bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of forensic genetics: ITO index distribution and kinship judgment between two individuals
Authors: Jiang Huanchang , Jiang Qinglian , Wu Shuzhen 
Published: December 2023

Abstract
According to Mendel’s law of genetic separation, there must be a certain blood relationship between members of the same family, and two individuals with blood relations must have the following three situations, that is, there are two homologous genes (I denotes), one homologous gene (T denotes), and no homologous genes (O denotes), which is the ITO index for calculating the blood relationship between two individuals. The AGGU Expressmarker 22 kit, ABI 3500 genetic analyzer, and GeneMapper ID-X v1.5 software were used to statistically analyze the ITO index of the gene locus of 5 kinds of samples, 28 pairs of monozygotic twins (MT), 4,000 pairs of parent–offspring (PO), 392 pairs of full sibling (FS), 138 pairs of half-siblings (HS) (including grandchildren, uncles, and nephews) and 3,500 pairs of unrelated individuals (UI). Observing the median distribution of ITO index found that from MT, PO, FS, HS to UI, the more distant the kinship, the smaller the ITO index. Full sibling index (FSI)/half-sibling index (HSI) ≥ 1 can be used as the FS discriminant standard, FSI/HSI < 1 can be used as the HS discriminant standard. According to the distance of kinship, from the direction of MT, PO, FS, HS, and UI, the proportion of the maximum ITO index of the same type of sample in the true kinship index item showed a decreasing trend. ITO index is an important statistical means to identify the kinship between two individuals, according to which the ITO index can accurately determine the kinship between individuals, which has high application value. MT index is not 0 to identify relatives as MT, PO index is an important indicator to distinguish between relatives as PO and FS. The critical values of ITO index discriminant values for UI and HS need to be further studied.
The Bullish Game: The Role of Virtual Currency in Incentivizing Homework on the Skyeng Platform
Authors: Адамович Ксения , Чиркина Татьяна 
Published: December 2023

Even though online learning is an affordable way to gain new knowledge and skills, and millions of people use different platforms, there is a serious problem with students dropping out, as well as with their completion of assignments during training. One effective way to increase student engagement is to use gamification in teaching. In this study, we study the relationship between the introduction of such a gamification element as virtual currency with the results of learning English on the Skyeng platform. Using data from 2019 to 2020, we analyzed the relationship between the indicated intervention and the percentage of completion of homework, as well as the average homework score of platform users.
The results of the experiment conducted in the A/B format showed that the introduction of virtual currency had a significant effect on the educational results of students. Thus, students from the experimental group showed an increase in involvement in doing homework on the platform compared to the control group. Moreover, various types of rewards work in general to increase student engagement in homework, including those that are not directly related to learning. At the same time, students who did not use the game currency available to them showed similar or even lower results than participants in the experiment from the control group. It can be assumed that in this case there is an effect of student motivation as a hidden factor, which explains the greater interest in the functionality and capabilities of the platform itself, and in the educational process as a whole.
Problems and methods of analysis of the russian-language texts by sentiment identification
Authors: Алтышева М.А. 
Published: December 2023

Проводится анализ актуальности изучения и разработок в области обработки естественно-языковых текстов (NLP), приводятся прогнозы аналитических сообществ, рассматриваются основные методы и подходы сентимент-анализа русскоязычных текстов. Отдельный блок посвящен проблемам исследования тональности русскоязычных текстов и глобальным проблемам, с которыми сталкиваются ученые во всем мире вне зависимости от языка.
The article analyzes the relevance of research and developments in the field of Natural Language Processing (NLP), provides forecasts of the analytical communities, reviews the main methods and approaches of sentiment analysis of Russian-language texts. A separate block is devoted to the problems of researching the tonality of both Russian-language texts and the global problems faced by scholars around the world, regardless of language.
University EFL Instructors’ Attitude towards English Oral Interaction: A Case Study at Wollega University, Ethiopia
Authors: Nikussa Mekonnen Tolera 
Published: December 2023

