bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial Diabetes Mellitus with Mitochondrial DNA 3316G&gt;A Mutation: A Unique Autopsy Case Presenting with Sepsis-Associated Cholestasis
Authors: Mashiba Chikara , Shioya Akihiro , Kumagai Motona , Hoshi Daisuke , Yoshida Mitsuteru , Shimasaki Miyako , Oyama Takeru , Shirakawa Tomohiko , Yamada Sohsuke 
Published: October 2023

Abstract
Background: We describe a unique autopsy case of mitochondrial diabetes mellitus (MDM) with mitochondrial DNA 3316G>A mutation, in which, a characteristic sepsis-associated cholestasis was finally recognized via the pathological examinations, and the clinical diagnosis of the cause of jaundice with conjugated hyperbilirubinemiawas very difficult. Case Presentation: A 74-year-old Japanese female was diagnosed with MDM showing a point mutation at mitochondrial DNA 3316G>A on the age of 38. She had been treated with hemodialysis due to the diabetic nephropathy and end-stage renal failure since the age of 66. She noticed urinary tract infection and diabetic gangrene of foot with Gram-negative Bacteroides fragilis bacteremia, followed by aggressive jaundice with high serum level of direct bilirubin, and died 2 months after the symptom onset. At autopsy, multiple foci of bacteremia-induced hemorrhagic infarction were observed in the congestive bilateral lungs, whereas the cholestatic liver revealed no overt gross cholangiectasis. Microscopic findings characteristically showed many bile thrombi in the biliary canaliculi of hepatic lobules without any evidence of severe shock liver. Finally, we diagnosed exclusively and conclusively as sepsis-associated cholestasis due to the marked elevation of Gram-negative bacteria-derived endotoxins and inflammatory cytokines.
Conclusion: We propose that these unique liver features in our MDM case might be one of new clues to unveil its enigmatic etiology. Further prospective studies are needed to validate the presence and significance of sepsis-associated cholestasis, after collecting and investigating a larger number of MDM cases with mitochondrial DNA 3316 mutation examined.
Patients With Age-related Macular Degeneration Lack Knowledge and Self-management Skills of Age-related
Authors: Yuqian Ren , Haiyan Luo , Liu Qiong , Meidan Wen , Tao Ling 
Published: October 2023

Abstract
Objective:To understand the status quo and factors influencing self-management behavior in patients with age-related macular degeneration, provide evidence for better self-management and develop self-management evaluation tools.
Methods:A lot of 17 patients with age-related macular degeneration were interviewed using semi-structured questionnaires. The phenomenological research method was applied, and the data were collated according to Colaizzi's 7-step phenomenological analysis method.
Results: The status quo and factors influencing self-management behavior in patients with age-related macular degeneration could be summarized into five themes:1）serious daily dysfunction caused by visual impairment and inability to cope with methods and skills; 2）lack of disease cognition, timely treatment, and poor compliance; 3）the presence of underlying diseases and poor management of comorbidity; 4）lack of disease health knowledge and display of bad habits and rebuilding healthy behavior motivation; 5）negative emotion .
Conclusion: Patients with age-related macular degeneration have poor self-management knowledge and self-management ability. There is a need to strengthen the thematic popular science education of age-related macular degeneration at the social level, learn from advanced countries, collaborate with medical association and communities, and actively promote the development and application of visual rehabilitation training, social psychological intervention and self-management projects.Taken together these approaches could improve the self-management ability of patients with AMD.
The combination of doxazosin and metyrosine as a preoperative treatment for pheochromocytomas and paragangliomas
Authors: Ohmachi Yuka , Yamamoto Masaaki , Inaba Yuiko , Makino Shohei , Urai Shin , Matsumoto Risa , Bando Hironori , Kanie Keitaro , Tsujimoto Yasutaka , Motomura Yuma , Sasaki Yuriko , Oi Yuka , Yamamoto Naoki , Suzuki Masaki , Takahashi Michiko , Iguchi Genzo , Kanzawa Maki , Furukawa Junya , Shigemura Katsumi , Mizobuchi Satoshi , Ogawa Wataru , Fukuoka Hidenori 
Published: October 2023

