bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rare presentation of measles and post-measles complications in a neonate: case report
Authors: Sharma Suraksha Ramakrishna , Sawant Vishal , Save Sushma Uttam , Kondekar Alpana Santosh 
Published: December 2023

Abstract
Background
Measles is a common infection in children. Measles infection in children less than 6 months of age is very rare since maternal antibodies play a major role in the prevention of the disease.

Case presentation
Here we present a rare case of a 34-day-old infant, who had a history of fever with a rash on the 18th day of life and significant history of measles contact with mother and elder brother. The patient presented to us with septic shock and multiorgan involvement in the form of pneumonia, acute gastroenteritis with severe dehydration, mucopurulent conjunctivitis, and post-measles encephalitis. The patient had a strong measles contact and epidemiological linkage to the measles epicenter, Govandi (a locality in the Mumbai region of western India), so measles IgM antibody was obtained which were significantly raised.
The patient was successfully revived from this critical presentation with early and appropriate resuscitation, timely ionotropic support, and the use of empirical broad-spectrum antibiotics. The patient required intensive unit care for four days and was started on feeds and discharged after 12 days of hospital stay. On follow-up, the patient is doing well and gaining weight.

Conclusion
Uncommon presentation and the epidemiology of the disease should alert the clinician of having a high index of suspicion. Early intervention and prompt treatment can help reduce morbidity and mortality.
Dynamics behavior of PE and PET oligomers in lipid bilayer simulations
Authors: Järvenpää Joni P. , Lahtela-Kakkonen Maija K. 
Published: December 2023

AbstractIn recent years many investigators have been concerned about the toxicity and potential health hazards of micro- and nanoplastics. However, we are still lacking a good understanding of the methods of their transport into the human body and subsequently within cells. This is especially true at the lower nanometer scale; these particles are potentially more dangerous than their micrometer counterparts due to their easier permeation into cells. In this study we used both unbiased molecular dynamics simulations and steered umbrella sampling simulations to explore the interactions of polyethylene terephthalate (PET) and polyethylene (PE) oligomers in phospholipid bilayers. Our simulations revealed that the bilayers did not represent significant energy barriers to the small oligomers; not only did they readily enter the cell membrane but they also became concentrated into specific parts of the membrane. The larger PET tetramers exhibited a strong aggregation in water but were the least likely to permeate through or into the membranes. It is possible that PE monomers and tetramers can become concentrated into membranes while PET monomers are more likely to pass through or concentrate just inside the membrane surface. Passive transport of microplastics into cells is, however, likely limited to particles of a few nanometers in diameter.
Utility of Tumor Mutational Burden as a Biomarker for Response to Immune Checkpoint Inhibition in the VA Population
Authors: Scobie Micaela R. , Zhou Katherine I. , Ahmed Sara , Kelley Michael J. 
Published: December 2023

PURPOSE Immune checkpoint inhibitors (ICIs) are used for an increasing number of indications across various tumor types, as well as several tumor-agnostic indications in patients with advanced cancer. Although many patients benefit from ICI therapy, others do not, highlighting a need for better predictive biomarkers. Tumor mutational burden (TMB) reflects the global number of mutations within a tumor and has been widely explored as a predictive biomarker of ICI response. The current tumor type–agnostic US Food and Drug Administration approval of pembrolizumab for metastatic solid tumors defines high TMB (TMB-H) as ≥10 mut/Mb as measured by FoundationOne CDx. This fixed cutoff may not be the ideal value across all solid tumors. METHODS We performed a retrospective analysis of the association of survival outcomes with TMB in patients treated with ICI for five major cancer types, using real-world data from the VA. Survival was measured from initiation of ICI, and Kaplan-Meier survival curves were compared by log-rank test. RESULTS Overall survival (OS) was significantly longer for patients with TMB-H versus TMB low tumors in non–small-cell lung cancer (NSCLC; n = 1,593), head and neck (H&N) cancer (n = 222), and urothelial cancer (n = 332). OS was not significantly different based on TMB status in melanoma (n = 207) or esophageal/gastric cancer (n = 248). CONCLUSION Consistent with previous studies, a predictive value of TMB ≥10 mut/Mb for ICI response was found in NSCLC and H&N, but not in esophageal/gastric cancer. Although inconclusive in the literature, significant association was found in urothelial cancer. The predictive value of TMB in melanoma was inconclusive. Our analysis does not support the use of a fixed threshold for TMB as a standalone predictive biomarker for ICI across all solid tumors.
Comprehensive Long-Read Sequencing Analysis Discloses the Transcriptome Features of Papillary Thyroid Microcarcinoma
Authors: Wang Yanqiang , Zou Binbin , Zhang Yanyan , Zhang Jin , Li Shujing , Yu Bo , An Zhekun , Li Lei , Cui Siqian , Zhang Yutong , Yao Jiali , Shi Xiuzhi , Liu Jing 
Published: December 2023

