bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climatic response of budburst in the mountain birch at two areas in northern Fennoscandia and possible responses to global change
Authors: Karlsson P. S. , Bylund H. , Neuvonen S. , Heino S. , Tjus M. 
Published: October 2003

The relationship between the climate and budburst of the mountain birch was evaluated for two areas in subarctic (ca 69°N) Fennoscandia; at Abisko, Swedish Lapland, and at Kevo, Finnish Lapland. Thermal time (TT, degree‐day sums) to budburst was calculated for experimental conditions in the laboratory and for in situ observations of budburst. Two types of models predicting leaf emergence in situ were used: 1) TT to budburst for different threshold temperatures based on daily mean (TTMean) or daily maximum (TTMax) temperatures and 2) ecophysiological budburst models. The obtained models were used to estimate effects of a changed climate.

Laboratory experiments of TT to budburst indicated no differences in the thermal requirements at the two areas. Temperature requirements of budburst declined successively during the progression of spring, from ca 250 degree‐days (>+2°C) in January to ca 100 in May. No significant trend in the date of budburst was found over the last 70 (Abisko) or 20 (Kevo) yr. There were some differences in the type of model that best explained the date of budburst in situ at the two areas. For Kevo the best prediction (minimum root mean square error, RMSE) of budburst was obtained by a simple thermal time model (TTMean>5.5°C) from 1 January (RMSE=2.1). For budburst at Abisko, models based on daily maximum temperature fitted better than those based on daily means. For Abisko, models based on thermal time accumulation only showed systematic errors in the predicted budburst that were correlated with budburst previous year (BBPY). Including this apparent memory effect in the model decreased the error by 2.4 d. The best prediction for Abisko was thus obtained using TTMAX>6.5 (RMSE=3.1) from 1 January.  Using these models to predict the effect of a changed air temperature climate indicate 3–8 d earlier budburst for a one‐degree increase in temperature, the effect being smaller for Kevo than for Abisko. For both areas a change in May temperature has a larger effect on the date of budburst than changes in any other month.
Effects of anoxia on megakaryocyte progenitors derived from cord blood CD34pos cells
Authors: Saxonhouse Matthew A , Rimsza Lisa M , Christensen Robert D , Hutson Alan D , Stegner Joseph , Koenig Joyce M , Sola Martha C 
Published: October 2003

Abstract: Background: Severe hypoxic insults to the fetus and neonate are associated with the development of thrombocytopenia. The thrombocytopenia in some cases is the result of disseminated intravascular coagulation, but that mechanism fails to account for all, perhaps the majority, of cases.Objective: We hypothesized that human fetal megakaryocyte (Mk) progenitors are directly adversely affected by transient anoxia.Design and methods: To test this, we isolated CD34pos cells from the umbilical cord blood of 10 healthy term neonates, and exposed these to 0% or 20% O2 for 24 h, with or without recombinant thrombopoietin (rTpo, 50 ng/mL). After 24 h, a portion of the CD34pos cells were harvested for flow cytometric evaluation of apoptosis. The remaining cells were cultured for an additional 10–12 days, under normoxic conditions, in a collagen‐based serum‐free system containing rTpo, IL‐3, and IL‐6. In this way, we sought to determine the effect of transient anoxia on clonogenic capacity of Mk progenitors.Results: Contrary to our hypothesis, anoxia did not increase either apoptosis or cell death of the CD34pos cells. The addition of rTpo was protective, with a significant decrease in apoptosis and cell death (P < 0.0001), and an increase in the number of Mk colonies cultured (P = 0.04). There was no difference between the normoxic and anoxic groups in proliferative potential of the Mk progenitor cells.Conclusions: The thrombocytopenia observed in neonates following an acute hypoxic event is not likely due to a direct deleterious effect of hypoxia on Mk progenitors.
Prevalence of oral mucosal lesions in elderly people in Santiago, Chile
Authors: Espinoza I. , Rojas R. , Aranda W. , Gamonal J. 
Published: October 2003

