bluuebunny/crossref_metadata_2025_split huggingface dataset:
P1199 An Inflammatory Bowel Diseases Integrated Resources Portal (IBDIRP)
Authors: Nie K 
Published: January 2024

Abstract

Background
Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease (CD), is a chronic and debilitating gastrointestinal disorder that affects millions of people worldwide. Research on IBD has generated massive amounts of data, including literature, metagenomics, metabolism, bioresources, and databases. We aim to create an IBD Integrated Resources Portal (IBDIRP) that provides the most comprehensive resources for IBD.

Methods
An integrated platform was developed that provides information on different aspects of IBD research resources, such as single nucleotide polymorphism, genes, transcriptomics, microbiota, metabolism, single cells, and other resources. Valuable and comprehensive IBD-related data were collected from PubMed, Google, GMrepo, gutMega, gutMDisorder, Single-cell Portal, and other sources. Then, the data were systematically sorted, and these resources were manually curated.

Results
We systematically sorted and cataloged more than 312 unique risk single nucleotide polymorphism associated with IBD in the single nucleotide polymorphism section. We presented over 890 IBD-related genes based on the database collection in the gene section. We also obtained 153 manually curated IBD transcriptomics data, including 12,388 samples, on the GEO database. The sorted IBD-related microbiota data from three primary microbiome databases (GMrepo, gutMega, and gutMDisorder) were available for download. We selected 23,149 IBD-related taxonomic records from these databases. Additionally, we collected 24 IBD metabolism studies with 2,896 participants in the metabolism section. We introduced two interactive single-cell data plug-in units that provided data visualization based on cells and genes. Finally, we listed 18 significant IBD web resources, such as the official ECCO and IOIBD websites, IBD scoring tools, IBD genetic and multi-omics resources, IBD biobanks, and other useful research resources.

Conclusion
The IBDIRP website is the first integrated resource for global IBD researchers. This portal will help researchers by providing comprehensive knowledge and enabling them to reinforce the multi-dimensional impression of IBD. The IBDIRP website is accessible via www.ibdirp.com.
P1201 Association between Inflammatory Bowel Disease and Henoch-Schönlein purpura: A Bidirectional Two-Sample Mendelian Randomization Study
Authors: Yu Q , Luo J , Zhang Y , Chen J , Wang X , Lu X , lv M , Li S , Huang X , Hu W , Zhang M , Liu L , Hu Y , Xu J , Xu D , Zhang H , Chen M , Roozbahani A , Ghoushi E , Chen Y 
Published: January 2024

Abstract

Background
Henoch-Schönlein purpura (HSP) is a systemic small-vessel vasculitis, and its association with inflammatory bowel disease (IBD) has been the focus of considerable research. Despite numerous studies, it remains uncertain whether and in which direction causal relationships exist between HSP and IBD. To reveal the causal association between HSP and IBD, we conducted a bidirectional two-sample Mendelian randomization analysis using publicly available genome-wide association study (GWAS) summary statistics.

Methods
We obtained summarized data for IBD, Crohn's disease (CD), ulcerative colitis (UC), and HSP from various GWAS. To estimate causality, we used inverse-variance weighted approaches. Additionally, we conducted several sensitivity analyses. In our causality estimates, we present odds ratios (ORs) and 95% confidence intervals (CIs).

Results
We found that CD (OR: 1.12, 95% CI: 1.00 to 1. 25, P &lt; 0.05), but not UC (OR: 1.04, 95% CI: 0. 909 to 1.19, P &gt; 0.05), had significant positive causal effects on HSP risk. However, the overall result of the MR study demonstrated that there was no causal link between genetic predisposition to IBD and an increased risk of HSP (OR: 1.10, 95% CI: 0.989 to 1.23, P &gt; 0.05). Regarding the reverse directions, no significant causal associations were discovered.

