bluuebunny/crossref_metadata_2025_split huggingface dataset:
Horizontal gene transfer shapes pathogenic bacteria in multiple sclerosis
Authors: Yamamura Takashi , Takewaki Daiki , Kiguchi Yuya , Masuoka Hiroaki , Manu Mallahalli , Raveney Ben , Narushima Seiko , Kurokawa Rina , Ogata Yusuke , Kimura Yukio , Sato Noriko , Ozawa Yusuke , Yagishita Sosuke , Araki Toshiyuki , Miyake Sachiko , Sato Wakiro , Suda Wataru 
Published: December 2023

Abstract
Multiple sclerosis (MS) is an autoimmune demyelinating disease influenced by environmental factors. Except during relapses, baseline neurological status is generally stable in the early stage, whereas progressive deterioration may occur silently. The progressive disease form (secondary progressive MS; SPMS) characterised by both neuroinflammation and neurodegeneration differs significantly from the non-progressive form in microbiome profiles1 2 3. After confirming an increased abundance of gut bacterium “Tyzzerella nexilis” in SPMS, the role of T.nexilis in progressive MS was studied. The strain-level analysis based on long-read metagenomics identified a distinct cluster of T.nexilis highly enriched in SPMS. T.nexilis strains in this novel cluster were characterised by an incredible number of mobile genetic elements (MGEs) and the absence of defence systems against MGEs. Mono-colonisation with this MGEs-enriched T.nexilis strain made germ-free mice more susceptible to induction of experimental autoimmune encephalomyelitis. The pathogenicity of this strain was mediated by TLR5 stimulation by flagella encoded on MGEs. Moreover, this T.nexilis strain was thought to have potentials of causing neurodegeneration, because of its ability to produce reduced sulphur compounds encoded on MGEs. Such a horizontal gene transfer, causing functional diversity beyond existing bacterial taxonomy, may have causal implications in chronic disorders influenced by gut microbiome.
Primary Intra-orbital Hydatid cyst: a case report of a rare cause of Exophthalmos
Authors: Debela Asrat Sime , Abore Kibruyisfaw Weldeab , Worke Alemayehu Bedane , Wendimagegn Selamawit Tadesse 
Published: December 2023

Abstract
Background: Hydatid disease is a tapeworm parasitic infection caused by Echinococcus granulosus that commonly affects the liver and lungs during its life cycle in the human body. Orbital involvement is a very rare occurrence and isolated orbital Echinococcosis is extremely rare.
Case presentation: A 60-year-old female Eritrean woman presented with a 3 month history of painless protrusion and decreased vision of the left eye. Physical examination showed significantly decreased visual acuity of the left eye, left eye proptosis, and papilledema. Laboratory investigations were normal and orbital CT scan examination was suggestive of orbital hydatid cyst. After a two week course of oral albendazole, a medial anterior orbitotomy and cyst excision were performed. Post-operative biopsy was conclusive of an orbital hydatid cyst. She continued taking oral albendazole 400mg twice daily. On her postoperative follow-up, she had mild lid swelling and adduction deficit. There was significant improvement of the lid swelling and disc edema on the 21st postoperative day but the gain on the adduction deficit was minimal. Subsequent follow-up of the patient was not possible since the patient relocated back to Eritrea.
Conclusion: Orbital hydatid cyst is an extremely rare presentation of human Echinococcus granulosus infection. However, it should be considered one of the differential diagnoses among patients living in an endemic area and presenting with proptosis of the eye. A preoperative course of anthelminthic followed by surgical excision of the cyst is the definitive management.
Rhizobium qitaiense sp. nov. and Mycoplana qihuense sp. nov. isolated from chickpea nodules
Authors: Shen Hongling , Hu Fengxiao , Liang Hongli , Luo Xiaoxia , Wan Chuanxing 
Published: December 2023

