bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between serum levels of soluble tumor necrosis factor receptors/CA 125 and disease progression in patients with epithelial ovarian malignancy
Authors: Burger Robert A. , Darcy Kathleen M. , DiSaia Philip J. , Monk Bradley J. , Grosen Elizabeth A. , Gatanaga Tetsuya , Granger Gale A. , Wang Jianmin , Tian Chunqiao , Hanjani Parviz , Cohn David E. 
Published: May 2004

AbstractBACKGROUNDA prospective study was undertaken within the Gynecologic Oncology Group to determine whether serum levels of soluble tumor necrosis factor receptors I (sTNFR‐I) and II (sTNFR‐II), alone or in combination with CA 125, were associated with clinicopathologic characteristics or outcome in patients with epithelial ovarian malignancies.METHODSQuantitative immunoassays were performed on valid pretreatment serum specimens obtained from patients with epithelial ovarian malignancies to assess levels of sTNFR‐I, sTNFR‐II, and CA 125. The authors then analyzed the results of these immunoassays for potential correlations with clinicopathologic characteristics and outcome.RESULTSThe median age of the 139 women evaluated was 59 years. Seventy‐eight percent had Stage III or IV disease, and 58% had serous carcinomas. sTNFR‐II was associated with age (P = 0.013), and CA 125 was associated with histologic subtype (P = 0.0009). In addition, sTNFR‐I (P = 0.037) and CA 125 (P < 0.0001) were associated with extent of disease. After adjusting for patient age, histologic subtype, and extent of disease, all three biomarkers were predictive of progression‐free survival, but not overall survival, when the combination was included in the model. The authors observed a 51% reduction (hazard ratio [HR], 0.49; 95% confidence interval [CI], 0.24–0.99), a 2.9‐fold increase (HR, 2.87; 95% CI, 1.15–7.20), and a 22% increase (HR, 1.22; 95% CI, 0.99–1.51) in the risk of progression for each unit increase in the log‐transformed levels of sTNFR‐I, sTNFR‐II, and CA 125, respectively.CONCLUSIONSThe observations made in the current study—that among patients with low or high CA 125 levels, those with high sTNFR‐I levels and low sTNFR‐II levels had the lowest risk, that patients with low‐low or high‐high sTNFR‐I and sTNFR‐II levels, respectively, had an intermediate risk, and that patients with low sTNFR‐I levels and high sTNFR‐II levels had the highest risk of progression—suggested the potential value of simultaneous assessment of all three biomarkers in patients with epithelial ovarian malignancies. Cancer 2004. © 2004 American Cancer Society.
Smoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma
Authors: Chang Hsiao Wen , Ling Guang Sheng , Wei William Ignace , Yuen Anthony Po‐Wing 
Published: May 2004

AbstractBACKGROUNDThe consumption of tobacco and alcohol has been implicated in the development of head and neck squamous cell carcinoma (HNSCC). Promoter methylation of tumor suppressor genes is common in HNSCC. In this study, the authors evaluated the effects of tobacco and alcohol on p15 gene methylation of cells in cells from the mouth and throat of physically healthy individuals and patients with HNSCC.METHODSThe study participants were divided into 3 groups, including a group of 37 healthy nonsmokers and nondrinkers, a group of 22 healthy smokers and/or drinkers and a group of 31 patients with HNSCC.RESULTSMethylation of p15 was detected in cells obtained from mouth and throat (M&T) rinsing fluid samples from 3 of 37 healthy individuals (8%) who did not drink or smoke, from 15 of 22 healthy smokers and/or drinkers (68%), and from 15 of 31 patients (48%) with HNSCC. Among 31 patients with HNSCC, 20 patients (65%) had methylated p15 gene in their tumor biopsies. With the use of β‐actin as a reference, the ratio of methylated p15 to β‐actin was calculated as an index of the percentage of cells with p15 methylation. The percentage of exfoliated cells from M&T rinsing fluid samples that had p15 methylation ranged from 0% to 11% for patients with HNSCC and from 0% to 21% for healthy smokers/drinkers, respectively. The methylation index of tumor cells with p15 methylation ranged from 0% to 65%.CONCLUSIONSThe results suggest that p15 gene methylation can be induced by chronic smoking and drinking and may play a role in the very early stages of carcinogenesis in HNSCC. Cancer 2004. © 2004 American Cancer Society.
Immunochemotherapy with rituximab and temozolomide for central nervous system lymphomas
Authors: Wong Eric T. , Tishler Roy , Barron Loretta , Wu Julian K. 
Published: May 2004

