bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaction of human herpesvirus 6 with human CD34 positive cells
Authors: Isomura Hiroki , Yoshida Mariko , Namba Hikaru , Yamada Masao 
Published: May 2003

AbstractWe reported previously that human herpesvirus 6 (HHV‐6) suppresses hematopoietic colony formation of erythroid (BFU‐E), granulocyte/macrophage (CFU‐GM), and megakaryocyte (CFU‐Meg) lineages in vitro. Here we describe the interaction between HHV‐6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti‐CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV‐6, two transcripts of the immediate early genes were detected with both cell populations. HHV‐6 suppressed colony formation of BFU‐E, CFU‐GM, and CFU‐Meg. HHV‐6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV‐6. These data indicate that the targets for hematopoietic suppression by HHV‐6 are the differentiated cells. J. Med. Virol. 70:444–450, 2003. © 2003 Wiley‐Liss, Inc.
Human herpesvirus‐6 modulates RANTES production in primary human endothelial cell cultures
Authors: Caruso Arnaldo , Favilli Flavia , Rotola Antonella , Comar Manola , Horejsh Douglas , Alessandri Giulio , Grassi Manuela , Di Luca Dario , Fiorentini Simona 
Published: May 2003

AbstractHuman herpesvirus 6 (HHV6) is a beta‐herpesvirus capable of infecting several cell types from different origins. HHV6 is the etiological agent of exantem subitum and has been associated with several diseases, all characterized by an inflammatory response triggered by chemokines. We show that strain U1102 of HHV6 is able to infect persistently human endothelial cells obtained from umbilical veins, adult aorta and adult heart microvessels, without apparent cytopathic effect. Analysis by in situ PCR showed that HHV6 sequences were present in 20% of HUVEC, 10% of aortic, and 1% of heart microvascular endothelial cells. Regardless of endothelial cell origin, HHV6 infection induced de novo synthesis of the RANTES CC‐chemokine. It was found, however, that microvascular endothelial cells, despite their lower susceptibility to HHV6 infection, showed the highest RANTES expression. Chemokine production occurred also in the absence of viral DNA synthesis. Furthermore, RANTES synthesis required an active viral genome, as UV‐inactivated HHV6 infection of endothelial cells did not lead to chemokine production. We investigated the expression of HHV6 U51 gene, which encodes a chemokine receptor that is already known to sequester and down modulate RANTES in epithelial cells. HHV6‐infected endothelial cells co‐expressed RANTES and U51 mRNAs starting from 12 hr up to 48 hr post‐infection. Then, RANTES transcripts disappeared whereas U51 messages continued to be expressed. In conclusion, this study highlights the major role of HHV6 in endothelial cell biology and the development of inflammatory processes. J. Med. Virol. 70:451–458, 2003. © 2003 Wiley‐Liss, Inc.
Virology of infantile chronic recurrent parotitis in Santiago de Chile
Authors: Vinagre Claudia , Martínez María José , Avendaño Luis Fidel , Landaeta Mirta , Pinto María Eugenia 
Published: May 2003

AbstractInfantile chronic recurrent parotitis (ICRP) has been attributed to multiple causes, including viral infections, and therefore its treatment remains empirical. Our aim was to evaluate the involvement of respiratory and oropharyngeal viruses in acute episodes of ICRP. Seventy children were studied, 50 patients and 20 age‐matched controls, in a 2‐year follow‐up study. Saliva samples were taken from the parotid duct and analyzed by viral isolation and immunofluorescence for adenovirus (Ad), respiratory sincitial virus (RSV), parainfluenza virus (PI), influenza virus (Flu), Cytomegalovirus (CMV), and herpes simplex virus (HSV). Paired sera samples were tested by ELISA for anti‐Epstein‐Barr virus (EBV) IgG and anti‐mumps IgM and IgG. Viral infections were detected in 7/50 (14%) cases of the ICRP group: one CMV; 2 Enteroviruses isolated in human embryonic lung fibroblast cells; 1 Flu A; and 3 mumps virus. No EBV seroconversions were detected. In the control group, 2 out of the 20 children had an asymptomatic mumps positive IgM titer. Our data indicate that the main respiratory and oropharyngeal viruses are not the cause of acute episodes of ICRP in Chilean children. J. Med. Virol. 70:459–462, 2003. © 2003 Wiley‐Liss, Inc.
Molecular characterization of influenza B viruses circulating in northern Italy during the 2001–2002 epidemic season
Authors: Ansaldi Filippo , D'Agaro Pierlanfranco , de Florentiis Daniela , Puzelli Simona , Lin Yi Pu , Gregory Victoria , Bennett Michael , Donatelli Isabella , Gasparini Roberto , Crovari Pietro , Hay Alan , Campello Cesare 
Published: May 2003

