bluuebunny/crossref_metadata_2025_split huggingface dataset:
Germline Bottlenecks, Biparental Inheritance and Selection on Mitochondrial Variants
Authors: Roze Denis , Rousset François , Michalakis Yannis 
Published: May 2005

Abstract
Selection on mitochondrial mutations potentially occurs at different levels: at the mitochondria, cell, and organism levels. Several factors affect the strength of selection at these different levels; in particular, mitochondrial bottlenecks during germline development and reduced paternal transmission decrease the genetic variance within cells, while they increase the variance between cells and between organisms, thus decreasing the strength of selection within cells and increasing the strength of selection between cells and organisms. However, bottlenecks and paternal transmission also affect the effective mitochondrial population size, thus affecting genetic drift. In this article, we use a simple model of a unicellular life cycle to investigate the effects of bottlenecks and paternal transmission on the probability of fixation of mitochondrial mutants and their frequency at mutation-selection equilibrium. We find that bottlenecks and reduced paternal transmission decrease the mean frequency of alleles with sm &gt; sc (approximately), where sm and sc are the strengths of selection for an allele within and between cells, respectively, and increase the frequency of alleles with sm &lt; sc. Effects on fixation probabilities are different; for example, bottlenecks reduce the fixation probability of mutants with sm &gt; 0 (unless sm is very small relative to sc) and increase the fixation probability of mutants with sm &lt; 0.
Evolutionary Capacitance May Be Favored by Natural Selection
Authors: Masel Joanna 
Published: May 2005

Abstract
Evolutionary capacitors phenotypically reveal a stock of cryptic genetic variation in a reversible fashion. The sudden and reversible revelation of a range of variation is fundamentally different from the gradual introduction of variation by mutation. Here I study the invasion dynamics of modifiers of revelation. A modifier with the optimal rate of revelation mopt has a higher probability of invading any other population than of being counterinvaded. mopt varies with the population size N and the rate θ at which environmental change makes revelation adaptive. For small populations less than a minimum cutoff Nmin, all revelation is selected against. Nmin is typically quite small and increases only weakly, with θ−1/2. For large populations with N &gt; 1/θ, mopt is ∼1/N. Selection for the optimum is highly effective and increases in effectiveness with larger N ≫ 1/θ. For intermediate values of N, mopt is typically a little less than θ and is only weakly favored over less frequent revelation. The model is analogous to a two-locus model for the evolution of a mutator allele. It is a fully stochastic model and so is able to show that selection for revelation can be strong enough to overcome random drift.
The Course of Malaria in Mice: Major Histocompatibility Complex (MHC) Effects, but No General MHC Heterozygote Advantage in Single-Strain Infections
Authors: Wedekind Claus , Walker Mirjam , Little Tom J 
Published: May 2005

AbstractA general MHC-heterozygote advantage in parasite-infected organisms is often assumed, although there is little experimental evidence for this. We tested the response of MHC-congenic mice (F2 segregants) to malaria and found the course of infection to be significantly influenced by MHC haplotype, parasite strain, and host gender. However, the MHC heterozygotes did worse than expected from the average response of the homozygotes.
EGO-1, a Putative RNA-Directed RNA Polymerase, Promotes Germline Proliferation in Parallel With GLP-1/Notch Signaling and Regulates the Spatial Organization of Nuclear Pore Complexes and Germline P Granules in Caenorhabditis elegans
Authors: Vought Valarie E , Ohmachi Mitsue , Lee Min-Ho , Maine Eleanor M 
Published: May 2005

Abstract
Caenorhabditis elegans EGO-1, a putative cellular RNA-directed RNA polymerase, promotes several aspects of germline development, including proliferation, meiosis, and gametogenesis, and ensures a robust response to RNA interference. In C. elegans, GLP-1/Notch signaling from the somatic gonad maintains a population of proliferating germ cells, while entry of germ cells into meiosis is triggered by the GLD-1 and GLD-2 pathways. GLP-1 signaling prevents germ cells from entering meiosis by inhibiting GLD-1 and GLD-2 activity. We originally identified the ego-1 gene on the basis of a genetic interaction with glp-1. Here, we investigate the role of ego-1 in germline proliferation. Our data indicate that EGO-1 does not positively regulate GLP-1 protein levels or GLP-1 signaling activity. Moreover, GLP-1 signaling does not positively regulate EGO-1 activity. EGO-1 does not inhibit expression of GLD-1 protein in the distal germline. Instead, EGO-1 acts in parallel with GLP-1 signaling to influence the proliferation vs. meiosis fate choice. Moreover, EGO-1 and GLD-1 act in parallel to ensure germline health. Finally, the size and distribution of nuclear pore complexes and perinuclear P granules are altered in the absence of EGO-1, effects that disrupt germ cell biology per se and probably limit germline growth.
Repeat-Induced Point Mutation and the Population Structure of Transposable Elements in Microbotryum violaceum
Authors: Hood Michael E , Katawczik Melanie , Giraud Tatiana 
Published: May 2005

