bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Dual‐domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration
Authors: Jiang Yichao , Chen Xiaoqing , Ye Xinya , Wen Can , Xu Tao , Yu Chao , Ning Wenjing , Wang Guosong , Xiang Xinchu , Liu Xiaomin , Wang Yalin , Chen Yuanzhi , Liu Xue , Shi Changrong , Liu Chao , Yuan Quan , Chen Yixin , Zhang Tianying , Luo Wenxin , Xia Ningshao 
Published: February 2024

AbstractChronic hepatitis B (CHB) remains a major public health concern because of the inefficiency of currently approved therapies in clearing the hepatitis B surface antigen (HBsAg). Antibody‐based regimens have demonstrated potency regarding virus neutralization and HBsAg clearance. However, high dosages or frequent dosing are required for virologic control. In this study, a dual‐domain‐engineered anti‐hepatitis B virus (HBV) therapeutic antibody 73‐DY is developed that exhibits significantly improved efficacy regarding both serum and intrahepatic viral clearance. In HBV‐tolerant mice, administration of a single dose of 73‐DY at 2 mg kg−1 is sufficient to reduce serum HBsAg by over 3 log10 IU mL−1 and suppress HBsAg to < 100 IU mL−1 for two weeks, demonstrating a dose‐lowering advantage of at least tenfold. Furthermore, 10 mg kg−1 of 73‐DY sustainably suppressed serum viral levels to undetectable levels for ≈ 2 weeks. Molecular analyses indicate that the improved efficacy exhibited by 73‐DY is attributable to the synergy between fragment antigen binding (Fab) and fragment crystallizable (Fc) engineering, which conferred sustained viral suppression and robust viral eradication, respectively. Long‐term immunotherapy with reverse chimeric 73‐DY facilitated the restoration of anti‐HBV immune responses. This study provides a foundation for the development of next‐generation antibody‐based CHB therapies.
mRNA Sonotransfection of Tumors with Polymeric Microbubbles: Co‐Formulation versus Co‐Administration
Authors: Chen Junlin , Wang Bi , Wang Yuchen , Radermacher Harald , Qi Jinwei , Momoh Jeffrey , Lammers Twan , Shi Yang , Rix Anne , Kiessling Fabian 
Published: February 2024

AbstractDespite its high potential, non‐viral gene therapy of cancer remains challenging due to inefficient nucleic acid delivery. Ultrasound (US) with microbubbles (MB) can open biological barriers and thus improve DNA and mRNA passage. Polymeric MB are an interesting alternative to clinically used lipid‐coated MB because of their high stability, narrow size distribution, and easy functionalization. However, besides choosing the ideal MB, it remains unclear whether nanocarrier‐encapsulated mRNA should be administered separately (co‐administration) or conjugated to MB (co‐formulation). Therefore, the impact of poly(n‐butyl cyanoacrylate) MB co‐administration with mRNA‐DOTAP/DOPE lipoplexes or their co‐formulation on the transfection of cancer cells in vitro and in vivo is analyzed. Sonotransfection improved mRNA delivery into 4T1 breast cancer cells in vitro with co‐administration being more efficient than co‐formulation. In vivo, the co‐administration sonotransfection approach also resulted in higher transfection efficiency and reached deeper into the tumor tissue. On the contrary, co‐formulation mainly promoted transfection of endothelial and perivascular cells. Furthermore, the co‐formulation approach is much more dependent on the US trigger, resulting in significantly lower off‐site transfection. Thus, the findings indicate that the choice of co‐administration or co‐formulation in sonotransfection should depend on the targeted cell population, tolerable off‐site transfection, and the therapeutic purpose.
Splicing Factor PQBP1 Curtails BAX Expression to Promote Ovarian Cancer Progression
Authors: Liu Xihan , Zhang Jiaojiao , Wang Zixiang , Yan Mingyao , Xu Meining , Li Gaoyuan , Shender Victoria , Wei Jian‐jun , Li Jianqiao , Shao Changshun , Zhang Shiqian , Kong Beihua , Song Kun , Liu Zhaojian 
Published: February 2024

