bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring connectivity of resting-state EEG between BCI-literate and -illiterate groups
Authors: Park Hanjin , Jun Sung Chan 
Published: November 2023

AbstractAlthough Motor Imagery-based Brain-Computer Interface (MI-BCI) holds significant potential, its practical application faces challenges attributable to the phenomenon known as BCI-illiteracy. BCI researchers have attempted to predict BCI-illiteracy to mitigate this issue. As connectivity’s significance in neuroscience has grown, BCI researchers have applied connectivity to predict BCI-illiteracy with resting-state data. However, connectivity metrics’ use and interpretation of the results can be challenging for several reasons. Firstly, there are various connectivity metrics, each with its own advantages and disadvantages based on their underlying hypotheses and perspectives. These pros and cons are shaped by several factors, increasing the complexity of their application and interpretation. Secondly, it is unclear whether they are as acceptable as their developers have claimed. Thirdly, it is not evident which factor may influence the estimation of connectivity and which metric is suitable for this research. Therefore, this study conducted an empirical test to provide BCI researchers with a better understanding of connectivity. We analyzed three large public datasets using three functional connectivity (FC) and three effective connectivity (EC) metrics. Additionally, the structural difference in the resting-state network between BCI-literate and illiterate groups was examined. Our analysis revealed that the appropriate frequency range to measure connectivity varies depending upon the metric used. The alpha range was found to be suitable for FC, while the alpha, alpha + theta, and beta ranges were found to be appropriate for EC. Further, the results of estimating connectivity varied depending upon the dataset and metric used. Although we observed that BCI-literacy had stronger connections between nodes, no other significant structural differences were found between the two groups. However, BCI-literacy’s resting-state network displayed higher network efficiency compared to BCI-illiteracy, regardless of the metrics and dataset used. Therefore, it seems reasonable to use resting-state connectivity to predict BCI-illiteracy. Our conclusion is that each metric has its own specific hypothesis and perspective to measure connectivity under certain conditions.
H2A.Z chaperones converge on histone H4 acetylation for melanoma cell proliferation
Authors: Jostes Sina , Vardabasso Chiara , Dong Joanna , Carcamo Saul , Singh Rajendra , Phelps Robert , Meadows Austin , Hasson Dan , Bernstein Emily 
Published: November 2023

ABSTRACTHigh levels of H2A.Z promote melanoma cell proliferation and correlate with poor prognosis. However, the role of the two distinct H2A.Z histone chaperone complexes, SRCAP and P400-TIP60, in melanoma remains unclear. Here, we show that individual depletion ofSRCAP,P400, andVPS72(YL1)not only results in loss of H2A.Z deposition into chromatin, but also a striking reduction of H4 acetylation in melanoma cells. This loss of H4 acetylation is found at the promoters of cell cycle genes directly bound by H2A.Z and its chaperones, suggesting a highly coordinated regulation between H2A.Z deposition and H4 acetylation to promote their expression. Knockdown of each of the three subunits downregulates E2F1 and its targets, resulting in a cell cycle arrest akin to H2A.Z depletion. However, unlike H2A.Z deficiency, loss of the shared H2A.Z chaperone subunit YL1 induces apoptosis. Furthermore, YL1 is overexpressed in melanoma tissues, and its upregulation is associated with poor patient outcome. Together, these findings provide a rationale for future targeting of H2A.Z chaperones as an epigenetic strategy for melanoma treatment.
The Myc-Like Mlx Network Impacts Aging and Metabolism
Authors: Wang Huabo , Stevens Taylor , Lu Jie , Roberts Alexander , Land Clinton Van’t , Muzumdar Radhika , Gong Zhenwei , Vockley Jerry , Prochownik Edward V. 
Published: November 2023

