bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anatomy, histology and ultrastructure of the digestive tract in Andean fish (Trichomycterus bogotensis) and ecological implications
Authors: Ortiz-Ruiz M. , López-Flórez C. , Castro-Rebolledo M. I. , Baldisserotto B. , Gómez-Ramírez E. 
Published: February 2024

AbstractTrichomycterus bogotensis is one of the three species of fish that inhabit the Colombian Andes. However, several aspects of its basic biology, including its digestive system, are unknown. The morphology and histology of the digestive system are of great importance for understanding a species’ feeding habits and food preferences. Thus, this study aimed to perform a morphological description of the digestive tract of T. bogotensis. The gastrointestinal tract of seven specimens was processed for paraffin histology, high-resolution optical microscopy, and transmission electron microscopy. Anatomically, T. bogotensis exhibits a terminal mouth with villiform teeth, a narrow esophagus, a large and muscular stomach, and a short intestine. Histology and ultrastructure revealed a tissue organization similar to that reported for other Silurids, composed of four layers: mucosal, submucosal, muscular, and serosa. The anatomical and histological characteristics suggest that T. bogotensis is a benthic species with a tendency to carnivory.
Exploring predictors of dysphagia in survivors of head and neck cancer: A cross-sectional study
Authors: López-Fernández María Dolores , Fernández-Lao Carolina , Ruíz-Martínez Alba María , Fernández-Gualda Miguel Ángel , Lozano-Lozano Mario , Ortiz-Comino Lucía , Galiano-Castillo Noelia 
Published: February 2024

Abstract
Purpose
To evaluate the prevalence of dysphagia in survivors of head and neck cancer (sHNC) and to identify the predictors contributing to the development of dysphagia.

Methods
We enrolled 62 sHNC in a cross-sectional study to check the prevalence of dysphagia in sHNC and to evaluate which factors were influencing the presence of this side effect. Besides dysphagia, sociodemographic and clinical characteristics, oral symptoms, maximal mouth opening (MMO), sleep quality and physical condition were evaluated, and a linear regression analysis was performed to verify which of these outcomes impact dysphagia.

Results
Among all the sHNC, 85.5% presented dysphagia. The linear regression analysis confirmed that 44.9% of the variance in dysphagia was determined by coughing, MMO and sleep quality, being MMO the most powerful predictor, followed by coughing and sleep quality.

Conclusion
Dysphagia affected the great majority of sHNC. Moreover, symptoms as coughing, reduced MMO and sleep disorders may act as predictors contributing to the development of dysphagia. Our results emphasize the importance of an early and proper identification of the symptoms as well as an adequate treatment strategy to address the cluster of symptoms that sHNC undergo.
Coverage for evidence-based cancer survivorship care services
Authors: Blaes Anne H. , Abu-Khalaf Maysa M. , Bender Catherine M. , Dent Susan F. , Fung Chunkit , Smith Sophia K. , Watson Samantha , Katta Sweatha , Merrill Janette K. , Hudson Shawna V. 
Published: February 2024

Abstract
Purpose
The American Society of Clinical Oncology Cancer Survivorship Committee established a task force to determine which survivorship care services were being denied by public and private payers for coverage and reimbursement.

Methods
A quantitative survey instrument was developed to determine the clinical practice-reported rates of coverage denials for evidence-based cancer survivorship care services. Additionally, qualitative interviews were conducted to understand whether coverage denials were based on payer policies, cost-sharing, or prior authorization.

Results
Of 122 respondents from 50 states, respondents reported that coverage denials were common (“always,” “most of the time,” or “some of the time”) for maintenance therapies, screening for new primary cancers or cancer recurrence. Respondents reported that denials in coverage for maintenance therapies were highest for immunotherapy (41.74%) and maintenance chemotherapy (40.17%). Coverage denials for new primary cancer screenings were highest for Hodgkin lymphoma survivors needing a PET/CT scan (49.04%) and breast cancer survivors at a high risk of recurrence who needed an MRI (63.46%), respectively. More than half of survey respondents reported denials for symptom management and supportive care services. Fertility services, dental services when indicated, and mental health services were denied “always” or “most of the time” 23.1%, 22.5%, and 12.8%, respectively. Respondents reported they often had a process in place to automatically appeal denials for evidence-based services. The denial process, however, resulted in greater stress for the patient and provider.

