bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pancreatic fate of a 125I‐labelled mouse monoclonal antibody directed against pancreatic B‐cell surface ganglioside(s) in control and diabetic rats
Authors: Ladriĕre Laurence , Malaisse‐Lagae Francine , Alejandro Rodolfo , Malaisse Willy J. 
Published: August 2002

AbstractThe possible use of a mouse monoclonal antibody directed against rat pancreatic B‐cell surface ganglioside(s) and labelled with radioactive iodine for selective imaging of the endocrine pancreas by a non‐invasive procedure was investigated by following its pancreatic fate in experiments conducted either in vitro by incubation of rat isolated pancreatic islets, acinar tissue and pancreatic pieces or in vivo after intravenous injection of the 125I‐labelled antibodies ([125I]γ‐G). Although the binding of [125I]γ‐G per µg protein was about one order of magnitude higher in isolated islets than in acinar tissue, no significant difference was detected when comparing pancreatic pieces or isolated islets from control animals and rats rendered diabetic by one or two prior administrations of streptozotocin (STZ rats). Likewise, except in one set of experiments, no significant difference was found between control animals and STZ rats, when measuring the radioactive content of the pancreatic gland, relative to that of plasma, 1–4 days after the intravenous injection of [125I]γ‐G. These findings indicate that under the present experimental conditions, the mouse monoclonal antibody labelled with radioactive iodine does not appear to be a promising tool for selective imaging of the endocrine pancreas, e.g. by single photon emission computerized tomography. Copyright © 2001 John Wiley & Sons, Ltd.
Functional characterization of the 5′ flanking region of human ubiquitin fusion degradation 1 like gene (UFD1L)
Authors: Amati Francesca , Conti Emanuela , Botta Annalisa , Amicucci Paola , Dallapiccola Bruno , Novelli Giuseppe 
Published: August 2002

AbstractUFD1L (Ubiquitin Fusion Degradation 1 Like) gene encodes for a component of a multi‐complex involved in the degradation of ubiquitin fusion proteins. The gene maps on chromosome 22q11, in a region commonly deleted in severe congenital disorders such as DiGeorge (DGS) and velo‐cardio‐facial (VCFS) syndromes. UFD1L is a single copy gene ubiquitously expressed in high levels in the pharyngeal pouches and fourth branchial arch artery during development. To understand the regulation of UFD1L expression we performed a functional analysis of its 5′ regulatory region. 5′‐RACE and primer extension analyses revealed the presence of different transcription start sites in adult and fetal tissues. UFD1L 5′ flanking region contains a TATA‐box motif and is also very GC‐rich with a CpG island encompassing exon 1. Transcriptional activity of this region was examined by transfection experiments of promoter‐GFP reporter gene constructs in a human epithelial cell line. These experiments revealed the importance of the region between −17 and −463 nt which contains the TATA‐box. EMSA assay resulted in the detection of five functional consensus sequences respectively for the transcription complex TFIID and for the transcription factors AP‐1 (one site), AP‐2 (one) and Sp1 (two). Copyright © 2002 John Wiley & Sons, Ltd.
Successful long‐term therapy following saphenous vein‐covered stent deployment for atherosclerotic coronary aneurysm
Authors: Kereiakes Dean J. , Broderick Thomas M. , Howard Wendy L. , Anderson Linda C. , Weber Mark , Mitts Donald L. 
Published: August 2002

AbstractWe describe a case of atherosclerotic aneurysm involving the left main coronary artery in a patient with prior coronary artery bypass surgery. A saphenous vein‐covered stent was used to seal the aneurysm in conjunction with conventional stenting of an associated native vessel coronary stenosis. Focal late restenosis involving the stent graft was successfully treated with repeat angioplasty and brachytherapy. Autologous vein‐covered stent deployment should be considered in the treatment of symptomatic or progressively enlarging coronary aneurysms. Cathet Cardiovasc Intervent 2002;55:100–104. © 2002 Wiley‐Liss, Inc.
Influence of coronary collateral flow on restenosis following primary angioplasty for acute myocardial infarction
Authors: Lee Cheol Whan , Hong Myeong‐Ki , Choi Si‐Wan , Kim June Hong , Kim Jae‐Joong , Park Seong‐Wook , Park Seung‐Jung 
Published: August 2002

