bluuebunny/crossref_metadata_2025_split huggingface dataset:
S2AI pelvic fixation is an important factor affecting sagittal spinal parameters in ADS patients in the short term after surgery: a retrospective study
Authors: Wang Xin-yu , Xi Jian-wei , Liu Lei , Han Meng , Liu Guang-pu , Zhang Jin , Ma Chao 
Published: October 2023

Abstract
Background
The incidence of degenerative scoliosis has increased significantly with the exacerbation of population aging, and there are many doubts about the methods and results of pelvic fixation for degenerative scoliosis. This study aimed to investigate the short-term changes in sagittal parameters of the spine after bilateral S2-ALAR-ILIAC (S2AI) screw fixation combined with posterior long-segment spinal fusion and internal fixation in ADS patients.
Methods
A total of 25 ADS patients who underwent bilateral S2AI internal fixation combined with posterior long-segment orthopedic surgery in Xuzhou Central Hospital from January 2018 to December 2022 were included in the retrospective analysis. The preoperative and postoperative Cobb angle, sagittal balance (SVA), pelvic incidence angle (PI), lumbar lordosis angle (LL), the difference between pelvic projection angle and lumbar lordosis angle (PI-LL), VAS score, ODI index, and JOA score were compared.
Results
All patients were followed up for at least 6 months. The mean Cobb, SVA, PI, LL, PI-LL were (42.67 ± 17.36)°,(70.44 ± 26.09) mm, (51.53 ± 7.67)°, (25.62 ± 13.27)°, (26.33 ± 10.44)°, (27.20 ± 9.56)°, respectively, the mean VAS score was (3.08 ± 0.28), the mean JOA score was (11.72 ± 1.10) and the mean ODI index was (63.08 ± 6.01)%. At 6 months postoperatively, mean scoliosis Cobb was (17.60 ± 7.19)°, mean SVA was (23.16 ± 12.39) mm, mean PI was (48.45 ± 5.42)°, mean LL was (38.01 ± 6.43)°, mean PI-LL was (10.45 ± 4.33)°, mean PT was (21.20 ± 6.32)°, mean VAS score was (8.64 ± 0.79) points, mean JOA score was (11.72 ± 1.10) points, and mean ODI index was (20.76 ± 2.74) %, with statistically significant differences (P < 0.05).
Conclusion
The clinical effect of bilateral S2AI technology combined with posterior long-segment fixation in treating ADS is significant and can effectively restore the sagittal sequence of the spine. However, careful correction of excessive lordosis should be made in the preoperative design to avoid PI-LL mismatch resulting in decreased postoperative quality of life.
Strategies to implement evidence-informed practice at organizations: A rapid systematic review
Authors: Clark Emily C , Burnett Trish , Blair Rebecca , Traynor Robyn L , Hagerman Leah , Dobbins Maureen 
Published: October 2023

Abstract
Background
Achievement of evidence-informed decision making (EIDM) requires the integration of evidence into all practice decisions by identifying and synthesizing evidence, then developing and executing plans to implement and evaluate changes to practice. Evidence-informed practice (EIP) involves implementing a specific practice or program with proven effectiveness. This rapid systematic review examines strategies for the implementation of EIDM and EIPs across organizations, mapping facilitators and barriers to the COM-B (capability, opportunity, motivation, behaviour) model for behaviour change.
Methods
A systematic search was conducted in multiple databases and by reviewing publications of key authors. Articles that describe interventions to shift teams, departments, or organizations to EIDM or EIP were eligible for inclusion. For each article, quality was assessed, and details of the intervention, setting, outcomes, facilitators and barriers were extracted from each included article. A convergent integrated approach was undertaken to analyze both quantitative and qualitative findings.
Results
Fifty-nine articles are included. Studies were conducted in primary care, public health, social services, occupational health, and palliative care settings. Strategies to implement EIDM and EIP included the establishment of Knowledge Broker-type roles, building the EIDM capacity of staff, and research or academic partnerships. Facilitators and barriers align with the COM-B model for behaviour change. Facilitators for capability include the development of staff knowledge and skill, establishing specialized roles, and knowledge sharing across the organization, though staff turnover and subsequent knowledge loss was a barrier to capability. For opportunity, facilitators include the development of processes or mechanisms to support new practices, forums for learning and skill development, and protected time, and barriers include competing priorities. Facilitators identified for motivation include supportive organizational culture, expectations for new practices to occur, recognition and positive reinforcement, and strong leadership support. Barriers include negative attitudes toward new practices, and lack of understanding and support from management.
Conclusion
This review provides a comprehensive, in-depth analysis of facilitators and barriers for the implementation of EIDM and EIP in public health and related organizations, mapped to the COM-B model for behaviour change. The facilitators and barriers described in the included studies establish key factors for realizing greater implementation success in the future.
Registration
PROSPERO CRD42022318994
General allometric equation for estimating aboveground biomass of Acacia-Commiphora forest, southern Ethiopia
Authors: Amanuel Wondimagegn , Tadesse Challa , Molla Moges , Getinet Desalegn , Mekonnen Zenebe 
Published: October 2023

