bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial intelligence-driven bioenergy system: digital green innovation partner selection of bioenergy enterprises based on interval fuzzy field model
Authors: Yin Shi , Gao Zengying , Mahmood Tahir 
Published: November 2023

PurposeThe aim of this study is to (1) construct a standard framework for assessing the capability of bioenergy enterprises' digital green innovation partners; (2) quantify the choice of partners for digital green innovation by bioenergy enterprises; (3) propose based on a dual combination empowerment niche digital green innovation field model.Design/methodology/approachFuzzy set theory is combined into field theory to investigate resource complementarity. The successful application of the model to a real case illustrates how the model can be used to address the problem of digital green innovation partner selection. Finally, the standard framework and digital green innovation field model can be applied to the practical partner selection of bioenergy enterprises.FindingsDigital green innovation technology of superposition of complementarity, mutual trust and resources makes the digital green innovation knowledge from partners to biofuels in the enterprise. The index rating system included eight target layers: digital technology innovation level, bioenergy technology innovation level, bioenergy green level, aggregated digital green innovation resource level, bioenergy technology market development ability, co-operation mutual trust and cooperation aggregation degree.Originality/valueThis study helps to (1) construct the evaluation standard framework of digital green innovation capability based on the dual combination empowerment theory; (2) develop a new digital green innovation domain model for bioenergy enterprises to select digital green innovation partners; (3) assist bioenergy enterprises in implementing digital green innovation practices.
Advocacy is contagious: the PDS effect
Authors: Cárdenas Jennifer 
Published: November 2023

PurposeThis article, written by a multilingual learner program (MLP) specialist, provides a first-hand account of how a professional development school (PDS) (school–university partnership school) promotes teacher advocacy.Design/methodology/approachDue to the subject of the piece, no research methods were necessary.FindingsDue to the subject of the piece, there are no findings.Originality/valueThe teacher details the teacher's educational journey from intern to doctoral candidate.
Floral interventions enhance flower visitor communities and pollination services in moringa plantations
Authors: Dhandapani Selva , Pakkirisamy Manikandan , Rajaraman Ranjith , Garratt Michael P. D. , Potts Simon G. , Raj Rengalakshmi , Subramanian Malarvannan , Senapathi Deepa 
Published: November 2023

Abstract

Pollination is a crucial ecosystem service contributing to global food security. Moringa (Moringa oleifera) is an economically important tropical crop, mostly cultivated by smallholder farmers.

Well‐established approaches of planting flower‐rich patches used in temperate agroecosystems for pollination enhancement were adapted to tropical moringa systems. Based on existing evidence, we hypothesised that floral interventions would improve flower visitor diversity and abundance in the crop, thereby increasing pollination services and moringa yield and quality.

We used standardised methods to survey flower visitors on moringa and assess economically relevant measures of crop yield quality and quantity. We selected 24 moringa fields in Tamil Nadu, India, to compare fields with and without floral interventions. We planted red gram, Cajanus cajan as a border crop, and marigold, Tagetes erecta as an intercrop on moringa fields to enhance floral resource availability for pollinators. These interventions were co‐designed with local farmers to ensure additional benefits to their community.

We found that flower visitor abundance and species richness were significantly (50% for abundance and 33% for species richness) greater in fields with floral interventions compared with control fields. We also found that the percentage of flowers that resulted in harvestable fruits were significantly (30%) greater in fields with floral intervention. Production benefits in yield and quality were significantly positively correlated with the abundance and species richness of flower visitors.

Synthesis and applications. Our results provide clear evidence that floral interventions in the form of intercropping and border cropping can enhance pollinator communities and services they provide in tropical smallholder systems. These findings underpin a practical management option for farmers to enhance flower visitor communities and pollination services, which can potentially also provide additional co‐benefits to farmers, improving livelihoods and sustainable production.
Hypoglycemic and hypolipidemic properties of oil derived from Agrius convolvuli
Authors: Zhang Yuhan , Xue Hanling , Cheng Yuanxia , Wu Shengjun 
Published: November 2023

