bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of hydroxyl containing residues in the intracellular region of rat bradykinin B2 receptor in signal transduction, receptor internalization, and resensitization
Authors: Prado Gregory N. , Mierke Dale F. , LeBlanc Thomas , Manseau Marc , Taylor Linda , Yu Jun , Zhang Ran , Pal‐Ghosh Ruma , Polgar Peter 
Published: August 2002

AbstractIn past reports we illustrated the importance of Y131, Y322, and T137 within the intracellular (IC) face of the rat bradykinin B2 receptor (rBKB2R) for signal transduction and receptor maintenance (Prado et al. [1997] J. Biol. Chem. 272:14638–14642; Prado et al. [1998] J. Biol. Chem. 273:33548–33555). In this report, we mutate the remaining hydroxyl possessing residues located within the rBKB2R IC region. Exchange of S139A (IC2) or T239V (IC3) did not affect BK activated phosphatidylinositol (PI) turnover or receptor internalization. Chimeric exchange of the last 34 amino acids of BKB2R C‐terminus with the corresponding 34 amino acids of the rat angiotensin II AT1a receptor (rAT1aR), both containing an S/T cluster, resulted in a mutant with normal endocytosis and BK activated PI turnover. A more selective chimera of these S/T clusters, with an exchange of BKB2R (333–351) with a rAT1aR fragment (326–342), resulted in a receptor with a retarded internalization but a normal BK activated PI turnover. Subsequent mutation of rBKB2R T344V showed little change in receptor uptake but a pronounced loss of BK activated PI turnover. The mutation of S335A, S341A, S348A, and S350A resulted in very poor receptor internalization and loss of activated PI turnover. Closer examination of this serine cluster illustrated that the replacement of S348A led to poor internalization; whereas the retention of S348 and mutation of S341A resulted in a receptor with a much greater internalization than WT. These and other results suggest that the presence of S348 promotes internalization while the presence of S341 dampens it. Conversely, S341 and S350 proved important for receptor signaling. In sum, our results illustrate that the distal C‐terminus including its S/T cluster is important for both rBKB2R internalization and signal transduction. Individual S/T residues within this cluster appear involved in either signal transmission or receptor uptake capacity. However, replacement of the entire distal tail region with the corresponding rAT1aR sequence, also containing an S/T cluster, enables the BKB2R/AT1aR chimera to act in a very similar manner to wild type rBKB2R. J. Cell. Biochem. 83: 435–447, 2001. © 2001 Wiley‐Liss, Inc.
Suppression of cell proliferation and deoxyribonucleic acid synthesis in the cloned rat hepatoma H4‐II‐E cells overexpressing regucalcin
Authors: Misawa Hiroyuki , Inagaki Shyuichiroh , Yamaguchi Masayosi 
Published: August 2002

AbstractThe role of endogenous regucalcin (RC) in the regulation of cell proliferation was investigated in the cloned rat hepatoma H4‐II‐E cells overexpressing RC stably. H4‐II‐E cells were transfected with RC/pCXN2 vector and the multiple neomycin‐resistant clones which overexpress stably RC were selected. The RC content of RC/pCXN2‐transfected cells used in this study was 19.7‐fold as compared with that of the parental wild type H4‐II‐E cells. Wild type H4‐II‐E cells, pCXN2 vector‐transfected cells (mock type), and RC/pCXN2‐transfected cells (transfectants) were cultured for 24, 48, and 72 h in the presence of fetal bovine serum (10% FBS). Cell numbers of wild and mock type were significantly increased with the time course of culture. Cell numbers of transfectants was significantly suppressed as compared with that of wild and mock type. Deoxyribonucleic acid (DNA) synthesis activity in the nuclear fraction of H4‐II‐E cells was significantly suppressed in transfectants with culture for 12–48 h. The presence of anti‐RC monoclonal antibody (10–50 ng/ml) in the reaction mixture caused a significant increase in DNA synthesis activity in the nuclei of wild type and transfectants; this increase was remarkable in transfectants. The effect of anti‐RC monoclonal antibody (50 ng/ml) in increasing DNA synthesis activity in transfectants was completely prevented by the addition of regucalcin (1 μM). This study demonstrates that cell proliferation is suppressed in the cloned rat hepatoma H4‐II‐E overexpressing RC stably. J. Cell. Biochem. 84: 143–149, 2002. © 2001 Wiley‐Liss, Inc.
Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP120 binding studies
Authors: Kennedy Derek , French Juliet , Guitard Estelle , Ru Kelin , Tocque Bruno , Mattick John 
Published: August 2002

