bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic office hysteroscopy in a tertiary care centre: a retrospective analysis
Authors: Priya N. Mohna , Lele Prasad R. , Bhardwaj Bikram , Naredi Nikita , Rai Gunjan 
Published: January 2025

Background: Office hysteroscopy is the standard technique for evaluation of intrauterine pathologies in abnormal uterine bleeding, infertility, recurrent pregnancy loss, secondary amenorrhea, post-menopausal bleeding. It is easy to perform, well tolerated by patients and has less complication rates with availability of adequate expertise and equipment. Our aim was to assess the role of diagnostic office hysteroscopy in gynaecology in a referral centre.
Methods: A retrospective descriptive study based on hospital records conducted in a government tertiary care centre. Data collected from hospital registers were analysed for common indications, pathology, complications, safety and efficacy of the procedure.
Results: Diagnostic office hysteroscopy done on 1624 patients from January 2018 to January 2023 were analysed. Most common indication was infertility. Common pathology seen were intrauterine adhesions, mullerian anomalies and endometrial polyps. Procedure was successful in 94.4% of patients. No major complication observed in any patient.
Conclusions: Our findings show that office hysteroscopy is effective, feasible, convenient and safe; in diagnosing various intrauterine pathologies and it is well tolerated by patients. There is no requirement for admission or anesthesia.
Narrar o grande branco: palavra e imagem em Noite dentro da noite, de Joca Reiners Terron
Authors: Santini Juliana 
Published: January 2025

Este trabalho realiza uma análise intersemiótica de Noite dentro da noite – uma autobiografia, de Joca Reiners Terron (2017), problematizando como as fotografias que abrem os capítulos do romance atuam na estruturação de uma narrativa descentrada e fragmentária. A hipótese é de que a impossibilidade de fala do personagem e as imagens, rasuradas, colocam a ditadura militar e o trauma histórico e individual sob o signo do que não pode ser dito – seja porque foi apagado, seja porque sua lembrança é insuportável. A leitura aqui apresentada articula a composição da voz em segunda pessoa à problematização do endereçamento na narrativa, questionando de que modo a fragmentação e os brancos da memória evidenciam, no romance, realizações da cultura brasileira contemporânea em que palavra e imagem ligam-se aos não-ditos da História ora pelos impactos subjetivos do desaparecimento político, ora pela impossibilidade de narrar o trauma.
Chinese Muslim Community and Pseudo-Multiculturalism in West Kalimantan, Indonesia
Authors: Ruslan Ismail , Yusriadi Yusriadi , Muttaqin Imron , Hasriyanti Nunik , Shin Chong 
Published: January 2025

This research aims to explore pluralism in West Kalimantan, a paradoxical place in Indonesia, where multi-religious and multi-ethnic people meet. For social researchers, this situation is unique because, on the one hand, West Kalimantan is known as a conflict-prone area in Indonesia. On the other hand, part of its territory, namely the Singkawang area, is called the most tolerant area. Previous researchers assumed this situation was false because conflicts between religious and ethnic groups still occur frequently. However, the communication strategies and social interactions by ethnic groups in this area have rarely been investigated. The role of the Chinese Muslim group is significant in bringing about harmonization between the polarised groups. Chinese Muslims have provided a link that is socially very instrumental in deconstructing the firm ethnic and religious group identities and boundaries. Although they seem to form their own identity, two sides of that identity are related to religious groups and ethnic groups. Their presence thus balances inter-group communications and interactions. In conclusion, theoretically, the presence of the Chinese Muslim community in West Kalimantan breaks the ice of identity and becomes a bridge of communication between communities.
Body positive social media posts: who likes them anyway? The role of internalization and body dissatisfaction in the implicit and explicit bias towards thin-ideal social media posts
Authors: Schein Adél , Tódor Imre , Varga Attila , Koronczai Beatrix , Szőcs Levente Álmos , Nagy Bence , Stankovics Adél , Ágoston Csilla , Buvár Ágnes 
Published: January 2025

