bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of Diagnostic Laboratory and Screening Strategy for Rodent Pathogens
Authors: Devan R K  Shakthi , Kishore Krishna , Kumar Nethaji , Kamath Gireesh , Poosala Suresh 
Published: January 2025

Health monitoring surveillance is an essential requirement of barrier facility as animal research is largely depends on health status of laboratory animals being used. Early identification of pathogens by using ideal methods can prevent infections; otherwise confounding variables can occur in the experimental results of biomedical research. Syngene has been importing animals from at least 12 different barriers facilities from USA/Europe/India to conduct planned experiments at appropriate ages. However, the limiting factor was stringent quarantine period (upto 21 days), which further increases age of the animals. In order to maintain the healthy animals, comprehensive testing strategies have been implemented in a programmed manner to identify infectious agents during early stages, thereby preventing the spread of contamination to other areas within the vivarium. The primary aim was to screen representative samples of each batch as per the schedule apart from established sentinel program which were further analyzed at 6, 12 and 18 weeks interval including comprehensive annual testing. Based on the inherent challenges, a diagnostic laboratory was established at site to screen pathogens using validated methods primarily by ELISA, IFA to detect antibodies and PCR for nucleic acid detection as well as rapid screening which has been a prerequisite for early quarantine release of animals for experiments. Alternatively, MFIA has been used as high throughput assay for rodent pathogens at periodical intervals along with other methods. In addition, bacterial pathogens if any can be identified by using VITEK apart from the routine contact plating of swab samples taken from animals. The in-house diagnostic laboratory including at least 4 outside laboratories have been employed for weekly and/or sentinel samples screening by serological assays such as ELISA, MFIA, IFA and molecular technique of PCR for rodent pathogens. Collectively, the robust screening systems at multi-site laboratories which eventually reduced turnaround time by adopting high throughput assay including confirmatory methods to obtain consistent and reliable results of rodent pathogens.
Breeding performance with special reference to pre-weaning mortality of C57BL/6 and B6D2F1 hybrid strain of mice maintained at ACTREC Animal Facility
Authors: Thorat Rahul , Ingle Arvind 
Published: January 2025

Successful breeding programme of laboratory animals is one of a key factor in any facility operations. In-house breeding of laboratory animal species depends on using correct breeding method, number of animals requested by the researchers, litter size, birth weight, perinatal mortality, weaning percentage, female:male ratio, parity interval, fostering, postpartum heat, non-productive breeders, enrichment programme etc. To fulfil the animal demands for in vivo research, these points play significant role, especially perinatal mortality. Perinatal mortality is strain dependent and therefore facility must be aware about the percentage of pups mortality to avoid further miscalculation while setting up the breeding program. In order to get the first hand information about strain wise perinatal mortality, historical control data or baseline data should be generated which play an important role in successful breeding programme and timely meeting the target of providing experimental animals. Aim of the present study was to generate baseline control values of reproductive efficiency parameters of commonly used mice strains at ACTREC Animal Facility. The existing in-house data was compiled for the period of July to December 2019. Perinatal mortality varied between the primiparous and multiparous females. In total, data of 4247 pups obtained from 39 inbred primiparous, 93 outbred primiparous, 159 inbred multiparous and 466 outbred multiparous females. The average number of pups delivered by inbred primiparous and multiparous females was recorded as 5.13 and 5.85, respectively. There was no significant difference between the female:male ratio between primiparous and multiparous females of C57BL/6 inbred strain. Weaning of pups was done on day 21. Percentages of weaning of pups from primiparous and multiparous inbred strain were recorded as 87 and 92.37%, respectively. Perinatal mortality in inbred primiparous female was 13% while in inbred multiparous was 7.63%. It is observed that perinatal mortality in inbred primiparous pups is higher than inbred multiparous females. In case of outbred stock of C57BL/6 mice, we observed average litter born of 4.96 and 5.70 for primiparous and multiparous mothers, respectively. Weaning percentage of 83.73% and 93.56% was recorded in outbred primiparous and multiparous mothers, respectively. Perinatal mortality in pups obtained from outbred primiparous and multiparous mothers was 15.84% and 6.44% respectively. The data indicated that perinatal mortality is significantly high in the pups obtained from primiparous females than multiparous females of both the breeding methods. We compared the data of C57BL/6 mice with robust strain, B6D2F1. The overall results can be used as baseline data for our C57BL/6 and B6D2F1 strains by the ACTREC scientists as well as colony managers from other facilities.
Rectal stricture in a bonnet monkey (Macaca radiata) : A case report
Authors: S G Ramachandra , M Thirumala , V Ramesh 
Published: January 2025

