bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum ESPL1 Protein as an Early Warning Biomarker for the Initial Occurrence and Recurrence of Hepatitis B Virus-Related Hepatocellular Carcinoma
Authors: Hu Bobin , Deng Deli , Liang Hengkai , Wang Rongming , Su Minghua , Wei Lu , Su Tumei , Li Qingmei , Yin Qianbing , Feng Yanfei , Jiang Jianning 
Published: October 2023

Abstract
Aim This study aims to assess the potential of serum Extra Spindle Pole Bodies-like 1 (ESPL1) protein as a biomarker for the effective early warning of Hepatitis B Virus (HBV)-related hepatocellular carcinoma (HCC).
Methods A cross-sectional study was conducted, including 119 patients who were classified into three groups: chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC), and HBV-HCC. Additionally, a longitudinal study included 49 patients in a non-recurrent (N-Rec) group with first occurrence of HBV-HCC and a recurrent (Rec) group with multiple HCC recurrences. Serum ESPL1 levels were measured using enzyme-linked immunosorbent assay. Differences in serum ESPL1 levels among the CHB, HBV-LC, and HBV-HCC groups and alpha-fetoprotein (AFP) levels among these groups were compared. The dynamic changes of serum ESPL1 and AFP levels were analyzed in the N-Rec group before and after the hepatectomy and in the Rec group before and after each HCC recurrence.
Results Serum ESPL1 levels showed a gradual increase in the CHB, HBV-LC, and HBV-HCC groups (P<0.05). In the N-Rec group, serum ESPL1 levels exhibited a continuous upward trend in the 5 years before the hepatectomy, followed by a consistent decline over the subsequent 5 years. In the Rec group, serum ESPL1 levels demonstrated a fluctuating pattern of increase and decrease before and after each liver cancer resection. Conversely, the changes in serum AFP levels did not follow a consistent pattern.
Conclusion Serum ESPL1 has potential as a biomarker for effective early warning of initial and recurrent HBV-related HCC.
Correlation between body mass index and perianal abscess hazards: a Mendelian randomization investigation
Authors: Saad Hassan A. , Farid Mohamed I , Eraky Mohamed E , El-taher Ahmed k , Sharaf Khaled , Baz Azza , Riad Mohamed 
Published: October 2023

Abstract
Background:Perianal abscess is an infectious disease that frequently affects the perianal region. Body mass index (BMI) is a commonly used statistic to assess weight and weight gain. Although there is evidence linking obesity to several medical conditions, the relationship between BMI and perianal abscesses remains unclear. Whether BMI influences the likelihood of perianal abscess remains unclear.
Methods
A two-sample Mendelian randomization (MR) study was conducted using weighted average, weighting the median, weighted inverse-variance weighted (IVW), and MR-Egger regression approaches. Using the accessible to everybody summarized collections of GWAS meta-analyses for BMI among people of South Asian descent (n = 8,658) as being exposed, a genome-wide association experiment (GWAS) for anal and rectal abscess from people who are involved in the OpenGWAS database (total n = 183 710; case = 1 287, control = 182 423) was used as the outcome.
Results:
We used GWASs to find 29 single nucleotide polymorphisms (SNPs) of genome-wide relevance, using BMI as the variable in question. The IVW technique indicateds that there was not enough information to determine a cause-and-effect link between BMI and perianal abscess (Beta = -0.093, SE = 0.097, P = 0.340). An analysis of MR-Egger regression revealed a lack of causality between BMI and perianal abscess (beta = -0.254, SE = 0.177, P = 0.162). The analysis also showed that directed multiplication did not influence the results (intercept = 0.024; P = 0.285). Furthermore, there is no evidence that a link between BMI and perianal abscess exists according to the weighted mean (Beta = -0.207, SE = 0.182, P = 0.813) or weighted median (Beta = -0.126, SE = 0.139, P = 0.363). The funnel plot and Cochran's Q test, which also revealed no evidence of diversity or imbalances, demonstrated that there was no directional pleiotropy.
In summary, there were insufficient data from the MR analysis to substantiate the theory linking a greater BMI with a higher incidence of perianal abscess.
Optimization of Clostridium beijerinckii Semi-solid Fermentation of Rape Straw to Produce Butyric acid by Genome Analysis
Authors: Kou Hui , Zheng Jia , Ye Guangbin , Qiao Zongwei , Zhang Kaizheng , Luo Huibo , Zou Wei 
Published: October 2023

