bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic LMP1 polyepitope vaccine for EBV-associated Hodgkin disease and nasopharyngeal carcinoma
Authors: Duraiswamy Jaikumar , Sherritt Martina , Thomson Scott , Tellam Judy , Cooper Leanne , Connolly Geoff , Bharadwaj Mandvi , Khanna Rajiv 
Published: April 2003

AbstractDevelopment of an epitope-based vaccination strategy designed to enhance Epstein-Barr virus (EBV)–specific CD8+cytotoxic T lymphocytes (CTLs) is increasingly being considered as a preferred approach for the treatment of EBV-associated relapsed Hodgkin disease (HD) and nasopharyngeal carcinoma (NPC). EBV-encoded latent membrane proteins, LMP1 and LMP2, are the only target antigens available for therapeutic augmentation of CTL responses in patients with HD and NPC. Here, we describe preclinical studies using a recombinant poxvirus vaccine that encodes a polyepitope protein comprising 6 HLA A2–restricted epitopes derived from LMP1. Human cells infected with this recombinant polyepitope construct were efficiently recognized by LMP1-specific CTL lines from HLA A2 healthy individuals. Furthermore, immunization of HLA A2/Kb mice with this polyepitope vaccine consistently generated strong LMP1-specific CTL responses to 5 of the 6 epitopes, which were readily detected by both ex vivo and in vitro assays. More important, this polyepitope vaccine successfully reversed the outgrowth of LMP1-expressing tumors in HLA A2/Kb mice. These studies provide an important platform for the development of an LMP-based polyepitope vaccine as an immunotherapeutic tool for the treatment of EBV-associated HD and NPC.
Subexponential asymptotics of a Markov-modulated random walk with queueing applications
Authors: Jelenković Predrag R. , Lazar Aurel A. 
Published: April 2003

Let {(Xn,Jn)} be a stationary Markov-modulated random walk on ℝ x E (E is finite), defined by its probability transition matrix measure F = {Fij}, Fij(B) = ℙ[X1 ∈ B, J1 = j | J0 = i], B ∈ B(ℝ), i, j ∈ E. If Fij([x,∞))/(1-H(x)) → Wij ∈ [0,∞), as x → ∞, for some long-tailed distribution function H, then the ascending ladder heights matrix distribution G+(x) (right Wiener-Hopf factor) has long-tailed asymptotics. If 𝔼Xn < 0, at least one Wij > 0, and H(x) is a subexponential distribution function, then the asymptotic behavior of the supremum of this random walk is the same as in the i.i.d. case, and it is given by ℙ[supn≥0Sn > x] → (−𝔼Xn)−1 ∫x∞ ℙ[Xn > u]du as x → ∞, where Sn = ∑1nXk, S0 = 0. Two general queueing applications of this result are given.First, if the same asymptotic conditions are imposed on a Markov-modulated G/G/1 queue, then the waiting time distribution has the same asymptotics as the waiting time distribution of a GI/GI/1 queue, i.e., it is given by the integrated tail of the service time distribution function divided by the negative drift of the queue increment process. Second, the autocorrelation function of a class of processes constructed by embedding a Markov chain into a subexponential renewal process, has a subexponential tail. When a fluid flow queue is fed by these processes, the queue-length distribution is asymptotically proportional to its autocorrelation function.
Explicit sufficient invariants for an interacting particle system
Authors: Itoh Yoshiaki , Mallows Colin , Shepp Larry 
Published: April 2003

We introduce a new class of interacting particle systems on a graphG. Suppose initially there areNi(0) particles at each vertexiofG, and that the particles interact to form a Markov chain: at each instant two particles are chosen at random, and if these are atadjacentvertices ofG, one particle jumps to the other particle's vertex, each with probability 1/2. The processNenters a death state after a finite time when all the particles are in someindependentsubset of the vertices ofG, i.e. a set of vertices with no edges between any two of them. The problem is to find the distribution of the death state, ηi=Ni(∞), as a function ofNi(0).We are able to obtain, for some special graphs, thelimitingdistribution ofNiif the total number of particlesN→ ∞ in such a way that the fraction,Ni(0)/S= ξi, at each vertex is held fixed asN→ ∞. In particular we can obtain the limit law for the graphS2, the two-leaf star which has three vertices and two edges.
A minimax optimal stop rule in reliability checking
Authors: Gouweleeuw José M. 
Published: April 2003

