bluuebunny/crossref_metadata_2025_split huggingface dataset:
c-di-GMP inhibits rRNA methylation and impairs ribosome assembly in the presence of kanamycin
Authors: Yu Siqi , Hu Zheyao , Xu Xiaoting , Liang Xiaoran , Shen Jiayi , Liu Min , Lin Mingxi , Chen Hong , Marti Jordi , Tao Sheng-ce , Xu Zhaowei 
Published: January 2025

Abstract
Cyclic diguanosine monophosphate (c-di-GMP) is a ubiquitous bacterial secondary messenger with diverse functions. A previous Escherichia coli proteome microarray identified that c-di-GMP binds to the 23S rRNA methyltransferases RlmI and RlmE. Here we show that c-di-GMP inhibits RlmI activity in rRNA methylation assays, and that it modulates ribosome assembly in the presence of kanamycin. Molecular dynamics simulation and mutagenesis studies reveal that c-di-GMP binds to RlmI at residues R64, R103, G114, and K201. Structural simulations indicate that c-di-GMP quenches RlmI activity by inducing the closure of the catalytic pocket. We also show that c-di-GMP promotes antibiotic tolerance through RlmI. Binding and methylation assays indicate that the inhibitory effect of c-di-GMP on RlmI is conserved across various pathogenic bacteria. Our data suggest an unexpected role for c-di-GMP in regulating ribosome assembly under stress through the inhibition of rRNA methyltransferases.
Boosting human immunology: harnessing the potential of immune organoids
Authors: Moll Maximilian , Baumjohann Dirk 
Published: January 2025

Abstract
Studying the human immune system in vivo is challenging and often not possible. Therefore, most human immunology studies have been predominantly confined to peripheral blood analyses, which by themselves have inherent limitations, as many immune reactions take place within tissues. For example, potent antibody responses that contribute to fighting infections and provide protection following vaccination require cellular interactions between B cells and T cells in specialized micro-anatomical structures called germinal centers, which are found in secondary lymphoid organs such as spleen, lymph nodes, and tonsils. Thus, there is a clear demand for novel enhanced experimental systems that faithfully recapitulate the intricate dynamics of the human immune system as much as possible. In this review, we discuss recent advances in versatile human tonsil/adenoid tissue-based ex vivo immune organoid cultures as well as related cancer and autoimmunity-focused experimental setups. These systems have been implemented as translational immunology platforms for in-depth analyses of human B and T cell-mediated immune responses, thereby facilitating mechanistic studies as well as drug and vaccine testing in a human-first approach.
ALMA observations of super-early galaxies: Attenuation-free model predictions
Authors: Ferrara A. , Carniani S. , di Mascia F. , Bouwens R. J. , Oesch P. , Schouws S. 
Published: January 2025

The abundance and blue color of the super-early (redshift z > 10) luminous galaxies discovered by the James Webb Space Telescope (JWST) can be explained if radiation-driven outflows have ejected their dust on kiloparsec-scales. To test this hypothesis, we predict the Atacama Large Millimeter Array (ALMA) detectability of this extended dust component. Based on the observed properties of the galaxy, its observed continuum flux at 88 μm, F88, depends on the dust-to-stellar mass ratio, ξd, and on the extent of the dust distribution, rd. When this is applied to the most distant known galaxy, GS-z14-0 at z = 14.32, the fiducial model (ξd = 1/529) predicts
F88fid = 14.9 μJy and a dust extent rd = 1.4 kpc. If the galaxy is very dust rich (ξd = 1/40),
F88max = 40.1 μJy. These values are lower
(F88fid = 9.5 μJy) if the dust is predominantly made of large grains such as those that form in supernova ejecta. Forthcoming ALMA observations might come very close to constraining the fiducial predictions of the outflow-based attenuation-free model. Other super-early galaxies are predicted to be fainter at 88 μm, mostly because their star formation rate is lower than that of GS-z14-0, with fiducial fluxes in the range 2 − 5.2 μJy.
First D0 + D0 measurement in nucleus-nucleus collisions at SPS energies with NA61/SHINE
Authors: Merzlaya Anastasia 
Published: January 2025

