bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reliability and Validity of the Japanese Version of the Survey of Perceived Organizational Support
Authors: Odagami Kiminori , Nagata Tomohisa , Eguchi Hisashi , Inoue Akiomi , Mafune Kosuke , Mori Koji 
Published: November 2023

ABSTRACTObjectivesThis study aimed to validate the Japanese version of the Survey of Perceived Organizational Support (SPOS-J) for the Japanese workforce by examining its reliability, factorial, construct, and test-retest validity.MethodsWe conducted an online baseline survey with 6,220 employed Japanese individuals, followed by a follow-up survey two weeks later to examine the test-retest reliability of the SPOS-J. The SPOS-J was translated in accordance with the ISPOR Task Force Guidelines. Both confirmatory and exploratory factor analyses were utilized to examine factorial validity. Construct validity was assessed using Pearson’s correlation analysis, while internal consistency was determined through the calculation of Cronbach’s alpha values. Additionally, test-retest reliability was evaluated with Cohen’s weighted kappa and intraclass correlation coefficients.ResultsThe SPOS-J demonstrated a better fit for a two-factor structure, comprising two subscales - SPOS-J (supported) and SPOS-J (unsupported) – compared to the one-factor structure of the original version. It exhibited high internal consistency, with Cronbach’s alpha coefficients of .93 for the overall SPOS-J, .97 for the SPOS-J (supported), and .95 for the SPOS-J (unsupported). Test-retest reliability was found to be good for the overall SPOS-J (.62) and moderate for its subscales, with Cohen’s weighted kappa values of .54 for SPOS-J (supported) and .42 for SPOS-J (unsupported). The construct validity was confirmed with significant correlations between the SPOS-J, its antecedents, and outcomes.ConclusionsThe SPOS-J is a reliable and valid measure for assessing perceived organizational support among Japanese workers.
Creation of de novo cryptic splicing for ALS/FTD precision medicine
Authors: Wilkins Oscar G. , Chien Max Z.Y.J. , Wlaschin Josette J. , Pisliakova Maria , Thompson David , Digby Holly , Simkin Rebecca L. , Diaz Juan Antinao , Mehta Puja R. , Keuss Matthew J. , Zanovello Matteo , Brown Anna-Leigh , Harley Peter , Darbey Annalucia , Karda Rajvinder , Fisher Elizabeth M.C. , Cunningham Tom J. , Le Pichon Claire E. , Ule Jernej , Fratta Pietro 
Published: November 2023

AbstractA system enabling the expression of therapeutic proteins specifically in diseased cells would be transformative, providing greatly increased safety and the possibility of pre-emptive treatment. Here we describe “TDP-REG”, a precision medicine approach primarily for amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), which exploits the cryptic splicing events that occur in cells with TDP-43 loss-of-function (TDP-LOF) in order to drive expression specifically in diseased cells. In addition to modifying existing cryptic exons for this purpose, we develop a deep-learning-powered algorithm for generating customisable cryptic splicing events, which can be embedded within virtually any coding sequence. By placing part of a coding sequence within a novel cryptic exon, we tightly couple protein expression to TDP-LOF. Protein expression is activated by TDP-LOFin vitroandin vivo, including TDP-LOF induced by cytoplasmic TDP-43 aggregation. In addition to generating a variety of fluorescent and luminescent reporters, we use this system to perform TDP-LOF-dependent genomic prime editing to ablate theUNC13Acryptic donor splice site. Furthermore, we design a panel of tightly gated, autoregulating vectors encoding a TDP-43/Raver1 fusion protein, which rescue key pathological cryptic splicing events. In summary, we combine deep-learning and rational design to create sophisticated splicing sensors, resulting in a platform that provides far safer therapeutics for neurodegeneration, potentially even enabling preemptive treatment of at-risk individuals.One-Sentence SummaryWe engineer TDP-43-regulated cryptic exons, enabling exceptionally precise activation of gene therapies in diseased neurons.
Illuminating the complete ß-cell mass of the human pancreas - signifying a new view on the islets of Langerhans
Authors: Lehrstrand Joakim , Davies Wayne I. L. , Hahn Max , Korsgren Olle , Alanentalo Tomas , Ahlgren Ulf 
Published: November 2023

