bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Decline in Intergenerational Mobility after 1980
Authors: Davis Jonathan M.V. , Mazumder Bhashkar 
Published: February 2024

Abstract
Relative intergenerational mobility declined for cohorts born around 1960 compared to those born around 1950. The former entered the labor market after the rise in inequality around 1980 while the latter entered the labor market earlier. We show that the rank-rank slope rose from 0.25 to 0.36 and the intergenerational elasticity increased from 0.28 to 0.45. These increases are more pronounced for men than for women. Increases in returns to schooling and in the gradient in the likelihood of marriage by parent income are contributors to increased intergenerational persistence.
Assessment of allelic Mi-1.2 dosage effects on levels of resistance to virulent and avirulent Meloidogyne spp. populations in some tomato rootstocks
Authors: Gabriel Márcia , Santos Marcilene F.A. , Mattos Vanessa S. , Almeida Sheila F. , Boiteux Leonardo S. , Carneiro Regina M.D.G. 
Published: February 2024

Summary
The -1.2 gene confers broad-spectrum resistance to populations of at least 13 Meloidogyne species, being present in a wide range of commercial tomato (Solanum lycopersicum) cultivars. A more refined assessment of the allelic dosage effects of the -1.2 gene to virulent and avirulent Meloidogyne populations may provide relevant information for the management of the durability of the resistance under field conditions. Herein, the allelic dosage effects (homozygous vs heterozygous at the -1.2 locus) on the phenotypic expression of resistance were evaluated against one -1.2-virulent and six avirulent Meloidogyne spp. populations. Seven tomato rootstocks with distinct allelic conditions at the -1.2 locus and one susceptible control (‘Santa Clara’) were used in the present study. Significant dosage effects of -1.2 alleles in reducing the reproduction factors (RF) were observed, but restricted to the avirulent Meloidogyne populations. Homozygous resistant (-1.2/-1.2) cultivars, except TD1, prevented reproduction of almost avirulent Meloidogyne spp. populations in contrast with the heterozygous allele combination (-1.2/-1.2), which allowed a certain level of nematode reproduction. The efficiency and durability of the -1.2 gene depend on its proper management under commercial field conditions. Heterozygous (-1.2/-1.2) plants will increase the population of Meloidogyne spp., with no drastic reduction as occurring in the case of homozygous resistant, and they allow a small reproduction of more adapted sub-populations of the nematode, which might increase over time. Therefore, our results strongly indicated that the employment (whenever possible) of hybrids carrying the double dosage of the -1.2 gene would reduce the potential of high nematode populations in plants with this allelic configuration.
Der fünfmal getötete Pfarrer
Authors: Schneidergruber Dietmar 
Published: February 2024

Abstract
This essay establishes a connection between the late medieval fairy tale Der fünfmal getötete Pfarrer and the penitential of John of Erfurt. A critical examination of the state of research takes place and an alternative is offered. The tale can also be a critical examination of church rules that are ridiculously pushed to their limits.
Assimilation of Water Vapor Retrievals from ZDR Columns Using the 3DVar Method for Improving the Short-Term Prediction of Convective Storms
Authors: Chen Haiqin , Gao Jidong , Sun Tao , Chen Yaodeng , Wang Yunheng , Carlin Jacob T. 
Published: February 2024

