bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observation of an exotic insulator to insulator transition upon electron doping the Mott insulator CeMnAsO
Authors: Wildman E. J. , Lawrence G. B. , Walsh A. , Morita K. , Simpson S. , Ritter C. , Stenning G. B. G. , Arevalo-Lopez A. M. , Mclaughlin A. C. 
Published: November 2023

AbstractA promising route to discover exotic electronic states in correlated electron systems is to vary the hole or electron doping away from a Mott insulating state. Important examples include quantum criticality and high-temperature superconductivity in cuprates. Here, we report the surprising discovery of a quantum insulating state upon electron doping the Mott insulator CeMnAsO, which emerges below a distinct critical transition temperature, TII. The insulator-insulator transition is accompanied by a significant reduction in electron mobility as well as a colossal Seebeck effect and slow dynamics due to decoupling of the electrons from the lattice phonons. The origin of the transition is tentatively interpreted in terms of many-body localization, which has not been observed previously in a solid-state material.
IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic murine Trypanosoma brucei infection
Authors: Sinton Matthew C. , Chandrasegaran Praveena R. G. , Capewell Paul , Cooper Anneli , Girard Alex , Ogunsola John , Perona-Wright Georgia , M Ngoyi Dieudonné , Kuispond Nono , Bucheton Bruno , Camara Mamadou , Kajimura Shingo , Bénézech Cécile , Mabbott Neil A. , MacLeod Annette , Quintana Juan F. 
Published: November 2023

AbstractIn the skin, Trypanosoma brucei colonises the subcutaneous white adipose tissue, and is proposed to be competent for forward transmission. The interaction between parasites, adipose tissue, and the local immune system is likely to drive the adipose tissue wasting and weight loss observed in cattle and humans infected with T. brucei. However, mechanistically, events leading to subcutaneous white adipose tissue wasting are not fully understood. Here, using several complementary approaches, including mass cytometry by time of flight, bulk and single cell transcriptomics, and in vivo genetic models, we show that T. brucei infection drives local expansion of several IL-17A-producing cells in the murine WAT, including TH17 and Vγ6+ cells. We also show that global IL-17 deficiency, or deletion of the adipocyte IL-17 receptor protect from infection-induced WAT wasting and weight loss. Unexpectedly, we find that abrogation of adipocyte IL-17 signalling results in a significant accumulation of Dpp4+Pi16+ interstitial preadipocytes and increased extravascular parasites in the WAT, highlighting a critical role for IL-17 signalling in controlling preadipocyte fate, subcutaneous WAT dynamics, and local parasite burden. Taken together, our study highlights the central role of adipocyte IL-17 signalling in controlling WAT responses to infection, suggesting that adipocytes are critical coordinators of tissue dynamics and immune responses to T. brucei infection.
A chromosome-level genome assembly of the Korean crossbred pig Nanchukmacdon (Sus scrofa)
Authors: Kwon Daehong , Park Nayoung , Wy Suyeon , Lee Daehwan , Chai Han-Ha , Cho In-Cheol , Lee Jongin , Kwon Kisang , Kim Heesun , Moon Youngbeen , Kim Juyeon , Park Woncheoul , Kim Jaebum 
Published: November 2023

AbstractAs plentiful high-quality genome assemblies have been accumulated, reference-guided genome assembly can be a good approach to reconstruct a high-quality assembly. Here, we present a chromosome-level genome assembly of the Korean crossbred pig called Nanchukmacdon (the NCMD assembly) using the reference-guided assembly approach with short and long reads. The NCMD assembly contains 20 chromosome-level scaffolds with a total size of 2.38 Gbp (N50: 138.77 Mbp). Its BUSCO score is 93.1%, which is comparable to the pig reference assembly, and a total of 20,588 protein-coding genes, 8,651 non-coding genes, and 996.14 Mbp of repetitive elements are annotated. The NCMD assembly was also used to close many gaps in the pig reference assembly. This NCMD assembly and annotation provide foundational resources for the genomic analyses of pig and related species.
Chromosome-level genome assembly and annotation of Zicaitai (Brassica rapa var. purpuraria)
Authors: Ren Hailong , Xu Donglin , Xiao Wanyu , Zhou Xianyu , Li Guangguang , Zou Jiwen , Zhang Hua , Zhang Zhibin , Zhang Jing , Zheng Yansong 
Published: November 2023