A six-year-old female bonnet monkey was noticed with a bloated abdomen seven days after experimentation. A plain radiograph was taken to determine the cause of bloating. However, the plain radiograph did not reveal any significant changes except patches of gas in the abdomen. Hence, a contrast radiograph was done with a barium meal. A constriction was observed in the rectal region in contrast radiograph. An emergency laparotomy was done and stricture was surgically corrected by an end-to-end anastomosis.
Microbiological monitoring of Rodent pathogens using Multiplex method of PCR
Authors: Raj Mathan , Shinde Aarti , Ingle Arvind 
Published: January 2025

Microbiological quality control is undertaken to check the health status of laboratory animals. Pathogens infect the laboratory animals in various respiratory, digestive, central nervous systems, haematopoietic systems, dermal systems, etc. PCR detects pathogens with high sensitivity and specificity when compared to ELISA. Multiplex PCR can detect two or more pathogens at the same time. Multiplex PCR gives result based on the enzyme activity, bonding between the primer and DNA. In the present study, animal samples were analysed for the pathogen DNA at the molecular level to know the health status of laboratory animals. The respiratory pathogens Mycoplasma pulmonis and Sendai virus were detected simultaneously at 245, 340 bp, respectively. The digestive pathogens Salmonella typhimurium and Clostridium piliforme were detected simultaneously at 246, 196 bp, respectively. The animals were free from the specific respiratory and digestive pathogens. The multiplex PCR method is beneficial to detect the various pathogens in the laboratory rodents using the high throughput method.
Surveillance, integrity and metadata in the information age
Authors: Bennesved Peter 
Published: January 2025

In August 1994, the Prime Minister of Sweden, Carl Bildt, publicly announced the formation of The Information and Communication Technology Commission. This was an advisory commission, comprised of ministers in Bildt’s government cabinet as well as invited experts and entrepreneurs. The formation of the ICT Commission has been understood as a pivotal moment in Swedish policymaking within the ICT area. However, an underappreciated part of the ICT Commission’s history is that while it is generally acknowledged that the introduction of ICT in the 1990s was positively received by political elites and intellectuals, public scepticism toward ICT was a known problem and something the commission needed to address. Inspired by the concept of sociotechnical imaginaries (STIM), developed by STS scholars Jasanoff and Kim, the ICT Commission can be interpreted as a sort of state-financed “embedding activity” meant to overcome public scepticism of perceived risks, and to gather around a common vision of the future. Starting from a STIM perspective, the aim of this article is to deepen our understanding of this period in Swedish ICT policymaking, and its consequences. Studying the way the ICT Commission addressed topics of surveillance, personal integrity and metadata is important in that it provides a new and more complex perspective on the commission’s work and its public role. The article also connects the commission’s history to contemporary discussions about surveillance cultures and asks if the Commission – in a longer perspective – can be said to have fostered a positive attitude toward data sharing.
TỶ LỆ STREPTOCOCCUS NHÓM B DƯƠNG TÍNH TẠI MẪU DỊCH ÂM ĐẠO - TRỰC TRÀNG CỦA THAI PHỤ 36-38 TUẦN TẠI BỆNH VIỆN PHỤ SẢN QUỐC TẾ SÀI GÒN
Authors: Trần Thị Hai , Nguyễn Hữu Trung 
Published: January 2025

