bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastroschisis ultrasound bowel characteristics demonstrate minimal impact on perinatal outcomes
Authors: Simon R. , Shay R. , Bergam B. , Katz R. , Delaney S. 
Published: December 2023

BACKGROUND: Bowel dilation and bowel wall thickness are common prenatal ultrasound measurements for fetuses with gastroschisis. Data regarding antenatal sonographic bowel findings and postnatal outcomes are conflicting. Our objective was to evaluate the impact of in utero bowel measurements on perinatal outcomes in gastroschisis pregnancies. METHODS: Retrospective cohort study of 116 pregnancies complicated by gastroschisis between 2011 and 2020. We reviewed ultrasounds documenting fetal bowel measurements. To evaluate the association of these measurements with antepartum and delivery outcomes, we ran logistic and linear models using generalized estimating equations. RESULTS: Eleven perinatal outcomes reached statistical significance, although with minimal clinical impact given small magnitude of effect. Intra-abdominal bowel dilation was associated with a 0.5 week decrease in delivery gestational age (GA) (95% CI –0.07, –0.03) and a 6.93 g increase in birth weight (95% CI 1.54, 28.73). Intra-abdominal bowel wall thickness was associated with later GA of non-stress test (NST) start of 0.22 weeks (95% CI 0.07, 0.37), increased delivery GA of 0.08 weeks (95% CI 0.02, 0.15), 0.006 decrease in umbilical artery (UA) pH (95% CI –0.009, –0.003), 0.26 increase in UA base deficit (95% CI 0.09, 0.43), and decreased odds of cesarean delivery (OR = 0.83, 95% CI 0.70, 0.99). Extra-abdominal bowel wall thickness was associated with a 0.1 increase in UA base deficit (95% CI 0.02, 0.19) and a 0.05 increase in 5-min APGAR score (95% CI 0.01, 0.09). Stomach cross-section was associated with a 0.01 week decrease in delivery GA (95% CI –0.02, –0.001) and increased odds of receiving betamethasone (OR = 1.02, 95% CI 1.01, 1.04). CONCLUSIONS: In utero bowel characteristics reached statistical significance for several outcomes, but with minimal meaningful clinical differences in outcomes.
The relationship between mean platelet volume (MPV) and intraventricular hemorrhage in very low birth weight infants
Authors: Sadeghzadeh Mansour , Khoshnevisasl Parisa , Fallah Ramezan , Marzban Asghar , Ghodrati Davood 
Published: December 2023

BACKGROUND: Intra ventricular hemorrhage (IVH) is a major cause of mortality among very low birth weight infants. This study aimed to investigate the relationship between mean platelet volume (MPV) and IVH in very low birth weight infants. METHOD: In this cohort study, all premature infants weighing less than 1500 grams who were admitted to the NICU were enrolled from August 2018 to July 2019. Brain ultrasonography was performed on the first and seventh days and one month of age and were compared with Platelet count and MPV. RESULTS: From 183 neonates, 23 cases (12.6%) had IVH. The mean MPV on the first day in the group with IVH was 10.48±0.85 and in the non-IVH group was 9.9±0.74 ( P = 0.005). The mean MPV on the seventh day in the IVH and non-IVH group were 10.4±0.92 and 10.03±0.74 ( P = 0.04) respectively. On the first and seventh day, for one unit of MPV increase, the chance of IVH increased by 2.68 and 2.1 respectively. ( p = 0.001; OR3.643; 95% CI [ 1.703 – 7.753 ]). CONCLUSION: The mean MPV index is significantly higher in the infants with IVH and therefore it can be used in early detection of IVH in neonates weighing less than 1500 grams.
Workshop Pembuatan E-Book Pembelajaran IPS Menggunakan Aplikasi Flipbook Bagi Guru Fogipsi Wilayah Kalimantan Barat
Authors: Barella Yusawinur , Wiyono Hadi , Buwono Sri , Aminuyati  , Karolina Venny , Fafizi Muhammad 
Published: December 2023

