bluuebunny/crossref_metadata_2025_split huggingface dataset:
Piezo1 agonist restores meningeal lymphatic vessels, drainage, and brain-CSF perfusion in craniosynostosis and aged mice
Authors: Matrongolo Matt J. , Ang Phillip S. , Wu Junbing , Jain Aditya , Thackray Josh K. , Reddy Akash , Sung Chi Chang , Barbet Gaëtan , Hong Young-Kwon , Tischfield Max A. 
Published: September 2023

AbstractSkull development coincides with the onset of cerebrospinal fluid (CSF) circulation, brain-CSF perfusion, and meningeal lymphangiogenesis, processes essential for brain waste clearance. How these processes are affected by craniofacial disorders such as craniosynostosis are poorly understood. We report that raised intracranial pressure and diminished CSF flow in craniosynostosis mouse models associates with pathological changes to meningeal lymphatic vessels that affect their sprouting, expansion, and long-term maintenance. We also show that craniosynostosis affects CSF circulatory pathways and perfusion into the brain. Further, craniosynostosis exacerbates amyloid pathology and plaque buildup inTwist1+/−:5xFADtransgenic Alzheimer’s disease models. Treating craniosynostosis mice with Yoda1, a small molecule agonist for Piezo1, reduces intracranial pressure and improves CSF flow, in addition to restoring meningeal lymphangiogenesis, drainage to the deep cervical lymph nodes, and brain-CSF perfusion. Leveraging these findings, we show Yoda1 treatments in aged mice with reduced CSF flow and turnover improve lymphatic networks, drainage, and brain-CSF perfusion. Our results suggest CSF provides mechanical force to facilitate meningeal lymphatic growth and maintenance. Additionally, applying Yoda1 agonist in conditions with raised intracranial pressure and/or diminished CSF flow, as seen in craniosynostosis or with ageing, is a possible therapeutic option to help restore meningeal lymphatic networks and brain-CSF perfusion.
Gas5 regulates early life stress-induced anxiety and spatial memory
Authors: Banerjee Dipanjana , Sultana Sania , Banerjee Sourav 
Published: September 2023

ABSTRACTEarly life stress (ES) induced by maternal separation (MS) remains a proven causality of anxiety and memory deficits at later stages of life. Emerging studies have shown that MS-induced gene expression in the hippocampus is operated at the level of transcription. However, the extent of involvement of non-coding RNAs in MS-induced behavioural deficits remains unexplored. Here, we have investigated the role of synapse-enriched long non-coding RNAs (lncRNAs) in anxiety and memory upon MS. We observed that MS led to an enhancement of expression of the lncRNA Gas5 (Growth Arrest Specific-5) in the hippocampus; accompanied by increased levels of anxiety and deficits in spatial memory. Gas5 knockdown in early life was able to reduce anxiety and partially rescue the spatial memory deficits of maternally separated adult mice. However, the reversal of MS-induced anxiety and memory deficits is not attributed to Gas5 activity during neuronal development as Gas5 RNAi did not influence spine development. Gene Ontology analysis revealed that Gas5 exerts its function by regulating RNA metabolism and translation. Our study highlights the importance of MS-regulated lncRNA in anxiety and spatial memory.
Circuit-based intervention corrects excessive dentate gyrus output in the Fragile X mouse model
Authors: Deng Pan-Yue , Kumar Ajeet , Cavalli Valeria , Klyachko Vitaly A. 
Published: September 2023

AbstractAbnormal cellular and circuit excitability is believed to drive many core phenotypes in fragile X syndrome (FXS). The dentate gyrus is a brain area performing critical computations essential for learning and memory. However, little is known about dentate circuit defects and their mechanisms in FXS. Understanding dentate circuit dysfunction in FXS has been complicated by the presence of two types of excitatory neurons, the granule cells and mossy cells. Here we report that loss of FMRP markedly decreased excitability of dentate mossy cells, a change opposite to all other known excitability defects in excitatory neurons in FXS. This mossy cell hypo-excitability is caused by increased Kv7 function inFmr1KO mice. By reducing the excitatory drive onto local hilar interneurons, hypo-excitability of mossy cells results in increased excitation/inhibition ratio in granule cells and thus paradoxically leads to excessive dentate output. Circuit-wide inhibition of Kv7 channels inFmr1KO mice increases inhibitory drive onto granule cells and normalizes the dentate output in response to physiologically relevant theta-gamma coupling stimulation. Our study suggests that circuit-based interventions may provide a promising strategy in this disorder to bypass irreconcilable excitability defects in different cell types and restore their pathophysiological consequences at the circuit level.
Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality control
Authors: Chen Katharine Y. , Park Heungwon , Subramaniam Arvind Rasi 
Published: September 2023

