bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drought risk assessment by applying drought hazard and vulnerability indices for irrigated wheat (Case study: Khorasan provinces, Iran)
Authors: Latifi Hoda , Khorramdel Soroor , Koocheki Alireza , Bannayan Mohammad , Farzaneh Mahmood-Reza 
Published: November 2023

Abstract
This study investigated drought risk, drought vulnerability, and drought hazard in Northeast Iran (North Khorasan, Razavi Khorasan, and South Khorasan provinces) during 2009-2018. Exposure, sensitivity and adaptive capacity together determine the agricultural drought vulnerability. Three parameters of soil water holding capacity, level of mechanization and the amount of water consumption of agricultural wells were considered effective adaptive capacity factors to reduce the negative effects of drought on irrigated wheat production by having an auxiliary role. Results showed that among 51 counties in Khorasan provinces, 12 counties are exposed to the low drought Vulnerability, 11 counties to the moderate drought Vulnerability and 28 counties to the high drought Vulnerability. Counties with less drought vulnerability have a high score in terms of the adaptive capacity index. This high score is due to the high water holding capacity of the soil in these areas. Regions in the high and very high drought hazard classes are mostly in the center and south of the studied region and the amount of precipitation is low. In general, irrigated wheat is produced with medium and high risk in half of the counties of North Khorasan, Razavi Khorasan and South Khorasan provinces. Water scarcity, lack of reliable surface sources, improper distribution of rainfall, high temperature and poor vegetation cover in these areas have caused many problems in agriculture. Risk management needs to be revised in these regions due to the undeniable effects of climate change and the strategic nature of this product.
Impact of Bisphenol A on the levels of vascular calcification biomarkers in Type 2 Diabetes Mellitus with vascular complications: A Case-control Study
Authors: Nehru Mohanraj , S Jancy M , Durairaj Prabhu , S Kumar J , Janardhanan Rajiv , Prabhu Venkataraman 
Published: November 2023

Abstract
Bisphenol A (BPA) is a chemical that disrupts the endocrine system and is found in various plastic products that are routinely used. Recent research suggests that BPA develops insulin resistance, which results in type 2 diabetes mellitus (T2DM), which also causes vascular complications. Vascular calcification (VC) is the primary concern in T2DM and diabetic vascular complications. Fetuin-A (FTA) and Osteoprotegerin (OPG) are the VC biomarkers in T2DM. The association of BPA with T2DM and its associated vascular complications are limited in human studies. Our study aims to associate systemic BPA levels with FTA and OPG in T2DM and diabetic vascular complications. Diabetic vascular complications such as cardiovascular disease (CVD) and diabetic nephropathy (DN) were confirmed by carotid intima-media thickness (CIMT) and urine microalbuminuria (UMA), respectively. Serum and urinary FTA, OPG and BPA levels were measured by enzyme-linked immunosorbent assay (ELISA) kit. The biochemical parameters were performed using standard protocols. We found increased levels of serum (p < 0.001) and urinary BPA (p < 0.001) in T2DM, along with lower levels of serum and urinary FTA (p < 0.001) and enhanced levels of OPG (p < 0.001) in the study groups. Serum and urinary BPA levels were highly associated with serum FTA (p < 0.001), urinary FTA (p < 0.01), serum OPG (p < 0.001) and urinary OPG (p < 0.001) in our study groups. Our study demonstrates an association between increased serum and urinary BPA levels, poor diabetic and lipid profile, and insulin resistance. It is especially significant with lower FTA and enhanced OPG in patients with T2DM and its associated vascular complications.
BCMA-targeted CAR-T Therapy Associated Adverse Events in Multiple Myeloma: A Systematic Review and Meta-analysis
Authors: Jian Hou , Fu Xuehang , Yang Jingwen , Yu Dandan , Jin Shikai , Zhang Liwen , Chen Zhenwei , Du Jun 
Published: November 2023

