bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential newborn care practices at health facilities and homes in Ethiopia: A cross-sectional study
Authors: Guadu Tadesse , Alemu Kassahun , Schellenberg Joanna , Berhanu Della , Lemma Seblewengel , Defar Atkure , Getachew Theodros , Taye Girum , Shiferaw Solomon , Abdissa Zewditu , Tariku Amare , Zelalem Meseret , Persson Lars Åke 
Published: December 2023

Abstract
Background
WHO recommends a set of essential newborn care practices that every newborn baby needs for health and survival regardless of birthplace. Previous Ethiopian studies of these actions had long recall periods, and most considered either facility or home deliveries. We aimed to assess selected practices, i.e., thermal care, cord care, initiation of breastfeeding, and first six-weeks immunizations among neonates born at health facilities and homes in Ethiopia.
Methods
We used data from the Performance Monitoring for Action Ethiopia 2019–2021 survey and analyzed 2,493 live births who completed interviews at six weeks postpartum in five regions, namely Tigray, Afar, Amhara, Oromia, Southern Nations, Nationalities and Peoples regions, and Addis Ababa. Practices were assessed using an interviewer-administered questionnaire. Essential newborn care indicators were analyzed among neonates born at health facilities and homes and expressed as proportions with a 95% confidence interval. Clustering was accounted for in all analyses.
Results
Practices were assessed among 1543 (62%) facility-delivered and 950 (38%) home-delivered neonates. Some of the appropriate actions had higher coverage among facility-delivered vs. home-delivered neonates: skin-to-skin care 76% vs. 8%, immediate drying and wrapping with a cloth 80% vs. 79%, delayed bathing until 24 hours after birth 72% vs. 39%, appropriate instrument to cut the cord 100% vs. 94%, use of new or boiled instrument 90% vs. 79%, nothing or chlorhexidine applied on cord 89% vs. 64%, first-hour initiation of breastfeeding 69% vs. 62%, and BCG and first polio immunization within the first six weeks after birth 54% vs. 19%. Thermal care, cord care and provision of vaccines at birth were relatively better in Addis Ababa, while first-hour breastfeeding initiation was higher in Oromia region.
Conclusion
Neonates born at health facilities had higher, although not optimal, coverage of thermal care, cord care, early initiation of breastfeeding, and the first immunizations. Strengthening the health extension program to promote facility delivery, further enhancing the coverage and quality of essential newborn care at health facilities, and increasing the coverage of first vaccinations and other essential services after birth is crucial for improving neonatal health and survival in Ethiopia.
The hallmark of the NhaA structural fold-crossing of two transmembranes- is critical for antiporter activity
Authors: Rimon Abraham , Amartely Hadar , Padan Etana 
Published: December 2023

Abstract
Cell pH and Na+ homeostasis requires Na+/H+ antiporters. The crystal structure of NhaA, the main Escherichia coli Na+/H+ antiporter, revealed a unique NhaA structural fold shared by prokaryotic and eukaryotic membrane proteins. Out of the 12 NhaA transmembrane segments (TMs), TMs III–V and X–XII are topologically inverted repeats with unwound TMs IV and XI forming the X shape characterizing the NhaA fold. We show that intramolecular cross-linking under oxidizing conditions of a NhaA mutant with two Cys replacements across the crossing (D133C-T340C) inhibits antiporter activity and impairs NhaA-dependent cell growth in high-salts. The affinity purified D133C-T340C protein binds Li+ (the Na+ surrogate substrate of NhaA) under reducing conditions. The cross-linking traps the antiporter in an outward-facing conformation, blocking the antiport cycle. As many secondary transporters are found to share the NhaA fold, including some involved in human diseases, our data have importance for both basic and clinical research.
Thai Grammatical Error Correction for Deaf Students
Authors: Traitruengsakul Supachan , Chuangsuwanich Ekapol 
Published: December 2023

