bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving turn-around times in low-throughput distributed hematology laboratory settings with the CellaVision® DC-1 instrument
Authors: Mayes Cheri , Gwilliam Tracey , Mahe Etienne R. 
Published: November 2023

Abstract

Objectives
Digital pathology is becoming standard in the delivery of timely, high-quality clinical services, inclusive of morphological assessment in laboratory hematology. While many digital hematology systems are designed with high-throughput in mind, CellaVision® has recently developed a low-throughput instrument, the CellaVision® DC-1. The utility of the CellaVision® DC-1 was tested in a distributed laboratory system, with a focus on turn-around times (TATs).

Methods
We evaluated the TATs of a CellaVision® DC-1 workflow, with specimens originating in a small spoke-laboratory referring materials to a central hub-laboratory. Our spoke-laboratories perform on-site complete blood counts (CBC’s) and manual peripheral blood smears (PBS’s), with complex cases referred for review to the hub-laboratory. Baseline TATs were collected, followed by prospective evaluation of 21 cases analyzed using the CellaVision® DC-1, with digital review by spoke-laboratory staff in concert with remote review by hub-laboratory staff. The TATs for the same 21 cases by standard manual assessment were compared.

Results
Improvement in the distribution of TATs using the CellaVision® DC-1 was noted relative to the retrospective spoke-laboratory data (Mann–Whitney U=26, p<0.0001) and the parallel manual PBS review (Wilcoxon W=190, p<0.0001). The CellaVision® DC-1 permitted a significant reduction in case-assessment times (Wilcoxon W=105, p=0.0001). No significant diagnostic discrepancies were identified during the testing timeframe.

Conclusions
We describe a real-world assessment of the CellaVision® DC-1 analyzer in a distributed (hub-and-spoke) laboratory network, linking low-volume laboratories to high-throughput sites. Our evaluation highlights significant improvements in case TATs with a CellaVision® DC-1 assisted digital pathology workflow.
KMT2A maintains stemness of gastric cancer cells through regulating Wnt/β-catenin signaling-activated transcriptional factor KLF11
Authors: Deng Chongwen , Ye Chunhua , Liao Xiwang , Zhou Fuyin , Shi Youxiong , Zhong Hong , Huang Junbiao 
Published: November 2023

Abstract
The molecular mechanisms of epigenetic regulation in gastric cancer development are not yet well established. In this study, we demonstrated that KMT2A was highly expressed in gastric cancer and associated with poor outcomes of patients and revealed that KMT2A was significantly associated with stemness and increased nuclear β-catenin in gastric cancer. Mechanistically, KMT2A activated the translocation of β-catenin into the nucleus of gastric cancer cells, and then, β-catenin served as a coactivator of KLF11, which promoted the expression of specific gastric cancer stemness-related molecules, including SOX2 and FOXM1. Together, KMT2A is an important epigenetic regulator of gastric cancer stemness, which provides a novel insight to the potential application of targeting against KMT2A in treating gastric cancer.
Space-time evolution characteristics of loaded gas-bearing coal fractures based on industrial μCT
Authors: Zhang Hang , Zhang Tianjun , Pang Mingkun , Wang Dengke , Zeng Fanchao 
Published: November 2023

Abstract
The space-time evolution characteristics of fractures in coal seams under external loads are of great significance for the exploration and development of coalbed methane and the safe mining of coal. In this study, the in situ industrial μCT scanning experiments of gas-bearing coal under triaxial compression conditions was carried out using a loaded coal rock industrial μCT scanning system. The computed tomography (CT) scan images of different deformation stages were obtained. The temporal characteristics and spatial evolution patterns of fractures during gas-bearing coal rupture were investigated by combining image retrieval techniques and fractal theory. The results show that (1) as the axial load increases, the maximum similarity of the CT images showed a gradually decreasing trend. (2) Under the triaxial compression conditions, the number of two-dimensional cracks in a coal-containing gas at different spatial positions exhibited a changing pattern in which it slowly decreased before rapidly increasing. (3) The patterns of change in the three-dimensional fracture rate, fracture density, and fractal dimension were quite similar, showing four stages of slow decline, slow growth, sharp growth, and slow growth again. In contrast, the Euler number exhibited the exact opposite pattern of change. (4) The coal sample space showed prominent zonal failure characteristics. The bottom of the coal sample space position (Area C) near the loading indenter had the most developed cracks and serious damage, the middle of the coal sample space position (Area B) was the second, and the upper of the coal sample space position (Area A) near the fixed indenter had slow fracture development and minimal damage.
Determination of the Number of Breaks in High-Dimensional Factor Models via Cross-Validation
Authors: Zhou Ruichao , Wang Lu , Wu Jianhong 
Published: November 2023

