bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytogenetic manifestations of multiple myeloma heterogeneity
Authors: Pantou Dimitra , Rizou Helen , Tsarouha Haroula , Pouli Anastasia , Papanastasiou Kostas , Stamatellou Marina , Trangas Theoni , Pandis Nikos , Bardi Georgia 
Published: November 2004

AbstractTo investigate the genetic basis of the great heterogeneity observed in the clinical behavior of multiple myeloma (MM), a combined approach of G‐banding, interphase fluorescence in situ hybridization (FISH), and multicolor FISH (M‐FISH) was employed to analyze 70 samples from 53 patients with MM. G‐banding revealed abnormal karyotypes in 77% of the cases. The origin of 31 chromosome markers was identified or revised by M‐FISH. Combined metaphase karyotypic data and interphase FISH findings, using the immunoglobulin heavy‐chain (IGH), IGH/cyclin D1 gene (CCND1), and D13S319 probes, revealed chromosome abnormalities in all evaluated patients and marked inter‐ and intratumor cytogenetic heterogeneity in the investigated MM samples. Cytogenetically unrelated clones were detected in 26% of the cases, mostly MM evaluated at diagnosis, whereas cytogenetic clonal evolution, manifested as related clones in 20% of the cases, was associated with disease progression. Among the 14q32 rearrangements, present in 66% of the cases, at least three cytogenetic subsets could be identified: one with t(11;14), usually without 13q14 deletion; another with other IGH changes, often 13q14 deletion, and hypodiploid modal chromosome number; and a third without changes in 14q32 but with abnormalities of chromosome 17. The correlation found between cytogenetic and clinicopathologic characteristics provided support for the concept that general genomic features in conjunction with specific chromosome rearrangements define the malignant phenotype in the various subsets of MM. © 2004 Wiley‐Liss, Inc.
Cytogenetic features of de novo CD5‐positive diffuse large B‐cell lymphoma: Chromosome aberrations affecting 8p21 and 11q13 constitute major subgroups with different overall survival
Authors: Yoshioka Tomoko , Miura Ikuo , Kume Masaaki , Takahashi Naoto , Okamoto Masataka , Ichinohasama Ryo , Yoshino Tadashi , Yamaguchi Motoko , Hirokawa Makoto , Sawada Ken‐ichi , Nakamura Shigeo 
Published: November 2004

AbstractDe novo CD5‐positive diffuse large B‐cell lymphoma (CD5+DLBCL) is regarded as a different clinicopathological entity from CD5‐negative DLBCL (CD5−DLBCL) and mantle cell lymphoma (MCL). Because only a few published cytogenetic studies of de novo CD5+DLBCL are available, we investigated chromosomal changes in 23 Japanese patients who had de novo CD5+DLBCL. A characteristic of cytogenetic abnormalities in de novo CD5+DLBCL was a high incidence of chromosomal aberrations affecting 8p21 and 11q13. Major chromosomal breakpoints were concentrated at 8p21, 11q13, and 3q27. Patients with 8p21 aberrations showed aggressive clinical features, including advanced stage of disease, elevated serum LDH level, poor performance status, and an inferior survival curve compared with patients who had 11q13 changes (P = .043). Chromosomal abnormalities of both 8p21 and 11q13 were not observed in the same patient, and each abnormality showed different chromosomal gains and losses. These results indicate that de novo CD5+DLBCL may occur in previously unidentified subgroups that differ in their chromosomal abnormalities. The conflicting results of previous studies on prognosis may thus be explained in part by the differences in chromosomal changes. © 2004 Wiley‐Liss, Inc.
Genotype prediction using a dense map of SNPs
Authors: Evans David M. , Cardon Lon R. , Morris Andrew P. 
Published: November 2004

