bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced activity of an immunogenic macromolecule Amylase Trypsin inhibitor (ATI) in wheat through CRISPR/Cas9 mediated multiple gene editing
Authors: Phogat Sachin , Poudel Ankur , Gayatri  , Kaushik Megha , Madhavan Jayanthi , Sevanthi Amitha Mithra , Padaria Jasdeep Chatrath , Nekrasov Vladimir , Singh Pradeep Kumar , Mandal Pranab Kumar 
Published: January 2025

Abstract

Wheat is the staple food for 40% of the world, providing 20% of dietary energy and protein. However, along with providing nutrition, wheat contains several anti-nutritional macromolecules. Amylase/Trypsin inhibitors (ATIs) are one such macromolecular proteins which have been known to cause allergic reactions like baker's asthma, auto-immunogenic reactions like Non-Celiac Wheat Sensitivity, and primary triggers for Celiac Disease in some predisposed humans. Bread wheat varieties without ATI molecules or with reduced activity have not yet been developed. Here, multiple genes of major ATI protein molecules were mutated using tRNA-based multiplex CRISPR/Cas9 genome editing technology. ATI proteins were extracted from wheat flours of gene-edited wheat lines along with unedited plants and subjected to quantification, detection by SDS-PAGE, fractionation by HPLC, and assayed the α-amylase and trypsin inhibition activity. Gene-edited Bobwhite wheat plant produced seeds with reduced (up to 30.61%) ATI content, which resulted in a reduction in α-amylase and trypsin inhibition activity to 50.74% and 44.90%, respectively. Another variety of bread wheat HD2967 also showed a significant reduction in ATIs content as well as a reduction in α-amylase and trypsin inhibition activity. This result suggests the possibility of developing low immunogenic wheat lines by multiple gene editing for the immunogenic macromolecules.
Predicting sentinel lymph node metastasis in breast cancer: A study based on the SEER database
Authors: Li Qingyang , Xu Hu , Bao Baoshi , Xie Yujiao , Guo Shiqi , Gao Zhaofeng , Chen Siyi , Sun Jiahong , Zhu Li , Wang Jiandong 
Published: January 2025

Abstract

Background:Sentinel lymph node biopsy (SLNB), a standard surgical procedure for clinically axillary-negative breast cancer patients, significantly reduces complications compared with axillary lymph node dissection, but it’s still a relatively invasive procedure with some complications, affecting patient’s quality of life. To identify patients who might benefit from avoiding SLNB, this study aimed to develop a nomogram for predicting sentinel lymph node metastasis (SLNM) in breast cancer patients using the SEER database.
Methods: We identified breast cancer patients whose 1-5 lymph nodes were examined in the SEER database as those who underwent SLNB. Patients were randomly assigned to the training and validation cohorts at a 3:1 ratio. Univariate and multivariate logistic regression were used to evaluate the relationships between SLNM and patients' clinicopathological characteristics. A nomogram was constructed, and its performance was validated via ROC curves, calibration curves, and decision curve analysis.
Results:Age, race, primary site, T stage, M stage, histological grade, pathological type, oestrogen receptor status, and progesterone receptor status are independent predictive factors for SLNM in patients with breast cancer. We successfully developed a predictive nomogram for sentinel lymph node status, with AUC values of 0.711 and 0.700 for the training and validation cohorts, respectively.
Conclusion: Our study successfully established an SLNM nomogram that provides richer predictive information. The model exhibits good clinical efficacy and can provide reference value for populations potentially exempt from SLNB.
β-Cyclodextrin-based Stimuli-Responsive Nanogels for Gymnema sylvestre: Therapeutic Potential and Safety evaluation in Diabetic conditions
Authors: Rahman Atiq Ur , Minhas Muhammad Usman , Sohail Muhammad 
Published: January 2025

Abstract

Historically, herbal medicines have played a significant role in treating various illnesses. Specifically concerning diabetes, numerous plant-based remedies have been utilized to regulate blood sugar levels and improve insulin sensitivity. However, despite their popularity, many herbal medicines have poor solubility, leading to low bioavailability and the need for higher or repeated dosing, which can limit their therapeutic viability. Gymnema sylvestre, a well-known herb with a history in diabetes management, contains gymnemic acids face bioavailability challenges due to limited solubility, impacting it clinical efficacy. This study focuses on enhancing Gymnema sylvestre’s solubility and bioavailability using stimuli responsive nanogels with β-Cyclodextrin, cross-linked with Methacrylic Acids (MAA) through free radical polymerization. The optimized formulation NGT4, showed favorable characteristics: high drug entrapment, increased solubility, and a particle size of 201nm. Analytical methods like FTIR, TGA/DSC, SEM, and PXRD confirmed the successful polymeric network and stable nanogel properties. In vitro release studies in acidic and basic media demonstrate controlled drug release, while in vivo tests confirmed the nanogel’s safety, biocompatibility, and effective hypoglycemic effects. These findings suggest that pH-responsive nanogels are promising approach to improve the solubility and therapeutic performance of Gymnema sylvestre in diabetes management.
Success Factors and Measures for Scaling Patient-Facing Digital Health Technologies from Leaders Insights
Authors: Pfitzer Estelle , Giger Odile-Florence , Kausch Christoph , Kowatsch Tobias 
Published: January 2025

