bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteoporosis and osteoarthritis: a bi-directional Mendelian randomization study
Authors: Qu Yudun , Chen Shibo , Han Mengling , Gu Ziqi , Zhang Yujie , Fan Tianxiang , Zeng Muhui , Ruan Guangfeng , Cao Peihua , Yang Qian , Ding Changhai , Zhang Yan , Zhu Zhaohua 
Published: December 2023

Abstract
Objective
To investigate the causal relationship between low bone mineral density (BMD) and osteoarthritis (OA) using Mendelian randomization (MR) design.

Methods
Two-sample bi-directional MR analyses were performed using summary-level information on OA traits from UK Biobank and arcOGEN. Sensitivity analyses including MR-Egger, simple median, weighted median, MR pleiotropy residual sum, and outlier approaches were utilized in conjunction with inverse variance weighting (IVW). Gene ontology (GO) enrichment analyses and expression quantitative trait locus (eQTL) colocalization analyses were used to investigate the potential mechanism and shared genes between osteoporosis (OP) and OA.

Results
The IVW method revealed that genetically predicted low femoral neck BMD was significantly linked with hip (β = 0.105, 95% CI: 0.023–0.188) and knee OA (β = 0.117, 95% CI: 0.049–0.184), but not with other site-specific OA. Genetically predicted low lumber spine BMD was significantly associated with OA at any sites (β = 0.048, 95% CI: 0.011–0.085), knee OA (β = 0.101, 95% CI: 0.045–0.156), and hip OA (β = 0.150, 95% CI: 0.077–0.224). Only hip OA was significantly linked with genetically predicted reduced total bone BMD (β = 0.092, 95% CI: 0.010–0.174). In the reverse MR analyses, no evidence for a causal effect of OA on BMD was found. GO enrichment analysis and eQTL analysis illustrated that DDN and SMAD-3 were the most prominent co-located genes.

Conclusions
These findings suggested that OP may be causally linked to an increased risk of OA, indicating that measures to raise BMD may be effective in preventing OA. More research is required to determine the underlying processes via which OP causes OA.
New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologies
Authors: Zekušić Marija , Bujić Mihica Marina , Skoko Marija , Vukušić Kruno , Risteski Patrik , Martinčić Jelena , Tolić Iva M. , Bendelja Krešo , Ramić Snježana , Dolenec Tamara , Vrgoč Zimić Ivana , Puljić Dominik , Petric Vicković Ivanka , Iveković Renata , Batarilo Ivanka , Prosenc Zmrzljak Uršula , Hoffmeister Alan , Vučemilo Tiha 
Published: December 2023

Abstract
Background
Limbal stem cells (LSCs) are crucial for the regeneration of the corneal epithelium in patients with limbal stem cell deficiency (LSCD). Thus, LSCs during cultivation in vitro should be in highly homogeneous amounts, while potency and expression of stemness without tumorigenesis would be desirable. Therefore, further characterization and safety evaluation of engineered limbal grafts is required to provide safe and high-quality therapeutic applications.

Methods
After in vitro expansion, LSCs undergo laboratory characterization in a single-cell suspension, cell culture, and in limbal grafts before transplantation. Using a clinically applicable protocol, the data collected on LSCs at passage 1 were summarized, including: identity (cell size, morphology); potency (yield, viability, population doubling time, colony-forming efficiency); expression of putative stem cell markers through flow cytometry, immunofluorescence, and immunohistochemistry. Then, mitotic chromosome stability and normal mitotic outcomes were explored by using live-cell imaging. Finally, impurities, bacterial endotoxins and sterility were determined.

Results
Expression of the stemness marker p63 in single-cell suspension and in cell culture showed high values by different methods. Limbal grafts showed p63-positive cells (78.7 ± 9.4%), Ki67 proliferation (41.7 ± 15.9%), while CK3 was negative. Impurity with 3T3 feeder cells and endotoxins was minimized. We presented mitotic spindles with a length of 11.40 ± 0.54 m and a spindle width of 8.05 ± 0.55 m as new characterization in LSC culture. Additionally, live-cell imaging of LSCs (n = 873) was performed, and only a small fraction < 2.5% of aberrant interphase cells was observed; 2.12 ± 2.10% of mitotic spindles exhibited a multipolar phenotype during metaphase, and 3.84 ± 3.77% of anaphase cells had a DNA signal present within the spindle midzone, indicating a chromosome bridge or lagging chromosome phenotype.

