bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling the intriguing relationship: oncogenic KRAS, morphological shifts, and mutational complexity in pancreatic mucinous cystic neoplasms
Authors: Schulte  , Beck Annika , Marienfeld Ralf , Azoitei Ninel , Barth Thomas , Beutel Alica , Benes Vladimir , Büchler Markus , Gaisa Nadine , Kilani Katja , Giese Nathalia , Michalski Christoph W , Möller Peter , Perkhofer Lukas , Rausch Tobias , Repky Stefan , Roger Elodie , Scheible Jeanette , Seufferlein Thomas , Schirmacher Peter , Berger  , Hackert Thilo , Kleger Alexander 
Published: February 2025

AbstractPancreatic ductal adenocarcinoma (PDAC) often arises from preexisting cystic lesions such as intraductal papillary mucinous neoplasms (IPMN) and mucinous cystic neoplasms (MCN). This study investigated the molecular heterogeneity and mutational landscape of MCN in relation to PDAC, highlighting the significance of KRAS mutations in tumor progression. Utilizing targeted next‐generation sequencing on low‐grade MCN and invasive PDAC samples, we identified a substantial overlap in mutational profiles, particularly mutations in KRAS, TP53, and FBXW7. Specifically, 69.2% of MCN exhibited somatic mutations, with KRAS mutations being a predominant oncogenic driver. The characterization of mutant versus wildtype KRAS variant allele frequencies (VAF) indicated higher mutation levels in PDAC compared to MCN, suggesting an evolutionary trajectory toward malignancy. Further histological analysis of 12 additional MCN cases revealed significant intratumor heterogeneity, with variant KRAS mutation distributions correlating with distinct cellular morphologies and dysplastic features. Additionally, we explored the potential of liquid biopsies, demonstrating a concordance rate of 71.4% for KRAS mutation detection in circulating tumor DNA (ctDNA) relative to tissue biopsies across cohorts. Our findings underscore the relevance of evaluating KRAS mutations—herein referred to as VAF per microdissected region—as they relate to histopathological markers of dysplasia, contributing to improved stratification of pancreatic lesions and facilitating personalized treatment strategies. In conclusion, this comprehensive analysis of MCN highlights the importance of KRAS as a crucial biomarker for both malignant progression and therapeutic decision‐making in pancreatic pathology. Ultimately, our study suggests that characterizing the mutational landscape and histological features of MCN can enhance early detection and intervention strategies for at‐risk patients. © 2025 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy for Pediatric Abdominal Rhabdomyosarcoma: Experience of a Reference Pediatric Oncosurgical Center
Authors: Urla Cristian , Wagner Benedikt , Schmidt Andreas , Fideler Frank , Schäfer Jürgen , Neunhoeffer Felix , Ebinger Martin , Seitz Guido , Fuchs Jörg 
Published: February 2025

ABSTRACTBackgroundPediatric abdominal rhabdomyosarcoma (RMS) represents an oncosurgical challenge. For adults with peritoneal carcinomatosis and for children/adolescents with desmoplastic small round cell tumors (DSRCT), cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) has been shown to be an effective treatment. Thereby, we present the largest series on CRS and HIPEC in patients with abdominal RMS.Patients and MethodsBetween August 2013 and January 2024, 18 patients (9 female and 9 male) with abdominal RMS underwent CRS and HIPEC at our institution. All patients were treated according to an institutionalized protocol. The indication for surgery was established by a multidisciplinary national tumor board. HIPEC was performed using cisplatin (37.5–100 mg/m2) and doxorubicin (15–30 mg/m2) for 60 min at 42°C. A retrospective review of patient's records was performed.ResultsThe median age at operation was 3.8 years. A complete cytoreduction was achieved in 17/18 patients. Median length of hospital stay was 11 days. Mean length of ICU stay was 2.5 days. No procedure‐associated complications and no major short‐/long‐term toxicities were recorded. The median follow‐up was 20 months. The 5‐year overall survival (OS) and event‐free survival (EFS) were 72% and 53%, respectively. Patients >10 years and those with alveolar histology had the worst prognosis (OS 0%). Patients with relapse after HIPEC (6/18) had a significantly poorer survival compared with those without recurrence (OS 33% vs. 100%, p = 0.001).ConclusionsCRS and HIPEC are effective in patients with abdominal RMS. Patients >10 years, those with alveolar RMS and those with relapse after HIPEC had the worst outcome.
Image‐Guided Biopsy for the Diagnosis and Molecular Profiling of Hepatoblastoma
Authors: Schoeman Sean , Escobar Fernando , Kreiger Portia , MacFarland Suzanne , Mattei Peter , Rungsiprakarn Phassawan , Srinivasan Abhay , Acord Michael 
Published: February 2025