AbstractThe G3BP (ras‐GTPase‐Activating Protein SH3‐Domain‐Binding Protein) family of proteins has been implicated in both signal transduction and RNA‐metabolism. We have previously identified human G3BP‐1, G3BP‐2, and mouse G3BP‐2. Here, we report the cloning of mouse G3BP‐1, the discovery of two alternatively spliced isoforms of mouse, and human G3BP‐2 (G3BP‐2a and G3BP‐2b), and the chromosomal localisation of human G3BP‐1 and G3BP‐2, which map to 5q14.2‐5q33.3 and 4q12‐4q24 respectively. We mapped the rasGAP120 interactive region of the G3BP‐2 isoforms and show that both G3BP‐2a and G3BP‐2b use an N‐terminal NTF2‐like domain for rasGAP120 binding rather than several available proline‐rich (PxxP) motifs found in members of the G3BPs. Furthermore, we have characterized the protein expression of both G3BP‐1 and G3BP‐2a/b in adult mouse tissues, and show them to be both tissue and isoform specific. J. Cell. Biochem. 84: 173–187, 2002. © 2001 Wiley‐Liss, Inc.
Density functional theory for efficient ab initio molecular dynamics simulations in solution
Authors: Fattebert Jean‐Luc , Gygi François 
Published: August 2002

AbstractWe present a density functional for first‐principles molecular dynamics simulations that includes the electrostatic effects of a continuous dielectric medium. It allows for numerical simulations of molecules in solution in a model polar solvent. We propose a smooth dielectric model function to model solvation into water and demonstrate its good numerical properties for total energy calculations and constant energy molecular dynamics. © 2002 Wiley Periodicals, Inc. J Comput Chem 23: 662–666, 2002
NMR 3J(C1,H3) couplings in 1‐X‐bicyclo[1.1.1]pentanes. FPT–DFT and NBO studies of hyperconjugative interactions and heavy atom substituent effects
Authors: Barone Verónica , Peralta Juan E. , Contreras Rubén H. 
Published: August 2002

AbstractIn this work a rationalization of the very large substituent effects on 3J(C1,H3) couplings in 1‐X‐bicyclo[1.1.1]pentanes is presented. The Fermi contact contribution to such couplings was calculated in a series of 13 X‐derivatives within the DFT–B3LYP framework using the finite perturbation theory. Core electrons for atoms beyond the Second Row were taken into account using effective core potentials. Calculated couplings are in very good agreement with experimental values. The role played by hyperconjugative interactions involving bonds or antibonds belonging to the coupling pathway are studied using the NBO approach. Heavy atom contribution to substituent effects on 3J(C1,H3) couplings was estimated as small. This contrasts notably with trends observed in the corresponding 13C substituent chemical shifts, SCSs. The latter were estimated comparing for X=Cl, Br, I, SnMe3, calculated SCSs with their experimental values. Such estimations are in line with explicit calculations of the spin‐orbit contribution reported in the literature for smaller compounds. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 1615–1621, 2001
On the state of strength‐three covering arrays
Authors: Chateauneuf M. , Kreher D. L. 
Published: August 2002

AbstractA covering array of size N, strength t, degree k, and order υ is a k × N array on υ symbols in which every t × N subarray contains every possible t × 1 column at least once. We present explicit constructions, constructive upper bounds on the size of various covering arrays, and compare our results with those of a commercial product. Applications of covering arrays include software testing, drug screening, and data compression. © 2002 Wiley Periodicals, Inc. J Combin Designs 10: 217–238, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jcd.10002
Analysis of 3‐hydroxydodecanedioic acid for studies of fatty acid metabolic disorders: Preparation of stable isotope standards
Authors: Chickos J.S. , Way Barbara A. , Wilson J. , Shaharuzzaman M. , Laird J. , Landt M. 
Published: August 2002

