bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mettl3-Mediated m6A Methylation Controls Pancreatic Bipotent Progenitor Fate and Islet Formation
Authors: Sun Jiajun , Wang Yanqiu , Fu Hui , Kang Fuyun , Song Jiaxi , Xu Min , Ning Guang , Wang Jian , Wang Weiqing , Wang Qidi 
Published: November 2023

The important role of m6A RNA modification in β-cell function has been established; however, how it regulates pancreatic development and endocrine differentiation remains unknown. Here, we generated transgenic mice lacking RNA methyltransferase-like 3 (Mettl3) specifically in Pdx1+ pancreatic progenitor cells and found the mice with the mutation developed hyperglycemia and hypoinsulinemia at age 2 weeks, along with an atrophic pancreas, reduced islet mass, and abnormal increase in ductal formation. At embryonic day 15.5, Mettl3 deletion had caused a significant loss of Ngn3+ endocrine progenitor cells, which was accompanied by increased Sox9+ ductal precursor cells. We identified histone deacetylase 1 (Hdac1) as the critical direct m6A target in bipotent progenitors, the degeneration of which caused abnormal activation of the Wnt/Notch signaling pathway and blocked endocrine differentiation. This transformation could be manipulated in embryonic pancreatic culture in vitro through regulation of the Mettl3-Hdac1-Wnt/Notch signaling axis. Our finding that Mettl3 determines endocrine lineage by modulating Hdac1 activity during the transition of bipotent progenitors might help in the development of targeted endocrine cell protocols for diabetes treatment.

Article Highlights
Testing of Regulating and Non-Regulating Hydraulic Pumps
Authors: Kosiba Ján , Čurgaliová Gabriela , Tkáč Zdenko , Jablonický Juraj , Hanushchak-Yefimenko Liudmyla , Bukoros Tetiana 
Published: November 2023

Abstract
This article deals with the testing of regulating and non-regulating hydraulic pumps in laboratory conditions. The laboratory test was carried out on the hydraulic device consisting of two separate hydraulic circuits (for testing the non-regulating hydraulic pump and regulating hydraulic pump). A throttle valve or proportional throttle valve is used for loading. During the laboratory tests, the flow efficiency of the non-regulating hydraulic pump at rotation speed n = 1,500 rpm (nominal speed) was η
pp
= 98.91% (0 MPa) and η
pp
= 94.97% (20 MPa), and for the regulating hydraulic pump, the values were η
pp
= 97.57% (0 MPa) and η
pp
= 94.54% (20 MPa). The determined values of flow efficiency of hydraulic pumps determine the correct operation of the experimental hydraulic laboratory equipment.
The Prognostic Utility of Cytokines in Hospitalized COVID-19 Patients
Authors: Andrejkovits Ákos Vince , Huțanu Adina , Susányi Ervin József , Negrea Valentina , Văsieșiu Anca Meda 
Published: November 2023

Abstract

Introduction
The severity of COVID-19 relies on several factors, but the overproduction of pro-inflammatory cytokines remains a central mechanism. The aim of this study was to investigate the predictive utility of interleukin (IL)-6, IL-8, IL-10, IL-12, tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ) measurement in patients with COVID-19.

Material and Methods
We prospectively enrolled 181 adult patients with COVID-19 admitted to the 1st Infectious Disease County Hospital Târgu Mureș from December 2020 to September 2021. Serum cytokine levels were measured and correlated with disease severity, need for oxygen therapy, intensive care unit (ICU) transfer, and outcome.

Results
We found significantly higher serum levels of IL-6, IL-8, and IL-10 in patients with severe COVID-19 and in those with a fatal outcome. The logistic regression analysis showed a significant predictive value for IL-8 regarding disease severity, and for IL6 and IL-10 regarding ICU transfer and fatal outcome.

