bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periodic oscillations of Doppler frequency excited by the traveling ionospheric disturbances associated with the Tonga eruption in 2022
Authors: Nakata Hiroyuki , Hosokawa Keisuke , Saito Susumu , Otsuka Yuichi , Tomizawa Ichoro 
Published: October 2023

AbstractThe explosive eruption of the Hunga Tonga-Hunga Ha’apai volcano on 15 January 2022 generated atmospheric waves traveling around the Earth, which caused ionospheric disturbances on various spatio-temporal scales. A HF Doppler sounding system in Japan detected characteristic ionospheric disturbances showing periodic oscillations in the Doppler frequency with a period of ~ 4 min. In this study, such periodic oscillations were examined by comparing Doppler frequency data with Total Electron Content data obtained by Global Navigation Satellite System. The observed periodic oscillations in the Doppler frequency were characterized by a sawtooth or S-letter shaped variation, implying the passage of the traveling ionospheric disturbances through the reflection points of the HF Doppler sounding system. It was also found that the periodic oscillations occurred prior to the arrival of the tropospheric Lamb wave excited by the Tonga eruption. From the total electron content data, the traveling ionospheric disturbances causing the periodic oscillations were excited by the tropospheric Lamb waves at the conjugate point in the southern hemisphere, namely, the electric field perturbations due to the Lamb waves in the southern hemisphere mapped onto the sensing area of the HF Doppler sounding system in the northern hemisphere along the magnetic field lines. The periodic oscillations were observed only in the path between Chofu transmitter and Sarobetsu receiver, whose the radio propagation path is almost aligned in the north–south direction. This suggests that the traveling ionospheric disturbance has a structure elongating in the meridional direction. The variation in the Doppler frequency was reproduced by using a simple model of the propagation of the traveling ionospheric disturbances and the resultant motion of the reflection point. As a result, the vertical motion of the reflection point associated with the periodic oscillations was estimated to be about 1 km. It is known that 4-min period variations are sometimes observed in association with earthquakes, which is due to resonances of acoustic mode waves propagating between the ground and the lower ionosphere. Therefore, a similar resonance structure in the southern hemisphere is a plausible source of the traveling ionospheric disturbances detected in the northern hemisphere.
Graphical Abstract
Detection of hidden earthquakes after the 2011 Tohoku earthquake by automatic hypocenter determination combined with machine learning
Authors: Tamaribuchi Koji , Kudo Shota , Shimojo Kengo , Hirose Fuyuki 
Published: October 2023

AbstractAfter the 2011 Mw 9.0 Tohoku earthquake, seismicity became extremely active throughout Japan. Despite enormous efforts to detect the large number of earthquakes, microearthquakes (M < 2 inland, M < 3 offshore) were not always cataloged and many have remained undetected, making it difficult to understand the detailed seismicity after the 2011 Tohoku earthquake. We developed an automatic hypocenter determination method combined with machine learning to detect microearthquakes. Machine learning was used for phase classification with convolutional neural networks and ensemble learning to remove false detections. We detected > 920,000 earthquakes from March 2011 to February 2012, triple the number of the conventional earthquake catalog (~ 320,000). This represents a great improvement in earthquake detection, especially in and around the Tohoku region. Detailed analysis of our merged catalog more clearly revealed features such as (1) swarm migrations, (2) small foreshock activity, and (3) increased microseismicity preceding repeating earthquakes. This microseismic catalog provides a magnifying glass for understanding detailed seismicity.
Graphical Abstract
Interference screws manufactured from magnesium display similar primary stability for soft tissue anterior cruciate ligament graft fixation compared to a biocomposite material – a biomechanical study
Authors: Deichsel Adrian , Glasbrenner Johannes , Raschke Michael J. , Klimek Matthias , Peez Christian , Briese Thorben , Herbst Elmar , Kittl Christoph 
Published: October 2023

