bluuebunny/crossref_metadata_2025_split huggingface dataset:
On working with poison: Reflections on painful empowerment in queer faculty‐student participatory action research
Authors: Lichty Lauren F. , Belmont Jessica 
Published: October 2023

AbstractHuman sexuality textbooks, like most social and health sciences products, are notoriously limited in their inclusion of queer, trans, and intersex people. While well‐intentioned faculty (like the first author) do their best to address these limitations, sometimes it isn't enough. Sometimes our texts, and we, cause harm. This paper describes two phases of a participatory action research (PAR) project involving queer, trans, and intersex students and a queer, nonbinary faculty member that intended to address harm tied to a course text, support student empowerment, and move toward action to improve unjust textbook representation. Through first‐person reflective storytelling, we, the faculty member and one student member of the research team, share our approach to “working with poison,” including strategies for infusing trauma‐informed practices into our PAR approach. We reflect on the pain of doing this work, and the ways our approach succeeded and failed. We end with recommendations for individuals, publishers, and institutions looking to minimize harm and promote justice in higher education curriculum.
A newly identified ferritin L‐subunit variant results in increased proteasomal subunit degradation, impaired complex assembly, and severe hypoferritinemia
Authors: Shagidov Dayana , Guttmann‐Raviv Noga , Cunat Séverine , Frech Liora , Giansily‐Blaizot Muriel , Ghatpande Niraj , Abelya Gili , Frank Gabriel A. , Aguilar Martinez Patricia , Meyron‐Holtz Esther G. 
Published: October 2023

AbstractFerritin is a hetero‐oligomeric nanocage, composed of 24 subunits of two types, FTH1 and FTL. It protects the cell from excess reactive iron, by storing iron in its cavity. FTH1 is essential for the recruitment of iron into the ferritin nanocage and for cellular ferritin trafficking, whereas FTL contributes to nanocage stability and iron nucleation inside the cavity. Here we describe a female patient with a medical history of severe hypoferritinemia without anemia. Following inadequate heavy IV iron supplementation, the patient developed severe iron overload and musculoskeletal manifestations. However, her serum ferritin levels rose only to normal range. Genetic analyses revealed an undescribed homozygous variant of FTL (c.92A > G), which resulted in a Tyr31Cys substitution (FTLY31C). Analysis of the FTL structure predicted that the Y31C mutation will reduce the variant's stability. Expression of the FTLY31C variant resulted in significantly lower cellular ferritin levels compared with the expression of wild‐type FTL (FTLWT). Proteasomal inhibition significantly increased the initial levels of FTLY31C, but could not protect FTLY31C subunits from successive degradation. Further, variant subunits successfully incorporated into hetero‐polymeric nanocages in the presence of sufficient levels of FTH1. However, FTLY31C subunits poorly assembled into nanocages when FTH1 subunit levels were low. These results indicate an increased susceptibility of unassembled monomeric FTLY31C subunits to proteasomal degradation. The decreased cellular assembly of FTLY31C‐rich nanocages may explain the low serum ferritin levels in this patient and emphasize the importance of a broader diagnostic approach of hypoferritinemia without anemia, before IV iron supplementation.
Ortho‐Alkoxy‐benzamide Directed Formation of a Single Crystalline Hydrogen‐bonded Crosslinked Organic Framework and Its Boron Trifluoride Uptake and Catalysis
Authors: Li Fangzhou , Li Errui , Samanta Krishanu , Zheng Zhaoxi , Wu Lianqian , Chen Albert D. , Farha Omar K. , Staples Richard J. , Niu Jia , Schmidt‐Rohr Klaus , Ke Chenfeng 
Published: October 2023

