bluuebunny/crossref_metadata_2025_split huggingface dataset:
DEVELOPMENT OF COMPOSITE MATERIALS WITH INCREASED STABILITY FOR ENERGY-GENERATING AND ENERGY-SAVING SYSTEMS
Authors: Torchyniuk Pavlo , Lisovskyi Ivan , Belous Anatolii 
Published: January 2025

Techniques for creating composite organic-­inorganic materials by applying optically transparent polymers to the surface of the organic-inorganic perovskite CH3NH3PbI3 and composite cathode materials by modifying the surface of commercial cathode materials of the LiNixCoyAlzO2/LiNixMnyCozO2(NCA/NMC)type with nanoparticles of lithium oxide conductive material Li1.3Al .3Ti1.7(PO4)3 (LATP) have been developed.
It was shown that, depending on the type of polymer, CH3NH3PbI3 perovskite degrades due to the formation of different amounts of additional crystalline phases.It was established that the degree of degradation of the perovskite (1:2) CH3NH3PbI3 film is 100% after 220 days of influence to moisture and oxygen. Under the influence of moisture and oxygen for 500 days, perovskite/polymer films: PVB, SOC, and PVDC degrade only by 1.55%, 1.65% and 3.5%, respectively, which is 65, 60 and 29 times less than for pure perovskite CH3NH3PbI3.
The dependence of the electrochemical characteristics of a layered cathode material containing NCA/NMCon the method for applying a protective layer (mechanical application and sol-gel method) of nanoparticles of the lithium-conducting material LATP with a NASICON structure to its surface has been studied.It was found that for composite ca­thode materials based on NMC 111, the capacity drop during 80 charge/discharge cycles is reduced by 10–14% compared to the initial NMC 111. At the same time, the suitability of the cathode material NMC 111m(T) for the rapid charge/discharge process is 4–6% hi­gher than that of the initial cathode material.It is shown that both investigated methods of creating composite materials based on cathode materials with a high nickel content (NMC 811 and NCA) and LATP nanoparticles do not lead to improvement of their electrochemical cha­racteristics.
Lyrik: Stig Dagerman
Authors: Dagerman Stig 
Published: January 2025

Stig Dagerman 1923–1954). Författarskapet kom att bli ett av det svenska 40–talets mest tongivande, mångfacetterade. Under sin korta tid som författare lämnade han stora avtryck, både i Sverige och många andra länder. Debuten romanen Ormen (1945) fick ett omedelbart genombrott. Efter det publicerande han romanerna Bränt barn (1948) och Bröllopsbesvär (1949). Reportageboken Tysk höst är en samling texter skrivna under en resa, gjord för Expressens räkning genom det av kriget härjade Tyskland. Dagerman var också verksam som kulturskribent, poet, essäist, debattör och recensent. På tidningen Arbetaren skrev han dagverser – Dagermans dagsedlar. Dessa handlade om aktuella frågor, problem eller händelser. Sammanlagt skrev han omkring 1350 stycken. Redaktionen har för detta specialnummer valt att publicera följande två på tema fattigdom (Tryckeri AB Federativ, 1954, andra utgåvan).
Det sista Stig Dagerman skrev blev dagsedeln ”Varning för hunden!”. Dikten lämnades in till Arbetarens redaktion dagen före självmordet. Publicerades den 5 november 1954, samma dag som beskedet om hans död kom.
Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats
Authors: Li Qingqing , Zhang Fengrong , Li Xianyu , Wang Qing 
Published: January 2025

