bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variability of the mitochondrial genome in young Yakut patients with breast cancer
Authors: Tikhonov D. G. , Egorov A. N. , Golubenko M. V. , Molokov A. Yu. , Belyavskaya V. A. , Gervas P. A. , Skryabin N. A. 
Published: January 2024

Background. The Sakha (Yakutia) population, the indigenous population of Siberia living in Yakutia, has one of the lowest rates of breast cancer (BC) incidence worldwide. The low BC incidence among the indigenous population of Yakutia has been reported by several authors, but to date the reasons for this phenomenon have not been fully elucidated. It should be noted that the study of factors that reduce the risk of BC is important for its prevention. In several studies, no hereditary BC was found in the Yakuts, and no pathogenic variants of the BRCA1/2 genes associated with hereditary syndromes of breast and ovarian cancers were found. In this regard, we decided to shift the focus to studying the mitochondrial genome of Sakha BC patients using the sequencing method.The purpose of the study was to identify BC-associated mitochondrial genome variants in Sakha patients.Material and Methods. The study included 14 Sakha patients diagnosed with BC. The median age of the patients was 49 years. DNA isolation was performed using phenol-chloroform extraction. DNA libraries were prepared using the Nextera Flex kit (Illumina, USA).Whole-genome sequencing of the mitochondrial genome was performed on a MiSeq instrument (Illuminа, USA). in the Shared Use Centre of the Research Institute of Medical Genetics, Tomsk National Research Centre of the Russian Academy of Sciences. The results obtained in BC patients were compared with those of control subjects.Results. In Sakha women with BC, 159 mitochondrial genome variants that differed from the human mitochondrial DNA (mtDNA) reference sequence (rCRS) were identified. Likely pathogenic variants m.3736G>A of the MT-ND1 gene and m.7279T>C of the MT-CO1 gene were shown to be associated with BC. For the first time, variants predisposing to BC (m.10398A>G; m.14783T>C; m.15043G>A; m.15301G>A) were identified. A distinctive feature of the mitochondrial genome of populations with a low BC incidence is a high level of mtDNA variants with changes in the length of the polycytosine region in the D310 locus.Conclusion. For the first time, mtDNA variants with changes in the length of the polycytosine tract in the D310 locus and likely pathogenic variants m.3736G>A of the MT-ND1 gene and m.7279T>C of the MT-CO1 gene were identified in Sakha BC women. The data obtained indicate that further studies on the role of the identified mtDNA variants in the development of BC using a larger sample of Sakha patients are required.
UNTUKMU.COM : THE CROWDSOURCING APPLICATION TO LEVERAGING THE USED OF SECONDHAND GOODS
Authors: Arifin Yulyani , Permai Syarifah Diana , Pudjihastuti Herena , Dwiputra Kristian , Faisal Fiandra Rheza , Marcellino Marcellino 
Published: January 2024

Information technology can be used in profit or non-profit activities. The concept of crowdsourcing has been widely used in fundraising in the form of money, better known as crowdfunding. In general, most people has secondhand items that are still good to use by others. On the other hand, there are also many people who need those items but cannot afford to buy them. A Website application with the Crowdsourcing concept can be used to bring together donors with recipients of donations in the form of goods.This study conducted data collection to assess the needs of application through questionnaires and analyzed the data using descriptive statistical analysis and Chi Square independence tests, and then developed a website-based application untukmu.com. This study examined the relationship between age, type of work and intention to donate and the type of goods needed. The Chi Square independence test showed that there is no relationship between age and type of work to the intention to donate. The descriptive statistics analysis showed that the majority (82%) of the types of goods usually donated by the donors are clothing. The results of the analysis provides input for the development of the application Untukmu.com. In the last stage, the study evaluated the level of satisfaction of the users (i.e. the donors) based on the level of ease of using the application and the time needed to master the application. The analysis showed that there is no relationship between the ease of use of the application and the donors satisfaction, but there is relationship between time needed to master the application to the user satisfaction. Untukmu.com application has a good usability level since users have no difficulty in using this application and are satisfied with the features available in the application.
Nanobiohybrids using organic nanoparticles for applications in water and wastewater treatment
Authors: Priyadarshini Upasana , Neelancherry Remya 
Published: January 2024