Conclusion
This manuscript provides, for the first time, detailed characterization of the parameters of fidelity of the mitotic process and mitotic spindle morphologies of LSCs used in a direct clinical application. Our data show that p63-positive CK3-negative LSCs grown in vitro for clinical purposes undergo mitotic processes with extremely high fidelity, suggesting high karyotype stability. This finding confirms LSCs as a high-quality and safe therapy for eye regeneration in humans.
The clinical association between coagulation indexes, platelet-related parameters, and bone metastasis of newly diagnosed prostate cancer
Authors: Yu Zhiwei , Yuan Mingxue , Chen Guojun 
Published: December 2023

Abstract
Background
At present, much evidence shows that many cancers have a high risk of thrombosis. Several studies have shown the prognostic value of platelet-related parameters and coagulation indexes in prostate cancer (PCa). However, the association between platelet-related parameters, coagulation indexes and bone metastasis of Pca is unclear.

Methods
A total of 234 pathologically diagnosed patients with Pca were consecutively collected and stratified into the bone metastasis group and non-bone metastasis group according to the results of the bone scan. ROC curve analysis was used to explore the auxiliary predictive value of single and combined parameters for bone metastasis in Pca patients. Univariate and multivariate Logistic regression analyses were used to determine the relationship between platelet-related parameters, coagulation indexes, and bone metastasis of Pca.

Results
Platelet count (PLT), fibrinogen (Fib), prostate-specific antigen (PSA), and D-dimer (DD) levels of the bone metastasis group were significantly higher than the non-bone metastasis group (P = 0.010, P < 0.001, P < 0.001, and P < 0.001, respectively). This study confirmed that PLT, PSA, DD and Fib have auxiliary predictive value for prostate cancer bone metastasis. After the combination of PLT, PSA, DD and Fib, the area under the curve, sensitivity and specificity increased significantly. The univariate logistic analysis demonstrated that PLT (OR: 1.008, P = 0.011), DD (OR: 2.690, P < 0.001), PSA (OR: 1.073, P < 0.001), Gleason score (OR: 7.060, P < 0.001), and Fib (OR: 2.082, P < 0.001) were significantly positively correlated with bone metastasis of Pca. Multivariate analysis showed that PSA (OR: 1.075, P < 0.001), DD (OR: 2.152, P < 0.001), Gleason score (OR: 2.904, P < 0.001), and Fib (OR: 1.706, P < 0.001) were independent risk factors for bone metastasis of Pca after adjusting for Age, BMI and other confounding factors.

Conclusions
Higher platelet, D-dimer, prostate-specific antigen, Gleason score, and fibrinogen levels may predict a worse prognosis in patients with Pca. PLT, DD, and Fib, as readily available and relatively inexpensive indicators, help predict bone metastasis of Pca. It is suggested that PLT, DD and Fib may be helpful in the risk stratification of Pca.
Case report: first data on the foraging behaviour of velvet scoters (Melanitta fusca) in soft-bottom coastal waters of the southeast Baltic Sea
Authors: Forni Paola , Morkunas Julius , Daunys Darius 
Published: December 2023

Abstract
Background
The foraging behaviour of sea ducks has always been difficult to study due to their exclusive occupancy of coastal waters during the wintering season. In this study we investigated the foraging behaviour of seven wintering velvet scoters (Melanitta fusca) in Lithuanian soft-bottom coastal waters by attaching a solar-powered external GPS‐GSM transmitter to each individual bird. This transmitter data allowed us to analyse the dive phase parameters and feeding depths during December 2021 and March 2022.

Results
The results showed that velvet scoters dived deeper than a 30-m depth, with the dive duration lasting up to 90 s on average. The bottom duration increased rapidly in the first 5 m and then levelled off at around 40 s for deeper waters. The underwater duration varied between 2 and 7.5 h a day. The dive effort (as total underwater time) and the bottom duration of velvet scoters were related to the depth and prey biomass, although both factors were demonstrated to be interdependent. Moreover, the results showed that velvet scoters were less active in December than in March in terms of both average diving duration and diving frequency. The results also confirmed the high diurnal activity of the velvet scoter, although the birds demonstrated night activity in December and not in March.

