bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of rare plasmid hosts using a targeted Hi-C approach
Authors: Castaneda-Barba Salvador , Ridenhour Benjamin J. , Top Eva M. , Stalder Thibault 
Published: December 2023

AbstractDespite the significant role plasmids play in microbial evolution, there is limited knowledge of their ecology, evolution, and transfer in microbial communities. Therefore, we developed and implemented a novel approach to identify rare plasmid hosts by combining Hi-C, a proximity ligation method, with enrichment for plasmid-specific DNA. We hereafter refer to this Hi-C enrichment approach as Hi-C+. Our experimental design mimicked scenarios in which the transfer of an antimicrobial resistance plasmid from a donor to a recipient in soil was increasingly rare. We established that Hi-C can link a plasmid to its host in soil when the relative abundance of that plasmid-host pair is as low as 0.001%. The Hi-C+ method further improved the detection limit of Hi-C 100-fold and allowed identification of plasmid hosts at the genus level. Therefore, Hi-C+ will facilitate the exploration of the ecological and evolutionary pathways that affect the spread of plasmids in natural environments.TeaserIn this study we demonstrate that a target-enriched Hi-C approach can identify rare hosts of a given plasmid in soil.
Up-regulation of Retrograde Response in yeast increases glycerol and reduces ethanol during wine fermentation
Authors: Garrigós Víctor , Vallejo Beatriz , Mollà-Martí Esperanza , Picazo Cecilia , Peltier Emilien , Marullo Philippe , Matallana Emilia , Aranda Agustín 
Published: December 2023

AbstractNutrient signaling pathways play a pivotal role in regulating the balance between metabolism, growth and stress response depending on the available food supply. They are key factor for the biotechnological success of the yeastSaccharomyces cerevisiaeduring food-producing fermentations. One such pathway is the Retrograde Response, which controls the use of carbon backbones for the synthesis of amino acids derived from alpha-ketoglutarate, such as glutamate and lysine. The repressorMKS1is linked to the TORC1 complex and negatively regulates this pathway. Deletion ofMKS1on a variety of industrial strains causes an increase in glycerol in winemaking, brewing and baking conditions. This increase is accompanied by a reduction in ethanol production in grape juice fermentations in four commercial wine strains. Interestingly, this does not lead to an increase in volatile acidity, as the levels of acetic acid actually decrease. Aeration during winemaking usually increases acetic acid levels, but this effect is reduced in theMKS1mutant. Although there is an improvement in the metabolites of oenological interest, it comes at a cost, as the mutant showed slower fermentation kinetics when grown in grape juice, malt, and laboratory media using glucose, sucrose, and maltose as carbon sources. The deletion ofRTG2, an activator of the Retrograde Response that acts as an antagonist ofMKS1, also results in a defect in wine fermentation speed. These findings suggest that the deregulation of this pathway causes a fitness defect. Therefore, manipulating the repressorMKS1is a promising approach to modulate yeast metabolism and produce low-ethanol drinks.
Protein tyrosine phosphatase receptor kappa regulates glycolysis andde novolipogenesis to promote hepatocyte metabolic reprogramming in obesity
Authors: Gilglioni Eduardo H. , Li Ao , St-Pierre-Wijckmans Wadsen , Shen Tzu-Keng , Pérez-Chávez Israel , Hovhannisyan Garnik , Lisjak Michela , Negueruela Javier , Vandenbempt Valerie , Bauzá-Martinez Julia , Herranz Jose M. , Ezerina Daria , Demine Stephane , Feng Zheng , Vignane Thibaut , Otero-Sánchez Lukas , Lambertucci Flavia , Prašnická Alena , Devière Jacques , Hay David C. , Encinar Jose A. , Singh Sumeet Pal , Messens Joris , Filipovic Milos R. , Sharpe Hayley J. , Trépo Eric , Wu Wei , Gurzov Esteban N. 
Published: December 2023

