bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of an automated dish preparation system for IVF and embryo culture using a mouse mode
Authors: Zhu Yan , Feng Huai L. , Jiang Man-Xi 
Published: October 2023

AbstractManual dish preparation for IVF in human fertility clinics or animal laboratories heavily relies on embryologists' experience, which can lead to occupational illness due to long-term and monotonous operation. Therefore, introducing an automated technique to replace traditional methods is crucial for improving working efficiency and reducing work burden for embryologists. In the current study in the mouse, both manual and automated methods were used to prepare IVF or embryo culture dishes. A one-way analysis of variance was conducted to compare several factors, including preparation time, qualified rates, media osmolality of dishes, fertilization rates, and embryonic development to assess the efficiency and potential of automated preparation. The results showed that automation system significantly reduced the required time and increased the efficiencies and qualified rates of dish preparation, especially for embryo culture dishes, without significantly altering medium osmolalities. There were no significant differences between two preparations in fertilization rates and embryo development in mice. Thus, automated dish preparation can improve working efficiency and qualified rates while maintaining fertilization rates and subsequent embryonic development without compromising osmolality stability of medium. It presents a superior alternative to manual preparation, reducing the workload of embryologists and facilitating the standardization of operational procedures.
Current status of phosphine resistance in Indian field populations of Tribolium castaneum and its influence on antioxidant enzyme activities
Authors: Singh Satyapriya , Nebapure Suresh M. , Taria Sukumar , Sagar Doddachowdappa , Subramanian Sabtharishi 
Published: October 2023

AbstractResistance to phosphine is widely reported in several stored product insect pests globally. However, knowledge of its prevalence and the association of antioxidant enzymes with phosphine resistance is limited. Herein, we assessed the levels of phosphine susceptibility and estimated the antioxidant enzyme activities viz., superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) in selected Indian populations of red flour beetle Tribolium castaneum (Herbst). Dose–response probit assays revealed that the LC50 values ranged from 0.038 to 1.277 mg L−1 showing 2.11 to 70.94-fold resistance to phosphine compared to susceptible check. Activities of antioxidant enzymes varied significantly between the T. castaneum populations following phosphine exposure. The magnitude of SOD activity ranged from 8.77 to18.82 U mg−1 protein, while, the activities of POX and CAT varied between 52.42 and 408.32 and 61.11 to 247.49 µM H2O2 reduced min−1 mg−1 of protein, respectively. The correlation analysis revealed a significant positive association of SOD (r = 0.89) and POX (r = 0.98) with increased resistance ratio, while the CAT (r = − 0.98) is negatively linked with resistance to phosphine. A principal component analysis identified phosphine resistance was closely associated with POX and SOD activities but was unrelated to the CAT activity. Our results throw light on the varied association of antioxidant enzyme activities in response to phosphine fumigation in field populations of T. castaneum. Further studies on the biochemical and molecular basis of phosphine stress in insects may help to devise suitable strategies to safeguard storage commodities and ensure a sustainable environment.
Multicomponent synthesis of pyrimido[4,5-b] quinolines over a carbocationic catalytic system
Authors: Moosavi-Zare Ahmad Reza , Najafi Raha 
Published: October 2023

AbstractTrityl chloride (TrCl) was efficiently used as a neutral catalyst for the multicomponent cyclization reaction of an aldehyde with dimedone and 6-amino-1,3-dimethyluracil for the preparation of pyrimido[4,5-b] quinolines in chloroform under reflux condition.
The Role of High-Value Agriculture in Capability Expansion: Qualitative Insights into Smallholder Cash Crop Production in Nepal, Laos and Rwanda
Authors: Matthys Marie-Luise , Illien Patrick , Acharya Sushant , Amacker Michèle , Bieri Sabin , Musafili Ildephonse , Sanesathid Outhoumphone 
Published: October 2023

