bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluid Flow, Mineralization and Deformation in an Oceanic Detachment Fault: Microtextural, Geochemical and Isotopic Evidence From Pyrite at 13°30′N on the Mid‐Atlantic Ridge
Authors: Martin Andrew J. , Jamieson John W. , Petersen Sven , Fayek Mostafa , Escartin Javier 
Published: January 2024

AbstractHydrothermal fluids in ultramafic‐hosted hydrothermal systems associated with oceanic detachment faults can be more oxidizing compared to mafic‐hosted vent sites. These fluids form a mineral assemblage of pyrite, magnetite and hematite. At 13°30′N on the Mid‐Atlantic Ridge, chlorite‐quartz breccias recovered from an exposed fault scarp contain pyrite, with abundant magnetite and hematite, indicating that the redox of the fluids was variable. In primary micron‐scale zonations in pyrite, Ni, Co, and Se have a decoupled relationship, recording fluctuations in the chemical composition and temperature of hydrothermal fluid as the grains grew. Secondary zonations that erase and overprint primary zonations are limited to the grain margin and permeable regions within the grain core. Secondary zonations formed via two processes: (a) grain dissolution followed by overgrowth, and (b) remobilization of metals during oxidizing fluid flow events. In both instances, Ni and Co have been mobilized and concentrated, and are not lost to the hydrothermal fluid. Superimposed on these features is evidence of grain scale deformation related to periods of fault movement along the detachment surface. Sulfur isotope ratios (δ34S) in pyrite systematically decrease from the grain margin to the grain core, indicating that increased amounts of sulfur were derived from thermochemical sulfate reduction of seawater. Thus, pyrite records the evolution of fluid flow and deformation events during exhumation along the detachment surface from ∼1 to 2 km below the seafloor at the base of the lava pile, with temporal fluctuations in fluid redox identified as an important process in controlling Ni and Co enrichment in pyrite.
The Age and Evolution of Lunar Micro Cold Traps at the Scale of Surface Exploration
Authors: Costello E. S. , Lucey P. G. 
Published: January 2024

AbstractCold traps are locations on the Moon that are shielded from sunlight where volatiles such as water could accumulate and persist against sublimation for geologic timescales. We model how long it takes accumulating craters to produce and then obliterate sub‐kilometer scale cold traps. Sub‐meter cold traps are extremely ephemeral, evolving in and out of existence over less than a few thousand years; however, larger 100 m to 1 km‐scale cold traps may persevere for geologic timescales and preserve a record of the volatile history of the Moon.
Elucidating the Multi‐Timescale Variability of a Canopy Urban Heat Island by Using the Short‐Time Fourier Transform
Authors: Zhang Longrui , Luo Fu , Pan Guitao , Zhang Wenjie , Ren Guoyu , Zheng Zuofang , Yang Yuanjian 
Published: January 2024

AbstractTaking the megacity of Beijing as an example, a short‐time Fourier transform (STFT) method was employed to extract the multi‐timescale evolution pattern of the canopy urban heat island intensity (CUHII) during 2000–2020. The STFT of CUHII showed a close relationship between the evolution of the CUHII in Beijing and the background meteorological forcing at intra‐annual, weather and intra‐daily scales. The intra‐annual‐scale spectrum of CUHII exhibited an increasing trend with obvious seasonal variation of the canopy urban heat island (CUHI). The intra‐daily‐scale spectrum of CUHII showed an increasing trend with the nighttime CUHI developing faster. Increasing Western Pacific Subtropical High intensity can enhance the seasonal and diurnal fluctuations of CUHII. The weather‐scale spectrum of CUHII is controlled by weather system evolution, showing that the frequency of cold/heat waves (CWs/HWs) in Beijing was significantly negatively correlated with the weather‐scale spectral intensity of the CUHII. CWs and HWs can increase the CUHII for a long duration.
Recent Opposite Trends of Atmospheric Rivers Over East Asia and Western North Pacific Driven by the Pacific Decadal Oscillation
Authors: Huang Wenshuo , Hua Lijuan , Zhong Linhao , Yang Yang , Gong Zhaohui 
Published: January 2024

