bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural, dielectric, and antimicrobial evaluation of PMMA/CeO2 for optoelectronic devices
Authors: Bakr Ahmed M. , Darwish Abdelfattah , Azab A. A. , El Awady Mohamed E. , Hamed Ahmed A. , Elzwawy Amir 
Published: January 2024

AbstractIn the current report, we have successfully synthesized nanocomposites of PMMA incorporating different doping of CeO2 through a chemical approach. XRD results reflects decent matching for CeO2 nanoparticles with 29 nm crystallite size. FTIR spectroscopy demonstrates the characteristic functional groups validating the successful formation of the composite. The optical study of PMMA and the nanocomposites has proven that the optical properties such as band gap, refractive index, optical permittivity, and loss tangent factor are affected by adding CeO2 to the PMMA matrix.The peak residing around 420 nm by UV measurements is allocated to occurring electrons photoexcitation from the valence to conduction band inherent in CeO2. The dielectric measurements were achieved using broadband dielectric spectroscopy upon a wide span of frequencies (10–1–107 Hz) and within temperatures from − 10 to 80 °C with a step of 10 °C. The permittivity decreases by adding CeO2 and the dielectric parameters are thermally enhanced, however, the temperature influence is based on CeO2 content, the higher the CeO2 amount, the higher the influence of temperature. The results of the nanocomposites revealed antibacterial activity counter to gram-positive bacteria strain (S. aureus, and B. subtilis), and gram-negative bacteria (E. coli, and K. pneumoniae), yeast (C. albicans, as well as fungi (A. niger). Inherently, the change in CeO2 concentration from 0.01 to 0.1 wt% delivers maximum influence against gram-negative bacteria. These PMMA CeO2-doped composites are beneficial for optoelectronic areas and devices.
Methods for controlling heavy metals in environmental soils based on artificial neural networks
Authors: Luo Ninglin 
Published: January 2024

AbstractThe problem of heavy metal pollution in soil has become a global environmental problem, and it is very important to predict and manage the heavy metals in the environmental soil in a timely manner. The changes in heavy metal content in soil have characteristics such as nonlinearity and large delay, making it difficult to predict heavy metals in soil using traditional methods. Traditional prediction methods are complex and cumbersome, which can lead to longer treatment time and easy secondary pollution. This article analyzed the Back Propagation neural network (BPNN) in artificial neural networks (ANN) and applied it to the prediction of heavy metals in environmental soils. BPNN has good nonlinear function approximation ability, so it can be well applied to complex problems such as soil heavy metal prediction. The methods of treating soil heavy metals include physical repair method, chemical repair method, microbial repair method, plant repair method, plant microbial combined repair method and so on. The use of BPNN can predict heavy metals in environmental soils through adaptive dynamic learning. However, the training time of the BPNN is relatively long and the convergence speed is relatively slow. Therefore, additional momentum terms were added to adjust the weights and thresholds of the network to improve the BPNN. In the experiment, the prediction performance of the improved BPNN was compared before and after the improvement. This article took 50 monitoring data of heavy metals in the same soil in a certain region in 2021 as sample data and predicted the content of heavy metals in the soil using improved and improved BPNN. Due to time constraints, this article only conducted experimental analysis on heavy metals such as lead and cadmium. In the first experiment, when the soil sample data was 50, the prediction accuracy of the BPNN for cadmium before and after improvement was 75.95% and 89.56%, respectively. In the second experiment, when the soil sample data was 50, the prediction accuracy of the BPNN for cadmium before and after improvement was 77.99% and 89.85%, respectively. The improved BPNN has good prediction accuracy and can effectively predict the status of heavy metals in soil. The analysis in this article can provide scientific basis for the comprehensive prevention and control of heavy metals in regional soil, and also provide reference for the development of pollution-free agriculture and ensuring food safety.
Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa
Authors: Daude Matheus Martins , Ságio Solange Aparecida , Rodrigues Jovielly Neves , Lima Nívea Maria Pereira , Lima André Almeida , Sarmento Maíra Ignacio , Sarmento Renato Almeida , Barreto Horllys Gomes 
Published: January 2024

