bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistence of inert macromolecules (imposil) in the rat mesangium and glomerular functional disturbance
Authors: Goode N. P. , Davison A. M. , Gowland G. , Aparicio S. R. , Shires M. 
Published: June 2005

AbstractImposil iron—dextran is an inert tracer that has been used to study mesangial uptake and clearance of macromolecular material from the glomerular circulation. Such a tracer may be a useful marker of altered mesangial function in animals with some forms of glomerulonephritis. We have studied mesangial handling of intravenously injected Imposil (50 mg/100 g body weight) in normal rats by light, immunofluorescence and electron microscopy for up to 3 months. Mesangial cell uptake was maximal at 48–54 h. Extrusion and drainage of tracer to the vascular pole and distal tubule was evident at 3 days but iron was still present in mesangial cells at 3 months. Possible functional renal impairment resulting from persistent mesangially sequestered tracer was examined by measuring daily urine protein and iron excretion. A possible relationship between failure of mesangial cells to eliminate inert tracer and increasing glomerular permeability is demonstrated, suggesting that Imposil and similar inert macromolecules cannot be used for long‐term studies of mesangial function.
Spindle cell tumours of the skin of debatable origin. An immunocytochemical study
Authors: Eusebi V. , Ceccarelli C. , Piscioli F. , Cristofolini M. , Azzopardi J. G. 
Published: June 2005

AbstractTwelve cases of malignant spindle‐cell and sarcomatoid tumours of the skin of debatable nature were studied by immunocytochemical methods, using four antisera which might help contribute to resolution of the problems. The initial diagnosis made on structural grounds was confirmed by immunocytochemistry in six of eight cases in which a specific diagnosis had been made (one melanoma, three squamous carcinomas and two atypical fibroxanthomas). One case, initially regarded as AFX was reclassified as a squamous carcinoma, while a further case of possible AFX could not be confirmed by immunocytochemical study. Of the four cases in which structural examination was inconclusive, two were identified as squamous carcinomas and one as a melanoma by virtue of tumour markers. The fourth case was an intriguing actin‐rich tumour of uncertain nature. Immunocytochemistry, despite certain limitations, has a valuable role to play in the analysis of the problematic spindle‐cell malignant and pseudomalignant tumours of the skin.
The application of the metalophil method for the demonstration of histiocytes
Authors: van Unnik J. A. M. 
Published: June 2005

AbstractThe metalophil method has been performed on 250 sections of a wide variety of inflammatory and neoplastic lesions in which different types of histiocytes might be encountered. In lymph nodes, dendritic histiocytes related to the B‐lymphocyte system were consistently metalophil. They appeared as small cells with slender extensions in lymph nodes with follicular hyperplasia and/or sinus histiocytosis and in some cases of Hodgkin's lymphomas or as larger pleomorphic cells in primary or secondary malignancies of lymph nodes. Small, rounded cells were seen in some cases of marked paracortical reaction, in dermatopathic lymphadenitis and in some cases of mycosis fungoides. These cells most probably represented Langerhans cells and interdigitating reticulum cells, which are related to the T‐lymphocyte system. Interdigitating cells become metalophil when they are activated or proliferating. Epithelioid cells in different benign and malignant lesions were metalophil like the sinus histiocytes of the lymph nodes, the Kupffer cells of the liver and the alveolar histiocytes in the lung. Foreign‐body giant cells in lymph nodes after lymphography were also metalophil. The sinus lining cells lymph nodes were also well‐delineated. Histiocytes of malignant histiocytic proliferations were sometimes metalophil as were the so‐called histiocytes in malignant fibrous histiocytomas. Epithelial cells, particularly the basal cells of squamous epithelium often take up the silver. Carcinoma cells were sometimes metalophil and the method appeared not to be of value in the differentiation between metastatic carcinomas and lymphomas. The most promising application seems to be the study of the distribution of dendritic histiocytes in malignant proliferations of B‐lymphocytes.
Focal lymphocytic thyroiditis in southampton
Authors: Mitchell J. D. , Kirkham N. , Machin David 
Published: June 2005

