bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Interprofessional Interactive Case-Based Discussion Session for Healthcare Professions Students
Authors: Prasad Samiksha , O’Malley Chasity , Tailor Marylee , Rajput Vijay , Fine Lauren 
Published: January 2024

Abstract
BACKGROUND: Interprofessional education (IPE) prepares future health professional learners for IP collaboration and teamwork and can navigate the existing organizational culture, enhance patient safety, reduce errors, and improve the quality of care for patients through collaborative care and practice.
METHODS AND FINDINGS: This study of IPE sessions with students from 10 healthcare programs was conducted during a virtual IPE day across three consecutive years (2021-23). The session involved active learning through a small-group problem solving session with a five-point Likert scale pre- and post-survey instruments. The change in student perceptions of interprofessional education and comfort with working with other healthcare professionals was assessed with 62.96% of health professions learners attending the sessions between 2021-2023. Learners (87.5%) agreed that the session strengthened their stance on practicing an approach to discuss treatment plans with other healthcare providers. Qualitative analysis reported emphasis on communication (28%) and collaboration (18%) as key threads.
CONCLUSION: Learners recognized the importance of communication in IPE settings for teamwork and collaboration for continuity of care and patient safety. The virtual methods of the session enabled participants from different geographical campuses to engage in the IPE.
Ethnobotany of traditional cosmetics among the Oromo Women in Madda Walabu District, Bale Zone, Southeastern Ethiopia
Authors: Sultan Siraj , Telila Habte , Kumsa Lemessa 
Published: January 2024

Abstract
The use of plants as natural cosmetics has been perceived as a source of welfare. However, the plants and the associated indigenous knowledge are continuously depleting globally. The study thus aimed to investigate the ethnobotany of traditional cosmetics among Oromo women in the Madda Walabu District of Bale Zone, Southeastern Oromia, Ethiopia. Ethnobotanical information such as plant species used for traditional cosmetics, method of preparation, and administration were collected among 150 Oromo women using semi-structured questionnaires. Thereafter, quantitative ethnobotanical indices, including the Informant consensus factor (ICF), and the level of fidelity (Fl) were computed to determine the most important species used by the women. A total of 48 plant species belonging to 31 families used as sources of traditional cosmetics were recorded among Oromo women in the district. The most frequent family was Fabaceae followed by Bigonaceae, Burseraceae, and Combretaceae. Most of the plants used as traditional cosmetics were trees, followed by shrubs and herbs in life forms Leaves are the most commonly used plant part in traditional cosmetics followed by barks; and maceration and decoction are the most common preparation methods applied to prepare plant-based traditional cosmetics. Traditional cosmetics are applied to various parts of the body and are mainly administered topically while oral infusions were not common. All categories had high ICF values, ranging from 0.80 to 0.98. The highest ICF value was found for skin treatment, followed by the hair, and Face mask. Overall, Commiphora habessinica,Gnidia stenophylla, Kalanchoe lanceolata, Mimusops kummel, Sesamothamnus rivae, Terminalia brownii, T. laxiflora, Acacia drepanolobium, A. mellifera and Aloe citrina were the most frequently cited plants by Oromo women for traditional cosmetic purposes in the study area. However, there will be a need to conduct laboratory-based experiments to establish the efficacy and safety of these documented plants using relevant biological assays.
Four weeks of off-treatment follow-up is sufficient to determine virologic responses at off-treatment week 12 in patients with hepatitis C virus infection receiving pangenotypic direct-acting antivirals
Authors: Liu Chen-Hua , Chang Yu-Ping , Lee Ji-Yuh , Chen Chi-Yi , Kao Wei-Yu , Lin Chih-Lin , Yang Sheng-Shun , Shih Yu-Lueng , Peng Cheng-Yuan , Lee Fu-Jen , Tsai Ming-Chang , Huang Shang-Chin , Su Tung-Hung , Tseng Tai-Chung , Liu Chun-Jen , Chen Pei-Jer , Kao Jia-Horng 
Published: January 2024