AbstractBoron trifluoride (BF3) is a highly corrosive gas widely used in industry. Confining BF3 in porous materials ensures safe and convenient handling and prevents its degradation. Hence, it is highly desired to develop porous materials with high adsorption capacity, high stability, and resistance to BF3 corrosion. Herein, we designed and synthesized a Lewis basic single‐crystalline hydrogen‐bond crosslinked organic framework (HCOF‐50) for BF3 storage and its application in catalysis. Specifically, we introduced self‐complementary ortho‐alkoxy‐benzamide hydrogen‐bonding moieties to direct the formation of highly organized hydrogen‐bonded networks, which were subsequently photo‐crosslinked to generate HCOFs. The HCOF‐50 features Lewis basic thioether linkages and electron‐rich pore surfaces for BF3 uptake. As a result, HCOF‐50 shows a record‐high 14.2 mmol/g BF3 uptake capacity. The BF3 uptake in HCOF‐50 is reversible, leading to the slow release of BF3. We leveraged this property to reduce the undesirable chain transfer and termination in the cationic polymerization of vinyl ethers. Polymers with higher molecular weights and lower polydispersity were generated compared to those synthesized using BF3 ⋅ Et2O. The elucidation of the structure–property relationship, as provided by the single‐crystal X‐ray structures, combined with the high BF3 uptake capacity and controlled sorption, highlights the molecular understanding of framework‐guest interactions in addressing contemporary challenges.
Surface Sites and Ligation in Amine‐capped CdSe Nanocrystals**
Authors: Cao Weicheng , Yakimov Alexander , Qian Xudong , Li Jiongzhao , Peng Xiaogang , Kong Xueqian , Copéret Christophe 
Published: October 2023

AbstractConverting colloidal nanocrystals (NCs) into devices for various applications is facilitated by designing and controlling their surface properties. One key strategy for tailoring surface properties is thus to choose tailored surface ligands. In that context, amines have been universally used, with the goal to improve NCs synthesis, processing and performances. However, understanding the nature of surface sites in amine‐capped NCs remains challenging, due to the complex surface compositions as well as surface ligands dynamic. Here, we investigate both surface sites and amine ligation in CdSe NCs by combining advanced NMR spectroscopy and computational modelling. Notably, dynamic nuclear polarization (DNP) enhanced 113Cd and 77Se 1D NMR helps to identify both bulk and surface sites of NCs, while 113Cd 2D NMR spectroscopy enables to resolve amines terminated sites on both Se‐rich and nonpolar surfaces. In addition to directly bonding to surface sites, amines are shown to also interact through hydrogen‐bonding with absorbed water as revealed by 15N NMR, augmented with computations. The characterization methodology developed for this work provides unique molecular‐level insight into the surface sites of a range of amine‐capped CdSe NCs, and paves the way to identify structure‐function relationships and rational approaches towards colloidal NCs with tailored properties.
Artificial Peptide‐Protein Necrosomes Promote Cell Death
Authors: Guo Ruo‐Chen , Wang Ning , Wang Weishu , Zhang Zeyu , Luo Wendi , Wang Yushi , Du Haiqin , Xu Yifei , Li Gongyu , Yu Zhilin 
Published: October 2023

AbstractThe presence of disordered region or large interacting surface within proteins significantly challenges the development of targeted drugs, commonly known as the “undruggable” issue. Here, we report a heterogeneous peptide‐protein assembling strategy to selectively phosphorylate proteins, thereby activating the necroptotic signaling pathway and promoting cell necroptosis. Inspired by the structures of natural necrosomes formed by receptor interacting protein kinases (RIPK) 1 and 3, the kinase‐biomimetic peptides are rationally designed by incorporating natural or D‐amino acids, or connecting D‐amino acids in a retro‐inverso (DRI) manner, leading to one RIPK3‐biomimetic peptide PR3 and three RIPK1‐biomimetic peptides. Individual peptides undergo self‐assembly into nanofibrils, whereas mixing RIPK1‐biomimetic peptides with PR3 accelerates and enhances assembly of PR3. In particular, RIPK1‐biomimetic peptide DRI‐PR1 exhibits reliable binding affinity with protein RIPK3, resulting in specific cytotoxicity to colon cancer cells that overexpress RIPK3. Mechanistic studies reveal the increased phosphorylation of RIPK3 induced by RIPK1‐biomimetic peptides, elucidating the activation of the necroptotic signaling pathway responsible for cell death without an obvious increase in secretion of inflammatory cytokines. Our findings highlight the potential of peptide‐protein hybrid aggregation as a promising approach to address the “undruggable” issue and provide alternative strategies for overcoming cancer resistance in the future.
Impact of steaming and roasting heat‐treatment on physico‐chemical and functional properties of walnut kernel
Authors: Zhao Xiaoyan , Ren Xiangrui , Liu Hongkai , Zhang Xiaowei , Wang Meng , Hu Haifang 
Published: October 2023

