bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimizing Implant Width Selection in Breast Augmentation: Insights From On-Patient Landmark Positioning in 3-Dimensional Breast Simulation
Authors: Akgun Demir Isil 
Published: February 2024

Abstract

Background
The Vectra XT 3D simulation program is a valuable tool for implant selection during the decision-making process regarding breast augmentation. The software allows for the assignment of automatically or manually positioned anatomical landmarks and provides accompanying measurements.

Objectives
This study aimed to present a strategy for standardizing landmark placement on Vectra images during primary breast augmentations, optimizing implant selection and ensuring consistency in breast width data collection.

Methods
The author performed a retrospective analysis of patient data in which breast width measurements were obtained through clinical assessment (CA) and the Vectra program with both automatically (VA) and manually (VM) placed landmarks. Consideration was also given to the thickness of medial and lateral breast tissues. The suggested implant base width from all groups was then compared to the actual implant placed during the surgical procedure.

Results
Comparing the change rates with the actual implant width revealed that rates in CA measurements were significantly lower than those in VA (P &lt; .05), and similarly lower than those in VM (P &lt; .05). Furthermore, change rates in VM were significantly lower than in VA (P &lt; .05). Each group showed a significant positive correlation with the actual implant width.

Conclusions
The Vectra XT 3D simulation program is a valuable adjunct in breast augmentation. However, relying on it solely may lead to inaccuracies in the assessment of breast dimensions due to automatic landmarking. To achieve standardized measurements, landmarks can be marked on the patient before creating the Vectra image. Our results underline the superiority of clinical judgment to simulation programs when establishing a reliable surgical plan.

Level of Evidence: 3
IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis
Authors: Li Wei , Li Hangyu , Hu Qiongyao , Wang Linlin , Yin Zhan , Hu Guangfu 
Published: February 2024

Abstract
Reproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus–pituitary–gonad axis could inhibit the development of gonads. These results indicated that fasting may participate in the regulation of fish gonadal development through the mediation of IGFBP1a. Further studies found that IGFBP1a could markedly inhibit gonadotropin-releasing hormone 3 expressions in hypothalamus cells. At the pituitary level, IGFBP1a could significantly reduce the gonadotropin hormones (LH and FSH) expression by blocking the action of pituitary insulin-like growth factor 1. Interestingly, IGFBP1a could also directly inhibit the expression of lhr, fshr, and sex steroid hormone synthase genes (cyp11a, cyp17a, and cyp19a1) in the ovary. These results indicated that IGFBP1a should be a nutrient deficient response factor that could inhibit fish reproduction through the hypothalamus–pituitary–ovary axis.
Recurrence-free survival as a surrogate endpoint for overall survival after neoadjuvant chemotherapy and surgery for oesophageal squamous cell carcinoma
Authors: Okui Jun , Nagashima Kengo , Matsuda Satoru , Sato Yasunori , Okamura Akihiko , Kawakubo Hirofumi , Muto Manabu , Kakeji Yoshihiro , Kono Koji , Takeuchi Hiroya , Watanabe Masayuki , Doki Yuichiro , Bamba Takeo , Fukuda Takashi , Fujiwara Hitoshi , Sato Shinsuke , Noma Kazuhiro , Miyata Hiroshi , Fujita Takeo , Kitagawa Yuko 
Published: February 2024

Abstract

Background
Overall survival is considered as one of the most important endpoints of treatment efficacy but often requires long follow-up. This study aimed to determine the validity of recurrence-free survival as a surrogate endpoint for overall survival in patients with surgically resectable advanced oesophageal squamous cell carcinoma (OSCC).

Methods
Patients with OSCC who received neoadjuvant cisplatin and 5-fluorouracil, or docetaxel, cisplatin and 5-fluorouracil, at 58 Japanese oesophageal centres certified by the Japan Esophageal Society were reviewed retrospectively. The correlation between recurrence-free and overall survival was assessed using Kendall's τ.