Conclusion
Our study demonstrates that additional advocacy with payers is needed to ensure that reimbursement policies are consistent with evidence-based survivorship care services.
Validation of the AO Spine CROST (Clinician Reported Outcome Spine Trauma) in the clinical setting
Authors: Sadiqi Said , de Gendt Erin E. A. , Muijs Sander P. J. , Post Marcel W. M. , Benneker Lorin M. , Holas Martin , Tee Jin W. , Albers Christoph E. , Häckel Sonja , Svac Juraj , Bransford Richard J. , El-Sharkawi Mohammad M. , Kandziora Frank , Rajasekaran Shanmuganathan , Schnake Klaus J. , Vaccaro Alexander R. , Oner F. Cumhur 
Published: February 2024

Abstract
Purpose
To evaluate feasibility, internal consistency, inter-rater reliability, and prospective validity of AO Spine CROST (Clinician Reported Outcome Spine Trauma) in the clinical setting.

Methods
Patients were included from four trauma centers. Two surgeons with substantial amount of experience in spine trauma care were included from each center. Two separate questionnaires were administered at baseline, 6-months and 1-year: one to surgeons (mainly CROST) and another to patients (AO Spine PROST—Patient Reported Outcome Spine Trauma). Descriptive statistics were used to analyze patient characteristics and feasibility, Cronbach’s α for internal consistency. Inter-rater reliability through exact agreement, Kappa statistics and Intraclass Correlation Coefficient (ICC). Prospective analysis, and relationships between CROST and PROST were explored through descriptive statistics and Spearman correlations.

Results
In total, 92 patients were included. CROST showed excellent feasibility results. Internal consistency (α = 0.58–0.70) and reliability (ICC = 0.52 and 0.55) were moderate. Mean total scores between surgeons only differed 0.2–0.9 with exact agreement 48.9–57.6%. Exact agreement per CROST item showed good results (73.9–98.9%). Kappa statistics revealed moderate agreement for most CROST items. In the prospective analysis a trend was only seen when no concerns at all were expressed by the surgeon (CROST = 0), and moderate to strong positive Spearman correlations were found between CROST at baseline and the scores at follow-up (rs = 0.41–0.64). Comparing the CROST with PROST showed no specific association, nor any Spearman correlations (rs = −0.33–0.07).

Conclusions
The AO Spine CROST showed moderate validity in a true clinical setting including patients from the daily clinical practice.
Neurophysiological signatures of mild traumatic brain injury in the acute and subacute phase
Authors: Barone Valentina , de Koning Myrthe E. , van der Horn Harm J. , van der Naalt Joukje , Eertman-Meyer Carin J. , van Putten Michel J. A. M. 
Published: February 2024

Abstract
Background
Mild traumatic brain injury (mTBI) affects 48 million people annually, with up to 30% experiencing long-term complaints such as fatigue, blurred vision, and poor concentration. Assessing neurophysiological features related to visual attention and outcome measures aids in understanding clinical symptoms and prognostication.

Methods
We recorded EEG and eye movements in mTBI patients during a computerized task performed in the acute (< 24 h, TBI-A) and subacute phase (4–6 weeks thereafter). We estimated the posterior dominant rhythm, reaction times (RTs), fixation duration, and event-related potentials (ERPs). Clinical outcome measures were assessed using the Head Injury Symptom Checklist (HISC) and the Extended Glasgow Outcome Scale (GOSE) at 6 months post-injury. Similar analyses were performed in an age-matched control group (measured once). Linear mixed effect modeling was used to examine group differences and temporal changes within the mTBI group.

Results
Twenty-nine patients were included in the acute phase, 30 in the subacute phase, and 19 controls. RTs and fixation duration were longer in mTBI patients compared to controls (p < 0.05), but not between TBI-A and TBI-S (p < 0.05). The frequency of the posterior dominant rhythm was significantly slower in TBI-A (0.6 Hz, p < 0.05) than TBI-S. ERP mean amplitude was significantly lower in mTBI patients than in controls. Neurophysiological features did not significantly relate to clinical outcome measures.