Conclusions
Serum levels of IL-6, IL-8, and IL-10 were significantly increased in patients with COVID-19, but their predictive value regarding disease severity and the need for oxygen therapy was poor. We found IL-6 and IL-10 to have a good predictive performance regarding ICU transfer and fatal outcome.
Monthly stream temperatures along the Danube River: Statistical analysis and predictive modelling with incremental climate change scenarios
Authors: Pekárová Pavla , Bajtek Zbyněk , Pekár Ján , Výleta Roman , Bonacci Ognjen , Miklánek Pavol , Belz Jörg Uwe , Gorbachova Liudmyla 
Published: November 2023

Abstract
The aim of the study is to analyse changes and predict the course of mean monthly water temperatures of the Danube River at various locations for the future. The first part of the study involves conducting a statistical analysis of the annual and monthly average air temperatures, water temperatures, and discharges along the Danube River. The study examines long-term trends, changes in the trends, and multiannual variability in the time series. The second part of the study focuses on simulating the average monthly water temperatures using Seasonal Autoregressive Integrated Moving Average (SARIMA) models and nonlinear regression models (NonL), based on two RCP based incremental mean monthly air temperature scenarios. To assess the impact of future climate on stream temperatures, the historical long-term average of the monthly water temperature (1990–2020) was compared with scenarios S1 (2041–2070) and S2 (2071–2100). The simulation results from the two stochastic models, the SARIMA and NonL, showed that in scenario S1, the Danube River’s average monthly water temperature is projected to increase by 0.81/0.82°C (Passau), 0.55/0.71°C (Bratislava), and 0.68/0.56°C (Reni). In scenario S2, the models predict higher increases: 2.83/2.50°C (Passau), 2.06/2.46°C (Bratislava), and 2.52/1.90°C (Reni). Overall, the SARIMA model proved to be more stable and effective in simulating the increase in monthly water temperatures in the Danube River.
Impact of biochar particle size and feedstock type on hydro-physical properties of sandy soil
Authors: Botková Natália , Vitková Justína , Šurda Peter , Massas Ioannis , Zafeiriou Ioannis , Gaduš Ján , Rodrigues Francisco Cota , Borges Paulo Filipe Silva 
Published: November 2023

Abstract
Biochar, as an organic amendment, could positively change soil properties, especially soil with low organic matter and/or poor structure. Biochar application in sandy soil with low organic matter could be an effective tool for improving hydro-physical parameters of the soil economically and ecologically as well. The effect on bulk density, particle density, porosity, saturated hydraulic conductivity and available water content for plants of two biochar types applied at three different particle sizes in a sandy soil was examined. The results confirmed previous studies, showing decreased bulk density, particle density and saturated hydraulic conductivity and partially increased available water content for plants and porosity. Both biochar type and particle size affected the studied soil hydro-physical parameters. After analysis and comparison of two different types of biochar and three particle sizes, the most effective treatment for sandy soil was proved by the biochar produced from willow with the smallest particle size (<125 µm).
Using feature engineering and machine learning in FAO reference evapotranspiration estimation
Authors: Považanová Barbora , Čistý Milan , Bajtek Zbyněk 
Published: November 2023

AbstractThe authors of this study investigated the use of machine learning (ML) and feature engineering (FE) techniques to accurately determine FAO reference evapotranspiration (ETo) with a minimal number of climate variables being measured. The recommended techniques for areas with insufficient measurements are based solely on daily temperature readings. Various ML methods were tested to evaluate how sophisticated an ML algorithm is for this task necessary. The main emphasis was on feature engineering, which involves converting raw variables into inputs better suited for ML algorithms, resulting in improved results. FE methods for estimating evapotranspiration include approximations of clear-sky solar radiation based on altitude and Julian day, approximate relative humidity and wind velocity, a categorical month variable, and variables interactions. The authors confirmed that the ability of ML in such tasks is not solely dependent on choosing the suitable algorithm but also on this frequently ignored step. The results of computational experiments are presented, accompanied by a comparison of the proposed method against standardEToempiric equations. Machine learning methods, mainly due to the transformation of raw variables using FE, provided better results than traditional empirical methods and sophisticated ML algorithms without FE. In addition, the authors tested the applicability of the developed models in the broader area to evaluate the possibility of their generalizability. The potential of this approach to deliver improved predictions, reduced input requirements, and increased efficiency holds interesting promise for optimizing water management strategies, irrigation planning, and decision-making within the agricultural sector.
A comparative study of the antiarrhythmic activity of anxiolytics, benzodiazepine and benzimidazole derivatives in a model of electrical ventricular fibrillation
Authors: Столярук В.Н. , Вититнова М.Б. , Цорин И.Б. , Крыжановский С.А. , Середенин С.Б. 
Published: November 2023

