bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Bottom Friction in Mediating the Response of the Weddell Gyre Circulation to Changes in Surface Stress and Buoyancy Fluxes
Authors: Neme Julia , England Matthew H. , Hogg Andrew McC. , Khatri Hemant , Griffies Stephen M. 
Published: December 2023

Abstract
The Weddell Gyre is one of the dominant features of the Southern Ocean circulation and its dynamics have been linked to processes of climatic relevance. Variability in the strength of the gyre’s horizontal transport has been linked to heat transport toward the Antarctic margins and changes in the properties and rates of export of bottom waters from the Weddell Sea region to the abyssal global ocean. However, the precise physical mechanisms that force variability in the Weddell’s lateral circulation across different time scales remain unknown. In this study, we use a barotropic vorticity budget from a mesoscale eddy active model simulation to attribute changes in gyre strength to variability in possible driving processes. We find that the Weddell Gyre’s circulation is sensitive to bottom friction associated with the overflowing dense waters at its western boundary. In particular, an increase in the production of dense waters at the southwestern continental shelf strengthens the bottom flow at the gyre’s western boundary, yet this drives a weakening of the depth-integrated barotropic circulation via increased bottom friction. Strengthening surface winds initially accelerate the gyre, but within a few years the response reverses once dense water production and export increases. These results reveal that the gyre can weaken in response to stronger surface winds, putting into question the traditional assumption of a direct relationship between surface stress and gyre strength in regions where overflowing dense water forms part of the depth-integrated flow.
Using Radiosonde Observations to Assess the “Three Ingredients Method” to Forecast QLCS Mesovortices
Authors: Ungar Max D. , Coniglio Michael C. 
Published: December 2023

Abstract
A technique used widely to forecast the potential for QLCS mesovortices is known as the “Three Ingredients Method” (3IM). The 3IM states that mesovortices are favored where 1) the QLCS cold pool and ambient low-level shear are said to be nearly balanced or slightly shear dominant, 2) where the component of the 0–3-km wind shear normal to the convective line is ≥30 kt (1 kt ≈ 0.51 m s−1), and 3) where a rear-inflow jet or enhanced outflow causes a surge or bow along the convective line. Despite its widespread use in operational settings, this method has received little evaluation in formal literature. To evaluate the 3IM, radiosonde observations are compared to radar-observed QLCS properties. The distance between the gust front and high reflectivity in the leading convective line (the “U-to-R distance”), the presence of rear-inflow surges, and mesovortices (MVs) were each assessed across 1820 line segments within 50 observed QLCSs. Although 0–3-km line-normal wind shear is statistically different between MV-genesis and null segments, values are ≤30 kt for 44% of MV-genesis segments. The 0–6-km line-normal wind shear also shows strong discrimination between MV-genesis and null segments and displays the best linear relationship of the U-to-R distance (a measure of system balance) among layers tested, although the scatter and overlap in distributions suggest that many factors can impact MV genesis (as expected). Overall, most MVs occur where the U-to-R distance lies between −5 and 5 km in the presence of a rear-inflow surge, along with positive 0–1-km wind shear, 0–3-km wind shear > 10 kt, and 0–6-km wind shear > 20 kt (all line-normal).

Significance Statement
Near the leading edge of thunderstorm lines, areas of rotation that can produce tornadoes and strong winds (“mesovortices”) often develop rapidly. Despite advances in understanding mesovortices, few operational guidelines exist to anticipate their genesis. One popular method used to forecast mesovortices—the “Three Ingredients Method”—is evaluated in this study. Our work confirms the importance of two of the ingredients—a surge of outflow winds and thunderstorms that stay nearly atop the leading edge of the outflow. However, we find that many mesovortices occur below the threshold of low-level wind shear ascribed by the forecast method. Refinements to the method are suggested, including the favorable distance between the leading edge of the outflow and thunderstorm updrafts and lower bounds of wind shear over multiple layers, below which mesovortices may be unlikely.
The use of eye-tracking technology as a tool to evaluate social cognition in people with an intellectual disability: a systematic review and meta-analysis
Authors: Jenner L. A. , Farran E. K. , Welham A. , Jones C. , Moss J. 
Published: December 2023

