bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Enzyme-Free Approach for Precursor microRNA Cloning and Expression
Authors: Hashemabadi Mohammad , Amandadi Mojdeh , Shaban Mojtaba , Sasan hosseinali 
Published: December 2023

Abstract
Background: Traditional cloning methods heavily depend on enzymatic digestion for generating sticky ends in DNA inserts, presenting challenges in efficiency and cost-effectiveness. MicroRNAs play crucial roles in biological processes and are integral to disease studies. Cloning microRNA precursors involves directional binding, necessitating meticulous techniques. Existing methods require multiple enzymes and suffer from limitations, urging the need for innovative, streamlined approaches.Methods: This study introduces a revolutionary technique eliminating the reliance on restriction enzymes for cloning pre-microRNA genes. Strategic manipulation of the expression vector's sequence and design of tailored primers enable consecutive PCR reactions, producing pre-microRNA sequences with compatible sticky ends. The engineered vector, incorporating BbsI recognition sites, undergoes a simplified cloning process, validated through colony PCR and sequencing. HEK293 cells transfected with the construct affirm method robustness, demonstrating microRNA-21 expression via real-time PCR.Results: The method yields pre-microRNA sequences with compatible ends for the expression vector, eliminating costly and time-consuming enzymatic digestion. Optimizing BbsI enzyme usage reduces the risk of re-ligation. The approach significantly accelerates cloning, completing the process in approximately 4 hours with high precision. Real-time PCR confirms a 12.5-fold increase in microRNA-21 expression compared to controls, validating successful transfection.Conclusion: This study presents an innovative, cost-effective, and rapid cloning method for microRNA precursor genes. By bypassing the limitations of traditional techniques, this approach accelerates molecular biology research, offering a dependable protocol for various pre-microRNAs and expression vectors. The method's simplicity, efficiency, and reliability mark a significant advancement, fostering progress in the field of molecular biology.
Association between health literacy and suicide-related behaviors in middle school students: The mediating role of depressive symptoms
Authors: Zhang Ronghuinan , Liu Aini , Luo Yu , Wang Hong 
Published: December 2023

Abstract

Background
The health literacy level is closely related to mental health. Depressive symptoms are one of the most common mental health problems and are strongly associated with suicide-related behaviors among middle school students. The purpose of this study was to examine the mediating role of depressive symptoms in the relationship between health literacy and suicide-related behaviors in middle school students.
Method
A total of 7182 middle school students were included in this analysis. A paper-based self-administered questionnaire was used to collect data in May 2023. Multiple linear regression and multivariate logistic regression models were applied to analyse the association between health literacy, depressive symptoms and suicide-related behaviors. The Monte Carlo method was used to determine whether depressive symptoms mediated the relationship between health literacy and suicide-related behaviors.
Results
The prevalence of suicidal ideation, planning, and attempts in the past 12 months among middle school students was 26.62%, 9.27%, and 4.55%, respectively. Health literacy levels exhibited a significant and negative correlation with depressive symptoms, suicidal ideation, suicide planning, and suicide attempts, whereas depressive symptoms was correlated significantly and positively with three suicide-related behaviors. Mediation analysis showed that depressive symptoms mediated the relationship between health literacy and suicidal ideation, suicide planning, and suicide attempts, with mediating effect sizes of 47.5%, 43.0% and 41.8%, respectively.
Conclusion
Depressive and anxiety symptoms mediate the relationship between health literacy and suicide-related behaviors. Comprehensive health literacy improvement programs may be useful in preventing depression and suicide-related behaviors.
Current practice of intracranial pressure monitoring in children with severe traumatic brain injury - a nationwide prospective surveillance study in Germany
Authors: Brensing Pia Sophie , Greve Sandra , Hojeij Rayan , Dammann Philipp , Felderhoff-Müser Ursula , Dohna-Schwake Christian , Bruns Nora 
Published: December 2023

