bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drug memory reconsolidation: from molecular mechanisms to the clinical context
Authors: Milton Amy L. 
Published: December 2023

AbstractSince its rediscovery at the beginning of the 21st Century, memory reconsolidation has been proposed to be a therapeutic target for reducing the impact of emotional memories that can go awry in mental health disorders such as drug addiction (substance use disorder, SUD). Addiction can be conceptualised as a disorder of learning and memory, in which both pavlovian and instrumental learning systems become hijacked into supporting drug-seeking and drug-taking behaviours. The past two decades of research have characterised the details of the molecular pathways supporting the reconsolidation of pavlovian cue-drug memories, with more recent work indicating that the reconsolidation of instrumental drug-seeking memories also relies upon similar mechanisms. This narrative review considers what is known about the mechanisms underlying the reconsolidation of pavlovian and instrumental memories associated with drug use, how these approaches have translated to experimental medicine studies, and the challenges and opportunities for the clinical use of reconsolidation-based therapies.
Suicidality and its associated factors among mood disorder patients in emergency department in China: a comparative study using propensity score matching approach
Authors: Xie Xiao-meng , Wang Yi-Fan , Han Tian , Liu Yi , Li Juan , Zhu Hui , Jiang Tao , Ji Xiao , Cai Hong 
Published: December 2023

AbstractSuicidality in mood disorder patients is common, especially in emergency department (ED), but the patterns and associated factors of suicidality are not clear. This study compared biomarkers and mental health symptoms (i.e., depression, anxiety, and psychiatric symptoms) between mood disorder patients with and without the whole range of suicidality comprising suicidal ideation (SI), suicide plan (SP), and suicide attempt (SA). This cross-sectional, comparative, convenient-sampling study was conducted between January 2021 and March 2022, in emergency department of Beijing Anding Hospital, China. Patients with mood disorders at a psychiatric emergency department were assessed, with measurements of suicidality, biomarkers, depressive, anxiety, and psychiatric symptoms were assessed using the 24 items-Hamilton Depression Rating Scale (HAMD-24), Hamilton Anxiety Rating Scale (HAMA), Young Manic Rating Scale (YMRS) and Brief Psychiatric Rating Scale (BPRS), respectively. The propensity score matching (PSM) method was used to identify patients in mood disorder with and without SI, SP, and SA. A generalized linear model (GLM) was used to assess the differences in biomarkers, depressive, anxiety, and psychiatric symptoms between patients in mood disorder with and without SI, SP, and SA. In total, 898 participated in this survey and completed the assessment. Illness duration was significantly negatively associated with SA (OR = 0.969, 95%CI = 0.939–0.999, P = 0.046). HAMD-24 total score was significantly positively associated with the SI (OR = 1.167, 95%CI = 1.134–1.201, p < 0.001), SP (OR = 1.159, 95%CI = 1.126–1.192, p < 0.001) and SA (OR = 1.189, 95%CI = 1.144–1.235, p < 0.001) of the matched samptched sample. However, YMRS total score was significantly negatively associated with the SI (OR = 0.928, 95%CI = 0.905–0.951, p < 0.001), SP (OR = 0.920, 95%CI = 0.897–0.944, p < 0.001) and SA (OR = 0.914, 95%CI = 0.890–0.938, p < 0.001) of the matched sample after adjusting for age, gender, marital status, and occupation. The duration of illness, severity of depressive symptoms and severity of manic symptoms appeared to be more likely to influence suicidality. Considering the significant risk of suicide in mood disorders on psychiatric emergency care, timely treatment and effective management of suicidality in this population group need to be developed.
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry: Proposal for a bone marrow quality index
Authors: Óskarsson Jón Þórir , Rögnvaldsson Sæmundur , Thorsteinsdottir Sigrun , Aspelund Thor , Gunnarsson Steinar Bragi , Hákonardóttir Guðlaug Katrín , Sigurðardóttir Guðrún Ásta , Þórðardóttir Ásdís Rósa , Gíslason Gauti Kjartan , Ólafsson Andri , Sigurðsson Jón Kristinn , Eyþórsson Elías , Jónsson Ásbjörn , Viðarsson Brynjar , Önundarson Páll Torfi , Agnarsson Bjarni A. , Pálmason Róbert , Sigurðardóttir Margrét , Þorsteinsdóttir Ingunn , Ólafsson Ísleifur , Harding Stephen , Flores-Montero Juan , Orfao Alberto , Durie Brian G. M. , Love Thorvardur Jon , Kristinsson Sigurdur Yngvi 
Published: December 2023

AbstractHemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-associated cell populations, assessed by next-generation flow cytometry (NGF) and white blood cell (WBC) count in 351 BM aspirated samples from 219 participants with plasma cell disorders in the Iceland Screens, Treats, or Prevents MM study (iStopMM), as markers of hemodilution by their discriminatory ability between first and (generally more hemodiluted) second pull BM aspirated samples. The most discriminating markers were used to derive a novel BM quality index (BMQI). Nucleated red blood cells and myeloid precursors provided the greatest discriminatory ability between first vs second pull samples (area under the curve (AUC): 0.87 and 0.85, respectively), significantly better than B cell precursors (AUC = 0.64;p < 0.001), mast cells (AUC = 0.65;p < 0.001), and the BM WBC count (AUC = 0.77;p < 0.05). We generated a novel BMQI that is intrinsic to current NGF protocols, for evaluating quality of diagnostic BM samples and suggest the use of a BMQI scoring system for interpreting results and guiding appropriate actions.
Ferroptosis in epithelial ovarian cancer: a burgeoning target with extraordinary therapeutic potential
Authors: Ruan Danhua , Wen Jirui , Fang Fei , Lei Yuqin , Zhao Zhiwei , Miao Yali 
Published: December 2023

AbstractEpithelial ovarian cancer (EOC) is universally acknowledged as a terrifying women killer for its high mortality. Recent research advances support that ferroptosis, an emerging iron-dependent type of regulated cell death (RCD) triggered by the excessive accumulation of lipid peroxides probably possesses extraordinary therapeutic potential in EOC therapy. Herein, we firstly provide a very concise introduction of ferroptosis. Special emphasis will be put on the ferroptosis’s vital role in EOC, primarily covering its role in tumorigenesis and progression of EOC, the capability of reversing chemotherapy resistance, and the research and development of related therapeutic strategies. Furthermore, the construction of ferroptosis-related prognostic prediction systems, and mechanisms of ferroptosis resistance in EOC are also discussed. Finally, we propose and highlight several important yet unanswered problems and some future research directions in this field.
BaZrO3/MgO-templated epitaxy showing a conductivity increase of three orders of magnitude for the Ba0.95La0.05SnO3 films on Al2O3 substrates, with very high transparency and X-band electromagnetic shielding
Authors: Ha Youngkyoung , Jeon Jingyeong , Hwang Subhin , MacManus-Driscoll Judith L. , Lee Shinbuhm 
Published: December 2023

AbstractTransparent conductors with electromagnetic shielding capabilities (TC-EMS) are rare, despite their significant potential for creating new functionalities in energy and military applications. Here, we investigate the potential of La-doped BaSnO3 (BLSO) for TC-EMS since its epitaxial film has been known to have low sheet resistance and high visible transmittance. However, films grown on industrially practical Al2O3 substrates exhibit a sheet resistance three orders of magnitude higher than that of reported films grown on perovskites. Here, this problem is addressed by templating a BaZrO3/MgO bilayer on (0001)-oriented Al2O3 substrates to yield single-crystalline BLSO epitaxial films. The absence of grain boundaries in the epitaxial films minimizes the electron scattering. Due to the affirmative correlation between the conductivity and crystallinity, 5% La doping is optimal among the 5−20% La concentrations studied; these 480-nm-thick films have the highest crystallinity and the lowest sheet resistances of ~28 Ω ▯−1; this value is similar to that of single-crystalline levels. Due to their very high transmittances (~82% in a range 400−1000 nm) and effective X-band electromagnetic shielding (~18.6 dB), the BLSO epitaxial films grown on Al2O3 have great potential to be used for inexpensive TC-EMS applications.
Defining a core configuration for human centromeres during mitosis
Authors: Sen Gupta Ayantika , Seidel Chris , Tsuchiya Dai , McKinney Sean , Yu Zulin , Smith Sarah E. , Unruh Jay R. , Gerton Jennifer L. 
Published: December 2023