AbstractAtmospheric rivers (ARs) in East Asia have garnered increasing attention for their crucial roles in understanding extreme precipitation events and regional hydrological cycles. Here, we investigate the summer AR frequency over the East Asia‐western North Pacific region (EA‐WNP) during 1959–2022. Interestingly, the results show that the AR frequency over the two regions experienced opposite variations, with the boundary between them at around 135°–140°E. On interdecadal timescales, such variations are mainly synchronized with a zonal dipole pattern characterized by cyclonic (anticyclonic) anomalies centered to the northwest of Taiwan and anticyclonic (cyclonic) anomalies centered to the southeast of Japan. This interdecadal dipole pattern is induced by interdecadal changes in the western North Pacific subtropical high under different Pacific Decadal Oscillation (PDO) phases. Particularly, the internal variability accounts for about 77–90% of the observed trends in high response areas. The displacements of ARs are also consistent with the opposite trends of precipitation over EA‐WNP. ARs exert a dominant influence on the precipitation trend in the WNP region but a weaker contribution to the precipitation trend in the EA region, which reflects the different impacts of ARs on EA and WNP precipitation. These results indicate that the PDO has the potential for predicting EA‐WNP ARs on interdecadal timescales, and the opposite AR trends are crucial in understanding the complexity of precipitation over the EA‐WNP region.
An Online Assimilation Method to Improve the Numerical Forecast of Sea Fog Using Microwave Radiometer‐Retrieved Cloud Water Path
Authors: Gao Xiaoyu , Bao Xinghua , Ma Suhong , Chen Qi , Wang Bin 
Published: January 2024

AbstractNumerical forecast of the sea fog is sensitive to the initial moist stratification within the marine atmospheric boundary layer (MABL). This study develops an online assimilation method to improve the MABL thermal and moist structures in sea fog ensemble forecasts based on the Weather Research and Forecasting model and Grid‐point Statistical Interpolation/EnKF system. It uses the satellite‐retrieved cloud water path (CWP) as the indicator of sea fog and low‐level stratus to determine the best ensemble members at each grid point. The relative humidity and cloud water profiles are extracted from the best members to generate a series of pseudo‐observations, which are assimilated to update all the members by EnKF method. The new method significantly improves the ensemble forecast of five widespread advection fog events over the Yellow Sea, which can be attributed to the decrease of both missed and spurious fog areas. The case study shows that assimilating the information of both humidity and cloud water outperforms assimilating either of them, while the impact of directly assimilating CWP observation is insignificant. The analysis increments of cloud water, thermal and moist structures in MABL together contribute to the correction of forecasted sea fog. The generation of pseudo‐observations can use the dynamic compatibility of the model to alleviate the impact of erroneous data in the observation, leading to the low sensitivity of the new method to CWP retrieval error.
The Roles of Bathymetry and Waves in Rip‐Channel Dynamics
Authors: Christensen D. F. , Raubenheimer B. , Elgar S. 
Published: January 2024

AbstractThe behavior and predictability of rip currents (strong, wave‐driven offshore‐directed surfzone currents) have been studied for decades. However, few studies have examined the effects of rip channel morphology on the rip generation or have compared morphodynamic models with observations. Here, simulations conducted with the numerical morphodynamic model MIKE21 reproduce observed trends in flows and bathymetric evolution for two channels dredged across a nearshore sandbar and terrace on an ocean beach near Duck, NC, USA. Channel dimensions, wave conditions, and flows differed between the two cases. In one case, a strong rip current was driven by moderate height, near‐normally incident waves over an approximately 1‐m deep channel with relatively little bathymetric evolution. In the other case, no rip was generated by the large, near‐normally incident waves over the shallower (∼0.5 m) channel, and the channel migrated in the direction of the mean flow and eventually filled in. The model simulated the flow directions, the generation (or not) of rip currents, and the morphological evolution of the channels reasonably well. Model simulations were then conducted for different combinations of the two channel geometries and two wave conditions to examine the relative importance of the waves and morphology to the rip current evolution. The different bathymetries were the dominant factor controlling the flow, whereas both the initial morphology and wave conditions were important for channel evolution. In addition, channel dimensions affected the spatial distribution of rip current forcings and the relative importance of terms.
Effects of Riming on Ice‐Phase Precipitation Growth and Transport Over an Orographic Barrier
Authors: DeLaFrance Andrew , McMurdie Lynn A. , Rowe Angela K. , Conrick Robert 
Published: January 2024

