bluuebunny/crossref_metadata_2025_split huggingface dataset:
LIBS as a novel tool for the determination of the imidization degree of polyimides
Authors: Achleitner Birgit , Girault Laurie , Larisegger Silvia , Nelhiebel Michael , Knaack Patrick , Limbeck Andreas 
Published: February 2024

AbstractDue to their outstanding chemical and physical properties, polyimides are widely used in industrial applications. The degree of imidization of polyimides significantly influences their properties, making it an important factor in tailoring the material for specific applications. Imidization refers to the process of converting a precursor poly(amic acid) by removing water, and it is essential to analyze this process in detail to tune the final structure and properties of the material. Conventional techniques for this task include Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), or differential scanning calorimetry (DSC), but they lack the possibility of spatially and/or depth-resolved analysis or do not enable in-line monitoring capabilities. To overcome these limitations, we propose laser-induced breakdown spectroscopy (LIBS) as a powerful tool for the monitoring of the imidization reaction. To establish a measurement method, a total of 130 in-house prepared, self-synthesized samples were thermally cured to exhibit varying imidization degrees. IR spectroscopy served as a reference technique during method development, and a novel formula for calculating the degree of imidization, based on the C2 and H signal trends, was introduced. The calculated imidization degrees of model thin films based on LIBS were in good accordance with the IR reference method although minor differences between the two methods were expected due to varying information depth and the size of the sampled area. Additionally, the robustness of the procedure was demonstrated by depth profiling of a stacked model polymer, spiking with commercially available additives and, ultimately, by analyzing industry-relevant polymer samples.
Graphical Abstract
Oxidative stress resistance prompts pyrroloquinoline quinone biosynthesis in Hyphomicrobium denitrificans H4-45
Authors: Liang Jiale , Tang Mingjie , Chen Lang , Wang Wenjie , Liang Xinle 
Published: February 2024

Abstract
Pyrroloquinoline quinone (PQQ) is a natural antioxidant with diverse applications in food and pharmaceutical industries. A lot of effort has been devoted toward the discovery of PQQ high-producing microbial species and characterization of biosynthesis, but it is still challenging to achieve a high PQQ yield. In this study, a combined strategy of random mutagenesis and adaptive laboratory evolution (ALE) with fermentation optimization was applied to improve PQQ production in Hyphomicrobium denitrificans H4-45. A mutant strain AE-9 was obtained after nearly 400 generations of UV-LiCl mutagenesis, followed by an ALE process, which was conducted with a consecutive increase of oxidative stress generated by kanamycin, sodium sulfide, and potassium tellurite. In the flask culture condition, the PQQ production in mutant strain AE-9 had an 80.4% increase, and the cell density increased by 14.9% when compared with that of the initial strain H4-45. Moreover, batch and fed-batch fermentation processes were optimized to further improve PQQ production by pH control strategy, methanol and H2O2 feed flow, and segmented fermentation process. Finally, the highest PQQ production and productivity of the mutant strain AE-9 reached 307 mg/L and 4.26 mg/L/h in a 3.7-L bioreactor, respectively. Whole genome sequencing analysis showed that genetic mutations in the ftfL gene and thiC gene might contribute to improving PQQ production by enhancing methanol consumption and cell growth in the AE-9 strain. Our study provided a systematic strategy to obtain a PQQ high-producing mutant strain and achieve high production of PQQ in fermentation. These practical methods could be applicable to improve the production of other antioxidant compounds with uncleared regulation mechanisms.

