bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isoquercetin for thromboinflammation in sickle cell disease: a randomized double-blind placebo-controlled trial
Authors: Lizarralde-Iragorri Maria A. , Parachalil Gopalan Bindu , Merriweather Brenda , Brooks Jennifer , Hill Mai , Lovins Dianna , Pierre-Charles Ruth , Cullinane Ann , Dulau-Florea Alina , Lee Duck-Yeon , Villasmil Rafael , Jeffries Neal , Shet Arun S. 
Published: December 2023

Abstract
Data from a small trial in patients with cancer suggest that isoquercetin (IQ) treatment lowered thrombosis biomarkers and prevented clinical thrombosis, but, to our knowledge, no studies of IQ have been conducted to target thromboinflammation in adults with sickle cell disease (SCD). We conducted a randomized, double-blind, placebo-controlled trial in adults with steady-state SCD (hemoglobin SS [HbSS], HbSβ0thal, HbSβ+thal, or HbSC). The primary outcome was the change in plasma soluble P-selectin (sP-selectin) after treatment compared with baseline, analyzed in the intention-to-treat population. Between November 2019 and July 2022, 46 patients (aged 40 ± 11 years, 56% female, 75% under hydroxyurea treatment) were randomized to receive IQ (n = 23) or placebo (n = 23). IQ was well tolerated and all the adverse events (AEs; n = 21) or serious AEs (n = 14) recorded were not attributable to the study drug. The mean posttreatment change for sP-selectin showed no significant difference between the treatment groups (IQ, 0.10 ± 6.53 vs placebo, 0.74 ± 4.54; P = .64). In patients treated with IQ, whole-blood coagulation (P = .03) and collagen-induced platelet aggregation (P = .03) were significantly reduced from the baseline. Inducible mononuclear cell tissue factor gene expression and plasma protein disulfide isomerase reductase activity were also significantly inhibited (P = .003 and P = .02, respectively). Short-term fixed-dose IQ in patients with SCD was safe with no off-target bleeding and was associated with changes from the baseline in the appropriate direction for several biomarkers of thromboinflammation. The trial was registered at www.clinicaltrials.gov as #NCT04514510.
High Population Frequency of GNRHR p.Q106R in Malta: An Evaluation of Fertility and Hormone Profiles in Heterozygotes
Authors: Axiak Clayton John , Pleven Adrian , Attard Ritienne , Borg Carbott Francesca , Ebejer Jean-Paul , Brincat Ian , Cassar Karen , Gruppetta Mark , Vassallo Josanne , Bezzina Wettinger Stephanie , Farrugia Rosienne 
Published: December 2023

Abstract

Context
The gonadotropin-releasing hormone receptor variant GNRHR p.Q106R (rs104893836) in homozygosity, compound heterozygosity, or single heterozygosity is often reported as the causative variant in idiopathic hypogonadotropic hypogonadism (IHH) patients with GnRH deficiency. Genotyping of a Maltese newborn cord-blood collection yielded a minor allele frequency (MAF) 10 times higher (MAF = 0.029; n = 493) than that of the global population (MAF = 0.003).

Objective
To determine whether GNRHR p.Q106R in heterozygosity influences profiles of endogenous hormones belonging to the hypothalamic-pituitary axis and the onset of puberty and fertility in adult men (n = 739) and women (n = 239).

Design, Setting, and Participants
Analysis of questionnaire data relating to puberty and fertility, genotyping of the GNRHR p.Q106R variant, and hormone profiling of a highly phenotyped Maltese adult cohort from the Maltese Acute Myocardial Infarction Study.

Main Outcome and Results
Out of 978 adults, 43 GNRHR p.Q106R heterozygotes (26 men and 17 women) were identified. Hormone levels and fertility for all heterozygotes are within normal parameters except for TSH, which was lower in men 50 years or older.

