bluuebunny/crossref_metadata_2025_split huggingface dataset:
Island area, isolation and climate effects upon flower traits in a megadiverse archipelago
Authors: Trethowan Liam A. , Jennings Laura , Bramley Gemma , Briggs Marie , Clark Ruth , Dawson Sally , Fontaine Max , Heatubun Charlie , Lewis Gwilym , Lucas Eve , Moore Alison , Pearce Laura , Puglisi Carmen , Utteridge Timothy M. A. , Wanma Jimmy , Rustiami Himmah 
Published: October 2023

AbstractAimFlower traits are critical to the mutualistic networks that underpin ecosystem function. However, the broad‐scale drivers of flower traits are unclear. Using the megadiverse islands of Malesia, we test three hypotheses: (1) Flower size, openness and colourfulness (i.e. the trend from white to colourful flowers) will decrease with increasing temperature, but not with increasing precipitation seasonality. (2) Flower size, openness and colourfulness will increase with island area, and decrease with isolation. (3) Models parameterised by climate, rather than non‐climatic island characteristics will have the greatest predictive capacity as climate affects both pollinator communities and energetic/physiological constraints upon flower traits.LocationFourteen Malesian islands.TaxonAngiosperms.MethodsWe applied Bayesian phylogenetic models of occurrence for >2500 species in 400 m elevation bands, across islands, as a function of flower trait interactions with either climatic variables or islands characteristics. We compare predictive capacity of models based on climate versus island characteristics using Leave One Out‐Cross Validation.ResultsAll six flower traits examined varied with at least temperature, precipitation seasonality, island area and isolation. Flowers are larger in cold environments. The lowland tropics are mostly inhabited by white flowered species. At cold high elevations, red and pink flowered species are more frequent whereas green and purple flowered species increase in the drought‐prone seasonally dry tropics. Flower openness declined with precipitation seasonality and increased with isolation. Models parameterised by climate performed best for perianth length, red and white flowers, whereas pink flowers and flower openness were better predicted using island characteristics.Main ConclusionsThere are relationships between flower traits and climatic gradients within Malesia. However, island characteristics have also left a legacy upon current flower trait distributions. These island biogeographical impacts are likely key for the eco‐evolutionary drivers of flower traits.
Integrating bulk and single‐cell data to predict the prognosis and identify the immune landscape in HNSCC
Authors: Yang Chunlong , Cheng Xiaoning , Gao Shenglan , Pan Qingjun 
Published: October 2023

AbstractThe complex interplay between tumour cells and the tumour microenvironment (TME) underscores the necessity for gaining comprehensive insights into disease progression. This study centres on elucidating the elusive the elusive role of endothelial cells within the TME of head and neck squamous cell carcinoma (HNSCC). Despite their crucial involvement in angiogenesis and vascular function, the mechanistic diversity of endothelial cells among HNSCC patients remains largely uncharted. Leveraging advanced single‐cell RNA sequencing (scRNA‐Seq) technology and the Scissor algorithm, we aimed to bridge this knowledge gap and illuminate the intricate interplay between endothelial cells and patient prognosis within the context of HNSCC. Here, endothelial cells were categorized into Scissorhigh and Scissorlow subtypes. We identified Scissor+ endothelial cells exhibiting pro‐tumorigenic profiles and constructed a prognostic risk model for HNSCC. Additionally, four biomarkers also were identified by analysing the gene expression profiles of patients with HNSCC and a prognostic risk prediction model was constructed based on these genes. Furthermore, the correlations between endothelial cells and prognosis of patients with HNSCC were analysed by integrating bulk and single‐cell sequencing data, revealing a close association between SHSS and the overall survival (OS) of HNSCC patients with malignant endothelial cells. Finally, we validated the prognostic model by RT‐qPCR and IHC analysis. These findings enhance our comprehension of TME heterogeneity at the single‐cell level and provide a prognostic model for HNSCC.
Analysis of the effectiveness of PDT with 5‐aminolevulinic acid in comparison to blue/red light combined with intralesional triamcinolone injection in treatment of severe inflammatory acne: A retrospective study
Authors: Hu Yi , Cheng Yang , Jie Wang 
Published: October 2023

