bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustained Pituitary T3 Production Explains the T4-mediated TSH Feedback Mechanism
Authors: Batistuzzo Alice , Salas-Lucia Federico , Gereben Balázs , Ribeiro Miriam O , Bianco Antonio C 
Published: October 2023

Abstract
The regulation of thyroid activity and thyroid hormone (TH) secretion is based on feedback mechanisms that involve the anterior pituitary TSH and medial basal hypothalamus TSH-releasing hormone. Plasma T3 levels can be “sensed” directly by the anterior pituitary and medial basal hypothalamus; plasma T4 levels require local conversion of T4 to T3, which is mediated by the type 2 deiodinase (D2). To study D2-mediated T4 to T3 conversion and T3 production in the anterior pituitary gland, we used mouse pituitary explants incubated with 125I-T4 for 48 hours to measure T3 production at different concentrations of free T4. The results were compared with cultures of D1- or D2-expressing cells, as well as freshly isolated mouse tissue. These studies revealed a unique regulation of the D2 pathway in the anterior pituitary gland, distinct from that observed in nonpituitary tissues. In the anterior pituitary, increasing T4 levels reduced D2 activity slightly but caused a direct increase in T3 production. However, the same changes in T4 levels decreased T3 production in human HSkM cells and murine C2C12 cells (both skeletal muscle) and mouse bone marrow tissue, which reached zero at 50 pM free T4. In contrast, the increase in T4 levels caused the pig kidney LLC-PK1 cells and kidney fragments to proportionally increase T3 production. These findings have important implications for both physiology and clinical practice because they clarify the mechanism by which fluctuations in plasma T4 levels are transduced in the anterior pituitary gland to mediate the TSH feedback mechanism.
Weight Loss Effect of GLP-1 RAs With Endoscopic Bariatric Therapy and Bariatric Surgeries
Authors: Imam Ahmad , Alim Hussam , Binhussein Mohammad , Kabli Abdulrahman , Alhasnani Husam , Allehyani Abdullah , Aljohani Ammar , Mohorjy Ahmad , Tawakul Abdullah , Samannodi Mohammed , Taha Wael 
Published: October 2023

Abstract

Background
Different treatment modalities are available for obesity management, including lifestyle changes, pharmacotherapy, endoscopic interventions, and surgeries. Limited evidence is available on the weight loss effect of combining glucagon-like peptide 1 receptor agonists (GLP-1 RAs) with endoscopic bariatric therapy (EBT) and bariatric surgeries (BS).

Objectives
In this systematic review, we compared the weight loss effect and metabolic changes of combining GLP-1 RAs with EBT and BS.

Methods
Literature searches were performed in the Cochrane Database of Systematic Review, Cochrane Central Register of Controlled Trials, Embase, PubMed, Google Scholar, and PRISMA databases. Only randomized control trials and retrospective studies were included.

Results
A total of 11 studies was included. Nine studies compared BS with and without liraglutide and 2 compared EBT with and without liraglutide. Adding liraglutide to EBT or BS provided significant weight loss when compared with EBT or BS alone. When changes in weight were compared across the studies, EBT with liraglutide showed a weight loss effect comparable to the net weight loss (ie, nadir weight loss after BS-regained weight) achieved following BS alone.

Conclusion
This review showcases a promising approach for managing obesity that combines GLP-1 RAs with EBT. This approach is expected to achieve shorter hospital stays, fewer side effects, and longer term weight loss benefits than BS alone. However, additional prospective studies with higher quality, more consistent outcome measures for weight loss and metabolic changes are needed to further evaluate the approach.
The Political Lives of Infrastructure
Authors: Attewell Wesley , Mitchell-Eaton Emily , Nisa Richard 
Published: October 2023