Abstract

Background
Europe’s healthcare systems face a triple burden: the rise of non-communicable diseases (NCDs), an aging population, and a shortage of healthcare professionals. NCDs, the leading causes of death, disproportionately affect older adults, placing significant pressure on healthcare services. By 2050, nearly 30% of Europe’s population will be aged 65 or older, up from 20% in 2023. These challenges demand urgent solutions to sustain healthcare systems. Patient-facing digital health technologies (DHTs), such as Digital Diagnostics and Digital Therapeutics, offer promising tools to address this burden by empowering patient self-management, reducing strain on healthcare professionals, and enhancing system efficiency. Despite their potential, the scaling and adoption of DHTs remain limited. This study investigates: (RQ1) What key factors drive success across different patient-facing DHT categories? and (RQ2) How can companies implement these factors?
Methods
Following COREQ guidelines, we conducted semi-structured interviews with 29 executives and founders of European DHT companies targeting NCDs. Participants were identified using PitchBook, focusing on revenue-generating companies with over 20 employees. Virtual interviews were conducted in English between May and September 2024, lasting an average of 28 minutes (range: 21–40). Data saturation determined the sample size. Thematic analysis was performed, with two researchers independently coding the data to ensure reliability. Success factors were categorized as internal (e.g., employees) or external (e.g., partnerships). Ethical approval was obtained, and data was anonymized.
Results
We identified 19 success factors for scaling patient-facing DHTs. Health & Wellness companies prioritized business model flexibility (80%), while Digital Therapeutics relied on regulatory compliance (100%). Validation of health impact was critical across categories, emphasized by all respondents in Digital Diagnostics and Digital Therapeutics. Other key factors included customer awareness, strategic partnerships, and investor alignment, highlighting the importance of tailored growth strategies.
Conclusion
This study provides a structured framework for scaling patient-facing DHTs, emphasizing category-specific strategies aligned with operational, regulatory, and consumer demands. It offers actionable guidance for founders and executives to allocate resources effectively and adapt to diverse market contexts. By addressing the unique challenges of scaling DHTs, this work contributes to advancing digital health research and improving healthcare system resilience.
Personalized dose reduction strategies for biologic disease-modifying antirheumatic drugs for treating ankylosing spondylitis: a clinical and economic evaluation with predictive modeling
Authors: Bui Hai Binh , Nguyen Thi Thu Phuong , Vu Thi Thanh Hang , Ngo Thi Thuc Nhan , Nguyen Thi Nhu Hoa , Hoang Dung Van 
Published: January 2025

Abstract

Background
Ankylosing spondylitis (AS) is a chronic inflammatory disease that significantly affects quality of life and imposes a high economic burden on patients due to the cost of biologic disease-modifying antirheumatic drugs (bDMARDs). Dose reduction strategies for bDMARDs may offer a feasible approach to maintaining clinical efficacy while reducing costs. This study aimed to evaluate the clinical effectiveness and cost-efficiency of bDMARD dose reduction in patients with AS and apply predictive modeling to identify key factors influencing disease control.
Methods
This 12-month prospective study included 368 patients with AS who were divided into two groups: those who received dose reduction and those with full-dose therapy. Clinical outcomes such as C-reactive protein (CRP) levels, the Bath ankylosing spondylitis disease activity index (BASDAI) and ankylosing spondylitis disease activity score (ASDAS) were assessed, along with cost effectiveness using incremental cost effectiveness ratios (ICER). Random forest models were developed to predict the achievement of inactive disease (ASDAS < 1.3) and to identify key predictors.
Results
The ICER to achieve an ASDAS < 1.3 was highly favorable (-$16,772.62). Patients in the dose reduction group demonstrated significant improvements in CRP levels (-4.65 vs. -1.32 mg/L, p < 0.001), BASDAI (-3.00 vs. -0.42, p < 0.001), and ASDAS (-1.72 vs. -0.15, p < 0.001), compared with the full dose group. Predictive modeling identified baseline CRP level, baseline ASDAS, and dose adjustment as key factors influencing outcomes, with the medium feature model achieving an area under the receiver operating characteristic curve of 81.86%.
Conclusions
The reduction in bDMARD doses maintained clinical efficacy and achieved significant cost savings, offering a viable strategy for the management of AS. Predictive modeling provided actionable insights to optimize personalized treatment strategies, balancing efficacy and economic sustainability. These findings support the integration of dose reduction strategies into routine practice, particularly in resource-limited settings.
Brain stem glioma surgery via a telovelar approach using sodium fluorescein in a 6-year-old pediatric patient
Authors: Cavalheiro Sergio , Quiróz José Antonio Rangel , Dastoli Patricia Alessandra , Kim Isaque Hyung Tong , Suzuki Fernando Seiji , Costa Marcos Devanir Silva da 
Published: January 2025

