bluuebunny/crossref_metadata_2025_split huggingface dataset:
#176 : The Relationship Between Blastocyst Morphology and PGD Result
Authors: Saidatuzzahroh Nur 
Published: January 2024

Background and Aims: Blastocyst morphology has been clinically assessed during the selection of blastocyst for transfer and cryopreservation, and the morphological grading scheme has gained wide acceptance. Blastocyst grading according to Gardner et al. (20) early blastocyst, blastocoel less than half of the blastocyst (1AA), blastocyst with blastocoel more than half of the blastocyst (2AA), blastocyst with blastocoel fill the blastocyst (3AA), and Expanded Blastocyst (4AA/4BB/4CC). Morphology blastocyst with good quality determining the success of PGD. Preimplantation genetic diagnosis (PGD) are routine procedures performed in many in vitro fertilization (IVF) clinics. In patients with genetic or chromosomal abnormalities, PGD is an integral part of the IVF program. This study aims to determine whether embryos with normal blastocyst morphology have euploidy result on the PGD test. Method: Observational research study. Results: Dari 22 embryo blastocyst yang di PGD, 59% embryo euploidy 4AA/4BB, 22,7% embryo low level mosaicism 4AA/4BB, 13% embryo aneuploidy 2AA/3AA and 4,5% embryo multiple aneuploidy 3AA. The result suggested that the euploidy rate of blastocyst grade 4AA was the highest. Conclusion: Based on data, embryo morphology with quality 4AA/4BB show PGD result Euploidy was the highest. Euploidy of embryo is one of the important factors affecting embryo implantation and development. Euploidy of blastocyst during PGD treatment was found to be correlated with morphological assessment (Gardner grade)
#281 : IVF Outcome of Good Prognosis Patients Based on Sperm Morphology Grading Using Intracytoplasmic Morphologically Selected Sperm Injection (IMSI) Technique
Authors: Aziz Arif Sofyan , Boediono Arief , Rizal Muhammad , Indrasari Wahyu , Rahmawati Pitra , Szeifoul  , Sini Ivan R 
Published: January 2024

Background and Aims: Spermatozoa morphology is one of the factors that can influence the success of IVF. IMSI is a technique that provides various selection criteria of sperm head’s morphology and the presence of vacuoles with a total magnification of over 6,000. The purpose of this study was to compare the embryo development of good prognosis patients based on sperm morphology grading using IMSI technique. Method: This study was a retrospective analysis performed at Morula IVF Jakarta from 2019 to 2022 based on good prognosis patients (age [Formula: see text] 38 years, AFC [Formula: see text] 7, AMH [Formula: see text] 1.1). There were two groups in this study, (A) sperm grade I and II, (B) sperm grade III and IV, based on Vanderzwalden et al. (2008). The number of patients in group (A) was 1035 patients, and group (B) was 345 patients. The parameters observed in this study were fertilization rate (FR), top embryo quality rate on day 3 (D3) and day 5 (D5), blastocyst rate (BR). Results: The result study showed that (FR) were significantly increased (P=0.000) in group (A) compared to group (B), which was 77.28% and 72.90%. In addition, group (A) had significantly (D3), which was 51.37% (P=0.012) compared to group (B) 46.92%. On the other hand (D5) and (BR) were significantly increased (P=0.000) in group (A), by about 42.58% and 48.01%, contrasted to group (B), which was only 36.11% and 39.21%. Conclusion: Grade I and II sperm of IMSI technique significantly increased IVF outcome in good prognosis patient.
#297 : The Impact of Large Perivitelline Space and Fragmented Polar Body Oocytes on the Quality of Embryos and Pregnancy Loss in IVF Program
Authors: Rizki Syifa Ghufrani , Widyastuti Rini , Gunarwati Deka Putri , Al-Anshori Akhmad Afifudin , Dinanty Diar Trie , Nuraisah Gita Salmah , Khaerulloh Muhammad , Riyanti Aida , Prodjokusumo Fachry Achmad , Wiweko Budi 
Published: January 2024