Abstract

Background: Early confirmation of sustained virologic response (SVR) or viral relapse after direct-acting antivirals (DAAs) for hepatitis C virus (HCV) infection is essential based on public health perspectives, particularly for patients with high risk of nonadherence to posttreatment follow-ups.
Methods: A total of 1011 patients who achieved end-of-treatment virologic response, including 526 receiving pangenotypic DAAs, and 485 receiving genotype-specific DAAs, who had available off-treatment weeks 4 and 12 serum HCV RNA data to confirm SVR at off-treatment week 12 (SVR12) or viral relapse were included in the analysis. The positive predictive value (PPV) and negative predictive value (NPV) of SVR4 to predict patients with SVR12 or viral relapse were reported. Furthermore, we analyzed the concordance rate between SVR12 and SVR24 in 943 patients with available SVR24 data.
Results: The PPV and NPV of SVR4 to predict SVR12 were 98.5% (95% confidence interval [CI]: 98.0%-98.9%) and 100% (95% CI: 66.4%-100%) in the entire population. The PPV of SVR4 to predict SVR12 in patients receiving pangenotypic DAAs was higher than those receiving genotype-specific DAAs (99.8% [95% CI: 98.9%-100%] versus 97.1% [95% CI: 96.2%-97.8%], p < 0.001). The NPVs of SVR4 to predict viral relapse were 100%, regardless of type of DAAs. Moreover, the concordance rate between SVR12 and SVR24 was 100%.
Conclusion: In patients with HCV who are treated with pangenotypic DAAs, an off-treatment week 4 serum HCV RNA testing is sufficient to provide an excellent prediction power of SVR or viral relapse at off-treatment week 12.
The new phthalic acid-based DES as a versatile catalyst for the synthesis of pyrimido[4,5-d]pyrimidines and pyrano[3,2-c]chromenes
Authors: Monem Arezo , Habibi Davood , Alizadeh Abdolhamid , Goudarzi Hadis 
Published: January 2024

Abstract
Deep eutectic solvents (DESs) have found many applications in recent years and with countless advantages over regular solvents, not only complying with green chemistry principles but also acting as catalysts depending on their properties. In this regard, a new DES (MTPPBr-PHTH-DES) was prepared from a mixture (molar ratio 1:1) of methyltriphenylphosphonium bromide (MTPPBr) and phthalic acid (PHTH), characterized with various techniques such as FT-IR, TGA/DTA, densitometer, eutectic point, and 1H NMR and used as an acid catalyst in the synthesis of pyrimido[4,5-d]pyrimidines and pyrano[3,2-c]chromes in solvent-free conditions. Short reaction time, high efficiency, and easy recycling and separation of the DES catalyst are among the most important features of the presented method.
Dental pulp mesenchymal stem cell-derived exosomes inhibit neuroinflammation and microglial pyroptosis in subarachnoid hemorrhage via the miRNA-197-3p/Foxo3 axis
Authors: Liang Xin , Miao Yan , Tong Xin , Chen Jigang , Liu Hongyi , He Zilong , Liu Aihua , Hu Zhiqiang 
Published: January 2024

Abstract

Background
Subarachnoid hemorrhage (SAH) is a severe stroke subtype that lacks effective treatment. Exosomes derived from human dental pulp stem cells (DPSCs) are a promising acellular therapeutic strategy for neurological diseases. However, the therapeutic effects of DPSC-derived exosomes (DPSC-Exos) on SAH remain unknown. In this study, we investigated the therapeutic effects and mechanisms of action of DPSC-Exos in SAH.
Materials and Methods
SAH was established using 120 male Sprague-Dawley rats. One hour after SAH induction, DPSC-Exos were administered via tail vein injection. To investigate the effect of DPSC-Exos, SAH grading, short-term and long-term neurobehavioral assessments, brain water content, western blot (WB), immunofluorescence staining, Nissl staining, and HE staining were performed. The role of miR-197-3p/FOXO3 in regulating pyroptosis was demonstrated through miRNA sequencing, bioinformatics analysis, and rescue experiments. The SAH model in vitro was established by stimulating BV2 cells with hemoglobin (Hb) and the underlying mechanism of DPSC-Exos was investigated through WB and Hoechst/PI staining.
Results
The expressions of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were increased after SAH. DPSC-Exos alleviated brain edema and neuroinflammation by inhibiting the expression of FOXO3 and reducing NLRP3 inflammasome activation, leading to improved neurobehavioral functions at 24 h after SAH. In vitro, the expression of the NLRP3 inflammasome components (NLRP3 and caspase1-p20), GSDMD-N, and IL-18 was inhibited in BV2 cells pretreated with DPSC-Exos. Importantly, DPSC-Exos overexpressing miR-197-3p had a more obvious protective effect than those from NC-transfected DPSCs, while those from DPSCs transfected with the miR-197-3p inhibitor had a weaker protective effect. Functional studies indicated that miR-197-3p bound to the 3ʹ-untranslated region of FOXO3, inhibiting its transcription. Furthermore, the overexpression of FOXO3 reversed the protective effects of miR-197-3p.
Conclusions
DPSC-Exos inhibited activation of the NLRP3 inflammasome and related cytokine release via the miR-197-3p/FOXO3 pathway, alleviated neuroinflammation, and inhibited microglial pyroptosis. These findings suggest that using DPSC-Exos is a promising therapeutic strategy for SAH. .
Corneal Transplant Awareness in Medical Students by Grade and the Effect of Subject- Specific Education on This Awareness Level
Authors: Ayas Atanur , Kaplan Anil , Bilen Alperen , Colak Aysegul , Köse Timur , Selver Ozlem Barut 
Published: January 2024