The measurement of open charm meson production provides a tool for the investigation of the properties of the hot and dense matter created in nucleus-nucleus collisions at relativistic energies. In particular, charm mesons are of vivid interest in the context of the study of the nature of the phasetransition between confined hadronic matter and the quark-gluon plasma. Recently, the NA61/SHINE experimental setup was upgraded with a high spatial resolution Vertex Detector which enables the reconstruction of secondary vertices from open charm meson decays.
The first direct neutral D meson yields at the SPS energy regime will be shown. The analysis used the 20% most central Xe+La collisions at 150A GeV/c from the data set collected in 2017. It allowed the estimation of the mid-rapidity yields (dN/dy) of D0 + D¯0 using their π± + K∓ decay channels and phase space corrections derived from the three models AMPT, PHSD, and PYTHIA/Angantyr. The results will be compared and discussed in the context of several model calculations including statistical and dynamical approaches.
Wie gut ist die Wirksamkeit der Low-Dose-Radiotherapie (LD-RT) für die Heberden-Arthrose? Eine Analyse der aktuellen Literatur
Authors: Schreiner Ute , Huberti Helmut 
Published: January 2025

ZusammenfassungDie Low-Dose-Radiotherapie ist eine etablierte Behandlungsoption nicht-maligner
Erkrankungen der Bewegungsorgane. In der Behandlung der Heberden-Arthrose wird sie
eingesetzt, bisher scheint eine Evidenz der Wirksamkeit jedoch nicht sicher. In dieser
Arbeit wird die aktuelle Literatur überprüft auf wissenschaftliche Nachweise einer
Wirksamkeit in der Behandlung der Heberden-Arthrose.Die Datenbanken PubMed und Cochrane Library wurden auf entsprechende Publikationen
durchsucht.Es konnten 9 Publikationen identifiziert werden, die Daten aus 7 Studien
veröffentlichen. Nur eine Studie war als randomisiert, placebokontrolliert und verblindet
konzipiert. Keine der Studien untersuchte ausschließlich Heberden-Arthrose. Die Studien
waren durchweg inhomogen in Hinblick auf Einschlusskriterien, Nachuntersuchungskriterien,
Bestrahlungsmodus und Interpretation des Behandlungserfolgs. In der RCT konnte kein
Unterschied zwischen der Verumgruppe und der Kontrollgruppe festgestellt werden.Die Studienlage ist derzeit schwach. Die recherchierten Publikationen sind zu wenig
zielgerichtet auf das Kollektiv der Heberden-Arthrose und insgesamt zu inhomogen in Hinblick
auf die angewendeten Kriterien. Von daher sind künftige zielgerichtete Studien für den
Nachweis einer Wirksamkeit erforderlich.
Changes in Coagulation Factor XI Activity Levels in Patients with ST-Segment Elevation Myocardial Infarction Undergoing Primary PCI
Authors: Spagnolo Marco , Ammirabile Nicola , Cutore Luigi , Di Leo Giacinto , Finocchiaro Simone , Giacoppo Daniele , Greco Antonio , Imbesi Antonino , Landolina Davide , Laudani Claudio , Mauro Maria Sara , Mazzone Placido Maria , Capodanno Davide 
Published: January 2025

Background Although factor XI (FXI) inhibitors are currently tested for the prevention of thrombotic events, their early treatment could prevent thrombus consolidation in ST-segment elevation myocardial infarction (STEMI). This study aims to characterize coagulation FXI levels and their variations in patients with STEMI undergoing primary percutaneous coronary intervention (PCI).
Methods Patients with STEMI were prospectively enrolled between December 2023 and May 2024. FXI activity (FXIa) levels were measured at admission and after PCI (i.e., before discharge). Variations in FXIa levels were evaluated. Differences in indicators of thrombotic risk between groups with high and low FXIa variability were analyzed, and predictors of high FXIa variability were identified.
Results After screening, 54 patients with STEMI were included. The median FXIa level was 0.865 IU/mL (interquartile range [IQR] 0.554–0.978) at admission and 1.161 IU/mL (IQR 0.982–1.317) before discharge, with a median difference of +34.2% (p-value < 0.001). No significant differences were found in indicators of thrombotic risk between groups at high and low FXIa variability, except for the days intercurred between the assays (p-value = 0.016). Neither this nor other variables emerged as independent predictors of high FXIa variability.
Conclusion This study first reported an increase in FXIa levels from admission to discharge in STEMI patients undergoing PCI. Common indicators of thrombotic risk were not associated with FXIa levels or their variability. These findings aim to stimulate further research into anticoagulant therapies tailored to the patient's coagulative state and disease.
Feasibility of Pedal Access for Geniculate Artery Embolization
Authors: Hasan Eusha , Saifuddin Ali , Schuchardt Philip , Khoury Joe , Al-Roubaie Mustafa 
Published: January 2025

