bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pediatric Intersession: An Upfront Flipped-Classroom Curriculum to Promote Pediatric Clerkship Readiness
Authors: Congdon Morgan , Goldstein Laura , Maletsky Kristin D. , Craven Meghan , Rose Stacey , Devon Erin Pete 
Published: February 2024

OBJECTIVES Best practices for content selection, mode of delivery, and timing of pediatric clerkship readiness curricula for medical students have, by and large, not been established. Capitalizing on changes in structure of the clinical clerkships during the COVID-19 pandemic, we created an upfront clerkship readiness curriculum, termed Pediatric Intersession (PI), to replace the existing weekly lecture-based clerkship didactics. METHODS Our goal was to develop an interactive curriculum with innovative instructional design methodology intended to promote broad foundational pediatric knowledge and clerkship preparedness using case-based learning. We first conducted a needs assessment and crafted curriculum content using guiding principles from the 2019 Council on Medical Student Education in Pediatrics (COMSEP) curriculum. We then organized material into four daily modules prior to the start of the clerkship and employed flipped classroom (FC) methodology. RESULTS Sixty-six percent of students completed course evaluations, and >90% of the 100 respondents reported that the PI enhanced their clinical learning. Pre-/post-testing demonstrated some knowledge gain following the small-group sessions and there was no change on the National Board of Medical Examiners (NBME) Subject Exam mean scores compared to prior cohorts of students. CONCLUSIONS The global pandemic provided an opportunity to re-envision our pediatric clerkship didactics content, while also incorporating instructional design methodology preferred by students. Our curriculum promotes a small group-based, interactive approach to clerkship readiness that fosters learning in the clinical environment that can be adapted for various settings. Our evaluation suggests that the transition to a FC readiness curriculum can be done successfully while effectively preparing students for their pediatric clerkship.
Exploring Factors of Sexual Function and Sexual Satisfaction Among Nonworking Women: A Qualitative Study
Authors: Pious Anu Anns , Amaresha Anekal C. 
Published: February 2024

Background: Sexual function and satisfaction are 2 important components of sexual health. Both sexual function and satisfaction of women are influenced by various internal and external factors over their life cycle. This study aims to explore the factors of sexual function and satisfaction among nonworking married women in Bengaluru using a qualitative exploratory study. Materials and Methods: This is a qualitative exploratory research study that adopted an inductive thematic data analysis. In-depth qualitative interviews were conducted with 11 nonworking working married women of Bengaluru. The interviews were audio recorded, and the transcribed data were analyzed with ATLAS.ti software. The results were presented thematically. Results: It was found that somatic and personal factors such as health and appearance, pregnancy and postpregnancy issues, compatibility between couples, and spousal qualities; psychological factors such as stressors and stabilizers that related to emotional and mental health; and situational and extrinsic factors such as pressure to conceive, child-rearing, types of family, traditional beliefs and practices, societal stigma and taboo, daily schedules of couples, and ambiance and privacy were influencing the sexual function and satisfaction of women. Conclusion: The study could find positive and negative factors of sexual function and satisfaction. These factors need further exploration with larger studies from other cultures and groups.
Kalyuzhnyite-(Ce), NaKCaSrCeTi(Si8O21)OF(H2O)3, a new mineral from the Darai-Pioz alkaline massif, Tien-Shan mountains, Tajikistan: mineral description, crystal structure and a new double (Si8O21) sheet
Authors: Agakhanov Atali A. , Sokolova Elena , Karpenko Vladimir Yu. , Hawthorne Frank C. , Pautov Leonid A. , Kasatkin Anatoly V. , Pekov Igor V. , Agakhanova Vitaliya A. 
Published: February 2024

