bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine learning unveils RNA polymerase II binding as a predictor for SMAD2‐dependent transcription dynamics in response to Actvin signalling
Authors: Shi Dan , Feng Weihua , Zi Zhike 
Published: January 2024

AbstractThe transforming growth factor‐β (TGF‐β) superfamily, including Nodal and Activin, plays a critical role in various cellular processes. Understanding the intricate regulation and gene expression dynamics of TGF‐β signalling is of interest due to its diverse biological roles. A machine learning approach is used to predict gene expression patterns induced by Activin using features, such as histone modifications, RNA polymerase II binding, SMAD2‐binding, and mRNA half‐life. RNA sequencing and ChIP sequencing datasets were analysed and differentially expressed SMAD2‐binding genes were identified. These genes were classified into activated and repressed categories based on their expression patterns. The predictive power of different features and combinations was evaluated using logistic regression models and their performances were assessed. Results showed that RNA polymerase II binding was the most informative feature for predicting the expression patterns of SMAD2‐binding genes. The authors provide insights into the interplay between transcriptional regulation and Activin signalling and offers a computational framework for predicting gene expression patterns in response to cell signalling.
Adapting hip arthroplasty practices during the COVID-19 pandemic: Assessing the impact of outpatient care sudden increase on early complications and clinical outcomes
Authors: Foissey Constant , Pineda Tomas , Servien Elvire , Fontalis Andreas , Batailler Cécile , Lustig Sébastien 
Published: January 2024

Introduction: The COVID-19 pandemic has significantly affected access to timely care for patients with hip osteoarthritis requiring total hip replacement (THR). This study aimed to assess the changes in surgical activity, outpatient treatment, length of stay (LOS), discharge destinations, readmission rates, clinical outcomes, and patient satisfaction before and after the pandemic at our institution. Materials and methods: This retrospective study encompassed patients undergoing primary THR through the direct anterior approach at a single university hospital. Data on demographic characteristics, surgical technique, perioperative management, LOS, discharge destinations, complications, and clinical outcomes were collected. Furthermore, a comparative analysis between the pre-pandemic (2019) and post-pandemic (2022) periods was conducted. Results: There was a 14% increase in surgical activity post-pandemic, with 214 patients undergoing surgery in 2019 versus 284 in 2022. The percentage of patients managed as outpatients significantly increased from 0.5% in 2019 to 29.6% in 2022 (p < 0.001). LOS decreased from 2.7 ± 1 [0–8] days to 1.4 ± 1.1 [0–12] days (p < 0.001), and the rate of discharge to rehabilitation centres declined from 21.5% to 8.8% (p < 0.001). No significant increase in the readmission rates was observed (1.4% in both periods). At two months postoperatively, the mean HHS and satisfaction rates were comparable between the two groups (p = 1 and p = 0.73, respectively). Discussion: Despite the challenges posed by the COVID-19 pandemic, surgical activity at our institution demonstrated an increase compared to the pre-pandemic levels by expanding outpatient care, reducing LOS, and increasing rates of home discharges. Importantly, these changes did not adversely affect rehospitalization rates or early clinical outcomes.
Level of evidence: IV
In Vivo Kinematic Analysis of Mobile-Bearing Unicompartmental Knee Arthroplasty during High Flexion Activities
Authors: Kono Kenichi , Yamazaki Takaharu , Tamaki Masashi , Inui Hiroshi , Tanaka Sakae , Tomita Tetsuya 
Published: January 2024

AbstractMobile-bearing (MB) unicompartmental knee arthroplasty (UKA) has high conformity between the femoral articular surface and the meniscal bearing; therefore, the surface and subsurface contact stress is reduced. Additionally, the survival rate is high. However, the in vivo kinematics of MB UKA knees during high-flexion activities of daily living remain unknown. The aim of this study was to investigate in vivo the three-dimensional kinematics of MB UKA knees during high-flexion activities of daily living. A total of 17 knees of 17 patients who could achieve kneeling after MB UKA were examined. Under fluoroscopy, each patient performed squatting and kneeling motions. To estimate the spatial position and orientation of the knee, a two-dimensional/three-dimensional registration technique was used. We evaluated the femoral rotation and varus–valgus angle relative to the tibia and the anteroposterior translation of the medial sulcus (medial side) and lateral epicondyle (lateral side) of the femur on the plane perpendicular to the tibial mechanical axis in each flexion angle. From 130° to 140° of flexion, the femoral external rotation during squatting was significantly smaller than that during kneeling. Additionally, the medial side of the femur during squatting was significantly more posteriorly located compared with that during kneeling. There was no significant difference between squatting and kneeling in terms of the lateral side of the femur and the varus–valgus position in each flexion angle. At high flexion angle, the kinematics of MB UKA knees may differ depending on the performance.
Time analysis of online consumer behavior by decision trees, GUHA association rules, and formal concept analysis
Authors: Pitka Tomáš , Bucko Jozef , Krajči Stanislav , Krídlo Ondrej , Guniš Ján , Šnajder Ľubomír , Antoni Ľubomír , Eliaš Peter 
Published: January 2024

