bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway
Authors: Wu Yi-Bo , Li Shen-Yi , Liu Jin-Yan , Xue Jia-Jia , Xu Jin-Fu , Chen Ting , Cao Tian-Yue , Zhou Hui , Wu Tian-Tian , Dong Chun-Lin , Qian Wei-Feng , Qiao Long-Wei , Hou Shun-Yu , Wang Ting , Shen Cong 
Published: October 2023

AbstractAs a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, NRSN2-AS1 stabilizes protein tyrosine kinase 2 (PTK2) to activate the β-catenin pathway via repressing MG-53-mediated ubiquitinated degradation of PTK2, thereby facilitating ovarian cancer progression. Rescue experiments verify the function of the NRSN2-AS1/PTK2/β-catenin axis and the effects of MG53 on this axis in ovarian cancer cells. In conclusion, this study demonstrates the key role of the NRSN2-AS1/PTK2/β-catenin axis for the first time and explores its potential clinical applications in ovarian cancer.
Exogenous H2S initiating Nrf2/GPx4/GSH pathway through promoting Syvn1-Keap1 interaction in diabetic hearts
Authors: Wang Mengyi , Tang Jingyuan , Zhang Shiwu , Pang Kemiao , Zhao Yajun , Liu Ning , Huang Jiayi , Kang Jiaxin , Dong Shiyun , Li Hongxia , Tian Zhen , Duan Binhong , Lu Fanghao , Zhang Weihua 
Published: October 2023

AbstractExcessive ROS accumulation contributes to cardiac injury in type 2 diabetes mellitus. Hydrogen sulfide (H2S) is a vital endogenous gasotransmitter to alleviate cardiac damage in diabetic cardiomyopathy (DCM). However, the underlying mechanisms remain unclear. In this study, we investigated the effects of NaHS administration in db/db mice via intraperitoneal injection for 20 weeks and the treatment of high glucose (HG), palmitate (PA) and NaHS in HL-1 cardiomyocytes for 48 h, respectively. H2S levels were decreased in hearts of db/db mice and HL-1 cardiomyocytes exposed to HG and PA, which were restored by NaHS. Exogenous H2S activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/glutathione peroxidase 4 (GPx4)/glutathione (GSH) pathway, suppressed ferroptosis and mitigated mitochondrial apoptosis in db/db mice. However, these effects were abrogated after Nrf2 knockdown. NaHS treatment elevated the ubiquitination level of Kelch-like ECH-associated protein (Keap1) by preserving its E3 ligase synoviolin (Syvn1), resulting in Nrf2 nuclear translocation. H2S facilitated the sulfhydration of Syvn1-cys115 site, a post-translational modification. Transfecting Syvn1 C115A in cardiomyocytes exposed to HG and PA partially attenuated the effects of NaHS on Nrf2 and cell death. Our findings suggest that exogenous H2S regulates Nrf2/GPx4/GSH pathway by promoting the Syvn1-Keap1 interaction to reduce ferroptosis and mitochondrial apoptosis in DCM.
Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes
Authors: Dong Jie , Jin Liping , Bao Shihua , Chen Biaobang , Zeng Yang , Luo Yuxi , Du Xingzhu , Sang Qing , Wu Tianyu , Wang Lei 
Published: October 2023

AbstractAneuploidy seriously compromises female fertility and increases incidence of birth defects. Rates of aneuploidy in human eggs from even young women are significantly higher than those in other mammals. However, intrinsic genetic factors underlying this high incidence of aneuploidy in human eggs remain largely unknown. Here, we found that ectopic expression of humanTUBB8in mouse oocytes increases rates of aneuploidy by causing kinetochore–microtubule (K–MT) attachment defects. Stretched bivalents in mouse oocytes expressingTUBB8are under less tension, resulting in continuous phosphorylation status of HEC1 by AURKB/C at late metaphase I that impairs the established correct K–MT attachments. This reduced tension in stretched bivalents likely correlates with decreased recruitment of KIF11 on meiotic spindles. We also found that ectopic expression ofTUBB8without its C-terminal tail decreases aneuploidy rates by reducing erroneous K–MT attachments. Importantly, variants in the C-terminal tail ofTUBB8were identified in patients with recurrent miscarriages. Ectopic expression of an identifiedTUBB8variant in mouse oocytes also compromises K–MT attachments and increases aneuploidy rates. In conclusion, our study provides novel understanding for physiological mechanisms of aneuploidy in human eggs as well as for pathophysiological mechanisms involved in recurrent miscarriages.
Integrated radiogenomics models predict response to neoadjuvant chemotherapy in high grade serous ovarian cancer
Authors: Crispin-Ortuzar Mireia , Woitek Ramona , Reinius Marika A. V. , Moore Elizabeth , Beer Lucian , Bura Vlad , Rundo Leonardo , McCague Cathal , Ursprung Stephan , Escudero Sanchez Lorena , Martin-Gonzalez Paula , Mouliere Florent , Chandrananda Dineika , Morris James , Goranova Teodora , Piskorz Anna M. , Singh Naveena , Sahdev Anju , Pintican Roxana , Zerunian Marta , Rosenfeld Nitzan , Addley Helen , Jimenez-Linan Mercedes , Markowetz Florian , Sala Evis , Brenton James D. 
Published: October 2023