AbstractFat accumulation,de novolipogenesis, and glycolysis are key drivers of hepatocyte reprogramming and the consequent metabolic dysfunction-associated steatotic liver disease (MASLD). Here we report that obesity leads to dysregulated expression of hepatic protein-tyrosine phosphatases (PTPs). PTPRK was found to be increased in steatotic hepatocytes in both humans and mice, and positively correlated with PPARγ-induced lipogenic signalling. High-fat-fed PTPRK knockout mice displayed reduced weight gain and hepatic fat accumulation. Phosphoproteomic analysis in primary hepatocytes and hepatic metabolomics identified fructose-1,6-bisphosphatase 1 and glycolysis as PTPRK targets in metabolic reprogramming. Silencing PTPRK in hepatoma cell lines resulted in reduced colony-forming ability and PTPRK knockout mice developed smaller tumours after diethylnitrosamine-induced hepatocarcinogenesis. Our study defines a novel role for PTPRK in regulating hepatic glycolysis, lipid metabolism, and tumour development. PTPRK inhibition may provide therapeutic possibilities in obesity-associated liver diseases.HighlightsHepatic receptor-type PTPs are increased in MASLDPTPRK is expressed in hepatocytes and upregulated in obesityPTPRK deficiency reduces body fat mass and liver steatosis in diet-induced obesityPTPRK regulates hepatic glycolysis and lipogenesis, promoting tumorigenesis
The S-O-R paradigm in explaining enterprise social network (ESN) discontinuous usage intention
Authors: Olfat Mohammad 
Published: December 2023

PurposeThis study aims to show that employees' excessive work-related use of enterprise social networks (ESN) can be accompanied by some work-related strains, hindering them from continuing utilization of ESN at work. To this end, the impact of employees' excessive work-related utilization of ESN on their discontinuous usage intentions by mediating roles of employees' impression management concerns, privacy concerns and ESN fatigue will be evaluated.Design/methodology/approachStimulus-organisms-response (S-O-R) framework has been drawn to support the design of this research. Using an entirely random data collection, 173 ESN users from 10 Iranian organizations were surveyed. The model was assessed using partial least squares structural equations modeling (PLS-SEM).FindingsThe results of the study confirm that employees' excessive work-related use of ESN positively affects impression management and privacy concerns, resulting in ESN fatigue. Furthermore, ESN fatigue plays a predicting role in ESN discontinuous usage intention.Originality/valueAccording to the obtained results, if work-related use of ESN exceeds a normal threshold (i.e. excessive usage), employees will stop using ESN in their work due to the work-related strains delivered to them, revealing the dark side of ESN usage in organizations.
The effect of the COVID-19 pandemic on Malaysian residential customers’ energy-saving appliance purchasing behaviour
Authors: Jaaffar Amar Hisham , Kasavan Saraswathy , Mustapa Siti Indati , Al-Amin Abul Quasem 
Published: December 2023

Purpose
The COVID-19 pandemic has caused a dramatic impact on energy supply and demand. It is vital to understand households’ behaviour with regard to energy, particularly during the pandemic, to deploy future sustainable energy systems. This study aims to investigate the nexus of Malaysian households’ energy consumption behaviour in relation to various electrical appliances, their energy-saving appliance purchasing behaviour and their current possession of energy-saving appliances during the pandemic, especially during the lockdown period, from the perspective of the energy cultures framework.

Design/methodology/approach
The partial least squares structural equation modelling technique was used to test hypothesised relationships based on the 1,485 pieces of household data collected using an online and physical survey during the lockdown period in Malaysia.

Findings
The energy-saving behaviour cultivated due to the impact of the COVID-19 pandemic led to residential customers’ intentions to purchase energy-saving appliances which subsequently led to their current possession of energy-saving appliances. Indeed, energy-saving behaviours in the kitchen, entertainment, office, home lighting and cooling appliances have more than 77.4% influence on their purchasing behaviour. The consumer’s purchase behaviour for energy-saving appliances has a significant, partially mediating influence on the energy-saving behaviour of various electrical appliances and the consumers’ current possession of energy-saving appliances.