Consider a machine, which may or may not have a defect, and the probability q that this machine is defective is unknown. In order to determine whether the machine is defective, it is tested. On each test, the defect is found with probability p, if it has not been found yet. Performing n tests costs cn dollars and there is a fine of 1 dollar if there is a defect and it is not found on the tests. When should we stop testing, in order to minimize the cost?This problem is treated in a minimax setting: we try to find a strategy that works well, even for ‘bad’ q's. It turns out that the minimax optimal stop rule can be unexpectedly complicated. For example, if p = 1/2 and cn = cn = 0.25n, then the optimal rule is to start by performing one test. If a defect is found we stop, otherwise we perform a second test. If a defect is found, then again we stop, else toss a coin and stop if this shows heads. If we still have not stopped, a third and last test is performed.
Sequential selection of an increasing subsequence from a sample of random size
Authors: Gnedin Alexander V. 
Published: April 2003

A random number of independent identically distributed random variables is inspected in strict succession. As a variable is inspected, it can either be selected or rejected and this decision becomes final at once. The selected sequence must increase. The problem is to maximize the expected length of the selected sequence.We demonstrate decision policies which approach optimality when the number of observations becomes in a sense large and show that the maximum expected length is close to an easily computable value.
On a distributed clustering process of Coffman, Courtois, Gilbert and Piret
Authors: van den Berg J. , Ermakov A. 
Published: April 2003

Coffman, Courtois, Gilbert and Piret (1991) have introduced a flow process in graphs, where each vertex gets an initial random resource, and where at each time vertices with large resources tend to attract resources from neighbours. The initial resources are assumed to be i.i.d., with a continuous distribution.We are mainly interested in the following question: does, with probability 1, the resource of each vertex change only finitely many times?Coffman et al. concentrate mainly on the case where the graph corresponds with the integer points on the line, in which case it is easily seen that the answer to the above question is positive. For higher-dimensional lattices they make general remarks which suggest that the answer to the above question is still positive. However, no proof seems to be known.We restrict to the case of the square lattice, and, by a percolation approach, we reduce the question above to the question whether a certain quantity, which can be obtained from a finite computation, is sufficiently small. This computation is, however, still too long to be executed in an acceptable time. We therefore apply Monte Carlo simulation for this finite problem, which gives overwhelming evidence that, for the square lattice, the answer to the main question is positive.
Evidence of a novel transduction pathway mediating detection of polyamines by the zebrafish olfactory system
Authors: Michel W. C. , Sanderson M. J. , Olson J. K. , Lipschitz D. L. 
Published: April 2003

SUMMARY
To better understand the full extent of the odorant detection capabilities of fish, we investigated the olfactory sensitivity of zebrafish to a monoamine and several polyamines using electrophysiological and activity-dependent labeling techniques. Electro-olfactogram (EOG) recording methods established the relative stimulatory effectiveness of these odorants as: spermine &gt;&gt;spermidine ≈ agmatine &gt; glutamine &gt; putrescine ≥ cadaverine ≥histamine &gt; artificial freshwater. The detection threshold for the potent polyamines was approximately 1 μmol l–1. Cross-adaptation experiments suggested that multiple receptors are involved in polyamine detection. Three observations indicated that polyamine signaling may involve a transduction cascade distinct from those used by either amino acids or bile salts. Like bile salts and the adenylate cyclase activator forskolin, but unlike amino acid odorants, polyamines failed to stimulate activity-dependent labeling of olfactory sensory neurons with the cation channel permeant probe agmatine, suggesting a signaling pathway different from that used by amino acid stimuli. Also supporting distinct amino acid and polyamine signaling pathways is the finding that altering phospholipase C activity with the inhibitor U-73122 significantly reduced amino acid-evoked responses, but had little effect on polyamine- (or bile salt-) evoked responses. Altering cyclic nucleotide-mediated signaling by adenylate cyclase activation with forskolin,which significantly reduced responses to bile salts, failed to attenuate polyamine responses, suggesting that polyamines and bile salts do not share a common transduction cascade. Collectively, these findings suggest that polyamines are a new class of olfactory stimuli transduced by a receptor-mediated, second messenger signaling pathway that is distinct from those used by amino acids or bile salts.
Liposome Immobilization on Polymer Gel Particles by in situ Formation of Covalent Linkages
Authors: Khaleque Md Abdul , Okumura Yukihisa , Yabushita Satoshi , Mitani Michiharu 
Published: April 2003

Abstract
In situ formation of disulfide linkages between liposomes and Sephacryl gel particles both of that were bearing mercapto moieties was demonstrated. The reaction led to immobilization of the liposomes on the gel. The immobilized liposomes could be detached by reductive treatment of the liposome-gel conjugate, and the resulting gel was still capable of further immobilizing liposomes.
Total Synthesis of (±)-Aiphanol, a Novel Cyclooxygenase-inhibitory Stilbenolignan
Authors: Kuboki Atsuhito , Yamamoto Toru , Ohira Susumu 
Published: April 2003

