bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gesundheit und Klimawandel – welche Potenziale haben versorgungsnahe Daten?
Authors: Günster Christian , Schmuker Caroline 
Published: January 2024

ZusammenfassungDieser Beitrag geht der Frage nach, welche Auswirkungen der Klimawandel auf die Gesundheit haben kann und inwieweit versorgungsnahe Daten zur Forschung in diesem Themenfeld beitragen können. Der Klimawandel verändert die Umwelt- und Lebensbedingungen der Menschheit, er ist damit auch zu einem relevanten Gesundheitsproblem geworden. Die Zunahme von Extremwetterereignissen, Veränderungen bei der UV- und Luftschadstoffbelastung sowie die klimaassoziierte Verbreitung von Allergenen oder neuartigen Erregern verändern das Spektrum an Erkrankungen und den medizinischen Versorgungsbedarf in der Bevölkerung erheblich. Allerdings liegen bislang nur wenige Erkenntnisse zu den Folgen für das Gesundheitssystem und zu besonders betroffenen Bevölkerungsgruppen in Deutschland vor. Versorgungsnahe Daten (Primärdaten, Registerdaten, Sekundärdaten) in Verbindung mit Umweltexpositionsdaten und modulierenden Daten (z. B. sozioökonomische Daten) haben das Potenzial, die Forschung zu den gesundheitlichen Folgen des Klimawandels erheblich voranzubringen. Dieser Beitrag benennt die Veränderungen der Umwelt- und Lebensbedingungen sowie der damit verbundenen gesundheitlichen Risiken. Er beschreibt die Datengrundlagen, die grundsätzlich zur Analyse gesundheitlicher Auswirkungen des Klimawandels zur Verfügung stehen. An einem konkreten Beispiel wird aufgezeigt, wie die Zusammenführung von individuellen Gesundheitsdaten (hier GKV-Abrechnungsdaten), Umweltexpositionsdaten und modulierenden Daten gelingen kann. Der Beitrag bietet abschließend eine umfassende Übersicht über offene Forschungsfragen, die mit versorgungsnahen Daten beantwortet werden können.
Unklare Minderung des Allgemeinzustandes bei Patientin mit Lungenkarzinom
Authors: Brägelmann J. , Becker S. , Stenzel D. , Budahn A. 
Published: January 2024

ZusammenfassungEine 63-jährige Patientin mit Lungenkarzinom stellte sich erstmalig in unserer Notaufnahme mit plötzlicher Reduktion des Allgemeinzustands, Erbrechen und ausgeprägter Schwäche vor. Sie gab an, aufgrund des Lungenkarzinoms eine Chemotherapie zu erhalten, und verneinte weitere, relevante Vorerkrankungen. Unsere initiale Verdachtsdiagnose waren eine zytostatikainduzierte Nausea und Emesis. Die in der Notaufnahme durchgeführte Diagnostik erbrachte entgegen dieser Verdachtsdiagnose die Befunde einer Ketoazidose auf dem Boden einer Erstmanifestation eines Diabetes mellitus mit hyperglykämischer Entgleisung sowie einer schweren, manifesten Hypothyreose. Nach Beschaffung der Vorbefunde wurde evident, dass die Patientin keine Chemotherapie, sondern eine Immuncheckpointtherapie mittels Durvalumab erhielt. Die beschriebenen Erstmanifestationen waren demzufolge als durvalumabassoziierte Immunreaktionen zu werten. Nach Einleitung einer diabetischen Rekompensationstherapie und Substitution mittels L‑Thyroxin konnte eine rasche Verbesserung des Allgemeinzustands erreicht werden. Wären für uns relevante Vorbefunde schon zu Behandlungsbeginn abrufbar gewesen, beispielsweise durch die elektronische Patientenakte, wären die korrekte Interpretation der Symptome und die korrekte Therapie früher möglich gewesen.
Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat
Authors: Lin Min , Islamov Bulat , Aleliūnas Andrius , Armonienė Rita , Gorash Andrii , Meigas Egon , Ingver Anne , Tamm Ilmar , Kollist Hannes , Strazdiņa Vija , Bleidere Māra , Brazauskas Gintaras , Lillemo Morten 
Published: January 2024

Abstract
Key message
QPm.NOBAL-3A is an important QTL providing robust adult plant powdery mildew resistance in Nordic and Baltic spring wheat, aiding sustainable crop protection and breeding.

