bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiac interoception in Anorexia Nervosa: A resting‐state heartbeat‐evoked potential study
Authors: Cambi Susanne , Solcà Marco , Micali Nadia , Berchio Cristina 
Published: November 2023

AbstractObjectiveA deficit in interoception ‐ the ability to perceive, interpret and integrate afferent signals about the physiological state of the body ‐ has been shown in Anorexia Nervosa (AN), and linked to altered hunger sensations, body dysmorphia, and abnormal emotional awareness.The present high‐density electroencephalography (hdEEG) study aims to assess cardiac interoception in AN and to investigate its neural correlates, using an objective neurophysiological measure.MethodHeartbeat‐evoked potentials (HEPs) were computed from 5 min of resting‐state EEG and electrocardiogram (ECG) data and compared between individuals with AN (N = 22) and healthy controls (HC) (N = 19) with waveform, topographic, and source imaging analyses.ResultsDifferences in the cortical representation of heartbeats were present between AN and HC at a time window of 332–348 ms after the ECG R‐peak. Source imaging analyses revealed a right‐sided hypoactivation in AN of brain regions linked to interoceptive processing, such as the anterior cingulate and orbitofrontal areas.ConclusionsTo the best of our knowledge, this is the first study using hdEEG to localise the underlying sources of HEPs in AN. Results point to altered interoceptive processing during resting‐state in AN. As our participants had a short duration of illness, this might not be the consequence of prolonged starvation. Interventions targeted at interoception could provide an additional tool to facilitate recovery.
Towards a software‐defined networking model for consumer‐centric content delivery network for IoT
Authors: Bhardwaj Shikhar , Harit Sandeep , Yadav Anil 
Published: November 2023

AbstractEvery entity must communicate seamlessly in an interconnected network. Personalised delivery of information in an open private network(s) among consumers should be transparent while maintaining privacy. In information‐centric content delivery networks (ICDN) or data‐centric content delivery networks (DCDN), data delivery relies heavily on sharing data with third‐party entities, resulting in a privacy breach. To overcome these challenges, this article proposes a novel communication model with a consumer‐centric content delivery network (CCDN) approach for an internet‐of‐things (IoT) network that deals in personalised information delivery with users' identity and location privacy. The article derives motivation from a use case‐based analysis of the proposed communication model (CCDN) by incorporating the latest technologies like software‐defined networks (SDN) and content delivery network (CDN) into IoT Networks to achieve a higher level of personalisation while maintaining user privacy. CCDN leverages SDN‐based CDN for IoT networks in the designed use case that strictly adheres to user‐defined preferences/demands. The article suggests an environment with a possible topology implementation and describes the elements that can help achieve it. The article investigates the need for personalisation and how it is contrary to maintaining user privacy. The article also contributes a survey of the underlying technologies while linking them to IoT.
Exogenous citric acid, salicylic acid, and putrescine treatments preserve the postharvest quality and physicochemical properties of broccoli (Brassica oleracea L. var. italica) during cold storage
Authors: Kibar Hakan , Kibar Beyhan , Turfan Nezahat 
Published: November 2023

AbstractTo extend the postharvest storage life of broccoli samples (Brassica oleracea L. var. italica), an exogenous application of citric acid (CA), salicylic acid (SA), and putrescine (PUT) was tested in multiple combinations (0.5 and 1 mM) at 4 ± 0.5°C and 90 ± 5% relative humidity (RH) for 21 days (d). The weight loss (WL), respiration rate (RR), total soluble solids (TSS), pH, color (L*, a*, b*, chroma, and hue angle), proximate and mineral contents, phenolic and flavonoid contents, and other biochemical properties of the treated and untreated broccoli samples were evaluated throughout the storage duration. The lowest WL was observed when exposed to 0.5 mM of PUT. 1 mM CA and PUT treatments were affected by RR, depending on storage conditions. The lowest TSS content was observed in broccoli samples treated with 0.5 mM CA among all treatments. The chroma value of the samples was preserved by the 0.5 mM SA treatment. The most abundant element in broccoli samples was potassium in the control application, followed by a 1 mM SA treatment. In addition, the protein content was the highest in the 1 mM PUT treatment. The highest vitamin C was determined in the 1 mM CA treatment, and the most abundant vanillic acid was found in broccoli exposed to the 0.5 mM and treatment. Glucose content was determined at the lowest level in the 0.5 mM SA treatment, while higher increases occurred in other treatments. In terms of these findings, 1 mM CA, 1 mM SA, and 1 mM PUT delay WL, RR, and color degradation and prolong the storage life of broccoli samples stored at 4 ± 0.5°C. It was concluded that the biochemical content, fresh weight, and green color of broccoli samples throughout postharvest and storage can be maintained longer by exogenous application of these natural compounds. Therefore, we recommend 1 mM PUT and 1 mM CA treatments to maintain the quality of broccoli by minimizing losses in morphological properties, mineral, and biochemical compositions during postharvest storage.
Plant Functional Genomics Based on High‐Throughput CRISPR Library Knockout Screening: A Perspective
Authors: He Jianjie , Zeng Can , Li Maoteng 
Published: November 2023

