bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of an instrument to evaluate the success of Electronic Health Records adoption in a hospital setting
Authors: RHAYHA RADOUANE , Ismaili Abderrahman Alaoui 
Published: October 2023

Abstract

Background: Evaluating healthcare information systems, such as the Electronic Health Records (EHR), is both challenging and essential, especially in resource-limited countries. This study aims to psychometrically develop and validate an instrument (questionnaire) to assess the factors influencing the successful adoption of the EHR system by healthcare professionals in Moroccan university hospitals.
Methods: The questionnaire validation process occurred in two main stages. Initially, data collected from a pilot sample of 164 participants underwent analysis using exploratory factor analysis (EFA) to evaluate the validity and reliability of the retained factor structure. Subsequently, the validity of the overall measurement model was confirmed using confirmatory factor analysis (CFA) in a sample of 368 healthcare professionals.
Results: The structure of the modified HOT-fit model, comprising seven constructs (system quality, information quality, IT service quality, user satisfaction, organization, environment, and clinical performance), was confirmed through confirmatory factor analysis. Absolute, incremental, and parsimonious fit indices all indicated an appropriate level of acceptability, affirming the robustness of the measurement model. Additionally, the instrument demonstrated adequate reliability and convergent validity, with composite reliability values ranging from 0.75 to 0.89 and average variance extracted (AVE) values ranging from 0.51 to 0.63. Furthermore, the square roots of AVE values exceeded the correlations between different pairs of constructs, and the heterotrait-monotrait ratio of correlations (HTMT) was below 0.85, confirming suitable discriminant validity.
Conclusions: The resulting instrument, due to its rigorous development and validation process, can serve as a reliable and valid tool for assessing the success of information technologies in similar contexts.
Amino-acid PET for monitoring post Stupp protocol temozolomide therapy in high-grade glioma patients
Authors: Zinsz Adeline , Ahrari Shamimeh , Becker Jason , Mortada Ali , Roch Veronique , Doriat Louis , Santi Matthieu , Blonski Marie , Taillandier Luc , Zaragori Timothée , Verger Antoine 
Published: October 2023

Abstract
Few studies have to date investigated the value of amino-acid PET for monitoring temozolomide (TMZ) therapy in high-grade glioma (HGG). This study aimed to evaluate the performance of amino-acid PET for monitoring HGG patients at the time of TMZ treatment discontinuation, after the Stupp protocol.
Methods: The analysis included consecutive HGG patients with dynamic 18F-FDOPA PET imaging within 3 months of the end of TMZ therapy, post Stupp protocol. Static and dynamic PET parameters, responses to RANO criteria for MRI and clinical and histo-molecular factors were correlated to progression-free (PFS) and overall survival (OS). Metabolic tumor volumes (MTVs) were defined semi-automatically with a 1.6 threshold from the healthy brain.
Results: Forty-three patients (60.6 [53.6;68.6] years of age, 16 (37%) women), 38 (88.4%) with IDH-wildtype glioblastoma, having received 6 to 12 TMZ cycles were included. MTV was associated with an 81% of accuracy for the definition of PFS at 6 months (p<0.001). In the multivariate analysis, MTV was an independent predictor of PFS in association with the MRI response assessment (HR=1.09 [1.01-1.17], p=0.021). The presence of an MTV was associated with a lower PFS (5.3 vs. 16.0 months) and OS (20.7 vs. not reached).
Conclusion: Amino-acid PET is an efficient tool for monitoring TMZ therapy in HGG patients having undergone a Stupp protocol. In these patients, the absence of MTV after 6 to 12 cycles of temozolomide constitutes a favorable prognostic factor.
Protein restriction during pregnancy alters Cdkn1c silencing, dopamine circuitry and behaviour in offspring without wholescale disruption of neuronal gene expression
Authors: Prodani Chiara , Irvine Elaine E. , Sardini Alessandro , Gleneadie Hannah J. , Dimond Andrew , de Pette Mathew Van , John Rosalind , Howes Oliver , Withers Dominic J. , Ungless Mark A. , Merkenschlager Matthias , Fisher Amanda G. 
Published: October 2023

