bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-alcoholic fatty liver disease biomarkers estimate cardiovascular risk based on coronary artery calcium score in type 2 diabetes: a cross-sectional study with two independent cohorts
Authors: Denimal Damien , Ponnaiah Maharajah , Jeannin Anne-Caroline , Phan Franck , Hartemann Agnès , Boussouar Samia , Charpentier Etienne , Redheuil Alban , Foufelle Fabienne , Bourron Olivier 
Published: February 2024

Abstract
Background
Studies have demonstrated that coronary artery calcification on one hand and non-alcoholic fatty liver disease (NAFLD) on the other hand are strongly associated with cardiovascular events. However, it remains unclear whether NAFLD biomarkers could help estimate cardiovascular risk in individuals with type 2 diabetes (T2D). The primary objective of the present study was to investigate whether the biomarkers of NAFLD included in the FibroMax® panels are associated with the degree of coronary artery calcification in patients with T2D.

Methods
A total of 157 and 460 patients with T2D were included from the DIACART and ACCoDiab cohorts, respectively. The coronary artery calcium score (CACS) was measured in both cohorts using computed tomography. FibroMax® panels (i.e., SteatoTest®, FibroTest®, NashTest®, and ActiTest®) were determined from blood samples as scores and stages in the DIACART cohort and as stages in the ACCoDiab cohort.

Results
CACS significantly increased with the FibroTest® stages in both the DIACART and ACCoDiab cohorts (p-value for trend = 0.0009 and 0.0001, respectively). In DIACART, the FibroTest® score was positively correlated with CACS in univariate analysis (r = 0.293, p = 0.0002) and remained associated with CACS independently of the traditional cardiovascular risk factors included in the SCORE2-Diabetes model [β = 941 ± 425 (estimate ± standard error), p = 0.028]. In the ACCoDiab cohort, the FibroTest® F3-F4 stage was positively correlated with CACS in point-biserial analysis (rpbi = 0.104, p = 0.024) and remained associated with CACS after adjustment for the traditional cardiovascular risk factors included in the SCORE2-Diabetes model (β = 234 ± 97, p = 0.016). Finally, the prediction of CACS was improved by adding FibroTest® to the traditional cardiovascular risk factors included in the SCORE2-Diabetes model (goodness-of-fit of prediction models multiplied by 4.1 and 6.7 in the DIACART and ACCoDiab cohorts, respectively). In contrast, no significant relationship was found between FibroMax® panels other than FibroTest® and CACS in either cohort.

Conclusions
FibroTest® is independently and positively associated with the degree of coronary artery calcification in patients with T2D, suggesting that FibroTest® could be a relevant biomarker of coronary calcification and cardiovascular risk.
Trial registration: ClinicalTrials.gov identifiers NCT02431234 and NCT03920683.
Advances in the assessment of cosmetic outcomes, sensory alteration in surgical areas, and health-related quality of life of endoscopic thyroidectomy
Authors: Zhou Di , Zhang Zeyu , Dou Xiaolin , Xia Fada , Li Xinying 
Published: February 2024

Abstract
Background
Endoscopic thyroidectomy has been preliminarily proven effective and safe for thyroid diseases. The cosmetic outcomes and life quality are critical contents of postoperative assessment. This review will primarily focus on the assessment methods and results related to cosmetic outcomes, sensory alteration of surgical area, and quality of life following endoscopic thyroidectomy.

Methods
A comprehensive search of published articles within the last decade was conducted using the terms “endoscopic/robotic thyroidectomy,” “patient satisfaction scores,” “questionnaire,” “quality of life,” and “cosmetic” in PubMed.

Results
Assessment methods for postoperative cosmetic satisfaction and sensory alterations encompassed verbal/visual analog scales, scar evaluations, Semmes–Weinstein monofilament tests, and more. The evaluation of postoperative quality of life in endoscopic thyroidectomy involved tools such as SF-36, SF-12, thyroid-specific questionnaires, thyroid cancer-specific quality of life questionnaires (THYCA-QOL), as well as assessments related to voice and swallow function. The cosmetic results of endoscopic thyroidectomy generally surpassed those of open thyroidectomy, while the quality of life in endoscopic procedures was either superior or equivalent to that in open thyroidectomy, especially with respect to general health, role emotion, and vitality.

