bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ignition Delay Times and Chemical Kinetic Model Validation for Hydrogen and Ammonia Blending With Natural Gas at Gas Turbine Relevant Conditions
Authors: Pierro Michael , Urso Justin J. , Rahman Ramees K. , Dennis Christopher W. , Albright Marley A. , McGaunn Jonathan , Kinney Cory , Vasu Subith S. 
Published: October 2023

Abstract
Ignition delay times from undiluted mixtures of natural gas (NG)/H2/Air and NG/NH3/Air were measured using a high-pressure shock tube at the University of Central Florida. The combustion temperatures were experimentally tested between 1000 and 1500 K near a constant pressure of 25 bar. As mentioned, mixtures were kept undiluted to replicate the same chemistry pathways seen in gas turbine combustion chambers. Recorded combustion pressures exceeded 200 bar due to the large energy release, hence why these were performed at the high-pressure shock tube facility. The data are compared to the predictions of the NUIGMech 1.1 mechanism for chemical kinetic model validation and refinement. An exceptional agreement was shown for stoichiometric conditions in all cases but strayed at lean and rich equivalence ratios, especially in the lower temperature regime of H2 addition and all temperature ranges of the baseline NG mixture. Hydrogen addition also decreased ignition delay times by nearly 90%, while NH3 fuel addition made no noticeable difference in ignition time. NG/NH3 exhibited similar chemistry to pure NG under the same conditions, which is shown in a sensitivity analysis. The reaction CH3 + O2 = CH3O + O is identified and suggested as a possible modification target to improve model performance. Increasing the robustness of chemical kinetic models via experimental validation will directly aid in designing next-generation combustion chambers for use in gas turbines, which in turn will greatly lower global emissions and reduce greenhouse effects.
Computed Tomography Artifact Reduction Employing a Convolutional Neural Network Within the Context of Dimensional Metrology
Authors: Ghafarzadeh Mahdi , Kejani Mohammad Tavakoli , Karimi Mehdi , Asadi Amirreza 
Published: October 2023

Abstract
Utilizing accurate, nondestructive testing methods to improve quality control and reduce manufacturing errors has gained prominence in light of industry development in various fields. Industrial computed tomography (CT) scanning carries considerable weight among all conventional methods because of their unique features, such as providing a three-dimensional specimen model. Due to the prevalence of metals with high linear attenuation coefficients in industrial applications, beam hardening and scatter artifacts are two of the most prevalent artifacts in any reconstructed volume. Other notable artifacts include those with a nonideal focal spot and conical beam radiation. These artifacts may manifest as a distortion of gray value peaks, systematic discrepancies, blurring-like cupping, and streaking in reconstructed images, degrading volume reconstruction quality. In this paper, the effect of these artifacts is illustrated and mitigated by adopting our proposed method, a combination of conventional and contemporary techniques, including the use of a pretrained convolutional neural network (CNN). Five tests are replicated in different geometric parameters to perform a geometric configuration analysis, indicating how effective the proposed approach is at encountering different geometric situations. The results demonstrate that the proposed method has substantially achieved its goal of improving the accuracy of dimensional metrology performed on our phantom.
Design and Dynamic Analysis of a Co-Existence Solution Between a Semi-Submersible Offshore Fish Cage and a Spar-Type Floating Offshore Wind Turbine
Authors: Ma Yu , Li Lin , Ong Muk Chen , Jin Jingzhe , Su Biao 
Published: October 2023

