bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced gene expression of potato apoplastic invertase inhibitor gene on CRISPR/Cas9 targeting and analyzing its transformation efficiency parameters
Authors: Dangol Sarbesh Das , Çalışkan Mehmet Emin , Bakhsh Allah 
Published: January 2024

Abstract

Background
Pathogen infections that affect potato yield cause severe economic losses every year. Several studies point the role of apoplastic (cell wall) invertase (CWIN) enzyme in plant defense mechanisms, and that apoplastic invertase inhibitor (INVINH1) post-translationally regulates CWIN. Nevertheless, the role of INVINH1 needs to be elucidated for several effects in plant transformation parameters and its gene expression which we sought to explore using CRISPR/Cas9 technology.
Methods and Results
In this study, we sequenced the first exon of INVINH1 gene in cv. Desiree and Solanum chacoense M6. We identified in the first exon two alleles for StINVINH1 gene in cv. Desiree and one allele for ScINVINH1 gene in S. chacoense M6. We designed two single-guided RNAs (sgRNAs) to target INVINH1 gene from diploid S. chacoense M6 and tetraploid S. tuberosum cv. Desiree using CRISPR/Cas9 based technology. In our earlier study, we have already optimized transformation protocol for M6 and cv. Desiree using Agrobacterium strains, based on which Agrobacterium strain AGL1 was chosen for CRISPR/Cas9 experiment. Our experimentation showed that heat stress at 37°C could increase the mutagenesis capability, and CRISPR/Cas9 targeting affected plant transformation parameters. It was found from the knockout experiment that the indels were present in the calli, and the candidate regenerated plants showed reduced gene expression level conducted via RT-qPCR.
Conclusion
Our study demonstrated that INVINH1 targeting affected the calli induction and regeneration rates, was effective under heat stress, and reduced its gene expression level. More studies are required to comprehend the function of INVINH1 enzyme in potato stress response and defense mechanism.
Discovery of a polyvinyl alcohol-degrading strain of the ascomycete Fusarium oxysporum and optimizing of its degradation performance of PVA
Authors: Zhang Xin , Song Juyi , Liu Chang , Chen Hui 
Published: January 2024

Abstract
Massive accumulation of plastics into environment has caused enormous pressure on the ecosystem. Efficient and environmentally friendly plastics degradation technologies have evolved into a global ecological challenge. Microbial degradation, as an eco-friendly plastic treatment technology, is confronted with a problem of low efficiency in its current application. Hence, it is crucial to discovery plastic biodegradable microorganisms and find the optimal conditions for their action. The aim of our study is to isolate plastic-biodegrading fungi and explore optimum conditions for their action. A strain isolate of Fusarium oxysporum was obtained from a degraded plastic handle through screening, separation, and purification and designated PDBF01 (CGMCC No.40272). In a screening assay of plastic polymers, PDBF01 only exhibited the degradability to polyvinyl alcohol (PVA), with no activity toward polyvinyl chloride, polypropylene, or polylactic acid. PVA degradation efficiency of PDBF01 was significantly affected by inoculum concentration, temperature, and degradation time. PDBF01 produced significant degradation of PVA under 28°C and 25% inoculum concentration. Moreover, the highest degradation rate reached 51.26% after 21 days. PVA degradation rate of PDBF01 was further increased to 58.83% by the addition of electrolytes (K+, Mg2+, Fe2+, and Ca2+). Our results suggested PDBF01 can be used as a potential and efficient PVA-degrading strain in practical applications.
Treatment of post-thalamic hemorrhage hydrocephalus: Ventriculoperitoneal shunt or endoscopic third ventriculostomy？
Authors: Lu Wenchao , Du Andong , Zheng Xiaomin 
Published: January 2024

