bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood mononuclear cell molecular landscape associated with tumor progression in triple-negative breast cancer
Authors: Patysheva M. R. , Frolova A. A. , Bragina O. D. , Fedorov A. A. , Vostrikova M. A. , Garbukov E. Yu. , Iamshchikov P. S. , Vashisth M. , Cherdyntseva N. V. , Gerashchenko T. S. 
Published: November 2023

Introduction. triple negative breast cancer is an aggressive clinical phenotype characterized by poor prognosis. immune system plays an important role in the development, treatment response, and progression of solid tumor. The search for immune-related markers associated with the prediction of treatment efficacy and disease prognosis, and based on the use of high-resolution molecular techniques, is a promising area of research, the results of which can be translated into clinical practice. Case description. The molecular profile of blood mononuclear cells in a 48-year-old female patient with histologically proven triple negative breast cancer (estrogen Receptor – 0; progesteron Receptor – 0; Her2/neu – 0; gata-3 – 0, androgen Receptor – 0 and Ki67 – 70 %) was described. The patient did not response to neoadjuvant chemotherapy with 4 cycles of paclitaxel + carboplatin followed by 2 cycles of adriamycin + cyclophosphamide. The patient underwent surgery. disease progression (pelvic bone metastases) occurred 2 months after surgery. The features of blood lymphocytes and monocytes associated with a lack of response to neoadjuvant chemotherapy and disease progression were described.Conclusion. This clinical case demonstrates that sequencing of peripheral blood mononuclear cells can be used as a method for identifying predictive markers of therapy efficacy and developing personalized treatments for patients with triple negative breast cancer.
Endoscopic autofluorescence imaging in the diagnosis of premalignant lesions and laryngeal/hypopharyngeal cancer
Authors: Taraskina I. О. , Cheremisina О. V. , Pankova О. V. , Mukhamedov M. R. , Ananina О. A. 
Published: November 2023

The purposeof the study was to assess the diagnostic value of endoscopic autofluorescence imaging (AFI) in patients with premalignant lesions and primary laryngeal/hypopharyngeal cancer.Material and Methods. The diagnostic value of AFI was assessed in 53 patients with chronic hyperplastic laryngeal/hypopharyngeal lesions and 48 patients with laryngeal/hypopharyngeal cancer.Results. The inclusion of video laryngoscopy with AFI in the algorithm for examining patients with chronic diseases of the upper respiratory tract made it possible to significantly improve the diagnostic efficacy of endoscopic examination in patients with premalignant lesions (high grade dysplasia) of the laryngeal mucosa. Video laryngoscopy with AFI was found to achieve higher sensitivity, specificity and accuracy rates than white light video laryngoscopy (87.5, 96.9 and 92.5 vs 50.0, 96.0 and 71.7 %, respectively, p<0.05). The video laryngoscopy with AFI allowed identification of not only pathological changes in the laryngeal and hypopharyngeal mucosa but also their malignant potential, as well as identification of areas for targeted biopsy. In 9 (18.8 %) laryngeal cancer patients, the standard white light video laryngoscopy demonstrated difficulties in the differential diagnosis of laryngeal cancer (n=4) and in assessing the extent of laryngeal cancer (n=5). In 3 out of 4 diagnostically difficult cases, video laryngoscopy with AFI made it possible to confirm the diagnosis of laryngeal cancer and in 5 cases to accurately determine the extent of hypopharyngeal cancer.Conclusion.The addition of standard videolaryngoscopy with a hightech technique of autofluorescence endoscopy makes it possible to accurately diagnose premalignant and malignant lesions of the larynx and hypopharynx.
Measurement of calcitonin and cea in the needle washout fluid from thyroid nodule for the diagnosis of medullary thyroid carcinoma
Authors: Severskaya N. V. , Chebotareva I. V. , Zhelonkina N. V. , Belyakova A. S. , Isaev P. A. , Polkin V. V. , Ilyin A. A. , Ivanov S. A. , Kaprin A. D. 
Published: November 2023

