bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the Complete Description Of Entangled Systems Part I–Exploring Hidden Variables and Context Communication Cost in Simulating Quantum Correlations
Authors: Svozil Karl 
Published: January 2024

AbstractIn this article, we investigate classical simulations of quantum-type probabilities and correlations that defy Boole’s conditions of possible experience, specifically the Clauser-Horne-Shimony-Holt inequality, and even surpass the Tsirelson bound. We demonstrate that such violations can be achieved by encoding a single bit to communicate the measurement context. Our findings highlight the crucial role of context communication in reproducing quantum correlations that are stronger than classical limits, providing insights into the fundamental principles underlying entangled systems.
dCas9/CRISPR-based methylation of O-6-methylguanine-DNA methyltransferase enhances chemosensitivity to temozolomide in malignant glioma
Authors: Zapanta Rinonos Serendipity , Li Tie , Pianka Sean Thomas , Prins Terry J. , Eldred Blaine S. C. , Kevan Bryan M. , Liau Linda M. , Nghiemphu Phioanh Leia , Cloughesy Timothy F. , Lai Albert 
Published: January 2024

Abstract
Background
Malignant glioma carries a poor prognosis despite current therapeutic modalities. Standard of care therapy consists of surgical resection, fractionated radiotherapy concurrently administered with temozolomide (TMZ), a DNA-alkylating chemotherapeutic agent, followed by adjuvant TMZ. O-6-methylguanine-DNA methyltransferase (MGMT), a DNA repair enzyme, removes alkylated lesions from tumor DNA, thereby promoting chemoresistance. MGMT promoter methylation status predicts responsiveness to TMZ; patients harboring unmethylated MGMT (~60% of glioblastoma) have a poorer prognosis with limited treatment benefits from TMZ.

Methods
Via lentiviral-mediated delivery into LN18 glioma cells, we employed deactivated Cas9-CRISPR technology to target the MGMT promoter and enhancer regions for methylation, as mediated by the catalytic domain of the methylation enzyme DNMT3A. Methylation patterns were examined at a clonal level in regions containing Differentially Methylation Regions (DMR1, DMR2) and the Methylation Specific PCR (MSP) region used for clinical assessment of MGMT methylation status. Correlative studies of genomic and transcriptomic effects of dCas9/CRISPR-based methylation were performed via Illumina 850K methylation array platform and bulk RNA-Seq analysis.

Results
We used the dCas9/DNMT3A catalytic domain to achieve targeted MGMT methylation at specific CpG clusters in the vicinity of promoter, enhancer, DMRs and MSP regions. Consequently, we observed MGMT downregulation and enhanced glioma chemosensitivity in survival assays in vitro, with minimal off-target effects.

Conclusion
dCas9/CRISPR is a viable method of epigenetic editing, using the DNMT3A catalytic domain. This study provides initial proof-of-principle for CRISPR technology applications in malignant glioma, laying groundwork for subsequent translational studies, with implications for future epigenetic editing-based clinical applications.
Key stakeholder voices: Investigating student perceptions of teachers’ use of assessment for learning
Authors: Wolterinck-Broekhuis Christel H. D. , Poortman Cindy L. , Schildkamp Kim , Visscher Adrie J. 
Published: January 2024

AbstractMany schools aim to implement Assessment for Learning (AfL) to stimulate students to take more ownership of their learning and develop self-regulatory skills. This survey-based study is among the few in the field showing how students experience the extent of implementation of AfL, here in English language and mathematics classes in 12 Dutch secondary schools. Analysis showed no differences between the subjects. Students (N = 685) experienced activities to clarify the learning intentions and success criteria and eliciting evidence on their learning progress regularly (between 50–74% of the lessons). Students hardly ever experienced activities aimed at peer- and self-assessment (less than 25% of the lessons). However, cluster analysis revealed three distinct clusters related to the extent of AfL strategy use students experienced. Overall, we can conclude that AfL is not yet fully integrated into teaching practices. Teachers’ skills, knowledge and attitudes required to increase student engagement in AfL practices and strengthen students’ self-regulated learning need more attention in future teacher professional development trajectories.
High power tunable Raman fiber laser at 1.2 μm waveband
Authors: Zhang Yang , Xu Jiangming , Liang Junrui , Ye Jun , Li Sicheng , Ma Xiaoya , Pan Zhiyong , Leng Jinyong , Zhou Pu 
Published: January 2024

