bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic factors of chronic graft‐versus‐host disease after allogeneic blood stem‐cell transplantation
Authors: Pavletic Steven Z. , Smith Lynette M. , Bishop Michael R. , Lynch James C. , Tarantolo Stefano R. , Vose Julie M. , Bierman Philip J. , Hadi Abdul , Armitage James O. , Kessinger Anne 
Published: March 2005

AbstractAllogeneic hematopoietic stem cells in peripheral blood transplantation (alloPBSCT) or bone marrow transplantation (alloBMT) have different biological characteristics which may affect differently prognostic factors for incidence and severity of chronic graft‐versus‐host disease (cGVHD). To determine the prognostic factors of cGVHD in patients receiving alloPBSCT, data on 87 patients who survived at least 100 days after matched related donor myeloablative transplantation were analyzed. Factors significantly associated with higher incidence of cGVHD after alloPBSCT included CMV‐positive donor, acute skin GVHD, and diagnoses other than lymphoma. Factors predictive for poor survival following cGVHD diagnosis included platelet count < 100,000/mm3 and history of acute liver GVHD. Acute liver GVHD and etoposide in the preparative regimen significantly increased risk of death due to cGVHD after alloPBSCT. All alloPBSCT multivariate models were fit to an independent cohort of comparable matched related donor alloBMT patients (n = 75). After alloBMT, only acute skin GVHD and diagnoses other than lymphoma retained prognostic significance for predicting cGVHD. Low platelet count was the only variable predictive for poor survival in cGVHD patients after alloBMT. Acute liver GVHD was the only factor that retained prognostic significance for risk of death due to cGVHD after alloBMT. These data suggest there are some cGVHD prognostic factors that may be unique to recipients of alloPBSCT. More studies are needed to determine whether cGVHD prognostic systems should be used interchangeably in patient populations receiving different stem‐cell products. Am. J. Hematol. 78:265–274, 2005. © 2005 Wiley‐Liss, Inc.
Successful treatment of Diamond‐Blackfan anemia with metoclopramide
Authors: Akiyama Masaharu , Yanagisawa Takaaki , Yuza Yuki , Yokoi Kentaro , Ariga Masamichi , Fujisawa Kohji , Hoshi Yasutaka , Eto Yoshikatsu 
Published: March 2005

AbstractDiamond‐Blackfan anemia (DBA) is a congenital anemia characterized by a low reticulocyte count, the absence or severe reduction of hemoglobin‐containing cells in the bone marrow, and normal megakaryocytic and granulocytic differentiation. Although the anemia may initially respond to corticosteroid therapy, many patients require lifelong red blood cell (RBC) transfusion, leading to infectious complications and iron overload. Metoclopramide has recently been used to treat DBA. Treatment with metoclopramide induces the release of prolactin from the pituitary and stimulates erythropoiesis. For these reasons, we used metoclopramide to treat a 20‐year‐old man with DBA refractory to low and high doses of corticosteroids, cyclosporin A, and tacrolimus (FK506). The hemoglobin and hematocrit slowly increased, and he has remained asymptomatic and transfusion‐independent for 8 months. Metoclopramide therapy should be considered in patients with refractory DBA before treatment‐related complications develop. Am. J. Hematol. 78:295–298, 2005. © 2005 Wiley‐Liss, Inc.
T‐cell large granular lymphocytic leukemia and hereditary hemochromatosis: A fortuitous association?
Authors: Gaur S. , Mansoor S. , Aish L. 
Published: March 2005