Background. medullary thyroid carcinoma (mtc) produces serum markers including calcitonin (ct) and carcinoembryonic antigen (cea). The measurement of ct in fine-needle aspirate washout fluid (FNA-CT) improves the cytological diagnosis of mtc. However, no data are available about cut-off values for FNA-CT using currently immunoassay. The measurement of cea in the needle washout fluid (FNa-cea) in mtc has not been studied.Objective: to assess the diagnostic value and propose cut-off values for FNA-CT and FNacea in the thyroid nodule to diagnose mtc.Material and Methods. We conducted a retrospective analysis of 164 samples of fine-needle aspirate washout fluid collected from 92 patients with thyroid nodules, who underwent FNa followed by cytological examination and measurement of FNA-CT. seventeen cases with mtc and 41 with non-mtc nodules were histologically verified. one hundred and six nodules identified as non-mtc by cytology were not operated on. FNa-cea was additionally studied in 29 samples. The cut-off value was determined by Roc analysis.Results. The FNA-CT level was >2000 pg/ml in all mtc nodules, except for one, in which the FNA-CT level was 638 pg/ml. In non-mtc nodules, the FNA-CT levels were <10 pg/ml and <100 pg/ml in 81 % and 90 %, respectively, however, it was >500 pg/ml in 5 %, and >1000 pg/ml in 2 %. At a cut-off value of 590 pg/ml, the sensitivity and specificity of FNA-CT were 100 % and 96 %, and at a cut-off of 1721 pg/ml, the corresponding values were 94 % and 99 %. The false positive FNA-CT values were 3.7 % and 1.2 % at the cut-off values of 590 pg/ml and 1721 pg/ml, respectively. The median levels of FNa-cea in mtc and non-mtc nodules were 59.3 ng/ml and 1.5 ng/ml, respectively. At a cut-off value of 7.5 ng/ml, the sensitivity of FNa-cea was 86 % and specificity was 100 %. Additional measurement of FNa-cea prevented all false-positive results of FNA-CT, but did not detect 2 mtcs with low FNa-cea levels (false-negative rate of 6.9 %). Among samples with FNA-CT value of >590 pg/ml and negative cytology (n=9), FNa-cea differentiated mtc with 100 % sensitivity and specificity.Conclusion. The maximum sensitivity of FNA-CT in the thyroid nodule was at a cut-off value of 590 pg/ml (100 %), the maximum specificity was at a cut-off value of 1721 pg/ml (99 %). Risk of false positive result is the major challenge of FNA-CT. to reduce false-positive results, we recommend to measure FNa-cea in the nodules with negative cytology and high level of FNA-CT. At a cut-off value of 7.5 ng/ml, FNa-cea allows the false-positive FNA-CT to be excluded.
Differential thermal analysis of moisture binding in zephyr with different contents of glucose syrup
Authors: Plotnikova Inessa , Magomedov Gazibeg , Kazartsev Dmitry , Magomedov Magomed , Polansky Konstantin , Plotnikov Viktor 
Published: November 2023

When formulating a product, it is just as important to measure changes in free and bound moisture as it is to analyze quality indicators. Zephyr, a Russian whipped dessert, made with sugar dries quickly during storage, gradually losing its moisture. Its crystalline sugar crust thickens and its entire mass saccharifies, resulting in higher firmness and poor appearance. In this study, we aimed to determine the effect of high-conversion glucose syrup on the amount of moisture and its binding forms in zephyr after storage.
We studied four samples of pectin-based zephyr with different carbohydrate profiles after three months of storage. Differential scanning calorimetry, thermogravimetry, and non-isothermal kinetics were applied to assess moisture contents and forms of binding in zephyr.
Thermograms with thermoanalytical curves were used to analyze the thermolysis of zephyr samples with different contents of glucose syrup in the temperature range from 20 to 300°C. We also studied the endothermic effects at various stages of thermolysis and measured free and bound moisture in the samples. Four stages of their dehydration were identified on the basis of graphical dependences between weight changes and heating temperatures.
The control zephyr sample contained more capillary and polymolecular bound moisture, while the experimental samples in which sugar and confectioner’s syrup were partially or completely replaced with high-conversion glucose syrup had more polymolecular and monomolecular bound moisture. The use of high-conversion glucose syrup instead of sugar and confectioner’s syrup reduced the amount of free moisture and therefore increased the amount of bound moisture, keeping zephyr fresh throughout its shelf life.
Plant proteases and anti-bacterial substances in Allium sativum L. varieties
Authors: Budianto Budianto , Arifin Muh Jaenal , Naryani Naryani , Sukmawati Endah , Suwaji Suwaji , Wibowo Tri Handoko Moedji , Luviana Syahilla Vanda , Putri Lia Dekryt Vergiana 
Published: November 2023