AbstractThe centromere components cohesin, CENP-A, and centromeric DNA are essential for biorientation of sister chromatids on the mitotic spindle and accurate sister chromatid segregation. Insight into the 3D organization of centromere components would help resolve how centromeres function on the mitotic spindle. We use ChIP-seq and super-resolution microscopy with single particle averaging to examine the geometry of essential centromeric components on human chromosomes. Both modalities suggest cohesin is enriched at pericentromeric DNA. CENP-A localizes to a subset of the α-satellite DNA, with clusters separated by ~562 nm and a perpendicular intervening ~190 nM wide axis of cohesin in metaphase chromosomes. Differently sized α-satellite arrays achieve a similar core structure. Here we present a working model for a common core configuration of essential centromeric components that includes CENP-A nucleosomes, α-satellite DNA and pericentromeric cohesion. This configuration helps reconcile how centromeres function and serves as a foundation to add components of the chromosome segregation machinery.
A fungal plant pathogen discovered in the Devonian Rhynie Chert
Authors: Strullu-Derrien Christine , Goral Tomasz , Spencer Alan R. T. , Kenrick Paul , Catherine Aime M. , Gaya Ester , Hawksworth David L. 
Published: December 2023

AbstractFungiare integral to well-functioning ecosystems, and their broader impact on Earth systems is widely acknowledged. Fossil evidence from the Rhynie Chert (Scotland, UK) shows thatFungiwere already diverse in terrestrial ecosystems over 407-million-years-ago, yet evidence for the occurrence ofDikarya (the subkingdom ofFungithat includes the phylaAscomycotaandBasidiomycota) in this site is scant. Here we describe a particularly well-preserved asexual fungus from the Rhynie Chert which we examined using brightfield and confocal microscopy. We documentPotteromyces asteroxylicolagen. et sp. nov. that we attribute toAscomycota incertae sedis (Dikarya). The fungus forms a stroma-like structure with conidiophores arising in tufts outside the cuticle on aerial axes and leaf-like appendages of the lycopsid plantAsteroxylon mackiei. It causes a reaction in the plant that gives rise to dome-shaped surface projections. This suite of features in the fungus together with the plant reaction tissues provides evidence of it being a plant pathogenic fungus. The fungus evidently belongs to an extinct lineage of ascomycetes that could serve as a minimum node age calibration point for theAscomycotaas a whole, or even theDikaryacrown group, along with some otherAscomycotapreviously documented in the Rhynie Chert.
Intrinsic factors and CD1d1 but not CD1d2 expression levels control invariant natural killer T cell subset differentiation
Authors: Amable Ludivine , Ferreira Martins Luis Antonio , Pierre Remi , Do Cruseiro Marcio , Chabab Ghita , Sergé Arnauld , Kergaravat Camille , Delord Marc , Viret Christophe , Jaubert Jean , Liu Chaohong , Karray Saoussen , Marie Julien C. , Irla Magali , Georgiev Hristo , Clave Emmanuel , Toubert Antoine , Lucas Bruno , Klibi Jihene , Benlagha Kamel 
Published: December 2023

AbstractInvariant natural killer T (NKT) cell subsets are defined based on their cytokine-production profiles and transcription factors. Their distribution is different in C57BL/6 (B6) and BALB/c mice, with a bias for NKT1 and NKT2/NKT17 subsets, respectively. Here, we show that the non-classical class I-like major histocompatibility complex CD1 molecules CD1d2, expressed in BALB/c and not in B6 mice, could not account for this difference. We find however that NKT cell subset distribution is intrinsic to bone marrow derived NKT cells, regardless of syngeneic CD1d-ligand recognition, and that multiple intrinsic factors are likely involved. Finally, we find that CD1d expression levels in combination with T cell antigen receptor signal strength could also influence NKT cell distribution and function. Overall, this study indicates that CD1d-mediated TCR signals and other intrinsic signals integrate to influence strain-specific NKT cell differentiation programs and subset distributions.
Emergence of the cortical encoding of phonetic features in the first year of life
Authors: Di Liberto Giovanni M. , Attaheri Adam , Cantisani Giorgia , Reilly Richard B. , Ní Choisdealbha Áine , Rocha Sinead , Brusini Perrine , Goswami Usha 
Published: December 2023