Abstract
(±)-Aiphanol has been stereoselectively synthesized through IBX-mediated o-quinone formation, and through [4+2] cycloaddition of the o-quinone and cinnamyl alcohol unit.
Novel Monocation, Dication, and Dianion of a Cage Molecule, Trithienylmethanophane
Authors: Kurata Hiroyuki , Haruki Kenji , Nakaminami Hiromichi , Kawase Takeshi , Oda Masaji 
Published: April 2003

Abstract
The McMurry coupling of tris(5-formyl-2-thienyl)methane afforded an unsubstituted, etheno-bridged trithienylmethanophane which was in turn transformed to novel cage-molecular monocation, dication, and dianion of substantial stability. The latter two species are the first examples of fully conjugated ionic cyclophane cage molecule.
Holt‐Oram syndrome: Is there a “face”?
Authors: Allanson Judith E. , Newbury‐Ecob Ruth A. 
Published: April 2003

AbstractHolt‐Oram syndrome, first described in 1960, is one of many heart‐hand syndromes. Upper limb involvement, predominantly radial, is universal, bilateral and asymmetrical, with variable severity. Cardiac defects occur in 95% of familial cases. Inheritance is autosomal dominant with 100% penetrance and no evidence of reduced fitness. Mutations in TBX5 have been reported in Holt‐Oram syndrome. This study was conducted to establish whether a particular facial appearance is associated with Holt‐Oram syndrome, one which might facilitate early diagnosis and aid differentiation from other heart‐hand syndromes. Twenty‐five individuals were evaluated, age 11 months to 70 years. A complete dysmorphological examination was carried out, serial photographs were reviewed, and a series of anthropometric craniofacial measurements was obtained. Subjectively, the face is square with a broad lower jaw and parietal bossing. The forehead is prominent and tall. There is narrowing at the temples. Eyes seem close‐set. The nose appears relatively long, with a wide base, and short columella. With age the face becomes longer and more oval. Our anthropometric approach confirms certain clinical impressions. However, there is no objective evidence for increased face or nose height, two of the most striking features of the “gestalt.” Nasal height is, in fact, reduced at all ages. There does not appear to be a syndrome‐specific pattern profile to facilitate the discrimination of this condition from other heart‐hand syndromes. © 2003 Wiley‐Liss, Inc.
Tetrasomy 8p: Discordance of amniotic fluid and blood karyotypes
Authors: López‐Pajares I. , Delicado A. , Lapunzina P. , Mori M.A. , de Torres M.L. , Aso S. , Garcia Sanchez P. 
Published: April 2003

AbstractWe describe a girl with congenital heart defect (ventricular septal defect), facial, ear and bone anomalies, agenesis of corpus callosum and conventional cytogenetic studies showing tetrasomy 8p. The identity of the isochromosome was confirmed by fluorescent in situ hybridization (FISH) using painting, subtelomeric and alpha satellite probes for chromosome 8. The extra isochromosome was observed in 100% of cultured peripheral lymphocytes (47,XX,+i(8)(p10)), but normal chromosomes were recorded in cultured amniotic fluid. Microsatellites analysis of the patient's DNA with two markers mapping 8p showed three different peaks, and two markers mapping 8q showed two peaks. To the best of our knowledge, this patient represents the twelfth reported case of tetrasomy 8p. In addition, our report is the first case with a pure tetrasomy 8p in blood, (the other published cases are mosaic 8p), and the second case with a discordance of amniotic fluid and blood karyotypes [Robinow et al., 1989: Am J Med Genet 32:320–324]. © 2003 Wiley‐Liss, Inc.
Late‐Onset visceral presentation with cardiomyopathy and without neurological symptoms of adult Sanfilippo A syndrome
Authors: Van Hove Johan L.K. , Wevers Ron A. , Van Cleemput Johan , Moerman Philippe , Sciot Raf , Matthijs Gert , Schollen Els , de Jong Jan G.N. , Carey William F. , Muller Viv , Nicholls Cath , Perkins Kelly , Hopwood John J. 
Published: April 2003

