bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preclinical evidence for anaplastic lymphoma kinase inhibitors as novel therapeutic treatments for cholangiocarcinoma
Authors: Myint Kyaw Zwar , Sueca-Comes Mireia , Collier Pamela , Balasubramanian Brinda , Venkatraman Simran , Gordan John , Zaitoun Abed M. , Mukherjee Abhik , Arora Arvind , Larbcharoensub Noppadol , Suriyonplengsaeng Chinnawut , Wongprasert Kanokpan , Janvilisri Tavan , Gomez Dhanny , Grabowska Anna M. , Tohtong Rutaiwan , Bates David O. , Yacqub-Usman Kiren 
Published: December 2023

IntroductionBile duct cancer (cholangiocarcinoma, CCA) has a poor prognosis for patients, and despite recent advances in targeted therapies for other cancer types, it is still treated with standard chemotherapy. Anaplastic lymphoma kinase (ALK) has been shown to be a primary driver of disease progression in lung cancer, and ALK inhibitors are effective therapeutics in aberrant ALK-expressing tumors. Aberrant ALK expression has been documented in CCA, but the use of ALK inhibitors has not been investigated. Using CCA cell lines and close-to-patient primary cholangiocarcinoma cells, we investigated the potential for ALK inhibitors in CCA.MethodsALK, cMET, and ROS1 expression was determined in CCA patient tissue by immunohistochemistry and digital droplet polymerase chain reaction, and that in cell lines was determined by immunoblot and immunofluorescence. The effect on cell viability and mechanism of action of ALK, cMet, and ROS1 inhibitors was determined in CCA cell lines. To determine whether ceritinib could affect primary CCA cells, tissue was taken from four patients with biliary tract cancer, without ALK rearrangement, mutation, or overexpression, and grown in three-dimensional tumor growth assays in the presence or absence of humanized mesenchymal cells.ResultsALK and cMet but not ROS were both upregulated in CCA tissues and cell lines. Cell survival was inhibited by crizotinib, a c-met/ALK/ROS inhibitor. To determine the mechanism of this effect, we tested c-Met-specific and ALK/ROS-specific inhibitors, capmatinib and ceritinib, respectively. Whereas capmatinib did not affect cell survival, ceritinib dose-dependently inhibited survival in all cell lines, with IC50 ranging from 1 to 9 µM and co-treatments with gemcitabine and cisplatin further sensitized cells, with IC50 ranging from IC50 0.60 to 2.32 µM. Ceritinib did not inhibit cMet phosphorylation but did inhibit ALK phosphorylation. ALK was not mutated in any of these cell lines. Only ceritinib inhibited 3D growth of all four patient samples below mean peak serum concentration, in the presence and absence of mesenchymal cells, whereas crizotinib and capmatinib failed to do this. Ceritinib appeared to exert its effect more through autophagy than apoptosis.DiscussionThese results indicate that ceritinib or other ALK/ROS inhibitors could be therapeutically useful in cholangiocarcinoma even in the absence of aberrant ALK/ROS1 expression.
Correlation between long non-coding RNA MAFG-AS1 and cancer prognosis: a meta-analysis
Authors: Lin Guangyao , Liu Huicong , Lin Jingyu , Liu Xiyu , Xu Lianwei 
Published: December 2023

BackgroundMAF transcription factor G antisense RNA 1 (MAFG-AS1), a novel long non-coding RNA discovered recently, was proved to be useful in predicting malignancy prognosis. Nevertheless, its association with cancer prognosis has been inconsistent. Therefore, this meta-analysis aimed to explore the clinicopathological and prognostic significance of MAFG-AS1 in diverse carcinomas.MethodsStudies focused on MAFG-AS1 expression as a prognostic role in cancers were thoroughly searched in six electronic databases. The value of MAFG-AS1 in malignancies was assessed by hazard ratios (HRs) or odds ratios (ORs). Additionally, the GEPIA database was utilized to further strengthen our conclusion.ResultsA total of 15 studies involving 1187 cases and nine types of cancers were recruited into this meta-analysis. High MAFG-AS1 expression was significantly related to advanced tumor stage (OR = 0.52, 95%CI [0.39, 0.69], P &lt; 0.00001), earlier lymph node metastasis (OR = 3.62, 95%CI [2.19, 5.99], P &lt; 0.00001), worse tumor differentiation (OR = 0.64, 95%CI [0.43, 0.95], P = 0.03), and poor overall survival (HR = 1.94, 95%CI [1.72, 2.19], P &lt; 0.00001). No significant heterogeneity and publication bias was detected across studies. Meanwhile, MAFG-AS1 was significantly elevated in ten kinds of cancers based on the validation of the GEPIA database.ConclusionThe results of this meta-analysis indicated that high MAFG-AS1 expression is dramatically correlated with unfavorable prognosis in cancers. MAFG-AS1 may be served as a promising biomarker for malignancies.
Antinociceptive and antiinflammatory activities of crude leave extract and solvent fractions of Commelina latifolia Hochst. ex C.B.Clarke (Commelinaceae) leaves in murine model
Authors: Tadege Getnet , Sirak Betelhem , Abebe Dehnnet , Nureye Dejen 
Published: December 2023