ABSTRACTIntroductionStudies on the use of image‐guided percutaneous biopsy for hepatoblastoma (HB), and recommendations put forth by the pediatric hepatic international tumor trial (PHITT), are limited. It is unknown if sufficient tissue can be obtained for trial enrollment as well as molecular profiling, which will likely play a key role in informing future treatment strategies.Methods and MaterialsPatients with HB who underwent percutaneous biopsy at initial diagnosis in interventional radiology (IR) over a 12‐year period at a single center were included. Patient demographics, pretreatment extent of disease (PRETEXT) stage, tumor size, and procedure details were collected. Pathology reports and tumor genomic analysis, when performed, were assessed for specimen adequacy. Post‐procedure records were assessed for hemoperitoneum.ResultsA total of 33 percutaneous biopsies were performed on 32 patients [17 female; median age 1.3 years (IQR: 0.7–2.5 years); median weight 10.5 kg (IQR: 7.4–12.7 kg)]. Most (n = 27) had a single liver lesion, and most (n = 18) were PRETEXT II. A total of 15 were positive for at least one annotation factor. Median longest tumor axis was 9.3 cm (IQR: 5.0–13.5 cm). A total of 16 patients had concurrent non‐targeted liver biopsy, per PHITT recommendations. An 18‐gauge instrument was most commonly used (n = 24, 73%) with a median of 8 cores (IQR: 6–12) obtained. There were no instances of hemoperitoneum.Tissue was adequate for histologic diagnosis in 97% (n = 32), with histologic subtyping obtained in 94% (30/32). When available (n = 29), comparison with the subsequent surgical resection specimen showed subtype concordance in 15 (52%) patients and minor variations secondary to sampling or treatment effect in 14 patients. Molecular profiling was completed on 21/21 specimens (100%), with 19/21 (90%) showing potentially clinically significant variants, most commonly in CTNNB1 (16/21).ConclusionIn this single‐center study, percutaneous biopsy resulted in no serious adverse events, a high rate of diagnosis, and successful subtyping and molecular characterization of HB.
Differential Expression of BCL2 and IGFBP2 in Childhood Immune Thrombocytopenic Purpura Clinical Subtypes: Implications for Predicting Disease Progression and Apoptotic Regulation
Authors: Bakr Salwa , Dash Hanaa El , Youssef Asmaa Ahmed Ali , El‐Hamid Rehab Galal Abd 
Published: February 2025

ABSTRACTBackgroundImmune thrombocytopenic purpura (ITP), which poses challenges in treatment response, is an autoimmune‐mediated bleeding disorder with an extremely complex pathogenesis and unpredictable clinical progression. Dysregulation of apoptotic pathways may influence both the pathogenesis and prognosis of ITP. This study aimed to evaluate the expression patterns of the apoptotic protein insulin‐like growth factor‐binding protein 2 (IGFBP2) and the anti‐apoptotic protein B‐cell lymphoma 2 (BCL2) as potential predictive or prognostic biomarkers for disease progression in childhood ITP.Patients and MethodsThe expression levels of BCL2 and IGFBP2 were assessed in peripheral blood samples from 40 pediatric ITP patients and 30 age‐ and sex‐matched healthy controls using enzyme‐linked immunosorbent assays.ResultsPlasma levels of BCL2 and IGFBP2 were higher in ITP patients than in control subjects. Although the difference in IGFBP2 expression was not statistically significant (p = 0.910), BCL2 expression was significantly elevated (p < 0.001). Notably, chronic ITP patients had significantly lower levels of both IGFBP2 and BCL2 markers compared to patients who achieved spontaneous recovery (p < 0.001).ConclusionBCL2 and IGFBP2 appear to be promising noninvasive biomarkers for predicting disease outcomes in newly diagnosed ITP, emphasizing the need for validation in large‐scale, multicenter longitudinal studies.
Evaluation of the physical–chemical properties of the blend of arrowroot starch and cactus mucilage
Authors: de Lima Silva Ivo Diego , Caetano Viviane Fonseca , de Andrade Michelle Félix , de Moraes Filho Luiz Emílio Pessoa Timeni , de Almeida Yeda Medeiros Bastos , do Nascimento Simões Adriano , Brito Andréa Monteiro Santana Silva , Maria Vinhas Glória 
Published: February 2025