AbstractThe evolution of ice‐phase particles within precipitating clouds depends on the environmental properties of the cloud and on physical characteristics of the particles themselves, which can be modified by airflow over steep terrain. Through employing a unique Lagrangian particle‐based precipitation model, this study investigates the sensitivities in ice‐phase particle growth and transport due to variabilities in riming processes over an orographic barrier. This analysis is applied to two wintertime stratiform cyclones sampled by in situ aircraft over windward slopes during the Olympic Mountains Experiment. For both events, we simulate the ice‐phase particle evolution and trajectory within a two‐dimensional prescribed state representative of median observed cloud properties. Sensitivity simulations were constructed based on observed variabilities in supercooled liquid water (SLW) properties and its vertical extent above the melting level. Perturbations of SLW concentration equivalent to the 85th and 15th percentiles of observed values, which typically amounted to a change of less than 0.05 g m−3, resulted in respective increases or decreases in the ice‐phase contribution to surface precipitation mass by as much as 50% and horizontal particle trajectories differences exceeding 10 km. Similar sensitivities were found in response to varying the vertical extent of SLW above the melting level and to adjustments in mean SLW droplet size. The significant precipitation response to small variations in cloud properties principally arises from changing rates of rime mass accumulation and correspondingly, increases in particle fall speed. Considerations for the numerical representation of the riming process and its complex effects on precipitation are discussed.
Relevance of the endoplasmic reticulum-mitochondria axis in cancer diagnosis and therapy
Authors: An Garam , Park Junho , Song Jisoo , Hong Taeyeon , Song Gwonhwa , Lim Whasun 
Published: January 2024

AbstractDynamic interactions between organelles are responsible for a variety of intercellular functions, and the endoplasmic reticulum (ER)–mitochondrial axis is recognized as a representative interorganelle system. Several studies have confirmed that most proteins in the physically tethered sites between the ER and mitochondria, called mitochondria-associated ER membranes (MAMs), are vital for intracellular physiology. MAM proteins are involved in the regulation of calcium homeostasis, lipid metabolism, and mitochondrial dynamics and are associated with processes related to intracellular stress conditions, such as oxidative stress and unfolded protein responses. Accumulating evidence has shown that, owing to their extensive involvement in cellular homeostasis, alterations in the ER–mitochondrial axis are one of the etiological factors of tumors. An in-depth understanding of MAM proteins and their impact on cell physiology, particularly in cancers, may help elucidate their potential as diagnostic and therapeutic targets for cancers. For example, the modulation of MAM proteins is utilized not only to target diverse intracellular signaling pathways within cancer cells but also to increase the sensitivity of cancer cells to anticancer reagents and regulate immune cell activities. Therefore, the current review summarizes and discusses recent advances in research on the functional roles of MAM proteins and their characteristics in cancers from a diagnostic perspective. Additionally, this review provides insights into diverse therapeutic strategies that target MAM proteins in various cancer types.
Spatial architectures of somatic mutations in normal prostate, benign prostatic hyperplasia and coexisting prostate cancer
Authors: Chae Jeesoo , Jung Seung-Hyun , Choi Eun Ji , Kim Jae Woong , Kim Na Yung , Moon Sung Won , Lee Ji Youl , Chung Yeun-Jun , Lee Sug Hyung 
Published: January 2024

AbstractThis study aimed to identify somatic mutations in nontumor cells (NSMs) in normal prostate and benign prostatic hyperplasia (BPH) and to determine their relatedness to prostate cancer (PCA). From 22 PCA patients, two prostates were sampled for 3-dimensional mapping (50 normal, 46 BPH and 1 PCA samples), and 20 prostates were trio-sampled (two normal or BPH samples and one PCA sample) and analyzed by whole-genome sequencing. Normal and BPH tissues harbored several driver NSMs and copy number alterations (CNAs), including in FOXA1, but the variations exhibited low incidence, rare recurrence, and rare overlap with PCAs. CNAs, structural variants, and mutation signatures were similar between normal and BPH samples, while BPHs harbored a higher mutation burden, shorter telomere length, larger clone size, and more private NSMs than normal prostates. We identified peripheral-zonal dominance and right-side asymmetry in NSMs, but the asymmetry was heterogeneous between samples. In one normal prostate, private oncogenic RAS-signaling NSMs were detected, suggesting convergence in clonal maintenance. Early embryonic mutations exhibited two distinct distributions, characterized as layered and mixed patterns. Our study identified that the BPH genome differed from the normal prostate genome but was still closer to the normal genome than to the PCA genome, suggesting that BPH might be more related to aging or environmental stress than to tumorigenic processes.
Personal care product use patterns in association with phthalate and replacement biomarkers across pregnancy
Authors: Rosen Emma M. , Stevens Danielle R. , Ramos Amanda M. , McNell Erin E. , Wood Mollie E. , Engel Stephanie M. , Keil Alexander P. , Calafat Antonia M. , Botelho Julianne Cook , Sinkovskaya Elena , Przybylska Ann , Saade George , Abuhamad Alfred , Ferguson Kelly K. 
Published: January 2024