AbstractPurposeBiodegradable interference screws (IFS) can be manufactured from different biomaterials. Magnesium was previously shown to possess osteoinductive properties, making it a promising material to promote graft‐bone healing in anterior cruciate ligament reconstruction (ACLR). The purpose of this study was to compare IFS made from magnesium to a contemporary biocomposite IFS.MethodsIn a porcine model of ACL reconstruction, deep porcine flexor tendons were trimmed to a diameter of 8 mm, sutured in Krackow technique, and fixed with either 8 × 30 mm biocomposite IFS (Bc‐IFS) or 8 × 30 mm magnesium IFS (Mg‐IFS) in an 8 mm diameter bone tunnel in porcine tibiae. Cyclic loading for 1000 cycles from 0 to 250 N was applied, followed by load to failure testing. Elongation, load to failure and stiffness of the tested constructs was determined.ResultsAfter 1000 cycles at 250 N, elongation was 4.8 mm ± 1.5 in the Bc‐IFS group, and 4.9 mm ± 1.5 in the Mg‐IFS group. Load to failure was 649.5 N ± 174.3 in the Bc‐IFS group, and 683.8 N ± 116.5 in the Mg‐IFS group. Stiffness was 125.3 N/mm ± 21.9 in the Bc‐IFS group, and 122.5 N/mm ± 20.3 in the Mg‐IFS group. No significant differences regarding elongation, load to failure and stiffness between Bc‐IFS and Mg‐IFS were observed.ConclusionMagnesium IFS show comparable biomechanical primary stability in comparison to biocomposite IFS and may therefore be an alternative to contemporary biodegradable IFS.
Update on prevalence and antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot ulcers in Africa: a systematic review and meta-analysis
Authors: Makeri Danladi , Odoki Martin , Eilu Emmanuel , Agwu Ezera 
Published: October 2023

Abstract
Background
Diabetes mellitus is increasing in Africa, and diabetes-related amputations exacerbated by diabetic foot infection are also prevalent with Staphylococcus aureus and Pseudomonas aeruginosa two priority pathogens playing key roles. Understanding the local epidemiology and antimicrobial resistance profiles of these dominant pathogens is crucial for appropriate antibiotic therapy.

Main body of abstract
This systematic review and meta-analysis aim to contribute valuable insights that can guide the management of diabetic foot ulcer-related infections in Africa by comprehensively analyzing the available literature on the prevalence and antimicrobial resistance profiles of Staphylococcus aureus and Pseudomonas aeruginosa in infected diabetic foot ulcers across Africa. We conducted a continent-based literature search utilizing PubMed and Scopus databases on June 11th, 2023, to identify studies conducted in Africa among persons with diabetic foot ulcers that reported isolating bacteria from the foot ulcers. The main concepts related to this research, “diabetic foot ulcers”, “diabetic foot infections”, “bacteria” and “Africa” were expanded with their synonyms and combined using Boolean operators (AND, OR) to formulate the final search query. The selection and inclusion of studies followed the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA). Our review revealed that approximately 4124 bacteria have been isolated from diabetic foot ulcers across 13 African countries. Staphylococcus aureus is the dominant species with a random effect pooled prevalence of 19.9% (95%CI: [16.19–23.84%]; I2 = 88.56% [82.26–92.62%]) followed by Pseudomonas aeruginosa with 11.8% (95%CI: [8.67–15.23%]; I2 = 89.95% [84.67–93.41%]). Methicillin-resistant Staphylococcus aureus (MRSA) pooled 12.9% (95%CI: [3.99–25.89%]; I2 = 95.47% [93.68–96.75%]). Multidrug-resistant S.aureus and P. aeruginosa pooled prevalence is 26.4% (95%CI: [17.84–36.06%]; I2 = 71.16% [49.34–83.58%]) and 41.8% (95%CI: [27.38–56.91%]; I2 = 78.48% [60.80–88.18%]), respectively.

Short conclusion
Staphylococcus aureus dominates diabetic foot ulcer (DFU) isolates in Africa contrary to the prevailing assertion about Pseudomonas aeruginosa. However, multidrug resistance among both species is high emphasizing the need for antimicrobial stewardship and utilization of other wound management protocols such as topical silver sulfadiazine (SSD) for the duo.
Ecosystem type and species’ traits help explain bird responses to spatial patterns of fire
Authors: Rainsford Frederick W. , Giljohann Katherine M. , Bennett Andrew F. , Clarke Michael F. , MacHunter Josephine , Senior Katharine , Sitters Holly , Watson Simon , Kelly Luke T. 
Published: October 2023