AbstractThe presence of focal collections of lymphocytes within the thyroid gland has been associated with autoimmune thyroiditis. In a prospective study 197 thyroid glands were examined from post mortems performed in two Southampton hospitals over a 12 month period. The mean thyroid mass was 19·5 g in women and 22·2 g in men. Varying degrees of focal lymphocytic thyroiditis were present in 54 per cent of women and 24 per cent of men. Morphometry showed that on average less than 4 per cent of the gland was infiltrated by lymphocytes. There was no significant relationship with age. A possible association between focal lymphocytic thryoiditis and circulating thyroid autoantibodies has been shown.
Small cell tumours in childhood: A review
Authors: Variend S. 
Published: June 2005

AbstractThe past decade has seen significant advances in the treatment of childhood malignancies accompanied by appreciable improvement in survival rates. Treatment programmes have been largely formulated to meet the specific characteristics of individual tumours, as well as being based on the extent of disease presented at diagnosis. In selecting the most appropriate treatment protocol, accurate histological categorization of resected or biopsied tumour is thus of paramount importance. In the paediatric age range in which so many tumours lack differentiation as to present as, or mimic, other small cell tumours, routine methods are often insufficient to resolve problematic histology. A wide range of special techniques is now at hand to assist the pathologist with this problem and this review is an attempt partly to rationalize the application of available methodology. Of considerable importance also is a knowledge of the behavioural characteristics of this group of tumours, their prototypic histology, as well as the range of morphological variability.
Immunohistochemical analysis of human MHC class II antigens in B‐cell non‐Hodgkin's lymphomas
Authors: Krajewski A. S. , Guy K. , Dewar A. E. , Cossar D. 
Published: June 2005

AbstractTwenty cases of B‐cell non‐Hodgkin's lymphoma (NHL) were evaluated by immunohistochemical staining of frozen sections with a panel of anti MHC class II monoclonal antibodies (MCA). These studies showed marked differences in the expression of class II antigens both between different cases and within the population of cells of individual cases. In all of the cases studied the majority of the tumour cells reacted with MCAs directed against determinants common to the products of SB and DR loci. However, MCAs specific for DC determinants failed to react with 3/20 cases and in several other cases stained only a minority of cells. Absent or reduced expression of DC antigens was most marked in lymphocytic lymphoma; however, in centroblastic‐centrocytic and centroblastic lymphomas, DC antigens could be detected on the majority of cells.
Paraganglioma of the cauda equina: A histological, ultrastructural and immunocytochemical study of two cases with a review of the literature
Authors: Ironside J. W. , Royds Janice A. , Taylor C. B. , Timperley W. R. 
Published: June 2005

AbstractThe clinical and pathological features of two paragangliomas arising in the cauda equina are described and compared with 14 previous reports. The light microscopic features were similar to those of paragangliomas from other sites, with a ‘Zellballen’ pattern of cells containing argyrophil granules. Electron microscopy showed densely staining membrane‐bound granules, cilia and fibrous bodies in the cytoplasm. The last two features only occur in paragangliomas from this site. γ‐Enolase was demonstrated by the peroxidase‐antiperoxidase technique for the first time in these neoplasms. This technique was also used to demonstrate cytokeratins, which appear to be associated with the presence of fibrous bodies. The pathological findings suggest that paragangliomas in this site arise from pre‐existing paraganglia, possibly of the visceral autonomic group. The prognosis in cases treated by complete excision appears to be good.
The glomerular ultrastructural distribution of immunoglobulin g in hyperalbuminaemic (protein‐overload) proteinuria
Authors: Howie A. J. , Bliss D. J. , Brewer D. B. 
Published: June 2005