Results
The study included 3154 patients. The 5-year overall and recurrence-free survival rates were 56.6 and 47.7% respectively. The primary analysis revealed a strong correlation between recurrence-free and overall survival (Kendall's τ 0.797, 95% c.i. 0.782 to 0.812) at the individual level. Subgroup analysis showed a positive relationship between a more favourable pathological response to neoadjuvant chemotherapy and a higher τ value. In the meta-regression model, the adjusted R2 value at the institutional level was 100 (95% c.i. 40.2 to 100)%. The surrogate threshold effect was 0.703.

Conclusion
There was a strong correlation between recurrence-free and overall survival in patients with surgically resectable OSCC who underwent neoadjuvant chemotherapy, and this was more pronounced in patients with a better response to neoadjuvant chemotherapy.
Staging of progressive supranuclear palsy-Richardson syndrome using MRI brain charts for the human lifespan
Authors: Planche Vincent , Mansencal Boris , Manjon Jose V , Meissner Wassilios G , Tourdias Thomas , Coupé Pierrick 
Published: February 2024

Abstract
Brain charts for the human lifespan have been recently proposed to build dynamic models of brain anatomy in normal aging and various neurological conditions. They offer new possibilities to quantify neuroanatomical changes from preclinical stages to death, where longitudinal MRI data are not available. In this study, we used brain charts to model the progression of brain atrophy in progressive supranuclear palsy—Richardson syndrome. We combined multiple datasets (n = 8170 quality controlled MRI of healthy subjects from 22 cohorts covering the entire lifespan, and n = 62 MRI of progressive supranuclear palsy—Richardson syndrome patients from the Four Repeat Tauopathy Neuroimaging Initiative (4RTNI)) to extrapolate lifetime volumetric models of healthy and progressive supranuclear palsy—Richardson syndrome brain structures. We then mapped in time and space the sequential divergence between healthy and progressive supranuclear palsy—Richardson syndrome charts. We found six major consecutive stages of atrophy progression: (i) ventral diencephalon (including subthalamic nuclei, substantia nigra, and red nuclei), (ii) pallidum, (iii) brainstem, striatum and amygdala, (iv) thalamus, (v) frontal lobe, and (vi) occipital lobe. The three structures with the most severe atrophy over time were the thalamus, followed by the pallidum and the brainstem. These results match the neuropathological staging of tauopathy progression in progressive supranuclear palsy—Richardson syndrome, where the pathology is supposed to start in the pallido-nigro-luysian system and spreads rostrally via the striatum and the amygdala to the cerebral cortex, and caudally to the brainstem. This study supports the use of brain charts for the human lifespan to study the progression of neurodegenerative diseases, especially in the absence of specific biomarkers as in PSP.
White matter network underlying semantic processing: evidence from stroke patients
Authors: Xiao Xiangyue , Dong Zhicai , Yu Mingyan , Ding Junhua , Zhang Maolin , Cruz Sara , Han Zaizhu , Chen Yan 
Published: February 2024

