bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of ultrasonic composite pulsating heat pipe reinforced phase change material heat storage research
Authors: Han Xiaochun , Luo Xiaoxue , Zhang Xiaojun , Cao Shibo , Hao Huili 
Published: October 2023

Abstract
Studies have shown that ultrasonic waves can enhance heat transfer in Pulsating Heat Pipes, it also enhances the heat storage of Phase Change Materials, however, there are few studies on ultrasonic composite Pulsating Heat Pipes reinforced phase change material heat storage. In this paper, a phase change material heat storage device under ultrasonic field is designed, and the enhanced heat transfer performance of ultrasonic composite Pulsating Heat Pipes is studied and analyzed. Experimentally observed the change of heat storage efficiency of the heat storage device caused by ultrasonic wave at different power and different positions. The results show that : under the same ultrasonic (28KHz)frequency, after applying power of 50W、100W and 120W ultrasonic fields on one side of the heat storage device, the heat storage efficiency increased by 55.6%、 57.5% and 67.1% respectively ; the heat transfer coefficients under power of 50W and 120W ultrasonic fields are respectively Increased by 6.1% and 12.6% ; after applying 50W、100W and 120W ultrasonic fields on both side of the heat storage device, the heat storage efficiency increased by 44.5% 、 60.4% and 73.4% respectively, and the heat storage efficiency increased non-linearly. Then the heat storage process was analyzed, and it was found that the heat transfer coefficient fluctuated, and the heat transfer coefficient reached the maximum when the phase transition temperature was just reached. In addition, it was found that ultrasonic waves combined with Pulsating Heat Pipes enhanced heat transfer, which significantly shortened the start-up time of pulsating heat pipes, and verified the enhanced heat transfer of supercooled boiling of Pulsating Heat Pipes under ultrasonic fields; the research results can be used to design closed phase change thermal energy storage units, would provide useful inspiration for further development and application of Pulsating Heat Pipes and Phase Change Materials with ultrasonic.
Mood swings and risk for cancer: A Mendelian randomization analysis
Authors: Zhang Wangjin , Chen Jinghui , Zhang Tingting , Qiu Sihua , huang yingdan 
Published: October 2023

Abstract
Background: Despite significant advances in the treatment and prevention of cancer, it remains a leading cause of death globally, imposing a substantial economic burden. In this study, we employed Mendelian randomized studies to explore the causal relationship between mood swings and the risk of cancer.
Methods: We conducted a large-scale genome-wide association study (GWAS) to extract instrumental variables (IVs) that were strongly associated with exposure factors through genetic variation. We used three methods, namely inverse variance weighting (IVW), Weighted median, and MR Egger, to analyze the data and obtain preliminary results. We further used sensitivity analysis to verify the reliability of our findings. We used Cochran's Q value to detect heterogeneity, the MR-Egger intercept test to detect polymorphism and a leave-one-out analysis was conducted to evaluate the impact of removing a single SNP on the results.
Results: Our findings that mood swings are associated with an increased risk of endometrial cancer and ER- breast cancer. Our MR analysis indicates that mood swings are not associated with the risk of ER+ breast cancer, lung cancer, colorectal cancer, bladder cancer, prostate cancer, and ovarian cancer.
Conclusion: Our MR study reveals that mood swings increase the risk of endometrial cancer and ER-breast cancer.
Tetrahydroxy stilbene glycoside protects N2a/APP695swe cells by regulating Mfn2
Authors: Wang Yanyun , Ye Qunying , Qian Jun , Liu Zhipeng , Luo Hongbo 
Published: October 2023