AbstractRelative gene expression analysis through RT-qPCR is an important molecular technique that helps understanding different molecular mechanisms, such as the plant defense response to insect pests. However, the use of RT-qPCR for gene expression analysis can be affected by factors that directly affect the reliability of the results. Among these factors, the appropriate choice of reference genes is crucial and can strongly impact RT-qPCR relative gene expression analyses, highlighting the importance in correctly choosing the most suitable genes for the success of the analysis. Thus, this study aimed to select and validate reference genes for relative gene expression studies through RT-qPCR in hybrids of Eucalyptus tereticornis × Eucalyptus camaldulensis (drought tolerant and susceptible to Leptocybe invasa) under conditions of inoculation by the Beauveria bassiana fungus and subsequent infestation by L. invasa. The expression level and stability of eleven candidate genes were evaluated. Stability was analyzed using the RefFinder tool, which integrates the geNorm, NormFinder, BestKeeper, and Delta-Ct algorithms. The selected reference genes were validated through the expression analysis of the transcriptional factor EcDREB2 (dehydration-responsive element-binding protein 2). For all treatments evaluated, EcPTB, EcPP2A-1, and EcEUC12 were the best reference genes. The triplets EcPTB/EcEUC12/EcUBP6, EcPP2A-1/EcEUC12/EcPTB, EcIDH/EcSAND/Ecα-TUB, EcPP2A-1/Ecα-TUB/EcPTB, and EcPP2A-1/EcUPL7/EcSAND were the best reference genes for the control plants, mother plants, plants inoculated with B. bassiana, plants infested with L. invasa, and plants inoculated with B. bassiana and subsequently infested with L. invasa, respectively. The best determined reference genes were used to normalize the RT-qPCR expression data for each experimental condition evaluated. The results emphasize the importance of this type of study to ensure the reliability of relative gene expression analyses. Furthermore, the findings of this study can be used as a basis for future research, comprising gene expression analysis of different eucalyptus metabolic pathways.
Influence of copper(I) nicotinate complex on the Notch1 signaling pathway in triple negative breast cancer cell lines
Authors: Abdel-Mohsen Mohamed A. , Badawy Asmaa M. , Abu-Youssef Morsy A. , Yehia Mona A. , Abou Shamaa Lobna D. , Mohamed Shymaa Abdullah 
Published: January 2024

AbstractTriple negative breast cancer (TNBC) is a subtype of breast cancer which is characterized by its aggressiveness, poor and short overall survival. In this concept, there is a growing demand for metal-based compounds in TNBC therapy as copper complex that have a less toxic effect on normal cells and could stimulate apoptotic cell death. Additionally, Notch1 signaling pathway has received great attention as one of the most important potential targets for developing a novel therapeutic strategy. The present study is an attempt to assess the promising chemotherapeutic activities of copper(I) nicotinate (CNC) through its impact on the expression of downstream genes of Notch1 signaling pathway and the cell fate of TNBC. The co-treatment of TNBC cells with doxorubicin (Doxo) and CNC was also investigated. To approach the objective of the present study, TNBC cell lines; HCC1806 and MDAMB231, were utilized. MTT assay was used to determine the IC50 values of CNC and Doxo. After treatment, microtubule-associated protein light chain3 (LC3) were determined by flow cytometry. Additionally, qRT-PCR technique was used to detect the changes in genes levels that are involved Notch1 signaling pathway. Moreover, autophagosomes were monitored and imaged by Transmission electron microscopy. Treatment of TNBC cells with CNC modulated Notch1 signaling pathway in different manners with respect to the type of cells and the applied dose of CNC. The observed effects of CNC may reflect the possible anti-cancer activities of CNC in both types of TNBC. However, cell type and CNC dose should be considered.
Rat microbial biogeography and age-dependent lactic acid bacteria in healthy lungs
Authors: Zhao Lan , Cunningham Christine M. , Andruska Adam M. , Schimmel Katharina , Ali Md Khadem , Kim Dongeon , Gu Shenbiao , Chang Jason L. , Spiekerkoetter Edda , Nicolls Mark R. 
Published: January 2024

