bluuebunny/crossref_metadata_2025_split huggingface dataset:
The best company cultures are “people first”
Authors: Hiler Kimberly 
Published: September 2023

Purpose
This paper aims to prove that putting people first is a key tenet to successfully engaging with your employees and building a strong company culture. Recognizing the importance of this approach is paramount for HR professionals and company leaders.

Design/methodology/approach
With a people-first approach, employees are placed at the forefront of all business decisions. Intradiem has abided by a people-first philosophy for years, and the company has seen incredible payoff in employee engagement and business success.

Findings
In a business world increasingly characterized by technology and automation, the human element remains as vital as ever. This is especially true in customer service, which is, at its core, a quintessentially “human” exchange.

Originality/value
Appreciation for the value of prioritizing employees is not new, of course. The responsibility of people leaders is to ensure that the people they recruit, hire and train will feel secure, apprecited and valued. Because when they don’t, their performance suffers. That will be reflected in customer satisfaction and, ultimately, business performance.
Sacred Circle: Symbol of Wholeness in Traditional Persian Art and Architecture
Authors: Darabimanesh Fariba 
Published: September 2023

AbstractJung wrote extensively about the archetypal mandala symbol as an expression in many cultures of the centrality and nature of the interplay between human consciousness and divine consciousness. This article investigates—how in Persia, for millennia—the archetypal symbol of the mandala has been widespread in many expressions of the sacred arts. My research outlines the importance of the archetypal mandala symbol in Persian religio‐aesthetic history from the first unearthed stone carvings of Persia's ancient foundations until the more recent, breathtakingly marvellous ceilings of traditional Persian architecture today. From the artistic expressions of first religious beliefs of ancient Persia—Mithraism—and through the development of the Zoroastrian faith until the subsequent rise of Christianity and then Islam, Persian sacred art illustrates the Jungian idea that wholeness sought in the journey of individuation is often expressed through archetypal symbols of circles that articulate basic truths about the divine interplay with humanity.
rcontroll: An R interface for the individual‐based forest dynamics simulator TROLL
Authors: Schmitt Sylvain , Salzet Guillaume , Fischer Fabian Jörg , Maréchaux Isabelle , Chave Jerome 
Published: September 2023

Abstract

A central challenge in ecology is understanding the emergence of patterns as the result of interactions among individuals. Dynamic forest models can provide a fine‐scale description of the ecological, physiological and environmental processes that explain the demography of coexisting tree species. This in turn helps predict changes under future scenarios. However, model accessibility is a major obstacle to a wide use and communication across scientific disciplines and for educational purposes.

Here, we present the R package rcontroll, which provides access to the TROLL forest simulator in the R environment. TROLL is individual‐based and spatially explicit and leverages knowledge of ecology, biogeochemistry and tree ecophysiology through a trait‐based parameterisation. TROLL has been used to simulate carbon fluxes and tree diversity in tropical and subtropical forests and to explore forest resilience to disturbance and environmental changes more generally. rcontroll provides a user‐friendly environment to set up and analyse TROLL simulations with varying community compositions, ecological parameters and climate conditions.

We show how to test parameter sensitivity in TROLL using the rcontroll R package. We also demonstrate the flexibility and ease of use of rcontroll by replicating a previously published study based on the TROLL simulator. Both examples are included with reproducible code documents.

Complex forest simulators are important scientific tools for science and education, and wide access to these tools is an important condition for their adoption. TROLL is designed to address a wide range of ecological and environmental questions, and the new R package rcontroll is designed to be an entry point for TROLL model users.
Daxx knockdown promoted rDNA transcription without impairing H3.3 expression in mouse preimplantation embryos
Authors: Song Sihang , Wang Nan , Huang Xing‐wei , Jiang Qi , Cheng Xiang‐rong , Pang Nan , Lei Lei 
Published: September 2023

