bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Regulation of the
Bordetella bipA
Gene: Distinct Roles for Different BvgA Binding Sites
Authors: Deora Rajendar 
Published: November 2002

ABSTRACT

The BvgAS signal transduction system of
Bordetella
controls an entire spectrum of gene expression states in response to differences in environmental conditions. In particular, the
Bordetella
Bvg-intermediate-phase gene
bipA
displays a complex regulatory pattern in response to various concentrations of modulators. Expression of
bipA
is low in the absence of modulating signals, maximal at intermediate concentrations of modulators, and near background levels at high concentrations of modulators.
bipA
is regulated at the transcriptional level, and the
bipA
promoter contains multiple BvgA binding sites present both upstream and downstream of the transcriptional initiation site. In vivo transcriptional analyses, utilizing several mutant promoter fusions to the reporter enzyme β-galactosidase, suggest that the upstream binding site IR1 is essential for expression and that the downstream binding sites IR2 and IR3 are involved in transcriptional repression. Mutations of IR2 or IR3 convert the expression profile of
bipA
from that of a Bvg-intermediate-specific-phase gene to that of a Bvg
+
-phase gene. To gain insight into the mechanism responsible for differential
bipA
regulation, DNase I protection studies were conducted with various mutant promoters. These analyses suggest that IR1 and IR2 function as core binding sites and are the primary determinants for the phosphorylation-induced oligomerization of BvgA to the adjacent regions.
The Protein Encoded at the 3′ End of the Serine Protease Gene of
Aeromonas sobria
Functions as a Chaperone in the Production of the Protease
Authors: Nomura Tomohiko , Fujii Yoshio , Yamanaka Hiroyasu , Kobayashi Hidetomo , Okamoto Keinosuke 
Published: November 2002

ABSTRACT

For the successful production of
Aeromonas sobria
serine protease (ASP), open reading frame 2 (ORF2) protein, encoded at the 3′ end of the protease operon, is required. In this study, we examined the action of ORF2 protein. The results showed that the protein associated with ASP in the periplasm and helped ASP to form an active structure.
Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in
Pseudomonas mendocina
and the TodST System from
Pseudomonas putida
Authors: Ramos-González María-Isabel , Olson Monica , Gatenby Anthony A. , Mosqueda Gilberto , Manzanera Maximino , Campos María J. , Víchez Susana , Ramos Juan L. 
Published: November 2002

ABSTRACT

The
tmoABCDEF
genes encode the toluene-4-monooxygenase from
Pseudomonas mendocina
KR1. Upstream from the
tmoA
gene an open reading frame,
tmoX
, encoding a protein 83% identical to TodX (
todX
being the initial gene in the
todXFC1C2BADEGIH
operon from
Pseudomonas putida
DOT-T1E) was found. The
tmoX
gene is also the initial gene in the
tmoXABCDEF
gene cluster. The transcription initiation point from the
tmoX
promoter was mapped, and the sequence upstream revealed striking identity with the promoter of the
tod
operon of
P. putida
. The
tod
operon is regulated by a two-component signal transduction system encoded by the
todST
genes. Two novel genes from
P. mendocina
KR1,
tmoST
, were rescued by complementation of a
P. putida
DOT-T1E
todST
knockout mutant, whose gene products shared about 85% identity with TodS-TodT. We show that transcription from P

tmoX

and P

todX

can be mediated by TmoS-TmoT or TodS-TodT, in the presence of toluene, revealing cross-regulation between these two catabolic pathways.
Significance of radiation exposure from work‐related chest X‐rays for epidemiological studies of radiation workers
Authors: Cardarelli J. , Spitz H. , Rice C. , Buncher R. , Elson H. , Succop P. 
Published: November 2002

AbstractBackgroundPrevious epidemiologic studies of workers at nuclear weapons facilities have not included X‐ray exposures as part of the occupational radiation exposure. The research objective was to determine the contribution of work‐related chest X‐ray (WRX) exposure relative to the cumulative occupational radiation exposure.MethodsCases and controls were identified from a cohort of workers whose employment began as early as 1943. Medical records for 297 subjects were used to determine the bone marrow dose from their X‐ray examinations. Individual dose data, however, were only available for 45 workers. Bone marrow dose estimates were calculated by converting the entrance‐skin‐exposure (from X‐ray procedures) and occupational exposure (from monitoring data) to dose.ResultsStereoscopic photofluorography delivered a bone marrow dose nearly 100 times that delivered by today's chest X‐ray technique. Photofluorography was the predominant radiation source during the 1940s and 1950s. The cumulative WRX dose was, on average, 50 times their occupational doses. No correlation between occupational and WRX dose was found, but may be due to the small study size and incomplete dose data.ConclusionsThese findings illustrate the importance of including WRX doses in retrospective epidemiological studies of radiation workers, especially if photofluorographic chest X‐rays were performed and occupational exposure to ionizing radiation is low. Am. J. Ind. Med. 42:490–501, 2002. Published 2002 Wiley‐Liss, Inc.
An active ring X and haploinsufficiency of SHOX contribute to short stature, congenital anomalies, and developmental delay in a female
Authors: Shago Mary , Sgro Michael , Barozzino Tony , Antinucci Daniel , Chakraborty Pranesh , Chitayat David , Teshima Ikuko 
Published: November 2002

