bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frontoparietal network topology as a neuromarker of music perceptual abilities
Authors: Lumaca Massimo , Keller Peter , Baggio Giosuè , Pando-Naude Victor , Bajada Claude , Martinez Mie , Hansen Josephine Hillebrand , Ravignani Andrea , Joe Nikita , Vuust Peter , Vulic Katharina , Sandberg Kristian 
Published: February 2024

Abstract

Why are some humans more musical than others? Neither fully cognitive testing nor classical localizationist neuroscience alone can provide the whole picture. Here we test how the interplay of brain organization and cognitive function delivers graded perceptual abilities in a distinctively human capacity. Our network-based study tests how human connectome variations affect music perception. We analyze multimodal magnetic resonance imaging, cognitive, and behavioral data from 200+ participants, focusing on a working memory network encompassing prefrontal and posterior parietal regions. Using graph theory, we examine structural and functional connectomes' organization in relation to musical scores. Results reveal a positive correlation between perceptual abilities and the integration efficiency of key frontoparietal regions. The linkage between functional networks and musical abilities is mediated by working memory processes, whereas structural networks influence these abilities through sensory integration. Our work lays a solid groundwork for future investigations into the neurobiological roots of musical culture(s).
Risk of stroke in patients with migraine. A register-linked HUNT study
Authors: Giri Samita , Tronvik Erling , Dalen Håvard , Ellekjær Hanne , Olsen Alexander , Hagen Knut 
Published: February 2024

Abstract
Background
The association between migraine and stroke remains unclear. The aim of this large population-based 15-year follow-up study was to investigate whether primary headache disorders, including subtypes of migraine, increase the risk of stroke.
Methods
This population-based 15-year follow-up study used baseline headache data from the third Trøndelag Health Study (HUNT3) performed between 2006 and 2008. The HUNT3 headache data were linked to the Norwegian National Stroke Register that includes stroke diagnoses recorded from 2012 until December 2021. The association between stroke and headache status was investigated in individuals aged ≥ 20 years without stroke at baseline. Prospective associations were evaluated using multivariable Cox proportional hazard models with 95% confidence intervals (CIs). Separate sub-group analyses by age and sex were performed.
Results
Among 37,364 included participants, 1,095 (2.9%) developed stroke, whereof 13.4% were younger than 55 years. In the multi-adjusted model, reporting migraine with aura (MA) at baseline was associated with increased risk of stroke at follow-up (HR 1.55, 95% CI 1.16–2.08) compared with those without headache. The increased risk of stroke was most evident among individuals with MA who were less than 55 years old (HR 1.98, 95% CI 1.20–3.27) and among women (HR 1.64, 95% CI 1.12–2.41).
Conclusions
During 15 years of follow-up, individuals with MA were more likely to suffer from stroke compared to those without headache. The relationship with MA was even stronger in women, and for young individuals aged < 55 years.
Subtractive genome mining in Xanthomonas citri pv. citri strain 306 for identifying novel drug target proteins coupled with in-depth protein-protein interaction and coevolution analysis - A leap towards prospective drug design
Authors: Mahato Tumpa , Mandal Jayanta , Chatterjee Eilita , Bhattacharya Satyabrata , Sinha Sangram 
Published: February 2024

Abstract
Citrus canker poses a serious threat to a highly significant citrus fruit crop, this disease caused by one of the most destructive bacterial plant pathogens Xanthomonas citri pv. citri (Xcc). Bacterial plant diseases significantly reduce crop yields worldwide, making it more difficult to supply the growing food demand. The high levels of antibiotic resistance in Xcc strains are diminishing the efficacy of current control measures, necessitating the exploration of novel therapeutic targets to address the escalating antimicrobial resistance trend. Genome subtraction approach along with protein-protein network and coevolution analysis were used to identify potential drug targets in Xcc stain 306. In this study we used genome mining. 750 essential proteins were identified that are nonhomologous to citrus plant. Subsequent analyses included metabolic pathway assessment, subcellular localization prediction, and druggability analysis. Protein network analysis, coevolution analysis, protein active site identification was also performed. In conclusion, this study identified eight potential drug targets, highlighting RpoN, FleQ, and SecB as unprecedented targets for Xcc. These findings may contribute to the development of novel antimicrobial agents in the future that can efficiently control citrus canker disease.
Analysis of tumorigenesis and immunological role of LTBP2: a potential predictor for immunotherapy
Authors: Wang¹ Zhenxiang , Zhang¹ Yan , Chen¹ Ying , Qian¹ Meng , Sun¹ Huihui , Xiong¹ Jie , Chen¹ Ye , Yin² Xiaomao , Tian³ Tongguan , Xu³ Yanping , Xu¹ Shuchang 
Published: February 2024

