bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of the carrot miRNAome in response to salt stress
Authors: Szymonik Kamil , Klimek-Chodacka Magdalena , Lukasiewicz Aneta , Macko-Podgórni Alicja , Grzebelus Dariusz , Baranski Rafal 
Published: December 2023

AbstractSoil salinity adversely affects the yield and quality of crops, including carrot. During salt stress, plant growth and development are impaired by restricted water uptake and ion cytotoxicity, leading to nutrient imbalance and oxidative burst. However, the molecular mechanisms of the carrot plant response to salt stress remain unclear. The occurrence and expression of miRNAs that are potentially involved in the regulation of carrot tolerance to salinity stress were investigated. The results of small RNA sequencing revealed that salt-sensitive (DH1) and salt-tolerant (DLBA) carrot varieties had different miRNA expression profiles. A total of 95 miRNAs were identified, including 71 novel miRNAs, of which 30 and 23 were unique to DH1 and DLBA, respectively. The comparison of NGS and qPCR results allowed identification of two conserved and five novel miRNA involved in carrot response to salt stress, and which differentiated the salt-tolerant and salt-sensitive varieties. Degradome analysis supported by in silico-based predictions and followed by expression analysis of exemplary target genes pointed at genes related to proline, glutathione, and glutamate metabolism pathways as potential miRNA targets involved in salt tolerance, and indicated that the regulation of osmoprotection and antioxidant protection, earlier identified as being more efficient in the tolerant variety, may be controlled by miRNAs. Furthermore, potential miRNA target genes involved in chloroplast protection, signal transduction and the synthesis and modification of cell wall components were indicated in plants growing in saline soil.
Artemisia carvifolia Buch silver nanoparticles downregulate the Rap2A gene in liver cancer
Authors: Javid Sabahat , Dilshad Erum 
Published: December 2023

AbstractLiver cancer is the second main reason of death globally. In the current study, Rap2A protein a member of Ras Gtpase was selected as a drug target for liver cancer which has been identified as an oncogene in different types of tumors. The present study aimed to evaluate Artemisia carvifolia Buch extract and its silver nanoparticles against liver cancer targeting the Rap2A gene. The synthesized silver nanoparticles showed an absorbance peak at 450 nm by a UV–Vis spectrophotometer. SEM revealed that polyhedral silver nanoparticles had a size ranging from 80 ± 6 nm. Furthermore, amines, aldehydes, ketones and alcohols of Artemisia carvifolia were found involved in the reduction and stabilization of nanoparticles by FTIR. Moreover, XRD and EDX confirmed the cubic crystalline nature and particle elemental composition, respectively. Furthermore, the cytotoxicity against HePG2 cancer cell lines was also found significant with an IC50 value of 2.57 µM for silver nanoparticles and 11.57 µM for plant extract. The gene expression and protein level of Rap2A were also decreased in plant extract and nanoparticle-treated cells compared to control groups. The apoptotic potential of extract and nanoparticles was also determined by evaluating the apoptotic pathway genes and protein including BAX, caspase 3, 8 and 9. Significantly elevated levels of expression of these genes by real-time qPCR along with increased protein levels by ELISA were found. This is the first-ever report describing the synthesis and efficacy of silver nanoparticles of Artemisia carvifolia Buch against liver cancer.
Early brain microstructural development among preterm infants requiring caesarean section versus those delivered vaginally
Authors: Bobba Pratheek S. , Weber Clara F. , Malhotra Ajay , Bahtiyar Mert O. , Copel Joshua , Taylor Sarah N. , Ment Laura R. , Payabvash Seyedmehdi 
Published: December 2023

