bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beneficial effect of nitric oxide synthase inhibitor on hepatotoxicity induced by allyl alcohol
Authors: Alam Khurshid , Nagi Mahmoud N. , Al‐Shabanah Othman A. , Al‐Bekairi Abdullah M. 
Published: August 2002

AbstractThe effect of aminoguanidine (a selective inhibitor of inducible nitric oxide synthase) on allyl alcohol‐induced liver injury was assessed by the measurement of serum ALT and AST activities and histo‐ pathological examination. When aminoguanidine (50–300 mg/kg, i.p.) was administered to mice 30 min before a toxic dose of allyl alcohol (75 μL/kg, i.p.), significant changes related to liver injury were observed. In the presence of aminoguanidine the level of ALT and AST enzymes were significantly decreased. All symptoms of liver necrosis produced by allyl alcohol toxicity almost completely disappeared when animals were pretreated with aminoguanidine at 300 mg/kg. Depletion of hepatic glutathione as a consequence of allyl alcohol metabolism was minimal in mice pretreated with aminoguanidine at 300 mg/kg. It was found that the inhibition of toxicity was not due to alteration in allyl alcohol metabolism since aminoguanidine did not effect alcohol dehydrogenase activity both in vivo and in vitro. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:317–321, 2001
TCDD‐mediated oxidative stress in male rat pups following perinatal exposure
Authors: Slezak B. P. , Hamm J. T. , Reyna J. , Hurst C. H. , Birnbaum L. S. 
Published: August 2002

Abstract2,3,7,8‐Tetrachlorododibenzo‐p‐dioxin (TCDD) is a highly persistent trace environmental contaminant and is one of the most potent toxicants known. Exposure to TCDD has been shown to cause oxidative stress in a variety of animal models. In this study, pregnant Long Evans rats were dosed with 1 μg TCDD/kg on gestational day (GD) 15 so as to investigate oxidative stress in the liver of male pups following gestational exposure to TCDD. Lipid peroxidation (TBARS), production of reactive oxygen species (ROS), and total glutathione (GSH) were assayed to identify changes in oxidative stress parameters in the pup liver at GD 21 and postnatal days (PND) 4, 25, 32, 49, and 63. Mean ROS levels in pups were elevated at all time points tested with a significant elevation at PND 4 and PND 25. However, pup hepatic lipid peroxidation was unchanged throughout the time course. In addition, hepatic total GSH levels were not significantly changed although the means for the TCDD‐treated groups were less than those of the controls at all time points except PND 49. The results indicate that although the levels of ROS are increased following gestational/lactational exposure, this increase does not translate to direct oxidative damage or significant changes to endogenous antioxidant defense mechanisms. Further investigation into the effect of gestational/lactational exposure in pups should include additional endpoints for further characterization of the time course of the response, the effect upon extrahepatic tissues, and investigation of differences between male and female offspring. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:49–52, 2002; Published online in Wiley Interscience (www.interscience.wiley.com). DOI 10.1002/jbt.10024
Why Is “Integrated” ABC Better?
Authors: Keys David E. , LeFevre Robert J. 
Published: August 2002

AbstractTraditionally, the various parts of an organization have been assumed to be independent. This was done mainly to
simplify calculations, though the assumption is almost always inaccurate in reality. But computer technology now
makes it possible to account for the many interrelationships of activities. The explicit consideration of these
interrelationships in product and service costing can be referred to as integrated activity‐based costing. By
measuring these interrelationships, managers can better understand the parts of an organization, how they relate
with each other, and how they can be integrated into the entire organization. © 2002 Wiley Periodicals,
Inc.
Intangible Asset Accounting: How Do Worldwide Rules Differ?
Authors: Bean LuAnn , Jarnagin Bill D. 
Published: August 2002

AbstractThe quest to account for intangible assets has sparked debate both here and abroad. With so many
cross‐border and domestic U.S. mergers, what do the latest changes mean for today's financial
executive? © 2001 John Wiley & Sons, Inc.
Southeast asia: The next M&A hotspot?
Authors: Metwalli Ali M. , Tang Roger Y.W. 
Published: August 2002

AbstractMany analysts believe Southeast Asia could be the site of an economic boom in the coming years. But what has
been happening with M&A activity? And will expansion continue? The authors take a detailed look
at several countries in the region, and offer some practical suggestions for potential investors.
© 2002 John Wiley & Sons, Inc.
Direct effect of a gonadotropin‐releasing hormone agonist on the growth of canine mammary tumour cells
Authors: Pagnini Ugo , Florio Salvatore , Crispino Luca , Pagnini Giuseppe , Colangelo Donato , Rocco Daniela , Pacilio Carmen , Pacilio Maria , Macaluso Marcella , Giordano Antonio 
Published: August 2002

