bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using artificial intelligence based language interpretation in non-urgent paediatric emergency consultations: a clinical performance test and legal evaluation
Authors: Brandenberger Julia , Stedman Ian , Stancati Noah , Sappleton Karen , Kanathasan Sarathy , Fayyaz Jabeen , Singh Devin 
Published: January 2025

Abstract

Objective
To evaluate the accuracy of Google Translate (GT) in translating low-acuity paediatric emergency consultations involving respiratory symptoms and fever, and to examine legal and policy implications of using AI-based language interpretation in healthcare.

Methods
Based on the methodology used for conducting language performance testing routinely at the Interpreter Services Department of the Hospital for Sick Children, clinical performance testing was completed using a paediatric emergency scenario (child with respiratory illness and fever) on five languages: Spanish, French, Urdu, Arabic, and Mandarin. The study focused on GT's translation accuracy and a legal and policy evaluation regarding AI-based interpretation in healthcare was conducted by legal scholars.

Results
GT demonstrated strong translation performance, with accuracy rates from 83.5% in Urdu to 95.4% in French. Challenges included dialect sensitivity and pronoun misinterpretations. Legal evaluation indicated inconsistent access to language interpretation services across healthcare jurisdictions and potential risks involving data privacy, consent, and malpractice when using AI-based translation tools.

Conclusions
Google Translate can effectively support communication in specific non-critical paediatric emergency scenarios. However, its use necessitates careful monitoring, understanding of its limitations, and attention to dialect and literal translation risks along with equity considerations. Establishing legal and policy frameworks for language interpretation in healthcare is crucial, alongside addressing funding and data security concerns, to optimize the use of AI-based translation tools in healthcare contexts.
Spatial transcriptome reveals histology-correlated immune signature learnt by deep learning attention mechanism on H&E-stained images for ovarian cancer prognosis
Authors: Ng Chun Wai , Wong Kwong-Kwok , Lawson Barrett C. , Ferri-Borgogno Sammy , Mok Samuel C. 
Published: January 2025

Abstract

Background
The ability to predict the prognosis of patients with ovarian cancer can greatly improve disease management. However, the knowledge on the mechanism of the prediction is limited. We sought to deconvolute the attention feature learnt by a deep learning convolutional neural networks trained with whole-slide images (WSIs) of hematoxylin-and-eosin (H&E)–stained tumor samples using spatial transcriptomic data.

Methods
In this study, 773 WSIs of H&E-stained tumor sections from 335 patients with treatment naïve high-grade serous ovarian cancer who were included in The Cancer Genome Atlas (TCGA) Pan-Cancer study were used to train, and validate, and to test a ResNet101 CNN model modified with attention mechanism. WSIs from patients in an independent cohort were used to further evaluate the model.

Results
The prognostic value of the predicted H&E-based survival scores from the trained model on patient survival was evaluated. The attention signals learnt by the model were then examined their correlation with immune signatures using spatial transcriptome. After validating the model with the testing datasets, pathway enrichment analysis showed that the H&E—based survival score significantly correlated with certain immune signatures and this was validated spatially using spatial transcriptome data generated from ovarian cancer FFPE samples by correlating the selected signature and attention signal.

Conclusions
In conclusion, attention mechanism might be useful to identify regions for their specific immune activities. This could guide future pathological study for the useful immunological features that are important in modulating the prognosis of ovarian cancer patients.
Associations between CAG repeat size, brain and spinal cord volume loss, and motor symptoms in spinocerebellar ataxia type 3: a cohort study
Authors: Ye Zhi-Xian , Chen Xuan-Yu , Li Meng-Cheng , Chen Xin-Yuan , Qiu Yu-Sen , Yuan Ru-Ying , Chen Zhi-Li , Lin Min-Ting , Hu Jian-Ping , Fu Ying , Chen Wan-Jin , Wang Ning , Gan Shi-Rui ,   on behalf of the OSCCAR Investigators
Published: January 2025

Abstract

Background
Spinocerebellar ataxia type 3 (SCA3) is a hereditary disease caused by abnormally expanded CAG repeats in the ATXN3 gene. The study aimed to identify potential biomarkers for assessing therapeutic efficacy by investigating the associations between expanded CAG repeat size, brain and spinal cord volume loss, and motor functions in patients with SCA3.