Abstract
Background
Relatively little is known about social cognition in people with intellectual disability (ID), and how this may support understanding of co-occurring autism. A limitation of previous research is that traditional social-cognitive tasks place a demand on domain-general cognition and language abilities. These tasks are not suitable for people with ID and lack the sensitivity to detect subtle social-cognitive processes. In autism research, eye-tracking technology has offered an effective method of evaluating social cognition—indicating associations between visual social attention and autism characteristics. The present systematic review synthesised research which has used eye-tracking technology to study social cognition in ID. A meta-analysis was used to explore whether visual attention on socially salient regions (SSRs) of stimuli during these tasks correlated with degree of autism characteristics presented on clinical assessment tools.

Method
Searches were conducted using four databases, research mailing lists, and citation tracking. Following in-depth screening and exclusion of studies with low methodological quality, 49 articles were included in the review. A correlational meta-analysis was run on Pearson’s r values obtained from twelve studies, reporting the relationship between visual attention on SSRs and autism characteristics.

Results and conclusions
Eye-tracking technology was used to measure different social-cognitive abilities across a range of syndromic and non-syndromic ID groups. Restricted scan paths and eye-region avoidance appeared to impact people’s ability to make explicit inferences about mental states and social cues. Readiness to attend to social stimuli also varied depending on social content and degree of familiarity. A meta-analysis using a random effects model revealed a significant negative correlation (r = −.28, [95% CI −.47, −.08]) between visual attention on SSRs and autism characteristics across ID groups. Together, these findings highlight how eye-tracking can be used as an accessible tool to measure more subtle social-cognitive processes, which appear to reflect variability in observable behaviour. Further research is needed to be able to explore additional covariates (e.g. ID severity, ADHD, anxiety) which may be related to visual attention on SSRs, to different degrees within syndromic and non-syndromic ID groups, in order to determine the specificity of the association with autism characteristics.
Klebsiella pneumoniae in the intestines of Musca domestica larvae can assist the host in antagonizing the poisoning of the heavy metal copper
Authors: Yin Yansong , Wang Shumin , Zhang Kexin , Li Ying , Liu WenJuan , Zhang Qian , Zhang Xinyu , Kong Xinxin , An Sha , Zhang Ruiling , Zhang Zhong 
Published: December 2023

AbstractBackgroundMusca domesticalarvae are common saprophytes in nature, promoting the material—energy cycle in the environment. However, heavy metal pollution in the environment negatively affects their function in material circulation. Our previous research found that some intestinal bacteria play an important role in the development of housefly, but the responses of microbial community to heavy metal stresses inMusca domesticais less studied.ResultsIn this study, CuSO4, CuSO4—Klebsiella pneumoniaemixture and CuSO4—K. pneumoniaephage mixture were added to the larval diet to analyze whetherK. pneumoniaecan protect housefly larvae against Cu2+injury. Our results showed that larval development was inhibited when were fed with CuSO4, the bacterial abundance ofProvidenciain the intestine of larvae increased. However, the inhibition effects of CuSO4was relieved whenK. pneumoniaemixed and added in larval diets, the abundance ofProvidenciadecreased. Electron microscope results revealed thatK. pneumoniaeshowed an obvious adsorption effect on copper ion in vitro.ConclusionsBased on the results we assume thatK. pneumoniaecould adsorb Cu2+, reduce Cu2+impact on gut community structure. Our study explains the role ofK. pneumoniaeantagonizing Cu2+, which could be applied as a probiotic to saprophytic bioantagonistic metal contamination.
Genome-wide transcriptional response to silver stress in extremely halophilic archaeon Haloferax alexandrinus DSM 27206 T
Authors: Buda Doriana Mădălina , Szekeres Edina , Tudoran Lucian Barbu , Esclapez Julia , Banciu Horia Leonard 
Published: December 2023

