bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Ip6k1 and Ip6k2 Kinases Are Critical for Normal Renal Tubular Function
Authors: Haykir Betül , Moser Seraina Olivia , Pastor-Arroyo Eva Maria , Schnitzbauer Udo , Radvanyi Zsuzsa , Prucker Isabel , Qiu Danye , Fiedler Dorothea , Saiardi Adolfo , Jessen Henning J. , Hernando Nati , Wagner Carsten A. 
Published: February 2024

Significance Statement
Kidneys are gatekeepers of systemic inorganic phosphate balance because they control urinary phosphate excretion. In yeast and plants, inositol hexakisphosphate kinases (IP6Ks) are central to regulate phosphate metabolism, whereas their role in mammalian phosphate homeostasis is mostly unknown. We demonstrate in a renal cell line and in mice that Ip6k1 and Ip6k2 are critical for normal expression and function of the major renal Na+/Pi transporters NaPi-IIa and NaPi-IIc. Moreover, Ip6k1/2

−/−
mice also show symptoms of more generalized kidney dysfunction. Thus, our results suggest that IP6Ks are essential for phosphate metabolism and proper kidney function in mammals.

Background
Inorganic phosphate is an essential mineral, and its plasma levels are tightly regulated. In mammals, kidneys are critical for maintaining phosphate homeostasis through mechanisms that ultimately regulate the expression of the Na+/Pi cotransporters NaPi-IIa and NaPi-IIc in proximal tubules. Inositol pyrophosphate 5-IP7, generated by IP6Ks, is a main regulator of phosphate metabolism in yeast and plants. IP6Ks are conserved in mammals, but their role in phosphate metabolism in vivo remains unexplored.

Methods
We used in vitro (opossum kidney cells) and in vivo (renal tubular-specific Ip6k1/2

−/−
mice) models to analyze the role of IP6K1/2 in phosphate homeostasis in mammals.

Results
In both systems, Ip6k1 and Ip6k2 are responsible for synthesis of 5-IP7. Depletion of Ip6k1/2 in vitro reduced phosphate transport and mRNA expression of Na+/Pi cotransporters, and it blunts phosphate transport adaptation to changes in ambient phosphate. Renal ablation of both kinases in mice also downregulates the expression of NaPi-IIa and NaPi-IIc and lowered the uptake of phosphate into proximal renal brush border membranes. In addition, the absence of Ip6k1 and Ip6k2 reduced the plasma concentration of fibroblast growth factor 23 and increased bone resorption, despite of which homozygous males develop hypophosphatemia. Ip6k1/2

−/−
mice also show increased diuresis, albuminuria, and hypercalciuria, although the morphology of glomeruli and proximal brush border membrane seemed unaffected.

Conclusions
Depletion of renal Ip6k1/2 in mice not only altered phosphate homeostasis but also dysregulated other kidney functions.
The Importance of Involving a Multidisciplinary Team in the Management of a Patient Diagnosed with TB, Associating Erythema Nodosum
Authors: Mihailov Oana Mariana , Mihailov Raul , Dinu Ciprian , Ciubara Alexandru Bogdan 
Published: February 2024

Tuberculosis (TB) is an important and topical problem at global level, so it remains a challenge for public health policies . According to the World Health Organization (WHO), in 2018, about 10 million new TB cases worldwide were estimated. Of these, 11% are children under the age of 15. In addition to these alarming data, TB is responsible for 130,000 deaths per year in children and it is estimated that there are approximately 1 million TB-cases in the global population of children, being one of the top 10 causes of death in children worldwide. According to the WHO, there are economic, social, cultural, ethnic/racial, psychological and behavioral factors that interfere with the occurrence of diseases, and these factors are attributed to the persistence of infectious diseases such as TB in the population, even today. Numerous specialized studies strengthen the hypothesis of associating social vulnerability with TB emergence and distribution in the community.
Therefore, the development of more accurate estimates of pediatric TB cases is an urgent need for public policy scheduling and resource allocation and, consequently, for reducing morbidity and mortality from TB among children.
The Influence of Physical Activity Regime on Body Composition in Teenagers With and Without Intellectual Disabilities
Authors: Puni Alexandru-Rareș , Cojocariu Adrian , Abalașei Beatrice-Aurelia , Popescu Lucian , Martinaș Florentina-Petruța , Bulduș Codruța-Florina , Tătăranu Elena , Popa Cristian , Ene-Voiculescu Carmen , Pârvu Carmen , Ungurean Bogdan-Constantin 
Published: February 2024

