bluuebunny/crossref_metadata_2025_split huggingface dataset:
The diagnostic performance of dual-layer spectral detector CT for distinguishing breast cancer biomarker expression and molecular subtypes
Authors: Chen Lanjing , Xiao Zhengyuan , Fu Jianmei , Huang Jingrong , Lan Yongshu 
Published: January 2024

AbstractTo evaluate the diagnostic performance of dual-layer spectral detector CT for differentiation of breast cancer molecular subtypes. This study was done in a retrospective approach including 104 female patients histopathologically proven to have breast cancer. These patients underwent chest arterial and venous phase dual-layer SDCT. CT values, iodine concentrations (IC)s, and Z-effective (Zeff) values of the lesions and arteries in the same layer were determined for both arterial and venous phases. Parameter values were normalized, and slopes of the spectral curves (λHu) were calculated. Breast cancer biomarkers were also analyzed. Afterward, correlations between the obtained parameters and biomarkers were analyzed. Eventually, the diagnostic performance was assessed using ROC curves. ER or PR-negative patients generally showed significantly higher mean iodine concentrations, CT, and Z-effective values. HER2-positive patients showed significantly higher CTVE, ZeffVE, N-ZeffVE, ICART, ICVE, NICART, NICVE, and λVE. Only ICVEand ZeffVEdiffered significantly between Ki67-positive and negative patients. All parameters showed significant diagnostic value for subtypes except N-ZeffART.Luminal and non-luminal types differed significantly and ROC curves indicated that multi-factors had the best diagnostic efficacy. The dual-layer SDCT distinguishes breast cancer biomarker expression and molecular subtypes. Thus, it can be used for preoperative assessment of breast cancer.
Critical analysis for nonlinear oscillations by least square HPM
Authors: Rafiq Muhammad , Kamran Muhammad , Ahmad Hijaz , Saliu Afis 
Published: January 2024

AbstractIn this study, a novel adapted homotopy perturbation method (HPM) is used to treat the nonlinear phenomena of free vibration in a system with one degree of freedom. This adaptation involves the integration of HPM with a least-squares optimizer, resulting in a hybrid method called the least square homotopy perturbation method (LSHPM). The LSHPM is tested on various nonlinear problems documented in the existing literature. To evaluate the effectiveness of the proposed approach, the identified problems are also tackled using HPM and the MATLAB built-in function bvp5c, and then the results are compared with those obtained using LSHPM. In addition, a comparative analysis is carried out with the results of the AG method as found in the literature. The results show that LSHPM is a reliable and efficient method suitable for solving more complicated initial value problems in the fields of science and engineering.
Spot urine sodium as a marker of urine dilution and decongestive abilities in acute heart failure
Authors: Guzik Mateusz , Iwanek Gracjan , Fudim Marat , Zymliński Robert , Marciniak Dominik , Ponikowski Piotr , Biegus Jan 
Published: January 2024

AbstractThe decongestion ability in response to diuretic treatment plays a crucial role in the treatment of acute heart failure. This effectiveness is evaluated through the assessment of sodium concentration and urine volume, which are also treatment goals themselves. However, the bidirectional interconnection between these factors remains not fully understood. The objective of this study is to provide mechanistic insights into the correlation between spot urine sodium concentrations (UNa+) and urine dilution. This aims to better understand of the decongestive abilities in acute heart failure (AHF). The study was single-center, prospective, conducted on a group of 50 AHF patients. Each participant received a standardized furosemide dose of 1 mg per kg of body weight. Hourly diuresis was measured in the first 6 h of the study, and urine composition was assessed at predefined timepoints. The study group presented the exponential (rather than linear) pattern of relationship between UNa+ and 6-h urine volume, whereas relationship between eGFR and 6-h urine volume was linear (r = 0.61, p < 0.001). The relationship between UNa+ and all other analyzed indices of urine dilution, including the change from baseline in urine creatinine concentration, urine osmolarity, and urine osmolarity corrected for urine sodium, also exhibited an exponential relationship. Patients who were chronically exposed to furosemide demonstrated a significantly lower urine dilution (1.78 [1.18–3.54] vs 11.58 [3.9–17.88]; p < 0.001) in comparison to naïve individuals. In conclusion, it should be noted that in AHF higher UNa+ is associated with disproportionally higher urine dilution, and patients naïve to furosemide have significantly greater ability to dilute urine when compare to chronic furosemide users.
Effect of histidine protonation state on ligand binding at the ATP-binding site of human protein kinase CK2
Authors: Winiewska-Szajewska Maria , Paprocki Daniel , Marzec Ewa , Poznański Jarosław 
Published: January 2024