AbstractHigh grade serous ovarian carcinoma (HGSOC) is a highly heterogeneous disease that typically presents at an advanced, metastatic state. The multi-scale complexity of HGSOC is a major obstacle to predicting response to neoadjuvant chemotherapy (NACT) and understanding critical determinants of response. Here we present a framework to predict the response of HGSOC patients to NACT integrating baseline clinical, blood-based, and radiomic biomarkers extracted from all primary and metastatic lesions. We use an ensemble machine learning model trained to predict the change in total disease volume using data obtained at diagnosis (n = 72). The model is validated in an internal hold-out cohort (n = 20) and an independent external patient cohort (n = 42). In the external cohort the integrated radiomics model reduces the prediction error by 8% with respect to the clinical model, achieving an AUC of 0.78 for RECIST 1.1 classification compared to 0.47 for the clinical model. Our results emphasize the value of including radiomics data in integrative models of treatment response and provide methods for developing new biomarker-based clinical trials of NACT in HGSOC.
Recognition and coacervation of G-quadruplexes by a multifunctional disordered region in RECQ4 helicase
Authors: Papageorgiou Anna C. , Pospisilova Michaela , Cibulka Jakub , Ashraf Raghib , Waudby Christopher A. , Kadeřávek Pavel , Maroz Volha , Kubicek Karel , Prokop Zbynek , Krejci Lumir , Tripsianes Konstantinos 
Published: October 2023

AbstractBiomolecular polyelectrolyte complexes can be formed between oppositely charged intrinsically disordered regions (IDRs) of proteins or between IDRs and nucleic acids. Highly charged IDRs are abundant in the nucleus, yet few have been functionally characterized. Here, we show that a positively charged IDR within the human ATP-dependent DNA helicase Q4 (RECQ4) forms coacervates with G-quadruplexes (G4s). We describe a three-step model of charge-driven coacervation by integrating equilibrium and kinetic binding data in a global numerical model. The oppositely charged IDR and G4 molecules form a complex in the solution that follows a rapid nucleation-growth mechanism leading to a dynamic equilibrium between dilute and condensed phases. We also discover a physical interaction with Replication Protein A (RPA) and demonstrate that the IDR can switch between the two extremes of the structural continuum of complexes. The structural, kinetic, and thermodynamic profile of its interactions revealed a dynamic disordered complex with nucleic acids and a static ordered complex with RPA protein. The two mutually exclusive binding modes suggest a regulatory role for the IDR in RECQ4 function by enabling molecular handoffs. Our study extends the functional repertoire of IDRs and demonstrates a role of polyelectrolyte complexes involved in G4 binding.
Cu-catalyzed asymmetric regiodivergent electrosynthesis and its application in the enantioselective total synthesis of (-)-fumimycin
Authors: Xie Tian , Huang Jianming , Li Juan , Peng Lingzi , Song Jin , Guo Chang 
Published: October 2023

AbstractQuaternary amino acids are one of the essential building blocks and precursors of medicinally important compounds. Various synthetic strategies towards their synthesis have been reported. On the other hand, developing core-structure-oriented cross-dehydrogenative coupling (CDC) reactions, is a largely unsolved problem. Herein, we describe a copper-catalyzed regiodivergent electrochemical CDC reaction of Schiff bases and commercially available hydroquinones to obtain three classes of chiral quaternary amino acid derivatives for the efficient assembly of complex scaffolds with excellent stereocontrol. The electrochemical anodic oxidation process with slow releasing of quinones serves as an internal syringe pump and provides high levels of reaction efficiency and enantiomeric control. The utility of this strategy is highlighted through the synthetic utility in the asymmetric total synthesis of (-)-fumimycin.
PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs
Authors: Contreras Xavier , Depierre David , Akkawi Charbel , Srbic Marina , Helsmoortel Marion , Nogaret Maguelone , LeHars Matthieu , Salifou Kader , Heurteau Alexandre , Cuvier Olivier , Kiernan Rosemary 
Published: October 2023

