bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Arctic Rivers Project: Using an Equitable Co‐Production Framework for Integrating Meaningful Community Engagement and Science to Understand Climate Impacts
Authors: Herman‐Mercer Nicole , Andre Alestine , Buschman Victoria , Blaskey Dylan , Brooks Cassandra , Cheng Yifan , Combs Evelynn , Cozzetto Karen , Fitka Serena , Koch Joshua , Lawlor Aine , Moses Elizabeth , Murray Emily , Mutter Edda , Newman Andrew J. , Prince Charles , Salmon Patricia , Tlen Jenessa , Toohey Ryan , Williams Michael , Musselman Keith N. 
Published: November 2023

AbstractAs the Arctic and its rivers continue to warm, a better understanding of the possible future impacts on people would benefit from close partnership with Indigenous communities and scientists from diverse fields of study. We present efforts by the Arctic Rivers Project to conduct community‐engaged research to increase collective understanding of the historical and potential future impacts of climate change on rivers, fish, and Indigenous communities. Working in central to northern Alaska and the Yukon Territory in Canada, the project seeks to engage with Indigenous communities in ethical and equitable ways to produces science that is useful, useable, and used that may serve as an example for future research efforts. Toward this goal, we formed an Indigenous Advisory Council and together developed project‐specific knowledge co‐production protocols. This paper provides a novel model of design and implementation to co‐produce knowledge with communities across a large study domain.
Methane Source Attribution in the UK Using Multi‐Year Records of CH4 and δ13C
Authors: Woolley Maisch Ceres A. , Fisher Rebecca E. , France James L. , Lowry David , Lanoisellé Mathias , Bell Thomas G. , Forster Grant , Manning Alistair J. , Michel Sylvia E. , Ramsden Alice E. , Yang Mingxi , Nisbet Euan G. 
Published: November 2023

AbstractIsotopic measurements of atmospheric methane are valuable for the verification of bottom‐up atmospheric emissions inventories. The balance of sources in emissions inventories must be consistent with the δ13C‐CH4 isotopic record in the air. Long‐term records of both methane mole fraction and δ13C from five sites across the UK are presented, showing post‐2007 growth in CH4 and negative trend in δ13C, consistent with global background sites. Miller‐Tans analyses of atmospheric measurements identified that the δ13C signature of the methane source mix varied between −50.1 and −56.1‰, with less depleted δ13C signatures at sites receiving air from urban areas, consistent with an increased proportion of thermogenic sources. Isotopic signatures calculated for all sites are more enriched than those expected from the bottom‐up emissions inventory, suggesting that inventories for the UK either underestimate contributions of thermogenic/pyrogenic emissions or overestimate biogenic sources.
Bulk CsPbClxBr3-x (1 ≤ x ≤ 3) perovskite nanocrystals/polystyrene nanocomposites with controlled Rayleigh scattering for light guide plate
Authors: Liu Chongming , Zhu Zhicheng , Pan Kaibo , Fu Yuan , Zhang Kai , Yang Bai 
Published: November 2023

AbstractPerovskite nanocrystals (PNCs)/polymer nanocomposites can combine the advantages of each other, but extremely few works can achieve the fabrication of PNCs/polymer nanocomposites by bulk polymerization. We originally adopt a two-type ligand strategy to fabricate bulk PNCs/polystyrene (PS) nanocomposites, including a new type of synthetic polymerizable ligand. The CsPbCl3 PNCs/PS nanocomposites show extremely high transparency even the doping content up to 5 wt%. The high transparency can be ascribed to the Rayleigh scattering as the PNCs distribute uniformly without obvious aggregation. Based on this behavior, we first exploit the potential of PNCs to serve as scatters inside light guided plate (LGP), whose surface illuminance and uniformity can be improved, and this new kind of LGP is compatible with the advanced liquid crystal display technology. Thanks to the facile composition adjustment of CsPbClxBr3-x (1 ≤ x ≤ 3) PNCs, the Rayleigh scattering behavior can also be adjusted so as to the performance of LGP. The best-performing 5.0-inch LGP based on CsPbCl2.5Br0.5 PNCs/PS nanocomposites shows 20.5 times higher illuminance and 1.8 times higher uniformity in display than the control. The LGP based on PNCs/PS nanocomposite exhibits an enormous potential in commercialization no matter based on itself or combined with the LGP-related technology.
Green wall system coupled with slow sand filtration for efficient greywater management at households
Authors: Yadav Ravi K. , Sahoo Siddhant , Yadav Asheesh K. , Patil Sunil A. 
Published: November 2023