Research limitations/implications
This study could be enhanced by improving the sample using a higher-income group and involving other parts of Malaysia such as the southern region. The findings do extend the energy cultures framework by demonstrating the mediating role of households’ energy-saving appliance purchasing behaviour on the relationship between their energy consumption behaviour in relation to various electrical appliances and their current possession of energy-saving appliances.

Practical implications
The results of this study will help develop future action plans for transitioning to energy-saving appliance practices.

Originality/value
This paper examines the effects of the COVID-19 pandemic on future energy efficiency practices in developing countries from the perspective of the energy cultures framework.
State of the art and future scenarios for bio-packaging market transition: evidence from Poland
Authors: Ocicka Barbara , Kędzia Grażyna , Brzeziński Jakub 
Published: December 2023

PurposeThe purpose of this article is twofold. First, this study characterises the current state of the bio-packaging market's development. Second, it identifies key factors influencing and possible scenarios of the bio-packaging market transition to increase the market share of compostable packaging.Design/methodology/approachThe results of 29 in-depth interviews (IDIs) with representatives of the key groups of bio-packaging supply chains' (SCs') stakeholders were the input for the consideration of the research problem.FindingsThe main economic, legal, social and technological enablers and barriers to the bio-packaging regime transition are recognised, and their impact at the market level is explained. The authors recognised the hybrid transition scenario towards an increase in the market share of compostable packaging related to the three traditional pathways of transformation, reconfiguration and technological substitution.Originality/valueThis study contributes to a better understanding of the socio-technical system theory by examining interdependencies between landscape (external environment), market regime (bio-packaging market) and niche innovations (compostable packaging) as well as system transition pathways. The findings and conclusions on bio-packaging market developments can be important lessons learnt to be applied in different countries due to the same current development stage of the compostable packaging lifecycle worldwide.
Does supply chain concentration improve sustainability performance: the role of operational slack and information transparency
Authors: Jia Fu , Xu Ying , Chen Lujie , Fernandes Kiran 
Published: December 2023

PurposeDespite the increasing interest in the role of supply chain concentration (SCC) in improving performance, its influence on firms' sustainability performance remains unexplored, as do the underlying mechanisms of this relationship. Drawing on resource dependence theory, the authors investigate the relationship between SCC and manufacturing firms' sustainability performance and the moderating roles of operational slack and information transparency.Design/methodology/approachThe authors use secondary data from 3,581 manufacturing firms listed on the Shanghai and Shenzhen A-share stock markets from 2006 to 2020 to conduct an empirical analysis using panel data regression models.FindingsManufacturing firms' SCC is negatively related to sustainability performance until it reaches a certain point, where SCC positively affects sustainability performance, presenting a U-shaped relationship. In addition, operational slack represented by a quick ratio moderates the relationship between SCC and sustainability performance by flattening the curve. Operational slack represented by receivable turnover ratio moderates the relationship between SCC and sustainability performance by steepening the curve and shifting the turning point left. Information transparency strengthens the effect of SCC on the sustainability performance by steepening the curve.Originality/valueThis investigation provides a comprehensive view of the SCC– sustainability performance relationship.
Understanding the effects of specific techno-stressors on strain and job performance: a meta-analysis of the empirical evidence
Authors: Yuan Qin , Kong Jun , Liu Chun , Jiang Yushi 
Published: December 2023