AbstractZicaitai is a seasonal vegetable known for its high anthocyanin content in both stalks and leaves, yet its reference genome has not been published to date. Here, we generated the first chromosome-level genome assembly of Zicaitai using a combination of PacBio long-reads, Illumina short-reads, and Hi-C sequencing techniques. The final genome length is 474.12 Mb with a scaffold N50 length of 43.82 Mb, a BUSCO score of 99.30% and the LAI score of 10.14. Repetitive elements accounted for 60.89% (288.72 Mb) of the genome, and Hi-C data enabled the allocation of 430.87 Mb of genome sequences to ten pseudochromosomes. A total of 42,051 protein-coding genes were successfully predicted using multiple methods, of which 99.74% were functionally annotated. Notably, comparing the genome of Zicaitai with seven other species in the Cruciferae family revealed strong conservation in terms of gene numbers and structures. Overall, the high-quality genome assembly provides a critical resource for studying the genetic basis of important agronomic traits in Zicaitai.
Microbial carbonate reservoir characteristics and their depositional effects, the IV Member of Dengying Formation, Gaoshiti-Moxi area, Sichuan Basin, Southwest China
Authors: Wang Yuan , Wang Shaoyong , Yan Haijun , Zhang Yijie , Zhao Zhenyu , Ma Debo 
Published: November 2023

AbstractPrevious scientific research on reservoirs of the Deng IV Member in the Gaoshiti-Moxi area, the main production area of the Anyue gas field with reserves of tens of billions of cubic metres, has focused on karst palaeogeomorphology reconstruction, the facies distribution on platform margins, and their effects on creating favourable reservoirs. However, the quality of microbial carbonate reservoirs is also closely related to their original depositional environments on both inner and marginal platforms. Therefore, this paper attempts to reveal favourable microbial carbonate reservoir characteristics and the sedimentary effects on their distribution and prediction based predominantly on a synthetic analysis of the sequence stratigraphy and depositional facies. The results show that favourable reservoirs of the Deng IV Member are classified into three types according to their reservoir spaces: fracture-cavity, pore-cavity and pore reservoirs. Secondary dissolution pores and cavities are primary reservoir spaces developed mainly in nonskeletal grain dolomites with sparry cements, thrombolites, and stromatolites. The physical properties of the fracture-cavity and pore-cavity types of reservoirs are better than those of pore reservoirs and have porosities between 1 and 5% and permeabilities between 0.01 × 10–3 and 1 × 10–3 μm2. Vertically, favourable reservoirs are developed mainly in parasequence set 6 (PSS6) and PSS7 and are laterally distributed in well zone MX9-MX19-MX1 for the fracture-cavity type, MX105-MX110-GS20 for the pore-cavity type and MX17-MX107-MX41-MX102-GS102 for the pore type. Moreover, depositional effects on reservoirs in terms of depositional sequences, seismic facies, microfacies and microfacies associations indicate that to some extent, the fracture-cavity type of reservoir is constrained by the top boundaries of PSS7, PSS2, parasequence 17 (PS17) and PS14; the pore-cavity type of reservoir is constrained by the top boundaries of PSS7, PSS4, PS18 and PS12; and the pore type of reservoir is constrained by the top boundaries of PSS7, PSS6, PSS3, PSS2, PS18, PS17, PS14, and PS12. Seismic facies associated with shoals and mound-flat complexes are related to the facies distributions of pore cavities and pore reservoirs. MA1, MA3, MA7, and MA8 are predominant microfacies associations of favourable reservoirs of the Deng IV Member in the Gaoshiti-Moxi area. The above results are significant for further petroleum exploration and exploitation of ultradeep microbial carbonate reservoirs in this area.
Natural killer cell responses during SARS-CoV-2 infection and vaccination in people living with HIV-1
Authors: Alrubayyi Aljawharah , Touizer Emma , Hameiri-Bowen Dan , Charlton Bethany , Gea-Mallorquí Ester , Hussain Noshin , da Costa Kelly A. S. , Ford Rosemarie , Rees-Spear Chloe , Fox Thomas A. , Williams Ian , Waters Laura , Barber Tristan J. , Burns Fiona , Kinloch Sabine , Morris Emma , Rowland-Jones Sarah , McCoy Laura E. , Peppa Dimitra 
Published: November 2023

