bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of 17 new whiskered auklet (Aethia pygmaea) microsatellite loci in 42 seabirds identifies 5–15 polymorphic markers for each of nine Alcinae species
Authors: DAWSON DEBORAH A. , HUNTER FIONA M. , PANDHAL JAGROOP , BUCKLAND ROBERT , PARHAM ANDREW , JONES IAN L. , BRADSHAW MATTHEW , JEHLE ROBERT , BURKE TERRY 
Published: May 2005

AbstractWe isolated 17 microsatellite loci in the whiskered auklet (Aethia pygmaea) and tested them for amplification in 48 species from 13 seabird families (including 42 seabirds). Fifteen of these loci were also tested for polymorphism in 38 of the species, which included nine species of Alcinae (four auklets, Atlantic puffin, dovekie, razorbill and two murre species). On the average, nine loci were polymorphic per Alcinae species.
Twelve microsatellite markers developed in woundfin (Plagopterus argentissimus), an endangered warmwater fish of the lower Colorado River basin
Authors: VU NINH V. , KEELER‐FOSTER CONNIE L. , SPIES INGRID B. , RIBEIRO DANIEL T. 
Published: May 2005

AbstractWoundfin (Plagopterus argentissimus) is one of several endangered warmwater fishes once common to the lower Colorado River basin. Dexter National Fish Hatchery & Technology Center maintains a refugium stock founded in 1982, and augmented with c. 1000 young fish in 1989. Genetic characterization of the refugium stock and salvaged fish from the Virgin River is necessary to develop a strategy to incorporate salvaged fish into the hatchery stock. We developed an enriched microsatellite library and 12 species‐specific polymorphic loci. Alleles per locus ranged from 10 to 19, averaging 13. The average expected and observed heterozygosities were 0.884 and 0.895, respectively.
Highly polymorphic microsatellite loci in the bank vole (Clethrionomys glareolus)
Authors: BARKER F. S. , HELYAR S. J. , KEMP S. J. , BEGON M. 
Published: May 2005

AbstractWe describe seven polymorphic, dinucleotide microsatellite loci isolated from bank voles (Clethrionomys glareolus, Rodentia: Muridae) collected from the Wirral Peninsula, United Kingdom. Microsatellites were isolated as part of a long‐term study on the wider effects of host–pathogen interactions of an endemic viral disease. These microsatellites showed between five and 13 alleles per locus in these populations. Observed and expected heterozygosities varied between 0.275 to 0.777 and 0.487 to 0.794, respectively. These markers will allow us to investigate the structure of this bank vole population.
Isolation and characterization of tetramicrosatellite DNA markers in the Eurasian otter (Lutra lutra)
Authors: HUANG CHUAN‐CHIN , HSU YU‐CHENG , LEE LING‐LING , LI SHOU‐HSIEN 
Published: May 2005

AbstractEight new tetramicrosatellite loci for Eurasian otter (Lutra lutra) were designed. Polymorphism of these eight loci in 29 otter individuals was tested. The results indicated that the allele numbers of each of loci ranged from three to five and the observed heterozygosity from 0.483 to 0.828. These new loci can be useful for population genetic research on otters and help improve the resolution of individual identification using noninvasive method.
Cross‐species amplification of microsatellite loci in Aquilegia and Semiaquilegia (Ranunculaceae)
Authors: YANG JI Y. , COUNTERMAN BRIAN A. , ECKERT CHRISTOPHER G. , HODGES SCOTT A. 
Published: May 2005

AbstractWe developed 16 microsatellite loci from an F2 hybrid between Aquilegia formosa and Aquilegia pubescens. In samples of 28 individuals, we found an average of 14 alleles per locus from each parental species. We tested these loci for cross‐amplification in 10 additional species of Aquilegia and found that all 16 loci amplified in other North American species and 12 consistently amplified in European or Asian species. Nine loci amplified in the sister species to Aquilegia, Semiaquilegia adoxoides. The success of cross‐species amplification suggests that these microsatellites should prove useful for studies in a broad range of Aquilegia species.
Isolation and characterization of microsatellite markers from Musa balbisiana
Authors: BUHARIWALLA H. K. , JARRET R. L. , JAYASHREE B. , CROUCH J. H. , ORTIZ R. 
Published: May 2005

