bluuebunny/crossref_metadata_2025_split huggingface dataset:
Family History of Nonsyndromic Orofacial Clefts: A Brazilian Multicenter Study
Authors: de Barros Leonardo Pereira , Machado Renato Assis , das Neves Lucimara Teixeira , Martelli Daniella Reis Barbosa , Rangel Ana Lúcia Carrinho Ayroza , Volpato Luiz Evaristo Ricci , de Oliveira Fabrício Emanuel Soares , Farha Ana Laura Herrera , de Reis Silvia Regina Almeida , de Souza Danyele Cambraia Franco , Scariot Rafaela , Coletta Ricardo D. , Martelli‐Júnior Hercílio 
Published: January 2025

ABSTRACTObjectiveTo summarize the evidence on the relationship between hereditary family history and nonsyndromic orofacial clefts (NSOC) in patients from various Brazilian states.MethodsThis cross‐sectional multicenter study was conducted at six specialized orofacial cleft services across different regions of Brazil. The sample consisted of 1899 patients with NSOC, including cleft lip only (NSCLO), cleft palate only (NSCPO), and cleft lip and palate (NSCLP). Data were collected from clinical records between June 2023 and May 2024. Family history was classified as positive or negative, with additional details on the number of affected relatives, degree of kinship, and type of oral cleft. Statistical analysis was performed using SPSS version 27.0, with chi‐square tests and z tests to assess associations between variables, and Student's t test to compare the mean number of affected relatives among cleft types.ResultsOf 1899 patients, 52.6% had NSCLP, 24.11% had NSCPO, and 23.27% had NSCLO. NSCLP and NSCLO were more common in males, while NSCPO was more frequent in females. NSCLP and NSCLO showed similar rates of positive family history, whereas NSCPO had significantly fewer cases with hereditary links. Third‐degree relatives were the most affected across all groups.ConclusionNSCLP and NSCLO showed a higher frequency in males and more cases of positive family history compared to NSCPO. Further studies are needed to explore the genetic basis of NSOC, particularly in genetically diverse populations like Brazil.
Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth
Authors: Li Zhongze , Kim Minjae , da Silva Nascimento Jose Roberto , Legeret Bertrand , Jorge Gabriel Lemes , Bertrand Marie , Beisson Fred , Thelen Jay J. , Li‐Beisson Yonghua 
Published: January 2025

SummaryThe first step in chloroplast de novo fatty acid synthesis is catalysed by acetyl‐CoA carboxylase (ACCase). As the rate‐limiting step for this pathway, ACCase is subject to both positive and negative regulation. In this study, we identify a Chlamydomonas homologue of the plant carboxyltransferase interactor 1 (CrCTI1) and show that this protein interacts with the Chlamydomonas α‐carboxyltransferase (Crα‐CT) subunit of the ACCase by yeast two‐hybrid protein–protein interaction assay. Three independent CRISPR‐Cas9 mediated knockout mutants for CrCTI1 each produced an ‘enhanced oil’ phenotype, accumulating 25% more total fatty acids and storing up to fivefold more triacylglycerols (TAGs) in lipid droplets. The TAG phenotype of the crcti1 mutants was not influenced by light but was affected by trophic growth conditions. By growing cells under heterotrophic conditions, we observed a crucial function of CrCTI1 in balancing lipid accumulation and cell growth. Mutating a previously mapped in vivo phosphorylation site (CrCTI1 Ser108 to either Ala or to Asp), did not affect the interaction with Crα‐CT. However, mutating all six predicted phosphorylation sites within Crα‐CT to create a phosphomimetic mutant reduced this pairwise interaction significantly. Comparative proteomic analyses of the crcti1 mutants and WT suggested a role for CrCTI1 in regulating carbon flux by coordinating carbon metabolism, antioxidant and fatty acid β‐oxidation pathways, to enable cells to adapt to carbon availability. Taken together, this study identifies CrCTI1 as a negative regulator of fatty acid synthesis in algae and provides a new molecular brick for the genetic engineering of microalgae for biotechnology purposes.
Genetic Improvement of Sweet Potato: Influence of Selection Method in Early Stages of the Programme
Authors: de Oliveira Darllan Junior Luiz Santos Ferreira , Toledo Geissiane Neves , Pavan Bruno Ettore , Vargas Pablo Forlan 
Published: January 2025

