bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of Willingness to Participate in COVID‐19 Screening Testing from a Pilot School Survey in the United States
Authors: Mark‐Carew Miguella , Swanson Megan , Eller Brian , Cullen Theresa , Valenzuela Manuel O. , LaBelle Monica , Persad Neela , Barrios Lisa C. , Szucs Leigh E. 
Published: October 2023

ABSTRACTBACKGROUNDCOVID‐19 screening testing (ST) can detect asymptomatic or pre‐symptomatic cases, allowing for prompt identification of cases and close contacts. This study examined parents' and school staffs' knowledge and attitudes toward to a pilot school‐based ST program in a school district in southern Arizona.METHODSIn May 2021, online surveys to parents and school staff were administered to examine attitudes toward ST and impacts of the COVID‐19 pandemic. Unweighted percent estimates were calculated, and bivariate differences were examined by demographics. Associations were assessed using chi‐square tests and logistic regression.RESULTSThe survey had response rates of 10% (606/6085) and 22% (187/849) among parents and staff, respectively. Approximately one‐third of responding parents (35%) would or already allow their child to participate in school‐based ST, 37% would not participate; 28% were unsure. Among responding staff, 46% would or already participate in ST, 33% would not; 21% were unsure. The top concern (38%) among responding staff was taking job‐related leave if testing positive.CONCLUSIONSchools work to balance the needs of students, families, and staff by implementing supportive and flexible policies and practices founded on buy‐in and acceptance from their communities
A cytosystematic study of the Dianthus virgineus complex (Caryophyllaceae) in the Central Mediterranean
Authors: Franzoni Jacopo , Astuti Giovanni , Bacchetta Gianluigi , Barone Giulio , Bartolucci Fabrizio , Bernardo Liliana , Carta Angelino , Conti Fabio , Domina Gianniantonio , Frajman Božo , Giusso del Galdo Gianpietro , Iamonico Duilio , Iberite Mauro , Minuto Luigi , Sarigu Marco , Terlević Ana , Turini Alessia , Varaldo Lucia , Volgger Daniel , Peruzzi Lorenzo 
Published: October 2023

AbstractEuropean wild carnations (Dianthus) are represented by a high number of taxa organized in unresolved taxonomies. In particular, taxa belonging to the Dianthus virgineus L. complex in the Central Mediterranean have been delimited mainly with qualitative morphological data and still await quantitative investigations, which are vital to understand boundaries and relations among plant diversity groups. Here, we examine the phenotypic features of nuclear genome organization testing for species boundaries in this complex. We have studied the chromosome number, the total haploid length (THL), and the relative genome size (RGS) in 122 populations belonging to 25 out of 33 taxa of the complex. All the studied populations have 2n = 2x = 30 chromosomes, and the THL ranges from 14.09 to 20.71 μm. Genome size estimations support the absence of polyploidization events, but show a certain degree of variation (0.318–0.423 arbitrary units). The RGS variation is not in agreement with current taxonomic treatment, but rather shows a geographical pattern, with higher values in Sicily and Sardinia. No correlation between the THL and the RGS was detected, possibly due to the stable chromosome number and the small size of chromosomes. A number of evolutionary unique groups lower than the number of currently accepted taxa may be hypothesized.
The MIO1‐MtKIX8 module regulates the organ size in Medicago truncatula
Authors: Mao Yawen , Zhou Shaoli , Yang Jing , Wen Jiangqi , Wang Dongfa , Zhou Xuan , Wu Xinyuan , He Liangliang , Liu Mingli , Wu Huan , Yang Liling , Zhao Baolin , Tadege Million , Liu Yu , Liu Changning , Chen Jianghua 
Published: October 2023