Conclusion
This information on the velvet scoter’s foraging behaviour may have important implications for the conservation of the species, and provides new insights into the improvement of conservation measures for the species in the Baltic Sea.
Phagocytic cell death leads to enhanced release of pro-inflammatory S100A12 in familial Mediterranean fever
Authors: Varga G. , Schleifenbaum S. , Koenig U. , Waldkirch J. , Hinze C. , Kessel C. , Geluk W. , Pap T. , Lainka Elke , Kallinich Tilmann , Foell D. , Wittkowski H. 
Published: December 2023

Abstract
Background
Familial Mediterranean fever (FMF) is a prototypical autoinflammatory syndrome associated with phagocytic cell activation. Pyrin mutations are the genetic basis of this disease, and its expression has been shown in monocytes, granulocytes, dendritic cells, and synovial fibroblasts. Pyrin functions as a cytosolic pattern recognition receptor and forms a distinct pyrin inflammasome. The phagocyte-specific protein S100A12 is predominantly expressed in granulocytes and belongs to the group of damage associated molecular patterns (DAMP). S100A12 can be detected at massively elevated levels in the serum of FMF patients, even in clinically inactive disease. Whether this is crucial for FMF pathogenesis is as yet unknown, and we therefore investigated the mechanisms of S100A12 release from granulocytes of FMF patients presenting clinically inactive.

Results
We demonstrate that FMF neutrophils from patients in clinical inactive disease possess an intrinsic activity leading to cell death even in exogenously unstimulated neutrophils. Cell death resembles NETosis and is dependent on ROS and pore forming protein gasdermin D (GSDMD), as inhibitors for both are capable of completely block cell death and S100A12 release. When pyrin-activator TcdA (Clostridium difficile toxin A) is used to stimulate, neutrophilic cell death and S100A12 release are significantly enhanced in neutrophils from FMF patients compared to neutrophils from HC.

Conclusions
We are able to demonstrate that activation threshold of neutrophils from inactive FMF patients is decreased, most likely by pre-activated pyrin. FMF neutrophils present with intrinsically higher ROS production, when cultured ex vivo. This higher baseline ROS activity leads to increased GSDMD cleavage and subsequent release of, e.g., S100A12, and to increased cell death with features of NETosis and pyroptosis. We show for the first time that cell death pathways in neutrophils of inactive FMF patients are easily triggered and lead to ROS- and GSDMD-dependent activation mechanisms and possibly pathology. This could be therapeutically addressed by blocking ROS or GSDMD cleavage to decrease inflammatory outbreaks when becoming highly active.
Quantifying the influence of clay-bound water on wave dispersion and attenuation signatures of shale: An experimental study
Authors: Long Teng , Qin Xuan , Wei Qianqian , Zhao Luanxiao , Wang Yang , Chen Feng , Myers Michael T. , Zheng Yingcai , Han De-Hua 
Published: December 2023

Understanding the elastic and attenuation signatures of shales is of considerable interest for unconventional reservoir characterization and sealing capacity evaluation for CO2 sequestration and nuclear waste disposal. We have conducted laboratory measurements on seven shale samples at seismic frequencies (2–100 Hz) to study the effects of clay-bound water (CBW) on their wave dispersion and attenuation signatures. With nuclear magnetic resonance and a helium porosimeter, the volume of CBW in the shale samples is quantified. The forced-oscillation measurement reveals that Young’s modulus exhibits a continuous dispersion trend from 2 to 100 Hz. The extensional attenuation ([Formula: see text]) shows a weak frequency and pressure dependence on effective pressure ranging from 5 to 35 MPa. The magnitude of extensional attenuation shows a positive correlation with CBW, with an [Formula: see text] value of 0.89. It is found that 4% of CBW in the rock frame causes approximately a 5% modulus increase from 2 to 100 Hz. We adopt a constant [Formula: see text] model for assigning frequency-dependent bulk and shear moduli to the CBW in the rock-physics modeling, which can fit the experimental data of modulus dispersion and attenuation well, indicating that the bulk and shear moduli of CBW in shales might behave viscoelastically.
Complex association between post-COVID-19 condition and anxiety and depression symptoms
Authors: Tebeka Sarah , Carcaillon-Bentata Laure , Decio Valentina , Alleaume Caroline , Beltzer Nathalie , Gallay Anne , Lemogne Cédric , Pignon Baptiste , Makovski Tatjana T. , Coste Joël 
Published: December 2023

Abstract

Background
To assess the associations between anxiety and depressive symptoms and post-COVID-19 condition (PCC) by exploring the direction of these associations and their relevance in the definition of PCC.

