bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimating the non-market value of protected areas: the case of Senkele Swayne Hartebeest Sanctuary, South Ethiopia
Authors: Wogayehu Dimim , Amanuel Desalegn , Tadie Getachew 
Published: November 2023

Abstract
Protected Areas provide secured habitat for plants and animals. The principal objective of the study was to estimate the determinants that influence households’ decision, maximum and the mean willingness to pay local rural farm for conservation and restoration of Senkele Swayne’s hartebeest sanctuary. To achieve the objective of the study, the contingent valuation method (CVM) was using double bound dichotomous questions followed by open-ended questions. Sample data from 175 rural households were collected by applying two stage sampling procedures. The primary data was collected using structured and semi-structured questionnaire interviews, conducting, a focus group discussion and a key informant interview. Relevant secondary data was also reviewed. Both descriptive statistics and econometric methods of analysis were used. Econometric models such as bivariate probit, binary probit and Tobit models were used to estimate mean WTP, determinants of decision WTP and the intensity of WTP respectively. The WTP result shows that the average amount of money from the open-ended format was 31.71 ETB per month and the mean WTP values from double bounded dichotomous households in the senkele sanctuary can pay up to 34.59 ETB per month. Determinants such as educational level, livestock holding and total land size had a positive and significant effect on the decision of households’ WTP. While distance from sanctuary and initial bid amount had a negative influence on households' WTP decisions. Therefore, the aggregated welfare gain expected from the conservation intervention of sanctuary from the open-ended format and double bounded dichotomous format were 12,024,812.52 ETB and 13,116,943.08 ETB per year respectively. Based on welfare gains from the sanctuary intervention, results show that the aggregate value of Sanctuary from an open-ended question format (12 million ETB) was underestimated as compared to a double bounded dichotomous choice format (13.12 million ETB). This indicates that there may be the existence of free riding problems in the study area. EWCA, researchers, local people and non-governmental organizations are working together to minimize deforestation and develop a sustainable management system for Senkele sanctuary.
A spatiotemporal comparative analysis on tumor immune microenvironment characteristics between neoadjuvant chemotherapy and preoperative immunotherapy for ESCC
Authors: Ba Yi , Zhou Zhengyang , Zhang Hongdian , Yang Jiayu , Wang Tongtong , Pan Wen , Zhang Qiumo , Wang Huiya , Tang Peng , Zhang Haiyang 
Published: November 2023

Abstract
The average five-year survival rate for esophageal cancer, a common malignant tumor of the digestive system, is barely 20%. The majority of esophageal squamous cell carcinoma (ESCC) patients had already progressed to a locally advanced or even advanced stage at initial diagnosis, making routine surgery ineffective. Chemotherapy and immunotherapy are important neoadjuvant treatments for ESCC, however, it remains unknown how treatment will affect the immunological microenvironment, especially at the spatial level. Here, we presented the TME characters of ESCC from the temporal and spatial dimensions using scRNA-seq and ST, investigated the changes of immune cell clusters in the TME under neoadjuvant chemotherapy and preoperative immunotherapy, and explored the potential mechanisms. It was found that compared with chemotherapy, immunotherapy combined with chemotherapy increased the level of T cell proliferation, partially restored the function of exhausted T cells, induced the expansion of specific exhausted CD8 T cells, increased the production of dendritic cells (DCs), and supported the immune hot microenvironment of the tumor. We also found that CD52 and ID3 have potential as biomarkers of ESCC. Particularly, CD52 may be served as a predictor of the efficacy to screen the advantaged population of different regimens. Through multiple pathways, CAF2 and CAF5's antigen-presenting role affected the other fibroblast clusters, resulting in malignant transformation. We analyzed the immune microenvironment differences between the two regimens to provide a more thorough description of the ESCC microenvironment profile and serve as a foundation for customized neoadjuvant treatment of ESCC.
Characterization of Plasmodium falciparum resistance genes to common antimalarials in semi-urban areas of Burkina Faso
Authors: Tchekounou Chanolle , Zida Adama , Soulama Issiaka , Zongo Cheikna , Sombie Salif , Nikiema seni , Yanogo Nassandba Julien , Sawadogo Salam , Kabore Farida Carelle Ariane , Zoure Oumou Aicha Zeina Djamila , Sawadogo Haffsatou , Sawadogo Patindoilba Marcel , Tibiri Yssimini Nadège Guillène , Guiguemde Kiswendsida Thierry , Ily Raissa Pousga , Ouedraogo-Traoré Rasmata , Ouedraogo Youssoufou , Savadogo Aly 
Published: November 2023