AbstractPlant organ size is an important agronomic trait tightly related to crop yield. However, the molecular mechanisms underlying organ size regulation remain largely unexplored in legumes. We previously characterized a key regulator F‐box protein MINI ORGAN1 (MIO1)/SMALL LEAF AND BUSHY1 (SLB1), which controls plant organ size in the model legume Medicago truncatula. In order to further dissect the molecular mechanism, MIO1 was used as the bait to screen its interacting proteins from a yeast library. Subsequently, a KIX protein, designated MtKIX8, was identified from the candidate list. The interaction between MIO1 and MtKIX8 was confirmed further by Y2H, BiFC, split‐luciferase complementation and pull‐down assays. Phylogenetic analyses indicated that MtKIX8 is highly homologous to Arabidopsis KIX8, which negatively regulates organ size. Moreover, loss‐of‐function of MtKIX8 led to enlarged leaves and seeds, while ectopic expression of MtKIX8 in Arabidopsis resulted in decreased cotyledon area and seed weight. Quantitative reverse‐transcription PCR and in situ hybridization showed that MtKIX8 is expressed in most developing organs. We also found that MtKIX8 serves as a crucial molecular adaptor, facilitating interactions with BIG SEEDS1 (BS1) and MtTOPLESS (MtTPL) proteins in M. truncatula. Overall, our results suggest that the MIO1‐MtKIX8 module plays a significant and conserved role in the regulation of plant organ size. This module could be a good target for molecular breeding in legume crops and forages.
Spatial, seasonal and climatic drivers of suspended sediment atop Great Bahama Bank
Authors: Lopez‐Gamundi Cecilia , Barnes Brian B. , Bakker Anna C. , Harris Paul (Mitch) , Eberli Gregor P. , Purkis Sam J. 
Published: October 2023

ABSTRACTSuspension is the key mechanism by which fine‐grained sediment (≤125 μm) is winnowed and transported across shallow‐water carbonate platforms into adjacent deep waters. Unlike sliding and saltation, which deliver sedimentary structures via bedload, the sedimentological signature of suspended sediment is more cryptic. This study focuses on suspended sediment, and its drivers – wind, waves and tides – to better constrain the processes responsible for the transport of fine‐grained sediments. By building forward from remote sensing algorithms developed for deep‐waters, sediment suspension in the shallow water column can be mapped from satellite. By applying machine learning to Moderate Resolution Imaging Spectroradiometer data for Great Bahama Bank, this study demonstrates how the drivers of sediment suspension change over 18 years across this 100 000 km2 carbonate platform. Through time, both seasonal patterns of suspension, as well as those induced by El Niño‐Southern Oscillation and, more subtly, the Atlantic Meridional Overturning Circulation were tracked. El Niño‐Southern Oscillation modulates wind‐induced currents, while Atlantic Meridional Overturning Circulation affects local sea level. Across space, this study shows how the eastern margin of Great Bahama Bank, which is traditionally considered to be wind‐dominated, primarily owes its suspended sediment to tidal currents focused between islands. Sediment suspension across the leeward margin of Great Bahama Bank, meanwhile, can be attributed to wind‐induced currents and waves. The authors consider this work a step towards delivering a quantitative, reproducible, process‐based understanding of sediment suspension atop carbonate platforms using Earth observation data.
Efficacy of a multimodal approach of laser therapy for earlobe keloids management in dark population
Authors: Piccolo Domenico , Crisman Giuliana , Conforti Claudio , Fusco Irene , Bonan Paolo 
Published: October 2023

AbstractBackgroundPatients with keloids are often younger than 30 years old and have darker skin.AimTo evaluate the efficacy and safety of CO2 laser therapy in the management of earlobe and helix keloids in dark subjects.MethodsA total of 21 dark patients (five men and 16 women) presented with the earlobe and ear helix keloids, with a mean age of 30.5 (±6.7) years and with phototype between III–VI, were enrolled. In order to improve their earlobe and helix keloids, all patients underwent a single session of CO2 laser therapy immediately followed by a pulsed Dye laser procedure. A single well‐experienced doctor performed all treatment sessions and the response to treatment. Patients were followed‐up for 6 months after termination of therapy. Clinical images were examined and collected: all patients were examined clinically and with Dermoscopy.ResultsThe majority of the patients treated show complete remission of keloid lesions. Clinical photographs show a visible aesthetic improvement of several types of keloids in dark subjects.ConclusionsOur data demonstrate that this combined laser therapeutic protocol appears to be effective and well tolerated for the management of earlobe and helix keloids in dark subjects, with no high recurrence rate and avoiding the adverse effects and lengthy recovery time.
Solutions of the Diophantine EquationsBr=Js+JtandCr=Js+Jt
Authors: Gaber Ahmed , Ahmed Mohiedeen 
Published: October 2023