Abstract

Most of the biomass equations were developed using sample trees collected mainly from pan-tropical and tropical regions that may over- or underestimate biomass. Site-specific models would improve the accuracy of the biomass estimates and enhance the country’s MRV activities. The aim of the study is to develop site & species-specific biomass estimation models and validate and evaluate the existing generic models developed for pan-tropical forest and newly developed allometric models. Total of 150 individual sample trees was harvested from each diameter class (proportionally) for the site-specific biomass model development. Data was analyzed using SAS procedures. All relevant statistical tests (normality, multicollinearity and heteroscedasticity) were performed. Data was transformed to logarithmic functions and multiple linear regression techniques were used to develop model to estimate aboveground biomass. The root mean square error (RMSE) was used for measuring model bias, precision and accuracy. The coefficient of determination (R2 & adj-R2), the Akaike Information Criterion (AIC) and the Schwarz Bayesian information Criterion (SBC) was employed to select most appropriate models. The results showed that a best fit-model for above-ground biomass of tree components (stem, branch and foliage) was developed.
AGBStem = exp {-1.8296 + 0.4814 Ln (ρD2H) + 0.1751 Ln (CW) + 0.4059 Ln (DSH30)}
AGBBranch = exp {-131.6 + 15.0013 Ln (ρD2H) + 13.176 Ln (CW) + 21.8506 Ln (DSH30)}
AGBFoliage = exp {-0.9496 + 0.5282 Ln (DSH30) + 2.3492 Ln (ρ) + 0.4286 Ln (CW)}
AGBTotal = exp {-1.8245 + 1.4358 Ln (DSH30) + 1.9921 Ln (ρ) + 0.6154 Ln (CW)}
The results of this study demonstrate that the development of local models derived from an appropriate sample of representative species can greatly improve the estimation of AGBTotal.
Raman spectroscopy of lymphocytes from patients with the Epstein-Barr virus infection
Authors: Pietruszewska Magdalena , Biesiada Grażyna , Czepiel Jacek , Birczyńska-Zych Malwina , Moskal Paulina , Garlicki Aleksander , Wesełucha-Birczyńska Aleksandra 
Published: October 2023

Abstract
In this study, Raman spectroscopy was applied to trace lymphocytes activation following contact with the Epstein-Barr virus (EBV) of the herpesvirus family. The biomarker of cell activation was found to be the 520 cm− 1 band, indicating formation of immunoglobulins. The blood samples were obtained from patients diagnosed with infectious mononucleosis and treated at the University Hospital in Kraków. The lymphocytes’ Raman spectra were collected using a mapping technique, exciting samples with a 514.5 nm line of Ar + laser. Measurements were performed on the 1st, 4th, 6th, 12th and 30th day of hospitalization, until the patient recovered. The highest intensity of the immunoglobulin marker was observed on the 4th day of hospitalization, while the results of the blood count of patients showed the greatest increase in the number of lymphocytes at the beginning of hospitalization. The blood of the healthy volunteers lacked activated lymphocytes. Some information was provided by the evaluation of B-cell activation by estimating the activated areas in the cells, which were determined by the presence of the Ig marker. The 900 cm− 1 band and band around 1450 cm− 1 were also analyzed as markers of the presence of the latent membrane protein, LMP2A (and 2B), of the EBV viral protein. The anomalous degree of depolarization observed in B-cells in the course of EBV infection appears to be due to the influence of a virus protein, disrupting BCR signal transduction.
Neuroprotective effects of CysLT2R antagonist on Angiostrongylus cantonensis-induced edema and meningoencephalitis
Authors: Chen Ke-Min , Lai Shih-Chan 
Published: October 2023

