bluuebunny/crossref_metadata_2025_split huggingface dataset:
Competitive MS Binding Assays for Dopamine D2Receptors Employing Spiperone as a Native Marker
Authors: Niessen Karin V. , Höfner Georg , Wanner Klaus T. 
Published: September 2005

AbstractA competitive MS binding assay employing spiperone as a native marker and a porcine striatal membrane fraction as a source for dopamine D2receptors in a nonvolatile buffer has been established. Binding of the test compounds to the target was monitored by mass‐spectrometric quantification of the nonbound marker, spiperone, in the supernatant of the binding samples obtained by centrifugation. A solid‐phase extraction procedure was used for separating spiperone from ESI‐MS‐incompatible supernatant matrix components. Subsequently, the marker was reliably quantified by LC‐ESI‐MS‐MS by using haloperidol as an internal standard. The affinities of the test compounds, the dopamine receptor antagonists (+)‐butaclamol, chlorpromazine and (S)‐sulpiride obtained from the competitive MS binding assay were verified by corresponding radioligand binding experiments with [3H]spiperone. The results of this study demonstrate that competitive MS binding assays represent a universally applicable alternative to conventional radioligand binding assays.
Probing Lectin and Sperm with Carbohydrate‐Modified Quantum Dots
Authors: Robinson Anandakathir , Fang Jim‐Min , Chou Pi‐Tai , Liao Kuang‐Wen , Chu Rea‐Min , Lee Shyh‐Jye 
Published: September 2005

AbstractWe report the encapsulation of quantum dots with biologically important β‐N‐acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc‐encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose‐encapsulated QDs (QDMANs) with sperm revealed site‐specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.
Core values and major issues in student affairs practice: What really matters?
Authors: Helfgot Steven R. 
Published: September 2005

AbstractIn a professional world characterized by change, uncertainty, and increased pressure to demonstrate that what they do really matters, community college student affairs professionals need clear definitions, a shared understanding of critical professional issues, and professional values that are consistent and congruent. This chapter provides those definitions and identifies core values and major issues for student affairs professionals in community colleges.
Increasing the value of traditional support services
Authors: Culp Marguerite M. 
Published: September 2005

AbstractStudent affairs practitioners must reconceptualize traditional support services in order to use the resources allocated to them more effectively, meet the needs of today's students, and increase their value to community colleges. This chapter identifies student affairs programs that are vital to the community college's mission, describes best practices in community college student affairs, and demonstrates why traditional support services are essential to the success of the contemporary community college.
Connecting academic and student affairs to enhance student learning and success
Authors: Dale Paul A. , Drake Tonya M. 
Published: September 2005

AbstractAs community colleges embrace learning‐centered practices, student affairs professionals have an opportunity to recommit to building and maintaining partnerships with their academic colleagues. This chapter explores the development, nature, and culture of learning‐centered partnerships between academic and student affairs professionals, identifies best practices in community colleges, and examines the role of technology in enhancing partnership opportunities.
Doing more of what matters: The key to student success
Authors: Culp Marguerite M. 
Published: September 2005

AbstractThis chapter identifies eleven factors that, when present in a community college, substantially increase the probability that student affairs professionals will be able to create programs that matter to the institution‐s core mission. The chapter also provides student affairs practitioners with tools to assess the climate in student affairs as well as in the institution for both students and student affairs programs. Finally, the chapter offers guidelines that practitioners can use to design student affairs programs that matter.
Dynamic Id2 expression in the medial and lateral domains of avian dermamyotome
Authors: Krishan K. , McKinnell I. , Patel K. , Dhoot G.K. 
Published: September 2005

AbstractId2 cDNA was isolated from a subtractive screen of stage‐12 quail caudal somites. In situ hybridisation analysis identified the previously un‐described expression of Id2 mRNA in distinct medial and lateral domains of the somitic dermamyotome in both quail and chick embryos. Id2 expression in somites was highly dynamic being first initiated in the lateral domain of the dermamyotome of stage‐8–10 embryos, followed by expression in a separate medial domain. Id2 mRNA during subsequent embryonic development could be detected in both medial and lateral domains in the anterior to mid regions while the posterior, recently segmented somites, showed expression only in the lateral domain, which was eventually down regulated in the anterior‐most somites. Tissue manipulation studies revealed that Id2 expression in somites required positive signalling from not only axial structures and lateral plate mesoderm but also surface ectoderm. In addition, Id2 expression was also observed in anterior and posterior domains of developing avian limb buds and interdigital tissue. Developmental Dynamics 234:363–370, 2005. © 2005 Wiley‐Liss, Inc.
A Theoretical Assessment of the Thermodynamic Preferences in the Cyclopalladation of Amines
Authors: Bosque Ramón , Maseras Feliu 
Published: September 2005