AbstractThe laboratory rat emerges as a useful tool for studying the interaction between the host and its microbiome. To advance principles relevant to the human microbiome, we systematically investigated and defined the multitissue microbial biogeography of healthy Fischer 344 rats across their lifespan. Microbial community profiling data were extracted and integrated with host transcriptomic data from the Sequencing Quality Control consortium. Unsupervised machine learning, correlation, taxonomic diversity and abundance analyses were performed to determine and characterize the rat microbial biogeography and identify four intertissue microbial heterogeneity patterns (P1–P4). We found that the 11 body habitats harbored a greater diversity of microbes than previously suspected. Lactic acid bacteria (LAB) abundance progressively declined in lungs from breastfed newborn to adolescence/adult, and was below detectable levels in elderly rats. Bioinformatics analyses indicate that the abundance of LAB may be modulated by the lung–immune axis. The presence and levels of LAB in lungs were further evaluated by PCR in two validation datasets. The lung, testes, thymus, kidney, adrenal and muscle niches were found to have age-dependent alterations in microbial abundance. The 357 microbial signatures were positively correlated with host genes in cell proliferation (P1), DNA damage repair (P2) and DNA transcription (P3). Our study established a link between the metabolic properties of LAB with lung microbiota maturation and development. Breastfeeding and environmental exposure influence microbiome composition and host health and longevity. The inferred rat microbial biogeography and pattern-specific microbial signatures could be useful for microbiome therapeutic approaches to human health and life quality enhancement.
PTEN pathogenic variants are associated with poor prognosis in patients with advanced soft tissue sarcoma
Authors: Pan Minggui , Zhou Maggie Y. , Jiang Chen , Zhang Zheyang , Bui Nam , Bien Jeffrey , Siy Amanda , Achacoso Ninah , Solorzano Aleyda V. , Tse Pam , Chung Elaine , Hu Wenwei , Thomas Sachdev , Ganjoo Kristen , Habel Laurel A. 
Published: January 2024

Abstract
Background
We aimed to examine whether PTEN pathogenic variants (mutPTEN) were associated with overall survival (OS) in patients with advanced soft tissue sarcoma (STS) with the presence of one or more of the most common genomic alterations including p53, CDKN2A, RB1, and ATRX pathogenic variants.

Methods
This study included patients from Kaiser Permanente Northern California and Stanford Cancer Center with grade 2 or higher locally advanced and metastatic STS.

Results
A total of 174 patients had leiomyosarcoma (LMS), 136 had undifferentiated pleomorphic sarcoma (UPS), 78 had Liposarcoma (LPS), and 214 had other histology subtypes (Others). Among all patients with STS, OS was worse for those with mutPTEN versus wild-type PTEN (wtPTEN, adjusted HR [aHR] = 1.58 [95% CI, 1.11–2.23]), mutCDKN2A vs wtCDKN2A (aHR = 1.33 [95% CI .99–1.80]), and mutRB1 vs wtRB1 (aHR = 1.26 [95% CI 0.93–1.70[), while OS was similar for mutp53 vs wtp53 and mutATRX vs wtATRX. MutPTEN versus wtPTEN was consistently associated with worse OS in histologic subtypes including LMS and UPS and molecular subgroups.

Conclusion
MutPTEN vs wtPTEN was associated with worse OS in advanced STS. If confirmed, our findings could be helpful for prognostic stratification in clinical practice and for further understanding the molecular mechanisms of STS.
Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria
Authors: Fraering Jeremy , Salnot Virginie , Gautier Emilie-Fleur , Ezinmegnon Sem , Argy Nicolas , Peoc’h Katell , Manceau Hana , Alao Jules , Guillonneau François , Migot-Nabias Florence , Bertin Gwladys I , Kamaliddin Claire ,   NeuroCM consortium, Aubouy Agnes , Affolabi Dissou , Biokou Bibiane , Cot Michel , Degbelo Jean-Eudes , Deloron Philippe , Dramane Latifou , Faucher Jean-François , Guillochon Emilie , Houze Ludivine , Jafari-Guemouri Sayeh , Houze Sandrine , Joste Valentin , Labrunie Anaïs , Ladipo Yélé , Lathiere Thomas , Massougbodji Achille , Mowendabeka Audrey , Papin Jade , Pipy Bernard , Preux Pierre-Marie , Raymondeau Marie , Royo Jade , Sossou Darius , Techer Brigitte , Vianou Bertin 
Published: January 2024