AbstractThis issue explores the historical production of infrastructures as places of resistance and world-building for workers, villagers, and migrants across the twentieth and early twenty-first centuries—a period when narratives about the role of infrastructure as a conduit for modernization, development, and the centralizing capacities of the state had broad purchase. Contributions invite consideration of two questions. First, what struggles do histories of infrastructural power reveal if infrastructures are delinked from master narratives tying them to state and state-backed centralization? While development, nation building, and extraction are often state-sponsored or state-backed projects, the articles here demonstrate that modern states are not the only wielders of infrastructural power. Second, how does this decentering of the state in infrastructural analyses transform the stakes of radical political activity and the work of radical historical actors? In highlighting a different, more localized scale of infrastructural production and relation building—both within and beyond the bounds of the nation-state—contributors to this issue resituate ostensibly disparate, small sites as key to larger political struggles and frame everyday forms of “getting by” as resistance.
Bound Passages
Authors: Vũ Jason Tuấn 
Published: October 2023

Abstract
This article traces the settler-colonial histories of Daniel K. Inouye International Airport (HNL) in Hawai‘i and Antonio B. Won Pat International Airport (GUM) in Guåhan (Guam) to chart the role of Indigenous dispossession in facilitating ongoing carceral transits across the Pacific. Focusing on the March 2021 deportation of thirty-three Vietnamese refugees from the United States, it situates the deportation flight’s layovers at HNL and GUM within larger processes of racial-colonial violence that constitute the development and operation of both airports. In this sense, HNL and GUM reveal the palimpsestic nature of Hawai‘i and Guåhan as sites of colonial occupation that, in turn, are used to bolster the further transit of global US empire. This essay thus attempts to chart a critical transpacific geography that links the settler-colonial and carceral dimensions of US empire through the issue of Southeast Asian deportation.
Uneven Mobilities
Authors: Valadares Desiree 
Published: October 2023

Abstract
This article studies the Hope–Princeton Highway, a regional route in the province of British Columbia (BC), Canada, through the lens of uneven mobilities. Bringing together insights from infrastructure studies, mobility studies, and settler colonial studies, uneven mobilities is a concept that historicizes mobility research in terms of colonial and carceral logics. Using this concept, the article provides insight into political actors, namely incarcerated forced laborers of Japanese descent, whose unjust confinement and forced labor on this infrastructural route remained unacknowledged until recently. The article relies on a range of archival sources that engage the visual culture of the highway and the subtle linkages between an imagined scenic landscape and an imagined multicultural Canada. The article also narrates this highway route by constructing pictorial and landscape relationships of colonialism and carcerality, linking it to uneven mobilities and economic development through the ubiquitous highway road sign—a contemporary initiative to mark and interpret sites of historical and cultural importance along this and other BC routes. The article then explores the infrastructural politics of this route at the scale of the body to highlight modes of resistance. This article advances a tentative theory of uneven mobilities by centering so-called road disturbances through acts of resistance such as rest, play, and work stoppages to reveal how uneven mobilities are entwined with the production of embodied subjectivities.
Imagining the Nation as a “Web” of Animals
Authors: Wanniarachchi Senel 
Published: October 2023

AbstractWhile existing scholarship is largely interested in exploring how a particular (nonhuman) animal symbol is mobilized to support a specific exclusionary agenda, what happens when the very nation is imagined as a “web” of different constituent “species”? In this article, the author examines four nonhuman animal symbols—the lion, the tiger, the pig, and the butterfly, which have been mobilized in Sri Lanka to delineate (imaginary) boundaries between different communities that reside there. The article combines critical animal studies and nationalism studies scholarship with affect theory to complicate the current understandings on the relationships between animality, affect, and nationalism. A focus on affect, the author argues, can open up a line of inquiry that is invisible to our current accounts on the relationships between animality and nationalism by demonstrating how animality can be instrumentalized as a tool for not only domination and subordination but also subversion, refusal, and contestation. Tracing the different ways in which animality gets mobilized to represent various communities that reside within the nation, the article highlights the complex ways in which animality can be mobilized within nation-building and how bodies negotiate and respond to such assignations.
Anteromedial Petrous (Gardner's) Triangle: Surgical Anatomy and Relevance for Endoscopic Endonasal Approach to the Petrous Apex and Petroclival Region
Authors: Coutinho da Silva Martin B. , Hernández Hernández Vanessa , Gupta Prakash , Lavinsky Joel , Zenonos Georgios A. , Wang Eric W. , Snyderman Carl H. , Gardner Paul A. 
Published: October 2023

BACKGROUND AND OBJECTIVES:
Triangular corridors have been used as reliable surgical entry points for open transcranial approaches to the petrous apex (PA) and petroclival region (PCR). The endoscopic endonasal approaches have grown rapidly in the last decade, and the indications have advanced. The knowledge of accurate and reliable anatomic landmarks through endoscopic endonasal route is essential and remain to be established. The purpose of this study was to describe the feasibility and surgical exposure of the anteromedial petrous (Gardner's) triangle as a novel corridor to the PA and PCR.