AbstractThis study aims to determine the hypoglycemic and hypolipidemic properties of oil derived from Agrius convolvuli (Lepidoptera: Sphingidae) in KKAy mice, a cross between diabetic (KK) and lethal yellow (Ay) mice. A group of 12 KKAy mice were fed with AIN‐76 feed containing oil derived from A. convolvuli pupae (ACPO) while another group of 12 KKAy mice were fed with AIN‐76 feed without ACPO, for 6 weeks. Compared with the control group, the KKAy mice fed with the ACPO diet had decreased serum insulin, glycogen, fasting blood glucose, triacylglycerol, total cholesterol and low‐density lipoprotein cholesterol level, but had increased serum high‐density lipoprotein cholesterol level, linolenic acid content and total polyunsaturated fatty acid content. The results indicate that ACPO has hypoglycemic and hypolipidemic properties and may be a suitable alternative food supplement for humans.
Evaluating quantum yield and ultraviolet–visible reflectance in baby pumpkins: Implications for oviposition behavior of pumpkin fruit flies
Authors: Kim Seohyun , Kwon Ohseok , Park Ikju 
Published: November 2023

AbstractFrugivorous insects utilize both olfactory and visual cues to locate their host plants. Although volatiles have been extensively studied for detecting infested fruits, the role of visual cues in oviposition site selection remains mostly unknown among frugivorous insects. To investigate physiological changes in a host plant, we measured the quantum yield and reflectance of three wavelengths of light (350, 450 and 520 nm) after puncturing the surfaces of commercially grown pumpkins using insect pins during two different seasons outdoors. Quantum yields did not show significant differences between undamaged and simulated oviposition sites. Two wavelengths within the visual spectrum were similar between the two treatments. However, photon counts at 350 nm, in the ultraviolet range, were 1.76 fold higher in simulated oviposition sites than in undamaged sites, which was consistently observed across three field seasons. Considering that frugivorous insects and other phytophagous insects recognize the 350 nm wavelength for host identification and oviposition, we conclude that the disparities in ultraviolet reflectance can serve as a baseline for assessing the actual oviposition response of fruit flies. This finding contributes to the role of visual cues in the oviposition behavior of frugivorous insects and the development of a nondestructive detection method for pumpkin fruit flies.
Changes in root metabolites and soil microbial community structures in rhizospheres of sugarcanes under different propagation methods
Authors: Lin Xinru , Yang Da , Zhu Yu , Qin Yonglin , Liang Tian , Yang Shangdong , Tan Hongwei 
Published: November 2023

AbstractRoot metabolites and soil microbial community structure in the rhizosphere play critical roles in crop growth. Here, we assessed the efficiency of conventional and tissue culture propagation methods in modulating the soil health and microbiota in the rhizosphere of sugarcane (Saccharum officinarum L.) plants. The seeding canes were obtained using newly planted and two‐year ratooned canes propagated by conventional (CSN and CSR) or tissue culture (TCN and TCR) methods. Changes in soil fertility, root metabolites and soil microbial community structure in the rhizosphere of sugarcane plants obtained using these canes were assessed. The activities of soil β‐glucosidase and aminopeptidase, soil microbial biomass nitrogen, and abundances of soil beneficial microbes, both at phyla and genera levels, were significantly higher in the rhizosphere of sugarcane plants in TCN and TCR treatments than those in that of plants in CSN and CSR treatments. Furthermore, flavonoid and flavonol biosynthesis and alanine, aspartate and glutamate metabolism were significantly upregulated in the roots of TCR and TCN plants compared with those in the roots of CSN and CSR plants. These results suggest that the tissue culture propagation method is a sustainable method for sugarcane cultivation to improve soil fertility and health in sugarcane rhizosphere.
Comparative genomics of a vertically transmitted thiotrophic bacterial ectosymbiont and its close free‐living relative
Authors: Espada‐Hinojosa Salvador , Karthäuser Clarissa , Srivastava Abhishek , Schuster Lukas , Winter Teresa , de Oliveira André Luiz , Schulz Frederik , Horn Matthias , Sievert Stefan , Bright Monika 
Published: November 2023