Abstract
Background
The extremely halophilic archaeon Haloferax (Hfx.) alexandrinus DSM 27206 T was previously documented for the ability to biosynthesize silver nanoparticles while mechanisms underlying its silver tolerance were overlooked. In the current study, we aimed to assess the transcriptional response of this haloarchaeon to varying concentrations of silver, seeking a comprehensive understanding of the molecular determinants underpinning its heavy metal tolerance.

Results
The growth curves confirmed the capacity of Hfx. alexandrinus to surmount silver stress, while the SEM–EDS analysis illustrated the presence of silver nanoparticles in cultures exposed to 0.5 mM silver nitrate. The RNA-Seq based transcriptomic analysis of Hfx. alexandrinus cells exposed to 0.1, 0.25, and 0.5 mM silver nitrate revealed the differential expression of multiple sets of genes potentially employed in heavy-metal stress response, genes mostly related to metal transporters, basic metabolism, oxidative stress response and cellular motility. The RT-qPCR analysis of selected transcripts was conducted to verify and validate the generated RNA-Seq data.

Conclusions
Our results indicated that copA, encoding the copper ATPase, is essential for the survival of Hfx. alexandrinus cells in silver-containing saline media. The silver-exposed cultures underwent several metabolic adjustments that enabled the activation of enzymes involved in the oxidative stress response and impairment of the cellular movement capacity. To our knowledge, this study represents the first comprehensive analysis of gene expression in halophillic archaea facing increased levels of heavy metals.
Phenotypic characterization and seed viability test in ex-situ conserved Ethiopian cultivated barley (Hordeum vulgare L.) landraces
Authors: Gadissa Fekadu , Gudeta Temesgen Bedassa 
Published: December 2023

Abstract
Background
Nowadays, most of the Ethiopian barley landraces had been lost from farmer’s field and exclusively found ex-situ conserved at the Ethiopian Biodiversity Institute (EBI). Those ex-situ conserved are generally believed to be representative of the original population and possess high genetic diversity and important unique genes that are useful for tackling the various biotic and abiotic stresses in the face of the current climate change. Thus, this research was aimed at testing the performance of 150 ex-situ conserved landraces that had been collected from Arsi and Bale highlands, Southeastern Ethiopia. The landraces were tested at multiple test locations over two years (2021 and 2022).

Results
All the tested landraces showed a good germination rate regardless of their long storage duration. In addition, performance of all the qualitative traits revealed a varying frequency for each character state. For example, most of the accessions (51.3%) had six kernel row numbers (KRN). All the remaining accessions had two rows (28.7%) and irregular KRN with variable lateral florets (20%). Likewise, some of the quantitative traits considered showed a significant variation among the landraces. However, there observed a significant variation for all the interaction effects in some of the traits considered signifying the importance of considering environment effects while targeting genetic selection and improvement of ex-situ conserved germplasms. The phenotypic coefficients of variation (PCV) were considerably high to medium in most of the traits considered including seed yield per hectare (SYPH) but with no associated higher genotypic coefficients of variation (GCV). Moreover, all the traits showed a far greater phenotypic coefficient of variation (PCV) to that of genotypic coefficients of variation (GCV) once again suggesting the pronounced effect of environmental factors to the variation. This was far supported by the significantly higher absolute magnitudes in phenotypic correlation compared to their corresponding genotypic correlation in most of the traits. Low estimates of heritability and genetic advance observed in all the traits considered except seed yield per hectare indicate importance of the trait for selection in Ethiopian barley improvement programs. Clustering patterns of the accessions, in narrow sense, revealed the existence of low divergence among the samples.