Ethnopharmacological relevance: In the past, Ethiopian traditional medicine employed the leaves of the native Commelina latifolia Hochst. ex C.B. Clarke plant to treat wounds, pain, and malaria.Aim of the study: The crude extract and solvent fractions of C. latifolia Hochst. ex C.B. Clarke leaves were examined in the present investigation to determine their ability to have an antiinflammatory effect and provide an antinociceptive effect in animal models.Materials and methods: The leaves of C. latifolia were extracted with 80% methanol, and the CL crude extract was further fractionated with chloroform, pure methanol, and distilled water. The carrageenan-induced paw edema model was used to test the extracts’ ability to reduce inflammation. The hotplate model and the acetic acid-induced writhing test on rodents were used to test the extracts’ potential antinociceptive effect to reduce pain.Results: Inflammation was decreased by 64.59% with CL crude extract (400 mg/kg); 56.34% (400 mg/kg) of methanol fraction, 64.59% of aqueous fraction (400 mg/kg), and 38.27% of chloroform fraction in the carrageenan-induced inflammatory model. All extracts demonstrated a considerable lengthening of the nociception reaction time in the hot plate test, with a maximum antinociceptive effect of 78.98% (crude extract) and 71.65% (solvent fractions). At a dosage of 400 mg/kg, the natural C. latifolia crude extract and aqueous fraction demonstrated considerable antinociceptive effects against acetylsalicylic acid (ASA) during the writhing test (48.83% and 45.37than%, respectively). The current findings support Ethiopia’s traditional user’s assertions that the herb can alleviate inflammation and pain.
Metabolic patterns of sweat-extracellular vesicles during exercise and recovery states using clinical grade patches
Authors: Ali Nsrein , Rahat Syeda Tayyiba , Mäkelä Mira , Nasserinejad Maryam , Jaako Tommi , Kinnunen Matti , Schroderus Jyrki , Tulppo Mikko , Nieminen Anni I. , Vainio Seppo 
Published: December 2023

Background: Metabolite-based sensors are attractive and highly valued for monitoring physiological parameters during rest and/or during physical activities. Owing to their molecular composition consisting of nucleic acids, proteins, and metabolites, extracellular vesicles (EVs) have become acknowledged as a novel tool for disease diagnosis. However, the evidence for sweat related EVs delivering information of physical and recovery states remains to be addressed.Methods: Taking advantage of our recently published methodology allowing the enrichment and isolation of sweat EVs from clinical patches, we investigated the metabolic load of sweat EVs in healthy participants exposed to exercise test or recovery condition. -Ten healthy volunteers (-three females and -seven males) were recruited to participate in this study. During exercise test and recovery condition, clinical patches were attached to participants’ skin, on their back. Following exercise test or recovery condition, the patches were carefully removed and proceed for sweat EVs isolation. To explore the metabolic composition of sweat EVs, a targeted global metabolomics profiling of 41 metabolites was performed.Results: Our results identified seventeen metabolites in sweat EVs. These are associated with amino acids, glutamate, glutathione, fatty acids, creatine, and glycolysis pathways. Furthermore, when comparing the metabolites’ levels in sweat EVs isolated during exercise to the metabolite levels in sweat EVs collected after recovery, our findings revealed a distinct metabolic profiling of sweat EVs. Furthermore, the level of these metabolites, mainly myristate, may reflect an inverse correlation with blood glucose, heart rate, and respiratory rate levels.Conclusion: Our data demonstrated that sweat EVs can be purified using routinely used clinical patches during physical activity, setting the foundations for larger-scale clinical cohort work. Furthermore, the metabolites identified in sweat EVs also offer a realistic means to identify relevant sport performance biomarkers. This study thus provides proof-of-concept towards a novel methodology that will focus on the use of sweat EVs and their metabolic composition as a non-invasive approach for developing the next-generation of sport wearable sensors.
Assessment of mortality risks due to a strong cold spell in 2022 in China
Authors: Wang Wanci , Ma Yuxia , Qin Pengpeng , Liu Zongrui , Zhao Yuhan , Jiao Haoran 
Published: December 2023

