixes microenvironment suitable for regulating the polarization of macrophages will provide devise basis for immunotherapy with biomimetic materials.
Anomalous Elastic Evolution Induced by Copper Hopping in van der Waals Ferroelectric CuInP2S6
Authors: Shao Minghao , Yang Yuzhe , He Ri , Zhao Ruiting , Renshaw Wang X. , Zhong Zhicheng , Liu Houfang , Yang Yi , Ren Tian‐Ling 
Published: September 2023

AbstractVan der Waals (vdW) ferroelectric CuInP2S6 (CIPS) has great potential in post‐Moore's law electronics owing to their advantages of weak interlayer interaction, stable surface with free dangling bonds, and robust switchable spontaneous polarization. The flexoelectric effect is demonstrated as an alternative switching method for the design of ferroelectric domains in layered vdW CIPS. However, the investigation of the correlation between the polarization and elastic properties remains indistinct. Here, an elastic evolution is explored experimentally and theoretically in layered vdW CIPS with temperature, of which Young's modulus (EYoung) is determined by analyzing the force‐indentation responses of vdW ferroelectric CIPS. Interestingly, an anomalous leap in EYoung of CIPS from 35 to 65 GPa occurs when the temperature rises across the Curie temperature TC of ≈315 K. Deep potential molecular dynamic (DPMD) simulations identify that this abnormal behavior can be attributed to temperature‐dependent change of copper distribution and the local copper dynamic hopping, intertwined with the order‐disorder ferroelectric phase transition, which is quite different from the typical decrease of EYoung in the lattice constant due to temperature increasing. The exploration provides an important reference for the analysis of coupled mechanical properties and ferroelectricity in CIPS and its applications in flexible electronics.
Parental age effects and Rett syndrome
Authors: Fang Xiaolan , Baggett Lauren M. , Caylor Raymond C. , Percy Alan K. , Neul Jeffrey L. , Lane Jane B. , Glaze Daniel G. , Benke Tim A. , Marsh Eric D. , Motil Kathleen J. , Barrish Judy O. , Annese Fran E. , Skinner Steven A. 
Published: September 2023

AbstractRett syndrome (RTT) is a progressive neurodevelopmental disorder, and pathogenic Methyl‐CpG‐binding Protein 2 (MECP2) variants are identified in >95% of individuals with typical RTT. Most of RTT‐causing variants in MECP2 are de novo and usually on the paternally inherited X chromosome. While paternal age has been reported to be associated with increased risk of genetic disorders, it is unknown whether parental age contributes to the risk of the development of RTT. Clinical data including parental age, RTT diagnostic status, and clinical severity are collected from 1226 participants with RTT and confirmed MECP2 variants. Statistical analyses are performed using Student t‐test, single factor analysis of variance (ANOVA), and multi‐factor regression. No significant difference is observed in parental ages of RTT probands compared to that of the general population. A small increase in parental ages is observed in participants with missense variants compared to those with nonsense variants. When we evaluate the association between clinical severity and parental ages by multiple regression analysis, there is no clear association between clinical severity and parental ages. Advanced parental ages do not appear to be a risk factor for RTT, and do not contribute to the clinical severity in individuals with RTT.
Neighboring Molecular Engineering in Diels–Alder Chemistry Enabling Easily Recyclable Carbon Fiber Reinforced Composites
Authors: Gu Song , Xiao Yan‐Fang , Tan Shi‐Huan , Liu Bo‐Wen , Guo De‐Ming , Wang Yu‐Zhong , Chen Li 
Published: September 2023

AbstractAlthough a variety of dynamic covalent bonds have been successfully used in the development of diverse sustainable thermosetting polymers and their composites, solving the trade‐off between recovery efficiency and comprehensive properties is still a major challenge. Herein, a “one‐stone‐two‐birds” strategy of lower rotational energy barrier (Er) phosphate‐derived Diels–Alder (DA) cycloadditions was proposed for easily recyclable carbon fiber (CF)‐reinforced epoxy resins (EPs) composites. In such a strategy, the phosphate spacer with lower Er accelerated the segmental mobility and dynamic DA exchange reaction for network rearrangement to achieve high‐efficiency repairing, reprocessing of the EPs matrix and its composites and rapid nondestructive recycling of CF; meanwhile, incorporating phosphorus‐based units especially reduced their fire hazards. The resulting materials simultaneously showed excellent thermal/mechanical properties, superb fire safety and facile recyclability, realizing the concept of recycling for high‐performance thermosetting polymers and composites. This strategy is of great significance for understanding and enriching the molecular connotation of DA chemistry, making it potentially applicable to the design and development of a wide range of dynamic covalent adaptable materials toward practical cutting‐edge‐tech applications.
Stabilization and Binding of [V4O12]4− and Unprecedented [V20O54(NO3)]n− to Lysozyme upon Loss of Ligands and Oxidation of the Potential Drug VIVO(acetylacetonato)2
Authors: Ferraro Giarita , Tito Gabriella , Sciortino Giuseppe , Garribba Eugenio , Merlino Antonello 
Published: September 2023