Abstract
Background
For management of severe traumatic brain injuries (sTBI) in children, the overall level of evidence to guide diagnostic and therapeutic procedures is low. Since 2016, international guidelines have subsequently suggested invasive intracranial pressure (ICP) monitoring in patients with initial Glasgow Coma Scale (GCS) ≤ 8. In Germany, ICP monitoring was an individual case decision from 2011 until the 2022 update of the German pediatric TBI guideline. The aim of this study was to evaluate current clinical practice of invasive ICP monitoring in Germany in children < 10 years with respect to guideline recommendations.
Methods
Anonymized clinical data on sTBI cases < 10 years of age were collected in a nationwide prospective surveillance study via the German Pediatric Surveillance Unit ESPED from July 2019 until June 2022. Inclusion criteria for the surveillance study were sTBI (initial GCS ≤ 8) or neurosurgery following TBI. For this analysis, only cases with GCS ≤ 8 were subject to the present analysis. Descriptive analyses were performed to assess the proportion of ICP monitored patients and describe the cohort.
Results
Out of 217 reported cases, 102 cases met the inclusion criteria and thus qualified for ICP monitoring. Of these, 37 (36%) received ICP monitoring. Monitored patients were older, had lower median GCS values at presentation (4 vs. 5), higher mortality (32% vs. 22%), and were more frequently diagnosed with cerebral edema (68% vs. 37%).
Conclusion
In children < 10 years with sTBI, the present clinical management regarding ICP monitoring deviates from the current German national and international guidelines. The reasons remain unclear, with the low level of evidence in the field of ICP monitoring and the recency of changes in guideline recommendations as potential contributors. Prospective interventional studies should elucidate the benefit of ICP monitoring and ICP directed therapies to provide evidence-based recommendations on ICP monitoring.
Trial registration
German Clinical Trials Registry, DRKS00022807. Registered 11 August 2020 - Retrospectively registered, https://drks.de/search/de/trial/DRKS00022807
Phenotypic and genotypic characterization of awns in elite U.S. rice breeding germplasm
Authors: Borjas Anna H. , Famoso Adam N. , Angira Brijesh , De Guzman Christian , Oard James H. 
Published: December 2023

Abstract
Awns are needle or bristle-like structures that grow at the tip of the lemma in rice grains. In cultivated rice, awnless material is preferred for agricultural and processing practices. Most cultivated rice produces no awns or very short awns, however, the causal genetic factors responsible for the loss of awns in cultivated rice remain largely unknown. Most studies conducted to investigate the genetic factors responsible for awn formation focused on plant material derived from crosses between awnless and wild awned parents. In this study, genetic analysis of awns was carried out using a breeding population developed from U.S. elite conventional and Provisia™ awnless parents. Before the use of indica germplasm and the Provisia™ technology, awns were not typically observed in the elite U.S. breeding material, where tropical japonica is predominant. We hypothesized that the presence of awns in populations derived from crosses with Provisia™ material was due to its indica background. We mapped a 1557 Kb awn-related region on chromosome 3 that appears to be a major source of awn formation when Provisia™ material (indica-type) is used for crossing with U.S. elite varieties. Two single nucleotide polymorphism (SNP) markers, SNP261 and SNP1251 explained 26% and 28% of the phenotypic variation in awn formation and elongation. Contingency analysis of F2, F2:3, and F3:4 generations showed that the awnless, short-awned, and low percentage of awns per panicle phenotype is explained by the presence of japonica alleles. When used together, these markers help differentiate awnless material.
Sports Recommender Systems: Overview and Research Issues
Authors: Felfernig Alexander , Wundara Manfred , Tran Thi Ngoc Trang , Le Viet-Man , Lubos Sebastian , Polat-Erdeniz Seda 
Published: December 2023

Abstract
Sports recommender systems receive an increasing attention due to their potential of fostering healthy living, improving personal well-being, and increasing performances in sport. These systems support people in sports, for example, by the recommendation of healthy and performance-boosting food items, the recommendation of training practices, talent and team recommendation, and the recommendation of specific tactics in competitions. With applications in the virtual world, for example, the recommendation of maps or opponents in e-sports, these systems already transcend conventional sports scenarios where physical presence is needed. On the basis of different working examples, we present an overview of sports recommender systems applications and techniques. Overall, we analyze the related state-of-the-art and discuss open research issues.
Establishment of a real-time recombinase-aided isothermal amplification (RT-RAA) assay for the rapid detection of bovine respiratory syncytial virus
Authors: Hou Guanxin , Sun Xinyi , An Rui , Zhang Chunxiao , Wang Lili , Li Hong , Zhu Siping , Shi Quimei , Zhang Zhiqiang 
Published: December 2023