Abstract
Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici, poses a significant threat to bread wheat (Triticum aestivum L.), one of the world’s most crucial cereal crops. Enhancing cultivar resistance against this devastating disease requires a comprehensive understanding of the genetic basis of powdery mildew resistance. In this study, we performed a genome-wide association study (GWAS) using extensive field trial data from multiple environments across Estonia, Latvia, Lithuania, and Norway. The study involved a diverse panel of recent wheat cultivars and breeding lines sourced from the Baltic region and Norway. We identified a major quantitative trait locus (QTL) on chromosome 3A, designated as QPm.NOBAL-3A, which consistently conferred high resistance to powdery mildew across various environments and countries. Furthermore, the consistency of the QTL haplotype effect was validated using an independent Norwegian spring wheat panel. Subsequent greenhouse seedling inoculations with 15 representative powdery mildew isolates on a subset of the GWAS panel indicated that this QTL provides adult plant resistance and is likely of race non-specific nature. Moreover, we developed and validated KASP markers for QPm.NOBAL-3A tailored for use in breeding. These findings provide a critical foundation for marker-assisted selection in breeding programs aimed at pyramiding resistance QTL/genes to achieve durable and broad-spectrum resistance against powdery mildew.
Solving quasimonotone and non-monotone variational inequalities
Authors: Uzor V. A. , Alakoya T. O. , Mewomo O. T. , Gibali A. 
Published: January 2024

AbstractWe present a simple iterative method for solving quasimonotone as well as classical variational inequalities without monotonicity. Strong convergence analysis is given under mild conditions and thus generalize the few existing results that only present weak convergence methods under restrictive assumptions. We give finite and infinite dimensional numerical examples to compare and illustrate the simplicity and computational advantages of the proposed scheme.
Molecular mechanism of anticancer effect of heat shock protein 90 inhibitor BIIB021 in human bladder cancer cell line
Authors: Asdemir Aydemir , Özgür Aykut 
Published: January 2024

AbstractBladder cancer is a type of urologic malignancy that exhibits significant morbidity, mortality, and treatment costs. Inhibition of heat shock protein 90 (HSP90) activity has been a promising pharmacological strategy for blocking of bladder cancer pathogenesis. BIIB021 is a next-generation HSP90 inhibitor which interrupts ATP hydrolysis process of HSP90 and inhibits the stabilization and correct folding of client proteins. In current study, we aimed to investigate the molecular mechanism of the anticancer activity of BIIB021 in human bladder cancer T24 cells. Our results revealed that nanomolar concentration of BIIB021 decreased viability of T24 cell. BIIB021 downregulated HSP90 expression in T24 cells and inhibited the refolding activity of luciferase in the presence of T24 cell lysate. PCR array data indicated a significant alteration in transcript levels of cancer-related genes involved in metastases, apoptotic cell death, cell cycle, cellular senescence, DNA damage and repair mechanisms, epithelial-to-mesenchymal transition, hypoxia, telomeres and telomerase, and cancer metabolism pathways in T24 cells. All findings hypothesize that BIIB021 could exhibit as effective HSP90 inhibitor in the future for treatment of bladder cancer patients.
Multiplexed electrochemical liposomes applied to the detection of nucleic acids for Influenza A, Influenza B and SARS-CoV-2
Authors: Gerstl Florian , Loessl Michael , Borggraefe Veronika , Baeumner Antje J. 
Published: January 2024

AbstractMultiplexing is a relevant strategy for biosensors to improve accuracy and decision-making due to the increased amount of simultaneously obtained information. Liposomes offer unique benefits for label-based multiplexing since a variety of different marker molecules can be encapsulated, leading to intrinsic signal amplification and enabling a variety of detection formats. We successfully developed an electrochemical (EC) liposome-based platform technology for the simultaneous detection of at least three analytes by studying parameters to ensure specific and sensitive bioassay performance. Influenza A and B and SARS-CoV-2 sequences served as model system in a standard sandwich hybridization assay. Studies included encapsulants, probe distribution on liposomes and capture beads, assay setup and interferences between liposomes to also ensure a generalization of the platform. Ruthenium hexamine(III), potassium hexacyanoferrate(II) and m-carboxy luminol, when encapsulated separately into a liposome, provided desirable long-term stability of at least 12 months and no cross-signals between liposomes. Through the optimization process, low limits of detections of 1.6 nmol L−1, 125 pmol L−1 and 130 pmol L−1, respectively, were achieved in a multiplexed assay setup, which were similar to singleplex assays. Non-specific interactions were limited to 25.1%, 7.6% and 7.5%, respectively, through sequential liposome incubations and singleplex capture bead designs. Here, ruthenium hexamine liposomes had only mediocre performance so that low overall signal strength translated into higher LODs and worse specificity. A different marker such as ferroin may be an option in the future. The identification of further electrochemical markers will provide new opportunities for liposomes to function as multiplex, orthogonal or internal standard labels in electrochemical bioassays.
Graphical Abstract
Dissipation in Onsager’s Critical Classes and Energy Conservation in $$BV\cap L^\infty $$ with and Without Boundary
Authors: De Rosa Luigi , Inversi Marco 
Published: January 2024

