bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activity settings as the unit of analysis: A theoretical basis for community intervention and development
Authors: O'Donnell Clifford R. , Tharp Roland G. , Wilson Kathleen 
Published: January 2005

AbstractTheory, methods, and action develop within context, one of which is the intellectual climate of an era. Community psychology is directly and indirectly interactive with many intellectual currents, such as postmodernism, semiotics, hermeneutics, and dialogism. These ideas are discussed as they impact on community psychology, with an emphasis on the reemergence of meaning as a central condition of psychology and community. Meaning is of key importance to the unifying concept in the transactional theoretical model which is presented. How this model might serve as a conceptual framework for an asset approach to community intervention and development is discussed. Increasingly, it is recognized that the development of theory to guide community research and action is necessary to advance intervention and to realize the potential of community psychology. It is argued that community psychology is positioned by concepts and practice squarely at the point of emerging thought, and can make fuondational contributions to general social science. The next article in this special section illustrates concepts of this model in an early education program and the remaining article illustrates the influence of ecocultural factors.
Early education as community intervention: Assisting an ethnic minority to be ready for school
Authors: Roberts Richard N. 
Published: January 2005

AbstractAn early education program designed for an ethnic minority of Native Hawaiians implemented at the community rather than the individual child level of intervention is described. The program development effort is theory driven and provides an opportunity to investigate the application of neo‐Vygotskian theory to an applied community educational problem. Though not specifically derived from the work of O'Donnell, Tharp, and Wilson (1993), the project reported here shares a common intellectual heritage. This paper demonstrates the parallels between the early education program developed for Hawaiian children and the applicability of the theory presented in the work of O'Donnell et al.
Methodological issues in prevention research: An introduction to the special issue
Authors: Jansen Mary A. , Johnson Elaine M. 
Published: January 2005

AbstractIn recent years increased attention has been directed to prevention research as a means of solving the multitude of complex social and health problems which confront individuals and their societies. Abuse of substances, behavioral dysfunctions, violence, emotional disorders, educational failures, unwanted pregnancies and sexually transmitted diseases are but a few examples of these most difficult problems. As our recognition of the need for sound prevention strategies has grown, so has our sophistication in designing prevention research studies to evaluate the effectiveness of these new strategies. And that sophistication has brought new challenges, some of which are capable of stifling progress because of their complexity. This problem is certain to grow in importance as interventions with multiple components are used more frequently to meet the challenges of the complex social and health problems we face. This special issue evolved out of the recognition that prevention researchers are confronted by a myriad of difficult methodological issues which have inhibited progress in this area. The articles in the issue present innovative methodological solutions designed to overcome these problems so the field can move forward.
Preventing problems in prevention research
Authors: Sechrest Lee B. 
Published: January 2005

AbstractThe design, conduct, and analysis of prevention research efforts present formidable challenges, but as the papers in this volume illustrate, the problems of prevention research are probably not altogether intractable; they simply require the best of our thinking and the firmest of our commitments. The papers included in this issue represent some of the best thinking likely to be available, and, in aggregate, they give reason for some optimism about prevention research. Which is fortunate, because it is by now abundantly clear that treatment of all the assorted personal and social maladies that afflict us individually and as a society, is impossibly intrusive and expensive, even if we were certain we knew what to do, and we are not. Treatment research is only a step or two ahead of prevention research in nearly any field.
Peer telephone dyads: Putting conclusions on hold
Authors: Gottlieb Benjamin H. 
Published: January 2005

ConclusionThrough systematic study of intervention trials, which are as carefully crafted and as critically examined as the one reported here, we can gain new insights into ways of intentionally creating the interpersonal and environmental conditions conducive to the expression and acceptance of support. To plan appropriate and acceptable changes in the social ecology, we must develop a theory of the intervention, drawing on both the voluminous generative base of research on social support and on knowledge gained from examination of the particular forces that potentiate and limit exchanges of support in the networks of the intended beneficiaries. In conducting the intervention, careful recordings of the interpersonal transactions that take place and their bearing on the parties' perceptions of the evolving character of the relationship can bring the needed process dimension to emergent theory on social support. And by evaluating the impact of the intervention on a range of proximal and distal outcomes, we can discern more clearly how social support's mechanisms of action confer measurable benefits, particularly those prized by the participants themselves.
Letter to a genetic counselor
Authors: Green Rose 
Published: January 2005