AbstractData analytics plays a significant role within the context of the digital business landscape, particularly concerning online sales, aiming to enhance understanding of customer behaviors in the online realm. We review the recent perspectives and empirical findings from several years of scholarly investigation. Furthermore, we propose combining computational methods to scrutinize online customer behavior. We apply the decision tree construction, GUHA (General Unary Hypotheses Automaton) association rules, and Formal concept analysis for the input dataset of 9123 orders (transactions) of sports nutrition, healthy foods, fitness clothing, and accessories. Data from 2014 to 2021, covering eight years, are employed. We present the empirical discoveries, engage in a critical discourse concerning these findings, and delineate the constraints inherent in the research process. The decision tree for classification of the year’s fourth quarter implies that the most important attributes are country, gross profit category, and delivery. The classification of the morning time implies that the most important attributes are gender and country. Thus, the potential marketing strategies can include heterogeneous conditions for men and women based on these findings. Analyzing the identified groups of customers by concept lattices and GUHA association rules can be valuable for targeted marketing, personalized recommendations, or understanding customer preferences.
The role of perceptual salience in a strengthening sound change
Authors: Denbaum-Restrepo Nofiya , Raynor Eliot 
Published: January 2024

Abstract
The current study examines the extent to which perceptual factors may account for the emergence of assibilated
variants of the alveopalatal approximant /j/ in two geographically remote varieties of Spanish. Participants from Medellin,
Colombia and Santiago, Dominican Republic completed a discrimination task and a matched guise. Both tasks presented listeners with
stimuli containing affricate [ʤ] and approximant [j] allophones of /j/. Participants were more accurate when discriminating
between sound pairs that included the affricate allophone, suggesting that the presence of (af)frication is a salient acoustic cue
upon which judgments are reliably made. Therefore, we argue that the emergence of assibilated variants ([ʤ], [ʒ]) can be explained
in part by more prominent acoustic cuing and thus greater perceptual salience. Evidence of the relationship between these findings
and a possible sound change in progress is observed in the association of social characteristics with [ʤ] and [j].
Management of Herbicide-Resistant Common Lambsquarters,
Chenopodium album
, in Atlantic Canada
Authors: McKenzie-Gopsill Andrew 
Published: January 2024

Abstract

Common lambsquarters (
Chenopodium album
) is one of the most troublesome annual broadleaf weeds in potato production in Atlantic Canada. Historically controlled by a combination of cultivation and herbicides, potato producers now often rely on a single herbicide application to provide season long control of common lambsquarters. The reliance on a few herbicides for common lambsquarters control has led to widespread incidence of herbicide resistance in this species, negatively impacting potato yield. This case study explores common lambsquarters’ biology, development of herbicide resistance, and the evolving management practices including chemical, cultural, and mechanical to help mitigate its impact on potato productivity.

Information
© His Majesty the King in Right of Canada as represented by the Minister of Agriculture and Agri-Food Canada, 2024
Integration of metabolomics and transcriptomics reveals the regulation mechanism of the phenylpropanoid biosynthesis pathway in insect resistance traits in Solanum habrochaites
Authors: Wang Meiliang , Wang Yudan , Li Xinzhi , Zhang Yao , Chen Xiuling , Liu Jiayin , Qiua Youwen , Wang Aoxue 
Published: January 2024

