bluuebunny/crossref_metadata_2025_split huggingface dataset:
MRI-based cell tracking of OATP-expressing cell transplants by pre-labeling with Gd-EOB-DTPA
Authors: Bhattacharyya Tapas , Mallett Christiane , Shapiro Erik M 
Published: December 2023

Abstract
Purpose
A critical step in cell-based therapies is determining the exact position of transplanted cells immediately post-transplant. Here, we devised a method to detect cell transplants immediately post-transplant, using a clinical gadolinium-based contrast agent. These cells were detected as hyperintense signals using a clinically familiar T1-weighted MRI protocol.
Procedures:
HEK293 cells were stably transduced to express human OATP1B3, a hepatic organic anion transporting polypeptide that transports Gd-EOB-DTPA into cells that express the transporters, the intracellular accumulation of which cells causes signal enhancement on T1-weighted MRI. Cells were pre-labeled prior to injection in media containing Gd-EOB-DTPA for MRI evaluation and indocyanine green for cryofluorescence tomography validation. Labeled cells were injected into chicken hearts, in vitro, after which MRI and cryofluorescence tomography were performed in sequence.
Results
OATP1B3-expressing cells had substantially reduced T1 following labeling with Gd-EOB-DTPA in culture. Following their implantation into chicken heart, these cells were robustly identified in T1-weighted MRI, with image-derived injection volumes of cells commensurate with intended injection volumes. Cryofluorescence tomography showed that the areas of signal enhancement in MRI overlapped with areas of indocyanine green signal, indicating that MRI signal enhancement was due to the transplanted cells.
Conclusions
OATP1B3-expressing cells can be pre-labeled with Gd-EOB-DTPA prior to injection into tissue, affording the use of clinically familiar T1-weighted MRI to robustly detect cell transplants immediately after transplant. This procedure is easily generalizable and has potential advantages over the use of iron oxide based cell labeling agents and imaging procedures.
Changes and determinants of pneumococcal vaccine uptake in Ethiopia
Authors: Mamo Biniyam Tedla , Geram Ferehiwot Gebrehiwot , Getnet Kebron Yhenehu , Bonger Zelalem Tazu 
Published: December 2023

Abstract
Background:
Pneumococcal pneumonia is one of the most common causes of severe pneumonia and pneumonia-related mortality globally. It is one of the top five causes of mortality and mortality in children under five in Ethiopia. Vaccinating children reduced the disease burden of pneumonia and death-related pneumococcal infection for both child and adult populations. Pneumococcal Pneumonia is one of the vaccine-preventable diseases.
Objective: To assess how pneumococcal vaccine uptake changes over time and to identify factors associated with uptake of the pneumococcal vaccine.
Data: This study was conducted based on secondary data obtained from the Ethiopian Demographic and Health Surveys (EDHS) conducted in 2016 and 2019, two nationally representative surveys. A total of 1929 and 1008 children of aged 12–23 months were included res from the 2016 and 2019 Demographic and Health Surveys respectively.
Method: The Percentage change in pneumococcal vaccine (PCV) coverage was used to quantify the degree of change over time. To identify the significant factors associated with the uptake of PCV vaccines, a multilevel ordinal logistic regression model was used. All statistical tests were run with a significance threshold of 5%.
Result:The percentage of children aged 12-23 months who received complete doses of PCV vaccination increased from 49.1% in 2016 to 59.8% in 2019 with a significant 21.8% increase [95% CI: 9.8-35.2]. Children in rural areas are 69% less likely to receive more doses of PCV vaccinations than those who live in urban areas (AOR=0.307, 95% CI: 0.127 - 0.742). Second-or higher-order births are associated with higher uptake of doses of PCV vaccinations (AOR=2.519, 95% CI: 1.143-5.548]). Compared to children born at home, children born in health facilities were 2.35 times more likely to receive greater doses of the PCV vaccination (AOR=2.350, 95% CI: 1.132-4.882). Children born from the women who received more ANC visits were significantly more likely to receive complete PCV coverage.
Conclusion: We observed a significant increase in the uptake of PCV vaccines across the two survey years even though Ethiopia is still far away from achieving national immunization goals. Our results also show that place of residence, birth order, place of delivery, ANC visits, and region were significantly associated with pneumococcal vaccine uptake.
The Relationship Between Physical Activity, Nutritional Status, and Sarcopenia in Community- Dwelling Older Adults with Type 2 Diabetes: A Cross-Sectional Study
Authors: Ji Chun-hui , Huang Xiao-qin , Li Yue , Luo Zhao-hui , Muheremu Aikeremujiang 
Published: December 2023