AbstractGonadotropin‐releasing hormone (GnRH) agonist exert “in vivo” an inhibitory action on the growth of hormone‐dependent canine mammary tumours (Lombardi et al. [1999] J. Vet. Pharmacol Ther. 22(1):56–61). The present experiments have been performed “in vitro” in order to investigate the mechanisms involved in this direct antiproliferative action of GnRH agonists. In particular, the aim was to study whether these compounds might exert their antiproliferative effect by interfering with the stimulatory action of epidermal growth factor (EGF). To this purpose, the effects of GnRH agonist, Goserelin (GnRH‐A), on the mitogenic action of EGF, on EGF‐activated intracellular signaling mechanisms (intracellular calcium and nitric oxide production) as well as on ATP induced cell proliferation and signalling, and on the binding of EGF receptors have been evaluated in primary culture of canine mammary tumour cells. The results of these “in vitro” studies show that GnRH‐A counteracts the mitogenic action of EGF and ATP, decreases the EGF/ATP‐induced calcium signalling and reduces EGF binding, probably by means of NO‐induced [Ca2+]idownregulation. These data suggest that GnRH agonists may inhibit the proliferation of the tumour cells by interfering with the stimulatory action of EGF. J. Cell. Biochem. 85: 470–481, 2002. © 2002 Wiley‐Liss, Inc.
Single‐stranded nucleic acid‐binding protein, Purα, interacts with RNA homologous to 18S ribosomal RNA and inhibits translation in vitro
Authors: Gallia Gary L. , Darbinian Nune , Jaffe Neil , Khalili Kamel 
Published: August 2002

AbstractPurα is a highly conserved, eukaryotic sequence‐specific DNA‐ and RNA‐binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. Purα exerts its activity in part by interacting with other viral and cellular proteins. One such protein is the human immunodeficiency virus (HIV) type I regulatory protein Tat. Earlier studies have demonstrated that this interaction is mediated by Purα‐associated RNA (PARNA) and that RNA immunopurified from mammalian expressed Purα was capable of reconstituting the interaction between these two proteins. In the current study, we characterize four RNA species which were immunopurified with Purα. Northern blot analysis with one of the PARNAs revealed a highly abundant signal of ∼2.0 kilobases (kb) present in all cell lines tested. Sequence analysis of each of the four PARNA clones revealed a high homology to different regions of the human 18S ribosomal RNA sequence. Based on this homology, we investigated the influence of Purα on translation. Luciferase assays were performed after coupled in vitro transcription/translation reactions with a vector containing a luciferase reporter construct and increasing concentrations of BSA, GST, and GST‐Purα. Inclusion of GST‐Purα in these reactions resulted in a dose‐dependent inhibition of luciferase activity. Similar inhibition was observed with in vitro translation reactions performed with in vitro transcribed luciferase RNA and increasing concentrations of GST‐Purα. In control experiments, inclusion of increasing concentrations of GST‐Purα with luciferase protein resulted in no effect on luciferase activity. Taken together, these data demonstrate that Purα inhibits translation reactions in vitro. Moreover, this Purα‐mediated inhibition of translation can be abrogated by HIV‐1 Tat protein. J. Cell. Biochem. 83: 355–363, 2001. © 2001 Wiley‐Liss, Inc.
Analysis of altered gene expression in rat soleus muscle atrophied by disuse
Authors: Cros Nathalie , Tkatchenko Andrei V. , Pisani Didier F. , Leclerc Lilian , Léger Jean J. , Marini Jean‐François , Dechesne Claude A. 
Published: August 2002