Abstract
We tracked the consequences of in utero protein restriction in mice throughout their development and life course using a luciferase-based allelic reporter of imprinted Cdkn1c. Exposure to gestational low-protein diet (LPD) results in the inappropriate expression of paternally inherited Cdkn1c in the brains of embryonic and juvenile mice. These animals were characterised by a developmental delay in motor skills, and by behavioural alterations indicative of reduced anxiety. Exposure to LPD in utero resulted in significantly more tyrosine hydroxylase positive (dopaminergic) neurons in the midbrain of adult offspring as compared to age-matched, control-diet equivalents. Positron emission tomography (PET) imaging revealed an increase in striatal dopamine synthesis capacity in LPD-exposed offspring, where elevated levels of dopamine correlated with an enhanced sensitivity to cocaine. These data highlight a profound sensitivity of the developing epigenome to gestational protein restriction. Our data also suggest that loss of Cdkn1c imprinting and p57KIP2 upregulation alter the cellular composition of the developing midbrain, compromises dopamine circuitry, and thereby provokes behavioural abnormalities in early postnatal life. Molecular analyses revealed that despite this phenotype, exposure to LPD solely during pregnancy did not cause a gross perturbation in neuronal- or dopamine-associated gene expression that was sustained into adulthood.
Predictive Value of ApoB/ApoA-I for recurrence within 1 year after first incident stroke
Authors: Lin Jie , Si Zhihua , Wang Aihua 
Published: October 2023

Abstract
ApoB/ApoA-I ratio is a reliable indicator of cholesterol balance, particularly in the prediction of ischemic events risk. The aim of this study was to investigate the prognostic value of ApoB/ApoA-I for stroke recurrence within 1 year after the first incident. We retrospectively included patients who were first diagnosed with acute (< 7 days after onset) ischemic stroke. Blood samples were collected on admission, and serum ApoB and ApoA-I concentrations were measured. We analyzed the relationship between ApoB/ApoA-I ratio and ischemic stroke recurrence within 1 year. A total of 722 patients with acute ischemic stroke were included, of whom 102 experienced stroke recurrence within 1 year, with a recurrence rate of 14.1%. Serum ApoB/ApoA-I concentrations on admission were higher in patients with stroke recurrence at 1 year compared with those with a good prognosis (P < 0.001). The Kaplan–Meier survival curve revealed a significant difference in cumulative stroke recurrence rates across ApoB/ApoA-I tertiles (log-rank P value < 0.001). A positive correlation between the ApoB/ApoA-I ratio and the risk of stroke recurrence within one year was demonstrated using Cox regression analysis, which remained significant after adjusting for traditional risk factors (hazard ratio [HR] 4.007, 95% confidence interval [CI] 1.661–9.666). This relationship was particularly strong in patients with LAA stroke (HR 4.955, 95% CI 1.591–15.434). Subgroup analysis further revealed that a high ApoB/ApoA-I ratio was strongly associated with stroke recurrence regardless of whether patients had high or low LDL-C levels. ApoB/ApoA-I ratio, measured during the acute phase of the first stroke, was positively correlated with the risk of stroke recurrence within one year.
Patient Perceptions of Altering Chemotherapy Treatment Due to Peripheral Neuropathy
Authors: Hertz Daniel L , Tofthagen Cindy , Rossi Emanuela , Bernasconi Davide Paolo , Lim Jiyoon , Carlson Martha , Sheffield Katharine E. , Nekhlyudov Larissa , Grech Lisa , Von Ah Diane , Mayo Samantha , Ruddy Kathryn J , Chan Alexandre , Alberti Paola , Lustberg Maryam B , Tanay Mary 
Published: October 2023

