bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case of de novo neuroendocrine prostate cancer presented with elevated level of serum CEA carrying BRCA2 mutation: case report and literature review
Authors: Han Weizhe , Rexiati Nihati , Yu Fang , Wang Yongzhi , Tian Yueli , Wu Jianyuan , Wang Gang , Liu Tao , Yang Zhonghua 
Published: January 2025

BackgroundDe novo neuroendocrine prostate cancer (NEPC) is a rare subtype of prostate cancer (PCa) and few markers are available for screening and monitoring. Potential circulating or fluid markers might facilitate early diagnosis thus improving prognosis of NEPC, especially for de novo NEPC.Case presentationA man of 71-year was presented with elevated level of serum carcinoembryonic antigen (CEA) (1296.5 ng/ml) and normal PSA (0.47ng/ml). Gastrointestinal endoscopy showed no signs of gastric or colorectal cancer. Fluorodeoxyglucose positron emission tomography-computed tomography (FDG PET-CT) and prostate-specific membrane antigen PET-CT (PSMA PET-CT) indicated prostate cancer with metastases including pelvic lymph nodes, bone as well as lung metastases. Biopsy of prostate revealed mixed carcinoma including small cell neuroendocrine carcinoma (SCNEC) and adenocarcinoma (Gleason score of 4 + 5). Immunohistochemistry (IHC) staining and next generation sequencing demonstrated a strong expression of chromogranin A (CgA), synaptophysin (SYN) and CEA, and a germline mutation in BRCA2, respectively. After a prostatic massage, an increased level of CEA (137 ng/ml vs 5 ng/ml) was detected in urine. Olaparib, a Poly ADP-ribose polymerase inhibitor (PARPi), combined with androgen deprivation therapy (ADT) were administrated. FDG PET-CT indicated tumor regression in both quantity and size three months later, and CEA levels of serum and urine decreased to 23 ng/ml and 2.4 ng/ml 4 months later, respectively.ConclusionThis is the first report of a de novo NEPC presented with an elevated level of CEA, in which CEA was also detected in urine specimen post a prostatic massage. After a combination treatment of ADT for 3 months, levels of CEA in both serum and urine decreased sharply when tumor regressed radiologically. CEA might be a marker of screening and monitoring of NEPC.
Cerebellar metastasis from colorectal cancer: a case report
Authors: Angulo-Rojo Carla E. , Castillo-Gaxiola Luis J. , Gaxiola-Gastélum Karen , Guadrón-Llanos Alma M. , Magaña-Gómez Javier A. , Baldenebro-Félix Diana L. 
Published: January 2025

IntroductionColorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide, with adenocarcinoma as the most common subtype. While metastasis typically occurs in the liver, lungs, and peritoneal cavity, metastasis to the brain, particularly the cerebellum, is exceedingly rare.Case presentationThis report discusses the case of a 50-year-old woman diagnosed with mucinous adenocarcinoma of the descending colon. Over six years, the patient experienced multiple common metastatic sites, including the liver and lungs, before developing a rare cerebellar metastasis. Despite extensive treatment, including surgery and chemotherapy, the disease progressed, ultimately leading to the patient’s demise. This case represents the first documented cerebellar metastasis from CRC in Mexico.ConclusionThis case highlights the altered metastatic patterns in CRC due to advanced therapies that extend survival. Clinicians should remain vigilant for metastasis to uncommon sites, such as the cerebellum, especially in patients with prolonged survival. Further research is needed to understand the mechanisms underlying such metastatic behavior and optimize treatment strategies.
MDTR: a knowledge-guided interpretable representation for quantifying liver toxicity at transcriptomic level
Authors: Sung Inyoung , Lee Sangseon , Bang Dongmin , Yi Jungseob , Lee Sunho , Kim Sun 
Published: January 2025

