bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hubungan Tingkat Pendidikan Terhadap Kepercayaan Diri Perawat Dalam Melakukan CPR di IGD RSD X
Authors: Muthmainnah Muthmainnah , Maulani Firman 
Published: January 2024

Latar Belakang: henti jantung merupakan kondisi yang dapat mengakibatkan sesorang meninggal. Kematian akibat henti jantung merupakan penyumbang kematian mendadak terbesar di dunia.
17,9 juta orang meninggal akibat penyakit kardiovaskuler pada tahun 2019, mewakili 32% dari semua kematian global. Dari kematian tersebut, 85% disebabkan oleh serangan jantung dan stroke (World Health Association (WHO), 2021). Di Amerika Serikat, angka kejadian In Hospital Cardiac Arrest (IHCA) diperkirakan sekitar 200 ribu orang setiap tahunnya dan yang bisa diselamatkan hanya kurang dari 20%.
Tujuan: Tujuan penelitian ini untuk mengetahui hubungan tingkat pendidikan terhadap kepercayaan diri perawat dalam melakukan CPR di IGD.
Metode: Penelitian ini merupakan pendekatan penelitian korelatif cross sectional. Teknik pengambilan sampel menggunakan teknik sampling jenuh dengan jumlah responden sebanyak 32 orang. Instrumen penelitian berupa angket dan uji statistik menggunakan spearman rank. Hasil : Hasil statistik uji peringkat spearman menunjukkan bahwa pendidikan (p=0,046) sehingga dapat disimpulkan bahwa terdapat hubungan antara jenjang pendidikan dengan kepercayaan diri perawat yang memberikan CPR di IGD
Kesimpulan: Hasil dari kekuatan hubungan tersebut kuat dimana semakin tinggi tingkat pendidikan dan semakin semakin tinggi kepercayaandiri perawat memberikan CPR.
In vitro biocompatibility evaluation of functional electrically stimulating microelectrodes on primary glia
Authors: Tsui Christopher T. , Mirkiani Soroush , Roszko David A. , Churchward Matthew A. , Mushahwar Vivian K. , Todd Kathryn G. 
Published: January 2024

Neural interfacing devices interact with the central nervous system to alleviate functional deficits arising from disease or injury. This often entails the use of invasive microelectrode implants that elicit inflammatory responses from glial cells and leads to loss of device function. Previous work focused on improving implant biocompatibility by modifying electrode composition; here, we investigated the direct effects of electrical stimulation on glial cells at the electrode interface. A high-throughput in vitro system that assesses primary glial cell response to biphasic stimulation waveforms at 0 mA, 0.15 mA, and 1.5 mA was developed and optimized. Primary mixed glial cell cultures were generated from heterozygous CX3CR-1+/EGFP mice, electrically stimulated for 4 h/day over 3 days using 75 μm platinum-iridium microelectrodes, and biomarker immunofluorescence was measured. Electrodes were then imaged on a scanning electron microscope to assess sustained electrode damage. Fluorescence and electron microscopy analyses suggest varying degrees of localized responses for each biomarker assayed (Hoescht, EGFP, GFAP, and IL-1β), a result that expands on comparable in vivo models. This system allows for the comparison of a breadth of electrical stimulation parameters, and opens another avenue through which neural interfacing device developers can improve biocompatibility and longevity of electrodes in tissue.
Sacubitril/valsartan attenuates atrial conduction disturbance and electrophysiological heterogeneity with ameliorating fibrosis in mice
Authors: Iwamiya Satoshi , Ihara Kensuke , Furukawa Tetsushi , Sasano Tetsuo 
Published: January 2024