AbstractWe describe a patient with hereditary hemochromatosis (homozygous for C282Y mutation) and neutropenia who was found to have underlying T‐cell large granular lymphocytic (T‐LGL) leukemia. The diagnosis was confirmed by demonstrating T‐cell receptor (TCR) gene rearrangement by polymerase chain reaction (PCR). Multiple quantitative and qualitative defects have been described for the T cells of patients with hemochromatosis. Although the association between the two may be fortuitous, this case report raises the possibility that the T cells in these patients may be susceptible to leukemic transformation as well. Am. J. Hematol. 78:299–301, 2005. © 2005 Wiley‐Liss, Inc.
Ubiquitination of red blood cell α‐spectrin does not affect heterodimer formation
Authors: Riahi Mahnoush H. , Kakhniashvili David G. , Goodman Steven R. 
Published: March 2005

AbstractErythrocyte α‐spectrin is ubiquitinated in repeats α20/α21, which also represents the nucleation site for contact with the β subunit which leads to heterodimer formation by a zippering mechanism. In this study we have determined the second‐order rate constant for association of ubiquitinated α′‐spectrin, nonubiquitinated α‐spectrin, and β‐spectrin into the α′β or αβ heterodimer. The rate constant for incorporation of monomers into heterodimers at 37°C were (5.181 ± 0.001) × 105 M−1 sec−1 for total α‐spectrin (α + α′), (5.121 ± 0.001) × 105 M−1 sec−1 for α′‐spectrin, and (5.178 ± 0.003) × 105 M−1 sec−1 for β‐spectrin. We conclude that ubiquitination of α‐spectrin does not regulate heterodimer formation. Am. J. Hematol. 78:281–287, 2005. © 2005 Wiley‐Liss, Inc.
Efficacy of rituximab in an aggressive form of multicentric Castleman disease associated with immune phenomena
Authors: Ocio Enrique M. , Sanchez‐Guijo Fermin M. , Diez‐Campelo Maria , Castilla Cristina , Blanco Oscar J. , Caballero Dolores , San Miguel Jesus F. 
Published: March 2005

AbstractMulticentric Castleman disease (MCD) is an uncommon lymphoproliferative disorder for which the best therapeutic option is not yet well established. Immune‐related disorders are rare complications of MCD. We report on an MCD case in a 23‐year‐old patient with extensive abdominal involvement and associated immune hemolytic anemia and Raynaud phenomenon. He was negative for human immunodeficiency virus (HIV) and human herpesvirus‐8 (HHV‐8). After 8 courses of the anti‐CD20 monoclonal antibody (rituximab), the patient achieved complete remission. Interestingly, Raynaud phenomenon disappeared under treatment and no new hemolytic events occurred. Anti‐CD20 antibody treatment could be an attractive therapeutic approach for MCD, mainly when immune‐related disorders are associated. Am. J. Hematol. 78:302–305, 2005. © 2005 Wiley‐Liss, Inc.
Hydroxychloroquine causes severe vacuolar myopathy in a patient with chronic graft‐versus‐host disease
Authors: Bolaños‐Meade Javier , Zhou Lan , Hoke Ahmet , Corse Andrea , Vogelsang Georgia , Wagner Kathryn R. 
Published: March 2005

AbstractA 51‐year‐old man developed progressive debilitating limb and respiratory muscle weakness while undergoing treatment for chronic graft‐versus‐host disease secondary to allogeneic bone marrow transplant for mantle cell lymphoma. He had a normal serum creatine kinase level and acetylcholine receptor antibodies were negative. Electromyography showed a severe, nonirritable myopathy and a sensory motor axonal polyneuropathy. A muscle biopsy showed a necrotizing, vacuolar myopathy with many fibers containing autophagic and red‐rimmed vacuoles, suggestive of an amphiphilic drug myopathy. The patient's strength and function improved significantly after discontinuation of hydroxychloroquine. Am. J. Hematol. 78:306–309, 2005. © 2005 Wiley‐Liss, Inc.
Dendritic cell counts in the peripheral blood of healthy adults
Authors: Haller Hasskamp Joanne , Zapas John Louis , Elias Elias George 
Published: March 2005

