bluuebunny/crossref_metadata_2025_split huggingface dataset:
A pan‐cancer analysis of prognostic significance and immunological role of lysosomal‐associated membrane protein 3
Authors: Feng Xuefei , Gao Lvye , Shen Xinyuan , Li Mingtai , Wang Xiaohui , Hao Yanlong , Chen Jinyan , Zhai Yuanfang , Zou Binbin , Yao Shangman , Guo Yanlin , Zhang Ling 
Published: December 2023

AbstractLysosomal dysfunction can drive carcinogenesis. Lysosomal‐associated membrane protein 3 (LAMP3), is a member of the Lysosome Associated Membrane Proteins and is involved in the malignant phenotype such as tumour metastasis and drug resistance, while the mechanisms that regulate the malignant progression of tumour remain vague. Our study aims to provide a more systematic and comprehensive understanding of the role of LAMP3 in the progression of various cancers by various databases.We explored the role of LAMP3 in pan‐cancer using The Cancer Genome Atlas (TCGA) and Genotype‐Tissue Expression (GTEx) database. Multiple online web platforms and software were used for data analysis, including HPA, TIMER, TISIDB, GEPIA, UALCAN, Kaplan–Meier plotter, DAVID and TIGER. The immunohistochemistry was used to quantify the LAMP3 and PD‐L1 expression levels in cancer.High LAMP3 expression was found in most cancers and differentially expressed across molecular and immune subtypes. The expression of LAMP3 was involved in the immune‐associated processes of Antigen processing and presentation, Th17 cell differentiation, Th1 and Th2 cell differentiation, and the immune‐associated pathways of T cell receptor and B cell receptor signalling pathways in most cancers. It also correlated with genetic markers of immunomodulators in various cancers. LAMP3 and PD‐L1 expression in BRCA and HNSC tissues was higher than that in corresponding adjacent normal tissues by immunohistochemistry. There is a significant correlation between the expression of LAMP3 and PD‐L1.Our study elucidates that LAMP3 has different expression patterns and genetic alteration patterns in different tumours. It is a potential biomarker for immune‐related cancer diagnosis, prognosis and efficacy prediction.
Gastrodin alleviates premature senescence of vascular endothelial cells by enhancing the Nrf2/HO‐1 signalling pathway
Authors: Tong Pengfei , Tian Ke , Bi Jiajia , Wang Ruihua , Wang Zhengfeng 
Published: December 2023

AbstractEndothelial dysfunction is an independent risk factor for stroke. The dysfunction of endothelial cells (EC) is closely concerned with EC senescence. Gastrodin (GAS) is an organic compound extracted from the dried root mass of the Orchidaceae plant Gastrodiae gastrodiae. It is used clinically to treat diseases such as vertebrobasilar insufficiency, vestibular neuronitis and vertigo. In the present study, we used hydrogen peroxide (H2O2)‐induced human umbilical vein endothelial cells (HUVECs) to establish an in vitro EC senescence model and to investigate the role and mechanism of GAS in EC senescence. It's found that H2O2‐treated HUVECs increased the proportion of senescence‐associated β‐galactosidase (SA β‐gal) positive cells and the relative protein expression levels of senescence‐associated cyclin p16 and p21. In addition, GAS reduced the proportion of SA β‐gal positive cells and the relative protein expression levels of p16 and p21, and increased the proliferation and migration ability of HUVECs. Meanwhile, GAS increased the expression of the anti‐oxidative stress protein HO‐1 and its nuclear expression level of Nrf2. The anti‐senescence effect of GAS was blocked when HO‐1 expression was inhibited by SnPPIX. Furthermore, absence of HO‐1 abolished the effect of GAS on HUVEC proliferation and migration. In conclusion, GAS ameliorated H2O2‐induced cellular senescence and enhanced cell proliferation and migration by enhancing Nrf2/HO‐1 signalling in HUVECs. These findings of our study expanded the understanding of GAS pharmacology and suggested that GAS may offer a potential therapeutic agent for stroke.
Drug survival, effectiveness and safety of ixekizumab for moderate‐to‐severe psoriasis up to 5 years
Authors: Mastorino L. , Dapavo P. , Burzi L. , Rosset F. , Giunipero di Corteranzo I. , Leo F. , Verrone A. , Stroppiana E. , Ortoncelli M. , Ribero S. , Quaglino P. 
Published: December 2023

