bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contemporary Prophylactic Antibiotic Practices and Adjunct Therapies in Autologous Fat Grafting Procedures: A Survey of The Aesthetic Society Members
Authors: Araya Sthefano , Chang Alexander H , Moss Civanni , Gubara Sarah M , Gebreyesus Maria T , Jordan Kenneth , Ruth Karen J , Baltodano Pablo , Patel Sameer A 
Published: January 2024

Abstract

Background
Autologous fat grafting (AFG) is a widely used surgical technique that involves extracting a patient's own adipose tissue and transferring it to different areas of the body. This practice is still evolving. Guidelines for antibiotic prophylaxis and use of adjuncts in plastic surgery are currently limited, with a notable absence of standardized guidelines for AFG.

Objectives
In this survey, we assess contemporary antibiotic practices and adjuncts in AFG procedures.

Methods
A 52-question survey was emailed to 3106 active members of The Aesthetic Society. Two hundred and ninety-three responses were recorded, representing a 9% response rate.

Results
We analyzed 288 responses. The most common AFG procedures were facial (38%), gluteal (34%), and breast (27%) augmentation. Preoperative antibiotics were used by 84.0% overall, with rates of 74.3%, 88.0%, and 92.7% in face, breast, and gluteal AFG, respectively. Lipoaspirate–antibiotic mixing was reported by 19.8%, mainly during gluteal AFG (46.9%), and less so in face (2.8%) and breast (8%) AFG. Notably, 46.9% of surgeons administered prolonged prophylaxis for 72 h or more. Tranexamic acid was utilized by 39.9% of the surveyed surgeons. Platelet-rich plasma was used by 5.6%. Doppler ultrasound was incorporated by 16.7% in AFG, with 21.5% in gluteal AFG, 14% in the face, and 19% in breast procedures.

Conclusions
In this survey, we offer insights into antibiotic practices and adjunct therapies in AFG, especially intraoperative antibiotic mixing. Practices among members of The Aesthetic Society vary from guidelines. It is crucial to standardize practices and conduct further research to pave the way for evidence-based guidelines in AFG.

Level of Evidence: 5
The spectrum of thrombotic microangiopathy related to monoclonal gammopathy
Authors: van Doorn Daan P C , Abdul-Hamid Myrurgia A , Frenken Leon A M , van Paassen Pieter , Timmermans Sjoerd A M E G ,   for the Limburg Renal Registry
Published: January 2024

ABSTRACT

Background
Recent studies showed a high prevalence of monoclonal gammopathy (MG) in patients with thrombotic microangiopathy (TMA) aged over 50 years and suggested that complement dysregulation is pivotal for the disease to develop. Here, we studied this premise in seven patients with TMA and coexisting MG.

Methods
Patients with TMA on kidney biopsy and/or peripheral blood were recruited from the prospective COMPETE cohort (NCT04745195) and Limburg Renal Registry. Patients were screened for complement dysregulation, including genetics/factor H autoantibodies (FHAA) and functional ex vivo testing on microvascular endothelial cells.

Results
Seven (8%) out of 84 patients with TMA presented with a coexisting MG. MG clustered in patients aged over 50 years (n/N = 6/32, 19%). C4 and/or C3 levels were low in three patients, while four patients presented with normal complement levels. None of the patients carried rare variants in complement genes. Massive ex vivo C5b9 formation on the endothelium was noted in one patient; purified IgG from this patient caused massive ex vivo C5b9 formation via the alternative pathway of complement activation, pointing to complement dysregulation in the fluid phase. Kidney biopsies from other nephropathies linked to MG rarely exhibited concurrent TMA (n/N = 1/27, 4%).

Conclusions
MG clustered in patients with TMA aged over 50 years. TMA and coexisting MG represents a heterogeneous disease spectrum, including a small subset of patients who may present with complement dysregulation.
Mitochondrial fatty acid oxidation is the major source of cardiac adenosine triphosphate production in heart failure with preserved ejection fraction
Authors: Sun Qiuyu , Güven Berna , Wagg Cory S , Almeida de Oliveira Amanda , Silver Heidi , Zhang Liyan , Chen Brandon , Wei Kaleigh , Ketema Ezra B , Karwi Qutuba G , Persad Kaya L , Vu Jennie , Wang Faqi , Dyck Jason R B , Oudit Gavin Y , Lopaschuk Gary D 
Published: January 2024

Abstract

Aims
Heart failure with preserved ejection fraction (HFpEF) is a prevalent disease worldwide. While it is well established that alterations of cardiac energy metabolism contribute to cardiovascular pathology, the precise source of fuel used by the heart in HFpEF remains unclear. The objective of this study was to define the energy metabolic profile of the heart in HFpEF.