Abstract

Purpose
Corneal transplantation is the most commonly performed transplant surgery. Medical students, doctors of the future, will educate the public about organ transplantation and raise public awareness on this issue. The first aim of this study is to evaluate the initial awareness of medical students, about corneal transplantation, then to provide training to these students and finally to evaluate the change in their knowledge on this subject.
Method
310 medical students from Ege University were asked to participate in a 20-question survey. Later, they were given education by a corneal transplant specialist. Immediately after the lessons, the survey was reapplied.
Results
The number of students who said they had knowledge about corneal transplantation before the training was 25.5% (n = 79). The knowledge level of 1st grades was lower than the 5th and 6th grades. Final-test scores were significantly higher than pre-test scores in each year group (p < 0.001). The final-test scores were significantly higher than the pre-test scores in both groups, who said they had knowledge about corneal transplantation and who said they did not.
Conclusion
Physicians' approach to organ transplantation can significantly determine the level of interest in organ donation and physicians’ level of knowledge about organ transplantation determine their behaviors as well. This article showed the level of knowledge about corneal transplantation does not increase linearly as the medical school year progresses. In addition, regardless of the initial level of knowledge, the education on corneal transplantation will increase the knowledge level of every student from the 1st grade to the 6th grade.
High implantation of coronary arteries after the Switch procedure and associated stenoses
Authors: Al-Kassmy Jawad , Hadid Mehdi , Dib Nabil , Poirier Nancy , Miro Joaquim , Dahdah Nagib 
Published: January 2024

Abstract

Background: Surgical repair through the Arterial Switch Operation (ASO) is the only definitive treatment in patients with Dextro-Transposition of the Great Arteries (d-TGA). A crucial step during the implantation process is transfer of coronary arteries (CA) to the neo-aorta. A potential cause of CA stenosis is the presence of a high take off CA, defined by the presence of coronary ostium located above the sinotubular junction (STJ) of the aorta.
Methods: We conducted a retrospective study on 82 patients with d-TGA between 2010 and 2018 in a tertiary paediatric hospital in Canada.
Results: Fifty six-patients (68%) had at least one of the CA at a high take-off implantation above the STJ. The mean distance from the STJ was +7.12 ± 3.38 mm for the RCA, and +6.82 ± 3.05 mm for the LMCA. Out of the 56 patients with highly implanted CA, 4 patients (7%) had stenosis, and out of 26 patients with in-sinus implanted CA, one patient (4%) had stenosis (p = 0.16). Patients in the HICA group with stenosis had a lower height of implantation of the CA compared to those without stenosis (+4.50 ± 1.32 mm vs. + 6.82 ± 3.05 mm, respectively; p < 0.05).
Conclusion: This is a rare study assessing the rates of stenosis in the context of in-sinus versus high CA implantation in the ASO. Implanting the coronary ostia at a higher level than the expected natural level does not seem to be associated with a significant risk in compromising CA perfusion.
Free radical polymerization facilitated covalent conjugation enables anchoring-omitting expansion microscopy
Authors: He Zhi-Ke , Yi Kebing , Zhang Yaran , Zhou Xinyue , Liu Yanchao , Du Chuanxin , Tinnefeld Philip , Wang Fubing , Zhou Fuxiang , Ji Xinghu 
Published: January 2024