AbstractDuring fertilization, DAXX (death domain‐associated protein) mediates histone variant H3.3 incorporation into heterochromatin, which plays an important role in the maintenance of genomic integrity. rDNA, the ribosomal gene, is included in the first wave of gene activation after fertilization. Our and other studies indicated that loss of Daxx disturbs rDNA heterochromatinization and promotes rDNA transcription without change in protein expression of H3.3. However, maternal and zygotic deletion of Daxx impairs blastocyst development. Whether Daxx knockdown affects H3.3 expression and improves the rDNA transcription in preimplantation development has not been reported. In the present study, we injected HA‐labelled H3.3 (H3.3‐HA) into oocytes during ICSI procedure, and detected H3.3 and DAXX by immunofluorescent staining. Then, we knockdowned Daxx and detected the gene expression levels of Daxx, H3.3, 18s and 47s rRNA. We also performed immunofluorescent staining of B23, γH2A and EdU incorporation to demonstrate nuclear structure, DNA damage and replication. We found injection of H3.3‐HA did not impair preimplantation development. Daxx siRNA did not change expression of H3.3 mRNA, and the development of two‐cell embryos and blastocysts, but the overall replication and expression levels of rRNA were increased compared with that in the control group. Finally, knockdown of DAXX did not aggravate the DNA damage but loosened the nucleolus. We concluded that Daxx knockdown promoted DNA replication and rDNA transcription, but did not affect H3.3 expression and subsequent preimplantation development.
Metabolic signature for a dysbiotic microbiome in the female genital tract: A systematic review and meta‐analysis
Authors: Gill Biban , Schwecht Ingrid , Rahman Nuzhat , Dhawan Tushar , Verschoor Chris , Nazli Aisha , Kaushic Charu 
Published: September 2023

AbstractBackgroundThe vaginal microbiome (VMB) is a critical determinant of reproductive health, where a microbial shift towards a dysbiotic environment has implications for susceptibility to, and clinical presentation of sexually transmitted infections (STIs). Metabolomic profiling of the vaginal microenvironment has led to the identification of metabolic responses to clinical conditions of dysbiosis. However, no studies have examined metabolic markers that are common across conditions and can serve as a signature for vaginal dysbiosis.Method of StudyWe have conducted a comprehensive systematic review and meta‐analysis to identify consistently deregulated metabolites along with their impact on host and microbial metabolism during dysbiosis. We employed two complementary approaches including a vote counting analysis for all eligible studies identified in the systematic review, in addition to a meta‐analysis for a subset of studies with sufficient available data. Significantly deregulated metabolites were then selected for pathway enrichment analysis.ResultsOur results revealed a total of 502 altered metabolites reported across 10 dysbiotic conditions from 16 studies. Following a rigorous, collective analysis, six metabolites which were consistently downregulated and could be generalized to all dysbiotic conditions were identified. In addition, five downregulated and one upregulated metabolite was identified from a bacterial vaginosis (BV) focused sub‐analysis. These metabolites have the potential to serve as a metabolic signature for vaginal dysbiosis. Their role in eight altered metabolic pathways indicates a disruption of amino acid, carbohydrate, and energy metabolism during dysbiosis.ConclusionBased on this analysis, we propose a schematic model outlining the common metabolic perturbations associated with vaginal dysbiosis, which can be potential targets for therapeutics and prophylaxis.
Impact of bisphenol A on cell viability and inflammatory cytokine production in human cervical epithelial cells
Authors: Tantengco Ourlad Alzeus G. , Vidal Manuel S. , Bento Giovana Fernanda Cosi , Menon Ramkumar 
Published: September 2023

AbstractProblemAn intact cervix is a barrier that prevents pathogenic bacteria from invading the uterine and amniotic cavity during pregnancy. Its disruption is associated with ascending infection and adverse pregnancy outcomes. This study analyzed the effects of bisphenol A (BPA), a chemical used in plastics manufacturing, on cell death and inflammation in cervical epithelial cells.MethodsEctocervical epithelial (ecto) and endocervical epithelial (endo) cells were treated with 100 ng/mL and 300 ng/mL of BPA for 48 h. The cells were subjected to flow cytometry using annexin V and propidium iodide to determine apoptosis and necrosis, cell cycle analysis, and ELISA to determine the levels of inflammatory cytokines (IL‐6, IL‐8, and IL‐10).ResultsLow‐dose and high‐dose BPA significantly increased the live ecto cell population dose‐dependently. BPA did not have any noticeable effect on cell cycle progression in either cell type. BPA treatment also decreased the apoptotic ecto and endo cell population dose‐dependently. Lastly, high dose BPA significantly increased IL‐6 in ecto and endo cells. However, IL‐8 and IL‐10 were not affected by BPA treatments.ConclusionChemical exposure damage to the cervix can lead to adverse pregnancy outcomes. Our study showed that the BPA concentrations reported in pregnant subjects do not induce cervical cell toxicity . The decrease in apoptosis and increase in live cells may be a compensatory mechanism to preserve the integrity of the cervical epithelial layer.
Editorial: Negative impact of diabetes mellitus and obesity on the prognosis of patients with chronic liver disease—Authors' reply
Authors: Paternostro Rafael , Jachs Mathias , Reiberger Thomas , Mandorfer Mattias 
Published: September 2023