Цель исследования – сравнительное изучение антиаритмической активности фабомотизола и производных бензодиазепина (феназепама и диазепама) на модели электрической фибрилляции желудочков сердца у крыс с интактным и денервированным миокардом. Методика. В экспериментах на интактных крысах и крысах с денервированным миокардом в сравнительном аспекте изучена антиаритмическая активность анксиолитиков – производных бензодиазепина (феназепама и диазепама) и бензимидазола (фабомотизола). Результаты. Показано, что феназепам (1,0 мг/кг, в/в) и фабомотизол (7,5 мг/кг, в/в) у интактных животных, в отличие от диазепама (0,5 и 1,0 мг/кг в/в), статистически значимо (р=0,028) повышают порог электрической фибрилляции желудочков сердца. У животных с денервированным миокардом только фабомотизол проявлял противофибрилляторную активность (p<0,05). Заключение. Таким образом, анксиолитики фабомотизол и феназепам проявляют выраженную антиаритмическую активность, однако феназепам реализует свою антиаритмическую активность преимущественно на уровне ЦНС, а фабомотизол – на уровне кардиомиоцитов.
Aim. To compare the antiarrhythmic activity of fabomotizole and benzodiazepine derivatives (phenazepam and diazepam) in electrical ventricular fibrillation modeled in rats with intact and denervated myocardium. Methods. In experiments on intact rats and rats with denervated myocardium, the antiarrhythmic activity of the anxiolytics, benzodiazepine (phenazepam and diazepam) and benzimidazole (fabomotizole) derivatives, was compared. Results. It was shown that phenazepam (1.0 mg/kg, i.v.) and fabomotizole (7.5 mg/kg, i.v.) administered to intact animals, in contrast to diazepam (0.5 and 1.0 mg/kg, i.v.), significantly (p=0.028) increased the electrical ventricular fibrillation threshold. In animals with denervated myocardium, only fabomotizole showed an antifibrillatory activity (p<0.05). Conclusion. It can be assumed that phenazepam implements its antiarrhythmic activity mainly at the level of the central nervous system whereas fabomotizole produces it at the level of the myocardium.
Expression of transient receptor potential channels on peripheral blood leukocytes of patients with chronic obstructive pulmonary disease
Authors: Naumov D. E. , Sugaylo I. Yu. , Kotova O. O. , Gassan D. A. , Gorchakova Y. G. , Sheludko E. G. 
Published: November 2023

It is known that transient receptor potential (TRP) channels are receptors for reactive oxygen species and components of cigarette smoke, and therefore may be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD).Aim: To investigate the expression of TRPV1, TRPV4, TRPM8 and TRPA1 channels on peripheral blood leukocytes of COPD patients and healthy individuals and to establish its possible relationships with inflammatory markers and lung function parameters.Methodology and Research Methods. 23 patients with COPD of varying severity and 17 individuals without bronchial obstruction were examined. The expression of TRP channels on monocytes, lymphocytes and granulocytes was determined by indirect flow cytometry. Plasma concentrations of cytokines were measured in a multiplex assay. Lung function parameters were assessed by spirometry.Results. Monocytes from COPD patients were characterized by increased expression of TRPV1 (98.8 (97.6; 99.6)% vs. 97.8 (96.3; 98.6)%, p = 0.03) and TRPA1 (99.2 (98.7; 99.8)% vs. 95.1 (94.2; 98.4)%, p = 0.003). In addition, COPD was associated with an increased ratio of TRPV4/TRPM8 expression on monocytes (0.66 (0.51, 0.90) vs. 0.27 (0.12, 0.70), p = 0.02) and granulocytes (0.97 (0.86; 0.92) vs. 0.29 (0.06; 0.89), p = 0.003). TRPV4 expression on monocytes showed correlation with CXCL10 level (ρ = 0.55, p = 0.01), and for TRPM8 inverse relationships were found with the concentrations of IL-6 (ρ = –0.56, p = 0.02) and IL-10 (ρ = –0.55, p = 0.02). We did not find any associations between the expression of TRP channels and lung function parameters.Conclusion. Increased expression of TRPV1 and TRPA1, as well as an increased TRPV4/TRPM8 ratio on peripheral blood monocytes of COPD patients may indicate the contribution of these receptors to the proinflammatory activation of the cells and indicate possible prospects of TRP channels pharmacological modulation for the purpose of the disease therapy.
Effect of dietary fiber-enriched brown rice crackers on suppressing elevation of blood glucose level
Authors: Suzuki Hiroshi , Watanabe Kenichi , Ikeda Izumi , Takeda Yasunaga , Hatta Mariko , Horikawa Chika , D’Avila Ferreira Efrem , Sijia Wu , Laymon Khin , Sone Hirohito 
Published: November 2023

