bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Race Against Time: Evaluating the Speed of Gene Panel Result Delivery in NSCLC Patient Management
Authors: Kunimasa Kei , Tamiya Motohiro , Inoue Takako , Kawamura Takahisa , Miyazaki Akito , Kanzaki Ryu , Maniwa Tomohiro , Honma Keiichiro , Okami Jiro , Nishino Kazumi 
Published: November 2023

Abstract
Background
In this study, we aimed to compare the clinical utility of the Oncomine Dx® Target Test (Oncomine) with that of the AMOY Dx® Pan Lung Cancer PCR panel (AMOY), focusing on their turn-around times (TATs).
Methods
Data on the specimens, fresh-frozen (FF) or formalin-fixed paraffin-embedded (FFPE) tissue samples, the success rate and detected driver alterations, submitted for these gene panel analyses were also collected from medical records. Further, for the comparison of the two methods, TATs defined as: T1, the period from first visit to specimen submission at an external laboratory; T2, the period from first visit to when the attending physician ordered the gene panel test; and T3, the period from first visit until the availability of the analysis results, were measured.
Results
In total, 213 patients, with 157 and 56 who submitted specimens for Oncomine and AMOY, respectively, were enrolled. The success rate of the analyses were 98.0% for Oncomine and 100.0% for AMOY. Further, the detection rates of driver alterations were 57.9% and 50.9% for Oncomine and AMOY, respectively, and 95.9% specimens submitted for Oncomine were FFPE, while 78.2% of those submitted for AMOY were FF. Further, the TATs, T1, T2, and T3, tended to be significantly shorter in AMOY cases. Specifically, the median TAT for AMOY cases, particularly T3, was 12 days (range, 4–26 days).
Conclusions
AMOY showed shorter TATs and a higher success rate than Oncomine. Therefore, its application using FF specimens may be effectively utilized in oncologic emergencies.
Towards Effective Smartphone Pregnancy Apps: A Critical Evaluation of Quality, content and suitability in Freely Available Persian Pregnancy Apps
Authors: Asadollahi Fateme , Ebrahimzade samira , Roudsari Robab Latifnejad 
Published: November 2023

Abstract
Background
The rise of health apps in the digital health landscape has created opportunities for health promotion, particularly in pregnancy. However, despite pregnancy mobile apps' wide distribution and popularity, there is limited data on their quality and content. This review aimed to evaluate the Quality, content, and suitability of the most popular and freely available Persian smartphone pregnancy-related apps.
Methods
163 apps were screened from Google Play and Cafebazaar app store using the search term pregnancy app until July 2023. Apps in the Farsi language, freely available, downloaded more than 10,000 times, and designed for pregnant women were included. Finally, only nine apps met the inclusion criteria, downloaded on mobile phones and assessed by two independent reviewers using the Mobile App Rating Scale (MARS), the Coverage and Depth of Information Scale, and the Suitability Assessment of Materials (SAM).
Results
The mean of the four MARS subscale scores across all nine apps was 3.55 ± 0.61 out of a maximum of 5. Aesthetics and functionality subscales were rated the highest, followed by engagement and information subscales. Overall, 66% of apps were rated poor for coverage and depth of health information, 33% as adequate, and no app received a superior level. Regarding SAM, 44% of apps were rated superior, 55% adequate, and no app received a poor score.
Conclusions
This evaluation highlights the importance of evaluating the quality and content of pregnancy apps before recommending them to pregnant women. Healthcare providers should suggest high-quality pregnancy apps with appropriate content for pregnant women.
Simultaneous Production of Vitamin K2 and Iturin A by Bacillus velezensis ND
Authors: Shen Yeqiao , Cao Xiaojie , Tang Zhongmin , Gao Yuqiu , Zhang Huili 
Published: November 2023