Conclusion
mTBI patients demonstrate impaired processing speed and stimulus evaluation compared to controls, persisting up to 6 weeks after injury. Neurophysiological features in mTBI can assist in determining the extent and temporal progression of recovery.

Graphical abstract
Effect of photodynamic therapy mediated by hematoporphyrin derivatives on small cell lung cancer H446 cells and bronchial epithelial BEAS-2B cells
Authors: Lin Cunzhi , Zhang Yuanyuan , Liao Jiemei , Cui Shichao , Gao Zhe , Han Weizhong 
Published: February 2024

Abstract
To investigate the effects of photodynamic therapy (PDT) mediated by hematoporphyrin derivatives (HPD) on the proliferation of small cell lung cancer H446 cells and bronchial epithelial BEAS-2B cells. H446 cells and BEAS-2B cells were cultured in vitro with different concentrations of HPD(0, 5, 10, 12, 15, 20 μg/mL) for 4 h, and then irradiated with 630 nm laser with different energy densities (0, 25, 50, 75, 100 mW/cm2). Cell viability of H446 cells and BEAS-2B cells were detected by CCK8 assay. The cell apoptosis was observed with Annexin V-FTTC/PI double staining and Hoechst 33258. The RT-PCR examination was applied to detect the transcriptional changes of the mRNA of Bax、Bcl-2, and Caspase-9. The results of CCK8 showed that when the HPD was 15 μg/mL and the laser power density reached 50 mW/cm2, the cell viability was significantly decreased compared with the black control group. Hoechst 33258 staining showed that with the increase of HPD concentration, the cell density was reduced, and apoptotic cells increased. Flow cytometry assay revealed that the apoptotic rates of the HPD-PDT group of H446 cells and BEAS-2B cells were significantly different from those of the blank control group. The RT-PCR examination showed that the expression levels of Bax and Caspase-9 mRNA in the HPD-PDT group were up-regulated, while the expression levels of Bcl-2 mRNA were down-regulated significantly. HPD-PDT can inhibit H446 cells and BEAS-2B cells growth. The mechanism may be related to up-regulating the expression levels of Bax and Caspase-9 mRNA and down-regulating the expression levels of Bcl-2 mRNA.
PCDH8 is a novel prognostic biomarker in thyroid cancer and promotes cell proliferation and viability
Authors: Yang Ruida , Yang Nan , Yin Pan , Xue Zihan , Sun Feidi , Fan Ruihan , Liang JiaFu , Lv Xinru , Wu Shaobo , Sun Liankang 
Published: February 2024

AbstractProtocadherin 8 (PCDH8), a calcium-dependent transmembrane protein in the protocadherin family, regulates cell adhesion and signal transduction. While some studies have provided indirect evidence that PCDH8 has cancer-promoting properties, this association is controversial. In particular, its involvement in thyroid cancer (THCA) remains unclear. We aimed to elucidate the role of PCDH8 in THCA using bioinformatic analysis. Subsequently, the results were experimentally validated. The analysis conducted using the R programming language and online web tools explored PCDH8 expression levels, prognostic, and clinical implications, and its relationship with the tumor immune microenvironment in THCA. Furthermore, we examined the association between PCDH8 and co-expressed genes, highlighting their involvement in several biological processes relevant to THCA. The potential of PCDH8 as a therapeutic target for this pathology was also explored. Immunohistochemical (IHC) staining was performed on samples from 98 patients with THCA, and experimental validation was carried out. PCDH8 was significantly elevated in cancer tissues and associated with poor prognosis, several clinical factors, and immune cell and checkpoint abundance. Cox regression and survival analyses, together with Receiver Operating Curves (ROC) indicated that PCDH8 was an independent prognostic factor for THCA. Furthermore, PCDH8 impacts cell viability and proliferation, promoting tumorigenesis. Also, it influences tumor cell sensitivity to various drugs. Thus, PCDH8 might be a potential therapeutic target for THCA. IHC, cell culture, MTT, and colony formation experiments further confirmed our findings. This analysis provided insights into the potential carcinogenic role of PCDH8 in THCA, as it impacts cell viability and proliferation. Thus, PCDH8 might play an important role in its prognosis, immune infiltration, and diagnosis.
Treatment strategies in MDA5-positive clinically amyopathic dermatomyositis: a single-center retrospective analysis
Authors: Hirsch Stefanie , Pöhler Gesa Helen , Seeliger Benjamin , Prasse Antje , Witte Torsten , Thiele Thea 
Published: February 2024