Abstract
Solanum habrochaites (SH), a wild species closely related to ‘Ailsa Craig’ (AC), is an important germplasm resource for modern tomato breeding. Trichomes, developed from epidermal cells, have a role in defense against insect attack, and their secretions are of non-negligible value. Here, we found that the glandular heads of type VI trichomes were clearly distinguishable between AC and SH under cryo-scanning electron microscopy, the difference indicating that SH could secrete more anti-insect metabolites than AC. Pest preference experiments showed that aphids and mites preferred to feed near AC compared with SH. Integration analysis of transcriptomics and metabolomics data revealed that the phenylpropanoid biosynthesis pathway was an important secondary metabolic pathway in plants, and SH secreted larger amounts of phenylpropanoids and flavonoids than AC by upregulating the expression of relevant genes in this pathway, and this may contribute to the greater resistance of SH to phytophagous insects. Notably, virus-induced silencing of Sl4CLL6 not only decreased the expression of genes downstream of the phenylpropanoid biosynthesis pathway (SlHCT, SlCAD, and SlCHI), but also reduced resistance to mites in tomato. These findings provided new genetic resources for the synthesis of phenylpropanoid compounds and anti-insect breeding in S. habrochaites and a new theoretical basis for the improvement of important traits in cultivated tomato.
Is there an association between gender stereotypes and sexual risk attitudes and behaviors? A population-based study among Peruvian adolescents
Authors: Ticona Diana Manuela , Musaja-Cruz Ariana Gabriela , Durand-Anahua Paula Regina , Escobar-Cabezas Raul Eduardo , Mamani Luz Mirian , Lopez Crislee Elizabeth 
Published: January 2024

Abstract

Background
Adolescents, particularly those aged 14 to 15 y, often begin exploring their sexuality, during which time they are more vulnerable to traditional influences and ideologies imposed by society. This study aimed to identify the association between more traditional attitudes toward women and sexual risk attitudes and behaviors in Peruvian adolescents.

Materials and methods
Cross-sectional study with data from the fifth round of the Young Lives study with 1860 adolescents aged 14 and 15 y. Population characteristics were described by relative frequencies and using chi-squared test with p-value. The 12 items of the Attitudes toward Women Scale for Adolescents instrument were analyzed by relative frequencies and mean with standard deviation. For further analysis, the values of the global scores of all participants were divided into two categories, using the median as the cutoff point, where the group with higher scores indicated that these adolescents had more traditional attitudes (or more gender stereotypes). More traditional attitudes were associated with sexual risk attitudes and behaviors, with crude prevalence ratio (PR) and then adjusted prevalence ratio (aPR) with 95% CI. The Universidad Privada de Tacna’s ethics committee approved the research protocol.

Results
Adolescents with more traditional attitudes were more likely to have sexual risk attitudes compared to those with less traditional attitudes. In addition, adolescents with more traditional attitudes were 2.6 times more likely to have at least one sexual intercourse while drunk as compared to the reference group (95% CI: 1.43–4.74; p=0.002).

Conclusions
The expression of more traditional attitudes toward women was associated with sexual risk attitudes. However, there was no association with most of the sexual risk behaviors studied, except for the higher probability of having at least one sexual intercourse while drunk.
Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis
Authors: Liu Wei , Dou Zhen , Wang Chunyue , Zhao Gangyin , Wu Fengge , Wang Chunli , Aikhionbare Felix , Ye Mingliang , Sedzro Divine Mensah , Yang Zhenye , Fu Chuanhai , Wang Zhikai , Gao Xinjiao , Yao Xuebiao , Song Xiaoyu , Liu Xing 
Published: January 2024

Abstract
Accurate chromosome segregation in mitosis depends on kinetochores that connect centromeric chromatin to spindle microtubules. Centromeres are captured by individual microtubules via a kinetochore constitutive centromere-associated network (CCAN) during chromosome segregation. CCAN contains 16 subunits, including CENP-W and CENP-T. However, the molecular recognition and mitotic regulation of the CCAN assembly remain elusive. Here, we revealed that CENP-W binds to the histone fold domain and an uncharacterized N-terminal region of CENP-T. Aurora B phosphorylates CENP-W at threonine 60, which enhances the interaction between CENP-W and CENP-T to ensure robust metaphase chromosome alignment and accurate chromosome segregation in mitosis. These findings delineate a conserved signaling cascade that integrates protein phosphorylation with CCAN integrity for the maintenance of genomic stability.
Edward Sapir
Authors: Stanlaw James 
Published: January 2024