Abstract

Tectal gliomas are a subset of brainstem gliomas that typically present symptoms related to hydrocephalus due to obstruction of cerebrospinal fluid pathways. These tumors are generally low grade and have a favorable prognosis.
Management often focuses on resolving hydrocephalus, with surgical intervention considered when symptoms worsen.
Sodium fluorescein is a fluorescent dye used in brain tumor surgeries to enhance visualization, allowing surgeons to distinguish between tumor tissue and normal brain tissue more effectively. This technique improves the precision and safety of tumor resection.
We present the case of a 6-year-old patient in whom we performed the telovelar approach to resect the exophytic portion at the level of the Sylvian aqueduct via sodium fluorescein (2.5 mg/kg) for adequate resection, with excellent postoperative results.
Ethics committee approval was not required to perform the surgical procedure on the patient. The patient's parents provided informed consent for the surgery and gave verbal consent for the publication of their video surgery.
Kolonialisme, Perekrutan Militer, dan Transformasi Sosial: Pengaruh Belanda di Minahasa
Authors: Andani Devi , Saefudin Arif 
Published: January 2025

The Dutch came to Minahasa land to exploit the fertile Minahasa land with various natural resources, but there was a pattern of social change that occurred between the Dutch who were successful in "making friends" with the Minahasa people. This slowly changed the structure of Minahasa society. So Minahasa is an area famous for having the largest and longest Dutch influence in Indonesian history. This article uses library research methods related to critical historical methods. The steps in this research are associated with using historical research methods which include : heuristics, criticism, interpretation and historiography. The Dutch saw a lot of instability and rebellion in several areas that occurred in controlling the Dutch East Indies government and took the initiative to recruit military members again in a more intensive and selective way. The recruitment process for Dutch East Indies military members carried out in Minahasa was not followed by a rejection process. The presence of the Dutch in the lives of the Minahasan people created stereotypes for the people during colonialism. This certainly creates a paradigm for people's thinking during colonialism. The results of the reconstruction of the thoughts of the Minahasa people who had united with the Dutch certainly gave rise to resistance and disagreement regarding this friendly relationship.
Implementasi Program Pembelajaran Tahfidz Dalam Meningkatkan Motivasi Belajar Al-Qur'an Santri Di Pondok Pesantren Al-Mubarok Pajo Nusa Tenggara Barat
Authors: Mu’iz Abd 
Published: January 2025