AbstractThe “Mlx” and “Myc” Networks share many common gene targets. Just as Myc’s activity depends upon its heterodimerization with Max, the Mlx Network requires that the Max-like factor Mlx associate with the Myc-like factors MondoA or ChREBP. We show here that body-wideMlxinactivation, like that ofMyc,accelerates numerous aging-related phenotypes pertaining to body habitus and metabolism. The deregulation of numerous aging-related Myc target gene sets is also accelerated. Among other functions, these gene sets often regulate ribosomal and mitochondrial structure and function, genomic stability and aging. Whereas “MycKO” mice have an extended lifespan because of a lower cancer incidence, “MlxKO” mice have normal lifespans and a somewhat higher cancer incidence. Like Myc, Mlx, MondoA and ChREBP expression and that of their target genes, deteriorate with age in both mice and humans, underscoring the importance of life-long and balanced cross-talk between the two Networks to maintain normal aging.TeaserInactivation of the Myc-like “Mlx Network” in mice leads to phenotypic and molecular signs of premature aging and a cancer predisposition.
Inbred rat heredity and sex affect oral oxycodone self-administration and augmented intake in long sessions: correlations with anxiety and novelty-seeking
Authors: Sharp Burt M , Leng Shuangying , Huang Jun , Jones Caroline , Chen Hao 
Published: November 2023

AbstractOxycodone abuse begins with prescription oral oxycodone, yet vulnerability factors determining abuse are largely undefined. We evaluated genetic vulnerability in a rat model of oral oxycodone self-administration (SA): increasing oxycodone concentration/session (0.025-0.1mg/ml; 1,4,16-h) followed by extinction and reinstatement. Active licks and oxycodone intake were greater in females than males during 4-h and 16-h sessions (p< 0.001). Each sex increased intake during 16-h vs 4-h sessions (p<2e-16), but a subset of strains dramatically augmented intake at 16-h (p=0.0005). Heritability (h2) of active licks/4-h at increasing oxycodone dose ranged from 0.30-0.53. Under a progressive ratio schedule, breakpoints were strain-dependent (p<2e-16). Cued reinstatement was greater in females (p<0.001). Naive rats were assessed by elevated plus maze (EPM), open field (OF) and novel object interaction (NOI). We correlated these behaviors with 28 parameters of oxycodone SA. Anxiety-defining EPM traits were most associated with SA in both sexes, whereas more OF and NOI traits were SA-associated in males. Sex and heredity are major determinants of motivation to take and seek oxycodone; intake augments dramatically during extended access in specific strains; and pleiotropic genes affect anxiety and multiple SA parameters.
Different Diets Modulate the Gut Microbiome Compositions and Promote the Health ofApis mellifera
Authors: Kim Hyun Jee , Maigoro Abdulkadir Yusif , Lee Jeong Hyeon , Frunze Olga , Bilal Mustafa , Kwon Hyung Wook 
Published: November 2023

AbstractHoney bee (Apis mellifera) health is crucial for honey bee products, and effective pollination and is closely associated with gut bacteria. Various factors such as reduced habitat, temperature, disease, and diet affect the health of honey bees, by disturbing the homeostasis of the gut microbiota. In this study, high-throughput 16S rRNA gene sequencing was used to analyze the gut microbiota ofApis melliferasubjected to seven different diets. The identified microbiota in theApis melliferagut from all the diets consisted ofLactobacillus(62%), followed byRhizobiaceae(21%),Snodgrassella(4%), andErwiniaceae(4%) among other 33 genera. Based on diet types,Lactobacillusa lactic acid bacteria (LAB), dominates the microbiota with the highest relative abundance in AIGT+SAC (91%), AIGT+Soytide (88%), and AIGT+Apple juice (69%) diet groups.BifidobacteriumandCommensalibacterappeared as the second most abundant genera in AIGT+SAC and AIGT+Soytide diet groups, respectively. These bacteria are important markers for honey bee health. Considering the importance of these diets in shaping their host microbiome into a healthy status. Individual honey bee health (IHH) was observed to validate the quality and correlation between the microbiota and honey bee health. The results were consistent, indicating thatApis melliferafed on AIGT+Soytide and AIGT+SAC diet showed the highest health expression level of vitellogenin. The group with 60%Syrup possessingRhizobiaceaeas the dominant taxa showed poor health status. This finding paved the way for establishing a link between gut microbiota and IHH under different diets.
Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem function
Authors: Jing Xiaoyan , Gong Yanhai , Diao Zhidian , Ma Yan , Meng Yu , Chen Jie , Ren Yishang , Li Yinchao , Sun Weihan , Zhang Jia , Ji Yuetong , Liang Yuting , Cong Zhiqi , Li Shengying , Ma Bo , Cui Zhisong , Ma Li , Xu Jian 
Published: November 2023