Abstract
Purpose Clinical practice guidelines recommend altering neurotoxic chemotherapy treatment in patients experiencing intolerable chemotherapy-induced peripheral neuropathy (CIPN). The primary objective of this survey was to understand patient’s perspectives on altering neurotoxic chemotherapy treatment, including their perceptions of the benefits of preventing irreversible CIPN and the risks of reducing treatment efficacy.Methods A cross-sectional online survey was distributed via social networks to patients who were currently receiving or had previously received neurotoxic chemotherapy for cancer. Survey results were analyzed using descriptive statistics and qualitative analysis.Results Following data cleaning, 447 participants were included in the analysis. The median age was 57 years, 93% were white, and most were from the United Kingdom (53%) or United States of America (38%). Most participants who were currently or recently treated expected some CIPN symptom resolution (86%), but 45% of those who had completed treatment more than a year ago reported experiencing no symptom resolution. Participants reported that they would discontinue chemotherapy treatment for less severe CIPN if they knew their symptoms would be permanent than if symptoms would disappear after treatment. Most patients stated that the decision to alter chemotherapy or not was usually made collaboratively between the patient and their treating clinician (61%). The most common reason participants were reluctant to talk with their clinician about CIPN was fear that treatment would be altered. Participants noted a need for improved understanding of CIPN symptoms and their permanence, better patient education relating to CIPN prior to and after treatment, and greater clinician understanding and empathy around CIPN.Conclusions This survey highlights the importance of shared decision-making, including a consideration of both the long-term benefits and risks of altering neurotoxic chemotherapy treatment due to CIPN. Additional work is needed to develop decision aids and other communication tools that can be used to improve shared decision making and help patients with cancer achieve their treatment goals.
CD83 expression in Adult Acute Lymphoblastic Leukemia: correlation with clinical outcome
Authors: Mohamed Haydi Sayed , Hassan Noha Bassiouny , Annaka Layla Mahmoud , Abduallah Nour El Hoda Hussein 
Published: October 2023

Abstract
Background: CD83 is not expressed by peripheral blood cells except mature dendritic cells and some activated lymphocytes. It is expressed with high concentration in chronic lymphocytic leukaemia, adult acute lymphoblastic leukaemia and lymphomas.
Aim: to detect the percentage of CD83 on lymphoblast in peripheral blood of adult ALL patients by flowcytometry and to investigate its prognostic role with correlation to clinical outcome as well as response to treatment and survival.
Patients and Methods: We analysed CD83 expression by flowcytometry on peripheral blood lymphoblast in 40 adults newly diagnosed ALL patients and on normal lymphocytes in 20 healthy controls of matched age and sex.
Results: Our patients exhibited a statistically significant increase in CD83 expression on their peripheral blood lymphoblast compared to lymphocytes of the control group [median 19.5 % (IQR 5.4-48.15%) vs 7 % (IQR 6.1-8.5%), respectively, p=0.012]. Patients were divided into two groups: a group with high CD83 (>9.8%) and the other with normal CD83 expression (≤9.8%) compared to the control group using ROC curve. Complete remission was achieved in 18(69.2%) patients with higher CD83 expression vs 7(50%) patients with normally expressed CD83. The median (IQR) of the overall survival and disease-free survival was longer than those normally expressed CD83 versus those with higher expression [180(IQR 60-240) &105(IQR 30-160) vs 80(IQR 60-240) &51(IQR 22-150) days, respectively.
Conclusion: CD83 is a novel prognostic test in ALL patients and acts as a predictor for response to treatment.
Understanding the Factors Associated with COVID-19 Vaccine Hesitancy in Venezuela
Authors: Chacón-Labrador Fabián R. , Passantino María G. , Moncada-Ortega Augusto , Ávila Atahualpa A. , Moreno Andrea A. , Kuffaty-Akkou Nicolle A. , Pedroza Luisana M. , Camejo-Ávila Natasha A. , Mendoza-Millán Daniela L. , Rodriguez-Saavedra Carlis M. , Marcano-Rojas María V. , Hernández-Medina Fernando , Grillet María E. , Carrión-Nessi Fhabián S. , Forero-Peña David A. 
Published: October 2023

