bluuebunny/crossref_metadata_2025_split huggingface dataset:
Defining Clinical Endpoints in Limb Girdle Muscular Dystrophy: A GRASP-LGMD study
Authors: Doody Amy , Alfano Lindsay , Diaz-Manera Jordi , Lowes Linda , Mozaffar Tahseen , Mathews Kathy , Weihl Conrad C. , Wicklund Matthew , Statland Jeffery , Johnson Nicholas E. , Consortium GRASP-LGMD 
Published: October 2023

Abstract
Background
The Limb Girdle Muscular Dystrophies (LGMDs) are characterized by progressive weakness of the shoulder and hip girdle muscles as a result of over 30 different genetic mutations. This study is designed to develop clinical outcome assessments across the group of disorders.
Methods/design:
The primary goal of this study is to evaluate the utility of a set of outcome measures on a wide range of LGMD phenotypes and ability levels to determine if it would be possible to use similar outcomes between individuals with different phenotypes. We will perform a multi-center, 12-month study of 188 LGMD patients within the established Genetic Resolution and Assessments Solving Phenotypes in LGMD (GRASP-LGMD) Research Consortium, which is comprised of 11 sites in the United States and 2 sites in Europe. Enrolled patients will be clinically affected and have mutations in CAPN3 (LGMDR1), ANO5 (LGMDR12), DYSF (LGMDR2), DNAJB6 (LGMDD1), SGCA (LGMDR3), SGCB (LGMDR4), SGCD (LGMDR6), or SGCG (LGMDR5, or FKRP-related (LGMDR9).
Discussion
To the best of our knowledge, this will be the largest consortium organized to prospectively validate clinical outcome assessments (COAs) in LGMD at its completion. These assessments will help clinical trial readiness by identifying reliable, valid, and responsive outcome measures as well as providing data driven clinical trial decision making for future clinical trials on therapeutic agents for LGMD. The results of this study will permit more efficient clinical trial design. All relevant data will be made available for investigators or companies involved in LGMD therapeutic development upon conclusion of this study as applicable.
Trial registration:
clinicaltrials.gov NCT03981289; Date of registration: 6/10/2019
Comparison of indocyanine green and blue-stained glue for preoperative localization for pulmonary nodules
Authors: Lin Jia , Zhang Jia , Wei Ning , Wu An-Le , Wang Long-Fei , Teng Fei , Xian Yu-Tao , Han Rui 
Published: October 2023

Abstract
Background
In patients with pulmonary nodules undergoing computed tomography (CT)-guided localization procedures, a range of liquid-based materials have been employed to date in an effort to guide video-assisted thoracoscopic surgery (VATS) procedures to resect target nodules. However, the relative performance of these different liquid-based localization strategies has yet to be systematically evaluated. Accordingly, this study was developed with the aim of examining the relative safety and efficacy of CT-guided indocyanine green (IG) and blue-stained glue (BSG) PN localization.
Methods
Consecutive patients with PNs undergoing CT-guided localization prior to VATS from November 2021 - April 2022 were enrolled in this study. Safety and efficacy outcomes were compared between patients in which different localization materials were used.
Results
In total, localization procedures were performed with IG for 121 patients (140 PNs), while BSG was used for localization procedures for 113 patients (153 PNs). Both of these materials achieved 100% technical success rates for localization, with no significant differences between groups with respect to the duration of localization (P = 0.074) or visual analog scale scores (P = 0.787). Pneumothorax affected 8 (6.6%) and 8 (7.1%) patients in the respective IG and BSG groups (P = 0.887), while 12 (9.9%) and 10 (8.8%) patients of these patients experienced pulmonary hemorrhage. IG was less expensive than BST ($17.2 vs. $165). VATS sublobar resection procedure technical success rates were also 100% in both groups, with no instances of conversion to thoracotomy.
Conclusions
IG and BSG both offer similarly high levels of clinical safety and efficacy when applied for preoperative CT-guided PN localization, with IG being less expensive than BSG.
TC/(HDL-C+TBIL) and LDL-C/(HDL-C+TBIL) ratios predict early neurological deterioration in minor ischemic stroke：A retrospective study
Authors: Lu Dandan , Wang Meng , Li Mengmeng , Zu Sai , Wang Pei 
Published: October 2023

