bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infants who develop autism show smaller inventories of deictic and symbolic gestures at 12 months of age
Authors: Wu Dennis , Wolff Jason J. , Ravi Shruthi , Elison Jed T. , Estes Annette , Paterson Sarah , St. John Tanya , Abdi Hervé , Moraglia Luke E. , Piven Joseph , Swanson Meghan R. ,   for the IBIS Network
Published: January 2024

AbstractGestures are an important social communication skill that infants and toddlers use to convey their thoughts, ideas, and intentions. Research suggests that early gesture use has important downstream impacts on developmental processes, such as language learning. However, autistic children are more likely to have challenges in their gestural development. The current study expands upon previous literature on the differences in gesture use between young autistic and non‐autistic toddlers by collecting data using a parent‐report questionnaire called the MCDI–Words and Gestures at three time points, 12, 18, and 24 months of age. Results (N = 467) showed that high‐likelihood infants who later met diagnostic criteria for ASD (n = 73 HL‐ASD) have attenuated gesture growth from 12 to 24 months for both deictic gestures and symbolic gestures when compared to high‐likelihood infants who later did not meet criteria for ASD (n = 249 HL‐Neg) and low‐likelihood infants who did not meet criteria for ASD (n = 145 LL‐Neg). Other social communicative skills, like play behaviors and imitation, were also found to be impacted in young autistic children when compared to their non‐autistic peers. Understanding early differences in social communication growth before a formal autism diagnosis can provide important insights for early intervention.
Control of phytopathogen organisms using bioactive compounds contained in orange wax
Authors: García‐Cruz Ariel , Díaz‐Jiménez Lourdes , Enríquez‐Vara Jhony , Mtz‐Enríquez Arturo I. , Quiñones‐Aguilar Evangelina E. , Ramos‐González Rodolfo , Aguilar‐González Cristóbal N. , Martínez‐Hernández José L. , Rincón‐Enríquez Gabriel , Iliná Anna 
Published: January 2024

AbstractOrange wax (OW) is a waxy waste generated in the essential oil purification process (winterization). It has no commercial value. It is discarded or burnt, polluting the environment. The present study demonstrates the possibility of applying it to control phytopathogens. The ethanol‐soluble bioactive compounds of OW were identified and quantified. The effect of orange wax extracts on the growth inhibition of phytopathogenic fungi (Fusarium oxysporum and Colletotrichum gloeosporioides) and Spodoptera frugiperda larvae was evaluated. High‐performance liquid chromatography–mass spectrometry (HPLC‐MS) and gas chromatography–mass spectrometry (GC–MS) quantified 22 bioactive compounds representing 23% (w/w) of OW. Terpineol, limonene, and eremophilene were the most abundant bioactive substances in this material. The distortion of the radial growth of the fungal hyphae was measured in a potato dextrose agar (PDA), and all tested concentrations (6.3, 12.5, 25, 50, and 100 mg/mL) inhibited the radial expansion of C. gloeosporioides. In contrast, only the OW extracts at 50 and 100 mg mL−1 inhibited F. oxysporum. Spray treatments were performed using a Potter spray tower on third‐instar Spodoptera frugiperda larvae. Spraying OW extract at 50 mg mL−1 for 10 s caused the highest larval mortality. However, third‐instar larvae were attracted to consume corn leaves moistened with extract at lower concentrations. Up to 93% of the first‐instar larvae died after eating corn leaves moistened with the extract at 50 mg mL−1. As far as the authors are aware, this is the first scientific report about the use of bioactive compounds of orange wax to control fungi. Spodoptera frugiperda could form the basis for the manufacture of new biopesticides.
Testosterone nadir and clinical outcomes in patients with advanced prostate cancer: Post hoc analysis of triptorelin pamoate Phase III studies
Authors: Klotz Laurence , Tat Tri 
Published: January 2024

