bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA adduct formation by the environmental contaminant 3‐nitrobenzanthrone after intratracheal instillation in rats
Authors: Bieler Christian A. , Cornelius Michael G. , Klein Reinhold , Arlt Volker M. , Wiessler Manfred , Phillips David H. , Schmeiser Heinz H. 
Published: April 2005

Abstract3‐Nitrobenzanthrone (3‐NBA) is an environmental pollutant and suspected human carcinogen found in emissions from diesel and gasoline engines and on the surface of ambient air particulate matter; human exposure to 3‐NBA is likely to occur primarily via the respiratory tract. In our study female Sprague Dawley rats were treated by intratracheal instillation with a single dose of 0.2 or 2 mg/kg body weight of 3‐NBA. Using the butanol enrichment version of the 32P‐postlabeling method, DNA adduct formation by 3‐NBA 48 hr after intratracheal administration in different organs (lung, pancreas, kidney, urinary bladder, heart, small intestine and liver) and in blood was investigated. The same adduct pattern consisting of up to 5 DNA adduct spots was detected by thin layer chromatography in all tissues and blood and at both doses. Highest total adduct levels were found in lung and pancreas (350 ± 139 and 620 ± 370 adducts per 108 nucleotides for the high dose and 39 ± 18 and 55 ± 34 adducts per 108 nucleotides for the low dose, respectively) followed by kidney, urinary bladder, heart, small intestine and liver. Adduct levels were dose‐dependent in all organs (approximately 10‐fold difference between doses). It was demonstrated by high performance liquid chromatography (HPLC) that all 5 3‐NBA‐derived DNA adducts formed in rats after intratracheal instillation are identical to those formed by other routes of application and are, as previously shown, formed from reductive metabolites bound to purine bases. Although total adduct levels in the blood were much lower (41 ± 27 and 9.5 ± 1.9 adducts per 108 nucleotides for the high and low dose, respectively) than those found in the lung, they were related to dose and to the levels found in lung. These results show that uptake of 3‐NBA by the lung induces high levels of specific DNA adducts in several organs of the rat and an identical adduct pattern in DNA from blood. Therefore, 3‐NBA‐DNA adducts present in the blood are useful biomarkers for exposure to 3‐NBA and may help to assess the effective biological dose in humans exposed to it. © 2005 Wiley‐Liss, Inc.
Vaccination with dendritic cells pulsed with apoptotic tumors in combination with anti‐OX40 and anti‐4‐1BB monoclonal antibodies induces T cell–mediated protective immunity in Her‐2/neu transgenic mice
Authors: Cuadros Camilo , Dominguez Ana Lucia , Lollini Pier‐Luigi , Croft Michael , Mittler Robert S. , Borgström Per , Lustgarten Joseph 
Published: April 2005

AbstractTumor cells express tumor‐associated antigens (TAAs), which can serve as targets for the immune system. However, the majority of TAAs are overexpressed products of normal cellular genes; as such, self‐tolerance mechanisms have hindered their use for the induction of effective antitumor responses. One such normal self‐protein is the growth factor receptor Her‐2/neu, which is overexpressed in 25–35% of all mammary carcinomas in humans. In previous studies, we have demonstrated that Her‐2/neu mice are functionally tolerant to neu antigens and contain only a low avidity T‐cell repertoire to neu antigens. However, this residual low‐avidity T‐cell repertoire has antitumor activity. In this study, we compared the immune responses of Her‐2/neu mice immunized with dendritic cells (DCs) pulsed with soluble neu protein or with apoptotic tumor cells. Analysis of the antitumor response shows that Her‐2/neu mice vaccinated with DCs pulsed with Her‐2/neu antigens retard tumor growth; however, vaccination with DCs pulsed with apoptotic tumor cells induces a stronger antitumor effect. Administration of multiple immunizations in combination with the costimulatory agonist anti‐OX40 or anti‐4‐1BB MAb significantly enhanced the immune responses in these mice, resulting in complete tumor rejection if the tumor burden was small and substantial tumor reduction with a larger tumor burden. These results have important implications for the design of tumor vaccination strategies, suggesting that the use of vaccines that stimulate a broad immune response in combination with costimulatory molecules as immunomodulators could significantly improve the antitumor immune response in tolerant hosts. © 2005 Wiley‐Liss, Inc.
Suppression of the TIG3 tumor suppressor gene in human ovarian carcinomas is mediated via mitogen‐activated kinase‐dependent and ‐independent mechanisms
Authors: Lotz Kristina , Kellner Tobias , Heitmann Michaela , Nazarenko Irina , Noske Aurelia , Malek Anastassia , Gontarewicz Artur , Schäfer Reinhold , Sers Christine 
Published: April 2005