Abstract
Clean energy captured by offshore wind turbines has been widely used for supporting onshore activities. In the near future, facilities such as offshore wind turbines can also play an important role in the energy transition of offshore activities. Offshore wind energy can be employed for electrifying operations in offshore fish farms, which are traditionally supplied by diesel-engine barges/generators. Based on this motivation, this study focuses on the design of a shared mooring system between a semi-submersible offshore fish cage and a spar-type floating wind turbine. A numerical model of the proposed shared mooring system is implemented in a global response analysis software sima for performing fully coupled time-domain simulations. The configuration of the shared mooring line is determined using an engineering approach, which comprises Irvine’s formulation, system eigenvalue analysis, and cost estimation. Moreover, relevant case studies by altering the environmental conditions are performed. Extreme operational conditions that may give large relative motions are investigated thoroughly. The dynamic performance of the integrated system is compared with that of individual structures. The global motion of the floating wind turbine and its mooring line’s tension behavior are obviously influenced by the existence of the shared line. In general, the present work investigates the feasibility of a shared mooring system for these types of offshore structures and further gives insights into the engineering design procedure.
Effect of Friction Parameters on Tribological Properties of Erucamide as Grease Additive on GCr15 Steel
Authors: Liu Qingchun , Mo Yimin , Zhang Delai 
Published: October 2023

Abstract
The present study investigates the effect of friction parameters on tribological properties of erucamide as an additive in grease on GCr15 steel, as well as the sensitivity of erucamide to these parameters. An orthogonal test design is utilized to sample the friction parameters, followed by the construction of a Kriging model to articulate the relationship between these parameters and the designated objectives (average friction coefficient and wear volume). The Non-dominated Sorting Genetic Algorithm II (NSGA-II) is used to optimize the design objectives and verify the accuracy of the model. The Monte Carlo method is used to analyze the sensitivity of erucamide to the friction parameters. The results showed that erucamide exhibits excellent tribological properties at 80 N/20 Hz/25 °C and 20 N/20 Hz/25 °C. The sensitivity of erucamide to friction parameters from high to low is temperature, load, and speed. This can be ascribed to erucamide's unique structure, encompassing a globular aggregation head and a hydrophobic tail extending outward. The anti-friction effects and lubrication properties of erucamide are driven by its rheological properties and intermolecular interactions. At elevated temperatures, erucamide's fluidity escalates, favoring the lubricating film formation; however, excessive fluidity may impede its adherence to requisite locations, thereby resulting in compromised tribological properties of erucamide at high temperatures.
The Role of Frequency and Impedance Contrasts in Bandgap Closing and Formation Patterns of Axially-Vibrating Phononic Crystals
Authors: Al Ba’ba’a Hasan B. , Nouh Mostafa 
Published: October 2023

Abstract
Bandgaps, or frequency ranges of forbidden wave propagation, are a hallmark of phononic crystals (PnCs). Unlike their lattice counterparts, PnCs taking the form of continuous structures exhibit an infinite number of bandgaps of varying location, bandwidth, and distribution along the frequency spectrum. While these bandgaps are commonly predicted from benchmark tools such as the Bloch-wave theory, the conditions that dictate the patterns associated with bandgap symmetry, attenuation, or even closing in multi-bandgap PnCs remain an enigma. In this work, we establish these patterns in one-dimensional rods undergoing longitudinal motion via a canonical transfer-matrix-based approach. In doing so, we connect the conditions governing bandgap formation and closing to their physical origins in the context of the Bragg condition (for infinite media) and natural resonances (for finite counterparts). The developed framework uniquely characterizes individual bandgaps within a larger dispersion spectrum regardless of their parity (i.e., odd versus even bandgaps) or location (low versus high-frequency), by exploiting dimensionless constants of the PnC unit cell which quantify the different contrasts between its constitutive layers. These developments are detailed for a bi-layered PnC and then generalized for a PnC of any number of layers by increasing the model complexity. We envision this mathematical development to be a future standard for the realization of hierarchically structured PnCs with prescribed and finely tailored bandgap profiles.
A novel recombinant S-based subunit vaccine induces protective immunity against porcine deltacoronavirus challenge in piglets
Authors: Li Jizong , Zhao Shuqing , Zhang Baotai , Huang Jin , Peng Qi , Xiao Li , Yuan Xuesong , Guo Rongli , Zhou Jinzhu , Fan Baochao , Xue Tao , Zhu Xuejiao , Liu Chuanmin , Zhu Xing , Ren Lili , Li Bin 
Published: October 2023