AbstractWe report on a female infant with short stature and mesomelic limb shortening, multiple congenital abnormalities, developmental delay, and Rieger anomaly. Cytogenetic analysis revealed a complex rearrangement of the sex chromosomes in this patient. In addition to a normal X chromosome, a derivative Y [der(Y)] chromosome composed of X and Y material and a ring X [r(X)] were present. Consistent with the fact that this infant had normal female genitalia, the SRY gene was not detected in the Y chromosome portion of the der(Y). By fluorescence in situ hybridization (FISH), XIST was present on the normal X and the r(X), but not on the der(Y). The normal X was late replicating (inactive) and the r(X) early replicating (active) in all lymphocyte metaphases examined. As the X chromosome material on the der(Y) cannot be inactivated, the unusual skew of activation toward the r(X) presumably resulted in the least amount of functional disomy of X‐linked genes in the cells of this patient. Deletion of one copy of the SHOX gene was detected in this patient. Haploinsufficiency of this gene is known to be correlated with short stature and mesomelic limb shortening. © 2002 Wiley‐Liss, Inc.
Effect of the miscibility between a silane and a sizing agent on silanol condensation
Authors: Suzuki Noriyuki , Ishida Hatsuo 
Published: November 2002

AbstractThe miscibility between a hydrolyzed silane coupling agent, which had chemically nonreactive organofunctional groups such as propyl groups, and a film‐forming polymer [poly(vinyl acetate) PVAc] and its effect on silanol condensation were studied. A mixture consisting of a silane hydrolyzate and PVAc obtained from an alcoholic aqueous solution was investigated with Fourier transform infrared spectroscopy and size exclusion chromatography. Hydrogen bonding between the SiOH groups of the silane and the CO groups of PVAc and silanol condensation affected by PVAc were examined. The hydrogen bonding and condensation reaction were influenced by the miscibility between the organofunctional group of the silane and PVAc. The miscibility of each system was estimated from the calculated Hildebrand solubility parameter of the organofunctional group. A correlation between the miscibility and the integrated absorbance of the hydrogen‐bonded CO, obtained by least‐squares curve fitting, was established. On the basis of the molecular weight of the silane and the number of hydrogen‐bonded CO groups, a micellelike phase was proposed. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 87: 589–598, 2003
Heavy metal ion removal by amidoximated bagasse
Authors: Hassan Mohammad L. , El‐Wakil Nahla A. 
Published: November 2002

AbstractAmidoximated bagasse (Am‐B) was prepared by treating sugarcane bagasse fibers with acrylonitrile in the presence of sodium hydroxide followed by aqueous hydroxyl amine. Adsorption of some heavy metal ions, namely, Cu(II), Hg(II), Ni(II), Cr(III), and Pb(II) on the prepared Am‐B at different metal ion concentration, intervals, and temperatures was studied. Also, the selectivity of the prepared Am‐B toward adsorption of the aforementioned metal ions in an equimolar mixture was studied. The effect of regeneration of Am‐B using ethylenediamine tetraacetic acid disodium salt (EDTA), up to five times, on its adsorption capacity of the aforementioned metal ions was investigated. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 87: 666–670, 2003
Studies on essential oils, Part 33: chemical and insecticidal investigations on leaf oil of Coleus amboinicus Lour.
Authors: Singh Gurdip , Singh Om Prakash , Prasad Y. R. , de Lampasona M. P. , Catalan C. 
Published: November 2002

