bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics analysis reveals the molecular regulatory network underlying the prevention of Lactiplantibacillus plantarum against LPS-induced salpingitis in laying hens
Authors: Song Dan , Li Aike , Chen Bingxu , Feng Jia , Duan Tao , Cheng Junlin , Chen Lixian , Wang Weiwei , Min Yuna 
Published: November 2023

Abstract
Background
Salpingitis is one of the common diseases in laying hen production, which greatly decreases the economic outcome of laying hen farming. Lactiplantibacillus plantarum was effective in preventing local or systemic inflammation, however rare studies were reported on its prevention against salpingitis. This study aimed to investigate the preventive molecular regulatory network of microencapsulated Lactiplantibacillus plantarum (MLP) against salpingitis through multi-omics analysis, including microbiome, transcriptome and metabolome analyses.

Results
The results revealed that supplementation of MLP in diet significantly alleviated the inflammation and atrophy of uterus caused by lipopolysaccharide (LPS) in hens (P < 0.05). The concentrations of plasma IL-2 and IL-10 in hens of MLP-LPS group were higher than those in hens of LPS-stimulation group (CN-LPS group) (P < 0.05). The expression levels of TLR2, MYD88, NF-κB, COX2, and TNF-α were significantly decreased in the hens fed diet supplemented with MLP and suffered with LPS stimulation (MLP-LPS group) compared with those in the hens of CN-LPS group (P < 0.05). Differentially expressed genes (DEGs) induced by  MLP were involved in inflammation, reproduction, and calcium ion transport. At the genus level, the MLP supplementation significantly increased  the abundance of Phascolarctobacterium, whereas decreased the abundance of Candidatus_Saccharimonas in LPS challenged hens (P < 0.05). The metabolites altered by dietary supplementation with MLP were mainly involved in galactose, uronic acid, histidine, pyruvate and primary bile acid metabolism. Dietary supplementation with MLP inversely regulates LPS-induced differential metabolites such as LysoPA (24:0/0:0) (P < 0.05).

Conclusions
In summary, dietary supplementation with microencapsulated Lactiplantibacillus plantarum prevented salpingitis by modulating the abundances of Candidatus_Saccharimonas, Phascolarctobacterium, Ruminococcus_torques_group and Eubacterium_hallii_group while downregulating the levels of plasma metabolites, p-tolyl sulfate, o-cresol and N-acetylhistamine and upregulating S-lactoylglutathione, simultaneously increasing the expressions of CPNE4, CNTN3 and ACAN genes in the uterus, and ultimately inhibiting oviducal inflammation.
A validation study of the Arabic version of the Warwick-Edinburgh Mental Well-being scale among undergraduate students
Authors: Zayed Kashef , Omara Ehab , Al-Shamli Ali , Al-Rawahi Nasser , Haramlah Ahmed Al , Al-Attiyah Asma A. , Al-Haddabi Badriya , Al-Yarobi Ali , Al-Busafi Majid , Al-Jadidi Khalifa 
Published: November 2023

Abstract
Background
The main aim of this study was to assess the validity of the Warwick-Edinburgh Mental Well-being Scale (WEMWBS) and the short version of the Warwick-Edinburgh Mental Well-being Scale (SWEMWBS) and to evaluate the metric properties of both versions by using a sample of undergraduate students from three Gulf Cooperation Council (GCC) countries (Oman, Qatar, and Saudi Arabia).

Methods
Six hundred ninety undergraduate students (340 M and 350 F; mean age = 21.16 ± 2.44) from Oman, Qatar, and Saudi Arabia (NOM = 238, NQA = 215, NSA = 237), voluntarily participated in this cross-section study. All of them responded to the WEMWBS, Basic Psychological Needs Satisfaction Frustration (BPNSFS), and Beck Depression Inventory (BDI-II). The methodology involved utilizing descriptive statistics to understand the data’s characteristics, internal consistency analysis for reliability, correlation analysis for convergent validity, confirmatory factor analysis to validate the scales, and measurement invariance testing to ensure cross-group comparability. Model fit indices were employed to gauge the goodness of fit.