AbstractThiotrophic symbioses between sulphur‐oxidizing bacteria and various unicellular and metazoan eukaryotes are widespread in reducing marine environments. The giant colonial ciliate Zoothamnium niveum, however, is the only host of thioautotrophic symbionts that has been cultivated along with its symbiont, the vertically transmitted ectosymbiont Candidatus Thiobius zoothamnicola (short Thiobius). Because theoretical predictions posit a smaller genome in vertically transmitted endosymbionts compared to free‐living relatives, we investigated whether this is true also for an ectosymbiont. We used metagenomics to recover the high‐quality draft genome of this bacterial symbiont. For comparison we have also sequenced a closely related free‐living cultured but not formally described strain Milos ODIII6 (short ODIII6). We then performed comparative genomics to assess the functional capabilities at gene, metabolic pathway and trait level. 16S rRNA gene trees and average amino acid identity confirmed the close phylogenetic relationship of both bacteria. Indeed, Thiobius has about a third smaller genome than its free‐living relative ODIII6, with reduced metabolic capabilities and fewer functional traits. The functional capabilities of Thiobius were a subset of those of the more versatile ODIII6, which possessed additional genes for oxygen, sulphur and hydrogen utilization and for the acquisition of phosphorus illustrating features that may be adaptive for the unstable environmental conditions at hydrothermal vents. In contrast, Thiobius possesses genes potentially enabling it to utilize lactate and acetate heterotrophically, compounds that may be provided as byproducts by the host. The present study illustrates the effect of strict host‐dependence of a bacterial ectosymbiont on genome evolution and host adaptation.
Plant‐derived environmental DNA complements diversity estimates from traditional arthropod monitoring methods but outperforms them detecting plant–arthropod interactions
Authors: Weber Sven , Stothut Manuel , Mahla Lisa , Kripp Alanah , Hirschler Lena , Lenz Nina , Junker Anneke , Künzel Sven , Krehenwinkel Henrik 
Published: November 2023

AbstractOur limited knowledge about the ecological drivers of global arthropod decline highlights the urgent need for more effective biodiversity monitoring approaches. Monitoring of arthropods is commonly performed using passive trapping devices, which reliably recover diverse communities, but provide little ecological information on the sampled taxa. Especially the manifold interactions of arthropods with plants are barely understood. A promising strategy to overcome this shortfall is environmental DNA (eDNA) metabarcoding from plant material on which arthropods leave DNA traces through direct or indirect interactions. However, the accuracy of this approach has not been sufficiently tested. In four experiments, we exhaustively test the comparative performance of plant‐derived eDNA from surface washes of plants and homogenized plant material against traditional monitoring approaches. We show that the recovered communities of plant‐derived eDNA and traditional approaches only partly overlap, with eDNA recovering various additional taxa. This suggests eDNA as a useful complementary tool to traditional monitoring. Despite the differences in recovered taxa, estimates of community α‐ and β‐diversity between both approaches are well correlated, highlighting the utility of eDNA as a broad scale tool for community monitoring. Last, eDNA outperforms traditional approaches in the recovery of plant‐specific arthropod communities. Unlike traditional monitoring, eDNA revealed fine‐scale community differentiation between individual plants and even within plant compartments. Especially specialized herbivores are better recovered with eDNA. Our results highlight the value of plant‐derived eDNA analysis for large‐scale biodiversity assessments that include information about community‐level interactions.
A rare cause of AA amyloidosis: Glomus tumor and treatment with anakinra—Case report and literature review
Authors: Tastekin Fatih , Kerim Duygu , Sen Sait , Kabasakal Yasemin 
Published: November 2023

AbstractSystemic AA amyloidosis is associated with poorly controlled chronic inflammatory disorders. Chronic infections and inflammatory arthritis are the most common causes; however, they can also rarely occur as a complication of neoplastic disorders. The development of AA amyloidosis secondary to paraganglioma, which is a rare type of tumor, has rarely been reported in the literature. In this case, an 85‐year‐old female patient with a glomus tumor in the neck, who has been followed up over 50 years, applied with complaints of loss of appetite, nausea, and diarrhea for 5–6 months. While evaluating the patient, who had high levels of acute phase reactants, amyloidosis was diagnosed by salivary gland biopsy. No other cause was found to explain amyloidosis. The patient, who could not tolerate treatment with colchicine and azathioprine, is successfully treated with the interleukin‐1 inhibitor anakinra. A rare relationship, systemic AA amyloidosis, which is thought to have developed as a result of long‐standing jugular paraganglioma, is presented in this article. In addition, publications showing an association between paragangliomas and amyloidosis were reviewed.
Highlighting the limitations of static microplate biofilm assays for industrial biocide effectiveness compared to dynamic flow conditions
Authors: Klopper Kyle B. , Bester Elanna , van Schalkwyk Martha , Wolfaardt Gideon M. 
Published: November 2023