Key points
• Improvement of PQQ production by UV-LiCl mutagenesis combined with adaptive laboratory evolution (ALE) and fermentation optimization.
• A consecutive increase of oxidative stress could be used as the antagonistic factor for ALE to enhance PQQ production.
• Mutations in the ftfL gene and thiC gene indicated that PQQ production might be increased by enhancing methanol consumption and cell growth.
Candidate Gene Transcriptional Signature Unravels the Reprogramming Occurring in the Peel of Apple Fruit of ‘Granny Smith’ During Postharvest Storage
Authors: Vittani Lorenzo , Populin Francesca , Stuerz Stefan , Fava Federico , Robatscher Peter , Zanella Angelo , Costa Fabrizio , Busatto Nicola 
Published: February 2024

AbstractAfter harvest fruit are stored to preserve the quality features established during the on-tree development and maturation, ensuring thus a continuous availability of fresh fruit on the market. For certain fruit species like apple, storage can last for almost a year, especially when coupled with several strategies, such as the reduction of the oxygen concentration or the application of ethylene competitor molecules, like 1-methylcyclopropene (1-MCP). To guarantee the maintenance of the highest quality, the monitoring of the physiological processes ongoing during the postharvest ripening is compelling. For this purpose, 16 genes belonging to key fruit ripening pathways, such as the ethylene and the sugar/fermentation metabolism, have been chosen as potential markers for the molecular characterization of the major changes occurring in the fruit during storage. Among these genes, ACS, PPO, PG1, RAP2-like, and ADH exhibited the most significant differential expression across the various samples. Based on the transcriptional pattern, this set of genes constitutes a valuable molecular tool for a precise and reliable RNA-based monitoring of the postharvest ripening progression and fermentation process in apples. PPO, together with S6PDH, were furthermore employed to inspect the onset of the superficial scald in apple and resulted to correlate with the evaluation of the incidence of this disorder and the accumulation of the sugar alcohol sorbitol, known to play important protecting roles to chilling injuries. The assessment of the transcriptional signature of these elements can facilitate the development of gene expression markers suitable for a more informed investigation of the physiological progression of the postharvest ripening in apples, ultimately leading to the promotion of high-quality stored apples, extending storage time while minimizing postharvest disorders and fruit loss.
Vestibular function and hearing preservation in children following a minimally invasive cochlear implantation
Authors: Wang Ruijie , Xu Kaifan , Luo Jianfen , Chao Xiuhua , Hu Fangxia , Zhang Daogong , Chen Yueling , Li Yuanling , Fan Zhaomin , Wang Haibo , Xu Lei 
Published: February 2024

Abstract
Purpose
This retrospective cohort study aimed to investigate the effect of minimally invasive cochlear implantation (CI) on the vestibular function (VF) and residual hearing (RH) as well as their relationship in pediatric recipients before and after surgery.

Methods
Twenty-four pediatric patients with preoperative low frequency residual hearing (LFRH) (250 or 500 Hz ≤ 80 dB HL) who underwent minimally invasive CI were enrolled. Pure-tone thresholds, the cervical/ocular vestibular-evoked myogenic potential (cVEMP/oVEMP), and video head impulse test (vHIT) were all evaluated in the 24 pediatric patients with preoperative normal VF before and at 1 and 12 months after surgery. The relationship between changes in hearing and VF was analyzed preoperatively and at 1 and 12 months postoperatively.

Results
There were no significant differences on VF preservation and hearing preservation (HP) at both 1 and 12 months post-CI (p > 0.05). At 1 month post-CI, the correlations of the variations in vestibulo-ocular reflex (VOR) gains of horizontal semicircular canal (HSC) and posterior semicircular canal (PSC) and the shift in 250 Hz threshold were negatively correlated (r = − 0.41, p = 0.04 and r = − 0.43, p = 0.04, respectively). At 12 months post-CI, the shift in 250 Hz threshold negatively correlated to the variations in VOR gain of superior semicircular canal (SSC) (r = − 0.43, p = 0.04); the HP positively correlated to the variation in oVEMP-amplitude ratio (AR) (r = 0.41, p = 0.04).

