bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemo-sensitive and chemo-resistant ovarian cancer cells show differences in cellular processes leading to pyroptotic cell death
Authors: Berkel Caglar , CACAN Ercan 
Published: October 2023

Abstract
Tumor immunology in ovarian cancer is not completely understood. Chemoresistance limits the success of available treatment options for patients with ovarian cancer. Pyroptosis, pro-inflammatory programmed cell death characterized by membrane pore formation by gasdermin proteins, is important for both immunogenicity and drug resistance. Here, we showed that estrogen increases GSDMC and GSDMD mRNA levels in chemo-sensitive ovarian cancer cells but not in chemo-resistant ovarian cancer cells in vitro. GSDMC or GSDMD overexpression increases cell viability in chemo-sensitive ovarian cancer cells but not in chemo-resistant ovarian cancer cells. Silencing of GSDMD in chemo-sensitive ovarian cancer cells and silencing of GSDMC in chemo-resistant ovarian cancer cells limits the effect of nigericin, a pyroptosis inducer, on cell viability. Inhibition of caspase-1, -4, -6 or -8 blocks nigericin-induced cell death (pyroptosis) in chemo-sensitive ovarian cancer cells; however, only the inhibition of caspase-1 blocks nigericin-induced cell death in chemo-resistant ovarian cancer cells, showing that caspases participating in pyroptosis might differ between ovarian cancer cells based on their chemo-sensitivity profiles. Treatment with disulfiram, a GSDMD pore formation inhibitor, decreases cell viability in both cell lines. Lastly, we found that in chemo-resistant ovarian cancer cell line, disulfiram and nigericin combination treatment decreased cell viability even more compared to only disulfiram or only nigericin treatment. Combined, our study pointed that ovarian cancer cells with different chemosensitivity profiles might have certain differences in pyroptotic cell death.
Deciphering Hydrochemical Characteristics and Formation mechanism of Groundwater in the Banggou Iron Mine Area, Peru
Authors: Ge Qin , Wang Huiman , Wan Sheng , Wu Ping , Li Xiang , Mi Zhenhua , Li Xinyan , Wang Yang 
Published: October 2023

Abstract
Understanding the hydrochemical characteristics and formation processes of groundwater in mining areas is crucial for protecting and utilizing underground water resources. This study analyzed 42 groundwater samples from the Banggou mining area in Peru to determine the spatial-temporal distribution patterns and formation processes of chemical components. Statistical analysis, Gibbs diagrams, ion ratios, and hydrogeochemical modeling techniques were used. The results show that the groundwater in the study area is weakly alkaline (pH = 7.72) and has a total dissolved solids (TDS) range of 253.19 to 17912.82 mg/L, with an average of 2788.31 mg/L. About 55% of the groundwater samples are classified as brackish or saline water. The chemical composition of groundwater is mainly controlled by evaporation, rock weathering, human activities, oxidation of pyrite, and desulfurization reactions. The dissolution of evaporite minerals such as halite and gypsum contributes to the presence of Na+, Cl−, and SO42−, while Ca2+ and Mg2+ mainly come from the dissolution of minerals like calcite, gypsum, dolomite, and silicate. The presence of NO3− is attributed to anthropogenic influences. The hydrogeochemical modeling indicates a strong dissolution tendency for halite and propensity for dissolution for gypsum, calcite, and dolomite. Additionally, pyrite oxidation and cation exchange processes occur from recharge to discharge zones.
Influence of Electric Field on Electrical Tree Growth in Epoxy Resin with Fillers
Authors: Htet Swe Zin Linn , Kondo Takuya , Miyake Takuma , Sakoda Tatsuya , Nishimura Takeshi 
Published: October 2023

Abstract
We investigate the influence of electric field in epoxy resin with fillers on electrical tree growth from a void. Not only observation of electrical trees but also measurements of temporal variations in partial discharge (PD) occurrence frequency and PD magnitude were carried out. It was found that the occurrence of PD with larger magnitude is less before breakdown and that there is a decreasing trend of the time required for breakdown with the increase of electric field in a void. Additionally, when electric field of the tip of a pit is almost the same as the withstand electric field of the epoxy resin, dielectric breakdown gradually occurs in a short time. However, the electrical tree growth is disturbed by fillers in epoxy resin, and the required time for breakdown of epoxy resin with fillers is much longer than that without fillers.
Flexoelectricity-driven toroidal polar topology in liquid-matter helielectrics
Authors: AYA Satoshi , Yang Jidan , Zou Yu , Li Jinxing , Huang Mingjun 
Published: October 2023