AbstractThe TIG3 gene is a retinoic acid inducible class II tumor suppressor gene downregulated in several human tumors and malignant cell lines. Diminished TIG3 expression correlates with decreased differentiation whereas forced expression of TIG3 suppresses oncogenic signaling pathways and subsequently induces differentiation or apoptosis in tumor cells. Analysis of TIG3 mRNA expression in a large set of cDNA pools derived from matched tumor and normal human tissues showed a significant downregulation of TIG3 in 29% of the cDNA samples obtained from ovarian carcinomas. Using in situ hybridization, we demonstrated expression of TIG3 in the epithelial lining of 7 normal ovaries but loss of TIG3 expression in 15/19 of human ovarian carcinoma tissues. In SKOV‐3, CAOV‐3 and ES‐2 ovarian carcinoma cell lines, downregulation of TIG3 mRNA was reversible and dependent on an activated MEK‐ERK signaling pathway. Re‐expression of TIG3 mRNA in these cells upon specific interference with the MEK‐pathway was correlated with growth inhibition of the cells. In OVCAR‐3 and A27/80 ovarian carcinoma cells, TIG3 suppression is MEK‐ERK independent, but expression could be reconstituted upon interferon gamma (IFNγ) induction. Overexpression of TIG3 in A27/80 ovarian carcinoma cells significantly impaired cell growth and despite increased mRNA levels, TIG3 protein was hardly detectable. These results suggest that TIG3 is negatively regulated by an activated MEK‐ERK signaling pathway. Further mechanisms must interfere with TIG3 expression that are independent of MEK and partially include interferon‐responsive components. © 2005 Wiley‐Liss, Inc.
Successful combination of local CpG‐ODN and radiotherapy in malignant glioma
Authors: Meng Yuxia , Carpentier Antoine F. , Chen Lin , Boisserie Gilbert , Simon Jean‐Marc , Mazeron Jean‐Jacques , Delattre Jean‐Yves 
Published: April 2005

AbstractOligodeoxynucleotides containing CpG motifs (CpG‐ODN) display broad immunostimulating activity and are currently under clinical trial in various malignancies, including recurrent glioblastomas. Combining CpG‐ODN with another therapy that could induce antigen release might enhance tumor‐specific immune response. We investigated whether radiotherapy (RT) could be associated advantageously to intratumoral injections of CpG‐ODN. Fisher rats bearing 9L glioma were treated with various combinations of RT and CpG‐28, an oligonucleotide with good immunostimulating activity. RT and CpG‐28 induced complete tumor remission in one‐third of the animals. When both treatments were combined, complete tumor remission was achieved in two‐thirds of the animals (p < 0.001 when compared to non‐treated rats, p < 0.03 when compared to CpG‐28 alone). Such efficacy was not observed in nude mice, underlying the role of T cells in antitumor effects. The combination of both treatments appeared optimal when the delay between RT and CpG‐28 administration was <3 days (from 100% survival for a 3 days delay, to 57% survival for a 21 days delay, p < 0.05). Tumor infiltration by immune cells and expression within tumors of the CpG receptor, TLR9, were not modified by irradiation. These results support an attractive strategy of sequential radiotherapy and immunotherapy by CpG‐ODN and have potential implications for future clinical trials with CpG‐ODN. © 2005 Wiley‐Liss, Inc.
Prognostic role of cyclooxygenase‐2 in neoadjuvant‐treated patients with squamous cell carcinoma of the esophagus
Authors: Sivula Anna , Buskens Christianne J. , van Rees Bastiaan P. , Haglund Caj , Offerhaus G. Johan A. , van Lanschot J. Jan B. , Ristimäki Ari 
Published: April 2005