Abstract

Context
Papillary thyroid microcarcinoma (PTMC) is the most common type of thyroid cancer. It has been shown that lymph node metastasis is associated with poor prognosis in patients with PTMC.

Objective
We aim to characterize the PTMC transcriptome landscape and identify the candidate transcripts that are associated with lateral neck lymph node metastasis of PTMC.

Methods
We performed full-length transcriptome sequencing in 64 PTMC samples. Standard bioinformatic pipelines were applied to characterize and annotate the full-length expression profiles of 2 PTMC subtypes. Functional open reading frame (ORF) annotation of the known and novel transcripts were predicted by HMMER, DeepLoc, and DeepTMHMM tools. Candidate transcripts associated with the pN1b subtype were identified after transcript quantification and differential gene expression analyses.

Results
We found that skipping exons accounted for the more than 27.82% of the alternative splicing events. At least 42.56% of the discovered transcripts were novel isoforms of annotated genes. A total of 39 193 ORFs in novel transcripts and 18 596 ORFs in known transcripts were identified. Distribution patterns of the characterized transcripts in functional domain, subcellular localization, and transmembrane structure were predicted. In total, 1033 and 1204 differentially expressed genes were identified in the pN0 and pN1b groups, respectively. Moreover, novel isoforms of FRMD3, NOD1, and SHROOM4 were highlighted for their association with pN1b subtype.

Conclusion
Our data provided the global transcriptome landscape of PTMC and also revealed the novel isoforms that associated with PTMC aggressiveness.
Elevated Norepinephrine Stimulates Adipocyte Hyperplasia in Ovine Fetuses With Placental Insufficiency and IUGR
Authors: Luna-Ramirez Rosa I , Kelly Amy C , Anderson Miranda J , Bidwell Christopher A , Goyal Ravi , Limesand Sean W 
Published: December 2023

Abstract
Prevailing hypoxemia and hypoglycemia in near-term fetuses with placental insufficiency-induced intrauterine growth restriction (IUGR) chronically increases norepinephrine concentrations, which lower adrenergic sensitivity and lipid mobilization postnatally, indicating a predisposition for adiposity. To determine adrenergic-induced responses, we examined the perirenal adipose tissue transcriptome from IUGR fetuses with or without hypercatecholaminemia. IUGR was induced in sheep with maternal hyperthermia, and hypercatecholaminemia in IUGR was prevented with bilateral adrenal demedullation. Adipose tissue was collected from sham-operated control (CON) and IUGR fetuses and adrenal-demedullated control (CAD) and IUGR (IAD) fetuses. Norepinephrine concentrations were lower in IAD fetuses than in IUGR fetuses despite both being hypoxemic and hypoglycemic. In IUGR fetuses, perirenal adipose tissue mass relative to body mass was greater compared with the CON, adrenal-demedullated control, and IAD groups. Transcriptomic analysis identified 581 differentially expressed genes (DEGs) in CON vs IUGR adipose tissue and 193 DEGs in IUGR vs IAD adipose tissue. Integrated functional analysis of these 2 comparisons showed enrichment for proliferator-activated receptor signaling and metabolic pathways and identified adrenergic responsive genes. Within the adrenergic-regulated DEGs, we identified transcripts that regulate adipocyte proliferation and differentiation: adipogenesis regulatory factor, C/CCAAT/enhancer binding protein α, and sterol carrier protein 2. DEGs associated with the metabolic pathway included pyruvate dehydrogenase kinase 4, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4, IGF-binding proteins (IGFBP-5 and IGFBP-7). Sex-specific expression differences were also found for adipogenesis regulatory factor, pyruvate dehydrogenase kinase 4, IGFBP5, and IGFBP7. These findings indicate that sustained adrenergic stimulation during IUGR leads to adipocyte hyperplasia with alterations in metabolism, proliferation, and preadipocyte differentiation pathways.
Anticorruption, Development, and the Indian State
Authors: Balasubramanian Aditya 
Published: December 2023

