bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host individual and gut location are more important in gut microbiota community composition than temporal variation in the marine herbivorous fish Kyphosus sydneyanus
Authors: Pisaniello Alessandro , Handley Kim M. , White W. Lindsey , Angert Esther R. , Boey Jian Sheng , Clements Kendall D. 
Published: September 2023

Abstract
Background
Gut microbiota play a key role in the nutrition of many marine herbivorous fishes through hindgut fermentation of seaweed. Gut microbiota composition in the herbivorous fish Kyphosus sydneyanus (family Kyphosidae) varies between individuals and gut sections, raising two questions: (i) is community composition stable over time, especially given seasonal shifts in storage metabolites of dietary brown algae, and (ii) what processes influence community assembly in the hindgut?

Results
We examined variation in community composition in gut lumen and mucosa samples from three hindgut sections of K. sydneyanus collected at various time points in 2020 and 2021 from reefs near Great Barrier Island, New Zealand. 16S rRNA gene analysis was used to characterize microbial community composition, diversity and estimated density. Differences in community composition between gut sections remained relatively stable over time, with little evidence of temporal variation. Clostridia dominated the proximal hindgut sections and Bacteroidia the most distal section. Differences were detected in microbial composition between lumen and mucosa, especially at genus level.

Conclusions
High variation in community composition and estimated bacterial density among individual fish combined with low variation in community composition temporally suggests that initial community assembly involved environmental selection and random sampling/neutral effects. Community stability following colonisation could also be influenced by historical contingency, where early colonizing members of the community may have a selective advantage. The impact of temporal changes in the algae may be limited by the dynamics of substrate depletion along the gut following feeding, i.e. the depletion of storage metabolites in the proximal hindgut. Estimated bacterial density, showed that Bacteroidota has the highest density (copies/mL) in distal-most lumen section V, where SCFA concentrations are highest. Bacteroidota genera Alistipes and Rikenella may play important roles in the breakdown of seaweed into useful compounds for the fish host.
Economic evaluation of a multimorbidity patient centered care model implemented in the Chilean public health system
Authors: Zamorano Paula , Espinoza Manuel Antonio , Varela Teresita , Abbott Tomas , Tellez Alvaro , Armijo Nicolás , Suarez Francisco 
Published: September 2023

AbstractMultimorbidity and patient-centered care approaches are growing challenges for health systems and patients. The cost of multimorbidity patients and the transition to a new care strategy is still sightly explored. In Chile, more than 70% of the adult population suffer from multimorbidity, opening an opportunity to implement a Multimorbidity patient-centered care model. The objective of this study was to perform an economic evaluation of the model from the public health system perspective.The methodology used a cost-consequence evaluation comparing seven exposed with seven unexposed primary care centers, and their reference hospitals. It followed three steps. First, we performed a Time-Driven Activity-Based Costing with routinely collected data routinely collected. Second, we run a comparative analysis through a propensity score matching and an estimation of the attributable costs to health services utilization at primary, secondary and tertiary care and health outcomes. Third, we estimated implementation and transaction costs.Results showed savings in aggregate costs of the total population (-0.12 (0.03) p < 0.01) during the period under evaluation. Costs in primary care showed a significant increase, whereas tertiary care showed significant savings. Health outcomes were associated with higher survival in patients under the new care model (HR 0.70 (0.05) p < 0.01). Implementation and transaction costs increased as the number of pilot intervention centers increased, and they represented 0,07% of the total annual budget of the Servicio de Salud Metropolitano Sur Oriente. After three years of piloting, the implementation and transaction cost for the total period was USD 1,838,767 and 393,775, respectively.The study’s findings confirm the purpose of the new model to place primary health care at the center of care for people with non-communicable chronic diseases. Thus, it is necessary to consider implementation and transaction costs to introduce a broad health system multimorbidity approach. The health system should assume some of them permanently to guarantee sustainability and facilitate scale-up.
Neck stiffness and bone osteolytic lesion in a 3-years old child: a case report
Authors: Carraro Carolina , Rabusin Marco , Murru Flora Maria , Ammar Lydie , Barbi Egidio , Amaddeo Alessandro , Cozzi Giorgio 
Published: September 2023

Abstract
Background
Neuroblastoma is the most frequent extracranial solid tumor occurring in childhood, representing approximately 28% of all cancers diagnosed in infants. Signs and symptoms of neuroblastoma vary with the site of development of the tumor and can mimic other diseases due to its extreme clinical variability. However, torticollis is not reported in the medical literature as a leading symptom of neuroblastoma.