This study aims to assess several morphofunctional parameters in female teenagers with and without intellectual disability to characterise normality and its disturbances. More precisely, the purpose was to examine the factors contributing to the differences in body composition elements between groups, such as the physical activity level. To evaluate these parameters, we used bioelectrical impedance analysis technology (BIA), most commonly used in studies concerning body composition because it is non-invasive, quick, with high data fidelity; it can be easily moved to various locations and applied straightforwardly among populations with diverse types of intellectual disability. Our research sample comprised 212 subjects (boys and girls) aged 17.1±0.6, divided into six groups by gender and type of intellectual disability. The study found influences of physical activity level on body composition components. In addition, we report a highly significant relationship for p<0.05, body mass index and the independent variable (No. of min./week) in some groups.
A Survey of U.S. and Canadian Physicians’ Clinical Practices Related to Pharmacologic, Herbal, and Nutritional Substances Used to Increase Milk Production
Authors: Sadovnikova Anna , Sommers Jan , Oberhelman-Eaton Sara 
Published: February 2024

Objectives:Physicians may be asked to prescribe, recommend, or discuss foods, herbs, and medications for low milk supply. It is unclear how factors such as education, experience, and regulations influence clinical practice. The objective was to document physicians’ clinical practice surrounding pharmacologic, nutritional, and herbal products for the management of low milk supply.Methods:Physicians from two Facebook groups completed an anonymous, online survey about their clinical use of pharmaceutical, supplemental, and nutritional substances perceived to increase milk production. The association between practice patterns and lactation medicine expertise or country for different perceived galactagogues was determined.Results:A total of 307 physicians completed the primary portion of the survey. There was no difference in the proportion of physicians in Canada or the United States (U.S.) who were defined as lactation medicine experts. Canadian physicians were more likely to prescribe metformin, levothyroxine, and domperidone for low milk supply compared with U.S.-based physicians. Canadians and experts were more likely than U.S.-based and nonexpert physicians, respectively, to include herbs in their practice. Patient request, personal and clinical experience, and lack of education, research, or access were frequently selected as reasons for or against using a specific substance.Conclusions:The U.S. Food and Drug Administration ban on domperidone affects low milk supply management in North America. Though Canadian physicians prescribed domperidone without limitations, U.S. physicians were less likely to incorporate it into clinical practice. Despite a lack of evidence, physicians in North America frequently used herbs and foods to increase milk production.
PELATIHAN PENGISIAN E-SPT UNTUK PELAKU UMKM KOTA BANJARMASIN
Authors: Analisa  , Wahyuni  , Riri Velina Sari  
Published: February 2024

Kegiatan Pengabdian kepada Masyarakat (PKM) ini bertujuan untuk memberikan pelatihan bagi para pelaku UMKM dengan memberikan pemahaman tentang syarat, perhitungan dan tata cara pelaporan pajak penghasilan khususnya melalui e-SPT. Permasalahan mitra yang akan diselesaikan dalam kegiatan ini adalah sebagian UMKM di kota Banjarbaru dan sekitarnnya belum memahami tentang pengisian SPT dan penggunaan e-SPT untuk melaporkan kewajiban perpajakannya. Metode yang diterapkan untuk mengatasi masalah tersebut adalah dengan memberikan Pelatihan secara langsung untuk mengisi e-SPT. Kegiatan ini dibagi menjadi 3 tahap sesi perencanaan, pelaksanaan dan evaluasi. Dari hasil Kegiatan menunjukkan adanya peningkatan pemahaman dan pengetahuan setelah mengikuti kegiatan Pelatihan. Artinya, tujuan dari PKM ini telah tercapai. Rata-rata hasil angket kepuasan menunjukkan bahwa mitra (peserta) puas dengan materi, metode penyampaian, dan keseluruhan Pelatihan
Aplikasi Agensia Hayati Metarhizium anisopliae Terhadap Spodoptera frugiperda J. E. Smith
Authors: Ramadhani Roni , Soedijo Samharinto , Rosa Helda Orbani 
Published: February 2024

Fall Armyworm (Spodoptera frugiperda) is an invasive insect pest that has a high appetite, causing damage and reduced yields in corn plants. One way to control these larvae is to use the biological agent Metarhizium anisopliae. The aim of this research was to determine the influence of several spore densities of the entomopathogenic fungus M. anisopliae on S. frugiperda. The design used in this research was a Completely Randomized Design (CRD) which consisted of 4 treatment levels of spore suspension density 107,108, 109 and a water control treatment as a comparison with 6 replications. Application was carried out on 3-4 instar larvae with an observation period of 12 hours for 4 days. The results of this study showed that the application of the biological agent M. anisopliae with a spore density treatment of 109caused the highest larval mortality of 76.7%. Lethal time (LT_50) for larval mortality caused by M. anisopliae is 2.80 days. From probit analysis, the Lethal Concentration (LC_50) value was 2.44 x 109 spores/ml.
WITHDRAWN: Observation of 2D-magnesium-intercalated gallium nitride superlattices
Authors: Wang Jia , Cai Wentao , Lu Weifang , Honda Yoshio , Amano Hiroshi 
Published: February 2024