AbstractObjectiveIn this study, the therapeutic effect of 5‐aminolevulinic acid photodynamic therapy (ALA‐PDT) in comparison to blue/red light combined with intralesional triamcinolone injection for severe inflammatory acne was evaluated and analyzed.MethodsOne hundred and four cases of severe inflammatory acne were analyzed in this study. They were divided into two groups as control and observation groups, 52 cases in each group. The control group (group A) received red and blue light combined with triamcinolone injection and lidocaine injection (1:4), while the observation group (Group B) was treated with ALA‐PDT. Finally, the therapeutic effect and the occurrence of adverse reactions were compared between the two groups.ResultsAfter 2, 4 and 6 weeks, the effectiveness rates of group B was 28.85%, 75.00%, and 86.54%, respectively while it was 9.62%, 51.92%, and 69.23%, respectively in group A. The difference between A and B was statistically remarkable (χ2 = 6.1905, 5.9713, 4.5217, p = 0.0128, 0.0145, 0.0335 at p < 0.05). In addition, the incidence of adverse reactions in B was 5.77%, lower than A (32.69%). This difference between A and B was statistically remarkable (χ2 = 12.1333, p = 0.0005). After 2, 4, and 6 weeks of treatment, the number of residual lesions in the group B group was remarkably lower than group A (p < 0.01). There was remarkable difference in the incidence of pain, burning sensation, pigmentation and erythema between the two groups.ConclusionsThe therapeutic effect of ALA‐PDT in the treatment of severe acne is better than red blue light combined with triamcinolone injection and lidocaine injection. In addition, ALA‐PDT has an ideal effect in the treatment of severe acne.
Aestheticizing the Pain: A Critical Analysis of Media Representation of Earthquake Victim Children in Turkey
Authors: Atalay Gül Esra , Muratoğlu Pehlivan Bahar 
Published: October 2023

AbstractOn February 6, 2023, earthquakes with magnitudes of 7.7 and 7.6 struck southern and central Turkey. The disaster caused severe damage to buildings and resulted in the loss of thousands of lives, leaving many children injured, traumatized, and without basic needs. One of the most sensitive issues was the news stories about children rescued from the rubble. These news stories were highly emotional and sensational, frequently describing the children as miracles. However, they often disregarded the reasons and negligence that caused their victimization. The journalism ethics related to the representation of children, disaster coverage, and interviewing victims are also ignored. This study uses a multimodal critical discourse analysis method to explore the discursive strategies employed in the media coverage of this topic. Three salient common themes were included in the study: “Miracles and hope”, “patriarchal state”, and “sensational/dramatic elements. The analysis shows that media representations of earthquake victim children in Turkey aestheticize their pain and suffering, reducing them to objects of pity or spectacle. By examining these representations critically, the study aims to raise awareness about the importance of ethical and responsible media practices in reporting on natural disasters and other humanitarian crises.
Affect in cross‐chronotope alignments in narrations about Aristides de Sousa Mendes and their subsequent circulations
Authors: Koven Michele 
Published: October 2023

AbstractThis article analyzes the role of emotion in narrations about the past, understandable as familial, intergenerational, or national. I examine how participants report and display affect in narratives about Aristides de Sousa Mendes, the Portuguese consul of Bordeaux who issued thousands of lifesaving visas in June of 1940. Three sets of participants (descendants of visa recipients, Sousa Mendes' descendants, and Portuguese institutional representatives) each explicitly report and implicitly display how the Sousa Mendes story moves them emotionally. I then discuss how the emotion in these narratives may be circulated and taken up by broader audiences. Building on Irvine's discussion of the heteroglossia of affective expression (1990), participants may attribute emotion to others, signal emotion as occurring in the present or in a prior space–time, or merge emotional past and present in various types of emotional “reliving.” By treating emotion as eventlike, it can thus be considered chronotopic. I analyze the relationships between (re)presentation of emotion across multiple narrated and narrating chronotopes. This approach reveals how differently positioned participants' cross‐chronotope alignments yield particular types of affective displays and experiences that others can then take up and recontextualize.
Development of a colorimetric loop‐mediated isothermal amplification (LAMP) assay to facilitate monitoring of carbendazim‐resistant isolates of Colletotrichum truncatum based on the β‐tubulin E198A mutation
Authors: Poti Teeranai , Nalumpang Sarunya , Akimitsu Kazuya 
Published: October 2023

