bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large‐scale physical facility and experimental dataset for the validation of urban drainage models
Authors: Sañudo E. , Cea L. , Puertas J. , Naves J. , Anta J. 
Published: January 2024

AbstractNumerical models are currently the main tool used to simulate the effects of urban flooding. The validation of these models requires thorough and accurate observed data in order to test their performance. The current study presents a series of laboratory experiments in a large‐scale urban drainage physical facility of approximately 100 m2 that includes roofs, streets, inlets, manholes and sewers. The facility is equipped with a rainfall simulator as well as a surface runoff and pipe inflows generators. The experiments were divided in two sets. In Set 1 the surface runoff was generated exclusively by the rainfall input, while in Set 2 the rainfall simulator was used in combination with the runoff generators. In all the tests the water discharge was measured at points on the inlets, roofs, and outfall. The water depth at different locations of the facility was also measured. The experimental tests were replicated numerically using the urban drainage model Iber‐SWMM. Experimental results show that, even in a relatively small catchment the peaks in the hydrographs generated at each element of the facility during intermittent rainfalls are significantly attenuated at the catchment outlet. The agreement between the experimental and numerical results show that there are some differences in the hydrographs generated at each element, but that these differences compensate each other and disappear at the outfall. The results generated provide the research community with a thorough and high‐resolution dataset obtained under controlled laboratory conditions in a large‐scale urban drainage facility, something which has not previously been available.
Efficacy of dupilumab plus topical corticosteroids in children with atopic dermatitis: A meta‐analysis of randomized controlled trials
Authors: Wang Li , Wang Si‐Ning , Zhang Ruil‐Li 
Published: January 2024

AbstractIntroductionChildren with atopic dermatitis (AD) bear a significant burden of illness that adversely affects their quality of life.ObjectiveTo determine the efficacy of dupilumab and topical corticosteroids for the treatment of pediatric AD.MethodsA comprehensive literature search was conducted using three prominent databases: Web of Science, PubMed, and Embase. Using a fixed‐effects or random‐effects model, the standard mean difference or risk ratios with 95% confidence intervals were calculated, and the trial protocol was listed as CRD42023408546.ResultsA total of 3 studies were included, and 896 participants met the inclusion criteria. The combined estimate showed that dupilumab plus topical corticosteroids had numerically greater efficacy in terms of Eczema Area and Severity Index (EASI)‐50, EASI‐75, EASI‐90, and Investigator Global Assessment (IGA) score of 0 or 1. Children who received topical corticosteroids and dupilumab achieved significantly higher Children's Dermatological Life Quality Index scores compared to those who received placebo. The number of individuals who achieved IGA 0/1 increased with the use of dupilumab and topical corticosteroids.ConclusionsDupilumab and topical corticosteroids can be used to treat symptoms in children with AD. However, given the substantial variation in treatment outcomes among studies, the findings should be interpreted with caution.
Sirtuin 3 is required for the dexmedetomidine‐mediated alleviation of inflammation and oxidative stress in nephritis
Authors: Lu Kai , Li Xinlong , Wu Jie 
Published: January 2024

AbstractIntroductionAlthough sirtuin 3 (SIRT3) is known to be involved in dexmedetomidine (DEX)‐mediated alleviation of renal ischemia and reperfusion injury, the influence of the association between DEX and SIRT3 on nephritis development remains unclear. In this study, the role of SIRT3 in DEX‐mediated amelioration of inflammation and oxidative stress in nephritis as well as the possible underlying mechanism were explored in vivo and in vitro.MethodsAn animal model of glomerulonephritis was generated by injecting mice with interferon‐alpha (IFNα)‐expressing adenoviruses, and periodic acid–Schiff staining was then used to reveal pathogenicity‐related changes in the renal tissue. Additionally, human embryonic kidney cells (HEK293) and renal mesangial cells (RMCs) were treated with IFNα to establish cell models of inflammation in vitro.ResultsDEX administration alleviated glomerulonephritis in the animal model and upregulated SIRT3 expression in the renal tissue. SIRT3 knockdown inhibited the renoprotective effects of DEX against nephritis. IFNα induced inflammation, oxidative stress, and apoptosis in the RMCs and HEK293 cells and reduced their growth, as evidenced by the evaluation of cytokine levels (enzyme‐linked immunosorbent assay), reactive oxygen species generation, catalase and superoxide dismutase activities, nuclear factor‐erythroid factor 2‐related factor 2/heme oxygenase‐1 signal transduction, apoptotic cell proportion, and cell viability. In addition to promoting SIRT3 expression, DEX inhibited IFNα‐induced inflammation, oxidative stress, and apoptosis in these cells and promoted their viability. SIRT3 knockdown partially reversed the beneficial effects of DEX on RMCs and HEK293 cells.ConclusionsOur results suggest that DEX exhibits renoprotective activity during nephritis progression, protecting renal cells against inflammatory injury by promoting SIRT3 expression.
An integrative model with HLA‐DR, CD64, and PD‐1 for the diagnostic and prognostic evaluation of sepsis
Authors: Chen Guosheng , Chong Huimin , Zhang Peng , Wen Dalin , Du Juan , Gao Chu , Zeng Shi , Zeng Ling , Deng Jin , Zhang Kejun , Zhang Anqiang 
Published: January 2024

