bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infectious Complications in Philadelphia-Negative Adolescent and Young Adult Acute Lymphoblastic Leukemia Patients in the Era of Pediatric-Inspired Regimen
Authors: Vongngakesone Phoutthasin , Niprapan Piangrawee , Srichairatanakool Sirichai , Hantrakun Nonthakorn , Punnachet Teerachat , Hantrakool Sasinee , Piriyakhuntorn Pokpong , Chai-Adisaksopha Chatree , Rattarittamrong Ekarat , Tantiworawit Adisak , Norasetthada Lalita , Rattanathammethee Thanawat 
Published: January 2025

Introduction: Pediatric acute lymphoblastic leukemia (ALL) has cure rates exceeding 90%, while adult ALL has a 3-year survival rate of only 40–50%. Adolescents and young adults (AYAs) face unique challenges due to age-related factors and high-risk disease features. Although pediatric-inspired regimens improve survival, intensive chemotherapy increases susceptibility to severe infections. Methods: A retrospective analysis was conducted on AYA-ALL patients treated at Chiang Mai University Hospital from 1 January 2007 to 31 December 2023. Patients were classified into pediatric-inspired (TPOG) or adult regimens (Hyper-CVAD or GMALL). Infections were categorized as clinically documented infections (CDI) or microbiologically documented infections (MDI). Multivariate logistic regression was used to identify infection risk factors. Results: Among 94 patients (62.8% male; median age 22.9 years), 56.4% received TPOG and 43.6% received adult regimens. Infectious complications occurred in 79.8% of patients, with higher rates in the adult regimen group (90.2% vs. 71.7%, p = 0.03). CDI was more frequent in the adult group (73.4% vs. 52.8%, p = 0.04), while fungal infections were more common in the TPOG group (26.4% vs. 9.8%, p = 0.04). Adult regimens significantly increased infection risk (OR 3.55; 95% CI 1.02–12.36, p = 0.04). Infection rates peaked during induction (47.8%) and consolidation (51.8%). During induction, MDI was lower in the adult group (21% vs. 79%, p = 0.01), while CDI was higher during consolidation (69% vs. 30%, p < 0.01). Gram-negative bacteria were the most common pathogens (85%), particularly E. coli (27%) and Salmonella spp. (21%). Fungal infections affected 19.1% of patients, with invasive pulmonary aspergillosis being the most common (11.7%). Conclusion: AYA-ALL patients are highly susceptible to infections, especially those receiving adult regimens. Fungal infections were notably more common in patients on the TPOG regimen. Strengthening infection prevention and providing early treatment are vital for improving patient outcomes.
fCUT&Tag-seq: An Optimized CUT&Tag-based Method for High-Resolution Histone Modification and Chromatin-Binding Protein Profiling in both Model and Plant Pathogenic Fungi
Authors: Wang Haiting , Tan Yongjunlin , Ma Jiayue , Yang Jie , Liu Mengran , Lu Shan , Xia Haoxue , Tang Guangfei , Liu Wende , Guo Hui-Shan , Shan Chun-Min 
Published: January 2025

AbstractHistone modifications and chromatin-binding proteins are important regulators of gene expression in eukaryotes and have pivotal roles in fungal pathogenicity and development. However, profiling these modifications or proteins across the genome in fungi is still challenging, due to the technical limitations of the traditional widely-used ChIP-Seq method. Here, we present an optimized CUT&Tag-Seq protocol (fCUT&Tag-Seq) specifically designed for filamentous fungi and dimorphic fungi. Our approach involves the preparation of protoplasts and nuclear extraction to enhance antibody accessibility, along with formaldehyde crosslinking to improve protein-DNA binding efficiency. We then successfully applied fCUT&Tag-Seq to accurately profile multiple histone modifications like H3K9me3, H3K27me3, H3K4me3, and H3K18ac, across different plant pathogenic or model fungal species includingVerticillium dahliae,Neurospora crassa,Fusarium graminearum, andSporisorium scitamineum. Compared to the traditional ChIP-Seq, our method showed superior signal-to-noise ratios, higher reproducibility, and enhanced detection sensitivity. Furthermore, we extended this method to profile chromatin-binding proteins, such as the histone acetyltransferase Gcn5. This study establishes fCUT&Tag-Seq as a robust and useful tool for fungal epigenetic research, enabling detailed exploration of chromatin dynamics and advancing our understanding of fungal gene regulation, development, and pathogenicity.
Connexin50 hemichannels are opened by CO2: implications for lens physiology
Authors: Lovatt Alexandra , Wijayapala Ashvini , Mui Macy , Butler Jack , Dale Nicholas 
Published: January 2025