Conclusion
Hormone data and baseline fertility characteristics of GNRHR p.Q106R heterozygotes are comparable to those of homozygous wild-type individuals who have no reproductive problems. The heterozygous genotype alone does not impair the levels of investigated gonadotropins and sex steroid hormones or affect fertility. GNRHR p.Q106R heterozygotes who exhibit IHH characteristics must have at least another variant, probably in a different IHH gene, that drives pathogenicity. We also conclude that GNRHR p.Q106R is likely a founder variant due to its overrepresentation and prevalence in the island population of Malta.
Visual Metaphors: On the Linguistic Structure of Hybrid Creatures in Art
Authors: Hagberg Garry L. 
Published: December 2023

Abstract
Metaphors make possible creative and personally expressive ways of describing the world in a way that exceeds blunt literal description. In this article, the author considers (1) the ways metaphors function, (2) the ways that connotation, association, and implication can enrich and inflect the meanings of our words as we use them, and, finally, (3) the significance that these issues concerning verbal or linguistic meaning hold for our comprehension of parallel forms of meaning in the visual arts. The emphasis is on the artistic representation of hybrid creatures in painting, because where metaphors lead us to see one thing in the light of the other, so with hybrid creatures we can see either or any part of the hybrid in the light of the other part or parts. With some of the similarities between metaphorical speech and visual perception and interpretation identified, I turn to works by Caravaggio, Fuseli, the Parthenon sculptures, Botticelli, Picasso, William Blake, and Hieronymus Bosch.
Mixed Reality for Cranial Neurosurgical Planning: A Single-Center Applicability Study With the First 107 Subsequent Holograms
Authors: Colombo Elisa , Regli Luca , Esposito Giuseppe , Germans Menno R. , Fierstra Jorn , Serra Carlo , Sebök Martina , van Doormaal Tristan 
Published: December 2023

BACKGROUND AND OBJECTIVES:
Mixed reality (MxR) benefits neurosurgery by improving anatomic visualization, surgical planning and training. We aim to validate the usability of a dedicated certified system for this purpose.

METHODS:
All cases prepared with MxR in our center in 2022 were prospectively collected. Holographic rendering was achieved using an incorporated fully automatic algorithm in the MxR application, combined with contrast-based semiautomatic rendering and/or manual segmentation where necessary. Hologram segmentation times were documented. Visualization during surgical preparation (defined as the interval between finalized anesthesiological induction and sterile draping) was performed using MxR glasses and direct streaming to a side screen. Surgical preparation times were compared with a matched historical cohort of 2021. Modifications of the surgical approach after 3-dimensional (3D) visualization were noted. Usability was assessed by evaluating 7 neurosurgeons with more than 3 months of experience with the system using a Usefulness, Satisfaction and Ease of use (USE) questionnaire.

RESULTS:
One hundred-seven neurosurgical cases prepared with a 3D hologram were collected. Surgical indications were oncologic (63/107, 59%), cerebrovascular (27/107, 25%), and carotid endarterectomy (17/107, 16%). Mean hologram segmentation time was 39.4 ± 20.4 minutes. Average surgical preparation time was 48.0 ± 17.3 minutes for MxR cases vs 52 ± 17 minutes in the matched 2021 cohort without MxR (mean difference 4, 95% CI 1.7527-9.7527). Based on the 3D hologram, the surgical approach was modified in 3 cases. Good usability was found by 57% of the users.

CONCLUSION:
The perioperative use of 3D holograms improved direct anatomic visualization while not significantly increasing intraoperative surgical preparation time. Usability of the system was adequate. Further technological development is necessary to improve the automatic algorithms and reduce the preparation time by circumventing manual and semiautomatic segmentation. Future studies should focus on quantifying the potential benefits in teaching, training, and the impact on surgical and functional outcomes.
BBQ methods: streamlined workflows for bacterial burden quantification in infected cells by confocal microscopy
Authors: Augenstreich Jacques , Shuster Michael , Fan Yongqiang , Lyu Zhihui , Ling Jiqiang , Briken Volker 
Published: December 2023

