bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green investment, financial efficiency, and sustainable development: Dual perspectives of enterprise cost and government guidance
Authors: Zhao Lijuan , Zhu Tianqi , Shi Junhong , Kang Xiaohong , Chen Haonan 
Published: December 2023

Abstract

The present study highlights the importance of green investment in achieving the goal of sustainability. Several studies have been conducted to explore the factors affecting sustainable development. However, the literature on the importance of green investment in affecting sustainable development from the perspective of enterprise cost and government guidance is scant, and the moderating effect of financial efficiency is also ignored. Thus, this study is based on the social and economic data of 31 provinces in China from 2009 to 2021, the study evaluates the level of sustainable development of each province and combines green investment, financial efficiency, and sustainable development into the research framework from the dual perspectives of government and enterprises to explore the relationship among the three, which can make sustainable development efficient and balanced. The results show the following: (1) Government green investment has a “U-shaped” spillover effect on sustainable development; (2) Corporate green investment has an inverted U-shaped nonlinear effect on sustainable development; and (3) Under the adjustment of financial efficiency, government green investment has a “two-stage” promoting effect on sustainable development, and corporate green investment has an inverted U-shaped nonlinear effect on sustainable development. Based on these findings, it is recommended that increase the amount of government green investment, ensure the sustainable amount of corporate green investment, and promote coordinated financial and economic development.
Spontaneous symmetry breaking of non-Hermitian coupled nano-cavities
Authors: Cheah Kok Wai , Mai Jianming , Huang Xiansheng , Guo Xu , Fan Haihua 
Published: December 2023

Abstract

Experimental spontaneous symmetry breaking results are observed from a non-Hermitian coupled cavities device. In the experiment, when horizontally polarised incident light exceeds \(8 \text{m}\text{W}\), the symmetry-breaking threshold, vertically polarised transmitted light is also detected in addition to the horizontally polarised transmitted light, i.e. spontaneous symmetry breakdown of polarisation occurred. To elucidate more details on physics of the symmetry breakdown, theoretical modelling is also performed. Using nonlinear coupled mode theory (NCMT) equations, a power-related random splitting of the two handedness polarised lights is established, namely, right- or left- handed circular polarisation (RCP and LCP), at the resonance peak. In the numerical simulations, when incident power is above the symmetry-breaking threshold, the device will exhibit spontaneous symmetry breaking characteristics viz. appearance of an additional polarisation component in the output field. The additional polarisation direction is orthogonal to the linearly polarised incident light. These findings offer further understanding spontaneous symmetry breaking in non-Hermitian systems, and also demonstrate the potential applications of the proposed device in optical signal processing.
Epidemiological Characteristics of Influenza Outbreaks in Schools in Jiangsu Province, China, 2020- 2023 Post-COVID-19 Pandemic
Authors: Peng Jia-Le , Xu Ke , Huang Hao-Di , Bao Chang-Jun , Dai Qi-Gang 
Published: December 2023

Abstract

Background
To understand the epidemic characteristics and influencing factors of school influenza outbreaks after the COVID-19 pandemic in Jiangsu Province, China from 2020 to 2023, and provide scientific basis for the prevention and control of school influenza.
Methods
We collected data on influenza-like illness(ILI) outbreaks reported in the Chinese Influenza Surveillance Information System and monitoring data from national-level influenza surveillance sentinel hospitals. Descriptive statistical analysis was conducted on the temporal distribution, school type distribution, virus strain distribution, and epidemic scale of school influenza outbreaks.
Results
From 2020 to 2023, a total of 833 influenza outbreaks occurred in schools in Jiangsu Province. The most reported school type was primary schools, catering to the age group of 6–12 years, accounting for 70.11% of the total reports. This study included three complete influenza seasons, with the majority of large-scale outbreaks caused by A(H1N1) and A(H3N2), accounting for 9.09% of the total outbreaks caused by them,. Outbreaks occurring in the early stages of the B(Victoria) and A(H1N1) influenza peaks accounted for 86.31% and 97.30%, respectively. The current surveillance data has not yet observed a concurrent outbreak of influenza and COVID-19.
Conclusion
Primary and secondary schools are high-risk locations for influenza outbreaks. The higher environmental adaptability of A(H3N2) makes it more prone to co-circulate with other subtypes/lineages, especially during concurrent circulation with A(H1N1), increasing the likelihood of large-scale outbreaks. In contrast, outbreaks caused by B (Victoria) show a higher frequency and relatively smaller scale compared to those triggered by influenza A. School influenza outbreaks are more likely to occur in the early stages of seasonal epidemics, indirectly indicating that the occurrence of influenza in schools plays a crucial role in driving the onset and development of influenza within the broader community/population. In the post-COVID-19 pandemic period, it is essential to prioritize influenza monitoring and take measures early to reduce the occurrence of outbreaks.
Haptoglobin attenuates cerebrospinal fluid hemoglobin-induced neurological deterioration in sheep
Authors: R.	Thomson Bart , Nina	Schwendinger  , Katrin	Beckmann  , Thomas	Gentinetta  , Daniel	Couto  , Sandra	Wymann  , Valérie	Verdon  , M.	Buzzi Raphael , Kevin	Akeret  , W.	Kronen Peter , Eva	Weinberger  , Ulrike	Held  , Frauke	Seehusen  , Henning	Richter  , J.	Schaer Dominik , Michael	Hugelshofer  
Published: December 2023