Pengabdian Kepada Masyarakat ini dilaksanakan terhadap25 orang guru IPS yang tergabung dalam FOGIPSI wilayah Kalimantan Barat, dalam bentuk pelatihan dan pendampingan Pembuatan Ebook pembelajaran IPS Menggunakan Aplikasi Flipbook. Adapun tujuan dari pelaksanaan Pengabdian ini adalah agar guru dapat mengetahui fungsi fitur dalam aplikasi flipbook, mengetahui urutan tahapan pembuatan ebook IPS dengan aplikasi flipbook, dan dapat mengetahui cara mengaitkan audio, gambar dan video dalam ebook IPS dengan aplikasi flipbook. Metode yang diterapkan dalam pelaksanaan pengabdian ini mencakup persiapan, sosialiasi terhadap partisipan, pelatihan dan demonstrasi, praktek, dan presentasi. Adapun hasil dari Pelaksanaan pengabdian ini adalah 1). Peserta yang merupakan guru IPS mampu mengenali fungsi fitur dari aplikasi Flipbook; 2) para guru mampu memahami urutan tahapan dari pembuatan e-book IPS dengan aplikasi Flipbook; dan para guru tersebut mampu untuk mengaitkan suara dan gambar pada e-book yang telah dibuat.
Peran Neuregulin-1 (NRG-1), Acidic Fibroblast Growth Factor (FGF), dan Vascular Endothelial Growth Factor (VEGF) pada Sel Perisit sebagai Inovasi Terbaru Penanganan Infark Miokard akibat Aterosklerosis
Authors: Ivan Ignatius , Widjanarko Nicolas Daniel , Sudharta Harvey , Stella Maureen Miracle , Tandarto Kevin , Santosa Mariani 
Published: December 2023

Infark miokard telah mengakibatkan 17 juta kematian di seluruh dunia pada tahun 2005. Hal ini terutama disebabkan karena terjadinya nekrosis sel miokard akibat iskemia jangka panjang. Agen trombolitik tidak memiliki pengaruh dalam meregenerasi pembuluh darah yang pecah. Oleh karena itu, banyak sel induk pembuluh darah, terutama perisit, dibawa untuk merangsang pembangunan, pematangan, rekonstruksi, dan mempertahankan permeabilitas pembuluh baru. Di sisi lain, beberapa faktor pertumbuhan seperti Neuregulin-1 (NRG-1), Acidic Fibroblas Growth Factor (FGF), dan Vascular Endothelial Growth Factor (VEGF) juga memainkan peran dalam angiogenesis yang merupakan jalur utama proses penyembuhan akibat iskemia. Tujuan dari kajian ilmiah ini adalah untuk menjelaskan peran perisit yang distimulasi oleh leptin, bersama dengan beberapa faktor pertumbuhan yang diselubungi oleh mikropartikel sebagai terapi baru infark miokard yang terutama disebabkan oleh aterosklerosis. Hasil kajian menunjukkan bahwa perisit memiliki kemampuan untuk berdiferensiasi menjadi sel otot polos, sel saraf, dan sel endotel. Proses ini dipengaruhi oleh leptin dan juga beberapa faktor pertumbuhan yang diselubungi oleh mikropartikel untuk meningkatkan stabilitas dan imunitas terhadap makrofag. Terapi sel punca dengan menggunakan perisit yang distimulasi oleh leptin dan faktor pertumbuhan yang diselubungi oleh mikropartikel dapat menjadi metode pengobatan infark miokard yang menjanjikan di masa depan.
A co-designed, community-based intensive health behavior intervention promotes participation and engagement in youth with risk factors for type 2 diabetes
Authors: Pike Julie M. , Haberlin-Pittz Kathryn M. , Alharbi Basmah S. , Perkins Susan M. , Hannon Tamara S. 
Published: December 2023