METHODS:
Five anatomic specimens were dissected. The PA and PCR were accessed through endoscopic endonasal approaches and contralateral transmaxillary approach. The limits of the anteromedial petrous (Gardner's) triangle were identified and dissected and associated measurements performed.

RESULTS:
The dissection was divided into 6 steps. The limits of the anteromedial petrous (Gardner's) triangle were identified and defined by the paraclival internal carotid artery anterolaterally, the abducens nerve posteromedially, and the petroclival synchondrosis inferiorly. Three lines were established following the limits of the triangle. The mean distance of the anterolateral limit was 10.03 mm (SD = 0.94), of the posteromedial limit was 20.06 mm (SD = 2.90), and of the inferior limit was 17.99 mm (SD = 2.99). The mean area was 87.56 mm2 (SD = 20.06). The 3 anatomic landmarks with a critical role to safely define the triangle were the pterygosphenoidal fissure, the petrosal process of the sphenoid bone, and the petroclival synchondrosis.

CONCLUSION:
The anteromedial (Gardner's) triangle is a well-defined bone corridor which provides access to the entire petrous bone and petroclival junction through endoscopic endonasal route. Regardless of the anatomic variations or tumor location, the landmarks of the abducens nerve, paraclival internal carotid artery, and petroclival synchondrosis are key for understanding lateral access to tumors extending from the clivus.
Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms
Authors: Strobl Frederic , Ratke Julia , Krämer Franziska , Utta Ana , Becker Sigrun , Stelzer Ernst H. K. 
Published: October 2023

ABSTRACT
For diploid model organisms, the actual transgenesis processes require subsequent periods of transgene management, which are challenging in emerging model organisms due to the lack of suitable methodology. We used the red flour beetle Tribolium castaneum, a stored-grain pest, to perform a comprehensive functional evaluation of our AClashOfStrings (ACOS) and the combined AGameOfClones/AClashOfStrings (AGOC/ACOS) vector concepts, which use four clearly distinguishable markers to provide full visual control over up to two independent transgenes. We achieved comprehensive statistical validation of our approach by systematically creating seventeen novel single and double homozygous sublines intended for fluorescence live imaging, including several sublines in which the microtubule cytoskeleton is labeled. During the mating procedures, we genotyped more than 20,000 individuals in less than 80 working hours, which corresponds to about 10 to 15 s per individual. We also confirm the functionality of our combined concept in two double transgene special cases, i.e. integration of both transgenes in close proximity on the same chromosome and integration of one transgene on the X allosome. Finally, we discuss our vector concepts regarding performance, genotyping accuracy, throughput, resource saving potential, fluorescent protein choice, modularity, adaptation to other diploid model organisms and expansion capability.
Spatial transcriptomics map of the embryonic mouse brain – a tool to explore neurogenesis
Authors: Di Marco Barbara , Vázquez-Marín Javier , Monyer Hannah , Centanin Lázaro , Alfonso Julieta 
Published: October 2023