Abstract
Magnetic and electric dipoles share the symmetry similarity, being expected to exhibit many common structures. However, despite various spin textures composed of magnetic dipoles being reported frequently, investigations on long-range ordered electric dipoles have been scarcely made until recently. Here, we discover spontaneous toroidal polar topology in an emerging ferroelectric liquid state with polarisation helices, dubbed helielectric nematic. The interplay between the liquid crystal elasticity and polar interactions results in a continuous in-plane rotation of the polarisation, producing a periodic toroidal assembly. Partially in analogy with magnetism but different, the neighbouring toroidal domains are separated by circular-shaped domain walls. With an ultra-sensitive electric field down to 10-4 V μm-1, the local polarisation switching enables unique shrinkage/expansion dynamics of the toroidal domains. The discovery enriches polar topology and provides unprecedented opportunities to develop designable and switchable ferroelectric-liquid-matter optoelectronics.
Dynamics of Gasoline Price Changes; The Effects of Pricing Policies in Iran
Authors: Saryazdi Ali Haji Gholam , Tavangar Leila 
Published: October 2023

Abstract
The government as the determinant of gasoline prices in Iran has always faced challenges because of economical, social, cultural and environmental effect of gasoline price changes. In recent years, different implemented pricing policies have had varied consequences and, in some cases, have not necessarily achieved the purposes that the policy makers sought. Since the dynamics of the gasoline pricing system in IRAN's economy resulted in undesirable outcomes, this article aims to understand the structure governing gasoline pricing, price change policies, and their effects to compare different policies related to this subject and provide practical suggestions for gasoline pricing in IRAN. The system dynamics approach is used to illustrate the structure governing the price of gasoline and identification and simulation of different policies' effects on model behavior. The simulation results showed that if the politician's goal is to decrease the prices, the best policy is inflation-based pricing and price consolidation, although the gasoline demand (consumption and smuggling) is maximum. But if the goal is to decrease consumption, the ideal policy is pricing based on regional prices or supply and demand with gradual change.
Coupling design and fabrication of continuous carbon fiber reinforced composite structures using two-material topology optimization and additive manufacturing
Authors: Ho Thuan N.-T. , Nguyen Son H. , Thao Le Van , Hoang Tien-Dat 
Published: October 2023

Abstract
This study presents an integration of the level set-based two-material topology optimization method and the additive manufacturing technique for the design and fabrication of continuous carbon fiber (CCF)-reinforced composite structures. Firstly, optimal configurations of the resin material and fiber reinforcement are obtained to maximize the structural stiffness under desired volume constraints using the two-material topological optimization. After that, the level set-based cutting mesh method and triangulation scheme are employed to interpret these topological designs into stereolithography (STL) models with clear structural boundaries for the manufacturing. A customized pre-processing strategy is used to accurately determine the fiber placement regions from the optimal designs. Topological results are then fabricated using the CCF-based 3D printing method with prepreg carbon fibers. Subsequently, the performance of printed CCF-reinforced composite structures is investigated, using different resin materials: Esun polylactic acid (EPLA) and polyamide 12 with 10% carbon fiber (PA12\_10CF). Experimental results indicate a significant increase in stiffness and strength of composite structures with fiber reinforcements for all resin materials, with an increase of 315% for EPLA and 234% for PA12\_10CF. Additionally, the CCF-reinforced composite structures made of PA12\_10CF exhibit superior stiffness compared to those made of EPLA with a double increment. The microstructural characteristics of damaged regions are examined using scanning electron microscope (SEM) images, which provide valuable insights into the behavior of resin and fiber materials.
Gender Discrimination as an Ophthalmic Surgeon; A survey of Medical personnel at Eye Department of Mayo Hospital
Authors: Imtiaz Hafiza Sadia , Siddiqui Zahid kamal , Rehman Shujah Ur , Rafique Memoona , Sharjeel Muhammad 
Published: October 2023

