bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of an enhanced medication access workflow within a health-system specialty pharmacy: Impact on patient and clinician experience
Authors: Harkonen Brandon , Cuttitta Anthony , Henderson James , Gavrila Valerie , Mckibbin Jey , Luttman Sherrie , Benedict Wendy , Kelley Lindsey R , Flanders Scott A , Choe Hae Mi , Barnes Geoffrey D 
Published: February 2025

Abstract

Disclaimer
In an effort to expedite the publication of articles, AJHP is posting manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time.

Purpose
The initiation of specialty medications is associated with patient access challenges and clinician burden. This evaluation assessed the impact on patient and clinician experience of an intervention to improve medication access by investing resources upstream of the prescribing step.

Methods
The specialty pharmacy intervention was designed to improve medication access within 5 medical specialty clinics by utilizing an embedded medication access team assigned to patients and prescribers of targeted medications. Using a multimethods evaluative approach to quantitatively assess associations between the referral process and patient experience, we analyzed the emotional valence of patient portal messages using a retrospective cohort study within the event study framework of a nonrandomized, stepped wedge implementation design. Semistructured qualitative interviews provided an understanding of clinician experience.

Results
The intervention was associated with an increase in the net positive emotional valence of patient portal messages (average marginal effect, 5.3; 95% CI, 3.8-6.8; P &lt; 0.001). Except for patients seen by gastroenterologists for irritable bowel disease, patients cared for in all other specialties experienced statistically significant increases in net positive valence in the primary analysis. Regarding clinician experience, 4 major interrelated themes emerged from 17 qualitative interviews with prescribers and pharmacists: (1) decreased clinician burden, general praise, (2) improved experience and satisfaction, reduced anxiety and concerns, (3) rewarding praise for other prescribers/colleagues, and (4) excellent coordination, efficiency, and speed.

Conclusion
Investing staff resources before, during, and after the prior authorization process greatly improved clinician experience. The positive valence of patient portal messages also increased, suggesting patient experience improvements.
Evaluating the light environment as a contributor to colour differences among related bird species
Authors: Fialko Kristina , Price Trevor D 
Published: February 2025

Abstract
Understanding the diversity of colour in nature has been one of the more elusive evolutionary problems. In the terrestrial environment, comparative analyses have associated differences in colour between species to light environment, background, and receiver perception. However, these account for only a small fraction of colour diversity, and it has been difficult to explain why a certain species is the colour it is. Here we examine colour variation across 12 very similar species of warblers belonging to the genus Phylloscopus, whose general brightness along an achromatic axis has previously been related to light intensity in their habitat. Many of these species also show variation in the colour of several plumage regions, including the wing bar, belly, and white vs. green in the outer tail feathers. We ask if these differences can be connected to the spectrum of light found in the habitat of each species. We find little evidence that contrast between patches and adjacent plumage or colour per se is affected by light environment. We argue that the heterogeneity of light environments experienced within a habitat and throughout the day make it unlikely that downwelling irradiance alone has a direct influence on colour variation. Accordingly, other features must have driven colour evolution. Diversification may be driven by environmental characteristics, such as background, or unrelated to environment altogether, reflecting the possibility that many different variants may effectively stimulate a receiver, and those that appear in a certain species reflect stochastic processes (e.g. mutation) and contingency (form of the ancestor).
Acute and long-term transcriptomic responses of granulosa cells to obesogenic diet and concomitant effects on oocyte quality: insight from an outbred mouse model
Authors: Marei Waleed F A , Moorkens Kerlijne , Gansemans Yannick , Van Nieuwerburgh Filip , Leroy Jo L M R 
Published: February 2025