Results
The translated Arabic versions of the WEMWBS and SWEMWBS showed good reliability, with Cronbach’s alpha values of 0.867 and 0.772, respectively. The findings of confirmatory factor analysis asserted the one-factor solution to interpret the item variances of the 14-item WEMWBS and 7-item SWEMWBS. The WEMWBS and SWEMWBS also showed significant positive relationships with need satisfaction and negative relationship with need frustration, and depression. Moreover, the SWEMWBS showed partial scalar invariance across genders and countries, while the WEMWBS showed only partial metric invariance across the three countries and partial scalar invariance across genders.

Conclusions
Our study highlights the appropriateness of both versions of the Warwick-Edinburgh Mental Well-being Scale (WEMWBS) in assessing the psychological well-being of Arab undergraduate students. The employment of these tools is strongly encouraged for the assessment of mental well-being within a comparable adult population.
Using audience segmentation to identify implementation strategies to improve PrEP uptake among at-risk cisgender women: a mixed-methods study protocol
Authors: Pellowski Jennifer A. , Price Devon M. , Desir Arielle , Golub Sarit , Operario Don , Purtle Jonathan 
Published: November 2023

Abstract
Background
In the USA, 19% of new HIV infections occur among cisgender women (cis women); however, only 10% of eligible cis women have been prescribed pre-exposure prophylaxis (PrEP) for the prevention of HIV infection (an evidence-based intervention). A fundamental challenge for expanding HIV prevention to cis women is ensuring implementation strategies are tailored to the various healthcare settings in which cis women seek care and the heterogeneous providers nested within these settings. This project’s specific aims are to (1) explore clinician-level characteristics and organizational climate factors that are related to variability in adoption of PrEP service delivery as an evidence-based intervention for cis women; (2) identify latent audience segments of women’s health providers as the related to PrEP acceptability, adoption, and maintenance and analyze demographic correlates of these segments; and (3) identify audience segment-specific implementation strategies to facilitate the adoption of PrEP as an evidence-based intervention among at-risk cis women.

Methods
Using the i-PARIHS framework, this mixed-methods study examines three domains for guiding audience segmentation to facilitate PrEP implementation for cis women: innovation (degree of fit with existing practices, usability), recipient beliefs and knowledge and context factors (organizational culture, readiness for change), needs to determine appropriate facilitation methods. To achieve aim 1, qualitative interviews will be conducted with PrEP-eligible cis women, women’s health providers, and other key stakeholders. Aim 2 will consist of a quantitative survey among 340 women’s health providers. Latent class analysis will be used to facilitate audience segmentation.
To achieve aim 3, a panel of 5–8 providers for each audience segment will meet and engage in iterative discussions guided by Fernandez’s implementation mapping to identify (1) implementation outcomes and performance objectives, determinants, and change objectives and (2) determine and refine of implementation strategies for each audience segment.

Discussion
This exploratory mixed methods study will provide an empirical foundation to inform the development implementations strategies aimed at increasing PrEP delivery to cis women among heterogenous groups of providers.
A structural equation model of CFIR inner and outer setting constructs, organization characteristics, and national DPP enrollment
Authors: Madrigal Lillian , Haardörfer Regine , Kegler Michelle C. , Piper Sarah , Blais Linelle M. , Weber Mary Beth , Escoffery Cam 
Published: November 2023

Abstract
Background
The National Diabetes Prevention Program (DPP) has made great strides in increasing accessibility to its year-long, evidence-based lifestyle change program, with around 3000 organizations having delivered the program. This large dissemination effort offers a unique opportunity to identify organization-level factors associated with program implementation and reach (enrollment) across diverse settings. The purpose of this study was to quantitatively examine the relationships among Consolidated Framework for Implementation Research (CFIR) Inner Setting and Outer Setting constructs and the implementation outcome of reach.

Methods
This study analyzed data from a 2021 cross-sectional online survey with 586 National DPP Staff (lifestyle coaches, master trainers, program coordinators) with information about their organization, implementation outcomes, and responses to quantitative CFIR Inner Setting and Outer Setting construct items. Structural equation modeling was used to test a hypothesized path model with Inner and Outer Setting variables to explore direct and indirect pathways to enrollment.