Abstract
Objective: To determine gender preferences of medical personnel when selecting ophthalmologists for routine check-up and ophthalmic surgeries and also to determine the factors responsible for this gender-discrimination while selecting a surgeon.
Material and Methods: After getting ERB approval and taking informed consent from every participant, a cross-sectional study was conducted at Eye unit 3, Mayo Hospital, for a duration of 3 months using convenience sampling. 100 participants of either gender, of age 18 years or above, who are medical personnel working in Eye department of Mayo Hospital, including doctors and non- doctors’ staff were included in this study. Data was recorded on specially designed questionnaire and further analyzed on spss.
Results: 100 participants were enrolled in this study, out of which 62% were male and 38% were female. Mean age recorded was 32±2.4 years. Majority of participants didn’t have any preference (50.2%) in total and among them the highest level of no preference was for regular checkups (66%) and for Emergency cases (55%). While for elective surgical procedures, majority have male preference for vitreoretinal cases (50%) and for complicated cases (48%). While female surgeons were preferred compared to males only in pediatric ophthalmology cases (33% versus 21%). Among the positive characteristics of a female surgeon, mostly agreed that females are more detail oriented, have patient centered communication style, more empathetic, and understand the psychological factors better and are well-qualified. Mostly agreed that male surgeons are more confident, have strong decision power, better in dealing with complex cases and intra-operative complications and also performing in emergency situation.
Conclusion: In conclusion, almost half of participants didn’t show any preference and among remaining participants, male surgeons were preferred over female surgeons. Male surgeons were preferred for being more confident, fearless, more experienced, competent in dealing with complicated cases, more decisive, and having better professional skill.
Hypoxia upregulateing ACSS2 enhances lipid metabolism reprogramming through HMGCS1 mediated PI3K/AKT/mTOR pathway to promote the progression of pancreatic neuroendocrine neoplasms
Authors: Gu Danyang , Ye Mujie , Zhu Guoqin , Bai Jianan , Chen Jinhao , Yan Lijun , Yu Ping , Lu Feiyu , Hu Chunhua , Zong Yuan , Liu Pengfei , He Qibin , Tang Qiyun 
Published: October 2023

Abstract
Background
Pancreatic neuroendocrine neoplasms(pNENs) are a relatively rare disease.Hypoxia and lipid metabolism-related gene acetyl-CoA synthetase 2(ACSS2) is involved in tumor progression,but its role in pNENs is not revealed.This study showed that hypoxia can upregulate ACSS2,which plays an important role in the occurrence and development of pNENs through lipid metabolism reprogramming.However,the precise role and mechanisms of ACSS2 in pNENs remain unknown.
Methods
mRNA and protein levels of ACSS2 and 3hydroxy-3methylglutarylCoA synthase1(HMGCS1) were detected using quantitative real-time PCR(qRT-PCR) and Western blotting(WB).The effects of ACSS2 and HMGCS1 on cell proliferation were examined using CCK-8, colony formation assay and EdU assay, and their effects on cell migration and invasion were examined using transwell assay.The interaction between ACSS2 and HMGCS1 was verified by Co-immunoprecipitation(Co-IP) experiments,and the functions of ACSS2 and HMGCS1 in vivo were determined by nude mouse xenografts.
Results
We demonstrated that hypoxia can upregulate ACSS2 while hypoxia also promoted the progression of pNENs.ACSS2 were significantly upregulated in pNENs,overexpression of ACSS2 promoted the progression of pNENs and knockdown of ACSS2 and ACSS2 inhibitor(ACSS2i) trearment inhibited the progression of pNENs.ACSS2 regulated lipid reprogramming and the PI3K/AKT/mTOR pathway in pNENs,ACSS2 regulated lipid metabolism reprogramming through the PI3K/AKT/mTOR pathway.Co-IP experiments indicated that HMGCS1 interacted with ACSS2 in pNENs.Overexpression of HMGCS1 can reverse the enhanced lipid metabolism reprogramming and tumor-promoting effects of knockdown of ACSS2.Moreover,overexpression of HMGCS1 reversed the inhibitory effect of knockdown of ACSS2 on the PI3K/AKT/mTOR pathway.
Conclusion
Our study revealed that hypoxia can upregulate the lipid metabolism-related gene ACSS2,which plays a tumorigenic effect by regulating lipid metabolism through activating the PI3K/AKT/mTOR pathway.In addition,HMGCS1 can reverse the oncogenic effects of ACSS2, providing a new option for therapeutic strategy.
Unraveling the Role of Transcription Factor SP1 and H3K9 Acetylation in MGMT Regulation and Temozolomide Resistance in Glioblastoma Multiforme
Authors: Ye liguo , Gu Lingui , Wang Yaning , Xing Hao , Hu Ping , Wang Yu , Ma Wenbin 
Published: October 2023