AbstractKalyuzhnyite-(Ce), ideally NaKCaSrCeTi(Si8O21)OF(H2O)3, is a new mineral from the Darai-Pioz alkaline massif, Tien-Shan mountains, Tajikistan. It occurs as equant grains up to 0.05 × 0.07 mm in a quartz–pectolite aggregate in a silexite-like peralkaline pegmatite. Associated minerals are quartz, fluorite, pectolite, baratovite, aegirine, leucosphenite, neptunite, reedmergnerite, orlovite, sokolovaite, mendeleevite-(Ce), odigitriaite, pekovite, zeravshanite, kirchhoffite and garmite. The mineral is colourless with a vitreous lustre and a white streak, and Dcalc. is 3.120 g/cm3. Kalyuzhnyite-(Ce) is monoclinic, P2/c, a = 18.647(4), b = 11.214(2), c = 14.642(3) Å, β = 129.55(3)° and V = 2360.9(11) Å3. The chemical composition of kalyuzhnyite-(Ce) is Nb2O5 0.53, TiO2 0.16, SiO2 43.85, Er2O3 0.13, Ho2O3 0.10, Gd2O3 0.09, Sm2O3 0.47, Nd2O3 6.22, Pr2O3 1.21, Ce2O3 6.34, La2O3 0.82, PbO 4.90, BaO 0.85, SrO 11.39, CaO 1.86, Cs2O 3.80, K2O 1.59, Na2O 2.99, H2O 5.24, F 1.55, O = F –0.65, total 100.31 wt.%. The empirical formula calculated on 26.11 (O + F) apfu is Na1.07K0.37Cs0.30Sr1.21Ca0.37Pb0.24Ba0.06(Ce0.43Nd0.41Pr0.08La0.06Sm0.03Gd0.01Er0.01Ho0.01)Σ1.04(Ti0.97Nb0.04)Σ1.01Si8.06O25.21F0.90H6.42, Z = 4. The simplified formula is (Na,□)(K,Сs)(Ca,Pb,Sr,Na)SrLn3+Ti(Si8O21)OF(H2O)3, where Ce is the dominant lanthanoid. The crystal structure was solved by direct methods and refined to an R1 index of 2.74%. In kalyuzhnyite-(Ce), the main structural units are a heteropolyhedral Na–Sr–Ce–Ti sheet, ideally [NaSrCeTiOF]7+, and a double (Si8O21)10– sheet parallel to (010). In the Si–O sheet, the Si tetrahedra form ten-membered rings. This is the first occurrence of such a double Si–O sheet in a mineral. The two sheets connect via common vertices of Na-, Sr-, Ce- and Ti-polyhedra and SiO4 tetrahedra to form a framework. The interstitial cations and H2O groups, ideally [(CaK)(H2O)3]3+, occur within the Si–O sheet. The mineral is named in honour of Vasily Avksentievich Kalyuzhny (1899–1993) in recognition of his contributions to the geology of ore deposits of Komi Republic (USSR) and the mineralogy of granitic pegmatites (Tajikistan).
Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)
Authors: Hagaggi Noura Sh. A. , Abdul-Raouf Usama M. , Radwan Tarek A. A. 
Published: February 2024

Abstract
Background
Acacia species are economically significant as medicinal plants that have been utilized since ancient times. Acacia modesta has been reported to possess potent antibacterial and antioxidant properties, but its growth rate is slow. In this study, we hypothesized that inducing callus in vitro from A. modesta could enhance the production of antibacterial and antioxidant secondary metabolites, thereby circumventing the issues of slow growth and excessive harvesting of the plant.

Results
The callus was induced from axillary buds on MS medium supplemented with 1 mg/L of 2,4-D and 1 mg/L of BAP. The secondary metabolites, volatile compounds, antibacterial activity, and antioxidant activity of the callus and parent plant leaf extracts were evaluated. The results revealed that the content of phenolics and flavonoids, the number of volatile compounds, and the antibacterial and antioxidant activities of the callus extract were significantly enhanced (P ≤ 0.05) compared to the leaf extract. The antibacterial and antioxidant effects were strongly correlated with the total phenolic and flavonoid content in the extracts.

Conclusions
Our findings suggest that in vitro callus culture increases the production of phenolics, flavonoids, and volatile compounds. This subsequently enhances the antibacterial and antioxidant properties of A. modesta.
Genomic survey and expression analysis of LcARFs reveal multiple functions to somatic embryogenesis in Liriodendron
Authors: Xu Lin , Liu Ye , Zhang Jiaji , Wu Weihuang , Hao Zhaodong , He Shichan , Li Yiran , Shi Jisen , Chen Jinhui 
Published: February 2024

Abstract
Background
Auxin response factors (ARFs) are critical transcription factors that mediate the auxin signaling pathway and are essential for regulating plant growth. However, there is a lack of understanding regarding the ARF gene family in Liriodendron chinense, a vital species in landscaping and economics. Thus, further research is needed to explore the roles of ARFs in L. chinense and their potential applications in plant development.

