bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calcium
Authors: Silliman Christopher C , Elzi David J , Ambruso Daniel R , Musters Rene J , Hamiel Christine , Harbeck Ronald J , Paterson Andrew J , Bjornsen A Jason , Wyman Travis H , Kelher Marguerite , England Kelly M , McLaughlin-Malaxecheberria Nathan , Barnett Carlton C , Aiboshi Junichi , Bannerjee Anirban 
Published: March 2003

Abstract
A mixture of lysophosphatidylcholines (lyso-PCs) are generated during blood storage and are etiologic in models of acute lung injury. We hypothesize that lyso-PCs stimulate polymorphonuclear neutrophils (PMNs) through Ca2+-dependent signaling. The lyso-PC mix (0.45–14.5 μM) and the individual lyso-PCs primed formyl-Met-Leu-Phe (fMLP) activation of the oxidase (1.8- to 15.7-fold and 1.7- to 14.8-fold; P&lt;0.05). Labeled lyso-PCs demonstrated a membrane association with PMNs and caused rapid increases in cytosolic Ca2+. Receptor desensitization studies implicated a common receptor or a family of receptors for the observed lyso-PC-mediated changes in PMN priming, and cytosolic Ca2+ functions were pertussis toxin-sensitive. Lyso-PCs caused rapid serine phosphorylation of a 68-kD protein but did not activate mitogen-activated protein kinases or cause changes in tyrosine phosphorylation. With respect to alterations in PMN function, lyso-PCs caused PMN adherence, increased expression of CD11b and the fMLP receptor, reduced chemotaxis, provoked changes in morphology, elicited degranulation, and augmented fMLP-induced azurophilic degranulation (P&lt;0.05). Cytosolic Ca2+ chelation inhibited lyso-PC-mediated priming of the oxidase, CD11b surface expression, changes in PMN morphology, and serine phosphorylation of the 68-kD protein. In conclusion, lyso-PCs affect multiple PMN functions in a Ca2+-dependent manner that involves the activation of a pertussis toxin-sensitive G-protein.
Endogenous versus exogenous glucocorticoid responses to experimental bacterial sepsis
Authors: Silverstein Richard , Johnson Donald C 
Published: March 2003

Abstract
Although lack of adrenals dramatically reduces resistance against sepsis generally, the value of glucocorticoid levels above those normally produced by stress remains controversial. An early and long-held concept is that glucocorticoid protection against lipopolysaccharides in animal models is important. Supporting this concept, C3H/HeJ mice, lacking Toll-like receptor-4 (TLR-4), and consequently, endotoxin hyporesponsive, have recently been shown to be resistant to glucocorticoid protection against live Escherichia coli. Effective antibiotic intervention, as an additional parameter and with concomitant administration of glucocorticoid, not only allows for expected antibiotic protection but also for glucocorticoid protection against E. coli or Staphylococcus aureus of mice sensitized to tumor necrosis factor α, regardless of the status of the TLR-4 receptor. TLRs, including but not limited to TLR-2, may be involved in glucocorticoid protective efficacy against Gram-positive and Gram-negative sepsis. Overlapping and possibly endotoxin-independent signaling may become important considerations.
Functional modulation of dendritic cells to suppress adaptive immune responses
Authors: Woltman Andrea M , van Kooten Cees 
Published: March 2003

AbstractIn recent years, dendritic cells (DCs) have entered the center court of immune regulation. Dependent on their ontogeny, state of differentiation, and maturation and thereby a variable expression of membrane-bound and soluble molecules, DCs can induce immunostimulatory as well as immunoregulatory responses. This dual function has made them potential targets in vaccine development in cancer and infections as well as for the prevention and treatment of allograft rejection and autoimmune diseases. The present review is focused on the effect of immune-modulatory factors, such as cytokines and immunosuppressive drugs, and on the survival, differentiation, migration, and maturation of DC human subsets. A better understanding of DC immunobiology may lead to the development of specific therapies to prevent or dampen immune responses.
Involvement of P2Y receptors in the differentiation of haematopoietic cells
Authors: Sak Katrin , Boeynaems Jean-Marie , Everaus Hele 
Published: March 2003

