bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análisis de los retos logísticos de las exportaciones ecuatorianas hacia Colombia en el periodo 2009 al 2019
Authors: Mantilla Centeno Farid Alfredo , Altamirano Flores Jorge Enrique , Vásquez Bernal Jorge Vicente 
Published: October 2023

El presente artículo tuvo como objetivo analizar los retos logísticos que afrontaron las exportaciones ecuatorianas hacia Colombia enel periodo del 2009- 2019. Se aplicó el método analítico bajo un diseño cuali-cuantitavo para el análisis de datos e información, expresando los resultados en un diagnóstico PESTEL enfocado en el manejo logístico, flujo de mercancía, transportes y vías de acceso, requisitos, normativas, aranceles y planes de exportación del Servicio Nacional de Aduanas del Ecuador. Con lo anterior, se determinó el entorno de los factores que incidieron en el servicio logístico de las exportaciones de Ecuador hacia Colombia, permitiendo consolidar el potencial logístico del país que está en ascenso y que necesita soporte del sector público para mitigar y superar los desafíos presentados en exportación de mercancías hacia Colombia, así como también la deficiente infraestructura aduanera quese ve limitada por la escasa inversión pública en la última década.
Palabras clave: Colombia, Ecuador, exportaciones, importaciones, logística
AbstractThe objective of this article was to analyze the logistical challenges faced by Ecuadorian exports to Colombia in the period 2009 - 2019.The analytical method was applied under a qualitative-quantitative design for the analysis of data and information, expressing the results in a diagnosis. PESTEL, focused on logistics management, merchandise flow, transport and access roads, requirements, regulations, tariffs and export plans of the National Customs Service of Ecuador. With the above, the environment of the factors that affected the logistics service of exports from Ecuador to Colombia was determined, allowing the consolidation of the logistics potential of the country that is on the rise and that needs support from the public sector to mitigate and overcome the challenges presented in export of merchandise to Colombia, as well as the deficient customs infrastructure that is limited by the low public investment in thelast decade.
Keywords: Colombia, Ecuador, exports, imports, logistics
Effect of composite conjugated materials on tissue healing during exercise rehabilitation training
Authors: Li Jie , Zhang Jing 
Published: October 2023

The application of traditional materials to tissue healing in sports rehabilitation training has problems such as poor effect, high rejection reaction, and slow healing speed. It also brings many challenges to the development of sports rehabilitation training. This article aims to explore the impact of composite conjugated materials on tissue healing to promote rapid and efficient tissue healing and improve the effect of sports rehabilitation training. Through research and analysis, this article found that composite conjugated materials have unique biocompatibility and can promote cell growth and differentiation. In skin tissue healing, composite conjugated materials can control the release rate and duration of drugs to promote skin healing. During the fracture healing process, conjugated materials can provide growth factors and extracellular matrix components, stimulate bone cell proliferation and differentiation, and promote fracture healing. In terms of soft tissue injuries, composite conjugated materials serve as supporting structures or matrices, providing a favorable environment for the regeneration of damaged tissue. In the regulation of inflammatory responses, composite conjugated materials reduce inflammatory responses and accelerate the healing process by modulating immune responses. The results of this study show that 1 week after the experiment, the skin healing rates of the control group and the experimental group were 42.55% and 58.17% respectively; 5 weeks after the experiment, the skin healing rates of the control group and the experimental group were 51.28% and 73.24% respectively. After 1, 2, 3, 4, and 5 weeks of experiment, it was found that the average tissue repair rates of the control group were 44.03%, 54.18%, 58.40%, 67.08%, and 72.09% respectively, and the average tissue repair rates of the experimental group were 52.18%, 61.91%, 63.40%, 74.61%, and 85.05% respectively. This study highlights the huge potential of composite conjugated materials in promoting tissue healing and tissue repair, and is of great significance for promoting technological progress in the field of sports rehabilitation and improving rehabilitation effects.
Significance of RNA N6-methyladenosine regulators in the diagnosis and subtype classification of coronary heart disease using the Gene Expression Omnibus database
Authors: Jiang Yu , Pan Yaqiang , Long Tao , Qi Junqing , Liu Jianchao , Zhang Mengya 
Published: October 2023