Result
In this study, we have identified 20 LcARF genes that belong to three subfamilies in the genome of L. chinense. The analysis of their conserved domains, gene structure, and phylogeny suggests that LcARFs may be evolutionarily conserved and functionally similar to other plant ARFs. The expression of LcARFs varies in different tissues. Additionally, they are also involved in different developmental stages of somatic embryogenesis. Overexpression of LcARF1, LcARF2a, and LcARF5 led to increased activity within callus. Additionally, our promoter-GFP fusion study indicated that LcARF1 may play a role in embryogenesis. Overall, this study provides insights into the functions of LcARFs in plant development and embryogenesis, which could facilitate the improvement of somatic embryogenesis in L. chinense.

Conclusion
The research findings presented in this study shed light on the regulatory roles of LcARFs in somatic embryogenesis in L. chinense and may aid in accelerating the breeding process of this tree species. By identifying the specific LcARFs involved in different stages of somatic embryogenesis, this study provides a basis for developing targeted breeding strategies aimed at optimizing somatic embryogenesis in L. chinense, which holds great potential for improving the growth and productivity of this economically important species.
Prevalence and modifiable risk factors of cognitive frailty in patients with chronic heart failure in China: a cross-sectional study
Authors: Xu Jiayi , Xiang Luwei , Zhang Huichao , Sun Xing , Xu Dongmei , Wu Die , Chen Chen , Zhang Yixiong , Gu Zejuan 
Published: February 2024

Abstract
Background
Cognitive frailty (CF) is currently a significant issue, and most of the associated factors discovered in current studies are not modifiable. Therefore, it is crucial to identify modifiable risk factors that can be targeted for interventions in patients with chronic heart failure (CHF). This study aimed to investigate the prevalence and modifiable risk factors of CF in CHF patients in China.

Methods
In this cross-sectional study, we sequentially enrolled patients diagnosed with CHF. CF served as the dependent variable, assessed through the Montreal Cognitive Assessment (MoCA) Scale and the FRAIL Scale. The independent variable questionnaire encompassed various components, including general demographic information, the Social Support Rating Scale (SSRS), the Simplified Nutrition Appetite Questionnaire (SNAQ), the Hamilton Depression Scale (HAMD), the Hamilton Anxiety Scale (HAMA), and the Minnesota Living with Heart Failure Questionnaire (MLHFQ). Logistic regression analysis was employed to identify independent factors contributing to CF.

Results
A total of 271 patients with CHF were included in the study. The overall prevalence of CF was found to be 49.4%, with 28.8% of patients exhibiting potentially reversible cognitive frailty and 20.7% showing reversible cognitive frailty. Among middle-young CHF patients, 10.7% had reversible cognitive frailty and 6.4% had potentially reversible cognitive frailty, with a prevalence of CF at 17.1%. Logistic regression analysis revealed that body mass index (OR = 0.826, 95%CI = 0.726–0.938), blood pressure level (OR = 2.323, 95%CI = 1.105–4.882), nutrition status (OR = 0.820, 95%CI = 0.671–0.979), and social support (OR = 0.745, 95%CI = 0.659–0.842) were independent factors associated with CF (p < 0.05).

Conclusions
We observed a relatively high prevalence of CF among Chinese patients diagnosed with CHF. Many factors including BMI, blood pressure level, nutrition status, and social support emerging as modifiable risk factors associated with CF. We propose conducting clinical trials to assess the impact of modifying these risk factors. The outcomes of this study offer valuable insights for healthcare professionals, guiding them in implementing effective measures to improve the CF status in CHF patients during clinical practice.
Relative effects of climate factors and malaria control interventions on changes of parasitaemia risk in Burkina Faso from 2014 to 2017/2018
Authors: Traoré Nafissatou , Singhal Taru , Millogo Ourohiré , Sié Ali , Utzinger Jürg , Vounatsou Penelope 
Published: February 2024