Abstract

Background
Secondary brain injury (SBI) occurs with a lag of several days post-bleeding in patients with aneurysmal subarachnoid hemorrhage (aSAH) and is a strong contributor to mortality and long-term morbidity. aSAH-SBI coincides with cell-free hemoglobin (Hb) release into the cerebrospinal fluid. This temporal association and convincing pathophysiological concepts suggest that CSF-Hb could be a targetable trigger of SBI. However, sparse experimental evidence for Hb’s neurotoxicity in vivo defines a significant research gap for clinical translation.
Methods
We modeled the CSF-Hb exposure observed in aSAH patients in conscious sheep, which allowed us to assess neurological functions. Twelve animals were randomly assigned for three-day bi-daily intracerebroventricular (ICV) injections of either Hb or Hb combined with the high-affinity Hb scavenger protein haptoglobin (Hb-Hp, CSL888).
Results
Repeated CSF sampling confirmed clinically relevant CSF-Hb concentrations. This prolonged CSF-Hb exposure over three days resulted in disturbed movement activity, reduced food intake, and impaired observational neuroscores. The Hb-induced neurotoxic effects were significantly attenuated when Hb was administered with equimolar haptoglobin. Preterminal magnetic resonance imaging (MRI) showed no CSF-Hb-specific structural brain alterations. In both groups, histology demonstrated an inflammatory response and revealed enhanced perivascular histiocytic infiltrates in the Hb-Hp group, indicative of adaptive mechanisms. Heme exposure in CSF and iron deposition in the brain were comparable, suggesting comparable clearance efficiency of Hb and Hb-haptoglobin complexes from the intracranial compartment.
Conclusion
We identified a neurological phenotype of CSF-Hb toxicity in conscious sheep, which is rather due to neurovascular dysfunction than structural brain injury. Haptoglobin was effective at attenuating CSF-Hb-induced neurological deterioration, supporting its therapeutic potential.
Genome-wide methylation profiling reveals extracellular vesicle DNA as an ex vivo surrogate of cancer cell-derived DNA
Authors: Kim Kyung-A , Kim Sunmin , Wortzel Inbal , Kim Tae-Min , Kim Han Sang 
Published: December 2023

Abstract

Extracellular vesicle-derived DNA (evDNA) encapsulates the complete genome and mutational status of cells; however, whether cancer cell-derived evDNA mirrors the epigenetic features of parental genomic DNA remains uncertain. This study aimed to assess and compare the DNA methylation patterns of evDNA from cancer cell lines and primary cancer tissues with those of the nuclear genomic DNA. We isolated evDNA secreted by two cancer cell lines (HCT116 and MDA-MB-231) from various subcellular compartments, including the nucleus and cytoplasm. Additionally, we obtained evDNA and nuclear DNA (nDNA) from the primary cancer tissues of two patients. We conducted a comprehensive genome-wide DNA methylation analysis using the Infinium Methylation EPIC BeadChip, examining > 850,000 CpG sites. Remarkable similarities were observed between the methylation patterns of evDNA and nDNA in cancer cell lines. This concordance extended to clinical cancer tissue samples, showcasing the potential utility of evDNA methylation patterns in deducing cellular origin within heterogeneous populations through methylation-based deconvolution. The observed concordance underscores the utility of evDNA as a noninvasive surrogate marker for discerning tissue origin, particularly in cancerous tissues. This finding enhances our understanding of cellular origins and would help in developing innovative diagnostic and therapeutic strategies for cancer.
An advanced approach for predicting Selective Sweep in the genomic regions using machine learning techniques
Authors: Sarkar Abhik , Mishra Dwijesh Chandra , Sinha Dipro , Chaturvedi Krishna Kumar , Lal Shashi Bhushan , Kumar Sanjeev , Jha Girish Kumar , Budhlakoti Neeraj 
Published: December 2023