IntroductionDrug-induced liver injury (DILI) has been investigated at the patient level. Analysis of gene perturbation at the cellular level can help better characterize biological mechanisms of hepatotoxicity. Despite accumulating drug-induced transcriptome data such as LINCS, analyzing such transcriptome data upon drug treatment is a challenging task because the perturbation of expression is dose and time dependent. In addition, the mechanisms of drug toxicity are known only as literature information, not in a computable form.MethodsTo address these challenges, we propose a Multi-Dimensional Transcriptomic Ruler (MDTR) that quantifies the degree of DILI at the transcriptome level. To translate transcriptome data to toxicity-related mechanisms, MDTR incorporates KEGG pathways as representatives of mechanisms, mapping transcriptome data to biological pathways and subsequently aggregating them for each of the five hepatotoxicity mechanisms. Given that a single mechanism involves multiple pathways, MDTR measures pathway-level perturbation by constructing a radial basis kernel-based toxicity space and measuring the Mahalanobis distance in the transcriptomic kernel space. Representing each mechanism as a dimension, MDTR is visualized in a radar chart, enabling an effective visual presentation of hepatotoxicity at transcriptomic level.Results and DiscussionIn experiments with the LINCS dataset, we show that MDTR outperforms existing methods for measuring the distance of transcriptome data when describing for dose-dependent drug perturbations. In addition, MDTR shows interpretability at the level of DILI mechanisms in terms of the distance, i.e., in a metric space. Furthermore, we provided a user-friendly and freely accessible website (http://biohealth.snu.ac.kr/software/MDTR), enabling users to easily measure DILI in drug-induced transcriptome data.
Yunvjian decoction attenuates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB/NLRP3 pathway and pyroptosis
Authors: Zhang Fanxuan , Wang Fang , Zhao Lisha , Wang Leqian , Li Wenjing , Huang Feihua , Wang Nani 
Published: January 2025

IntroductionYunvjian (YNJ) decoction, a classic traditional Chinese medicine prescription for inflammatory diseases, has demonstrated good therapeutic effects in the clinical treatment of pneumonia. The aim of this study was to clarify the effective ingredients and mechanism of action of YNJ on lipopolysaccharide (LPS)-induced acute lung injury (ALI).MethodsThe effects of YNJ were evaluated in a mouse model of LPS-induced ALI and in LPS-treated MLE-12 murine lung epithelial cells and RAW264.7 macrophages in vitro. The mechanism of action of YNJ on these model systems was studied using RNA sequencing, immunohistochemical analysis, immunoblotting, immunofluorescence, ELISA, and polymerase chain reaction assays. Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied to identify the absorbed components of YNJ.ResultsYNJ attenuated pulmonary damage in LPS-treated mice, as evidenced by reduced protein content in bronchoalveolar lavage fluid, decreased lung wet/dry weight ratio, and improved respiratory function. Analysis of pneumonia-related lung injury samples from patients in the Gene Expression Omnibus dataset GSE40012 indicated that NOD-like receptor protein 3 (NLRP3)-mediated pyroptosis was a primary mechanism in ALI. YNJ reduced the phosphorylation of nuclear factor-kappa B (NF-κB) and decreased the expression levels of lung NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), cleaved caspase-1, and interleukin-1β levels (IL-1β) in vivo. Administration of YNJ-containing mouse serum increased cell viability and decreased malondialdehyde and reactive oxidative species contents in LPS-stimulated MLE-12 cells. YNJ-containing serum also decreased the secretion of tumor necrosis factor-α, IL-6, and IL-1β in LPS-stimulated RAW264.7 macrophages, and promoted macrophage polarization toward an M2 phenotype. A total of 23 absorbed components were identified in YNJ-containing serum. Among those, network analysis and in vitro experiments indicated that diosgenin, timosaponin BII, and mangiferin are anti-inflammatory active substances.ConclusionYNJ attenuates LPS-induced ALI in mice by inhibiting pyroptosis of lung epithelial cells and macrophages via suppression of the NF-κB/NLRP3 pathway. Our findings provide novel insights into the therapeutic effects of YNJ on ALI.
Adverse events of hepatic anti-fibrotic agents in phase 3 and above clinical trials: a descriptive analysis of the WHO-VigiAccess database
Authors: Liu Yuwei , Zhao Xu , Wang Xinrui , Zhou Qiang 
Published: January 2025

