bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergid cell calcium oscillations refine understanding of FERONIA/LORELEI signaling during interspecific hybridization
Authors: Ponvert Nathaniel , Johnson Mark A. 
Published: November 2023

Abstract
Key message
Pollen tubes from closely related species and mutants lacking pollen tube MYB transcription factors are able to initiate FER/LRE-dependent synergid cell calcium oscillations.

Abstract
Reproductive isolation leads to the evolution of new species; however, the molecular mechanisms that maintain reproductive barriers between sympatric species are not well defined. In flowering plants, sperm cells are immotile and are delivered to female gametes by the pollen grain. After landing on the stigmatic surface, the pollen grain germinates a polarized extension, the pollen tube, into floral tissue. After growing via polar extension to the female gametes and shuttling its cargo of sperm cells through its cytoplasm, the pollen tube signals its arrival and identity to synergid cells that flank the egg. If signaling is successful, the pollen tube and receptive synergid cell burst, and sperm cells are released for fusion with female gametes. To better understand cell–cell recognition during reproduction and how reproductive barriers are maintained between closely related species, pollen tube-initiated synergid cell calcium ion dynamics were examined during interspecific crosses. It was observed that interspecific pollen tubes successfully trigger synergid cell calcium oscillations—a hallmark of reproductive success—but signaling fails downstream of key signaling genes and sperm are not released. This work further defines pollen tube–synergid cell signaling as a critical block to interspecific hybridization and suggests that the FERONIA/LORELEI signaling mechanism plays multiple parallel roles during pollen tube reception.
“Take the tablet or don’t take the tablet?”—A qualitative study of patients’ experiences of self-administering anti-cancer medications related to adherence and managing side effects
Authors: Dang Thu Ha , O’Callaghan Clare , Alexander Marliese , Burbury Kate , Jayaraman Prem Prakash , Wickramasinghe Nilmini , Schofield Penelope 
Published: November 2023

Abstract
Purpose
Medication non-adherence is a well-recognised problem in cancer care, negatively impacting health outcomes and healthcare resources. Patient-related factors influencing medication adherence (MA) are complicated and interrelated. There is a need for qualitative research to better understand their underlying interaction processes and patients’ needs to facilitate the development of effective patient-tailored complex interventions. This study aimed to explore experiences, perceptions, and needs relating to MA and side effect management of patients who are self-administering anti-cancer treatment.

Methods
Semi-structured audio-recorded interviews with patients who have haematological cancer were conducted. A comparative, iterative, and predominantly inductive thematic analysis approach was employed.

Results
Twenty-five patients from a specialist cancer hospital were interviewed. While self-administering cancer medications at home, patients’ motivation to adhere was affected by cancer-related physical reactions, fears, cancer literacy and beliefs, and healthcare professional (HCP) and informal support. Patients desired need for regular follow-ups from respectful, encouraging, informative, responsive, and consistent HCPs as part of routine care. Motivated patients can develop high adherence and side effect self-management over time, especially when being supported by HCPs and informal networks.

Conclusion
Patients with cancer need varied support to medically adhere to and manage side effects at home. HCPs should adapt their practices to meet the patients’ expectations to further support them during treatment. We propose a multi-dimensional and technology- and theory-based intervention, which incorporates regular HCP consultations providing tailored education and support to facilitate and maintain patient MA and side effect self-management.
Lesson learned in endoscopic endonasal dens resection for C1–C2 spinal cord decompression
Authors: Portonero Irene , Lo Bue Enrico , Penner Federica , Di Perna Giuseppe , Baldassarre Bianca Maria , De Marco Raffaele , Pesaresi Alessandro , Garbossa Diego , Pecorari Giancarlo , Zenga Francesco 
Published: November 2023

Abstract
Purpose
Endoscopic endonasal approach (EEA) is the safest and most effective technique for odontoidectomy. Nevertheless, this kind of approach is yet not largely widespread. The aim of this study is to share with the scientific community some tips and tricks with our ten-year-old learned experience in endoscopic endonasal odontoidectomy (EEO), which remains a challenging surgical approach.

