bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intelligent neuromarketing framework for consumers' preference prediction from electroencephalography signals and eye tracking
Authors: Mashrur Fazla Rabbi , Rahman Khandoker Mahmudur , Miya Mohammad Tohidul Islam , Vaidyanathan Ravi , Anwar Syed Ferhat , Sarker Farhana , Mamun Khondaker A. 
Published: October 2023

AbstractNeuromarketing uses brain‐computer interface technology to understand customer preferences in response to marketing stimuli. Every year, marketing professionals spend over $750 Billion (US dollars) on traditional marketing, which is usually behavioral and subjective, focusing on self‐reports acquired via questionnaires, focus groups, and depth interviews. Neuromarketing, on the other hand, promises to overcome such limitations. This work proposes a machine learning framework that incorporates multiple components (endorsement, offer, and slogan) in real advertisement to predict consumer preference from electroencephalography (EEG) signals. In addition, we also use eye‐tracking data to visualize consumer viewing patterns according to both advertisement type and preference. EEG signals are collected from 22 healthy volunteers while viewing the real ads as stimuli. After preprocessing the signals, three‐domain features are extracted (time, frequency, and time‐frequency). Then, using wrapper‐based approaches we choose best features which are later classified into strong and weak preferences using the support vector machine. The experimental results demonstrate the best performance using all the frontal channels with an accuracy of 96.97%, sensitivity of 96.30%, and specificity of 97.44%. Additionally, eye tracking data reveals that subjects substantially prefer an ad, when they first glance at the endorsement. In addition, people tend to blink their eyes less frequently while viewing ads with endorsements and strongly prefer these commercials too. Additionally, our work lays the door for deploying such a neuromarketing framework in a real‐world context by employing consumer‐grade EEG equipment. Therefore, it is evident that neuromarketing technology may assist brands and companies in accurately predicting future customer preferences.
Regulation of HeLa cell proliferation and apoptosis by bovine lactoferrin
Authors: Shu Xingfu , Su Jinxian , Zhao Yu , Liu Chun , Chen Yao , Ma Xiaomei , Wang Zifan , Bai Jialin , Zhang Haixia , Ma Zhongren 
Published: October 2023

AbstractCervical cancer is one of the foremost common cancers in women. Lactoferrin (LF) has many biological functions, such as antitumor. This study aimed to explore the regulatory effect of bovine lactoferrin (bLF) on the proliferation and apoptosis of cervical cancer HeLa cells and to clarify the potential mechanism of action of bLF against HeLa cells. This study used CCK‐8, Trypan blue staining, and colony formation assays to verify the effect of bLF on HeLa cell proliferation. Hoechst 33258 fluorescence staining, AO/EB staining, and western blotting were used to determine the effects of bLF on apoptosis and autophagy in HeLa cells. We discovered that bLF significantly reduced the proliferation of HeLa cells in a dose‐ and time‐dependent manner compared to the control group. Furthermore, bLF primarily induced apoptosis in HeLa cells by increasing the expression of the proapoptotic proteins p53, Bax, and Cleaved‐caspase‐3 and downregulating the expression of the antiapoptotic protein Bcl‐2. In addition, the present study also showed that bLF treatment significantly activated autophagy‐related proteins LC3B‐II and Beclin I and down regulated the autophagosome transporter protein p62, indicating that bLF treatment can induce autophagy in HeLa cells. After pretreatment with the autophagy inhibitor, 3‐MA, which markedly found that autophagy inhibition by 3‐MA reversed bLF‐induced apoptosis, indicating that bLF can induce apoptosis by activating intracellular autophagy in HeLa cells. In the present study, our results support the theory of bLF significantly inhibited the proliferation of Hela cells by promoting apoptosis and reinforcing autophagy. The study will play an important role in therapying cervical cancer.
Photocatalyzed Carbon−Carbon Bond Formation Between Glycerol and Electron‐Deficient Olefins
Authors: El‐Chami Kassem , Madsen Robert 
Published: October 2023

