bluuebunny/crossref_metadata_2025_split huggingface dataset:
9p21 locus analysis in high‐risk gastrointestinal stromal tumors characterized for c‐kit and platelet‐derived growth factor receptor α gene alterations
Authors: Perrone Federica , Tamborini Elena , Dagrada Gian Paolo , Colombo Federica , Bonadiman Lorena , Albertini Veronica , Lagonigro Maria Stefania , Gabanti Elisa , Caramuta Stefano , Greco Angela , Torre Gabriella Della , Gronchi Alessandro , Pierotti Marco Alessandro , Pilotti Silvana 
Published: May 2005

AbstractBACKGROUNDGastrointestinal stromal tumors (GISTs) are noncomplex sarcomas that often are due to c‐kit‐activating and platelet‐derived growth factor receptor α gene (PDGFRα)‐activating mutations and perturbations of their related signaling pathways. Molecular and cytogenetic findings have indicated correlations between tumor progression and high‐risk GISTs with c‐kit mutations, the overexpression of genes such as ezrin, and losses at 9p. In particular, it was reported recently that malignant GISTs showed alterations in the p16INK4a gene located at the 9p21 locus.METHODSTo assess the involvement of p14ARF and p15INK4b in addition to p16INK4a in GISTs, the authors undertook a molecular and cytogenetic study of the 9p21 locus. A series of 22 pre‐Gleevec era, cryopreserved, high‐risk GISTs that were characterized well in terms of KIT and PDGFRα receptors were investigated for mRNA expression, homozygous deletions, mutations, and promoter methylation of locus 9p21, in some instances complemented by fluorescent in situ hybridization studies.RESULTSThe results indicated the loss of p16INK4a mRNA expression in 41% of the GISTs, mainly due to the homozygous deletion of both the p16INK4a gene and the p14ARF gene (24%). No mutations were found, and promoter methylation (detected by means of methylation‐specific polymerase chain reaction analysis in 27% of tumors) was restricted mainly to the p15INK4b gene (20%). It is noteworthy that, in all of the methylated GISTs, the epigenetic promoter alteration was coupled with mRNA expression.CONCLUSIONSAlterations in the 9p21 locus were found cumulatively in 54% of the tumors in the current series and were represented mainly by the loss of tumor suppressor gene expression. The p16INK4a deletion, which always was coupled with p14ARF gene loss, seemed to be the most common 9p21 inactivation mechanism. Cancer 2005. © 2005 American Cancer Society.
Bisphosphonate‐associated osteonecrosis of mandibular and maxillary bone
Authors: Migliorati Cesar A. , Schubert Mark M. , Peterson Douglas E. , Seneda Luis Marcelo 
Published: May 2005

AbstractBACKGROUNDThe current report presented 17 patients with cancer with bone metastases and 1 patient with osteopenia who received treatment with bisphosphonates and who subsequently developed osteonecrosis of the mandible and/or maxilla.METHODSThe authors reviewed information on 18 patients who were referred to oral medicine or oral surgery specialists for evaluation and treatment of mandibular and/or maxillary bone necrosis from June 2002 to September 2004. To be included in the current review, patients must have been treated with either pamidronate or zoledronic acid to control or prevent metastatic disease, or with alendronate for osteoporosis. All patients with cancer had received chemotherapy while receiving bisphosphonate management.RESULTSThe 17 patients with cancer were receiving active medical care for a malignancy. Cancer treatment included a variety of chemotherapeutic agents. They presented with metastatic disease to bone and were treated intravenously with the bisphosphonates pamidronate or zoledronic acid for a mean time of 25 months (range, 4–41 mos). There were 14 females and 4 males with a mean age of 62 years (range, 37–74 yrs). Malignancies included breast carcinoma (n = 10), multiple myeloma (n = 3), prostate carcinoma (n = 1), ovarian carcinoma (n = 1), prostate carcinoma/lymphoma (n = 1), and breast/ovarian carcinoma (n = 1). One female patient with osteopenia received alendronate. The most common clinical osteonecrosis presentations included infection and necrotic bone in the mandible. Associated events included dental extractions, infection, and trauma. Two patients appeared to develop disease spontaneously, without any clinical or radiographic evidence of local pathology. Despite surgical intervention, antibiotic therapy, hyperbaric oxygen therapy, and topical use of chemotherapeutic mouth rinses, most of the lesions did not respond well to therapy. Discontinuation of bisphosphonate therapy did not assure healing. However, 1 patient with cancer healed after discontinuation of bisphosphonate therapy for 4 months.CONCLUSIONSThe findings in the patient population combined with recent literature reports suggested that bisphosphonates may contribute to the pathogenesis of the oral lesions. The risk factors and precise mechanism involved in the formation of the osteonecrosis are not known. This condition represents a new oral complication in patients with cancer and can be termed bisphosphonate‐associated osteonecrosis. Lesions in patients with osteoporosis are worrisome and need to be further evaluated. Cancer 2005. © 2005 American Cancer Society.
Rapid immunohistochemistry enhances the intraoperative diagnosis of sentinel lymph node metastases in invasive lobular breast carcinoma
Authors: Leikola Junnu P. , Toivonen Terttu S. , Krogerus Leena A. , von Smitten Karl A. J. , Leidenius Marjut H. K. 
Published: May 2005

