bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomarker study of symptomatic intracranial atherosclerotic stenosis in patients with acute ischemic stroke
Authors: Ding Yingyue , Li Jinjian , Shan Huiyu , Yang Song , Wang Xiyuan , Zhao Dexi 
Published: December 2023

ObjectiveAcute ischemic stroke (AIS) is characterized by high rates of morbidity, disability, mortality, and recurrence, often leaving patients with varying degrees of sequelae. Symptomatic intracranial atherosclerotic stenosis (sICAS) is a significant contributor to AIS pathogenesis and recurrence. The formation and progression of sICAS are influenced by pathways such as lipid metabolism and inflammatory response. Given its high risk of clinical recurrence, timely assessment of intracranial vascular stenosis in AIS is crucial for diagnosing sICAS, treating stroke, and preventing stroke recurrence.MethodsFourteen AIS patients were divided into stenosis and control groups based on the presence or absence of intracranial vessel stenosis. Initially, 4D Label-free proteome quantification technology was employed for mass spectrometry analysis to identify differential proteins between the groups. Subsequently, functional enrichment analysis, including GO classification, KEGG pathway, and Domain, revealed trends related to differential proteins. The STRING (v.11.5) protein interaction network database was used to identify differential protein interactions and target proteins. Finally, parallel reaction monitoring (PRM) validated the selected target proteins.ResultsMass spectrometry identified 1,096 proteins, with 991 being quantitatively comparable. Using a p-value &lt;0.05 and differential expression change thresholds of &gt;1.3 for significant up-regulation and &lt; 1/1.3 for significant down-regulation, 46 differential proteins were identified: 24 significantly up-regulated and 22 significantly down-regulated. PRM experiments validated five proteins related to lipid metabolism and inflammatory response: namely alpha-2-macroglobulin (A2M), lipopolysaccharide-binding protein (LBP), cathepsin G (CTSG), cystatin (CST)3, and fatty acid-binding protein (FABP)1.ConclusionThe detection of changes in these five proteins in AIS patients can aid in the diagnosis of sICAS, inform stroke treatment, and assist in preventing stroke recurrence. Moreover, it can contribute to the development of drugs for preventing AIS recurrence by integrating traditional Chinese and Western medicine.
Systematic bibliometric and visualized analysis of research hotspots and trends in artificial intelligence in autism spectrum disorder
Authors: Jia Qianfang , Wang Xiaofang , Zhou Rongyi , Ma Bingxiang , Fei Fangqin , Han Hui 
Published: December 2023

BackgroundArtificial intelligence (AI) has been the subject of studies in autism spectrum disorder (ASD) and may affect its identification, diagnosis, intervention, and other medical practices in the future. Although previous studies have used bibliometric techniques to analyze and investigate AI, there has been little research on the adoption of AI in ASD. This study aimed to explore the broad applications and research frontiers of AI used in ASD.MethodsCitation data were retrieved from the Web of Science Core Collection (WoSCC) database to assess the extent to which AI is used in ASD. CiteSpace.5.8. R3 and VOSviewer, two online tools for literature metrology analysis, were used to analyze the data.ResultsA total of 776 publications from 291 countries and regions were analyzed; of these, 256 publications were from the United States and 173 publications were from China, and England had the largest centrality of 0.33; Stanford University had the highest H-index of 17; and the largest cluster label of co-cited references was machine learning. In addition, keywords with a high number of occurrences in this field were autism spectrum disorder (295), children (255), classification (156) and diagnosis (77). The burst keywords from 2021 to 2023 were infants and feature selection, and from 2022 to 2023, the burst keyword was corpus callosum.ConclusionThis research provides a systematic analysis of the literature concerning AI used in ASD, presenting an overall demonstration in this field. In this area, the United States and China have the largest number of publications, England has the greatest influence, and Stanford University is the most influential. In addition, the research on AI used in ASD mostly focuses on classification and diagnosis, and “infants, feature selection, and corpus callosum are at the forefront, providing directions for future research. However, the use of AI technologies to identify ASD will require further research.
A fast Monte Carlo cell-by-cell simulation for radiobiological effects in targeted radionuclide therapy using pre-calculated single-particle track standard DNA damage data
Authors: Lim A. , Andriotty M. , Yusufaly T. , Agasthya G. , Lee B. , Wang C. 
Published: December 2023