AbstractPlant biology studies in the post‐genome era have been focused on annotating genome sequences’ functions. The established plant mutant collections have greatly accelerated functional genomics research in the past few decades. However, most plant genome sequences' roles and the underlying regulatory networks remain substantially unknown. Clustered, regularly interspaced short palindromic repeat (CRISPR)‐associated systems are robust, versatile tools for manipulating plant genomes with various targeted DNA perturbations, providing an excellent opportunity for high‐throughput interrogation of DNA elements’ roles. This study compares methods frequently used for plant functional genomics and then discusses different DNA multi‐targeted strategies to overcome gene redundancy using the CRISPR‐Cas9 system. Next, this work summarizes recent reports using CRISPR libraries for high‐throughput gene knockout and function discoveries in plants. Finally, this work envisions the future perspective of optimizing and leveraging CRISPR library screening in plant genomes' other uncharacterized DNA sequences.
Decreasing utilization for postoperative radiation therapy in locoregionally advanced medullary thyroid cancer
Authors: Maniakas Anastasios , Sullivan Andrew , Hu Mimi I. , Busaidy Naifa L. , Cabanillas Maria E. , Dadu Ramona , Waguespack Steven G. , Fisher Sarah B. , Graham Paul H. , Gross Neil D. , Grubbs Elizabeth G. , Perrier Nancy D. , Wang Jennifer R. , Gunn Brandon , Garden Adam S. , Megahed Romy , Navuluri Sriram , Li Xu , Williams Michelle D. , Zafereo Mark 
Published: November 2023

AbstractBackgroundUse of postoperative radiation therapy (PORT) in locoregionally advanced medullary thyroid cancer (MTC) remains controversial. The objective was to evaluate the effect of PORT on locoregional control (LRC) and overall survival (OS).MethodsRetrospective cohort study of 346 MTC patients separated into PORT and no‐PORT cohorts. Relative indications for PORT, as well as changes in patterns of treatment, were recorded.Results49/346 (14%) received PORT. PORT was associated with worse OS; adjusted HR = 2.0 (95%CI 1.3–3.3). PORT was not associated with improved LRC, even when adjusting for advanced stage (Stage III p = 0.892; Stage IV p = 0.101). PORT and targeted therapy were not associated with improved OS compared to targeted therapy alone; adjusted HR = 1.2 (95%CI 0.3–4.1).ConclusionsUse of PORT in MTC has decreased and its indications have become more selective, coinciding with the advent of effective targeted therapies. Overall, PORT was not associated with improved LRC or OS.
A hybrid entity structure for tax optimization of foreign direct investment in the U.S.
Authors: Kollruss Thomas 
Published: November 2023

AbstractThis article develops a new strategy for the (tax) optimization of foreign direct investments in the U.S. This strategy is particularly favorable for natural persons. By using a foreign upstream hybrid partnership, a substantial tax optimization of the current taxation of profits as well as the taxation of capital gains can be achieved. In addition, current and final losses may also be offset cross‐border to a certain extent in the case of an exemption under treaty law. This tax structuring idea is presented by way of example and explained on the basis of the country constellation U.S./Germany.
Development of photoluminescent viscose fibers integrated with polymer containing lanthanide‐doped phosphor
Authors: Hameed Yasmeen A. S. , Alamrani Nasser A. , Sallam Sahar , Ibarhiam Saham F. , Almahri Albandary , Alorabi Ali Q. , El‐Metwaly Nashwa M. 
Published: November 2023

