bluuebunny/crossref_metadata_2025_split huggingface dataset:
48-hour Induction of Transdermal Buprenorphine to Extended-release Buprenorphine
Authors: Azar Pouya , Wong James S.H. , Mathew Nickie , Ignaszewski Martha J. , Partovi Nilufar , Krausz Reinhard M. , Ajidahun Adedayo , Thotakura Sahithi , Harris Marianne , Barrios Rolando , Montaner Julio S.G. , Maharaj Anil R. 
Published: October 2023

AbstractBuprenorphine extended-release (BUP-XR) provides sustained delivery of buprenorphine to control withdrawal and craving symptoms in the form of a monthly injectable and has been shown to improve health outcomes in patients with opioid use disorder. It is recommended that patients are stabilized with a transmucosal buprenorphine product, for at least 7 days per the product monograph; however, clinically, this timeline may be expedited. We report a case of a hospitalized patient with unregulated fentanyl use who underwent a successful transdermal buprenorphine induction for 48 hours to initiate BUP-XR with minimal levels of withdrawal and without precipitating opioid withdrawal. The approach described could provide a practical, patient-centered, accelerated induction strategy that, once independently validated, could considerably facilitate the use of BUP-XR.
Evidence-based management of optic neuritis
Authors: Bergeron Emilie , Bouffard Marc A. 
Published: October 2023

Purpose of review
Optic neuritis can result from several distinct causes, including multiple sclerosis (MS), neuromyelitis optica spectrum disorder (NMOSD), and myelin oligodendrocyte glycoprotein antibody disease (MOGAD), when not idiopathic. This review discusses evidence-based treatment approaches contingent upon each specific cause of optic neuritis.

Recent findings
Current evidence highlights the need for prompt plasmapheresis as adjunct to intravenous methylprednisolone (IVMP) in patients with NMOSD-associated optic neuritis. Recent advances have included a proliferation of novel disease modifying therapies (DMTs) for long-term management of NMOSD and an understanding of how existing therapeutic options can be leveraged to optimally treat MOGAD.

Summary
In acute idiopathic or MS-associated optic neuritis, IVMP hastens visual recovery, though it does not substantially affect final visual outcomes. IVMP and adjunctive plasmapheresis are beneficial in the treatment of NMOSD-associated optic neuritis, with a shorter time-to-treatment associated with a higher likelihood of recovery. The natural history of untreated MOGAD-associated optic neuritis is unclear but treatment with IVMP is near-universal given phenotypic similarities with NMOSD. Long-term immunosuppressive therapy is warranted in patients with NMOSD as well as in patients with MOGAD with poor visual recovery or recurrent attacks.
Epidermal cyst protruding from the occipital region of the scalp: a case report
Authors: Dahal Alok , Gupta Rakesh K. , Malla Justin J. , Koirala Diwakar , Baniya Aashish 
Published: October 2023

Introduction and importance:
Epidermal cysts are the most common subcutaneous tumor typically observed on the scalp, face, neck, back, or trunk. Epidermal cysts conventionally range in size from 1 to 5 cm, with sizes greater than 5 cm rarely reported.

Case presentation:
Here, we present a case of a 58-year-old female housewife who presented to our surgery outpatient department (OPD) with a history of a mass in the left occipital region. The mass was first noticed 3 years back and was small and associated with mild itching. The mass progressively increased in size over the course of 3 years. Her present complaint was a painful mass associated with itching, headache, and pus discharge when compressed. The pain was relieved by taking medication.

Clinical discussion:
The epidermal cyst was confirmed on biopsy, and subsequent excision of the cyst was done under general anesthesia; the occipital bone was eroded by the inferior part of the cyst – reconstruction of scalp defect done by rotational scalp flap.