AbstractThe author describes her personal experience terminating a pregnancy after receiving an abnormal amniocentesis result: While still waiting for the results, she and her husband attempted to deny the importance of the pregnancy, an approach which they subsequently regretted. When they received the abnormal result, they found themselves able to make necessary decisions quickly, despite being in shock. It then took some time before they realized what a major loss they had actually suffered. The painful aftermath included accepting emotional responsibility for their decision to abort, as well as explaining that difficult decision to their older children. Thoughts of a possible subsequent pregnancy could not be faced at once. Over time, the author found the support of friends and colleagues, and even of a support group, to be invaluable. All in all, the entire experience was more painful than she would have predicted, and she hopes that this account will prove helpful both to genetic counselors and to other patients who receive abnormal results from prenatal diagnosis. A list of suggested readings is appended.
The future of genetics in public health
Authors: Meaney F. John 
Published: January 2005

AbstractDuring the 1980s, we have witnessed an erosion in federal and state funding for the coordination of genetic services in state health departments. As this decade begins, the federal budget for the support of the national genetics program is less than half of the total available in 1980. In addition, priorities in state maternal and child health programs have changed, and genetic services have often received lower rankings aside of programs to improve prenatal care and to address problems such as teenage pregnancy. Clearly we are at a critical point for the future of genetics programs in the public health arena. Fortunately, despite the problems with funding and priority setting, important coalitions have been built during the past decade and continued advances in medical genetics have made it difficult for public health officials to ignore the potential impact of genetic services. Council of Regional Networks for Genetic Services (CORN) and the regional genetic services networks have provided opportunities for interaction and coordination between genetic services providers and public health officials that had not been available to any significant extent. The full potential of these coalitions has yet to be realized, but efforts in data collection, development of national guidelines and standards, and information sharing and networking have already had an influence on state genetic services programs. Continuing research on the genetics of chronic diseases and the influence of discoveries in molecular genetics will undoubtedly broaden the current scope of state public health programs in genetics, which all too often have been limited to newborn screening and administration of grants for genetic services. The regional and national genetic services programs should be strengthened and appropriate training programs should be developed in Schools of Public Health to provide the expertise and leadership that will be needed to guide the future of genetics in public health.
Ethical principles and cultural integrity in health care delivery: Asian ethnocultural perspectives in genetic services
Authors: Wang Vivian , Marsh Frank H. 
Published: January 2005

AbstractThe tradition of Western thought is not always ubiquitous when working within the context of different ethnocultural populations, particularly that of Asian culture, and often poses ethical quandaries for health care professionals engaged in genetic services. For example, understanding the Asian ethnocultural perspective of patient autonomy, informed consent, and nondirective counseling from the Western perspective may be inappropriate and compromise cultural integrity and the delivery of effective genetic services because: (1) A distinction exists between the role of Asian (collective) autonomy vs. Western (individual) autonomy in the decision‐making process. (2) The medical model is complementary to Asian expectations and respect of authority and may result in an uninformed consent to genetic professional's recommendations. (3) Misinterpretations of the normative decision‐making process, misconceptions of normalcy, and the limits of tolerance of nondirective counseling may result in less effective treatment and compliance. Therefore, we suggest that acknowledging the impact of ethnicity and culture on ethical principles as applied in genetic services is essential in the assessment and delivery of ethically and culturally appropriate health care.
Medium‐chain acyl CoA dehydrogenase deficiency: Its relationship to SIDS and the impact on genetic counseling
Authors: McConkie‐Rosell Allyn , Iafolla A. Kimberly 
Published: January 2005

AbstractSudden infant death syndrome (SIDS) is defined as the sudden and unexpected death of an apparently healthy infant under 1 year of age. Routine autopsies often provide few clues as to the cause of death and rarely include a biochemical evaluation. Genetic counseling for SIDS can be difficult as recurrence risks vary depending on the age at death and the number of deaths which have occurred in the family. Biochemical disorders may account for up to 5% of SIDS. Of the metabolic disorders known to be involved in SIDS, the most commonly found is medium‐chain acyl CoA dehydrogenase deficiency (MCAD). MCAD is an autosomal recessive disorder of fatty acid oxidation which accounts for up to 1% of SIDS. For some families, the addition of a postmortem biochemical investigation can identify an unsuspected metabolic disorder as the cause of death. Once the diagnosis is established, accurate genetic counseling can then be provided. Metabolic testing of the surviving siblings of victims of sudden death, and the subsequent identification of those due to MCAD can prevent the tragedy of recurrent SIDS in some families. In addition, screening the survivors of an acute life threatening event (ALTE) may also prevent a recurrence.
The challenge of “Healthy People 2000”
Authors: Crocker Allen C. 
Published: January 2005

