bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the Prognosis and Tumor Microenvironment of Cellular
Senescence-related Genes through scRNA-seq and Bulk RNA-seq Analysis
in GC
Authors: Guo Guoxiang , Zhou Zhifeng , Chen Shuping , Cheng Jiaqing , Wang Yang , Lan Tianshu , Ye Yunbin 
Published: October 2023

Background:
Cellular senescence (CS) is thought to be the primary cause of cancer
development and progression. This study aimed to investigate the prognostic role and molecular
subtypes of CS-associated genes in gastric cancer (GC).

Materials and Methods:
The CellAge database was utilized to acquire CS-related genes. Expression
data and clinical information of GC patients were obtained from The Cancer Genome
Atlas (TCGA) database. Patients were then grouped into distinct subtypes using the “Consesus-
ClusterPlus” R package based on CS-related genes. An in-depth analysis was conducted to assess
the gene expression, molecular function, prognosis, gene mutation, immune infiltration,
and drug resistance of each subtype. In addition, a CS-associated risk model was developed
based on Cox regression analysis. The nomogram, constructed on the basis of the risk score and
clinical factors, was formulated to improve the clinical application of GC patients. Finally, several
candidate drugs were screened based on the Cancer Therapeutics Response Portal (CTRP)
and PRISM Repurposing dataset.

Results:
According to the cluster result, patients were categorized into two molecular subtypes
(C1 and C2). The two subtypes revealed distinct expression levels, overall survival (OS) and
clinical presentations, mutation profiles, tumor microenvironment (TME), and drug resistance.
A risk model was developed by selecting eight genes from the differential expression genes
(DEGs) between two molecular subtypes. Patients with GC were categorized into two risk
groups, with the high-risk group exhibiting a poor prognosis, a higher TME level, and increased
expression of immune checkpoints. Function enrichment results suggested that genes were enriched
in DNA repaired pathway in the low-risk group. Moreover, the Tumor Immune Dysfunction
and Exclusion (TIDE) analysis indicated that immunotherapy is likely to be more beneficial
for patients in the low-risk group. Drug analysis results revealed that several drugs, including
ML210, ML162, dasatinib, idronoxil, and temsirolimus, may contribute to the treatment of GC
patients in the high-risk group. Moreover, the risk model genes presented a distinct expression
in single-cell levels in the GSE150290 dataset.

Conclusion:
The two molecular subtypes, with their own individual OS rate, expression patterns,
and immune infiltration, lay the foundation for further exploration into the GC molecular
mechanism. The eight gene signatures could effectively predict the GC prognosis and can serve
as reliable markers for GC patients.
MSSD: An Efficient Method for Constructing Accurate and Stable Phylogenetic Networks by Merging Subtrees of Equal Depth
Authors: Xing Jiajie , Song Xu , Yu Meiju , Wang Juan , Yu Jing 
Published: October 2023

Background:
Systematic phylogenetic networks are essential for studying the evolutionary
relationships and diversity among species. These networks are particularly important for capturing
non-tree-like processes resulting from reticulate evolutionary events. However, existing methods
for constructing phylogenetic networks are influenced by the order of inputs. The different orders
can lead to inconsistent experimental results. Moreover, constructing a network for large datasets is
time-consuming and the network often does not include all of the input tree nodes.

Aims:
This paper
aims to propose a novel method, called as MSSD, which can construct a phylogenetic network from
gene trees by Merging Subtrees with the Same Depth in a bottom-up way.

Methods:
The MSSD first decomposes trees into subtrees based on depth. Then it merges subtrees
with the same depth from 0 to the maximum depth. For all subtrees of one depth, it inserts each subtree
into the current networks by means of identical subtrees.

Results:
We test the MSSD on the simulated data and real data. The experimental results show that
the networks constructed by the MSSD can represent all input trees and the MSSD is more stable
than other methods. The MSSD can construct networks faster and the constructed networks have
more similar information with the input trees than other methods.