ABSTRACT
The developing brain has a well-organized anatomical structure comprising different types of neural and non-neural cells. Stem cells, progenitors and newborn neurons tightly interact with their neighbouring cells and tissue microenvironment, and this intricate interplay ultimately shapes the output of neurogenesis. Given the relevance of spatial cues during brain development, we acknowledge the necessity for a spatial transcriptomics map accessible to the neurodevelopmental community. To fulfil this need, we generated spatially resolved RNA sequencing (RNAseq) data from embryonic day 13.5 mouse brain sections immunostained for mitotic active neural and vascular cells. Unsupervised clustering defined specific cell type populations of diverse lineages and differentiation states. Differential expression analysis revealed unique transcriptional signatures across specific brain areas, uncovering novel features inherent to particular anatomical domains. Finally, we integrated existing single-cell RNAseq datasets into our spatial transcriptomics map, adding tissue context to single-cell RNAseq data. In summary, we provide a valuable tool that enables the exploration and discovery of unforeseen molecular players involved in neurogenesis, particularly in the crosstalk between different cell types.
Keep Off My Turf! Low-Status Managers’ Territoriality as a Response to Employees’ Novel Ideas
Authors: Venkataramani Vijaya , Derfler-Rozin Rellie , Liu Xin , Mao Jih-Yu 
Published: October 2023

Although employees come up with creative (i.e., novel and useful) ideas, many of those ideas are not endorsed or implemented by managers. In shedding light on this phenomenon, we propose that managers who have lower social status in the organization are more likely to reject employees’ novel (but still useful) ideas. Guided by associative-propositional evaluation theory (AP-E) and the literature on the psychology of having low status, we hypothesize that when employees propose novel (compared with more mundane) ideas, it triggers greater feelings of insecurity and threat in low-status (versus high-status) managers, who perceive that these employees, if successful, could potentially infringe on their own domains at work. In turn, such low-status managers feel the need to be territorial—that is, to maintain and protect their existing work domains from potential infringement by others—and therefore refrain from endorsing their employees’ novel, yet useful ideas. However, we suggest that such negative effects are attenuated when low-status managers have high levels of organizational identification, allowing them to subordinate their self-interest to the interests of the broader organization. We demonstrate these effects in four preregistered studies—three laboratory experiments and a field study (with real employee ideas provided to managers for their assessment). We discuss the implications for the literature on the receiving side of creativity, territoriality, and status in organizations. Funding: This research was partly supported by the National Natural Science Foundation of China [Grant 72002214, 72372151 and 72302122] and Humanities and Social Sciences Research of Ministry of Education of China [Grant 22YJC630105]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/orsc.2021.15132 .
Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis
Authors: Hadipour Mahboubeh , Darani Hossein Yousofi , Talebzadeh Hamid , Eslamian Mohammad , Aboutalebian Shima , Harandi Majid Fasihi , Mirhendi Hossein 
Published: October 2023

Background
Developing more sensitive methods for the diagnosis of echinococcosis is essential. In this study PCR assay for sensitive detection of specific cell-free DNA (cfDNA) of Echinococcus granulosus sensu lato in the sera of the sheep naturally infected with echinococcosis was investigated.

Methods
To extract cfDNA from 35 infected sheep, the modified phenol-chloroform method was used for two different volumes (0.5 and 2 ml) of serum samples. From each extracted sample, two DNA volumes (5 and 10 μl) were amplified using both standard PCR and semi-nested PCR targeting NADH dehydrogenase subunit I.

Results
Standard and semi-nested PCR on 0.5 ml of serum samples detected Echinococcus DNA in 8 and 12 out of 35 sheep, respectively; however, using 2 ml of serum samples, they detected 24 and 27 samples. By increasing the volume of template DNA, the PCRs could detect 29 and 33 out of 35 samples. The results were confirmed by sequencing of randomly selected PCR amplicons and comparing them with GenBank databases.

Conclusions
Larger volumes of serum for DNA extraction, greater volumes of DNA template for PCR, and employing a semi-nested PCR protocol, increased the sensitivity of PCR to 95%. This approach can also be applied to the diagnosis of echinococcosis in humans.
Electrocardiographic interpretation by emergency medical services professionals in Saudi Arabia: A cross sectional study
Authors: Alalwan Mohammed Abdullah , Alshammari Talal , Alawjan Hassan , Alkhayat Hassan , Alsaleh Ahmed , Alamri Ibrahim , Aldubaikel Alaa , Alqahtani Jaber , Alrawashdeh Ahmad , Alqahtani Saeed 
Published: October 2023