Abstract
The differential reflectivity (ZDR) column is a notable polarimetric signature related to updrafts in deep moist convection. In this study, pseudo–water vapor (qυ) observations are retrieved from observed ZDR columns under the assumption that humidity is saturated within the convection where ZDR columns are detected, and are then assimilated within the 3DVar framework. The impacts of assimilating pseudo-qυ observations from ZDR columns on short-term severe weather prediction are first evaluated for a squall-line case. Radar data analysis indicates that the ZDR columns are mainly located on the inflow side of the high-reflectivity region. Assimilation of the pseudo-qυ observations leads to an enhancement of qυ within the convection, while concurrently reducing humidity in no-rain areas. Sensitivity experiments indicate that a tuned smaller observation error and a shorter horizontal decorrelation scale are optimal for a better assimilation of pseudo-qυ from ZDR columns, resulting in more stable rain rates during short-term forecasts. Additionally, a 15-min cycling assimilation frequency yields the best performance, providing the most accurate reflectivity forecast in terms of both location and intensity. Analysis of thermodynamic fields reveal that assimilating ZDR columns provides more favorable initial conditions for sustaining convection, including sustainable moisture condition, a strong cold pool, and divergent winds near the surface, consequently enhancing reflectivity and precipitation. With the optimal configuration determined from the sensitivity tests, a quantitative evaluation further demonstrates that assimilating the pseudo-qυ observations from ZDR columns using the 3DVar method can improve the 0–3-h reflectivity and accumulated precipitation predictions of convective storms.
Influence of Perceptual Load on Attentional Orienting in Post-Stroke Fatigue: A Study of Auditory Evoked Potentials
Authors: De Doncker William , Kuppuswamy Annapoorna 
Published: February 2024

Objective Increasing perceptual load alters behavioral outcomes in post-stroke fatigue (PSF). While the effect of perceptual load on top-down attentional processing is known, here we investigate if increasing perceptual load modulates bottom-up attentional processing in a fatigue dependent manner. Methods In this cross-sectional observational study, in 29 first-time stroke survivors with no clinical depression, an auditory oddball task consisting of target, standard, and novel tones was performed in conditions of low and high perceptual load. Electroencephalography was used to measure auditory evoked potentials. Perceived effort was rated using the visual analog scale at regular intervals during the experiment. Fatigue was measured using the fatigue severity scale. The effect of fatigue and perceptual load on behavior (response time, accuracy, and effort rating) and auditory evoked potentials (amplitude and latency) was examined using mixed model ananlysis of variances (ANOVA). Results Response time was prolonged with greater perceptual load and fatigue. There was no effect of load or fatigue on accuracy. Greater effort was reported with higher perceptual load both in high and low fatigue. p300a amplitude of auditory evoked potentials (AEP) for novel stimuli was attenuated in high fatigue with increasing load when compared to low fatigue. Latency of p300a was longer in low fatigue with increasing load when compared to high fatigue. There were no effects on p300b components, with smaller N100 in high load conditions. Interpretation High fatigue specific modulation of p300a component of AEP with increasing load is indicative of distractor driven alteration in orienting response, suggestive of compromise in bottom-up selective attention in PSF.
Mn–Fe-rich genthelvite from pegmatites associated with the Madeira Sn–Nb–Ta deposit, Pitinga, Brazil: new constraints on the magmatic-hydrothermal transition in the albite-enriched granite system
Authors: Hadlich Ingrid W. , Bastos Neto Artur C. , Pereira Vitor P. , Botelho Nilson F. , Ronchi Luiz H. , Dill Harold G. 
Published: February 2024

AbstractGenthelvite from pegmatite veins hosted by the albite-enriched granite (ca.1.8 Ga) corresponding to the Sn–Nb–Ta (F, REE, Li, Zr, U, Th) Madeira deposit, Amazonas, Brazil was studied. Genthelvite, the exclusive Be-bearing mineral within the deposit, occurs as massive crystals of up to 4.7 cm in size. Compositions are homogeneous within individual crystals, although there is moderate variation in the overall composition reflecting relatively limited substitutions within the helvine–genthelvite–danalite solid-solution series, with relatively high Zn contents (36.96 to 49.45 wt.% ZnO), lower Mn contents (0.61 to 3.03 wt.% MnO), and variable Fe contents (2.10 to 10.94 wt.% FeO), completing an existing compositional gap in this system. Genthelvite formed in an alkaline and subaluminous environment, under stable conditions within the late-evolved fluids, at relatively high temperature (>400°C), in a reducing environment. The extremely high concentration of fluorine in the magma and the crystallisation of magmatic galena resulted in an effective reduction of H2S fugacity. This resulted in the stabilisation of genthelvite during the transition from the late magmatic to early hydrothermal stages of the albite-enriched granite evolution. The variability in Fe content within genthelvite is associated primarily with localised variations in the mineral assemblage (e.g. the presence of riebeckite and polylithionite). Genthelvite was altered by low-temperature aqueous fluids rich in F which resulted in the incorporation of Fe, Mn, Mg, Pb, Ba, Na, K, U and REE into the Zn2+ structural site and the allocation of excess Si, Al, Ti and P in the IVSi and IVBe structural sites. The substantial content of U and REE substituting for Zn, together with Si substituting for Be, is charge balanced by the presence of vacancies at the A site.
Investigating plant responses to microgravity and adaptations in gravisensitive environments
Authors: Farooq Muhammad , Ali Sajid , Khan Murtaza , Jang Yoon-Hee , Kim Eun-Gyeong , Zhao Dan-Dan , Kim Kyung-Min 
Published: February 2024