Conclusion:
MSSD is a powerful tool for studying the evolutionary relationships among species in
biologyand is free available at https://github.com/xingjiajie2023/MSSD.
Stem Cell Therapy for the Management of Type 1 Diabetes: Advances and
Perspectives
Authors: Goyal Priyanshi , Malviya Rishabha 
Published: October 2023

Abstract:
Due to insulin resistance and excessive blood sugar levels, type 1 diabetes mellitus (T1DM) is characterized by pancreatic cell loss. This condition affects young people at a higher rate than any other chronic autoimmune disease. Regardless of the method, exogenous insulin cannot substitute for insulin produced by a healthy pancreas. An emerging area of medicine is pancreatic and islet transplantation for type 1 diabetics to restore normal blood sugar regulation. However, there are still obstacles standing in the way of the widespread use of these therapies, including very low availability of pancreatic and islets supplied from human organ donors, chal-lenging transplantation conditions, high expenses, and a lack of easily accessible methods. Ef-forts to improve Type 1 Diabetes treatment have been conducted in response to the disease's increasing prevalence. Type 1 diabetes may one day be treated with stem cell treatment. Stem cell therapy has proven to be an effective treatment for type 1 diabetes. Recent progress in stem cell-based diabetes treatment is summarised, and the authors show how to isolate insulin-pro-ducing cells (IPCs) from a variety of progenitor cells.
Review of Research on Dynamic Characteristics of Rolling Bearing Cages
Authors: Pan Chengyi , Li Shuhao , Zhang Jingren 
Published: October 2023

Background:
Rolling bearings are beingwidely used in various applications because of
their unique functions, but at the same time, bearing failures are becoming more and more prominent.
Among them, the bearing failure caused by cage failure also accounted for 25% of the total
proportion. With the progress of intelligent manufacturing and "Industry 4.0", the operating environment
of rolling bearings is becoming increasingly complex and variable, and higher demands
are made on the dynamic characteristics of cages. In this regard, the research on the nonlinear dynamics
of the cell under different influencing factors can help to explain better the mechanism of
dynamic, unstable motion of the cage under various influencing factors and solve the bearing life
and reliability problems caused by uneven rolling bearing cage from the perspective of design and
experimental research.

Objective:
By summarizing the influencing factors of cage dynamics characteristics, the design of
new structures, test methods, and testing devices of dynamics attributes in recent years, some valuable
conclusions were obtained, and the future development direction of rolling bearing cage dynamics
characteristics was proposed to provide a reference for researchers in related fields.

Method:
This paper reviews the patents and documents related to the dynamic characteristics of
rolling bearing cages, as well as analyzes the different influencing factors on the operational stability
of the bearings, and introduces the improved design of cage structures, as well as the test
methods and testing devices for the dynamic characteristics of cages.

Results:
Through the analysis of relevant patents and papers on rolling bearing cage dynamics,
the development status and problems of rolling bearing cage dynamics were discussed, future research
on rolling bearing cage dynamics was prospected, and design ideas and research directions
were proposed.

Conclusion:
Through the discussion of the factors influencing the cage dynamics, the optimization
of the rolling bearing cage structure design, and the study of test methods and testing devices,
it was found that the research direction of the rolling bearing cage dynamics needs to be further
expanded so that a better research method and testing devices could be researched, and a new
structure with more stable operation and higher cage life and reliability could be designed.
Plicatin C: A New Cinnamate Derivative from Mangrove-derived Fungus
Aspergillus terreus DM62
Authors: Tian Danmei , Chen Mei , Ding Wenjuan , Li Jia , Wu Shi , Hong Kui , Tang Jinshan 
Published: October 2023

Background:
Marine fungi have been proven to be a new arsenal for the discovery of
valuable secondary metabolites.

Method::
Fungus strain Aspergillus terreus DM62 was initially fermentated using solid corn medium
and PDA liquid medium. Subsequently, extensive chromatographic methods were applied to isolate
the fermentation cultures of DM62, and the chemical structures of isolate compounds were elucidated
by pectroscopic analyses and optical rotations comparison. Additionally, α-glucosidase and ATPcitrate
lyase (ACL) inhibitory activities of isolate compounds were assessed to investigate their hypoglycemic
and lipid-lowering activities.

Result::
A new cinnamate derivative, plicati n C (1), together with 18 known compounds, were isolated
from the solid and liquid state fermentations of A. terreus DM62. Activity evaluation showed that compounds
3, 6, and 8-10 exhibited stronger α-glucosidase inhibitory activities than acarbose at 400 μM,
and butenolide 3, with an IC50 value of 21.5 μM, was discovered with significant ACL inhibitory
activity for the first time.

Conclusion::
This study not only discovered a new cinnamate derivative but also found butenolides
with potent ACL inhibitory activity, which is favorable to clarify their pharmacological mechanism
in the treatment of metabolic disease.
NiO@PANI based Nanocomposites as an Advanced Functional Material:
Experimental and Computational Approach
Authors: Kumar Harish , Luthra Manisha 
Published: October 2023

Background::
Individually, metal nanoparticles (NPs) and conducting polymers show
unique properties due to small size, large surface area, and high order of conductivity. But their
combination may result in a synergistic effect in properties.

