bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacteroidaceae, Bacteroides, and Veillonella: emerging protectors against Graves’ disease
Authors: Liu Siyuan , Li Fan , Cai Yunjia , Ren Linan , Sun Lin , Gang Xiaokun , Wang Guixia 
Published: February 2024

BackgroundGraves’ disease (GD) is the most common cause of hyperthyroidism, and its pathogenesis remains incompletely elucidated. Numerous studies have implicated the gut microbiota in the development of thyroid disorders. This study employs Mendelian randomization analysis to investigate the characteristics of gut microbiota in GD patients, aiming to offer novel insights into the etiology and treatment of Graves’ disease.MethodsTwo-sample Mendelian randomization (MR) analysis was employed to assess the causal relationship between Graves’ disease and the gut microbiota composition. Gut microbiota data were sourced from the international consortium MiBioGen, while Graves’ disease data were obtained from FINNGEN. Eligible single nucleotide polymorphisms (SNPs) were selected as instrumental variables. Multiple analysis methods, including inverse variance-weighted (IVW), MR-Egger regression, weighted median, weighted mode, and MR-RAPS, were utilized. Sensitivity analyses were conducted employing MR-Egger intercept test, Cochran’s Q test, and leave-one-out analysis as quality control measures.ResultsThe Mendelian randomization study conducted in a European population revealed a decreased risk of Graves’ disease associated with Bacteroidaceae (Odds ratio (OR) [95% confidence interval (CI)]: 0.89 [0.89 ~ 0.90], adjusted P value: &lt;0.001), Bacteroides (OR: [95% CI]: 0.555 [0.437 ~ 0.706], adjusted P value: &lt;0.001), and Veillonella (OR [95% CI]: 0.632 [0.492 ~ 0.811], adjusted P value: 0.016). No significant evidence of heterogeneity, or horizontal pleiotropy was detected. Furthermore, the preliminary MR analysis identified 13 bacterial species including Eubacterium brachy group and Family XIII AD3011 group, exhibiting significant associations with Graves’ disease onset, suggesting potential causal effects.ConclusionA causal relationship exists between gut microbiota and Graves’ disease. Bacteroidaceae, Bacteroides, and Veillonella emerge as protective factors against Graves’ disease development. Prospective probiotic supplementation may offer a novel avenue for adjunctive treatment in the management of Graves’ disease in the future.
Transcriptome analysis reveals ABA involved in the detoxification mechanism of macroalga Gracilariopsis lemaneiformis to cadmium toxicity
Authors: Chen Xiaojiao , Tang Yueyao , Sun Xue , Zhang Hao , Xu Nianjun 
Published: February 2024

IntroductionCadmium (Cd) is a significant threat environmental pollutant in the marine ecological environment offshore. The macroalga, Gracilariopsis lemaneiformis, of significant economic value, is widely cultivated along China’s coastline. Yet, little is known about the molecular mechanisms underlying Cd tolerance in macroalga.MethodsHere, we examined the transcriptome of G. lemaneiformis exposed to Cd to identify the responses to Cd stress.Results and discussionOur findings revealed that Cd led to the retardation of growth rate in G. lemaneiformis, accompanied by a notable reduction in the content of photosynthetic pigments and a decrease in the expression of genes associated with the photosynthetic system and nitrogen metabolism. When exposed to Cd, there was a rapid increase in Cd levels through the upregulation of genes encoding GlZIP6 and GlIRT1. Additionally, the expression of Cd efflux transporters, GlZIP1 and GlABCG22, and the ABCC7 transporter for compartmentation to the vacuole, was induced to mitigate Cd toxicity. Cd also activated crucial genes involved in the ABA biosynthesis and enhanced ABA content, thereby inducing ABA signaling pathway. Furthermore, exogenous ABA reduced the growth inhibition of G. lemaneiformis under Cd stress. Redox homeostasis was adjusted to adapt to Cd toxicity, with thioredoxin, glutaredoxin cycle and ascorbate-glutathione cycle identified as playing significant in maintaining reactive oxygen species homeostasis. Moreover, transcription factors such as several MYBs, signal transmission factors G protein and heat shock proteins (sHSPs, HSP 40, HSP 90, HSP101) were involved in the detoxification of Cd. Collectively, this study provided a comprehensive understanding of the molecular mechanisms underpinning the of responses of macroalga G. lemaneiformis to Cd exposure.
An immune-related gene prognostic prediction risk model for neoadjuvant chemoradiotherapy in rectal cancer using artificial intelligence
Authors: Shu Pei , Liu Ning , Luo Xu , Tang Yuanling , Chen Zhebin , Li Dandan , Miao Dong , Duan Jiayu , Yan Ouying , Sheng Leiming , Ouyang Ganlu , Wang Sen , Jiang Dan , Deng Xiangbing , Wang Ziqiang , Li Qingyun , Wang Xin 
Published: February 2024

