bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic value of oxygen inhalation therapy for simple nocturnal hypoxemia in COPD: a meta-analysis
Authors: Sun Ling , Chang Ya-Fei , Wang Yun-Fei , Li Xiang-Rong , Ran Xian-Zhong , Xie Quan-Xin 
Published: September 2023

Abstract
Objective
The effect of oxygen therapy on the prognosis of chronic obstructive pulmonary disease (COPD) with nocturnal hypoxemia (NOD) has been controversial. Therefore, this study systematically evaluated the relevant literature and included it into randomized controlled studies for meta-analysis to evaluate the efficacy and prognosis.
Methods
We searched PubMed, EMBASE, web of science, Cochrane, China HowNet and Wanfang database for the literature on the prognosis of COPD patients with simple nod from the establishment of the database to June 30, 2022. The outcome indicators were death and aggravation of the disease. The efficacy evaluation measures were pulmonary function and arterial blood gas results. The publication bias and heterogeneity of the included studies were evaluated.
Results
A total of 621 patients from 5 studies were included in this meta-analysis, and there was no publication bias in the included studies. The total mortality of long-term oxygen therapy (LTOT) in COPD patients with simple nod in oxygen therapy group (RR = 1.04; 95% CI: 0.82–1.34, P = 0.73), mortality (RR = 0.85; 95% CI: 0.62–1.16, P = 0.31), risk of progression to LTOT events (RR = 1.07; 95% CI: 0.76–1.51, P = 0.68). PaO2 in patients with COPD and simple nod in oxygen therapy group was higher than that in non-oxygen therapy group (mean difference (MD) = 13.47; 95% CI: 3.49–23.46, P = 0.0008), the decrease of PaCO2 level was not statistically significant (MD = -10.05; 95% CI: -26.36-6.27, P = 0.23).
Conclusion
Oxygen therapy can improve the prognosis of blood oxygen partial pressure in COPD patients with simple nod, but oxygen therapy has no significant effect on the survival rate, controlling the progression of the disease to LTOT and reducing the partial pressure of carbon dioxide.
Gene expression profiles of endothelium, microglia and oligodendrocytes in hippocampus of post-stroke depression rat at single cell resolution
Authors: Li Cai , Li Wentao , Wei Wenbin , Gao Han , Zhao Yanqing , Zhang Lingling , Ling Li , Shen Yifen , Shen Yihang , Shen Hao 
Published: September 2023

Abstract

Post-stroke depression (PSD) is a common but severe mental complication after stroke. However, the cellular and molecular understanding of PSD is still yet to be illustrated. In current study, we prepared PSD rat model (MD) via unilateral middle cerebral artery occlusion (MCAO) and chronic stress stimulation (DEPR), and isolated hippocampal tissues for single cell sequencing of 10x Genomics Chromium. First, we determined the presence of the increased cell population of endothelium and microglia and the compromised oligodendrocytes in MD compared to NC, MCAO and DEPR. The enriched functions of highly variable genes (HVGs) of endothelium and microglia suggested a reinforced blood-brain barrier in MD. Next, cell clusters of endothelium, microglia and oligodendrocytes were individually analyzed, and the subtypes with distinct functions were identified. The genotype of PSD displayed more similarity with DEPR compared to MCAO and NC. For endothelium, the absence of cell differentiation, but robust proliferation and fibrosis instead were observed in MD. For microglia, multiple subpopulations showed the superimposition of neurotoxic and neuroprotective functions, and DEPR could enlarge the effect of microglia in MCAO. For oligodendrocytes, the one for demyelination were elevated in DEPR and MD, while the one for remyelination were robust in MCAO, and the oligodendrocytes undergoing demyelination were processed via apoptosis, autophagy and ferroptosis manner. Finally, we also observed that the intercellular crosstalk among these three cells were largely elevated in MACO but compromised in DEPR, whereas was intermediate between them in MD, and depression and stroke could both activate the inflammation reaction but through different signals. Taken together, this study characterized the single cell expression profile of hippocampal PSD, and unmask the differential expressed genes of endothelium, microglia and oligodendrocytes, emphasizing the crosstalk among them to provide theoretical basis for the in-depth mechanism research and drug therapy of PSD.
Mating practices, breeding objectives and trait preferences of village chicken farmers in Limpopo Province, South Africa
Authors: Rasekgoalo Tinah Christinah , Raphulu Thomas , Sebei Phokgedi Julius , Hlongwane Nompilo Lucia , Matelele Tlou Cornelia , Chokoe Tlou Caswell , Muchadeyi Farai , Dzomba Edgar , Hadebe Khanyisile 
Published: September 2023