Abstract

We observe the intercalation of 2D-magnesium into singlecrystalline gallium nitride which occurs spontaneously between the two dissimilar materials under atmospheric pressure. This is the first-of-its-kind observation of intercalation of 2D-metal into bulk semiconductor in which every single monolayer of magnesium is inserted into a few monolayers of hexagonal gallium nitride without causing volume expansion. As a result, the unprecedented high elastic strain of over -10% amongst thin film materials is sustained in the intercalated gallium nitride layers which modified the electronic band structure and improved the hole mobility (e.g., over 200% increase at low temperature), indicating a good agreement with the long-sought phenomenon of the reversal of crystal-field splitting due to the ultra-small c/a ratio. The simultaneous improvement of hole mobility, as well as hole concentration due to the increased magnesium acceptors, is demonstrated after intercalating 2D-magnesium into p-type gallium nitride. These unique features may shed light on the doping and conductivity enhancement of semiconductors as well as the elastic strain engineering of nanomaterials. The observed layered structure also provides a novel probe for studying the band structure and transport properties of metal/semiconductor superlattices.
Hydrogenotrophic Methanogenesis at 7-12 mbar by Methanosarcina barkeri under Simulated Martian Atmospheric Conditions
Authors: Harris Rachel L , Schuerger Andrew C. 
Published: February 2024

Abstract

Mars, with its ancient history of long-lived habitable environments, continues to captivate researchers exploring the potential for extant life. This study investigates the viability of hydrogenotrophic methanogenesis, a potential biosignature, in Methanosarcina barkeri MS under simulated Martian surface conditions. We expose M. barkeri to sustained hypobaria (7–12 mbar), low temperature (0˚C), and a CO2-dominated gas mixture mimicking the Martian atmosphere. The results demonstrate statistically quantifiable CH4 production under all tested conditions, including at 7-12 mbar. Transcriptomics reveal that hypobaria alone did not significantly impact gene expression, highlighting the resilience of M. barkeri. However, atmospheric gas composition, specifically Mars gas with 2.9% pH2, led to significant down-regulation of methanogenesis genes, hindering growth over a 14-day experiment. Notably, CH4 production scaled with the partial pressure of H2, indicating its critical role in methanogenesis. We propose that Mars subsurface could harbor habitable refugia, sustaining microbial life at low metabolic steady states. The findings challenge assumptions about the habitability of present-day Mars and have implications for Planetary Protection and in situ resource generation for future human exploration. Overall, this work contributes to understanding the habitability of Mars and informs future astrobiological and exploration endeavors.
WITHDRAWN: Establishment of regeneration system from leaves wrapped by bud scales of Neolamarckia cadamba in the wild
Authors: Li Buye , Que Qingmin , Li Chunmei , Zhou Wei , Zhong Tianxiu , Chen Xiaoyang , Zhang Lifeng , Wang Rui , Xu Qixian , Chen Wenping , Zhong Ming , Huang Xiaoling , Ouyang Kunxi 
Published: February 2024