Abstract
Two symbiotic nitrogen-fixing bacteria (TRM95796Tand TRM96650T) isolated from effective root nodules of chickpeas were characterised to clarify their taxonomic status using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences, single-copy genes and core-proteome average amino acid identity (cpAAI) indicated that strain TRM95796T was grouped as a separate clade closely related to Rhizobium setariae KVB221T(97.23%), and strain TRM96650T was closely related to Mycoplana. azooxidifex Po 20/26T (97.5%). The results of both the analyses of the concatenated recA atpD and glnII genes and whose genomes revealed that strains TRM95796T was related to two clusters formed by the type strains of the closely related species belonging to Rhizobium, in congruence with the 16S rRNA gene phylogeny. Strain TRM96650T was related to two clusters formed by the type strains of the closely related species belonging to Mycoplana. The OrthoANI values between TRM95796T, and the type strains of the available Rhizobium species were 75.9% for R. setariae KVB221T, 75.8% for R. alvei TNR-22T, which were below the threshold of 95–96% for delineation of bacterial species. The OrthoANI values between TRM96647T, and the type strains of the available Mycoplana species were 90.8% for Mycoplana azooxidifex Po 20/26T, and 82.4% for M. subbaraonis JC85T, which were below the threshold of 95–96% for delineation of bacterial species. The dDDH values of the three novel species with the most closely related species were 18.3–42.8%. Based on the genomic, phenotypic and phylogenetic analyses, strains TRM95796T and TRM96650T are suggested to represent two new species of the genus Rhizobium and Mycoplana, whose names are proposed as Rhizobium qitaiense sp. nov., Mycoplana qihuense sp. nov. The type strains are TRM95796T (= GDMCC 1.3495T = JCM 35985T) and TRM96650T (= GDMCC 1.34953T = JCM35987T), respectively.
Obesity and Underweight in Children Who Undergo Catheterization for Congenital Heart Disease: A Retrospective Study
Authors: Anagnostopoulou Andriana , Eleftherakis Nikolaos , Karanasios Evangelos 
Published: December 2023

Abstract
Background
Τhe number of overweight and obesity in the pediatric population has increased dramatically, a trend that is followed by a corresponding increase in the pediatric population with congenital heart disease. Notwithstanding, children with congenital heart disease seem to follow abnormal growth patterns. The aim of the present study is to determine the prevalence of obesity and underweight in children who undergo cardiac catheterization
Methodology
This is a retrospective study conducted in the cardiac catheterization laboratory of a large pediatric hospital in the years 2011–2019. The children’s anthropometric data were collected and the body mass index was calculated, according to the Centers for Diseases Control and Prevention definition. The chi -square test was used to identify any associations between catheterization, heart disease, body habitus, sex and age groups, after which a one way analysis of variance was performed to investigate the effects of the above variables on obesity.
Results
The participants included 378 children from 2 to19 years old who underwent cardiac catheterization in the years 2011–2019. Cumulatively, 18.7% of the children in the study were underweight, 54.76% were normal weight, 11.64% were overweight and 14.81% were children classified as with obesity. Overall, 26.45% of the children weighed more than the 85th centile. The Kolmogorov Smirnoff test was used to test form normality and only the height in cms had a normal distribution.
Children with acyanotic heart disease had a median age of 8 years old (IQR 6 years). The percentages of underweight were 16.6%, the ones with healthy weight 53.58%, 12.08% overweight and 17.47% with obesity. Children with cyanotic heart disease had a median age 6 years (IQR 6) while the respective percentages of underweight, healthy weight, overweight and with obesity were 27.7%, 56.63%, 9.05% and 6.02%. These differences were statistically significant. However, the children who underwent a catheterization for other causes had a median age of 8 years old (IQR 8) and their percentages of underweight were 13.3%, the ones with healthy weight 60% and the ones with overweight or obesity 13.3% each.
Conclusions
There is a significant discrepancy in the rate of obesity between the children with acyanotic and cyanotic heart disease. However, the differences between the children with acyanotic heart disease and children who underwent cardiac catheterization for other causes is not statistically significant.
Effect of internal barriers on innovation performance in embryonic SMEs in Malaysia
Authors: Hasliza Abdul Halim  , Tarnima Warda Andalib  , Noor Hazlina Ahmad  , T. Ramayah  
Published: December 2023

Manufacturing small and medium enterprises (SMEs) need to propel their creativity and innovativeness to enhance their innovation performance in the competitive and fast-changing environment. The current environment has transformed the internal working pattern, structure, and communication system to survive the rough business ecosphere. Consequently, SMEs encountered innumerable barriers to achieve the innovation performance and the SMEs should recognise the internal barriers so that they are able to accelerate their innovation performance. Therefore, this study aims at examining the internal barriers namely constraint in financial resources, constraint in technology, and constraint in human resources that hamper the innovation performance among the Malaysian manufacturing SMEs. This study embarked on two phases of data collection. First phase includes a series of semi-structured interviews with 15 participants from 5 SMEs via snowballing technique to gather their insights on internal barriers that impede innovation performance. For second phase, the survey method was adopted, and 217 SMEs responded to the survey. All the responses were analysed using SmartPLS technique. The interview findings demonstrate that all the internal constraints are indeed important for SMEs to grasp and serve as the obstacles for innovation performance. Nevertheless, from the quantitative analysis, only financial constraints serve as the significant barrier that impede innovation performance. To summarise, this research produces insights on decisive proof regarding internal barriers that inhibit the manufacturing SMEs to achieve innovation performance.
Comprehensive Characterization and In vitro Evaluation of a Novel
POQCL Drug Delivery System
Authors: Tiwari Chanchal , Singh Arjun , Kumar Dharmendra 
Published: December 2023

Aim:
Formulation and evaluation of the POQCL drug delivery system.