ABSTRACTPlant breeding programmes: every stage of the process is crucial for the ultimate success. Currently, for sweet potato cultivation, the primary selection methods used in the initial stage of the genetic improvement programme are based on selection indices, techniques that require significant labour and time. Given this, the aim of this study was to estimate genetic parameters and compare different initial selection methods, namely, the traditional method using the selection rank index and a newly proposed methodology using visual analysis. The study comprised three experiments. The studies were conducted in an area belonging to the Faculty of Agrarian and Veterinary Sciences (FCAV) on the Jaboticabal Campus, located in the municipality of Jaboticabal, SP, Brazil. The first involved selection of the initial population conducted in summer (August–December 2021), using a randomized complete block design with two replications, evaluating 512 treatments (510 genotypes and two cultivars). Subsequent experiments in summer (December 2021–April 2022) and winter (April–September) evaluated 30 genotypes selected in the first cycle and one cultivar (Beauregard) using a randomized complete block design with three replications. Harvesting in the summer experiments occurred 120 days after planting, while in winter, it occurred at 150 days. Statistical analyses were performed on the data. The highest heritabilities were found for commercial root yield (PC) (0.69) and flesh colour (CP) (0.79). Regarding direct selection gain, the predicted gains were significant, ranging from −21.21% to 119.58%, indicating a favourable condition for selection. The estimated selection gains were higher than those achieved for PC alone. However, for general shape (FG), commercial pattern (PDC) and flesh colour (CP), the actual gains exceeded the estimated ones. The realized heritability was lower than the estimated one for commercial root yield (PC) but higher for general shape (FG), commercial pattern (PDC) and flesh colour (CP), except for commercial pattern (PDC) in winter cultivation using the index and flesh colour (CP) in summer cultivation using the visual method. In conclusion, the visual selection method is deemed viable for the initial stage of sweet potato genetic improvement.
A Novel Report of Müllerian (Vaginal/Uterine) Agenesis in a Newborn Girl With LUMBAR Syndrome
Authors: Havele Sonia A. , Horii Kimberly A. , Metry Denise W. , Lawson Ashli , Rentea Rebecca M. , Nopper Amy J. 
Published: January 2025

ABSTRACTLUMBAR syndrome is characterized by lower body segmental infantile hemangiomas, urogenital abnormalities/hemangioma ulceration, spinal cord malformations, bony deformities, anorectal malformations/arterial anomalies, and/or renal anomalies. Here we present an infant girl with LUMBAR syndrome who was also discovered to have Müllerian agenesis, defined as absent uterus or nonfunctional uterine remnants. While vaginal and uterine duplications are included among the diagnostic criteria for LUMBAR syndrome, this is the first case of associated Mullerian agenesis. This report also provides more evidence that LUMBAR syndrome be included in the spectrum of clinically overlapping disorders characterized by caudal dysgenesis, known as “recurrent constellations of embryonic malformations.”
Employees’ Perceived Overqualification and Spouses’ Family‐Work Enrichment: Examining the Role of Schedule Control and Competitive Climate
Authors: Dong Yanan , Luksyte Aleksandra , Zhang Mian , Ma Lin 
Published: January 2025