Background and Aims: Extracytoplasmic oocyte abnormalities such as Large Perivitelline Space (LPVS) and Fragmented Polar Body (FrPB) are frequently detected in human oocytes during IVF procedures. The purpose of this research is to determine the relationship of LPVS, FrPB, and their combination on embryo quality and pregnancy loss. Method: The 36 positive pregnancy cases from under 40 years old patients that underwent ICSI using normospermia at RS Pondok Indah IVF Centre were observed. Patients were stimulated with r-FSH utilizing an antagonist protocol for 10-11 days, then r-HCG was administered as a trigger 36 hours before retrieval. Oocytes were classified as follows: (a) normal, (b) large perivitelline space (LPVS), (c) polar body fragmentation (FrPB), and (d) a combination of LPVS and FrPB. The Shapiro-Wilk and Kruskal-Wallis tests were used to determine the effect between each of the categories of oocyte with D3 and D5 embryo quality, along with pregnancy loss. Results: The results indicate that there was no significant distinction among the groups in terms of embryo quality on D3 and D5, as well as pregnancy loss (p=0.187, 0.881, and 0.561, respectively). Conclusion: It could have been concluded that LPVS, FrPB, and their combination have no impact on embryo quality and pregnancy loss.
#32 : Female Genital Tuberculosis with Primary Subfertility — A Case Report
Authors: Farooq Fariha 
Published: January 2024

Background and Aims: Tuberculosis is a major health problem with worldwide incidence of about 10.4 million people TB in 2015, out of which more than 60% were in Asia. There is no single diagnostic test for female genital tuberculosis so diagnosis is made after considering various factors such as family history, general physical examination, culture and PCR tests and also the diagnostic hysteroscopy and laparoscopy. Here we report a case of asymptomatic female genital tuberculosis in which diagnosis was made on the basis of laparoscopic findings as well as histopathology. Method: Patient 30 years with no symptom, planned to have laparoscopy and proceed as workup for primary subfertility and ultrasound showing 44 * 22 mm cyst with internal echoes in right adnexal region, with impression of right endometriotic cyst. Results: Laparoscopy showed normal uterus. There was 0.5 * 0.6 cm granuloma on surface fundus of uterus, adhesions in Pouch of Doughlas. Right tube had gross hydrosalpinx with fimbrial phymosis, and golf stick appearance. Right ovary was buried under right tube and had cyst of size 4 * 5 cm, on drainage caseous material. Left tube had moderate hydrosalpinx and left ovary was completely buried in adhesions. No fill and spill of dye on dyes test. Adhesiolysis was done and anatomy restored. Right tubo ovarian mass drained and caseous material removed. Uterine fundal caseous material removed and collected for histopathology which confirmed the diagnosis. Giant cell with granuloma and caseous necrosis was confirmed on histopathology. Conclusion: Female genital tuberculosis is associated with late presentation due to asymptomatic nature of disease. Hysteroscopy and laparoscopy with microbiological tests on samples taken during laparoscopy has made the diagnosis near to confirmatory. Correct and complete medical treatment gives good results.
#149 : Improved Embryo Quality of Infertile Women at Different Ages by Oral Administration of an Antioxidant Mixture
Authors: Takahashi Yuji , Qin Junwen , Akasaki Masayoshi 
Published: January 2024

Background and Aims: Oxidative stress is known to affect oocyte/embryo quality. In the previous meeting of ASPIRE, an antioxidant mixture (AOM) developed by us was shown to improve oocyte/embryo quality. Since oxidative stress is related to aging of body, AOM may be able to support the oocyte/embryo quality of women at advanced ages. We therefore examined the effects of AOM administration on the oocyte/embryo quality of women at different ages. Method: AOM supplement was given orally for at least one and half month (at most 7 months) to infertile women who had participated in at least one IVF/ICSI program and whose quality of embryos was poor or fair. After the supplementation period, oocyte-pickup was carried out, and either IVF or ICSI was subjected to the patients. Then, the data of oocyte maturity, fertilization, developmental capacity, and bioavailability of embryos (No. of embryos transferred and cryopreserved) were collected retrospectively. Results: The effects of AOM on the embryo quality of women at different ages were compared (Age groups were: [Formula: see text] 30yr; 31 [Formula: see text] 35yr; 36 [Formula: see text] 40yr; 41yr[Formula: see text]). In all groups, oocyte retrieval and maturation rates were slightly increased. More evidently, the developmental capacity, especially blastocyst formation, was significantly improved by AOM in all groups ([Formula: see text] 30yr: 20.0% vs 63.6%; 31 [Formula: see text] 35yr; 30.8% vs 68.9%; 36 [Formula: see text] 40yr: 36.4% vs 60.8%; 41yr[Formula: see text]: 36.4% vs 52.9%). Accordingly, the number of embryos cryopreserved was markedly increased ([Formula: see text] 30yr: 0.3±0.6 vs 1.8±2.4; 31 [Formula: see text] 35yr; 0.7±0.9 vs 1.4±1.2; 36 [Formula: see text] 40yr: 0.6±0.8 vs 1.1±1.0; 41yr[Formula: see text]: 0.0±0.0 vs 0.6±0.9), indicating that embryo quality in all groups was improved. Conclusion: Although it was more beneficial for younger women, AOM administration was very effective to improve oocyte/embryo quality in all age groups. Our present results strongly suggest that AOM can ameliorate age-dependent decrease of embryo quality.
#29 : A Retrospective Review of Patients Undergoing Fertility Preservation in a Tertiary Institution in Singapore Over 10 Years
Authors: Goh Charissa , Ee Tat Xin , Viardot-Foucault Veronique , Phoon Jessie Wai Leng 
Published: January 2024