AbstractObjectiveThe objective of the study is to evaluate whether low nadir testosterone during treatment with triptorelin pamoate, a luteinising hormone‐releasing hormone (LHRH) agonist, is associated with improved clinical outcomes in patients with advanced prostate cancer using a retrospective analysis of clinical trial data.Patients and methodsData were pooled from three prospective, 9–12‐month Phase III studies of triptorelin monotherapy in patients with advanced prostate cancer (including NCT00751790). The serum testosterone concentration suppression targets evaluated were <0.35 nmol/L (<10 ng/dl), <0.7 nmol/L (<20 ng/dl), <1.7 nmol/L (<50 ng/dl) and ≥1.7 nmol/L. Overall survival (OS) and disease‐specific survival (DSS) by testosterone suppression group were assessed by Kaplan–Meier analysis, with log‐rank test. The time frame for the primary analysis was Days 1–518 (median OS follow‐up 254 days [range, 29–518 days]) and for the sensitivity analyses was Days 1–262. Supplementary analyses combined the ≥0.7‐ to <1.7‐nmol/L and ≥1.7‐nmol/L groups.ResultsThe sample size comprised 592 patients (most received triptorelin monotherapy; four reported concomitant androgen receptor‐axis–targeted therapy). Nadir testosterones of <0.35, ≥0.35 to <0.7, ≥0.7 to <1.7 and ≥1.7 nmol/L were achieved by 96%, 3.2%, 0.34% and 0.17% of patients, respectively. Better OS with decreasing level of nadir testosterone was observed (p < 0.001) and this persisted after sensitivity/supplemental analyses (all p < 0.001). Differences in DSS with decreasing levels of nadir testosterone were not statistically significant in the primary analysis. Sensitivity/supplemental analysis showed better DSS with decreasing level of nadir testosterone (Days 1–262, p = 0.01; combined groups Days 1–518, p = 0.03; combined groups Days 1–262, p = 0.005).ConclusionLow nadir testosterone achieved during treatment with the LHRH agonist triptorelin was associated with improved OS and DSS in patients with advanced prostate cancer.
Patient perspectives of weight stigma across the cancer continuum: A scoping review
Authors: Hawley Nanako , Green Jennifer , Ahlich Erica , Hauff Caitlyn , Hermer Janice , Skiba Meghan B. , James Dara L. , Nash Sarah H. 
Published: January 2024

AbstractBackgroundWeight stigma has been defined as the social devaluation and denigration of individuals because of their weight. The purpose of this scoping systematic review was to assess and understand patient experiences with weight stigma in the cancer care setting.MethodsWe conducted a systematic scoping review of studies examining shame, prejudice, bias, and stigma in relation to weight and cancer‐related care using five databases: PubMed, CINAHL Plus Full Text (ProQuest), Cochrane Library, PsycINFO (EBSCO), and Scopus. Articles were uploaded into Covidence for de‐duplication and screening. Included studies were peer reviewed, reported adult patient experiences in cancer‐related care, and were published in English between October 2012 and February 2023. Study characteristics and key findings were abstracted and qualitatively synthesized.ResultsPublications meeting inclusion criteria yielded five studies (n = 113 participants). Most focused on the experiences of women (n = 4) and cancers which predominantly impact women (i.e., breast, cervical, endometrial; n = 4). All stages of the cancer continuum were included with studies examining screening (n = 2), treatment (n = 1), and post‐treatment survivorship (n = 2). Weight discrimination was discussed in four studies and weight‐biased stereotypes were discussed in three studies. Experiences of weight bias internalization were reported in four studies. One study described an instance of implicit weight bias.ConclusionsLimited studies examine patient experiences of weight stigma in cancer care; however, current evidence suggests that patients do experience weight stigma in cancer‐related care. This review highlights critical gaps and a need for more research on the prevalence and impact of weight stigma in cancer screening and care.
Oral manifestation as the only sign of Langerhans cell histiocytosis: A case report
Authors: Nazemisalman Bahareh , Zarabadi Mobina Sadat 
Published: January 2024

Key Clinical MessageThe manifestations of Langerhans cell histiocytosis can be limited in the oral cavity, including gingival recession, tooth mobility, and bone destruction. Dentists play a crucial role in the early detection and management of these oral symptoms, which can significantly impact the overall prognosis and quality of life for patients with this serious disease.AbstractThe hyperplastic activity of bone marrow can lead to excessive histocyte proliferation, called Langerhans cell histiocytosis (LCH). Based on the accumulation location, it has various organ involvement, including skin, bone, and so forth. Oral manifestations such as tooth involvement, bone lesions, mucosal ulcers, and periodontal problems can occur, and they might be the only manifestation of LCH. A subtle diagnosis is crucial and can be started with dental examinations. A 5‐year‐old girl was examined with the chief complaint of gingival recession with no pain, caries, or calculus. The clinical and radiographical assessment led to further immunohistochemical (IHC) evaluations. The diagnosis was LCH based on S‐100 and cluster of differentiation 1a (CD1a) markers. LCH can involve different cells and tissues locally or generally. The early detection and treatment of LCH increase children's survival rate and quality of future life. Therefore, an accurate diagnosis is important among dentists. They must consider that abnormal mobility, gingival, or periodontal problems with no logical cause might warn about a latent problem. Sometimes extraction of loose teeth is not the end of treatment; it is the start of neglecting a serious disease.
First‐Row Transition‐Metal Complexes with Tetra‐Amido Macrocyclic Ligands for Water and C(sp3)−H Bond Oxidation: Performance Benchmarking Using Free Energy Relationships
Authors: Lin Chien‐Wen , Chuang Yu‐Wei , Lu Kuan‐Yu , Wang Yu‐Heng 
Published: January 2024