AbstractThe minimal inhibitory concentration of an antimicrobial required to inhibit the growth of planktonic populations (minimum inhibitory concentration [MIC]) remains the ‘gold standard’ even though biofilms are acknowledged to be recalcitrant to concentrations that greatly exceed the MIC. As a result, most studies focus on biofilm tolerance to high antimicrobial concentrations, whereas the effect of environmentally relevant sub‐MIC on biofilms is neglected. The effect of the MIC and sub‐MIC of an isothiazolinone biocide on a microbial community isolated from an industrial cooling system was assessed under static and flow conditions. The differential response of planktonic and sessile populations to these biocide concentrations was discerned by modifying the broth microdilution assay. However, the end‐point analysis of biofilms cultivated in static microplates obscured the effect of sub‐MIC and MIC on biofilms. A transition from batch to the continuous flow system revealed a more nuanced response of biofilms to these biocide concentrations, where biofilm‐derived planktonic cell production was maintained despite an increase in the frequency and extent of biofilm sloughing. A holistic, ‘best of both worlds’ approach that combines the use of static and continuous flow systems is useful to investigate the potential for the development of persistent biofilms under conditions where exposure to sub‐MIC and MIC may occur.
The oncogenic role of LGR6 overexpression induced by aberrant Wnt/β‐catenin signaling in lung cancer
Authors: Sunaga Noriaki , Kaira Kyoichi , Shimizu Kimihiro , Tanaka Ichidai , Miura Yosuke , Nakazawa Seshiru , Ohtaki Yoichi , Kawabata‐Iwakawa Reika , Sato Mitsuo , Girard Luc , Minna John D. , Hisada Takeshi 
Published: November 2023

AbstractBackgroundMolecular abnormalities in the Wnt/β‐catenin pathway confer malignant phenotypes in lung cancer. Previously, we identified the association of leucine‐rich repeat‐containing G protein‐coupled receptor 6 (LGR6) with oncogenic Wnt signaling, and its downregulation upon β‐catenin knockdown in non‐small cell lung cancer (NSCLC) cells carrying CTNNB1 mutations. The aim of this study was to explore the mechanisms underlying this association and the accompanying phenotypes.MethodsLGR6 expression in lung cancer cell lines and surgical specimens was analyzed using quantitative RT‐PCR and immunohistochemistry. Cell growth was assessed using colony formation assay. Additionally, mRNA sequencing was performed to compare the expression profiles of cells subjected to different treatments.ResultsLGR6 was overexpressed in small cell lung cancer (SCLC) and NSCLC cell lines, including the CTNNB1‐mutated NSCLC cell lines HCC15 and A427. In both cell lines, LGR6 knockdown inhibited cell growth. LGR6 expression was upregulated in spheroids compared to adherent cultures of A427 cells, suggesting that LGR6 participates in the acquisition of cancer stem cell properties. Immunohistochemical analysis of lung cancer specimens revealed that the LGR6 protein was predominantly overexpressed in SCLCs, large cell neuroendocrine carcinomas, and lung adenocarcinomas, wherein LGR6 overexpression was associated with vascular invasion, the wild‐type EGFR genotype, and an unfavorable prognosis. Integrated mRNA sequencing analysis of HCC15 and A427 cells with or without LGR6 knockdown revealed LGR6‐related pathways and genes associated with cancer development and stemness properties.ConclusionsOur findings highlight the oncogenic roles of LGR6 overexpression induced by aberrant Wnt/β‐catenin signaling in lung cancer.
Kristen rat sarcoma virus (KRAS) G12F‐positive non‐small cell lung cancer mimicking KRAS G12C positivity: A case report
Authors: Oi Issei , Ito Takanori , Saito Zentaro , Imakita Takuma , Kanai Osamu , Fujita Kohei , Tachibana Hiromasa , Mio Tadashi 
Published: November 2023