Abstract
Deaf students encounter challenges in written communication due to errors such as insertion, deletion, disorder, misusage, and misspellings. Grammatical error correction (GEC) technology can help mitigate these issues. However, existing GEC models are primarily trained on online resources from second-language hearing learners. In contrast, sentences written by deaf students suffer from a variety of errors not typically found elsewhere.To address this issue, we create the Thai Deaf Corpus (TDC), focusing on identifying and analyzing errors among deaf students in grades 7-12 across four deaf schools. Additionally, we introduce a two-stage system for the Thai-GEC model, automatically detecting and correcting incorrect words in ungrammatical sentences written by deaf students.In our experiment, we compare the performance of the recurrent neural networks (RNN) detection model with and without feature embeddings from different sources—TDC and News corpus; moreover, we compare three correction models: WangchanBERTa and Seq2Seq models with and without pretraining. Our analysis of the TDC shows that deaf students use simpler words and shorter sentences compared to hearing peers, who often use complex vocabulary and lengthy sentences. The RNN detection model with feature embeddings learned from only TDC can outperform the others. The Seq2Seq correction model with pretraining outperforms others as it learns from formal and grammatical sentences in the News corpus. The dataset is made available at https://github.com/Supachan/ThaiDeafCorpus.git
First records of Secretargas transgariepinus (Argasidae) in Libya and Jordan: corrections of collection records and detection of microorganisms
Authors: Ševčík Martin , Špitalská Eva , Maliterná Michaela , Kabát Peter , Benda Petr 
Published: December 2023

Abstract

The primarily bat-associated argasid, Secretargas transgariepinus (White, 1846), is a member of the Afrotropical and southern Palaearctic fauna. Probably because of its secretive life style, little is known about this species and records of its collection are scant. Based on morphological revisions of the available specimens, we report new Middle Eastern records for this tick species, that had been misidentified as other bat-associated argasid taxa. These specimens are larvae from three localities, and represent the first records of S. transgariepinus from two countries: one larva from Sabratha (Libya) was collected from an unidentified bat (possibly Eptesicus isabellinus), seven larvae from Azraq-Shishan (Jordan), and 78 larvae from Shamwari (Jordan) were all collected from Otonycteris hemprichii. Twenty larvae from Shamwari were also tested for the presence of both, viral or bacterial microorganisms by PCR. Three ticks were found to be infected with the Murine gammaherpesvirus 68, one with Borrelia burgdorferi sensu lato, and four with a Rickettsia sp. closely related to Rickettsia slovaca. The findings represent a first evidence for the occurrence of these possible pathogens in S. transgariepinus.
Transient Heat Flux Assessment Using a Platinum Thin Film Sensor for Short-Duration Applications
Authors: Dongare Sumedh , Peetala Ravi K. , Gohil Trushar B. , Agrawal Nidhish , Jadhav Akash 
Published: December 2023

Abstract
The rapid fluctuations in heat transfer rates make it challenging to determine the surface temperature history and the estimation of accurate heat generation in research applications such as IC engines, gas turbines, and high-speed space vehicles. Therefore, thin-film gauges (TFGs) are generally used to measure the heat flux in such applications due to their high sensitivity and quick response time. The present study demonstrates that increasing the annealing heat treatment temperature, will enhance the adhesion of the thin film and the capabilities of these handmade thin-film gauges for transient measurements at low temperatures and for short periods. In the present work, TFG is fabricated in-house using platinum as a sensing element and Macor as an insulating substrate. The sensitivity (S) and temperature coefficient of resistance (TCR) are estimated using an oil batch calibration technique. At the same time, the performance of TFG is tested in a dynamic convective environment. The TFG is exposed to the convective environment using a designed calibration setup , and their transient heat fluxes are computed by conducting several trials. Additionally, the numerical solution has been accomplished using various experimental parameters. In comparison to the outcomes of the experimental method, it is observed that the average fluctuating temperature and mean surface heat flux have an inaccuracy of 0.33% and 4.17% respectively.
The Effect of Family-Oriented Organized Motivational Program on Working Memory in the Children with Autism Spectrum Disorder
Authors: Afshari Javad , Afshari Nahideh 
Published: December 2023