Abstract
Diet-induced obesity can cause long-term alterations in ovarian functions, but the acute effects of obesogenic diets on the follicular cells and their progression over time, when intake is continued and obesity develops, remain unclear. We aimed to determine the onset and progression of changes in the granulosa cell transcriptomic profile after starting a high-fat/high sugar (HFHS)-diet feeding in mice. We also examined the changes in oocyte lipid droplet content and mitochondrial ultrastructural abnormalities. Swiss (outbred) mice were sacrificed at 24 h, 3 days, and at 1, 4, 8, 12, and 16 weeks of feeding HFHS and control diets. Lipid droplet content significantly increased in the HFHS oocytes within 24 h compared to controls (P &lt; 0.05). Oocyte mitochondrial abnormalities only increased starting from 8 weeks. Granulosa RNA-seq revealed altered transcriptomic gene-set enrichments (GO terms and KEGG pathways, Padj &lt; 0.05) already at 3 days and 1 week indicating acute endoplasmic reticulum unfolded protein responses, with concomitant fluctuations in several cellular metabolic pathways and gene sets related to mitochondrial bioenergetic functions, some of which persisted after 8 weeks. Interestingly, the short- and long-term patterns of changes in cytochrome P450, steroid hormone biosynthesis, retinol metabolism, bile acid metabolism, fatty acid metabolism, and Pi3K/Akt signaling pathways were most prominent and highly correlated; all being acutely upregulated, then chronically downregulated. These results show that the impact of obesogenic diet on the oocyte and granulosa cells is prompt, while the response depends on the duration of feeding and occurs in a multiphasic cascade together with a progressive deterioration in oocyte quality.
Waterbody connectivity: Linking science and policy for improved waterbody protection
Authors: Sulliván S Mažeika Patricio , Hughes Robert M , Vadas Robert L , Davies Gillian T , Shirey Patrick D , Colvin Susan A R , Infante Dana M , Danehy Robert J , Sanchez Nayeli K , Keast Robert B 
Published: February 2025

Abstract
Connectivity—the flows and exchanges of organisms, materials, and energy within and among watersheds—is a central paradigm in ecosystem science. Changes in those connections have consequences for ecosystem functioning and water quality downstream and upstream. Therefore, connectivity is a cornerstone for federal water protection under the United States Clean Water Act (CWA). In the present article, we review the science of connectivity, explain the history of changes in CWA jurisdiction defining waters of the United States, discuss implications for US water policy, and highlight key steps to align that policy with science. The Sackett v. EPA (2023) Supreme Court decision significantly reduced federal water protection for wetlands and headwaters, disregarding their connectivity with larger, protected waterbodies. To fulfill the goals of the CWA to restore and maintain the chemical, physical, and biological integrity of U.S. waters, Congress must amend the act, fully recognizing the multidimensional connectivity of aquatic ecosystems and ultimately incorporating these connections into ecosystem-level policy.
Neuronal α-synuclein toxicity is the key driver of neurodegeneration in multiple system atrophy
Authors: Wiseman James A , Halliday Glenda M , Dieriks Birger Victor 
Published: February 2025

Abstract
Multiple system atrophy (MSA) is a rare, rapidly progressing neurodegenerative disorder often misdiagnosed as Parkinson’s disease (PD). While both conditions share some clinical features, MSA is distinct in its pathological hallmark: oligodendroglial cytoplasmic α-synuclein (α-Syn) inclusions, known as glial cytoplasmic inclusions (GCIs). These GCIs are pathognomonic for MSA, but they do not lead to significant oligodendroglial cell loss. Instead, MSA is characterised by a substantially greater loss of non-dopaminergic neurons in the nigrostriatal and olivopontocerebellar systems compared to PD. This widespread neuronal degeneration, which is not seen to the same extent in PD, plays a critical role in MSA’s clinical presentation and is important to consider if PD is to be redefined as a neuronal α-Syn disease. It also raises the question of differences in the potential toxicity of lesions in MSA and the underlying cause of neuronal death in MSA.
By combining an N-terminus α-Syn antibody that reveals more α-Syn pathology and super-resolution microscopy, we identified α-Syn fibrils in MSA neurons penetrating the nucleus from the cytoplasm, leading to nuclear destruction and neuronal death. Our data indicate an early invasion of neuronal nuclei by α-Syn pathology in MSA, precipitating rapid nuclear envelope destruction, as observed through significant structural damage, including the loss of Lamin integrity. Although the progression of α-Syn pathology from the cytoplasm to the nucleus may be similar in oligodendroglia and neurons, the aggregation state of the α-Syn proteoforms involved differs as proteolytic resistance of α-Syn inclusions is significantly higher in neurons and the nucleus is destroyed.
We describe the progressive impact of α-Syn nuclear pathology on MSA neurons and show that this is a more detrimental and rapid pathology driving neurodegeneration. Our data suggest that oligodendroglial inclusions contain more soluble, less toxic α-Syn proteoforms, consistent with two distinct α-Syn filaments in MSA. We propose renaming MSA as a neuronal nuclear and oligodendroglial α-synucleinopathy to better reflect these two distinct pathologies.
Air bubbles around the heart: a rare case report of pneumohydropericardium in heart transplantation
Authors: Brucato Filippo , Gentile Piero , Musca Francesco , Casadei Francesca , Palazzini Matteo 
Published: February 2025