Background and Aims: Early detection and advancement in cancer treatments have drastically improved survivorship. Hence, there is a shift towards long-term quality of life goals such as protecting the oncologic patient’s fertility potential. The study aims to determine the uptake and use of fertility preservation options in women presenting at a tertiary institution in Singapore. This is the first review to outline the uptake rates, methods and fertility outcomes among women undergoing fertility preservation in Singapore. Method: The study is a retrospective review of premenopausal female patients presenting to a single tertiary institution between 2010 and 2019 for fertility preservation. Our primary outcome measures are the uptake rate, type of fertility preservation undertaken and live birth rate. Our secondary outcome are reasons for decline of fertility preservation. Results: Forty-one patients were referred between 2010 and 2019. There were 2 referrals received in 2010 compared to 10 referrals in 2019. Twenty-nine patients underwent fertility preservation procedures, with 27 (93.1%) successful fertility preservation procedures performed. Most of the patients opted for embryo cryopreservation (44.4%) or oocyte cryopreservation (33.3%). Three patients (11.1%) underwent ovarian tissue cryopreservation. Two patients (7.4%) opted for a combination of oocyte and embryo cryopreservation and 1 patient (3.7%) had a combination of ovarian tissue and oocyte cryopreservation. There were 3 successful live births. One patient achieved a live birth from stored embryos achieved from in-vitro maturation subsequent fertilisation. The other two patients achieved live births from stored embryos and oocytes respectively. The most commonly cited reason for declining fertility preservation was financial limitations (41.7%). Conclusion: While the referral rates have been increasing over the years, the rates remain low. Further studies can be done to assess the barriers to fertility preservation referrals. Funding for fertility preservation procedures may increase uptake in this group of women.
#205 : Pre-Orchidectomy Fertility Preservation in a Regional Queensland Centre - Patient Attitudes to Sperm Banking
Authors: Stehr Emma , Desai Devang , Ravichandran Kapilan 
Published: January 2024

Background and Aims: Fertility preservation for testicular cancer remains an essential part of the pre-operative radical orchidectomy discussion. In Australia, testicular cancer is estimated to account for 1.1% of all new male cancer diagnoses, with greater than 800 new cases a year diagnosed in Australia. Toowoomba Hospital and its urology service covers a large geographical area, extending from Toowoomba to the northern territory border. This poses both an access issue for both urological review and subsequent fertility interventions. Despite geographical barriers, pre-operative sperm banking (cryopreservation) remains generally recommended prior to operative intervention. The aim of this study was to investigate adherence to guidelines and identification of barriers or reasons for patients not wanting to engage with fertility preservation. Method: A retrospective analysis of patients undergoing a radical inguinal orchidectomy for the purposes of testicular cancer. Patient records were examined for discussion of fertility preservation and patient outcomes for attending a fertility-consultation. Results: Between 2016-2022, 54 patients underwent a radical orchidectomy at the Toowoomba Hospital. All patients pre-operatively had discussion surrounding fertility preservation and where patient accepted, referrals were made to the closest fertility provider. The majority of patients who refused pre-operative sperm banking had clear reasoning including completion of family. Conclusion: Fertility preservation discussions were part of all interactions pre-operatively where appropriate. Patient reasoning for declining preservation services were varied.
#58 : Relationship Between E-Cigarettes (Vaping) Habits and Semen Parameters Among Patient Attending Sabahcare Fertility Centre
Authors: Chiang Jessy Mee Voon , Masri Mohd Taufeeq , Chin Michelle Jane , Yunos Nur Asyiqin Binti , Cheong Yee Tsing , Hatta Tarmizi M. 
Published: January 2024