IntroductionBlood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available for the diagnosis stroke. Nervonic acid (NA), a vital long-chain monounsaturated fatty acid found in mammalian nervous tissue, shows promising therapeutic potential in neurological disorders. This study aimed to develop a reliable methodology for whole blood proteomics to identify early warning biomarkers and evaluate drug treatment efficacy.MethodsAfter modeling via the classic thread embolization method, whole blood samples were collected from the rats. Morphological assessments of brain tissue indicated that NA significantly mitigated brain and neuronal damage in rats. The differential protein expression profile was analyzed using Liquid Chromatography—Tandem Mass Spectrometry (LC-MS/MS) whole blood proteomics.ResultsZZZGene Ontology (GO) analysis revealed that, compared to ginkgo biloba extract (EGb), the proteins differentially expressed under NA intervention were predominantly involved in oxidative stress response and calcium-dependent adhesion processes. Key targets of NA in the treatment of middle cerebral artery occlusion (MCAO) models included ENO1, STAT3, NME2, VCL, and CCT3.DiscussionThis whole blood proteomic approach provides a comprehensive understanding of protein profiles associated with disease states, offering valuable insights into potential therapeutic targets and enabling the evaluation of NA and EGb intervention efficacy. Our findings underscore the protective effects of NA against cerebral ischemia-reperfusion injury and highlight its potential as a treatment for stroke.
Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses
Authors: Yang Dequan , Ma Li , Yang Zhongping , Yang Xianchao , Wang Jian , Ju Houbin , Lu Chunguang , Weng Yonggang , Zhao Heping , Shen Haixiao , Li Xin , Ge Feifei , Wang Xiaoxu , Wu Xiujuan , Xiang Meng , Feng Guidan , Tang Congsheng , Huang Shixin , Zhao Hongjin 
Published: January 2025

IntroductionViral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens often overlap, complicating accurate diagnosis; thus, there is an urgent need for rapid and precise diagnostic methods to improve prevention and treatment efforts. In this study, we developed a one-step multiplex reverse-transcription quantitative real-time polymerase chain reaction (mRT-qPCR) that enables the simultaneous detection of three key viral pathogens responsible for calf diarrhea: bovine kobuvirus (BKoV), bovine astrovirus (BoAstV), and bovine torovirus (BToV). However, development of accurate and rapid methods to distinguish these three viruses is helpful for the early detection, disease surveillance, and control of viral calf diarrhea.MethodsSpecific primers and minor groove binder (MGB)-based probes were designed targeting the 3D region of BKoV, ORF1 region of BoAstV, and N region of BToV. The sensitivity, specificity, and reproducibility ability were evaluated for the mRT-qPCR. Further, 80 bovine fecal samples were subjected to the mRT-qPCR, and the results were verified using conventional reverse-transcription PCR (RT-PCR) or PCR methods and sequencing methods.ResultsThis novel method demonstrated high sensitivity and specificity,achieving a detection limit of 24 copies/mL for each pathogen. Furthermore, the assay exhibited excellent reproducibility, with coefficients of variation below 1.5%, a strong linear correlation (R2 &gt; 0.996), and an amplification efficiency between 90% and 110%. Validation with 80 clinical samples from both diarrheic and non-diarrheic cattle across four farms in Shanghai showed a high degree of concordance with RT-PCR, with positive detection rates for BKoV, BoAstV, and BToV at 28.75%, 8.75%, and 3.75%, respectively, highlighting the predominance of BKoV and BoAstV. Notably, this study represents the first identification of BKoV, BoAstV, and BToV in the Shanghai region.DiscussionThe mRT-qPCR is a robust, rapid, and simple tool for identifying viral pathogens associated with calf diarrhea, facilitating the development of effective prevention and control measures that are vital for the future sustainability of the cattle industry.
Beyond the current state of just-in-time adaptive interventions in mental health: a qualitative systematic review
Authors: van Genugten Claire R. , Thong Melissa S. Y. , van Ballegooijen Wouter , Kleiboer Annet M. , Spruijt-Metz Donna , Smit Arnout C. , Sprangers Mirjam A. G. , Terhorst Yannik , Riper Heleen 
Published: January 2025