Abstract

Previous research demonstrated that body positive social media posts can foster body acceptance. However, the question arises about the susceptibility to body positive images and whether body positive social media posts can compensate for the harmful effects of unattainable beauty ideal internalization. This paper explored the role of internalization and trait body dissatisfaction in the implicit and explicit biases towards thin-ideal social media posts. We conducted two online studies (N = 673) with female participants of different age groups (adults, young adults, adolescents). Data was analyzed with path models. Results showed that in the adult and young adult samples, stronger internalization led to a more negative appreciation of body size, which was associated with a stronger implicit and explicit bias towards body-positive social media posts. However, in the adolescent sample, while internalization was directly positively related to the explicit bias towards thin-ideal posts, it was also positively associated with general body dissatisfaction that, in turn, was negatively related to the explicit bias towards thin-ideal posts. Our findings confirm that females with body image concerns are more susceptible to body positive posts that might compensate for the possible negative effects of internalization among (young) adults but not necessarily among adolescents.
Polyphenols and antioxidant activity of Sulla pallida from mining sites of Morocco: Implication for phytoremediation of heavy metal-contaminated pastures
Authors: YEMLAHI Anass EL , ZERRAD Hamass , Caprioli Giovanni , Acquaticci Laura , Annaz Houssam , ZERROUK Mounir HASSANI , LAGLAOUI Amin , ARAKRAK Abdelhay , BAKKALI Mohammed 
Published: January 2025

Abstract

An on-field survey was conducted to assess the physiological and symbiotic response of Sulla pallida in an abandoned mining site in Northeast Morocco. Results indicated that S. pallida maintained high nodulation and nitrogen efficiency even at late growth stages while accumulating significant levels of Fe, Pb, Zn, and Cu in its aerial parts. Phenol content varied significantly (p<0.05) by phenological stage and plant part, with a high concentration of total extractable polyphenols (7.73 %DM) and tannins (6.97 %DM) recorded during the vegetative stage. Antioxidant activity, as shown by DPPH radical scavenging (IC50 of 0.126 mg/mL), was comparable to the control (BHT, IC50 of 0.110 mg/mL). Anti-lipid peroxidation and ferric reduction potential were highest during flowering, with values outperforming controls in some assays. HPLC-ESI-MS/MS analysis identified 38 compounds, with high levels of 4-Hydroxy benzoic acid, rutin, vanillic, gallic, and p-coumaric acids. These findings highlighted S. pallid as a promising forage species for remediating heavy metal-contaminated pastures and promoting sustainable land use.
Early Evolution of BA.2.86 Sheds Light on the Origins of Highly Divergent SARS-CoV-2 Lineages
Authors: Escalera-Zamudio Marina , Tan Cedric , Dorp Lucy van , Balloux Francois 
Published: January 2025

Abstract

Since the emergence of SARS-CoV-2 in late 2019, highly divergent variants with novel constellations of mutations have periodically emerged to displace previously co-circulating virus lineages. The evolutionary mechanisms behind this process remain unclear, but the prevailing hypothesis supports emergence through single events followed by a rapid accumulation of mutations in individual long-term infections. In July 2023, the Omicron-descending BA.2.86 Variant of Interest (VOI) emerged to outcompete other virus lineages, with descending sublineages still dominating as of December 2024. We use BA.2.86 as a case study to test the hypothesis that highly divergent variants emerge during a single chronic infection. By applying a comprehensive suite of evolutionary analyses to independent high-resolution datasets, we identify a cluster of approximately 100 BA.2 genomes that fall directly ancestral and shorten the branch leading to BA.2.86, carrying some BA.2.86-specific lineage defining mutations. These genomes, sampled from multiple countries and at different timepoints, share a single ancestor dating back to December 2021. Additionally, we detect over 2600 earlier BA.2, BA.2.75, and XBB.1.5 genomes with multiple mutations later associated with BA.2.86. These genomes represent a standing genetic variation that likely facilitated a gradual evolution in the direct ancestry of this variant. We further uncover a complex evolutionary process involving recombination between the BA.2.75 and XBB.1.5 parental lineages. Together, our results challenge the notion of a single emergence event, and instead support a more complex scenario, with BA.2.86 likely having evolved over multiple transmission chains.
Intron retention, a novel method for evaluating the response to ketamine in patients with treatment-resistant depression
Authors: okada norihiro , Oshima Kenshiro , Maruko Akiko , Kobayashi Yoshinori 
Published: January 2025