AbstractGreen walls are gaining attention for greywater management in the imminent terrestrial space and land constraint scenario. They have been tested primarily with greywater from a single source such as showers, hand or wash basins, laundry, and kitchen or a mix of a couple of these sources but barely with mixed greywater from all these household activities. Here, a green wall system coupled with a slow sand filter (SSF) was tested for managing household greywater. It consisted of a set of five serial hydraulic flow-connected reactors and an SSF unit. Each reactor housed an Epipremnum aureum sapling embedded in the support bed matrix, consisting of cocopeat and granular activated charcoal. The system operated at 150 cm d−1 hydraulic loading rate (HLR) achieved 90 ± 0.7%, 85 ± 4.5%, 72.9 ± 4.4%, and 60.6 ± 5.1% removal efficiencies for turbidity, chemical oxygen demand (COD), total nitrogen (TN), and total phosphorous (TP), respectively. The system maintained similar treatment performance with varying greywater strength when COD and TN were below ~400 and ~15 mg L−1, respectively. The polished effluent produced by SSF operated at 187 cm d−1 HLR, with characteristics <5 mg L−1 COD, <2 NTU turbidity, <1 mg L−1 TN, ~0.5 mg L−1 TP, ~7.8 pH, and <100 MPN per 100 ml fecal coliforms, qualifies the standards for non-potable reuse applications. Along with reclaimed water reuse, green walls provide environmental benefits by fixing CO2 in plant biomass. Overall, the low-cost system offers efficient greywater management in an eco-friendly way with minimized resource consumption and areal footprint.
Mouse genome rewriting and tailoring of three important disease loci
Authors: Zhang Weimin , Golynker Ilona , Brosh Ran , Fajardo Alvaro , Zhu Yinan , Wudzinska Aleksandra M. , Ordoñez Raquel , Ribeiro-dos-Santos André M. , Carrau Lucia , Damani-Yokota Payal , Yeung Stephen T. , Khairallah Camille , Vela Gartner Antonio , Chalhoub Noor , Huang Emily , Ashe Hannah J. , Khanna Kamal M. , Maurano Matthew T. , Kim Sang Yong , tenOever Benjamin R. , Boeke Jef D. 
Published: November 2023

AbstractGenetically engineered mouse models (GEMMs) help us to understand human pathologies and develop new therapies, yet faithfully recapitulating human diseases in mice is challenging. Advances in genomics have highlighted the importance of non-coding regulatory genome sequences, which control spatiotemporal gene expression patterns and splicing in many human diseases1,2. Including regulatory extensive genomic regions, which requires large-scale genome engineering, should enhance the quality of disease modelling. Existing methods set limits on the size and efficiency of DNA delivery, hampering the routine creation of highly informative models that we call genomically rewritten and tailored GEMMs (GREAT-GEMMs). Here we describe ‘mammalian switching antibiotic resistance markers progressively for integration’ (mSwAP-In), a method for efficient genome rewriting in mouse embryonic stem cells. We demonstrate the use of mSwAP-In for iterative genome rewriting of up to 115 kb of a tailored Trp53 locus, as well as for humanization of mice using 116 kb and 180 kb human ACE2 loci. The ACE2 model recapitulated human ACE2 expression patterns and splicing, and notably, presented milder symptoms when challenged with SARS-CoV-2 compared with the existing K18-hACE2 model, thus representing a more human-like model of infection. Finally, we demonstrated serial genome writing by humanizing mouse Tmprss2 biallelically in the ACE2 GREAT-GEMM, highlighting the versatility of mSwAP-In in genome writing.
Glioma synapses recruit mechanisms of adaptive plasticity
Authors: Taylor Kathryn R. , Barron Tara , Hui Alexa , Spitzer Avishay , Yalçin Belgin , Ivec Alexis E. , Geraghty Anna C. , Hartmann Griffin G. , Arzt Marlene , Gillespie Shawn M. , Kim Yoon Seok , Maleki Jahan Samin , Zhang Helena , Shamardani Kiarash , Su Minhui , Ni Lijun , Du Peter P. , Woo Pamelyn J. , Silva-Torres Arianna , Venkatesh Humsa S. , Mancusi Rebecca , Ponnuswami Anitha , Mulinyawe Sara , Keough Michael B. , Chau Isabelle , Aziz-Bose Razina , Tirosh Itay , Suvà Mario L. , Monje Michelle 
Published: November 2023

