bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy Feedback and Delay Adaptation Effects in Visual and Tactile Duration Reproduction
Authors: Chen Lingyue , Brunner Stephanie , Shi Zhuanghua 
Published: December 2023

AbstractBrief actions involve multiple temporal cues that may not always synchronize, and in basic action-effect relationships, the effect is often delayed. How the brain incorporates delays across modalities in a sensorimotor duration reproduction remains unclear. To investigate this, we conducted two experiments on duration reproduction with delayed sensory feedback. Participants reproduced durations in either visual (Experiment 1) or tactile (Experiment 2) modalities. In the adaptation phase, an action-induced effect, either visual or tactile stimulation, was delayed by 150 ms in one session and synchronized in the control session, with accuracy feedback provided after each trial. In the testing phase, random action-effect delays (0-150 ms) was introduced, and accuracy feedback was removed. The findings revealed that accuracy feedback effectively recalibrated motor time but did not eliminate the delay. Without accuracy feedback, tactile reproduction relied more on the tactile feedback than on motor time, resulting in greater lengthening of motor reproduction compared to the visual feedback. These findings suggest that temporal delay adaptation is influenced by accuracy feedback and sensorimotor integration, with sensorimotor reliability assigning a higher weight to the tactile than the visual modality.
The behavioural responses of bumblebeesBombus terrestrisin response to simulated rain
Authors: Reeves Laura A. , Jarvis Ellie M. , Lawson David A. , Rands Sean A. 
Published: December 2023

AbstractBumblebee activity typically decreases during rainfall, putting them under the threat of the increased frequency of precipitation due to climate change. A novel rain machine was used within a flight arena to observe the behavioural responses of bumblebees (Bombus terrestris) to simulated rain at both a colony and individual level. During rainfall, a greater proportion of workers left the arena than entered, the opposite of which was seen during dry periods, implying that they compensate for their lack of activity when conditions improve. The proportion of workers flying and foraging decreased while resting increased in rain. This pattern reversed during dry periods, providing further evidence for compensatory activity. The increase in resting behaviour during rain is thought to evade the high energetic costs of flying whilst wet without unnecessarily returning to the nest. This effect was not repeated in individual time budgets, measured with lone workers, suggesting that the presence of conspecifics accelerates the decision of their behavioural response, perhaps via local enhancement. Bumblebees likely use social cues to strategise their energetic expenditure during precipitation, allowing them to compensate for the reduced foraging activity during rainfall when conditions improve.
Coherent categorical information triggers integration-related alpha dynamics
Authors: Chen Lixiang , Cichy Radoslaw Martin , Kaiser Daniel 
Published: December 2023

AbstractTo create coherent visual experiences, the brain spatially integrates the complex and dynamic information it receives from the environment. We previously demonstrated that feedback-related alpha activity carries stimulus-specific information when two spatially and temporally coherent naturalistic inputs can be integrated into a unified percept. In this study, we sought to determine whether such integration-related alpha dynamics are triggered by categorical coherence in visual inputs. In an EEG experiment, we manipulated the degree of coherence by presenting pairs of videos from the same or different categories through two apertures in the left and right visual hemifields. Critically, video pairs could be video-level coherent (i.e., stem from the same video), coherent in their basic-level category, coherent in their superordinate category, or incoherent (i.e., stem from videos from two entirely different categories). We conducted multivariate classification analyses on rhythmic EEG responses to decode between the video stimuli in each condition. As the key result, we significantly decoded the video-level coherent and basic-level coherent stimuli, but not the superordinate coherent and incoherent stimuli, from cortical alpha rhythms. This suggests that alpha dynamics play a critical role in integrating information across space, and that cortical integration processes are flexible enough to accommodate information from different exemplars of the same basic-level category.
A Ratiometric Nonfluorescent CRISPR Assay Utilizing Cas12a-Induced Plasmid Supercoil Relaxation
Authors: Mohammad Noor , Talton Logan , Dalgan Selen , Wei Qingshan 
Published: December 2023

