bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting informed approaches in precision oncology and clinical trial participation for Black patients with cancer: Community‐engaged development and pilot testing of a digital intervention
Authors: An Jinghua , Ferrante Jeanne M. , Macenat Myneka , Ganesan Shridar , Hudson Shawna V. , Omene Coral , Garcia Harold , Kinney Anita Y. 
Published: October 2023

AbstractBackgroundBlack patients with cancer are less likely to receive precision cancer treatments than White patients and are underrepresented in clinical trials. To address these disparities, the study aimed to develop and pilot‐test a digital intervention to improve Black patients’ knowledge about precision oncology and clinical trials, empower patients to increase relevant discussion, and promote informed decision‐making.MethodsA community‐engaged approach, including a Community Advisory Board and two rounds of key informant interviews with Black patients with cancer, their relatives, and providers (n = 48) was used to develop and refine the multimedia digital intervention. Thematic analysis was conducted for qualitative data. The intervention was then pilot‐tested with 30 Black patients with cancer to assess feasibility, acceptability, appropriateness, knowledge, decision self‐efficacy, and patient empowerment; Wilcoxon matched pairs signed‐rank test was used to analyze quantitative data.ResultsThe digital tool was found to be feasible, acceptable, and culturally appropriate. Key informants shared their preferences and recommendations for the digital intervention and helped improve cultural appropriateness through user and usability testing. In the pilot test, appreciable improvement was found in participants’ knowledge about precision oncology (z = –2.04, p = .052), knowledge about clinical trials (z = –3.14, p = .001), and decisional self‐efficacy for targeted/immune therapy (z = –1.96, p = .0495).ConclusionsThe digital intervention could be a promising interactive decision‐support tool for increasing Black patients’ participation in clinical trials and receipt of precision treatments, including immunotherapy. Its use in clinical practice may reduce disparities in oncology care and research.Plain Language Summary

We developed a digital interactive decision support tool for Black patients with cancer by convening a Community Advisory Board and conducting interviews with Black patients with cancer, their relatives, and providers.
We then pilot‐tested the intervention with newly diagnosed Black patients with cancer and found appreciable improvement in participants’ knowledge about precision oncology, knowledge about clinical trials, and confidence in making decisions for targeted/immune therapy.
Our digital tool has great potential to be an affordable and scalable solution for empowering and educating Black patients with cancer to help them make informed decisions about precision oncology and clinical trials and ultimately reducing racial disparities.
Iridium Catalyst Immobilized on Crosslinked Polyethyleneimine for Continuous Hydrogen Production Using Formic Acid
Authors: Sawahara Keito , Tanaka Shinji , Kodaira Tetsuya , Kanega Ryoichi , Kawanami Hajime 
Published: October 2023

AbstractHydrogen is an alternative fuel that can play a critical role in achieving net zero emissions, leading to global environment sustainability. An iridium‐immobilized catalyst based on polyethyleneimine (PEI) was synthesized and utilized for hydrogen production via formic acid dehydrogenation (FADH). Iridium complex is cross‐linked with its ligand and PEI to form the immobilized catalyst, where the iridium content could be easily varied in the range of 1–10 %. The structure of the iridium‐immobilized catalyst was confirmed using solid‐state NMR, DNP NMR, and FTIR spectroscopies. The iridium‐immobilized catalyst with PEI showed excellent catalytic activity for FADH, exhibiting the catalyst's highest turnover frequency (TOF) value of 73 200 h−1 and a large turnover number (TON) value of over 1 130 000. The catalyst could be used for continuous hydrogen production via FADH, exhibiting high durability for over 2 000 h with TON value of 332 889 without any degradation in catalytic activity. The obtained hydrogen gas was evaluated for power generation using a standard fuel cell, as well as achieved 5 h of stable power generation.
Cullin‐associated and neddylation‐dissociated 1 regulate reprogramming of lipid metabolism through SKP1‐Cullin‐1‐F‐boxFBXO11‐mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma
Authors: Zhang Hao , Xia Peng , Yang Zhangshuo , Liu Jie , Zhu Yimin , Huang Zan , Zhang Zhonglin , Yuan Yufeng 
Published: October 2023