Abstract
Glioblastoma multiforme (GBM) is recognized as a highly aggressive brain tumor, exhibiting profound resistance to temozolomide (TMZ) chemotherapy. While methylation of the MGMT promoter has been identified as a significant factor determining the response to TMZ, recent evidence indicates that the regulatory mechanisms are multifaceted, involving complex interplays with broader epigenetic and transcriptional landscapes. In this study, we meticulously developed TMZ-resistant GBM cell lines and applied a comprehensive suite of analytical methodologies, including transcriptomic and proteomic analyses, and single-cell transcriptomic profiling, to elucidate the molecular complexities associated with TMZ resistance. Our results demonstrate a crucial correlation between increased levels of H3K9 acetylation (H3K9ac), elevated MGMT expression, and heightened TMZ resistance, a correlation that remains steadfast even in cell lines with methylated MGMT promoters. Simultaneously, we identified the transcription factor SP1 as a key regulator, synergizing with H3K9ac to orchestrate MGMT transcription. Single-cell analysis revealed subtle expression discrepancies among GBM cell subpopulations, with a propensity for elevated H3K9ac pathway activity predominantly in the mesenchymal subtype. As tumors relapsed, we documented a transformation in the cellular character of GBM, migrating from a stem-like state to differentiation. This transition is marked by a progressive upregulation of MGMT, SP1, and H3K9ac. These findings suggest that while the clinical relevance of MGMT promoter methylation is undeniable, a comprehensive understanding of the resistance mechanisms in GBM necessitates a focus on the synergy between histone modifications, transcriptional regulations, and cellular diversity. By deconstructing these intricate relationships, this research enhances our comprehension of the inherent resilience of GBM to TMZ.
Nitrogen leaching and soil nutrient supply vary spatially within a temperate tree-based intercropping system but are unaffected by tree root pruning
Authors: Rivest David , Martin-Guay Marc-Olivier 
Published: October 2023

Abstract
Well-designed temperate tree-based intercropping (TBI) systems can enhance soil nutrient cycling compared to conventional agricultural systems. To improve the TBI designs and their subsequent wide-scale adoption, greater understanding is required regarding the extent to which widely-spaced tree rows and tree management practices influence spatio-temporal dynamics of soil nutrients. Our two-year study (2021 and 2022) assessed N-leaching and soil nutrient supply at increasing distances from tree rows (0, 4, 12, 20 m); the 10-year-old TBI system (50 trees ha− 1) together with agricultural controls was established in southern Québec (Canada). The TBI included hybrid poplars (Populus deltoides × P. nigra) planted alternately with high-value hardwoods in the rows. In each experimental block (n = 3), the TBI system and control were divided into two treatments: without root-pruning vs with (75 cm depth using a sub-soiler). In 2022, NO3− supply rates near tree rows (0 and 4 m) were lower than alley centres (12 and 20 m) and controls. A first structural equation modelling (SEM) analysis revealed that NO3− supply rates were mostly modulated by indirect effects of tree row distance and soil clay content through volumetric water content (VWC). NO3− leaching (40-cm depth) at 0 and 4 m from the tree row was repectively 8.8x and 7.5x lower than that in the control. A second SEM analysis showed direct and indirect (through soil VWC affecting NO3− supply rates) effects of distance from tree rows on NO3− leaching rates. Within TBI greater tree leaf litter dry-mass was trapped at 0 and 4 m vs 12 and 20 m. P and K availability under tree rows was higher than all other distances within cultivated alleys and control plots. P, K, Ca and Mg supplies within cultivated alleys werer generally similar among distances (4, 12, and 20 m) and did not differ from controls. An unexpected lack of effect of tree root pruning was observed regarding soil nutrient supply and N leaching. Clay content was a major driver of soil nutrient supply and N leaching. The role of TBI systems in determining soil nutrient dynamics depended upon the soil nutrient and sampling period that was measured, with greater effects beneath the trees and at the tree-crop interface.
Wastewater Treatment of Food Industry Enterprises Using Electrochemically Generated Ferrates
Authors: Kireev Sergey , Shtepa Vladimir , Kireeva Svetlana , Kozyr Alexey , Shikunets Alexey , Naumov Lev 
Published: October 2023