Abstract
B cell maturation antigen (BCMA)-targeted chimeric antigen receptor modified (CAR)-T therapy is an emerging treatment option for multiple myeloma (MM) but many severe adverse events (AEs) remain in clinical practice, raising safety concerns regarding this promising therapy. In this systematic review, we searched 4 databases and selected 45 reports from 32 different studies involving a total of 927 patients with MM. Data were extracted and analyzed to assess the efficacy and safety of the therapy. Complete responses (CR) and stringent complete responses (sCR) were achieved in 48% (95% CI, 39%-59%) patients, with partial responses and very good partial responses (VGPR) were achieved in 36% (95% CI, 30%-43%) patients. Of the patients who had MM evaluable for minimal residual disease (MRD), 71% (95% CI: 56%-89%) achieved negative status for bone marrow MRD. Regarding safety, the AE with the highest incidence was cytokine release syndrome (CRS), which occurred in 76% (95% CI, 65%-86%) patients, while severe CRS was observed in 10% (95% CI, 5%-14%) patients. The AE with the second highest incidence was neurotoxicity reported in 9% (95% CI, 5%-14%) patients, with the proportion of severe neurotoxicity being 2% (95% CI, 1%-4%). Notably, hematological toxicities, often manifesting as cytopenia, were a prominent severe AE. Furthermore, 68 (7.34%) of the 927 enrolled patients had died. This study shws that, despite the promising results of BCMA-targeted CAR-T therapy in MM patients, significant and sometimes severe toxicities were observed frequently during treatment. There is an urgent need to place more emphasis on these AEs and solutions. The study was registered in PROSPERO, number CRD42022295858.
Postoperative pain of minimally invasive root canal treatment:A Randomized Clinical Trial
Authors: Kim Do-hyun , Choi Yoon-woo , Kang Sumi , Shin Su-Jung , Jung Il-Young 
Published: November 2023

Abstract
Background
This randomized clinical trial compared postoperative pain between a minimally invasive (MP) and conventional root canal treatment protocol (CP).
Methods
A total of 170 mature permanent teeth (either with vital or necrotic pulp), were randomly assigned into two groups. In the CP group, ProTaper Gold (Dentsply Sirona, Ballaigues, Switzerland) and a continuous wave of condensation technique were used, whereas, in the MP group, TruNatomy (Dentsply Sirona), ultrasonic-assisted irrigation (UI), calcium hydroxide (CH), and a sealer-based obturation technique were used. Patients recorded preoperative and postoperative pain using a 0–10 numerical rating scale (NRS) at 4 h, 1, 2, 3, 4, 5, 6, and 7 days after instrumentation and 1 day after canal obturation, respectively.
Results
There were no significant differences in pain intensity at any time points assessed between the two groups (p > 0.05). The occurrence of moderate/intense pain after instrumentation was significantly associated with preoperative periapical index (PAI) (p = 0.017) and NRS scores (p< 0.001). Preoperative pulp status (p = 0.009) and NRS score (p = 0.006) were identified as significant factors in the occurrence of moderate/intense pain after obturation.
Conclusions
Instrumentation unequivocally reduced pain severity for both groups. The post-endodontic pain associated with the use of MP, combined with UI, CH, and calcium-silicate cement, did not differ from that of CP. Preoperative pain score, PAI, and preoperative pulp status were determined to be prognostic factors for postoperative pain.
Trial registration:
The study was registered at the Clinical Research Information Service (CRIS, no. KCT0005351: 25/08/2020).
Molecular Set Representation Learning
Authors: Probst Daniel , Boulougouri Maria , Vandergheynst Pierre 
Published: November 2023

Abstract

Computational representation of molecules can take many forms, including graphs, string-encodings of graphs, binary vectors, or learned embeddings in the form of real-valued vectors. These representations are then used in downstream classification and regression tasks using a wide range of machine-learning models. However, existing models come with limitations, such as the requirement for clearly defined chemical bonds, which often do not represent the true underlying nature of a molecule. Here, we propose a framework for molecular machine learning tasks based on set representation learning. We show that learning on sets of atomic invariants alone reaches the performance of state-of-the-art graph-based models on the most-used chemical benchmark data sets and that introducing a set representation layer into graph neural networks can surpass the performance of established methods in the domains of chemistry, biology, and material science. We introduce specialised set representation-based neural network architectures for reaction yield and protein-ligand binding affinity prediction. Overall, we show that the technique we denote molecular set representation learning is both an alternative and an extension to graph neural network architectures for machine learning tasks on molecules, molecule complexes, and chemical reactions.
Protective effect of conditioned medium from brain pericytes overexpressing telomerase reverse transcriptase on hypoxic-ischemic neurons
Authors: Liu Shixi , Huang Lingyi , Zheng Zizhuo , Zhang Mingfu , Li Hui , Zhao Fengyan , Wang Shaopu , Su Xiaojuan , Li Shiping , Ying Junjie , Liu Qian , Qu Yi 
Published: November 2023