ABSTRACT
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, causes diarrhea in piglets and possesses the potential to infect humans. However, there are no commercially available vaccines for PDCoV. In this study, the immune responses to the spike (S) protein and receptor-binding domain (RBD) trimer were examined in mice. Neutralization assays and flow cytometry analysis demonstrated that S protein elicited more robust neutralizing antibodies (NAbs) and cellular immune responses than the RBD trimer. Spike protein and inactivated vaccine were used in the assessment of immunogenicity in piglets and sows. Immunized piglets, sows, and suckling pig showed high NAb titers and S-specific sIgA in colostrum, milk, and serum. The piglets/suckling pig from the immunized group displayed significantly fewer microscopic lesions in the intestinal tissue, with only one or no piglet showing mild diarrhea. However, all piglets/suckling pig showed mild to watery diarrhea and exhibited a high level of viral shedding in the challenged control group. The feces from the piglets/suckling pig in the S protein and inactivated vaccine group exhibited reduced viral load. Of note, vaccine-elicited NAbs last for more than 4 months in immunized piglets. Together, our data demonstrate for the first time the protective efficacy of S-based subunit vaccine, which could be a candidate vaccine against PDCoV.

IMPORTANCE
As an emerging porcine enteropathogenic coronavirus that has the potential to infect humans, porcine deltacoronavirus (PDCoV) is receiving increasing attention. However, no effective commercially available vaccines against this virus are available. In this work, we designed a spike (S) protein and receptor-binding domain (RBD) trimer as a candidate PDCoV subunit vaccine. We demonstrated that S protein induced more robust humoral and cellular immune responses than the RBD trimer in mice. Furthermore, the protective efficacy of the S protein was compared with that of inactivated PDCoV vaccines in piglets and sows. Of note, the immunized piglets and suckling pig showed a high level of NAbs and were associated with reduced virus shedding and mild diarrhea, and the high level of NAbs was maintained for at least 4 months. Importantly, we demonstrated that S protein-based subunit vaccines conferred significant protection against PDCoV infection.
Comparison of
Helicobacter pylori
positive and negative gastric cancer via multi-omics analysis
Authors: Shang Fumei , Cao Yinghao , Wan Lixin , Ren Zhonghai , Wang Xinghao , Huang Mudan , Guo Yingyun 
Published: October 2023

ABSTRACT

Helicobacter pylori
(
H. pylori
) has been regarded as a definite carcinogenic bacterium for gastric cancer (GC). This multi-omics research was designed to investigate the genetic, microbial, and metabolic changes of GC patients when they are infected with
H. pylori
. We first mined The Cancer Genome Atlas Stomach Adenocarcinoma (STAD) data to identify the key genes and critical pathways in
H. pylori
-positive individuals with GC compared to
H. pylori
-negative individuals with GC. Then, fresh stool samples were collected from GC individuals screened for eligibility, and we analyzed the microbial changes and metabolite alterations between
H. pylori
-positive and
H. pylori
-negative GC individuals. Finally, we tried to explore the interaction between key gut flora and metabolite changes in GC patients infected with
H. pylori
. We identified three genes (GCG, APOA1, and IGFBP1) with significant relevance to
H. pylori
infection, and the survival monogram based on the three
H
.
pylori
-related genes showed good predictive ability for overall survival among GC individuals. 16S rRNA sequencing showed that the abundance of
Escherichia-Shigella, Bacteroides, Enterococcus
,
and Lactobacillus
was upregulated in GC cases with
H. pylori
at the level of genus. There exists a great difference in alpha and beta diversity between
H. pylori
group and non-
H
.
pylori
group. The untargeted metabolome analysis identified 295 significant fecal metabolites, and the levels of penitrem E, auberganol, stercobilinogen, and lys thr are upregulated in the
H. pylori
group. Finally, correlation analysis showed that there exists a significant correlation between the fecal metabolites and gut bacterial strains. This is the first clinical research to investigate the difference between GC patients with
H. pylori
and GC patients without
H. pylori
via multi-omics analysis. 16S rRNA sequencing along with untargeted metabolomics demonstrated decreased microbial diversity and metabolic dysregulation in gastric carcinoma individuals with
H. pylori
infection.