AbstractBACKGROUNDMethotrexate‐based and alkylator‐based chemotherapy regimens are associated with renal and bone marrow toxicities, which limit their use in patients with central nervous system (CNS) lymphomas. The authors report their experience with an immunochemotherapy regimen consisting of rituximab and temozolomide in patients with primary or metastatic CNS lymphoma.METHODSSeven patients who had received rituximab and temozolomide were identified from the database of the brain tumor clinic at the authors' institution: three patients had developed recurrent primary CNS lymphoma (PCNSL), one patient had newly diagnosed PCNSL but had poor renal function, and three other patients with systemic non‐Hodgkin lymphoma developed recurrent lymphoma in the brain only. Patients were scheduled to receive 4 cycles of induction rituximab on Day 1 and temozolomide on Days 1–5 of a 28‐day cycle. Thereafter, their treatment included a total of up to 8 maintenance cycles of temozolomide alone on Days 1–5 of a 28‐day cycle. A gadolinium‐enhanced magnetic resonance image of the head was obtained after every two cycles of treatment.RESULTSAll patients received rituximab without toxicity. Of the 4 patients who received induction temozolomide at doses > 150 mg/m2 daily on Days 1–5, 2 experienced Grade 2 leukopenia and thrombocytopenia. Five patients achieved a radiographic complete response, and two patients had partial responses after induction treatment. The median response duration was 6 months (range 3–12+ months), and the median survival was 8 months (range 3+–12+ months).CONCLUSIONSAlthough median survival was short, immunochemotherapy with rituximab and temozolomide was well tolerated and exhibited efficacy in this elderly and heavily pretreated cohort. The data obtained in the current study suggest that the optimal induction dose combination consists of rituximab 375 mg/m2 on Day 1 and temozolomide 150 mg/m2 daily on Days 1–5. Cancer 2004. © 2004 American Cancer Society.
Imatinib mesylate therapy reduces bone marrow fibrosis in patients with chronic myelogenous leukemia
Authors: Bueso‐Ramos Carlos E. , Cortes Jorge , Talpaz Moshe , O'Brien Susan , Giles Francis , Rios Mary Beth , Medeiros L. Jeffrey , Kantarjian Hagop 
Published: May 2004

AbstractBACKGROUNDReticulin‐stained bone marrow fibrosis is associated with a poor prognosis in patients with chronic myelogenous leukemia (CML). Resolution of fibrosis with therapy may improve patient outcome.METHODSThe effect of imatinib therapy on bone marrow fibrosis was evaluated in 40 patients with chronic‐phase CML who were treated after interferon‐α failure.RESULTSThirty‐one patients (78%) had severe (Grade 3 or 4) reticulin fibrosis prior to therapy. After imatinib therapy was administered for 3 to > 24 months, fibrosis was reduced by at least 2 grades in 19 of the 31 patients (61%) and by at least 1 grade in 34 patients (85%). There was no correlation noted between reduction of fibrosis and cytogenetic response. However, a reduction in fibrosis was found to correlate with a reduction in bone marrow megakaryocytosis (P = 0.002).CONCLUSIONSTreatment with imatinib mesylate appears to reduce CML‐associated bone marrow fibrosis in most patients who are treated during the chronic phase of disease. This effect may be independent of the degree of suppression of Philadelphia chromosome‐positive cells, and may improve prognosis in patients with CML. Cancer 2004. © 2004 American Cancer Society.
S‐cone connections of the diffuse bipolar cell type DB6 in macaque monkey retina
Authors: Lee Sammy C.S. , Jusuf Patricia R. , Grünert Ulrike 
Published: May 2004

