bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ex-vivo CS1-OKT3 dual specific bivalent antibody-armed effector T cells mediate cellular immunity against multiple myeloma
Authors: Awuah Dennis , Li Lin , Williams Lindsay , Urak Ryan , Kujawski Maciej , Forman Stephen J. , Shively John E. , Wang Xiuli 
Published: November 2023

AbstractBispecific T cell engaging antibodies (bsAbs) have emerged as novel and powerful therapeutic agents for redirecting T cells towards antigen-specific tumor killing. The cell surface glycoprotein and SLAM family member, CS1, exhibits stable and high-level expression on malignant plasma cells including multiple myeloma, which is indicative of an ideal target for bsAb therapy. Here, we developed a CS1 bsAb (CS1-dbBiTE) using Click chemistry to conjugate intact anti-CS1 antibody (Elotuzumab) and anti-huOKT3 antibody at their respective hinge regions. Using a cellular therapy approach, human T cells were armed ex-vivo with CS1-dbBiTE prior to examining effector activity. Our data indicates that arming T cells with CS1-dbBiTE induced T cell activation and expansion and subsequent cytotoxic activity against CS1-bearing MM tumors, demonstrated by significant CD107a expression as well as inflammatory cytokine secretion. As expected, CS1-dbBiTE armed T cells showed significantly reduced effector activity in the absence of CS1 expression. Similarly, in MM mouse xenograft studies, armed T cells exhibited effective anti-tumor efficacy highlighted by reduced tumor burden in MM.1S tumor-bearing mice compared to controls. On the basis of these findings, the rationale for CS1 targeting by human T cells armed with CS1-dbBiTE presents a potentially effective therapeutic approach for targeting MM.
Response surface optimization of process parameters for preparation of cellulose nanocrystal stabilized nanosulphur suspension
Authors: Mahawar Manoj Kumar , Bharimalla Ashok Kumar , Arputharaj A. , Palkar Jagdish , Dhakane-Lad Jyoti , Jalgaonkar Kirti , Vigneshwaran N. 
Published: November 2023

AbstractThis study employed response surface methodology (RSM) to optimize various parameters involved in the synthesis of nanosulphur (NS) stabilized by cellulose nanocrystals (CNCs). The elemental sulphur (ES) mixed with CNCs was processed in a high-pressure homogenizer to make a stable formulation of CNC-stabilized NS (CNC-NS). RSM was adopted to formulate the experiments using Box-Behnken design (BBD) by considering three independent variables i.e., ES (5, 10, 15 g), CNCs (25, 50, 75 ml), and the number of passes (NP) in the high-pressure homogenizer (1, 2, 3). For the prepared suspensions (CNC-NS), the range of the responses viz. settling time (0.84–20.60 min), particle size (500.41–1432.62 nm), viscosity (29.20–420.60 cP), and surface tension (60.35–73.61 N/m) were observed. The numerical optimization technique was followed by keeping the independent and dependent factors in the range yielded in the optimized solution viz. 46 ml (CNCs), 8 g (ES), and 2 (NP). It was interpreted from the findings that the stability of the suspension had a positive correlation with the amount of CNC while the increasing proportion of ES resulted in reduced stability. The quadratic model was fitted adequately to all the responses as justified with the higher coefficient of determination (R2 ≥ 0.88). The characterization performed by X-ray diffraction (XRD), zeta potential, Raman spectroscopy, and Fourier transform infrared spectroscopy (FTIR) revealed better-stabilizing properties of the optimized CNCs–ES suspension. The study confirmed that CNCs have the potential to be utilized as a stabilizing agent in synthesizing stable nanosulphur formulation by high-pressure homogenization.
Active production and passive observation of hand movements shift visual hand location
Authors: Langenberg Maryvonne , Bayer Manuel , Zimmermann Eckart 
Published: November 2023