AbstractDecay of mRNAs can be triggered by ribosome slowdown at stretches of rare codons or positively charged amino acids. However, the full diversity of sequences that trigger co-translational mRNA decay is poorly understood. To comprehensively identify sequence motifs that trigger mRNA decay, we use a massively parallel reporter assay to measure the effect of all possible combinations of codon pairs on mRNA levels inS. cerevisiae. In addition to known mRNA-destabilizing sequences, we identify several dipeptide repeats whose translation reduces mRNA levels. These include combinations of positively charged and bulky residues, as well as proline-glycine and proline-aspartate dipeptide repeats. Genetic deletion of the ribosome collision sensor Hel2 rescues the mRNA effects of these motifs, suggesting that they trigger ribosome slowdown and activate the ribosome-associated quality control (RQC) pathway. Deep mutational scanning of an mRNA-destabilizing dipeptide repeat reveals a complex interplay between the charge, bulkiness, and location of amino acid residues in conferring mRNA instability. Finally, we show that the mRNA effects of codon pairs are predictive of the effects of endogenous sequences. Our work highlights the complexity of sequence motifs driving co-translational mRNA decay in eukaryotes, and presents a high throughput approach to dissect their requirements at the codon level.
LRRK2 kinase dependent and independent function on endolysosomal repair promotes macrophage cell death
Authors: Morrison Rebecca , Mihaylov Simeon R , Luk Chak Hon , Rodgers Angela , Athanasiadi Natalia , Morris Huw R , Pellegrino Enrica , Ultanir Sila , Gutierrez Maximiliano G 
Published: September 2023

AbstractLRRK2 is commonly mutated in Parkinson’s disease and has cell type-specific mechanisms of activation and function. In macrophages, LRRK2 is associated with lysosomes and is activated following lysosomal damage. However, effects of pathogenic LRRK2-G2019S in macrophages are unknown. Here, using primary mouse and human iPSC-derived macrophage (iPSDM) models of LRRK2-G2019S, we defined the substrates of LRRK2 after lysosomal damage. Using phosphoproteomics we found that LRRK2-G2019S and wild-type macrophages showed similar levels of Rab phosphorylation after lysosomal damage, with the exceptions of Rab12 and Rab35, which were increased and decreased, respectively, in LRRK2-G2019S. LRRK2-G2019S macrophages showed a LRRK2 kinase activity-independent deficit in lysosomal membrane repair which resulted in more cell death and increased apoptosis. Importantly, we recapitulated this phenotype in iPSDM from patients carrying the G2019S mutation, but not in isogenic control iPSDM. Altogether, we define here the signaling downstream of G2019S in macrophages and identify susceptibility to cell death after lysosomal damage as an important phenotype of this mutation.
Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability
Authors: McGinnis Madeline M. , Sutter Benjamin M. , Jahangiri Samira , Tu Benjamin P. 
Published: September 2023

AbstractAutophagy is a conserved process of cellular self-digestion that promotes survival during nutrient stress. In yeast, methionine starvation is sufficient to induce autophagy. One pathway of autophagy induction is governed by the SEACIT complex, which regulates TORC1 activity in response to amino acids through the Rag GTPases Gtr1 and Gtr2. However, the precise mechanism by which SEACIT senses amino acids and regulates TORC1 signaling remains incompletely understood. Here, we identify the conserved 5’-3’ RNA exonuclease Xrn1 as a surprising and novel regulator of TORC1 activity in response to methionine starvation. This role of Xrn1 is dependent on its catalytic activity, but not on degradation of any specific class of mRNAs. Instead, Xrn1 modulates the nucleotide-binding state of the Gtr1/2 complex, which is critical for its interaction with and activation of TORC1. This work identifies a critical role for Xrn1 in nutrient sensing and growth control that extends beyond its canonical housekeeping function in RNA degradation and indicates an avenue for RNA metabolism to function in amino acid signaling into TORC1.
Gamification and its challenges in e-learning: a case study of computer science learners in KKHSOU
Authors: Barua Antara Mahanta , Bharali Sruti Sruba 
Published: September 2023