Conclusions
Assessments of cosmetic outcomes and sensory alterations following endoscopic thyroidectomy predominantly relied on patients’ subjective feelings. The objective and subjective perspectives of scar assessments remain underutilized. In addition, postoperative laryngoscopy and voice function assessments in endoscopic thyroidectomy procedures require more attention.
Simulation of a control method for active kinesiotherapy with an upper extremity rehabilitation exoskeleton without force sensor
Authors: Falkowski Piotr , Jeznach Kajetan 
Published: February 2024

AbstractExoskeleton-aided active rehabilitation is a process that requires sensing and acting upon the motion intentions of the user. Typically, force sensors are used for this. However, they increase the weight and cost of these wearable devices. This paper presents the methodology for detecting users’ intentions only with encoders integrated with the drives. It is unique compared to other algorithms, as enables active kinesiotherapy while adding no sensory systems. The method is based on comparing the measured motion with the one computed with the idealised model of the multibody system. The investigation assesses the method’s performance and its robustness to model and measurement inaccuracies, as well as patients’ unintended motions. Moreover, the PID parameters are selected to provide the optimal regulation based on the dynamics requirements. The research proves the presented concept of the control approach. For all the tests with the final settings, the system reacts to a change in the user’s intention below one second and minimises the changes in proportion between the system’s acceleration and the generated user’s joint torque. The results are comparable to those obtained by EMG-based systems and significantly better than low-cost force sensors.
Exosome-derived lncRNA A1BG-AS1 attenuates the progression of prostate cancer depending on ZC3H13-mediated m6A modification
Authors: Yang Zhi , Luo Yu , Zhang Fan , Ma Likun 
Published: February 2024

Abstract
Background
Exosome-derived long non-coding RNAs (lncRNAs) and N6-methyladenosine (m6A) modifications of lncRNAs have been shown crucial functions in prostate cancer (PCa). Herein, we aim to investigate the detailed mechanism of exosome-derived lncRNA A1BG-AS1 in PCa process.

Methods
PCa cell exosomes were extracted, exosomal marker proteins (CD63, CD9) were detected utilizing western blotting, and exosomes with overexpressing A1BG-AS1 were co-cultured with targeted PCa cells. qRT-PCR was used to detect A1BG-AS1 expression and m6A methyltransferase ZC3H13 in PCa. Transwell, colony formation and CCK-8 assays were utilized to assess the invasion, migration, and proliferation ability of PCa cells. Then, we performed actinomycin D and MeRIP assays to analyze the regulatory effect of ZC3H13 on A1BG-AS1 mRNA stability and m6A modification level.

Results
We observed that A1BG-AS1 and ZC3H13 expression was restricted in PCa tumors. The invasion, proliferation and migratory capacities of PCa cells could be inhibited by up-regulating A1BG-AS1 or by co-culturing with exosomes that up-regulate A1BG-AS1. Additionally, ZC3H13 promoted stable A1BG-AS1 expression by regulating the m6A level of A1BG-AS1.

Conclusion
Exosomal A1BG-AS1 was m6A-modified by the m6A methyltransferase ZC3H13 to stabilize expression and thus prevent PCa cell malignancy. These findings offer a possible target for clinical therapy of PCa.
The effect of body mass index on short-term outcomes in patients undergoing off-pump coronary artery bypass grafting surgery: a retrospective study from a single cardiovascular center
Authors: Ding Chen-ying , Qi Wen-hui , An Yu-jie , Yuan Xin , Yao Yun-tai ,   the Evidence in Cardiovascular Anesthesia (EICA) Group
Published: February 2024

Abstract
Objective
This study is designed to investigate the impact of body mass index (BMI) on the short-term outcomes of patients undergoing off-pump coronary artery bypass graft (OPCAB) surgery.

