bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytosulfokine promotes fruit ripening and quality via phosphorylation of transcription factor DREB2F in tomato
Authors: Fang Hanmo , Zuo Jinhua , Ma Qiaomei , Zhang Xuanbo , Xu Yuanrui , Ding Shuting , Wang Jiao , Luo Qian , Li Yimei , Wu Changqi , Lv Jianrong , Yu Jingquan , Shi Kai 
Published: January 2024

Abstract
Phytosulfokine (PSK), a plant peptide hormone with a wide range of biological functions, is recognized by its receptor PHYTOSULFOKINE RECEPTOR 1 (PSKR1). Previous studies have reported that PSK plays important roles in plant growth, development, and stress responses. However, the involvement of PSK in fruit development and quality formation remains largely unknown. Here, using tomato (Solanum lycopersicum) as a research model, we show that exogenous application of PSK promotes the initiation of fruit ripening and quality formation, while these processes are delayed in pskr1 mutant fruits. Transcriptomic profiling revealed that molecular events and metabolic pathways associated with fruit ripening and quality formation are affected in pskr1 mutant lines and transcription factors are involved in PSKR1-mediated ripening. Yeast screening further identified that DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2F (DREB2F) interacts with PSKR1. Silencing of DREB2F delayed the initiation of fruit ripening and inhibited the promoting effect of PSK on fruit ripening. Moreover, the interaction between PSKR1 and DREB2F led to phosphorylation of DREB2F. PSK improved the efficiency of DREB2F phosphorylation by PSKR1 at the tyrosine-30 site, and the phosphorylation of this site increased the transcription level of potential target genes related to the ripening process and functioned in promoting fruit ripening and quality formation. These findings shed light on the involvement of PSK and its downstream signaling molecule DREB2F in controlling climacteric fruit ripening, offering insights into the regulatory mechanisms governing ripening processes in fleshy fruits.
Auxin receptor OsTIR1 mediates auxin signaling during seed filling in rice
Authors: Wu Daxia , Cao Yanan , Wang Daojian , Zong Guoxinan , Han Kunxu , Zhang Wei , Qi Yanhua , Xu Guohua , Zhang Yali 
Published: January 2024

Abstract
Cereal endosperm represents the most important source of the world’s food. Nevertheless, the molecular mechanisms behind sugar import into rice (Oryza sativa) endosperm and their relationship with auxin signaling are poorly understood. Here, we report that auxin transport inhibitor response 1 (TIR1) plays an essential role in rice grain yield and quality via modulating sugar transport into endosperm. The fluctuations of OsTIR1 transcripts parallel to the early stage of grain expansion among those of the 5 TIR1/AFB (auxin-signaling F-box) auxin co-receptor proteins. OsTIR1 is abundantly expressed in ovular vascular trace, nucellar projection, nucellar epidermis, aleurone layer cells, and endosperm, providing a potential path for sugar into the endosperm. Compared to wild-type (WT) plants, starch accumulation is repressed by mutation of OsTIR1 and improved by overexpression of the gene, ultimately leading to reduced grain yield and quality in tir1 mutants but improvement in overexpression lines. Of the rice AUXIN RESPONSE FACTOR (ARF) genes, only the OsARF25 transcript is repressed in tir1 mutants and enhanced by overexpression of OsTIR1; its highest transcript is recorded at 10 d after fertilization, consistent with OsTIR1 expression. Also, OsARF25 can bind the promoter of the sugar transporter OsSWEET11 (SWEET, sugars will eventually be exported transporter) in vivo and in vitro. arf25 and arf25/sweet11 mutants exhibit reduced starch content and seed size (relative to the WTs), similar to tir1 mutants. Our data reveal that OsTIR1 mediates sugar import into endosperm via the auxin signaling component OsARF25 interacting with sugar transporter OsSWEET11. The results of this study are of great significance to further clarify the regulatory mechanism of auxin signaling on grain development in rice.
Phthalic Acid and Its Role in Roots of Melon Plants (Cucumis melo)
Authors: Marquez Sixto A. , Crosby Kevin M. , Patil Bhimanagouda S. , Ibrahim Amir M. H. , Avila Carlos A. , Pessoa Herika P. , Singh Jashbir 
Published: January 2024