AbstractCerebral malaria (CM), the most lethal complication of Plasmodium falciparum severe malaria (SM), remains fatal for 15–25% of affected children despite the availability of treatment. P. falciparum infects and multiplies in erythrocytes, contributing to anemia, parasite sequestration, and inflammation. An unbiased proteomic assessment of infected erythrocytes and plasma samples from 24 Beninese children was performed to study the complex mechanisms underlying CM. A significant down-regulation of proteins from the ubiquitin–proteasome pathway and an up-regulation of the erythroid precursor marker transferrin receptor protein 1 (TFRC) were associated with infected erythrocytes from CM patients. At the plasma level, the samples clustered according to clinical presentation. Significantly, increased levels of the 20S proteasome components were associated with SM. Targeted quantification assays confirmed these findings on a larger cohort (n = 340). These findings suggest that parasites causing CM preferentially infect reticulocytes or erythroblasts and alter their maturation. Importantly, the host plasma proteome serves as a specific signature of SM and presents a remarkable opportunity for developing innovative diagnostic and prognostic biomarkers.
A two-electron reducing reaction of CO2 to an oxalate anion: a theoretical study of delocalized (presolvated) electrons in Al(CH3)n(NH3)m, n = 0–2 and m = 1–6, clusters
Authors: Alikhani Mohammad Esmail , Janesko Benjamin G. 
Published: January 2024

A C–C coupling takes place when two CO2 molecules interact with the Al2(CH3)2(CH2)2(NH3)4 solvated electron precursor leading to the formation of an oxalate.
Massive black holes in nuclear star clusters
Authors: Hoyer N. , Arcodia R. , Bonoli S. , Merloni A. , Neumayer N. , Zhang Y. , Comparat J. 
Published: January 2024

Context. Massive black holes (MBHs) are typically hosted in the centres of massive galaxies but they appear to become rarer in lower mass galaxies, where nuclear star clusters (NSCs) frequently appear instead. The transition region, where both an MBH and NSC can co-exist, has been poorly studied to date and only a few dozen galaxies are known to host them. One avenue for detecting new galaxies with both an MBH and NSC is to look for accretion signatures of MBHs.Aims. Here, we use new SRG/eROSITA all-sky survey eRASS:4 data to search for X-ray signatures of accreting MBHs in NSCs, while also investigating their combined occupation fraction.Methods. We collected more than 200 galaxies containing an NSC, spanning multiple orders in terms of galaxy stellar mass and morphological type, within the footprint of the German eROSITA Consortium survey. We determined the expected X-ray contamination from binary stellar systems using the galaxy stellar mass and star formation rate as estimated from far-ultraviolet and mid-infrared emission.Results. We find significant detections for 18 galaxies (~8.3%), including one ultra-luminous X-ray source; however, only three galaxies (NGC 2903, 4212, and 4639) have X-ray luminosities that are higher than the expected value from X-ray binaries, indicative of the presence of an MBH. In addition, the X-ray luminosity of six galaxies (NGC 2903, 3384, 4321, 4365, 4639, and 4701) differs from previous studies and could indicate the presence of a variable active galactic nucleus. For NGC 4701 specifically, we find a variation of X-ray flux within the eRASS:4 data set. Stacking X-ray non-detected galaxies in the dwarf regimeM*gal≤ 109M⊙) results in luminosity upper limits of a few times 1038erg s−1. The combined occupation fraction of accreting MBHs and NSCs becomes non-zero for galaxy masses above ~ 107.5M⊙and this result is slightly elevated as compared to the literature data.Conclusions. Our data extend, for the first time, towards the dwarf elliptical galaxy regime and identify promising MBH candidates for higher resolution follow-up observations. At most galaxy masses (and with the exception of three cases), the X-ray constraints are consistent with the expected emission from binary systems or an Eddington fraction of at most 0.01%, assuming a black holes mass of 106.5M⊙. This work confirms the known complexities in similar-type of studies, while providing the appealing alternative of using X-ray survey data of in-depth observations of individual targets with higher resolution instruments.
Mapping thermal emission in the synchrotron-dominated supernova remnants G330.2+1.0, 3C58, and RX J1713.7-3946
Authors: Picquenot A. , Williams B. J. , Acero F. , Mori K. 
Published: January 2024

