bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cervical cytological studies in women inserted with Norplant‐I contraceptive
Authors: Misra Jata S. , Tandon Prabha , Srivastava Aarti , Das Kalyani , Chandrawati  , Saxena Nirakar C. 
Published: September 2003

AbstractThe study was carried out by the Human Reproduction Research Centre of the Indian Council of Medical Research at Lucknow and Kanpur, India as part of a multicenter study to ascertain the oncogenic potential of Norplant‐I, a progestational contraceptive, by periodic cytological evaluation of cervical smears. In total, 278 fertile healthy women were inserted with Norplant‐I at the Contraceptive Clinic of the Department of Obstetrics and Gynaecology of K.G.'s Medical College, Lucknow and G.S.V.M. Medical College, Kanpur, were and followed for periods ranging from 1–5 yr. It was reassuring to note that squamous intraepithelial lesion (SIL) incidence was found to be significantly higher only in the initial stages of contraception after 1 yr of Norplant insertion, after which it declined; no SIL was seen after 3 yr of contraception. The retrogression of SIL was also seen in all 11 pre‐ and 15 of 18 postinsertional cases. As SIL incidence showed a rise with increasing age and parity, the study also highlighted the need for constraint in offering Norplants to women of high age and parity. The percentage of inflammatory smears were also high till 4 yr of Norplant use, warranting a need for adequate treatment and early repeat cytology to exclude the persistence of inflammation. The accumulated data indicate the safety of long‐term use of Norplants upto 5 yr, as far as oncogenic potential is concerned. Diagn. Cytopathol. 2003;29:136–139. © 2003 Wiley‐Liss, Inc.
Loa loa presenting in a ThinPrep® Pap test™: Case report and review of parasites in cervicovaginal cytology specimens
Authors: Stelow Edward B. , Pambuccian Stefan E. , Bardales Ricardo H. , Cartwright Charles P. , Reif Christopher J. , Stanley Michael W. 
Published: September 2003

AbstractParasites other than Trichomonas vaginalis may occasionally present in Pap tests obtained during gynecologic examination. We present a case of Loa loa found on a Pap test from an apparently healthy 19‐yr‐old woman who had immigrated to the US at the age of 15 from Cameroon. We discuss the cytologic features from this case and then briefly review Loa loa and the presence of parasites in Pap tests and other cervicovaginal specimens. Diagn. Cytopathol. 2003;29:167–171. © 2003 Wiley‐Liss, Inc.
Role of HPV DNA testing in predicting cervical intraepithelial lesions: Comparison of HC HPV and ISH HPV
Authors: Qureshi M. Nasar , Rudelli Raoul D. , Tubbs Raymond R. , Biscotti Charles V. , Layfield Lester J. 
Published: September 2003

AbstractHuman papillomavirus (HPV) is widely accepted as the primary agent involved in the development of squamous intraepithelial neoplasia and cervical carcinoma. Several commercial tests are available for detecting HPV DNA. This study compares the efficacy of INFORM®HPV (in situ hybridization [ISH] HPV) and HCII (HC HPV) in predicting cervical lesions. A total of 762 sequential Papanicolaou (Pap) smears determined by cytologic examination to be either atypical squamous cells of undetermined significance (ASC‐US) or low‐grade squamous intraepithelial lesions (LSIL) were tested by both Hybrid Capture (HC) HPV and ISH HPV; 250 follow‐up biopsies were reviewed as the reference standard for presence or absence of a lesion. ISH HPV and HC HPV differed significantly in accurately predicting biopsy findings from ASC‐US and LSIL cases. The overall sensitivity and specificity of ISH HPV were 97% (28/29) and 86% (191/221); and HC HPV was 79% (23/29) and 56% (123/221). The positive predictive value (PPV) of ISH HPV was 48% (28/58) vs HC HPV value of 19% (23/121). Negative predictive value (NPV) was also better with ISH HPV at 99% (191/192) and HC HPV at 95% (123/129). Of equal importance, ISH HPV demonstrated a lower false‐positive rate compared to HC HPV, 12% (30/250) vs 39% (98/250), as well as having a slightly lower false‐negative rate 0.4% (1/250) vs 2.4% (6/250). ISH HPV is more predictive of biopsy histopathology in patients with detectable cervical lesions than is HC HPV. Effective triage of patients by HPV analysis using ISH HPV as compared to HC HPV has the potential of significant public health impact by reducing unnecessary colposcopies, as well as adverse medical, social, and psychological patient consequences. Diagn. Cytopathol. 2003;29:149–155. © 2003 Wiley‐Liss, Inc.
Leiomyosarcoma of the breast: A difficult diagnosis on fine‐needle aspiration biopsy
Authors: Jun Wei Xiao , Hiotis Karen , Garcia Roberto , Hummel Levine Pascale 
Published: September 2003