BackgroundMany investigations have revealed that alterations in m6A modification levels may be linked to coronary heart disease (CHD). However, the specific link between m6A alteration and CHD warrants further investigation.MethodsGene expression profiles from the Gene Expression Omnibus (GEO) databases. We began by constructing a Random Forest model followed by a Nomogram model, both aimed at enhancing our predictive capabilities on specific m6A markers. We then shifted our focus to identify distinct molecular subtypes based on the key m6A regulators and to discern differentially expressed genes between the unique m6A clusters. Following this molecular exploration, we embarked on an in-depth analysis of the biological characteristics associated with each m6A cluster, revealing profound differences between them. Finally, we delved into the identification and correlation analysis of immune cell infiltration across these clusters, emphasizing the potential interplay between m6A modification and the immune system.ResultsIn this research, 37 important m6Aregulators were identified by comparing non-CHD and CHD patients from the GSE20680, GSE20681, and GSE71226 datasets. To predict the risk of CHD, seven candidate m6A regulators (CBLL1, HNRNPC, YTHDC2, YTHDF1, YTHDF2, YTHDF3, ZC3H13) were screened using the logistic regression model. Based on the seven possible m6A regulators, a nomogram model was constructed. An examination of decision curves revealed that CHD patients could benefit from the nomogram model. On the basis of the selected relevant m6A regulators, patients with CHD were separated into two m6A clusters (cluster1 and cluster2) using the consensus clustering approach. The Single Sample Gene Set Enrichment Analysis (ssGSEA) and CIBERSORT methods were used to estimate the immunological characteristics of two separate m6A Gene Clusters; the results indicated a close association between seven candidate genes and immune cell composition. The drug sensitivity of seven candidate regulators was predicted, and these seven regulators appeared in numerous diseases as pharmacological targets while displaying strong drug sensitivity.Conclusionm6A regulators play crucial roles in the development of CHD. Our research of m6A clusters may facilitate the development of novel molecular therapies and inform future immunotherapeutic methods for CHD.
Prevalence and temporal trends of prostate diseases among inpatients with cardiovascular disease: a nationwide real-world database survey in Japan
Authors: Kaneta Kohei , Tanaka Atsushi , Nakai Michikazu , Sumita Yoko , Kaneko Hidehiro , Noguchi Mitsuru , Node Koichi 
Published: October 2023

IntroductionBenign prostate hyperplasia (BPH) and prostate cancer (PCa) are major prostate diseases that potentially share cardiometabolic risk factors and an elevated risk for cardiovascular disease (CVD). However, the prevalence of prostate diseases among patients with established CVD remains unclear.Materials and methodsThis nationwide retrospective study assessed the prevalence and temporal trend of prostate diseases (i.e., BPH or PCa) among patients hospitalized for CVDs in Japan. We used a claims database (the Japanese Registry of All Cardiac and Vascular Diseases–Diagnosis Procedure Combination), which included data on 6,078,487 male patients recorded from 1,058 hospitals between April 2012 and March 2020. We conducted the Cochran–Armitage trend test and calculated the adjusted odds ratio (aOR) with 95% confidence intervals (CIs).ResultsThe prevalence of prostate diseases over the entire study period was 5.7% (BPH, 4.4%; PCa, 1.6%). When dividing the overall cohort into age categories (&lt;65, 65–74, and ≥75 years old), the prevalence was 1.1%, 4.7%, and 9.9%, respectively (P for trend &lt;0.05). In addition, the annual prevalence showed a modest increasing trend over time. Patients admitted for heart failure (HF) were significantly associated with a higher incidence of coexisting prostate diseases than those admitted for non-HF causes [aOR 1.02 (95% CI, 1.01–1.03)] or acute coronary syndrome [aOR 1.19 (95% CI, 1.17–1.22)].ConclusionsThe nationwide real-world database revealed that the prevalence of prostate diseases is increasing among patients hospitalized for CVD, particularly HF. Attention to detailed causality and continued surveillance are needed to further clarify the clinical characteristics of prostate diseases among patients with CVD.
On the external forcing of global eruptive activity in the past 300 years
Authors: Le Mouël J-L. , Gibert D. , Courtillot V. , Dumont S. , de Bremond d'Ars J. , Petrosino S. , Zuddas P. , Lopes F. , Boulé J-B. , Neves M. C. , Custódio S. , Silveira G. , Kossobokov V. , Coen L. , Gèze M. 
Published: October 2023