Melon is an important crop worldwide. However, this crop has many problems, such as high production costs, pests, diseases, and sensitivity to abiotic stresses. Among the diseases that negatively impact its production, vine decline disease (VDD), caused by the fungus Monosporascus cannonballus, is very dangerous because it affects the plant when the fruit is almost ready to be harvested, which causes huge losses to growers. In addition, this disease is widely spread in areas where melons are grown all over the world, and its control through soil fumigation is costly and polluting. Thus, cheap and sustainable ways to combat it must be found. Therefore, this study was conducted with the objectives of identifying and quantifying phenolic compounds produced during the interaction of melon plants with the fungus M. cannonballus, which can be useful in controlling VDD, ameliorating cultural practices of this crop, and consequently reducing production costs. Two varieties were grown and inoculated with the fungus M. cannonballus: TAM-Uvalde (susceptible) and USDA PI 124104 (resistant). Their roots were sampled before inoculating with the fungus (0 h) and 24, 48, and 72 h after inoculation. The root chemicals were then analyzed using high-performance liquid chromatography. Our results indicate that phthalic acid was induced in the roots after inoculation in both varieties, which suggests that it is produced by the plant as a defensive compound. Also, the production of phthalic acid varied over time according to the variety used, which suggests its allelopathic function. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Parenting Expectations, NICU Experiences, and Maternal Psychological Outcomes
Authors: Rodrigues Sarah M. , Shin Sanghyuk S. , Pinto Melissa D. , Bounds Dawn T. , Terry Jennifer , Burton Candace W. 
Published: January 2024

Background:
Infant neonatal intensive care unit (NICU) hospitalization increases maternal risk for psychological distress. However, no universal screening standards exist and predicting maternal risk remains challenging. Reconceptualizing maternal distress in relation to differences between parenting expectations and NICU experiences may illuminate commonalities across a range of experiences.

Purpose:
This study explored parenting expectation–experience differences (EEDs) among NICU mothers and assessed correlations between EED scores and psychological outcomes 1 to 5 years post-NICU hospitalization.

Methods:
A 3-phase explanatory sequential mixed-methods design was used. Pearson's correlation coefficients were used to measure relationships between EED scores and maternal psychological outcomes. Reflexive thematic analysis of one-on-one, semi-structured interviews contextualized EED scores.

Results:
Most participants (92.9%) reported negative EED scores, indicating NICU experiences fell short of parenting expectations. Significant inverse correlations were found between EED scores and maternal outcomes, including depression (r = −0.25, P < .01), anxiety (r = −0.25, P < .01) and posttraumatic stress disorder symptoms (r = −0.41, P < .001), and perceived parenting self-efficacy (r = −0.28, P < .01). Major qualitative themes included unexpected versus prepared, lost parenting experiences, and surviving and thriving. Data synthesis contextualized EED scores and revealed key differences in meaning ascribed to unmet parenting expectations.

Implications for Practice and Research:
Preparing mothers for infant NICU hospitalization and creating a NICU parenting environment, which better supports mothers and their engagement in parenting tasks, may help to reduce differences between parenting expectations and NICU experiences. Further research is needed to elucidate the impacts of parenting EEDs in this population.
The Effect of Social Isolation on 1-Year Outcomes After Surgical Repair of Low Energy Hip Fracture
Authors: Mandl Lisa A. , Rajan Mangala , Lipschultz Robyn A. , Lian Serena , Sheira Dina , Frey Marianna B. , Shea Yvonne M. , Lane Joseph M. 
Published: January 2024

OBJECTIVES:
To evaluate if social isolation or loneliness is associated with outcomes 1 year after low energy hip fracture.

METHODS:

Design:
Prospective inception cohort study.

Setting:
Academic Level I Trauma Center.

Patient Selection Criteria:
Participants were ≥ 65 years of age and enrolled 2-4 days after surgery for a first low energy hip fracture. Exclusion criteria were bilateral or periprosthetic hip fracture, previous hip fracture, non-English speaking, international address, active cancer, stage 4 cancer in the past 5 years, radiation to the hip region and cognitive impairment. Participants were followed longitudinally for one year.