AbstractThis paper is concerned with the incompressible Euler equations. In Onsager’s critical classes we provide explicit formulas for the Duchon–Robert measure in terms of the regularization kernel and a family of vector-valued measures$$\{\mu _z\}_z\subset {\mathcal {M}}_{x,t}$${μz}z⊂Mx,t, having some Hölder regularity with respect to the direction$$z\in B_1$$z∈B1. Then, we prove energy conservation for$$L^\infty _{x,t}\cap L^1_t BV_x$$Lx,t∞∩Lt1BVxsolutions, in both the absence or presence of a physical boundary. This result generalises the previously known case of Vortex Sheets, showing that energy conservation follows from the structure of$$L^\infty \cap BV$$L∞∩BVincompressible vector fields rather than the flow having “organized singularities”. The interior energy conservation features the use of Ambrosio’s anisotropic optimization of the convolution kernel and it differs from the usual energy conservation arguments by heavily relying on the incompressibility of the vector field. This is the first energy conservation proof, for a given class of solutions, which fails to simultaneously apply to both compressible and incompressible models, coherently with compressible shocks having non-trivial entropy production. To run the boundary analysis we introduce a notion of “normal Lebesgue trace” for general vector fields, very reminiscent of the one forBVfunctions. We show that having such a null normal trace is basically equivalent to have vanishing boundary energy flux. This goes beyond the previous approaches, laying down a setup which applies to every Lipschitz bounded domain. Allowing any Lipschitz boundary introduces several technicalities to the proof, with a quite geometrical/measure-theoretical flavour.
Is Osteogenesis Imperfecta Associated with Cardiovascular Abnormalities? A Systematic Review of the Literature
Authors: Verdonk Sara J. E. , Storoni Silvia , Micha Dimitra , van den Aardweg Joost G. , Versacci Paolo , Celli Luca , de Vries Ralph , Zhytnik Lidiia , Kamp Otto , Bugiani Marianna , Eekhoff Elisabeth M. W. 
Published: January 2024

AbstractOsteogenesis imperfecta (OI) is a rare genetic disorder caused by abnormal collagen type I production. While OI is primarily characterized by bone fragility and deformities, patients also have extraskeletal manifestations, including an increased risk of cardiovascular disease. This review provides a comprehensive overview of the literature on cardiovascular diseases in OI patients in order to raise awareness of this understudied clinical aspect of OI and support clinical guidelines. In accordance with the PRISMA guidelines, a systematic literature search in PubMed, Embase, Web of Science and Scopus was conducted that included articles from the inception of these databases to April 2023. Valvular disease, heart failure, atrial fibrillation, and hypertension appear to be more prevalent in OI than in control individuals. Moreover, a larger aortic root was observed in OI compared to controls. Various cardiovascular diseases appear to be more prevalent in OI than in controls. These cardiovascular abnormalities are observed in all types of OI and at all ages, including young children. As there are insufficient longitudinal studies, it is unknown whether these abnormalities are progressive in nature in OI patients. Based on these findings, we would recommend referring individuals with OI to a cardiologist with a low-threshold.
Towards validation in clinical routine: a comparative analysis of visual MTA ratings versus the automated ratio between inferior lateral ventricle and hippocampal volumes in Alzheimer’s disease diagnosis
Authors: Wittens Mandy M. J. , Allemeersch Gert-Jan , Sima Diana M. , Vanderhasselt Tim , Raeymaeckers Steven , Fransen Erik , Smeets Dirk , de Mey Johan , Bjerke Maria , Engelborghs Sebastiaan 
Published: January 2024

Abstract
Purpose
To assess the performance of the inferior lateral ventricle (ILV) to hippocampal (Hip) volume ratio on brain MRI, for Alzheimer’s disease (AD) diagnostics, comparing it to individual automated ILV and hippocampal volumes, and visual medial temporal lobe atrophy (MTA) consensus ratings.