Mục tiêu: Xác định tỷ lệ GBS dương tính và các yếu tố liên quan tại mẫu dịch âm đạo – trực tràng ở thai phụ 36 – 38 tuần tại Bệnh viện Phụ sản Quốc tế Sài Gòn. Đối tượng và phương pháp nghiên cứu: Thiết kế nghiên cứu cắt ngang khảo sát 280 thai phụ có tuổi thai từ 36 – 38 tuần tại bệnh viện Phụ Sản Quốc Tế Sài Gòn, được thực hiện nuôi cấy định danh vi khuẩn Streptococcus nhóm B. Kết quả: Tỷ lệ nhiễm GBS âm đạo – trực tràng ở thai phụ 36 – 38 tuần tại Bệnh viện Phụ sản Quốc tế Sài Gòn là 12,3%. Các yếu tố liên quan đến tỷ lệ nhiễm GBS là tuổi của thai phụ (≥ 35) (OR 1,98, KTC 1,14 - 3,45), tiền căn viêm âm đạo (OR 2,98, KTC 1,09 – 8,14), triệu chứng lâm sàng viêm âm đạo (OR 3,09, KTC 1,41 – 6,76), thai phụ vệ sinh sau tiểu bằng nước (OR: 2,32; KTC: 1,07-4,99), giao hợp trong vòng 1 tháng trước XN (OR 2,79, KTC 1,14 – 6,83). Ampicillin, Penicillin, Cefepime, Ceftriaxone, Linezoid và Vancomycin nhạy 100%. Kháng sinh Tetracycline kháng 100%; Erythromycin, Clarithromycin và Clindamycin có tỷ lệ kháng lần lượt là 42,9%, 39,3% và 35,7%. Kết luận: Cần tăng cường tầm soát GBS ở những thai phụ có tuổi từ 35 trở lên. Tiền căn viêm âm đạo và triệu chứng lâm sàng viêm âm đạo là hai yếu tố nguy cơ làm gia tăng khả năng GBS dương tính ở thai phụ cần được quan tâm.
Pengembangan Media Pembelajaran Berdifferensiasi untuk Meningkatkan Pemahaman Konsep pada Materi Pecahan
Authors: Masdiana Masdiana , Ilyas Muhammad , Taufiq Taufiq 
Published: January 2025

Pemahaman konsep matematika salah satu kemampuan yang diperlukan dalam memahami konsep matematis serta menjelaskan keterkaitan antar konsep dan menggunakannya dalam menyelesaikan soal dan masalah. Media pembelajaran berdiferensiasi diperlukan agar siswa dapat memahami permasalahan pada soal matematika yang akan memudahkan siswa untuk memilih solusi penyelesaian yang tepat. Jenis penelitian ini adalah penelitian pengembangan atau research and development (RnD). Tujuan penelitian ini adalah untuk mengetahui proses dan hasil pengembangan media pembelajaran berdiferensiasi untuk meningkatkan kemampuan pemahaman konsep pada materi pecahan di UPT SD Negeri 129 Uraso yang valid, praktis, dan efektif. Penelitian ini dilaksanakan di Kelas V UPT SD Negeri 129 Uraso Kecamatan Mappedeceng Kabupaten Luwu Utara Semester Genap Tahun Akademik 2023/2024. Prosedur pengembangan pada penelitian ini menggunakan model pengembangan 4D yaitu define, design, development, dan disseminate. Instrumen Penelitian yang digunakan yaitu angket respon guru terhadap media pembelajaran, angket respon siswa, dan tes kemampuan pemahaman konsep siswa. Teknik analisis data dalam penelitian ini menggunakan teknik analisis deskriptif yang meliputi analisis kualitatif dan analisis kuantitatif yang bertujuan untuk mengetahui proses dan hasil pengembangan media pembelajaran berdiferensiasi yang valid, praktis, dan efektif. Hasil penelitian menunjukkan bahwa (1) hasil validasi ahli materi menilai media pembelajaran berdiferensiasi berada kategori sangat valid sedangkan hasil validasi ahli media menilai media pembelajaran berdiferensiasi berada pada kategori cukup valid, (2) Produk media pembelajaran berdiferensiasi mendapat tanggapan positif dan memenuhi kriteria praktis. Skor lembar observasi keterlaksanaan pembelajaran berada pada kategori sangat praktis, respon siswa berada pada kategori sangat praktis, dan respon guru berada pada kategori sangat praktis, (3) Produk media pembelajaran berdiferensiasi memenuhi kriteria efektif.
BCUB – a large-sample ungauged basin attribute dataset for British Columbia, Canada
Authors: Kovacek Daniel , Weijs Steven 
Published: January 2025