Conclusion
It appears that CD and HSP are causally related, which could influence clinical decisions regarding the management of CD in patients diagnosed with HSP. However, neither overall IBD nor UC has a causal effect on HSP.
P1218 Non-invasive point-of-care stool sample for detection of Cytomegalovirus infection in patients with Active or Refractory Ulcerative Colitis using multiplex polymerase chain reaction (mPCR): a pilot feasibility study
Authors: Peneva P , Lyutakov I , Valkov H , Vladimirov B , Penchev P 
Published: January 2024

Abstract

Background
Cytomegalovirus (CMV) infection is associated with more flare-ups and deteriorates the prognosis of active inflammatory bowel disease (IBD). Patients with refractory IBD should be tested for CMV colitis, especially if they are not responding to immunosuppressive therapy. The prevalence of CMV co-infection ranges from 10% to 30% in steroid-refractory acute colitis. Concurrent CMV-colitis is associated with an increased risk of worse therapeutic outcomes, resistance to corticosteroids, and need for salvage therapy. One of the two gold standards of diagnosis of CMV colitis in IBD patients with active disease requires the analysis of tissue biopsies using PCR. The aim of our pilot study was to evaluate the diagnostic accuracy of non-invasive point-of-care faecal samples using mPCR for detecting CMV in patients with ulcerative colitis compared to standard practice biopsies PCR.

Methods
We performed a prospective, observational, feasibility study including 62 consecutive patients with active ulcerative colitis (UC) requiring intravenous steroids or steroid-refractory UC in the Department of Gastroenterology of University Hospital „Tsaritsa Yoanna – ISUL", from April 2022 to October 2023. In all UC patients, colonic tissue biopsies were performed and analyzed by qualitative CMV PCR. Faecal sample mPCR CMV was performed on the positive patient cohort to compare the feasibility of (ME) Panel IVD using FilmArray mPCR (Biofire, bioMerieux, France). All the tested patients had undergone immunosuppressive therapy and initial stool specimen cultures were obtained to exclude infection with C. difficile and other pathogen flora.

Results
Out of 62 active UC patients with colonic tissue samples qualitative CMV PCR, n=5 (8%) were positive. Of these n=5 UC patients, n=4 (80%) were positive from the Fecal sample mPCR CMV, resulting in a compatibility of 80% for diagnosing CMV in these patients by the two DNA extraction methods. Calculated Sensitivity and diagnostic accuracy was 80% (95% CI) for the mPCR CMV. The patient cohort predominantly comprised males (75%) with a median age of 45 years (range: 30–72 years). Three patients (75%) out of this cohort group were treated with peroral antiviral agent Valganciclovir, one (25%) of them went into clinical and endoscopic remission after the treatment and two patients (50%) received colectomy. The median time performing the Faecal sample mPCR CMV was approx. 1 hour.

Conclusion
This novel faecal mPCR point-of-care test is a promising, fast and easy non-invasive tool for detection CMV in active or refractory UC patients, comparable with the colonic tissue CMV-DNA PCR.
P1231 7-ketositosterol in high-temperature heating oils aggravates colitis by gut microbiota dysbiosis induced-PDLIM3 activation
Authors: Yan J , Cao H 
Published: January 2024

Abstract

Background
Diet and gut microbiota alteration are considered as the most important environmental factors triggering inflammatory bowel disease. Phytosterols are rich in edible oils, and can be oxidized to oxyphytosterols during high-temperature cooking. Previous studies have indicated that oxyphytosterols exposure exerted potential adverse effects in cell and animal models. It remains unknown whether oxyphytosterols could promote colonic inflammation. 7-ketositosterol has the highest proportion in oxyphytosterols.

Methods
The fried and baked foods intake was collected and compared in IBD patients and healthy individuals. 7-ketositosterol was synthesized from β-sitosterol and impacts of 7-ketositosterol on intestinal inflammation was evaluated in colitis mice models. 16S rRNA and transcriptome sequencing were carried out. Furthermore, fecal microbiota transplantation was carried out to evaluate the effect of gut microbiota. Protein interaction of Staphylococcus lentus and PDLIM3 was explored by co-immunoprecipitation.