Abstract
Objective It is generally known that bilirubin and cholesterol can significantly affect the clinical outcome of minor ischemic stroke(MIS) .In other words,early neurological deterioration (END) is still difficult to be predicted which is usually occurs in MIS patients .Our aim was to study the correlation between END,total bilirubin and cholesterol( total cholesterol,TC, low density lipoprotein cholesterol,LDL-C,high density lipoprotein cholesterol,HDL-C) by TC/(HDL-C+TBIL) and LDL-C/(HDL-C+TBIL) ratios.
Methods This was a retrospective study.MIS Patients within 48 hours of onset were retrospective recruited from Baoding NO.1 Central Hospital. END was defined as an NIHSS score increased≥ 2 points or individual score of consciousness level and muscle strength increased ≥1 point or new neurological damage manifestations during the 72 hours.During the period,total cholesterol, low density lipoprotein cholesterol,high density lipoprotein cholesterol and total bilirubin , TC/(HDL-C+TBIL) and (LDL-C/(HDL-C+TBIL) ratios were calculated.
Results 149 patients were included, 39 cases(26.17%) had END(END group) and 110 cases(73.83%) did not had END(non-END group). In patients with non-END, smoking , TBIL were significantly higher than END(P＜0.05)，but systolic pressure, neutrophil ratio, fasting glucose,LDL-C/(HDL-C+TBIL), TC/(HDL-C+TBIL) were lower(P＜0.05). In tne logistic regression analysis LDL-C/( HDL-C+TBIL) (OR=1.061, 95%CI:1.013~1.111, P=0.012), TC/(HDL-C+TBIL) (OR=1.048, 95%CI:1.008~1.091, P=0.019) were independent risk factors for END. The receiver operated curve (ROC) showed that LDL-C/(HDL-C+TBIL) predicted END with sensitivity 53.8%, specificity 87.3% , TC/(HDL-C+TBIL) predicted END with sensitivity 64.1%, specificity 79.1% .
Conclusion END occurs 26.17% in minor ischemic stroke. TC/(HDL-C+TBIL) and (LDL-C/(HDL-C+TBIL) ratios were statistically significant predictor for END in MIS.
Configurational Effects of Intergenerational Support on older adults’ depression: An empirical study from CHARLS data
Authors: bai qin , Zhu Jinghui , fu duanduan , wang fang , gao baizhi , Liu Guilin 
Published: October 2023

Abstract

Background: Intergenerational support is a crucial factor influencing the psychological well-being of older adults. The interactions between various dimensions of intergenerational support and the diverse effects of different configuration of these factors on older adults’ depression still require further investigation.
Method: Utilizing the 2018 data from the China Health and Retirement Longitudinal Study (CHARLS), this study employs the Qualitative Comparative Analysis (QCA) method to analyze the configurational effects of intergenerational support on older adults’ depression.
Result: Single-dimensional intergenerational support cannot be considered a necessary condition for influencing older adults’ depression. The configurational effects of intergenerational support across different dimensions serve as sufficient conditions for older adults’ depression. The consistency parameter for all solution configurations in this study is 0.83, with a coverage of 0.61. The research identifies four types of configurational patterns associated with older adults’ depression: "Unidirectional Care Deficiency Type" (consistency mean of 0.84, coverage of 0.49), "Bidirectional Care Deficiency Type" (consistency mean of 0.86, coverage of 0.33), "Bidirectional Economic Deficiency Type" (consistency mean of 0.85, coverage of 0.48), and "Mixed Type" (consistency mean of 0.83, coverage of 0.23).
Conclusion: The configurational effects of intergenerational support across different dimensions contribute to an increase in the levels of older adults’ depression. It is advocated to adopt a holistic perspective when examining the intricate connection between intergenerational support and older adults’ depression. Furthermore, it is important to pay attention to maintaining a dynamic equilibrium between receiving and providing intergenerational support.
The impact of parental consanguineous marriage on the incidence rate, incidence age, and severity of coronary artery disease
Authors: Khederlou Hamid , Sadeghian Saeed , Jalali Arash , Mohammadi Amirali , Shokoeizadeh Fatemeh , Hamidabadi Nasrin , Yadangi Somayeh , Tajdini Masih 
Published: October 2023