LetBrr≥0,Jrr≥0, andCrr≥0be the balancing, Jacobsthal, and Lucas balancing numbers, respectively. In this paper, the diophantine equationsBr=Js+JtandCr=Js+Jtare completely solved. The solutions rely basically on Matveev’s theorem on linear forms in logarithms of algebraic numbers and a procedure of reducing the upper bound due to Dujella and Pethö.
Infusion Product TNFα, Th2, and STAT3 Activities Are Associated with Clinical Responses to Transgenic T-cell Receptor Cell Therapy
Authors: Nowicki Theodore S. , Peters Cole W. , Quiros Crystal , Kidd Conner K. , Kawakami Moe , Klomhaus Alexandra M. , Baselga-Carretero Ignacio , Kaplan-Lefko Paula , Macabali Mignonette H. , Perez Garcilazo Ivan , Berent-Maoz Beata , Comin-Anduix Begoña , Ribas Antoni 
Published: October 2023

Abstract
Transgenic T-cell receptor (TCR) T cell–based adoptive cell therapies for solid tumors are associated with dramatic initial response rates, but there remain many instances of treatment failure and disease relapse. The association of infusion product cytokine profiles with clinical response has not been explored in the context of TCR T-cell therapy products. Single-cell antigen-dependent secretomic and proteomic analysis of preinfusion clinical TCR T-cell therapy products revealed that TNFα cytokine functionality of CD8+ T cells and phospho-STAT3 signaling in these cells were both associated with superior clinical responsiveness to therapy. By contrast, CD4+ T-helper 2 cell cytokine profiles were associated with inferior clinical responses. In parallel, preinfusion levels of IL15, Flt3-L, and CX3CL1 were all found to be associated with clinical response to therapy. These results have implications for the development of therapeutic biomarkers and identify potential targets for enrichment in the design of transgenic TCR T-cell therapies for solid tumors.
Combining magnetoencephalography with telemetric streaming of intracranial recordings and deep brain stimulation—A feasibility study
Authors: Hnazaee Mansoureh Fahimi , Sure Matthias , O’Neill George C. , Leogrande Gaetano , Schnitzler Alfons , Florin Esther , Litvak Vladimir 
Published: October 2023

Abstract
The combination of subcortical Local Field Potential (LFP) recordings and stimulation with Magnetoencephalography (MEG) in Deep Brain Stimulation (DBS) patients enables the investigation of cortico-subcortical communication patterns and provides insights into DBS mechanisms. Until now, these recordings have been carried out in post-surgical patients with externalised leads. However, a new generation of telemetric stimulators makes it possible to record and stream LFP data in chronically implanted patients. Nevertheless, whether such streaming can be combined with MEG has not been tested. In the present study, we tested the most commonly implanted telemetric stimulator—Medtronic Percept PC with a phantom in three different MEG systems: two cryogenic scanners (CTF and MEGIN) and an experimental Optically Pumped Magnetometry (OPM)-based system. We found that when used in combination with the new SenSight segmented leads, Percept PC telemetric streaming only generates band-limited interference in the MEG at 123 Hz and harmonics. However, the “legacy streaming mode” used with older lead models generates multiple, dense artefact peaks in the physiological range of interest (below 50 Hz). The effect of stimulation on MEG critically depends on whether it is done in bipolar (between two contacts on the lead) or monopolar (between a lead contact and the stimulator case) mode. Monopolar DBS creates severe interference in the MEG as previously reported. However, we found that the OPM system is more resilient to this interference and could provide artefact-free measurements, at least for limited frequency ranges. A resting measurement in the MEGIN system from a Parkinson’s patient implanted with Percept PC and subthalamic SenSight leads revealed artefact patterns consistent with our phantom recordings. Moreover, analysis of LFP-MEG coherence in this patient showed oscillatory coherent networks consistent in their frequency and topography with those described in published group studies done with externalised leads. In conclusion, Percept PC telemetric streaming with SenSight leads is compatible with MEG. Furthermore, OPM sensors could provide additional new opportunities for studying DBS effects.
Dynamically Downscaled Projections of Phenological Changes across the Contiguous United States
Authors: Mallard Megan S. , Talgo Kevin D. , Spero Tanya L. , Bowden Jared H. , Nolte Christopher G. 
Published: October 2023

