bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conception and pregnancy among women with a live birth after breast cancer treatment: A survey study of young breast cancer survivors
Authors: Sorouri Kimia , Sella Tal , Rosenberg Shoshana M. , Loucks Maggie , Kirkner Gregory , Snow Craig , Ruddy Kathryn J. , Gelber Shari I. , Tamimi Rulla M. , Peppercorn Jeffrey M. , Schapira Lidia , Borges Virginia F. , Come Steven E. , Warner Ellen , Partridge Ann H. 
Published: October 2023

AbstractBackgroundBreast cancer (BC) is the most common malignancy in women of reproductive age. This study sought to explore the postcancer conception and pregnancy experience of young BC survivors to inform counseling.MethodsIn the Young Women's Breast Cancer Study (NCT01468246), a multicenter, prospective cohort, participants diagnosed at age ≤40 years with stage 0‐III BC who reported ≥1 postdiagnosis live birth were sent an investigator‐developed survey.ResultsOf 119 eligible women, 94 (79%) completed the survey. Median age at diagnosis was 32 years (range, 17–40) and at first postdiagnosis delivery was 38 years (range, 29–47). Most had stage I or II (77%) and HR+ (78%) BC; 51% were nulligravida at diagnosis. After BC treatment, most (62%) conceived naturally, though 38% used assisted reproductive technology, 74% of whom first attempted natural conception for a median of 9 months (range, 2–48). Among women with a known inherited pathogenic variant (n = 20), two underwent preimplantation genetic testing. Of 59 women on endocrine therapy before pregnancy, 26% did not resume treatment. Hypertensive disorders of pregnancy (20%) was the most common obstetrical condition. Nine percent of newborns required neonatal intensive care unit admission and 9% had low birth weight.ConclusionAmong women with live births after BC treatment, most conceived naturally and having a history of BC did not appear to negatively impact pregnancy complications, though the high rate of hypertensive disorders of pregnancy warrants further investigation. The prolonged period of attempting natural conception for some survivors suggests the potential need for improved understanding and counseling surrounding family planning goals after BC.
A Convenient Self‐Removing Affinity Tag Method for the Simple Purification of Tagless Recombinant Proteins
Authors: Prabhala Sai Vivek , Mayone Sophia A. , Moody Nathan M. , Kanu Chidinma B. , Wood David W. 
Published: October 2023

AbstractIn this work, we describe a novel self‐cleaving affinity tag technology based on a highly modified split‐intein cleaving element. In this system, which has recently been commercialized by Protein Capture Science, LLC under the name iCapTagTM, the N‐terminal segment of an engineered split intein is covalently immobilized onto a capture resin, while the smaller C‐terminal intein segment is fused to the N‐terminus of the desired target protein. The tagged target can then be expressed in an appropriate expression system, without concern for premature intein cleaving. During the purification, strong binding between the intein segments effectively captures the tagged target onto the capture resin while simultaneously generating a cleaving‐competent intein complex. After unwanted impurities are washed from the resin, cleavage of the target protein is initiated by a shift of the buffer pH from 8.5 to 6.2. As a result, the highly purified tagless target protein is released from the column in the elution step. Alternately, the resin beads can be added directly to cell culture broth or lysate, allowing capture, purification and cleavage of the tagless target protein using a column‐free format. These methods result in highly pure tagless target protein in a single step, and can thereby accelerate characterization and functional studies. In this work we demonstrate the single step purification of streptokinase, a fibrinolytic agent, and an engineered recombinant human hemoglobin 1.1 (rHb1.1). © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Expression of high‐titer protein tagged with the Nostoc punctiforme (Npu) DnaE split‐intein on the N‐terminusBasic Protocol 2: Purification of high‐titer protein using the Nostoc punctiforme (Npu) DnaE split‐intein purification platformAlternate Protocol 1: Expression of low‐titer protein tagged with the Nostoc punctiforme (Npu) DnaE split‐intein on the N‐terminusAlternate Protocol 2: Purification of low‐titer protein using the Nostoc punctiforme (Npu) DnaE split‐intein purification platform
Spatial Single Cell Analysis of Proteins in 2D Human Gastruloids Using Iterative Immunofluorescence
Authors: Freeburne Emily , Teague Seth , Khan Hina , Li Bolin , Ding Siyuan , Chen Bohan , Helms Adam , Heemskerk Idse 
Published: October 2023