Abstract
Working memory plays an important role in learning. In this study, we are looking for motivational ways to stimulate memory. The participants (8 girls and 10 boys) were divided into experimental and control groups. They are selected from among 28 subjects after primary tests to be matched. The design of the study is semi-experimental including an independent variable, a pretest and a posttest. After considering the research hypotheses, using descriptive statistics and one-way Analysis of Covariance (ANCOVA), the results suggested a significant difference in posttest scores of experimental and control groups after independent variable being applied. The results show that a motivational program including math assignments and naming shapes assignments that it has coincided with children's motivations can has a positive effect on working memory in children with autism spectrum disorders. Due to increase in brain cognitive functions which control motivation and memory, memory experienced a high level of performance which can lead to more learning.
Time-variation study and OSMAC approach on fumonisin B1 and other mycotoxin production by Fusarium verticillioides.
Authors: Groppi Emie , Gadea Alice , Cristofoli Valérie , Vansteelandt Marieke , Haddad Mohamed 
Published: December 2023

Abstract

Fusarium verticillioides is a plant pathogenic fungus that can cause disease in a variety of crops, including corn, sorghum, and cotton. It is commonly found in soil, but can also be found in infected seeds and plant debris. This fungus produces several mycotoxins, including fumonisins, which can be harmful to both humans and animals if ingested in high concentrations. It can also produce other toxins such as "emerging" mycotoxins like beauvericin, fusaproliferin and others. In this study, we aimed to expand our understanding of mycotoxin production by F. verticillioides according to the substrate. To do so, we investigated the ecophysiology of F. verticillioides under different culture conditions (media, humidity, temperature) over time through UHPLC-HRMS/MS analysis and a dereplicative approach. Our results showed a modification of the fungal growth but also a variation of the nature and the relative concentrations of the mycotoxins produced along the time according to the modifications of culture conditions. This experiment showed that corn meal agar (CMA) medium (MEA) was favorable for fumonisin A1 (FA1), fumonisin B1 (FB1) and fumonisin B2 (FB2) production while malt extract agar medium was favorable for fumonisin A2 (FA2) production. This study allowed us to understand the development conditions of this fungus and the occurrence of its mycotoxins depending on its substrate.
Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries
Authors: Hu Qicai , Gui Yiqian , Cao Congcong , Xie Jun , Tang Huiru 
Published: December 2023

Abstract
Estrogen receptor α (ERα) is a crucial regulator of reproductive function, mediating the diverse effects of estrogen on the development and function of the ovary. However, the molecular mechanisms underlying ERα-regulated transcriptional dynamics in ovarian cells remain poorly understood. In the present study, we generated ERα knockout (αERKO) mice through CRISPR/Cas9 strategy. Then we employed single-cell RNA sequencing technology (scRNA-seq) to profile the transcriptomes of individual cells in wild type (WT) and αERKO mouse ovaries to uncover novel insights into ERα-regulated transcriptional change and ovarian cellular heterogeneity. Using several bioinformatics analyses, our study identified distinct cell populations within the ovarian tissue, including 5 distinct cell clusters associated with oocytes and 7 with granulosa cells. We further conducted differential gene expression analysis to explore the transcriptional profiles of these cell populations in response to ERα deletion. Our findings revealed that ERα deletion led to the dysregulation of genes involved in ovarian infertility, ovulation cycle, and steroidogenesis in oocytes and granulosa cells, indicating that ERα plays a central role in modulating the transcriptional landscape of ovarian cells. Consequently, we confirmed that Greb1, a prototypical ERα target gene, was induced by ERα binding to ERE upstream of the Greb1 promoter in granulosa cells. Additionally, we uncovered a complex network of signaling pathways that were significantly modulated by ERα, implicating their involvement in the regulation of ovarian function and ovulation process. In conclusion, our study offers an in-depth, single-cell resolution of ERα-regulated transcriptional dynamics in mouse ovaries, enhancing our understanding of ERα's role in female reproductive biology and providing potential targets for novel therapeutic interventions for ovarian disorders.
A first alert of Biomphalaria pfeifferi in the Lower Shire, Southern Malawi, a keystone intermediate snail host for intestinal schistosomiasis
Authors: Nkolokosa Clinton , Mbewe Rex , Chirombo James , Stanton Michelle C. , Jones Christopher M. , Makaula Peter , Namacha Gladys , Chiepa Blessings , Kalonde Patrick Ken , Baluwa Chifuniro , Zembere Kennedy , Kambewa Aisha , Kaonga Chikumbusko Chiziwa , Archer John , Juhász Alexandra , Cunningham Lucas J. , Tangena Julie-Anne Akiko , Stothard J. Russell 
Published: December 2023

