bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conservative management of a fracture of the greater trochanter of the femur in an adult horse
Authors: Peeters Manon W. J. , Wallace Andrew G. , Chesworth Matthew J. , Berner Dagmar , Fiske‐Jackson Andrew R. 
Published: February 2024

SummaryAn 8‐year‐old Warmblood cross mare was presented for further investigation of an acute‐onset lameness of the right hindlimb. Three weeks prior to presentation, the horse was found markedly lame at walk following a fall during exercise. The horse was initially managed with field rest, but the lameness persisted. A marked right hindlimb lameness at trot graded 5/10 was confirmed. Atrophy of the right gluteal musculature was evident, and palpation over the region of the right coxofemoral joint provoked a pain reaction. Nuclear scintigraphy revealed a diffuse area of increased radiopharmaceutical uptake in the right femoral head, neck and greater trochanter. Radiographs identified a complete, slightly displaced fracture of the base of the greater trochanter of the right femur. Ultrasound examination of the proximal femur demonstrated an irregularity of the caudolateral bone surface of the greater trochanter, extending along its attachment to the femur; this was consistent with the radiological findings. On re‐examination, after 9 months of stable rest, the horse was found to be sound when trotted in a straight line on the hard surface. Follow‐up two and a half years after the initial incident reported the horse to be nonlame and at its previous level of work.
Domestic or export: What is basic at the NUTS 2 regional level? A spatial endogenous regional growth model applied in the EU
Authors: Ricordel Pascal 
Published: February 2024

AbstractA common statement found in regional policy reports is that regional growth is an “export or die” issue. However, the succession of disruptions in the international supply chain has highlighted the crucial roles of domestic activities, local markets and short supply chains, turning the environmental and resilience challenge present in growth policy into a “domestic or die” issue. Recent regional growth theories have seriously questioned export activity as the only way in which to drive regional growth and have highlighted the crucial role of the domestic sector. However, no empirical study has assessed the roles of the domestic and export sectors in growth during this troubled economic period, despite the usefulness of this information for nonbiased policy decisions. Using a spatial endogenous regional growth model as a framework, we investigate the role of the domestic sector during the 1999–2014 period for 263 European Union (EU) Nomenclature of Territorial Units for Statistics (NUTS) regions. The results stress the importance of domestic productivity for regional growth during this period, which is characterized by three economic shocks, thereby elucidating the importance of domestic productivity for competitiveness and resilience issues.
Whole blood clotting time: Current practices among Thailand National External Quality Assessment Scheme for blood coagulation member laboratories
Authors: Wongkrajang Preechaya , Chinswangwatanakul Wimol , Tientadakul Panutsaya , Chantarat Achara 
Published: February 2024

AbstractIntroductionThe Thai National Guidelines for Hemostatic Laboratory Testing were established in 2018. The guidelines recommend that the 20‐min whole blood clotting time (20WBCT) method be used to diagnose/monitor snake bites. The aim of this study was to survey members of the Thailand National External Quality Assessment Scheme (NEQAS) for Blood Coagulation to investigate the use of 20WBCT testing compared between the 2021 post‐guideline and 2007 pre‐guideline periods.MethodsIn July 2021, questionnaires were sent from the Faculty of Medicine Siriraj Hospital, Mahidol University to 521 Thailand NEQAS for Blood Coagulation member laboratories to survey their WBCT practices. Current WBCT practices were compared with pre‐guideline WBCT practices, and chi‐square test (x2) was used to test for differences between groups.ResultsNinety‐seven (18.6%) of 521 surveys were returned. Seventy‐one laboratories (73.2%) reported knowing about 20WBCT from the Thai national guidelines. The reported average frequency of overall WBCT testing in 2021 was 12.4 times/month. The proportion of laboratories that reported using the 20WBCT test increased from 2.0% in 2007 to 46.4% in 2021 (p < 0.001), and the indications for performing WBCT were virtually unchanged from 2007 to 2021. The proportion of laboratories that reported having problems with WBCT testing decreased from 32.7% in 2007 to 16.5% in 2021.ConclusionDespite our findings that almost three‐quarters of respondent laboratories reported knowing about 20WBCT testing from the WBCT guidelines, and that WBCT‐specific problems decreased significantly from 2007 to 2021, more work and training is needed to improve WBCT guideline dissemination, understanding, and adherence in Thailand.
The effect of oscillation depolymerization on ethylenediaminetetraacetic acid‐dependent platelet aggregation samples: A cross‐over study
Authors: Liu Dan , Yang Wei 
Published: February 2024

