bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cost-effectiveness and budget impact analysis of lisdexamfetamine versus methylphenidate for patients under 18 with attention-deficit/hyperactivity disorder in Iran
Authors: Tajik Amirmohammad , Nikfar Shekoufeh , Elyasi Sepideh , Rajabi Omid , Varmaghani Mehdi 
Published: October 2023

Abstract
Background
Lisdexamfetamine (LDX) and Methylphenidate (MPH) are stimulant agents that have been shown to provide significant benefits in the management of attention-deficit/hyperactivity disorder (ADHD) in patients.

Aim
This study aimed to assess the cost-effectiveness and the budget impact of LDX compared to MPH as the first-line treatment for ADHD.

Methods
A one-year cost-effectiveness analysis (CEA) was conducted to compare the effects of LDX and MPH in reducing disease symptoms and patient costs and improving quality of life (QoL) from a social perspective. Clinical data were obtained using the EQ-5D questionnaire. In contrast, economic data were sourced from the official website of the Iranian Food and Drug Association (FDA), the national book of tariffs, and specific questionnaires designed to evaluate patients' direct and indirect costs. 197 patients were included in the study, including individuals who sought psychiatric evaluation at a hospital in Mashhad and those who obtained ADHD medications from governmental pharmacies. The cost-effectiveness of the study medicine was assessed using the decision tree method, and the results were presented as the Incremental Cost-Effectiveness Ratio (ICER). Deterministic Sensitivity Analysis (DSA) and Probabilistic Sensitivity Analysis (PSA) were performed to assess the robustness of the findings. Additionally, a Budget Impact Analysis (BIA) was conducted over five years, considering three different scenarios, to evaluate the financial implications of incorporating LDX into the national pharmaceutical system.

Results
The ICER for LDX therapy compared to MPH was estimated at USD 264.28 (with an incremental cost of USD 54.9, incremental effectiveness of 0.208, and Quality-Adjusted Life Years (QALYs) gained of 0.765). The PSA indicated a 0.994% probability of LDX being cost-effective, considering a threshold of USD 2450 per QALY. Furthermore, the DSA revealed that the acquisition cost of LDX influenced the model's sensitivity. The BIA demonstrated that incorporating LDX into Iran's healthcare system would result in a financial burden of approximately $368,566 in the first year, representing an additional cost of $11,154 compared to the non-availability of this medicine and the use of previous medications. It is projected that by 2027, the financial burden of treating ADHD with LDX will reach approximately USD 443,879 over five years, amounting to an increase of $71,154 compared to the absence of this medicine.

Conclusion
From a social perspective, the inclusion of LDX in the treatment regimen for ADHD is associated with higher costs and an increased financial burden. However, based on our analysis, LDX appears to be a cost-effective choice for managing ADHD in Iran when compared to MPH.
Facile synthesis and eco-friendly analytical methods for concurrent estimation of selected pharmaceutical drugs in their solutions: application to quality by design, lean six sigma, and stability studies
Authors: Al-Kadhi Nada S. , Mohamed Mahmoud A. , Ahmed Hoda A. , Nassar Hossam F. 
Published: October 2023