Abstract
Phenological indicators (PI) are used to study changes to animal and plant behavior in response to seasonal cycles, and they can be useful to quantify the potential impacts of climate change on ecosystems. Here, multiple global climate models and emission scenarios are used to drive dynamically downscaled simulations using the WRF Model over the contiguous United States (CONUS). The wintertime dormancy of plants [chilling units (CU)], timing of spring onset [extended spring indices (SI)], and frequency of proceeding false springs are calculated from regional climate simulations covering historical (1995–2005) and future periods (2025–2100). Southern parts of the CONUS show projected CU decreases (inhibiting some plants from flowering or fruiting), while the northern CONUS experiences an increase (possibly causing plants to break dormancy too early, becoming vulnerable to disease or freezing). Spring advancement (earlier SI dates) is projected, with decadal trends ranging from approximately 1–4 days per decade over the CONUS, comparable to or exceeding those found in observational studies. Projected changes in risk of false spring (hard freezes following spring onset) vary across members of the ensemble and regions of the CONUS, but generally western parts of the CONUS are projected to experience increased risk of false springs. These projected changes to PI connote significant effects on cycles of plants, animals, and ecosystems, highlighting the importance of examining temperature changes during transitional seasons.

Significance Statement
This study examines how phenological indicators, which track the life cycles of plants and animals, could change from 2025 to 2100 as simulated in a regional climate model over the contiguous United States. Chilling units quantify the presence of cooler weather that can benefit plants prior to their growing season. They are projected to decrease in the southern United States, possibly inhibiting agricultural production. Spring onset is projected to occur earlier in the year, advancing by 1–4 days on average over each future decade. Risk of false springs (damaging hard freezes after spring onset) increases in the western United States. Our findings highlight the need to understand effects of climate change during transitional seasons, which can impact agriculture and ecosystems.
Use of ceftriaxone as an alternative treatment method in pregnant women diagnosed with syphilis – a single centre experience
Authors: Hartmane Ilona , Ivdra Iveta , Mikazans Ingmars , Princevs Aleksejs , Teterina Irena , Bondare-Ansberga Vanda , Reinberga Lelde 
Published: October 2023

Background There are few data on the use of ceftriaxone in pregnant women diagnosed with syphilis. The aim of this study was to investigate the safety and efficacy of ceftriaxone as an alternative treatment option for syphilis during pregnancy. Methods A retrospective analysis of 79 pregnant women diagnosed with syphilis and treated with ceftriaxone was conducted. Results No cases of intolerance, Jarisch-Herxheimer reactions, or allergic reactions were recorded. The average time to seronegativation for secondary syphilis with symptoms was 6.14 months ± 2.76, and for latent forms, it was 7.52 months ± 1.84. Patients received no additional treatment. No serious adverse drug reactions were reported. Conclusions Data from our study support the use of ceftriaxone as an effective and safe alternative treatment for pregnant women diagnosed with syphilis when penicillin therapy is contraindicated or unavailable.
Pranic Healing as a Complementary Therapy in Diabetic Foot Ulcer Management: A Randomised, Controlled, Double-Blind Trial
Authors: Nittur Anuradha , Pavan Belehalli , Ganapathy Raghavan , Dorai Vinod Kumar , Singhal Shivam 
Published: October 2023