AbstractHigh‐resolution crystal structures of lysozyme in the presence of the potential drug VIVO(acetylacetonato)2 under two different experimental conditions have been solved. The crystallographic study reveals the loss of the ligands, the oxidation of VIV to VV and the subsequent formation of adducts of the protein with two different polyoxidovanadates: [V4O12]4−, which interacts with lysozyme non‐covalently, and the unprecedented [V20O54(NO3)]n−, which is covalenty bound to the side chain of an aspartate residue of symmetry related molecules.
Divergent Synthesis of Chelating Aziridines and Cyclic(Alkyl)(Amino)Carbenes (CAACs) from Pyridyl‐Tethered Robust Azomethine Ylides
Authors: Baguli Sudip , Sarkar Subham , Nath Soumajit , Mallick Dibyendu , Mukherjee Debabrata 
Published: September 2023

AbstractAzomethine ylides are typically in situ generated synthons for making N‐heterocycles through cycloaddition reactions. But an offbeat aspect about them is the isomeric nature of aldiminium‐based azomethine ylides and (alkyl/aryl)(amino)carbenes, interconvertible by a formal 1,3‐H+ transfer. Herein, two thermally robust azomethine ylides with a N‐appended picolyl sidearm are isolated, which cyclize to pyaziridines at 80 °C but unprecedentedly result N−picoCAAC‐CuCl (CAAC=cyclic(alkyl)(amino)carbene) complexes when heated with CuCl at merely 60 °C. The pendant Npy, as revealed by computational analysis, plays a crucial role in this unusual 1,3‐H+ shift using a deprotonation‐protonation sequence, as well as in placing the CuCl at the carbenic site in tandem. The softer nature of Cu(I) is also critical. Chelating CAACs are rare and one with a N‐tethered additional donor is priorly unknown. Both N‐picoCAAC and pyaziridine are bidentate chelators giving highly active cationic Rh(I) catalysts for hydrosilylating unactivated olefins by Et3SiH. Notably, the pyaziridine‐Rh(I) is superior than the N‐picoCAAC‐Rh(I) catalyst.
An NIR Fluorescence Turn‐on and MRl Bimodal Probe for Concurrent Real‐time in vivo Sensing and Labeling of β‐Galactosidase
Authors: Yu Qiao , Zhang Lei , Jiang Mou , Xiao Long , Xiang Yunhui , Wang Ruifang , Liu Zhaoqing , Zhou Rui , Yang Minghui , Li Conggang , Liu Maili , Zhou Xin , Chen Shizhen 
Published: September 2023

AbstractTo realize sensing and labeling biomarkers is quite challenging in terms of designing multimodal imaging probes. In this study, we developed a novel β‐galactosidase (β‐gal) activated bimodal imaging probe that combines near‐infrared (NIR) fluorescence and magnetic resonance imaging (MRI) to enable real‐time visualization of activity in living organisms. Upon β‐gal activation, Gal‐Cy‐Gd‐1 exhibits a remarkable 42‐fold increase in NIR fluorescence intensity at 717 nm, allowing covalent labeling of adjacent target enzymes or proteins and avoiding molecular escape to promote probe accumulation at the tumor site. This fluorescence reaction enhances the longitudinal relaxivity by approximately 1.9 times, facilitating high‐resolution MRI. The unique features of Gal‐Cy‐Gd‐1 enable real‐time and precise visualization of β‐gal activity in live tumor cells and mice. The probe's utilization aids in identifying in situ ovarian tumors, offering valuable assistance in the precise removal of tumor tissue during surgical procedures in mice. The fusion of NIR fluorescence and MRI activation through self‐immobilizing target enzymes or proteins provides a robust approach for visualizing β‐gal activity. Moreover, this approach sets the groundwork for developing other activatable bimodal probes, allowing real‐time in vivo imaging of enzyme activity and localization.
Über die Kette (Pt), H2(1 atm)/HBr, Hg2Br2(s)/Hg in organischen Lösungsmitteln und ihren Gemischen mit Wasser Ü. (Pt), H2(1 atm)/HBr(m), Methanol (x), H2O(100‐x), Hg2Br2(s)/Hg
Authors: Schwabe K. , Ferse E. , Rudolph W. 
Published: September 2023

