bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisis Implementasi Program Poned Dalam Penurunan Angka Kematian Ibu di UPTD Puskesmas Patani
Authors: Akbar Suriyanti , Eka Vicky Yulivantina  , Yustina Ananti  
Published: February 2025

Angka Kematian Ibu di Maluku Utara mencapai 255 per 100. 000 kelahiran hidup pada tahun 2022, melebihi target SDG 70 per 100. 000. Penyebab utama kematian ibu adalah Perdarahan (21,14%), Hipertensi (26,34%), Infeksi (2,76%), Gangguan sistem peredaran darah (9,27%), dan faktor lainnya (40,49%). Kematian maternal terjadi 60,90% saat nifas, 26,33% saat hamil, dan 12,76% saat persalinan. Penelitian ini menggunakan pendekatan fenomenologi dengan informan Kepala Dinas Kesehatan, Kepala TU, Dokter, Perawat, Bidan, dan ibu nifas. Tujuan penelitian ini adalah menilai efektivitas kebijakan, mengeksplorasi tantangan sumber daya manusia dan komunikasi, serta mengidentifikasi karakteristik dan sikap pelaksana program. Hasil diharapkan dapat meningkatkan implementasi program untuk menurunkan Angka Kematian Ibu (AKI) di UPTD Puskesmas Patani berdasarkan teori Van Meter Van Horn. Instrumen penelitian menggunakan pedoman wawancara mendalam terkait pelaksanaan PONED. Berdasarkan 6 unsur dari teori tersebut, ditemukan bahwa: 1. Unsur standar dan tujuan kebijakan sudah berjalan. 2. Sumber daya manusia belum sesuai standar. 3. Komunikasi antar organisasi belum baik. 4. Karakteristik agen pelaksana sudah baik. 5. Sikap pelaksana sudah baik. 6. Lingkungan sosial, ekonomi, dan politik sudah baik. Implementasi program PONED di UPTD Puskesmas Patani sesuai teori Van Meter Van Horn untuk menurunkan Angka Kematian Ibu (AKI) sudah baik dalam 4 unsur: standar, ukuran, sasaran, dan tujuan kebijakan; karakteristik agen pelaksana; sikap pada pelaksana; dan lingkungan sosial, ekonomi, dan politik. Namun, 2 unsur yaitu sumber daya manusia dan komunikasi antar uni karakter organisasi belum berjalan baik. Penelitian ini sebaiknya fokus pada implementasi program PONED dan eksplorasi lebih mendalam tentang proses rujukan pasien dalam kegawatdaruratan.
The Hubungan Lama Pemakaian Suntik DMPA dengan Penurunan Libido pada Akseptor Suntik DMPA di Praktik Mandiri Bidan Hana Sri Wuryaningsih Parakan
Authors: Sri Wuryaningsih Hana , Rusliani Dian Monalisa , Hakim Riska Ismawati 
Published: February 2025

Long-term use of DMPA injectable contraceptives can lower estrogen levels, affect hormone metabolism, and lower serum estradiol levels, resulting in decreased female sexual function. Injectable DMPA birth control shows significant sexual dysfunction effects if used for more than 2 years. The purpose of this study was to determine the relationship between prolonged use and decreased libido in DMPA injectable birth control acceptors at PMB Hana Sri Wuryaningsih. The research design uses descriptive correlative with a cross sectional approach. The population in this study was all DMPA injectable KB acceptors at PMB Hana Sri Wuryaningsih as many as 141 people. The sampling technique uses Purposive sampling. The number of samples was 104 respondents. This research instrument used questionnaires. Univariate analysis with frequency distribution table and bivariate analysis with Chi Square test.
„Martwa” natura – nieposłuszne (nie)ekfrazy w wierszach juwenilnych Juliana Kornhausera
Authors: Piotrowska-Grot Magdalena 
Published: February 2025