AbstractMost CRISPR-based biosensors rely on labeled reporter molecules and expensive equipment for signal readout. A recent alternative approach quantifies analyte concentration by sizing native λ DNA reporters, providing a simple and label-free solution for ultrasensitive detection. However, this method faces challenges in accurately quantifying size reduction of long DNA reporters via gel electrophoresis due to the sensitivity of DNA band shift to other interferences such as gel distortion. To overcome these limitations, here we developed a simple and robust ratiometric signaling strategy using CRISPR-Cas12a-induced supercoil relaxation of dsDNA plasmid reporters. In the presence of target, we observed that the fraction of supercoiled plasmid DNA decreased, and the amount of relaxed conformation (circular) increased over time. The relative percentage of supercoiled DNA to the relaxed circular DNA was analyzed by gel electrophoresis to generate an intensity-based ratiometric signal for more accurate target concentration quantification. This simple and inexpensive method is ∼100 times more sensitive when compared with the typical fluorescent reporter system. This self-referenced strategy solves the potential application limitations of previously demonstrated DNA sizing-based CRISPR-Dx without compromising the sensitivity. Finally, we demonstrated the applicability of ratiometric sensing strategy using model DNA targets such as AAV and HPV 16, highlighting its feasibility for point-of-use CRISPR-Dx applications.Table of Contents (TOC):
Association of alcohol consumption with circulating biomarkers of atrial fibrillation-related pathways in a population at high cardiometabolic risk
Authors: Alam Aniqa B. , Toledo-Atucha Estefania , Romaguera Dora , Alonso-Gómez Angel M. , Martínez-Gonzalez Miguel A. , Tojal-Sierra Lucas , Razquin Cristina , Noris Mora Marta , Li Linzi , Subramanya Vinita , Salas-Salvadó Jordi , Fitó Montserrat , Alonso Alvaro 
Published: December 2023

ABSTRACTBackgroundThe effect of alcohol consumption on cardiovascular health, including atrial fibrillation risk, remains controversial. Evaluating the association of alcohol consumption with circulating atrial fibrillation-related biomarkers may help better understand the relevant mechanistic underpinnings.MethodsWe studied 523 participants from 3 sites for the PREDIMED-Plus study, a weight-loss randomized intervention trial in metabolically unhealthy adults. N-terminal pro-B-type natriuretic protein (NTproBNP), high sensitivity troponin-T (hsTnT), high-sensitivity C-reactive protein (hsCRP), 3-nitrotyrosine (3-NT), and procollagen type 1 carboxy-terminal propeptide (PICP) were measured in fasting serum samples at baseline and years 3 and 5 of follow-up. We calculated alcohol consumption in drinks/day (1 drink = 14 grams alcohol) with validated food frequency questionnaires at each visit. Using multiple linear regression and mixed models we estimated the association of alcohol consumption with log-transformed biomarkers at baseline and longitudinally adjusting for potential confounders.ResultsAmong 523 participants (mean age: 65 years, 40% female), mean alcohol consumption was 1 drink/day. Cross-sectionally, alcohol consumption was not associated with cardiac biomarker concentrations. Longitudinally, compared to non-consumers, heavy drinkers (≥4 drinks/day) had smaller increases in hsTnT (β: -0.11, 95%CI: -0.20, -0.01)and PICP (β: -0.15, 95%CI: -0.30, 0.01) over the 5-year follow-up. In contrast, those who increased alcohol consumption over the 5-year period experienced greater increases in hsCRP (β: 0.42, 95%CI: 0.11, 0.73) compared to those whose drinking behavior stayed the same.ConclusionAlcohol consumption was associated with complex changes in circulating biomarkers, including comparatively lower fibrotic and myocardial damage, but higher levels of overall inflammation over time. These results underscore the need for further research to better understand the effects of alcohol on cardiovascular health.
Dimerization of ADAR1 modulates site-specificity of RNA editing
Authors: Mboukou Allegra , Rajendra Vinod , Messmer Serafina , Catala Marjorie , Tisné Carine , Jantsch Michael F. , Barraud Pierre 
Published: December 2023

