bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain glucose concentrations in patients with type 1 diabetes and hypoglycemia unawareness
Authors: Criego Amy B. , Tkac Ivan , Kumar Anjali , Thomas William , Gruetter Rolf , Seaquist Elizabeth R. 
Published: December 2004

AbstractAlthough it is well established that recurrent hypoglycemia leads to hypoglycemia unawareness, the mechanisms responsible for this are unknown. One hypothesis is that recurrent hypoglycemia alters brain glucose transport or metabolism. We measured steady‐state brain glucose concentrations during a glucose clamp to determine whether subjects with type 1 diabetes and hypoglycemia unawareness may have altered cerebral glucose transport or metabolism after exposure to recurrent hypoglycemia. We compared 14 subjects with diabetes and hypoglycemia unawareness to 27 healthy control subjects. Brain glucose concentrations were measured under similar metabolic conditions using in vivo 1H nuclear magnetic resonance (NMR) spectroscopy at 4 Tesla during a hyperglycemic clamp (plasma glucose = 16.7 mmol/l) with somatostatin and insulin. Subjects with type 1 diabetes and hypoglycemia unawareness had significantly higher brain glucose concentrations compared to that in controls under the same conditions (5.5 ± 0.3 vs. 4.7 ± 0.1 μmol/g wet weight, P = 0.016). These data suggest that changes in brain glucose transport or metabolism may occur as a result of recurrent hypoglycemia. © 2004 Wiley‐Liss, Inc.
Astrocyte glycogen metabolism is required for neural activity during aglycemia or intense stimulation in mouse white matter
Authors: Brown Angus M. , Sickmann Helle M. , Fosgerau Keld , Lund Trine M. , Schousboe Arne , Waagepetersen Helle S. , Ransom Bruce R. 
Published: December 2004

AbstractWe tested the hypothesis that inhibiting glycogen degradation accelerates compound action potential (CAP) failure in mouse optic nerve (MON) during aglycemia or high‐intensity stimulation. Axon function was assessed as the evoked CAP, and glycogen content was measured biochemically. Isofagomine, a novel inhibitor of central nervous system (CNS) glycogen phosphorylase, significantly increased glycogen content under normoglycemic conditions. When MONs were bathed in artificial cerebrospinal fluid (aCSF) containing 10 mM glucose, the CAP failed 16 min after exposure to glucose‐free aCSF. MONs bathed in aCSF plus isofagomine displayed accelerated CAP failure on glucose removal. Similar results were obtained in MONs bathed in 30 mM glucose, which increased baseline glycogen concentration. The ability of isofagomine to increase glycogen content thus was not translated into delayed CAP failure. This is likely due to the inability of the tissue to metabolize glycogen in the presence of isofagomine, highlighting the importance of glycogen in sustaining neural function during aglycemia. The hypothesis that glycogen breakdown supports intense neural activity was tested by blocking glycogen breakdown during periods of high‐frequency stimulation. The CAP area declined more rapidly when glycogen metabolism was inhibited by isofagomine, explicitly showing an important physiological role for glycogen metabolism during neural activity. © 2004 Wiley‐Liss, Inc.
Aryl acrylate based high‐internal‐phase emulsions as precursors for reactive monolithic polymer supports
Authors: Krajnc Peter , Štefanec Dejan , Brown Jane F. , Cameron Neil R. 
Published: December 2004