Abstract

Background
Pneumohydropericardium is a rare but significant condition characterized by the presence of both air and fluid in the pericardial space. It is often associated with trauma, invasive procedures, or thoracic pathologies, and its occurrence following heart transplantation is particularly unusual. Early recognition is essential to prevent complications such as cardiac tamponade, although conservative management can be effective in cases without haemodynamic compromise.

Case summary
We present the case of a 40-year-old woman with a history of hypokinetic-dilative cardiomyopathy and heterozygous for TNNT2 gene mutation (c.547C&gt;T p.Arg183Trp), who underwent orthotopic heart transplantation. Following the procedure, she developed pneumohydropericardium, identified by echocardiography as air microbubbles and fluid in the pericardial space, without associated haemodynamic instability. The condition was monitored with serial echocardiograms, and no invasive intervention was required. The air microbubbles resolved after 16 days of echocardiographic follow-up, and the patient was discharged in stable condition. At follow-up, she showed no recurrence of the pneumohydropericardium.

Discussion
This case highlights pneumohydropericardium as a rare post-transplant complication that, when detected early and closely monitored, can be managed conservatively without severe outcomes. While pneumohydropericardium has been previously reported in association with other conditions, its post-transplant manifestation is rare and warrants further awareness. Echocardiographic surveillance played a key role in guiding management, as invasive procedures were avoided, and the patient remained haemodynamically stable throughout. This case underscores the importance of recognizing and managing pneumohydropericardium in heart transplant patients to ensure optimal outcomes
Single lung transplantation is safe when the other lung is declined
Authors: Asija Richa , Fuller Joshua , Costa Joseph , Abramov Alexey , Grewal Harpreet , Benvenuto Luke , Magda Gabriela , Shah Lori , DiMango Angela , Robbins Hilary , Stanifer Bryan Payne , Sonett Joshua , Arcasoy Selim , D’Ovidio Frank , Lemaitre Philippe 
Published: February 2025

Abstract

OBJECTIVES
Single lung transplant (SLT) is an acceptable treatment modality for certain patients with end-stage lung disease. SLT occurs when two appropriate donor lungs are split between recipients (‘split singles’) or when one donor lung is adequate for transplant and the other lung is declined (‘isolated single’). There is a paucity of literature investigating the outcomes in patients who received an isolated SLT. This study analyses the characteristics and survival outcomes of isolated SLT recipients.

METHODS
The transplant database at our institution was queried for all lung transplants between 2010 and 2021. The primary outcome of survival was assessed using Kaplan–Meier curves and Cox regression modelling. Secondary outcomes were assessed using Cox regression and Fisher’s exact test.

RESULTS
Of 759 lung transplant recipients, 164 patients underwent a split SLT, and 271 patients underwent an isolated SLT. There was no significant difference when comparing most demographic characteristics between isolated SLT and split SLT patients. Isolated SLT recipients had similar overall mortality when compared to split SLT recipients (HR 0.97, 95% CI 0.72–1.33, P = 0.87). There was no difference in postoperative need for extracorporeal membrane oxygenation (P = 0.209), duration of postoperative ventilation (P = 0.408) and length of hospitalization (P = 0.443).