Methods and results
Eight-week-old C57BL/6 male mice were subjected to a ‘2-Hit’ HFpEF protocol [60% high-fat diet (HFD) + 0.5 g/L of Nω-nitro-L-arginine methyl ester]. Echocardiography and pressure–volume loop analysis were used for assessing cardiac function and cardiac haemodynamics, respectively. Isolated working hearts were perfused with radiolabelled energy substrates to directly measure rates of fatty acid oxidation, glucose oxidation, ketone oxidation, and glycolysis. HFpEF mice exhibited increased body weight, glucose intolerance, elevated blood pressure, diastolic dysfunction, and cardiac hypertrophy. In HFpEF hearts, insulin stimulation of glucose oxidation was significantly suppressed. This was paralleled by an increase in fatty acid oxidation rates, while cardiac ketone oxidation and glycolysis rates were comparable with healthy control hearts. The balance between glucose and fatty acid oxidation contributing to overall adenosine triphosphate (ATP) production was disrupted, where HFpEF hearts were more reliant on fatty acid as the major source of fuel for ATP production, compensating for the decrease of ATP originating from glucose oxidation. Additionally, phosphorylated pyruvate dehydrogenase levels decreased in both HFpEF mice and human patient’s heart samples.

Conclusion
In HFpEF, fatty acid oxidation dominates as the major source of cardiac ATP production at the expense of insulin-stimulated glucose oxidation.
Quantification of right ventricular amyloid burden with 18F-florbetapir positron emission tomography/computed tomography and its association with right ventricular dysfunction and outcomes in light-chain amyloidosis
Authors: Datar Yesh , Clerc Olivier F , Cuddy Sarah A M , Kim Sirwoo , Taylor Alexandra , Neri Jocelyn Canseco , Benz Dominik C , Bianchi Giada , Yee Andrew J , Sanchorawala Vaishali , Ruberg Frederick L , Landau Heather , Liao Ronglih , Kijewski Marie Foley , Jerosch-Herold Michael , Kwong Raymond Y , Di Carli Marcelo F , Falk Rodney H , Dorbala Sharmila 
Published: January 2024

Abstract

Aims
In systemic light-chain (AL) amyloidosis, quantification of right ventricular (RV) amyloid burden has been limited and the pathogenesis of RV dysfunction is poorly understood. Using 18F-florbetapir positron emission tomography/computed tomography (PET/CT), we aimed to quantify RV amyloid; correlate RV amyloid with RV structure and function; determine the independent contributions of RV, left ventricular (LV), and lung amyloid to RV function; and associate RV amyloid with major adverse cardiac events (MACE: death, heart failure hospitalization, cardiac transplantation).

Methods and results
We prospectively enrolled 106 participants with AL amyloidosis (median age 62 years, 55% males) who underwent 18F-florbetapir PET/CT, magnetic resonance imaging, and echocardiography. 18F-florbetapir PET/CT identified RV amyloid in 63% of those with and 40% of those without cardiac involvement by conventional criteria. RV amyloid burden correlated with RV ejection fraction (EF), RV free wall longitudinal strain (FWLS), RV wall thickness, RV mass index, N-terminal pro-brain natriuretic peptide, troponin T, LV amyloid, and lung amyloid (each P &lt; 0.001). In multivariable analysis, RV amyloid burden, but not LV or lung amyloid burden, predicted RV dysfunction (EF P = 0.014; FWLS P &lt; 0.001). During a median follow-up of 28 months, RV amyloid burden predicted MACE (P &lt; 0.001).