AbstractDuring development, cell signaling instructs tissue patterning, the process by which initially identical cells give rise to spatially organized structures consisting of different cell types. How multiple signals combinatorially instruct fate in space and time remains poorly understood. Simultaneous measurement of signaling activity through multiple signaling pathways and of the cell fates they control is critical to addressing this problem. Here we describe an iterative immunofluorescence protocol and computational pipeline to interrogate pattern formation in a 2D model of human gastrulation with far greater multiplexing than is possible with standard immunofluorescence techniques. This protocol and computational pipeline together enable imaging followed by spatial and co‐localization analysis of over 27 proteins in the same gastruloids. We demonstrate this by clustering single cell protein expression, using techniques familiar from scRNA‐seq, and linking this to spatial position to calculate spatial distributions and cell signaling activity of different cell types. These methods are not limited to patterning in 2D gastruloids and can be easily extended to other contexts. In addition to the iterative immunofluorescence protocol and analysis pipeline, Support Protocols for 2D gastruloid differentiation and producing micropatterned multi‐well slides are included. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Iterative immunofluorescenceBasic Protocol 2: Computational analysis pipelineSupport Protocol 1: Generating micropatterned multi‐well slidesSupport Protocol 2: Differentiation of 2D gastruloids
Synthetic polyploidy in spice crops: A review
Authors: Neenu Maria George , Aswathi Akkaraparambil , Prasath Duraisamy 
Published: October 2023

AbstractSpice crops comprise diverse, economically valuable crops with numerous applications ranging from culinary to pharmaceutical industries. Spices are an integral part of cuisines worldwide, imparting characteristic flavor, aroma, and pungency to food. Breeding and crop improvement efforts in spices have focused on enhancing yield and quality parameters (essential oil, oleoresin, fiber, etc.) along with bioactive chemical constituents. The prominent breeding strategies followed are selection, hybridization, mutation, in vitro approaches, and transgenics. Polyploidy is one of the drivers of speciation and evolution, increasing the biological diversity in many crops, including spices. Polyploidy, either through natural means or artificial induction, broadens the scope of crop improvement. The artificial induction of polyploidy is usually done via antimitotic chemicals, duplicating the complete chromosomal set and allowing for genetic alterations and rearrangements that result in phenotypic changes across the board. As a result, increasing ploidy in crops often results in improved yield, biomass, vigor, biotic and abiotic stress tolerance, and secondary metabolite production, all of which can contribute to the economic success of these crops. This review provides an overview of research on artificial polyploidization in spice crops, including the polyploidy induction system, polyploidy generation, and screening methods to select the polyploids of interest. Thus, we have summarized the significant applications of artificial polyploidy in crop improvement that can serve as a potent reference for future research works in the same direction in the under‐explored spice crops.
A novel MIMO SMC for comprehensive unified control of the cascaded DC‐DC and DC‐AC converters in grid connected photovoltaic systems
Authors: Radmand Fereshteh , Hosseini Firouz Mansour , Salimi Mahdi , Babaei Ebrahim 
Published: October 2023