AbstractBACKGROUNDThe sensitivity of the intraoperative diagnosis of sentinel lymph node (SLN) micrometastases and the metastases of invasive lobular carcinoma (ILC) is low. The goal of the current study was to assess whether the use of intraoperative, rapid immunohistochistochemistry (IHC) enhances the intraoperative detection of micrometastases and metastases of ILC.METHODSThe sensitivity of the intraoperative diagnosis of SLN metastasis was evaluated in 438 patients when using rapid IHC with a cytokeratin biomarker. The results were compared with those obtained for 557 patients without rapid IHC but with conventional staining.RESULTSFor patients with ILC, the sensitivity of the intraoperative diagnosis was 87% (45 of 52) in the IHC group and 66% (39 of 59) in the non‐IHC group (P = 0.02). The sensitivity of the intraoperative diagnosis was similar for patients with other types of invasive cancer regardless of the use of rapid IHC. However, rapid IHC enhanced marginally the intraoperative diagnosis of the smallest micrometastases, isolated tumor cells (P = 0.06).CONCLUSIONSRapid IHC with cytokeratin labeling enhanced the intraoperative diagnosis of SLN metastases in patients with ILC. It may also improve the intraoperative diagnosis of micrometastases. Cancer 2005. © 2005 American Cancer Society.
5‐hydroxytryptamine type‐3 receptor antagonists for chemotherapy‐induced and radiotherapy‐induced nausea and emesis
Authors: Aapro Matti , Blower Peter 
Published: May 2005

AbstractBACKGROUNDNausea and emesis as a consequence of chemotherapy or radiotherapy can have an adverse effect on patients' quality of life during cancer treatment and may last for > 5 days after administration. Guidelines suggest that, used at appropriate doses, the 5‐hydroxytryptamine type‐3 (5‐HT3) receptor antagonists—which are considered the antiemetic “gold standard” when they are administered in combination with corticosteroids—demonstrate equivalent efficacy and safety. However, due to financial considerations, these agents often are used at lower doses than recommended.METHODSA literature review of relevant publications pertaining to the control of chemotherapy‐induced nausea and emesis and dosing issues of the 5‐HT3 receptor antagonists was undertaken to provide a comprehensive review of dosing issues relevant to the 5‐HT3 receptor antagonists.RESULTSThe issue of “down dosing” was particularly pertinent because of the nature of the 5‐HT3 receptor antagonist dose‐response curve: A steep dose‐response profile within a narrow dose range suggests that antiemetic control will be lost suddenly after dose deescalation. However, the array of predisposing and confounding patient factors indicates that it is unlikely that a loss of antiemetic control will be apparent across a population; rather, individuals will experience loss of control as the dose is reduced below threshold. Of the 4 5‐HT3 receptor antagonists currently licensed in the United States (granisetron, ondansetron, dolasetron, and palonosetron), ondansetron is used sometimes at lower than optimal doses, and there is evidence to suggest that even the approved oral dose of dolasetron may be suboptimal.CONCLUSIONSSuboptimal dosing not only will be detrimental to patients' quality of life but, ultimately, will prove counterproductive in terms of hospital resources, and it will add to the already significant socioeconomic burden associated with cancer therapy. Therefore, the dose of antiemetic agent administered should be sufficiently high to ensure good emesis control across the whole patient population. Cancer 2005. © 2005 American Cancer Society.
Basosquamous carcinoma
Authors: Leibovitch Igal , Huilgol Shyamala C. , Selva Dinesh , Richards Shawn , Paver Robert 
Published: May 2005