Conclusion
This study shows for the first time that 18F-florbetapir PET/CT identifies early RV amyloid in systemic AL amyloidosis prior to alterations in RV structure and function. Increasing RV amyloid on 18F-florbetapir PET/CT is associated with worse RV structure and function, predicts RV dysfunction, and predicts MACE. These results imply a central role for RV amyloid in the pathogenesis of RV dysfunction.
Cardiac radiotherapy transiently alters left ventricular electrical properties and induces cardiomyocyte-specific ventricular substrate changes in heart failure
Authors: Mehrhof Felix , Hüttemeister Judith , Tanacli Radu , Bock Matthias , Bögner Markus , Schoenrath Felix , Falk Volkmar , Zips Daniel , Hindricks Gerhard , Gerds-Li Jin-Hong , Hohendanner Felix 
Published: January 2024

Abstract

Aims
Ongoing clinical trials investigate the therapeutic value of stereotactic cardiac radioablation (cRA) in heart failure patients with ventricular tachycardia. Animal data indicate an effect on local cardiac conduction properties. However, the exact mechanism of cRA in patients remains elusive. Aim of the current study was to investigate in vivo and in vitro myocardial properties in heart failure and ventricular tachycardia upon cRA.

Methods and results
High-density 3D electroanatomic mapping in sinus rhythm was performed in a patient with a left ventricular assist device and repeated ventricular tachycardia episodes upon several catheter-based endocardial radio-frequency ablation attempts. Subsequent to electroanatomic mapping and cRA of the left ventricular septum, two additional high-density electroanatomic maps were obtained at 2- and 4-month post-cRA. Myocardial tissue samples were collected from the left ventricular septum during 4-month post-cRA from the irradiated and borderzone regions. In addition, we performed molecular biology and mitochondrial density measurements of tissue and isolated cardiomyocytes. Local voltage was altered in the irradiated region of the left ventricular septum during follow-up. No change of local voltage was observed in the control (i.e. borderzone) region upon irradiation. Interestingly, local activation time was significantly shortened upon irradiation (2-month post-cRA), a process that was reversible (4-month post-cRA). Molecular biology unveiled an increased expression of voltage-dependent sodium channels in the irradiated region as compared with the borderzone, while Connexin43 and transforming growth factor beta were unchanged (4-month post-cRA). Moreover, mitochondrial density was decreased in the irradiated region as compared with the borderzone.

Conclusion
Our study supports the notion of transiently altered cardiac conduction potentially related to structural and functional cellular changes as an underlying mechanism of cRA in patients with ventricular tachycardia.
Seafloor incubation experiments at deep-sea hydrothermal vents reveal distinct biogeographic signatures of autotrophic communities
Authors: Fullerton Heather , Smith Lindsey , Enriquez Alejandra , Butterfield David , Wheat C Geoffrey , Moyer Craig L 
Published: January 2024

Abstract
The discharge of hydrothermal vents on the seafloor provides energy sources for dynamic and productive ecosystems, which are supported by chemosynthetic microbial populations. These populations use the energy gained by oxidizing the reduced chemicals contained within the vent fluids to fix carbon and support multiple trophic levels. Hydrothermal discharge is ephemeral and chemical composition of such fluids varies over space and time, which can result in geographically distinct microbial communities. To investigate the foundational members of the community, microbial growth chambers were placed within the hydrothermal discharge at Axial Seamount (Juan de Fuca Ridge), Magic Mountain Seamount (Explorer Ridge), and Kamaʻehuakanaloa Seamount (Hawai'i hotspot). Campylobacteria were identified within the nascent communities, but different amplicon sequence variants were present at Axial and Kamaʻehuakanaloa Seamounts, indicating that geography in addition to the composition of the vent effluent influences microbial community development. Across these vent locations, dissolved iron concentration was the strongest driver of community structure. These results provide insights into nascent microbial community structure and shed light on the development of diverse lithotrophic communities at hydrothermal vents.
Light irradiation changes the regulation pattern of BtCrgA on carotenogenesis in Blakeslea trispora
Authors: Yang Jiamin , Zeng Mingxi , Wu Hui , Han Zhenlin , Du Zhiyan (Rock) , Yu Xiaobin , Luo Wei 
Published: January 2024

