bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial Cornea Substitute Based on Hydrogel Skeletons with Natural Stromal Hierarchical Structure and Extracellular Matrix for Sutureless Transplantation
Authors: Zhao Long , Shi Zhen , Zhang Xiaoyu , Wang Jingting , Yang Shang , Wang Fuyan , Li Tan , Zhou Qingjun , Wang Ting , Shi Weiyun 
Published: January 2025

AbstractCorneal substitutes with structural and compositional characteristics resembling those of natural corneas have attracted considerable attention. However, biomimicking the complex hierarchical organization of corneal stroma is challenging. In this study, humanized corneal stroma‐like adhesive patches (HCSPs) are prepared through a multi‐step process. First, polyethylene glycol diacrylate is cast and cured within decellularized porcine cornea (DPC) templates. The DPCs are then enzymatically digested to obtain hydrogel skeletons, which are finally integrated with human corneal extracellular matrix and methacrylate gelatin. HCSPs replicate the ultrastructure, protein components, and optical properties of human corneas and exhibit improved anti‐swelling and anti‐degradation capabilities compared with conventional DPCs and recombinant human collagen patches. HCSPs can deliver methacrylate gelatin at the ocular surface temperature (37 °C) and achieve stable adhesion to the corneal stroma upon 405 nm light irradiation. Furthermore, HCSPs promote the survival and migration of corneal epithelial and stromal cells while preserving their phenotypes. In rabbit models of lamellar keratoplasty and microperforation repair, HCSPs accelerate epithelial healing, minimize suture‐associated complications, and maintain structural stability. These findings suggest that HCSPs are promising donor corneal substitutes for clinical applications.
Flexible Synaptic Memristors With Controlled Rigidity in Zirconium‐Oxo Clusters for High‐Precision Neuromorphic Computing
Authors: Cho Jae‐Hyeok , Chun Suk Yeop , Kim Ga Hye , Sriboriboon Panithan , Han Sanghee , Shin Seung Beom , Kim Jeehoon , Nam San , Kim Yunseok , Kim Yong‐Hoon , Yoon Jung Ho , Kim Myung‐Gil 
Published: January 2025

AbstractFlexible memristors are promising candidates for multifunctional neuromorphic computing applications, overcoming the limitations of conventional computing devices. However, unpredictable switching behavior and poor mechanical stability in conventional memristors present significant challenges to achieving device reliability. Here, a reliable and flexible memristor using zirconium‐oxo cluster (Zr6O4OH4(OMc)12) as the resistive switching layer is demonstrated. The optimization of the structural rigidity of the hybrid oxo‐cluster network by thermal polymerization allows the precise formation of dispersed conductive cluster networks, enhancing the repeatability of the resistive switching with mechanical flexibility. The optimized memristor exhibits endurance of ∼104 cycles and stable memory retention performance up to 104 s, maintaining a high ION/IOFF ratio of 104 under a bending radius of 2.5 mm. Moreover, the device achieves a pattern recognition accuracy of 97.44%, enabled by highly symmetric analog switching with multilevel conductance states. These results highlight that hybrid metal‐oxo clusters can provide novel material design principles for flexible and reliable neuromorphic applications, contributing to the development of artificial neural networks.
The Innate Immune Sensor Zbp1 Mediates Central Nervous System Inflammation Induced by Angiostrongylus Cantonensis by Promoting Macrophage Inflammatory Phenotypes
Authors: Zhou Hongli , Zhou Minyu , Liao XiPing , Zhang Liangyu , Wei Hang , Lu Yuting , Zhang Yiqing , Huang Hui , Hu Yue , Chen Tao , Lv Zhiyue 
Published: January 2025