AbstractBackground:  Oral prevalence studies are important to know the state of health and the needs of treatment. Our aim was to determine the prevalence of oral mucosal lesions and associated factors among aging Chileans.Methods:  A random sample by age, gender, and socioeconomic status was obtained, comprising 889 individuals older than 65 years. Individuals were interviewed and examined in Santiago, the capital of Chile, according to the World Health Organization guidelines.Results:  The prevalence of one or more oral mucosal lesions in the sample was 53%. Logistic regression model revealed that denture use increased the probability of one or more oral mucosal lesions by threefold, while age, gender, smoking, medication use, xerostomia, and social or cultural factors had no effect. The most common lesion was denture stomatitis (22.3%), followed by irritative hyperplasia (9.4%), oral mucosal varicosities (9%), solitary pigmented lesions (4%), traumatic ulcer (3.5%), angular cheilitis (2.9%), multiple pigmented lesions (2.8%), hemangioma (2.3%), lichen planus (2.1%), leukoplakia (1.7%), recurrent aphthous stomatitis (1.4%), nicotine stomatitis (1.3%), median rhomboid glossitis (0.9%), actinic cheilitis (0.9%), pyogenic granuloma (0.7%), oral squamous papiloma (0.6%), and mucocele (0.2%). One case of oral cancer was observed. Different factors increased the probability of specific oral mucosal pathologies.Conclusions:  We can conclude that oral mucosal lesions are common in elderly people in Santiago, suggesting the necessity for improved standards of prevention, and diagnostic and opportune treatment of these lesions.
Orofacial granulomatosis: presentation, pathology and management of 13 cases
Authors: Sciubba James J. , Said‐Al‐Naief Nasser 
Published: October 2003

AbstractBackground: Orofacial granulomatosis (OFG) comprises of a group of conditions, all characterized histologically by the presence of granulomatous inflammation.Methods: This diagnosis may be determined by exclusion from other conditions that may present with similar clinical and histopathologic features. These include Melkersson–Rosenthal syndrome (MRS), Miescher's cheilitis (an oligosymptomatic form of MRS), Crohn's disease, and sarcoidosis.Results: A great deal of attention has been devoted to the similarity and overlap in clinicopathologic and histomorphologic features of these conditions, suggesting that they may actually represent a spectrum within a single overarching entity. In the review of the 13 cases of OFG retrieved from the files of the Long Island Jewish Medical Center, Department of Dental Medicine, we describe their presentation, clinicopathologic features, and management. These cases comprise examples of MRS (in its oligosymptomatic forms) and Crohn's disease. The similarity, kinship, and overlap between the cases presented are clearly demonstrated.Conclusion: In addition, based upon our observations and review, we propose the notion that oral manifestations of Crohn's disease may be classified as an oligosymptomatic form of MRS.
Pheochromocytoma associated with pregnancy
Authors: Kalra Jaswinder K. , Jain Vanita , Bagga Rashmi , Gopalan Sarala , Bhansali Anil K. , Behera Arunanshu , Batra Yatinder K. 
Published: October 2003

AbstractPheochromocytoma associated with pregnancy is rare with potentially lethal consequences. Antepartum diagnosis improves the maternal and perinatal outcome. The issue of mode of delivery is unresolved. Its definitive treatment is surgical resection preceded by medical management. Surgical resection may be done during caesarean section as is reported in the present case.
Ovarian endodermal sinus tumor in a 76‐year‐old woman
Authors: Filiz Gülaydan , Ozuysal Sema , Bilgin Tufan 
Published: October 2003

AbstractA 76‐year‐old woman underwent surgery for pelvic mass, during which a 13 × 8‐cm right ovarian tumor was discovered. On histopathological examination, she was diagnosed with an endodermal sinus tumor with right tubal metastasis. The patient was treated with four cycles of Bleomycin, Etoposide and Cisplatin. She died of disseminated disease four years later.
Cotyledonary‐like placenta associated with severe intrauterine growth restriction
Authors: Kita Naoko , Ohira Satoshi , Ashida Takashi , Kanai Makoto , Mitsuhashi Yuko , Konishi Ikuo 
Published: October 2003

AbstractThere have been no previous reports of human placentas mimicking the cotyledonary placenta of ruminants. We report a case of cotyledonary‐like placenta associated with severe intrauterine growth restriction.A woman pregnant for the first time was referred to our hospital at 27 weeks’ gestation because of severe intrauterine growth restriction. Characteristics of the placenta in the patient were examined by ultrasonographical and histopathological techniques. Ultrasonography revealed that a hypo‐echoic area intermingled with small segmented tissues in the placenta. The hypo‐echoic area changed in size and shape according to uterine contractions. At 31 weeks’ gestation, a caesarean section was performed because of non‐reassuring fetal status. A female baby weighing 814 g was delivered. The placenta, which weighed 260 g, contained several long stem villi running over a distance of 6 to 7 cm without branching into intermediate or terminal villi. Small villous tissues had developed only at the tips of these stem villi. The present case shows an extraordinarily abnormal development of the villous trees in the placenta, which may have caused fetal IUGR and non‐reassuring fetal status.
Complete hydatidiform mole in a triplet pregnancy coexisting two viable fetuses: Case report and review of the literature
Authors: Takagi Kimiyo , Unno Nobuya , Hyodo Hiro‐e M. , Hyodo Hironobu , Kashima Hiroyasu , Kubota Noriko , Ogiso Yoshihumi , Noike Masami , Itoh Kazuko , Shiobara Shigeki , Konishi Ikuo , Saito Shigeru 
Published: October 2003

