bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yeast (different sources and levels) as protein source in diets of reared piglets: effects on protein digestibility and N‐metabolism
Authors: Spark M. , Paschertz H. , Kamphues J. 
Published: March 2005

SummaryThe aim of this study was to examine the feeding value of different yeasts as a substitute for soya bean meal, the main protein source in diets of weaned piglets. Tested two yeasts were already available on the market, Saccharomyces cerevisiae and Kluyveromyces lactis (beer and milk yeast), which replaced 40% of the soya bean meal in the diets. Furthermore, a yeast (Kluyveromyces fragilis) grown on whey, a side‐product of cheese production, was used in increasing concentrations in the diets, so that increasing amounts of the soya bean meal (20%, 40% and 60%) could be replaced. As proved in these experiments, a replacement of 60% of the soya protein with whey yeast protein had positive effects on the performances (daily weight gain) and on the N‐metabolism and did not have negative effects on the health or the faeces consistency. The whey yeast stands out because of its high protein quality (N‐digestibility and N‐retention). Furthermore, the replacement of soya bean meal with highly digestible yeasts is welcomed under the aspect of animal health, because of the reduction of anti‐nutritive soya components (stachyose, glycinin) in diets of weaned piglets. The controlled production conditions of the yeasts result in a high feed safety; in addition, the yeast as an end‐of‐pipe‐product is a resource conserving and valuable feed. A main stimulus for the use of yeasts, however, in a food production controlled by economic standpoints, is their price and the costs of other competing feeds.
Amino acid concentrations in blood serum of horses performing long lasting low‐intensity exercise
Authors: Bergero D. , Assenza A. , Schiavone A. , Piccione G. , Perona G. , Caola G. 
Published: March 2005

SummaryThe aim of this work was to evaluate the changes in the concentrations, after two rides different for distance covered, of different amino acids in endurance horses. Blood samples have been collected from horses just before the start, at the top of a steep slope (819 m difference in height) and just at the end of a 32‐km endurance ride. A second group, competing in a 72 km endurance ride, has also been sampled immediately before and after the race. In serum samples, the concentrations of alanine, arginine, asparagine, glycine, isoleucine, histidine, leucine, lysine, methionine, ornithine, phenylalanine, tryptophan, tyrosine and valine have been measured by high‐performance liquid chromatography (HPLC). anova and t‐test have been used to study the differences in the concentrations of the amino acids. The pre‐ride concentrations of the free amino acids were different between the two races, except for methionine and leucine. Differences between start and end race have been found for both groups for all the considered parameters except asparagine, isoleucine, leucine and lysine for the 72 km ride. Increases have been recorded for the shorter and decreases for the longer ride in the blood serum concentrations. Significant increases have also been found between the starting sampling and the second, at the top of the slope, only for alanine, arginine, asparagines, phenylalanine and lysine. The ride length has a significant impact on blood serum amino acids mobilization and uptake; in the shorter race the increases stand only for mobilization, whereas in the longer the decrease can be considered the effect of the onset of the amino acids catabolism.
Two Genetically Distinct Populations of Colletotrichum gloeosporioides Penz. Causing Anthracnose Disease of Yam (Dioscorea spp.)
Authors: Abang M. M. , Fagbola O. , Smalla K. , Winter S. 
Published: March 2005

AbstractVariation within Colletotrichum gloeosporioides, the causal agent of yam anthracnose disease, is still poorly defined and this hinders breeding for resistance. Two morphotypes of C. gloeosporioides, designated slow‐growing grey (SGG) and fast‐growing salmon (FGS), are associated with anthracnose disease of yam in Nigeria. The morphotypes are distinguishable based on colony and conidial morphology, growth rate, virulence, as well as vegetative compatibility, but molecular differentiation of SGG and FGS strains is needed to facilitate epidemiological studies. Denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)‐amplified small subunit (18S) rDNA fragments, and microsatellite‐primed PCR (MP‐PCR) genomic fingerprinting were employed to provide a basis for molecular differentiation of the morphotypes. DGGE analysis revealed patterns that clearly differentiated isolates of the aggressive defoliating SGG from the moderately virulent non‐defoliating FGS strains. Genetic analysis based on 52 MP‐PCR markers revealed highly significant differentiation between the SGG and FGS populations on yam (GST = 0.22; Nei's genetic identity = 0.85; θ = 0.28, P < 0.001), indicating that the SGG and FGS morphotypes represent genetically differentiated populations. The results of the molecular typing using DGGE and MP‐PCR analyses were consistent with the disease phenotype caused by the two morphotypes. Consequently, these molecular techniques might be used, at least partly, to replace time‐consuming virulence studies on yam.
Control of Root Rot and Wilt caused by Pythium myriotylum in Kangaroo Paw (Anigozanthos)
Authors: Tsror (Lahkim) L. , Hazanovsky M. , Mordechai‐Lebiush S. , Ben‐David T. , Dori I. , Matan E. 
Published: March 2005

