bluuebunny/crossref_metadata_2025_split huggingface dataset:
CO2‐Berechnungen von Brücken mit Bauwerkslängen bis 40 m
Authors: Görtz Stephan , Pham Thi Kim Dung 
Published: January 2024

AbstractIm Rahmen eines von der Gesellschaft für Energie und Klimaschutz Schleswig‐Holstein GmbH (EKSH) geförderten Forschungsvorhabens wurden CO2‐Blanzierungen an insgesamt 34 realen und mehreren Hundert generierten Brückenbauwerken durchgeführt. Zielsetzung war es, Erfahrungswerte zu erarbeiten, bei welchen Brückenkonstruktionen wie viel CO2 emittiert wird, und Vorschläge auszuarbeiten, wie sich die CO2‐Emissionen reduzieren lassen.
Inulin alters gut microbiota to alleviate post‐stroke depressive‐like behavior associated with the IGF‐1‐mediated MAPK signaling pathway
Authors: Shao Rong , Tan Xiongchang , Pan Minfu , Huang Jiawen , Huang Liu , Bi Binyu , Huang Xiaohua , Wang Jie , Li Xuebin 
Published: January 2024

AbstractIntroductionGut microbiota dysbiosis is a key factor of the pathogenesis of post‐stroke depression (PSD). PSD is associated with increased hippocampal neuronal apoptosis and decreased synaptic connectivity. Inulin can be involved in hippocampal neuron protection through the microbiome–gut–brain axis. However, the neuroprotective effects of inulin in PSD are still to be further investigated.MethodsBy utilizing the GEO public database, we identify differentially expressed genes in the hippocampus following inulin intake. This can help us discover key signaling pathways through functional enrichment analysis. Furthermore, we validate the expression levels of signaling molecules in a rat model of PSD and examine the effects of inulin on behavioral changes and body weight. Additionally, conducting a microbiome analysis to identify significantly different microbial populations and perform correlation analysis.ResultsThe intake of inulin significantly up‐regulated mitogen‐activated protein kinase signaling pathway in the hippocampus. Inulin changed in the gut microbiota structure, leading to an increase in the abundance of Lactobacillus and Clostridium_sensu_stricto_1 in the intestines of PSD rats, while decreasing the abundance of Ruminococcus UCG_005, Prevotella_9, Oscillospiraceae, and Clostridia UCG_014. Furthermore, the inulin diet elevated levels of insulin‐like growth factor 1 in the serum, which showed a positive correlation with the abundance of Lactobacillus. Notably, the consumption of inulin‐enriched diet increased activity levels and preference for sugar water in PSD rats, while also reducing body weight.ConclusionThese findings highlight the potential therapeutic benefits of inulin in the management of depression and emphasize the importance of maintaining a healthy gut microbiota for PSD.
Highly efficient CRISPR/Cas9‐mediated exon skipping for recessive dystrophic epidermolysis bullosa
Authors: du Rand Alex , Hunt John , Samson Christopher , Loef Evert , Malhi Chloe , Meidinger Sarah , Chen Chun‐Jen Jennifer , Nutsford Ashley , Taylor John , Dunbar Rod , Purvis Diana , Feisst Vaughan , Sheppard Hilary 
Published: January 2024

AbstractGene therapy based on the CRISPR/Cas9 system has emerged as a promising strategy for treating the monogenic fragile skin disorder recessive dystrophic epidermolysis bullosa (RDEB). With this approach problematic wounds could be grafted with gene edited, patient‐specific skin equivalents. Precise gene editing using homology‐directed repair (HDR) is the ultimate goal, however low efficiencies have hindered progress. Reframing strategies based on highly efficient non‐homologous end joining (NHEJ) repair aimed at excising dispensable, mutation‐harboring exons offer a promising alternative approach for restoring the COL7A1 open reading frame. To this end, we employed an exon skipping strategy using dual single guide RNA (sgRNA)/Cas9 ribonucleoproteins (RNPs) targeted at three novel COL7A1 exons (31, 68, and 109) containing pathogenic heterozygous mutations, and achieved exon deletion rates of up to 95%. Deletion of exon 31 in both primary human RDEB keratinocytes and fibroblasts resulted in the restoration of type VII collagen (C7), leading to increased cellular adhesion in vitro and accurate C7 deposition at the dermal‐epidermal junction in a 3D skin model. Taken together, we extend the list of COL7A1 exons amenable to therapeutic deletion. As an incidental finding, we find that long‐read Nanopore sequencing detected large on‐target structural variants comprised of deletions up to >5 kb at a frequency of ~10%. Although this frequency may be acceptable given the high rates of intended editing outcomes, our data demonstrate that standard short‐read sequencing may underestimate the full range of unexpected Cas9‐mediated editing events.
The augmentation of cytotoxic immune cell functionality through physical exertion bolsters the potency of chemotherapy in models of mammary carcinoma
Authors: Qin Bingqing , He Zhongshi , Xie Lixia , Feng Shenglan , Ye Junjie , Gui Jianjun , Sun Xiaodong , Sang Ming 
Published: January 2024