AbstractConnexin50 (Cx50) is expressed in lens fibre cells. As mutations of Cx50 cause cataracts its physiological role in the lens must be important. We have used recent cryoEM structures of Cx50 and the predictive power of alphafold3 to identify the presence of a carbamylation motif, originally described in Cx26, that suggests that Cx50 might be CO2sensitive. By expressing the naturally truncated version of Cx50 in HeLa cells and utilising coexpression of genetically encoded sensors iGluSnFr or eLACCO1.1, we have demonstrated the CO2sensitivity of Cx50 hemichannels, and their permeability to lactate and glutamate. Importantly, the properties of Cx50 predict that it will be partially open at the levels of PCO2characteristic of aqueous humour. By mutating the two key residues of the carbamylation motif, K105 and K140, we have shown that the motif is required for CO2sensitivity. Mutations of the residue V44 cause cataracts and these mutations abolish the CO2sensitivity of Cx50. We hypothesize that CO2-dependent gating of Cx50, and consequent alterations in transmembrane Na+fluxes, might provide homeostatic control of the microcirculation system that is critical for lens health.
Co-opting MBNL-dependent alternative splicing cassette exons to control gene therapy in myotonic dystrophy
Authors: Carrell ST , Carrell EM , Giovenco RC , Davidson BL 
Published: January 2025

AbstractMyotonic dystrophy type 1 (DM1) is a multisystemic genetic disorder caused by a CTG repeat expansion that accumulate as toxic CUG repeat RNA. Functional sequestration of muscleblind-like (MBNL) proteins by CUG repeat RNA leads to deleterious, yet predictable changes in alternative splicing in DM. Genetic medicines for DM that reduce CUG repeat RNA or increase MBNL are advancing, but application of a viral-based approach must contend with high phenotypic and molecular variability. To address this, we have repurposed well-described cassette exons that are excluded by MBNL action to tune translational output of a therapeutic protein. We show that these splicing events can be taken out of genetic context and respond to changes in MBNL concentration or accumulation of toxic CUG repeat RNA, can deliver therapeutic MBNL1 protein to improve skeletal muscle myotonia or prevent cardiac toxicity associated with MBNL1 overexpression in mice, and distinguish DM patient-derived skeletal muscle myotubes from isogenic controls. Further work to fine-tune events to specific tissue targets and therapeutic cargos is needed, but these events can increase the therapeutic window for viral-based approaches for DM1.
Functional impact of rare variants and sex across the X-chromosome and autosomes
Authors: Ungar Rachel A. , Li Taibo , Vetr Nikolai G. , Ersaro Nicole , Battle Alexis , Montgomery Stephen B. 
Published: January 2025

AbstractThe human X-chromosome contains hundreds of genes and has well-established impacts on sex differences and traits. However, the X-chromosome is often excluded from many genetic analyses, limiting broader understanding of variant effects. In particular, the functional impact of rare variants on the X-chromosome is understudied. To investigate functional rare variants on the X-chromosome, we use observations of outlier gene expression from GTEx consortium data. We show outlier genes are enriched for having nearby rare variants on the X-chromosome, and this enrichment is stronger for males. Using the RIVER model, we identified 753 rare variants in 449 genes predicted to have functional differences between males and females. We examined the pharmacogenetic implications of these variants and observed that 25% of drugs with a known sex difference in adverse drug reactions were connected to genes that contained a sex-biased rare variant. We further identify that sex-biased rare variants preferentially impact transcription factors with predicted sex-differential binding, such as the XIST-modulated SIX1. Combined, our study investigates functional rare variants on the X-chromosome, and further details how sex-stratification of variant effect prediction improves identification of rare variants with predicted sex-biased effects, transcription factor biology, and pharmacogenomic impacts.
Data-driven denoising in spinal cord fMRI with principal component analysis
Authors: Hemmerling Kimberly J. , Vigotsky Andrew D. , Glanville Charlotte , Barry Robert L. , Bright Molly G. 
Published: January 2025