Methods
Data was obtained from 1006 Chinese patients who underwent isolated, primary OPCAB at a high-traffic cardiovascular center during 2020. Subjects were categorized, by BMI, into a low & normal weight (LN) group (BMI < 24 kg/m2), an overweight (OVW) group (24 ≤ BMI < 28 kg/m2), and an obese (OBS) group (BMI ≥ 28 kg/m2). Information pertaining to patients’ short-term outcomes (including incidence of mortality and morbidities; duration of postoperative mechanical ventilation; length of stay in the ICU and hospital; postoperative bleeding; etc.) were extracted, and the data from each group were compared.

Results
The incidences of in-hospital mortality and morbidities were similar for all three groups. The volume of fluid infusion, postoperative bleeding within 24 h and total bleeding in LN group were higher than those in the OBS group (P < 0.001). The hemoglobin level was lower in the LN group than that in the OBS group (P < 0.001). Duration of mechanical ventilation and length of stay in the ICU in the LN group were longer than those in the OBS group (P < 0.001).

Conclusions
Our results demonstrate that BMI is not significantly related with short-term outcomes in OPCAB patients. However, we suggest that OPCAB patients with low-normal BMI are more susceptible to post-operative blood loss.
pyHiM: a new open-source, multi-platform software package for spatial genomics based on multiplexed DNA-FISH imaging
Authors: Devos Xavier , Fiche Jean-Bernard , Bardou Marion , Messina Olivier , Houbron Christophe , Gurgo Julian , Schaeffer Marie , Götz Markus , Walter Thomas , Mueller Florian , Nollmann Marcelo 
Published: February 2024

AbstractGenome-wide ensemble sequencing methods improved our understanding of chromatin organization in eukaryotes but lack the ability to capture single-cell heterogeneity and spatial organization. To overcome these limitations, new imaging-based methods have emerged, giving rise to the field of spatial genomics. Here, we present pyHiM, a user-friendly python toolbox specifically designed for the analysis of multiplexed DNA-FISH data and the reconstruction of chromatin traces in individual cells. pyHiM employs a modular architecture, allowing independent execution of analysis steps and customization according to sample specificity and computing resources. pyHiM aims to facilitate the democratization and standardization of spatial genomics analysis.
Field-based assessments of the seasonality of Culex pipiens sensu lato in England: an important enzootic vector of Usutu and West Nile viruses
Authors: Seechurn Nicola , Herdman M. Trent , Hernandez-Colina Arturo , Vaux Alexander G. C. , Johnston Colin , Berrell Morgan , Lopez Javier , Eckley Lindsay , Gonzalez-Olvera Merit , Gillespie Lisa , Kelly Paul Pearce , Baylis Matthew , Medlock Jolyon M. 
Published: February 2024

Abstract
Background
Usutu virus (USUV), which is closely related to West Nile virus (WNV), sharing a similar ecology and transmission cycle, was first reported in the UK in the southeast of England in 2020. Both USUV and WNV are emerging zoonotic viruses hosted by wild birds. The 2020 finding of USUV in England raised awareness of this virus and highlighted the importance of understanding the seasonality of Culexpipiens sensu lato (Cx. pipiens s.l.), the main enzootic vector of these viruses. Zoos are prime locations for trapping mosquitoes because of their infrastructure, security, and range of vertebrate hosts and aquatic habitats.

Methods
Three independent zoo-based case studies at four locations that cover the seasonality of Cx.pipiens s.l. in England were undertaken: (i) London Zoo (Zoological Society London [ZSL]) and surrounding areas, London; (ii) Chester Zoo (Cheshire); (ii) Twycross Zoo (Leicestershire); and (iv) Flamingo Land (zoo; North Yorkshire). Various adult mosquito traps were used to catch adult Cx.pipiens s.l. across seasons.

