bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voluntary physical exercise-induced vascular effects in spontaneously hypertensive rats
Authors: HÄGG Ulrika , ANDERSSON Irene , NAYLOR Andrew S. , GRÖNROS Julia , JONSDOTTIR Ingibjörg H. , BERGSTRÖM Göran , GAN Li-ming 
Published: November 2004

Forced training has been shown to have beneficial vascular effects in various animal exercise models. In the present study, we explored possible physiological and molecular effects of voluntary physical exercise on various vascular beds. SHR (spontaneously hypertensive rats) performed voluntary exercise for 5 weeks in a computerized wheel cage facility. Ex vivo myograph studies revealed an increased sensitivity of the ACh (acetylcholine)-mediated vasodilation in resistance arteries of the exercised animals (ED50=15.0±3.5 nmol/l) compared with the controls (ED50=37.0±8.8 nmol/l; P=0.05). The exercise/control difference was abolished after scavenging reactive oxygen radicals. In conduit arteries, ACh induced a similar vasodilatory response in both groups. The in vivo aortic wall stiffness, assessed by means of Doppler tissue echography, was significantly lower in the exercising animals than in controls. This was demonstrated by significantly increased peak systolic aortic wall velocity (P=0.03) and the velocity time integral (P=0.01) in exercising animals compared with controls. The relative gene expression of eNOS (endothelial nitric oxide synthase) was similar in both groups of animals, whereas Cu/ZnSOD (copper/zinc superoxide dismutase) gene expression was significantly increased (+111%; P=0.0007) in the exercising animal compared with controls. In conclusion, voluntary physical exercise differentially improves vascular function in various vascular beds. Increased vascular compliance and antioxidative capacity may contribute to the atheroprotective effects associated with physical exercise in conduit vessels.
SEASONAL PRODUCTIVITY OF TWO RED ALGAE IN A CENTRAL CALIFORNIA KELP FOREST1
Authors: Heine John N. 
Published: November 2004

ABSTRACTThe in situ productivity of Botryocladia pseudodichotoma (Farl.) Kyl. and Rhodymenia californica var. californica Kyl., two common understory macrophytes in Macrocystis pyrifera (L.) C. Ag. forests, was determined once per month for one year. Gross productivity for B. pseudodichotoma was highest in October (1.23 mgC · g dry wt.−1· h−1), but rates were generally greater in spring and lower in summer. Respiration was variable throughout the year, especially in proportion to gross productivity. Gross productivity for R. californica var. californica was also highest in October (4.62 mgC · g dry wt.−1· h−1), and lowest in summer. Respiration was highly variable throughout the year. Deep (11 m) populations o/B. pseudodichotoma had 50% higher productivity than shallow (3 m) populations when incubated in shallow water, and 45% higher productivity when incubated in deep water. Populations of B. pseudodichotoma growing in shade at 3 m had 77% higher productivity than populations growing in sun at the same depth when incubated in a sun exposed location at 3 m. Respiration of the shade‐adapted plants was only one‐half that of the sun‐adapted plants. In comparison with similar studies in other sub‐tidal communities, net productivity of these two understory red algae is somewhat lower. Lack of strong seasonal productivity patterns and the highly variable underwater light regime suggests that understory algae may be adapted for rapid growth during short periods of high light, regardless of the time of year.
CENTROCERAS INTERNITENS N. SP. (RHODOPHYCEAE, CERAMIACEAE) FROM THE WESTERN TROPICAL NORTH ATLANTIC OCEAN1
Authors: Gallagher Susan B. , Humm Harold J. 
Published: November 2004

ABSTRACT Centroceras internitens n. sp. (Rhodophyceae, Ceramiaceae) was found in the Florida Keys and Bermuda only as an epiphyte on Ceramium nitens (C. Agardh) J. Agardh. It is described on the basis of many collections, including an abundance of reproductive material representing all stages of the life history. Laboratory culture has not been successful. The type locality is a well‐known limestone platform on the Atlantic Ocean side of West Summerland Key, Florida Keys. In addition to living material, C. internitens was found on eight herbarium specimens of Ceramium nitens at the United States National Museum, thereby establishing records of its occurrence throughout the Caribbean Sea and dating back to 1887.
VALVE MORPHOGENESIS AND THE MICROTUBULE CENTER IN THREE SPECIES OF THE DIATOM NITZSCHIA1
Authors: Pickett‐Heaps Jeremy 
Published: November 2004