AbstractSmart clothing refers to textiles that can sense an external stimulus by changing their physical properties such as colorimetric and fluorescent fabrics. The pad‐dry‐curing coloration approach was used to apply a luminous and hydrophobic composite coating onto cellulose‐based materials. This novel method includes incorporating phosphor nanoparticles made from lanthanide‐doped strontium aluminum oxide (LSAO) into room temperature vulcanizing silicone rubber (RTV). The LSAO nano‐sized particles (3–8 nm) must be mixed evenly throughout RTV without aggregation to allow for the formation of a colorless layer onto viscose surface. Pad‐dry‐curing the film onto viscose cloth worked well at room temperature. The contact angles of the luminous fibers enhanced from 138.6° to 158.2° as the LSAO ratio increased. The antimicrobial and ultraviolet (UV) protection of the LSAO‐finished viscose were investigated. The transparent fluorescent film on viscose surface was excited at 367 nm to display an emission peak at 518 nm. According to CIE Lab coordinates and luminescence analyses, the fluorescent viscose fibers showed various colors, including white under visible light, intense green beneath UV device, and greenish‐yellow under darkness. The comfort properties of the LSAO‐finished viscose were assessed by measuring their bend length and permeability to air. Transmission electron microscopic analysis of LSAO nanoparticles was explored. Energy dispersive x‐ray, x‐ray fluorescence, and scanning electron microscopy were utilized to describe the spectroscopic outcomes of the treated textiles. The colorfastness of the LSAO‐finished viscose fabrics was examined. The coated fabrics exhibited a non‐fatigable reversible luminous photochromism in response to UV illumination.Research Highlights
Multifunctional LSAO@RTV nanocomposite was pad‐dry‐cured onto viscose textile.
Photochromism to green under UV light and greenish‐yellow in the dark was detected.
Efficient antimicrobial, UV protective, and superhydrophobic activity were observed.
The antimicrobial properties were maintained for 24 washing cycles.
Pad‐dry‐cured viscose showed good comfortability and photostability.
Black‐Blood Magnetization Prepared 2 Rapid Acquisition Gradient Echoes: A Fast and Three‐Dimensional MR Black‐Blood T1 Mapping Technique for Quantitative Assessment of Atherosclerosis and Venous Thrombosis
Authors: Nie Yuhui , Lu Na , Liao Liping , Liu Zeping , Gu Anyan , Huang Xin , Tie Changjun , Liu Hongyan , Huang Zehe , Xie Guoxi 
Published: November 2023

BackgroundBlood flow signals may be a confounder in quantifying T1 values of plaque or thrombus and how to realize black‐blood T1 mapping remains a challenge task.PurposeTo develop a fast and three‐dimensional black‐blood T1 mapping technique for quantitative assessment of atherosclerosis and venous thrombosis.Study TypeSequence development and optimization via phantoms and volunteers as well as pilot prospective.Phantom and SubjectsNumerical simulations, a standard phantom, 8 healthy volunteers (mean age, 22 ± 1 years; 5 males), and 19 patients (mean age, 57 ± 14 years; 13 males) with atherosclerosis or venous thrombosis.Field Strength/Sequence3T/inversion recovery spin‐echo sequence (IR‐SE), magnetization prepared 2 rapid acquisition gradient echoes (MP2RAGE), and black‐blood prepared MP2RAGE (BB‐MP2RAGE).AssessmentThe black‐blood preparation (i.e., delay alternating with nutation for tailored excitation, DANTE) was incorporated into MP2RAGE for black‐blood T1 mapping. The BB‐MP2RAGE was optimized numerically based on the Bloch equation, and then the phantom study was performed to verify the accuracy of T1 mapping by BB‐MP2RAGE against IR‐SE and MP2RAGE. Preliminary clinical validation was prospectively performed to assess the flow suppression effect and its potential application in plaque and thrombosis identification.Statistical TestsPearson correlation test, Bland–Altman analysis, paired t‐test, and intraclass correlation coefficient. A P value <0.05 indicates a statistically significant difference.ResultsPhantom experiments showed comparable accuracy of T1 maps by BB‐MP2RAGE with IR‐SE and MP2RAGE (all r2 > 0.99); Compared to MP2RAGE, BB‐MP2RAGE effectively nulled the blood flow signals, and had a significant improvement in contrast‐to‐noise ratio between static tissue and blood (250.5 ± 66.6 vs. 91.9 ± 35.9). BB‐MP2RAGE can quantify plaque or thrombus T1 relaxation time with blood flow signal suppression.Data ConclusionAccurate T1 mapping with sufficient blood flow suppression was achieved by BB‐MP2RAGE. BB‐MP2RAGE has the potential to quantitatively characterize atherosclerosis and venous thrombosis.Level of Evidence1Technical EfficacyStage 1
Assessment of hepatitis B virus infection and interhost cellular responses using intrahepatic cholangiocyte organoids
Authors: Lim Chuan K. , Romeo Ornella , Tran Bang M. , Flanagan Dustin J. , Kirby Emily N. , McCartney Erin M. , Tse Edmund , Vincan Elizabeth , Beard Michael R. 
Published: November 2023