IMPORTANCE

This is the first clinical research to systematically expound the difference between gastric cancer (GC) individuals with
Helicobacter pylori
and GC individuals without
H. pylori
from the perspective of multi-omics. This clinical study identified significant genes, microbes, and fecal metabolites, which exhibited nice power for differentiating GC individuals with
H. pylori
infection from GC individuals without
H. pylori
infection. This study provides a crucial basis for a better understanding of eradication therapy among the GC population.
Brucella
MucR acts as an H-NS-like protein to silence virulence genes and structure the nucleoid
Authors: Barton Ian S. , Ren Zhongqing , Cribb Connor B. , Pitzer Joshua E. , Baglivo Ilaria , Martin Daniel W. , Wang Xindan , Roop R. Martin 
Published: October 2023

ABSTRACT

Histone-like nucleoid structuring (H-NS) and H-NS-like proteins serve as global gene silencers and work with antagonistic transcriptional activators (counter-silencers) to properly coordinate the expression of virulence genes in pathogenic bacteria. In
Brucella
, MucR has been proposed as a novel H-NS-like gene silencer, but direct experimental evidence is lacking. Here, we show that MucR serves as an H-NS-like silencer of the
Brucella abortus
genes encoding the polar autotransporter adhesins BtaE and BmaC, the c-di-GMP-specific phosphodiesterase BpdB, and the quorum-sensing regulator BabR. We also demonstrate that the MarR-type transcriptional activator MdrA can displace MucR from the
btaE
promoter, supporting the existence of MucR counter-silencers in
Brucella
. Moreover, our chromatin immunoprecipitation (ChIP)-seq analysis identified 546 MucR enrichment peaks along the genome, including in the promoters of the genes encoding the Type IV secretion machinery and effectors and the quorum-sensing regulator VjbR. Importantly, MucR ChIP-seq peaks overlap with the previously described binding sites for the transcriptional activators VjbR, BvrR, and CtrA suggesting that these regulators serve as MucR counter-silencers and work in concert with MucR to coordinate virulence gene expression in
Brucella
. In addition, using chromosome conformation capture (Hi-C), we show that like H-NS in
Escherichia coli
, MucR alters the global structure of the
Brucella
nucleoid. Finally, a copy of the
E. coli hns
rescues the distinctive growth defect and elevated
btaE
expression of a
B. abortus mucR
mutant. Together, these findings solidify the role of MucR as a novel type of H-NS-like protein and suggest that MucR’s gene-silencing properties play a key role in virulence in
Brucella
.

IMPORTANCE

Histone-like nucleoid structuring (H-NS) and H-NS-like proteins coordinate host-associated behaviors in many pathogenic bacteria, often through forming silencer/counter-silencer pairs with signal-responsive transcriptional activators to tightly control gene expression.
Brucella
and related bacteria do not encode H-NS or homologs of known H-NS-like proteins, and it is unclear if they have other proteins that perform analogous functions during pathogenesis. In this work, we provide compelling evidence for the role of MucR as a novel H-NS-like protein in
Brucella
. We show that MucR possesses many of the known functions attributed to H-NS and H-NS-like proteins, including the formation of silencer/counter-silencer pairs to control virulence gene expression and global structuring of the nucleoid. These results uncover a new role for MucR as a nucleoid structuring protein and support the importance of temporal control of gene expression in
Brucella
and related bacteria.
Draft genome sequence of methicillin-resistant
Staphylococcus aureus
strain D1418m22 isolated from human wound pus
Authors: Iram Daraksha , Sansi Manish Singh , Puniya Anil Kumar , Meena Sunita , Vij Shilpa 
Published: October 2023

ABSTRACT

This study reveals the complete genome sequence of methicillin-resistant
Staphylococcus aureus
(MRSA) strain d1418m22, sourced from a Karnal, Haryana, human skin wound. Classified as community-associated MRSA, it features a 2.78-MB genome harboring
Staphylococcus
-specific genetic elements, encompassing 2,625 protein-coding genes and 18 antimicrobial resistance genes.
Complete genome sequence of Koutango virus strain DakAnD5443 isolated from
Tatera kempi
in 1968
Authors: Haila Gabriel J. , Plante Jessica A. , Widen Steven G. , Beasley David W. C. 
Published: October 2023