AbstractThe factors affecting the cyclopalladation of amines are explored by a comparison of the energy differences between the cyclopalladated amine and the free ligand for a series of compounds. Four factors are considered: the substituents in the nitrogen atom (primary vs. tertiary amines), the hybridization (sp2 or sp3) of the metalated carbon atom, the number of members in the metallacycle ring (four, five, or six), and the presence of electron‐donor or ‐acceptor groups. The trends that arise from these calculations, performed at the B3LYP level of theory, are compared with the experimental data available. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
New Polysulfur‐Nitrogen Heterocycles by Thermolysis of 1,3λ4δ2,2,4‐Benzodithiadiazines in the Hydrocarbon and Fluorocarbon Series
Authors: Zhivonitko Vladimir V. , Makarov Alexander Yu. , Bagryanskaya Irina Yu. , Gatilov Yuri V. , Shakirov Makhmut M. , Zibarev Andrey V. 
Published: September 2005

AbstractIn contrast to thermolysis of 1,3λ4δ2,2,4‐benzodithiadiazine (1) and its 5,6,7,8‐tetrafluoro derivative 2 in dilute (10–3 M) hydrocarbon solutions, which leads to persistent 1,2,3‐benzodithiazolyls in nearly quantitative yields, the thermolysis of 1 and 2 (the 6‐6 bicyclic system) in concentrated (0.5 M) solutions at 150–170 °C results in complex mixtures of various polysulfur‐nitrogen heterocycles, in particular differently fused 5‐5‐6, 5‐6‐7 and 5‐6‐6‐6 polycyclic systems that were previously unknown. The products were isolated by column chromatography, and the structures of the 5‐5‐6 (11, 12), 5‐6‐7 (5, 13), and 5‐6‐6‐6 (6, 14, 15) polycyclic systems, as well as the 6‐7 (4) and 5‐6 (16) bicyclic derivatives, confirmed by X‐ray diffraction. The crystal packing of 6 and 11–16 is discussed with special emphasis on π‐stacking interactions. The fluorescent properties of the 5‐6‐6‐6 systems synthesized are reported. An alternative approach to the linear 5‐6‐6‐6 system 15 based on self‐condensation of an R–N=PPh3 precursor (R = 4,5,6,7‐tetrafluoro‐1,2,3‐benzodithiazol‐2‐yl) is described, and the free‐radical character of this new reaction is proved. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
A New ZnII Tweezer Pyridine‐Naphthalene System – An Off‐On‐Off System Working in a Biological pH Window
Authors: Aucejo Ricardo , Alarcón Javier , García‐España Enrique , Llinares José M. , Marchin Katherine L. , Soriano Conxa , Lodeiro Carlos , Bernardo M. Alexandra , Pina Fernando , Pina João , Seixas de Melo J. 
Published: September 2005

AbstractThe synthesis, protonation behaviour and Zn2+ coordination chemistry of a new photoactive polyamine exhibiting “off‐on‐off” fluorescence is presented. Addition of Zn2+ reduces the width of the fluorescence window, which is centred around physiological pH, and sharply enhances the emission intensity. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Anions of 1,3,5‐Benzenetricarboxylic and Heptanedioic Acids Serving as Bridges between Dimolybdenum(V) Metal–Metal Bonded Units: Preparation and Structural Characterization of Dinuclear and Tetranuclear Complexes
Authors: Modec Barbara , Brenčič Jurij V. 
Published: September 2005

