bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution patterns of human papillomavirus genotypes among women in Guangzhou, China
Authors: Li Shu , Zhang Kelan , Yang Liu , Wu Jia , Bhargava Neha , Li Yinghua , Gao Fei 
Published: October 2023

Abstract
Background
Cervical cancer is associated with high‐risk human papillomavirus (HR-HPV) infection in the world. We aimed to evaluate the status of HPV infection among women in Guangzhou, China.

Methods
The study recruited 28,643 female patients from the Guangzhou Women and Children’s Medical Center for HPV genotype testing between 2019 and 2021.

Results
5668 patients were infected with HPV, resulting in an overall infection prevalence of 19.78%. The prevalence of HR-HPV was recorded at 13.94% (both single-infections and multi-infections), probably high-risk HPV/possibly carcinogenic (pHR-HPV) as 3.51%; and low-risk HPV (LR-HPV) as 3.56%. The most common HR-HPV genotype detected was HPV-52 with an infection rate of 4.99%, followed by HPV 58 (2.18%), 16 (2.12%), 51 (1.61%), 39 (1.19%), 56 (1.09%), 59 (0.85%), 18 (0.72%), 33 (0.61%), 31 (0.53%), 35 (0.20%), 45 (0.17%). Among LR-HPV genotypes, HPV-42 was the most common (1.08%), followed by 44 (0.77%), 81 (0.68%), 6 (0.48%), 43 (0.40%), 11 (0.23%) and 83 (0.07%). The prevalence of infection among different genotypes in pHR-HPV was: 68 (1.29%), 53 (1.21%), 66 (0.77%), 82 (0.25%), 73 (0.16%). Additionally, the prevalence of single genotype HPV infection exceeded that of multiple HPV infections except HPV-59.

Conclusion
Our findings imply that HPV genotype infections in Guangzhou demonstrate a regional and age-related distribution. Therefore, these data can provide a substantial foundation for further epidemiologic analysis to control and prevent HPV infections in Guangzhou.
Dual-drug (Curcumin/Ciprofloxacin) loading and release from chitosan-based hydrogels embedded with magnetic Montmorillonite/Hyaluronic acid for enhancing wound healing
Authors: Sayyar Zahra , Mahdavinia Gholam Reza , Khataee Alireza 
Published: October 2023

AbstractMontmorillonite (MMt) is extensively applied as an efficient drug-carrier in designing drug delivery systems (DDS) due to its high specific surface area to load drugs. Modification of MMt via iron (Fe) blending can thus be a desirable method to improve its biocompatibility. Herein, magnetic nano-carriers involving the magnetic MMt (mMMt) core surrounded by chitosan (Chito) as a biopolymer and hyaluronic acid (HA) were prepared. To coat the mMMt fabricated through the coprecipitation of the Fe3+/Fe2+ ions in the presence of MMt, the acquired mMMt as the core was then treated with the Chito/HA solution to induce the cross-linked Chito/HA as the shell (namely, the Chito/HA-mMMt). The transmission electron microscopy (TEM) results accordingly revealed the existence of the mMMt inside the Chito/HA solution. Curcumin (CUR) and ciprofloxacin (CIP) were further employed as two model drugs. The CUR and CIP release from the Chito/HA-mMMt subsequently occurred in a sustained manner and pH-dependently. Additionally, an upsurge in the CUR and CIP release by applying an external magnetic field was observed. Thus, the prepared Chito/HA-mMMt hydrogels promise an outstanding potential performance in terms of expanding novel pH-dependent DDS with a sustained release behavior. The scratch assay of the given hydrogels also confirms their applications for wound healing.
Extracorporeal cardiopulmonary resuscitation for hypothermic refractory cardiac arrests in urban areas with temperate climates
Authors: Soumagnac Tal , Raphalen Jean-Herlé , Bougouin Wulfran , Vimpere Damien , Ammar Hatem , Yahiaoui Samraa , Dagron Christelle , An Kim , Mungur Akshay , Carli Pierre , Hutin Alice , Lamhaut Lionel 
Published: October 2023

Abstract
Background
Accidental hypothermia designates an unintentional drop in body temperature below 35 °C. There is a major risk of ventricular fibrillation below 28 °C and cardiac arrest is almost inevitable below 24 °C. In such cases, conventional cardiopulmonary resuscitation is often inefficient. In urban areas with temperate climates, characterized by mild year-round temperatures, the outcome of patients with refractory hypothermic out-of-hospital cardiac arrest (OHCA) treated with extracorporeal cardiopulmonary resuscitation (ECPR) remains uncertain.