AbstractControl of root rot and wilt on Kangaroo Paw (Anigozanthos) caused by Pythium myriotylum was tested, using artificial inoculations, in greenhouse experiments. Disease incidence in the inoculated but non‐treated plots was 74% in Expt A (1999) and 46% in Expt B (2000). Disease incidence was significantly reduced by treatments with fenamidon (26 and 70%) and Terraclor SuperX (65 and 41%) in Expts A and B, respectively, in comparison with the non‐treated plots. Disease incidence was reduced with Ridomil Gold by 7 and 48% in Expts A and B, respectively; with Dynone by 22 and 41% in Expts A and B, respectively; with Tachigaren by 20% in both experiments; and with Trichoderma harzianum treatment, disease incidence was reduced by 9% only in Expt B. Yields (recorded only in Expt B) obtained in plots inoculated with P. myriotylum were significantly lower than in the non‐inoculated control plots. The most efficient treatment was fenamidon, with 324% more flowers than in the non‐treated control. The number of flowers in terraclor SuperX, Ridomil Gold, Dynone and T. harzianum treatments was higher by 285, 249, 234 and 235% than in the non‐treated control. In vitro tests demonstrated that the most effective doses, causing 50% inhibition of mycelial growth (ED50), were 0.09, 0.4, 0.4 and 4 μg/ml for the fungicides fenamidon, Ridomil Gold (mefenoxam), Terraclor SuperX (etridiazole + quintozene) and Dynone (prothiocarb), respectively.
Water in the Environment/Socio-Economic Development Discourse: Sustainability, Changing Management Paradigms and Policy Responses in a Global System
Authors: Allan J. A. 
Published: March 2005

AbstractThis paper provides narrative and analytical frameworks for understanding the changing approaches to the valuation, allocation and the management of water resources in the North and the South. The study emphasizes the allocative politics of this important element of environmental capital and its combination with other capitals over the last 50 years. In examining these allocative politics, the role of the concept of sustainability is demonstrated. Discursive hydro-politics are shown to mediate the contested claims on the freshwater resources required by society, the economy and the environment. An historical narrative illustrates five water management paradigms and how they conform to modernity theory. Cultural theory is shown to be particularly relevant for those wanting to support the recently evolved fifth paradigm approach to the allocation and management of water. The theory is supported by reference to the water-short Middle East and North Africa and the dialogue on large dams. The article concludes that a hydro-centric approach is inadequate; water management challenges are associated with complex and sometimes wonderfully invisible processes integral to a political economy.
Rice, Oil and the Atom: A Study of the Role of Key Material Resources in the Security and Development of Japan
Authors: Kelly Dominic 
Published: March 2005

AbstractThis article examines the impact on Japan's political economy and foreign policy of its lack of natural resources. Applying the concept of Japan as a ‘reactive’ state to linked case studies of rice, oil and atomic power it explores aspects of the relationship between culture, institutions and political processes in domestic politics and foreign policy. In so doing it argues that Japan's poor resource endowments have driven it to engage (re)actively – and often unwisely – in international affairs, an engagement both facilitated and constrained by its close alliance with the United States. This mediated engagement will continue into the foreseeable future.
A comparison of methods for determining genotypes at the tumour necrosis factor‐α−308, interleukin (IL)‐1β+3953, IL‐6 −174 and IL‐10 −1082/−819/−592 polymorphic loci
Authors: Bown M. J. , Weston S. , Horsburgh T. , Nicholson M. L. , Bell P. R. F. , Sayers R. D. 
Published: March 2005