AbstractThis is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. ‘Tani’ (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between one and seven. All 25 microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB).
Isolation of seven polymorphic microsatellites in Ophioblennius atlanticus atlanticus (Perciformes, Blenniidae)
Authors: DE GIROLAMO M. , GRAPPUTO A. , ZANE L. , SANTOS R. S. , PALLAVICINI A. 
Published: May 2005

AbstractWe isolated and characterized seven polymorphic microsatellite loci of Ophioblennius atlanticus atlanticus (Valenciennes, 1836) using an optimized protocol to construct and screen a microsatellite‐enriched genomic library. The analysis of variability was performed in 16 specimens from Faial Island (Azores, Portugal). The mean number of alleles was 8.71 ± 2.43 and the level of expected heterozygosity ranged from 0.764 to 0.903. The total exclusionary probabilities using these loci for the first and the second parent were 0.985 and 0.998, respectively, suggesting that these microsatellites are a useful tool for large‐scale parentage analysis.
Microsatellites for tree of heaven (Ailanthus altissima)
Authors: DALLAS JOHN F. , LEITCH MALCOLM J. B. , HULME PHILIP E. 
Published: May 2005

AbstractNine (CT)n microsatellites were developed for tree of heaven, Ailanthus altissima, from invasive populations on the Mediterranean islands. These loci had seven to 12 alleles in 96 trees from five islands. Two loci had significant deficits of heterozygotes within islands while the other loci were in Hardy–Weinberg equilibrium, and four pairs of loci had significant linkage disequilibrium within a single island. These loci were also polymorphic in one to three individuals of the tree of heaven varieties, Ailanthus altissima erythrocarpa, Ailanthus altissima sutchuenensis and Ailanthus altissima tanakai, and the related species Ailanthus giraldii and Ailanthus vilmariniana.
Isolation and characterization of highly polymorphic microsatellites in the aquatic plant, Nymphoides peltata (Menyanthaceae)
Authors: UESUGI R. , TANI N. , GOKA K. , NISHIHIRO J. , TSUMURA Y. , WASHITANI I. 
Published: May 2005

AbstractTen microsatellite loci were described for conservation design of a threatened clonal aquatic plant, Nymphoides peltata. The microsatellite loci obtained through the construction of an enriched library were polymorphic (2–6 alleles per locus) and exhibited high levels of observed (0.333–0.889) and expected (0.284–0.765) heterozygosities. All microsatellite loci were expected to be useful for identification of genets and evaluation of genetic diversity of this species.
Development and characterization of microsatellite markers for two dioecious Ficus species
Authors: ZAVODNA MONIKA , ARENS PAUL , VAN DIJK PETER J. , VOSMAN BEN 
Published: May 2005

AbstractMicrosatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di‐, tri‐ and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross‐amplified in the other species, indicating transportability of the markers within the genus Ficus.
Polymorphic microsatellites for the study of Aconitum napellus L. (Ranunculaceae), a rare species in France
Authors: CADRE SOLENN LE , BOISSELIER‐DUBAYLE MARIE‐CATHERINE , LAMBOURDIÈRE JOSIE , MACHON NATHALIE , MORET JACQUES , SAMADI SARAH 
Published: May 2005

Abstract Aconitum napellus is a perennial European Ranunculaceae that is currently decreasing in France. Six polymorphic microsatellite markers were characterized to study the polymorphism among individuals sampled from six populations in France. The total number of alleles per locus varied from two to four. Mean observed heterozygosities at each microsatellite locus ranged from 0.05 to 0.19. This set of microsatellites offers an efficient tool for more detailed investigations on population genetic structure of a rare species that can reproduce both sexually and by vegetative spread.
Microsatellite markers for the whelk Buccinum undatum
Authors: WEETMAN DAVID , HAUSER LORENZ , SHAW PAUL W. , BAYES MICHELLE K. 
Published: May 2005

AbstractFive microsatellite loci are described for the commercially exploited marine gastropod, Buccinum undatum. Levels of polymorphism were variable with three to 19 alleles per locus and expected heterozygosities of 0.26–0.94 in 60 individuals of the population from which the loci were isolated. Homozygote excess at two of the loci might be attributable to null alleles, and these loci should not be used in, for example, parentage analysis. Nevertheless, because null allele frequencies can be estimated and their effects partitioned, all are useful markers for studies of population differentiation.
Isolation and characterization of eight microsatellite loci for the Neotropical freshwater catfish Pimelodella chagresi (Teleostei: Pimelodidae)
Authors: MOESER ANDREW A. , BERMINGHAM ELDREDGE 
Published: May 2005