Methods
In this prospective, cross-observational study, we analyzed 3D T1-weighted MRIs from 92 patients with SCA3 and 42 healthy controls using voxel-based morphometry and region of interest approaches. Associations between expanded CAG repeat size, brain and spinal cord volume loss, and International Cooperative Ataxia Rating Scale (ICARS) scores were investigated using partial correlation and mediation analyses. Sample sizes of potential biomarkers were calculated.

Results
Compared with healthy controls, SCA3 patients had lower cerebellar volume and cervical spinal cord area. SCA3 patients evolved along a stage-independent decline that began in the cerebellum, progressed to spinal cord, brainstem, thalami, and basal ganglia, and extensive subcortex. Expanded CAG repeat size was associated with right cerebellar lobule IV volume (r = − 0.423, P < 0.001) and cervical spinal cord area (r = − 0.405, P < 0.001), and higher ICARS (r = 0.416, P < 0.001). Mediation analysis revealed an indirect effect of expanded CAG repeat size on ICARS through spinal cord. Sample sizes estimation revealed that a minimum sample size was achieved with spinal cord measures.

Conclusions
Our results indicate the potential of cervical spinal cord area as a biomarker for disease progression and a minimum sample size estimation in future clinical studies of SCA3.
Targeting UGT2B15 and NR1H4 interaction: a novel therapeutic strategy for polycystic ovary syndrome using naftopidil enantiomers
Authors: Zheng Xiufen , Chen Zikai , Liang Miao , Zhou Liting , Wang Miaoru , Zhang Silin , Zhang Shuyun , Ma Lei , Yi Wei , Liu Xiawen 
Published: January 2025

Abstract

Background
Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder among women of reproductive age. It is characterized by hyperandrogenism, ovulatory dysfunction, and the presence of polycystic ovarian morphology (PCOM) on ultrasound, often accompanied by metabolic disturbances such as insulin resistance and obesity. Current treatments, including oral contraceptives and anti-androgen medications, often yield limited efficacy and undesirable side effects. This study investigates the role of UGT2B15, an essential enzyme for androgen metabolism, in PCOS pathogenesis and its potential as a therapeutic target.

Methods
We used RNA sequencing to examine the effects of UGT2B15 knockdown in KGN cells. To modulate UGT2B15 expression, we employed siRNA and (R)/(S)-NAF (naftopidil), a chemical inducer of UGT2B15 identified in our previous studies on a prostate hyperplasia model. The effects of siRNA and (R)/(S)-NAF on dihydrotestosterone (DHT) levels, cell apoptosis, and the expression of apoptosis-related proteins in KGN cells were evaluated. In a PCOS mouse model, we assessed the effects of (R)-NAF and (S)-NAF on serum androgen levels, menstrual cycles, ovarian morphology, and UGT2Bs expression. Additionally, luciferase reporter and ChIP assays were utilized to study UGT2B15 regulation by NR1H4.

Results
Elevated androgens were found to suppress UGT2B15 expression in ovarian granulosa cells, leading to DHT accumulation and apoptosis. (R)-NAF and (S)-NAF treatments reversed these effects, alleviating PCOS symptoms in mice such as hyperandrogenism, irregular menstrual cycles, and the presence of ovarian cysts. NR1H4 negatively regulated the transcription of UGT2B15 in KGN cells. (R)-NAF and (S)-NAF disrupted NR1H4 binding to the UGT2B15 promoter without affecting its protein levels, indicating direct interference with its regulation.

Conclusions
UGT2B15 represents a promising target for novel PCOS therapies by modulating androgen metabolism and protecting ovarian granulosa cells from apoptosis. (R)-NAF and (S)-NAF regulate UGT2B15 by disrupting NR1H4's binding to its promoter, implying potential therapeutic compounds for PCOS treatment.
Mitochondrial base editing: from principle, optimization to application
Authors: Tang Jinling , Du Kunzhao 
Published: January 2025