AbstractBased on our previous demonstration that elevated cyclooxygenase‐2 (COX‐2) expression is a prognostic factor for reduced survival in patients with adenocarcinoma of the esophagus, the aim of our study was to analyze the role of COX‐2 expression in esophageal squamous cell carcinoma. We analyzed COX‐2 protein expression from 117 consecutive patients by immunohistochemistry using a COX‐2 specific monoclonal antibody. Eighty‐one patients had not received any therapy before surgery whereas 36 patients received neoadjuvant chemotherapy as part of a randomized controlled trial. In the patients who received no chemotherapy, COX‐2 expression was low in 75% and high in 25% of the specimens. In this patient group, high COX‐2 expression associated with distal location of the tumor (p = 0.02), but did not correlate with any other clinicopathological parameter tested, including overall survival. In the patient group who received neoadjuvant chemotherapy, postoperative COX‐2 expression was low in 69% and high in 31%. Interestingly, in this patient group low COX‐2 expression correlated with development of distant metastases (p = 0.03) and to reduced overall survival (p = 0.02). Our results show that the prognostic significance of COX‐2 depends on the histological type of esophageal carcinoma and preoperative treatment of the patient. In conclusion, COX‐2 is not a prognostic marker in squamous cell carcinoma of the esophagus, but low COX‐2 expression is associated with poor prognosis in the neoadjuvant‐treated patients. © 2005 Wiley‐Liss, Inc.
Energy decay for the wave equation with boundary and localized dissipations in exterior domains
Authors: Bae Jeong Ja , Nakao Mitsuhiro 
Published: April 2005

AbstractWe study a decay property of solutions for the wave equation with a localized dissipation and a boundary dissipation in an exterior domain Ω with the boundary ∂Ω = Γ0 ∪ Γ1, Γ0 ∩ Γ1 = ∅︁. We impose the homogeneous Dirichlet condition on Γ0 and a dissipative Neumann condition on Γ1. Further, we assume that a localized dissipation a(x)ut is effective near infinity and in a neighborhood of a certain part of the boundary Γ0. Under these assumptions we derive an energy decay like E(t) ≤ C(1 + t)–1 and some related estimates. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Shift operators contained in contractions and pseudocontinuable matrixvalued Schur functions
Authors: Boiko S. S. , Dubovoy V. K. , Fritzsche Bernd , Kirstein Bernd 
Published: April 2005

AbstractWe show that the pseudocontinuability of a matrix‐valued Schur function θ in the unit disk is completely determined by the properties of the maximal shift and maximal coshift contained in a corresponding contractive operator T which has the characteristic operator function θ.
A gaussian space of test functions
Authors: Jorgenson J. , Lang S. 
Published: April 2005

AbstractElsewhere we develop an aspect of analysis on the simplest space of type G/K when G = SL2(C) and K is the unitary subgroup, having to do with the analogue of Poisson's inversion formula and resulting additive zeta functions obtained by applying the Gauss transform. The present paper carries out systematically a number of foundational properties of gaussians on that space, which serve as test functions, give rise to explicit formulas, and lead immediately into the heat kernel, which is a gaussian. We prove approximation properties and the fact that the space of gaussians is dense in essentially anything space one wants. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Seiberg–Witten–Floer homology of a surface times a circle for non‐torsion spinℂ structures
Authors: Muñoz Vicente , Wang Bai‐Ling 
Published: April 2005

AbstractWe determine the Seiberg–Witten–Floer homology groups of the 3‐manifold Σ × 𝕊1, where Σ is a surface of genus g ≥ 2, together with its ring structure, for a Spinℂ structure with non‐vanishing first Chern class. We give applications to computing Seiberg–Witten invariants of 4‐manifolds which are connected sums along surfaces and also we reprove the higher type adjunction inequalities obtained by Oszváth and Szabó. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Characterization of left‐definiteness of Sturm–Liouville problems
Authors: Wei Guangsheng , Wu Jianhua 
Published: April 2005

AbstractThe left‐definiteness for both regular and singular Sturm–Liouville problems is characterized. We give a necessary and sufficient condition on the coefficients, the endpoints and the boundary conditions of Sturm–Liouville problems to determine the left‐definiteness. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Space‐time regularity of catalytic super‐Brownian motion
Authors: Zähle Henryk 
Published: April 2005

AbstractWe study the question for which catalysts does the catalytic super‐Brownian motion in R have a jointly continuous space‐time Lebesgue density. As it turns out, there is a large class of non‐atomic catalysts inducing a regular density. The latter can be characterized as the unique solution to a certain stochastic partial differential equation driven by an inhomogeneous space‐time white noise. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
On the global well‐posedness of discrete Boltzmann systems with chemical reaction
Authors: Oliveira Filipe , Soares A. J. 
Published: April 2005