SummaryInduced heteroduplex genotyping (IHG) is one of many methods that can be used to determine single nucleotide polymorphisms (SNPs). It is relatively new in comparison to other polymerase chain reaction (PCR)‐based techniques. The aim of this study was to compare the results of genotyping using IHG with the results of genotyping using either polymerase chain reaction–sequence‐specific primers (PCR‐SSP) or polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) for SNPs in the tumour necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐6 and IL‐10 genes. Ninety patients who consented to participate in the study had their genotypes determined by IHG and either PCR‐SSP (TNF‐α−308 and IL‐10 −1082/−819/−592) or PCR‐RFLP (IL‐1β+3953 and IL‐6 −174). Results for each locus were compared between techniques by calculating the Kappa statistic as a measure of agreement. The IHG and more traditional genotyping methods produced very similar results at all loci. The Kappa statistics for each locus were as follows: TNF‐α−308, K = 0.727; IL‐1β+3953, K = 0.886; IL‐6 −174, K = 0.909; IL‐10 −1082, K = 0.876; IL‐10 −592, K = 0.920. IHG is a valid method for the determination of genotypes at the loci examined in this study and produces comparable results to those of more traditional methods of genotyping.
Analysis of Genes Induced by Sendai Virus Infection of Mutant Cell Lines Reveals Essential Roles of Interferon Regulatory Factor 3, NF-κB, and Interferon but Not Toll-Like Receptor 3
Authors: Elco Christopher P. , Guenther Jeanna M. , Williams Bryan R. G. , Sen Ganes C. 
Published: March 2005

ABSTRACTSendai virus (SeV) infection causes the transcriptional induction of many cellular genes that are also induced by interferon (IFN) or double-stranded RNA (dsRNA). We took advantage of various mutant cell lines to investigate the putative roles of the components of the IFN and dsRNA signaling pathways in the induction of those genes by SeV. Profiling the patterns of gene expression in SeV-infected cells demonstrated that Toll-like receptor 3, although essential for gene induction by dsRNA, was dispensable for gene induction by SeV. In contrast, Jak1, which mediates IFN signaling, was required for the induction of a small subset of genes by SeV. NF-κB and interferon regulatory factor 3 (IRF-3), the two major transcription factors activated by virus infection, were essential for the induction of two sets of genes by SeV. As expected, some of the IRF-3-dependent genes, such as ISG56, were more strongly induced by SeV in IRF-3-overexpressing cells. Surprisingly, in those cells, a number of NF-κB-dependent genes, such as the A20 gene, were induced poorly. Using a series of cell lines expressing increasing levels of IRF-3, we demonstrated that the degree of induction of A20 mRNA, upon SeV infection, was inversely proportional to the cellular level of IRF-3, whereas that of ISG56 mRNA was directly proportional. Thus, IRF-3 can suppress the expression of NF-κB-dependent genes in SeV-infected cells.
Retrovirus Restriction by TRIM5α Variants from Old World and New World Primates
Authors: Song Byeongwoon , Javanbakht Hassan , Perron Michel , Park Do Hyun , Stremlau Matthew , Sodroski Joseph 
Published: March 2005

ABSTRACT

The TRIM5α proteins of humans and some Old World monkeys have been shown to block infection of particular retroviruses following virus entry into the host cell. Infection of most New World monkey cells by the simian immunodeficiency virus of macaques (SIV
mac
) is restricted at a similar point. Here we examine the antiretroviral activity of TRIM5α orthologs from humans, apes, Old World monkeys, and New World monkeys. Chimpanzee and orangutan TRIM5α proteins functionally resembled human TRIM5α, potently restricting infection by N-tropic murine leukemia virus (N-MLV) and moderately restricting human immunodeficiency virus type 1 (HIV-1) infection. Notably, TRIM5α proteins from several New World monkey species restricted infection by SIV
mac
and the SIV of African green monkeys, SIV
agm
. Spider monkey TRIM5α, which has an expanded B30.2 domain v3 region due to a tandem triplication, potently blocked infection by a range of retroviruses, including SIV
mac
, SIV
agm
, HIV-1, and N-MLV. Tandem duplications in the TRIM5α B30.2 domain v1 region of African green monkeys are also associated with broader antiretroviral activity. Thus, variation in TRIM5α proteins among primate species accounts for the observed patterns of postentry restrictions in cells from these animals. The TRIM5α proteins of some monkey species exhibit dramatic lengthening of particular B30.2 variable regions and an expanded range of susceptible retroviruses.
ICP27 Recruits Aly/REF but Not TAP/NXF1 to Herpes Simplex Virus Type 1 Transcription Sites although TAP/NXF1 Is Required for ICP27 Export
Authors: Chen I-Hsiung Brandon , Li Ling , Silva Lindsey , Sandri-Goldin Rozanne M. 
Published: March 2005