AbstractIntroductionEthylenediaminetetraacetic acid (EDTA)‐dependent platelet aggregation (PA) can cause medical errors. Currently, there is no reliable method for completely solving this problem. This study aims to solve this problem that has plagued clinical practice for many years by using oscillation method.MethodsSixty‐one EDTA‐PA samples were collected, divided, and disaggregated using the oscillation method at various times and speeds. The samples were analyzed using routine blood tests and blood smears.ResultsPlatelet counts (PLT) were increased significantly after oscillation. PLT in the 3000 rpm for 0.5 min group was significantly higher than that in the 500 rpm for 0.5 min group (p < 0. 01). After 3000 rpm oscillation, the PLT gradually increased with time, while compared with the 10‐min group, the PLT in the 13‐min group showed no significant differences. The effective disaggregation rates in the EDTA‐PA samples using the oscillation method and sodium citrate anticoagulant were 96.72% and 65.57%, respectively. There were no significant changes in white blood cell (WBC) or red blood cell (RBC) counts or morphology after the use of the oscillation method.ConclusionThe oscillation method effectively depolymerized EDTA‐PA without adverse effects on WBC and RBC. The implementation of this technique promises to resolve the issue of EDTA‐PA.
Intimate partner violence in the lives of Indigenous and Black women in the upper Midwest of the United States during the COVID‐19 pandemic: A mixed‐methods protocol examining help‐seeking behaviours and experiences
Authors: Lopez Alexa A. , Dressel Anne , Luebke Jeneile , Williams Joni , Campbell Jennifer , Miller Jessica , Kibicho Jennifer , Schadewald Diane , Abusbaitan Hanan , Pirsch Anna , Gondwe Kaboni W. , Schubert Erin , Ruiz Ashley , Kako Peninnah , Mkandawire‐Valhmu Lucy , Egede Leonard E. 
Published: February 2024

AbstractViolent behaviour perpetrated against women has long‐lasting negative physical and mental health consequences for women, their children, their families, and their communities. Intimate partner violence (IPV) is associated with many adverse physical, psychological, and emotional consequences. Structural racism and historical trauma affect women's trust and further hinder the ability of Indigenous and Black women to seek help after experiencing IPV. The availability of IPV support services, which can include shelter, food, group therapy, legal assistance, and advocacy, can be inaccessible to women due to the inability to access often limited resources in urban environments and reasons compounded by potential geographic distance if living in rural areas or living in community. Understanding the unique reasons why Indigenous and Black women do not seek help, and the barriers they experience when seeking help after IPV, is critical. Pandemics have the potential to create further complexities on how IPV is experienced. Black and Indigenous women experiencing IPV were therefore at even greater risk for IPV‐related harm because of state and local “stay at home” measures put in place to minimise the spread COVID‐19. The purpose of this manuscript is to explicate the methods for a large R01 study in the Upper Midwest.
Efficacy of autologous platelet‐rich plasma combined with negative pressure sealing drainage in the treatment of patients with diabetic foot ulcer
Authors: Liao Ming‐Yan , Ran Shu‐Rong 
Published: February 2024

AbstractThis study systematically evaluates the efficacy of autologous platelet‐rich plasma (PRP) combined with negative pressure sealing drainage (NPSD) in the treatment of diabetic foot ulcers (DFU). A comprehensive computerized search was conducted in PubMed, Embase, Google Scholar, Cochrane Library, China National Knowledge Infrastructure, and Wanfang databases for randomized controlled trials (RCTs), from database inception to November 2023, on the treatment of DFU using autologous PRP combined with NPSD. Two researchers independently screened the literature, extracted data, and conducted quality assessments based on inclusion and exclusion criteria. Data analysis was performed using RevMan 5.4 software. A total of 17 RCTs involving 1325 DFU patients were included. The analysis revealed that the application of autologous PRP combined with NPSD in DFU patients significantly increased the ulcer healing rate (OR = 3.54, 95% CI: 2.71–4.62, p < 0.001) and the overall treatment efficacy rate (OR = 4.99, 95% CI: 3.46–7.19, p < 0.001), and significantly reduced the wound healing time (SMD = −2.05, 95% CI: −2.61 to −1.49, p < 0.001). This study indicates that the treatment of DFU with autologous PRP combined with NPSD accelerates wound healing, improves the overall treatment efficacy rate, and offers a new option for the management of DFU.
Comparative phylogeography shows congruent co‐divergence in Neotropical seasonally dry forest and savanna tree species
Authors: Vitorino Luciana Cristina , Reis Mateus Neri Oliveira , Melo Warita Alves , Collevatti Rosane G. 
Published: February 2024