Abstract
Latent Transforming Growth Factor-β-1 Binding Protein-2 (LTBP-2), a member of the LTBP-fibrillin superfamily of extracellular matrix proteins, has been implicated in promoting TGF-β expression and secretion. However, its precise role in tumorigenesis remains ambiguous. This study comprehensively investigates the potential impact of LTBP2 on patient prognosis and response to immune therapy across various cancer types.
Method
We analyzed LTBP2 expression in tumor versus adjacent normal tissues and its correlation with survival outcomes across different cancers using the GEPIA and Sangerbox databases. The association between LTBP2 expression and tumor grade, stage, molecular subtypes, and TGF-β expression was assessed through TISIDB, Sangerbox, and TIMER2.0 databases. We also explored LTBP2's relationship with immune checkpoint (ICP) genes, tumor mutational burden (TMB), microsatellite instability (MSI), and immune cell infiltration in tumors using the Sangerbox database. Receiver Operating Characteristic (ROC) curves and Mann-Whitney tests were employed to evaluate the link between immune checkpoint blockade therapy response and LTBP2 expression. GeneMANIA and GEPIA databases were utilized for identifying LTBP2-related genes and binding proteins, while Sangerbox facilitated functional and pathway enrichment analysis.
Results
LTBP2 was differentially expressed in most cancer types compared to adjacent normal tissues. High LTBP2 expression correlated with poor prognosis in various cancers from The Cancer Genome Atlas (TCGA) dataset. Correlations were found between LTBP2 expression and TMB, MSI, ICP genes, and tumor immune cell infiltration across multiple cancer types. LTBP2 expression was positively associated with TGF-β levels and infiltration of cancer-associated fibroblasts (CAFs) in nearly all tumor types. Diverse molecular subtypes of multiple tumors also exhibited differential LTBP2 expression. ROC and Mann-Whitney tests indicated that LTBP2 could predict the outcome of immune checkpoint blockade therapy in GBMLGG, STAD, ESCA, and SKCM. Functional enrichment analysis revealed LTBP2's involvement in vascular development, cell adhesion, extracellular matrix, and TGF-β, RAS, PI3K/AKT signaling pathways in tumors.
Conclusion
Our findings indicate that LTBP2 potentially plays a significant role in tumor immunity, tumorigenesis, and treatment efficacy. LTBP2 emerges as a prospective therapeutic target and may influence the response to tumor immune checkpoint blockade therapy.
Molecular Detection of Lumpy Skin Disease Virus in Naturally Infected Cattle and Buffaloes: Unveiling the Role of Tick Vectors in Disease Spread
Authors: Zeedan Gamil SG , Abdalhamed Abeer M , Allam Ahmad M. , Abdel-Shafy Sobhy 
Published: February 2024