Background and Aims: Exposure to nicotine is known to reduce sperm count and motility as well as increasing abnormal parameters. This study aimed to determine the differences of Seminal Fluid Analysis (SFA) between e-cigarettes users (vaping) and non-vaping patients attending SabahCare Fertility Centre. This study is still on-going, more samples are to be expected before the 12[Formula: see text] Congress of ASPIRE Method: Data is collected by handing out questionnaire to male patient doing SFA in the centre. Samples are examined according to WHO laboratory manual for the examination and processing of human semen, sixth edition (WHO, 2021) criteria. Sperm parameters we applied are normozoospermia, oligozoospermia (sperm concentration <14 × 106/ml), azoospermia (no spermatozoa in the ejaculate), asthenozoospermia (sperm motility and/or progressive motility are below the reference value). Chi-Square test analysis will be performed to determine the significance level of the results. Results: Currently, a total of 38 sample sizes are collected. 60.5% are in the normal SFA quality and 39.5% are in the abnormal SFA quality. Conclusion: Sample collection to determine if using e-cigarettes (vaping) affects semen quality among SabahCare Fertility Centre are still ongoing.
#157 : The Effect of COVID-19 Infection on Sperm DNA Fragmentation Index and Pregnancy Outcome in Teratai IVF Clinic Jakarta
Authors: Marthasari Rossy , Yan Irsal , Glamour Seindy , Harijanto Harijanto , Ronggani Andrie 
Published: January 2024

Background and Aims: Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS CoV 2) has highly suspected as one of the causes of male infertility. Much research has been showed that sperm parameters decreased after Covid-19 infection. But there is limited data that show whether Covid-19 has impact on Sperm DNA Fragmentation Index (DFI) and pregnancy outcome. The aim of this study is to assess the effect of Covid-19 infection and DFI in male and pregnancy outcome. Methods: Thirty-four males who came to Teratai clinic as patients were selected and divided into two groups: Covid positive and Covid negative. The criteria to be included in Covid positive is the fact that the patient recently has Covid infection for less than 6 months. We assessed DFI outcome and divided the data into two group: Poor DFI (>25%) and Normal DFI (<25%). We also analyzed pregnancy outcome and divided the data into two groups: pregnancy positive and pregnancy negative. Statistical analysis is performed by Chi-square. Results: Result demonstrated that Covid-19 infection increased Sperm DFI (p<0,05) and decreased pregnancy outcome (p<0,05). Conclusion: Covid-19 infection has a negative influence on sperm DFI and pregnancy outcome.
#369 : A Case of Luteal Phase Deficiency (LPD) and Polycystic Ovarian Syndrome (PCOS). When Routine is not Enough
Authors: Chua Ai Chen , Tham Seong Wai 
Published: January 2024

Background and Aims: Luteal phase deficiency (LPD) is a consequence of the inability of the corpus luteum (CL) to produce an adequate level of progesterone. This manifests clinically as a short menstrual cycle and infertility. Abnormal follicular development and inadequate steroidogenesis in lutein cells of the CL have been implicated in CL dysfunction and LPD. LPD and PCOS are independent disorders sharing a common pathophysiology. The aim of this case report is to highlight the importance of additional luteal phase support for patients with PCOS. Case report: A 34-year-old Indian lady with anovulation secondary to PCOS failed to get pregnant after 7 years of marriage. Her menses were irregular, and she had hirsutism. She was also overweight. (BMI 26.4 kg/m2) Her hormonal profile showed a reversal of the FSH/LH ratio of 2:1, and serum progesterone was low. (<1.6 IU/L). A transvaginal scan showed a classic pearl string sign, which was typical for patients with PCOS. She underwent laparoscopic dye insufflation and ovarian drilling, during which four puncture points were made on each ovary at 4 watts for 4 seconds. Later, she underwent a round of timed sexual intercourse and intrauterine insemination. (IUI) Dydrogesterone (10 mg, 8 hourly) was started for luteal phase support as per unit protocol. However, she complained of per vaginal spotting starting on day 7 after human chorionic gonadotropin (hcg) trigger, and both times, treatment was unsuccessful. Then, she had another round of ovarian stimulation with clomiphene citrate 50 mg daily and had IUI. She was given micronized progesterone 100 mg 12 hours a day in addition to the dydrogesterone. The urine pregnancy test was positive, and she is having a healthy, on-going pregnancy. Conclusion: Patients with PCOS may suffer from a defective luteal phase. Hence, it is important to supplement PCOS patients with additional support during the luteal phase, as this can lead to a successful pregnancy.
#347 : Factors Related to Mosaicism of Human Embryo: Conventional Statistics and Machine Learning Analysis
Authors: Tran Huy-Phuong , Nguyen Long Nu-Hai , Ho Tuong-Nguyen , Do Vy Nguyen-Thao , Ly Loc-Thai , Hoang Tuyet Thi-Diem 
Published: January 2024