Methods
We conducted a retrospective monocentric observational study involving patients admitted to a university hospital in Paris, France. We reviewed patients admitted between January 1, 2011 and April 30, 2022. The primary outcome was survival at 28 days with good neurological outcomes, defined as Cerebral Performance Category 1 or 2. We performed a subgroup analysis distinguishing hypothermic refractory OHCA as either asphyxic or non-asphyxic.

Results
A total of 36 patients were analysed, 15 of whom (42%) survived at 28 days, including 13 (36%) with good neurological outcomes. Within the asphyxic subgroup, only 1 (10%) patient survived at 28 days, with poor neurological outcomes. A low-flow time of less than 60 min was not significantly associated with good neurological outcomes (P = 0.25). Prehospital ECPR demonstrated no statistically significant difference in terms of survival with good neurological outcomes compared with inhospital ECPR (P = 0.55). Among patients treated with inhospital ECPR, the HOPE score predicted a 30% survival rate and the observed survival was 6/19 (32%).

Conclusion
Hypothermic refractory OHCA occurred even in urban areas with temperate climates, and survival with good neurological outcomes at 28 days stood at 36% for all patients treated with ECPR. We found no survivors with good neurological outcomes at 28 days in submersed patients.
Head injury in older adults presenting to the ambulance service: who do we convey to the emergency department, and what clinical variables are associated with an intracranial bleed? A retrospective case–control study
Authors: Barrett J. W. , Williams J. , Skene S. S. , Griggs J. E. , Bootland D. , Leung J. , Da Costa A. , Ballantyne K. , Davies R. , Lyon R. M. 
Published: October 2023

Abstract
Objective
Most older adults with traumatic brain injuries (TBI) reach the emergency department via the ambulance service. Older adults, often with mild TBI symptoms, risk being under-triaged and facing poor outcomes. This study aimed to identify whether sufficient information is available on the scene to an ambulance clinician to identify an older adult at risk of an intracranial haemorrhage following a head injury.

Methods
This was a retrospective case–control observational study involving one regional ambulance service in the UK and eight emergency departments. 3545 patients aged 60 years and over presented to one regional ambulance service with a head injury between the 1st of January 2020 and the 31st of December 2020. The primary outcome was an acute intracranial haemorrhage on head computed tomography (CT) scan in patients conveyed to the emergency department (ED). A secondary outcome was factors associated with conveyance to the ED by the ambulance clinician.

Results
In 2020, 2111 patients were conveyed to the ED and 162 patients were found to have an intracranial haemorrhage on their head CT scan. Falls from more than 2 m (adjusted odds ratio (aOR) 3.45, 95% CI 1.78–6.40), chronic kidney disease (CKD) (aOR 2.80, 95% CI 1.25–5.75) and Clopidogrel (aOR 1.98, 95% CI 1.04–3.59) were associated with an intracranial haemorrhage. Conveyance to the ED was associated with patients taking anticoagulant and antiplatelet medication or a visible head injury or head injury symptoms.

Conclusion
This study highlights that while most older adults with a head injury are conveyed to the ED, only a minority will have an intracranial haemorrhage following their head injury. While mechanisms of injury such as falls from more than 2 m remain a predictor, this work highlights that Clopidogrel and CKD are also associated with an increased odds of tICH in older adults following a head injury. These findings may warrant a review of current ambulance head injury guidelines.
Redefining ARDS: a paradigm shift
Authors: Villar Jesús , Szakmany Tamas , Grasselli Giacomo , Camporota Luigi 
Published: October 2023

