bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of various honey, fruits, and vegetables to increase hemoglobin levels in pregnant women with anemia in Indonesia
Authors: Widowati R 
Published: November 2023

Abstract
The aims of the study was to analyze the student’s theses research data of Midwifery Study Program at Faculty of Health Sciences Universitas Nasional regarding the use of honey, fruit and vegetables to increase hemoglobin levels in pregnant women with anemia in Indonesia. The research was conducted by analyzing student theses for four semesters between 2020 and 2023. The variables analyzed included location of the research, types of honey, fruits and vegetables, form of serving, method of consumption, and the effect on hemoglobin levels. A total of 522 student’s theses from 2020 until 2023 were recorded. The results showed 40 theses research (7.66%) were according to the research objectives. The research locations were cover 11 provinces in Indonesia. There are two types of honey, namely acacia honey and mutiflora honey. There are 10 fruits consumed with or without a combination (guava, star fruit, dates, tomatoes, dragon fruit, watermelon, ambon banana, papaya, lime, avocado). There are also 10 vegetables consumed with or without a combination (beetroot, carrot, red spinach, green spinach, katuk, moringa, matel, green bean, broccoli, and caulerpa). The mean increase in the hemoglobin level ranged from 0.85 to 3.71 g/dl. Prevention of anemia in pregnancy should not only be done by providing blood-boosting tablets or iron supplement but also by providing varied and nutritionally balanced food to increase the hemoglobin level and prevent anemia in pregnancy.
Profiling of HSPB9 gene sequences in Donggala (Bos indicus) and Polled Bali (Bos javanicus) cattle breeds of Indonesia
Authors: Hasbi H , Putra W P B , Maulana T , Agung P P , Said S , Sonjaya H , Baco S , Gustina S , Duma Y , Tanari M , Baharun A 
Published: November 2023

Abstract

HSPB9 gene plays important role to protect cells from heat stress in mammals. This research aim to describe the DNA sequence of HSPB9 gene in Donggala (Bos indicus) and Bali (Bos javanicus) bulls using forward sequencing method. A total of 10 blood samples from 6 Donggala and 4 Polled Bali were used in this study for DNA analysis. The bulls were kept at the breeding station at Central Sulawesi and South Sulawesi for Donggala and Bali, respectively. Results showed that a novel nonsence mutation of c.216C>T (p.L68L) was detected in the exon 1 of Bali HSPB9 gene. Commonly, the HSPB9 gene was monomorphic in Donggala and Bali cattle. In conclusion, the mutation of c.216C>T might be used as the genetic marker to discriminate Donggala and Bali cattle.
Somatic embryo formation, plantlet vigorous and greenhouse preliminary test of garlic bulblet obtained through somatic embryogenesis
Authors: Supriyati Y , Tambunan I R , Mastur  , Tamami J , Azizi A A A , Darsa Y , Yufdi P 
Published: November 2023

Abstract
Garlic production in Indonesia is still low due to limited availability of seedling. Approximately 10% of garlic yield are used for the next cultivation. Therefore about 90 % of garlic consumption are still imported from China. In addition to conventional seed production, alternative routes are needed, such as through somatic embryogenesis. Research consists of three activities, namely 1) induction and multiplication of callus, 2) embryo somatic and plantlet formation and 3) preliminary test of bulblet in vitro in the greenhouse. Parameters observed in the laboratory activities were time of callus emergence, callus weight and morphology, time of somatic embryo emergence, embryo diameter and weight per bottle, plantlet number and vigorous and bulblet number per bottle, while in the greenhouse, parameter observed were survival of bulblet number, plant height, leaf number per plant, and clove number and weight. The result showed that application of 2,4-D 3 mg/l with casein hydrolysate acid was suitable for callus induction of garlic var. Lumbu Kuning, while BAP 2 mg/l was the most suitable formula for callus multiplication, the addition of paclobutrazol 1 ppm and sucrose 20% in MS medium was the most suitable treatment for plantlet vigorous, while the bulblet formation could be developed by using jasmonic acid with sucrose of 12%. The best medium for growjng media of in vitro bulblet in the greenhouse was the combination of sandy soil, cocopeat and manure.
Segregation of molecular markers associated with Bph3 gene in BC5F2 population derived from Ciherang rice variety as the recipient parent
Authors: Yunus M , Dadang A , Slamet  , Warsun A , Satyawan D , Chaerani  
Published: November 2023