Abstract
This paper proposes a cross-validation method to estimate the number of breaks in high-dimensional factor models. To preserve the original change structure, the parity-splitting strategy is adopted when employing the cross-validation method. The consistency of the estimator is established under some mild conditions. Simulation results show desired finite sample performance of the proposed method, especially in comparison with methods that need to predetermine the tuning parameters.
Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy
Authors: So Wing Yan , Liao Yilie , Liu Wai Nam , Rutter Guy A , Han Weiping 
Published: November 2023

AbstractLoss of pancreatic beta cells is the central feature of all forms of diabetes. Current therapies fail to halt the declined beta cell mass. Thus, strategies to preserve beta cells are imperatively needed. In this study, we identified paired box 6 (PAX6) as a critical regulator of beta cell survival. Under diabetic conditions, the human beta cell line EndoC‐βH1, db/db mouse and human islets displayed dampened insulin and incretin signalings and reduced beta cell survival, which were alleviated by PAX6 overexpression. Adeno‐associated virus (AAV)‐mediated PAX6 overexpression in beta cells of streptozotocin‐induced diabetic mice and db/db mice led to a sustained maintenance of glucose homeostasis. AAV‐PAX6 transduction in human islets reduced islet graft loss and improved glycemic control after transplantation into immunodeficient diabetic mice. Our study highlights a previously unappreciated role for PAX6 in beta cell survival and raises the possibility that ex vivo PAX6 gene transfer into islets prior to transplantation might enhance islet graft function and transplantation outcome.
Transcriptional profiling of Kiss1 neurons from arcuate and rostral periventricular hypothalamic regions in female mice
Authors: Manchishi Stephen , Prater Malwina , Colledge William Henry 
Published: November 2023

In brief
The transcriptional profiles of Kiss1 neurons from the arcuate and the rostral periventricular region of the third ventricle of the hypothalamus have been directly compared in diestrous female mice. Differentially expressed genes provide molecular signatures for these two populations of Kiss1 neurons and insights into their physiology.

Abstract
The neuropeptide kisspeptin is produced by Kiss1 neurons and is required for normal mammalian fertility. The two main populations of Kiss1 neurons are located in the arcuate (ARC) and the rostral periventricular area of the third ventricle (RP3V) of the hypothalamus. To define the molecular signature of these Kiss1 populations, transcriptomics profiling was performed using purified Kiss1 neurons from diestrous stage female mice. From a data set of 7026 genes, 332 differentially expressed transcripts were identified between the Kiss1ARC and Kiss1RP3V neurons. These data have uncovered novel transcripts and expanded the receptor expression, co-transmitter and transcription factor profiles of Kiss1 neurons. Validation by quantitative RT-PCR confirmed differential expression of Cartpt, Ddc, Gal, Gda, Npy2r, Penk, Rasp18, Rxfp3, Slc18a2, and Th in Kiss1RP3V neurons and Gpr83, Hctr2, Nhlh2, Nmn, Npr3, Nr4a2, Nr5a2, Olfm2, Tac2 and Tacr3 in Kiss1ARC neurons. Enriched pathways common to both Kiss1 populations included the NF-kB, mTor, endocannabinoid, GPCR, Wnt and oestrogen signalling while some pathways (e.g. cytomegalovirus infection, dopaminergic and serotonergic biosynthesis) were specific to Kiss1RP3V neurons. Our gene expression data set augments the existing data sets describing the transcriptional profiles of Kiss1 neuronal populations.
Estudio de las transiciones de fase inducidas por tratamiento térmico de temple y revenido en el acero 1045.
Authors: Trujillo Alejandro , Guerrero Rosalba , Bravo Jorge 
Published: November 2023