AbstractWe report eight (CA)10−35 unlinked microsatellite loci from the Neotropical freshwater catfish, Pimelodella chagresi (Siluriformes: Pimelodidae). These loci were characterized with 23 individuals collected in Panama. Number of alleles per locus varied from 7 to 23 (mean = 12.9) and observed heterozygosity ranged from 0.522 to 0.909 (mean = 0.732). These loci will be used to investigate the existence of cryptic species within the P. chagresi clade, and to study fine‐scale population structure.
Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae)
Authors: OTERO‐ARNAIZ ADRIANA , SCHNABEL ANDREW , GLENN TRAVIS C. , SCHABLE NANCY A. , HAGEN CRIS , NDONG LEILA 
Published: May 2005

AbstractWe have developed primer pairs for 24 microsatellite loci isolated from Acacia brevispica, which is widespread in woodland savannas of East Africa. The loci were screened for levels of variation using 16–52 individuals from Mpala Research Centre, Kenya. Number of alleles per locus ranged from two to 17, and polymorphic information content ranged from 0.248 to 0.864. Several loci showed the presence null alleles, but many loci will be useful in ongoing studies of genetic structure, gene flow, breeding systems, and natural selection.
A panel of polymorphic microsatellites in the threatened Apennine chamois (Rupicapra pyrenaica ornata)
Authors: LORENZINI RITA 
Published: May 2005

AbstractThe Apennine chamois (Rupicapra pyrenaica ornata) is a highly endangered mountain ungulate. About 800 individuals still survive in central Apennines, Italy. Current conservation projects involve genetic identification of single animals, paternity tests, and assessment of relatedness. A suite of 60 microsatellite primer pairs from domestic bovids and cervids revealed polymorphisms for 10 loci, providing evidence for a low level of genetic variability, which might be a consequence of severe population bottlenecks and prolonged permanence at low numbers.
Isolation and characterization of six polymorphic microsatellite loci in Aspergillus niger
Authors: ESTEBAN ALEXANDRE , LEONG SU‐LIN L. , TRAN‐DINH NAI 
Published: May 2005

AbstractCertain strains of Aspergillus niger produce ochratoxin A in food and in animal feeds. Six polymorphic microsatellite markers suitable for population analysis were developed for A. niger through screening published sequences for microsatellite repeats. Polymorphism was evaluated for 28 isolates of A. niger, including toxigenic strains. Loci displayed six to 13 alleles. Investigation of cross‐species amplifications with Aspergillus carbonarius and Aspergillus japonicus showed limited success.
Isolation and characterization of microsatellite markers from the entomopathogenic fungus Metarhizium anisopliae
Authors: ENKERLI JÜRG , KÖLLIKER ROLAND , KELLER SIEGFRIED , WIDMER FRANCO 
Published: May 2005

AbstractFourteen polymorphic microsatellite markers were isolated from the entomopathogenic fungus, Metarhizium anisopliae, based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 34 isolates sampled from across Switzerland. The number of detected alleles in 14 loci ranged from two to eight and expected heterozygosity from 0.265 to 0.808. Because of the high expected heterozygosity, the 14 microsatellite loci are very useful for ecological studies and analysis of population diversity, and to identifying, monitoring, and tracking M. anisopliae strains applied as biological control agents.
Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)
Authors: FAIRCLOTH BRANT C. , REID ALLISON , VALENTINE TERESA , EO SOO HYUNG , TERHUNE THERON M. , GLENN TRAVIS C. , PALMER WILLIAM E. , NAIRN CAMPBELL JOSEPH , CARROLL JOHN P. 
Published: May 2005

AbstractWe describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).
Microsatellites from the burbot (Lota lota), a freshwater gadoid fish (Teleostei)
Authors: SANETRA MATTHIAS , MEYER AXEL 
Published: May 2005