Abstract

Background Consanguineous marriage (CM) 's impact on cardiovascular diseases, including structural congenital heart diseases, has been confirmed. Although CM can affect genetics with oligogenicity and homogeneity, the relationship between CM and coronary artery disease (CAD) has not been conclusively confirmed. Therefore, this study was conducted to determine the relationship between CAD and CM. Methods We conducted a cross-sectional-prospective study from June 2021 to August 2022. Patients undergoing coronary angiography at Tehran Heart Center were included. A questionnaire was designed, including demographic and anthropometric information, past medical history, cardiovascular risk factors, results of previous angiograms, and parental kinship. Finally, 11,986 patients were analyzed regarding the relationship between CAD and CM. Results 69.9% of the patients included in this study were male. The mean age of the patients was 58.9 ± 10.7 years. Out of 11986 patients, 9083 (75.8%) patients had CAD. One thousand five hundred fifteen (12.7%) patients were with parental CM. This study showed that the incidence of CAD in patients with parental CM is 3.2% higher than in patients with parental non-CM (P-value < 0.001). Also, CM reduces the mean age of CAD occurrence independently of other cardiovascular risk factors by 2.4 years [OR -2.4, 95% CI -3.5_-1.8, P-value < 0.001]. Conclusions The incidence of CAD in patients with parental CM is higher than in patients with parental non-CM. The parental CM reduces the age of onset of CAD in their children. Therefore, parental CM should be considered one of the risk factors for CAD.
Salvianolic Acid B (Sal B) ameliorates myocardial fibrosis in diabetic cardiomyopathy through deubiquitinating Smad7
Authors: Luo Hong , Fu Lingyun , Wang Xueting , Xu Yini , Tao Ling , Shen Xiangchun 
Published: October 2023

Abstract
Background Salvianolic acid B (Sal B), a water-soluble phenolic compound derived from Salvia Miltiorrhiza Bunge, is commonly used in Chinese Traditional Medicine for the treatment of cardiovascular diseases. Sal B has shown protecting effects against myocardial fibrosis induced by diabetic cardiomyopathy(DCM) in our previous experiment. This study aimed to investigate the ameliorative effects and potential mechanisms of Sal B in mitigating myocardial fibrosis induced by DCM.
Methods In this study, a variety of methods were utilized to investigate the effects of Sal B on improving myocardial fibrosis induced by DCM in vivo and in vitro. These methods included weight measurement, blood glucose analysis, echocardiography, HE staining, Masson's trichrome staining, Sirius red staining, cell proliferation assessment, determination of hydroxyproline levels, immunohistochemical staining, evaluation of fibrosis-associated protein expression (Collagen I, Collagen III, TGF-β1, p-Smad3, Smad3, Smad7, and α-smooth muscle actin), analysis of Smad7 gene expression, and analysis of Smad7 ubiquitin modification.
Results The animal test results indicated Sal B could significantly improve cardiac function, inhibit collagen deposition and phenotypic transformation, ameliorate myocardial fibrosis in DCM by up-regulating Smad7, thereby inhibiting TGF-β1 signaling pathway. Additionally, cell experiments demonstrated Sal B could significantly inhibit the proliferation, migration, phenotypic transformation and collagen secretion of cardiac fibroblasts (CFs) induced by high glucose(HG). Sal B could significantly decrease the ubiquitization modifications of Smad7, stabilize the protein expression of Smad7, therefore increase the protein expression of Smad7 in the CFs , inhibit TGF-β1 signaling pathway, that maybe a potential mechanism of Sal B in mitigating myocardial fibrosis induced by DCM.
Conclusion In summary, this study revealed that Salvianolic acid B can improve myocardial fibrosis in DCM by deubiquitinating Smad7, stabilizing the protein expression of Smad7, blocking the TGF-β1 signaling pathway.
Association between SMOFlipid and impaired brain development on term-equivalent age brain magnetic resonance imaging in very preterm infants
Authors: Al-Mouqdad Mountasser M. , Alshaikh Belal , Sumaily Haider H. , Alodhaidan Nabeel A. , AlMahmoud Latifah , Almotiri Ameen A. , Alkhourmi Mousa A. , Abounassif Mazen M. , Beh Ahmed F. , Alawad Mashael A , Alqarni Aziza A , Al-Anazi Maha R. , Asfour Suzan S. 
Published: October 2023

