bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitroxyl (HNO) Release from New Functionalized N‐Hydroxyurea‐Derived Acyl Nitroso‐9,10‐dimethylanthracene Cycloadducts.
Authors: Zeng Bu‐Bing , Huang Jinming , Wright Marcus W. , King S. Bruce 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium‐Mediated Approach to Dibenzo[b,e][1,4]diazepines and Benzopyrido‐Analogues. An Efficient Synthesis of Tarpane.
Authors: Beccalli Egle M. , Broggini Gianluigi , Paladino Giuseppe , Zoni Caterina 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Phosphonates Containing Sulfur and Selenium. Synthesis of Vinylphosphonates Bearing α‐Sulfenyl, α‐Selenenyl, α‐Sulfinyl and α‐Seleninyl Moieties and Studies on Nucleophilic Addition.
Authors: Midura Wanda H. , Krysiak Jerzy A. 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 1,3,5,2λ5‐Triazaphosphinines by Intramolecular Cyclization of (N‐Cyanophosphorimidoyl)guanidines and Diguanidinophosphonium Chlorides.
Authors: Inguimbert Nicolas , Jaeger Lothar , Taillefer Marc , Biedermann Matthias , Cristau Henri‐Jean 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereospecific, Enantioselective Allylation of α‐Hydrazono Esters by Using Allyltrichlorosilanes with BINAP Dioxides as Neutral‐Coordinate Organocatalysts.
Authors: Ogawa Chikako , Sugiura Masaharu , Kobayashi Shu 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Novel 6,11‐O‐Bridged Bicyclic Ketolides via a Palladium‐Catalyzed Bis‐allylation.
Authors: Wang Guoqiang , Niu Deqiang , Qiu Yao‐Ling , Phan Ly Tam , Chen Zhigang , Polemeropoulos Alexander , Or Yat Sun 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Antioxidant Activities of a New Lignan and a Neolignan from Saururus chinensis.
Authors: Lee Woo Song , Baek Young‐Il , Kim Ju‐Ryoung , Cho Kyung‐Hyun , Sok Dai‐Eun , Jeong Tae‐Sook 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bis(trimethylsilyl)acetylene Complexes of Titanocenes and Zirconocenes: Their Recent Chemistry and Reactions with Lewis Acids
Authors: Rosenthal Uwe , Burlakov Vladimir V. , Arndt Perdita , Baumann Wolfgang , Spannenberg Anke , Shur Vladimir B. 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Design, Synthesis and Conformational Properties of Linear Analogues of Human Myelin Basic Protein Epitope MBP1‐11
Authors: Deraos S. , Tselios T. , Daliani I. , Zoumpoulakis P. , Probert L. , Troganis A. , Papathanassopoulos P. , Mavromoustakos T. , Matsoukas J. 
Published: March 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Love‐wave‐type/Rayleigh‐wave‐type SAW resonators with thick Al‐grating structure
Authors: Isobe Atsushi , Hikita Mitsutaka , Asai Kengo 
Published: March 2005

AbstractA Love‐wave SAW propagating on a thick Al grating on a LiNbO3piezoelectric substrate exhibits a resonant characteristic with an extremely small capacitance ratio γ(=3.0) while it elastically couples with Rayleigh waves in a complex manner. In the application to a resonator, the Rayleigh wave becomes spurious and must be suppressed sufficiently. With a view to development of a broadband SAW resonator for a VCO, the propagation/reflection characteristics of this Love‐wave/Rayleigh‐wave configuration are analyzed. The following results are found by simulation and experiments. (1) Both the Love wave and Rayleigh wave have extremely wide reflection bandwidths. (2) Two types of spurious Rayleigh wave appear in the resonant characteristics. (3) The stopband of the Rayleigh wave is superior in reflection characteristic to the Love wave. It is also demonstrated experimentally that a broadband SAW‐VCO can be realized if the VCO oscillation frequency is chosen within the Rayleigh wave stopband after the Al film thickness is optimized. © 2005 Wiley Periodicals, Inc. Electron Comm Jpn Pt 2, 88(4): 1–8, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjb.20157
The rat orthologue to the inhibitory receptor gp49B is expressed by neutrophils and monocytes, but not by NK cells or mast cells
Authors: Bryceson Yenan T. , Torgersen Knut Martin , Inngjerdingen Marit , Berg Siri F. , Hoelsbrekken Sigurd E. , Fossum Sigbjørn , Dissen Erik 
Published: March 2005