Abstract
In recent years, mitochondrial DNA (mtDNA) base editing systems have emerged as bioengineering tools. DddA-derived cytosine base editors (DdCBEs) have been developed to specifically induce C-to-T conversion in mtDNA by the fusion of sequence-programmable transcription activator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs), and split deaminase derived from interbacterial toxins. Similar to DdCBEs, mtDNA adenine base editors have been developed with the ability to introduce targeted A-to-G conversions into human mtDNA. In this review, we summarize the principles of mtDNA base-editing systems and elaborate on the evolution of different platforms of mtDNA base editors, including their deaminase replacement, engineering of DddAtox variants, structure optimization and editing outcomes. Finally, we highlight their applications in animal models and human embroys and discuss the future developmental direction and challenges of mtDNA base editors.
Neutrophil extracellular traps in tumor metabolism and microenvironment
Authors: Liu Zhanrui , Dou Yuanyao , Lu Conghua , Han Rui , He Yong 
Published: January 2025

Abstract
Neutrophil extracellular traps (NETs) are intricate, web-like formations composed of DNA, histones, and antimicrobial proteins, released by neutrophils. These structures participate in a wide array of physiological and pathological activities, including immune rheumatic diseases and damage to target organs. Recently, the connection between NETs and cancer has garnered significant attention. Within the tumor microenvironment and metabolism, NETs exhibit multifaceted roles, such as promoting the proliferation and migration of tumor cells, influencing redox balance, triggering angiogenesis, and driving metabolic reprogramming. This review offers a comprehensive analysis of the link between NETs and tumor metabolism, emphasizing areas that remain underexplored. These include the interaction of NETs with tumor mitochondria, their effect on redox states within tumors, their involvement in metabolic reprogramming, and their contribution to angiogenesis in tumors. Such insights lay a theoretical foundation for a deeper understanding of the role of NETs in cancer development. Moreover, the review also delves into potential therapeutic strategies that target NETs and suggests future research directions, offering new perspectives on the treatment of cancer and other related diseases.
Age-related mortality risk in cycling trauma: analysis of the National Trauma Databank 2017–2023
Authors: Rowh Marta A. W. , Giller Taylor A. , Bliton John N. , Smith Randi N. , Moran Tim P. 
Published: January 2025

Abstract

Background
Cycling promotes health but carries significant injury risks, especially for older adults. In the U.S., cycling fatalities have increased since 1990, with adults over 50 now at the highest risk. As the population ages, the burden of cycling-related trauma is expected to grow, yet age-specific factors associated with mortality risk remain unclear. This study identifies age-specific mortality risk thresholds to inform targeted public health strategies.

Methods
We conducted a cross-sectional analysis of the National Trauma Data Bank (NTDB) data (2017–2023) on non-motorized cycling injuries. A total of 185,960 records were analyzed using logistic regression with splines to evaluate the relationship between age and mortality risk. The dataset was split into training (80%) and testing (20%) sets. Age thresholds where mortality risk changed were identified, and models were adjusted for injury severity, comorbidities, and helmet use.

Results
The median patient age was 43 years (IQR 20–58). Four key age thresholds (12, 17, 31, and 69) were identified, with the largest mortality increase after age 69. Our model achieved an AUC of 0.93, surpassing traditional age cutoff models, with 84.6% sensitivity and 88.0% specificity.

Conclusions
Age is a significant predictor of mortality in cycling trauma, with marked increases in risk during adolescence and for adults over 69. These findings underscore the need for age-targeted interventions, such as improved cycling infrastructure for teens and enhanced safety measures for older adults. Public health initiatives should prioritize these vulnerable age groups to reduce cycling-related mortality.
The greenhouse gas observation mission with Global Observing SATellite for Greenhouse gases and Water cycle (GOSAT-GW): objectives, conceptual framework and scientific contributions
Authors: Tanimoto Hiroshi , Matsunaga Tsuneo , Someya Yu , Fujinawa Tamaki , Ohyama Hirofumi , Morino Isamu , Yashiro Hisashi , Sugita Takafumi , Inomata Satoshi , Müller Astrid , Saeki Tazu , Yoshida Yukio , Niwa Yosuke , Saito Makoto , Noda Hibiki , Yamashita Yousuke , Ikeda Kohei , Saigusa Nobuko , Machida Toshinobu , Frey Matthias Max , Lim Hyunkwang , Srivastava Priyanka , Jin Yoshitaka , Shimizu Atsushi , Nishizawa Tomoaki , Kanaya Yugo , Sekiya Takashi , Patra Prabir , Takigawa Masayuki , Bisht Jagat , Kasai Yasko , Sato Tomohiro O. 
Published: January 2025