Abstract
Background:
To investigate the effect of tetrahydroxy stilbene glycoside (TSG) on mitochondrial function and apoptosis of N2a/APP695swe cells by regulating the expression level of mitofusin 2 (Mfn2) and to further explore the mechanism of the effect of TSG on cell protection.
Methods:
The CCK8 method was used to analyze the cell viability of N2a/APP695swe cells treated with different concentrations of TSG for 48 h and to select the appropriate concentration of TSG for subsequent experiments. N2a/WT cells and N2a/APP695swe cells were routinely cultured in vitro, and the experimental cells were divided into four groups: the blank control group (WT group): N2a/WT cells, routinely cultured; the model group (APP group): N2a/APP695swe cells, routinely cultured; the treatment group (TSG group): N2a/APP695swe cells, appropriate concentrations of TSG intervention; and the inhibition group (siRNA group): N2a/APP695swe cells, siRNA used to inhibit Mfn2 expression, appropriate concentrations of TSG intervention. The JC-1 method was used to detect mitochondrial membrane potential, the TUNEL method was used to observe apoptosis by fluorescence microscopy, WB was used to detect protein expression of Mfn2, Bcl-2, Bax, Caspase3, Caspase9, and Cytochrome C, and RT‒qPCR was used to detect Mfn2, Bcl-2, Bax, Caspase3, and Caspase9 mRNA expression.
Results:
A concentration of 100 μmol/L TSG was selected for the experiment (P< 0.01). Compared with the WT group, the APP group showed decreased membrane potential (P<0.05), increased apoptosis (P<0.05), increased expression of cytochrome C, Bax, Caspase3, and Caspase9 (P<0.05), and decreased expression of Mfn2 and Bcl-2 (P <0.05). Compared with the APP group, the TSG group showed increased membrane potential (P<0.05), decreased apoptosis rate (P<0.05), decreased expression of Bax, Caspase3, Caspase9, and cytochrome C (P<0.05), and elevated expression of Mfn2 and Bcl-2 (P<0.05) in the siRNA group. Caspase9 and cytochrome C expression was decreased (P<0.05), Bcl-2 expression was elevated (P<0.05), and Mfn2 expression was decreased (P<0.05) in the siRNA group. Compared with the TSG group, the membrane potential level was decreased (P<0.05) and the apoptosis rate was increased (P<0.05) in the siRNA group, and the expression of Bax, Caspase3 and Caspase9, and cytochrome C was elevated (P<0.05) and the expression of Mfn2 and Bcl-2 was decreased (P<0.05) in the siRNA group.
Conclusion:
TSG has a clear protective effect in N2a/APP695swe cells, which may be associated with upregulating Mfn2, elevating mitochondrial membrane potential, increasing Bcl-2 expression and reducing the release of Bax, Caspase3, Caspase9, and cytochrome C, thereby ameliorating cellular mitochondrial dysfunction and inhibiting apoptosis.
Diversified Recommendation Algorithm Based on Penalty Factors
Authors: fan deng , Jiabin wang , Sheng Lv 
Published: October 2023

Abstract

Personalized recommendation system is a technology that uses user behavior and preference information to provide personalized recommendations for users. With the development of the Internet and the era of information explosion, personalized recommendation systems have been widely used in e-commerce, social media, music, video and other fields. Negative feedback-based user diversity recommendation algorithms aim to provide richer and diverse recommendation results to satisfy users' different interests and needs. Traditional recommender systems usually face the problems of over-personalization and user information bubbling because they mainly rely on positive feedback signals (user clicks, purchases, etc.), which may lead to users being recommended similar content and ignoring potential diversity. At the same time, due to the influence of social attributes, people tend to be influenced by mainstream elements, which leads to the problem of "information cocoon" when recommending, although it can still recommend content for the user's satisfaction, but does not really take into account the user's own interests and needs. This paper combines a series of commonly used recommendation algorithms, such as itemCF, userCF, CB, etc., to take into account the social type at the same time, more consideration of the user's own personalized recommendations, and the introduction of "negative feedback" mechanism to reduce the popular labels on the impression of the user's real interests, to further explore more innovative and interesting content, to achieve the effect of The effect of thousands of people is realized.
Сurrent state of Vairimorpha spp. infection cases and haplotype distribution across Eurasian countries
Authors: Shamaev Nikolai D. 
Published: October 2023