AbstractSplicing factor polyglutamine binding protein‐1 (PQBP1) is abundantly expressed in the central nervous system during development, and mutations in the gene cause intellectual disability. However, the roles of PQBP1 in cancer progression remain largely unknown. Here, it is shown that PQBP1 overexpression promotes tumor progression and indicates worse prognosis in ovarian cancer. Integrative analysis of spyCLIP‐seq and RNA‐seq data reveals that PQBP1 preferentially binds to exon regions and modulates exon skipping. Mechanistically, it is shown that PQBP1 regulates the splicing of genes related to the apoptotic signaling pathway, including BAX. PQBP1 promotes BAX exon 2 skipping to generate a truncated isoform that undergoes degradation by nonsense‐mediated mRNA decay, thus making cancer cells resistant to apoptosis. In contrast, PQBP1 depletion or splice‐switching antisense oligonucleotides promote exon 2 inclusion and thus increase BAX expression, leading to inhibition of tumor growth. Together, the results demonstrate an oncogenic role of PQBP1 in ovarian cancer and suggest that targeting the aberrant splicing mediated by PQBP1 has therapeutic potential in cancer treatment.
RBMS1 Coordinates with the m6A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation
Authors: Sun Yu , Chen Dan , Sun Siwen , Ren Menglin , Zhou Liang , Chen Chaoqun , Zhao Jinyao , Wei Huanhuan , Zhao Qingzhi , Qi Yangfan , Zhang Jinrui , Zhang Ge , Liu Han , Yang Qingkai , Liu Quentin , Wang Yang , Zhang Wenjing 
Published: February 2024

AbstractMetastasis is the leading cause for the high mortality of lung cancer, however, effective anti‐metastatic drugs are still limited. Here it is reported that the RNA‐binding protein RBMS1 is positively associated with increased lymph node metastasis in non‐small cell lung cancer (NSCLC). Depletion of RBMS1 suppresses cancer cell migration and invasion in vitro and inhibits cancer cell metastasis in vivo. Mechanistically, RBMS1 interacts with YTHDF1 to promote the translation of S100P, thereby accelerating NSCLC cell metastasis. The RRM2 motif of RBMS1 and the YTH domain of YTHDF1 are required for the binding of RBMS1 and YTHDF1. RBMS1 ablation inhibits the translation of S100P and suppresses tumor metastasis. Targeting RBMS1 with NTP, a small molecular chemical inhibitor of RBMS1, attenuates tumor metastasis in a mouse lung metastasis model. Correlation studies in lung cancer patients further validate the clinical relevance of the findings. Collectively, the study provides insight into the molecular mechanism by which RBMS1 promotes NSCLC metastasis and offers a therapeutic strategy for metastatic NSCLC.
Electrocatalytic Co‐Upcycling of Nitrite and Ethylene Glycol over Cobalt–Copper Oxides
Authors: Niu Zhaodong , Fan Shiying , Li Xinyong , Chen Guohua 
Published: February 2024

AbstractElectrocatalytic nitrite (NO2−) reduction reaction (NO2−RR) for ammonia (NH3) synthesis is a promising alternative for NO2− resource utilization. Herein, a dual‐site copper‐cobalt oxide catalyst is reported for the efficient electrocatalytic reduction of NO2− to NH3, exhibiting NH3 Faradaic efficiency that remained above 95% (0.1 m NaNO2) over a wide potential window (−0.1 to −0.6 V vs reversible hydrogen electrode, vs RHE). More importantly, the high NH3 Faradaic efficiency maintains an over 85% (−0.1 to −0.3 V vs RHE) at a low concentration of NaNO2 (0.01 m). Theoretical calculations demonstrate that CuO serves the *NO2 to *NO and is subsequently converted to NH3 on Co3O4. Coupled anodic ethylene glycol (EG) oxidation reaction endows low cell voltage (ΔU = 480 mV, 10 mA cm−2) and energy consumption saving (>23%) in a two‐electrode system. This work provides a reference for a co‐upcycling electrolyzer for NO2− and EG.
Prompt Molten Na Infusion and Fluidic Transport by Virtue of Versatile Nano Canal Defects on Tin Oxide Sodiophilic Hosts
Authors: Zeng Yuzhuo , Bai Weijing , Zhu Jianhui , Xu Maowen , Jiang Jian 
Published: February 2024