AbstractBackgroundUnderstanding how temporal and spatial attributes of fire regimes, environmental conditions, and species’ traits interact to shape ecological communities will help improve biodiversity conservation in fire-affected areas. We compared the influence of time since the last fire at a site, and the area and diversity of post-fire successional vegetation surrounding a site (i.e., the “spatial context” of fire), on bird species and functional groups in two ecosystems in south-eastern Australia. These ecosystems, semi-arid “mallee” woodlands and temperate “foothill” forests, differ in stand-regeneration patterns, climate, and topography. For 22 bird species in mallee woodlands, 33 species in foothill forests and four functional groups of birds in both ecosystems, we fitted non-linear models that differed in fire regime predictor variables.ResultsIn foothill forests, models that included both time since fireanda spatial context variable explained more variation in bird abundances than models that included only time since fireora spatial variable. In mallee woodlands, the addition of spatial attributes of fire helped explain the occurrence of several species, but this finding was muted when measured across all species. There were key differences between ecosystems in functional group responses to fire regimes. Canopy/upper-midstorey foragers were positively associated with the amount oflate-successional vegetation in mallee woodlands, but not in foothill forests. Lower-midstorey foragers showed a decline response to the amount oflate-successional vegetation in mallee woodlands and a contrasting incline response in foothill forests. However, lower-midstorey foragers showed a similar response to the amount of surroundingearly-successional vegetation in both ecosystems—decreasing in abundance when > 50% of the surrounding vegetation was early-successional.ConclusionsThe influence of fire regimes on birds varies among species within sites, across landscapes and between ecosystems. Species’ foraging traits influence bird associations with fire regimes, and help to make sense of a myriad of relationships, but are usefully understood in the context of ecosystem types and the regeneration patterns of their dominant flora. The spatial context of fire regimes is also important—the amount of successional vegetation surrounding a site influences bird abundance. Fire management strategies that incorporate the spatial contexts of fire regimes, as well as the temporal and ecological contexts of fire regimes, will have the greatest benefits for biodiversity.
Rice yellow stunt virus p3 protein enters the nucleus of leafhopper cell and localizes to viroplasm through interaction with N protein via importin α3-mediated pathway
Authors: Huang Zhejun , Ji Zhenxi , Wang Juan , Li Zhanpeng , Jiang Zhoumian , Ni Wei , Chen Hongyan , Wei Taiyun , Zhang Xiao-Feng 
Published: October 2023

AbstractRice yellow stunt virus (RYSV) P3 protein functions as a movement protein during viral infection of a plant host; its function in insect hosts remains unclear. In this study, we investigated the subcellular localization of P3 using leafhopper (Nephotettix cincticeps) cell cultures. Our results showed that P3 translocated from the cytoplasm to the nucleus in RYSV-infected leafhopper cells, where it interacted with the viral N protein as a constituent of viroplasms. Interfering with the P3 gene expression significantly suppressed viral infection in N. cincticeps. Finally, we demonstrate that the nuclear translocation of P3 in leafhopper cells depended on its interaction with RYSV N protein, which enters the nucleus via an interaction with importin α3. These findings unveil a previously unknown role for P3 in RYSV infection of the insect vector and provide valuable insights into the infection dynamics of plant rhabdoviruses.
Compound Bacillus alleviates diarrhea by regulating gut microbes, metabolites, and inflammatory responses in pet cats
Authors: Wang Fei , Mei Xiaoying , Wang Qi , Zhao Pengwei , Zhou Yuanhao , Tang Li , Wang Baikui , Xu Shujie , Li Xiang , Jin Qian , Xiao Yingping , Li Weifen 
Published: October 2023

Abstract
Background
Pet cats frequently have diarrhea in their daily life. Bacillus has a protective role that has crucial beneficial functions on intestinal homeostasis. The aim of this research was to investigate the effects of the compound Bacillus on the prevention of diarrhea, microbiota and metabolism in pet cats. A total of 20 pet cats (1–2 years old, 3.91 ± 0.92 kg) were randomly divided into two groups and fed with a basal diet (Control group), or a basal diet supplemented with 3 × 109 CFU/kg compound Bacillus (Probiotics group). The experiment lasted 33 days.

