bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Clinical Evaluation of a Highly Sensitive DNA Microarray for Detection and Genotyping of Human Papillomaviruses
Authors: Oh Tae Jeong , Kim Chang Jin , Woo Suk Kyung , Kim Tae Seung , Jeong Dong Jun , Kim Myung Soon , Lee Sunwoo , Cho Hyun Sill , An Sungwhan 
Published: July 2004

ABSTRACT
Human papillomavirus (HPV) has been found in cervical cancer, tonsillar cancer, and certain types of head and neck cancers. We report on a DNA microarray-based method for the simultaneous detection and typing of HPVs. The genotype spectrum discriminated by this HPV DNA microarray includes 15 high-risk HPV genotypes and 12 low-risk HPV genotypes. The HPV DNA microarray showed high degrees of specificity and reproducibility. We evaluated the performance of the HPV DNA microarray by application to three HPV-positive cell lines (HeLa, Caski, and SiHa cells) and two HPV-negative cell lines (C33A and A549 cells). The HPV DNA microarray successfully identified the known types of HPV present in the cell lines. The detection limit of the HPV DNA microarray was at least 100-fold higher than that of PCR. To assess the clinical applicability of the HPV DNA microarray, we performed the HPV genotyping assay with 73 nonmalignant and malignant samples from 39 tonsillar cancer patients. Twenty-five of the 39 (64.1%) malignant samples were positive for HPV, whereas 3 of 34 (8.8%) nonmalignant samples were positive for HPV. This result shows a preferential association of HPV with tonsillar carcinomas. The correlations of the presence of HPV with the grade of differentiation and risk factors were not significant. Our data show that the HPV DNA microarray may be useful for the diagnosis and typing of HPV in large-scale epidemiological studies.
Evaluation of a Real-Time PCR Assay Using the LightCycler System for Detection of Parvovirus B19 DNA
Authors: Buller Richard S. , Storch Gregory 
Published: July 2004

ABSTRACT
We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.
Epidemiological Investigation of Bloodstream Infections by Extended Spectrum Cephalosporin-ResistantEscherichia coliin a Taiwanese Teaching Hospital
Authors: Yan Jing-Jou , Ko Wen-Chien , Wu Jiunn-Jong , Tsai Shu-Huei , Chuang Chin-Luan 
Published: July 2004

ABSTRACTIn an epidemiologic and case-control study including 30 case patients over a 3.5-year period in a Taiwanese university hospital, only β-lactamase inhibitor use and extended-spectrum cephalosporin use were identified as independent risk factors for nosocomial CMY-2-producingEscherichia colibloodstream infection, and CMY-2 producers were found more prevalent than extended-spectrum β-lactamase-producing isolates.
Fungemia Caused by
Zygoascus hellenicus
in an Allogeneic Stem Cell Transplant Recipient
Authors: Brandt Mary E. , Kauffman Carol A. , Pappas Peter G. , Iqbal Naureen , Arthington-Skaggs Beth A. , Lee-Yang Wendy , Smith Maudy T. 
Published: July 2004

ABSTRACT

Zygoascus hellenicus
(
Candida hellenica
) was isolated from a blood culture from a patient who had received an allogeneic stem cell transplant. The isolate displayed an antifungal susceptibility pattern of decreased susceptibility to fluconazole and itraconazole, high susceptibility to voriconazole, and low susceptibility to caspofungin. The organism was misidentified by a commercial yeast identification system. This is the first reported case of human infection with this rare ascomycetous yeast.
dx2-y2-STATE OF HIGH TEMPERATURE SUPERCONDUCTORS WITH A SMALL ADMIXTURE OF dxy-STATE
Authors: LEE C. Y. 
Published: July 2004