ABSTRACTThe relationship of cell organelles to valve morphogenesis was investigated in three species of Nitzschia. One, N. sigmoidea (Nitzsch) W. Sm., showed consistent ability to generate both nitzschioid and hantzschioid symmetry in daughter cells following cytokinesis; the other two maintained nitzschioid symmetry stably. From previous work with Hantzschia, a certain sequence of events could be anticipated in the cytoplasm. In two significant areas–the behavior of the Microtubule Center (MC) and its microtubule (MT) system, and the central origin of the silicalemma–not only were the results unexpected, but the three species showed fundamental differences among themselves. In N. sigmoidea, the silicalemma (and the future raphe region) arises centrally on the cleavage furrow, and after some lateral expansion, the silicalemmas and their associated organelles move in opposite directions in daughter cells, so that the raphe and the raphe canals end up along the girdle side of the cell as expected. However, the MCs never become associated with their silicalemma, remaining throughout near the girdle bands. In N. sigma (Kütz) W. Sm., the silicalemmas arise centrally and after lateral growth, move in opposite directions to generate nitzschioid symmetry. In this case, the MCs move to the vicinity of but never close to the silicalemmas, and follow them distantly during their lateral movement. In N. tryblionella Hantzsch, the new silicalemmas arise opposite one another, on one side of the daughter cells; each MC soon moves very close to its silicalemma, and remains thus through most of valve morphogenesis. Later, only one silicalemma/MC complex moves laterally, establishing the nitzschioid symmetry in both daughter cells. In all three species, as in Hantzschia, linear arrays of mitochondria aligned along MTs occupy the forming raphe canal, and microfilaments line the outer edge of the expanding silicalemma. The fibulae (the wall struts arching across the raphe canal) in Hantzschia always grow from the valve surface to the girdle surface of the forming valves. In these three Nitzschiae, this invariably happens in only one daughter cell of any pair; in the other, all the fibulae grow from the girdle surface to the valve surface. An explanation of these variations is proposed: that the morphogenetic machinery of Nitzschia and Hantzschia have a common origin, with present Nitzschiae having undergone considerable diversification at the intracellular level, causing the unstable cell symmetry exhibited by several modern species. Perhaps a taxonomic distinction between Hantzschia and Nitzschia lies in whether the morphogenetic machinery associated with valve morphogenesis moves laterally in the same or in opposite directions.
NTAGONISM BETWEEN CADMIUM AND IRON IN THE MARINE DIATOM THALASSIOSIRA WEISSFLOGII1
Authors: Harrison Gail I. , Francois M. M. Morel 
Published: November 2004

ABSTRACTCadmium inhibits iron uptake and assimilation in the coastal diatom Thalassiosira weissflogii Grun. The effect of cadmium on short term Fe uptake fits ft competitive binding model:

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where (Fe3+) and (Cd2++) tire the free ferric and cadmium ion concentrations, respectively. The apparent binding constant Kcds, is calculated to be ca. lO8.2M‐1 compared to a Kfe of lO19 M‐1. At low free ferric ion concentrations. interference of cadmium with iron transport (at pCd = 8 and pFe* < 20) results in a simultaneous decrease in growth rate and Fe accumulation to a level known 1o limit growth. Upon decreasing the free cadmium ion concentration, cells accumulate a large amount oj iron prior to resumption of normal growth. At higher free ferric ion concentrations (pFe* < 20) normal or elevated Fe quotas are absented but “luxury consumption” of iron still occurs upon reversal of toxicity. Evidence that these algae with high cellular iron quotas are effectively Fe deficient is provided by a decrease in the cytochrome f/chlorophyll a ratio and a much greater decrease in NO3‐ reductase activity than in aldolase activity or H14C03 assimilation. Under the conditions of this study, cadmium had little effect on Si accumulation. The transport of methylamine (an analog of NH+4) is unaffected by short term exposure to high free cadmium ion concentration but is greatly inhibited upon long term (97 h) exposure.
Feeding insects with a phytopathogenic fungus influences their diapause and population dynamics
Authors: Mondy Nathalie , Corio‐Costet Marie‐France 
Published: November 2004