BackgroundJust-In-Time Adaptive Interventions (JITAIs) are interventions designed to deliver timely tailored support by adjusting to changes in users' internal states and external contexts. To accomplish this, JITAIs often apply complex analytic techniques, such as machine learning or Bayesian algorithms to real- or near-time data acquired from smartphones and other sensors. Given the idiosyncratic, dynamic, and context dependent nature of mental health symptoms, JITAIs hold promise for mental health. However, the development of JITAIs is still in the early stages and is complex due to the multifactorial nature of JITAIs. Considering this complexity, Nahum-Shani et al. developed a conceptual framework for developing and testing JITAIs for health-related problems. This review evaluates the current state of JITAIs in the field of mental health including their alignment with Nahum-Shani et al.'s framework.MethodsNine databases were systematically searched in August 2023. Protocol or empirical studies self-identifying their intervention as a “JITAI” targeting mental health were included in the qualitative synthesis if they were published in peer-reviewed journals and written in English.ResultsOf the 1,419 records initially screened, 9 papers reporting on 5 JITAIs were included (sample size range: 5 to an expected 264). Two JITAIs were for bulimia nervosa, one for depression, one for insomnia, and one for maternal prenatal stress. Although most core components of Nahum-Shani's et al.'s framework were incorporated in the JITAIs, essential elements (e.g., adaptivity and receptivity) within the core components were missing and the core components were only partly substantiated by empirical evidence (e.g., interventions were supported, but the decision rules and points were not). Complex analytical techniques such as data from passive monitoring of individuals' states and contexts were hardly used. Regarding the current state of studies, initial findings on usability, feasibility, and effectiveness appear positive.ConclusionsJITAIs for mental health are still in their early stages of development, with opportunities for improvement in both development and testing. For future development, it is recommended that developers utilize complex analytical techniques that can handle real-or near-time data such as machine learning, passive monitoring, and conduct further research into empirical-based decision rules and points for optimization in terms of enhanced effectiveness and user-engagement.
Impacts of stem cells from different sources on wound healing rate in diabetic foot ulcers: a systematic review and meta-analysis
Authors: Tong Le , Tang Lin , Tang Bangli , Zhang Jianna 
Published: January 2025

BackgroundDiabetic foot ulcers (DFU) are a significant complication of diabetes, with huge implications on patient morbidity and healthcare costs. The objective of this meta-analysis was to evaluate the impacts of stem cells from different sources on wound healing rate in DFU patients.MethodsWe systematically retrieved records via key databases PubMed, Cochrane Library, Web of Science, Embase, China National Knowledge Infrastructure (CNKI) and Wanfang from the inception to October 2024. The Stata 16.0 (Stata Corp, TX) software was used to perform the meta-analysis. Risk of bias in all included studies was evaluated by Cochrane Risk of Bias version 2.ResultsA total of 24 studies involving 1,321 patients were included. There was an increased likelihood of wound healing with peripheral blood-derived stem cells, the most effective cells (odds ratios (OR) = 7.31, 95% CI: 2.90–18.47), followed by adipose-derived stem cells (OR = 5.23, 95% CI: 2.76–9.90), umbilical cord-derived stem cells (OR = 4.94, 95% CI: 0.61–40.03), bone-derived stem cells (OR = 4.36, 95% CI: 2.43–7.85) and other sources stem cells (OR = 3.16, 95% CI: 1.83–5.45). Nevertheless, only umbilical cord-derived stem cells showed statistical significance (p &lt; 0.05). The heterogeneity ranged from non-existent in the adipose and peripheral blood groups (I2 = 0.00%) to moderate in the bone groups (I2 = 26.31%) and other groups (I2 = 30.62%), and substantial in the umbilical cord groups (I2 = 88.37%). Asymmetrical funnel plots pointed to publication bias, but the trim-and-fill method to correct for this brought the effect estimates even lower: based on the pooled OR, corrected OR was 3.40 (95% CI 2.39–4.84). Stem cell therapy was also associated with improvements in several secondary outcomes, suggesting its potential to influence the progression of DFU.ConclusionOur study suggested that stem cells from different sources showed potential in promoting wound healing in DFU, although with some variation in effectiveness. Despite some publication bias and moderate heterogeneity, the overall therapeutic effect remained positive. These findings indicated that stem cell therapy might influence the progression of DFU.
Proteomics profiling of inflammatory responses to elexacaftor/tezacaftor/ivacaftor in cystic fibrosis
Authors: Ozuna Hazel , Bojja Dinesh , Partida-Sanchez Santiago , Hall-Stoodley Luanne , Amer Amal , Britt Rodney D. , Sheikh Shahid , Frank David A. , Wang Weiyuan , Kang Bum-Yong , Miralda Irina , Durfey Samantha L. , Kopp Benjamin T. 
Published: January 2025