Abstract
Bacillus velezensis is known to produce a diverse array of secondary metabolites, including the ability to synthesize lipopeptides such as iturin A, and additionally produce higher amounts of vitamin K2. Consequently, we sought to employ co-production fermentation to simultaneously generate vitamin K2 and iturin A. In order to significantly reduce fermentation costs and enhance substrate utilization efficiency, this article initially established a straightforward and effective product extraction method that enables the separate collection of vitamin K2 and iturin A from the fermentation broth. Simultaneously, the impacts of glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium, employed as carbon and nitrogen sources in the culture medium, on the co-production of vitamin K2 and iturin A were refined by optimizing factors such as culture temperature, initial pH and the concentrations of trace elements Na+ and K+, as well as additional additives L-glutamic acid sodium. It was discerned that glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium significantly influenced the concurrent production of vitamin K2 and iturin A. As a result, a novel process for the efficient co-production fermentation of vitamin K2 and iturin A was established, thereby enhancing the value-added aspect of the fermentation process. Furthermore, the implementation of a two-compartment biofilm reactor synergistically elevated the co-production efficiency. The yields of vitamin K2 and iturin A reached 46.88 mg/L and 5.58 g/L, respectively. This study provides a theoretical framework and practical implications for the integrated double-product fermentation process.
Innovative, simple and efficient algorithms for logarithms with adjustable accuracy
Authors: Gepner Paweł , Moroz Leonid , Samotyy Volodymyr , Kokosiński Zbigniew , Gavkalova Nataliia , Kocot Bartłomiej 
Published: November 2023

Abstract
The computation of logarithmic functions is essential for many computational applications in science and engineering. Various computational techniques have been developed to approximate these functions. In this article, we present a simple and efficient algorithm for calculating the logarithmic function using IEEE 754 standard arithmetic types that minimises the number of arithmetic operations compared to previous implementations. Our starting point is Mitchell’s linear approximation, which reduces the computation of the logarithm to just the addition of the exponent and the significand. We developed a parameterised framework for choosing the number of segments in a piecewise polynomial approximation scheme in combination with polynomial degree and bit manipulation techniques, which allows a tradeoff between the accuracy and efficiency. We measured a speedup by a factor of 3--20 with respect to the C/C++ library software implementation while keeping the absolute error at a low level. This article presents and discusses our proposed implementation, compares it to other logarithm realisations and tests these realisations on various platforms.
Repetitive mild traumatic brain injury elicits a reactive microglial morphology and elevates serum neurofilament light levels, independent of NLRP3 inflammasome inhibition
Authors: O'Brien William , Green Tabitha R.F. , Pham Louise , Bain Jesse , Sobey Christopher G , Robertson Avril A B , O’Brien Terence J. , Monif Mastura , Shultz Sandy R , Rowe Rachel K. , McDonald Stuart J. 
Published: November 2023

Abstract
There is growing evidence that a second mild traumatic brain injury (mTBI) sustained prior to cellular and neurological recovery from the first increases risk of cumulative pathological and neurological deficits. Despite this, the pathophysiology of single and repeated mTBI (rmTBI) is not well understood, and as such, no therapeutic interventions are available to mitigate the cumulative deficits induced. The Nod-like receptor pyrin domain containing 3 (NLRP3) inflammasome is an intracellular protein complex that is critical to the innate immune response. Although neuroinflammation is a key feature of mTBI pathophysiology, little is known about the role of the inflammasome in rmTBI. To investigate this, we used a clinically relevant rat model of mTBI and assessed whether pharmacologically inhibiting NLRP3 with the specific NRLP3 inhibitor MCC950, reduced inflammasome activation in the brain and thereby improved acute behavioural and molecular outcomes. We hypothesised that rmTBI would result in up-regulation of inflammasome-associated genes, a reactive microglial phenotype, axonal injury, and neurobehavioural deficits, and that these factors would be attenuated by NLRP3 inflammasome inhibition with MCC950. We found that rmTBI increased hippocampal apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mRNA expression, increased microglia cell number in the motor cortex, sensorimotor cortex, hippocampus, corpus callosum and thalamus, and elevated serum neurofilament light (NfL) levels that correlated with both the number of microglia in the corpus callosum, and sensorimotor deficits. These changes were not mitigated by MCC950 treatment. This study provides evidence that the NLRP3 inflammasome does not play a significant role in the acute neuroinflammatory response following rmTBI; however further studies are required to investigate its role chronically.
Clinical Efficacy and Central Mechanisms of Electroacupuncture Treatment for Low Back Pain in Primary Osteoporosis: Study Protocol for a Randomized Controlled Trial
Authors: Huang Chihuan , Liu Jing , Wang Zun , Zhang Di , Guo Zhijie , Wei Liyu , Liu Shaowei , Liu Lanying , Xu Daoming 
Published: November 2023