AbstractSearching for driver gene alteration is a prerequisite for chemotherapy of non‐small cell lung cancer. Due to its high sensitivity and concordance rate, the Amoy Dx Pan Lung Cancer PCR panel has been approved and is widely used in Japan. In this report, we describe a case in which a positive result for Kristen rat sarcoma virus (KRAS) exon2 p.G12F, a rare KRAS mutation, may have led to a false‐positive result for KRAS exon2 p.G12C on AMOY. Genetic analysis in this case was performed by LC‐SCRUM‐Asia.
Sphingolipid classes and the interrelationship with pediatric asthma and asthma risk factors
Authors: Chen Yulu , Checa Antonio , Zhang Pei , Huang Mengna , Kelly Rachel S. , Kim Min , Chen Yih‐Chieh S. , Lee‐Sarwar Kathleen A. , Prince Nicole , Mendez Kevin M. , Begum Sofina , Kachroo Priyadarshini , Chu Su H. , Stokholm Jakob , Bønnelykke Klaus , Litonjua Augusto A. , Bisgaard Hans , Weiss Scott T. , Chawes Bo L. , Wheelock Craig E. , Lasky‐Su Jessica A. 
Published: November 2023

AbstractBackgroundWhile dysregulated sphingolipid metabolism has been associated with risk of childhood asthma, the specific sphingolipid classes and/or mechanisms driving this relationship remain unclear. We aimed to understand the multifaceted role between sphingolipids and other established asthma risk factors that complicate this relationship.MethodsWe performed targeted LC–MS/MS‐based quantification of 77 sphingolipids in plasma from 997 children aged 6 years from two independent cohorts (VDAART and COPSAC2010). We examined associations of circulatory sphingolipids with childhood asthma, lung function, and three asthma risk factors: functional SNPs in ORMDL3, low vitamin D levels, and reduced gut microbial maturity. Given racial differences between these cohorts, association analyses were performed separately and then meta‐analyzed together.ResultsWe observed elevations in circulatory sphingolipids with asthma phenotypes and risk factors; however, there were differential associations of sphingolipid classes with clinical outcomes and/or risk factors. While elevations from metabolites involved in ceramide recycling and catabolic pathways were associated with asthma and worse lung function [meta p‐value range: 1.863E‐04 to 2.24E‐3], increased ceramide levels were associated with asthma risk factors [meta p‐value range: 7.75E‐5 to .013], but not asthma. Further investigation identified that some ceramides acted as mediators while some interacted with risk factors in the associations with asthma outcomes.ConclusionThis study demonstrates the differential role that sphingolipid subclasses may play in asthma and its risk factors. While overall elevations in sphingolipids appeared to be deleterious overall; elevations in ceramides were uniquely associated with increases in asthma risk factors only; while elevations in asthma phenotypes were associated with recycling sphingolipids. Modification of asthma risk factors may play an important role in regulating sphingolipid homeostasis via ceramides to affect asthma. Further function work may validate the observed associations.
Swedish registry study showed that 50% of paediatric operations were performed in university hospitals and mortality was low
Authors: Pelkonen Ruut‐Maria , Frykholm Peter , Enlund Gunnar , Lilja Helene Engstrand 
Published: November 2023

AbstractAimTo investigate the distribution of paediatric surgery in various hospitals and to study postoperative risk factors of mortality.MethodsRetrospective registry‐based cohort study of children aged 0–14 years undergoing surgery from 2017 to 2021. Data were extracted from the Swedish Perioperative Registry. A mixed logistic regression was applied for the all‐risk mortality analysis.ResultsA total of 126 539 cases were identified, 50% in university, 36% in county and 14% in district hospitals. The dominating operations were appendectomy in 6667, orchidopexy in 5287, inguinal hernia repair in 4200 and gastrostomy in 1152 children. Among children under 1 year of age or American Society of Anesthesiologists Physical Status classification (ASA‐PS‐Class) 3–5, the majority underwent surgery in university hospitals. The 30‐day mortality in university hospitals was 0.5% and in county hospitals 0%. The proportion of emergency surgery was similar in university and county hospitals. Independent risk factors of mortality were being under 1 year of age, ASA‐PS‐class 4–5, emergency surgery and surgery at university hospitals.ConclusionHalf of all operations in children were performed in university hospitals, with low postoperative mortality despite effective centralisation of high risk patients <1 year of age or ASA‐PS‐Class 3–5.