AbstractWe report a rare case of a complete hydatidiform mole with two or more coexisting fetuses where both infants survived without complications. A male infant weighing 1258 g and a female infant weighing 880 g were delivered without complications and discharged 95 days after the birth. The analysis of DNA microsatellite polymorphisms indicated that the mole was of paternal origin and probably homozygous. The mother suffered from multiple pulmonary metastasis of the hydatidiform mole which was detected 3 days after the surgery and was successfully treated with methotrexate. A complete hydatidiform mole with two or more coexisting fetuses produces a dilemma between immediate termination and pregnancy continuation. Although the present case resulted in a favorable outcome, a review of the 14 reported cases suggests that the high fetal loss rate (90%) must be a consideration in the decision regarding management of such a pregnancy.
Cardiac failure caused by severe pre‐eclampsia with placental abruption, and its treatment with anti‐hypertensive drugs
Authors: Aoyama Kazufumi , Suzuki Yoshikatsu , Sato Takeshi , Yamamoto Tamao , Kojima Kazuhisa , Usami Takeshi , Ohte Nobuyuki , Suzumori Kaoru 
Published: October 2003

AbstractPre‐eclampsia is the abnormality of blood circulation in late pregnancy, often caused by renal failure, hemolysis, elevated liver enzyme, low platelet syndrome, and eclampsia. We present a case of severe pre‐eclampsia with placental abruption in a 24‐year‐old woman, pregnant for the first time. The patient was diagnosed with congestive heart failure, which came as a result of pre‐eclampsia. Anti‐hypertensive drugs were used for its treatment.
Preventable but not prevented: the reality of cervical cancer*
Authors: Saraiya Usha B. 
Published: October 2003

AbstractIntroduction: The incidence of invasive cervical cancer has decreased in the last 50 years in the developed countries substantially due to the use of routine pap smears. However, in the Asia–Oceanic region it continues to be high as screening programs are not established. Credit for starting cytology services in India goes to Professor P.N. Wahi of Agra. He became Founder President when about 34 cytologists got together in 1970 to form the Indian Academy of Cytologists. Since then cytology has spread through all parts of India. The Cytology Clinic in Cama & Albless Hospital was started in the same year. Since then over 100 000 women have been screened. Approximately 1200 cases of pre‐ and early cancers have been detected and treated. Since 1982 we are aware of the important role of human papillomavirus infection. We diagnose it by cytology and colposcopy and histology. Facilities for polymerase chain reaction, in‐situ hybridization and other virology studies are not available to us. CO2 laser treatment is found particularly useful in multicentric human papillomavirus disease.Screening for the State of Maharashtra: Since 1984 we have planned for a screening program for our State. We have a population of 78.9 million. Approximately 15 million women in the age group of 35–64 years have to be screened. The health care infrastructure is good with 36 medical colleges and over 35 district hospitals. Screening is planned in phases. Trained personnel are the key to a successful program. In the final analysis, cervical cancer is not just a biomedical disease. It has socio‐cultural and economic implications.
Lipid peroxidation and vitamin E status in gestational diabetes mellitus
Authors: Santra Debjyoti , Sawhney Harjit , Aggarwal Neelam , Majumdar Siddarth , Vasishta Kala 
Published: October 2003