Abstract

Selective Sweep is an important phenomenon in the aspect of natural selection. It plays significant role in adaptability as well as survival of species, crop varieties etc. Various existing approaches for selective sweep analysis are mostly built on traditional rule base approach which lack the advanced approaches such as machine learning and deep learning and often result in poor prediction accuracy. In this study a new method or model for the prediction of selective sweep has been presented. This method has been initiated with simulation, preceded through feature extraction and selection and finally fed to different machine learning algorithms. Here eight different machine learning based methods have been implemented − 1) Support Vector Machine (SVM), 2) Regression Tree, 3) Random Forest, 4) Naive Bayes, 5) Multiple logistic regression, 6) K-Nearest Neighbor (KNN), 7) Gradient boosting and 8) Artificial Neural Network (ANN) and results of their comparative evaluations are presented. It has been observed that random forest model outperformed to its counterparts in terms of evaluation matrices with an AUC score of 0.8448 as well as 1st rank in TOPSIS analysis. Further, a robust model for selective sweep prediction based upon random forest has been developed. Model developed in the current study has outperformed to other existing approaches for prediction and analysis of selective sweep. This new approach for selective sweep analysis is excellent in its accuracy as well as reliability.
Genetic diversity of the endemic Durian Kura-kura (Durio testudinarius Becc.) using morphology and SSR markers
Authors: Magandhi Mahat , ' Sobir , Matra Deden Derajat , Wahyu Yudiwanti , ' Sudarmono 
Published: December 2023

Abstract

Durio testudinarius, commonly known as Durian kura-kura, represents a wild durian species within the Malvaceae family, exclusive to Borneo. Understanding the genetic diversity of this species is crucial for effective genetic resource management. In this study, we conducted a comprehensive analysis of the genetic diversity of D. testudinarius across five populations in West Kalimantan, namely Sambas, Landak, Sanggau, Sekadau, and Ketapang. Our approach integrated both morphological and molecular markers, incorporating 18 vegetative morphological characters and 20 microsatellite loci. Morphological analysis revealed variations among D. testudinarius accessions, encompassing traits such as tree height, leaf blade dimensions, trunk surface features, and various leaf characteristics. On the molecular level, all 20 microsatellite loci exhibited polymorphism, with allele frequencies ranging from 0.025 to 0.975. These loci collectively comprised 84 alleles across 20 accessions, averaging 4.2 alleles per locus. The observed heterozygosity and expected heterozygosity values were 0.263 and 0.268, respectively. Polymorphic information content values ranged from 0.049 to 0.869, with an average of 0.454. Ward-based cluster analysis using morphological, SSR, and combined markers generated a dendrogram, indicating that the Landak, Sekadau, and Sanggau populations tended to cluster together, while the Sambas and Ketapang populations exhibited a tendency to form distinct groups. This study provides valuable insights into the genetic diversity of Durio testudinarius, facilitating informed conservation and management strategies for this unique wild durian species in Borneo.
Identification of key genes CCL5, PLG, LOX and C3 in clear cell renal cell carcinoma through integrated bioinformatics analysis
Authors: Xie Zhenwei , 邓 玮明 , Zhao Jinli , Chen Libo , Li Mingyong , Deng Weiming 
Published: December 2023