SummaryIt is well known that DC‐DC boost converters are cascaded with DC‐AC inverters for grid connection of the photovoltaic (PV) systems. In the traditional control approaches, the mentioned DC‐DC and DC‐AC converters are controlled separately to facilitate the controller design problem. However, from the controller design viewpoint, the overall structure of the grid connected PV generator is a multi‐input multi‐output (MIMO) system. The duty cycle of the DC‐DC converter and inverter modulation index are the control inputs and, on the other hand, generated photovoltaic DC power and exported power to the grid are control outputs. Moreover, the inverter DC link voltage should be stabilized by the closed‐loop controller as well as an internal control output. If controllers are designed separately, it means that the interaction between DC‐DC and DC‐AC controllers isn't considered accurately, and since the isolated models of DC‐DC converter and DC‐AC inverter are extracted based on some approximated assumptions, separate controller design cannot guarantee stability and robustness of the whole system in a wide range of operation. To cope with these problems, in this paper, a novel MIMO sliding mode controller (SMC) is developed for comprehensive closed‐loop control of the DC‐DC boost converter cascaded with a single‐phase DC‐AC grid connected photovoltaic inverter. In the proposed approach, the dynamic model of whole system is developed comprehensively at first, and then, a unique MIMO controller is designed to control both DC‐to‐AC and DC‐to‐DC converters together. To cope with the nonlinear characteristic of the system and uncertainty of model parameters in a wide range, a fixed‐frequency SMC is developed using the comprehensive state space model of the closed loop system. In the proposed MIMO‐SMC controller, the AC power (which is exported to the grid) and operating point of the PV source are controlled via inverter modulation index and duty cycle of the DC‐DC boost converter, respectively. Another major advantage of the proposed system is mitigating the non‐minimum phase characteristic of the boost converter through the indirect control of inverter DC link capacitor. To evaluate the performance of the designed control system, simulation results are compared with a standard linear PI controller. It is shown that the developed system has zero steady‐state error and enjoys faster dynamic response during the start‐up and step changes of AC and DC current references. Moreover, it can maintain the stability of closed‐loop systems in a wide range of operations.
PCA‐based fast point feature histogram simplification algorithm for point clouds
Authors: Gan Zhong , Ma Boyu , Ling Zihao 
Published: October 2023

AbstractIn order to realize efficient and lightweight digital inspection technology, we propose an improved method to simplification large volumes of scanned point cloud data of aircraft integral panels and facilitate subsequent processing. Fast Point Feature Histogram (FPFH) method is utilized to extract feature information and optimize the Principal Component Analysis (PCA) algorithm to calculate the contribution degree to transform into principal components, yielding PCA‐based FPFH features. Next, based on PCA‐based FPFH features, we classify the point cloud data into non‐feature and feature point clouds and extract the feature point clouds through random downsampling to obtain simplified non‐feature point clouds, extract the feature point clouds to retain their boundary integrity, and downsample the remaining feature point clouds by curvature to obtain the simplified feature point clouds. Finally, we combine the two to obtain the final simplified panel point cloud data within 30 s. To evaluate the simplification effect, we adopt a standardized information entropy‐based point cloud simplification accuracy evaluation method based on the simplification rate. Our method achieves an information entropy of more than 0.95, indicating its effectiveness in simplification point cloud data for efficient and lightweight digital inspection technology.
Metabolic connectivity as a predictor of surgical outcome in mesial temporal lobe epilepsy
Authors: Strýček Ondřej , Říha Pavel , Kojan Martin , Řehák Zdeněk , Brázdil Milan 
Published: October 2023

AbstractObjectiveThe study investigated metabolic connectivity (MC) differences between patients with unilateral drug‐resistant mesial temporal lobe epilepsy (MTLE) with hippocampal sclerosis (HS) and healthy controls (HCs), based on [18 F]‐fluorodeoxyglucose (FDG)–PET data. We focused on the MC changes dependent on the lateralization of the epileptogenic lobe and on correlations with postoperative outcomes.MethodsFDG–PET scans of 47 patients with unilateral MTLE with histopathologically proven HS and 25 HC were included in the study. All the patients underwent a standard anterior temporal lobectomy and were more than 2 years after the surgery. MC changes were compared between the two HS groups (left HS, right HS) and HC. Differences between the metabolic network of seizure‐free and non‐seizure‐free patients after surgery were depicted afterward. Network changes were correlated with clinical characteristics.ResultsThe study showed widespread metabolic network changes in the HS patients as compared to HC. The changes were more extensive in the right HS than in the left HS. Unfavorable surgical outcomes were found in patients with decreased MC within the network including both the lesional and contralesional hippocampus, ipsilesional frontal operculum, and contralesional insula. Favorable outcomes correlated with decreased MC within the network involving both orbitofrontal cortices and the ipsilesional temporal lobe.SignificanceThere are major differences in the metabolic networks of left and right HS, with more extensive changes in right HS. The changes within the metabolic network could help predict surgical outcomes in patients with HS. MC may identify patients with potentially unfavorable outcomes and direct them to a more detailed presurgical evaluation.Plain Language SummaryMetabolic connectivity is a promising method for metabolic network mapping. Metabolic networks in mesial temporal lobe epilepsy are dependent on lateralization of the epileptogenic lobe and could predict surgical outcomes.
Cyperus pangorei porous carbon electrodes for the sustainable symmetric supercapacitor application
Authors: Priya D. Siva , Kennedy L. John , Anand G. Theophil 
Published: October 2023