Abstract
Butyric acid is a volatile saturated monocarboxylic acid, which is widely used in the chemical, food, pharmaceutical, energy, and animal feed industries. This study focuses on producing butyric acid from pre-treated rape straw using simultaneous enzymatic hydrolysis semi-solid fermentation (SEHSF). Clostridium beijerinckii BRM001 screened from pit mud of Chinese nongxiangxing baijiu was used. The genome of C. beijerinckii BRM001 was sequenced and annotated. Using rape straw as the sole carbon source, fermentation optimization was carried out based on the genomic analysis of BRM001. The optimized butyric acid yield was as high as 13.86 ± 0.77 g/L, which was 2.1 times higher than that of the initial screening. Furthermore, under optimal conditions, non-sterile SEHSF was carried out, and the yield of butyric acid was 13.42 ± 0.83 g/L in a 2.5-L fermentor. This study provides a new approach for butyric acid production which eliminates the need for detoxification of straw hydrolysate and makes full use of the value of fermentation waste residue without secondary pollution, making the whole process greener and more economical, which has a certain industrial potential.
E-Pharmacophore modelling, molecular docking and dynamics approaches for in silico identification of acetylcholinesterase inhibitors from natural products against Alzheimer’s disease
Authors: Ramachandran Anu Kunnath , Birangal Sumit Raosaheb , Das Subham , Ranadive Niraja , Gautham Shenoy Gurupur , Bhat Varadaraj , Fayaz S M , Mudgal Jayesh , Joseph Alex 
Published: October 2023

Abstract
Alzheimer's disease (AD) is the most common cause of dementia and one of the major causes of death worldwide. The ability of some natural compounds, such as flavonoids, to cross the blood-brain barrier and decrease the progression of such disorders has been described in the literature. This study aimed to identify potential natural product molecules as acetylcholinesterase inhibitors of Alzheimer's disease using silico computational approaches. A pharmacophore model was developed based on the 3D structure of the protein–ligand complex of the acetylcholinesterase protein (PDB:4EY7) using the Phase module. A natural product library of 30,926 ligands was prepared using LigPrep and was used for virtual screening. Based on the pharmacophore similarity score, the best ligands were identified and further scrutinized by molecular docking, MM/GBSA, induced fit docking, and ADME profiling. Two of the most promising natural products, NPC109925 and NPC170602, were evaluated to understand the stability of these ligands in the binding pocket using molecular dynamics (MD) simulation for 100ns and post dynamic MM/GBSA was performed for 101 frames from MD simulation result which showed better binding energy in comparison with pre dynamic MM/GBSA. These molecules exhibited better binding affinity compared to the co-crystallized ligand in in silico studies. However, further in vitro and in vivo screening is recommended to confirm the acetylcholinesterase inhibitory activity of these ligands which could serve as lead molecules for further development as anti- Alzheimer’s agents.
Surgical Outcomes of Robotic Thyroidectomy for Thyroid Tumors over 4 cm via the Bilateral Axillo-Breast Approach
Authors: Bae Hye Lim , Wong Junice Shi-Hui , Kim Su-jin , Jung Younghoon , Choi Jaebong , Kwak JungHak , Yu Hyeong Won , Chai Young Jun , Choi June Young , Lee Kyu Eun 
Published: October 2023

Abstract

The study investigated the feasibility of robotic bilateral axillo-breast approach (BABA) thyroidectomy for patients with thyroid tumors larger than 4 cm. BABA thyroidectomy has previously shown safety and effectiveness for thyroid surgeries but lacked extensive data on its application to larger tumors. Between October 2008 and August 2022, 74 patients underwent robotic BABA thyroidectomy. The mean patient age was 40.3 years. Fine needle aspiration results classified the tumors as benign (45.9%), atypia of undetermined significance (20.3%), follicular neoplasm (27.0%), suspicious for malignancy/malignancy (5.4%), or lymphoma (1.4%). The average tumor size was 4.9 cm, with the majority (85.1%) undergoing thyroid lobectomy, and the rest (14.9%) receiving total thyroidectomy. The mean total operation time was 178.4 minutes for lobectomy and 207.3 minutes for total thyroidectomy. Transient vocal cord palsy (VCP) was found in 3 patients (3.5%), and there was no permanent VCP. Among patients who underwent total thyroidectomy, transient hypoparathyroidism was observed in three (27.2%), and permanent hypoparathyroidism was observed in one (9.1%). There were no cases of open conversion, tumor spillage, bleeding, flap injury, or tumor recurrence. In conclusion, robotic BABA thyroidectomy may be a safe treatment option for large-sized thyroid tumors that carries no significant increase in complication rates.
Human neural stem cell secretome relieves endoplasmic reticulum stress-induced apoptosis and improves neuronal functions after traumatic brain injury in a rat model
Authors: Ling Yating , Ramalingam Murugan , Lv Xiaorui , Niu Dongdong , Zeng Yu , Qiu Yun , Si Yu , Guo Tao , Ni Yinying , Zhang jingwen , Wang Ziyu , Hu Jiabo 
Published: October 2023