Conclusion
Our study confirmed that there were partial correlations between VF preservation and HP both in the short- and long-terms after atraumatic CI surgery, especially with the 250 Hz threshold. Regarding the variation of PSC function, the correlation with hearing status was variable with time after atraumatic CI surgery. Minimally invasive techniques for HP are successful and effective for the preservation of VF in pediatric patients both in the short- and long-terms.
Calcium imaging of adult olfactory epithelium reveals amines as important odor class in fish
Authors: Dieris M. , Kowatschew D. , Hassenklöver T. , Manzini I. , Korsching S. I. 
Published: February 2024

AbstractThe odor space of aquatic organisms is by necessity quite different from that of air-breathing animals. The recognized odor classes in teleost fish include amino acids, bile acids, reproductive hormones, nucleotides, and a limited number of polyamines. Conversely, a significant portion of the fish olfactory receptor repertoire is composed of trace amine-associated receptors, generally assumed to be responsible for detecting amines. Zebrafish possess over one hundred of these receptors, but the responses of olfactory sensory neurons to amines have not been known so far. Here we examined odor responses of zebrafish olfactory epithelial explants at the cellular level, employing calcium imaging. We report that amines elicit strong responses in olfactory sensory neurons, with a time course characteristically different from that of ATP-responsive (basal) cells. A quantitative analysis of the laminar height distribution shows amine-responsive cells undistinguishable from ciliated neurons positive for olfactory marker protein. This distribution is significantly different from those measured for microvillous neurons positive for transient receptor potential channel 2 and basal cells positive for proliferating cell nuclear antigen. Our results suggest amines as an important odor class for teleost fish.
The Time Complexity of Consensus Under Oblivious Message Adversaries
Authors: Winkler Kyrill , Paz Ami , Galeana Hugo Rincon , Schmid Stefan , Schmid Ulrich 
Published: February 2024

AbstractWe study the problem of solving consensus in synchronous directed dynamic networks, in which communication is controlled by an oblivious message adversary that picks the communication graph to be used in a round from a fixed set of graphs $$\textbf{D}$$
D
arbitrarily. In this fundamental model, determining consensus solvability and designing efficient consensus algorithms is surprisingly difficult. Enabled by a decision procedure that is derived from a well-established previous consensus solvability characterization for a given set $$\textbf{D}$$
D
, we study, for the first time, the time complexity of solving consensus in this model: We provide both upper and lower bounds for this time complexity, and also relate it to the number of iterations required by the decision procedure. Among other results, we find that reaching consensus under an oblivious message adversary can take exponentially longer than both deciding consensus solvability and broadcasting the input value of some unknown process to all other processes.
Hemodiafiltration for children with stage 5 chronic kidney disease: technical aspects and outcomes
Authors: Ahlmann Charlotte , Stronach Lynsey , Waters Kathryn , Walker Kate , Oh Jun , Schmitt Claus Peter , Ranchin Bruno , Shroff Rukshana 
Published: February 2024

AbstractDespite significant medical and technical improvements in the field of dialysis, the morbidity and mortality among patients with chronic kidney disease (CKD) stage 5 on dialysis remains extremely high. Hemodiafiltration (HDF), a dialysis method that combines the two main principles of hemodialysis (HD) and hemofiltration—diffusion and convection—has had a positive impact on survival when delivered with a high convective dose. Improved outcomes with HDF have been attributed to the following factors: HDF removes middle molecular weight uremic toxins including inflammatory cytokines, increases hemodynamic stability, and reduces inflammation and oxidative stress compared to conventional HD. Two randomized trials in adults have shown improved survival with HDF compared to high-flux HD. A large prospective cohort study in children has shown that HDF attenuated the progression of cardiovascular disease, improved bone turnover and growth, reduced inflammation, and improved blood pressure control compared to conventional HD. Importantly, children on HDF reported fewer headaches, dizziness, and cramps; had increased physical activity; and improved school attendance compared to those on HD. In this educational review, we discuss the technical aspects of HDF and results from pediatric studies, comparing outcomes on HDF vs. conventional HD. Convective volume, the cornerstone of treatment with HDF and a key determinant of outcomes in adult randomized trials, is discussed in detail, including the practical aspects of achieving an optimal convective volume.
Risk factors for prolonged length of stay after first single-level lumbar microdiscectomy
Authors: Ritter Leonard , Liebert Adrian , Eibl Thomas , Schmid Barbara , Steiner Hans-Herbert , Kerry Ghassan 
Published: February 2024

Abstract

Objective
The objective is to identify risk factors that potentially prolong the hospital stay in patients after undergoing first single-level open lumbar microdiscectomy.