Abstract
Repeat-induced point mutation (RIP) is a genome defense in fungi that hypermutates repetitive DNA and is suggested to limit the accumulation of transposable elements. The genome of Microbotryum violaceum has a high density of transposable elements compared to other fungi, but there is also evidence of RIP activity. This is the first report of RIP in a basidiomycete and was obtained by sequencing multiple copies of the integrase gene of a copia-type transposable element and the helicase gene of a Helitron-type element. In M. violaceum, the targets for RIP mutations are the cytosine residues of TCG trinucleotide combinations. Although RIP is a linkage-dependent process that tends to increase the variation among repetitive sequences, a chromosome-specific substructuring was observed in the transposable element population. The observed chromosome-specific patterns are not consistent with RIP, but rather suggest an effect of gene conversion, which is also a linkage-dependent process but results in a homogenization of repeated sequences. Particular sequences were found more widely distributed within the genome than expected by chance and may reflect the recently active variants. Therefore, sequence variation of transposable elements in M. violaceum appears to be driven by selection for transposition ability in combination with the context-specific forces of the RIP and gene conversion.
A New Reversion Assay for Measuring All Possible Base Pair Substitutions in Saccharomyces cerevisiae
Authors: Williams Teresa-Marie , Fabbri Rebecca M , Reeves Jason W , Crouse Gray F 
Published: May 2005

Abstract
A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.
Hardy-Weinberg Testing of a Single Homozygous Genotype
Authors: Chen John J , Duan Tao , Single Richard , Mather Kristie , Thomson Glenys 
Published: May 2005

Abstract
No proper statistical test is available for the evaluation of deviation of a single homozygous genotype from Hardy-Weinberg equilibrium (HWE) proportion. We propose a 1-d.f. χ2-test. The power of the proposed test is favorable compared to existing HWE testing procedures. The applications of this test are discussed.
Genome-Wide Screen for Inner Nuclear Membrane Protein Targeting in Saccharomyces cerevisiae
Authors: Murthi Athulaprabha , Hopper Anita K 
Published: May 2005

Abstract
Appropriate nuclear membrane structure is important for all eukaryotic organisms as evidenced by the numerous human diseases and alterations in gene expression caused by inappropriate targeting of proteins to the inner nuclear membrane (INM). We report here the first genome-wide screen to identify proteins functioning in INM targeting. We transformed to near completion the 4850 members of the Saccharomyces cerevisiae deletion collection of unessential genes in the 96-well format with a plasmid encoding a reporter protein, Trm1-II-GFP, which normally resides at the INM. We found that deletion of genes encoding subunits of the N-terminal acetyltransferase, NatC, cause mislocation of Trm1-II-GFP from the INM to the nucleoplasm. Mass spectroscopic analysis indicates that Trm1-II-GFP is N-acetylated. N-terminal mutations of Trm1-II-GFP predicted to ablate N-acetylation cause nucleoplasmic location, whereas a variant with an N-terminal alteration predicted to allow N-acetylation by NatC is located at the INM, providing genetic support that Trm1p-II N-acetylation is necessary for its subnuclear INM location. However, because N-acetylation appears not to be sufficient for INM targeting, it may provide a necessary role for INM targeting by affecting Trm1-II-GFP structure and exposure of cis-acting INM targeting motifs. We also discovered that YIL090W/Ice2p, an integral membrane protein located in the endoplasmic reticulum, is necessary for efficient targeting of Trm1-II-GFP to the INM. YIL090W/Ice2p may serve as a tether for INM proteins or as a regulator of INM tethers. Our methodology can be extrapolated to obtain genome-wide perspectives of mechanisms necessary to achieve appropriate subcellular and/or suborganellar location for any resident protein.
A Major State Tobacco Tax Increase, the Master Settlement Agreement, and Cigarette Consumption: The California Experience
Authors: Sung Hai-Yen , Hu Teh-wei , Ong Michael , Keeler Theodore E. , Sheu Mei-ling 
Published: May 2005