AbstractLeiomyosarcoma of the breast is rarely encountered in fine‐needle aspiration (FNA) cytologic material. We report a case of primary leiomyosarcoma of the breast in a 52‐yr‐old female. Aspiration cytology showed large, dissociated round to spindle cells with abundant vacuolated cytoplasm, pleomorphic nuclei, prominent nucleoli, and occasional intranuclear cytoplasmic invaginations. Mitotic figures, osteoclast‐like giant cells, and stromal fragments were identified. A diagnosis of malignant neoplasm representing either a sarcoma, a poorly differentiated carcinoma, or a metaplastic carcinoma was made. The patient underwent a wide excision of the lesion after negative work‐up. Histologic examination and immunohistochemical studies established the diagnosis of leiomyosarcoma. This case is presented here because we feel that, although FNA cytology with eventual ancillary studies is a valuable diagnostic tool to evaluate any breast mass, malignant spindle cell neoplasms of the breast still represent a diagnostic challenge for the cytopathologist. Recognition of all cytologic features of leiomyosarcoma may help to formulate a correct diagnosis. Diagn. Cytopathol. 2003;29:172–178. © 2003 Wiley‐Liss, Inc.
Chemical composition variability of the essential oil of Conyza canadensis Cronq.
Authors: Lis Anna , Piggott John R. , Góra Józef 
Published: September 2003

AbstractThe essential oils from different botanical parts (herb, leaves, ﬂowers, stems, roots) of Conyza canadensis Cronq. were analysed at various ontogenesis phases. R‐(+)‐limonene and trans‐α‐bergamotene were the main constituents of the herb, leaf and ﬂower oil at all phases of vegetation. The early ﬂowering phases are the best time for harvesting C. canadensis herb, because the oil yield is the highest (0.7–0.8%) and its chemical composition is constant (limonene 80–81%, trans‐α‐bergamotene 6–8%). Limonene was the dominating compound of the oils of European origin (French, Italian, Spanish, Belgian, Bulgarian and Lithuanian). Copyright © 2003 John Wiley & Sons, Ltd.
Chemical composition of fruit and stem essential oils of Lantana camara from northern India
Authors: Khan M. , Srivastava S. K. , Jain Neetu , Syamasundar K. V. , Yadav Anju K. 
Published: September 2003

AbstractThe chemical composition of fruits and stem essential oils of Lantana camara from the northern plains of India were analysed by GC and GC–MS, which resulted in the identiﬁcation of 52 and 66 constituents, representing 98.1% and 96.6% of the oils, respectively. The major constituents in the fruits oil were palmitic acid (22.8%), stearic acid (12.8%) and germacrene‐D (7.1%), while the major constituents in the stem oil were palmitic acid (32.7%) and stearic acid (23.9%). Copyright © 2003 John Wiley & Sons, Ltd.
Antimicrobial activity of essential oils from Tunisian aromatic plants
Authors: Bouzouita Nabiha , Kachouri Faten , Hamdi Moktar , Chaabouni Mohamed Moncef 
Published: September 2003