Celem artykułu jest analiza oraz próba zaklasyfikowania dwóch juwenilnych tekstów Juliana Kornhausera – Martwa natura z kompotierką (Bernard Buffet) oraz Guernica (Pablo Picasso) – które nawiązują do dzieł malarskich. Właśnie obrazom w twórczości autora Origami warto poświęcić chwilę uwagi, malarstwo pozostaje bowiem niezwykle ważnym źródłem odniesień tekstów Kornhausera. Wybrane do analizy przykłady nie mieszczą się jednak w definicyjnych ramach ekfrazy czy hypotypozy. Co ważne, prowadzą do odkrycia poetyckich powiązań Kornhausera z surrealizmem, głównie w wydaniu Bretonowskim, pozwalają więc na odnalezienie drogowskazów interpretacyjnych, które okazują się niezwykle pomocne w trakcie lektury kolejnych, zwłaszcza publikowanych w latach siedemdziesiątych, wierszy i powieści autora Zjadaczy kartofli. Interpretacja wierszy juwenilnych uprawnia więc do poszukiwania w tej twórczości eksperymentów, które nie tylko wymykają się metodologicznym dookreśleniom, lecz także stanowią dowód na korelacje poezji Kornhausera z poetykami awangardowymi.
Analisis Status Mutu Air pada Saluran Perairan Pembuangan Limbah Cair Batik dengan Metode Indeks Pencemaran di Desa Kebon, Kecamatan Bayat, Kabupaten Klaten
Authors: Wicaksono Aditya Pandu , Wardani Resita 
Published: February 2025

Lokasi penelitian berada di Desa Kebon, Kecamatan Bayat, Kabupaten Klaten. Daerah penelitian merupakan daerah yang terkenal dengan industri batik rumah tangga. Limbah cair batik dari industri batik rumah tangga di lokasi penelitian rata-rata belum diolah terlebih dahulu sebelum dibuang ke saluran air. Tujuan penelitian ini adalah menganalisis status mutu air pada saluran perairan pembuangan limbah cair batik di lokasi penelitian. Metode yang digunakan dalam penelitian ini berupa metode pengambilan sampel dengan metode purposive sampling; metode analisis laboratorium; serta metode evaluasi dan analisis data dengan metode indeks pencemaran. Parameter yang diuji pada penelitian ini yaitu Fenol, Krom Total, COD dan BOD. Hasil analisis laboratorium menunjukkan bahwa pada input (limbah cair batik) didapatkan nilai sebesar Fenol 1,3813 mg/L; Krom Total 0,2262 mg/L; COD 9.138,996 mg/L; dan BOD 5.693,5945 mg/L. Sedangkan pada bagian output (saluran air) sebesar Fenol 0,2571 mg/L; Krom Total 0,3384 mg/L; COD 168,726 mg/L; dan BOD 94,1491 mg/L. Tingkat pencemaran limbah cair batik pada parameter yang melebihi baku mutu yaitu Fenol, COD dan BOD. Status mutu air di lokasi penelitian tergolong tercemar ringan hingga tercemar sedang.
Kajian Kandungan Logam Berat Unsur Ni, Cu Dan Zn Pada Mataair Panas Parangwedang, Di Desa Parangtritis, Kapanewon Kretek, Kabupaten Bantul, Daerah Istimewa Yogyakarta
Authors: Mutmainah Mutmainah , Irawan Agus Bambang , Lukito Herwin , Algary Tissia Ayu , Wicaksono Aditya Pandu 
Published: February 2025

