bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiology of Helicobacter pylori Infection
Authors: Kikuchi Shogo , Dore Maria P. 
Published: September 2005

ABSTRACTThis review summarizes epidemiologic studies published between April 2004 and March 2005. DNA of Helicobacter pylori was detected in river water, but the culture was unsuccessful. H. pylori infection was associated with Shigella infection. Despite many studies, predominant infection routes of H. pylori have not yet been clearly identified. In some limited populations in developing countries, H. pylori infection was rare or with strange distributions. Trials to reduce the H. pylori infection rate were performed including H. pylori eradication in total family units and fly control.The hypothesis of a causal role of Helicobacter species and H. pylori infection in cancer of the hepatobiliary tract was indeed confirmed.
Diagnosis of Helicobacter pylori Infection
Authors: Krogfelt Karen A. , Lehours Philippe , Mégraud Francis 
Published: September 2005

ABSTRACTA large number of studies on diagnostic tests have been published this year. New tests were proposed for the detection of Helicobacter pylori antigens in stools and new molecular methods (real‐time polymerase chain reaction) to look for antimicrobial susceptibility. The other standard tests have been applied in different situations to improve the diagnosis of the infection.
Widespread Electroanatomic Alterations of Right Cardiac Chambers in Patients with Myotonic Dystrophy Type 1
Authors: RUSSO ANTONIO DELLO , PELARGONIO GEMMA , PARISI QUINTINO , SANTAMARIA MATTEO , MESSANO LOREDANA , SANNA TOMMASO , CASELLA MICHELA , DE MARTINO GIUSEPPE , DE PONTI ROBERTO , PACE MANUELA , GIGLIO VINCENZO , IERARDI CAROLINA , ZECCHI PAOLO , CREA FILIPPO , BELLOCCI FULVIO 
Published: September 2005

Introduction: Conduction disturbances and arrhythmias characterize the cardiac feature of myotonic dystrophy type 1 (MD1); a myocardial involvement has been suggested as part of the cardiac disease. The aim of our study was to investigate the underlying myocardial alterations using electroanatomic mapping (CARTO) and their possible correlation with genetic and neurological findings. Methods and Results: Right atrial and ventricular CARTO maps were obtained in 13 MD1 patients. Thirteen age‐matched patients with paroxysmal supraventricular tachycardia and normal heart served as controls. Unipolar voltage (UNI‐v), bipolar voltage (BI‐v) amplitudes, bipolar potential duration (Bi‐dur), and atrial propagation time (A‐pt) were measured. UNI‐v and BI‐v in interatrial septum, anterolateral atrial wall, and right ventricle outflow tract were lower in MD1 patients than controls (P < 0.001). Bi‐dur and A‐pt were longer in MD1 patients than controls (P < 0.001, P = 0.046, respectively). A significant relationship was documented between CTG triplets and the percentage of Bi‐v <0.5 mV in the atrial anteroseptal region (r = 0.6, P = 0.02). Conclusions: Altered electroanatomic patterns are present in the right cardiac chambers in MD1 patients. Widespread myocardial alterations, not necessarily limited to the conduction system, may support the presence of a cardiac myopathy as part of the disease.
Flutter Localized to the Anterior Left Atrium After Catheter Ablation of Atrial Fibrillation
Authors: JAÏS PIERRE , SANDERS PRASHANTHAN , HSU LI‐FERN , HOCINI MÉLÈZE , SACHER FREDERIC , TAKAHASHI YOSHIHIDE , ROTTER MARTIN , ROSTOCK THOMAS , BORDACHAR PIERRE , REUTER SYLVAIN , LABORDERIE JULIEN , CLÉMENTY JACQUES , HAÏSSAGUERRE MICHEL 
Published: September 2005