AbstractChemical investigations of the leaf essential oil of Coleus amboinicus by GC and GC–MS techniques indicated the presence of six components, accounting for 97% of the total oil. The major component was thymol (94.3%), followed by carvacrol (1.2%), 1,8‐cineole (0.8%), p‐cymene (0.3%), spathulenol (0.2%), terpinen‐4‐ol (0.2%) and an unidentified component (1.4%). The oil was insecticidal to white termites (Odontotermes obesus Rhamb.) with 100% mortality at a dose of 2.5 × 10−2 mg/cm3 for 5 h exposure. This oil was also more active than the synthetic insecticides, Thiodan and Primoban‐20, against termites, although it was ineffective against Tribolium castaneum, a stored product pest. Copyright © 2002 John Wiley & Sons, Ltd.
R. Radha, R. Latha and M. S. Swaminathan. 'Chemical composition and bioactivity of essential oil from Syzygium travancoricum Gamble'. Flavour and Fragrance Journal 17(5) 2002, 352–354
Authors: Radha R. , Latha R. , Swaminathan M. S. 
Published: November 2002

AbstractThe original article to which this Erratum refers was published in Flavour and Fragrance Journal 17(5) 2002, 352–354. The affiliation of R. Radha appeared incorrectly in the original paper. The correct affiliation should have appeared as follows:
R. Radha, R. Latha and M. S. Swaminathan.
M. S. Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional Area, Chennai, India 600 113.
Copyright © 2002 John Wiley & Sons, Ltd.
Carbon and oxygen isotopes: a tool for Jurassic and early Cretaceous pelagic correlation (southern Spain)
Authors: Rey J. , Delgado A. 
Published: November 2002

AbstractThe isotopic evolution of δ13C and δ18O is reported for the Jurassic and early Cretaceous in two pelagic sections of the External Zones in the Betic Cordilleras (SE Spain). Stable isotope curves from pelagic trough and swell sections display similar patterns. Variations in δ18O and δ13C values from strata at equivalent age probably reflect both early diagenetic cementation and later temperature‐related burial diagenesis.Comparison of global isotope curves with those presented in this work allows the differentiation of global from local events. Thus, the anoxic event during the early Toarcian (falciferum Zone) is characterized by elevated δ13C and depressed δ18O values. The isotopic record also allows the detection of the middle Oxfordian transgression. There are other peaks for the late Toarcian, early Bajocian, Callovian and early Berriasian that can also be used as tools for correlation purposes. Copyright © 2002 John Wiley & Sons, Ltd.
Aminophosphanes with bulky amino groups: Molecular structure, coupling constants 1J(31P, 15N) and 2J(31P, 29Si), and isotope‐induced chemical shifts 1Δ14/15N(31P)
Authors: Wrackmeyer Bernd , Köhler Christian , Milius Wolfgang , Grevy Jean Michel , García‐Hernández Zureima , Contreras Rosalinda 
Published: November 2002

AbstractThe preferred conformation of aminophosphanes with bulky amino groups (1–20) was determined by NMR spectroscopy in solution, in two cases in the solid state (11,17) and in one case (11) by X‐ray crystallography. Trimethylsilylaminodiphenylphosphanes Ph2PN(R)SiMe3 (R = Bu (1), Ph (2), 2‐pyridyl (3), 2‐pyrimidyl (4), Me3Si (5)), amino(chloro)phenylphosphanes Ph(Cl)PNRR′ (R = Bz, R′ = Me (6), R = Bz, R′ = tBu (7), R = Et, R′ = Ph (8)), amino(chloro)tert‐butylphosphanes tBu(Cl)PNRR′ (R = R′ = iPr (9), R = Me, R′ = tBu (10), R = Bz, R′ = tBu (11), R = H, R′ = tBu (12), R = Et, R′ = Ph (13), R = iPr, R′ = Ph (14), R = Bu, R′ = Ph (15), R = Bz, R′ = Ph (16), R = R′ = Ph (17), R = R′ = Me3Si (18)), 3‐tert‐butyl‐2‐chloro‐1,3,2‐oxazaphospholane (19), and benzyl(tert‐butyl)aminodichlorophosphane (20) were studied by 1H, 13C, 15N, 29Si, and 31P NMR spectroscopy. In all cases, the more bulky substituent at the nitrogen atom prefers the syn‐position with respect to the assumed orientation of the phosphorus lone pair of electrons. Many of the derivatives studied adopt this preferred conformation even at room temperature. Numerous signs of coupling constants 1J(31P, 15N), 2J(31P, 13C), and 2J(31P, 29Si) were determined. Low temperature NMR spectra were measured for derivatives for which rotation about the PN bond at room temperature is fast, showing the presence of two rotamers at low temperature. The respective conformation of these rotamers could be assigned by 13C, 15N, and 31P NMR spectroscopy. Isotope‐induced chemical shifts 1Δ15/14N(31P) were determined for all compounds at natural abundance of 15N by using Hahn‐echo extended polarization transfer experiments. The molecular structure of 11 in the solid state reveals pyramidal surroundings of the nitrogen atom and mutual trans‐positions of the tert‐butyl groups at phosphorus and nitrogen. © 2002 Wiley Periodicals, Inc. Heteroatom Chem 13:667–676, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.10084
Transcriptional activation of the tyrosine phosphatase gene, OST‐PTP, during osteoblast differentiation
Authors: Wheeler Marie A. , Townsend Melanie K. , Yunker Laurie A. , Mauro Laura J. 
Published: November 2002