AbstractThe essential oils from Thymus capitatus (L.) Hoffmgg. et Link, Ocimum basilicum (L.), Myrtus communis (M.) and Laurus nobilis (L.) have been characterized and tested against two bacteria (Lactobacillus plantarum and Escherichia coli) and a fungus (Geotrichum candidum), using a submerged broth culture method. The results obtained showed that T. capitatus and O. basilicum were the greatest inhibitors of all the strains tested, and that Escherichia coli was more inhibited than Lactobacillus plantarum by all the essential oils tested. Copyright ©2003 John Wiley & Sons, Ltd.
Chemical composition of the leaf volatile oils of Callitris intratropica R. T. Baker & H. G. Smith from Nigeria
Authors: Ogunwande Isiaka A. , Olawore Nureni O. , Kasali Adeleke A. , König Wilfried A. 
Published: September 2003

AbstractThe chemical composition of the leaf volatile oils of Callitris intratropica from two different regions in Nigeria were investigated by GC and GC–MS. The essential oils comprised mainly monoterpenoids (93.0–95.9%), while sesquiterpenoids constituted the minor components (1.4–4.4%). The quantitatively signiﬁcant constituents of the essential oils were α‐pinene (35.9–55.6%), limonene (21.6–50.5%) and myrcene (6.0–10.1%). Copyright © 2003 John Wiley & Sons, Ltd.
Volatile components from the roots of Scrophularia ningpoensis Hemsl.
Authors: Miyazawa Mitsuo , Okuno Yoshiharu 
Published: September 2003

AbstractThe composition of the volatile oil from the radix of Scrophularia ningpoensis has been investigated by capillary GC, GC–MS. The oil contained 103 volatile components, of which 3.49% were terpenoids. The main constituents were fatty acids, such as palmitic acid (25.40%), linoleic acid (10.04%), α‐linolenic acid (6.06%), γ‐linolenic acid (4.82%), cis‐palmitoleic acid (3.94%), trans‐oleic acid (3.52%), cis‐oleic acid (2.67%) and trans‐palmitoleic acid (1.20%). Copyright © 2003 John Wiley & Sons, Ltd.
Secretory elements of needles and berries of Juniperus communis L. ssp. communis and its volatile constituents
Authors: Shahmir F. , Ahmadi L. , Mirza M. , Korori S. A. A. 
Published: September 2003

AbstractThe structure of the secretory elements and the essential oils of the needles and berries of Juniperus communis L. ssp. communis growing in north Iran were studied. The needles, which contain ducts and transfusion tissue, act as conduction structures, and the berries containing elongate tubercles which act as reservoirs of volatile oils. The essential oils, isolated by hydrodistillation, were analysed by GC–MS. The oil contained 41 and 27 components in the needles and berries, respectively. The needle oil consisted mainly of sabinene (40.7%), α‐pinene (12.5%) and terpinen‐4‐ol (12.3%) and the berry oil included sabinene (36.8%), α‐pinene (20%), limonene (10.6%), germacrene D (8.2%) and myrcene (4.8%) as the main components. Copyright © 2003 John Wiley & Sons, Ltd.
Enones of (+)‐ and (−)‐3‐pinanone: inﬂuence of chirality on ﬂavour
Authors: Buchbauer G. , Shaﬁi‐Tabatabai A. 
Published: September 2003

AbstractStarting with (+)‐ and (−)‐3‐pinanone [(+)‐ and (−)‐2], the enones 3–12 were prepared by aldol condensation with various aldehydes. Without isolating the corresponding β‐hydroxy ketones, the target compounds 3–12 could be obtained in good to moderate yields directly from the starting bicyclic ketone. The odour properties of the enones were evaluated and described. It could be shown that the structure of the aldehyde as well as the chirality is crucial for the ﬂavour impression of the enone. However, the (+)‐enantiomers generally possess more agreeable odours than their antipodes. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oil composition of Aloysia polystachya (Griseb.) Moldenke under rust disease
Authors: Cabanillas Carmen M. , Lopez María L. , Daniele Graciela , Zygadlo Julio A. 
Published: September 2003

