Penambahan Yolk Puyuh, Sari Pepaya (Carica papaya L.), Ringer’s serta Lama Penyimpanan pada Suhu 50C terhadap Kualitas Spermatozoa Ayam KUB
Authors: Wajo Muhammad Jen , Widayati Isti , Ratlalan Bryan 
Published: January 2024

Abstract
KUB chickens have a low volume of semen per ejaculate, but the concentration is high. IB volume and concentration requirements must be met. Papaya (Carica papaya L.), is rich in carbohydrates, protein, fat, fiber, antioxidants and vitamins. Quail yolk contains lipoprotein, fat, carbohydrates, minerals and vitamin A. The addition of papaya juice in ringer's lactate diluent + quail yolk as a diluent for KUB chicken semen is thought to improve the quality of KUB chicken spermatozoa during storage. Experimental research, using a Completely Randomized Factorial Design with two factors; the first factor is diluent with two levels, namely level I (ringer's lactate + quail yolk) and II (ringer's lactate + quail yolk + papaya juice); while the second factor is the length of time of storage in the refrigerator at 50C consisting of five levels namely 0, 2, 4, 6 and 8 hours. The research variables included volume, consistency, pH, color and odor, concentration of spermatozoa, motility of spermatozoa (%), viability of spermatozoa and abnormality of spermatozoa. The results showed that there was a significant interaction between adding papaya juice to ringer's diluent + quail yolk and storage time at 50C on the quality of semen (spermatozoa) of KUB chickens. The addition of papaya juice to ringer's diluent + quail yolk had a good effect on maintaining motility, viability and abnormal spermatozoa of KUB chickens. The length of storage time has a significant effect on the quality of semen (spermatozoa) of KUB chickens.
Keywords: Carica papaya; KUB chicken; Spermatozoa

Abstrak
Ayam KUB memiliki volume semen per ejakulat rendah, namun konsentrasinya tinggi. Syarat volume dan konsentrasi IB harus terpenuhi. Pepaya (Carica papaya L.), kaya kandungan karbohidrat, protein, lemak, serat, antioksidan, vitamin A, B1, B2, B3, B5, B6, asam folat, vitamin C, E dan K. Yolk puyuh mengandung lipoprotein, lemak, karbohidrat, mineral serta vitamin A. Penambahan sari buah pepaya dalam pengencer ringer’s laktat + yolk puyuh sebagai pengencer semen ayam KUB diduga dapat meningkatkan kualitas spermatozoa ayam KUB selama penyimpanan. Penelitian secara eksperimen, menggunakan Rancangan Acak Lengkap Faktorial (RAL-Faktorial) dengan 2 (dua) faktor; faktor pertama adalah bahan pengencer dengan 2 (dua) level yaitu level I (ringer’s laktat + yolk puyuh) dan II (ringer’s laktat + yolk puyuh + sari buah pepaya); sedangkan faktor kedua adalah lama waktu penyimpanan didalam refrigerator pada suhu 50C yang terdiri atas 5 (lima) level yaitu 0, 2, 4, 6 dan 8 jam. Variabel penelitian meliputi volume, konsistensi, pH, warna dan bau, konsentrasi spermatozoa, motilitas spermatozoa (%), viabilitas spermatozoa dan abnormalitas spermatozoa. Hasil penelitian menunjukkan bahwa ada interaksi yang signifikan antara penambahan sari buah pepaya pada pengencer ringer’s + yolk puyuh dan lama waktu penyimpanan pada suhu 50C terhadap kualitas semen (spermatozoa) ayam KUB. Penambahan sari buah pepaya pada pengencer ringer’s + yolk puyuh memberikan pengaruh yang baik dalam mempertahankan motilitas, viabilitas dan abnormalitas spermatozoa ayam KUB. Lama waktu penyimpanan memberikan pengaruh yang signifikan terhadap kualitas semen (spermatozoa) ayam KUB.
Kata kunci: Ayam KUB; Pepaya; Spermatozoa