Abstract

Background
This study aimed to compare the efficacy of ventriculoperitoneal shunt (VPS) and endoscopic third ventriculostomy (ETV) in the treatment of hydrocephalus after thalamic hemorrhage to provide reasonable surgical treatment.
Methods
The clinical data of 87 patients with hydrocephalus after TH whose external ventricular drainage(EVD) cannot be removed after hematoma absorption were retrospectively analyzed. The patients were divided into the VPS and ETV groups according to the different surgical methods. The operative time, length of hospital stay, complications, and reoperation rates of the two groups were compared.
Results
There was no statistically significant difference in intraoperative bleeding and length of hospital stay between the two groups, and all patients had the EVD tube successfully removed after surgery. There were 4 (9.5%) complications in the ETV group and 3 (6.7%) complications in the VPS group, with no significant differences in postoperative complications between the two groups.During the 1-year follow up,7 patients (16.7%) in the ETV group and 3 patients (6.7%) in the VPS group required reoperation. There was a significant difference in the reoperation rates between the two groups. In the subgroup analysis of TH with fourth ventricular hemorrhage, 6 patients (14.3%) required reoperation in the ETV group, and 1 patient (2.2%) required reoperation in the VPS group; the difference between the two groups was statistically significant.
Conclusions
ETV have good efficacy in treating hydrocephalus caused by TH and TH breaking into the lateral ventricle and the third ventricle. However, if hydrocephalus is caused by TH with the fourth ventricular hematoma, VPS is a better surgical method because the recurrence rate of hydrocephalus in ETV is higher than that in VPS. Therefore, the choice of surgical method should be based on the patient’s clinical manifestations and hematoma type.
Fabrication of New Hyper-Cross-linked polymer for Efficient Heavy metal Adsorption from Industrial Wastewater
Authors: Areej Isham , Raza Saqlain , Abid Amin , Ali Sajjad , Qureshi Ahmad Kaleem , Shafique Umer , Tan Bien , Roy Rana , Iqbal Rashid , Al-onazi Wedad A. 
Published: January 2024

Abstract
New Hyper cross-linked polymer (HCP) of Resorcinol (1, 3-dihydroxybenzol) “R-HCP” is synthesized using Friedel-Craft Reaction for the removal of cadmium metal ions from industrial wastewater. Real industrial wastewater samples are used to evaluate the adsorption capability of R-HCP. Fabricated R-HCP is characterized through Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction analysis (XRD), EDX (Energy dispersive x-ray spectroscopy) and BET (Brunauer–Emmett–Teller). The BET surface area of R-HCP is 221.5848m2g− 1.Through salt addition method, the point of zero charge (PZC) was also determined, and its value is 2.0 pH. The greatest adsorption value of 93% was obtained at pH 10 with 0.5 g of adsorbent, 9-minute contact time and favors exothermic reaction. Langmuir, Freundlich and Temkin isotherms were studied and results shows that Freundlich model is the best fit. Adsorption kinetics investigation shows that it follows pseudo first order kinetic model. Study of the effect of interfering ions including calcium, magnesium, sodium, and potassium demonstrate the decrease in adsorption capacity to little extent. R-HCP can be recycled and have regeneration capacity which is novel and distinguished feature of this adsorbent.
Digital technologies and urban inclusive growth: An empirical study based on 70 large and medium-sized cities in China
Authors: ji Xiaoqing , liu shuai , Sun Chunyun 
Published: January 2024

Abstract
Inclusive growth is an economic model aimed at reducing inequality. Currently, China is undergoing a period of rapid digital transformation. Will digital technologies affect inclusive growth? This paper utilizes panel data from 70 large and medium-sized cities in China spanning from 2011 to 2022 to examine the impact of digital technology on urban inclusive growth. The examination considers both the economic growth and social equity effects of digital technology, and further discusses the impact channels on urban inclusive growth, particularly focusing on innovation and entrepreneurship. The results indicate that digital technology can promote inclusive urban growth; in other words, the higher the level of digital technology in cities, the better the inclusive growth. From the perspective of impact channels, digital technology enhances the inclusive growth of cities through urban innovation and entrepreneurial activities. Additionally, this paper finds that the role of digital technology in inclusive growth is more pronounced in cities with low human capital compared to those with high human capital. The impact of digital technology on inclusive urban growth is distinctly evident in the eastern region, but less so in the central and western regions.
Assessment of Self-Perceived Competences in Medical Students
Authors: A Mateos Rodriguez Alonso , Sophia Denizon Arranz , Emilio Cervera Barba , Salvador Espinosa Ramirez , Raul Castañeda Vozmediano , Diana Monge Martin 
Published: January 2024