Abstract
Background
Humans are exposed to phthalates, a class of non-persistent chemicals, through multiple products, including personal care and cosmetics. Associations between specific phthalates and product use have been inconsistent. However, determining these connections could provide avenues for exposure reduction.

Objective
Examine the association between patterns of personal care product use and associations with phthalate and replacement biomarkers.

Methods
In the Human Placenta and Phthalates Study, 303 women were enrolled in early pregnancy and followed for up to 8 visits across gestation. At each visit, women completed a questionnaire about product use in the prior 24 hours and contributed urine samples, subsequently analyzed for 18 phthalate and replacement metabolites. At early, mid-, and late pregnancy, questionnaire responses were condensed and repeated metabolite concentrations were averaged. Latent class analysis (LCA) was used to determine groups of women with similar use patterns, and weighted associations between group membership and biomarker concentrations were assessed.

Results
LCA sorted women into groups which largely corresponded to: (1) low fragranced product use (16-23% of women); (2) fragranced product and low body wash use (22–26%); 3) fragranced product and low bar soap use (26–51%); and (4) low product use (7–34%). Monoethyl phthalate (MEP) urinary concentrations were 7–10% lower and concentrations of summed di(2-ethylhexyl) terephthalate metabolites were 15–21% lower among women in the “low fragranced product use” group compared to the population mean. Few other consistent associations between group and biomarker concentrations were noted.

Impact statement
Personal care products and cosmetics are a known exposure source for phthalates and potentially represent one of the most accessible intervention targets for exposure reduction. However, in this analysis accounting for concurrent use and fragranced status of products, we did not find any use patterns that corresponded to universally lower levels.
Genomic and functional impact of Trp53 inactivation in JAK2V617F myeloproliferative neoplasms
Authors: Gou Panhong , Liu Duanya , Ganesan Saravanan , Lauret Evelyne , Maslah Nabih , Parietti Veronique , Zhang Wenchao , Meignin Véronique , Kiladjian Jean-Jacques , Cassinat Bruno , Giraudier Stephane 
Published: January 2024

AbstractClassical myeloproliferative neoplasms (MPNs) are characterized by the proliferation of myeloid cells and the risk of transformation into myelofibrosis or acute myeloid leukemia (AML) and TP53 mutations in MPN patients are linked to AML. However, JAK2V617F has been reported to impact the TP53 response to DNA damage, suggesting potential overlapping role of TP53 inactivation in MPN. We established a mouse model showing that JAK2V617F/Vav-Cre/Trp53−/− mice displayed a similar phenotype to JAK2V617F/Vav-Cre mice, but their proliferation was outcompeted in competitive grafts. RNA-Seq revealed that half of the genes affected by JAK2V617F were affected by p53-inactivation, including the interferon pathway. To validate this finding, mice were repopulated with a mixture of wild-type and JAK2V617F (or JAK2V617F/Vav-Cre/Trp53−/−) cells and treated with pegylated interferonα. JAK2V617F-reconstituted mice entered complete hematological remission, while JAK2V617F/Vav-Cre /Trp53−/−-reconstituted mice did not, confirming that p53 loss induced interferon-α resistance. KEGG and Gene Ontology analyses of common deregulated genes showed that these genes were mainly implicated in cytokine response, proliferation, and leukemia evolution, illustrating that in this mouse model, the development of MPN is not affected by TP53 inactivation. Taken together, our results show that many genetic modifications induced by JAK2V617F are influenced by TP53, the MPN phenotype may not be. Trp53 loss alone is insufficient to induce rapid leukemic transformation in steady-state hematopoiesis in JAK2V617F MPN, and Trp53 loss may contribute to interferon resistance in MPN.
Nuclear pore protein POM121 regulates subcellular localization and transcriptional activity of PPARγ
Authors: Yu Yanxiong , Farooq Mohammad S. , Eberhart Meessen Sabine , Jiang Yidan , Kato Dominik , Zhan Tianzuo , Weiss Christel , Seger Rony , Kang Wei , Zhang Xiang , Yu Jun , Ebert Matthias P. A. , Burgermeister Elke 
Published: January 2024