AbstractWater‐in‐oil high‐internal‐phase emulsions (HIPEs), containing 4‐nitrophenyl acrylate and 2,4,6‐trichlorophenyl acrylate as reactive monomers, were prepared and polymerized, and highly porous monolithic materials resulted. The novel materials were studied by combustion analysis, Fourier transform infrared spectroscopy scanning electron microscopy, mercury porosimetry, and N2 adsorption/desorption analysis. With both esters, cellular macroporous monolithic polymers were obtained; the use of 4‐nitrophenyl acrylate resulted in a cellular material with void diameters between 3 and 7 μm and approximately 3‐μm interconnects, whereas the use of 2,4,6‐trichlorophenyl acrylate yielded a foam with void diameters between 2 and 5 μm, most interconnects being around 1 μm. The resulting monoliths proved to be very reactive toward nucleophiles, and possibilities of functionalizing the novel polymer supports were demonstrated via reactions with amines bearing additional functional groups and via the synthesis of an acid chloride derivative. Tris(hydroxymethyl)aminomethane and tris(2‐aminoethyl)amine derivatives were obtained. The hydrolysis of 4‐nitrophenylacrylate removed the nitrophenyl group, yielding a monolithic acrylic acid polymer. Furthermore, functionalization to immobilized acid chloride was performed very efficiently, with more than 95% of the acid groups reacting. The measurement of the nitrogen content in 4‐nitrophenyl acrylate poly(HIPE)s after various times of hydrolysis showed the influence of the total pore volume of the monolithic polymers on the velocity of the reaction, which was faster with the more porous polymer. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 296–303, 2005
Chemical synthesis of biodegradable aliphatic polyesters and polycarbonates catalyzed by novel versatile aluminum metal complexes bearing salen ligands
Authors: Yang Jing , Yu Yihua , Li Qiaobo , Li Yang , Cao Amin 
Published: December 2004

AbstractTwo novel aluminum metal complexes (2 and 3) bearing salen ligands were in situ prepared from trimethyl aluminum (AlMe3), methanol, and (R,R)‐N,N′‐bis(salicylidene)‐1,2‐diaminocyclohexane with original synthetic strategies, and a preliminarily resoluted (R,R)‐1,2‐diaminocyclohexane was applied as a synthetic precursor. By means of Fourier transform infrared spectrometry, NMR spectrometry, mass spectrometry, and single‐crystal X‐ray diffractometry, 2 and 3 were revealed to be distinct molecular structures with corresponding yields of 85 and 10%, respectively. Further studies via 27Al NMR techniques and single‐crystal X‐ray diffraction indicated that dimeric metal complex 3 appeared in the six‐coordinated state, whereas there was a dynamic equilibrium transition between the five‐ and six‐coordinated states for metal complex 2 in a CDCl3 solution. The more stable dimeric metal complex (3) exhibited two inequivalent aluminum metal centers coordinated to nitrogen atoms attributed to two different salen ligands, and this was different from the reported salen aluminum complex structures. Furthermore, 2 and 3 were employed as candidate catalysts for the ring‐opening polymerization (ROP) of some important biodegradable aliphatic polyesters and polycarbonates, including poly(ϵ‐caprolactone) (PCL), poly(δ‐valerolactone), poly(trimethylene carbonate), and poly(2,2‐dimethyl trimethylene carbonate). The synthetic results indicated that both metal complexes efficiently catalyzed ROP at 100 °C in an anisole solution, and 3 showed much better controlled characteristics of ROP than 2. Very narrow molecular weight distributions close to 1.21 for PCL were detected with 3 as the ROP catalyst. In addition, a catalytic mechanism study confirmed that ROP catalyzed by these metal complexes was in good agreement with the commonly accepted coordination polymerization reported for aluminum triiso [Al(OiPr)3] and stannous octanoate [Sn(Oct)2]. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 373–384, 2005
Sample size recalculation for binary data in internal pilot study designs
Authors: Friede Tim , Kieser Meinhard 
Published: December 2004

AbstractFor binary endpoints, the required sample size depends not only on the known values of significance level, power and clinically relevant difference but also on the overall event rate. However, the overall event rate may vary considerably between studies and, as a consequence, the assumptions made in the planning phase on this nuisance parameter are to a great extent uncertain. The internal pilot study design is an appealing strategy to deal with this problem. Here, the overall event probability is estimated during the ongoing trial based on the pooled data of both treatment groups and, if necessary, the sample size is adjusted accordingly. From a regulatory viewpoint, besides preserving blindness it is required that eventual consequences for the Type I error rate should be explained. We present analytical computations of the actual Type I error rate for the internal pilot study design with binary endpoints and compare them with the actual level of the chi‐square test for the fixed sample size design. A method is given that permits control of the specified significance level for the chi‐square test under blinded sample size recalculation. Furthermore, the properties of the procedure with respect to power and expected sample size are assessed. Throughout the paper, both the situation of equal sample size per group and unequal allocation ratio are considered. The method is illustrated with application to a clinical trial in depression. Copyright © 2004 John Wiley & Sons Ltd.
The carbohydrate‐deficient glycoprotein syndromes: An overview
Authors: Jaeken J. , Carchon H. 
Published: December 2004