AbstractAngiostrongylus cantonensis (AC) is the leading cause of eosinophilic meningoencephalitis worldwide. The neuroimmune interactions between peripheral and central immune systems in angiostrongyliasis remain unclear. In this study, significant infiltration of eosinophils, myeloid cells, macrophages, neutrophils, and Ly6C monocytes is observed in the brains of AC‐infected mice, with macrophages being the most abundant. RNA‐seq and SMART‐seq analysis of pattern recognition receptor (PRR) and DNA sensor gene sets revealed a marked increase in Z‐DNA binding protein 1 (Zbp1) expression in infected mice. Confocal microscopy, RT‐qPCR, western blotting, and immunohistochemistry further confirmed that Zbp1 is specifically upregulated in macrophages and microglia. Using Zbp1‐knockout mice and flow cytometry, it is found that knockout of Zbp1 enhanced lymphocyte infiltration and natural killer cell cytotoxicity, modulating the immune microenvironment in the central nervous system (CNS) during AC infection. Mechanistically, it is revealed that in macrophage Zbp1 directly binds to receptor‐interacting protein 3 (RIP3) to promote its phosphorylation, subsequently facilitating the phosphorylation of mixed lineage kinase domain‐like protein (Mlkl). The activated Zbp1‐pRIP3‐pMlkl axis leads to necroptosis and upregulates pro‐inflammatory cytokines including TNF‐α, IL‐1α, CXCL9, CXCL10 in macrophages, which recruits and activates immune cells. These findings offer new insights into the pathogenic mechanisms of angiostrongyliasis and suggest potential therapeutic strategies.
Advantages of subtotal gastrectomy for upper third gastric cancer: A systematic review and meta‐analysis
Authors: Miki Yuichiro , Bito Tsubasa , Koterazawa Yasufumi , Kanaji Shingo , Shinohara Hisashi 
Published: January 2025

AbstractDistal gastrectomy with very small remnant stomach (subtotal gastrectomy, STG) is sometimes performed for upper third gastric cancer, instead of total gastrectomy (TG). However, the advantages of STG over TG remain fully unknown, in terms of survival outcomes, complication rates, nutritional status, and quality of life. Therefore, a systematic review was performed in accordance with the preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) extension statement for reporting systematic reviews. Meta‐analyses were performed regarding operation time, complication rates, body weight changes, and reflux esophagitis by using R software. Operation time was significantly shorter in STG than in TG (Mean difference: −20.79 [95% CI: −35.59 to −5.99]). We cannot conclude whether STG is better than TG in terms of survival because of inconsistency among reports. The total complication rates (≧ grade2 and 3) are significantly lower in STG than TG. Reflux esophagitis was lower in STG than in TG (0% vs. 7.7%), although the difference was not significant probably because of small sample size. Body weight change compared with preoperative value was significantly lower after STG than TG both at 6 and 12 months after the operation (6 months: mean difference −5.31% [95% CI: −6.95 to −3.67], 12 months: mean difference −6.13 [95% CI: −8.20 to −4.06]). We revealed that STG has its advantage on shorter operation time, less complication rates, and lower body weight loss by meta‐analysis. Although there are some controversies, STG can be an ideal option for patients with gastric cancer of upper third stomach.
Clinical significance of preoperative Glasgow prognostic score in patients with colorectal cancer and synchronous peritoneal metastases
Authors: Fujimoto Kosuke , Koyama Fumikazu , Kobayashi Hirotoshi , Kotake Kenjiro , Kawasaki Masayasu , Kanemitsu Yukihide , Kinugasa Yusuke , Ueno Hideki , Maeda Kotaro , Suto Takeshi , Itabashi Michio , Funahashi Kimihiko , Ozawa Heita , Noura Shingo , Ishida Hideyuki , Ohue Masayuki , Kiyomatsu Tomomichi , Ishihara Soichiro , Koda Keiji , Baba Hideo , Kawada Kenji , Hashiguchi Yojiro , Goi Takanori , Toiyama Yuji , Tomita Naohiro , Sunami Eiji , Fujita Fumihiko , Watanabe Jun , Hakamada Kenichi , Nakayama Goro , Sugihara Kenichi , Ajioka Yoichi 
Published: January 2025