AbstractDuring the 2001–2002 influenza season, virological surveillance highlighted the predominant circulation of B viruses (86% of isolates) in Italy, in contrast to many other countries in Europe and North America where AH3N2 viruses were isolated most frequently, and in contrast to the infrequent isolation of B viruses in Italy during the previous two years. Associated with this predominance of influenza B was the re‐emergence of B/Victoria/2/87‐lineage viruses, closely related to B viruses prevalent during the 1980s, which are distinct antigenically and genetically from circulating B/Sichuan/379/99‐like viruses of the B/Yamagata/16/88 lineage, which predominated in most parts of the world during the last 10 years. Ninety‐four viruses isolated in two regions of northern Italy were characterized, 50 by direct sequencing of haemagglutinin (HA). Viruses of both Victoria and Yamagata lineages co‐circulated throughout the 12 weeks of the influenza season. The HAs of the Yamagata‐lineage viruses were heterogeneous and comprised two sublineages, represented by B/Sichuan/379/99 and B/Harbin/7/94, whereas the Victoria‐lineage viruses were more homogeneous and closely related to B/Hong Kong/330/01, the current prototype vaccine strain. The antigenic and genetic characteristics of the viruses correlated with certain epidemiological features. In particular, the low age (<14 years) of individuals infected with B/Hong Kong/330/01‐like viruses is likely to reflect the greater susceptibility of the youngest cohort, due to lack of previous exposure to Victoria‐lineage viruses, and is consistent with the conclusion that vaccination with a B/Sichuan/379/99‐like virus would give poor protection against infection with B/Hong Kong/330/01‐like (Victoria‐lineage) viruses. J. Med. Virol. 70:463–469, 2003. © 2003 Wiley‐Liss, Inc.
Manganese superoxide dismutase induction during measles virus infection
Authors: Wang Michael , Howell Joseph M. , Libbey Jane E. , Tainer John A. , Fujinami Robert S. 
Published: May 2003

AbstractMeasles virus infection of B‐cells results in marked alterations in proliferation and immunoglobulin production. Very little is known about the changes of gene expression, if any, during acute measles virus infection. To elucidate cellular genes that are induced during measles virus infection, we carried out a subtraction technique, representational differential analysis. The mitochondrial protein, manganese superoxide dismutase (MnSOD), was upregulated in B‐cells during measles virus infection. Although measles virus‐infected B‐cells did not secrete MnSOD into the environment, it was found, using an MnSOD mimetic, that intracellular MnSOD did inhibit proliferation of the B‐cells. MnSOD also decreases the titer of virus produced from infected cells. Therefore, MnSOD seems to play a role in the alteration of immune function seen upon infection of B‐cells with measles virus. J. Med. Virol. 70:470–474, 2003. © 2003 Wiley‐Liss, Inc.
Ex vivo cytokine responses against parvovirus B19 antigens in previously infected pregnant women
Authors: Corcoran Amanda , Mahon Bernard P. , McParland Peter , Davoren Anne , Doyle Sean 
Published: May 2003

AbstractParvovirus B19 infection is a significant cause of fetal death. The aim of this study was to investigate the role of maternal immune status in modulating susceptibility to fetal B19 infection. Peripheral blood was obtained from pregnant women (n = 199) with no clinical evidence of recent B19 infection. Evaluation of ex vivo T cell responses from 149/199 individuals showed significantly higher interferon‐γ levels for seropositive individuals following VP1 (268 ± 36 versus 103 ± 19 pg/ml; P = 0.003) and VP2 (242 ± 42 versus 91 ± 16 pg/ml; P = 0.01) antigen stimulation. Significantly higher levels of interleukin‐2 were also observed in seropositive individuals following both VP1 (P = 0.0003) and VP2 (P = 0.0005) stimulation. The observed Th1 cellular response is lower than that documented previously for non‐pregnant individuals and strongly suggests that diminution of the maternal anti‐viral immune response may increase susceptibility to fetal B19 infection. J. Med. Virol. 70:475–480, 2003. © 2003 Wiley‐Liss, Inc.
Detection of human respiratory syncytial virus sequences in peripheral blood mononuclear cells
Authors: Yui Ikuko , Hoshi Akiyoshi , Shigeta Yukiko , Takami Takeshi , Nakayama Tetsuo 
Published: May 2003