AbstractManipulation of the subcellular localization of transcription factors by preventing their shuttling via the nuclear pore complex (NPC) emerges as a novel therapeutic strategy against cancer. One transmembrane component of the NPC is POM121, encoded by a tandem gene locus POM121A/C on chromosome 7. Overexpression of POM121 is associated with metabolic diseases (e.g., diabetes) and unfavorable clinical outcome in patients with colorectal cancer (CRC). Peroxisome proliferator-activated receptor-gamma (PPARγ) is a transcription factor with anti-diabetic and anti-tumoral efficacy. It is inhibited by export from the nucleus to the cytosol via the RAS-RAF-MEK1/2-ERK1/2 signaling pathway, a major oncogenic driver of CRC. We therefore hypothesized that POM121 participates in the transport of PPARγ across the NPC to regulate its transcriptional activity on genes involved in metabolic and tumor control. We found that POM121A/C mRNA was enriched and POM121 protein co-expressed with PPARγ in tissues from CRC patients conferring poor prognosis. Its interactome was predicted to include proteins responsible for tumor metabolism and immunity, and in-silico modeling provided insights into potential 3D structures of POM121. A peptide region downstream of the nuclear localization sequence (NLS) of POM121 was identified as a cytoplasmic interactor of PPARγ. POM121 positivity correlated with the cytoplasmic localization of PPARγ in patients with KRAS mutant CRC. In contrast, POM121A/C silencing by CRISPR/Cas9 sgRNA or siRNA enforced nuclear accumulation of PPARγ and activated PPARγ target genes promoting lipid metabolism and cell cycle arrest resulting in reduced proliferation of human CRC cells. Our data suggest the POM121-PPARγ axis as a potential drugable target in CRC.
Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells
Authors: Shao Jiawei , Li Shichao , Qiu Xinyuan , Jiang Jian , Zhang Lihang , Wang Pengli , Si Yaqing , Wu Yuhang , He Minghui , Xiong Qiqi , Zhao Liuqi , Li Yilin , Fan Yuxuan , Viviani Mirta , Fu Yu , Wu Chaohua , Gao Ting , Zhu Lingyun , Fussenegger Martin , Wang Hui , Xie Mingqi 
Published: January 2024

AbstractHere, we present a gene regulation strategy enabling programmable control over eukaryotic translational initiation. By excising the natural poly-adenylation (poly-A) signal of target genes and replacing it with a synthetic control region harboring RNA-binding protein (RBP)-specific aptamers, cap-dependent translation is rendered exclusively dependent on synthetic translation initiation factors (STIFs) containing different RBPs engineered to conditionally associate with different eIF4F-binding proteins (eIFBPs). This modular design framework facilitates the engineering of various gene switches and intracellular sensors responding to many user-defined trigger signals of interest, demonstrating tightly controlled, rapid and reversible regulation of transgene expression in mammalian cells as well as compatibility with various clinically applicable delivery routes of in vivo gene therapy. Therapeutic efficacy was demonstrated in two animal models. To exemplify disease treatments that require on-demand drug secretion, we show that a custom-designed gene switch triggered by the FDA-approved drug grazoprevir can effectively control insulin expression and restore glucose homeostasis in diabetic mice. For diseases that require instantaneous sense-and-response treatment programs, we create highly specific sensors for various subcellularly (mis)localized protein markers (such as cancer-related fusion proteins) and show that translation-based protein sensors can be used either alone or in combination with other cell-state classification strategies to create therapeutic biocomputers driving self-sufficient elimination of tumor cells in mice. This design strategy demonstrates unprecedented flexibility for translational regulation and could form the basis for a novel class of programmable gene therapies in vivo.