Abstract

Background
Despite nearly a quarter of Venezuelans remaining unvaccinated against coronavirus disease 2019 (COVID-19), the factors contributing to vaccine hesitancy in the country have not been thoroughly investigated.
Methods
A cross-sectional study was conducted from October 15th to 30th, 2022, using a knowledge, attitudes, and practices (KAP) survey to identify factors associated with COVID-19 vaccine hesitancy.
Results
The study analyzed data from 1,930 participants across 24 states in Venezuela. The majority (93.4%) were vaccinated. The mean age was 40 years, predominantly female (67.3%), and held a university degree (70.6%). The mean KAP score was significantly higher among vaccinated individuals compared to unvaccinated ones (7.79 vs. 3.94 points for knowledge, 40 vs. 24 points for attitude, and 16 vs. 10 points for practice, all p < 0.001). Increases in the scores for KAP were associated with increased odds of being vaccinated (84.6%, 25.6%, and 33% respectively for each one-point increase, all p < 0.001). Certain demographic factors such as marital status, occupation, religious beliefs, monthly income, and location influence COVID-19 vaccine knowledge. Higher income and certain occupations decrease the odds of low knowledge, while residing in specific states increases it. Attitudes towards the COVID-19 vaccine are influenced by age, health status, vaccination status, and location. Higher income and absence of certain health conditions decrease the odds of negative attitudes. Lastly, age, occupation, monthly income, and location affect vaccine practices. Advanced age and higher income decrease the odds of inappropriate practices, while residing in La Guaira state increases them.
Conclusion
Factors such as age, education level, occupation, monthly income, and location were found to be associated with knowledge and attitudes towards COVID-19 vaccine among the surveyed Venezuelans.
The Sensitivity and Resistance Risk Assessment of Coniella vitis to Mefentrifluconazole
Authors: LI Bao-yan , SHI Jie , ZHOU Hua-fei , ZHANG Wei , CHEN Ping , ZHANG Zi-ran , LIU Bao-you 
Published: October 2023

Abstract
Coniella vitis, one important fungal pathogen, causes grape white rot which can significantly reduce yield and quality of grape. Fungicide application is the main control measure of grape white rot. To evaluate the resistance and risk of C. vitis in Shandong province to mefentrifluconazole, in this experiment, the sensitive baseline of C. vitis to mefentrifluconazole was established, and resistance risk of the pathogen was evaluated by analyzing the genetic stability, biological characteristics, and interaction resistance with other fungicides of the mefentrifluconazole resistant strain. The field evaluation suggested that mefentrifluconazole is an excellent fungicide for control of C. vitis on grape. The EC50 of 76 strains of C. vitis to mefentrifluconazole was ranged from 0.0210 to 59.0305 μg/mL. The frequency distribution map of EC50 of 64 strains of C. vitis in the first main peak against mefentrifluconazole compound normal distribution, the average EC50 is 0.7409±0.4346 μg/mL, which can be used as the sensitive baseline for C. vitis in Shandong province to mefentrifluconazole. There had resistant isolates to mefentrifluconazole emerged in the field, sharing for 3.95% of the total tested strains. No difference was observed between the resistant and sensitive strains in terms of temperature sensitivity. The average rate of mycelial growth, spore production and pathogenicity of resistant strains did not decrease compared to sensitive strains, and some resistant strains were even higher than sensitive strains. The correlation coefficent of the logarithms of EC50 values to C. vitis between mefentrifluconazole and other fungicides, including pyraclostrobin, thiram, mancozeb, carbendazol, imazalil, difenoconazole, propiconazole and tebuconazole, were -0.0812, 0.1024, -0.2865, -0.2178, 0.3198, 0.5598, 0.5511 and 0.3977. Indicating that there was no significant cross resistance between mefentrifluconazole and the above 8 fungicides. This study lays a foundation for the resistance risk assessment of C. vitis to mefentrifluoconaole.
Optimization of pacing parameters to entrain slow wave activity in the pig jejunum
Authors: Nagahawatte Nipuni D. , Avci Recep , Paskaranandavadivel Niranchan , Cheng Leo K. 
Published: October 2023

Abstract
Pacing has been proposed as a therapy to restore function in motility disorders associated with electrical dysrhythmias. The spatial response of bioelectrical activity in the small intestine to pacing is poorly understood due to a lack of high-resolution investigations. This study systematically varied pacing parameters to determine the optimal settings for the spatial entrainment of slow-wave activity in the jejunum. An electrode array was developed to allow simultaneous pacing and highresolution mapping of the small intestine. Pacing parameters including pulsewidth (50, 100 ms), pulse-amplitude (2, 4, 8 mA) and pacing electrode orientation (antegrade, retrograde, circumferential) were systematically varied and applied to the jejunum (n = 15 pigs). Pulse-amplitudes of 4 mA (p = 0.012) and 8 mA (p = 0.002) were more effective than 2 mA in achieving spatial entrainment while pulse-widths of 50 ms and 100 ms had comparable effects (p = 0.125). A pulsewidth of 100 ms and a pulseamplitude of 4 mA was determined to be most effective for slow-wave entrainment when paced in the antegrade or circumferential direction with a success rate of greater than 75%. These settings can be applied in chronic studies to evaluate the long-term efficacy of pacing, a critical aspect in determining its therapeutic potential.
Investigating the role of cognitive load on prospective memory performance across event and mixed cues retrievals
Authors: Ojha P , Kashyap N 
Published: October 2023