AbstractBackgroundMammary carcinoma, a pervasive and potentially lethal affliction, is conjectured to be profoundly influenced by physical exercise, both in prophylaxis and therapeutic contexts. This study endeavors to explore the repercussions of exercise training on the progression of mammary carcinoma, particularly the mechanisms by which the amalgamation of an exercise regimen and doxorubicin induces tumor cell apoptosis.MethodsFemale BALB/c mice were categorized into four distinct groups: A sedentary group (SED), an exercise group (Ex), a doxorubicin group (Dox, 5 mg/kg), and a combined treatment group (Dox + Ex). The exercise training lasted for 21 days and included aerobic rotarod exercise and resistance training. The impact of exercise training on tumor growth, immune cell proportions, inflammatory factor levels, and cell apoptosis pathway was assessed.ResultsExercise training significantly curtailed tumor growth in a mouse model of breast cancer. Both the Ex and Dox groups exhibited significant reductions in tumor volume and weight (p < 0.01) in comparison to the SED group, while the Dox + Ex group had a significantly lower tumor volume and weight than the Dox group (p < 0.01). Exercise training also significantly increased the proportion of NK and T cells in various parts of the body and tumor tissue, while decreasing tumor blood vessels density. Exercise training also increased IL‐6 and IL‐15 levels in the blood and altered the expression of apoptosis‐related proteins in tumor tissue, with the combined treatment group showing even more significant changes.ConclusionsPhysical training improves the effectiveness of doxorubicin in treating breast cancer by activating cytotoxic immune cells, releasing tumor suppressor factors, and initiating mt‐apoptosis, all while mitigating the adverse effects of chemotherapy.
Chitosan/alginate scaffold enhanced with Berberis vulgaris extract for osteocyte differentiation of ovine fetal stem cells
Authors: Soltani Leila , Ghaneialvar Hori , Abbasi Naser , Bayat Parvaneh , Nazari Maryam 
Published: January 2024

AbstractDesigning biocompatible polymers using plant derivatives can be extremely useful in tissue engineering, nanomedicine, and many other fields of medicine. In this study, it was first looked into how chitosan/alginate scaffolds were made and characterized in the presence of berberine and barberry fruit extract. Second, the process of proliferation and differentiation of ovine fetal BM‐MSCs (bone marrow‐mesenchymal stem cells) was assessed on these scaffolds after BM‐MSCs were extracted and confirmed by developing into osteocyte and adipose cells. To investigate the differentiation, treatment groups include (1) ovine fetal BM‐MSCs were plated in Dulbecco's modified eagle medium culture medium with high glucose containing 10% fetal bovine serum and antibiotics (negative control), (2) ovine fetal BM‐MSCs were plated in osteogenic differentiation medium (positive control group), (3) positive control group + barberry fruit extract, (4) positive control group + berberine, (5) ovine fetal BM‐MSCs were plated in osteogenic differentiation medium on chitosan/alginate scaffold (hydrogel group), (6) ovine fetal BM‐MSCs were plated in osteogenic differentiation medium on chitosan/alginate/barberry fruit extract scaffold (hydrogel group containing barberry fruit extract), and (7) ovine fetal BM‐MSCs were plated in osteogenic differentiation medium on chitosan/alginate/berberine scaffold (hydrogel group containing berberine). Alkaline phosphatase (ALP) enzyme concentrations, mineralization rate using a calcium kit, and mineralization measurement by alizarin staining quantification were all found after 21 days of culture. In addition, real‐time quantitative reverse transcription polymerase chain reaction was used to assess the expression of the ALP, COL1A2, and Runx2 genes. Days 5 and 7 had the lowest water absorption by the hydrogel scaffold containing barberry extract, which was significant in comparison to other groups (p < .05). Among the hydrogel scaffolds under study, the one containing barberry extract exhibited the lowest tensile strength, and this difference was statistically significant (p < .05). The chitosan/alginate hydrogel has the highest tensile strength of all of them. In comparison to the control and other treatment groups, the inclusion of berberine in the chitosan/alginate hydrogel significantly increased the expression of the ALP, Runx2, and COL1A2 genes (p < .05). The osteocyte differentiation of mesenchymal stem cells in in vitro settings appears to have been enhanced by the inclusion of berberine in the chitosan/alginate scaffold.
Investigation on a compact wideband omnidirectional circularly polarized antenna in an indoor propagation channel for 2.4 GHz application
Authors: Emadian Seyed Ramin , Ghobadi Changiz 
Published: January 2024

