bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays
Authors: Adjaye James , Herwig Ralf , Herrmann Doris , Wruck Wasco , BenKahla Alia , Brink Thore C , Nowak Monika , Carnwath Joseph W , Hultschig Claus , Niemann Heiner , Lehrach Hans 
Published: November 2004

Abstract
Background
Cross-species gene-expression comparison is a powerful tool for the discovery of evolutionarily conserved mechanisms and pathways of expression control. The usefulness of cDNA microarrays in this context is that broad areas of homology are compared and hybridization probes are sufficiently large that small inter-species differences in nucleotide sequence would not affect the analytical results. This comparative genomics approach would allow a common set of genes within a specific developmental, metabolic, or disease-related gene pathway to be evaluated in experimental models of human diseases. The objective of this study was to investigate the feasibility and reproducibility of cross-species analysis employing a human cDNA microarray as probe.

Results
As a proof of principle, total RNA derived from human and bovine fetal brains was used as a source of labelled targets for hybridisation onto a human cDNA microarray composed of 349 characterised genes. Each gene was spotted 20 times representing 6,980 data points thus enabling highly reproducible spot quantification. Employing high stringency hybridisation and washing conditions, followed by data analysis, revealed slight differences in the expression levels and reproducibility of the signals between the two species. We also assigned each of the genes into three expression level categories- i.e. high, medium and low. The correlation co-efficient of cross hybridisation between the orthologous genes was 0.94. Verification of the array data by semi-quantitative RT-PCR using common primer sequences enabled co-amplification of both human and bovine transcripts. Finally, we were able to assign gene names to previously uncharacterised bovine ESTs.

Conclusions
Results of our study demonstrate the harnessing and utilisation power of comparative genomics and prove the feasibility of using human microarrays to facilitate the identification of co-expressed orthologous genes in common tissues derived from different species.
Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1receptor with neuronal and glial markers
Authors: Niles Lennard P , Armstrong Kristen J , Rincón Castro Lyda M , Dao Chung V , Sharma Rohita , McMillan Catherine R , Doering Laurie C , Kirkham David L 
Published: November 2004

Abstract
Background
In order to optimize the potential benefits of neural stem cell (NSC) transplantation for the treatment of neurodegenerative disorders, it is necessary to understand their biological characteristics. Although neurotrophin transduction strategies are promising, alternative approaches such as the modulation of intrinsic neurotrophin expression by NSCs, could also be beneficial. Therefore, utilizing the C17.2 neural stem cell line, we have examined the expression of selected neurotrophic factors under different in vitro conditions. In view of recent evidence suggesting a role for the pineal hormone melatonin in vertebrate development, it was also of interest to determine whether its G protein-coupled MT1 and MT2 receptors are expressed in NSCs.

Results
RT-PCR analysis revealed robust expression of glial cell-line derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in undifferentiated cells maintained for two days in culture. After one week, differentiating cells continued to exhibit high expression of BDNF and NGF, but GDNF expression was lower or absent, depending on the culture conditions utilized. Melatonin MT1 receptor mRNA was detected in NSCs maintained for two days in culture, but the MT2 receptor was not seen. An immature MT1 receptor of about 30 kDa was detected by western blotting in NSCs cultured for two days, whereas a mature receptor of about 40 – 45 kDa was present in cells maintained for longer periods. Immunocytochemical studies demonstrated that the MT1 receptor is expressed in both neural (β-tubulin III positive) and glial (GFAP positive) progenitor cells. An examination of the effects of melatonin on neurotrophin expression revealed that low physiological concentrations of this hormone caused a significant induction of GDNF mRNA expression in NSCs following treatment for 24 hours.

