bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Gene Signature of Nociceptors Innervating Mouse Molar Teeth, Cranial Meninges, and Cornea
Authors: Sotelo-Hitschfeld Pamela , Bernal Laura , Nazeri Masoud , Renthal William , Brauchi Sebastian , Roza Carolina , Zimmermann Katharina 
Published: January 2024

BACKGROUND:
The trigeminal ganglion (TG) collects afferent sensory information from various tissues. Recent large-scale RNA sequencing of neurons of the TG and dorsal root ganglion has revealed a variety of functionally distinct neuronal subpopulations, but organ-specific information is lacking.

METHODS:
To link transcriptomic and tissue-specific information, we labeled small-diameter neurons of 3 specific subpopulations of the TG by local application of lipophilic carbocyanine dyes to their innervation site in the dental pulp, cornea, and meninges (dura mater). We then collected mRNA-sequencing data from fluorescent neurons. Differentially expressed genes (DEGs) were analyzed and subjected to downstream gene set enrichment analysis (GSEA), and ion channel profiling was performed.

RESULTS:
A total of 10,903 genes were mapped to the mouse genome (>500 reads). DEG analysis revealed 18 and 81 genes with differential expression (log2 fold change > 2, P
adj < .05) in primary afferent neurons innervating the dental pulp (dental primary afferent neurons [DPAN]) compared to those innervating the meninges (meningeal primary afferent neurons [MPAN]) and the cornea (corneal primary afferent neurons [CPAN]). We found 250 and 292 genes differentially expressed in MPAN as compared to DPAN and to CPAN, and 21 and 12 in CPAN as compared to DPAN and MPAN. Scn2b had the highest log2 fold change when comparing DPAN versus MPAN and Mmp12 was the most prominent DEG when comparing DPAN versus CPAN and, CPAN versus MPAN. GSEA revealed genes of the immune and mitochondrial oxidative phosphorylation system for the DPAN versus MPAN comparison, cilium- and ribosome-related genes for the CPAN versus DPAN comparison, and respirasome, immune cell- and ribosome-related gene sets for the CPAN versus MPAN comparison. DEG analysis for ion channels revealed no significant differences between the neurons set except for the sodium voltage-gated channel beta subunit 2, Scn2b. However, in each tissue a few ion channels turned up with robust number of reads. In DPAN, these were Cacna1b, Trpv2, Cnga4, Hcn1, and Hcn3, in CPAN Trpa1, Trpv1, Cacna1a, and Kcnk13 and in MPAN Trpv2 and Scn11a.

CONCLUSIONS:
Our study uncovers previously unknown differences in gene expression between sensory neuron subpopulations from the dental pulp, cornea, and dura mater and provides the basis for functional studies, including the investigation of ion channel function and their suitability as targets for tissue-specific analgesia.
Metabolic and Perceptual Responses to Constant Heart Rate Exercise at Vigorous Intensities in Women
Authors: GUSTAVE DJADMANN , MITCHINSON CLARA J. , SUCCI PASQUALE J. , BENITEZ BRIAN , KWAK MINYOUNG , LANPHERE KATHRYN R. , CLASEY JODY L. , BERGSTROM HALEY C. 
Published: January 2024

ABSTRACT

Purpose
This study quantified the metabolic demands (oxygen uptake (V̇O2)), power output adjustments, changes in the V̇O2/power output ratio, and perceptual responses (rating of perceived exertion (RPE)) during constant heart rate (HR) exercise performed within the vigorous intensity range (77%–95% HRpeak).

Methods
Twelve women (mean ± SD age, 22 ± 4 yr) performed a graded exercise test to exhaustion to determine peak parameters, and three randomly ordered, constant HR trials to exhaustion or for 60 min at the lower (HRL = 77% HRpeak), middle (HRM = 86% HRpeak), and higher (HRH = 95% HRpeak) end of the vigorous intensity range. Time course of changes and patterns of responses were examined for V̇O2, power output, V̇O2/power output, and RPE for the composite and for each subject.