AbstractAimThe response of South American seasonally dry tropical forest (SDTF) and savanna species to the Quaternary climate changes is still poorly understood. Here we use multi‐model inference approach to compare the phylogeography and demographic history of five trumpet tree species (also known as pau‐d'arco) and draw general biogeographical patterns: Handroanthus impetiginosus, H. ochraceus, H. serratifolius, Tabebuia aurea and T. roseoalba.LocationAmazonia, Atlantic Forest, Cerrado and Caatinga ecoregions, South America and Brazil.TaxaBignoniaceae, Tabebuia Alliance, trees.MethodsWe compiled genetic data, occurrence records and ecological niche modelling (ENM) from published articles. We used coalescent modelling to test patterns of co‐divergence, and analysed niche breadth and overlap and spatial patterns in genetic diversity using quantile regressions.ResultsSpecies showed concordant lineage divergence times in the Pleistocene. Lineage diversification and phylogeographical patterns are more related to species niche breadth than to biome (savanna or SDTF). Differences in genetic diversity among species matches the expected for the demographic scenarios obtained with coalescent simulations and ENMs: species with wider range expansion during the Last Glacial Maximum had higher diversity than species with range retraction or multiple refugia.Main ConclusionsDiversification burst in the Pliocene, and major diversification of lineages during the Pleistocene seems to be a general pattern in SDTF and savannas in the South America. The high number of haplotypes at the Central Brazil, corresponding to the Cerrado ecoregion, highlights the importance of Protected Areas for SDTFs and savannas, highly threatened biomes in Brazil.
Effects of allisartan‐isoproxil‐based combination antihypertensive regimen in hypertensive patients with microalbuminuria or hyperuricemia
Authors: Sun Ningling , Wang Hongyi 
Published: February 2024

AbstractMicroalbuminuria and hyperuricemia management are crucial for the integrated management of hypertensive patients. This retrospective post hoc analysis aims to evaluate the optimal allisartan‐isoproxil‐based combination regimen for hypertensive patients with microalbuminuria or hyperuricemia. A total of 460 hypertensive patients with microalbuminuria and 486 hypertensive patients with hyperuricemia were included in this study. All patients were initially treated with allisartan‐isoproxil for 4 weeks. Thereafter, patients with blood pressure (BP) < 140/90 mmHg continued the monotherapy for 8 weeks; patients with BP ≥140/90 mmHg were randomly assigned in a 1:1 ratio to receive allisartan‐isoproxil + amlodipine (Group A + C) or allisartan‐isoproxil + indapamide (Group A + D) for 8 weeks. The changes of BP, urinary albumin and serum uric acid (UA) were measured. In patients with microalbuminuria, the urinary albumin/creatinine ratio (UACR) significantly decreased by 10.4 mg/g in Group A + C (vs. baseline p = .0035) and 24.2 mg/g in Group A + D (vs baseline p < .0001), intergroup p = NS. In patients with hyperuricemia, serum UA level decreased by 44.5 µmol/L in Group A + C (vs. baseline p = .0003), but increased by 27.2 µmol/L in Group A + D (vs. baseline p = .0167), intergroup p < .0001. The results suggest that for hypertensive patients with microalbuminuria, angiotensin receptor blocker (ARB) + calcium channel blocker (CCB) or ARB+ diuretic both are good choices based on their improvement of microalbuminuria and BP. But for patients with hyperuricemia, ARB + diuretic may further increase the level of UA.
The novel TERF2::PDGFRB fusion gene enhances tumorigenesis via PDGFRB/STAT5 signalling pathways and sensitivity to TKI in ph‐like ALL
Authors: Xu Guo‐fa , Zeng Zhao , Zhang Zhi‐bo , Zhang Xiao‐mei , Wang Man , Xiao Qing , Li Jun , Xie Xiao‐qing , He Sanxiu , Fu Hui‐hui , Liu Yi , Yang Zai‐liang , Chen Yu , Shi Jie , Wang Biao , Qiu Hui‐ying , Zhou Qi , Liu Yao , Chen Su‐ning 
Published: February 2024