AbstractPervasive transcription of the human genome generates an abundance of RNAs that must be processed and degraded. The nuclear RNA exosome is the main RNA degradation machinery in the nucleus. However, nuclear exosome must be recruited to its substrates by targeting complexes, such as NEXT or PAXT. By proteomic analysis, we identify additional subunits of PAXT, including many orthologs of MTREC found in S. pombe. In particular, we show that polyA polymerase gamma (PAPγ) associates with PAXT. Genome-wide mapping of the binding sites of ZFC3H1, RBM27 and PAPγ shows that PAXT is recruited to the TSS of hundreds of genes. Loss of ZFC3H1 abolishes recruitment of PAXT subunits including PAPγ to TSSs and concomitantly increases the abundance of PROMPTs at the same sites. Moreover, PAPγ, as well as MTR4 and ZFC3H1, is implicated in the polyadenylation of PROMPTs. Our results thus provide key insights into the direct targeting of PROMPT ncRNAs by PAXT at their genomic sites.
New diffusive interface model for pitting corrosion
Authors: Li Bohan , Xing Hui , Jing Hanxu 
Published: October 2023

AbstractIn this paper, a diffusive interface model for pitting corrosion is proposed, which can be used to simulate diffusion- and activation-controlled pitting corrosion with the pit morphologies and the distribution of metal cation concentration. The diffusive interface equation is derived from the general interface advection-like equation coupled with the description of the normal velocity of the moving pitting boundary from the sharp interface model. The corrosion rate is expressed in an explicit way through the diffusion field in the diffusion-controlled regime, and the activation-controlled corrosion is expressed by introducing the interface kinetic parameter, which greatly simplifies the model. The curvature effect can be subtracted back out directly by introducing a counter term. Predicted results from numerical simulations are compared with the analytical solution to determine numerical parameter in one-dimensional pencil electrode test. The effect of local curvature on simulated semicircle corrosion is discussed. Then, the application scenarios of the model including electropolishing, single and multiple corrosion, corrosion with composite materials are presented.
All-photonic one-way quantum repeaters with measurement-based error correction
Authors: Niu Daoheng , Zhang Yuxuan , Shabani Alireza , Shapourian Hassan 
Published: October 2023

AbstractQuantum repeater is the key technology enabler for long-distance quantum communication. To date, most of the existing quantum repeater protocols are designed based on specific quantum codes or graph states. In this paper, we propose a general framework for all-photonic one-way quantum repeaters based on the measurement-based error correction, which can be adapted to any Calderbank–Shor–Steane code including the recently discovered quantum low-density parity check (QLDPC) codes. We present a decoding scheme, where the error correction process is carried out at the destination based on the accumulated data from the measurements made across the network. This procedure not only outperforms the conventional protocols with independent repeaters but also simplifies the local quantum operations at repeaters. As an example, we numerically show that the [[48, 6, 8]] generalized bicycle code (as a small but efficient QLDPC code) has an equally good performance while reducing the resources by at least an order of magnitude.
Non-pharmaceutical interventions to combat COVID-19 in the Americas described through daily sub-national data
Authors: Touchton Michael , Knaul Felicia Marie , Arreola-Ornelas Héctor , Porteny Thalia , Carniado Óscar Méndez , Faganello Marco , Hummel Calla , Otero Silvia , Insua Jorge , Patino Fausto , Undurraga Eduardo , Pérez-Cruz Pedro , Sanchez-Talanquer Mariano , Velasco Guachalla V. Ximena , Nelson-Nuñez Jami , Boulding Carew , Calderon-Anyosa Renzo , Garcia Patricia J , Vargas Enciso Valentina 
Published: October 2023