AbstractPeripheral blood mononuclear cells (PBMC) obtained from patients with lower respiratory infections were examined for the detection of human respiratory syncytial virus (RSV) sequences in the N region using the reverse transcription polymerase chain reaction (RT‐PCR). RSV infection was confirmed by at least one method, i.e., virus isolation, enzyme immunoassay for viral antigen, and RT‐PCR of nasopharyngeal secretions (NPS) samples. The detection rate for RSV RNA in PBMC obtained from RSV‐infected patients was 40% (38/94), compared to 5% (1/20) in controls (P = 0.002). Between the groups positive (38) and negative (56) for RSV RNA in PBMC, there was no significant difference in clinical parameters. Seven patients had eight episodes of reinfection and RSV RNA was detected in 50% (4/8) during consecutive infections. Sequences of their PBMC samples were distinct from those of prototype strains of subgroup A and B. However, they were not always consistent with those of paired NPS samples. The findings suggested that RSV RNA could be detected in PBMC even during reinfection and as might have the possibility of quasispecies dynamics, reflecting the nature of RNA viruses. J. Med. Virol. 70:481–489, 2003. © 2003 Wiley‐Liss, Inc.
Studies of neutralising antibodies to SV40 in human sera
Authors: Minor P. , Pipkin P. , Jarzebek Z. , Knowles W. 
Published: May 2003

AbstractIt has been suggested that the low levels of antibody to the simian polyoma virus SV40 found in human sera may be linked to the use of polio vaccines. Panels of sera from areas of the world with different vaccination histories were examined to see if consistent differences could be identified. In a total of 2,054 sera from the United Kingdom, 692 from Africa and 923 from Poland taken between 1985 and 1997, the seroprevalence was generally between 3 and 5%, although exceptionally one collection from Morocco had a prevalence of 100%, and one from Poland of 0.4%. The seroprevalence showed no obvious age‐dependent increase and titres were low compared to post infection animal sera. The results are consistent with previous studies and reveal no general geographically based differences related to possible differences in vaccination history, but the origin of the SV40 antibody in human sera remains to be established. J. Med. Virol. 70:490–495, 2003. © 2003 Wiley‐Liss, Inc.
Clinicopathological characteristics of superficial spreading type early gastric cancer
Authors: Imai Masato , Kondo Yukifumi , Osawa Shohei , Nishida Yasunori , Okada Kuniaki , Ishizu Hiroyuki , Masuko Hiroyuki , Hata Tsunetake , Uemura Kazuhito , Kina Masaya , Honda Shohei , Ishiyama Gentaro , Takahashi Toru , Hino Atsushi 
Published: May 2003

AbstractBackground and ObjectivesSuperficial spreading type early gastric cancer is characterized by its atypical growth pattern and occasionally indistinct tumor margin. Because it is a rare form of early gastric cancer, the clinicopathological details are not apparent. The aim of this study was to clarify the clinicopathological features of the superficial spreading type of early gastric cancer.MethodsA retrospective study was conducted in 1,062 surgically resected patients with early gastric cancer. Hospital records were compared between patients with superficial spreading type early gastric cancer and those with more common types of early gastric cancer.ResultsSixty‐nine patients (6.9%) had superficial spreading lesions. The male to female ratio was 1.2:1. The most frequent histological type was signet‐ring cell carcinoma (32%). The distinguishing histopathological features were submucosal invasion (67%), lymphatic invasion (32%), and lymph node metastasis (30%). There were discrepancies in tumor area between surgical findings and pathological diagnosis in 24 patients (35%) with superficial spreading type. More extensive lymph node dissection was performed and all patients survived in the group with superficial spreading lesions.ConclusionsThe most appropriate treatment for the superficial spreading type of early gastric cancer is wide surgical resection with extensive lymph node dissection. J. Surg. Oncol. 2003;83:94–98. © 2003 Wiley‐Liss, Inc.
Apolipoprotein D expression in cutaneous malignant melanoma
Authors: Miranda Eva , Vizoso Francisco , Martín Arancha , Quintela Isabel , Corte María Daniela , Seguí María Eugenia , Ordiz Iratxe , Merino Antonio Martínez 
Published: May 2003