BackgroundThis study aimed to establish and validate a prognostic model based on immune-related genes (IRGPM) for predicting disease-free survival (DFS) in patients with locally advanced rectal cancer (LARC) undergoing neoadjuvant chemoradiotherapy, and to elucidate the immune profiles associated with different prognostic outcomes.MethodsTranscriptomic and clinical data were sourced from the Gene Expression Omnibus (GEO) database and the West China Hospital database. We focused on genes from the RNA immune-oncology panel. The elastic net approach was employed to pinpoint immune-related genes significantly impacting DFS. We developed the IRGPM for rectal cancer using the random forest technique. Based on the IRGPM, we calculated prognostic risk scores to categorize patients into high-risk and low-risk groups. Comparative analysis of immune characteristics between these groups was conducted.ResultsIn this study, 407 LARC samples were analyzed. The elastic net identified a signature of 20 immune-related genes, forming the basis of the IRGPM. Kaplan−Meier survival analysis revealed a lower 5-year DFS in the high-risk group compared to the low-risk group. The receiver operating characteristic (ROC) curve affirmed the model’s robust predictive capability. Validation of the model was performed in the GSE190826 cohort and our institution’s cohort. Gene expression differences between high-risk and low-risk groups predominantly related to cytokine−cytokine receptor interactions. Notably, the low-risk group exhibited higher immune scores. Further analysis indicated a greater presence of activated B cells, activated CD8 T cells, central memory CD8 T cells, macrophages, T follicular helper cells, and type 2 helper cells in the low-risk group. Additionally, immune checkpoint analysis revealed elevated PDCD1 expression in the low-risk group.ConclusionsThe IRGPM, developed through random forest and elastic net methodologies, demonstrates potential in distinguishing DFS among LARC patients receiving standard treatment. Notably, the low-risk group, as defined by the IRGPM, showed enhanced activation of adaptive immune responses within the tumor microenvironment.
Case report: Exploring the utility of whole-body bone scintigraphy for pediatric Langerhans cell histiocytosis: insights from clinical practice
Authors: Song Wenyu , Hu Fan , Shi Wei , Wang Fang , Zhang Yongxue , Lan Xiaoli , Xia Xiaotian 
Published: February 2024

PurposeThis mini-review delves into the realm of Langerhans cell histiocytosis (LCH) in children, focusing on its skeletal involvement. By synthesizing pertinent literature, we sought to provide a comprehensive understanding of LCH’s clinical and radiographic spectrum. Our study then demonstrates the diagnostic prowess of whole-body 99mTc-methyl diphosphonate (MDP) scintigraphy in LCH cases, underscoring its value in tandem with existing knowledge.MethodsOur approach involved an extensive literature review that contextualized LCH within the current medical landscape. Subsequently, we presented a case series featuring five pediatric instances of skeletal LCH, one accompanied by soft tissue infiltration. The principal aim was to illuminate the diagnostic and staging potential of whole-body 99mTc-MDP scintigraphy, augmenting existing insights.ResultsThrough meticulous literature synthesis, we highlighted pediatric LCH’s protean clinical manifestations and radiological variability. Aligning with this spectrum, our case series underscored the role of 99mTc-MDP scintigraphy in diagnosing and staging LCH. Among the five pediatric cases, one demonstrated concurrent soft tissue involvement. This aligns with the multifaceted nature of LCH presentations.ConclusionPediatric LCH can present with a wide range of clinical and radiologic features. By amalgamating our cases with extant literature, we stress the necessity of a multimodal strategy. 99mTc-MDP scintigraphy emerged as an indispensable tool for accurate staging and soft tissue detection. Our findings collectively advocate for a holistic approach to managing LCH, ensuring informed therapeutic decisions for optimal patient outcomes.
BK virus-associated hemorrhagic cystitis in pediatric stem cell transplantation: a case report and scoping review
Authors: Moss Julia E. , Muller William J. 
Published: February 2024