Background Diabetic Foot Ulcers (DFUs), a serious complication of diabetes, have limited solutions in conventional therapies. The condition needs holistic management of blood glycemic levels; foot wounds; and possible regeneration of nerves in the soles of patients. Objective To evaluate the efficacy of Pranic Healing as a complimentary therapy in managing Diabetic Foot Ulcers (DFUs). Methods Thirty diabetic subjects already on standard therapy for Diabetes, co morbidities and wound care for Diabetic Foot Ulcers were assigned on a 1:1 basis to the trial and control groups. While both groups continued to receive standard therapy, the trial group additionally received Pranic Healing therapy. The Clinician, nursing staff, assessors and patients were blinded. Pranic Healers carried out healing on the trial group remotely, every day for 50 to 60 minute for stress, diabetes, local wound healing, blood cleansing and regeneration of nerves in the soles. The efficacy variables were the mean change from baseline in wound parameters and grade of DFU, overall well-being and HbAlc levels. Results At the end of the trial, about 83.33% participants in the trial group demonstrated lowering of size and severity of the ulceration and improved to a lower grade of DFU compared to 44.4% in control group. The trial group reported a significant reduction in wound area and HbA1c levels. The trial group showed better readings for improved sensory perception in the soles through changes in the large fibre dysfunction and damaged nerves as compared to the control group. Approximately 76.9% of participants in the trial group reported lower stress levels compared to 22.22% in the control group. Conclusions Pranic Healing intervention can be a safe and effective adjunct in managing Diabetic Foot Ulcers.
Gata2 noncoding genetic variation as a determinant of hematopoietic stem/progenitor cell mobilization efficiency
Authors: Soukup Alexandra A. , Bresnick Emery H. 
Published: October 2023

Abstract
Germline genetic variants alter the coding and enhancer sequences of GATA2, which encodes a master regulator of hematopoiesis. The conserved murine Gata2 enhancer (+9.5) promotes hematopoietic stem cell (HSC) genesis during embryogenesis. Heterozygosity for a single-nucleotide Ets motif variant in the human enhancer creates a bone marrow failure and acute myeloid leukemia predisposition termed GATA2 deficiency syndrome. The homozygous murine variant attenuates chemotherapy- and transplantation-induced hematopoietic regeneration, hematopoietic stem and progenitor cell (HSPC) response to inflammation, and HSPC mobilization with the therapeutic mobilizer granulocyte colony–stimulating factor (G-CSF). Because a Gata2 +9.5 variant attenuated G-CSF–induced HSPC expansion and mobilization, and HSC transplantation therapies require efficacious mobilization, we tested whether variation affects mechanistically distinct mobilizers or only those operating through select pathways. In addition to affecting G-CSF activity, Gata2 variation compromised IL-8/CXCR2- and VLA-4/VCAM1-induced mobilization. Although the variation did not disrupt HSPC mobilization mediated by plerixafor, which functions through CXCR4/CXCL12, homozygous and heterozygous variation attenuated mobilization efficacy of the clinically used plerixafor/G-CSF combination. The influence of noncoding variation on HSPC mobilization efficacy and function is important clinically because comprehensive noncoding variation is not commonly analyzed in patients. Furthermore, our mobilization-defective system offers unique utility for elucidating fundamental HSPC mechanisms.
Improved phyllosphere microbiome composition of tea plant with the application of small peptides in combination with rhamnolipid
Authors: Chen Hao , Song Yujie , Wang Shuangshuang , Fan Kai , Wang Hui , Mao Yilin , Zhang Jie , Xu Yang , Yin Xinyue , Wang Yu , Ding Zhaotang 
Published: October 2023

Abstract
Background
Small peptides play a crucial role in plant growth and adaptation to the environment. Exogenous small peptides are often applied together with surfactants as foliar fertilizers, but the impact of small peptides and surfactants on the tea phyllosphere microbiome remains unknown.

Results
In this study, we investigated the effects of small peptides and different surfactants on the tea phyllosphere microbiome using 16S and ITS sequencing. Our results showed that the use of small peptides reduced the bacterial diversity of the tea phyllosphere microbiome and increased the fungal diversity, while the use of surfactants influenced the diversity of bacteria and fungi. Furthermore, the addition of rhamnolipid to small peptides significantly improved the tea phyllosphere microbiome community structure, making beneficial microorganisms such as Pseudomonas, Chryseobacterium, Meyerozyma, and Vishniacozyma dominant populations.

Conclusion
Our study suggests that the combined use of small peptides and surfactants can significantly modify the tea phyllosphere microbiome community structure, particularly for beneficial microorganisms closely related to tea plant health. Thus, this preliminary study offers initial insights that could guide the application of small peptides and surfactants in agricultural production, particularly with respect to their potential for modulating the phyllosphere microbiome community in tea plant management.