In this work we report the characterization of a sample of steel 1045 subjected to the thermal treatment of quenching in water and quenching and tempering. The sample was made available by the Bholer,Company, Peru. Taking as reference the results of this characterization, the phase transformations that take place when a sample is subjected to the above mentioned treatments, are studied. The applied techniques were optical microscopy, dispersive Mössbauer spectroscopy and X ray fluorescence . The images by optical microscopy of the sample in its as received state, as “acero-ST”, reveals the presence of ferrite and perlite. The image of the sample quenched in water identified as ‘acero-agua’ reveals the presence of martensite; and the sample in the quenching and tempering state, denominated “acero –agua R” reveals the presence of the denominated tempered martensite. In addition, the measurements of hardness were carried out obtaining 219 HB, 634 HB and 525 HB for the samples acero-ST, acero-agua and acero-agua R respectively. The Mössbauer spectrum of the “acero-ST” sample exhibits two sextets associated to ferrite and perlite. The Mössbauer spectrum for the sample “acero-agua” exhibits the presence of three magnetic sextets assigned to iron sites present in matensite, that are occupied by the first three neighbors of each carbon atom. The Mössbauer spectrum that corresponds to the “acero-agua R” shows three magnetic sextets associated to annealed martensite. In addition to iron, the X-ray fluorescence spectra of the samples under study show the presence of manganese, copper and chromium. We pretend that these obtained results will contribute to a better knowledge of the phase transformations that take place in Steel 1045 in the tempered in water and normalized states. Moreover, we look forward for better cooperation ties between the industrial and the academic sectors.
Social Drivers of Health and Pediatric Extracorporeal Membrane Oxygenation Outcomes
Authors: Alizadeh Faraz , Gauvreau Kimberlee , Mayourian Joshua , Brown Ella , Barreto Jessica A. , Blossom Jeff , Bucholz Emily , Newburger Jane W. , Kheir John , Vitali Sally , Thiagarajan Ravi R. , Moynihan Katie 
Published: November 2023

BACKGROUND
Relationships between social drivers of health (SDoH) and pediatric health outcomes are highly complex with substantial inconsistencies in studies examining SDoH and extracorporeal membrane oxygenation (ECMO) outcomes. To add to this literature with emerging novel SDoH measures, and to address calls for institutional accountability, we examined associations between SDoH and pediatric ECMO outcomes.

METHODS
This single-center retrospective cohort study included children (&lt;18 years) supported on ECMO (2012–2021). SDoH included Child Opportunity Index (COI), race, ethnicity, payer, interpreter requirement, urbanicity, and travel-time to hospital. COI is a multidimensional estimation of SDoH incorporating traditional (eg, income) and novel (eg, healthy food access) neighborhood attributes ([range 0–100] higher indicates healthier child development). Outcomes included in-hospital mortality, ECMO run duration, and length of stay (LOS).

RESULTS
540 children on ECMO (96%) had a calculable COI. In-hospital mortality was 44% with median run duration of 125 hours and ICU LOS 29 days. Overall, 334 (62%) had cardiac disease, 92 (17%) neonatal respiratory failure, 93 (17%) pediatric respiratory failure, and 21 (4%) sepsis. Median COI was 64 (interquartile range 32–81), 323 (60%) had public insurance, 174 (34%) were from underrepresented racial groups, 57 (11%) required interpreters, 270 (54%) had urban residence, and median travel-time was 89 minutes. SDoH including COI were not statistically associated with outcomes in univariate or multivariate analysis.

