bluuebunny/crossref_metadata_2025_split huggingface dataset:
A 3D urban scene reconstruction enhancement approach based on adaptive viewpoint selection of panoramic videos
Authors: Zhang Xujie , Hu Zhenbiao , Hu Qingwu , Zhao Jun , Ai Mingyao , Zhao Pengcheng , Li Jiayuan , Zhou Xiaojie , Chen Zongqiang 
Published: January 2024

AbstractThe widely used unmanned aerial vehicle oblique photogrammetry often suffers from information loss in complex urban environments, leading to geometric and textural defects in the resulting models. In this study, a close‐range panoramic optimal viewpoint selection assisted 3D urban scene reconstruction enhancement method is proposed for areas prone to defects. We first introduce the ground panoramic data acquisition equipment and strategy, which are different from those of the single‐lens supplementary photography method. Data acquisition is accomplished through a single and continuous surround‐style collection approach. The full coverage of the panoramic video in the space–time dimension enables the acquisition of texture details without considering camera station planning. Then, a panoramic multiview image generation approach is proposed. Adaptive viewpoint selection is achieved using unbiased sampling points from the rough scene model, and viewpoint optimisation is adopted to ensure sufficient image overlap and intersection effects, thus improving the scene reconstructability. Finally, the 3D model is generated by photogrammetric processing of the panoramic multiview images, resulting in an enhanced modelling effect. To validate the proposed method, we conducted experiments using real data from Qingdao, China. Both the qualitative and quantitative results demonstrate a significant improvement in the quality of geometric and textural reconstruction. The tie‐point reprojection errors are less than 1 pixel, and the registration accuracy with the model from oblique photogrammetry is comparable to that of optimised‐view photography. By eliminating the need for on‐site camera station planning or manual flight operations and effectively minimising the redundancy of panoramic videos, our approach significantly reduces the photography and computation costs associated with reconstruction enhancement. Thus, it presents a feasible technical solution for the generation of urban 3D fine models.
Enzymatically modified isoquercitrin and its protective effects against photoaging: In‐vitro and clinical studies
Authors: Bai Xue‐dong , Fei Wei‐cheng , Liu Yu‐chen , Yang Sheng‐ping 
Published: January 2024

AbstractThis research examines the anti‐aging potential of the flavonoid derivative of isoquercitrin known as enzymatically modified isoquercitrin (EMIQ). Initial HPLC analyses showed that EMIQ used in the study contained 1–12 glucosides and 10.7% pentahydroxyflavonoids, promising potent antioxidant properties. In subsequent in‐vitro studies with UVA‐exposed human dermal fibroblasts (HDFa), EMIQ demonstrated protective properties by reducing collagen damage. It modulated both the TGFβ/Smad pathway and the MMP1 pathway, contributing to collagen preservation. This protective effect was further confirmed using the T‐Skin™ model, a reconstructed full‐thickness human skin model, which illustrated that EMIQ could defend the physiological structures of both the epidermis and dermis against UV radiation. A 28‐day clinical trial with 30 volunteers aged 31–55 years highlighted EMIQ's effectiveness. Participants using EMIQ‐containing Essence displayed reduced facial trans‐epidermal water loss and skin roughness, alongside improved skin elasticity. This study emphasizes EMIQ's potential as an anti‐photoaging ingredient in cosmetics, warranting further research. The findings pave the way for developing innovative skincare products addressing photoaging effects.
InsurTech in the United States and Germany—What are the drivers behind the different business models?
Authors: Oletzky Torsten 
Published: January 2024

AbstractComparing the InsurTech ecosystems of the United States and Germany (Europe), there are significant regional differences in the choice of business models. While many InsurTechs in the United States have opted for the business model of a fully licensed insurer, this business model is much less common in Europe. In Europe, many InsurTechs seem to shy away from applying for a license as an insurer and limit themselves to the business model of a broker or a managing general agent. This paper analyzes the factors that influence an InsurTech's choice of business model when deciding whether or not to apply for an insurance license. It examines the impact of different local market environments on these decisions, as well as the role that access to venture capital plays in business model decisions and how regulators and their actions influence the decision‐making process.
The expression and significance of PD‐L1 in condyloma acuminatum
Authors: Liu Yamei , Huang Weiqi , Yang Kun , Du Xiangxi , Guo Xueyun , Cao Yuchun 
Published: January 2024