IntroductionLiver fibrosis is a pathological condition in response to chronic liver injuries. Currently, there is no Food and Drug Administration (FDA) approved pharmacotherapy for liver fibrosis. Advances in understanding hepatic fibrogenesis have led to the development of anti-fibrotic agents, and some of them have shown promise in phase 3 and above clinical trials. However, adverse drug reactions (ADRs) associated with emerging anti-fibrotic agents may hinder their efficacy and clinical applicability. This study assessed ADRs associated with anti-fibrotic agents as reported in the World Health Organization (WHO) VigiAccess database and compared the adverse reaction characteristics of these agents for optimizing therapeutic strategies.MethodsA detailed search was conducted on ClinicalTrial.gov to identify phase 3 or 4 clinical trials involving hepatic anti-fibrotic agents. The ADR reports were retrieved from the WHO-VigiAccess database, with data categorized by demographic characteristics, geographic distribution, and System Organ Classes (SOCs). The most frequently reported ADRs were identified through descriptive analysis. Disproportionality analysis, measured by reporting odd ratio (ROR) and proportional reporting ratio (PRR), was performed to evaluate ADRs related to gastrointestinal disorders.ResultsFive hepatic anti-fibrotic agents (empagliflozin, liraglutide, candesartan, obeticholic acid, and resmetirom) were identified. A total of 130,567 ADR reports were analyzed, with empagliflozin, liraglutide, and candesartan showing significantly higher ADRs. The most frequently reported SOCs included gastrointestinal disorders (29.44%), general disorders (24.12%), and nervous system disorders (14.42%). Liraglutide demonstrated a higher risk of gastrointestinal ADRs (ROR: 4.629, 95% CI: 4.517–4.744; PRR: 3.566, 95% CI: 3.492–3.642) compared to the other agents. Severe ADRs were reported in empagliflozin, such as ketoacidosis and infections, while liraglutide was associated with pancreatitis and candesartan with acute kidney injury. Serious ADR rates varied, with candesartan reporting the highest proportion (7.28%).ConclusionWhile hepatic anti-fibrotic agents showed promise in addressing liver fibrosis, their ADR profiles underscore the importance of pharmacovigilance and personalized treatment approaches. Future efforts should focus on improving the pharmacovigilance system, expanding population diversity in trials, and conducting ongoing research and extensive post-marketing surveillance.
Domestication of medicinal plants (Lonicera japonica Thunb.) in China: comparison of morphological, resistance and biochemical traits between wild and cultivated populations
Authors: Hou Congzhe , Huang Luyao , Li Zhuangzhuang , Sun Nan , Yang Sheng , Li Jia , Liu Zhenhua 
Published: January 2025

BackgroundMost studies on domesticated plants have focused on those utilized for sustenance purposes. This study provides valuable insights into the domestication processes of honeysuckle (Lonicera japonica Thunb.), a semi-evergreen twining vine in the Caprifoliaceae family that is important in traditional Chinese medicine for its flower buds and potential as a dietary supplement. The definition of domestication syndrome for honeysuckle remains unclear due to its perennial and asexual reproduction mode, resulting in a lack of information on domestication-related traits. Therefore, this study aims to compare and analyze differences in morphology, resistance, and biochemical composition between wild and cultivated varieties.MethodsA total of 36 wild and 81 cultivated specimens were examined to compare plant morphology, flowering time, bud length, active ingredients, and resistance between wild and cultivated populations.ResultsThe cultivated honeysuckle exhibited several noteworthy characteristics, including more erect plant morphology, higher flower-to-leaf ratio, more obvious aggregation of flowers into clusters at the top of branches, higher frequency of flowering, and longer bud length compared to the wild type. Additionally, the cultivated specimen demonstrated significantly elevated levels of chlorogenic acid as a biochemical constituent. However, in terms of resistance against powdery mildew, wild individuals displayed evident advantages over their cultivated counterparts.ConclusionThe observed phenotypic variation within the honeysuckle field provides empirical support for the hypothesis that farmer management practices influence domestication syndrome, as indicated by the deliberate enhancement of specific desirable traits during species domestication.
How does career-related parental support benefit career adaptability of medical imaging technology students in Asia-Pacific LMICs? The roles of psychological capital and career values
Authors: Zhang Xiaohui , Shen Yangjing , Xu Junxiang , Cui Guanyu 
Published: January 2025