AbstractGlycerol is produced in large quantities as a co‐product in the manufacturing of biodiesel and has received relatively little attention as a substrate for carbon−carbon bond‐forming reactions. In this work, the photocatalyzed reaction between glycerol and electron‐deficient olefins is investigated to regioselectively insert an alkyl group at C2 in the triol. The results show that the transformation is highly dependent on the nature of the photocatalyst and the olefin. In the presence of an iridium catalyst and quinuclidine, glycerol can be coupled at C2 with sterically unhindered acrylates. In the presence of tetrabutylammonium decatungstate, glycerol can be reacted with fumaronitrile, N‐methyl maleimide, methyl acrylate and itaconic anhydride where the latter two substrates require the additional presence of potassium persulfate as an oxidant. These findings give rise to several new carbon−carbon bond‐forming reactions with glycerol that allow the triol to be converted into more elaborate structures.
Engineering a Cl‐‐Modulated Light‐Driven Na+ Pump
Authors: Xiao Lan , Yang Qifan , Tan Jingjing , Ma Baofu , Chen Deliang 
Published: October 2023

AbstractMicrobial Na+‐pumping rhodopsin (NaR) is a promising optogenetic tool due to its unique ability to transport Na+. Like most rhodopsin‐based tools, NaR is limited to light‐based control. In this study, our objective was to develop a novel mode of modulation for NaR beyond light control. By introducing a potential Cl− binding site near the putative Na+ release cavity, we engineered Nonlabens dokdonensis rhodopsin 2 (NdR2) to be modulated by Cl−, an essential chemical in organisms. The engineered NdR2 demonstrated an approximately two‐fold increase in Na+ pump activity in the presence of 100 mM Cl− compared to Cl‐‐free solution. Increasing Cl− concentration decreased the lifetimes of the M and O intermediates accordingly. The analysis of competitive ion uptake suggested the bound Cl− may increase the Na+ affinity and selectivity. This chemical modulation allows for more diverse and precise control over cellular processes, advancing the development of next‐generation optogenetic tools. Notably, our Cl‐‐modulated NdR2 establishes an innovative mechanism for linking Cl− to Na+‐related processes, with potential applications in optogenetic therapies for related diseases.
A role for dopamine in control of the hypoxic ventilatory response via D2 receptors in the zebrafish gill
Authors: Reed Maddison , Pan Wen , Musa Lina , Arlotta Stefania , Mennigen Jan A. , Jonz Michael G. 
Published: October 2023

AbstractDopamine is a neurotransmitter involved in oxygen sensing and control of reflex hyperventilation. In aquatic vertebrates, oxygen sensing occurs in the gills via chemoreceptive neuroepithelial cells (NECs), but a mechanism for dopamine in autonomic control of ventilation has not been defined. We used immunohistochemistry and confocal microscopy to map the distribution of tyrosine hydroxylase (TH), an enzyme necessary for dopamine synthesis, in the gills of zebrafish. TH was found in nerve fibers of the gill filaments and respiratory lamellae. We further identified dopamine active transporter (dat) and vesicular monoamine transporter (vmat2) expression in neurons of the gill filaments using transgenic lines. Moreover, TH‐ and dat‐positive nerve fibers innervated NECs. In chemical screening assays, domperidone, a D2 receptor antagonist, increased ventilation frequency in zebrafish larvae in a dose‐dependent manner. When larvae were confronted with acute hypoxia, the D2 agonist, quinpirole, abolished the hyperventilatory response. Quantitative polymerase chain reaction confirmed expression of drd2a and drd2b (genes encoding D2 receptors) in the gills, and their relative abundance decreased following acclimation to hypoxia for 48 h. We localized D2 receptor immunoreactivity to NECs in the efferent gill filament epithelium, and a novel cell type in the afferent filament epithelium. We provide evidence for the synthesis and storage of dopamine by sensory nerve terminals that innervate NECs. We further suggest that D2 receptors on presynaptic NECs provide a feedback mechanism that attenuates the chemoreceptor response to hypoxia. Our studies suggest that a fundamental, modulatory role for dopamine in oxygen sensing arose early in vertebrate evolution.
Spectral Heterogeneity of Phenol Blue in Protic Solvents Revealed by Ultrafast Nonradiative Decay Dynamics
Authors: Hidaka Tsubasa , Tanaka Taketomo , Murai Takayuki , Teramoto Takahiro , Nagasawa Yutaka 
Published: October 2023