Results
High yields of Cx.pipiens s.l./Culex torrentium were observed in Biogents-Mosquitaire and Center for Disease Control and Prevention Gravid traps in all studies where these traps were used. Mosquito counts varied between sites and between years. Observations of adult Cx.pipiens s.l./Cx. torrentium abundance and modelling studies demonstrated peak adult abundance between late July and early August, with active adult female Cx.pipiens s.l./Cx. torrentium populations between May and September.

Conclusions
The information collated in this study illustrates the value of multiple mosquito monitoring approaches in zoos to describe the seasonality of this UK vector across multiple sites in England and provides a framework that can be used for ongoing and future surveillance programmes and disease risk management strategies.

Graphical Abstract
Hemicentral retinal vein occlusion in a patient with a history of coronavirus disease 2019 infection: a case report and review of the literature
Authors: Riazi-Esfahani Hamid , Sadeghi Reza , Soleymanzadeh Mahdi , Farrokhpour Hossein , Bazvand Fatemeh , Ebrahimiadib Nazanin , Khalili Pour Elias , Mirghorbani Masoud 
Published: February 2024

Abstract
Background
Considering the various manifestations of coronavirus disease 2019 and its imperative importance in terms of the right clinical approach and early management, we sought to present a hemicentral retinal vein occlusion case, with a history of heterozygosity of methylenetetrahydrofolate reductase (MTHFR) genes and potential for clotting complications as a late manifestation of coronavirus disease 2019, and provide a brief review of reported retinal vein occlusion cases in patients with coronavirus disease 2019.

Case presentation
A 35-year-old Iranian patient presented with a visual impairment in the left eye 4 months after recovering from coronavirus disease 2019. He reported a mild blurring of vision in the same eye a few days after admission due to coronavirus disease 2019. The ophthalmic evaluation was compatible with hemicentral retinal vein occlusion. Systemic and laboratory workups were negative except for borderline protein C activity, homocysteine levels, and heterozygosity of MTHFR genes. The patient was scheduled to receive three monthly intravitreal antivascular endothelial growth factor injections.

Conclusion
We present a case of inferior hemicentral retinal vein occlusion case with an MTHFR mutation with sequential loss of vision 4 months after coronavirus disease 2019 to make clinicians aware of the possibility of late ocular coronavirus disease 2019 manifestations.
Sex differences in the effects of individual anxiety state on regional responses to negative emotional scenes
Authors: Chaudhary Shefali , Wong Hak Kei , Chen Yu , Zhang Sheng , Li Chiang-Shan R. 
Published: February 2024

Abstract
Background
Men and women are known to show differences in the incidence and clinical manifestations of mood and anxiety disorders. Many imaging studies have investigated the neural correlates of sex differences in emotion processing. However, it remains unclear how anxiety might impact emotion processing differently in men and women.

Method
We recruited 119 healthy adults and assessed their levels of anxiety using State-Trait Anxiety Inventory (STAI) State score. With functional magnetic resonance imaging (fMRI), we examined regional responses to negative vs. neutral (Neg-Neu) picture matching in the Hariri task. Behavioral data were analyzed using regression and repeated-measures analysis of covariance with age as a covariate, and fMRI data were analyzed using a full-factorial model with sex as a factor and age as a covariate.

Results
Men and women did not differ in STAI score, or accuracy rate or reaction time (RT) (Neg-Neu). However, STAI scores correlated positively with RT (Neg-Neu) in women but not in men. Additionally, in women, STAI score correlated positively with lingual gyrus (LG) and negatively with medial prefrontal cortex (mPFC) and superior frontal gyrus (SFG) activity during Neg vs. Neu trials. The parameter estimates (βs) of mPFC also correlated with RT (Neg-Neu) in women but not in men. Generalized psychophysiological interaction (gPPI) analysis in women revealed mPFC connectivity with the right inferior frontal gyrus, right SFG, and left parahippocampal gyrus during Neg vs. Neu trials in positive correlation with both STAI score and RT (Neg-Neu). In a mediation analysis, mPFC gPPI but not mPFC activity fully mediated the association between STAI scores and RT (Neg-Neu).