Abstract

Repeated malacological surveys were conducted in Chikwawa and Nsanje Districts in the Lower Shire River, Southern Region of Malawi to alert to and to characterize populations of Biomphalaria pfeifferi. Sampling took place across a total of 45 sites, noting water conductivity, pH, temperature, total dissolved salts (TDS) and geographical elevation. Subsequently, the presence or absence of snails was predicted upon physiochemical and environmental conditions in Random Forest modelling. A concurrent molecular phylogenetic analysis of snails was conducted alongside molecular xenomonitoring for the presence of pre-patent infection with Schistosoma mansoni. Water conductivity, TDS and geographical elevation were most important in predicting abundance of snails with water temperature and pH of slightly less important roles. Our first alert with geographical modelling of populations of B. pfeifferi in the Lower Shire River is a critical step towards improving understanding of the transmission of intestinal schistosomiasis and a more solid foundation towards developing complementary strategies to mitigate it.
Exploring Gene Expression Dynamics for Sustainable Yield Enhancement in Chickpea
Authors: Mahto Rohit Kumar , B.S. Chandana , Singh Rajesh Kumar , Kumar Arun , Kumar Sudhir , Yadav Renu , Dey Debashish , Kumar Rajendra 
Published: December 2023

Abstract
Background
Chickpea, scientifically known as Cicer arietinum, is considered one of the essential crops due to its significant agricultural and economic importance. These leguminous plants are relatively large in genome size with 738 megabases. Chickpea roots play a crucial role in symbiotic relationships by forming nodules.
Results
20 genotypes were selected from an association panel meant for genome wide association studies on number of nodules and other nodulation traits. Before planting the seeds, the soil was prepared by mixing fertilizers and the specific microorganisms as biofertilizers viz; VAM and Rhizobium. The biofertilizers namely VAM, NPK, Rhizobium, and their 8 combinations in randomized block design with 3 replications were used as growing medium. The treatment mixture of NPK and Rhizobium showed significant performance in all the 20 genotypes by recognizing a genotype as the best one for the trait number of nodules. The morphological and molecular data observed on the subset comprising of 20 genotypes was subjected to descriptive statistics, correlation, path analysis, agglomerative hierarchical clustering, principal component analysis, gene expression analysis and identified the combination of Rhizobiumand VAM the best treatment along with novel donor as ICC- 9085 for the trait number of nodules and to achieve chickpea sustainable productivity.
Conclusions
The study shows the significant positive effects of VAM, NPK, Rhizobium, and their combinations playing an important role in improving the worldwide chickpea yield and production. The combination of Rhizobium and VAM treatments has shown great promise in stimulating nodulation in chickpea plants. These discoveries provide valuable insights that can be instrumental in advancing both chickpea cultivation techniques and strategies for optimizing nitrogen fixation in agricultural systems.
Urine proteome uncover common mechanism between mucopolysaccharidosis type I and II
Authors: Yuan Xiaozhou , Jia Donghao , Wan gefan , Liu kefu , Meng Yan , Duan Jinyan 
Published: December 2023