AbstractThe normal range for adult blood dendritic cells (DC) has not been established. Blood DC counts were assayed directly from blood for 16 female and 11 male healthy adults with an age range of 22–60 years old (median, 46 years). DC were defined as lineage 1‐negative/dim, CD34‐negative/dim, and HLA‐DR‐positive within the total peripheral blood leukocyte (PBL) population. Impedance counter complete blood counts were used with flow cytometry percentages to calculate the absolute DC count and DC percentage of mononuclear cells (MNC). The normal adult ranges for DC calculated as the mean ± 2SD were 0.16–0.68% PBL, 0.55–1.63% MNC, and 13–37 DC/μL of blood. Am. J. Hematol. 78:314–315, 2005. © 2005 Wiley‐Liss, Inc.
Primary mediastinal B‐cell lymphoma with sclerosis: Report of 11 cases treated with intensified‐CHOP plus radiotherapy
Authors: Siracusano Licia , Balzarotti Monica , Magagnoli Massimo , Castagna Luca , Rahal Daoud , Santoro Armando 
Published: March 2005

AbstractPrimary mediastinal B‐cell lymphoma (PMBCL) is a clinicopathological entity with aggressive behavior. Retrospective evaluation suggests the need for intensive chemotherapy programs. From 1997 to February 2003, a total of 11 cases of previously untreated PMBCL with sclerosis were treated at our institution with 5 courses of intensified CHOP (ICHOP) regimen and mediastinal irradiation. Three patients were submitted to high‐dose chemotherapy with peripheral‐blood stem‐cell support, followed by radiotherapy, because of intermediate‐risk age‐adjusted International Prognostic Index at diagnosis. After a median follow up of 30 months, all patients but one are in continuous complete remission (CR), and overall survival is 100%. Am. J. Hematol. 78:312–313, 2005. © 2005 Wiley‐Liss, Inc.
Design, synthesis, and application of azabicyclo[X.Y.0]alkanone amino acids as constrained dipeptide surrogates and peptide mimics
Authors: Cluzeau Jérôme , Lubell William D. 
Published: March 2005

AbstractAzabicyclo[X.Y.0]alkanone amino acids are challenging synthetic targets and useful tools for studying structure–activity relationships of native peptide ligands. They have been employed to increase potency and stability in conformationally rigid enzyme inhibitors and receptor ligands. Since last reviewed in 1997, activity in their synthesis and application has increased significantly and access is now available to a wider diversity of these peptide mimics. This review focuses on recent syntheses of these heterocyclic amino acids and their application in the investigation of biologically active peptides and peptide mimics. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2005
Erratum: Crystal structures of collagen model peptides with Pro‐Hyp‐Gly repeating sequence at 1.26 Å resolution: Implications for proline ring puckering, Kenji Okuyama, Chizuru Hongo, Rie Fukushima, Guanghan Wu, Hirotaka Narita, Keiichi Noguchi, Yuji Tanaka, Norikazu Nishino, Biopolymers (Peptide Science)(2004)76(5) 367–377
Authors: Okuyama Kenji , Hongo Chizuru , Fukushima Rie , Wu Guanghan , Narita Hirotaka , Noguchi Keiichi , Tanaka Yuji , Nishino Norikazu 
Published: March 2005

AbstractThe original article to which this Erratum refers was published in Biopolymers (Peptide Science) (2004) 76(5) 367–377
Underlying mechanisms of pronociceptive consequences of prolonged morphine exposure
Authors: Ossipov Michael H. , Lai Josephine , King Tamara , Vanderah Todd W. , Porreca Frank 
Published: March 2005