Abstract
The effects of extracellular nucleotides are mediated by multiple P2X ionotropic receptors and G protein-coupled P2Y receptors. These receptors are ubiquitous, but few physiological roles have been firmly identified. In this review article, we present a survey of the functional expression of P2Y receptors in the different haematopoietic lineages by analyzing the selectivity of these cells for the various adenine and uracil nucleotides as well as the second messenger signaling pathways involved. The pharmacological profiles of metabotropic nucleotide receptors are different among myeloid, megakaryoid, erythroid, and lymphoid cells and change during differentiation. A role of P2Y receptors in the differentiation and maturation of blood cells has been proposed: In particular the P2Y11receptor seems to be involved in the granulocytic differentiation of promyelocytes and in the maturation of monocyte-derived dendritic cells. It is suggested that the role of P2Y receptors in the maturation of blood cells may be more important than believed so far.
Development and Validation of a Patient-Reported Outcome Measure to Assess Disease Control in Chronic Prurigo
Authors: Metz Martin , Zeidler Claudia , Hawro Tomasz , Pereira Manuel , Maurer Marcus , Bonnekoh Hanna , Krause Karoline , Pritchard Thomas , Kwatra Shawn G. , Ständer Sonja , Weller Karsten 
Published: January 2024

ImportanceChronic prurigo (CPG), including prurigo nodularis, is a difficult disease to treat and considerably affects patients’ quality of life. Helping patients obtain control of CPG is a major treatment goal.ObjectiveTo develop and validate the Prurigo Control Test (PCT), a tool for assessing disease control in CPG, and to identify a cutoff value for controlled disease to aid treatment decisions.Design, Setting, and ParticipantsThis qualitative study followed the current recommendations for patient-reported outcome measure development in the generation and validation of the PCT. The final PCT was obtained after item generation, followed by reduction and selection, and was then tested for internal consistency and test-retest reliability, convergent validity, known-group validity, screening accuracy, and banding. The item-generation phase resulted in an unselected list of 69 potential PCT items. Impact analysis, interitem correlation, and review for content (face) validity resulted in final set of 5 PCT items. The validation study was performed among patients across 2 expert centers in Germany. Data were analyzed from February 2017 to November 2019.Main Outcomes and MeasuresA 5-item PCT with a recall period of 2 weeks was developed. A cutoff value of 10 points or higher was determined as suitable for identifying patients with well-controlled vs poorly controlled CPG.ResultsOf the 95 patients included in the validation study, the median (range) age was 63 (19-87) years, 50 patients (53%) were women, and the median (range) disease duration was 72 (9-774) months. The validation study yielded good internal consistency reliability (Cronbach α, 0.86) and a high degree of convergent validity. The PCT demonstrated good known-group validity and could discriminate between patients who differed in prurigo control. Test-retest reliability was high, and the intraclass correlation coefficient was 0.94, indicating excellent reproducibility.Conclusions and RelevanceThis qualitative study showed that the PCT is able to assess disease control in patients with CPG. Its retrospective approach, brevity, and simple scoring likely make the PCT suitable for clinical practice and trials.
Safety and Feasibility of Single-Port Robotic-Assisted Nipple-Sparing Mastectomy
Authors: Farr Deborah E. , Haddock Nicholas T. , Tellez Juan , Radi Imad , Alterio Rodrigo , Sayers Brendan , Zeh Herbert 
Published: January 2024

ImportanceRobotic-assisted nipple-sparing mastectomies with multiport robots have been described in the US since 2015; however, significant hurdles to multiport robotic surgery exist in breast surgery.ObjectiveTo demonstrate that the single-port da Vinci SP (Intuitive Surgical) robotic system is feasible in patients undergoing robotic nipple-sparing mastectomy (rNSM).Design, Setting, and ParticipantsAn initial case series of 20 patients at a large university hospital underwent bilateral single-port robotic nipple-sparing mastectomies (SPrNSM) with tissue expander reconstruction from February 1, 2020, through January 4, 2023. Participants included women who met surgical criteria for nipple-sparing mastectomies, per standard of care.InterventionSurgery using a single-port robot and the surgical technique of the authors.Main Outcomes and MeasuresAge, indication, body mass index, breast size, operative time, conversion to open surgery, systemic complications, postoperative skin necrosis, and reported skin and nipple areolar complex (NAC) sensation.ResultsTwenty women aged 29 to 63 years (median, 40 years) underwent bilateral SPrNSM. Eleven patients completed prophylactic surgery due to a high risk for breast cancer (more than 20% lifetime risk) and 9 patients had breast cancer. Breast size ranged from A through D cup with median B cup and a body mass index range of 19.7 through 27.8 (median 24.4). The total duration of the procedure from incision to skin closure for both sides ranged from 205 minutes to 351 minutes (median, 277). The median robotic time for bilateral SPrNSM was 116 minutes and varied by cup size (A cup, 95 minutes; B cup, 140 minutes; C cup, 118 minutes; D cup, 114 minutes) with no inflection point in learning curve. No cases were converted to open and no immediate complications, such as hematoma, positive margins, or recurrence, were seen. In the first 10 patients prior to routine sensation testing, 20 resected breasts had measurable NAC sensation at a range from 4 to 36 months post–index resection (65%). In the second 10 patients of the cohort, measurable NAC was preserved in 13 of 20 resected breasts 2 weeks following the index operation (65%).Conclusion and RelevanceIn this case series, SPrNSM with immediate reconstruction was feasible and performed safely by an experienced breast surgeon with limited previous robotic training. Further studies confirming the preliminary data demonstrating improved NAC and skin sensation following SPrNSM are warranted.Trial RegistrationClinicalTrials.gov Identifier: NCT05245812
Is your memory better than mine? Investigating the mechanisms and determinants of the memory conformity effect using a modified MORI technique
Authors: Kękuś Magdalena , Polczyk Romuald , Ito Hiroshi , Mori Kazuo , Barzykowski Krystian 
Published: January 2024