SummaryThe carbohydrate‐deficient glycoprotein (CDG) syndromes are a newly recognized family of diseases with autosomal recessive inheritance. The basic defects are probably in the glycosylation pathway (endoplasmic reticulum, Golgi apparatus or post‐Golgi). In the present state of our knowledge the central nervous system is always severely affected but nearly all other organs are involved to a variable degree. Like the peroxisomal disorders they also comprise dysmorphic features, the most typical being an abnormal distribution of subcutaneous adipose tissue. A reliable diagnostic test is isoelectric focusing of serum transferrin showing a cathodal shift as a consequence of the partial sialic acid deficiency. Prenatal diagnosis and heterozygote detection are not yet available. These diseases should be differentiated from secondary CDG syndromes such as classical galactosaemia.
Unreliability of platelet glucose‐6‐phosphatase for the diagnosis of glycogen storage disease type Ia
Authors: Goldberg J. D. , Terleaven S. C. , Koresawa M. , Simpson T. , Golbus M. S. 
Published: December 2004

SummaryThe diagnosis of glycogen storage disease type Ia currently uses enzyme analysis of liver tissue. This requires liver biopsy in the at‐risk neonate or fetus. Conflicting reports have appeared in the literature on the use of peripheral platelet glucose‐6‐phosphatase activity for the diagnosis of this disorder. We have applied a sensitive radiometric assay system to the measurement of glucose‐6‐phosphatase activity in peripheral platelets. Two families with affected members were analysed, revealing no differences in glucose‐6‐phosphatase activity as compared with control values. Platelet measurement of glucose‐6‐phosphatase does not appear to be useful for the diagnosis of glycogen storage disease type Ia.
Radiocarbon Dates of Elevated Shorelines, Indonesia and Malaysia. Part 1
Authors: Tjia H.D. , Fujii Shoji , Kigoshi Kunihiko , Sugimura Arata , Zakaria Thomas 
Published: December 2004

Four new radiocarbon dates of elevated strandlines in tectonically active areas of eastern Indonesia and East Malaysia indicate average rates of uplift that range between 4.5 and 9 mm annually during the past 24,000 yr. These values are at least three times higher than former estimates from eastern Indonesia. Another radiocarbon date from the south arm of Sulawesi—also tectonically mobile—indicates a rate of uplift of 1.4–2.5 mm per year which corresponds with earlier determinations. This particular case, however, suggests that the sample was probably located close to a north-south axis about which southern Sulawesi was tilted during the Quaternary.In the Langkawi islands, West Malaysia, one of the regionally ubiquitous shorelines at about 2 m above sea level was dated at 2590 ± 100 yr BP which corresponds with Fairbridge's (1961) Abrolhos Submergence.
Stratigraphic and Radiocarbon Evidence for the Age and Development of Three Salt Lake Deposits in Rajasthan, India
Authors: Singh Gurdip , Joshi R.D. , Singh A.B. 
Published: December 2004

A series of radiocarbon determinations have been carried out of lacustrine deposits contained in three playa basins, namely at Sambhar, Didwana and Lunkaransar, in Rajasthan, in conjunction with stratigraphical and palynological investigations. It is revealed that the lake deposits, which overlie thick beds of sand at each site, date back from early Holocene times (ca. 10,000 B.P.).The authors present the radiocarbon evidence together with an outline of the stratigraphy of the deposits, and attempt to reconstruct the sedimentary sequence in the three basins since the beginning of the lacustrine phase. The significance of the more or less synchronous development in all the three playa basins is discussed with reference to postglacial climatic oscillations in northwest India.
Lithofacies and Environments of Bed I, Olduvai Gorge, Tanzania
Authors: Hay Richard L. 
Published: December 2004