Abstract. The British Columbia Ungauged Basin (BCUB) dataset is an open-source, extensible dataset of attributes describing terrain, soil, land cover, and climate indices of over 1.2 million ungauged catchments in British Columbia, Canada, including trans-boundary regions. The attributes included in the dataset are similar to those found in the large-sample hydrology literature for their association with hydrological processes. The BCUB database is intended to support water resources research and practice, namely monitoring network analysis studies, or hydrological modelling where basin characterization is used for model calibration. The dataset and the complete workflow to collect and process input data, to derive stream networks, and to delineate sub-basins and extract attributes, are available under a Creative Commons BY 4.0 license. The DOI link for the BCUB dataset is https://doi.org/10.5683/SP3/JNKZVT (Kovacek and Weijs, 2023).
Quantifying uncertainties in satellite NO2 superobservations for data assimilation and model evaluation
Authors: Rijsdijk Pieter , Eskes Henk , Dingemans Arlene , Boersma K. Folkert , Sekiya Takashi , Miyazaki Kazuyuki , Houweling Sander 
Published: January 2025

Abstract. Satellite observations of tropospheric trace gases and aerosols are evolving rapidly. Recently launched instruments provide increasingly higher spatial resolutions, with footprint diameters in the range of 2–8 km and with daily global coverage for polar orbiting satellites or hourly observations from geostationary orbits. Often the modelling system has a lower spatial resolution than the satellites used, with a model grid size in the range of 10–100 km. When the resolution mismatch is not properly bridged, the final analysis based on the satellite data may be degraded. Superobservations are averages of individual observations matching the model's resolution and are functional to reduce the data load on the assimilation system. In this paper, we discuss the construction of superobservations, their kernels, and uncertainty estimates. The methodology is applied to nitrogen dioxide tropospheric column measurements of the TROPOspheric Monitoring Instrument (TROPOMI) instrument on the Sentinel-5P satellite. In particular, the construction of realistic uncertainties for the superobservations is non-trivial and crucial to obtaining close-to-optimal data assimilation results. We present a detailed methodology to account for the representation error when satellite observations are missing due to, e.g., cloudiness. Furthermore, we account for systematic errors in the retrievals leading to error correlations between nearby individual observations contributing to one superobservation. Correlation information is typically missing from the retrieval products, where an error estimate is provided for individual observations. The various contributions to the uncertainty are analysed from the spectral fitting and the estimate of the stratospheric contribution to the column and the air mass factor for which we find a typical correlation length of 32 km. The method is applied to TROPOMI data but can be generalized to other trace gases such as HCHO, CO, and SO2 and other instruments such as the Ozone Monitoring Instrument (OMI), the Geostationary Environment Monitoring Spectrometer (GEMS), and the Tropospheric Emissions: Monitoring of POllution (TEMPO) instrument. The superobservations and uncertainties are tested in the Multi-mOdel Multi-cOnstituent Chemical (MOMO-Chem) data assimilation ensemble Kalman filter system. These are shown to improve forecasts compared to thinning or compared to assuming fully correlated or uncorrelated uncertainties within the superobservation. The use of realistic superobservations within model comparisons and data assimilation in this way aids the quantification of air pollution distributions, emissions, and their impact on climate.
Voices of Indigenous Peoples on National Capital Relocation: Evidence from Indonesia
Authors: Suradi  , Koeswinarno  , M. Fakhruddin , Aswin Azwar , Silitonga Metha Claudia Agatha , Rahmawati Atik , Zakiyah  
Published: January 2025