Abstract
Prospective memory (PM) is remembering to perform a delayed action in the future. A number of reminders can initiate prospective memories (future events and actions). At any given time, people plan to execute several tasks in the future, the success of which depends on the successful retrieval of reminders for actions. Such scenarios justify the use of multiple reminders as this would increase the chance of success in future task completion. The present study explores one such possibility where two reminders in conjugation (mixed) signal future task execution. To know the effectiveness of multiple reminders on future task performance we tested scenarios with simple and complex task paradigms. We hypothesize multiple reminders guarantee higher success rates for future task completion however, this benefit would decrease with increase in complexity of future task. The result of our study suggests that multiple reminder improve chances of success for both simple and complex task future tasks with simple tasks showing higher improvement complex tasks.
A Human Tooth-On-Chip Model Shows Long-Range Osteoinduction from the Dentine Surface
Authors: Macalester William , Moreno Rafael , Shanon Mark , West Nicola , Hill Darryl , Boussahel Asme , Perriman Adam 
Published: October 2023

Abstract

Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral defects. However, the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering, exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems, which will impact on cell fate and subsequent treatment efficacy. Herein, we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells (hMSCs) encapsulated in a microporous hydrogel bioink. We demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs, and that both dentine and b-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface, which is distal to the gel-substrate interface. Our findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provides a framework for the rapid development of 3D dentine-bone interface models.
A prognostic gene signature based on focal adhesion related genes for gliomas and identification of the role of RAP1B in glioma progression
Authors: Wang Ning , Zhou Haoyu , Wang Tianze , Li Xiaodong , Wang Yichang , Wu Wei , Xiang Jianyang , Chen Xiao , Zhang Beichen , wu pancheng , Zhou Mingjing , Cao Yiyang , Yu Hai , Bao Gang 
Published: October 2023

Abstract
Background
The most common malignant primary brain tumor in adults is the gliomas, characterized by extremely variable overall survival (OS) for patients. Although it has been found that focal adhesion genes are associated with clinical prognosis in glioma patients, this marker is rarely used clinically.
Methods
We systematically characterized mRNA expression of focal adhesion related genes in gliomas and explored their expression signature based on 938 samples from TCGA dataset and CGGA dataset. Glioma samples were clustered using mRNA expression of focal adhesion genes using an unsupervised clustering method. Subsequently, based on prognosis-associated genes, the focal adhesion related gene signature (FARGS) was constructed by least absolute shrinkage and selection operator (LASSO) Cox regression. Additionally, multiple bioinformatics methods were used to examine the value of FARGS in predicting patient outcomes, clinical features, oncogenic pathways, tumor immune microenvironment and drug response. Furthermore, in vitro and in vivo experiments were conducted to validate the role of RAP1B in U87 glioma cells.
Results
According to LASSO Cox regression analysis, a 9-FARG signature was found to be strongly linked with OS in glioma patients, characterized by a high-risk and a low-risk score pattern. The FARGS was found to be tightly linked with malignant molecular biomarkers, including IDH wild-type, unmethylated MGMTp, and non-codeletion of 1p19q. Furthermore, the high-risk group exhibited an enrichment of multiple oncogenic biological pathways. Interestingly, the results presented that the FARGS has a strong association with therapeutic response and tumor immunosuppressive microenvironment in gliomas, including immune infiltrations of M2-type macrophages, MDSCs and Tregs, and elevated immunosuppressors’ mRNA expression. Lastly, the oncogenic role of RAP1B in U87 glioma cells was also functionally confirmed.
Conclusions
In conclusion, we reported a novel FARGS with promising survival prediction for glioma patients, as well as confirmation of RAP1B's oncogenic role.