AbstractThe present study involved a global analysis of genes whose expression was modified in rat soleus muscle atrophied after hindlimb suspension (HS). HS muscle unloading is a common model for muscle disuse that especially affects antigravity slow‐twitch muscles such as the soleus muscle. A cDNA cloning strategy, based on suppression subtractive hybridization technology, led to the construction of two normalized soleus muscle cDNA libraries that were subtracted in opposite directions, i.e., atrophied soleus muscle cDNAs subtracted by control cDNAs and vice versa. Differential screening of the two libraries revealed 34 genes with altered expression in HS soleus muscle, including 11 novel cDNAs, in addition to the 2X and 2B myosin heavy chain genes expressed only in soleus muscles after HS. Gene up‐ and down‐regulations were quantified by reverse Northern blot and classical Northern blot analysis. The 25 genes with known functions fell into seven important functional categories. The homogeneity of gene alterations within each category gave several clues for unraveling the interplay of cellular events implied in the muscle atrophy phenotype. In particular, our results indicate that modulations in slow‐ and fast‐twitch‐muscle component balance, the protein synthesis/secretion pathway, and the extracellular matrix/cytoskeleton axis are likely to be key molecular mechanisms of muscle atrophy. In addition, the cloning of novel cDNAs underlined the efficiency of the chosen technical approach and gave novel possibilities to further decipher the molecular mechanisms of muscle atrophy. J. Cell. Biochem. 83: 508–519, 2001. © 2001 Wiley‐Liss, Inc.
New approach to free energy of solvation applying continuum models to molecular dynamics simulation
Authors: Gonçalves Paulo F. B. , Stassen Hubert 
Published: August 2002

AbstractA new approach to the calculation of the free energy of solvation from trajectories obtained by molecular dynamics simulation is presented. The free energy of solvation is computed as the sum of three contributions originated at the cavitation of the solute by the solvent, the solute‐solvent nonpolar (repulsion and dispersion) interactions, and the electrostatic solvation of the solute. The electrostatic term is calculated based on ideas developed for the broadly used continuum models, the cavitational contribution from the excluded volume by the Claverie‐Pierotti model, and the Van der Waals term directly from the molecular dynamics simulation. The proposed model is tested for diluted aqueous solutions of simple molecules containing a variety of chemically important functions: methanol, methylamine, water, methanethiol, and dichloromethane. These solutions were treated by molecular dynamics simulations using SPC/E water and the OPLS force field for the organic molecules. Obtained free energies of solvation are in very good agreement with experimental data. © 2002 Wiley Periodicals, Inc. J Comput Chem 23: 706–714, 2002
Equilibrium inversion barrier of NH3 from extrapolated coupled‐cluster pair energies
Authors: Klopper Wim , Samson Claire C. M. , Tarczay György , Császár Attila G. 
Published: August 2002

AbstractThe basis‐set convergence of singlet and triplet pair energies of coupled‐cluster theory including single and double excitations is accelerated by means of extrapolations based on the distinct convergence behaviors of these pairs. The new extrapolation procedure predicts a nonrelativistic Born–Oppenheimer inversion barrier of 1767±12 cm−1 for NH3. An effective one‐dimensional, vibrationally averaged barrier of 2021±20 cm−1 is obtained when relativistic effects (+20 cm−1), Born–Oppenheimer diagonal corrections (−10 cm−1), and zero‐point vibrations (+244 cm−1) are accounted for. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 1306–1314, 2001
Pitch tournament designs and other BIBDs—existence results for the case ν = 8n
Authors: Abel R. Julian R. , Finizio Norman J. , Greig Malcolm , Lewis Scott J. 
Published: August 2002

AbstractA pitch tournament is a resolvable or near resolvable(ν,8,7) BIBD that satisfies certain criteria in addition to theusual condition that ν ≡ 0 or 1 (mod 8). Here we establish that for the case ν = 8n the necessary condition forpitch tournaments is sufficient for all n > 1615, with at most 187 smaller exceptions. This complements our earlier study of the ν = 8n + 1 case, where we established sufficiency for all n > 224, with at most 28 smaller exceptions. The four missing cases for (ν,8,7) BIBDs are provided, namely ν∈{48,56,96,448}, thereby establishing that the necessary existence conditions are sufficient without exception. Some constructions for resolvable designs are also provided, reducing the existence question for (ν,8,7) RBIBDs to 21 possible exceptions. © 2001 John Wiley & Sons, Inc. J Combin Designs 9: 334–356, 2001
A note on 3‐factorizations of K10*
Authors: Khosrovshahi G. B. , Maysoori Ch. , Tayfeh‐Rezaie B. 
Published: August 2002

AbstractIn this note, we enumerate all nonisomorphic 3‐factorizations of K10. © 2001 John Wiley & Sons, Inc. J Combin Designs 9: 379–383, 2001
Possible automatic cell classification of bone marrow aspirate using the CELL‐DYN 4000® automatic blood cell analyzer
Authors: Yamamura Ryousuke , Yamane Takahisa , Hino Masayuki , Ohta Kensuke , Shibata Hisako , Tsuda Izumi , Tatsumi Noriyuki 
Published: August 2002