Abstract
Brown planthopper (BPH), an important insect pest of rice, can cause severe damage and significant yield loss. An efficient and environmentally friendly method to control this pest is by planting resistant varieties. The brown planthopper resistance gene Bph3 from Rathu Heenati variety is closely linked to the Waxy gene that regulates amylose synthesis, the primary determinant of rice eating and cooking quality. The purposes of this study were to analyze the segregation ratio of SSR markers associated with Bph3 gene and to identify individual plants carrying homozygous allele for Bph3 gene in a BC5F2 population derived from a backcross that used Ciherang as the recurrent recipient parent. Analyses using RM589, RM586, and RM588 markers linked to Bph3 gene and RM190 marker linked to the Waxy gene indicated that the ratio of genotype segregation for each marker related to Bph3 gene did not deviate from the expected ratio with the χ
2 value was 0.956, 0.587, and 1.467 for RM589, RM586, and RM588, respectively (χ2
table = 5.991, α=0,05). All individual plants had a homozygous allele from Ciherang for Waxy gene based on the RM190 marker. As many as 25 individual plants which have homozygous Bph3 allele from Rathu Heenati and homozygous Waxy allele from Ciherang can be further developed as promising BPH-resistant rice varieties with eating and cooking quality similar to Ciherang.
Metabolite profiling of the medicinal herb Phyllanthus niruri L. under drought stress
Authors: Nawfetrias W , Nurhangga E , Reninta R , Chotimah S , Bidara I S , Maretta D , Devy L , Esyanti R R , Faizal A 
Published: November 2023

Abstract
Plant-specialized metabolites are unique sources for pharmaceuticals and industrially valuable biochemicals. Accumulation of these metabolites is common in plants stimulated by biotic or abiotic stressors and/or elicitors. One of the abiotic factors that influence the plant’s bioactive molecule is drought stress, which can be employed to stimulate the production of bioactive compounds in plants. Phyllanthus niruri is commonly used as a medicinal plant in many parts of the world for the treatment of various diseases because it contains bioactive compounds that have the potential to be antioxidants, antimicrobials, and anti-hepatoprotective. Our previous studies have demonstrated that drought stress up to 70% field capacity (FC) could increase crop production as well as phenol and flavonoid contents. This study aimed to analyze the metabolite profile of P. niruri, which was subjected to drought stress for various FC (Control, 85%, 80%, 75%, 70%, 55%). Using chromatography-mass spectrophotometry (GC-MS) analysis, forty-seven compounds were identified. Three compounds were detected in control and all treatments, whereas four compounds were only detected in drought-stressed plants. β-sitosterol, 9-tricosane, heneicosane, and hexacosane are the main compounds only detected at 80% FC. The clustering analysis revealed that drought stress induced distinct compounds compared to control. Moreover, 85% -75% FC presumably induces similar compounds as they were in the same cluster, while plants responded differently upon 70% FC and 55% FC. In conclusion, P. niruri synthesizes different bioactive compounds under different drought-stress conditions.
SSR diversity on rice landraces collected from Yogyakarta Province
Authors: Kurniawan H , Hidayatun N , Kristamtini  , Widyayanti S , Risliawati A 
Published: November 2023

Abstract
In Indonesia, rice is the most common food, and many areas have a lot of rice landraces. Therefore, characterization is very important to be done to identify potential gene sources to be used for the breeding program. The current study aims to learn more about the genetic diversity of rice landrace accessions collected from Yogyakarta province using simple sequence repeat (SSR) markers. A total of 56 accessions of rice landraces originated from Yogyakarta province, and 7 accessions of standard varieties, were used in the study. The DNA sample was extracted from young leaves and isolated for further molecular analysis by using eleven SSR markers. Data analysis was conducted using Power Marker v3.25 for genetic analysis, and DARwin v6.0.021 for cluster analysis and dendrogram visualization. The results of the genetic analysis showed that the average values of major allele frequency, number of alleles, gene diversity, heterozygosity, and polymorphic information content (PIC) were 0.27, 10.1, 0.83, 0.15, and 0.80, respectively. All eleven SSR markers were highly informative (PIC>0.50), as their PIC values ranged from 0.69 to 0.85. These markers have great potential to be used for marker-assisted selection of the germplasm collections. The result of cluster analysis showed that all the rice landrace accessions were separated into three different groups, each consisting of 29, 28, and 6 accessions.
Tobacco resistance gene expression levels in response to the infection of Phytophthora nicotianae
Authors: Sukweenadhi J , Christian C V , Setiabudi J , Savitri W D 
Published: November 2023