AbstractThe opioid analgesics, commonly exemplified by morphine, represent the best option for the treatment of severe pain and for the management of chronic pain states, of both malignant and nonmalignant origin. It is well recognized that the prolonged use of opioids is associated with a requirement for ever‐increasing doses in order to maintain pain relief at an acceptable and consistent level. This phenomenon is termed analgesic tolerance. While the concept that tolerance can develop as a result of cellular adaptations to the presence of the opioid has been proposed, it is now becoming abundantly clear that tolerance may also be related to a state of hyperalgesia that results from exposure to the opioid itself. Patients who receive long‐term opioid therapy sometimes develop unexpected, abnormal pain. Similar paradoxical opioid‐induced pain has been confirmed in a number of animal studies, even during the period of continuous opioid delivery. A number of recent studies have demonstrated that such pain may be secondary to neuroplastic changes that occur in the brain and spinal cord. One such change may be the activation of descending pain facilitation mechanisms arising from the rostral ventromedial medulla (RVM) elicited in part by increased activity of cholecystokinin (CCK) in the RVM. A cascade of pronociceptive events may follow, such as opioid‐induced upregulation of spinal dynorphin levels that promotes enhanced input from primary afferent nociceptors. This mechanism appears to depend on intact descending pathways from the RVM, since interrupting this pathway abolishes enhanced abnormal pain. Furthermore, extended opioid exposure also can elicit increased calcitonin gene related peptide (CGRP) and substance P expression in the dorsal root ganglia. It is probable that increased pain elicited by opioids is a critical factor in the behavioral manifestation of opioid tolerance because the same manipulations that block abnormal pain also block antinociceptive tolerance. Taken together, such studies show that opioids elicit systems‐level adaptations resulting in pain due to descending facilitation, upregulation of spinal dynorphin, and enhanced, evoked release of excitatory transmitters from primary afferents. These adaptive changes in response to sustained exposure to opioids indicate the need for the evaluation of the clinical consequences of long‐term opioid administration. Additionally, these findings suggest a need for novel chemistry involving design of agents that may counteract opiate‐induced neuroplastic adaptations resulting in pain relief without analgesic tolerance. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2005
Structural features of a central mismatch in oligonucleotide hybrid duplexes visualized via Raman spectroscopy: Model system for evaluation of potential “antisense” drugs
Authors: Nĕmec̆ek Daniel , Vaisocherová Hana , S̆tĕpánek Josef , Turpin Pierre‐Yves 
Published: April 2005

AbstractStructural features of mismatched base pairs were studied on four nonamer hybrid duplexes formed between the 5′‐d(GTGATATGC)‐3′ complement and its 5′‐r(GCAUNUCAC)‐3′ (N = A, C, G, U) counterparts. This oligonucleotide set is considered a model molecular system for future systematic studies of various modifications of internucleotide linkages with respect to their impact on the structure of mismatched base pairs. Raman spectra, measured at 15°C, revealed the prevailing A‐like structure of the RNA strand and mixed A‐like and B‐like characteristics for the DNA strand. All three mismatches disturb only weakly the overall conformation of the hybrid duplex in contrast to analogous mismatched DNA duplexes. In particular, the dT · rG mismatch influences the global hybrid duplex geometry almost negligibly. The dT · rC and dT · rU mismatches induce somewhat more pronounced distortions of the backbone structure and of the thymine position, the latter being expressed by a change of the surrounding methylene group without effect on the carbonyl's vibrations. Structural effects of the mismatches correlate well with the duplex thermodynamic stabilities obtained by ultraviolet (UV) absorption, i.e., the dT · rG mismatch decreases the hybrid duplex stability very weakly while the effect of both pyrimidine–pyrimidine mismatches is considerable. © 2005 Wiley Periodicals, Inc. Biopolymers 79: 1–8, 2005This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Phosphorylcholine is favorable for antibody production from hybridoma cells
Authors: Montano Ximena , Lewis Andrew L. , Leppard Simon W. , Lichtenstein Conrad 
Published: April 2005