Abstract.  1. A facultative mutualistic relationship between a herbivorous moth, Lobesia botrana, and a phytopathogenic fungus, Botrytis cinerea, both damaging the same plant, the vine, has been demonstrated recently. Laboratory and field studies were carried out to determine the influence of the presence of the fungus in food on larvae during the pre‐ and post‐diapause periods and on the global fitness of the insect.2. During the pre‐diapause period, larvae fed on fungus exhibited a higher survival rate and a faster larval development than insects reared without fungus.3. After the wintering diapause, insects reared with fungus displayed a better synchronisation of adult emergence and an increased fecundity compared with control insects.4. These results indicate that food containing fungus provides an improved diet, permitting the herbivorous insect to obtain nutrients required to optimise their life‐history traits.5. In addition, the results emphasise the importance of the nutritional quality of larval diet, improved by addition of the fungus, on diapause termination and on the demography of the insect before and after the wintering diapause.
Influence of a bimodal emergence strategy of a Dipteran host on life‐history traits of its main parasitoids
Authors: Fournet S. , Astier N. , Cortesero A. M. , Biron D. G. 
Published: November 2004

Abstract.  1. Among a great diversity of other strategies, insects have evolved polymodal emergence patterns that can increase survival in the face of annual variations in environmental conditions. Delia radicum L. (Diptera: Anthomyiidae) is a polyvoltine pest that attacks several cultivated cruciferous species. At the time of emergence, most populations show a polymodal emergence of type A (i.e. all individuals that enter diapause in the same year terminate diapause the following growing season, and exhibit a bimodal emergence curve). This results in the occurrence of two sympatric phenotypes, early and late, which differ by the timing of adult emergence in both diapausing and non‐diapausing generations.2. In Brittany, D. radicum pupae can be heavily parasitised by three parasitoids, Aleochara bilineata Gyll. (Coleoptera: Staphylinidae), Aleochara bipustulata L., and Trybliographa rapae (Hymenoptera: Eucoilidae). As in all parasitoids species, the successful development of both Aleochara species and T. rapae larvae depends mainly on the amount and quality of the food provided by the host.3. The relationship between early and late host phenotype and (i) the parasitism efficiency, (ii) emergence patterns, and (iii) host selection behaviour were investigated for the three parasitoid species.4. Depending on parasitoid species and their different parasitism and development mode, the results reveal that host phenotype can influence (i) survival and development time in T. rapae, and (ii) survival in Aleochara species. Aleochara larvae did not appear to discriminate between early and late host pupae on the basis of phenotype, but rather selected them according to their developmental stage. Furthermore, it was discovered that the same phenological strategy occurred in T. rapae and in D. radicum. However, for D. radicum the results indicate that such a strategy has a cost as the longer development time of late host pupae results in a longer period of time favourable for parasitism.
Down‐regulation of pathogenic autoantibody response in a slowly progressive Heymann nephritis kidney disease model
Authors: Barabas Arpad Z. , Cole Chad D. , Barabas Arpad D. , Lafreniere Rene 
Published: November 2004

SummaryIn the present article, we describe an antigen‐specific down‐regulation of a pathogenic autoantibody (aab)‐mediated disease process in an experimental autoimmune kidney disease in rats called slowly progressive Heymann nephritis (SPHN). This autoimmune disease is initiated and maintained by pathogenic immunoglobulin G (IgG) autoantibodies (aabs), which cause an immune‐complex (IC) glomerulonephritis associated with proteinuria. We achieved down‐regulated pathogenic aab response in SPHN rats by injections of an IC containing the native nephritogenic antigen and specific high‐titred nonpathogenic IgM aabs, in antigen excess. The injected IC increased the level of circulating nonpathogenic IgM aabs; the increased levels of specific IgM aabs in turn facilitated the removal of the injected altered nephritogenic and liberated autoantigens from the renal tubules and greatly diminished the production of pathogenic aabs and the build up of immune deposits in the glomeruli. While animals treated early had advantages over rats whose kidney disease was well established before treatment; animals treated late into the disease still manifested noticeable improvements in similar areas, i.e. with lessened proteinuria, kidney lesion reduction and a decreased pathogenic aab response. At the end of the experiment at 29 weeks, 80% of all the treated rats had insignificantly low levels of circulating IgG aabs, indicating cessation of pathogenic aab production and corresponding termination of the disease process. In contrast, most untreated rats with the kidney disease still had high levels of circulating pathogenic aabs at the end of the experiment, which maintained disease progression.
Production performance and meat quality of grazing lambs finished on red clover, lucerne or perennial ryegrass swards
Authors: Fraser M. D. , Speijers M. H. M. , Theobald V. J. , Fychan R. , Jones R. 
Published: November 2004