Case presentation
Here we report the case of a 3 years-old girl with fever and neck stiffness. Blood tests revealed a mild anemia and a rise in inflammatory markers. CT-scan showed a solid, heterogeneous, predominantly hypodense surrenal mass with eccentric calcification and extensive inhomogeneity of the vertebral metamers. Blood tests revealed raised serum levels of Neuron-Specific Enolase. At the 24-hours urine collection urinary catecholamines were greatly increased. A course of chemotherapy for neuroblastoma was promptly started with immediate clinical improvement.

Conclusions
This case shows that the presence of torticollis could be a chief complaint of neuroblastoma. To our knowledge, neuroblastoma is not mentioned among life-threatening underlying conditions of torticollis in most recent literature reviews.
Periampullary tumors in a patient with pancreatic divisum and neurofibromatosis type 1: a case report
Authors: Li Bin-bin , Zheng Hui , Lou Yi-Dan , Zhang Wen-Wei , Zheng Song 
Published: September 2023

Abstract
Introduction
We present a case of a male patient with neurofibromatosis type 1 diagnosed with pancreatic divisum and several gastrointestinal tumors. A 55-year-old man was admitted to the hospital with recurrent chronic pancreatitis, indicating a large mass in the ampulla. In addition, genetic testing revealed two unique germline mutations in the neurofibromin (NF1) gene, and their potential interaction in promoting cancer was further investigated.

Conclusion
The first similar case was reported in 2020. The current case was distinct from other cases since an additional two NF1 mutations were found in the patient. In conjunction with prior case reports, our findings imply that genetic testing in patients diagnosed with neurofibromatosis type 1 could be helpful in the development of effective treatments.
Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR
Authors: Skoufou-Papoutsaki Nefeli , Adler Sam , D'Santos Paula , Mannion Liz , Mehmed Shenay , Kemp Richard , Smith Amy , Perrone Francesca , Nayak Komal , Russell Alasdair , Zilbauer Matthias , Winton Douglas J. 
Published: September 2023

ABSTRACT
Organoids, combined with genetic editing strategies, have the potential to offer rapid and efficient investigation of gene function in many models of human disease. However, to date, the editing efficiency of organoids with the use of non-viral electroporation methods has only been up to 30%, with implications for the subsequent need for selection, including turnaround time and exhaustion or adaptation of the organoid population. Here, we describe an efficient method for intestinal organoid editing using a ribonucleoprotein-based CRISPR approach. Editing efficiencies of up to 98% in target genes were robustly achieved across different gut anatomical locations and developmental timepoints from multiple patient samples with no observed off-target editing. The method allowed us to study the effect of loss of the tumour suppressor gene PTEN in normal human intestinal cells. Analysis of PTEN-deficient organoids defined phenotypes that likely relate to its tumour suppressive function in vivo, such as a proliferative advantage and increased organoid budding. Transcriptional profiling revealed differential expression of genes in pathways commonly known to be associated with PTEN loss, including mTORC1 activation.
Monitoring of magnetospheric parameters based on cosmic ray effects in August 2018
Authors: Kovalev Ivan , Olemskoy Sergey , Sdobnov Valeriy 
Published: September 2023