The purpose of the current study was to investigate EFL instructors’ attitude towards English oral interaction in EFL classroom. For the study, a constructivism paradigm, a descriptive case study design and a qualitative research approach were employed. The participants were eight EFL instructors selected through purposive sampling technique. The data collection instrument was semi-structured interview. A phenomenological data analysis strategy was utilised. The data were analyzed in terms of the three components of attitude: cognitive, affective and behavioural components. Regarding cognitive component, all the EFL instructors perceived oral interaction as essential in promoting learners’ skills. Pertaining to affective component, the EFL instructors preferred oral interaction. Concerning behavioural component of their attitude towards oral interaction, the EFL instructors were enthusiastic to employ oral interaction in EFL classrooms. Therefore, the findings of the study portrayed that the EFL instructors had positive attitude towards oral interaction in the classroom. The findings indicated that, the EFL instructors utilized most of the oral interaction patterns in EFL classroom. Moreover, the EFL instructors employed the different patterns of English oral interaction most often in EFL classrooms.
PROPOSTA DE ADAPTAÇÃO DE UM PROGRAMA DE EDUCAÇÃO PARA O LAZER À REALIDADE BRASILEIRA
Authors: Carneiro Luciana Pereira de Moura , Ortuzar Aurora Madariaga , Zapirain Maria Begoña Garcia , Uvinha Ricardo Ricci 
Published: December 2023

Este estudo reflete sobre adaptações de um Programa de Educação para o Lazer (PEL) a ser aplicado com idosos brasileiros. Utilizando alguns termos de busca, identificamos, em quatro bases de dados, seis estudos que replicaram o PEL escolhido, além do próprio Searle, Mahon e Iso-Ahola (1995), que aplicaram o modelo pela primeira vez. Este PEL teve como base o “Community Reintegration Program” (CRP), desenvolvido por Bullock e Howe (1991) para pessoas com deficiência. Os países nos quais o PEL foi replicado foram Canadá e Taiwan. As adaptações são operacionais e de conteúdo. Assim como ocorreu quando adaptado à Taiwan, propomos que seja adaptado em temas como formato, facilitadores, equipe, frequência, atividades e local de oferta. Além disso, atividades lúdicas devem ser priorizadas na transmissão de seu conteúdo. A aplicação deste PEL pode contribuir para o debate do lazer como uma das mais importantes vias para o Envelhecimento Saudável no Brasil.
PROEKOLOŠKO PONAŠANJE KAO ISHOD EKOLOŠKE PISMENOSTI
Authors: Kalmar Laura , Marić Jurišin Stanislava 
Published: December 2023

Aktuelni ekološki problemi danas se mogu pripisati rastućoj populaciji, ekonomskom razvoju i industrijalizaciji, zagađenju, urbanizaciji i iscrpljivanju resursa na globalnom nivou. U osnovi, ovi tekući problemi uglavnom su povezani sa životnim stilovima ljudi i njihovim intenzivnim aktivnostima koje se odvijaju u prirodnom okruženju (Barr & Gilg 2006; Kolmuss & Agyman, 2002), što apostrofira značaj balansiranja odnosa između čoveka i prirodnog okruženja. Kao posledica toga poslednjih godina pitanja životne sredine su postala primarna u cilju prevazilaženja postojećih ekoloških problema. Ekološka pismenost je proglašena za jedan od najvažnijih aspekata ekološkog obrazovanja koje treba uzeti u obzir za upravljanje ekološkim problemima od 1970-ih. Ekološka pismenost predstavlja cilj ekološkog obrazovanja, a proekološko ponašanje tretira se kao ishod ekološke pismenosti. Važnost potrebe obrazovanja u rešavanju ekoloških problema i razvijanju ekološke pismenosti budućih generacija naglašena je na brojnim međunarodnim konferencijama, koje su poznate kao prekretnice u ekološkoj agendi. U radu je predstavljen pojam ekološkog obrazovanja, kao i pojam ekološke pismenosti. Nadalje, dat je pregled komponenti ekološke pismenosti. Ovaj rad ima za cilj da ukaže na pojam ekološkog obrazovanja, suštinu ekološke pismenosti, kao i na pojam i cilj proekološkog ponašanja. U domaćoj literaturi se nedovoljno piše o pojmu i značaju ekološke pismenosti, te ovim radom nastojimo da popunimo tu prazninu.
On the use of 3D camera to accurately measure volume and weight of dairy cow feed
Authors: Cotticelli Alessio , Verde Maria Teresa , Liccardo Annalisa , De Alteriis Giorgio , Lamonaca Francesco , Matera Roberta , Neglia Gianluca , Peric Tanja , Prandi Alberto , Bonavolontà Francesco 
Published: December 2023