AbstractBed I in Olduvai Gorge spans the interval from about 1.7 to 2.0 m.y.a., and all evidences of hominid activity at Olduvai are within the latter half of this period. Bed I was deposited in a closed basin approximately 25 km in average diameter, and it can be subdivided into five lithologically different but partly time-equivalent rock assemblages, or lithologic facies, each of which was deposited in the same geographic environment or closely related series of environments. These lithofacies comprise lake deposits, lake-margin deposits, alluvial-fan deposits, alluvial-plain deposits, and lava flows.Alluvial-plain deposits form the lowermost part of Bed I, and they presumably interfinger northeastward with lake deposits in an area not now exposed. Discharge of the lavas diplaced the lake westward to a position it occupied through the latter half of the deposition of Bed I. The lake fluctuated greatly in salinity, level, and extent, and at times of low level it was highly saline and generally ranged between 7 and 10 km in average diameter. At times of high level it was as much as 15 km in diameter and was relatively fresh, at least along its southeastern margin. The lake-margin deposits accumulated on the zone of relatively flat terrain that was flooded at times of high level. Lake-margin deposits interfinger eastward with deposits of an alluvial fan of pyroclastic materials produced in an explosive phase of the volcano Olmoti. The climate at the time of Bed I was relatively dry although somewhat wetter than the present climate in the same region today.
Holocene Climatic Variations Inferred from Treeline Fluctuations in the White Mountains, California
Authors: LaMarche Valmore C. 
Published: December 2004

AbstractRemains of dead bristlecone pine (Pinus longaeva Bailey) are found at altitudes up to 150 m above present treeline in the White Mountains. Standing snags and remnants in two study areas were mapped and sampled for dating by tree-ring and radiocarbon methods. The oldest remnants represent trees established more than 7400 y.a. Experimental and empirical evidence indicates that the position of the treeline is closely related to warm-season temperatures, but that precipitation may also be important in at least one of the areas. The upper treeline was at high levels in both areas until after about 2200 B.C., indicating warm-season temperatures about 3.5°F higher than those of the past few hundred years. However, the record is incomplete, relative warmth may have been maintained until at least 1500 B.C. Cooler and wetter conditions are indicated for the period 1500 B.C.-500 B.C., followed by a period of cool but drier climate. A major treeline decline occurred between about A.D. 1100 and A.D. 1500, probably reflecting onset of cold and dry conditions. High reproduction rates and establishment of scattered seedlings at high altitudes within the past 100 yr represents an incipient treeline advance, which reflected a general climatic warming beginning in the mid-19th century that has lasted until recent decades in the western United States. This evidence for climatic variation is broadly consistent with the record of Neoglacial advances in the North American Cordillera, and supports Antevs' concept of a warm “altithermal age” in the Great Basin.
Astronomical Theory of Climatic Change: Status and Problem
Authors: Chappell J. 
Published: December 2004

AbstractQuaternary paleotemperatures and sea level records, from both deep sea cores and dated shorelines, provide the basis for testing the Milankovitch hypothesis of climatic change. The longest and most detailed records include (1) oxygen isotope analyses of Caribbean and Atlantic deep sea cores, (2) paleoecological analyses of the same cores, and (3) radiometrically dated raised coral reefs from New Guinea and elsewhere, representing times of relatively high Quaternary sea levels. Time-domain and frequency-domain analysis of these records, shows with a high degree of certainty that Quaternary climatic changes are strongly influenced by the obliquity perturbations and precession of the Earth's orbit. The same analyses also suggest that the time scale adopted by Emiliani for deep sea cores may be more nearly correct than alternative time scales of other workers.The question of whether insolation changes arising from orbital perturbations can generate ice ages, has been disputed by climatologists. It is shown here that orbital perturbations cannot affect climate indirectly through agencies originating within the Earth, such as vulcanism, and that the primary climatic control is therefore through variation of insolation distribution, as Milankovitch suggested. The conclusion is that climatologic theory must accommodate these facts.
The Vegetation Record of a Mid-Wisconsin Interstadial in Northwest Georgia
Authors: Watts W.A. 
Published: December 2004

