bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modified Kasai operation combined with autologous bone marrow mononuclear cell infusion for biliary atresia
Authors: Nguyen ThanhLiem , Nguyen Hoang - Phuong , Phan Thi Kieu Trang , Ngo Duy Minh , Ha Thi Thu Hien , Bui Thi Hang , Nguyen Thanh Quang 
Published: October 2023

Abstract

Aim: To evaluate the safety and outcomes of modified Kasai operation combined with autologous bone marrow mononuclear cell (BMMNC) infusion for biliary atresia (BA).
Methods: A prospective study was performed from January 2015 to December 2021. Ten consecutive children with BA who underwent modified Kasai operation combined with autologous BMMNC infusion were included. Kasai operation was performed with two modifications: the liver was exteriorized partially, and two hepatic pedicles were encircled and retracted laterally.
Bone marrow was harvested through anterior iliac crest puncture under general anesthesia then a modified Kasai operation was performed. After processing, bone marrow mononuclear cells were infused through the umbilical vein at the end of the operation.
Serum bilirubin, albumin, alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, and prothrombin time were monitored at baseline, six months, twelve months, and the last follow-up (4.5 years) after the operation. In addition, esophagoscopy and liver biopsies were performed on patients whose parents agreed. Mixed-effects analysis was used to evaluate the changes in Pediatric End-Stage Liver Disease (PELD) scores.
Results: There were no intraoperative or postoperative complications related to the operation or cell infusion. The average infused BMMNC and CD34+ cell counts per kg bodyweight were 85.5 ± 56.0 x 106/kg and 10.0 ± 3.6 x 106 for the injection, respectively. After the intervention, all ten patients survived with a mean follow-up duration of 4.5 ± 0.9 years. Liver function was maintained or improved after the operation and cell infusion, as assessed by biochemical tests. The severity of the disease reduced markedly, with a significant reduction in PELD scores.
Conclusion: Autologous BMMNC administration combined with Kasai operation for BA is safe and may maintain or improve liver function. A randomized study with a control group should be performed to provide accurate conclusions on the efficacy of this approach.
ClinicalTrials.gov Identifier: NCT05517317 on August 26th, 2022.
Does centralized dispensing and distribution of medicines improve health outcomes? A retrospective cohort study in Ndola, Zambia
Authors: Molaudi Gaula , Constance Wose Kinge , Tawanda Dube , Ivin Chibanda , Thapelo Maotoe , Francis Akpan , Shabir Banoo , Eula Mothibi , Fanie Hendriksz , Ian Sanne , Ben Chirwa , Charles Chasela 
Published: October 2023

Abstract

Background
The global shift toward universal test and treat policy for individuals living with HIV has triggered an unprecedented surge in healthcare demand. In response, Zambia's government implemented a centralized dispensing and distribution intervention. Despite successful implementation, empirical evidence of its impact within Zambia remains lacking. This study aims to assess this intervention's utilization patterns and measure its effect on medicine collection rates, retention, and plasma viral load levels.
Methods
We conducted a retrospective cohort study by analysing programmatic data from 17,157 stable patients who received chronic antiretroviral therapy through the intervention across nine high-volume treatment facilities in Ndola, Zambia, from January 2018 to September 2021. We computed the annual ratios of the key health outcomes.
Results
Between January 2018 and September 2021, a total of 17,157 clients were enrolled, 75% of the total target during the implementation period. Out of those enrolled, there were more females 69% than males 31%, with most clients aged 20 years and above. The yearly rates of beneficiary medicine collection and retention consistently surpassed the benchmark, reaching 93% (90% CI: 85% − 100%) and 97% (95% CI: 95% – 99%), respectively. However, less than 1% of those ever-enrolled experienced escalated viral load levels warranting discontinuation from the program.
Conclusion
Our study establishes that implementing the centralized dispensing and distribution intervention in Ndola produced a notable positive impact on enrolled beneficiaries' critical health outcomes. This reinforces the intervention's viability as a scalable solution, holding potential for broader population-level benefits.
Cashless payment methods and tax evasion: Evident in 28 countries in Europe and Vietnam - Looking at Vietnam Economic Policies Dealing with Cashless Payment Methods
Authors: Tran Loan , Wang Wen Pu 
Published: October 2023