AbstractThe role of the nervous system in the regulation of cancer is increasingly appreciated. In gliomas, neuronal activity drives tumour progression through paracrine signalling factors such as neuroligin-3 and brain-derived neurotrophic factor1–3 (BDNF), and also through electrophysiologically functional neuron-to-glioma synapses mediated by AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors4,5. The consequent glioma cell membrane depolarization drives tumour proliferation4,6. In the healthy brain, activity-regulated secretion of BDNF promotes adaptive plasticity of synaptic connectivity7,8 and strength9–15. Here we show that malignant synapses exhibit similar plasticity regulated by BDNF. Signalling through the receptor tropomyosin-related kinase B16 (TrkB) to CAMKII, BDNF promotes AMPA receptor trafficking to the glioma cell membrane, resulting in increased amplitude of glutamate-evoked currents in the malignant cells. Linking plasticity of glioma synaptic strength to tumour growth, graded optogenetic control of glioma membrane potential demonstrates that greater depolarizing current amplitude promotes increased glioma proliferation. This potentiation of malignant synaptic strength shares mechanistic features with synaptic plasticity17–22 that contributes to memory and learning in the healthy brain23–26. BDNF–TrkB signalling also regulates the number of neuron-to-glioma synapses. Abrogation of activity-regulated BDNF secretion from the brain microenvironment or loss of glioma TrkB expression robustly inhibits tumour progression. Blocking TrkB genetically or pharmacologically abrogates these effects of BDNF on glioma synapses and substantially prolongs survival in xenograft models of paediatric glioblastoma and diffuse intrinsic pontine glioma. Together, these findings indicate that BDNF–TrkB signalling promotes malignant synaptic plasticity and augments tumour progression.
Neural signal propagation atlas of Caenorhabditis elegans
Authors: Randi Francesco , Sharma Anuj K. , Dvali Sophie , Leifer Andrew M. 
Published: November 2023

AbstractEstablishing how neural function emerges from network properties is a fundamental problem in neuroscience1. Here, to better understand the relationship between the structure and the function of a nervous system, we systematically measure signal propagation in 23,433 pairs of neurons across the head of the nematode Caenorhabditis elegans by direct optogenetic activation and simultaneous whole-brain calcium imaging. We measure the sign (excitatory or inhibitory), strength, temporal properties and causal direction of signal propagation between these neurons to create a functional atlas. We find that signal propagation differs from model predictions that are based on anatomy. Using mutants, we show that extrasynaptic signalling not visible from anatomy contributes to this difference. We identify many instances of dense-core-vesicle-dependent signalling, including on timescales of less than a second, that evoke acute calcium transients—often where no direct wired connection exists but where relevant neuropeptides and receptors are expressed. We propose that, in such cases, extrasynaptically released neuropeptides serve a similar function to that of classical neurotransmitters. Finally, our measured signal propagation atlas better predicts the neural dynamics of spontaneous activity than do models based on anatomy. We conclude that both synaptic and extrasynaptic signalling drive neural dynamics on short timescales, and that measurements of evoked signal propagation are crucial for interpreting neural function.
MTHFR C677T and A1298C polymorphism’s effect on risk of colorectal cancer in Lynch syndrome
Authors: Wiik Mariann Unhjem , Negline Mia , Beisvåg Vidar , Clapham Matthew , Holliday Elizabeth , Dueñas Nuria , Brunet Joan , Pineda Marta , Bonifaci Nuria , Aretz Stefan , Klinkhammer Hannah , Spier Isabel , Perne Claudia , Mayr Andreas , Valle Laura , Lubinski Jan , Sjursen Wenche , Scott Rodney J. , Talseth-Palmer Bente A. 
Published: November 2023