AbstractThe catalytic oxidation of water and C(sp3)−H bonds enables the conversion of solar/electrochemical energy into chemical energy and alcohol fuel production, respectively, and therefore plays important roles in energy conversion/storage. These transformations are typically catalyzed by the highly active complexes of the earth‐abundant first‐row transition metals (Mn, Fe, Co, Ni, and Cu) with tetra‐amido macrocyclic ligands (TAMLs), although the related kinetics and thermodynamics remain underexplored. This review uses free energy relationships between kinetic and thermodynamic descriptors as normalized metrics to benchmark/compare these TAML‐based catalysts and assess the advances in their development.
An Ionic Liquid‐Based Gel Electrolyte: Formation Mechanism and Feasibility for Lithium Metal Batteries
Authors: Gu Yu , Chen Hao‐Ning , Wang Wei‐Wei , Yan Hao , Yan Jia‐Wei , Mao Bing‐Wei 
Published: January 2024

AbstractQuasi‐solid‐state electrolytes (QSSEs) based on ionic liquids are recognized as one of the frontrunners of electrolytes for ensuring the safety and high energy density of next‐generation lithium batteries. However, the incapacity of such systems to meet the performance demands of batteries is significantly subject to the properties of the electrolyte and the intricacies of related interfacial processes. Recently, we have designed a high‐performing pyrrolidinium bis(trifluoromethylsulfonyl)imide (Py14TFSI) based QSSE (Py‐Gel), with introducing LiBF4 and LiCl, which served as a system for probing the dynamic Li deposition mechanism. Herein, we focus on understanding the mechanisms of Py‐Gel formation and examining the chemistry and structure as well as the properties of the solid‐electrolyte interphases (SEIs) formed in the Py‐Gel and their influences on Li deposition. Spectroscopic characterizations suggest the existence of a cross‐linked structure in Py‐Gel, for which multiple intermolecular/intramolecular hydrogen bondings and ionic coulumbic interactions among Py cations, TFSI− anions, and Li salts are responsible. The Py‐Gel electrolyte exhibits exceptional electrical properties at room temperature. Different SEIs are prepared in the Py‐Gel via electrochemical protocols to elucidate that the synergy between a well‐featured electrolyte and an outstanding SEI is vital for stable cycling of Li metal anodes in such a QSSE.
The difference of model robustness assessment using cross‐validation and bootstrap methods
Authors: Lasfar Rita , Tóth Gergely 
Published: January 2024

AbstractThe validation principles on Quantitative Structure Activity Relationship issued by Organization for Economic and Co‐operation and Development describe three criteria of model assessment: goodness of fit, robustness and prediction. In the case of robustness, two ways are possible as internal validation: bootstrap and cross‐validation. We compared these validation metrics by checking their sample size dependence, rank correlations to other metrics and uncertainty. We used modeling methods from multivariate linear regression to artificial neural network on 14 open access datasets. We found that the metrics provide similar sample size dependence and correlation to other validation parameters. The individual uncertainty originating from the calculation recipes of the metrics is much smaller for both ways than the part caused by the selection of the training set or the training/test split. We concluded that the metrics of the two techniques are interchangeable, but the interpretation of cross‐validation parameters is easier according to their similar range to goodness‐of‐fit and prediction metrics. Furthermore, the variance originating from the random elements of the calculation of cross‐validation metrics is slightly smaller than those of bootstrap ones, if equal calculation load is applied.
Self‐immolation Assisted Morphology Transformation of Prebiotic Lipidated‐cationic Amino Acids: Electro‐droplet Mediated C−C Coupling Reaction to Synthesize Macromolecules
Authors: Hazra Bibhas , Mandal Raki , Sahu Jayati , Das Subrata , Prasad Mahesh , Tarafdar Pradip K. 
Published: January 2024