Methods
One-hundred-twelve subjects (mean age ± SD, 66.85 ± 13.64 years) with varying degrees of cognitive decline underwent MRI using a Philips Ingenia 3T. The MTA scale by Scheltens, rated on coronal 3D T1-weighted images, was determined by three experienced radiologists, blinded to diagnosis and sex. Automated volumetry was computed by icobrain dm (v. 5.10) for total, left, right hippocampal, and ILV volumes. The ILV/Hip ratio, defined as the percentage ratio between ILV and hippocampal volumes, was calculated and compared against a normative reference population (n = 1903). Inter-rater agreement, association, classification accuracy, and clinical interpretability on patient level were reported.

Results
Visual MTA scores showed excellent inter-rater agreement. Ordinal logistic regression and correlation analyses demonstrated robust associations between automated brain segmentations and visual MTA ratings, with the ILV/Hip ratio consistently outperforming individual hippocampal and ILV volumes. Pairwise classification accuracy showed good performance without statistically significant differences between the ILV/Hip ratio and visual MTA across disease stages, indicating potential interchangeability. Comparison to the normative population and clinical interpretability assessments showed commensurability in classifying MTA “severity” between visual MTA and ILV/Hip ratio measurements.

Conclusion
The ILV/Hip ratio shows the highest correlation to visual MTA, in comparison to automated individual ILV and hippocampal volumes, offering standardized measures for diagnostic support in different stages of cognitive decline.
Establishing a High-Quality Pediatric Cardiac Surgery Program in Post-Conflict Regions: A Model for Limited Resource Countries
Authors: Youssef Tammam , Bitar Fouad , Alogla Hassanain , El Khoury Maya , Moukhaiber Jihan , Alamin Farah , AlHareth Bassam , Gabriel Cristoveanu Catalin , Youssef Rana , Abouzahr Labib , Abdul Sater Zahi , Bitar Fadi 
Published: January 2024

Abstract

Background
Congenital Heart Disease stands as a prominent cause of infant mortality, with notable disparities in surgical outcomes evident between high-income and low- to middle-income countries.

Objective
This study presents a collaborative partnership between a local governmental entity and an international private organization to establish a high-quality Pediatric Cardiac Surgery Program in a post-conflict limited resource country, Iraq.

Methods
A descriptive retrospective study analyzed pediatric cardiac surgery procedures performed by a visiting pediatric heart surgery team from October 2021 to October 2022, funded by the Ministry of Health (MOH). We used the STS-EACTS complexity scoring model (STAT) to assess mortality risks associated with surgical procedures.

Results
A total of 144 patients underwent 148 procedures. Infants comprised 58.3% of the patients. The most common anomalies included tetralogy of Fallot, ventricular septal defect, and various single ventricle categories, constituting 76% of the patient cohort. The overall surgical mortality rate was 4.1%, with an observed/expected surgical mortality rate of 1.1 (95% CI 0.5, 2.3). There was no significant difference between our observed surgical mortality in Category 2, 3, and 4 and those expected/reported by the STS-EACTS Database (p = 0.07, p = 0.72, and p = 0.12, respectively). The expenses incurred by the MOH for conducting surgeries in Iraq were lower than the alternative of sending patients abroad for the same procedures.

Conclusion
The partnership model between a local public entity committed to infrastructure development and funding and an international private organization delivering clinical and training services can provide the foundation for building sustainable, high-quality in situ programs in upper-middle-income countries.
Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production
Authors: Lu Ping , Bai Ruoxuan , Gao Ting , Chen Jiale , Jiang Ke , Zhu Yalun , Lu Ye , Zhang Shuting , Xu Fangxu , Zhao Hongxin 
Published: January 2024

Abstract
2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in Enterobacter aerogenes. Firstly, the pyruvate metabolic network was reconstructed by deleting genes for by-product synthesis to improve the flux toward 2,3-BDO synthesis, which resulted in a 90% increase of the product titer. Secondly, the 2,3-BDO productivity of the IAM1183-LPCT/D was increased by 55% due to the heterologous expression of DR1558 which boosted cell resistance to abiotic stress. Thirdly, carbon sources were optimized to further improve the yield of target products. The IAM1183-LPCT/D showed the highest titer of 2,3-BDO from sucrose, 20% higher than that from glucose, and the yield of 2,3-BDO reached 0.49 g/g. Finally, the titer of 2,3-BDO of IAM1183-LPCT/D in a 5-L fermenter reached 22.93 g/L, 85% higher than the wild-type strain, and the titer of by-products except ethanol was very low.