Abstract
To deal with emergencies like water shortages and water pollution, scientists have worked on making materials and technologies that function well enough. The current technologies are not ‘100%’ efficient at providing potable water. Therefore, it is essential to come up with new and better ways to purify water. Nanotechnology offers inventive water treatment techniques. Nanomaterials are fabricated with useful properties for adsorption, catalysis, sensing, and optoelectronics applications, such as reactivity, high-aspect ratio, controlled pore volume as well as electrostatic, hydrophilic, and hydrophobic interactions. Anions, metal ions, microorganisms, and organic compounds can be eliminated using nanoparticles (NPs). Minimal NP concentrations are required for water treatment, making their application cost-effective. Biopolymeric materials have a number of advantages over their synthetic counterparts due to their easy biodegradability and low impact on the environment. Because of their minimum toxicity, improved adsorption capability, excellent biodegradability, significant photocatalytic potential and biocompatibility, nanobiohybrids have a significant role in the field of water and wastewater treatment. This chapter summarizes various production methods of diversified nanobiohybrids synthesized in recent years, with a particular focus on nanobiocomposites.
Sustainable wastewater treatment using magnetic nanohybrids
Authors: Patil Anirudh Gururaj , Alangadu Kothandan Vivekanandan , Hemavathi A. B. , Suri Shweta , Pande Ranjana , Kagale Sneha , Yadav Snehal , Patil Gouri 
Published: January 2024

Abstract
Water pollution by various toxic contaminants has become one of the most serious problems across the globe. Various technologies have been used since ages to treat water and waste water that include filtration (membrane and gravel) and chemical (precipitation, coagulation, flocculation, adsorption, ion exchange, and electrochemical) methods. Although it requires an expensive material, commercial adsorbents are widely used in the industries for waste water treatment. Adsorption is generally used because of its adaptability in design and operation, reversibility, and feasibility. The choice of right and environmentally friendly adsorbent material for especially heavy metals has become the need of the hour. The nanomaterial/composites have demonstrated remarkable capacity for elimination of heavy metals from water. Different magnetic nanohybrid adsorbents have different adsorbing efficiencies for heavy metals due to their intrinsic properties such as surface area, functional group, zeta potential, and pore structure. Magnetic nanohybrid materials not only possess the properties of nanomaterials but also have magnetic properties that enable them as excellent adsorbents or catalysts in water pollution remediation. Also, extrinsic factors such as pH, temperature, and contact time influence the adsorption. In the last few decades, nanotechnology has received special attention and different nanomaterials or composites have been developed for water purification. Among which nanohybrid materials, combined with two or more compositions together possess a great advantage with new properties, diverse functionalities, and unlimited possibilities. In this chapter, sustainable waste water treatment with focus on nanohybrids is discussed that include sources of pollutants, methods of magnetic nanohybrid preparation, and factors influencing adsorption followed by applications and challenges.
Nanobiohybrids: a promising approach for sensing diverse environmental water pollutants
Authors: Jebarani D. Angelene Hannah , Alex Sruthi Ann 
Published: January 2024

Abstract
Water resource management has become the need of the hour with continuous depletion of good quality water. Sensors are important tools for monitoring the environmental water quality and quantifying the presence of various harmful pollutants (pesticides, heavy metals, and micro-organisms). Biosensors designed with functional biological molecules as recognition elements use a transducer, which converts a biological response into electrochemical, optical, gravimetric, magnetic, or thermal signals that can be easily quantified. The advent of nanotechnology has revolutionized material design and properties of sensing platforms. Materials in the nanoscale dimension possess interesting characteristic features such as high surface-area-to-volume ratio, high conductivity, functionalizability, shock-bearing ability, color tunability, photo-reactivity, and magnetization. Nanomaterial-integrated biomolecules with enhanced functional properties are termed as nanobiohybrids. The amalgamation of the tuneable characteristics of nanomaterials with functional biological molecules by means of functionalization and/or immobilization creates sophisticated transducers for enhancing the analytical performance of sensors. The resultant nanobiohybrid sensor systems are robust, exhibit relatively short response time with improved specificity and have higher sensitivity enabling detection of even trace volumes of analyte molecules. Recent advancements in research and engineering also promote the possibilities of device miniaturization, portability, and incessant monitoring of water quality. This chapter intends to discuss the impact of various toxic pollutants in water, the importance of nanomaterials in design of novel nanobiohybrids, the different nanomaterials relevant for use in design of sensors for environmental analysis, and finally the different transduction mechanisms that enable the detection and quantification of toxins in water.
Recent Advances in Electrochemical Sulfonylation using Sodium Sulfinates as
Sulfonyl Radical Precursors
Authors: Liang Sen , Gu Jia-Xin , Zeng Cheng-Chu 
Published: January 2024