AbstractBackgroundEnhanced de novo lipogenesis is essential for hepatocellular carcinoma (HCC). Abnormally high cullin‐associated and neddylation‐dissociated 1 (CAND1) expression is associated with poor clinical prognosis in HCC. The SKP1‐Cullin‐1‐F‐box (SCF) complex consists of the SKP1, Cullin‐1 and F‐box proteins (FBPs) and performs multiple functions including adipogenesis. SCF complex was modulated by CAND1, but Whether and how the CAND1 promotes HCC by regulating SCF complex and lipogenesis are unknown.MethodsHCC samples were used to analyze the correlations between CAND1 expression and clinicopathological characteristics such as survival and prognosis. The in vitro functions of CAND1, FBXO11 and heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) were measured by cell proliferation, colony formation and migration assays. The in vivo functions were tested in multiple mouse liver cancer models including patient‐derived xenograft (PDX), cell line‐derived xenograft and AKT/NRASV12‐induced primary liver cancer models. Injections of adeno‐associated virus targeting CAND1 (AAV‐shCAND1) were performed to evaluate the therapeutic efficacy of targeting CAND1. RNA‐Seq and lipidomic assays followed by serial biochemical experiments including mass spectrometry, immunoprecipitation and GST pull‐down were performed to dissect the underlying mechanisms.ResultsCAND1 promoted the expression of lipid synthesis genes by disrupting SCF complex assembly and lipid accumulation. Furthermore, we identified hnRNPA2B1 as a novel F‐box protein 11 (FBXO11)‐binding partner. FBXO11 directly bound to hnRNPA2B1 and promoted hnRNPA2B1 ubiquitination and subsequent degradation. Our evaluations of the therapeutic efficacy of AAV‐shCAND1 injections confirmed that targeting the CAND1‐SCFFBXO11‐hnRNPA2B1A signalling axis was therapeutically effective. CAND1 downregulation significantly reduced the tumour burden in a primary mouse liver cancer model and a PDX model.ConclusionsOur results highlight that CAND1 is associated with poor prognosis in HCC and regulates lipid metabolic reprogramming by dissociating the SCF complex. Targeting the CAND1‐SCFFBXO11‐hnRNPA2B1 axis may be a novel strategy for HCC treatment.
H1Innovative approaches to combat anti‐cancer drug resistance: Targeting lncRNA and autophagy
Authors: Zhong Chenming , Xie Zijun , Duan Shiwei 
Published: October 2023

AbstractBackgroundTo date, standardizing clinical predictive biomarkers for assessing the response to immunotherapy remains challenging due to variations in personal genetic signatures, tumour microenvironment complexities and epigenetic onco‐mechanisms.Main BodyEarly monitoring of key non‐coding RNA (ncRNA) biomarkers may help in predicting the clinical efficacy of cancer immunotherapy and come up with standard predictive ncRNA biomarkers. For instance, reduced miR‐125b‐5p level in the plasma of non‐small cell lung cancer patients treated with anti‐PD‐1 predicts a positive outcome. The level of miR‐153 in the plasma of colorectal cancer patients treated with chimeric antigen receptor T lymphocyte (CAR‐T) cell therapy may indicate the activation of T‐cell killing activity. miR‐148a‐3p and miR‐375 levels may forecast favourable responses to CAR‐T‐cell therapy in B‐cell acute lymphoblastic leukaemia. In cancer patients treated with the GPC3 peptide vaccine, serum levels of miR‐1228‐5p, miR‐193a‐5p and miR‐375‐3p were reported as predictive biomarkers of good response and improved overall survival. Therefore, there is a critical need for further studies to elaborate on the key ncRNA biomarkers that have the potential to predict early clinical responses to immunotherapy.ConclusionsThis review summarises important predictive ncRNA biomarkers that were reported in cancer patients treated with different immunotherapeutic modalities including monoclonal antibodies, small molecule inhibitors, cancer vaccines and CAR‐T cells. In addition, a concise discussion on forthcoming perspectives is provided, outlining technical approaches for the optimal utilisation of immune‐modulatory ncRNA biomarkers as predictive tools and therapeutic targets.
Darwin, Haeckel, and the “Mikluskan gas organ theory”
Authors: Werneburg Ingmar , Hoßfeld Uwe , Levit Georgy S. 
Published: October 2023