Abstract
One of the main obstacles often encountered in efforts to increase productivity and quality of tobacco (Nicotiana tabacum) yields is the attack of black shank disease by the oomycetes Phytophthora nicotianae. So far, what has been done mainly by tobacco cultivators is the observation of resistance through calculating the rate of death or disease of plants due to pathogen attack, so research is needed to determine the resistance profile of local varieties of tobacco to black shank disease in molecular stage to speed up the screening process of tobacco varieties. This study aim to determine the gene expression profile related to resistance in tobacco varieties Beinhart 1000, Hick Broadleaf, and two local varieties, namely Dark 302 and Dark 314, in response to black shank disease. The research consisted of preparing tobacco and P. nicotianae inoculum, artificial inoculation, followed by semi-quantitative analysis of gene expression related to tobacco resistance, PR1, PR4, PLP2, and PUB24. Tobacco resistant variety, Beinhart 1000, expressed a relatively 285% higher PR1 significantly than the control. The length of time of infection showed that PR1, PR4, PLP2, and PUB24 genes were expressed temporally, and the dynamics of expression of each test gene affected the development of pathological conditions.
Callus stage and morphology affect the DNA yield on the DNA extraction from the sugarcane in vitro callus
Authors: Aksa A A , Setiawati Y , Minarsih H , Riyadi I , Prasetyo M E R B , Sinta M M , Sumaryono  , Saptari R T 
Published: November 2023

AbstractThe main concern with the micropropagation of sugarcane (Saccharum officinarum) with a complex polyploid genome is the frequent occurrence of somaclonal variations. Therefore, genetic stability analysis is often required to ensure the uniformity of the micropropagated sugarcane, as early as in the callus stage. DNA extraction is the initial and important step of the genetic stability analysis. In this experiment, we compared the yield of DNA extraction from the sugarcane embryogenic and non-embryogenic calli. The sugarcane non-embryogenic callus was initiated from immature rolled leaf explants of the 6 months-old plant cane in the callus induction media, that were incubated in the dark room. The embryogenic calli were obtained by routine subcultures of the non-embryogenic calli every six weeks. The results showed that the extraction yield from the non-embryogenic calli was far lower than that from the embryogenic calli, most likely due to the high-water content of the non-embryogenic calli. Furthermore, histological analysis using Safranin and Fast Green staining revealed the low lignin content and nuclei in the non-embryogenic calli cell mass, causing the transparent and watery appearance of the calli and might influence the low DNA extraction yield.
Profiles of Semi-Polar Metabolites from Leaves of In Vitro- Derived Plants of Indonesian Pepper Varieties (Capsicum annuum) after Gamma Irradiation Treatments
Authors: Wahyuni  , Sri Hartati N , Sulistyowati Yuli , Perdani Ambar Y , Roziqoh Wasiatur , Farias-Pereira Renalison , Su Xiaoyang , Sudarmonowati Enny , Raskin Ilya 
Published: November 2023

Abstract
Gamma irradiation has been widely utilized for mutation breeding as it induces random mutations in plant cells. Due to limitations on other breeding approaches, including cross-breeding and transgenic plants, gamma irradiation-induced mutation breeding has regained its popularity among breeders and scientists. Untargeted metabolomics analysis can be used to profile any perturbation between untreated samples and treated sample groups without having prior knowledge of particular metabolites, including semi-polar metabolites, which cover phenolic acids, flavonoids, glycosylated steroids, alkaloids, and other glycosylated species. This study aimed to profile semi-polar metabolite modification in leaves of plants derived from in vitro cultures after gamma irradiation treatments in Indonesian chili pepper varieties. Seeds of Laris and Kopay were exposed to gamma-ray doses (0, 100, 200, 300, 400, and 500 Gy), after which they were germinated and cultivated in vitro. Plantlets were acclimated in the greenhouse and leaves were collected for metabolite analysis at 80 days after the germination of irradiated seeds. Semi-polar metabolites from leaves were extracted using methanol and the extracts were subjected to LC-MS analysis. Results showed that the composition and levels of semi-polar metabolites of Laris 100, 200, 300, 400, and 500 Gy were similar to Laris 0 Gy (control; unirradiated seeds), although several abundancies of that of 200 Gy gamma irradiation dose were different than control. Except at dose 400 Gy, Kopay at 0 Gy, 200 Gy, and 300 Gy also showed similar metabolite profiles, indicating that gamma-ray doses did not induce mutation at genes regulating metabolite biosynthetic pathways. In contrast, Kopay 400 Gy showed low levels of terpenoids and flavonoids, indicating that a 400 Gy dose of gamma ray may affect the upstream part of the shikimate biosynthetic pathway, resulting in low levels of precursors at the upstream biosynthetic pathway of terpenoids and flavonoids. Hence, the accumulation of terpenoids and flavonoids was very low. These findings provide insights into the effect of gamma irradiation for mutation breeding that may be important for future pepper breeding programs.
Transformation of CRISPR/Cas9 expression construct to enhance saturated fatty acid synthesis in oil palm for biofuel production
Authors: Minarsih H , Permatasari G W , Setiawati Y , Mardhika L D , Saptari R T , Riyadi I , Aksa A A , Sinta M M 
Published: November 2023