ABSTRACT
Herpes simplex virus type 1 (HSV-1) protein ICP27 interacts with the cellular export adaptor protein Aly/REF, which is part of the exon junction complex implicated in cellular mRNA export. We previously reported that Aly/REF was no longer associated with splicing factor SC35 sites during infection but instead colocalized with ICP27 in distinct structures. Here we show that these structures colocalize with ICP4 and are sites of HSV-1 transcription. ICP27 mutants with lesions in the region required for the interaction with Aly/REF failed to recruit Aly/REF to viral transcription sites; however, ICP27 export to the cytoplasm was unimpaired, indicating that the interaction of ICP27 with Aly/REF is not required for ICP27 shuttling. ICP27 has also been shown to interact with the cellular mRNA export receptor TAP/NXF1. We report that ICP27 interacts directly with TAP/NXF1 and does not require Aly/REF to bridge the interaction. The C terminus of ICP27 is required; however, the N-terminal leucine-rich region also contributes to the interaction of ICP27 with TAP/NXF1. In contrast to the results found for Aly/REF, mutants that failed to interact with TAP/NXF1 were not exported to the cytoplasm, and TAP/NXF1 was not recruited to sites of HSV-1 transcription. Therefore, the interaction of ICP27 with TAP/NXF1 occurs after ICP27 leaves viral transcription sites. We conclude that ICP27 and the viral RNAs to which it binds are exported via the TAP/NXF1 export receptor.
Inhibition of RIG-I-Dependent Signaling to the Interferon Pathway during Hepatitis C Virus Expression and Restoration of Signaling by IKKε
Authors: Breiman Adrien , Grandvaux Nathalie , Lin Rongtuan , Ottone Catherine , Akira Shizuo , Yoneyama Mitsutoshi , Fujita Takashi , Hiscott John , Meurs Eliane F. 
Published: March 2005

ABSTRACT

Interferon (IFN) is one important effector of the innate immune response, induced by different viral or bacterial components through Toll-like receptor (TLR)-dependent and -independent mechanisms. As part of its pathogenic strategy, hepatitis C virus (HCV) interferes with the innate immune response and induction of IFN-β via the HCV NS3/4A protease activity which inhibits phosphorylation of IRF-3, a key transcriptional regulator of the IFN response. In the present study, we demonstrate that inhibition by the protease occurs upstream of the noncanonical IKK-related kinases IKKε and TBK-1, which phosphorylate IRF-3, through partial inhibition of the TLR adapter protein TRIF/TICAM1-dependent pathway. Use of TRIF
−/−
mouse embryo fibroblasts however revealed the presence of a TRIF-independent pathway involved in IFN induction that was also inhibited by NS3/4A. Importantly, we show that NS3/4A can strongly inhibit the ability of the recently described RIG-I protein to activate IFN, suggesting that RIG-I is a key factor in the TRIF-independent, NS3/4A-sensitive pathway. Expression of IFN signaling components including IKKε, TBK-1, TRIF, and wild type or constitutively active forms of RIG-I in the HCV replicon cells resulted in IFN-β promoter transactivation, with IKKε displaying the highest efficiency. Subsequently, overexpression of IKKε resulted in 80% inhibition of both the positive and negative replicative strands of the HCV replicon. The partial restoration of the capacity of the host cell to transcribe IFN-β indicates that IKKε expression is able to bypass the HCV-mediated inhibition and restore the innate antiviral response.
Expression of Glucose Transporter 1 Confers Susceptibility to Human T-Cell Leukemia Virus Envelope-Mediated Fusion
Authors: Coskun Ayse Kubra , Sutton Richard E. 
Published: March 2005