Outcome Measures and Comparisons:
The PROMIS-29 was elicited 2-4 days post-operatively and 1 year later. Patient reported risk factors included the Lubben Social Networks Scale and the UCLA Loneliness Scale, which were compared to the Lower Extremity Activity Scale and PROMIS-29 domains.

RESULTS:
Three hundred and twenty-five patients were enrolled. Participants had a median age of 81.7 years, were 70.9 % female and were 85.9% white. 31.6% of patients were socially isolated at time of fracture. At 1 year, 222 of the 291 subjects who were confirmed alive at one year provided data. Multivariable linear models were performed separately for each outcome, including Lower Extremity Activity Scale and PROMIS-29 domains. Controlling for age, sex, education, and body mass index, those who were socially isolated at time of fracture had worse PROMIS-29 function (β= -3.83 p= 0.02) and ability to participate in social roles (β= -4.17 p= 0.01) at 1 year. Secondary analyses found that pre-fracture loneliness was associated with clinically meaningfully worse function, anxiety, depression, fatigue, sleep, pain and ability to participate in social roles at 1 year, (all p< 0.01).

CONCLUSIONS:
Pre-fracture social isolation was associated with worse outcomes 1 year after surgical repair of low energy hip fracture. This data suggests loneliness may be more strongly associated with important patient centric metrics than pre-fracture social isolation. Given the dearth of modifiable risk factors in this population, future studies are needed to evaluate whether improving social connections could impact outcomes in this rapidly growing demographic.
E2F3/5/8 serve as potential prognostic biomarkers and new therapeutic direction for human bladder cancer
Authors: Gan Zhilu , Abudurexiti Alimujiang , Hu Xiaogang , Chen Wenxin , Zhang Ning , Sang Wei 
Published: January 2024

Objects:
Human bladder cancer (BC) is the most common urogenital system malignancy. E2F transcription factors (E2Fs) have been reported to be involved in the growth of various cancers. However, the expression patterns, prognostic value and immune infiltration in the tumor microenvironment of the 8 E2Fs in BC have yet fully to be explored.

Methods and strategy:
We investigated the differential expression of E2Fs in BC patients, the prognostic value and correlation with immune infiltration by analyzing a range of databases.

Results:
We found that the mRNA expression levels of E2F1/2/3/4/5/7/8 were significantly higher in BC patients than that of control tissues. And the increased mRNA expression levels of all E2Fs were associated with tumor stage of BC. The survival analysis revealed that the elevated mRNA expression levels of E2F3/5/8 were significantly correlated with the overall survival (OS) of BC patients. And the genetic changes of E2Fs in BC patients were associated with shorter overall survival (OS) and progression-free survival (PFS). In addition, we revealed that the E2F3/5/8 expressions were closely correlated with tumor-infiltrating lymphocytes (TILs).

Conclusions:
E2F3/5/8 might serve as promising prognostic biomarkers and new therapeutic direction for BC patients.
Knowledge domain and emerging trends of autophagy in cardiovascular research: A bibliometric analysis
Authors: Lv Meng , Zhu Xueping , You Yaping , Fan Shaowei , Chai Ruoning , Cheng Tao , Xue Wenjing , Shi Shuai , Hu Yuanhui 
Published: January 2024

Background:
Autophagy is essential for the homeostasis and function of the cardiovascular system. Citespace is a visual analysis software developed in the context of scientometrics and data visualization. The purpose of this study is to use Citespace software to conduct bibliometric and visual analysis of the research on autophagy in cardiovascular diseases, identify the current status, hot spots and trends in this field, help researchers clarify the future research focus and direction of autophagy in cardiovascular diseases, and provide more positive and broader ideas for the treatment and drug development of cardiovascular diseases.

Methods:
In the Web of Science Core Collection database to download the data from 2004 to 2022 regarding autophagy in cardiovascular research. CitespaceV was used to collect the research status, hotspots and development trends for visual analysis.