AbstractBACKGROUNDBasosquamous carcinoma (BSC) is a rare tumor defined as a basal cell carcinoma (BCC) differentiating into squamous cell carcinoma (SCC). It is reported to have a high rate of recurrence with standard wide local excision. The aim of the current study was to report a large series of patients with BSC treated with Mohs micrographic surgery (MMS).METHODSThe prospective, multicenter case series included all patients in Australia treated with MMS for BSC, who were monitored by the Skin and Cancer Foundation Australia between 1993 and 2002.RESULTSMost of the 178 tumors (95.6%) were located in the head and neck area. Recurrent tumors occurred in 47.8% of patients. The tumors were diagnosed initially as BCC in 87.4% and as SCC in 12.0% of patients. Perineural invasion was recorded in 7.9% of patients with data available. Most of these (69.0%) were previously recurrent tumors. Of 98 patients who completed a 5‐year follow‐up period after MMS, 4 (4.1%) had disease recurrence.CONCLUSIONSThe low 5‐year disease recurrence rate of BSC with MMS emphasized the importance of margin‐controlled excision using MMS. Cancer 2005. © 2005 American Cancer Society.
Preparation of an Li0.7Ni0.8Co0.2O2 Electrode Material From a New Li–Co–Ni Mixed‐Citrate Precursor
Authors: Mosqueda Yodalgis , Pérez‐Cappe Eduardo , Aranda Pilar , Ruiz‐Hitzky Eduardo 
Published: May 2005

AbstractA new chemical route to prepare the mixed oxide Li0.7Ni0.8Co0.2O2, which is a member of the well‐known Li‐Ni‐Co‐O family that is of interest as an electrode material for rechargeable lithium batteries, from a mixed citrate is reported. In a first step the new citrate (NH4)3LiNi0.8Co0.2(C6H5O7)2, is synthesized, which, after thermal decomposition, gives the layered mixed‐oxide phase Li0.7Ni0.8Co0.2O2. This oxide has been characterized by chemical analysis, XRD, FTIR, SEM, and DTA, revealing an “ideal” trigonal α‐NaFeO2 type structure with a well‐ordered distribution of Li and Ni ions in different lattices. Thermoelectric power and four‐point electrical conductivity measurements indicate a nonactivated conductivity mechanism of a small polaron. The Li0.7Ni0.8Co0.2O2 compound prepared by this so‐called “citrate route” has a better reversibility of Li‐insertion in charge–discharge cycles than related phases reported previously. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
RuIII Complexes of Edta and Dtpa Polyaminocarboxylate Analogues and Their Use as Nitric Oxide Scavengers
Authors: Storr Tim , Cameron Beth R. , Gossage Robert A. , Yee Helen , Skerlj Renato T. , Darkes Marilyn C. , Fricker Simon P. , Bridger Gary J. , Davies Nathan A. , Wilson Michael T. , Maresca Kevin P. , Zubieta Jon 
Published: May 2005

AbstractIn this study a series of RuIII complexes, chelated by analogues of ethylenediaminetetraacetic acid (edta) and diethylenetriaminepentaacetic acid (dtpa), were produced and tested for NO scavenging ability. Modifications to the edta and dtpa ligand frameworks were made in an effort to alter the reactivity, aqueous stability and pharmacokinetics of the resulting RuIII complexes. The X‐ray structure of the nitrosyl complex 38 confirms that the RuIII complex 27 reacts with NO to form a linear {Ru–NO}6 complex. The nitrosyl complex [C15H15N4O11Ru] crystallized in the P21/c space group with a = 12.731(3) Å, b = 10.894(2) Å, c = 14.241 (3) Å, β = 107.320(4)°, V = 1885.6(7) Å3, and Z = 4. Kinetic studies on the reactions of 14 (k = 2.38 × 106 M–1 s–1) and 27 (k = 2.30 × 105 M–1 s–1) with NO exemplify the difference in chemical properties obtained by ligand framework manipulation. Binding constants of 14 (KB = 5 × 106 M–1) and 27 (KB = 2 × 105 M–1) with NO were also measured, indicating the tight binding of NO by the RuIII complexes. The activity of the RuIII complexes to scavenge nitric oxide was evaluated using RAW264 murine macrophage cells. Ligand analogues of edta that have a pyridine donor as part of the N,N chelate such as 20 and 24 exhibit similar scavenging activity to the parent compound. Ligand analogues of dtpa that have R groups at the central amine in place of the carboxylic acid such as 31, 34, and 37 are also efficient NO scavengers. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Capillary electrophoresis‐laser induced fluorescence analysis of endogenous damage in mitochondrial and genomic DNA
Authors: Wirtz Michaela , Schumann Claus A. , Schellenträger Marc , Gäb Siegmar , vom Brocke Jochen , Podeschwa Michael A. L. , Altenbach Hans‐J. , Oscier David , Schmitz Oliver J. 
Published: May 2005