Abstract
This essay studies the evolution of India's anticorruption machinery and discourses of corruption from the 1940s to the 1960s as a tussle between the logics of bureaucracy and democracy playing out as part of decolonization. Anticorruption began as a way of safeguarding the British Government of India's war effort from wayward officials pilfering supplies. As the postcolonial government retooled coercive wartime laws and empowered an increasing number of bureaucrats to manage the economy for developmental purposes, anticorruption evolved into a key demonstration of accountability by the state to the Indian people. Meanwhile, discourses of corruption became increasingly politically potent as India's imperial subjects became voting citizens. These discourses created pressure for a reform of the anticorruption machinery. But the mechanisms resisted full democratization and continued to privilege the executive branch as they had in colonial times. Anticorruption mechanisms became used to subdue challenges to power that took place. They were themselves corrupted.
Asia in a Fragile World
Authors: Phongpaichit Pasuk 
Published: December 2023

Abstract
This keynote address offers an overview of Asia in an increasingly fragile world. Climate change is making the monsoon more erratic, bringing more drought and more flood, signaled by the devastating flood of Pakistan in August 2022. Extreme weather and rising sea levels are increasing the “human flow” of migrants, especially into Asia's megacities. Climate change reacts with other trends: the increased inequalities willed by a generation of neoliberal policies, the authoritarian turn in politics, and the return of geopolitical tensions resulting from China's spectacular rise. More dialogue between the sciences and the social sciences is needed to address these multiple fragilities.
Kingpins at Court
Authors: Kang Hyeok Hweon 
Published: December 2023

Abstract
In the mid-seventeenth century, the Chosŏn Korean (1392–1910) court ran a smuggling enterprise in Japan, using both formal diplomatic agents and private merchants to import military contraband. This royal enterprise caused the largest documented criminal investigation in early modern Japan, complicating diplomatic relations with both the Tokugawa shogunate (1603–1868) and the daimyos of its Tsushima domain. By examining this history of “contraband diplomacy,” this article intervenes in the study of early modern diplomacy and imperialism. Contrary to scholarly portrayals of East Asian polities as isolationist and uninterested in trade, it shows Korean kings and officials aggressively pursuing commerce. If previously misunderstood as “self-organized,” their efforts reveal a different aspect of state formation in the global early modern period. Without becoming imperial or mercantilist, some institutions, such as those of Chosŏn and Tsushima, spearheaded international criminal activities, and others—like the shogunate—exercised growing administrative intent and capacity to stymie them.
The centrosome – diverse functions in fertilization and development across species
Authors: Aljiboury Abrar , Hehnly Heidi 
Published: December 2023

ABSTRACT
The centrosome is a non-membrane-bound organelle that is conserved across most animal cells and serves various functions throughout the cell cycle. In dividing cells, the centrosome is known as the spindle pole and nucleates a robust microtubule spindle to separate genetic material equally into two daughter cells. In non-dividing cells, the mother centriole, a substructure of the centrosome, matures into a basal body and nucleates cilia, which acts as a signal-transducing antenna. The functions of centrosomes and their substructures are important for embryonic development and have been studied extensively using in vitro mammalian cell culture or in vivo using invertebrate models. However, there are considerable differences in the composition and functions of centrosomes during different aspects of vertebrate development, and these are less studied. In this Review, we discuss the roles played by centrosomes, highlighting conserved and divergent features across species, particularly during fertilization and embryonic development.
End-of-life care in the patient's home
Authors: Manyimo Pretty , de Vries Kay 
Published: December 2023