Objectives. We evaluated the combined effects on California cigarette consumption of an additional 50¢ per pack state tax imposed by Proposition 10 of January 1999 and a 45¢ per pack increase in cigarette prices stemming from the Master Settlement Agreement (MSA) of November 1998. Methods. We used quarterly cigarette sales data for the period 1984–2002 to estimate a time-series intervention model adjusting for seasonal variations and time trend. Results. Over the period 1999 through 2002, the combined effect was to reduce cigarette consumption by 2.4 packs per capita per quarter (1.3 billion packs total over the 4-year period) and to raise state tax revenues by $2.1 billion. These effects were similar to the effects of a 25¢ per pack tax increase enacted by Proposition 99 a decade earlier, although with decreased relative effectiveness as measured by percentage of reduction in cigarette consumption divided by percentage of increase in taxation (−0.44 vs −0.60). Conclusions. A major increase in price through taxation and the MSA provided a strong economic disincentive for smokers in a state with a low smoking prevalence. This effect could be reinforced if part of the MSA payments were devoted to tobacco control programs.
Smoking in 6 Diverse Chicago Communities—A Population Study
Authors: Dell Jade L. , Whitman Steven , Shah Ami M. , Silva Abigail , Ansell David 
Published: May 2005

Objectives. We analyzed smoking survey data across communities in Chicago, Ill, to explore community-level variations in smoking behavior. Methods. We conducted a health survey of 6 racially and ethnically diverse Chicago communities during 2002–2003. The survey included questions about current smoking, smoking history, and cessation attempts. Results. Smoking prevalence varied from 18% in the wealthiest (predominately White) community to 39% in the poorest (predominately Black) community. In a contiguous pair of communities, one Mexican and the other Black, smoking prevalence varied by a factor of 2. Men, residents in poorer households and households without telephones, and residents with less education were most likely to smoke. Conclusions. The high proportions of current smokers who had attempted to quit indicate a prevalent desire to stop smoking. However, less than 4% of the Master Tobacco Settlement Agreement funds are being spent on smoking prevention, or even on health in general, in Illinois. Although much is known about the prevalence of smoking at the national level, few studies of smoking have been done at the community level, presenting difficulties for the allocation of resources and the design of smoking cessation programs in response to community needs. Understanding community-level smoking rates could improve the allocation of resources and assist the shaping of culturally meaningful prevention efforts.
Adult Tobacco Use Levels After Intensive Tobacco Control Measures: New York City, 2002–2003
Authors: Frieden Thomas R. , Mostashari Farzad , Kerker Bonnie D. , Miller Nancy , Hajat Anjum , Frankel Martin 
Published: May 2005

Objectives. We sought to determine the impact of comprehensive tobacco control measures in New York City. Methods. In 2002, New York City implemented a tobacco control strategy of (1) increased cigarette excise taxes; (2) legal action that made virtually all work-places, including bars and restaurants, smoke free; (3) increased cessation services, including a large-scale free nicotine-patch program; (4) education; and (5) evaluation. The health department also began annual surveys on a broad array of health measures, including smoking. Results. From 2002 to 2003, smoking prevalence among New York City adults decreased by 11% (from 21.6% to 19.2%, approximately 140000 fewer smokers). Smoking declined among all age groups, race/ethnicities, and education levels; in both genders; among both US-born and foreign-born persons; and in all 5 boroughs. Increased taxation appeared to account for the largest proportion of the decrease; however, between 2002 and 2003 the proportion of cigarettes purchased outside New York City doubled, reducing the effective price increase by a third. Conclusions. Concerted local action can sharply reduce smoking prevalence. However, further progress will require national action, particularly to increase cigarette taxes, reduce cigarette tax evasion, expand education and cessation services, and limit tobacco marketing.
Lifetime Exposure to Depression and Neuroimaging Measures of Brain Structure and Function
Authors: Wang Xinyi , Hoffstaedter Felix , Kasper Jan , Eickhoff Simon B. , Patil Kaustubh R. , Dukart Juergen 
Published: February 2024