AbstractBackgroundChemotherapy is the typical choice for treating colorectal cancer with synchronous peritoneal metastases. Nonetheless, surgical resection may be chosen if the metastases are resectable. Unfortunately, there is no reliable preoperative or intraoperative prognostic indicator. This study aimed to determine the prognostic significance of the preoperative Glasgow prognostic score (GPS) in colorectal cancer patients with synchronous peritoneal metastases.MethodsWe conducted a prospective study on 143 patients with colorectal cancer and concurrent peritoneal metastases. Our analysis included prognostic factors, such as the GPS, using data from the institutional observational study by the Japanese Society for Cancer of the Colon and Rectum.ResultsThe 3‐year survival rates for the GPS0 or 1 and GPS2 groups were 32.7% and 14.3%, respectively, with a significantly worse prognosis in the GPS2 group (p = 0.003). Multivariate analysis identified GPS2 (p = 0.006) and the peritoneal cancer index (PCI) (p = 0.029) or the Japanese surgical peritoneal metastasis grade (p = 0.009) as independent poor prognostic factors. Additionally, the GPS0 or 1 group with total resection of peritoneal metastases had a significantly better prognosis than the non‐resection group (p < 0.001); however, there was no difference between the GPS2 group with total peritoneal resection and the non‐resection group (p = 0.713).ConclusionsPreoperative GPS2 is an independent poor prognostic factor in patients with colorectal cancer and synchronous peritoneal metastases, and surgical resection does not improve prognosis in patients with GPS2. Preoperative GPSs may be used as indicators for surgical resection of synchronous peritoneal metastases.
Computer‐aided ionic liquids design and molecular insight for chloromethane dehydration
Authors: Zhao Fei , Cheng Yongqiang , Zhu Ruisong , Liu Qinghua , Liu Shuqing , Song Minghao , Lv Qingzhi , Jiang Bin , Lei Zhigang 
Published: January 2025

AbstractIn the process of butyl rubber production, excessive water content in chloromethane has a significant impact on the conversion and molecular weight distribution of the products. In this work, a computer‐aided ionic liquids design method was used for solvent design in the chloromethane dehydration process. The generate‐and‐test method was utilized to solve the mixed‐integer nonlinear programming problem formed in the design process. A multilayer perceptron was trained to predict the surface tension of ionic liquids. Quantum chemical calculations and molecular dynamics simulations were employed to test the dehydration performance of several designed ionic liquids. A candidate was selected for experimental synthesis and characterization. The impact of different ionic liquids on the vapor–liquid equilibrium of water was measured to confirm the feasibility, which indicates that the designed ILs exhibit a better dehydration potential compared to the basement solvent, 1‐Ethyl‐3‐methylimidazolium tetrafluoroborate. Anions play a leading role in the dehydration process.
Polygenic risk discriminates Lewy body dementia from Alzheimer's disease
Authors: McKeever Anna , Swann Peter , Malpetti Maura , Donaghy Paul C. , Thomas Alan , Mak Elijah , Carter Stephen F. , Tan Jerry H. K. , Hong Young T. , Fryer Tim D. , Heslegrave Amanda , Zetterberg Henrik , Su Li , Chouliaras Leonidas , Rowe James B. , O'Brien John T. 
Published: January 2025