AbstractThis dataset covers national and subnational non-pharmaceutical interventions (NPI) to combat the COVID-19 pandemic in the Americas. Prior to the development of a vaccine, NPI were governments’ primary tools to mitigate the spread of COVID-19. Variation in subnational responses to COVID-19 is high and is salient for health outcomes. This dataset captures governments’ dynamic, varied NPI to combat COVID-19 for 80% of Latin America’s population from each country’s first case through December 2021. These daily data encompass all national and subnational units in Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Mexico, and Peru. The dataset includes individual and aggregate indices of nine NPI: school closures, work suspensions, public event cancellations, public transport suspensions, information campaigns, local travel restrictions, international travel controls, stay-at-home orders, and restrictions on the size of gatherings. We also collected data on mask mandates as a separate indicator. Local country-teams drew from multiple data sources, resulting in high-quality, reliable data. The dataset thus allows for consistent, meaningful comparisons of NPI within and across countries during the pandemic.
Macroeconomic dynamics in a finite world based on thermodynamic potential
Authors: Herbert Éric , Giraud Gaël , Louis-Napoléon Aurélie , Goupil Christophe 
Published: October 2023

AbstractThis paper presents a conceptual model describing the medium and long term co-evolution of natural and socio-economic subsystems of Earth. An economy is viewed as an out-of-equilibrium dissipative structure that can only be maintained with a flow of energy and matter. The distinctive approach emphasized here consists in capturing the economic impact of natural ecosystems’ depletion by human activities via a pinch of thermodynamic potentials. This viewpoint allows: (i) the full-blown integration of a limited quantity of primary resources into a non-linear macrodynamics that is stock-flow consistent both in terms of matter-energy and economic transactions; (ii) the inclusion of natural and forced recycling; (iii) the inclusion of a friction term which reflects the impossibility to produce (and recycle)goods and services without exuding energy and matter wastes, and (iv) the computation of the anthropically produced entropy as a function of metabolizing intensity and frictions. Analysis and numerical computations confirm the role played by intensity and frictions as key factors for sustainability by contrast with real gdp growth—as well as the interplay between resource scarcity, income inequality, and inflation. A more egalitarian society with moderate inflation turns out to be more sustainable than an unequal society with low inflation. Our approach is flexible enough to allow for various economic models to be embedded into our thermodynamic framework. Finally, we propose the open source EcoDyco software as a first complete realization implementing economic dynamics in a multi-resource environment.
Landscape genetics identified conservation priority areas for blue sheep (Pseudois nayaur) in the Indian Trans-Himalayan Region
Authors: Dolker Stanzin , Jabin Gul , Singh Sujeet Kumar , Joshi Bheem Dutt , Singh Vinaya Kumar , Dalui Supriyo , Chandra Kailash , Sharma Lalit Kumar , Thakur Mukesh 
Published: October 2023

AbstractThe trans-Himalayan region of India, although have xeric features, still supports a unique assemblage of biodiversity, including some of the charismatic and endemic species. In the present study, we studied blue sheep (Pseudois nayaur) across the distribution range in the Western trans Himalayas of India and found about 18,775 km2 area suitable for blue sheep. The explicit Bayesian based spatial and non-spatial population structure analysis assigned blue sheep into two genetic populations, i.e., Ladakh and Lahaul-Spiti. We found relatively high genetic divergence in blue sheep which is also supported by the low current flow in Circuitscape model. With the multiple evidences, we explain landscape resistance facilitated by the landscape heterogeneity, and large patches of unsuitable habitats forced population divergence and poor functional connectivity. We found that blue sheep population has been demographically stable in the past, but showed a slight decline within the last few decades. This study is the first range-wide attempt to exhibit landscape features in shaping the spatial distribution, genetic structure and demography patterns of blue sheep in Western Himalayas, and will be of use in the conservation and management planning of blue sheep.
Combined effect of the pro-apoptotic rhTRAIL protein and HSV-1 virus in head and neck cancer cell lines
Authors: Bravo Perina Lucas , Faria Gomes Izabela Natalia , Alcantara Pelloso Ana Rúbia , Silva Viviane Aline Oliveira , Rebolho Batista Arantes Lidia Maria , Eliseo Melendez Matias 
Published: October 2023

AbstractKnowledge on the molecular and clinical characteristics of head and neck squamous cell carcinoma (HNSCC) is vast. However, an effective therapy that increases the life expectancy of these patients, with a 5-year overall survival of 50%, is still unknown. Here we evaluated the combined effect of the pro-apoptotic protein rhTRAIL with the replication-competent wild-type HSV-1 virus in head and neck cancer cell lines. We observed a difference in the modulation profile of proteins related to apoptotic pathways in the studied cell lines. The HCB289 exhibited caspase-9 activation in the presence of the HSV-1 virus, while the UD-SCC-2 exhibited caspase-8 activation in the presence of rhTRAIL. Both cell lines exhibited PARP activation by combining rhTRAIL and HSV-1 virus treatment. Flow cytometry analysis exhibited greater induction of late apoptosis for the HCB289 and UD-SCC-2 after the combination treatment of the HSV-1 and rhTRAIL. However, the UD-SCC-2 also presented induction of late apoptosis by the presence of rhTRAIL in monotherapy. These data suggest an enhancement of the effect of the combination treatment of the rhTRAIL and the HSV-1 on reducing viability and induction of cell death.
Deficit irrigation strategies (PRD, SDI) and titanium nanoparticles improve water use efficiency and flower quality in greenhouse-grown cut roses
Authors: Alavi Seyed Mohammad , Kamali Maryam , Selahvarzi Yahya , Ansari Sana 
Published: October 2023