Abstract
New technology innovations and the utilization of elite varieties are necessary for oil palm intensification to improve productivity. In addition to productivity improvement, oil palm intensification is also directed to improve the quality of crude palm oil (CPO) to be used in biofuel production. The modification of the fatty acid biosynthesis pathway through genetic engineering is one of the most promising approaches. The research aims to apply a genetic transformation to oil palm calli and to improve success regeneration of the transformed explants. A CRISPR/Cas9 expression construct to edit the PTE (palmitoyl-acyl carrier protein thioesterase) gene, which protein is involved in the formation of palmitic acid, was generated in the previous research. In this study, the genetic transformation of the construct into 4month-old oil palm calli was conducted via Agrobacterium tumefaciens. The transformed calli were subsequently subcultured into selection media containing 100 ppm of cefotaxime and 50 ppm of kanamycin antibiotics. After several weeks, the transformed calli were transferred into DF10 media without antibiotics. Non-transformed calli were able to grow on the selection media with antibiotics but still in the globular phase. In contrast, the transformed calli on the selection media were able to grow to the scutellar phase with a compact structure in the embryogenic callus development stages. The next step is to optimize the transformation method as well as the regeneration media composition.
Application of combination of auxins in callus induction and proliferation of shallot cv Trisula and Sumenep through in vitro technique
Authors: Rahayu S , Purnamaningsih R , Husni A , Chaerani  , Rosliani R 
Published: November 2023

Abstract
Shallot (Allium cepa L. var. aggregatum) is one of a strategic commodity in Indonesia. To increase of production of shallot seed, there needs a good tuber with high productivity tolerant to abiotic stress. One of the obstacles to increase of shallot production with tuber in good quality and tolerant to abiotic stress is not available. Tissue culture technique is one of the methods proved can propagate plant in large amounts and in a shorter time compared to the conventional method. The objectives of this research are to get the best media formulation for callus induction and proliferation. The explant used in this research are shoot tip of shallot from cultivar Trisula and Sumenep. The Media used were MS. The medium was enriched with (0,5 – 3 mg/l) 2,4-D + 2 mg/l picloram (0,5 – 3 mg/l) or NAA + with or without 3 gr/l Casein hydrolysate; and MS + 3 mg/l Picloram + 2 or 3 mg/l NAA + 3 g/l Casein Hydrolysate. The best media formulation for callus induction of Trisula variety was MS + 3 mg/l 2,4D + 2 mg/l picloram + 3 g/l Casein Hydrolysate, whereas the best media formulation for callus induction of Sumenep variety was MS + 0.5 mg/l 2,4-D + 2 mg/l picloram. The best media formulation for callusproliferation was MS + 0.5 mg/l 2,4-D + 2 mg/l picloram both Trisula and Sumenep varieties. These results could be used for plant propagation and breeding program of shallot in the future.
Exploration and macroscopic characterization of microbiomes (fungi and bacteria) on anthracnose-Infected chili plants in lowlands fields of Deli Serdang North Sumatra
Authors: Tarigan R , Hermanto C , Lisnawita  , Safni I , Udiarto B K , Hasim A , Setiawati W , Marpaung A E , Sebayang A , Riska  , Karo B , Barus S , Hutabarat R C , Resiani D. , Jumjunidang  
Published: November 2023