Abstract:
Sodium sulfinates have been widely utilized as sulfonyl radical precursors for
preparing a diverse array of value-added sulfur-containing compounds (sulfones, sulfonamides,
sulfonates, thiosulfonates, etc.) through S-C, S-N, S-O and S-S bonds formation reactions.
Organic electrosynthesis has become an attractive alternative to conventional
methods for redox reactions because it utilizes electric current instead of chemical redox
agents. As such, the electrochemical generation of sulfonyl radicals from sodium sulfinates
and their applications in organic electrosynthesis have attracted much attention. In this
review, the recent advances in the electrochemical sulfonylation of organic compounds
involving sodium sulfinates as sulfonyl radical precursors since 2015 were reviewed, along
with related reaction mechanisms.
Recent Advances in the Preparation of Protein/peptide Microspheres by
Solvent Evaporation Method
Authors: Sun Huayan , Luo Weiwei , Huang Xiaowu 
Published: January 2024

:
Protein/peptide drugs are extensively used to treat various chronic and serious diseases.
The short half-life in vivo of protein and peptide as therapeutics drug limit the realization of
complete effects. Encapsulating drugs in microspheres can slow the speed of drug release and
prolong the efficacy of drugs. The solvent evaporation method is widely used to prepare protein/
peptide microspheres because of its facile operation and minimal equipment requirements.
This method has several challenges in the lower encapsulation efficiency, fluctuant release profiles
and the stabilization of protein/peptides, which researchers believe may be solved by adjusting
the preparation parameter or formulation of microspheres. The article discusses the formulation
parameters that govern the preparation of protein/peptide-loaded microspheres by the
solvent evaporation method, which provides an overview of the current promising strategies for
solvent evaporation for protein/peptide microspheres. The article takes parameter evaluation as
the framework, facilitating subsequent researchers to quickly find possible solutions when encountering
problems.
Pharmacological Action and Research Progress of Taraxasterol
Authors: Zhang Yu , Shaari Ramizu Bin , Awang Nawi Mohamad Arif Bin , Hassan Akram Bin , Cui Caiyun 
Published: January 2024

:
Primarily sourced from Asteraceae family herbs such as the Dandelion, Taraxasterol
is a pentacyclic triterpenoid lauded for its extensive biological functionalities. Its therapeutic
potency is demonstrated in various disease models, encompassing enteritis, arthritis, acute hepatic
injury, and pneumonia. Scientific literature underscores its anti-inflammatory, antioxidant,
and antineoplastic attributes. The primary aim of this study is to thoroughly explore the diseasemodulating
mechanisms and effects of taraxasterol. We endeavor to provide an exhaustive review
of the experimental subjects, intervention components, distinct action modalities, contributing
factors, and protein pathway expressions associated with taraxasterol, systematically represented
via diagrams and tables. Such a schematic representation encourages a continued academic
dialogue concerning taraxasterol's pharmacological characteristics. This review is envisioned
as a practical guide for the selection of experimental subjects and methodologies in prospective
research. It is intended to further illuminate taraxasterol's pharmacodynamics, thereby
offering theoretical and empirical justification for its clinical application.
MicroRNAs in Anticancer Drugs Hepatotoxicity: From Pathogenic
Mechanism and Early Diagnosis to Therapeutic Targeting by Natural
Products
Authors: Atteia Hebatallah Husseini 
Published: January 2024