Daerah penelitian terletak di Desa Parangtritis, Kapanewon Kretek, Kbaupaten Bantul, DIY. Daerah penelitian merupakan daerah dengan manifestasi panas bumi berupa mataair panas, Mataair panas tersebut dikenal dengan nama matair panas Parangwedang. Tujuan penelitian ini adalah mengetahui tipe mataair panas dan mengetahui kualitas mataair panas berdasarkan logam berat yang terlarut pada mataair panas di lokasi penelitian. Metode yang digunakan untuk mengetahui tipe mataair panas yaitu dengan menggunakan diagram segitiga Cl-SO4-HCO3 dan kualitas mataair panas berdasarkan pada parameter logam berat Ni, Cu dan Zn. Hasil penelitian menunjukkan tipe mataair panas termasuk tipe Mature Water, yaitu tipe dengan kandungan klorida yang berlimpah. Kualitas mataair panas berdasarkan parameter Ni, Cu dan Zn termasuk kedalam kategori tidak baik karena melebihi baku mutu. Konsentrasi Ni yang didapatkan sebesar 88.2 ppm, konsentrasi Cu sebesar 29.8 ppm dan konsentrasi Zn sebesar 13.56 ppm. Sedangkan untuk baku mutu masing-masing paramater yaitu Ni (0.05 ppm), Cu (0.02 ppm) dan Zn (0.05 ppm).
Using Context Clues Strategy on Reading Comprehension
Authors: Eka Eka Oktaviyanti , Siti Rohbiah Tatu , Rahmawati Eulis 
Published: February 2025

This study measured the effectiveness of reading comprehension in English. Therefore, the purpose of this study is to find out whether there is an effect in using the new strategy so as to train learners to know foreign vocabulary by using context clues. The method used was quantitative quasi-experiment consisting of 2 classes, the population was taken from seventh grade students. namely class VII A as the control group and class VII C as the experimental group. each class amounted to 30 learners. Data were obtained by performance tests containing descriptive text. Based on the test results, the calculated T value is 12.986 ˃ T table 1.701 so it shows Ho is rejected and Ha is accepted. This is significant based on the percent of reading comprehension of seventh grade students at MTs Negeri 3 Cilegon City by 85.8% and the remaining 14.2% is influenced by other variables. This shows that the data that has been obtained there is an increase in students' reading comprehension in descriptive text after using the Context Clues Strategy.
Novel, low-cost bioreactor for in vitro electrical stimulation of cardiac cells
Authors: Licata Joseph P. , Gerstenhaber Jonathan A. , Lelkes Peter I. 
Published: February 2025

IntroductionThe successful implantation of laboratory-grown cardiac tissue requires phenotypically mature cardiomyocytes capable of electrophysiological integration with native heart tissue. Pulsed electrical stimulation (ES) has been identified as a promising strategy for enhancing cardiomyocyte maturation. However, there are discrepancies in the literature as to best practices for promoting cardiac differentiation using ES.MethodsThis study presents a novel, 3D printed bioreactor that delivers in vitro ES to human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), promoting cell maturity and functional readiness for implantation. Finite element analysis and mathematical modeling were used to model the fluid dynamics and to characterize in detail the delivery of pulsatile electrical signals, providing precise control over stimulation parameters such as voltage, current, and charge.ResultsThe bioreactor developed here provides an easy-to-use, inexpensive platform for culturing hiPSC-CMs under the influence of ES and low-shear fluid flow for enhanced nutrient availability, while its “drop-in” design facilitates real-time observation of cultured cells. The electrical stimulation provided is controlled, modeled, and predictable, enabling reproducible experimental conditions and promoting comparability across future studies. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) grown in the bioreactor with ES showed improved differentiation and an enhanced ability to respond to external electrical pacing signals.DiscussionBy offering a standardized platform for ES-based cardiomyocyte maturation, this bioreactor aims to accelerate advancements in cardiac tissue engineering. Future research will explore how variations in ES parameters influence cardiomyocyte phenotype and maturation, contributing to a deeper understanding of cardiac cell development and optimization for therapeutic applications.
Understanding pre-service teachers' intention to adopt and use artificial intelligence in Nigerian inclusive classrooms
Authors: Adigun Olufemi Timothy , Tijani Faisat Adeniran , Haihambo Cynthy K. , Enock Simasiku Limbo 
Published: February 2025