Abstract
Current state of Vairimorpha spp. infection cases in A. mellifera across Eurasia was characterized using electronic databases. Most of the study regions reported presence of mixed infection with Vairimorpha spp, excluding South-West and South-Central parts of Eurasia, where only V. ceranae was present. No reports were found in the North-East part of Eurasia. Additionally, the presence of Vairimorpha spp. Ptp3 amino acid local haplotypes in the Republic of Tatarstan, Russian Federation was examined. As a result, two local haplotypes of V. ceranae were found: the first one was identical to those from Netherlands and South Korea and the second one was a new haplotype, different from those isolated in Slovenia and Thailand. Two local haplotypes of V. apis were found in the local apiary of Republic of Tatarstan: first one was identical to those isolated in Slovenia, Spain and Turkey and the second one was a new haplotype, similar to above mentioned ones.
Revealing the expression characteristics and potential regulatory mechanisms of hub oxidative stress related genes of spinal cord injury
Authors: Li Jianfeng , Tong Kuileung , Zhou Jiaxiang , Li Shiming , He Zhongyuan , Wang Fuan , Chen Hongkun , Li Haizhen , Cheng Gang , Li Junhong , Zhou Zhiyu , Gao Manman 
Published: October 2023

Abstract
Spinal cord injury (SCI) induces oxidative stress reactions, exacerbating secondary damage. Moderating oxidative stress after SCI holds paramount significance for alleviating secondary injury. At the same time, comprehensive investigations into the pathogenesis of oxidative stress in SCI remain limited. In this study, we analyzed public datasets to identify differentially expressed oxidative stress-related genes (DEOSRGs) at various post-injury time points, identifying 25 hub oxidative stress-related genes (OSRGs). We also conducted tests and validations to ascertain the temporal expression patterns of some hub genes at both the tissue and single-cell levels. Subsequently, we unveiled the association between these hub genes and immune cell infiltration. Functional and pathway enrichment analyses were conducted on DEOSRGs at distinct time points, revealing alterations in enriched functions and signaling pathways. Additionally, we summarized potential communication signaling characteristics related to oxidative stress among different cells at various time points at the single-cell level, along with associated ligand-receptor pairs. As a subsequent step, we constructed mRNA-miRNA/mRNA-miRNA-lncRNA regulatory networks related to oxidative stress in SCI and analyzed transcription factors associated with hub OSRGs. Finally, leveraging the DSigDB database, we predicted compounds capable of inhibiting eight hub genes, offering potential drugs or molecules for targeted interventions in oxidative stress following SCI. Consequently, this study holds significance for gaining deeper insights into oxidative stress mechanisms after SCI and timely targeted interventions in oxidative stress following SCI.
Knowledge, Attitudes, and Practices of Primary Healthcare Practitioners in Low- and Middle- Income Countries: A Scoping Review on Genetics
Authors: Walters Sarah , Aldous Colleen , Malherbe Helen 
Published: October 2023

Abstract

Background:
The growing application of genetics in individualized treatments calls for an increased role of primary healthcare practitioners (pHCPs) in diagnosing and caring for individuals with genetic conditions. PHCPs' genetics knowledge and practices must be current to ensure adequate care.
Method:
A scoping review was conducted to explore peer-reviewed articles on the knowledge, attitudes, and practices of pHCPs concerning genetics, genetic testing, and genetic services. English-language articles published between January 1990 and April 2022 in low- and middle-income countries (LMICs) with an emphasis on human genetics/genomics were included.
Results:
Twenty-eight articles from 16 LMICs in five World Health Organization-defined regions met the inclusion criteria. Over the 32 years, the number of articles from LMICs increased steadily. The Eastern Mediterranean Region (EMR) contributed the most articles (n=8), while the Western Pacific Region (WPR) had the least (n=2). Among the countries, Brazil published the most articles (n=6), while ten countries contributed one article each. Fifteen articles had components of knowledge, 19 on attitudes towards genetics, and eight included genetic practices.
The results revealed a lack of knowledge among pHCPs concerning genetic diseases, emerging technologies, referral processes to genetic specialists, and clinical guidelines for managing genetic conditions. However, pHCPs attitudes towards genetic services and new technologies were generally positive. Identified barriers to genetic services included inadequate capacity, financial limitations, and religious beliefs.
Conclusions:
Addressing identified knowledge gaps through education is crucial for appropriate care for patients and families affected by genetic conditions. The limited published literature in LMICs on pHCPs' genetics knowledge, attitudes, and practices highlights the need for more targeted research on educational interventions. Improving pHCPs' ability to care for individuals and families with genetic conditions will ultimately lead to better patient outcomes and support for affected families.
Association between the Dietary Inflammatory Index and Hypertension in Middle-Aged and Elderly population in China: Results from a nation-wide cross sectional study
Authors: Dong Weihua , Qing-qing Man , Jian Zhang , Zhen Liu , Wei-yi Gong , Li-yun Zhao , Peng-kun SONG , Gang-qiang Ding 
Published: October 2023