AbstractAlthough microbiome-wide association studies (MWAS) have uncovered many marker organisms for an ecosystem trait, mechanisms of most microbiota-mediated processes remain elusive, due to challenges in validating the markers’in situmetabolic activities and tracing such activities to individual genomes. Here we introduced a phylogeny-metabolism dual-directed single-cell genomics approach called Fluorescence-In-Situ-Hybridization-guided Single-Cell Raman-activated Sorting and Sequencing (FISH-scRACS-Seq). It directly localizes individual cells from target taxon via a FISH probe for marker organism, profiles theirin situmetabolic functions via single-cell Raman spectra, sorts cells of target taxonomy and target metabolism, and produces indexed, high-coverage and precisely-one-cell genomes. From cyclohexane-contaminated seawater, cells representing the MWAS-derived marker taxon of γ-Proteobacteria and that are actively degrading cyclohexanein situwere directly identified via FISH and Raman respectively, then sorted and sequenced for one-cell full genomes. In such aPseudoalteromonas fuligineacell, we discovered a three-component cytochrome P450 system that can convert cyclohexane to cyclohexanolin vitro, representing a previously unknown group of cyclohexane-degrading enzymes and organisms. By culture-independently unveiling enzymes, pathways, genomes and theirin situfunctions specifically for those single-cells with ecological relevance, FISH-scRACS-Seq is a rational and generally applicable approach for dissecting and mining microbiota functions.TeaserFISH-scRACS-Seq is a new strategy to dissect microbiota functional mechanism at single-cell resolution.
Menopausal transition alters female skeletal muscle transcriptome
Authors: Juppi Hanna-Kaarina , Korhonen Tia-Marje , Sievänen Tero , Kovanen Vuokko , Kujala Urho M. , Aukee Pauliina , Cronin Neil , Sipilä Sarianna , Karvinen Sira , Laakkonen Eija K. 
Published: November 2023

AbstractMenopause is associated with unfavorable changes in body composition. Skeletal muscle cells are targets of hormonal regulation and are affected by the interplay between coding and non-coding RNAs. Muscle transcriptome, including messenger-RNA (mRNA), long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) has not previously been studied in women during the menopausal transition. Thus, we took a multi-RNA omics approach to get insight into transcriptome-wide events of menopause. Our study included baseline and follow-up muscle samples from seven early (EarlyMT) and 17 late perimenopausal (LateMT) women transitioning to early postmenopause during the study. Total RNA was sequenced and differential expression (DE) of the transcriptome was investigated. The potential gene functions were investigated with pathway analyses and protein level expression with Western Blot. We found 30 DE mRNA genes in EarlyMT and 19 in LateMT participating in pathways controlling cell death, growth, and interactions with external environment. Lack of protein level changes may indicate a specific role of the regulatory RNAs during menopause. Ten DE lncRNA transcripts were identified but did not result in DE lncRNAs genes. No DE miRNAs were found. Despite the lack of DE findings in regulatory RNAs, we identified putative regulatory networks, likely to be affected by estradiol availability. The changes in gene expression were correlated with observed changes in body composition variables, indicating muscularity and adiposity regulators to be affected by menopausal transition. In essence, the observed DE genes and their regulatory networks may offer novel mechanistic insights on factors affecting body composition during and after menopause.
Machine learning-guided optimization of p-coumaric acid production in yeast
Authors: Moreno-Paz Sara , van der Hoek Rianne , Eliana Elif , Zwartjens Priscilla , Gosiewska Silvia , dos Santos Vitor A.P. Martins , Schmitz Joep , Suárez-Diez Maria 
Published: November 2023