AbstractIntroductionIxekizumab proved to be effective and safe for psoriasis treatment in several randomized clinical trials and real‐life studies. Nevertheless, long‐term real‐world experiences are still lacking, with little data up to 4 years of treatment.ObjectivesTo analyse survival, effectiveness and safety of ixekizumab in a real‐life cohort of patients affected by moderate‐to‐severe psoriasis or psoriatic arthritis up to 260 weeks (5 years).MethodsWe included all patients treated with ixekizumab from December 2017 to March 2021. Drug survival (DS) was analysed in patients at risk for up to 5 years. Cox analysis was adopted to evaluate possible predictive factors of discontinuation. Psoriasis Area Severity Index (meanPASI and PASI100, 90, and ≤3) was used as outcomes of effectiveness on observed patients at 16, 52, 104, 156, 208 and 260 weeks. Logistic regression was performed to identify possible predictive factors of response.ResultsDS was 65.5% at 260 weeks, with being a super‐responder patient (achievement of PASI100 at 16 weeks and maintained at 28 weeks) correlated with less risk of discontinuation. PASI100, 90 and ≤3 was achieved by 54.1%, 60.5% and 73% of observed patients, respectively, at 16 weeks, and by 59.1%, 81.8% and 95.5%, respectively, at 260 weeks. High mean BMI was the only factor strongly associated with less achievement of the outcomes at the earlier time points: PASI100 at 16 weeks (OR 0.93, CI 0.87–0.98, p = 0.014) and at 104 weeks (OR 0.91, CI 0.84–0.98, p = 0.019), PASI90 achievement at 16 weeks (OR 0.94, CI 0.88–0.99, p = 0.028) and 104 weeks (OR 0.91, CI 0.83–0.99, p = 0.027), and PASI ≤3 (OR 0.86, CI 0.76–0.97, p = 0.018) at 104 weeks. No severe adverse events were observed.ConclusionsIxekizumab showed high effectiveness and safety for up to 5 years, with survival of 2/3 of treated patients. Rapid response to treatment is predictive of long‐term response.
Increased occurrence of the rare golden color morph of Pacific chub Kyphosus sandwicensis in a no‐take marine reserve
Authors: Franklin Erik C. , Platt Madeleine T. , Andrade Pelika 
Published: December 2023

AbstractPacific chub, Kyphosus sandwicensis, are typically gray but some individuals display a golden color morph. We estimated that the frequency of occurrence of the golden morphs increased significantly from 2007 (1.9%) and 2012 (2.6%) to 2017 (5.0%) inside a no‐take marine reserve around Nihoa Island in the Northwestern Hawaiian Islands. While sporadic observations of a golden color morph have been noted for several other marine fish species, we provide a quantitative estimate of changes in the frequency of occurrence of the morph within a wild marine population.
Serum CXCL16: A new predictor of liver inflammation in patients with chronic hepatitis B
Authors: Wan Yawen , Mao Minxin , Li Ming , Liu Jiacheng , Tong Xin , Wang Jian , Li Jie , Yin Shengxia , Wu Chao 
Published: December 2023

AbstractThe prompt initiation of antiviral therapy is essential in patients with chronic hepatitis B (CHB), especially when severe liver inflammation is detected. However, transcutaneous liver puncture, the gold standard for assessing liver inflammation, is invasive and its widespread application is limited. Therefore, there is an urgent need for more non‐invasive markers to predict liver inflammation. In our retrospective cross‐sectional study, which included 120 CHB patients and 31 healthy subjects, we observed a significant increase in serum chemokine C‐X‐C‐motif ligand 16 (CXCL16) in CHB patients compared to healthy controls (p < .001). Notably, patients with severe inflammation (Scheuer's grade G ≥ 3, n = 26) exhibited a substantial increase in serum CXCL16 compared to those with non‐severe inflammation (Scheuer's grade G < 3, n = 96) [(median, IQR), 0.42 (0.24–0.71) ng/mL vs. 1.01 (0.25–2.09) ng/mL, p < .001]. Furthermore, we developed a predictive model that combined CXCL16 with platelet count (PLT), alanine aminotransferase (ALT) and albumin (ALB) to accurately predict liver inflammation in CHB patients. This model was more effective than ALT alone in predicting liver inflammation (AUC, 0.92 vs. 0.81, p = .015). Additionally, using an HBV‐transduced mouse model, we demonstrated that blocking CXCL16 led to a reduction in liver inflammation and impaired infiltration and function of natural killer T (NKT) and natural killer (NK) cells. These findings suggest that CXCL16 is a promising non‐invasive biomarker of liver inflammation in CHB patients and may play a role in inducing liver inflammation via a NKT and NK cell pathway.
Design and development of robotic collaborative system for automated construction of reciprocal frame structures
Authors: Chea Cheav Por , Bai Yu , Zhou Zhuomin 
Published: December 2023

