bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced Multi-Stage Guided-Attention Mechanism for Traffic Accident-Based Patient Safety Prediction Model
Authors: Baswaraju Swathi , Kamala S. Praveena Rachel , E Naresh , Pareek Piyush Kumar 
Published: November 2023

Abstract
The patient safety prediction model is required for analysis by considering the traffic accident database. In this paper, develop an Enhanced Multi Stage Guided Attention Mechanism (EMSGAM) for a traffic accident-based patient safety prediction model. Patient safety is analyzed by traffic accident data. This data is containing various parameters of age, gender, BMI, hypertension, diabetes, deficiency, depression, and so on. The Min-Max normalisation method is first used to normalise the dataset. Then, with two classes of patients—low-risk and high-risk—the gathered database is used to predict patient safety. The pre-processed data is then forwarded to the feature selection stage, where the necessary features are picked out of the input features. Correlation-based feature selection (CFS) is used to pick the features. The Multi Stage Guided Attention Mechanism (MSGAM) receives the data in order to classify it for patient safety. Long Short-Term Memory (LSTM), the self-attention mechanism, and the Variational Autoencoder (VAE) model are all combined to create the proposed classifier. In this architecture, the optimal weighting parameter is chosen by using Improved Tasmanian Devil Optimization (ITDO). The optimization process is enhancing the performance of the classifier. Based on this evaluation, patient safety is analyzed by considering traffic accident data. This dataset is considered for analysing low-risk and high-risk patients. Performance is assessed using performance measurements, and the suggested is implemented in MATLAB.
Study on heat transfer performance of cold plate with grid channel
Authors: Zhang Kaihua , Ye Xiaojiang , Hou Zhijian , Ren Yahui , Shi Qiuyi 
Published: November 2023

Abstract
The utilization of cold plate radiators as a prevalent method for indirect liquid cooling has been extensively investigated and implemented in server cooling systems. However, there is a lack of comprehensive study on the application of this technology at the chip size, indicating a need for more development and exploration in this area. A proposal was made for a grid-channel chip cold plate heat sink to facilitate the dissipation of heat from a chip. tests were conducted to investigate the impact of the flow rate of the cold plate and the layout of the inlet and outlet on various thermal parameters, including the average temperature, maximum temperature, thermal resistance, and uniformity coefficient of the cold plate. The tests were specifically conducted under a chip power of 150W, and the accuracy of the simulation was confirmed through the use of FLUENT. The findings indicate that the cold plate effectively regulates the temperature of the chip, ensuring it remains below 85℃ throughout all experimental groups. In contrast to the single in single out (SISO) configuration, the single-in multiple-out (SIMO) layout exhibits a higher degree of temperature uniformity within the cold plate. Nevertheless, it is important to note that augmenting the quantity of exits does not guarantee an improvement in heat transfer efficiency. This outcome is contingent upon the presence of a longitudinal flow channel shared by the outlet and intake, as well as the dispersion characteristics of the outlet. Enhancing the dispersion of the exit can significantly enhance the thermal transfer efficiency of the cold plate.
Short-term practice of deficit irrigation and nutrition influences soil–root interface, and water use efficiency of winter fodder corn in semi-arid region
Authors: Halli Hanamant M. , Govindasamy Prabhu , E Senthamil , G Vinay M , Wasnik Vinod , Ullah Fazal , Yadav Vijay , Kumar B. N. Aravinda , Elansary Hosam O. , S Basavaraj P , M Boraiah K , B Harisha C 
Published: November 2023