IntroductionBK virus-associated hemorrhagic cystitis (BK-HC) is a debilitating and poorly understood complication of hematopoietic stem cell transplantation (SCT). Hematuria, dysuria, and other symptoms associated with BK-HC are common in the immediate post-SCT period, making BK-HC difficult to distinguish from other conditions presenting with these symptoms. Despite published criteria for diagnosis, the degree to which these criteria are consistently applied to either clinical diagnosis or to studies informing BK-HC management is unclear. We present a case of BK-HC in a pediatric SCT recipient, and discuss the challenges associated with treatment in the absence of rigorous data to inform clinical management.MethodsWe reviewed all cases of BK viruria at our center in patients undergoing SCT between January 2015 and December 2019. We then performed a scoping review of publications in PubMed addressing BK-HC, specifically focusing on how BK-HC was defined. Publications using the keywords “BK polyomavirus” and “hemorrhagic cystitis” were included if they involved a clinical study of SCT recipients and a full-text article was available in English. Case reports were excluded. Analysis focused on whether BK-HC was explicitly defined and whether the definition incorporated elements of diagnostic criteria published by European Conference on Infections in Leukemia (ECIL).ResultsA total of 30 studies published between January 2018 and 30 June 2021 met criteria for review, including 4 clinical trials, 7 prospective observational studies, and 19 retrospective observational studies. Fifteen of these studies included pediatric patients (7 pediatric only, 8 combined adult and pediatric). Of the 30 publications, 19 included a definition of either BK-HC or BK cystitis, with only five using ECIL criteria, all of which were observational studies. Multiple interventions are described for treatment of BK-HC, including cidofovir, leflunomide, quinolones, hyperbaric oxygen, keratinocyte growth factor, and BK-specific cytotoxic T lymphocytes. However, evidence to support efficacy for any of these interventions is lacking.DiscussionAlthough BK-HC is a well-known complication of SCT, evidence to support available treatment options is limited. Well-controlled studies that incorporate clear diagnostic criteria are needed to better define the risk factors, natural history, and ideal interventions.
Effect of physical exercise on the emotional and cognitive levels of patients with substance use disorder: a meta-analysis
Authors: Zheng Yamiao , Zhao Yiyang , Chen Xilian , Li Shanshan 
Published: February 2024

IntroductionThis study investigated the impact of different modes of physical exercise on the emotional and cognitive levels of patients with Substance Use Disorder (SUD). By exploring the most effective intervention types, cycle, frequency, and duration, we aimed to provide evidence-based recommendations for the adjunctive treatment of SUD.MethodsWe conducted a systematic search in five databases, including PubMed, Web of Science, The Cochrane Library, ScienceDirect, and EBSCO, from database inception up to May 2023, and identified 4,255 randomized controlled trials addressing the influence of physical exercise on the emotional and cognitive levels of SUD patients. Data extraction and analysis were performed using Review Manager 5.4 software, focusing on 11 studies that met the inclusion criteria and included 895 participants. Subsequently, a meta-analysis was conducted using Stata 16.0 software, presenting the results in the form of standardized mean differences (SMD) and 95% confidence intervals (CI).ResultsOur findings indicate that physical exercise significantly alleviates anxiety and depression in SUD patients while improving their cognitive function. Specifically, physical exercise was found to reduce anxiety (SMD = −0.726 [−1.349, −0.103], p &lt; 0.05) and depression (SMD = −0.666 [−1.077, −0.255], p &lt; 0.05) and enhance cognitive levels (SMD = −0.523 [−0.887, −0.159], p &lt; 0.05) among patients. Subgroup analysis further revealed that SUD patients benefitted most from physical exercise when engaging in aerobic exercises lasting over 12 weeks, with a frequency exceeding 40 sessions and each session lasting more than 60 min.DiscussionIn conclusion, our study affirms that physical exercise mitigates anxiety and depression while enhancing cognitive function in SUD patients, making it an effective measure for adjunctive clinical treatment.
Feasibility of Recovery Assessment Scale – Domains and Stages (RAS-DS) for everyday mental health practice
Authors: Ramesh Shivani , Scanlan Justin Newton , Honey Anne , Hancock Nicola 
Published: February 2024