Abstract
Introduction: Malaria remains today the most frequent parasitic disease on the planet with, in 2021, 247 million cases and 619,000 deaths worldwide. Its control is compromised by the spread of the parasite's resistance to available antimalarials. The objective of our study is the characterization of Plasmodium falciparum resistance genes to common antimalarial drugs in semi-urban areas of Burkina Faso.
Material and Methods: This is a prospective cross-sectional study whose collection took place from June to October 2021 and from June to October 2022 in five health facilities in Burkina Faso. The molecular analysis took place from January to June 2023 at Centre National de Recherche et de Formation (CNRFP) to determine resistance genes such as Pfcrt, Pfmdr1, Pfdhps, and Pfdhfr. We have used the PCR-RFLP technique.
Results: On 150 samples analyzed we obtained prevalence of 46.67 %, 1.33 %, 0.67 %, 20%, 82%, and 4.67%, respectively for the mutations Pfcrt76T, Pfmdr1 86Y, Pfdhps 437G, Pfdhfr 51I, Pfdhfr 59R, and Pfdhfr 108N. There are no mutations observed for the mutant strains Pfdhps 540E and Pfdhfr 164L. However, the mutant strain Pfdhfr 59R was the most found and we also observed in general a triple mutation (Pfdhps 437G + Pfdhfr 59R + Pfdhfr 108N) with a low frequency which is 0.67%.
Conclusion: It would be wise to carry out increased surveillance of Plasmodium falciparum resistance markers to antimalarial drugs, one of the priorities in the context of the control or malaria elimination.
Evaluation of the Position of the Central Venous Catheter Tip of Implantable Venous Access Devices in the Occurrence of Postoperative Thrombotic and Non-Thrombotic Complications
Authors: Hamri Hicham , Pop C. Florin , Mauriac Sybil , Degreve Caroline , Khaled Charif , Vouche Michael , Moreau Michel , Liberale Gabriel 
Published: November 2023

Abstract

Background: The catheter tip of totally implanted venous access devices (TIVAD) is a risk factor for postoperative complications. The study aim was to assess the early (EC) and late complications (LC) associated with the position of the catheter tip in patients with cancer.
Methods: We reviewed cancer patients who had a TIVAD placed in 2020. EC (<90 days), LC (>90 days) and risk factors for TIVAD-associated complications were assessed. The vertical mismatch of the catheter tip compared to a “ideal position”: >10mm below the carina and >20 mm belowthe right main bronchus (RMB) was assessed on post-implantation chest x-ray.
Results:
301 patients were included, with a median time of port follow at 9.4 months. All TIVADs were inserted via the internal jugular vein (IJV). The mean distance between the catheter tip and the carina or RMB was 21.3 mm and6.63 mm respectively. In total, 11.3% TIVADs developed EC and 5.6% had LC. An association was found between the position of the catheter tip from the carina (≤ 10mm vs > 10mm) and the occurrence of EC (18.3% vs 8.6%, p=0.01) and for the insertion site (left IJV vs right IJV) (19.1% vs 9.0% p=0.02). In multivariate analysis, left IJV insertion (OR 2.76), and a catheter tip located ≤ 10 mm below the carina (OR 2.71) were the significant independent risk factors of EC.
Conclusions: TIVAD catheter tip located at less than 10 mm below the carina and a left-side inserted catheter are at higher risk of short-term complications.
Dynamic trajectories of life-threatening mass effect in patients with large middle cerebral artery stroke
Authors: Ong Charlene , Huang Qiuxi , Kim Ivy , Pohlmann Jack , Chatzidakis Stefanos , Brush Benjamin , Zhang Yihan , Du Yili , Mallinger Leigh Ann , Benjamin Emelia J. , Dupuis Josée , Greer David , Smirnakis Stelios , Trinquart Ludovic 
Published: November 2023