It is well known that most of the high temperature superconductors (at least hole-type) are in d-wave state. But it is still an unsolved problem whether it is a pure d-wave state or one has some kind of mixed state. Among the candidates for an admixture, there are s- and d-wave states. Existing experiments could not resolve this issue. New possibilities for experimental resolution of this problem are opened via recent observation of the collective modes in UBe 13 (heavy fermion superconductor) by microwave impedance technique experiments and in Sr 2 RuO 4 (high temperature superconductor) by ultrasound attenuation experiments. Some theoretical treatments show that the most likely state is a mixture of two d-wave states: dx2-y2 and dxy with a small admixture of former state. To create the theoretical basis for investigation of possible mixed superconducting state in unconventional superconductors by sound attenuation and microwave absorption experiments, I derive for the first time a full set of equations for collective modes spectrum in dx2-y2-state with small admixture of dxy state. These equations allow to calculate the whole collective mode spectrum in mixed dx2-y2+iεdxy state and distinguish this state from pure d-wave states (whose collective mode spectrum has been calculated earlier) by ultrasound attenuation and microwave absorption experiments.
National Cultural Differences in the Use and Perception of Internet-based NSS: Does High or Low Context Matter?
Authors: Kersten Gregory , Vetschera Rudolf , Koeszegi Sabine 
Published: July 2004

AbstractIn this article, we apply an extended technology acceptance model (TAM) to explore whether national culture influences a user's perception and use of Internet-based negotiation support systems (NSS). In particular, we are interested in whether different preferences for communication patterns, as we find them for low-context and high-context cultures, influence the use and perception of different NSS support tools. The Web-based system Inspire, which provided data for our analysis has been used by over 2000 entities worldwide in experimental negotiations. Our results show that, based on the need to establish a social context in computermediated negotiations, users from high-context cultures exchange significantly more messages and offers during negotiations than users from low-context cultures. Analytical negotiation support is valued significantly higher by users from low-context cultures than by users from highcontext cultures as this problem-solving approach is more compatible with their preference for direct and task-oriented communication.
Design and Optimisation of an RTM Composite Bicycle Crank
Authors: Quaresimin M. , Meneghetti G. , Verardo F. 
Published: July 2004

This paper presents the activities performed for the design and optimisation of a new carbon fibre composite bicycle crank produced by using the RTM technology. A prototype of the right crank was developed on the basis of a preliminary design supported by a finite element model. Its flexural and torsional stiffness performances were experimentally evaluated and compared with those of top quality commercial cranks showing the need for an improvement of the composite prototype stiffness. The FE model was then calibrated on the basis of the constituent material characterisation and the definition of suitable boundary conditions in order to achieve a reliable simulation of the experimental tests. Finally, an FE optimisation was performed to increase the crank stiffness and to investigate the influence of the elastic properties and lay-up of the composite skin on the stiffness performances.
Evaluation of different methods of leukoreduction of donor platelets to prevent alloimmune platelet refractoriness and induce tolerance in a canine transfusion model
Authors: Slichter Sherrill J. , Fish Douglas , Abrams V. Kraig , Gaur Lakshmi , Nelson Karen , Bolgiano Doug 
Published: July 2004

AbstractThe effectiveness of different methods of leukoreduction in preventing alloimmune platelet refractoriness was evaluated in a canine model. Platelets from a random donor dog were administered for up to 8 weeks or until platelet refractoriness. Standard (STD; unmodified) platelets were accepted by 14% of recipients (n = 7) compared with 14% for centrifuge leukoreduced (C-LR) platelets (n = 21) and 31% for filter leukoreduced (F-LR) platelets (n = 13; no significant differences). Surprisingly, using both F-LR and C-LR platelets was highly effective (87% acceptance, n = 15). Transfusing F-LR/C-LR red blood cells (n = 4) or F-LR/C-LR plasma (n = 4), along with F-LR/C-LR platelets, did not affect platelet acceptance (100% acceptance). Overall acceptance of F-LR/C-LR platelets was 91% (n = 23; P ≤ .05 versus STD, C-LR, or F-LR platelets). F-LR/C-LR transfusions also induced tolerance to subsequent STD platelet transfusions from the same donor (82% acceptance, n = 19) as well as to donor skin grafts without recipient immunosuppression (57% acceptance, n = 7). To evaluate mechanisms of tolerance induction, F-LR/C-LR platelets were γ-irradiated. Although the γ-irradiated F-LR/C-LR platelets were uniformly accepted (n = 6), tolerance to STD platelets was lost. These data suggest that some allostimulatory white cells are filter adherent, whereas others escape filtration but can be removed by centrifugation and tolerance requires a residual functioning white cell.
Glutathione-S-transferase π inhibits As2O3-induced apoptosis in lymphoma cells: involvement of hydrogen peroxide catabolism
Authors: Zhou Li , Jing Yongkui , Styblo Miroslav , Chen Zhu , Waxman Samuel 
Published: July 2004