Penelitian ini bertujuan untuk mengetahui Implementasi Program Pembelajaran Tahfidz Dalam Meningkatkan Motivasi Belajar Al-Qur'an Santri Di Pondok Pesantren Al-Mubarok Pajo Nusa Tenggara Barat. Penelitian ini memfokuskan penulis untuk meneliti latar belakang, interaksi dan kondisi lingkungan tertentu (dalam hal ini adalah Pondok Pesantren Al-Mubarok Pajo Nusa Tenggara Barat). Dalam Meningkatkan Motivasi Belajar Al-Qur'an Santri Di Pondok Pesantren Al-Mubarok Pajo Nusa Tenggara Barat, maka dapat peneliti simpulkan: Motivasi belajar santri pada program Tahfidz Al-Quran di pondok pesantren Al-Mubarok Pajo-NTB. Secara garis besar motivasi santri dalam menghafal Al-Qur’an terdiri dari dua jenis motivasi yaitu Motivasi Intrinsik dan motivasi ekstrinsik, Implementasi program Tahfidz Al-Quran dalam meningkatkan motivasi belajar santri di pondok pesantren Al-Mubarok Pajo- NTB. Pondok pesantren Al-Mubarok Pajo NTB sudah menerapkan pembelajaran tahfidz Al-Qur’an sejak tahun 2018. Semua siswa yang berasrama diwajibkan untuk Menghafal Al-Qur’an sesuai dengan program Tahfidz yang ada di pondok pesantren tersebut. Dan target untuk santri selama masa sekolah 3-5 Juz. Dan dalam mengimplementasikan pembelajaran tahfidz ada tiga tahapan yang dilalui yaitu tahap perencanaan, pelakasanaan dan evaluasi. Pada pelaksanaan pembelajaran Tahfidz Al-Qur’an ini sudah berjalan cukup baik, namun perlu adanya peningkatan agar suatu tujuan yang diharapkan dapat tercapai secara optimal. Hal ini dapat terlihat dari hasil observasi, wawancara serta dokumen yang peneliti dapatkan dari lapangan dan Faktor pendukung dan penghambat dalam meningkatkan motivasi belajar Al-Quran santri di pondok pesantren Al-Mubarok Pajo-NTB.
Admission trends, associated factors and the outcomes for children hospitalised to paediatric intensive care unit for asthma – A population based longitudinal study
Authors: Krishnamurthy Kandamaran , King Reginald , Oredein Babatunde , Kumar Alok 
Published: January 2025

Objectives:
This study aims to quantify the burden of pediatric intensive care unit (PICU) admissions from acute severe asthma and to describe the associated factors and outcome in these admissions.

Material and Methods:
This was a prospective longitudinal population based study from 2015 through 2019. Included all children (< 16 years) with acute severe asthma who needed hospitalization. Ethical approval for the data collection was obtained. Real time hospital admission date was collected by one of the authors. The medical records of the patients were examined as per the objectives of the study.

Results:
From among the 13407 Emergency Room visits from acute asthma during study period, there were 1748 (13.0%) required hospital admissions and 101 (0.8%) including 66 (65.3%) males and 35 (34.7%) females were admitted to the PICU. Of the PICU admissions, 76 (75.2%) and 47 (46.5%) had previous hospitalization and PICU admissions respectively. Among those admitted to PICU 29 (28.7%), 61 (60.4%) and 11 (10.9%) were on daily inhaled corticosteroids (ISC), were non-compliant with their inhaled corticosteroids (ICS) and were not on any ICS. There was no mortality from asthma during the study period.

Conclusions:
The majority of the PICU admissions for acute asthma was among children with previous asthma hospitalization and those who did not use ICS or did not comply with ICS.
Diagnostic value of flexible fibre optic bronchoscopy in the evaluation of children with suspected airway foreign body: A retrospective study
Authors: Khandelwal Shyam Madanlal , Khadke Rajendra Raghunath , Joshi Abhijit Venkatesh 
Published: January 2025

Objectives:
To evaluate the role of flexible fibre optic bronchoscopy in the diagnosis of suspected airway foreign bodies in children.

Material and Methods:
A retrospective study of suspected airway foreign bodies in 124 cases in children was done.

Results:
Results were obtained in terms of percentage of cases in which foreign body was seen, age incidence, sex difference, type of foreign body, history, bronchoscopy findings and findings of other relevant radiological investigations and data compiled and compared with some other retrospective studies

Conclusion:
The negative predictive value of a diagnostic bronchoscopy in viewing a foreign body is high and can also guide in finding other causes for the obstruction. It also provides an opportunity for other procedures, such as tracheo-bronchial lavage and suctioning of secretions, in addition to removal of foreign bodies in some.
Clinico-etiological profile of haematuria in children: A retrospective analysis
Authors: Ashiq Mohammed , Sahana K. S. 
Published: January 2025

Objectives:
The objectives of this study were to study demographic, etiological and clinical profiles of children presenting with haematuria.

Material and Methods:
This was a retrospective hospital-based study conducted by referring medical records of patients (aged 1 month–16 years) admitted to Yenepoya Medical College, Mangalore, who were treated for haematuria between January 2018 and December 2022.

Results:
Of the 84 children (40 males, mean age 7.3 ± 4.94 years) who presented with haematuria, 48 were microscopic, 36 were with gross haematuria, and 56 were with non-glomerular, 28 were with glomerular haematuria. Common causes included urinary tract infections (47%), post-infectious glomerulonephritis (22.6%), renal calculi and hydronephrosis. Deranged renal function (17.6%) and dyselectrolytemia (53.5%) were frequent complications. Anaemia (61.9%) was the most prevalent associated comorbidity. Acute renal failure occurred in 7.14% and mortality in 2.4%. Glomerular haematuria was associated with hypertension, oliguria and oedema.