Material and methods
Our case series consists of twenty-one (10 males, 11 females; age range of 34–84 years) retrospectively analyzed patients with ventral spinal cord compression for non-reducible CVJ malformation, treated with EEA from July 2011 to March 2019.

Results
The results have recently been reported in a previous paper. The only intraoperative complication observed was intraoperative cerebrospinal fluid (CSF) leak (9.5%), without any sign of post-operative CSF leak.

Conclusions
Considering our experience, EEO represents a valid and safe technique to decompress neural cervical structures. Despite its technical complexity, mainly due to the use of endoscope and the challenging surgical area, with this study we encourage the use of EEO displaying our experience-based surgical tips and tricks.
The effect of hard tissue defects on the clinical outcome of endodontic microsurgery: a systematic review and meta-analysis
Authors: Sabeti Mohammad , Ihsan Mohammad Saqib , Kharat Piyusha , Azarpazhooh Amir 
Published: November 2023

Abstract
Objectives
The purpose of this systematic review was to appraise the existing literature on the effect of hard tissue defects on the clinical outcome of endodontic microsurgery (EMS).

Methods
MEDLINE (PubMed), Embase, Web of Science, Cochrane Library and grey literature were searched from January 2000 to May 2023. Study selection and data extraction were performed in duplicate. Eligible studies were critically appraised for the risk of bias using the Cochrane Risk of bias tool. The quality of evidence was assessed using GRADE. Review Manager (RevMan Computer program Version 5.4, The Cochrane Collaboration, 2020) was utilized and the Mantel Haenszel fixed or random effects model was applied, depending on the heterogeneity of the studies. Meta-analysis was performed to estimate the Risk ratio (RR) and 95% Confidence Interval (CIs) to correlate the effects of these factors on treatment outcomes.

Results
Nineteen studies were included. The EMS overall pooled success rate was 84.5%. Five characteristics of hard tissue were identified. The size of the lesion (Small ≤ 5 mm: 78.4% vs. Large > 5 mm: 63.3%, RR = 1.12, 95% CI 1.00–1.26, P ≤ .05), significantly affected the outcomes of EMS. Endodontic lesions exhibited slightly better outcomes than endodontic-periodontal lesions (81.4% vs. 68.2%, RR = 1.14 95% CI 0.98–1.33, P > .05). Cases with the height of the buccal bone > 3 mm also exhibited slightly better outcomes (91.5% vs. 71.4%, RR = 1.20, 95% CI 0.88–1.62, P > .05). Additionally, through and through lesions exhibited better outcomes when grafting was completed during the EMS procedure both in 2D (RR = 1.12 95% CI 0.97–1.29, P > .05) and 3D evaluation ((RR = 1.28 95% CI 0.69–2.37 P > .05). The overall quality of evidence was graded as low to high.

Conclusion
With a low to high quality of evidence, the size of the lesion is a key prognostic variable that significantly affects the outcome of EMS, as lesions ≤ 5 mm exhibit better outcomes as compared to larger lesions.

Clinical significance
The presence of hard tissue defects can affect the outcome of endodontic microsurgery (EMS). The presented data can aid the clinicians’ decision-making process by examining certain pre-operative prognostic variables, when considering EMS as a treatment option. Clinical cases with more favorable hard tissue characteristics lead to a better prognosis in EMS.
Gender-related stress factors and emotional perception in migraine: a structured online questionnaire in migraine patients and controls
Authors: Delussi Marianna , Piraino Giulia , Guerzoni Simona , Castro Flavia Lo , Sances Grazia , Guaschino Elena , Vaghi Gloria , Grazzi Licia , Sacco Simona , Onofri Agnese , Paparella Giulia , Prudenzano Maria Pia , Roca Maria Elena , Fallacara Adriana , Cevoli Sabina , Pierangeli Giulia , Sarchielli Paola , Bellotti Alessia , Invitto Sara , de Tommaso Marina 
Published: November 2023

Abstract
Background
While migraine is markedly prevalent in women, gender-related phenotype differences were rarely assessed. For this reason, we investigated, through a multicenter observational cross-sectional study, based on an online questionnaire, gender-related differences in stress factors, emotions, and pain perception in migraine patients and controls and their impact on migraine severity.