Abstract
The hub-and-spoke theory of semantic representation fractionates the neural underpinning of semantic knowledge into two essential components: the sensorimotor modality-specific regions and a crucially important semantic hub region. Our previous study in patients with semantic dementia has found that the hub region is located in the left fusiform gyrus. However, because this region is located within the brain damage in patients with semantic dementia, it is not clear whether the semantic deficit is caused by structural damage to the hub region itself or by its disconnection from other brain regions. Stroke patients do not have any damage to the left fusiform gyrus, but exhibit amodal and modality-specific deficits in semantic processing. Therefore, in this study, we validated the semantic hub region from a brain network perspective in 79 stroke patients and explored the white matter connections associated with it. First, we collected data of diffusion-weighted imaging and behavioural performance on general semantic tasks and modality-specific semantic tasks (assessing object knowledge on form, colour, motion, sound, manipulation and function). We then used correlation and regression analyses to examine the association between the nodal degree values of brain regions in the whole-brain structural network and general semantic performance in the stroke patients. The results revealed that the connectivity of the left fusiform gyrus significantly predicted general semantic performance, indicating that this region is the semantic hub. To identify the semantic-relevant connections of the semantic hub, we then correlated the white matter integrity values of each tract connected to the left fusiform gyrus separately with performance on general and modality-specific semantic processing. We found that the hub region accomplished general semantic processing through white matter connections with the left superior temporal pole, middle temporal gyrus, inferior temporal gyrus and hippocampus. The connectivity between the hub region and the left hippocampus, superior temporal pole, middle temporal gyrus, inferior temporal gyrus and parahippocampal gyrus was differentially involved in object form, colour, motion, sound, manipulation and function processing. After statistically removing the effects of potential confounding variables (i.e. whole-brain lesion volume, lesion volume of regions of interest and performance on non-semantic control tasks), the observed effects remained significant. Together, our findings support the role of the left fusiform gyrus as a semantic hub region in stroke patients and reveal its crucial connectivity in the network. This study provides new insights and evidence for the neuroanatomical organization of semantic memory in the human brain.
Isolated right ventricular hypoplasia associated with cyanotic atrial septal defect: a case report
Authors: Gohbara Sakiko , Mochizuki Yasuhide , Fujii Takanari , Tomita Hideshi , Shinke Toshiro 
Published: February 2024

Abstract

Background
Hypoxaemia in isolated right ventricular (RV) hypoplasia (IRVH) is primarily caused by a right-to-left shunt (RLS) at the atrial level, such as an atrial septal defect (ASD). When considering closure of the RLS, it should be closed only after ensuring that it will not cause right-sided heart failure (HF).

Case summary
A 21-year-old woman had been experiencing shortness of breath during exertion since childhood. Transthoracic and transoesophageal echocardiography revealed an ASD with bidirectional shunting, and microbubble test revealed a marked RLS. Cardiac magnetic resonance imaging revealed a hypoplastic RV end-diastolic volume corrected for body surface area of 47 mL/m2 (70% of normal range). Right heart catheterization revealed a decreased Qp/Qs ratio of 0.89 and a pressure waveform with a clear increase in the ‘A’-wave, although the mean right atrial pressure was not high (4 mmHg). Therefore, the patient was diagnosed with cyanotic ASD and IRVH. A temporary balloon occlusion test was performed to evaluate the right-sided heart response to capacitive loading prior to ASD closure. After treatment, the patient’s improved markedly. The pre-operative brain natriuretic peptide (BNP) level was normal; however, 6 months after ASD closure, the BNP level was elevated, and the continuous-wave Doppler waveform of pulmonary regurgitation at the time of transthoracic echocardiography changed, suggesting an increase in diastolic RV pressure.

Discussion
When ASD is complicated by hypoxaemia, the possibility of IRVH, although rare, should be considered. Another difficult point is determining whether the ASD can be closed, considering its immature RV compliance.
A missing coronary guidewire mimicking aortic dissection—a case report
Authors: Arow Ziad , Bar Sef Avigdor , Assali Abid , Arnson Yoav 
Published: February 2024

Abstract

Background
A retained coronary guidewire following coronary angiography is an extremely rare complication. We present a case of a retained coronary guidewire from a percutaneous coronary intervention done 2 years ago.

Case summary
An 80-year-old asymptomatic man with a history of ischemic heart disease and moderate aortic stenosis presented to the echocardiography lab for routine follow-up. Transthoracic echocardiography showed Moderate aortic stenosis and a suspected linear echogenic structure in the ascending aorta. trans-esophageal echocardiography was performed to reveal a mobile and linear echogenic structure originating from the sinuses of Valsalva/Sinotubular junction and extending to the ascending aorta. An electrocardiogram gated cardiac computed tomography was performed and showed A linear well-defined structure originating from the ostium of the left main coronary artery and extending to the ascending aorta—a coronary guidewire from an earlier procedure. A second look at the last invasive coronary angiography record demonstrated the same finding. A multidisciplinary heart team discussion was obtained and concluded that the risk of surgical or endovascular intervention outweighed the potential benefit. The patient was discharged home for a close clinical and echocardiographic follow-up.