Methods
A retrospective single-centre study was conducted. Demographic data, medical records, intraoperative course, and imaging studies were analysed. The outcome measure was defined by the number of days stayed after the operation. A prolonged length of stay (LOS) stay was defined as a minimum of one additional day beyond the median hospital stay in our patient collective. Bivariate analysis and multiple stepwise regression were used to identify independent factors related to the prolonged hospital stay.

Results
Two hundred consecutive patients who underwent first lumbar microdiscectomy between 2018 and 2022 at our clinic were included in this study. Statistical analysis of factors potentially prolonging postoperative hospital stay was done for a total of 24 factors, seven of them were significantly related to prolonged LOS in bivariate analysis. Sex (p = 0.002, median 5 vs. 4 days for females vs. males) and age (r

s
= 0.35, p ≤ 0.001, N = 200) were identified among the examined demographic factors. Regarding preoperative physical status, preoperative immobility reached statistical significance (p ≤ 0.001, median 5 vs. 4 days). Diabetes mellitus (p = 0.043, median 5 vs. 4 days), anticoagulation and/or antiplatelet agents (p = 0.045, median 5 vs. 4 days), and postoperative narcotic consumption (p ≤ 0.001, median 5 vs. 4 days) as comorbidities were associated with a prolonged hospital stay. Performance of nucleotomy (p = 0.023, median 5 vs. 4 days) was a significant intraoperative factor. After linear stepwise multivariable regression, only preoperative immobility (p ≤ 0.001) was identified as independent risk factors for prolonged length of postoperative hospital stay.

Conclusion
Our study identified preoperative immobility as a significant predictor of prolonged hospital stay, highlighting its value in preoperative assessments and as a tool to pinpoint at-risk patients. Prospective clinical trials with detailed assessment of mobility, including grading, need to be done to verify our results.
A learning-based framework for spatial join processing: estimation, optimization and tuning
Authors: Vu Tin , Belussi Alberto , Migliorini Sara , Eldawy Ahmed 
Published: February 2024

AbstractThe importance and complexity of spatial join operation resulted in the availability of many join algorithms, some of which are tailored for big-data platforms like Hadoop and Spark. The choice among them is not trivial and depends on different factors. This paper proposes the first machine-learning-based framework for spatial join query optimization which can accommodate both the characteristics of spatial datasets and the complexity of the different algorithms. The main challenge is how to develop portable cost models that once trained can be applied to any pair of input datasets, because they are able to extract the important input characteristics, such as data distribution and spatial partitioning, the logic of spatial join algorithms, and the relationship between the two input datasets. The proposed system defines a set of features that can be computed efficiently for the data to catch the intricate aspects of spatial join. Then, it uses these features to train five machine learning models that are used to identify the best spatial join algorithm. The first two are regression models that estimate two important measures of the spatial join performance and they act as the cost model. The third model chooses the best partitioning strategy to use with spatial join. The fourth and fifth models further tune two important parameters, number of partitions and plane-sweep direction, to get the best performance. Experiments on large-scale synthetic and real data show the efficiency of the proposed models over baseline methods.
A collaborative AR application for education: from architecture design to user evaluation
Authors: Masneri Stefano , Domínguez Ana , Pacho Guillermo , Zorrilla Mikel , Larrañaga Mikel , Arruarte Ana 
Published: February 2024

AbstractAugmented reality applications can be used in an educational context to facilitate learning. In particular, augmented reality has been successfully used as a tool to boost students’ engagement and to improve their understanding of complex topics. Despite this, augmented reality usage is still not common in schools and it still offers mostly individual experiences, lacking collaboration capabilities which are of paramount importance in a learning environment. This work presents an application calledARoundTheWorld, a multiplatform augmented reality application for education. It is based on a software architecture, designed with the help of secondary school teachers, that provides interoperability, multi-user support, integration with learning management systems and data analytics capabilities, thus simplifying the development of collaborative augmented reality learning experiences. The application has been tested by 44 students and 3 teachers from 3 different educational institutions to evaluate the usability as well as the impact of collaboration functionalities in the students’ engagement. Qualitative and quantitative results show that the application fulfils all the design objectives identified by teachers as key elements for augmented reality educational applications. Furthermore, the application was positively evaluated by the students and it succeeded in promoting collaborative behaviour. These results show thatARoundTheWorld, and other applications built using the same architecture, could be easily developed and successfully integrated into existing schools curricula.
Special Challenges of Sensitive Images: A HIMSS-SIIM Enterprise Imaging Community Whitepaper
Authors: Towbin Alexander J. , Ding Delaney D. , Eid Moneif , Kimball Heather , Komissarchik Julia , Memarian John , Chadalavada Seetharam C. 
Published: February 2024