Abstract(PyH)5[MoOCl4(H2O)]3Cl2 and (PyH)[MoOBr4] reacted with the anions of 1,3,5‐benzenetricarboxylic acid (btcH3) and heptanedioic acid (hdaH2) to afford a series of novel carboxylato complexes based on the {Mo2O4}2+ structural core: (PyH)4[Mo2O4Cl4(μ2‐btcH2)]Cl (1), (PyH)4[Mo2O4Br4(μ2‐btcH2)]Br (2), [(Mo2O4Py3)2(μ3‐btcH)2]·6Py (3), (PyH)6[(Mo2O4Cl4)2(μ4‐hda)]·2CH3CN (4), and (PyH)10[(Mo2O4Cl4)2(μ4‐hda)][MoOCl5]Cl2 (5) {Py = pyridine, C5H5N; PyH+ = pyridinium cation, C5H5NH+; btcH2– = (HOOC)2C6H3COO–; btcH2– = (HOOC)C6H3(COO)22– and hda2– = –OOC(CH2)5COO–}. A pair of isostructral compounds 1 and 2 contains dinuclear anions [Mo2O4X4(μ2‐btcH2)]3– (X = Cl, Br) in which the btcH2– ligand is bonded to the {Mo2O4}2+ core via one carboxylate group in a syn‐syn bidentate bridging manner. In 3, two btcH2– ligands link two {Mo2O4}2+ cores into a loop‐like structure. Each btcH2– ligand is coordinated via two carboxylate groups, with one in the syn‐syn bidentate bridging manner and with another in a monodentate manner. The linkage of two dinuclear subunits was also realized in [(Mo2O4Cl4)2(μ4‐hda)]6–, the anion in 4 and 5, where both carboxylate groups of the hda2– ligand bind in the syn‐syn bidentate‐bridging manner to {Mo2O4}2+ cores. The title compounds were characterized by X‐ray crystallography and infrared spectroscopy. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
New Chiral Building Blocks and Branched 1,6‐Anhydro Sugars from Regio‐ and Stereoisomeric Černý Epoxides
Authors: Krohn Karsten , Gehle Dietmar , Flörke Ulrich 
Published: September 2005

AbstractThe tandem epoxide→allyl alcohol rearrangement–cuprate cross‐coupling previously described for the Černý epoxide 1, to yield the allyl alcohol 2, was extended to the regioisomeric epoxy‐tosylate 3, to yield allyl alcohol 4, and to the stereoisomeric epoxide 5 to afford the allyl alcohols of type 6. Further transformation of the products from this new two‐step one‐pot reaction afforded regio‐ and stereodiverse branched 1,6‐anhydrosugars of potential use as building blocks in natural product synthesis. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Molecular Selective Binding of Pyridinium Guest Ions by Water‐Soluble Calix[4]arenes
Authors: Liu Yu , Yang En‐Cui , Chen Yong , Guo Dong‐Sheng , Ding Fei 
Published: September 2005

AbstractThe complex stability constants (KS) and thermodynamic parameters (ΔG°, ΔH°, and TΔS°) for 1:1 intermolecular complexation of water‐soluble calix[4]arene tetrasulfonate (CAS) and thiacalix[4]arene tetrasulfonate (TCAS) with pyridinium guest ions 1–10 have been determined by means of titration calorimetry in an acidic buffer solution (pH = 2.0) at 298.15 K, and their binding modes have been investigated by NMR spectroscopy. Possessing a smaller cavity and a higher π electron density than TCAS, CAS affords the stronger binding abilities for all guests examined, giving an exciting molecular selectivity of up to 354 for the 2,6‐dimethylpyridine/2,6‐pyridinedicarboxylic acid pair. The high binding affinities, attributed to the favorable enthalpic gains, are comprehensively discussed from the viewpoint of π–π stacking, electronic effect and size‐fit relationship between host and guest. The thermodynamic parameters that have been obtained, together with the NMR results, jointly demonstrate that the position, number, and type of substituent groups introduced onto the guest molecule are the key factors controlling the structural‐energetics correlation for the molecular selective binding of water‐soluble calixarenes. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
A New Modular and Practical Methodology for the Synthesis of 4‐ or 3‐Substituted Phenyl C‐Nucleosides
Authors: Hocek Michal , Pohl Radek , Klepetářová Blanka 
Published: September 2005

AbstractA novel efficient and practical approach to the synthesis of 4‐ or 3‐substituted phenyl C‐nucleosides has been developed. It consists in coupling of protected halogenose 1 with bromophenylmagnesium bromides followed by acid mediated epimerization to prepare 4‐ or 3‐bromophenyl C‐nucleoside intermediates. Their Pd‐catalyzed cross‐coupling reactions with diverse organometallics followed by deprotection afforded the title nucleoside analogues. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Information rate bounds in common‐mode aided wireline communications
Authors: Magesacher Thomas , Ödling Per , Börjesson Per Ola , Shamai (Shitz) Shlomo 
Published: September 2005