AbstractAim: To investigate any correlation between plasma levels of lipid peroxides, antioxidant nutrient (α‐tocopherol) and oxidized high‐density lipoprotein (HDL) in patients with gestational diabetes and those with a normal pregnancy and the incidence of pre‐eclampsia.Methods: Sixty pregnant women attending an antenatal clinic were recruited for the study and were divided into two groups. Thirty women with gestational diabetes mellitus were recruited in the study group. The glucose‐tolerance‐test criteria, using 100 g of glucose taken orally, as laid down by the American College of Obstetricians and Gynecologists (1994) for diagnosis of gestational diabetes mellitus was used. Thirty gestation‐matched pregnant women with normal glucose tolerance test results were recruited as controls. A 10 mL venous blood sample was collected from each subject at the time of recruitment and thereafter at 4 week intervals until the time of delivery. Samples were analyzed for malondialdehyde thiobarbituric acid reactive, oxidized HDL‐cholesterol and α−tocopherol. The incidence of pre‐eclampsia and its correlation with antioxidant and lipid peroxide levels were compared in both the groups.Results: Ten subjects out of 30 in the study group and three subjects out of 30 in the control group developed pre‐eclampsia. The incidence of preterm labor in both the groups was same (16.66%). The mean lipid peroxide level was lower in the study group at recruitment and later the levels kept falling, whereas levels of α−tocopherol and oxidized‐HDL were higher in the study group and kept on rising at follow up.Conclusion: Gestational diabetes is not associated with increased levels of lipid peroxides and decreased levels of α‐tocopherol.
The outcomes of assisted reproductive technology cycles in patients with one or two ovaries
Authors: Levi Rafael , Özçakir Hasan Tayfun , Alatas Erkan , Göker Ege Nazan Tavmergen , Tavmergen Erol 
Published: October 2003

AbstractAim: To provide a comparison between the follicular response to ovulation induction, and the pregnancy rate of women with a single or two ovaries, undergoing assisted reproductive technology.Methods: To evaluate the outcome of assisted reproductive technology, 46 treatment cycles in women with one ovary were compared to 123 cycles in women with two ovaries.Results: The mean age of the patients in the two groups were not similar (34.4 ± 3.8 and 33.1 ± 4.0, respectively). The one ovary‐group had significantly higher mean baseline Follicle Stimulating Hormone levels and required more ampoules for induction. Although the induction period was longer in the one‐ovary group, the outcome of the assisted reproductive technology in both groups was similar. The maximum E2 levels on the day of Human Chorionic Gonadotropin administration were significantly lower in the one‐ovary group, although endometrial thickness appeared to be the same in both groups. The patients with two ovaries had a significantly higher mean number of mature or immature oöcytes aspirated, as well as embryos transfered. Although there was no statistical difference between the two groups following assisted reproductive technology, the pregnancy rate in the two‐ovary group was more than double that of the one‐ovary group.Conclusion: The potential for success after in vitro fertilization is not impaired in women with one ovary.
Fetal hepatic hemangioma representing non‐reassuring pattern in fetal heart rate monitoring
Authors: Morimura Yutaka , Fujimori Keiya , Ishida Tomohiko , Ito Akiko , Nomura Yasuhisa , Sato Akira 
Published: October 2003

AbstractTo the best of our knowledge, this is the first report of a non‐reassuring fetal pattern caused by a hepatic hemangioma that was found during fetal heart rate monitoring. A 37 weeks’ gestation, a 32‐year‐old‐woman was referred to our hospital for evaluation of a rapidly growing fetal abdominal tumor. Fetal heart rate monitoring revealed a non‐reassuring pattern, and a cesarean section was performed as a result. Examination of the 2820 g female infant suggested a hepatic hemangioma accompanying an intratumor hemorrhage and coagulopathy. The infant died 2 days after birth. The autopsy confirmed that the cause of death was a result of a hepatic hemangioma. Frequent monitoring of fetuses and ultrasound examinations are necessary for determining the timing of delivery and for a favorable prognosis.
In utero diagnosis of an aneurysm of the vein of Galen causing hydrocephalus and heart failure
Authors: Hirose Masaya , Yomo Hiroko , Akiyama Minoru , Kimura Toshio , Noda Yoichi 
Published: October 2003

AbstractA prenatally diagnosed aneurysm of the vein of Galen was presented in the fetus of a patient referred to our hospital at 31 weeks of gestation. Ultrasonography demonstrated polyhydramnios, cardiomegaly, dilatation of the right atrium and superior vena cava, tricuspid valve regurgitation, hydrocephalus, and a large hypoechoic mass with blood flow in the suboccipital region. Skin edema was shown thereafter. A 3262‐g male was delivered by cesarean at 35 weeks of gestation. Computed tomography imaging demonstrated a large mass in the suboccipital region, after which thrombocytopenia appeared and the neonate died at 18 days of age.
Favourable effect of regular intake of fermented milk containing Lactobacillus johnsonii on Helicobacter pylori associated gastritis
Authors: Pantoflickova D. , Corthésy‐Theulaz I. , Dorta G. , Stolte M. , Isler P. , Rochat F. , Enslen M. , Blum A. L. 
Published: October 2003