Abstract
Background Bovine respiratory syncytial virus (BRSV) is a major cause of bovine respiratory disease, resulting in significant losses to the cattle industry. For rapid detection of BRSV, reverse transcription recombinase polymerase amplification assays targeting the F gene were developed by integrating the fluorescence detection platform (RT-RAA).Results The developed RT-RAA assays allowed the exponential amplification of the target fragment in 20 min at a constant temperature of 39°C. The RT-RAA assays also showed good specificity for BRSV, with no cross-reactions with Infectious Bovine Rhinotracheitis Virus (IBRV), Bovine Parainfluenza Virus Type 3 (BPIV3), Bovine Viral Diarrhea Virus (BVDV) and Bovine Coronavirus (BCoV). With the standard RNA of BRSV serving as a template, the limit of detection for RT-RAA was 5 × 102 copies per reaction. Forty clinical samples collected from cattle with respiratory disease were tested, and the positive rate was 7.5% (3/40), consistent with results using the conventional PCR method reported previously.Conclusion An RT-RAA assay for BRSV detection was established in this study. The method is specific and sensitive and can be completed within 20 min at 39℃. These results ascertain that the developed RT-RAA assays are effective diagnostic tools for rapidly detecting BRSV in resource-limited settings, which may be applied for clinical detection of BRSV.
High-risk factors and predictive models for hemorrhagic chronic radiation proctitis
Authors: Liao ZhongLi , Hu XiaoGang , Hu LiuLing , Yang Jian 
Published: December 2023

Abstract
Background
Hemorrhagic chronic radiation proctitis (CRP) is the most common and stubborn complication after pelvic radiation therapy. Understanding the high-risk factors, exploring a method to predict it, and then formulating a reasonable radiotherapy plan are the keys to preventing hemorrhagic CRP. The aim of this study was to retrospectively identify potential risk factors for hemorrhagic CRP and establish a nomogram to predict the onset of hemorrhagic CRP.
Methods
In this retrospective study, we considered patients who received pelvic radiotherapy for cervical carcinoma from March 2014 to December 2021 at Chongqing University Cancer Hospital (Chongqing, China). Logistic regression analysis was performed to determine the factors and then a nomogram model was established. To evaluate the performance of the model, metrics such as the area under the receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis were used.
Results
A total of 221 patients were included.125 patients diagnosed with hemorrhagic CRP by colonoscopy. The median time of diagnosis of hemorrhagic CRP was 14.45 months after pelvic radiotherapy. Age (≥ 54 years old), weight (< 52 kg) and total radiation dose (≥ 72 Gy) were identified as risk factors and then a nomogram was established to predict the onset of hemorrhagic CRP. The area under the ROC curve (AUC) values of the nomogram were 0.741 and 0.74 in the training and the validation cohort, respectively. To investigate the clinical utility of the model,decision curves and clinical impact curves were further performed. The results showed that the model was beneficial over a wide probability range from 0.25 to 0.85. and 0.74 in the training and the validation set, respectively.
Conclusion
In this study, we constructed and developed a nomogram for hemorrhagic CRP. The calibration curves, ROC curve analysis, and decision curves showed that the nomogram was reliable for clinical application. It may provide some evidence for radiologist to make a reasonable radiotherapy plan to preventing hemorrhagic CRP.
Trial registration
retrospectively registered.
Medium-Long Term Electricity Load Forecasting Based on NSNP Systems and Attention Mechanism
Authors: Guo Lin , Wang Jun , Peng Hong , Gao Yunzhu 
Published: December 2023