Methods
The study was designed as an online questionnaire. The link was emailed to healthy subjects (C) and migraine patients (MIG) (age 18–75, education ≥ 13 years) recruited during the first visit in 8 Italian Headache Centers adhering to Italian Society for Headache Study (SISC). The questionnaire included personal/social/work information, the Perceived Stress Scale, the Romance Quality Scale, the Emotion Regulation Questionnaire, the Beck Anxiety Inventory, the Body Perception Questionnaire, the pain perception, and a self-assessment of migraine severity in the last 3 months.

Results
202 MIG and 202 C completed the survey. Independently from gender, migraine was characterized by higher pain sensitivity and more severe partner relationships. The female gender, in MIG, exhibited higher anxiety scores, body awareness, and reduced emotional suppression. Body awareness and emotional suppression were discriminating factors between genders in control and migraine groups without relevant influence on disease features. Perceived perception of migraine severity was similar between genders.

Conclusion
Gender-related emotional and stress factors did not contribute to delineate a distinct phenotype in migraine men and women. The possible impact of emotional and stress factors characterizing genders could be considered for a single case–tailored therapeutic approach.
The effect of moderate broadband noise on cognitive performance: a systematic review
Authors: Zhou Hang , Molesworth Brett R. C. , Burgess Marion , Hatfield Julie 
Published: November 2023

AbstractIndividuals in occupational settings are often exposed to moderate levels (e.g., < 85 dBA) of background noise (i.e., broadband noise) while performing cognitive-based tasks. Exposure to such noise may affect individuals’ cognitive performance and thus affect desired performance level (e.g., safety performance). The present review outlines the effect of moderate broadband noise on indices of cognition such as reaction time, attention, short-term and long-term memory, and high(er)-order cognitive tasks such as decision-making, mental arithmetic, grammatic reasoning, or reading comprehension. The present review highlights limitations associated with moderators, and their effect on broadband noise. 43 studies ranging from 1980 to February 2023 were selected and analysed to investigate this issue. Reviewed results offered minimal evidence for a negative effect of moderate broadband noise on cognitive performance. Negative effects that were found, were often evident only under specific circumstances. The roles of cognitive load, repeated session, and individual differences (e.g., sex) were found to differ from theoretical predictions based on evidence from broader noise(s), which highlights the distinctive characteristics of the mild stressor; hence an area for future research.
Insight in molecular degradation patterns and co-metabolism during rose waste co-composting
Authors: de Nijs E. A. , Jansen B. , Absalah S. , Bol R. , Tietema A. 
Published: November 2023

AbstractComposting is recognized as a sustainable waste management strategy. However, little is known about green waste, and specifically rose waste, degradation patterns during composting. This study aimed (1) to gain insight in the underlying decomposition patterns during rose waste composting and (2) to identify co-metabolisms of ligneous material. Five different compost mixtures were tested ranging from pure rose waste to mixtures with tomato waste, kalanchoe waste or mature compost added. Samples were taken during a six-month experiment and analyzed by pyrolysis-GC/MS. The temporal trends in the relative abundance of 10 different compound groups were measured. Lignin and aliphatic compounds together accounted for ≥ 50% of the quantified pyrolysis products, but with changing contributions during composting. The relative abundance of polysaccharides and terpenes strongly decreased with more than 60% in the first 2 months. The simultaneous decrease in relative abundance of lignin and polysaccharides during initial composting phase indicated co-metabolism of lignin. The results from this study showed that while the presence of lignin is commonly regarded as a challenge in composting, it actually undergoes degradation through distinct mechanisms at the various composting stages.
Neuromodulation Through Magnetic Fields Irradiation with AT-04 Improves Hyperalgesia in a Rat Model of Neuropathic Pain via Descending Pain Modulatory Systems and Opioid Analgesia
Authors: Kohno Tatsuro , Takaki Kaori , Kishita Kaori , Mitsutake Kazunori , Tofuku Nozomu , Kishita Iwao 
Published: November 2023