IKN Nusantara, the new national capital of Indonesia, is being developed in an area inhabited by indigenous peoples. IKN development is characterized by a government-led top-down strategy implemented by the IKN Authority. Although the Indonesian government has recognized the existence of indigenous peoples in this area, the formal approach taken poses challenges for indigenous peoples to defend their land. Therefore, this study used the Free, Prior, and Informed Consent (FPIC) approach to investigate the voices of indigenous peoples who have lived in the IKN Nusantara area for generations. The results reveal that indigenous peoples affected by the IKN relocation do not have adequate access to information. Furthermore, consultation and consent procedures, as well as participation by indigenous peoples, are also lacking. Indigenous peoples have voiced their concerns about potential displacements resulting from urban development projects, which is akin to those faced by the Betawi community in Jakarta. This study highlights the need for more inclusive and transparent processes to ensure the rights of indigenous peoples are respected.
Technical Proficiency Analysis in Table Tennis: A Comparative Study Between Advanced and Intermediate Players
Authors: Aprial Benny , Setyawan Denny , Mocanu George Danut , Tarigan Candra Brema , Mustafa Ghulam , Aquino John Michael D. , Sarıtaş Bilal Azmi 
Published: January 2025

The purpose of the study. This study presents a comprehensive examination of technical proficiencies in table tennis, comparing advanced and intermediate players to identify key differentiating factors in performance.
Materials and methods. The research involved 40 participants (20 advanced players with 5+ years of competitive experience and 20 intermediate players with 2-4 years of experience) aged 18-25 years. Over a three-month period, participants underwent systematic evaluation of their technical skills, including basic stroke accuracy, biomechanical efficiency, and tactical adaptability. This empirical investigation was undertaken at seven Table Tennis clubs located within the confines of Medan city, Indonesia.
Results. Results revealed significant differences between skill levels in most measured parameters: advanced players demonstrated superior forehand drive accuracy (85.3% vs 67.1%, p<0.001), backhand drive accuracy (82.7% vs 63.4%, p<0.001), and rally consistency (28.4 vs 15.6 hits, p<0.001). Biomechanical analysis showed advanced players maintained more optimal arm angles (110.5° vs 95.8°) and faster bat swing speeds (17.8 m/s vs 12.4 m/s). Additionally, advanced players exhibited better tactical adaptation, with more service variations (6.8 vs 4.2 types) and faster reaction times (245ms vs 312ms).
Conclusions. The findings highlight that the progression from intermediate to advanced level requires improvements across multiple domains, including technical consistency, biomechanical efficiency, and tactical adaptability. This research provides valuable insights for developing targeted training programs and understanding the multifaceted nature of expertise in table tennis.
Transit-Oriented Development: Evaluating View Sheds and Public Spaces around Transit Nodes at Bittan Market and 10 No. Market, Bhopal
Authors:   Asst. Professor, School of Architecture, RGPV, Bhopal, Hanfi Faisal 
Published: January 2025

Walking distances conventionally define service areas for public transportation and urban growth boundaries for Transit-Oriented Development (TOD). Urban designers and planners typically draw rings and lines around transit stops and arrange transit-supportive land within these boundaries. However, this approach neglects the processes of creating public spaces in visual proximity to transit stops, which can have a significant impact on urban growth patterns and land usage. This paper proposes a methodology to analyse public spaces by considering how commercialization and public space patterns unfold through view sheds around transit stops. The results reveal amoebic TOD patterns of public spaces that differ from conventional walking radii. This alternative view shed approach offers a more nuanced and dynamic tool for designing TODs and fostering effective urban spaces around transit hubs.