CONCLUSIONS
We observed no significant difference in pediatric ECMO outcomes according to SDoH. Further research is warranted to better understand drivers of inequitable health outcomes in children, and potential protective mechanisms.
„Homilie to często klęska”. Konieczność poprawy jakości homilii
Authors: Sławiński Henryk 
Published: November 2023

This article deals with the newest history of the Church’s efforts to improve the quality of homilies. The source material is the texts contained in documents from various synods in the 21st century dealing with the issue of homilies and documents from various stages of the synod on synodality, as well as the published results of research conducted by theologians in Poland on some given homilies. The conducted analyzes allows for the conclusion that the participants of the Holy Mass greatly appreciate listening to homilies and expect them, even on weekdays. A critical assessment of the quality of the homily concerns the poverty of doctrinal content, poor grounding in the celebrated mystery, and the lack of coherence of moral indications with the proclaimed message. In a short summary, some suggestions to improve the quality of homilies are proposed.
New data from a subanalysis of the TRICOLOR study: antihypertensive effectiveness of the triple single-pill combination of amlodipine/indapamide/perindopril and predictors of effectiveness and blood pressure control in young patients
Authors: Karpov Yu. A. , Logunova N. A. , Kvasnikov B. B. , Khomitskaya Yu. V. 
Published: November 2023

Aim. To describe the antihypertensive efficacy of triple fixed­dose combination of amlodipine/indapamide/perindopril and assess the predictors of efficacy in young patients (<50 years).Material and methods. The TRICOLOR study (NCT03722524) is an observational prospective study (n=1247) that demonstrated high antihypertensive effectiveness and good tolerability of the triple single­pill combination (SPC) of amlodipine/ indapamide/perindopril. This subgroup analysis was performed on 199 patients aged <50 years (16% of the total population), and the comparison group consisted of 925 patients (82,3% of the total population) aged 50 years or older.Results. In young patients, during triple therapy with SPC amlodipine/indapa­ mide/perindopril, a positive trend in blood pressure (BP) reduction was observed compared to the baseline: an average decrease in BP after 12 weeks was 32,6 (11,0)/14,8 (8,5) mm Hg (p<0,0001), comparable to patients over 50 years of age in terms of reduction in systolic blood pressure (SBP) and diastolic blood pressure (DBP) after 2, 4 and 12 weeks of follow­up. A greater number of young patients achieved a reduction in blood pressure <140/90 mm Hg after 2 weeks of therapy compared to patients 50 years of age and older (49,7% vs 38,8%, p=0,004), and blood pressure <130/80 mm Hg – after 4 and 12 weeks (51,3% vs 43,5% (p=0,041) and 74,9% vs 67,5% (p=0,038), respectively). Left ventricular hypertrophy was a significant negative predictor of SBP reduction by 12 weeks of therapy, and the presence of grade 2 hypertension (HTN) and statin use, on the contrary, were positive predictors of changes in SBP by the end of observation. More adherent younger patients were significantly more likely to have a decrease in DBP by 12 weeks of follow-up. In addition, in young patients, male sex and the presence of dyslipidemia significantly increased the chances of blood pressure control at the end of observation. Waist circumference, body mass index, and grade 2 HTN were negative predictors of achieving the target blood pressure level.Conclusion. Thus, in young patients, good antihypertensive effectiveness of amlodipine/indapamide/perindopril was observed, comparable in the degree of blood pressure reduction with the older age group of 50 years and older.
Objectives of State Human Rights Protection Activities in the Russian Federation
Authors: Chechelnitskiy I. V. 
Published: November 2023

The paper defines the author’s approach to the concept, types, characteristics of the goals of state human rights protection. In its most general form, human rights protection includes a system of actions carried out by State bodies and organizations, as well as civil society institutions and international organizations, aimed at restoring violated and creating appropriate conditions for implementation of human and citizen rights, freedoms and legitimate interests, as well as collective rights and public interests of groups of citizens, commercial and non-commercial organizations.Researchers propose to distinguish the following types of human rights protection objectives: general and particular; basic and non-basic; final, intermediate and immediate; objectively true and objectively false; real and unreal; timely and untimely; substantive and instrumental. Indeed, the available academic developments on this issue are of great interest, but largely they characterize the human rights objectives enshrined in legislation, rather than practice of human rights protection.The paper elucidates and describes in detail such necessary signs of a human rights protection objective as its humanism, moral validity, social conditionality and legal significance.The classification of the types of human rights objectives is defined; it is proposed to distinguish strategic (general) and tactical (private) objectives; law enforcement, law-making and «law-enlightening» ones; positive and natural law human rights objectives.The paper concludes that there is a need for comprehensive consideration of the goals of human rights protection in legislation and law enforcement practice. It is also indicated that a necessary characteristic of human rights protection is its implementation by specific legal methods that are subject to strict regulation by means of provisions of existing normative legal acts or, in any case, they do not contradict them, and in addition, should not go beyond ethical norms and principles.
Vertical study of online reputation tools to measure public relations and communication activity
Authors: Compte-Pujol Marc , Sueldo Mariana , Cuenca-Fontbona Joan 
Published: November 2023