AbstractPrevious studies of primate retinae have shown that diffuse bipolar (DB) cells contact all the cones in their dendritic field, suggesting there is no spectral selectivity in the functional input to DB cells. However, since short‐wavelength sensitive (S) cones make up less than 10% of the total cone population, specialized connectivity with S‐cones is difficult to detect. In the present study, the S‐cone connectivity of a subtype of DB cells, the DB6 cell, was studied in macaque monkey retina. Pieces of macaque retina were processed with antibodies to CD15 to stain DB6 cells and antibodies to the S‐cone opsin to identify S‐cones. Immunoreactivity was visualized using immunoperoxidase or immunofluorescence. Some preparations were additionally processed with peanut agglutinin coupled to fluorescein to reveal medium‐ and long‐wavelength sensitive (M/L) cones. The preparations were analyzed using conventional and deconvolution light microscopy. The majority of DB6 cells had one or two S‐cones in their dendritic field and the majority of S‐cones were located in the dendritic field of DB6 cells. On average, 80% of the S‐cones and 81% of the M/L cones contacted DB6 cells. The average number of dendritic terminals at cone pedicles did not differ between the cone types. However, the total number of DB6 dendritic terminals receiving input from M/L‐cone pedicles was about eight times higher than the total number of dendritic terminals at S‐cone pedicles. In conclusion, DB6 cells make indiscriminate contact with all cone types, but receive their major input from M/L‐cones and thus carry a “Yellow‐ON” spectral signal. J. Comp. Neurol. 474:353–363, 2004. © 2004 Wiley‐Liss, Inc.
Wood photography using light irradiation and heat treatment
Authors: Mitsui Katsuya , Murata Akihiro , Tsuchikawa Satoru , Kohara Mitsuhiro 
Published: May 2004

AbstractTo apply the coloring method using light irradiation and thermal treatment to print photographs on wood, the effect of the transmittance of negative films was investigated. ΔE* decreased with light irradiation when specimens covered with films with transmittances exceeding 20% were irradiated for 100 h. It was thought that this phenomenon was due to the decrease in Δb*. The color of light‐irradiated wood changed remarkably with thermal treatment; however, the change in the color of exposed specimens covered with films with transmittances exceeding roughly 20% became constant. Clear photographs could be printed on wood using negative films with transmittances less than approximately 20%. Furthermore, the difference between the maximum and minimum values of ΔE* after thermal treatment was about 22. Humans can distinguish four to seven colors that can be created by this method. © 2004 Wiley Periodicals, Inc. Col Res Appl, 29, 312–316, 2004; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/col.20027
Quantification of EGFP expression on Molt‐4 T cells using calibration standards
Authors: Gerena‐López Y. , Nolan J. , Wang L. , Gaigalas A. , Schwartz A. , Fernández‐Repollet E. 
Published: May 2004