Abstract

Sustainable production improvement strategies suited for the resource-limited farmers are sought to replace the misaligned crossbreeding strategies that threaten adaptable genetic resources. This study will contribute towards bridging the knowledge gap that exists on the potential of the village chicken production system and the breeding practices applied by rural farmers in managing their flocks. A survey study on the 55 indigenous chicken farmers was conducted in Sekhukhune (n = 33) and Capricorn (n = 22) Districts of Limpopo Province with the objective to characterise the production environments in order to better understand the traditional mating and selection practices and to identify farmer breeding objectives and trait preferences. Farmers were interviewed using a structured questionnaire to acquire general household information, breeding and flock management, purpose of keeping chickens and traits of interest to the farmers. Fifty percent of the respondents were household heads, aged ≥ 61 (49.06%). Majority (96.36%) of the farmers owned at least one cock of local breed (88.89%), that are hatched and grown in the house (88.46%). All farmers practised uncontrolled breeding. Farmers keep chickens for meat consumption (40.35%), egg consumption (19.30%), passion (19.30%), and income (16.67%). Plumage colour (25.97%), mothering ability (28.57%), and body size (23.38%) were the traits considered in selecting hens for breeding purpose while body size (50.00%) and plumage colour (41.38%) were used in the selection of cocks. The study highlights the village chicken production potential and can aid in providing recommendations for improvement strategies that are suited for the management and breeding preferences of the farmers.
A self-directed digital exercise program for hip osteoarthritis (“My Hip Exercise”): Protocol for a randomised controlled trial
Authors: Bennell Kim L , Nelligan Rachel K , Hall Michelle , Stratulate Sarah , McManus Fiona , Lamb Karen , Marlow Jennifer , Hinman Rana S 
Published: September 2023

Abstract
Background: Hip osteoarthritis (OA) is a leading global cause of chronic pain and disability. Given there is no cure for OA, patient self management is vital with education and exercise being core recommended treatments. However, there is under-utilisation of these treatments due to a range of clinician and patient factors. Innovative service models that increase patient accessibility to such treatments and provide support to engage are needed. This study primarily aims to determine the effects of a self-directed digital exercise intervention comprising online education and exercise supported by a mobile app to facilitate adherence on the primary outcomes of changes in hip pain during walking and patient-reported physical function at 24-weeks when compared to online education control for people with hip OA.
Methods: We will conduct a two-arm, superiority parallel[s1] [KB2] -design, randomised controlled trial involving 182 community volunteers aged 45 years and over, with painful hip OA. After completing the baseline assessment, participants will be randomly assigned to either: i) digital exercise intervention; or ii) digital education (control). Participants randomised to the intervention group will have access to a website that provides information about hip OA and its management, advice about increasing their physical activity levels, a 24-week lower limb strength exercise program to be undertaken at home three times per week, and a mobile app to reinforce home exercise program adherence. Participants in the control group will have access to a website containing only information about hip OA and its management. All participants will be reassessed at 24 weeks after randomisation. Primary outcomes are severity of hip pain while walking using an 11-point numeric rating scale and physical function using the Western Ontario and McMaster Universities Osteoarthritis Index subscale. Secondary outcomes are the Hip dysfunction and Osteoarthritis Outcome Score subscales of pain, hip-related quality of life, and function, sports and recreational activities; global change in hip condition; health-related quality of life; measures of physical activity levels; fear of movement; self efficacy for pain and for exercise; and use of oral pain medications.
Discussion: Innovative and scalable approaches to OA education, physical activity, and exercise are required in order to improve exercise participation/engagement and mitigate physical inactivity in the hip OA population. This will help minimise the burden of this major public health issue on individuals and society.
Trial registration: Australia New Zealand Clinical Trials Registry (ACTRN12622001533785)
Ouabain increases neuronal differentiation of hippocampal neural precursor cells
Running title: Role of Ouabain in neurogenesis
Authors: Kawamoto Elisa , Orellana Ana , Port´s Natacha , Lima Larissa , Leite Jacqueline , Andreotti Diana , Kinoshita Paula , Anjos Leticia , Neto João Machado , Scavone Cristoforo 
Published: September 2023