Conclusion
Ethiopian barley landraces are promising candidates for further yield improvement and conservation. However, further regular testing and screening should be conducted for the ex-situ conserved landraces because of the current erratic climate change. In addition, more robust molecular marker systems could be used to clearly reveal the extents of genetic diversity and to facilitate the breeding and conservation of Ethiopian barley landraces.
Analysis of hemi-uterus pregnancy outcomes in uterine malformations: a retrospective observational study
Authors: Qian Liang , Ding Jiashan , Shi Lan , Yu Qing , Zhu Jiawei , Huang Anfeng 
Published: December 2023

Abstract
Background
The association between uterine malformations and adverse pregnancy outcomes is well recognized. However, studies on adverse pregnancy outcomes based on one kind of anatomical commonality between different uterine anomalies have not been reported. This study aimed to investigate pregnancy outcomes in pregnancies with uterine malformations when the pregnancy is confined to a hemi-uterus.

Methods
A retrospective observational study of 336 women who gave birth at our hospital from 2015 to 2021 was performed. Women (n = 112) with a unicornuate, complete bicornuate, or didelphic uterus were set as the study group, and women (n = 224) with a normal uterus were set as the reference group. Maternal and neonatal outcomes were evaluated and compared between the two groups using Student’s t-test, one-way ANOVA, Chi-squared test, Yates correction for continuity, or Fisher’s exact test. Modified Poisson regression analyses were used to estimate the relationships between the hemi-uterus pregnancy and preterm birth, preterm premature rupture of membranes, and cesarean section rates by adjusting for potential confounders. A P value < 0.05 was considered significant.

Results
Women in the study group had a higher history of spontaneous abortion (24.1% vs. 10.7%, P = 0.002) and intrauterine fetal death (5.4% vs. 0.4, P = 0.006). Compared with the reference group, the study group had significantly higher rates of assisted reproductive technology (9.4% vs. 2.2%, P = 0.001) and cord-around-the neck (54.5% vs. 29.9%, P = 0.000). Modified Poisson regression analyses showed that the study group was at higher risk for preterm birth (aRR, 6.8; 95% CI 2.7–16.7), preterm premature rupture of membranes (aRR, 14.1; 95% CI 3.2–62.5), malpresentation (aRR, 13.2; 95% CI 6.3–27.7), and cesarean section (aRR, 4.4; 95% CI 3.3–5.7).

Conclusion
Women with a unicornuate, didelphic, or complete bicornuate uterus are at higher risk for some adverse pregnancy outcomes than those with a normal uterus.
Identification of a Novel CD8+ T cell exhaustion-related gene signature for predicting survival in hepatocellular carcinoma
Authors: Liu Kejun , Liu Junhao , Zhang Xusheng , Liu Di , Yao Weijie , Bu Yang , Chen Bendong 
Published: December 2023

Abstract
Background
Hepatocellular carcinoma (HCC) is a major health concern, necessitating a deeper understanding of its prognosis and underlying mechanisms. This study aimed to investigate the mechanism and prognostic value of CD8+ T Cell exhaustion (CD8+ TEX)-related genes in HCC and construct a survival prognosis prediction model for patients with HCC.

Methods
CD8+ TEX-related genes associated with HCC prognosis were analysed and identified, and a prognostic prediction model was constructed using the ‘least absolute shrinkage and selection operator’ Cox regression model. Immunohistochemistry was used to verify the expression of the model genes in HCC tissues. A nomogram was constructed based on risk scores and clinical features, and its predictive efficacy was verified. The expression of STAM, ANXA5, and MAD2L2 in HCC cell lines was detected by western blotting; subsequently, these genes were knocked down in HCC cell lines by small interfering RNA, and their effects on the proliferation and migration of HCC cell lines were detected by colony formation assay, cck8, wound healing, and transwell assays.