BackgroundCFTR modulator therapies have positive clinical outcomes, yet chronic inflammation and bacterial infections persist in people with CF (pwCF). How elexacaftor–tezacaftor–ivacaftor (ETI) fails to improve innate immune signaling responsible for bacterial clearance and inflammation resolution remains unknown.MethodsWe used an unbiased proteomics approach to measure the effect of ETI on inflammatory proteins. Plasma from 20 pediatric pwCF and 20 non-CF (NCF) was collected during routine examination and 3 months after ETI initiation. Protein screening was performed with an inflammation panel (Target 96, Olink®). Bioinformatics analysis was used to determine changes in protein expression.ResultsThere were significantly fewer pulmonary exacerbations after ETI initiation, along with sustained improvement in lung function and reduced bacterial colonization. Unpaired analysis of CF pre-ETI and NCF resulted in 34 significantly different proteins. Of these, CCL20, MMP-10, EN-RAGE, and AXIN1 had a log2 fold change of 1.2 or more. There was a modest shift in overall CF protein profiles post-ETI toward the NCF cluster. Unpaired analysis of protein differential expression between NCF and CF post-ETI identified a total of 24 proteins significantly impacted by ETI therapy (p-value ≤ 0.05), with only CCL20 having a log2 fold change higher than 1.2. Paired analysis (CF pre- and CF post-ETI) of differential protein expression demonstrated significant expression changes of MMP-10, EN-RAGE, and IL-17A. Pathway analysis identified significantly impacted pathways such as the NF-κB pathway.ConclusionThis study showed that ETI in a pediatric cohort had a modest effect on several inflammatory proteins with potential as biomarkers. Pathways significantly impacted by ETI can be further studied for future therapies to combat persistent inflammation and dysregulated immunity.
Daily feeding frequency impacts muscle characteristics and fat deposition in finishing pigs associated with alterations in microbiota composition and bile acid profile
Authors: Hu Luga , Tang Huayu , Xie Zhaoxi , Yi Hongyu , Feng Lunjie , Zhou Pan , Zhang Yong , Liu Jingbo , Ao Xiang , Zhou Jianchuan , Yan Honglin 
Published: January 2025

IntroductionFeeding frequency has been shown to affect growth and body composition of the host associated with gut microbiota. It remains unknown whether adjusting feeding frequency could effectively regulate both skeletal muscle development and whole-body lipid metabolism and thus affect carcass composition and feed conversion efficiency. Therefore, this study aimed to explore the effects of feeding frequency on muscle growth, fat deposition, cecal microbiota composition, and bile acid composition in finishing pigs.MethodsSixteen Sichuan-Tibetan black pigs, with an initial weight of 121.50 ± 1.60 kg, were divided into two groups and fed either two meals (M2) or four meals (M4) per day. The trial lasted 30 days. The muscle fiber characteristics, lipid metabolism in adipose tissue, and cecal microbiota and bile acid composition were determined.ResultsThe present study revealed that pigs fed four meals exhibited a lower feed-to-gain ratio, abdominal fat weight, and average backfat thickness (p &lt; 0.05), as well as a higher loin eye area (p = 0.09) and myofiber diameter in the longissimus muscle than their counterparts. The mRNA expression of slow-twitch fiber and myogenesis-associated genes in the longissimus muscle was upregulated, while lipid metabolism-related genes in the backfat were downregulated in the M4 group compared to the M2 group (p &lt; 0.05). The M4 pigs exhibited higher abundances of Firmicutes, Actinobacteriota, Bacillus, Clostridium_sensu_1, and Romboutsia, and lower abundances of Spirochaetota, Verrucomicrobiota, Treponema, and Muribaculaceae in the cecal content than the M2 pigs (p &lt; 0.05). A higher feeding frequency increased the levels of primary bile acids and decreased the concentrations of taurine-conjugated bile acids in the cecal content of pigs (p &lt; 0.05).ConclusionOur research suggested that the M4 feeding pattern, compared to the M2 pattern, promoted muscle growth and reduced fat deposition by enhancing fast- to slow-twitch fiber conversion and myogenesis in the muscle and repressing lipid metabolism in adipose tissue, associated with altered microbiota composition and bile acid profiles.
Opportunities, challenges, and difficulties in NMR-based metabolomics applied to neovascular age-related macular degeneration (nAMD) patient follow-up
Authors: Schoumacher M. , Lambert V. , Campas M. , Blaise P. , Locht B. , Thys M. , Duchateau E. , Cavalier E. , Rakic J.-M. , Noël A. , de Tullio P. 
Published: January 2025