AbstractAnthracnose, a disease mainly caused by Colletotrichum truncatum, is a serious threat to vegetable soybean production worldwide. Carbendazim, a methyl‐benzimidazole carbamate fungicide, has been frequently applied to control the disease. Unfortunately, carbendazim‐resistant isolates of C. truncatum have emerged, resulting in inefficient disease control. Therefore, a rapid and simple method for monitoring the fungicide‐resistant isolates in fungal populations is essential to select the most appropriate strategy to control the disease and to handle fungicide resistance in a timely manner. In this study, we developed a colorimetric loop‐mediated isothermal amplification (LAMP) assay to detect carbendazim‐resistant isolates by using the E198A mutation in the TUB2 gene as the marker. With the optimal primers, the developed LAMP assay could detect resistant isolates after incubation for 40 min at 67°C. The results could be simply observed with the naked eye, and the assay provided 10‐fold higher sensitivity than conventional PCR. Moreover, the specificity, accuracy, and reproducibility of the generated LAMP were shown in testing with 85 C. truncatum isolates from five locations in northern Thailand. Resistant isolates carrying the F200Y mutation were not detected with the LAMP assay. Thus, the developed colorimetric LAMP assay could be used as a rapid and simple detector of certain carbendazim‐resistant isolates of C. truncatum.
Tolerability of naso‐esophageal feeding tubes in dogs and cats at home: Retrospective review of 119 cases
Authors: Dumont Renaud , Lemetayer Julie , Desquilbet Loïc , Darnis Elodie 
Published: October 2023

AbstractBackgroundThe use of naso‐esophageal feeding tubes (NFT) at home could represent an alternative way to reduce the costs for owners and facilitate enteral feeding until recovery of a spontaneous appetite.ObjectiveTo describe the use of NFT at home in dogs and cats and evaluate the satisfaction of owners and their capacity to handle the device.AnimalsOne hundred nineteen client‐owned animals (90 cats and 29 dogs) which remained anorexic during hospitalization and were discharged with NFT for at least 24 hours after placement.MethodsMedical records were reviewed retrospectively, and owners were contacted by telephone calls. Complications were reported according to their relative severity (minor and major). Owners were asked to report their experience and comfort with NFT management.ResultsNaso‐esophageal feeding tubes were kept in place at home for a median of 6 days (range, 1‐17) and 62.2% (95% confidence interval [CI]: 53.3‐70.7) of animals recovered a spontaneous appetite while wearing NFT, 60% (95% CI: 44.4‐75.6) of the remaining animals recovered a spontaneous appetite after removal. Overall complication rate was 65.5% (95% CI: 57.0‐74.0), but only 18.5% (95% CI: 11.5‐25.5) required a consultation and no life‐threatening complication occurred. Owners were satisfied in 94.1% (95% CI: 89.9‐98.3) of cases.Conclusion and Clinical ImportanceAlthough most animals discharged with NFT at home presented complications, no major adverse effects were reported and NFT were easily handled by owners. This study provides evidence that NFT can be well tolerated at home.
Al‐Cu‐Fe alloys in the solar system: Going inside a Khatyrka‐like micrometeorite (KT01) from the Nubian Desert, Sudan
Authors: Ma Chi , Hu Jinping , Suttle Martin D. , Guan Yunbin , Sharp Thomas G. , Asimow Paul D. , Steinhardt Paul J. , Bindi Luca 
Published: October 2023

AbstractA recently described micrometeorite from the Nubian desert (Sudan) contains an exotic Al‐Cu‐Fe assemblage closely resembling that observed in the Khatyrka chondrite (Suttle et al., 2019; Science Reports 9:12426). We here extend previous investigations of the geochemical, mineralogical, and petrographic characteristics of the Sudan spherule by measuring oxygen isotope ratios in the silicate components and by nano‐scale transmission electron microscopy study of a focused ion beam foil that samples the contact between Al‐Cu alloys and silicates. O‐isotope work indicates an affinity to either OC or CR chondrites, while ruling out a CO or CM precursor. When combined with petrographic evidence we conclude that a CR chondrite parentage is the most likely origin for this micrometeorite. SEM and TEM studies reveal that the Al‐Cu alloys mainly consist of Al metal, stolperite (CuAl), and khatyrkite (CuAl2) together with inclusions in stolperite of a new nanometric, still unknown Al‐Cu phase with a likely nominal Cu3Al2 stoichiometry. At the interface between the alloy assemblage and the surrounding silicate, there is a thin layer (200 nm) of almost pure MgAl2O4 spinel along with well‐defined and almost perfectly spherical metallic droplets, predominantly iron in composition. The study yields additional evidence that Al‐Cu alloys, the likely precursors to quasicrystals in Khatyrka, occur naturally. Moreover, it implies the existence of multiple pathways leading to the association in reduced form of these two elements, one highly lithophile and the other strongly chalcophile.
Prior selection of itraconazole in the treatment of recalcitrant Trichophyton indotineae infection: Real‐world results from retrospective analysis
Authors: Song Ge , Kong Xue , Li Xiaofang , Liu Weida , Liang Guanzhao 
Published: October 2023