AbstractBackground and ObjectivesApolipoprotein D (Apo D) is a protein component of the human plasma lipid transport system, and an androgen‐regulated protein in both breast and prostate cancer cell lines. Our goal was to evaluate the expression of Apo D in malignant cutaneous melanomas, as well as to assess its possible relationship to clinical and pathological parameters.MethodsApo D expression was analyzed in 32 paraffin‐embedded tissues from patients with invasive cutaneous malignant melanomas, in 8 samples from in situ melanoma, and in 10 samples from 10 benign lesions (4 dermal melanocytic nevi, 4 compound melanocytic nevi, and 2 dysplastic melanocytic nevi), using immunohistochemical techniques.ResultsThe benign lesions were consistently negative for Apo D, whereas 3 of the 8 “in situ” melanomas (37.5%) and 12 of the 32 invasive melanomas (37.5%) showed positive immunostaining for Apo D. The percentage of Apo D‐positive tumors was significantly higher in nodular than in superficial spreading melanomas (P = 0.011) and in melanomas with vertical growth phase than in melanomas with radial growth phase (P = 0.02). In addition, the percentage of Apo D‐positive tumors was positively and significantly correlated with Clark's level of invasion (P = 0.046).ConclusionsApo D may be a new prognostic factor of unfavorable evolution in cutaneous malignant melanoma. J. Surg. Oncol. 2003;83:99–105. © 2003 Wiley‐Liss, Inc.
Kinetic study of the gas‐phase reaction of the nitrate radical with methyl‐substituted thiophenes
Authors: Cabañas B. , Martín P. , Salgado S. , Baeza M. T. , Albaladejo J. , Martínez E. 
Published: May 2003

AbstractThe gas‐phase reactions of the NO3 radical with 2‐methylthiophene, 3‐methylthiophene, and 2,5‐dimethylthiophene have been studied, using relative and absolute methods at 298 K. Determination of relative rate was performed using Teflon collapsible bag as the reaction chamber and gas chromatography as the analytical tool. For the absolute method, experiments were carried out using fast‐flow‐discharge technique with detection of NO3 by laser‐induced fluorescence. The temperature dependence was studied by the absolute technique for the reactions of NO3 with 2‐methylthiophene and 3‐methylthiophene in the range 263–335 K.The proposed Arrhenius expressions for the reaction of the nitrate radical with 2‐methylthiophene and 3‐methylthiophene are k = (4 ± 2) × 10−16 exp[−(2200 ± 100)/T]] cm3 molecule−1 s−1 and k = (3 ± 2) × 10−15 exp[−(1700 ± 200)/T]] cm3 molecule−1 s−1, respectively. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 286–293, 2003
Experimental and modeling study of the oxidation of cyclohexene
Authors: Dayma G. , Glaude P. A. , Fournet R. , Battin‐Leclerc F. 
Published: May 2003

AbstractIgnition delays of cyclohexene–oxygen–argon mixtures were measured behind shock. Mixtures contained 1 or 2% of hydrocarbons for equivalence ratios ranging from 0.5 to 2. Reflected shock waves permitted to obtain temperatures from 1050 to 1520 K and pressures from 7.7 to 9.1 atm. The experimental results exhibit an Arrhenius variation vs. temperature.A detailed mechanism of the combustion of cyclohexene has been written in the line of the mechanism developed previously for the reaction of C3C4 unsaturated hydrocarbons (propyne, allene, 1,3‐butadiene, butynes); it is based on recent kinetic data values published in the literature and is consistent with thermochemistry. This mechanism has been validated by comparing the results of the simulations to the experimental results obtained for ignition delays. The main reaction pathways have been derived from flow rate and sensitivity analyses for the different temperature areas studied. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 273–285, 2003
Uncatalyzed and ruthenium(III)‐catalyzed reaction of acidic chlorite with methylene violet
Authors: Jonnalagadda S. B. , Shezi M. , Pare B. 
Published: May 2003