Abstract

The full text of this preprint has been withdrawn by the authors while they make corrections to the work. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Genome-wide association analysis identifies PLA2G4C as a susceptibility locus for Multiple System Atrophy
Authors: Tsuji Shoji , Nakahara Yasuo , Mitsui Jun , Date Hidetoshi , Porto Kristine Joyce , Hyashi Yasuhiro , Yamashita Atsushi , Kusakabe Yoshio , Matsukawa Takashi , Ishiura Hiroyuki , Yasuda Tsutomu , Iwata Atsushi , Goto Jun , Ichikawa Yaeko , Momose Yoshio , Takahashi Yuji , Toda Tatsushi , Ohta Rikifumi , Yoshimura Jun , Morishita Shinichi , Gustavsson Emil , Christy Darren , Maczis Melisa , Kim Han-Joon , Park Sung-Sup , Zhang Jin , Gu Weihong , Scholz Sonja , Chelban Viorica , Mok Kin , Houlden Henry , Yabe Ichiro , Sasaki Hidenao , Matsushima Masaaki , Takashima Hiroshi , Kikuchi Akio , Aoki Masashi , Hara Kenju , Kakita Akiyoshi , Yamada Mitsunori , Takahashi Hitoshi , Onodera Osamu , Nishizawa Masatoyo , Watanabe Hirohisa , Ito Mizuki , Sobue Gen , Ishikawa Kinya , Mizusawa Hidehiro , Kanai Kazuaki , Kuwabara Satoshi , Arai Kimihito , Koyano Shigeru , Kuroiwa Yoshiyuki , Hasegawa Kazuko , Yuasa Tatsuhiko , Yasui Kenichi , Nakashima Kenji , Ito Hijiri , Izumi Yuishin , Kaji Ryuji , kato Takeo , Kusunoki Susumu , Osaki Yasushi , Horiuchi Masahiro , Yamamoto Ken , Shimada Mihoko , Miyagawa Taku , Kawai Yosuke , Nishida Nao , Tokunaga Katsushi , Durr Alexandra , Brice Alexis , Filla Alessandro , Klockgether Thomas , Wuellner Ullrich , Tanner Caroline , Kukull Walter , Lee Virginia , Masliah Eliezer , Low Phillip , Sandroni Paola , Ozelius Laurie , Foroud Tatiana , Farrer Matthew , Trojanowski John 
Published: February 2024

Abstract
To elucidate the molecular basis of multiple system atrophy (MSA), a neurodegenerative disease, we conducted a genome-wide association study (GWAS) in a Japanese MSA case/control series followed by replication studies in Japanese, Korean, Chinese, European, and North American samples. In the GWAS stage, rs2303744 on chromosome 19 showed a suggestive association (P = 6.5 × 10− 7) that was replicated in additional Japanese samples (P = 2.9 × 10− 6, odds ratio (OR) = 1.58; 95% confidence interval (CI), 1.30–1.91), and then confirmed as highly significant in a meta-analysis of East Asian population data (P = 5.0 × 10− 15, OR = 1.49; 95% CI 1.35–1.65). The association of rs2303744 with MSA remained significant in combined European/North American samples (P = 0.017, OR = 1.12; 95% CI 1.02–1.23) despite allele frequencies being quite different between these populations. rs2303744 leads to an amino acid substitution in PLA2G4C that encodes the cPLA2γ lysophospholipase/transacylase. The cPLA2γ-Ile143 isoform encoded by the MSA risk allele has significantly decreased transacylase activity compared with the alternate cPLA2γ-Val143 isoform, and may perturb membrane phospholipids and promote oligodendroglial pathology.
Investigations of FFF Process Parameters for Printing UHMWPE / HAP + TiO2 Filament Prepared by A Developed Small-Scale Filament Extruder for Used in Biomedical Applications
Authors: Salama Ahmed , Osman T.A. , Rashad R. M. , Kamel Bahaa M. 
Published: February 2024

Abstract
The first aim of this work is to produce a small-scale filament extruder. The produced filament should be suitable for Fused Deposition Modeling (FDM) 3D printers. The filament production is not common and only made by several manufacturers around the world. The cheapest filament extruder machine on the market is still expensive compared to the 3D printer itself. Specifically, this paper describes the design, working principle and structure of a compacted thermo-plastic extrusion machine. One of the additive manufacturing processes used for the manufacture of functional and nonfunctional prototypes is fused filament fabrication (FFF), also known as freeform filament fabrication. FFF process settings have been shown to have a considerable impact on the mechanical, thermal, surface, morphological, and tribological properties of 3D printed objects in earlier research. The second aim of this research is to investigate the FFF process parameters for printing UHMWPE / HAP + TiO2 composite filament. Four main process parameters for the FFF process were adjusted in this study: infill %, bed temperature, extruder temperature, and outer perimeter. The ultimate tensile strength of the 3D printed UHMWPE / HAP + TiO2 prototypes (according to ASTM 638 type IV) was investigated using a universal tensile tester. The study's findings imply that the ultimate tensile strength can be maximized with a 100% infill percentage, 60OC bed temperature, 210 OC extruding temperature, and 5 outer perimeters. The other goal of this study is to replace the filament extrusion head of the 3D printer with a single screw extruder for printing the composite particle directly without the filament processing. Finally use the optimized parameter to print the same prototype directly and compare the results.
Photosynthesis and stress response of coal fly ash on stem elongation in wheat
Authors: Wang Shengpu , Hu Xinpeng , Li Bingbing , Zhang Haojia , Xiao Xin , Qian Ruoxi , Huang Xi 
Published: February 2024

