bluuebunny/crossref_metadata_2025_split huggingface dataset:
Within‐family associations of parent–adolescent relationship quality and adolescent affective well‐being
Authors: Arslan İldeniz B. , Boele Savannah , Dietvorst Evelien , Lucassen Nicole , Keijsers Loes 
Published: February 2024

AbstractBackgroundParent–adolescent relationship quality is theorized to be an important correlate of adolescent affective well‐being. Little is known about the within‐family processes underlying parent–adolescent relationship quality and affective well‐being over a period of months. This three‐wave, preregistered study examined within‐ and between‐family associations between parent–adolescent relationship quality (support and conflict) and adolescent well‐being (negative and positive affect). In addition, we examined whether the associations differed between mothers and fathers, and for adolescents' affective well‐being in different social contexts (at home, at school, with peers).MethodsThe sample consisted of 244 Dutch adolescents (61.5% girls; age range: 12–17 years; mean age = 13.8 years). Random‐intercept cross‐lagged panel models were used.ResultsAt the between‐family level, higher levels of support and lower levels of conflict were associated with higher levels of positive affect and lower levels of negative affect. At the within‐family level, increases in support and decreases in conflict were concurrently associated with increases in positive affect and decreases in negative affect. More parent–adolescent conflict than typical also predicted increases in negative affect, 3 months later, and more negative affect and less positive affect than typical predicted increased conflict, 3 months later. These within‐family effects were largely similar for fathers and mothers. Associations for conflict occurred through bidirectional processes: Parent–adolescent conflict shaped and was shaped by adolescents' emotions at home, at school, and with peers.ConclusionResults suggest that parent–adolescent relationship quality (especially conflict) and adolescent affective well‐being cofluctuate and predict each other over time within families.
Variable‐volume accelerated system to evaluate the carbaryl biodegradation dynamics of a microbial community by differential analysis
Authors: Pérez‐Suárez Cristian , Ruiz‐Ordaz Nora , Galíndez‐Mayer Juvencio , Santoyo‐Tepole Fortunata , Juárez‐Ramírez Cleotilde 
Published: February 2024

AbstractBACKGROUNDOne of the continuous cultivation methods for acquiring quantitative data on microbial metabolism under various environmental conditions within a single experiment is the accelerostat culture system (A‐stat). It allows the rapid analysis and quantitative evaluation of the effect of environmental on the functional response of microorganisms, which is reflected by the cultural biokinetic parameters. In a classical accelerostat system, equipment and control programs maintain a constant acceleration (a) of the culture's dilution rate D[t] = Do + at. Unlike the conventional A‐stat, in which a linear change in the dilution rate is obtained, a new variant, here named a variable‐volume accelerated (VVA) system, the acceleration is variable (a = f[t]); thus, D[t] = Do + a[t]. This system requires minimal manual intervention and presents operational advantages over the classical A‐stat; the reactor's D[t] change is achieved under a simple condition: the input and output flow rates are unequal; Fin ≠ Fout.RESULTSUsing carbaryl as a study model, the dynamic behavior of the VVA system was evaluated by differential analysis of the experimental data obtained by cultivating a carbaryl‐degrading microbial community. Mathematical analysis of the VVA system established the acceleration profile a[t] for the study, quantifying the impact of dilution rate on the microbial community's biokinetic and stoichiometric parameters.CONCLUSIONThe VVA system allowed observation of the effect of the D[t] gradient on the rate of change of kinetic parameters that define the physiological state of the microbial culture, together with the microbial community's macromolecular composition. © 2024 Society of Chemical Industry (SCI).
Assessment of the students’ performance and support needs in a novel digital carving exercise
Authors: Maragha Tala , Chehroudi Babak 
Published: February 2024