AbstractWhich factors influence the perception of our hand location is a matter of current debate. Here, we test if sensorimotor processing contributes to the perception of hand location. We developed a novel visuomotor adaptation procedure to measure whether actively performing hand movements or passively observing them, influences visual perception of hand location. Participants had to point with a handheld controller to a briefly presented visual target. When they reached the remembered position of the target, the controller presented a tactile buzz. In adaptation trials, the tactile buzz was presented when the hand had not yet reached the target. Over the course of trials, participants adapted to the manipulation and pointed to a location between the visual target and the tactile buzz. We measured the perceived location of the hand by flashing a virtual pair of left and right hands before and after adaptation. Participants had to judge which hand they perceived closer to their body on the fronto-parallel plane. After adaptation, they judged the right hand, that corresponded to the hand used during adaptation, to be located further away from the body. We conclude that sensorimotor prediction of the consequences of hand movements shape sensory processing of hand location.
A web-based genome-wide association study reveals the susceptibility loci of common adverse events following COVID-19 vaccination in the Japanese population
Authors: Nogawa Shun , Kanamori Hajime , Tokuda Koichi , Kawafune Kaoru , Chijiiwa Miyuki , Saito Kenji , Takahashi Shoko 
Published: November 2023

AbstractThe coronavirus disease 2019 (COVID-19) pandemic has spread rapidly worldwide. To prevent its spread, mRNA-based vaccines made by Pfizer/BioNTech (BNT162b1) and Moderna (mRNA-1273) have been widely used, including in Japan. Various adverse events have been reported following the COVID-19 mRNA vaccination, with differences observed among individuals. However, analyses of the genetic background associated with the susceptibility to side effects have been limited. In the present study, we performed genome-wide association studies (GWAS) for self-reported adverse events of the COVID-19 mRNA vaccination in 4545 Japanese individuals and identified 14 associated loci. Among these, 6p21 was associated with 37.5 °C or higher fever, 38 °C or higher fever, and muscle pain. HLA allele association analysis revealed that various HLA alleles were associated with the adverse effects; HLA-DQA1*03:01 and HLA-A*11:01 were more reliably associated with the adverse effects. Our results may enable the preparation and management of adverse effects by identifying the susceptibility to these adverse events. Furthermore, we obtained valuable data that may lead to a better understanding of the mechanisms of action of the COVID-19 mRNA vaccines.
The role of multimodal cues in second language comprehension
Authors: Zhang Ye , Ding Rong , Frassinelli Diego , Tuomainen Jyrki , Klavinskis-Whiting Sebastian , Vigliocco Gabriella 
Published: November 2023

AbstractIn face-to-face communication, multimodal cues such as prosody, gestures, and mouth movements can play a crucial role in language processing. While several studies have addressed how these cues contribute to native (L1) language processing, their impact on non-native (L2) comprehension is largely unknown. Comprehension of naturalistic language by L2 comprehenders may be supported by the presence of (at least some) multimodal cues, as these provide correlated and convergent information that may aid linguistic processing. However, it is also the case that multimodal cues may be less used by L2 comprehenders because linguistic processing is more demanding than for L1 comprehenders, leaving more limited resources for the processing of multimodal cues. In this study, we investigated how L2 comprehenders use multimodal cues in naturalistic stimuli (while participants watched videos of a speaker), as measured by electrophysiological responses (N400) to words, and whether there are differences between L1 and L2 comprehenders. We found that prosody, gestures, and informative mouth movements each reduced the N400 in L2, indexing easier comprehension. Nevertheless, L2 participants showed weaker effects for each cue compared to L1 comprehenders, with the exception of meaningful gestures and informative mouth movements. These results show that L2 comprehenders focus on specific multimodal cues – meaningful gestures that support meaningful interpretation and mouth movements that enhance the acoustic signal – while using multimodal cues to a lesser extent than L1 comprehenders overall.
Gut microbial signatures are associated with Lynch syndrome (LS) and cancer history in Druze communities in Israel
Authors: Naddaf Rawi , Carasso Shaqed , Reznick-Levi Gili , Hasnis Erez , Qarawani Amalfi , Maza Itay , Gefen Tal , Half Elizabeth Emily , Geva-Zatorsky Naama 
Published: November 2023