Abstract
Background
Life-threatening, space-occupying mass effect due to cerebral edema and/or hemorrhagic transformation is an early complication of patients with middle cerebral artery (MCA) stroke. Little is known about longitudinal trajectories of laboratory and vital signs leading up to radiographic and clinical deterioration related to this mass effect.
Methods
We curated a granular retrospective dataset of 635 patients with large middle cerebral artery (MCA) stroke totaling 108,547 data points for repeated measurements of 10 covariates, and 40 time-independent covariates. We assessed longitudinal trajectories of the 10 longitudinal variables during the 72 hours preceding three outcomes representative of life-threatening mass effect: midline shift (MLS) \(\ge\)5mm, pineal gland shift (PGS) \(>\)4mm, and decompressive hemicraniectomy (DHC). We used a “backward looking” trajectory approach. Patients were aligned according to the time of outcome occurrence and the trajectory of each variable was assessed prior to that outcome by accounting for both cases and non-cases.
Results
Of 635 patients, 49% were female, and mean age was 69 years. Thirty five percent of patients had MLS \(\ge\)5mm, 24.1% had PGS \(>\)4mm, and DHC occurred in 10.7%. For the three outcomes of interest, backward-looking trajectories showed mild increases in white blood cell count (10 up to 11 K/UL within 72 hours), temperature (up to half a degree within 24 hours), and sodium (1–3 mEq/L within 24 hours) leading up to outcomes. We also observed a decrease in heart rate (75 − 65 beats per minute) 24 hours prior to DHC.
Conclusions
Univariable longitudinal profiling showed that temperature, white blood cell count, and sodium increase prior to radiographic and clinical indicators of space-occupying mass effect. These findings will inform development of multivariable dynamic risk models to aid prediction of life-threatening space-occupying mass effect.
Structural studies of mononuclear yttrium and lutetium complexes bearing anthracenide and polyphenyl-substituted cyclopentadienyl ligands
Authors: Bardonov Daniil A. , Nasyrova Darina I. , Roitershtein Dmitrii M. , Lyssenko Konstantin A. , Minyaev Mikhail E. 
Published: November 2023

Abstract

Crystal structures of mononuclear cyclopentadienyl-anthracenide complexes [(1,3-Ph2C5H3)Lu(C14H10)(THF)2]•THF (1a), [(1,2,4-Ph3C5H2)Lu(C14H10)(THF)2]•0.17hexane•1.53THF (2a), [(1,2,4-Ph3C5H2)Lu(C14H10)(THF)2]•3C6D6 (2b), [(1,2,4-Ph3C5H2)(C14H10)Y(THF)2] •0.23hexane•1.37THF (3a), and [(1,2,4-Ph3C5H2)(2,6-tBu2C14H8)Ln(THF)2]•0.5THF (4a) have been studied. Complexes exhibit a structural rigidity of the (η5-С5)Ln(η2-С14)(O)2 crystallographic node. Electronic and steric effects are discussed. The cyclopentadienyl-anthracenide complexes are able to activate C = C bonds without a co-catalyst, using ethylene polymerization as an example.
A study of Fly-Ash and Slag Based Alkali Activated Binder Using Sea Water Based Alkaline Activator
Authors: Tadicharla V K Ratna Bhanu , D Gunneswara Rao T 
Published: November 2023

Abstract
This paper presents investigation in alkali activated paste and alkali activated mortar produced by combinations of flyash collected from coal based thermal power plant and ground granulated blast furnace slag collected from steel manufacturing facilities. The flyash and ground granulated blast furnace slag are industrial by products rich in aluminosilicates. The present study focuses on effect of utilization of seawater-based alkali activator solution in alkali activated paste and mortar. Alkali activator solution activates the chemical processes in binder materials when used in right concentrations and right proportion. This paper discusses the mechanical properties and microstructure characteristics of seawater-based alkali activated paste and mortar in comparison with conventional distilled water-based alkali activator. The usage of sea water shown insignificant change in setting times and compressive strengths for various combinations of binder material. The microstructure analysis also showed less variation in mineralogical formations when conventional distilled water is replaced with sea water in preparation of alkali activator solution.
Challenging pitfalls in frozen section pathology: A case of mandible ghost cell odontogenic carcinoma and the literature review
Authors: Hu Sha-sha , Yang Jian , Zhang Hai-Fei , Chen Jie , Li Xin-Nian , Liu Fu-Jin , Wang Bo 
Published: November 2023