Abstract
The Japanese Global Observing SATellite for Greenhouse gases and Water cycle (GOSAT-GW) will be an Earth-observing satellite to conduct global observations of atmospheric carbon dioxide (CO2), methane (CH4), and nitrogen dioxide (NO2) simultaneously from a single platform. GOSAT-GW is the third satellite in the series of the currently operating Greenhouse gases Observing SATellite (GOSAT) and GOSAT-2. It will carry two sensors, the Total Anthropogenic and Natural emissions mapping SpectrOmeter-3 (TANSO-3) and the Advanced Microwave Scanning Radiometer 3 (AMSR3), with the latter dedicated to the observation of physical parameters related to the water cycle. TANSO-3 is a high-resolution grating spectrometer designed to measure reflected sunlight in the visible to short-wave infrared spectral ranges. It aims to retrieve the column-averaged dry-air mole fractions of CO2 and CH4 (denoted as XCO2 and XCH4, respectively), as well as the vertical column density of tropospheric NO2. The TANSO-3 sensor onboard GOSAT-GW will utilize the wavelength bands of 0.45, 0.76, and 1.61 µm for NO2, O2, and CO2 and CH4 retrievals, respectively. GOSAT-GW will fly in a sun-synchronous orbit with a local overpass time of approximately 13:30 and a 3-day ground-track repeat cycle. The TANSO-3 sensor has two observation modes in the push-broom operation: Wide Mode, which provides globally covered maps with a 10-km spatial resolution within 3 days, and Focus Mode, which provides snapshot maps over targeted areas with a high spatial resolution of 1–3 km. The objectives of the GOSAT-GW mission include (1) monitoring atmospheric global-mean concentrations of greenhouse gasses (GHGs), (2) verifying national anthropogenic GHG emissions inventories, and (3) detecting GHG emissions from large sources, such as megacities and power plants. A comprehensive validation exercise will be conducted to ensure that the sensor products’ quality meets the required precision to achieve the above objectives. With a projected operational lifetime of seven years, GOSAT-GW will provide vital space-based constraints on both anthropogenic and natural GHG emissions. These measurements will contribute significantly to climate change mitigation efforts, particularly by supporting the Global Stocktake (GST) mechanism, a key element of the Paris Agreement.
Feasibility and Safety of Field-Based Physical Fitness Tests: A Systematic Review
Authors: Cruz-León Carolina , Expósito-Carrillo Pablo , Sánchez-Parente Sandra , Jiménez-Iglesias José , Borges-Cosic Milkana , Cuenca-Garcia Magdalena , Castro-Piñero José 
Published: January 2025

Abstract

Background
While there is evidence on the validity and reliability of field-based physical fitness tests in children, adolescents and adults, there is limited evidence to provide feasibility and safety data on the application and performance of the existing field-based physical fitness tests.

Objectives
(i) To examine the feasibility and safety of existing field-based physical fitness tests used in people of all ages and (ii) to establish a comprehensive view of criterion-related validity, reliability, feasibility and safety based on scientific evidence for the existing field-based physical fitness tests in adults.

Methods
The search was conducted through the electronic databases MEDLINE (via PubMed) and Web of Science (all databases) for published studies from inception to 31 January 2023. This systematic review was developed according to Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Studies were classified as very low quality, low quality or high quality, based on the criteria of appropriate number of participants, appropriate description of the study population, and appropriate number of items reported to assess feasibility/safety. Three evidence levels were constructed (strong, moderate and limited or inconclusive evidence) according to the number of studies and the consistency of the findings.

Results
We identified a total of 19 (14 of high quality) and 13 (11 high quality) original studies examining the feasibility and safety of field-based physical fitness tests, respectively. Strong evidence indicated that (a) the 2-km walk and 20-m shuttle run tests were feasible in adults and, children and adolescents, respectively; (b) the handgrip strength test was feasible in children and adolescents; and (c) the standing long jump test was feasible in children and adolescents. Only the 2-km walk test has shown strong evidence on safety in adults. Finally, combining the levels of evidence of criterion-related validity, reliability, feasibility and safety in adults, all the field-based physical fitness tests show limited evidence.