AbstractWe developed 21 polymorphic dinucleotide microsatellite loci, (CA)n and (CT)n, for the Holarctic freshwater fish, Lota lota, using an enriched genomic library protocol. The species has an interesting life history because winter‐spawning adults migrate over long distances to form spawning aggregations, a behaviour which should maintain genetic homogeneity across large spatial scales. Availability of the reported microsatellites will facilitate the investigation of population genetic structure with regard to postglacial colonization history and conservation strategies. The primers were screened on 30 individuals from a natural population (Lake Constance, southern Germany), revealing three to 24 alleles per locus with expected heterozygosities ranging from 0.48 to 0.93.
Novel polymorphic nuclear microsatellites in Cupressus sempervirens L.
Authors: SEBASTIANI F. , BUONAMICI A. , FINESCHI S. , RACCHI M. L. , RADDI P. , VENDRAMIN G. G. 
Published: May 2005

AbstractWe report the characterization and optimization of nine polymorphic nuclear microsatellites from Cupressus sempervirens L. using an enriched library method. A total of 24 individuals from four different populations were used to estimate genetic diversity parameters. High level of allelic diversity was found with a number of alleles ranging from two to 13. These markers will prove very useful in screening diversity at different geographical scales and in monitoring gene flow in cypress orchards.
Isolation and characterization of microsatellite loci from the Australian endemic mountain pygmy‐possum, Burramys parvus Broom
Authors: MITROVSKI P. , HEINZE D. A. , GUTHRIDGE K. , WEEKS A. R. 
Published: May 2005

AbstractThe endemic mountain pygmy‐possum, Burramys parvus, is an endangered Australian marsupial restricted to mainland alpine regions. Population structure, breeding system and gene flow are crucial for the development of effective conservation strategies, but have not been investigated in B. parvus. Here we have isolated eight polymorphic microsatellite markers from B. parvus to investigate these parameters. We found two to 12 alleles with observed heterozygosity values ranging from 0.321 to 0.878 for these loci in initial estimates from a single population.
Development and diversity of microsatellite markers for endangered species, Morus boninensis Koidz., to establish conservation program
Authors: TANI NAOKI , KAWAHARA TAKAYUKI , YOSHIMARU HIROSHI 
Published: May 2005

AbstractWe have developed seven microsatellite markers from an enrichment library of genomic DNA for an endangered species, Morus boninensis. A total of 112 of the 320 clones were found to have unique sequences with microsatellite repeats. Seven of 54 primer pairs revealed clear chromatograms and polymorphisms among 36 individuals sampled from three of the Bonin Islands. Seven to 17 alleles per locus were detected, and the expected heterozygosity without considering double reduction ranged from 0.429 to 0.819. These findings should be useful for those studying the conservation genetics of M. boninensis.
Characterization of 12 single nucleotide polymorphisms in weathervane scallop
Authors: ELFSTROM C. M. , GAFFNEY P. M. , SMITH C. T. , SEEB J. E. 
Published: May 2005

AbstractWe describe 27 single nucleotide polymorphisms (SNPs) in a commercially important bivalve, the weathervane scallop (Patinopecten caurinus), identified using a targeted‐gene approach. We further characterize 12 of these using 5′‐nuclease and allele‐specific PCR assays. Polymorphisms were identified in both mitochondrial and nuclear genes. These are the first SNPs developed for delineating population structure in the weathervane scallop and will provide a useful complement to currently available genetic markers.
Isolation and characterization of microsatellite loci in the dark bush cricket, Pholidoptera griseoaptera (Tettigoniidae)
Authors: ARENS P. , WERNKE‐LENTING J. H. , DIEKÖTTER T. , ROTHENBÜHLER C. , SPEELMANS M. , HENDRICKX F. , SMULDERS M. J. M. 
Published: May 2005

AbstractTwelve novel polymorphic microsatellite loci are presented for the dark bush cricket, Pholidoptera griseoaptera. All loci are polymorphic, with up to 37 alleles per locus. These microsatellites will be useful tools for studying the influence of landscape structure and land use intensity in agricultural landscapes on genetic diversity within and among populations of P. griseoaptera.
Multiplex PCR system applied for analysing microsatellite loci of Schlegel's black rockfish, Sebastes schlegeli
Authors: YOSHIDA K. , NAKAGAWA M. , WADA S. 
Published: May 2005

AbstractTwo multiplex PCR amplifications were performed to analyse six microsatellite loci of Schlegel's black rockfish, Sebastes schlegeli, an important commercial fish in the northern part of Japan and an important species for the stock enhancement program in this area. We analysed 67 wild samples from Yamada Bay, Iwate Prefecture, Japan. The observed genotype frequencies agreed with the Hardy–Weinberg expectations at all loci, and the observed heterozygosities ranged from 0.072 to 0.897.
Isolation and characterization of microsatellite loci in the Alpine leaf beetle, Oreina elongata
Authors: MARGRAF N. , GAUTSCHI B. , RAHIER M. 
Published: May 2005