AbstractPlants are crucial because they give us food and oxygen. With the idea of living on other planets and taking long trips in space, we need to understand and explore the way how plants can survive in these strange places. However, while the gravity response on earth’s surface has been extensively studied in plants, in space, where the gravity is very weak, things get confusing. One of the intriguing and essential subjects for space life is understanding how plants can sustain themselves in microgravity conditions. To investigate this, various clinostat devices and the CRISPR/Cas9 technique are crucial tools for exploring the functioning of PIN-formed protein and related signal transduction pathways. In this review, we aim to provide researchers with a brief overview of the mechanisms of CRISPR/Cas9, which can be immensely helpful when using this method alongside clinostat machines. Our primary goal in this review is to address the missing gaps in existing literatures, focusing on how plants perceive gravity and experimental approaches applicable for studying their responses to microgravity, both on earth and in space.
Metagenomic analysis of gut microbiome illuminates the mechanisms and evolution of lignocellulose degradation in mangrove herbivorous crabs
Authors: Hui Tom Kwok Lun , Lo Irene Ching Nam , Wong Karen Ka Wing , Tsang Chandler Tsz To , Tsang Ling Ming 
Published: February 2024

Abstract
Background
Sesarmid crabs dominate mangrove habitats as the major primary consumers, which facilitates the trophic link and nutrient recycling in the ecosystem. Therefore, the adaptations and mechanisms of sesarmid crabs to herbivory are not only crucial to terrestrialization and its evolutionary success, but also to the healthy functioning of mangrove ecosystems. Although endogenous cellulase expressions were reported in crabs, it remains unknown if endogenous enzymes alone can complete the whole lignocellulolytic pathway, or if they also depend on the contribution from the intestinal microbiome. We attempt to investigate the role of gut symbiotic microbes of mangrove-feeding sesarmid crabs in plant digestion using a comparative metagenomic approach.

Results
Metagenomics analyses on 43 crab gut samples from 23 species of mangrove crabs with different dietary preferences revealed a wide coverage of 127 CAZy families and nine KOs targeting lignocellulose and their derivatives in all species analyzed, including predominantly carnivorous species, suggesting the crab gut microbiomes have lignocellulolytic capacity regardless of dietary preference. Microbial cellulase, hemicellulase and pectinase genes in herbivorous and detritivorous crabs were differentially more abundant when compared to omnivorous and carnivorous crabs, indicating the importance of gut symbionts in lignocellulose degradation and the enrichment of lignocellulolytic microbes in response to diet with higher lignocellulose content. Herbivorous and detritivorous crabs showed highly similar CAZyme composition despite dissimilarities in taxonomic profiles observed in both groups, suggesting a stronger selection force on gut microbiota by functional capacity than by taxonomy. The gut microbiota in herbivorous sesarmid crabs were also enriched with nitrogen reduction and fixation genes, implying possible roles of gut microbiota in supplementing nitrogen that is deficient in plant diet.