Conclusion:
This study highlights the diverse aetiologies of haematuria in children, emphasising the need for a comprehensive evaluation and prompt management to prevent long-term renal damage. The findings inform clinical practice and guide further research into paediatric haematuria. The distinction between glomerular and non-glomerular aetiology will help in the proper investigation of the child and further aid in effective management.
RANCANG BANGUN SISTEM INFORMASI PENGELOLAAN DATA KARYAWAN ROYAL LAUNDRY BERBASIS WEB MENGGUNAKAN METODE KANBAN
Authors: Altalariksyah Faiz , Mutiah Nurul , Febriyanto Ferdy 
Published: January 2025

Perkembangan sistem informasi telah berdampak pada banyak aspek kehidupan manusia dalam melakukan aktivitas harian. Banyak sektor, termasuk dunia bisnis, melihat efek positif dari penerapan sistem informasi. Salah satu bisnis yang bisa didukung oleh teknologi informasi adalah bisnis laundry. Royal laundry adalah sebuah tempat usaha laundry yang berlokasi di kota Pontianak yang mempunyai 24 pegawai. Untuk sistem operasional seperti pelayanan presensi, izin cuti, dan penggajian masih manual. Sistem manual ini menggunakan pencatatan melalui kertas ketika presensi, pembuatan slip gaji, hingga rekapitulasi bulanan dan cuti. Hal tersebut berdampak pada sulitnya pegawai untuk izin cuti, sistem penggajian yang manual sehingga menyulitkan dari segi pencatatan. Berdasarkan permasalahan tersebut, dirancang sebuah sistem informasi penggajian menggunakan metode Kanban. Kanban digunakan karena memiliki alur pengembangan yang cepat dan efektif dalam pembangunan sistem. Hasil dari penelitian ini adalah sistem informasi presensi dan penggajian berbasis web yang diujikan menggunakan black-box dan skala likert. Hasil dari uji fungsionalitas sistem yang diuji oleh pihak royal laundry menunjukkan bahwa sistem informasi presensi dan penggajian karyawan di royal laundry memiliki nilai yang sangat baik dan diterima oleh pemilik dari royal laundry. Hal ini menunjukkan bahwa sistem informasi yang dibuat dapat membantu memecahkan masalah yang dialami oleh usaha royal laundry dalam melakukan manajemen karyawan.
Analisis Kepuasan Pengguna Aplikasi Halodoc Menggunakan Metode End User Computing Satisfaction (EUCS) dan Importance Performance Analysis (IPA) (Studi Kasus : Pengguna Aplikasi Halodoc di Kota Pontianak)
Authors: Bella Nur Aisyah , Mutiah Nurul , Rahmayuda Syahru 
Published: January 2025

Penggunaan aplikasi kesehatan digital terus berkembang di Indonesia untuk meningkatkan akses layanan kesehatan yang lebih praktis dan efisien. Salah satu aplikasi yang cukup populer adalah Halodoc, yang memungkinkan pengguna berkonsultasi dengan dokter, memesan obat, dan melakukan tes kesehatan secara online. Aplikasi ini telah menjadi solusi bagi masyarakat untuk memenuhi kebutuhan layanan kesehatan digital. Namun, masih ada keluhan terkait performa aplikasi, seperti ketepatan waktu layanan dan kemudahan penggunaan yang dirasa belum optimal. Oleh karena itu, penilaian terhadap kualitas layanan Halodoc menjadi penting. Studi ini menggunakan metode End User Computing Satisfaction (EUCS) dan Importance Performance Analysis (IPA) untuk menganalisis kinerja layanan berdasarkan pengalaman pengguna. Dimensi yang diukur meliputi Content, Accuracy, Format, Ease of Use, dan Timeliness. Data diperoleh dari kuesioner yang disebarkan kepada 100 pengguna. Hasil analisis menunjukkan nilai rata-rata kinerja (performance) sebesar 4,23 dan tingkat kepentingan (importance) sebesar 4,43. Nilai rata-rata tingkat kesesuaian adalah 95%, dengan kesenjangan (gap) sebesar -0,20 yang menunjukkan bahwa kinerja layanan Halodoc di Kota Pontianak perlu ditingkatkan. Berdasarkan analisis kuadran IPA, fokus utama perbaikan berada pada kuadran I, yaitu variabel Accuracy dan Timeliness. Kata kunci: Kepuasan Pengguna, Halodoc, End User Computing Satisfaction, Importance Performance Analysis