PurposeWhile the phenomenon of technostress has received significant attention from researchers in recent years, empirical findings concerning the consequences of specific forms of techno-stressors have remained scattered and contradictory. The authors aim to integrate the conclusions of previous studies to understand the effects of specific techno-stressors on strain and job performance.Design/methodology/approachThis study employs meta-analytic techniques to calibrate the findings of 67 studies investigating more than 63,100 employees.FindingsIn general, not all techno-stressors have adverse effects. In particular, techno-uncertainty does not impact job performance. In addition, relative weight analyses reveal the relative importance of techno-complexity and techno-insecurity as predictors of both strain and job performance. Finally, this study finds that the effects of specific techno-stressors on job performance vary depending on research participants' gender, educational attainment and employment status.Originality/valueFirst, this study provides a more nuanced view of the effects of specific techno-stressors. Second, this research clarifies the relative importance of specific techno-stressors as predictors of strain and job performance. Finally, this study reveals the moderating effects of demographic variables on the relationships between specific techno-stressors and job performance.
Can gamification affect the advertising effectiveness in social media?
Authors: Tabaeeian Reihaneh Alsadat , Rahgozar Shakiba , Khoshfetrat Atefeh , Saedpanah Samira 
Published: December 2023

PurposeThe purpose of this paper is to investigate how gamification in advertising affects the effectiveness of advertising on social media.Design/methodology/approachDrawing from the gamification literature, immersion, achievement and social interaction features were identified as features, and advertising effectiveness factors were brand attention, brand liking and recall. A questionnaire was used for collecting data, and a structural equation modeling approach was applied to examine the model.FindingsThe results indicated that immersion, achievement and social interaction features affect advertising effectiveness. Furthermore, advertising effectiveness factors had a significant effect on purchase intentions.Originality/valueThe study contributes to the combination of gamification in advertising by offering characteristics for gamification that can enhance advertising effectiveness through brand attention, liking and recall. Finally, it provides managerial guidance on how they can use gamification in their advertising process.
Investigating ground-related design deficiencies as potential triggers to cost overruns in highway projects
Authors: Amadi Alolote Ibim 
Published: December 2023

Purpose
This study aims to investigate ground-related design deficiencies as potential avenues of avoidable cost overruns, discernible from the geotechnical practices of highway agencies in the Niger Delta region of Nigeria.

Design/methodology/approach
The study deploys an interpretivist qualitative methodology to provide a detailed descriptive analysis of the design-related geotechnical practices of highway agencies during the pre-contract phase of highway projects. Semi-structured interviews were conducted with in-house professionals, consultants and contractors affiliated with the three highway agencies in the Niger Delta and thematically analysed to identify significant deviations from geotechnical best practices.

Findings
The study outcome shows that during the pre-contract phase, a chain of design-related geotechnical shortcomings has plagued highway projects executed in the Niger Delta. This view of practice uncovered in this study demonstrates a culture of significant deviation from best practice recommendations, which could plausibly contribute to the history of significant project cost overruns recorded in the region.

Originality/value
The study qualitatively spotlights gaps in the practice of highway agencies and reinforces the need for a re-orientation of the attitude to risk management, to give geotechnical concerns a priority in the financial management of highway projects executed in the Niger Delta region of Nigeria.
Do Instagram reels influence travelers' behavioral and e-WOM intentions for the selection of ecotourism destination?
Authors: Sharma Nidhi , Arora Nilesh 
Published: December 2023

PurposeThere has been considerable discussion about the utilization of social media effectively in tourist research. Still, there is a paucity of information about its usage for ecotourism destination selection. The study aims to determine critical factors influencing travelers' behavioral and electronic word-of-mouth (e-WOM) intentions to use Instagram reels to select an ecotourism destination.Design/methodology/approachThis study is based on the motivated consumer innovativeness theory and technology acceptance model. Purposive sampling was applied to acquire data from Instagram users. Data were gathered from 445 respondents and 415 valid responses were analyzed using partial least square structural equational modeling.FindingsThe findings of the study confirmed the positive impact of perceived ease of use, socially motivated consumer innovativeness and hedonically motivated consumer innovativeness on travelers' attitude except for perceived usefulness. The outcomes also revealed a significant influence of travelers' attitude on behavioral and e-WOM intention.Research limitations/implicationsThe study's findings were intended to offer insights into traveler behavior to critical players in the tourism sector, including destination marketers, travel companies, the government and policymakers. They must comprehend how useful Instagram is for the tourist industry, which will help them better understand how to attract travelers through Instagram reels to market their destination.Originality/valueThe current investigation is the first attempt to investigate the travelers' behavioral and e-WOM intentions to use Instagram reels to select an ecotourism destination.
Should I stay or should I go back? Factors influencing snowbirds' permanent relocation intention
Authors: Chaulagain Suja , Li Jianwen , Baniya Rojan , Pizam Abraham 
Published: December 2023