AbstractIndustrial biotechnology uses Design-Build-Test-Learn (DBTL) cycles to accelerate the development of microbial cell factories, required for the transition to a bio-based economy. To use them effectively, appropriate connections between each phase of the cycle are crucial. Using p-coumaric acid production inSaccharomyces cerevisieaas case study, we propose the use of one-pot library generation, random screening, targeted sequencing and machine learning (ML) as links during DBTL cycles. We showed that the robustness and flexibility of ML models strongly enable pathway optimization, and propose feature importance and SHAP values as a guide to expand the design space of original libraries. This approach led to a 68% increased production of p-coumaric acid within two DBTL cycles.
Business model innovation in sustainable entrepreneurship: co-evolutionary evidence from small accommodation firms
Authors: Baiocco Silvia , Paniccia Paola M.A. 
Published: November 2023

PurposeThis paper aims to better understand how business model innovation (BMI) occurs in the context of sustainable entrepreneurship, emphasizing the dialectical nature of entrepreneurial relationships. To do so, key interdependencies and reciprocal influences between internal/firm-specific and external/environmental factors underlying BMI for sustainability are analysed through co-evolutionary lenses.Design/methodology/approachA co-evolutionary framework is developed and applied to a longitudinal business model (BM) analysis of 15 Italian widespread hotels, which creatively use historic villages at risk of abandonment to establish their hotels.FindingsLargely influenced by the interplay between internal and external factors, BMI of widespread hotels occurs through multilevel co-adaptations, which are recognised as virtuous by all stakeholders involved. Effective variations of the BM value elements are selected resulting in circular economy practices, which are retained for successful BMI, radical (first) and incremental (thereafter). Knowledge of specific local and multi-local conditions, time awareness and a future-oriented temporal perspective, by both entrepreneurs and policymakers, favour this dynamic.Practical implicationsDeveloping targeted policies and practices based on increased organisational knowledge supported by indicators can help in selecting and retaining successful variations of BMs appropriately in/with time with positive effects on firms' performance and sustainable development.Originality/valueThis study provides a novel co-evolutionary framework that explicitly links sustainable entrepreneurship and BM concepts in the accommodation sector. It further proposes a dynamic and holistic explanation of BMI for sustainability from which the crucial roles of the time-knowledge binomial and circular practices emerge.
HelpUsGreen LLP: driving next-level growth by building a sustainable circular economy
Authors: Agarwal Richa , Ghura Amarpreet Singh , Narang Tanu 
Published: November 2023

Learning outcomes
On completion of this case study, students will be able to describe a circular economy and cross-marketing; discuss the common strategies under the circular economy paradigm; describe the benefits and risks of cross-marketing brand alliances and their strategic implications; examine the role of growth strategy in driving the growth of an organization; classify what constitutes the elements of a strategy for HelpUsGreen LLP; and discuss measures to be considered while selecting a partner for a cross-marketing brand alliance.

Case overview/synopsis
HelpUsGreen LLP was a start-up founded by Karan Rastogi in 2019, which manufactured incense sticks using the circular economy model. After deflecting from his earlier partner in 2019 and after approximately two years of effort, Rastogi expanded his business of making incense sticks using a circular economy model. Students through this case can step into the shoes of Rastogi, who, as of 9 January 2023, was in Kanpur and was in an urgent need to replicate a similar circular economy model in different cities. With over 21,060 tonnes of temple flower waste recycled, 210 tonnes of chemical pesticides offset and 100% natural products delivered, multiple opportunities emerged at the cross-marketing brand alliance and new product segment levels. Rastogi entered into several cross-marketing brand alliance arrangements and tasted success. Rastogi believed that choosing the right partner for cross-marketing was key to successful cooperation. Considering cross-marketing as a way forward, he urgently needed to develop a strategy that aligned with his mission to take HelpUsGreen LLP’s circular economy model to other parts of the country. Students should take into consideration the data regarding the processes at HelpUsGreen LLP that helped Rastogi restart from scratch and make assumptions to decide the growth route for entering different cities.