AbstractRobotic technologies have shown their potential to improve efficiency, precision, and safety for construction tasks. In this paper, the concept of design for robotic construction (DfRC) is introduced, and robotic collaborative systems are developed for the construction of load‐carrying structures. An automated structural assembly was achieved and demonstrated through robotics with a preference for reciprocal frame (RF) structures. Key innovations include the use of magnetic temporary connections to minimize offsets of mobile robots and linear actuators as temporary supports during construction. Furthermore, specific procedures are formulated to navigate the robots and to target and install the components using a fiducial marker system and simultaneous localization and mapping packages, with consideration of structural deformation during construction due to self‐weight. The successful assembly of a 4.5‐m span RF structure demonstrates the potential of DfRC and the proposed robotic collaborative system in the automated construction of load‐carrying structures.
Germ‐soma differentiation and reproduction in a new species of early Cambrian acritarch
Authors: Liu Wei , Yin Zongjun , Pan Bing , Shen Bing , Dong Lin , Li Guoxiang 
Published: December 2023

AbstractAs a significant evolutionary innovation, multicellularity has independently evolved multiple times throughout the evolutionary history of eukaryotes, making a substantial contribution to their diversity. In retracing the multicellularity of eukaryotes, deep‐time fossil records play an irreplaceable role. In this paper, we report a new acritarch Concavaesphaera ornata gen. et sp. nov. from the early Cambrian Kuanchuanpu biota (535 Ma). These fossils are generally spherical, with diameters ranging from 450 to 950 μm, and feature an envelope with complex ornament. Inside, there are two groups of cells varying in size. Larger cells are relatively fewer in number and each resides within a sac‐like cavity distributed along the inner wall of the envelope. Statistical data show a positive correlation between the diameter and cavity volume during the development of large cells. Small cells are numerous, have a diameter of about 15 μm, and fill the entire interior of the specimens. We interpret the large cells as reproductive cells and the small cells as somatic cells. These observations show that Concavaesphaera evolved multicellular complexity equivalent to that of modern Volvox, with germ‐soma differentiation and separation. Available evidence suggests that the similarities between Concavaesphaera and Volvox are more likely to be the result of convergent evolution. Therefore, Concavaesphaera may represent an extinct multicellular eukaryote which evolved during the Cambrian explosion and probably documented an independent innovation of multicellularity in eukaryotic evolutionary history. This implies that multicellularity evolved many more times in eukaryotes than previously estimated.
Characteristics of an ethnically diverse cohort of children with pediatric cutaneous mastocytosis: One institution's experience
Authors: Capellan Vasquez Evelyn , Cosper Arjola , Ferastraoaru Denisa 
Published: December 2023

AbstractThere has been limited research exploring how the demographic characteristics of children with pediatric cutaneous mastocytosis (PCM) may influence both the cutaneous and systemic symptoms. In this observational retrospective study of 51 children with PCM, we found a significantly higher rate of gastrointestinal (GI) symptoms in children of Hispanic ethnicity (4/21,19%) compared to non‐Hispanics (0/30, 0%, p = 0.024). While this finding may reflect the high proportion of Hispanics in our population, a racial predisposition toward distinct systemic symptoms may be possible. We also found a significantly lower proportion of Hispanic children being diagnosed with PCM under the age of 3 years (47.6%) when compared with non‐Hispanic children (76.7%, p = 0.03), suggesting that more data are needed to further assess the role of ethnicity and healthcare disparities in PCM diagnosis. Larger prospective studies are necessary to better evaluate the association between ethnicity, early diagnosis, and systemic symptoms in PCM and to describe its impact on long‐term outcomes.
Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement
Authors: Moran Megan N. , Jones D. B. , Jensen S. A. , Marcoli R. , Jerry D. R. 
Published: December 2023