Abstract
Expansion microscopy (ExM) enables nanoscale imaging on diffraction-limited fluorescent microscopes. However, it still remains elusive how biological samples can be covalently attached to hydrogels even without the addition of anchoring chemicals. As a newly developed technique, elucidating the underlying anchoring chemistries in ExM would be informative for its rational design and future development. Here, we present a free radical polymerization facilitated covalent conjugation (FRAPACON) strategy that enables the direct linkage between proteins and polymers without the addition of custom-synthesized tags or bivalent anchors. This modification-free strategy is compatible with a wide range of proteins and monomers, and also allows the polymerization on the yeast cell surface. The intrinsic biological functions of proteins are well-preserved after FRAPACON reaction. Further, we implement ExM on brain slices with satisfactory fluorescence retention and distortion performance in an anchoring-omitting manner. This work demonstrates the anchoring chemistries for protein access in ExM and possibly provides a new perspective for rapid labeling of biomolecules.
Practice list size, workforce composition and performance in English General Practice: A Latent Profile Analysis
Authors: Kayira Alfred Bornwell , Painter Helena , Mathur Rohini , Ford John 
Published: January 2024

Abstract

Background

General practices in England have grown in size from traditionally small, General Practitioner (GP) led practices to large multidisciplinary enterprises. We assessed the effect of practice list size and workforce composition on practice performance in clinical outcomes and patient experience.

Methods

We linked five English practice-level datasets relating to workforce, list size, age, deprivation, rurality, patient experience and Quality and Outcomes Framework (QOF) and non-QOF clinical processes and outcomes. Latent Profile Analysis (LPA) was used to cluster general practices into groups based on list size and workforce composition. Bayesian Information Criterion, Akaike Information Criterion and deliberation within the research team were used to determine the most informative number of groups. One-way ANOVA was used to assess how groups differed on indicator variables and other variables of interest. Linear regression was used to assess the association between practice group and practice performance.

Results

A total of 6024 practices were available for class assignment. We determined that a 3-class grouping provided the most meaningful interpretation; 4494 (74.6%) were classified as ‘Small GP-reliant practices’, 1400 (23.2%) were labelled ‘Medium-size GP-led practices with a multidisciplinary team (MDT) input’ and 131 (2.2%) practices were named ‘Large multidisciplinary practices’. Small GP-reliant practices outperformed larger multidisciplinary practices on all patient-reported indicators except on confidence and trust where medium-size GP-led practices with MDT input had favourable ratings. There was no difference in performance between small GP-reliant practices and larger multidisciplinary practices on QOF incentivised indicators except on asthma reviews where medium-size GP-led practices with MDT input performed worse than smaller GP-reliant practices and immunisation coverage where the same group performed better than smaller GP-reliant practices. For non-incentivised indicators, larger multidisciplinary practices had higher cancer detection rates than smaller GP-reliant practices.

Conclusion

Small GP-reliant practices were found to provide better access, continuity, patient experience and satisfaction with care. Larger multidisciplinary practices had higher cancer detection rates but no effect on other clinical processes and outcomes. As England moves towards larger multidisciplinary practices efforts should be made to preserve high-quality patient experience.
Impact Of Attaching Intermediate Vertical Member (IVM) On Long Span Special Truss Moment Frame (STMF) Performance
Authors: Pradipta Bramanta Fajar , Kusumastuti Dyah 
Published: January 2024

Abstract
One of the seismic load resisting systems in steel structure building is the Special Truss Moment Frame (STMF). One of the advantages of STMF that is highlighted is that the STMF structure is suitable for use in structures with long spans portal. As for the governing code, it is stipulated that the maximum span limit that may be used in STMF structure is 20 m. This limitation is regulated because STMF structure with > 20 m bay shows poor performance. This issue will be tried to be resolved by attaching an Intermediate Vertical Member (IVM), therefore STMF structure can be utilized for > 20 m bay. The structure was evaluated using the Nonlinear Static Pushover methods. The results of the analysis are used to evaluate the effect of attaching IVM components to the STMF structure by it’s entire performance. The analysis results show that the attachment of IVM to the STMF structure results in improved performance such as strength, ductility and the acceptance criteria of the structure globally.
Crafting a High-Fidelity Mobile Application to Mitigate Death Anxiety Amidst the COVID-19 Pandemic
Authors: Shakeri Aliakbar , Saeidnia Hamid Reza , Keshavarz Hamid , Babajani Afshin , Mohammadzadeh Zahra , Hassanzadeh Mohammad 
Published: January 2024