Abstract
Aims: Cerebral microvascular pericytes can secrete both neurotrophic factors and neurotoxic molecules, which together construct the microenvironment for nerve growth and repair. The aim of this study is to detect the effect of telomerase reverse transcriptase (TERT) overexpression on the change of secretory spectrum of brain pericytes, and to examine the protective effect and mechanism of conditioned medium from brain pericytes overexpressing TERTon hypoxic-ischemic neurons.
Methods:TERT overexpressing pericytes were constructedand the conditioned medium was collected. Cortical neurons weresubjected to oxygen-glucose deprivation (OGD) and cultured in neurobasal/B27 or conditioned medium from pericytes, andtheir survival and apoptosis were detected.Furthermore, conditioned medium was analyzed using Tandem Mass Tagstechnology to examine the differentially expressed proteins. Then these proteins were analyzedand the key proteins related to neuronal protectionwere selected and verified.
Results: Conditioned medium from pericytes increased survival and decreased apoptosis of OGD neurons, and TERT over-expression enhanced this effect. The quantitative proteomics of conditioned mediumselected 12 differentially expressedproteins which were related to neuronal protection, among them FGF2 and apoE4 were determined as the possible effectors to regulate neuronal survival inconditioned medium from brain pericytes overexpressing TERT through verification experiments. Mechanistically, TERT regulated the expression of FGF2 and ApoE4 through activating Akt signaling.
Conclusion: TERT overexpression enhanced the neuroprotective effect of conditioned mediumthrough improving the secretion profile of pericytes. Conditioned medium from brain pericytes overexpressing TERT has the potential to be developed as an effective neuroprotective agent.
Adapting Strengthening Families Program into Brazilian online version: Formative evaluation of family-based prevention
Authors: Pietrobon Tania Vilela , Garcia-Cerde Rodrigo , Valente Juliana Yurgel , Sanchez Zila van der Meer 
Published: November 2023

Abstract
Online family drug prevention programs have been shown to affect the behavior of children and adolescents. This study presents the formative evaluation results of the online implementation of Familias Fortes, a Brazilian-adapted version of the Strengthening Families Program (SFP 10–14). A total of 31 families participated in the evaluation, and data were collected from 74 observations, 64 fidelity forms, 20 focus groups, and 16 semi-structured interviews in groups located in five cities with a total of 113 participants. The evaluation focused on three outcomes: fidelity, appropriateness, and feasibility. Low fidelity was observed, with videos and audio content not consistently sent as planned, although most core elements were maintained. The program's appropriateness showed good accessibility and utilization of video content by participants, but confidentiality was lacking in adolescent meetings. Feasibility analysis revealed challenges related to poor internet access, malfunctioning of facilitator cameras, issues with time management and internal organization of institutions, and insufficient facilitator training. Specific problematic activities were identified, and recommendations for improvement were made. These included enhancing internet connectivity, developing a new manual specific to the online format, improving time management for facilitators, providing more interactive facilitator training, and considering a hybrid format. By implementing these adaptations and conducting a pilot evaluation, an online version of Familias Fortes could be disseminated in Brazil as a pilot model until its effectiveness is evaluated.
Influence of Biomechanical Stimulus on 3D Bioprinted Respiratory Tissue Scaffolds
Authors: Zimmerling Amanda , Boire Jim , Zhou Yan , Chen Xiongbiao 
Published: November 2023