AbstractAquaculture is one of the fastest‐growing food production sectors. As the global human population continues to increase and further pressure is added to the prospects of achieving global food security, aquaculture is expected to play an integral role in meeting future nutrition demands. With advances in genetic technologies over recent years, much progress has been made within the realm of selective breeding. Despite success, selective breeding programs have limitations to the rate of genetic gain they can achieve. The incorporation of targeted genetic technologies, such as gene editing, into research related to selective breeding programs will help identify specific genes related to commercially desirable traits, as well as expedite genetic improvement. This review summarises research encompassing the most commonly targeted traits using gene editing within aquaculture, namely reproduction and development, pigmentation, growth and disease resistance. In addition, this review illustrates how the incorporation of gene editing can expedite genetic improvement through the rapid fixation of desirable alleles, as well as suggests strategies to accelerate genetic improvement for aquaculture production.
Determination of expression patterns of miR‐26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cows
Authors: Olğaç Kemal Tuna , Yazlik Murat Onur , Özkan Hüseyin , Kaya Ufuk , Tirpan Mehmet Borga 
Published: December 2023

AbstractFor maximum productivity in a dairy farm, the earliest and the most accurate detection of pregnancy is essential. The aim of this study was to determine the efficacy of expression patterns of miR‐26a, and serum Preimplantation Factor (PIF) levels for pregnancy diagnosis during the early pregnancy in nulliparous and multiparous cows. A total of 60 cows (30 nulliparous and 30 multiparous Holstein cows) were enrolled in the study. Blood samples were collected for miR‐26a on days 8 and 16 (D8 and D16), and for the PIF on days 10 and 20 (D10 and D20) following insemination (D0). Pregnancies were determined by ultrasonography on the 28th day after insemination. Expression levels of miR‐26a determined by qPCR. PIF levels were assessed by using commercial ELISA kits. All data were analyzed by using the MIXED procedure of SPSS. The expression levels of miR‐26a were 6.64 folds higher on D16 in pregnant compared to non‐pregnant multiparous cows (p < .05). On D8 and D16, miR‐26a expression levels were found higher 13 folds in pregnant compared to non‐pregnant nulliparous cows (p < .05). Additionally, miR‐26a expressions were higher 5.42 folds (p < .05) on D8, 7.19 folds higher (p < .01) on D16 in pregnant nulliparous and multiparous cows, and were 6.30 folds higher (p < .001) on D8 and D16 according to non‐pregnant animals. PIF levels were greater in pregnant animals (p < .05). Analyzing miR‐26a on D8 might be considered as sufficient in nulliparous cows. Pregnancy detection in multiparous cows can be made on the 16th day with this method. Furthermore, PIF evaluations may be sufficient on D10 in multiparous cows. Besides, PIF levels and miR‐26a expression levels might be used safely in field conditions and clinical applications.
The psychometric properties of the Burnout Assessment Tool in Norway: A thorough investigation into construct‐relevant multidimensionality
Authors: De Beer Leon T. , Christensen Marit , Sørengaard Torhild A. , Innstrand Siw T. , Schaufeli Wilmar B. 
Published: December 2023

ObjectiveThe World Health Organization recognizes burnout as an occupational issue. Nevertheless, accurately identifying employee burnout remains a challenging task. To complicate matters, current measures of burnout have demonstrated limitations, prompting the development of the Burnout Assessment Tool (BAT). Given these circumstances, conducting an in‐depth examination of the BAT's construct‐relevant multidimensionality is crucial.MethodThis study focuses on both the original 23‐item BAT and the short 12‐item version, using modern factor analytic methods to investigate reliability, validity, and measurement invariance in a representative sample from Norway (n = 493; 49.54% women).ResultsOur findings revealed that the bifactor exploratory structural equation modeling solution (burnout global factor and four specific burnout component factors) best explained the data for both BAT versions. All factors demonstrated adequate omega coefficients, with the global factor showing exceptional strength. Both BAT versions correlated highly with each other and with another burnout measure, suggesting convergent validity. Furthermore, both BAT versions achieved full (strict) measurement invariance based on gender. Finally, our results showed that burnout acts as a mediator in our proposed job demands–resources model as preliminary evidence of predictive validity.ConclusionsThe study validates the Burnout Assessment Tool in the Norwegian context. The study supports the reliability, validity, and unbiased nature of the tool across genders. The findings also reinforce the importance of job demands and resources, along with burnout as a key mediator, in understanding workplace dynamics in accordance with job demands–resources theory.
Consistent covariances estimation for stratum imbalances under minimization method for covariate‐adaptive randomization
Authors: Zhao Zixuan , Song Yanglei , Jiang Wenyu , Tu Dongsheng 
Published: December 2023