ABSTRACTResources are critical for family‐work enrichment (FWE), meaning that more resources facilitate higher FWE. This suggests that living with overqualified partners—those with surplus skills, abilities, and work experience—might increase spouses’ FWE. However, this may not always be true, as overqualified employees may lack motivation or opportunities to transfer their surplus resources to home domains, thereby reducing their spouses’ FWE. Drawing on the crossover of resources theory, this study posits that the nature of this relationship depends on employees’ schedule control or the competitive climate via social support for spouses. We examined the proposed moderated mediation model in two studies. Study 1 (N = 246) was based on a sample of hospital employees and their spouses, and Study 2 (N = 328) used matched data from employees of an information technology company and their spouses. Study 1 showed that when schedule control was higher or competitive climate was lower, perceived overqualification was positively related to spouses’ FWE through increased social support. We constructively replicated these effects in Study 2 using (a) instrumental and emotional support; (b) additional outcomes such as spouses’ job performance and work well‐being; and (c) alternative mediators and moderators. Across the two studies, our research supports the transfer of the surplus resources of overqualified employees to facilitate their spouses’ FWE in favorable work contexts.
Intricate microbial structures: The enigma of reticulated filaments
Authors: Miller Ana Zélia , Saiz‐Jimenez Cesareo 
Published: January 2025

ABSTRACTMicroorganisms inhabit all possible biosphere ecosystems, including natural and man‐made subterranean environments, which favour the growth of specialized microbial communities under near‐starvation conditions. Among the most enigmatic microbial features found in karstic and volcanic caves are reticulated filaments, consisting of long mineralized filaments forming an open‐mesh with square‐shaped or hexagonal‐shaped reticles. Despite their widespread occurrence and detailed morphological documentation, their microbial origins, formation mechanisms and ecological roles remain enigmatic. This review consolidates current knowledge on reticulated filaments, and addresses theoretical and scientific gaps on these intricate microbial structures, highlighting the association of reticulated filaments with biomineralization processes and the potential involvement of rare or uncultivated microbial taxa. Variations in sheath composition and size suggest the involvement of multiple microbial groups, potentially including bacteria, archaea and fungi. This study proposes that reticulated filaments serve as biosignatures, reflecting complex microbe–mineral interactions and local geochemical conditions. Their occurrence in environments rich in iron and manganese points to potential biogeochemical cycling roles, while their structural complexity suggests adaptive strategies for microbial survival. Future research directions are outlined and the relevance of reticulated filaments as indicators of past and present microbial activity is highlighted, with implications for understanding biomineralization, geomicrobiology and the search for life in extreme environments.
Empowering mathematics teacher educators: Exploring Artificial Intelligence‐driven mathematical tasks
Authors: Aqazade Mahtob , Mauntel Matthew , Atabas Sebnem 
Published: January 2025

AbstractWith the growing attention to generative artificial intelligence tools, particularly ChatGPT, there have been efforts to explore its implications for and applications in teacher education programs. Mathematics teacher educators (MTEs) can leverage ChatGPT as a facilitative tool to enhance preservice teachers' understanding, analyzing, and enacting of high‐leverage, effective mathematics teaching practices. One such practice is designing mathematical tasks. In this study, we, three MTEs, utilized a self‐study methodology to first highlight how our domains of knowledge informed our prompt engineering techniques given to ChatGPT to generate mathematical tasks and second, to share lessons learned from our interactions with ChatGPT during our critical friend dialogues. We primarily used our pedagogical and pedagogical content knowledge to craft prompts. Our prompt engineering techniques involved revising, generating, or editing. The lessons learned underlined the different conceptualizations of what constitutes a task, and the importance of articulating and navigating the learning goal throughout ChatGPT interactions. Our findings have implications for fostering MTEs' knowledge of using ChatGPT to generate mathematical tasks and ways of supporting current and future teachers in using ChatGPT in their classrooms.
“I didn't know it formally”: Shifting work and intended influences of informal teacher leaders in the third year of mathematics professional development
Authors: Brown Karie , Auslander Susan Swars , Vo Michael Cao Hung , Bingham Gary E. , Fuentes Debra S. 
Published: January 2025