Methods::
The NiO NPs and conducting polymer Polyaniline were prepared by modified Sol-gel
and chemical oxidative methods, respectively. Powder XRD, FTIR, TEM, and UV-visible methods
were used for the structural evaluation. The computational (DFT) study was performed to
support experimental results. The NiO/Polyaniline (PANI) nanocomposites (NCs) were explored
as corrosion inhibitors, electrical conductors, and photocatalytic agents.

Results::
The NiO/Polyaniline NCs showed 91.52% corrosion inhibition efficiency at 1000 ppm
concentration. The photocatalytic activity was investigated against methylene blue dye under ultraviolet
light. The NiO/Polyaniline NCs decompose 90% of organic dye. The NCs exhibit good
conducting, corrosion inhibition, and photocatalytic activity.

Conclusion::
The metal oxide (NiO NPs) and PANI-based NCs can be used as corrosion inhibitors,
conducting material, and for the degradation of organic compounds (dyes) in impure water.
Modelo de probabilidad de ocurrencia del caracol gigante africano (Achatina fulica) para Boyacá, Colombia
Authors: Sánchez Rojas Edwin Fernando , Gómez Cedeño Maijdinayiver Mairán , Cárdenas Cárdenas María Alejandra , Gil Padilla Luz Nidia 
Published: October 2023

La capacidad invasora y la historia natural de Achatina fulica (Linnaeus, 1758) posiciona esta especie como una de las 100 especies invasoras más peligrosas a nivel mundial. Su estudio ha trascendido los límites de la investigación biológica para complementarse con análisis de predicción de los comportamientos ecológicos y la influencia de los factores sociales y económicos. El objetivo de la investigacion fue generar un modelo de probabilidad de ocurrencia para el caracol gigante africano, a escala espacial y temporal. Se recopilaron registros de presencia de la especie mediante información secundaria para el departamento de Boyacá; la información bioclimática se obtuvo a partir de la plataforma Worldclim, los indicadores sociales y económicos de cada localidad se extrajeron de la base de datos Terridata-dnp. La información se analizó por medio de software estadístico R Studio, mediante los paquetes ENMTML y ISLR. Para el modelamiento se utilizó el algoritmo de máxima entropía (Maxent); la validación del modelo se realizó con las métricas AUC, TSS, Kappa, Sorensen y Jaccard. Las variables bioclimáticas que más influyeron en el modelo de probabilidad de ocurrencia fueron temperatura, precipitación y humedad; así mismo, se determinó que las variables cobertura de acueducto y área deforestada están relacionadas estadísticamente en los municipios en donde se ha reportado la especie invasora en el departamento. En las condiciones actuales el caracol gigante africano, podría establecerse en aproximadamente 11.209,74 km; es decir, en el 47,54% del territorio boyacense; bajo las condiciones socioeconómicas de la predicción futura, en el escenario ssp1 126 la especie podría ampliar su presencia hasta el 63.25% y en el escenario ssp4 585 al 50,42%, siendo este último el más caótico se encontró que la ocupación puede ser menor al primer escenario, pero la probabilidad de ocurrencia es más alta.
Acceptance of the Apple Watch Series 6 for Telemonitoring of Older Adults With Chronic Obstructive Pulmonary Disease: Qualitative Descriptive Study Part 1
Authors: Arnaert Antonia , Sumbly Pia , da Costa Daniel , Liu Yuxin , Debe Zoumanan , Charbonneau Sylvain 
Published: October 2023

Background
The Apple Watch is not a medical device per se; it is a smart wearable device that is increasingly being used for health monitoring. Evidence exists that the Apple Watch Series 6 can reliably measure blood oxygen saturation (SpO2) in patients with chronic obstructive pulmonary disease under controlled circumstances.

Objective
This study aimed to better understand older adults’ acceptance of the Watch as a part of telemonitoring, even with these advancements.

Methods
This study conducted content analysis on data collected from 10 older adults with chronic obstructive pulmonary disease who consented to wear the Watch.

Results
Using the Extended Unified Theory of Acceptance and Use of Technology model, results showed that participants experienced potential health benefits; however, the inability of the Watch to reliably measure SpO2 when in respiratory distress was concerning. Participants’ level of tech savviness varied, which caused some fear and frustration at the start, yet all felt supported by family and would have explored more features if they owned the Watch. All agreed that the Watch is mainly a medical tool and not a gadget.