AbstractCommunication over the copper twisted‐pair channel is performed by transmitting and receiving differential‐mode (DM) signals. In this paper, we extend the conventional DM receiver scheme by incorporating the common‐mode (CM) signal, which is strongly correlated with the DM signal and can be extracted at the end of every wire pair. The wireline channel, including the CM receive signal, is modelled as a Gaussian vector (multiple‐input multiple‐output) channel with memory and an arbitrary number of disturbers that introduce interference which can be correlated both temporally and spatially. We investigate the potential of CM‐aided reception in terms of information rate for single‐user digital subscriber line systems (xDSL) operating on a single pair. Numerical results of information rate calculations using measured channel data are presented. The achievable information rate of the twisted‐pair channel when incorporating the CM signal in practically relevant example scenarios can exceed the information rate of the conventional DM channel by a factor of up to two. Copyright © 2005 AEIT.
Effect of an anti‐CD54 (ICAM‐1) monoclonal antibody (UV3) on the growth of human uveal melanoma cells transplanted heterotopically and orthotopically in SCID mice
Authors: Wang Shixuan , Coleman Elaine J. , Pop Laurentiu M. , Brooks Kimberly J. , Vitetta Ellen S. , Niederkorn Jerry Y. 
Published: September 2005

AbstractWe have shown that administration of a novel anti‐CD54 monoclonal antibody (UV3) results in long‐term survival of SCID mice bearing human myeloma xenografts. Previous studies have demonstrated a link between the expression of CD54 and the progression of uveal melanoma. Our study assessed the expression of CD54 on 7 human uveal melanoma cell lines and 3 cell lines established from uveal melanoma metastases. In vivo studies examined the efficacy of systemic and local administration of UV3 antibody on the progression of uveal melanoma cells transplanted either heterotopically or orthotopically into SCID mice. Five of the 7 primary uveal melanoma cell lines and all 3 of the metastases cell lines expressed CD54. Intraperitoneal injection of either IgG or F(ab′)2 fragments of UV3 significantly inhibited the growth of subcutaneous and intraocular melanomas. Subconjunctival injection of either IgG or F(ab′)2 fragments of UV3 produced a significant reduction in the growth of intraocular melanomas, even if the antibody was administered after the appearance of intraocular tumors. The results indicate that both primary and metastatic human uveal melanoma cells express CD54. The marked inhibition of intraocular and subcutaneous uveal melanoma progression suggests that UV3 antibody is a promising therapeutic agent for further evaluation in patients with uveal melanoma. This is especially noteworthy, as no existing therapeutic modality prevents metastasis of uveal melanoma or prolongs the survival of patients with uveal melanoma. © 2005 Wiley‐Liss, Inc.
DNA methylation patterns in adenomas from FAP, multiple adenoma and sporadic colorectal carcinoma patients
Authors: Wynter Coral V.A. , Kambara Takeshi , Walsh Michael D. , Leggett Barbara A. , Young Joanne , Jass Jeremy R. 
Published: September 2005

AbstractColorectal adenomas have traditionally been regarded as homogeneous. The aim of our study was to identify molecular features that may differentiate sporadic adenomas from familial adenomas such as Familial Adenomatous Polyposis (FAP) and Multiple Adenoma patients. DNA methylation was tested at Methylated IN Tumor (MINT) loci (1,2,12,31) and the CpG promoter region of genes MLH1, HPP1, MGMT, p14ARF and p16INK4a in FAP‐associated adenomas (33) from 5 patients with a known APC mutation (Group 1, FAP), adenomas (29) from 4 Multiple Adenoma patients (Group 2 Multiple), adenomas (14) from 3 patients with sporadic colorectal cancers showing high microsatellite instability (Group 3, MSI‐H) and adenomas (16) from 7 patients, with sporadic colorectal cancers showing microsatellite stable or low level instability (Group 4, MSS/MSI‐L). Aberrant Crypt Foci (ACFs), Hyperplastic Polyps (HPs) and cancers were also examined for methylation status as well as K‐ras mutation. Multiple Adenoma patients were examined for germline polymorphisms in the base excision repair gene, MYH. The familial syndrome, FAP ‐associated adenomas showed a significantly low frequency of MINT methylation (15.5%,) compared to sporadic MSS/MSI‐L‐associated adenomas (35.5%). Group 3 (MSI‐H) adenomas were different in that many showed serration and a high level of methylation (57.1%). Group 2, Multiple Adenoma cases, resembled sporadic MSS/MSI‐L‐associated adenomas. However the promoter regions of key genes, MGMT, p14ARF and p16INK4a were methylated to a greater extent than MINTs in both sporadic and familial adenomas. Genetic profiling of adenomas supports the concept that adenomas belonging to familial syndromes pursue a different pathway to tumorigenesis than their sporadic counterpar/ts from their earliest formation. © 2005 Wiley‐Liss, Inc.
Loss of intercellular adhesion activates a transition from low‐ to high‐grade human squamous cell carcinoma
Authors: Margulis Alexander , Zhang Weitian , Alt‐Holland Addy , Pawagi Sujata , Prabhu Padmaja , Cao Jian , Zucker Stanley , Pfeiffer Laurence , Garfield Jacqueline , Fusenig Norbert E. , Garlick Jonathan A. 
Published: September 2005