AbstractThe paper presents the memory conformity effect phenomenon, which involves the inclusion in memory accounts concerning a particular event (original information) of incorrect information (misinformation) that a witness has obtained as a result of another witness's account of the same event. The research had two goals: (1) to verify the existence of individuals who yield to misinformation yet are aware of discrepancy between the original information and the misinformation; (2) to determine why these individuals choose misinformation despite the correctness of their own memories. In addition, we examined the relationship between susceptibility to social influence, compliance, suggestibility and memory conformity in interaction with awareness of discrepancy. In order to examine the memory conformity effect, we used the MORI technique, which ensures high ecological validity. In this technique, the two members of each pair of participants sit next to each other and each are not aware that the other is watching a different version of the same event. Then, the participants answer related questions and discuss the contradictory details. Subsequently, the subjects complete an individual memory test. Importantly, after the main part of the study (i.e., the MORI procedure), participants were explicitly informed about the different versions of the event, and they were asked to complete awareness of discrepancy questionnaires. It was shown that awareness of the discrepancy between the original information and the misinformation reduced succumbing to misinformation. However, it was demonstrated that, despite being aware of discrepancies, 21.4% participants still succumbed to the misinformation. It was also shown that the main reason for participants being misinformed despite being aware of the discrepancy was distrust of their own memory.
The Relationship between Dietary Macronutrient Composition and Telomere Length Among US Adults
Authors: Koemel Nicholas A. , Laouali Nasser , Senior Alistair M. , Celermajer David S. , Grech Amanda , Solon‐Biet Samantha M. , Simpson Stephen J. , Raubenheimer David , Gill Timothy P. , Skilton Michael R. 
Published: January 2024

AbstractThe role of dietary macronutrients and energy intake in the aging process has been well‐established. However, previous research has mainly focused on the association between leukocyte telomere length (LTL) and individual macronutrients, while the effects of macronutrient composition on LTL remain unclear. This cross‐sectional analysis involved 4130 US adults (44.8 ± 17.0 years; 51% female) from the National Health and Nutrition Examination Survey during 1999–2002. A single 24‐h dietary recall is used to collect dietary data. The relationship between dietary macronutrient composition and LTL is examined using three‐dimensional generalized additive models. After adjustment for age, sex, ethnicity, education, physical activity, BMI, and dietary quality, a three‐dimensional association of macronutrient composition with LTL (P = 0.02) is revealed. Diets lower in protein (5–10%), higher in carbohydrates (75%), and lower in fat (15–20%) are associated with the longest LTL corresponding to 7.7 years of slower biological aging. Diets lowest in protein (5%) and carbohydrate (40%), while highest in dietary fat (55%) are associated with the shortest LTL, corresponding to accelerated biological aging of 4.4 years. The associations appeared magnified with higher energy intake. These findings support a complex relationship between dietary macronutrients and biological aging independent of diet quality.
Amino‐Functionalized Graphdiyne Derivative as a Cathode Interface Layer with High Thickness Tolerance for Highly Efficient Organic Solar Cells
Authors: Kan Yuanyuan , Sun Yanna , Ren Yi , Xu Yixuan , Jiang Xinyue , Shen Haojiang , Geng Longlong , Li Jianfeng , Cai Ping , Xu Huajun , Gao Ke , Li Yuliang 
Published: January 2024