Abstract
Background
Clear Cell Renal Cell Carcinoma (ccRCC) is a malignant tumor with high mortality and recurrence rates and the molecular mechanism of ccRCC genesis reminds unclear. In this study, we screen out several key genes associated with the prognosis of ccRCC by using integrated bioinformatics.
Methods
Two ccRCC expression profiles were downloaded from GEO and one dataset was gained from TCGA. RRA method was used to analyze the three datasets to gain integrated differentially expressed gene (DEGs) by comparing ccRCC with normal tissues. The GO functional annotation and KEGG pathway analysis were performed to analyze the potential functions of these DEGs. The STRING and Cytoscape were used to construct PPI network and module analyses to screen the hub genes. The expression of hub genes was analyzed using GEPIA, followed by RT-qPCR and IHC to validate the expression of hub genes between ccRCC and adjacent normal tissues. Finally, the prognostic value of these hub genes for ccRCC patients were identified by K-M plotter.
Result
125 DEGs were identified by using the limma package and RRA method, which include 62 up-expressed genes and 63 down-expressed genes.GO analysis showed that the up-expressed genes were primarily enriched in signal transduction, immune response and cell-cell signaling. The down-expressed genes were primarily enriched in ion transmembrane transport, excretion and transport. The top five enriched pathways gained from the KEGG pathway analysis were complement and coagulation cascades, aldosterone-regulated sodium reabsorption, collecting duct acid secretion, PPAR signaling pathway and prion diseases. K-M plotter and GEPIA database were utilized to make clear that CCL5, LOX and C3 are not only up-expressed in ccRCC, but also had a connection with the poor prognosis of ccRCC. PLG is down-expressed in ccRCC, which associated with the better prognosis of ccRCC. RT-qPCR and IHC assays also confirmed the differential expression of these four hub genes in paired ccRCC and adjacent normal tissues.
Conclusion
These findings manifest that CCL5, LOX, C3 and PLG may play key roles in the progression and prognosis of ccRCC, which will be helpful for further studies to find the potential therapeutic targets and underlying mechanisms of ccRCC.
Interhospital Transfer Versus Other Modes of Admission of Patients With Covid-19 at the University Hospital Andohatapenaka: “a Retrospective Cohort Study”
Authors: RAKOTOARISOA Josoa ANDRIANIAINA , RAKOTOMAVO Falihery Albertin , RAKOTONAIVO Andoniaina , RAHARIMAHENINA Nirina Andrianome , RABENJARISON Francklin , RAVELOSON Nasolotsiry Enintsoa 
Published: December 2023

Abstract
Background
The COVID-19 pandemic did not spare Madagascar, and we observed that a significant number of inpatients who were transferred passed away. The aim of this study was to investigate how admission by transfer influences the outcome of COVID-19 patients and to identify factors associated with mortality in patients admitted through interhospital transfer.
Methods
This is a retrospective cohort study conducted at the Andohatapenaka University Hospital over a three-month period from December 2021 to February 2022. The first group included in the study population was composed of patients from interhospital transfers (n = 54). The second group consisted of patients admitted by other admission methods (n = 127). The chi-square test was used to check the validity of the relative risk (RR), and the significance threshold was set by a p value < 0.05.
Results
After analysis, it was found that the mode of admission interhospital transfer was associated with patient mortality (RR = 1.47 [1.06–2.04]). Additionally, we identified several factors associated with mortality in COVID-19 patients, including a history of diabetes (RR = 1.58[1.02–2.43]), a respiratory rate of more than 30 cycles/min (RR = 1.58[1.02–2.45]), SpO2 less than or equal to 88% (RR = 2.45[1.21–4.92]), Glasgow score between 9 and 12 (RR = 2.09[1.55–2.83]), critical form (RR = 2.41 [1.68–3.47]), lung involvement greater than 50% (RR = 1.99 [1.05–3.74]), presence of complications (RR = 1.90 [1.24–2.93]), complications by myocardial infarction (RR = 2.00 [1.50–2.65]), and use of noninvasive ventilation (RR = 2.00 [1.50–2.65]). Therefore, admission through interhospital transfer influenced the unfavorable patient outcome.
Conclusion
Our study shows that admission by transfer is associated with mortality in COVID-19 patients. We recommend that healthcare professionals exercise vigilance in managing these patients to improve outcomes.
TGFβ signaling pathway is altered by HLA-B27 expression, resulting in pathogenic consequences relevant for spondyloarthritis.
Authors: Lauraine Marc , De Tilques Maxence De Taffin , Melamed-Kadosh Dganit , Cherqaoui Bilade , Rincheval Vincent , Prevost Erwann , Rincheval-Arnold Aurore , Cela Eneida , Admon Arie , Guénal Isabelle , Araujo Luiza , Breban Maxime 
Published: December 2023