AbstractCompartmentalization protected biomolecules from the fluctuating environments of early Earth. Although contemporary cells mostly use phospholipid‐based bilayer membranes, the utility of non‐bilayer compartments was not ruled out during the prebiotic and modern eras. In the present study, we demonstrated the prebiotic synthesis of lipidated cationic amino acid‐based amphiphiles [lauryl ester of lysine (LysL); ornithine (OrnL); and 2,4‐diamino butyric acid (DabL)] using model dry‐down reaction. These amphiphiles self‐assemble into micellar membranes. However, the OrnL and DabL‐based micelles undergo pH‐responsive transformation to lipid droplet‐like morphologies, a modelcompartment in the prebiotic Earth. These cationic droplets encapsulated prebiotic molecules (isoprene) and assisted electron transfer reaction to synthesize isoprenoid derivatives at primitive Earth conditions. The self‐assembly of prebiotic amphiphiles, their transformation to droplet compartments, and droplet‐assisted C−C bond formation reaction might have helped the evolution to synthesize various biomolecules required for the origin of life.
Aptamer Clicked Poly(ferrocenylsilanes) at Au Nanoparticles as Platforms with Multiple Function[†]
Authors: Halmagyi Tibor G. , Alsharif Nizar B. , Berkal Mohamed A. , Hempenius Mark A. , Szilagyi Istvan , Vancso G. Julius , Nardin Corinne 
Published: January 2024

AbstractAptamers are widely used in biosensing due to their specific sensitivity toward many targets. Thus, gold nanoparticle (AuNP) aptasensors are subject to intense research due to the complementary properties of aptamers as sensing elements and AuNPs as transducers. We present herein a novel method for the functional coupling of thrombin‐specific aptamers to AuNPs via an anionic, redox‐active poly(ferrocenylsilane) (PFS) polyelectroyte. The polymer acts as a co‐reductant and stabilizer for the AuNPs, provides grafting sites for the aptamer, and can be used as a redox sensing element, making the aptamer‐PFS‐AuNP composite (aptamer‐AuNP) a promising model system for future multifunctional sensors. The aptamer‐AuNPs exhibit excellent colloidal stability in high ionic strength environments owing to the combined electrosteric stabilizing effects of the aptamer and the PFS. The synthesis of each assembly element is described, and the colloidal stability and redox responsiveness are studied. As an example to illustrate applications, we present results for thrombin sensitivity and specificity using the specific aptamer.
Continuous Direct Mechanocatalytic Suzuki‐Miyaura Coupling via Twin‐Screw Extrusion
Authors: Chantrain Viviene , Rensch Tilo , Pickhardt Wilm , Grätz Sven , Borchardt Lars 
Published: January 2024

AbstractThis work establishes the first direct mechanocatalytic reaction protocol within an extruder, focusing on the Suzuki‐Miyaura reaction. Through the coating of either the extruder screws or barrel with Pd, we executed the cross‐coupling reaction without the reliance on molecular catalyst compounds or powders, and solvents continuously. We identified the influence and interplay of crucial reaction parameters such as temperature, mechanical energy input, residence time, rheology, and catalyst contact time and finally obtained 36 % and 75 % of the reaction product after one and four reactor passes respectively.
Tumor mutation load better predicts the prognosis of patients treated with immune checkpoint inhibitors in upper gastrointestinal cancers: A systematic review and meta‐analysis
Authors: Ma Chenghao , Teng Qiong , Shang Liang , Du Fengying , Li Leping 
Published: January 2024

AbstractBackgroundTumor mutational load (TML) has emerged as a potential biomarker for multiple solid tumors. However, data on its prognostic impact on upper gastrointestinal (UGI) cancer are limited. Therefore, the aim of this systematic review and meta‐analysis was to assess the prognostic value of TML for the survival of patients with UGI cancer.MethodA comprehensive search of the PubMed, Embase, Cochrane Library, and Web of Science databases was conducted up to February 13, 2023. Eleven studies met our inclusion criteria. Hazard ratios (HRs) for progression‐free survival and overall survival and their 95% confidence intervals (CIs) were calculated. Subsequently, the combined HR and its 95% CI were calculated for UGI tract cancers in the high and low TML groups. I2 statistics and p‐values were used to evaluate heterogeneity. Publication bias, sensitivity, and subgroup analyses were performed to determine sources of heterogeneity.ResultsIn total, 932 patients with UGI tract cancer from 11 publications were included. The high TML group treated with immunotherapy showed significantly improved overall survival (HR = 0.68; 95% CI: 0.53, 0.86; p = .001) and progression‐free survival (HR = 0.74; 95% CI: 0.58, 0.95; p = .020) compared with the low TML group.ConclusionOur study demonstrated that patients with UGI tumors and higher TML have a better prognosis with immunotherapy, suggesting that TML is a promising predictive biomarker for immunotherapy.RegistrationThe study protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO Registration No: CRD42023405596).
Preparation of biochars by conventional pyrolysis of herbal waste and their potential application for adsorption and energy purposes
Authors: Gęca Marlena , Wiśniewska Małgorzata , Nowicki Piotr 
Published: January 2024