Background: Rice crackers are composed of carbohydrates refined from rice and enjoyed as a snack in Japan. Most rice crackers are crafted from white rice, and the potential postprandial blood glucose rise following their consumption may pose a clinical concern. Brown rice contains more dietary fiber than white rice and has been reported to suppress elevations of blood glucose. Dietary fiber-enhanced brown rice crackers have a significantly higher dietary fiber content than regular brown rice crackers and can be expected to suppress blood glucose elevations more reliably.Methods: We conducted a crossover trial in humans using white rice crackers and dietary fiber-enhanced brown rice crackers to investigate the effect of dietary fiber-enhanced brown rice crackers on postprandial blood glucose elevations. Participants ingested the 100 g rice crackers with 200 mL water for 10 min, and blood was collected from the fingertip at 7 time points: baseline, and at 15, 30, 45, 60, 90, and 120 min after ingestion of the rice cracker. The primary outcome measure was the blood glucose levels, and the secondary outcome measure was the incremental area under curve of blood glucose.Results: Glucose levels and incremental areas under curve at 60 min and 120 min after intake were significantly lower with dietary fiber-enhanced brown rice crackers than with white rice crackers. Incremental area under curves at 60 min and 120 min after intake of rice crackers were also significantly lower in dietary fiber-enhanced brown rice crackers than white rice crackers.Conclusions: Ingesting dietary fiber-enhanced brown rice crackers instead of white rice crackers could be useful for achieving better glycemic control.Keywords: Brown rice crackers, Dietary fiber, Postprandial blood glucose
Comparative analysis of high-intensity interval training and traditional rehabilitation programs for accelerated recovery from musculoskeletal injuries
Authors: M Kamalakannan , Josyula Snigdha , S Jenifer Augustina , M Naveen , J Hariharan , J Vignesh , P Karthikeyan , Alagesan Jagatheesan 
Published: November 2023

BACKGROUND: Musculoskeletal injuries, such as strains, are prevalent across all age groups and have a substantial impact on daily functioning and quality of life. OBJECTIVE: To examine the effectiveness of high-intensity interval training (HIIT) with traditional rehabilitation programs on pain, range of motion (ROM), muscular strength, and functional changes in promoting accelerated recovery from musculoskeletal injuries. METHODS: A total of 80 participants (54 males, 26 females; mean age 35.6 years) with various musculoskeletal injuries were randomly assigned to either the HIIT group (n= 40) or the traditional rehabilitation group (n= 40). The HIIT group underwent a six-week supervised program, with three sessions per week. The traditional rehabilitation group followed a similar six-week program emphasizing low to moderate intensity exercises and traditional rehabilitation techniques. Outcome measures, including pain levels, ROM, muscular strength, and functional outcomes, were assessed pre- and post-intervention. RESULTS: Significant improvements were observed in both the HIIT and traditional rehabilitation groups. However, the HIIT group demonstrated superior outcomes. Participants in the HIIT group experienced a greater reduction in pain levels compared to the traditional rehabilitation group (mean visual analog scale (VAS) score decrease of 5.2 vs. 3.8, respectively, p< 0.05). Functional outcomes significantly favored the HIIT group, with participants achieving faster completion times in the Timed Up and Go test (mean reduction of 2.1 seconds vs. 1.5 seconds, respectively, p< 0.01) and longer distances in the Single Leg Hop test (mean increase of 32 cm vs. 25 cm, respectively, p< 0.05). CONCLUSION: HIIT showed superior effectiveness over traditional rehabilitation in accelerating recovery from musculoskeletal injuries, with greater pain reduction and improved functional outcomes. Incorporating HIIT into rehabilitation protocols may offer an efficient approach for expedited recovery and enhanced functional capacity.
RUSYA-UKRAYNA SAVAŞINDA ÜÇÜNCÜ DEVLETLERCE ALINAN ÖNLEMLER
Authors: ÇALIŞKAN Ayla 
Published: November 2023

