bluuebunny/crossref_metadata_2025_split huggingface dataset:
AL360181.1 promotes proliferation and invasion in colon cancer and is one of ten m6A-related lncRNAs that predict overall survival
Authors: Luo Yi , Xie Yayun , Wu Dejun , Wang Bingyi , Lu Helei , Wang Zhiqiang , Quan Yingjun , Han Bo 
Published: November 2023

Background
N6-methyladenosine (m6A) exerted a pivotal role in colon cancer. Nevertheless, the long non-coding RNAs (lncRNAs) associated with this process have yet to be elucidated.

Methods
The open-access data used for analysis was downloaded from The Cancer Genome Atlas (TCGA) database for analysis, employing the R software for computational evaluations. The RNA level of specific molecules was assessed using the quantitative real-time PCR. CCK8, colony formation and transwell assay were used to evaluate the proliferation, invasion and migration ability of colon cancer cells.

Results
Here, we identified the m6A regulators from TCGA data and subsequently pinpointed lncRNAs with a —Cor— > 0.3 and P < 0.05, categorizing them as m6A-associated lncRNAs. Moreover, we formulated a prognosis signature rooted in ten m6A-related lncRNAs, consisting of AL360181.1, PCAT6, SNHG26, AC016876.1, AC104667.2, AL114730.3, LINC02257, AC147067.1, AP006621.3 and AC009237.14. This signature exhibited notable predictive accuracy in gauging patient survival. Immune-related evaluations revealed varied immune cell infiltration patterns across different risk groups, with our findings suggesting superior immunotherapy response in low-risk patients. Biological enrichment analysis indicated that the high-risk patients had a higher activity of multiple carcinogenic pathways, including glycolysis. The previously unreported lncRNA, AL360181.1, displayed a connection to glycolytic activity and diminished survival rates, warranting further investigation. The result indicated that AL360181.1 was correlated with more aggressive clinical characteristics. Immune infiltration assessments found AL360181.1 to have a positive correlation with Tcm infiltration, but an inverse relationship with entities like Th2 cells, T cells, neutrophils and macrophages. Biological enrichment analysis indicated that the pathways of WNT/β-catenin, pancreas beta cells, hedgehog signaling and some metabolism pathways were upregulated in high AL360181.1 patients. In vitro experiments showed that AL360181.1 was upregulated in the colon cancer cells. Moreover, AL360181.1 significantly promotes the proliferation, invasion and migration of colon cancer cells.

Conclusions
Our results can provide direction for future studies on m6A-related lncRNA in colon cancer.
Elucidating the role of nicotinamide N-methyltransferase-p53 axis in the progression of chronic kidney disease
Authors: Zhen Xin , Sun Yuxiang , Lin Hongchun , Huang Yuebo , Liu Tianwei , Li Yuanqing , Peng Hui 
Published: November 2023

Background
Chronic kidney disease (CKD) is a significant global health issue characterized by progressive loss of kidney function. Renal interstitial fibrosis (TIF) is a common feature of CKD, but current treatments are seldom effective in reversing TIF. Nicotinamide N-methyltransferase (NNMT) has been found to increase in kidneys with TIF, but its role in renal fibrosis is unclear.

Methods
Using mice with unilateral ureteral obstruction (UUO) and cultured renal interstitial fibroblast cells (NRK-49F) stimulated with transforming growth factor-β1 (TGF-β1), we investigated the function of NNMT in vivo and in vitro.

Results
We performed single-cell transcriptome sequencing (scRNA-seq) on the kidneys of mice and found that NNMT increased mainly in fibroblasts of UUO mice compared to sham mice. Additionally, NNMT was positively correlated with the expression of renal fibrosis-related genes after UUO injury. Knocking down NNMT expression reduced fibroblast activation and was accompanied by an increase in DNA methylation of p53 and a decrease in its phosphorylation.