AbstractBackgroundIt has been reported that programmed death‐ligand 1 (PD‐L1) is highly expressed in cells during viral infection, which helps the virus escape host immunity. However, the relationship between human papillomavirus (HPV) and PD‐L1 in condyloma acuminatum and whether they participate in immunosuppression have not been reported. In this paper, we aimed to explore the expression and significance of PD‐L1 in condyloma acuminatum.MethodsThe expression of PD‐L1 in the wart of condyloma acuminatum patients and the foreskin of healthy individuals was evaluated. Lentivirus transfection was used to introduce the HPV11‐E7 gene into HaCaT cells to investigate whether HPV infection could affect the expression of PD‐L1. The successfully constructed HPV11‐E7 HaCaT cells were cocultured with Jurkat cells, and Jurkat cell apoptosis and proliferation as well as the Jurkat cell cycle were evaluated by flow cytometry and cell counting kit‐8 (CCK‐8) assays.ResultsPD‐L1 was highly expressed in keratinocytes of genital warts. Through the construction of a cell model, we found that HPV11‐E7 could upregulate the expression of PD‐L1 in HaCaT cells. Furthermore, HPV11‐E7 HaCaT cells can promote the apoptosis of Jurkat cells, inhibit the proliferation of Jurkat cells and mediate the cell cycle arrest of Jurkat cells through the PD‐1/PD‐L1 signalling pathway.ConclusionsHPV infection may upregulate PD‐L1 expression in the keratinocytes of genital warts and participate in the inhibition of local T‐cell function.
Phylogenomic reconstruction illuminates the evolutionary history of freshwater to marine transition in the subfamily Haloveliinae (Hemiptera: Heteroptera: Veliidae)
Authors: Ye Zhen , Jin Zezhong , Polhemus Dan A. , Wang Siqi , Fu Siying , Yang Huanhuan , Qiao Mu , Bu Wenjun 
Published: January 2024

AbstractWater crickets of the subfamily Haloveliinae are semi‐aquatic bugs occurring in freshwater and marine habitats throughout the Indo‐Australian region, presently including six genera with more than 80 extant species. Whether lineage diversification in Haloveliinae is associated with the utilization of new ecological niches caused by transition events between freshwater and marine habitats remains poorly understood. We investigate the evolutionary history of Haloveliinae using large‐scale phylogenomic datasets and a set of novel redefined morphological characters based on 24 ingroup taxa representing all recognized genera. Our phylogenetic results based on the novel datasets definitively indicate that the freshwater genus Strongylovelia Esaki as currently defined is paraphyletic and supports the establishment of a new genus: Metavelia gen. nov., including three congeneric species: Metavelia patiooni comb. nov. (type species), Metavelia priori comb. nov. and Metavelia albicolli comb. nov. Reconstruction of ancestral habitats suggests a freshwater origin for the Haloveliinae. Divergence time estimations reveal that the origin of the monophyletic marine clade occurred at around 83 Ma (95% highest posterior density: 71–98 Ma) in the Late Cretaceous, involving a single transition event from freshwater to marine habitats. This time coincides with the period of high global sea levels in the Late Cretaceous. During this period, the marine incursions caused by the massive sea level rise flooded the continental margins, especially in Southeast Asia, where ancestral Haloveliinae were probably distributed. The appearance of new marine habitats after the marine incursions (e.g., intertidal, mangroves and estuarine) probably led to a subsequent establishment and diversification of the marine lineages.
Physiological assessment of a 16 day, 4385 km ultra‐endurance mountain bike race: A case study
Authors: Hyldahl Robert D. , Gifford Jayson R. , Davidson Lance E. , Hancock Chad R. , Hafen Paul S. , Parcell Allen C. , Mack Gary W. 
Published: January 2024