The decryption of the temporal sequence of volcanic eruptions is a key step in better anticipating future events. Volcanic activity results from a complex interaction between internal and external processes, with time scales spanning multiple orders of magnitude. We review periodicities that have been detected or correlated with volcanic eruptions/phenomena and interpreted as resulting from external forces. Taking a global perspective and longer time scales than a few years, we approach this interaction by analyzing three time-series using singular spectral analysis: the global number of volcanic eruptions (NVE) between 1700 and 2022, the number of sunspots (ISSN), a proxy for solar activity, and polar motion (PM) and length of day (lod), two proxies for gravitational force. Several pseudo-periodicities are common to NVE and ISSN, in addition to the 11-year Schwabe cycle that has been reported in previous work, but NVE shares even more periodicities with PM. These quasi-periodic components range from −5 to −130 years. We interpret our analytical results in light of the Laplace’s paradigm and propose that, similarly to the movement of Earth’s rotation axis, global eruptive activity is modulated by commensurable orbital moments of the Jovian planets together with Pluto, whose influence is also detected in solar activity.
Molecular interplay between ecdysone receptor and retinoid X receptor in regulating the molting of the Chinese mitten crab, Eriocheir sinensis
Authors: Chen Xiaowen , Hou Xin , Yang Hao , Liu Hao , Wang Jun , Wang Chenghui 
Published: October 2023

PurposeMolting is a pivotal biological process regulated by the ecdysteroid signaling pathway that requires molecular coordination of two transcription factors, Ecdysone receptor (EcR) and ultraspiracle (USP) in arthropods. However, the molecular interplay of EcR and Retinoid X receptor (RXR), the crustacean homolog of USP in the ecdysteroid signaling pathway, is not well understood.MethodsIn this study, we conducted temporal and spatial expression, co-immunoprecipitation (CO-IP), and luciferase reporter assay experiments to investigate the molecular function and interplay of EcR and RXR during the molting process of the Chinese mitten crab, Eriocheir sinensis.ResultsThe results showed that the expression level of RXR was more stable and significantly higher than EcR during the entire molting process. However, the expression level of EcR fluctuated dynamically and increased sharply at the premolt stage. The CO-IP and luciferase reporter assay results confirmed the molecular interplay of EcR and RXR. The heterodimer complex formed by the two transcription factors significantly induced the transcription of E75, an essential gene in the ecdysteroid signaling pathway.ConclusionsOur study unveiled the diverse molecular function and molecular interplay of EcR and RXR; RXR is possibly a “constitutive-type” gene, and EcR is possibly a vital speed-limiting gene while both EcR and RXR are required to initiate the ecdysteroid signaling cascade, which may be indispensable for molting regulation in E. sinensis. The results provide a theoretical basis for the endocrine control of molting in E. sinensis and novel insights into the molecular mechanism of molting mediated by the ecdysteroid signaling pathway in crustaceans.
IGFBP2 from a novel copper metabolism-associated biomarker promoted glioma progression and response to immunotherapy
Authors: Luo Qisheng , Zhuang Junhong , Zheng Dandan , Miao Changfeng , Luo Hongcheng , Peng Jun , Zheng Chuanhua , Qin Chengjian , Lan Chuanliu , Chen Meiqin , Xia Ying , Huang Deyou , Chen Zigui 
Published: October 2023

IntroductionCopper metabolism encompasses all cellular metabolic processes involving copper ions and plays a significant role in the pathogenesis of diseases, including cancer. Furthermore, copper is intricately involved in various processes related to nucleotide metabolism. However, a comprehensive analysis of copper metabolism in gliomas remains lacking despite its importance.MethodsTo address this gap, glioma patients were stratified based on the expression levels of copper metabolism-related genes. By utilizing machine learning techniques, a novel copper metabolism-associated biomarker was developed. The potential of this biomarker in prognosis, mutation analysis, and predicting immunotherapy response efficiency in gliomas was systematically investigated.ResultsNotably, IGFBP2, identified as a glioma tumor promoter, was found to promote disease progression and influence immunotherapy response. Additionally, glioma-derived IGFBP2 was observed to enhance microglial migration. High IGFBP2 expression in GBM cells facilitated macrophage interactions through the EGFR, CD63, ITGB1, and CD44 signaling pathways. Discussion: Overall, the copper metabolism-associated biomarker shows promising potential to enhance the clinical management of gliomas, offering valuable insights into disease prognosis and treatment strategies.
Characterization of the bacterial microbiome of non-hematophagous bats and associated ectoparasites from Brazil
Authors: André Marcos Rogério , Ikeda Priscila , Lee Daniel Antônio Braga , do Amaral Renan Bressianini , Carvalho Lucas Amoroso Lopes , Pinheiro Daniel Guariz , Torres Jaire Marinho , de Mello Victória Valente Califre , Rice Gregory K. , Cer Regina Z. , Lourenço Elizabete Captivo , Oliveira Carisa Elisei , Herrera Heitor Miraglia , Barros-Battesti Darci Moraes , Machado Rosangela Zacarias , Bishop-Lilly Kimberly A. , Dalgard Clifton L. , Dumler J. Stephen 
Published: October 2023

