bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rice bran phytosterol nanoparticles: A promising approach to enhance yogurt quality and nutrition
Authors: Feng Simin , Guo Baihui , Wang Liling , Sui Minghui , Shao Ping 
Published: December 2023

AbstractThis study aimed to improve the comprehensive utilization value of rice bran by investigating catalysts for the methyl esterification reaction of rice bran oil deodorized distillates and preparing phytosterol nanoparticles for addition to yogurt. Fe2(SO4)3 was found to be an effective catalyst, achieving a methyl esterification rate of 98.07 ± 0.23% under optimal conditions. Then, phytosterol nanoparticles were prepared and added to yogurt, resulting in stable addition with the pH decreased from 4.23 ± 0.01 to 4.02 ± 0.01 and the titratable acidity increased from 106.48 ± 0.85 °T to 117.07 ± 0.82 °T during storage. The addition of phytosterol nanoparticles increased the apparent viscosity from 0.68 ± 0.01 Pa s to 0.72 ± 0.02 Pa s and the G* from 80.01 ± 5.50 Pa to 91.80 ± 1.99 Pa, resulting in thicker and more elastic texture. Phytosterol nanoparticle improves the dispersion and stability of phytosterols in yogurt, thus making it stable to be added to yogurt. Fe2(SO4)3 is a suitable catalyst for the methyl esterification reaction of rice bran oil deodorized distillates, and the addition of rice bran phytosterol nanoparticles to yogurt can enhance its texture and nutritional value, offering a promising strategy for producing high value‐added products from rice bran.
Environmental DNA as a Tool for Better Understanding the Distribution, Abundance, And Health of Atlantic Salmon and Pacific Salmon
Authors: Ramey Andrew M , McKeeman Cherie M , Petrou Eleni L , Menning Damian M , Russ Ora L , López J Andrés 
Published: December 2023

Abstract
The development and application of approaches to detect and quantify environmental DNA (eDNA) have potential to improve our understanding of the distribution, abundance, and health of Atlantic Salmon Salmo salar and Pacific salmon Oncorhynchus spp. Here, we review 61 articles focusing on eDNA applications pertaining to salmon occupying natural habitat and aquaculture facilities in the context of advances, opportunities, and challenges. Given recent advances, eDNA now serves as a useful tool for detecting Atlantic Salmon and Pacific salmon and understanding threats to the health of fish and their habitats. Opportunities exist to apply sensitive and minimally invasive eDNA approaches to detect fish and assess fish habitat, assess range expansions of salmon and salmon pathogens, and detect invasive species that may threaten salmon health and abundance. Near real-time eDNA detection and quantification approaches to inform fisheries management may be on the horizon. Challenges limiting the widespread application of eDNA approaches for informing salmon management include accounting for the many factors affecting detection and quantification of eDNA, limits of data for deriving inference, and expense. Through continued development and refinement, eDNA approaches are anticipated to become increasingly available to, and utilized by, managers of Atlantic Salmon and Pacific salmon fisheries.
Green hydrogen production by intermediate‐temperature protonic solid oxide electrolysis cells: Advances, challenges, and perspectives
Authors: Tang Chunmei , Yao Yao , Wang Ning , Zhang Xiaohan , Zheng Fangyuan , Du Lei , Luo Dongxiang , Aoki Yoshitaka , Ye Siyu 
Published: December 2023

AbstractProtonic solid oxide electrolysis cells (P‐SOECs) operating at intermediate temperatures, which have low costs, low environmental impact, and high theoretical electrolysis efficiency, are considered promising next‐generation energy conversion devices for green hydrogen production. However, the developments and applications of P‐SOECs are restricted by numerous material‐ and interface‐related issues, including carrier mismatch between the anode and electrolyte, current leakage in the electrolyte, poor interfacial contact, and chemical stability. Over the past few decades, considerable attempts have been made to address these issues by improving the properties of P‐SOECs. This review comprehensively explores the recent advances in the mechanisms governing steam electrolysis in P‐SOECs, optimization strategies, specially designed components, electrochemical performance, and durability. In particular, given that the lack of suitable anode materials has significantly impeded P‐SOEC development, the relationships between the transferred carriers and the cell performance, reaction models, and surface decoration approaches are meticulously probed. Finally, the challenges hindering P‐SOEC development are discussed and recommendations for future research directions, including theoretical calculations and simulations, structural modification approaches, and large‐scale single‐cell fabrication, are proposed to stimulate research on P‐SOECs and thereby realize efficient electricity‐to‐hydrogen conversion.image
Effect of gamma irradiation and supercritical carbon dioxide sterilization with Novakill™ or ethanol on the fracture toughness of cortical bone
Authors: Shin Mihee , Pelletier Matthew H. , Lovric Vedran , Walsh William R. , Martens Penny J. , Kruzic Jamie J. , Gludovatz Bernd 
Published: December 2023