Abstract
A cashless society has numerous advantages such as lower financial transaction costs and fewer tax evasion concerns. Cashless payment methods can mitigate tax evasion because they leave a trail of records of the transactions. Using panel data from 29 countries from 2000 to 2021, this study investigated the connection between different cashless payment methods and tax compliance, analyzed the connection between each cashless payment method in the context of the value-added tax, and synthesized the effect of different variables affecting tax evasion. Furthermore, this study also looked into Vietnam’s current practices and implementations of policies governing cashless payment methods as well as its desire to transition into an e-Government; this study looks at the current realities of Vietnam’s efforts into this endeavor and provides recommendations and directions for the implementation of the cashless payment system as well as the transition into an e-Government.
Effect of bacterial secretomes isolated from larval food of Frieseomelitta varia on Drosophila melanogaster model for Azheimer's Disease
Authors: Sales Michelle Ribeiro , Bonetti Ana Maria , Rodrigues Tamiris Sabrina , Vieira Carlos Ueira , Malta Serena Mares , Santos Ana Carolina Costa 
Published: October 2023

Abstract

Alzheimer's disease (AD) is the most prevalent neurodegenerative pathology in Brazil. It is associated with age and cognitive and neuropsychiatric manifestations, which result in progressive and disabling disability. It is responsible for 60–70% of dementia cases with an estimated increase of 10 million diagnoses annually. Research in AD suggests that the imbalance between the production and elimination of amyloid beta (Aβ) (amyloid hypothesis) is responsible for events that lead to AD. The present study investigated the neuroprotective effect and inhibitory activity of the bacterial secretome isolated from the larval food of the stingless bee Frieseomelitta varia in a transgenic line of Drosophila melanogaster, a model for the study of AD. This has been an important model organism for research into the events involved in AD. DA model flies were treated with different bacterial secretomes for 15 days after eclosion. Secretomes identified as S27 and S40 improved the climbing rate of these flies, assessed through Rapid Interactive Negative Geotaxis (RING). Histological analysis of the brain revealed potential for decreased neurodegeneration. Therefore, the bacterial secretome isolated from stingless bee larval food showed potential as a source of bioactive molecules to reduce the neurodegeneration process in AD.
Synthesis, characterization, and investigation of pharmacological studies of Some nanoparticles
Authors: Youssef Fady Sayed , Ismail Sameh Hamed , ElHalim Hanan F. Abd , Mohamed Gehad G. 
Published: October 2023

Abstract
In this paper, a rapid, facile, and cheap method is used to synthesize gold and titanium dioxide nanogel. Cytotoxicity, invitro anti-inflammatory and antioxidant activities were performed. Invitro antimicrobial activities with molecular docking confirmation were carried out. Also, wound healing was performed in rabbit model with H & E illustration. Characterization and identification of the prepared compounds were investigated using X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and atomic force microscope (AFM). XRD illustrated the presence of the characteristic peaks of the gold and rutile. DLS revealed the homogeneity in size for both gold and rutile nanogel. Where the size of gold/carbopol nanogel and titanium dioxide/carbopol nanogel were 32 nm and 51 nm, respectively, with a zeta-potential of +22 and -28 mV, respectively. BET surface area was found to be 43 and 34 m2/g for gold/carbopol nanogel and titanium dioxide/carbopol nanogel, respectively. The data collected from TEM, SEM, and AFM images studies illustrated the spherical to subspherical shape of gold//carbopol nanogel and titanium dioxide/carbopol nanogel. Cytotoxicity of titanium dioxide/carbopol nanogel and gold/carbopol nanogel on normal human diploid cell line (WI-38 cell) depending on (MTT protocol) and IC50 values were found to be 302.96 and 382.99 ug/ml for titanium dioxide/carbopol nanogel and gold/carbopol nanogel, respectively. Molecular docking with Microbial Beta-Ketoacyl-ACP Synthase II complexed with Malonyl-COA was also presented. The docking score of gold/carbopol nanogel was -3.20 (kcal/mol), however, titanium dioxide/carbopol nanogel was -2.78 (kcal/mol). The anti-microbial effect against ATCC 6538 and ATCC 10221 microorganisms was described and evaluated for the prepared NPs. The MIC50 values recorded were 5 µg/mL and 20 µg/mL for gold/carbopol nanogel and titanium dioxide/carbopol nanogel, respectively. These results confirmed the superior potential antimicrobial effect of gold/carbopol nanogel than titanium dioxide/carbopol nanogel. The antioxidant activity was studied by the DPPH method. The obtained results showed that both synthesized compounds have a potent ability to scavenge hydrogen peroxide when compared to traditional vitamin C. Results of invitro anti-inflammatory activity revealed the potential and superior effect of gold/carbopol nanogel than titanium dioxide/carbopol nanogel. Results of wound healing study revealed the potential and superior efficacy of gold/carbopol nanogel than titanium dioxide/carbopol nanogel.
Isolation and characterization of Lactic Acid Bacteria from ‘Pakkat su’- A traditional fermented food of the Tai Phake community of Assam
Authors: sarmah minakshee , Deka Manab 
Published: October 2023