Conclusions
The phenotypic characteristics of C17.2 cells suggest that they are a heterogeneous population of NSCs including both neural and glial progenitors, as observed under the cell culture conditions used in this study. These NSCs have an intrinsic ability to express neurotrophic factors, with an apparent suppression of GDNF expression after several days in culture. The detection of melatonin receptors in neural stem/progenitor cells suggests involvement of this pleiotropic hormone in mammalian neurodevelopment. Moreover, the ability of melatonin to induce GDNF expression in C17.2 cells supports a functional role for the MT1 receptor expressed in these NSCs. In view of the potency of GDNF in promoting the survival of dopaminergic neurons, these novel findings have implications for the utilization of melatonin in neuroprotective strategies, especially in Parkinson's disease.
An evaluation of UV protection imparted by cotton fabrics dyed with natural colorants
Authors: Sarkar Ajoy K 
Published: November 2004

Abstract
Background
The ultraviolet properties of textiles dyed with synthetic dyes have been widely reported in literature. However, no study has investigated the ultraviolet properties of natural fabrics dyed with natural colorants. This study reports the Ultraviolet Protection Factor (UPF) of cotton fabrics dyed with colorants of plant and insect origins.

Methods
Three cotton fabrics were dyed with three natural colorants. Fabrics were characterized with respect to fabric construction, weight, thickness and thread count. Influence of fabric characteristics on Ultraviolet Protection Factor was studied. Role of colorant concentration on the ultraviolet protection factor was examined via color strength analysis.

Results
A positive correlation was observed between the weight of the fabric and their UPF values. Similarly, thicker fabrics offered more protection from ultraviolet rays. Thread count appears to negatively correlate with UPF. Dyeing with natural colorants dramatically increased the protective abilities of all three fabric constructions. Additionally, within the same fabric type UPF values increased with higher depths of shade.

Conclusion
Dyeing cotton fabrics with natural colorants increases the ultraviolet protective abilities of the fabrics and can be considered as an effective protection against ultraviolet rays. The UPF is further enhanced with colorant of dark hues and with high concentration of the colorant in the fabric.
In vivogene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery
Authors: Chapel Alain , Deas Olivier , Bensidhoum Morad , François Sabine , Mouiseddine Moubarak , Poncet Pascal , Dürrbach Antoine , Aigueperse Jocelyne , Gourmelon Patrick , Gorin Norbert C , Hirsch François , Thierry Dominique 
Published: November 2004

Abstract
Background
Targeted gene transfection remains a crucial issue to permit the real development of genetic therapy. As such, in vivo targeted transfection of specific subsets of hematopoietic stem cells might help to sustain hematopoietic recovery from bone marrow aplasia by providing local production of growth factors.

Methods
Balb/C mice were injected intravenously, with an anti-mouse c-kit (CD117) monoclonal antibody chemically coupled to a human IL-3 gene-containing plasmid DNA. Mice were sacrificed for tissue analyses at various days after injection of the conjugates.

Results
By ELISA, the production of human IL-3 was evidenced in the sera of animals 5 days after treatment. Cytofluorometric analysis after in vivo transfection of a reporter gene eGFP demonstrated transfection of CD117+/Sca1+ hematopoietic immature cells. By PCR analysis of genomic DNA and RNA using primer specific pIL3 sequences, presence and expression of the human IL-3-transgene were detected in the bone marrow up to 10 days in transfected mice but not in control animals.

Conclusions
These data clearly indicate that antibody-mediated endocytosis gene transfer allows the expression of the IL-3 transgene into hematopoietic immature cells, in vivo. While availability of marketed recombinant growth factors is restricted, this targeting strategy should permit delivery of therapeutic genes to tissues of interest through systemic delivery. In particular, the ability to specifically target growth factor expression into repopulating hematopoietic stem cells may create new opportunities for the treatment of primary or radiation-induced marrow failures.
Human cytomegalovirus inhibits the migration of immature dendritic cells by down-regulating cell-surface CCR1 and CCR5
Authors: Varani Stefania , Frascaroli Giada , Homman-Loudiyi Mohammed , Feld Sari , Landini Maria Paola , Söderberg-Nauclér Cecilia 
Published: November 2004