AbstractCurrent diagnostic tests to detect disorders of fatty acids metabolism, such as long‐chain hydroxyacyl CoA dehydrogenase deficiency (LCHAD), are hampered by insensitivity or a long delay time required for results. Children with LCHAD deficiency are known to excrete 3‐hydroxydicarboxylic acids with chain lengths of 10–16 carbons, but a quantitative method to measure excretion of these potentially diagnostically important compounds has not been reported. We report synthetic schemes for synthesis of 3‐hydroxydodecanedioic acid and a di‐deuterated analog, suitable for use in a stable‐isotope dilution mass spectrometric analytical approach. Evaluation of several common derivatization protocols to produce a volatile derivative for gas chromatography determined that trimethylsyl derivatives produced the best efficiency and stability. Positive‐ion chemical ionization mass spectrometry provided the greatest yield of characteristic ions. These results indicate the basic reagents needed to develop sensitive and accurate 3‐hydroxydodecanedioic acid measurements for diagnosis of LCHAD deficiency and other fatty acid oxidation disorders. J. Clin. Lab. Anal. 16:115–120, 2002. © 2002 Wiley‐Liss, Inc.
Rapid in vitro conversion of fosphenytoin into phenytoin in sera of patients with liver disease: Role of alkaline phosphatase
Authors: Dasgupta Amitava , Schlette Ellen 
Published: August 2002

AbstractFosphenytoin, a phosphate ester pro drug of phenytoin, also cross‐reacts with the fluorescence polarization immunoassay (FPIA) for phenytoin. We measured fosphenytoin concentrations using the FPIA kit and TDx analyzer. We prepared serum pools from normal volunteers and patients with liver disease. None of them received either fosphenytoin or phenytoin. Fosphenytoin standard solution (1 mg/ml) was prepared in water. We supplemented aliquots of normal and liver pools with known amounts of fosphenytoin and measured the concentrations at different time intervals. The conversion of fosphenytoin to phenytoin was slow in sera with normal alkaline phosphatase activities. The conversion was rapid in sera collected from patients with liver disease who also had high alkaline phosphatase activities. The observed concentrations were close to target concentrations within 0–2 min of supplementation with fosphenytoin. Surprisingly, the observed concentration then started to decline slightly but significantly with longer incubation time. In contrast, the observed concentration increased steadily in serum with normal alkaline phosphatase activity. For example, in the normal pool supplemented with 15.0 μg/ml fosphenytoin (as the phenytoin equivalent), the observed concentrations were 6.9, 7.3, 7.7, 8.3, and 9.8 μg/ml at 0–2, 10, 20, 30, and 60 min, respectively. However, in a serum pool prepared from patients with liver disease and supplemented with 15.0 μg/ml of fosphenytoin (alkaline phosphatase: 2547 U/l), the observed phenytoin concentrations were 12.9, 12.1, 11.0, 10.7, and 10.7 μg/ml at 0–2, 10, 20, 30, and 60 min, respectively. When we added alkaline phosphatase to the normal serum pool, we observed rapid conversion of fosphenytoin into phenytoin within 10 min, but the concentrations then declined with longer incubation time. However, when we repeated the experiment with protein‐free ultrafiltrate, we observed rapid conversion of fosphenytoin to phenytoin, but the concentration did not decline with longer incubation time. J. Clin. Lab. Anal. 15:244–250, 2001. © 2001 Wiley‐Liss, Inc.
Urinary levels of monocyte chemoattractant protein‐1 (MCP‐1) and interleukin‐8 (IL‐8), and renal injuries in patients with type 2 diabetic nephropathy
Authors: Tashiro Kyouichi , Koyanagi Ichiro , Saitoh Akemi , Shimizu Ayumi , Shike Toshihide , Ishiguro Chizuru , Koizumi Michiko , Funabiki Kazuhiko , Horikoshi Satoshi , Shirato Isao , Tomino Yasuhiko 
Published: August 2002