IntroductionBats, along with their ectoparasites, harbor a wide diversity of symbiotic and potential pathogenic bacteria. Despite the enormous diversity of bats (181 species), few studies aimed to investigate the bacterial microbiome of Brazilian chiropterans and associated ectoparasites. This study aimed to characterize the bacterial microbiome of non-hematophagous bats and associated Streblidae flies and Macronyssidae and Spinturnicidae mites in the state of Mato Grosso do Sul, midwestern Brazil.MethodsOral and rectal swabs were collected from 30 bats (Artibeus lituratus [n = 13], Artibeus planirostris [n  =  9], Eptesicus furinalis [n = 5], Carollia perspicillata [n = 2], and Platyrrhinus lineatus [n = 1]). In addition, a total of 58 mites (15 Macronyssidae and 43 Spinturnicidae) and 48 Streblidae bat flies were collected from the captured bats. After DNA extraction and purification, each sample’s bacterial composition was analyzed with metagenomic sequencing.ResultsThe microbiome composition of both oral and rectal bat swab samples showed that Gammaproteobacteria was the most abundant bacterial class. Spiroplasma, Wolbachia and Bartonella represented the most abundant genera in Streblidae flies. While Wolbachia (Alphaproteobacteria) was the most abundant genus found in Spinturnicidae, Arsenophonus (Gammaproteobacteria) was found in high abundance in Macronyssidae mites. In addition to characterizing the microbiome of each sample at the class and genus taxonomic levels, we identified medically significant bacteria able to infect both animals and humans in oral (Streptococcus and Anaplasma) and rectal swabs (Enterobacter, Klebsiella, Escherichia, Enterococcus, Streptococcus), Macronyssidae (Anaplasma, Bartonella, Ehrlichia) and Spinturnicidae (Anaplasma, Bartonella) mites as well as Streblidae flies (Spiroplasma, Bartonella).Discussion and conclusionBesides expanding the knowledge on the bacterial microbiome of non-hematophagous bats and Streblidae flies from Brazil, the present work showed, for the first time, the bacterial community of bat-associated Macronyssidae and Spinturnicidae mites.
Nomogram model of functional outcome for endovascular treatment in patients with acute basilar artery occlusion
Authors: Li Lei , Lv Jin , Han Jian-jia , Gao Yuan , Yan Zhao-xuan , Wu Qi , Zhang Xiao-li , Gao Feng 
Published: October 2023

Background and purposeThe efficacy and safety of endovascular treatment (EVT) in acute basilar artery occlusion (ABAO) has been confirmed by four randomized clinical trials. Nevertheless, the predictors of a 90-day favorable outcome after EVT have not been elucidated. We attempted to establish a nomogram for the prediction of a 90-day favorable outcome in ABAO patients with EVT.MethodsClinical data of ABAO patients with EVT were obtained from two nationwide clinical trial registries in China. Factors associated with a 90-day favorable outcome were screened by multivariable step-wise regression on the basis of univariable analysis. A nomogram was established to predict 90-day favorable outcome after EVT.ResultsThe proportion of ABAO patients with a favorable outcome was 41.53% (157/378). Seven variables, including baseline National Institutes of Health Stroke Scale (NIHSS) &lt;20 [odds ratio (OR): 8.330; P-value &lt; 0.0001], posterior circulation Alberta Stroke Program Early CT (pc-ASPECT) score ≥7 (OR: 1.948; P-value = 0.0296), Pons-Midbrain Index (PMI) score &lt; 2 (OR: 2.108; P-value = 0.0128), Posterior Circulation Collateral Score (PC-CS) ≥5 (OR: 3.288; P-value &lt; 0.0001), local anesthesia (OR: 0.389; P-value = 0.0017), time from onset to recanalization (OTR) &lt;330 min (OR: 2.594; P-value = 0.0013), and no occurrence of early neurological deterioration (END; OR: 0.039; P-value &lt; 0.0001) were included into the nomogram, with C-index values of 0.8730 and 0.8857 in the training and the internal validation set, respectively.ConclusionsThe proposed nomogram provided a reliable prognostic scale, which can be employed in clinical settings for the selection and clinical management of ABAO patients.Registrationhttps://www.clinicaltrials.gov, identifier: NCT03370939.
The external evocation and movement-related modulation of motor cortex inhibition in children and adolescents with Tourette syndrome – a TMS/EEG study
Authors: Schmidgen Julia , Konrad Kerstin , Roessner Veit , Bender Stephan 
Published: October 2023