AbstractHistidine residues contribute to numerous molecular interactions, owing to their structure with the ionizable aromatic side chain with pKa close to the physiological pH. Herein, we studied how the two histidine residues, His115 and His160 of the catalytic subunit of human protein kinase CK2, affect the binding of the halogenated heterocyclic ligands at the ATP-binding site. Thermodynamic studies on the interaction between five variants of hCK2α (WT protein and four histidine mutants) and three ionizable bromo-benzotriazoles and their conditionally non-ionizable benzimidazole counterparts were performed with nanoDSF, MST, and ITC. The results allowed us to identify the contribution of interactions involving the particular histidine residues to ligand binding. We showed that despite the well-documented hydrogen bonding/salt bridge formation dragging the anionic ligands towards Lys68, the protonated His160 also contributes to the binding of such ligands by long-range electrostatic interactions. Simultaneously, His 115 indirectly affects ligand binding, placing the hinge region in open/closed conformations.
LncRNA SOX9-AS1 triggers a transcriptional program involved in lipid metabolic reprogramming, cell migration and invasion in triple-negative breast cancer
Authors: Cisneros-Villanueva Mireya , Fonseca-Montaño Marco Antonio , Ríos-Romero Magdalena , López-Camarillo César , Jiménez-Morales Silvia , Langley Elizabeth , Rosette-Rueda Alan Sajid , Cedro-Tanda Alberto , Hernández-Sotelo Daniel , Hidalgo-Miranda Alfredo 
Published: January 2024

AbstractAt the molecular level, triple-negative breast cancer (TNBC) is frequently categorized as PAM50 basal-like subtype, but despite the advances in molecular analyses, the clinical outcome for these subtypes is uncertain. Long non-coding RNAs (lncRNAs) are master regulators of genes involved in hallmarks of cancer, which makes them suitable biomarkers for breast cancer (BRCA) diagnosis and prognosis. Here, we evaluated the regulatory role of lncRNA SOX9-AS1 in these subtypes. Using the BRCA-TCGA cohort, we observed that SOX9-AS1 was significantly overexpressed in basal-like and TNBC in comparison with other BRCA subtypes. Survival analyzes showed that SOX9-AS1 overexpression was associated with a favorable prognosis in TNBC and basal-like patients. To study the functions of SOX9-AS1, we determined the expression levels in a panel of nine BRCA cell lines finding increased levels in MDA-MB-468 and HCC1187 TNBC. Using subcellular fractionation in these cell lines, we ascertained that SOX9-AS1 was located in the cytoplasmic compartment. In addition, we performed SOX9-AS1 gene silencing using two short-harping constructs, which were transfected in both cell models and performed a genome-wide RNA-seq analysis. Data showed that 351 lncRNAs and 740 mRNAs were differentially expressed in MDA-MB-468 while 56 lncRNAs and 100 mRNAs were modulated in HCC1187 cells (Log2FC < - 1.5 and > 1.5, p.adj value < 0.05). Pathway analysis revealed that the protein-encoding genes potentially regulate lipid metabolic reprogramming, and epithelial–mesenchymal transition (EMT). Expression of lipid metabolic-related genes LIPE, REEP6, GABRE, FBP1, SCD1, UGT2B11, APOC1 was confirmed by RT-qPCR. Functional analysis demonstrated that the knockdown of SOX9-AS1 increases the triglyceride synthesis, cell migration and invasion in both two TNBC cell lines. In conclusion, high SOX9-AS1 expression predicts an improved clinical course in patients, while the loss of SOX9-AS1 expression enhances the aggressiveness of TNBC cells.
Analyzing the influential factors of process safety culture by hybrid hidden content analysis and fuzzy DEMATEL
Authors: Ghorbani Mohammad , Ebrahimi Hossein , Vosoughi Shahram , Eskandari Davoud , Moradi Hanifi Saber , Mandali Hassan 
Published: January 2024