Discussion
A retained coronary guidewire is a rare complication that operators should be aware of. Management should be case-specific depending on clinical presentation.
Proteome allocation and the evolution of metabolic cross-feeding
Authors: Labourel Florian J F , Daubin Vincent , Menu Frédéric , Rajon Etienne 
Published: February 2024

Abstract
In a common instance of metabolic cross-feeding (MCF), an organism incompletely metabolizes nutrients and releases metabolites that are used by another to produce energy or building blocks. Why would the former waste edible food, and why does this preferentially occur at specific locations in a metabolic pathway have challenged evolutionary theory for decades. To address these questions, we combine adaptive dynamics with an explicit model of cell metabolism, including enzyme-driven catalysis of metabolic reactions and the cellular constraints acting on the proteome that may incur a cost to expressing all enzymes along a pathway. After pointing out that cells should in principle prioritize upstream reactions when metabolites are restrained inside the cell, we show that the occurrence of permeability-driven MCF is rare and requires that an intermediate metabolite be extremely diffusive. Indeed, only at very high levels of membrane permeability (consistent with those of acetate and glycerol, for instance) and under distinctive sets of parameters should the population diversify and MCF evolve. These results help understand the origins of simple microbial communities, such as those that readily evolve in short-term evolutionary experiments, and may later be extended to investigate how evolution has progressively built up today’s extremely diverse ecosystems.
Evaluating the liver abscess microbiota of beef cattle during a reduction in tylosin supplementation shows differences according to abscess size and fraction
Authors: O'Hara Eóin , Zaheer Rahat , Andrés-Lasheras Sara , McAllister Tim A , Gruninger Robert J 
Published: February 2024

Abstract
Liver abscesses (LA) resulting from bacterial infection in cattle pose a significant global challenge to the beef and dairy industries. Economic losses from liver discounts at slaughter and reduced animal performance drive the need for effective mitigation strategies. Tylosin phosphate supplementation is widely used to reduce LA occurrence, but concerns over antimicrobial overuse emphasize the urgency to explore alternative approaches. Understanding the microbial ecology of LA is crucial to this, and we hypothesized that a reduced timeframe of tylosin delivery would alter LA microbiomes. We conducted 16S rRNA sequencing to assess severe liver abscess bacteriomes in beef cattle supplemented with in-feed tylosin. Our findings revealed that shortening tylosin supplementation did not notably alter microbial communities. Additionally, our findings highlighted the significance of sample processing methods, showing differing communities in bulk purulent material and the capsule-adhered material. Fusobacterium or Bacteroides ASVs dominated LA, alongside probable opportunistic gut pathogens and other microbes. Moreover, we suggest that liver abscess size correlates with microbial community composition. These insights contribute to our understanding of factors impacting liver abscess microbial ecology and will be valuable in identifying antibiotic alternatives. They underscore the importance of exploring varied approaches to address LA while reducing reliance on in-feed antibiotics.
Quantitation of human mitochondrial DNA and whole mtGenomes sequencing of fingernail/hair shaft samples
Authors: Li Hui , Cao Yu , Yang Fan , Liu Xiling , Tao Ruiyang , Xia Ruocheng , Zhu Ruxin , Jiang Lei , Liu Shiquan , Li Chengtao 
Published: February 2024