Introduction: Organized atrial arrhythmias following atrial fibrillation (AF) ablation are typically due to recovered pulmonary vein (PV) conduction or reentry at incomplete ablation lines. We describe the role of nonablated anterior left atrium (LA) in arrhythmias observed after AF ablation. Methods: A total of 275 consecutive patients with paroxysmal (n = 200) or chronic (n = 75) AF had PV isolation with/without additional linear ablation at the mitral isthmus (n = 106), LA roof (n = 23), or both (n = 88). Organized arrhythmias occurring after ablation were evaluated utilizing activation and entrainment mapping. Results: Fourteen patients (11 female, 65 ± 13 years, 10 chronic AF, 10 structural heart disease) demonstrated tachycardia localized to the anterior LA, an area not targeted by prior ablation. Eight had ECG features during sinus rhythm suggestive of impaired anterior LA conduction at baseline. These arrhythmias demonstrated a distinctive ECG flutter morphology in 7 of 10 (70%) with discrete −/+ or +/−/+ aspect in inferior leads. Mapping the anterior LA revealed electrograms spanning the entire tachycardia cycle length (325 ± 125 msec). Entrainment was possible in all with a postpacing interval exceeding the tachycardia cycle length by 9 ± 10 msec. Electroanatomic mapping in 6 demonstrated small reentrant circuits rotating clockwise in 4 and counterclockwise in 2. Low‐amplitude, fractionated mid‐diastolic potentials with long duration (200 ± 80 msec) occupying 63 ± 22% of the cycle length were targeted for ablation resulting in termination and subsequent noninducibility. Conclusion: Organized arrhythmias occurring after AF ablation can be due to reentrant circuits localized to the anterior LA, predominantly in females with chronic AF, structural heart disease, and abnormal atrial conduction. They are characterized by a distinctive surface ECG and highly responsive to RF ablation at the slow conduction area.
Age‐Related Changes in Human Left and Right Atrial Conduction
Authors: KOJODJOJO PIPIN , KANAGARATNAM PRAPA , MARKIDES VIAS , DAVIES D. WYN , PETERS NICHOLAS 
Published: September 2005

Introduction: Advancing age is an independent risk factor for atrial fibrillation (AF), which is considered to be initiated by ectopic triggers and maintained by an arrhythmogenic substrate. It is not known whether substrate changes produce this age‐related increase in propensity toward AF. We addressed the hypothesis that advancing age is associated with changes in biatrial electrophysiology even in patients with no history of atrial arrhythmias. Methods and Results: Patients with left‐sided accessory pathways and requiring routine electrophysiological studies were recruited. Electroanatomic mapping was performed in the left and right atria of 23 patients (age ranging from 17 to 75 years) with structurally normal hearts and no history of AF during sinus rhythm and pacing. Unlike previous studies, a trigonometric method was used to quantify wavefront propagation velocities (WPV) precisely in the direction of propagation. Refractoriness was measured at 2 cycle lengths, at three different atrial sites. Both right (r =−0.77, P < 0.0001) and left (r =−0.79, P < 0.001) atrial WPV demonstrated strongly inverse correlation with age. Furthermore, left and right WPVs were highly correlated (r = 0.66, P < 0.01), with velocities being 6.4 ± 2.2 cm/sec higher in the right atria (P < 0.01). Refractoriness was significantly correlated with increasing age only at the septum (r = 0.53, P < 0.01). Left atrial wavelength was inversely correlated with increasing age (r =−0.56, P = 0.03). P wave duration was associated with age (r = 0.42, P = 0.04) and left atrial size (r = 0.44, P = 0.04) but not atrial WPV. Conclusion: Aging human atria demonstrate progressive decline in WPV and increase in septal refractoriness. These age‐related changes in biatrial electrophysiology are likely to be important factors in the age‐related increase in AF prevalence.
Human ex vivo bone tissue strains around natural teeth vs. immediate oral implants
Authors: Cehreli Murat Cavit , Akkocaoglu Murat , Comert Ayhan , Tekdemir Ibrahim , Akca Kıvanc 
Published: September 2005