Abstract

Soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOFlipid) is used without evidence of benefits. We investigated the relationship between lipid emulsions and brain injury in term-equivalent age magnetic resonance imaging (MRI) in 148 very preterm infants with a birth weight of < 1500 g at ≤ 32 gestational weeks in a neonatal intensive care unit. Infants who received soybean-based lipid emulsions between January 2015 and December 2018 were compared with those who received SMOFlipids between January 2019 and December 2022. A negative binomial generalized linear model was applied for univariate analysis. Modified log-Poisson regression with generalized linear models and a robust variance estimator (Huber–White) were applied to adjust for potential confounders. The Kidokoro score was used to determine if lipid emulsion type would affect brain morphology and growth at term-equivalent age. Eighy-six (58.9%) received SMOFlipid. SMOFlipid was associated with lower focal signal abnormality, myelination delay, increased extracerebral space, and cerebellar volume reduction (P = 0.02, P = 0.007, P = 0.01, P = 0.02, respectively). SMOFlipidis are associated with brain insult, especially in white matter, cortical gray matter, and the cerebellum. Well-designed studies are needed to investigate the effect of lipid emulsions on the central nervous system.
Sleep duration and cognitive function among rural older adults in China: a population- based study
Authors: Fang Yongxu , Yan Zhongrui , Wang Xinglu , She Rui , Wang Peng , Liang Yajun 
Published: October 2023

Abstract

Background The appropriate sleep duration is essential for maintaining normal cognitive function, but evidence is scarce in rural elderly population. This study investigates the associations between self-reported sleep duration and cognitive function among older adults in rural China. Methods This population-based cross-sectional study used data from the Confucius Hometown Aging Project in Shandong, China. Data on demographics, lifestyles, and chronic health conditions were collected through interviews, clinical examinations, and laboratory tests. Sleep duration was classified into four groups (≤ 5, 6, 7, and ≥ 8 h/day). The Mini-Mental State Examination (MMSE) was used to assess the global and domain-specific cognitive function. Linear and logistic regression were performed to determine the associations between sleep duration and cognitive function. Results There was a U-shaped relationship between sleep duration and cognitive impairment. Compared with 6 hours’ sleep per day, sleeping ≤ 5 hours and ≥ 8 hours per day was associated with cognitive impairment, with odds ratio (95% confident interval) being 1.66 (1.02, 2.70) and 1.77 (1.07, 2.92) respectively. Compared with those sleep 6 hours per day, those sleep 5 hours per day or less had lower score of attention and calculation (β = -0.21, 95% confidence interval= -0.38, -0.05), those sleep 8 hours or more had lower score of orientation, attention and calculation, delayed recall, and linguistic competence (β range from − 0.08 to -0.22). Conclusions There is a U-shape relationship between sleep duration and cognitive impairment in rural Chinese older adults.
PHF12 regulates HDAC1 to promote tumorigenesis via EGFR/AKT signaling pathway in non-small cell lung cancer
Authors: Kong Yiru , jiang rongrong , Zhou Hui , Ge Mengxi , Lin Hao , Wang Yu , Yao Rongrong , Wang Qing , Liang Xiaohua , Li Jing , Zhou Xinli 
Published: October 2023

Abstract

Background:
Lung cancer stands as the second most prevalent malignant neoplasm worldwide. Addressing the underlying mechanisms propelling the progression of non-small cell lung cancer is of paramount importance. In this study, we have elucidated the pivotal role of PHF12 in this context.
Materials and Methods:
We harnessed clinical lung cancer tissue samples and non-small cell lung cancer cell lines to discern the expression pattern of PHF12. In vitro assays probing cell proliferation were conducted to substantiate the functional impact of PHF12. Furthermore, an in vivo Xenograft model was employed to dissect the role of PHF12. Employing ChIP assays and qRT-PCR, we delved into the intricate binding dynamics between PHF12 and HDAC1. Mechanistic insights into the PHF12-HDAC1 axis in lung cancer progression were pursued via RNA-seq and GSEA analyses.
Results
Notably, PHF12 exhibited a substantial upregulation within tumor tissue, concomitant with its correlation to HDAC1. The trilogy of cell proliferation assays, transwell assays, and the Xenograft model collectively underscored the promoting influence of PHF12 on lung cancer proliferation, both in vitro and in vivo. The ChIP assay unveiled the transcriptional regulatory role of PHF12 in governing HDAC1 expression. This correlation extended to both mRNA and protein levels. Intriguingly, the rescue of function within NSCLC cell lines post PHF12 knockdown was achievable through HDAC1 overexpression. Additionally, our findings unveiled the capacity of the PHF12-HDAC1 axis to activate the EGFR/AKT signaling pathway, thereby further corroborating its significance in lung cancer progression.
Conclusion
Our study identified PHF12 as an oncogenic role in lung cancer proliferation for the first time. PHF12 transcriptionally regulate HDAC1 and activate EGFR/AKT signaling pathway in NSCLC progression. PHF12 may serve as an important target in lung cancer therapy.
Identification of genomic regions for deep-water resistance in rice for efficient weed control with reduced herbicide use
Authors: Iwasa Marina , Chigira Koki , Nomura Tomohiro , Adachi Shunsuke , Asami Hidenori , Nakamura Tetsuya , Motobayashi Takashi , Ookawa Taiichiro 
Published: October 2023