BackgroundObesity among youth (children and adolescents) is associated with increased risk for youth-onset type 2 diabetes. Lifestyle change can delay or prevent the development of type 2 diabetes, yet real-world implementation of health behavior recommendations is challenging. We previously engaged youth with risk factors for type 2 diabetes, their caregivers, and professionals in a human-centered design study to co-design a lifestyle change program. Here we report the outcomes for this 16-week co-designed lifestyle change program for youth at risk for T2D and their caregivers.Research design and methodsThis single-arm family-based cohort study included youth aged 7-18 years, with BMI ≥85th percentile (overweight or obese) and at least one additional risk factor for type 2 diabetes, and their caregivers. Clinical (BMI, HbA1c), self-reported physical activity, and quality of life outcomes were evaluated at baseline (B), post-intervention (M4), and 1 year (M12) following the intervention.ResultsSeventy-eight youth (mean age 12.4 ± 2.7y, 67% female, 37.8% white) and 65 caregivers were included in the data analysis. Youth baseline BMI z-scores (2.26 ± 0.47) and HbA1c (5.3 ± 0.3) were unchanged at follow up time points [BMI z-scores M4 (2.25 ± 0.52), M12 (2.16 ± 0.58), p-value 0.46], [HbA1c M4 (5.3 ± 0.3), M12 (5.2 ± 0.3), p-value (0.04)]. Youth reported increased physical activity at M4 (p = 0.004), but not at M12. Youth quality of life scores increased at M12 (p=0.01). Families who attended at least one session (n=41) attended an average of 9 out of 16 sessions, and 37 percent of families attended 13 or more sessions.ConclusionA co-designed, community-based lifestyle intervention promotes increased physical activity, improved quality of life, maintenance of BMI z-scores and HbA1c, and engagement in youth with risk factors for T2D.
Performance of next-generation sequencing for diagnosis of blood infections by Klebsiella pneumoniae
Authors: Wang Lei , Liu Na , Zhang Lin , Cui Likun , Zhu Mengdie , Li Zhifang , Wang Panpan , Wang Zhengbin 
Published: December 2023

ObjectiveKlebsiella pneumoniae (Kp) bloodstream infections (BSI) can be a life-threatening opportunistic infection. We aimed to evaluate the diagnostic accuracy of metagenomic next-generation sequencing (mNGS) for Kp BSI.MethodsWe retrospectively analyzed 72 patients suspected with bloodstream infection and mNGS Kp positive in peripheral blood, who were hospitalized in our hospital from January 2022 to January 2023. Clinical data and laboratory parameters were collected. All patients had blood drawn and other samples for blood mNGS, blood cultures (BC) and other cultures (OC). The accuracy of mNGS results was analyzed according to infection site, clinical indicators, therapeutic effect and routine culture results. The detection of pathogenic microorganisms by blood mNGS and routine culture was compared.ResultsAmong 72 infection patients, 29 cases (40.28%) were BC positive, 43 cases (59.72%) were other culture (OC) positive, 16 cases (22.22%) were both BC and OC positive, 56 cases were positive for both mNGS and routine culture. Among the 56 double-positive cases, mNGS and conventional cultures were completely consistent in 27 cases, partially consistent in 15 cases, and completely inconsistent in 14 cases. Using the clinical diagnosis as the reference standard, There were 51 cases consistent with the results of mNGS with Kp BSI, the clinical consistency was 70.83% (51/72). The coincidence rate of mNGS and clinical diagnosis was higher than that of BC (54.17%, 39/72), indicating a statistically significant difference between the two methods (P&lt;0.01).ConclusionsCurrent evidence indicates that mNGS exhibits excellent accuracy for the diagnosis of Kp BSI. Although it cannot replace blood culture detection technology, it can be used as a supplement to provide stronger diagnostic capabilities for BSI and optimize treatment.
Carbapenemase genes distribution in clonal lineages of Acinetobacter baumannii: a comprehensive study on plasmids and chromosomes
Authors: Beig Masoumeh , Badmasti Farzad , Solgi Hamid , Nikbin Vajihe Sadat , Sholeh Mohammad 
Published: December 2023