AbstractBackgroundIn Burkina Faso, the prevalence of malaria has decreased over the past two decades, following the scale-up of control interventions. The successful development of malaria parasites depends on several climatic factors. Intervention gains may be reversed by changes in climatic factors. In this study, we investigated the role of malaria control interventions and climatic factors in influencing changes in the risk of malaria parasitaemia.MethodsBayesian logistic geostatistical models were fitted on Malaria Indicator Survey data from Burkina Faso obtained in 2014 and 2017/2018 to estimate the effects of malaria control interventions and climatic factors on the temporal changes of malaria parasite prevalence. Additionally, intervention effects were assessed at regional level, using a spatially varying coefficients model.ResultsTemperature showed a statistically important negative association with the geographic distribution of parasitaemia prevalence in both surveys; however, the effects of insecticide-treated nets (ITNs) use was negative and statistically important only in 2017/2018. Overall, the estimated number of infected children under the age of 5 years decreased from 704,202 in 2014 to 290,189 in 2017/2018. The use of ITNs was related to the decline at national and regional level, but coverage with artemisinin-based combination therapy only at regional level.ConclusionInterventions contributed more than climatic factors to the observed change of parasitaemia risk in Burkina Faso during the period of 2014 to 2017/2018. Intervention effects varied in space. Longer time series analyses are warranted to determine the differential effect of a changing climate on malaria parasitaemia risk.
Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes
Authors: Dung Tran Thi Ngoc , Phat Voong Vinh , Vinh Chau , Lan Nguyen Phu Huong , Phuong Nguyen Luong Nha , Ngan Le Thi Quynh , Thwaites Guy , Thwaites Louise , Rabaa Maia , Nguyen Anh T. K. , Duy Pham Thanh 
Published: February 2024

Abstract
Background
Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus are major bacterial causes of lower respiratory tract infections (LRTIs) globally, leading to substantial morbidity and mortality. The rapid increase of antimicrobial resistance (AMR) in these pathogens poses significant challenges for their effective antibiotic therapy. In low-resourced settings, patients with LRTIs are prescribed antibiotics empirically while awaiting several days for culture results. Rapid pathogen and AMR gene detection could prompt optimal antibiotic use and improve outcomes.

Methods
Here, we developed multiplex quantitative real-time PCR using EvaGreen dye and melting curve analysis to rapidly identify six major pathogens and fourteen AMR genes directly from respiratory samples. The reproducibility, linearity, limit of detection (LOD) of real-time PCR assays for pathogen detection were evaluated using DNA control mixes and spiked tracheal aspirate. The performance of RT-PCR assays was subsequently compared with the gold standard, conventional culture on 50 tracheal aspirate and sputum specimens of ICU patients.

Results
The sensitivity of RT-PCR assays was 100% for K. pneumoniae, A. baumannii, P. aeruginosa, E. coli and 63.6% for S. aureus and the specificity ranged from 87.5% to 97.6%. The kappa correlation values of all pathogens between the two methods varied from 0.63 to 0.95. The limit of detection of target bacteria was 1600 CFU/ml. The quantitative results from the PCR assays demonstrated 100% concordance with quantitative culture of tracheal aspirates. Compared to culture, PCR assays exhibited higher sensitivity in detecting mixed infections and S. pneumoniae. There was a high level of concordance between the detection of AMR gene and AMR phenotype in single infections.

Conclusions
Our multiplex quantitative RT-PCR assays are fast and simple, but sensitive and specific in detecting six bacterial pathogens of LRTIs and their antimicrobial resistance genes and should be further evaluated for clinical utility.
Molecular examination for Coxiella burnetii and Brucella spp. infections in Iranian women experiencing spontaneous miscarriage
Authors: Baseri Neda , Omidi Amir Hossein , Latifian Mina , Mostafavi Ehsan , Khademvatan Shahram , Omidifar Navid , Tabaei Seyyed javad Seyyed , Jafari Rasool , Zeinali Shiva , Ghasemi Ahmad , Esmaeili Saber 
Published: February 2024

Abstract
Background
Spontaneous miscarriage, a leading health concern globally, often occurs due to various factors, including infections. Among these, Coxiella burnetii and Brucella spp. may have adverse effects on pregnancy outcomes. While previous research has established a link between infections and spontaneous miscarriage, our study aimed specifically to investigate the presence of these two pathogens in abortion samples from women who experienced spontaneous miscarriages in Iran. Our study can add to the existing knowledge by focusing on Iran, a region with a high prevalence of C. burnetii and Brucella spp. As a result, it could provide a better understanding and unique insights into the relationship of these pathogens with spontaneous miscarriages in endemic regions.

Methods
From March 2021 to March 2022, a total of 728 abortion samples (including placenta and cotyledon) were collected from 409 women who had experienced spontaneous miscarriages in the provinces of Tehran, Fars, and West Azerbaijan in Iran. The specimens included 467 Formalin-Fixed Paraffin-Embedded (FFPE) and 261 fresh frozen samples. After DNA extraction from abortion samples, the quantitative real-time PCR (qPCR) assay targeted a specific fragment of the IS1111 and IS711 elements for molecular identification of C. burnetii and Brucella spp., respectively. Furthermore, the qPCR assay employing specific primers for different species was used to determine the species of Brucella.