Conclusion:
Epidermal cysts, being a slow-growing benign tumor, can pose diagnostic difficulties, especially when located in the scalp area. In addition to that, when cranial bones and intracranial structures are affected by the cyst, they can even lead to complications and interventional difficulties.
An inflammatory myofibroblastic tumor of the ampulla of Vater, an exceptional location: a case report
Authors: Zabeirou Aliou , Efared Boubacar , Souiki Tarek , James Didier Lassey , Majdoub Karim I. , Toughrai Imane , Chbani Laila , Mazaz Khalid 
Published: October 2023

Introduction and importance:
Inflammatory myofibroblastic tumors constitute a group of mesenchymal tumors associated with inflammatory infiltration. They occur mainly in young patients. It is classified by the World Health Organization as a borderline neoplasm. They are observed in many organs, particularly the lungs. Digestive localization is rare, and localization into the ampulla of Vater has been reported once.

Case presentation:
We report the case of a 39-year-old patient who was admitted for cholestatic jaundice with right hypochondrium pain. Computed tomography and magnetic resonance imaging revealed a tumor at the biliopancreatic junction. A cephalic duodenopancreatectomy was performed, and a histological examination of the surgical specimen revealed an inflammatory myofibroblastic tumor of the ampulla of Vater. The postoperative evolution was without any complications.

Clinical discussion:
This is the second case of localization of an inflammatory myofibroblastic tumor in Vater’s ampulla. The therapeutic approach is the complete excision of these inflammatory tumors, thus reducing the risk of local recurrence. In the literature, all cases of incomplete excision have resulted in recurrences.

Conclusion:
Inflammatory myofibroblastic tumors are rare. The diagnosis was based on histopathological findings and confirmed using immunohistochemical techniques.
Proximal radioulnar synostosis following Monteggia fracture-dislocation: a case report
Authors: Khadka Manoj , Pant Sakar , KC Manoj , Koirala Asmita , Bhandari Ravi , Poudel Arjun 
Published: October 2023

Introduction and importance:
Radioulnar synostosis is a rare complication of a forearm fracture that restricts pronation-supination. This study presents a case of proximal radioulnar synostosis in an adult male after Monteggia fracture-dislocation who had a loss of pronation and supination movements.

Case presentation:
Herein, we report a case of proximal radioulnar synostosis in a 43-year-old man who presented with loss of pronation and supination of the right forearm that restricted his daily activities. He had a history of Monteggia fracture-dislocation 9 months back, which was managed with open reduction and internal fixation with a dynamic compression plate. Plain radiography and computed tomography of the right forearm after 9 months of operation showed an implant in situ with proximal radioulnar synostosis. Implant removal was performed and the excess fibro-osseous connection in the proximal radius and ulna was removed.

Clinical discussion:
Forearm injuries that affect the interosseous membrane may result in radioulnar synostosis. Trauma and treatment-related factors increase the risk of radioulnar synostosis. The fibro-osseous fusion between the forearm bones restricts the pronation and supination movements.

Conclusion:
Loss of pronation-supination following forearm fracture should raise suspicion of radioulnar synostosis.
Pragmatic design and inclusion of patient–partner representatives improves participant experience in clinical research
Authors: Pogorzelski David , Wells Jeffrey L. , Marvel Debra , Palmer Jana E. , Mullins C. Daniel , Medeiros Michelle , Gallant Jodi L. , Spicer Ella , Bergin Patrick F. , Gitajn I. Leah , Mullin Devin S. , Gaski Greg E. , Hymes Robert , Bzovsky Sofia , Slobogean Gerard P. , Sprague Sheila ,   and the PREP-IT Investigators
Published: October 2023

Abstract

Objectives:
Patient engagement in the design and implementation of clinical trials is necessary to ensure that the research is relevant and responsive to patients. The PREP-IT trials, which include 2 pragmatic trials that evaluate different surgical preparation solutions in orthopaedic trauma patients, followed the patient-centered outcomes research (PCOR) methodology throughout the design, implementation, and conduct. We conducted a substudy within the PREP-IT trials to explore participants' experiences with trial participation.

Methods:
At the final follow-up visit (12 months after their fracture), patients participating in the PREP-IT trials were invited to participate in the substudy. After providing informed consent, participants completed a questionnaire that asked about their experience and satisfaction with participating in the PREP-IT trials. Descriptive statistics are used to report the findings.

Results:
Four hundred two participants participated in the substudy. Most participants (394 [98%]) reported a positive experience, and 376 (94%) participants felt their contributions were appreciated. The primary reasons for participation were helping future patients with fracture (279 [69%]) and to contribute to science (223 [56%]). Two hundred seventeen (46%) participants indicated that their decision to participate was influenced by the minimal time commitment.