Abstract

Background
Lactic Acid Bacteria (LAB) plays an important role in the fermentation of traditional food of ethnic communities across the globe. This study isolated and identified LAB from ‘Pakkat su’, vegetable-based fermented food of the Tai Phake community of Assam. Pure microbial cultures were screened for their probiotic properties, viz., tolerance to acid and bile, bile salt hydrolysis, resistance against antibiotics, antimicrobial and hemolytic activities, and cell surface characteristics.
Result
Three isolates from pure cultures showed bile, pH and bile tolerance and non-hemolytic and non-pathogenic properties. Antagonistic effects of these isolates were observed against E.coli ATCC-10536 and Staphylococcus aureus ATCC-BAA-1026. These potent bacterial isolates were identified as new strains of Lactiplantibacillus plantarum, Lactobacillus casei, and Lactiplantibacillus pentosus based on 16SrRNA sequences which were submitted to GenBank database with sequential accession number OQ996876, OQ997380 and OQ998330.
Conclusion
These results indicate that microbes associated with ‘Pakkat su’ production are good candidates for probiotic production and can be considered for potential application as commercial probiotic to confer health benefits to consumers. It also implies that ‘pakkat su’ can be used as vehicles for probiotic delivery.
Microglial Piezo1-mediated mechanotransduction play an important role in activation of neurotoxic reactive astrocyte in experimental glaucoma
Authors: Zhang Jingxue , Wu Shen , Cheng Ying , Liu Qian , Yan Xuejing , Ren Tianmin , Wang Ningli 
Published: October 2023

Abstract
Background:
Previous studies indicated that activation of glial cells and inflammatory responses are triggering factors of glaucomatous optic neuropathy. In particular, neurotoxic reactive astrocytes have been found to play a significant role in retinal ganglion cell damage. Furthermore, research showed that activation of glial cells is not dependent on retinal ganglion cell injury, but rather a direct response to pathological elevation of intraocular pressure (IOP). However, the molecular mechanism by which elevated IOP triggers glial activation through mechanical forces remains unknown.
Main body:
We constructed a high IOP animal model by injecting Matrigel hydrogel into the anterior chamber of rats. The spatial transcriptomic sequencing and bioinfomation analysis were performed using tissues from the high IOP rats and control rats. Subsequently, mechanical intervention was given to cultured microglia in vitro, and the conditioned medium after intervention was applied to astrocytes to explorethe interaction between microglia and astrocytes under mechanical stimulation, and QPCR and Western Blot examinations were used to analyze the changes in secretory factors of microglia under mechanical stimulation. The role of piezo1 as a mechanical receptor in microglia was further clarified by activating or inhibiting piezo1 in the cultured microglia under mechanical stimulation. By spatial transcriptome sequencing, we determined for the first time in vivo that glial cells in optic nerve head were rapidly activated, and the neurotoxic reactive astrocyte were induced under high IOP. Subsequently, we demonstrated that microglial culture medium after mechanical intervention can significantly promote the transformation of astrocytes into neurotoxic astrocytes, and the key regulatory factors are inflammatory factors such as IL-1α and TNF-α. In addition, we found that microglia activate and up-regulate the expression of these inflammatory factors by sensing mechanical stimulation mainly through piezo1.
Conclusions:
Our study revealed that the neurotoxic astrocyte would rapidly be reactivated in the optic nerve head under elevated intraocular pressure in vivo. Additionally, we found that microglia could be activated by directly sensing mechanical force via Piezo1, resulting in the release of key factors such as IL-1α and TNF-α, which is an important triggering mechanism for the formation of neurotoxic reactive astrocyte.
Study on microstructure characterization and mechanical properties of AISI 444 Ferritic Stainless Steel Joint by high frequency pulse K-TIG welding
Authors: Dong Zhihai , Tian Ye , Hao Tingting , Li YiWen , Miu Junyan , Cui XinYing , Chang Chenhe , Chang Yunlong 
Published: October 2023