PurposeThe purpose of the case study is to investigate the perception of computer science learners at Krishna Kanta Handiqui State Open University (KKHSOU) regarding the use of gamification and to identify the challenges in implementing gamified teaching-learning processes in open and distance learning (ODL) institutions.Design/methodology/approachThe case study was carried out by observing the views of the learners on the use of gamification in distance learning and studying the participation level of computer science learners before and after the gamification environment was added to a specific topic through the learning management system (LMS). The pre-game and post-game effects on learners' participation against various factors using t-tests were also investigated in this study.FindingsThe study reveals that gamification had a positive and highly significant influence on the learners' participation and engagement in the e-content provided via the LMS. The post-gamification mean score (5.62) was higher than the pre-gamification mean score (2.36), and the overall paired t-test (5.301) value was significant at the 1% level of significance. Some of the challenges faced during the study were communication with the learner, lack of technical skills of the learner, duration of study, etc.Originality/valueThis study will help in understanding the perception and effect of gamification on computer science learners in ODL mode in India. The study succeeded in demonstrating that the use of gamified elements results in increased learner participation.
Extending training predictors link with training transfer through mediation of motivation
Authors: Yaqub Yasmin , Dutta Tanusree , Singh Arun Kumar , Srivastava Abhaya Ranjan 
Published: September 2023

PurposeThe study proposes to empirically test a model that illustrates how identical elements (IEs), transfer design and trainer performance as training predictors affect trainees' motivation to improve work through learning (MTIWL) and training transfer (TT) in the Indian context.Design/methodology/approachAn online survey was conducted to validate the study model. The quantitative data collected from 360 executives and managers were analyzed using the covariance-based structural equation modeling (CB-SEM) technique.FindingsThe study finds that trainees' MTIWL has a full mediation impact between transfer design, trainer performance and TT. However, a partial mediating impact of MTIWL was found between IEs and TT.Originality/valueThis is the first study that empirically explores the mediating mechanism of MTIWL between IEs, transfer design, trainer performance and TT. This study extends the current understanding of trainees' MTIWL that links the cumulative influence of training predictors to TT.
Bounding gait control of a parallel quadruped robot
Authors: Hao Xu , Wei Lang , Qiao Yue , Xu Shengzui , Liao Jian Bin , Xi Yu , Wei Wang , Liu Zhi-Wei 
Published: September 2023

Purpose
The computing power of the legged robot is not enough to perform high-frequency updates for the full-body model predictive control (MPC) of the robot, which is a common problem encountered in the gait research of the legged robot. The purpose of this paper is to propose a high-frequency MPC control method for the bounding gait of a parallel quadruped robot.

Design/methodology/approach
According to the bounding gait characteristics of the robot, the quadruped robot model is simplified to an equivalent plane bipedal model. Under the biped robot model, the forces between the robot’s feet and the ground are calculated by MPC. Then, the authors apply a proportional differential controller to distribute these forces to the four feet of the quadruped robot. The robot video can be seen at www.bilibili.com/video/BV1je4y1S7Rn.

Findings
To verify the feasibility of the controller, a prototype was made, and the controller was deployed on the actual prototype and then fully analyzed through experiments. Experiments show that the update frequency of MPC could be stabilized at 500 Hz while the robot was running in the bounding gait stably and efficiently.

Originality/value
This paper proposes a high-frequency MPC controller under the simplified model, which has a higher working efficiency and more stable control performance.
I want what they have: the impact of salesperson envy on customer relationships amid organizational disruption
Authors: Hancock Tyler , Mallin Michael L. , Pullins Ellen B. , Johnson Catherine M. 
Published: September 2023

Purpose
This study aims to use cognitive appraisal theory to explain how organizational disruption influences the development of envy resulting in unethical selling practices, turnover intentions and a reduction in customer orientation that causes disruption to impact customer relationships. This research helps to address drivers of salesperson envy, the potential disruptions to customer relationships and the required need to invest in psychological resources to offset these negative effects.