Background:
One of the major barriers in the formulation of dosage forms is the poor solubility of
the drug. BCS class IV drugs are having a problem with pharmacokinetics or reaching the site of
action. Poor water-soluble drugs of BCS class IV obstruct drug bioavailability and decrease their
pharmaceutical development. An attempt has been made in this work to deliver the BCS class IV
drug into a novel carrier dosage form i.e., liposomes using a novel lipid.

Objective:
Formulation of the POQCL drug delivery system. Characterization by average particle
size, surface morphological analysis, % drug entrapment, drug loading, in vitro study of drug release,
and kinetic models of drug release of the prepared POQCL formulation.

Methods:
POQCL was prepared by emulsification-evaporation technique with some modifications
and evaluation was done by average particle size, surface morphological analysis, drug entrapment
percentage, drug loading, in vitro study of drug release, and kinetic models of drug release.

Results:
The average size of particle and surface morphology of prepared POQCL were found to be
76.89 nm and spherical in shape. The percentage yield was found to be 62.5% for the POQCL formulation. The percentages of drug entrapment efficiency and loading capacity were found to be 90%
and 47.36% respectively. The drug in vitro release outcomes were 24.27% within the 2 hours and
75.18% within 12 hours and followed the zero-order drug release kinetic model for the POQCL formulation.

Conclusion:
In this research study, we found that pilu oil is a useful novel lipid source in the formulation of liposome drug delivery for the encapsulation of BCS class IV drugs. POQCL formulation
showed optimum average particle size with enhanced entrapment efficiency and drug loading as well
as a sustained release of drug was found. In the future, the prepared liposomes of pilu oil may be
considered as the choice of drug delivery system for BCS class IV drugs.
Performance Differences of a Touch-Based Serial Reaction Time Task in Healthy Older Participants and Older Participants With Cognitive Impairment on a Tablet: Experimental Study
Authors: Mychajliw Christian , Holz Heiko , Minuth Nathalie , Dawidowsky Kristina , Eschweiler Gerhard Wilhelm , Metzger Florian Gerhard , Wortha Franz 
Published: December 2023

Background
Digital neuropsychological tools for diagnosing neurodegenerative diseases in the older population are becoming more relevant and widely adopted because of their diagnostic capabilities. In this context, explicit memory is mainly examined. The assessment of implicit memory occurs to a lesser extent. A common measure for this assessment is the serial reaction time task (SRTT).

Objective
This study aims to develop and empirically test a digital tablet–based SRTT in older participants with cognitive impairment (CoI) and healthy control (HC) participants. On the basis of the parameters of response accuracy, reaction time, and learning curve, we measure implicit learning and compare the HC and CoI groups.

Methods
A total of 45 individuals (n=27, 60% HCs and n=18, 40% participants with CoI—diagnosed by an interdisciplinary team) completed a tablet-based SRTT. They were presented with 4 blocks of stimuli in sequence and a fifth block that consisted of stimuli appearing in random order. Statistical and machine learning modeling approaches were used to investigate how healthy individuals and individuals with CoI differed in their task performance and implicit learning.

Results
Linear mixed-effects models showed that individuals with CoI had significantly higher error rates (b=−3.64, SE 0.86; z=−4.25; P<.001); higher reaction times (F1,41=22.32; P<.001); and lower implicit learning, measured via the response increase between sequence blocks and the random block (β=−0.34; SE 0.12; t=−2.81; P=.007). Furthermore, machine learning models based on these findings were able to reliably and accurately predict whether an individual was in the HC or CoI group, with an average prediction accuracy of 77.13% (95% CI 74.67%-81.33%).