Results
Across the HRL (time to exhaustion (T
lim) = 56.3 ± 9.9 min), HRM (51.8 ± 13.5 min), and HRH (27.2 ± 17.7 min) trials, V̇O2 and power output decreased quadratically (P < 0.05) relative to the initial value from 10% to 100% of T
lim, whereas the V̇O2/power output increased quadratically from 20% to 100% T
lim, and RPE increased linearly from 50% to 100% T
lim. The V̇O2 and RPE, collapsed across time, for HRL (54.3% ± 3.3% V̇O2peak, 11 ± 1.5 RPE) were lower than HRM (64.9% ± 4.5% V̇O2peak, 14 ± 1.7 RPE), and both were lower than HRH (80.1% ± 4.1% V̇O2peak, 17 ± 1.4 RPE). None of the 12 subjects at HRL, 6 at HRM, and 7 at HRH were within the vigorous V̇O2 range.

Conclusions
The HRL was not sufficient to meet the desired metabolic intensity for vigorous exercise, whereas the middle to higher end of the range elicited a V̇O2 within the prescribed range of only ~50%–60% of the subjects. This study indicated that exercise held constant at a percentage of HRpeak cannot consistently be used to prescribe a desired metabolic stimulus.
Philibert de Naillac, Master of Rhodes: 1396‒1421
Authors: Luttrell Anthony 
Published: January 2024

Philibert de Naillac belonged to the nobility of Berri. He entered the military-religious order of the Hospital and by 1374 held the Commandery of Lureil in the Priory of Auvergne; in 1390 he became Prior of Aquitaine and was due to travel to Rhodes. Apparently in 1395 he again left for Rhodes where, probably on 6 May 1396, he was elected Master. Soon after he led a Hospitaller contingent into the Black Sea and up the Danube to Nikopolis to join a crusade which on 25 September 1396 was defeated by the Turks; Naillac escaped, rescuing Sigismund of Hungary from captivity or death. Subsequently the Hospitallers defended Constantinople and the Morea against the Turks, built the large “Naillac” tower in Rhodes harbour, and in 1406/1407 constructed a castle at Bodrum on the Anatolian mainland opposite Kos. Meanwhile the Hospital’s holy warfare at sea included the semi-piratical corso. Naillac’s administrative tasks and visitations were extensive in both East and West. The Hospital's manpower and money reaching Rhodes were seriously reduced by defections provoked by the papal schism, and in 1409 the Master travelled to Pisa where a council elected a new pope with Naillac guarding the conclave. He continued to work for a settlement and in 1417 he again guarded the conclave at Constance where the schism was largely ended. The schism within the Hospital itself was also slowly eliminated. In 1420 Naillac returned to Rhodes where he held a chapter general which passed important legislation. He died in 1421 after a lengthy rule which, not without some dubious aspects, did much to preserve the Hospitaller order.
Restoration of Discoloured Incisors with Ceramic Veneers: A Workflow Case Report
Authors: Albino Luis Gustavo Barrote , Chaves Eduardo Trota , Pereira de Lima Verônica , Lima Giana da Silveira 
Published: January 2024

This case reports the treatment of two discoloured incisors with feldspar ceramic veneers using an integrated work approach, and the active participation of a dental technician. The patient presented an upper left central incisor with a composite restoration, and a lateral incisor with discolouration after trauma and endodontic treatment. Planning was developed in collaboration with the technician, the decision was to perform ceramic veneers on the discoloured teeth. Central incisor previous restoration was included in preparation, and lateral preparation was limited to create enough space for the veneer. This case highlights the importance of communication between patient, clinician, and technician to achieve excellent results in restorative dentistry. CPD/Clinical Relevance: Feldspar ceramic veneers might be a good option for masking light and mediumly discoloured substrates.
Bleaching Complications: ‘Greening’ in a Post-crowned Tooth
Authors: Feng Qingmei Joy , Kelleher Martin 
Published: January 2024

This article describes the management of a patient who experienced an unexpected and unattractive ‘greening’ of an anterior tooth following a course of bleaching. It examines the reasons for this phenomenon and describes a method for disguising the discolouration in a minimally invasive manner that can be carried out in general practice. CPD/Clinical Relevance: It is important that multiple post-operative complications are managed in a minimally invasive way whenever possible.
Genetic investigation of the ubiquitin-protein ligase E3A gene as putative target in Angelman syndrome
Authors: Manoubi Wiem , Mahdouani Marwa , Hmida Dorra , Kdissa Ameni , Rouissi Aida , Turki Ilhem , Gueddiche Neji , Soyah Najla , Saad Ali , Bouwkamp Christian , Elgersma Ype , Mougou-Zerelli Soumaya , Gribaa Moez 
Published: January 2024