AbstractMouse gp49B is a member of the leukocyte immunoglobulin‐like receptor family. It is constitutively expressed by mast cells and certain myeloid cells, and expression can be induced on natural killer (NK) cells and T cells. We have cloned several rat cDNA, 78% identical to mouse gp49B at the amino acid level, that represent the rat orthologue to mouse gp49B. A mouse monoclonal antibody (WEN29) against rat gp49B was generated. By flow cytometry and Northern blot analysis, gp49B was found to be expressed by neutrophils and monocytes, but not NK cells (primary or IL‐2‐activated), T cells (resting or concanavalin A‐stimulated) or peritoneal mast cells. Following pervanadate treatment, the tyrosine phosphatase SHP‐1 was co‐immunoprecipitated with gp49B in the macrophage cell line R2. In glutathione S‐transferase pull‐down experiments, the cytoplasmic tail of rat gp49B associated with the SH2 domains of both SHP‐1 and SHP‐2, dependent on intact and phosphorylated immunoreceptor tyrosine‐based inhibition motifs (ITIM). Compared to mouse, the cytoplasmic domain of rat gp49B contains a third ITIM‐like sequence (YLYASV) that was phosphorylated by several Src family tyrosine kinases, enhanced the phosphorylation of other ITIM, and bound to the SH2 domains of SHP‐2, suggesting a role in the recruitment of downstream phosphatases.
A polyclonal anti‐vaccine CD4 T cell response detected with HLA‐DP4 multimers in a melanoma patient vaccinated with MAGE‐3.DP4‐peptide‐pulsed dendritic cells
Authors: Zhang Yi , Renkvist Nicolina , Sun Zhaojun , Schuler‐Thurner Beatrice , Glaichenhaus Nicolas , Schuler Gerold , Boon Thierry , van der Bruggen Pierre , Colau Didier 
Published: March 2005

AbstractDuring the last few years, HLA class I tetramers have been successfully used to demonstrate anti‐vaccine CD8 CTL proliferation in cancer patients vaccinated with tumor antigens. Frequencies of CTL as low as 10–6 among CD8 cells were observed even in patients showing tumor regression. Little is known about the role of tumor‐antigen‐specific CD4 T cells in the context of these anti‐vaccine responses. Therefore, we developed a very sensitive approach using fluorescent class‐II–peptide multimers to detect antigen‐specific CD4 T cells in vaccinated cancer patients. We produced HLA‐DP4 multimers loaded with the MAGE‐3243–258 peptide and used them to stain ex vivo PBL from melanoma patients injected with dendritic cells pulsed with several class I and class II tumor antigenic peptides, including the MAGE‐3243–258 peptide. The multimer+ CD4 T cells were sorted and amplified in clonal conditions; specificity was assessed by their ability to secrete IFN‐γ upon contact with the MAGE‐3 antigen. We detected frequencies of about 1×10–6 anti‐MAGE‐3.DP4 cells among CD4 cells. A detailed analysis of one patient showed an anti‐MAGE‐3.DP4 CD4 T cell amplification of at least 3000‐fold upon immunization. TCR analysis of the clones from this patient demonstrated a polyclonal response against the MAGE‐3 peptide.
Chiral capillary electrophoresis‐mass spectrometry of amino acids in foods
Authors: Simó Carolina , Rizzi Andreas , Barbas Coral , Cifuentes Alejandro 
Published: March 2005

AbstractIn this work, the development of a new chiral capillary electrophoresis‐mass spectrometry (CE‐MS) method to separate D‐ and L‐amino acids is shown. On‐line coupling between CE and MS is established through an electrospray‐coaxial sheath flow interface. Enantiomer separation is achieved by using a cheap, nonvolatile, chiral selector as β‐cyclodextrin in the background electrolyte (BGE) together with a physically coated capillary that is aimed to prevent contamination of the electrospray. The capillary coating is simple and easy to obtain as it only requires flushing of the capillary with a polymer aqueous solution for 3 min. Optimization of CE parameters (pH of BGE, type and concentration of chiral selector, and capillary inner diameter) and electrospray‐MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure) is carried out. Two different derivatization protocols of amino acids using dansyl chloride (DNS) and fluorescein isothiocyanate (FITC) are compared in terms of MS sensitivity and chiral resolution. Under optimum CE‐MS conditions it is observed that the MS sensitivity obtained for FITC‐ and DNS‐amino acids is similar (with limit of detection (LOD) in the µM range, corresponding to amounts injected in the fmol range) while chiral resolution is better for FITC‐amino acids. The optimized method is demonstrated to provide the simultaneous analysis of 15 selected amino acids (i.e., FITC‐D/L‐Asp, ‐Glu, ‐Ser, ‐Asn, ‐Ala, ‐Pro, ‐Arg, and FITC‐γ‐aminobutyric acid (GABA) in a single chiral CE‐MS run, corresponding to the main amino acids that can be found in orange. Moreover, as a result of the high resolution achieved, it is possible to detect down to 2% of D‐Asp in the presence of 98% of L‐Asp. The good possibilities of chiral CE‐MS in food analysis are corroborated through the detection of the main amino acids in a commercial orange juice (i.e., FITC‐L‐Asp, ‐Glu, ‐Ser, ‐Asn, ‐Pro, ‐Arg, and the nonchiral FITC‐GABA) as well as the determination of the fraudulent addition of synthetic amino acids (containing D‐ and L‐forms) to a fresh orange juice.
Analysis of melamine resins by capillary zone electrophoresis with electrospray ionization‐mass spectrometric detection
Authors: Cook Helmy A. , Klampfl Christian W. , Buchberger Wolfgang 
Published: March 2005