Using data (uncorrected for the temperature effect) from the global network of neutron monitors (GNNM), along with data from the Yakutsk muon telescope complex and the muon hodoscope URAGAN (Moscow), we have applied a modified spectrographic global survey (SGS) method to the 2018 August event in order to split cosmic ray variations into components of primary, magnetospheric, and atmospheric origin. We obtained time variations in the 4 GV-rigidity primary particle isotropic flux and pitch-angle anisotropy, as well as in the interplanetary magnetic field (IMF) orientation. We also showed variations in the geomagnetic cutoff rigidity (GCR) in Irkutsk.
Using the obtained data on the changes in the planetary system of GCR within a simple model of a bounded magnetosphere, we have calculated some parameters of magnetospheric current systems, namely, the ring current radius, the magnetopause current radius, and the Dst index.
Digital workflows part 2: applications of digital technology in orthognathic surgical planning – a case series
Authors: Keating Chris , Haworth Jennifer , Ireland Tony , Bousfield Joanne , Felstead Andrew , Parvizi Farnaz 
Published: September 2023

This is the second article in a two-part series considering the relevance and clinical uses of digital technologies in relation to orthodontics. The aim is to take a closer look at the application of digital technology in relation to joint orthodontic/orthognathic treatment and present two clinical cases that have undergone treatment by means of a digital workflow. CPD/Clinical relevance: Digital technologies can enhance pre-operative orthognathic planning
Treatment of rheumatoid arthritis with baricitinib or upadacitinib is associated with reduced scaffold protein NEDD9 levels in CD4+ T cells
Authors: Golumba‐Nagy Viktoria , Yan Shuaifeng , Steinbach‐Knödgen Eva , Thiele Jan , Esser Ruth L. , Haak Thomas H. , Nikiforov Anastasia , Meyer Anja , Seeger‐Nukpezah Tamina , Kofler David M. 
Published: September 2023

AbstractThe JAK/STAT pathway plays a crucial role in the pathogenesis of rheumatoid arthritis (RA) and JAK inhibitors have emerged as a new group of effective drugs for RA treatment. Recently, high STAT3 levels have been associated with the upregulation of the scaffold protein NEDD9, which is a regulator of T‐cell trafficking and promotes collagen‐induced arthritis (CIA). In this study, we aimed to reveal how treatment with JAK inhibitors affects NEDD9 in CD4+ T cells from RA patients. We analyzed NEDD9 expression in CD4+ T cells from 50 patients treated with either baricitinib, tofacitinib, or upadacitinib and performed cell migration assays to assess the potential influence of JAK inhibitor treatment on CD4+ T‐cell migration. We observed that treatment with baricitinib and upadacitinib is associated with reduced NEDD9 expression in CD4+ T cells. In contrast, NEDD9 levels were not altered during treatment with tofacitinib. Moreover, treatment with baricitinib was associated with a significantly reduced migratory capacity of effector CD4+ T cells but not with impaired migration of Treg cells. This study reveals previously unknown associations between JAK inhibitor treatment and NEDD9 expression and indicates that JAK inhibitors could reduce effector T‐cell migration.
Experimental and theoretical investigation of the structural behavior of reinforced glulam wooden members by NSM steel bars and shear reinforcement CFRP sheet
Authors: Alkhudery Hayder H. , Al-Tameemi Haider Ali , Al-Katib Haider A. A. 
Published: September 2023