AbstractThe International Haplotype Mapping Project (HapMap) aims to characterize the distribution and extent of linkage disequilibrium (LD) throughout the human genome, thereby facilitating genome‐wide association analysis and the search for the genetic determinants of complex diseases. Implicit in the rationale behind the project is the expectation that hidden (unobserved) disease‐causing variants will be in significant LD with surrounding typed markers and will thus be amenable to detection using association‐based mapping approaches. In order to investigate the validity of this assumption, we examined more than 5,000 SNPs across a 10‐MB region of chromosome 20 in a sample of 96 unrelated African‐American and 96 unrelated Caucasian individuals. We treated observed loci as surrogates for hidden SNPs by pretending that individuals' genotypes were unknown. We then attempted to predict these genotypes at the surrogate hidden SNP by using information about LD in the region and genotypes at surrounding observed loci. Our method is based on finding the most likely genotype for each individual, given all possible haplotype pairs consistent with observed genotypes for that individual at surrounding loci, and given the frequencies of those haplotypes in an independent sample. Our method performs extremely well in predicting genotypes in areas of high LD. Furthermore, in areas of low LD, our method results in substantial gains in predictive accuracy as compared to pair‐wise strategies. These results suggest that pair‐wise tests of disease‐marker association may be inferior to multipoint methods, which take advantage of the information contained within multi‐locus haplotypes. Genet. Epidemiol. © 2004 Wiley‐Liss, Inc.
Magnetic resonance study of the influence of tissue damage and cortical reorganization on PASAT performance at the earliest stage of multiple sclerosis
Authors: Audoin Bertrand , Van Au Duong My , Ranjeva Jean‐Philippe , Ibarrola Danielle , Malikova Irina , Confort‐Gouny Sylviane , Soulier Elisabeth , Viout Patrick , Ali‐Chérif André , Pelletier Jean , Cozzone Patrick J. 
Published: November 2004

AbstractWe sought to determine the influence of tissue damage and the potential impact of cortical reorganization on the performance to the Paced Auditory Serial Addition Test (PASAT) in patients at the earliest stage of multiple sclerosis (MS). Magnetization transfer ratio (MTR) imaging and functional magnetic resonance imaging (fMRI) experiments using PASAT as paradigm were carried out in 18 patients with clinically isolated syndrome suggestive of MS (CISSMS) compared to 18 controls. MTR histogram analyses showed structural abnormalities in patients involving the normal‐appearing white matter (NAWM) but also the gray matter (GM). Mean PASAT scores were significantly lower in the group of patients taken as a whole, and were correlated with the mean NAWM MTR value. No correlation was observed between PASAT scores and GM MTR. However, in the subgroup of patients with normal PASAT performance (n = 9), fMRI showed larger activations in bilateral Brodmann area 45 (BA45) and right BA44 compared to that in controls (n = 18). In these areas with potentially compensatory reorganization, the whole group of patients (n = 18) showed significantly greater activation than controls (n = 18). Activation in the right BA45 was inversely correlated with the mean NAWM MTR and the peak position of GM MTR histograms of patients. This study indicates that even at the earliest stage of MS, cortical reorganization is present inside the executive system of working memory and could tend to limit the determinant functional impact of NAWM injury on the execution of the PASAT. Hum. Brain Mapping 24:216–228, 2005. © 2004 Wiley‐Liss, Inc.
Glossal angiomyoma: Imaging findings and endovascular treatment
Authors: Marden Franklin A. , Calilao Gabriel C. , Guzman Grace , Roy Soma Sinha 
Published: November 2004

AbstractBackground.An angiomyoma is an uncommon, benign tumor characterized by numerous vascular channels intermixed with bundles of smooth muscle cells. Oral manifestations are quite rare. We describe for the first time the CT, MRI, and angiographic imaging features and successful preoperative endovascular embolization of an angiomyoma of the tongue. The pathologic findings before and after embolization are also described.Methods and Results.A 25‐year‐old man was seen with a rapidly enlarging tongue mass. Imaging studies revealed the extent and hypervascular nature of this tumor. The diagnosis of angiomyoma was confirmed by histologic examination. Preoperative embolization proved to be helpful in the surgical management of this lesion.Conclusions.Angiomyoma should be considered in the differential diagnosis of any well‐circumscribed, hypervascular, soft tissue tumor in the mouth. In addition, endovascular embolization may be a useful adjunct that facilitates resection. © 2004 Wiley Periodicals, Inc. Head Neck 26: 1084–1088, 2004
Development of thermal flow simulation method for switch mode power supplies and its integration with electric circuit analysis (in the case of natural convection air cooled switch mode power supply)
Authors: Koizumi Katsuhiro , Joboji Akito , Nagahara Kuniaki , Ishizuka Masaru 
Published: November 2004