Edward Sapir (b. 1884–d. 1939) is one of the most important figures in the history of American linguistics and anthropology before the Second World War. Both disciplines would likely be drastically different today had Sapir’s influence not been felt. He was recognized by his contemporaries as the finest Americanist (specialist on the languages of Native America) of his day. His investigations of Takelma, Paiute, Yana, and Wishram are definitive, and his work on other Native American languages—from Athabaskan to Uto-Aztecan—really have not been superseded. His work on phonetic transcription, and theoretical and practical phonemics, was seminal. His curiosity and wide-ranging explorations touched on almost all aspects of linguistics, and some of his ideas were quite prescient. For example, Sapir’s work on sound symbolism—largely neglected at the time (1929)—has recently received renewed attention. Sapir is also thought by anthropologists to be one of the discipline’s most original and profound theorists: He did groundbreaking work on culture and personality studies, cultural psychology, and the relationship between the individual and culture. He continues to be widely read. Together with Franz Boas—often said to be the father of American anthropology, and who instigated the field at the turn of the 20th century—Sapir insured that linguistics—especially how language relates to culture and thought—would be one of the new discipline’s four primary subfields. Indeed, debates on “linguistic relativity,” or the so-called Sapir-Whorf Hypothesis—the degrees and ways language may affect thought, experience, or perception—even today occupy the professional time of many in linguistics, anthropology, psychology, philosophy, and cognitive science. These issues are often contentious, and even have made their appearance in popular culture. Sapir was president of both the Linguistic Society of America (1933) and the American Anthropological Association (1938), an obvious indication of his stature in both fields.
The phase separation of extracellular matrix protein matrilin‐3 from cancer‐associated fibroblasts contributes to gastric cancer invasion
Authors: Huang Yuliang , Xu Xiaoyang , Lu Yunkun , Sun Qiang , Zhang Lu , Shao Jiaqi , Chen Dingwei , Chang Yongxia , Sun Xiaoxia , Zhuo Wei , Zhou Tianhua 
Published: January 2024

AbstractCancer‐associated fibroblast (CAF) has emerged as a key contributor to the remodeling of tumor microenvironment through the expression and secretion of extracellular matrix (ECM) proteins, thereby promoting carcinogenesis. However, the precise contribution of ECM proteins from CAFs to gastric carcinogenesis remains poorly understood. In this study, we find that matrilin‐3 (MATN3), an upregulated ECM protein associated with poorer prognosis in gastric cancer patients, originates from CAFs in gastric cancer tissues. Ectopic expression of MATN3 in CAFs significantly promotes the invasion of gastric cancer cells, which can be attenuated by neutralizing MATN3 with its antibody. Notably, a portion of MATN3 protein is found to form puncta in gastric cancer tissues ECM. MATN3 undergoes phase separation, which is mediated by its low complexity (LC) and coiled‐coil (CC) domains. Moreover, overexpression of MATN3 deleted with either LC or CC in CAFs is unable to promote the invasion of gastric cancer cells, suggesting that LC or CC domain is required for the effect of CAF‐secreted MATN3 in gastric cancer cell invasion. Additionally, orthotopic co‐injection of gastric cancer cells and CAFs expressing MATN3, but not its ΔLC and ΔCC mutants, leads to enhanced gastric cancer cell invasion in mouse models. Collectively, our works suggest that MATN3 is secreted by CAFs and undergoes phase separation, which promotes gastric cancer invasion.
α‐Catenin promotes dermal fibroblasts proliferation and migration during wound healing via FAK/YAP activation
Authors: Wang Nan , Xu Xiejun , Guan Fangqian , Zheng Yeyi , Shou Yanni , Xu Tianpeng , Shen Guoxiu , Chen Hui , Lin Yifan , Cong Weitao , Jin Litai , Zhu Zhongxin 
Published: January 2024