BackgroundWith the intensification of global climate warming, extreme low temperature events such as cold spells have become an increasingly significant threat to public health. Few studies have examined the relationship between cold spells and mortality in multiple Chinese provinces.MethodsWe employed health impact functions for temperature and mortality to quantify the health risks of the first winter cold spell in China on November 26th, 2022, and analyzed the reasons for the stronger development of the cold spell in terms of the circulation field.ResultsThis cold spell was a result of the continuous reinforcement of the blocking high-pressure system in the Ural Mountains, leading to the deepening of the cold vortex in front of it. Temperature changes associated with the movement of cold fronts produced additional mortality risks and mortality burdens. In general, the average excess risk (ER) of death during the cold spell in China was 2.75%, with a total cumulative excess of 369,056 deaths. The health risks associated with temperatures were unevenly distributed spatially in China, with the ER values ranging from a minimum of 0.14% to a maximum of 5.72%, and temperature drops disproportionately affect southern regions of China more than northern regions. The cumulative excess deaths exibited the highest in eastern and central China, with 87,655 and 80,230 respectively, and the lowest in northwest China with 27,474 deaths. Among the provinces, excess deaths pronounced the highest in Shandong with 29,492 and the lowest in Tibet with only 196.ConclusionThe study can provide some insight into the mortality burden of cold spells in China, while emphasising the importance of understanding the complex relationship between extreme low temperature events and human health. The outcomes could provide valuable revelations for informing pertinent public health policies.
Bioinput Inoculation in Common Beans to Mitigate Stresses Caused by a Period of Drought
Authors: Arruda Bruna , Bejarano-Herrera Wilfrand Ferney , Ortega-Cepeda Maria Camila , Campo-Quesada Jose Manuel , Toro-Tobón Gabriela , Estrada-Bonilla German Andres , Silva Antonio Marcos Miranda , Ferrari Putti Fernando 
Published: December 2023

Drought conditions have made it difficult for farmers to ensure the productivity of their crops. The objective of this study was to evaluate the potential of bioinputs in stress mitigation after a drought event in common beans. Two experiments were set up in a greenhouse. Firstly, two types of soils (clayey and sandy loam) were used. After seedling emergence, treatments were set: no bacteria inoculation (NB) and inoculation with Herbaspirillum frisingense AP21. Then, a differentiation on the irrigation (15 days) was performed with no water restriction (NWR) and with water restriction (WWR). Transpiration, stomatal conductance, leaf dry matter and proline were measured. Secondly, in the clayey soil, the bacteria treatments were NB, Herbaspirillum frisingense AP21, Rhizobium leguminosarum T88 and co-inoculation (AP21 + T88). A differentiation on the irrigation (15 days) was performed: NWR and WWR. Then, Fv/Fm, photosynthetic rate, proline and sugars were assessed, and the harvest occurred 97 days after emergence. For sandy loam soil bioinputs, they did not have an effect on the parameters evaluated. For clayey soil, H. frisingense AP21 increased the transpiration rate and stomatal conductance and hence improved the leaf dry matter in comparison to NB. Under WWR, the isolated inoculations of AP21 and T88 increased grain dry matter, but the co-inoculation showed low grain production, similar to no bacteria inoculation.
Produção científica brasileira de teses e dissertações sobre saúde indígena entre 1996 a 2018
Authors: Almeida Natalia Rodrigues de , Ogata Marcia Niituma 
Published: December 2023