AbstractThe Tour Divide (TD) is a 4385 km ultra‐endurance bicycle race that follows the continental divide from Canada to Mexico. In this case study, we performed a comprehensive molecular and physiological profile before and after the completion of the TD. Assessments were performed 35 days before the start (Pre‐TD) and ∼36 h after the finish (Post‐TD). Total energy expenditure was assessed during the first 9 days by doubly labelled water (2H218O), abdominal and leg tissue volumes via MRI, and graded exercise tests to quantify fitness and substrate preference. Vastus lateralis muscle biopsies were taken to measure mitochondrial function via respirometry, and vascular function was assessed using Doppler ultrasound. The 47‐year‐old male subject took 16 days 7 h 45 min to complete the route. He rode an average of 16.8 h/day. Neither maximal O2 uptake nor maximal power output changed pre‐ to post‐TD. Measurement of total energy expenditure and dietary recall records suggested maintenance of energy balance, which was supported by the lack of change in body weight. The subject lost both appendicular and trunk fat mass and gained leg lean mass pre‐ to post‐TD. Skeletal muscle mitochondrial and vascular endothelial function decreased pre‐ to post‐TD. Overall, exercise performance was maintained despite reductions in muscle mitochondrial and vascular endothelial function post‐TD, suggesting a metabolic reserve in our highly trained athlete.
Two rare variants that affect the same amino acid in CFTR have distinct responses to ivacaftor
Authors: Li Hongyu , Rodrat Mayuree , Al‐Salmani Majid K. , Veselu Diana‐Florentina , Han Sangwoo T. , Raraigh Karen S. , Cutting Garry R. , Sheppard David N. 
Published: January 2024

AbstractSome residues in the cystic fibrosis transmembrane conductance regulator (CFTR) channel are the site of more than one CFTR variant that cause cystic fibrosis. Here, we investigated the function of S1159F and S1159P, two variants associated with different clinical phenotypes, which affect the same pore‐lining residue in transmembrane segment 12 that are both strongly potentiated by ivacaftor when expressed in CFBE41o− bronchial epithelial cells. To study the single‐channel behaviour of CFTR, we applied the patch‐clamp technique to Chinese hamster ovary cells heterologously expressing CFTR variants incubated at 27°C to enhance channel residence at the plasma membrane. S1159F‐ and S1159P‐CFTR formed Cl− channels activated by cAMP‐dependent phosphorylation and gated by ATP that exhibited thermostability at 37°C. Both variants modestly reduced the single‐channel conductance of CFTR. By severely attenuating channel gating, S1159F‐ and S1159P‐CFTR reduced the open probability (Po) of wild‐type CFTR by ≥75% at ATP (1 mM); S1159F‐CFTR caused the greater decrease in Po consistent with its more severe clinical phenotype. Ivacaftor (10–100 nM) doubled the Po of both CFTR variants without restoring Po values to wild‐type levels, but concomitantly, ivacaftor decreased current flow through open channels. For S1159F‐CFTR, the reduction of current flow was marked at high (supersaturated) ivacaftor concentrations (0.5–1 μM) and voltage‐independent, identifying an additional detrimental action of elevated ivacaftor concentrations. In conclusion, S1159F and S1159P are gating variants, which also affect CFTR processing and conduction, but not stability, necessitating the use of combinations of CFTR modulators to optimally restore their channel activity.
imageKey points
Dysfunction of the ion channel cystic fibrosis transmembrane conductance regulator (CFTR) causes the genetic disease cystic fibrosis (CF).
This study investigated two rare pathogenic CFTR variants, S1159F and S1159P, which affect the same amino acid in CFTR, to understand the molecular basis of disease and response to the CFTR‐targeted therapy ivacaftor.
Both rare variants diminished CFTR function by modestly reducing current flow through the channel and severely inhibiting ATP‐dependent channel gating with S1159F exerting the stronger adverse effect, which correlates with its association with more severe disease.
Ivacaftor potentiated channel gating by both rare variants without restoring their activity to wild‐type levels, but concurrently reduced current flow through open channels, particularly those of S1159F‐CFTR.
Our data demonstrate that S1159F and S1159P cause CFTR dysfunction by multiple mechanisms that require combinations of CFTR‐targeted therapies to fully restore channel function.
Inhibition of
Pseudomonas aeruginosa
quorum sensing by chemical induction of the MexEF-oprN efflux pump
Authors: Kristensen Rasmus , Andersen Jens Bo , Rybtke Morten , Jansen Charlotte Uldahl , Fritz Blaine Gabriel , Kiilerich Rikke Overgaard , Uhd Jesper , Bjarnsholt Thomas , Qvortrup Katrine , Tolker-Nielsen Tim , Givskov Michael , Jakobsen Tim Holm 
Published: January 2024