AbstractThe combined benefits of a high crude protein concentration, and possible protein protection and growth‐promoting properties, make forage legumes potentially attractive as a natural means of increasing liveweight gain and time to slaughter of lambs in lamb finishing systems. An experiment was conducted to compare the production performance and meat quality of grazing lambs finished on red clover (Trifolium pratense), lucerne (Medicago sativa) or perennial ryegrass (Lolium perenne) swards. Replicate (n = 2) swards of red clover, lucerne and perennial ryegrass were rotationally grazed by ten ram lambs and ten ewe lambs from weaning until selection for slaughter at UK fat class 3L. Lambs grazing the red clover sward had a significantly higher liveweight gain and required significantly fewer days to slaughter than lambs grazing the lucerne sward (305 g d−1 vs. 243 g d−1; 38 d vs. 50 d), which in turn had a higher liveweight gain and required fewer days to slaughter than lambs grazing the perennial ryegrass sward (184 g d−1; 66 d). Lambs grazing the red clover and lucerne swards had significantly higher herbage intakes than those grazing the perennial ryegrass sward (2·06, 1·72 and 1·16 kg DM d−1 respectively), but in vivo digestibility of herbage was similar. Lambs grazing the red clover and lucerne swards also had significantly higher serum urea concentrations than those grazing ryegrass (12·5, 11·1 and 6·2 mmol L−1 respectively). Killing‐out percentage was significantly higher for lambs grazing the red clover sward than for lambs grazing the perennial ryegrass sward (48% vs. 46%). There were no significant effects of finishing system on meat flavour, but meat from lambs finished on the lucerne sward was oxidatively less stable than that from lambs finished on the perennial ryegrass sward. Grazing the forage legume swards significantly increased the proportion of linoleic and linolenic acid in muscle tissue, and therefore the proportion of unsaturated to saturated fatty acids (0·19, 0·16 and 0·12 for the red clover, lucerne and perennial ryegrass swards respectively). However, the n−6/n−3 ratio was significantly lower for the muscle of lambs grazing the perennial ryegrass sward compared with those grazing the forage legume swards (1·13, 1·08 and 0·98 for the red clover, lucerne and perennial ryegrass swards respectively). The results indicate that by grazing lambs on forage legume swards it is possible to increase individual lamb performance without compromising meat quality.
Unravelling the microfacies signatures of parasequences using computer‐optimized similarity matrices
Authors: Spence Guy H. , Arnaud‐Vanneau Annie , Arnaud Hubert , Tucker Maurice E. 
Published: November 2004