AbstractPhenol blue (PhB) is a dye that exhibits positive solvatochromism which is also nonfluorescent due to its ultrashort excited state lifetime. We have conducted femtosecond time‐resolved transient absorption (TA) spectroscopic measurements of a PhB solution using two distinct excitation wavelengths, at shorter and longer wavelength sides of the visible absorption band. The results revealed that a long‐lived heterogeneity exists in protic solvents whereas, in aprotic solvents, the system rapidly returns to the original thermal equilibrium on the ultrafast timescale. Interestingly, the lifetime of the heterogeneity is longer for methanol (MeOH) compared to ethanol (EtOH) solution suggesting stronger interaction between PhB and MeOH. Additionally, since the excited state lifetime of PhB was still in the sub‐picosecond domain even in a polymer matrix, we suggest a nonradiative decay mechanism through high‐frequency vibrational modes without involving large structural reconfiguration such as twisting of the benzoquinone (BzQ) moiety.
New Principle for Simple Water Detection using Fluorescence Lifetime Triangulenium Probes
Authors: Jakobsen Rasmus K. , Laursen Bo W. 
Published: October 2023

AbstractThe fluorescence of phenol‐substituted ADOTA triangulenium fluorophores is quenched as function of the water content in acetonitrile. We investigate three phenol‐ADOTA derivatives in various solvent mixtures and conclude that water acts as a dynamic quencher by serving as a proton acceptor in a proton coupled electron transfer reaction with the excited ADOTA. Beyond the mechanistic insights into this quenching mechanism we demonstrate how these ADOTA dyes can probe the water content from a simple calibration free single‐point measurement of the fluorescence lifetime.
Climate‐Smart IPM: Key to U.S. Ag Resilience
Authors: Green Thomas 
Published: October 2023

AbstractFarmers, ranchers, and foresters are facing increasing challenges related to changing climate and weather, manifested in rising average temperatures, more frequent extreme rainfall events, extended drought, and heat waves. These changes are also impacting pest abundance and distribution, creating new pest problems and straining current pest management options and decision‐making. Key factors for U.S. agriculture and producer resilience in tackling new environmental and pest challenges lie in the ability of producers to apply effective, economical integrated pest management (IPM) approaches and in support for research and development of new and renewed IPM tactics and systems to meet 21st century needs. Earn 0.5 CEUs in Integrated Pest Management by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
Indole‐based aryl sulfides target the cell wall of Staphylococcus aureus without detectable resistance
Authors: Lavekar Aditya G. , Thakare Ritesh , Saima  , Equbal Danish , Chopra Sidharth , Sinha Arun K. 
Published: October 2023

AbstractSulfur‐containing classes of the scaffold “Arylthioindoles” have been evaluated for antibacterial activity; they demonstrated excellent potency against methicillin‐resistant Staphylococcus aureus (MRSA) as well as against vancomycin‐resistant strains and a panel of clinical isolates of resistant strains. In this study, we have elucidated the mechanism of action of lead compounds, wherein they target the cell wall of S. aureus. Further, S. aureus failed to develop resistance against two lead compounds tested in a serial passage experiment in the presence of the compounds over a period of 40 days. Both the compounds demonstrated comparable in vivo efficacy with vancomycin in a neutropenic mice thigh infection model. The results of these antibacterial activities emphasize the excellent potential of thioethers for developing novel antibiotics and may fill in as a target for the adjustment of accessible molecules to develop new powerful antibacterial agents with fewer side effects.
Exploring the combination of biochar‐amended soil and automated irrigation technology for water regulation and preservation in green infrastructure
Authors: Zhu Honghu , Huang Yuanxu , Song Haihong , Chen Jian , Han Songlei , Mazumder Tanwee , Garg Ankit 
Published: October 2023