Conclusion
With anxiety affecting the behavioral and neural responses to negative emotions in women but not in men and considering the known roles of the mPFC in emotion regulation, we discussed heightened sensitivity and regulatory demands during negative emotion processing as neurobehavioral markers of anxiety in women.
Dynamic routing optimization in software-defined networking based on a metaheuristic algorithm
Authors: Chen Junyan , Xiao Wei , Zhang Hongmei , Zuo Jiacheng , Li Xinmei 
Published: February 2024

AbstractOptimizing resource allocation and routing to satisfy service needs is paramount in large-scale networks. Software-defined networking (SDN) is a new network paradigm that decouples forwarding and control, enabling dynamic management and configuration through programming, which provides the possibility for deploying intelligent control algorithms (such as deep reinforcement learning algorithms) to solve network routing optimization problems in the network. Although these intelligent-based network routing optimization schemes can capture network state characteristics, they are prone to falling into local optima, resulting in poor convergence performance. In order to address this issue, this paper proposes an African Vulture Routing Optimization (AVRO) algorithm for achieving SDN routing optimization. AVRO is based on the African Vulture Optimization Algorithm (AVOA), a population-based metaheuristic intelligent optimization algorithm with global optimization ability and fast convergence speed advantages. First, we improve the population initialization method of the AVOA algorithm according to the characteristics of the network routing problem to enhance the algorithm’s perception capability towards network topology. Subsequently, we add an optimization phase to strengthen the development of the AVOA algorithm and achieve stable convergence effects. Finally, we model the network environment, define the network optimization objective, and perform comparative experiments with the baseline algorithms. The experimental results demonstrate that the routing algorithm has better network awareness, with a performance improvement of 16.9% compared to deep reinforcement learning algorithms and 71.8% compared to traditional routing schemes.
Reunion helper: an edge matcher for sibling fragment identification of the Dunhuang manuscript
Authors: Zheng Yutong , Li Xuelong , Weng Yu 
Published: February 2024

Abstract
The Dunhuang ancient manuscripts are an excellent and precious cultural heritage of humanity. However, due to their age, the vast majority of these treasures are damaged and fragmented. Faced with a wide range of sources and numerous fragments, the process of restoration generally involves two core elements: sibling fragments identification and fragment assembly. Currently, fragment restoration still heavily relies on manual labor. During the long practice, a consensus has been reached on the importance of edge features for not only assembly but also for identification. However, accurate extraction of edge features and their use for efficient identification requires extensive knowledge and strong memory. This is a challenge for the human brain. So that in previous studies, fragment edge features have been used for assembly validation but rarely for identification. Therefore, an edge matcher is proposed, working like a bloodhound, capable of “sniffing out” specific “flavors” in edge features and performing efficient sibling fragment identification accordingly, providing guidance when experts perform entity assembly subsequently. Firstly, the fragmented images are standardized. Secondly, traditional methods are used to compress the representation of fragment edges and obtain paired local edge images. Finally, these images are fed into the edge matcher for classification discrimination, which is a CNN-based pairwise similarity metric model proposed in this paper, introducing residual blocks and depthwise separable convolutions, and adding multi-scale convolutional layers. With the edge matcher, a complex matching problem is successfully transformed into a simple classification problem. In the absence of a standard public dataset, a Dunhuang manuscript fragment edge dataset is constructed. Experiments are conducted on that dataset, and the accuracy, precision, recall, and F1 scores of the edge matcher all exceeded 97%. The effectiveness of the edge matcher is demonstrated by comparative experiments, and the rationality of the method design is verified by ablation experiments. The method combines traditional methods and deep learning methods to creatively use the edge geometric features of fragments for sibling fragment identification in a natural rather than coded way, making full use of the computer’s computational and memory capabilities. The edge matcher can significantly reduce the time and scope of searching, matching, and inferring fragments, and assist in the reconstruction of Dunhuang ancient manuscript fragments.