AbstractSkin wound healing is a complex and organized biological process, and the dermal fibroblasts play a crucial role. α‐Catenin is known to be involved in regulating various cellular signals, and its role in wound healing remains unclear. Here, we have identified the pivotal role of the α‐catenin/FAK/YAP signaling axis in the proliferation and migration of dermal fibroblasts, which contributes to the process of skin wound healing. Briefly, when α‐catenin was knocked down specifically in dermal fibroblasts, the wound healing rate is significantly delayed. Moreover, interfering with α‐catenin can impede the proliferation and migration of dermal fibroblasts both in vitro and in vivo. Mechanistically, the overexpression of α‐catenin upregulates the nuclear accumulation of YAP and transcription of downstream target genes, resulting in enhanced the proliferation and migration of dermal fibroblasts. Furthermore, the FAK Tyr397 phosphorylation inhibitor blocked the promoting effects of α‐catenin on YAP activation. Importantly, the continuous phosphorylation mutation of FAK Tyr397 reversed the retardatory effects of α‐catenin knockdown on wound healing, by increasing the vitality of fibroblasts. Likewise, α‐catenin/FAK was validated as a therapeutic target for wound healing in the db/db chronic trauma model. In summary, our findings have revealed a novel mechanism by which α‐catenin facilitates the function of fibroblasts through the activity of the FAK/YAP signaling axis. These findings define a promising therapeutic strategy for accelerating the wound healing process.
Neuronavigation-Guided Percutaneous Rhizotomies to Trigeminal Neuralgia
Authors: Wu Felix Ho Won , Cheung Chi Wai , Leung Yiu Yan 
Published: January 2024

Objective:
Neuronavigation improves intraoperative visualisation to the cranial structures, which is valuable in percutaneous surgical treatments for trigeminal neuralgia (TN) patients who are refractory to pharmacotherapy or reluctant to receive open surgery. The objective of this review is to evaluate the available neuronavigation-guided percutaneous surgical treatment modalities with cannulation of foramen ovale to TN, and their relative benefits and limitations.

Methods:
This review was conducted based on the PRISMA statement. An initial search was performed on electronic databases, followed by manual and reference searches. Study and patient characteristics, rhizotomy procedure and neuronavigation details, and treatment outcomes (initial pain relief and pain recurrence within 2 years, success rate of forman ovale cannulation, and complications) were evaluated. Risk of bias was assessed with a quality assessment based on the ROBINS-I tools.

Results:
Ten studies (491 operations, 403 subjects) were analysed. Three percutaneous trigeminal rhizotomy modalities identified were radiofrequency thermocoagulation rhizotomy (RFTR), percutaneous balloon compression (PBC), glycerol rhizotomy. iCT/MRI RFTR had the highest initial pain relief rate of 97.0%. Success rate of FO cannulation ranged from 92.3% to 100% under neuronavigation. Facial hypoesthesia and masticatory muscle weakness were the most reported complications.

Discussion:
Neuronavigation-guided percutaneous trigeminal rhizotomies showed possible superior pain relief outcomes to that of conventional rhizotomies in TN, with the benefits of radiation reduction and lower complication development rates. Limitations of neuronavigation remain its high cost and limited availability. Higher-quality prospective studies and randomized clinical trials of neuronavigation-guided percutaneous trigeminal rhizotomy were lacking.
Competing Tensions
Authors: Carew Maddison , Redley Bernice , Bloomer Melissa J. 
Published: January 2024

Background:
Family-centered care is founded upon collaboration between parents and healthcare professionals, caring for a child and parents as one entity. The unfamiliar neonatal environment and complexity of care can make family-centered care challenging.

Purpose:
To explore neonatal nurses' perceptions of family-centered care and parents' cultural needs.

Methods:
This was a qualitative descriptive study using interviews to gather data from registered nurses, and analyzed using inductive content analysis.

Results:
Ten neonatal nurses participated in online interviews, lasting an average of 25 minutes. Parents' cultural needs were poorly understood and assumed synonymous with family-centered care. While all acknowledged the importance of family-centered care, most described tasks to parent–infant bonding, rather than a broader embodiment of family-centered care. In time of uncertainty, emergent clinical priorities took priority over a family-centered approach to care. Cultural care was poorly understood, and care tasks associated with supporting parent–infant bonding suggest further work is necessary to promote embodiment of family-centered care beyond individual tasks. While emergent clinical priorities and neonate well-being will always be the priority, finding a way to respond that concords with the ethos of family-centered care is also essential.

Implications for Practice and Research:
Clear and consistent leadership is needed to demonstrate greater embodiment of family-centered care, which includes cultural care for parents. Strong leadership and targeted education are key to supporting this change. Further research is warranted to examine and observe practice, in particular how parents' cultural needs are assessed and integrated into family-centered care in neonatal settings.