AbstractThis paper describes the development of a thermal flow simulation method for the design of electronic equipment. In the proposed sequence of analyses, the electric circuit analysis for estimating power loss in power semiconductor devices was integrated with the thermal analysis. The method was applied to the thermal design of a new switch mode power supply (SMPS). The analysis was carried out using a commercially available computational fluid dynamics (CFD) code. In reducing actual structural organizations of the printed circuit board (PCB) and the power semiconductor devices to simpler models, the method of experimental design (MED) was employed. Following the prescription of MED, the PCB was treated as a simple plate having the same thermal conductivity as epoxy resin, and the power semiconductor devices were modeled by hexahedral resistance network. The heat sources were a field effect transistor (FET) and a diode. Computation of power loss from the FET and the diodes is described and the accuracy of different estimation methods is discussed. The difference between measured and calculated temperature rises of the power semiconductor devices was found to be within approximately 10 K. © 2004 Wiley Periodicals, Inc. Heat Trans Asian Res, 33(8): 473–489, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20037
Differential expression of 37 selected genes in hormone‐refractory prostate cancer using quantitative taqman real‐time RT‐PCR
Authors: Fromont Gaëlle , Chene Laurent , Vidaud Michel , Vallancien Guy , Mangin Philippe , Fournier Georges , Validire Pierre , Latil Alain , Cussenot Olivier 
Published: November 2004

AbstractProgression of prostate cancer to androgen independence remains the primary obstacle to improved survival. The development of more effective treatments depends on our understanding of the molecular events associated with the hormone‐refractory stage. We quantified, among 90 screened genes, the expression of 37 target genes, using real‐time quantitative RT‐PCR. Gene expression was studied in 13 samples of HPRC compared to 33 clinically localised cancers and normal prostate tissue. We identify 19 genes with significant differential expression in HRPC compared to localised prostate cancer. Genes with decreased expression included receptors for growth factors, MMR genes and the serine protease hepsin. Analysis of increased gene expression confirmed the importance of AR upregulation and highlighted genes not previously linked to HRPC, including enzymes involved in steroid synthesis and the antiapoptotic factor survivin. Progression of prostate cancer to the hormone‐refractory state is associated with differential gene expression, which may prove useful for both understanding disease progression and the development of new therapeutic approaches. © 2004 Wiley‐Liss, Inc.
Constraint satisfaction using soft quantifiers
Authors: Yager Ronald R. 
Published: November 2004

AbstractFuzzy sets and other methods have been used to model a softening of constraints in constraint propagation (CP) problems. Here, we suggest an approach to the softening of the CP problem at the meta level, in the process used to aggregate the satisfactions to the individual constraints. We discuss the possibility of using soft quantiﬁers such as ‘most’ to guide the process of aggregating the satisfactions to the individual constraints. Use is made of the ability to represent these soft quantiﬁers by fuzzy sets and the ability to implement their authorized aggregation by the ordered weighted averaging operator. Copyright © 2004 John Wiley & Sons, Ltd.
Differentiation of human colon adenocarcinoma cells alters the expression and intracellular localization of annexins A1, A2, and A5
Authors: Guzmán‐Aránguez Ana , Olmo Nieves , Turnay Javier , Lecona Emilio , Pérez‐Ramos Pablo , de Silanes Isabel López , Lizarbe M. Antonia 
Published: November 2004

AbstractButyrate induces differentiation and alters cell proliferation in intestinal–epithelial cells by modulation of the expression of several genes. Annexins are a superfamily of ubiquitous proteins characterized by their calcium‐dependent ability to bind to biological membranes; their involvement in several physiological processes, such as membrane trafficking, calcium signaling, cell motility, proliferation, and differentiation has been proposed. Thus, we have analyzed changes in annexin A1 (AnxA1), annexin A2 (AnxA2), and annexin A5 (AnxA5) levels and localization in human colon adenocarcinoma cells differentiated by butyrate treatment or by culture in glucose‐free inosine‐containing medium. The acquired differentiated phenotype increased dipeptidyl peptidase‐IV (DPP‐IV) expression and alkaline phosphatase (ALP) activity, two well known brush border markers. Butyrate induces cell differentiation and growth arrest in BCS‐TC2, BCS‐TC2.2, HT‐29, and Caco‐2 cells, increasing the levels of AnxA1 and AnxA5, whereas AnxA2 decreases except in Caco‐2 cells. Inosine‐differentiated cells present increased amounts of the three studied annexins, as occurs in spontaneously differentiated Caco‐2 cells. AnxA2 down‐regulation is not due to proteasome activation and seems to be related to the butyrate‐induced cell proliferation arrest; AnxA1 and AnxA5 expression is growth‐state independent. AnxA1 and AnxA5 are mainly found in the cytoplasm while AnxA2 is localized underneath the plasma membrane in cell‐to‐cell contacts. Butyrate induces changes in subcellular localization towards a vesicle‐associated pattern. Human colon adenocarcinoma cell differentiation is associated with an up‐regulation of AnxA1, AnxA2, and AnxA5 and with a subcellular relocation of these proteins. No correlation between annexin levels and tumorigenicity was found. Up‐regulation of AnxA1 could contribute to the reported anti‐inflammatory effects of butyrate in colon inflammatory diseases. © 2004 Wiley‐Liss, Inc.
Spatio‐temporal dynamics at rDNA foci: Global switching between DNA replication and transcription
Authors: Pliss Artem , Koberna Karel , Večeřová Jaromíra , Malínský Jan , Mašata Martin , Fialová Markéta , Raška Ivan , Berezney Ronald 
Published: November 2004