Conclusions
Endosymbiotic microbes play an important role in lignocellulose degradation in most crab species. Their abundance is strongly correlated with dietary preference, and they are highly enriched in herbivorous sesarmids, thus enhancing their capacity in digesting mangrove leaves. Dietary preference is a stronger driver in determining the microbial CAZyme composition and taxonomic profile in the crab microbiome, resulting in functional redundancy of endosymbiotic microbes. Our results showed that crabs implement a mixed mode of digestion utilizing both endogenous and microbial enzymes in lignocellulose degradation, as observed in most of the more advanced herbivorous invertebrates.
Bacterial community structure and co-occurrence networks in the rhizosphere and root endosphere of the grafted apple
Authors: Cao Hui , Xu Longxiao , Song Jianfei , Xun Mi , Zhang Weiwei , Yang Hongqiang 
Published: February 2024

Abstract
Background
Compared with aerial plant tissues (such as leaf, stem, and flower), root-associated microbiomes play an indisputable role in promoting plant health and productivity. We thus explored the similarities and differences between rhizosphere and root endosphere bacterial community in the grafted apple system.

Results
Using pot experiments, three microhabitats (bulk soil, rhizosphere and root endosphere) samples were obtained from two-year-old apple trees grafted on the four different rootstocks. We then investigated the bacterial community composition, diversity, and co-occurrence network in three microhabitats using the Illumina sequencing methods. Only 63 amplicon sequence variants (ASVs) out of a total of 24,485 were shared in the rhizosphere and root endosphere of apple grafted on the four different rootstocks (M9T337, Malus hupehensis Rehd., Malus robusta Rehd., and Malus baccata Borkh.). The core microbiome contained 8 phyla and 25 families. From the bulk soil to the rhizosphere to the root endosphere, the members of the phylum and class levels demonstrated a significant enrichment and depletion pattern. Co-occurrence network analysis showed the network complexity of the rhizosphere was higher than the root endosphere. Most of the keystone nodes in both networks were classified as Proteobacteria, Actinobacteriota and Bacteroidetes and were low abundance species.

Conclusion
The hierarchical filtration pattern existed not only in the assembly of root endosphere bacteria, but also in the core microbiome. Moreover, most of the core ASVs were high-abundance species, while the keystone ASVs of the network were low-abundance species.
Antivirulence effects of cell-free culture supernatant of endophytic bacteria against grapevine crown gall agent, Agrobacterium tumefaciens, and induction of defense responses in plantlets via intact bacterial cells
Authors: Etminani Faegheh , Harighi Behrouz , Bahramnejad Bahman , Mozafari Ali Akbar 
Published: February 2024

Abstract
Background
Crown gall disease caused by Agrobacterium tumefaciens is a very destructive affliction that affects grapevines. Endophytic bacteria have been discovered to control plant diseases via the use of several mechanisms. This research examined the potential for controlling crown gall by three endophytic bacteria that were previously isolated from healthy cultivated and wild grapevines including Pseudomonas kilonensis Ba35, Pseudomonas chlororaphis Ba47, and Serratia liquefaciens Ou55.

Result
At various degrees, three endophytic bacteria suppressed the populations of A. tumefaciens Gh1 and greatly decreased the symptoms of crown gall. Furthermore, biofilm production and motility behaviors of A. tumefaciens Gh1were greatly inhibited by the Cell-free Culture Supernatant (CFCS) of endophytic bacteria. According to our findings, CFCS may reduce the adhesion of A. tumefaciens Gh1 cells to grapevine cv. Rashe root tissues as well as their chemotaxis motility toward the extract of the roots. When compared to the untreated control, statistical analysis showed that CFCS significantly reduced the swimming, twitching, and swarming motility of A. tumefaciens Gh1. The findings demonstrated that the endophytic bacteria effectively stimulated the production of plant defensive enzymes including superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL), and total soluble phenols at different time intervals in grapevine inoculated with A. tumefaciens Gh1. The Ba47 strain markedly increased the expression levels of defense genes associated with plant resistance. The up-regulation of PR1, PR2, VvACO1, and GAD1 genes in grapevine leaves indicates the activation of SA and JA pathways, which play a role in enhancing resistance to pathogen invasion. The results showed that treating grapevine with Ba47 increased antioxidant defense activities and defense-related gene expression, which reduced oxidative damage caused by A. tumefaciens and decreased the incidence of crown gall disease.