AbstractThe microfacies of a Lower Cretaceous carbonate drillcore from Oman are characterized using optimizing matrices of Jaccard's similarity coefficients of community. Other than systems tract boundaries, there is no obvious evidence of individual parasequences in the core. However, diagnostic patterns in microfossil distribution identify environmental gradients recording changes in water depth. These gradients are used to define individual parasequences, parasequence sets, stacking patterns and key surfaces. The patterns suggest that deposition was controlled by successive fourth‐ to fifth‐order (high‐frequency) relative sea‐level cycles superimposed on an underlying third‐order relative sea‐level rise. Although the correlation of these depositional subunits to systematic changes in water depth and the rate of carbonate accumulation alone is not incontrovertible proof of such a sea‐level control, concurrent multiorder relative sea‐level cyclicity provides by far the most likely explanation. A microfacies deposited when the water depth was shallowing is characterized by a relay of microfossils with affinities that shallow upwards. Conversely, a microfacies that records a gradual increase in water depth has a relay of microfossils with affinities that deepen upwards. Microfacies characterized by an assemblage of microfossils with similar affinities record deposition when the benthic environmental conditions remained stable, either because of an equilibrium between shallow water carbonate deposition and rising sea level, or in deeper water where sediment composition was relatively insensitive to changes of water depth. Microfacies characterized by mixed affinity assemblages record syndepositional reworking. During periods of embedded multiorder sea‐level changes, individual parasequences within systems tracts are shown to record more complex environmental gradients than simply the repetition of successive shallowing‐up units as traditionally represented in carbonate sequence stratigraphic models. The microfacies of an individual parasequence may shallow up, or may record both deepening‐up and shallowing‐up depositional phases, as well as periods of sedimentation when benthic environments remained stable. Individual parasequence boundaries may be submarine or subaerial unconformities, or be conformable, as part of a predictable stratigraphic pattern related to the temporal position of an individual parasequence within the underlying third‐order cycle of relative sea‐level change. The hitherto ubiquitous use of assemblages to describe carbonate microfacies, coupled with the widespread use of the metre‐scale shallowing‐up template to identify parasequences, may have led to such complexities previously being overlooked.
Stability of coagulation factors in thawed, solvent/detergent‐treated plasma during storage at 4 °C for 6 days
Authors: Buchta C. , Felfernig M. , Höcker P. , Macher M. , Körmöczi G. F. , Quehenberger P. , Heinzl H. , Knöbl P. 
Published: November 2004

Background and Objectives  Transfusion of fresh‐frozen plasma is still a pillar in emergency medicine for using to prevent dilutional coagulopathy or disseminated intravascular coagulation after severe blood loss, but thawing procedures can delay its availability. On the other hand, the wastage of plasma, once thawed and not transfused within a defined time‐period, represents an inefficient handling of economic resources and is contradictory to blood donor intentions. In this study we investigated the stability of coagulation factor activities and plasma protein levels during 6 days of storage of thawed solvent/detergent (S/D)‐treated plasma at +4 °C. Our results may form the basis for reconsideration of expiry times of thawed S/D‐treated plasma.Materials and Methods  Five units of S/D‐treated plasma (Octaplas®) were thawed and warmed to 20 °C, then recooled and stored at +4 °C for 6 days. The activities of coagulation factors II, V, VII, VIII, IX, X, XI and XII, fibrinogen, antithrombin (AT), protein C, protein S and von Willebrand factor antigen (vWF:Ag) were measured on days 0, 1, 2, 3 and 6.Results  Except for protein S, the activities of all coagulation factors and inhibitors were at least 0·5 U/ml during storage at 4 °C for 6 days. The mean levels, during storage, of factors IX, X, XI and XII, vWF:Ag, fibrinogen and protein C were at least 94%, and of factors II, V and VIII, and AT at least 78%, of the levels immediately after thawing; the activity of factor VII decreased to 83% and of protein S to 43% of the baseline values.Conclusions  Thawed S/D‐treated plasma stored at +4 °C for up to 6 days still contains sufficient coagulation activities and plasma proteins to be regarded as suitable for transfusion in the established indications.
The number of ribs and vertebrae in a Piétrain cross: variation, heritability and effects on performance traits
Authors: Borchers N. , Reinsch N. , Kalm E. 
Published: November 2004

SummaryIn a three‐generation experiment with five purebred Piétrain boars and one Landrace, one Large White and 12 Landrace × Large White crossbred sows as founder animals the number of ribs and presacral vertebrae was determined. These numbers were recorded for 111 F1‐animals and for 2711 F2‐animals. The number of ribs varied from 13 to 17 and the number of presacral vertebrae ranged from 26 to 30. Means of 15.5 for the number of ribs and 28.6 for the number of vertebrae with a standard deviation of 0.6 for both were calculated. Bayesian heritability estimates were 0.51 for the rib number and 0.62 for the vertebrae number. A phenotypic correlation of 0.76 and a genetic correlation of 0.97 indicated a close relationship of both traits. In additional analyses possible direct or linked effects of rib and vertebrae number on different growth and carcass traits were investigated. Significant effects were found for several traits related to growth, fatness, muscularity and meat quality. Genes for the presacral vertebrae number or linked quantitative trait loci (QTL) accounted for 11.5% of the phenotypic variation in carcass length and for 2.4% of the phenotypic variation in ham weight.