AbstractIn clinical hematology, the demand for bone marrow aspiration testing is increasing. However, conventional automatic blood cell analyzers cannot completely analyze erythroblasts, and evaluation has mainly been performed by visual examination (the microscopic method). Using the CELL‐DYN 4000® automatic blood cell analyzer (CD4000) (Abbott Laboratories, North Chicago, IL), specific recognition and classification of erythroblasts by DNA staining is possible. In the present study, using bone marrow blood collected from normal subjects and patients with hematological malignancy, we classified cells by the microscopic method and with the CD4000, and compared the results. Good correlations were found for total nucleated cell count (TNCC), neutrophils, lymphocytes, erythroblasts, and the myeloid series to erythroid series (M/E) ratio. It is possible to detect blasts that emerge in patients with hematological malignancy using the blast flag system installed on the CD4000. Since all of the items can be analyzed in about 80 sec with the CD4000, cells in bone marrow aspirates can be classified faster with this apparatus than by the microscopic method. Therefore, analysis of bone marrow aspirates with this apparatus appears to be very useful not only for laboratory testing but also for clinical screening. J. Clin. Lab. Anal. 16:86–90, 2002. © 2002 Wiley‐Liss, Inc.
Commentary: Advances in the laboratory diagnosis of celiac disease
Authors: McPherson Richard A. 
Published: August 2002

AbstractAnti‐endomysial autoantibodies are very useful in the diagnosis and monitoring of celiac disease (gluten‐sensitive enteropathy) due to their high sensitivity and specificity for that disorder. The recent discovery that the autoantigen responsible for the endomysial pattern is tissue transglutaminase (tTG) has led to the commercial development of automated enzyme immunoassays for quantitation of that autoantibody. These assays are standardized to provide highly accurate and comparable testing between laboratories for anti‐tTG autoantibodies. Celiac disease is a common genetic disease in populations of Europe and the United States. It has a spectrum of expression ranging from silent or mild to severe, with resulting malabsorption that produces multiple‐organ system effects due to malnutrition. Many cases miss the diagnosis because the symptoms are not classic or the clinical syndrome is not severe. Because the treatment of celiac disease (avoidance of wheat products) is so effective and inexpensive and because celiac disease is so common in selective populations, a highly reliable test for its detection such as anti‐tTG should find wide application in clinical practice. J. Clin. Lab. Anal. 15:105–107, 2001. © 2001 Wiley‐Liss, Inc.
IgA antibodies against endomysium and transglutaminase: A comparison of methods
Authors: Jaskowski Troy D. , Schroder Carl , Martins Thomas B. , Litwin Christine M. , Hill Harry R. 
Published: August 2002

AbstractRecently, the endomysial antigen has been identified as the protein cross‐linking enzyme known as tissue transglutaminase (tTG). Our objective was to compare a novel enzyme immunoassay (EIA) that detects IgA antibody against tTG to two standard IFA methods utilizing thin tissue sections of rat kidney/rat stomach (KS) and distal primate esophagus (PE) as substrates to detect IgA antibody against endomysium (EMA). Sera from 100 patients suspected of having gluten‐sensitive enteropathy (GSE) and 23 sera possessing various antibodies used for EIA cross‐reactivity studies were included. Additional tests, performed routinely in our laboratory, were utilized to further assess sera from patients suspected having GSE. These tests include anti‐gliadin IgA antibody (AGA) and anti‐reticulin IgA antibody (ARA) and are part of the European Society for Pediatric Gastroenterology and Nutrition (ESPGAN) revised criteria for diagnosing GSE. When compared to IFA using KS, the tTG EIA had a sensitivity of 87.5%, was 97.1% specific, and had an overall agreement of 94.0%. When compared to IFA using PE, the tTG EIA had a sensitivity of 92.6%, was 93.2% specific, and had an overall agreement of 93.0%. When the KS IFA was compared to the PE IFA for EMA, the KS IFA had a sensitivity of 96.3%, was 91.8% specific, and had an overall agreement of 93.0%. The majority of sera that were positive for tTG but were negative by IFA (KS, n = 2/PE, n = 5) possessed IgA antibodies against gliadin and/or reticulin. Five of six sera with negative results by PE IFA were positive by the KS IFA and possessed one or more antibodies to tTG and/or gliadin and/or reticulin. We conclude that the tTG EIA compares well to both KS and PE IFAs when detecting IgA antibody against endomysium. We do not recommend the use of PE to detect EMA primarily because of the inconsistencies (i.e., tissue selection, quality, and preparation) and limited availability of commercially prepared PE tissue. J. Clin. Lab. Anal. 15:108–111, 2001. © 2001 Wiley‐Liss, Inc.
Differential distribution of soluble and complexed forms of prostate&hyphen;specific antigen in cyst fluids of women with gross cystic breast disease
Authors: Malatesta Manuela , Mannello Ferdinando , Sebastiani Maurizio , Gazzanelli Giancarlo 
Published: August 2002