Abstract

Background
Association of HLA-B27 with spondyloarthritis (SpA) has been known for 50 years, but still remains unexplained. We recently showed that HLA-B27expressed in wing imaginal disc from HLA-B27/human-β2 microglobulin (hβ2m) transgenic Drosophila deregulated bone morphogenetic protein (BMP) pathway by interacting physically with type I BMP receptor (BMPR1) Saxophone (Sax), leading to crossveinless phenotype.
Methods
Genetic interaction was studied between activin/transforming growth factor β (TGFb) pathway and HLA-B27/hβ2m in transgenic Drosophila wings. The HLA-B27-bound peptidome was characterized in wing imaginal discs. In mesenteric lymph node (mLN) T cells from HLA-B27/hβ2m rat (B27 rat), physical interaction between HLA-B27 and activin receptor-like kinase-2 (ALK2), ALK3 and ALK5 BMPR1s, phosphorylation of small mothers against decapentaplegic (SMADs) and proteins of the non-canonical BMP/TGFb pathways induced by its ligands, and the transcript level of target genes of the TGFb pathway, were evaluated.
Results
In HLA-B27/hβ2m transgenic Drosophila, inappropriate signalling through the activin/TGFβ pathway, involving Baboon (Babo), the type I activin/TGFβ receptor, contributed to the crossveinless phenotype, in addition to deregulated BMP pathway. We identified peptides bound to HLA-B27 with the canonical binding motif in HLA-B27/hβ2m transgenic Drosophila wing imaginal disc. We demonstrated specific physical interaction, between HLA-B27/hb2m and mammalian orthologs of Sax and Babo, i.e. ALK2 and ALK5 (i.e. TGFb receptor I), in the mLN cells from B27 rat. The magnitude of phosphorylation of SMAD2/3 in response to TGFβ1 was increased in T cells from B27 rats, showing evidence for deregulated TGFb pathway. Accordingly, expression of several target genes of the pathway was increased in T cells from B27 rats, in basal conditions and/or after TGFb exposure, including Foxp3, Rorc, Runx1 and Maf. Interestingly, Tgfb1 expression was reduced in naïve T cells from B27 rats, even premorbid, an observation consistent with a pro-inflammatory pattern.
Conclusions
This study shows that HLA-B27 alters the TGFβ pathways in Drosophila and B27 rat. Given the importance of this pathway in CD4 + T cells differentiation and regulation, its disturbance could contribute to the abnormal expansion of pro-inflammatory T helper 17 cells and altered regulatory T cell phenotype observed in B27 rats.
Naming racism as a root cause of inequities in palliative care research: A scoping review
Authors: Algu Kavita , Wales Joshua , Anderson Michael , Omilabu Mariam , Briggs Thandi , Kurahashi Allison M. 
Published: December 2023

Abstract

Background Research is a core component in palliative care development and racial and ethnic inequities in palliative care are well-established. Examining this topic in an evidence informed manner, including the recognition of established root causes such as systemic racism, will allow researchers to substantively inform meaningful actions and policy changes to ultimately decrease these differences. Objective To summarize how peer reviewed literature has investigated racial and ethnic differences in palliative care over the last 13 years, exploring to what extent established root causes such as systemic and interpersonal racism are considered when contextualizing findings. Methods We searched bibliographic databases for primary, peer reviewed studies globally, in all languages, that collected race or ethnicity variables in a palliative care context (January 1, 2011 to October 17, 2023). We recorded study characteristics and categorized citations based on their research intention (if race or ethnicity were named in the study purpose), and the interpretation of findings (i.e. if systemic or interpersonal factors, including racism, were discussed when contextualizing the study results). Results Of 181 included studies, most were from the United States (88.95%) and purposefully investigated race or ethnicity (71.27%). Systemic or interpersonal factors were more often alluded to (41.44%) than explicitly named (20.44%). Racism was specifically mentioned in 7.18% of publications. There appears to be a trend toward explicitly naming racism from 2021–2023, with 36% of studies published in 2023 using the key word racis*. Conclusion Researchers hold power in shaping the trajectory of health disparities research regarding racial and ethnic disparities in palliative care, and informing subsequent actions and policy changes aimed to reduce these inequities. It is imperative that researchers use thoughtful methods in their studies, including purposeful research framing, questions and analysis; and that researchers ground these components in an evidence-based understanding of the root causes that drive these inequities, including the explicit naming of systemic and interpersonal racism.