AbstractObjectivesIn recent years, digital technology has been rapidly expanding in dental practice, which entails an early integration of digital dentistry into the preclinical dental curriculum. This study introduces first‐year dental students to a digital carving exercise and investigates its role in enhancing their understanding and performance in traditional wax carving activities. Another objective was to explore the students’ challenges and needs for support in the digital carving activity.MethodsDigital carving exercise was introduced into the first‐year dental morphology curriculum in 2020. Students’ performance in anterior wax carving was quantitively compared prior to and following the implementation of the exercise. The students’ grades in the digital carving exercise were also compared across three academic years: 2020, 2021, and 2022. Qualitatively, an interpretive description approach using focus group with 31 first‐year dental students was utilized to explore their perspectives regarding the digital exercise.ResultsA statistically significant improvement was found in the students wax carving performance following the incorporation of the digital carving activity (p‐value = 0.0001). Students’ performance in the digital carving exercise also statistically improved over the years. Students’ challenges included the technology's unfamiliarity, and a perceived irrelevance of the exercise. Additional guidance, resources, and timely feedback were reported among the students’ support needs during the exercise.ConclusionDigital carving is a promising tool in anatomical education that can improve the students’ spatial understanding and manual dexterity. However, educators need to carefully integrate it into the curriculum to address the students’ challenges and optimize their learning experience.
Proteomic profiling of tumour tissue‐derived extracellular vesicles in colon cancer
Authors: Cvjetkovic Aleksander , Karimi Nasibeh , Crescitelli Rossella , Thorsell Annika , Taflin Helena , Lässer Cecilia , Lötvall Jan 
Published: February 2024

AbstractColon cancer is one of the most commonly occurring tumours among both women and men, and over the past decades the incidence has been on the rise. As such, the need for biomarker identification as well as an understanding of the underlying disease mechanism has never been greater. Extracellular vesicles are integral mediators of cell‐to‐cell communication and offer a unique opportunity to study the machinery that drives disease progression, and they also function as vectors for potential biomarkers. Tumour tissue and healthy mucosal tissue from the colons of ten patients were used to isolate tissue‐resident EVs that were subsequently subjected to global quantitative proteomic analysis through LC‐MS/MS. In total, more than 2000 proteins were identified, with most of the common EV markers being among them. Bioinformatics revealed a clear underrepresentation of proteins involved in energy production and cellular adhesion in tumour EVs, while proteins involved in protein biosynthesis were overrepresented. Additionally, 53 membrane proteins were found to be significantly upregulated in tumour EVs. Among them were several proteins with enzymatic functions that degrade the extracellular matrix, and three of these, Fibroblast activating factor (FAP), Cell surface hyaluronidase (CEMIP2), as well as Ephrin receptor B3 (EPHB3), were validated and found to be consistent with the global quantitative results. These stark differences in the proteomes between healthy and cancerous tissue emphasise the importance of the interstitial vesicle secretome as a major player of disease development.
Both‐strand gene coding in a plastome‐like mitogenome of an enoplid nematode
Authors: Nikolaeva Olga V. , Rusin Leonid Yu , Mikhailov Kirill V. , Aleoshin Vladimir V. , De Ley Paul 
Published: February 2024

AbstractThe phylum Nematoda remains very poorly sampled for mtDNA, with a strong bias toward parasitic, economically important or model species of the Chromadoria lineage. Most chromadorian mitogenomes share a specific order of genes encoded on one mtDNA strand. However, the few sequenced representatives of the Dorylaimia lineage exhibit a variable order of mtDNA genes encoded on both strands. While the ancestral arrangement of nematode mitogenome remains undefined, no evidence has been reported for Enoplia, the phylum's third early divergent major lineage. We describe the first mitogenome of an enoplian nematode, Campydora demonstrans, and contend that the complete 37‐gene repertoire and both‐strand gene encoding are ancestral states preserved in Enoplia and Dorylaimia versus the derived mitogenome arrangement in some Chromadoria. The C. demonstrans mitogenome is 17,018 bp in size and contains a noncoding perfect inverted repeat with 2013 bp‐long arms, subdividing the mitogenome into two coding regions. This mtDNA arrangement is very rare among animals and instead resembles that of chloroplast genomes in land plants. Our report broadens mtDNA taxonomic sampling of the phylum Nematoda and adds support to the applicability of cox1 gene as a phylogenetic marker for establishing nematode relationships within higher taxa.
High‐speed centrifugation rather than Lipoclear reagent can be used for removing the interference of lipemia on serological tests of infectious diseases: AIDS, hepatitis B, hepatitis C, and syphilis by chemiluminescent microparticle immunoassay
Authors: Liu Weiping , Li Lin , Yin Minggang , Cao Chengjian , Song Yaohui , Long Xia 
Published: February 2024