SummaryBackground : Lactobacillus johnsonii (Lj1) had an in vitro and in vivo inhibitory effect on Helicobacter pylori. Fermented milk containing Lj1 (LC1), coadministered with antibiotics had a favourable effect on H. pylori gastritis.Aim : Evaluate the effect of LC1 intake without antibiotics on H. pylori gastritis.Methods : Fifty H. pylori positive healthy volunteers were randomised in a double‐blind study to LC1 or placebo. Gastric biopsies from the antrum and corpus were obtained before, and after 3 and 16 weeks of treatment, for histology and quantitative cultures.Results : Severity and activity of antral gastritis was reduced after 16‐week LC1 intake (pretreatment and 16‐week inflammatory cell score: 6.0 ± 0.8 vs. 5.3 ± 0.1; P = 0.04). H. pylori density decreased in the antrum after LC1 intake (3‐week: 4.4 ± 0.6; 16‐week: 4.3 ± 0.5 log10 colony forming units (cfu) vs. pretreatment 4.5 ± 0.4 log10 cfu; P = 0.04, respectively). Mucus thickness increased after 16 weeks of LC1 consumption (change of mucus thickness with LC1 and placebo in the antrum: 0.6 ± 1.3 vs. ‐0.2 ± 1.0, P = 0.01; in the corpus: 0.3 ± 1.1 vs. ‐0.6 ± 1.5, P = 0.03).Conclusion : LC1 intake had a favourable, albeit weak, effect on H. pylori associated gastritis, particularly in the antrum. Regular ingestion of fermented milk containing L. johnsonii may reduce the risk of developing disorders associated with high degrees of gastric inflammation and mucus depletion.
The CD36 protein functions as an immunogenic domain of the RT8 alloantigen
Authors: Mlejnek P. , Křen V. , Liška F. , Zídek V. , Landa V. , Kurtz T. W. , Pravenec M. 
Published: October 2003

SummaryCompletely concordant distributions of Cd36 and Rt8 deletion/null and wild‐type alleles among inbred and congenic strains, together with Western blot analysis of RT8/CD36 proteins, indicated that the CD36 protein functions as an immunogenic domain of the RT8 alloantigen.
Rapid denaturing high‐performance liquid chromatography (DHPLC) for mutation scanning of the transforming growth factor β3 gene using a novel proof‐reading polymerase
Authors: Bayat A. , Walter J. M. , Lamb H. , Ferguson M. W. J. , Ollier W. E. R. 
Published: October 2003

SummaryWe have utilized a novel variation on the conventional denaturing high‐performance liquid chromatography (DHPLC) technology, which we term rapid DHPLC, combining changes in instrumentation, cartridge technology and analysis conditions to enable significant increases in throughput to be achieved. In addition, the use of a novel proof‐reading polymerase for sample amplification with a low misincorporation rate enables simplification of the DHPLC patterns and hence enhanced mutation detection recognition. This scheme for increasing DHPLC throughput has been tested by scanning the transforming growth factor (TGF) β3 gene for the presence of mutations for which there is limited published or on‐line data available regarding the presence of gene polymorphisms. TGFβ isoforms have multiple roles in cell division, growth, proliferation, transformation and differentiation. TGFβ3 is a TGFβ cytokine isoform, and has an important role in embryogenesis, cell differentiation and wound healing. The TGFβ3 gene consists of seven exons and six introns spanning 43 000 bp of the human genome on chromosome 14q23–24. The rapid DHPLC approach enabled scanning of all seven exons and part of the promoter region (1000 bp upstream from exon 1 in the 5′‐flanking regions) of the TGFβ3 gene in 95 Caucasian individuals in only 8 days, in comparison to the 17 days it would have previously taken. Mutations were clearly identified in the promoter region of the TGFβ3 gene but were absent from the exonic regions. Understanding the genetic variations affecting the TGFβ3 gene is important as this molecule has multiple regulatory functions on a variety of cell types.
Analysis of Fc gamma receptor II (CD32) polymorphism in populations of African and South Asian ancestry reveals east–west geographic gradients of allele frequencies
Authors: Carrington C. V. F. , Norman P. J. , Vaughan R. W. , Kondeatis E. , Ramdath D. D. , Hameed K. , Stephens H. A. F. 
Published: October 2003

SummaryAnalysis of FcγRIIA alleles in Pakistanis and in Trinidadians of South Asian, African and mixed ancestry revealed no significant differences between Trinidadian South Asians and Pakistanis. H131 homozygotes were more common among Trinidadian South Asians than among Africans and those of mixed ancestry. Comparison with other populations revealed east–west geographic gradients of allele frequencies.