Abstract
Reinforcing the wooden structural members is considered as a challenging matter to overcome the drawbacks of using wood in the construction field. The current investigation, therefore, presents an attempt to evaluate the effectiveness of utilizing steel bars as reinforcements in glulam timber beams using the near-surface-mounted (NSM) technique in conjunction with carbon fiber-reinforced polymer (CFRP) sheet wraps. A series of flexural testing was carried out until failure for both reinforced and unreinforced glulam members in a simple support system. Eleven specimens were examined in two groups to compare them with the control beam. Five reinforced glulam (RG) beams of the first group were reinforced with different schemes at tension and compression zones using the same bar size. The other five specimens of the second group were reinforced with shear reinforcement using fully wrapped strips made of CFRP sheet in addition to the same flexural reinforcement schemes as the first group. Each glulam beam has a span of 1.35 m and a rectangular section sized 85 mm × 175 mm. Based on experimental outcomes, theoretic modeling was provided to estimate the ultimate load capacity and bending rigidity of reinforcing glulam members. Though several theoretical predictions of flexural capacity were overstated when compared to experimental predictions, these disparities were frequently about 10%, confirming that the proposed theoretical model was accurate, and the mean of ultimate loading capacity and deflection between experimental and theoretical results were 1.01 and 1.09, respectively. Experimental results presented show that the RG beams performed much better than the unreinforced reference beams in terms of structural behavior, with improvements in ultimate load capacity ranging from 16 to 49%. On the other hand, the shear reinforcement for RG members slightly improved flexural performance, and the ultimate load capacity increased by 2–7%. Therefore, it was concluded that the NSM techniques using ordinary steel bars were effective ways in strengthening the glulam members in terms of flexural stiffness with increasing ultimate load capacity.
From Pariah to Baboon: Ludwig Robert’s Parody of Michael Beer’s Paria
Authors: Engelsberg Ezra Loren 
Published: September 2023

Abstract
Michael Beer’s successful 1823 play Der Paria is famous as an early complaint against the status of Jews as second-rate citizens. As such, it is fundamental to the conception of Jews as pariahs, best known through the writings of Hannah Arendt. Its long reception history has overlooked an important source, that is now uncovered for the first time in a hundred years. Beer’s play was parodied by Ludwig Robert with Der Pavian. Both authors were scions of Berlin’s Jewish elite, and among the first authors of Jewish descent to write in German. Robert’s parody shows why he did not share in the general enthusiasm about Der Paria, how the choice of the parodic genre was a self-referential critique, and how form and content of the parody together intervened in Beer’s use of the genre of Trauerspiel. Thus, it contributes to our understanding of the diverse strategies and self-perceptions of upper-class Berlin Jews, confronted with the limits of their pioneering the Jewish integration into non-Jewish society, showing the role of theater as a means of negotiating contemporary social problems both in private and publicly, stressing the difficult presence of Jews in the German public sphere.
Communicatio Idiomatum and the Lutheran Quest for Christological Agency
Authors: Małysz Piotr J. 
Published: September 2023

Abstract
This article compares Luther’s understanding of the unity of Christ’s person with that of his theological successors, focusing on Martin Chemnitz and Johann Gerhard. It argues that Luther’s more apophatic approach to the communicatio idiomatum and his heirs’ elaborate doctrine of the three genera of the communication do not stand in a straightforward relationship of continuity. To be sure, both are historically found in the context of Eucharistic polemics. Yet Luther’s perspective on the communicatio is driven by a desire to give expression to Christ-for-me specifically as the subject and agent of his promise; it has its driving force in the ontology of the promise as the essence of the gospel. By contrast, Luther’s successors are preoccupied with the ability of Christ’s assumed human nature to receive divine properties in the hope of shoring up the presence of Christ’s body in the Lord’s Supper. In the end, it is Luther who succeeds in presenting a more successful account of a co-extensive and internally congruent understanding of Christ’s divine and human agency, whereas his successors’ account of the unity of Christ remains undermined by intractable and ever-threatening divergence.
Disyllabic hypocoristics in Chilean Spanish: a stratal OT analysis
Authors: King Matthew 
Published: September 2023