IntroductionWe developed a new method that drastically speeds up radiobiological Monte Carlo radiation-track-structure (MC-RTS) calculations on a cell-by-cell basis.MethodsThe technique is based on random sampling and superposition of single-particle track (SPT) standard DNA damage (SDD) files from a “pre-calculated” data library, constructed using the RTS code TOPAS-nBio, with “time stamps” manually added to incorporate dose-rate effects. This time-stamped SDD file can then be input into MEDRAS, a mechanistic kinetic model that calculates various radiation-induced biological endpoints, such as DNA double-strand breaks (DSBs), misrepairs and chromosomal aberrations, and cell death. As a benchmark validation of the approach, we calculated the predicted energy-dependent DSB yield and the ratio of direct-to-total DNA damage, both of which agreed with published in vitro experimental data. We subsequently applied the method to perform a superfast cell-by-cell simulation of an experimental in vitro system consisting of neuroendocrine tumor cells uniformly incubated with 177Lu.Results and discussionThe results for residual DSBs, both at 24 and 48 h post-irradiation, are in line with the published literature values. Our work serves as a proof-of-concept demonstration of the feasibility of a cost-effective “in silico clonogenic cell survival assay” for the computational design and development of radiopharmaceuticals and novel radiotherapy treatments more generally.
A tumor cell specific Zona Pellucida glycoprotein 3 RNA transcript encodes an intracellular cancer antigen
Authors: Schultz Iman J. , Zimmerman Yvette , Moelans Cathy B. , Chrusciel Marcin , Krijgh Jan , van Diest Paul J. , Huhtaniemi Ilpo T. , Coelingh Bennink Herjan J. T. 
Published: December 2023

BackgroundExpression of Zona Pellucida glycoprotein 3 (ZP3) in healthy tissue is restricted to the extracellular Zona Pellucida layer surrounding oocytes of ovarian follicles and to specific cells of the spermatogenic lineage. Ectopic expression of ZP3 has been observed in various types of cancer, rendering it a possible therapeutic target.MethodsTo support its validity as therapeutic target, we extended the cancer related data by investigating ZP3 expression using immunohistochemistry (IHC) of tumor biopsies. We performed a ZP3 transcript specific analysis of publicly available RNA-sequencing (RNA-seq) data of cancer cell lines (CCLs) and tumor and normal tissues, and validated expression data by independent computational analysis and real-time quantitative PCR (qPCR). A correlation between the ZP3 expression level and pathological and clinical parameters was also investigated.ResultsIHC data for several cancer types showed abundant ZP3 protein staining, which was confined to the cytoplasm, contradicting the extracellular protein localization in oocytes. We noticed that an alternative ZP3 RNA transcript, which we term ‘ZP3-Cancer’, was annotated in gene databases that lacks the genetic information encoding the N-terminal signal peptide that governs entry into the secretory pathway. This explains the intracellular localization of ZP3 in tumor cells. Analysis of publicly available RNA-seq data of 1339 cancer cell lines (CCLs), 10386 tumor tissues (The Cancer Genome Atlas) and 7481 healthy tissues (Genotype-Tissue Expression) indicated that ZP3-Cancer is the dominant ZP3 RNA transcript in tumor cells and is highly enriched in many cancer types, particularly in rectal, ovarian, colorectal, prostate, lung and breast cancer. Expression of ZP3-Cancer in tumor cells was confirmed by qPCR. Higher levels of the ZP3-Cancer transcript were associated with more aggressive tumors and worse survival of patients with various types of cancer.ConclusionThe cancer-restricted expression of ZP3-Cancer renders it an attractive tumor antigen for the development of a therapeutic cancer vaccine, particularly using mRNA expression technologies.
Summary of the application value of ultrasound imaging features in the clinical differential diagnosis of intramuscular capillary-type hemangioma and fibro-adipose vascular anomaly
Authors: Hu Wen-Jia , Li Hai-Ting , Fan Zhi-Na , Gong Yu-Bin , Guo Xiao-Nan , Dong Chang-Xian , Fan Pan-Hong , Yang Xiao , Wu Gang 
Published: December 2023