Abstract
In respiratory tissue engineering, the development of respiratory tissue models or constructs is been challenging due to the complex structure of native respiratory tissue and the unique biomechanical conditions induced by breathing. While studies have shown that the inclusion of biomechanical stimulus that mimics physiological conditions greatly benefits the development of engineered tissues, to our knowledge no studies investigating the influence of biomechanical stimulus on the development of respiratory tissue models engineered by three-dimensional (3D) bioprinting have been reported. This paper presents a study on the inclusion of biomechanical stimulus during the culture of 3D bioprinted constructs for the development of respiratory tissue models with further investigation into the influence of biomechanical stimulus on the tissue constructs in terms of mechanical and biological properties. Constructs were bioprinted using an alginate/collagen hydrogel containing human primary pulmonary fibroblasts with further seeding of human primary bronchial epithelial cells. Biomechanical stimulus was then applied via a novel ventilated incubator developed for our study. Constructs were subject to four different pressure change and airflow conditions: standard incubation, shallow breathing, normal breathing and heavy breathing, over a two-week time period. At time points of 1, 3, 5, 7, 10, and 14 days, constructs were examined and characterized in terms of tensile mechanical properties, cell proliferation and cell morphology. The results illustrated that conditions mimicking normal and heavy breathing led to greater and more continuous cell proliferation, and greater production of vimentin, a structural protein produced by fibroblasts. Inclusion of biomechanical stimulus during culture of 3D bioprinted respiratory constructs promotes cellular growth and proliferation, indicating a more physiologically relevant respiratory tissue model.
Instant Gaze (IG): An Interaction Driven Adaptive Gaze Control Interface
Authors: Qian Kun , Arichi Tomoki , Edwards A. David , Hajnal Joseph V 
Published: November 2023

Abstract
Gaze estimation is long been recognised as having potential as the basis for human-computer interaction (HCI) systems, but usability and robustness of performance remain challenging . This work focuses on systems in which there is a live video stream showing enough of the subjects face to track eye movements and some means to infer gaze location from detected eye features. Currently, systems generally require some form of calibration or set-up procedure at the start of each user session. Here we explore some simple strategies for enabling gaze based HCI to operate immediately and robustly without any explicit set-up tasks. We explore different choices of coordinate origin for combining extracted features from multiple subjects and the replacement of subject specific calibration by system initiation based on prior models. Results show that referencing all extracted features to local coordinate origins determined by subject start position enables robust immediate operation. Combining this approach with an adaptive gaze estimation model using an interactive user interface enables continuous operation with the 75th percentile gaze errors of 0.7 degrees, and maximum gaze errors of 1.7 degrees during prospective testing. There constitute state-of-the-art results and have the potential to enable a new generation of reliable gaze based HCI systems.
Simultaneous fault detection and control for a class of nonlinear uncertain systems with limited communication
Authors: Cao Hongyan , Zhang Juanmei , Wang Guoyong , Ji Zhihang 
Published: November 2023

Abstract
This paper investigates the simultaneous fault detection and control(SFDC) problem for a class of uncertain nonlinear networked control systems with limited communication.
In order to improve the utilization of network resources, a novel dynamic event-triggered mechanism is employed affected by the Bernoulli package dropouts phenomenon, which is usually appearing in practical systems. Then, a fault detection filter and dynamic output feedback controller module is applied to achieve the required fault detection and control objectives simultaneously.
The linear matrix inequality-based approach is provided to obtain the designed filter and controller parameters so that the closed-loop system is robustly stochastically stable and meets the required performance.
Finally, two practical examples are adopted to show the effectiveness of the developed scheme.
Antiaging Synergistic Effect in Noninvasive Transdermal Delivery of Peptide Loaded Liposomes by Low Energy/Frequency Radiofrequency
Authors: Xiang Nanxi , Huang Zeting , Zhang Chunqiao , Huang Jiahong , Wang Zhenyuan , Zhang Jichuan , Wu Chengyu , Zhang Jiaheng , Peng Weihua 
Published: November 2023