Abstract
Introduction: A professional development programme requires the identification of the most important opportunities for improvement through continuous self-assessment. In addition, a programme of competency self-perception must be validated through external observation. The aims of this study are to assess the degree of students' self-perception of the level of competence acquisition, and to study whether there is a relationship between the students' self-perception of their competence and that obtained in an external. Material and methods: To record their self-perception, the students completed a survey. Additionally, to compare the degree of self-reported competence with objectively measured clinical competence, the OSCE test from the end of the 5th year was also used. To be able to compare the self-perception items and the OSCE competency areas, an equivalence between domains was agreed by the assessors. Results: Information was collected from 93 students out of the 112. The total score for the self-perceived level of competence was found to improve significantly. All the items explored showed significantly different and increasing median scores between the three measurement times. Comparing these responses from the self-perception survey between each pair of time points where the students gave their opinion on their degree of preparation for the clinical skills practised, it can be seen that from time 1 to time 2, after the students had completed their simulation scenarios, all the dimensions were scored more highly. No significant association was found between the evaluation provided by the external OSCE assessors and that self-reported by the students at the time of taking the OSCE. Conclusion: Students perceive themselves to be more competent than their OSCE scores ultimately show. It appears that students' reported self-perception does not correlate with their respective scores on the objective test, and in principle does not seem to be a predictor of their true level of competence.
Early assessment of nephrotoxic injury in rats induced by exposure to different heavy metal solid wastes
Authors: Mu Haishuo , Wan Wangjun , PU Yanting , Kuang Rong , Deng Tongle 
Published: January 2024

Abstract
Numerous countries worldwide face significant challenges in managing Heavy metal solid wastes (HMSWs) and their associated health impacts. Despite the recognized harmful nature of heavy metals, there is a scarcity of data on the specific toxicity of HMSWs. This study utilized comparative toxicogenomic analysis of nephrotoxicity-related gene sets for heavy metals, including Fe, Cu, Co, Ni, and Mn, from the Comparative Toxicogenomics Database (CTD). Moreover, this study conducted animal experiments involving five groups of Sprague Dawley rats that were orally administered substances for a duration of 4 weeks. The control group received 1.1µM acetic acid, whereas the experimental groups were treated with leachates from copper sulfide, copper concentrate, iron ore, and cobalt-nickel-manganese ore. The rats in the treated groups exhibited reduced body weight gain, significantly decreased hematological parameters, increased white blood cell count, decreased superoxide dismutase (SOD) activity in kidney tissues, elevated malondialdehyde (MDA) levels, increased blood lactate dehydrogenase (LDH) activity, increased blood urea nitrogen (BUN), increased creatinine (Cr), and an elevated BUN/Cr ratio. Additionally, the glomerular filtration rate (GFR), BUN, and urine Cr were decreased in the treated groups. Further decision tree analysis revealed that blood LDH levels > 6.82U/gprot distinguished the treatment of cobalt-nickel-manganese and copper sulfide slag from the others, indicating the importance of blood LDH as an oxidative damage marker in assessing nephrotoxicity. Toxicogenomic analysis unveiled that the process of heavy metal- induced kidney damage involves oxidative stress, inflammatory responses, and apoptotic signaling. It also uncovered different programmed cell death processes including ferroptosis and apoptosis, in kidney cells. This study sheds light on the toxic effects of exposure to HMSWs and proposes that LDH, as an oxidative damage marker, could be pivotal in the early assessment of heavy metal-induced nephrotoxicity to safeguard public health.
Cost-effectiveness of a multi-faceted workplace intervention to prevent and manage musculoskeletal pain in nursing staff: a cluster-randomized controlled trial (INTEVAL_Spain)
Authors: Soler-Font Mercè , Aznar-Lou Ignacio , Almansa Josué , Peña Pilar , Silva-Peñaherrera Michael , Serra Consol , Ramada José Maria 
Published: January 2024