AbstractThe replacement of these polymers by biodegradable ones is a promising alternative to mitigate the environmental problems caused by these materials. The mixture of biopolymers obtained from arrowroot starch (ST) and cactus mucilage (MU) may have its properties enhanced for the production of food packaging. In this study, it was used solution casting technique to develop blends of ST and MU in different compositions, analyzing their thermal, mechanical, and physical–chemical properties. Through a principal component analysis, it was verified that the technique used to mix the polymers was satisfactory. A reduction in the crystallinity of the polymer blends was noted in all formulations, especially when compared to films made only from arrowroot starch or cactus mucilage. This alteration means that the materials have lower stiffness. Water vapor permeability was shown to be lower in the blend with the highest starch concentration. The films with a 75/25 ST/MU formulation exhibited higher elongation at break values, while no significant changes in tensile strength were observed when compared to other concentrations, except for the pure ST films, which showed lower tensile strength and greater elongation at break. For the thermogravimetric analysis, comparing between the blends, an increase in thermal stability was perceived for the formulation ST/MU 75/25. Based on these results, the evaluated blends show potential for use as food packaging in short‐term applications.Highlights
Development of arrowroot starch blends and cactus mucilage.
Polymer blends showed lower crystallinity values.
Increased starch concentration reduced water vapor permeability.
The ST/MU 75/25 blend had better tensile strength and specific deformation.
Starch addition improved thermal stability in blend formulations.
Conjugated polymer‐reinforced cellulosic frameworks: a promising approach for flexible electronics
Authors: Thapa Yub Narayan , Rajchal Bimal , Karki Deepshikha , Tamang Sonam , Staudinger Ulrike , Kafle Bhim Prasad , Adhikari Rameshwar 
Published: February 2025

AbstractFlexible electronics are seamlessly integrated into our lives, from foldable displays to smart wearables, redefining our interaction with technology. The use of metals and semiconductors in these flexible devices is limited due to their poor bendability and stretchability. Consequently, integrating conjugated polymers (CPs) into a cellulosic framework has emerged as a promising approach for advancing flexible electronics. Cellulose, as an abundant and sustainable biopolymer, offers a compelling solution to the escalating global e‐waste crisis by providing a cost‐effective and biodegradable substrate. This synergy has the potential to address environmental concerns and unlock new avenues of flexible, eco‐friendly and sustainable electronic devices. Herein the unique properties and synthesis routes of CPs are briefly introduced including their opportunities and challenges. The review discusses a facile and efficient approach to circumvent the challenges of CPs using cellulose as a substrate. The review explores fabrication approaches of CP–cellulose composites aimed at enhancing mechanical, electrical and optical properties. Through a critical examination of recent studies and advancements, it highlights how CPs reinforce the cellulose framework and investigates their structure–property relationships, which are crucial for tailoring the properties for desired applications. Finally, the review presents an outlook on potential challenges and prospects for advancing CP‐based cellulose composites in flexible electronics. © 2025 Society of Chemical Industry.
How to Alleviate Students' Academic Burnout? Using Latent Profile Analysis and Network Analysis to Explore the Positive Impact of School Climate and Achievement Goal Orientation
Authors: Xue Shengping , Lu Aitao , Chen Wanyi , Liang Yueer , Li Xiaoya , Liu Wantang , Zhu Yanxuan , Wang Xiating , Zeng Shan 
Published: February 2025

ABSTRACTAcademic burnout, triggered by a combination of external factors and internal psychological characteristics, significantly impacts secondary school students' academic performance and psychological well‐being. We use achievement goal orientations, including mastery approach, mastery avoidance, performance approach, and performance avoidance, to identify distinct motivational profiles, in 1137 middle school students. Subsequently, we used regression mixed models and network analysis to examine between‐subgroup differences in the relationship between school climate, an external factor, and academic burnout. Latent profile analysis (LPA) revealed three typical patterns of goal pursuit: success‐oriented (17.8%), characterized by motivation across various goals; moderated motivation (70%), lacking a salient dominant goal and clear motivational tendency; and unmotivated (12.8%), showing a lack of interest and motivation in learning. Among the success‐oriented subgroup, academic burnout was most strongly associated with campus climate. Network comparison results showed stronger links between “student‐student support” and “exhaustion,” as well as “autonomy” and “efficacy reduction,” among students in the success‐oriented subgroup compared to those in the unmotivated subgroup. Therefore, educational or counseling programs should be developed, tailored to different goal‐seeking models and the specific school climate.
Neutrinoloser Doppelbetazerfall: Der Gral der Neutrinophysik
Authors: Schwingenheuer Bernhard 
Published: February 2025