AbstractA previously unknown reference to the Russian ethnologist, biologist, and traveler Nikolai N. Miklucho‐Maclay (1846–1888) was discovered in correspondence between Charles Darwin (1809–1882) and Ernst Haeckel (1834–1919). This reference has remained unknown to science, even to Miklucho‐Maclay's biographers, probably because Darwin used the Russian nickname “Mikluska” when alluding to this young scientist. Here, we briefly outline the story behind the short discussion between Darwin and his German counterpart Haeckel, and highlight its importance for the history of science. Miklucho‐Maclay's discovery of a putative swim bladder anlage in sharks, published in 1867, was discussed in four letters between the great biologists. Whereas, Haeckel showed enthusiasm for the finding because it supported (his view on) evolutionary theory, Darwin was less interested, which highlights the conceptual differences between the two authorities. We discuss the scientific treatment of Miklucho‐Maclay's observation in the literature and discuss the homology, origin, and destiny of gas organs—swim bladders and lungs—in vertebrate evolution, from an ontogenetic point of view. We show that the conclusions reached by Miklucho‐Maclay and Haeckel were rather exaggerated, although they gave rise to fundamental insights, and we illustrate how tree‐thinking may lead to differences in the conceptualization of evolutionary change.
Recent advances on portable photoelectrochemical biosensors for diagnostics
Authors: Wu Di , Tang Dianping 
Published: October 2023

AbstractEfficient and accurate diagnostic methods are important for human health monitoring and prevention of major diseases, so the establishment of efficient and convenient testing methods for clinical diagnosis is urgent. Photoelectrochemical (PEC) biosensors have attracted much attention because of their high sensitivity, low detection background, and especially the advantages of combining photoexcitation and electrochemical detection. With the rapid development of integrated circuit technology many wireless transmissions of highly aggregated portable micro devices are manufactured, its small size, easy to carry, and the ability to visualize the results of the test is shown to be the potential for the application of such devices. This review aims to cover recent advances in PEC biosensing within the field of portable detection, including the principles of PEC biosensors, common photovoltaic materials, and scalable fabrication techniques for portable PEC biosensing. Future prospects in the field are also discussed.
Effects of tea application on cadmium, phosphorus, and iron accumulations in rice (Oryza sativa L.) seedlings
Authors: Shi Jun , Hu Rongping , Li Qin , Li Xiufen , Liu Dingyou , Xiang Zufen , Liao Shuang , Yu Huaqing , Huang Tingyou , Li Yan 
Published: October 2023

AbstractTo decrease the cadmium (Cd) uptake in rice and study the relationships of phosphorus (P), iron (Fe), and Cd uptakes in rice, the effects of four different tea types (green, black, dark, and white teas) on the P, Fe, and Cd accumulation in rice seedlings were studied. The results showed that only the black and dark teas increased the biomass of rice seedlings. The application of green, black, dark, and white teas increased the photosynthetic pigment content to some extent. Only the black tea increased the shoot P content, and the black, dark, and white teas increased the shoot Fe content. Compared with the control, only the black and dark teas decreased the shoot Cd content by 9.51% and 25.02%. Correlation and gray relational analyses showed that the root Cd content, soil pH value and soil exchangeable Cd concentration were the most closely associated with the shoot Cd content. The principal component and cluster analyses also showed the top three close cluster categories with shoot Cd content were root Cd content, soil exchangeable Cd concentration, and soil pH value. These findings indicate that the application of black and dark teas can promote the growth and decrease the Cd uptake of rice.
Spatial transcriptomic analysis of the mouse brain following chronic social defeat stress
Authors: Wang Ting , Song Zhihong , Zhao Xin , Wu Yan , Wu Liying , Haghparast Abbas , Wu Haitao 
Published: October 2023