ObjectiveTo investigate the value of ultrasonography as a diagnostic aid in differentiating intramuscular capillary-type hemangioma (ICTH) from fibro-adipose vascular anomaly (FAVA).MethodsA retrospective analysis was conducted of the clinical and ultrasound imaging data of 20 patients with ICTH and 45 patients with FAVA who were admitted to and pathologically confirmed in hospital between January 2013 and April 2023. The clinical and ultrasonographic appearances of the lesions in the two groups were compared and analyzed. A stepwise regression analysis was performed, and a joint diagnostic equation was constructed using the final variables selected. The receiver operating characteristic (ROC) curve and indicators, including sensitivity and specificity, were used to evaluate the efficacy of the joint diagnostic model.ResultsThe two groups of patients suffering from ICTH and FAVA presented a statistically significant difference (P&lt; 0.05) in terms of ‘age’, ‘lesion size’, ‘fascial tail sign’, ‘presence of a fatty-tissue-like hyperecho around the lesion’, ‘blood flow’ and ‘presence of straight blood capillaries within the lesion’. Finally, the variables ‘fascial tail sign’ and ‘presence of straight blood capillaries within the lesion’ were selected to construct the model. The constructed joint diagnostic model had a sensitivity value of 70.0% (95% CI: 59.00–81.00), a specificity value of 98.0% (95% CI: 94.70–100.00) and a ROC curve value of 0.908, indicating the high efficacy of the combined diagnosis method.ConclusionsUltrasonography can be utilized to differentiate ICTH from FAVA, and the combined diagnosis method can further improve the technique’s diagnostic efficacy.
Machine learning extracts marks of thiamine’s role in cold acclimation in the transcriptome of Vitis vinifera
Authors: Konecny Tomas , Nikoghosyan Maria , Binder Hans 
Published: December 2023

IntroductionThe escalating challenge of climate change has underscored the critical need to understand cold defense mechanisms in cultivated grapevine Vitis vinifera. Temperature variations can affect the growth and overall health of vine.MethodsWe used Self Organizing Maps machine learning method to analyze gene expression data from leaves of five Vitis vinifera cultivars each treated by four different temperature conditions. The algorithm generated sample-specific “portraits” of the normalized gene expression data, revealing distinct patterns related to the temperature conditions applied.ResultsOur analysis unveiled a connection with vitamin B1 (thiamine) biosynthesis, suggesting a link between temperature regulation and thiamine metabolism, in agreement with thiamine related stress response established in Arabidopsis before. Furthermore, we found that epigenetic mechanisms play a crucial role in regulating the expression of stress-responsive genes at low temperatures in grapevines.DiscussionApplication of Self Organizing Maps portrayal to vine transcriptomics identified modules of coregulated genes triggered under cold stress. Our machine learning approach provides a promising option for transcriptomics studies in plants.
Integration of Shangshan culture into the STEAM curriculum and teaching: results of an interview-based study
Authors: Qian Chen , Ye Jian-Hong , Zheng Chaocan 
Published: December 2023

IntroductionInterdisciplinary science, technology, engineering, arts, and mathematics (STEAM) courses are a popular trend in international education than can help inculcate creativity in students. Although STEAM courses have been widely promoted in China, they are generally unsustainable because they are merely imitations of European and American courses and lack Chinese humanistic factors; a close integration between disciplinary ideas and thinking levels is also lacking. C-STEAM, which is designed to pass down China’s culture, is a form of STEAM education with local Chinese characteristics that are focused on integrating interdisciplinary knowledge with the thought process oriented toward cultural heritage.MethodsIn this study, an innovative higher vocational college course with C-STEAM interdisciplinary principles was constructed, with art and design as the framework, and with the integration of Chinese local culture. Semi-structured interviews were conducted to sample 12 learners from a total of 90 students in the experimental class of the C-STEAM course. The study aimed to provide a deeper understanding of the effectiveness of taking a Packaging Design course combined with C-STEAM from multiple perspectives. After the interviews, based on the BAO model, coding statistics and thematic analysis were conducted to understand the learners’ beliefs, actions, and outcomes after taking the course, and their plans for acquiring C-STEAM interdisciplinary knowledge and learning Chinese local culture.ResultsThe integration of the Shangshan culture (上山文化) into the Packaging Design course proved the importance and significance of adding C-STEAM to the art design course, which helped us understand the specific feelings of students after completing the course and gain a deeper understanding of the changes in their knowledge and skills and their learning effects.DiscussionIntegrating C-STEAM education into courses related to art and design is highly warranted to encourage students to apply their interdisciplinary knowledge to artistic exploration and creation. Moreover, to effectively develop a curriculum system with local characteristics, teachers should provide more opportunities for students to explore and learn C-STEAM in the future, and integrate multiple elements into their teaching. In general, a cultural perspective-based interdisciplinary education helps facilitate the creative transformation of traditional Chinese culture.
A single 1,500 m freestyle at maximal speed decreases cognitive function in athletes
Authors: Lai Zhijie , Huang Weiwei , Lin Wentao , Weng Xiquan , Mao Yuheng , Xu Guoqin 
Published: December 2023