Abstract
It has been shown that Ouabain (OUA), a cardiotonic steroid, can bind to Na,K-ATPase (NKA) and trigger many signaling pathways in adult rat hippocampus such as cAMP response element-binding protein (CREB), Wnt/β-catenin and NF-κB in neurons, increasing Brain-derived neurotrophic factor (BDNF) release, neuronal dendritic branching and leading to a spatial long-term memory improvement. This study aimed to verify whether OUA affects neural precursor cells in the dentate gyrus, which is the main site of adult hippocampal neurogenesis. Primary cultures of neural precursor cells (NPCs) were made from newborn Wistar rat dentate gyri. These results suggest that OUA increases Neurod2 transcription in NPCs, but persistently decreases the release of NT3. Furthermore, OUA increased BDNF and nerve growth factor (NGF) levels and the percentage of generated neurons. These neurons also had a more developed dendritic arbor than the control group. In conclusion, 10 nM OUA increased NPCs differentiation into neurons, suggesting a new role for NKA in neurogenesis.
New rabies viral resources for multi-scale neural circuit mapping
Authors: Xu Xiangmin , Bouin Alexis , Wu Ginny , Koyuncu Orkide , Ye Qiao , Kim Keun-Young , Wu Michele , Tong Liqi , Chen Luija , Phan Sebastien , Mackey Mason , Ramachandra Ranjan , Ellisman Mark , Holmes Todd , Semler Bert 
Published: September 2023

Abstract
Comparisons and linkage between multiple imaging scales are essential for neural circuit connectomics. Here we report 20 new recombinant rabies virus (RV) vectors that we have developed for multi-scale and multi-modal neural circuit mapping tools. Our new RV tools for mesoscale imaging express a range of improved fluorescent proteins. Further refinements target specific neuronal subcellular locations of interest. We demonstrate the discovery power of these new tools: the detection of detailed microstructural changes of rabies-labeled neurons in aging and Alzheimer's disease mouse models, live imaging of neuronal activities using calcium indicators, and automated measurement of infected neurons. RVs that encode GFP and ferritin as electron microscopy (EM) and fluorescence microscopy reporters are used for dual use EM and mesoscale imaging. These new viral variants significantly expand the scale and power of rabies virus-mediated neural labeling and circuit mapping across multiple imaging scales in health and disease.
Enhancing Patient-Clinician Collaboration During Treatment Decision-Making: Study Protocol for a Community-Engaged, Mixed Method Hybrid Type 1 Trial of Collaborative Decision Skills Training (CDST) for Veterans with Psychosis
Authors: Treichler Emily B.H. , McBride Lauren E. , Gomez Elissa , Jain Joanna , Seaton Sydney , Yu Kasey E. , Oakes David , Perivoliotis Dimitri , Girard Vanessa , Reznik Samantha , Salyers Michelle P. , Thomas Michael L. , Spaulding William D. , Granholm Eric L. , Rabin Borsika A. , Light Gregory A. 
Published: September 2023