AbstractSterilization of structural bone allografts is a critical process prior to their clinical use in large cortical bone defects. Gamma irradiation protocols are known to affect tissue integrity in a dose dependent manner. Alternative sterilization treatments, such as supercritical carbon dioxide (SCCO2), are gaining popularity due to advantages such as minimal exposure to denaturants, the lack of toxic residues, superior tissue penetration, and minor impacts on mechanical properties including strength and stiffness. The impact of SCCO2 on the fracture toughness of bone tissue, however, remains unknown. Here, we evaluate crack initiation and growth toughness after 2, 6, and 24 h SCCO2‐treatment using Novakill™ and ethanol as additives on ~11 samples per group obtained from a pair of femur diaphyses of a canine. All mechanical testing was performed at ambient air after 24 h soaking in Hanks' balanced salt solution (HBSS). Results show no statistically significant difference in the failure characteristics of the Novakill™‐treated groups whereas crack growth toughness after 6 and 24 h of treatment with ethanol significantly increases by 37% (p = .010) and 34% (p = .038), respectively, compared to an untreated control group. In contrast, standard 25 kGy gamma irradiation causes significantly reduced crack growth resistance by 40% (p = .007) compared to untreated bone. FTIR vibrational spectroscopy, conducted after testing, reveals a consistent trend of statistically significant differences (p < .001) with fracture toughness. These trends align with variations in the ratios of enzymatic mature to immature crosslinks in the collagen structure, suggesting a potential association with fracture toughness. Additional Raman spectroscopy after testing shows a similar trend with statistically significant differences (p < .005), which further supports that collagen structural changes occur in the SCF‐treated groups with ethanol after 6 and 24 h. Our work reveals the benefits of SCCO2 sterilization compared to gamma irradiation.
MiR‐600 mediates EZH2/RUNX3 signal axis to modulate breast cancer cell viability and sorafenib sensitivity
Authors: Zhao Qian , Li Dan , Feng Jinchun , Jinsihan Dilixiati 
Published: December 2023

AbstractBreast cancer (BC) ranks as the most prevalent gynecologic tumor globally. Abnormal expression of miRNAs is concerned with the development of cancers such as BC. However, little is known about the role of miR‐600 in BC. This work aimed to explore the role of miR‐600 in the malignant progression and sorafenib sensitivity of BC cells. Expression and interaction of miR‐600/EZH2/RUNX3 were analyzed by bioinformatics. qRT‐PCR was utilized to assay RNA expression of miR‐600 and mRNA expression of EZH2/RUNX3. The binding relationship between miR‐600 and EZH2 was tested by dual luciferase assay and RNA immunoprecipitation (RIP). The effects of miR‐600/EZH2/RUNX3 axis on the malignant behavior and sorafenib sensitivity of BC cells were detected by CCK‐8 and colony formation assay. Low expression of miR‐600 and RUNX3 in BC was found by bioinformatics and molecular assays. High expression of EZH2 in BC was negatively correlated with RUVX3. Dual luciferase assay and RIP demonstrated that MiR‐600 could bind to EZH2. Cell assays displayed that miR‐600 knockdown could foster the malignant progression of BC cells and reduce the sensitivity of BC cells to sorafenib. EZH2 knockdown or RUNX3 overexpression could offset the effect of miR‐600 inhibitor on the malignant behavior and sorafenib sensitivity of BC cells. MiR‐600 can hinder the malignant behavior of BC cells and foster sensitivity of BC cells to sorafenib via EZH2/RUNX3 axis, exhibiting the miR‐600/EZH2/RUNX3 axis as a feasible therapeutic target for BC patients.
Lixisenatide ameliorated lipopolysaccharide (LPS)‐induced expression of mucin and inflammation in bronchial epithelial cells
Authors: Xu Leiming , Chen Guoping , Zhang Leiming , He Aifeng , Li Yong 
Published: December 2023

