bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optogenetic Regulation of EphA1 RTK Activation and Signaling
Authors: Wurz Anna I. , Zheng Kevin S. , Hughes Robert M. 
Published: February 2024

AbstractEph receptors are ubiquitous class of transmembrane receptors that mediate cell-cell communication, proliferation, differentiation, and migration. EphA1 receptors specifically play an important role in angiogenesis, fetal development, and cancer progression; however, studies of this receptor can be challenging as its ligand, ephrinA1, binds and activates several EphA receptors simultaneously. Optogenetic strategies could be applied to circumvent this requirement for ligand activation and enable selective activation of the EphA1 subtype. In this work, we designed and tested several iterations of an optogenetic EphA1 - Cryptochrome 2 (Cry2) fusion, investigating their capacity to mimic EphA1-dependent signaling in response to light activation. We then characterized the key cell signaling target of MAPK phosphorylation activated in response to light stimulation. The optogenetic regulation of Eph receptor RTK signaling without the need for external stimulus promises to be an effective means of controlling individual Eph receptor-mediated activities and creates a path forward for the identification of new Eph-dependent functions.
Diversity, Origin and Evolution of the ESCRT Systems
Authors: Makarova Kira S. , Tobiasson Victor , Wolf Yuri I. , Lu Zhongyi , Liu Yang , Zhang Siyu , Krupovic Mart , Li Meng , Koonin Eugene V 
Published: February 2024

AbstractEndosomal Sorting Complexes Required for Transport (ESCRT) play key roles in protein sorting between membrane-bounded compartments of eukaryotic cells. Homologs of many ESCRT components are identifiable in various groups of archaea, especially in Asgardarchaeota, the archaeal phylum that is currently considered to include the closest relatives of eukaryotes, but not in bacteria. We performed a comprehensive search for ESCRT protein homologs in archaea and reconstructed ESCRT evolution using the phylogenetic tree of Vps4 ATPase (ESCRT IV) as a scaffold, using sensitive protein sequence analysis and comparison of structural models to identify previously unknown ESCRT proteins. Several distinct groups of ESCRT systems in archaea outside of Asgard were identified, including proteins structurally similar to ESCRT-I and ESCRT-II, and several other domains involved in protein sorting in eukaryotes, suggesting an early origin of these components. Additionally, distant homologs of CdvA proteins were identified in Thermoproteales which are likely components of the uncharacterized cell division system in these archaea. We propose an evolutionary scenario for the origin of eukaryotic and Asgard ESCRT complexes from ancestral building blocks, namely, the Vps4 ATPase, ESCRT-III components, wH (winged helix-turn-helix fold) and possibly also coiled-coil, and Vps28-like domains. The Last Archaeal Common Ancestor likely encompassed a complex ESCRT system that was involved in protein sorting. Subsequent evolution involved either simplification, as in the TACK superphylum, where ESCRT was co-opted for cell division, or complexification as in Asgardarchaeota. In Asgardarchaeota, the connection between ESCRT and the ubiquitin system that was previously considered a eukaryotic signature was already established.ImportanceAll eukaryotic cells possess complex intracellular membrane organization. ESCRT (Endosomal Sorting Complexes Required for Transport) plays a central role in membrane remodeling which is essential for cellular functionality in eukaryotes. Recently, it has been shown that Asgard archaea, the archaeal phylum that includes the closest known relatives of eukaryotes, encode homologs of many components of the ESCRT systems. We employed protein sequence and structure comparisons to reconstruct the evolution of ESCRT systems in archaea and identified several previously unknown homologs of ESCRT subunits, some of which can be predicted to participate in cell division. The results of this reconstruction indicate that the Last Archaeal Common ancestor already encoded a complex ESCRT system that was involved in protein sorting. In Asgard archaea, ESCRT systems evolved towards greater complexity, and in particular, the connection between ESCRT and the ubiquitin system that was previously considered a eukaryotic signature was established.
Standard rodent diets differentially impact alcohol consumption and preference and gut microbiome diversity
Authors: Zaparte Aline , Dore Evan , White Selby , Paliarin Franciely , Gabriel Cameron , Copenhaver Katherine , Basavanhalli Samhita , Garcia Emily , Vaddavalli Rishith , Luo Meng , Taylor Christopher M. , Welsh David , Maiya Rajani 
Published: February 2024