IntroductionThis study applied the Unified Theory of Acceptance and Use of Technology (UTAUT) to provide an understanding of the behavioral intentions of pre-service teachers in the adoption and utilization of artificial intelligence (AI) tools for educational engagement in the inclusive classroom.MethodsThe cross-sectional study collected data through a validated questionnaire from 411 pre-service teachers were analyzed with descriptive statistics such as frequency counts and simple percentage calculation, as well as inferential statistics which involved correlational analysis and Structural Equation Modeling (SEM).ResultsThe study established that effort expectancy had a positive and direct significant contribution to the perceived behavioral intention of pre-service teachers to adopt and use AI for inclusive education teaching. Technological self-efficacy had no direct contributory effect on these teachers' behavioral intention to adopt and use AI for inclusive education teaching. Technological self-efficacy did, however, have a significant positive and indirect contribution to the effect of performance expectancy and social influence on the pre-service teachers' behavioral intention to adopt and use AI for inclusive education teaching, based on their technological self-efficacy.DiscussionThe implication of findings of this study points to the exigency of a need to strengthen institutional policies and teacher preparation curricula in a manner that would advance the infusion of the use of artificial intelligence for teaching of learners with special needs.
Association between Helicobacter pylori infection, serum thyroid-stimulating hormone, and thyroxine in the National Health and Nutrition Examination Survey 1999–2000
Authors: Lu Ting , Lu Shunshun , Lin Jieqiong , Shao Xiaona , Chen Dahua , Shen Jianwei 
Published: February 2025

BackgroundHelicobacter pylori has been increasingly implicated in extra-gastric diseases. Current evidence regarding the association between serum thyroid-stimulating hormone (TSH), thyroxine (T4), and H. pylori infection remains inconclusive. Consequently, this study aimed to explore the correlation between TSH and T4 levels and H. pylori infection in a US-based population sample.MethodsData from the US National Health and Nutrition Examination Survey (NHANES), comprising 971 participants aged 30–85 years from 1999 to 2000, were analyzed. Binary logistic regression was employed to analyze the correlation between H. pylori and TSH and T4 levels. The impact of TSH and T4 on H. pylori infection was further assessed using restricted cubic spline (RCS) analysis. In addition, subgroup analyses stratified by sex and age were conducted.ResultsSubjects with H. pylori seropositivity demonstrated lower serum TSH levels and higher serum T4 levels compared to those with H. pylori seronegativity. A significant positive correlation was identified between H. pylori seropositivity and T4 levels with increasing quartiles of hormonal levels in both univariate regression models (Q4 vs. Q1: OR = 1.483; 95% CI, 1.033–2.129) and multivariate regression models (Q4 vs. Q1: OR = 1.004; 95% CI, 0.981–1.026). Conversely, a negative correlation was observed between H. pylori seropositivity and TSH levels with increasing quartiles of hormonal levels in univariate regression models (Q4 vs. Q1: OR = 0.579; 95% CI, 0.403–0.831) and in multivariate regression models (Q4 vs. Q1: OR = 0.580; 95% CI, 0.389–0.866). In stratified analyses, the adjusted association of serum T4 levels with H. pylori seropositivity was statistically significant among men (T4: Q4 vs. Q1: OR = 2.253; 95% CI, 1.311–3.873), age over 68 years in TSH levels (Q4 vs. Q1: OR = 0.434; 95% CI, 0.206–0.911), and age 41–54 years in T4 levels (Q4 vs. Q1: OR = 4.965; 95% CI, 2.071–11.903). RCS analysis revealed a non-linear relationship between TSH levels and H. pylori infection. Notably, when TSH &lt; 0.98 IU/ml, the likelihood of H. pylori infection significantly increased.ConclusionsLower TSH and higher T4 levels were associated with H. pylori infection, particularly among men and elderly individuals.
Development of research on HIV-associated neurocognitive disorder and emerging trends: a visualization analysis via CiteSpace
Authors: Zhou Tingting , Chen Xuannan , Lai Yu 
Published: February 2025

