bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copper‐Free Click Chemistry‐Mediated Assembly of Single Quantum Dot Nanosensor for Accurately Monitoring Locus‐Specific m6A in Cancer Cells†
Authors: Li Yue‐Ying , Zhao Ning‐Ning , Geng Yi‐Xuan , Han Qian , Qiu Jian‐Ge , Jiang Bing‐Hua , Wang Zi‐Yue , Zhang Chun‐Yang 
Published: October 2023

Comprehensive SummaryN6‐methyladenosine (m6A) plays an important role in embryogenesis, nuclear export, transcription splicing, and protein translation control. Herein, we demonstrate a copper‐free click chemistry‐mediated assembly of single quantum dot (QD) nanosensor for accurately monitoring locus‐specific m6A in cancer cells. The m6A‐sensitive endoribonuclease MazF can digest the unmethylated A‐RNA, and the intact m6A‐RNA then hybridizes with DNA probes a and b to produce a sandwich hybrid, initiating the click chemistry to generate probe a–b ligation product via first tandem ligation detection reaction (LDR) cycle. Subsequently, DNA probes c and d can hybridize with the probe a–b ligation product to generate the probe c–d ligation product via second LDR cycle. Both LDR cycles can be repeated through denaturation and annealing reaction to generate abundant biotin‐/fluorophore‐modified probe c–d ligation products that can easily assemble on the QD surface to induce distinct fluorescence resonance energy transfer (FRET) between QD and Cy5. This assay can be homogenously performed without the involvement of copper catalyst, m6A‐specific antibody, radioactive labeling, ligase enzyme, enzymatic reverse transcription, and next‐generation sequencing. Moreover, it can discriminate even 0.01% m6A level in complex samples and accurately measure cellular m6A‐RNA expression, providing a promising avenue for clinical diagnostics and biomedical research.
Fused mean structure learning in data integration with dependence
Authors: Hector Emily C. 
Published: October 2023

AbstractMotivated by image‐on‐scalar regression with data aggregated across multiple sites, we consider a setting in which multiple independent studies each collect multiple dependent vector outcomes, with potential mean model parameter homogeneity between studies and outcome vectors. To determine the validity of a joint analysis of these data sources, we must learn which of them share mean model parameters. We propose a new model fusion approach that delivers improved flexibility and statistical performance over existing methods. Our proposed approach specifies a quadratic inference function within each data source and fuses mean model parameter vectors in their entirety based on a new formulation of a pairwise fusion penalty. We establish theoretical properties of our estimator and propose an asymptotically equivalent weighted oracle meta‐estimator that is more computationally efficient. Simulations and an application to the ABIDE neuroimaging consortium highlight the flexibility of the proposed approach. An R package is provided for ease of implementation.
Cuproptosis‐related molecular patterns and gene (ATP7A) in hepatocellular carcinoma and their relationships with tumor immune microenvironment and clinical features
Authors: Li Shanbao , Weng Junyong , Xiao Chao , Lu Jing , Cao Wanyue , Song Fangbin , He Zeping , Zhang Peng , Zhu Zhonglin , Xu Junming 
Published: October 2023