ZusammenfassungNeutrinos sind besondere Teilchen, deren Eigenschaften uns viel über das Standardmodell der Teilchenphysik und die Geschichte des Universums sagen können. Auch nach siebzig Jahren sind wichtige Eigenschaften wie ihre Masse und Natur ungeklärt. So ist es noch eine offene Frage, ob sie Majorana‐Teilchen sind oder nicht. Zu den vier bisherigen Nobelpreisen zur Neutrinoforschung werden vermutlich noch weitere hinzukommen.
The Anticonvulsant Effect of Nonsteroidal Anti‐Inflammatory Drug, Fenoprofen, in Pentylenetetrazole‐Induced Epileptic Rats: Behavioral, Histological, and Biochemical Evidence
Authors: Rahimi‐Tesiye Maryam , Rajabi‐Maham Hassan , Azizi Vahid , Hosseini Abdolkarim 
Published: February 2025

ABSTRACTThis study aimed to evaluate the anticonvulsant properties of fenoprofen on the experimental model of pentylenetetrazole (PTZ)‐induced epilepsy. Male Wistar rats were randomly grouped into five, and the kindling model was induced by intraperitoneal injection of PTZ 35 (mg/kg) every other day for 1 month. Aside from the control and PTZ groups, three groups received intraperitoneal injections of fenoprofen at doses of 10, 20, and 40 (mg/kg) before each PTZ injection. Rats were challenged with PTZ 70 (mg/kg) 1 week after kindling development. Then rats were subjected to deep anesthesia, and serum and brain samples were prepared. Oxidative stress (OS) markers (malondialdehyde, superoxide dismutase, and glutathione peroxidase) were measured in serum samples. Hippocampal tissue was used to investigate the relative expression of OS‐related genes (nuclear factor [erythroid‐derived 2]‐like 2 (Nrf2)/heme oxygenase 1 (Hmox1)) and histological studies. Seizure behavior was assessed based on Lüttjohann's score. In treated groups, the number of myoclonic jerks and generalized tonic–clonic seizure (GTCS) duration decreased significantly, while myoclonic jerks and GTCS latency increased compared with the PTZ group. The biochemical evaluation revealed the antioxidative effects of fenoprofen. The decreased expression of Nrf2/HO‐1 genes in the PTZ group was reversed after fenoprofen administration. The results of the histological study obtained from Nissl staining in the hippocampal tissue also confirmed the protective effect of fenoprofen. The anticonvulsant effects of fenoprofen seem to be through inhibition of OS‐related markers, induction of protective effect in hippocampal tissue, and activation of the Nrf2/HO‐1 signaling pathway.
Novel phytotoxic compounds from Streptomyces sp. Caat 7–52: a potential bioherbicide against Conyza canadensis
Authors: Junior Osvaldo Luiz Ferreira , Oliveira Raquel Dias , Ferreira Henrique RGM , Boni Eduardo Defendi , Melo Itamar Soares , Moraes Luiz Alberto Beraldo , Souza Danilo Tosta 
Published: February 2025

AbstractBACKGROUNDThe rise of herbicide‐resistant weeds like Conyza canadensis L. poses a challenge to modern agriculture, driving the need for eco‐friendly alternatives. Microbial metabolites from actinobacteria species offer promising weed‐control solutions. This study aims to screen and identify an actinobacteria isolate from Brazil's Caatinga biome that produces phytotoxic metabolites and to characterize its compounds.RESULTSAn isolate, named as Caat 7–52, was selected because of its significant phytotoxic effects against Lemna minor L. Phylogenetic analyses using six concatenated genes (gyrB, recA, rpoB, trpB, atpD and 16S rRNA) confirmed Caat 7–52's close relationship to Streptomyces musisoli TBRC 9950T, despite phenotypical differences. Bioassay‐directed isolation against L. minor revealed 3‐hydroxybenzoic acid and albocycline as phytotoxins, with minimum inhibitory concentrations of 50.00 and 3.12 μg mL−1, respectively. Albocycline analogues were also detected and exhibited moderate phytotoxicity in L. minor. In addition, albocycline effectively inhibited the seed germination of C. canadensis with a minimum inhibitory concentration of 6.25 μg mL−1, marking the first report of albocycline's phytotoxic activity. Direct use of the fermented broth selectively inhibited dicot weeds, offering a sustainable and solvent‐free weed management strategy.CONCLUSIONThe discovery of Streptomyces sp. Caat 7–52 and its metabolites, combined with the direct application of fermented broth, represents a significant advancement in sustainable weed control. This bioherbicidal approach offers an environmentally friendly alternative for managing resistant weeds like C. canadensis and supports the broader use of microbial metabolites in integrated pest management programs. © 2025 Society of Chemical Industry.
Development of recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid and simple detection of Tylenchulus semipenetrans in soil
Authors: Song Zhiqiang , Cheng Yi , Wang Tuhong , Wang Qipei , Qiu Caisheng , Qiu Huajiao 
Published: February 2025