AbstractProspodium lippiae (Speg.) Arth.‐Berth (Basidiomycota, Heterobasidiomycetes, Uropyxidaceae) affects essential oil composition in Aloysia polystachya (Griseb.) Moldenke. Infected plants increased their content of carvone to 72.0%, while decreasing their percentage of α‐thujone to zero. Copyright © 2003 John Wiley & Sons, Ltd.
Characteristic odour components of Citrus inflata Hort. ex Tanaka (Mochiyu) cold‐pressed peel oil
Authors: Minh Tu N. T. , Onishi Y. , Son U. S. , Ogawa E. , Ukeda H. , Sawamura M. 
Published: September 2003

AbstractCharacteristic odour components of Citrus inflata Hort. ex Tanaka (Mochiyu) were studied by sensorial and instrumental techniques. Flavour dilution (FD) factor and relative flavour activity (RFA) of individual volatile components were evaluated by aroma extraction dilution analysis (AEDA) and gas chromatography–olfactometry (GC–O). The results indicated that hydrocarbons (98.1%) made up most of the oil: monoterpenes and sesquiterpenes were 97.6% and 0.5%, respectively. Among oxygenated compounds, linalool (0.2%) as an alcohol, perillaldehyde (0.1%) and tridecanal (0.1%) as aldehydes showed relatively high quantities, while the others were all less than 0.05%. AEDA and GC–O indicated that myrcene, (E)‐ocimene, cis‐linalool oxide, trans‐linalool oxide, β‐copaene, perillaldehyde and perillyl alcohol were the odour characteristic compounds, showing Mochiyulike odour. Careful sniff testing revealed that (E)‐ocimene could be an key odour compound of Mochiyu aroma. Copyright © 2003 John Wiley & Sons, Ltd.
Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells
Authors: Velkey J. Matthew , O'Shea K. Sue 
Published: September 2003

AbstractSummary: We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem‐loop RNA transcripts corresponding to Oct4 mRNA. Cells transfected with Oct4 RNAi constructs demonstrated reduced levels of Oct4 mRNA and exhibited characteristics of trophectodermal differentiation. These findings support the critical role of Oct4 in regulating stem cell identity and suggest that future experiments using RNAi in ES cells can elucidate the roles of other genes affecting lineage specification during differentiation. genesis 37:18–24, 2003. © 2003 Wiley‐Liss, Inc.
RNA inhibition of BMP‐4 gene expression in postimplantation mouse embryos
Authors: Gratsch Theresa E. , De Boer Lisa S. , O'Shea K. Sue 
Published: September 2003

AbstractShort, hairpin RNA (shRNA) directed against bone morphogenetic protein 4 (Bmp‐4) was delivered to early postimplantation staged mouse embryos via tail vein injection of pregnant dams. As early as 24 h postinjection, embryos expressed a DsRed marker and later exhibited defects of neural fold elevation and closure and of cardiac morphogenesis. Immunohistochemical analysis of sectioned embryos indicated that Bmp‐4 protein was depleted and gene expression analysis indicated there was a reduction in Bmp‐4 mRNA and an upregulation of the Bmp‐4 antagonists, noggin and chordin, in embryos exposed to the shRNA, but not in control embryos. There was no change in the expression of Gata4, brachyury, or claudin6 in RNAi exposed embryos, indicating that RNA silencing was specific to Bmp‐4 rather than producing widespread gene inhibition. Delivery of shRNA to embryos has the potential to specifically knockdown the expression of developmentally essential genes and to rescue gene mutations, significantly decreasing the time required to analyze the function(s) of individual genes in development. genesis 37:12–17, 2003. © 2003 Wiley‐Liss, Inc.
α Complementation in the Cre recombinase enzyme
Authors: Casanova Emilio , Lemberger Thomas , Fehsenfeld Sandra , Mantamadiotis Theo , Schütz Günther 
Published: September 2003

