bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glymphatic system clearance and Alzheimer’s disease risk: a CSF proteome-wide study
Authors: Cullell Natalia , Caruana Giovanni , Elias-Mas Andrea , Delgado-Sanchez Ariane , Artero Cristina , Buongiorno Maria Teresa , Almería Marta , Ray Nicola J. , Correa Sonia A. L. , Krupinski Jerzy 
Published: January 2025

Abstract

Background
The emerging evidence of the role of the glymphatic system (GS) in Alzheimer’s disease (AD) provides new opportunities for intervention from the earliest stages of the disease. The aim of the study is to evaluate the efficacy of GS in AD to identify new disease biomarkers.

Methods
We performed a two-stage proteomic study to evaluate the GS health using intravenous gadolinium-based contrast agent (GBCA) with serial T1 3T magnetic resonance imaging (MRI) in individuals with amnestic mild cognitive impairment (aMCI). In Stage 1 (evaluated in the Cohort 1 of aMCI participants (n = 11)), we correlated the levels of 7K cerebrospinal fluid (CSF) proteins (estimated by SOMAscan) with GS health in 78 Freesurfer-segmented brain regions of interest (ROIs).

Results
A total of seven different proteins were significantly associated with GS health (p-value < 6.4 × 10–4). The stronger correlations were identified for NSUN6, GRAAK, OLFML3, ACTN2, RUXF, SHPS1 and TIM-4. A pathway enrichment analysis revealed that the proteins associated with GS health were mainly implicated in neurodegenerative processes, immunity and inflammation. In Stage 2, we validated these proteomic results in a new cohort of aMCI participants (with and without evidence of AD pathology in CSF (aMCI(-) and aMCI/AD( +); n = 22 and 7, respectively) and healthy controls (n = 10). Proteomic prediction models were generated in each ROI. These were compared with demographic-only models for identifying participants with aMCI(-) and aMCI/AD( +) vs controls. This analysis was repeated to determine if the models could identify those with aMCI/AD( +) from both aMCI(-) and controls. The proteomic models were found to outperform the demographic-only models.

Conclusions
Our study identifies proteins linked with GS health and involved the immune system in aMCI participants.
Applications of liposomes and lipid nanoparticles in cancer therapy: current advances and prospects
Authors: Cheng Zhe , Huang Huichao , Yin Meilong , Liu Huaizheng 
Published: January 2025

Abstract
Liposomes and lipid nanoparticles are common lipid-based drug delivery systems and play important roles in cancer treatment and vaccine manufacture. Although significant progress has been made with these lipid-based nanocarriers in recent years, efficient clinical translation of active targeted liposomal nanocarriers remains extremely challenging. In this review, we focus on targeted liposomes, stimuli-responsive strategy and combined therapy in cancer treatment. We also summarize advances of liposome and lipid nanoparticle applications in nucleic acid delivery and tumor vaccination. In addition, we discuss limitations and challenges in the clinical translation of these lipid nanomaterials and make recommendations for the future research in cancer therapy.

Graphical Abstract
Ningxiang pig-derived lactobacillus reuteri modulates host intramuscular fat deposition via branched-chain amino acid metabolism
Authors: Yang Mei , Xie Qian , Wang Jing , Zha Andong , Chen Jiashun , Jiang Qian , Kang Meng , Deng Qiuchun , Yin Yulong , Tan Bie 
Published: January 2025

Abstract

Background
Gut microbiota has been extensively demonstrated to modulate host lipid metabolism. Higher intramuscular fat (IMF) accumulation in Chinese indigenous breed pigs is associated with their special gut microbiota structure. However, the specific microbes and metabolic pathways responsible for lipid deposition are still poorly understood.

Results
In the present study, a comparative analysis of the gut microbiota and metabolome in obese Ningxiang (NX) pigs and lean Duroc × Landrace × Yorkshire (DLY) pigs was conducted. The results revealed a higher abundance of gut lactobacilli and a correlation of branched-chain amino acid (BCAA) metabolism pathway in NX pigs. We proceeded to verify the roles of various lactobacilli strains originating from NX pigs in BCAA metabolism and lipids deposition in SD rats. We demonstrated that L. reuteri is a fundamental species responsible for modulating lipid deposition in NX pigs and that increased circulating levels of BCAA are positively linked to greater lipid deposition. Additionally, it has been verified that L. reuteri originating from NX pigs has the ability to improve BCAA synthesis in the gut and enhance IMF content in lean DLY pigs. The expression of genes related to lipid synthesis was also significantly upregulated.