Results
The fried and baked foods intake in IBD patients was much more than that of healthy individuals, with higher exposure to oxyphytosterols. 7-ketositosterol (KS) aggravated mice colitis. KS increased the abundance of Actinobacteria (p=0.023). Meanwhile, the abundance of potential pathogens such as Staphylococcus (p=0.004) and Corynebacterium_1 (p=0.003) were increased in KS group, while the abundance of short chain fatty acids producing bacteria were decreased. Transcriptome data showed KS increased the expression of PDLIM3 (p=0.004). PDLIM3 expression was demonstrated to increase in intestinal epithelial cells, and the expression tendency of p-p38 and p-p65 was consistent with PDLIM3. In addition, KS did not exacerbate DSS induced colitis after antibiotic cocktails treatment, and PDLIM3 expression decreased in parallel. The gut microbiota from KS also aggravated DSS induced colitis in antibiotic treated mice. Proteins from Staphylococcus lentus could interact with PDZ domain and promote PDLIM3 expression.

Conclusion
7-ketositosterol in high temperature heating oils such as fried foods could aggravate colitis by modulation of gut microbiota. Gut microbiota interacted with PDZ domain, promoted PDLIM3 expression and then activated p38MAPK/NF-κB signaling pathway.
P1234 The gut microbiome as a reporter of health, and its associations with different exposures
Authors: Braun T , Hadar R , Efroni G , Haaronian O , Leshem R , Amit S , Abbas-Egbariya H , Truma K , Segev S , Amir A , Haberman Ziv Y 
Published: January 2024

Abstract

Background
The prevalence of metabolic and immune-mediated diseases has increased in the last century, with many of these diseases showing alterations in the gut microbial composition. We previously reported (PMID:35197084) that a large group of bacteria shows constant differential abundance (increase and decrease) across multiple diseases and can therefore provide a general indication of the host's health. We aimed to utilize a “health index” based on these bacteria to compare how different exposures are associated with this index as potential contributors to general health.

Methods
The gut microbial composition was derived using fecal 16S sequencing (V4), and the per-participant microbial health index was calculated. Exposures, diseases, and laboratory results were recorded at sample collection.

Results
372 participants (62% males, median age 57y, Table 1) were included (the study is ongoing). Median BMI was 25 (IQR 23-28), with 9% smokers. Participants are relatively fit: only 22% reported performing physical activity less than once weekly, and the median average steps (for the 55% that reported their daily average) is 6000 steps/day (IQR 4500-8000). Subjects performing more frequent physical activity (&gt;3 times/week) had a higher gut microbial health index (p=0.009) in comparison to less than once weekly. Subjects having no documented disease (22%) had higher indexes, and the number of diseases negatively correlated with the health index (rho=-0.19, p=0.004). A higher microbial health index (Figure 1) was found in subjects drinking &gt;=4 coffee cups/day vs. less or no coffee (p=0.002). Interestingly, a higher percentage of eosinophils was positively correlated with a higher index (p=0.002). Lower health index was recorded in subjects who reported dietary supplements intake (p=0.01), those with above-normal levels of uric acid (p=0.02), with primary hypertension or fatty liver (p=0.02), and those taking beta-blockers (p=0.009) or glucose-lowering medications (p=0.003). The microbial health index positively correlated with Faith alpha diversity (rho=0.47). Still, in some cases, each factor was linked with different features suggesting that these microbial-based factors (diversity and health index) captured a complementary epiphenomenon of the microbiome. For example, only higher alpha diversity (but not health index) was linked with the daily bowel movement frequency (p=0.0008).