Key points

Deletion of five key genes in E. aerogenes improved 2,3-BDO production

The titer of 2,3-BDO was increased by 90% by regulating metabolic flux

Response regulator DR1558 was expressed to increase 2,3-BDO productivity

Graphical abstract
Nitrogen balance and supply in Australasian mushroom composts
Authors: Noble Ralph , Thai Meghann , Kertesz Michael A. 
Published: January 2024

Abstract
Mushrooms are an important source of protein in the human diet. They are increasingly viewed as a sustainable meat replacement in an era of growing populations, with button mushrooms (Agaricus bisporus) the most popular and economically important mushroom in Europe, Australia and North America. Button mushrooms are cultivated on a defined, straw-derived compost, and the nitrogen (N) required to grow these high-protein foods is provided mainly by the addition of poultry manure and horse manure. Using the correct balance of carbon (C) and N sources to produce mushroom compost is critically important in achieving maximum mushroom yields. Changes in the amount and form of N added, the rate and timing of N addition and the other compost components used can dramatically change the proportion of added N recovered in the mushroom caps, the yield and quality of the mushrooms and the loss of N as ammonia and nitrogen oxide gases during composting. This review examines how N supply for mushroom production can be optimised by the use of a broad range of inorganic and organic N sources for mushroom composting, together with the use of recycled compost leachate, gypsum and protein-rich supplements. Integrating this knowledge into our current molecular understanding of mushroom compost biology will provide a pathway for the development of sustainable solutions in mushroom production that will contribute strongly to the circular economy.

Key points
• Nitrogen for production of mushroom compost can be provided as a much wider range of organic feedstocks or inorganic compounds than currently used
• Most of the nitrogen used in production of mushroom compost is not recovered as protein in the mushroom crop
• The sustainability of mushroom cropping would be increased through alternative nitrogen management during composting and cropping
New biomarkers underlying acetic acid tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii
Authors: Samakkarn Wiwan , Vandecruys Paul , Moreno Maria Remedios Foulquié , Thevelein Johan , Ratanakhanokchai Khanok , Soontorngun Nitnipa 
Published: January 2024

Abstract
Evolutionary engineering experiments, in combination with omics technologies, revealed genetic markers underpinning the molecular mechanisms behind acetic acid stress tolerance in the probiotic yeast Saccharomyces cerevisiae var. boulardii. Here, compared to the ancestral Ent strain, evolved yeast strains could quickly adapt to high acetic acid levels (7 g/L) and displayed a shorter lag phase of growth. Bioinformatic-aided whole-genome sequencing identified genetic changes associated with enhanced strain robustness to acetic acid: a duplicated sequence in the essential endocytotic PAN1 gene, mutations in a cell wall mannoprotein (dan4Thr192del), a lipid and fatty acid transcription factor (oaf1Ser57Pro) and a thiamine biosynthetic enzyme (thi13Thr332Ala). Induction of PAN1 and its associated endocytic complex SLA1 and END3 genes was observed following acetic acid treatment in the evolved-resistant strain when compared to the ancestral strain. Genome-wide transcriptomic analysis of the evolved Ent acid-resistant strain (Ent ev16) also revealed a dramatic rewiring of gene expression among genes associated with cellular transport, metabolism, oxidative stress response, biosynthesis/organization of the cell wall, and cell membrane. Some evolved strains also displayed better growth at high acetic acid concentrations and exhibited adaptive metabolic profiles with altered levels of secreted ethanol (4.0–6.4% decrease), glycerol (31.4–78.5% increase), and acetic acid (53.0–60.3% increase) when compared to the ancestral strain. Overall, duplication/mutations and transcriptional alterations are key mechanisms driving improved acetic acid tolerance in probiotic strains. We successfully used adaptive evolutionary engineering to rapidly and effectively elucidate the molecular mechanisms behind important industrial traits to obtain robust probiotic yeast strains for myriad biotechnological applications.

Key points
•Acetic acid adaptation of evolutionary engineered robust probiotic yeast S. boulardii
•Enterol ev16 with altered genetic and transcriptomic profiles survives in up to 7 g/L acetic acid
•Improved acetic acid tolerance of S. boulardii ev16 with mutated PAN1, DAN4, OAF1, and THI13 genes

Graphical Abstract