Abstract
Background
Primary osteoporosis is a common disease in the elderly, often causing low back pain and limited mobility. Electroacupuncture is considered a combination of traditional acupuncture and modern transcutaneous electrical nerve stimulation. Due to its strong stimulation, it has shown certain efficacy in relieving low back pain associated with primary osteoporosis (POP). It has the potential to be a complementary or alternative treatment for drug therapy of POP-related low back pain. However, the underlying mechanisms have not been fully elucidated. Therefore, this study, by evaluating the efficacy of electroacupuncture in treating POP-related low back pain, aims to explore the potential central mechanisms using resting-state functional magnetic resonance imaging (rs-fMRI).
Methods
This study is a randomized controlled trial in which 60 patients were randomly divided into two groups: the calcium group (n = 30) and the calcium + electroacupuncture group (n = 30). The study lasted for 17 weeks, including a 1-week baseline, 4 weeks of treatment, and 12 weeks of follow-up (at the 4th and 12th week after treatment). The primary outcome measure is McGill Pain Questionnaire (MPQ), and secondary outcome measures include the Oswestry dysfunction index(ODI), Young's Modulus, and the use of analgesic medications after treatment. These measures will be used to evaluate the clinical efficacy of the two intervention methods for treating low back pain associated with primary osteoporosis (POP). Twenty patients from each group will be randomly selected for rs-fMRI examinations to observe changes in brain functional activity and correlate them with changes in pain severity.
Discussion
This study aims to evaluate the clinical efficacy and potential central mechanisms of electroacupuncture in treating low back pain associated with POP. Provided that we obtain imaging-based evidence of the effectiveness of electroacupuncture intervention for POP-related low back pain, this study will provide supporting evidence for the clinical application of electroacupuncture in the treatment of low back pain associated with POP.
Trial registration:
Chinese Clinical Trial Registry ChiCTR2300075736. Registered on 14 September 2023
Assessing the Value of Disulfide Death-Associated Genes in the Prognosis and Treatment of Glioma
Authors: Wu HaoYuan , Yang ZhiHao , Chang ChenXi , Wang ZhiWei , Zhang DeRan , Guo QingGuo , Zhao Bing 
Published: November 2023

Abstract

Background
"Disulfide death," a form of cellular demise, is triggered by the abnormal accumulation of intracellular disulfides under conditions of glucose deprivation. However, its role in the prognosis of glioma remains undetermined. Therefore, the main objective of this study is to establish prognostic signature based on disulfide death-related genes and to provide new solutions in choosing the effective treatment of glioma.
Methods
The combined analysis utilizes data from the Atlas of Cancer Genome (TCGA), the Chinese Glioma Genome Atlas (CGGA), and the Genotype-Tissue Expression (GTEx) databases to screen disulfide death-related genes. Then, TCGA and CGGA serve to construct and verify prognostic signature. In addition, the immunological characteristics of the model and the potential predictive value of immune and chemotherapeutic responses were further explored. Finally, the effect of Importin-4(IPO4) on gliomas has been further confirmed through functional experiments.
Results
7 genes associated with disulfide death were obtained and two subgroups of patients with different prognosis and clinical characteristics were identified. Risk signature was subsequently developed and proved to serve as an independent predictor. The high-risk group exhibited immunosuppressive microenvironment, and was characterized by high concentration of M2 macrophages and regulatory T cells (Tregs). Patients in high-risk group may benefit more from immunotherapy and chemotherapy. In addition, in vitro experiments have shown that inhibition of the expression of IPO4 leads to a significant reduction in the proliferation, migration, and invasion of glioma cells.
Conclusion
This study constructed the first prognostic disulfide death-related gene signature in glioma with the ability to optimize the choice of patients receiving immuno- and chemotherapies and provided a potential therapeutic target for glioma.
200 years of taxonomic confusion: Sporendonema and allies
Authors: Kandemir Hazal , Decock Cony , Hernández-Restrepo Margarita , Labuda Roman , Houbraken Jos , Ilkit Macit , Hoog GS Sybren 
Published: November 2023