Conclusion
Using the gut microbial health index, we developed a methodology to assess correlations between different lifestyles, diets, and health. This approach may highlight personal modifiable factors that can be translated into risk prevention strategies for improving general health reported by microbial composition.
P1242 Pediatric IBD microbiome and microbial profiles formed within the family
Authors: Dovrolis N , Moschoviti A , Karamanolis G , Fessatou S , Kolios G , Gazouli M 
Published: January 2024

Abstract

Background
The rise of microbiome-focused research, reveals that the intrafamilial microbiome can be linked to specific genetic traits and be studied for its behavior during dysbiosis. It has been reported that microbiota composition is shared between cohabitating individualsand displays patterns of similarity that differ between families. Based on these observations, this study focuses on treatment-naïve pediatric IBD patients and their immediate families to identify the role of the microbiome in disease onset.

Methods
Families with pediatric IBD were recruited,comprising seven drug-naïve Crohn’s disease (CD) patients and two drug-naïve ulcerative colitis (UC) patients, as well as healthy siblings/parents. Fecal samples were collected for 16S ribosomal RNA gene sequencing and bioinformatics analysis.

Results
Our results elucidate the fact that even though family members share lifestyle and dietary habits, the patients exhibit unique microbiome patterns which could only be attributed to the disease. In addition, we studiedthe impact of the disease on specific microbial taxa and how these could serve as potential biomarkers for early detection.

Conclusion
We found a potential role of maternal factors in the establishment and modulation of the early life microbiome, consistent with the current literature, which may have implications for understanding the etiology and progression of IBD.
N05 Are we meeting the sexual wellbeing concerns of patients with IBD and a stoma? A survey on current practice in addressing sexual wellbeing
Authors: Fourie S , Czuber-Dochan W , Norton C 
Published: January 2024

Abstract

Background
People with inflammatory bowel disease have a high incidence of stoma formation, either temporary or permanent. Although a stoma can be a life saver, for some it can negatively impact body image and psycho-emotional wellbeing. Sexual function can also be affected by a poor body image. Therefore, intimacy and sexuality should be addressed in clinical settings during stoma care. We aimed to explore the experiences of sexual wellbeing concerns of people living with IBD and a stoma, and how these were addressed by healthcare professionals.

Methods
This was Stage 1 of an international mixed method study. Data were collected via an anonymous online survey advertised on social media platforms and charity webpages.

Results
These are the quantitative results. 134 participants with IBD responded: 11 had colostomy and 123 had ileostomy. Time from stoma formation to survey response was between 2 weeks to 32 years. Peristomal hernia was reported by 27.6% (n=37) participants. Only 30.5 % (n=41) reported that sexual wellbeing was discussed around the time of their stoma formation, mainly with their surgeon or stoma nurse. Some 33.5% (n=45) reported having had no intimacy and sexuality concerns at all at the time of stoma formation. The patient perspective on barriers for asking healthcare professionals (HCPs) questions about sexual wellbeing despite having concerns, were reported as being ashamed to ask, afraid of being judged by their HCPS, and not knowing what to ask as being overwhelmed with technical information related to stoma care. Around 66% (n=89) reported having concerns related to their intimacy and sexuality following stoma formation, with the top three reported concerns being appearance/body image, fear of rejection from partner, and bag leakages. All participants reported wanting sexual wellbeing discussed routinely at any point of care, and the majority wanted partners involved in these discussions.

Conclusion
Patients expect to be informed about intimacy and sexuality aspects as an integral part of stoma care. Current practice does not meet the patient’s care needs, with potential for having a negative impact on their overall quality of life, since a significant number of ostomates report sexual wellbeing concerns after the stoma formation.Sexual wellbeing conversations should be an integral part of perioperative and stoma care to prevent persistent concerns within this patient group. Not providing adequate information during stoma care about sexual wellbeing can negatively impact their quality of life. The results will form the basis of an interview guide for the second (interview) stage of the study, to understand better the reasons behind these findings.
N22 Compliance with Anti-TNF Alpha Treatment and Quality of Life of Inflammatory Bowel Patients: A Descriptive and Correlational Study
Authors: Sözeri Öztürk E , Uçan Ş , Karakan T 
Published: January 2024

Abstract

Background
Today, anti-TNF agents are often preferred in the treatment of inflammatory bowel diseases. During this long-term treatment, patients' compliance with the treatment is very important. This study aims to determine the relationship between compliance with anti-TNF alpha treatment and quality of life in inflammatory bowel patients.