AbstractDue to the complex nature of safety culture and process industries, several factors influence process safety culture. This paper presents a novel framework that combines the hidden content analysis method with Decision Making Trial and Evaluation Laboratory (DEMATEL) and Fuzzy logic to achieve a comprehensive set of influential factors and their relationship. The proposed methodology consists of two primary stages. Firstly, combined methods of literature review and Delphi study were used to identifying influential factors of process safety culture. Secondly, the Fuzzy-DEMATEL approach is employed to quantify and determine the relationships between different influential factors. A diverse pool of experts’ opinions is leveraged to assess the impact of each factor on others and process safety culture. In the first stage, 18 factors identified as influential factors on process safety. The findings of second stage revealed that eight variables were identified as causes, while ten variables were classified as effects. Also, the Organization management's commitment to safety factor had the greatest influence among all of the factors. As well as, the most significant interaction was associated with the risk assessment and management aspect. The integrated approach not only identified the influential factors, but also elucidates the cause-effect relationships among factors. By prioritizing factors and understanding their interconnections, organizations can implement targeted safety measures to improve process safety culture. Its effectiveness in quantifying qualitative data, identifying influential factors, and establishing cause-effect relationships make it a valuable tool for enhancing safety culture in process industries.
Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization
Authors: Bai Xinyi , Wang Yingxue , Ma Xinyuan , Yang Yingying , Deng Cong , Sun Mengling , Lin Chen , Zhang Linkun 
Published: January 2024

AbstractSeveral studies have demonstrated that exosomes (Exos) are involved in the regulation of macrophage polarization and osteoclast differentiation. However, the characteristics as well as roles of exosomes from human periodontal ligament cells (hPDLCs-Exos) in M1/M2 macrophage polarization and osteoclast differentiation remain unclear. Here, periodontal ligament cells were successfully extracted by method of improved Type-I collagen enzyme digestion. hPDLCs-Exos were extracted by ultracentrifugation. hPDLCs-Exos were identified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). Osteoclast differentiation was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR), WB and tartrate-resistant acid phosphatase (TRAP) staining. M1/M2 macrophage polarization were evaluated by RT-qPCR and WB. The results showed hPDLCs-Exos promoted osteoclast differentiation and M2 macrophage polarization, but inhibited M1 macrophage polarization. Moreover, M1 macrophages inhibited osteoclast differentiation, whereas M2 macrophages promoted osteoclast differentiation. It has shown that hPDLCs-Exos promoted osteoclast differentiation by inhibiting M1 and promoting M2 macrophage polarization.
Optimization of atmospheric leaching parameters for cadmium and zinc recovery from low-grade waste by response surface methodology (RSM)
Authors: Kolbadinejad Somayeh , Ghaemi Ahad 
Published: January 2024

AbstractThis study is focused on the optimization of effective parameters on Cadmium and Zinc recovery by atmospheric acid leaching of low-grade waste by response surface methodology (RSM) and using the Central Composite Design (CCD) method. The effects of parameters including time (0.5–2.5 h), temperature (40–80 °C), solid/liquid (S/L) (0.05–0.09 g/cc), particle size (174–44 mic), oxygen injection (0–1%) and pH (0.5–4.5) were statistically investigated at 5 surfaces. The sample of low-grade waste used in this study was mainly zinc factory waste. Two quadratic models for the correlation of independent parameters for the maximum recovery were proposed. The properties of waste were evaluated by X-ray diffraction (XRD) and X-ray fluorescence (XRF). Atomic absorption spectroscopy was used to determine the amount of Cadmium and Zinc in the leaching solution. The correlation coefficient (R2) for the predicted and experimental data of Cadmium and Zinc are 0.9837 and 0.9368, respectively. Time, S/L and size were the most effective parameters for the recovery efficiency of cadmium and zinc. 75.05% of Cadmium and 86.13% of Zinc were recovered in optimal conditions of leaching: S/L 0.08, pH 2.5, size 88 µm, 70 °C and 2.5 h. with air injection.
Reconstruction of lateral coherence and 2D emittance in plasma betatron X-ray sources
Authors: Curcio Alessandro , Cianchi Alessandro , Costa Gemma , Del Dotto Alessio , Demurtas Francesco , Ferrario Massimo , Frías Maria Dolores Rodríguez , Galletti Mario , Pérez-Hernández José Antonio , Gatti Giancarlo 
Published: January 2024