AbstractPurpose: To compare ex vivo bone tissue strains around natural teeth with immediate implants supporting unsplinted and splinted fixed prostheses.Material and methods: Six linear strain gauges were bonded on the labial marginal bone of bilateral maxillary anterior teeth in two fresh‐frozen human cadavers. The natural teeth were loaded in centric occlusion via an occlusal splint with integrated miniature load cells. Strain measurements were performed at a sample rate of 10 kHz and under a maximum load of 100 N, simultaneously monitored from a computer connected to data acquisition system. Upon extraction of the anterior teeth, Ø 4.1 mm and Ø 4.8 mm ITI® dental implants were placed into sockets of incisors and canines, respectively. Installation torque value (ITV) of each implant was measured by a custom‐made torque wrench and resonance frequency analyses (RFA) were also undertaken. Strain measurements were performed for (1) single‐tooth implant restorations, (2) unilateral splinted anterior implants, and (3) bilateral splinted anterior implants. Finally, an autopolymerizing acrylic resin was injected into the implant sockets for simulation of osseointegration and strain measurements were performed.Results: Microstrains of all groups were statistically comparable (P>0.05), although lower strains were elicited for implants in sockets of incisors in comparison with natural teeth. Microstrains around canine implants were comparable or higher than those induced around natural canines. For all groups, microstrains around canines were higher than those around lateral (P<0.05) and central incisors (P<0.01), which had similar strain levels (P>0.05). The RFA values of implants were comparable. The ITVs of implants in sockets of lateral incisors and canines were similar and were higher than those of implants in central incisor regions.Conclusion: The labial marginal bone having no contact with immediate implants may experience strain gradients within physiologic levels due to deformation of bone under load. Although one prosthetic design does not seem to have clear advantages over another, splinting of implants may be considered as a safety measure for immediately loaded immediate implants, if possible.
Predisposing conditions for retrograde peri‐implantitis, and treatment suggestions
Authors: Quirynen Marc , Vogels Roel , Alsaadi Ghada , Naert Ignace , Jacobs Reinhilde , Steenberghe Daniel van 
Published: September 2005

AbstractBackground: Recent case reports introduced the term retrograde peri‐implantitis as a lesion (radiolucency) around the most apical part of an osseointegrated implant. It develops within the first months after insertion. This retrospective study aimed to find predisposing conditions for such peri‐apical lesions and to evaluate treatment strategies.Methods: All single implants (426 in the upper, 113 in the lower jaw, all Brånemark system® type) placed at the department of Periodontology of the University Hospital (Catholic University Leuven) were included in this retrospective evaluation to check the incidence of retrograde peri‐implantitis. Eventual predisposing factors such as patient characteristics (age, medical history), recipient site (local bone quality and quantity, cause of tooth loss), periodontal and endodontic conditions of neighboring teeth, implant characteristics (length, surface characteristics), and surgical aspects (guided bone regeneration, osseous fenestration, or dehiscency) were considered. Moreover, implants with retrograde peri‐implantitis were followed longitudinally to verify their treatment outcome by means of different parameters (Periotest® values (PTV), marginal bone level, radiological size of peri‐apical defect).Results: Seven implants in the upper (1.6%) and 3 in the lower jaw (2.7%) showed retrograde peri‐implantitis, before or at abutment connection. In comparison with successful implants, such peri‐apical lesions occurred preferably at sites with a history of an obvious endodontic pathology of the extracted tooth to be replaced. The incidence of retrograde peri‐implantitis was significantly higher (P<0.0001) for TiUnite® implants when compared with the machined implants (8/80 vs. 2/459). The machined implant surface, however, showed a higher failure rate (6.8%) than the TiUnite® implants (2.5%). Failures with machined surfaces preferably occurred at extraction sites of teeth with a history of endodontic pathology or sites adjacent to teeth with an obvious endodontic pathology. No other predisposing factors could be identified. A curettage of the peri‐apical lesions and the use of a bone substitute material prevented further progression of such lesions in the upper jaw (implants maintained their marginal bone and low PTV scores). A treatment in the lower jaw was less successful.Conclusions: Within the limitations of a retrospective study, these results seem to indicate that retrograde peri‐implantitis is provoked by remaining scar or granulomatous tissue at the recipient site: endodontic pathology of extracted tooth (scar tissue‐impacted tooth) or possible endodontic pathology from a neighboring tooth.
Influence of three currently used bone replacing materials on the in vitro proliferation of human peripheral blood mononuclear cells
Authors: Wanschitz Felix , Nell Andrea , Patruta Sanda , Wagner Arne , Ewers Rolf 
Published: September 2005