AbstractEconomical, highly robust, selective, precise, and eco-friendly RP-UPLC and spectrophotometric methods were developed and validated for the concurrent estimation of selected pharmaceutical drugs represented in ceftazidime (CFZ) and pyridine (PYD) in their solutions using Agilent Zorbax SB-C18 RRHD (50 × 2.1 mm, 1.8 μm) column at flow rate 0.3 mL/min with wavelength 254 nm. Box-Behnken design (BBD) established Response surface methodology (RSM) to achieve the optimum chromatographic condition with minimal trials conducted. Three independent variables specifically acetonitrile ratio 60–70%, pH 3–7, and temperature 25–35 °C were implemented to evaluate the influences of these variables on the responses as resolution and retention time. Desirability and overlay plots were carried out to adjust the optimal condition that achieved the shortest retention time of less than 2 min and desired resolution of more than 1.5 using a mobile phase consisting of acetonitrile: purified water (70:30, v/v) at pH 5.0 adjusted by 0.1% orthophosphoric acid with the column oven temperature 30 °C and column void volume 0.46 mL. Mean centering of ratio spectra (MCR) and ratio subtraction (RS) methods were effectively applied to resolve drugs' spectral superposition at 220 nm, 255.4 nm, 260.3 nm, and 254.6 nm for CFZ and PYD, respectively. Linearity range was accomplished for UPLC, MCR, and RS methods over the concentration range of 2–100, 1–50,3–30 and 5–30 µg/mL for CFZ and PYD, respectively with correlation coefficient > 0.999 and good recovery results within 98–102%. Six Sigma methodology was achieved using the process capability index (Cpk) to compare the suggested and USP methods showing that both are highly capable with Cpk > 1.33. The proposed method was successfully validated depending on ICH guidelines and ANOVA results and applied for the accelerated stability study.
Graphical Abstract
Genomic, transcriptomic and epigenomic sequencing data of the B-cell leukemia cell line REH
Authors: Lysenkova Wiklander Mariya , Övernäs Elin , Lagensjö Johanna , Raine Amanda , Petri Anna , Wiman Ann-Christin , Ramsell Jon , Marincevic-Zuniga Yanara , Gezelius Henrik , Martin Tom , Bunikis Ignas , Ekberg Sara , Erlandsson Rikard , Larsson Pontus , Mosbech Mai-Britt , Häggqvist Susana , Hellstedt Kerje Susanne , Feuk Lars , Ameur Adam , Liljedahl Ulrika , Nordlund Jessica 
Published: October 2023

Abstract
Objectives
The aim of this data paper is to describe a collection of 33 genomic, transcriptomic and epigenomic sequencing datasets of the B-cell acute lymphoblastic leukemia (ALL) cell line REH. REH is one of the most frequently used cell lines for functional studies of pediatric ALL, and these data provide a multi-faceted characterization of its molecular features. The datasets described herein, generated with short- and long-read sequencing technologies, can both provide insights into the complex aberrant karyotype of REH, and be used as reference datasets for sequencing data quality assessment or for methods development.

Data description
This paper describes 33 datasets corresponding to 867 gigabases of raw sequencing data generated from the REH cell line. These datasets include five different approaches for whole genome sequencing (WGS) on four sequencing platforms, two RNA sequencing (RNA-seq) techniques on two different sequencing platforms, DNA methylation sequencing, and single-cell ATAC-sequencing.
Validity of a multiphase CT-based radiomics model in predicting the Leibovich risk groups for localized clear cell renal cell carcinoma: an exploratory study
Authors: Liu Huayun , Wei Zongjie , Xv Yingjie , Tan Hao , Liao Fangtong , Lv Fajin , Jiang Qing , Chen Tao , Xiao Mingzhao 
Published: October 2023

Abstract
Objective
To develop and validate a multiphase CT-based radiomics model for preoperative risk stratification of patients with localized clear cell renal cell carcinoma (ccRCC).

Methods
A total of 425 patients with localized ccRCC were enrolled and divided into training, validation, and external testing cohorts. Radiomics features were extracted from three-phase CT images (unenhanced, arterial, and venous), and radiomics signatures were constructed by the least absolute shrinkage and selection operator (LASSO) regression algorithm. The radiomics score (Rad-score) for each patient was calculated. The radiomics model was established and visualized as a nomogram by incorporating significant clinical factors and Rad-score. The predictive performance of the radiomics model was evaluated by the receiver operating characteristic curve, calibration curve, and decision curve analysis (DCA).

Results
The AUC of the triphasic radiomics signature reached 0.862 (95% CI: 0.809–0.914), 0.853 (95% CI: 0.785–0.921), and 0.837 (95% CI: 0.714–0.959) in three cohorts, respectively, which were higher than arterial, venous, and unenhanced radiomics signatures. Multivariate logistic regression analysis showed that Rad-score (OR: 4.066, 95% CI: 3.495–8.790) and renal vein invasion (OR: 12.914, 95% CI: 1.118–149.112) were independent predictors and used to develop the radiomics model. The radiomics model showed good calibration and discrimination and yielded an AUC of 0.872 (95% CI: 0.821–0.923), 0.865 (95% CI: 0.800–0.930), and 0.848 (95% CI: 0.728–0.967) in three cohorts, respectively. DCA showed the clinical usefulness of the radiomics model in predicting the Leibovich risk groups.

Conclusions
The radiomics model can be used as a non-invasive and useful tool to predict the Leibovich risk groups for localized ccRCC patients.