Conclusions
Taken together, our results imply that NX pig-derived L. reuteri regulates BCAA metabolism and plays a potential role in improving the meat quality of lean pig breeds through modulation of host intramuscular lipid deposition. The results provide a new strategy for improving the meat quality of commercial pigs by influencing host metabolism through supplementing dietary additives.
The role of optical coherence tomography angiography in assessing diabetic choroidopathy: a systematic review
Authors: Nowroozzadeh M. Hossein , Bagheri Mansoureh 
Published: January 2025

Abstract

Background
Diabetic retinopathy (DR) is a leading cause of vision impairment worldwide, affecting both retinal and choroidal vasculature. While advances in imaging technology, particularly optical coherence tomography angiography (OCTA), provide new opportunities to assess choroidal changes in diabetic patients, the role of OCTA in early diagnosis and monitoring of diabetic choroidopathy remains unclear.

Objective
This review aims to evaluate the potential role of OCTA in diagnosing and monitoring diabetic choroidopathy.

Methods
A systematic review was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. Databases including PubMed, Embase, Cochrane Library, Google Scholar, ISI, and Scopus were searched for studies on diabetic choroidopathy assessed by OCTA. Studies included were peer-reviewed, published in English, and excluded case reports, conference proceedings, and studies on treated DR patients. Two independent reviewers screened articles for eligibility based on predefined criteria.

Results
OCTA allows for non-invasive, high-resolution visualization of retinal and choroidal microvasculature, providing both qualitative and quantitative data. The majority of studies indicate a significant decrease in choroidal perfusion parameters in diabetic patients without DR compared to healthy controls. Conflicting evidence exists regarding the correlation between choriocapillaris flow reduction and DR severity. OCTA may also predict changes in visual function related to choroidal perfusion, though it cannot fully replace clinical examinations.

Conclusions
OCTA is a valuable tool for early detection and monitoring of diabetic choroidopathy. However, its role is limited by variability in findings and its inability to detect certain features of diabetic microangiopathy. Further studies are needed to clarify its clinical utility and standardize assessment methods.
Impact of delayed pelvic imaging on the staging of biochemical recurrence in prostate cancer patients using [18F]DCFPYL PET/CT: a retrospective evaluation
Authors: Rodríguez-Pajuelo Ana , Guerra-Gómez Miriam , Cuenca Cuenca Juan Ignacio , Freire-Macías José María , Jiménez-Hoyuela García José Manuel , Álvarez-Pérez Rosa María 
Published: January 2025

Abstract

Purpose
The aim of this study was to evaluate the added diagnostic value of additional second stage pelvic scanning as part of the [18F]DCFPYL PET/CT procedure in patients treated for prostate cancer (PCa) who have biochemical recurrence (BR).

Materials and methods
Consecutive patients with a diagnosis of PCa who underwent a dual-phase PSMA-PET scan between September 2022 and December 2023, were retrospectively included. We analyzed the number and maximum SUV (SUVmax) of lesions only in the pelvic region (prostate, locoregional lymph nodes and bone), based on PSMA-RADS version 2.0 and miTNM criteria. To assess the potential diagnostic benefit of additional delayed pelvic PET/CT imaging as part of the PSMA-PET procedure, the change in molecular TNM classification was evaluated after the procedure.

Results
Additional delayed pelvic PET/CT imaging as part of the PSMA-PET procedure resulted in a change in molecular TNM classification in 22 out of 136 patients (16.2%). The highest percentage change was obtained in the miN classification (14/22 patients), followed by the miT classification (7/22) and lastly miM (1/22). Moreover, we found that patients in whom delayed pelvic imaging resulted in a change in molecular TNM classification were significantly older and had a higher PSA level than those in whom delayed imaging did not provide additional information.

Conclusions
Pelvic delayed imaging in patients with biochemical recurrence of prostate cancer undergoing PET/CT with [18F]DCFPYL shows a non-negligible influence on patient staging, modifying the miTNM classification in 16.2% of cases, with pelvic lymphatic involvement benefiting the most from the dual study.
Optimizing the Trade-off Between Batching and Waiting: Subadditive Dispatching
Authors: Erazo Ignacio , Toriello Alejandro 
Published: January 2025

Trading off Waiting and Batching Many modern logistics and production systems face an inherent tension between the need to dispatch or process orders or jobs as quickly as possible and the economies of scale obtained by batching. In “Optimizing the Trade-off Between Batching and Waiting: Subadditive Dispatching,” Ignacio Erazo and Alejandro Toriello propose a model that captures this tension, study various methods to optimize it or generate high-quality heuristic solutions, and assess the methods’ computational performance.
SAFER AG – Risk Assessment, Data, Design Standards, and Regulation: Needs and Recommendations
Authors: Shutske John M. , Issa Salah F. , Johnson Trent , Khorsandi Farzaneh , Pate Michael L. , Gorucu Serap , Walsh Jean , Yoder Aaron M. , Dukes Ellie , Aby Guy R. , Versweyveld Jim 
Published: January 2025