Methods
Nationwide survey among French adults, recruited between March and April, 2022, using a quota method to capture a representative sample of the general population with regard to sex, age, socioeconomic status, size of the place of residence, and region. We included all participants who met the World Health Organization (WHO) definition of PCC in addition to a random sample of participants infected with SARS-COV-2 for at least 3 months but without PCC. Self-reported anxiety and depressive symptoms, chronic anxiety and depression (for more than 3 years), and anxiety and depression were measured using the GAD-2 and PHQ-2 questionnaires, respectively.

Results
In a sample of 1,095 participants with PCC and 1,021 participants infected with SARS-COV-2 without PCC, 21% had self-reported anxiety and 18% self-reported depression, whereas 33% and 20% had current measured symptoms of anxiety and depression, respectively. The high prevalence of these symptoms cannot only be explained by the characterization of PCC, as only 13.4% of anxiety symptoms and 7.6% of depressive symptoms met the WHO criteria for PCC. Only one participant met the WHO criteria based on self-reported anxiety or depressive symptoms alone, as these were always combined with other symptoms in patients with PCC. Chronic symptoms were associated with PCC (aOR 1.27; 95% CI: 1.00–1.61). In addition, measured anxiety was associated with PCC (aOR = 1.29; 95% CI: 1.02–1.62).

Conclusions
Pre-COVID-19 chronic anxiety and depression may play a role in the development of PCC or share vulnerability factors with it. Our results challenge the inclusion of anxiety and depression in the definition of PCC.
The effectiveness of mother-led infant massage on symptoms of maternal postnatal depression: A systematic review
Authors: Geary Orla , Grealish Annmarie , Bright Ann-Marie 
Published: December 2023

Introduction
Postnatal depression is a significant public health issue which may escalate and lead to adverse outcomes for women, infants, their family and the wider society. The aim of this review was to examine the effectiveness and experiences of mother-led infant massage on symptoms of maternal postnatal depression and to synthesise these findings to inform policy, practice and further research.

Methods
A systematic search of five academic databases was conducted: CINAHL, MEDLINE, EMBASE, PsycINFO and Allied and Complementary Medicine Database in February 2023 with no date or geographic limiters set owing to the paucity of research on this subject area. Quality appraisal was undertaken using the Joanna Briggs Institute quality appraisal tools and all included RCT’s were assessed separately using the Cochrane Risk of Bias Tool. Narrative synthesis was used to analyse the data.

Findings
A total of (n = 323) studies were returned of which (n = 8) met the inclusion criteria for the review. This review identified a total sampling of (n = 521) women with maternal postnatal depression. The results are presented under three themes: 1) the effectiveness of mother-led infant massage on symptoms of postnatal depression; 2) women’s experiences of mother-led infant massage; and 3) the effects of mother-led infant massage on the mother-infant relationship.

Discussion
The review highlights women who used infant massage displayed a reduction in symptoms of postnatal depression, improved mother-infant interactions and improved self-efficacy in addition to benefits for infants. Public Health Nurse/Community Midwife-led infant massage may help to relieve such symptoms and empower women.
Epidemiology, Comorbidities, and Treatment of Cyclic Vomiting Syndrome in the United States
Authors: Chen Yaozhu Juliette , Princic Nicole , Winer Isabelle , Richmond Camilla , Williams James , Thavamani Aravind , Levinthal David J. , Venkatesan Thangam 
Published: December 2023

INTRODUCTION:
Cyclic vomiting syndrome (CVS) imposes a substantial burden, but epidemiological data are scarce. This study aimed to estimate the incidence and prevalence of CVS, comorbid conditions, and treatment patterns, using administrative databases in the United States.

METHODS:
This cross-sectional study used claims data from Merative MarketScan Commercial/Medicare Supplemental and Medicaid databases in all health care settings. Incidence and prevalence rates for 2019 were calculated and stratified by age, sex, region, and race/ethnicity. Patient characteristics were reported among newly diagnosed patients with CVS (i.e., no documented claims for CVS before 2019). CVS was defined as having 1+ inpatient and/or 2+ outpatient CVS claims that were 7+ days apart.

RESULTS:
The estimated prevalence of CVS was 16.7 (Commercial/Medicare) and 42.9 (Medicaid) per 100,000 individuals. The incidence of CVS was estimated to be 10.6 (Commercial/Medicare) and 26.6 (Medicaid) per 100,000 individuals. Both prevalence and incidence rates were higher among female individuals (for both Commercial/Medicare and Medicaid). Comorbid conditions were common and included abdominal pain (56%–64%), anxiety (32%–39%), depression (26%–34%), cardiac conditions (39%–42%), and gastroesophageal reflux disease (30%–40%). Despite a diagnosis of CVS, only 32%–35% had prescriptions for prophylactic treatment and 47%–55% for acute treatment within the first 30-day period following diagnosis.