AbstractFor a study of local adaptations in the Alpine leaf beetle, Oreina elongata, we developed six microsatellite loci and screened them in 305 individuals from 13 populations. All markers were polymorphic with three to 15 alleles per locus. Average observed and expected heterozygosity values were 0.14 and 0.62, respectively. Four markers showed heterozygote deficiency and deviated significantly from Hardy–Weinberg expectations, indicating the presence of null alleles.
Isolation and characterization of 15 microsatellite loci in the Korean goral (Nemorhaedus caudatus)
Authors: AN JUNGHWA , MIN MI‐SOOK , SOMMER JULIE , LOUIS EDWARD , BRENNEMAN RICK , KWON SOO‐WHAN , SHIN NAM SHIK , LEE HANG 
Published: May 2005

AbstractMicrosatellites have been applied in a variety of fields, including conservation genetics. Species‐specific microsatellites are considered as more powerful genetic markers to generate an accurate genetic composition of a species itself. Accordingly, we characterized 15 polymorphic microsatellite loci from Korean goral,Nemorhaedus caudatus, one of the most endangered species in South Korea. The new markers should benefit future studies of the endangered species of other Asian gorals and their relatives for the study of genetic diversity and potential conservation management.
Characterization and isolation of DNA microsatellite primers in Raja clavata L. (thornback ray, Rajidae)
Authors: CHEVOLOT MALIA , REUSCH THORSTEN B. H. , BOELE‐BOS STELLA , STAM WYTZE T. , OLSEN JEANINE L. 
Published: May 2005

AbstractThe thornback ray, Raja clavata, is an elasmobranch (cartilaginous fish). Since the 1950s, its stock has severely declined. In order to investigate the genetic population structure of the species, we developed microsatellite loci. The five loci reported here have eight to 48 alleles per locus and display an observed heterozygosity ranging from 0.32 to 0.98 with no deviation from the Hardy–Weinberg equilibrium. In the test panel of 122 individuals from three populations, no null alleles, band‐stuttering, large allele dropouts or linkage disequilibrium was detected.
Isolation and characterization of microsatellite loci in the bee Ceratina flavipes
Authors: AZUMA N. , TAKAHASHI J. , KIDOKORO M. , HIGASHI S. 
Published: May 2005

AbstractPolymorphic microsatellite markers can provide essential information on genetic analyses of sociality and mating behaviour of insects. We developed eight microsatellite loci in the small carpenter bee, Ceratina flavipes, using a magnetic bead hybridization enrichment protocol. These loci showed two to eight alleles with expected heterozygosity of 0.21–0.87, and also seemed useful to such congeneric species as Ceratina okinawana distributed in southern Japan. By using these loci, the sociality and mating systems in Ceratina species, that are still controversial, are possible to be analysed.
Development and characterization of polymorphic microsatellite DNA loci for the endangered dusky gopher frog, Rana sevosa, and two closely related species, Rana capito and Rana areolata
Authors: RICHTER STEPHEN C. , BROUGHTON RICHARD E. 
Published: May 2005

AbstractA microsatellite library was developed using genomic DNA of the endangered dusky gopher frog, Rana sevosa. Polymerase chain reaction (PCR) primers and conditions are presented for R. sevosa (eight loci) and two sister taxa — other gopher frogs, Rana capito (seven loci) and crawfish frogs, Rana areolata (three loci). Polymorphism of each microsatellite locus was evaluated for each species. All loci have moderate to high genetic variation in terms of allelic richness (four to 10 alleles per locus), observed heterozygosity (0.595–0.946), and expected heterozygosity (0.531–0.856).
Isolation and characterization of eight microsatellite loci from the house finch (Carpodacus mexicanus)
Authors: HAWLEY DANA M. 
Published: May 2005

AbstractI isolated the first set of polymorphic microsatellite markers from the house finch, Carpodacus mexicanus, a well‐studied North American bird species, as part of an effort to compare levels of genetic diversity in introduced and native populations. Here, I describe eight independently assorting microsatellite loci screened for polymorphism using 40 house finches. Polymorphism levels ranged from six to 14 alleles (mean = 10.6), making these markers a powerful tool for paternity and population level analyses of this widely distributed North American species.