AbstractLynch syndrome (LS) is a hereditary cancer syndrome caused by autosomal dominant mutations, with high probability of early onset for several cancers, mainly colorectal cancer (CRC). The gut microbiome was shown to be influenced by host genetics and to be altered during cancer development. Therefore, we aimed to determine alterations in gut microbiome compositions of LS patients with and without cancer. We performed fecal microbiome analyses on samples of LS and non-LS members from the Druze ethnoreligious community in Israel, based on both their LS mutation and their cancer history. Our analysis revealed specific bacterial operational taxonomic units (OTUs) overrepresented in LS individuals as well as bacterial OTUs differentiating between the LS individuals with a history of cancer. The identified OTUs align with previous studies either correlating them to pro-inflammatory functions, which can predispose to cancer, or to the cancer itself, and as such, these bacteria can be considered as future therapeutic targets.
Food co-consumption network as a new approach to dietary pattern in non-alcoholic fatty liver disease
Authors: Naghizadeh Mohammad Mehdi , Osati Saeed , Homayounfar Reza , Masoudi-Nejad Ali 
Published: November 2023

AbstractDietary patterns strongly correlate with non-alcoholic fatty liver disease (NAFLD), which is a leading cause of chronic liver disease in developed societies. In this study, we introduce a new definition, the co-consumption network (CCN), which depicts the common consumption patterns of food groups through network analysis. We then examine the relationship between dietary patterns and NAFLD by analyzing this network. We selected 1500 individuals living in Tehran, Iran, cross-sectionally. They completed a food frequency questionnaire and underwent scanning via the FibroScan for liver stiffness, using the CAP score. The food items were categorized into 40 food groups. We reconstructed the CCN using the Spearman correlation-based connection. We then created healthy and unhealthy clusters using the label propagation algorithm. Participants were assigned to two clusters using the hypergeometric distribution. Finally, we classified participants into two healthy NAFLD networks, and reconstructed the gender and disease differential CCNs. We found that the sweet food group was the hub of the proposed CCN, with the largest cliques of size 5 associated with the unhealthy cluster. The unhealthy module members had a significantly higher CAP score (253.7 ± 47.8) compared to the healthy module members (218.0 ± 46.4) (P < 0.001). The disease differential CCN showed that in the case of NAFLD, processed meat had been co-consumed with mayonnaise and soft drinks, in contrast to the healthy participants, who had co-consumed fruits with green leafy and yellow vegetables. The CCN is a powerful method for presenting food groups, their consumption quantity, and their interactions efficiently. Moreover, it facilitates the examination of the relationship between dietary patterns and NAFLD.
Prognostic significance of high NPC2 expression in gastric cancer
Authors: Yao Yunzhuang , Ren Jinnan , Lu Junhui , Sui Yue , Gong Jingwen , Chen Xing 
Published: November 2023

AbstractGastric cancer is one of the most common malignancies worldwide, and the third leading cause of cancer-related death. The identification of novel biomarkers and therapeutic targets is critical to improve the prognosis. A total of 380 patients with primary gastric cancer from the TCGA database were analyzed. The receiver operating characteristic curves were plotted. We further evaluated the independent prognostic ability of NPC2 expression for overall survival (OS) and relapse-free survival (RFS) through the Kaplan–Meier curve and Cox analysis. The NPC2 expression was significantly higher (P < 0.001) in gastric cancer. High NPC2 expression was significantly (P < 0.0001) associated with poor OS and poor RFS. The age, stage, radiation therapy, residual tumor, and NPC2 expression showed independent prognostic value for OS. The gender and NPC2 expression showed independent prognostic value for RFS. The higher NPC2 expression was observed in gastric cancer, compared with adjacent normal tissue (P < 0.001), confirmed by the IHC staining. The CCK-8 assay showed that NPC2 knockdown inhibits cell proliferation while NPC2 overexpression promotes cell proliferation (P < 0.05). NPC2 expression may serve as a promising prognostic biomarker for patients with gastric cancer.
Analysis on the geographical pattern and driving force of traditional villages based on GIS and Geodetector: a case study of Guizhou, China
Authors: Wu Kehua , Su Weici , Ye Shi’an , Li Wei , Cao Yang , Jia Zhenzhen 
Published: November 2023