Abstract

Aim
This study was conducted in Urumqi, Xinjiang, to assess the prevalence of sarcopenia and to determine the relationship between physical activity, nutritional status, and sarcopenia among community-dwelling patients with type 2 diabetes mellitus.
Methods
A total of 408 elderly individuals (aged > 60) after physical examination participated in this study in the Urumqi, Xinjiang community. Nutritional status, physical activity, and body composition served as explanatory points in identifying sarcopenia based on the 2019 Asian Sarcopenia criteria. To further explore the influences on sarcopenia, univariate and binary logistic regression analyses were conducted.
Results
Among the 408 patients, 84 (20.6%) had sarcopenia, with a frequency of 12.6%, 32.1%, and 51.9% in those aged 60–70, 71– 80, and 81 or older respectively. The frequency increased significantly with age. Adjusting for variables, the study found that FFM of the Left Leg (OR: 9.111, 95% CI: 1.342 ~ 61.869, P = 0.024), FFM of the Right Arm (OR: 35603.510, 95% CI: 160.022 ~ 7921444.104, P < 0.001), Age (OR: 1.246, 95% CI: 1.031 ~ 1.505, P = 0.023), ASMI (OR: 0.000, 95% CI: 0.00 ~ 0.01, P < 0.001), Fasting Blood Glucose (OR: 1.649, 95% CI: 1.066 ~ 2.550, P = 0.025), and Post-Prandial Blood Glucose (OR: 1.455, 95% CI: 0.999 ~ 2.118, P = 0.025) were independent risk factors for sarcopenia. An increase in MNA score (OR: 0.398, 95% CI: 0.244 ~ 0.6500, P < 0.001) and walking energy expenditure (MET-min) (OR: 0.998, 95% CI: 0.996 ~ 0.999, P = 0.001) reduced the frequency of sarcopenia.
Conclusion
This study shows that an age- related increase in skeletal muscle mass index, a decrease in FFM of the right arm, higher postprandial blood glucose, a higher MNA score, and increased walking energy expenditure (MET-min) are significantly associated with combined sarcopenia.
The Impact of Varied Durations of Sleep Deprivation on Anxiety and Depressive-like Behaviors in Mice
Authors: Cao Dandan , Geng Xue , Xiaoling Fang , Yi Shaoqiong , Pan Huashan , Zhao Yi 
Published: December 2023

Abstract
Sleep disorders and depression often coexist, and preclinical studies using animal models are crucial for improving the treatment of depression. However, previous studies have not compared the effects of different durations of sleep deprivation on depression, nor have they explored the optimal time period for treatment following sleep deprivation. In our study, we aimed to investigate the mechanisms through which sleep deprivation induces anxiety- and depression-like behaviors in mice and determine the most effective timeframe for treatment. Additionally, we briefly examined the relationship between inflammation and the pathophysiology of depression. To create a model of depression, we utilized adult male C57BL/6J mice and subjected them to sleep deprivation. Following the sleep deprivation period, the mice were allowed to sleep normally for either 14 or 21 days. We employed five behavioral tests to assess anxiety and depression-like behaviors. Furthermore, we conducted H&E staining and Nissl staining to examine cell morphology and neuronal changes. Real-time fluorescence quantitative PCR (RT-qPCR) was employed to measure mRNA levels of clock genes, Silent information regulator 6 (Sirt6), High mobility group box-1 (Hmgb1) , and inflammatory factors. Our study demonstrates that sleep deprivation serves as a reliable mouse model for depression, with 7 or 14 days of sleep deprivation considered optimal. Moreover, the recommended duration for effectively treating sleep deprivation-induced depression in animal models is 14 days. Limited evidence suggests that sleep deprivation may impact the Sirt6/Hmgb1 pathway and influence the transcription of clock genes, thereby activating inflammation in the medial prefrontal cortex (mPFC) region of mice.
Development, implementation, and evaluation of an app-based functional social relationships promotion program for cancer patients: A protocol study
Authors: Bahrami Masood , Harchegani Hossein Beigi , Zarei Bahare 
Published: December 2023