Abstract
Water scarcity is a global concern due to climate change induced irregular rainfall, and unsustainable water management practices. This study assessed the interaction of deficit irrigation and nutrient levels on soil-roots interface, water use efficiency (WUE), and seed yield in winter fodder corn. Three-year experiment (2018–2021) in Central India consists three irrigation levels (I1, 25% soil moisture depletion, full irrigation; I2, 50% depletion; I3, 75% depletion, severe deficit) and four nutrient rates (N1, no NPK; N2, N 100, P 40 kg ha–1; N3, N 120, P 60, K 40 kg ha–1; N4, N3 + Zinc sulphate 20 kg ha–1), in a split-plot design with three replications. Moderate deficit irrigation (I2) with higher nutrient rates (N4) favored corn root growth by decreasing soil bulk density (by 2.2%), while increasing soil carbon stock (11.19 Mg ha−1) and available nutrients (N, P, K) in the upper (0–15 cm) soil layer. This combination enhanced root growth, resulted in longer roots (20.65–23.09%), with higher volume (43.44–69.88%), surface area (41.65–43.90%), number of crown roots (31.84–53.36%), and dry weight (34.61–42.81%). Additionally, I2 × N4 improved WUE (36.32%), seed yield (39.09%) and stover yield (21.20%) allowing for 26.08% water conservation, while increasing soil organic carbon (by 11.19%) compared to full and severe deficit irrigation. Moderate deficit irrigation with optimum nutrients offers a viable strategy to augment WUE and seed yield in fodder corn for water-scarce regions. The study underscores the detrimental effects of excessive irrigation on soil and crop roots.
Systematic review and meta-analysis of Hepatitis E prevalence in Southeast Asia: A comprehensive assessment of epidemiological patterns
Authors: Mirzaev Ulugbek Khudayberdievich , Ouoba Serge , Ko Ko , Phyo Zayar , Chhoung Chanroth , Ataa Akuffo Golda , Sugiyama Aya , Akita Tomoyuki , Tanaka Junko 
Published: November 2023

Abstract
The burden of hepatitis E in Southeast Asia is substantial, influenced by its distinct socio-economic and environmental factors, as well as variations in healthcare systems. The aim of this study was to assess the pooled prevalence of hepatitis E across countries within the Southeast Asian region by the UN division. A comprehensive systematic database search was conducted across PubMed, Web of Science, and Scopus databases, supplemented by a manual search in Google Scholar. Studies that presented seroprevalence data of hepatitis E virus (HEV) in Southeast Asian nations were eligible for inclusion, and a meta-analysis was subsequently conducted. The assessment of bias risk was undertaken using the Joanna Briggs Institute checklist. The ensuing meta-analysis provides an evaluation of 66 chosen studies from a pool of 1641, encompassing a total cohort of 44,850 individuals from Southeast Asian countries. The pooled prevalence of anti-HEV IgG within Southeast Asian countries was determined to be 21.03%. Stratification of the study population based on the risk of HEV acquisition into two cohorts—high-risk and low-risk groups—yielded a pooled anti-HEV IgG prevalence of 28.9%, with an accompanying IgM prevalence of 4.42% in the former group, while the latter group exhibited figures of 17.86% and 3.15%, respectively. A temporal analysis, segmented into three distinct decades (1987–1999, 2000–2010, 2011–2023), indicated an uprising trend in both IgG and IgM prevalence over the specified time spans. These findings contribute to a better understanding of HEV prevalence across populations and time periods in Southeast Asia, shedding light on important public health implications and suggesting directions for further research and intervention strategies.
Coal-Rock Crack Image Segmentation Based on Attention Mechanism and Multi-Scale Features
Authors: Jia Haixin , Chen Zhaoxuan , Zhang Guoying , Liu Wenzhuo , Zhang Zirui 
Published: November 2023

Abstract
Global contextual information and local information are crucial for the segmentation of coal rock fractures. For global information, most existing schemes directly input the feature maps output by CNN into Transformer for extraction. Due to the limitation of feature map size, Transformer is unable to further extract multi-scale information of cracks, making it unsuitable for cracks of different sizes; Moreover, the feature map contains data with low correlation with cracks, and directly inputting it into Transformer will affect the extraction of global information about cracks.Unet based networks are one of the mainstream solutions for extracting local detail information in images, but the low-level features of their skip connections contain redundant information, which directly leads to false positive segmentation results.Based on the above issues, this article designs the Channel Attention Atrus Spatial Pyramid Pooling module (TD-ASPP) and the Cross Attention Non-Local Block (CANB), and integrates them into Transunet to propose a coal rock fracture image segmentation based on attention mechanism and multi-scale features (MS-Unet).Firstly, the TD-ASPP module is used to capture multi-scale features of cracks, while strengthening channels with strong correlation with cracks and diluting channels with weak correlation, in order to further improve the accuracy of the transformer in calculating global information of cracks. Secondly, the CANB module can utilize advanced features to supervise and strengthen low-level features, further enhancing local details of cracks and suppressing false positives.
Sequence-Specific Recognition of Double-Stranded Nucleic Acids by λ Exonuclease-Phosphorylated DNA Mediated Target Interrogation
Authors: Su Xin , Fu Shengnan , Li Junjie , Chen Jing , Zhang Linghao , Liu Jiajia 
Published: November 2023