AbstractChronic obstructive pulmonary disease (COPD) induces serious social and economic burdens due to its high disability and mortality, the pathogenesis of which is highly involved with inflammation, oxidative stress (OS), and mechanism of mucin 5AC (MUC5AC) secretion. Lixisenatide is a selective glucagon‐like peptide 1 receptor agonist recently reported to have anti‐inflammatory properties. Our study will focus on the potential impact of lixisenatide on lipopolysaccharide (LPS)‐induced mucin secretion and inflammation in 16 human bronchial epithelial (16HBE) cells to check its potential function in COPD. 16HBE cells were treated with LPS, with or without lixisenatide (10 and 20 nM) for 1 day. Remarkably declined cell viability, enhanced lactate dehydrogenase release, activated OS, and elevated release of inflammatory cytokines were observed in LPS‐treated 16HBE cells, accompanied by the activation of nuclear factor‐κB signaling, all of which were signally reversed by lixisenatide. Moreover, elevated expression and release of MUC5AC were observed in LPS‐treated 16HBE cells but were markedly repressed by lixisenatide. Furthermore, the repressed nuclear factor erythroid 2‐related factor 2 (Nrf2) level in LPS‐treated 16HBE cells was notably rescued by lixisenatide. Lastly, following the knockdown of Nrf2, the protective function of lixisenatide on LPS‐triggered MUC5AC release in 16HBE cells was significantly abrogated. Collectively, lixisenatide ameliorated LPS‐induced expression of mucin and inflammation in bronchial epithelial cells by regulating Nrf2.
Predicting the catalytic activities of transition metal (Cr, Fe, Co, Ni) complexes towards ethylene polymerization by machine learning
Authors: Meraz Md Mostakim , Yang Wenhong , Yang Weisheng , Sun Wen‐Hua 
Published: December 2023

AbstractThe study aims to execute machine learning (ML) method for building an intelligent prediction system for catalytic activities of a relatively big dataset of 1056 transition metal complex precatalysts in ethylene polymerization. Among 14 different algorithms, the CatBoost ensemble model provides the best prediction with the correlation coefficient (R2) values of 0.999 for training set and 0.834 for external test set. The interpretation of the obtained model indicates that the catalytic activity is highly correlated with number of atom, conjugated degree in the ligand framework, and charge distributions. Correspondingly, 10 novel complexes are designed and predicted with higher catalytic activities. This work shows the potential application of the ML method as a high‐precision tool for designing advanced catalysts for ethylene polymerization.
Validation of a good manufacturing practice procedure for the production of [11C]AZD4747, a CNS penetrant KRASG12c inhibitor
Authors: Cortés González Miguel A. , Högnäsbacka Antonia A. , Halldin Christer , Schou Magnus 
Published: December 2023

AZD4747 is a KRASG12C inhibitor recently shown to cross the non‐human primate blood‐brain barrier efficiently. In the current study, a GMP‐compliant production of [11C]AZD4747 was developed to enable PET studies in human subjects. The validated procedure afforded [11C]AZD4747 as an injectable solution in good radioactivity yield (1656 ± 532 MBq), excellent radiochemical purity (100%), and a molar activity of 77 ± 13 GBq/μmol at the end of the synthesis, which took 46 ± 1 min from the end of the bombardment. Quality control on the final product was performed satisfactorily and met all acceptance criteria.
SSR1andCKAP4as potential biomarkers for intervertebral disc degeneration based on integrated bioinformatics analysis
Authors: Guo Danqing , Zeng Min , Yu Miao , Shang Jingjing , Lin Jinxing , Liu Lichu , Yang Kuangyang , Cao Zhenglin 
Published: December 2023