AbstractWe investigated the influence of collateral flow on restenosis in 156 consecutive acute myocardial infarction (AMI) patients treated with primary angioplasty within 12 hr of symptom onset. Collateral flow was quantitatively assessed using the pressure‐derived fractional collateral flow (PDCF) index. Follow‐up angiography was performed at 6 months. The patients were classified into two groups according to the PDCF index: group I (PDCF index > 24%; n = 55) with good collaterals and group II (PDCF index ≤ 24%; n = 101) with poor collaterals. Baseline characteristics were similar between the two groups, with the exception of peak levels of creatine kinase, angiographic collaterals, and TIMI flow 3 after intervention. The binary restenosis rate was 31.8% in group I and 32.9% in group II (P = NS). Use of the stents was the only independent predictor of binary restenosis. In conclusions, well‐developed collaterals measured by PDCF may not predict restenosis following primary angioplasty for AMI. Cathet Cardiovasc Intervent 2002;55:477–481. © 2002 Wiley‐Liss, Inc.
Stenting vs. balloon angioplasty with provisional stenting for the treatment of vessels with small reference diameter
Authors: Lemos Pedro A. , Martinez Eulogio E. , Quintella Edgard , Harrell Lari C. , Ramires Jose A.F. , Ribeiro Expedito E. , da Gama Marcus N. , Horta Pedro E. , Kajita Luiz J. , Esteves Antonio , Perin Marco A. , Soares Paulo R. , Zalc Silvio , Palacios Igor F. 
Published: August 2002

AbstractA consecutive series of interventions in vessels with reference diameter ≤ 2.75 mm was retrospectively analyzed according to preprocedure strategy: balloon angioplasty with provisional stenting (PTCA group, 73 patients) and primary stenting (PS group, 122 patients). In the PS group, there were more patients with single‐vessel disease (54.1% vs. 37.0%; P = 0.021), less patients with three‐vessel disease (9.0% vs. 24.7%; P = 0.003), more LAD interventions (54.9% vs. 31.5; P = 0.002), and less left circumflex interventions (22.1% vs. 45.2%; P < 0.001). Reference diameter was larger in the PS group (2.28 ± 0.35 mm vs. 2.11 ± 0.36 mm; P = 0.001). Provisional stenting was performed in 39.7% of PTCA group. At long‐term outcome, the incidence of composite major events was similar between the PTCA and the PS groups (20.5% vs. 17.2%, respectively; P = NS). Treatment of small vessels with balloon dilatation and provisional stenting or with primary stenting yielded similar late outcomes. Operators' choice of treatment strategy was based on particular angiographic characteristics. Cathet Cardiovasc Intervent 2002;55:309–314. © 2002 Wiley‐Liss, Inc.
Successful primary angioplasty in Hughes syndrome
Authors: Osula Serge , Ramsdale David R. 
Published: August 2002

AbstractAcute myocardial infarction is a well‐recognized complication of the Hughes syndrome but its optimal treatment is uncertain. We report a case of a 42‐year‐old man with a history of previous arterial and venous thrombosis who presented with an acute myocardial infarction that was successfully treated by primary angioplasty and stenting. Cathet Cardiovasc Intervent 2002;55:482–484. © 2002 Wiley‐Liss, Inc.
A prospective single‐center registry for the use of intracoronary gamma radiation in patients with diffuse in‐stent restenosis
Authors: Nikolsky Eugenia , Rosenblatt Edward , Grenadier Ehud , Boulos Monther , Roguin Ariel , Patil Chanderashekhar , Bernstein Zvi , Huber Akiva , Ben‐Zvi Margalit , Bar‐Deroma Raquel , Markiewicz Walter , Beyar Rafael 
Published: August 2002