Abstract
Background
Ghost cell odontogenic carcinoma (GCOC) is a rare malignancy characterized by the presence of ghost cells, preferably in the maxilla. Only slightly more than 50 case reports of GCOC have been documented to date. Given the rarity and nonspecific clinical criteria of the tumor, clinical examination, imaging findings and pathology are also prone to misdiagnosis.
Case presentation:
A 50-year-old male patient presented to the hospital due to experiencing pain in his lower front teeth while eating for the past 2 months. Upon specialist examination, a red, hard, painless mass was found in his left lower jaw, measuring approximately 4.0 cm × 3.5 cm. Based on the malignant histological morphology of the tumor and the abundant red-stained keratinized material, the preoperative frozen section pathology misdiagnosed it as squamous cell carcinoma (SCC). The surgical resection specimen pathology via paraffin section revealed that the tumor was characterized by round-like epithelial islands within the fibrous interstitium, accompanied by a large number of ghost cells and some dysplastic dentin with infiltrative growth. The malignant components displayed marked heterogeneity and mitotic activity. Additionally, a calcified cystic tumor component of odontogenic origin was observed. Hemorrhage, necrosis, and calcifications were present, with a foreign body reaction around ghost cells. Immunoreactivity for β-catenin showed strong nuclear positivity in tumor cells, while immunostaining was completely negative for p53. The Ki67 proliferation index was approximately 30–40%. The tumor cells exhibited diffuse CK5/6, p63, and p40 immunoreactivity, with varying immunopositivity for EMA. Furthermore, no BRAFV600E mutation was identified by ARMS-PCR. The final pathology confirmed that the tumor was a mandible GCOC.
Conclusion
We have reported and summarized for the first time the specific manifestations of GCOC in frozen section pathology and possible pitfalls in misdiagnosis. We also reviewed and summarized the etiology, pathological features, molecular characteristics, differential diagnosis, imaging features, and current main treatment options for GCOC. Due to its rarity, the diagnosis and treatment of this disease still face certain challenges. A correct understanding of the pathological morphology of GCOC, distinguishing the ghost cells and the secondary stromal reaction around them, is crucial for reducing misdiagnosis rates.
Correlation between Craniocervical Posture and Upper Airway Dimension in Patients with Bilateral Anterial Disc Displacement
Authors: Xiang Wanfang , Wang Min , Cai Mingqin , Li Zhihui , Hou Binjie , Pan Xiaojing 
Published: November 2023

Abstract
Objective
To investigate the relationship between upper airway dimension and craniocervical posture in adult patients with bilateral anterior disc displacement and to provide some references for clinical diagnosis and plan formulation in orthodontics.
Methods
Based on RDC/TMD diagnostic criteria of two experienced TMJ specialists, 98 Patients were divided into three groups: bilateral disc normal position group (BN), bilateral anterior disc displacement with reduction group (ADDWR) and bilateral anterior disc displacement without reduction group (ADDWoR). Inter-group comparison and correlation analysis were performed after 11 craniocervical posture and 15 upper airway dimension items were measured by Dolphin and Uceph software in Two or Three-dimensional.
Results
Anterior disc displacement often accompanied with tilt and extension of craniocervical posture, as ADDWR or ADDWoR have significantly higher cervical curvature and inclination than BN group. Simultaneously anterior disc displacement often associated with constrained upper airway dimension for the total or each segment volume of upper airway and the minimum cross-sectional sagittal diameter of glossopharynx were significantly smaller in ADDWR or ADDWoR than BN group. Correlation analysis revealed that C0-C1 (the distance from the base of the occipital bone (C0) to the posterior arch of the atlas (C1)) is significantly related to the total and each segment upper airway volume.
Conclusion
There exists close correlation between anterior disc displacement and craniocervical posture forward extension, which may be physiologically adaptive cervical extension to keep oropharyngeal airway unobstructed as upper airway dimension constrained by anterior disc displacement.
Clinical relevance
These findings allow us to infer the potential consequences if the treatment of anterior disc displacement would result in an improvement of intervertebral relationships and upper airway constraint.
New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out in the first generation using an optimized zCas9i
Authors: Villette Jérémy , Lecourieux Fatma , Bastiancig Eliot , Héloir Marie-Claire , Poinssot Benoit 
Published: November 2023