Abstract
Mucopolysaccharidosis (MPS) type I and II are two types of rare lysosomal storage diseases, which lead to the accumulation of glycosaminoglycans due to the lack of the enzyme alpha-L-iduronidase (IDUA) and iduronate 2-sulfatase (IDS) respectively. There are some similar pathogenic mechanisms and clinical phenotypes but also some specific minute manifestations between these two subtypes. To better understand the similarities in the pathogenesis and clinical symptoms of these two diseases and the mechanism of differential symptoms, we used TMT technology to analyze the differential protein profiles in the urine of MPS I and MPS II patients. We detected the differential proteins of MPS I and MPS II compared with the control group separately. After that, we focused on 227 differentially expressed proteins (DEPs) which showed consistent changes in the urine of both MPS I and MPS II. Parallel reaction monitoring (PRM) analysis verified that up-regulated HEXB and down-regulated HBA1 showed significant difference in the urine of both subtypes. In addition, we found 391 DEPs by comparative analysis of MPS I and MPS II proteomes and found that DHRS2 contributed to the difference between the two subtypes by PRM verification. Therefore, our study demonstrates the value of multi-sample proteomics combined analysis in exploring the similarities and differences of functional proteins between MPS I and MPS II, which provides a new idea for the clinical study of the characteristics of MPS subtypes and the exploration of common treatment options.
Prediction Performance Metrics Considering the Difficulty of Individual Cases
Authors: Kwon Hyunjin , Josephson Colin , Greenberg Matthew , Lee Joon 
Published: December 2023

Abstract
Prediction performance evaluation is an essential step in machine learning model development. Model performance is generally assessed based on the number of correct and incorrect predictions it makes. However, this evaluation metric has a limitation in that it treats all cases equally, regardless of their varying levels of prediction difficulty. In this paper, we propose novel prediction performance metrics considering the prediction difficulty. The novel performance metrics reward models for correct predictions on difficult cases and penalize them for incorrect predictions on easy cases. The prediction difficulty of individual cases is measured using three case difficulty calculation metrics developed by neural networks. We conducted experiments using a variety of datasets and seven machine learning models to compare prediction performance with and without considering the difficulty of individual cases. The experimental results demonstrate that our novel prediction performance metrics enhance the understanding of model performance from various aspects and provide a more detailed explanation of model performance than conventional performance metrics.
Individual-level air pollutants exposure and pregnancy outcomes in patients who underwent assisted reproductive technology: A retrospective longitudinal cohort study
Authors: Fang Miao , Guo Qi , Jiang Cunzhong , Miao Lin , Yang Liyan , Wu Zexi , Yao Xiangyu , Ni Feng , Lin Zhijing , Xu Dexiang 
Published: December 2023

Abstract
Background
The effects of air pollution on the results of assisted reproductive technology (ART) treatment in terms of pregnancy outcomes were inconclusive.
Methods
We performed an individual-level retrospective longitudinal cohort study among 11,968 participants undergoing ART treatment from a general hospital in Hefei, China in 2013-20. Monthly mean concentrations of air pollutants [fine particulate matter (PM2.5), suspended particulate matter (PM10), ozone (O3), sulfur dioxide (SO2), Nitrogen dioxide (NO2) and carbon oxide (CO)] during 3 Periods were obtained from ChinaHighAirPollutants (CHAP) dataset: respective Periods 1, 2 and 3 refer to 90 days, one or two years prior to oocyte retrieval. Multiple logistic regression model was applied to explore the impact of air pollution on four ART results (biochemical pregnancy, clinical pregnancy, pregnancy loss, and live birth).
Results
We observed negative relationships of PM10, PM2.5, CO and SO2 exposures with pregnancy outcomes were more evident during one year exposure (Period 2). An interquartile range increment of ambient PM10, PM2.5, CO, and SO2 exposures during Period 2 was associated with respective decrements of 5.85% (aOR: 0.94, 95% CI: 0.90–0.99), 7.82% (aOR: 0.92, 95% CI: 0.88–0.97), 10.60% (aOR: 0.89, 95% CI: 0.82–0.93) and 12.38% (aOR: 0.88, 95% CI: 0.82–0.93) on clinical pregnancy, while O3 and NO2 showed positive associations. Associations were stronger in patients undergoing frozen embryo transfer, aged < 32 years, with normal BMI, employed status, one embryo transferred, and in warm season.
Conclusions
Our data show that long-term individual-level exposure to the air pollutants of PM10, PM2.5, CO, SO2 but not O3 or NO2, especially in one year exposure before oocyte retrieval, could have a negative impact on ART outcomes.