AbstractA method for the determination of the major components of (methoxymethyl)melamine resins, with quantitative analysis of unreacted melamine by capillary zone electrophoresis (CZE) using electrospray ionization‐mass spectrometry (ESI‐MS) is presented. Using a low background electrolyte (BGE) pH, components are separated according to their charge/ionic radius ratio with a distinctly different separation selectivity compared to the HPLC methods commonly employed in melamine‐resin analysis. The use of a time‐of‐flight mass spectrometer (TOF‐MS) was concluded to be necessary, as the complex samples studied required maximum sensitivity and resolution, which is clearly superior for TOF‐MS detectors over their quadrupole counterparts. A standard curve of free melamine was determined with an R2 = 0.999 over a concentration range of an order of magnitude. This method offers the unique separation selectivity of CZE as well as a quicker analysis time, especially for dimers compared to the HPLC methods used to date.
Capillary electrophoresis and matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry for analysis of the novel erythropoiesis‐stimulating protein (NESP)
Authors: Sanz‐Nebot Victoria , Benavente Fernando , Giménez Estela , Barbosa José 
Published: March 2005

AbstractNESP (novel erythropoiesis‐stimulating protein) is a recently approved hyperglycosylated analogue of human erythropoietin (EPO) with a long‐lasting effect. In this work, the capillary electrophoresis (CE) methodology proposed by the European Pharmacopoeia for the separation of EPO glycoforms has been modified for the separation of NESP glycoforms. Optimization of pH of the separation electrolyte has been fundamental in order to achieve baseline resolution of seven peaks corresponding to NESP glycoforms. Intact NESP has also been characterized by matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS). An accurate approximation to an average molecular mass of the NESP molecule has been obtained, taking into account the strong influence of laser intensity upon the MALDI‐TOF mass spectra found.
Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry
Authors: Yeh Ching‐Fen , Jiang Shiuh‐Jen 
Published: March 2005

AbstractA capillary electrophoresis‐inductively coupled plasma‐mass spectrometric (CE‐ICP‐MS) method for the speciation of six arsenic compounds, namely arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine is described. The separation has been achieved on a 70 cm length×75 µm ID fused‐silica capillary. The electrophoretic buffer used was 15 mM Tris (pH 9.0) containing 15 mM sodium dodecyl sulfate (SDS), while the applied voltage was set at +22 kV. The arsenic species in biological tissues were extracted into 80% v/v methanol‐water mixture, put in a closed centrifuge tube and kept in a water bath, using microwaves at 80°C for 3 min. The extraction efficiencies of individual arsenic species added to the sample at 0.5 µg As/g level were between 96% and 107%, except for As(III), for which it was 89% and 77% for oyster and fish samples, respectively. The detection limits of the species studied were in the range 0.3–0.5 ng As/mL. The procedure has been applied for the speciation analysis of two reference materials, namely dogfish muscle tissue (NRCC DORM‐2) and oyster tissue (NIST SRM 1566a), and two real‐world samples.
Protein profiling by capillary isoelectric focusing, reversed‐phase liquid chromatography, and mass spectrometry
Authors: Zhou Feng , Johnston Murray V. 
Published: March 2005