Results
Among the studied women, 1 out of 409 (0.24%) samples tested positive for Brucella spp., specifically Brucella melitensis. There were no positive specimens for C. burnetii.

Conclusions
Our study contributes to understanding the potential involvement of Brucella species in spontaneous infectious abortion within endemic regions. The identification of B. melitensis in this study highlights the need for further research in this area. However, while our results suggest a relatively low or zero identification of these pathogens in our sample population, this does not rule out the possibility of undetected infections. Therefore, it is critical to acknowledge the limitations of the molecular techniques used (qPCR), which may have potential limitations such as sensitivity and specificity. Moreover, because 64.15% of our samples were FFPE, the sensitivity of the qPCR test may be reduced. These raise concerns about the accuracy of the reported prevalence rates and the potential for false positives or negatives.
Risk of transmission of SARS-CoV-2 on international flights, a retrospective cohort study using national surveillance data in England
Authors: Howkins Joshua , Packer Simon , Walsh Eleanor , Kumar Deepti , Edeghere Obaghe , Hickman Matthew , Oliver Isabel 
Published: February 2024

Abstract
Background
It is not yet fully understood to what extent in-flight transmission contributed to the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). This study aimed to determine the occurrence and extent of SARS-CoV-2 transmission in-flight and assess factors associated with transmission risk to inform future control strategies.

Methods
Retrospective cohort study using data obtained from contact tracing of international flights arriving in England between 02/08/2021–15/10/2021. Transmission risk was estimated by calculating the secondary attack rate (SAR). Univariable and multivariable analyses of the SAR by specific risk factors was undertaken, including: number of in-flight index cases; number of symptomatic index cases; contact vaccination status; flight duration; proximity to the index case(s); contact age.

Results
11,307 index cases linked to 667,849 contacts with 5,289 secondary cases reported. In-flight SAR was 0.79% (95% CI: 0.77–0.81). Increasing numbers of symptomatic cases (when > 4 index cases compared to one index case aOR 1.85; 95% CI: 1.40–2.44) and seating proximity to an index case (seated within compared to outside of two rows OR 1.82; 95% CI: 1.50–2.22) were associated with increased risk of secondary cases. Full vaccination history was protective (aOR 0.52; 95% CI: 0.47–0.57).

Conclusions
This study confirms that in-flight transmission of SARS-CoV-2 occurred. There are factors associated with increased risk of infection. Contact tracing identified exposed persons who subsequently developed infection. A targeted approach to contact tracing passengers with the highest exposure risk could be an effective use of limited public health resources.
Drug-resistant profiles of extracellular vesicles predict therapeutic response in TNBC patients receiving neoadjuvant chemotherapy
Authors: Kim Min Woo , Lee Hyojung , Lee Suji , Moon Sol , Kim Young , Kim Joon Ye , Kim Seung Il , Kim Jee Ye 
Published: February 2024

Abstract
Background
Predicting tumor responses to neoadjuvant chemotherapy (NAC) is critical for evaluating prognosis and designing treatment strategies for patients with breast cancer; however, there are no reliable biomarkers that can effectively assess tumor responses. Therefore, we aimed to evaluate the clinical feasibility of using extracellular vesicles (EVs) to predict tumor response after NAC.

Methods
Drug-resistant triple-negative breast cancer (TNBC) cell lines were successfully established, which developed specific morphologies and rapidly growing features. To detect resistance to chemotherapeutic drugs, EVs were isolated from cultured cells and plasma samples collected post-NAC from 36 patients with breast cancer.

Results
Among the differentially expressed gene profiles between parental and drug-resistant cell lines, drug efflux transporters such as MDR1, MRP1, and BCRP were highly expressed in resistant cell lines. Drug efflux transporters have been identified not only in cell lines but also in EVs released from parental cells using immunoaffinity-based EV isolation. The expression of drug resistance markers in EVs was relatively high in patients with residual disease compared to those with a pathological complete response.

Conclusions
The optimal combination of drug-resistant EV markers was significantly efficient in predicting resistance to NAC with 81.82% sensitivity and 92.86% specificity.