AbstractINTRODUCTIONLewy body dementia (LBD) shares genetic risk factors with Alzheimer's disease (AD), including apolipoprotein E (APOE), but is distinguishable at the genome‐wide level. Polygenic risk scores (PRS) may therefore improve diagnostic classification.METHODSWe assessed diagnostic classification using AD‐PRS excluding APOE (AD‐PRSnoAPOE), APOE risk score (APOE‐RS), and plasma phosphorylated tau 181 (p‐tau181), in 83 participants with LBD, 27 with positron emission tomography amyloid beta (Aβ)positive mild cognitive impairment or AD (MCI+/AD), and 57 controls.RESULTSTogether AD‐PRSnoAPOE and APOE‐RS performed similarly to p‐tau181 in discriminating MCI+/AD from controls (area under the curve 76% vs. 79%) and LBD (71% vs. 72%). In LBD, Aβ positivity was significantly associated with APOE‐RS, but not with AD‐PRSnoAPOE, or p‐tau181. Combining AD‐PRSnoAPOE, APOE‐RS, and p‐tau181 improved the discrimination of MCI+/AD from controls (81%) and LBD (75%), and the detection of Aβ in LBD (82%).DISCUSSIONAβ deposition in LBD was associated with APOE, while MCI+/AD was also associated with AD‐PRS beyond APOE. AD‐PRS explains phenotypic variance not captured by APOE or p‐tau181.Highlights
We investigated Alzheimer's disease (AD) polygenic risk score (PRS), apolipoprotein E (APOE), and plasma phosphorylated tau 181 (p‐tau181) to classify AD and Lewy body dementia (LBD).
AD‐PRS with APOE achieved similar classification accuracy to p‐tau181.
AD‐PRS without APOE significantly contributed to discriminating AD from LBD.
Amyloid beta positivity in LBD was associated with APOE but not AD‐PRS without APOE or p‐tau181.
Combining AD‐PRS, APOE, and p‐tau181 improved diagnostic classification accuracy.
Enantioselective Trifluoromethylazidation of Styrenyl Olefins Catalyzed by an Engineered Nonheme Iron Enzyme
Authors: He Hua , Yan Jia‐Xin , Zhu Jian‐Xiang , Liu Si‐Jia , Liu Xiao‐Qi , Chen Peng , Wang Xin , Jia Zhi‐Jun 
Published: January 2025

AbstractOrganofluorines, particularly those containing trifluoromethyl (CF3) groups, play a critical role in medicinal chemistry. While trifluoromethylation of alkenes provides a powerful synthetic route to construct CF3‐containing compounds with broad structural and functional diversity, achieving enantioselective control in these reactions remains a formidable challenge. In this study, we engineered a nonheme iron enzyme, quercetin 2,3‐dioxygenase from Bacillus subtilis (BsQueD), for the enantioselective trifluoromethylazidation of alkenes. Through directed evolution, the final variant BsQueD‐CF3 exhibited excellent enantioselectivity, with an enantiomeric ratio (e.r.) of up to 98 : 2. Preliminary mechanistic studies suggest the involvement of radical intermediates. This work expands biocatalytic organofluorine chemistry by reprogramming metalloenzymes for innovative trifluoromethylation reactions.
Preparation of a Highly Effective PMIA/BaTiO3 Nanofiber Membrane for Particulate Matter Removal Under High Temperature
Authors: Chen Mingxing , Sun Jiawang , Hu Qian , Wang Xinya , Zhang Wei , Li Yonggui 
Published: January 2025

ABSTRACTParticulate matter (PM) pollution is widely recognized as a major threat to public health, making the development of thermally stable filters for particulate removal in high‐temperature environments critically important. However, the common polymer‐based nanofiber air filters are inadequate stability when exposed to high temperature. In this study, the poly (m‐phenylene isophthalamide) (PMIA) and nano barium titanate (BaTiO3) which showed remarkable thermal endurance was utilized to prepare the PMIA/BaTiO3 composite nanofiber air filter. The structure and performance of nanofibers were regulated by changing the addition of BaTiO3. The PMIA/BaTiO3 nanofiber air filter with nano‐protrusion structure exhibits high PM2.5 filtration efficiency (98.7%) and low pressure drop (60.4 Pa). Even after being exposed to treatment at 250°C, the PMIA/BaTiO3 composite nanofiber air filter can still maintain stable filtration performance. The results showed that PMIA/BaTiO3 nanofiber materials hold promise for eliminating particulate matter in high‐temperature environments.
Properties of Recycled ABS and HIPS Polymers From WEEE and Their Blends With Virgin ABS Prepared by 3D Printing and Compression Molding
Authors: de Sousa Filho Válmer Azevedo , de Azevedo Ana Caroline Santana , de Oliveira Rafaela , Bonfim Ryan Lucas Pereira , da Silva Amaral Anny Karine , da Cunha Rafael Braga , Agrawal Pankaj , Cunha Carlos Thiago Candido , de Figueiredo Brito Gustavo , de Mélo Tomás Jeferson Alves 
Published: January 2025