AbstractGeniculate artery embolization is a treatment option for refractory knee osteoarthritis in patients unable to receive total knee arthroplasty. This case report describes two patients for whom pedal access was used to embolize the geniculate arteries due to challenging groin anatomy secondary to significant comorbidities (morbid obesity, proximal atherosclerosis). Both patients achieved marked pain relief and enhanced knee function at 1-month follow-up. These cases suggest that pedal access is a viable alternative, offering a promising approach for patients with unfavorable anatomy and comorbidities that make groin access impractical. Further research is needed to validate these findings in larger patient populations.
Measuring the effects of Crime Prevention Through Environmental Design (CPTED) on fear of crime in public spaces
Authors: Senna Isangelo , Iglesias Fabio , Matsunaga Lucas Heiki 
Published: January 2025

Abstract
Despite decades of research on Crime Prevention Through Environmental Design (CPTED), the specific impact of its dimensions on fear of crime (FoC) remains under-examined. This study investigates these effects by analyzing responses from 460 participants who evaluated photographs and completed the Situational Fear of Crime Scale for Public Spaces (SFS-Scale). The findings reveal that deficiencies in natural surveillance, followed by a lack of territoriality, are linked to heightened FoC. Moreover, natural surveillance emerged as a more significant predictor of FoC compared to territoriality. Additionally, police officers and military personnel reported lower levels of FoC than the general public. The study highlights the importance of understanding how CPTED dimensions shape public perceptions of safety. It also offers practical insights for the development of targeted public security policies. These findings suggest that CPTED principles can play a crucial role in reducing fear of crime and enhancing public safety in urban settings.
Semantic prosody of deictic verbs in the Holy Qurʾān: a corpus study
Authors: Al-Otaibi Ghuzayyil Mohammed 
Published: January 2025

Abstract
Studies that examine the semantic proprsody of words in the Qurʾān focus on words with negative or positive meanings as part of their denotations (i.e., core meanings). On the other hand, a few other studies place greater importance on collocates than on concordance lines or the pragmatic function of the phrase where the word occurs. Thus, the aim of the present study is to examine the semantic prosody of three groups of frequent deictic verbs in the Holy Qurʾān: (a) verbs denoting came as dʒaːʔ "Image missing", ħaˈḍara "Image missing", and ʔataː "Image missing", (b) those with the meaning of brought, such as dʒaːʔ (bi- "Image missing") "Image missing" and ʔataː (bi- "Image missing") "Image missing", and (c) verbs that mean sent (i.e., ˈnazzala "Image missing", ˈʔanzala "Image missing", ʔarsala "Image missing", baʕaθa "Image missing"). Some of the examined verbs are near synonyms, while others share the same root or word pattern. The study is qualitative and quantitative based on two corpora (i.e., the Qurʾānic Arabic Corpus and the Qurʾān Annotated Corpus in Sketch Engine). For the quantitative part, I used a number of statistical measures to identify frequent collocates of deictic verbs, and for the qualitative part, I checked concordance lines to decide on the evaluative meaning associated with each deictic verb. Further, a statistical test of ANOVA was used to identify which deictic meaning is significantly associated with which attitudinal evaluation. Results reveal that more than half of the deictic verbs (e.g., dʒaːʔ "Image missing" and ʔataː "Image missing" as came and brought and ˈʔaħḍara"Image missing" as brought) are negative. Further, verbs based on the same root or giving the same meaning do not necessarily suggest the same semantic prosody. More importantly, accompanying words no matter how significantly they occur with certain verbs are not sufficient to impose a certain semantic prosody on the verb in a specific context. This indicates that semantic prosody is determined by intentions, contexts, pragmatic functions, collocates, and grammatical constructions. Therefore, it is recommended that researchers examine the semantic prosody of other deictic verbs and analyse the role of prepositions following deictic verbs in determining the semantic prosody of verbs in the Qurʾān.
Study of a 2DOF bistable energy harvester with variable potential well for ultra-low frequency vibration energy harvesting
Authors: Chen Jun , Guan Mingjie , Shen Ziqiao , Zhu Qingyuan , Ning Yunbo 
Published: January 2025