Results
The CFIR items had good internal consistency and indicated areas of implementation strength and weakness. Eight variables included as part of the CFIR structural characteristics and one organization characteristic variable had significant direct relationships with enrollment. The length of delivery, number of lifestyle coaches, number of full-time staff, large organization size, and organizations delivering in rural, suburban, and/or urban settings all had positive significant direct relationships with enrollment, while academic organizations and organizations with only non-White participants enrolled in their National DPP lifestyle change programs had a negative association with enrollment.

Conclusions
Participant reach is an important implementation outcome for the National DPP and vital to making population-level decreases in diabetes incidence in the USA. Our findings suggest that to facilitate enrollment, program implementers should focus on organizational structural characteristics such as staffing. Strengths of this study include the use of adapted and newly developed quantitative CFIR measures and structural equation modeling. Health prevention programs can use the methods and findings from this study to further understand and inform the impact of organization factors on implementation outcomes.
Robot-assisted right wedge-shaped sleeve lower bilobectomy with knotless suture anastomosis: a case report
Authors: Oyamatsu Hironori , Shimura Yusuke , Kiriyama Ryota , Okagawa Takehiko , Fujimura Takaki , Niimi Seijirou 
Published: November 2023

Abstract
Background
In bronchoplasty of wedge resections, it is necessary to transect the bronchus at a sharp angle and depth. As a result, anastomoses after wedge resections have the disadvantages of poor visibility and operability. Here, we report a case of right wedge-shaped sleeve bilobectomy that was successfully performed with continuous knotless suturing using robotic assistance.

Case presentation
An 81-year-old male patient was referred for the treatment of a tumor in the right lower lobe, which protruded into the bronchus intermedius. The tumor was diagnosed as squamous cell carcinoma by transbronchial biopsy, cT1cN1M0 stage IIB carcinoma for which surgery was indicated. Because the pulmonary middle lobe artery was involved and a resection margin from the tumor protruding into the bronchial mucosal epithelium was necessary, a right wedge-shaped sleeve bilobectomy was performed. The bronchial anastomosis was performed with robotic assistance. After dissection of pulmonary vessels and interlobes, the upper lobe bronchial bifurcation was transected in a wedged shape, and a lower bilobectomy was performed. The bronchi were sutured continuously with knotless sutures. A continuous suture was performed from the ventral to the caudal side. After suturing to the dorsal side, another continuous suturing was performed from the cranial side. Continuous sutures were made until each thread passed through the other. Pericardial fat was wrapped around the anastomosis.

Conclusions
A better visual field could be obtained owing to robot-assisted surgery, and robotic arms enabled an accurate and safe operation. Furthermore, continuous suturing using a knotless suture made it easier for the sutures to be handled and enabled bronchial anastomosis without assistance.
SKYSCRAPER-02: Tiragolumab in Combination With Atezolizumab Plus Chemotherapy in Untreated Extensive-Stage Small-Cell Lung Cancer
Authors: Rudin Charles M. , Liu Stephen V. , Soo Ross A. , Lu Shun , Hong Min Hee , Lee Jong-Seok , Bryl Maciej , Dumoulin Daphne W. , Rittmeyer Achim , Chiu Chao-Hua , Ozyilkan Ozgur , Johnson Melissa , Navarro Alejandro , Novello Silvia , Ozawa Yuichi , Tam Sammi Hiu , Patil Namrata S. , Wen Xiaohui , Huang Meilin , Hoang Tien , Meng Raymond , Reck Martin 
Published: November 2023