Complexity academic level
This case can be used as an introductory case in a post-graduate class on growth strategy for a business based on the circular economy model, as it delineates the challenges faced by a firm while creating a circular economy and managing its growth phase. The case can also be used in an entrepreneurship management course and a strategic management course. This case allows students to learn about the circular economy and challenges faced by the company during the growth phase. Thus, the case can be used for covering multiple perspectives related to growth strategy (e.g. the application of Ansoff matrix), for defining what is cross-marketing brand alliance and discussing what measures need to be considered while selecting a partner for cross-marketing brand alliance, and it is ideal for teaching the elements of strategy.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 11: Strategy.
The role of self-efficacy and psychological empowerment in explaining the relationship between emotional intelligence and work engagement
Authors: Hameli Kujtim , Ukaj Lekë , Çollaku Lum 
Published: November 2023

PurposeThis study aims to investigate the impact of emotional intelligence on work engagement by exploring the mediating roles of self-efficacy and psychological empowerment in this relationship.Design/methodology/approachUsing the survey method, data were collected through both physical and online means. The final sample comprised 304 teachers working in elementary, middle and high schools. Structural equation modeling (SEM) analysis, utilizing IBM Amos version 26, was employed to assess the hypothesized model.FindingsThe study's results revealed that emotional intelligence does not exert a direct impact on work engagement. Additionally, self-efficacy was not found to mediate the relationship between emotional intelligence and work engagement. However, psychological empowerment emerged as a mediating factor in this context. Furthermore, self-efficacy and psychological empowerment concurrently demonstrated significant mediation of the relationship between emotional intelligence and work engagement.Research limitations/implicationsThe findings underscore the significance of teachers' emotional intelligence, suggesting that it can have profound implications for their work engagement through the mediating mechanisms of self-efficacy and psychological empowerment. Educational principals and administrators are encouraged to prioritize the enhancement of teachers' psychological empowerment, recognizing it as a pivotal link between teachers' emotional intelligence and work engagement.Originality/valueThis study contributes to the field by comprehensively examining the mediating roles of self-efficacy and psychological empowerment, addressing prior empirical gaps, and enriching the understanding of how emotional intelligence influences work engagement within the educational sphere.
University students' perceptions of graduate employer selection tests
Authors: Quigley Martyn , Smith Charlotte , Stocker Eloise , Bradley Alexander 
Published: November 2023

PurposeThe purpose of the current study was to examine university students' knowledge, confidence and experience of popular graduate employer selection tests.Design/methodology/approachA cross-sectional self-report survey was administered to gather a sufficient number of quantitative responses from undergraduate students. A total of 241 students completed the survey with most of them being psychology students from Swansea University. Four key variables were examined: (1) students' experience, (2) confidence and (3) knowledge of selection tests and (4) their desire for more information about selection tests as part of their degree. An audit of selection tests used by the Times Top 100 graduate employers was also conducted.FindingsStudents tended to misjudge how often selection tests were used by employers, and generally lacked experience with these tests. Students' confidence in completing each test varied as a function of the selection test; however, prior experience with these tests positively predicted confidence. Additionally, over 70% of students reported a desire for further information about selection tests as part of their degree.Practical implicationsThese novel findings suggest that students could benefit from further information about selection tests as part of their degree programme which would be of benefit to both students and universities.Originality/valueThese findings are, to the authors knowledge, the first to explicitly assess second- and third-year undergraduate students' knowledge, experience and confidence with popular graduate employer selection tests and demonstrate that students would like more information about these tests on their programme.
Prioritising sustainable garment choice among high-volume fashion consumers
Authors: McNeill Lisa S. , Potdar Balkrushna , McQueen Rachel H. 
Published: November 2023

PurposeThe purpose of this paper is addressing the negative environment and social impacts of the fashion industry that has emerged as a major societal challenge in the last century; however, people continue to over-consume and over-waste textile products in the form of fashion garments. More research is required to understand how fashion consumption connects with perceptions of sustainability and sustainable action by individual consumers.Design/methodology/approachThis paper surveys 501 Australian and New Zealand consumers, on their fashion purchasing habits, and the role of sustainability in their fashion consumption behaviour.FindingsThe study found a relationship between higher levels of garment consumption and increased focus on sustainability in fashion choices. This finding is significant, as it suggests that fashion over-consumers are still mindful of the sustainable impacts of their consumption, and are motivated towards reducing that impact, despite not reducing their volume of consumption.Originality/valueThe study indicates the importance of a continued focus on transparency and traceability of fashion products in Australasia, as some over-consumers use this information to make better choices in the purchase situation, as opposed to acting on a general call to reduce consumption.