AbstractThe federal Office of Disease Prevention and Health Promotion (U.S. Public Health Service) has again prepared a compilation of goals and objectives for the health of the Nation's people, applicable to anticipated achievements within the decade ending in the year 2000. This effort involved work groups from numerous federal agencies, testimony in regional hearings from 800 citizens, and enrollment of a consortium of 300 national organizations. The final product, “Healthy People 2000,” was released in September 1990, and contains 298 measurable objectives. Content regarding children, genetic disorders, developmental disabilities, and disabilities in general is less featured and less specific than was hoped for. Ten objectives of relatively direct concern to geneticists are discussed, plus 23 others of interest in the maternal and child health field. On balance, it is suggested that the Healthy People 2000 objectives can be useful and stimulating for workers in genetics, child health care, and disabilities' services. Regrettably, there was no simultaneous proposal of legislation that would provide new federal programs, funding, or assistance to states.
Genetic counseling for deaf adults: Communication/language and cultural considerations
Authors: Israel Jamie , Cunningham Margaret , Thumann Helen , Arnos Kathleen Shaver 
Published: January 2005

AbstractIt has been estimated that at least 50% of congenital or early onset deafness loss has a genetic etiology. Genetic services have traditionally been utilized by hearing parents of deaf children. Deaf adults could also greatly benefit from genetic counseling services. However, many deaf adults do not seek genetic services due in part to the communication/language and cultural differences of this group. Deaf people communicate in various ways including the use of sign language, oral communication, writing, or a combination of these modes. Also, while some deaf individuals are part of the hearing culture, others are part of the Deaf culture which has its own language, values, and traditions. Culturally Deaf individuals do not see themselves as handicapped or disabled. The genetic professional's awareness of the communication/language and cultural needs of this group, as well as their agency's responsibilities under section 504 of the Rehabilitation Act of 1973, may increase the accessibility of genetic services and contribute to the provision of successful genetic counseling for deaf adults.
Continuing education in genetics for Adoption workers in Wisconsin
Authors: Burns Joan K. , Reiser Catherine A. 
Published: January 2005

AbstractIn response to 1982 legislation and 1984 changes in the Wisconsin Adoption Records Laws requiring the collection of a medical‐genetic history at the time of termination of parental rights, a continuing education program in genetics was conducted for adoption workers in 1984 and 1985. The education program provided 14 workshops in five locations throughout the state and consisted of a variety of formats and levels of training. In all, 164 participants were trained at least at the introductory level with approximately 40 of these individuals taking part in the advanced levels of training. Evaluations of the training by participants and by a sample of the agency supervisors of trainees ranked the program very highly. A review of genetic history forms completed in post‐training sessions verified the expectation that training was beneficial. Trained workers completing the medical‐genetic history forms scored somewhat higher than untrained workers and much higher than parents who completed the forms without professional guidance. Medical‐genetic history forms completed by birth fathers as part of step‐parent adoptions contained little to no useful information about the birth father's genetic background.
Genetics and adoption: Forming a partnership
Authors: Delp Kathleen J. , Kaepernick Lisa A. 
Published: January 2005

AbstractGenetic issues are demanding more attention in the area of public health. Adoption agencies and policymakers are beginning to address these issues where they relate to the adoption process and to the many families involved in adoption in this country. Genetic counselors need to play an active role as both educators of and consultants for adoption professionals and the families with whom they work. To facilitate a partnership between genetics and adoption we have developed a workshop intended to educate adoption professionals about the lifelong implications of genetic conditions on the adoption triad.
Transfer of genetic information in an organism
Authors: Kordyum V.A. , Shpylova S.P. , Andrienko V.I. , Sukhorada O.M. , Ruban T.A. , Deryabina O.G. 
Published: January 2005

AbstractNew knowledge in biology led us to a better understanding of organization and functioning of living organisms. Today, re‐evaluation of our concept of human biology is taking place. Theoretical analysis shows that taking into account the complexity of the organism and frequency of spontaneous mutations, it is impossible to explain the real time of organismal life. Therefore, besides extant systems, other repair systems must also exist. There are three “levels” at which a cell population withstands mutational pressure. First—intracellular (repair), second—intercellular (all forms of informational flows), and third—cellular replacement. Stem cells undertake regenerative functions following damage at the level of the tissue. They are also influenced by mutations, and for stem cells, it is most important that they preserve and support their full activity.
X-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster
Authors: ELKINS Jonathan M. , KERSHAW Nadia J. , SCHOFIELD Christopher J. 
Published: January 2005