Abstract
Hypocoristics have received considerable interest from phonologists in recent decades, particularly within the Optimality-theoretic literature. While most of these analyses have been situated in parallelist OT, I claim that this architectural choice entails hidden complexities. It is cross-linguistically common for multiple hypocoristics to be formed from a single proper noun, e.g., Matthew → Matt/Matty. In this case, parallelist OT must resort to co-phonologies to avoid ranking paradoxes, which results in significant analytical complexity. By contrast, I argue in this paper that a stratal analysis is crucial for capturing multiple patterns of Hypocoristic Formation (HF) within a single architecture. I present here data of disyllabic hypocoristics from Standard Chilean Spanish. These hypocoristics are derived from the proper nouns by means of two separate anchoring sites: to the left edge of the full name, or to prominence in the full name, i.e., the tonic syllable. In addition, hypocoristics may be prosodically- or morphologically-driven: they may solely be formed by truncating the material of the proper noun, or, in the latter case, there may also be additional suffixal material provided. These data, therefore, lend themselves to a four-way categorisation. I show that this categorisation can be fully accounted for within a stratal architecture, and without the need for additional co-phonologies. Within the analysis, I locate each of the anchoring sites within the lexical phonology: edge-anchoring occurs at the stem level, along with syllabification and stress assignment, while prominence-anchoring occurs at the word level. In this way, the input to edge-anchoring at the stem level comprises a string of segments, while the input to prominence-anchoring at the word-level contains prosodic units up to the foot, which permits word-level HF to anchor to prominence. I further posit that prosodic- and morphologically-driven HF are caused through the passing of hypocoristic morphemes from the morpho-syntax to the phonology. Purely prosodic HF is triggered through a null or covert morpheme, while morphological HF triggered by an overt one. The hypocoristic morphemes themselves are stored as diacritics in the proper nouns’ lexical entries. Furthermore, the constraints that select the hypocoristic forms as optimal are sensitive to the presence of this hypocoristic morpheme, which is reflected in tableaux through indexing. When a hypocoristic morpheme is present in the input of a proper noun to a particular stratum, these highly-ranked indexed constraints select a corresponding hypocoristic form as optimal. If the hypocoristic morpheme is absent, these constraints do not assign violations, and the proper noun is instead (vacuously) found optimal. This analysis thus unifies two distinct patterns of HF in one phonological grammar through the inclusion of indexed-constraints and the serial derivation in a constraint-based architecture.
The effect of polycyclic aromatic hydrocarbon biomarkers on cardiovascular diseases
Authors: Qigang Nie , Afra Arghavan , Ramírez-Coronel Andrés Alexis , Turki Jalil Abduladheem , Mohammadi Mohammad Javad , Gatea M. Abdulfadhil , Efriza  , Asban Parisa , mousavi Seyede Kosar , Kanani Parnia , Mombeni Kazemi Fatemeh , Hormati Maryam , Kiani Fatemeh 
Published: September 2023

Abstract
Polycyclic aromatic hydrocarbon (PAHs) are part of particulate matter (PM), which is produced from incomplete combustion of organic matter. Biomarkers mean biological indicators, molecules that indicate a normal or abnormal process in the body and may be a sign of a condition or disease. Studies show that PAHs increase the risk of cardiovascular diseases through processes such as oxidative stress, inflammation and atherosclerosis. The present study focused on the evaluation of health effects PAHs biomarkers on cardiovascular diseases (CVD). In this narrative study, data were collected from databases such as Scopus, PubMed, Web of science and Google Scholar in the period 1975–2023. After screening, duplicate and irrelevant articles were removed. Finally, 68 articles related to the effect of PAHs on CVD were included in the study. In addition to the articles found through the search in databases, another 18 articles from the references of the selected articles were included. According to the finding in during the biotransformation of PAH, a number of metabolites are made, such as phenols, diols, quinones, and epoxides. Phenolic isomers have the highest percentage and biomarkers used for their detection include 2-OHNAP used to trace naphthalene from heating processed food, 3-OHPHEN used to trace phenanthrene from diesel, 2-OHFLU used to trace fluorene and 1-OHPYR used to trace pyrene from cigarette and hookah smoke. According to the result, increasing blood pressure and heart rate and causing atherosclerosis are the main complications due to exposure to PAH metabolite on cardiovascular system. The most important agents that causes this affects including increased homocysteine, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), serum biomarkers of C-reactive protein, and triglycerides. Result this study showed that cardiovascular diseases risk is increased by exposure to PAH biomarkers from smoking, car emissions, occupational exposure, and incinerators. Therefore, strict controls should be implemented for sources of PAH production and exposure.