AbstractBiochar is a carbon sink material with the potential to improve water retention in various soils. However, for the long‐term maintenance of green infrastructure, there is an additional need to regulate the water contents in the covers to maintain vegetation growth in semiarid conditions. In this study, biochar‐amended soil was combined with subsurface drip irrigation, and the water preservation characteristics of this treatment were investigated through a series of one‐dimensional soil column tests. To ascertain the best treatment method specific to semiarid climatic conditions, the test soil was amended with 0%, 1%, 3%, and 5% biochar. Automatic irrigation devices equipped with soil moisture sensors were used to control the subsurface water content with the aim of enhancing vegetation growth. Each soil column test lasted 150 h, during which the volumetric water contents and soil suction data were recorded. The experimental results reveal that the soil specimen amended with 3% biochar is the most water‐saving regardless of the time cost. Soil with a higher biochar content (e.g., 5%) consumes a more significant amount of water due to the enhancement of the water‐holding capacity. Based on the experimental results, it can be concluded that the appropriate ratio can be determined within 1%–3%, which can reduce not only the amount of irrigated/used water but also the time cost. Such technology can be explored for water content regulation in green infrastructure and the development of barriers for protecting the environment around deep underground waste containment.
Effects of stream drying, season, and distance to refuge on macroinvertebrate community structure in an arid intermittent stream basin
Authors: Hollien Kelsey D. , Gill Brian A. , Eppehimer Drew E. , Bogan Michael T. 
Published: October 2023

AbstractIntermittent streams are globally ubiquitous and represent a large percentage of stream networks. As climate change in many arid regions increases the frequency and intensity of drying disturbances, it is important to understand how aquatic biota will respond to such disturbances and how it would impact aquatic biodiversity. To address these topics, we sampled 10 stream reaches in the Sycamore Creek basin, an arid‐land stream in central Arizona (USA), with reach‐scale flow regimes ranging from perennial to highly intermittent. We sampled aquatic macroinvertebrates during 4 seasons to explore seasonal variability in community structure through flowing and drying phases. We also collected continuous flow data with remote data loggers to explore the impacts of intermittency and distance to perennial refuges on species richness, taxonomic composition and trait composition. Overall, richness was lower at intermittent reaches than perennial reaches, and richness values increased linearly as flow duration increased. We found no relationship between richness and distance to the nearest perennial refuge. Community assemblages differed significantly by season but were not distinct between perennial and intermittent reaches. Trait composition was also distinct between seasons and flow regimes, with traits such as a lack of diapause, longer life span and predatory feeding behaviours being indicators for perennial reaches. As climate change alters natural flow regimes, understanding the responses of macroinvertebrate community structure to drying disturbances in arid‐land streams can provide insight on aquatic community responses to climate change at larger scales.
Interleukin 23 receptor: Expression and regulation in immune cells
Authors: Mezghiche Ikram , Yahia‐Cherbal Hanane , Rogge Lars , Bianchi Elisabetta 
Published: October 2023

AbstractThe importance of IL‐23 and its specific receptor, IL‐23R, in the pathogenesis of several chronic inflammatory diseases has been established, but the underlying pathological mechanisms are not fully understood. This review focuses on IL‐23R expression and regulation in immune cells.
Is conditioned pain modulation (CPM) affected by negative emotional state?
Authors: Horn‐Hofmann Claudia , Jablonowski Lena , Madden Melanie , Kunz Miriam , Lautenbacher Stefan 
Published: October 2023