AbstractNeuromodulation through magnetic fields irradiation with ait® (AT-04), a device that irradiates a mixed alternating magnetic fields (2 kHz and 83.3 MHz), has been shown to have high efficacy for fibromyalgia and low back pain in our previous clinical trials. The aim of this study was to elucidate the underlying analgesic mechanism of the AT-04 using the partial sciatic nerve ligation (PSL) model as an animal model of neuropathic pain. AT-04 was applied to PSL model rats with hyperalgesia and its pain-improving effect was verified by examining mechanical allodynia using the von Frey method. The results demonstrated a significant improvement in hyperalgesia in PSL model rats. We also examined the involvement of descending pain modulatory systems in the analgesic effects of AT-04 using antagonism by serotonin and noradrenergic receptor antagonists. These antagonists significantly reduced the analgesic effect of AT-04 on pain in PSL model rats by approximately 50%. We also measured the amount of serotonin and noradrenaline in the spinal fluid of PSL model rats using microdialysis during AT-04 treatment. Both monoamines were significantly increased by magnetic fields irradiation with AT-04. Furthermore, we evaluated the involvement of opioid analgesia in the analgesic effects of AT-04 using naloxone, the main antagonist of the opioid receptor, and found that it significantly antagonized the effects by approximately 60%. Therefore, the analgesic effects of AT-04 in PSL model rats involve both the endogenous pain modulation systems, including the descending pain modulatory system and the opioid analgesic system.
Graphical Abstract
Growth response of the picoplanktic Picocystis salinarum and the microplanktic Limnospira (Arthrospira) fusiformis strains from Lake Nakuru (Kenya) to rapidly changing environmental conditions
Authors: Pálmai Tamás , Szabó Beáta , Lengyel Edina , Kotut Kiplagat , Krienitz Lothar , Padisák Judit 
Published: November 2023

AbstractThe East African soda lakes are known worldwide for their huge populations of lesser flamingos. Their phytoplankton community is often dominated by the cyanobacterium Limnospira fusiformis, the main food of lesser flamingos. In the early 2010s, the population of the cyanobacterium collapsed and the picoplanktic green alga Picocystis salinarum became dominant in Lake Nakuru. Consequently, lesser flamingos had to migrate to other lakes in search of food. To establish the reasons for the success of P. salinarum, photosynthesis measurements have been performed on monoalgal cultures of both species. The examined environmental variables (temperature, light intensity) were not responsible for the dominance of P. salinarum either alone or in their any combination. Moreover, photosynthetic activity of the cyanobacterium was higher by an order of magnitude during all light and temperature treatments. Co-cultivation of L. fusiformis and P. salinarum in a chemostat revealed that a possible reason for the Limnospira replacement can be a rapid and remarkable increase of conductivity, as P. salinarum showed higher level of tolerance to this rapid change. Shortly after returning to the initial conductivity levels, the population of L. fusiformis recovered quickly.
Association Between Social Isolation and Mental Well-Being in Later Life. What is the Role of Loneliness?
Authors: Vasile Marian , Aartsen Marja , Precupetu Iuliana , Tufă Laura , Dumitrescu Diana-Alexandra , Radogna Rosa Maria 
Published: November 2023

AbstractIn this study, we evaluate whether the association between three types of social isolation (SI) and mental well-being (MWB) is mediated by loneliness. We include a pooled sample (N = 8,525) of people aged 65 or older, participating in the 2016 European Quality of Life Survey (EQLS). MWB is assessed with the World Health Organization Well-Being Index (WHO-5), and SI is operationalised as living alone, having no or little contact with family members, or having no or little contact with friends and neighbours. We assess a full mediation model with loneliness as a mediator between each type of SI and MWB, which we compare with a partial mediation model and control for age, gender, urbanity, and subjective income. Estimates for the full mediation model indicate that each type of SI is associated with enhanced loneliness, which in turn is negatively associated with MWB. In addition, the total effect of each type of SI on MWB is negative, with the strongest negative path from living alone to MWB. However, when including the direct effects of each type of SI on MWB in the partial mediating model, the total effect of contact with family members on MWB loses statistical significance. The specific indirect effects of the three facets of SI on MWB remain significant and negative. These findings suggest that different aspects of SI have distinct relationships with MWB, and that absence of contact with family members influences MWB only if they activate the feeling of loneliness.
Minimal data requirement for realistic endoscopic image generation with Stable Diffusion
Authors: Kaleta Joanna , Dall’Alba Diego , Płotka Szymon , Korzeniowski Przemysław 
Published: November 2023

Abstract
Purpose
Computer-assisted surgical systems provide support information to the surgeon, which can improve the execution and overall outcome of the procedure. These systems are based on deep learning models that are trained on complex and challenging-to-annotate data. Generating synthetic data can overcome these limitations, but it is necessary to reduce the domain gap between real and synthetic data.