Methods
This study is currently being carried out at Gazi Hospital Gastroenterology Department, Inflammatory Bowel Diseases Outpatient Clinic. The study will be carried out between November and February 2024. Data are collected using the "Patient Information Form", "Inflammatory Bowel Diseases Quality of Life Scale", "Anti-TNF Alpha Treatment Compliance Scale". Whether the variables were suitable for normal distribution was determined by the Kolmogorov-Smirnov test. Since parametric test assumptions were not met, Mann-Whitney U test, Kruskal Wallis test and Sperman correlation analysis were used in the analysis of scale scores. Statistical significance value was accepted as p&lt;0.05.

Results
In this section, the pilot results of the study are presented and its implementation is ongoing (Number of patients reached: 117). The mean age of the patients participating in the study was determined to be 38.99±13.73 (minimum=18, maximum=68). It was determined that 53.7% of the patients were male, 57.3% were married and 42.7 of them had no children. The diagnosis of 65% of patients is Crohn's disease. The rate of patients being diagnosed with inflammatory bowel disease for 3-5 years was found to be 29.9%. The total score of the Inflammatory Bowel Diseases Quality of Life Scale was found to be 160.04±32.24 (minimum=88.00, maximum=224.00). The total score of the Anti-TNF Alpha Treatment Compliance Scale was found to be 2.36±0.72 (minimum=1.00, maximum=4.58). It was determined that there was a strong negative relationship between the total scores of the scales (p= &lt;0.001).

Conclusion
It can be said that the treatment compliance of the patients is at a moderate level and their quality of life is at a good level. The relationship between patients' treatment compliance and their quality of life is also negative.
N36 Inflammatory Bowel Disease Dysplasia Surveillance Pathway (IBD DSP)
Authors: Joseph B , Misra R 
Published: January 2024

Abstract

Background
UK-wide concerns have arisen regarding patients with Inflammatory Bowel Disease (IBD) on surveillance lists being missed or dropped off waiting lists due to the COVID-19 pandemic's impact on waiting times and lists.
An IBD surveillance link nurse role was developed involving clinical sessions with a gastroenterology consultant. The IBD Dysplasia Surveillance pathway (DSP) was created to improve education and counselling for high-risk patients, alleviate concerns, and facilitate shared decision-making in IBD care.

Methods
All patients with dysplasia with a background of IBD are referred to the IBD MDT. After discussion the patients are referred to the DSP clinic. The clinic maintains a dysplasia database capturing all high-risk patients with dysplasia, demographic data, co-morbidities, grade of dysplasia and scheduled endoscopy are collated. Outcome measures of did not attend (DNA) and missed cancers were analysed.

Results
Since April 2022, 170 patients have been referred to the DSP, with 52 reviewed in the surveillance Clinic and 16 referred to joint surgical clinics, with 15 invisible, 34 visible, three high-grade dysplasia and 25 low-grade dysplasia cases. Four patients underwent surgery, and two patients declined surgery. Sixteen patients underwent yearly colonoscopies. Two patients DNA for their colonoscopy. There were no missed cancers.

Conclusion
The IBD Dysplasia Surveillance Pathway is a systematic approach for IBD surveillance. It improves communication between IBD and endoscopy teams. The clinic's hybrid approach improves accessibility and engagement with surveillance, reducing cancellations/DNA of surveillance colonoscopies in the IBD patient population, and facilitating the BSG guidelines for cancer surveillance.