Abstract
Neural stem cell secretome (NSC-S) plays an important role in neuroprotection and recovery. Studies have shown that endoplasmic reticulum stress (ER stress) is involved in the progression of traumatic brain injury (TBI) and is a crucial cause of secondary damage and neuronal death after brain injury. Whether NSC-S is engaged in ER stress and ER stress-mediated neuronal apoptosis post-TBI has not been investigated. In the study, the Feeney SD male rat model was established. The results indicated that NSC-S treatment could significantly improve the behavior of TBI rats and reduce the area of brain contusion in rats. In addition, NSC-S relieved ER stress in TBI rats and was observed by transmission electron microscopy and western blot. The specific mechanism was further elucidated that restoration was achieved by alleviating the PERK-eIF2α pathway and thus protecting neurons from apoptosis. Notably, the discovery of calumenin (CALU) in NSC-S by liquid chromatography-tandem mass spectrometry (LC-MS/MS/MS) may be related to the protective effect of NSC-S on ER stress in neurons. Also, the mechanism by which it functions may be related to ubiquitination. In summary, NSC-S improved prognosis and ER stress in TBI rats and might be a promising treatment for relieving TBI.
Tuning in to Emotion: Prosodic Discrimination Skills Mediate the Association Between Musical Aptitude and Vocal Emotion Recognition Ability
Authors: Vigl Julia , Talamini Francesca , Strauß Hannah , Zentner Marcel 
Published: October 2023

Abstract

The current study tested the hypothesis that the association between musical ability and vocal emotion recognition skills is mediated by accuracy in prosody perception. Furthermore, it was investigated whether this association is primarily related to musical expertise, operationalized by long-term engagement in musical activities, or musical aptitude, operationalized by a test of musical perceptual ability. To this end, we conducted three studies: In Study 1 (N = 85) and Study 2 (N = 93), we developed and validated a new instrument for the assessment of prosodic discrimination ability. In Study 3 (N = 136), we examined whether the association between musical ability and vocal emotion recognition was mediated by prosodic discrimination ability. We found evidence for a full mediation, though only in relation to musical aptitude and not in relation to musical expertise. Taken together, these findings suggest that individuals with high musical aptitude have superior prosody perception skills, which in turn contribute to their vocal emotion recognition skills. Importantly, our results suggest that these benefits are not unique to musicians, but extend to non-musicians with high musical aptitude.
Prognostic and predictive value of a mRNA signature in high-grade serous ovarian carcinoma with an integrated computation analysis
Authors: Yang Fanchun , zhou Yang , Liao Caihe 
Published: October 2023

Abstract
Ovarian cancer (OC) is the leading cause of death among women with gynecologic malignancies. High-grade serous ovarian carcinoma (HGSOC) is the deadliest subtype of OC, accounting for about 90 percent of all ovarian cancer subtypes. Recent studies have shown that HGSOC patients have mutations in proto-oncogenes within the genome. Genome-wide detection and diagnosis are helpful for the diagnosis and treatment of HGSOC. To explore the genomic and transcriptional characteristics of subtypes of HGSOC, achieve accurate typing of tumor types, and obtain genomic characteristics that can reflect the subtypes of HGSOC, Using NMF clustering, SAM, PAM and survival time analysis, copy number variation data and gene expression data of 698 HGSOC samples were analyzed and differential expression genes of different disease subtypes were enriched and analyzed. Functions of genes related to different disease subtypes were enriched. All patients with HGSOC could be stratified into three categories according to genetic variation information and gene expression value. There was significant difference in the survival time curves of patients in different subtypes. And we identified twenty-one genes as the ones with the strongest power to differentiate the samples, including FTH1, COL1A2, COL3A1, GFBP7, ACTB, SPARC, PTTG1IP, TIMP1 and HLA-DPA1. Furthermore, we found that JAK/STAT (Janus kinase and signal transducers and activators of transcription) signaling pathway changes obviously in different subtypes. By investigating the genetic features and gene expression features, subtypes of patients with HGSOC could be accurately judged, which is useful for selecting therapeutic methods.
Bacteremia from Streptococcus constellatus revealing a Gastro-Intestinal Stromal Tumor
Authors: Chessa Salvatore , Belfiori Elena , Mandis Giulia , Urru Enrico , Manconi Giovanna , Scuteri Angelo 
Published: October 2023