Abstract
Dendritic cells (DC) play a key role in the host immune response to infections. Human cytomegalovirus (HCMV) infection can inhibit the maturation of DC and impair their ability to stimulate T cell proliferation and cytotoxicity. In this study, we assessed the effects of HCMV infection on the migratory behavior of human DC. The HCMV strain TB40/E inhibited the migration of immature monocyte-derived DC in response to inflammatory chemokines by 95% 1 day after infection. This inhibition was mediated by early viral replicative events, which significantly reduced the cell-surface expression of CC chemokine receptor 1 (CCR1) and CCR5 by receptor internalization. HCMV infection also induced secretion of the inflammatory chemokines CC chemokine ligand 3 (CCL3)/macrophage inflammatory protein-1α (MIP-1α), CCL4/MIP-1β, and CCL5/regulated on activation, normal T expressed and secreted (RANTES). Neutralizing antibodies for these chemokines reduced the effects of HCMV on chemokine receptor expression and on DC migration by ∼60%. Interestingly, the surface expression of the lymphoid chemokine receptor CCR7 was not up-regulated after HCMV infection on immature DC, and immature-infected DC did not migrate in response to CCL19/MIP-3β. These findings suggest that blocking the migratory ability of DC may be a potent mechanism used by HCMV to paralyze the early immune response of the host.
Higher Mortality After Allogeneic Peripheral-Blood Transplantation Compared With Bone Marrow in Children and Adolescents: The Histocompatibility and Alternate Stem Cell Source Working Committee of the International Bone Marrow Transplant Registry
Authors: Eapen Mary , Horowitz Mary M. , Klein John P. , Champlin Richard E. , Loberiza Fausto R. , Ringdén Olle , Wagner John E. 
Published: November 2004

Purpose Peripheral-blood stem cells (PBSC) may be used as an alternative to bone marrow (BM) for allogeneic transplantation. Despite lack of data on PBSC transplantation in children, there has been a change in clinical practice, with increasing numbers of children receiving PBSC allografts. Patients and Methods We compared the results of 143 PBSC and 630 BM transplants from human leukocyte antigen–identical sibling donors in children aged 8 to 20 years with acute leukemia. PBSC transplant recipients were older, and were more likely to have advanced leukemia, receive growth factors post-transplantation, and have undergone transplantation more recently. Risks of acute and chronic graft-versus-host disease (GVHD), treatment-related mortality, relapse, treatment failure (relapse or death), and overall mortality were compared using Cox proportional hazards regression to adjust for potentially confounding factors. Results Hematopoietic recovery was faster after PBSC transplantation. Risks of grade 2 to 4 acute GVHD were similar, but chronic GVHD risk was higher after PBSC transplantation (relative risk [RR], 1.85; 95% CI, 1.28 to 2.66; P = .001). In contrast to reports in adults, treatment-related mortality (RR, 1.89; 95% CI, 1.28 to 2.80; P = .001), treatment failure (RR, 1.31; 95% CI, 1.03 to 1.68; P = .03), and mortality (RR, 1.38; 95% CI, 1.07 to 1.79; P = .01) were higher after PBSC transplantation. Risks of relapse were similar. Conclusion These data suggest poorer outcomes after PBSC compared with BM transplantation in children after adjusting for relevant risk factors. Given the trend toward increased use of PBSC allografts in children, prospective clinical trials are required to determine their appropriate role in this group of patients.
Dynamic stability of viscoelastic columns loaded by a follower force
Authors: Darabseh T T , Genin J 
Published: November 2004

This paper analyses the motion of a linear viscoelastic column using the dynamic approach. The viscoelastic material is mathematically represented by the four-element model. Using this model as a recursion equation, the elastic, one-, two-, three- and four-element models are solved and their results compared. The stability analyses of these systems are investigated by studying the eigenvalues of the characteristic equations for the solution to the boundary value problems. The stability analyses determined the smallest value of the follower load beyond which the system, under a suitable disturbance, will perform oscillations with increasing amplitudes. The dynamic instability is found to occur in the form of flutter. The effect of damping on the critical follower load is also examined. Three damping parameters are considered that affect the results. They are the damping coefficients related to a Maxwell unit, to a Kelvin unit and one appropriate to a combination of the two. The destabilization effects of viscosity are discussed.
Performance analysis of air-lift pump design
Authors: Awari G K , Ardhapurkar P M , Wakde D G , Bhuyar L B 
Published: November 2004