AbstractNatural killer (NK) cell subsets with adaptive properties are emerging as regulators of vaccine-induced T and B cell responses and are specialized towards antibody-dependent functions contributing to SARS-CoV-2 control. Although HIV-1 infection is known to affect the NK cell pool, the additional impact of SARS-CoV-2 infection and/or vaccination on NK cell responses in people living with HIV (PLWH) has remained unexplored. Our data show that SARS-CoV-2 infection skews NK cells towards a more differentiated/adaptive CD57+FcεRIγ− phenotype in PLWH. A similar subset was induced following vaccination in SARS-CoV-2 naïve PLWH in addition to a CD56bright population with cytotoxic potential. Antibody-dependent NK cell function showed robust and durable responses to Spike up to 148 days post-infection, with responses enriched in adaptive NK cells. NK cell responses were further boosted by the first vaccine dose in SARS-CoV-2 exposed individuals and peaked after the second dose in SARS-CoV-2 naïve PLWH. The presence of adaptive NK cells associated with the magnitude of cellular and humoral responses. These data suggest that features of adaptive NK cells can be effectively engaged to complement and boost vaccine-induced adaptive immunity in potentially more vulnerable groups such as PLWH.
Investigation of single and multiple MPPT structures of solar PV-system under partial shading conditions considering direct duty-cycle controller
Authors: Youssef Abdel-Raheem , Hefny Mostafa M. , Ali Ahmed Ismail M. 
Published: November 2023

AbstractPartial shading of solar panels diminishes their operating efficiency and energy synthesized as it disrupts the uniform absorption of sunlight. To tackle the issue of partial shading in photovoltaic (PV) systems, this article puts forward a comprehensive control strategy that takes into account a range of contributing factors. The proposed control approach is based on using multi-string PV system configuration in place of a central-type PV inverter for all PV modules with a single DC-DC converter. This adaptation enhances overall efficiency across varying radiation levels. Also, the proposed technique minimizes the overall system cost by reducing the required sensors number by utilizing a radiation estimation strategy. The converter switching strategy is synthesized considering direct duty-cycle control method to establish the maximum power point (MPP) location on the P–V curve. The direct duty-cycle tracking approach simplifies the control system and improves the system’s response during sudden partial shading restrictions. To validate the effectiveness of the suggested MPPT method, two system configurations were constructed using MATLAB/SIMULINK software and assessed under various partial shading scenarios. Additionally, a multi-string system was subjected to real irradiance conditions. The sensor-less MPPT algorithm proposed achieved an impressive system efficiency of 99.81% with a peak-to-peak ripple voltage of 1.3V. This solution offers clear advantages over alternative approaches by reducing tracking time and enhancing system efficiency. The system findings undoubtedly support the theoretical scrutiny of the intended technique.
Significant influence of four highly conserved amino-acids in lipochaperon-active sHsps on the structure and functions of the Lo18 protein
Authors: Bellanger Tiffany , da Silva Barreira David , Wien Frank , Delarue Patrice , Senet Patrick , Rieu Aurélie , Neiers Fabrice , Varela Paloma Fernández , Combet Sophie , Weidmann Stéphanie 
Published: November 2023

AbstractTo cope with environmental stresses, bacteria have developed different strategies, including the production of small heat shock proteins (sHSP). All sHSPs are described for their role as molecular chaperones. Some of them, like the Lo18 protein synthesized by Oenococcus oeni, also have the particularity of acting as a lipochaperon to maintain membrane fluidity in its optimal state following cellular stresses. Lipochaperon activity is poorly characterized and very little information is available on the domains or amino-acids key to this activity. The aim in this paper is to investigate the importance at the protein structure and function level of four highly conserved residues in sHSP exhibiting lipochaperon activity. Thus, by combining in silico, in vitro and in vivo approaches the importance of three amino-acids present in the core of the protein was shown to maintain both the structure of Lo18 and its functions.
Synergistic effect of biochar-based compounds from vegetable wastes and gibberellic acid on wheat growth under salinity stress
Authors: Anwar Tauseef , Munwwar Fahmida , Qureshi Huma , Siddiqi Ejaz Hussain , Hanif Asma , Anwaar Sadaf , Gul Sarah , Waheed Abdul , Alwahibi Mona S. , Kamal Asif 
Published: November 2023