BACKGROUND
Angelman syndrome (AS) is caused by maternal chromosomal deletions, imprinting defects, paternal uniparental disomy involving chromosome 15 and the ubiquitin-protein ligase UBE3A gene mutations. However the genetic basis remains unclear for several patients.
AIM
To investigate the involvement of UBE3A gene in AS and identifying new potential genes using exome sequencing.
METHODS
We established a cohort study in 50 patients referred to Farhat Hached University Hospital between 2006 and 2021, with a strong suspicion of AS and absence of chromosomal aberrations. The UBE3A gene was screened for mutation detection. Two unrelated patients issued from consanguineous families were subjected to exome analysis.
RESULTS
We describe seven UBE3A variants among them 3 none previously described including intronic variants c.2220+14T>C (intron14), c.2507+43T>A (Exon15) and insertion in Exon7: c.30-47_30-46. The exome sequencing revealed 22 potential genes that could be involved in AS-like syndromes that should be investigated further.
CONCLUSION
Screening for UBE3A mutations in AS patients has been proven to be useful to confirm the diagnosis. Our exome findings could rise to new potential alternative target genes for genetic counseling.
Inetetamab combined with pyrotinib and chemotherapy in the treatment of breast cancer brain metastasis: A case report
Authors: Dou Qing-Qing , Sun Ting-Ting , Wang Guo-Qiang , Tong Wei-Bing 
Published: January 2024

BACKGROUND
Breast cancer brain metastasis (BCBM) is an advanced breast disease that is difficult to treat and is associated with a high risk of death. Patient prognosis is usually poor, with reduced quality of life. In this context, we report the case of a patient with HER-2-positive BCBM treated with a macromolecular mAb (inetetamab) combined with a small molecule tyrosine kinase inhibitor (TKI).
CASE SUMMARY
The patient was a 58-year-old woman with a 12-year history of type 2 diabetes. She was compliant with regular insulin treatment and had good blood glucose control. The patient was diagnosed with invasive carcinoma of the right breast (T3N1M0 stage IIIa, HER2-positive type) through aspiration biopsy of the ipsilateral breast due to the discovery of a breast tumor in February 2019. Immunohistochemistry showed ER (-), PR (-), HER-2 (3+), and Ki-67 (55-60%+). Preoperative neoadjuvant chemotherapy, i.e. , the AC-TH regimen (epirubicin, cyclophosphamide, docetaxel-paclitaxel, and trastuzumab), was administered for 8 cycles. She underwent modified radical mastectomy of the right breast in November 2019 and received tocilizumab targeted therapy for 1 year. Brain metastasis was found 9 mo after surgery. She underwent brain metastasectomy in August 2020. Immunohistochemistry showed ER (-) and PR. (-), HER-2 (3+), and Ki-67 (10-20%+). In November 2020, the patient experienced headache symptoms. After an examination, tumor recurrence in the original surgical region of the brain was observed, and the patient was treated with inetetamab, pyrotinib, and capecitabine. Whole-brain radiotherapy was recommended. The patient and her family refused radiotherapy for personal reasons. In September 2021, a routine examination revealed that the brain tumor was considerably larger. The original systemic treatment was continued and combined with intensity-modulated radiation therapy for brain metastases, followed by regular hospitalization and routine examinations. The patient’s condition is generally stable, and she has a relatively high quality of life. This case report demonstrates that in patients with BCBM and resistance to trastuzumab, inetetamab combined with pyrotinib and chemotherapy can prolong survival.
CONCLUSION
Inetetamab combined with small molecule TKI drugs, chemotherapy and radiation may be an effective regimen for maintaining stable disease in patients with BCBM.
Carrimycin in the treatment of acute promyelocytic leukemia combined with pulmonary tuberculosis: A case report
Authors: Yang Fu-Yu , Shao Lei , Su Jie , Zhang Zhen-Meng 
Published: January 2024