IntroductionPhysical exercise can improve cognitive function, and the degree of impact on cognitive function is related to exercise modality, intensity, and duration. However, few studies have been conducted on the effects of competitive sports on cognitive function. The 1,500 m freestyle is the longest pool-based swimming event in the Olympic Games. This study explores the effects of 1,500 m freestyle at maximal speed on athletes’ cognitive function and analyzes the potential mechanism of cognitive function reduction in freestyle at maximal speed from the perspective of hemoglobin oxygenation difference (Hbdiff).MethodsA total of 13 male university swimmers were required to take part in a 1,500 m freestyle competition, swimming at maximal speed. The relevant indicators, including cognitive function and freestyle at maximal speed, before and after the competition were tested and analyzed. Cognitive function was assessed using the Schulte grid test (SGT), the trail-making test (TMT), and the digit span test (DST). The neurobiological characteristics of cognitive function, such as the prefrontal cortex (PFC), response time (RT), and accuracy rate (ACC), were tested using functional near-infrared spectroscopy (fNIRS).ResultsA significant decrease in scores for SGT, TMT, and digit span test-backward (DST-B) (p &lt; 0.01). Oxygenated hemoglobin (Oxy-Hb) concentrations in the right frontopolar area (R-FPA) of brain channels 8 (p &lt; 0.01) and 9 (CH8, 9) (p &lt; 0.05), the right dorsolateral prefrontal cortex (R-DLPFC) CH10 (p &lt; 0.05), and the middle dorsolateral prefrontal cortex (M-DLPFC) CH18 (p &lt; 0.01) were significantly altered, and the right area of the brain was activated. The total Oxy-Hb concentrations in the regions of interest (ROIs) of R-FPA, R-DLFPC, and M-DLFPC were changed significantly (p &lt; 0.01).DiscussionThe exhaustive performance of a 1,500 m freestyle event resulted in both physical fatigue and a decline in cognitive function. This decline may be attributed to the activation of specific regions of interest, namely the FPA, DLPFC, and M-DLPFC, within the prefrontal cortex (PFC), as well as alterations in functional connectivity.
“COAGULATION”: a mnemonic device for treating coagulation disorders following traumatic brain injury—a narrative-based method in the intensive care unit
Authors: Quintana-Diaz Manuel , Anania Pasquale , Juárez-Vela Raúl , Echaniz-Serrano Emmanuel , Tejada-Garrido Clara Isabel , Sanchez-Conde Pilar , Nanwani-Nanwani Kapil , Serrano-Lázaro Ainhoa , Marcos-Neira Pilar , Gero-Escapa María , García-Criado Jorge , Godoy Daniel Agustín 
Published: December 2023