CONCLUSIONS
Our analysis demonstrating similar overall survival between recipients of isolated SLT and split SLT shows that a well-selected isolated donor lung can be used safely in the appropriate recipient population. This practice allows expansion of a known scarce donor lung pool and reduction of the waitlist mortality in lung transplant candidates.
Mechanical Circulatory Support in Acute Pulmonary Embolism
Authors: Mentzel Luise , Fengler Karl , Oezkur Mehmet , Lanmüller Pia , Tavazzi Guido , Thevathasan Tharusan , Iannaccone Mario , Werner Nikos 
Published: February 2025

Abstract
Acute pulmonary embolism is a life-threatening condition caused by the thromboembolic obstruction of pulmonary arteries, which often leads to hemodynamic instability, right ventricular failure, and potentially death. Patients with massive or high-risk PE are particularly vulnerable and may not respond to conventional treatments, necessitating the implementation of mechanical circulatory support. Here, we discuss recent advancements in the diagnosis and multidisciplinary management of patients with high-risk PE and highlight the need for rapid and personalized treatment strategies.
The role of temporary mechanical circulatory support in acute on chronic heart failure
Authors: Vandenbriele Christophe , Bertoldi Letizia , Bruno Francesco , Haneya Assad , Nap Alexander , Potapov Evgenij , Schönrath Felix , Zimpfer Daniel , Pappalardo Federico 
Published: February 2025

Abstract
Heart failure remains a leading cause of morbidity and mortality worldwide, with acute heart failure or cardiogenic shock requiring rapid intervention to prevent fatal outcomes. Advances in temporary mechanical circulatory support devices (tMCS) have revolutionized the management of advanced heart failure, offering temporary, durable, and individualized support options. This manuscript reviews the pathophysiology and clinical presentation of acute heart failure, the role of multidisciplinary Heart Teams, and the growing importance of structured care networks in managing complex cases of heart failure. We explore the strategic deployment of tMCS in acute settings, device options, implications for patient outcomes, and current challenges in the field. This manuscript emphasizes the importance of team-based approaches and underscores the potential of tMCS devices in stabilizing patient haemodynamics, bridging to recovery or definitive therapy, and improving survival in patients facing high-risk heart failure.
Sex differences in secondary prevention needs and recommendations among patients with spontaneous coronary artery dissection
Authors: Stragapede Elisa , Bouchard Karen , Coutinho Thais , Mulvagh Sharon , Pacheco Christine , Liu Shuangbo , Saw Jacqueline , So Derek , Reed Jennifer L , Robert Helen , Lappa Nadia , Tulloch Heather 
Published: February 2025

Abstract
Spontaneous coronary artery dissection (SCAD) is increasingly recognized as a cause of heart attacks, particularly for female patients; however, ∼10% of cases occur in male patients. While male and female differences pertaining to clinical outcomes and treatment protocols have been addressed in the literature, research is limited on sex differences in secondary prevention programming, such as cardiac rehabilitation. Cardiac rehabilitation is recommended for patients with SCAD to promote physical and mental recovery. This letter highlights sex-specific gaps in existing programming and reports patients’ suggestions for enhancing the recovery of this unique patient population.
Influenza a virus subtype H9N2 infection induces respiratory microbiota dysbiosis in chickens via type-I interferon-mediated mechanisms
Authors: Oladokun Samson , Alizadeh Mohammadali , Mallick Amirul I , Fazel Fatemeh , Doost Janan Shoja , Blake Katherine , Denis Myles St , Raj Sugandha , Sharif Shayan 
Published: February 2025