AbstractPatients with Philadelphia chromosome‐like acute lymphoblastic leukaemia (Ph‐like ALL) often face a grim prognosis, with PDGFRB gene fusions being commonly detected in this subgroup. Our study has unveiled a newfound fusion gene, TERF2::PDGFRB, and we have found that patients carrying this fusion gene exhibit sensitivity to dasatinib. Ba/F3 cells harbouring the TERF2::PDGFRB fusion display IL‐3‐independent cell proliferation through activation of the p‐PDGFRB and p‐STAT5 signalling pathways. These cells exhibit reduced apoptosis and demonstrate sensitivity to imatinib in vitro. When transfused into mice, Ba/F3 cells with the TERF2::PDGFRB fusion gene induce tumorigenesis and a shortened lifespan in cell‐derived graft models, but this outcome can be improved with imatinib treatment. In summary, we have identified the novel TERF2::PDGFRB fusion gene, which exhibits oncogenic potential both in vitro and in vivo, making it a potential therapeutic target for tyrosine kinase inhibitors (TKIs).
“They study for six years. We study for generations”: Renegotiating birth, power, and interculturalidad in the Ecuadorian Amazon
Authors: Reichert Alexandra J. 
Published: February 2024

AbstractThis article explores how kichwa midwives negotiate interculturality as both cultural knowledge‐holders and clinical practitioners. Kichwa midwives in the Ecuadorian Amazon face a dynamic set of barriers in their position as healers, birth care givers, and indigenous activists, including intercultural government policies aimed to delegitimize them, consistent othering in relationship to biomedicine, and shifting generational involvement in kichwa health practices. Midwives engage in a complex set of relationships with the state, relying on it for the “rights” to practice their knowledge, while simultaneously being undermined by the state's promise of interculturality. I demonstrate how kichwa midwives practice “strategic entanglement” as a form of resistance and argue for a renegotiation of the definition and role of interculturalidad in the context of state‐controlled birth care.
Microdosing ketamine in Drosophila does not block serotonin reuptake, but causes complex behavioral changes mediated by glutamate and serotonin receptors
Authors: Dunham Kelly E. , Khaled Kani H. , Weizman Leah , Venton B. Jill 
Published: February 2024

AbstractMicrodosing ketamine is a novel antidepressant for treatment‐resistant depression. Traditional antidepressants, like selective serotonin reuptake inhibitors (SSRIs), inhibit serotonin reuptake, but it is not clear if ketamine shows a similar mechanism. Here, we tested the effects of feeding ketamine and SSRIs to Drosophila melanogaster larvae, which has a similar serotonin system to mammals and is a good model to track depressive behaviors, such as locomotion and feeding. Fast‐scan cyclic voltammetry (FSCV) was used to measure optogenetically stimulated serotonin changes, and locomotion tracking software and blue dye feeding to monitor behavior. We fed larvae various doses (1–100 mM) of antidepressants for 24 h and found that 1 mM ketamine did not affect serotonin, but increased locomotion and feeding. Low doses (≤10 mM) of escitalopram and fluoxetine inhibited dSERT and also increased feeding and locomotion behaviors. At 100 mM, ketamine inhibited dSERT and increased serotonin concentrations, but decreased locomotion and feeding because of its anesthetic properties. Since microdosing ketamine causes behavioral effects, we further investigated behavioral changes with a SERT16 mutant and low doses of other NMDA receptor antagonists and 5‐HT1A and 2 agonists. Feeding and locomotion changes were similar to ketamine in the mutant, and we found NMDA receptor antagonism increased feeding, while serotonin receptor agonism increased locomotion, which could explain these effects with ketamine. Ultimately, this work shows that Drosophila is a good model to discern antidepressant mechanisms, and that ketamine does not work on dSERT like SSRIs, but effects behavior with other mechanisms that should be investigated further.image
Maternal supplementation of curcumin‐olive oil nanocomposite improves uteroplacental blood flow, placental growth and antioxidant capacity in goats
Authors: El‐Sherbiny Hossam R. , Samir Haney , Youssef Fady Sayed , Mohamed Gehad G. , Ismail Sameh H. , El‐Shahat Khaled H. , Aboelmaaty Amal M. , Mahrous Karima F. , Al Syaad Khalid M. , Ahmed Ahmed Ezzat , Al‐Saeed Fatimah A. , Abduallah Alduwish Manal , Abdelnaby Elshymaa A. 
Published: February 2024