AbstractX-ray sources have a strong social impact, being implemented for biomedical research, material and environmental sciences. Nowadays, compact and accessible sources are made using lasers. We report evidence of nontrivial spectral-angular correlations in a laser-driven betatron X-ray source. Furthermore, by angularly-resolved spectral measurements, we detect the signature of spatial phase modulations by the electron trajectories. This allows the lateral coherence function to be retrieved, leading to the evaluation of the coherence area of the source, determining its brightness. Finally, the proposed methodology allows the unprecedented reconstruction of the size of the X-ray source and the electron beam emittance in the two main emission planes in a single shot. This information will be of fundamental interest for user applications of new radiation sources.
The Communicative Participation Item Bank–Gender-Diverse Version: Item Bank Calibration and Short Form
Authors: Baylor Carolyn , Bamer Alyssa , Brown Cait , Jin Jingyu Linna , Teixeira Jeffrey , Nuara Michael 
Published: January 2024

Purpose:
The purpose of this study was to calibrate an item set for a new version of the Communicative Participation Item Bank (CPIB) specifically for use with gender-diverse clients. This new version contains a new item stem as well as other minor wording changes from the original CPIB in order to be acceptable to gender-diverse respondents.

Method:
Survey data on 47 candidate items were collected from 434 transgender individuals: 219 assigned female at birth (AFAB) and 215 assigned male at birth (AMAB). Item response theory analyses included evaluation of unidimensionality, local dependence, fit to a graded response model, and differential item functioning (DIF) between AFAB and AMAB respondents.

Results:

The original set of 47 items was unidimensional, but 16 items were removed due to local dependence, resulting in a final item bank of 31 items. There was no evidence of DIF between AFAB and AMAB participants. Reliability of the full item bank is good (i.e., > 0.8) between
T
scores of 20 and 76 and high (i.e., > 0.9) between
T
scores of 20 and 68. The short form had good reliability (i.e., > 0.8) between
T
scores of 24 and 64.

Conclusions:
The Communicative Participation Item Bank–Gender-Diverse (CPIB-GD) version provides a new option for person-reported outcome measurement with gender-diverse clients. Clinicians are cautioned to use only the new CPIB-GD with gender-diverse clients, and not the original CPIB due to unacceptable wording in the original version for this population. The original CPIB remains valid and appropriate for the populations for which it was developed.

Supplemental Material:

https://doi.org/10.23641/asha.24993309
Behavioral Management of Respiratory/Phonatory Dysfunction for Dysarthria Associated With Neurodegenerative Disease: A Systematic Review
Authors: Perry Sarah E. , Troche Michelle , Huber Jessica E. , Curtis James , Kiefer Brianna , Sevitz Jordanna , Dennard Qiana , Borders James , Browy Jillian River , Dakin Avery , Gonzalez Victoria , Chapman Julianna , Wu Tiffany , Katz Lily , Britton Deanna 
Published: January 2024

Purpose:
This systematic review represents an update to previous reviews of the literature addressing behavioral management of respiratory/phonatory dysfunction in individuals with dysarthria due to neurodegenerative disease.

Method:
Multiple electronic database searches and hand searches of prominent speech-language pathology journals were conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses standards.

Results:
The search yielded 1,525 articles, from which 88 met inclusion criteria and were reviewed by two blinded co-investigators. A large range of therapeutic approaches have been added to the evidence base since the last review, including expiratory muscle strength training, singing, and computer- and device-driven programs, as well as a variety of treatment modalities, including teletherapy. Evidence for treatment in several different population groups—including cerebellar ataxia, myotonic dystrophy, autosomal recessive spastic ataxia of Charlevoix–Saguenay, Huntington's disease, multiple system atrophy, and Lewy body dementia—were added to the current review. Synthesis of evidence quality provided strong evidence in support of only one behavioral intervention: Lee Silverman Voice Treatment Program (LSVT LOUD) in people with Parkinson's disease. No other treatment approach or population included in this review demonstrated more than limited evidence, reflecting that these approaches/populations require urgent further examination.