AbstractAlcohol Use Disorder (AUD) is a complex and widespread disease with limited pharmacotherapies. Preclinical animal models of AUD use a variety of voluntary alcohol consumption procedures to recapitulate different phases of AUD including binge alcohol consumption and dependence. However, voluntary alcohol consumption in mice is widely variable rendering it difficult to reproduce results across labs. Accumulating evidence indicates that different brands of commercially available rodent chow can profoundly influence alcohol intake. In this study, we investigated the effects of three commercially available and widely used rodent diet formulations on alcohol consumption and preference in C57BL/6J mice using the 24h intermittent access procedure. The three brands of chow tested were LabDiet 5001 (LD 5001), LabDiet 5053 (LD 5053), and Teklad 2019S (TL2019S) from two companies (Research Diets and Envigo respectively). Mice fed LD5001 displayed the highest levels of alcohol consumption and preference followed by LD5053 and TL2019S. We also found that alcohol consumption and preference could be rapidly switched by changing the diet 48h prior to alcohol administration. Sucrose, saccharin, and quinine preference were not altered suggesting that the diets did not alter taste perception. We also found that mice fed LD5001 displayed increased quinine-resistant alcohol intake compared to mice fed TL2019S, suggesting that diets could influence the development of “compulsive” like alcohol consumption. We profiled the gut microbiome of water and alcohol drinking mice that were maintained on different diets and found significant differences in bacterial alpha and beta diversity, which could impact gut-brain axis signaling and alcohol consumption.
The association of lifestyle with cardiovascular and all-cause mortality based on machine learning: A Prospective Study from the NHANES
Authors: Guo Xinghong , Wu Jian , Ma Mingze , Tarimo Clifford Silver , Fei Fengyi , Zhao Lipei , Ye Beizhu 
Published: February 2024

AbstractObjectiveTo develop a machine learning (ML) risk stratification model for predicting all-cause mortality and cardiovascular mortality while estimating the influence of lifestyle behavioral factors on the model’s efficacy.MethodA prospective cohort study was conducted using a nationally representative sample of adults aged 40 years or older, drawn from the US National Health and Nutrition Examination Survey from 2007 to 2010. The participants underwent a comprehensive in-person interview and medical laboratory examinations, and subsequently, their records were linked with the National Death Index for further analysis.ResultWithin a cohort comprising 7921 participants, spanning an average follow-up duration of 9.75 years, a total of 1911 deaths, including 585 cardiovascular-related deaths, were recorded. The model predicted mortality with an area under the receiver operating characteristic curve (AUC) of 0.848 and 0.829. Stratifying participants into distinct risk groups based on ML scores proved effective. All lifestyle behaviors exhibited an inverse association with all-cause and cardiovascular mortality. As age increases, the discernible impacts of dietary scores and sedentary time become increasingly apparent, whereas an opposite trend was observed for physical activity.ConclusionWe develop a ML model based on lifestyle behaviors to predict all-cause and cardiovascular mortality. The developed model offers valuable insights for the assessment of individual lifestyle-related risks. It applies to individuals, healthcare professionals, and policymakers to make informed decisions.
Effects of intervention stage completion in an integrated behavioral health and primary care randomized pragmatic intervention trial
Authors: Stephens Kari A. , van Eeghen Constance , Zheng Zihan , Anastas Tracy , Ma Kris Pui Kwan , Prado Maria G. , Clifton Jessica , Rose Gail , Mullin Daniel , Chan Kwun C. G. , Kessler Rodger 
Published: February 2024

AbstractPurposeA pragmatic, cluster-randomized controlled trial of a comprehensive practice-level, multi-staged practice transformation intervention aimed to increase behavioral health integration in primary care practices and improve patient outcomes. We examined association between the completion of intervention stages and patient outcomes across a heterogenous national sample of primary care practices.MethodsForty-two primary care practices across the U.S. with co-located behavioral health and 2,426 patients with multiple chronic medical and behavioral health conditions completed surveys at baseline, midpoint and two year follow-up. Effects of the intervention on patient health and primary care integration outcomes were examined using multilevel mixed-effects models, while controlling for baseline outcome measurements.ResultsNo differences were found associated with the number of intervention stages completed in patient health outcomes were found for depression, anxiety, fatigue, sleep disturbance, pain, pain interference, social function, patient satisfaction with care or medication adherence. The completion of each intervention stage was associated with increases in Practice Integration Profile (PIP) domain scores and were confirmed with modeling using multiple imputation for: Workflow 3.5 (95% CI: 0.9-6.1), Integration Methods 4.6 (95% CI: 1.5-7.6), Patient Identification 2.9 (95% CI: 0.9-5.0), and Total Integration 2.7 (95% CI: 0.7-4.7).ConclusionA practice-centric flexible practice transformation intervention improved integration of behavioral health in primary care across heterogenous primary care practices treating patients with multiple chronic conditions. Interventions that allow practices to flexibly improve care have potential to help complex patient populations. Future research is needed to determine how to best target patient health outcomes at a population level.
Neurotensin-Neurotensin Receptor 2 signaling in adipocytes regulates food intake through ceramide metabolism
Authors: Fu Wei , Yang Yue , Guo Xiao , Gong Qifan , Zhou Xiaofeng , Zhou Liying , Liu Cenxi , Zhang Zhi , So Jisun , Zhang Yufeng , Huang Lin , Lu Guangxing , Yi Chuanyou , Wang Qichu , Fan Chenyu , Liu Chao , Wang Jiaxing , Yu Haiyi , Zhao Yimin , Huang Tao , Roh Hyun Cheol , Liu Tiemin , Tang Huiru , Qi Jianping , Xu Ming , Zheng Yan , Huang He , Li Jin 
Published: February 2024