Conclusions
Our results showed that the HC and CoI groups differed substantially in their performance in the SRTT. This highlights the promising potential of implicit learning paradigms in the detection of CoI. The short testing paradigm based on these results is easy to use in clinical practice.
DETERMINAN KINERJA INDUSTRI TPT INDONESIA
Authors: Nada Haya Marshella Lifnatin , Hermawan Iwan , Rambe Khoiru Rizqy , Nugraheni Reninta Dewi , Zuhdi Fadhlan , Isnasari Yovita , Asshagab Sri Milawati 
Published: December 2023

The textile and textile product (TPT) industry is a leading industry for national economic growth. The existence of its performance relies on various factors that turn out to be vulnerable to fluctuations, including the fulfillment of raw materials in the form of cotton fiber from import dominance. Therefore, disruption of the performance of the textile industry will also stimulate disruption to Indonesia's economic growth. This study aims to analyze the determinants of the development of textile industry performance. For this reason, a quantitative approach is used with econometric models prepared based on the Cobb-Douglas theory of production functions. The model construction uses secondary time series data for the period 1992-2021 sourced from the World Bank, Bank Indonesia, the International Monetary Fund (IMF), and the Central Statistics Agency (BPS). The findings show that the terminology of the development of the performance of the Indonesian textile industry includes capital, labor absorption, world cotton fiber prices, inflation, textile exports, the Covid-19 pandemic, and the global food crisis in 2007/2008. Therefore, several policy recommendations were submitted to encourage more massive TPT machine restructuring programs, improve the skills of TPT human resources (HR), provide incentives for Local Ease of Export Destinations (KLTE), and re-intensify the #banggabuatanindonesia campaign.
Visböcker i kulturella gränsland
Authors: Joons Gylling Sofia 
Published: December 2023

I denna artikel lyfter jag frågan vilken inverkan handskrivna visböcker har på formandet av en kulturell identitet. Forskningsmaterialet består av 28 visböcker skapade av 22 unga estlandssvenskar under perioden 1861-1945. Visböckerna innehåller både världsliga visor och andliga sånger mestadels på svenska och estniska. Jag har närläst visböckerna och genomfört intervjuer med personer som vet något om hur visböckerna skapats.
Teoretiskt placerar jag visböckerna på två musikaliska stigar (Finnegan 1989), den världsliga visstigen och den andliga sångstigen. Ingen av visorna i böckerna har författats och primärt spridits inom den estlandssvenska befolkningsgruppen. Därför placerar jag skapandet av visböcker i ett kulturellt gränsland, där individer har möjligheten att bekanta sig med en främmande kultur och se den egna kulturen från distans (Clifford 1992). Eftersom musikaliska stigar utgörs av situationer där individer möts uppfattar jag dem som upplevda gemenskaper där olika föreställda gemenskaper (Anderson 2016) aktualiseras genom visorna.
Min forskning visar hur visboksskaparna nått fram till kulturella gränsland genom att följa de två olika vis-stigarna. Visböckerna som skapats på den andliga sångstigen innehåller huvudsakligen sångtexter på svenska. Detta indikerar att den upplevda gemenskapen runt bönhusen i väckelsemiljöer skapat broar till föreställda kristna, bildade pansvenska gemenskaper genom sångerna.
De flesta världsliga visor på svenska har inte ombildats under införandet i visböcker. Därför uppfattar jag även dessa visböcker som uttryck för en pansvensk gemenskap. Ett par världsliga visor har medvetet ombildats under överföringen så de uttrycker en föreställd hembygdsgemenskap istället för den pansvenska.
Före 1918 utgjorde den estlandssvenska befolkningsgruppen en kulturell minoritet i det ryska imperiet och efter 1918 en kulturell minoritet i republiken Estland. Under mellankrigstiden infördes även visor på estniska i visböckerna. Eftersom de flesta visorna är relativt exakta kopior av populära sånger som spreds via inspelningar och tryckta sångtexter uppfattar jag dem som uttryck för kulturellt samgående (Slobin & Ronström 1988).

Artikelns temabild: Visböcker från Estlands svenskbygder. Foto: Sofia Joons Gylling.
Persona non grata vai arvostettu Sibelius-sopraano
Authors: Talasniemi Johanna 
Published: December 2023