AbstractArsenic trioxide (As2O3) is an effective agent for the treatment of relapsed and refractory acute promyelocytic leukemia by induction of partial differentiation and apoptosis. As2O3, at therapeutic concentrations (1-2 μM), induced apoptosis in Raji lymphoma cells but not in Jurkat lymphoma cells, which inversely correlated with the levels of glutathione-S-transferase π (GSTP1), but not GSTπ1 and GSTM1, expression and activity. GSTP1 mRNA, protein level, and activity were high in Jurkat cells but undetectable in Raji cells. Stable transfection of GSTP1 into Raji cells decreased the amount of As2O3-induced apoptosis. Apoptosis induced by therapeutic concentrations of As2O3 in Raji cells is related to increasing H2O2 intracellular accumulation but not to JNK activation. Forced expression of GSTP1 by transfection of Raji cells significantly decreased the basal amount of H2O2 and its levels after therapeutic concentration of As2O3 treatment. Added exogenous H2O2 was removed more rapidly, which correlated with a greater decrease in reduced glutathione level in Raji clones expressing GSTP1 than in those clones without GSTP1 expression. Overexpression of GSTP1 in transfected Raji clones was also found to decrease the retention of As2O3. These data suggest that GSTP1 blocks As2O3-induced apoptosis in lymphoma cells by decreasing intracellular amounts of H2O2 by catabolism and H2O2 production by decreasing the intracellular retention of As2O3.
Human CD62L– memory T cells are less responsive to alloantigen stimulation than CD62L+ naive T cells: potential for adoptive immunotherapy and allodepletion
Authors: Foster Aaron E. , Marangolo Marina , Sartor Mary M. , Alexander Stephen I. , Hu Min , Bradstock Kenneth F. , Gottlieb David J. 
Published: July 2004

AbstractSelective depletion of alloreactive T cells from allogeneic stem cell grafts can reduce graft-versus-host disease (GVHD) while preserving beneficial effects of T cells including facilitation of engraftment, protection against opportunistic infection, and reduced relapse risk. Memory T cells (CD62L–) represent a population of T cells that have previously encountered pathogens and may contain fewer T cells capable of recognizing neoantigens including recipient allogeneic antigen (aAg). We investigated whether human naive (CD62L+) or memory (CD62L–) T cells had different capacities to respond to aAg by assessing their ability to proliferate in response to and lyse HLA-mismatched Epstein-Barr virus–transformed B cells. Freshly sorted and in vitro expanded CD62L– memory T cells were less responsive to aAg stimulation than were CD62L+ naive T cells but contained higher levels of cytomegalovirus (CMV)–specific T cells. Analysis of T cell receptor (TCR) repertoire showed restricted TCR diversity in the memory T-cell population possibly due to selection associated with chronic exposure to common pathogens. Memory T cells may represent a donor cell subpopulation suitable for enhancing immune reconstitution without increasing the risk of GVHD.
Constitutively active Notch4 promotes early human hematopoietic progenitor cell maintenance while inhibiting differentiation and causes lymphoid abnormalities in vivo
Authors: Vercauteren Suzanne M. , Sutherland Heather J. 
Published: July 2004