Conclusions:
Most participants reported a positive experience with participating in the PREP-IT trials. Altruism was the largest motivator for participating in this research. Approximately half of the participants indicated that the pragmatic, low-participant burden design of the trial influenced their decision to participate. Meaningful patient engagement, a pragmatic, and low-burden protocol led to high levels of participant satisfaction.
An Assessment of Presentation Slide Quality at a National Hand Surgery Meeting
Authors: Nasser Jacob S. , Wood Shannon M. , Horiuchi Sakura , Chung Kevin C. 
Published: October 2023

Background:
Effective information transfer relies on the proper use of educational tools. Evaluating the quality of presentations permits us to improve educational materials in plastic surgery. The authors’ aims were to assess the quality of presentations at a national hand surgery meeting, using a checklist of presentation standards from the literature, and to identify areas of improvement.

Methods:
The study sample included presentations from the clinical papers sessions at the 2020 Annual Meeting of the American Society for Surgery of the Hand. A modified checklist based on the literature was used to assess the presentations. Two members of the research team extracted data from the included presentations, and disagreements were reviewed collaboratively.

Results:
A total of 96 presentations were included in this sample. The mean number of deficiencies per slide set was approximately 9. Misused graphics, ambiguous content (eg, undefined abbreviations, undefined symbols), and overdetermined slides were the most common deficiencies identified in the sample. One-way analysis of variance of presenter role found a significant difference in the mean number of deficiencies (F
2,93 = 7.36; P = 0.001) among different types of presenters, with surgeon presenters exhibiting more deficiencies than students and other health care professionals.

Conclusions:
The use of a checklist to evaluate a presentation helps cultivate more effective presentations in national meetings. A collaborative peer-review process, incorporating feedback from multiple trainees, audience members, and colleagues, facilitates effective information transfer through presentations.
Treatment approaches for severe Stenotrophomonas maltophilia infections
Authors: Mojica Maria F. , Bonomo Robert A. , van Duin David 
Published: October 2023

Purpose of review

Stenotrophomonas maltophilia is an emerged opportunistic pathogen. Intrinsic multidrug resistance makes treating infections caused by S. maltophilia a great clinical challenge. Herein, we provide an update on the most recent literature on treatment options for severe S. maltophilia infections.

Recent findings
Trimethoprim-sulfamethoxazole (SXT) is recognized as the first-line therapy for S. maltophilia infections. However, its clinical use is based on good in vitro activity and favorable clinical outcomes, rather than on solid minimum inhibitory concentration (MIC) correlations with pharmacokinetic/pharmacodynamics (PK/PD) and/or clinical outcomes. The same is true for other treatment options like levofloxacin (LVX) and minocycline (MIN). Recent PK/PD studies question the current clinical breakpoints for SXT, LVX, and MIN. Based on this, the latest guidance issued by the Infectious Diseases Society of America (IDSA) recommends using these agents only as part of a combination therapy. Alternatively, novel therapeutic options such as cefiderocol (FDC) and ceftazidime-avibactam plus aztreonam (CZA-ATM) are suggested, based on limited but promising clinical data.

Summary
PK/PD data and controlled clinical studies are needed to optimize current treatment options. Presently, combination therapy of SXT, LVX, MIN, or FDC, or monotherapy with CZA-ATM are recommended therapeutic options for severe-to-moderate S. maltophilia infections.
Axillary Lymph Node Metastasis as the First Symptom of Colon Cancer
Authors: Zhao Weiwei , Li Peng , Li Yanmei , He Yueming , Yang Jianwei 
Published: October 2023

Abstract
Axillary lymph node metastasis in colon cancer is extremely rare. We describe the FDG PET/CT findings in a 61-year-old woman who presented with a mass in the right axilla, which revealed irregular thickening of the ascending colon wall, as well as multiple enlarged lymph nodes in the ileocecal mesentery, para-aortic region, and right axilla with higher uptake of FDG. Ascending colon carcinoma with lymph node metastasis in the right axilla was confirmed by pathological examination.
Rapid and high yield isolation of extracellular vesicles with purity by application of size exclusion fast performance liquid chromatography
Authors: Kapoor Kshipra S. , Harris Kristen , Arian Kent , Ma Lihua , Church Kaira A. , Kalluri Raghu 
Published: October 2023