Conclusions
Our findings suggest that chronic kidney injury leads to an accumulation of NNMT, which might decrease p53 methylation, and increase the expression and activity of p53. We propose that NNMT promotes fibroblast activation and renal fibrosis, making NNMT a novel target for preventing and treating renal fibrosis.
Factors Influencing Choice of Place for Child Delivery among Child Bearing-Aged Women in Balambala Sub-County, Garissa County, Kenya
Authors: Mohamed Ibrahim Gedi , Odongo Alfred Owino , Mogere Dominic 
Published: November 2023

Background: There has been little progress made in Sub-Saharan Africa in achieving Sustainable Development Goal of reducing maternal deaths. Maternal mortality can be reduced significantly by skilled delivery. Women in low-income countries choose home deliveries despite the risks. In Kenya, maternity services are offered free-of-charge in all public health facilities. Mothers receive non-cash incentives after delivery. Investigated independent variables were health care related factors, gynecological factors and socio-cultural factors. The dependent variable was the proportions of hospital and, home deliveries. Maternal mortality rate in Balambala was found to be 587/100,000 while the national and county averages were 223 and 530/100,000 respectively. The study investigated factors influencing choice of place of child delivery.
Methods: Cross-sectional study was used and participants recruited through randomization. A sample size of 384 mothers, who had 1-year-old child, registered for ante-natal care services in latest pregnancy in Balambala were interviewed at the households. Quantitative data collected using structured questionnaires and analyzed using Statistical Package for Social Sciences version 25. P-value of .05 was set as statistical significance. Descriptive and inferential statistics were calculated, nature and magnitude of association between dependent and independent variables investigated.
Results: The independent variables recorded the following; p-value of 0.042 for socio-cultural factors, p-value of 0.039 for obstetric factors and p-value of 0.035 for health care related factors.
Conclusion: Proportions of home and hospital deliveries were 52% and 48% respectively. The independent variables were statistically significant, p-value<.05. The researcher recommends inclusion of literacy lessons into the present ANC profile. Husbands to accompany their pregnant wives to health facilities at least 2 of the 4 scheduled ANC visits.
Effect of Work Motivation on Job Performance among Healthcare Providers in Eradah and Mental Health Complex in Jeddah Region, Saudi Arabia
Authors: Altamimi Abdalrhman , Khodary Emad faroqu , Alawi Abdulrahman Thabet , Alawi Ahmed Thabet , Banjar Hatim , Alhindi Yaser Mohammad , Shabi Ismail Hadi 
Published: November 2023

Aims: This study aimed to assess work motivation levels among healthcare providers in the Eradah Complex and evaluate healthcare providers’ job performance, considering various dimensions.
Study Design: A cross sectional study design.
Place and Duration of Study: The target population for this research comprises healthcare providers working within the Eradah Complex in Jeddah, Saudi Arabia, including a diverse range of professionals, such as doctors, nurses, administrative staff, and other healthcare personnel, who collectively contribute to providing healthcare services within the complex, between June 2023 and September 2023.
Methodology: A cross-sectional design with comprising healthcare providers working in Eradah Complex in Jeddah, with diverse range of professionals, such as doctors, nurses, and administrative staff, Data for this study were collected through the administration of a structured questionnaire and then the data for this study was collected from healthcare providers working within the Eradah Complex in Jeddah, Saudi Arabia. Data from the structured questionnaires had been distributed within two-week timeframe for the participants to complete the questionnaires. Data reliability and statistical analyses using appropriate tests was done using SPSS version 25.0 program.
Results: This study involved 212 participants from different age groups with a mean age of 37.6±1.3 years old, and predominant male gender (75%), educational level, and professional status with majority (29.7%) of nurses participated in this study, that there was a significant difference in Work Motivation according to Profession (F= 2.68, P-value = 0.011), but there was no significant difference in the other demographic characteristics (age, gender, marital status, education level, and experience year, and there was a significant difference in Job Performance according to age (F= 3.655, P-value = 0.028).
Conclusion: It was observed that there was a significant effect of work motivation on job performance, so the work motivational process may enhance the job performance among healthcare workers in some of Jeddah hospitals.
External Validation and Updating of a Statistical Civilian-Based Suicide Risk Model in US Naval Primary Care
Authors: Ripperger Michael A. , Kolli Jhansi , Wilimitis Drew , Robinson Katelyn , Reale Carrie , Novak Laurie L. , Cunningham Craig A. , Kasuske Lalon M. , Grover Shawna G. , Ribeiro Jessica D. , Walsh Colin G. 
Published: November 2023