Critical relevance statement
The triphasic CT-based radiomics model achieved favorable performance in preoperatively predicting the Leibovich risk groups in patients with localized ccRCC. Therefore, it can be used as a non-invasive and effective tool for preoperative risk stratification of patients with localized ccRCC.

Key points
• The triphasic CT-based radiomics signature achieves better performance than the single-phase radiomics signature.
• Radiomics holds prospects in preoperatively predicting the Leibovich risk groups for ccRCC.
• This study provides a non-invasive method to stratify patients with localized ccRCC.

Graphical Abstract
Advances in stem cell therapy for peritoneal fibrosis: from mechanisms to therapeutics
Authors: Huang Weiyan , Xia Demeng , Bi Wendi , Lai Xueli , Yu Bing , Chen Wei 
Published: October 2023

AbstractPeritoneal fibrosis (PF) is a pathophysiological condition caused by a variety of pathogenic factors. The most important features of PF are mesothelial–mesenchymal transition and accumulation of activated (myo-)fibroblasts, which hinder effective treatment; thus, it is critical to identify other practical approaches. Recently, stem cell (SC) therapy has been indicated to be a potential strategy for this disease. Increasing evidence suggests that many kinds of SCs alleviate PF mainly by differentiating into mesothelial cells; secreting cytokines and extracellular vesicles; or modulating immune cells, particularly macrophages. However, there are relatively few articles summarizing research in this direction. In this review, we summarize the risk factors for PF and discuss the therapeutic roles of SCs from different sources. In addition, we outline effective approaches and potential mechanisms of SC therapy for PF. We hope that our review of articles in this area will provide further inspiration for research on the use of SCs in PF treatment.
Regulatory T cells contribute to the immunosuppressive phenotype of neutrophils in a mouse model of chronic lymphocytic leukemia
Authors: Goral Agnieszka , Sledz Marta , Manda-Handzlik Aneta , Cieloch Adrianna , Wojciechowska Alicja , Lachota Mieszko , Mroczek Agnieszka , Demkow Urszula , Zagozdzon Radoslaw , Matusik Katarzyna , Wachowska Malgorzata , Muchowicz Angelika 
Published: October 2023

Abstract
Background
Impaired neutrophil activity is an important issue in chronic lymphocytic leukemia (CLL), as it contributes to a dysfunctional immune response leading to life-threatening infections in patients. Some features typical of CLL neutrophils, e.g., the B-cell-supportive secretion profile, have already been described. However, most of these studies were performed on cells isolated from peripheral blood. It is still unclear which molecular factors and cell types are involved in shaping neutrophil function and phenotype in the CLL microenvironment. Since regulatory T cells (Treg) play an important role in CLL progression and influence the activity of neutrophils, we investigated the crosstalk between Treg and neutrophils in the spleen using a murine model of CLL.

Methods
In this work, we used an Eµ-TCL1 mouse model of human CLL. For our in vivo and ex vivo experiments, we inoculated wild-type mice with TCL1 leukemic cells isolated from Eµ-TCL1 transgenic mice and then monitored disease progression by detecting leukemic cells in peripheral blood. We analyzed both the phenotype and activity of neutrophils isolated from the spleens of TCL1 leukemia-bearing mice. To investigate the interrelation between Treg and neutrophils in the leukemia microenvironment, we performed experiments using TCL1-injected DEREG mice with Treg depletion or RAG2KO mice with adoptively transferred TCL1 cells alone or together with Treg.

Results
The obtained results underline the plasticity of the neutrophil phenotype, observed under the influence of leukemic cells alone and depending on the presence of Treg. In particular, Treg affect the expression of CD62L and IL-4 receptor in neutrophils, both of which are crucial for the function of these cells. Additionally, we show that Treg depletion and IL-10 neutralization induce changes in the leukemia microenvironment, partially restoring the “healthy” phenotype of neutrophils.