AbstractThe nettle, sage, mint and lemon balm herbs were used for biochars preparation. The physicochemical parameters of obtained materials were related to the lignocellulose composition of the precursors. It has been proved that the content of mineral substance has a significant influence on development of surface area, whereas the amount of hemicellulose affects the content of surface functional groups. It has been also shown that the obtained biochars are characterized by great energy parameters. The higher heating values (HHV) of the carbonaceous materials are comparable to the typical energy sources. The greatest HHV value (20.36 MJ/kg) was characteristic for the biochar obtained by pyrolysis of the lemon balm. In addition, the biochars were used for ionic polymers adsorption from one‐ and two‐components solutions. Despite the adsorbed amounts of macromolecules are not great is has been proved that polyethylenimine and polyacrylic acid have positive influence on their mutual adsorption.
Effect of NO2 on N2O production and NOx emission reduction in NH3 Selective Catalytic Reduction
Authors: Lv Heyin , Li Shilong , Yang Miansong , Liu Mingshun , Li Zhijun 
Published: January 2024

AbstractWith the introduction of increasingly strict emission regulations, reducing nitrogen oxide (NOx) emissions and nitrous oxide (N2O) production from diesel engines have become the focus of research. At high temperature, the reaction of NO2 in the catalyst generates the intermediate product NH4NO3, which first crystallizes below 300 °C. These crystals tend to block the pores and inhibit the reaction. Subsequently, N2O is produced through the decomposition of NH4NO3, leading to additional pollution. Therefore, the concentration of NO2 has a direct impact on both the NOx conversion efficiency and the generation of N2O, requiring consideration of the optimal proportion of NO2 in SCR. Considering these two factors, it is concluded that the optimal amount of NO2 varies with temperature. To improve the NOx conversion rate of the Cu‐SSZ‐13 catalyst at low temperatures and reduce N2O generation, the optimal NO2 ratio of the Cu‐SSZ‐13 catalyst under various operating conditions is studied using numerical simulations. As the temperature rises, the optimal NO2/NOx ratio first increases and then decreases. Under the optimal NO2/NOx ratio, the NOx conversion rate significantly increases, while N2O generation decreases considerably. The optimal NO2/NOx ratio also provides suggestions for the optimization of the DOC‐DPF‐DCR system.
Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library
Authors: Morselli Marco , Holton Thomas R. , Pellegrini Matteo , Yeates Todd O. , Arbing Mark A. 
Published: January 2024

AbstractProtein display systems are powerful techniques used to identify protein molecules that bind with high affinity to target proteins of interest. The initial challenge in implementing a display system is the construction of a high‐diversity naïve library. Here, we describe the methods to generate a designed ankyrin repeat protein (DARPin) display library using degenerate oligonucleotides. Specifically described is the construction of a single DARPin repeat module by overlap extension PCR, concatenation of the module by restriction enzyme digestion and ligation, and incorporation of the concatenated modules into a full‐length DARPin sequence in a bacterial cloning or display vector containing the hydrophilic N‐ and C‐terminal capping domains. Protocols for PCR amplification of DARPin sequences to estimate diversity of naïve and enriched libraries via next‐generation sequencing are included, as is a simple Linux‐based program for analysis of naïve and enriched sequences. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Generation of a single DARPin repeat by overlap extension PCRBasic Protocol 2: Concatenation of DARPin repeatsBasic Protocol 3: Ligation of internal repeats into cloning/display vector containing N‐ and C‐terminal capping repeatsBasic Protocol 4: Estimation of library size and diversity by next‐generation sequencing (NGS)Basic Protocol 5: NGS analysis of naïve and enriched libraries