PURPOSE The phase III SKYSCRAPER-02 study determined whether the benefits of atezolizumab plus carboplatin and etoposide (CE) could be enhanced by the addition of tiragolumab in untreated extensive-stage small-cell lung cancer (ES-SCLC). We report final progression-free survival (PFS) and overall survival (OS) analyses. METHODS Patients received tiragolumab 600 mg/placebo, plus atezolizumab 1,200 mg and CE (four cycles), then maintenance tiragolumab/placebo plus atezolizumab. Primary end points were investigator-assessed PFS and OS in patients without history/presence of brain metastases (primary analysis set [PAS]). Additional end points included PFS and OS in all patients regardless of brain metastases status (full analysis set [FAS]), response, and safety. RESULTS Four hundred ninety patients were randomly assigned (FAS): 243 to tiragolumab arm and 247 to control arm. At the cutoff date (February 6, 2022; median duration of follow-up, 14.3 months [PAS] and 13.9 months [FAS]), final analysis of PFS in the PAS (n = 397) did not reach statistical significance (stratified hazard ratio [HR], 1.11; P = .3504; median, 5.4 months tiragolumab v 5.6 months control). At the cutoff date (September 6, 2022; median duration of follow-up, 21.2 months [FAS]), median OS in the PAS at final OS analysis was 13.1 months in both arms (stratified HR, 1.14; P = .2859). Median PFS and OS in the FAS were consistent with the PAS. The proportion of patients with immune-mediated adverse events (AEs) in the tiragolumab and control arms was 54.4% and 49.2%, respectively (grade 3/4: 7.9% and 7.7%). AEs leading to treatment withdrawal occurred in 8.4% and 9.3% of tiragolumab- and control-treated patients, respectively. CONCLUSION Tiragolumab did not provide additional benefit over atezolizumab and CE in untreated ES-SCLC. The combination was well tolerated with no new safety signals.
Dynamic Nomogram for Predicting the Risk of Perioperative Neurocognitive Disorders in Adults
Authors: Song Ai-lin , Li Yu-jie , Liang Hao , Sun Yi-zhu , Shu Xin , Huang Jia-hao , Yang Zhi-yong , He Wen-quan , Zhao Lei , Zhu Tao , Zhong Kun-hua , Chen Yu-wen , Lu Kai-zhi , Yi Bin 
Published: November 2023

BACKGROUND:
Simple and rapid tools for screening high-risk patients for perioperative neurocognitive disorders (PNDs) are urgently needed to improve patient outcomes. We developed an online tool with machine-learning algorithms using routine variables based on multicenter data.

METHODS:
The entire dataset was composed of 49,768 surgical patients from 3 representative academic hospitals in China. Surgical patients older than 45 years, those undergoing general anesthesia, and those without a history of PND were enrolled. When the patient’s discharge diagnosis was PND, the patient was in the PND group. Patients in the non-PND group were randomly extracted from the big data platform according to the surgical type, age, and source of data in the PND group with a ratio of 3:1. After data preprocessing and feature selection, general linear model (GLM), artificial neural network (ANN), and naive Bayes (NB) were used for model development and evaluation. Model performance was evaluated by the area under the receiver operating characteristic curve (ROCAUC), the area under the precision-recall curve (PRAUC), the Brier score, the index of prediction accuracy (IPA), sensitivity, specificity, etc. The model was also externally validated on the multiparameter intelligent monitoring in intensive care (MIMIC) Ⅳ database. Afterward, we developed an online visualization tool to preoperatively predict patients’ risk of developing PND based on the models with the best performance.

RESULTS:
A total of 1051 patients (242 PND and 809 non-PND) and 2884 patients (6.2% patients with PND) were analyzed on multicenter data (model development, test [internal validation], external validation-1) and MIMIC Ⅳ dataset (external validation-2). The model performance based on GLM was much better than that based on ANN and NB. The best-performing GLM model on validation-1 dataset achieved ROCAUC (0.874; 95% confidence interval [CI], 0.833–0.915), PRAUC (0.685; 95% CI, 0.584–0.786), sensitivity (72.6%; 95% CI, 61.4%–81.5%), specificity (84.4%; 95% CI, 79.3%–88.4%), Brier score (0.131), and IPA (44.7%), and of which the ROCAUC (0.761, 95% CI, 0.712–0.809), the PRAUC (0.475, 95% CI, 0.370–0.581), Brier score (0.053), and IPA (76.8%) on validation-2 dataset. Afterward, we developed an online tool (https://pnd-predictive-model-dynnom.shinyapps.io/ DynNomapp/) with 10 routine variables for preoperatively screening high-risk patients.

CONCLUSIONS:
We developed a simple and rapid online tool to preoperatively screen patients’ risk of PND using GLM based on multicenter data, which may help medical staff’s decision-making regarding perioperative management strategies to improve patient outcomes.
The Role of Stereotactic Radiosurgery in Patients With Brain Metastases From Colorectal Cancers
Authors: Taori Suchet , Wei Zhishuo , Deng Hansen , Lunsford L. Dade , Niranjan Ajay 
Published: November 2023

BACKGROUND AND OBJECTIVES:
The role of stereotactic radiosurgery (SRS) in patients with brain metastases (BMs) from colorectal cancers (CRCs) has not been established. The authors present a single-institution experience of patients with CRC who underwent SRS with metastatic brain spread.