AbstractBackgroundIntervertebral disc degeneration (IDD) is a significant cause of low back pain and poses a significant public health concern. Genetic factors play a crucial role in IDD, highlighting the need for a better understanding of the underlying mechanisms.AimThe aim of this study was to identify potential IDD‐related biomarkers using a comprehensive bioinformatics approach and validate them in vitro.Materials and MethodsIn this study, we employed several analytical approaches to identify the key genes involved in IDD. We utilized weighted gene coexpression network analysis (WGCNA), MCODE, LASSO algorithms, and ROC curves to identify the key genes. Additionally, immune infiltrating analysis and a single‐cell sequencing dataset were utilized to further explore the characteristics of the key genes. Finally, we conducted in vitro experiments on human disc tissues to validate the significance of these key genes in IDD.Resultswe obtained gene expression profiles from the GEO database (GSE23130 and GSE15227) and identified 1015 DEGs associated with IDD. Using WGCNA, we identified the blue module as significantly related to IDD. Among the DEGs, we identified 47 hub genes that overlapped with the genes in the blue module, based on criteria of |logFC| ≥ 2.0 andp.adj <0.05. Further analysis using both MCODE and LASSO algorithms enabled us to identify five key genes, of which CKAP4 and SSR1 were validated by GSE70362, demonstrating significant diagnostic value for IDD. Additionally, immune infiltrating analysis revealed that monocytes were significantly correlated with the two key genes. We also analyzed a single‐cell sequencing dataset, GSE199866, which showed that both CKAP4 and SSR1 were highly expressed in fibrocartilage chondrocytes. Finally, we validated our findings in vitro by performing real time polymerase chain reaction (RT‐PCR) and immunohistochemistry (IHC) on 30 human disc samples. Our results showed that CKAP4 and SSR1 were upregulated in degenerated disc samples. Taken together, our findings suggest that CKAP4 and SSR1 have the potential to serve as disease biomarkers for IDD.
Radiological Versus Clinical 1‐Year Outcomes of Dupilumab in Refractory CRSwNP: A Real‐Life Study
Authors: Giombi Francesco , Pace Gian Marco , Nappi Emanuele , Giunta Gianmarco , Muci Giovanna , Pirola Francesca , Ferreli Fabio , Heffler Enrico , Paoletti Giovanni , Giannitto Caterina , Mercante Giuseppe , Francone Marco , Spriano Giuseppe , Canonica Giorgio Walter , Malvezzi Luca 
Published: December 2023

ObjectiveTo provide real‐life evidence on long‐term radiological changes in patients with severe chronic rhinosinusitis with nasal polyps (CRSwNP) treated with dupilumab, and to assess possible differences between radiological and clinical results in terms of endoscopic findings and Patient‐Reported‐Outcomes (PROs).MethodsConsecutive patients treated with dupilumab for recalcitrant CRSwNP were required to undergo CT scan at baseline (T0) and after 12 (T1) since first administration. A group of patients also performed CT scan at 52 weeks (T2) to assess long‐term outcomes. At each timepoint, patients underwent nasal endoscopy, assessment of Nasal‐Polyp‐Score (NPS), Lund‐Kennedy‐Score (LKS), and had to fill in the 22‐item Sinonasal‐Outcome‐Test (SNOT‐22) and Visual‐Analogue‐Scales (VAS) for sinonasal symptoms.ResultsIn fifty‐three included patients, from T0 to T1 we detected a significant reduction in mean Lund‐Mackay score (LM), PROs (SNOT‐22, VAS) and endoscopic (NPS, LKS) scores (p < 0.05). In the subset of patients that reached T2 (n = 30), compared to T1, we observed a further significant decrease in mean LM, SNOT‐22, VAS, and NPS scores, but not in LKS (p = 0.420). At T1, the highest improvement was observed in PROs (SNOT‐22: 56.26%), and polyp size (NPS: 49.83%). Conversely, between T1 and T2, sinus opacification was shown to be the most improved outcome (LM: 36.86%).ConclusionsOur experience showed that poorly controlled CRSwNP patients treated with dupilumab experienced significant improvement in radiologic, endoscopic and clinical disease severity. While in the initial 3 months, PROs garnered attention for showing earlier effectiveness, radiological outcomes revealed sustained and gradual efficacy in a longer term.Level of EvidenceLevel 4. According to the Oxford Center for Evidence‐Based Medicine 2011 level of evidence guidelines, this non‐randomized retrospective cohort study is classified as level 4 evidence Laryngoscope, 134:2626–2633, 2024
Zoning management framework for comprehensive land consolidation in oasis rural in arid area: Case study of the Ugan‐Kuqa River Delta Oasis in Xinjiang, China
Authors: Ma Chen , Li Manchun , Wang Hongwei , Jiang Penghui , Luo Kui 
Published: December 2023