LINKED CONTENTThis article is linked to Paternostro et al papers. To view these articles, visithttps://doi.org/10.1111/apt.17653andhttps://doi.org/10.1111/apt.17673
Socioeconomic inequalities in early child development in children aged under 36 months in South Asia: A systematic review
Authors: Chandrasenage Damith , Markey Oonagh , Johnson William , Haycraft Emma , Griffiths Paula L. 
Published: September 2023

AbstractBackgroundIn South Asia, 89 million children under 5 are at risk of not reaching their developmental potential. Household socioeconomic position (SEP) is a determinant of early child development (ECD). However, synthesised evidence for the association between ECD and SEP in young children in South Asia is not available. Therefore, this review synthesises evidence on the relationship of household SEP with ECD in children under 36 months of age in South Asia.MethodPubMed, Cochrane Library, MEDLINE and Scopus were systematically searched to identify studies from South Asian countries that reported evidence on the association between SEP and ECD. Search terms included items related to motor, cognitive, language and socioemotional development. Study quality was assessed using the QualSyst tool, with three quality levels (high/medium/low), and a narrative review for each ECD outcome was constructed (PROSPERO registration: CRD42019131533).ResultsTwelve of the 950 publications screened met the inclusion criteria (nine from India, two Nepal and one Bangladesh). The majority (n = 10, 83%) reported language development on its own or alongside another ECD outcome. Fewer articles assessed cognitive (n = 6, 50%), motor (n = 7, 58%) or socioemotional development (n = 3, 25%). Higher SEP was associated with better ECD for one third of the associations reported. One ECD outcome (socioemotional development) was negatively associated (with socioeconomic status) based on low quality evidence. Mother's education and family income were the major SEP constructs associated with ECD. One, four and seven studies were rated as having a low, medium and high risk of bias, respectively.ConclusionThis review reveals the scarcity of evidence exploring associations between household SEP and ECD in children under 36 months in South Asia, especially outside of India. Enhancing evidence for associations between ECD and SEP is needed for evidence‐based policy making to reduce developmental delays associated with a disadvantaged SEP in the South Asian region.
Lessons from COP15 on effective scientific engagement in biodiversity policy processes
Authors: Carroll Carlos , Hoban Sean , Ray Justina C. 
Published: September 2023

AbstractThe Kunming–Montreal Global Biodiversity Framework was adopted by parties to the Convention on Biological Diversity in December 2022. The aftermath of these negotiations provides an opportunity to draw lessons as to how ecological and evolutionary science can more effectively inform policy. We examined key challenges that limit effective engagement by scientists in the biodiversity policy process, drawing parallels with analogous challenges within global climate negotiations. Biodiversity is multifaceted, yet represents only one framing for nature's contributions to people, complicating the nexus between evidence and values in development of the framework's targets. Processes generating biodiversity and driving its loss are multiscalar, challenging development of an evidence base for globally standardized targets. We illustrated these challenges by contrasting development of 2 key elements of the framework. The genetic diversity element of the framework's target 4 is directly related to the framework's primary goals, but its complexity required development of novel engagement skills. The target for protected areas was easily communicated but more indirectly related to biodiversity outcomes; evidence from ecological and social science was essential to communicating the context and limitations of this relationship. Scientists can strengthen the effectiveness of global agreements and address challenges arising from complexity, scaling, capacity limitations, and the interplay of science and values, if they can prioritize communication, consensus‐building, and networking skills and engage throughout the process, from development of an evidence base to implementation.
Release of glucose from dissolved and mineral‐bound organic matter by enzymatic hydrolysis
Authors: Lenhardt Katharina R. , Brandt Luise , Poll Christian , Rennert Thilo , Kandeler Ellen 
Published: September 2023