AbstractGrowth of antibody‐secreting hybridomas requires special conditions such as serum‐free defined media containing growth factors and vitamins. However, the surface on which these cells can proliferate has been shown to play an important role. Phosphorylcholine (PC)‐based polymers are zwitterionic compounds with nonbiofouling properties. These polymers are characterized by having reduced protein absorption properties. Our aim was to determine whether well‐established hybridoma cell lines were able to proliferate and produce measurable amounts of monoclonal antibodies when grown on PC‐polymer‐coated surfaces. Comparative experiments using four well‐known hybridoma cell lines (PAb421, PAb246, PAb1801 which recognize p53, and PAb280 which recognizes SV40 small t antigen) grown on PC‐polymer‐coated, uncoated, and two commercially available tissue culture plates showed that PC‐polymer‐coated plates were more efficient than uncoated plates in sustaining cell growth and monoclonal antibody production/secretion as defined by growth assays and ELISA. Also, results demonstrated that PC‐polymer‐coated plates were able to perform better than commercially available plates. These observations suggest that PC polymers could be used as an alternative, efficient surface coating to grow hybridoma cell lines and allow detectable antibody secretion. © 2005 Wiley Periodicals, Inc.
Isomeric Mono‐ and Bis[(phosphane)gold(I)] Thiocyanate Complexes
Authors: Berger Raphael J. F. , Patzschke Michael , Schneider Daniel , Schmidbaur Hubert , Sundholm Dage 
Published: April 2005

AbstractThe solid‐state IR spectrum of Me3PAuSCN shows two signals in the range of the C–N stretching vibrations at 2075 and 2113 cm−1. On the basis of thoroughly tested quantum chemical ab initio calculations (MP2 level of theory) these signals have been assigned to the two isomeric forms Me3PAuNCS and Me3PAuSCN. The molecular structures, the vibrational frequencies, and the relative energies of the two species have been calculated and the results compared with the experimental IR data. Treatment of Me3PAuSCN with equimolar quantities of [(Me3P)Au]+[SbF6]− in CH2Cl2 at −78 °C gives the dinuclear reaction product [C7H9Au2NP2S]+[SbF6]− in high yields. A comparison of results of ab initio calculations and IR data suggest that at least three isomeric cationic species [(R3PAu)2NCS]+, [(R3PAu)2SCN]+ and [(R3PAu)SCN(AuPR3)]+ are present, the second and third being the predominant components. The structures and vibrational frequencies of all three species have been calculated. The relative energies in the gas phase and in solution are discussed and compared with the corresponding data of the experimental IR spectra.
Marriage and ethnicity predict treatment in localized prostate carcinoma
Authors: Denberg Thomas D. , Beaty Brenda L. , Kim Fernando J. , Steiner John F. 
Published: March 2005

AbstractBACKGROUNDPrimary treatment for early‐stage prostate carcinoma includes expectant management or, for curative intent, radical prostatectomy or radiotherapy. Treatment recommendations are generally guided by clinical factors such as Gleason grade, prostate‐specific antigen level, comorbid illnesses, and patient age. Sociocultural factors may also have influences on patient and urologist treatment choices.METHODSThe authors used bivariate and multinomial logistic regression to identify medical and sociodemographic predictors of prostatectomy (compared with radiotherapy) and curative therapy (compared with expectant management) in a cohort of 27,920 non‐Latino white, black, and Latino men without comorbidities in the latest linked Surveillance, Epidemiology and End Results–Medicare dataset (years 1995–1999). Predictors included tumor stage, patient age, marital status, race/ethnicity, and soscioeconomic status.RESULTSYounger age and higher tumor grade were robust predictors of curative treatment compared with expectant management and of prostatectomy compared with radiotherapy. Sociodemographic factors had an additive role in treatment choice. Marriage predicted curative treatment compared with expectant management (adjusted risk ratio [RR] = 1.28 [1.25–1.30]) and prostatectomy compared with radiotherapy (adjusted RR = 1.24 [1.20–1.28]). Although blacks and Latinos were just as likely as whites to receive curative treatment, blacks were significantly less likely, whereas Latinos were more likely, to receive prostatectomy compared with radiotherapy (adjusted RRs = 0.77 [0.72–83]) and 1.24 [1.18–1.30], respectively).CONCLUSIONSMarriage was positively associated with curative treatment in general, and with prostatectomy specifically. Blacks received prostatectomy less often than whites, although they did not receive less curative treatment overall. Latinos received prostatectomy more often than whites. Clinicians should recognize the importance of cultural and social forces as well as biomedical factors in decisions regarding the treatment of patients with early‐stage prostate carcinoma. Cancer 2005. © 2005 American Cancer Society.
National survival trends of young adults with sarcoma
Authors: Bleyer Archie , Montello Michael , Budd Troy , Saxman Scott 
Published: March 2005