AbstractDevelopment of a high power fiber laser at special waveband, which is difficult to achieve by conventional rare-earth-doped fibers, is a significant challenge. One of the most common methods for achieving lasing at special wavelength is Raman conversion. Phosphorus-doped fiber (PDF), due to the phosphorus-related large frequency shift Raman peak at 40 THz, is a great choice for large frequency shift Raman conversion. Here, by adopting 150 m large mode area triple-clad PDF as Raman gain medium, and a novel wavelength-selective feedback mechanism to suppress the silica-related Raman emission, we build a high power cladding-pumped Raman fiber laser at 1.2 μm waveband. A Raman signal with power up to 735.8 W at 1252.7 nm is obtained. To the best of our knowledge, this is the highest output power ever reported for fiber lasers at 1.2 μm waveband. Moreover, by tuning the wavelength of the pump source, a tunable Raman output of more than 450 W over a wavelength range of 1240.6–1252.7 nm is demonstrated. This work proves PDF’s advantage in high power large frequency shift Raman conversion with a cladding pump scheme, thus providing a good solution for a high power laser source at special waveband.
Graphical Abstract
The Study of Antistaphylococcal Potential of Omiganan and Retro-Omiganan Under Flow Conditions
Authors: Jaśkiewicz Maciej , Neubauer Damian , Sikora Karol , Bauer Marta , Bartoszewska Sylwia , Błażewicz Izabela , Marek Dariusz , Barańska-Rybak Wioletta , Kamysz Wojciech 
Published: January 2024

AbstractStaphylococcus aureus is considered one of the leading pathogens responsible for infections in humans and animals. The heterogeneous nature of diseases caused by these bacteria is due to the occurrence of multiple strains, differentiated by several mechanisms of antibiotic resistance and virulence factors. One of these is the ability to form biofilm. Biofilm-associated bacteria exhibit a different phenotype that protects them from external factors such as the activity of immune system or antimicrobial substances. Moreover, it has been shown that the majority of persistent and recurrent infections are associated with the presence of the biofilm. Omiganan, an analog of indolicidin - antimicrobial peptide (AMP) derived from bovine neutrophil granules, was found to exhibit high antistaphylococcal and antibiofilm potential. Furthermore, its analog with a reversed sequence (retro-omiganan) was found to display enhanced activity against a variety of pathogens. Based on experience of our group, we found out that counterion exchange can improve the antistaphylococcal activity of AMPs. The aim of this study was to investigate the activity of both compounds against S. aureus biofilm under flow conditions. The advantage of this approach was that it offered the opportunity to form and characterize the biofilm under more controlled conditions. To do this, unique flow cells made of polydimethylsiloxane (PDMS) were developed. The activity against pre-formed biofilm as well as AMPs-treated bacteria was measured. Also, the incorporation of omiganan and retro-omiganan into the channels was conducted to learn whether or not it would inhibit the development of biofilm. The results of the microbiological tests ultimately confirmed the high potential of the omiganan and its retro-analog as well as the importance of counterion exchange in terms of antimicrobial examination. We found out that retro-omiganan trifluoroacetate had the highest biofilm inhibitory properties, however, acetates of both compounds exhibited the highest activity against planktonic and biofilm cultures. Moreover, the developed methodology of investigation under flow conditions allows the implementation of the studies under flow conditions to other compounds.
Beyond Collaborative Learning: a Comparison of Small Groups in Face-to-Face and Online Settings
Authors: Song Xiaomei , Elftman Michael 
Published: January 2024