AbstractThe kinetics and mechanism of the uncatalyzed and Ru(III)‐catalyzed oxidation of methylene violet (3‐amino‐7‐diethylamino‐5‐phenyl phenazinium chloride) (MV+) by acidic chlorite is reported. With excess concentrations of other reactants, both uncatalyzed and catalyzed reactions had pseudo‐first‐order kinetics with respect to MV+. The uncatalyzed reaction had first‐order dependence on chlorite and H+ concentrations, but the catalyzed reaction had first‐order dependence on both chlorite and catalyst, and a fractional order with respect to [H+]. The rate coefficient of the uncatalyzed reaction is (5.72 ± 0.19) M−2 s−1, while the catalytic constant for the catalyzed reaction is (22.4 ± 0.3) × 103 M−1 s−1. The basic stoichiometric equation is as follows: 2MV+ + 7ClO2− + 2H+ = 2P + CH3COOH + 4ClO2 + 3Cl−, where P+ = 3‐amino‐7‐ethylamino‐5‐phenyl phenazinium‐10‐N‐oxide. Stoichiometry is dependent on the initial concentration of chlorite present. Consistent with the experimental results, pertinent mechanisms are proposed. The proposed 15‐step mechanism is simulated using literature; experimental and estimated rate coefficients and the simulated plots agreed well with the experimental curves. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 294–303, 2003
Rate coefficient for the reaction of CCl3 radicals with ozone
Authors: Imamura Takashi , Chono Hideharu , Shibuya Kazuhiko , Washida Nobuaki 
Published: May 2003

AbstractThe rate coefficient for the reaction of CCl3 radicals with ozone has been measured at 303 ± 2 K. The CCl3 radicals were generated by the pulsed laser photolysis of carbon tetrachloride at 193 nm. The time profile of CCl3 concentration was monitored with a photoionization mass spectrometer. Addition of the O3–O2 mixture to this system caused a decay of the CCl3 concentration because of the reactions of CCl3 + O3 → products (5) and CCl3 + O2 → products (4). The decay of signals from the CCl3 radical was measured in the presence and absence of ozone. In the absence of ozone, the O3–O2 mixture was passed through a heated quartz tube to convert the ozone to molecular oxygen. Since the rate coefficient for the reaction of CCl3 + O2 could be determined separately, the absolute rate coefficient for reaction (5) was obtained from the competition among these reactions. The rate coefficient determined for reaction (5) was (8.6 ± 0.5) × 10−13 cm3 molecule−1 s−1 and was also found to be independent of the total pressure (253–880 Pa of N2). This result shows that the reaction of CCl3 with O3 cannot compete with its reaction with O2 in the ozone layer. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 310–316, 2003
A shock tube study of the reaction NH2 + CH4 → NH3 + CH3 and comparison with transition state theory
Authors: Song Soonho , Golden David M. , Hanson Ronald K. , Bowman Craig T. , Senosiain Juan P. , Musgrave Charles B. , Friedrichs Gernot 
Published: May 2003

AbstractThe rate coefficient for NH2 + CH4 → NH3 + CH3 (R1) has been measured in a shock tube in the temperature range 1591–2084 K using FM spectroscopy to monitor NH2 radicals. The measurements are combined with a calculation of the potential energy surface and canonical transition state theory with WKB tunneling to obtain an expression for k1 = 1.47 × 103 T 3.01 e−5001/T(K) cm3 mol−1 s−1 that describes available data in the temperature range 300 –2100 K. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 304–309, 2003
New technique for generating high concentrations of gaseous OH radicals in relative rate measurements
Authors: Chen L. , Kutsuna S. , Tokuhashi K. , Sekiya A. 
Published: May 2003

AbstractWe have developed a technique for generating high concentrations of gaseous OH radicals in a reaction chamber. The technique, which involves the UV photolysis of O3 in the presence of water vapor, was used in combination with the relative rate method to obtain rate constants for reactions of OH radicals with selected species. A key improvement of the technique is that an O3/O2 (3%) gas mixture is continuously introduced into the reaction chamber, during the UV irradiation period. An important feature is that a high concentration of OH radicals [(0.53–1.2) × 1011 radicals cm−3] can be produced during the irradiation in continuous, steady‐state experiment. Using the new technique in conjunction with the relative rate method, we obtained the rate constant for the reaction of CHF3 (HFC‐23) with OH radicals, k1. We obtained k1(298 K) = (3.32 ± 0.20) × 10−16 and determined the temperature dependence of k1 to be (0.48 ± 0.13) × 10−12 exp[−(2180 ± 100)/T] cm3 molecule−1 s−1 at 253–328 K using CHF2CF3 (HFC‐125) and CHF2Cl (HCFC‐22) as reference compounds in CHF3–reference–H2O gas mixtures. The value of k1 obtained in this study is in agreement with previous measurements of k1. This result confirms that our technique for generating OH radicals is suitable for obtaining OH radical reaction rate constants of ∼10−16 cm3 molecule−1 s−1, provided the rate constants do not depend on pressure. In addition, it also needed to examine whether the reactions of sample and reference compound with O3 interfere the measurement when selecting this technique. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 317–325, 2003