AbstractBased on single‐center prospective registry data, the study evaluates short‐ and long‐term results of intracoronary gamma radiation in patients with diffuse in‐stent restenosis in the reality of routine clinical practice. Percutaneous coronary intervention and subsequent catheter‐based irradiation with iridium‐192 was performed in 84 vessels (81 patients) with diffuse in‐stent restenosis. Repeat coronary angiography was performed in 35 patients with clinical restenosis. With a mean follow‐up of 12.0 ± 0.5 months, major adverse cardiac events were observed in 29 (34.5%) patients, including 2 cases of cardiac death, 3 myocardial infarctions, 21 target lesion revascularizations, and 4 target vessel revascularizations. Five of six patients with total occlusion of the target vessel at baseline developed target lesion restenosis. Late total occlusion of the target vessel was observed in four patients. The 1‐year event‐free survival rate was 69.8%. Total occlusion of the target vessel at baseline was the single independent predictor of cardiac events at 1‐year follow‐up (P < 0.001). In patients with a target lesion in the left anterior descending artery, predictors of cardiac events also included female sex (P = 0.014), current smoking (P = 0.014), stenting during brachytherapy session (P = 0.02), and smaller reference vessel diameter at baseline (P = 0.01). The results of our registry are similar to those of randomized trials. As applied in routine clinical practice, intracoronary gamma radiation is a feasible, safe, and effective tool in the treatment of diffuse in‐stent restenosis. Late events in the entire group were predicted by total occlusion at baseline. Cathet Cardiovasc Intervent 2002;56:46–52. © 2002 Wiley‐Liss, Inc.
Three separate coronary artery ostia arising from the right coronary cusp: A case report
Authors: Lauer Joseph E. , Ritchie Michael E. 
Published: August 2002

AbstractCoronary artery anomalies are uncommon in the general population and most are asymptomatic. We report a rare congenital coronary anomaly and discuss diagnosis and management strategies as well as review the pertinent literature. Cathet Cardiovasc Intervent 2001;52:496–499. © 2001 Wiley‐Liss, Inc.
Impact of abciximab versus eptifibatide on length of hospital stay for PCI patients
Authors: Lage Maureen J. , Barber Beth L. , McCollam Patrick L. , Bala Mohan , Scherer Joel 
Published: August 2002

AbstractThe purpose of this study is to compare the profile of percutaneous coronary intervention (PCI) patients who receive abciximab versus eptifibatide, as well as to compare the effect of abciximab versus eptifibatide on hospital length of stay. Retrospective data were obtained from HCIA's Clinical Pathways Database on 5,446 coronary angioplasty patients who were administered either abciximab or eptifibatide. Estimation was conducted via a two‐stage sample selection model. In the first stage, a probit regression was employed to determine which factors were associated with a higher probability of being administered abciximab versus eptifibatide. In the second stage, a negative binomial model was used to estimate the impact of a wide range of factors (selection of GPIIb/IIIa, patient demographics, insurance provider, health conditions, admission information, and hospital characteristics) on total hospital length of stay, as well as on postprocedural length of stay. After controlling for high‐risk indications and other sources of selection bias, results indicate that receipt of abciximab was associated with a significantly shorter length of total hospital stay (0.83 fewer days; P < 0.001) than receipt of eptifibatide. Additionally, receipt of abciximab was found to be associated with a significantly shorter postprocedural hospital length of stay (0.48 fewer days; P = 0.002) compared to receipt of eptifibatide. Results of this study indicate that PCI patients who are administered abciximab versus eptifibatide have a significantly shorter length of hospital stay (both total and postprocedural). This finding is important since hospital length of stay reflects the occurrence of complications and has been found to be directly related to the resources consumed during in‐patient management of patients. Cathet Cardiovasc Intervent 2001;53:296–303. © Wiley‐Liss, Inc.
Characterization of ultrasound‐detected cerebral microemboli in patients undergoing cardiac catheterization using an in vitro middle cerebral artery model
Authors: Yang Yi , Grosset Donald G. , Yang Tao , Lees Kennedy R. 
Published: August 2002