AbstractWe have investigated the in situ organization of ribosomal gene (rDNA) transcription and replication in HeLa cells. Fluorescence in situ hybridization (FISH) revealed numerous rDNA foci in the nucleolus. Each rDNA focus corresponds to a higher order chromatin domain containing multiple ribosomal genes. Multi‐channel labeling experiments indicated that, in the majority of cells, all the rDNA foci were active in transcription as demonstrated by co‐localization with signals to transcription and fibrillarin, a protein involved in ribosomal RNA processing. In some cells, however, a small portion of the rDNA foci did not overlap with signals to transcription and fibrillarin. Labeling for DNA replication revealed that those rDNA foci inactive in transcription were restricted to the S‐phase of the cell cycle and were replicated predominantly from mid to late S‐phase. Electron microscopic analysis localized the nucleolar transcription, replication, and fibrillarin signals to the dense fibrillar components of the nucleolus and at the borders of the fibrillar centers. We propose that the rDNA foci are the functional units for coordinating replication and transcription of the rRNA genes in space and time. This involves a global switching mechanism, active from mid to late S‐phase, for turning off transcription and turning on replication at individual rDNA foci. Once all the rRNA genes at individual foci are replicated, these higher order chromatin domains are reprogrammed for transcription. © 2004 Wiley‐Liss, Inc.
S19 ribosomal protein dimer augments metal‐induced apoptosis in a mouse fibroblastic cell line by ligation of the C5a receptor
Authors: Nishiura Hiroshi , Tanase Sumio , Shibuya Yoko , Futa Noriko , Sakamoto Tamami , Higginbottom Adrian , Monk Peter , Zwirner Jörg , Yamamoto Tetsuro 
Published: November 2004

AbstractTo analyze the role of S19 ribosomal protein (RP S19) in apoptosis, murine NIH3T3 were transfected with either hemagglutinin peptide‐tagged (HA) wild‐type human RP S19 or a mutant (Gln137Asn) that is resistant to transglutaminase‐catalyzed cross‐linked‐dimerization. Transfection with the mutant HA‐RP S19 inhibited manganese (II) (Mn II)‐induced apoptosis whereas the wild‐type HA‐RP S19 augmented apoptosis and a mock transfection had no effect. Release of the wild‐type HA‐RP S19 dimer but not the mutant HA‐RP S19 was observed during the apoptosis. The reduced rate of apoptosis of the cells transfected with the mutant HA‐RP S19 was overcome by addition of extracellular wild‐type RP S19 dimer. The apoptosis rates in cells transfected with either form of human HA‐RP S19 and in mock transfectants were reduced to about 40% by the presence of anti‐RP S19 antibody in the culture medium. Immunofluorescence staining and fluorescence‐activated cell sorting (FACS) analysis showed that the cell surface expression of the receptor for cross‐linked RP S19 dimer, C5a receptor, increased during apoptosis, concomitant with phosphatidylserine exposure. The expression of the C5a receptor gene also increased twofold. Apoptosis rates in the transfected and control cell lines were also reduced by the presence of an anti‐mouse C5a receptor monoclonal antibody or of a peptide C5a receptor antagonist. These results indicated the presence of an RP S19 dimer‐ and C5a receptor‐mediated autocrine‐type augmentation mechanism during Mn II‐induced apoptosis in the mouse fibroblastic cell line. In contrast to the RP S19 dimer, C5a actually inhibited apoptosis, suggesting that signaling through the C5a receptor varies depending on the ligand bound. © 2004 Wiley‐Liss, Inc.
Peroxisome proliferator‐activated receptor γ gene polymorphism is associated with serum triglyceride levels and body mass index in Japanese type 2 diabetic patients
Authors: Maeda Atsuko , Gohda Tomohito , Funabiki Kazuhiko , Horikoshi Satoshi , Tomino Yasuhiko 
Published: November 2004