Background: Providing end-of-life care in a patient's home is challenging, especially if the nurse is from a different ethnic background from the patient. Evidence shows that people from an ethnic minority background often experience poorer end-of-life care due to care providers not understanding their care needs. Aim: To explore the experiences of community nursing staff caring for patients with an ethnic minority background at the end of their life. Method: Using a qualitative descriptive approach, 16 semi-structured interviews were conducted and the data was thematically analysed. Finding: Participants dealt with large tight-knit families, paternalism and controlling behaviours. There was a mistrust of Western palliative medicine. Language barriers and challenges were faced by the nursing staff, resulting in difficult conversations with the families. Different levels of the nursing staff's spiritual/cultural competencies were also identified. The COVID-19 pandemic intensified the challenges. Conclusion: This research has shed light on the challenges of individualising end-of-life care in a culturally diverse community in the UK. The nursing staff's cultural/spiritual competencies were challenged.
In situ fixation of slipped capital femoral epiphysis carries a greater than 40% risk of later total hip arthroplasty during a long-term follow-up
Authors: Schlenzka Thomas , Serlo Joni , Viljakka Timo , Tallroth Kaj , Helenius Ilkka 
Published: December 2023

AimsWe aimed to assess the cumulative risk of total hip arthroplasty (THA) from in situ fixation for slipped capital femoral epiphysis (SCFE) after a follow-up of almost 50 years.MethodsIn this study, 138 patients with 172 affected hips treated with in situ fixation were evaluated retrospectively. A total of 97 patients (70%) were male and the mean age was 13.6 years (SD 2.1); 35 patients (25%) had a bilateral disease. The median follow-up time was 49 years (interquartile range 43 to 55). Basic demographic, stability, and surgical details were obtained from patient records. Preoperative radiographs (slip angle; SA) were measured, and data on THA was gathered from the Finnish National Arthroplasty Register.ResultsThe preoperative SA was a mean of 39° (SD 19°). At follow-up, 56 of the patients had undergone THA for a hip previously fixed in situ for SCFE (41%) and 64 of all affected hips had been replaced (37%). Kaplan-Meier analysis gave a median prosthesis-free postoperative survival of 55 years (95% confidence interval (CI) 45 to 64) for the affected hips. In a multivariate analysis, female patients had a two-fold risk for THA (hazard ratio (HR) 2.42 (95% CI 1.16 to 5.07)) and a greater preoperative SA increased the risk of THA (HR 1.03 for every increment of 1° (95% CI 1.01 to 1.05)), while patient age at surgery, slip laterality, stability of slip, or diagnostic delay did not have a statistically significant effect on the risk of THA.ConclusionSCFE treated primarily with in situ fixation may lead to THA in more than 40% of affected hips at a near 50-year follow-up. This risk is approximately 15-times the reported lifetime risk in the Finnish general population. Female sex and increasing preoperative SA significantly predicted higher risk of THA.Cite this article: Bone Joint J 2023;105-B(12):1321–1326.
Does brace treatment following closed reduction of developmental dysplasia of the hip improve acetabular coverage?
Authors: Morris William Z. , Kak Arnav , Mayfield Laura M. , Kang Michael S. , Jo Chan-Hee , Kim Harry K. W. 
Published: December 2023