AbstractBackgroundCuproptosis has been studied in various aspects as a new form of cell death.AimsWe hope to explore the molecular patterns and genes related to cuproptosis in evaluating and predicting the prognosis of hepatocellular carcinoma (HCC), as well as the impact of tumor immune microenvironment.Methods and ResultsSixteen cuproptosis related gene (CRGs) and cuproptosis related molecular and gene characteristics were comprehensively analyzed from 492 HCC samples. Cuproptosis related molecular patterns were generated by consensus clustering algorithm, including cuproptosis clusters, cuproptosis gene clusters (CGC) and cuproptosis score (CS). The characteristics of tumor microenvironment (TME) and tumor immune cells were described by the ssGSEA and ESTIMATE algorithms. Cuproptosis score was established to assess the clinical characteristics, prognostic and immunotherapy. The role and mechanism of CRG (ATP7A) in HCC, as well as its relationship with TME and immune checkpoints, have been further explored. The results of somatic mutation, copy number variations (CNV), and CRGs expression in HCC suggested the CRGs might participate in the HCC oncogenesis. The cuproptosis clusters were closely related to the clinical pathological characteristics, biological processes, and prognosis of HCC. The three CGC was revealed to be consistent with the three immune infiltration characterizations, including immune‐high, immune‐mid, and immune‐low subtypes. Higher CS was characterized by decreased TMB, activated immunity, higher immune cell proportion score (IPS) and better overall survival (OS), which indicated higher CS was immune‐high type and with better treatment effect and prognosis. The ATP7A had the highest hazard ratio (HR = 1.465, p < .001), was high expression in HCC tissues and with a shorter 5‐year OS. Knocking down ATP7A could enhance intracellular copper concentration, cause a decrease in DLAT expression, and induce cuproptosis and inhibit cell proliferation and migration. ATP7A was also positively correlated with most cancer immune cells and immune checkpoints.ConclusionTaken together, this research revealed the cuproptosis related molecular patterns and genes associated with the clinical pathological characteristics, TME phenotype and prognosis of HCC. The CS will further deepen our understanding of the TME characteristics of HCC, and the involvement of ATP7A in cuproptosis will provide new ideas for predicting HCC prognosis and immunotherapy.
Strategies adopted by oral physicians, oral and maxillofacial surgeons, and oral pathologists in patient education on oral cancer: A Nigerian study
Authors: Kanmodi Kehinde K. , Salami Afeez A. , Gbadamosi Adam A. , Nwafor Jacob N. , Amoo Babatunde A. , Adisa Akinyele O. , Aladelusi Timothy O. , Almu Bello , Amzat Jimoh , Braimah Ramat O. , Ogbeide Mike E. , Okwuosa Chukwubuzor U. 
Published: October 2023

AbstractBackgroundThe burden of oral cancer in Nigeria is increasing. Different studies have shown how public education on oral cancer have increased knowledge of oral cancer across populations, however, it is not known if these practices are adopted by oral physicians, oral and maxillofacial surgeons, and oral pathologists in Nigeria.AimsTo investigate the patient oral cancer education strategies adopted by oral physicians, oral and maxillofacial surgeons, and oral pathologists in Nigeria.MethodsThis study adopted an analytical cross‐sectional study design. This study surveyed practicing oral physicians, oral and maxillofacial surgeons, and oral pathologists in Nigeria. An e‐questionnaire was used for this study. The data were analyzed using the SPSS Version 20 software, and ap‐value of <.05 was used to determine the level of statistical significance.ResultsThe study's response rate was 46.6% (75/161). The 75 participants were from the six geopolitical zones in Nigeria responded to the survey questionnaire. Even though more than half (43/75, 57.3%) of the respondents have never received any training since their post‐bachelor's degree qualification on the strategies that can be used in educating patients on oral cancer, majority (54/75, 72.0%) of them knew at least one education strategy; also, the most known (36/54, 66.7%) and utilized (33/54, 61.3%) strategy among those respondents who were aware of patient education strategy was health talk. Only 38.7% (29/75) of the respondents reported that health learning materials (posters, leaflets, fliers, and flipcharts) are available in their clinics, all of which were in insufficient quantities. Also, 93.3% (70/75) of the respondents opined that it is worthwhile that dental clinics/hospitals in Nigeria invest in the provision of oral cancer learning materials for patient use. Inferential statistical analysis did not reveal any significant relationship between the respondents' characteristics and their awareness and practice on patient oral cancer education strategies.ConclusionThis study identified that many oral physicians, oral and maxillofacial surgeons, and oral pathologists in Nigeria lack the needed capacity to educate their patients on oral cancer. There is a need to strengthen their capacity by giving them training on patient oral cancer education strategies, and by providing them with good quality and enough teaching aids.
Genetic Manipulation of Klebsiella pneumoniae
Authors: Ring Brooke E. , Khadka Saroj , Pariseau Drew A. , Mike Laura A. 
Published: October 2023