AbstractAdenosine-to-inosine editing is catalyzed by adenosine deaminases acting on RNA (ADARs) in double-stranded RNA (dsRNA) regions. Although three ADARs exist in mammals, ADAR1 is responsible for the vast majority of the editing events and acts on thousands of sites in the human transcriptome. ADAR1 has been proposed to form a stable homodimer and dimerization is suggested to be important for editing activity. In the absence of a structural basis for the dimerization of ADAR1, and without a way to prevent dimer formation, the effect of dimerization on enzyme activity or site specificity has remained elusive. Here, we report on the structural analysis of the third double-stranded RNA-binding domain of ADAR1 (dsRBD3), which reveals stable dimer formation through a large inter-domain interface. Exploiting these structural insights, we engineered an interface-mutant disrupting ADAR1-dsRBD3 dimerization. Notably, dimerization disruption did not abrogate ADAR1 editing activity but intricately affected editing efficiency at selected sites. This suggests a complex role for dimerization in the selection of editing sites by ADARs, and makes dimerization a potential target for modulating ADAR1 editing activity.
Conserved autism-associated genes tune social feeding behavior inC. elegans
Authors: Cowen Mara H. , Reddy Kirthi C. , Chalasani Seekanth H. , Hart Michael P. 
Published: December 2023

ABSTRACTAnimal foraging is an essential and evolutionarily conserved behavior that occurs in social and solitary contexts, but the underlying molecular pathways are not well defined. We discover that conserved autism-associated genes (NRXN1(nrx-1),NLGN3(nlg-1),GRIA1,2,3(glr-1),GRIA2(glr-2), andGLRA2,GABRA3(avr-15))regulate aggregate feeding inC. elegans, a simple social behavior. NRX-1 functions in chemosensory neurons (ADL and ASH) independently of its postsynaptic partner NLG-1 to regulate social feeding. Glutamate from these neurons is also crucial for aggregate feeding, acting independently of NRX-1 and NLG-1. Compared to solitary counterparts, social animals show faster presynaptic release and more presynaptic release sites in ASH neurons, with only the latter requiringnrx-1. Disruption of these distinct signaling components additively converts behavior from social to solitary. Aggregation induced by circuit activation is also dependent onnrx-1. Collectively, we find that aggregate feeding is tuned by conserved autism-associated genes through complementary synaptic mechanisms, revealing molecular principles driving social feeding.TEASERConserved autism-associated genes mediate distinct molecular and circuit signaling components that cooperate to tuneC. eleganssocial feeding behavior.
Allelic gene conversion softens selective sweeps
Authors: Schrider Daniel R. 
Published: December 2023

AbstractThe prominence of positive selection, in which beneficial mutations are favored by natural selection and rapidly increase in frequency, is a subject of intense debate. Positive selection can result in selective sweeps, in which the haplotype(s) bearing the adaptive allele “sweep” through the population, thereby removing much of the genetic diversity from the region surrounding the target of selection. Two models of selective sweeps have been proposed: classical sweeps, or “hard sweeps”, in which a single copy of the adaptive allele sweeps to fixation, and “soft sweeps”, in which multiple distinct copies of the adaptive allele leave descendants after the sweep. Soft sweeps can be the outcome of recurrent mutation to the adaptive allele, or the presence of standing genetic variation consisting of multiple copies of the adaptive allele prior to the onset of selection. Importantly, soft sweeps will be common when populations can rapidly adapt to novel selective pressures, either because of a high mutation rate or because adaptive alleles are already present. The prevalence of soft sweeps is especially controversial, and it has been noted that selection on standing variation or recurrent mutations may not always produce soft sweeps. Here, we show that the inverse is true: selection on single-originde novomutations may often result in an outcome that is indistinguishable from a soft sweep. This is made possible by allelic gene conversion, which “softens” hard sweeps by copying the adaptive allele onto multiple genetic backgrounds, a process we refer to as a “pseudo-soft” sweep. We carried out a simulation study examining the impact of gene conversion on sweeps from a singlede novovariant in models of human,Drosophila, andArabidopsispopulations. The fraction of simulations in which gene conversion had produced multiple haplotypes with the adaptive allele upon fixation was appreciable. Indeed, under realistic demographic histories and gene conversion rates, even if selection always acts on a single-origin mutation, sweeps involving multiple haplotypes are more likely than hard sweeps in large populations, especially when selection is not extremely strong. Thus, even when the mutation rate is low or there is no standing variation, hard sweeps are expected to be the exception rather than the rule in large populations. These results also imply that the presence of signatures of soft sweeps does not necessarily mean that adaptation has been especially rapid or is not mutation limited.
β-H-Spectrin is a key component of an apical-medial hub of proteins during cell wedging in tube morphogenesis
Authors: Gillard Ghislain , Röper Katja 
Published: December 2023