Abstract

Coal is one of the primary energy sources in China and is widely used for electricity generation. Crops growing in overlapped areas of farmland and coal resources (OAFCR) suffer from coal fly ash stress, especially during stem elongation, which is a key stage that impacts wheat yield and is sensitive to environmental stress. As a primary food crop of China, wheat is essential for food security. However, the characteristics of wheat under the combined stress of fly ash and various heavy metals have not been sufficiently investigated. In this study, we explored the response of stem elongation in wheat to different levels of coal fly ash stress and determined the content of heavy metals (HMs) in wheat leaves. We found that with an increase in fly ash content, the Cu content in the shoots increased, while that in the roots decreased. Coal fly ash exposure reduced the proportions of Pb and Zn in the cytoderm, and the proportion of Cu in the soluble constituents decreased from 58.3–45.7%. Total chlorophyll, chlorophyll a, and chlorophyll b levels decreased significantly, whereas peroxidase (POD) and catalase (CAT) activities generally increased with increasing fly ash dose. Meanwhile, chloroplasts, mitochondria, and their internal structures were damaged, and the cell structures of leaves, such as the internal membrane structure, were damaged.
To BEV or not to BEV? A Comparative Life Cycle Assessment of Light-Duty Vehicles
Authors: Chen Hao , Sener Serife , Emburg Cole , Jones Miles , Bogucki Taylor , Bonilla Nicolas , Ijeoma Muzan , Wan Heng , Carbajales-Dale Michael 
Published: February 2024

Abstract

Electric vehicles (EVs) are promoted as ‘clean’ technologies and offer promising reductions in transportation emissions. Nevertheless, their environmental benefits critically depend on the local electricity grid mix and the type of emission being considered. Here, we conduct a comparative life cycle assessment (LCA) of the four dominant light-duty vehicles (LDVs) categories at both the global scale and across three prominent countries leading the global EV market – Norway, the US, and China – each with a distinct electricity grid mix. By analyzing different environmental indicators, specifically emphasizing global warming potential and respiratory effects, and quantifying related parametric uncertainties (e.g. fuel economy, electric driving share and vehicle lifetime mileage), we reveal that the EV advantages vary across these regions and across different types of environmental impacts. Our results support stakeholders in finding the most environmentally friendly vehicle and policy interventions while considering multiple factors, emphasizing tailored approaches over one-size-fits-all solutions in sustainable transportation.
Trametinib in the treatment of advanced lung adenocarcinoma with KRAS G12D mutation: a case report and literature review
Authors: Wang Jiacheng , Shen Hongchang , Jiang Yuanzhu , Meng Junzheng 
Published: February 2024

Abstract
The lung cancer poses a great threat to the patients’ health and social activities. Luckily, epithelial growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have greatly improved the response of patients getting EGFR-mutated non-small cell lung cancer (NSCLC), but the targeted therapy of KRAS mutations in lung cancer is still being explored; Currently, the effectiveness of drugs for KRAS mutations NSCLC is unsatisfactory. In this case, we present a advanced NSCLC with KRAS G12D mutation successfully treated with single-agent trametinib therapy. A 65-year-old non-smoking woman was diagnosed with stage IV lung adenocarcinoma after a lung CT for coughing revealed a lung lesion. She underwent next-generation sequencing (NGS) and revealed the KRAS G12D mutation. The single-agent target therapy of trametinib showed clinical benefit without obvious toxicity. This successful case of trametinib in advanced lung adenocarcinoma patients with KRAS G12D mutation suggests that trametinib may provide longer survival time for such patients. At the same time, we briefly reviewed the research progress of KRAS in NSCLC, and proposed possible challenges in the future.
Dynamic Knowledge Expansion: Real-time Text Classification with Deep Convolutional Neural Networks
Authors: Ferjani Imen , Hidri Minyar Sassi , Frihida Ali 
Published: February 2024

Abstract
Convolutional Neural Networks (CNNs) have shown exceptional prowess in text classification in the dynamic landscape of Natural Language Processing (NLP). It is difficult, however, to adapt to evolving concepts when overwhelmed with streaming data and retraining is cumbersome. We present an incremental learning solution tailored for real-world, computationally intensive applications in this paper. Using dynamic updates, we introduce a new taxonomy for text data stream classification that addresses the formidable challenge of concept drift. Using a modest dataset, we train a lean CNN. Using transfer learning, this model intelligently gleans insights from the data stream, leveraging knowledge gained from previous datasets. In order to classify data streams in real time, an online learning mechanism is deployed. A pretrained CNN is updated periodically to capture emerging features, accelerating learning even further. Our experimental results demonstrate the superiority of our approach, setting new benchmarks in performance. Our method allows NLP systems to remain agile, efficient, and state-ofthe- art in an era of relentless data flow.