AbstractIt is known that the rate of caesarean section (C-section) has been increasing among preterm births. However, the relationship between C-section and long-term neurological outcomes is unclear. In this study, we utilized diffusion tensor imaging (DTI) to characterize the association of delivery method with brain white matter (WM) microstructural integrity in preterm infants. We retrospectively analyzed the DTI scans and health records of preterm infants without neuroimaging abnormality on pre-discharge term-equivalent MRI. We applied both voxel-wise and tract-based analyses to evaluate the association between delivery method and DTI metrics across WM tracts while controlling for numerous covariates. We included 68 preterm infants in this study (23 delivered vaginally, 45 delivered via C-section). Voxel-wise and tract-based analyses revealed significantly lower fractional anisotropy values and significantly higher diffusivity values across major WM tracts in preterm infants delivered via C-section when compared to those delivered vaginally. These results may be partially, but not entirely, mediated by lower birth weight among infants delivered by C-section. Nevertheless, these infants may be at risk for delayed neurodevelopment and could benefit from close neurological follow up for early intervention and mitigation of adverse long-term outcomes.
Bland–Altman agreement analysis between CT predicted and surgical peritoneal cancer index in pseudomyxoma peritonei of appendiceal origin
Authors: Bai Mingjian , Chen Jingliang , Xu Yueming , Feng Jing , Ma Ruiqing , Jia Hongmin , Xu Hongbin , Liang Guowei , Wei Hongjiang 
Published: December 2023

AbstractPeritoneal cancer index (PCI) is the surgical variable most commonly used to quantify the extent of peritoneal metastases for pseudomyxoma peritonei (PMP) patients. The present study aimed to investigate the agreement between CT predicted and surgical PCI by the Bland–Altman method for PMP of appendiceal origin. A total of 167 PMP patients of appendiceal origin were included between 2016 and 2021. Bland–Altman analysis was performed for both total PCI and selected PCI (regions 2 + 9–12). After the Bland–Altman plot was drawn, the mean bias and its 95% limit of agreements (LoAs) was quantified. Besides, the correlation coefficients between CT-PCI and surgical PCI were also been calculated. The Bland–Altman plot showed the mean bias ± SD between total CT-PCI and surgical PCI as 0.431 ± 3.005, with the LoAs from − 5.459 to 6.321. There were nine points of difference in total PCI exceeded the 95% LoAs, with the rate of 5.39% (9/167). As for selected CT-PCI, Bland–Altman plot showed the mean bias ± SD between selected CT-PCI and surgical PCI as − 0.287 ± 1.955, with the LoAs from − 4.118 to 3.544. There were ten points of difference in selected PCI exceeded the 95% LoAs, with the rate of 5.99% (10/167). The Spearman's rank correlation coefficient between total CT-PCI and surgical PCI was 0.911, P < 0.001, as for selected CT-PCI and surgical PCI, the coefficient was 0.909, P < 0.001. Although there was a strong correlation for both total and selected CT-PCI with surgical PCI, however, the agreement is still not good in Bland–Altman analysis, which suggested that CT-PCI cannot predict surgical PCI accurately even in professional PMP treatment centers. In brief explanation, CT makes it difficult to distinguish the borderline between tumor tissue and mucus and to detect tumor lesions in the small intestine regions, which caused overestimation or underestimation by CT-PCI. In the future, a multiple linear regression model based on CT-PCI might accurately predict surgical PCI preoperatively.
A chromosome-level genome assembly for the Silkie chicken resolves complete sequences for key chicken metabolic, reproductive, and immunity genes
Authors: Zhu Feng , Yin Zhong-Tao , Zhao Qiang-Sen , Sun Yun-Xiao , Jie Yu-Chen , Smith Jacqueline , Yang Yu-Ze , Burt David W. , Hincke Maxwell , Zhang Zi-Ding , Yuan Meng-Di , Kaufman Jim , Sun Cong-Jiao , Li Jun-Ying , Shao Li-Wa , Yang Ning , Hou Zhuo-Cheng 
Published: December 2023

AbstractA set of high-quality pan-genomes would help identify important genes that are still hidden/incomplete in bird reference genomes. In an attempt to address these issues, we have assembled a de novo chromosome-level reference genome of the Silkie (Gallus gallus domesticus), which is an important avian model for unique traits, like fibromelanosis, with unclear genetic foundation. This Silkie genome includes the complete genomic sequences of well-known, but unresolved, evolutionarily, endocrinologically, and immunologically important genes, including leptin, ovocleidin-17, and tumor-necrosis factor-α. The gap-less and manually annotated MHC (major histocompatibility complex) region possesses 38 recently identified genes, with differentially regulated genes recovered in response to pathogen challenges. We also provide whole-genome methylation and genetic variation maps, and resolve a complex genetic region that may contribute to fibromelanosis in these animals. Finally, we experimentally show leptin binding to the identified leptin receptor in chicken, confirming an active leptin ligand-receptor system. The Silkie genome assembly not only provides a rich data resource for avian genome studies, but also lays a foundation for further functional validation of resolved genes.
Design and application of a novel telemedicine system jointly driven by multinetwork integration and remote control: Practical experience from PLAGH, China
Authors: Wang Ruiqing , Zhang Jie , He Shilin , Guo Huayuan , Li Tao , Zhong Qin , Ma Jun , Xu Jie , He Kunlun 
Published: December 2023