AbstractSmall group-based instructional approaches such as case-based learning (CBL) and team-based learning (TBL) are widely used in medical education to promote collaboration and team learning. During the pandemic, many medical schools shifted from face-to-face instruction to online settings. While CBL/TBL are intended to foster collaborative skills, it is unclear how its use evolves in an online setting and whether the online setting impacts students’ perceptions and behaviors in collaboration. This study examined how the change from in-person to online CBL/TBL impacted students’ collaboration. We used a mixed-methods sequential design, first collecting and analyzing retrospective cohort quantitative data with the Class of 2023 through peer evaluation surveys followed by six focus groups. Students’ assessment of their peers was generally positive. Nonparametric testing showed significant differences for two questions with less positive perceptions in the virtual setting compared to when students had in-person CBL/TBL. The focus group results identified several themes related to collaboration and learning communities. In the virtual setting, students not only lost collaboration opportunities with their group members in CBL/TBL, but also learning opportunities and social connections with other groups and the community as a whole. Virtual learning environments may have presented challenges for collaborative learning and the establishment of a sense of community.
Discussion of Josh Milburn’s Just Fodder: The Ethics of Feeding Animals
Authors: Pepper Angie 
Published: January 2024

AbstractIn Just Fodder: The Ethics of Feeding Animals, Josh Milburn thinks through the implications of feeding animals by focusing on the relationships between humans and three different groups of animals: (1) animal companions; (2) animal neighbours; and (3) wild animals. In my comments, I concentrate on how the actions and agency interests of these animals problematise some of Milburn’s assumptions and normative prescriptions. My overall aim is to show how giving animal agency more prominence in our thinking about what we owe to them has significant implications. It is my view that current theorising about other animals focuses too heavily on their experiential welfare and not enough on what animals do and the normative significance of what they do. Though I agree with Milburn that all animals have a right against being killed and made to suffer, a full ethical analysis of our relations with other animals must also consider their (equally weighty) interest in self-determination.
Continuous flow synthesis of meso-substituted porphyrins with inline UV–Vis analysis
Authors: Parveen Firdaus , Morris Henry J. , West Harvey , Slater Anna G. 
Published: January 2024

AbstractPorphyrin derivatives have found diverse applications due to their attractive photophysical and catalytic properties, but remain challenging to synthesize, particularly at scale. Porphyrin synthesis thus stands to benefit from the more controlled environment, opportunities for efficient optimization, and potential for scale-up available in flow. Here, we have transferred Lindsey porphyrin synthesis into flow, enabling controlled timing for oxidation and neutralization steps and real time monitoring of the reaction mixture with inline UV–Vis analysis. For tetraphenyl porphyrin (TPP), inline UV–Vis showed the presence of protonated TPP, formed due to residual acid. Thus, inline monitoring allowed optimization of the neutralization step to improve yield. Three further porphyrin substrates were produced in flow; in two cases, the yield from inline UV was significantly higher than the yield from post-purification, identifying further yield losses that could be recovered by modifying the purification step. The workflow presented here can be adapted to multiple substrates to systematically optimise porphyrin yield, reducing the time needed to develop scalable routes to these valuable compounds.
Biochar reduced the mineralization of native and added soil organic carbon: evidence of negative priming and enhanced microbial carbon use efficiency
Authors: Kalu Subin , Seppänen Aino , Mganga Kevin Z. , Sietiö Outi-Maaria , Glaser Bruno , Karhu Kristiina 
Published: January 2024

AbstractBiochar has been widely recognized for its potential to increase carbon (C) sequestration and mitigate climate change. This potential is affected by how biochar interacts with native soil organic carbon (SOC) and fresh organic substrates added to soil. However, only a few studies have been conducted to understand this interaction. To fill this knowledge gap, we conducted a 13C-glucose labelling soil incubation for 6 months using fine-textured agricultural soil (Stagnosol) with two different biochar amounts. Biochar addition reduced the mineralization of SOC and 13C-glucose and increased soil microbial biomass carbon (MBC) and microbial carbon use efficiency (CUE). The effects were found to be additive i.e., higher biochar application rate resulted in lower mineralization of SOC and 13C-glucose. Additionally, soil density fractionation after 6 months revealed that most of the added biochar particles were recovered in free particulate organic matter (POM) fraction. Biochar also increased the retention of 13C in free POM fraction, indicating that added 13C-glucose was preserved within the biochar particles. The measurement of 13C from the total amino sugar fraction extracted from the biochar particles suggested that biochar increased the microbial uptake of added 13C-glucose and after they died, the dead microbial residues (necromass) accumulated inside biochar pores. Biochar also increased the proportion of occluded POM, demonstrating that increased soil occlusion following biochar addition reduced SOC mineralization. Overall, the study demonstrates the additional C sequestering potential of biochar by inducing negative priming of native SOC as well as increasing CUE, resulting in the formation and stabilization of microbial necromass.
Graphical Abstract
Synergistically enhancing nitrate reduction into N2 in water by N-doped Pd–Cu biochar bimetallic single-atom electrocatalysis
Authors: Zhang Lihao , Wu Yuqing , Zhu Zongqiang , Zhu Yinian , Dong Yi , Liang Meina , Deng Huan 
Published: January 2024