Conclusions
To conclude, although the Watch may enhance their physical health and well-being, results indicated that participants are more likely to accept the Watch if it ultimately proves to be useful when experiencing respiratory distress.
Supporting Pain Self-Management in Patients With Cancer: App Development Based on a Theoretical and Evidence-Driven Approach
Authors: Abahussin Asma A , West Robert M , Wong David C , Ziegler Lucy E , Allsop Matthew J 
Published: October 2023

Background
To inform the development of an intervention, it is essential to have a well-developed theoretical understanding of how an intervention causes change, as stated in the UK Medical Research Council guidelines for developing complex interventions. Theoretical foundations are often ignored in the development of mobile health apps intended to support pain self-management for patients with cancer.

Objective
This study aims to systematically set a theory- and evidence-driven design for a pain self-management app and specify the app’s active features.

Methods
The Behavior Change Wheel (BCW) framework, a step-by-step theoretical approach to the development of interventions, was adopted to achieve the aim of this study. This started by understanding and identifying sources of behavior that could be targeted to support better pain management. Ultimately, the application of the BCW framework guided the identification of the active contents of the app, which were characterized using the Behavior Change Technique Taxonomy version 1.

Results
The theoretical analysis revealed that patients may have deficits in their capability, opportunity, and motivation that prevent them from performing pain self-management. The app needs to use education, persuasion, training, and enablement intervention functions because, based on the analysis, they were found the most likely to address the specified factors. Eighteen behavior change techniques were selected to describe precisely how the intervention functions can be presented to induce the desired change regarding the intervention context. In other words, they were selected to form the active contents of the app, potentially reducing barriers and serving to support patients in the self-management of pain while using the app.

Conclusions
This study fully reports the design and development of a pain self-management app underpinned by theory and evidence and intended for patients with cancer. It provides a model example of the BCW framework application for health app development. The work presented in this study is the first systematic theory- and evidence-driven design for a pain app for patients with cancer. This systematic approach can support clarity in evaluating the intervention’s underlying mechanisms and support future replication.
PERBANDINGAN PERATURAN CITES 1975 PADA PERATURAN PEMERINTAH NOMOR 8 TAHUN 1999 DAN IMPLEMENTASINYA PADA KASUS DI INDONESIA
Authors: Adi Pratama Collin 
Published: October 2023

ABSTRAK
Indonesia, yang sejak bertahun tahun lalu sudah dikenal sebagai paru-paru dunia menyimpan berbagai kekayaan hayati yang melimpah ruah. Dengan kondisi seperti ini menjaga dan melindungi hutan Indonesia akan sangat berpengaruh untuk menjaga keseimbangan kehidupan dunia, karena industrialisasi, emisi karbon, dan polusi. Oleh karena itu perubahan iklim dan kenaikan permukaan laut serta berbagai bencana iklim lainnya tidak dapat terhindarkan. Dengan menjaga hutan Indonesia saja sudah sangat berpengaruh pada hal-hal yang disebutkan di atas. Selain itu adapun makhluk hidup yang ada dihutan dan perlu dijaga kelestariannya, yaitu fauna endemik yang menempati hutan hujan di Indonesia satwa-satwa ini juga berperan penting sebagai penyebar benih alami yang menjaga ekosistem di Indonesia dan diperlukannya peraturan untuk melindungi setiap tindakan yang dilakukan terhadap hutan yang ada di Indonesia khususnya terhadap perdagangan ataupun eksploitasi flora, fauna, dan kekayaan hayati lainnya.
Kata kunci: Hutan, Flora, Fauna.
ABSTRACT
Indonesia, which for many years has been known as the lungs of the world, it means Indonesian forest are excessive of biodiversity wealthiness. Under these conditions protecting and maintaining Indonesian forest will extremely affect the world life balance, because nowadays industrialization, carbon emission, and pollution. Therefore climate change and sea level rises can’t be avoided. Protecting Indonesian forest is one of the way to decrease the damage that mentioned above. In the other hand there is so much living things that exist inside Indonesian rainforest that needed to be protected, that’s the endemic fauna that live inside the Indonesian rainforest, these animals also play an important rule to protect the Indonesian rainforest as natural seed spreaders that maintain the ecosystem of Indonesian rainforest. Therefore the regulation are needed to protect every action taken against the Indonesian forest, especially against the trade and exploitation of flora, fauna, and other biodiversity.
Keywords: Forest, Flora, Fauna.
Approaches to assessing the environmental responsibility of enterprises in the industrial region
Authors: Bieloborodova Mariia , Bessonova Anna 
Published: October 2023