BackgroundSacubitril/valsartan (SacVal) has been shown to improve the prognosis of heart failure; however, whether SacVal reduces the occurrence of atrial fibrillation (AF) in heart failure has not yet been elucidated. In this study, we aimed to determine whether SacVal is effective in reducing the occurrence of AF in heart failure and identify the underlying mechanism of its electrophysiological effect in mice.MethodsAdult male mice underwent transverse aortic constriction, followed by SacVal, valsartan, or vehicle treatment for two weeks. Electrophysiological study (EPS) and optical mapping were performed to assess the susceptibility to AF and the atrial conduction properties, and fibrosis was investigated using heart tissue and isolated cardiac fibroblasts (CFs).ResultsEPS analysis revealed that AF was significantly less inducible in SacVal-treated mice than in vehicle-treated mice. Optical mapping of the atrium showed that SacVal-treated and valsartan-treated mice restored the prolonged action potential duration (APD); however, only SacVal-treated mice showed the restoration of decreased conduction velocity (CV) compared to vehicle-treated mice. In addition, the electrophysiological distribution analysis demonstrated that heterogeneous electrophysiological properties were rate-dependent and increased heterogeneity was closely related to the susceptibility to AF. SacVal attenuated the increased heterogeneity of CV at short pacing cycle length in atria, whereas Val could not. Histological and molecular evaluation showed that SacVal exerted the anti-fibrotic effect on the atria. An in vitro study of CFs treated with natriuretic peptides and LBQ657, the metabolite and active form of sacubitril, revealed that C-type natriuretic peptide (CNP) combined with LBQ657 had an additional anti-fibrotic effect on CFs.ConclusionsOur results demonstrated that SacVal can improve the conduction disturbance and heterogeneity through the attenuation of fibrosis in murine atria and reduce the susceptibility of AF in heart failure with pressure overload, which might be attributed to the enhanced function of CNP.
Study on the actual particle size, activity concentration, and migration process adsorption behavior of radioactive substances in liquid effluents from nuclear power plants
Authors: Jiang Zhenyu , Xiong Jun , Pan Yuelong , Hu Jie , Chen Yujia , Yin Shuhua , Yan Yihong 
Published: January 2024

Radionuclides emitted by nuclear power plants may have effects on the environment and public health. At present, research on radioactive material effluent in the industry mainly focuses on the treatment of radioactive effluent and the particle size distribution of the primary circuit. There is little research on the particle size of radioactive material during the migration process outside the primary circuit system, as well as the flocculation precipitation and other enrichment phenomena during the collection process of effluent. Therefore, this study relies on the sampling of effluent from an in-service nuclear power plant to measure its radioactivity level by particle size range. At the same time, the mixing process of effluent is simulated in the laboratory to simulate the adsorption behavior of effluent during the migration process. It was found that in the activity concentration of detectable radioactive nuclides in the effluent samples, more than 95% of radioactive nuclides exist in the liquid with particle sizes less than 0.1μm, while particle sizes greater than 0.45 μm account for less than 5%. After the sample was filtered by the demineralizer, the radioactive activity decreased. The flocculation precipitation in the waste liquid of the waste water recovery system has a certain contribution to the enrichment of nuclides. With the extension of time, the enrichment of transition elements such as cobalt and manganese is particularly obvious, so that it is distributed in the liquid again with a large particle size. In addition, large particle size substances such as colloids in seawater have a certain adsorption effect on radionuclides, which will lead to its aggregation effect again.
Uncovering the clinical relevance of unclassified variants in DNA repair genes: a focus on BRCA negative Tunisian cancer families
Authors: Boujemaa Maroua , Nouira Fatma , Jandoubi Nouha , Mejri Nesrine , Bouaziz Hanen , Charfeddine Cherine , Ben Nasr Sonia , Labidi Soumaya , El Benna Houda , Berrazega Yosra , Rachdi Haifa , Daoud Nouha , Benna Farouk , Haddaoui Abderrazek , Abdelhak Sonia , Samir Boubaker Mohamed , Boussen Hamouda , Hamdi Yosr 
Published: January 2024