AbstractBackgroundSepsis is a life‐threatening organ dysfunction caused by a dysregulated host response to infection and progressive immunosuppression with high mortality. HLA‐DR, CD64, and PD‐1 were assumed to be useful biomarkers for sepsis prediction. However, the ability of a combination of these biomarkers has not been clarified.MethodsAn observational case‐control study was conducted that included 30 sepsis patients, 30 critically ill patients without sepsis admitted to the intensive care unit (ICU), and 32 healthy individuals. The levels of HLA‐DR, CD64, and PD‐1 expression in peripheral blood immune cells and subsets was assayed on Days 1, 3, and 5, and the clinical information of patients was collected. We compared these biomarkers between groups and evaluated the predictive validity of single and combined biomarkers on sepsis mortality.ResultsThe results indicate that PD‐1 expression on CD4−CD8−T (PD‐1+CD4−CD8−T) (19.19% ± 10.78% vs. 9.88% ± 1.79%, p = .004) cells and neutrophil CD64 index (nCD64 index) (9.15 ± 5.46 vs. 5.33 ± 2.34, p = .001) of sepsis patients were significantly increased, and HLA‐DR expression on monocytes (mHLA‐DR+) was significantly reduced (13.26% ± 8.06% vs. 30.17% ± 21.42%, p = 2.54 × 10−4) compared with nonsepsis critically ill patients on the first day. Importantly, the expression of PD‐1+CD4−CD8−T (OR = 0.622, 95% CI = 0.423–0.916, p = .016) and mHLA‐DR+ (OR = 1.146, 95% CI = 1.014–1.295, p = .029) were significantly associated with sepsis mortality. For sepsis diagnosis, the mHLA‐DR+, PD‐1+CD4−CD8−T, and nCD64 index showed the moderate individual performance, and combinations of the three biomarkers achieved greater diagnostic value (AUC = 0.899, 95% CI = 0.792–0.962). When adding PCT into the combined model, the AUC increased to 0.936 (95% CI = 0.840–0.983). For sepsis mortality, combinations of PD‐1+CD4−CD8−T and mHLA‐DR+, have a good ability to predict the prognosis of sepsis patients, with an AUC = 0.921 (95% CI = 0.762–0.987).ConclusionThese findings indicate that the combinations of HLA‐DR, CD64, and PD‐1 outperformed each of the single indicator in diagnosis and predicting prognosis of sepsis.
Impacts of probiotics on the efficacies of immune checkpoint inhibitors with or without chemotherapy for patients with advanced non‐small‐cell lung cancer
Authors: Morita Ayako , Ichihara Eiki , Inoue Koji , Fujiwara Keiichi , Yokoyama Toshihide , Harada Daijiro , Ando Chihiro , Kano Hirohisa , Oda Naohiro , Tamura Tomoki , Ochi Nobuaki , Kawai Haruyuki , Inoue Masaaki , Hara Naofumi , Fujimoto Nobukazu , Ichikawa Hirohisa , Oze Isao , Hotta Katsuyuki , Maeda Yoshinobu , Kiura Katsuyuki 
Published: January 2024