SUMMARYNeurotensin (NTS) is a secretory peptide produced by the lymphatic endothelial cells (LEC). Our previous study revealed that NTS suppressed the activity of brown adipose tissue via the interactions with NTSR2. In the current study, we found that the depletion ofNtsr2in the white adipocytes upregulated food intake, while the local treatment of NTS suppressed the food intake. Mechanistic study revealed that the suppression of NTS-NTSR2 signaling enhanced the phosphorylation of ceramide synthetase 2 (CerS2), increased the abundance of its products ceramide C20-C24 and downregulated the production of GDF15 in the white adipose tissues, which was responsible for the elevation of food intake. With four populations of different age and ethnic background, we discovered a potential causal and positive correlation between ceramide C20-24 and food intake in human. Our study identified that NTS-NTSR2 signaling can perform the neurological regulation via controlling the production of ceramide in the white adipocytes.
Telephone training to improve ECG quality in remote screening for atrial fibrillation
Authors: Bhayankaram Kethaki Prathivadi , Mant Jonathan , Brimicombe James , Dymond Andrew , Williams Kate , Charlton Peter H. 
Published: February 2024

AbstractBackground and AimsSelf-recorded, single-lead ECGs are increasingly used to diagnose arrhythmias. However, they can be of variable quality, which can affect the reliability of interpretation. In this analysis of ECGs collected in atrial fibrillation screening studies, our aims were to: (i) determine the quality of ECGs when recorded unsupervised (at home); and (ii) investigate whether telephone training improved ECG quality.MethodsData was obtained from the Screening for Atrial Fibrillation to Reduce stroke (SAFER) programme, where participants recorded single-lead ECGs four times per day for three weeks using a handheld device. ECG quality was assessed using an automated algorithm, and participants who recorded >25% poor quality ECGs from days 4-10 of screening were identified for training. Telephone training to improve ECG recording technique was delivered when research team capacity permitted.Results14,727 participants recorded 1,206,972 ECGs, of which 43,513 (3.6%) were poor quality. Most participants (51.9%) did not record any poor-quality ECGs. 1,105 (7.5%) participants met the threshold for training. Of these, 165 participants received training and 896 did not. Comparing these groups, the mean (95% confidence interval) reduction in the proportion of poor-quality ECGs per participant from before training (days 1-3) to after training (days 11-21) was 21.1 (17.5-23.5) % with training and 15.7 (14.5-16.8) % without training (p<0.05).ConclusionMost participants achieved adequate quality ECGs. For those that did not, ECG quality improved over time regardless, and training further improved ECG quality. Therefore, telephone training could be considered in atrial fibrillation screening programmes using single-lead ECG devices.What’s new?- This is the first study to explore telephone training as a way to improve the quality of ECGs.- We found that the quality of ECGs recorded by participants increased naturally over time, regardless of whether they received telephone training. A minimum time period of screening is required to allow for this natural improvement in quality.- Telephone training was found to be beneficial in improving ECG quality, and was associated with greater improvement in quality than due to natural improvement alone.- Real-time transmission of ECGs and assessment of ECG quality may be useful to identify participants who may benefit from telephone training.- The quality of ECGs obtained at home is comparable when using an entirely remote process (postal device delivery and optional telephone training) vs. using an initial face-to-face training session (in-person device setup and training from GP practice nurses).
A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing
Authors: Lukhovitskaya Nina , Brown Katherine , Hua Lei , Pate Adrienne E. , Carr John P. , Firth Andrew E. 
Published: February 2024

ABSTRACTIlarviruses are a relatively understudied but important group of plant RNA viruses that includes a number of crop pathogens. Their genomes comprise three RNA segments encoding two replicase subunits, movement protein, coat protein (CP), and (in some ilarvirus subgroups) a protein that suppresses RNA silencing. Here we report that, in many ilarviruses, RNA3 encodes an additional protein (termed CP-RT) as a result of ribosomal readthrough of the CP stop codon into a short downstream readthrough (RT) ORF. Using asparagus virus 2 as a model, we find that CP-RT is expressedin plantawhere it functions as a weak suppressor of RNA silencing. CP-RT expression is essential for persistent systemic infection in leaves and shoot apical meristem. CP-RT function is dependent on a putative zinc-finger motif within RT. Replacing the asparagus virus 2 RT with the RT of an ilarvirus from a different subgroup restored the ability to establish persistent infection. These findings open up a new avenue for research on ilarvirus silencing suppression, persistent meristem invasion and vertical transmission.
Detecting High-Energy Neutrinos from Galactic Supernovae with ATLAS
Authors: Wen Alex Y. , Argüelles Carlos A. , Kheirandish Ali , Murase Kohta 
Published: February 2024