IntroductionCoagulopathy associated with isolated traumatic brain injury (C-iTBI) is a frequent complication associated with poor outcomes, primarily due to its role in the development or progression of haemorrhagic brain lesions. The independent risk factors for its onset are age, severity of traumatic brain injury (TBI), volume of fluids administered during resuscitation, and pre-injury use of antithrombotic drugs. Although the pathophysiology of C-iTBI has not been fully elucidated, two distinct stages have been identified: an initial hypocoagulable phase that begins within the first 24 h, dominated by platelet dysfunction and hyperfibrinolysis, followed by a hypercoagulable state that generally starts 72 h after the trauma. The aim of this study was to design an acronym as a mnemonic device to provide clinicians with an auxiliary tool in the treatment of this complication.MethodsA narrative analysis was performed in which intensive care physicians were asked to list the key factors related to C-iTBI. The initial sample was comprised of 33 respondents. Respondents who were not physicians, not currently working in or with experience in coagulopathy were excluded. Interviews were conducted for a month until the sample was saturated. Each participant was asked a single question: Can you identify a factor associated with coagulopathy in patients with TBI? Factors identified by respondents were then submitted to a quality check based on published studies and proven evidence. Because all the factors identified had strong support in the literature, none was eliminated. An acronym was then developed to create the mnemonic device.Results and conclusionEleven factors were identified: cerebral computed tomography, oral anticoagulant &amp; antiplatelet use, arterial blood pressure (Hypotension), goal-directed haemostatic therapy, use fluids cautiously, low calcium levels, anaemia-transfusion, temperature, international normalised ratio (INR), oral antithrombotic reversal, normal acid–base status, forming the acronym “Coagulation.” This acronym is a simple mnemonic device, easy to apply for anyone facing the challenge of treating patients of moderate or severe TBI on a daily basis.
Self-Efficacy dengan Kepatuhan Mengonsumsi Tablet Fe pada Ibu Hamil Trimester III di RSUD Al-Ihsan Provinsi Jawa Barat
Authors: Fitri Riska De , Kusniasih Susi , Sakti Bani 
Published: December 2023

Tingkat kesadaran ibu hamil dalam mengonsumsi tablet Fe masih kurang optimal. Berkaitan dengan self-efficacy yaitu keyakinan tentang ibu hamil untuk dapat mengonsumsi tablet Fe. Kemungkinan ibu hamil mengalami anemia akan dipengaruhi oleh efikasi diri dan kepatuhan minum tablet Fe. Tujuan penelitian untuk mengetahui hubungan self-efficacy dengan kepatuhan mengonsumsi tablet-Fe pada ibu hamil trimester tiga. Sebuah studi observasional analitik dengan desain cross-sectional adalah metode penelitian ini. Populasi terjangkau penelitian ini adalah ibu hamil pada trimester ketiga yang telah menggunakan 90 tablet Fe pada Mei 2023, metode accidental sampling dengan sampel sebanyak 57 responden. Menggunakan kuesioner, wawancara digunakan untuk mengumpulkan data. Uji Chi-square digunakan untuk tujuan analisis data. Di RSUD-Al-Ihsan Provinsi Jawa Barat, penelitian ini menemukan adanya korelasi (p-value=0,037) antara efikasi diri ibu hamil dengan kesediaan ibu-hamil untuk minum tablet Fe. Ketidakpatuhan tablet Fe 3,733 kali lebih mungkin terjadi pada ibu hamil dengan efikasi diri rendah. Efikasi diri ditemukan sebagai faktor risiko ibu hamil trimester III di RSUD Al-Ihsan Provinsi Jawa Barat tidak mengkonsumsi tablet Fe. Kepatuhan harus ditekankan oleh penyedia layanan kesehatan, ibu hamil yang patuh harus dilibatkan sebagai panutan, dan keluarga melibatkan diri sebagai pendamping.
Renaissance de la Marine royale marocaine depuis l'indépendance jusqu'à nos jours
Authors: el Mouatani M’Barek 
Published: December 2023

The Rebirth of the Royal Moroccan Navy from indépendance to the present

Obliged to cease operating at the beginning of the XXth Century, Morocco's fighting fleet was reborn at independence under the name of the Royal Moroccan Navy. Its development has occurred in three phases :
The putting of the structures in place and creation of a first naval group.

Then the acquisition of modern naval combat, maritime surveillance and transport vessels consequent on the missions entailed in the recovery of the southern Saharan provinces - and also an effort to develop infrastructures and training establishments ;

Finally, from 2002 the creation of a naval aviation component.

Throughout these periods, the evolution of the Royal Moroccan Navy has been accompanied by active assistance from the French fleet under the aegis of military cooperation agreements.