Introduction: Recent advances in sequencing technologies have significantly increased our capability to acquire large amounts of genetic data. However, the clinical relevance of the generated data continues to be challenging particularly with the identification of Variants of Uncertain Significance (VUSs) whose pathogenicity remains unclear. In the current report, we aim to evaluate the clinical relevance and the pathogenicity of VUSs in DNA repair genes among Tunisian breast cancer families.Methods: A total of 67 unsolved breast cancer cases have been investigated. The pathogenicity of VUSs identified within 26 DNA repair genes was assessed using different in silico prediction tools including SIFT, PolyPhen2, Align-GVGD and VarSEAK. Effects on the 3D structure were evaluated using the stability predictor DynaMut and molecular dynamics simulation with NAMD. Family segregation analysis was also performed.Results: Among a total of 37 VUSs identified, 11 variants are likely deleterious affecting ATM, BLM, CHEK2, ERCC3, FANCC, FANCG, MSH2, PMS2 and RAD50 genes. The BLM variant, c.3254dupT, is novel and seems to be associated with increased risk of breast, endometrial and colon cancer. Moreover, c.6115G&gt;A in ATM and c.592+3A&gt;T in CHEK2 were of keen interest identified in families with multiple breast cancer cases and their familial cosegregation with disease has been also confirmed. In addition, functional in silico analyses revealed that the ATM variant may lead to protein immobilization and rigidification thus decreasing its activity. We have also shown that FANCC and FANCG variants may lead to protein destabilization and alteration of the structure compactness which may affect FANCC and FANCG protein activity.Conclusion: Our findings revealed that VUSs in DNA repair genes might be associated with increased cancer risk and highlight the need for variant reclassification for better disease management. This will help to improve the genetic diagnosis and therapeutic strategies of cancer patients not only in Tunisia but also in neighboring countries.
A mendelian randomization study investigates the causal relationship between immune cell phenotypes and cerebral aneurysm
Authors: Shi Xingjie , Wang Tao , Teng Da , Hou Shiqiang , Lin Ning 
Published: January 2024

Background: Cerebral aneurysms (CAs) are a significant cerebrovascular ailment with a multifaceted etiology influenced by various factors including heredity and environment. This study aimed to explore the possible link between different types of immune cells and the occurrence of CAs.Methods: We analyzed the connection between 731 immune cell signatures and the risk of CAs by using publicly available genetic data. The analysis included four immune features, specifically median brightness levels (MBL), proportionate cell (PC), definite cell (DC), and morphological attributes (MA). Mendelian randomization (MR) analysis was conducted using the instrumental variables (IVs) derived from the genetic variation linked to CAs.Results: After multiple test adjustment based on the FDR method, the inverse variance weighted (IVW) method revealed that 3 immune cell phenotypes were linked to the risk of CAs. These included CD45 on HLA DR+NK (odds ratio (OR), 1.116; 95% confidence interval (CI), 1.001–1.244; p = 0.0489), CX3CR1 on CD14− CD16− (OR, 0.973; 95% CI, 0.948–0.999; p = 0.0447). An immune cell phenotype CD16− CD56 on NK was found to have a significant association with the risk of CAs in reverse MR study (OR, 0.950; 95% CI, 0.911–0.990; p = 0.0156).Conclusion: Our investigation has yielded findings that support a substantial genetic link between immune cells and CAs, thereby suggesting possible implications for future clinical interventions.
Modern contraceptive prevalence and its predictors among non-refugee and refugee Somali women in Nairobi city, Kenya; a comparative view
Authors: Gitonga Eliphas , Gage Anastasia J. 
Published: January 2024

Introduction and methodsThis study sought to determine the prevalence and predictors of modern contraceptive use among non-refugee and refugee Somali women in Nairobi City, Kenya. The analysis was based on 976 currently married Somali women aged 15–39 years (non-refugees; 523, refugees; 415) who were interviewed in a 2021 household survey conducted in Kamukunji, Embakasi, and Ruaraka sub-counties of Nairobi City. The analysis was stratified by refugee status and multivariable logistic regression were run to determine predictors of modern contraceptive use in each group.ResultsThe prevalence of modern contraceptives was 34% for the total sample and 43% and 24% for non-refugees and refugees, respectively. The main methods of contraception among non-refugees were injectables, implants, and daily pills, while refugees mainly used male condoms, implants, and injectables. Stratified multivariable analysis showed that residence in formal vs. informal settlements was associated with significantly higher odds of modern contraceptive use among non-refugees but significantly lower odds among refugees, after controlling for other factors. Interaction terms confirmed that the strength of the associations of these variables with the odds of modern contraceptive use varied significantly by refugee status.ConclusionUse of modern methods of contraception was lower among non-refugee and refugee Somali women compared to the national average and refugee status moderated the association of some predictor variables with the odds of modern contraceptive use. To increase use of modern contraceptives in urban areas, it is recommended that the Ministry of Health, refugee agencies, and county governments engage with the Somali community and implement appropriate interventions to empower refugee women economically and promote their access to and use of voluntary contraception services as soon as they settle in urban areas.
Molecular characterization of the PhiKo endolysin from Thermus thermophilus HB27 bacteriophage phiKo and its cryptic lytic peptide RAP-29
Authors: Szadkowska Monika , Kocot Aleksandra Maria , Sowik Daria , Wyrzykowski Dariusz , Jankowska Elzbieta , Kozlowski Lukasz Pawel , Makowska Joanna , Plotka Magdalena 
Published: January 2024