AbstractCerebral embolization has been documented as one of the complications of diagnostic heart catheterization by transcranial Doppler (TCD). This study aimed to evaluate our hypothesis that the nature of embolic signals involved in different stages of catheter manipulation may be distinct. TCD‐detected cerebral emboli occurring at different phases of cardiac catheterization were registered and differentiated by comparing their acoustic signatures with the Doppler signals generated from clinically frequently encountered embolic materials in an in vitro middle cerebral artery model. We found that there was a significant difference in embolic signal intensity and duration between different phases of cardiac catheterization. Our data suggest that different types of emboli may be involved in different phases of the catheterization. Cathet Cardiovasc Intervent 2001;53:323–330. © 2001 Wiley‐Liss, Inc.
Combined right transradial coronary angiography and selective carotid angiography: Safety and feasibility in unselected patients
Authors: Cha Kwang Soo , Kim Moo Hyun , Kim Young Dae , Kim Jong Seong 
Published: August 2002

AbstractWe evaluated the safety and feasibility of ad hoc carotid angiography following the right transradial coronary angiography. Selective carotid angiography was performed subsequent to coronary angiography in 213 consecutive patients. A 5 Fr Simmons catheter was reformed in descending or ascending aorta, then, withdrawn and rotated to cannulate the left and right carotid artery. Both carotid angiography was performed selectively in 211 (99%) patients. In two patients with severely tortuous subclavian artery, selective cannulation of the left carotid artery failed. There was no thromboembolism or arterial dissection. After the learning phase of 50 patients, the time to reform the catheter in aorta and to cannulate the left and right carotid artery was 50 ± 77, 66 ± 68, and 58 ± 57 sec, respectively. Total procedural time was 195 ± 145 sec. In conclusion, ad hoc carotid angiography can be performed reliably and safely following the right transradial coronary angiography. It might be useful for evaluation of an isolated or associated carotid artery stenosis. Cathet Cardiovasc Intervent 2001;53:380–385. © 2001 Wiley‐Liss, Inc.
Website Review: Protein‐protein interactions on the web
Authors: Wixon Jo 
Published: August 2002

AbstractWe present a brief guide to resources on the Internet relating to Protein‐Protein Interactions. These include databases containing experimentally verified and computationally inferred physical and functional interactions. There are also tools for predicting interactions and for extracting information on interactions from the literature, and organism specific databases. Copyright © 2001 John Wiley & Sons, Ltd.
Synthetic peptide and protein domain arrays prepared by the SPOT technology
Authors: Schneider‐Mergener Jens 
Published: August 2002

AbstractThe SPOT™ technology for highly parallel synthesis of peptides on flat surfaces in array type format has evolved into a versatile toolbox for a variety of applications in proteomics such as mapping protein‐protein interactions and profiling the substrate specificity of enzymes such as kinases and proteases. Originally developed for the synthesis of short overlapping peptide sequences for mapping antibody epitopes this technology has recently been extended to the synthesis of functional protein domains. This opens up a variety of future applications such as target identification and protein expression profiling. Copyright © 2001 John Wiley & Sons, Ltd.
Asymmetric induction in the 1,3‐dipolar cycloaddition of chiral nitrile oxide derived from (2R)‐bornane‐10,2‐sultam
Authors: Jóźwik Julita , Kosior Malgorzata , Kiegiel Jaroslaw , Jurczak Janusz 
Published: August 2002