AbstractAus Ketten ohne Überführung (I und Ü) werden mittlere Aktivitätskoeffizienten von HBr in Wasser und Wasser‐Methanol‐Gemischen bestimmt. Die aus den Temperaturkoeffizienten zugänglichen thermodynamischen Größen wie Entropie, Enthalpie und freie Enthalpie werden angegeben. Für die Wasser‐Methanol‐Gemische als neues Lösungsmittel werden Standard‐EMK‐Werte sowie Medieneffekte berechnet.
The mechanism of the thermal decomposition of ethyl nitrite
Authors: Marta F. , Seres L. 
Published: September 2023

AbstractThe thermal decomposition of ethyl nitrite has been studied in the temperature range 200 – 230 °C and at pressures ranging from 20 – 200 Torr. The reaction was followed by pressure change and gas chromatographic measurements. The products of the decomposition were found to be nitric oxide, nitrous oxide, acetaldehyde, ethanol, acetal and water. The effect of the products on the rate of the decomposition and on the product distribution has also been investigated. The acetaldehyde increased the rate of the decomposition and the yield of acetal and ethanol, while it decreased the yield of nitrous oxide. The nitric oxide reduced the rate and the yield of acetal and increased the yield of acetaldehyde and that of nitrous oxide. The mechanism suggested is able to account for the experimental results.
Spinal accessory nerve anatomy in the posterior cervical triangle: A systematic review with meta‐analysis
Authors: Koliarakis Ioannis , Manatakis Dimitrios K. , Tsitsipanis Christos , Drakonaki Elena , Tsamandouras Ioannis , Tsiaoussis John 
Published: September 2023

AbstractThis study aimed to investigate the anatomy of the spinal accessory nerve (SAN) in the posterior cervical triangle, especially in relation to adjacent anatomical landmarks, along with a systematic review of the current literature with a meta‐analysis of the data. Overall, 22 cadaveric and three prospective intraoperative studies, with a total of 1346 heminecks, were included in the analysis. The major landmarks relevant to the entry of the SAN at the posterior border of the SCM muscle (PBSCM) were found to be the mastoid apex, the great auricular point (GAP), the nerve point (NP), and the point where the PBSCM meets the upper border of the clavicle. The SAN was reported to enter the posterior cervical triangle above GAP in 100% of cases and above NP in most cases (97.5%). The mean length of the SAN along its course from the entry point to its exit point from the posterior triangle of the neck was 4.07 ± 1.13 cm. The SAN mainly gave off 1 or 2 branches (32.5% and 31%, respectively) and received either no branches or one branch in most cases (58% and 23%, respectively) from the cervical plexus during its course in the posterior cervical triangle. The major landmarks relevant to the entry of the SAN at the anterior border of the TPZ muscle (ABTPZ) were found to be the point where the ABTPZ meets the upper border of the clavicle and the midpoint of the clavicle, along with the mastoid apex, the acromion, and the transverse distance of the SAN exit point to the PBSCM. The results of the present meta‐analysis will be helpful to surgeons operating in the posterior cervical triangle, aiding the avoidance of the iatrogenic injury of the SAN.
Deficiency of SR‐B1 reduced the tumor load of colitis‐induced or APCmin/+‐induced colorectal cancer
Authors: Chen Qijun , Wang Lixue , Song Hui , Xing Wen , Shi Junfeng , Li Yudi , Wang Ziqian , Chen Jinlong , Xie Nan , Zhao Wenhua 
Published: September 2023

AbstractBackgroundColorectal cancer (CRC) is one of the most common tumors in the world. Cholesterol plays an important role in the pathogenesis of tumors. One of the cholesterol transporters, scavenger receptor class B type 1 (SR‐B1), a multi‐ligand membrane receptor protein, is expressed in the intestines which also highly expressed in various tumors. But the potential mechanism of SR‐B1 in CRC development has not been reported.AimsThis study aimed to clarify the importance of SR‐B1 in the development and prognosis of CRC as much as possible to provide a possible strategy in CRC treatment.Materials & MethodsIn this study, we used SR‐B1 gene knockdown mice to study the effect of SR‐B1 on colitis‐induced or APCmin/+‐induced CRC. The expression of related molecules were detected through the immunohistochemistry and hematoxylin–eosin staining, western blot analysis, and Flow cytometry. The gene expression and microbiota in microenvironment of CRC mice were analyzed through eukaryotic mRNA sequencing and 16S rRNA high‐throughput sequencing.ResultsThe results showed that SR‐B1 knockdown reduced the tumor load of colitis‐induced or APCmin/+‐induced CRC. SR‐B1 knockdown improved the immune microenvironment by affecting the level of tumor‐associated macrophage (TAM), mononuclear myeloid‐derived suppressor cells (M‐MDSCs), granulocytic myeloid‐derived suppressor cells (G‐MDSCs), programmed cell death‐ligand 1 (PD‐L1), and human leukocyte antigen class I‐B (HLA‐B), and also reduced the level of low‐density lipoprotein receptor (LDL‐R), and increased the level of ATP binding cassette transporter A1 (ABCA1) to regulate the cholesterol metabolism, and regulated the expression of related genes and intestinal microbiota. SR‐B1 knockdown can also trigger the anti‐CRC effect of anti‐PD 1 in colitis‐induced CRC.DiscussionSR‐B1 deficiency significantly improved the immunity in tumor microenvironment of colitis‐induced or APCmin/+‐induced CRC. In addition, the microbiota changes caused by SR‐B1 deficiency favor improving the immune response to chemotherapeutic drugs and anti‐PD1 therapy. The mechanism of action of SR‐B1 deficiency on the development of CRC still needs further in‐depth research.ConclusionThis study provides a new treatment strategy for treating CRC by affecting the expression of SR‐B1 in intestine.
Estimating the overall survival benefit of adjuvant chemo‐endocrine therapy in women over age 50 with pT1‐2N0 early stage breast cancer and 21‐gene recurrence score ≥26: A National Cancer Database analysis
Authors: Stabellini Nickolas , Cao Lifen , Towe Christopher W. , Amin Amanda L. , Montero Alberto J. 
Published: September 2023