SummaryIn this paper, for the first time, the capability of a compact low‐cost wideband omnidirectional circularly polarized (CP) antenna to suppress multipath fading characteristics in a typical indoor propagation channel for 2.4 GHz applications is investigated. A ray tracing method based on the geometrical optics and uniform theory of diffraction is applied to analyze an indoor propagation channel using the measured radiation patterns of the proposed antenna. The design uses a conventional monopole antenna with a circular ground plane to generate a vertically polarized electric field. Sequentially flag‐shaped stubs are then added to the ground plane to produce horizontally polarized electric field and to improve the impedance bandwidth of the proposed antenna. The antenna is designed for 2.4 GHz WLAN application with 10 dB impedance bandwidth of 25.5% and 3 dB axial ratio (AR) bandwidth of 24.5%. The polarization can be readily changed between the left‐hand circular polarization (LHCP) and right‐hand circular polarization (RHCP) by altering the direction of the coaxial probe (monopole) or varying the orientation of the printed bended stubs. Both the LHCP and RHCP prototypes of the proposed antenna are designed, fabricated, and tested, and the similar results are obtained.
New insights into suspended drops: When soft matter meets acoustic levitation
Authors: Chen Hongyue , Hong Zhenyu , Zang Duyang 
Published: January 2024

AbstractAcoustic levitation has developed into a popular but elegant tool for the study of drops as well as soft matter due to its exceptional levitation capabilities to a variety of liquid samples. The acoustically levitated drops offer opportunities for the investigation of a wide range of fundamental issues related to liquid drops. In this review, the unique physics/chemical processes involved in acoustically levitated drops are dealt with. We first introduce the dynamics of the acoustically levitated drops, including drop oscillation, coalescence, and the associated capillary phenomena. The bubble formation and stability are also discussed. Depending on the inhibition of solid surfaces and the nonlinear effects of ultrasound, the self‐assembly of colloidal particles at the air–liquid interface as well as granular matter in air is reviewed. In particular, the exploration of biological drops by using acoustic levitation is also highlighted. In the end, the concept of acoustic‐levitation‐fluidics and possible potential topics are proposed.
Supercapacitor using polyvinyl alcohol doped ionic liquid 1‐butyl‐1‐methylpyrrolidinium hexafluorophosphate polymer electrolyte system
Authors: Kanika  , Gairola Yashvi , Tomar Richa , Kumar Sushant , Konwar Subhrajit , Savilov Serguei V. , Yadav Tarun , Yahya M. Z. A. , Singh Pramod K. 
Published: January 2024

AbstractPolyvinyl alcohol‐based polymeric films with NaI and ionic liquid (1‐Butyl‐1‐methylpyrrolidinium hexafluorophosphate) have been developed by using the solution cast method. The produced polymer electrolyte sheets were evaluated using various methods. Electrochemical impedance spectroscopy was utilized to measure the conductivity of the polymeric films, and the ionic liquid doped films showed an increase with a maximum at 6 wt% ionic liquid with a value for conductivity of 4.545 × 10−4 S cm−1. The morphological features of the most conductive polymer were further investigated using polarized optical microscopy and x‐ray diffraction. Ionic doping causes an increase in the amorphous nature. Using Fourier transform infrared spectroscopy, an excellent complexity between PVA, salt NaI, and ionic liquid was assessed. Thermogravimetric analysis was used to inspect the polymer films' thermal stability. The highest conducting sample was used for manufacturing an electric double‐layer capacitor. The behavior of manufactured electric double‐layer capacitor has been evaluated through EIS, CV, and GCD.
Comparison of the AquaCrop and CERES‐Maize models for simulating maize phenology, grain yield, evapotranspiration and water productivity under different irrigation and nitrogen levels
Authors: Amiri Ebrahim , Irmak Suat , Tari Davood Barari 
Published: January 2024