AbstractIntrinsically weak metal affinity and sluggish sodium (Na) nucleation/molten fluidic transport of conventional hosts impede the fabrication of high‐capacity Na anodes. Mediating surface free energies (SFEs) of hosts and reinforcing their intermolecular attraction to viscous Na fluids can radically solve these problems. Herein, “canaled” tin oxide nanorod arrays grown on a 3D carbon cloth (CC) matrix exhibit distinct polar/nonpolar SFE components that markedly elevate the solid–liquid wettability for molten Na is reported. Such nanocanal defects render strong capillary forces for spontaneous molten Na imbibition and help to instantly activate Na─Sn alloying and Na nucleation reactions. Furthermore, sodiophilic Na15Sn4 interlayers evolved in former alloying procedures also aid in reducing Na nucleation energy barriers and guide the planar electro‐deposition/dispersion, alleviating the uncontrolled Na dendrite growth. The derived Na/Na15Sn4/CC anodes can thus keep stable after 2000 h of galvanostatic cycling at 1 mA cm−2 or high‐rate testing, without notable dendritic formation. The packed full cells also show superior rate capability and cyclability (capacity retention over 91.5% after all cycling at 1 C). This work provides a smart nano‐engineering way to trigger a fast infusion of molten metals into hosts and synergistically facilitate Na fluidic transport, which may push forward the scalable application of Na metal batteries.
Peanut (Arachis hypogaea L.) response to low‐rate applications of selected herbicides at vegetative and reproductive growth stages
Authors: Singh Navjot , Daramola Olumide S. , Iboyi Joseph E. , Devkota Pratap 
Published: February 2024

AbstractOff‐target drift of herbicides can seriously reduce peanut (Arachis hypogea L.) growth and yield and is of great concern to growers who will need to manage sensitive crops near new herbicide‐tolerant crops. Field experiments were conducted in 2021 and 2022 with 25% labeled rates of dicamba, glufosinate, glyphosate, lactofen, and paraquat to simulate drift on peanut. The objective was to evaluate the effects of low‐rate application of the herbicides on peanut injury and yield reductions and to determine if unmanned aerial vehicle (UAV) imagery‐based normalized difference vegetation index (NDVI) provides accurate estimation of peanut injury from the herbicides applied at vegetative (V3) and reproductive (R3) growth stages. Peanut suffered greater yield reduction (33%) when exposed to the herbicides at R3 than at V3 growth stage (19%) across all herbicides applied. The order of herbicides that induced yield reductions in peanut was glyphosate > glufosinate = dicamba > paraquat = lactofen. Regardless of exposure timing, NDVI values generated from UAV imagery could not differentiate paraquat or lactofen injury from the weed‐free check. However, NDVI values could differentiate between injured and weed‐free check plants up to 2 and 4 weeks after treatment (WAT) for dicamba at R3 and V3 exposure timing, respectively, up to 4 WAT for glufosinate, and 8 WAT for glyphosate. NDVI from aerial imagery may be helpful to accelerate the detection of injury in large hectarages with greater accuracy compared with visual injury rating, which can be influenced by individual estimation bias.
Work–rest regimens for work in hot environments: A scoping review
Authors: Deshayes Thomas A. , Hsouna Hsen , Braham Mounir A. A. , Arvisais Denis , Pageaux Benjamin , Ouellet Capucine , Jay Ollie , Maso Fabien D. , Begon Mickael , Saidi Alireza , Gendron Philippe , Gagnon Daniel 
Published: February 2024

AbstractBackgroundTo limit exposures to occupational heat stress, leading occupational health and safety organizations recommend work–rest regimens to prevent core temperature from exceeding 38°C or increasing by ≥1°C. This scoping review aims to map existing knowledge of the effects of work–rest regimens in hot environments and to propose recommendations for future research based on identified gaps.MethodsWe performed a search of 10 databases to retrieve studies focused on work–rest regimens under hot conditions.ResultsForty‐nine articles were included, of which 35 were experimental studies. Most studies were conducted in laboratory settings, in North America (71%), on healthy young adults, with 94% of the 642 participants being males. Most studies (66%) employed a protocol duration ≤240 min (222 ± 162 min, range: 37–660) and the time‐weighted average wet‐bulb globe temperature was 27 ± 4°C (range: 18–34). The work–rest regimens implemented were those proposed by the American Conference of Governmental and Industrial Hygiene (20%), National Institute of Occupational Safety and Health (11%), or the Australian Army (3%). The remaining studies (66%) did not mention how the work–rest regimens were derived. Most studies (89%) focused on physical tasks only. Most studies (94%) reported core temperature, whereas only 22% reported physical and/or mental performance outcomes, respectively. Of the 35 experimental studies included, 77% indicated that core temperature exceeded 38°C.ConclusionsAlthough work–rest regimens are widely used, few studies have investigated their physiological effectiveness. These studies were mainly short in duration, involved mostly healthy young males, and rarely considered the effect of work–rest regimens beyond heat strain during physical exertion.
Differential DNA methylation in the brain as potential mediator of the association between traffic‐related PM2.5 and neuropathology markers of Alzheimer's disease
Authors: Li Zhenjiang , Liang Donghai , Ebelt Stefanie , Gearing Marla , Kobor Michael S. , Konwar Chaini , Maclsaac Julie L. , Dever Kristy , Wingo Aliza P. , Levey Allan I. , Lah James J. , Wingo Thomas S. , Hüls Anke 
Published: February 2024