The paper discusses the challenges facing the dairy industry due to increased farm sizes and reduced staff-to-animal ratios, which are impacting animal welfare. The development of precision livestock farming (PLF) technologies has gained momentum to address these challenges. PLF technologies can assess animal welfare and health status by monitoring animal behavior and biological changes, and alerting farmers of any issues. However, the applicability of PLF tools in other productive phases of the dairy cattle is still limited. The article focuses on the challenges of managing unweaned dairy calves, particularly the variability in relation to when calves start consuming solid feed, and how PLF technologies can be used to monitor individual calf intake and manage weaning at the individual level. The attention is mainly focused on the advantages of using automated feeders for unweaned dairy calves, including labor savings, greater precision in measurement and control of individual intake of liquid and solid feed, and higher preweaning growth rates. In particular, a method is proposed, involving a 3D depth camera and a proper algorithm to measure the volume and weight of eaten feed. The method is preliminarily assessed in tests conducted in laboratory, which highlight a remarkable concurrence (differences as low as 2 %) with respect to nominal values.
Acute myeloneuropathy due to Glutaric aciduria-1: expanding the phenotypic spectrum.
Authors: Baskar Dipti , Christopher Rita , Arunachal Gautham , Anud Davuluri Durga Srinivas , Mounika Ambati , Sangeeth TA , Polavarapu Kiran , Shalini BS , Joshi Tarachand , Sanka Sai Bhargava , Nashi Saraswati , Raja Pritam , Chowdary Ravindranadh Mundlamuri , Yadav Ravi , Nalini Atchayaram , Vengalil Seena 
Published: December 2023

Abstract
Background: Glutaric aciduria type-1 (GA-1) is a rare metabolic disorder due to mutation in GCDH gene resulting in varied clinical manifestations. Here we report a case of adult-onset GA-1 with acute myelo-neuropathy and chronic renal failure. Institutional ethics committee approval was obtained and genetic analysis was done by clinical exome sequencing.
Case presentation: A 19 year-old-man with 2 years history of chronic renal disease presented with 5 months history of sudden onset weakness of proximal and distal lower limbs and bladder retention. This was preceded by recurrent episodes of vomiting. On examination he had features of myeloneuropathy. Evaluation showed significant elevation of blood glutaryl carnitine with very low free carnitine along with brain MRI showing extensive white matter signal changes with diffusion restriction, subependymal nodules and involvement of internal capsule. Diagnosis was confirmed by clinical exome sequencing which showed a novel pathogenic homozygous missense mutation in exon 11 of GCDH gene (c.120C>T, p.His403Tyr).
Conclusions: This report expands phenotypic spectrum of GA-1 to include late onset acute myelo-neuropathy with chronic renal failure. A high index of suspicion is required since early treatment might decelerate further disease progression.
The impact of gestational diabetes mellitus on the development of pregnancy outcomes in twin pregnancies: a retrospective cohort study conducted at a tertiary hospital
Authors: Wu Hanglin , Zhang Lin , Xie Zhen , Cai Hongxia , Zhang Jindi , Yu Limin 
Published: December 2023

Abstract

Background: The impact of gestational diabetes mellitus (GDM) on pregnancy outcomes in twin pregnancies has not been fully understood. This study aimed to examine the effects of GDM on the risk of pregnancy outcomes in twin pregnancies and to investigate the relationship between glycemic levels and the risk of preeclampsia (PE) and abnormal fetal growth.
Methods: A retrospective cohort study of 736 twin pregnancies was conducted at a tertiary hospital in Hangzhou, China. Propensity score matching and multivariable logistic models were utilized to compare maternal and neonatal outcomes between twin pregnancies with GDM and those without GDM. Multivariable logistic regressions were performed to address the intertwin correlation between glycemic levels and the primary outcomes. Results were reported as odds ratios (ORs) with 95% confidence intervals (CIs).
Results: There was no significant difference in the risk of PE between non-GDM and GDM pregnancies (OR, 0.70; 95% CI: 0.38-1.27; P = 0.238). Women without GDM were less likely to require admission to the intensive care unit compared with women with GDM (OR, 0.19; 95% CI: 0.06-0.57; P = 0.003). No significant differences were found in the other maternal and neonatal outcomes between the study groups, including small and large for gestational age. Women with fasting blood glucose levels from 5.1 mmol/L to less than 5.3 mmol/L had a significantly higher risk of PE compared with women without GDM (OR, 2.90; 95% CI: 1.12-7.51; P = 0.028). In subgroups of HbA1c, HbA1c ≥ 5.5% had the highest risk of PE in the second and third trimesters compared with women without GDM (OR, 4.90; 95% CI: 1.00-24.12; P = 0.05).
Conclusion: The risk of PE was not increased in twin pregnancies complicated with GDM, but significantly increased in women with an HbA1c ≥5.5%. Twin pregnancies with GDM had a higher risk of admission to the intensive care unit compared with non-GDM twin pregnancies. Strict glycemic control may decrease the risk of PE in twin pregnancies with GDM.
Comparative analysis on genome-wide of NF-Y transcription factors between ‘Pinot Noir’ and Vitis amurensis
Authors: Lu Shixiong , Zhou Xueting , Huang Xu , Zeng Baozheng , Gou Huiming , Ma Weifeng , Ma Zonghuan , Mao Juan , Chen Bai-Hong 
Published: December 2023