AbstractThe aim of this study was to investigate the interference of lipemia on measurement of HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, anti‐HCV, HIV Ag/Ab, and anti‐TP in serum by chemiluminescent microparticle immunoassay (CMIA) and compare lipemia removing performance between high‐speed centrifugation and Lipoclear reagent. Mixed native serum samples (NSs) and hyperlipemia serum samples (HLS) were prepared for the investigated parameters. The levels of these parameters in NS and HLS were determined by CMIA on an Abbott ARCHITECT i2000SR immunoassay analyzer. HBsAg, anti‐HBs, and anti‐TP were affected with relative bias >12.5% (acceptable limit) when the level of triacylglycerol (TG) was higher than 27.12 mmol/L in HLS. Clinically unacceptable bias were observed for HBeAg and anti‐HBe in HLS with TG higher than 40.52 mmol/L. However, anti‐HCV and HIV Ag/Ab were not interfered in severe lipemia with TG < 52.03 mmol/L. In addition, the Lipoclear reagent did not reduce the interference of lipemia with relative bias from −62.50% to −18.02%. The high‐speed centrifugation under the optimized condition of 12 000g for 10 min successfully removed the interference of lipemia with relative bias from −5.93% to 0% for HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, and anti‐TP. To conclude, high‐speed centrifugation can be used for removing the interference of lipemia to measure HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, and anti‐TP. Accordingly, a standardized sample preanalytical preparation of the patients and other screening participants as well as a specimen examination procedure for removing lipemia interference on the serological tests was recommended.
Genetic characterization and molecular epidemiological analysis of enterovirus C99 in China
Authors: Cong Ruyi , Xiao Jinbo , Ji Tianjiao , Sun Qiang , Lu Huanhuan , Yan Dongmei , Zhu Shuangli , Li Xiaolei , Wang Dongyan , Liu Ying , Li Jichen , Wang Xiaoyi , Yang Tingting , Xu Xizhu , Zhang Yong 
Published: February 2024

AbstractEnterovirus C99 (EV‐C99) is a newly identified EV serotype within the species Enterovirus C. Few studies on EV‐C99 have been conducted globally. More information and research on EV‐C99 are needed to assess its genetic characteristics, phylogenetic relationships, and associations with enteroviral diseases. Here, the phylogenetic characteristics of 11 Chinese EV‐C99 strains have been reported. The full‐length genomic sequences of these 11 strains show 79.4–80.5% nucleotide identity and 91.7–94.3% amino acid (aa) identity with the prototype EV‐C99. A maximum likelihood phylogenetic tree constructed based on the entire VP1 coding region identified 13 genotypes (A–M), revealing a high degree of variation among the EV‐C99 strains. Phylogeographic analysis showed that the Xinjiang Uygur Autonomous Region is an important source of EV‐C99 epidemics in various regions of China. Recombination analysis revealed inter‐serotype recombination events of 16 Chinese EV‐C99 strains in 5′ untranslated regions and 3D regions, resulting in the formation of a single recombination form. Additionally, the Chinese strain of genotype J showed rich aa diversity in the P1 region, indicating that the genotype J of EV‐C99 is still going through variable dynamic changes. This study contributes to the global understanding of the EV‐C99 genome sequence and holds substantial implications for the surveillance of EV‐C99.
Innate immune response to SARS‐CoV‐2 infection contributes to neuronal damage in human iPSC‐derived peripheral neurons
Authors: Passos Vania , Henkel Lisa M. , Wang Jiayi , Zapatero‐Belinchón Francisco J. , Möller Rebecca , Sun Guorong , Waltl Inken , Schneider Talia , Wachs Amelie , Ritter Birgit , Kropp Kai A. , Zhu Shuyong , Deleidi Michela , Kalinke Ulrich , Schulz Thomas F. , Höglinger Günter , Gerold Gisa , Wegner Florian , Viejo‐Borbolla Abel 
Published: February 2024

