bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Poria cocos and Alismatis rhizoma against diet-induced hyperlipidemia in rats based on transcriptome sequencing analysis
Authors: Zhou Xiaowen , Luo Jingbiao , Lin Shuxian , Wang Yaxin , Yan Zhenqian , Ren Qi , Liu Xiaoqi , Li Xiantao 
Published: October 2023

AbstractHyperlipidemia, a common metabolic disease, is a risk factor for cardiovascular diseases, Poria cocos (PC) and Alismatis rhizoma (AR) serve as a potential treatment. A systematic approach based on transcriptome sequencing analysis and bioinformatics methods was developed to explore the synergistic effects of PC–AR and identify major compounds and potential targets. The phenotypic characteristics results indicated that the high dose (4.54 g/kg) of PC–AR reduced total cholesterol (TC), elevated high-density lipoprotein cholesterol (HDL-C) levels, and improved hepatocyte morphology, as assessed via hematoxylin and eosin (H&E) staining. Transcriptomic profiling processing results combined with GO enrichment analysis to identify the overlapping genes were associated with inflammatory responses. The cytokine-cytokine receptor interaction pathway was found as a potential key pathway using geneset enrichment analysis. Core enrichment targets were selected according to the PC–AR's fold change versus the model. Real-time quantitative PCR analysis validated that PC–AR significantly downregulated the expression of Cxcl10, Ccl2, Ccl4, Cd40 and Il-1β mRNA (P < 0.05). Molecular docking analysis revealed the significant compounds of PC–AR and the potential binding patterns of the critical compounds and targets. This study provides further evidence that the therapeutic effects of PC–AR on hyperlipidemia in rats through the regulation of inflammation-related targets.
Hydrothermal extraction and physicochemical characterization of biogenic hydroxyapatite nanoparticles from buffalo waste bones for in vivo xenograft in experimental rats
Authors: Alsharif Shada A. , Badran Mahmoud I. , Moustafa Moustafa H. , Meshref Radwa A. , Mohamed Ehab I. 
Published: October 2023

AbstractHydroxyapatite (HA) can be used in odontology and orthopedic grafts to restore damaged bone due to its stable chemical characteristics, composition, and crystal structural affinity for human bone. A three-step hydrothermal method was used for the extraction of biogenic calcined HA from the buffalo waste bones at 700 °C (HA-700) and 1000 °C (HA-1000). Extracts were analyzed by thermogravimetric analysis, differential scanning calorimetry, X-ray fluorescence, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and in vivo examination of HA xenografts for femoral lesions in experimental rats. Crystallinity, purity, and morphology patterns showed that the HA main phase purity was 84.68% for HA-700 and 88.99% for HA-1000. Spherical HA nanoparticles were present for calcined HA-700 samples in the range 57–423 nm. Rats with critical bone lesions of 3 mm in diameter in the left femur treated with calcined HA-700 nanoparticles healed significantly (p < 0.001) faster than rats treated with HA-1000 or negative controls. These findings showed that spherical biogenic HA-700 NPs with a bud-like structure have the potential to stimulate both osteoconduction and bone remodeling, leading to greater bone formation potential in vivo. Thus, the calcined biogenic HA generated from buffalo waste bones may be a practical tool for biomedical applications.
Improved structure of Zr-BTC metal organic framework using NH2 to enhance CO2 adsorption performance
Authors: Esfahani Heidar Javdani , Shahhosseini Shahrokh , Ghaemi Ahad 
Published: October 2023