The air-lift pump is a well-established method for vertical transport of liquids and solid-liquid mixtures. The objectives of the present study are to evaluate the performance of a pump under predetermined operating conditions and to optimize the related parameters for the use of the pump for two-phase mixtures of air and water when a compressor is readily available. This paper is focused on the general mathematical functions applicable to air-lift pump installations. The effect of various design parameters on the performance of air-lift pump is presented. A user-friendly computer program is developed on the basis of the optimization of influencing variables. This program will be useful for the design and installation of an air-lift pump handling air-water mixtures. A comparative study of an air-lift pump with a centrifugal pump handling two-phase mixtures is also discussed in the paper.
Long-Acting Injectable Risperidone
Authors: Ehret Megan J , Fuller Matthew A 
Published: November 2004

OBJECTIVE To review the pharmacology, pharmacokinetics, clinical efficacy, and safety profile of long-acting (LA) risperidone for the treatment of schizophrenia. DATA SOURCES Information was selected from PubMed (1965–July 2004). Applicable scientific posters were also used. STUDY SELECTION AND DATA EXTRACTION All published information on risperidone LA was considered. Material providing a comprehensive description was considered. DATA SYNTHESIS Risperidone LA is the first long-acting, injectable atypical antipsychotic. It is dosed at 25–50 mg every 2 weeks. Adverse effects are similar to those seen with oral risperidone. A short-term study showed that risperidone LA is better than placebo in reducing the signs and symptoms of schizophrenia, and a long-term trial showed that stable schizophrenic patients can be switched from either oral or other injectable antipsychotic medications to risperidone LA. CONCLUSIONS Risperidone LA is efficacious and safe in the treatment of schizophrenia.
First Patient-Centered Drug Information Service in Germany—A Descriptive Study
Authors: Maywald Ulf , Schindler Christoph , Krappweis Jutta , Kirch Wilhelm 
Published: November 2004

BACKGROUND The majority of patients have unmet drug information needs, and patients’ knowledge of their own treatment is often poor. This is a major obstacle to a more patient-centered healthcare system. OBJECTIVE To explore patterns of patients’ unmet drug information needs and outcomes of counseling patients by a drug and therapy information center (DTIC). METHODS We established the first German DTIC for patients. In this descriptive study, data obtained on callers within 24 months were analyzed. Questionnaires for gathering information about callers’ characteristics and the outcome of the advice were sent to all patients using the service after consultation. Data on all inquiries and evaluation sheets were documented and analyzed by a standardized database. RESULTS During 24 months, 2049 telephone calls were recorded. Patients’ unmet information needs were mainly related to adverse drug reactions/drug interactions (31.0%) and therapy information (27.2%). In 81.0% of the cases, patients’ uncertainties regarding their prescribed medications were reduced, 37.9% discussed the advice with their physician, and 18.3% reported a reduction of physician visits as a result of our advice. The patient–physician relationship remained mainly (70.6%) unaffected after our intervention. CONCLUSIONS The DTIC is useful as a source of medical expert advice. The service can help to reduce medication problems based on inadequate information and therefore avoid unnecessary healthcare utilization. With the knowledge provided, patients can participate in decisions affecting their own health. This procedure encourages greater confidence in the merits of drug therapy.
Does the cue help? Children's understanding of multiplicative concepts in different problem contexts
Authors: Squire Sarah , Davies Charlotte , Bryant Peter 
Published: November 2004