Artikkelissa tarkastellaan laulajan uran toteutumista toisen maailmansodan jälkeisinä vuosina. Raskaiden sotavuosien jälkeen rauhaan asettauduttiin muuttuneessa poliittisessa ja yhteiskunnallisessa tilanteessa, mikä vaikutti myös muusikoiden mahdollisuuksiin. Sopraano Aulikki Rautawaara (1906–1990) on kiinnostava esimerkki laulajasta, jonka ura sodan jälkeen ei toteutunut sotaa edeltäneen uran viitoittamalla tiellä. Rautawaara työskenteli vuonna 1927 alkaneen laulajanuransa jälkipuolella etupäässä konserttilaulajana ja oli tunnettu erityisesti ja erityisenä Sibeliuksen laulujen esittäjänä.
Reseptiohistorian työkalujen ja elämäkertatutkimuksen lähestymistapojen avulla käsitellään, millaisen vastaanoton Rautawaara sai esiintyvänä taiteilijana toisen maailmansodan jälkimainingeissa. Selventävä kysymys on, miten Rautawaaran sodan aikainen toiminta vaikutti uraan sodan jälkeen. Lisäksi tarkastellaan, miten Rautawaara itse suhtautui tilanteeseen. Pääasiallisena aineistona ovat suomalaiset, ruotsalaiset ja norjalaiset sanomalehdet. Tätä aineistoa täydennetään muulla arkistoaineistolla esimerkiksi Aulikki Rautawaaran yksityisarkistosta.
Rautawaaran esiintymiset saksalaisille miehittäjäsotilaille Norjassa sodan aikana nousivat lehdistössä esiin aika ajoin sodan jälkeen ja vaikuttivat uran toteutumiseen. Musiikkiyhteisön tuki Suomessa ja Ruotsissa auttoi Rautawaaraa palaamaan 1940-luvun lopulla myös kansainvälisille estradeille. Rautawaaran tapaus on osa sopeutumista ja siinä kilvoittelua toisen maailmansodan jälkeisten vuosien muuttuneissa olosuhteissa.
Aulikki Rautawaara, laulajan ura, jälleenrakennus, Sibelius, kulttuurihistoria, laulajatutkimus

Artikkelin kansikuva: Aulikki Rautawaara. Lähde: Aulikki Rautawaaran yksityisarkisto.
Instances of Preaching in a Medieval Transylvanian Town. The Case of Cluj in the Century before the Reformation
Authors: ŞTEFAN Paula 
Published: December 2023

Preaching was an essential component of the religious life during the Middle Ages, shaping the Christian identity and promoting the norms and decisions of the Church. In line with recent contributions that have drawn attention to Transylvanian expressions of medieval preaching, the present paper intends to examine its significance in the urban milieu prior to the Protestant Reformation, by exploring the case of Cluj (hu. Kolozsvár/ germ. Klausenburg). An important town of the region, it was inhabited by a mixed population of Hungarian- and German-speaking communities, whose pastoral care and access to the divine Word was facilitated by various religious institutions. Although the extant sources do not provide detailed accounts regarding specific sermons delivered in town, their actual contents and mise-en-scène, the present paper outlines the profile of the routine preaching activity, based on homiletic works that belonged to the secular and regular clergy of the town, as well as charters attesting their complementary or competing actions. The analysis reveals that homiletic concerns progressively increased from the beginning of the 15th century onwards, reaching a pinnacle in the decades prior to the adoption of the Reformation.
Rezumat: Predica a constituit o componentă esențială a vieții religioase pe parcursul Evului Mediu, contribuind la fasonarea identității creştine şi la diseminarea normelor şi deciziilor Bisericii. Pe urma unor contribuții istoriografice recente care au atras atenția asupra expresiilor transilvănene ale acestui fenomen medieval, prezentul studiu propune examinarea importanței predicării în ambianță urbană, înainte de Reformă, prin sondarea cazului oraşului Cluj (magh. Kolozsvár/ germ. Klausenburg). Unul dintre centrele urbane majore ale regiunii, Clujul se distingea printr-o populație mixtă, de saşi şi maghiari, a căror pastorație intra în grija mai multor instituții religioase. Cu toate că sursele păstrate nu dezvăluie detalii despre cuvântările omiletice rostite în oraş, despre conținutul sau punerea lor în scenă, lucrarea schițează profilul activității uzuale de predicare pe baza colecțiilor de predici care au aparținut clericilor seculari şi fraților cerşetori, precum şi a surselor documentare care atestă actiunile complementare sau concurente ale acestora. Analiza dezvăluie că preocupările pastorale şi omiletice au cunoscut o creştere progresivă în decursul secolului al XV-lea, atingând apogeul in deceniile imediat anterioare adoptării Reformei.
Cuvinte cheie: predica medievală, istorie urbană, predicatori, ordine mendicante, cler parochial, pluralitate lingvistică
Article: history; Received: 16.05.2023; Revised: 23.05.2023; Accepted: 10.06.2023; Available online: 30.06.2023.