AbstractINTRODUCTIONGrowing evidence indicates that fine particulate matter (PM2.5) is a risk factor for Alzheimer's disease (AD), but the underlying mechanisms have been insufficiently investigated. We hypothesized differential DNA methylation (DNAm) in brain tissue as a potential mediator of this association.METHODSWe assessed genome‐wide DNAm (Illumina EPIC BeadChips) in prefrontal cortex tissue and three AD‐related neuropathological markers (Braak stage, CERAD, ABC score) for 159 donors, and estimated donors’ residential traffic‐related PM2.5 exposure 1, 3, and 5 years prior to death. We used a combination of the Meet‐in‐the‐Middle approach, high‐dimensional mediation analysis, and causal mediation analysis to identify potential mediating CpGs.RESULTSPM2.5 was significantly associated with differential DNAm at cg25433380 and cg10495669. Twenty‐four CpG sites were identified as mediators of the association between PM2.5 exposure and neuropathology markers, several located in genes related to neuroinflammation.DISCUSSIONOur findings suggest differential DNAm related to neuroinflammation mediates the association between traffic‐related PM2.5 and AD.Highlights
First study to evaluate the potential mediation effect of DNA methylation for the association between PM2.5 exposure and neuropathological changes of Alzheimer's disease.
Study was based on brain tissues rarely investigated in previous air pollution research.
Cg10495669, assigned to RBCK1 gene playing a role in inflammation, was associated consistently with 1‐year, 3‐year, and 5‐year traffic‐related PM2.5 exposures prior to death.
Meet‐in‐the‐middle approach and high‐dimensional mediation analysis were used simultaneously to increase the potential of identifying the differentially methylated CpGs.
Differential DNAm related to neuroinflammation was found to mediate the association between traffic‐related PM2.5 and Alzheimer's disease.
Conceptual range estimation for total cost of ownership of modular process‐intensified chemical plants
Authors: Alhamouri Khaled I. , O'Connor James T. , Haapala Karl R. , Paul Brian K. 
Published: February 2024

AbstractChemical companies have used modularization to reduce capital costs and project timelines, putting capital to work faster and lowering the risk of entering new markets. Nevertheless, the impacts of using modularization along with advanced technologies, such as process intensification, have not yet been fully realized, often due to the uncertain business risks associated with their implementation. Therefore, new methods are needed for quantifying the impact of modular chemical process intensification (MCPI) on the capital and operating costs of chemical plants to help build a business case for this novel approach. This article presents a new conceptual range estimation technique for total cost of ownership that addresses the deficiencies of existing methods for quantifying MCPI impacts. The incorporation of percentage range estimates was employed to allow for adaptability across various cost and size scales. This work also begins to elucidate how chemical engineering and construction firms can benefit from MCPI and identifies barriers that inhibit MCPI applications in the chemical industry.
Transcript Profiles of Microglia/Macrophage Cells at the Borders of Chronic Active and Subpial Gray Matter Lesions in Multiple Sclerosis
Authors: Chomyk Anthony , Kucinski Rikki , Kim Jihye , Christie Emilie , Cyncynatus Kaitlyn , Gossman Zachary , Chen Zhihong , Richardson Brian , Cameron Mark , Turner Tim , Dutta Ranjan , Trapp Bruce 
Published: February 2024