AbstractThe intrahepatic cholangiocyte organoids (ICOs) model was evaluated for host differences in hepatitis B virus (HBV) infection, cellular responses, antiviral and immunomodulator responses. Twelve ICOs generated from liver resections and biopsies were assessed for metabolic markers and functional HBV entry receptor expression throughout differentiation. Structural changes relevant to HBV infection were characterized using histology, confocal, and electron microscopy examinations. Optimal ICO culture conditions for HBV infection using HepAD38 (genotype D) and plasma‐derived HBV (genotype B and C) were described. HBV infection was confirmed using HBcAg immunostaining, qRT‐PCR (RNA, covalently closed circular DNA [cccDNA], extracellular DNA) and ELISA (HBsAg and HBeAg). Drug response to antiviral and immunosuppressive agent, and cellular responses (interferon‐stimulated genes [ISG]) to interferon‐α and viral mimic (PolyI:C) were assessed. ICOs underwent metabolic and structural remodeling following differentiation. Optimal HBV infection was achieved in well‐differentiated ICOs using spinoculation, with time and donor‐dependent increase in HBV RNA, cccDNA, extracellular DNA, HBeAg and HBsAg. Donor‐dependent drug responsiveness to entry inhibitor and JAK inhibitor was observed. Despite having a robust ISG response to interferon‐α and PolyI:C, HBV infection in ICOs did not upregulate ISGs. Human ICOs support HBV infection and replication with donor‐dependent variation in viral dynamics and cellular responses. These features can be utilized for the development of personalized drug testing platform for antivirals.
Unraveling the evolutionary patterns and phylogenomics of coronaviruses: A consensus network approach
Authors: Hu Geng‐Ming , Tai Yu‐Chen , Chen Chi‐Ming 
Published: November 2023

AbstractThe COVID‐19 pandemic emphasizes the significance of studying coronaviruses (CoVs). This study investigates the evolutionary patterns of 350 CoVs using four structural proteins (S, E, M, and N) and introduces a consensus methodology to construct a comprehensive phylogenomic network. Our clustering of CoVs into 4 genera is consistent with the current CoV classification. Additionally, we calculate network centrality measures to identify CoV strains with significant average weighted degree and betweenness centrality values, with a specific focus on RaTG13 in the beta genus and NGA/A116E7/2006 in the gamma genus. We compare the phylogenetics of CoVs using our distance‐based approach and the character‐based model with IQ‐TREE. Both methods yield largely consistent outcomes, indicating the reliability of our consensus approach. However, it is worth mentioning that our consensus method achieves an approximate 5000‐fold increase in speed compared to IQ‐TREE when analyzing the data set of 350 CoVs. This improved efficiency enhances the feasibility of conducting large‐scale phylogenomic studies on CoVs.
Understanding the resurgence of chikungunya virus during 2020−2021 in Pune, India, based on genomic analyses: A seven year study
Authors: Alagarasu Kalichamy , Patil Jayashri , Jadhav Santoshkumar , Chowdhury Deepika , Bote Minal , Punekar Madhura , More Ashwini , Kakade Mahadeo , Bachal Rupali , Gurav Yogesh , Cherian Sarah , Parashar Deepti 
Published: November 2023

AbstractA surge in chikungunya was observed during 2020‐21 in Pune district of Maharashtra, India. Whole genome sequencing and phylogenetic analysis of 21 samples/sequences revealed them as Indian ocean lineage of East Central South African genotype. Two distinct sequence clusters were found to circulate during 2020‐21; one with E1:K211E and E2:V264A mutations while the other had E1:I317V mutation along with E1:K211E and E2: V264A mutations. The former, the predominant cluster (n = 18), clustered with chikungunya virus (CHIKV) strains of pre 2014 period while the latter (n = 3) clustered with 2016−2018 period Indian strains. Though E1: A226V was not detected in any of the 21 sequences, several unique mutations were detected in the strains which might have played key roles in the enhanced virus transmission during the period. The study highlights parallel evolution, introduction from the neighboring regions and cocirculation of two sequence clusters of CHIKV in Pune. The complete genome data can be useful to determine how the circulating strains differ from candidate vaccines and might help to predict the protective efficacy of chikungunya vaccine candidates.
Serological analysis of severe acute respiratory syndrome coronavirus 2 infection in children with Kawasaki disease
Authors: Kondo Yotaro , Kawamura Yoshiki , Hattori Fumihiko , Nakai Hidetaka , Saito Kazuyoshi , Suzuki Daijiro , Kozawa Kei , Yoshikawa Tetsushi 
Published: November 2023