Abstract

Background
The pathogenesis of Angiostrongylus cantonensis-induced eosinophilic meningoencephalitis includes haemorrhage, brain edema formation, disrupting the blood–brain barrier (BBB), and induction of an inflammatory response. Cysteinyl leukotrienes (CysLTs) can induce a disruption of the BBB, and this reaction is mediated by cysteinyl-leukotriene receptors. In this study, we used A. cantonensis-induced eosinophilic meningoencephalitis as a model to investigate whether the CysLT2 receptor involved in the pathogenesis of angiostrongyliasis meningoencephalitis.
Methods
The brain edema was determined using the wet weight/dry weight method. Microglia polarization was detected by Flow cytometry and Enzyme-linked immunosorbent assay. Evans blue method was used to measure changes in the blood brain barrier, while western blotting was used to analyze BBB-related proteins. Gelatin zymography was used to assay matrix metalloproteinase-9 (MMP-9). MicroRNA expression was detected by Quantitative reverse transcription-PCR (qRT-PCR).
Results
The present study provides evidence that the CysLT2 receptor antagonist HAMI3379 reduced the number of infiltrated eosinophils and brain edema in eosinophilic meningoencephalitis. Additionally, we found that HAMI3379 significantly decreased the protein levels of M1 polarisation markers (CD80, iNOS, IL-5 and TNF-α), increased the expression of M2 polarisation markers (CD206, IL-10 and TGF-β) both in vivo and in vitro. Matrix metalloproteinase-9, S100B, GFAP, fibronectin, and claudin-5 were markedly lower after HAMI3379 treatment. Therefore, HAMI3379 reduced the BBB dysfunction in angiostrongyliasis meningoencephalitis. We have identified microRNA-155 as a BBB dysfunction marker in eosinophilic meningoencephalitis. The results showed that microRNA-155 was 15-fold upregulated in eosinophilic meningoencephalitis and 20-fold upregulated after HAMI3379 treatment.
Conclusions
Our results suggest that CysLT2R may be involved in A. cantonensis-induced brain edema and eosinophilic meningoencephalitis and that down-regulation of CysLT2R could be a novel and potential therapeutic strategy for the treatment of angiostrongyliasis meningoencephalitis.
A convenient and improved selection method for immortalisation of neural cells
Authors: Zheng Jian , Yao Yingling , He ling , Zhang Yanting , Bi Yang , Nan Guoxin 
Published: October 2023

Abstract
There is a wide variety of cells in the nervous system that collaborate with each other for information transfer, regulation and neuroprotection. Various cell investigations involving primary cells isolated from mammalian tissues are helpful in understanding disease pathophysiology. However, these cells are difficult to acquire in vitro in a short time and die of aging after repeated passages. These hurdles have hindered cell and disease research. Immortalized cell lines are necessary for cell research. After creating immortalized cell models, antibiotics are commonly employed to select immortalized cells. However, immortalized genes have poor transfection rates and a considerable percentage of non-immortalized cells are destroyed, shed, or are not harvested from suspension following antibiotic selection. Cells form a network of a variety of cell junctions. Consequently, when a large number of cells die and fall off, immortalized cells that were successfully transfected and had antibiotic resistance can also be removed, leaving only a small number of viable immortalized cells. Because of reciprocal nourishment between cells, when a few survive, their proliferation ability is also limited due to a lack of other cell secretion factors. Thus, obtaining immortalized cell lines takes more time. In this study, cells with transfected immortalized genes were cultured, subcultured, and selected. This process could generate stable immortalized cell lines in less time by enhancing the classic immortalized cell selection procedure. The feasibility and advantages of this method were demonstrated by infecting Schwann cells with retroviruses containing plasmid SSR#69 packaged in HEK293 cells. The resulting immortalized Schwann cell lines were compared to cells obtained by the standard screening before culture.
Identification of Potential Diagnostic and Prognostic Biomarkers for Cervical Cancer
Authors: Zhao Jinfei , Xia Xuemei , Dong Mimi 
Published: October 2023