:
Patients receiving cancer therapies experience severe adverse effects, including hepatotoxicity,
even at therapeutic doses. Consequently, monitoring patients on cancer therapy for
hepatic functioning is necessary to avoid permanent liver damage. Several pathways of anticancer
drug-induced hepatotoxicity involve microRNAs (miRNAs) via targeting mRNAs. These
short and non-coding RNAs undergo rapid modulation in non-targeted organs due to cancer
therapy insults. Recently, there has been an interest for miRNAs as useful and promising biomarkers
for monitoring toxicity since they have conserved sequences across species and are
cellular-specific, stable, released during injury, and simple to analyze. Herein, we tried to review
the literature handling miRNAs as mediators and biomarkers of anticancer drug-induced hepatotoxicity.
Natural products and phytochemicals are suggested as safe and effective candidates in
treating cancer. There is also an attempt to combine anticancer drugs with natural compounds to
enhance their efficiencies and reduce systemic toxicities. We also discussed natural products
protecting against chemotherapy hepatotoxicity via modulating miRNAs, given that miRNAs
have pathogenic and diagnostic roles in chemotherapy-induced hepatotoxicity and that many
natural products can potentially regulate their expression. Future studies should integrate these
findings into clinical trials by formulating suitable therapeutic dosages of natural products to
target miRNAs involved in anticancer drug hepatotoxicity.
Knowledge and Awareness of Emerging Cancer Therapies and their
Regulations among Budding Scientists in India: A Survey
Authors: Sharma Pinky , Jhawat Vikas , Singh Jatinder , Dutt Rohit 
Published: January 2024

Background:
Academic clinical research is considered the most important for cancer research
because it frequently tests novel drug combinations, investigates rarer diseases, and lowers the
risk for future commercial investments. However, due to the potential risks to the cancer patient, clinical
research is governed by strict regulations. In high-income countries, comprehensive cancer centers
(CCCs) have been established to align academic clinical cancer research with the regulatory
framework. In comparison, academic clinical cancer research is considered ineffective in low-income
countries.

Methods:
A cross-sectional, online survey was conducted to evaluate the knowledge of Indian health
science students regarding cutting-edge cancer therapeutics and their underlying regulatory requirements.

Results:
The survey found that 163 out of the 265 respondents were aware of the challenges of developing
safe and effective anticancer therapeutics. 43 respondents found no challenges, while 59
respondents were unaware of any. Out of 163, 44 respondents identified technical challenges, 31
identified regulatory issues, and 88 identified both challenges in developing novel anticancer therapeutics.
Interestingly, only 83 students out of 265, study cancer therapy regulations in their curriculum.
This clearly indicates that most of India's health science students have a significant lack of understanding
about the regulations for new cancer treatments.

Conclusion:
Academic clinical cancer research in India is just recognized as a prerequisite for degree
completion due to a lack of regulatory foundation. An emphasis should be placed on restructuring the
coursework offered to health science students to improve their ability to translate theoretical cancer
research to real-world clinical care.
Network Pharmacology and Bioinformatics Analyses Identify the Core Genes and Pyroptosis-Related Mechanisms of Nardostachys Chinensis for Atrial Fibrillation
Authors: Xue Weiqi , Luo Yuan , He Weifeng , Yan Mengyuan , Zhao Huanyi , Qing Lijin 
Published: January 2024

Background:
Nardostachys chinensis is an herbal medicine widely used in the treatment
of atrial fibrillation (AF), but the mechanism is unclear.

Objective:
To explore the molecular mechanism of N. chinensis against AF.

Methods:
The TCMSP was used to screen the active N. chinensis compounds and their targets.
Differentially expressed genes (DEGs) for AF were identified using open-access databases. Using
Venn diagrams, the cross-targets of N. chinensis, pyroptosis, and AF were obtained. The
genes underwent molecular docking as well as gene set enrichment analysis (GSEA). A nomogram
based on candidate genes was constructed and evaluated with the clinical impact curve.
After that, the immune infiltration of the dataset was analyzed by single sample GSEA
(ssGSEA). Finally, microRNAs (miRNAs) and transcription factors (TFs) were predicted based
on candidate genes.