We show that ATLAS, a collider detector, can measure the flux of high-energy supernova neutrinos, which can be produced from days to months after the explosion. Using Monte Carlo simulations for predicted fluxes, we find at most O(0.1–1) starting events and O(10–100) throughgoing events from a supernova 10 kpc away. Possible Galactic supernovae from Betelgeuse and Eta Carinae are further analyzed as demonstrative examples. We argue that, even with limited statistics, ATLAS has the ability to discriminate among flavors and between neutrinos and antineutrinos, making it a unique neutrino observatory so far unmatched in this capability.

Published by the American Physical Society
2024
Efficient high-fidelity flying qubit shaping
Authors: Tissot Benedikt , Burkard Guido 
Published: February 2024

Matter qubit to traveling photonic qubit conversion is the cornerstone of numerous quantum technologies such as distributed quantum computing, as well as several quantum internet and networking protocols. We formulate a theory for stimulated Raman emission which is applicable to a wide range of physical systems, including quantum dots, solid-state defects, and trapped ions, as well as various parameter regimes. We find the upper bound for the photonic pulse emission efficiency of arbitrary matter qubit states for imperfect emitters and show a path forward to optimizing the fidelity. Based on these results, we propose a paradigm shift from optimizing the drive to directly optimizing the temporal mode of the flying qubit using a closed-form expression. Protocols for the production of time-bin encoding and spin-photon entanglement are proposed. Furthermore, the mathematical idea to use input-output theory for pulses to absorb the dominant emission process into the coherent dynamics, followed by a non-Hermitian Schrödinger equation approach, has great potential for studying other physical systems.

Published by the American Physical Society
2024
Sustainable and healthy food consumption in Europe: an analysis of consumer purchase patterns, motivations and barriers towards foods from SFSCs
Authors: Hyland John , Henchion Maeve Mary , Olomo Oluwayemisi , Attard Jennifer , Gaffey James 
Published: February 2024

PurposeThe aim of this paper is to better understand European consumers' behaviour in relation to Short Food Supply Chains (SFSCs), so as to provide insights to support their development as part of a sustainable food system. Specifically, it aims to analyse consumer purchase patterns, motivations and perceived barriers and to identify patterns of behaviour amongst different consumer groups.Design/methodology/approachAn online consumer survey was conducted in 12 European countries (n = 2,419). Quantitative data analysis, including principal component analysis (PCA) and cluster analysis, was undertaken using SPSS.FindingsFour consumer clusters are named according to their behavioural stage in terms of SFSC engagement: Unaware Unengaged, Aware Unengaged, Motivationally Engaged and Executively Engaged. Unaware Unengaged and Aware Unengaged are in the non-engagement phase of behaviour. Motivationally Engaged are motivationally activated to engage in the behaviour but fail to do so consistently. Executively Engaged is the fully engaged cluster, being motivated to act and purchasing local food on a frequent basis. The results show an interesting interplay between motivations and barriers, i.e. higher scores for motivations and lower scores for barriers do not necessarily translate into higher purchase frequency.Originality/valueThe research gleans insights into the contextual factors that may inhibit SFSC purchases in different consumer segments. It offers practical implications for policymakers and others seeking to develop SFSCs as part of a sustainable food system.
Stepping stones across a fast-flowing river: supporting emerging scholars from emerging markets
Authors: Barnard Helena 
Published: February 2024

Purpose
International business as a field values perspectives from various contexts, but scholars from emerging markets face a number of often-unseen challenges preventing them from fully contributing to the field. This study aims to explain those challenges and what the author has done to manage them.

Design/methodology/approach
This is a Reflexive piece in which the author makes sense of her own experience as a scholar not only of but also in an emerging market, as well as the experiences at her school in seeking to develop a high-quality doctoral program.

Findings
When leading scholars interact, whether in writing or in person, they tend to be in academically and otherwise well-resourced locations. This is true even for the leading international business scholars of emerging markets, and it imposes time and financial costs on scholars located in emerging markets wanting to participate in such conversations. Having experienced such challenges, the author worked with colleagues to design a doctoral program that could nurture rich scholarly conversations at the school. However, there remains a clear and pervasive tension between the inclusive aspirations of the field and the tendency for cutting-edge academic conversations to be concentrated in the developed world.

Originality/value
The experiences of emerging market scholars remain underrepresented in the field. By drawing on her own experience, both as a scholar and the head of the doctoral program at her school, the author provides suggestions for how to think about and develop a more inclusive scholarly conversation.