AbstractEven prior to producing their first words, infants are developing a sophisticated speech processing system, with robust word recognition present by 4–6 months of age. These emergent linguistic skills, observed with behavioural investigations, are likely to rely on increasingly sophisticated neural underpinnings. The infant brain is known to robustly track the speech envelope, however previous cortical tracking studies were unable to demonstrate the presence of phonetic feature encoding. Here we utilise temporal response functions computed from electrophysiological responses to nursery rhymes to investigate the cortical encoding of phonetic features in a longitudinal cohort of infants when aged 4, 7 and 11 months, as well as adults. The analyses reveal an increasingly detailed and acoustically invariant phonetic encoding emerging over the first year of life, providing neurophysiological evidence that the pre-verbal human cortex learns phonetic categories. By contrast, we found no credible evidence for age-related increases in cortical tracking of the acoustic spectrogram.
High-throughput ab initio design of atomic interfaces using InterMatch
Authors: Gerber Eli , Torrisi Steven B. , Shabani Sara , Seewald Eric , Pack Jordan , Hoffman Jennifer E. , Dean Cory R. , Pasupathy Abhay N. , Kim Eun-Ah 
Published: December 2023

AbstractForming a hetero-interface is a materials-design strategy that can access an astronomically large phase space. However, the immense phase space necessitates a high-throughput approach for an optimal interface design. Here we introduce a high-throughput computational framework, InterMatch, for efficiently predicting charge transfer, strain, and superlattice structure of an interface by leveraging the databases of individual bulk materials. Specifically, the algorithm reads in the lattice vectors, density of states, and the stiffness tensors for each material in their isolated form from the Materials Project. From these bulk properties, InterMatch estimates the interfacial properties. We benchmark InterMatch predictions for the charge transfer against experimental measurements and supercell density-functional theory calculations. We then use InterMatch to predict promising interface candidates for doping transition metal dichalcogenide MoSe2. Finally, we explain experimental observation of factor of 10 variation in the supercell periodicity within a few microns in graphene/α-RuCl3 by exploring low energy superlattice structures as a function of twist angle using InterMatch. We anticipate our open-source InterMatch algorithm accelerating and guiding ever-growing interfacial design efforts. Moreover, the interface database resulting from the InterMatch searches presented in this paper can be readily accessed online.
Structural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8
Authors: Sun Dawei , Sun Yonglian , Janezic Eric , Zhou Tricia , Johnson Matthew , Azumaya Caleigh , Noreng Sigrid , Chiu Cecilia , Seki Akiko , Arenzana Teresita L. , Nicoludis John M. , Shi Yongchang , Wang Baomei , Ho Hoangdung , Joshi Prajakta , Tam Christine , Payandeh Jian , Comps-Agrar Laëtitia , Wang Jianyong , Rutz Sascha , Koerber James T. , Masureel Matthieu 
Published: December 2023

AbstractThe C-C motif chemokine receptor 8 (CCR8) is a class A G-protein coupled receptor that has emerged as a promising therapeutic target in cancer. Targeting CCR8 with an antibody has appeared to be an attractive therapeutic approach, but the molecular basis for chemokine-mediated activation and antibody-mediated inhibition of CCR8 are not fully elucidated. Here, we obtain an antagonist antibody against human CCR8 and determine structures of CCR8 in complex with either the antibody or the endogenous agonist ligand CCL1. Our studies reveal characteristic antibody features allowing recognition of the CCR8 extracellular loops and CCL1-CCR8 interaction modes that are distinct from other chemokine receptor - ligand pairs. Informed by these structural insights, we demonstrate that CCL1 follows a two-step, two-site binding sequence to CCR8 and that antibody-mediated inhibition of CCL1 signaling can occur by preventing the second binding event. Together, our results provide a detailed structural and mechanistic framework of CCR8 activation and inhibition that expands our molecular understanding of chemokine - receptor interactions and offers insight into the development of therapeutic antibodies targeting chemokine GPCRs.
Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal
Authors: Shi Jian-Hui , Chen Yu-Xia , Feng Yingying , Yang Xiaohang , Lin Jie , Wang Ting , Wei Chun-Chun , Ma Xian-Hua , Yang Rui , Cao Dongmei , Zhang Hai , Xie Xiangyang , Xie Zhifang , Zhang Weiping J. 
Published: December 2023