Background and Aims: Mosaicism arises from errors in the cell division process and can lead to cells containing a combination of normal and mutated genes. Mosaic embryos can produce healthy babies. However, it may be associated with a lower implantation and a higher miscarriage rate. Despite extensive research, the scientific understanding of the underlying causes of embryonic mosaicism remains limited, and conclusions remain subject to ongoing debate. Machine learning (ML) can capture complex relationships and automatically learn the most informative features, while conventional statistics (CS) are simple to understand. We aimed to apply both ML and CS to determine the critical factors that may be associated with mosaicism. Method: A retrospective analysis was conducted at the Infertility Department of Hung Vuong Hospital between 2018 and 2022 using medical data from 51 IVF couples. The study utilized both machine learning and conventional statistical methods to identify possible factors associated with mosaicism in human embryos. Results: Out of the 246 embryos studied, 84 (32%) were found to be mosaic. The performance of the classifiers in the machine learning analysis was relatively poor when using all features, with F1-scores ranging from 0.08 to 0.26. While feature selection helped to improve performance, the F1-scores remained low. On the other hand, the conventional statistical analysis revealed that patients with cryptozoospermia showed a positive association with mosaicism (OR 2.68, 95% CI 1.08 to 6.66, p=0.033). Conclusion: According to the machine learning algorithms, the current set of clinical features has no relation to the incidence of mosaicism. However, the conventional statistical analysis suggests that patients with cryptozoospermia may contribute to a higher risk of mosaicism. Further research is needed to fully understand the mechanisms underlying mosaicism in human embryos.
#209 : The Association Between Autoimmune Diseases and Gestational Diabetes Mellitus: A Mendelian Randomization Study
Authors: Ou Taoaixin 
Published: January 2024

Background and Aims: As a common complication of pregnancy, gestational diabetes mellitus (GDM) may lead to a serious negative impact on pregnant women and their children. Autoimmune diseases are chronic pathologies triggered by abnormal responses of the human immune system that result in damage and disorders in the body. Recently, several studies have demonstrated that autoimmune diseases could be related to the risk of GDM. However, several other studies of this relationship have yielded conflicting results. We explored the causal relationship between autoimmune diseases and GDM risk through a two-sample Mendelian randomization (2SMR) study. Method: We obtained exposure data for rheumatoid arthritis (RA) from a genome-wide association study (GWAS) pooled dataset, which consisted of 361,194 participants. We selected single-nucleotide polymorphisms (SNPs) that had a relationship with RA and were at a significance level of not only p < 5 × 10[Formula: see text] but also at a low linkage disequilibrium (LD) level (r2 < 0.01). The results included 5,687 GDM cases and 117,892 controls. For detailed Methods, please see the Methods section in this article’s Online Repository at https://www.jacionline.org Results: The results of inverse variance weight (IVW) analysis suggested a positive correlation between RA and GDM risk. In addition, no validity was observed in the statistical models for heterogeneity and pleiotropy. However, for multiple sclerosis (MS), no causal relationship between it and GDM risk was found in our study. Conclusion: Our results suggest a strong casual association between RA and the risk of GDM. The results suggested the importance of enhanced antenatal care and early intervention among pregnant women with RA as well as clues for novel risk factors for GDM.