ABSTRACTHuman T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus identified and causes both adult T-cell leukemia/lymphoma and tropical spastic paraparesis/HTLV-1-associated myelopathy, among other disorders. In vitro, HTLV-1 has an extremely broad host cell tropism in that it is capable of infecting most mammalian cell types, although at the same time viral titers remain relatively low. Despite years of study, only recently has a bona fide candidate cellular receptor, glucose transporter 1 (glut-1), been identified. Although glut-1 was shown to bind specifically to the ectodomain of HTLV-1 and HTLV-2 envelope glycoproteins, which was reversible with small interfering RNA directed against glut-1, cellular susceptibility to HTLV upon expression of glut-1 was not established. Here we show that expression of glut-1 in relatively resistant MDBK cells conferred increased susceptibility to both HTLV-1- and HTLV-2-pseudotyped particles. glut-1 also markedly increased syncytium formation in MDBK cells after exposure to HTLV-1. Another assay also demonstrated HTLV-1 envelope-cell fusion in the presence of glut-1. Taken together, these results provide additional evidence that glut-1 is a receptor for HTLV.
Activation of CREB/ATF Sites by Polyomavirus Large T Antigen
Authors: Love Tara M. , de Jesus Rowena , Kean Jennifer A. , Sheng Qing , Leger Andrew , Schaffhausen Brian 
Published: March 2005

ABSTRACT
Polyomavirus large T antigen (LT) has a direct role in viral replication and a profound effect on cell phenotype. It promotes cell cycle progression, immortalizes primary cells, blocks differentiation, and causes apoptosis. While much of large T function is related to its effects on tumor suppressors of the retinoblastoma susceptibility (Rb) gene family, we have previously shown that activation of the cyclin A promoter can occur through a non-Rb-dependent mechanism. Here we show that activation occurs via an ATF/CREB site. Investigation of the mechanism indicates that large T can synergize with CREB family members to activate transcription. Experiments with Gal4-CREB constructs show that synergy is independent of CREB phosphorylation by protein kinase A. Examination of synergy with Gal4-CREB deletion constructs indicates that large T acts on the constitutive activation domain of CREB. Large T can bind to CREB in vivo. Genetic analysis shows that the DNA-binding domain (residues 264 to 420) is sufficient to activate transcription when it is localized to the nucleus. Further analysis of the DNA-binding domain shows that while site-specific DNA binding is not required, non-site-specific DNA binding is important for the activation. Thus, CREB binding and DNA binding are both important for large T activation of CREB/ATF sites. In contrast to previous models where large T transactivation occurred indirectly, these results also suggest that large T can act directly at promoters to activate transcription.
Human Papillomavirus Type 18 E6 Protein Binds the Cellular PDZ Protein TIP-2/GIPC, Which Is Involved in Transforming Growth Factor β Signaling and Triggers Its Degradation by the Proteasome
Authors: Favre-Bonvin Arnaud , Reynaud Caroline , Kretz-Remy Carole , Jalinot Pierre 
Published: March 2005

ABSTRACT

Several viral proteins expressed by DNA or RNA transforming viruses have the particular property of binding via their C-terminal end to various cellular proteins with PDZ domains. This study is focused on the PDZ protein TIP-2/GIPC, which was originally identified in two-hybrid screens performed with two different baits: the human T-cell leukemia virus type 1 Tax oncoprotein and the regulator of G signaling RGS-GAIP. Further studies have shown that TIP-2/GIPC is also able to associate with the cytoplasmic domains of various transmembrane proteins. In this report we show that TIP-2/GIPC interacts with the E6 protein of human papillomavirus type 18 (HPV-18). This event triggers polyubiquitination and proteasome-mediated degradation of the cellular protein. In agreement with this observation, silencing of E6 by RNA interference in HeLa cells causes an increase in the intracellular TIP-2/GIPC level. This PDZ protein has been previously found to be involved in transforming growth factor β (TGF-β) signaling by favoring expression of the TGF-β type III receptor at the cell membrane. In line with this activity of TIP-2/GIPC, we observed that depletion of this protein in HeLa cells hampers induction of the
Id3
gene by TGF-β treatment and also diminishes the antiproliferative effect of this cytokine. Conversely, silencing of E6 increases the expression of Id3 and blocks proliferation of HeLa cells. These results support the notion that HPV-18 E6 renders cells less sensitive to the cytostatic effect of TGF-β by lowering the intracellular amount of TIP-2/GIPC.