AbstractDepression is a highly prevalent and disabling mental disorder, involving numerous genetic changes that are associated with abnormal functions in multiple regions of the brain. However, there is little transcriptomic‐wide characterization of chronic social defeat stress (CSDS) to comprehensively compare the transcriptional changes in multiple brain regions. Spatial transcriptomics (ST) was used to reveal the spatial difference of gene expression in the control, resilient (RES) and susceptible (SUS) mouse brains, and annotated eight anatomical brain regions and six cell types. The gene expression profiles uncovered that CSDS leads to gene synchrony changes in different brain regions. Then it was identified that inhibitory neurons and synaptic functions in multiple regions were primarily affected by CSDS. The brain regions Hippocampus (HIP), Isocortex, and Amygdala (AMY) present more pronounced transcriptional changes in genes associated with depressive psychiatric disorders than other regions. Signalling communication between these three brain regions may play a critical role in susceptibility to CSDS. Taken together, this study provides important new insights into CSDS susceptibility at the ST level, which offers a new approach for understanding and treating depression.
A comprehensive review of goji berry processing and utilization
Authors: Yu Jing , Yan Yamei , Zhang Lutao , Mi Jia , Yu Limei , Zhang Fengfeng , Lu Lu , Luo Qing , Li Xiaoying , Zhou Xuan , Cao Youlong 
Published: October 2023

AbstractGoji berry (wolfberry, Lycium), is a genus of Solanaceae, in which the roots, stems, leaves, and fruits are for both food and medicinal uses. In recent years, the demand for health food and research purposes has led to increasing attention being paid to the application of goji berry nutrients and resources. There are three general strategies to process and utilize the goji berry plant. First, the primary processing of goji berry products, such as dried goji berry pulp, and fruit wine with its by‐products. Second, deep processing of sugar–peptides, carotenoids, and the extraction of other efficacy components with their by‐products. Third, the utilization of plant‐based by‐products (roots, stems, leaves, flowers, and fruit residuals). However, the comprehensive use of goji berry is hampered by the non‐standardized production technology of resource utilization and the absence of a multi‐level co‐production and processing technology systems. On the basis of this, we review some novel techniques that are made to more effectively use the resources found in goji berry or its by‐products in order to serve as a guide for the thorough use of these resources and the high‐quality growth of the goji berry processing industry.
Phytate degradation in composite wheat/cassava/sorghum bread: Effects of phytase‐secreting yeasts and addition of yeast extracts
Authors: Vilanculos Serafina Lídia , Svanberg Ulf , Andlid Thomas 
Published: October 2023

AbstractIron deficiency anemia is highly prevalent in developing countries due to the consumption of cereal‐based foods rich in phytate that chelates minerals such as iron and zinc making them unavailable for absorption by humans. The aim of the present study was to degrade phytic acid in composite flour (wheat/cassava/sorghum) bread by the addition of phytase‐producing yeasts in the baking process to achieve a phytate‐to‐iron molar ratio <1 and a phytate‐to‐zinc molar ratio <15, ratios needed to achieve an enhanced absorption by humans. The high‐phytase (HP)‐producing yeasts were two Saccharomyces cerevisiae (YD80 and BY80) that have been genetically modified by a directed mutagenesis strategy, and Pichia kudriavzevii TY13 isolated from a Tanzanian lactic fermented maize gruel (togwa) and selected as naturally HP yeast. To further improve the phytase production by the yeasts, four different brands of phytase‐promoting yeast extracts were added in the baking process. In addition, two yeast varieties were preincubated for 1 h at 30°C to initiate phytase biosynthesis. The phytate content was measured by high‐performance ion chromatography (HPIC) and the mineral content by ion chromatography (HPIC). The results showed that all three HP yeasts improved the phytate degradation compared with the composite bread with no added HP yeast. The composite bread with preincubated S. cerevisiae BY80 or P. kudriavzevii TY13 plus Bacto yeast extract resulted in the lowest phytate content (0.08 μmol/g), which means a 99% reduction compared with the phytate content in the composite flour. With added yeast extracts from three of the four yeast extract brands in the baking process, all composite breads had a phytate reduction after 2‐h fermentation corresponding to a phytate: iron molar ratio between 1.0 and 0.3 and a phytate: zinc molar ratio <3 suggesting a much‐enhanced bioavailability of these minerals.
Nutritional analysis of rice landraces from southern Odisha, India
Authors: Panda Koustava Kumar , Bisht Satpal Singh , Mishra Rojita , Sahu Parmeshwar Kumar , Panda Amrita Kumari , Subedi Roshan 
Published: October 2023