AbstractThe CERES‐Maize and AquaCrop models were evaluated for their performance in simulating maize (Zea mays L.) phenology, evapotranspiration (ETc), grain yield and crop water productivity (CWP). Model input data for calibration, validation and simulations were obtained from field experiments conducted from 2011 to 2014 that imposed three different irrigation levels (full irrigation treatment [FIT], limited irrigation treatment [75% FIT] and rainfed control) and five nitrogen (N) management treatments. Both models performed well in simulating the maize phenological stages, with simulated values being within 1–4 days of measured values for both models. Both models simulated grain yield well during the calibration with a normalized root mean squared error (RMSEn) of 5% (0.6 t ha⁻1) for CERES‐Maize and 8% (0.80 t ha⁻1) for AquaCrop. The model error for ETc varied from −2 to +14% for CERES‐Maize and ranged from −3 to +11% for AquaCrop. High N levels resulted in higher accuracy in predicting ETc. The AquaCrop model performed better than CERES‐Maize in simulating irrigated crop production under different N levels under rainfed conditions. The performance of both models for rainfed systems was poorer than their performance in irrigated systems, indicating that both models need further improvements in simulating rainfed maize production systems.
Off‐farm employment and body mass index among agrarian women in sub‐Saharan Africa
Authors: Tsiboe Francis , Aseete Paul , Di Fang 
Published: January 2024

AbstractThe relationship between employment and women's weight outcomes in sub‐Saharan Africa (SSA) is less studied. In this study, we use nearest‐neighbor matching to analyze the association between off‐farm employment and women's body mass index (BMI), using data from 364,426 adult women from 36 SSA countries. We find that employment off‐farm is associated with improvement in women's weight status. Off‐farm employment is associated with higher BMI in women (by 1.6%), and lower BMI among obese and overweight women (by 28% and 16%, respectively). These findings provide information that could guide policymakers working at the intersection of women's health and employment.
The prevalence of obstructive sleep apnea syndrome after COVID‐19 infection
Authors: Lin Wen‐Chun , Wu Meng‐Che , Wang Yu‐Hsun , Lin Che‐Hsuan , Wei James Cheng‐Chung 
Published: January 2024

AbstractObstructive sleep apnea is a well‐known risk factor regarding the severity of COVID‐19 infection. However, to date, relatively little research performed on the prevalence of obstructive sleep apnea in COVID‐19 survivors. The purpose of this study was to investigate the risk of obstructive sleep apnea after COVID‐19 infection. This study was based on data collected from the US Collaborative Network in TriNetX. From January 1, 2020 to June 30, 2022, participants who underwent the SARS‐CoV‐2 test were included in the study. Based on their positive or negative results of the COVID‐19 test results (the polymerase chain reaction [PCR] test), we divided the study population into two groups. The duration of follow‐up began when the PCR test was administered and continued for 12 months. Hazard ratios (HRs) and 95% confidence intervals (CIs) for newly recorded COVID‐19 positive subjects for obstructive sleep apnea were calculated using the Cox proportional hazards model and compared to those without COVID‐19 infection. Subgroup analyses were performed for the age, sex, and race, groups. The COVID‐19 group was associated with an increased risk of obstructive sleep apnea, at both 3 months of follow‐up (HR: 1.51, 95% CI: 1.48–1.54), and 1 year of follow‐up (HR: 1.57, 95% CI: 1.55–1.60). Kaplan–Meier curves regarding the risk of obstructive sleep apnea revealed a significant difference of probability between the two cohorts in the follow‐up periods of 3 months and 1 year (Log‐Rank test, p < 0.001). The risks of obstructive sleep apnea among COVID‐19 patients were significant in the less than 65 year of age group (HR: 1.50, 95% CI: 1.47–1.52), as well as in the group older than or equal to 65 years (HR:1.69, 95% CI: 1.64–1.73). Furthermore, the risks of obstructive sleep apnea were evident in both the male and female COVID‐19 groups. Compared to the control group, the risks of obstructive sleep apnea in the COVID‐19 participants increased in the subgroups of White (HR: 1.62, 95% CI: 1.59–1.64), Blacks/African Americans (HR: 1.50, 95% CI: 1.45–1.55), Asian (HR: 1.46, 95% CI: 1.32–1.62) and American Indian/Alaska Native (HR: 1.36, 95% CI: 1.07–1.74). In conclusion, the incidence of new diagnosis obstructive sleep apnea could be substantially higher after COVID‐19 infection than non‐COVID‐19 comparison group. Physicians should evaluate obstructive sleep apnea in patients after COVID‐19 infection to help prevent future long‐term adverse effects from occurring in the future, including cardiovascular and neurovascular disease.
Mucosal antibody response and SARS‐CoV‐2 shedding in patients with COVID‐19 related olfactory dysfunction
Authors: Sharma Shilpee , Thiriard Anaïs , Olislagers Véronique , Lechien Jerome R. , Jurion Marie‐Hélène , Delforge Marie‐Luce , Marchant Arnaud , Saussez Sven 
Published: January 2024