ABSTRACTPancreatic islets of Langerhans play a pivotal role in regulating blood glucose homeostasis, but critical information regarding their mass, distribution and composition is lacking within a whole organ context. Here, a new 3D imaging pipeline was applied to generate a first complete account of the insulin-producing islets throughout the human pancreas at a microscopic resolution and within a maintained spatial 3D context. These data show that human islets are far more heterogenous than previously accounted for with regards to their size distribution and cellular make up. By deep tissue 3D imaging, this in-depth study demonstrates that 50% of the human insulin-expressing islets are virtually devoid of glucagon-producing a-cells, an observation with significant implications for both experimental and clinical research.One Sentence Summary:New islet heterogeneity identified in the human pancreas
Fragment-based screening targeting an open form of the SARS-CoV-2 main protease binding pocket
Authors: Huang Chia-Ying , Metz Alexander , Lange Roland , Artico Nadia , Potot Céline , Hazemann Julien , Müller Manon , Santos Marina Dos , Chambovey Alain , Ritz Daniel , Eris Deniz , Meyer Solange , Bourquin Geoffroy , Sharpe May , Sweeney Aengus Mac 
Published: November 2023

AbstractTo identify starting points for therapeutics targeting SARS-CoV-2, the Paul Scherrer Institute and Idorsia decided to collaboratively perform an X-ray crystallographic fragment screen against its main protease. Fragment-based screening was carried out using crystals with a pronounced open conformation of the substrate binding pocket. Of 631 fragments soaked, a total of 29 hits bound either in the active site (24 hits), a remote binding pocket (2 hits) or at crystal packing interfaces (3 hits). Notably, two fragments with a pose sterically incompatible with a more occluded crystal form were identified. Two isatin-based electrophilic fragments bound covalently to the catalytic cysteine residue. Our structures also revealed a surprisingly strong influence of the crystal form on the binding pose of three published fragments used as positive controls, with implications for fragment screening by crystallography.SynopsisAn X-ray crystallographic screen on SARS-CoV-2 3CL protease resulted in 29 fragment hits, including two isatin-based reversible covalent binders, and revealed a strong influence of the crystal form used for fragment soaking on the bound conformation of three additional reference fragments.
TheHansenula polymorphaMitochondrial Carrier Family proteins Mir1 and Aac2 are dually localized at peroxisomes and mitochondria
Authors: Pedersen Marc Pilegaard , Wolters Justina C. , de Boer Rinse , Krikken Arjen M. , van der Klei Ida J. 
Published: November 2023

AbstractPeroxisomes are ubiquitous cell organelles involved in various metabolic pathways. In order to properly function, several cofactors, substrates and products of peroxisomal enzymes need to pass the organellar membrane. So far only a few transporter proteins have been identified. We analysed peroxisomal membrane fractions purified from the yeastHansenula polymorphaby untargeted label-free quantitation mass spectrometry. As expected, several known peroxisome-associated proteins were enriched in the peroxisomal membrane fraction. In addition, several other proteins were enriched, including mitochondrial transport proteins. Localization studies revealed that two of them, the mitochondrial carrier family proteins Aac2 and Mir1, have a dual localization on mitochondria and peroxisomes. To better understand the molecular mechanisms of dual sorting, we tested the localization of Mir1 in cells lacking Pex3 or Pex19, two peroxins that play a role in targeting of peroxisomal membrane proteins. In these cells Mir1 only localized to mitochondria, indicating that Pex3 and Pex19 are required to sort Mir1 to peroxisomes. Analysis of the localization of various truncated versions of Mir1 in wild-typeH. polymorphacells revealed that several localized to mitochondria, but only one, consisting of the transmembrane domains 3-6, was peroxisomal. Peroxisomal localization of this construct was lost in aMIR1deletion strain, indicating that full length Mir1 was required for the localization of the truncated protein to peroxisomes. Our data suggest that only full length Mir1 sorts to peroxisomes, while Mir1 contains multiple regions with mitochondrial sorting information.
Structuring effects of archaeal replication origins
Authors: Mottez Clémence , Puech Romain , Flament Didier , Myllykallio Hannu 
Published: November 2023

AbstractArchaea use eukaryotic-like DNA replication proteins to duplicate circular chromosomes similar to those of bacteria. Although archaeal replication origins have been maintained during the evolution, they are non-essential under laboratory conditions. Here we propose the local deviations from Chargaff’s second parity rule of archaeal chromosomes result from the biased gene orientation and not from mutational biases. Our computational and experimental analyses indicate that the archaeal replication origins prevent head-to-head collisions of replication and transcription complexes as well as participate in coordination of the transfer of genetic information. Our results therefore suggest that the archaeal replication origins have alternative functions not related to their role in initiation of DNA replication.
Pooled screening for biochemically-defined phenotypes using CRISPuRe-Seq
Authors: Harris David T. , Jan Calvin H. 
Published: November 2023