ImportanceDespite decades of neuroimaging studies reporting brain structural and functional alterations in depression, discrepancies in findings across studies and limited convergence across meta-analyses have raised questions about the consistency and robustness of the observed brain phenotypes.ObjectiveTo investigate the associations between 6 operational criteria of lifetime exposure to depression and functional and structural neuroimaging measures.Design, Setting, and ParticipantsThis cross-sectional study analyzed data from a UK Biobank cohort of individuals aged 45 to 80 years who were enrolled between January 1, 2014, and December 31, 2018. Participants included individuals with a lifetime exposure to depression and matched healthy controls without indications of psychosis, mental illness, behavior disorder, and disease of the nervous system. Six operational criteria of lifetime exposure to depression were evaluated: help seeking for depression; self-reported depression; antidepressant use; depression definition by Smith et al; hospital International Statistical Classification of Diseases and Related Health Problems, Tenth Revision (ICD-10) diagnosis codes F32 and F33; and Composite International Diagnostic Interview Short Form score. Six increasingly restrictive depression definitions and groups were defined based on the 6 depression criteria, ranging from meeting only 1 criterion to meeting all 6 criteria. Data were analyzed between January and October 2022.Main Outcomes and MeasuresFunctional measures were calculated using voxel-wise fractional amplitude of low-frequency fluctuation (fALFF), global correlation (GCOR), and local correlation (LCOR). Structural measures were calculated using gray matter volume (GMV).ResultsThe study included 20 484 individuals with lifetime depression (12 645 females [61.7%]; mean [SD] age, 63.91 [7.60] years) and 25 462 healthy controls (14 078 males [55.3%]; mean [SD] age, 65.05 [7.8] years). Across all depression criteria, individuals with lifetime depression displayed regionally consistent decreases in fALFF, LCOR, and GCOR (Cohen d range, −0.53 [95% CI, −0.88 to −0.15] to −0.04 [95% CI, −0.07 to −0.01]) but not in GMV (Cohen d range, −0.47 [95 % CI, −0.75 to −0.12] to 0.26 [95% CI, 0.15-0.37]). Hospital ICD-10 diagnosis codes F32 and F33 (median [IQR] difference in effect sizes, −0.14 [−0.17 to −0.11]) and antidepressant use (median [IQR] difference in effect sizes, −0.12 [−0.16 to −0.10]) were criteria associated with the most pronounced alterations.Conclusions and RelevanceResults of this cross-sectional study indicate that lifetime exposure to depression was associated with robust functional changes, with a more restrictive depression definition revealing more pronounced alterations. Different inclusion criteria for depression may be associated with the substantial variation in imaging findings reported in the literature.
Realizing High‐Flux Lithium‐Ion Conduction by LaF3 Doping in Quasi‐Solid‐State Electrolytes
Authors: Bi Linnan , He Liang , Song Yaochen , Wang Yi , Xie Qingyu , Dong Peng , Zhang Yingjie , Yao Yao , Liao Jiaxuan , Wang Sizhe 
Published: February 2024

AbstractThe inherent low ionic conductivity of PVDF‐based electrolytes at room temperature and lithium dendrite penetration hinder its further application. Herein, a LaF3 doped Poly(vinylidene fluoride‐chlorotrifluoroethylene) (P(VDF‐ctfe)) quasi‐solid electrolyte is developed. High‐flux channels are created due to the optimization of the lithium environment that lanthanum preferentially competes coordination with anionic species for relaxing lithium hopping. Lithium carriers are therefore highly free and unbound at the molecular level, resulting in a high ionic conductivity (σ) of 0.7 mS cm−1 and a transfer number (tLi+) of 0.79 at 25 °C. Moreover, the in situ organic–inorganic LiF‐rich dielectric layer effectively improves the stability and compatibility of the electrode/electrolyte interface, ensuring interfacial lithium conduction while facilitating stable Li+ plating/stripping. As a result, the optimized Li/ATCSE‐3%/Li can deliver favorable compatibility at 0.1 and 0.3 mA cm−2 for stable Li+ plating/stripping for 2000 and 1200 h, respectively. The high‐mass loading (6.4 mg cm−2) pouch cell delivers a stable cycling performance over 100 cycles with a capacity retention of 85.8% at 0.3 °C. This work is anticipated to provide considerable insight into the creative design of lithium transport of polymer‐based for practical quasi‐solid‐state lithium metal batteries.
Monolithic Fabrication of Metal‐Free On‐Paper Self‐Charging Power Systems
Authors: Su Yingchun , Xue Han , Fu Yujie , Chen Shiqian , Li Zheng , Li Lengwan , Knoks Ainars , Bogdanova Olga , Lesničenoks Pēteris , Palmbahs Roberts , Laurila Mika‐Matti , Mäntysalo Matti , Hammar Mattias , Hallén Anders , Nordell Nils , Li Jiantong 
Published: February 2024