Karşı önlem, uluslararası haksız fiil sahibi bir devleti uluslararası yükümlülüklerini yerine getirmeye yöneltmek için başvurulan bir zorlama yoludur. Karşı önlemin hukuka uygun olması için mağdur devlet tarafından haksız fiil sahibi devlete yönelik alınması ve ona borçlu olduğu yükümlülüklerin yerine getirilmemesi şeklinde uygulanması gerekmektedir.
Karşı önlemler ve başvuru koşulları, Uluslararası Hukuk Komisyonu’nun 2001 yılında kabul edilen “Devletlerin Uluslararası Hukuka Aykırı Fiillerinden Doğan Sorumluluğu”na ilişkin maddeler metninde detaylı olarak düzenlenmiştir. Ancak Metnin “mağdur devletten başka devletlerce alınan önlemler” başlıklı 54. maddesinde “karşı önlemler” yerine “hukuka uygun önlemler” ifadesine yer verildiğinden, üçüncü devletlerin, bir bütün olarak uluslararası topluluğa yönelik bir ihlal gerekçesiyle sorumlu devlete yönelik karşı önlem alıp alamayacağı halen tartışılan bir meseledir.
Bu makale, Rusya’nın Ukrayna’ya saldırısı üzerine Ukrayna dışındaki üçüncü devletlerce kararlaştırılıp uygulanan önlemlerin hukuki niteliği meselesi üzerine yoğunlaşmıştır. Çalışmada öncelikle, Rusya’nın Ukrayna’ya saldırıları üzerine AB üyesi devletler ile İsviçre, ABD, İngiltere, Kanada ve Avustralya tarafından bu fiillere cevaben alınan önlemler sıralanacak, ardından “bir bütün olarak uluslararası topluluğa yönelik bir yükümlülüğün ihlali” sebebiyle üçüncü devletlerce alınan önlemlerin hukuki niteliği tartışılacaktır.
Penerapan Aplikasi Laboratorium Untuk Meningkatkan Kualitas Layanan Di Puskesmas Gandus
Authors: Gustriansyah Rendra , Puspasari Shinta , Sanmorino Ahmad , Suhandi Nazori , Antony Fery 
Published: November 2023

Abstract: The Public Health Center (Puskesmas) is a driving center for health-oriented development, empowering societies and families, and first-level health service is responsible for improving the quality of its services by delivering relevant, fast, and targeted information to achieve its vision and mission. Especially during the Covid-19 pandemic some time ago, the role of the Puskesmas laboratory as the frontline of the first-level health service center in examining Covid-19 symptoms and carrying out vaccinations is essential. Therefore, this community service activity aims to implement a web-based laboratory application at the Gandus health center to assist laboratory staff in completing, collecting, processing, and presenting data in a more structured, easy-to-read, timely, and accurate manner. The stages of the activity include interviews, discussions, patient data collection and laboratory examinations, application installation, and socialization of its use. This activity can improve the quality of laboratory services and add value to the accreditation of the Puskesmas.Keywords: application; laboratory; Public Health Center; service Abstrak: Puskesmas sebagai pusat penggerak pembangunan berwawasan kesehatan, pemberdayaan masyarakat, keluarga dan tempat pelayanan kesehatan tingkat pertama bertanggung jawab meningkatkan mutu layanannya melalui penyampaian informasi yang relevan, cepat dan tepat sasaran kepada pasien, dalam upaya mencapai visi dan misinya. Terutama di saat pandemi Covid-19 beberapa waktu yang lalu, maka peran laboratorium di puskesmas sebagai garda terdepan pusat layanan kesehatan tingkat pertama dalam pemeriksaan gejala Covid-19 dan pelaksanaan vaksinasi menjadi penting. Oleh karena itu, kegiatan pengabdian kepada masyarakat ini bertujuan untuk menerapkan aplikasi laboratorium berbasis web di puskesmas Gandus dalam upaya untuk membantu staf laboratorium dalam melengkapi, mengumpulkan, memproses, dan mempresentasikan data secara lebih terstruktur, mudah dipahami, akurat, dan tepat waktu. Tahapan kegiatan meliputi wawancara, diskusi, pengambilan data pasien dan pemeriksaan laboratorium, instalasi aplikasi, serta sosialisasi penggunaannya. Kegiatan ini dapat berkontribusi untuk meningkatkan kualitas layanan laboratorium dan menambah nilai akreditasi puskesmas.Kata kunci: aplikasi; laboratorium; layanan; puskesmas