AbstractThis experiment was designed to investigate the impact of curcumin‐olive oil nanocomposite (CONC) supplementation on uteroplacental hemodynamics and ultrasonographic measurements as well as maternal oxidative status in midgestating goats. Twelve synchronized pregnant goats (85.58 ± 1.08 days of gestation; mean ± SD) were uniformly assigned to two groups (n = 6/group); the first group received daily oral supplementation of CONC (3 mg/kg body weight; nanocurcumin [NC] group) for 32 days, and the second group was offered physiological saline (control) following the NC group timeline. The goats of both groups were examined at 3‐day intervals for middle uterine (MUA) and umbilical (UMA) arteries hemodynamics (pulsatility index [PI], resistive index [RI], systole/diastole [S/D] and blood flow rate [BFR]) and diameters, uteroplacental thickness (UPT), placentomes' diameter (PD) and echogenicity, steroid hormones (progesterone and estradiol 17β), oxidative biomarkers (total antioxidant capacity [TAC], catalase [CAT], malondialdehyde [MDA]), nitric oxide (NO) and blood cells DNA integrity. The UPT (p = 0.012) and PD (p = 0.021) values were higher in the NC group than in their counterparts' control group (D11–32). There were increases in diameter (p = 0.021 and p = 0.012) and decreases (p = 0.021, p = 0.016 and p = 0.041 [MUA]; p = 0.015, p = 0.023 and p = 0.011 [UMA] respectively) in Doppler indices (PI, RI and S/D) of the MUA and UMA in the NC group compared to the control group (D14–32). On D20–32 (MUA) and D14–32 (UMA), the NC goats had higher BFR than the control group (p = 0.021, 0.018 respectively). The means of blood cells with fragmented DNA were lower (p = 0.022) in the NC group than in the control group on Days 8 and 21 postsupplementation. There were increases in CAT and NO (D20–32; p = 0.022 and p = 0.004 respectively), and TAC (D17–32; p = 0.007) levels in the NC goats compared to the control ones. The NC group had lower (p = 0.029) concentrations of MDA than the control group on Day 20 postsupplementation onward. In conclusion, oral supplementation of CONC improved uteroplacental blood flow and the antioxidant capacity of midgestating goats.
Improving the swallowability of representative foods for the elderly and people with dysphagia
Authors: Qiu Xingbang , Zhong Min , Xu Wenhu 
Published: February 2024

AbstractIt is necessary to find food additives that can reduce the food friction coefficient, in order to make it easier for the elderly and people with dysphagia to swallow. The sesame paste and rice paste are two typical foods eaten by the elderly. In this research, the tribological and rheological properties of 2 paste with 12 hydrocolloids were investigated. The results showed that three hydrocolloids (xanthan gum, gellan gum, and pectin) could reduce the friction coefficients of sesame paste and rice paste within a certain range of oral friction velocity. The friction coefficients of two kinds of original paste are in the order of 10−1. The addition of three hydrocolloids can reduce them to about 10−2, effectively improving lubrication. After adding hydrocolloids, the particles cross‐link to form a network structure, which improves the viscosity and lubrication of food to a certain extent. This has potential application value for designing food that is more conducive to swallowing.
Hypothalamic‐pituitary‐adrenal axis recovery after intermediate‐acting glucocorticoid treatment in client‐owned dogs
Authors: Del Baldo Francesca , Corsini Andrea , Tardo Antonio Maria , Tirolo Alessandro , Sapignoli Ada , Tumbarello Michele , Vasylyeva Kateryna , Fracassi Federico 
Published: February 2024

AbstractBackgroundIn dogs, duration of hypothalamic‐pituitary‐adrenal (HPA) axis suppression after systemic glucocorticoid treatment is reported to vary from a few days to up to 7 weeks after glucocorticoid discontinuation. These data are derived mainly from experimental studies in healthy dogs and not from animals with spontaneous disease.Hypothesis and ObjectiveTo determine the timeline for recovery of the HPA axis in a group of ill dogs treated with intermediate‐acting glucocorticoids (IAGCs).AnimalsTwenty client‐owned dogs that received IAGC for at least 1 week.MethodsSingle‐center prospective observational study. An ACTH stimulation test, endogenous ACTH concentration, serum biochemistry profile, and urinalysis were performed at T0 (2‐6 days after IAGC discontinuation) and then every 2 weeks (eg, T1, T2, T3) until HPA axis recovery was documented (post‐ACTH cortisol concentration > 6 μg/dL).ResultsThe median time of HPA axis recovery was 3 days (range, 2‐133 days). Eleven of 20 dogs showed recovery of the HPA axis at T0, 6/20 at T1, and 1 dog each at T2, T5, and T9. Dose and duration of treatment were not correlated with timing of HPA axis recovery. Activities of ALT and ALP were significantly correlated with the post‐ACTH cortisol concentration (rs = −0.34, P = .03; rs = −0.31, P = .05). Endogenous ACTH concentration was significantly correlated with pre (r = 0.72; P < .0001) and post‐ACTH cortisol concentrations (r = 0.35; P = .02). The timing of HPA axis recovery of the dogs undergoing an alternate‐day tapering dose was not different compared to dogs that did not (3.5 vs 3 days, P = .89).Conclusion and Clinical ImportanceMost dogs experienced HPA axis recovery within a few days after IAGC discontinuation. However, 2/20 dogs required >8 weeks.