AbstractKlebsiella pneumoniae is a Gram‐negative, rod‐shaped bacterium commonly found in the human intestine. Although it typically exists as part of the normal flora, it can also cause healthcare‐associated infections with severe consequences. Understanding the specific genes responsible for its virulence through genetic manipulation is crucial for potential therapeutic interventions. However, manipulating K. pneumoniae presents challenges due to its exopolysaccharide capsule. This article presents a comprehensive collection of protocols designed to facilitate the genetic manipulation of K. pneumoniae. By following these protocols, researchers will acquire the necessary skills to prepare electrocompetent cells, utilize electroporation for efficient plasmid DNA introduction, construct isogenic mutants using the λ Red recombinase system, and generate a complementation vector for restoring the phenotypic traits of knockout strains. These protocols provide valuable tools and techniques to navigate the intricacies associated with studying and modifying K. pneumoniae. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Preparing electrocompetent K. pneumoniae cellsAlternate Protocol 1: Preparing electrocompetent K. pneumoniae cells for recombineeringBasic Protocol 2: Transforming K. pneumoniae using electroporationBasic Protocol 3: Constructing isogenic mutants in K. pneumoniae using the λ Red recombinase systemSupport Protocol 1: Confirming a knockout via colony PCRSupport Protocol 2: Verifying absence of secondary mutationsBasic Protocol 4: Generating unmarked knockout mutants in K. pneumoniae using the pFLP plasmidBasic Protocol 5: Constructing a complementation vector for K. pneumoniae
A Monoanionic Pincer Ligand with Pendant Phosphazene Arms: Stabilization of Main‐Group and Transition Metal Complexes
Authors: Li Kunlong , Huang Minghao , Zhou Jiliang 
Published: October 2023

AbstractAn unprecedented anionic pincer ligand with pendant phosphazene arms [2,6‐(MesN=PPh2)2C5H4N]− (LH) has been synthesized through a facile reaction of a bis(iminophosphoranyl)‐pyridine ligand with K‐selectride. LH can stabilize main‐group and transition metal complexes LHGeCl, LHSnCl, LHZnCl, and LHCdI. X‐ray diffraction analysis and theoretical study show that the phosphazene arms of LH strongly coordinate to the metal centers, while the central amide group alternatively donates one or two pairs of electrons to bind with different metal centers.
Thiuram Disulfides for the Fast and Efficient Synthesis of Peptide Disulfide Bonds Under Aqueous Conditions
Authors: Song Changying , Ma Dongying , Wang Yafang , Shen Shigang , Huo Shuying 
Published: October 2023

AbstractDisulfide bonds play a critical role in the pharmaceutical and biological properties of peptides. In this work, disulfiram and thiram were investigated to synthesize disulfide bonds in peptides. Excellent purities of single disulfide bond‐containing peptides were achieved with a fast reaction rate. Furthermore, one‐pot regioselective synthesis of two disulfide bonds was successfully achieved by the combination of disulfiram or thiram with trans‐[PtBr2(CN)4]2− under aqueous conditions.
Extrapolation of Metal Toxicity Data for the Rotifer Brachionus calyciflorus Using an Individual-Based Population Model
Authors: Viaene Karel P. J. , De Schamphelaere Karel A. C. , Van Sprang Patrick 
Published: October 2023

Abstract
Ecological risk assessment (ERA) of metals typically starts from standardized toxicity tests, the data from which are then extrapolated to derive safe concentrations for the envisioned protection goals. Because such extrapolation in conventional ERA lacks ecological realism, ecological modeling is considered as a promising new approach for extrapolation. Many published population models are complex, that is, they include many processes and parameters, and thus require an extensive dataset to calibrate. In the present study, we investigated how individual-based models based on a reduced version of the Dynamic Energy Budget theory (DEBkiss IBM) could be applied for metal effects on the rotifer Brachionus calyciflorus. Data on survival over time and reproduction at different temperatures and food conditions were used to calibrate and evaluate the model for copper effects. While population growth and decline were well predicted, the underprediction of population density and the mismatch in the onset of copper effects were attributed to the simplicity of the approach. The DEBkiss IBM was applied to toxicity datasets for copper, nickel, and zinc. Predicted effect concentrations for these metals based on the maximum population growth rate were between 0.7 and 3 times higher in all but one case (10 times higher) than effect concentrations based on the toxicity data. The size of the difference depended on certain characteristics of the toxicity data: both the steepness of the concentration–effect curve and the relative sensitivity of lethal and sublethal effects played a role. Overall, the present study is an example of how a population model with reduced complexity can be useful for metal ERA. Environ Toxicol Chem 2024;43:324–337. © 2023 SETAC
Alternative locust control with a dilutable linseed oil emulsion
Authors: Hartbauer Manfred , Kostarakos Konstantinos 
Published: October 2023