IntroductionRoutine use of self-rated measures of mental health recovery can support recovery-oriented practice. However, to be widely adopted, outcome measures must be feasible. This study examined the feasibility of Recovery Assessment Scale – Domains and Stages (RAS-DS) from the perspectives of mental health workers.MethodMental health workers who had previously sought permission to use RAS-DS (n=58) completed an online survey that explored three aspects of feasibility: practicality, acceptability and applicability.ResultsThe highest-rated feasibility items related to applicability, or usefulness in practice, with over 90% of participants reporting that RAS-DS helps “promote discussion” and covers areas that are “meaningful to consumers”. Acceptability items indicated that the purpose of RAS-DS is clear but length was an issue for some participants. At a practical level, RAS-DS was seen as easy to access but training was seen by many as necessary to ensure optimal use.ConclusionResults suggest potential usefulness of RAS-DS as a routine outcome measure and identify aspects that can be addressed to further enhance feasibility including provision of training materials and opportunities, wide-reaching promotion of its use as a collaborative tool, and further investigation of issues around instrument length.
Spatial, geographic, and demographic factors associated with adolescent and youth suicide: a systematic review study
Authors: Ghadipasha Masoud , Talaie Ramin , Mahmoodi Zohreh , Karimi Salah Eddin , Forouzesh Mehdi , Morsalpour Masoud , Mahdavi Seyed Amirhosein , Mousavi Seyed Shahram , Ashrafiesfahani Shayesteh , Kordrostami Roya , Dadashzadehasl Nahid 
Published: February 2024

BackgroundSuicide is a public health issue and a main cause of mortality among adolescents and the youth worldwide, particularly in developing countries.ObjectivesThe present research is a systematic review aiming to investigate the spatial, geographical, and demographic factors related to suicide among adolescents and the youth.MethodsIn this systematic review, two researchers examined PsycINFO, Web of Science, Scopus, and PubMed databases on December 7th, 2022 with no time limits from the beginning of publication until 2022 to identify the primary studies on spatial and geographic analysis on adolescent and youth suicides. Once duplicate studies were identified and removed, the titles and abstracts of studies were examined and irrelevant studies were also removed. Finally, 22 studies were reviewed based on the inclusion criteria.ResultsOur findings show that suicide rates are generally higher among men, residents of rural and less densely populated regions, coastal and mountainous regions, natives, 15-29 age group, less privileged populations with social fragmentation, unemployed, divorced or lonely people, those who live in single parent families, people with mental health issues, and those with low levels of education.ConclusionsStronger evidence supports the effects of geographic and demographic variables on youth and adolescent suicide rates as compared with spatial variables. These findings suggest that policy makers take spatial and demographic factors into consideration when health systems allocate resources for suicide prevention, and that national policymakers integrate demographic and geographic variables into health service programs.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023430994.
A survey of mental health literacy in parents and guardians of teenagers
Authors: Kusaka Sakurako , Foo Jerome Clifford , Yamaguchi Satoshi , Yukawa Ayuko , Arai Takuya , Sawamura Fumika , Togo Fumiharu , Sasaki Tsukasa 
Published: February 2024