AbstractEfficiently addressing natural biowaste has the potential to effectively mitigate pressing environmental concerns. Given the growing demand for energy, it is imperative to identify a safe and environmentally friendly method for producing biowaste‐based porous carbon (PC). Biowaste serves valuable carbon sources for the cost‐effective production of efficient PC materials. In this study, we focus on ongoing research aimed at converting a small‐scale industrial waste of Cyperus pangorei, into PC. To achieve this, we employed a simple and eco‐friendly approach, optimizing the process at four different temperatures to enhance its suitability for supercapacitor applications. By subjecting the Cyperus pangorei waste to a two‐step chemical activation process using potassium hydroxide (KOH) at 800°C (referred to as CPC8), we obtained a PCs with a well‐defined pore matrix, exhibiting a surface area of 711.2 m2g−1. The resulting PC exhibited 194.6 Fg−1 (0.5 Ag−1) of specific capacitance when tested in a 1 M Na2SO4 aqueous electrolyte. The galvanostatic study demonstrated remarkable 88.5 Whkg−1 of energy density at 1.7 kWkg−1 power density, along with a capacitance retention rate of 97.9% at 5000th cycle (8 Ag−1). Furthermore, electrochemical impedance study revealed 0.053 Ω of internal resistance, indicating enhanced ionic diffusion. Based on the excellent performance characteristics exhibited by CPC8, its practical application in supercapacitors holds great promise.
Beamforming and data detection in intelligent reflecting surfaces‐assisted communication using deep learning
Authors: Raghavendra Ashwini H. , Gurugopinath Sanjeev 
Published: October 2023

SummaryIn this article, we propose two deep neural network (DNN) architectures for an intelligent reflecting surface (IRS)‐assisted network with a single transmitter and multiple receivers. The first DNN is designed to obtain an optimal beamforming weight vector that maximizes the achievable sum rate over the IRS‐aided network. The beamforming vector is designed based on a pilot‐based channel estimation procedure, which requires only a small fraction of the available number of reflecting surfaces on the IRS to be active. The obtained vector is fed to the second DNN, which is designed for detecting information symbols from the transmitter. Our simulation study shows that the achievable rate obtained using the designed beamforming vector approaches the maximum achievable rate quickly, as the number of active elements increases. The impact of the number of active elements and the beamforming vector on the performance of the detector is also studied, in terms of the bit error rate (BER). We show that the BER performance of the second DNN is close to that of the optimal maximum likelihood detector. Further, we study the effect of the channel estimation errors on the performance of the DNNs. Moreover, we study the effect of the hyperparameters of the architectures on the training time and performance, in terms of root mean squared error and accuracy.
Physicochemical properties and survival assessment of potential probiotics in a novel dairy drink during storage
Authors: Gedik Oğuzhan , Karahan Aynur Gül 
Published: October 2023