AbstractPocock and Simon's minimization method is a popular approach for covariate‐adaptive randomization in clinical trials. Valid statistical inference with data collected under the minimization method requires the knowledge of the limiting covariance matrix of within‐stratum imbalances, whose existence is only recently established. In this work, we propose a bootstrap‐based estimator for this limit and establish its consistency, in particular, by Le Cam's third lemma. As an application, we consider in simulation studies adjustments to existing robust tests for treatment effects with survival data by the proposed estimator. It shows that the adjusted tests achieve a size close to the nominal level, and unlike other designs, the robust tests without adjustment may have an asymptotic size inflation issue under the minimization method.
Informational rents and the excessive entry theorem: The case of hidden action*
Authors: de Pinto Marco , Goerke Laszlo , Palermo Alberto 
Published: December 2023

AbstractEntry in a homogeneous Cournot‐oligopoly is excessive if there is business‐stealing. These findings assume that production costs reduce profits and welfare equally. If firms pay informational rents due to frictions in the employer–employee relationship, production costs partly reflect transfers, which do not alter welfare directly. We investigate the excessive entry theorem in the presence of rents. We find that informational rents can invalidate the theorem. Rents reduce profits and deter entry into the market equilibrium, while the socially optimal number of firms is not affected directly. The rent effect becomes stronger the lower the number of firms and can overcompensate the business‐stealing externality. As an example, we model a hidden action problem in which employees have an informational advantage after signing the contract with the firm. Insufficient entry occurs if entry costs are sufficiently high because they lower the number of firms and raise informational rents.
Using synthetic control method to estimate the growth effects of economic liberalisation: Evidence from transition economies
Authors: Kantorowicz Jaroslaw , Spruk Rok 
Published: December 2023

AbstractWe examine the contribution of institutional reforms to economic growth. To this end, we distinguish between several classes of institutional reform in the approach to economic liberalisation. Based on a sample of 24 current and former transition economies for the period 1980–2016, we estimate the counterfactual scenarios related to each distinctive institutional approach to the economic liberalisation by making use of the synthetic control method. Our evidence uncovers notable contrasts in the long‐term effectiveness of designated policy approaches in recovering from the transitional recession. A variety of synthetic control estimates suggests that a sustained big bang approach (rapid reforms) appears to be the most effective approach, while an abortive and gradualist approach tends to produce a permanent breakdown of the growth trajectories relative to the estimated counterfactuals. The point estimates survive an extensive battery of placebo tests. By employing the same methodological tool kit, future research could examine the impact of transition reforms on other socio‐economic variables, such as the poverty rates, income inequalities, health and environmental outcomes.
The effect of obtaining EU citizenship in former transition economies on remittance flows
Authors: Pieroni Luca , Rossello Roig Melcior 
Published: December 2023

AbstractWe estimate the effect of the granting of European Union (EU) citizenship rights on the flow of remittances from Italy. We use the EU enlargements of 2007 and 2013 as natural experiments. The results show a negative impact on remittance flows. The effect of the 2007 EU enlargement shows how Romanian and Bulgarian citizens reduced the average annual amount of remittances by 1400 Euros per year per migrant. In the case of the 2013 EU enlargement, we find a smaller decrease of about 50 Euros per year per Croat. These results are consistent after a series of robustness checks. We analyse this decline in depth to the extent that remittances are an important source of monetary flows in transition economies. We find that both the change in the taxonomy of immigrants from the new Member States and the improvement of economic conditions in the country of origin after the EU accession provide suggestive evidence on plausible mechanisms behind our results.