ObjectiveThis study tested the reactivity of motor cortex inhibition to different intensities of external stimulation by transcranial magnetic stimulation (TMS) and its internal modulation during different motor states in children and adolescents with Tourette syndrome.MethodsTMS-evoked N100 served as an indirect measure of GABAB receptor function which is related to cortical inhibition. Combined TMS/EEG was used to analyze the TMS-evoked N100 component evoked by different stimulation intensities as well as during resting condition, movement preparation (contingent negative variation task) and movement execution. The study included 18 early adolescents with Tourette syndrome and 15 typically developing control subjects.ResultsTMS-evoked N100 showed a less steep increase with increasing TMS intensity in Tourette syndrome together with less modulation (disinhibition) over the primary motor cortex during the motor states movement preparation and movement execution. Children with Tourette syndrome showed equally high N100 amplitudes at 110% resting motor threshold (RMT) intensity during resting condition and a parallel decline of RMT and N100 amplitude with increasing age as control subjects.ConclusionOur study yields preliminary evidence that modulation of motor cortical inhibitory circuits, during external direct stimulation by different TMS intensities and during volitional movement preparation and execution is different in children and adolescents with Tourette syndrome compared to controls. These results suggest that a reduced resting motor cortical inhibitory “reserve” could contribute to the production of unwanted movements. Our findings are compatible with increased regulation of motor cortex excitability by perception-action binding in Tourette syndrome instead of top-down / motor regulation and need to be replicated in further studies.
A novel approach to assess diet diversity: a development of the Nutritional Functional Diversity indicator
Authors: Di Maso Matteo , Bravi Francesca , Polesel Jerry , Dal Maso Luigino , Patel Linia , La Vecchia Carlo , Ferraroni Monica 
Published: October 2023

Background and aimsDiversity is a key element of diet quality. The Food Variety Score (FVS) is used to assess diversity, especially in low- and middle-income countries. It sums up the number of foods consumed ignoring their nutrient content. A more suitable index should combine the number of foods consumed and their nutritional composition. We adapted the Nutritional Functional Diversity indicator (NFD), proposed by ecologists, to measure diversity in the human diet. We compared NFD and FVS evaluating subjects’ distributions across quartiles of the two diversity indices. To evaluate which one reflected a higher diet quality, we estimated associations between these two diversity indices and diet quality measures, i.e., the Mediterranean Diet Score (MDS) and the Healthy Eating Index-2015 (HEI-2015). Associations were expressed by odds ratios (OR) and corresponding 95% confidence intervals (CI).Materials and methodsWe used the data of controls only derived from an integrated series of hospital-based case-control cancer studies conducted in different Italian areas. The NFD identifies groups of foods based on a set of nutrients according to a cluster analysis. Some steps are required: creating a food-nutrient matrix; clustering of the Euclidean food-food distance matrix to identify groups of foods with nutritional (dis)similarities; and calculating the NFD as the ratio between the sum of branch lengths of the dendrogram belonging to the number of foods consumed by individuals (i.e., subject-specific diversity) and the sum of all branch lengths of the dendrogram (i.e., maximal diversity).ResultsMore than one quarter of individuals (28.4%) were differently classified within quartiles of the two diversity indices. For both indices, increasing the diversity level increased the risk for adhering to MDS (OR for NFD = 11.26; 95% CI: 7.88–16.09, and OR for FVS = 6.80; 95% CI: 4.84–9.54) and to HEI-2015 (OR for NDF = 2.86; 95% CI: 2.39–3.42, and OR for FVS = 2.72; 95% CI: 2.27–3.26). Associations were stronger for NFD.ConclusionOur findings showed a greater ability of NFD to assess diet quality quantifying the degree of diversity.