Conclusion
This is the first study on how A. tumefaciens, the grapevine crown gall agent, is affected by CFCS generated by endophytic bacteria in terms of growth and virulence features. To create safer plant disease management techniques, knowledge of the biocontrol processes mediated by CFCS during microbial interactions is crucial.
Comparison of statistical methods used to meta-analyse results from interrupted time series studies: an empirical study
Authors: Korevaar Elizabeth , Turner Simon L. , Forbes Andrew B. , Karahalios Amalia , Taljaard Monica , McKenzie Joanne E. 
Published: February 2024

Abstract
Background
The Interrupted Time Series (ITS) is a robust design for evaluating public health and policy interventions or exposures when randomisation may be infeasible. Several statistical methods are available for the analysis and meta-analysis of ITS studies. We sought to empirically compare available methods when applied to real-world ITS data.

Methods
We sourced ITS data from published meta-analyses to create an online data repository. Each dataset was re-analysed using two ITS estimation methods. The level- and slope-change effect estimates (and standard errors) were calculated and combined using fixed-effect and four random-effects meta-analysis methods. We examined differences in meta-analytic level- and slope-change estimates, their 95% confidence intervals, p-values, and estimates of heterogeneity across the statistical methods.

Results
Of 40 eligible meta-analyses, data from 17 meta-analyses including 282 ITS studies were obtained (predominantly investigating the effects of public health interruptions (88%)) and analysed. We found that on average, the meta-analytic effect estimates, their standard errors and between-study variances were not sensitive to meta-analysis method choice, irrespective of the ITS analysis method. However, across ITS analysis methods, for any given meta-analysis, there could be small to moderate differences in meta-analytic effect estimates, and important differences in the meta-analytic standard errors. Furthermore, the confidence interval widths and p-values for the meta-analytic effect estimates varied depending on the choice of confidence interval method and ITS analysis method.

Conclusions
Our empirical study showed that meta-analysis effect estimates, their standard errors, confidence interval widths and p-values can be affected by statistical method choice. These differences may importantly impact interpretations and conclusions of a meta-analysis and suggest that the statistical methods are not interchangeable in practice.
Prognostic impacts of repeated sepsis in intensive care unit on autoimmune disease patients: a retrospective cohort study
Authors: Yang Jinming , Chen Jie , Zhang Min , Zhou Qingsa , Yan Bing 
Published: February 2024

Abstract
Background
Autoimmune diseases (ADs) may be complicated by sepsis when intensive care unit (ICU) admission. But repeated sepsis among AD patients has not been studied yet. The aim of this study is to investigate the impact of repeated in-ICU sepsis on the 1-year overall-cause mortality, septic shock and in-ICU death of AD patients.

Methods
Data of AD patients with sepsis retrieved from Medical Information Mart for Intensive Care IV (MIMIC-IV) database were divided into the single group and the repeated group according to the frequency of in-ICU sepsis. Propensity score matching was used to balance inter-group bias. Cox proportional hazard regression and sensitivity analysis were utilized to assess the variables on mortality.

Results
The incidence of repeated in-ICU sepsis in baseline was 19.8%. The repeated in-ICU sepsis was a risk factor for 1-year overall-cause mortality among AD patients (adjusted hazard ratio [HR] = 1.50, 95% CI: 1.16–1.93, P = 0.002), with robust adjusted HRs by the adjustment for confounders in the sensitivity analysis (all P < 0.01). Maximum Sequential Organ Failure Assessment (Max SOFA), Charlson comorbidity index (CCI) and Simplified Acute Physiology Score-II (SAPS-II) were risk factors for 1-year overall-cause mortality among AD with repeated sepsis (Max SOFA: HR = 1.09, P = 0.002; CCI: HR = 1.08, P = 0.039; SAPS-II: HR = 1.03, P < 0.001).

Conclusions
Compared to single hit, repeated in-ICU sepsis was independently related to a higher risk of 1-year overall-cause mortality among AD patients. Assessment tools (Higher SOFA, CCI and SAPS-II scores) were closely linked to poor prognosis of AD with repeated sepsis and helped to reflect ill physical conditions for the patients.