AbstractIn this study, the effect of steaming and roasting treatment on the physicochemical and functional properties of walnut kernel at 95°C for different time (15, 20, and 30 min) was investigated. Steaming and roasting treatments significantly increased the enthalpy change for protein denaturation, in‐vitro digestibility, viscosity, storage modulus (G′) and loss modulus (G″) (p < 0.05), the order from high to low was steaming (7.11–8.69 J g−1; the gastric and intestinal digestion: 1.21%–17.83% and 1.51%–27.31%, respectively; 134.04–450.49 Pa s; 214.28–1047.14 and 61.72–196.09 Pa) > roasting (6.24–7.07 J g−1; the gastric and intestinal digestion: 1.26–15.42% and 1.21–22.37% a, respectively; 16.92–86.07 Pa s; 6.58–209.85 and 3.08–67.14) > untreated (4.53 J g−1; the gastric and intestinal digestion: 0.24%–4.18% and 1.00–7.58%, respectively; 17.06 Pa s; 5.78 and 1.79 Pa). All samples contained the essential amino acids, the amino acid score of samples by steaming was the highest. In addition, the protein of walnut kernel after heating treatment contained more α‐helix and random coil structures compared to the untreated sample, while β‐sheet and β‐turns structures decreased. Moreover, the thermal treatment could cause the changes of the water/oil holding capacity, foaming and emulsifying properties of walnut kernel flour. When there were differences between the results of steaming and roasting samples, it was concluded that the water played an important role in steaming. These results suggested that the thermal treatment as an effective approach could improve the physico‐chemical, structural and functional properties of walnut kernel and be potentially applied in the food processing.
Rapid Olefin Cyclopropanation Catalyzed by a Bioinspired Cobalt Complex
Authors: Tan Liming , Wu Keyang , Li Gang 
Published: October 2023

AbstractCyclopropanes are important structural motifs found in many natural products and are essential to the pharmaceutical and agrochemical industries. Here, we report a bioinspired cobalt catalyst that catalyzes the intermolecular cyclopropanation of various terminal olefins using ethyl diazoacetate (EDA) in high efficiency. This cobalt catalytic system is operationally simple under very mild conditions, enabling the synthesis of cyclopropane products with remarkable yields in short reaction time. Preliminary mechanistic studies suggest the presence of cobalt carbene radical species during the reaction.
A scoping review of empirical research on emergent intraverbal behavior
Authors: Conine Daniel E. , Frampton Sarah E. , Buote Kyrsten A. , Keller Chelsea E. 
Published: October 2023

AbstractFor decades, Skinner's analysis of verbal behavior has been applied in a variety of contexts. One critically important topic in this area is the extent to which some verbal operants may be learned indirectly, as a result of learning other verbal operants. This phenomenon is often referred to as emergent verbal behavior, and is critical to our understanding of how language is learned in a generative fashion across the lifespan. Emergent intraverbal repertoires are especially important because responses under some degree of intraverbal control may constitute a majority of responses in a fully formed verbal repertoire. Recent literature reviews suggest that there are many published studies on emergent intraverbals, but that this body of literature is highly heterogeneous. To provide an overview of this literature and map out the various tactics used across studies, we conducted a scoping review. We identified 99 total experiments on emergent intraverbals contained in 79 total articles. Findings are summarized in terms of populations and independent variables studied, procedural variations, and recommendations for future research.
Glutamine‐limited batch hybridoma growth and antibody production: Experiment and model
Authors: Dalili Mina , Sayles Gregory D. , Ollis David F. 
Published: October 2023

AbstractThe influence of glutamine (a major energy source) on both hybridoma growth and monoclonal antibody production was examined. A series of batch experiments were performed in T‐flasks containing initial glutamine levels ranging from 0.5 to 4.0 mM in RPMI 1640 with 20% v/v fetal calf serum. The maximum final cell concentration increased with initial glutamine levels in the range of 0.5–2 mM; further glutamine increases had little or no effect. Earlier studies in our laboratories demonstrated that serum component(s) strongly influence the maximum specific growth rate. Here, the present studies reveal also the stoichiometric limitation by glutamine in the later stages of growth when its concentration is drastically reduced. For 0.5 to 1.5 mM initial glutamine, complete substrate utilization coincided with the cessation of cell growth and the onset of the death phase. For initial glutamine concentrations higher than 2.0 mM, growth halted prior to glutamine exhaustion, presumably because serum or RPMI component(s) were exhausted. The specific antibody secretion rate was essentially non‐growth‐associated above a critical low glutamine concentration in both the growth and death phases. At or below this critical value, an apparent emergence of stoichiometnc or energy limitation resulted in a dramatic drop in the secretion rate to zero. A simple unstructured model was developed that simulates these trends well. All parameters were determined using only subsets of the data. Nevertheless, these parameter values provided simulations in good agreement with all the glutamine‐limited cultures.
Employing an underwater vehicle in education as a learning tool for Python programming
Authors: Rousouliotis Minas , Vasileiou Marios , Manos Nikolaos , Kavallieratou Ergina 
Published: October 2023