BackgroundIn the combination antiretroviral therapy era, HIV-associated neurocognitive disorder (HAND) is still widespread among HIV-infected individuals. However, there is no effective treatment for HAND, and the exact pathogenic mechanism of HAND remains unknown. This paper aims to provide a reference for further exploration in the field of HAND research.MethodsWe used CiteSpace software to collect 3057 articles related to HAND in the Web of Science Core Collection for comprehensive analysis. Betweenness centrality, count, and burst values were used as indicators in the visualization analysis, aiming to predict future new directions and cutting-edge trends.ResultsThe last decade has been the peak period of HAND research, with the most prominent contributions by authors, countries, and institutions being Grant, Igor (135), the USA (2211), and the University of California System (758), respectively. The most frequently cited article is “HIV-associated neurocognitive disorders persist in the area of potent antiretroviral therapy: CHARTER Study.” The hotspots in this field are “neurocognitive impairment,” “central nervous system,” “cerebrospinal fluid,” “HIV-1 tat,” “SIV,” “inflammation,” “infection,” and “pathogenesis.” The current research direction of HAND is focused on exploring the pathogenic mechanism underlying HIV-associated neurocognitive impairment and potential therapeutic targets.ConclusionThis study provides a bibliometric visualization of HAND-related literature to gain insight into the development and frontiers of this research field. The study also provides scholars with detailed references and identifies future research directions to better promote the development of this field of research.
Exploring the exoproteome of the parasitic nematode Anisakis simplex (s. s.) and its impact on the human host – an in vitro cross-talk proteomic approach
Authors: Stryiński Robert , Fiedorowicz Ewa , Mateos Jesús , Andronowska Aneta , Łopieńska-Biernat Elżbieta , Carrera Mónica 
Published: February 2025

IntroductionAnisakis simplex sensu stricto (s. s.) is one of the most widespread parasitic nematodes of marine organisms, with humans as accidental hosts. While many studies have explored nematode biology and host interactions, the role of extracellular vesicles (EVs) as signaling molecules in parasitic nematodes is less understood.Materials and methodsTherefore, the proteins present in the EVs of A. simplex (s. s.) (Anis-EVs) were identified. In addition, a cross-talk proteomic approach was used to identify differentially regulated proteins (DRPs) in the proteome of the human intestinal epithelial cell line (Caco-2) co-cultured with L3 larvae of A. simplex (s. s.) or directly exposed to two concentrations (low or high) of Anis-EVs. In addition, DRPs were identified in the proteome of A. simplex (s. s.) larvae affected by co-culture with Caco-2. To achieve this goal, the shotgun proteomics method based on isobaric mass labeling (via tandem mass tags; TMT) was used with a combination of nano high-performance liquid chromatography (nLC) coupled with an LTQ-Orbitrap Elite mass spectrometer. In addition, ELISA assays were used to demonstrate if Caco-2 respond to A. simplex (s. s.) larvae and Anis-EVs with significant changes in selected cytokines secretion.ResultsThe results of this study indicate the anti-inflammatory character of Anis-EVs in relation to Caco-2. At the same time, direct treatment with Anis-EVs resulted in more significant changes in the Caco-2 proteome than co-culture with L3 larvae.DiscussionThe results obtained should lead to a better understanding of the molecular mechanisms underlying the development of A. simplex (s. s.) infection in humans and will complement the existing knowledge on the role of EVs in host-parasite communication.
Identification of novel autoantibodies in Sjögren’s disease
Authors: Engelke Fiona , Budde Petra , De Vita Salvatore , Dörner Thomas , Ernst Diana , Gras Jan , Heidecke Harald , Kilian Annika Loredana , Kniesch Katja , Lindemann Ann-Sophie , Quartuccio Luca , Ritter Jacob , Schulze-Forster Kai , Seeliger Benjamin , Zucht Hans-Dieter , Witte Torsten 
Published: February 2025