ABSTRACTThe rapid increase in waste electrical and electronic equipment (WEEE) poses a significant environmental challenge. To address this issue, initiatives promoting circular economy principles have emerged, such as utilizing recycled acrylonitrile butadiene styrene (ABSr) and high‐impact polystyrene (HIPSr) from WEEE. This study evaluated the properties of virgin ABS (ABSv)/ABSr and ABSv/HIPSr blends, with ABSr and HIPSr obtained from TV housing WEEE. The blend samples were prepared using filament extrusion, followed by either compression molding or 3D printing. ABSv exhibited a stronger shear‐thinning behavior than ABSr and HIPSr at low shear rates, indicating a higher content of butadiene rubber. The viscosities of the blends increased with higher ABSv content at low shear rates and approximated those of ABSv, ABSr, and HIPSr at high shear rates. Overall, compression‐molded blends demonstrated superior viscosities at low shear rates and higher impact strength compared to their 3D–printed counterparts.
Is cranial anatomy indicative of fossoriality? A case study of the mammaliaform Hadrocodium wui
Authors: Tumelty Molly , Lautenschlager Stephan 
Published: January 2025

AbstractDetermining the ecology of fossil species presents considerable challenges due to the often fragmentary preservation of specimens. The mammaliaform Hadrocodium wui from the Jurassic of China is known only from the cranium and mandible but may have had a fossorial lifestyle. It shares morphological similarities with talpid moles and soricid shrews and is closely related to other fossorial mammaliaforms. However, the lack of postcranial elements has so far precluded a definitive assessment regarding its fossorial behavior. Using a combination of geometric morphometric analysis of the lower mandible and finite element analyses of the cranium, comparisons between H. wui and extant groups are made. H. wui resembles talpid moles more closely than shrews in mandible shape. However, there are more similarities between H. wui and semi‐fossorial/semi‐aquatic moles than fully fossorial moles. The skull of H. wui is particularly weak in every tested biomechanical scenario when compared to the related mammaliaform Morganucodon oehleri and talpid moles. This weakness is potentially a result of the reduction in thickness of the zygomatic arch. In contrast, the shrew crania show similar stress magnitudes and distribution. These results imply that H. wui was not fully fossorial and fed on a diet of softer invertebrates. Skull morphology may therefore not be sufficient to infer fossoriality in forelimb‐digging species.
Construction of Axially Chiral Tetra‐ortho‐Substituted Biaryls by Palladium‐Catalyzed Asymmetric Suzuki‐Miyaura Coupling
Authors: Qu Hong‐Yu , Zheng Wen‐Hua 
Published: January 2025

AbstractAxial chiral biaryl skeletons are widely found in biologically active molecules, catalysts and chiral functional materials. However, highly catalytic stereoselective synthesis of tetra‐ortho‐substituted biaryls remains a challenging task. In this paper, we describe an efficient approach for construction of axially tetra‐ortho‐substituted biaryls via Suzuki‐Miyaura coupling in the presence of a chiral monophosphate ligand developed by ourselves. This method provides a variety of products in good enantioselectivities and good yields (up to 90.7 : 9.3 er and 82 % yield) under mild conditions. Further control experiments indicate that the chiral side chain is crucial to the enantioselectivity.
Amelioration of biased neuronal differentiation in humanized mouse model of valproic acid‐induced autism by precisely targeted transcranial magnetic stimulation
Authors: Hou Yilin , Zhao Youyi , Yang Dingding , Feng Tingwei , Li Yuqian , Li Xiang , Liu Zhou'an , Yan Xiao , Zhang Hui , Wu Shengxi , Liu Xufeng , Wang Yazhou 
Published: January 2025