Design/methodology/approach
A total of 211 salespeople were surveyed to test the hypotheses. First, the measurement model was validated using a confirmatory factor analysis. Next, the hypotheses were tested using structural equation modeling AMOS 27. Mediation and moderated mediation were tested using the bootstrap method. Estimands were created within AMOS to test the indirect and interaction effects in the full model. A post hoc analysis further informed the findings.

Findings
The results show that the development of envy increases under conditions of organizational disruptions, leading to potential customer disruptions through turnover intentions, unethical selling behaviors and a reduction in customer orientation. In addition, the mediation analysis shows that envy drives the relationship between organizational disruption and unethical selling, turnover intentions and customer orientation through fully mediated relationships. Finally, the interaction effects between organizational disruption and psychological capital show high levels of psychological capital help to decrease the development of envy, thus reducing unethical selling behaviors and turnover intentions while increasing customer orientation.

Practical implications
The study provides practitioners with insights into how to reduce envy by investing in the psychological capital of their salesforce. The study also provides suggestions for handling disruptions and managing envy to prevent actions that act to damage customer relationships.

Originality/value
Salespeople are likely to encounter organizational disruption. Sales managers need to be prepared to manage the outcomes of organizational disruption as it impacts the sales force. Understanding how disruptions impact customer relationships through envy is an important yet under-explored topic. This research adds to and expands the sales literature using cognitive appraisal theory to help address drivers of salesperson envy and its potentially negative impact on customer relationships and shows the required need to invest in psychological resources to offset these negative effects. The study also helps expand the recent focus on worldwide disruptions by adopting another context for disruption stemming from organizational disruption.
Frequency‐domain autocorrelation imaging condition for 3D elastic time‐reversal imaging
Authors: Yoon Byoungjoon , Moon Seokjoon , Cho Yongchae , Park Kwon‐Gyu , Pyun Sukjoon 
Published: September 2023

AbstractIn this study, we propose a frequency‐domain autocorrelation as an imaging condition for time‐reversal imaging. The previous imaging conditions in time‐reversal imaging require time‐domain calculations such as inverse Fourier transform. The computational burden of these calculations is critical; therefore, time‐reversal imaging is not the proper method for real‐time event localization. We exclude these time‐domain calculations for efficiency. Instead, the maximum amplitude position of the frequency‐domain autocorrelation of the time‐reversal wavefield is determined as a source location. We conducted numerical tests to validate our imaging condition. The synthetic data test shows that our proposed algorithm provides a more credible source localization result than the conventional grid‐search method does in a noisy environment even using only one frequency component with many receivers. We also applied our algorithm to two real datasets acquired from the small monitoring network in Pohang. The real‐data test shows a comparable result with the result of the grid‐search method.
Modelled economic evaluation of a virtual emergency department in Victoria
Authors: Le Long Khanh‐Dao , Thai Thao , Cameron Peter A , Sri‐Ganeshan Muhuntha , O'Reilly Gerard M , Mitra Biswadev , Nehme Ziad , Brichko Lisa , Underhill Andrew , Charteris Claire , Egerton‐Warburton Diana , Mihalopoulos Cathrine 
Published: September 2023