Abstract

The CRISPR-Cas system, guided by an RNA molecule, cleaves double-stranded DNA targets in proximity to a protospacer adjacent motif (PAM), enabling the applications in genetic editing/interference and molecular diagnostics1–3. However, the reliance on PAM-dependent recognition and cleavage has limited the range of targetable sequences and led to undesired off-target effects, resulting in false activations4–7. Here, we conducted single-molecule FRET (smFRET) analysis and discovered a remarkable enzyme activity of λ Exonuclease (λ Exo)8. This activity involves the binding of 5’-phosphorylated single-stranded DNA (pDNA) to double-stranded (ds-) DNA and DNA-RNA duplexes, without the need for a specific PAM-like motif. Upon binding to its complementary region on nucleic acid targets, the λ Exo-pDNA system catalytically digests the pDNA into nucleotides in the presence of Mg2+. Importantly, this process is sensitive to mismatches, resulting in exceptional sequence specificity and greatly reduced off-target effects. Leveraging this unique property, we successfully demonstrated various applications, such as molecular diagnostics, synthetic DNA circuits, and in situ genomic imaging. Direct recognition of ds- nucleic acid was achieved by simple fluorescence reporting. DNA circuits9,10 with ds- nucleic acid inputs were established to execute the logical operation and signal amplification. The λ Exo-pDNA system enabled in situ imaging of genomic loci in fixed cells, directly observing both high-copy loci (e.g., telomeres) and low-copy loci (e.g., MUC-4). The non-trivial activity of λ Exo has unlocked PAM-independent target recognition and improved sequence specificity and accuracy. This discovery holds immense promise for advancing gene regulation and molecular diagnostics in the future.
Where you begin is not necessarily where you end: the mental and physical health trajectories of cancer caregivers over time
Authors: Wilson-Genderson Maureen , Thomson Maria D , Siminoff Laura A 
Published: November 2023

Abstract
Purpose
Cancer caregiving, a critical component in the cancer-care model, has deleterious effects on the caregiver’s physical and mental health. The degree to which these negative effects are uniformly experienced by caregivers is unclear. The impact of the secondary caregiver’s absence on the primary caregivers’ well-being is understudied.
Methods
Terminal cancer patient-caregiver dyads (n = 223) were recruited from oncology clinics and followed for six months or until patient death. Longitudinal latent growth models were used to characterize the heterogeneity of caregiver physical health and depressive symptoms; characteristics associated with these trajectories are examined.
Results
Caregivers were majority female (74%), white (55%) and patient spouses (60%). Two physical health (moderate, stable; initially good, declining) and two depressive symptom (moderate, stable; high, increasing) trajectories were identified. Declining physical health was more likely among caregivers who were healthiest at baseline, had higher levels of education, lower subjective burden, fewer depressive symptoms, cared for patients with fewer functional limitations and reported fewer caregiving tasks rendered by a secondary caregiver. Those with increasing depressive symptoms were more likely to be white, patient’s wife, have higher subjective caregiver burden, lower physical health, and care for a patient with greater functional limitations.
Conclusions
Decreasing physical health was evident among caregivers who were initially healthier and reported less assistance from secondary caregivers. Increasing depression was seen in white, female spouses with higher subjective burden. Sample heterogeneity revealed hidden groups unexpectedly at risk in the primary cancer caregiver role to which the oncology care team should be alert.
Enhanced Oxidative Stress Resistance in Ustilago maydis and Its Implications on the Virulence
Authors: Cuamatzi-Flores Jorge , Colón-González Maritrini , Requena-Romo Fernanda , Quiñones-Galeana Samuel , Cervantes-Chávez José Antonio , Morales Lucía 
Published: November 2023