A pesquisa tem o objetivo de mapear a produção cientifica brasileira sobre saúde indígena analisando teses e dissertações recuperadas do Catálogo da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. A bibliometria foi utilizada para coleta e sistematização dos dados que resultaram nos seguintes indicadores: ano, instituições, programas de pós-graduação e palavras-chave. Em relação ao ano, analisou-se o período de 1996 a 2018. Os resultados demonstram que ao longo do período analisado foram defendidos 251 trabalhos sendo: 59 teses de doutorado e 192 dissertações de mestrado. As teses e dissertações foram defendidas em 162 programas de pós-graduação de 57 instituições brasileiras. A Instituição com maior número de pesquisas foi a Fundação Oswaldo Cruz que se destaca com 2 programas de pós-graduação com maior número de pesquisas defendidas, sendo saúde pública e epidemiologia em saúde pública. Em relação às palavras-chave, as 4 que tiveram maior frequência foram: saúde bucal, enfermagem, tuberculose e estado nutricional.
Leituras CTS da Ciência e da Modernidade
Authors: Oliveira Maysa Leal de 
Published: December 2023

A atual crise climática global evidencia os limites e equívocos do pensamento ocidental moderno, convidando a uma reflexão crítica sobre seu desenvolvimento, que demonstre as correlações que mantém com o atual estado das coisas. Sob o enfoque dos Estudos de Ciência, Tecnologia e Sociedade (CTS), retraçamos, numa compreensão sócio-histórica renovada, o percurso de desenvolvimento da ciência europeia moderna e do paradigma da modernidade, recorrendo às contribuições da filosofia, sociologia e história das ciências para desnaturalizar sua suposta superioridade epistêmica e questionar os seus fundamentos e hegemonia. O texto também dialoga com os estudos pós-coloniais, com pensadores da cultura brasileira e autores indígenas do Brasil, realçando a necessidade de uma emancipação epistêmica e da descolonização do pensamento, no sentido de um diálogo não hierárquico com os saberes da tradição.
Does a flashing artificial light have more or conversely less impacts on animals than a continuous one? A systematic review
Authors: Lafitte Alix , Sordello Romain , Legrand Marc , Nicolas Virginie , Obein Gaël , Reyjol Yorick 
Published: December 2023

Background: Light pollution has been increasingly recognised as a threat to biodiversity, especially with the current expansion of public lighting. Although the impacts of light intensity, spectral composition and temporality are more often studied, another component of light, its flicker frequency, has been largely overlooked. However, flashing light could also have impacts on biodiversity, and especially on animal behaviour and physiology.
Objective: This systematic review aimed at identifying the reported physiological and behavioural impacts of flashing light on animals when compared to continuous light.
Methods: We followed the standards recommended by the Collaboration for Environmental Evidence (CEE) in order to achieve a comprehensive, transparent and replicable systematic review. Citations were primarily extracted from three literature databases and were then screened for relevance successively on their titles, abstracts and full-texts. Retained studies were finally critically appraised to assess their validity and all relevant data were extracted. Only studies which compared a flashing light to a continuous one were included.
Results: At first, we found 19,730 citations. Screening and critical appraisal resulted in 32 accepted articles corresponding to 54 accepted observations—one observation corresponding to one species and one outcome. We collated data on four main taxa: Aves (the most studied one), Actinopterygii, Insecta and Mammalia as well as on plankton.
Conclusions: The impacts of flashing light are currently critically understudied and varied between species and many light specificities (e.g. frequency, wavelength, intensity). Therefore, no definitive conclusions could be drawn for now. Thus, research on flashing light should be pressingly carried out in order to better mitigate the impacts of Artificial Light at Night (ALAN) on wildlife. In the meantime, we would recommend precautionary principles to be applied: flashing lighting should be limited when not deemed essential and flicker frequencies managed to prevent animals from experiencing any potential harm from flashing light.
L’antispiritualité d’Alexandra David-Neel
Authors: Dapsance Marion 
Published: December 2023