AbstractAn automated system for intact protein analysis is described that combines capillary isoelectric focusing (CIEF), reversed‐phase liquid chromatography (RPLC), and electrospray ionization‐mass spectrometry (ESI‐MS). Performance is demonstrated with a complex yeast enzyme concentrate. CIEF is performed with a microdialysis membrane‐based cathodic cell that permits pI fractions to be sampled and stored for subsequent LC‐MS analysis. A total of 50 µg protein is loaded onto the capillary. Ten fractions are stored which span the pI range 3–10. Each fraction is subsequently cleaned on a reversed‐phase trap column and then characterized by LC‐MS. MaxEnt1 is used to deconvolute the raw mass spectra to obtain the molecular weight (MW) of intact proteins/peptides in the sample. A two‐dimensional display of pI vs. MW is illustrated for the 500 most prevalent species as identified by MaxEnt1.
An interface Newton–Krylov solver for fluid–structure interaction
Authors: Michler C. , van Brummelen E. H. , de Borst R. 
Published: March 2005

AbstractThe numerical solution of fluid–structure interactions with the customary subiteration method incurs numerous deficiencies. We propose a novel solution method based on the conjugation of subiteration with a Newton–Krylov method, and demonstrate its superiority and beneficial characteristics. Copyright © 2004 John Wiley & Sons, Ltd.
Combinatorial gene therapy for bone regeneration: Cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7
Authors: Zhao Ming , Zhao Zhuoran , Koh Jeong‐Tae , Jin Taocong , Franceschi Renny T. 
Published: March 2005

AbstractBone morphogenetic proteins (BMPs) have demonstrated effectiveness as bone regeneration agents whether delivered as recombinant proteins or via gene therapy. Current gene therapy approaches use vectors expressing single BMPs. In contrast, multiple BMPs are coordinately expressed during bone development and fracture healing. Furthermore, BMPs likely exist in vivo as heterodimeric molecules having enhanced biological activity. In the present study, we test the hypothesis that gene therapy‐based bone regeneration can be enhanced by expressing combinations of BMPs. For in vitro studies, mesenchymal cell lines were transduced with individual adenoviruses containing BMP2, 4, or 7 cDNA under control of a CMV promoter (AdBMP2, 4, 7) or virus combinations. Significantly, combined transduction with AdBMP2 plus AdBMP7 or AdBMP4 plus AdBMP7 resulted in a synergistic stimulation of osteoblast differentiation. This synergy is best explained by formation of BMP2/7 and 4/7 heterodimers. To test in vivo biological activity, fibroblasts were transduced with specific virus combinations and implanted into C57BL6 mice. Consistent with in vitro results, strong synergy was observed using combined AdBMP2/BMP7 treatment, which induced twofold to threefold more bone than would be predicted based on the activity of individual AdBMPs. These studies show that dramatic enhancement of osteogenesis can be achieved using gene therapy to express specific combinations of interacting regenerative molecules. © 2005 Wiley‐Liss, Inc.
Transfection efficiency and uptake process of polyplexes in human lung endothelial cells: a comparative study in non‐polarized and polarized cells
Authors: Mennesson Eric , Erbacher Patrick , Piller Véronique , Kieda Claudine , Midoux Patrick , Pichon Chantal 
Published: March 2005

AbstractBackgroundFollowing systemic administration, polyplexes must cross the endothelium barrier to deliver genes to the target cells underneath. To design an efficient gene delivery system into lung epithelium, we evaluated capture and transfection efficiencies of DNA complexed with either Jet‐PEI™ (PEI‐polyplexes) or histidylated polylysine (His‐polyplexes) in human lung microvascular endothelial cells (HLMEC) and tracheal epithelial cells.MethodsAfter optimizing growth conditions to obtain a tight HLMEC monolayer, we characterized uptake of polyplexes by flow cytometry and evaluated their transfection efficiency. Polyplexes were formulated as small particles. YOYO‐labelled plasmid fluorescence intensity and luciferase activity were used as readouts for uptake and gene expression, respectively.ResultsPEI‐polyplexes were more efficiently taken up than His‐polyplexes by both non‐polarized (2‐fold) and polarized HLMEC (10‐fold). They were mainly internalized by a clathrin‐dependent pathway whatever the cell state. In non‐polarized cells, His‐polyplexes entered also mainly via a clathrin‐dependent pathway but with an involvement of cholesterol. The cell polarization decreased this way and a clathrin‐independent pathway became predominant. PEI‐polyplexes transfected more efficiently HLMEC than His‐polyplexes (107 vs. 105 relative light units (RLU)/mg of proteins) with a more pronounced difference in polarized cells. In contrast, no negative effect of the cell polarization was observed with tracheal epithelial cells in which both polyplexes had comparable efficiency.ConclusionsWe show that the efficiency of polyplex uptake by HLMEC and their internalization mechanism are polymer‐dependent. By contrast with His‐polyplexes, the HLMEC polarization has little influence on the uptake process and on the transfection efficiency of PEI‐polyplexes. Copyright © 2005 John Wiley & Sons, Ltd.
Plane waves with negative phase velocity in isotropic chiral mediums
Authors: Mackay Tom G. 
Published: March 2005