Key Words: Walking distances, public transportation, Transit-Oriented Development (TOD), View sheds, Urban growth patterns.
Real-Time Retrieval-Augmented Generation in Healthcare Using LLMs for Audio-based Querying and Retrieval
Authors: Arora1 Kartik 
Published: January 2025

Healthcare information systems are critical for efficient decision-making and patient care. This paper presents a real-time audio-query-driven retrieval-augmented generation (RAG) system using a large language model (LLM) that interfaces with a healthcare database and a vector database. The system allows users to make real-time audio queries, retrieves the necessary information using LLM tools, and delivers audio responses. Comparative performance evaluations between traditional healthcare information systems and our proposed LLM-based approach show significant improvements in query throughput, cost-efficiency, and response time.

Key Words: RAG, LLM, healthcare database, real-time audio input, vector database, AI in healthcare
สภาพปัญหาในการบริหารงบประมาณขององค์กรปกครองส่วนท้องถิ่นในพื้นที่จังหวัดสงขลา
Authors: กาญจนมุกดา วสันต์ , รัตนสุวรรณ ยงยุทธ , นวลละออง พัชรทิตา , อิสรรักษ์ ศรัณยา , หิตะพงศ์ โชติญาณ์ , บัวศรี ฉันทิชา , จันทร์ประสิทธิ์ เสาวลักษณ์ , นวลประดิษฐ์ ศิรดา , ขาวขจร วิทยา 
Published: January 2025

งานวิจัยนี้เป็นการศึกษาสภาพปัญหา เปรียบเทียบสภาพปัญหาจำแนกตามปัจจัยส่วนบุคคล ในการบริหารงบประมาณขององค์กรปกครองส่วนท้องถิ่นในพื้นที่จังหวัดสงขลา จากกลุ่มตัวอย่างผู้ปฎิบัติงานที่เกี่ยวข้องกับกระบวนการงบประมาณขององค์กรปกครองส่วนท้องถิ่นที่มีสถานะเป็นองค์การบริหารส่วนจังหวัด และเทศบาลในพื้นที่จังหวัดสงขลา ประกอบด้วยคณะผู้บริหาร ปลัด สมาชิกสภาท้องถิ่น และผู้ปฏิบัติงาน จำนวน 106 คน สถิติที่ใช้ ได้แก่ ค่าร้อยละ ค่าเฉลี่ยเลขคณิต ค่าส่วนเบี่ยงเบนมาตรฐาน ค่าทดสอบที และค่าทดสอบเอฟ
ผลการวิจัย พบว่า การบริหารงานงบประมาณองค์กรปกครองส่วนท้องถิ่นในพื้นที่จังหวัดสงขลา ในภาพรวมมีปัญหาการบริหารงบประมาณในระดับปานกลาง ผลการเปรียบเทียบสภาพปัญหาในการบริหารงบประมาณขององค์กรปกครองส่วนท้องถิ่นในพื้นที่จังหวัดสงขลาจำแนกตามเพศ วุฒิการศึกษา ประสบการณ์ทำงาน และตำแหน่ง โดยภาพรวมและรายด้านพบว่า ไม่แตกต่างกัน ทั้งนี้ควรมีการส่งเสริมให้บุคลากรภายในและภายนอกองค์การบริหารส่วนท้องถิ่น รวมทั้งสมาชิกสภาท้องถิ่น มีความรู้ความเข้าใจในระเบียบ กฎหมาย หนังสือสั่งการ โดยจัดทำเป็นคู่มือ มีการซักซ้อมแนวทางปฏิบัติงานขององค์กรปกครองส่วนท้องถิ่น และนำไปปฏิบัติได้อย่างถูกต้อง แม่นยำ การนำนโยบายสู่การปฏิบัติ องค์กรปกครองส่วนท้องถิ่นควรนำนโยบายทุกระดับมาเป็นทิศทาง แนวทาง การจัดทำงบประมาณขององค์กรปกครองส่วนท้องถิ่นให้ครอบคลุมภารกิจและอำนาจหน้าที่ รวมทั้งให้มีกลไกในการกำกับ ติดตาม ตรวจสอบการบริหารงบประมาณที่มีประสิทธิภาพ