BackgroundThe global spread of plasmids carrying carbapenemase genes within carbapenem resistant Acinetobacter baumannii (CRAB) strains poses a worldwide public health issue. In this study, we conducted a comprehensive genetic analysis of plasmids and chromosomes harboring the major carbapenemase genes (blaNDM, blaKPC, blaVIM, blaIMP, blaGES, blaOXA-58-like, blaOXA-24/40-like, blaOXA-143-like, and blaOXA-23-like) in CRAB strains using bioinformatic tools.MethodsWe retrieved plasmids and chromosomes carrying the major carbapenemase genes from GenBank. The size, replicon type, and conjugal apparatus of the plasmids were also determined. Furthermore, allele types, co-existence of other antimicrobial resistance genes alongside carbapenemases in plasmids or chromosomes, co-occurrence of carbapenemase genes, gene repetition, and sequence types (ST) of whole genomes were characterized.ResultsThe database contained 113 plasmids and 38 chromosomes harboring carbapenemase genes. This investigation revealed that blaNDM and blaOXA-58-like were the predominant allele types in both the plasmids and chromosomes. Nine (7.96%) plasmids with blaNDM-1 were potentially conjugative. The most common replicon types of the plasmids were R3-T1, R3-T8, R3-T2, R3-T23, and RP-T1. The analysis revealed that blaNDM-1 and blaOXA-58-like genes possessed the highest variety of co-existence with other antibiotic resistance genes. The co-occurrence of dual carbapenemases was identified in 12 plasmids and 19 chromosomes. Carbapenemase gene repetitions were identified in 10 plasmids and one chromosome. Circular alignment revealed that the plasmids carrying the co-occurrence of blaNDM-1 and blaOXA-58 were more homogeneous. However, there was heterogeneity in certain regions of these plasmids. According to the minimum spanning tree (MST) results, the majority of the plasmids belonged to the genomes of ST2Pas, ST1Pas, ST422Pas, ST622Pas, and ST85Pas.ConclusionA. baumannii appears to have a strong ability for genome plasticity to incorporate carbapenemase genes on its plasmids and chromosomes to develop resistance against carbapenems. Mobilizable plasmids harboring carbapenemases significantly contribute to the dissemination of these genes. The genetic structure of the plasmids revealed a strong associations of class I integrons, ISAba-like structures, Tn4401 elements, and aac (6′)-Ib with carbapenemases. Furthermore, gene repetition may also be associated with carbapenem heteroresistance.
Development of an interpretable machine learning-based intelligent system of exercise prescription for cardio-oncology preventive care: A study protocol
Authors: Gao Tianyu , Ren Hao , He Shan , Liang Deyi , Xu Yuming , Chen Kecheng , Wang Yufan , Zhu Yuxin , Dong Heling , Xu Zhongzhi , Chen Weiming , Cheng Weibin , Jing Fengshi , Tao Xiaoyu 
Published: December 2023

BackgroundCardiovascular disease (CVD) and cancer are the first and second causes of death in over 130 countries across the world. They are also among the top three causes in almost 180 countries worldwide. Cardiovascular complications are often noticed in cancer patients, with nearly 20% exhibiting cardiovascular comorbidities. Physical exercise may be helpful for cancer survivors and people living with cancer (PLWC), as it prevents relapses, CVD, and cardiotoxicity. Therefore, it is beneficial to recommend exercise as part of cardio-oncology preventive care.ObjectiveWith the progress of deep learning algorithms and the improvement of big data processing techniques, artificial intelligence (AI) has gradually become popular in the fields of medicine and healthcare. In the context of the shortage of medical resources in China, it is of great significance to adopt AI and machine learning methods for prescription recommendations. This study aims to develop an interpretable machine learning-based intelligent system of exercise prescription for cardio-oncology preventive care, and this paper presents the study protocol.MethodsThis will be a retrospective machine learning modeling cohort study with interventional methods (i.e., exercise prescription). We will recruit PLWC participants at baseline (from 1 January 2025 to 31 December 2026) and follow up over several years (from 1 January 2027 to 31 December 2028). Specifically, participants will be eligible if they are (1) PLWC in Stage I or cancer survivors from Stage I; (2) aged between 18 and 55 years; (3) interested in physical exercise for rehabilitation; (4) willing to wear smart sensors/watches; (5) assessed by doctors as suitable for exercise interventions. At baseline, clinical exercise physiologist certificated by the joint training program (from 1 January 2023 to 31 December 2024) of American College of Sports Medicine and Chinese Association of Sports Medicine will recommend exercise prescription to each participant. During the follow-up, effective exercise prescription will be determined by assessing the CVD status of the participants.Expected outcomesThis study aims to develop not only an interpretable machine learning model to recommend exercise prescription but also an intelligent system of exercise prescription for precision cardio-oncology preventive care.EthicsThis study is approved by Human Experimental Ethics Inspection of Guangzhou Sport University.Clinical trial registrationhttp://www.chictr.org.cn, identifier ChiCTR2300077887.
Sleep disturbance exacerbates atherosclerosis in type 2 diabetes mellitus
Authors: Fan Bingge , Tang Ting , Zheng Xiao , Ding Haixia , Guo Peng , Ma Hongqing , Chen Yu , Yang Yichao , Zhang Lihui 
Published: December 2023