AbstractRice landraces conserved by tribal farmers are important for their nutritional richness. Landraces are rich in essential amino acids, vitamins, anthocyanins, and flavonoids useful to cure noncommunicable diseases and metabolic disorders. A study was carried out with 10 rice landraces from the tribal‐dominated belt of Southern Odisha to investigate grain nutrition, proximate composition, and vitamin and mineral contents. The protein content of the landraces was higher (>6 g/100 g) and the fat content was lower (<0.6 g/100 g) than popular Indian rice varieties. The mean nutrient content of 10 rice landraces was as follows: protein 6.3 ± 0.313 g/100 g, total dietary fiber 1.6 ± 0.094 g/100 g, fat 0.536 ± 0.008 g/100 g, ash 10.514 ± 6.753%, and total sugar 77.18 ± 2.118 g/100 g. The high genotypic coefficient of variation (GCV) was observed for alkali spreading value (31.11%), capacity of grain hydration (52.705), index of hydration (171.439), moisture (46.343%), and vitamin B2 (23.994%) in rice landraces. Few landraces had superior iron content: Kalamalli (1.49 mg/100 g), Kandulakathi (1.42 mg/100 g), and Dudhamani (1.39 mg/100 g) compared to popular Indian rice varieties. Tikichudi had highest moisture (19%) and fat (0.53 g/100 g) content, which signifies the taste quality of rice. Kanakchudi exhibited the highest fiber content (1.8 g/100 g) and ash content (22.80%). Kalamalli contained higher zinc (0.49 mg/100 g), iron (1.49 mg/100 g), potassium (108.33 mg/100 g), magnesium (78.33 mg/100 g), and phosphorus (125.00 mg/100 g), whereas Muktabali was found to have higher Ca (3.88 mg/100 g) and Baunsidubraj exhibited higher niacin (4.9 mg/100 g). The indigenous landraces Kalamalli, Kandulakathi, and Dudhamani had considerably high iron content, whereas Kalamalli, Baunsidubraj, and Muktabali possessed less phytic acid in comparison with existing varieties and other landraces reported from various states of our country. Landraces Kalamalli, Kanakchudi, Tikichudi, and Muktabali from southern Odisha, India, represented nutritionally better genetic pool for future rice improvement.
Aberrant degree centrality profiles during rumination in major depressive disorder
Authors: Jia Feng‐Nan , Chen Xiao , Du Xiang‐Dong , Tang Zhen , Ma Xiao‐Yun , Ning Tian‐Tian , Zou Si‐Yun , Zuo Shang‐Fu , Li Hui‐Xian , Cui Shi‐Xian , Deng Zhao‐Yu , Fu Jia‐Lin , Fu Xiao‐Qian , Huang Yue‐Xiang , Li Xue‐Ying , Lian Tao , Liao Yi‐Fan , Liu Li‐Li , Lu Bin , Wang Yan , Wang Yu‐Wei , Wang Zi‐Han , Ye Gang , Zhang Xin‐Zhu , Zhu Hong‐Liang , Quan Chuan‐Sheng , Sun Hong‐Yan , Yan Chao‐Gan , Liu Yan‐Song 
Published: October 2023

AbstractRumination is closely linked to the onset and maintenance of major depressive disorder (MDD). Prior neuroimaging studies have identified the association between self‐reported rumination trait and the functional coupling among a network of brain regions using resting‐state functional magnetic resonance imaging (MRI). However, little is known about the underlying neural circuitry mechanism during active rumination in MDD. Degree centrality (DC) is a simple metric to denote network integration, which is critical for higher‐order psychological processes such as rumination. During an MRI scan, individuals with MDD (N = 45) and healthy controls (HC, N = 46) completed a rumination state task. We examined the interaction effect between the group (MDD vs. HC) and condition (rumination vs. distraction) on vertex‐wise DC. We further characterized the identified brain region's functional involvement with Neurosynth and BrainMap. Network‐wise seed‐based functional connectivity (FC) analysis was also conducted for the identified region of interest. Finally, exploratory correlation analysis was conducted between the identified region of interest's network FCs and self‐reported in‐scanner affect levels. We found that a left superior frontal gyrus (SFG) region, generally overlapped with the frontal eye field, showed a significant interaction effect. Further analysis revealed its involvement with executive functions. FCs between this region, the frontoparietal, and the dorsal attention network (DAN) also showed significant interaction effects. Furthermore, its FC to DAN during distraction showed a marginally significant negative association with in‐scanner affect level at the baseline. Our results implicated an essential role of the left SFG in the rumination's underlying neural circuitry mechanism in MDD and provided novel evidence for the conceptualization of rumination in terms of impaired executive control.