ABSTRACT
Accurate quantification of bacterial burden within macrophages, termed bacterial burden quantification (BBQ), is crucial for understanding host–pathogen interactions. Various methods have been employed, each with strengths and weaknesses. This article addresses limitations in existing techniques and introduces two novel, automated methods for BBQ within macrophages based on confocal microscopy data analysis. The first method refines total fluorescence quantification by incorporating filtering steps to exclude uninfected cells, while the second method calculates total bacterial volume per cell to mitigate potential biases in fluorescence-based readouts. These workflows utilize PyImageJ and Cellpose software, providing reliable, unbiased, and rapid quantification of bacterial load. The proposed workflows were validated using Salmonella enterica serovar Typhimurium and Mycobacterium tuberculosis models, demonstrating their effectiveness in accurately assessing bacterial burden. These automated workflows offer valuable tools for studying bacterial interactions within host cells and provide insights for various research applications.
Role of antenatal care in reducing the risk of postpartum acute kidney injury
Authors: Afzal Aurangzeb , Saleem Sidra , Anjum Roshina , Tayyab Haleema 
Published: December 2023

Objective: Antenatal visits play a very important role to diagnose and manage pregnancy related health issues. This study was an attempt to identify the reasons that increase the risk of postpartum acute kidney Injury with special focus on antenatal care.
Methods: We analyzed 110 patients in Nephrology and Gynaecology wards in Lahore General Hospital. Out of these 40 had Postpartum Acute Kidney Injury and 70 patients did not have it. Questionnaire regarding aspects of antenatal care (demographics, timing and number of antenatal visits) was filled by the patient or immediate family members.
Results: Mean age of the 110 patients was 26.45 years. Mean Duration of pregnancy in the control group was 36.12 weeks and in cases it was 31.62 weeks. Out of 110 patients, 36(32.72%) patients did not have any antenatal visit while 62(56.3%) patients had more than five visits. Out of the 40 Postpartum Acute Kidney Injury patients, 23(57.5%) patients did not get any antenatal care. Out of 70 patients without Postpartum Acute Kidney Injury, 13 did not have any antenatal care. There were 19 patients who did not have booked visits because of financial Issues, followed by lack of awareness in 12 patients, distance issues for three patients and lack of family support for two patients.
Conclusion: Patients who did not have antenatal care were at an increased risk of developing PPAKI. Financial issues and lack of awareness were the most common risk factors for compromised antenatal care.
doi: https://doi.org/10.12669/pjms.40.3.7127
How to cite this: Afzal A, Saleem S, Anjum R, Tayyab H. Role of antenatal care in reducing the risk of postpartum acute kidney injury. Pak J Med Sci. 2024;40(3):505-508. doi: https://doi.org/10.12669/pjms.40.3.7127
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
MYTHOLOGICAL DISCOURSE AS SUGGESTIVE TYPE OF COMMUNICATION
Authors: Voronova Natal'ya 
Published: December 2023

The article describes mythological discourse as a suggestive type of communication.
The theoretical and methodological basis of the study was the structural-functionalist methodology, which made it possible to identify the role of mythological discourse in the modern communication space and its impact on mass consciousness. Structuralist, hermeneutic, semiotic and phenomenological methods have become important for revealing the suggestive mechanisms of modern myth-making.
In the process of research, the functions of modern mythological discourse were identified: warning, prognostic, modification, projective, manipulative, mediative, integrative, incentive, simulation, regulatory, suggestive-explanatory.
The author has studied the characteristic features, technologies and goals of the suggestive influence of mythological discourse on mass consciousness.
Civilization and culture in the XXI century. Dialogue, convergence or confrontation? (communication aspect)
Authors: Didenko Valeriy , Timerbaev R. 
Published: December 2023