AbstractBACKGROUNDYoung adults with cancer in the U.S. have had less improvement in survival than either younger patients or older patients. The authors attempted to determine whether similar deficits have occurred in young adults with sarcomas and, if so, then why.METHODSIn 38,144 young adults with sarcoma who were diagnosed during 1975–1998 and were followed by the National Cancer Institute's (NCI) Surveillance, Epidemiology, and End Results Program, the average annual percent change in 5‐year survival was derived as a function of patient age. National sarcoma treatment trial data were obtained on 3242 patients who were entered onto NCI‐sponsored trials during 1997–2002.RESULTS1) For patients with bone and soft‐tissue sarcomas, except Kaposi sarcoma (KS), the least survival improvement occurred between age 15 years and age 45 years. For patients with KS, the pattern was reversed, with the greatest survival increase occurring among patients ages 30–44 years. 2) The lowest participation rate in NCI‐sponsored sarcoma treatment trials occurred in patients ages 20–44 years. For patients with KS, the highest accrual rate occurred in patients ages 35–44 years. 3) The age‐dependent survival improvement and clinical‐trial accrual patterns were correlated directly (soft‐tissue sarcomas, P < 0.005; bone sarcomas, P < 0.05; KS, P = 0.06), regardless of whether the accrual profile demonstrated a decline or a peak (KS) during early adulthood.CONCLUSIONSThe lack of survival prolongation in patients age 15–44 years in the U.S. with non‐KS sarcomas may have been a result of their relative lack of participation in clinical trials. If this is true, then reversing the shortfall in survival among young adults with sarcomas, as was accomplished among patients with KS, should benefit from increased clinical trial availability, access, and participation. Cancer 2005. Published 2005 by the American Cancer Society.
Effects of 2‐(1‐hydroxypentyl)‐benzoate on platelet aggregation and thrombus formation in rats
Authors: Zhang Yi , Wang Ling , Zhang Liying , Wang Xiaoliang 
Published: March 2005

Abstract2‐(1‐hydroxypentyl)‐benzoate (dl‐PHPB), a derivate of 3‐n‐butylphthalide (NBP), is a novel therapeutic agent for treatment of cerebral ischemia. In the present study, the antiplatelet and antithrombotic activities of dl‐PHPB were evaluated in ex vivo platelet aggregation and in vivo arteriovenous (A‐V) shunt models. dl‐PHPB inhibited platelet aggregation induced by adenosine diphosphate (ADP), arachidonic acid (AA), and collagen (COL) in a dose‐dependent manner when given orally (12.9–129.5 mg/kg). The inhibitory potency was similar to 3‐n‐butylphthalide (NBP) and aspirin (ASP). Inhibition on platelet aggregation was also observed after iv administration of dl‐PHPB (1.29–12.9 mg/kg). The time‐course of these effects showed the maximal inhibition on platelet aggregation at 1 h after oral administration and 30 min after iv injection. dl‐PHPB (12.9–129.5 mg/kg, p.o.) caused dose‐dependent inhibition of thrombus formation in the rat A‐V shunt thrombosis model. These results show that dl‐PHPB is an orally and iv antiplatelet and antithrombotic agent and may be useful for treatment of ischemia stroke. Drug Dev Res 63:174–180, (2004). © 2004 Wiley‐Liss, Inc.