Results
Six genes related to CD8+ TEX were included in the risk-prediction model. The prognosis of patients with HCC in the low-risk group was significantly better than that of those in the high-risk group. Cox regression analysis revealed that the risk score was an independent risk factor for the prognosis of patients with HCC. The differentially expressed genes in patients with high-risk HCC were mainly enriched in the nucleotide-binding oligomerization domain-containing protein-like receptor, hypoxia-inducible factor-1, and tumour programmed cell death protein (PD)-1/PD-L1 immune checkpoint pathways. The CD8+ TEX-related genes STAM, ANXA5, and MAD2L2 were knocked down in HCC cell lines to significantly inhibit cell proliferation and migration. The prediction results of the nomogram based on the risk score showed a good fit and application value.

Conclusion
The prediction model based on CD8+ TEX-related genes can predict the prognosis of HCC and provide a theoretical basis for the early identification of patients with poor HCC prognosis.
Low-level laser therapy combined with scleral graft transplantation in the treatment of contracted socket: a clinical study
Authors: Huang Qin , Fang Yangbin , Lai Yao , Liao Hongfei 
Published: December 2023

Abstract
Objective
To analyse the efficacy of the therapeutic use of low-level laser therapy (LLLT) on the tissue repair process of allogeneic scleral grafts in patients with contracted sockets by analysing the speed of graft vascularisation and fornice depth of contraction percentage.

Methods
A retrospective chart review was performed from April 2015 to April 2021 including 39patients with socket contraction. Allogeneic scleral grafts were used to repair the sockets in all patients. They were randomly enrolled into two groups. The laser group included 18 patients treated with LLLT after the surgery, whereas the control group included 21 patients without LLLT after the surgery who healed naturally. The LLLT equipment used in the research had a wavelength of 650 nm, 10 mW power, and 3.8 J/cm2 dosimetry, and the procedure was performed once daily for 5 min over 7 days, beginning 1 week postoperatively. All patients were followed up over 6 months to examine the changes in the size of the area of the non-vascularised graft and upper and inferior fornice depth.

Results
The laser group presented a significantly increased speed of conjunctival vascularisation compared with the control group (P = 0.003). The fornice depth of contraction percentage was more apparent in the control group than that in the laser group (P = 0.000).

Conclusion
LLLT accelerates conjunctival vascularisation, stimulates conjunctival incision healing within a short period, shortens the tissue repair process, reduces the local inflammatory response, and causes no significant shrinkage of the conjunctival sac.
Management strategies of dental anxiety and uncooperative behaviors in children with Autism spectrum disorder
Authors: Tang Shi-Jun , Wei Hong-Lin , Li Cai-Yu , Huang Ming-Na 
Published: December 2023

Abstract
Background
Children with Autism spectrum disorder (ASD) was frequently experienced dental anxiety and uncooperative behaviors during dental treatment. Oral health care was necessary because of the poor oral hygiene and prevalent dental diseases in this population.

Aim
In this systematic review, we evaluated the effectiveness and feasibility for pediatric dentist to manage the dental anxiety in children with ASD.

Design
PubMed, Embase, and Cochrane Library were systematically performed on the literature search. The date of eligible publications was from inception to January 2023. After that, the quality of eligible studies was assessed by the Newcastle Ottawa Scale (NOS). Review findings were summarized using the PRISMA Statement for reporting.

Results
A total of six studies were systematically evaluated according to the inclusion and exclusion criteria. Five studies were conducted to evaluate ASD Children's anxiety and uncooperative performance in the progressive oral examination, oral disease prophylaxis and fluoride application. The other one study evaluated the success rate of treatment in decayed permanent tooth treatment. In the included studies, four studies indicated that it was extremely necessary to reduce dental anxiety of ASD children to increase the cooperation in sensory-adapted dental environment (SADE).

Conclusion
It is not always effective and feasible for pediatric dentist to manage the dental anxiety in children with autism during routine oral examination. Meanwhile, it is necessary for ASD children to conduct preoperative psychological assessment, to investigate parents’ expectations and cooperation, and to determine whether to start corresponding dental treatment.