AbstractSummary: The Cre‐loxP system is increasingly exploited for spatial and temporal gene inactivation. Here we present a novel approach to achieve this goal of selective gene inactivation. Following the model of α complementation in the β‐galactosidase enzyme, where the enzyme is split into independent polypeptides which are able to associate and maintain the enzymatic activity, we divided the Cre recombinase into two independent polypeptides (one containing the NH2 terminus (α) and a second one containing the COOH‐terminus (β)). Individually, the two polypeptides have no detectable activity. However, when coexpressed the polypeptides are able to associate, giving rise to Cre enzymatic activity, which optimally is as high as 30% of that seen with wildtype Cre recombinase in vitro. We present this strategy as a modification of the traditional Cre‐loxP system, which could be used to obtain a highly specific recombination pattern by expressing the two halves under the control of separate promoters. genesis 37:25–29, 2003. © 2003 Wiley‐Liss, Inc.
Involvement of nuclear phosphatidylinositol‐dependent phospholipases c in cell cycle progression during rat liver regeneration
Authors: Albi Elisabetta , Rossi Graziella , Maraldi Nadir Mario , Magni Mariapia Viola , Cataldi Samuela , Solimando Liliana , Zini Nicoletta 
Published: September 2003

AbstractNuclear lipid metabolism is involved in the regulation of cell proliferation. Modulation of the expression and activity of nuclear PI‐phospholipase C (PI‐PLC) has been reported during liver regeneration after partial hepatectomy, although it has not been determined whether different PLC isoforms play specific roles in the regulation of cell cycle progression. Here, we report evidence that the increased activity of nuclear PLCs in regenerating rat liver occurs before the peak of DNA replication and involves the enzyme activity associated to the chromatin and not that associated to the nuclear membrane. Immunocytochemical analyses indicate that PI‐PLC β1 isoform is exclusively localized at the chromatin level, PI‐PLC β1 co‐localizes with DNA replication sites much more than PI‐PLC γ1, which is also present at the nuclear envelope. These findings and the increased amount of PI‐PLC γ1 occurring after the peak of DNA replication suggest that PI‐PLC β1 and γ1 play different roles in cell cycle progression during regenerating liver. The increased activity of PI‐PLC β1 constitutively present within the hepatocyte nucleus, should trigger DNA replication, whereas PI‐PLC γ1 should be involved in G2/M phase transition through lamin phosphorylation. J. Cell. Physiol. 197: 181–188, 2003. © 2003 Wiley‐Liss, Inc.
Synaptic vesicle morphology and recycling are altered in myenteric neurons of mice lacking dystrophin (mdx mice)
Authors: Vannucchi Maria Giuliana , Corsani Letizia , Faussone‐Pellegrini Maria‐Simonetta 
Published: September 2003

AbstractSeveral dystrophin isoforms are known. The full‐length isoform is present in striated and smooth muscles and neurons and its lack causes Duchenne Muscular Dystrophy, a progressive myopathy accompanied by mild cognitive deficits and gastrointestinal dismotility. An ultrastructural study was undertaken in the colon of mice lacking full‐length dystrophin and maintaining shorter isoforms (mdx mice) to ascertain whether myenteric neurons have an altered morphology. Results showed a significant increase in the size of synaptic vesicle and in the number of recycling vesicles. An enlargement of endoplasmic reticulum cisternae in a subpopulation of neurons was also seen. Immunohistochemistry confirmed that the shorter isoforms were expressed in mdx mice myenteric neurons. These findings indicate the presence of a neuropathy at the myenteric plexus which might justify the defective neuronal control of gastrointestinal motility reported for these animals and which might be correlated with full‐length dystrophin loss, since the shorter isoforms are present. J. Cell. Physiol. 197: 232–242, 2003. © 2003 Wiley‐Liss, Inc.
NF1 modulates the effects of ras oncogenes: Evidence of other NF1 function besides its GAP activity
Authors: Corral Teresa , Jiménez María , Hernández‐Muñoz Inmaculada , Castro Ignacio Pérez de , Pellicer Angel 
Published: September 2003