AbstractWe study an initial‐boundary value problem in one‐space dimension for the discrete Boltzmann equation extended to a diatomic gas undergoing both elastic multiple collisions and chemical reactions. By integration of conservation equations, we prove a global existence result in the half‐space for small initial data N0∈ℬ︁∩L1. Copyright © 2005 John Wiley & Sons, Ltd.
Assessment of infant cry: Acoustic cry analysis and parental perception
Authors: LaGasse Linda L. , Neal A. Rebecca , Lester Barry M. 
Published: April 2005

AbstractInfant crying signals distress to potential caretakers who can alleviate the aversive conditions that gave rise to the cry. The cry signal results from coordination among several brain regions that control respiration and vocal cord vibration from which the cry sounds are produced. Previous work has shown a relationship between acoustic characteristics of the cry and diagnoses related to neurological damage, SIDS, prematurity, medical conditions, and substance exposure during pregnancy. Thus, assessment of infant cry provides a window into the neurological and medical status of the infant. Assessment of infant cry is brief and noninvasive and requires recording equipment and a standardized stimulus to elicit a pain cry. The typical protocol involves 30 seconds of crying from a single application of the stimulus. The recorded cry is submitted to an automated computer analysis system that digitizes the cry and either presents a digital spectrogram of the cry or calculates measures of cry characteristics. The most common interpretation of cry measures is based on deviations from typical cry characteristics. Another approach evaluates the pattern across cry characteristics suggesting erarousal or underarousal or difficult temperament. Infants with abnormal cries should be referred for a full neurological evaluation. The second function of crying—to elicit caretaking—involves parent perception of the infant's needs. Typically, parents are sensitive to deviations in cry characteristics, but their perception can be altered by factors in themselves (e.g., depression) or in the context (e.g., culture). The potential for cry assessment is largely untapped. Infant crying and parental response is the first language of the new dyadic relationship. Deviations in the signal and/or misunderstanding the message can compromise infant care, parental effectiveness, and undermine the budding relationship. © 2005 Wiley‐Liss, Inc. MRDD Research Reviews 2005;11:83–93.
Integral equation solution of heat extraction‐induced thermal stress in enhanced geothermal reservoirs
Authors: Ghassemi A. , Tarasovs S. , Cheng A. H.‐D. 
Published: April 2005

AbstractDuring fluid injection in enhanced geothermal systems, thermo‐mechanical processes can play an important role. In fact, the phenomena of reservoir seismicity and the variation of injectivity with respect to injection water temperature can be attributed to the induced thermal stresses. In this paper, a three‐dimensional integral equation formulation is presented for calculating thermally induced stresses associated with the cooling of a fracture in a geothermal reservoir. By utilizing Green's function in the integral equation, the three‐dimensional heat flow and stresses in the reservoir are modelled without discretizing the reservoir. The formulation is implemented in a computer program for the solution of injection into an infinite fracture as well as for the injection/extraction in an arbitrarily shaped fracture. Copyright © 2005 John Wiley & Sons, Ltd.
Preparation of poly(propylene‐co‐γ‐butyrolactone carbonate) and release profiles of drug‐loaded microcapsules
Authors: Lu Lingbin , Huang Kelong 
Published: April 2005

AbstractA new aliphatic poly(propylene‐co‐γ‐butyrolactone carbonate) (PPCG) was successfully synthesized through the copolymerization of carbon dioxide, propylene oxide (PO), and γ‐butyrolactone (GBL). GBL was inserted into the backbone of PO–CO2. The glass transition of PPCG was as high as 16 °C, far higher than that (−1.5 °C) of poly(propylene carbonate) (PPC). The decomposition temperatures of PPCG and PPC were only slightly different. Because of the existence of the GBL ester unit, PPCG had stronger degradability than PPC in a pH 7.4 phosphate‐buffered solution. However, when the PO/GBL ratio increased beyond 5:2, the excessive amount of GBL was not added to the polymerization. PPCG and PPC microcapsules were prepared by the water‐in‐oil‐in‐water multiple‐emulsion method. Glucose was encapsulated. The PPCG microcapsules, about 2 μm in diameter, had smooth and spherical surfaces. The glucose release test revealed that the glucose release speed of the PPCG–glucose microcapsules was more than eight times faster than that of the PPC–glucose microcapsules in a pH 7.4 phosphate‐buffered solution. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 2468–2475, 2005
Circular dichroism induced by the helical conformations of acylated chitosan derivatives bearing cinnamate chromophores
Authors: Wu Yusong , Seo Toshihiro , Maeda Shiro , Sasaki Takashi , Irie Satoshi , Sakurai Kensuke 
Published: April 2005