AbstractHigh-value agriculture contributes to rural incomes, but does it also contribute to expanding “human capabilities” (Sen, Development as freedom, Knopf, New York, 1999) in a durable way? Through long-term qualitative fieldwork in three landlocked LDCs—Nepal, Rwanda and Laos—resulting in over 150 interviews, we found expansions of the three analysed capabilities: paid work, mobility and social relations. Yet, those improvements were characterised by precariousness: they were mostly not resilient in the face of the economic and environmental risks that high-value agriculture entails. The only example of a durable capability expansion was found in Nepal, where women claimed social spaces through collective organisation. All three study sites showed remarkable consistency in that the considerable risk involved in cash crop production was mainly borne by farmers and rural labourers. Research on mechanisms to guard against these risks at household or individual level is warranted.
Possible relationships between the physical conditions of cattle and the occurrence of structural modifications of their coat hair
Authors: Kawahara Yutaka , Kawahara Naoya , Yamamoto Masaki , Wakizaka Hiroyuki , Okada Michiko , Ikeda Yoshimitsu 
Published: October 2023

Context Concerning the epithelial tissue in the skin, including hair, vitamin A is required for the integrity of epithelial cells, strengthened by compounding keratin fibres. Further, the complex hormonal changes seen in pregnancy may contribute to the hair growth. Thus, the structure of the cattle coat hair may change depending on the conditions of vitamin A deficiency or pregnancy. Aims The effects of several diseases, i.e. vitamin A deficiency, pneumonia, chronic pneumonia, and chronic renal failure, or pregnancy, on the structure of the coat hair were investigated. Methods Physical properties of the coat hair, collected from Japanese black cattle (Bos Taurus) and Holstein cows (Bos taurus Taurus), were investigated to study the effects of vitamin A deficiency, other diseases, or pregnancy on the formation of hair. Key results When vitamin A concentration was lowered to about half of the minimum concentration for vitamin A adequacy of 20 μg/dL in plasma (i.e. deficiency level was high), we could perceive the interference in the self-assembly of the keratin fibrils. However, we also confirmed structural modifications of the keratin fibres obtained from the cattle suffering chronic renal failure. The coat hair collected from the pregnant cows tended to show superior tensile strengths to those of the hair from the non-pregnant ones when compared at the same diameter level, although pregnancy might have increased cortisol affecting the hair follicle function. Further, from the X-ray diffraction measurements, the non-pregnant cow coat hairs, having exhibited the inferior tensile properties, were found to be structurally modified. Conclusions The structure of the coat hair could be modified when the vitamin A concentration was severely reduced (vitamin A deficiency). The physiological conditions during pregnancy may be advantageous to serve the regular formation of the hair. Implications Hair has a function serving as a proxy of health status for a certain period of animal life.
The evolution of multiple sclerosis disease-modifying therapies: An update for pharmacists
Authors: Edinger Alexandra , Habibi Mitra 
Published: October 2023

Abstract

Purpose
Multiple sclerosis (MS) and the evolution of disease-modifying therapies (DMTs) and their indications, mechanisms of action, efficacy, pregnancy class, and cost are discussed.

Summary
MS is an immune-mediated, demyelinating, and progressive neurological disorder that can cause both motor and cognitive deficits. Onset of MS typically occurs between the ages of 20 and 40 years, and the disease can result in significant disability over time. Since the introduction of the first DMT for the treatment of MS in 1993, significant progress has been made in the development of new classes of DMTs with different mechanisms of action, higher efficacy, and simpler administration schedules, offering patients better alternatives. However, drawbacks with the use of DMTs include their increasing cost and formulary restrictions.

Conclusion
The treatment landscape of MS has significantly changed over the past 2 decades, and the introduction of newer classes of DMTs provides an opportunity for pharmacists to play an important role in the management of this patient population.
Gains and losses of the epiphytic lifestyle in epidendroid orchids: review and new analyses of succulence traits
Authors: Collobert Géromine , Perez-Lamarque Benoît , Dubuisson Jean-Yves , Martos Florent 
Published: October 2023

Abstract

Background and Aims
Epiphytism has evolved repeatedly in plants and has resulted in a considerable number of species with original characteristics. Because water supply is generally erratic compared to that in soils, succulent forms in particular are widespread in epiphytic species. However, succulent organs also exist in terrestrial plants, and the question of the concomitant evolution of epiphytism and succulence has received little attention, not even in the epidendroid orchids, which account for 67.6 % of vascular epiphytes.