AbstractSorption of dissolved organic matter (DOM) by poorly crystalline minerals during their formation may protect large amounts of carbon in soils from mineralization. We investigated the bioavailability of carbohydrates in DOM and after co‐precipitation with short‐range ordered aluminosilicates. Carbohydrates originated from soil solutions collected in situ at two depths of a Dystric Cambisol, and from litter extracts. Quantification of substrate‐specific degradability was achieved by the addition of β‐glucosidase at an optimal concentration and subsequent determination of glucose release. Depending on DOM composition, 0.6–41.4 mg g−1 C−1 of glucose was enzymatically released from dissolved carbohydrates. Co‐precipitated carbohydrates were partially accessible, resulting in a glucose release of 0.7–5.2 mg g−1 C−1. Restricted enzymatic depolymerization due to co‐precipitation may contribute to accumulation of easily degradable substrates in soils.
Effects of larval crowding on the transcriptome of Drosophila simulans
Authors: Buchner Stephan , Hsu Sheng‐Kai , Nolte Viola , Otte Kathrin A. , Schlötterer Christian 
Published: September 2023

AbstractLarval crowding is one common ecological stressor for many insect species. In Drosophila, high larval density alters multiple widely‐studied phenotypes including life‐history traits, morphology and behavior. Nevertheless, we still miss a holistic view of the full range of phenotypic changes and the underlying molecular mechanisms. In this study, we analyzed the adult transcriptomes of high and low larval density fly cohorts, and highlighted the molecular basis of the plastic traits. Increased cellular energy metabolism and locomotion, along with reduced reproductive investment, are key responses to high larval density. Moreover, we compared the expression changes among cohorts with different developmental delays caused by larval crowding. The majority of genes induced by larval crowding showed the strongest expression alterations in cohorts with intermediate delay. Furthermore, linear expression changes were observed in genes related to nutrition and detoxification. Comparing different high‐density cohorts could provide insights into the varied responses to distinct larval crowding‐induced stresses such as space competition, food degradation and waste accumulation.
Rapid weight change as a predictor of disability among community‐dwelling Japanese older adults
Authors: Tsubota‐Utsugi Megumi , Tanno Kozo , Takahashi Naomi , Onoda Toshiyuki , Yonekura Yuki , Ohsawa Masaki , Takahashi Shuko , Kuribayashi Toru , Itabashi Ryo , Tanaka Fumitaka , Asahi Koichi , Omama Shinichi , Ogasawara Kuniaki , Ishigaki Yasushi , Takahashi Fumiaki , Soma Akemi , Takanashi Nobuyuki , Sakata Kiyomi , Ohkubo Takayoshi , Okayama Akira 
Published: September 2023

AimTo fill the knowledge gap regarding weight change and the onset of disability in community‐dwelling Japanese older adults, we investigated the potential effects of rapid weight change on disability risk as defined by Japan's long‐term care insurance (LTCI) system.MethodsWe analyzed data from a longitudinal study of 10 375 community‐dwelling older Japanese adults (≥65 years) who were not LTCI needs certified at baseline and joined the study from 2002 to 2005. Weight change (percentage) was calculated by subtracting participants' weight in the previous year from that measured during a physical examination at study commencement. The five weight‐change categories ranged from sizable weight loss (≤ −8.0%) to sizable weight gain (≥ +8.0%). Disability was defined according to LTCI certifications at follow‐up. Hazard ratios (HRs) and 95% confidence intervals were calculated for new‐onset disability using a Cox proportional hazards model that fitted the proportional subdistribution hazards regression model with weights for competing risks of death.ResultsDuring the mean 10.5‐year follow‐up, 2994 participants developed a disability. Sizable weight loss (HR [95% confidence intervals], 1.41 [1.17–1.71]) and weight loss (1.20 [1.05–1.36]) were significant predictors of disability onset. Sizable weight gain (1.45 [1.07–1.97]) corresponded to severe disability. Stratified analyses by lifestyle and initial body mass index categories revealed more pronounced associations between weight change and disability risk in the unhealthy lifestyle and below initial normal body mass index groups.ConclusionsRapid and sizable weight gain could be additional criteria for disability risk in older adults. Geriatr Gerontol Int 2023; 23: 809–816.
Preclinical development and characterization of a human plasma‐derived high‐purity factor X concentrate for therapeutic use
Authors: Lloyd Joanne , Feldman Peter 
Published: September 2023