AbstractThe relationships between the therapeutic effects of immune checkpoint inhibitors (ICIs) and the intestinal flora have attracted increasing attention. However, the effects of oral probiotics on the efficacies of ICIs used to treat non‐small‐cell lung cancer (NSCLC) remain unclear. We investigated the effects of probiotics on the efficacies of ICIs in patients treated with and without chemotherapy. We investigated patients with advanced NSCLC on ICI monotherapy or combination ICI and chemotherapy using the Okayama Lung Cancer Study Group Immunotherapy Database (OLCSG‐ID) and the Okayama Lung Cancer Study Group Immunochemotherapy Database (OLCSG‐ICD). In total, 927 patients (482 on ICI monotherapy, 445 on an ICI + chemotherapy) were enrolled. Most were male, of good performance status, smokers, and without epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK) mutations. Probiotics were administered to 19% of patients on ICI monotherapies and 17% of those on ICIs + chemotherapy. Of the former patients, progression‐free survival (PFS) and overall survival (OS) were significantly better in the probiotics group (PFS 7.9 vs. 2.9 months, hazard ratio [HR] 0.54, p < .001; OS not attained vs. 13.1 months, HR 0.45, p < .001). Among patients receiving ICI and chemotherapy, there were no significant differences in PFS between those on probiotics and not but OS was significantly better in the probiotics group (PFS 8.8 vs. 8.6 months, HR 0.89, p = .43; OS not attained vs. 22.6 months, HR 0.61, p = .03). Patients on probiotics experienced better outcomes following ICI treatment.
New mode of action of curcumin on prostate cancer cells: Modulation of endoplasmic reticulum‐associated degradation mechanism and estrogenic signaling
Authors: Erzurumlu Yalcin , Dogan Hatice Kubra , Catakli Deniz 
Published: January 2024

AbstractProstate cancer is leading to cancer‐related mortality in numerous men each year worldwide. While there are several treatment options, acquired drug resistance mostly limits the success of treatments. Therefore, there is a need for the development of innovative treatments. Curcumin is one of the bioactive polyphenolic ingredients identified in turmeric and has numerous biological activities, such as anti‐inflammatory and anticancer. In the present study, we investigated the effect of curcumin on the ER‐associated degradation (ERAD) and estrogenic signaling in prostate cancer cells. The antiproliferative effect of curcumin on human androgen‐dependent prostate cancer cell lines LNCaP and VCaP was estimated by WST‐1 assay. Morphological alterations were investigated with an inverted microscope. We investigated the effect of curcumin on ERAD and estrogen signaling proteins by immunoblotting assay. To evaluate the impact of curcumin on endoplasmic reticulum (ER) protein quality‐related, the expression level of 32 genes was analyzed by quantitative reverse transcription polymerase chain reaction. The nuclear translocation of estrogen receptor was examined by nuclear fractionation and immunofluorescence microscopy. We found that curcumin effectively reduced the proliferation rates of LNCaP and VCaP cells. ERAD proteins; Hrd1, gp78, p97/VCP, Ufd1 and Npl4 were strongly induced by curcumin. Also, the steady‐state level of polyubiquitin was increased in a dose‐dependent manner in both cell lines. Curcumin administration remarkably decreased the protein levels of estrogen receptor‐alfa (Erα), whereas estrogen receptor‐beta unaffected. Additionally, curcumin strongly restricted the nuclear translocation of Erα. Present data suggest that curcumin may be effectively used in therapeutic approaches associated with the targeting ER protein quality control mechanism and modulation of estrogen signaling in prostate cancer.
Exploring the Tl 2H 2 potential energy surface: A comparative analysis with group 13 systems and experiment
Authors: Tang Carson L. , Heide Alexander G. , Heide Alexandra D. , Douberly Gary E. , Turney Justin M. , Schaefer Henry F. 
Published: January 2024