Methods
We built a new time-calibrated phylogenetic tree of Epidendroideae with 203 genera treated in genus Orchidacearum, from which we reconstructed the evolution of epiphytism as well as traits related to water scarcity (stem and leaf succulence and the number of velamen layers), while testing for the correlated evolution between the two. Furthermore, we estimated the ancestral geographical ranges to evaluate the palaeoclimatic context in which epiphytism evolved.

Key Results
Epiphytism evolved at least three times: 39.0 million years ago (Mya) in the common ancestor of the Malaxideae and Cymbidieae that probably ranged from the Neotropics to Southeast Asia and Australia, 11.5 Mya in the Arethuseae in Southeast Asia and Australia, and 7.1 Mya in the neotropical Sobralieae, and it was notably lost in the Malaxidiinae, Collabieae, Calypsoeae, Bletiinae and Eulophiinae. Stem succulence is inferred to have evolved once, in a terrestrial ancestor at least 4.1 Mya before the emergence of epiphytic lineages. If lost, stem succulence was almost systematically replaced by leaf succulence in epiphytic lineages.

Conclusions
Epiphytism may have evolved in seasonally dry forests during the Eocene climatic cooling, among stem-succulent terrestrial orchids. Our results suggest that the emergence of stem succulence in early epidendroids was a key innovation in the evolution of epiphytism, facilitating the colonization of epiphytic environments that later led to the greatest diversification of epiphytic orchids.
Staminal hairs increase pollinator attraction and pollination accuracy in Tradescantia fluminensis (Commelinaceae)
Authors: Li Deng-Fei , Chen Yi-Dan , Liu Zhen , Liang Ai-Ting , Tang Ju , Yan Xian-Chun 
Published: October 2023

Abstract
Staminal hairs are the particular appendages of stamens, which may affect pollinator foraging behaviour and pollen transfer. However, experimental evidence of the functions of staminal hairs in pollination remains scarce. Here, we conducted staminal hair manipulation experiments in Tradescantia fluminensis (Commelinaceae) to investigate their effects on visitation and pollen transfer by bees. Our observations revealed that both visitation rates and visit duration of honeybees (Apis cerana) to control flowers were significantly higher than that of hairless flowers. Moreover, removing the staminal hairs significantly decreased pollen deposition by honeybees (A. cerana), but did not affect pollen removal. The staminal hair was similar in length to the stamen and the pistil of T. fluminensis. The staminal hairs provide more footholds for honeybees, and they lay prone on the staminal hairs to collect pollen, which increased the accuracy of pollination through the consistent pollen placement and pick-up on the ventral surface of honeybees. These results showed that the staminal hairs in T. fluminensis may represent an adaptation to attract pollinators and enhance pollination accuracy.
Assessment of phenotypic heterogeneity in Salmonella Typhimurium preadapted to ciprofloxacin and tetracycline
Authors: Dawan Jirapat , Kim Songrae , Ahn Juhee 
Published: October 2023