Abstract
Background

Cancer is a substantial public health issue in Iran and around the world. According to data consolidated from several studies, a significant percentage of cancer patients reported high levels of social problems, such as interpersonal relationships. This study aims to develop, implement, and evaluate a functional social relationships promotion program for cancer patients.
Methods/Design
This is a development‐applied study with four phases. Based on the planning model of Ewles and Sminett (2010), by reviewing the extensive literature and domestic and foreign databases and app markets, the need for the functional aspect of cancer patients' social relationships was identified in the first phase. A panel of experts will then prioritize these needs, and the initial draft of the program will be finalized, and the appropriate content to be presented through the app will be extracted. The required app features and architecture will be determined in the second phase, and the app will be developed. Next, the developed app will be made available to the designated contacts (20 patients and three experts), and its quality and usability will be assessed. In the fourth phase, a quasi-experimental study intervention and its impact on the functional aspect of social relationships will be evaluated.
Discussion
Expectedly, study findings will address several of the needs associated with social relationships in cancer patients by providing an app-based intervention program to improve functional aspects of social relationships. Future research and the development of policies, education, and interventions for cancer patients can benefit from this program.
Classification of Long-term Disease Patterns in Inflammatory Bowel Disease and Analysis of their Associations with Adverse Health Events: a UK Biobank Cohort Study
Authors: Li Fan , Chang Yu , Wang Zhaodi , Wang Zhi , Zhao Qi , Han Xiaoping , Xu Zifeng , Yu Chanjiao , Liu Yue , Chang Shiyu , Li Hongyan , Hu Sileng , Li Yuqin , Tang Tongyu 
Published: December 2023

Abstract

Background
With existing researches identifying an increased risk of long-term conditions (LTCs) among Inflammatory Bowel Disease (IBD) patients, yet there is a lack of exploration into the patterns of comorbidity and prognostic risks for IBD patients with multiple morbidities.
Methods
We included 8,305 participants who self-reported having IBD (comprising UC and CD) and utilized latent class analysis (LCA) to create optimal categories of LTC combinations for UC and CD patients with additional LTCs. Using Cox proportional hazards models, we compared the all-cause mortality risk over a 16-year follow-up among UC and CD patients within different LTC categories, both without LTCs and with the addition of one LTC, risks of major adverse cardiovascular events (MACE), and the risk of IBD-related surgeries.
Results
A total of 5,617 participants reported having two or more LTCs, with the LCA method identifying three prevalence categories among CD patients, and four prevalence categories among UC patients. The highest mortality rate among CD patients was found in category 3: (HR 1.789, 95% CI (1.439–2.224)), and the highest risk of MACE was also in category 3: (HR 11.432, 95% CI (9.332–14.005)), with hypertension being the distinguishing characteristic of this category, and the highest rate of IBD-related surgeries being associated with pain in category 1: (HR 1.217, 95% CI (0.983–1.506)). Among UC patients, the highest mortality rate was in category 3: (HR 2.221, 95% CI (1.837–2.684)), with the highest MACE risk found in category 3: (HR 6.422, 95% CI (5.659–7.288)), and the highest rate of IBD-related surgeries being associated with pain, also in category 3: (HR 1.218, 95% CI (1.041–1.425)).
Conclusion
The risk of adverse health outcomes in IBD patients is closely associated with multimorbidity patterns, underscoring the need to fully consider multimorbidity patterns in the assessment, management, and treatment strategies for IBD.
A novel adaptive image privacy protection method based on Latin square
Authors: Chen Yucheng , Huang Huiqing , Tang Chunming , Wei Weiming 
Published: December 2023

Abstract

Recently, there has been a rise in image encryption methods using Latin squares. However, a significant portion of these methods relies on fixed key sequences, making them vulnerable to known plain image attacks (KPA) and chosen plain image attacks (CPA). To solve this, a novel adaptive image encryption scheme is proposed, which is based on the fractional order L$ \ddot{u} $ system and Latin squares. Specifically, the sum of each bit plane and the pixel value sum of the plain image are, respectively, used to select Latin squares and chaotic sequences in the proposed method, which is an effective approach for resisting the KPA and CPA. Moreover, using the fractional order L$ \ddot{u} $ system, the proposed method generates self-orthogonal Latin squares (SOLSs) to scramble the bit plane of the plain image in bit-level, followed by diffusing the scrambled image in pixel-level to boost the encryption’s robustness. The study compares the proposed method with state-of-the-art image encryption schemes and finds that it is superior in terms of usability, security, and efficiency. This method is highly promising for applications that require strong and efficient image encryption.
2020 MSC: 68P25
Insights into efficient electricity generation performance using starch at pH 9.0 based on Enriched exoelectrocigens
Authors: Gao Sheng-Chao , Jiang Hui-Chun , Zhang Zhen , Li Rui-Tao , Ren Xiang-Peng , Yao Shu-ya , Liu Ying , Gao Tianpeng 
Published: December 2023