AbstractPhenotypic genetic screening is a powerful approach for biological discovery. CRISPR technologies have enabled routine genome-scale pooled perturbations in cultured cells, creating an opportunity to develop screening methods capable of accessing molecularly meaningful phenotypes. Here we present a generalized strategy, named CRISPuRe-seq, that enables pooled screening of a broad range of protein-based attributes. This approach utilizes the purification of an RNA-barcoded protein-of-interest to identify causative genetic perturbations. As a proof of concept, we screened for modifiers of Interferon alpha signaling and were able to identify all of the primary components of the type 1 Interferon JAK/STAT signaling pathway.
Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis
Authors: Kotb Noor M. , Ulukaya Gulay , Chavan Ankita , Nguyen Son C. , Proskauer Lydia , Joyce Eric , Hasson Dan , Jagannathan Madhav , Rangan Prashanth 
Published: November 2023

AbstractGenome organization can regulate gene expression and promote cell fate transitions. The differentiation of germline stem cells (GSCs) to oocytes in Drosophila involves changes in genome organization mediated by heterochromatin and the nuclear pore complex (NPC). Heterochromatin represses germ-cell genes during differentiation and NPCs anchor these silenced genes to the nuclear periphery, maintaining silencing to allow for oocyte development. Surprisingly, we find that genome organization also contributes to NPC formation, mediated by the transcription factor Stonewall (Stwl). As GSCs differentiate, Stwl accumulates at boundaries between silenced and active gene compartments. Stwl at these boundaries plays a pivotal role in transitioning germ-cell genes into a silenced state and activating a group of oocyte genes and Nucleoporins (Nups). The upregulation of these Nups during differentiation is crucial for NPC formation and further genome organization. Thus, crosstalk between genome architecture and NPCs is essential for successful cell fate transitions.
The mechanism of mRNA activation
Authors: Gentry Riley C. , Ide Nicholas A. , Comunale Victoria M. , Hartwick Erik W. , Kinz-Thompson Colin D. , Gonzalez Ruben L. 
Published: November 2023

SummaryDuring translation initiation, messenger RNA molecules must be identified and activated for loading into a ribosome. In this rate-limiting step, the heterotrimeric protein eukaryotic initiation factor eIF4F must recognize and productively interact with the 7-methylguanosine cap at the 5’ end of the messenger RNA and subsequently activate the message. Despite its fundamental, regulatory role in gene expression, the molecular events underlying cap recognition and messenger RNA activation remain mysterious. Here, we generate a unique, single-molecule fluorescence imaging system to interrogate the dynamics with which eIF4F discriminates productive and non-productive locations on full-length, native messenger RNA molecules. At the single-molecule level, we observe stochastic sampling of eIF4F along the length of the messenger RNA and identify allosteric communication between the eIF4F subunits which ultimately drive cap-recognition and subsequent activation of the message. Our experiments uncover novel functions for each subunit of eIF4F and we conclude by presenting a model for messenger RNA activation which precisely defines the composition of the activated message. This model provides a general framework for understanding how messenger RNA molecules may be discriminated from one another, and how other RNA-binding proteins may control the efficiency of translation initiation.
Fake news in business and management literature: a systematic review of definitions, theories, methods and implications
Authors: Farhoudinia Bahareh , Ozturkcan Selcen , Kasap Nihat 
Published: November 2023

PurposeThis paper aims to conduct an interdisciplinary systematic literature review (SLR) of fake news research and to advance the socio-technical understanding of digital information practices and platforms in business and management studies.Design/methodology/approachThe paper applies a focused, SLR method to analyze articles on fake news in business and management journals from 2010 to 2020.FindingsThe paper analyzes the definition, theoretical frameworks, methods and research gaps of fake news in the business and management domains. It also identifies some promising research opportunities for future scholars.Practical implicationsThe paper offers practical implications for various stakeholders who are affected by or involved in fake news dissemination, such as brands, consumers and policymakers. It provides recommendations to cope with the challenges and risks of fake news.Social implicationsThe paper discusses the social consequences and future threats of fake news, especially in relation to social networking and social media. It calls for more awareness and responsibility from online communities to prevent and combat fake news.Originality/valueThe paper contributes to the literature on information management by showing the importance and consequences of fake news sharing for societies. It is among the frontier systematic reviews in the field that covers studies from different disciplines and focuses on business and management studies.
Design of hexacopter and finite element analysis for material selection
Authors: Raut Rituraj , Jadhav Savitri , Jadhav Nathrao B. 
Published: November 2023

PurposeThe purpose of this study is to offer a better and more effective hexacopter design for a 3 kg payload using finite element analysis (FEA), facilitating the use of different materials for different components that too without compromising strength.Design/methodology/approachA 3D computer-aided design (CAD) model of a hexacopter with a regular hexagonal frame is presented. Furthermore, a finite element model is developed to perform a structural analysis and determine Von Mises stress and strain values along with deformations of different components of the proposed hexacopter design.FindingsThe results establish that carbon fibre outperforms acrylonitrile butadiene (ABS) with respect to deformations. Within the permissible limits of the stress and strain values, both carbon fiber and ABS are suggested for different components. Thus, a proposed hexacopter offers lighter weight, high strength and low cost.Originality/valueThe use of different materials for different components is suggested by making use of static structural analysis. This encourages new research work and helps in developing new applications of hexacopter, and it has never been reported in literature. The suggested materials for the components of the hexacopter will prove to be suitable considering weight, strength and cost.
Sharia governance and organizational performance in zakat management organization: evidence from Indonesia
Authors: Zakiy Faris Shalahuddin , Falikhatun Falikhatun , Fauziah Najim Nur 
Published: November 2023

Purpose
This paper aims to investigate the impact of sharia governance on organizational performance in zakat management institutions in Indonesia over the period 2017–2021.