AbstractBackgroundThe number of terbinafine‐resistant Trichophyton indotineae is increasing in recent years while the treatment is still a matter to discuss.ObjectivesTo explore the best therapeutic approach, we present real‐world treatment of T. indotineae infection by analysing publicly available data.MethodsWe have reviewed all published articles, mainly including case reports and case series, on the drug‐resistant T. mentagrophytes complex by using the key search terms to search the databases.ResultsWe enrolled 25 articles from 14 countries, including 203 times of treatment information for 113 patients. The cure rate of itraconazole 200 mg per day at the fourth, eighth and the twelfth week were 27.27%, 48.48% and 54.55%, respectively, which was significantly higher than terbinafine 250 mg per day (8.77%, 24.56% and 28.07%) and even 500 mg/d terbinafine. Griseofulvin 500–1000 mg for 2–6 months may be effective while fluconazole had no record of successful treatment. Voriconazole and ravuconazole had potential therapeutic efficacy. Topical therapy alone showed limited therapeutic efficacy, but the combination with oral antifungals can be alternative.ConclusionOral itraconazole 200 mg per day for 4–8 weeks was the most effective treatment out of these commonly used antifungal drugs, and can be prior selection.
Labelling of intravenous drug delivery devices in critically ill patients: A scoping review
Authors: Nunes Geovane de Kassio , Antunes Lucas Malta Souza , da Silva Rodrigo Nogueira , da Silva Rafael Celestino 
Published: October 2023

AbstractBackgroundLabelling is a strategy that contributes to the correct and faster identification of drugs, minimizing misidentification. There is a gap in knowledge on optimal labelling standards for intravenous (IV) devices applied to the care of critically ill patients.AimThe goal of this article was to map existing knowledge on the labelling of IV drug delivery devices in critically ill patients for the prevention of medication errors.Study DesignThis was a scoping review conducted according to the JBI methodology in the LILACS, MEDLINE, CINAHL, IBECS, Scopus, Embase and Web of Science databases, and on the websites of specialized institutions. Searches were conducted up to December 2022 for scientific articles and grey literature that addressed the labelling of IV devices in intensive care units, emergency departments, and anaesthesia units. The data were collected using a structured form and were later classified, summarized, and aggregated to map the knowledge related to the review question.ResultsTwenty‐one documents were included, which demonstrated variability in label use with IV drug delivery devices. The following features of structure and design stood out: printed format, colour coding, letter size differentiation, and the use of sturdy material. In terms of information, the name of the drug, dose, date and time of preparation, identification of the patient, and who prepared it were found.ConclusionsThe identified patterns contributed to the reduction of drug misidentification and the development of timelier drug labelling and administration.Relevance to Clinical PracticeThe evidence supports the development of standardized labels for the prevention of medication errors.
Comparison of wholemount dissection methods for neuronal subtype marker expression in the mouse myenteric plexus
Authors: Gomez‐Frittelli Julieta , Hamnett Ryan , Kaltschmidt Julia A. 
Published: October 2023