AbstractReactive oxygen molecules are formed in vivo as by‐products of normal aerobic metabolism. All organisms dependent on oxygen are inevitably exposed to these species so that DNA damage can occur in both genomic and mitochondrial DNA (mtDNA). In order to determine endogenous DNA damage we have developed an analytical method that involves the isolation and hydrolysis of genomic DNA or mtDNA, the labeling of modified and unmodified nucleotides and micellar electrokinetic chromatography with laser‐induced fluorescence detection. With this method we have found etheno‐adenine, thymine glycol, uracil, hypoxanthine, and 5‐methylcytosine. These were identified by the addition of internal standards to the genomic or mtDNA. There are a large number of other signals in the electropherograms of mtDNA that we have never found in genomic DNA analysis because they are at lower concentration in the genome. In the DNA of untreated patients with chronic lymphocytic leukemia (CLL), uracil and high levels of etheno‐adenine were found, which can be explained by antioxidant enzyme alterations and oxidative stress in the CLL lymphocytes.
Fractionation of glycoforms of recombinant human erythropoietin by preparative capillary isotachophoresis
Authors: Madajová Vlasta , Šimuničová Eva , Kaniansky Dušan , Marák Jozef , Zelenská Viera 
Published: May 2005

AbstractThis feasibility study deals with the use of preparative capillary isotachophoresis (CITP), operating in a discontinuous fractionation mode, to the separations and isolations of glycoforms of recombinant human erythropoietin (rhEPO). The preparative CITP separations were monitored by capillary zone electrophoresis (CZE) with a hydrodynamically closed separation unit. Such a CZE system, suppressing fluctuations of the migration data linked with fluctuations of EOF and hydrodynamic flow, made possible to evaluate and compare the preparative CITP separations performed within a longer time frame. Preparative CITP, carried out in the separation unit with coupled columns of enhanced sample loadability, separating 100 μg of rhEPO in a run lasting ca. 30 min, gave the production rate higher than 55 ng/s for the rhEPO glycoforms. The preparative separations included valve isolations of the glycoforms from the ITP stack into four or six fractions. Such numbers of the fractions corresponded to typical numbers of the major glycoform peaks as resolved in CZE of rhEPO. With respect to close effective mobilities of the glycoforms and a multicomponent nature of rhEPO, the fractions contained mixtures of glycoforms with the dominant glycoforms enriched 10–100‐fold, relative to the original rhEPO sample.
Characterization of proteins from Spirulina platensis microalga using capillary electrophoresis‐ion trap‐mass spectrometry and capillary electrophoresis‐time of flight‐mass spectrometry
Authors: Simó Carolina , Herrero Miguel , Neusüß Christian , Pelzing Matthias , Kenndler Ernst , Barbas Coral , Ibáñez Elena , Cifuentes Alejandro 
Published: May 2005

AbstractIn this work, a new capillary electrophoresis‐mass spectrometry (CE‐MS) procedure is developed to analyze proteins in Spirulina platensis microalgae. It is demonstrated that a fine optimization of several separation parameters is essential in order to achieve suitable CE‐MS analysis of these proteins in natural extracts from microalgae. Namely, optimization of the composition of the separation buffer, electrospray conditions, and washing routine between runs are required in order to obtain reliable and reproducible CE‐MS analyses of the main proteins found in this microalga (namely, allophycocyanin‐α chain, allophycocyanin‐β, c‐phycocyanin‐α, and c‐phycocyanin‐β). The relative molecular mass of these biopolymers is determined using two different MS instruments coupled to CE, i.e., CE‐ion trap‐MS and CE‐time of flight‐MS (CE‐TOF‐MS). A comparison between the results obtained with both instruments is carried out. The high resolution of the TOF‐MS enables the distinction of small modifications in proteins and, thus, a more accurate mass determination. Interestingly, molecular mass values obtained by both CE‐MS procedures agree very well while these experimental values are only in partial agreement with those theoretically expected (i.e., genetically derived masses). Some protein modifications due to amino acids exchange induced by nucleotide codon mutations are proposed to explain this difference.
Analysis of genomic DNA alterations and mRNA expression patterns in a panel of human pancreatic cancer cell lines
Authors: Gysin Stephan , Rickert Paula , Kastury Kumar , McMahon Martin 
Published: May 2005