AbstractIn China, several problems were common in the telemedicine systems, such as the poor network stability and difficult interconnection. A new telemedicine system jointly driven by multinetwork integration and remote control has been designed to address these problems. A multilink aggregation algorithm and an overlay network for telemedicine system (ONTMS) were developed to improve network stability, and a non‐intervention remote control method was designed for Internet of Things (IoT) devices/systems. The authors monitored the network parameters, and distributed the questionnaire to participants, for evaluating the telemedicine system and services. Under a detection bandwidth of 8 Mbps, the aggregation parameters of Unicom 4G, Telecom 4G, and China Mobile 4G were optimal, with an uplink bandwidth, delay, and packet loss ratio (PLR) of 7.93 Mbps, 58.80 ms, and 0.06%, respectively. These parameters were significantly superior to those of China Mobile 4G, the best single network (p < 0.001). Through the ONTMS, the mean round‐trip transporting delay from Beijing to Sanya was 76 ms, and the PLR was 0 at vast majority of time. A total of 1988 participants, including 1920 patients and 68 doctors, completed the questionnaires. More than 97% of participants felt that the audio and video transmission and remote control were fluent and convenient. 96% of patients rated the telemedicine services with scores of 4 or 5. This system has shown robust network property and excellent interaction ability, and satisfied the needs of patients and doctors.
Investigating the long secondary period phenomenon with the ASAS-SN and Gaia data
Authors: Pawlak Michał , Trabucchi Michele , Eyer Laurent , Mowlavi Nami 
Published: December 2023

Aims. The aim of this work is to create a complete list of sources exhibiting a long secondary period (LSP) in the ASAS-SN catalog of variable stars, and analyze the properties of this sample compared to other long period variables without an LSP.
Methods. We used the period-amplitude diagram to identify the 55 572 stars showing an LSP, corresponding to 27% of the pulsating red giants in the catalog. We used astrometric data from Gaia DR3 and spectroscopic data provided by the APOGEE, GALAH, and RAVE surveys to investigate the statistical properties of the sample.
Results. We find that stars displaying an LSP have a spatial distribution that is more dispersed than that of the non-LSP giants, suggesting that they belong to an older population. Spectroscopically derived ages seem to confirm this. The stars with an LSP also appear to be different in terms of the C/O ratio from their non-LSP counterparts.
Cosmological constraints from the abundance, weak lensing, and clustering of galaxy clusters: Application to the SDSS
Authors: Fumagalli A. , Costanzi M. , Saro A. , Castro T. , Borgani S. 
Published: December 2023

Aims. The clustering of galaxy clusters is a powerful cosmological tool. When it is combined with other cosmological observables, it can help to resolve parameter degeneracies and improve constraints, especially on Ωm and σ8. We aim to demonstrate its potential in constraining cosmological parameters and scaling relations when combined with cluster counts and weak-lensing mass information. As a case study, we use the redMaPPer cluster catalog derived from the Sloan Digital Sky Survey (SDSS).
Methods. We extended a previous analysis of the number counts and weak-lensing signal by the two-point correlation function. We derived cosmological and scaling relation posteriors for all possible combinations of the three observables to assess their constraining power, parameter degeneracies, and possible internal tensions.
Results. We find no evidence for tensions between the three data sets we analyzed. We demonstrate that the constraining power of the sample can be greatly improved by including the clustering statistics because this can break the Ωm − σ8 degeneracy that is characteristic of cluster abundance studies. In particular, for a flat ΛCDM model with massive neutrinos, we obtain Ωm = 0.28 ± 0.03 and σ8 = 0.82 ± 0.05, which is an improvement of 33% and 50% compared to the posteriors derived by combining cluster abundance and weak-lensing analyses. Our results are consistent with cosmological posteriors from other cluster surveys, and also with Planck results for the cosmic microwave background (CMB) and DES-Y3 galaxy clustering and weak-lensing analysis.
Vitioeno, un centre de ressources numériques pour la production de données scientifiques FAIR
Authors: Charleroy Arnaud , Delpuech Xavier , Neveu Pascal 
Published: December 2023