AbstractSoil salinization is a prevalent form of land degradation particularly in water-deficient regions threatening agricultural sustainability. Present desalinization methods demand excessive water use. Biochar has been recognized as a potential remedy for saline soils and Gibberellic acids (GA3) are known to mediate various biochemical processes aiding in stress mitigation. This study was undertaken at The Islamia University of Bahawalpur during winter 2022–23 to explore the combined effect of biochar and GA3 on wheat (Triticum aestivum L.) in saline conditions. Employing a fully randomized design wheat seeds in 24 pots were subjected to two salinity levels with three replications across eight treatments: T1 to T8 ranging from controls with different soil electrical conductivities (ECs) to treatments involving combinations of GA3, biochar and varying soil ECs. These treatments included T1 (control with soil EC of 2.43dS/m), T2 (salinity stress with soil EC of 5.11dS/m), T3 (10 ppm GA3 with soil EC of 2.43dS/m), T4 (10 ppm GA3 with soil EC of 5.11dS/m), T5 (0.75% Biochar with soil EC of 2.43dS/m), T6 (0.75% Biochar with soil EC of 5.11dS/m), T7 (10 ppm GA3 combined with 0.75% biochar at soil EC of 2.43dS/m) and T8 (10 ppm GA3 plus 0.75% biochar at soil EC of 5.11dS/m). The results indicated that the combined applications of GA3 and biochar significantly enhanced plant growth in saline conditions viz. germination rate by 73%, shoot length of 15.54 cm, root length of 4.96 cm, plant height of 16.89 cm, shoot fresh weight 43.18 g, shoot dry weight 11.57 g, root fresh weight 24.26 g, root dry weight 9.31 g, plant water content 60.77%, photosynthetic rate 18.58(CO2 m−2 s−1) carotenoid 3.03 g, chlorophyll a 1.01 g, chlorophyll b 0.69 g, total chlorophyll contents by 1.9 g as compared to the control. The findings suggest that the combined application of these agents offers a sustainable and effective strategy for cultivating wheat in saline soils. The synergy between biochar and GA3 presents a promising avenue for sustainable wheat cultivation in saline conditions. This combined approach not only improves plant growth but also offers an innovative, water-efficient solution for enhancing agricultural productivity in saline-affected regions.
In situ profiling reveals metabolic alterations in the tumor microenvironment of ovarian cancer after chemotherapy
Authors: Corvigno Sara , Badal Sunil , Spradlin Meredith L. , Keating Michael , Pereira Igor , Stur Elaine , Bayraktar Emine , Foster Katherine I. , Bateman Nicholas W. , Barakat Waleed , Darcy Kathleen M. , Conrads Thomas P. , Maxwell G. Larry , Lorenzi Philip L. , Lutgendorf Susan K. , Wen Yunfei , Zhao Li , Thaker Premal H. , Goodheart Michael J. , Liu Jinsong , Fleming Nicole , Lee Sanghoon , Eberlin Livia S. , Sood Anil K. 
Published: November 2023