Results:
The 3568 articles were published by 547 authors from 397 institutions in 75 countries. From 2004 to 2021, the annual publications increased over time. The top 3 productive nations were China, the United States, and Germany. The leading institution was China’s Fudan University. The most cited paper is Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). The research hotpots include monitoring methods for autophagy activity, changes in autophagy levels in different types of cardiovascular diseases, autophagy signal transduction mechanism in cardiovascular diseases, etc.

Conclusion:
Bibliometric analysis provided valuable information for autophagy research in cardiovascular disease, which is full of opportunities and challenges. The research of autophagy in the field of cardiovascular diseases is still worthy of in-depth exploration. A challenge with autophagy-targeted therapies is their dichotomy in which the goal is to target maladaptive autophagy while maintaining a baseline level of cell survival to optimize a beneficial outcome. It is necessary for scientists to develop new methods to evaluate the level of autophagy from basic application to human body and reveal the signaling mechanism of autophagy in different types of cardiovascular diseases.
Study on the application of quality control circle activity in postoperative nursing of elderly dental implants
Authors: Zhao Hongxuan , Hu Meichun , Cao Yannan , Wu Xiangbing , Zhu Fangyong 
Published: January 2024

Background:
To explore the application effect of “quality control circle” activity in postoperative nursing of elderly dental implants.

Methods:
From January 2022 to December 2022, a total of 60 elderly patients were selected and divided into an experimental group and a control group, with 30 cases in each group using the random number table method. The elderly patients with dental implants received postoperative care under the supervision of a quality control circle. The control group was treated with conventional nursing methods. The success rate, postoperative complications, and satisfaction of the 2 groups were compared.

Results:
By comparing the postoperative care effect of the 2 groups of patients, the satisfaction of the experimental group and the control group was 93.3% and 73.3%, respectively, showing the satisfaction of the experimental group was higher than the control group (P < .05). The planting success rate of the experimental and control groups were 96.7% and 66.7%, respectively, with the experimental group outperforming the control group (P < .05). The proportion of complications in the experimental was found to be fewer (6.7%) than in the control group (33.3%) (P < .05).

Conclusion:
Quality control circle activities can effectively improve the success rate of dental implants, reduce the occurrence of postoperative complications, improve patient satisfaction, and help medical staff in providing better treatment for patients.
Treatment outcomes amongst older people with HIV infection receiving antiretroviral therapy
Authors: Li Na , Zheng Hong-Yi , He Wen-Qiang , He Xiao-Yan , Li Rui , Cui Wen-Bo , Yang Wei-Lin , Dong Xing-Qi , Shen Zhi-Qiang , Zheng Yong-Tang 
Published: January 2024

Objectives:
There is conflicting data regarding the response of older people with HIV (PWH) to antiretroviral therapy (ART). The objective of this study was to evaluate the long-term immunological and virological responses, changes in regimen, and adverse drug reactions (ADRs) in older participants (50+ years) compared with younger (18–34 years) and middle-aged (35–49 years) PWH.

Methods:
A retrospective review of medical records was conducted on 1622 participants who received ART in Yunnan Province, China, from 2010 to 2019. The study compared CD4+ T-cell counts, CD4+/CD8+ ratio, and relative numbers between different groups using the Kruskal–Wallis test. Cox proportional hazards regression models were used to identify variables associated with the occurrence of immune reconstitution insufficiency. The rates of immune reconstitution, incidence of ADRs, and rates of treatment change were analyzed using the chi-squared test or Fisher's exact test.

Results:
Over 95% achieved viral load 200 copies/ml or less, with no age-related difference. However, older participants exhibited significantly lower CD4+ T-cell counts and CD4+/CD8+ recovery post-ART (P < 0.001), with only 32.21% achieving immune reconstitution (compared with young: 52.16%, middle-aged: 39.29%, P < 0.001) at the end of follow-up. Middle-aged and elderly participants changed ART regimens more because of ADRs, especially bone marrow suppression and renal dysfunction.