IntroductionThe diagnosis of Sjögren’s disease (SjD) in patients without autoantibodies against Ro/SSA is a major challenge. We aimed to identify novel autoantibodies in SjD that may facilitate the diagnostic procedure for Ro/SSA negative SjD.MethodsIgG and IgA autoantibody reactivity of 94 potential candidate autoantigens for SjD, selected from a discovery screen of 1,629 human antigens coupled to Luminex beads and prior knowledge about potential biological relevance, were examined in serum of SjD patients (n=347) using Luminex and ELISA technology. Healthy (HC, n=118) and non-Sjögren’s sicca syndrome (NSS, n=44) individuals served as controls. To assess disease specificity, the novel autoantibodies were also measured in serum of patients with Rheumatoid Arthritis (RA, n=50), Systemic Lupus Erythematosus (SLE, n=49), and Systemic Sclerosis (SSc, n=37). Results45 novel autoantibodies were significantly (p ≤ 0.05) more prevalent in SjD than in HC and were detected in up to 19% of the SjD cohort. The most common autoantibodies were against CCL4, M5, TMPO and OAS3. Some of the novel autoantibodies were associated with extraglandular disease manifestations, such as anti-TONSL or anti-IL6 with pulmonary involvement. We have developed a three and five marker panel for the detection of Ro/SSA negative patients, consisting of anti-FNBP4, anti-SNRPC, anti-CCL4, anti-M3 and anti-KDM6B, which had a sensitivity of up to 46% with a specificity of 95% (SjD vs. HC). Both panels discriminate these patients from HC, whereas the three-marker more effectively differentiates between Ro/SSA negative patients and NSS.DiscussionNovel autoantibodies will facilitate the diagnosis of Ro/SSA negative patients with SjD, in particular our predictive panel will be useful in the diagnosis and differentiation of these patients from healthy and NSS individuals in a clinical context. In addition, the autoantibodies may also be useful for risk stratification of extraglandular manifestations.
Secreted PD-L1 alleviates inflammatory arthritis in mice through local and systemic AAV gene therapy
Authors: Li Wenjun , Sun Junjiang , Feng Susi , La Rosa Ariana , Zhang Panli , Wu Eveline Y. , Loeser Richard , Li Chengwen 
Published: February 2025

IntroductionRheumatoid arthritis (RA) primarily affects the joints but can also affect multiple organs and profoundly impacts patients’ ability to carry out daily activities, mental health, and life expectancy. Current treatments for RA are limited in terms of duration, efficacy, and adverse effects. PD-L1 is a checkpoint protein that plays important roles in immune regulation and has been implicated in the initiation and progression of multiple autoimmune diseases.MethodIn a previous study, we demonstrated that intra-articular injection with adeno-associated virus (AAV) vectors encoding wild type PD-L1 improved local inflammation in the joint in the collagen-induced arthritis (CIA) mouse model of RA. To further improve efficacy, we explored AAV-mediated delivery of the soluble PD-L1 (sPD-L1) to CIA mice.ResultAfter intra-articular injection of AAV6 vectors expressing the optimal isoform of sPD-L1 (shPD-L1), more potency was observed when compared to wild type PD-L1, with a lower dose of AAV6/shPD-L1 needed for arthritis improvement. To study the therapeutic effect of systemic expression of sPD-L1, we administered AAV8/shPD-L1 gene therapy in CIA mice via retro-orbital injection and found significant improvements in joint inflammation and paw swelling, exhibiting similar phenotypes to that in naïve mice. The levels of total immunoglobulin and anti-collagen specific antibodies were lower in AAV8/shPD-L1 treated CIA mice than those in controls. The levels of pro-inflammatory cytokines in blood were also significantly decreased in shPD-L1 treated mice. Additionally, T cell apoptosis rates in the spleen showed a 2-fold increase in treated mice. Finally, we investigated the therapeutic effect of AAV/shPD-L1 via intramuscular injection. After injection of AAV6/shPD-L1, decreased paw swelling, reduced joint inflammation, and lower levels of pro-inflammatory cytokines in blood were achieved. The therapeutic effect of shPD-L1 was dose dependent via intramuscular treatment with AAV vectors.ConclusionIn conclusion, the findings in this study suggest that intra-articular injection of AAV vectors encoding sPD-L1 results in greater therapeutic benefit on arthritis, and systemic AAV/sPD-L1 is able to block the development of inflammatory arthritis with inhibition of the systemic immune response, underlining the potential of gene therapy with systemic delivery of shPD-L1 via AAV vectors in RA.