AbstractLynch syndrome (LS) is characterised by an increased risk of developing colorectal cancer (CRC) and other extracolonic epithelial cancers. It is caused by pathogenic germline variants in DNA mismatch repair (MMR) genes or the EPCAM gene, leading to a less functional DNA MMR system. Individuals diagnosed with LS (LS individuals) have a 10–80% lifetime risk of developing cancer. However, there is considerable variability in the age of cancer onset, which cannot be attributed to the specific MMR gene or variant alone. It is speculated that multiple genetic and environmental factors contribute to this variability, including two single nucleotide polymorphisms (SNPs) in the methylenetetrahydrofolate reductase (MTHFR) gene: C677T (rs1801133) and A1298C (rs1801131). By decreasing MTHFR activity, these SNPs theoretically reduce the silencing of DNA repair genes and increase the availability of nucleotides for DNA synthesis and repair, thereby protecting against early-onset cancer in LS. We investigated the effect of these SNPs on LS disease expression in 2,723 LS individuals from Australia, Poland, Germany, Norway and Spain. The association between age at cancer onset and SNP genotype (risk of cancer) was estimated using Cox regression adjusted for gender, country and affected MMR gene. For A1298C (rs1801131), both the AC and CC genotypes were significantly associated with a reduced risk of developing CRC compared to the AA genotype, but no association was seen for C677T (rs1801133). However, an aggregated effect of protective alleles was seen when combining the alleles from the two SNPs, especially for LS individuals carrying 1 and 2 alleles. For individuals with germline pathogenic variants in MLH1, the CC genotype of A1298C was estimated to reduce the risk of CRC significantly by 39% (HR = 0.61, 95% CI 0.42, 0.89, p = 0.011), while for individuals with pathogenic germline MSH2 variants, the AC genotype (compared to AA) was estimated to reduce the risk of CRC by 26% (HR = 0.66, 95% CI 0.53, 0.83, p = 0.01). In comparison, no association was observed for C677T (rs1801133). In conclusion, our study suggests that combining the MMR gene information with the MTHFR genotype, including the aggregated effect of protective alleles, could be useful in developing an algorithm that estimates the risk of CRC in LS individuals.
Performance of serum soluble interleukin-2 receptor as a diagnostic marker for lymphoma in patients with fever
Authors: Kanda Naoki , Yamaguchi Ryota , Yamamoto Yu , Matsumura Masami , Hatakeyama Shuji 
Published: November 2023

AbstractThere have been few reports on the diagnostic performance of soluble interleukin-2 receptor (sIL-2R) for lymphoma. A cross-sectional study was conducted at a university hospital; all patients who were admitted to the Division of General Internal Medicine and underwent serum sIL-2R testing were included. Patients were divided into two groups based on the presence of fever (≥ 38.0 °C). Among 602 patients, 421 had fever and 76 were diagnosed with lymphoma (48 of the 76 were in the febrile group). In all patients, the area under the receiver operating characteristic curve (AUROC) of sIL-2R for the diagnosis of lymphoma was 0.81 [95% confidence interval (CI), 0.75–0.87]. The AUROC was significantly higher in the febrile group (0.88; 95% CI, 0.81–0.94) than in the afebrile group (0.75; 95% CI, 0.65–0.85). In the febrile group, the sensitivity and specificity were 81.2% and 82.3%, respectively, with an optimal cutoff value of 3,250 U/mL. In the afebrile group, they were 89.3% and 54.9%, respectively, with a cutoff value of 868 U/mL. Serum sIL-2R showed high performance as an adjunctive diagnostic marker for lymphoma, particularly among febrile patients. Different cutoff values should be used for patients with and without fever to maximize diagnostic performance.
Technical efficiency and its determinants in regulating adolescents’ coronavirus infection across Asian countries
Authors: Maity Shrabanti , Sinha Anup 
Published: November 2023

AbstractThe coronavirus pandemic, besides generating health distress, influences the socio-economic conditions of humankind. Every adolescent's lifestyle is affected by the virus. Healthy adolescents are not only key contributors to the forthcoming workforce but also a source of a country’s human capital. The purpose of the article is to examine the efficacy of various Asian countries in regulating the spread of the coronavirus among adolescents. In addition to that, the paper also attempts to pinpoint the prime causes of the inefficiency of a country in regulating the same. The paper also examines the behavioural changes among adolescents across Asian countries in pre-and-post pandemic times. In this context, the study identifies the impact of adolescents’ tobacco consumption, female political leadership, and accreditation on a country’s efficacy to regulate adolescents’ coronavirus infection. The study’s empirical analysis covers twenty-one Asian countries. By using the Panel Stochastic Production Frontier, the study concludes that Kazakhstan is the most efficient country and Afghanistan is the least efficient country on the list. The inefficiency effects estimates conclude that adolescents’ tobacco consumption decreases and good governance practices increase the efficiency of a country in regulating the spread of adolescent coronavirus infection. Additionally, the paper finds no significant behavioural changes among adolescents in pre-and-post pandemic times across Asian countries. The paper concludes with appropriate policy recommendations supported by empirical evidence. The paper also identifies its shortcomings and suggests potential future lines of inquiry.
Optimized scheduling study of user side energy storage in cloud energy storage model
Authors: Wang Huidong , Yao Haiyan , Zhou Jizhou , Guo Qiang 
Published: November 2023