Abstract
CrgA has been shown to be a negative regulator of carotenogenesis in some filamentous fungi, while light irradiation is an inducible environmental factor for carotenoid biosynthesis. To clarify the relationship between CrgA and light-inducible carotenogenesis in Blakeslea trispora, the cis-acting elements of the btcrgA promoter region were investigated, followed by the analyses of correlation between the expression of btcrgA and carotenoid structural genes under different irradiation conditions. A variety of cis-acting elements associated with light response was observed in the promoter region of btcrgA, and transcription of btcrgA and carotenoid structural genes under different irradiation conditions was induced by white light with a clear correlation. Then, RNA interference and overexpression of btcrgA were performed to investigate their effects on carotenogenesis at different levels under irradiation and darkness. The analyses of transcription and enzyme activities of carotenoid structural gene, and accumulation of carotenoids among btcrgA-interfered, btcrgA-overexpressed, and wild-type strains under irradiation and darkness indicate that btcrgA negatively regulates the synthesis of carotenoid in darkness, while promotes the carotenogenesis under irradiation regardless of reduced or overexpression of btcrgA .
Are the magnitudes of earthquakes in Southern California, with incompleteness removed, correlated?
Authors: Taroni Matteo 
Published: January 2024

SUMMARY
No.
The correlation between the magnitudes of earthquakes is a scientific question usually investigated by statistical seismologists. In the last decades, two opposite answers have been given to the problem of correlation of magnitudes: there is no correlation, that is the magnitude of earthquakes in a seismic catalogue can be considered as random sampling from the magnitude distribution; or, there is a correlation, that is the magnitude of a seismic event can influence the magnitude of the successive event. Here we used the Southern California seismic catalogue, properly treated to remove incompleteness, to answer the question, finding no significant correlation between magnitudes.
An improved estimation of stress drop and its application on induced earthquakes in the Weiyuan Shale Gas Field in China
Authors: Zhang Jiewen , Yang Hongfeng , Zi Jinping , Su Jinrong , Chen Xiaowei 
Published: January 2024

SUMMARY
Stress drop is a proxy of understanding earthquake source process, and it is controversial whether the stress drops of induced earthquakes associated with hydraulic fracturing and injection activities are similar to those of tectonic earthquakes. The measurement of stress drops is usually biased due to the limitations of observation means, or hidden issues in the estimation approaches. Utilizing a local short-period seismic network, we investigate the stress drops of induced earthquakes in Weiyuan Shale Gas Field in Sichuan Province, China from 2019 to 2020. Totally 11 844 earthquakes are involved in the analysis, and their stress drops are obtained using an improved approach on the basis of the traditional spectral decomposition method combined with a global optimization algorithm to avoid stacking of spectra that is found leading to source parameter underestimation. We divide the studied area into three subareas, and the results show strong stress drop heterogeneity across the entire region. We obtain an average stress drop of 2.29 MPa, piecewise stress drop dependence to earthquake magnitude, and complex depth dependence pattern. Our results indicate that stress drops of induced earthquakes are overall consistent with the induced earthquakes in other areas as well as tectonic earthquakes in different environments. Meanwhile, the complexity in the stress drop dependence to depth possibly reflects the variability of stress drops for different earthquake triggering mechanisms.
Cuticular wax biosynthesis in blueberries (Vaccinium corymbosum L.): Transcript and metabolite changes during ripening and storage affect key fruit quality traits
Authors: Yan Yifan , Gagalova Kristina K , Gerbrandt Eric M , Castellarin Simone D 
Published: January 2024

Abstract
In fruits, cuticular waxes affect fruit quality traits such as surface color at harvest and water loss during postharvest storage. This study investigated the transcriptional regulation of cuticular wax deposition in northern highbush blueberries (Vaccinium corymbosum L.) in relation to fruit water loss and surface color during ripening and postharvest storage, as well as the effects of abscisic acid (ABA)-mediated changes in cuticular wax deposition on these fruit quality traits. Total cuticular wax content (μg∙cm−2) decreased during fruit ripening and increased during postharvest storage. Transcriptome analysis revealed a transcript network for cuticular wax deposition in blueberries. Particularly, five OSC-Likes were identified as putative genes for triterpene alcohol production, with OSC-Like1 and OSC-Like2 encoding mixed amyrin synthases, OSC-Like3 encoding a lupeol synthase, and OSC-Like4 and OSC-Like5 encoding cycloartenol synthases. The expression of three CYP716A-like genes correlated to the accumulation of two triterpene acids oleanolic acid and ursolic acid, the major wax compounds in blueberries. Exogenous ABA application induced the expression of triterpenoid biosynthetic genes and the accumulation of β-amyrin and oleanolic acid, as well as increased the ratio of oleanolic acid to ursolic acid. These changes were associated with reduced fruit water loss. The content of β-diketones was also increased by ABA application, and this increase was associated with increased fruit lightness (measured as L* using CIELAB Color Space by a colorimeter). This study provided key insights on the molecular basis of cuticular wax deposition and its implications on fruit quality traits in blueberries.
NATO in Kosovo and the logic of successful security practices
Authors: Newman Edward , Visoka Gëzim 
Published: January 2024