AbstractMultisystem inflammatory syndrome in children (MIS‐C) was reported as a severe complication of coronavirus disease 2019; an infection with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), and was suggested to be associated with Kawasaki disease (KD) in terms of severe systemic inflammation and mucocutaneous symptoms. Because severe gastrointestinal symptoms and systemic shock are more frequently observed with MIS‐C, patients with mild MIS‐C might have been diagnosed with KD. In this study, titers of IgG antibodies against the SARS‐CoV‐2 S (S‐IgG) and N proteins (N‐IgG) were measured in 99 serum samples collected from patients with KD treated between January 2020 and December 2021 to evaluate the relationship between KD and SARS‐CoV‐2 infection. S‐IgG were detected in only one patient out of 99 patients. This patient had coronavirus disease 2019 (COVID‐19) 10 months before KD onset, and was unlikely MIS‐C. According to characters of S‐IgG and N‐IgG, the patients was unlikely infected with SARS‐CoV‐2 just before the onset of KD. In addition to this study, the 26th Nationwide Survey and previous studies showed an association between KD and SARS‐CoV‐2 to be unlikely. In conclusion, SARS‐CoV‐2 infection was not observed in patients with KD until Delta predominance in Japan by the method of detecting SARS‐CoV‐2 IgG.
A multilevel model linking altruistic motivation to workplace safety: The role of servant leadership
Authors: He Yimin , Sheng Zitong , Griffin Mark , Yao Xiang 
Published: November 2023

SummaryPrevious research on the motivational factors of safety performance has predominantly focused on one's willingness to directly enact safety behaviors or safety‐specific motivation. The current study extends beyond this view and examines an additional motivational force, altruistic motivation, as a main predictor of employees' safety performance at both individual and team levels. Further, we provide that servant leadership serves as a critical precursor of employee altruistic motivation. A sample of 416 nurses in 42 workgroups and their respective supervisors from a hospital in China completed a two‐wave survey. Hierarchical linear modeling was used to analyze the data. The results indicated that altruistic motivation was positively related to safety performance at both individual and team levels, with a stronger effect at the team level, supporting a proportional theory of homology. Multilevel mediation results showed that servant leadership was positively related to altruistic motivation, which in turn positively led to safety performance at both individual and team levels. These findings highlight both the theoretical and practical importance of encouraging altruistic motivation to improve workplace safety.
In vitro bioaccessibility of biguanide‐related compounds and phenolics from yellow, red, and purple‐fleshed potatoes
Authors: Raigond Pinky , Parmar Vandana , Luthra Satish Kumar , Thakur Asha , Kumar Vinod , Dutt Som , Dalamu  , Jayanty Sastry S. , Singh Brajesh 
Published: November 2023

AbstractBackgroundDiabetes type II is growing rapidly as a result of the global shift in dietary and lifestyle habits. Although there are many pharmacological treatments for diabetes, incorporating foods with antidiabetic components into daily diet will be helpful. The present study compared the difference in the bioaccessibility of biguanide‐related compounds (BRCs, antidiabetic compounds) and phenolics from yellow, red, and purple potatoes.ResultsColor‐based averaged results showed that only biofortified red‐fleshed advanced potato clones showed higher bioaccessibility (14%) of BRCs compared to light yellow potatoes. Among all the tested compounds, only BRCs were released to maximum extent in the intestinal phase from all varieties and advance clones. This is the first report that revealed potatoes to be a good source of BRCs due to their high bioaccessibility. Bioaccessibility of phenolic compounds from boiled potatoes was 1.1‐fold higher in red colored, whereas almost 2‐fold higher in purple potatoes. Compared to light yellow potatoes, bioaccesibility of 3‐caffeoylquinic acid, 5‐O‐caffeoylquinic acid and 4‐O‐caffeoylquinic acid was 5.1, 5.7, 4.6 fold higher in boiled red potatoes, respectively, and 4.6, 3.7, and 6.9 fold higher in boiled purple potatoes, respectively. Total anthocyanins showed color‐dependent bioaccessibility and were 3.2 and 16.2 fold more bioaccessible from red and purple boiled potatoes, respectively, compared to light yellow potatoes.ConclusionDue to high bioaccessibility of phenolics, anthocyanins, and BRCs, purple and red potatoes can play pivotal role in health improvement of consumers worldwide. However, further detailed bioavailability studies are required to confirm these results.