AbstractLocust swarm control is still a main topic in agriculture and is often based on the extensive use of chemical agents that can be problematic for the environment and human health. Currently, only a few alternative control agents are available on the market, such as neem oil, the fungus Metarhizium sp., and the microsporidian Paranosema locustae. In this study, we improved the formulation of a botanical insecticide that is based on linseed oil and was found to be effective against the desert locust, Schistocerca gregaria. To make sure that linseed oil is most effective, we first tested several natural plant oils in a comparative laboratory study. Linseed oil performed best and resulted in 100% mortality within only 2 days after a single spray treatment. However, this linseed oil formulation contains three expensive essential oils, thus we further improved it to avoid costly essential oils. A much lower concentration of linseed oil was also found to be highly effective and killed adult S. gregaria within 3 days in an experiment performed under semifield conditions. Furthermore, a concentrated stock solution of this botanical insecticide was developed to increase stability and reduce shipping costs in areas where water for dilution is available. Mechanical treatment, applied during the preparation of this linseed oil emulsion, resulted in the formation of micro‐colloids, which prevent the separation of phases and enable 1:16 dilution with water containing bicarbonate. Interestingly, only 24 h after single spray treatment, locusts became immobile and most of them were unable to jump, which would make them easy prey for natural enemies. Later, insects died due to the hardening of the linseed oil emulsion. This novel linseed oil emulsion is harmless for humans, rather easy to produce, and remains stable over weeks in its concentrated form, which suggests that it is promising alternative agent for locust control.
LncRNA HOTAIR regulates the PI3K/AKT pathway via the miR‐126‐3p/PIK3R2 axis to participate in synovial angiogenesis in rheumatoid arthritis
Authors: Liu Feifei , Wang Yuan , Huang Dan , Sun Yanqiu 
Published: October 2023

AbstractBackgroundThe abnormal expression of long noncoding RNA (LncRNA) HOTAIR has been associated with synovial angiogenesis in rheumatoid arthritis (RA). The aim of this study is to investigate whether LncRNA HOTAIR plays a role in synovial angiogenesis in RA by regulating the phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) pathway through the miR‐126‐3p/PIK3R2 axis.MethodsIn this study, we conducted in vitro experiments by designing overexpression plasmids and small interfering RNAs targeting LncRNA HOTAIR and then transfected them into rheumatoid arthritis fibroblast‐like synoviocytes (RA‐FLS). We then co‐cultured the RA‐FLS with human umbilical vein endothelial cells (HUVEC) to establish a RA‐FLS‐induced HUVEC model. We investigated the effects of LncRNA HOTAIR on the proliferation, migration, lumen forming ability of HUVEC, as well as the expression of synovial endothelial cell markers, angiogenic factors, and the PI3K/AKT pathway. To validate the interactions between LncRNA HOTAIR, miR‐126‐3p, and PIK3R2, we used bioinformatics and luciferase reporter experiments. We also employed real‐time fluorescence quantitative, Western blotanalysis, and immunofluorescence techniques to analyze the target genes and proteins.ResultsThe expression of LncRNA HOTAIR was upregulated in HUVEC induced by RA‐FLS. The overexpression of LncRNA HOTAIR significantly increased the expression of vascular endothelial growth factor, basic fibroblast growth factor, CD34, and CD105 in HUVEC, promoting their proliferation, migration, and lumen formation. At the same time, the overexpression of LncRNA HOTAIR inhibited the expression of miR‐126‐3p, promoted the expression of PIK3R2, activated the PI3K/AKT pathway, and promoted the expression of PI3K, AKT and phosphorylated‐AKT, while the silence of LncRNA HOTAIR reversed these expressions. Bioinformatics and double luciferase reporter gene experiments confirmed the targeting relationship among LncRNA HOTAIR, miR‐126‐3p, and PIK3R2. Finally, the rescue experiments showed that PI3K agonists could reverse the inhibitory effect of silent LncRNA HOTAIR on HUVEC.ConclusionLncRNA HOTAIR has the potential to activate the PI3K/AKT pathway, likely through the regulatory axis involving miR‐126‐3p/PIK3R2, consequently contributing to synovial angiogenesis in RA.
Evaluation of an Open‐Loop, Computer‐Based Infusion System Designed to Achieve a Series of Constant, Targeted Plasma Procainamide Concentrations in Patients Undergoing Electrophysiologic Testing
Authors: Coyle James D. , Carnes Cynthia A. , Schaal Stephen F. 
Published: October 2023