IntroductionIn the era of increasing bacterial resistance to antibiotics, new bactericidal substances are sought, and lysins derived from extremophilic organisms have the undoubted advantage of being stable under harsh environmental conditions. The PhiKo endolysin is derived from the phiKo bacteriophage infecting Gram-negative extremophilic bacterium Thermus thermophilus HB27. This enzyme shows similarity to two previously investigated thermostable type-2 amidases, the Ts2631 and Ph2119 from Thermus scotoductus bacteriophages, that revealed high lytic activity not only against thermophiles but also against Gram-negative mesophilic bacteria. Therefore, antibacterial potential of the PhiKo endolysin was investigated in the study presented here.MethodsEnzyme activity was assessed using turbidity reduction assays (TRAs) and antibacterial tests. Differential scanning calorimetry was applied to evaluate protein stability. The Collection of Anti-Microbial Peptides (CAMP) and Antimicrobial Peptide Calculator and Predictor (APD3) were used to predict regions with antimicrobial potential in the PhiKo primary sequence. The minimum inhibitory concentration (MIC) of the RAP-29 synthetic peptide was determined against Gram-positive and Gram-negative selected strains, and mechanism of action was investigated with use of membrane potential sensitive fluorescent dye 3,3′-Dipropylthiacarbocyanine iodide (DiSC3(5)).Results and discussionThe PhiKo endolysin is highly thermostable with melting temperature of 91.70°C. However, despite its lytic effect against such extremophiles as: T. thermophilus, Thermus flavus, Thermus parvatiensis, Thermus scotoductus, and Deinococcus radiodurans, PhiKo showed moderate antibacterial activity against mesophiles. Consequently, its protein sequence was searched for regions with potential antibacterial activity. A highly positively charged region was identified and synthetized (PhiKo105-133). The novel RAP-29 peptide lysed mesophilic strains of staphylococci and Gram-negative bacteria, reducing the number of cells by 3.7–7.1 log units and reaching the minimum inhibitory concentration values in the range of 2–31 μM. This peptide is unstructured in an aqueous solution but forms an α-helix in the presence of detergents. Moreover, it binds lipoteichoic acid and lipopolysaccharide, and causes depolarization of bacterial membranes. The RAP-29 peptide is a promising candidate for combating bacterial pathogens. The existence of this cryptic peptide testifies to a much wider panel of antimicrobial peptides than thought previously.
Effects of water volume of drip irrigation on soil bacterial communities and its association with soil properties in jujube cultivation
Authors: Li Zhaoyang , Yang Yuhui , Liu Jiangfan , Jiang Wenge , Gao Yang 
Published: January 2024

IntroductionJujube is one of an important crop in Xinjiang, China, a area suffered by water scarcity and DI has been proven as a suitable mode for jujube cultivation. Soil bacterial community play a vital role in biogeochemical cycles to support the crop growth, and water content is considered as one of the important factors for them. However, limited research has explored the optimum irrigation strategies, such as water volume of DI, to maximize the benefits of jujube cultivation by regulating the soil bacterial communities.MethodsTherefore, in this study, we conducted DI experiments on jujube fields in Xinjiang with three different water volume levels, and measured the soil properties and bacterial communities of the flowering and fruit setting (FFS) and end of growth (EG) stages.Results and discussionSignificant lower jujube yield and soil available nutrients were observed in samples with low water amount. In addition, we discovered significant effects of the water amount of DI and jujube growth stages on soil bacterial communities. Based on the compare of samples among different growth stages and water amounts some growth stage related bacterial genera (Mycobacterium, Bradyrhizobium, and Bacillus) and water amount-related bacterial phyla (Chloroflexi, Nitrospirota, and Myxococcota) were recognized. Moreover, according to the results of null model, soil bacterial communities were governed by stochastic and deterministic processes under middle and low water volumes of DI, respectively. Finally, we deduced that middle water amount (600 mm) could be the optimal condition of DI for jujube cultivation because the higher jujube yield, deterministic assembly, and stronger correlations between soil properties and bacterial community under this condition. Our findings provide guidance for promoting the application of DI in jujube cultivation, and further research is needed to investigate the underlying mechanisms of soil bacterial community to promote the jujube yield.
The effect of a web-based lifestyle intervention on nutritional status and physical activity on prevention of COVID-19: a randomized controlled trial in women's empowerment
Authors: Pourfarzi Farhad , Kamran Aziz , Zare Maryam , Mohammadshahi Jafar 
Published: January 2024