AbstractExtracellular Vesicles (EVs) have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures. Extracellular vesicles also offer an advantage compared to synthetic vesicles, for delivery of various drugs. However, limitations in segregating EVs from soluble proteins have led to inconsistent EV retrieval rates with low levels of purity. Here, we report a new high-yield (>95%) and rapid (<20 min) EV isolation method calledSizeExclusion –FastPerformanceLiquidChromatography (SE-FPLC). We show SE-FPLC can effectively isolate EVs from multiple sources including EVs derived from human and mouse cells and serum. The results indicate that SE-FPLC can successfully remove highly abundant protein contaminants such as albumin and lipoprotein complexes, which can represent a major hurdle in large scale isolation of EVs for clinical translation. Additionally, the high-yield nature of SE- FPLC allows for easy industrial upscaling of extracellular vesicles production for various clinical utilities. Moreover, SE-FPLC enables analysis of very small volumes of blood for use in point-of-care diagnostics in the clinic. Collectively, SE-FPLC offers many advantages over current EV isolation methods and offers rapid clinical utility potential.
Maternal exposure to prostaglandin e2 results in abnormal dendritic morphology in the cerebellum and related motor behaviour in mouse offspring
Authors: Kissoondoyal Ashby , Ho Kelly , Wong Christine , Crawford Dorota A. 
Published: October 2023

AbstractThe lipid signalling molecule prostaglandin E2 (PGE2) is important in healthy brain development. Abnormal PGE2 levels during prenatal development, which can be influenced by genetic causes and exposure to various environmental risk factors, have been linked to increased prevalence of Autism Spectrum Disorders (ASDs). Growing research in animal models aims to provide evidence for the mechanisms by which increased or reduced PGE2 levels influence brain development. In this study, we show that maternal exposure to PGE2 in mice at gestational day 11 (G11) results in molecular changes within the cerebellum and associated behaviours in offspring. We observed a decrease in cerebellar cell density originating at G11 (in males and females) and at G16 (in females only). In Golgi-COX-stained cerebellar slices from PGE2-exposed offspring at the postnatal day 30 (PN30), we found an increase in dendritic arborization, the odds of observing dendritic loops, dendritic spine density, and the odds of observing mature (mushroom-shaped) spines. We also observed a decrease in the expression level of the cytoskeletal protein β-actin, the actin associated protein spinophilin, and the cell adhesion protein N-Cadherin. In addition, we found that specifically PGE2-exposed male offspring exhibited abnormal cerebellar related motor function. This study adds further evidence that changes in the PGE2 levels during critical times may impact the developing brain differently in males and females. These findings also emphasize the importance of examining sex differences in research relevant to neurodevelopmental disorders.
Analysis the molecular similarity of least common amino acid sites in ACE2 receptor to predict the potential susceptible species for SARS-CoV-2
Authors: Hu YeZhi , Fan Xin , Villalan Arivizhivendhan Kannan , Zhang Shuang , Joka Fekede Regassa , Wu XiaoDong , Wang HaoNing , Wang XiaoLong 
Published: October 2023

AbstractThis research offers a bioinformatics approach to forecasting both domestic and wild animals’ likelihood of being susceptible to SARS-CoV-2 infection. Genomic sequencing can resolve phylogenetic relationships between the virus and the susceptible host. The genome sequence of SARS-CoV-2 is highly interactive with the specific sequence region of the ACE2 receptor of the host species. We further evaluate this concept to identify the most important SARS-CoV-2 binding amino acid sites in the ACE2 receptor sequence through the common similarity of the last common amino acid sites (LCAS) in known susceptible host species. Therefore, the SARS-CoV-2 viral genomic interacting key amino acid region in the ACE2 receptor sequence of known susceptible human host was summarized and compared with other reported known SARS-CoV-2 susceptible host species. We identified the 10 most significant amino acid sites for interaction with SARS-CoV-2 infection from the ACE2 receptor sequence region based on the LCAS similarity pattern in known sensitive SARS-CoV-2 hosts. The most significant 10 LCAS were further compared with ACE2 receptor sequences of unknown species to evaluate the similarity of the last common amino acid pattern (LCAP). We predicted the probability of SARS-CoV-2 infection risk in unknown species through the LCAS similarity pattern. This method can be used as a screening tool to assess the risk of SARS-CoV-2 infection in domestic and wild animals to prevent outbreaks of infection.Graphical abstract
Antigen-level resolution of commensal-specific B cell responses enabled by phage-display screening and B cell tetramers
Authors: Verma Sheenam , Dufort Matthew J. , Olsen Tayla M. , Labuda Jasmine C. , Kimmel Samantha , Scharffenberger Sam , McGuire Andrew T. , Harrison Oliver J. 
Published: October 2023