Abstract

Lumpy Skin Disease (LSD) is a recently pervasive viral infection affecting cattle and buffaloes in Egypt, causing considerable economic losses in the animal sector. The aim of this study is to detect the potential impact role of hard ticks, Rhipicephalus annulets (R. boophilus annulets), in the spreading of Lumpy Skin Disease Virus (LSDV), with the characterization of isolates through molecular analysis. A total of 50 skin biopsies, 110 nasal swabs, and 129 blood samples were collected from cattle of different breeds and ages across different governorates in Egypt from November 2021 to June 2022. Additionally, 145 hard ticks of R. boophilus annulatus from different stages were collected. Multiplex PCR and real-time quantitative PCR (rt-qPCR) assays using SYBR Green were targets (P32, VP32, G protein, and viral fusion protein). They detected LSDV in 17 out of 30 cattle skin biopsies 56.6%), 1 out of 7 buffalo biopsies (14.3%), and only 5 blood samples from buffaloes (11.11%). Buffaloes appeared to be less susceptible to the virus than cattle; they may act as accidental hosts. We successfully isolated the virus from hard ticks and cattle exhibiting LSD clinical signs and harboring ticks in the chorioallantois membrane (CAM) route of specific pathogen-free, embryonated chicken eggs (SPF-ECE) and confirmed the isolates by multiplex PCR and rt-qPCR. Ct values ranging from 10 to 36.5 showed the amount of LSDV-DNA in different samples. The study's findings demonstrate the widespread circulation of LSDV in both cattle and buffaloes in Egypt and provide strong evidence that hard ticks (R. annulatus) ticks play a role in LSDV transmission in susceptible animals.
Nutritional status mediates the relationship between depression and mild cognitive impairment among Chinese community-dwelling older adults
Authors: Chen Xiaoyu , Han Peipei , Liang Zhenwen , Cao Liou , Gao Jing , Tao Qiongying , Zhai Jiayi , Ding Ke , Li Weijia , Jing Biying , Yu Hongjuan , Guo Qi 
Published: February 2024

Abstract

Background: Mild cognitive impairment (MCI) and depression are prominent public health concerns among older adults, closely linked to their nutritional status. Our study aimed to explore the association and mediation pathways involving depression, nutritional status, and MCI in this population.
Methods: The study included 4799 community-dwelling Chinese older adults aged 60 years and older in Tianjin and Shanghai, China. We utilized the 30-item Geriatric Depression Scale (GDS-30) to assess depression presence and severity. A GDS-30 score ≥11 indicated the presence of depression. Participants were categorized by symptom severity: “None” (GDS-30<11), “Mild” (11≤GDS-30≤20), “Moderate to severe” (20<GDS-30≤30). Nutritional status was evaluated using the Mini Nutritional Assessment-Short Form (MNA-SF). Cognitive function was assessed with the Mini-Mental State Examination (MMSE), and daily living activities were measured using the Instrumental Activities of Daily Living (IADL). Logistic regression and mediation analyses were conducted with full adjustment for potential confounding factors.
Results: Among 4799 older adults (2010 men, mean age 71.5±5.8 years), 11.8% exhibited depression, and 12.1% had MCI, while 18.8% were at risk of malnutrition, and 1% were malnourished. Participants at risk of malnutrition or experiencing depression were associated with MCI. Additionally, nutritional status significantly mediated the relationship between depression and cognitive function, with a slightly larger mediating effect, particularly in cases of mild depression.
Conclusions: Individuals at risk of malnutrition or depressed were associated with MCI. Poor nutritional condition mediates the association between depression and MCI in older adults. Early nutritional interventions may mitigate cognitive decline in depressed older adults.
Effectiveness of tocilizumab without high-dose Glucocorticoids in patients with active Takayasu arteritis：a single center observational study
Authors: Wan Jin , Yu Zhibo , Cao Xiaoyu , Zhao Xuejian , Zhou Wei , Zheng Yi 
Published: February 2024