AbstractMelanoma differentiation-associated protein 5 (MDA5) antibody positive amyopathic dermatomyositis (DM) is a rare inflammatory disease. So far, there is no official treatment guideline in MDA5 amyopathic dermatomyositis, but early and aggressive immunosuppressive combination treatment can induce a stable remission. We retrospectively analyzed a cohort of eight patients (male n = 5) that were diagnosed with MDA5-positive amyopathic DM. Patient data comprised demographics, CT-guided diagnosis of pulmonary involvement, pulmonary function testing including forced vital capacity (FVC) and diffusing capacity for carbon monoxide (DLCO) data on baseline and mean long-term follow-up of 51 months (24–92 months) to evaluate treatment strategies. Depending on severity of organ involvement treatments were individualized including cyclophosphamide, immunoglobulins and plasmapheresis. Simultaneously, oral treatment with tacrolimus was commenced in four of the eight patients. Most patients received remission maintenance therapy with a combination of tacrolimus, rituximab and low dose steroids. In all patients, improvement in FVC was recorded and five patients achieved an improvement in DLCO. An improvement in the CT imaging morphological findings was observed in four patients. Awareness for the entirety of all clinical and disease-related findings of amyopathic DM is crucial, and remission maintenance is often achieved with a combination of tacrolimus and rituximab.
Overexpression of thioredoxin-like protein ACHT2 leads to negative feedback control of photosynthesis in Arabidopsis thaliana
Authors: Fukushi Yuka , Yokochi Yuichi , Hisabori Toru , Yoshida Keisuke 
Published: February 2024

AbstractThioredoxin (Trx) is a small redox mediator protein involved in the regulation of various chloroplast functions by modulating the redox state of Trx target proteins in ever-changing light environments. Using reducing equivalents produced by the photosynthetic electron transport chain, Trx reduces the disulfide bonds on target proteins and generally turns on their activities. While the details of the protein-reduction mechanism by Trx have been well investigated, the oxidation mechanism that counteracts it has long been unclear. We have recently demonstrated that Trx-like proteins such as Trx-like2 and atypical Cys His-rich Trx (ACHT) can function as protein oxidation factors in chloroplasts. Our latest study on transgenic Arabidopsis plants indicated that the ACHT isoform ACHT2 is involved in regulating the thermal dissipation of light energy. To understand the role of ACHT2 in vivo, we characterized phenotypic changes specifically caused by ACHT2 overexpression in Arabidopsis. ACHT2-overexpressing plants showed growth defects, especially under high light conditions. This growth phenotype was accompanied with the impaired reductive activation of Calvin–Benson cycle enzymes, enhanced thermal dissipation of light energy, and decreased photosystem II activity. Overall, ACHT2 overexpression promoted protein oxidation that led to the inadequate activation of Calvin–Benson cycle enzymes in light and consequently induced negative feedback control of the photosynthetic electron transport chain. This study highlights the importance of the balance between protein reduction and oxidation in chloroplasts for optimal photosynthetic performance and plant growth.
Human presence is positively related to the number of bird calls and songs: Assessment in a national park
Authors: Pérez-González Javier , Rey-Gozalo Guillermo , Hidalgo-de-Trucios Sebastián J. 
Published: February 2024