ABSTRACT

The cell-to-cell communication system quorum sensing (QS), used by various pathogenic bacteria to synchronize gene expression and increase host invasion potentials, is studied as a potential target for persistent infection control. To search for novel molecules targeting the QS system in the Gram-negative opportunistic pathogen
Pseudomonas aeruginosa
, a chemical library consisting of 3,280 small compounds from LifeArc was screened. A series of 10 conjugated phenones that have not previously been reported to target bacteria were identified as inhibitors of QS in
P. aeruginosa
. Two lead compounds (ethylthio enynone and propylthio enynone) were re-synthesized for verification of activity and further elucidation of the mode of action. The isomeric pure Z-ethylthio enynone was used for RNA sequencing, revealing a strong inhibitor of QS-regulated genes, and the QS-regulated virulence factors rhamnolipid and pyocyanin were significantly decreased by treatment with the compounds. A transposon mutagenesis screen performed in a newly constructed
lasB-gfp
monitor strain identified the target of Z-ethylthio enynone in
P. aeruginosa
to be the MexEF-OprN efflux pump, which was further established using defined
mex
knockout mutants. Our data indicate that the QS inhibitory capabilities of Z-ethylthio enynone were caused by the drainage of intracellular signal molecules as a response to chemical-induced stimulation of the MexEF-oprN efflux pump, thereby inhibiting the autogenerated positive feedback and its enhanced signal-molecule synthesis.
Proinflammatory CD14
high
CD16
low
monocytes/macrophages prevail in
Treponema phagedenis-
associated bovine digital dermatitis
Authors: Lahiri Priyoshi , Arrazuria Rakel , Tan Yi Lin , De Buck Jeroen , Hollenberg Morley D. , Orsel Karin , Cobo Eduardo R. 
Published: January 2024

ABSTRACT

Digital dermatitis (DD) is a skin disease in cattle characterized by painful inflammatory ulcerative lesions in the feet, mostly associated with local colonization by
Treponema
spp., including
Treponema phagedenis
. The reason why most DD lesions remain actively inflamed and progress to chronic conditions despite antibiotic treatment remains unknown. Herein, we show an abundant infiltration of proinflammatory (CD14
high
CD16
low
) monocytes/macrophages in active DD lesions, a skin response that was not mitigated by topical treatment with oxytetracycline. The associated bacterium,
T. phagedenis
, isolated from DD lesions in cattle, when injected subcutaneously into mice, induced abscesses with a local recruitment of Ly6G
+
neutrophils and proinflammatory (Ly6C
high
CCR2
+
) monocytes/macrophages, which appeared at infection onset (4 days post challenge) and persisted for at least 7 days post challenge. When exploring the ability of macrophages to regulate inflammation, we showed that bovine blood-derived macrophages challenged with live
T. phagedenis
or its structural components secreted IL-1β via a mechanism dependent on the NLRP3 inflammasome. This study shows that proinflammatory characteristics of monocytes/macrophages and neutrophils dominate active non-healing ulcerative lesions in active DD, thus likely impeding wound healing after antibiotic treatment.
Pervasive Neoarchean melting of subducted sediments generating sanukitoid and associated magmatism in the North China Craton, with implications for the operation of plate tectonics
Authors: Li Jialiang , Liu Sheng-Ao , Ma Haibo , Wu Chen , Zhu Di-Cheng , Liu Jingao 
Published: January 2024