AbstractNoble metal materials have been identified as high efficiency catalysts for electrocatalytic reduction of nitrate, and the synthesis and manufacture of high catalytic activity and environmentally friendly catalysts of activating hydrogen for water purification applications is extremely attractive. In this work, the Pd–Cu single-atom catalysts (Pd–Cu-N-BC) were first prepared by direct growth of Pd–Cu single-atom on bamboo biochar by regulating the concentration of precursors and doping method, and then enhanced electrocatalytic reduction nitrate performance and N2 generation. The results showed that Pd–Cu-N-BC displayed excellent catalytic activity and reusability in electrocatalytic reduction nitrate with a low potential of 0.47 V vs. RHE (@10 mA cm−2). The maximum nitrate removal efficiency and N2 generation could reach about 100% and 72.32% within 180 min, respectively. The density functional theory (DFT) calculations confirmed that Cu atoms could catalyze the electrochemical reduction of nitrate to nitrite, and Pd atoms anchored in the nitrogen-doped biochar (N-BC) lattice could catalyze electrochemical reduction of nitrite to N2 involving the formation of hydrogen radical (H*). The characterization results of XANES showed that electronic synergistic effect between Pd and Cu single atoms significantly promotes the N2 production through hydrogenation while inhibiting the generation of byproducts, leading to significantly enhanced electrocatalytic reduction of nitrate to N2. Finally, Pd–Cu-N-BC was designed as a 3D particle electrode for enhanced electrocatalytic reduction of nitrate, exhibiting excellent stability and reusability, which could be considered as a suitable candidate for applications in the remediation of nitrate contamination.
Graphical Abstract
A design perspective on how to tackle gender biases when developing AI-driven systems
Authors: González Ana Santana , Rampino Lucia 
Published: January 2024

Abstract
A growing awareness of bias in artificial intelligence (AI) systems has recently emerged, leading to an increased number of publications discussing ethics in AI. Nevertheless, the specific issue of gender bias remains under-discussed. How can design contribute to preventing the emergence of gender bias in AI-driven systems? To answer this question, we investigated the current state of AI ethical guidelines within the European Union. The results revealed that most guidelines do not acknowledge gender bias but address discrimination. This raised our concerns, as addressing multiple biases simultaneously might not effectively mitigate any of them due to their often-unconscious nature. Furthermore, our results revealed a lack of quantitative evidence supporting the effectiveness of bias prevention implementation methods and solutions. In conclusion, based on our analysis, we propose four recommendations for designing effective guidelines to tackle gender biases in AI. Moreover, we stress the central role of diversity in embedding the gender perspective from the beginning in any design activity.
Anesthetic drug discovery with computer-aided drug design and machine learning
Authors: Liu Xianggen , Xue Zhe , Luo Mingmin , Ke Bowen , Lv Jiancheng 
Published: January 2024

AbstractComputer-aided drug design (CADD) has emerged as a highly effective and indispensable tool for streamlining the drug discovery process, leading to significant reductions in cost and time. The integration of CADD with machine learning (ML) and deep learning (DL) technologies further enhances its potential and promises novel advancements in the field. In this article, we provide a review of the computational methods employed in the development of novel anesthetics, outlining their respective advantages and limitations. These techniques have demonstrated their utility across various stages of drug discovery, encompassing the exploration of target-ligand interactions, identification and validation of new binding sites, de novo drug design, evaluation and optimization of absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties in lead compounds, as well as prediction of adverse effects. Through an in-depth exploration of computational approaches and their applications, this article aims to help relevant researchers develop safer and more effective anesthetic drugs.
Exploring the impact of climate change on long-term and short-term variability of rainfall in Madhya Pradesh, Central India
Authors: Kumar Parvendra , Sharma Vikram , Jayal Tripti , Deswal Sanjay 
Published: January 2024