Abstract

In response to a state of stress, the body attempts to maintain protein homeostasis through intron retention (IR). In other words, by examining which genes undergo IR under stress (i.e., IR genes), it is possible to analyze what type of stress the body is experiencing (Okada et al. 2024 Front.Psychiatry). Using this principle, we re-analyzed the RNA-seq data of non-responders and responders to ketamine, a drug with specific efficacy for depression, published by Cathomas et al. (2022), and examined IR genes to investigate what the substance of non-responders is. It was found that non-responders were individuals in a state of elevated viral infection. Several IR genes associated with viral infection were restored to a healthy state by ketamine regardless of whether an individual was a non-responder or a responder, so it is more reasonable to interpret that the non-responders are not individuals for whom ketamine does not work, but rather that the effects of ketamine do not catch up due to the extremely elevated inflammatory state of the non-responders. Furthermore, there is one transcriptomic outlier in the non-responders who shows an extreme increase in viral infection, but even if such an individual is excluded from the analysis, the above conclusions from the IR gene analysis remain essentially unchanged, whereas the DEG data were largely changed after this treatment. This further supports our previous claim that IR genes, but not DEGs, may be useful as markers of depression. This study is the first to describe the molecular basis of the difference between non-responders and responders.
WITHDRAWN: Zinc deficiency triggers ribosome remodeling to reverse biofilm azole resistance in Candida albicans
Authors: Wang Yingzhe , Ye Shigan , Deng Yuan , Huang Yingting , Zhu Xiaoliang 
Published: January 2025

Abstract

Biofilm formation is a key cause of high azole resistance and prolonged infection in Candida albicans. Zinc ion is involved in the regulation of biofilm formation and azole resistance in C. albicans, but its specific role remains unknown. We assessed the alteration of azole resistance in C. albicans biofilm in vivo and in vitro by inducing zinc deficiency, and found that zinc deficiency inhibits biofilm formation, reduces the activity of efflux pumps, enhances cellular metabolism levels, reduces in vivo pathogenicity, and ultimately reverses azole resistance. RNA-seq showed that zinc deficiency promotes cell survival under zinc deficiency by triggering intracellular ribosome remodeling, and downregulates the expression of genes related to biofilm core transcription factors and efflux pumps and upregulates the expression of genes related to glucose metabolism. This study provides new insights into the treatment of clinical C. albicans infections, particularly biofilm-associated highly resistant and persistent infections, by targeting zinc.
Study on the mechanism of action of Jingfang mixture against carrageenan-induced thrombosis in rats based on the gut-liver axis
Authors: Ni Wenting , Wang Yunyu , Li Shirong , Cao Qingyu , Sun Chenghong , Yao Jingchun , Zhang Guimin 
Published: January 2025

Abstract

The incidence of thrombosis-related diseases is increasing globally, and it is often treated by mechanical thrombectomy, intravenous thrombolysis and antiplatelet, but it is easy to lead to drug resistance and bleeding complications, which limits the development of its treatment. The gut-liver axis represents a complex two-way communication system, in which intestinal microorganisms not only affect intestinal immunity and intestinal function, but also regulate the coagulation function of the liver. Jingfang Mixture (JF) is a Traditional Chinese Medicine (TCM) that have the function of antithrombotic, but the mechanism is still unclear. This study is aimed to explore the regulatory effect and mechanism of JF on thrombosis rats through the gut-liver axis through a carrageenan-induced rat model. The results showed that after JF treatment, the degree of black tail in rats with thrombosis was significantly reduced, the pathological condition of the tail was improved, the four coagulation parameters and the levels of coagulation factors were adjusted closely to the normal levels, and the levels of liver and intestinal inflammatory factors were significantly reduced. In vitro experiments proved JF had little effect on platelet aggregation, release, and coagulation. In our study, JF can significantly elevate the levels of intestinal tight junction proteins and improve intestinal flora disorders, alleviate the oxidative stress level in the liver, and improve the short-chain fatty acid levels in the feces of rats with thrombosis. Of note, the relative abundances of Clostridia_UCG-014, Ruminococcaceae, Eggerthellaceae, Monoglobaceae and Rikenellaceae were significantly were decreased after JFM intervention. Proteomic results showed that the antithrombotic effect of JF may be related to the coagulation and complement cascade signaling pathway. The gene and protein expression of FGA, FGB, FGG and C3 were elevated after JF treatment, respectively. Finally, we further confirmed the therapeutic effect through fecal microbiota transplantation (FMT) experiments, that the crosstalk of the gut-liver axis contributed to the improvement of thrombosis after JF treatment. In summary, this study took the gut-liver axis as the breakthrough point and coupled with 16S rDNA sequencing, non-targeted metabolomics, TMT-based quantitative proteomics and related comprehensive analysis methods, showing that JF can improve the intestinal flora and immune system disorder, as well as reduce the inflammatory damage of intestinal and liver in carrageenan-induced thrombosis rats, and its protective effect may be related to the complement and coagulation cascade signaling pathway. This study accurately explains the multi-target and multi-pathway mechanism of JF in treating thrombosis-related diseases, which can provide reference for further clinical application.
Bridging the Medication Adherence Gap from Therapeutic Drug Monitoring: A Bayesian approach for Anti-Seizure Medications
Authors: Jiao Zheng , Liu Xiao-Qin , Li Zi-Ran , Lin Wei-Wei , Wang Juan , Gu Fu-Qing , Ding Junjie 
Published: January 2025