HighlightsRisk assessment is required for new machine designs, including autonomous, robotic, and other highly automated agricultural equipment.Meaningful data is needed to inform many risk assessment processes, yet there is no historical incident data on novel machine forms that have never been widely deployed.The establishment of an exposure database could supply needed data for machine risk assessment with new machine forms.Engineering standards are a bedrock for safe design, including newly developed standards for highly automated and autonomous machines.Outreach and awareness of engineering design standards are needed for engineers, non-engineers, regulators, and students.Abstract. The 2022 SAFER AG workshop convened stakeholder groups that addressed emerging challenges related to safety, research needs, workforce implications, community-level impacts, and other issues connected to the development and eventual widespread deployment of autonomous and highly automated agricultural machines. This article summarizes the key findings, discussions, and recommendations of one of the workshop’s working groups that focused their efforts on risk assessment and the data needed to inform risk assessment processes during machine design, engineering design, safety standards, and regulatory efforts. Participants engaged in a consensus-building process to identify gaps in current knowledge and practice, particularly concerning new machine forms for which historical safety data is limited or nonexistent. The group recommended innovative solutions, including creating an exposure-based database and exposure definitions that could serve as a foundation for future risk assessment processes. Workshop participants emphasized the critical role of consensus engineering standards in ensuring the safe design of autonomous, robotic, and other highly automated equipment, with a clear sense that engineering standards must be considered early in the design phases. The group also recommended that any desired regulatory activity at various levels (local, state, national, and international) must consider the knowledge that is already embedded in pre-existing engineering standards with the hope that future regulation aligns with these standards and leverages the expertise and care that goes into the consensus standards development process. Recommendations for outreach and education efforts targeting small manufacturers, regulators, students in ABET-accredited engineering programs, and academia are also outlined. Keywords: Autonomous agricultural machinery, Engineering standards, Exposure data, Industry outreach, Risk assessment, Robotic safety, Safety regulations.
The Neglected Tropical Disease Non-Governmental Organization Network: The role of a global health network in an evolving global health landscape
Authors: Sankar Girija , Dolegui Arielle , Acharya Jemish 
Published: January 2025

The Neglected Tropical Diseases NGO Network (NNN) was established in 2009 to bring together non-governmental organizations (NGOs) working towards the control and elimination of neglected tropical diseases (NTDs). In response to the evolving landscape of global health and the increasing focus on equity, the NNN conducted a membership analysis in 2021. This analysis aimed to assess the network's composition and connectivity, revealing significant insights into its current structure and areas for improvement. Key findings from the analysis include the following: a majority of NNN members were based in high-income countries (HICs); 40% of the member organizations concentrated on a single NTD; the African region had nearly twice the number of member organizations supporting interventions in the region compared to other regions; and, members from HICs tend to be better networked within the NNN. These findings highlight the need for the NNN to enhance its inclusivity and representation, ensuring that voices from endemic countries are more prominently integrated into the network. By aligning with the NTD Road Map 2021–2030, the NNN aims to address these disparities and strengthen its commitment to global health equity. This analysis serves as a call to action for other global health networks to undertake similar self-assessments. Through transparency and critical reflection, the pursuit of global health equity can be advanced, ensuring that all regions and diseases receive appropriate attention and resources.
The mediating role of workplace milieu resources on the relationship between emotional intelligence and burnout among leaders in social care
Authors: Kozák Anna , Schutzmann Réka , Soltész-Várhelyi Klára , Albert Fruzsina 
Published: January 2025

Background
This study investigated the connection between emotional intelligence and burnout through the mediating role of workplace milieu resources (a sense of community and mutual trust between employees) among social care leaders in Hungary utilizing the Job Demand-Resources model as a reference. The study evaluated emotional intelligence across three dimensions: understanding our emotions, understanding others’ emotions, and positive emotional appraisal.

Methods
A cross-sectional and quantitative study was conducted from 11th April to 30th November 2019 targeting Hungarian social care leaders. Participants (N = 547) were recruited non-randomly trough a training organized for them. Data collection involved sociodemographic questions, the Assessing Emotions Scale and the Copenhagen Psychosocial Questionnaire. Descriptive statistics, Pearson’s correlation, and three saturated serial mediations (ML with percentile bootstrap) were implemented. During the mediations, one dimension of emotional intelligence was used as a predictor in each model with the sense of community and mutual trust as serial mediators, and burnout as the outcome.

Results
The results confirmed the role of the leader’s emotional intelligence in creating a supportive workplace atmosphere and its indirect effect on burnout through these workplace milieu resources, while its direct effect was found not to be substantial. Among the three aspects of emotional intelligence, positive appraisal had the largest effect on burnout.