AbstractSanfilippo A syndrome, mucopolysaccharidosis type IIIA, is caused by a deficiency of heparan sulphamidase activity, and usually presents in childhood with neurodegeneration leading to death in teenage years. Visceral symptoms are limited to coarsening and diarrhea. We now describe an adult patient who presented with cardiomyopathy. At age 45 years she had hypertension, and the next year she developed a progressively worsening cardiomyopathy with prominent apical hypertrophy and atrial fibrillation. At age 53, she had severe concentric hypertrophic nonobstructive cardiomyopathy in both ventricles. There was no coarsening of features. Neurologic function, skeleton, cornea, liver, and spleen were normal. Percutaneous endomyocardial biopsy showed ballooned cardiomyocytes with storage vacuoles, containing acid mucopolysaccharides. Leucocytes, uterus, and brain biopsy did not show this storage material. There was a slight increase in total urine mucopolysaccharides, with an increased proportion of heparan sulfates. Heparan sulphamidase activity was deficient in leukocytes and heparan sulphamidase protein and activity were reduced in cultured fibroblasts. No mutations were identified after sequencing of the heparan sulphamidase gene at the cDNA and the genomic level. This new clinical presentation expands the clinical spectrum of Sanfilippo A syndrome to include a primary visceral presentation of cardiomyopathy without neurologic symptoms in the adult. The late onset may be related to the residual heparan sulphamidase activity. The genetic basis of this new variant is still unclear. Physicians evaluating adults must remain aware of possible new adult presentations of storage conditions. © 2003 Wiley‐Liss, Inc.
Haplotypes of α‐globin gene regulatory element in two Brazilian native populations
Authors: Ribeiro Daniela M. , Figueiredo Maria S. , Costa Fernando F. , Sonati Maria F. 
Published: April 2003

AbstractThe α‐major regulatory element (α‐MRE), located 40 Kb far upstream of the α‐globin gene cluster on chromosome 16, is involved in the regulation of human α‐globin genes expression. The activity of this element is restricted to a 350‐bp fragment in which several nuclear protein binding sites have been identified. This element is genetically polymorphic and different haplotypes, named A–F, have been determined in seven populations of Europe, Africa, Asia, and Oceania. We describe here the α‐MRE haplotypes found in native Indians from two nonmiscegenated tribes of the north region of Brazil, in Amazonia, the Parakanã and the Xikrin. The A haplotype was predominant in both (70% and 87%, respectively), followed by the B haplotype (30% and 13%, respectively). The haplotype frequency distribution among the Parakanã was similar to that reported for Indonesians and Southeast Asian populations, while the distribution among the Xikrin showed higher similarity to that observed in Indonesians. These results corroborate the existence of genetic affinities between Brazilian Indians and Southeast Asian and Oceanic populations. This was the first investigative work on the α‐MRE polymorphism in South American native populations in general or Brazilian native populations in particular. Am J Phys Anthropol 121:000–000, 2003. © 2003 Wiley‐Liss, Inc.
Syndiotactic polymerization of styrene catalyzed by alkenyl‐substituted cyclopentadienyltitanium trichlorides
Authors: Qian Xianmiao , Huang Jiling , Qian Yanlong , Wang Chen 
Published: April 2003

AbstractCH2CHCH2CpTiCl3 (1), CH2CHCH2CH2CpTiCl3 (2) and CH3CH2CH3CpTiCl3 (3) have been synthesized and characterized. The influence of the alkenyl substituent groups on the catalyst activities in the syndiotactic polymerization of styrene was investigated. The catalyst activities decreased in the order CH2CHCH2CH2CpTiCl3 > CH3CH2CH2CH2CpTiCl3 > CH3CH2CH2CpTiCl3 > CH2CHCH2CpTiCl3 (CpC5H4). By using complex 1, the dependence of the activity on the concentration of methylaluminoxane, triisobutylaluminum and diisobutylaluminum hydride was investigated. Copyright © 2003 John Wiley & Sons, Ltd.
Transients from a mixture of [(R(O)Sn)2C(CH3)2]2 and (RCl2Sn)2C(CH3)2 [R = (SiMe3)2CH]: an identification in situ by 1D 119Sn and 2D 1H119Sn HMQC NMR spectroscopy and electrospray mass spectrometry
Authors: Biesemans Monique , Duthie Andrew , Jurkschat Klaus , Verbruggen Ingrid , Willem Rudolph , Zobel Bernhard 
Published: April 2003