AbstractAlthough the defining elements of “acute respiratory distress syndrome” (ARDS) have been known for over a century, the syndrome was first described in 1967. Since then, despite several revisions of its conceptual definition, it remains a matter of debate whether ARDS is a discrete nosological entity. After almost 60 years, it is appropriate to examine how critical care has modeled this fascinating syndrome and affected patient’s outcome. Given that the diagnostic criteria of ARDS (e.g., increased pulmonary vascular permeability and diffuse alveolar damage) are difficult to ascertain in clinical practice, we believe that a step forward would be to standardize the assessment of pulmonary and extrapulmonary involvement in ARDS to ensure that each patient can receive the most appropriate and effective treatment. The selection of treatments based on arbitrary ranges of PaO2/FiO2 lacks sufficient sensitivity to individualize patient care.
Human cytomegalovirus seropositivity is associated with reduced patient survival during sepsis
Authors: Unterberg M. , Ehrentraut S. F. , Bracht T. , Wolf A. , Haberl H. , von Busch A. , Rump K. , Ziehe D. , Bazzi M. , Thon P. , Sitek B. , Marcus K. , Bayer M. , Schork K. , Eisenacher M. , Ellger B. , Oswald D. , Wappler F. , Defosse J. , Henzler D. , Köhler T. , Zarbock A. , Putensen C. P. , Schewe J. C. , Frey U. H. , Anft M. , Babel N. , Steinmann E. , Brüggemann Y. , Trilling M. , Schlüter A. , Nowak H. , Adamzik M. , Rahmel T. , Koos B. ,   SepsisDataNet.NRW research group
Published: October 2023

Abstract
Background
Sepsis is one of the leading causes of death. Treatment attempts targeting the immune response regularly fail in clinical trials. As HCMV latency can modulate the immune response and changes the immune cell composition, we hypothesized that HCMV serostatus affects mortality in sepsis patients.

Methods
We determined the HCMV serostatus (i.e., latency) of 410 prospectively enrolled patients of the multicenter SepsisDataNet.NRW study. Patients were recruited according to the SEPSIS-3 criteria and clinical data were recorded in an observational approach. We quantified 13 cytokines at Days 1, 4, and 8 after enrollment. Proteomics data were analyzed from the plasma samples of 171 patients.

Results
The 30-day mortality was higher in HCMV-seropositive patients than in seronegative sepsis patients (38% vs. 25%, respectively; p = 0.008; HR, 1.656; 95% CI 1.135–2.417). This effect was observed independent of age (p = 0.010; HR, 1.673; 95% CI 1.131–2.477). The predictive value on the outcome of the increased concentrations of IL-6 was present only in the seropositive cohort (30-day mortality, 63% vs. 24%; HR 3.250; 95% CI 2.075–5.090; p < 0.001) with no significant differences in serum concentrations of IL-6 between the two groups. Procalcitonin and IL-10 exhibited the same behavior and were predictive of the outcome only in HCMV-seropositive patients.

Conclusion
We suggest that the predictive value of inflammation-associated biomarkers should be re-evaluated with regard to the HCMV serostatus. Targeting HCMV latency might open a new approach to selecting suitable patients for individualized treatment in sepsis.
Trends and inequity in improved sanitation facility utilisation in Bangladesh: Evidence from Bangladesh Demographic and Health Surveys
Authors: Ahmed Anisuddin , Sayeed Abu , Tanwi Tania Sultana , Saha Nondo , Hanson Molly , Protyai Dipanjan Adhikary , Hossain Aniqa Tasnim , Ahmed Ali , Rahman Fariya , Akter Ema , Nusrat Nowrin , Badsha Md Shawon , Rahman Afruna , Islam Md Khairul , Alam Md. Shah , Nahar Quamrun , Arifeen Shams El , Rahman Ahmed Ehsanur , Tahsina Tazeen 
Published: October 2023