Conclusion
There is a need for more studies and consensus to establish homogeneous methodological criteria to assess the feasibility and safety of field-based fitness tests. The combined evidence on criterion-related validity, reliability, feasibility and safety of field-based tests was found to be limited in adults.

PROSPERO reference number CRD42022298276.
Smokeless tobacco: knowledge, attitudes and usage in Pakistan
Authors: Irshad Hammad Atif , Raja Sajjan , Jehanzeb Hamzah , Shaikh Wamiq Ali , Saleem Umair , Malik Shahzil Abdur Rehman , Shahzad Akmal , Shaikh Mamoon , Iqbal Nousheen , Khan Javaid Ahmed 
Published: January 2025

Abstract

Background
Smokeless tobacco (SLT) encompasses products that are not burnt but instead consumed orally or nasally. One-third of tobacco is consumed in the smokeless form in South Asia. Despite its widespread usage, there has been limited empirical research on the prevalence and factors influencing SLT consumption in Pakistan. This study aimed to provide an assessment of SLT knowledge, attitudes, and usage in Pakistan.

Methods
Using an online questionnaire, a cross-sectional survey was conducted across Pakistan. Convenience sampling was used to disseminate, and expert approval was sought followed by a pilot study to validate the questionnaire. A comprehensive questionnaire was developed using elements from the Global Adult Tobacco Survey and other tools which had previously been utilized within Pakistan. Participants’ responses were described using descriptive statistics, and SPSS version 26 was used to perform linear and logistic regression. A p-value of less than 0.05 was considered statistically significant.

Results
Data was collected from 1,195 participants among which 85.86% of participants had heard of SLTs prior to the survey. Sex, province, household income and previous doctor visits were significant determinants to product knowledge through which 72.55% agreed that these products can lead to serious illnesses but 30% noted a lack of knowledge on legality. General attitudes agreed that it makes one feel unwell (44.1%), gives a sense of guilt (43.85%) and more research is needed on it (54.39%). Negative health effects were the biggest deterrent from pursuing these products for 60.33% of participants. Among significant factors influencing the use of SLTs, the greatest odds of use were found with use as an alternative to cigarettes (OR 4.45) and secondly, due to its availability in a liked flavor (OR 2.27). About 31% reported to have used the product once, with 69.82% of current users expressing a desire to quit.

Conclusions
Although adequate knowledge and aligning attitudes exist for SLTs, light is shed in the face of a sustaining public health problem. We offer important insights that can guide the creation of focused interventions meant to lower SLT use, and the health hazards associated with it in the Pakistani community by highlighting important myths, incentives, and deterrents.
What could make seaports more committed to air quality? A qualitative content analysis of key barriers, drivers, and enablers
Authors: Gulyás Flóra Zsuzsanna , Kinra Aseem 
Published: January 2025

Abstract

Background
There is a growing need for seaports to find ways to reduce the air pollution they generate. A wide range of potential best practices exist, but what lies behind the decision to implement them? Prior studies have focused on either barriers, drivers, or enablers in this context. Thus, the purpose of this paper is to review existing research on various measures to reduce air pollution in seaports with the aspects of both barriers and enablers that influence the implementation of these actions.

Methods
To answer the research question, the authors conducted a systematic literature review using the databases of Web of Science (WoS) and Scopus. The initial search identified 247 studies in WoS and 589 studies in Scopus. After applying exclusion and inclusion criteria, a qualitative content analysis (QCA) was performed to narrow down the resulting articles and extract relevant information. The identified factors were then categorized, followed by a factor frequency analysis (FFA).

Results
Based on the final selection of 48 research articles, the review identified and categorized a total of 38 barriers and 28 enabling factors. The barriers were grouped into economic, technical, managerial, informational, and regulatory categories. The enabling factors were grouped into economic-financial, regulations and policies, informational, professional, and training categories. Aspects of finance and regulation are highlighted by the FFA.

Findings
Financial aspects play a crucial role on both the barriers and enablers side, while aspects such as information or professional training seem to be neglected. Barriers are diverse, while enablers focus mostly on regulations. With our findings, we contribute to previous work that will serve as a basis for further research on the interdependencies of influencing factors, as well as experts and stakeholders in developing suitable emission reduction (ER) strategies for citizens living and working in and around seaports. The ranking of importance as well as the degree of influence of the various factors depend on further aspects to be considered.