The orf6 gene from the clavulanic acid biosynthesis gene cluster encodes an OAT (ornithine acetyltransferase). Similar to other OATs the enzyme has been shown to catalyse the reversible transfer of an acetyl group from N-acetylornithine to glutamate. OATs are Ntn (N-terminal nucleophile) enzymes, but are distinct from the better-characterized Ntn hydrolase enzymes as they catalyse acetyl transfer rather than a hydrolysis reaction. In the present study, we describe the X-ray crystal structure of the OAT, corresponding to the orf6 gene product, to 2.8 Å (1 Å=0.1 nm) resolution. The larger domain of the structure consists of an αββα sandwich as in the structures of Ntn hydrolase enzymes. However, differences in the connectivity reveal that OATs belong to a structural family different from that of other structurally characterized Ntn enzymes, with one exception: unexpectedly, the αββα sandwich of ORF6 (where ORF stands for open reading frame) displays the same fold as an DmpA (L-aminopeptidase D-ala-esterase/amidase from Ochrobactrum anthropi), and so the OATs and DmpA form a new structural subfamily of Ntn enzymes. The structure reveals an α2β2-heterotetrameric oligomerization state in which the intermolecular interface partly defines the active site. Models of the enzyme–substrate complexes suggest a probable oxyanion stabilization mechanism as well as providing insight into how the enzyme binds its two differently charged substrates.
The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex
Authors: BEITES Crestina L. , CAMPBELL Kristen A. , TRIMBLE William S. 
Published: January 2005

SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins are supposed to mediate the docking and/or fusion of the vesicle with the plasma membrane. However, it is not clearly understood how this process is regulated. In a search for potential SNARE regulators, we recently identified septin 5 (Sept5) as a novel SNARE interacting protein. Septins were first identified as filamentous proteins required for cytokinesis in yeast. Several septins have now been identified in mammals but little is known about their functions. We have previously shown that Sept5 is predominantly expressed in the brain, where it associates with vesicles and membranes through its interaction with the SNARE domain of syntaxin 1A. Furthermore, Sept5 appears to inhibit exocytosis, possibly by regulating vesicle targeting and/or fusion events. To gain insight into the role of Sept5, we have mapped the Sept5 domains important for syntaxin binding. We also investigated the ability of Sept5 to bind to syntaxin when in various protein complexes. Although Sept5 cannot bind an nSec1–syntaxin complex, it can bind syntaxin in a SNARE complex. This interaction is occluded by the binding of α-SNAP, suggesting that Sept5 may regulate the availability of SNARE proteins through its interaction with syntaxin and the 7 S complex.
Identification and characterization of pleckstrin-homology-domain-dependent and isoenzyme-specific Akt inhibitors
Authors: BARNETT Stanley F. , DEFEO-JONES Deborah , FU Sheng , HANCOCK Paula J. , HASKELL Kathleen M. , JONES Raymond E. , KAHANA Jason A. , KRAL Astrid M. , LEANDER Karen , LEE Ling L. , MALINOWSKI John , McAVOY Elizabeth M. , NAHAS Debbie D. , ROBINSON Ronald G. , HUBER Hans E. 
Published: January 2005

We developed a high-throughput HTRF (homogeneous time-resolved fluorescence) assay for Akt kinase activity and screened approx. 270000 compounds for their ability to inhibit the three isoforms of Akt. Two Akt inhibitors were identified that exhibited isoenzyme specificity. The first compound (Akt-I-1) inhibited only Akt1 (IC50 4.6 μM) while the second compound (Akt-I-1,2) inhibited both Akt1 and Akt2 with IC50 values of 2.7 and 21 μM respectively. Neither compound inhibited Akt3 nor mutants lacking the PH (pleckstrin homology) domain at concentrations up to 250 μM. These compounds were reversible inhibitors, and exhibited a linear mixed-type inhibition against ATP and peptide substrate. In addition to inhibiting kinase activity of individual Akt isoforms, both inhibitors blocked the phosphorylation and activation of the corresponding Akt isoforms by PDK1 (phosphoinositide-dependent kinase 1). A model is proposed in which these inhibitors bind to a site formed only in the presence of the PH domain. Binding of the inhibitor is postulated to promote the formation of an inactive conformation. In support of this model, antibodies to the Akt PH domain or hinge region blocked the inhibition of Akt by Akt-I-1 and Akt-I-1,2. These inhibitors were found to be cell-active and to block phosphorylation of Akt at Thr308 and Ser473, reduce the levels of active Akt in cells, block the phosphorylation of known Akt substrates and promote TRAIL (tumour-necrosis-factor-related apoptosis-inducing ligand)-induced apoptosis in LNCap prostate cancer cells.