A two-degree-of-freedom bistable energy harvester with a spring–magnet oscillator designed for ultra-low frequency vibration energy harvesting is presented in this paper. It combines magnetic plucking frequency upconversion and a variable potential function to achieve a high-efficiency response while also being suitably installed for applications with spatial limitations. A lumped parameter model of the piezoelectric energy harvester and the magnetic dipoles is applied to develop the theoretical model for the system. The prototype is fabricated, and experimental parameter investigations are undertaken to examine the effects of various factors, including magnetic distance, stiffness, and tip mass. The results indicate that at a frequency of 1.2 Hz and an acceleration of 0.23 g, the maximum output power reaches 264.6 µW at an optimal resistance of 250 kΩ.
Soil properties and environmental factors across different altitudes influence biodiversity of root endophytic fungi in the orchid Bletilla sinensis
Authors: Zhao Manli , Deng Wenxiang , Li Xue , Li Yongmei , Huang Qiong , Wang Chuntao 
Published: January 2025

Context Bletilla sinensis is a perennial orchid herb widely used in Chinese herbal medicine. The endophytic fungi associated with its roots significantly affect plant growth and secondary metabolite accumulation, whereas environmental factors crucially determine the community and diversity of these fungi. Aims This study investigated the impact of habitat environment on the symbiotic relationship between B. sinensis and its endophytic fungi by analysing the community composition and diversity of endophytic fungi in B. sinensis roots at various altitudes. Methods B. sinensis roots were sampled from three altitudes (HXJ1, HXJ2, HXJ3) in Jinjiang Town, Shangri-La County, Yunnan Province, China. Endophytic fungi were isolated and analysed using high-throughput sequencing technology. Key results The endophytic fungi were dominated by Ascomycota (85.4%) and Basidiomycota (14.6%). α-Diversity analysis showed that HXJ2 had the highest species richness (Chao1 = 34), while HXJ1 exhibited the highest diversity (Shannon = 2.25, Simpson = 0.84). β-Diversity analysis revealed moderate community similarities between sampling sites (Bray–Curtis indices: 0.433–0.597). Notably, fungal abundance showed strong positive correlations with soil total phosphorus (r = 1.000, P < 0.01) and total carbon (r = 0.998, P < 0.05) in HXJ1. Conclusions The study revealed a clear altitudinal pattern, where higher altitudes exhibited lower fungal diversity. However, the mid-altitude site (HXJ2) showed higher fungal diversity compared with both higher (HXJ3) and lower altitudes (HXJ1), suggesting that local environmental factors, such as soil nutrients and surrounding vegetation, may interact with temperature to influence fungal diversity. Implications This study sheds light on the symbiotic relationship between B. sinensis and its endophytic fungi, with implications for the cultivation and medicinal properties of this herb.
Spikes and spines in 3D Lorentzian simplicial quantum gravity
Authors: Borissova Johanna , Dittrich Bianca , Qu Dongxue , Schiffer Marc 
Published: January 2025

Abstract
Simplicial approaches to quantum gravity such as Quantum Regge Calculus and Spin Foams include configurations where bulk edges can become arbitrarily large while keeping the lengths of the boundary edges small. Such configurations pose significant challenges in Euclidean Quantum Regge Calculus, as they lead to infinities for the partition function and length expectation values. Here we investigate such configurations in three-dimensional Lorentzian Quantum Regge Calculus, and find that the partition function and length expectation values remain finite. This shows that the Lorentzian approach can avoid a key issue of the Euclidean approach. We also find that the space of configurations, for which bulk edges can become very large, is much richer than in the Euclidean case. In particular, it includes configurations with irregular light-cone structures, which lead to imaginary terms in the Regge action and branch cuts along the Lorentzian path integral contour. Hence, to meaningfully define the Lorentzian Regge path integral, one needs to clarify how such configurations should be handled.
Corrigendum: solution and solubility of H atoms at the W/Cu interface (2024 J. Phys.: Condens. Matter 36 465001)
Authors: Silva-Solís Y , Denis J , Hodille E A , Ferro Y 
Published: January 2025