IntroductionThis study applies NMR-based metabolomics to investigate neovascular age-related macular degeneration (nAMD), addressing challenges in patient management, disease progression evaluation, and treatment response assessment. A two-year follow-up of 29 nAMD patients undergoing treatment provided 231 time points for analysis.MethodsOver the two-year period, 11 males and 18 females (aged 61–92 years) were monitored, yielding 231 time points. At each time point, blood samples for NMR metabolomics analysis, clinical measurements (e.g., lactate, glucose levels, HDL/LDL cholesterol, and blood pH), and optical coherence tomography (OCT) images were collected to evaluate the progression of choroidal neovascularization. 1H-NMR metabolomic analysis led to the quantification of over 60 metabolites and of the major lipoprotein fractions. Both multivariate and univariate statistical approaches tailored for longitudinal data were employed to identify biomarkers correlating metabolomic changes with ocular alterations during disease progression.Results and DiscussionDespite a rigorous analytical workflow enabling precise quantification of over 60 metabolites and the application of advanced statistical tools for longitudinal data, achieving consistent results across the cohort proved challenging. The dataset’s heterogeneity, reflecting real-world clinical practice, complicated the derivation of global conclusions. Personalized analyses on a patient-by-patient basis successfully identified individual correlation models, but a universal model remained elusive. This study highlights the inherent challenges of translating findings from controlled settings into clinical practice, where factors such as visit frequency, treatment variability, and disease heterogeneity limit data uniformity. We emphasize the importance of experimental design in longitudinal studies, particularly when dealing with incomplete and variable datasets. We are therefore confident that, considering both the challenges and difficulties identified in this work and the preliminary results presented here, it is possible to develop predictive and individualized models for monitoring patients with nAMD. Such models could greatly assist clinicians in providing better care for these patients.
Do non-mammary conditions influence patients’ cosmetic perception after breast conserving surgery?
Authors: de Oliveira-Junior Idam , da Silva Fabíola Cristina Brandini , Sarri Almir José , Vieira René Aloísio da Costa 
Published: January 2025

IntroductionCompared to mastectomy, breast-conserving surgery (BCS) guarantees equivalent local control and survival, with lower morbidity and better quality of life (QOL), even in the long term. However, some BCS patients consider the cosmetic result to be unsatisfactory, which may affect QOL.Material and methodsThis prospective, cross-sectional study included patients who underwent BCS. The patients answered the European Organization for Research and Treatment of Cancer Quality of Life questionnaire (EORTC QLQ) - C30, EORTC QLQ-BR23 and Breast Cancer Treatment Outcome Scale (BCTOS) questionnaires, underwent cosmetic breast self-assessment and had their breasts photographed. The photographs were analyzed using Breast Cancer Conservative Treatment. Cosmetic results (BCCT.core). For the categorical variables, the frequencies were calculated; for the numerical variables, the mean and standard deviation. The BCCT.core results were compared with the cosmetic results of the patients, which yielded four possible results: concordant satisfaction, discordant satisfaction, concordant dissatisfaction and discordant dissatisfaction (satisfactory BCCT.core evaluation but patient dissatisfaction). The kappa test was used for agreement between categorical variables. Student’s t test and Mann-Whitney were used to assess the relationship between QOL and cosmetic results. The ANOVA were performed with the adjusted Bonferroni correction to compare the four groups.ResultsA total of 300 patients were evaluated, 298 underwent self-assessment of the breasts (76.8% satisfactory results and 23.2% unsatisfactory) and 297 underwent BCCT.core evaluation (29.9% satisfactory results and 79.1% unsatisfactory), which had a kappa of 0.095 (p = 0.01). In the self-assessment, patients with unsatisfactory cosmetic results had worse overall health, physical, functional, emotional, cognitive, and social capacity, fatigue, pain, dyspnea, financial difficulties, body image; future prospects, side effects, breast symptoms, functional aspects, cosmetics and edema. When we used software evaluation, these relationships did not have the same proportions. In patients with “discordant dissatisfaction”, higher pain scores and worse functionality on the treated side were found.ConclusionAn unsatisfactory cosmetic result was associated with worse QOL, which may be associated with other factors, such as breast pain and functionality.