AbstractThallium chemistry is experiencing unprecedented importance. Therefore, it is valuable to characterize some of the simplest thallium compounds. Stationary points along the singlet and triplet TlH potential energy surface have been characterized. Stationary point geometries were optimized with the CCSD(T)/aug‐cc‐pwCVQZ‐PP method. Harmonic vibrational frequencies were computed at the same level of theory while anharmonic vibrational frequencies were computed at the CCSD(T)/aug‐cc‐pwCVTZ‐PP level of theory. Final energetics were obtained with the CCSDT(Q) method. Basis sets up to augmented quintuple‐zeta cardinality (aug‐cc‐pwCV5Z‐PP) were employed to obtain energetics in order to extrapolate to the complete basis set limits using the focal point approach. Zero‐point vibrational energy corrections were appended to the extrapolated energies in order to determine relative energies at 0 K. It was found that the planar dibridged isomer lies lowest in energy while the linear structure lies highest in energy. The results were compared to other group 13 MH (M = B, Al, Ga, In, and Tl) theoretical studies and some interesting variations are found. With respect to experiment, incompatibilities exist.
Transgender and gender diverse individuals' perspectives on discussions of fetal sex chromosomes in obstetrics care
Authors: Tyrie Dana , Oliva Alejandra , Llorin Hannah , Zayhowski Kimberly 
Published: January 2024

AbstractIn the past decade, prenatal cell‐free DNA screening (cfDNA) has become ubiquitous as a screening tool for fetal aneuploidy and sex chromosomes. Healthcare provider (HCP) discussions and public perceptions of sex and gender uniquely impact transgender and gender diverse (TGD) individuals, and existing cfDNA guidelines lack recommendations regarding how to discuss sex and gender prenatally. The aim of this exploratory qualitative study was to examine TGD individuals' opinions regarding fetal sex chromosome disclosure sessions. Twelve semi‐structured virtual interviews were conducted with TGD individuals regarding their perspectives on the discussion of fetal sex chromosomes by HCPs within the prenatal setting. Interviews were coded and analyzed using a reflexive thematic approach, generating four major themes: (1) Current practices in prenatal care exclude gender diverse people; (2) HCPs' responsibility to de‐gender discussions of sex chromosomes in prenatal care; (3) HCPs' responsibility to acknowledge gender diversity; and (4) HCPs' influence on societal perceptions of sex and gender. More guidance is needed from professional societies regarding best practices for HCP discussions of sex chromosomes, sex, and gender. Participants recommended HCPs educate patients about sex chromosomes and their relevance to health while avoiding the conflation of sex and gender terms. Additionally, there is an acute need for trans‐inclusive prenatal healthcare. Ultimately, HCPs' and organizations are in a prime position to deconstruct rigid gender binaries and promote societal inclusion of TGD people.
Neuropsychological stability in classical galactosemia: A pilot study in 10 adult patients
Authors: Hermans Merel E. , Geurtsen Gert J. , Hollak Carla E. M. , Bosch Annet M. 
Published: January 2024

AbstractClassical galactosemia (CG) is an autosomal recessive disorder of galactose metabolism. Despite early initiation of a galactose‐restricted diet, patients develop long‐term complications including cognitive impairment. There is an ongoing debate whether the cognitive impairment in CG is stable throughout life or progresses with age. Earlier cross‐sectional and longitudinal studies regarding intelligence suggest stability, but longitudinal neuropsychological studies focusing on specific cognitive functions are limited. Therefore, the aim of this study is to assess cognitive change over time in adult CG‐patients. Ten adult patients with normal to borderline intelligence (mean age 33 years, range 22–49; IQ≥70 or independent work‐ or living situation) were assessed twice with a mean time interval of 3 years and 9 months (range 1023–1575 days). The neuropsychological assessments covered information processing speed, executive functioning, verbal fluency, and visuospatial functioning. Results showed that there was no significant decline or improvement in test scores on all neuropsychological measures except a decline on the Trail Making Test‐A (p = 0.048). However, this group‐level difference was subject to “regression to the mean” and was not endorsed by significant change in test scores measuring the same cognitive domain. Moreover, no specific pattern of reliable change (RCI > ‐1.96) was present on specific measures or within individual patients. This explorative study performed in 10 adult CG‐patients with normal to borderline intelligence revealed no cognitive change on several cognitive domains. This implies that the subset of adults with a normal to borderline IQ in their early and middle adulthood are cognitively stable.
Ontogenetic shifts in body form in the bull shark Carcharhinus leucas
Authors: Gayford Joel H. , Whitehead Darren A. , Jaquemet Sébastien 
Published: January 2024