Abstract
The analysis of mitochondrial DNA (mtDNA) is suitable for fingernail/hair shaft samples in forensic science. A successful mtDNA forensic analysis depends on the quantity and quality of the mtDNA. The application of massively parallel sequence techniques to the analysis of mtDNA has the potential to improve the recovery of genetic information from difficult forensic specimens and to increase the discrimination potential of mtDNA by capturing and comparing full mtGenomes. In this study, we constructed mtDNA-specific standard curves using real-time PCR. The 105-base pair target sequence facilitates the amplification of degraded DNA and is minimally homologous to non-human mtDNA. The results indicated that the assay enables the absolute quantification of down to 10 copies of mtDNA and provides a dynamic range of eight orders of magnitude. Whole mtGenome sequencing experiments demonstrated that as few as 2 000 mtDNA copies resulted in a successful full region amplification and sequencing. Moreover, the frequency of point heteroplasmy from one donor showed that hairs from the same donor have been found to differ within and among themselves and from other tissues, which could impact the interpretation of the results obtained in a forensic investigation.
Deciphering spatial domains from spatially resolved transcriptomics with Siamese graph autoencoder
Authors: Cao Lei , Yang Chao , Hu Luni , Jiang Wenjian , Ren Yating , Xia Tianyi , Xu Mengyang , Ji Yishuai , Li Mei , Xu Xun , Li Yuxiang , Zhang Yong , Fang Shuangsang 
Published: February 2024

Abstract

Background
Cell clustering is a pivotal aspect of spatial transcriptomics (ST) data analysis as it forms the foundation for subsequent data mining. Recent advances in spatial domain identification have leveraged graph neural network (GNN) approaches in conjunction with spatial transcriptomics data. However, such GNN-based methods suffer from representation collapse, wherein all spatial spots are projected onto a singular representation. Consequently, the discriminative capability of individual representation feature is limited, leading to suboptimal clustering performance.

Results
To address this issue, we proposed SGAE, a novel framework for spatial domain identification, incorporating the power of the Siamese graph autoencoder. SGAE mitigates the information correlation at both sample and feature levels, thus improving the representation discrimination. We adapted this framework to ST analysis by constructing a graph based on both gene expression and spatial information. SGAE outperformed alternative methods by its effectiveness in capturing spatial patterns and generating high-quality clusters, as evaluated by the Adjusted Rand Index, Normalized Mutual Information, and Fowlkes–Mallows Index. Moreover, the clustering results derived from SGAE can be further utilized in the identification of 3-dimensional (3D) Drosophila embryonic structure with enhanced accuracy.

Conclusions
Benchmarking results from various ST datasets generated by diverse platforms demonstrate compelling evidence for the effectiveness of SGAE against other ST clustering methods. Specifically, SGAE exhibits potential for extension and application on multislice 3D reconstruction and tissue structure investigation. The source code and a collection of spatial clustering results can be accessed at https://github.com/STOmics/SGAE/.
Joint inversion method of multipoint ambient noise horizontal-to-vertical spectral ratio for 3-D velocity structure of local site and its application
Authors: Wang Jixin , Rong Mianshui , Li Xiaojun , Chen Su , Wang Yushi , Zhu Jun 
Published: February 2024

SUMMARY
The diffusion field theory has been widely used to interpret ambient noise wave fields. Based on this theory, 1-D subsurface velocity structure inversion method is developed. However, few studies have referred to the noise horizontal to vertical (NHV) spectral ratio inversion of 3-D subsurface velocity structures, and almost no effective 3-D NHV inversion tools have been developed. To develop a useful tool for obtaining 3-D soil layer velocity structures, we combined the NHV forward calculation formula derived from diffusion field theory with the guided Monte Carlo algorithm and then extended the single-point NHV inversion to multipoint joint inversion through a joint objective function. Subsequently, a new 3-D soil layer velocity structure inversion method was proposed. Subsequently, a synthetic 2-D case was used to verify the proposed method. Finally, the proposed method was applied to the Xiangtang Array in Tangshan, China, to identify the 3-D velocity structures of the site based on noise observations. The results show that the proposed multipoint joint 3-D inversion method is effective for identifying 3-D underground velocity structures.