Abstract
Bacterial population exposed to stressful antibiotic conditions consists of various subpopulations such as tolerant, persister, and resistant cells. The aim of this study was to evaluate the phenotypic heterogeneity of Salmonella Typhimurium preadapted to sublethal concentrations of antibiotics. Salmonella Typhimurium cells were treated with 1/2 × MIC of antibiotics for the first 48 h and successively 1 × MIC for the second 24 h at 37°C, including untreated control (CON), no antibiotic and 1 × MIC ciprofloxacin (NON-CIP), 1/2 × MIC ciprofloxacin and 1 × MIC ciprofloxacin (CIP-CIP), 1/2 × MIC tetracycline and 1 × MIC ciprofloxacin (TET-CIP), no antibiotic and 1 × MIC tetracycline (NON-TET), 1/2 × MIC ciprofloxacin and 1 × MIC tetracycline (CIP-TET), and 1/2 × MIC tetracycline and 1 × MIC tetracycline (TET-TET). All treatments were evaluated by antibiotic susceptibility, ATP level, relative fitness, cross-resistance, and persistence. S. Typhimurium cells were more susceptible to non-adapted NON-CIP and NON-TET (&gt;3-log reduction) than pre-adapted CIP-CIP, TET-CIP, CIP-TET, and TET-TET. CON exhibited the highest ATP level, corresponding to the viable cell number. The relative fitness levels were more than 0.95 for all treatments, except for NON-CIP (0.78). The resistance to ciprofloxacin and tetracycline was increased at all treatments with the exception of NON-TET. The persister cells were noticeably induced at CIP-TET treatment, showing more than 5 log CFU mL−1. The results suggest that the antibiotic preadaptation led to heterogeneous populations including persisters that can develop to resistance. This study provides new insight in the bacterial persistence associated with their potential risk and paves the way to design antibiotic therapy targeting dormant bacteria.
Associations of Blood Absolute Neutrophil Count and Cytokines With Cognitive Function in Dementia-Free Participants: A Population-Based Cohort Study
Authors: Fa Wenxin , Liang Xiaoyan , Liu Keke , Wang Nan , Liu Cuicui , Tian Na , Zhu Min , Ma Yixun , Song Lin , Tang Shi , Cong Lin , Wang Yongxiang , Hou Tingting , Du Yifeng , Qiu Chengxuan 
Published: October 2023

Abstract

Background
The relationships of neutrophils and cytokines with cognitive dysfunction are poorly defined. We aimed to investigate the association of peripheral blood absolute neutrophil count (ANC) with cognitive function in older adults and to further explore the mediating role of serum cytokines in this association.

Methods
This population-based cohort study included 1 666 dementia-free participants (age ≥60 years) derived from baseline examinations (March–September 2018) of the Multimodal Intervention to Delay Dementia and Disability in Rural China (MIND-China); of these, 1 087 participants completed follow-up examinations in October–December 2019. We used a neuropsychological test battery to assess episodic memory, verbal fluency, attention, and executive function at the baseline and follow-up examinations. We used Mindray BC-6800 automated hematology analyzer to measure ANC and Meso Scale Discovery to measure serum interleukin-6 (IL-6) and eotaxin-3.

Results
The linear regression analysis of cross-sectional data at baseline (n = 1 666) suggested that increased ANC was significantly associated with a lower episodic memory z score (β coefficient: −0.149, 95% CI: −0.274 to −0.023) and lower long-delayed free recall z score (−0.216, −0.361 to −0.070). Serum IL-6 and eotaxin-3 could mediate 16.16% to 20.21% and 7.55% to 9.35%, respectively, of these associations. The analysis of longitudinal data (n = 1 087) showed a J-shaped relationship of ANC with decline in episodic memory z score (p for nonlinear = .049), and a U-shaped relationship between ANC and decline in long-delayed free recall z score (p for nonlinear = .043).

Conclusions
Increased neutrophils are associated with poor cognitive performance and accelerated decline in episodic memory, and the cross-sectional association is partly mediated by serum cytokines.
International parliamentary institutions and political donations: the case of the European Parliament
Authors: Katsaitis Alexander 
Published: October 2023

Abstract
International Parliamentary Institutions (IPIs) increasingly welcome non-state actors (NSAs) within their policy-making procedures. Political donations are an important tool in NSAs' lobbying toolbox that has been well researched at the national level. Nevertheless, we know little about the links between NSAs, IPIs and political finance. Employing the European Parliament as an exploratory case-study, the article meticulously assesses micro-level financial donor data. Contrasting neo-functional and intergovernmental approaches, the results demonstrate a cleavage formed between individuals supporting anti-integration political parties, and international business donating to pro-integration parties. Theoretically, the article validates postfunctionalist approaches as a model for parts of the NSA–IPI relationship. Conceptually, the analysis shows that IPIs can encourage transnational cleavage formation and serve a representative function, which addresses the concerns about democratic deficit that come with the transfer of policy-making authority to international organizations. Simultaneously, financial donations can strengthen movements that oppose an IPI, and have an asymmetric impact on the IPI's political legitimacy. This calls for further research on NSAs' political financing of IPIs, the impact of financial donations on IPIs and the need to update relevant regulatory frameworks.
Sweden, NATO and the role of diasporas in foreign policy
Authors: Aggestam Lisbeth , Schierenbeck Isabell , Wackenhut Arne 
Published: October 2023