BackgroundHealthy dietary intake and physical activity affect the immune systems. The present study aimed to investigate the effects of a web-based lifestyle intervention on nutritional status, physical activity, and prevention of COVID-19.MethodsThree hundred-three women (30–60 years old), who did not have COVID-19 in the City of Ardabil, participated in this study. Participants were randomized into an intervention (n = 152) or control group (n = 151). The intervention group received eight online educational sessions focusing on a healthy diet and physical activity via the website. There was no educational session for the control group during the intervention, but they were placed on the waiting list to receive the intervention and given access to the website and educational content after the follow-up. Outcomes were nutritional status, physical activity, immunoglobulin G (IgG), and immunoglobulin M (Ig M) antibody titers against the virus. They were evaluated at the baseline, after 4 and 12 weeks.ResultsSignificant improvements in weight (P &lt; 0.001), BMI (P &lt; 0.001), total energy (P = 0.006), carbohydrate (P = 0.001), protein (P = 0.001), and fat (P &lt; 0.001) were found for the intervention group compared to the control group during the study. MET-min/week for moderate physical activity increased during the time for the intervention and control groups (P &lt; 0.001 and P = 0.007, respectively). MET-min/week for walking activity rose in the post-intervention and follow-up compared to that in the baseline in the groups (P &lt; 0.001 for both groups). Total physical activity was increased during the study (P &lt; 0.001) for both groups. The mean of serum IgG and IgM titers against the virus were increased during the study in both groups in time effect (P &lt; 0.001). There was a significant time x group interaction for carbohydrate and fat intakes (P = 0.005 and P = 0.004, respectively).ConclusionThe web-based lifestyle intervention may improve nutritional status and physical activity, and have the potential to reduce the risk of contracting a COVID-19 infection.
Case report: The first case of concurrent breast myeloid sarcoma and borderline phyllodes tumor with malignant features
Authors: Chun Li , Zeng Zhen , Guo Qianyu , He Yangjun 
Published: January 2024

BackgroundMyeloid sarcoma (MS) is a rare hematological malignancy characterized by the formation of a solid mass of myeloblasts outside the bone marrow, such as in the lymph nodes, skin, or bone. MS may arise de novo or concurrently with acute myeloid leukemia (AML), myeloproliferative neoplasm (MPN), or myelodysplastic syndrome (MDS). MS accounts for less than 1% of extramedullary acute myeloid leukemia cases. Phyllodes tumors (PTs) are a rare fibroepithelial breast tumor that can be benign, malignant, or borderline, and account for less than 1% of all breast cancers.Case presentationWe present a unique case of a 50-year-old woman with both breast MS and borderline PT with malignant features, which presented a diagnostic challenge. The patient initially presented with a mass in her right breast, and the initial fine-needle biopsy revealed the presence of immature myeloperoxidase (MPO)+ myeloid cells consistent with MS. Subsequent pathological analysis of tumor tissues after neoadjuvant radiotherapy and chemotherapy showed a borderline PT with malignant features. Following excision of the tumor, the patient experienced a local recurrence, which was also surgically removed. At 8 months post-surgery, the patient remains free of recurrence under close follow-up.ConclusionThis case highlights the importance of considering the possibility of concurrent malignancies in the differential diagnosis of complex breast masses and underscores the challenges involved in diagnosing and managing such cases. Additionally, we also emphasize the value of neoadjuvant radiotherapy and chemotherapy in MS.