Abstract
The Mesoarchean to Neoarchean period (ca. 3.0–2.5 Ga) is the most important stage during the emergence and evolution of plate tectonics. However, plate subduction at this time may have been less stable and perhaps more susceptible to the lubrication effect of sediments than the modern counterpart. Such predictions have not yet been verified by field-based investigations. In this work, we identified two types of rock units (i.e., sanukitoids and associated adakitic suites, exposed in the Eastern Hebei Complex of the North China Craton) and illustrated their petrogenesis and tectonic context through field, geochronologic, geochemical, and isotopic investigations. Laser ablation–inductively coupled plasma–mass spectrometry zircon U-Pb analyses suggest that the two magmatic suites formed within a relatively short time span of ca. 2596–2544 Ma and ca. 2559–2533 Ma, respectively. The sanukitoids are composed of meta-andesites and diorite porphyrites and characterized by relatively high MgO (3.94–5.62 wt%), Mg# (50–61), Cr (73–343 ppm), and Ni (37–111 ppm) values. The adakitic rocks are composed of granodiorite-granite gneisses and have relatively high Sr (316–1001 ppm) and low Y (7–13 ppm) and Yb (0.83–1.37 ppm) contents, and relatively high Sr/Y (36–89) and La/Yb (16–45) ratios. Rocks from both suites exhibit depletions of Nb, Ta, and Ti and have similar Sr-Nd-Hf-Zn isotopes: variable (87Sr/86Sr)i (0.7002–0.7053), weakly positive εNd(t) (+0.3 to +1.7) and εHf(t) (+1.8 to +6.8), and slightly heavy δ66Zn (0.30‰–0.36‰). These geochemical characteristics indicate that the sanukitoids were derived from the melting of subducted sediments followed by melt-mantle interaction, whereas the adakitic rocks were produced by direct partial melting of subducted plate (including tonalite-trondjhemite-granodiorite melts) under a garnet stability field with minor sediments. Such distinct magmatic rock associations, together with the coeval charnockites and tholeiites with diverse compositions in the adjacent area, can be best explained by a slab breakoff model. Further, events associated with slab breakoff are likely to represent a transition of a quasi-plate tectonic regime, characterized by multiple, continuous, and stagnant attempts to start the modern-style subduction on Earth. In addition, the emergence of sanukitoids and associated magmas symbolized the onset of supracrustal recycling into the mantle. Combined with the Nd-Hf-Zn isotopes of diverse magmatic rocks in the North China Craton that are comparable to other Precambrian magmatic rock suites worldwide, we suggest that supracrustal recycling symbolized the onset of plate tectonics since ca. 3.0 Ga, and by inference played a key role in the development of subduction-driven plate tectonics in addition to Earth’s secular cooling.
A Bayesian astrochronology for the Cambrian first occurrence of trilobites in West Gondwana (Morocco)
Authors: Sinnesael Matthias , Millard Andrew R. , Smith Martin R. 
Published: January 2024

AbstractThe first occurrence of trilobites at ca. 520 Ma is an iconic feature of the Cambrian Explosion. Developing a robust evolutionary view on early Cambrian life is generally hindered by large uncertainties in the ages of fossil finds and their global stratigraphic correlation. We developed an astrochronological interpretation for the Tiout section in Morocco that features some of the oldest trilobite fossils. Our novel approach to incorporating individual astronomical cycle durations in an integrated radioisotopic and astrochronological Bayesian age-depth model results in an age estimate of 519.62 Ma (519.70–519.54 Ma 95% highest posterior distribution) for the first occurrence of trilobites in West Gondwana. This level of precise age estimation is exceptional for biological events in deep time and demonstrates the power of our novel approach.
Parameters of Cell Death and Proliferation of Prostate Cancer Cells with Altered Expression of Myosin 1C Isoforms
Authors: Solomatina E. S. , Nishkomaeva E. N. , Kovaleva A. V. , Tvorogova A. V. , Potashnikova D. M. , Saidova A. A. 
Published: January 2024

Abstract
Myosin 1C is a monomeric myosin motor with a truncated tail domain. Such motors are referred as slow “tension sensors.” Three isoforms of myosin 1C differ in short N-termed amino acid sequences, the functional differences between isoforms have not been elucidated. Myosin 1C isoform A was described as a diagnostic marker for prostate cancer, but its role in tumor transformation remains unknown. Based on data on the functions of myosin 1C, we hypothesized the potential role of myosin 1C isoforms in maintaining the tumor phenotype of prostate cancer cells. In our work, we showed that a decrease in the expression level of myosin 1C isoform C leads to an increase in the proliferative activity of prostate tumor cells.
HspBP1 in Complex with the Peptide of the Innate Immunity Protein Tag7 is Able to Lyse Tumor Cells Carrying TNFR1 Receptor
Authors: Romanova E. A. , Yurkina D. M. , Yashin D. V. , Sashchenko L. P. , Georgiev G. P. 
Published: January 2024

Abstract
The search for new cytotoxic agents capable of lysing tumor cells is an important task in the fight against cancer. Here we have shown that the HspBP1 protein, the chaperone of the heat shock protein Hsp70, is able to form a complex with the previously discovered peptide (17.1) of the innate immunity protein Tag7. Experiments using thermophoresis demonstrated that the affinity of the Tag7 protein peptide 17.1 to the HspBP1 molecule is 100 times higher than that of the full-sized Tag7 molecule. The addition of the 17.1–HspBP1 complex to tumor cells induces apoptosis and necroptosis in them. The results obtained in this work can be used to develop promising antitumor drugs.