AbstractBackgroundConditioned pain modulation (CPM) is an experimental paradigm, which describes the inhibition of responses to a noxious or strong‐innocuous stimulus, the test stimulus (TS), by the additional application of a second noxious or strong‐innocuous stimulus, the conditioning stimulus (CS). As inadequate CPM efficiency has been assumed to be predisposing for clinical pain, the search for moderating factors explaining inter‐individual variations in CPM is ongoing. Psychological factors have received credits in this context. However, research concerning associations between CPM and trait factors relating to negative emotions has yielded disappointing results. Yet, the influence of anxious or fearful states on CPM has not attracted much interest despite ample evidence that negative affective states enhance pain. Our study aimed at investigating the effect of fear induction by symbolic threat on CPM.MethodsThirty‐seven healthy participants completed two experimental blocks: one presenting aversive pictures showing burn wounds (high‐threat block) and one presenting neutral pictures (low‐threat block). Both blocks contained a CPM paradigm with contact heat as TS and hot water as CS; subjective numerical ratings as well as contact‐heat evoked potentials (CHEPs) were assessed.ResultsWe detected an overall inhibitory CPM effect for CHEPs amplitudes but not for pain ratings. However, we found no evidence for a modulation of CPM by threat despite threat ratings indicating that our manipulation was successful.DiscussionThese results suggest that heat/thermal CPM is resistant to this specific type of symbolic threat induction and further research is necessary to examine whether it is resistant to fearful states in general.SignificanceThe attempt of modulating heat conditioned pain modulation (CPM) by emotional threat (fear/anxiety state) failed. Thus, heat CPM inhibition again appeared resistant to emotional influences. Pain‐related brain potentials proved to be more sensitive for CPM effects than subjective ratings.
A noninvasive multianalytical approach establishment for risk assessment and gastric cancer screening
Authors: Fan Xiao‐Han , Zhang Yang , Wang Pei , Song Qian‐Qian , Wang Mona , Mejias‐Luque Raquel , Li Zhe‐Xuan , Zhou Tong , Zhang Jing‐Ying , Liu Wei‐Dong , Zhang Lan‐Fu , Li Wen‐Qing , You Wei‐Cheng , Gerhard Markus , Jiao Yu‐Chen , Wang Xiao‐Bing , Pan Kai‐Feng 
Published: October 2023

AbstractEffective screening and early detection are critical to improve the prognosis of gastric cancer (GC). Our study aims to explore noninvasive multianalytical biomarkers and construct integrative models for preliminary risk assessment and GC detection. Whole genomewide methylation marker discovery was conducted with CpG tandems target amplification (CTTA) in cfDNA from large asymptomatic screening participants in a high‐risk area of GC. The methylation and mutation candidates were validated simultaneously using one plasma from patients at various gastric lesion stages by multiplex profiling with Mutation Capsule Plus (MCP). Helicobacter pylori specific antibodies were detected with a recomLine assay. Integrated models were constructed and validated by the combination of multianalytical biomarkers. A total of 146 and 120 novel methylation markers were found in CpG islands and promoter regions across the genome with CTTA. The methylation markers together with the candidate mutations were validated with MCP and used to establish a 133‐methylation‐marker panel for risk assessment of suspicious precancerous lesions and GC cases and a 49‐methylation‐marker panel as well as a 144‐amplicon‐mutation panel for GC detection. An integrated model comprising both methylation and specific antibody panels performed better for risk assessment than a traditional model (AUC, 0.83 and 0.63, P < .001). A second model for GC detection integrating methylation and mutation panels also outperformed the traditional model (AUC, 0.82 and 0.68, P = .005). Our study established methylation, mutation and H. pylori‐specific antibody panels and constructed two integrated models for risk assessment and GC screening. Our findings provide new insights for a more precise GC screening strategy in the future.
Spectacled Flying‐fox conflicts—tucker, totem, taunt and threat
Authors: Preece Noel 
Published: October 2023

AbstractFlying‐foxes worldwide have suffered population declines and some extinctions, and due to negative attitudes to bats, achieving population recovery is challenging. The Spectacled Flying‐fox of north‐east Australia, a species vital to the wet tropics region, experienced a population crash of over 75% in <15 years. Despite this decline, little action has been taken over the last two decades to help the species recover. The scientific evidence of the continuing population decline of the Spectacled Flying‐fox has been presented to multiple levels of government, but detrimental decisions have been made despite the evidence. Scientific evidence and arguments by themselves are clearly not sufficient to change attitudes. The approach and narrative have to change to persuade people that the species is important for the rainforests and other forests that people love. Better engagement, narrative and story‐telling are required. Bringing together communication specialists, social scientists and wildlife scientists are necessary to create narratives that people understand and accept, and that persuades them that the Spectacled Flying‐fox is worth protecting. Actions to reduce impacts on the human community are essential but need to be done in tandem with changing hearts and minds. Otherwise, the Spectacled Flying‐fox will continue its decline.