Abstract
Avian influenza virus (AIV) poses significant threats to poultry and human health. This study investigates the impact of H9N2 AIV infection on the respiratory microbiota of chickens using 16S rRNA gene sequencing. Total 48 one-day-old specific pathogen-free chickens were assigned to six groups: a control and five post-infection groups (days 1, 3, 5, 7, and 9). After a 15-day microbiota stabilization period, the infected chickens received a viral inoculum (107 TCID50/ml) via ocular, intra-nasal, and intra-tracheal routes. Tracheal and broncho-alveolar lavage samples were analyzed. Significant reductions in microbiota diversity were observed on days 5, 7, and 9 post-infection, compared to d0 controls. Permutational Multivariate Analysis of Variance confirmed significant beta diversity differences (P = 0.001) between infected and uninfected groups. The microbial shifts from d5 to d9 were marked by increased Proteobacteria, decreased Actinobacteria and Firmicutes, and a rise in Dickeya. Elevated type-I interferon (IFN-β) and viperin gene expression at d5 coincided with reduced microbiota diversity, highlighting the respiratory microbiota’s role in modulating host responses to AIV H9N2 infection and suggesting potential biomarkers for respiratory dysbiosis.
The genome sequence and genomic diversity of soybean tentiform leafminer (Macrosaccus morrisella)
Authors: Faulk Christopher , Ribeiro Arthur V , Walls Carrie , Koch Robert L 
Published: February 2025

Abstract
The soybean tentiform leafminer, Macrosaccus morrisella (Fitch) (Lepidoptera: Gracillariidae), is native to North America where it was known to feed on American hogpeanut and slickseed fuzzybean. However, it has recently expanded its host range to include soybean, an important agricultural crop. Here, we report a new, highly contiguous genome for this species with a length of 245 Mb, N50 of 9 Mb, and 96.33% BUSCO completeness. The mitochondrial genome shares only 81% identity to its nearest relative in the NCBI nucleotide database indicating long-standing divergence or sparse sequencing in this clade. To determine whether host plant choice is genetically driven, we sequenced 18 individuals across 3 locations in Minnesota, USA, collected from both American hogpeanut and soybean plants. Genetic variation did not correlate with population structure based on either geography or host plant species (weighted FST estimate: 0.0058). As a secondary measure, we independently assembled complete mitochondrial genomes from all individuals and observed no delineation between host or location. The overall lack of detectable population structure at the nuclear and mitochondrial genome levels suggests a large population with flexible dietary preferences and does not show evidence of genetically driven host preference.
Caregiver assessment of socioemotional functioning in people living with neurodegenerative disease (Part 2): Concurrent validity and test-retest reliability of a new measure
Authors: Brown Casey K , Otero Marcela C , Ascher Elizabeth A , Merrilees Jennifer , Levenson Robert W 
Published: February 2025

Abstract

Background and Objectives
Care recipients with neurodegenerative disease can have socioemotional impairments that are challenging for their caregivers. We examine the concurrent and incremental validity of a novel informant-rated measure of care recipient functioning, the Caregiver Assessment of Socioemotional Functioning (CASEF), by examining links with clinician-rated behavioral problems, neuropsychiatric symptoms, and disease severity, as well as caregivers’ reported burden and relationship quality. We also examine test-retest reliability of the CASEF.

Research Design and Methods
Informal caregivers (N=177) of individuals with neurodegenerative disease reported on their care recipients’ socioemotional functioning and completed structured interviews with clinicians to assess care recipients’ disease severity and neuropsychiatric symptoms. Care recipients’ behavioral symptoms were evaluated with clinician rating scales. Another sample of caregivers (N=61) repeated the CASEF four to six months following an initial administration.

Results
CASEF subscales evidenced concurrent validity with other measures of care recipients’ socioemotional functioning, disease severity, neuropsychiatric symptoms, and behavioral symptoms. CASEF subscales also evidenced incremental validity by accounting for significant additional variance in caregiver burden and relationship quality after accounting for care recipients’ neuropsychiatric and behavioral symptoms. Finally, CASEF subscales demonstrated adequate to excellent test-retest reliability.

Discussion and Implications
Findings highlight the validity and reliability of the CASEF. Coupled with findings from Part 1 of this series (which established CASEF’s construct validity), these findings support using the CASEF in clinical and research settings to assess changes in care recipient socioemotional functioning over time and to examine links between care recipient socioemotional changes and caregiver burden, health, and well-being.