AbstractEfficient cathode interfacial materials (CIMs) are essential components for effectively enhancing the performance of organic solar cells (OSCs). Although high‐performance CIMs are desired to meet the requirements of various OSCs, potential candidates for CIMs are scarce. Herein, an amino‐functionalized graphdiyne derivative (GDY‐N) is developed, which represents the first example of GDY that exhibits favorable solubility in alcohol. Utilizing GDY‐N as the CIM, an outstanding champion PCE of 19.30% for devices based on the D18‐Cl:L8‐BO (certified result: 19.05%) is achieved, which is among the highest efficiencies reported to date in OSCs. Remarkably, the devices based on GDY‐N exhibit a thickness‐insensitive characteristic, maintaining 95% of their initial efficiency even with a film thickness of 25 nm. Moreover, the GDY‐N displays wide universality and facilitates exceptional stability in OSCs. This work not only enriches the diversity of GDY derivatives, but also demonstrates the feasibility of GDY derivatives as CIMs with high thickness tolerance in OSCs.
All‐Carbon‐Based Complementary Integrated Circuits
Authors: Watanabe Kazuyoshi , Miura Naoki , Taguchi Hiroaki , Komatsu Takeshi , Aratake Atsushi , Makita Tatsuyuki , Tanabe Masahiro , Wakimoto Takahiro , Kumagai Shohei , Okamoto Toshihiro , Watanabe Shun , Takeya Jun 
Published: January 2024

AbstractOwing to the growing global increase in electronic and electrical waste (e‐waste), significant challenges arise regarding the proper disposal of electronic devices. One potential solution that has gained attention is the development of disposable electronics, in which all components are designed to be for safe and environmentally friendly disposal. In this study, all‐carbon‐based complementary integrated circuits composed of printed single‐crystalline thin films of p‐ and n‐type organic semiconductors, graphite‐based carbon electrodes/wiring, and polymeric dielectrics/substrates are demonstrated. Elemental analysis reveals that the total amount of metallic element contaminants weighed <50 parts per million (ppm). The demonstrated analog/digital integrated circuits with 64 p‐ and n‐type organic thin‐film transistors exhibit stable operation under ambient environmental conditions. These all‐carbon‐based complementary integrated circuits possess excellent element traceability, thus mitigating the potential environmental impacts throughout the device's life cycle. Consequently, this advancement represents a significant step toward addressing the global environmental challenges associated with e‐waste.
Organocatalytic Remote Stereocontrolled Synthesis of Tetrasubstituted Allenes via Asymmetric 1,8‐Addition of Propargylic Aza‐p‐Quinone Methides
Authors: Yue Zhibin , Xia Yan , Shen Boming , Liu Chang , Yu Peiyuan , Li Pengfei , Li Wenjun 
Published: January 2024

AbstractAn organocatalytic remote stereocontrolled 1,8‐conjugated addition of in situ formed propargylic aza‐p‐QMs from α‐(4‐aminophenyl) propargylic alcohols and indole‐2‐carboxylates was developed, affording axially chiral tetrasubstituted allenes in 62–99% yield with 52–99% ee. The synthetic strategy not only enriches the chemistry of aza‐p‐quinone methides, but also provides an alternative tool for the preparation of axially chiral tetrasubstituted allenes.
Bomb pulse 14C evidence for consistent remodeling rates of cortical femur collagen in middle‐late adulthood
Authors: Quinn Rhonda L. 
Published: January 2024

AbstractObjectivesBomb pulse (BP) radiocarbon (14C) dating methods are used by forensic anthropologists to estimate the year‐of‐death (YOD) of unidentified individuals. Method resolution and accuracy depend on establishing lag times, or the difference between a tissue's BP 14C‐derived year and the YOD, of various tissue types from known deceased persons. Bone lag times span many years and are thought to increase with age as a function of slowing remodeling rates. However, remodeling rates for various skeletal elements, bone structures and phases are not well known.Materials and MethodsHere a simple method is used to estimate bone remodeling rates from a compilation of published cortical femur bone collagen BP 14C measurements (n = 102). Linear regression models and nonparametric tests are used to detect changes in lag times and remodeling rates with increasing age‐at‐death.ResultsRemodeling rates and lag times of 3.5%/year and 29 years, respectively, are estimated from individuals aged 40–97 years. In contrast to previous work, the analysis yielded modest and negligible changes in remodeling rates and lag times with advancing age. Moreover, statistically significant differences in remodeling rates and lag times were not found between reported females and males.DiscussionImplications for the temporal contexts within an individual's lifetime of biogeochemical data in archaeology and forensic anthropology are discussed, warranting additional BP 14C studies of known individuals and integration with histomorphometric analysis.
Sulfonated Perylene as Three‐in‐One STING Agonist for Cancer Chemo‐Immunotherapy
Authors: Zhao Xuejie , Zheng Rijie , Zhang Bianbian , Zhao Ying , Xue Wanli , Fang Yingfei , Huang Yongwei , Yin Meizhen 
Published: January 2024