Abstract
Background
Massive production of starch wastewater from various sources without any treatment might have an adverse effect on the environments. Starch as the common macromolecule in the starch wastewater can be used as the substrate of the exoelecrocigens in Microbial fuel cells (MFCs). However, their low bio-energy production rate limit their application in the real world. Therefore, it is necessary to find cultures with highly efficient electricity-producing capability. The mixed cultures (EHN) with electrochemical activity and ability to degrade starch were enriched by soluble starch in this study. we investigated the influence of pH on microbial community diversity and the electrochemical behavior of MFC for sustainable bio-electricity generation.
Results
EHN can grow in the range from pH 5.5 to pH 9.5 and the performance of electricity generation of EHN at pH 5.5, 7.2, 8.0, 8.5 till 9.5 was investigated by means of the three-electrode system and MFC system. The results showed that the electricity generation performance of EHN at pH9.0 using starch was above 22.2% higher than 548.24 µA cm− 2 at 7.2 and even 32.6% higher than at pH 5.5. Especially, the electricity generation ability of EHN has no obvious difference with pH increased to pH 8.5 until pH9.5, which is much higher than that Geobacter sulfurreducens using acetate at pH9.5. Meanwhile, the maximum power density of EHN at pH 9.0 using MFC system was 77.9% higher than that at pH 7.2. The community diversity data demonstrated that Bacillus was the unique genus of EHN at pH 9.0. From the HPLC analysis it can be seen that the degradation of starch and acetate production at pH 9.0 were faster than that at pH 5.5 and pH 7.2, which resulted in more biomass from the electrode and enhanced electricity generation.
Conclusion
This study has demonstrated that the performance of MFC depends on the pH and on the diversity of the constituent microbial communities. The performance of MFCs using starch can be improved when the pH was alkali-resistant. This study will provide a promising pathway for starch wastewater treatment using MFC technique at around pH 9.0.
Transcutaneous Auricular Vagus Nerve Stimulation Modulates Masseter Muscle Activity, Pain Perception, and Anxiety Levels in University Students: A Double-Blind, Randomized, Controlled Clinical Trial
Authors: Ferreira Luciano Maia Alves , Generoso Laura Pereira , Capello Maria Gabriela Maziero , Scoz Robson Dias , Silva Josie Resende Torres da , Silva Marcelo Lourenço da , Brites Ricardo , Fraião Gonçalo , Pereira Gonçalo , Fernandes Henrique 
Published: December 2023

Abstract
Background
This double-blind, randomized, controlled clinical trial investigated the impact of transcutaneous auricular vagus nerve stimulation (TAVNS) on masseter muscle activity, pressure pain threshold (PPT), and anxiety levels in university students with elevated anxiety.
Methods
Forty-two participants meeting inclusion criteria were randomly assigned to either true TAVNS or sham TAVNS groups. Various parameters, including masseter muscle electromyographic (EMG) signals, PPT, and Beck Anxiety Inventory (BAI) scores, were assessed before pretreatment, immediately after the intervention week, and two weeks follow-up.
Results
True TAVNS significantly reduced both left and right masseter activation during resting mandibular position, persisting for two weeks post-intervention. Additionally, TAVNS induced a lasting decrease in both left and right masseter PPT, indicative of altered pain perception. Notably, BAI scores showed a substantial reduction, emphasizing TAVNS as a potential intervention for anxiety, with effects maintained at the two-week follow-up.
Conclusion
This study provides comprehensive insights into the multifaceted effects of TAVNS on physiological and psychological aspects associated with anxiety in university students. The promising results underscore TAVNS as a potential neuromodulatory intervention for anxiety-related conditions, warranting further research and clinical exploration.
Trial registration
Brazilian Registry of Clinical Trials (ReBEC) number RBR- 4s4kt2r, retrospectively registered, 09/14/2023.
Interaction between HBsAg response and the prognosis of patients with HBV-associated HCC receiving anti-PD-1/PD-L1
Authors: Zhang Xia , Xu Qing , Ma Qin-Xia , Yang Yonglin 
Published: December 2023