Aims. Since the discovery of synchrotron X-ray emission from the shell of the supernova remnant (SNR) SN 1006, multiple observations from Chandra and XMM-Newton have shown that many young SNRs produce synchrotron emission in X-rays. Among those, a few peculiar SNRs have their X-ray emission largely dominated by synchrotron radiation, showing no or only faint traces of thermal emission. In this paper, we report our mapping of the thermal emission in three emblematic synchrotron-dominated SNRs: G330.2+1.0, 3C58, and RX J1713.7-3946.
Methods. We used a blind source separation method able to retrieve faint components from X-ray data in the form of Chandra and XMM-Newton observations. The thermal candidates disentangled by the algorithm were then used to select regions of extraction. We then analyzed the extracted spectra to assess their physical nature.
Results. We conclude that the components retrieved by the algorithm indeed represent the spatial distribution of the thermal emission in G330.2+1.0 and 3C58, and a likely thermal candidate in RX J1713.7-3946. Our findings confirm and expand on past studies.
The SRG/eROSITA all-sky survey
Authors: Freund S. , Czesla S. , Predehl P. , Robrade J. , Salvato M. , Schneider P. C. , Starck H. , Wolf J. , Schmitt J. H. M. M. 
Published: January 2024

Context. The first eROSITA all-sky survey (eRASS1) performed on board the Spectrum-Roentgen-Gamma mission (SRG) provides more than 900 000 X-ray sources in the 0.2–2.3 keV band located in the western hemisphere.
Aims. We present identifications of the eRASS1 sources obtained using our HamStar method, which was designed for the identification of coronal X-ray sources.
Methods. HamStar is a Bayesian framework that estimates coronal probabilities for each eRASS1 source based on a cross-match with optical counterparts from Gaia DR3. It considers geometric properties, such as angular separation and positional uncertainty, as well the additional properties of fractional X-ray flux, color, and distance.
Results. We identify 138 800 coronal eRASS1 sources and estimate a completeness and reliability of about 91.5% for this sample, which we confirmed with Chandra detections. This is the largest available sample of coronal X-ray emitters and we find nearly five times as many coronal sources as in the ROSAT all-sky survey. The coronal eRASS1 sources are made up of all spectral types and the onset of convection and the saturation limit are clearly visible. As opposed to previous samples, rare source types are also well populated. About 10% of the coronal eRASS1 sources have a correlated secondary counterpart, which is a wide binary companion or belongs to the same stellar cluster. We also identify 6700 known unresolved binaries, and an excess of fast binary periods below 10 d. Furthermore, the binary sequence is clearly visible in a color–magnitude diagram. When combining the coronal eRASS1 sources with rotation modulations from Gaia DR3, we find 3700 X-ray sources with known rotation periods, which is the largest sample of this kind. We fitted the rotation–activity relation and convection turnover times for our flux-limited sample. We do not detect the low-amplitude fast rotators discovered in the Gaia DR3 sample in X-rays.
Mid-gastric peroral endoscopic myotomy in the treatment of post-sleeve gastrectomy gastric tube twist: Four cases
Authors: Mugnier Angelique , Vitton Veronique , Barthet Marc , Gonzalez Jean-Michel 
Published: January 2024

Abstract
Background and study aims Laparoscopic sleeve gastrectomy (LSG) may lead to symptomatic mid-gastric twist in 0.1% to 4% of cases. Endoscopic balloon dilatation often fails, requiring a switch to Roux-en-Y bypass. Submucosal endoscopic treatment is expanding, so we propose a new technique of fibrosis incision after tunnelization, called medio-gastric peroral endoscopic myotomy (MG-POEM).
Patients and methods Four patients aged 41 to 70 years underwent MG-POEM in 2020 for symptomatic post-LSG medio-gastric twist. The aim was to describe the technique, and document the efficacy, using the Gastric Outlet Obstruction Scoring System (GOOSS) score and a quality-of-life scale (QOLS). The procedures consisted of a POEM with myotomy and fibrosis incision.
Results Median preoperative GOOSS and QOLS were 1/3 ([0–3] and 1.75/10 [0–10], respectively. All procedures were technically successful. One patient had immediate postoperative complications, which were treated conservatively. The median follow-up was 18 months. All patients had improved quality of life and food intake, with median postoperative GOOSS and QOLS of 3/3 and 6,5/10 [6–8], respectively. Two patients required additional pneumatic dilatation for scarring strictures. Follow-up endoscopies and esophagograms showed that the twist had disappeared.
Conclusions MG-POEM shows promise for safe and effective management of post-LSG mid-gastric twist, despite requiring a high level of endoscopic skill to perform submucosal tunneling.