ABSTRACT
Koutango virus (KOUV), a close relative of West Nile virus, is highly neuroinvasive in animal models and has been associated with human disease. The complete genome of the KOUV prototype strain DakAnD5443 is reported here and may facilitate development of infectious clones for further characterization of this novel flavivirus.
De novo
assembly of
Roseomonas mucosa
isolated from patients with atopic dermatitis
Authors: Barbian Kent , Bruno Daniel , Sykora Lydia , Ricklefs Stacy , Chaudhary Prem Prashant , Beare Paul A. , Myles Ian A. , Martens Craig M. 
Published: October 2023

ABSTRACT

Roseomonas mucosa
is associated with the normal skin microflora. Here, we present
de novo
sequence assemblies from
R. mucosa
isolates obtained from the skin lesions of three atopic dermatitis patients.
Complete genome sequence of
Lacticaseibacillus paracasei
strain VHProbi M56, isolated from yogurt
Authors: Guo Chaoqun , Cui Hongchang , Wang Qian , Duan Zhi 
Published: October 2023

ABSTRACT

Lacticaseibacillus paracasei
strain VHProbi M56 is a probiotic strain that was isolated from yogurt. Here, we report the whole-genome sequence of this bacterium. The whole genome contains a chromosome and a plasmid.
Complete genome sequence of
Proteiniborus
sp. MB09-C3, isolated from the feces of the black soldier fly larvae
Authors: Qiu Wanling , Zhu Junyu , Shih Chao-Jen , Wu Yen-Chi , Lai Shu-Jung , You Yi-Ting , Wu Chih-Hung , Liao Ching-Hua , Lin Haozhe , Li Shilong , Yan Yujia , Luo Xuming , Li Yin , Chen Sheng-Chung 
Published: October 2023

ABSTRACT

Here, we report the complete genome sequence of
Proteiniborus
sp. MB09-C3 (= BCRC 81405), isolated from the feces of black soldier fly (
Hermetia illucens
) larvae. The genome of strain MB09-C3 was selected for further species delineation and comparative genomic analysis.
Draft genome sequence of
Staphylococcus gallinarum
MTR_B001 strain isolated from breast muscle of a chicken in Bangladesh
Authors: Islam Md. Saiful , Ballah Fatimah Muhammad , Hoque M. Nazmul , Rahman A. M. M. Taufiquer , Rahman Md. Tanvir 
Published: October 2023

ABSTRACT

We announce the genome sequence of the
Staphylococcus gallinarum
MTR_B001 strain isolated from the breast muscle of a chicken in 2022 in Bangladesh. This assembled genome had an estimated length of 2,889,393 bp (with 50× genome coverage), 15 contigs, 36 predicted antibiotic resistance genes, and 27 predicted virulence factor genes.
The complete genome sequence of
Pantoea agglomerans
NBBC-01, isolated from rot potato tubers
Authors: Wang Yueying , Chen Ling , Liu Fang , Zhang Zhigang , Zhang Fei , Wang Kaimei , Fang Wei 
Published: October 2023

ABSTRACT

The Gram-negative bacterium
Pantoea agglomerans
has been isolated from various habitats including disease plants. Here, we present the genome of
P. agglomerans
strain NBBC-01 obtained from rotten potatoes that were infected by
Ditylenchus desstructor
. The genome information will prove advantageous in elucidating its ecological role.
Whole-genome sequence of a genotype VIII infectious bronchitis virus isolated from California layer chickens in 2021
Authors: Jude Rachel , da Silva Ana P. , Rejmanek Daniel , Crossley Beate , Jerry Carmen , Stoute Simone , Gallardo Rodrigo 
Published: October 2023

ABSTRACT
Herein, we report the complete genome for an avian infectious bronchitis virus isolated from cecal tonsils of California layers in 2021. This whole-genome sequence belongings to genotype GVIII, previously classified as a unique variant.