AbstractRecent studies have uncovered mosaic patterns of allometric and isometric growth underlying ontogenetic shifts in the body form of elasmobranch species (shark and rays). It is thought that shifts in trophic and spatial ecology through ontogeny drive these morphological changes; however, additional hypotheses relating to developmental constraints have also been posed. The bull shark (Carcharhinus leucas) is a large‐bodied coastal shark that exhibits strong ontogenetic shifts in trophic and spatial ecology. In this study, we utilise a large data set covering a large number of morphological structures to reveal ontogenetic shifts in the body form of C. leucas, stratifying analyses by sex and size classes to provide fine‐scale, more ecomorphologically relevant results. Our results indicate shifts in functional demands across the body through ontogeny, driven by selective pressures relating to trophic and spatial ecology driving the evolution of allometry. We also find significant differences in scaling trends between life stages, and between the sexes, highlighting the importance of utilising large, diverse datasets that can be stratified in this way to improve our understanding of elasmobranch morphological evolution. Ultimately, we discuss the implications of these results for existing ecomorphological hypotheses regarding the evolution of specific morphological structures, and pose novel hypotheses where relevant.
Validity of individual self‐report oral health measures in assessing periodontitis for causal research applications
Authors: Bond Julia C. , Casey Sharon M. , McDonough Robert , McLone Suzanne G. , Velez Mabeline , Heaton Brenda 
Published: January 2024

AbstractBackgroundSelf‐reported measures of periodontitis developed for use in population surveillance are increasingly used in causal research. Numerous studies evaluate the validity of these measures against clinical parameters of periodontitis, yet few include validation parameters outside of multivariable models. Individual item validity is necessary to adequately inform use of these measures in causal research.MethodsWe used data from the National Health and Nutrition Examination Survey 2011‐2014 in which dentate participants completed full‐mouth periodontal examinations (N = 6966). We evaluated six self‐report questionnaire items related to periodontal disease status against periodontitis case definitions developed by the Centers for Disease Control and Prevention and American Academy of Periodontology (CDC‐AAP). We estimated the sensitivity and specificity of individual items using severe and moderate‐to‐severe periodontitis classifications. We additionally combined items to evaluate the validity of joint measures.ResultsSensitivity was highest when measures were evaluated against severe periodontitis. Self‐rated oral health of fair/poor demonstrated the highest sensitivity for severe (0.60) and moderate‐to‐severe periodontitis (0.48). Specificity was highest when evaluated against moderate‐to‐severe periodontitis, with self‐reported history of tooth mobility as the most specific measure (0.87 for severe disease; 0.92 for moderate‐to‐severe) followed by a history of bone loss (0.88 for severe; 0.91 for moderate‐to‐severe). Combining questions generally improved specificity at the expense of sensitivity.ConclusionsOur findings related to item‐specific validity and the associated clinical profiles facilitate needed considerations for the use of self‐reported measures of periodontitis in causal research. Additionally, item‐specific validity can be used to inform assessments of misclassification bias within such investigations.
BST2 promotes gastric cancer metastasis under the regulation of HOXD9 and PABPC1
Authors: Li Jiaying , Yang Ping , Hong Linjie , Xiao Wushuang , Zhang Luyu , Yu Zhen , Zhang Jieming , Pei Miaomiao , Peng Ying , Wei Xiangyang , Wu Xiaosheng , Tang Weimei , Zhao Yingying , Yang Juanying , Lin Zhizhao , Jiang Ping , Xiang Li , Zhang Hui , Lin Jianjiao , Wang Jide 
Published: January 2024

AbstractGastric cancer (GC) constitutes substantial cancer mortality worldwide. Several cancer types aberrantly express bone marrow stromal cell antigen 2 (BST2), yet its functional and underlying mechanisms in GC progression remain unknown. In our study, RNA sequencing data revealed that BST2 was transcriptionally activated by homeobox D9 (HOXD9). BST2 was significantly upregulated in GC tissues and promoted epithelial–mesenchymal transition and metastasis of GC. BST2 knockdown reversed HOXD9's oncogenic effect on GC metastasis. Moreover, BST2 messenger RNA stability could be enhanced by poly(A) binding protein cytoplasmic 1 (PABPC1) through the interaction between BST2 3′‐UTR and PABPC1 in GC cells. PABPC1 promoted GC metastasis, which BST2 silencing attenuated in vitro and in vivo. In addition, positive correlations among HOXD9, BST2, and PABPC1 were established in clinical samples. Taken together, increased expression of BST2 induced by HOXD9 synergizing with PABPC1 promoted GC cell migration and invasion capacity.