AbstractImproved sanitation is indispensable to human health. However, lack of access to improved sanitation remains one of the most daunting public health challenges of the twenty-first century in Bangladesh. The aim of the study was to describe the trends in access to improved sanitation facilities following the inequity gap among households in different socioeconomic groups in Bangladesh. Data from the Bangladesh Demographic and Health Survey (BDHS) 2007, 2011, 2014, and 2017-18 were extracted for this study. Inequity in access to improved sanitation was calculated using rich-poor ratio and concentration index to determine the changes in inequity across the time period. In Bangladesh, the proportion of households with access to improved sanitation increased steadily from 25.4% to 45.4% between 2007 and 2014, but slightly decreased to 44.0% in 2017-18. Age, educational status, marital status of household head, household wealth index, household size, place of residence, division, and survey year were significantly associated with the utilisation of improved sanitation. There is a pro-rich situation, which means that utilisation of improved sanitation was more concentrated among the rich across all survey years (Concentration Index ranges: 0.40 to 0.27). The government and other relevant stakeholders should take initiatives considering inequity among different socioeconomic groups to ensure the use of improved sanitation facilities for all, hence achieving universal health coverage.
The association of Treg and Th17 cells development factors and anti-TPO autoantibodies in patients with recurrent pregnancy loss
Authors: Niafar Mitra , Samaie Vajihe , Soltani-Zangbar Mohammad Sadegh , Motavalli Roza , Dolati Sanam , Danaii Shahla , Mehdizadeh Amir , Yousefi Mehdi 
Published: October 2023

Abstract
Objectives
Thyroid autoimmunity is considered as the most prevalent autoimmune condition in women in fertility age. There are different clinical evidences indicating the association between thyroid autoimmunity and increased risk of RPL. This study aimed to analyze the association of Tregs and Th17 cells development factors and anti–thyroid peroxidase (anti-TPO) antibodies in RPL patients. Healthy controls (n = 36), TPO + controls (n = 25) and TPO + RPL (n = 32) participated in this study. After blood sampling, the frequency of Th17 and Tregs was evaluated using flow cytometry. Real-time PCR and ELISA was used to assess the status of Tregs and Th17 related transcription factors and cytokines in mRNA and protein level, respectively.

Results
TPO + RPL group showed a higher Th17 frequency compared to healthy controls and TPO + controls groups (p = 0.0002 and p = 0.04, respectively). Additionally, mRNA expression levels of RORγT and IL-17 were significantly higher in TPO + RPL compared to healthy controls and TPO + controls groups. In contrast, Foxp3 and TGFβ expression was lower in TPO + RPL. ELISA findings also indicated a significantly higher IL-17 and lower TGFβ secretion in TPO + RPL compared to healthy controls and TPO + controls. Thyroid autoimmunity should intensely be controlled specially in patients with RPL history.
Multimodal treatment, including extracorporeal shock wave therapy, for refractory chronic tension-type headache: a case report
Authors: Takekawa Toru , Chino Toshifumi , Yamada Naoki , Watanabe Shu , Abo Masahiro , Sengoku Renpei 
Published: October 2023

Abstract
Background
Few reports have described multidisciplinary treatment, including extracorporeal shock wave therapy, for patients with refractory chronic tension-type headache. In this study, we conducted multidisciplinary treatment for a patient with chronic tension-type headache who suffered from chronic headache refractory to treatment.

Case presentation
The patient was a 45-year-old Japanese male suffering from 20 years of headache. As his headache had worsened recently, he visited a local clinic. With the diagnosis of suspected tension-type headache, its treatment was unsuccessful and he was referred to our hospital. The neurology department confirmed the tension-type headache and prescribed another medication, but he showed no improvement. Then, the patient was referred to the rehabilitation medicine department for consultation. At the initial visit, we identified multiple myofascial trigger points in his bilateral posterior neck and upper back regions. At the initial visit, he was prescribed 10 mL of 1% lidocaine injected into the muscles in these areas. In addition, he received 2000 extracorporeal shock wave therapy into bilateral trapezius muscles, and was instructed to take oral Kakkonto extract granules, benfotiamine, pyridoxine hydrochloride, and cyanocobalamin. Cervical muscle and shoulder girdle stretches and exercises were also recommended. At follow-up treatment visits, we used extracorporeal shock wave therapy to bilateral trapezius muscles, which led to immediate pain relief. After 11 weeks, he was not taking any medication and his headache was subjectively improved and his medical treatment ended.