Conclusions
Altogether, the results indicate that the crosstalk between Treg and neutrophils in CLL may play an important role in CLL progression by interfering with the immune response.
Advanced numerical techniques for time integration of relativistic equations of motion for charged particles
Authors: Umeda Takayuki , Ozaki Riku 
Published: October 2023

AbstractAdvanced numerical techniques for solving the relativistic equations of motion for charged particles are provided. A new fourth-order integrator is developed by combining the Taylor series expansion of the numerical angle of relativistic gyration and the fourth-order Runge–Kutta method for integrating the Lorentz factor. The new integrator gives the exact relativistic E-cross-B drift velocity, but has a numerical accuracy much higher than the classic fourth-order Runge–Kutta integrator.
Graphical Abstract
Resumption of production operations during the silent disposal period of African swine fever outbreaks: a case study
Authors: Hu Zhiqiang , Liu Qingyuan , Tian Xiaogang , Li Lulu , Wu Weisheng , Gao Wenchao , Li Xiaowen 
Published: October 2023

Abstract
Background
Batch production, a widely implemented production model in large-scale pig farms, was characterized by its long-term duration, cost-effectiveness, and efficiency. Nevertheless, the recent occurrence of African swine fever (ASF) outbreaks in China has necessitated the implementation of discreet mating operations within this model, leading to disruptions in production cycles and substantial indirect losses.

Case presentation
This study implemented a novel operational procedure, which involved the division of risk areas for zone management and allowed mating operations, in 12 farms experiencing ASF outbreaks. Another 12 farms were used as a control group, employing the old procedure. Subsequently, the prognoses of both the old and new procedures were calculated and analyzed. The findings indicate that the new method resulted in an enhanced retention rate and reduced non-productive days (NPD), without impacting the positive detection rate and disposal time. Consequently, this approach significantly mitigated economic losses (p < 0.05).

Conclusion
The efficacy of the novel procedure in mitigating the indirect economic losses stemming from ASF outbreaks, through the reduction of NPD while maintaining retention rates and disposition days, has been substantiated. This methodology has demonstrated feasibility in extensive pig farming operations and exhibits promise for broader application.
Inventory analysis of fire effects wrought by wind-driven megafires in relation to weather and pre-fire forest structure in the western Cascades
Authors: Busby Sebastian U. , Klock Angela M. , Fried Jeremy S. 
Published: October 2023

Abstract
Background
Six synchronous, wind-driven, high severity megafires burned over 300,000 hectares of mesic temperate forest in the western Cascades of NW Oregon and SW Washington states in early September 2020. While remote sensing data has been utilized to estimate fire severity across the fires, assessments of fire impacts informed by field observations are missing. We compiled field measurement data, pre- and post-fire, from a statistically representative sample of existing forest inventory analysis (FIA) plots, to estimate stand-level fire effects indices that describe (1) tree survival and its implications for carbon emissions, (2) effects on tree crowns, and (3) effects on soils. Field observations were analyzed in relation to fire weather when plots burned and to evaluate accuracy of remotely sensed burn severity classifications.

Results
Wind speed strongly interacted with tree size and stand age to influence tree survival. Under high fuel aridity but light winds, young stands composed of small trees, found primarily on private lands, exhibited a much lower survival rate than older stands composed of medium to large trees, found primarily on federal lands. Under moderate to high winds, poor tree survival was characteristic of all forest structures and ownerships. Fire impacts on tree crowns were strongly related to wind speed, while fire impacts on soils were not. These fires transferred nearly 70 MMT CO2e from wood in live and growing trees to a combination of immediate smoke and carbon emissions, plus delayed emissions from dead wood, that will release most of the embodied carbon over the next few decades. These emissions will exceed all 2020 anthropogenic emissions in Oregon (64 MMT CO2e). Substantial discrepancies were observed between two remotely sensed burn severity products, BAER-SBS and MTBS-TC, and field observed soil organic matter cover and tree mortality, respectively.

Conclusions
Post-fire FIA plot remeasurements are valuable for understanding fire’s impact on forest ecosystems and as an empirical basis for model validation and hypothesis testing. This continuous forest inventory system will compound the value of these post-fire remeasurements, enabling analysis of post-fire forest ecosystem trajectories in relation to both immediate fire impacts and pre-fire conditions.
Deep structural controls on the distribution of carbonate reservoirs and overburden heterogeneity in Central Luconia province, offshore Borneo revealed by 3D anisotropic inversion of regional controlled-source electromagnetic and magnetotelluric profile data
Authors: Kho Joanna H. W. , Meju Max A. , Miller Roger V. , Saleh Ahmad Shahir 
Published: October 2023