Abstract
Background:
Evidence on the Association between Dietary Inflammatory Index and Risk of Hypertension among Chinese Population aged 45 and above
Objective：
To investigate the association between DII and hypertension in Chinese adults aged 45 years and above.
Methods：
Data was from the China Adults Chronic Diseases and Nutrition Surveillance (2015) for participants who were 45 years of age or above. The DII for each participant was determined through a combination of 3 day 24-hour dietary recall interviews and a food frequency questionnaire. Spatial analysis was employed to investigate the geographic distribution of DII in China. Furthermore, restricted cubic spline models and binary logistic regression analysis were used to assess the relationship between DII and hypertension.
Results：
A total of 39,282 middle-aged and elderly participants were included in the study, among whom 6,133 had hypertension. The DII scores of participants with hypertension were higher compared to those without hypertension (1.507 vs. 1.447, P = 0.003). There was a significant increasing trend in the proportion of hypertensive individuals as DII scores increase (P for trend = 0.004). Spatial analysis revealed a clear spatial correlation in the national distribution of DII scores (Moran I: 0.252, P = 0.001), with higher DII scores concentrated in the northwest region and lower DII scores concentrated in the southeast region. Restricted cubic spline models and binary logistic regression analysis demonstrated a positive association between DII and hypertension after adjusting for potential confounding factors.
Conclusions：
Dietary inflammatory index was positively associated with hypertension in Chinese adults aged 45 years and above, and dietary intervention might be a promising method in the prevention of hypertension.
Observation and Study on Material and Energy Exchange of Paddy at Different Growth Stages in the Liaohe Delta, Northeast China
Authors: Chao Yuan , Dongnan Li , Pengfei Sun , Qingyu Jia , Jing Miao , Qiong Wu , Kader MAVLAN 
Published: October 2023

Abstract
This study utilized observational data from the Panjin Wetland Ecosystem Field Observation Station during January to December 2019 to analyze water, heat, and carbon flux characteristics, as well as radiation balance, at different growth stages of paddy (transplanting, jointing, heading, and maturation) in Panjin. The results show that: (1) The jointing stage exhibited the highest average temperature, while the heading stage experienced the heaviest precipitation, approximately 3.3 times that of other periods. (2) Sensible heat flux followed the order of jointing stage > maturation stage > heading stage > transplanting stage, with negative values at night and positive values during the day. Latent heat flux followed the order of transplanting stage > heading stage > jointing stage > maturation stage, with positive latent heat flux throughout the day. Net ecosystem exchange (NEE) followed the order of transplanting stage > maturation stage > jointing stage > heading stage, with the transplanting stage acting as a carbon source and the heading stage exhibiting the strongest carbon fixation capacity. (3) Solar shortwave radiation dominated during different growth stages of paddy, with net radiation following the order of jointing stage > transplanting stage > heading stage > maturation stage. (4) Latent heat was the dominant energy exchange during different growth stages of paddy. The energy closure ratio was highest in the maturation stage, followed by the jointing stage, transplanting stage, and heading stage. The energy closure ratio in the maturation stage reached 69.63%, indicating good energy closure, while it was only 38.88% in the heading stage, indicating poor energy closure. (5) Sensible and latent heat fluxes generally exhibited positive correlations with environmental factors during different growth stages, while NEE showed a negative correlation. Photosynthetically active radiation and net radiation are identified as key factors influencing sensible heat flux, latent heat flux, and NEE. Evaporation has a significant impact on the latent heat flux. Additionally, air temperature, soil temperature, and vapor pressure deficit also play significant roles.
Identification of candidate chemosensory genes in Bactrocera cucurbitae based on antennal transcriptome analysis
Authors: wang jingjing , ma chao , Yue Yang , yang jingfang , chen lixiang , wang yiting , zhao chenchen , gao xuyuan , chen hongsong , ma weihua , zhou zhongshi 
Published: October 2023