AbstractBackgroundAccurately reporting the identity and representation of enteric nervous system (ENS) neuronal subtypes along the length of the gastrointestinal (GI) tract is critical to advancing our understanding of ENS control of GI function. Reports of varying proportions of subtype marker expression have employed different dissection techniques to achieve wholemount muscularis preparations of myenteric plexus. In this study, we asked whether differences in GI dissection methods could introduce variability into the quantification of marker expression.MethodsWe compared three commonly used methods of ENS wholemount dissection: two flat‐sheet preparations that differed in the order of microdissection and fixation and a third rod‐mounted peeling technique. We also tested a reversed orientation variation of flat‐sheet peeling, two step‐by‐step variations of the rod peeling technique, and whole‐gut fixation as a tube. We assessed marker expression using immunohistochemistry, genetic reporter lines, confocal microscopy, and automated image analysis.Key Results and ConclusionsWe found no significant differences between the two flat‐sheet preparation methods in the expression of calretinin or neuronal nitric oxide synthase (nNOS) as a proportion of total neurons in ileum myenteric plexus. However, the rod‐mounted peeling method resulted in decreased proportion of neurons labeled for both calretinin and nNOS. This method also resulted in decreased transgenic reporter fluorescent protein (tdTomato) for substance P in distal colon and choline acetyltransferase (ChAT) in both ileum and distal colon. These results suggest that labeling among some markers, both native protein and transgenic fluorescent reporters, is decreased by the rod‐mounted mechanical method of peeling. The step‐by‐step variations of this method point to mechanical manipulation of the tissue as the likely cause of decreased labeling. Our study thereby demonstrates a critical variability in wholemount muscularis dissection methods.
Prognostic Factors and Surgical Impact of Non‐metastatic Conventional Chondrosarcoma of the Extremities
Authors: Wang Wenhui , Zhen Junping 
Published: October 2023

ObjectiveChondrosarcoma is a common bone malignancy, and the main treatment method is surgery. Different surgeries lead to different survival outcomes. The aim of this study was to construct a new clinical predictive tool to accurately predict the overall survival (OS) and cause specific survival (CSS) of patients with chondrosarcoma receiving different treatments.MethodsA total of 620 patients with chondrosarcoma registered between January 1, 2000 and December 31, 2016 were recruited as study targets. The missing values are filled by multiple imputation. Two continuous variables, age and tumor size, were divided into binary variables based on Kaplan–Meier curve. Univariate and multivariate analyses were used to explore predictors and establish nomograms. Propensity score matching (PSM) analysis was used to reduce the impact of potential confounders to determine whether different surgical modalities had any survival benefits in subgroups.ResultsIn a multivariate cox regression, age, grade, tumor size, radiotherapy, chemotherapy, and surgical methods were identified as independent prognostic factors for chondrosarcoma. To construct 1‐, 3‐, and 5‐year nomogram maps of OS and CSS with prognostic factors and verify the c index internally (OS, 0.807; CSS, 0.847) above American Joint Committee on Cancer (AJCC) (OS, 0.685; CSS, 0.732).ConclusionThis study found that the 5 year overall survival rate of patients with non‐metastatic chondrosarcoma of the extremities was about 80%. Age, high malignancy, large tumor, prior chemoradiotherapy, and poor surgical selection were independent risk factors. Therefore, the nomogram established in this study will help to optimize clinicians' personalized decision making for patients.
A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology
Authors: Calvache Camilo , Vazquez‐Vilar Marta , Moreno‐Giménez Elena , Orzaez Diego 
Published: October 2023

SummaryPlant Synthetic Biology aims to enhance the capacities of plants by designing and integrating synthetic gene circuits (SGCs). Quantitative reporting solutions that can produce quick, rich datasets affordably are necessary for SGC optimization. In this paper, we present a new, low‐cost, and high‐throughput reporter system for the quantitative measurement of gene expression in plants based on autonomous bioluminescence. This method eliminates the need for an exogenous supply of luciferase substrate by exploiting the entire Neonothopanus nambi fungal bioluminescence cyclic pathway to build a self‐sustained reporter. The HispS gene, the pathway's limiting step, was set up as the reporter's transcriptional entry point as part of the new system's design, which significantly improved the output's dynamic range and brought it on par with that of the gold standard FLuc/RLuc reporter. Additionally, transient ratiometric measurements in N. benthamiana were made possible by the addition of an enhanced GFP as a normalizer. The performance of new NeoLuc/eGFP system was extensively validated with SGCs previously described, including phytohormone and optogenetic sensors. Furthermore, we employed NeoLuc/eGFP in the optimization of challenging SGCs, including new configurations for an agrochemical (copper) switch, a new blue optogenetic sensor, and a dual copper/red‐light switch for tight regulation of metabolic pathways.