AbstractModified mesoporous NH2-Zr-BTC mixed ligand MOF nanocomposites were synthesized via the hydrothermal method as a novel adsorbent for CO2 capture. The newly modified MOF-808 with NH2 demonstrated a similar mesoporous morphology as MOF-808, whereas the specific surface area, pore volume, and average particle size, respectively, increased by 15%, 6%, and 46% compared to those of MOF-808. The characterization analyses exhibited the formation of more active groups on the adsorbent surface after modification. In addition, a laboratory adsorption setup was used to evaluate the effect of temperature, pressure, and NH2 content on the CO2 adsorption capacity in the range of 25–65 °C, 1–9 bar, and 0–20 wt%, respectively. An increase in pressure and a decrease in temperature enhanced the adsorption capacity. The highest equilibrium adsorption capacity of 369.11 mg/g was achieved at 25 °C, 9 bar, and 20 wt% NH2. By adding 20 wt% NH2, the maximum adsorption capacity calculated by the Langmuir model increased by about 4% compared to that of pure MOF-808. Moreover, Ritchie second-order and Sips models were the best-fitted models to predict the kinetics and isotherm data of CO2 adsorption capacity with the high correlation coefficient (R2 > 0.99) and AARE% of less than 0.1. The ΔH°, ΔS°, and ΔG° values were − 17.360 kJ/mol, − 0.028 kJ/mol K, and − 8.975 kJ/mol, respectively, demonstrating a spontaneous, exothermic, and physical adsorption process. Furthermore, the capacity of MH-20% sample decreased from 279.05 to 257.56 mg/g after 15 cycles, verifying excellent stability of the prepared mix-ligand MOF sorbent.
Characterization of type-specific HPV prevalence in a population of persistent cutaneous warts in Flanders, Belgium
Authors: Redzic Nina , Pereira A. Rita , Menon Sonia , Bogers Johannes , Coppens Astrid , Kehoe Kaat , Vanden Broeck Davy 
Published: October 2023

AbstractCutaneous warts are benign skin lesions caused by the human papillomavirus (HPV). Even though they are considered benign, they can have a considerable impact on the quality of life and cause serious illness in certain immunocompromised populations. Studies have shown that the efficacy of wart treatment is dependent on the causative HPV type. Therefore, in this article, we aim to determine the HPV genotype-specific prevalence in cutaneous warts of a Flemish population as part of the Omnivirol-Salycilic acid randomized controlled trial. Swab samples of cutaneous warts (n = 269) were collected during enrollment. The DNA extraction was performed on the automated NucliSENS® easyMAG® system (bioMérieux). The samples were analyzed with two separate in-house PCR assays capable of detecting the most prevalent cutaneous HPV types (i.e. wart-associated HPV qPCR) as well as the most relevant mucosal types (i.e. RIATOL qPCR assay). In total, the type-specific prevalence of 30 distinct HPV genotypes was determined. The beta-globin gene was used as a cellularity control and for viral load quantification. Data concerning wart persistence, previous treatment, wart type, and other relevant wart and patient characteristics was collected through a baseline questionnaire. The study population consisted mostly of persistent warts considering that 98% (n = 263) of the sampled skin lesions were older than six months and 92% (n = 247) had undergone previous treatment. The most prominent wart type was the mosaic verruca plantaris (42%, n = 113). The most prevalent HPV types were cutaneous HPV types 27 (73%, n = 195), 57 (63%, n = 169), and 2 (42%, n = 113). Only 2% (n = 6) of the lesions was HPV negative. The highest median viral loads were observed with HPV27 and 57 (i.e. 6.29E+04 and 7.47E+01 viral copies per cell respectively). The multivariate analysis found significant associations between wart persistence and certain wart types, the number of warts, and HPV genotypes. Based on these findings, persistent warts are more likely to: (1) be verruca vulgaris, verruca plantaris simple or mosaic, (2) to manifest as multiple warts, (3) and to be negative for HPV type 2 or 4. These characteristics can be useful in the clinical setting for future risk stratification when considering treatment triage and management.Trial registration: NCT05862441, 17/05/2023 (retrospectively registered).
Decorated graphene oxide with gold nanoparticles as a sensitive modified carbon paste electrode for simultaneous determination of tyrosine and uric acid
Authors: Garazhian Elahe , Kalate Bojdi Majid , Behbahani Mohammad 
Published: October 2023

AbstractIt is presented here as a simple, selective, rapid, low-cost, with a wide linear range method to simultaneously determine tyrosine and uric acid using a modified carbon paste electrode decorated with graphene oxide and gold nanoparticles (GO/AuNPs/MCPE). In order to characterize and evaluate the morphology and constituents of the nanostructures, X-ray diffraction spectroscopy, Transmission electron microscopes, Dynamic light scattering, Zeta potential, electrochemical impedance spectroscopy, and Voltammetry were employed. The current response on the surface of the modified electrode had a dynamic linear range relationship in the concentrations of 0.14–340.00 µmol L−1 and 0.06–141.00 µmol L−1 for tyrosine and uric acid, respectively, and the method detection limit (MDL) was 0.0060 µmol L−1 and 0.0037 µmol L−1, respectively. This modified electrode provided high stability, sensitivity, and acceptable reproducibility for voltammetric measurements of tyrosine and uric acid simultaneously in a biological matrix.
Synthesis and characterization of Co-MOF@Ag2O nanocomposite and its application as a nano-organic catalyst for one-pot synthesis of pyrazolopyranopyrimidines
Authors: Hootifard Ghader , Sheikhhosseini Enayatollah , Ahmadi Sayed Ali , Yahyazadehfar Mahdieh 
Published: October 2023