BackgroundShort sleep duration and poor sleep quality are important risk factors for atherosclerosis. The use of smart bracelets that measure sleep parameters, such as sleep stage, can help determine the effect of sleep quality on lower-extremity atherosclerosis in patients with type 2 diabetes.ObjectiveTo investigate the correlation between sleep disorders and lower-extremity atherosclerosis in patients with type 2 diabetes.MethodsAfter admission, all patients were treated with lower-extremity arterial ultrasound and graded as having diabetic lower-extremity vascular lesions according to the results. A smart bracelet was used to obtain the patient sleep data. The correlation between sleep patterns and diabetic lower-extremity atherosclerosis, diabetic foot, and various metabolic indices was verified.ResultsBetween August 2021 and April 2022, we screened 100 patients with type 2 diabetes, with 80 completing sleep monitoring. Univariate ordered logistic regression analysis indicated that patients with a sleep score below 76 (OR = 2.707, 95%CI: 1.127–6.488), shallow sleep duration of 5.3 h or more (OR=3.040, 95 CI: 1.005–9.202), wakefulness at night of 2.6 times or more (OR = 4.112, 95%CI: 1.513–11.174), and a deep sleep continuity score below 70 (OR = 4.141, 95%CI: 2.460–615.674) had greater risk of high-grade lower limb atherosclerosis. Multivariate ordinal logistic regression analysis revealed that the risk of high-grade lower limb atherosclerosis was higher in patients with 2.6 or more instances of nighttime wakefulness (OR = 3.975, 95%CI: 1.297–12.182) compared with those with fewer occurrences. The sleep duration curve of patients with different grades of diabetic lower-extremity atherosclerosis was U-shaped. According to the results of the one-way analysis of variance, the higher the deep sleep continuity score, the lower the Wagner scale score for diabetic foot (P &lt; 0.05).ConclusionsSleep disorders (long, shallow sleep duration, frequent wakefulness at night, and poor continuity of deep sleep) can worsen lower limb atherosclerosis in patients with type 2 diabetes. This finding can provide a new method for medical professionals to prevent and treat diabetic lower-extremity vascular lesions.
Rationale and design of the Children's Oncology Group study AAML1831 integrated cardiac substudies in pediatric acute myeloid leukemia therapy
Authors: Leger Kasey J. , Robison Nora , Narayan Hari K. , Smith Amanda M. , Tsega Tenaadam , Chung Jade , Daniels Amber , Chen Zhen , Englefield Virginia , Demissei Biniyam G. , Lefebvre Benedicte , Morrow Gemma , Dizon Ilona , Gerbing Robert B. , Pabari Reena , Getz Kelly D. , Aplenc Richard , Pollard Jessica A. , Chow Eric J. , Tang W. H. Wilson , Border William L. , Sachdeva Ritu , Alonzo Todd A. , Kolb E. Anders , Cooper Todd M. , Ky Bonnie 
Published: December 2023