AbstractComprehensive land consolidation (CLC) is a strategy to promote the intensive, efficient, and sustainable use of land, and is of great significance in repairing degraded land in oases, reversing the decline of the countryside, and activating multiple values of land use. However, how to scientifically assess and zoning management of CLC in dryland oases remains an open question. In this study, a comprehensive analytical framework was established to assess the characteristics of land‐use transformation and the potential for CLC using remotely sensed and geospatial data and spatial analysis methods, a multi‐objective territorial differentiation model, and collaborative path for CLC in oasis villages was proposed. The following key results were obtained. (1) From 2009 to 2018, urban and rural settlements in the hinterland of the Weiku Oasis expanded by 40 km2, and shrub forests and grasslands in the oasis‐desert transition zone shrank substantially by 21.13 km2. The newly cultivated farmland in the oasis‐desert transition zone only offset 62.9% of the occupied farmland in the hinterland of the oasis. (2) The pattern of comprehensive rural land consolidation potential in the Weiku Oasis was consistent with territorial differentiation, with a gradient decrease from the hinterland of the oasis to the desert. The average potential for Comprehensive land consolidation (PCLC) index was 0.31. (3) Three consolidation priorities and six consolidation types were identified for the proposal of 14 combined modes of CLC. Territorial differentiation with respect to the combined modes was discussed. Overall, this study provides useful insights for promoting efficient and sustainable utilization of oasis land resources and implementing zoning management.
Stop and smell the roses: How and when busyness impacts consumers' preference for minimalist advertisements
Authors: Chen Siyun , Ponomarenko Veronika , Xiao Tingwen , Lv Linxiang , Liu Guanrong 
Published: December 2023

AbstractIn an era marked by the rapid growth of digital media and increasingly limited attention spans, advertisements employing minimalist aesthetics have emerged as a compelling approach. Yet, the specific conditions under which consumers favor minimalist ads remain underexplored. Through a comprehensive series of studies, including a field study and five scenario‐based experiments, this research unveils new insights into how busyness shapes consumer preferences for minimalist ad designs. This research found that busyness notably steers preferences toward minimalist over complex ads, both in real‐world (Study 1) and experimental environments (Study 2). Notably, we revealed a serial mediation, with busyness affecting ad preference through the desire for relaxation (distal mediator) followed by the intent to avoid processing disfluency (proximal mediator) (Study 3). Furthermore, two boundary conditions were identified in which this effect is attenuated or intensified. When consumers place a high (vs. low) price on time, the busyness effect on minimalism preference is undermined (Study 4). In addition, this effect is more pronounced when a temporal landmark that designates an end (vs. start) is made salient (Studies 5 and 6). These insights not only illuminate consumer behavior in visual marketing but also carry significant practical implications for advertisers, marketing professionals, and policymakers.
Drugs/agents for the treatment of ischemic stroke: Advances and perspectives
Authors: Jia Jian , Jiao Weijie , Wang Guan , Wu Jianbing , Huang Zhangjian , Zhang Yihua 
Published: December 2023

AbstractIschemic stroke (IS) poses a significant threat to global human health and life. In recent decades, we have witnessed unprecedented progresses against IS, including thrombolysis, thrombectomy, and a few medicines that can assist in reopening the blocked brain vessels or serve as standalone treatments for patients who are not eligible for thrombolysis/thrombectomy therapies. However, the narrow time windows of thrombolysis/thrombectomy, coupled with the risk of hemorrhagic transformation, as well as the lack of highly effective and safe medications, continue to present big challenges in the acute treatment and long‐term recovery of IS. In the past 3 years, several excellent articles have reviewed pathophysiology of IS and therapeutic medicines for the treatment of IS based on the pathophysiology. Regretfully, there is no comprehensive overview to summarize all categories of anti‐IS drugs/agents designed and synthesized based on molecular mechanisms of IS pathophysiology. From medicinal chemistry view of point, this article reviews a multitude of anti‐IS drugs/agents, including small molecule compounds, natural products, peptides, and others, which have been developed based on the molecular mechanism of IS pathophysiology, such as excitotoxicity, oxidative/nitrosative stresses, cell death pathways, and neuroinflammation, and so forth. In addition, several emerging medicines and strategies, including nanomedicines, stem cell therapy and noncoding RNAs, which recently appeared for the treatment of IS, are shortly introduced. Finally, the perspectives on the associated challenges and future directions of anti‐IS drugs/agents are briefly provided to move the field forward.
Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish
Authors: Divyanshi  , Yang Jing 
Published: December 2023

AbstractSpecification of the germline and its segregation from the soma mark one of the most crucial events in the lifetime of an organism. In different organisms, this specification can occur through either inheritance or inductive mechanisms. In species such as Xenopus and zebrafish, the specification of primordial germ cells relies on the inheritance of maternal germline determinants that are synthesized and sequestered in the germ plasm during oogenesis. In this review, we discuss the formation of the germ plasm, how germline determinants are recruited into the germ plasm during oogenesis, and the dynamics of the germ plasm during oogenesis and early embryonic development.