AbstractPeroxisome proliferator‐activated receptor γ (PPARγ) controls adipocyte differentiation and regulates lipid and glucose homeostasis. Therefore, the PPARγ gene may affect insulin sensitivity and resistance. We analyzed the relationship between C/T exon 6 polymorphism of the PPARγ gene and various clinical parameters in type 2 diabetic patients. There were no significant differences in the frequencies of genotype and allele between diabetic patients with and without nephropathy. Diabetic patients were divided into two groups: patients bearing at least one T allele (CT/TT), and patients with no T allele (CC). Levels of serum triglyceride and body mass index (BMI) were significantly higher in the CT/TT genotype group than in the CC genotype group. Since obesity affects insulin resistance, the diabetic patients were also divided into two groups: those with a BMI of <23, and those with a BMI of >23. In patients with a BMI of <23, there was no significant change in the levels of glycosylated hemoglobin A1c (HbA1c) between the CC and CT/TT genotype groups. However, in patients with a BMI of >23, HbA1c levels were significantly higher in the CT/TT genotype group than in the CC genotype group. It appears that the CT/TT genotype with PPARγ gene polymorphism may contribute to higher BMI and higher serum triglyceride and HbA1c levels in Japanese type 2 diabetic patients. J. Clin. Lab. Anal. 18:317–321, 2004. © 2004 Wiley‐Liss, Inc.
Early viral kinetics and treatment outcome in combination of high‐dose interferon induction vs. pegylated interferon plus ribavirin for naïve patients infected with hepatitis C virus of genotype 1b and high viral load
Authors: Tsubota Akihito , Arase Yasuji , Someya Takashi , Suzuki Yoshiyuki , Suzuki Fumitaka , Saitoh Satoshi , Ikeda Kenji , Akuta Norio , Hosaka Tetsuya , Kobayashi Masahiro , Kumada Hiromitsu 
Published: November 2004

AbstractAn investigation was carried out to determine whether early viral monitoring could predict efficiently the virological response to combination therapy of two different regimens in treatment‐naïve chronic hepatitis C patients infected with genotype 1b with high baseline viral load. Patients were randomly assigned to receive interferon (IFN) alpha‐2b induction (6 MU daily for 2 weeks) followed by 6 MU thrice weekly for 46 weeks (IFN/R group; n = 20), or pegylated IFN alpha‐2b (1.5 μg/kg) weekly for 48 weeks (PEG/R group; n = 28), in combination with ribavirin (600‐1,000 mg daily). Serum HCV RNA was quantitated at 0, 6, 12, 24, and 48 hr post‐dose, weekly during the first 4 weeks, and thereafter viral kinetics were assessed every 4 weeks. The sustained virological response rates in the IFN/R and PEG/R groups were 40% (8/20) and 43% (12/28), respectively. The non‐virological response rates were 40% (8/20) and 39% (11/28), respectively. The cumulative virological response rates were similar in both groups. Multivariate analyses identified no independent baseline variables linked to sustained virological or non‐virological response. Early log viral load changes from baseline in both groups were significantly greater at all time‐points after 24 hr in virological response patients than in non‐virological response patients (P < 0.001 for all). On the receiver operating characteristics curves for prediction of non‐virological response, the area under the curves (0.951–1.000), sensitivity (90%–100%), and negative predictive value (96%– 100%) were similar at any time‐points after 24 hr. For prediction of sustained virological response, sensitivity of 80% with 86% negative predictive value was observed for negative HCV RNA at week 12, with the highest area under the curves value of 0.919. The results suggest that early monitoring of viral kinetics is a useful measure to predict virological response, and might facilitate development of rational and effective therapeutic strategies. J. Med. Virol. 75:27–34, 2005. © 2005 Wiley‐Liss, Inc.