Abstract

Background: Adherence to antiseizure medications (ASMs) is crucial for the success of treatment. However, current recommendations for assessing medication adherence through therapeutic drug monitoring (TDM) may overlook individual patient characteristics, potentially leading to misjudgments. This study aims to evaluate the capability of a Bayesian approach in assessing adherence for 14 ASMs using TDM.
Method: A Bayesian framework incorporating population pharmacokinetics was used to assess adherence using TDM data. Additionally, the impact of patient characteristics, concomitant medications, sampling times, and prior adherence probability was examined.
Results: With essential patient information, such as age, weight, and scheduled dosing regimen, the Bayesian approach effectively assessed recent adherence for all investigated ASMs. The concentration thresholds varied by ASM and were influenced by patients' characteristics. To facilitate individual adherence evaluations, a web-based dashboard was developed.
Conclusion: The integration of Bayesian methods with pharmacokinetics significantly enhances the reliability of TDM in assessing adherence to ASMs.
IMRC-Exo Alleviates Limb Injury Induced by Deinagkistrodon acutus Snakebite in Rabbits Through GSDME-Dependent Pyroptosis Inhibition
Authors: Wu Haohao , Xie Lutao , Du Wang , Lai Linjie , Shangguan Peixin , Wu Xingzhen , Xu Jiefeng , Lan Pin 
Published: January 2025

Abstract

Background
Inflammation plays a critical role in the pathogenesis of limb injury caused by Deinagkistrodon acutus snakebite. Investigating its regulatory mechanisms and intervention strategies may uncover effective therapeutic approaches for this condition. Recent studies have identified pyroptosis as a key pathway exacerbating target organ damage by amplifying inflammatory responses. Immune and Matrix-Regulatory Cells (IMRC), a novel type of mesenchymal stem cell, and their derived exosomes (Exo) that have shown potential in mitigating inflammation-mediated tissue damage by suppressing pyroptosis. This study aimed to evaluate whether IMRC-Exo could alleviate Deinagkistrodon acutus venom-induced limb injury by inhibiting pyroptosis-mediated inflammation in rabbits.
Methods
Eighteen healthy male New Zealand white rabbits were randomly assigned to the Sham, Model, and IMRC-Exo groups. The Model group was established by intramuscular injection of Deinagkistrodon acutus venom (1.5 mg/kg), followed by intravenous infusion of anti-D. acutus venom serum (80 U/kg) after 2 hours. The IMRC-Exo group received IMRC-Exo treatment (7.5 × 10^10 particles) after model establishment. Within 24 hours post-modeling, the left thigh circumference, serum creatine kinase (CK), and myoglobin (Mb) levels were dynamically assessed. Animals were euthanized to collect muscle tissues for histopathological examination, apoptosis analysis, inflammatory cytokine quantification (HMGB1, IL-1β, IL-18), and pyroptosis-related protein detection, including caspase-3, cleaved caspase-3, gasdermin E (GSDME), and N-terminal GSDME (N-GSDME).
Results
Compared with the Sham group, both venom-injected groups exhibited a significant increase in left thigh circumference, elevated serum CK and Mb levels, and aggravated histopathological damage and apoptosis in muscle tissues. However, the IMRC-Exo group showed significantly reduced limb circumference, decreased muscle injury markers, and attenuated tissue damage compared with the Model group. Additionally, venom injection significantly increased HMGB1, IL-1β, IL-18 levels, and the expression of caspase-3, cleaved caspase-3, GSDME, and N-GSDME in muscle tissues of the Model and IMRC-Exo groups compared to the Sham group. Notably, these inflammatory cytokine levels and pyroptosis-related protein expressions were significantly lower in the IMRC-Exo group than in the Model group.
Conclusion
IMRC-Exo effectively alleviates limb wound damage induced by Deinagkistrodon acutus snakebite in rabbits. Its protective mechanism may involve the inhibition of GSDME-dependent pyroptosis-mediated inflammatory injury.
The Omics Molecule Extractor: A web application for the selection of potential biomarker panels
Authors: Lange Emanuel , Schallert Kay , Schwerdt Johannes , Ghosh Susmita , Hentschel Andreas , Reinders Yvonne , Heyer Robert 
Published: January 2025