Abstract
Background
Current guidelines recommend high dose glucocorticoids (GC) as first-line treatment for active Takayasu arteritis (TA) and biologics including tocilizumab as second-line treatment. To minimize or avoid toxicity of GC, it will be beneficial to control TA without GC. This study is to investigate the effectiveness of tocilizumab to induce remission of active TA without synergistic application of high dose GC.
Method
The clinical data of active TA patients treated by tocilizumab in a single center were analyzed. Disease activity was assessed by NIH score and ITAS-A score. The clinical features of patients who did not achieve remission were compared with that of patients with good response.
Results
Totally 13 patients with active TA applied tocilizumab to induce remission along with methotrexate (MTX). Among them, 8 treatment-naïve patients did not use GC, 3 relapsed patients maintained 5mg/d prednisone, and 2 patients with inadequate response to conventional treatment tapered the dosage of GC. The acute reactants and TA activity scores were significantly decreased 12 weeks and 24 weeks after tocolizumab and MTX treatment. Ten patients (10/13, 76.9%) achieved remission by TCZ treatment, and most of them did not depend on the effects of GC. Three non-responders subsequently changed their treatment to high-dose GC along with immunosuppressants, but they still showed active disease 24 weeks after treatment. The prevalence of artery area pain of patients (3/3, 100%) who did not respond to tocilizumab was significantly higher than effective patients (2/10, 20%, p = 0.035). The baseline CRP and ESR level in the ineffective patients was significantly higher than that of the effective patients
Conclusion
Tocilizumab is effective to induce remission of active TA without GC in about three quarters of patients. Pain in the anatomic area of artery and high level of CRP are related to ineffectiveness of tocilizumab. Patients who resistant to tocilizumab treatment are likely refractory to conventional treatment either, and novel therapeutic strategies are required for those patients.
Which one is the best in Treating Deep Venous Thrombosis —— Percutaneous Mechanical Thrombectomy, Catheter-Directed Thrombolysis or Combination of them?
Authors: Zhang Hao , Li Xiao-ye , Li Jia-si , Xia Shi-bo , Song Chao , Lu Qing-sheng , Zhao Wei , Zhang Lei 
Published: February 2024

Abstract
Objective
To compare the treatment outcomes among percutaneous mechanical thrombectomy (PMT) with AngioJet, Catheter-directed thrombolysis (CDT), and a combination of both.
Methods
149 patients with acute or sub-acute iliac-femoral vein thrombosis accepting CDT and/or PMT were divided into three groups respectively: PMT group, CDT group, PMT + CDT group (PMT followed by CDT). The severity of thrombosis was evaluated by venographic scoring system. Technical success was defined as restored patent deep venous blood flow after CDT and/or PMT. Clinical follow-up were assessed by ultrasound or venography imaging. The primary endpoints were recurrence of DVT, and severity level of post-thrombotic syndrome (PTS) during the follow-up.
Results
Technical success and immediate clinical improvements were achieved on all patients. The proportion of sub-acute DVT and the venographic scoring in PMT + CDT group were significantly higher than that in CDT group and PMT group (proportion of sub-acute DVT: p = 0.032 and p = 0.005, respectively; venographic scoring: p < 0.001, respectively). The proportion of May-Thurner Syndrome was lower in PMT group than that in CDT and PMT + CDT group (p = 0.026 and p = 0.005, respectively). The proportion of DVT recurrence/stent thrombosis was significantly higher in CDT group than that in PMT + CDT group (p = 0.04). The severity of PTS was the highest in CDT group (χ2 = 14.459, p = 0.006) compared to PMT group (p = 0.029) and PMT + CDT group (p = 0.006).
Conclusion
Patients with sub-acute DVT, high SVS scoring and combined May-Thurner Syndrome were recommended to take PMT + CDT treatment and might have lower rate of DVT recurrence/stent thrombosis and severe PTS. Our study provided evidence detailing of PMT + CDT therapy.
Cohen syndrome due to a novel stop-gain mutation in VPS13B gene: A case report and comparative study in affected siblings worldwide
Authors: Saeediye Marziye , Parvini Farshid 
Published: February 2024