Abstract
Purpose: Preoperative medical management is critical to prevent intraoperative cardiovascular complications in patients with pheochromocytomas and paragangliomas (PPGLs). Initial treatment involves α-adrenergic receptor blockers. However, while the routine use of metyrosine alongside these blockers is not strongly recommended due to a lack of evidence supporting its efficacy and associated safety concerns, there are previous studies on combination therapy with phenoxybenzamine and metyrosine. There are few reports on combination therapy with the selective α1-adrenergic receptor blocker, doxazosin. Therefore, we investigated this combination treatment, which theoretically can affect perioperative outcomes in patients with PPGLs. To our knowledge, this is the first such study.
Methods: This retrospective single-center observational study involved 51 patients who underwent surgical resection of PPGLs at Kobe University Hospital between 2014 and 2022. All patients received doxazosin at maximum doses. Fourteen patients received concomitant metyrosine, while 37 received doxazosin alone. Their perioperative outcomes were compared.
Results: No severe event, such as acute coronary syndrome, was observed in either group. Intraoperatively, the doxazosin + metyrosine group exhibited a lower median minimum systolic blood pressure (56 [54-60] vs. 68 [59-74] mmHg, P = 0.03), and required lower median remifentanil (P = 0.04) and diltiazem (P = 0.02) doses than the doxazosin-alone group.
Conclusion: The combination of metyrosine and doxazosin as a preoperative treatment for PPGLs affects intraoperative circulatory hemodynamics, such as a reduced occurrence of blood pressure elevation during surgery. Further research is necessary to identify patients who will benefit most from this combination treatment.
Genome-wide investigation of UDP-Glycosyltransferase family in Tartary buckwheat (Fagopyrum tataricum)
Authors: Yang Fan , Zhang Lei , Zhang Xiao , Guan Jingru , Wang Bo , Wu Xiaoying , Song Minli , Wei Aili , Liu Zhang , Huo Dongao 
Published: October 2023

Abstract
Background: Tartary buckwheat (Fagopyrum tataricum) belongs to Polygonaceae family and has attracted increasing attention owing to its high nutritional value. UDP-glycosyltransferases (UGTs) glycosylate a variety of plant secondary metabolites to control many metabolic processes during plant growth and development. However, there have been no systematic reports of UGT superfamily in F. tataricum.
Results: We identified 173 FtUGTs in F. tataricum based on their conserved UDPGT domain. Phylogenetic analysis of these genes with 73 Arabidopsis UGTs clustered them into 21 families. FtUGTs from the same family usually had similar gene structure and motif compositions. Most of FtUGTs did not contain introns or had only one intron. Tandem repeats contributed more to FtUGTs amplification thansegmental duplications. Expression analysisindicates that FtUGTs are widely expressed in various tissues and likely play important roles in plant growth and development. The gene expression analysis response to different abiotic stresses showed that some FtUGTs were involved in response to drought and cadmium stress. Our study provides useful information on the UGTs in F. tataricum, and will facilitate their further study to better understand their function.
Conclusions: Our results provide a theoretical basis for further exploration of the functional characteristics of FtUGTs and for understanding the growth, development, and metabolic model in F. tataricum.
Do PET-positive supradiaphragmatic lymph nodes predict overall survival or the success of primary surgery in patients with advanced ovarian cancer?
Authors: Braun Christian , Grünig Hannes , Peikert Julia , Strobel Klaus , Christmann-Schmid Corina , Brambs Christine 
Published: October 2023

Abstract
Purpose:
Compared with conventional CT, FDG-PET/CT detects a higher rate of lymph node and distant metastases in ovarian cancer patients. However, it is not currently routinely performed in the pre-operative work-up. Therefore, we investigated the prognostic value of a pre-operative FDG-PET/CT in advanced epithelial ovarian cancer (EOC) and its predictive value regarding surgical resection to no residual disease. Specifically, a potential significance of PET-positive supradiaphragmatic lymph nodes (SDLN) on these parameters was evaluated.
Methods:
All patients with FIGO IIA-IVB EOC diagnosed between 03/2014–01/2021 at our certified gynecological cancer center with a FDG PET/CT prior to primary surgical therapy were retrospectively included.
Results:
A total of consecutive 53 patients were included in the study. 18 (34%) had PET-positive SDLN. We could not demonstrate a significant correlation between PET-positive SDLN and median overall survival (OS; SDLN positive: 58.76 months, SDLN negative: 60.76 months; p = 0.137) or intra- and perioperative outcomes.
Conclusion:
In ovarian cancer, FDG PET/CT has a higher detection rate for SDLN compared to CT detection rates described in the literature. PET-positive SDLNs failed to predict the intraoperative outcome or overall survival.
Changes in clinicopathological characteristics and surgical methods of adrenal tumors: high-volume institution experience of 1358 cases
Authors: Fu Weijin , Ma Chunlei , Yi Chufan , Zhang Mingjin , Lan Yibi 
Published: October 2023