AbstractIn this study, a Co-MOF was synthesized via a co-precipitation procedure and then used as support for stabilizing Ag ions and producing Co-MOF@Ag2O nanocomposite by microwave irradiation. The characterization of synthesized Co-MOF@Ag2O nanocomposite was performed by using different techniques such as field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) analyses, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) and Fourier-transform infrared (FT-IR). The prepared Co-MOF@Ag2O nanocomposite was applied as a heterogeneous nano-catalyst in the synthesis of pyrazolopyranopyrimidines in water at 50 °C via the one-pot multicomponent reaction of ethyl acetoacetate, hydrazine hydrate, aromatic aldehydes and barbituric acid derivatives. Through this straightforward and effective protocol, different tricyclic fused pyrazolopyranopyrimidines were synthesized at high yields, and short reaction times, through an uncomplicated work-up process with no by-product. The Co-MOF@Ag2O nanocomposite has been effectively recycled for four consecutive cycles without appreciable loss in its activity. Cost-effectiveness, no need for column chromatography, mild conditions, catalyst recyclability, and eco-friendly nature make it a promising candidate compared to other methods.
Reverse pH-dependent fluorescence protein visualizes pattern of interfacial proton dynamics during hydrogen evolution reaction
Authors: Farha Trisha Diba , Kim Samyoung , Imayasu Mieko , Miyawaki Atsushi , Tsutsui Hidekazu 
Published: October 2023

AbstractReverse pH-dependent fluorescent protein, including dKeima, is a type of fluorescent protein in which the chromophore protonation state depends inversely on external pH. The dependence is maintained even when immobilized at the metal-solution interface. But, interestingly, its responses to the hydrogen evolution reaction (HER) at the interface are not reversed: HER rises the pH of the solution around the cathode, but, highly active HER induces chromophore deprotonation regardless of the reverse pH dependence, reflecting an interface-specific deprotonation effect by HER. Here, we exploit this phenomenon to perform scanning-less, real-time visualization of interfacial proton dynamics during HER at a wide field of view. By using dKeima, the HER-driven deprotonation effect was well discriminated from the solution pH effect. In the electrodes of composite structures with a catalyst, dKeima visualized keen dependence of the proton depletion pattern on the electrode configuration. In addition, propagations of optical signals were observed, which seemingly reflect long-range proton hopping confined to the metal-solution interface. Thus, reverse pH-dependent fluorescent proteins provide a unique tool for spatiotemporal analysis of interfacial proton dynamics, which is expected to contribute to a better understanding of the HER process and ultimately to the safe and efficient production of molecular hydrogen.
Regulation of dermal fibroblasts by human neutrophil peptides
Authors: Niwetbowornchai Nattarika , Chaisirirat Thanawat , Sriswasdi Sira , Saithong Supichcha , Filbertine Grace , Wright Helen L. , Edwards Steven W. , Virakul Sita , Chiewchengchol Direkrit 
Published: October 2023

AbstractHuman neutrophil peptides (HNPs) can induce cell proliferation and activation so their growth promoting activities may have potential clinical benefit. This study investigated the effects of HNPs on human dermal fibroblasts. Differential gene expression in HNP-treated cells and genes involved in regulating intracellular pathways were explored. Dermal fibroblasts were isolated from healthy neonatal foreskin and treated with HNPs in 2D and 3D cell culture systems. The expression of cell proliferation (Ki-67) gene and cell activation (COL1A1) gene plus their proteins was measured. Differential gene expression was determined using RNA-seq, and upregulated and downregulated genes were mapped onto intracellular pathways by KEGG analysis and Gene Ontology databases. HNPs significantly increased cell proliferation without cytotoxicity whilst HNP1 enhanced expression of COL1A1 and type I collagen production in 2D cells and 3D spheroids. RNA-sequencing analysis showed gene clustering with clear separation between HNP1-treated and control groups. A heatmap of top 50 differentially expressed genes was consistent among HNP1-treated samples. Most upregulated genes were associated with cell proliferation and activation as mapped into intracellular pathways whilst most downregulated genes belonged to steroid/arachidonic acid metabolism and inflammatory signaling pathways. HNP1 increased cell proliferation and activation but reduced lipid metabolism and inflammation.
A cross‐cross‐correlation based method for joint coregistration of rotated multitemporal synthetic aperture radar images
Authors: Garcia Laura Parra , Pallotta Luca , Clemente Carmine , Giunta Gaetano , Soraghan John J. 
Published: October 2023