Conclusion
A patient with chronic tension-type headache refractory to regular treatment was successfully treated with a multimodal approach including extracorporeal shock wave therapy in addition to standard treatment. For patients with tension-type headache accompanied by myofascial trigger points, it may be recommended to promptly consider aggressive multimodal treatment that includes extracorporeal shock wave therapy.
Trauma-informed prevention programmes for depression, anxiety, and substance use among young people: protocol for a mixed-methods systematic review
Authors: Bailey S. , Newton N. , Perry Y. , Grummitt L. , Baams L. , Barrett E. 
Published: October 2023

Abstract
Background
Mental ill-health and substance use bear a substantial burden and harm on young people and often arise from co-occurring and compounding risk factors, such as traumatic stress. Trauma-informed prevention of mental ill-health and substance use demonstrates significant promise in reducing this burden. A systematic literature review is required to identify and summarise the effectiveness, feasibility, acceptability, and design principles underpinning existing trauma-informed mental ill-health and/or substance use prevention programmes for young people aged 12–24 years.

Methods
MEDLINE, Embase, CINAHL, PsychINFO, and Cochrane Library will be searched from 2012 through September 2022. Reference lists of included articles will be citation-chained. Title and abstracts will be screened and two reviewers will review articles full-text. One reviewer will extract data from eligible articles using a piloted data extraction form, and 20% of the data will be verified by a second reviewer. Risk of bias will be assessed using the Cochrane risk-of-bias tool for randomised trials (RoB 2), Risk of Bias in Non-randomised Studies of Interventions (ROBINS-I), and The Joanna Briggs Institute Critical Appraisal Checklist for Quasi-Experimental Studies and The Joanna Briggs Institute Critical Appraisal Checklist for Qualitative Research (CASP), depending on the study type. Characteristics of existing trauma-informed mental ill-health and/or substance use prevention programmes for young people will be summarised narratively. Effectiveness, feasibility, and acceptability will be qualitatively described and summarised, with proportions and effect sizes quantitatively synthesised, where possible.

Discussion
Trauma-informed approaches to prevention demonstrate significant promise, yet to date, no study has systematically summarised and synthesised the available literature. To fill this gap, the present review will systematically identify and summarise the effectiveness, feasibility, acceptability, and design principles underpinning existing trauma-informed mental health and/or substance use prevention programmes for young people aged 12–24. This review will inform the development, adaptation, evaluation, and implementation of future trauma-informed mental ill-health and substance use prevention programmes for young people. Findings will inform critical efforts to interrupt and prevent already elevated trajectories of mental ill-health, substance use, and related harms among those young people exposed to adversity.

Systematic review registration
PROSPERO CRD42022353883.
The effectiveness of past wildfire at limiting reburning is short-lived in a Mediterranean humid climate
Authors: Davim David A. , Rossa Carlos G. , Pereira José M. C. , Guiomar Nuno , Fernandes Paulo M. 
Published: October 2023

Abstract
Background
The study of wildfire interactions (i.e., spread limitation and reburns) is gaining traction as a means of describing the self-limiting process of fire spread in the landscape and has important management implications but has scarcely been attempted in Europe. We examined to what extent previously burned areas restricted the development of individual large wildfires (> 500 ha) in mainland Portugal.

Results
For the 1984–2021 period, we (1) modeled the proportion of large wildfire perimeters coinciding with transitions to shorter time since fire (TSF), i.e., locations where fire spread ceased upon encountering assumedly less flammable fuels, and (2) characterized the prevalence of different TSF in the composition of the area burned by large wildfires in relation to available TSF. Only 4% of the large wildfires did not comprise edges intersecting past wildfires. Low TSF (especially up to 8 years) resulted in large-wildfire perimeter limitation at TSF transitions. This effect was further enhanced by high historical burn probability and proximity to roadways and watercourses. Perimeter limitation did also increase under high (but not very high or extreme) fire danger, benefiting from maximum seasonal firefighting preparedness. TSF prevalence in the composition of large-wildfire area was extremely variable and thus an overall weak pattern emerged, with minimum and maximum prevalence respectively at TSF < 2 years and TSF ≥ 6 years.

Conclusions
Large wildfire limitation in Portugal is hampered by fast fuel build-up after fire, indicating a short-lived fire-hazard reduction effect under the prevailing Mediterranean humid climate of the study region. Nonetheless, such effect should be considered when planning fuel-reduction treatments and can be used opportunistically during large-wildfire suppression operations.