AbstractOlfactory dysfunction (OD) was one of the most common symptom of infection with the Wuhan strain of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and could persist for several months after symptom onset. The pathogenesis of prolonged OD remains poorly understood but probably involves sustained viral replication associated with limited mucosal immune response to the virus. This prospective study was conducted to investigate the potential relationship between nasal SARS‐CoV‐2 viral load and antibody levels in patients with loss of smell. One hundred and five patients were recruited 2 weeks after presenting with confirmed coronavirus disease 2019 associated OD. Based on the identification sniffing test performed at enrollment, 52 patients were still anosmic or hyposmic and 53 were normosmic. SARS‐CoV‐2 was detectable in nasal wash of about 50% of anosmic and normosmic patients. Higher viral load was detected in anosmic patients with lower levels of SARS‐CoV‐2 specific nasal immunoglobulins (Ig) IgG and IgA. This association was not observed in normosmic patients. No relationship between nasal viral load and antibodies to endemic coronaviruses was observed. SARS‐CoV‐2 replication in the nasal cavity may be promoted by defective mucosal antibody responses in patients with OD. Boosting mucosal immunity may limit nasal SARS‐CoV‐2 replication and thereby help in the control of persistent OD.
Chemical ablation of the left ventricular endocardium reduces ventricular fibrillation inducibility in acute ischemic canine heart
Authors: Nitta Takashi , Asano Tetsuo , Yajima Toshimi , Otsuka Toshiaki , Ishii Yosuke 
Published: January 2024

AbstractObjectiveVentricular fibrillation remains as the major cause of death in patients with acute myocardial infarction. Effects of trans‐atrial chemical ablation of the left ventricular (LV) endocardium with Lugol's solution on ventricular fibrillation inducibility and ventricular conduction were examined in canines with acute myocardial ischemia.Materials and MethodsChemical ablation of the LV endocardium with Lugol's solution or normal saline was preformed through a left atrial appendage in 14 canines 30 min after occlusion of the left anterior coronary artery.ResultsVentricular fibrillation threshold decreased after the coronary artery occlusion and increased after endocardial chemical ablation. There was a significant difference in the ventricular fibrillation threshold after chemical ablation between with Lugol's solution and with normal saline (25.9 ± 9.2 mA vs. 11.3 ± 2.7 mA, p < .01). QRS width significantly increased from 88 ±  to 116 ±  (p < .01) after the chemical ablation with Lugol's solution, and the activation map of the ventricles demonstrated a left bundle branch block ventricular conduction pattern. Histological examination of the LV endocardium showed lymphocyte infiltration for a depth of 1 mm.ConclusionsChemical ablation of the LV endocardium with Lugol's solution injures endocardial conduction system and increases ventricular fibrillation threshold in the early phase of myocardial ischemia in canines. The procedure may be useful in suppressing intractable ventricular tachyarrhythmias in patients with acute myocardial ischemia.
An analytical overview of the composition and characteristics of China's food safety standards
Authors: Liu Chen , Wang Nan , Liu Ling‐Xiao , Zhang Ying‐Yuan , Liu Yun‐Guo 
Published: January 2024

AbstractThis paper discusses the framework of China's food safety standards and provides a brief overview of the problems and developmental characteristics of food safety in China. The composition and characteristics of China's food safety standards are revealed by an analysis of the changes in China's general food standards, an overview of the characteristics of the hygiene requirements in the production and operation process, and an introduction to food product and test method standards. In conclusion, Chinese food safety standards are still being improved, but they must also be effectively implemented and followed up in real time in order to continuously improve the quality of food and reduce food safety incidents. © 2024 Society of Chemical Industry.