AbstractFemale Munich‐Wistar rats were given intraperitoneal injections either of bovine serum albumin to induce proteinuria or of water as a control. Their kidneys were fixed in situ. An ultrastructural technique was used to demonstrate IgG antiperoxidase antibodies either injected from a heterologous species or autologous, induced by immunization with horseradish peroxidase. Photometry of electron micrographic negatives was used to determine the distribution of antiperoxidase antibodies. In glomeruli of control animals IgG was present in the basement membrane. There were three sites at which the passage of IgG across the basement membrane was hindered: between blood plasma and the lamina rara interna, between the lamina densa and the lamina rara externa, and between the lamina rara externa and the urinary space. Glomeruli of proteinuric animals were variable in appearance, some showing little structural damage and others showing marked changes with loss of epithelial foot processes and accumulation of vacuoles and protein droplets in epithelial cells. Both types of glomeruli contained IgG in the urinary space. The distribution of IgG in the basement membrane of both types was similar. Compared with control animals there was less IgG in the basement membrane and IgG was distributed uniformly across the basement membrane. The proteinuria in hyperalbuminaemia (protein‐overload) is associated with a diffuse change in the barrier function of the glomerular basement membrane to IgG which is, at least in the initial stages, not related to structural changes in glomerular epithelial cells.
Induction of nephroblastoma‐like renal tumours in rats by a single dose of 1,2‐dimethylhydrazine
Authors: Sadrudin A. A. , Lewin M. R. , Cruse J. P. 
Published: June 2005

AbstractA single injection of 1,2‐dimethylhydrazine (200 mg/kg s.c.) resulted in the development of renal tumours in 70 per cent of treated rats within 1 year. The tumours were frequently bilateral (36 per cent) and grew to a large size (up to 100 g) before causing death. Lung metastases were found in one case. Histologically, the majority of the tumours (73 per cent) exhibited the classical triphasic morphological features of nephroblastoma (Wilms' tumour) with varying proportions of epithelial (glomeruloid and tubular structures), stromal (fibrous tissue, smooth muscle, cartilage) and blastematous elements. This pathological resemblance to human nephroblastoma suggests the possibility of using dimethylhydrazine‐induced renal tumours as a model of Wilms' tumour.
The effects of calcium regulating hormones on bone resorption by isolated human osteoclastoma cells
Authors: Chambers T. J. , Fuller K. , McSheehy P. M. J. , Pringle J. A. S. 
Published: June 2005

AbstractCells were disaggregated from osteoclastomas, and the response of the giant cells to calcium‐regulating hormones, prostaglandin (PG)E1 and dibutyryl cyclic AMP (dbcAMP) was observed by phase‐contrast time‐lapse video microscopy. The pattern and nature of their response was very similar to that previously found to be characteristic of osteoclasts: calcitonin (CT), PGE1, and dbcAMP induced cytoplasmic quiescence, while parathyroid hormone (PTH) showed no influence on cytoplasmic motility or behaviour. The cells were also cultured on slices of devitalized cortical bone for 5 or 18 h. After this time the giant cells were associated with the appearance in the scanning electron microscope of characteristic resorption pits, the volume of which was calculated by computer‐assisted morphometric and stereophotogrammetric techniques after removal of cells. Calcitonin caused a dramatic reduction in the volume of bone resorbed by these isolated cells compared with control cultures, while PTH was without significant effect. This result supports the view that PTH does not increase bone resorption in intact bone through a direct effect on osteoclasts. PGE1 which stimulates bone resorption when added to intact bone, paradoxically reduced resorption in our cultures. It thus appears possible that PGE1 acts as a direct inhibitor of osteoclastic bone resorption but has an additional effect on other cells in bone, which are induced by PGE1 to cause osteoclastic stimulation.
Preparation and characterization of tetracycline‐loaded interpenetrating polymer networks of carboxymethyl cellulose and poly(acrylic acid): water sorption and drug release study
Authors: Bajpai Anil Kumar , Mishra Abhilasha 
Published: June 2005

AbstractTetracycline (TC)‐loaded ionic interpenetrating polymer networks (IPNs) of carboxymethyl cellulose (CMC) and crosslinked poly(acrylic acid) (PAA) were prepared and characterized by infrared spectral analysis, differential scanning calorimetry and scanning electron microscopy techniques. The prepared IPNs were evaluated for in vitro blood compatibility by clot formation and hemolysis methods and their water imbibitions capacity was determined. Fractional release dynamics of tetracycline was also investigated from loaded IPNs of CMC and PAA. The entrapped drug was examined for antibacterial activity and structural integrity and effects of various parameters such as percentage loading of the drug, chemical composition of the carrier IPN, pH and temperature of the release medium were investigated on the release profiles of TC. The drug was also released in different simulated biological fluids. Copyright © 2005 Society of Chemical Industry
Structural and end‐group effects on bulk and surface properties of hyperbranched poly(urea urethane)s
Authors: Elrehim Mona Abd , Voit Brigitte , Bruchmann Bernd , Eichhorn Klaus‐Jochen , Grundke Karina , Bellmann C. 
Published: June 2005