ABSTRACTNumerous approaches have been used to denoise spinal cord functional magnetic resonance imaging (fMRI) data. Principal component analysis (PCA)-based techniques, which derive regressors from a noise region of interest (ROI), have been used in both brain (e.g., CompCor) and spinal cord fMRI. However, spinal cord fMRI denoising methods have yet to be systematically evaluated. Here, we formalize and evaluate a PCA-based technique for deriving nuisance regressors for spinal cord fMRI analysis (SpinalCompCor). In this method, regressors are derived with PCA from a noise ROI, an area defined outside of the spinal cord and cerebrospinal fluid. A parallel analysis is used to systematically determine how many components to retain as regressors for modeling; this designated a median of 11 regressors across three fMRI datasets: motor task (n=26), breathing task (n=27), and resting state (n=10). First-level fMRI modeling demonstrated that principal component regressors did fit noise (e.g., physiological noise from blood vessels), particularly in the resting state fMRI dataset. However, group-level motor task activation maps themselves did not show a clear benefit from including SpinalCompCor regressors over our original denoising model. The potential for collinearity of principal component regressors with the task may be a concern, and this should be considered in future implementations for which task-correlated noise is anticipated.
A single dimer of the SARS-CoV-2 N protein can associate with multiple fragments of single-stranded and stem-loop RNAs
Authors: Kaneda Naoya , Suzuki Leo , Endo Shun , Husna Syamil Muharror Ahsanul , Ishikawa Shion , Takahashi Hiroto , Oikawa Hiroyuki , Itoh Yuji , Takahashi Satoshi 
Published: January 2025

AbstractThe nucleocapsid (N) protein of SARS-CoV-2 associates with the viral genomic RNA (gRNA) and forms the ribonucleoprotein (RNP) granules. However, the detailed molecular structures of RNP and their formation mechanism are largely unknown. We used circular dichroism (CD) spectroscopy, fluorescence correlation spectroscopy (FCS) and single-molecule Förster resonance energy transfer (sm-FRET) spectroscopy to understand the interaction between the N protein and different structural units of RNA. We chose polyadenylate chains with 40, 30 and 20 bases possessing a single-stranded structure and three stem loops with 50, 41 and 29 bases selected from gRNA, and labeled their 5’ and 3’ ends by Alexa488 and Alexa647, respectively, for the FCS and sm-FRET measurements. We found that the N protein started to bind to the single-stranded RNAs at the concentrations between 10 and 100 nM. The binding of the N protein to one of the stem loops occurred at the concentration less than 10 nM without melting the stem loop. For all the samples, the binding of multiple molecules of the RNA fragments to a single dimer of the N protein was observed. These results demonstrate that the N protein acts as a non-specific binder to both single-stranded and stem-loop structures of RNA, and that the N protein might contract a long RNA chain by bridging its multiple segments. We propose that the RNP granules might be folded by the association of the numerous stem loops of gRNA triggered by the assembly of the N protein.
Patching up the nucleus: a novel role for PMLII in nuclear envelope rupture repair
Authors: Janssen Anne F.J. , Knowles Oliver , Britton Sébastien , Deane Janet E. , Spruijt Evan , Larrieu Delphine 
Published: January 2025

AbstractThe nuclear envelope (NE) is important for cellular health as it protects and organizes the genome. NE dynamics is important for various cellular processes including cell growth, migration and removal of defective NE components. In extreme cases, the NE can rupture leading to exchange of material between the nuclear interior and the cytoplasm. Rapid repair of the NE is initiated to minimize the effect on the genome. While our understanding of the machinery involved in this repair process is increasing, a lot is still unknown about this process including events leading up to NE rupture. Interestingly, biomolecular condensates have recently been found to play important roles in membrane repair and remodelling in cells. Here, we found that promyelocytic leukemia protein isoform II (PMLII), a protein involved in nuclear PML body formation, forms condensates at the NE. These condensates specifically form at sites where the lamina is disrupted. We show that NE rupture often occurs at these sites and that PMLII stays present until rupture repair is initiated suggesting a role in stabilization of the site for effective repair.
Hunting for a charged Higgs boson pair in proton-proton collisions
Authors: Arroyo-Ureña M. A. , Herrera-Chacón E. A. , Rosado-Navarro S. , Salazar Humberto 
Published: January 2025