AbstractGenomic alterations influencing the expression and/or activity of tumor suppressors or oncogenes such as KRAS2, CDKN2A, TP53, and DPC4 have been directly implicated in the initiation and progression of human pancreatic adenocarcinoma. In an effort further to systematically characterize the genomic alterations that occur in this disease, we conducted a genome wide analysis of alterations in gene copy number using array‐based comparative genomic hybridization (CGH). For this analysis, we utilized a panel of 25 human pancreatic cancer cell lines derived from either primary or metastatic tumors. This panel also included a metastatic progression series of cell lines derived from COLO 357 cells. Array CGH permitted the identification of alterations in the copy number of genes that might participate in the aberrant behavior of pancreatic cancer cells. In addition, the acquisition of invasive and metastatic potential by derivatives of COLO 357 cells was accompanied by additional focal genomic alterations including point mutations and amplification of KRAS2. To complement the array CGH analysis, we also conducted an analysis of mRNA expression patterns in a subset of these cells using cDNA microarrays. By this means, we identified a set of candidate genes, including those regulated by RAS signaling, that may contribute to the process of cancer cell invasion and metastasis. Supplementary material for this article can be found on the Genes, Chromosomes, and Cancer website at http://www.interscience.wiley.com/jpages/1045–2257/suppmat/index.html. © 2005 Wiley‐Liss, Inc.
Cerebral mechanisms of prosodic sensory integration using low‐frequency bands of connected speech
Authors: Hesling Isabelle , Dilharreguy Bixente , Clément Sylvain , Bordessoules Martine , Allard Michèle 
Published: May 2005

AbstractEven if speech perception has been reported to involve both left and right hemispheres, converging data have posited the existence of a functional asymmetry at the level of secondary auditory cortices. Using fMRI in 12 right‐handed French men listening passively to long connected speech stimuli, we addressed the question of neuronal networks involved in the integration of low frequency bands of speech by comparing 1) differences in brain activity in two listening conditions (FN, NF) differing in the integration of pitch modulations (in FN, low frequencies, obtained by a low‐pass filter, are addressed to the left ear while the whole acoustic message is simultaneously addressed to the right ear, NF being the reverse position); 2) differences in brain activity induced by high and low degrees of prosodic expression (expressive vs. flat); and 3) effects of the same connected speech stimulus in the two listening conditions. Each stimulus induced a specific cerebral network, the flat one weakening activations which were mainly reduced to the bilateral STG for both listening conditions. In the expressive condition, the specific sensory integration FN results in an increase of the articulatory loop and new recruitments such as right BA6‐44, left BA39‐40, the left posterior insula and the bilateral BA30. This finding may be accounted for by the existence of temporal windows differing both in length and in acoustic cues decoding, strengthening the “asymmetric sampling in time” hypothesis posited by Poeppel (Speech Commun 2003; 41:245–255). Such an improvement of prosodic integration could find applications in the rehabilitation of some speech disturbances. Hum Brain Mapp, 2005. © 2005 Wiley‐Liss, Inc.
Parametric analysis of oscillatory activity as measured with EEG/MEG
Authors: Kiebel Stefan J. , Tallon‐Baudry Catherine , Friston Karl J. 
Published: May 2005

AbstractWe assess the suitability of conventional parametric statistics for analyzing oscillatory activity, as measured with electroencephalography/magnetoencephalography (EEG/MEG). The approach we consider is based on narrow‐band power time–frequency decompositions of single‐trial data. The ensuing power measures have a χ2‐distribution. The use of the general linear model (GLM) under normal error assumptions is, therefore, difficult to motivate for these data. This is unfortunate because the GLM plays a central role in classical inference and is the standard estimation and inference framework for neuroimaging data. The key contribution of this work is to show that, in many circumstances, one can appeal to the central limit theorem and assume normality for generative models of power. If this is not appropriate, one can transform the data to render the error terms approximately normal. These considerations allow one to analyze induced and evoked oscillations using standard frameworks like statistical parametric mapping. We establish the validity of parametric tests using synthetic and real data and compare its performance to established nonparametric procedures. Hum Brain Mapp, 2005. © 2005 Wiley‐Liss, Inc.