. It seems to us that although each of the concrete historical forms of culture functions within "its" civilization, genetically, structurally and functionally, art always retains its spiritual and creative "redundancy" in relation to its civilization, and its signs precisely characterize culture in relation to civilization from the side of its relative autonomy and creativity freedom.
The orientation of civilization towards the means of life, the immediate practical interests and values due to its focus on the development, use of natural conditions of life, external life are complemented in any particular society, "enlightened" by the appeal of culture, and especially art to internal life, expressing the boundlessness of the "claims of the human spirit".
Layers of psychic reality are available for artistic reflection. And it is no coincidence, apparently, that in scientific research on this issue we sometimes see more references to the works of artists than to the actual scientific works. The same thing happens with objective reality, and on the borders of the unknown, the infinite. The requirement of reliability, verifiability, and the necessary axiomatization of knowledge complicates the formation and institutionalization of a hypothetical idea in science, requires overcoming the existing paradigm and corresponding empirical confirmations. Otherwise, the unknown is explicated in art. It enters into it in the full scope of uncertainty and improbability.
If technical civilization builds its relations with the natural universe, realizing the principles of consumption and subjugation of nature, subordination to man, as if for the benefit of man, then culture, and above all art, cultivate our attitude to nature, which is based on disinterested contemplation, reverence, reverent admiration as the highest and most perfect form of creation, which is for a person, an eternal source of creative inspiration. Unlike the anthropocentrism of civilization, art is characterized by environmentalism and the desire for universal unity with nature, its pantheistic perception.
The Phenomenon of Liturgical Texts in Russian: Hermeneutical Analysis of Selected Passages Intercessio Anaphora of St. Basil the Great
Authors: Cyrel'chuk K. 
Published: December 2023

Introduction. At the present stage, a corpus of liturgical texts in Russian has been formed, which requires a comprehensive analysis within the hermeneutical direction. Thus, the methods of hermeneutic analysis, which is interdisciplinary in nature, can be applied in the analysis of liturgical texts in Russian.
Hermeneutics of liturgical texts is the interpretation of a liturgical (liturgical) text from the point of view of its theological, historical, philological and other contexts and liturgical use in order to establish its true meaning and an accurate understanding of its content.
Aim. The purpose of this study is to conduct a hermeneutic analysis of the chosen place of the anaphora of St. Basil the Great in Russian.
Methods. descriptive, comparative, hermeneutical analysis methods.
Results. The hermeneutic analysis of the part of the anaphora of St. Basil the Great in Russian is presented, which allows us to identify the interdisciplinary nature of this humanitarian field. At the same time, the proposed methodology allows applying a similar approach to other liturgical texts in Russian.
Scientific novelty. The translation of Orthodox worship, being an act of intercultural communication, affects several linguistic and cultural traditions simultaneously: Greek Byzantine, Church Slavonic and Russian. Hermeneutical analysis of such texts in Russian requires special knowledge from these contexts.
Practical significance. The results of this study allow for a comprehensive hermeneutic analysis of other liturgical texts in Russian.
Communicative Valence of Hedonistic Values in the Traditional Russian Axiosphere
Authors: Bochina T. 
Published: December 2023

The relevance of the research is conditioned by the undoubted scientific significance of cognition of structural and semantic features of axiosphere and types of interaction between different values that make up the space of Russian linguoculture.
The aim of the article is to reconstruct a fragment of the Russian axiosphere with the top "happiness" by means of representation of interrelations of this value with others. At the same time, the task of establishing the communicative axiological valence of hedonistic values is solved. The subject of the study is the types of relations of different values with each other, the object of the study is Russian proverbs and sayings about happiness / unhappiness.
The main methods of research are axiological analysis, semantic, descriptive-analytical methods.
Results. It is proved that in Russian proverbs the value "happiness" interacts with different axiological types: moral and moral, psychological, intellectual and cognitive, religious, existential, utilitarian and practical, economic and other values. Thus, the axiological valence of hedonistic values is multi-place. The following types of axiological associations have been identified: harmonious combination of different values/anti-values, contrast of value and anti-value of one kind, contradictory combination of values and anti-values of different kinds.
The scientific novelty consists in introducing into scientific circulation the concept of communicative axiological valence, which is understood as the ability of lexemes naming this or that value to enter into semantic and syntactic relations with other axiolexemes in an utterance.
The practical significance of the article is that the proposed approach to the value aspect of communication can be used in the study of the axiological picture of the world of the Russian people, as well as in the development of university special courses on axiology and paremiology.
Ophthalmic examination in children – comprehensive literature review
Authors: Pactwa Filip , Ślusarczyk Daniel , Żmuda Bartłomiej , Jakubowska Wiktoria , Pisera Piotr , Kiełkowicz Aleksandra , Żuberek Michał , Popińska Zuzanna 
Published: December 2023