AbstractThe development and use of elementary mathematics specialists (EMSs) is an indispensable approach for improving mathematics instruction in schools. This holistic singular‐case study explored the teacher leadership of 26 EMSs across a three‐year period as they developed in their informal teacher leader role during a five‐year mathematics professional development program. To examine participants' teacher leader work and intended influences of these efforts, quantitative and qualitative data were collected. Findings of a teacher leader record show the prevalence, variability, audiences, and shifts in their teacher leader efforts, with the interview data illuminating complexity and extensions. In their advocacy for ambitious and equitable instruction, the EMSs sought to support students, families, teachers, administrators, and community members in particular ways. Shifts were observed as they became more comfortable with their roles and responsibilities as teacher leaders. They were increasingly positioned as holders of expertise within communities of practices in their schools, contributing to more influence in their settings. EMSs reported not only supporting teachers through leading professional development but advocating for students and best practices informally in ad hoc hallway conversations. Notably, the EMSs recognized expertise in fellow teachers and sought to elevate them as leaders.
Effectiveness of clindamycin-based exposure strategies in experimental mature staphylococcal biofilms
Authors: van Dun S. C. J. , Verheul M. , Pijls B. G. C. W. , Scheper H. , van der Does A. M. , Nibbering P. H. , de Boer M. G. J. 
Published: January 2025

ABSTRACT

Due to increasing antimicrobial resistance and side effects caused by current standard antimicrobial regimens used for treatment of prosthetic joint infection (PJI), alternative options are urgently needed. We aimed to investigate the effect of clindamycin in different exposure strategies against
Staphylococcus aureus
in an
in vitro
mature biofilm model. In short, 7-day biofilms were generated on polystyrene plates and titanium–aluminum–vanadium discs using a clinical
S. aureus
PJI isolate. Next, biofilms were exposed to clindamycin according to four strategies: single 24-h exposure; prolonged 48- or 72-h exposure; repeated 24-h exposures during a 4-day period, and sequential exposures of initial 24-h rifampicin-based therapy followed by 24-h exposure to clindamycin. The remaining bacterial load (colony-forming unit [CFU]/mL) after antibiotic exposure was assessed. Confocal laser scanning and atomic force microscopy were applied to evaluate the biofilm structure. Single exposure to clindamycin for 24 h or prolonged up to 72 h did not result in any relevant reduction in bacterial load. Repeated 24-h exposures demonstrated relevant reductions of >3 log CFU/mL at clindamycin concentrations ≥16 mg/L for the 3rd and 4th consecutive doses. Sequential rifampicin–ciprofloxacin combination exposure followed by clindamycin showed bacterial load reductions of 3- to 4-log CFU/mL, similar to continued rifampicin–ciprofloxacin exposure. This was achieved for concentrations equivalent to levels achieved after standard dosing of clindamycin in clinical practice. These experimental findings support that clindamycin monotherapy is not an optimal choice when starting antimicrobial treatment of PJI but that later on, rifampicin may be safely switched to clindamycin in patients with PJI who do not tolerate prolonged rifampicin-based treatment.

IMPORTANCE

Rifampicin—in combination with another antibiotics—is recommended in all guidelines as first choice treatment of prosthetic joint infections (PJIs), despite adverse interactions and side effects associated with this antibiotic. In a search for alternative approaches, the switch to clindamycin in patients after rifampicin-based antibiotic treatment was found to be effective in some recent observational clinical studies. In our in vitro study, we determined the effect of clindamycin on
Staphylococcus aureus
in mature biofilms, to obtain further insight. Our study showed that clindamycin was effective in reducing mature biofilm-residing
S. aureus
after initial exposure to rifampicin–ciprofloxacin, while it was not effective as first treatment. These in vitro findings provide evidence for the hypothesis that rifampicin–ciprofloxacin can be successfully switched to clindamycin monotherapy in PJI patients in a later phase of treatment.
Microbiome characterization of two fresh pork cuts during production in a pork fabrication facility
Authors: Asmus A. E. , Gaire T. N. , Schweisthal K. J. , Staben S. M. , Noyes N. R. 
Published: January 2025