Abstract
Background: The aim of this study to investigate the change trends in clinicopathological characteristics and surgical methods of adrenal tumors(AT) at a high-volume institution experience.
Methods: Data of AT patients who underwent adrenalectomy at our institution from January 2012 and December 2021 were retrospectively evaluated. The change trends of age, gender, nationality, tumor side, clinical manifestations, endocrine type,tumor diameter,pathological types and surgical methods indicators were analyzed between the two stages.
Result: A total of 1358 patients (mean age 46.2 years and diameter 3.38 cm) with underwent adrenalectomy were identified. There were 793 females and 565 males. Nationality include 905 han, 404 zhuang and 49 the other nationalities. There were 714 left side, 625 right side and 19 bilateral sides. Clinical manifestation included 914 clinical and 444 incidental types. Endocrine types included 729 function and 629 non-function.Histopathological diagnosiss included 1315 benign tumors (508 non-function adenomas, 298 primary aldosteronism, 215 pheochromocytomas,88 Cushing syndrome, 62 cyst,55 non-function nodular hyperplasia and 89 the other tumors) and 43 malignancies (17 adrenocortical cancer,9 metasta­sis and 17 the other tumors). The sum of the surgical types included 1059 RPLA,145 TPLA,106 OA and 48 RALA.
Conclusions: In our institution,there were more females than males.Simultaneously, Han were more than Zhuang nationalities. AT tended to occur on the left side, predominant clinical and functional types. Non-functional adenomas and ACC were the main pathological types of benign and malignant tumors, respectively. Although there were various surgical types for AT, RPLA was still the main surgical methods in our center. Keywords: Adrenal tumors, Clinicopathological characteristics, Surgical methods, Change trends
WITHDRAWN: Geoelectric Structure of Yalova-termal Town Bymagnetotelluric Data, Northwestern Turkey
Authors: Sevim Fatih , Pekşen Ertan , Dikbaş Aynur 
Published: October 2023

Abstract

In this study, the resistivity distribution of Termal town in the eastern Marmara region and its territory was investigated. Termal district is a seismically active region located between northern and southern branches of North Anatolian Fault in the Armutlu Peninsula. In the study area, magnetotelluric data were measured at 39 sounding locations. After the basic processing of collected MT data including the static shift correction, the resistivity structure of the corresponding area was obtained as a 3D earth model. Our aim is to obtain the underground conductivity structure of the study area, and to accomplish this, we utilize a well that produces hot water at a depth of 680 meters. The cross-sections in different directions obtained from the 3D earth model suggest that the study area is basically consisted of three distinguishable layers. The uppermost layer is a conductive layer and its thickness varies between 500 m and 1.5 km. 3D modelling of MT data infers a N-NW dipping major deep structure on the northern part of the Armutlu Peninsula. We propose that the north-dipping structure represents a tectonic contact between different tectonostratigraphic units exposed in the region. The results of this study and the geology of the region are compatible with each other. The result obtained from 3D MT, combined with the presence of a production well, confirms that the middle layer may serve as a potential aquifer for hot water in the surveyed area.
Antifungal Activity and Mechanism of Oxanthromicin against Verticillium dahliae
Authors: Wang Li-Jun , Wen Feng , Li Li-Xia , Xia Zhan-Feng 
Published: October 2023

Abstract
Oxanthromicin, an anthranone-type natural product isolated from Streptomyces sp. TRM 15522, exhibits high antifungal activity. However, the mechanisms underlying its antifungal activity remain unclear. Therefore, this study investigated its mode of action against the phytopathogen Verticillium dahlia. Oxanthromicin substantially suppressed spore germination and mycelial growth in V. dahlia. Electron microscopy and staining with propidium iodide and Rhodamine 123 indicated that oxanthromicin causes cell membrane damage and induces changes in mitochondrial membrane potential. These findings indicate that oxanthromicin exerts its fungicidal activity by disrupting the fungal membrane structure. This discovery could facilitate its development as a biological pesticide.
Gravity and trade in video on demand services
Authors: Studnicka Zuzanna , Broocks Annette 
Published: October 2023