AbstractCoregistration is among the most important and challenging tasks when dealing with multiple synthetic aperture radar (SAR) images, especially when they are acquired at different time instants and characterised by low signal to noise power ratio (SNR) that contributes to their coherence reduction. However, even if some technological expedients could be implemented to maintain the same trajectory and to compensate for these inaccuracies during the acquisition campaign, multitemporal SAR images always need additional registration refinements after compression. Usually, to coregister a series of multitemporal SAR images, one of them is selected as the master, and the remainders are separately registered to it. Differently, in this study, a new strategy is developed to jointly coregister a stack of multitemporal SAR images. It is based on the exploitation of the cross‐correlations in turn computed from each couple of cross‐correlations (a.k.a. cross‐cross‐correlations) of the extracted patches. By doing so, the method is capable of exploiting also the respective misregistration information between the slaves during the estimation process. In this respect, this methodology is applied to enhance the registration capabilities of the constrained Least Squares (CLS) optimisation method, which instead does not account for the reciprocal information related to the slaves. Several tests are performed on multitemporal airborne‐measured SAR data. Obtained results show the effectiveness of the proposed algorithm in terms of achieved root mean square error for images affected by respective rotations also in comparison with the CLS counterpart.
Automated pavement crack detection based on multiscale fully convolutional network
Authors: Wang Xin , Wang Yueming , Yu Lingjun , Li Qi 
Published: October 2023

AbstractAutomatic pavement crack detection is essential for fast and efficient pavement maintenance and health measurement. And crack image data is the basis of crack detection. The existing data collection methods have disadvantages such as high cost, easy loss of frames, blurring, and loss of crack information. Therefore, a new method of data collection using target detection and perspective transformation is introduced. The CRACK2000 dataset with multiple complex backgrounds is constructed by this method. Also, a multiscale fully convolutional network by improving U‐Net, named U‐multiscale dilated network (U‐MDN), is proposed. The network uses U‐Net as the base network and introduces U‐multiscale dilated convolutional module (U‐MDM) after U‐Net downsampling. In addition, the U‐MDM is compared with U‐MCM and MDM, and the result shows that U‐MDM has a better effect. Finally, U‐MDN is compared with U‐Net, CrackSeg, DeeplabV3+, Basnet, and PDDF‐Net on CRACK2000 and other data sets, respectively. The experimental results demonstrate that the U‐MDN is better than other algorithms in terms of precision, recall, F1‐score, and AUC.
Electron paramagnetic resonance of VN–VGa complex in BGaN
Authors: Kierdaszuk J. , Możdżynska E. B. , Drabińska A. , Wysmolek A. , Baranowski J. M. 
Published: October 2023

A metastable photoinduced electron paramagnetic resonance (EPR) signal at low temperatures is reported for GaN alloyed with boron (i.e., BxGa1−xN) epitaxial layers grown at temperatures ranging from 840 to 1090° C. An isotropic EPR line with g = 2.004 is observed with an intensity depending on the growth temperature for all the samples with boron content between 0.73% and 2.51%. The temperature dependence of EPR intensities is compared with the results of high-resolution photoinduced transient spectroscopy. This enables a link between particular traps and the EPR signal. The activation energies of these traps are consistent with the theoretical position of the VN–VGa complex. Thermal annihilation of the EPR signal with 30 meV activation energy corresponds to shallow donor ionization. A model explaining the light-induced EPR signal that involves redistribution of electrons between deep and shallow donors, mediated by photoionization to the conduction band, is proposed.
Enhancing patient-centred care in dentistry: a narrative review
Authors: Böhme Kristensen Camilla , Asimakopoulou Koula , Scambler Sasha 
Published: October 2023

Abstract

Introduction
Patient-centred care (PCC) is widely used within the medical setting, but there is a need for more research on PCC implementation in dentistry.