Résumé
INRAE et l’IFV ont mis en place un centre de ressources numériques ouvertes et librement accessibles, avec l’objectif d’aider et de faciliter la structuration et la qualification des données selon les principes FAIR. Ce centre de ressource pour l’aide à la gestion des données « Vitioeno » est disponible en ligne, en français et en anglais, à l’adresse https://vitioeno.mistea.inrae.fr/resource/app/. Il recense et donne un accès ouvert à différents types de ressources numériques existantes, en particulier des référentiels (matériel végétal et variables expérimentales), des documents téléchargeables de type protocoles, et des applications ou liens utiles.
Estudio de precursores aromáticos en clones comerciales de Tannat y seguimiento de su evolución durante la maduración
Authors: Nieto N. , Da Silva C. , Coniberti A. , Boido E. , Carrau F. , Dellacassa E. , Fariña L. 
Published: December 2023

Resumen
La variedad Tannat desde el punto de vista del aroma, resulta una variedad neutra pero capaz de aportar precursores aromáticos que son de interés en la elaboración de vinos de guarda. Los principales precursores aromáticos presentes son compuestos glicosidados y carotenoides. Durante 3 vendimias consecutivas se estudió el contenido de precursores aromáticos de 8 clones comerciales de Tannat (ENTAV-INRA: 398, 399, 472, 474, 475, 717, 794 Y 944). En base a los resultados obtenidos se seleccionaron los clones 717 y 474 por su alta y baja performance en la producción de precursores glicosidados. En la siguiente vendimia se estudió el comportamiento de precursores glicosidados (SPE-GC-MS) y carotenoides (HPLC-DAD) en los clones seleccionados durante 4 estadíos de madurez de la uva, donde se identificaron 14 xantofilas y 1 caroteno. Se encontraron diferencias significativas en el contenido de carotenoides entre los dos clones estudiados a lo largo del período de maduración. Mientras que su degradación no presentó diferencias significativas. Conjuntamente se identificaron 10 norisoprenoides glicosidados en la uva de los clones estudiados pero el contenido de estos no presentó diferencias significativas entre los clones durante está vendimia. Las diferencias entre los clones 717 y 474 son tales que no se expresan todos los años teniendo variabilidad con el año y donde el clima juega un rol fundamental.
Análisis metabolómica del zumo de uva brasileño mediante resonancia magnética nuclear de hidrógeno
Authors: Paula Drehmer Ana , Rodrigues Spinelli Fernanda , Moura Sidnei 
Published: December 2023

Resumen
La determinación de las propiedades químicas del zumo de uva es una forma de promover la difusión y valorización de este producto, considerando su importancia socioeconómica para los países que lo producen, siendo buscado como fuente de alimentación natural a nivel mundial. En este sentido, la Resonancia Magnética Nuclear (RMN) permite la identificación de compuestos, generando un perfil metabólico, y se ha aplicado para probar autenticidad, origen geográfico y añada. En ese contexto, este estudio buscó identificar patrones de similaridad entre zumos de uva producidos en el estado de Rio Grande do Sul/Brasil, utilizando 1H RMN y quimiometría. Se analizaron 42 muestras, preparadas diluyendo 20 μL de muestra en 580 μL de agua deuterada (D2O) y, posteriormente, se realizó el análisis 1H RMN. A partir de los espectros obtenidos se identificaron ácidos málico, tartárico y cítrico, alanina, además de α y β glucosa y fructosa. Además, la intensidad de las señales referentes al etanol y al ácido acético podría estar relacionada con la fermentación de las muestras. Por lo tanto, se aplicó el análisis quimiométrico Análisis por Componentes Principales (ACP) para ayudar a crear un estándar para separar y clasificar las muestras por región y cosecha. En resumen, esta es la primera investigación que informa patrones de metabolitos de zumos de uva de la región de la Serra Gaúcha, que es la mayor productora de la viña y el vino de Brasil. Esto puede aplicarse para la denominación de origen y/o identificación de fraudes en este producto.
État des lieux de l’enseignement de l’éducation thérapeutique du patient dans la formation initiale des sages-femmes françaises
Authors: Ohayon Emilie , Marchand Claire , Naudin David , Riquet Sébastien 
Published: December 2023