AbstractThe present study assesses trends in rainfall in Madhya Pradesh, Central India, over the long (1871–2016) as well as short (1987–2016) temporal scales. The region is home to disadvantageous groups of the population including vulnerable tribals who depend on rainfall-based agriculture and forest products. Therefore, any disruption in rainfall trends due to climate change needs to be documented for better planning. This study focuses on two meteorological subdivisions, East and West Madhya Pradesh, which receive a major portion of rainfall through the Bay of Bengal branch and the Arabian Sea branch of the Indian Summer Monsoon. The study is based on the rainfall series provided by the Indian Institute of Tropical Meteorology, Pune, India. This study used the Mann–Kendall test to detect a trend and Sen’s Slope to estimate the magnitude of change in rainfall. Statistically significant negative trends were detected in total annual (− 1.125 mm y − 1) and monsoon (− 1.242 mm y − 1) rainfall over long-term observation in East Madhya Pradesh only. However, during the last 30 years, no trends have been recorded in East Madhya Pradesh. In West Madhya Pradesh, rainfall has not recorded any trend for long- as well as short-term observation periods. The non-availability of a trend in rainfall during May and October at both meteorological subdivisions shows that there is no shift in the duration of the monsoon season over long as well as short temporal observations. The decreasing rainfall trends in Eastern Madhya Pradesh over the long-term observation seem to be caused by changing patterns of tropical cyclone frequency in the Bay of Bengal.
The Private as a Core Part of International Law: The School of Salamanca, Slavery, and Marriage (Sixteenth Century)
Authors: Martineau Anne-Charlotte 
Published: January 2024

In “Gender and the Lost Private Side of International Law,” Karen Knop argued that “recuperating private international law as a lost side of international law can open up counter-disciplinary research on gender in the history of international law.”1 In this essay, I use Knop's argument to revisit our understanding of the sixteen century “School of Salamanca”2 and its importance for international legal history from a gender perspective. I focus on the practice of jurists and theologians associated with the School of Salamanca in assessing the validity of marriages of newly converted Indigenous peoples in Brazil (negros da terra), and later the validity of remarriages of Indigenous people and enslaved Africans (negros da Guiné) who had already been married in places from which they had been forcibly removed.3 To do this, these jurists and theologians engaged in private international law (or conflict of laws) reasoning. A key question involved determining what law governed each marriage—was it ius gentium, natural law, or canon law? Examining their arguments, I argue, offers an instance of Knop's insight that recuperating private international law allows us to redress the invisibility of women in the history of international law. In my case study, not only do we better understand “how power operates through international legal concepts and institutions”4 in the private sphere of the family in the colonies, but also, and crucially, how “private international law make[s] visible the effects of colonial . . . law on gender relations and national identity at home,”5 to borrow Knop's words.
Smith theory and cyclic base change functoriality
Authors: Feng Tony 
Published: January 2024

Abstract
Lafforgue and Genestier-Lafforgue have constructed the global and (semisimplified) local Langlands correspondences for arbitrary reductive groups over function fields. We establish various properties of these correspondences regarding functoriality for cyclic base change: For

$\mathbf {Z}/p\mathbf {Z}$

-extensions of global function fields, we prove the existence of base change for mod p automorphic forms on arbitrary reductive groups. For

$\mathbf {Z}/p\mathbf {Z}$

-extensions of local function fields, we construct a base change homomorphism for the mod p Bernstein center of any reductive group. We then use this to prove existence of local base change for mod p irreducible representation along

$\mathbf {Z}/p\mathbf {Z}$

-extensions, and that Tate cohomology realizes base change descent, verifying a function field version of a conjecture of Treumann-Venkatesh.
The proofs are based on equivariant localization arguments for the moduli spaces of shtukas. They also draw upon new tools from modular representation theory, including parity sheaves and Smith-Treumann theory. In particular, we use these to establish a categorification of the base change homomorphism for mod p spherical Hecke algebras, in a joint appendix with Gus Lonergan.