SummaryInduction of adaptive immune responses to commensal microbes is critical for tissue homeostasis, and perturbation of these responses is associated with multiple chronic inflammatory disorders. However, the mechanisms underlying the induction and regulation of mucosal B cells targeting commensal microbes remain poorly understood, in part due to a lack of tools to identify commensal-specific B cellsex vivo. To address this, we identified immunogenic protein epitopes recognized by Segmented Filamentous Bacteria (SFB)-specific serum antibodies using a whole-genome phage display screen and identified immunogenic proteins engaging IgA, IgG1and IgG2bresponses. Using these antigens, we generated B cell tetramers to identify and track SFB-specific B cell responses in the gut associated lymphoid tissue during natural andde novocolonization. We revealed a compartmentalized response in SFB-specific B cell activation between Peyer’s patches and mesenteric lymph nodes, with a gradient of IgA, IgG1and IgG2bisotypes along the small intestine, and selective production of IgG2bwith the mesenteric lymph node chain. VDJ sequencing analyses and generation of SFB-specific monoclonal antibodies identified that somatic hypermutation drives affinity maturation to SFB derived antigens under homeostatic conditions. By combining phage display screening and B cell tetramer technologies, we now enable antigen-level based studies of immunity to intestinal microbes, which will advance our understanding of the ontogeny and function of commensal-specific B cell responses in tissue immunity, inflammation and repair.
MET receptor activation by stromal cells serves as promising target in melanoma brain metastases
Authors: Redmer Torben , Schumann Elisa , Peters Kristin , Weidemeier Martin E. , Nowak Stephan , Schroeder Henry W.S. , Vidal Anna , Radbruch Helena , Lehmann Annika , Kreuzer-Redmer Susanne , Jürchott Karsten , Radke Josefine 
Published: October 2023

AbstractThe development of brain metastases hallmarks disease progression in 20-40% of melanoma patients and is a serious obstacle to therapy. Understanding the processes involved in the development and maintenance of melanoma brain metastases (MBM) is critical for the discovery of novel therapeutic strategies. Here, we generated transcriptome and methylome profiles of MBM showing high or low abundance of infiltrated Iba1hightumor-associated microglia and macrophages (TAMs). Our survey identified potential prognostic markers of favorable disease course and response to immune checkpoint inhibitor (ICi) therapy, among themAPBB1IPand the interferon-responsive geneITGB7. In MBM with high ITGB7/APBB1IP levels, the accumulation of TAMs correlated significantly with the immune score. Signature-based deconvolution of MBM via single sample GSEA revealed enrichment of interferon-response and immune signatures and revealed inflammation, stress and MET receptor signaling. MET receptor phosphorylation/activation maybe elicited by inflammatory processes in brain metastatic melanoma cells via stroma cell-released HGF. We observed phospho-METY1234/1235in a subset of MBM and observed marked response of brain metastasis-derived cell lines (BMCs) that lacked druggable BRAF mutations or developed resistance to BRAF inhibitors (BRAFi)in vivoto MET inhibitors PHA-665752 and ARQ197 (tivantinib). In summary, the activation of MET receptor in brain colonizing melanoma cells by stromal cell-released HGF may promote tumor cells self-maintenance and expansion might counteract ICi therapy. Therefore, therapeutic targeting of MET possibly serves as promising strategy to control intracranial progressive disease and improve patient survival.