AbstractGetting students motivated and interested in their education can be challenging in any classroom setting, even more so in an online learning environment. In this spectrum, educational robotics (ER) has demonstrated numerous advantages in the educational environment, not only by facilitating teaching, but also enabling the cultivation of manyfold skills, including creativity, problem‐solving, and teamwork. Meanwhile, many methods have been developed with the aid of technology to improve the teaching process and boost students' ability to learn. Blended learning is one approach that integrates conventional classroom methods with digital resources in an effort to foster students' creativity. But how can blended learning be combined with robotics? The objective of this paper is to evaluate the impact of employing an underwater vehicle, called educational underwater vehicle (EDUV), in conjunction with a dedicated programming learning platform within the context of a programming course that is offered at the high school level. In this work, this platform is utilized by students in secondary education, and a survey was conducted prior and after using the underwater vehicle's platform based on two questionnaires. The survey included 112 Greek participants, 64 males and 48 females in the age range of 14–18 years old. The experimental results show an increase in their motivation and creativity. In other words, they are more engaged in the classroom and the lesson becomes more enjoyable. More specifically, the survey revealed that most participants are familiar with computers but have limited knowledge of robotics and programming. After training on the EDUV platform, participants showed a significant increase in correct responses for Python and Blockly environments, with an average of 50.7% in four programming‐related questions. The platform also reduced “do not know” replies, which means that the student's self‐esteem increased. The paired sample T‐test showed that the EDUV platform positively influenced participants' perceptions of robotics and motivated them to further their education. In this paper, the related work is discussed, and the architecture of the vehicle is analyzed, along with the integration with the online platform. In addition, the methodology performed is explained and divided into steps. Finally, the experimental results are discussed. Instructions, 3D models, and code can be found in the github repository https://github.com/MariosVasileiou/EDUV.
Group psychotherapy format guided self‐help for patients with FNSD awaiting inpatient multidisciplinary treatment: A pilot study
Authors: Boico Vasile , Humblestone Susan , Moutoussis Michael , Selai Caroline 
Published: October 2023

AbstractBackgroundWe describe our experience of developing Guided Self Help for patients with Functional Neurological Symptom Disorder as group therapy, delivered remotely, via Zoom.AimsThe aims of the current study are (a) to describe how the original Queen Square Guided Self‐Help (QGSH) was adapted for group therapy, and (b) to present a service evaluation of the first 8 months of running QGSH therapy groups remotely.MethodsWe carried out a review after 8 months, from 11 May 2021 until 31 January 2022.ResultsA total of 16 patients were treated in three groups. Assessing our outcomes in terms of Yalom's framework for group psychotherapy, we found that the group provided 10/11 of Yalom's therapeutic factors. Patient satisfaction was high.DiscussionWe outline for plans for further devaluation and development, including the development of a stand‐alone group.ConclusionThe group therapy format is a promising addition to our service.
A Review on Bioactive Compounds, Ethnomedicinal Importance and Pharmacological Activities of Talinum triangulare (Jacq.) Willd
Authors: Dinesh Anagha , Kumar Ajay 
Published: October 2023

AbstractTalinum triangulare (Jacq.) Willd. is a traditional leafy vegetable used by tribal communities for ethnomedicinal and ethnoculinary preparations. This article reviews the current knowledge of its multiple uses, including pharmacological activities and nutritional composition. The literature survey shows that it has been traditionally useful in the treatment of several diseases, such as anaemia, diabetes, measles, and ulcers and the preparation of various traditional foods. Analysis of the literature on its phytochemicals shows its richness in bioactive compounds. Further, research also shows that this plant has antidiabetic, antiobesity, antitumor, antiulcer, hepatoprotective, and neuroprotective activities besides anti‐inflammatory and antioxidant properties. Nutrient analysis of the plant reveals the presence of Ca, Zn, Fe, vitamins C and E, dietary fibre and protein in considerable quantities. The results of the pharmacological studies on the antidiabetic, antiulcer and anti‐anaemic activities provide support in favour of its ethnomedicinal uses. The presence of bioactive compounds and pharmacological activities show the usefulness of this plant as a functional food.
Phytochemical Analysis of Anacamptis coriophora Plant Cultivated Using Ex Vitro Symbiotic Propagation
Authors: Aytar Erdi Can , Harzli Ines , Özdener Kömpe Yasemin 
Published: October 2023