Abstract
Metallic interfaces are locations where hydrogen (H) is expected to segregate and lead to the formation and stabilization of defects. This work focuses on the tungsten/copper (W/Cu) interface built according to the

W

bcc

(
001
)

/

Cu

hcp

(
11

2
¯

0
)

orientation. H behavior is subsequently determined at the interface and in its vicinity with electronic structure calculations based on the density functional theory. The electronic and vibrational properties determined in this way followed a thermodynamic treatment to deliver the solubility of H as a function of the temperature and chemical potential. The 96 interstitial positions we investigated reveal that H predominantly occupies the octahedral (Oh) sites in the copper network. Reversely, H exclusively occupies the tetrahedral (Td) sites in the tungsten network. The solubility of H is higher in the interface plane where both octahedral and tetrahedral sites are occupied. Despite this work is a first step toward kinetic modeling of hydrogen transport across the W/Cu interface, we conclude that the

W

bcc

(
001
)

/

Cu

hcp

(
11

2
¯

0
)

would behave like a sink where hydrogen isotopes could accumulate.
A novel compression torsion coupling metamaterial with repeatable energy dissipation characteristics
Authors: Lv Weitao , Li Dong 
Published: January 2025

Abstract
Energy dissipation metamaterials based on frictional energy dissipation have attracted much attention due to their reusable properties. The innovation of this paper is to propose a compression torsion coupling (CTC) metamaterial that can dissipate energy in the elastic stage by introducing the frictional energy dissipation mechanism into the CTC metamaterial. The proposed CTC structure was composed of rotating spring structures with Z-shaped ribs. 3D printing technology was used to make specimens for loading–unloading experiments. The mechanical properties were studied by finite element simulation and experiments. The influences of geometric parameters such as the helicity of the cylinder, the thickness of the cylinder and the thickness of the ribs on mechanical properties were studied. The results indicated that the proposed structure retains the CTC characteristic and can dissipate energy within the elastic range. Repeated loading–unloading experiments demonstrated that the proposed structure has good recoverability. The proposed CTC energy dissipation metamaterial was expected to be used in novel reusable buffer protection devices such as the landing gear of recyclable rockets.
Optimal placement of high-channel visual prostheses in human retinotopic visual cortex
Authors: van Hoof Rick , Lozano Antonio Manuel , Wang Feng , Klink Peter Christiaan , Roelfsema Pieter , Goebel Rainer 
Published: January 2025

Abstract
Objective:&#xD;Recent strides in neurotechnology show potential to restore vision in individuals afflicted with blindness due to early visual pathway damage. As neuroprostheses mature and become available to a larger population, manual placement and evaluation of electrode designs becomes costly and impractical. An automatic method to optimize the implantation process of electrode arrays at large-scale is currently lacking.&#xD;&#xD;Approach:&#xD;Here, we present a comprehensive method to automatically optimize electrode placement for visual prostheses, with the objective of matching pre-defined phosphene distributions. Our approach makes use of retinotopic predictions combined with individual anatomy data to minimize discrepancies between simulated and target phosphene patterns. While demonstrated with a 1000-channel 3D electrode array in V1, our pipeline is versatile, potentially accommodating any electrode design and allowing for design evaluation.&#xD;&#xD;Main results:&#xD;Notably, our results show that individually optimized placements in 362 brain hemispheres outperform average brain solutions, underscoring the significance of anatomical specificity. We further show how virtual implantation of multiple individual brains highlights the challenges of achieving full visual field coverage owing to single electrode constraints, which may be overcome by introducing multiple arrays of electrodes. Including additional surgical considerations, such as intracranial vasculature, in future iterations could refine the optimization process.&#xD;&#xD;Significance:&#xD;Our open-source software streamlines the refinement of surgical procedures and facilitates simulation studies, offering a realistic exploration of electrode configuration possibilities.
Nonuniversality in random criticality
Authors: Delfino Gesualdo 
Published: January 2025

Abstract
We consider N two-dimensional Ising models coupled in the presence of quenched disorder and use scale invariant scattering theory to exactly show the presence of a line of renormalization group fixed points for any fixed value of N other than 1. We show how this result relates to perturbative studies and sheds light on numerical simulations. We also observe that the limit N → 1 may be of interest for the Ising spin glass, and point out the potential relevance for nonuniversality in other contexts of random criticality.