AbstractObjectives: A cell culture system for biocompatibility testing of bone grafting materials is described. We investigated the in vitro viability and proliferative response of peripheral blood mononuclear cells (PBMC) from 10 healthy donors in the presence of three materials currently used for bone grafting: Algipore, Bio‐Oss and Bone Source, for immunologic biocompatibility testing.Material and methods: PBMC isolated from venous blood from 10 healthy donors were incubated for 4 days with each bone replacing material, in the presence and absence of interleukin‐2 (IL‐2). After 4 days, H3‐thymidine was added for 18 h and the incorporated radioactivity was measured with a β‐plate counter.Results: Basal PBMC counts were 152.9±66.2 counts per minute (c.p.m.) (mean±SD), in the presence of 0.4 U IL‐2/well 206.5±83 c.p.m. were measured. With Algipore and Bio‐Oss, which are deproteinized bone replacing materials, the proliferation rate of PBMC with IL‐2 was not significantly modified: for Algipore 151±51 c.p.m./+IL‐2 188.8±62 c.p.m., for Bio‐Oss 144.5±64.9 c.p.m./+IL‐2 176.3±71.23 c.p.m. For Bone Source 164.2±80.4/+IL‐2 188.3±81 c.p.m. were measured.Conclusion: This in vitro experiment indicates, that the investigated bone replacing materials are not acting as specific antigens/haptens and are not generating increased proliferative responses of human PBMC from healthy donors. Even with IL‐2, that induces proliferation of T lymphocytes, which encountered their specific antigen, the proliferation rate of PBMC from healthy donors was not increased after incubation with this bone grafting materials.
Guided bone regeneration around non‐submerged implants in narrow alveolar ridges: a prospective long‐term clinical study
Authors: De Boever Annemarie L. , De Boever Jan A. 
Published: September 2005

AbstractObjectives: This prospective clinical study investigates long‐term survival and clinical parameters of non‐submerged implants with large buccal dehiscences treated with a deproteinized bovine bone mineral xenograft and a non‐resorbable membrane in a one‐stage approach.Material and methods: Sixteen consecutive non‐submerged implants (ITI Straumann) were installed in narrow alveolar ridges in 13 patients (age range: 25–61 years). All patients were non‐smokers. On the buccal site the bone dehiscence ranged between 3 and 9 mm. Primary stability was achieved in all but one implant. The exposed threads were covered with a xenograft (Bio‐Oss®) and a non‐resorbable expanded polytetrafluoroethylene membrane. The flap was sutured leaving the implant head non‐submerged. The membrane was removed when (1) the membrane became exposed or (2) after a maximum of 24 weeks. All implants received singular cemented crowns. The implants were followed for a period ranging from 12 to 114 months. Whole‐mouth plaque index (Pl), the % of bleeding on probing (BOP), probing depth and signs of peri‐implantitis were recorded. Every year periapical radiographs were taken using a long cone technique.Results: All but one implant integrated successfully. At the time of membrane removal, all previously exposed threads were completely covered with richly vascularized tissue except for two implants where the coverage reached 63% and 87%, respectively. The whole‐mouth plaque score and BOP remained low in all patients during the observation period. None of the implants had plaque and, except for one implant BOP never occurred. All implants were stable and in function. Swelling, redness or purulence was never observed. On the periapical radiographs no bone resorption was observed on the mesial and distal site except for one implant in one patient with a mesial and distal bone resorption of 2 and 3 mm. Probing depth was never higher than 3 mm except for one patient where the implant was placed deeply subgingival for esthetical reasons.Conclusion: This prospective long‐term study shows that with the use of non‐submerged transmucosal implants, large bony dehiscences can be treated in a one‐stage approach using a stiff non‐resorbable membrane combined with a xenograft.
Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface
Authors: Kim Hyo Jin , Chang Ik Tae , Heo Seong Joo , Koak Jai Young , Kim Seong Kyun , Jang Jun Hyeog 
Published: September 2005