Abstract
Nuclear factor Y (NF-Y) play a crucial role in the response of plants to abiotic stresses. However, there is a lack of research on the comparative analysis of bioinformatics and RT-qPCR of NF-Y transcription factors in ‘Pinot Noir’ and Vitis amurensis. In this study, a total of 27 and 26 NF-Y transcription factors were identified in ‘Pinot Noir’ and Vitis amurensis, respectively，and were divided into 3 subgroups. The subcellular localization prediction revealed that they were mainly located in the nucleus. Conserved motif analysis showed that five motifs from the NF-YB transcription factors exhibited higher conservation than the NF-YA and NF-YC transcription factors. The analysis of NF-Y transcription factor promoters showed that there were various stress-related elements such as drought, anaerobic induction, and ABA, etc. RT-qPCR analysis of ‘Pinot Noir’ and ‘Zuoyouhong’ plantlets demonstrated that the expression levels of VaNF-YA6, VaNF-YB5, VvNF-YA3, VvNF-YA5, and VvNF-YC2, etc. significantly upregulated under 400 mmol·L-1 NaCl and 10% PEG treatments. Furthermore, subcellular localization using tobacco leaves analysis showed that the VaNF-YA6-GFP fusion protein was primarily located in the nucleus. The overexpression of VaNF-YA6 in Arabidopsis can significantly enhance Arabidopsis tolerance to salt and drought stresses by activating antioxidant enzyme activity.
Identifying differences in molecular characteristics relevant for remodelling of periodontal ligament stem cells from upper and lower jaw
Authors: Malyaran Hanna , Craveiro Rogerio B. , Mert Sinan , Niederau Christian , Maas Sanne Lidewij , Vorst Emiel Petrus Carla van der , Hölzle Frank , Jahnen-Dechent Wilhelm , Wolf Michael , Neuss Sabine 
Published: December 2023

Abstract
Background
Clinical experience holds that the precise localization of periodontal defects greatly influences the velocity and effectiveness of wound healing and bone remodelling. Periodontal healing is quicker and more efficient in the maxilla (upper jaw) than in the mandible (lower jaw). Differences in blood supply, innervation, and odontogenesis of upper vs. lower jaw may all influence healing, but cell-intrinsic differences may also exist. Few studies focused on cell signaling in mechanically stimulated human periodontal ligament stem cells (PDLSC), none of which considered the differences between mandible and maxilla.
Methods
To unravel differences in molecular mechanisms and pathways in PDLSC using kinomics technology. Cells were characterized in terms of stem cell surface markers (CD34, CD45, CD73, CD90 and CD105), proliferation and differentiation capacity (adipogenic, osteogenic and chondrogenic). Besides, the kinase activity was analyzed via a multiplex kinase activity profiling technology. The phosphorylation status of cellular proteins in upper and lower jaw PDLSC cells from healthy donors is presented. We map differential kinase activity in known gene regulatory networks using network analysis to explain the observed cell-intrinsic differences in growth and regeneration of these cells.
Results
Upstream kinase analysis revealed two EphA receptors that were significantly stronger expressed in the mandible compared to maxilla. EphA4 signaling is known to inhibit osteogenic differentiation. Pathway analysis revealed that PI3K-Akt pathway is more active in the lower jaw.
Conclusion
Our findings showed that PDLSC from upper jaw have a significant higher proliferation rate and better differentiation capability. Results indicate differential activation of gene regulatory pathways in PDLSC from upper vs. lower jaw, which should be considered in studies regarding the regenerative capacity of PDLSC.
Excessive erythrophagocytosis accounts for systemic inflammation in chronic kidney disease
Authors: Meng Qian , Yang Xiaowei , You Guoxing , Chen Wanyi , Zhao Bing , Zhu Huizi , Xu Liang , Zhou Yan , Liu Xiang , Zhai Chunjuan , Wang Rong , Sun Jing , Zhao Lian 
Published: December 2023