Abstract
Background
Patient participation in treatment decision making is a pillar of recovery-oriented care and is associated with improvements in empowerment and well-being. Although demand for increased involvement in treatment decision-making is high among Veterans with serious mental illness, rates of involvement are low. Collaborative Decision Skills Training (CDST) is a recovery-oriented, skills-based intervention designed to support meaningful patient participation in treatment decision making. An open trial among Veterans with psychosis supported CDST’s feasibility and demonstrated preliminary indications of effectiveness. A randomized control trial (RCT) is needed to test CDST’s effectiveness in comparison to an active control and further evaluate implementation feasibility.
Methods
The planned RCT is a hybrid type 1 trial, which will use mixed methods to systematically evaluate the effectiveness and implementation feasibility of CDST among Veterans participating in a VA Psychosocial Rehabilitation and Recovery Center (PRRC) in Southern California. The first aim is to assess the effectiveness of CDST in comparison to the active control via the primary outcome, collaborative decision-making behavior during usual care appointments between Veterans and their VA mental health clinicians, and secondary outcomes (i.e., treatment engagement, satisfaction, and outcome). The second aim is to characterize the implementation feasibility of CDST within the VA PRRC using the Practical Robust Implementation and Sustainability Model framework, including barriers and facilitators within the PRRC context to support future implementation.
Discussion
If CDST is found to be effective and feasible, implementation determinants gathered throughout the study can be used to ensure the sustained and successful implementation at this PRRC and other PRRCs and similar settings nationally.
Trial registration: ClinicalTrials.gov Identifier: NCT04324944. Registered on March 27th, 2020. Trial registration data can be found in Appendix 1.
Total nasal reconstruction in irradiated tissue, a new hope
Authors: DUPRET-BORIES Agnès , Chabrillac Emilien , Poissonnet Valentine , Nolens Gregory , Henriet Valentin , Bouakaz Islam , Sarini Jérome , Grossin David , Riviere Louis-David , Vergez Sébastien , Vairel Benjamin 
Published: September 2023

Abstract
The 16th century expression "plain as the nose on one’s face" by Rabelais, used to refer to the obvious, reflects the paramount importance of the nose in a person's social life. However, total nasal reconstruction remains an unsolved surgical challenge. Through the case of a 50-year-old patient who lost her nose after cancer treatment, the authors present a total nasal reconstruction in two surgical steps using a novel and fully integrated 3D-printed biomaterial. In light of the surgical outcomes and the evolution of the patient's quality of life, this novel technique is a source of hope for many patients.
Control of meltpool shape in laser welding
Authors: Suder Wojciech , Chen Xin , Sierra David Rico , Chen Guangyu , Wainwright James , Rajamudili Kuladeep , Pardal Goncalo Rodrigues , Williams Stewart 
Published: September 2023

Abstract

In laser welding, the achievement of high productivity and precision is a relatively easy task; however, it is not always obvious how to achieve sound welds without defects. The localised laser energy promotes narrow meltpools with steep thermal gradients, additionally agitated by the vapour plume, which can potentially lead to many instabilities and defects. In the past years, there have been many techniques demonstrated on how to improve the quality and tolerance of laser welding, such as wobble welding or hybrid processes, but to utilise the full potential of lasers we need to understand how to tailor the laser energy to meet the process and material requirements. Understanding and controlling the melt flow is one of the most important aspects in laser welding. In this work the outcome of an extensive research programme on understanding the meltpool dynamics and control of bead shape in laser welding is discussed. The results of instrumented experimentation, supported by computational fluid dynamic modelling give insight into the fundamental aspects of meltpool formation, flow direction, feedstock melting and the likelihood of defect formation in the material upon laser interaction. The work contributes to a better understanding of the existing processes, as well as development of new range of process regimes with higher process stability, improved efficiency and higher productivity than standard laser welding. Several examples including, ultra-stable keyhole welding and wobble welding and a highly efficient laser wire melting are demonstrated. In addition, the authors present a new welding process, derived from a new concept of the meltpool flow and shape control by dynamic beam shaping. The new process has proven to have many potential advantages in welding, cladding and repair applications.
Low Toxicity Liquid Phase Microextraction Using Deep Eutectic Solvent-Based Ferrofluid for Enrichment Polycyclic Aromatic Hydrocarbon in Environmental Samples
Authors: Sazali Nur Hidayah , Miskam Mazidatulakmam , Suah Faiz Bukhari Mohd , Rahim Nurul Yani 
Published: September 2023