BACKGROUND
Pulmonary tuberculosis (PTB) is prevalent in immunocompromised populations, including patients with hematologic malignancies, human immunodeficiency virus infections, and chronic diseases. Effective treatment for acute promyelocytic leukemia (APL) combined with PTB is lacking. These patients show an extremely poor prognosis. Therefore, studies should establish efficient treatment options to improve patient survival and prognosis.
CASE SUMMARY
A 60-year-old male with pain in the right side of his chest and a fever for 4 d visited the outpatient department of our hospital. Peripheral blood smear revealed 54% blasts. Following bone marrow examinations, variant APL with TNRC18-RARA fusion gene was diagnosed. Chest computed tomography scan showed bilateral pneumonitis with bilateral pleural effusions, partial atelectasis in the lower lobes of both lungs, and the bronchoalveolar lavage fluid gene X-Pert test was positive, indicative of PTB. Carrimycin, ethambutol (EMB), and isoniazid (INH) were administered since he could not receive chemotherapy as the WBC count decreased continuously. After one week of treatment with carrimycin, the patient recovered from fever and received chemotherapy. Chemotherapy was very effective and his white blood cells counts got back to normal. After being given five months with rifampin, EMB and INH and chemotherapy, the patient showed complete remission from pneumonia and APL.
CONCLUSION
We report a case of PTB treated successfully with carrimycin with APL that requires chemotherapy.
Effect of a brief psychological intervention for common mental disorders on HIV viral suppression: A non-randomised controlled study of the Friendship Bench in Zimbabwe
Authors: Simms Victoria , Abas Melanie A. , Müller Monika , Munetsi Epiphania , Dzapasi Lloyd , Weiss Helen A. , Chibanda Dixon 
Published: January 2024

Background
For people living with co-morbid HIV and common mental disorders (CMD), it is not known whether a brief psychological intervention for CMD can improve HIV viral suppression.

Methods
We conducted a prospective cohort study in eight primary care clinics in Harare, Zimbabwe, enrolling adults with co-morbid HIV and CMD. Six clinics provided the Friendship Bench (FB), a brief psychological intervention for CMD based on problem-solving therapy, delivered by lay counsellors. Two clinics provided enhanced usual care (EUC). The primary outcome was viral non-suppression after six months (viral load ≥400 copies/mL). Data were analysed using a difference-in-difference approach with linear regression of cluster-level proportions, adjusted for baseline viral non-suppression (aDiD). The secondary outcome was presence of CMD measured by the Shona Symptom Questionnaire.

Results
In FB clinics, 407/500 (81.4%) participants had viral load results at baseline and endline: 58 (14.3%) had viral non-suppression at baseline and 41 (10.1%) at endline. In EUC clinics, 172/200 (86.0%) had viral load results at baseline and endline: 22 (12.8%) were non-suppressed at baseline and 26 (15.1%) at endline (aDiD = -7.3%; 95%CI 14.7% to -0.01%; p = 0.05). Of the 499 participants virally suppressed at baseline, the FB group had lower prevalence of non-suppression at endline compared to the EUC group (2.9% vs 9.3%; p = 0.002). There was no evidence of a difference in endline viral non-suppression by group among the 80 participants with non-suppression at baseline (53.5% vs 54.6%; p = 0.93). The FB group was less likely to screen positive for CMD at endline than the EUC group (aDiD = -21.6%; 95%CI -36.5% to -6.7%; p = 0.008).

Conclusion
People living with co-morbid HIV and CMD may benefit from receiving a low-cost mental health intervention to enhance viral suppression, especially if they are already virally suppressed. Research is needed to understand if additional adherence counselling could further improve viral suppression.
Video games and Facebook addiction among Tunisian adolescents: prevalence and associated factors
Authors: Amara Amel , Omri Nihel , Limam Manel , Bannour Rania , Mellouli Menel , Ghardallou Mariem , Zedini Chekib , Sahli Jihene , Mtiraoui Ali 
Published: January 2024

Abstract

Objectives
As social media and online gaming technology have developed very rapidly over the last two decades, their paired issues are of growing concern worldwide. The aim of our study was to assess the prevalence of video game and Facebook addiction and its predictive factors among Tunisian in school adolescents.

Methods
We conducted a cross-sectional study among in school adolescents in Sousse, Tunisia, over the first academic term of the 2019–2020 school year using a self-administered questionnaire to a randomly selected representative sample of 1,353 students. The valid Arabic version of the Bergen Facebook Addiction Scale (BFAS), the Video Game Addiction Scale (GAS-7), the Beck Depression Inventory Short Form (13 items) and the Screen for Child Anxiety Related Disorders (SCARED-C) were used to assess facebook addiction disorder (FAD), internet gaming disorder (IGD), depression and anxiety respectively.