Design/methodology/approach
This study examined 33 zakat management organizations in Indonesia from 2017 through 2021 for 151 observations. Gross allocation ratio and growth of ZIS collection are used as organizational performance measures. The independent variables in this study are board of director size, educational background of the board of directors, sharia supervisory board size, sharia supervisory expertise, supervisory size and management size. Also, the study uses size, age and audit opinion as control variables to help measure the relationship between sharia governance and organizational performance.

Findings
This study shows that the board of directors and supervisory size positively and significantly affect organizational performance. Then, the educational background of board of directors has a negative and significant effect on organizational performance. In Model 1, sharia supervisory board size has a positive and significant effect on organizational performance, but in Model 2, sharia supervisory board size does not. Meanwhile, sharia supervisory expertise and management board size do not affect organizational performance.

Practical implications
The findings in this study illustrate the importance of transparency in the zakat management organization. Transparency helps minimize conflicts of interest and information asymmetry in the zakat management organization. In addition, sharia governance mechanism helps regulators and top management to make effective policies to improve and enhance organizational performance.

Social implications
Sharia governance is essential for zakat management organizations to increase accountability, credibility and public trust and support the practice of zakat management organizations.

Originality/value
This study discusses sharia governance and organizational performance in socioreligious organizations, especially zakat management organizations, which are still rarely carried out. Thus, this study broadens the insights of sharia governance and highlights the importance of performance appraisal in zakat management organizations.
Is combatting money laundering an integrity issue? Insights from Nigeria
Authors: Umar Bello 
Published: November 2023

Purpose
This study aims to determine how integrity influences money laundering combatting.

Design/methodology/approach
A qualitative approach using methodological triangulation was used to answer the question to describe and understand the phenomena from the participants’ perspective. Data was gathered with a semi-structured questionnaire, observation and field notes.

Findings
Analysis revealed that 93% of law enforcement investigators believe integrity is required to combat money laundering. They also observed that integrity is needed for the political environment, institutions and their personnel or officers.

Practical implications
There is a need for integrity in the economy’s public and private sectors to combat money laundering effectively. Integrity must be present in the political environment, institutions and personnel. Hence, a recommendation is to appoint chief integrity officers in all stakeholder organisations.

Originality/value
This study is among the few research that covers the area of integrity and its influence on combatting money laundering from law enforcement investigators’ perspective.
Suppliers' GHG mitigation strategies (Scope 3): the case of a steelmaking company
Authors: Fagundes Alves Marino Yago , Marques Vieira Luciana , Beal Partyka Raul 
Published: November 2023

PurposeThe emission of greenhouse gases has become an increasingly relevant topic in supply chain management. The steel industry is a highly intensive manufacturing industry with significant emission levels, particularly Scope 3 emissions, which are the indirect emissions from suppliers. Since a supply chain is seen as a non-mandatory measurement item within GHG measurement protocols, this article contributes to the literature on assessing the suppliers of a focal company relative to their emissions for complying with Scope 3 (indirect emissions). It adds to the evolving literature on low-carbon supply chains.Design/methodology/approachThis study first conducted a survey with 110 suppliers from a focal transnational buyer company. A cluster analysis was performed, and ANOVA compared constructs relating to public or private ownership and country of origin. Finally, regression tested the relationship between the motivators and governance in the mitigation strategies.FindingsUsing cluster analysis, two groups of companies were found that have statistically significant differences. The influence of the country of origin was also found in relation to governance and mitigation strategies, as was the influence of the type of company on governance. Furthermore, the more motivated the suppliers and the more governance measures they adopt, the more companies adopt their own GHG mitigation strategies. These findings are summarized by way of an analytical framework that integrates the constructs with empirical evidence.Originality/valueThe steel industry is a sector that is particularly energy-intensive and produces millions of tons of CO2 per year. Emissions from its SC (Scope 3) are relevant but still seen as a non-mandatory item for measurement purposes within the GHG measurement protocols, which leads to less attention being paid to the subject. This study contributes by way of its analytical framework that is validated by empirical data that can be tested in further studies.