AbstractIntroductionHereditary factor X deficiency is a rare bleeding disorder, with limited treatment options. This paper describes the approach to pre‐clinical development and characterization of a high‐purity plasma‐derived factor X concentrate, to achieve orphan drug marketing authorization for the treatment of hereditary factor X deficiency.MethodsA chromatographic process was developed, to purify factor X from human plasma for fractionation. The product was characterized using in vitro, in vivo and ex vivo tests for potency, purity, thrombogenicity, immunogenicity, toxicity and stability.ResultsThe production process complied with good pharmaceutical manufacturing practice. It achieved 6000‐fold purification and 100‐fold concentration of the factor X protein compared to human plasma. The factor X protein was 94%–96% pure. Other residual plasma proteins were well below levels in plasma, minimizing potential interference in hemostasis after therapeutic administration. Effective virus‐reduction during manufacture, and the absence of thrombogenicity, toxicity and immunogenic potential were confirmed, addressing the main safety concerns historically associated with plasma‐derived therapeutics. The freeze‐dried product remained stable between +2°C and +30°C for at least three years. After reconstitution with water for injections, the factor X activity was maintained for at least 48 h at +18°C to +22°C.ConclusionTargeted pre‐clinical development of the first highly‐purified concentrate to treat hereditary factor X deficiency is described. Following international guidelines for nonclinical safety testing, particular strategies were adopted for unmodified products derived from human blood plasma. This approach may also be relevant to the development of other ultra‐orphan medicinal products.
The in vitro effect of anticoagulant agents on coagulation and fibrinolysis in the presence of emicizumab in the plasmas from patients with haemophilia A
Authors: Onishi Tomoko , Harada Suguru , Shimo Hanako , Tashiro Yoshihito , Soeda Tetsuhiro , Nogami Keiji 
Published: September 2023

AbstractIntroductionEmicizumab is used as hemostatic prophylaxis for patients with hemophilia A (PwHA), irrespective of the presence of inhibitors. Although bacterial infection can lead to a procoagulant state, there is limited information on coagulation and fibrinolysis potentials in emicizumab‐treated PwHA and on the use of anticoagulants in such cases.AimWe examined whether anticoagulants affect the coagulation and fibrinolysis potentials in plasma from PwHA spiked with emicizumab.MethodsPlasma from PwHA was in vitro supplemented with emicizumab (50 μg/mL; emi‐plasma) and anticoagulants (recombinant thrombomodulin (rTM), nafamostat mesylate (NM), unfractionated heparin (UFH), or low‐molecular‐weight heparin (LMH)). PwHA plasma spiked with rFVIII (1 IU/mL) was used as a reference (ref‐plasma). The coagulation and fibrinolysis potentials in plasma was measured by thrombin and plasmin generation assay (T/P‐GA) and clot‐fibrinolysis waveform analysis (CFWA).ResultsIn T/P‐GA and CFWA, coagulation potentials (maximum coagulation velocity; |min1|, and peak thrombin; Th‐Peak) in plasma rose with increasing concentrations of emicizumab and rFVIII, but fibrinolytic potentials (peak plasmin; Plm‐Peak, and maximum fibrinolytic velocity; |FL‐min1|) remained unchanged. Adding rTM, NM, and UFH to emi‐plasma suppressed coagulation and fibrinolysis potentials, similar to ref‐plasma. Regarding the heparin, UFH and LMH inhibited the improved coagulation in emi‐plasma. UFH inhibited fibrinolysis as well, but LMH did not.ConclusionsAnticoagulants could exhibit the inhibitory effects on the coagulation and fibrinolysis potentials in plasma from PwHA spiked with emicizumab, similar to those in normal plasma.