AbstractProtein tyrosine phosphatases (PTPs) are critical regulators of cellular phosphorylation functioning in processes such as cell growth, differentiation, and adhesion. Osteotesticular PTP (OST) is the only characterized member of this superfamily whose expression is regulated in osteoblasts and critical for their in vitro differentiation. Such evidence would suggest that this molecule is a key modulator of signaling events during osteogenesis, yet little is known about its genetic regulation. In an effort to examine the molecular mechanisms involved in the cellular regulation of OST, we have characterized its expression in MC3T3 osteoblasts during differentiation. Northern analysis revealed that murine OST mRNA is dramatically regulated during the preosteoblast to osteoblast progression, with predominant expression in differentiated and early mineralizing osteoblasts. This expression pattern is unique to this phosphatase since, in comparison, the structurally similar receptor PTP, LAR, and the intracellular PTP1B show little change during differentiation. Cell density contributes to this upregulated expression as confluent cultures display an increase in OST transcripts within 4 h post‐plating. Transient transfection of the OST promoter in differentiating MC3T3 results in a significant increase in transcriptional activation from day 0 to day 5 of differentiation, similar in timing and intensity to the observed upregulation of the endogenous gene. This activation appears to be specific to osteoblasts, since progression to a myoblast phenotype results in no change in reporter gene activity. Culturing these preosteoblast cells in the absence of critical co‐factors results in an inhibition of differentiation and leads to a delayed induction of OST transcripts as well as the attenuation of transcriptional activation. These results show that the murine OST gene is regulated at the transcriptional level in an osteoblast‐specific, differentiation‐dependent manner during the differentiation of MC3T3 osteoblasts. Future studies will help determine the essential regulatory elements within the OST‐PTP promoter and the critical signaling pathways important in this regulation. J. Cell. Biochem. 87: 363–376, 2002. © 2002 Wiley‐Liss, Inc.
Calculated gain and threshold current density for interconduction‐subband transition in Si triple‐quantum‐well‐structures
Authors: Ghosh Sumitra , Mukhopadhyay Bratati , Basu P. K. 
Published: November 2002

AbstractWe have theoretically demonstrated population inversion between two conduction subbands in a silicon quantum well in a triple‐well structure with Si and SiGe, considering tunneling, radiative‐ and non‐radiative‐acoustic, and g‐phonon transitions. The gain spectra are then calculated and threshold current densities are estimated. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 35: 470–475, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10641
Resonant frequency and quality factors of a silver‐coated dual‐mode dielectric resonator
Authors: You Bin , Wang Xuedong , Ji Wusheng , Yang Wenrong , Li Ying 
Published: November 2002

AbstractIn this paper, resonant frequency and quality factors of a square‐corner‐cut dielectric resonator are obtained by using the finite‐difference time‐domain (FDTD) method, and the resonant frequencies of some modes in the resonator are analyzed. Some useful results are obtained. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 35: 475–477, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10642
SQP algorithms for solving Toeplitz matrix approximation problem
Authors: Al‐Homidan Suliman S. 
Published: November 2002

AbstractGiven an n × n matrix F, we find the nearest symmetric positive semi‐definite Toeplitz matrix T to F. The problem is formulated as a non‐linear minimization problem with positive semi‐definite Toeplitz matrix as constraints. Then a computational framework is given. An algorithm with rapid convergence is obtained by l1 Sequential Quadratic Programming (SQP) method. Copyright © 2002 John Wiley & Sons, Ltd.
A fast direct solver for elliptic problems with a divergence constraint
Authors: Martikainen Janne , Rossi Tuomo , Toivanen Jari 
Published: November 2002