AimsAbduction bracing is commonly used to treat developmental dysplasia of the hip (DDH) following closed reduction and spica casting, with little evidence to support or refute this practice. The purpose of this study was to determine the efficacy of abduction bracing after closed reduction in improving acetabular index (AI) and reducing secondary surgery for residual hip dysplasia.MethodsWe performed a retrospective review of patients treated with closed reduction for DDH at a single tertiary referral centre. Demographic data were obtained including severity of dislocation based on the International Hip Dysplasia Institute (IHDI) classification, age at reduction, and casting duration. Patients were prescribed no abduction bracing, part-time, or full-time wear post-reduction and casting. AI measurements were obtained immediately upon cast removal and from two- and four-year follow-up radiographs.ResultsA total of 243 hips underwent closed reduction and 82% (199/243) were treated with abduction bracing. There was no difference between those treated with or without bracing with regard to sex, age at reduction, severity of dislocation, spica duration, or immediate post-casting AI (all p > 0.05). There was no difference in hips treated with or without abduction brace with regard to AI at two years post-reduction (32.4° (SD 5.3°) vs 30.9° (SD 4.6°), respectively; p = 0.099) or at four years post-reduction (26.4° (SD 5.2°) vs 25.4° (SD 5.1°), respectively; p = 0.231). Multivariate analysis revealed only IHDI grade predicted AI at two years post-reduction (p = 0.004). There was no difference in overall rate of secondary surgery for residual dysplasia between hips treated with or without bracing (32% vs 39%, respectively; p = 0.372). However, there was an increased risk of early secondary surgery (< two years post-reduction) in the non-braced group (11.4% vs 2.5%; p = 0.019).ConclusionAbduction bracing following closed reduction for DDH treatment is not associated with decreased residual dysplasia at two or four years post-reduction but may reduce rates of early secondary surgery. A prospective study is indicated to provide more definitive recommendations.Cite this article: Bone Joint J 2023;105-B(12):1327–1332.
High treatment failure rate in haematogenous compared to non-haematogenous periprosthetic joint infection
Authors: Knoll Leonard , Steppacher Simon D. , Furrer Hansjakob , Thurnheer-Zürcher Maria C. , Renz Nora 
Published: December 2023

AimsA higher failure rate has been reported in haematogenous periprosthetic joint infection (PJI) compared to non-haematogenous PJI. The reason for this difference is unknown. We investigated the outcome of haematogenous and non-haematogenous PJI to analyze the risk factors for failure in both groups of patients.MethodsEpisodes of knee or hip PJI (defined by the European Bone and Joint Infection Society criteria) treated at our institution between January 2015 and October 2020 were included in a retrospective PJI cohort. Episodes with a follow-up of > one year were stratified by route of infection into haematogenous and non-haematogenous PJI. Probability of failure-free survival was estimated using the Kaplan-Meier method, and compared between groups using log-rank test. Univariate and multivariate analysis was applied to assess risk factors for failure.ResultsA total of 305 PJI episodes (174 hips, 131 knees) were allocated to the haematogenous (n = 146) or the non-haematogenous group (n = 159). Among monomicrobial infections, Staphylococcus aureus was the dominant pathogen in haematogenous PJI (76/140, 54%) and coagulase-negative staphylococci in non-haematogenous PJI (57/133, 43%). In both groups, multi-stage exchange (n = 55 (38%) in haematogenous and n = 73 (46%) in non-haematogenous PJI) and prosthesis retention (n = 70 (48%) in haematogenous and n = 48 (30%) in non-haematogenous PJI) were the most common surgical strategies. Median duration of antimicrobial treatment was 13.5 weeks (range, 0.5 to 218 weeks) and similar in both groups. After six years of follow-up, the probability of failure-free survival was significantly lower in haematogenous compared to non-haematogenous PJI (55% vs 74%; p = 0.021). Infection-related mortality was significantly higher in haematogenous than non-haematogenous PJI (7% vs 0% episodes; p = 0.001). Pathogenesis of failure was similar in both groups. Retention of the prosthesis was the only independent risk factor for failure in multivariate analysis in both groups.ConclusionTreatment failure was significantly higher in haematogenous compared to non-haematogenous PJI. Retention of the prosthesis was the only independent risk factor for failure in both groups.Cite this article: Bone Joint J 2023;105-B(12):1294–1302.