Understanding the deep structural controls on reservoir distribution and overburden heterogeneity is important for near-field exploration and green-field development of the Miocene to Holocene carbonate buildups in Central Luconia province in offshore northwest Borneo, Malaysia. Data from 59 stations along an approximately 180 km long controlled-source electromagnetic (CSEM) and magnetotelluric (MT) survey line with three segments recorded using different CSEM transmitter-towing directions were available. We applied three-dimensional (3D) anisotropic resistivity inversion with cross-gradient constraint and verified the resulting models using resistivity logs from nearby wells and the acoustic basement interpreted from seismic data. Our anisotropic resistivity models reveal a fragmented carbonate-rich zone atop a segmented basement comprising electrically resistive horsts coinciding with the northerly Mega-platform, Central and Southern Field Highs, separated by steep conductive zones coinciding with the West, East, and Southeast Troughs previously interpreted from seismic data. The structural highs correlate with the spatial distribution of the known carbonate buildups implying a genetic link. The carbonate bodies are overlain and underlain by persistent layers (C2 and C3) of low resistivity and high anisotropy, which we interpret as indicating compressional deformation or detachment zones. Overburden layer C2 (whose base coincides with the top of a key sedimentary package, Cycle V in seismic data) is thinnest over the East Trough and is discontinuous at the eastern margin of the West Trough, which are locations where drilled wells did not find hydrocarbons implying that the seal rocks are inefficient or breached at those localities. We used these observations to refine the existing seismic-based interpretation of carbonate play-types along our transect indicating how 3D joint CSEM-MT imaging can potentially contribute to derisking or optimizing future exploration and/or development work in this province.
Laboratory Performance Testing of Aqueous Nasal Inhalation Products for Droplet/Particle Size Distribution: an Assessment from the International Pharmaceutical Aerosol Consortium on Regulation and Science (IPAC-RS)
Authors: Doub William H. , Suman Julie M. , Copley Mark , Goodey Adrian P. , Hosseini Sana , Mitchell Jolyon P. 
Published: October 2023

AbstractAlthough nasal inhalation products are becoming more and more important for the delivery of medicines, characterization of these products for quality control and assessment of bioequivalence is complicated. Most of the problems encountered are associated with the assessment of aerodynamic droplet/particle size distribution (APSD). The droplets produced by the various nasal devices are large, and for suspension products, individual droplets may contain multiple drug particles or none at all. Assessment of suspension products is further complicated by the presence of solid excipient particles. These complications make it imperative that the limitations of the instruments used for characterization as well as the underlying assumptions that govern the interpretation of data produced by these instruments are understood. In this paper, we describe various methodologies used to assess APSD for nasal inhalation products and discuss proper use, limitations, and new methodologies on the horizon.
Glycosphingolipids are linked to elevated neurotransmission and neurodegeneration in a Drosophila model of Niemann Pick type C
Authors: Eberwein Anna E. , Kulkarni Swarat S. , Rushton Emma , Broadie Kendal 
Published: October 2023

ABSTRACT
The lipid storage disease Niemann Pick type C (NPC) causes neurodegeneration owing primarily to loss of NPC1. Here, we employed a Drosophila model to test links between glycosphingolipids, neurotransmission and neurodegeneration. We found that Npc1a nulls had elevated neurotransmission at the glutamatergic neuromuscular junction (NMJ), which was phenocopied in brainiac (brn) mutants, impairing mannosyl glucosylceramide (MacCer) glycosylation. Npc1a; brn double mutants had the same elevated synaptic transmission, suggesting that Npc1a and brn function within the same pathway. Glucosylceramide (GlcCer) synthase inhibition with miglustat prevented elevated neurotransmission in Npc1a and brn mutants, further suggesting epistasis. Synaptic MacCer did not accumulate in the NPC model, but GlcCer levels were increased, suggesting that GlcCer is responsible for the elevated synaptic transmission. Null Npc1a mutants had heightened neurodegeneration, but no significant motor neuron or glial cell death, indicating that dying cells are interneurons and that elevated neurotransmission precedes neurodegeneration. Glycosphingolipid synthesis mutants also had greatly heightened neurodegeneration, with similar neurodegeneration in Npc1a; brn double mutants, again suggesting that Npc1a and brn function in the same pathway. These findings indicate causal links between glycosphingolipid-dependent neurotransmission and neurodegeneration in this NPC disease model.