Abstract
The melon fly, Bactrocera cucurbitae (Coquillett), is an invasive pest that poses a significant threat to agriculture in Africa and other regions. Flies are known to use their olfactory systems to recognise environmental chemical cues, however, the molecular components of the B. cucurbitae's chemosensory system are currently poorly characterised. To address this knowledge gap, we have used next-generation sequencing to analyse the antenna transcriptomes of sexually immature B. cucurbitae adults. The results identified 160 potential chemosensory genes, including 35 odourant-binding proteins (OBPs), 1 chemosensory protein (CSP), 3 sensory neuron membrane proteins (SNMPs), 70 odourant receptors (ORs), 30 ionotropic receptors (IRs), and 21 gustatory receptors (GRs). Quantitative real-time polymerase chain reaction (qPCR) was used to validate the results by assessing the expression profiles of 25 ORs and 15 OBPs. Notably, high expression levels for BcucOBP5/9/10/18/21/23/26 were observed in both the female and male antennae. Furthermore, BcucOROrco/6/7/9/13/15/25/27/28/42/62 exhibited biased expression in the male antennae, whereas BcucOR55 showed biased expression in the female antennae. This comprehensive investigation provides valuable insights into insect olfaction at the molecular level and will thus help to facilitate the development of enhanced pest management strategies in the future.
Metabolomic analysis in Amycolatopsis keratiniphila disrupted the competing ECO0501 pathway for enhancing the accumulation of vancomycin
Authors: Chen Shuo , Rao Min , Jin Wenxiang , Hu Mengyi , Chen Daijie , Ge Mei , Mao Wenwei , Qian Xiuping 
Published: October 2023

Abstract

Vancomycin is a clinically important glycopeptide antibiotic against Gram-positive pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). In the mutant strain of A.keratiniphila HCCB10007 Δeco-cds4-27, the production of ECO-0501 was disrupted, but enhanced vancomycin yield by 55% was observed compared with the original strain of A.keratiniphila HCCB10007. To gain insights into the mechanism about enhancement production of vancomycin in the mutant strain, comparative metabolomics analyses were performed between the mutant strain and the original strain A.keratiniphila HCCB1007 via GC-TOF-MS and UPLC-HRMS. The results of PCA and OPLS-DA revealed the significant distinction of the intracellular metabolites between the two strains during the fermentation process. 64 intracellular metabolites, which involved in amino acids, fatty acids and central carbon metabolism, were identified as differential metabolites. The high-yield mutant strain maintained high levels of glucose-1-phosphate and glucose-6-phosphate and they declined with the increases of vancomycin productions. Particularly, a strong association of fatty acids accumulation as well as 3,5-dihydroxyphenylacetic acid and non-proteinogenic amino acid 3,5-dihydroxyphenylglycine (Dpg) with enhancement of vancomycin production was observed in the high-yield mutant strain, indicating that the consumption of fatty acid pools might be benefit for giving rise to 3,5-dihydroxyphenylacetic acid and Dpg which further lead to improve vancomycin production. In addition, the lower levels of glyoxylic acid and lactic acid and higher levels of sulfur amino acids might be benefit for improving vancomycin production. These findings proposed more advanced elucidation of metabolomic characteristics in the high-yield strain for vancomycin production and could provide potential strategies to enhance the vancomycin production.