AbstractBACKGROUNDTylenchulus semipenetrans, the causal agent of citrus slow decline disease, is one of the most destructive plant‐parasitic nematodes in all citrus‐growing regions of the world, causing significant reductions in citrus growth and yield. Accurate and rapid detection of T. semipenetrans is critical for the diagnosis and effective control of the disease.RESULTSWe developed a rapid, visual, isothermal detection method using recombinase polymerase amplification combined with a lateral flow dipstick (RPA‐LFD) assay to detect T. semipenetrans in soil. The primers and a probe were designed based on sequence differences in the internal transcribed spacer region 1 (ITS1) of ribosomal DNA (rDNA) among T. semipenetrans and four other Tylenchulus species. The RPA reaction can be performed in 10–25 min at a constant temperature ranging from 30 to 45 °C, and the result can be read directly on the LFD within 3 min. Under the optimized conditions, the RPA‐LFD assay could specifically detect T. semipenetrans with a sensitivity as low as 10−2 second‐stage juveniles/0.5 g soil, which was 10‐fold more sensitive than that of the conventional PCR assay. Furthermore, we combined a soil DNA extraction method with the RPA‐LFD assay to achieve simple and rapid detection of T. semipenetrans in natural field soil samples within 1 h.CONCLUSIONThe developed RPA‐LFD assay is a simple, rapid, specific, sensitive and visual method for detecting T. semipenetrans. It shows great potential for on‐site rapid detection applications, especially in resource‐limited settings. © 2025 Society of Chemical Industry.
Assessment of Phage‐Displayed Peptides Targeting Cancer Cell Surface Proteins: A Comprehensive Molecular Docking Study
Authors: Quilumba‐Dutan Verónica , Carreón‐Álvarez Clara , Sanabria‐Ayala Víctor , Hidalgo‐Figueroa Sergio , Chakraborty Swaroop , Valsami‐Jones Eugenia , López‐Revilla Rubén , Rodríguez‐López José Luis 
Published: February 2025

ABSTRACTPeptides binding overexpressed breast and cervical cancer cell surface proteins can be isolated by phage display technology, and their affinity to their potential receptors can be assessed by molecular docking. We isolated 44 phage clones displaying dodecapeptides with high affinity to HeLa cervical cancer and MDA‐MB‐231 (MDA) breast cancer cells by repeated biopanning of an MK13 phage library and explored their affinity to specific proteins by molecular docking. Six peptides appeared repeatedly during biopanning: two with affinity to HeLa (H5/H21), and four with affinity to MDA cells (M3/M7/M15/M17). Peptide pairs M3/H5 and H1/M17 had affinity to both cell lines. A systematic review identified Annexin A2, EGFR, CD44, CD146, and Integrin alpha V as potential protein targets in HeLa cells, and Vimentin, Galectin‐1, and Annexins A1 and A5 in MDA cells. Via virtual screening, we selected six peptides with the highest total docking scores: H1 (−916.32), H6 (−979.21), H19 (−1093.24), M6 (−732.21), M16 (−745.5), and M19 (−739.64), and identified that docking scores were strengthened by the protein type, the interacting amino acid side chains, and the polarity of peptides. This approach facilitates the selection of relevant peptides that could be further explored for active targeting in cancer diagnosis and treatment.
Nondestructive TVB‐N Detection in Packaged Beef Using Hyperspectral Imaging
Authors: Zhang Wenxiang , Pan Liao , Cheng Xueyu , Lu Lixin 
Published: February 2025

ABSTRACTHyperspectral imaging (HSI) technology has shown potential in monitoring fresh meat. It is necessary to investigate the rapid detection technology of packaged fresh meat in order to ensure food quality and safety. In this study, hyperspectral images of beef in the presence of packaging film were collected, and the spectra were optimized using spectral pretreatment, band selection and film radiation correction methods to predict the total volatile basic nitrogen (TVB‐N) of beef in the presence of packaging film. The results of the competitive adaptive reweighted sampling (CARS) combined with least squares support vector machines (CARS‐LSSVM) model constructed by using the corrected reflectance spectra were  = 0.8687, RMSEP = 3.1970 mg/100 g, which is higher than that obtained before the correction ( was 0.8168, and RMSEP was 3.0449 mg/100 g). The results reveal that HSI with radiation correction has the potential in monitoring the TVB‐N content of beef through packaging film.