AbstractWe examined the correlation among the levels of urinary monocyte chemoattractant protein‐1 (MCP‐1) and interleukin‐8 (IL‐8), hyperglycemia, and renal injuries in patients with type 2 diabetic nephropathy. The levels of urinary MCP‐1, IL‐8, protein excretion, blood urea nitrogen (BUN), serum creatinine (s‐Cr), glycohemoglobin A1c (HbA1c), and fasting plasma glucose (FPG) were measured in 24 patients with type 2 diabetic nephropathy and 14 healthy adults as controls. Diabetic nephropathy was classified into three stages: stage 1 = normoalbuminuric, stage 2 = microalbuminuric, and stage 3 = macroalbuminuric. All of the patients showed normal ranges in renal function tests. Levels of urinary MCP‐1 in all patients with diabetic nephropathy were significantly higher than those in healthy adults (P < 0.05). The levels of urinary MCP‐1 in patients with diabetic nephropathy increased gradually according to the clinical stage of this disease. In contrast, the levels of urinary IL‐8 in patients with diabetic nephropathy increased in stages 2 and 3. There was a significant correlation between the levels of urinary IL‐8 and those of HbA1c. High glucose may stimulate MCP‐1 and/or IL‐8 production and their excretion into the urine independently of the phases or pathological lesions of this disease. It appears that IL‐8 increased in the early stage of diabetic nephropathy, and MCP‐1 increased in the advanced stage of this disease. It was concluded that measurement of urinary MCP‐1 and IL‐8 may be useful for evaluating the degree of renal injuries in patients with type 2 diabetic nephropathy. J. Clin. Lab. Anal. 16:1–4, 2002. © 2002 Wiley‐Liss, Inc.
Responding to society's needs: Prescription privileges for psychologists
Authors: Norfleet Mary Ann 
Published: August 2002

AbstractThe health care revolution has contributed to the natural evolution of the role of psychologists. This has led to the necessity for future psychologists to have the authority to prescribe psychotropic medications in order to offer the best‐available, comprehensive treatment to the public. Psychologists' training gives them a unique role in addressing the psychosocial aspects of medical problems, in collaboration with primary‐care physicians. Prescribing psychologists are cost‐effective, many practice in rural areas where people have no other access to mental health care, and they will be able to treat other underserved populations such as the poor, the elderly, the chronically mentally ill, children, and prisoners in the criminal justice system. Prescribing psychologists will have an increasingly prominent role in future health care policy decisions and practice. © 2002 Wiley Periodicals, Inc. J Clin Psychol 58: 599–610, 2002.
Irrational beliefs, attitudes about competition, and splitting
Authors: Watson P.J. , Morris Ronald J. , Miller Liv 
Published: August 2002

AbstractRational–Emotive Behavior Therapy (REBT) theoretically promotes actualization of both individualistic and social‐oriented potentials. In a test of this assumption, the Belief Scale and subscales from the Survey of Personal Beliefs served as measures of what REBT presumes to be pathogenic irrationalities. These measures were correlated with the Hypercompetitive Attitude Scale (HCAS), the Personal Development Competitive Attitude Scale (PDCAS), factors from the Splitting Index, and self‐esteem. Results for the HCAS and Self‐Splitting supported the REBT claim about individualistic self‐actualization. Mostly nonsignificant and a few counterintuitive linkages were observed for irrational beliefs with the PDCAS, Family‐Splitting, and Other‐Splitting, and these data suggested that REBT may be less successful in capturing the “rationality” of a social‐oriented self‐actualization. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 343–354, 2001.
Psychological issues and treatments for people with diabetes
Authors: Rubin Richard R. , Peyrot Mark 
Published: August 2002

AbstractThis article examines psychological issues and their treatment among people with diabetes. The paper contains two main sections, one dealing with diagnosable clinical disorders, and the other with more mundane but nevertheless important subclinical problems in living with diabetes. We review the published literature on prevalence, manifestation, consequences, and treatment of psychological disorders in persons with diabetes, primarily depression, anxiety, and eating disorders. In describing everyday problems in living with diabetes we expand our sources beyond the published literature to include our own clinical and consulting experiences as well as our unpublished qualitative research. These problems include dietary restrictions, self‐monitoring of blood glucose, taking insulin injections, and lack of support from family and health care professionals. We describe methods for dealing with such problems and discuss the tension between focusing on emotional distress versus practical issues of disease management. Finally, we briefly present some potentially positive consequences of living with diabetes so that readers can be aware of the inspirational aspects of personal experience with this disease. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 457–478, 2001
Syzygy, science, and psychotherapy: The Consumer Reports study
Authors: Howard Kenneth I. , Krause Merton S. , Caburnay Charlene A. , Noel Susan B. , Saunders Stephen M. 
Published: August 2002