We explore the production and possible detection of a charged scalar Higgs pair H−H+ decaying into the final state μνμcb in proton-proton collisions at the LHC and its next step, the high luminosity LHC (HL-LHC). The charged scalars are predicted within the theoretical framework of the two-Higgs doublet model of type III (2HDM-III). As a test and validation of the model, we identify regions of the 2HDM-III parameter space that accommodate the current excess of events at 3σ in the process BR(t→H±b)×BR(H±→cb) for MH±=130  GeV, as reported by ATLAS Collaboration. Theoretical and additional experimental constraints are also included. Based on this, we propose realistic scenarios that could be brought under experimental scrutiny at the HL-LHC. Assuming the most favorable scenario, we predict a signal significance at the level of 5σ for a charged scalar boson mass MH± in the 100–350 GeV range.

Published by the American Physical Society
2025
Assessing the structural relationships between the CIPP model components in teacher education program
Authors: Alquraan Mahmoud Fisal , Alazzam Sulaf , Farhat Dima 
Published: January 2025

PurposeThe objective of this study is to explore the structural relationships among context (C), input (I) and process (P) and product (P) (CIPP) components of teacher preparation programs based on students' perceptions.Design/methodology/approachIn this study, data were collected using a 17-item scale. The study sample consisted of 213 pre-service teachers enrolled in the Postgraduate Professional Diploma in Teaching (PPDT). Quantitative research methodology with multivariate structural equation modeling (SEM) was utilized to examine the two suggested models.FindingsThe results of this study show that the CIPP model can be projected into preservice teachers' perceptions of the CIPP components. These preservice teachers' perceptions of preparation programs from the three components (CIP) can predict preservice teachers' perceptions of teachers’ preparation program products or outcomes (i.e. the fourth CIPP component). This result indicates that the relationships between the CIPP components and the pre-service teachers' perceptions of the Diploma in Teaching program are direct.Originality/valueTwo suggested models were tested to explore the structural relationships between CIPP components. The first model represents the original CIPP model with indirect relationships between the four components: CIPP. The second model suggests direct relationships between the first three components (CIP) and the objectives or products (P).
Understanding career-meaningfulness and work centrality to serve customers: servant leadership and customer-oriented boundary-spanning behaviors
Authors: Saeed Munazza , Bani-Melhem Ahmad Jamal , Hassan Hamid , Alvi Tariq Hameed , Altaf Saira 
Published: January 2025

PurposeDrawing on the conservation of resources theory, this research examines the relationship between managers’ servant leadership and frontline employees’ customer-oriented boundary-spanning behaviors by considering career meaningfulness as an underlying mechanism. Furthermore, this study investigates a moderated mediation model by proposing work centrality as a boundary condition in the relationship between career meaningfulness and customer-oriented boundary-spanning behaviors.Design/methodology/approachTwo-source (manager-frontline employee dyad) data were collected through a survey questionnaire from hospitality organizations at three different points in time. Hayes’ PROCESS macro was used to analyze the data.FindingsFindings show that managers’ servant leadership fosters frontline employees’ customer-oriented boundary-spanning behaviors directly and through career meaningfulness. Frontline employees’ work centrality moderates the relationship between career meaningfulness and customer-oriented boundary-spanning behaviors (second stage) and the indirect effect of servant leadership (through career meaningfulness) on customer-oriented boundary-spanning behaviors (moderated mediation).Practical implicationsThe findings imply that managers should exhibit servant leadership behaviors to enhance frontline employees’ sense of career meaningfulness and customer-oriented boundary-spanning behaviors. Additionally, hospitality organizations and managers are encouraged to prioritize work centrality when hiring frontline employees and implement training programs to cultivate work centrality.Originality/valueThis study’s originality lies in exploring career meaningfulness as the underlying mechanism linking servant leadership to customer-oriented boundary-spanning behaviors while also examining work centrality as a second-stage moderator in this relationship.
Evaluation of the operational efficiency of China’s intellectual property public information service platform
Authors: Wanyan Dengdeng , Liu Chenyu 
Published: January 2025

Purpose
The purpose of this paper is to measure the operational efficiency of China’s intellectual property public information service (IPPIS) platform, discuss the current problems of the operational efficiency of China’s IPPIS platform and put forward suggestions for improvement.