Conclusion:
Although the virological response was consistent across age groups, older individuals showed poorer immune responses and higher susceptibility to side effects. This underscores the need for tailored interventions and comprehensive management for older patients with HIV.
HIV viral suppression in children and adolescents 2 years after transition to dolutegravir: a multicentre cohort study
Authors: Devendra Akash , Kohler Maurus , Letsika Motlatsi , Khooa Hape , Motaboli Lipontso , Lerotholi Malebanye , Tschumi Nadine , Labhardt Niklaus D. , Brown Jennifer A. 
Published: January 2024

Background:
Treatment failure is common among children and adolescents with HIV. Antiretroviral therapy (ART) containing dolutegravir has recently been rolled out across Africa, though long-term real-world data in paediatric populations are lacking. Here, we report treatment outcomes among children and adolescents in Lesotho who transitioned from nonnucleoside reverse transcriptase inhibitor (NNRTI)-based to dolutegravir-based ART through 2 years’ follow-up.

Methods:
Data were derived from two open cohort studies in Lesotho. Children and adolescents aged less than 18 years who transitioned from NNRTI-based to dolutegravir-based ART at least 18 months before data closure were included. We report viral load results less than 12 months before, 12 (window: 6–17) months after, and 24 (window: 18–29) months after transition to dolutegravir. Associations of pretransition demographic and clinical factors with 24-month viraemia were assessed through multivariable logistic regression.

Results:
Among 2126 included individuals, 1100 (51.7%) were female individuals, median age at transition to dolutegravir was 14.0 years [interquartile range (IQR) 11.5–15.8], and median time taking ART at transition was 7.6 years (IQR 4.4–10.6). Among those with a viral load result at the respective time points, viral suppression to less than 50 copies/ml was achieved by 1635 of 1973 (82.9%) less than 12 months before, 1846 of 2012 (91.8%) 12 months after, and 1725 of 1904 (90.6%) 24 months after transition to dolutegravir. Pretransition viraemia was associated with viraemia at 24 months, though more than 80% of individuals with pretransition viraemia achieved resuppression to less than 50 copies/ml at 24 months.

Conclusion:
The proportion of children and adolescents with viral suppression increased after transition to dolutegravir, though further progress is needed to reach global targets.
Prevalence and Correlates of Intimate Partner Violence Victimization Among Urban Adolescents and Young Adults Living With Perinatally-Acquired HIV Infection or Perinatal HIV Exposure
Authors: Attoh-Okine Naa-Djama , Corbeil Tom , Poku Ohemaa , Kluisza Luke , Liotta Lucy , Morrison Corey , Dolezal Curtis , Robbins Reuben N. , Kreniske Philip , Abrams Elaine J. , Wiznia Andrew , Mellins Claude A. 
Published: January 2024

Introduction:
Because of privacy and ethical concerns, the data cannot be made available because of the sensitivity of the HIV data and the relatively small sample and ease of identifying people if a few demographics are known.
Few studies have examined intimate partner violence (IPV) victimization among adolescents and young adults (AYAs) with perinatally acquired HIV-infection (PHIV) or perinatal HIV exposure without infection (PHEU) in the United States. The purpose of this study was to (1) estimate lifetime and past-year prevalence of IPV victimization and (2) examine correlates of IPV victimization by subtype (physical, psychological, and sexual) and severity (low, moderate, and severe).

Methods:
Data came from the sixth interview of an ongoing New York City–based longitudinal study of primarily Black and Latinx AYAPHIV and AYAPHEU. We examined 232 participants (142 PHIV; 90 PHEU) who had reported having been in at least 1 romantic relationship. We used logistic regression models to explore the association between IPV victimization outcomes and select sociodemographic, psychiatric, and environmental factors. Models were adjusted for age, gender, race, ethnicity, and HIV status.

Results:
IPV victimization prevalence was 84% for lifetime and 65% for the past year. There were no differences in IPV victimization prevalence by PHIV status. Having a recent substance use disorder, reporting higher levels of neighborhood stress, and being male were all positively associated with at least 1 IPV outcome; stronger familial relationships exhibited a protective effect.

Conclusions:
The present study suggests that the prevalence of IPV victimization among AYAPHIV and AYAPHEU is exceedingly high that warrants targeted IPV screening and programming for this population.