AbstractGross cystic breast disease &lpar;GCBD&rpar; is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate&hyphen;specific antigen &lpar;PSA&rpar; molecular forms, and to correlate the different intracystic PSA profiles to the subpopulations of gross cysts. Type I cysts showed a median value of 0.71 &mgr;g&sol;L of total PSA and 0.32 g&sol;L of ACT, significantly different to that of Type II cysts &lpar;Wilcoxon P &lt; 0.001&rpar;. Although large excesses of ACT were detected in all samples, BCF samples and apocrine cells from Type I gross cysts contained about 70&percnt; of free PSA, compared to the higher amounts of complexed PSA found in Type II gross cysts. We demonstrate that in apocrine&sol;secretive Type I breast gross cysts the serine protease PSA was mainly present in its free form, in contrast to a major proportion of complexed PSA found in flattened&sol;transudative Type II cysts. Our results are consistent with the notion that a prolonged exposure of apocrine breast cells lining the Type I gross cysts to the proteolytic activity of PSA could be involved in the etiopathogenesis of GCBD. J. Clin. Lab. Anal. 15:81&ndash;86, 2001. &copy; 2001 Wiley&hyphen;Liss, Inc.
An examination of the psychometric structure of the Multidimensional Pain Inventory
Authors: Deisinger Julie A. , Cassisi Jeffrey E. , Lofland Kenneth R. , Cole Patricia , Bruehl Stephen 
Published: August 2002

AbstractFactor analyses to create the Multidimensional Pain Inventory (MPI) warrant further examination due to small sample size, the use of separate factor analyses for each MPI section, and lack of evidence for the replicability of the factor model. The present study randomly assigned 972 respondents to one of three samples. Study 1 used data from Sample 1 (n = 452) to examine the replicability of the MPI factor structure reported by Kerns, Turk, and Rudy (1985), and to measure the internal consistencies of MPI scales. In Study 2, confirmatory factor analyses using Sample 2 data (n = 267) verified a revised MPI factor structure that was generated in Study 1; cross‐validation was achieved using data from Sample 3 (n = 253). The revised MPI model differed from the original model in its number of scales and pattern of scale loadings. Recommendations for the refinement of the MPI are proposed. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 765–783, 2001.
A psychodynamic perspective on resistance in psychotherapy: Vive la résistance
Authors: Messer Stanley B. 
Published: August 2002

AbstractThe term resistance has an overly negative connotation, indicating a recalcitrant, oppositional tendency on the part of psychotherapy clients. This article emphasizes the inevitability and ubiquity of resistance and argues that it should be greeted as a therapist's friend, not as an enemy. It is the way in which clients present themselves to the world in general and to the therapist in particular. Five forms of resistance are presented, including: resistance to the recognition of feelings, fantasies, and motives; resistance to revealing feelings toward the therapist; resistance as a way of demonstrating self‐sufficiency; resistance as clients' reluctance to change their behavior outside the therapy room; and resistance as a function of failure of empathy on the part of the therapist. Vignettes from the author's practice and from the cases presented in this issue are discussed in terms of these five modes of resistance and their treatment. © 2002 John Wiley & Sons, Inc. J Clin Psychol/In Session 58: 157–163, 2002.
An interpersonal communication perspective on resistance in psychotherapy
Authors: Van Denburg Todd F. , Kiesler Donald J. 
Published: August 2002

AbstractThis article discusses resistance in psychotherapy using the contemporary interpersonal communication model of psychotherapy. This perspective defines resistance as moments during sessions when the patient and therapist are interacting with one another in such a way that the patient is kept from becoming aware of any covert experiences or transactional patterns that are conflictual and anxiety provoking. The ways in which resistance may be conceptualized and worked with are discussed and applied to three patient vignettes, with an emphasis on working with resistances as they are manifested in the patient–therapist relationship. Some of the potential reactions of the therapist to resistance are discussed, including some relatively beneficial and some problematic instances. © 2002 John Wiley & Sons, Inc. J Clin Psychol/In Session 58: 195–205, 2002.