AbstractThis study explored the use of deficit irrigation techniques for water management in the hydroponic greenhouse cultivation of cut roses. A factorial experiment was conducted using three irrigation treatments: full irrigation (FI), partial root drying (PRD), and sustained deficit irrigation (SDI), and three doses of titanium dioxide nanoparticle foliar application (0, 15, and 30 ppm) as stress alleviation. Results showed that drought stress increased biochemical parameters such as the plants' proline and total phenol content. Compared to SDI treatment, the PRD treatments have an increase in flower number by 40%. The PRD strategy has positive effects on drought tolerance by increasing osmotic and elastic adjustment. Therefore, higher relative water content and longer root length in PRD treatments were observed. Thus, Biomass water use efficiency significantly increased in PRD treatments compared to others. In the PRD treatment, yield WUE increases by 26% and 61% compared to FI and SDI, respectively. The results showed TiO2-NPs positively affected mitigating and even improving some traits in drought stress conditions. These results suggest the superiority of the PRD strategy, which improves growth characteristics and water use efficiency, leading to increased sustainability, reduced environmental impact of greenhouse toxic wastewater, and total profitability of the greenhouse.
Temperature-dependent complex dielectric permittivity: a simple measurement strategy for liquid-phase samples
Authors: Baker-Fales Montgomery , Gutiérrez-Cano José D. , Catalá-Civera José M. , Vlachos Dionisios G. 
Published: October 2023

AbstractMicrowaves (MWs) are an emerging technology for intensified and electrified chemical manufacturing. MW heating is intimately linked to a material’s dielectric permittivity. These properties are highly dependent on temperature and pressure, but such datasets are not readily available due to the limited accessibility of the current methodologies to process-oriented laboratories. We introduce a simple, benchtop approach for producing these datasets near the 2.45 GHz industrial, medical, and scientific (ISM) frequency for liquid samples. By building upon a previously-demonstrated bireentrant microwave measurement cavity, we introduce larger pressure- and temperature-capable vials to deduce temperature-dependent permittivity quickly and accurately for vapor pressures up to 7 bar. Our methodology is validated using literature data, demonstrating broad applicability for materials with dielectric constant ε' ranging from 1 to 100. We provide new permittivity data for water, organic solvents, and hydrochloric acid solutions. Finally, we provide simple fits to our data for easy use.
Gene profiles and mutations in the development of cataracts in the ICR rat model of hereditary cataracts
Authors: Takashima Masaru , Taniguchi Kei , Nagaya Masaya , Yamamura Shunki , Takamura Yoshihiro , Inatani Masaru , Oki Masaya 
Published: October 2023

AbstractCataracts are opacifications of the lens that cause loss of visual acuity and ultimately of eyesight. Age-related cataract develops in most elderly people, but the mechanisms of cataract onset are incompletely understood. The Ihara Cataract Rat (ICR) is an animal model of hereditary cataracts showing cortical opacity that commonly develops prematurely. We identified putative mechanisms of cataract onset in the ICR rat model by measuring gene expression changes before and after cortical cataract development and conducting point mutation analysis. Genes differentially expressed between 4-week-old animals without cortical cataracts and 8–10-week-old animals with cortical cataracts were selected from microarray analysis. Three connections were identified by STRING analysis: (i) Epithelial-Mesenchymal Transition (EMT), including Col1a2, and Pik3r1. (ii) Lens homeostasis, including Aqp5, and Cpm. (iii) Lipid metabolism, including Scd1, Srebf1, and Pnpla3. Subsequently, mutation points were selected by comparing ICR rats with 12 different rats that do not develop cataracts. The apolipoprotein Apoc3 was mutated in ICR rats. Analyses of gene expression changes and point and mutations suggested that abnormalities in EMT or lipid metabolism could contribute to cataract development in ICR rats.