AbstractThrough regioselective modifications, some acylated chitosan derivatives,O‐cinnamoyl chitosans with degree of substitution (DS) varying from 0.8 to 2.0 andN‐fatty acyl‐O‐dicinnamoyl chitosans with different fatty acyl chain lengths (C2–C12), were prepared, and their chiroptical properties in dilute solutions were investigated by circular dichroism (CD). Exciton coupling between two vicinal cinnamoyl chromophores appended to the helical mainchains gave rise to bisignate Cotton effects (CEs), which were used to deduce the absolute sense of the twisting structures in solution phase. It was found that the absolute helicities vary with DS, length of the fatty chain, and solvent property, but are nearly independent of thermal stimulus. The molecular interactions (hydrogen bonding and hydrophobic interaction) involved possibly in the self‐assembled ordered structures were discussed. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 1354–1364, 2005
Structure alignment via Delaunay tetrahedralization
Authors: Roach Jeffrey , Sharma Shantanu , Kapustina Maryna , Carter Charles W. 
Published: April 2005

AbstractA novel protein structure alignment technique has been developed reducing much of the secondary and tertiary structure to a sequential representation greatly accelerating many structural computations, including alignment. Constructed from incidence relations in the Delaunay tetrahedralization, alignments of the sequential representation describe structural similarities that cannot be expressed with rigid‐body superposition and complement existing techniques minimizing root‐mean‐squared distance through superposition. Restricting to the largest substructure superimposable by a single rigid‐body transformation determines an alignment suitable for root‐mean‐squared distance comparisons and visualization. Restricted alignments of a test set of histones and histone‐like proteins determined superpositions nearly identical to those produced by the established structure alignment routines of DaliLite and ProSup. Alignment of three, increasingly complex proteins: ferredoxin, cytidine deaminase, and carbamoyl phosphate synthetase, to themselves, demonstrated previously identified regions of self‐similarity. All‐against‐all similarity index comparisons performed on a test set of 45 class I and class II aminoacyl‐tRNA synthetases closely reproduced the results of established distance matrix methods while requiring 1/16 the time. Principal component analysis of pairwise tetrahedral decomposition similarity of 2300 molecular dynamics snapshots of tryptophanyl‐tRNA synthetase revealed discrete microstates within the trajectory consistent with experimental results. The method produces results with sufficient efficiency for large‐scale multiple structure alignment and is well suited to genomic and evolutionary investigations where no geometric model of similarity is known a priori. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Characterization of oxygenated derivatives of isoprene related to 2‐methyltetrols in Amazonian aerosols using trimethylsilylation and gas chromatography/ion trap mass spectrometry
Authors: Wang Wu , Kourtchev Ivan , Graham Bim , Cafmeyer Jan , Maenhaut Willy , Claeys Magda 
Published: April 2005

AbstractIn the present study, we have tentatively identified the structures of three oxygenated derivatives of isoprene in Amazonian rain forest aerosols as the C5 alkene triols, 2‐methyl‐1,3,4‐trihydroxy‐1‐butene (cis and trans) and 3‐methyl‐2,3,4‐trihydroxy‐1‐butene. The formation of these oxygenated derivatives of isoprene can be explained by acid‐catalyzed ring opening of epoxydiol derivatives of isoprene, namely, 1,2‐epoxy‐2‐methyl‐3,4‐dihydroxybutane and 1,2‐dihydroxy‐2‐methyl‐3,4‐epoxybutane. The structural proposals of the C5 alkene triols were based on chemical derivatization reactions and detailed interpretation of electron and chemical ionization mass spectral data, including data obtained from first‐order mass spectra, deuterium labeling of the trimethylsilyl methyl groups, and MS2 ion trap experiments. The characterization of 2‐methyl‐1,3,4‐trihydroxy‐1‐butene (cis and trans) and 3‐methyl‐2,3,4‐trihydroxy‐1‐butene in forest aerosols is important from an atmospheric chemistry viewpoint in that these compounds hint at the formation of intermediate isomeric epoxydiol derivatives of isoprene and as such provide mechanistic insights into the formation of the previously reported 2‐methyltetrols through photooxidation of isoprene. Copyright © 2005 John Wiley & Sons, Ltd.