BackgroundManagement of acute myocardial infarction (AMI) and cardiac arrhythmias in prehospital settings is largely determined by providers of emergency medical services (EMS) who can proficiently interpret the electrocardiography (ECG). The aim of this study was to assess the ECG competency of EMS providers in Saudi Arabia.MethodsBetween Aug and Sep 2022, we invited all EMS providers working for the Saudi Red Crescent Authority in Makkah, Riyadh, and Sharqiyah regions to complete a cross-sectional survey. The survey was used to assess the ability of EMS providers to interpret 12 ECG strips. Characteristics and ECG competency were summarized using descriptive statistics. Differences in ECG competency across paramedics with lower and higher qualifications were assessed.ResultsDuring the study period, 231 participants completed the survey, and all were included. The overall mean age was 33.4, and most participants were male (94.8%). Nearly half of the participants were paramedics with an associate degree and 46.4% were paramedics with higher degrees. The average rate of correct answers to the 12 ECG strips was 43.3% (95% CI: 35.4%, 51.3%). Atrial flutter, ventricular fibrillation, atrial fibrillation, 3rddegree heart block, and ventricular tachycardia were identified by 52.8%, 60.2%, 42.0%, 40.7%, and 49.4% of the participants, respectively. The strip with an AMI was identified by 41.1%, while a pathological Q wave and ventricular extrasystole were identified by 19.1% and 24.7%, respectively. Paramedics with higher qualifications were as 28.0%-61.0% more likely to correctly interpret the 12 ECG strips compared to those with an associate degree (p-value across all variables was ≤ 0.001).ConclusionWhile the majority of participants in our region were unable to correctly answer the 12 ECG questionnaire, paramedics with higher qualifications were. Our study indicates that there is a need for evidenced-based ECG curricula targeting different levels of EMS professionals.
Ethnopharmacology, Antioxidant Activities, and Phytochemical Screening of Bioactive Extracts from the Seeds of Alangium Salvifolium, A Medicinal Plant
Authors: Tiwari Renu , Sharma Tripti 
Published: October 2023

Phytochemical with antioxidant properties have great potential to combat plant and human diseases. This study aims to analyze the effects of different phytochemical constituents on different solvents on their extraction yield and also to determine the bioactive phytochemical and the amount of antioxidant activity of Alangium salvifolium seeds. For this study, the seeds of Alangium salvifolium were extracted, analyzed for phytochemical constituents, and evaluated for their antioxidant activity. Chemical analysis of phytochemical constituents was performed according to standard protocols. The antioxidant capacity of the plant extracts was measured using several in vitro techniques, including the DPPH radical scavenging assay and SOS activity. The plant extracts were subjected to phytochemical screening to determine their potential for antioxidant activity and the presence of bioactive components. The results of the current study show the ethanolic extract of Alangium salvifolium seeds showed remarkable antioxidant properties. Alangium salvifolium is a remarkable medicinal plant that contains a variety of bioactive secondary plant compounds and can be used to prevent several diseases. Recent research suggests that plant-derived antioxidants that scavenge free radicals may be useful in treating chronic diseases caused by free radicals.
Effect of Cladding Material and Date of Transplanting on Growth and Yield of Greenhouse Broccoli During Winter in High Altitude Ladakh, India
Authors: Dolma Tsering , Katiyar Anand K , Kumar Rohit , Chaurasia O P , Stobdan Tsering 
Published: October 2023

Broccoli is a cool-season crop. The optimum temperature for initiation of flowering and head growth is 15-20°C. Due to subfreezing temperatures at night, the crop is not traditionally cultivated in winter in the high-altitude Ladakh region. We found that growing broccoli in a passive solar greenhouse in winter is feasible. The choice of greenhouse cladding material and the date of transplanting are important factors that need to be considered. The mean marketable head weight of the cv. Fiesta and KTS-1 in a passive solar trench greenhouse with polycarbonate cladding material transplanted on 8 September were 403±106 and 169±100 g, respectively. Delayed transplanting on 8 October did not produce any marketable head. Covering the greenhouse with a polyethylene sheet did not result in marketable heads on both transplanting dates. We anticipate our study to be a starting point for researchers and the farming community to optimize the greenhouse production of broccoli during freezing winter-months in high-altitude regions.