Abstract

Purpose
To evaluate the cost-effectiveness of a multifaceted workplace intervention to prevent and manage musculoskeletal pain (MSP) in nursing staff.
Methods
The study was a 1-year cluster-randomized controlled trial. The intervention combined participatory ergonomics, health promotion, and case management. The control group received usual care. Societal and health system perspectives were used. Costs included direct health and indirect costs. The effects were MSP and quality-adjusted life years (QALY). MSP was measured using the Standardized Nordic Questionnaire at baseline and 6- and 12-month follow-up. QALY were measured using the EuroQol-5D-3L at 6- and 12-month follow-up. Incremental costs and QALY were modelled using generalized linear models. MSP was analysed through generalized logistic models. Incremental cost-effectiveness ratios (ICERs) were calculated, and cost-effectiveness planes and acceptability curves were constructed.
Results
Total mean costs were €367 and €154 for the intervention and control group, respectively. The intervention costs were €38/person. From the societal perspective, the ICER showed that overall additional €12 (€6 from a health system perspective) were required to achieve 1-extra-percentage-point reduction of MSP. ICERs were €6 from the societal and €3 from the health system perspectives for neck, shoulders and upper back pain; €10 and €4 for low back; €33 and €14 for hands; €7 and €3for legs; €21 and €9 for the knees; and €7 and €3 for feet. The ICER was dominated by usual care in the elbows and QALY.
Conclusion
Although the efficiency of this intervention cannot be fully established, these findings are encouraging to reduce MSP in the workplace.
Study registration:
ISRCTN15780649, retrospectively registered.
Eliminating sensing blind spots of field-enhanced Rydberg atomic antenna via an asymmetric parallel-plate resonator
Authors: Wu Bo , zhou Yanli , Ding Zhenke , Mao Ruiqi , Qian Sixian , WAN Zhiqian , Liu Yi , Fu Yunqi 
Published: January 2024

Abstract

Due to its large electric dipole moment, the Rydberg atom exhibits a strong response to weak electric fields, hence it is regarded as a highly promising atomic antenna. However, to enhance the reception sensitivity, split-ring resonators are needed normally, which will brings sensing blind spots. Thus it is not conducive to the application of full-coverage space communication. Here we propose that an atomic antenna with an asymmetric parallel-plate resonator, can not only enhance the received signal, but also eliminate sensing blind spots (pattern roundness can reach 7.8 dB while the split-ring resonator can be up to 39 dB). We analyze the influence of structural parameters on the field enhancement factor and directionality, and further discuss the limitation of the sensitivity by using thermal resistor noise theory.This work is expected to pave the way for the development of field-enhanced Rydberg atomic antennas that communicate without a blind spot.
Influence of financial toxicity on quality of life in lung cancer patients undergoing immunotherapy: the mediating effect of self-perceived burden
Authors: Zhang Zhaoli , Xu Zhen , Yang Shikun , Huang Jingui , Huang Fengmei , Shi Yumei 
Published: January 2024

Abstract
Background
Although immunotherapy has the potential to prolong the median survival time and overall survival of patients with lung cancer, it is also a driver of immune related adverse events, which may directly affect the therapeutic effect and reduce quality of life (QOL). Attention to measure QOL in lung cancer patients undergoing immunotherapy is under-explored because of the therapy’s relative novelty. And a great deal of empirical researches has indicated the important influence of financial toxicity (FT) and self-perceived burden (SPB) on QOL. However, yet no research has ever clarified the relationship among FT, SPB, and QOL simultaneously. Hence, the purpose of this study is to understand the level of QOL of lung cancer patients undergoing immunotherapy, and attempt to clarify the potential mediating roles of SPB on the relationship between FT and QOL.
Methods
A convenience sample of 342 lung cancer patients undergoing immunotherapy was recruited from a cancer hospital from October 2022 to April 2023 in this cross-sectional study. Participants were requested to complete the structured questionnaires: sociodemographic and clinical questionnaire, Functional Assessment of Cancer Therapy–Lung (FACT-L), Self -Perceived Burden Scale (SPBS) and COmprehensive Score for financial Toxicity (COST). Data were subjected to Pearson correlation analysis and bootstrapping analysis in the structural equation modelling.
Results
The total score of FACT-L was (79.90 ± 15.84) points in 322 lung cancer patients administrated with immunotherapy. FT (β = 0.37, P < 0.01) and SPB (β = -0.27, P < 0.01) had a direct effect on QOL. In addition, SPB partly mediated the association between FT and QOL, and the standardized indirect effect was 0.19, accounting for 33.9% of the total effect.
Conclusions
The present study revealed that lung cancer patients undergoing immunotherapy experienced a lower QOL. And higher financial burden resulted in greater self-perceived burden, thus associated with inferior QOL. It is imperative for healthcare providers to routinely assessed QOL, financial toxicity or risk and self- perceived burden for lung cancer patients undergoing immunotherapy, and assist those patients in understanding the potential financial risk of each choice and help them take more active roles in their routine clinical care.
Meningioma Characteristics Influencing Overall Survival by Race and Ethnicity
Authors: Dincer Alper , Tabor Joanna , Pappajohn Alexandros , Lei Haoyi , Kim Miri , Moliterno Jennifer 
Published: January 2024