Conclusion:
Suggestions about where future research efforts could be significantly strengthened and how clinicians can apply research findings to their practice are provided.

Supplemental Material:

https://doi.org/10.23641/asha.24964473
What School-Based Speech-Language Pathologists Need to Know About Pediatric Swallowing and Feeding Development and Disorders
Authors: Garcia Raquel 
Published: January 2024

Purpose:
School-based speech-language pathologists are at the frontline for treating children with pediatric feeding disorders (PFDs). Often, speech-language pathologists are the primary allied health professionals who are charged with maximizing safety, supporting nutrition and neurodevelopmental growth. Due to the increasing need for assessment and treatment of PFDs in schools, it is fundamental that PFDs and dysphagia are detected, differentially diagnosed, and addressed to support developing children. This tutorial addresses the anatomy, physiology, and development of functional feeding and swallowing patterns across the spectrum of childhood for both PFDs and dysphagia.

Method:
A clinical expert review of the anatomy, physiology, and development of functional feeding and swallowing patterns in children across the spectrum of childhood will be completed.

Conclusions:
The aim of this tutorial is to support school-based speech-language pathologists in their understanding of the anatomy and physiology of feeding and swallowing. School-based speech-language pathologists should complete this tutorial with an increased understanding and breadth of knowledge related to typical anatomy and physiology versus atypical or disordered presentations. This tutorial would support school-based speech-language pathologists in determining whether a child presents with characteristics of oropharyngeal dysphagia and/or a PFD.
Clinical, Anatomical, and Densitometric Changes following Dresden vs.
Accelerated Corneal Cross-Linking in Progressive Keratoconus
Authors: Prinz Julia , Kuerten David , Schellhase Hannah , Koutsonas Antonis , Djalali-Talab Yassin , Walter Peter , Fuest Matthias 
Published: January 2024

Abstract
Background To compare clinical, anatomical, and densitometric changes following
Dresden (DCXL) vs. accelerated (ACXL) corneal UVA cross-linking (CXL; Avedro KXL, Geuder,
Heidelberg, Germany) in progressive keratoconus (KC).
Methods and Material In this retrospective study, we analyzed 20 patients
following DCXL (3 mW/cm², 30 min, 5.4 J/cm²) and 44 patients following ACXL (9 mW/cm²,
10 min, 5.4 J/cm²) between January 2016 and February 2020. Uncorrected visual acuity
(UCVA), best spectacle-corrected visual acuity (BSCVA), central corneal thickness (CCT),
steepest keratometry (Kmax), keratoconus index (KI), thinnest pachymetry
(Pthin), and corneal densitometry (CD) were measured before and 3, 6, 12, and 24 months
after CXL.
Results During the follow-up period, no changes in UCVA, BSCVA, Kmax,
KI, or Pthin occurred. CCT significantly decreased 3 months after DCXL (p = 0.032) and
ACXL (p = 0.006). At the 12- and 24-month follow-up, CCT remained decreased in the DCXL
(p = 0.035, 0.036, respectively) but not in the ACXL group. At the 12-month follow-up, the
reduction in CCT was significantly greater in DCXL compared to ACXL (p = 0.012). At the
3-, 6-, 12-, and 24-month follow-ups, we found a significant increase in the anterior
stroma CD following DCXL (p = 0.019, 0.026, 0.049, 0.047, respectively) but not ACXL. The
CD changes were localized in the central concentric zones (0.0 to 6.0 mm). No intra- or
postoperative complications occurred.
Conclusion ACXL and DCXL effectively halted KC progression. ACXL proved to be a
safe time-saving alternative to conventional DCXL. DCXL led to a reduction in CCT and an
increment in the CD of the central anterior stroma during 24 months of follow-up.