Abstract

ObjectiveTo explore potential diagnostic and prognostic markers of cervical cancer by using GEO and TCGA databases.MethodsExpression matrices related to cervical cancer were downloaded from the GEO database. Gene expression and clinical-pathological data from TCGA and GTEx were obtained from the UCSC Xena database. Differentially expressed genes (DEGs) between normal and tumor tissue samples were identified using the limma package in R. DEGs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses using the ClusterProfiler package. The Cox proportional hazard regression model was used to screen significant genes. ROC curve and multivariate Cox regression analysis were used to evaluate the prognostic value of multiple clinical features.ResultsIn this study, 42 total DEGs were found, including 33 up-regulated genes and 9 down-regulated genes. GO analysis revealed that DEGs were involved in biological processes such as chromosomal segregation, nuclear division, and organelle fission. KEGG pathway analysis implicated Toll-like receptor and mismatch repair signaling pathways. 6 significant genes were identified by COX (p < 0.05) and CA9, GINS2, and SPP1 combined biomarkers divided cervical cancer patients into a high-risk group and a low-risk group. Moreover, the low-risk survival rate was significantly higher than the high-risk survival rate. Finally, multivariate Cox regression analysis showed that the combined biomarkers of CA9, GINS2, and SPP1 are independent predictors of the prognosis of cervical cancer patients.ConclusionThe GEO and TCGA databases screened out the combined biomarkers of CA9, GINS2, and SPP1, which are independent prognostic predictors of cervical cancer.
The use of multiple stent retrievers in emergent endovascular procedures; its predictors, associated costs and patient outcomes
Authors: Amankwah Curtis , Lombardo Lauren , Rutledge John , Sattar Ahsan , Chancellor Bree , Altschul Dorothea 
Published: October 2023

Abstract
Background and Purpose
The average annual cost of stroke treatment is over $40 billion in USA. With recent advancement of tools used in thrombectomies, the question of whether the use of multiple costly devices in an emergent thrombectomy will lead to a better or worse outcome when compared to simpler devices remains unanswered.
Materials and Methods
We retrospectively analyzed 89 patients who underwent emergent stroke interventions at a community hospital from January 2018 to February 2021. Patients were grouped into Aspiration only (ASP) n = 15, 1 Stent-retriever (SR) + 1ASP n = 59, and ≥ 1SR + ≥ 1ASP n = 15, based on main devices/device combination used that affected cost. Demographic and perioperative variables were assessed by multivariate analysis to determine possible predictors and outcomes associated with multiple device use (≥ 1SR + ≥ 1ASP).
Results
The multiple device/SR group had the highest mean total cost of devices per procedure (~$21,104) when compared to No SR/ASP only (~$4,477) and 1SR + 1ASP (~$12,295) groups. We identified LSW to puncture time (OR = 1.001; 95% CI 1.000 to 1.003, p = 0.05), and NIHSS score (OR = 1.085; 95% CI 1.085 to 1.173, p = 0.04) as significant predictors of multiple device use. Regarding outcomes, the use of multiple SR was significantly associated with poor/incomplete recanalization rates (OR = 0.191, 95% CI 0.049 to 0.741, p = 0.017) and functional dependence mRS 4–6 (OR=-1.02; 95% CI -2.04 to -0.01, p = 0.048).
Conclusions
After an unsuccessful initial attempt with a single device to achieve complete revascularization in EVTs, the additional use of stent-retrievers leads to unimproved recanalization rates at an increased cost with no additional clinical benefit to the patients. This was especially seen in patients who presented in the delayed time window (> 360 min) with high NIHSS scores.
Whole-brain connections of glutamatergic neurons in the lateral habenula in both sexes
Authors: Huang Hongren , Liu Xue , Wang Liping , Wang Feng 
Published: October 2023