AbstractAutism spectrum disorder (ASD) is a group of developmental diseases, which still lacks effective treatments. Pregnant exposure of Valproic acid (VPA) is an important environmental risk factor for ASD, but it's long‐term effects on the development of human neural cells, particularly in vivo, and the corresponding treatment have yet been fully investigated. In the present study, we first made a humanized ASD mouse model by transplanting VPA‐pretreated human neural progenitor cells (hNPCs) into the cortex of immune‐deficient mice. In comparison with wild type and control chimeric mice, ASD chimeric mice (VPAhNPC mice) exhibit core syndromes of ASD, namely dramatic reduction of sociability, social interaction and social communication, and remarkable increase of stereotype repetitive behaviors and anxiety‐like behaviors. At cellular level, VPA‐pretreatment biased the differentiation of human excitatory neurons and their axonal projections in host brain. Chemogenetic suppression of human neuronal activity restored most behavior abnormalities of VPAhNPC mice. Further, specific modulation of human neurons by a newly developed transcranial magnetic stimulation (TMS) device which could precisely target hPNCs effectively recued the core syndromes of ASD‐like behaviors, restored the excitatory‐inhibitory neuronal differentiation and axonal projection, and reversed the expression of over half of the VPA‐affected genes. These data demonstrated that VPAhNPC mice could be used as a humanized model of ASD and that precisely targeted TMS could ameliorate the VPA‐biased human neuronal differentiation in vivo.
Perceived Benefits and Barriers of Gardening Among Urban Older Adults: A Chinese Perspective
Authors: Lin Peilin , Morris Paul Graham , Williams Joanne 
Published: January 2025

ABSTRACTExisting research exploring perspectives on gardening has primarily focused on Western populations. The present study, employing qualitative semi‐structured interviews with 21 urban‐dwelling Chinese older adults, investigated their perceived benefits and barriers to gardening. Reflexive thematic analysis of interview transcripts revealed that urban Chinese older adults perceive gardening as beneficial to their living environment, physical, psychological and social wellbeing, and as a source of home‐grown products. Perceived barriers include the lack of suitable gardening sites, insufficient time and support, difficulties in obtaining materials, the inseparability of gardening and living areas and low self‐efficacy in gardening. Cultural and socially specific influences are reflected in gardening practices, such as plant symbolism, household structures, and residential conditions, highlighting the importance of locally tailored strategies for future gardening programmes.
Comprehensive Analysis of Conventional and Bioactive Compositions of the Exocarp of Five Mainly‐Planted Ginkgo biloba Varieties
Authors: Zhang Fang , Wang Huimin , Wu Rong , Shi Manman , Dong Qihui , Su Erzheng 
Published: January 2025

ABSTRACTGinkgo biloba exocarp, a by‐product of seed production, is produced in an amount of over 75,000 tons annually in China. However, due to the lack of suitable processing methods, it is predominantly discarded as agricultural waste, resulting in a substantial waste of resources. To seek new development directions, this study first comprehensively evaluated the conventional and bioactive compositions of the exocarps of the five mainly planted G. biloba varieties. The results indicated that G. biloba exocarps contained some preponderant bioactive compositions, including ginkgolic acids (54.10–85.42 mg/g), proanthocyanidins (26.62–52.96 mg/g), and polysaccharides (29.72–51.72 mg/g). G. biloba exocarps were also abundant in terpene trilactones (332.63–780.31 µg/g), flavonoids (70.88–109.26 µg/g), and 6‐hydroxykynurenic acid (43.19–302.99 µg/g). The conventional components of G. biloba exocarps were mainly ash, crude fiber, and fat. The contents of starch and protein were lower; thus, G. biloba exocarp exhibits a lower nutritional value. G. biloba exocarps have the potential to be the best resource for the preparation of ginkgolic acids (GAs), proanthocyanidins, and polysaccharides. This study provides a theoretical basis for the development and utilization of G. biloba exocarps.