AbstractAnthracnose is one of the main obstacles in chili cultivation. This disease reduces chili production by 40% to 100% during the rainy season. Most techniques used to control anthracnose is using synthetic fungicides that potentially cause resistance and environmental pollution. Disease management by the microbiome of plants with antagonistic properties is an alternative biological control. The aim of the study was to explore the diversity of the microbiome (fungi and bacteria) in various tissues (rhizosphere, endosphereandphylosphere) of large red chili plants infected with anthracnose macroscopically. The object of this research was the leaves, stems, and roots of 5 months old of chili plants affected by anthracnose from two different locations of planting areas. The exploration of the microbiome of anthracnose- infected chili plants in a lowland area in Sumatera Utara in 3 DAI discovered 13 fungi and 12 bacteria, whilst 10 fungi and 11 bacteria in the second location by tissue cropping technique. Besides that, by grinding technique, resulted in 14 fungi isolates dan 13 bacteria in the first location and 16 fungi isolates and 14 bacteria in the second location. At 7 DAI, there found 130 bacteria in the first location and 146 bacteria in location 2 by grinding technique. By tissue cropping technique, there were 124 bacteria in the first location and 102 bacteria in the second location. Morphologically, fungi were identified asFusariumsp, Aspergillussp,Penicillumsp,Trichodermasp,Humicolasp,Colleotetrichumsp,Idriellasp,Alternariasp,Rhizopussp andCurvulariasp while the bacteria obtained 9 different isolates based on the morphology of colony.
Lyoprotectants for Lactobacillus brevis B144 and their capability to maintain the expression of surface layer protein
Authors: Pratiwi R D , Zanjabila S 
Published: November 2023

Abstract

Lactobacillus brevis as a probiotic has been reported to facilitate micronutrient absorption such as minerals (zinc, calcium, magnesium) into the intestine through the surface layer protein (Slp). Negatively charged-Slp from the bacteria that adheres on the intestinal mucosa binds to the positively charged-minerals, e.g., calcium, zinc, iron, magnesium and potassium via an ionic interaction. In this study, several sugar-based lyoprotectants (glucose, galactose, and lactose) and skim milk were implemented in the L. brevis B144 InaCC freeze drying. The dried L. brevis B144 InaCC was kept in the refrigerator up to three months and monthly tested for the cell viability. The dried L. brevis B144 InaCC was re-culture and then its Slp was isolated using 8 M guanidine hydrochloride. In general, combinations of skim milk and the sugar-based lyoprotectant provided higher protection than the skim milk alone. Meanwhile, without any lyoprotectant, the L. brevis B144 InaCC lose its ability to grow. In regards to maintain the capability of the Slp expression, the L. brevis B144 InaCC which was protected with the combination of 20% skim milk and 10% galactose showed the highest protein expression level among the other lyoprotectants.
The nano mixture of ethanol extract ketumbar (Coriandrum sativum L.) seed and putri malu (Mimosa pudica L.) leaves potentially reduced blood glucose levels in hyperglycemia wistar rat
Authors: Wahjuni S , Puspawati N M , Manuaba I B P 
Published: November 2023

Abstract
The formation of nano extracts can increase the bioactivity of a compound in the body. The purpose of this study was to determine the ability of a nano mixture of Coriandrum sativum L seed and ethanol extract of Mimosa pudica L leaves to reduce glucose levels in hyperglycemic rats, compared to an ethanolic extract mixture of Coriandrum sativum L seeds and Mimosa pudica L leaves. The size and zeta potential characterization of the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves was carried out using a Particle Size Analyzer (PSA). The results of characterization with PSA showed that the nanoparticle size of the ethanolic mixture of Coriandrum sativum L seeds and Mimosa pudica L leaves was 184.4 nm and the zeta potential value was -1.62 mV. The particle size obtained is in accordance with the herbal nano size, which is 50-300 nm. Identification of compounds in the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves by LC-MS/MS, found 7 compounds suspected of antihyperglycemia, namely phenylalanine, indole, 4-methoxy benzaldehyde, 2,5-dimethoxy benzaldehyde, apigenin, 2-flouren-9-ylidenemethyl-pyridine, and 5-Pentyl-1,3-benzenediol. The activity test results showed that the nano ethanol extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves was more effective in lowering blood glucose levels (79.29%) compared to the ethanolic extract mixture of Coriandrum sativum L seed and Mimosa pudica L leaves (50.88%).