Results
Results showed that the compound Bacillus significantly reduced the rate of soft stools and diarrhea in pet cats compared with the control group (P < 0.05, n = 10). Meanwhile, compared with the control group, the probiotics group significantly decreased the content of IL-1β and IL-6 and significantly increased IL-10 (P < 0.05, n = 6) in the serum. In addition, feeding probiotics significantly increased the abundance of p_Patescibacter and g_Plectosphaerella, decreased the abundance of p_Firmicutes, p_Gemmatimonadetes, g_Ruminococcaceae_UCG-005, g_Ascochytahe and g_Saccharomyces in the feces of the pet cats (P < 0.05, n = 6). And it also can significantly increase the content of total SCFAs, acetic acid and butyric acid in the feces (P < 0.05, n = 6). The fecal and serum metabolomics analyses revealed that most fecal and serum compounds were involved in metabolism, particularly in chemical structure transformation maps and amino acid metabolism. Also, eugenitol and methyl sulfate were the most significantly increased serum metabolites, and log2FC were 38.73 and 37.12, respectively. Pearson’s correlation analysis showed that changes in serum metabolism and fecal microbiota were closely related to immune factors. There was also a strong correlation between serum metabolites and microbiota composition.

Conclusions
The results of this research highlight the potential of the compound Bacillus as a dietary supplement to alleviate diarrhea in pet cats.
Isolated pulmonary artery involvement in Takayasu arteritis: case report and review of the literature
Authors: Li Jie , Xu Jun , Bao Ping , Li Hongmei 
Published: October 2023

Abstract
Background
Takayasu arteritis (TAK) is a chronic inflammatory arteritis. It most often affects non-specific large vessel progressively, such as the aorta and its branches. The diagnosis in TAK is typically delayed. Isolated pulmonary artery involvement in Takayasu arteritis is uncommon. Owing to its rarity, the diagnosis is challenging and requires an integrated approach comprising clinical and imaging findings. In order to facilitate early diagnosis of TAK for clinicians, wider use of non-invasive imaging is impacting this.

Case presentation
In this report, we present the imaging characteristics of a rare isolated pulmonary artery involvement in Takayasu arteritis. Pulmonary computed tomography angiography revealed only limited thick walls of both proximal pulmonary artery and stenosis of pulmonary artery lumen, and the other large blood vessels were not involved. The patient undergone pulmonary endarterectomy and pulmonary angioplasty. Then, approximately one month afterward, she passed away due to heart failure.

Conclusions
Imaging examination is the main basis for diagnosing this disease. This impression might improve disease awareness among doctors and progress in diagnosis.
Repetitive peripheral magnetic stimulation for improving upper limb function in post-stroke hemiparesis
Authors: Fawaz Shereen Ismail , Izumi Shin-Ichi , Zaki Amr Saeed , Eldiasty Shaimaa Elmetwally , Saadawy Amr , Saber Heba Gamal eldin , Gadallah May Farouk , Labib Hossam Salaheldin 
Published: October 2023

Abstract
Background
Stroke is one of the leading causes of disability worldwide, with hand and arm weakness, affecting the patients’ daily activities and quality of life. Recently, repetitive peripheral magnetic stimulation (rPMS) was found to enhance neuroplasticity and motor recovery post-stroke hemiparesis via its deep proprioceptive stimulation and simulation of lost voluntary movement.

Objective
To determine the therapeutic effect of rPMS on the functional improvement of upper limb in patients with hemiparesis following cerebrovascular insult and to compare the effect of therapy in subacute and chronic cases.

Results
Post-rehabilitation program both the Fugl-Meyer-Upper Extremity scale (FM-UE) and Functional Independence Measures (FIM) scale showed highly significant improvement in the active group, compared to controls. Regarding active range of motion (AROM) of the shoulder abductors, triceps, wrist extensors and supinators, significant differences were also found in the active group in comparison to controls. Modified Ashworth scale showed also significant change in the active group. When dividing our patients according to the duration post-stroke, into subacute group (6 weeks to 6 months post-stroke) and chronic group (more than 6-month post-stroke), the subacute group showed significant improvements in the FM-UE scale, and in the AROM of wrist extensors and supinators but not in the chronic group. Ultrasonographic measurements showed a significant decrease in cross sectional area of the control group.

Conclusion
rPMS is potentially effective in improving motor recovery post-stroke, especially in the subacute stage.
Dorsum Sellae as Key Landmark in ETV With Disminished Prepontine Cistern: Technical Note and Case Series
Authors: Guil-Ibáñez José Javier , Parrón-Carreño Tesifón , Gomar-Alba Mario , García-Pérez Fernando , Narro-Donate José María , Masegosa-González José 
Published: October 2023

BACKGROUND AND OBJECTIVES:
One of the key aspects in the surgical technique of endoscopic third ventriculostomy (ETV) is the perforation of the floor of the third ventricle because of the high risk of injuring vital structures located in that region. According to the standard technique, this perforation should be performed in the midline halfway between mammillary bodies and the infundibular recess to avoid damage to the structures. This can be performed without excessive complications when the diameter of the prepontine cistern is wide. However, in situations where the diameter is reduced (defined in the literature as having a prepontine interval [PPI] ≤1 mm), the probability of complications increases exponentially.
In this article, we propose using dorsum sellae as a key point to safely perform ETV in patients with a decreased PPI, guiding the trajectory and its marking using neuronavigation.