AbstractThe propagation of plane waves in an isotropic chiral medium (ICM) is investigated. Simple conditions are derived—in terms of the constitutive parameters of the ICM—for the phase velocity to be directed opposite to the direction of power flow. It is demonstrated that phase velocity and power flow may be oppositely directed, provided that the magneto‐electric coupling is sufficiently strong. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 120–121, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20742
A case study to improve the impedance bandwidth of a planar monopole
Authors: Qiu Jianming , Du Zhengwei , Lu Jianhua , Gong Ke 
Published: March 2005

AbstractThis paper presents a new technique to improve the impedance bandwidth of a rectangular planar monopole. The method involves beveling one part while keeping the other edges near the ground plane, which is connected to the antenna‐feeding port. The measured impedance bandwidth of the monopole can reach a ratio of 14:1 for a voltage standing wave ratio (VSWR) equal to 2:1. The measured radiation patterns of the antenna retain the typical monopole characteristics throughout the bandwidth. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 124–126, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20744
One‐turn stub‐loaded loop patch antenna on a small ground plane
Authors: Thaysen J. , Jakobsen K. B. 
Published: March 2005

AbstractA small 1.1‐cm3 one‐turn loop patch antenna located 2.5 mm above an 18 × 25 mm ground plane separated by a dielectric substrate with relative permittivity of 9.8 is presented. By varying the length of a thin quarter‐wavelength matching line, it is possible to change the resonant frequency. An RLC circuit model is used to determine the minimum quality factor Q. It is shown that the proposed loop yields a bandwidth performance close to the theoretical limit. The antenna has a 14.5% bandwidth at the resonant frequency of 2.04 GHz. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 126–128, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20745
Compact printed ultra‐wideband slot antenna with a band‐notched operation
Authors: Su Saou‐Wen , Wong Kin‐Lu , Chang Fa‐Shian 
Published: March 2005

AbstractA compact printed ultra‐wideband (UWB, 3.1–10.6 GHz) circular‐slot antenna with a notched frequency band at 5 GHz is presented. The antenna is a uniplanar structure and occupies a compact area of 25 × 26 mm2 only (the side length is less than 27% of the lower edge frequency at 3.1 GHz). The antenna has a U‐slotted circular stub embedded inside the circular slot. By choosing the length of the U‐slot cut in the circular stub to be about a half‐wavelength of the desired notched frequency, band‐notched UWB operation is obtained. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 128–130, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20746
A hybrid fast converging series expansion for the 2D Green's function of a multilayered medium
Authors: Rogier Hendrik 
Published: March 2005

AbstractA new formalism combining modal expansions based on perfectly matched layers (PMLs) and on Floquet modes is proposed to derive a fast‐converging series expansion for the 2D Green's function of layered media. The PML‐based modal expansion is obtained by terminating the waveguide, formed by the layered medium, with PMLs. The convergence of this series is improved by adding an expansion of Floquet modes corresponding to an auxiliary periodic problem. The period of this auxiliary problem acts as a parameter, the convergence rate of both series allowing to be controlled. The formalism is applied to a dielectric‐slab waveguide to illustrate the improved convergence and the efficiency of the new approach. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 130–134, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20747
Rectangular dielectric resonator antenna on a conductor‐backed co‐planar waveguide
Authors: Suma M. N. , Menon Sreedevi K. , Bijumon P. V. , Sebastian M. T. , Mohanan P. 
Published: March 2005

AbstractIn this paper, we present an effective excitation of a rectangular dielectric resonator antenna (DRA) with a conductor‐backed co‐planar waveguide (CB‐CPW). The radiation and resonance characteristics are found to vary, depending on the orientation of the DR on the coplanar feed line. The effect of finite and infinite ground planes of CB‐CPW on the radiation characteristics of the rectangular DRA is studied. The orientation and position of the DR are optimized for maximum gain and bandwidth. The optimized antenna geometry offers ∼10.46 dBi gain and 7.5% bandwidth with low cross‐polar radiation characteristics. © 2005 Wiley Periodicals, Inc. Microwave Opt Technol Lett 45: 154–156, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.20754