AbstractThe efficient preparation of the chiral nitrile oxide derived from N‐glyoxyloyl‐(2R)‐bornane‐10,2‐sultam is presented. The nitrile oxide was trapped in situ with substituted olefins as dipolarophiles to furnish optically active 2‐isoxazolines. Chirality 13:629–630, 2001. © 2001 Wiley‐Liss, Inc.
Influence of input rate on the stereospecific and nonstereospecific first pass metabolism and pharmacokinetics of metoprolol extended release formulations
Authors: Mistry Bipin , Leslie James L. , Eddington Natalie D. 
Published: August 2002

AbstractThe purpose of this study was to examine the influence of input rate on the stereoselective and nonstereoselective pharmacokinetics of metoprolol, α‐hydroxymetoprolol, and its acid metabolite. Extended release formulations (100 mg) of metoprolol with varying release rates (e.g., slow (S), moderate (M), and fast (F)) and an oral solution (OS, 50 mg) were administered to normal, healthy extensive metabolizers. Serial blood samples were collected over 48 h, plasma was obtained, and subsequently analyzed by a validated HPLC method with fluorescence detection. The mean Tmax of metoprolol after the S, M, F (4.43, 4.00, 3.14 h, respectively) was found to be different ( P < 0.05) as compared to the OS (2.07 h). The ratio of α‐hydroxymetoprolol/metoprolol was higher for the OS (1.26) vs. the S, M, and F (1.02, 0.96, 0.99). The S/R enantiomer ratios of the concentration for metoprolol, ACMB, and α‐hydroxmetoprolol were calculated at each time point and showed a significant difference ( P < 0.05) in the absorption phase (1–4 h) vs. terminal phase (8–16 h) for fast input (solution and fast extended release formulations). Based on these results, it would appear that input rate influences the pharmacokinetics of metoprolol, its metabolites, and their enantiomers. Chirality 14:297–304, 2002. © 2002 Wiley‐Liss, Inc.
1H, 13C, and 15N NMR stereochemical study of cis‐fused 7a(8a)‐methyl and 6‐phenyl octa(hexa)hydrocyclopenta[d][1,3]oxazines and [3,1]benzoxazines
Authors: Tähtinen Petri , Sinkkonen Jari , Klika Karel D. , Nieminen Ville , STájer Géza , Szakonyi Zsolt , FüLöp Ferenc , Pihlaja Kalevi 
Published: August 2002

AbstractFour 7a‐methyl octa(or hexa)hydrocyclopenta[d][1,3]oxazines, five 8a‐methyl octa(or hexa)hydro[3,1]benzoxazines, two 6‐phenyl hexahydro[3,1]benzoxazinones, and 8a‐methyl hexahydro[1,3]benzoxazinone, all cis‐fused, were prepared and their stereostructures studied by various one‐ and two‐dimensional 1H, 13C, and 15N NMR spectroscopic methods. In solution, the cyclopentane‐fused 2‐oxo derivatives and the 1,3‐benzoxazinone were found to attain exclusively the N‐in/O‐in conformation, whereas the 6‐phenyl 2‐oxo/thioxo derivatives were found to be present predominantly in the N‐out conformation. The C‐2 unsubstituted and the 2‐oxo/thioxo 7a/8a‐methyl derivatives were all present in solution as a rapidly interconverting equilibrium of the N‐in and N‐out conformations. The C‐2 methyl derivatives were each found to be interconvertable mixtures of epimers (at C‐2) with the N‐in conformer predominating for one epimer and the N‐out conformer predominating for the other, with both predominating conformers having the C‐2 methyl group equatorially orientated. The substituent on the nitrogen (H or Me) was found to be always predominantly equatorial with respect to the heteroring, except for the epimeric 2‐methyl derivatives with N‐out conformations where steric constraints and the generalized anomeric effect resulted in the axial orientation of the C‐2 methyl being favored. Chirality 14:187–198, 2002. © 2002 Wiley‐Liss, Inc.
Four conformers characterized in allyl nitrite
Authors: Lee Sung Guen , Breeze Liam , Bohn Robert K. , True Nancy S. 
Published: August 2002