Abstract
In this study, high frequency pulse K-TIG was used to weld AISI 444 ferritic stainless steel (FSS) with a thickness of 6 mm, and the welding process window was determined, and complete penetration was achieved in the state of single pass autogenous welding. The influence law of technological parameters and weld appearance was analyzed. The microstructure characteristics of weld zone (WZ) and heat-affected zone (HAZ) was analyzed. Finally, the mechanical properties of welded joints are evaluated. It is found that the thermal conductivity of FSS is large, so the welding process window is relatively narrow, and the welding heat input determines whether the weld can achieve complete penetration.
The second phase, such as σ phase, χ phase and Laves phase, is precipitated in WZ and HAZ phases. At the crystal boundary, the σ phase and χ phase appears, while the Laves phase is dispersed in the intragranular and crystal boundary.
In addition, NbC isfound in the HAZ. The brittle-hard phase and coarse grain have great influence on tensile properties and impact toughness at room temperature. The tensile strength of the weld is equivalent to 70.57% of the parent material, which is 596 MPa. Its average impact toughness value is 29.7J, which is equivalent to 43.04% of the BM. Although the strength and toughness of FSS joints welded with high-frequency pulse K-TIG are lower than that of BM, the welding efficiency is significantly improved, and it is suitable for components with slightly lower load requirements.
Alterations in DNA conformation and histone phosphorylation resulting from double-strand breaks induced in HeLa cells – a spectroscopic and immunostaining approach
Authors: Czaja Michał , Giergiel Magdalena , Skirlińska-Nosek Katarzyna , Sofińska Kamila , Adamczyk Olga , Lupa Dawid , Seweryn Sara , Rajfur Zenon , Birarda Giovanni , Piccirilli Federica , Vaccari Lisa , Szymoński Marek , Lipiec Ewelina 
Published: October 2023

Abstract

All living cells are subjected daily to several thousands of DNA lesions of various origins. DNA damage leads to genetic material disorders that may have serious consequences, including cell apoptosis or mitotic death. The most dangerous DNA lesions are double-strand breaks (DSBs) accompanied by modifications of the DNA chemical structure. Here, we focus on DNA conformational transitions caused by DSBs formation induced by a genotoxic drug, bleomycin (BLM). The response of cells to this anticancer agent treatment was monitored with synchrotron radiation infrared micro-spectroscopy (SR-FTIR) and confocal laser scanning microscopy (CLSM). Obtained data were analyzed using multivariate data analysis methods. Depending on BLM concentrations, either DNA repair or apoptosis was induced. The applied methodology enabled tracking of molecular modifications on those processes, such as conformational transition from B-DNA to A-DNA and increased expression of proteins within the cell nuclei resulting from the activation of repair processes. Finally, we present a novel approach to determine the rate of chromatin damage based on computational analysis of the histone H2AX phosphorylation immunoassay.
A Case Report of Multiple Systemic Immune Adverse Reaction Caused by Immune Checkpoint Inhibitors in Lung Cancer
Authors: Wang Mudan , Li Enci , Dong Shaoshao 
Published: October 2023

Abstract

Background: Immune checkpoint inhibitors(ICIs) have been shown to have antitumor activity in various solid tumors. With the increase in clinical applications of ICIs, reports of immune-related adverse events (irAEs) have also increased. ICI-associated renal adverse effects are rare, accompanying Multiple Systemic Immune Adverse Reaction.
This article reports a case of a lung cancer patient who developed multisystemic adverse effects after PD-1 inhibitor application: acute kidney injury, rash, thyroiditis, and pneumonia. Renal biopsy histopathological results acute tubulointerstitial nephritis. His multisystemic dysfunction recovered within 3 months of treatment with prednisolone in addition to discontinuation of tislelizumab.
Glucocorticoids are the main treatment, and close attention should be paid to the presence or absence of new irAEs during the process. Besides,Despite the patient’s eventual discontinuation of antitumor therapy due to severe irAEs, a significant and durable therapeutic response was observed
RUNX1-BMP2 Promotes Vasculogenic mimicry in Laryngeal Squamous Cell Carcinoma via Activation of the PI3K-AKT Signalling Pathway
Authors: Zhu Qingwen , Zhang Xinyu , Lu Fei , Miao Siyu , Zhang Chunyang , Liu Zhenzhen , Gao Zejun , Qi Meihao , An Xiaogang , Geng Panling , Han Fugen , Zhang Ruyue , Zha Dingjun 
Published: October 2023