BackgroundChina, as a low- and middle-income country (LMIC) in the Asia-Pacific region, is advancing its rural medical and health system. Students of Medical Imaging Technology (MIT) in China, who will probably be employed in rural, are facing the pressure from both study and employment. Previous results proposed that career-related parent support and psychological capital could positively influence the level of career adaptability, while career-related parent support affects career values. This study aimed to explore the impact of career-related parental support on career adaptability and the mechanism between them, as well as the role of psychological capital and career values.MethodsA total of 520 (80.8% female) participants were recruited from MIT students in China, using Questionnaires about career-related parental support, psychological capital, career values, career adaptability. Path analysis was conducted using Mplus 8.3.ResultsThere was a significant correlation between career adaptability and career-related parental support, psychological capital and career values. Career-related parental support could positively predict students’ career adaptation. Psychological capital and career values played a parallel mediating role between career-related parental support and career adaptation.ConclusionThe results demonstrate that it is necessary to enhance career-related parental support to improve MIT students’ career adaptability by the enrichment of psychological capital and the refinement of career values, on the basis of the popularization of medical career programs among the public and medical literacy courses in colleges.
Perception in context of Chinese and Japanese: the role of language proficiency
Authors: Lu Sa , Ren Rongxia , Guo Ting , Tang Xiaoyu 
Published: January 2025

IntroductionThe effect of language context on bilinguals has been studied in phonetic production. However, it is still unclear how the language context affects phonetic perception as the level of second language (L2) proficiency increases.MethodsChinese–Japanese auditory cognates were selected to avoid the interference of semantics and font or spelling processing. Low- to high-proficiency Chinese–Japanese bilinguals, as well as Chinese and Japanese monolinguals, were asked to judge whether the initial morpheme of the Chinese or Japanese words was pronounced with the vowels /a/ or /i/ in single- and mixed-language contexts.ResultsThe results found that low-proficiency bilinguals judged vowels faster in the single-language context than in the mixed-language context, whereas high-proficiency bilinguals showed no significant difference between the single- and mixed-language contexts. DiscussionThese results indicate that as language proficiency increases, bilinguals appear to adaptively enhance phonetic perception when faced with different control demands in single-language and mixed-language contexts.
Risk factors for electroconvulsive therapy-induced fever: a retrospective case-control study
Authors: Deng Can-Jin , Yang Jian-Wen , Liu Zi-Zhe , Ning Ting , Nie Sha , Huang Xiong , Yang Xin-Hu , Huang Xing-Bing , Zheng Wei 
Published: January 2025

BackgroundElectroconvulsive therapy (ECT)-induced fever can significantly affect patient experience, treatment adherence, and the course of treatment. However, little is known about the prevalence of ECT-induced fever and its associated risk factors in patients with major mental disorders (MMD).MethodsThis retrospective, case-control study included 113 cases and 226 age-matched controls (1:2). The case group comprised patients who exhibited an axillary temperature of ≥37.5°C (99.5°F) at least once within 24 hours post-ECT. Patients diagnosed with MMD underwent ECT between January 1, 2021, and December 31, 2021, at a large psychiatric hospital in China. Demographic and clinical data were extracted from the electronic chart management system (ECMS) for both groups.ResultsThe prevalence of ECT-induced fever in patients with MMD was 6.8% [113/1,674, 95% confidence interval (CI): 5.6% to 8.0%], with a session-based prevalence of 1.1% (130/11,570, 95% CI: 0.9% to 1.3%). Multivariate logistic regression analysis identified paliperidone [odds ratios (OR)=2.5, 95% CI: 1.2 to 4.9] as a risk factor, while quetiapine (OR=0.4, 95% CI: 0.3 to 0.8) was found to be protective. No significant association between etomidate and ECT-induced fever was observed in univariate analysis (p&gt;0.05).ConclusionsThis study found a relatively low prevalence of ECT-induced fever. Paliperidone was identified as a risk factor, while quetiapine had a protective effect. Etomidate was not significantly associated with ECT-induced fever in patients with MMD.
Bibliometric and visual analysis of suicide in aged people over the past 20 years
Authors: Kan Shao-Kui , Zhang Ying-Li , Bai Xue-Xia , Peng Bo 
Published: January 2025