Objectifs : Cette étude vise à établir un état des lieux de l’enseignement de l’Éducation Thérapeutique du Patient (ETP) en formation initiale des sages-femmes françaises. Méthode : Une étude quantitative descriptive a été menée sur le territoire français. Un questionnaire en ligne, comportant 27 questions a été diffusé aux 35 écoles de sages-femmes françaises. Résultats : Sur 19 écoles ayant répondu à l’enquête, 11 enseignaient l’ETP, 8 ne l’abordaient pas en formation. Cet enseignement est principalement transversal. Les freins à l’enseignement de l’ETP sont la densité actuelle du programme, l’absence de texte réglementant cet enseignement et des difficultés à circonscrire le champ de l’ETP par rapport à celui de la prévention, de la promotion et de l’éducation pour la santé. La simulation est utilisée dans une seule école. Discussion : Cette enquête montre une volonté des équipes pédagogiques à investir dans l’enseignement des éducations en santé, notamment de l’ETP. Pour cela, il s’agit de renforcer la formation des enseignants afin de clarifier les domaines d’intervention de la sage-femme faisant appel à la promotion de la santé, la prévention et les éducations en santé ; de proposer des stages spécifiques aux étudiants et de faire appel à la simulation. L’allongement de la durée de la formation initiale constitue une opportunité de planifier un enseignement spécifique et de discuter la place du service sanitaire.
From Nasolabial Folds to Pan-facial Rejuvenation—The Evolution of Fillers in my Career
Authors: Grunebaum Lisa D. , Eitan Dana N. , Cave Taylor B. 
Published: December 2023

AbstractThis is a comprehensive review of facial fillers including landmark studies and expert commentary spanning the years from 2003 (when the first hyaluronic acid [HA] dermal filler underwent Food and Drug Administration approval in United States) to present.
Severe Unilateral Microtia with Aural Atresia, Hair White Patch, Stereotypes in a Young Boy with De novo 16p13.11 Deletion: Reasons for a New Genotype–Phenotype Correlation
Authors: Pavone Piero , Pappalardo Xena Giada , Parano Claudia , Parano Enrico , Corsello Antonio , Ruggieri Martino , Cacciaguerra Giovanni , Falsaperla Raffaele 
Published: December 2023

Abstract
Background Microtia is an uncommon congenital malformation ranging from mild anatomic structural abnormalities to partial or complete absence of the ear leading to hearing impairment. Congenital microtia may present as a single malformation (isolated microtia) or sometimes associated with other congenital anomalies involving various organs. Microtia has been classified in three degrees according to the complexity of the auricular malformation and to anotia referred to the total absence of the ear. Genetic role in causing auricular malformation has been widely demonstrated, and genotype–phenotype correlation has been reported in cases of syndromic microtia.
Case Presentation We report here a young patient with a third degree of scale classification and aural atresia. The patient showed unspecific facial dysmorphism, speech delay, precocious teething, hair white patch, and stereotypic anomalous movements. Genetic analysis displayed a de novo 16p13.11 deletion.
Conclusion Microtia with aural atresia is an uncommon and severe birth defect, which affects functional and esthetic aspects, often associated with other malformations. As traumatic this disorder may be for the parents, the microtia and aural atresia are treatable, thanks to the improving and evolving surgical techniques. Based on the genetic analysis and the clinical features observed in the present case, a genotype–phenotype correlation has been proposed.
Intervocalic /ɡ/ realization in Border Uruguayan Spanish
Authors: Gradoville Michael , Waltermire Mark , Chery Audrey , Fernandez Sofía , Long Avizia 
Published: December 2023

Abstract
In Border Uruguayan Spanish, intervocalic voiced obstruents have been known to be produced as stops due to the variety’s contact with Portuguese. The present study investigates intervocalic /ɡ/ in a corpus of sociolinguistic interviews. Using an acoustic measure, a consonant-vowel intensity ratio, as an index of constriction of /ɡ/, we found that, similar to /b/ and /d/, the speaker’s age, Spanish use, and sex have a strong impact on the realization of intervocalic /ɡ/. Specifically, younger speakers, those that speak Spanish most of the time, and men are likely to use less constricted variants such as approximants and elision. Given the parallels that these results have with findings of studies of intervocalic /d/ and, to a lesser extent, /b/ in this variety, we discuss support for the notion that the three phonemes behave as a series and not independently.