Results
More than one in four pupils (26.1 %; CI 95 %: 23.8–28.4 %) were identified as problematic video game users and almost a third of participants (32.5 %; CI 95 %: 30–35 %) reported FAD. IGD was significantly associated with FAD (p<0.001). Likewise, pupils exhibiting depressive symptoms as well as those with anxiety symptoms were significantly more likely to be problematic video games and Facebook users (p<0.001 for each). Being addicted to Facebook (AOR=1.83, 95 % CI: 1.18–2.81), experiencing anxiety disorders (AOR=2.43, 95 % CI: 1.52–3.88), being male (AOR=2.51, 95 % CI: 1.95–3.23) and spending more than 4 h per day surfing the net (AOR=2.55, 95 % CI: 1.65–3.96) were the determinants of IGD in the multivariate analysis. Similarly, being addicted to video games (AOR=1.82, 95 % CI: 1.21–2.73), experiencing anxiety disorders (AOR=1.85, 95 % CI: 1.20–2.86), having experienced academic failure (AOR=1.73, 95 % CI: 1.16–2.56), and spending more than 4 h per day on social media (AOR=3.75, 95 % CI: 2.38–5.90) were the predictors of FAD.

Conclusions
The prevalence of problematic use of video games and Facebook is alarmingly high. Identifying risk factors can help screen high-risk adolescents. We need additional prevention measures addressing not just adolescents, but all risk factors.
“Looking like a boarding school student”: the construction of unequal personhood in language policy in education
Authors: Phyak Prem 
Published: January 2024

Abstract
The purpose of this article is to analyze the construction of unequal personhood in the institutional logics for the implementation of English as a medium of instruction (EMI) policy. I build on the theories of figures of personhood and figured worlds to discuss how institutions (public schools) use multiple semiotic resources to characterize students’ diverse personality traits that reproduce neoliberal subjectivities shaping their EMI policies. The data for this article are drawn from ethnographic observations and interviews with the teachers from two Nepali public schools that have recently introduced a segregated EMI policy. The analysis of data shows that EMI schools use ‘śikṣita’, ‘sabhya’ and ‘yogya’ personality traits to justify the relevance of EMI policy to produce the educated person. The construction of such person types is shaped by sociocultural and political-economic ideologies and build unequal personhood, reinforcing neoliberal subjecthood and epistemic injustice. My recommendation is that we need to pay attention to examining how language policies in education construct unequal personhood by assigning, imposing, and imaging discriminatory personality traits which remain as the foundation of social injustice.
Discursive formation of personalities: life trajectories of a transnational doctoral student between the UK and China
Authors: Shi Yu (Aimee) 
Published: January 2024

Abstract
Contributing to existing studies on global circuits of knowledge and labour, this paper presents a case study of Andy – a Chinese doctoral student in the UK – and looks at his transition from higher education to later joining the labour market after returning to China through the lens of personality traits. It draws upon literature on language socialisation and sociolinguistic studies of mobility, focusing on how social actors navigate transnational higher education and the consequences on professional development in a neoliberalising market economy. It aims to investigate how (in)appropriate personhood – as manifested in recurrent personality attributes – is enacted and negotiated in specific learning contexts in Andy’s trajectory and the impact on employability, with various types of data consisting of participant observation, interviews, and relevant materials. The analysis suggests that Andy has been trained to engage communicatively with academic tasks in higher education settings. However, Andy considers himself lacking certain personality variables that could align with the criteria of a “good doctoral student” depicted by the institutions, such as being independent, motivated, and self-disciplined. Andy gradually shows disorientation in an academic career, albeit finding his inadequacy to perform the desired professional personhood in a labour market that values working experience and communication skills over education certification. This process explains why sometimes the expected communicative repertories and training acquired in higher education are not transferable into valuable resources that Andy can mobilise to become employed. This paper argues that neoliberal rationality has stratifying effects on individuals primarily due to an emphasis on self-responsibility and constant improvements. In transnational higher education, certain personality traits are considered desirable and lacking such characteristics can have side effects on mobile actors like Andy, who navigate the globalising labour market through uncertainty and precarity.