Abstract
The genus Sporendonema was introduced in 1827 with the type species S. casei. for a red mold on cheese. Cheese is a consistent niche for this species. Sphaerosporium equinum is another species classified in Gymnoascaceae (Onygenales) and has also been reported from cheese. Recently, other habitats have been reported for both Sporendonema casei and Sphaerosporium equinum. The present study aimed to investigate the taxonomy of Sporendonema and Sphaerosporium, as well as a close neighbor, Arachniotus. Hormiscium aurantiacum, another related cheese-associated species was also included in the analyses. Strains were evaluated in terms of colony morphology, micromorphology, salt tolerance, growth rate at different temperatures, casein degradation, cellulase activity and multilocus phylogeny with sequences of the nuclear ribosomal internal transcribed spacer region, the D1-D2 region of the large subunit and partial β-tubulin locus sequences. The results showed that the analysed species were congeneric, and the generic names Arachniotus and Sphaerosporium should be reduced to the synonymy of Sporendonema. Therefore, four new combinations as well as one lectotype and one epitype were designated in Sporendonema. Two strains attributed to Sphaerosporium equinum from substrates other than cheese were found to be phylogenetically and morphologically deviant and were introduced as a new species named Sporendonema isthmoides.
Knowledge Graph Completion Model based on Hyperbolic Hierarchical Attention Network
Authors: Luo Jiaohuang , Song Changlong 
Published: November 2023

Abstract
In Knowledge Graph Completion (KGC), missing knowledge triples are inferred based on known facts in the knowledge base. In recent years, several representation learning models for knowledge reasoning have achieved promising link prediction results, especially those based on graph attention networks and their derivatives. Such models typically leverage local neighborhood information for each node to learn representation vectors of target entities. However, existing knowledge representation models solely focus on entity embeddings and employ straightforward linear transformations to update relationships. Hence, they are unable to effectively explore structured information with different semantics at the entity and relationship levels. To address these issues, a novel Hyper-bolic Hierarchical Attention Network model for Knowledge Graph Completion (HHAN-KGC), is proposed, incorporating the inference capabilities of hyperbolic algorithms for structured data and the learning capabilities of hierarchical attention networks for entities and relationships, jointly extracting feature vectors for entity and relationship layers. The hyperbolic hierarchical attention network consists of a dual-layer attention mechanism and a relationship semantics mechanism , designed to deeply explore hierarchical feature information embedded in local structures. Thus, HHAN-KGC can establish close associations among triples at different semantic levels, ensuring that the learned knowledge representations possess a certain degree of fidelity and effectively aggregate structured information from entity and relationship layers. Experimental results on multiple knowledge graph datasets demonstrate that HHAN-KGC outperforms the current state-of-the-art methods for knowledge graph link prediction.
Management of incessant Ventricular Arrhythmias in a Patient with left ventricular assist device: a case report
Authors: Chen Chen , Du Juan , Wang Xianqiang , Zou Liang 
Published: November 2023