Design/methodology/approach
First, the paper chose 36 Chinese IPPIS platforms as the research object and established an assessment indicator system for the operational efficiency of IPPIS platforms that includes six input indicators and three output indicators. Second, the authors used Data Envelopment Analysis (DEA) method to calculate the operational efficiency of 36 Chinese IPPIS platforms. Finally, the authors analyze the problems that exist in the operational efficiency of China’s IPPIS platform and put forward suggestions for improvement.

Findings
Of the 36 platforms measured, only 9 are in DEA effective status, which means operating well. In addition, this study finds that the following problems exist in China’s IPPIS platforms: the overall operational efficiency is low, and there are large geographic differences; a small number of platforms have low scale efficiency; and half of the platforms have high input redundancy and output insufficiency in different indexes.

Originality/value
This paper aims to help the platform constructor to provide suggestions on the direction and extent of improvement. In addition, this study is expected to raise the attention of the management and practice departments of other external countries (especially developing countries) to the operational efficiency of IPPIS platforms and to provide experiences for other external countries to improve their platform construction.
Examining the brand-level associations between customer satisfaction, demographic profile and loyalty
Authors: Dawes John G. 
Published: January 2025

PurposeThis study examines the extent to which brand-level satisfaction scores are related to loyalty metrics, controlling for the double jeopardy effect as well as the demographic profile of the brand’s customer base.Design/methodology/approachThe study uses data for brands in three UK financial services categories: banks, car/home insurance, and life insurance. Regression analysis is used to examine the relationships between brand size, satisfaction levels, demographic profiles, and loyalty.FindingsFirstly, the study finds a strong “double jeopardy” association. That is, larger brands have more loyalty, both in terms of behavior and stated preference. Next, brands with higher satisfaction scores tend to have somewhat higher first-preference loyalty, controlling for the double jeopardy effect. There are mixed results in relation to satisfaction’s link to behavioral loyalty. Lastly, aspects of a brand’s demographic profile, particularly in terms of whether it skews towards high-income customers, are associated with somewhat lower loyalty metrics, both behavioral and conative.Originality/valueThe findings represent an original contribution by translating what have been to date principally individual-buyer level associations (between satisfaction, demographics and loyalty) into brand-level relationships that are arguably more relevant to managers who act on this level of reporting.
Dietary restrictions and special food needs in tourism contexts: does the love of events go through the stomach?
Authors: Pompurová Kristína , Šimočková Ivana , Marčeková Radka , Šebová Ľubica , Lazurová Izabela 
Published: January 2025

PurposeThis study aims to investigate whether and how dietary restrictions or special dietary needs, arising not only from health concerns but also from lifestyle or religious considerations, impact the experiences of visitors at various organized events.Design/methodology/approachThe study employs a qualitative research design, interpreting results of both content and sentiment analysis of 154 in-depth interviews with participants from cultural, sports and other events, originating from 18 countries and having dietary restrictions due to health, lifestyle or religious considerations.FindingsThe results underscore that the impact of dietary restrictions and special dietary needs on organized events depends on the duration and nature of the event. This significance is particularly pronounced in events lasting several hours or more and those centred around food and beverages, such as gastronomic festivals. While individuals with long-term dietary restrictions exhibit effective coping mechanisms without compromising their event experience, those with short-term restrictions feel particularly restricted. Although the provision of non-inclusive food during events may not immediately lead to participant dissatisfaction, it has the potential to impact the event’s success in the future.Originality/valueThis study is the first to investigate a sufficiently large sample of interviewees with diverse dietary restrictions, encompassing more than just health-related reasons, who attend various types of events in tourist destinations. It is also the first to employ sentiment analysis to better understand the impact of dietary restrictions on visitor experiences at events. This research paves the way for further studies in this field, emphasizing specific event types and different visitor groups.