Conclusions
This study suggests that emotional intelligence’ influence on burnout is mediated by workplace milieu resources. Therefore, it is crucial to encourage leaders to use their emotional intelligence to create a positive emotional atmosphere rather than solely concentrating on emotional comprehension.
The drivers of mental health service utilisation among public primary healthcare workers in Negeri Sembilan
Authors: Mohd Noor Norhafizah , Ariffin Ahmad Azuhairi , Minhat Halimatus Sakdiah , Ying Lim Poh , Silim Umi Adzlin 
Published: January 2025

Background
Public primary healthcare workers (HCWs) face various psychosocial risks at workplace that can impact their mental health. However, little is known about their mental health service utilisation (MHSU). This study aimed to determine prevalence and predictors of MHSU among public primary HCWs in Negeri Sembilan, using Anderson Behavioural Model of Health Service Use.

Methods
A cross-sectional study was conducted from December 2022 to April 2023, using a valid and reliable self-administered six sections questionnaire consisting of; (i) sociodemographic, (ii) work-related factors, (iii) MHSU, (iv) perception of stigmatisation by others, (v) enabling factors, and (vi) need factors. Respondents were selected through proportionate stratified random sampling based on job categories. Multiple Logistic Regression using SPSS version 26 was used to determine the predictors of MHSU.

Results
A total of 294 respondents participated in this study, with a response rate of 83.5%. The 12-months MHSU prevalence was 45.6%. Mental health services were predominantly utilised for screening (96.3%) and treatment purposes (28.4%), primarily accessed through health clinics (85.1%), and interaction with paramedics (44.0%) and medical officers (38.8%). Significant drivers predicting MHSU were B40 household income (aOR = 3.426, 95% CI: 1.588, 7.393, p-value = 0.002) and M40 household income (aOR = 3.781, 95% CI: 1.916, 7.460, p-value<0.001), low supervisor support (aOR = 2.302, 95% CI: 1.206, 4.392, p-value = 0.011), received mental health training (aOR = 2.058, 95% CI: 1.221, 3.469, p-value = 0.007) and high co-worker support (aOR = 1.701, 95% CI: 1.034, 2.798, p-value = 0.036).

Conclusion
Almost half of respondents used mental health services, predicted by lower and middle household income, received mental health training and high co-worker support. Conversely, MHSU was also predicted by low supervisor support. To sustain high levels of MHSU, it is essential to implement regular mental health training targeted HCWs with lower to middle household incomes and those experiencing low supervisor support, while simultaneously enhancing co-worker support and screening program for early detection of mental health problems.
Scutellarin combined with lidocaine exerts antineoplastic effect in human glioma associated with repression of epidermal growth factor receptor signaling
Authors: He Xiu-Ying , Yang Yui-Si , Zheng Yue-Xiang , Xia Qing-Jie , Yu Hong-Zhou , Zhao Xiao-Ming , Wang Ting-Hua 
Published: January 2025

Purpose
Glioma is the most common primary intracranial tumors. Although great achievements have been made in the treatment, the efficacy is still unsatisfactory, which imposes a hefty burden on patients and society. Therefore, the exploration of new and effective anti-glioma drugs is urgent.

Methods
Human glioma cell lines U251 and LN229 were included in the study. Cell proliferation was detected by cell counting kit-8 (CCK8), plate clone formation assay, EdU incorporation assay and xCELLigence real-time cell analyzer. Cell apoptosis was evaluated by TUNEL assay and flow cytometry. Then, transwell assay was used for assessing the migration. Moreover, tumor xenograft model was established to examine the effect of scutellarin (SCU) and lidocaine on the growth of glioma in vivo. Lastly, western blot was performed to detect the protein level of epidermal growth factor receptor (EGFR).

Results
In present study, we found that SCU and lidocaine suppressed the proliferation and migration, and induced the apoptosis of human glioma cell lines, including U251 and LN229 cells, in a dose-dependent manner in vitro. Moreover, the combination of SCU and lidocaine further restrained the proliferation and migration ability of U251 and LN229 cells, while induced their apoptosis in vitro. Additionally, SCU and lidocaine also inhibited the growth of glioma in vivo, and the effect of the combination was better. Above all, the toxicity of SCU and its combination with lidocaine was low to normal astrocytes and neurons. Mechanistically, the effect of SCU and its combination with lidocaine on glioma cells was partially associated with the repression of EGFR signaling.

Conclusions
Scutellarin and lidocaine exerted a synergistic effect on suppressing the proliferation and migration and inducing the apoptosis of glioma cells, which was partly associated with the repression of EGFR signaling.