Abstract

Over the last decade, watching videos online has become one of the primary uses of the internet, with streaming services accounting for more than 60% of global internet traffic. In this paper, we use a novel data set on Netflix, the largest streaming platform worldwide, to estimate the patterns of catalogue availability (extensive margin) and the number of clicks per title (intensive margin) across twenty countries. This data set also gives us a unique opportunity to estimate the importance of quality in viewing patterns. Our results show evidence of the gravity framework explaining both margins of Netflix watching. In addition, we find that there is a strong preference for domestic content, better-rated titles, and Netflix Original productions. These findings suggest that as Netflix produces more content, this will interact with its streaming dominance to provide a significant advantage in reaching viewers and promoting specific content.
JEL Classification: F10, L82, Z10
Human periodontal ligament stem cells (hPDLSCs) spontaneously differentiated into myofibroblast to repairer diabetic wounds
Authors: Li Yuxiao , Su Qi , Tao Zhaoyu , Cai Xiang , Zhao Yueping , Zhou Zhiying , Huang Yadong , Xiang Qi 
Published: October 2023

Abstract
Diabetic ulcer healing is affected by persistent hyperglycemia, and the current treatment methods are extremely limited. We aim to take the advantages of stem cell therapy and try to use periodontal ligament stem cells that have not yet become therapeutic cells to repair diabetic wounds, so as to provide theoretical basis for its application in clinical practice. Methods: The effect of AGEs on the myofibroblast differentiation ability of hPDLSCs was investigated by RT-qPCR and WBto detect the gene and protein expressions of α-SMA and COLIA1. HE staining and Masson staining were used to observe the effect of hPDLSCs on wound healing in STZ-induced diabetic mice, and immunohistochemical staining was used to detect the expression of α-SMA and CD31. Immunofluorescence (IF) was used to detect the expression of CD206 and iNOS in the tissue of wound area.hPDLSCs can spontaneously differentiate into myoblasts during culture. In α-MEM medium, at 25μg/mL AGEs, the expression of COLIA1 in the seventh passage (P7) cells was significantly decreased, and α-SMA was increased (P < 0.05). hPDLSCs hydrogel injection was used to heal the wounds of C57 mice, and it was found that the healing rate of hPDLSCs group was 65.247% on the 7th day of healing while that of the model group was 48.938%. The healing rate of hPDLSCs treatment group was much higher than that of the model group. Conclusion: hPDLSCs can repair the skin wounds of diabetic mice by spontaneously differentiating into myofibroblasts and resisting the damage of AGEs, which is expected to be used in clinical diagnosis and treatment.
Effect of gradation on Macro-meso shear properties of the alluvial- diluvial soil-rock mixture (ADSRM)
Authors: Liu Longqi , Fan Zhenhao , Yao Linhu , Yang Yunshu 
Published: October 2023

Abstract
Alluvial-diluvial soil-rock mixture (ADSRM) is a special geological material, mainly composed of pebbles, gravel, and soils. Gradation design has a remarkable effect on improving the shear strength of ADSRM. Therefore, the N-method of gradation theory was applied to the gradation design of ADSRM fillers, and the influence of gradation on macro-meso shear properties of ADSRM samples was analyzed. Macro-mechanical test results show that the shear strength, the equivalent cohesion, and the friction angle of the graded sample are much higher than that of the ungraded sample, and the shear dilatancy of the graded sample is more obvious. Meso-shear properties indicate that the percentage of pore area of the graded sample is less than that of the ungraded sample, while the percentage of the number of the small pore to the total pore and the crushed rock blocks under the same normal stress are more than that of the ungraded sample. Pore abundance shows the shape of most pores on the shear plane is oblate, secondly, near-equiaxed, and the least long-strip. Finally, the percentage of pore area decreases gradually with the increase of the shear displacement, while the percentage of crushed rock block area increases first and then decreases, which can reflect the change of shear strength at different shear stages. The number of pores and broken rock blocks mainly affect the strength and equivalent cohesion of the filler, while the abundance, roundness, shape coefficient, and fractal dimension of rock block contour mainly affect the internal friction angle.
Chemical bonding and valence states in Au-associated host minerals
Authors: Nzulu Gabriel , Magnusson Martin 
Published: October 2023