Abstract
Deep-water (DW) management in rice fields is a promising technique for efficient control of paddy weeds with reduced herbicide use. Maintaining a water depth of 10–20 cm for several weeks can largely suppress the weed growth, though it also inhibits rice growth because the DW management is usually initiated immediately after transplanting. Improving the DW resistance of rice during the initial growth stage is essential to avoid suppressing growth. In this study, we demonstrate a large genetic variation in the above-ground biomass (AGB) after the end of DW management among 165 temperate japonica varieties developed in Japan. Because the AGB closely correlated with plant length (PL) and tiller number (TN) at the early growth stage, we analyzed genomic regions associated with PL and TN by conducting a genome-wide association study. For PL, a major peak was detected on chromosome 3 (qPL3), which includes a gene encoding gibberellin biosynthesis, OsGA20ox1. The rice varieties with increased PL had a higher expression level of OsGA20ox1 as reported previously. For TN, a major peak was detected on chromosome 4 (qTN4), which includes NAL1 gene associated with leaf morphological development and panicle number. Although there was less difference in the expression level of NAL1 between genotypes, our findings suggest that an amino acid substitution in the exon region is responsible for the phenotypic changes. We also found that the rice varieties having alternative alleles of qPL3 and qTN4 showed significantly higher AGB than the varieties with the reference alleles. Our results suggest that OsGA20ox1 and NAL1 are promising genes for improving DW resistance in rice.
Evolution of Stakeholder Relationships in Green Ship Construction
Authors: Geng Can , Kuang Haibo 
Published: October 2023

Abstract

With the increasing severity of environmental issues, the relevant maritime organizations have gradually improved environmental requirements for the shipping industry to reduce pollution emissions. The environmental benefits brought by green vessels have been proven globally. However, due to the public nature of green vessel construction, shipowners do not proactively engage in green vessel development. To encourage voluntary participation and prevent shipowners from evading green supervision by seeking rent from inspection agencies, it is necessary to explore the cooperative relationship among shipowners, inspection agencies, and governments, as well as the roles of each participant in the construction process. This study uses dynamic simulation methods to analyze the game relationship among relevant stakeholders. The analysis follows the classic assumptions of game theory, assuming that players are rational and have common knowledge. The results show that reasonable reward and punishment mechanisms as well as strict supervision can promote the stable development of the tripartite game system. Additionally, this research proposes several incentive measures for the government to promote green vessel construction, including adjusting policy rewards, punishments and strengthening supervision mechanisms for different participants.
Persistently smaller tassels threaten maize yield under a warming climate
Authors: Huang Shoubing , Zhang Yingjun , Dong Xin , Wang Hongyu , Lin Yihsuan , Jin Lian , Lv Xuanlong , Yao Qian , Li Baole , Gao Jia , Wang Pu , Wang Baobao 
Published: October 2023

Abstract

Heat waves become more serious with the warming climate, increasing the demand for developing high temperature (HT) tolerant maize germplasm. Here we compared the responses of 323 elite inbred lines released in multiple eras from both China and the United States to HT during flowering under field conditions. The newly released lines exhibit higher grain yield than the early released lines as a result of improved ear characteristics and flowering synchrony. However, the newly released lines are more susceptible to HT stress partly due to the reduced tassel size and spikelet opening angle. We identify a key threshold for spikelet/tassel (~700), over which maize can produce a stably high seed set under HT stress. According to the daily temperature during flowering, it is estimated that small-tassel (<700 spikelet/tassel) genotypes are unsuitable in 23.7% of global maize-growing regions. This work provides important information for breeding and selecting HT-tolerant maize varieties.