AbstractA fast direct solution method for a discretized vector‐valued elliptic partial differential equation with a divergence constraint is considered. Such problems are typical in many disciplines such as fluid dynamics, elasticity and electromagnetics. The method requires the problem to be posed in a rectangle and boundary conditions to be either periodic boundary conditions or the so‐called slip boundary conditions in one co‐ordinate direction. The arising saddle‐point matrix has a separable form when bilinear finite elements are used in the discretization. Based on a result for so‐called p‐circulant matrices, the saddle‐point matrix can be transformed into a block‐diagonal form by fast Fourier transformations. Thus, the fast direct solver has the same structure as methods for scalar‐valued problems which are based on Fourier analysis and, therefore, it has the same computational cost 𝒪(N log N). Numerical experiments demonstrate the good efficiency and accuracy of the proposed method. Copyright © 2002 John Wiley & Sons, Ltd.
Identification of isomeric dicaffeoylquinic acids from Eleutherococcus senticosus using HPLC‐ESI/TOF/MS and 1H‐NMR methods
Authors: Tolonen Ari , Joutsamo Topi , Mattlla Sampo , Kämäräinen Terttu , Jalonen Jorma 
Published: November 2002

AbstractLiquid chromatography–electrospray time‐of‐flight mass spectrometry (HPLC‐ESI/TOF/MS) and a novel NMR technique, developed to maximise the sensitivity obtained from the standard NMR spectrometer, have been applied to the identification of the phenolic constituents of Eleutherococcus senticosus. In addition, molecular modelling and dihedral bond angle calculations based on the vicinal 3JHH‐coupling constants have been used in the unambiguous assignment of signals in the 1H‐NMR spectra. 5′‐O‐Caffeoylquinic acid and three isomeric compounds, 1′,5′‐O‐dicaffeoylquinic acid, 3′,5′‐O‐dicaffeoylquinic acid and 4′,5′‐O‐dicaffeoylquinic acid, have been isolated and identified from a sample. The isolation and structure determination of the latter two compounds are reported for the first time from this plant. Copyright © 2002 John Wiley & Sons, Ltd.
Analysis and quantitative determination of group B saponins in processed soybean products
Authors: Berhow Mark A. , Cantrell Charles L. , Duval Sandra M. , Dobbins Thomas A. , Maynes Jonathan , Vaughn Steven F. 
Published: November 2002

AbstractPure analytical standards for the major saponins present in processed soy products, the group B saponins (soyasaponins I, II, III and IV), were isolated in mg quantities by a combination of processing, precipitation/re‐solubilisation, TLC and preparative HPLC. These standards were determined to be pure by LC‐ESI/MS analysis and NMR. The standards were used to perfect a facile analytical HPLC method using spectrometric detection to determine the percent composition of the group B soyasaponins in various products from processing of soybean. Published in 2002 by John Wiley & Sons, Ltd.
Comment on “Evaluation of Two‐Center Overlap and Nuclear‐Attraction Integrals over Slater‐Type Orbitals with Integer and Noninteger Principal Quantum Numbers”
Authors: Guseinov I. I. 
Published: November 2002

AbstractRecently published formulas for the evaluation of two‐center overlap and nuclear‐attraction integrals (Özdogan, T.; Orbay, M. Int J Quantum Chem 2002, 87, 15) are critically analyzed. It is proved that the general formula presented in this work for the expansion of the product of two normalized associated Legendre functions in ellipsoidal coordinates is obtained from the expansion relationships contained in our articles (Guseinov, I. I. J Phys B 1970, 3, 1399; Phys Rev A 1985, 32, 1864; J Mol Struct THEOCHEM 1995, 336, 17) by changing the summation indices. It is demonstrated that the formulas published by these authors results in two‐center overlap and nuclear‐attraction integrals that are also derived from our formulas. © 2002 Wiley Periodicals, Inc. Int J Quantum Chem, 2003
Energy‐linearized variational cellular method for large molecules and solids
Authors: Nesbet R. K. 
Published: November 2002

AbstractMultiple scattering theory (MST) is the mainstay of Kohn–Sham calculations of the electronic structure of solids and alloys. MST formalism solves one‐electron equations within each atomic cell, using a Green function to propagate solutions across cell boundaries, while standard methodology for molecules expands wave functions in a Gaussian orbital basis. An energy‐linearized version of MST (LMTO) is efficient but restricted to an atomic‐sphere model. Full‐potential MST extends the formalism to space‐filling Wigner–Seitz polyhedra. The variational cellular method (VCM) solves full‐potential equations, replacing Green‐function propagation (structure constants) by variational matching at the interfaces of adjacent atomic cells. VCM provides a common formalism for molecules and solids but cannot easily be converted to an energy‐linearized method. A new variational principle is derived here that extends the VCM to a straightforward procedure for energy linearization. This formalism eliminates false solutions from the VCM. © 2002 Wiley Periodicals, Inc. Int J Quantum Chem, 2003