Abstract
NATO's involvement in Kosovo and its transformation from a military intervention to a UN-authorized peacekeeping operation has played a key role in the evolution of the alliance's strategic and political agenda. This article explores the importance of NATO's engagement with Kosovo in retrospect and in relation to the future outlook for European and global peace and security. It focuses on the centrality of this case for NATO's post-Cold War identity and as a key issue in the renewed political conflict associated with the transitional international order. We make three arguments. First, we argue that NATO's involvement in Kosovo has shaped and exacerbated the broader friction associated with the shifting international order. Second, we make the case that this engagement has played a key role in the strategic, political and operational development of NATO and its evolving identity. Thirdly, as a result of this, we argue that NATO's evolution and transformation in Kosovo was guided by what we call the logic of successful security practices. This entails making prudent political choices and implementing practical steps to harmonize divergent strategic interests among NATO members, adapting to shifting security circumstances, and upholding the organization's significance, credibility and influence both within and beyond the Euro-Atlantic community.
Elderly patients benefit from minimally invasive mitral valve surgery: perioperative risk management matters
Authors: Cocchieri Riccardo , Mousavi Iman , Verbeek Eva C , Riezebos Robert K , Yazdanbakhsh Aria P , de Mol Bas A M J 
Published: January 2024

Abstract

OBJECTIVES
The goal was to assess the single-centre results of minimally invasive mitral valve surgery (MIMVS) in the elderly population.

METHODS
All patients referred for minimally invasive valve surgery underwent a standardized preoperative screening. We performed a retrospective analysis of 131 consecutive elderly patients (≥75 years) who underwent endoscopic MIMVS through a right mini-thoracotomy. Survival and postoperative course were assessed in 2 groups: a repair group and a replacement group.

RESULTS
Eighty-five patients underwent mitral valve repair, and 46 had mitral valve replacement. The mean age was 79 ± 2.9 years, and the median follow-up duration was 3.8 years. The cardiopulmonary bypass time (128.7 min vs 155.9 min, P = 0.012) and the cross-clamp time (84.9 min vs 124.1 min, P = 0.005) were significantly longer in the replacement group. Except for more reinterventions for bleeding in the replacement group (10.9% vs 0%, P = 0.005), there were no significant differences in the postoperative course between the 2 groups. Low mortality rates at the midterm follow-up were observed in both groups, and no differences were observed between the 4-and the 12-month follow-up. Survival rates after 1 year and 5 years were 97.6% and 88.6%, respectively, with no significant differences between the 2 groups.

CONCLUSIONS
MIMVS is an excellent treatment option in vulnerable elderly patients with excellent short- and long-term results. Although other studies suggest that repair could be superior to replacement even in older patients, our experience suggests that replacement is equivalent to repair in terms of mortality and major adverse cardiac and cerebrovascular events. Experience and standardized preoperative screening are mandatory to achieve optimal results.
The Movable Type Used by Paul Perny in His Dictionnaire français-latin-chinois de la langue mandarine parlée and His Contribution to the Typography of the Chinese Script in France
Authors: Lee-Lee Xavier , Trujillo-González Verónica C 
Published: January 2024

Abstract
The printing of Chinese characters, as well as their combination with alphabetic letters, has been one of the great challenges for Western typographers. When the missionary Paul Perny published his Dictionnaire français-latin-chinois de la langue mandarine parlée [French-Latin-Chinese dictionary of the spoken Mandarin language] (1869) in Paris, the conceptual design of the structure of its entries as well as the typographical quality of the Chinese characters he used were outstanding in their functionality and, hence, at a level far more advanced than the dictionaries of Chinese in combination with Western languages published in France up to that time. This paper explores the factors that enabled this dictionary to mark a turning point in the printing of Chinese characters in France.