ImportanceSuicide remains an ongoing concern in the US military. Statistical models have not been broadly disseminated for US Navy service members.ObjectiveTo externally validate and update a statistical suicide risk model initially developed in a civilian setting with an emphasis on primary care.Design, Setting, and ParticipantsThis retrospective cohort study used data collected from 2007 through 2017 among active-duty US Navy service members. The external civilian model was applied to every visit at Naval Medical Center Portsmouth (NMCP), its NMCP Naval Branch Health Clinics (NBHCs), and TRICARE Prime Clinics (TPCs) that fall within the NMCP area. The model was retrained and recalibrated using visits to NBHCs and TPCs and updated using Department of Defense (DoD)–specific billing codes and demographic characteristics, including expanded race and ethnicity categories. Domain and temporal analyses were performed with bootstrap validation. Data analysis was performed from September 2020 to December 2022.ExposureVisit to US NMCP.Main Outcomes and MeasuresRecorded suicidal behavior on the day of or within 30 days of a visit. Performance was assessed using area under the receiver operating curve (AUROC), area under the precision recall curve (AUPRC), Brier score, and Spiegelhalter z-test statistic.ResultsOf the 260 583 service members, 6529 (2.5%) had a recorded suicidal behavior, 206 412 (79.2%) were male; 104 835 (40.2%) were aged 20 to 24 years; and 9458 (3.6%) were Asian, 56 715 (21.8%) were Black or African American, and 158 277 (60.7%) were White. Applying the civilian-trained model resulted in an AUROC of 0.77 (95% CI, 0.74-0.79) and an AUPRC of 0.004 (95% CI, 0.003-0.005) at NBHCs with poor calibration (Spiegelhalter P &amp;lt; .001). Retraining the algorithm improved AUROC to 0.92 (95% CI, 0.91-0.93) and AUPRC to 0.66 (95% CI, 0.63-0.68). Number needed to screen in the top risk tiers was 366 for the external model and 200 for the retrained model; the lower number indicates better performance. Domain validation showed AUROC of 0.90 (95% CI, 0.90-0.91) and AUPRC of 0.01 (95% CI, 0.01-0.01), and temporal validation showed AUROC of 0.75 (95% CI, 0.72-0.78) and AUPRC of 0.003 (95% CI, 0.003-0.005).Conclusions and RelevanceIn this cohort study of active-duty Navy service members, a civilian suicide attempt risk model was externally validated. Retraining and updating with DoD-specific variables improved performance. Domain and temporal validation results were similar to external validation, suggesting that implementing an external model in US Navy primary care clinics may bypass the need for costly internal development and expedite the automation of suicide prevention in these clinics.
Laser Epilation as an Adjunct to Standard Care in Reducing Pilonidal Disease Recurrence in Adolescents and Young Adults
Authors: Minneci Peter C. , Gil Lindsay A. , Cooper Jennifer N. , Asti Lindsey , Nishimura Leah , Lutz Carley M. , Deans Katherine J. 
Published: November 2023

ImportanceRecurrence continues to be a significant challenge in the treatment and management of pilonidal disease.ObjectiveTo compare the effectiveness of laser epilation (LE) as an adjunct to standard care vs standard care alone in preventing recurrence of pilonidal disease in adolescents and young adults.Design, Setting, and ParticipantsThis was a single-institution, randomized clinical trial with 1-year follow-up conducted from September 2017 to September 2022. Patients aged 11 to 21 years with pilonidal disease were recruited from a single tertiary children’s hospital.InterventionLE and standard care (improved hygiene and mechanical or chemical depilation) or standard care alone.Main Outcomes and MeasuresThe primary outcome was the rate of recurrence of pilonidal disease at 1 year. Secondary outcomes assessed during the 1-year follow-up included disability days, health-related quality of life (HRQOL), health care satisfaction, disease-related attitudes and perceived stigma, and rates of procedures, surgical excisions, and postoperative complications.ResultsA total of 302 participants (median [IQR] age, 17 [15-18] years; 157 male [56.1%]) with pilonidal disease were enrolled; 151 participants were randomly assigned to each intervention group. One-year follow-up was available for 96 patients (63.6%) in the LE group and 134 (88.7%) in the standard care group. The proportion of patients who experienced a recurrence within 1 year was significantly lower in the LE treatment arm than in the standard care arm (−23.2%; 95% CI, −33.2 to −13.1; P &amp;lt; .001). Over 1 year, there were no differences between groups in either patient or caregiver disability days, or patient- or caregiver-reported HRQOL, health care satisfaction, or perceived stigma at any time point. The LE group had significantly higher Child Attitude Toward Illness Scores (CATIS) at 6 months (median [IQR], 3.8 [3.4-4.2] vs 3.6 [3.2-4.1]; P = .01). There were no differences between groups in disease-related health care utilization, disease-related procedures, or postoperative complications.Conclusions and RelevanceLE as an adjunct to standard care significantly reduced 1-year recurrence rates of pilonidal disease compared with standard care alone. These results provide further evidence that LE is safe and well tolerated in patients with pilonidal disease. LE should be considered a standard treatment modality for patients with pilonidal disease and should be available as an initial treatment option or adjunct treatment modality for all eligible patients.Trial RegistrationClinicalTrials.gov Identifier: NCT03276065
Comparison of visual and semi‐automated kilovoltage cone beam CT image QA analysis
Authors: Becerra‐Espinosa Nicholas , Claps Lindsey , Alaei Parham 
Published: November 2023