Abstract
Background
Cohen syndrome (CS) is a highly rare heterogeneous disorder associated with mutations in the vacuolar protein sorting 13 homolog B (VPS13B) gene. CS is mainly characterized by intellectual disability, microcephaly, progressive retinopathy, hypotonia, obesity, neutropenia and dysmorphic facial features.
Case presentation
: Here, we report two affected siblings suspicious to CS. Our objectives were the delineation of gene mutation causing this rare condition and comparison of observed manifestations with symptoms reported in other CS affected siblings worldwide. Whole exome sequencing (WES) in combination with Sanger sequencing were utilized to identify the disease-causing mutation in the affected siblings. Subsequently, to elucidate the effects of the mutation found, clinical data of patients were collected. Moreover, the possible impact of the identified mutation on the corresponding protein was analyzed using bioinformatics tools. A novel homozygous stop-gain mutation NM_015243: c.1043G > A: p.W348X in the VPS13B gene was identified in the proband. This mutation was confirmed by Sanger sequencing in the affected siblings and segregated with the autosomal recessive (AR) inheritance pattern of CS. Moreover, in-silico approaches highly confirmed the disease-causing nature of the identified mutation.
Conclusion
Our findings could expand the mutations spectrum of CS and the comparative study of all reported manifestations in CS affected siblings throughout the world, clearly shed light the common and uncommon manifestations of CS in different affected siblings with different VPS13B gene mutations. These results could be also useful in genetic diagnosis and counseling in CS affected patients.
The Range of Countermeasures to Reduce Inequalities in Mental Health Care for Socially Marginalized or Medically Vulnerable Adults
Authors: Zupančič Vesna , Erjavec Karmen 
Published: February 2024

Abstract

Background
Socially marginalized or medically vulnerable adults are affected by severe health inequalities and are at increased risk of poorer accessibility and quality of integrated mental health care. Health professionals often have stigmatizing attitudes towards people with mental health disorders. This study aimed to identify groups of socially marginalized or medically vulnerable adults and to collect the already implemented or proposed countermeasures to reduce inequalities in high-quality integrated mental health care for such individuals with mental health disorders.
Methods
A qualitative research approach was used for the study – a systematic literature review conducted according to PRISMA guidelines and an analysis of previous research and relevant literature on mental health care for socially marginalized and medically vulnerable adults. The time frame for the search in the respective databases ranged from 2014 to the present.
Results
This research identifies the gaps and that marginalized individuals are medically vulnerable. A spectrum of countermeasures to reduce inequalities for quality integrated mental health care for socially marginalized or medically vulnerable adults was compiled. The countermeasures need to be addressed comprehensively as part of integrated mental health and social care for marginalized or vulnerable people and promote culturally competent ethical attitudes among health professionals and in society at large.
Discussion
The countermeasures can usefully be applied to another marginalized or vulnerable group in mental health care, regardless of the level or area in which it has been identified. The range of countermeasures for individual marginalized and vulnerable groups differs only slightly, and they can usefully complement one another. The findings of this review provide a basis for a better understanding of interventions aimed at reducing stigma in health care.
Trial registration
Not applicable for a systematic review.
“Deciphering Epistatic Interactions Driving Heterosis in Brassica rapa var. Yellow Sarson"
Authors: Bisht Charu , Prasad Birendra , Pant Usha , Verma S. K. , Gaur Amit Kumar , Gupta Shubham , Panwar Neha , Joshi Sivendra , Bisht Yashpal Singh , Prasad Himanshu , Deep Harsh , Yadav Harikant 
Published: February 2024

Abstract
This study delves into the role of epistasis in elucidating heterosis manifestation in Brassica rapa var. yellow sarson hybrids. Four hybrids—YSH-0401 × Pant Sweta (C1), Pant Sweta × Apetalous (C2), PYS 2017-6 × PPS-1 (C3), and NDYS-107 × B-9 (C4)—were meticulously evaluated for seven key traits. Heightened plant stature, consistent across generations, and increased siliqua per plant with elongated siliqua length characterize the observed heterotic expression.Epistasis analysis, utilizing a six-parameter model, reveals significant additive, dominance, and epistatic interactions. Scaling tests affirm the presence of all three epistasis types (i, j, l) across diverse crosses and traits. Dominance × dominance (l) emerges as a pivotal factor, guiding heterotic expression and offering strategic breeding insights.Gene action assessment highlights patterns of duplicate gene action, complementary epistasis, and dominance variance, influencing selection strategies and breeding timelines. The correlation between heterosis and epistasis components underscores the significance of dominance × dominance in shaping superior hybrid performance. These findings contribute valuable insights to the scientific discourse on enhancing Brassica Rapa var Yellow sarson hybrid productivity, emphasizing the potential of selective mating to strategically leverage dominance and epistasis effects for optimized yields in plant breeding.