Sources of data
A narrative review was conducted with literature identified from the Ovid Interface, including several databases such as Embase and Medline.

Areas of agreement
PCC is associated with better health outcomes for patients, and greater work satisfaction among healthcare professionals.

Areas of controversy
Efforts to implement PCC in dentistry are lacking due to several issues including non-consensus about PCC definition and lack of explicit guidelines on how to implement PCC in dentistry.

Growing points and areas timely for developing research
A theory-derived model of PCC explicitly designed for the dental setting was identified. This serves as a starting point to enhance PCC in dentistry, though further research is needed to empirically test the implementation of this model.
Vitamin K supplementation impact in dialysis patients: a systematic review and meta-analysis of randomized trials
Authors: Andrian Titus , Stefan Anca , Nistor Ionut , Covic Adrian 
Published: October 2023

ABSTRACTVitamin K supplementation has been considered recently as a potential treatment for addressing vascular calcification in chronic kidney disease patients.We conducted a systematic review and meta-analysis to summarize the impact of vitamin K supplementation in dialysis patients. Electronic databases were searched for clinical randomized trials among patients treated with vitamin K. Random effects models were performed and risk of bias was evaluated with Cochrane tools and the search was conducted until 15 of September 2023.Eleven trials comprising 830 patients (both adult and pediatric, mainly hemodialysis) compared vitamin K with different controls: lower doses of vitamin K, standard care or placebo. Vitamin K supplementation had no effect on mortality. Vitamin K administration improved vitamin K levels and led to lower levels of dp-uc-MGP and moderately increased calcium levels [0.18 (0.04–0.32)]. Vitamin K1 proved more potency in reducing dp-uc-MGP [SMD −1.64 (−2.05, −1.23) vs. −0.56 (−0.82, −0.31)] and also raised serum vitamin K levels in comparison with vitamin K2 [5.69 (3.43, 7.94) vs. 2.25 (−2.36, 6.87)]. While it did not have a proved benefit in changing calcification scores [−0.14 (−0.37 ± 0.09)], vitamin K proved to be a safe product. There was some concern with bias.Vitamin K supplementation has no impact on mortality and did not show significant benefit in reversing calcification scores. Vitamin K1 improved vitamin K deposits and lowered dp-uc-MGP, which is a calcification biomarker more than vitamin K2. As it proved to be a safe product, additional randomized well-powered studies with improved treatment regimens are needed to establish the true impact of vitamin K in dialysis patients.
Induced polarization of volcanic rocks. Part 7. Kimberlites
Authors: Titov K , Abramov V , Emelianov V , Revil A 
Published: October 2023

SUMMARY
In the field, kimberlites are characterized by high electrical conductivities (about 0.1 S m−1) compared to most igneous rocks. The reason for these high conductivities has not been fully elucidated to date. We investigate here the spectral induced polarization of seven core samples of kimberlites in the frequency range 1.43 mHz–20 kHz. The measurements are made at pore water conductivities ranging from 0.07 to 2.4 S m−1 (NaCl, 25 °C). We also measured the cation exchange capacity (CEC), the specific surface area (SSA) and the magnetic susceptibility of the core samples. We characterized the samples by optical microscopy as well as the X-ray diffraction and thermogravimetric analyses. Based on the electrical measurements, we obtained values of the surface conductivity produced by the double electrical layer coating the solid particles, and the normalized chargeability values characterizing the polarization magnitude of these materials. Mineralogical analyses show significant amount of magnetite (from 2 to 9 wt. per cent, approximately 1 to 4 per cent in vol. content) and smectite (from 1 to 44 wt. per cent) in the core samples. The main contributor of the CEC is smectite because of its very high CEC. The quadrature conductivity, the normalized chargeability, and the surface conductivity are controlled by the CEC normalized by the tortuosity of the pore space (product of the formation factor by the porosity). Our data demonstrate that the conduction and polarization of kimberlites are both controlled by the presence of smectite rather than associated with magnetite. Comparing the new data set and data recently obtained with volcanic rocks from both shield and strato-volcanoes in the previous papers of this series, we show that the model of polarization of the dynamic Stern layer correctly describes the complex electrical conductivity of kimberlites as well. Our results also explain the cause of electrical conductivity anomalies detected at kimberlite pipes and offer new perspectives in using induced polarization method for the exploration of kimberlites around the world.