AbstractThe purpose of the study is the identification and forecasting of socioecological effects from the implementation of environmental responsibility by industrial enterprises at the local level. The development of methodical approaches and practical recommendations for the organizational and economic support of environmental responsibility to improve social tension in the industrial region is explored. The process of the assessment of the level of environmental responsibility of an industrial enterprise is improved based on the factors of its environmental obligation and environmental initiative. It is proposed to assess the level of environmental responsibility of enterprises not only on the basis of widely used coefficients characterizing the level of pollution, but also taking into account the level of environmental initiative of the enterprises under study, which is expressed mainly through social investments.
Authors: Linawati N.M. , Arijana I.G.K.N. , Wande I.N. , Sugiritama I.W. , Wiryawan I.G.N.S. , Ratnayanti I.G.A.D. , Wahyuniari I.A.I. , Laksmi D.A.A.S. 
Published: October 2023

Golongan darah adalah satu dari parameter identifikasi seseorang. Penggolongan darah penting diketahui dalam kaitannya dengan masalah medis maupun non medis. Permasalahan medis terkait transfusi darah dan transplantasi organ sedangkan non medis seperti kasus bayi tertukar, penculikan anak dan ragu ayah. Namun data golongan darah ternyata masih banyak belum diketahui pada siswa di Desa Ketewel. Kegiatan pengabdian masyarakat ini bertujuan untuk menentukan golongan darah ABO-Rhesus pada anak-anak SDN 3 Ketewel. Kegiatan dilaksanakan setelah berkordinasi dengan Kepala Desa, Kepala Sekolah SDN 3 serta Laboratorium Klinik Mantra Medika Ketewel. Pelaksanaan kegiatan pada bulan Agustus 2021 dengan mematuhi protokol kesehatan masa pandemic Covid-19. Hasil kegiatan didapatkan sebanyak 65 siswa terdiri atas 35 orang laki-laki dan 30 wanita dimana 100% memiliki Rhesus positif. Distribusi golongan darah ABO pada siswa laki-laki adalah 14 % A, 25% B, 62% O dan 3% AB. Sedangkan distribusi golongan darah ABO pada siswa wanita adalah 13% A, 33% B dan 47% O. Dari kegiatan dapat disimpulkan distribusi golongan darah ABO-Rhesus pada siswa SDN 3 Ketewel terbanyak adalah O, paling sedikit golongan darah AB dimana semua siswa memiliki Rhesus positif.
Kata Kunci: Identifikasi, Golongan darah ABO-Rhesus, SDN 3 Ketewel
Authors: Sugiritama I.W. , Arijana I.G.K.K. , Wiryawan I.G.N.S. , Ratnayanthi I.G.A.D. , Linawati N.M. , Wahyuniari I.A.I. , Laksmi D.A.A.S. 
Published: October 2023

Menurut Kementerian Kesehatan RI, prevalensi anemia di Indonesia pada tahun 2013 sebesar 46,3 persen, dengan prevalensi tertinggi pada kelompok lanjut usia (lansia). Masalah anemia pada lansia menjadi lebih serius karena populasi lansia cenderung meningkat dan anemia dapat meningkatkan morbiditas dan mortalitas. Anemia pada lansia disebabkan oleh kekurangan zat gizi seperti protein, zat besi, vitamin B12, asam folat dan vitamin C. Selain itu, kebiasaan mengkonsumsi makanan atau minuman yang dapat menghambat penyerapan nutrisi bisa menjadi penyebab anemia pada lansia. Tujuan dari kegiatan ini adalah untuk mengetahui kadar hemoglobin (Hb) lansia di Dusun Demulih, memberikan multivitamin dan tablet besi, serta mengedukasi tentang pencegahan anemia. Sebanyak 80 lansia ikut serta dalam pengabdian masyarakat ini. Hasil pengukuran kadar hemoglobin menunjukkan 27 lansia mengalami anemia (33,7%) dan 53 orang (66,3%) memiliki kadar hemoglobin normal. Jika dibandingkan antara pria dan wanita lansia maka wanita lebih banyak yang mengalami anemia (37,5%) dibandingkan dengan pria (28,1%). Data tersebut menunjukkan bahwa prevalensi anemia pada lansia cukup tinggi terutama pada wanita usia lanjut.
Kata kunci : anemia, hemoglobin, lansia, multivitamin, zat besi