AbstractSevere acute respiratory coronavirus 2 (SARS‐CoV‐2) causes neurological disease in the peripheral and central nervous system (PNS and CNS, respectively) of some patients. It is not clear whether SARS‐CoV‐2 infection or the subsequent immune response are the key factors that cause neurological disease. Here, we addressed this question by infecting human induced pluripotent stem cell‐derived CNS and PNS neurons with SARS‐CoV‐2. SARS‐CoV‐2 infected a low number of CNS neurons and did not elicit a robust innate immune response. On the contrary, SARS‐CoV‐2 infected a higher number of PNS neurons. This resulted in expression of interferon (IFN) λ1, several IFN‐stimulated genes and proinflammatory cytokines. The PNS neurons also displayed alterations characteristic of neuronal damage, as increased levels of sterile alpha and Toll/interleukin receptor motif‐containing protein 1, amyloid precursor protein and α‐synuclein, and lower levels of cytoskeletal proteins. Interestingly, blockade of the Janus kinase and signal transducer and activator of transcription pathway by Ruxolitinib did not increase SARS‐CoV‐2 infection, but reduced neuronal damage, suggesting that an exacerbated neuronal innate immune response contributes to pathogenesis in the PNS. Our results provide a basis to study coronavirus disease 2019 (COVID‐19) related neuronal pathology and to test future preventive or therapeutic strategies.
Scores predicting atrial fibrillation after mitral valve surgery: Do we need a more specific score?
Authors: Albabtain Monirah A. , Almathami Elham A. , Alghosoon Haneen , Alsubaie Faisal F. , Abdelaal Ibrahim M. , Ismail Huda , Adam Adam I. , Arafat Amr A. 
Published: February 2024

AbstractBackgroundAtrial fibrillation after cardiac surgery (POAF) is associated with increased morbidity and mortality. Several scores were used to predict POAF, with variable results. Thus, this study assessed the performance of several scoring systems to predict POAF after mitral valve surgery. Additionally, we identified the risk factors for POAF in those patients.MethodsThis retrospective cohort included 1381 recruited from 2009 to 2021. The patients underwent mitral valve surgery, and POAF occurred in 233 (16.87%) patients. The performance of CHADS2, CHA2DS2‐VASc, POAF, EuroSCORE II, and HATCH scores was evaluated.ResultsThe median age was higher in patients who developed POAF (60 vs. 54 years; p < .001). CHA2‐DS2‐VASc, POAF, EuroSCORE II, and HATCH scores significantly predicted POAF, with areas under the curve of the receiver operator curve (AUCROC) of 0.56, 0.61, 0.58, and 0.54, respectively. We identified age > 58 years, body mass index > 28 kg/m2, creatinine clearance < 90 mL/min, reoperative surgery, and preoperative inotropic and intra‐aortic balloon pump use as predictors of POAF. We constructed a score from these variables (PSCC‐AF). A score > 2 significantly predicted POAF (p < .001). The AUCROC of this score was 0.67, which was significantly higher than the AUCROC of the POAF score (p = .009).ConclusionPOAF after mitral valve surgery can be predicted based on preoperative patient characteristics. The new PSCC‐AF score significantly predicted POAF after mitral valve surgery and can serve as a bedside diagnostic tool for POAF risk screening. Further studies are needed to validate the PSCC‐AF‐mitral score externally.
Protein‐losing enteropathy secondary to collagenous colitis in a 2‐year‐old
Authors: Khan Hamza Hassan , Lewin David N. , Suppa Carmine 
Published: February 2024