Abstract
Objective and design
Chronic kidney disease (CKD) is associated with persistent systemic inflammation. Reduced red blood cell (RBC) survival in patients with CKD has been identified for several decades. The purpose of this study is to explore whether excessive erythrophagocytosis exists and contributes to systemic inflammation in CKD.
Methods and subjects
A CKD rat model was induced by 5/6 nephrectomy. Erythrophagocytosis was evaluated in vivo and in vitro. RT-qPCR and immunohistochemistry were used to determine the inflammatory effects after erythrophagocytosis.
Results
Erythrocyte osmotic fragility and deformability progressively declined, and the percentage of PS-exposing RBCs progressively increased in CKD rats. More of erythrocytes from 5/6Nx rats were phagocytosed by macrophages in comparison to those from control rats in vivo and in vitro. Macrophages phagocytosed CKD erythrocytes exhibited higher mRNA levels of IL-6, CXCL-10, CXCL-11, iNOS, IL-1β, ICAM-1 and MCP-1. The red pulp of rats with CKD exhibited higher levels of p-NFκB, IL-6, iNOS and CXCL-10. ELISA results showed significantly increased plasma levels of both IL-6 and CXCL-10 in patients with long-term hemodialysis compared with those in healthy controls.
Conclusions
Our results indicated that excessive erythrophagocytosis might be an important source of systemic inflammation in CKD.
Spatial Data Mining for Prediction of Unobserved Zinc Pollutant using Various Kriging Methods
Authors: Krotha Durga pujitha , SK Fathimabi , G JayaLakshmi , M Suneetha 
Published: December 2023

Abstract
After years of contamination, rivers may get large amounts of heavy metal pollution. Our investigation's goal is to identify the river's hazardous locations. In our study case, we select the zinc-contaminated floodplains of the Meuse River (Zn). Excessive zinc levels may lead to a variety of health issues, including anemia, rashes, vomiting, and cramping in the stomach. However, there isn't a lot of sample data available about the Meuse River's zinc concentration; as a result, it's necessary to generate the missing data in unidentified regions. This study employs universal Kriging in spatial data mining to explore and predict unknown zinc pollutants. The semivariogram is a useful tool for representing the variability pattern of zinc. To predict the unknown regions, this captured model will be interpolated using the Kriging method. Regression with geographic weighting makes it possible to see how stimulus-response relationships change over space. We use a variety of semivariograms in our work, such as matern, exponential, and linear models. We also propose Universal Kriging and geographically weighted regression. The experimental findings show that: (i) the matern model, as determined by calculating the minimum error sum of squares, is the best theoretical semivariogram model; and (ii) the accuracy of the predictions can be visually demonstrated by projecting the results onto the real map.
The Heterogeneous Effects of the First Childbirth on Women’s Income
Authors: Jahromi Afrouz Azadikhah , Huang Weige 
Published: December 2023

Abstract

This study estimates the heterogeneous effects of the first childbirth on women's annual income in the United States using the National Longitudinal Survey of Youth data from 1979 to 2018. Women usually experience an immediate decrease in their income after childbirth relative to what they would have earned if they had not become mothers. The gap closes somewhat over time though mothers never fully catch up to their counterfactuals. Previous work tried to explain the motherhood penalty by estimating the average treatment effect of children on women's income; however, these effects can be quite heterogeneous across mothers with different observable characteristics. In this study, our interest centers on the distribution of the individual-level effect of the first childbirth on mothers' income using the Changes-in-Changes model and quantile regression. Identifying the features of this distribution is a challenging task as it requires the knowledge of joint distribution. We find that around 73% of mothers have lower income after their first childbirth relative to what they would have had if they had not had a child.. Moreover, the adverse effects of motherhood on income are particularly pronounced among 10-20% of mothers. Our quantile regression analysis, documented in the Appendix, indicates that the first childbirth most negatively affects older, single/divorced, white, more educated mothers, and those with higher potential earnings had they not given birth.