BackgroundThe rapid aging of the global population presents pressing public health challenges, notably an increase in suicide rates among older adults. Despite this critical issue, there is a scarcity of comprehensive assessments regarding the existing literature on suicide within this demographic. This study seeks to employ bibliometric analysis and knowledge mapping to elucidate prevailing research trends and the overall status of this field.MethodsWe performed a comprehensive computer-based search of the Web of Science Core Collection to identify relevant articles and reviews concerning suicide in the elderly. A bibliometric analysis was conducted, examining various aspects including countries or regions, institutions, authors, journals, keywords, and references. This analysis utilized tools such as CiteSpace, VOSViewer, Pajek, and Excel 365 to facilitate a thorough assessment.ResultsThe analysis covered 1,116 publications from 2005 to 2024. The annual number of publications showed a fluctuating upward trend, with notable decreases in 2007, 2012, 2015, 2017, and 2022, and increases in 2009, 2013, 2016, and 2019, peaking at 121 in 2021, with citations reaching 4,741 in 2022, but declining since then. The United States stands out as the most productive and influential country in the field, boasting the highest number of publications and citations. The country is home to institutions leading in both publication and citation metrics. Prominent authors contributing significantly to this domain include Conwell Yeates, Van Orden Kimberly A., and Waern Margda. Key journals disseminating crucial research are the Journal of Affective Disorders, American Journal of Geriatric Psychiatry, and Lancet. Frequent keywords in this field encompass depression, suicidal ideation, suicide, older-adults, risk, risk factors, prevalence, older adults, ideation, behavior, health, mental health, life, age, people, prevention, symptoms, scale, population, and elderly. The contemporary research emphasis is primarily on identifying, treating, and preventing the suicide risk associated with depression in older adults.ConclusionsThis study highlights the growing research focus on suicide in older adults, particularly related to depression and the identification, treatment, and prevention of suicide risk. The findings underscore the need for targeted prevention strategies and further investigation in this critical public health area.
The availability and distribution of health services and resources across different regions in Afghanistan
Authors: Hegazy Nelly , El Deeb Sherif , Salem Marwa Rashad , Shaguy Jerome A. , Mohammed Ramesh Nassery , Khawari Abdullah , Tanoli Jamshed , Abouzeid Alaa 
Published: January 2025

BackgroundEnsuring equitable access to healthcare services is fundamental to a robust healthcare system, especially during humanitarian crises. This study analyzes the availability and distribution of health resources across Afghanistan, aiming to provide a data-driven understanding of healthcare resource access and identify potential disparities, a critical aspect of effective humanitarian response planning.MethodsPrincipal investigators collated related data and literature from databases and data warehouses in a systematic approach using search strings and collection tools to query databases and available data warehouses to assess the availability and distribution of health services and resources across different regions in Afghanistan, with the principal database queried being the Afghanistan Health Resources Availability database (HeRAMS), an electronic and web-based system conceived by the World Health Organization. An Excel version was sourced.ResultsThe sub-health center represents 31.1% of the health facilities, followed by the basic health center (22.5%) and the mobile health team (17.1%). More than 85% of these facilities are fully operational, with the highest percentage observed in the Southern and Northeastern regions at 96.8%, followed closely by the central highland and Southeastern regions. Outpatient services for primary care are notably prevalent in the Northeastern, Southeastern, Southern, Eastern, and Northern region, conversely, the Capital and Central Highland regions demonstrate the lowest provision of primary care services. Antenatal care services are accessible at a level exceeding 70% in nearly all regions, with the highest accessibility in the Northeastern region at 91.3%, the prevalence of non-communicable illness clinics was observed to be below 50%, with the highest availability in the Southern region at 49.4%, followed by the Southeastern region. In terms of sanitation facilities, availability surpasses 70% across Afghanistan, with the highest observed in the Northeastern region at 89.2%.ConclusionThe study highlights significant disparities in healthcare access across various regions, with notable challenges in the availability of critical services. Furthermore, the study underscores the significant impact of financial constraints and equipment shortages on the functionality of healthcare facilities, particularly in the Northeastern and Western regions. This analysis emphasises the need for targeted resource allocation and infrastructure improvements to address inequities in access to essential healthcare services, particularly for underserved populations, thereby facilitating the achievement of equitable health outcomes in Afghanistan.