Abstract
The phytopathogenic fungus Ustilago maydis causes corn smut by suppressing host plant defenses, including the oxidative burst response. While many studies have investigated how U. maydis responds to oxidative stress during infection, the consequences of heightened resistance to oxidative stress on virulence remain understudied. This study aimed to identify the effects on virulence in U. maydis strains exhibiting enhanced resistance to hydrogen peroxide (H2O2).
To achieve this, we exposed U. maydis SG200 to 20 escalating H2O2 shocks, resulting in an adapted strain resistant to concentrations as high as 60 mM of H2O2, a lethal dose for the initial strain. Genetic analysis of the adapted strain revealed five nucleotide substitutions, two minor copy number variants, and a large amplification event on chromosome nine (1-149 kb) encompassing the sole catalase gene. Overexpressing catalase increased resistance to H2O2; however, this resistance was lower than that observed in the adapted strain. Additionally, virulence was reduced in both strains with enhanced H2O2-resistance.
In summary, enhanced H2O2 resistance, achieved through either continuous exposure to the oxidative agent or through catalase overexpression, decreased virulence. This suggests that the response to the oxidative stress burst in U. maydis is optimal and that increasing the resistance to H2O2 does not translate into increased virulence. These findings illuminate the intricate relationship between oxidative stress resistance and virulence in U. maydis, offering insights into its infection mechanisms.
Unravelling the complexity of silk sericins: P150/sericin 6 is a new silk gene in Bombyx mori
Authors: Wu Bulah Chia-hsiang , Zabelina Valeriya , Zurovcova Martina , Zurovec Michal 
Published: November 2023

Abstract

Sericins are a small family of highly divergent proteins that serve as adhesives and coatings for silk fibers and are produced in the middle part of the silk gland. So far, five genes encoding sericin proteins have been found in Bombyx mori. Sericins 1 and 3 are responsible for silk adhesion in the cocoon, while sericins 2, 4, and 5 are present in non-cocoon spun silk of younger larvae (including the early last instar). We found a new gene, which we named P150/sericin 6, which appears to be an ortholog of the sericin-like protein previously found in Galleria mellonella. The B. mori sequence of the P150/sericin 6 ORF was previously incorrectly predicted and assigned to two smaller, uncharacterized genes. We present a new P150/sericin 6 gene model and show that it encodes a large protein of 467 kDa. It is characterized by repeats with a high proportion of threonine residues and a short conserved region with a cysteine knot motif (CXCXCX) at the C-terminus. Expression analyzes have shown that B. mori P150/ser6 has low transcriptional level in contrast to its G. mellonella homolog. We also discuss the synteny of homologous genes on corresponding chromosomes between moth species and possible phylogenetic relationships between P150/ser6 and cysteine knot mucins. Our results improve our understanding of the evolutionary relationships between adhesion proteins in different lepidopteran species.
Transcription levels of hes and its participation in Shiga Toxin-producing Escherichia coli biofilm formation isolated from cattle and foods of animal origin
Authors: Vélez María Victoria , Colello Rocío , Farias María Victoria Nieto , Paz Laura Stefania , Etcheverría Analía Ines , Vidal Roberto , Padola Nora Lía 
Published: November 2023

Abstract
Shiga toxin-producing Escherichia coli (STEC) are recognized as responsible for many cases of foodborne diseases worldwide. Cattle are the main reservoir of STEC, shedding the microorganisms in their feces. The serogroup STEC O91 has been associated with hemorrhagic colitis and hemolytic uremic syndrome. Molecular data on which STEC O91 causes human disease still needs to be included. LAA and its hes gene are related to the pathogenicity of STEC and the ability to form biofilms. Considering the frequent isolation of STEC O91, the biofilm-forming ability, and the possible role of hes in the pathogenicity of STEC, we propose to analyze the ability of STEC to form biofilms and to evaluate the expression of hes before and after of biofilm formation. All strains were classified as strong biofilm-forming. The hes expression showed variability between strains before and after biofilm formation, and this may be due to other genes carried by each strain. This study is the first to report the relationship between biofilm formation, and hes expression and proposes that the analysis and diagnosis of LAA, especially hes as STEC O91 virulence factors, could elucidate these unknown mechanisms There is no treatment for HUS, so it is necessary to know the survival and virulence mechanisms of STEC O91.
Application of spectral CT in D3 lymphadenectomy under a laparoscope for treating right-sided colorectal cancer
Authors: Zhang Ranhao , Shi Mengwei , Qiao Wenjuan , Mu Dongdong , Gao Yanrong , Zheng Liansheng 
Published: November 2023