AbstractSensitive images represent a new challenge in enterprise imaging. These images, often containing nudity or gruesome content, have the potential to cause emotional harm to patients and people who view the images. Unfortunately, the interoperability standards used in imaging informatics have not yet addressed this issue. Because of this, the software solutions used in healthcare information technology are not able to offer patients and other viewers of image protections. In this Health Information Management Systems Society (HIMSS)/Society for Imaging Informatics in Medicine (SIIM) Enterprise Imaging Community Whitepaper, we define sensitive images, identify unique challenges related to their management, and provide recommendations for future solutions to protect our patients.
Computational Analysis of the Effects of Fiber Deformation on the Microstructure and Permeability of Blood Oxygenator Bundles
Authors: Poletti Gianluca , Ninarello Davide , Pennati Giancarlo 
Published: February 2024

AbstractMechanical loads on the polymeric fibers of oxygenating bundles are commonly present due to bundle press-fitting during device assembly and blood pressure load. However, computational fluid dynamics (CFD) simulations for fiber bundle optimization neglect possible changes in microstructure due to such deformations. The aim of this study is to investigate the impact of fiber deformability on bundle microstructure and fluid dynamics mainly in terms of permeability. Fibers from commercial mats typically used for blood oxygenators were mechanically tested and based on these experimental data, a material model was developed to simulate the structural deformations the fibers undergo under press-fitting and blood pressure loads. Then, CFD simulations were performed on deformed bundle repetitive units to investigate permeability under varying loading conditions. The effects of different bundle geometric parameters on the variation of bundle permeability due to press-fitting were evaluated. Bundle press-fitting results in significant changes in microstructure that are reflected in a bundle permeability more than halved for a 15% press-fitting. This impact on permeability is present in all the simulated fiber bundles and becomes more pronounced as the pitch between fibers and thus bundle porosity decreases. Instead, the analyses on pressurized bundle show only small deformations caused by pressure load, with permeability changes below 1%. While blood pressure effects could be neglected, bundle press-fitting turns out to have a significant impact on bundle microstructure and permeability. Neglecting such microstructure variations during CFD simulations could also lead to incorrect assessment of the local fluid dynamics within the bundle.
“It’s Business”: A Qualitative Study of Moral Injury in Business Settings; Experiences, Outcomes and Protecting and Exacerbating Factors
Authors: Nielsen Karina , Agate Claire , Yarker Joanna , Lewis Rachel 
Published: February 2024

AbstractMoral injury has primarily been studied from a clinical perspective to assess, diagnose and treat the outcomes of morally injurious experiences in healthcare and military settings. Little is known about the lived experiences of those who have had their moral values transgressed in business settings. Public scandals such as Enron suggest that moral injury may also occur in for-profit business settings. In this qualitative study, we examine the lived experiences of 16 employees in for-profit business organisations who identified as having suffered moral injury. Using semi-structured narrative interviews, our findings offer insights into the values that employees feel are transgressed and the pathways between morally injurious experiences and the long-term outcomes. Based on our findings, we propose a conceptual pathway to moral injury, which suggests that experiencing moral transgressions has a profound impact on employees as they feel a threat to their “good-me” identity, however, employees employ various coping strategies to minimise the impact during the event. Employees exited the organisation and often changed career paths to protect themselves from further injury and to make up for moral failure. This study advances our understanding of the experience of moral injury in business settings and the pathway explaining how and why people react differently to moral transgressions.