Abstract
Background:The use of programmed death receptor-1 (PD-1) and programmed death ligand 1 (PD-L1) inhibitors has become widespread in the treatment of advanced hepatocellular carcinoma (HCC). However, the role of hepatitis B virus( HBV) as a major pathogenic factor in HCC has garnered significant attention regarding the efficacy of anti-PD-1/PD-L1 therapy for HBV-related HCC(HBV-HCC), yet effective predictive biomarkers remain lacking.
Method: The present study retrospectively collected data from 76 patients with HBV-HCC who were diagnosed and treated at Taizhou People's Hospital Affiliated to Nanjing Medical University between January 2015 and August 2023. The collected data included demographic characteristics and blood biochemical parameters, while the levels of hepatitis B surface antigen(HBsAg) during treatment were also recorded. The primary endpoints of this study were progression-free survival (PFS) and overall survival (OS), while the secondary endpoints included HBV reactivation and associated hepatitis. Continuous variables were compared using independent sample t-tests, categorical variables were compared using chi-square tests, and overall survival was analyzed using Kaplan-Meier curves and log-rank tests.All patients tested positive for serum HBsAg prior to treatment and had received antiviral therapy. After excluding confounding factors, the patients were categorized into two groups based on their receipt of anti-PD-1/PD-L1 therapy: the experimental group (administered with anti-PD-1/PD-L1) and the control group (not administered with anti-PD-1/PD-L1). The experimental group was further divided into two subgroups: the subgroup exhibiting increasing levels of HBsAg and the subgroup displaying decreasing levels of HBsAg.
Result: A total of 76 patients with HBV-HCC were enrolled in this study. The general information between the two groups was compared, and no statistically significant difference was observed (P>0.05). However, there were significant statistical differences in PFS and OS between the experimental group and the control group (t=5.062, p<0.001; p=0.039<0.05, respectively). The subgroup analysis conducted within the experimental group further revealed a statistically significant difference in OS (p=0.027<0.05). Moreover, HBV reactivation occurred without any significant difference observed in the incidence rates of HBV-associated hepatitis (4.7% vs 9.1% and 4.65% vs 3.03%, respectively).
Conclusion:In patients with HBV-HCC undergoing PD-1/PD-L1 inhibitor treatment, a decline in HBsAg levels can potentially serve as an indicator of enhanced tumor response and prolonged survival, while also not increasing the incidence of HBV reactivation and liver damage, thereby highlighting favorable safety profiles. Our study further suggests that PD-1/PD-L1 inhibitors may facilitate the reduction in HBsAg levels; however, additional investigations are warranted to elucidate the underlying mechanisms.
Molecular Mechanism and Therapeutic Effect of Soybean Consumption on Breast Cancer Patients
Authors: Balaei Elham , Kavand Zahra , Hanachi Parichehr , Taleahmad Sara 
Published: December 2023

Abstract
Breast cancer is the leading cause of cancer death in women and is the most common type of cancer among them. Research shows that soybeans contain powerful compounds such as isoflavones and phytoestrogens, which may play an important role in the treatment and prevention of cancer, especially breast cancer. The aim of this study was to investigate the molecular mechanisms underlying the effects of soy on breast cancer treatment using bioinformatics and transcriptomic data analysis. The search resulted in four mRNA microarray datasets (GSE9936, GSE63205, GSE50705, GSE58792). We identified differentially expressed genes (DEGs) by using. Common genes between studies were identified using Venn diagrams, and breast cancer-specific genes were extracted from differentially expressed genes using the GEPIA database. Additionally, GEO analysis and pathway enrichment of DEGs were performed using the DAVID database. In our study, the "cell cycle pathway" was introduced and investigated as an important pathway in this cancer, which is associated with soybean consumption and down-regulated genes such as CDC20, CCNB1, CDC6, MAD2L1, CCNA2, TTK, MCM4, CDC25C, MCM2, and ESPL1. Furthermore, "G2-M checkpoint" and "mutant p53" were from the pathways that have decreased activity with soy consumption. (PI3K)/Akt signaling pathway and epithelial-mesenchymal transition were down-regulated in breast cancer, and in our study up-regulated by soy and its extracts. Overall, the results of this study showed that soy compounds may be an important therapeutic target in the treatment of breast cancer, as they alter the expression of genes effective in this disease.