AbstractTraditional villages have received widespread attention from all walks of life based on important carriers of Chinese rural culture. The mutual superposition of natural and cultural factors may exacerbate the evolution of traditional village geographical patterns. To understand such relationships and effects, factors and degrees influencing traditional villages need to be determined. Here, we analysed the data of 724 traditional villages in Guizhou recognised by relevant national ministries and commissions in China using average nearest neighbour analysis, Tyson polygon analysis, nuclear density analysis and Geodector. The geographic pattern feature revealed that traditional villages, in general, are highly clustered regionally and have significant edge effects on administrative units. Different substrate environments result in significant spatial heterogeneity in village spatial density, clustering, surface undulation, sun exposure, and waterfront. The geographic pattern of traditional villages is mostly affected by the closest distance to river valleys, the types and number of intangible cultural heritage resources in the county, river gorge density, edge effect index, degree of county ethnic language use, and proportion of paddy fields to the regional area; and their combined effects influence and control the community structure. The results highlight the impact of nature and culture on the distribution of traditional villages, which helps traditional village conservation and scientific exploration of human-land relationship issues in the mountainous areas of Southwest China.
Male Pagurus minutus hermit crabs use multiple types of information in decisions to give up male–male contests
Authors: Yasuda Chiaki I. , Koga Tsunenori 
Published: November 2023

AbstractOrganisms use information to make adaptive decisions in various contexts, including aggression. Potentially weaker, but better-informed, contestants should give up earlier to reduce fighting costs by using information related to their own lower success such as their size relative to their opponent and past contest outcomes to make this choice. Here, we examined whether intruders of the hermit crabPagurus minutuscould use information about their (1) smaller size, (2) past contest defeats, (3) opponent’s past wins, or (4) relationship in the dominance hierarchy to their opponent when making a decision to give up during male–male contests for a female. In all trials, we randomly matched a smaller intruder with a larger opponent that was guarding a female. Our analyses suggest thatP. minutusintruders can use all four types of information to decide whether to give up a contest without escalation or decrease its duration after escalation; it is the first species ofPagurusreported to do so, and the second reported to be able to distinguish familiar opponents from others in the context of male–male contests. These findings demonstrate the importance of cognitive abilities in minimizing costs when competing for vital resources.
Towards reliable IoT communication and robust security: investigating trusted schemes in the internet of medical things using blockchain
Authors: Rathee Geetanjali , Maheswar Rajagopal , Sehar Sountharrajan , Bavirisetti Durga Prasad 
Published: November 2023

AbstractThe Internet of Things (IoT) is evolving in various sectors such as industries, healthcare, smart homes, and societies. Billions and trillions of IoT devices are used in e-health systems, known as the Internet of Medical Things (IoMT), to improve communication processes in the network. Scientists and researchers have proposed various methods and schemes to ensure automatic monitoring, communication, diagnosis, and even operating on patients at a distance. Several researchers have proposed security schemes and approaches to identify the legitimacy of intelligent systems involved in maintaining records in the network. However, existing schemes have their own performance issues, including delay, storage efficiency, costs, and others. This paper proposes trusted schemes that combine mean and subjective logic aggregation methods to compute the trust of each communicating device in the network. Additionally, the network maintains a blockchain of legitimate devices to oversee the trusted devices in the network. The proposed mechanism is further verified and analyzed using various security metrics, such as reliability, trust, delay, beliefs, and disbeliefs, in comparison to existing schemes.
Fast track to stroke unit for patients not eligible for acute intervention, a case–control register study on 1066 patients
Authors: Wennman Ingela , Wijk Helle , Jood Katarina , Carlström Eric , Fridlund Bengt , Alsholm Linda , Herlitz Johan , Hansson Per-Olof 
Published: November 2023