AbstractCoordinated cell shape changes are a major driver of tissue morphogenesis during development, with apical constriction or wedging of groups of epithelial cells for instance leading to tissue bending in folding or budding processes. During the budding of the tubes of the salivary glands in theDrosophilaembryo we previously identified a key interplay between the apical-medial actomyosin that drives apical constriction with the underlying longitudinal microtubule array. At this microtubule-actomyosin interface a hub of proteins accumulates: in addition to the microtubule-actin crosslinker Shot and the minus-end-binder Patronin, we identified two actin-crosslinkers, β-H-Spectrin and Filamin, and the multi-PDZ protein Big bang as components of this apical-medial hub. Tissue-specific degradation of β-H-Spectrin led to reduction of apical-medial Big bang, F-actin, Shot and Patronin and concomittant defects in apical constriction and tube morphogenesis. Residual Patronin still present in the apical-medial position was sufficient to assist microtubule reorganisation into the longitudinal array. In contrast to Patronin and Shot, neither β-H-Spectrin nor Big bang required microtubules for their localisation. β-H-Spectrin instead appeared to be recruited to the apical-medial domain via binding to phosphoinositides that accumulated here. Overexpression of a β-H-Spectrin fragment containing its PH domain displaced endogenous β-H-Spectrin from the apical-medial domain and led to strong morphogenetic defects. The interconnected hub therefore required the synergy of membrane-associated β-H-Spectrin and microtubules and their respective interactors for its assembly and function in sustaining the apical constriction during tube invagination.
HD-Zip II transcription factors control distal stem cell fate in Arabidopsis roots by linking auxin signaling to the FEZ/SOMBRERO pathway
Authors: Possenti Marco , Sessa Giovanna , Alfè Altea , Turchi Luana , Ruzza Valentino , Sassi Massimiliano , Morelli Giorgio , Ruberti Ida 
Published: December 2023

AbstractIn multicellular organisms, specialized tissues are generated by specific populations of stem cells through cycles of asymmetric cell divisions, where one daughter undergoes differentiation and the other maintains proliferative properties. InArabidopsis thalianaroots, the columella - a gravity-sensing tissue which protects and defines the position of the stem cell niche - represents a typical example of a tissue whose organization is exclusively determined by the balance between proliferation and differentiation. The columella derives from a single layer of stem cells through a binary cell fate switch that is precisely controlled by multiple, independent regulatory inputs. Here, we show that the HD-Zip II transcription factors HAT3, ATHB4, and AHTB2, redundantly regulate columella stem cell fate and patterning in the Arabidopsis root. The HD-Zip II transcription factors promote columella stem cell proliferation by acting as effectors of the FEZ/SMB circuit and, at the same time, by interfering with auxin signaling to counteract hormone-induced differentiation. Overall, our work shows that HD-Zip II connect two opposing parallel inputs to fine tune the balance between proliferation and differentiation in columella stem cells.Summary statementHD-Zip II transcription factors redundantly regulate columella stem cells activity by counteracting auxin-mediated differentiation downstream of the FEZ/SMB proliferative input
Investigating and modeling the circular economy enablers in product development process: net zero as a moderator
Authors: Sabale Dattatraya Balasaheb , Kaswan Mahender Singh , Rathi Rajeev , Yadav Vishwas 
Published: December 2023