ObjectiveMicroglia/macrophages line the border of demyelinated lesions in both cerebral white matter and the cortex in the brains of multiple sclerosis patients. Microglia/macrophages associated with chronic white matter lesions are thought to be responsible for slow lesion expansion and disability progression in progressive multiple sclerosis, whereas those lining gray matter lesions are less studied. Profiling these microglia/macrophages could help to focus therapies on genes or pathways specific to lesion expansion and disease progression.MethodsWe compared the morphology and transcript profiles of microglia/macrophages associated with borders of white matter (WM line) and subpial gray matter lesions (GM line) using laser capture microscopy. We performed RNA sequencing on isolated cells followed by immunocytochemistry to determine the distribution of translational products of transcripts increased in WM line microglia.ResultsCells in the WM line appear activated, with shorter processes and larger cell bodies, whereas those in the GM line appear more homeostatic, with smaller cell bodies and multiple thin processes. Transcript profiling revealed 176 genes in WM lines and 111 genes in GM lines as differentially expressed. Transcripts associated with immune activation and iron homeostasis were increased in WM line microglia, whereas genes belonging to the canonical Wnt signaling pathway were increased in GM line microglia.InterpretationWe propose that the mechanisms of demyelination and dynamics of lesion expansion are responsible for differential transcript expression in WM lines and GM lines, and posit that increased expression of the Fc epsilon receptor, spleen tyrosine kinase, and Bruton's tyrosine kinase, play a key role in regulating microglia/macrophage function at the border of chronic active white matter lesions. ANN NEUROL 2024;95:907–916
Thiophene π‐Bridge Manipulation of NIR‐II AIEgens for Multimodal Tumor Phototheranostics
Authors: Gui Yixiong , Wang Yuanwei , Wang Deliang , Qin Yi , Song Guangjie , Yan Dingyuan , Tang Ben Zhong , Wang Dong 
Published: February 2024

AbstractThe fabrication of a multimodal phototheranostic platform on the basis of single‐component theranostic agent to afford both imaging and therapy simultaneously, is attractive yet full of challenges. The emergence of aggregation‐induced emission luminogens (AIEgens), particularly those emit fluorescence in the second near‐infrared window (NIR‐II), provides a powerful tool for cancer treatment by virtue of adjustable pathway for radiative/non‐radiative energy consumption, deeper penetration depth and aggregation‐enhanced theranostic performance. Although bulky thiophene π‐bridges such as ortho‐alkylated thiophene, 3,4‐ethoxylene dioxythiophene and benzo[c]thiophene are commonly adopted to construct NIR‐II AIEgens, the subtle differentiation on their theranostic behaviours has yet to be comprehensively investigated. In this work, systematical investigations discovered that AIEgen BT‐NS bearing benzo[c]thiophene possesses acceptable NIR‐II fluorescence emission intensity, efficient reactive oxygen species generation, and high photothermal conversion efficiency. Eventually, by using of BT‐NS nanoparticles, unprecedented performance on NIR‐II fluorescence/photoacoustic/photothermal imaging‐guided synergistic photodynamic/photothermal elimination of tumors was demonstrated. This study thus offers useful insights into developing versatile phototheranostic systems for clinical trials.
Co‐research interview—collaborative way to learn from experience
Authors: Rautiainen Eija‐Liisa , Rautkallio Aino Maija 
Published: February 2024

AbstractCo‐research interview developed by Tom Andersen and colleagues is a collaborative way of evaluating therapeutic processes that value the experiences of both clients and professionals. This article describes our experiences and thoughts on this interview method. We present the interview structure and give suggestions on how to conduct the interview. We also present applications of the interview method and engage in a dialogue among ourselves about our experiences with these interviews.
Aromatic amino acids as latent, bio‐based curing agents and toughening agents for epoxy resins
Authors: Rothenhäusler Florian , Ruckdäschel Holger 
Published: February 2024

AbstractIn the realm of bio‐based curing agents, recent investigations have focused on amino acids owing to their distinctive attributes. Nevertheless, the suitability of thermosets cured with aromatic amino acids as latent matrix materials for fiber‐reinforced composites remains to be empirically established. Consequently, this study is oriented toward assessing the mechanical properties of diglycidyl ether of bisphenol A when cured with either L‐tryptophan or L‐tyrosine, in the presence of a latent, urea‐based accelerator. The investigated properties include glass transition temperatures, tensile, flexural, compression, and fracture toughness properties. The predominant variations in the mechanical characteristics of these thermosets are confined to their Young's moduli and fracture toughness properties. This divergence is attributed to the greater presence of crystals in the L‐tyrosine‐cured thermoset, resulting in enhanced reinforcement and toughening effects compared to the L‐tryptophan‐cured thermoset.