AbstractActivation of stimulator of interferon genes (STING) by cyclic dinucleotides (CDNs) has been considered as a powerful immunotherapy strategy. While promising, the clinical translation of CDNs is still overwhelmed by its limited biostability and the resulting systemic immunotoxicity. Being differentiating from current application of exogenous CDNs to address these challenges, we herein developed one perylene STING agonist PDIC‐NS, which not only promotes the production of endogenous CDNs but also inhibits its hydrolysis. More significantly, PDIC‐NS can well reach lung‐selective enrichment, and thus mitigates the systemic immunotoxicity upon intravenous administration. As a result, PDIC‐NS had realized remarkable in vivo antitumor activity, and backward verified on STING knock out mice. Overall, this study states that PDIC‐NS can function as three‐in‐one small‐molecule STING agonist characterized by promoting the content and biostability of endogenous CDNs as well as possessing good tissue specificity, and hence presents an innovative strategy and platform for tumor chemo‐immunotherapy.
Probing the interface structure of block copolymer compatibilizers in semicrystalline polymer blends
Authors: Tang Xiaomin , Liu Changhao , Chen Jihua , Kumar Rajeev , Bowland Christopher C. , Saito Tomonori , Dial Brent E. , Keum Jong K. , Do Changwoo , Chen Xi Chelsea 
Published: January 2024

AbstractThe use of block copolymers to compatibilize immiscible plastics is an important strategy for upcycling municipal plastic wastes. Multiblock copolymers (MBCPs) have been proven to be more effective compatibilizers than di‐ and tri‐block copolymers. Herein, we probe the interface structure of an effective multiblock copolymer compatibilizer and compare that with an ineffective triblock copolymer (TBCP). The interface activity of the compatibilizers is understood through a combination of small‐angle neutron and x‐ray scatterings (SANS and SAXS), by using deuterated homopolymer matrix and protonated compatibilizers. SANS analysis suggests that the MBCP forms a thicker interface layer (7–9 nm) than the TBCP (0–4 nm). In addition, SANS data seems to point to a stronger tendency for the MBCP to locate at the interface. Both factors contribute to its effectiveness at compatibilizing immiscible homopolymers.
Enhancement of fire safety in epoxy resin composites through incorporation of microencapsulated diatomite
Authors: Wang Jin , Shen Xiaoyue , Jiang Mengwei , Zhou Fang , Zhang Yupeng 
Published: January 2024

AbstractDiatomite (DIA) particles are commonly employed as flame‐retardant additives for polymers, yet their intrinsic inefficiency requires substantial quantities for optimal efficacy. To address this issue, we proposed a novel approach involving the microencapsulation of DIA with polyethylene glycol phosphate (PEGP) to enhance the flame retardancy of epoxy resin (EP). Characterization of the prepared DIA@PEGP utilized scanning electron microscopy with energy‐dispersive x‐ray spectrometry and Fourier transform infrared spectroscopy. The resulting EP composite, DIA@PEGP‐4/EP, achieved a limiting oxygen index of 33.2% and achieved a V‐0 level in vertical combustion tests. Compared to EP, DIA@PEGP‐4/EP demonstrated significantly improved fire performance, with 38.6%, 47.8%, 25.0%, 41.3%, and 60.4% reduction in peak heat release rate, total heat release, peak smoke production rate, total smoke production, and CO yield. Furthermore, the highest FPI value of 0.080 m2·s/kW for DIA@PEGP‐1/EP and the lowest FGI value of 8.734 kW/m2·s for DIA@PEGP‐4/EP, indicate that the incorporation of DIA@PEGP into EP enhances its fire safety. The flame retardancy mechanism of DIA@PEGP‐4 involves the formation of a phosphorus‐containing aromatic carbon layer during EP char formation, capturing radicals in the gas phase during combustion.