The proper functioning of the patient in the environment is made possible by the interaction of individual systems or single organs. The visual system is undoubtedly one of the most important; it is the one that enables us to find our way and acquire a range of skills to survive in the environment. Unfortunately, if neglected at a young age, complications may remain for the rest of life. A number of tests have now been introduced to check the development and efficiency of a child's eyesight; their cyclical repetition and appropriate identification of changes or lack of them enable an appropriate diagnosis to be made and treatment to be applied, thanks to which we can eliminate or reduce the defect and, more importantly, prevent the development of a new one.
Contrary to appearances, vision screening in children does not take place in a single medical visit, it is an ongoing process, and the activities included in the screening should be carried out at each follow-up visit. This allows the practitioner to detect risk factors and eye abnormalities early on, which increases the chance of a positive outcome for the patient. It also enables the specialist to identify children who require more frequent eye checks, or patients who should be referred to a specialist qualified in children's eye examination.
During the screening examination, the doctor begins by talking to the child and parent about conditions that run in the family. The aim is to detect and identify risk factors that need to be carefully analysed. These factors include, but are not limited to, premature birth, Down's syndrome, cerebral palsy, a family history of strabismus, visual impairment, retinoblastoma, childhood glaucoma, childhood cataract or eye disease and genetic systemic diseases.
Taenia solium excretory secretory proteins (ESPs) suppresses TLR4/AKT mediated ROS formation in human macrophages via hsa-miR-125
Authors: Arora Naina , Keshri Anand K. , Kaur Rimanpreet , Rawat Suraj S. , Kumar Rajiv , Mishra Amit , Prasad Amit 
Published: December 2023

Background
Helminth infections are a global health menace affecting 24% of the world population. They continue to increase global disease burden as their unclear pathology imposes serious challenges to patient management. Neurocysticercosis is classified as neglected tropical disease and is caused by larvae of helminthic cestode Taenia solium. The larvae infect humans and localize in central nervous system and cause NCC; a leading etiological agent of acquired epilepsy in the developing world. The parasite has an intricate antigenic make-up and causes active immune suppression in the residing host. It communicates with the host via its secretome which is complex mixture of proteins also called excretory secretory products (ESPs). Understanding the ESPs interaction with host can identify therapeutic intervention hot spots. In our research, we studied the effect of T. solium ESPs on human macrophages and investigated the post-translation switch involved in its immunopathogenesis.

Methodology
T. solium cysts were cultured in vitro to get ESPs and used for treating human macrophages. These macrophages were studied for cellular signaling and miR expression and quantification at transcript and protein level.

Conclusion
We found that T. solium cyst ESPs treatment to human macrophages leads to activation of Th2 immune response. A complex cytokine expression by macrophages was also observed with both Th1 and Th2 cytokines in milieu. But, at the same time ESPs modulated the macrophage function by altering the host miR expression as seen with altered ROS activity, apoptosis and phagocytosis. This leads to activated yet compromised functional macrophages, which provides a niche to support parasite survival. Thus T. solium secretome induces Th2 phenomenon in macrophages which may promote parasite’s survival and delay their recognition by host immune system.