Introduction: Since the 1960s the interest in measuring the function of public relations and corporate communication has noticeably increased, thus the emergence of proposals for measurement indicators simply followed suit. Some models that explored this activity have focused on analyzing the relationships established between an organization and its publics, while others have explored the concept of corporate reputation. Currently, this function is also monitored in social networks.
Method and Analysis: The present study has delved into the structure of online reputation metrics listed by famous market research company Forrester that regularly publishes the state of the art for these types of tools (Liu, 2020; 2021). Delving further into previous descriptive research (Cuenca Fontbona, Matilla & Compte-Pujol, 2016), a descriptive study has been carried out of the websites of the organisations offering these metrics or "social listening platforms". Additional primary data has been collected through qualitative semi-structured interviews with communication professionals (N=180) from Lithuania in organizations of various sectors, in order to explore the level of awareness, adoption and usefulness of these online measurement tools for strategic decision making.
Results: It can be firmly claimed that all these digital monitors stem from a tradition set on the construct of reputation, while relationships, the very essence of the public relations profession, are excluded. Besides, there is an enormous confusion of terminology and criteria about the variables used; the lack of a universally valid instrument is evident. The value proposition includes some type of exercise related to public relations and corporate communication, yet the “online” corporate reputation metrics fail to encompass the extensive multidisciplinary area of ​​action that these disciplines display.
Conclusion: Although the management of intangible resources is a benchmark, these tools only provide quantitative information about the reputational image of a single stakeholder: consumers/customers, focusing on drivers related to the marketing and commercialization of the company’s products. Thus, they fail to provide comprehensive information on the reality of an organization’s corporate reputation.
Age Prevalence and Management of Breast Fibroadenoma at a Nigerian Tertiary Hospital: a Five-year (2016-2020) Analytical Review
Authors: Ogochukwu Anaziah Grace , Bolaji Gideon 
Published: November 2023

Abstract
Background: Fibroadenomas are one of the most common benign tumors of the breast in women between 18-39 years of age. In the adolescent population, the overall incidence of fibroadenoma is 2.2% and accounts for 68% of all breast masses. It feels like a smooth lump in the breast. It is painless and moves easily when touched.
Method: Following the consecutive percutaneous needle or excision surgical biopsy all breast masses were grossed and processed according to the standard histological tissue processing techniques. 241 of 1038 breast tissues were diagnosed of fibroadenoma in the Nigerian tertiary hospital.
Result: Out of 82% of histology specimen received for the period of five years, 13% were excised breast masses and 5% was confirmed positive for fibroadenoma. Out of this 5% of positive cases from 2016 to 2020, there was a slight and irregular interval of 1-4% increase each year. The prevalence of fibroadenoma was mostly seen in youths of ages 18-39 years having 75%, followed by adolescence of ages <18 years with 22% and lastly older adults of ages 40-59 years with 3%. The cause of this prevalence is not confirmed but with the current view of fibroadenoma cases in this study it can be predicted that there might be subsequent increase of cases in of this specific age group in the future.
The slight annual increase indicates that breast fibroadenoma across the varying age groups underwent varying degrees of somatic mutations and harbored mutations in the exon (exon 2) of the mediator complex subunit 12 (MED12) gene of the stromal cells of the breast which led to the excessive proliferation.
Conclusion: The data presented showed high prevalence of fibroadenoma cases in ages 18-39 annually which indicates that these individuals in this age group experienced rapid instability of the estrogen or progesterone hormone either due to childbearing, abortion, or genetic hormonal abnormalities. This suggests that there might be increased rates of abortion occurring among this age group in Nigeria or lack of proper postnatal care in Nigeria tertiary hospitals. More clinical investigations need to be carried out to confirm these suspected reasons in Nigeria.