AbstractObjectives: We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9‐10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE‐85 cells in the SMF were observed.Material and methods: Various magnetic fields – 1, 2, 3, 5, 7, 10 mT – were established by controlling the distance from Nd–Fe–B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 μM hFNIII9‐10 at 37°C overnight under magnetic field. The adsorbed hFNIII9‐10 was measured as optical density (OD). For attachment study, TE‐85 cells were incubated for 2 h on the hFNIII9‐10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE‐85 cell was also measured as OD value. Attachments of TE‐85 cells under various intensities of magnetic field were observed using a scanning electron microscope.Results: The amount of adsorbed hFNIII9‐10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE‐85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE‐85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE‐85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).Conclusion: Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
MITF and cell proliferation: the role of alternative splice forms
Authors: Bismuth Keren , Maric Dragan , Arnheiter Heinz 
Published: September 2005

SummaryRecent studies show that the melanocyte transcription factor MITF not only activates differentiation genes but also genes involved in the regulation of the cell cycle, suggesting that it provides a link between cell proliferation and differentiation. MITF, however, comes in a variety of splice isoforms with potentially distinct biological activities. In particular, there are two isoforms, (−) and (+) MITF, that differ in six residues located upstream of the DNA binding basic domain and show slight differences in the efficiency with which they bind to target DNA. Using in vitro BrdU incorporation assays and FACS analysis in transiently transfected cells, we show that (+) MITF has a strong inhibitory effect on DNA synthesis while (−) MITF has none or only a mild one. The strong inhibitory activity of (+) MITF is not influenced by a number of mutations that modulate MITF's transcriptional activities and is independent of the protein's carboxyl terminus but dependent on its aminoterminus. A further dissection of the molecule points to the importance of an aminoterminal serine, serine‐73, which in both isoforms is phosphorylated to comparable degrees. The results suggest that one or several aminoterminal domains cooperate with the alternatively spliced hexapeptide to render MITF anti‐proliferative in a way that does not depend on direct E box binding.
OCA4: evidence for a founder effect for the p.D157N mutation of the MATP gene in Japanese and Korean
Authors: Inagaki Katsuhiko , Suzuki Tamio , Ito Shiro , Suzuki Noriyuki , Fukai Kazuyoshi , Horiuchi Toshiyuki , Tanaka Toshihiko , Manabe Etsuko , Tomita Yasushi 
Published: September 2005

SummaryOculocutaneous albinism type 4 (OCA4) was identified as a rare form of human OCA among a group of autosomal recessive hypopigmentary disorders. Little is known about the prevailing distribution of patients of OCA4 with mutations of the MATP gene, although one Turkish, five German, one Korean, and 18 Japanese patients have been reported so far. The p.D157N mutation was previously reported to be the most frequent (0.389; 14/36) in Japanese patients and was also found in a Korean patient. On the other hand, this mutation has not been found in Turkish and German patients. We therefore investigated haplotypes of the patients who have the p.D157N mutation. The results showed that OCA4 is prevalent in East Asia including Japan and Korea likely as a result of a founder effect for the p.D157N mutation. Furthermore, it is suspected that the p.D157N mutation might have occurred on an ancestral chromosome after the divergence of Orientals and Caucasians.
Upstream stimulating factors: highly versatile stress‐responsive transcription factors
Authors: Corre Sébastien , Galibert Marie‐Dominique 
Published: September 2005

SummaryUpstream stimulating factors (USF), USF‐1 and USF‐2, are members of the eucaryotic evolutionary conserved basic‐Helix‐Loop‐Helix‐Leucine Zipper transcription factor family. They interact with high affinity to cognate E‐box regulatory elements (CANNTG), which are largely represented across the whole genome in eucaryotes. The ubiquitously expressed USF‐transcription factors participate in distinct transcriptional processes, mediating recruitment of chromatin remodelling enzymes and interacting with co‐activators and members of the transcription pre‐initiation complex. Results obtained from both cell lines and knock‐out mice indicates that USF factors are key regulators of a wide number of gene regulation networks, including the stress and immune responses, cell cycle and proliferation, lipid and glucid metabolism, and in melanocytes USF‐1 has been implicated as a key UV‐activated regulator of genes associated with pigmentation. This review will focus on general characteristics of the USF‐transcription factors and their place in some regulatory networks.