Parce qu’elle s’est déclarée bouddhiste, s’est rendue à Lhassa et a beaucoup écrit sur le Tibet, Alexandra David-Neel (1868-1969) passe souvent pour un « maître spirituel », une « mystique », une adepte des religions tibétaines. Pourtant, le bouddhisme auquel elle souscrit est dénué de toute transcendance et de la pratique de rituels. Il doit bien plus à l’influence des théosophes britanniques qu’à celle des maîtres asiatiques.
Michel Serres ou la « science des passerelles »
Authors: Klein Étienne 
Published: December 2023

Michel Serres (1930-2019) est décédé le 1 er juin à l’âge de 88 ans. Il fut un philosophe atypique, escaladant joyeusement les murs que l’Académie aime tant à construire. Voyageant entre les sciences dures et les sciences humaines, il inventa une « science des passerelles » qui réconcilia la culture scientifique et la culture littéraire, en ouvrant à la pensée des chemins inédits.
La Fondation Rockefeller, les sciences politiques et l’Europe : la circulation d’un savoir stratégique durant la guerre froide
Authors: Attal Frédéric 
Published: December 2023

Cet article aborde l’introduction des sciences politiques dans les universités italiennes sur l’instigation des fondations philanthropiques étatsuniennes. Il entend démontrer que si le contexte politique et international de Guerre froide n’est pas étranger à l’intérêt que les fondations éprouvent pour ce « savoir stratégique », il serait erroné de conclure que son développement serait une greffe imposée par les États-Unis dans le cadre d’une américanisation supposée du champ académique européen.
L’Égypte et ses crises : too big to fail ?
Authors: Salama Joseph 
Published: December 2023

À la veille d’une élection présidentielle dont l’issue ne fait guère de doute, l’Égypte est engluée dans une crise économique majeure. Les ingrédients de cette crise sont nombreux : impact du Covid-19 sur le tourisme, conséquences de la guerre en Ukraine, mesures imposées par le Fonds monétaire international, clientélisme du régime… Le pays est de plus en plus dépendant des monarchies du Golfe, mais leur aide ne va pas sans contreparties. L’avenir de l’Égypte paraît bien sombre.
Quand les prénoms parlent… De l’original à l’originel en clinique de l’infertilité
Authors: Agüero Anne , Bydlowski Sarah 
Published: December 2023

Alors que le recours à l’Assistance Médicale à la Procréation médicalement (AMP) est de plus en plus fréquent, les études parues donnent des résultats contradictoires sur le devenir et le développement des enfants ainsi conçus. Dans le cadre d’une recherche longitudinale, exploratoire, quantitative et qualitative menée à 3 mois, 2 ans et 4 ans, nous proposons de nous intéresser aux prénoms donnés. Nous faisons en effet l’hypothèse, que le choix du prénom est non seulement un miroir du vécu d’étrangeté ressenti par les parents, mais qu’il contient en germes, les premières interactions avec leur part de rêverie, de fantasmes et de dette parentale. La méthodologie est constituée d’entretiens filmés, analysés selon quatre axes principaux : la narration spontanée et manifeste du choix du prénom, la préexistence du prénom à la grossesse, le prénom comme choix commun parental, l’inscription du prénom dans une filiation. Notre objectif est de pouvoir dégager de notre cohorte de 70 familles des invariants concernant les éventuelles fragilités psychiques qui pourraient découler d’une AMP afin de mettre en place des actions de prévention.
Prendre son temps à l’adolescence, entre élaboration diagnostique et clinique du deuil pathologique :
Authors: Bringer Marie , Ludot Maude , Roy Edward Tony , Follet Nathalie , Hanin Cyril , Harf Aurélie , Moro Marie Rose 
Published: December 2023

L’adolescence est une période charnière sur le plan développemental, tant elle est marquée par des mouvements psychiques intenses, paradoxaux et complexes à appréhender et à comprendre par l’adolescent qui en est traversé. L’adolescent se heurte à des étapes psychiques importantes qui entrainent une symptomatologie anxieuse reflétant tout ce travail de séparation-individuation. Les hôpitaux de jour constituent un lieu privilégié d’observation clinique et d’expérimentation de liens au groupe des pairs. Les médiations thérapeutiques proposées permettent alors l’accès à différents niveaux et registres psychiques tout en étant soutenus par la contenance groupale. Nous aborderons la clinique du deuil pathologique de l’adolescent illustrée par la situation clinique d’Emma, une adolescente de 16 ans, dont l’émancipation psychique a été rendue possible.