DISCUSSION:
This study provides the first population-level estimates of CVS incidence and prevalence in the United States. Comorbid conditions are common, and most patients with CVS do not receive adequate treatment. These findings underscore the need for improving disease awareness and developing better screening strategies and effective treatments.
The evidential meaning of presupposition and implicature between retractability and deniability of information
Authors: Masia Viviana 
Published: December 2023

Abstract
The relation evidentiality bears on the coding of some information as presupposition or as implicature is still an underexplored research field. In this paper, such an interplay is addressed by looking into how presupposed and implied contents (differently) respond to contexts of challenge and deniability. As taken for granted information (Stalnaker, Robert. 1973. Presuppositions. Journal of Philosophical Logic 2(4). 447–457), presupposition is more resistant to both challenging and retracting conversational moves, since it conveys content the speaker does not commit to. By contrast, implicature – characterized as intentional meaning (Grice, Herbert P. 1975. Logic and conversation. In Peter Cole & Jerry Morgan (eds.), Syntax and semantics 3: Speech acts, 41–58. New York: Academic Press) – allows both challenging and retracting conversational moves, because it is information the speaker commits to the most, similarly to what happens with plain declarative sentences. Building on this account, it is suggested that the higher challenging and deniability status cued by implicature is related to its function of encoding a direct type of evidentiality (Aikhenvald, Alexandra Y. 2004. Evidentiality. Oxford: Oxford University Press), namely a condition in which the speaker presents herself as the first-hand source of some information. Conversely, the weaker challengeability and deniability associated to presupposition hinges on its property of encoding a mutual type of evidentiality (Hintz, Daniel J. & Hintz Diane M. 2017. The evidential category of mutual knowledge in Quechua. Lingua 186. 88–109), that is, a state in which information is construed and conveyed as already shared by all participants at the moment of utterance.
Wood modification with trimethoxyphenylsilane via supercritical carbon dioxide for enhanced weathering resistance
Authors: Triquet Juliette , Renard Olivier , Piluso Pierre 
Published: December 2023

Abstract
This research focuses on increasing wood resistance against UV degradation using trimethoxyphenylsilane as modifying agent and supercritical CO2 as a green solvent and reaction medium. Trimethoxyphenylsilane was successfully grafted on European spruce (Picea abies, H. Karst) and was characterized by SEM-EDX and UV–vis reflectance. SEM-EDX showed the presence of silicium inside the wood cell wall. Increased wood moisture content showed positive effect on weight gain of trimethoxyphenylsilane after treatment. UV resistance of treated wood was evaluated by following the color change during artificial weathering. Modified samples showed improved weathering resistance compared to unmodified wood with a ΔE value around 5 after 260 h. Processing and modifying wood using supercritical CO2 is a promising route to increase wood performances and increase its use as a building material.
Intrafamilial phenotypic heterogeneity in siblings with pseudohypoparathyroidism 1B due to maternal STX16 deletion
Authors: Odom John , Bacino Carlos A. , Karaviti Lefkothea P. , Bi Weimin , Hoyos-Martinez Alfonso 
Published: December 2023

Abstract

Objectives
Pseudohypoparathyroidism (PHP1B) is most commonly caused by epigenetic defects resulting in loss of methylation at the GNAS locus, although deletions of STX16 leading to GNAS methylation abnormalities have been previously reported. The phenotype of this disorder is variable and can include hormonal resistances and severe infantile obesity with hyperphagia. A possible time relationship between the onset of obesity and endocrinopathies has been previously reported but remains unclear. Understanding of the condition’s natural history is limited, partly due to a scarcity of literature, especially in children.

Case presentation
We report three siblings with autosomal dominant PHP1B caused by a deletion in STX16 who presented with early childhood onset PTH-resistance with normocalcemia with a progressive nature, accompanied by TSH-resistance and severe infantile obesity with hyperphagia in some, not all of the affected individuals.

Conclusions
PHP1B from a STX16 deletion displays intrafamilial phenotypic variation. It is a novel cause of severe infantile obesity, which is not typically included in commercially available gene panels but must be considered in the genetic work-up. Finally, it does not seem to have a clear time relationship between the onset of obesity and hormonal resistance.