AbstractA novel fermented dairy drink utilizing microbial strains displaying potential probiotic attributes was formulated. The study constituted several experimental cohorts, including Lactiplantibacillus plantarum AB6‐25, isolated from a human stool sample; Lacticaseibacillus casei K2, sourced from a koumiss sample; Lacticaseibacillus rhamnosus 3B7, derived from a traditional yogurt sample; and identical lactic acid bacteria (LAB) in combination with yeast (Saccharomyces boulardii T8‐3C from chicken feces) strains. Two distinct iterations of probiotic‐fermented dairy drinks were generated by introducing subcultured microorganism cultures: LAB strains at a concentration of 1% each (designated as combination A) and a blend of LAB strains at 1% each along with T8‐3C at 0.5% (designated as combination B) into both whole and semi‐skimmed milk matrices. The fermentation process persisted until the pH reached 4.6 under constant conditions of 37 ± 1°C. Subsequently, the samples were held at 4 ± 1°C for 15 days. The groups' physicochemical, microbiological, and sensory characteristics were determined on days 1, 8, and 15 of storage, and the protein profile was determined. Standardized regression analysis and principal component analysis evaluated the results. Fat content affected the changes in dry matter. pH decreased in all samples during storage, particularly in the yeast group. The microorganism group positively affected syneresis, whereas fat content and the interaction of fat content and the microorganism group had a negative effect. The most critical factor in the decrease in syneresis was the increase in fat content. LAB and yeasts maintained their probiotic effects during storage, with a viability level of approximately 109 and 108 cfu/mL, respectively.
Investigating salivary concentration of calcium ion in hypothyroidism
Authors: Borzuei Shiva , Amjad Samaneh Vaziri , Badiei Mohammadreza , Farmany Abbas , Cheraghi Zahra 
Published: October 2023

AbstractBackground and AimsHypothyroidism is the most common disease of the thyroid gland. Thyroid hormone plays a crucial role in regulating tissue growth and metabolism. Additionally, patients with thyroid dysfunction have a higher incidence of caries and periodontal problems. The presence of calcium and phosphate ions seems to be effective in remineralizing teeth and reducing caries. The aim of this study was to evaluate the salivary level of calcium in patients with hypothyroidism.ResultsThe results of this study showed that saliva calcium concentration in patients with hypothyroidism is significantly lower than healthy subjects. Also, no significant association was found between salivary calcium levels and TSH and T4 hormones level of blood.ConclusionThe increased prevalence of dental caries in patients with hypothyroidism may be related to a decrease in their calcium levels.
The role of the multidisciplinary team in surgical management of intractable tubo‐ovarian abscess as a late sequelae of challenging Crohn's disease in the modern era: A case report and review of current literature
Authors: Loganathan Shanghavie , Smyth Sarah Louise , Mykula Roman , Soleymani Majd Hooman 
Published: October 2023

AbstractChronic, severe Crohn's disease in a young female patient can result in surgical complexity. The rarity of the presentation of intractable pelvic abscesses within this etiology with additional considerations given to fertility concerns and hence requirement for input from a multi‐disciplinary team makes this a vital case in building a consensus for evidence‐based surgical management. A 29‐year‐old nulliparous woman was referred to our tertiary centre for surgical management of Crohn's disease with known tubo‐ovarian abscess and abdominoperineal and abdominal wall sinuses. Her previous surgical history included 4 midline laparotomies, subtotal colectomy and proctectomy with stoma formation. The patient underwent egg collection to preserve fertility. This was followed by midline laparotomy and abdominoperineal resection, which involved a retrograde radical modified hysterectomy using the Hudson technique, alongside excision of the perineal sinus, with reconstruction of the perineal defect using an internal pudendal artery perforator gluteal fold flap, and in addition to excision and drainage of the abdominal wall abscess. Involvement was sought from gynecological oncology, colorectal, urology, plastics, stoma, fertility, microbiology, and gastroenterology teams, which enabled successful preservation of end organ function and improvement in patient psychological well‐being. This case is a paradigm of surgical challenge, requiring expert gynecological oncology techniques including a retroperitoneal approach, nerve and vessel sparing considerations alongside colorectal and urological procedures. Moreover, we believe that our blueprint for effective multi‐disciplinary practice will inform the future management of gynecological surgery. Therefore this report aims to contribute towards the optimum management of the gynecological sequelae of Crohn's disease.