Abstract
Background There are known racial and ethnic disparities affecting overall survival (OS) in meningiomas. This has largely been presumed to be due to differences in socioeconomic status (SES). However, there are conflicting studies that demonstrate better survival for Hispanic compared to Black populations, two groups with similar SES. Thus, we hypothesize additional underlying factors, including tumor biology, may differ amongst race and influence OS of patients with meningioma.
Methods We queried the NCDB for patients with pathology-confirmed meningiomas from 2012-2020. Race and ethnicity were self-reported and grouped into White, Hispanic White, Black, Hispanic Black, and Asian categories. Logistic regression analyses were performed to determine tumor and patient characteristics associated with race and Cox hazards model was performed on these characteristics to determine influence on OS.
Results We included 85,244 patients in this study. Black patients have worse OS compared to White patients. Black patients were also more likely to harbor higher grade tumors and undergo subtotal resection compared to White patients. Hispanic White patients had improved overall survival (P<0.0001) with less higher-grade tumors (P=0.0051) compared to White patients. There was no difference in overall survival for Hispanic Black patients (P=0.49) despite being more likely to have higher grade tumors (P=0.0164).
Conclusions Differences in meningioma biology may contribute to the differences in OS seen in racial groups beyond SES. Black patients are a particularly vulnerable group as they tend to harbor higher-grade tumors and undergo less aggressive surgical resections. Further studies are necessary to determine possible differences in underlying biology.
Spondias tuberosa Arr. Câm.: a natural population in Sergipe - contributions to the understanding of genetic variability and conservation of the species
Authors: Santana Natali Aparecida , Nunes Valdinete Vieira , Silva Maria Suzana Oliveira , Silva-Mann Renata 
Published: January 2024

Abstract
The umbuzeiro, a fruit-bearing tree endemic to the Caatinga biome, stands out for its socioeconomic and environmental relevance. Although natural to Sergipe, there is a lack of information about its populations in the state. This study aimed to characterize a natural population of umbu trees in Sergipe, covering individual characteristics to the attributes of their fruits, endocarps, and seedlings. Of the 551 fruits collected from these matrices, the majority had predominantly yellow (62%) with orange (23%) peels. The endocarps, averaging 1.83 cm in length by 1.17 cm in width, significantly influenced the fresh mass of the seedlings, with larger area endocarps associated with greater mass. Endocarps stored for different periods showed no variation in germination rate and speed. These findings not only enrich the understanding of this population but also provide crucial insights for conservation and genetic improvement programs of the species in Sergipe.
Anais CIONN - Congresso Internacional de Odontologia Norte e Nordeste, Edição 2023
Authors: Gadelha Leonardo Sarmento Meira , Lima Larissa Chaves Morais de , Lima Cacilda Chaves Morais de , Palmeira Pettely Thaíse de Souza Santos , Santos Marconi Eduardo Sousa Maciel 
Published: January 2024

Editorial
Caro(a) leitor(a),
O II CIONN, Congresso Internacional de Odontologia Norte e Nordeste, realizado no Centro de Convenções de Maceió nos dias 14 a 16 de Setembro de 2023 é um projeto anual de inovação e atualização entre acadêmicos e profissionais da área da Odontologia. Nossa meta é compartilhar conhecimentos frente a uma Odontologia moderna e digital visando transformações de elevado impacto científico e social.
A comissão organizadora do CIONN reuniu os maiores nomes nacionais e internacionais para uma grade cientifica inovadora das diversas áreas do conhecimento odontológico, respeitando suas particularidades. Assim, em um espaço de exposição, reuniram-se colegas da área e empreendedores para ensinar, por meio de tecnologias de última geração.
Entre os painéis, foram submetidos mais de 400 trabalhos, dos quais 246 foram selecionados, os quais temos o prazer de divulgar no presente volume de ANAIS.
Por fim, acreditamos na internacionalização, aliada aos avanços clínicos, para o desenvolvimento científico da Odontologia.
Parabéns a todos os que vêm colhendo os frutos do CIONN!
CIONN - Congresso Internacional de Odontologia Norte e Nordeste, Edição 2023