Abstract

Background: The COVID-19 pandemic had a profound impact on individuals, organizations, and society as a whole. One of the consequences of this crisis was an increase in death anxiety among both the general population and patients. Anxiety disorders, including death anxiety, can be effectively treated using exposure therapy, which is a well-established method. To further support medical practitioners, it would be beneficial to develop mobile-based applications and content that focus on this treatment approach. With this in mind, the objective of the present study was to develop a prototype for a proposed mobile application aimed at alleviating the burden of COVID-19 death anxiety.
Methods: Our research adopts a structured approach grounded in the five essential phases of high-fidelity prototype design. These methodological steps are as follows: 1) Goal Definition, involving meticulous planning and explicit delineation of the primary purpose, alongside the identification of materials utilized in the prototype. 2) User Interface Design, entailing the creation of diverse interface designs to discern and select the optimal design. 3) Adding Interactions, encompassing the incorporation of interactive elements such as clicking, dragging, scrolling, and user input into the prototype. 4) Testing and Evaluation, comprising prototype evaluation and systematic feedback collection. 5) Iteration and Improvement, where the prototype undergoes refinement based on the conclusive feedback garnered during the evaluation phase, aiming to attain the desired prototype. This methodological framework ensures a comprehensive and systematic approach to the development and enhancement of our high-fidelity prototype.
Result: After conducting interviews, it was found that certain subjects such as near-death experiences, habit control, death imagery, self-confidence, and anxiety management were identified as the most crucial criteria to be included in the mobile application. Furthermore, feedback from online workshops emphasized the importance of ensuring that the application is easy to use and provides useful features. Taking all these factors into consideration, a prototype was developed using the Figma software, employing a high-fidelity technique to create an initial version of the mobile application.
Conclusions: In the current challenging time of the COVID-19 pandemic, the identified criteria for content production and the proposed prototype serve as valuable resources for software designers aiming to design and develop suitable applications to alleviate anxiety related to COVID-19 death. By following these criteria and utilizing the prototype as a guide, software designers can create applications that effectively address the anxieties and concerns of individuals during this difficult period.
Development and Validation of a Prognostic Model for Short-Term Outcomes in NDM- MAC
Authors: Gao Shuohui , Tian Shengqi , Xing Jianpeng , Zhou Xuanpeng , Song Xinyuan 
Published: January 2024

Abstract
Purpose: The objective of this study was to develop a nomogram that can be used to predict the 3-year overall survival (OS) of patients with non-distant metastatic mucinous adenocarcinoma of the colon (NDM-MAC).
Methods:From the SEER (Surveillance, Epidemiology, and End Results) database, a total of 1675 patients with NDM-MAC were extracted and utilized as a training cohort. A multivariate COX regression model was developed and visualized as a nomogram in the training cohort. Furthermore, an additional 207 patients were enlisted from the China-Japan Union Hospital, Jilin University, to constitute an external validation cohort. Decision curve analysis (DCA) curves, C-index, AUC values, and internal and external validation calibration curves were used to assess the established model. The training cohort underwent retrospective scoring using a nomogram. The X-Tile software was utilized to determine the two optimal cutoff values, to develop a risk assessment model.
Results:Age, T stage, N stage, Tumor site, and the quantity of harvested lymph nodes (Harvested LN) were included as five variables influencing the 3-year OS to construct the nomogram. The calibration curves of the nomogram demonstrate a good consistency between the predicted OS and the actual OS. The C-index for both internal validation and external validation was 0.703 (P < 0.001) and 0.699 (P < 0.001). NDM-MAC patients were divided into three categories according to the risk assessment model. The survival statistics showed a substantial difference (P<0.001) between the subgroups.
Conclusion:In conclusion, this study developed a nomogram for NDM-MAC that included 5 variables.