Allium sativum L. protease still remains largely understudied although new varieties of garlic appear quite often, e.g., lanang garlic. This study tested the antibacterial effect of garlic and the effectiveness of various A. sativum proteases as meat tenderizers.
The research involved powder extracts of four varieties of A. sativum: kating, lanang, black garlic, and sin-chung. The degradation kinetics was defined based on the Lineweaver-Burk equation. The degradation zones were measured using sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS-PAGE). Scan electron microscopy served to test the changes in meat connective tissue.
Lanang demonstrated the largest inhibition zones against Escherichia coli (9.75 ± 0.15 mm) and Staphylococcus aureus (1.04 mm). Sin-chung protease degraded beef protein with the highest Vmax of 0.1818 μg/μL/min at 10–22 KDa (small peptide, troponin C, and troponin I), 25–40 KDa (myosin light chain, troponin T, α and β tropomyosin, actin), and 100–140 KDa (protein C). The same garlic variety degraded mutton meat protein at 10–17 KDa (small peptide) and 25–40 KDa (myosin light chain, troponin T, α and β tropomyosin, actin) with Vmax of 0.1135 μg/μL/min.
All four A. sativum proteases proved to be quite effective meat tenderizers.
CHOROIDAL OSTEOMA: REVIEW OF LITERATURE AND FIRST CASE FROM YEMEN
Authors: Al-Shamahi Essam Yahiya , Al-Shamahi Nashwan Yahya A , Al-Shamahi Emad Hassan , Al-Shamahy Hassan Abdulwahab 
Published: November 2023

Juxtapapillary choroidal tumors called choroidal osteomas are benign and do not require medical attention. They predominantly occur in young females (median age, 20 years). Ophthalmoscopy and fluorescein angiography can disclose indicative ocular signs, but computed tomography (CT), echography, and plain radiography are particularly crucial for making the right diagnosis. These lesions may be clinically misdiagnosed as a number of dystrophic lesions, as metastatic carcinoma, atypical malignant choroidal melanoma, metastatic carcinoma, leukemic or lymphoma infiltration, choroidal macular scarring, choroidal hemangiomas, or organized subretinal hemorrhage. As therapeutic methods, radiation and enucleation, it is crucial to rule out malignant melanoma from the differential diagnosis. According to an assessment of the literature, all reported instances with CT scans exhibited calcific densities that could be easily seen in the affected eye's posterior pole. The clinical, radiologic, and pathologic findings in our patient are all typical. We emphasize the importance of CT in assessing choroidal osteoma as well as the uniqueness of CT results.

Peer Review History:
Received: 11 August 2023; Revised: 14 September; Accepted: 28 October, Available online: 15 November 2023
Academic Editor: Dr. Rola Jadallah, Arab American University, Palestine, rola@aauj.edu
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 7.5/10
Reviewers:
Prof. Gorkem Dulger, Duzce University, Turkey, gorkemdulger@yandex.com
Dr. Bilge Ahsen KARA, Ankara Gazi Mustafa Kemal Hospital, Turkey, ahsndkyc@gmail.com
EPIDEMIOLOGICAL ASPECT OF POSTOPERATIVE GASTROINTESTINAL NUTRITIONAL DEFICIENCY IN NGAOUNDERE HOSPITALS, ADAMAWA REGION, CAMEROON
Authors: Raoul Sineche Ngunte , Valentine Tsague Marthe , Marcel Nguimbou Richard , Roger Ngadjui Ngodjoum Donald , Archange Modjo Gabriel , Jacqueline Ze Minkande 
Published: November 2023

Background and objective: Undernutrition is defined as an insufficient intake of nutritional foods. The aim of this study was to evaluate the epidemiological aspect of postoperative gastrointestinal nutritional deficiency in Ngaoundere Hospitals.
Methods: It was a prospective, cross-sectional study with an analytical focus, over a 5-month period from July to November 2022. Undernutrition was defined by the presence of at least one phenotypic criteria plus one etiological criteria. Logistic regression for multivariate analyses, and the Chi-square test for univariate analyses, were used to identify the determinants of undernutrition. A difference was considered significant if p<0.05.
Results: A total of 134 patients were included in the current study. Males were the most represented, with rates of 82.09%. The average age of patients was 36±17.78. Undernutrition at discharge was observed in 110 patients (82.09%). Endogenous risk factors associated with the occurrence of undernutrition were: Muslim religion, persistent symptoms, emergency surgery modality, nutritional grades, postoperative nausea, and diarrhea (p<0.0001); the protective one was minor surgery (p<0.0001). Exogenous risk factors for undernutrition included failure to meet carbohydrate, protein, and calorie targets on day-3 and at discharge (p<0.0001). Finally, in multivariate analysis, persistent symptoms (p=0.02), minor surgery (p=0.04), presence of diarrhea (p=0.01), and failure to meet caloric targets were independently associated with undernutrition at discharge.
Conclusion: The incidence of post-digestive surgery undernutrition is relatively high in the 2 hospitals in Adamawa, Cameroon. It, therefore, requires early detection and management.