AbstractThis study aims to investigate the functional groups and phytochemical profile of Anacamptis coriophora seeds, tubers, and flowers. Symbiotic seedlings produced using the ex vitro method were transferred to their natural habitat and grown to analyze the functional groups and phytochemical profiles of tubers and flowers. The life cycles of the transferred seedlings were monitored, and tubers and flowers were harvested for analysis. ATR‐FTIR spectroscopy revealed the presence of functional groups such as polysaccharides, lignin, and proteins in both tubers and flowers. Differences in spectral frequencies between first‐year and second‐year tubers were observed. Fatty acid analysis identified 30 different compounds in seeds, flowers, and tubers, with linoleic acid being the most abundant (27 % in seeds, 33 % in tubers), and palmitic acid present in flowers (24 %). GC‐MS analysis of ethanol extracts from these components highlighted the presence of 32 compounds, including hydroxyacetic acid, hydrazide, cytidine (Z)‐7‐hexadecenal, 2,2‐dimethoxyethane, 2,5,6‐trimethyldecane, and butanamide, 4‐amino‐N‐hydroxy. A. coriophora‘s tubers, flowers, and seeds may contain active metabolites with therapeutic potential. These results are valuable for the commercial cultivation of the plant.
Inducin Triggers LC3‐Lipidation and ESCRT‐Mediated Lysosomal Membrane Repair
Authors: Corkery Dale , Ursu Andrei , Lucas Belén , Grigalunas Michael , Kriegler Simon , Oliva Rosario , Dec Robert , Koska Sandra , Pahl Axel , Sievers Sonja , Ziegler Slava , Winter Roland , Wu Yao‐Wen , Waldmann Herbert 
Published: October 2023

AbstractLipidation of the LC3 protein has frequently been employed as a marker of autophagy. However, LC3‐lipidation is also triggered by stimuli not related to canonical autophagy. Therefore, characterization of the driving parameters for LC3 lipidation is crucial to understanding the biological roles of LC3. We identified a pseudo‐natural product, termed Inducin, that increases LC3 lipidation independently of canonical autophagy, impairs lysosomal function and rapidly recruits Galectin 3 to lysosomes. Inducin treatment promotes Endosomal Sorting Complex Required for Transport (ESCRT)‐dependent membrane repair and transcription factor EB (TFEB)‐dependent lysosome biogenesis ultimately leading to cell death.
Amulet device implantation following incomplete left atrial appendage closure with Watchman legacy device
Authors: Yoon Sung‐Han , Elgendy Akram Y. , Dallan Luis Augusto Palma , Filby Steven J. 
Published: October 2023

AbstractPeri‐device leak after left atrial appendage closure (LAAC) is often treated with endovascular coils, plugs, or second occluders. This is the first study reporting the Amulet device used for peri‐device leak. An 80‐year‐old male with paroxysmal atrial fibrillation and recurrent falls with head trauma who underwent LAAC with a 24 mm Watchman 2.5 device 3 years ago at another institution was referred to our clinic for management of the peri‐device leak. Transesophageal echocardiogram showed persistent residual peri‐device leak with 5 mm width along the Coumadin ridge aspect of the device. Computed tomography (CT) also showed the peri‐device leak with width of 6 mm and complete opacification of left atrial appendage (LAA). Importantly, CT demonstrated that the Watchman 2.5 device was deployed at distal LAA, leaving the proximal part of LAA with length of 10 mm from ostium. Under general anesthesia, a 22 mm Amulet device was deployed successfully with complete sealing of LAA. Procedure planning is the key to minimize the risk of peri‐device leak or device‐related thrombosis. Careful assessment of LAA anatomy using multimodality images for peri‐device leak after LAAC helped optimal treatment strategy including second LAAC with different type of devices.