AbstractThe reaction of the 2,2‐bis(organodichlorostannyl)propane [(Me3Si)2CH(Cl2)Sn]2CMe2 (A) with the corresponding organotin oxide {[(Me3Si)2CH(O)Sn]2CMe2}2 (B) does not provide the corresponding normally expected tetraorganodistannoxane {[(Me3Si)2CH(Cl)SnCMe2Sn(Cl)CH(SiMe3)2]O}n but a complex reaction mixture. One major product, namely the 2,4,6,8‐tetraorgano‐2,6‐dichloro‐1,5,9‐trioxa‐2,4,6,8‐tetrastannabicyclo[3.3.1]nonane derivative [(Me3Si)2CHSnCMe2Sn(Cl)CH(SiMe3)2]2O3 (C) was identified in situ by 2D 1H119Sn and 1H13C heteronuclear multiple quantum coherence and heteronuclear multiple bond correlation NMR spectroscopy as well as electrospray mass spectrometry. Compound C is proposed to be in equilibrium with an ionic species C′, the cation of which has an adamantane‐type structure. Copyright © 2003 John Wiley & Sons, Ltd.
Properties of polyacrylate soap‐free hydrosol with zirconium chelate
Authors: Huang Zhi Hong , Xie Zhi Ming , Li Zhuo Mei 
Published: April 2003

AbstractThe effect of the amount of triethanolamine zirconium isopropyloxide chelate in acrylate terpolymer soap‐free hydrosol on the properties of hydrosol and its film was studied. The addition of the chelate increased the hydrosol viscosity to 20 mPas and improved the hydrosol film water resistance; the water uptake was reduced to 0.6%, and the hydrosol still retained good resolubility. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 88: 2858–2860, 2003
Kinetics of amide formation on 1,4‐butanediol dimethacrylate crosslinked polystyrene resin: A comparison with PS‐DVB resin
Authors: Roice M. , Pillai V. N. Rajasekharan 
Published: April 2003

AbstractAn efficient crosslinked polymer support was synthesized by introducing 1,4‐butanediol dimethacrylate crosslinker to a polystyrene network using aqueous suspension polymerization technique. The support was functionalized with aminomethyl groups using chloromethyl phthalimide. The kinetics of amide bond formation on 1,4‐butanediol dimethacrylate crosslinked polystyrene (PS‐BDODMA) polymer were carried out using the attachment of Rink amide linker as a model reaction. The efficiency of the support was tested and compared with Merrifield resin by following different steps involved in the synthesis of a 14‐residue model peptide, Lys‐Ile‐Asn‐Thr‐Asn‐Ala‐Ser‐Trp‐His‐Ala‐Asn‐Arg‐Thr‐Ala‐NH2, under the same synthetic conditions. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 88: 2897–2903, 2003
Synthesis of new chelating ion exchange resin with hydroxamic and amidoxime groups and study of its metal binding property
Authors: Neagu Violeta , Bunia I. , Plesca I. , Popa M. 
Published: April 2003

AbstractThe synthesis of a new chelating ion exchanger with hydroxamic and amidoxime groups was performed by an aminolysis reaction of an ethylacrylate/acrylonitrile/divinylbenzene copolymer with hydroxylamine in the presence of sodium ethoxylate. We studied the retention property of the new resin for Pb(II), Cd(II), Cu(II), Ni(II), Zn(II), Mn(II), and Fe(III) metal cations from aqueous solutions. The retention capacity depends on the contact time, the pH of the solution, and the types of counterions of the metal cations. Competitive adsorptions of Pb(NO3)2 + Fe2(SO4)3, Pb(NO3)2 + MnSO4, Pb(NO3)2 + Cd(NO3)2, and Pb(NO3)2 + CuCl2 on the new chelating ion exchanger were performed. The results showed that the resin preferentially retained the Pb(II) ions from the binary aqueous mixtures. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 88: 2956–2962, 2003
Dual lower critical solution temperature polymer networks based on block, laminate, and interpenetrating network structures composed of N‐alkylacrylamides and N,N‐dialkylaminoethyl methacrylates
Authors: Stoltz Michael J. , Brazel Christopher S. 
Published: April 2003

AbstractNovel materials that display two lower critical solution temperatures (LCSTs) were developed by forming block copolymers, laminate structures, and interpenetrating networks of crosslinked polymer systems that displayed temperature sensitivity independently. A number of LCST polymers and copolymers were investigated, including those based on N‐isopropylacrylamide, N,N‐diethylacrylamide, N,N‐diethylaminoethyl methacrylate, and N,N‐dimethylaminoethyl methacrylate. The polymer structure was found to profoundly influence the thermal sensitivity, as polymer formulation techniques led to materials with varying degrees of temperature sensitivity. Random and block copolymerization, along with interpenetrating networks and laminate systems, were studied, with only the structures having the greatest physical separation between pendent chains of different types having the ability to separate temperature transitions. Experiments were conducted to characterize the equilibrium swelling behavior and thermal transitions in the polymer systems. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 88: 2974–2981, 2003