A multicentric and nationwide predictive study role of T cell sub‐population in the prevalence and prognosis of cryoglobulinemia among genotype 4 chronic hepatitis C patients
Authors: Abdel‐Samiee Mohamed , Youssef Mohamed I. , Elghamry Fathy , Bazeed Mahmoud , Al‐Shorbagy Mohamed , Shalaby Helmy , Shabana Hossam , Abdelsameea Eman , Lashin Hesham El Sayed , El Zamek Hossam M. Farid , Esam Tarek , Alwaseef Mohammad AbdElhameed Ahmed , Helmy Housam Ahmed , Almarshad Feras , Khalaf Fatma A. , Yossef Badawy W. AboBakr , Kassem Arafat , Gabr Basant Mostafa , Abdelfattah Ahmed , S. AboShabaan Hind , Aboufarrag Galal Abdelhameed , Omar Marwa M. , Bakeer Mohammed Saied , Imam Mohammed S. , Ibrahim Essam S. , Kamel Shimaa Y , Allisy Talaat , Mohammed Omima Sayed , Farahat Ali , El‐Khayat Mohsen M. , Sekeen Mohamed Abdelrafaa Hassan , Zaher Eman Mohammed , Said Ashraf , Abuamer Ahmed , Elmahdi Essam 
Published: December 2023

AbstractThe infection caused by the hepatitis C virus (HCV) is a significant global health concern. The prevailing genotype of HCV in Egypt is 4a, commonly referred to as GT‐4a. A significant proportion exceeding 50% of patients infected with HCV experience extrahepatic manifestations (EHMs), encompassing a diverse range of clinical presentations. These manifestations, including essential mixed cryoglobulinemia (MC), can serve as initial and solitary indicators of the disease. The complete understanding of the pathogenesis of EHM remains unclear, with autoimmune phenomena being recognized as the primary causative factor. In this study, we examined the predictive significance of T‐cell subpopulations in relation to the occurrence and prognosis of cryoglobulinemia in HCV patients. A total of 450 CHC genotype four treatment naïve patients were enrolled in this analytic cross‐sectional study after thorough clinical, laboratory, and radiological examinations. All patients underwent laboratory investigations, including testing for cryoglobulin antibodies and measurements of CD4 and CD8 levels; two groups were described according to their test results: Group 1 consists of patients who have tested positive for cryoglobulin antibodies and Group 2 consists of patients who have tested negative for cryoglobulin antibodies. The exclusion criteria encompassed individuals with HIV infection or chronic HBV infection. Additionally, pelvi‐abdominal ultrasonography was performed. Our study included 450 treatment naïve CHC patients (59% male, mean age 50.8 years). The patients were categorized according to their cryoglobulin antibodys test results into two groups: group A, CHC patients with cryoglobulin antibodies (Abs) negative (364 patients), and group B, CHC patients with cryoglobulin Ab positive (86 patients). Group B demonstrated a higher average age, elevated international normalized ratio, more prolonged duration of HCV infection, lower albumin, higher alanine aminotransferase, higher aspartate aminotransferase, higher bilirubin, lower CD8, lower CD4, and lower CD4:CD8 ratio. In contrast, 27 out of 86 (31.40%) patients in group B had symptoms; 85.8% had purpura and arthralgia, 74.3% had paresthesias, 86.7% had weakness, and 12.2% had non‐Hodgkin's lymphoma. The levels of CD4 and CD8 were found to be decreased in chronic HCV patients with MC. T‐cell subpopulation serves as a reliable indicator for assessing the prevalence and prognosis of MC in individuals with genotype 4 chronic hepatitis C. However, additional research is needed to further understand the development and spread of various emerging infectious diseases. Nevertheless, it is noteworthy that a critical threshold may exist beyond which EHM reaches a point of no return.