AbstractObjectiveVirtual ED (VED) can potentially alleviate ED overcrowding which has been a public health challenge. The aim of the present study was to conduct a return‐on‐investment analysis of a VED programme developed in response to changing healthcare needs in Australia.MethodsAn economic model was developed based on initial patient outcome data to assess the healthcare costs, potential costs saved and return on investment (ROI) from the VED. The VED programme operating as part of Alfred Health Emergency Services. The participants were the first 188 patients accessing the Alfred Health VED. VED is the delivery of emergency assessment and management of specific patients virtually via audio‐visual teleconferencing. ROI ratios that compare cost savings with intervention costs.ResultsThe mean total operational cost of VED for 79 days for 188 patients was A$344 117 (95% uncertainty interval [UI] $296 800–$392 088). The VED led to a potential A$286 779 (95% UI $241 688–$330 568) healthcare cost saving from reductions in emergency visits and A$97 569 (95% UI $74 233–$123 117) cost saving in ambulance services. The ROI ratio was estimated at 1.12 (95% UI 0.96–1.32).ConclusionsThe VED was cost neutral in a conservatively modelled scenario but promising if any hospital admission could be saved. Ongoing research examining a larger cohort with community follow up is required to confirm this promising result.
Do rights matter? An intraday analysis of rights issues in Australia
Authors: Drienko Jozef , Sault Stephen J. , Wong Wai Han 
Published: September 2023

AbstractWe examine intraday abnormal returns associated with rights issue announcements in the Australian equity market over the period January 2000 to December 2022. Consistent with prior studies, we find significant abnormal returns ranging from −2.9% to −2.7% on the event day. We provide the first evidence on intraday price reactions pertaining to rights issues in Australia. Within 15 min of an announcement, we find significant abnormal trade returns of −1.2%. However, market participants are unable to profit by trading on these announcements due to transaction costs. Our results imply that the information content is fully impounded within 90 min.
Letter: NSBB for fatty liver disease—Still a long way to go. Authors' reply
Authors: Jachs Mathias , Paternostro Rafael , Reiberger Thomas , Mandorfer Mattias 
Published: September 2023

LINKED CONTENTThis article is linked to Paternostro et al papers. To view these articles, visit https://doi.org/10.1111/apt.17653 and https://doi.org/10.1111/apt.17692
Challenging the geographic bias in recognising large‐scale patterns of diversity change
Authors: Zhang Wenyuan , Grenyer Richard , Gaston Kevin J. , Sheldon Ben C. 
Published: September 2023

AbstractAimGeographic structure is a fundamental organising principle in ecological and Earth sciences, and our planet is conceptually divided into distinct geographic clusters (e.g. ecoregions and biomes) demarcating unique diversity patterns. Given recent advances in technology and data availability, however, we ask whether geographically clustering diversity time‐series should be the default framework to identify meaningful patterns of diversity change.LocationNorth America.TaxonAves.MethodsWe first propose a framework that recognises patterns of diversity change based on similarities in the behaviour of diversity time‐series, independent of their specific or relative spatial locations. Specifically, we applied an artificial neural network approach, the self‐organising map (SOM), to group time‐series of over 0.9 million observations from the North American Breeding Birds Survey (BBS) data from 1973 to 2016. We then test whether time‐series identified as having similar behaviour are geographically structured.ResultsWe find little evidence of strong geographic structure in patterns of diversity change for North American breeding birds. The majority of the recognised diversity time‐series patterns tend to be indistinguishable from being independently distributed in space.Main ConclusionsOur results suggest that geographic proximity may not correspond to shared temporal trends in diversity; assuming that geographic clustering is the basis for analysis may bias diversity trend estimation. We suggest that approaches that consider variability independently of geographic structure can serve as a useful addition to existing organising rules of biodiversity time‐series.
Characterization of Lasiodiplodia theobromae (Pat.) Griffon & Maubl causing stem canker on Teak (Tectona grandis L.) in Ecuador
Authors: Vélez‐Zambrano Sergio Miguel , Vélez‐Olmedo Jefferson Bertin , García Susana , Bonfim Bianca S. A. , Cedeño Pedro Emilio , Pinho Danilo 
Published: September 2023

AbstractTeak is a very important tropical timber in Ecuador. In 2017, teak samples displaying stem canker symptoms were collected in two provinces of Ecuador. From 11 symptomatic trees, 11 isolates resembling a species of Lasiodiplodia were obtained. All isolates obtained induced stem canker on teak plants after artificial inoculation, confirming them as the cause of the observed canker symptoms. Bayesian inference with concatenated sequences of complete ITS and partial TEF‐α and β‐TUB gene sequences from two representative isolates clustered teak isolates with other sequences of Lasiodiplodia theobromae available in Genbank. This is the first report of L. theobromae causing stem canker on teak plants in Ecuador.