AbstractAt Green Pond, a small permanent sinkhole pond in Bartow County, northwest Georgia, organic silty clays are buried by up to 2 m of colluvium. Pollen from the clays shows that a Pinus-Quercus-herb (pine-oak-herb) flora was present before 29,630 radiocarbon yr ago. It is interpreted as the product of a xeric woodland with prairie-like openings. Between 29,630 and approximately 25,000 BP, pollen of Pinus and herbs was sparse; Quercus and Carya (hickory) predominated in the pollen rain. There were few other deciduous trees. Oak-hickory forest is thought to have been present. From 25,000 to 23,000 BP, more diverse forest with pines and some Picea (spruce) became established. At the same time Taxodium (swamp cypress) was locally abundant, as were shrubs characteristic of Coastal Plain swamps. Some time after 23,000 BP, the pond basin filled with colluvium and no further sedimentation took place, other than thin muck sedimented on the bottom of the present Green Pond.The sediments were first thought to be of Sangamon age because the pollen sequence has many of the characteristics of an interglacial cycle, but the radiocarbon dates correlate them firmly with the Farmdalian Interstadial. A comparison with known Farmdalian sites is made, but the important sites are in the northern United States and adjacent Canada, too far away to make a useful comparison of the details of pollen diagrams from the two areas. At another Bartow County pond site, Bob Black Pond (Watts, 1970), a flora predominantly of pine with spruce and oak was present immediately before 22,900 BP and a strikingly cold flora with jack-pine, spruce and northern herbs followed immediately after. The radiocarbon dates indicate that the sedimentary sequence at Bob Black Pond immediately follows that at Green Pond.
Environmental Sequence following the Fraser Advance of the Juan de Fuca Lobe, Washington
Authors: Heusser Calvin J. 
Published: December 2004

AbstractStratigraphic palynology and radiocarbon chronology of two bogs and a lake on the northwestern Olympic Peninsula serve to record the environmental sequence postdating the Fraser maximum of the Juan de Fuca lobe. Wastage of the lobe in the terminal area began before 14,460 ± 200 BP. Differential downwasting followed, and the last remnants of dead ice probably disappeared some time before 9,380 ± 180 BP. Ablation moraine became sufficiently thick in the course of wastage for a vegetation cover to become established. Arboreal remains of this cover, found buried in till, date between 12,020 ± 210 and 13,380 ± 250 BP.Communities of Pinus contorta first succeeded on deglaciated surfaces during the Vashon Stade. Environmental conditions were comparable to those prevailing in the modern subalpine forest, and average July temperature stood near 12°C. Later, during the Everson Interstade (11,000–13,000 BP.), Alnus and Picea multiplied as temperature increased posibly to as much as 14°. During the Sumas Stade (10,000–11,000 BP.), temperature was again ca. 12°, the cooler climate halting wastage and the spread of Alnus and enabling communities of Picea, Tsuga heterophylla, and T. mertensiana to temporarily achieve stability.Postglacial environments through the Hypsithermal (ca. 3,000 BP) were dominated principally by Alnus. Alnus, succeeded in turn by Picea, invaded the landscape, following the recession of alpine glaciers and the rise in elevation of the snowline. For a time, as suggested by a peak of Pseudotsuga, temperature may have reached close to 17° and annual precipitation less than 760 mm. Arboreal communities were relatively open while light-requiring Pteridium remained conspicuous in the record. After 3000 BP during Neoglaciation, climate became sufficiently cool and moist to favor the development of extensive, closed communities of Tsuga, Picea, Thuja, and other hygrophilous species.
The Antiquity of Man in South America
Authors: Lynch Thomas F. 
Published: December 2004

The initial peopling of South America is reviewed in terms of geochronological and archaeological data. The problem is put in historical perspective and a systematic evaluation is made of the latest typologically oriented arguments for the presence of preprojectile point complexes. Field evidence is very weak for early Biface and Chopper traditions. There is better support for an early Flake tradition, but it is not likely to precede the use of stone projectile points.Radiocarbon dates and “terrace dating” should be used only with great caution and careful attention to the nature of cultural and stratigraphic associations. Uncritical acceptance of determinations on bone and soil samples has led to extremely problematic cultural reconstructions. Emphasis on stratified sites with finished artifacts, faunal assocations, and charcoal dates leads to a reconstruction at odds with those presented in recent textbooks. It is concluded that the bulk of solid evidence favors the entry of man as a big game hunter using stone projectile points and having an adaptation much like that of the Paleo-Indians of North America.