AbstractThe relationship between loss of intercellular adhesion and the biologic properties of human squamous cell carcinoma is not well understood. We investigated how abrogation of E‐cadherin‐mediated adhesion influenced the behavior and phenotype of squamous cell carcinoma in 3D human tissues. Cell‐cell adhesion was disrupted in early‐stage epithelial tumor cells (HaCaT‐II‐4) through expression of a dominant‐negative form of E‐cadherin (H‐2Kd‐Ecad). Three‐dimensional human tissue constructs harboring either H‐2Kd‐Ecad‐expressing or control II‐4 cells (pBabe, H‐2Kd‐EcadΔC25) were cultured at an air‐liquid interface for 8 days and transplanted to nude mice; tumor phenotype was analyzed 2 days and 2 and 4 weeks later. H‐2Kd‐Ecad‐expressing tumors demonstrated a switch to a high‐grade aggressive tumor phenotype characterized by poorly differentiated tumor cells that infiltrated throughout the stroma. This high‐grade carcinoma revealed elevated cell proliferation in a random pattern, loss of keratin 1 and diffuse deposition of laminin 5 γ2 chain. When II‐4 cell variants were seeded into type I collagen gels as an in vitro assay for cell migration, we found that only E‐cadherin‐deficient cells detached, migrated as single cells and expressed N‐cadherin. Function‐blocking studies demonstrated that this migration was matrix metalloproteinase‐dependent, as GM‐6001 and TIMP‐2, but not TIMP‐1, could block migration. Gene expression profiles revealed that E‐cadherin‐deficient II‐4 cells demonstrated increased expression of proteases and cell‐cell and cell‐matrix proteins. These findings showed that loss of E‐cadherin‐mediated adhesion plays a causal role in the transition from low‐ to high‐grade squamous cell carcinomas and that the absence of E‐cadherin is an important prognostic marker in the progression of this disease. © 2005 Wiley‐Liss, Inc.
Occupational physical activity and risk of adenocarcinomas of the esophagus and stomach
Authors: Vigen Cheryl , Bernstein Leslie , Wu Anna H. 
Published: September 2005

AbstractPhysical activity may have a role in many cancers, but little is known about its effect on esophageal and gastric adenocarcinoma risk. We investigated occupational physical activity and esophageal and gastric adenocarcinoma risk in a population‐based, case‐control study including 212 esophageal, 264 gastric cardia and 389 distal gastric cancer cases, and 1,330 controls in Los Angeles County. Lifetime occupational histories were obtained during in‐person interviews, and total lifetime occupational activity (Total Activity Index) was calculated using US Census job codes classified as sedentary, or moderately or highly physically active. Average Annual Activity Index was a per‐year Total Activity Index counterpart. Unconditional logistic regression was used to calculate odds ratios, 95% confidence intervals and trend tests adjusting for gender, race, age, birthplace, education, smoking, body mass index (BMI) and number of years worked. Esophageal adenocarcinoma risk tended to decrease with increasing Total Activity Index (OR = 0.67, 95% CI = 0.38,1.19 for highest versus lowest quartile), but neither gastric cardia nor distal gastric cancer was associated with the Total Activity Index. This inverse association held for esophageal adenocarcinoma (OR = 0.61, 95% CI = 0.38,0.99 for highest vs. lowest quartile) and modest associations were observed for gastric cardia (OR = 0.76, 95% CI = 0.49,1.18) and distal gastric cancer (OR = 0.77, 95% CI = 0.52,1.14) when based on Average Annual Activity Index before age 65 years. Analyses stratified by gender, race, age, BMI, education and years worked provided similar results. We found a modest protective effect of Total Activity Index on esophageal adenocarcinoma. Future studies with more complete information on occupational and recreational physical activity are needed to confirm and further investigate the suggested protective effect of physical activity on these tumor types. © 2005 Wiley‐Liss, Inc.