AbstractResults from a Consumer Reports (CR) survey indicated that psychotherapy has proven to be quite effective and that longer‐term therapy has been more effective than shorter‐term therapy. Critiques of the methodology of this study have included the claim that (a) the self‐selected sample was biased in favor of people who felt that they had benefited from psychotherapy, (b) the use of retrospective accounts led to a further positive bias, and (c) the validity of the outcome assessment was questionable. Supplemental data from other sources, including prospective data from a large sample of psychotherapy patients, are presented to augment the interpretation of the results of the CR study and to illustrate how some critiques of research results can be evaluated systematically. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 865–874, 2001.
Is EMDR an exposure therapy? A review of trauma protocols
Authors: Rogers Susan , Silver Steven M. 
Published: August 2002

AbstractThis article presents the well established theoretical base and clinical practice of exposure therapy for trauma. Necessary requirements for positive treatment results and contraindicated procedures are reviewed. EMDR is contrasted with these requirements and procedures. By the definitions and clinical practice of exposure therapy, the classification of EMDR poses some problems. As seen from the exposure therapy paradigm, its lack of physiological habituation and use of spontaneous association should result in negligible or negative effects rather than the well researched positive outcomes. Possible reasons for the effectiveness of EMDR are discussed, ranging from the fundamental nature of trauma reactions to the nonexposure mechanisms utilized in information processing models. © 2002 John Wiley & Sons, Inc. J Clin Psychol 58: 43–59, 2002.
A case illustration of resistance from a psychodynamic perspective
Authors: Satten Wayne 
Published: August 2002

AbstractThis clinical vignette illustrates a case of resistance during the course of a psychodynamic psychotherapy. The client is a chronically depressed woman struggling to expand beyond her familiar and, in many ways, gratifying role as a “good little girl” who seeks and often obtains reassurance and appreciation from paternalistic figures. She also has become increasingly aware of the cost of rigidly maintaining this role, which inhibits her experience of herself as a competent, assertive, and sexually mature woman. The client is at an advanced stage of her therapy, and in the vignette one sees her working through this conflict by addressing her experience both in her family of origin and in the therapeutic relationship. Her efforts to resolve this core issue, and her concurrent resistances, are exhibited in this presentation. © 2002 John Wiley & Sons, Inc. J Clin Psychol/In Session 58: 139–144, 2002.
Delivering the goods: The importance of screening accuracy for effective community intervention and prevention
Authors: Shenassa Edmond D. 
Published: August 2002

AbstractThe utility and feasibility of community intervention and prevention programs depend greatly on the accuracy by which programs' participants are screened from among community dwellers. This is because indiscriminate application of the program to the entire community benefits only a few, while it can even harm the many others. It is therefore necessary to define and screen a target population so the program can be delivered exclusively to them. Consequently, screening accuracy has implications for the plan, design, and evaluation of community‐based programs. In this manuscript indices of screening accuracy are discussed in the context of screening for substance use, and depression. Receiver Operating Characteristic curves, measurement error, as well as design and evaluation of community programs are also discussed in the context of screening accuracy. © 2002 John Wiley & Sons, Inc.
Myocilin is associated with mitochondria in human trabecular meshwork cells
Authors: Wentz‐Hunter Kelly , Ueda Jun , Shimizu Nibuyoshi , Yue Beatrice Y.J.T. 
Published: August 2002

AbstractThe trabecular meshwork (TM) is a specialized tissue located at the chamber angle of the eye next to the cornea. This tissue is believed to be responsible for regulation of the aqueous humor outflow and control of the intraocular pressure (IOP). Alterations in functions of the TM may lead to IOP elevation and development of glaucoma, a major cause of blindness. The myocilin gene has recently been directly linked to open‐angle glaucomas. The gene product was originally identified as a protein inducible in TM cells by treatment with glucocorticoids such as dexamethasone (DEX) and termed TIGR (TM inducible‐glucocorticoid response). The exact nature and function of the myocilin protein so far still remain elusive. In this study, myocilin was localized to the perinuclear region of both DEX‐treated and control TM cells. Its distribution overlapped considerably with that of mitochondria. Subcellular fractionation and Western blot analyses suggested a rather extensive association of myocilin with mitochondria. The DEX‐treated TM cells were found to undergo apoptosis, when exposed to anti‐Fas antibody, to a significantly higher degree than the untreated control cells. It appears that the TM cell integrity remains intact after DEX treatment. However, the induced myocilin or myocilin‐mitochondria association seems to render the cells more susceptible to a second stress or challenge. This vulnerability may be the basis that ultimately leads to pathological consequences. J. Cell. Physiol. 190: 46–53, 2002. © 2002 Wiley‐Liss, Inc.