ABSTRACT

The goal of this study was to characterize the microbial profile of two different fresh pork cuts, bootjack (BJ) trim and tenderloin (TL), through a 16S rRNA sequencing workflow developed specifically for investigating low-biomass fresh meat within a commercial production schedule. Additionally, this study aimed to determine a baseline
Salmonella
prevalence and enumeration profile across these two fresh pork cuts. Results showed that microbiome diversity was different between the BJ and TL, and also differed significantly by processing date. The relative abundance of key bacterial genera associated with food safety and spoilage was also different between the two meat types. However, over the course of the production shift, changes in the meat microbiome were limited in both the BJ and TL. The crude prevalence and enumerated burden of
Salmonella
were lower than what has been previously reported in similar fresh pork cuts, and all of the
Salmonella
-positive samples occurred on just two processing windows of 1–2 days each. Taken together, the results of this study suggest that the microbial profile of two fresh pork cuts is significantly different even within the same plant at the same time points, and that day-to-day variability within the production process likely influences both the fresh pork microbiome and
Salmonella
profile of these two meat types.

IMPORTANCE

Modern pork processing involves a series of processes that begin with the handling and transport of the live animals, proceed through harvest and fabrication, and end with the packaging and distribution of fresh pork to the consumer. Each step in this process can alter the microbial community of fresh pork and influence the meat’s safety and shelf life. However, little is known about the microbial ecology of individual, unprocessed pork cuts and if the diversity of the meat microbiome remains consistent throughout a production schedule. Additionally, the crude prevalence and enumeration of
Salmonella
have not been well established for individual fresh pork cuts throughout a production schedule. A more thorough understanding of the microbial profile at different stages of pork production will help processors determine processing steps that impact the microbial characteristics of fresh pork. This insight will help processors implement targeted intervention strategies to enhance food safety and quality.
Reducing outpatient wait times through telemedicine: a systematic review and quantitative analysis
Authors: Capodici Angelo , Noci Francesca , Nuti Sabina , Emdin Michele , Dalmiani Stefano , Passino Claudio , Hernandez-Boussard Tina , Giannoni Alberto 
Published: January 2025

ObjectivesPopulation ageing and the rise in chronic diseases place continual stress on healthcare systems. Scarce resources often impede equitable access to healthcare, particularly in rural areas, resulting in prolonged waiting times and heightened risks of morbidity and mortality. Telemedicine has emerged as a promising solution, offering remote and equitable care that could potentially bridge access gaps and enhance health outcomes. This systematic review aims to quantitatively examine the impact of telemedicine implementation on waiting times, defined as the time passed from the booking of a visit for an outpatient to the administration of the service.DesignA systematic review was conducted using studies on telemedicine interventions that specifically addressed waiting times. Bias assessment was performed with three tools: ROBINS-I (“Risk of Bias In Non-Randomized Studies of Interventions”), AXIS ("Appraisal tool for Cross-Sectional Studies") and RoB-2 ("Risk of Bias-2"). A weighted mean approach was used to synthesise results, with medians synthesised using a median approach.Data sourcesArticles in English were retrieved from the PubMed and Scopus databases.Eligibility criteriaStudies were excluded if they did not specifically address waiting times related to telemedicine interventions. Only studies that considered waiting times defined as the time passed from the booking of a visit for an outpatient to the administration of the service and any telemedicine intervention were included.Data extraction and synthesisA total of 53 records were included, encompassing 270 388 patients in both the experimental and control groups. The weighted mean reduction in waiting times was calculated, and bias was assessed. No record was evaluated to be at high risk of bias, with 69.8% of studies evaluated at low risk and 26.4% at moderate risk (3.8% were surveys). Results were synthesised using a weighted mean approach for studies reporting means, and a median approach for studies reporting medians.ResultsOverall, a weighted mean reduction of 25.4 days in waiting times was observed. Focusing on clinical specialties (n=114 042), the weighted mean reduction amounted to 34.7 days, while in surgical patients (n=156 346), telemedicine was associated with a weighted mean of 17.3 days saved.ConclusionsThe implementation of telemedicine solutions may significantly improve waiting times, potentially leading to more efficient and equitable healthcare systems.PROSPERO registration numberCRD42023490822.