Abstract
Background. Pyogenic Liver Abscesses (PLA) are the most common type of visceral abscess. They generally develop in a context of biliary disease or hematogenous seeding, but a complete diagnostic work-up is always required in order not to miss other important causes, including above all malignancies of the gastro-intestinal tract.
Case presentation. Herein, we report a particular case of a 80 years-old immunocompetent woman hospitalized for sepsis. At the end of the diagnostic process, Streptococcus constellatus(Sc) was identified as the cause of sepsis, multiple PLA were found together with a previous unknown ileal malignancy. We speculated about a possible correlation among these three entities (i.e. sepsis from Sc, PLA and tumors).
Conclusions. Detection of Streptococcus constellatus in blood should raise red flags in clinicians as aggressive clinical presentation are possible.
What is the impact of Kinesotaping on anterior cruciate ligament reconstruction: a systematic review and meta-analysis.
Authors: Elrosasy Amr , Zeid Mohamed Abo , Abbas Ahmed W. , Eldeeb Hatem , Eljadid Ghaith Y. , Hamid Abdulrahman Khaldoon 
Published: October 2023

Abstract
Background:
For individuals with ACL injuries, anterior cruciate ligament (ACL) reconstruction is a frequent surgical technique. Restoration of knee function and excellent outcomes depend heavily on postoperative therapy. As a therapeutic intervention for several musculoskeletal illnesses, the benefits and effectiveness of kinesio taping are currently unknown. This systematic review and meta-analysis's goal is to evaluate the effectiveness of kinesio taping for ACL reconstruction and its impact on clinical outcomes.
Methods:
A comprehensive search of PubMed, EMBASE, SCOPUS, and Web of Science was done to discover relevant studies. Inclusion criteria included controlled or randomized clinical trials that were published in English. Changes in pain, flexion strength, and extension strength were among the outcomes of interest. RevMan 5.4 was used to extract and analyze data.
Results:
After satisfying the inclusion requirements, five studies were included in the systematic review. Pooled analysis showed that, in comparison to the intervention group, the control group had a statistically significant improvement in flexion strength (SMD = 0.44, 95% CI [0.01, 0.87], P=0.04). Extension strength and pain, however, did not significantly differ between the intervention and control groups (SMD = 0.26, 95% CI [-0.14, 0.66], P=0.20; 95% CI [-0.12, 0.72], P=0.16). For flexion and extension strength, the pooled trials revealed homogeneity (P=0.33, I-square=9%; P=0.67, I-square= 0%, respectively), while heterogeneity was observed for the pain outcome (P=0.02, I-square= 75%).
Conclusions:
This analysis suggests limited to no benefits of kinesio taping post-ACL reconstruction. While the control group surprisingly showed better improvement in flexion strength, no significant differences were found in extension strength and pain. Further rigorous trials are needed to confirm its utility in rehabilitation.
Tunable Ultra-Broadband Terahertz Metamaterial Absorbers Based on Complementary Split Ring-Shaped Graphene
Authors: Ri Kwang-Jin , Kim Pyong , Ri Chung-Ho 
Published: October 2023