AbstractBackgroundEnhanced green fluorescent protein (EGFP) is used extensively to assess gene expression on cells; however, quantification of this expression by flow cytometry has been limited by the unavailability of calibration standards. Thus, we characterized the response of an experimental set of EGFP calibration standards to environmental changes and then quantitate the expression of EGFP, in molecules of equivalent soluble fluorochrome (MESF) units, of a transfected Molt‐4 T cell line by flow cytometry.MethodsCharacterization of the EGFP standards: EGFP standards were equilibrated in suspension solutions having a pH range of 5.0–9.0, temperatures of 37–80°C, and osmolalities of 100–600 mOsm/kg. Quantification of EGFP on cells: For transfections, Molt‐4 T cells were incubated with two different concentrations (0.2 μg and 0.4 μg) of pEGFP‐N2 vector and the EGFP expression was quantified after 48 h by flow cytometry using the EGFP standards and by the cytofluor technique using a standard curve of known EGFP solutions.ResultsThe fluorescence intensity of the EGFP standards increased from pH 5.0 to 9.0 and remained relatively constant from 37°C to 65°C, and from 100 to 600 mOsm/kg. After transfection, the expression of the populations with high and low EGFP expression averaged 8,098 ± 584 MESF and 3,808 ± 375 MESF respectively. No significant differences were observed after comparing the MESF values obtained by flow cytometry and the values obtained by Cytofluor technique (high: 8,791 ± 492 MESF; low: 4,082 ± 398 MESF).ConclusionsOur data demonstrate the feasibility of using calibration standards to quantify EGFP expression on cells. Our results emphasize the importance of monitoring the effects of environmental changes in the fluorescence intensity of both standards and samples when quantifying the expression of EGFP on living cells. © 2004 Wiley‐Liss, Inc.
Recent Advances in the Mitsunobu Reaction: Modified Reagents and the Quest for Chromatography‐Free Separation
Authors: Dembinski Roman 
Published: May 2004

AbstractRecent progress in synthetic approaches to the Mitsunobu reaction are reviewed. Efforts that have focused on the phase‐switching modifications of phosphane, azodicarboxylate, and nucleophilic components are emphasized. An approach to product separation with fluorous compounds is reviewed in detail. Alternative reagents and recently introduced products are cited. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Volatile constituents of satsuma mandarins growing in Korea
Authors: Choi Hyang‐Sook 
Published: May 2004

AbstractThe volatile ﬂavour components from cold‐pressed peel oils (CPOs) of three varieties of Korean satsuma mandarin (Citrus unshiu Marcov. var. Miyamoto; C. unshiu Marcov. var. Miyagawa‐wase; and C. unshiu Marcov. var. Aoshima) were analysed by GC, GC–MS and GC–olfactometry (GC–O). One hundred and sixteen volatile components were identiﬁed in this study by instrumental analyses, and 51 components were detected by snifﬁng test using GC–O. Limonene (82.8–89.2%) was the most abundant component in these samples, followed by γ‐terpinene (4.2–5.1%) and myrcene (1.7%). β‐Caryophyllene was the principal sesquiterpene hydrocarbon, its content in C. unshiu Marcov. var. Miyamoto being higher than in other varieties. The CPOs of the three satsuma mandarin varieties can easily be distinguished by the percentages of aldehydes, alcohols, ketones, esters and oxides. The sweet and fruity ﬂavour was stronger in C. unshiu Marcov. var. Miyamoto, while the herbaceous ﬂavour was stronger in C. unshiu Marcov. var. Miygawa‐wase and var. Aoshima. Copyright © 2004 John Wiley & Sons, Ltd.
Epoxidation–cyclization of rosewood oxides
Authors: Bombarda Isabelle , Cezanne Lionel , Gaydou Emile M. 
Published: May 2004

AbstractThe epoxidation reaction of linalool by MCPBA led to two furanoid oxides (35.7 and 37.0%), known as rosewood oxides, two pyranoid oxides (2.4 and 1.9%) and four rosewood epoxide derivatives. Reaction with BF3‐MeOH of the epoxidized cis‐furanoid oxide allowed the isolation of a new bicyclic compound [2‐(methoxymethyl)‐1,4,4‐trimethyl‐3,8‐dioxabicyclo(3.2.1)octane], whereas the epoxidized trans‐furanoid oxide led to a new monocyclic diol methoxy ether [2‐(1‐hydroxy‐2‐methoxyethyl)‐5‐(1‐hydroxy‐1‐methylethyl)‐2‐methyltetrahydrofuran]. 2D‐NMR analyses were used for structure determination. Copyright © 2004 John Wiley & Sons, Ltd.
Composition of seeds and stems essential oils of Pituranthos scoparius (Coss. & Dur.) Schinz
Authors: Vérité P. , Nacer A. , Kabouche Z. , Seguin E. 
Published: May 2004