AbstractThe hydroxy end groups of aromatic and aliphatic hyperbranched poly‐(urea urethane)s prepared with an AA* + B*B2 one‐pot method were modified with phenylisocyanate, butylisocyanate, and stearylisocyanate. The success of the modification reaction was verified with 1H NMR and IR spectroscopy. Linear model poly‐(urea urethane)s were prepared, too, for comparison. The bulk properties of OH functionalized hyperbranched poly(urea urethane)s, compared with those of linear analogues and modified hyperbranched poly(urea urethane)s, were studied with differential scanning calorimetry, thermogravimetric analysis, and temperature‐dependent Fourier transform infrared measurements. Transparent and smooth thin films could be prepared from all polymer samples and were examined with a light microscope, a microglider, and an atomic force microscope. The properties of the polymer surface were examined by measurements of the contact angle and zeta potential. For all samples, the properties were mainly governed by the strong interactions of the urea and urethane units within the backbone, whereas the influence of the nature of the end groups and of the branched structure was reduced in comparison with other hyperbranched polymer systems. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 3376–3393, 2005
Molecular modeling of cardiac glycoside binding by the human sequence monoclonal antibody 1B3
Authors: Paula Stefan , Monson Nancy , Ball William James 
Published: June 2005

AbstractThe amino acid sequences of the heavy‐ and light‐chain variable regions of the high‐affinity human sequence antidigoxin monoclonal antibody 1B3 (mAb 1B3) were determined, and a structural model for the mAb's variable region was developed by homology modeling techniques. The structural model provided the basis for computationally docking digoxin and eight related cardiac glycosides into the putative binding site of mAb 1B3. Analysis of the consensus binding mode obtained for digoxin showed that the cardenolide moiety of digoxin is deeply embedded in a predominantly hydrophobic, narrow cavity, whereas the terminal, γ‐carbohydrate group is solvent‐exposed. The docking results indicated that the primary driving forces for digoxin binding by mAb 1B3 are hydrophobic interactions with the digoxin steroid ring system and hydrogen bonds with the digitoxose groups. The binding model accounts for the experimentally observed variations in mAb 1B3 binding affinity for various structural analogs of digoxin used previously to develop a 3D structure–activity relationship model of drug binding (Farr CD, Tabet MR, Ball WJ Jr, Fishwild DM, Wang X, Nair AC, Welsh WJ. Three‐dimensional quantitative structure–activity relationship analysis of ligand binding to human sequence antidigoxin monoclonal antibodies using comparative molecular field analysis. J Med Chem 2002;45:3257–3270). In particular, the hydrogen bond pattern is consistent with the unique sensitivity of mAb 1B3's binding affinity to the number of sugar residues present in a cardiac glycoside. The hydrophobic environment about the steroid moiety of digoxin is compatible with the mAb's reduced affinity for ligands that possess hydrophilic hydroxyl and acetyl group modifications in this region. The model also indicated that most of the amino acid residues in contact with the ligand reside in or about the three complementarity determining regions (CDRs) of the heavy chain and the third CDR of the light chain. A comparison of the 1B3 binding model with the crystal structures of two murine antidigoxin mAbs revealed similar binding patterns used by the three mAbs, such as a high frequency of occurrence of aromatic, hydrophobic residues in the CDRs and a dominant role of the heavy chain CDR3 in antigen binding. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Increasing the molecular contacts between maurotoxin and Kv1.2 channel augments ligand affinity
Authors: M'Barek Sarrah , Chagot Benjamin , Andreotti Nicolas , Visan Violeta , Mansuelle Pascal , Grissmer Stephan , Marrakchi Mohamed , El Ayeb Mohamed , Sampieri François , Darbon Hervé , Fajloun Ziad , De Waard Michel , Sabatier Jean‐Marc 
Published: June 2005