Abstract
Accurate load forecasting can provide important information support for intelligent operation of power systems, it can assist the power grid to deploy production plans in advance to uphold the equilibrium between the supply and demand for electrical power, or plan investment strategies based on the results of the forecast. Nonlinear Spiking Neural P (NSNP) system belongs to a category of computational systems with distributed, parallel and non-deterministic characteristics that have the analytical skill to solve nonlinear problems. Aiming at the temporal characteristics and complex nonlinear characteristics of electrical load data, this paper proposes a new Medium-Long Term Load Forecast model LF-ASNP based on NSNP system and attention mechanism, which can accurately analyze the characteristics of historical load data and forecast the electrical load. In this paper, the LF-ASNP model is validated in several benchmark datasets, and the analysis of the experimental results fully demonstrates that the model can forecast the power load effectively and reliably.
Iris Recognition: An analysis using LBP, SVC and Distance metrics
Authors: Y Mahesha 
Published: December 2023

Abstract
In this paper, experiment has been conducted to find the optimum iris recognition system between the combinations Local Binary Pattern (LBP) and Distance metric, and LBP and Linear Support Vector Classifier (SVC). First, experiment has been conducted using LBP and different distance metrics. For each of the distance metric, the FAR, FRR and accuracy have been calculated for different threshold values. From the obtained result, it has been found that cityblock distance gives better accuracy compared to remaining distance metrics and the accuracy obtained is 65.93% on CASIA iris dataset. Secondly, iris recognition has been carried out using Local Binary Pattern (LBP) and Linear Support Vector Classifier (SVC). The combination of LBP and Linear SVC is giving an accuracy of 91.83% on CASIA iris dataset.
Estimation of daily suspended sediment concentration in the Ca River Basin using a sediment rating curve, multiple regression, and long short-term memory model
Authors: Pham Van Chien , Le Hien , Van Chin Le 
Published: December 2023

Abstract

This study presents a sediment rating curve (SRC), multiple regression (MR), and long short-term memory (LSTM) model for estimating daily suspended sediment concentration (SSC). The data of daily SSC at Yen Thuong and daily flow at five locations in the Ca River Basin, Vietnam are used to demonstrate multiple approaches. Using the daily flow and SSC data in the period from 2009 to 2019, appropriate coefficients in each method are identified carefully using five popular criteria. The results showed that SRC and MR approaches reproduced acceptably the observed values, with the values of RMSE, MAE, and ME of daily SSC being less than 5% of daily SSC magnitude observed at the station, while NSE ranges from 0.47 to 0.63 and r coefficient varies between 0.69 and 0.80. The LSTM model represented the observed values of daily SSC very well. The values of two dimensionless criteria are greater than 0.94 and its values of three-dimensional criteria are smaller than 2.0% of the observed magnitude of daily SSC in both training and validation steps. The LSTM model is found to be the best among the three investigated approaches. Then, the model is applied to estimate daily SSC values for the period from 1969 to 2008 and the year 2020.
A Bioinformatics Study of the Influenza H5N1 Virus that Infects Wild Fowl and Poultry and, Potentially, Humans
Authors: Polanco Carlos , Uversky Vladimir N. , Huberman Alberto , Hernandez-Lemus Enrique , Martínez-Garcia Mireya , Hernández Claudia Pimentel , Rios Castro Martha , Buhse Thomas , Alarcon Gilberto Vargas , González Jorge Alberto Castañón , Díaz González Juan Luciano , Sanchez Díaz Mauricio Missael , Oliva Erika Jeannette López , Gomez Francisco J. Roldan , Luna Brayans Becerra 
Published: December 2023

Introduction:
More than sixteen countries are infected with the highly pathogenic avian
influenza A-H5N1 virus. As the virus circles the world, it has led to the slaughter of millions of poultry
birds as well as the infection of farmed mink in Europe and among seals and grizzly bears on the
Northern coast of America; some infected mammals exhibited neurological symptoms like disorientation
and blindness, which marks the first large H5N1 outbreak potentially driven by mammal-tomammal
transmission that could be poised to spillover into humans its mortality rate in humans exceeds
50%. The virus pandemic potential is continuously monitored to characterize further and
analyze its zoonotic potential (PAHO/WHO, March 2023).

Objective:
To gain comprehension of the envelope glycoproteins that express H5N1 influenza (hemagglutinin
and neuraminidase proteins), computational studies were carried out.

Methods:
Calculating each sequence's Protein Intrinsic Disorder Predisposition (PIDP) and Polarity
Index Method Profile 2.0 v (PIM 2.0 v) required the employment of multiple computer algorithms.