Abstract
The article considers a complex technology for electrochemical wastewater treatment from a meat processing plant. The process of water treatment includes a sequential processing in graphite and iron anode-based electrochemical modules, and exposure in maturation and homogenization chamber. Ferrate generation has been evidenced in the iron anode-based module, and ferrates concurrently acting as mild oxidants, coagulants, and steel corrosion inhibitors.
Quantitative Interpretation of Potential Field Data Covering Oml 29 and 11 Areas of Niger Delta, Nigeria
Authors: Ofoha C. C , Emujakporue Godwin , Omajene A 
Published: October 2023

Abstract
Hydrocarbon exploration potential of the Niger Delta, Nigeria, without doubt, contributes greatly to the economy of the nation due to its sedimentary thickness. However, the sedimentary thickness of the region has been documented but there is a paucity of information in the usage of the high-resolution aeromagnetic data to determine the sedimentary thickness of Oloibiri (OML 29) and Degema (OML 11) areas. Thus, this study was undertaken to determine the sedimentary thickness of the area using the high-resolution aeromagnetic data acquired from the Nigeria Geological Survey Agency (NGSA). Spectral depth analysis, Standard 3D Euler Deconvolution, and Source Parameter imagine (SPI) techniques were employed to estimate the sedimentary thickness of the area. A true depth of 8.41 km, 7.01 km, and 5.5 km using spectral depth analysis, Euler Deconvolution with a Structural Index (SI) of 1, and Source Parameter Imaging techniques, respectively, were revealed. These values fall within the sedimentary thickness range of the region as the data was employed. Thus, if other conditions are met, the area is plausible for hydrocarbon probing.
Development and validation of a predictive model for double-J stent encrustation after upper urinary tract calculi surgery
Authors: Jia Weihui , Chi Wenyu , Liu Chen , Song Yifen , Yang Shunshun , Yin Chonggao 
Published: October 2023

Abstract
The study is aimed to establish a predictive model of double-J stent encrustation after upper urinary tract calculi surgery. We collected the clinical data of 561 patients with indwelling double J tubes admitted to a hospital in Shandong Province from January 2019 to December 2020 as the modeling group and 241 cases of indwelling double J tubes from January 2021 to January 2022 as the verification group. Univariate and binary logistic regression analysis were used to explore risk factors, the risk prediction equation was established and the receiver operating characteristic (ROC) curve analysis model was used for prediction. In this study, 104 of the 561 patients developed double-J stent encrustation, with an incidence rate of 18.5%. We finally screened out BMI > 23.9 (OR = 1.648), Preoperative urine routine white blood cell quantification (OR = 1.149), double J tube insertion time (OR = 0.566), postoperative water consumption did not reach 2000ml/d (OR = 8.514) a total of 4 Factors build a risk prediction model. From the ROC curve analysis, the area under the curve (AUC) was 0.844, and the maximum Oden index was 0.579. At this time, the sensitivity was 0.735, and the specificity was 0.844. The research established in this study has a high predictive value for the occurrence of double-J stent encrustation in the double-J tube after upper urinary tract stone surgery, which provides a basis for the prevention and treatment of double-J stent encrustation.