IntroductionParents and guardians (hereafter caregivers) of teenagers need high levels of mental health literacy (MHL) to manage mental health problems arising in teenagers in their care. Previous studies assessing MHL levels in caregivers of teenagers have reported mixed results, making it difficult to clearly estimate caregiver MHL levels. This study aimed to investigate MHL levels in Japanese caregivers of regular teenagers.MethodsResponses from caregivers (n = 1,397) of students entering junior and senior high schools to a self-administered online questionnaire were analyzed. The questionnaire assessed (a) knowledge about mental health/illnesses and (b) attitudes towards mental health problems in teens in their care (e.g., recognition of depression as a medical illness and intention to engage in helping behaviors).ResultsThe average proportion of correct answers to the knowledge questions (n = 7) was 55.4%; about one tenth (9.2%) of caregivers correctly answered only one or none of the questions. Few caregivers correctly answered about the life-time prevalence of any mental illnesses (46.1%) and appropriate sleep duration for teenagers’ health (16.5%). The proportions of caregivers who had the intention to listen to the teen in their care, consult another person, and seek professional medical help if the teen suffered from depression were 99.5%, 91.5% and 72.7%, respectively.ConclusionsMany teenagers’ caregivers appeared to be willing to help the teens in their care if they were suffering from mental health problems. However, there was much room for improvement in knowledge on mental health/illnesses and intention to seek help from medical professionals. Efforts toward better education should be made.
A three-player game model for promoting enterprise green technology innovation from the perspective of media coverage
Authors: Liu Yan , Chen Yun-ping , Xie Tong-ping , Xia Yi-han 
Published: February 2024

ObjectiveThe objective of this study was to explore the game relationship among enterprise, the government, and the public under the new media environment, so as to provide decision-making reference for improving enterprise green technology innovation and promoting economy high-quality development with new media participation.MethodsThis study constructs a three-subject evolutionary game model of enterprise, government, and public based on multi-agent relationship analysis and evolutionary game theory. In addition, the derivation of an evolutionary equilibrium strategy and numerical simulation analysis is carried out to comprehensively explore the evolution trajectory of green technology innovation system under the new media environment.Findings(1) The system may have four stable evolutionary strategies: (1,0,0), (0,0,1), (1,0,1), and (1,1,1). (2) The initial strategy probability of various actors would affect the system evolution speed but not the evolution result, and the authenticity of new media reports is an important factor determining the system evolution of green technology innovation. (3) Numerical simulation results show that a fair and just new media environment can effectively constrain the traditional production behavior of enterprise, actively guide the public to participate in supervision, and play an alternative role to government regulation to a certain extent.ValueThis study explores the evolutionary balance strategy of green technology innovation system under the new media environment, which not only enriches relevant theories of media environment governance but also has important reference value for promoting enterprises’ green technology innovation and establishing an environmental governance system jointly governed by government, enterprise, public, and media.
Evaluation of a point-of-use device used for autoantibody analysis and its potential for following microcystin leucine-arginine exposure
Authors: Ma Hui , Loscher Christine , Parle-McDermott Anne , Fitzgerald Jenny , Meneely Julie , Elliott Christopher , Welten Richard , Mchau Geofrey J. , Makule Edna , Machunda Revocatus , Gong Yun Yun , Kimanya Martin , Crawley Aoife , Maguire Ivan , Murphy Caroline , O’Kennedy Richard 
Published: February 2024

Introduction: Globally, the need for measuring exposure to algal toxins has become urgent due to ever-increasing reports of contamination in sea and freshwater, in shellfish and fish stocks and in aerosols.Methods: To address this issue, we evaluated the potential of determining autoantibodies to a panel of biomarkers known to be elevated following exposure to the hepatotoxin microcystin leucine-arginine. The presence of autoantibodies, specific to four selected stress-response, metabolomic and chaperone biomarkers, namely, Heat shock protein 1, Triosephosphate isomerase, Peroxiredoxin 1 and Peroxiredoxin 2 was employed in screening 371 serum samples from microcystin-exposed individuals in Tanzania. In addition, the capacity of the LightDeck fluorescence-based detector, a point-of-use device, to monitor these autoantibody responses in comparison to enzyme-linked immunosorbent assay was evaluated.Results: By using the determination of autoantibodies to this novel panel of biomarkers an altered response was observed following microcystin exposure, with levels generally upregulated. The presence of elevated levels of microcystin leucine-arginine in water, as well as in food sources in Tanzania, may potentially have significant health effects on the population.Discussion: This novel biomarker panel may have potential for the detection of microcystin leucine-arginine exposure as well as various microcystin exposure-associated cancers (e.g., hepatocellular cancer and colorectal cancer). In addition, the utilisation of the LightDeck point-of-use device proved successful for the rapid analysis of this biomarker panel.