Abstract
Background
The implementation of left ventricular assist devices (LVADs) as a bridge to transplantation or as destination therapy in end-stage heart failure patients is frequently complicated by the emergence of ventricular arrhythmias (VAs). These arrhythmias have been implicated in precipitating deleterious clinical outcomes, increased mortality rates and augmented healthcare expenditures. The strategic management of VAs in the context of LVAD support is a critical area of ongoing research.
Case Presentation:
We present a challenging case of a 49-year-old male with a history of dilated cardiomyopathy who received an LVAD. Post-implantation, the patient suffered from intractable VAs, leading to multiple rehospitalizations and hemodynamic deterioration. Despite exhaustive medical management and electrical cardioversion attempts, the patient's VAs persisted, ultimately necessitating prioritization for cardiac transplantation.
Discussion
This case highlights the challenges in managing VAs in LVAD patients and the importance of multidisciplinary collaboration. While pharmacological intervention is the initial strategy, catheter ablation may be considered in selected cases when medication is insufficient. In instances of intractable VAs, expeditious listing for heart transplantation as a high-priority candidate is advisable when feasible.
Sitting foot: Posture dependent changes of volume, edema and perfusion of the foot. A prospective interventional study with 27 volunteers
Authors: Freund Wolfgang , Wikstroem Peter , Wunderlich Arthur P , Schuetz Uwe , Beer Meinrad 
Published: November 2023

Abstract

Background
Sitting is known to be bad for your cardiovascular health. We furthermore hypothesized that sitting posture will reduce perfusion of the foot and increase edema, possibly predisposing to disease like osteochondritis.
Methods
We included 27 healthy volunteers and performed MRI measurements including arterial spin labelling (ASL) and intravoxel incoherent motion (IVIM) perfusion as well as short tau inversion recovery (STIR) edema measurement and 3D volumetry. After randomization, the elevation of one foot during the day was used as an intervention.
Results
Intra- and interrater variability was 1-6%.
ASL perfusion measurement was hindered by artifacts. IVIM perfusion showed no significant changes during supine measurements.
Volumetry could demonstrate a highly significant (p=0.00005) volume increase, while the intervention led to a significant (p=0.0076) volume decrease during the day. However, the water content in STIR remained unchanged and the normalized (quotient bone/muscle) edema was reduced on the control side (p=0.006) and increased on the intervention side (p=0.01).
Conclusions
Sitting all day leads to swelling of the healthy foot. Compensation in healthy subjects seems to prevent lasting perfusion changes or edema evolution in the bone despite an increase of muscle signal and volume increase. Thus, the etiology of osteochondritis needs further studies.
Enhanced CT, DTI, and 1H-MRS Assisted Robot-Guided Brain Glioma Biopsy: A Single-Center Study
Authors: Liao Changpin , Cen Jian , Li Xiaolei , Li Zhonghua , NORDIN RUSLI BIN , Li Tingyang , Ye Jing , Huang Lide , Wei Wei , Wei Xianfu , Yang Haiyan , Chen Wu , Han Jianglong 
Published: November 2023

Abstract
Objective
To explore the positive rate of pathological results and safety of enhanced CT, DTI, and 1H-MRS assisted robot-guided brain glioma biopsy.
Methods
Eighteen patients with imaging-diagnosed brain gliomas who underwent robot-guided brain glioma biopsy in the Department of Neurosurgery of Baise People's Hospital from January 2021 to October 2022 were selected as the study subjects. All patients underwent preoperative examinations such as enhanced CT, DTI, and 1H-MRS. The neurosurgical robot Reme-Studio automatically fused the image pictures, using the principles of enhanced CT vascular imaging points, DTI reconstructed conduction bundle three-dimensional stereograms, and 1H-MRS indicating the tumor cells, to perform robot-guided brain glioma biopsy. The specimens were sent to the pathology department for routine pathological results, and the positive rate of specimens and postoperative complications were recorded.
Results
All 18 patients obtained clear pathological grades, and no intracranial bleeding requiring emergency surgery or new-onset neurological dysfunction occurred after surgery.
Conclusion
Enhanced CT, DTI, and 1H-MRS assisted robot-guided brain glioma biopsy can accurately locate and puncture the metabolic hotspots of the edema zone, marginal zone, and tumor body CHo/NAA maximum value, avoiding important blood vessels and conduction bundles. It is not only an important method to improve the positive rate of pathological results but also safe and reliable.