PurposeIn the current era, the focus on circular economy (CE) has gained substantial momentum among the research communities across the manufacturing world. It has become the need of the hour to act fast due to the alarming issues of unsustainability such as climate change, global warming, waste generation, environmental pollution, resource scarceness and ecological degradations. This research aims to investigate and model the CE enablers in the product development process related to the moderating effect of net zero.Design/methodology/approachThe significant CE enablers are identified through literature review and expert brainstorming. The Pythagorean fuzzy decision-making trial and evaluation laboratory (PF-DEMATEL) technique has been used to investigate and evaluate the significant CE enablers in product development process. PF-DEMATEL determines the inter-relationship and casual dependency among the selected CE enablers. Indian automobile small and medium enterprises (SMEs) have been considered as a case organization to demonstrate the effectiveness of the proposed method.FindingsThe findings reveal that “Top management support and clear vision towards CE adoption” is the most important enabler and “Artificial intelligence in product value chain” is recognized as the least vital enabler. This research aids the managers, decision-makers, policy planners and workforce to develop and formulating efficient blueprints for the effective adoption of CE in Indian SMEs.Originality/valueThis is the first kind of research that explores CE enablers in product development process for Indian SMEs.
Antecedents of Industry 4.0 capabilities and technological innovation: a dynamic capabilities perspective
Authors: AL-Khatib Ayman wael , Shuhaiber Ahmed , Mashal Ibrahim , Al-Okaily Manaf 
Published: December 2023

Purpose
This paper aims to empirically examine the impact of dynamic capabilities on Industry 4.0 capabilities in small and medium enterprises (SMEs) in Jordan. It also aims to examine the potential impact of industry 4.0 capabilities on technological innovation.

Design/methodology/approach
Data were collected from 210 respondents who work and own SMEs in Jordan. SmartPLS software based on the partial least squares-structural equation modeling approach was used to test hypotheses.

Findings
The findings reveal the positive effects of the three components of dynamic capabilities, including sensing, seizing and reconfiguring, on Industry 4.0 capabilities. They also confirm the positive effect of Industry 4.0 capabilities on technological innovation.

Originality/value
This study provides valuable practical implications and enriches the literature on the determinants of Industry 4.0 capabilities and its role in developing the dynamic capabilities of SMEs, such as technological innovation.
Café Galavis: the challenge of internationalization of a family business
Authors: Perez Perez Juan Ernesto 
Published: December 2023

Learning outcomes
Upon completion of this case study, students will be able to identify the main conceptual foundations of strategy in international business, determine the strategy of entry into a country through the analysis of dual pressures and propose the mode of entry into a country by analyzing the sources of competitive advantage in a firm’s value chain.

Case overview/synopsis
Café Galavis stood out as one of the most outstanding family businesses in industrial development, with a century-long trajectory in the production and sale of roasted and ground coffee in Cucuta, Colombia. However, in 2015, the diplomatic, humanitarian and economic crisis between the governments of Colombia and Venezuela led to the indefinite closure of the Colombian–Venezuelan border. As a result, the illegal coffee trade increased, and competition from traditional brands significantly affected sales, leading to financial difficulties and an urgent need for change in senior management. In addition, the demise of the manager who had been leading the company until 2018 exacerbated the business situation. Juan Francisco Yáñez, the new manager, joined the management of an emblematic company. From his first years of management, he implemented different strategies related to organizational policies, production, innovation and export of products to achieve stability. For the year 2023, the challenges for the new CEO were to lead a brand with more than a century of tradition and business roots and to enter new markets, owing to the high dependence on the Venezuelan market. Which countries should they enter in the internationalization process? Furthermore, what type of strategy and entry mode should Café Galavis implement to penetrate new markets? These were some of the challenges faced by the CEO; therefore, he required objective information to make decisions in consensus with his collaborators.

Complexity academic level
This case study is suitable for students of postgraduate academic programs in knowledge areas of international management, international business or MBA.

Supplementary material
Teaching notes are available for educators only.

Subject code
CSS 5: International business.