Abstract
Objective: The positive aspects of using spectral CT in D3 lymphadenectomy under a laparoscope for the treatment of right-sided colorectal cancer (CRC) are discussed.
Methods: We enrolled right-sided CRC patients (n=40) and preoperative examinations by spectral CT revealed no distant metastasis. The pattern and orientation of the colon, tumor location, the positional correlation of lymph nodes and vessels, and the anatomy and alterations of key blood vessels were probed. Further, the relative spatial positions of different vessels were investigated using the 3D reconstruction technology of spectral CT. Finally, the results were compared with those observed during surgery.
Results: Examination using spectral CT, 3D reconstruction, and data analysis were performed for all 40 cases with successful completion of all the surgeries. Overall, the pattern and orientation of the colon, tumor location, the relation of lymph nodes and blood vessels, and the anatomy and variation of key blood vessels observed during surgery were identical to the 3D reconstructed images obtained by spectral CT before the surgery. This suggests that the accuracy of 3D reconstruction reached 100%.
Conclusion: The application of the 3D reconstruction technology of spectral CT before D3 lymphadenectomy under a laparoscope can help to visualize and determine the correlation between the location of a lesion and the anatomical location, hence assisting the surgery.
Complete sequence and genome characterization of Miscanthus virus M, a new betaflexivirus from Miscanthus sp.
Authors: Abrahamian Peter , Grinstead Samuel , Kinard Gary R , Goenaga Ricardo , Rott Philippe , Mollov Dimitre 
Published: November 2023

Abstract
A novel betaflexivirus, tentatively named Miscanthus virus M (MiVM), was isolated from Miscanthus sp. The complete genome of MiVM is 7,388 bp (excluding the poly-A tail). It is predicted to encode five open reading frames and has a genome organization similar to those of the families Alphaflexiviridae and Betaflexiviridae(Quinvirinae). The amino acid sequences of both the replicase and coat protein shared less than 45% identities with the corresponding sequences of members of either family. Phylogenetic analysis confirmed that MiVM belongs to the family Betaflexiviridaeand subfamily Quiniviriane but this virus did not cluster with any currently recognized genus sufficiently to be placed in it. We propose that Miscanthus virus M represents a novel species and genus within the family Betaflexiviridae.
Value of bronchial amylase level for predicting ventilator associated pneumonia in intubated adults: a systematic review and meta-analysis
Authors: Xu Tingzhen , Shen Qinkang , He Yuting , Pan Xiaozhuang , Huang Haijun , Xu Hua 
Published: November 2023

Abstract
Background: The ability of bronchial amylase level for predicting ventilator associated pneumonia (VAP) has been extensively studied with conflicting results. This meta-analysis aimed to explore the value of bronchial amylase for predicting VAP in intubated adults.
Methods: PubMed, Embase, and Cochrane Central Register of Controlled Trials were searched up to November 2023. The diagnostic odds ratio (DOR), sensitivity, and specificity were calculated. The summary receiver operating characteristic curve was estimated, and the area under the curve (AUROC) was calculated.
Results: Overall, six studies including 769 patients were included in this review, of whom 273 (36%) were developed VAP. The cutoff values of bronchial amylase level were ranged from 8.1 U/L to 4681.5U/L. Heterogeneity between studies was assessed with an overall Q = 1.99, I2 = 0, and P = 0.185, The pooled sensitivity and specificity for the overall population were 0.78 [95% confidence interval (CI) 0.67–0.86] and 0.75(95% CI 0.56–0.88) respectively. The DOR was 11(95% CI 3.0–40.0). The pooled AUROC was 0.83 (95%CI 0.80 - 0.86).
Conclusions: The bronchial amylase is a helpful marker for predicting VAP in intubated adults. However, it cannot be recommended as the single definitive test for VAP, but rather it must be interpreted in context with information from careful medical history, physical examination, and when feasible, microbiological assessment.