PurposeThe objective of the present study was to investigate the impacts of familiarity with and awareness of Florida as a snowbird destination, and satisfaction with the snowbird experience on Florida's image and on snowbirds' permanent relocation intention to Florida. Additionally, the influence of Florida's image on snowbirds' behavioral intention to move to Florida permanently was investigated.Design/methodology/approachData gathered from 304 snowbirds were utilized in employing structural equation modeling to assess the research model.FindingsThe study results showed that awareness of and familiarity with Florida as a snowbird destination, and satisfaction with the snowbird experience significantly influenced participants' image perceptions of Florida. In addition, awareness of and familiarity with Florida as a snowbird destination, and participants' image of Florida had a significant influence on snowbirds' intentions to move to Florida permanently.Practical implicationsThe findings of the study provide significant insights for destination marketing and management organizations and local policymakers in formulating and enacting policies in snowbird destinations to efficiently address migration trends.Originality/valueThis study is one of the first papers to empirically investigate and successfully construct a comprehensive model that explicate the determinants of snowbird tourists' permanent relocation intention.
Health care model for people living in nursing homes based on integrated care
Authors: Espaulella-Ferrer Mariona , Morel-Corona Felix Jorge , Zarco-Martinez Mireia , Marty-Perez Alba , Sola-Palacios Raquel , Campollo-Duquela Maria Eugenia , Cruz-Grullon Maricelis , Puigoriol-Juvanteny Emma , Otero-Viñas Marta , Espaulella-Panicot Joan 
Published: December 2023

PurposeOlder people living in nursing homes have complex care needs and frequently need specialists’ advice and support that can be challenging to deliver in a rural setting. The aim of this paper is to describe a model of integrated care in a rural area supported by a nurse case manager.Design/methodology/approachA real-world evidence study of people living in Ribes de Freser nursing home, was conducted between specific timeframes in 2019 and 2022, comparing the casemix and outcomes of a traditional care model with the integrated interdisciplinary model.FindingsThe integrated care model led to a significant reduction in transfers to the emergency department, hospitalisations, outpatient medical visits and a reduction in the number of medicines. In addition, the number of residents receiving end-of-life care at the nursing home showed a substantial increase.Originality/valueThis case study contributes valuable evidence supporting the implementation of an integrated model of nurse case manager support in nursing homes, particularly in the rural contexts, where access to specialist medical staff may be limited. The findings highlight the potential benefits of person-centred integrated care for older adults, addressing their complex needs and improving end-of-life care in nursing home settings.
The effects of low-carbon awareness on remanufacturing strategy in the presence of competition
Authors: Zheng Yanyan , Liu Peng , Zhao Yingxue , Zhang Zhichao 
Published: December 2023

PurposeThis paper examines how the level of low-carbon awareness (LCA) affects the remanufacturing strategy in a supply chain with an original equipment manufacturer (OEM) and an independent remanufacturer (IR) competing with each other.Design/methodology/approachGame theory and operations optimization.FindingsThe studies analytically characterize the threshold levels of the LCA in response to which the OEM and the IR will change their remanufacturing strategies from no remanufacturing to partial remanufacturing and then to full remanufacturing. In addition, the studies reveal that as compared with the OEM, the IR has more flexibility in terms of the market entry to remanufacturing with the level of LCA increasing. With the extended studies, it is exhibited that the above findings are robust to a good extent.Originality/valueIt can provide decision support for remanufacturing enterprises.