Abstract
When and how do diasporas influence the foreign policy of liberal democratic states? Few studies have sought to conceptualize how diasporas affect strategic bilateral relations between states. We argue that these non-state actors are an increasingly important factor in western liberal democratic societies, which challenge traditional theories of foreign policy. To explore when and how the transnational societal ties and interests of diaspora groups affect foreign and security relations between states, we develop a triadic analytical model of state–diaspora interactions and specify key contextual factors. To illustrate the dynamics at play, we analyse the influence of the Kurdish diaspora in Sweden on Turkish–Swedish bilateral relations in the wake of Sweden's decision to apply for full NATO membership. Our case-study builds on semi-structured interviews as well as news media sources, speeches and official documents. The study shows that the Kurdish diaspora demonstrates an independent role and an ability to shape the policy process due to the permeability of the political system. As a result, the policy-process is becoming more domesticated, thereby constraining the role of the executive. The article contributes important insights to policy-makers and diplomats on what impact transnational state–society relations can have on foreign policymaking.
Trust and calculation in international negotiations: how trust was lost after Brexit
Authors: Wille Tobias , Martill Benjamin 
Published: October 2023

Abstract
How does a loss of trust affect international negotiations? We know that trust enables cooperation and comes with the risk of betrayal. Yet the effects of lost trust are not well understood, with most research in International Relations (IR) focusing on how trust is initially built in adversarial relations. In this article, we conceptualize trust as the eclipsing of possible futures in which others do not behave cooperatively. When trust is lost, actors include further possibilities of non-cooperative behavior in their calculations and adjust their negotiating demands accordingly. We use this framework to explain the European Commission's response to Boris Johnson's government breaking its trust during the Brexit negotiations by deviating from the Political Declaration, introducing the Internal Market Bill, and not conducting border checks in the Irish Sea. Drawing on elite interviews, we trace how the Commission altered its expectations and consequently sought negotiated outcomes with tighter contracting, reduced discretion and retaliatory mechanisms. Analysing the interplay of trust and calculation on a granular level, we show that breaches of trust do not preclude future cooperation per se, but shape its form and extent in important ways.
Identification of the immunogenic membrane proteins, catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori
Authors: Li Zhirong , Zhang Ying , Mi Chaoyi , Deng Xiaoqing , Wang Xian , Hu Dailun , Yin Kaige , Yin Changfu , Zhao Lianmei , Shan Baoen 
Published: October 2023

Abstract

Aims
This study aims to investigate the specific membrane antigens that are targeted by antibodies raised against Helicobacter pylori.

Methods and results
Bovine milk antibodies were prepared using whole H. pylori, purified membrane proteins, or both. Enzyme-linked immunosorbent assay and sodium dodecyl sulfate–polyacrylamide gel electrophoresis experiments revealed that these immunogens triggered anti-H. pylori antibody production in milk. The highest antibody titer was induced by the mixture of whole bacteria and purified membrane proteins. The antibodies induced by mixed immunogens significantly inhibited H. pylori growth in vitro and were used to identify catalase, plasminogen-binding protein A (PgbA), and PgbB via western blotting, immunoprecipitation, and two-dimensional western blotting followed by liquid chromatography with tandem mass spectrophotometry. The immunogenicity of PgbA and PgbB was verified in mice vaccinated with their B-cell epitope vaccines. Following prophylactic vaccination of C57BL/6 mice, each of the three antigens alone and their combination reduced the weight loss in mice, increased antibody titers, and relieved the inflammatory status of the gastric mucosa following H. pylori infection.

Conclusions
Catalase, PgbA, and PgbB could serve as valuable membrane antigens for the development of anti-H. pylori immunotherapies.