AbstractHuman disturbance has been shown to provoke physiological and behavioral responses in birds, so nature-based tourism might reduce bird abundance and diversity. The negative consequences of human disturbance might be expected to be maximized during eventual massive events in highly protected areas such as national parks. In this study, the consequences for soundscapes of human presence and disturbance of thousands of visitors during an ornithological fair (massive event) on the bird community of the Monfragüe National Park (Spain) were analyzed. We found that the number and diversity of bird vocalizations did not decrease during the massive event. In contrast, the presence of people in the Monfragüe National Park was associated with an increase in the number and diversity of vocalizations. The effect of human presence on the number of calls and songs differed: the number of calls mainly increased during the massive event when people were present, while the number of songs increased when people were present, particularly during the measurement campaign without the massive event. The human shield hypothesis, along with other behavioral and environmental factors, might potentially explain the results obtained.
Subgraphs of BV functions on RCD spaces
Authors: Antonelli Gioacchino , Brena Camillo , Pasqualetto Enrico 
Published: February 2024

AbstractIn this work, we extend classical results for subgraphs of functions of bounded variation in $$\mathbb R^n\times \mathbb R$$

R
n

×
R

to the setting of $${\textsf{X}}\times \mathbb R$$

X
×
R

, where $${\textsf{X}}$$
X
is an $${\textrm{RCD}}(K,N)$$

RCD
(
K
,
N
)

metric measure space. In particular, we give the precise expression of the push-forward onto $${\textsf{X}}$$
X
of the perimeter measure of the subgraph in $${\textsf{X}}\times \mathbb R$$

X
×
R

of a $${\textrm{BV}}$$
BV
function on $${\textsf{X}}$$
X
. Moreover, in properly chosen good coordinates, we write the precise expression of the normal to the boundary of the subgraph of a $${\textrm{BV}}$$
BV
function f with respect to the polar vector of f, and we prove change-of-variable formulas.
Wasserstein GAN-based architecture to generate collaborative filtering synthetic datasets
Authors: Bobadilla Jesús , Gutiérrez Abraham 
Published: February 2024

AbstractCurrently, generative applications are reshaping different fields, such as art, computer vision, speech processing, and natural language. The computer science personalization area is increasingly relevant since large companies such as Spotify, Netflix, TripAdvisor, Amazon, and Google use recommender systems. Then, it is rational to expect that generative learning will increasingly be used to improve current recommender systems. In this paper, a method is proposed to generate synthetic recommender system datasets that can be used to test the recommendation performance and accuracy of a company on different simulated scenarios, such as large increases in their dataset sizes, number of users, or number of items. Specifically, an improvement in the state-of-the-art method is proposed by applying the Wasserstein concept to the generative adversarial network for recommender systems (GANRS) seminal method to generate synthetic datasets. The results show that our proposed method reduces the mode collapse, increases the sizes of the synthetic datasets, improves their ratings distributions, and maintains the potential to choose the desired number of users, number of items, and starting size of the dataset. Both the baseline GANRS and the proposed Wasserstein-based WGANRS deep learning architectures generate fake profiles from dense, short, and continuous embeddings in the latent space instead of the sparse, large, and discrete raw samples that previous GAN models used as a source. To enable reproducibility, the Python and Keras codes are provided in open repositories along with the synthetic datasets generated to test the proposed architecture (https://github.com/jesusbobadilla/ganrs.git).
Graphical abstract
Caring records: professional insights into child-centered case note recording
Authors: Hawkes Martine , Evans Joanne , Reed Barbara 
Published: February 2024

AbstractThe consequences of poorly processed reports of child abuse and neglect, along with governance challenges in child protection systems, are well-documented. Recent research, inquiries and royal commissions emphasise the need for child-centered and participatory practices that support the rights and dignity of children and their families. However, the challenges of quality case recording in child protection systems and contexts remain unclear. This paper reports on the findings from a pilot study that interviewed (n = 22) and surveyed (n = 56) social work students and social work curriculum developers from Australian Universities and practitioners currently working in the Australian child protection service system. By capturing participants' professional insights, we aim to understand the embedded barriers to transforming child-centered systems by focusing on strengths and possibilities in current practices rather than reiterating deficiencies in recordkeeping. This paper reveals insights into how professionals working in the child protection system understand and are supported in child-centered case note recording and recordkeeping practices. It also identifies the possibilities for the crucial role that interdisciplinary collaboration and alignment between social work and recordkeeping informatics can play in transforming and supporting recordkeeping approaches and practices that prioritise and uphold the rights and dignity of the child.