AbstractStroke patients not eligible for acute intervention often have low priority and may spend long time at the emergency department (ED) waiting for admission. The aim of this retrospective case–control register study was to evaluate outcomes for such “low priority” stroke patients who were transported via Fast Track directly to the stroke unit, according to pre-specified criteria by emergency medical service (EMS). The outcomes of Fast Track patients, transported directly to stroke unit (cases) were compared with the outcomes of patients who fulfilled these critera for Fast Track, but instead were transported to the ED (controls). In all, 557 cases and 509 controls were identified. The latter spent a mean time of 237 min in the ED before admission. The 90-day mortality rate was 12.9% for cases and 14.7% for controls (n.s.). None of the secondary outcome events differed significantly between the groups: 28-day mortality rate; death rate during hospitalisation; proportion of pneumonias, falls or pressure ulcers; or health-related outcomes according to the EQ-5D-5L questionnaire. These findings indicates that the Fast Track to the stroke unit by an EMS is safe for selected stroke patients and could avoid non-valuable time in the ED.
The characteristic analysis of phase-controlled array thermo-acoustic emission with multiple emitting surfaces
Authors: Zhang Kai , Wang Dongdong , Zhou Jiayi , Su Yulei , Ding Huaikuang , Hu Hanping , Han Yuanzhao 
Published: November 2023

AbstractThermo-acoustic (TA) ultrasound, particularly when combined with phased-controlled array technology, has garnered significant interest in the past decade due to its numerous advantages. This paper establishes a theoretical expression for thermo-acoustic phased array (TAPA) emission to investigate different acoustic fields based on input heat flow frequencies, quantities and distances of TA emission surfaces, area of emission surfaces, and phase changes between emission surfaces. The study finds that a TAPA with two emitting surfaces in a line pattern produces a consistent acoustic field compared to a single emitting surface arranged in a semicircle. Additionally, applying different phases on the surfaces narrows the scanning range with an increase in frequency, area of the TA emission surface, and the amount of emission surfaces, while enhancing the directivity of the TA wave. Moreover, increasing the distance between emitting surfaces in a square-shaped TAPA does not affect the ultrasound pressure of the main TA ultrasound but increases the quantity and size of side lobes. Furthermore, enlarging the area of emitting surfaces enhances the directivity of the TA ultrasound, necessitating a reduction in the distance between emitting surfaces or an increase in the area of the emitting surfaces in a square-shaped TAPA to enhance directivity. This paper provides a comprehensive study of TAPA to aid further research in this field.
Toward steering the motion of surface rolling molecular machines by straining graphene substrate
Authors: Vaezi Mehran , Nejat Pishkenari Hossein 
Published: November 2023

AbstractThe surface rolling molecular machines are proposed to perform tasks and carrying molecular payloads on the substrates. As a result, controlling the surface motion of these molecular machines is of interest for the design of nano-transportation systems. In this study, we evaluate the motion of the nanocar on the graphene nanoribbons with strain gradient, through the molecular dynamics (MD) simulations, and theoretical relations. The nanocar indicates directed motion from the maximum strained part of the graphene to the unstrained end of the substrate. The strain gradient induced driving force and diffusion coefficients of nanocars are analyzed from the simulation and theoretical points of view. To obtain the optimum directed motion of nanocar, we consider the effects of temperature, strain average, and magnitude of strain gradient on the directionality of the motion. Moreover, the mechanism of the motion of nanocar is studied by evaluating the direction of the nanocar’s chassis and the rotation of wheels around the axles. Ultimately, the programmable motion of nanocar is shown by adjusting the strain gradient of graphene substrate.
Modeling walking accessibility to urban parks using Google Maps crowdsourcing database in the high-density urban environments of Hong Kong
Authors: Gong Fang-Ying 
Published: November 2023

AbstractAccessing urban parks is important for promoting physical activities and improving public health. In this study, we propose the use of Google Maps crowdsourcing data and the incorporation of park attractiveness to model urban park accessibility in the complex urban environments of Hong Kong. The difference between using geometric and route distance, the effect of park attractiveness in measuring accessibility, and the benefits gained from using walk time compared to distance are investigated. Our result shows that (1) route and geometric distances have a strong correlation with a conversion factor of about 1.5; (2) the common assumption that park size can be a proxy for describing attractiveness may not be correct. Instead, park attractiveness should be explicitly considered for a more effective accessibility modeling; and (3) estimation by walking time shows that there are non-negligible impacts from street conditions and traffic on urban park accessibility. Moreover, district hotspots short of park accessibility or attractiveness can be explicitly detected. Overall, this developed approach provides a flexible and informative approach to model the accessibility to urban parks. The outputs will help city planners, health professionals, and policymakers to evaluate and improve urban park planning and equity in accessibility in high-density cities.