Abstract
Low energy/frequency radiofrequency (LRF) combined with the transdermal delivery of liposome (L) encapsulated antiaging peptides technology is a remarkable, newly developed physical noninvasive transdermal penetration technique; it is considered a highly efficient, comprehensive and safe technology. In this study, our objective was to evaluate the physical and chemical mechanisms underlying the efficacy of this innovative technique involving a combination of LRF and L, termed LLRF, that exerts a synergistic anti-aging effect on human skin, via an animal experiment. Physical and chemical analyses indicated that a relatively stable liposome with a uniform nano-size, which was formed, possessed good transdermal permeability that was 2.74 folds higher than that of the free peptide (F). LLRF exhibited a higher transdermal permeation performance that was of 3.65 folds higher than that of the free one, which was substantiated via confocal laser scanning fluorescence microscopy. The mouse UVB photoaging model trial confirmed that the LLRF technology exerted a significant synergistic effect compared to liposome technology, or free peptide, by downregulating inflammatory factors (IL-6, TNF-α), inhibiting the mRNA and protein expression of matrix metalloproteinases (MMP1, MMP3), promoting the mRNA and protein expression of related collagens (Procollagen, Col1α1 and Col3α1), and repairing the stratum corneum barrier function, as evidenced by trans-epidermal water loss (TEWL), skin cuticle hydration (SCH), and decreased expression of β-gal, an aging marker. These findings indicated that photoaging skin can be effectively and comprehensively rejuvenated, and that even photodamage can be reversed, thereby restoring the original physiological characteristics of healthy skin. Clinical tests have confirmed that although liposome technology is an effective antiaging method which helps exert tightening and anti-wrinkle effects on human skin, LLRF is an even more effective anti-aging technique. This study reveals a highly effective technique involving a combination physical and chemical therapy that may be utilized for antiaging purposes as well as repairing lightly damaged skin, and can be made readily available in the future.
Examining factors associated with vaccine hesitancy against Covid-19 among adults in Europe: a secondary analysis of cross-sectional survey data
Authors: Nagase Megumi 
Published: November 2023

Abstract
Objective
This study aimed to explore the factors associated with Covid-19 vaccine hesitancy in Europe among adults by using the Socio-Ecological Model.
Results
This cross-sectional study used secondary data collected from respondents residing in 27 EU countries at the time of May 2021. The outcome was vaccine hesitancy against Covid-19, and the total sample size of 23,606 was analysed by binary logistic regression, as well as McKelvey and Zavonoia’s R2. After adding each level of variables, the model found the significant and increased association with vaccine hesitancy in younger age groups (21-39 years and 40-60 years vs 65 years+), who left full-time education at a young age (16-19 years), those with manual jobs, those with children at home, individuals residing in small towns, and norms related to the vaccine. Together, the levels explained 49.4% of the variance associated with vaccine hesitancy, and the addition to each variable layer increased the variance. This highlights the need to consider broad factors at multiple levels to enhance vaccine acceptance and uptake.
Genetic and microbial determinants of azoxymethane-induced colorectal tumor susceptibility in Collaborative Cross mice and their implication in human cancer
Authors: Li Dan , Zhong Chenhan , Yang Mengyuan , He Li , Chang Hang , Zhu Ning , Celniker Susan E , Threadgill David W , Snijders Antoine M , Mao Jian-Hua , Yuan Ying 
Published: November 2023

Abstract

Background
The insights into interactions between host genetics and gut microbiome (GM) in colorectal tumor susceptibility (CTS) remains lacking. We used Collaborative Cross mouse population model to identify genetic and microbial determinants of Azoxymethane-induced CTS.
Results
We identified 4417 CTS-associated SNPs containing 936 known human genes, 334 of which were found to be transcriptionally altered in human CRCs. Transcriptional expression of 334 CTS genes clustered human CRCs into two subgroups with prognostic impact in independent cohorts. We discovered a set of genera in early-life associated with CTS and defined a 16-genus signature that predicted CTS, the majority of which were correlated with human CRCs. We identified 547 SNPs significantly associated with the abundances of at least 5 of 16 genera. Mediation analysis revealed GM as mediators exerting genetic effects on CTS, whereby Ruminococcus, Akkermansia, Allobaculum, Bacteroides, Sutterella, and Bifidobacterium mediate the effect of UNC3869242 within Duox2 on CTS. Intestine cell-specific depletion of Duox2 altered GM composition and contribution of Duox2 depletion to CTS was significantly influenced by GM.
Conclusions
This study identifies genetic and microbial determinants of azoxymethane-induced CTS, shows critical importance of interaction between genetics and microbiome in CTS, and provides potential novel targets for personalized CRC prevention and treatment.