Methods
We propose a method for image-to-image translation based on a Stable Diffusion model, which generates realistic images starting from synthetic data. Compared to previous works, the proposed method is better suited for clinical application as it requires a much smaller amount of input data and allows finer control over the generation of details by introducing different variants of supporting control networks.

Results
The proposed method is applied in the context of laparoscopic cholecystectomy, using synthetic and real data from public datasets. It achieves a mean Intersection over Union of 69.76%, significantly improving the baseline results (69.76 vs. 42.21%).

Conclusions
The proposed method for translating synthetic images into images with realistic characteristics will enable the training of deep learning methods that can generalize optimally to real-world contexts, thereby improving computer-assisted intervention guidance systems.
A comparative study of robot-assisted thoracoscopic surgery and conventional approaches for short-term outcomes of anatomical segmentectomy
Authors: Haruki Tomohiro , Kubouchi Yasuaki , Kidokoro Yoshiteru , Matsui Shinji , Ohno Takashi , Kojima Shunsuke , Nakamura Hiroshige 
Published: November 2023

Abstract
Objectives
Since anatomical segmentectomy requires meticulous dissection of the segmental pulmonary vessels and bronchus, robot-assisted thoracoscopic surgery (RATS) has been widely adopted in recent years. We investigated the usefulness of RATS segmentectomy by comparing perioperative outcomes with conventional approaches including open thoracotomy or video-assisted thoracoscopic surgery (VATS). We compared perioperative outcomes of segmentectomy between RATS and conventional approaches including open thoracotomy or video-assisted thoracoscopic surgery (VATS).

Methods
This single-institutional retrospective study comprised 231 patients with primary lung cancer who underwent segmentectomy by RATS or conventional approaches between January 2011 and December 2022. Surgical outcomes and postoperative complications were analyzed among patients whose background factors were adjusted by propensity score matching (PSM).

Results
Before PSM, there were significant differences in age, smoking status, and types of segmentectomy. After PSM, 126 patients (63 patients in each group) were included in this analysis. The RATS group had significantly shorter operative time (154 vs 210 min; p < 0.01), fewer bleeding amounts (10 vs 40 mL; p < 0.01), and shorter duration of chest drainage (2 vs 2 days; p = 0.04) than the conventional-approach group. There was no significant difference in the incidence of all complications; however, the incidence of postoperative pneumonia was significantly lower than in the conventional-approach group (p = 0.02).

Conclusions
RATS segmentectomy is proposed to be useful. It was suggested that RATS segmentectomy may be useful with better perioperative results than the conventional approach. Further studies on oncological long-term outcomes and cost–benefit comparisons are needed.
Nachhaltigkeit und ihre Bedeutung für die Soziale Arbeit
Authors: Schmidt Marcel 
Published: November 2023

ZusammenfassungVor dem Hintergrund der Notwendigkeit klimakrisenbedingter gesellschaftlicher Veränderungen und der immer offensichtlicher werdenden Tatsache, dass diese Veränderungen eine grundlegende Umgestaltung des Sozialen bedeuten und damit Soziale Arbeit in ihrem disziplinären wie professionellen Kern adressiert ist, ist in der Sozialen Arbeit in den letzten zwei bis drei Jahren eine Diskussion um ökologische, soziale und ökonomische Nachhaltigkeit entstanden. Der Artikel fasst diese Diskussion zusammen und stellt bislang noch offene Fragen und inhaltliche Lücken heraus. Als Ergebnis lässt sich festhalten, dass Soziale Arbeit angesichts der drohenden Krisen und anstehenden Veränderungsnotwendigkeiten auf subjektiver wie gesellschaftlicher Ebene mehr denn je gefordert ist, sich wieder als professionelle Unterstützung zivilgesellschaftlicher Politikgestaltung zu verstehen und entsprechend zu agieren.