Abstract
An environmental friendly ferrofluid incorporating hydrophobic deep eutectic solvents derived from fatty acids, specifically caprylic acid, pelargonic acid, capric acid, and lauric acid have been developed. The fundamental physiochemical properties of the synthesised deep eutectic solvents ferrofluid were determined using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and scanning electron microscopy (SEM). Later, they will be used as alternative adsorbents in ferrofluid liquid-phase microextraction. Five polycyclic aromatic hydrocarbons (PAHs) were determined simultaneously using gas chromatography with a flame ionisation detector. The most important extraction parameters were optimised, and the procedure was validated. The selected experimental variables were then optimised using Box-Behnken design (BBD). The developed method is also assessed by Analytical Eco-Scale and Analytical Greenness for greenness method evaluation. Under the optimal conditions, the presented method demonstrated wide linear ranges of 0.005–5 µg mL− 1 for all PAHs. The limit of detections (LODs) and quantifications (LOQs) of PAHs varied from 0.4–1.70 ng mL− 1 and 1.33–5.67 ng mL− 1, respectively. The extraction recoveries of spiked samples for FF-LPME ranged from 75.78 to 118.65% with RSD < 15%. The Analytical Eco-scale and Analytical GREENness Metric Approach System (AGREE) measured 92% and 0.64, respectively. The optimised process successfully delivered an environmentally friendly adsorbent, demonstrating a highly promising approach for extracting PAHs from various environmental samples.
BRCA1 mutation promotes sprouting angiogenesis in inflammatory cancer-associated fibroblast of triple-negative breast cancer
Authors: Fang Sungsoon , Lee Chae Min , Hwang Yeseong , Jeong Jae Woong , Kim Minki , Lee Janghee , Bae Soong June , Ahn Sung Gwe 
Published: September 2023

Abstract
Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with inferior outcomes owing to its low treatment response and high invasiveness. Based on abundant cancer-associated fibroblasts (CAFs) and frequent mutation of breast cancer-associated 1 (BRCA1) in TNBC, the characteristics of CAFs in TNBC patients with BRCA1 mutation compared to wild-type BRCA1 were investigated using single-cell analysis. Intriguingly, we observed a dominant presence of inflammatory CAFs (iCAFs) in BRCA1 mutation compared to the wild-type BRCA1 TNBC patients. iCAFs in patients with BRCA1 mutation exhibited strong signaling to endothelial cells (ECs) clusters, including chemokine (C-X-C motif) ligand 1 (CXCL) and vascular endothelial growth factor (VEGF). During CXCL signaling, the atypical chemokine receptor 1 (ACKR1) mainly interacts with CXCL family members in tumor endothelial cells (TECs). ACKR1-high TECs also showed high expression levels of angiogenesis related genes, such as ANGPT2, MMP1 and SELE, which might lead to EC migration. Furthermore, iCAFs showed VEGF signals for FLT1 and KDR in TECs, which showed high co-expression with tip cell marker genes, including ZEB1 and MAFF, involved in sprouting angiogenesis. Moreover, BRCA1 mutation patient with relatively abundant iCAFs and tip cell gene expression, exhibited a limited response to neoadjuvant chemotherapy, including cisplatin and bevacizumab. Importantly, our study observed the intricate link between iCAFs-mediated angiogenesis and chemoresistance in TNBC with BRCA1 mutation.
Transcriptome analysis of fruit development and identification of key transcription factors related to anthocyanin pathway in cultivated octoploid strawberry
Authors: Wang Baotian , Zhang Chao , Li Jiqi , Li Xue , Mao Jianxin , Wang Yan , Zhang Zhihong 
Published: September 2023

Abstract

The cultivated strawberry is a vital economic crop grown extensively worldwide. Recently, there has been more focus on breeding strawberry cultivars that produce high-quality fruit. Anthocyanin is a crucial determinant of the quality of strawberries. A transcriptomes analysis of fruits at various developmental stages of the 'Yanli' cultivar was performed to examine the mechanisms of anthocyanin accumulation in the process of fruit development of cultivated strawberries. We screened 33,617 differentially expressed genes whose average expression value was more significant than 2 in at least one sample. We classified these differentially expressed genes into nine categories based on k-mean clustering analysis. Furthermore, we recognized that anthocyanin synthesis-related genes’ promoter regions had a high consistency of conserved motifs with a similar expression pattern. By conducting a correlation analysis between seven anthocyanin pathway genes and various transcription factors, we identified 560 transcription factors that might involve in the anthocyanin biosynthesis pathway. In summary, we can identify a more significant number of prospective transcription factors in the anthocyanin pathway based on the haplotype-resolved genome of ‘Yanli’.