METHODS:
We retrospectively analyzed 111 patients with metastatic CRC (64 female, 57.7%), with 449 BMs treated with Gamma Knife SRS between 2000 and 2022. The median age during SRS was 63 years (range: 28-86), and the median Karnofsky Performance Scale was 80 (range: 60-100). The primary sites were colon (85 patients, 76.6%) and rectal (26 patients, 23.4%). Three patients underwent hypofractionated SRS (3 sessions) with a median margin dose of 27 Gy (range: 27-30). All other patients underwent single-session SRS with a median margin dose of 18 Gy (range: 13-20).

RESULTS:
The median patient survival after SRS was 7 months (range: 1-174). Ninety-eight (88.3%) patients expired at last follow-up and 15 patients (15.3%) died related to progressive intracranial disease. A Karnofsky Performance Scale of <80 at SRS presentation (P = .02, hazard ratio [HR]: 0.6, 95% CI: 0.4-0.9) and no previous surgical resection (P < .01, HR: 0.4, 95% CI: 0.3-0.7) were associated with inferior overall survival using multivariate analysis. Seventeen patients (15.3%) had documented local tumor progression after SRS, at a median time of 7 months (range: 3-34) between SRS and progression. Twenty-six patients (23.4%) developed new BMs at a median of 5 months (range: 2-26) between SRS and new tumor detection. Less than three BMs at SRS presentation (P = .02, HR: 2.6, 95% CI: 1.2-5.6) were associated with better distant tumor control on multivariate analysis. The incidence of adverse radiation effects was 5.4%.

CONCLUSION:
SRS effectively controls BMs from CRC with low risk of treatment-related toxicity. During follow-up, the development of additional metastases can be safely treated by repeat SRS.
BIOMECHANICS OF FACET JOINTS: SYNCHRONIZED SPATIO-TEMPORAL AND MECHANICAL ANALYSIS
Authors: Warren James , Mengoni Marlène 
Published: November 2023

AbstractObjectivesWhile spinal fusion is known to be associated with adjacent disc degeneration, little is known on the role of the facet joints in the process, and whether their altered biomechanics following fusion plays a role in further spinal degeneration. This work aimed to develop a model and method to sequentially measure the effects of spinal fusion on lumbar facet joints through synchronisation of both motion analysis, pressure mapping and mechanical analysis.MethodsParallel measurements of mature ovine lumbar facet joints (∼8yr old, n=3) were carried out using synchronised load and displacement measurements, motion capture during loading and pressure mapping of the joint spaces during loading. Functional units were prepared and cemented in PMMA endcaps. Displacement-controlled compression measurements were carried out using a materials testing machine (3365, Instron, USA) at 1 mm/min up to 950 N with the samples in a neutral position, while motion capture of the facet joints during compression was carried out using orthogonal HD webcams (Logitech, Switzerland) to measure the displacement of key facet joint features. The pressure mapping of load transfer during displacement was carried out using a flexible pressure sensor (6900 series, Tekscan, USA). Each sample was imaged at an isotropic resolution of 82 microns using a μCT scanner (XtremeCT, Scanco, Switzerland) to quantify the curvature within the facet joints.ResultsRelative facet joint displacement under load, in a neutral position, showed more displacement (2.36 ±1.68 mm) compared to the cross-head when under compression (2.06 ±1.19 mm). Motion capture indicated the relative displacement of the facet joints was more posterior with some lateral motion. For five of the six facet joints, pressure measurement was possible only on 24±7 % of the surface due to the large change in curvature. Partially measured loads through the facets was 10.5 ±1.1 N.ConclusionsThe relative displacement of the lumbar facet joints compared to the crosshead displacement was consistent with previous studies of cervical facet joints, despite the differences in anatomical geometry between cervical and lumbar joints. The difficulties in accurately measuring the load transfer through the facet joints was due to the age of the tissue and the degree of curvature of the facet joints. Synchronisation of the biomechanical data will provide a setup to assess the effect of interventions such as spinal fusion, with curvature-related issues unlikely to occur in human spines.Declaration of Interest(a) fully declare any financial or other potential conflict of interest