AbstractEstablished kilovoltage cone‐beam computed tomography (kV‐CBCT) image quality assurance (QA) guidelines often rely on recommendations provided by the American Association of Physicists in Medicine (AAPM) task group (TG) reports with metrics that use visual analysis. This can result in measurement variations by different users, especially in visually subjective analyzes such as low contrast resolution. Consequently, there is a growing interest in more automated means of image QA analysis that can offer increased consistency, accuracy, and convenience. This work compares visual QA to semi‐automated software QA analysis to establish the performance and viability of a semi‐automated method.In this study, a commercial product (RIT Radia. Radiological Imaging Technology, Colorado Springs, CO) was used to evaluate 68 months of kV‐CBCT images of a Catphan® 504 phantom obtained from a Varian TrueBeam® linear accelerator. Six key metrics were examined: high contrast resolution, low contrast resolution, Hounsfield unit constancy, uniformity and noise, and spatial linearity. The results of this method were then compared to those recorded visually using Bland‐Altman, and/or paired sample t‐test.Comparison of all modules showed a non‐random, statistically significant difference between visual and semi‐automated methods except for LDPE and Teflon in the Hounsfield unit constancy analysis, which falls outside the paired sample t‐test's 5% significance level. A small high contrast resolution bias indicates the two analysis methods are largely equivalent, while a large low contrast resolution bias indicates greater semi‐automated target detection. Wide limits of agreement in the uniformity module suggests variability due to multiple visual observers. Spatial linearity results measured differences of less than 0.17%.Semi‐automated QA analysis offered greater stability over visual analysis. Additionally, semi‐automated QA results satisfied or exceeded visual QA passing criteria and allowed for fast and consistent image quality analysis.
Development and Evaluation of a Nomogram for Predicting the Outcome of Immune Reconstitution Among HIV/AIDS Patients Receiving Antiretroviral Therapy in China
Authors: Wang Yi , Liu Shourong , Zhang Wenhui , Zheng Liping , Li Er , Zhu Mingli , Yan Dingyan , Shi Jinchuan , Bao Jianfeng , Yu Jianhua 
Published: November 2023

AbstractThis study aims to develop and evaluate a model to predict the immune reconstitution among HIV/AIDS patients after antiretroviral therapy (ART). A total of 502 HIV/AIDS patients are randomized to the training cohort and evaluation cohort. Least absolute shrinkage and selection operator (LASSO) regression and multivariate logistic regression analysis are performed to identify the indicators and establish the nomogram for predicting the immune reconstitution. Decision curve analysis (DCA) and clinical impact curve (CIC) are used to evaluate the clinical effectiveness of the nomogram. Predictive factors included white blood cells (WBC), baseline CD4+ T‐cell counts (baseline CD4), ratio of effector regulatory T cells to resting regulatory T cells (eTreg/rTreg) and low‐density lipoprotein cholesterol (LDL‐C) and are incorporated into the nomogram. The area under the curve (AUC) is 0.812 (95% CI, 0.767∼0.851) and 0.794 (95%CI, 0.719∼0.857) in the training cohort and evaluation cohort, respectively. The calibration curve shows a high consistency between the predicted and actual observations. Moreover, DCA and CIC indicate that the nomogram has a superior net benefit in predicting poor immune reconstitution. A simple‐to‐use nomogram containing four routinely collected variables is developed and internally evaluated and can be used to predict the poor immune reconstitution in HIV/AIDS patients after ART.
Triboelectricity Based Self‐Powered Digital Displacement Sensor for Aircraft Flight Actuation
Authors: Zhou Zhuyu , Xu Zijie , Cao Leo N.Y. , Sheng Hengrui , Li Chengyu , Shang Yurui , Tang Wei , Wang Zhong Lin 
Published: November 2023