Background: Understanding arithmetical principles is a key part of a conceptual understanding of mathematics. However, very little attention has been paid to children's understanding of multiplicative, as compared to additive, principles.Aims: This study investigated(a) children's ability to use commutative and distributive cues to solve multiplication problems, (b) whether their ability to use these cues depends on the problem context, and(c) whether separate mechanisms might underlie children's understanding of commutativity and distributivity.Sample: Twenty‐seven 9‐year‐olds (Year 5) and thirty‐two 10‐year‐olds (Year 6).Methods: Forty‐eight multiplication problems (with a multiple‐choice response format) were presented to children. There were four types of problem: Commutative, Distributive, Combined commutative‐distributive(all preceded by a cue) and No cue problems. Each type of problem was presented in three different contexts: Isomorphism of measures, Area, and Cartesian product.Results: Children demonstrated a good understanding of commutativity but a very poor understanding of distributivity. A common mistake in the distributive problems was to select the number that was one more, or one less, than the answer in the cue. Children's understanding of distributivity (butnot commutativity) seemed to depend on the problem context. Factor analysis suggested that separate factors underlie the ability to solve commutative and distributive problems.Conclusions: Nine‐ and 10‐year‐olds understand commutativity, but are unable to use the distributive principle in multiplication. Their errors suggest that they may confuse some of the principles of multiplication with those of addition. When children do begin to understand the principle of distributivity, they most easily apply it in the context of Isomorphism of measures multiplication problems. The implications for mathematical education are discussed.
Consistency in reasoning test scores over time
Authors: Strand Steve 
Published: November 2004

Background: UK schools have a long history of using reasoning tests, most frequently of Verbal Reasoning (VR), Non‐Verbal Reasoning (NVR), and to a lesser extent Quantitative Reasoning (QR). Results are used for identifying students' learning needs, for grouping students, for identifying underachievement, and for providing indicators of future academic performance. Despite this widespread use there are little empirical data on the long‐term consistency of VR, QR and NVR as discrete abilities.Aims: To evaluate and compare the consistency of VR, QR and NVR scores over a 3‐year period, and to explore the influence of the secondary school on pupils' progress in the tests.Sample: Data were collected on a longitudinal sample of over 10,000 pupils who completed the Cognitive Abilities Test Second Edition in year 6 (age 10+) and year 9 (age 13+), and GCSE public examinations in year 11 (age 15+).Methods: Correlation coefficients and change scores for individual pupils are calculated. Multilevel modelling is used to determine school effects on reasoning scores and GCSE public examination results.Results: The results reveal high correlations in scores over time, ranging from .87 for VR to .76 for NVR, but also show around one‐sixth of pupils on the VR test and one‐fifth of pupils on the QR and NVR tests change their scores by 10 or more standard score points. Schools account for only a small part of the total variation in reasoning score, although they account for a much greater proportion of the variation in measures of attainment such as GCSE. School effects on pupils' progress in the reasoning tests between age 10 and age 13 are relatively modest.Conclusions: Reasoning tests make excellent baseline assessments for secondary schools. Some practical and policy implications for schools are discussed.
Transmission Ratio Distortion in Offspring of Mouse Heterozygous Carriers of a (7.18) Robertsonian Translocation
Authors: Underkoffler Lara A , Mitchell Laura E , Abdulali Zaki S , Collins Joelle N , Oakey Rebecca J 
Published: November 2004

AbstractTransmission ratio distortion (TRD) is defined as a significant departure from expected Mendelian ratios of inheritance of an allele or chromosome. TRD is observed among specific regions of the mouse and human genome and is frequently associated with chromosome rearrangements such as Robertsonian (Rb) chromosomes. We intercrossed mice heterozygous for a (7.18) Rb translocation and genotyped chromosomes 7 and 18 in 1812 individuals, 47% of which were informative for chromosome segregation. We substantiated previous findings that females were less likely than expected to transmit the Rb chromosome to their offspring. Surprisingly, however, we report that heterozygous males transmitted the Rb translocation chromosome significantly more frequently than the acrocentrics. The transmission of the Rb chromosome was not significantly influenced by either the sex of the Rb grandparent or the strain of the Rb.