Peer Review History:
Received: 7 September 2023; Revised: 2 October; Accepted: 9 November, Available online: 15 November 2023
Academic Editor: Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 7.0/10
Reviewers:
Prof. Hassan A.H. Al-Shamahy, Sana'a University, Yemen, shmahe@yemen.net.ye
Dr. Owanaro Peter, Nigeria, Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria. owonaropeter@gmail.com
ANTIBIOTIC SENSITIVITY OF BACTERIAL BLOODSTREAM INFECTIONS IN THE INTENSIVE CARE UNIT PATIENTS OF UNIVERSITY HOSPITALS IN SANA'A CITY, YEMEN
Authors: Al-Yosaffi Eshtiaq A , Al-Shamahy Hassan Abdulwahab , Othman Arwa Mohammed , Al-Haddad Ahmed Mohammed , Al-Moyed Khaled Abdulkarim 
Published: November 2023

Aim: High rates of morbidity and mortality are associated to bacterial bloodstream infections (B-BSI) in many hospitals, especially in the intensive care unit. This study investigated the prevalence of antibiotic- and multidrug-resistant bacteria isolated from blood samples of patients in intensive care units of university hospitals in the city of Sana'a, Yemen.
Subjects and methods: A cross-sectional study was conducted on sepsis patients admitted to intensive care units in four hospitals in Sana'a, Yemen, between January 1 and April 30, 2022. The blood cultures of patients suspected of suffering from sepsis were performed. The potential bacterial pathogens were isolated and identified using standard laboratory methods, and microbial susceptibility testing was performed using the disk diffusion technique.
Results: For all identified bacteria, the average resistance rate to a broad spectrum of antibiotics tested ranged from 22.5% to 98.1%, with cefazoline (98.1%) having the greatest resistance rates, followed by amoxicillin (87.2%) and cefixime (83%). Vancomycin had a resistance rate of 4.8% whereas erythromycin had a resistance rate of 75% for Gram-positive bacteria. For Gram-negative bacteria, the resistance rates to narrow spectrum antibiotics ranged from 2.3% for colistin sulphate to 84.8% for aztreonam. Our isolates' MDR rates for resistance to at least three classes of antibiotics were 52.2% and 8.7%, respectively, for resistance to 10 different classes of broad-spectrum antibiotics and their subclasses.
Conclusion: Gram positive bacteria are highly resistant to erythromycin and penicillin, while gram negative organisms are highly resistant to amoxcillin+clavulanic acid, ciprofloxacin, and all generations of cephalosporins. This study highlights the significance of prompt clinical and bacteriological monitoring among patients in critical care conditions, such as ICU patients, and also illustrates the establishment and rates of Multi Drug Resistance (MDR) pathogens.

Peer Review History:
Received: 27 August 2023; Revised: 13 September; Accepted: 29 October, Available online: 15 November 2023
Academic Editor: Dr. Asia Selman Abdullah, Pharmacy institute, University of Basrah, Iraq, asia_abdullah65@yahoo.com
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 7.5/10
Reviewers:
Dr. Tamer Elhabibi, Suez Canal University, Egypt, tamer_hassan@pharm.suez.edu.eg
Dr. Wadhah Hassan Ali Edrees, Hajja University, Yemen, edress2020@gmail.com
DETERMINATION OF MINERALS IN LAVANDULA STOECHAS L. SUBSP. CARIENSIS (BOISS.) ROZEIRA BY X-RAY FLUORESCENCE SPECTROSCOPY
Authors: Hüsniye Kayalar , Sinem Yılmaz Zehra , Cenk Durmuşkahya , Ümit Toktaş 
Published: November 2023