Abstract

Selecting molecule panels that are applicable to classify the health state of patients is a common task in omics data analysis. Existing software for molecule selection lacks features to select molecule panels from large datasets, requires programming experience, or lacks user-friendly interfaces. We present the Omics Molecule Extractor (OMEx) an open-source web application providing a user-friendly workflow for selecting molecules and molecule panels for sample classification from large datasets. OMEx’s user interface provides interactive visualization for exploring input data and analysis results. The feature selection strategy underlying the algorithm is based on machine learning and has not been available in any software with user interface. Extensive testing using synthetic datasets with known ground truth showed that the algorithm discovers group-separating molecules with high precision. Additionally, OMEx was tested on five real-world omics datasets demonstrating high reproducibility and overlap with reported molecules from other feature selection methods, while also reporting alternative molecules of interest. OMEx is freely available at https://mdoa-tools.bi.denbi.de/omex/home.
A Study of Gender-Specific Learning Patterns in Online Medical Education
Authors: Moranges Maëlle , Ibrahim Noha , Amer-Yahia Sihem , Palombi Olivier 
Published: January 2025

Abstract

This study explores gender-specific learning patterns among medical students using an online educational tool utilized at the national level in France. Despite increasing numbers of women in medical school admissions, persistent disparities in professional medical careers raise concerns that such inequalities may already be present during university training. In this study, we analyze data from all fourth-year medical students in France for the academic years 2022-2023 and 2023-2024. We employ a Subgroup Discovery algorithm to identify patterns in online engagement and performance within these two national cohorts, focusing on gender as the key demographic variable. Our analysis revealed significant gender differences, with a proportion of male students displaying higher levels of engagement and academic performance. In contrast, female students were more likely to disengage from interactive components of the platform or to perform poorly or averagely in some medical specialties. We hypothesize that these differences may be attributed to the nature of the evaluations, which are predominantly in multiple-choice question format, potentially favoring male students. Additionally, stress and psychological distress, which are known to affect female students, may further exacerbate these performance disparities. These findings underscore the need for targeted interventions in medical education to address early gender disparities, promoting a more equitable learning environment for all students.
Rainfall patterns and their association with flood fatalities across diverse Euro-Mediterranean regions over 41 years
Authors: Papagiannaki Katerina , Lagouvardos Konstantinos , Kotroni Vassiliki , Diakakis Michalis , Kyriakou Petros 
Published: January 2025

Abstract

Flood fatalities remain a critical challenge in the Euro-Mediterranean region. This study analyses 2,245 fatalities from 11 territories (1980–2020) using the FFEM-DB and the global MSWEP rainfall dataset. Extreme rainfall is shown to be a significant risk factor, with fatal floods in many regions associated with rainfall exceeding the 99th percentile of daily values over 41 years. However, in some territories, fatalities also occurred under less severe rainfall, reflecting regional differences in exposure and vulnerability. In the South EU-Mediterranean, deadly floods are triggered mainly by higher rainfall than in the Central EU. Autumn is the most hazardous season, though summer floods in the South EU-Mediterranean demonstrate heightened exposure and impact severity. The findings are discussed in light of the climatology, geomorphology, and societal vulnerabilities, highlighting the importance of tailored management strategies to address regional disparities, particularly as climate change intensifies rainfall patterns across the Euro-Mediterranean region.