AbstractNeurofibromin (NF1) (the product of Nf1 gene) is a large cytosolic protein known as a negative regulator of Ras. A fragment of some 400 residues located at the center of the NF1 GAP‐Related Domain (NF1‐GRD) has strong identity with other molecules of the GAP family, which comprises, among others, the mammalian proteins NF1 and p120GAP, and the yeast proteins IRA1 and IRA2. GAP family members are known by their ability to promote the GTPase activity of Ras proteins, facilitating the transit of those proteins to their inactive state. Recent findings (Tong et al., 2002, Nat Neurosci 5:95–96) indicate that NF1 may be involved in the regulation of adenyl cyclase activity. Our results show that NF1‐GRD cooperates with Ras in the anchorage‐independent growth capacity of Ras‐expressing fibroblasts, without affecting: (i) their ability to grow in low serum, (ii) their cellular adhesion capability, or (iii) the expression of key proteins involved in cell–cell and cell–matrix interactions. On the other hand, NF1 overexpression induces an increase in the expression levels of the focal adhesion kinase (FAK), and specific changes in the activation status of the mitogen‐activated protein kinases (MAPKs). These results suggest the existence of a Ras‐independent NF1‐dependent pathway able to modify the levels of expression of FAK and the levels of activation of MAPKs. Because FAK and many proteins recently found to bind NF1 have a role in the cytoskeleton, this pathway may involve rearrangement of cytoskeletal components that facilitate anchorage independence. J. Cell. Physiol. 197: 214–224, 2003. © 2003 Wiley‐Liss, Inc.
Decapeptide with fibroblast growth factor (FGF)‐5 partial sequence inhibits hair growth suppressing activity of FGF‐5
Authors: Ito Chikako , Saitoh Yuko , Fujita Yasuko , Yamazaki Yoshimitsu , Imamura Toru , Oka Syuichi , Suzuki Satoshi 
Published: September 2003

AbstractEarlier studies demonstrated that knock‐out of fibroblast growth factor‐5 gene (Fgf‐5) prolonged anagen VI phase of hair cycle, resulting long hairs in the mice. We showed the activities on hair growth of the two Fgf‐5 gene products, one of which, FGF‐5 suppressed hair growth by inhibiting anagen proceeding and inducing the transition from anagen to catagen, and FGF‐5S, a shorter polypeptide with FGF‐5‐antagonizing activity translated from alternatively spliced mRNA, suppressed this activity of FGF‐5. As the results suggested that FGF‐5 antagonist would increase hair growth, we synthesized various peptides having partial sequences of human FGF‐5 and FGF‐5S and determined their FGF‐5 antagonist activity. Among them, a decapeptide designated P3 (95‐VGIGFHLQIY‐104) that aligns with receptor binding sites of FGF‐1 and FGF‐2 suppressed FGF‐5‐induced proliferation of BALB/3T3 A31 and NIH/3T3 murine fibroblasts, and FGF receptor‐1c (FGFR‐1c)‐transfected Ba/F3 cell line (FR‐Ba/F3 cells). IC50s of this peptide on these cell proliferations were 64, 28, 146 μM, respectively. On the other hand, IC50 of this peptide on binding of FGF‐5 to the FGFR‐1(IIIc)/Fc chimera was 483 μM. Examination in dorsal depilated mice revealed that the P3 peptide reduced the activity of FGF‐5 to recover hair pigmentation and hair follicle lengths. The classification of histologically observed skin sections showed FGF‐5‐induced delations of anagen procedure had reduced by the P3 peptide. The anti‐Ki67 antibody staining of hair follicles was inhibited by administration of FGF‐5, and this inhibition by FGF‐5 was recovered by administration of the P3 peptide. The P3 peptide alone did not affect hair follicle length and hair cell proliferation. These results indicate that the decapeptide antagonized FGF‐5 activity in vivo, and reduced the inhibition of FGF‐5 in hair growth, confirming that FGF‐5 inhibitors are promising substances against hair loss and/or for promoting hair growth. J. Cell. Physiol. 197: 272–283, 2003. © 2003 Wiley‐Liss, Inc.