Results::
The analysis of the PIM 2.0 v and PIDP profiles revealed specific patterns within the envelope
glycoproteins (neuraminidase and hemagglutinin) of diverse strains of the H5N1 influenza
virus. These patterns made it possible to identify structural and morphological similarities.

Conclusions:
Using the PIM 2.0 v profile, our computational programs were able to identify the influenza
H5N1 virus envelope glycoproteins (hemagglutinin and neuraminidase strains). This study
contributes to a better comprehension of this emerging virus.
A nyírbátori minorita templom építéstörténete és photo scanner felmérése
Authors: Turi Olivér 
Published: December 2023

The medieval Minorite church of Nyírbátor suffered serious damages and became abandoned at the end of the 17th century. In the 18th century, the baroque reconstruction of the church began, and later a second period of Baroque modifications took place. After this, the church remained untouched for a long time. Then, in the 20th century once again renovations were deemed necessary, because of its poor condition at the time. During the 19th and until the end of the 20th century, several restoration works and archaeological excavations took place, which focused on certain aspects of the church. In this article, I would like to present a summary of the notes concerning the construction history of the church and the results and methods of the photo scanner survey I carried out.
Comparative analysis of the levels of thyroid-stimulating hormone, glycated hemoglobin and indicators of lipid metabolism in women of Lviv and Kyiv regions
Authors: Moskva Kh.A. , Kikhtyak O.P. 
Published: December 2023

Background. The prevalence of thyroid diseases, including endemic goiter and autoimmune disorders, has been consi­dered by scientists for more than a century as a cause for reducing the working capacity of the population and also affects the cognitive ability of future generations. Geographically, Lviv region is in an endemic iodine deficiency zone, while Kyiv region suffered negative radiation exposure as a result of the accident at the Chernobyl Nuclear Power Plant. Since both factors affect the state of the thyroid system, the study on the levels of thyroid hormones among women in the specified areas makes it possible to identify the risks of thyroid pathologies and to develop a strategy for their prevention. The purpose of the study: to find out and analyze the levels of thyroid-stimulating hormone (TSH), glycated hemoglobin and indicators of lipid metabolism in women of Lviv and Kyiv regions. Materials and methods. One hundred and fifty women were examined, their average age was 48 ± 7 years. The criterion for inclusion in the study were: absence of diabetes or treatment for thyroid pathologies, as well as other serious diseases. The survey was conducted once, in July-August 2022. The level of pituitary TSH, antibodies to thyroid peroxidase (TPO-Ab), glycated hemoglobin, total cholesterol, low-density lipoprotein was evaluated. Results. In examined women of the Kyiv region, the average TSH was 2.21 ± 0.30 mIU/l, while in those from the Lviv region it was within 2.42 ± 0.17 mIU/l (p > 0.05). A significant difference was found between the average values of TPO-Ab, some women had high levels of antibodies without manifestations of hypothyroidism and clinical complaints. The average value of TPO-Ab in patients from the Kyiv region was 81.21 ± 19.41 IU/ml and in women from the Lviv region it was 38.41 ± 5.97 IU/ml (р < 0,05). When analyzing carbohydrate metabolism, no significant changes were found between the levels of glycated hemoglobin in the examined women of Kyiv and Lviv regions, 5.81 ± 0.09 % and 5.66 ± 0.04 %, respectively (р > 0.05). Some women showed a slight increase in glycated hemoglobin over 5.6 %, which did not reach the level of 6.5 %. This group of examinees were at risk of developing type 2 diabetes and is characterized by the term “prediabetes”. Regarding lipid metabolism, we found no significant changes in the level of total cholesterol and low-density lipoprotein. Conclusions. The conducted observation revealed differences in TSH, TPO-Ab indicators in healthy women living in Lviv and Kyiv regions. We can assume that the obtained results of Lviv region reflect the problems of the endemic zone of the Western regions of Ukraine. In turn, significantly higher levels of TPO-Ab among residents of Kyiv region are most likely a consequence of the accident at the Chernobyl Nuclear Power Plant, which can still affect the health of the population of this region.