Abstract
Background Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors of the head and neck. Vasculogenic mimicry (VM) refers to fluid channels formed by invasive tumour cells rather than endothelial cells and is crucial for tumour growth and metastasis. However, the regulatory mechanisms underlying VM during the malignant progression of LSCC remain largely unknown.Methods Gene expression and clinical data for LSCC were sourced from the TCGA and Gene GEO (GSE27020) databases. A risk prediction model associated with VM was established using LASSO and COX regression analyses. Based on their risk scores, patients with LSCC were categorised into high- and low-risk groups. The disparities in immune infiltration, tumor mutational burden (TMB), and functional enrichment between these two groups were examined. The identification of core genes in LSCC was conducted using the machine learning (SVM-RFE) and the WGCNA algorithm. Subsequently, the involvement of Bone Morphogenetic Protein 2 (BMP2) in VM and metastasis was investigated both in vitro and in vivo. To elucidate the downstream signaling pathways regulated by BMP2, Western blot experiments were performed. Additionally, ChIP experiments were employed to identify the key transcription factors responsible for modulating BMP2 expression.Results We established a new precise prognostic model for LSCC related to VM based on three genes: BMP2, EPO, and AGPS. The ROC curves from both TCGA and GSE27020 validation cohorts demonstrated precision survival prediction capabilities, with the nomogram showing some net clinical benefit. Multiple algorithm analyses indicated that BMP2 was a potential core gene. Further experimental results suggested that BMP2 promotes VM and metastasis in LSCC. The malignant progression of LSCC is promoted by BMP2 via the activation of PI3K-AKT signalling pathway, and the high expression of BMP2 in LSCC was a result of its transcriptional activation by runt-related transcription factor 1 (RUNX1).Conclusion BMP2 predicts poor prognosis in LSCC, promotes LSCC VM and metastasis through the PI3K-AKT signalling pathway, and is transcriptionally regulated by RUNX1. BMP2 may be a novel, precise diagnostic, and therapeutic biomarker for LSCC.
Differentiating benign and malignant neoplasms: A new role for coagulation and fibrinolysis indicators
Authors: Zhou Xuan , Zeng Lu , Liu Shuangfeng , Tang Na 
Published: October 2023

Abstract
Background
Abnormal coagulation and fibrinolysis are often observed in malignant tumors, which may contribute to tumor invasion. This study aimed to investigate the potential of using coagulation and fibrinolysis markers for differential diagnosis.
Methods
The levels of PT, APTT, TT, FIB, FDP, and D-dimer were analyzed in primary tumors (208 benign and 243 malignant) prior to biopsy or treatment. Additionally, a control group consisting of 400 healthy volunteers was included.
Results
Significantly higher levels of PT, FIB, FDP and D-dimer were found in the malignant tumor group compared with both the benign tumor group and control group (P > 0.01). The AUCs for FIB and D-dimer in diagnosing malignant tumors were 0.829(95% CI:0.780 ~ 0.878)and 0.845(95% CI:0.799 ~ 0.892), respectively. - both exceeding 0.8; their specificities for detecting malignancy were also high at 91.18% and 92.16%, respectively; while their positive predictive values reached up to 91.82% and 92.73%, respectively. Furthermore, the combined detection of PT,FIB,D-dimer,and FDP as well as that of only FIB and D-dimer combined could achieve an even higher diagnostic accuracy with AUCs were 89.22% and 89.26%, respectively;specificities for detecting malignancy were 89.22% and 89.26%,while their positive predictive values were 91.79% and 91.03%. Finally, a combination factor involving these markers could provide valuable assistance in distinguishing between benign and malignant tumors.
Conclusion
The use of FIB and D-dimer alone and the combined use of coagulation system function-related indicators (such as the combination of D-dimer and FIB or the combination of PT, FIB, FDP and D-dimer) have high auxiliary diagnostic value in the differential diagnosis of benign and malignant tumours.