Results:
Tumor necrosis factor (TNF) and caspase-8 (CASP8) were obtained as candidate genes
by taking the intersection of DEGs, targets of N. chinensis, and pyroptosis-related genes. Tolllike
receptor (TLR) and peroxisome proliferator-activated receptor (PPAR) signaling pathways
were linked to candidate genes. Additionally, immune cell infiltration analysis revealed that
CASP8 was associated with natural killer T cells, natural killer cells, regulatory T cells (Tregs),
myeloid-derived suppressor cells (MDSC), macrophages, CD8 T cells, and CD4 T cells. Finally,
miR-34a-5p and several TFs were found to regulate the expression of CASP8 and TNF.

Conclusion:
CASP8 and TNF are potential targets of N. chinensis intervention in pyroptosisrelated
AF, and the TLR/NLRP3 signaling pathway may be associated with this process.
Electrochemical Sensors Go Nano: Carbon Nanomaterials for Ultrasensitive Heavy Metal Analysis
Authors: Zhou Qingwei , Fu Li , Zhu Jiangwei 
Published: January 2024

Background:
Heavy metal contamination of food and the environment is a major
concern worldwide. Conventional detection techniques like atomic absorption spectroscopy
(AAS), inductively coupled plasma-optical emission spectrometry (ICP-OES) and inductively
coupled plasma-mass spectrometry (ICP-MS) have limitations including high costs and insufficient
sensitivity. Electrochemical sensors based on carbon nanomaterials have emerged as an
attractive alternative for rapid, affordable, and ultrasensitive heavy metal analysis.

Methods:
This review summarizes recent advances in using carbon nanomaterials like ordered
mesoporous carbon, carbon nanotubes, graphene and carbon dots for electrochemical sensing of
toxic heavy metals. Synthesis methods, characterization techniques, functionalization strategies
and detection mechanisms are discussed.

Results:
High surface area, electrical conductivity and electrocatalytic activity of carbon nanomaterials
enable preconcentration of metal ions and signal amplification at electrode interfaces.
This results in remarkably low detection limits down to parts per trillion levels. Functionalization
with metal nanoparticles, molecularly imprinted polymers and other nanocomposites further
improves sensor selectivity and sensitivity. These sensors have been applied for the quantitative
detection of arsenic, mercury, lead, cadmium, chromium, and other toxic metals in lab samples.

Conclusion:
Electrochemical sensors based on carbon nanotubes, graphene, mesoporous carbon,
and carbon dots are rapidly emerging as an ultrasensitive, cost-effective alternative to conventional
techniques for on-site screening of heavy metal contamination in food and environment.
Further validation using real-world samples and integration into portable field testing kits can
enable widespread deployment.
Identification of Mitophagy-Related Genes in Sepsis
Authors: Zeng Xiao-Yan , Zhang Min , Liao Si-Jing , Wang Yong , Ren Ying-Bo , Li Run , Li Tian-Mei , Mao An-Qiong , Li Guang-Zhen , Zhang Ying 
Published: January 2024

Background:
Numerous studies have shown that mitochondrial damage induces inflammation
and activates inflammatory cells, leading to sepsis, while sepsis, a systemic inflammatory
response syndrome, also exacerbates mitochondrial damage and hyperactivation. Mitochondrial
autophagy eliminates aged, abnormal or damaged mitochondria to reduce intracellular mitochondrial
stress and the release of mitochondria-associated molecules, thereby reducing the inflammatory
response and cellular damage caused by sepsis. In addition, mitochondrial autophagy
may also influence the onset and progression of sepsis, but the exact mechanisms are unclear.

Methods:
In this study, we mined the available publicly available microarray data in the GEO database
(Home - GEO - NCBI (nih.gov)) with the aim of identifying key genes associated with mitochondrial
autophagy in sepsis.

Results:
We identified four mitophagy-related genes in sepsis, TOMM20, TOMM22, TOMM40,
and MFN1.

Conclusion:
This study provides preliminary evidence for the treatment of sepsis and may provide
a solid foundation for subsequent biological studies.