AbstractArginase, a manganese (Mn)-dependent enzyme, is indispensable for urea generation and ammonia disposal in the liver. The potential role of fructose in Mn and ammonia metabolism is undefined. Here we demonstrate that fructose overconsumption impairs hepatic Mn homeostasis and ammonia disposal in male mice. Fructose overexposure reduces liver Mn content as well as its activity of arginase and Mn-SOD, and impairs the clearance of blood ammonia under liver dysfunction. Mechanistically, fructose activates the Mn exporter Slc30a10 gene transcription in the liver in a ChREBP-dependent manner. Hepatic overexpression of Slc30a10 can mimic the effect of fructose on liver Mn content and ammonia disposal. Hepatocyte-specific deletion of Slc30a10 or ChREBP increases liver Mn contents and arginase activity, and abolishes their responsiveness to fructose. Collectively, our data establish a role of fructose in hepatic Mn and ammonia metabolism through ChREBP/Slc30a10 pathway, and postulate fructose dietary restriction for the prevention and treatment of hyperammonemia.
Opposing brain signatures of sleep in task-based and resting-state conditions
Authors: Abdelhack Mohamed , Zhukovsky Peter , Milic Milos , Harita Shreyas , Wainberg Michael , Tripathy Shreejoy J. , Griffiths John D. , Hill Sean L. , Felsky Daniel 
Published: December 2023

AbstractSleep and depression have a complex, bidirectional relationship, with sleep-associated alterations in brain dynamics and structure impacting a range of symptoms and cognitive abilities. Previous work describing these relationships has provided an incomplete picture by investigating only one or two types of sleep measures, depression, or neuroimaging modalities in parallel. We analyze the correlations between brainwide neural signatures of sleep, cognition, and depression in task and resting-state data from over 30,000 individuals from the UK Biobank and Human Connectome Project. Neural signatures of insomnia and depression are negatively correlated with those of sleep duration measured by accelerometer in the task condition but positively correlated in the resting-state condition. Our results show that resting-state neural signatures of insomnia and depression resemble that of rested wakefulness. This is further supported by our finding of hypoconnectivity in task but hyperconnectivity in resting-state data in association with insomnia and depression. These observations dispute conventional assumptions about the neurofunctional manifestations of hyper- and hypo-somnia, and may explain inconsistent findings in the literature.
Nanoscale feedback control of six degrees of freedom of a near-sphere
Authors: Kamba Mitsuyoshi , Shimizu Ryoga , Aikawa Kiyotaka 
Published: December 2023

AbstractManipulating the rotational as well as the translational degrees of freedom of rigid bodies has been a crucial ingredient in diverse areas, from optically controlled micro-robots, navigation, and precision measurements at macroscale to artificial and biological Brownian motors at nanoscale. Here, we demonstrate feedback cooling of all the angular motions of a near-spherical neutral nanoparticle with all the translational motions feedback-cooled to near the ground state. The occupation numbers of the three translational motions are 6 ± 1, 6 ± 1, and 0.69 ± 0.18. A tight, anisotropic optical confinement allows us to clearly observe three angular oscillations and to identify the ratio of two radii to the longest radius with a precision of 0.08 %. We develop a thermometry for three angular oscillations and realize feedback cooling of them to temperatures of lower than 0.03 K by electrically controlling the electric dipole moment of the nanoparticle.
Structural basis of a redox-dependent conformational switch that regulates the stress kinase p38α
Authors: Pous Joan , Baginski Blazej , Martin-Malpartida Pau , González Lorena , Scarpa Margherita , Aragon Eric , Ruiz Lidia , Mees Rebeca A. , Iglesias-Fernández Javier , Orozco Modesto , Nebreda Angel R. , Macias Maria J. 
Published: December 2023

AbstractMany functional aspects of the protein kinase p38α have been illustrated by more than three hundred structures determined in the presence of reducing agents. These structures correspond to free forms and complexes with activators, substrates, and inhibitors. Here we report the conformation of an oxidized state with an intramolecular disulfide bond between Cys119 and Cys162 that is conserved in vertebrates. The structure of the oxidized state does not affect the conformation of the catalytic site, but alters the docking groove by partially unwinding and displacing the short αD helix due to the movement of Cys119 towards Cys162. The transition between oxidized and reduced conformations provides a mechanism for fine-tuning p38α activity as a function of redox conditions, beyond its activation loop phosphorylation. Moreover, the conformational equilibrium between these redox forms reveals an unexplored cleft for p38α inhibitor design that we describe in detail.