Abstract
Natural minerals play a vital role and exhibit unique electronic and structural properties that can be attributed to the presence of gold. Therefore, research on the chemical bonding of these materials is essential for contributing to better exploration, separation, and recovery techniques. The study of minerals from mining areas and their differences in correlation to Au contributes to an understanding of the chemical forms of invisible gold in rocks as well as their association with other mineral deposits. Here, we investigate the structural chemistry of gold (Au) and pathfinder elements in soil sediments (Au concentrates) containing host minerals of pyrite (FeS2), magnetite (Fe3O4), and quartz (SiO2) identified by X-ray diffraction (XRD). The samples were collected at an alluvial small-scale mining site and investigated by X-ray absorption near edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) at the Au 2p3/2 and Ag 1s absorption edges. XANES shows that the average valence states of Au have different weights of Au0, Au1+ and Au3+ depending on the composition of the host minerals at the mineral deposit. EXAFS shows that the interatomic Au-Au distances increase to 2.99–3.03 Å compared to 2.85 Å for Au metal, while the Ag-Ag distances increase to 2.93–2.97 Å compared to Ag metal of 2.86 Å. As shown in this work, XANES and EXAFS reveal the structural composition and chemical bonding of Au and Ag in host minerals that play an important role in the formation and stability of Au-associated mineral deposits. The results also reveal the alloying of Au with Ag and its association with S and O ligands in the host minerals as indistinguishable in X-ray diffraction. The knowledge will aid in mineral exploration and extraction based on geochemical data of the constituent minerals in potential geological zones.
Development of an online analyser to meet challenging new discharge limits for mercury in flue gas desulphurisation wastewater
Authors: Lancaster Shaun T. , Corns Warren T. , Krupp Eva M. , Feldmann Jörg 
Published: October 2023

Abstract

Anthropogenic emissions of mercury to the environment are of great concern due to its toxicity. The burning of coal contributes highly to Hg emissions and, as such, much lower effluent discharge limits for Hg in flue gas desulphurisation (FGD) wastewater have recently been proposed in the USA. Lower detection limits are required to meet the most demanding discharge limit of 10 ng L−1. Therefore, in this work, a new analyser for online monitoring of Hg in FGD wastewater based on syringe injections of chemicals combined with gold amalgamation – atomic fluorescence spectrometry has been developed. The instrument has been validated against international standard method ISO 17852, as well as an interference study with extreme levels of salts majorly present in FGD water. Detection limits of 1.8 ng L−1 as well as accurate performance in an on-site field trial indicate that the methodology is fit to meet the new legislation.
The Meaningful Function of the Emerging Clinical Targets-lncRNA MEG3 in
Gastric Cancer
Authors: Zhang Yi , Wang Yingchi , Jiang Yutong , Bai Huahui , Wen Yu 
Published: October 2023

Abstract:
LncRNA MEG3, a tumor suppressor gene, is related to reducing the proliferation, migration, and invasion
as well as apoptosis abilities of gastric cancer (GC), which is a promising therapeutic target in patients.
We conducted a comprehensive search of the literature on Pubmed using the keywords “lncRNA MEG3 and
gas cancer” from 2014 to the present. Here, we provide a systematic and comprehensive summary of existing
knowledge of the lncRNAs MEG3 and reveal its biological function and specific mechanisms in gastric cancer.
MEG3 is involved in many molecular mechanisms that inhibit the development and progression of gastric
cancer. For example, MEG3 can inhibit the proliferation of gastric cancer cells by inhibiting the expression of
miR-21, miR-665, miR-148, miR-208, etc. MEG3 inhibits gastric carcinogenesis by inhibiting the negative
regulator MDM2, regulating the expression of tumor suppressor genes p53 and Rb gene, and managing
PI3K/Akt and Wnt/β-catenin signaling pathways. Additionally, gastric cancer patients with low MEG3 expression
have poor prognosis, and transfection of MEG3 can improve the overall survival time of normal cells.
Eventually, lncRNA MEG3 can be used as a biomarker or target for intervention, thereby providing new insights
for gastric cancer therapy.