AbstractThe composition of the essential oils of stems and seeds of Pituranthos scoparius was determined by GC–MS. Almost 50 compounds were identiﬁed. In the seed oil, the main compounds were α‐pinene (8.2%), β‐pinene (4.6%), p‐cymene (7.5%), limonene (11.2%), thymol (5.9%), myristicin (11.1%) and dill apiole (12.2%); in the stem oil, the main compounds were α‐pinene (6.8%), α‐phellandrene (7.1%), limonene (9.8%), methyl eugenol (5.6%) germacrene D (12.7%), myristicin (7.2%), spathulenol (4.5%) and β‐eudesmol (4.1%). Copyright © 2004 John Wiley & Sons, Ltd.
Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and ﬁxative properties of the essential oil of Rosa damascena L. from China
Authors: Jirovetz Leopold , Buchbauer Gerhard , Stoyanova Albena , Balinova Anastasia , Guangjiun Zhang , Xihan Ma 
Published: May 2004

AbstractThe composition of the essential oil and the SPME‐headspace volatiles of Rosa damascena L. (Rosaceae) from China were investigated by gas chromatographic–spectroscopic (GC–FID and GC–MS) and olfactoric methods to identify those compounds responsible for the characteristic, pleasant ﬂoral odour of these valuable, high‐priced ﬂower oils. Monoterpenes especially were found to be fragrance target compounds, while additionally some aliphatic hydrocarbons will have ﬁxative effects and therefore are responsible for a longer‐lasting odour impression (tested by olfactory evaluation after application to human skin) of this rose oil. The main compounds [concentration higher than 3.0%; calculated as %‐peak area of GC–FID analyses using a polar (carbowax) column] of the 71 totally identiﬁed constituents of the two rose samples are as follows: Chinese rose oil: citronellol (30.71%), nonadecane (16.95%), geraniol (16.11%), nerol (7.57%), heneicosane (7.04%) and 9‐eicosene (4.71%); and SPME‐headspace of the Chinese rose oil: citronellol (37.05%), geraniol (18.62%), nerol (9.91%) and methyl eugenol (3.56%). A correlation of the identiﬁed constituents of the two rose oil samples with their single odour impressions is also given and the signiﬁcant change of the composition of the SPME‐headspace samples after rose oil application on human skin discussed. Copyright © 2004 John Wiley & Sons, Ltd.
Composition and antimicrobial activity of Achillea clavennae and Achillea holosericea essential oils
Authors: Stojanović G. , Asakawa Y. , Palić R. , Radulović N. 
Published: May 2004

AbstractThe composition of the essential oils of Achillea clavennae and Achillea holosericea were analyzed by GC/MS. The main constituting compounds of the A. clavennae essential oil were camphor (41.9%) and 1,8‐cineole (22.5%), while the most abundant compounds in the A. holosericea oil were borneol (30.2%) and camphor (14.8%). The antibacterial activity of the oil of A. clavennae was tested for the ﬁrst time. Both examined oils showed strong activity against all tested microorganisms (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Aspergillus niger). Copyright © 2004 John Wiley & Sons, Ltd.
Expression of the low‐affinity neurotrophin receptor, p75NTR, is upregulated by oligodendroglial progenitors adjacent to the subventricular zone in response to demyelination
Authors: Petratos Steven , Gonzales Michael F. , Azari Michael F. , Marriott Mark , Minichiello Rebecca A. , Shipham Kylie A , Profyris Christos , Nicolaou Antonis , Boyle Kristy , Cheema Surindar S. , Kilpatrick Trevor J. 
Published: May 2004