AbstractIn this study, we investigated the metabolic alterations associated with clinical response to chemotherapy in patients with ovarian cancer. Pre- and post-neoadjuvant chemotherapy (NACT) tissues from patients with high-grade serous ovarian cancer (HGSC) who had poor response (PR) or excellent response (ER) to NACT were examined. Desorption electrospray ionization mass spectrometry (DESI-MS) was performed on sections of HGSC tissues collected according to a rigorous laparoscopic triage algorithm. Quantitative MS-based proteomics and phosphoproteomics were performed on a subgroup of pre-NACT samples. Highly abundant metabolites in the pre-NACT PR tumors were related to pyrimidine metabolism in the epithelial regions and oxygen-dependent proline hydroxylation of hypoxia-inducible factor alpha in the stromal regions. Metabolites more abundant in the epithelial regions of post-NACT PR tumors were involved in the metabolism of nucleotides, and metabolites more abundant in the stromal regions of post-NACT PR tumors were related to aspartate and asparagine metabolism, phenylalanine and tyrosine metabolism, nucleotide biosynthesis, and the urea cycle. A predictive model built on ions with differential abundances allowed the classification of patients’ tumor responses as ER or PR with 75% accuracy (10-fold cross-validation ridge regression model). These findings offer new insights related to differential responses to chemotherapy and could lead to novel actionable targets.
Identification of circulating proteins associated with general cognitive function among middle-aged and older adults
Authors: Tin Adrienne , Fohner Alison E. , Yang Qiong , Brody Jennifer A. , Davies Gail , Yao Jie , Liu Dan , Caro Ilana , Lindbohm Joni V. , Duggan Michael R. , Meirelles Osorio , Harris Sarah E. , Gudmundsdottir Valborg , Taylor Adele M. , Henry Albert , Beiser Alexa S. , Shojaie Ali , Coors Annabell , Fitzpatrick Annette L. , Langenberg Claudia , Satizabal Claudia L. , Sitlani Colleen M. , Wheeler Eleanor , Tucker-Drob Elliot M. , Bressler Jan , Coresh Josef , Bis Joshua C. , Candia Julián , Jennings Lori L. , Pietzner Maik , Lathrop Mark , Lopez Oscar L. , Redmond Paul , Gerszten Robert E. , Rich Stephen S. , Heckbert Susan R. , Austin Thomas R. , Hughes Timothy M. , Tanaka Toshiko , Emilsson Valur , Vasan Ramachandran S. , Guo Xiuqing , Zhu Yineng , Tzourio Christophe , Rotter Jerome I. , Walker Keenan A. , Ferrucci Luigi , Kivimäki Mika , Breteler Monique M. B. , Cox Simon R. , Debette Stephanie , Mosley Thomas H. , Gudnason Vilmundur G. , Launer Lenore J. , Psaty Bruce M. , Seshadri Sudha , Fornage Myriam 
Published: November 2023

AbstractIdentifying circulating proteins associated with cognitive function may point to biomarkers and molecular process of cognitive impairment. Few studies have investigated the association between circulating proteins and cognitive function. We identify 246 protein measures quantified by the SomaScan assay as associated with cognitive function (p < 4.9E-5, n up to 7289). Of these, 45 were replicated using SomaScan data, and three were replicated using Olink data at Bonferroni-corrected significance. Enrichment analysis linked the proteins associated with general cognitive function to cell signaling pathways and synapse architecture. Mendelian randomization analysis implicated higher levels of NECTIN2, a protein mediating viral entry into neuronal cells, with higher Alzheimer’s disease (AD) risk (p = 2.5E-26). Levels of 14 other protein measures were implicated as consequences of AD susceptibility (p < 2.0E-4). Proteins implicated as causes or consequences of AD susceptibility may provide new insight into the potential relationship between immunity and AD susceptibility as well as potential therapeutic targets.
Working memory performance is tied to stimulus complexity
Authors: Pusch Roland , Packheiser Julian , Azizi Amir Hossein , Sevincik Celil Semih , Rose Jonas , Cheng Sen , Stüttgen Maik C. , Güntürkün Onur 
Published: November 2023

AbstractWorking memory is the cognitive capability to maintain and process information over short periods. Behavioral and computational studies have shown that visual information is associated with working memory performance. However, the underlying neural correlates remain unknown. To identify how visual information affects working memory performance, we conducted behavioral experiments in pigeons (Columba livia) and single unit recordings in the avian prefrontal analog, the nidopallium caudolaterale (NCL). Complex pictures featuring luminance, spatial and color information, were associated with higher working memory performance compared to uniform gray pictures in conjunction with distinct neural coding patterns. For complex pictures, we found a multiplexed neuronal code displaying visual and value-related features that switched to a representation of the upcoming choice during a delay period. When processing gray stimuli, NCL neurons did not multiplex and exclusively represented the choice already during stimulus presentation and throughout the delay period. The prolonged representation possibly resulted in a decay of the memory trace ultimately leading to a decrease in performance. In conclusion, we found that high stimulus complexity is associated with neuronal multiplexing of the working memory representation possibly allowing a facilitated read-out of the neural code resulting in enhancement of working memory performance.