METHODS:
A review was conducted on the latest 100 ETV procedures performed by our team in the past 5 years. The measurement of the PPI was conducted using archived preoperative MRI imaging studies, specifically between the dorsum sellae and the basilar artery. In cases where the PPI was ≤1 mm and, therefore, the use of the dorsum sellae was applied as a reference point, the technical results and procedural functions were documented.

RESULTS:
In the cohort, 7 patients with a PPI ≤1 mm were identified. In all 7 cases, fenestration of the tuber cinereum was successfully performed without causing vascular damage or associated complications. ETV was successful in 6 patients, with only one experiencing ETV failure necessitating the placement of a ventriculoperitoneal shunt.

CONCLUSION:
The utilization of the dorsum sellae as a reference point to perform ETV in reduced PPI constitutes a safe alternative to the classical technique.
Connexin 43 mediated collective cell migration is independent of Golgi orientation
Authors: Sharma Madhav , Mukherjee Suvam , Shaw Archana Kumari , Mondal Anushka , Behera Amrutamaya , Das Jibitesh , Bose Abhishek , Sinha Bidisha , Sarma Jayasri Das 
Published: October 2023

ABSTRACT
Cell migration is vital for multiple physiological functions and is involved in the metastatic dissemination of tumour cells in various cancers. For effective directional migration, cells often reorient their Golgi apparatus and, therefore, the secretory traffic towards the leading edge. However, not much is understood about the regulation of Golgi's reorientation. Herein, we address the role of gap junction protein Connexin 43 (Cx43), which connects cells, allowing the direct exchange of molecules. We utilized HeLa WT cells lacking Cx43 and HeLa 43 cells, stably expressing Cx43, and found that functional Cx43 channels affected Golgi morphology and reduced the reorientation of Golgi during cell migration. Although the migration velocity of the front was reduced in HeLa 43, the front displayed enhanced coherence in movement, implying an augmented collective nature of migration. On BFA treatment, Golgi was dispersed and the high heterogeneity in inter-regional front velocity of HeLa WT cells was reduced to resemble the HeLa 43. HeLa 43 had higher vimentin expression and stronger basal F-actin. Furthermore, non-invasive measurement of basal membrane height fluctuations revealed a lower membrane tension. We, therefore, propose that reorientation of Golgi is not the major determinant of migration in the presence of Cx43, which induces collective-like coherent migration in cells.
Altered binding affinity of SIX1-Q177R correlates with enhanced WNT5A and WNT pathway effector expression in Wilms tumor
Authors: Stevenson Matthew J. , Phanor Sabrina K. , Patel Urvi , Gisselbrecht Stephen S. , Bulyk Martha L. , O'Brien Lori L. 
Published: October 2023

ABSTRACT
Wilms tumors present as an amalgam of varying proportions of tissues located within the developing kidney, one being the nephrogenic blastema comprising multipotent nephron progenitor cells (NPCs). The recurring missense mutation Q177R in NPC transcription factors SIX1 and SIX2 is most correlated with tumors of blastemal histology and is significantly associated with relapse. Yet, the transcriptional regulatory consequences of SIX1/2-Q177R that might promote tumor progression and recurrence have not been investigated extensively. Utilizing multiple Wilms tumor transcriptomic datasets, we identified upregulation of the gene encoding non-canonical WNT ligand WNT5A in addition to other WNT pathway effectors in SIX1/2-Q177R mutant tumors. SIX1 ChIP-seq datasets from Wilms tumors revealed shared binding sites for SIX1/SIX1-Q177R within a promoter of WNT5A and at putative distal cis-regulatory elements (CREs). We demonstrate colocalization of SIX1 and WNT5A in Wilms tumor tissue and utilize in vitro assays that support SIX1 and SIX1-Q177R activation of expression from the WNT5A CREs, as well as enhanced binding affinity within the WNT5A promoter that may promote the differential expression of WNT5A and other WNT pathway effectors associated with SIX1-Q177R tumors.