AbstractPrecursor cells have the capacity to repopulate the demyelinated brain, but the molecular mechanisms that facilitate their recruitment are largely unknown. The low‐affinity neurotrophin receptor, p75NTR, may be one of these regulators; however, its expression profile by oligodendroglia within the multiple sclerosis (MS) brain remains uncertain. We therefore assessed the expression profile of this receptor within 8 MS and 4 control brains. We found no evidence of expression of p75NTR by mature oligodendrocytes. Instead, we demonstrated the presence of p75NTR on a subgroup of NG2‐positive oligodendroglial progenitors in a periventricular plaque in one MS sample. Notably, p75NTR‐expressing cells were also detected within the subventricular zone (SVZ) of this brain, adjacent to the periventricular plaque. In animals with experimental demyelination we observed similar patterns of p75NTR expression, initially confined to precursor cells within the SVZ, followed at later stages in the disease course by its expression amongst a subset of oligodendroglial progenitors within the corpus callosum. These data suggest that a population of precursor cells within the SVZ can be induced to express p75NTR and to subsequently assume an oligodendroglial progenitor phenotype in response to demyelination in the adjacent white matter. © 2004 Wiley‐Liss, Inc.
Evaluation of an image‐guided, robotically positioned transcranial magnetic stimulation system
Authors: Lancaster Jack L. , Narayana Shalini , Wenzel Dennis , Luckemeyer James , Roby John , Fox Peter 
Published: May 2004

AbstractThe emergence of transcranial magnetic stimulation (TMS) as a tool for investigating the brain has been remarkable over the past decade. While many centers are now using TMS, little has been done to automate the delivery of planned TMS stimulation for research and/or clinical use. We report on an image‐guided robotically positioned TMS system (irTMS) developed for this purpose. Stimulation sites are selected from functional images overlaid onto anatomical MR images, and the system calculates a treatment plan and robotically positions the TMS coil following that plan. A new theory, stating that cortical response to TMS is highest when the induced E‐field is oriented parallel to cortical columns, is used by the irTMS system for planning the position and orientation of the TMS coil. This automated approach to TMS planning and delivery provides a consistent and optimized method for TMS stimulation of cortical regions of the brain. We evaluated the positional accuracy and utility of the irTMS system with a B‐shaped TMS coil. Treatment plans were evaluated for sites widely distributed about a head phantom with well‐defined landmarks. The overall accuracy in positioning the planned site of the TMS coil was approximately 2 mm, similar to that reported for the robot alone. The estimated maximum range of error in planned vs. delivered E‐field strength was +4%, suggesting a high degree of accuracy and reproducibility in the planned use of the irTMS system. Hum. Brain Mapping 22:329–340, 2004. © 2004 Wiley‐Liss, Inc.
Distinct LIM domains of Hic‐5/ARA55 are required for nuclear matrix targeting and glucocorticoid receptor binding and coactivation
Authors: Guerrero‐Santoro Jennifer , Yang Lan , Stallcup Michael R. , DeFranco Donald B. 
Published: May 2004

AbstractHydrogen peroxide‐inducible clone‐5 (Hic‐5), belongs to the group III LIM domain protein family and contains four carboxyl‐terminal LIM domains (LIM1–LIM4). In addition to its role in focal adhesion signaling, Hic‐5 acts in the nucleus as a coactivator for some steroid hormone receptors such as the glucocorticoid receptor (GR) and androgen receptor (AR). Based upon its effect on AR transactivation, Hic‐5 has also been designated as ARA55. Here, we report mapping studies of Hic‐5/ARA55 functional domains and establish that LIM3 and LIM4 are necessary for maximal effects on GR transactivation. However, results from yeast two‐hybrid assays demonstrated that these two LIM domains together, while necessary, are not sufficient to interact with the tau2 transactivation domain of GR. LIM4 also functions as a nuclear matrix targeting sequence (NMTS) for Hic‐5/ARA55, as it is both necessary and sufficient to target a heterologous protein to the nuclear matrix. Thus, as suggested from previous analysis of LIM domain‐containing proteins, separate but highly related LIM domains serve distinct functions. © 2004 Wiley‐Liss, Inc.