AbstractBackgroundValidation studies of the 21‐gene recurrence score (RS) previously demonstrated that adjuvant chemotherapy plus endocrine therapy (CET) was associated with a significant survival benefit in women with node negative breast cancer (BC) and RS >31. However, the TAILORx trial, did not quantify the benefit of adjuvant CET in older women with node negative hormone receptor positive (HR+) BC with RS ≥26. We hypothesized that CET would be associated with improved overall survival (OS) compared to endocrine therapy (ET) in women >50 with HR+/HER2‐node negative BC and RS ≥26.MethodsThe National Cancer Database (NCDB) was queried to identify women >50 with RS ≥26 ER+/HER2‐BC pT1‐2N0M0. Chi‐square and logistic regression analysis determined the difference between ET and CET. OS was analyzed using a multivariable Cox model.ResultsWe included 16,745 women—4740 (28.3%) received ET and 12,005 (71.7%) received CET. Women who received CET had: moderately (OR = 1.853, p < 0.001) or poorly/undifferentiated tumors (OR = 3.875, p < 0.001), pT2 (OR = 1.356, p < 0.001), or lymph‐vascular invasion (OR = 1.206, p = 0.001). After accounting for demographic and oncologic factors, 5‐year OS rates were significantly superior in women receiving CET vs. ET alone (95.4% vs. 92.0%, Hazard Ratio = 0.680, p < 0.001).ConclusionsWe observed that CET was associated with a clinically and statistically significant higher OS compared to ET alone in women >50 years of age with RS ≥26 pT1 and pT2 N0M0 HR+/HER2‐breast cancer, and which suggests that cytotoxic chemotherapy has an impact on reducing mortality that is independent of induction of premature ovarian failure.
Body mass index and incident cardiometabolic conditions in relation to obesity‐related cancer risk: A population‐based cohort study in Catalonia, Spain
Authors: Recalde Martina , Pistillo Andrea , Viallon Vivian , Fontvieille Emma , Duarte‐Salles Talita , Freisling Heinz 
Published: September 2023

AbstractBackgroundWe investigated the association between body mass index (BMI) and obesity‐related cancer risk among individuals with/without incident hypertension (HTN), type 2 diabetes mellitus (T2DM), and cardiovascular disease (CVD) and the joint associations of overweight/obesity (BMI ≥25 kg/m2) and each cardiometabolic condition with obesity‐related cancer riskMethodsWe conducted a population‐based cohort (n = 1,774,904 individuals aged ≥40 years and free of cancer and cardiometabolic conditions at baseline) study between 2010 and 2018 with electronic health records from Spain. Our main outcome measures were hazard ratios (HRs) for incident obesity‐related cancers and relative excess risk due to interaction (RERI).ResultsA total of 38,082 individuals developed obesity‐related cancers after a median of 8 years of follow‐up. The positive association between BMI and obesity‐related cancer risk was similar among individuals free of cardiometabolic conditions (hazard ratio, HR per 5 kg/m2: 1.08, 95% confidence interval, CI: 1.06–1.10) and with incident HTN (1.05, 1.01–1.08). The association among those with incident T2DM was null (0.98, 0.93–1.03). There was a positive additive interaction between overweight/obesity and CVD (relative excess risk due to interaction [RERI]: 0.19 [0.09, 0.30]), meaning that the combined association was 0.19 more than the sum of the individual associations. In contrast, a RERI of −0.24 (−0.28, −0.20) was observed for the combined association between overweight/obesity and T2DM.ConclusionsPublic health strategies to reduce overweight can help prevent cancer cases among the general population and individuals with incident HTN/CVD. Further, weight‐loss interventions seem to lead to a greater cancer risk reduction among individuals with CVD.