Abstract
Background: For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up a wide range of possibilities for improved plant breeding in the future. In plants, the CRISPR/Cas9 system is mostly used through stable transformation with constructs that allow for the expression of the Cas9 gene and sgRNA. Numerous studies have shown that site-specific mutation efficiency can vary greatly between different plant species due to factors such as plant transformation efficiency, Cas9 expression, Cas9 nucleotide sequence, the addition of intronic sequences, and many other parameters. Since 2016, when the first edited grapevine was created, the number of studies using functional genomic approaches in grapevine has remained low due to difficulties with plant transformation and gene editing efficiency. In this study, we optimized various aspects of the process to obtain site-specific mutations and generate knock-out mutants of grapevine (Vitis vinifera cv. ‘Chardonnay’). Building on existing methods of grapevine transformation, we improved the method for selecting transformed plants at chosen steps of the developing process using fluorescence microscopy.
Results: By comparison of two different Cas9 gene and two different promoters, we increased site-specific mutation efficiency using a maize-codon optimized Cas9 containing 13 introns (zCas9i), achieving up to 100% biallelic mutation in the first generation of grapevine seedlings cv. ‘Chardonnay’. These results are directly correlated with Cas9 expression level.
Conclusions:Taken together, our results highlight a complete methodology for obtaining a wide range of homozygous knock-out mutants within 9 months for functional genomic studies and future breeding programs in grapevine.
Use and predictors of over-the-counter medication among pregnant women visiting antenatal clinics in public health facilities in the Silte Zone, Central Ethiopia
Authors: Mosa Hassen , Muze Mohammed , Abdella Bahredin , Jemal Musa , Arusi Muhudin 
Published: November 2023

Abstract

Background
Self-medication with over-the-counter (OTC) drugs during pregnancy is a global problem that needs to be given significant attention due to the potential risks to both the mother and the foetus. However, data regarding the use and risk factors for OTC drugs during pregnancy are lacking in the study area. The purpose of this study was to assess OTC medications and predictors among pregnant women attending antenatal care (ANC) at selected public health facilities in the Silte Zone, Central Ethiopia.
Methods
An institution-based cross-sectional study was carried out from February 1st to 30th, 2023. A multistage sampling technique was used to select study participants. Data were collected by KoboTool box and analysed using SPSS (version 25). The association of independent variables with the dependent variable was determined using logistic regression analysis. To decide the presence and strength of an association, odds ratios (ORs) with their 95% confidence intervals (CIs) were calculated. Findings were considered statistically significant when the p value was < 0.05.
Results
The prevalence of OTC medicine use was reported among 297 (55.2%) pregnant women during their current pregnancy. Age ≥ 35 (AOR = 12.6, 95% CI = 6.3–25.0), living in rural areas (AOR = 7.1, 95% CI = 4.1–12.3), not having health insurance (AOR = 17, 95% CI = 9, 9.3–31.3), having poor knowledge about the possible risks of OTC medication in the fetus (AOR = 4.6, 95% CI = 5.3–12.8), and attending fewer than four ANC visits (AOR = 9.5, 95% CI = 4.7–19.0) were predictors of OTC medicine use
Conclusion
This study revealed that a significant number of pregnant women used one or more OTC drugs. Pregnant women who are older, live in rural areas, lack health insurance, have less knowledge, and attend fewer ANC visits are more likely to use OTC drugs. Therefore, healthcare providers should offer advice to pregnant women about the safe use of OTC medications. This is especially important for older women who live in rural areas, have no health insurance, have low knowledge, and fail to frequently attend ANC visits.