AbstractAllyl nitrite, ONOCH2CHCH2, can exist in conformations which include syn (S) and anti (A) configurations of ONOC, anti or gauche (G) configurations of NOCC, and syn or skew (Sk) configurations of OCCC. Four of them have been observed and characterized by their microwave spectra and quantum chemical calculations to have AGSk′ (0.0 kcal/mol), AGSk (0.2(2) kcal/mol), SASk (0.2(2) kcal/mol), and SAS (0.9(2) kcal/mol) configurations about the three successive dihedral angles. All four are observed in microwave studies of the vapor at 300 and 200 K and the first three are seen in pulsed‐jet beams. The SAS form apparently relaxes to the SASk conformer during expansion in the pulsed‐jet. Quantum chemical calculations reveal that the two possible conformers with anti–anti configurations of the ONOCC fragment, AAS and AASk, are transition states, unstable configurations, or of marginal stability, consistent with structures observed in this and other previously studied alkyl nitrites. The remaining four possible conformers are predicted to have relative energies within 1 kcal/mol of the observed conformers but were not observed experimentally. Chirality 14:232–240, 2002. © 2002 Wiley‐Liss, Inc.
Melatonin receptor agents: Synthesis, resolution by HPLC on polysaccharides chiral stationary phases, absolute configuration, and pharmacology of the enantiomers of (±)‐N‐[2‐(7‐fluoro‐1,2,3,4‐ tetrahydronaphthalen‐1‐yl)ethyl]acetamide
Authors: Lipka‐Belloli Emmanuelle , Guelzim Abdelhalim , Yous Said , Lefebvre Jacques , Descamps‐Francois Carole , Capet Frederic , Vaccher Claude 
Published: August 2002

AbstractIn order to obtain milligram amounts of the enantiomers of tetrahydronaphthalenic derivative 5 to be tested for binding to the melatonin sites, preparative HPLC employed a mobile phase consisting of n‐hexane‐alcohol and a silica‐based cellulose tris‐methylbenzoate (Chiralcel OJ) using isocratic conditions and multiple repetitive injections. The preparative separation was optimized by adjusting the sample size from a scale‐up of the analytical method. The enantiomeric elution order was reversed by the change from the carbamate type phase (Chiralcel OD‐H) to the benzoate type phase (Chiralcel OJ) in analytical mode. The optical rotation and the circular dichroism spectra of the single enantiomers were determined after separation. The absolute stereochemistry of the two enantiomers of (±)‐N‐[2‐(7‐fluoro‐1,2,3,4‐tetrahydronaphthalen‐1‐yl)ethyl]acetamide 5was established by X‐ray crystallographic analysis. The purity obtained was sufficient for a first screen of their biochemical properties: the (−)‐(S) enantiomer shows more affinity for melatonin receptors MT1, MT2 and is responsible of the selectivity towards MT2. Chirality 13:199–206, 2001. © 2001 Wiley‐Liss, Inc.
Identification of the configuration of neosartorin by long‐range nuclear Overhauser effect measurements
Authors: Liptaj Tibor , Pham Tran Nghia , Proksa Bohusš , UhríN Dušan 
Published: August 2002

AbstractThe relative configuration of the two xanthene units of neosartorin, a new ergochrome biosynthesised by the soil mould Neosartorya fischeri, was determined using a 1D double‐pulsed field gradient spin‐echo NOESY experiment. It was found that both units have the same relative stereochemistry. Long‐range nonbonding interactions between the substituents of different xanthene units stabilise the nonplanar configuration of the two aromatic rings A and A′ connecting both monomer units of neosartorin. Chirality 13:545–547, 2001. © 2001 Wiley‐Liss, Inc.