AbstractThe utilization of unmanned aerial vehicles (UAVs) is on the rise across various industries. In such a scenario, the issue of flight safety for these UAVs becomes increasingly paramount. Currently, UAVs exhibit shortcomings in flight attitude perception compared to more mature manned aircraft, especially concerning the position sensing of flight actuation, which poses significant safety risks. Mature position monitoring solutions for flight actuation used in manned aircraft cannot be directly integrated into systems of UAV due to compatibility issues. This necessitates the development of new position sensing technologies to address this challenge. Triboelectric nanogenerators, with their advantages of miniaturization, self‐powering capabilities, and the ability to generate voltage‐level electrical signals, are chosen to form a part of the position detection system for sensors in UAVs. In this study, a self‐powered displacement sensor is developed that utilizes frictional charge separation signals. This sensor is specifically designed to monitor the position status of the flight actuators in UAV. With a compact volume of <11.1 cm3 and a weight of <9.5 g, this sensor is lightweight efficient and adaptable for practical applications.
Thermal Rectification in Modularly Designed Bulk Metamaterials
Authors: He Hongying , Peng Weixiang , Le Ferrand Hortense 
Published: November 2023

AbstractThermal rectification is a phenomenon of great practical importance where heat transfer is preferential in one direction. Programmable control of heat transfer in 3D space is key to enable thermal rectification at the macroscale but is rarely realized in natural materials or in current existing devices that are constructed at the nano and microscales with high system complexity. Here, modularly designed bulk metamaterials that can break the symmetry of heat transfer from one direction to the other are created, leading to thermal rectification in convergent or divergent states by tuning the metamaterial microstructural design. These thermal metamaterials are microstructured composites made using one material composition, however, they offer sufficient microstructural design freedom to allow tunable local thermal properties for unusual macroscopic heat transfer. The strategy and performance achieved are promising for next‐generation thermal management.
Hydrothermal and Mechanosynthesis of Mixed‐Cation Double Perovskite Scintillators for Radiation Detection
Authors: O'Neill Joseph , Ghosh Joydip , Alghamdi Suad , Braddock Isabel , Crean Carol , Dorey Robert , Mulholland Roma , Richards Sion , Wilson Matthew , Salway Hayden , Anaya Miguel , Reiss Justin , Wolfe Douglas , Sellin Paul 
Published: November 2023

AbstractThis article details work performed on the synthesis and characterization of an inorganic mixed‐cation double halide perovskite, Cs2Ag.6Na.4In.85Bi.15Cl6 (CANIBIC). Single crystals have been created via a hydrothermal reaction, milled into a powder, and pressed into pellets, while nanocrystals have been directly synthesized via mechanosynthesis. A computational model is constructed to predict the X‐ray diffraction pattern of CANIBIC; this model aligns very well with the X‐ray diffraction pattern measured for CANIBIC crystal powder. This model can therefore be developed in the future as a tool to predict lattice parameters and crystal structures of other novel double‐halide perovskites. Photoluminescence spectra obtained from each format show broad emission centered at 630 nm, as is typical for self‐trapped exciton emission; self‐trapped exciton emission is also confirmed by investigating photoluminescence intensity as a function of laser power. Nanocomposites are produced via the loading of nanocrystals of CANIBIC into PMMA. Although nanocomposite disks consisting of a small proportion of CANIBIC nanocrystals in PMMA have a smaller mass attenuation coefficient than a pressed pellet of CANIBIC, these disks have comparatively bright radioluminescence due to their optical transparency. These nanocomposite disks are therefore a particularly useful format for the practical use of the CANIBIC scintillator.