Background and objective: Medicinal herbs are sources of bioactive plant secondary metabolites such as phenolic acids, flavonoids, iridoids, tannins, anthracenes, alkaloids, terpenes. Herbs and herbal extracts are important sources for minerals that can create health effects in human diet. The infusion and decoction prepared from Lavandula stoechas L., subsp. cariensis known as Karabaşotu in Turkey, are used in traditional medicine. The objective of the present work was to comparatively determine the mineral content of dried aerial parts L. stoeachas L. subsp. cariensis and its water extract which is prepared by 2% infusion.
Methods: The aerial parts of Lavandula stoechas L., were air-dried and grounded. 2% infusion was prepared by distilled water and water was evaporated until dryness. The water extract and the plant powder were analyzed for heavy metals and trace elements by Spectro-IQ II instrument.
Results: The analyzed samples differed significantly in terms of mineral contents. The powdered aerial parts of L. stoechas L. subsp. cariensis and its water extract are characterized by high contents of potassium, calcium, sodium, chlorine, phosphorous and magnesium. Iron in powdered plant material (576.6 ppm) was found to be almost ten times higher than the water extract (51.7 ppm). Microelements such as copper (78.8 ppm) and zinc (3169 ppm) accumulated at higher levels in the water extract of L. stoechas L. subsp. cariensis.
Conclusion: The mineral content of the water extract and the powdered aerial parts of L. stoechas L. subsp. cariensis are quantitatively determined by X-ray fluorescence spectroscopy for the first time. L. stoechas L. subsp. Cariensis collected from other regions of Turkey will be comparatively analyzed by XRF spectrometry in our further studies.

Peer Review History:
Received: 23 August 2023; Revised: 26 September; Accepted: 30 October, Available online: 15 November 2023
Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt, gehankandeel9@yahoo.com
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 7.0/10
Reviewers:
Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my
Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, ahmad.najib@umi.ac.id
RADIOGRAPHIC EVALUATION OF OVERHANGING RESTORATIONS AMONG YEMENI PATIENTS USING CONE-BEAM COMPUTED TOMOGRAPHY (CBCT)
Authors: Alsada'a Wahip Ahmed Ali , Al-Hamzi Mohsen Ali , Al-Khawlani Abdulwahab Ismail , Al-Shamahy Hassan Abdulwahab 
Published: November 2023

Background and aims: Overhanging restorations which is extension of restorative material beyond the confines of a cavity preparation may lead to various complications and risks, including increased gingival fluid flow and oral inflammation. The study aim is to use cone-beam computed tomography (CBCT) to radiographically assess the incidence of overhanging restorations in Yemeni patients.
Methods: A retrospective comparative study was conducted on 404 randomly selected CBCT images dor ≥ 18 years male and female Yemeni patients referred to private radiology centers in different Yemeni cities (i.e., Sana'a, Ibb, Hodeida, Aden and Hadhramout) during the period from January 2021 to June 2022. The overhang was evaluated regarding arch (upper or lower), side (right or left), tooth location, and tooth type (central incisor (I1), lateral incisor (I2), canine (C), 1st premolar (P1), 2nd premolar (P2), 1st molar (M1), 2nd molar (M2) and 3rd molar (M3). The tooth surface was considered the statistical unit to allow an accurate comparison of periodontal variables for each restored surface. The evaluated surface points of class II restorations were mesial and distal, while those of crown restorations were mesial, distal, buccal, lingual, mesiobuccal, mesiolingual, distobuccal, and distolingual.
Results: The prevalence rate of overhang was 59.8% in class II and 51.5% in crown restorations. Class II restoration overhang had no significant association with arch, side, tooth type, and tooth location; however, it had a significant association with tooth and surface point (p<0.05), indicating that it was more significantly observed in the 2nd premolar (67.5%) than the other teeth as well as in the distal surface than the mesial one (73.1% vs. 26.9%, p<0.05
Conclusion: The prevalence of class II and crown restoration overhang is considered high among Yemeni patients. Overhangs observed on class II restorations are more than those observed on crown restorations. Class II restoration overhangs are more commonly observed on the 2nd premolar as well as the distal surface.

Peer Review History:
Received: 8 August 2023; Revised: 19 September; Accepted: 26 October, Available online: 15 November 2023
Academic Editor: Dr. DANIYAN Oluwatoyin Michael, Obafemi Awolowo University, ILE-IFE, Nigeria, toyinpharm@gmail.com
Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 8.0/10
Reviewers:
Dr. Tamer Elhabibi, Suez Canal University, Egypt, tamer_hassan@pharm.suez.edu.eg
Dr. Bilge Ahsen KARA, Ankara Gazi Mustafa Kemal Hospital, Turkey, ahsndkyc@gmail.com