AbstractProtein‐losing enteropathy associated with collagenous colitis (CC) is a rare but described entity in the adult population. However, literature regarding this in the pediatric population is scarce. Here we describe a 2‐year‐old female who presented with fevers, accompanied by nonbloody, watery diarrhea, and decreased oral intake. Work‐up was significant for severe hypoalbuminemia at 1.5 grams per deciliter (g/dL), pancytopenia, and elevated fecal alpha‐1‐antitrypsin at 1.13 milligrams per grams (mg/g). Gastrointestinal mucosal evaluation was normal endoscopically; however, histology was consistent with CC. She responded to 12‐week treatment with budesonide with resolution of symptoms and laboratory values. At this point, she has not had a recurrence 1 year later.
Formation and prediction of heterocyclic amines and N‐nitrosamines in smoked sausages using back propagation artificial neural network
Authors: Zhou Yajun , Zhai Shimin , Yao Guangming , Li Jihong , Li Zongping , Ma Zhiyuan , Ma Qingshu 
Published: February 2024

AbstractBACKGROUNDHeterocyclic amines (HAs) and N‐nitrosamines (NAs) are formed easily during the thermal processing of food, and epidemiological studies have demonstrated that consuming HAs and NAs increases the risk of cancer. However, there are few studies on the application of back propagation artificial neural network (BP‐ANN) models to simultaneously predict the content of HAs and NAs in sausages. This study aimed to investigate the effects of cooking time and temperature, smoking time and temperature, and fat‐to‐lean ratio on the formation of HAs and NAs in smoked sausages, and to predict their total content based on the BP‐ANN model.RESULTSWith an increase in processing time, processing temperature and fat ratio, the content of HAs and NAs in smoked sausages increased significantly, while the content of HA precursors and nitrite residues decreased significantly. The optimal network topology of the BP‐ANN model was 5–11–2, the correlation coefficient values for training, validation, testing and all datasets were 0.99228, 0.99785, 0.99520 and 0.99369, respectively, and the mean squared error value of the best validation performance was 0.11326. The bias factor and the accuracy factor were within acceptable limits, and the predicted values approximated the true values, indicating that the model has good predictive performance.CONCLUSIONThe contents of HAs and NAs in smoked sausages were significantly influenced by the cooking conditions, smoking conditions and fat ratio. The BP‐ANN model has high application value in predicting the contents of HAs and NAs in sausages, which provides a theoretical basis for the suppression of carcinogen formation. © 2024 Society of Chemical Industry.
Soil moisture conditions alter behavior of entomopathogenic nematodes
Authors: Frankenstein Dana , Luu Macawan S , Luna‐Ayala Jennifer , Willett Denis S , Filgueiras Camila S 
Published: February 2024

AbstractBACKGROUNDA variety of environmental factors can disrupt biotic interactions between plants, insects and soil microorganisms with consequences for agricultural management and production. Many of these belowground interactions are mediated by volatile organic compounds (VOCs) which can be used for communication under appropriate environmental conditions. Behavioral responses to these compounds may likewise be dependent on varying soil conditions which are influenced by a changing climate. To determine how changing environmental conditions may affect VOC‐mediated biotic interactions, we used a belowground system where entomopathogenic nematodes (EPNs) – tiny roundworm parasitoids of soil‐borne insects – respond to VOCs by moving through the soil pore matrix. Specifically, we used two genera of EPNs – Heterorhabditis and Steinernema – that are known to respond to four specific terpenes – α‐pinene, linalool, d‐limonene and pregeijerene – released by the roots of plants in the presence of herbivores. We assessed the response of these nematodes to these terpenes under three moisture regimes to determine whether drier conditions or inundated conditions may influence the response behavior of these nematodes.RESULTSOur results illustrate that the recovery rate of EPNs is positively associated with soil moisture concentration. As soil moisture concentration increases from 6% to 18%, substantially more nematodes are recovered from bioassays. In addition, we find that soil moisture influences EPN preference for VOCs, as illustrated in the variable response rates. Certain compounds shifted from acting as a repellent to acting as an attractant and vice versa depending on the soil moisture concentration.CONCLUSIONOn a broad scale, we demonstrate that soil moisture has a significant effect on EPN host‐seeking behavior. EPN efficacy as biological control agents could be affected by climate change projections that predict varying soil moisture concentrations. We recommend that maintaining nematodes as biological control agents is essential for sustainable agriculture development, as they significantly contribute not only to soil health but also to efficient pest management. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.