Abstract
Background The lateral habenula (LHb) is an evolution-conserved epithalamic nucleus that plays a role in multiple physiological functions, such as encoding value signals, integrating emotional information, and regulating related behaviors. The cells within the LHb are predominantly glutamatergic and have heterogeneous functions in response to different stimuli. In addition, the circuitry connections of the LHb glutamatergic neurons anchor a vast array of event integration. However, circuitry connections of LHb glutamatergic neurons in both sexes have yet to be investigated.
Methods In this study, we microinjected Cre-dependent retrograde trace virus and anterograde synaptophysin-labeling virus into the LHb of adult male and female Vglut2-ires-Cre mice respectively and quantitatively analyzed the ipsilateral and contralateral whole-brain input as well as output of the LHb glutamatergic connections.
Results We found that the afferent of the LHbvGlut2 neurons involved more than 30 brain subregions, arising from the cortex, striatum, pallidum, thalamus, hypothalamus, midbrain, pons, medulla, and cerebellum without presenting significant sex differences. Additionally, the efferent of the LHbvGlut2 neurons consisted of eight parts of large brain regions, mainly targeting midbrain and pons nuclei, with distinct presynaptic bouton features across each brain subregion. Although correlation and cluster analysis revealed a different input and collateral projection feature, the input-output connection pattern of LHbvGlut2 neurons in both sexes was highly similar.
Conclusions This study systematically and comprehensively analyzed the whole brain input and output connections of the LHbvGlut2 neurons in male and female mice, providing insights into the anatomical architecture of the mouse cell-type-specific LHb neurons. This structural basis can guide investigations into the complex functions of the LHb.
Experimental Anaysis on the Effects of Trace Metals as Micronutrients in Enhancing Biomethane Production
Authors: C Tintu Mary , K Swarnalatha , J Harishma S. 
Published: October 2023

Abstract
Anaerobic digestion (AD) of microbial biomass has proven to be a significant breakthrough technique in producing biogas rich in methane. The quantity of biogas produced by anaerobic digestion processes varies significantly based on the nature and characteristics of substrates used. Although various substrates have been studied to produce biogas rich in biomethane by various methods, studies using micronutrients for enhancing biomethane production are less explored. This research work focusses the use of trace metals such as Fe, Cu, Zn, Mn, Mg, Ni in proper proportions to enhance the microbial consortium thus aiding in the production of methane of desired quality. Initial parameter analysis of the samples was done based on specific dosages of the micronutrients supplied to the reactor. The effect of these micronutrients on the anaerobic digestion process was observed by analysing the biogas yield, pH, alkalinity, total solids, and volatile solids of the samples. Studies were done on daily basis in triplicate samples to arrive at concordant results. Once the minimum dosage was formulated the nutrient supplementation was done on varying dosages for improving the biomethane potential of the samples. Food waste from the college canteen was used as the substrate and cow dung was used as an inoculum for providing a catalytic effect in the anaerobic reactor. An energy balanced microbial reactor set up was developed for varying proportions of metals used as micronutrients. Further studies showed a drastic decrease in Volatile Fatty Acids (VFA) accumulation in the anaerobic reactor with increase in retention period which proves the stability of the system in the long run. The results obtained from this study shows an effective method for conversion of waste to energy in less time and in achieving sustainable development goals for treating waste.
Archaeological cognition of the Eastern Mausoleum of Qin State using integrated space-ground observation tools
Authors: Luo Yansong , Chen Fulong , Gao Sheng , Zhu Meng , Zhou Wei , Elfadaly Abdelaziz 
Published: October 2023

Abstract
As a significant component of the Qin Dynasty's royal tombs, the ruins from the Eastern Mausoleum of the Qin State would be mirrored to present the social development level during the Warring States period (475 BC ~ 221 BC) in China. In this study, we applied multi-mode space-ground observations and derivative Digital Elevation Models to assess the plausibility of Remote Sensing (RS) in archaeology and to uncover the hitherto poorly understood conditions/situations for heritage conservation. We first applied the ‘Normalized Difference Vegetation Index’ and the ‘Normalized Difference Moisture Index’ to highlight archaeological traces. Additionally, for the first time, we proposed utilizing thermal infrared band data from the SDGSAT-1 satellite for the exploration of thermal archaeological traces, revealing an enhancement in archaeological signs. Subsequently, we use electromagnetic prospecting to confirm the presence of an ancient tomb passage. The landscape analysis reveals the hold of the Chinese Geomancy Theory on the site selection for royal tombs, this philosophy in turn is significant for heritage conservation from the natural force of surface erosion. The RS-based change detection using CORONA and Google Earth images reveals negligible surface erosion traces along with the mitigated anthropogenic disturbance. This study implies the potential of multi-mode space-ground observations for the cultural heritage archaeological value exploitation as well as the sustainable conservation of these irreplaceable properties.