AbstractSelf‐charging power systems (SCPSs) are envisioned as promising solutions for emerging electronics to mitigate the increasing global concern about battery waste. However, present SCPSs suffer from large form factors, unscalable fabrication, and material complexity. Herein, a type of highly stable, eco‐friendly conductive inks based on poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) are developed for direct ink writing of multiple components in the SCPSs, including electrodes for miniaturized spacer‐free triboelectric nanogenerators (TENGs) and microsupercapacitors (MSCs), and interconnects. The principle of “one ink, multiple functions” enables to almost fully print the entire SCPSs on the same paper substrate in a monolithic manner without post‐integration. The monolithic fabrication significantly improves the upscaling capability for manufacturing and reduces the form factor of the entire SCPSs (a small footprint area of ≈2 cm × 3 cm and thickness of ≈1 mm). After pressing/releasing the TENGs for ≈79000 cycles, the 3‐cell series‐connected MSC array can be charged to 1.6 V while the 6‐cell array to 3.0 V. On‐paper SCPSs are promising to serve as lightweight, thin, sustainable, and low‐cost power supplies.
Surface Reconditioning of Lithium Metal Electrodes by Laser Treatment for the Industrial Production of Enhanced Lithium Metal Batteries
Authors: Kriegler Johannes , Ballmes Heiko , Dib Serge , Demir Ali Gökhan , Hille Lucas , Liang Yunhao , Wach Lovis , Weinmann Steffen , Keilhofer Josef , Kim Kun Joong , Rupp Jennifer L. M. , Zaeh Michael F. 
Published: February 2024

AbstractIncorporating lithium metal anodes in next‐generation batteries promises enhanced energy densities. However, lithium's reactivity results in the formation of a native surface film, affecting battery performance. Therefore, precisely controlling the chemical and morphological surface condition of lithium metal anodes is imperative for producing high‐performance lithium metal batteries. This study demonstrates the efficacy of laser treatment for removing superficial contaminants from lithium metal substrates. To this end, picosecond‐pulsed laser radiation is proposed for modifying the surface of lithium metal substrates. Scanning electron microscopy (SEM) revealed that different laser process regimes can be exploited to achieve a wide spectrum of surface morphologies. Energy‐dispersive X‐ray spectroscopy (EDX) confirmed substantial reductions of ≈80% in oxidic and carbonaceous surface species. The contamination layer removal translated into interfacial resistance reductions of 35% and 44% when testing laser‐cleaned lithium metal anodes in symmetric all‐solid‐state batteries (ASSBs) with lithium phosphorus sulfur chloride (LPSCl) and lithium lanthanum zirconium oxide (LLZO) solid electrolytes, respectively. Finally, a framework for integrating laser cleaning into industrial battery production is suggested, evidencing the industrial feasibility of the approach. In summary, this work advances the understanding of lithium metal surface treatments and serves as proof of principle for its industrial applicability.
Bi/BSO Heterojunctions via Vacancy Engineering for Efficient Photocatalytic Nitrogen Fixation
Authors: Wu Runjie , Gao Shuai , Jones Colton , Sun Mingming , Guo Ming , Tai Ran , Chen Shaowei , Wang Qiang 
Published: February 2024

AbstractPhotocatalytic nitrogen reduction represents a viable technology for green ammonia synthesis under mild conditions. However, the performance of the photocatalysts is typically limited by high charge carrier recombination and low adsorption and activation of nitrogen molecules. Herein, Bi/Bi2Sn2O7 (Bi/BSO) heterojunction nanocomposites are prepared via a one‐step hydrothermal method, where NaOH etching of oxygen vacancies in the Bi─O bonds of Bi2Sn2O7 (BSO) is exploited for the in situ formation of metallic Bi and hence Schottky junctions with the semiconducting BSO. This leads to a high separation rate of photogenerated charge carriers. Consequently, compared to the pure‐phase BSO, the Bi/BSO heterostructures exhibit markedly enhanced ammonia production, reaching an optimum rate of 284.5 µmol g−1 h−1, where the rectifying contact between the semiconducting BSO and metallic Bi facilitates directional BSO to Bi electron transfer, leading to enrichment of photogenerated electrons at the active sites of metallic Bi. First‐principles calculations confirm the alteration of active sites and the guided electron flow by the Schottky junctions and surface oxygen vacancies. Results from this study offer an effective paradigm of structural engineering in manipulating the photocatalytic activity of bismuth‐based pyrochlore materials toward nitrogen fixation to ammonia.