Abstract

In the design of tunable broadband terahertz (THz) metamaterial absorbers based on graphene, simplifying the gating structure to control the Fermi energy of graphene is urgently required for practical applications. Pursuing this demand, we propose two kinds of tunable ultra-broadband THz metamaterial absorbers based on complementary split ring-shaped graphene. The first absorber can achieve an ultra-broadband absorption performance with absorptivity above 90% in the range of 2.06–4.24 THz and its relative absorption bandwidth is 69.2%. By varying the Fermi energy of graphene from 0 eV to 0.8 eV via bias voltage, the absorptivity can be dynamically tuned from 32.8–99.9%. The physical mechanism of ultra-broadband absorption is based on the surface plasmon polariton resonances excited by the surface charges of complementary split ring-shaped graphene. In addition, to further expand the absorption bandwidth, the first absorber structure is covered with another dielectric layer, resulting in the second absorber with enhanced relative absorption bandwidth of 108.27%. Our designed absorbers have many potential applications such as medical imaging, explosive detection, biological sensing and wireless communications.
Experimental Analysis of Low-Velocity Impact and CAI Properties of 3D four-directional Braided Composites after Hygrothermal Aging
Authors: Zhang Yuxuan , Yan Shi , Du Changmei , Jiang Lili , Zhai Junjun 
Published: October 2023

Abstract
In this paper, 3D four-directional braided composites(3D4d-BC) materials with different braiding angles (15°and 30°) were subjected to hygrothermal aging treatment under different conditions, and then low-velocity impact and post-impact compression experiments were conducted to record the changes of relevant parameters. Through experiments, the temperature has an important effect on the increase of water absorption of the epoxy resin matrix. Higher water absorption results in better energy absorption for 3D4d-BC, but it is less integrated and therefore has lower residual compression properties. Although the intervention of oxygen molecules has little effect on the water absorption of the matrix, it has the degraded interface property between the matrix and the composite material, so it will further reduce its compression strength. The smaller the braiding Angle, the lower the water absorption efficiency and the lower the impact peak load during the hygrothermal aging process. In the compression process, H15 occurs mainly along the lateral side, and the failure form is buckling failure. H30 fractures along the 45° direction, and the failure form is a shear failure.
IVIg for recurrent implantation failure: the right treatment for the right patient?
Authors: Peero Einav Kadour , Banjar Shorooq , Khoudja Rabea , Ton-leclerc Shaonie , Beauchamp Coralie , Benoit Joanne , Beltempo Marc , Dahan Michael H. , Gold Phil , Kadoch Isaac Jacques , Jamal Wael , Laskin Carl , Mahutte Neal , Phillips Simon , Sylvestre Camille , Reinblatt Shauna , Mazer Bruce D. , Buckett William , Genest Genevieve 
Published: October 2023

Abstract
The effectiveness of intravenous immunoglobulin (IVIg) for patients with unexplained recurrent implantation failure (uRIF) remains debated. The objective of this study was to evaluate outcomes in patients with uRIF treated with intravenous immunoglobulin (IVIg) compared to a separate cohort of uRIF patients not receiving IVIg within our center. We performed a retrospective cohort study defining uRIF as \(\ge\) 3 unexplained previously failed high quality blastocyst transfer failures in patients with a body mass index < 35, aged < 42, non-smoking, with >7mm type I endometrium at time of transfers. Primary outcomes included live birth, miscarriage, or transfer failure. We documented IVIg side effects and maternal/fetal outcomes. Logistic regression analysis was used to assess for association of IVIg exposure with outcomes and adjust for confounders. The study included 143 patients, with a 2:1 ratio of controls to patients receiving IVIg treatment. The baseline characteristics were similar between groups. There was higher live birth rate (LBR) in patients receiving IVIg (32/49; 65.3%) compared to controls (32/94; 34%); p < 0.001). When stratifying patients into moderate and severe uRIF (respectively 3–4 and \(\ge\) 5 previous good quality blastocyst transfer failures), only patients with severe uRIF benefited from IVIg (LBR (20/29 (69%) versus 5/25 (20%) for controls, p = 0.0004). In the logistic regression analysis, IVIg was associated with a higher odds of live birth (OR 3.64; 95% CI: 1.78–7.67; p = 0.0004). There were no serious adverse events with IVIg. In conclusion, it is reasonable to consider IVIg in well selected patients with \(\ge\) 5 previous unexplained, high quality blastocyst transfer failures. A well-designed randomized controlled trial is needed to confirm these findings.
RETRACTED: Propitious implementation of Acacia Arabica extract in reducing Candida Albicans adherence’ to different denture bases: In-vitro study
Authors: El-Din Mai Salah , Shahat Michael G , Zayed Sara M 
Published: October 2023

Abstract

The authors have requested the removal of their preprint from Research Square, due to current circumstances that make preprinting challenging at this time. The author's request does not imply errors or misconduct.