BackgroundPediatric acute myeloid leukemia (AML) therapy is associated with substantial short- and long-term treatment-related cardiotoxicity mainly due to high-dose anthracycline exposure. Early left ventricular systolic dysfunction (LVSD) compromises anthracycline delivery and is associated with inferior event-free and overall survival in de novo pediatric AML. Thus, effective cardioprotective strategies and cardiotoxicity risk predictors are critical to optimize cancer therapy delivery and enable early interventions to prevent progressive LVSD. While dexrazoxane-based cardioprotection reduces short-term cardiotoxicity without compromising cancer survival, liposomal anthracycline formulations have the potential to mitigate cardiotoxicity while improving antitumor efficacy. This overview summarizes the rationale and methodology of cardiac substudies within AAML1831, a randomized Children's Oncology Group Phase 3 study of CPX-351, a liposomal formulation of daunorubicin and cytarabine, in comparison with standard daunorubicin/cytarabine with dexrazoxane in the treatment of de novo pediatric AML.Methods/designChildren (age &lt;22 years) with newly diagnosed AML were enrolled and randomized to CPX-351-containing induction 1 and 2 (Arm A) or standard daunorubicin and dexrazoxane-containing induction (Arm B). Embedded cardiac correlative studies aim to compare the efficacy of this liposomal anthracycline formulation to dexrazoxane for primary prevention of cardiotoxicity by detailed core lab analysis of standardized echocardiograms and serial cardiac biomarkers throughout AML therapy and in follow-up. In addition, AAML1831 will assess the ability of early changes in sensitive echo indices (e.g., global longitudinal strain) and cardiac biomarkers (e.g., troponin and natriuretic peptides) to predict subsequent LVSD. Finally, AAML1831 establishes expert consensus-based strategies in cardiac monitoring and anthracycline dose modification to balance the potentially competing priorities of cardiotoxicity reduction with optimal leukemia therapy.DiscussionThis study will inform diagnostic, prognostic, preventative, and treatment strategies regarding cardiotoxicity during pediatric AML therapy. Together, these measures have the potential to improve leukemia-free and overall survival and long-term cardiovascular health in children with AML.
Clinical trial registration:https://clinicaltrials.gov/, identifier NCT04293562
PPARG dysregulation as a potential molecular target in adrenal Cushing's syndrome
Authors: Vetrivel Sharmilee , Tamburello Mariangela , Oßwald Andrea , Zhang Ru , Khan Ali , Jung Sara , Baker Jessica E. , Rainey William E. , Nowak Elisabeth , Altieri Barbara , Detomas Mario , Watts Deepika , Williams Tracy Ann , Wielockx Ben , Beuschlein Felix , Reincke Martin , Sbiera Silviu , Riester Anna 
Published: December 2023

BackgroundWe performed a transcriptomic analysis of adrenal signaling pathways in various forms of endogenous Cushing’s syndrome (CS) to define areas of dysregulated and druggable targets.MethodologyNext-generation sequencing was performed on adrenal samples of patients with primary bilateral macronodular adrenal hyperplasia (PBMAH, n=10) and control adrenal samples (n=8). The validation groups included cortisol-producing adenoma (CPA, n=9) and samples from patients undergoing bilateral adrenalectomy for Cushing’s disease (BADX-CD, n=8). In vivo findings were further characterized using three adrenocortical cell-lines (NCI-H295R, CU-ACC2, MUC1).ResultsPathway mapping based on significant expression patterns identified PPARG (peroxisome proliferator-activated receptor gamma) pathway as the top hit. Quantitative PCR (QPCR) confirmed that PPARG (l2fc&lt;-1.5) and related genes – FABP4 (l2fc&lt;-5.5), PLIN1 (l2fc&lt;-4.1) and ADIPOQ (l2fc&lt;-3.3) – were significantly downregulated (p&lt;0.005) in PBMAH. Significant downregulation of PPARG was also found in BADX-CD (l2fc&lt;-1.9, p&lt;0.0001) and CPA (l2fc&lt;-1.4, p&lt;0.0001). In vitro studies demonstrated that the PPARG activator rosiglitazone resulted in decreased cell viability in MUC1 and NCI-H295R (p&lt;0.0001). There was also a significant reduction in the production of aldosterone, cortisol, and cortisone in NCI-H295R and in Dihydrotestosterone (DHT) in MUC1 (p&lt;0.05), respectively.OutcomeThis therapeutic effect was independent of the actions of ACTH, postulating a promising application of PPARG activation in endogenous hypercortisolism.