AbstractScorpion toxins interact with their target ion channels through multiple molecular contacts. Because a “gain of function” approach has never been described to evaluate the importance of the molecular contacts in defining toxin affinity, we experimentally examined whether increasing the molecular contacts between a toxin and an ion channel directly impacts toxin affinity. For this purpose, we focused on two scorpion peptides, the well‐characterized maurotoxin with its variant Pi1‐like disulfide bridging (MTXPi1), used as a molecular template, and butantoxin (BuTX), used as an N‐terminal domain provider. BuTX is found to be 60‐fold less potent than MTXPi1 in blocking Kv1.2 (IC50 values of 165 nM for BuTX versus 2.8 nM for MTXPi1). Removal of its N‐terminal domain (nine residues) further decreases BuTX affinity for Kv1.2 by 5.6‐fold, which is in agreement with docking simulation data showing the importance of this domain in BuTX‐Kv1.2 interaction. Transfer of the BuTX N‐terminal domain to MTXPi1 results in a chimera with five disulfide bridges (BuTX‐MTXPi1) that exhibits 22‐fold greater affinity for Kv1.2 than MTXPi1 itself, in spite of the lower affinity of BuTX as compared to MTXPi1. Docking experiments performed with the 3‐D structure of BuTX‐MTXPi1 in solution, as solved by 1H‐NMR, reveal that the N‐terminal domain of BuTX participates in the increased affinity for Kv1.2 through additional molecular contacts. Altogether, the data indicate that acting on molecular contacts between a toxin and a channel is an efficient strategy to modulate toxin affinity. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Binding MOAD (Mother Of All Databases)
Authors: Hu Liegi , Benson Mark L. , Smith Richard D. , Lerner Michael G. , Carlson Heather A. 
Published: June 2005

AbstractBinding MOAD (Mother of All Databases) is the largest collection of high‐quality, protein–ligand complexes available from the Protein Data Bank. At this time, Binding MOAD contains 5331 protein–ligand complexes comprised of 1780 unique protein families and 2630 unique ligands. We have searched the crystallography papers for all 5000+ structures and compiled binding data for 1375 (26%) of the protein–ligand complexes. The binding‐affinity data ranges 13 orders of magnitude. This is the largest collection of binding data reported to date in the literature. We have also addressed the issue of redundancy in the data. To create a nonredundant dataset, one protein from each of the 1780 protein families was chosen as a representative. Representatives were chosen by tightest binding, best resolution, etc. For the 1780 “best” complexes that comprise the nonredundant version of Binding MOAD, 475 (27%) have binding data. This significant collection of protein–ligand complexes will be very useful in elucidating the biophysical patterns of molecular recognition and enzymatic regulation. The complexes with binding‐affinity data will help in the development of improved scoring functions and structure‐based drug discovery techniques. The dataset can be accessed at http://www.BindingMOAD.org. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Phenotype analysis using network motifs derived from changes in regulatory network dynamics
Authors: Cavelier German , Anastassiou Dimitris 
Published: June 2005

AbstractThe intrinsic dynamic response of a transcriptional regulatory network depends directly on molecular interactions in the cellular transcription, translation, and degradation machineries. These interactions can be incorporated into dynamic mathematical models of the biochemical system using the biophysical relationship with the model parameters. Modifications of such interactions bring changes to the biological behavior of the cells, and therefore, many normal and pathological cellular states depend on them. It is important for analysis, prediction, diagnosis, and treatment of cellular function to have an experimentally derived model with parameters that adequately represent the molecular interactions of interest. Finding the model and parameters of a transcriptional regulatory network is a difficult task that has been approached at different levels and with different techniques. We develop here a new analysis method (based on previous work on network inference, modeling, and parameter identification) that finds the most changed parameters from yeast oligonucleotide microarray expression patterns in cases where a phenotype difference exists between two samples. We then relate and examine the changed parameters with their associated genes, corresponding genetic functional categories, and particular subnetworks and connectivities. The biophysical bases for these changes are also identified by studying the relationship of the changed parameters with the transcription, translation, and degradation mechanisms. The method is improved to cases where there are two or more transcription factors influencing transcription, and a statistical analysis is performed to give a measurement of the uniqueness and robustness of the parameter fit. Proteins 2005. © 2005 Wiley‐Liss, Inc.