AbstractWith the new round of power system reform, energy storage, as a part of power system frequency regulation and peaking, is an indispensable part of the reform. Among them, user-side small energy storage devices have the advantages of small size, flexible use and convenient application, but present decentralized characteristics in space. Therefore, the optimal allocation of small energy storage resources and the reduction of operating costs are urgent problems to be solved. In this study, the author introduced the concept of cloud energy storage and proposed a system architecture and operational model based on the deployment characteristics of user-side energy storage devices. Additionally, a cluster scheduling matching strategy was designed for small energy storage devices in cloud energy storage mode, utilizing dynamic information of power demand, real-time quotations, and supply at the load side. Subsequently, numerical analysis was conducted to verify that the proposed operational mode and optimal scheduling scheme ensured the maximum absorption of renewable energy, improved the utilization rate of energy storage resources at the user side, and contributed to peak shaving and load leveling in the power grid. The model put forward in this study represents a valuable exploration for new scenarios in energy storage application.
Effectiveness of probiotics and clove essential oils in improving growth performance, immuno-antioxidant status, ileum morphometric, and microbial community structure for heat-stressed broilers
Authors: Elbaz Ahmed M. , Ashmawy Eman S. , Ali Safaa A. M. , Mourad Disouky M. , El-Samahy Hanan S. , Badri Faisal B. , Thabet Hany A. 
Published: November 2023

AbstractRecently, interest has increased in using bio-additives, herbs, and their extracts as feed additives because of their potential role in improving chick's health and productivity, especially during stress. Thus, our aim in this study is to examine whether nutritional supplementation (probiotics and clove essential oils) will help mitigate the negative effect of heat stress on the bird by modifying the microbial content, boosting immunity, oxidative status, metabolic, and growth. In this study, three hundred one-day-old broiler chicks (Ross 308) were fed the following experimental diet: (CON) basal diet (control diet); (CEO) CON with clove essential oils (300 mg/kg); (PRO) CON with probiotics (2 g/kg); (PC) CON with probiotics and clove essential oils. Our results showed a significant improvement (P < 0.05) in body weight gain, feed conversion ratio, nutrient digestibility, and digestive enzymes activities in broilers fed on PC, CEO, and PRO compared to the control group. Moreover, a significant decrease was recorded in the abdominal fat content and an increase in the relative weight of bursa of Fabricius, and higher antibody levels against Newcastle disease virus, as well as, there was an increase (P < 0.05) in interleukin 10 (IL-10) in all treated groups. Meanwhile, there was a decrease in tumor necrosis factor-α (TNF-α) in all supplemented groups compared with the control group. Serum triglycerides, cholesterol, low-density lipoprotein concentrations, and alanine aminotransferase activities were significantly lower in the treated groups. Superoxide dismutase and glutathione peroxidase levels were elevated (P < 0.05) and the malondialdehyde level value significantly decreased in all supplemented groups. The treated groups enhanced the ileum structure by increasing Lactobacillus, decreasing E. coli, and improving the morphometrically (P < 0.05). This study strongly suggests that clove essential oil and probiotic mixture can be used as a feed supplement to reduce the effects of heat stress by improving the growth performance and enhancing immuno-antioxidant status, ileum morphometric, as well as modifying the microbial community structure of the ileum of broilers.
Essentiality of Nfatc1 short isoform in osteoclast differentiation and its self-regulation
Authors: Omata Yasuhiro , Tachibana Hideyuki , Aizaki Yoshimi , Mimura Toshihide , Sato Kojiro 
Published: November 2023

AbstractDuring osteoclast differentiation, the expression of the transcription factor nuclear factor of activated T cell 1 (Nfatc1) increases in an autoproliferative manner. Nfatc1 isoforms are of three sizes, and only the short isoform increases during osteoclast differentiation. Genetic ablation of the whole Nfatc1 gene demonstrated that it is essential for osteoclastogenesis; however, the specific role of the Nfatc1 short form (Nfatc1/αA) remains unknown. In this study, we engineered Nfatc1 short form-specific knockout mice and found that these mice died in utero by day 13.5. We developed a novel osteoclast culture system in which hematopoietic stem cells were cultured, proliferated, and then differentiated into osteoclasts in vitro. Using this system, we show that the Nfatc1/αA isoform is essential for osteoclastogenesis and is responsible for the expression of various osteoclast markers, the Nfatc1 short form itself, and Nfatc1 regulators.