Study Objective. To evaluate the performance of a computer‐based procainamide infusion system in patients undergoing electrophysiologic testing.Design. Prospective case series.Setting. Electrophysiology laboratory in a university hospital.Patients. Thirty‐four patients with inducible sustained ventricular tachycardia.Interventions. Intravenous infusion of procainamide to achieve and maintain targeted plasma concentrations.Measurements and Main Results. System performance was assessed by comparing targeted and observed plasma concentrations. The population median absolute performance error (size of typical miss) was 12.6% (95% CI 11.2–14.1%). The population median performance error (system bias) was not significantly different from zero. A small but statistically significant improvement in performance over time was observed (population absolute performance error divergence −0.125%/min). Population wobble (overall system stability) was 7.6% (95% CI 6.8–8.3%). Population‐based estimates of central compartment volume and volume of distribution at steady state were significantly higher and lower, respectively, than estimates used by the infusion system.Conclusion. The computer‐based infusion system is capable of achieving and maintaining a series of targeted procainamide concentrations in patients undergoing electrophysiologic testing.
Time‐Dependent Disposition of Tacrolimus and Its Effect on Endothelin‐1 in Liver Allograft Recipients
Authors: Min David I. , Chen Hsiang‐Yin , Lee Myung‐Koo , Ashton Kay , Martin Maureen F. 
Published: October 2023

Study Objective. To characterize a time‐dependent disposition of oral tacrolimus and its relationship with plasma endothelin‐1 concentrations.Design. Randomized, crossover study.Setting. Clinical research center of a university‐affiliated hospital.Patients. Twelve stable liver transplant recipients.Interventions. In the steady state, 23 blood samples were taken from each patient before and after tacrolimus administration over 24 hours.Measurements and Main Results. Whole blood samples for tacrolimus and plasma endothelin‐1 were analyzed by enzyme immunoassay. The relationship of their concentrations and their pharmacokinetic parameters between morning and evening doses were compared. The area under the curve (AUC) of tacrolimus in the morning dose was significantly larger than that in the evening dose (219 ± 54 ng•hr/ml and 188 ± 57 ng•hr/ml, respectively, p=0.004). The mean time to peak concentration (Tmax) was significantly shorter for the morning dose than for the evening dose (1.6 ± 0.7 hrs and 3.5 ± 2.9 hrs, respectively, p=0.01). The mean peak concentration (Cmax) was significantly higher in the morning dose than in the evening dose (32.2 ±9.1 ng/ml and 21.6 ± 8.3 ng/ml, respectively, p=0.008). However, the mean trough concentration (Cmin) was not significantly different between doses. Endothelin‐1 concentrations followed the same pattern as tacrolimus, with AUC and Cmax for the morning significantly higher than those for the evening dose of tacrolimus (AUC 13.8 ± 3.7 pg•hr/ml, morning, and 11.0 ± 3.5 pg•hr/ml, evening, p=0.005; Cmax 2.4 ± 1.1 pg/ml morning, and 1.5 ± 0.6 pg/ml evening, p=0.02). Tacrolimus levels did not correlate with endothelin‐1 levels (r2=0.06, p=0.001).Conclusions. Tacrolimus disposition in liver transplant patients is determined by time of administration. Plasma endothelin‐1 concentrations follow the same pattern as blood tacrolimus concentrations.
Acetaminophen‐Related Acute Renal Failure without Fulminant Liver Failure
Authors: Eguia Luis , Materson Barry J. 
Published: October 2023

Background. Our patient experienced acute renal failure but not fulminant hepatic failure from acetaminophen toxicity.Objective. To review the clinical and laboratory characteristics of similar cases of acetaminophen nephrotoxicity.Methods. A MEDLINE search and medical record search at two large teaching hospitals.Results. We reviewed our index case, a patient at Jackson Memorial Hospital, and 34 additional patients with acetaminophen nephrotoxicity reported in the literature. Oliguria was present in 23 of 31 patients. There was no difference in peak serum creatinine levels between patients treated with N‐acetylcysteine and those not treated. The onset of acute renal failure was from 2–5 days after overdose, and peak serum creatinine levels occurred 3–16 days (average 7.3 days) after overdose. Thirteen patients required hemodialysis; all but one were oliguric. Renal failure was spontaneously reversible in all patients.Conclusion. Although uncommon, it is possible to have acute renal failure due to acetaminophen toxicity in the absence of fulminant hepatic failure.