AbstractNotch transmembrane receptors are known to play a critical role in cell-fate decisions, with Notch1 shown to enhance self-renewal of hematopoietic stem cells and cause T-cell leukemia. Four Notch receptors exist, and the extent of redundancy and overlap in their function is unknown. Notch4 is structurally distinct from Notch1 through Notch3 and has not been extensively studied in hematopoiesis. By polymerase chain reaction (PCR) we find Notch4 transcript expression in human marrow cells and in both CD34+ and CD34– populations. When constitutively active Notch1 or Notch4 was overexpressed in normal human marrow or cord cells, we found reduced colony-forming and short-term proliferative ability while the primitive progenitor content of myeloid long-term cultures was significantly increased. Notch4–intracellular domain (Notch4-IC)–transduced cord cells transplanted into β2-microglobulin–/– nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice resulted in significantly higher levels of engraftment of both green fluorescent protein–positive (GFP+) and GFP– populations as compared with controls. GFP+ cells in bone marrow and spleen of animals that had received transplants gave rise to an immature CD4+CD8+ T-cell population, whereas B-cell development was blocked. These results indicate that activation of Notch4 results in enhanced stem cell activity, reduced differentiation, and altered lymphoid development, suggesting it may influence both stem cells and the fate of the common lymphoid progenitor.
Formation of human hepatocytes by human hematopoietic stem cells in sheep
Authors: Almeida-Porada Graça , Porada Christopher D. , Chamberlain Jason , Torabi Ali , Zanjani Esmail D. 
Published: July 2004

AbstractWe took advantage of the proliferative and permissive environment of the developing preimmune fetus to develop a noninjury large animal model in sheep, in which the transplantation of defined populations of human hematopoietic stem cells resulted in the establishment of human hematopoiesis and led to the formation of significant numbers of long-lasting, functional human liver cells, with some animals exhibiting levels as high as 20% of donor (human) hepatocytes 11 months after transplantation. A direct correlation was found between hepatocyte activity and phenotype of transplanted cells, cell dose administered, source of cells used on a cell-per-cell basis (bone marrow, cord blood, mobilized peripheral blood), and time after transplantation. Human hepatocytes generated in this model retained functional properties of normal hepatocytes, constituted hepatic functional units with the presence of human endothelial and biliary duct cells, and secreted human albumin that was detected in circulation. Transplanting populations of hematopoietic stem cells can efficiently generate significant numbers of functional hepatic cells in this noninjury large animal model and thus could be a means of ameliorating or curing genetic diseases in which a deficiency of liver cells or their products threatens the life of the fetus or newborn.
Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells
Authors: Giebel Bernd , Corbeil Denis , Beckmann Julia , Höhn Johannes , Freund Daniel , Giesen Kay , Fischer Johannes , Kögler Gesine , Wernet Peter 
Published: July 2004

AbstractDuring ontogenesis and the entire adult life hematopoietic stem and progenitor cells have the capability to migrate. In comparison to the process of peripheral leukocyte migration in inflammatory responses, the molecular and cellular mechanisms governing the migration of these cells remain poorly understood. A common feature of migrating cells is that they need to become polarized before they migrate. Here we have investigated the issue of cell polarity of hematopoietic stem/progenitor cells in detail. We found that human CD34+ hematopoietic cells (1) acquire a polarized cell shape upon cultivation, with the formation of a leading edge at the front pole and a uropod at the rear pole; (2) exhibit an amoeboid movement, which is similar to the one described for migrating peripheral leukocytes; and (3) redistribute several lipid raft markers including cholesterol-binding protein prominin-1 (CD133) in specialized plasma membrane domains. Furthermore, polarization of CD34+ cells is stimulated by early acting cytokines and requires the activity of phosphoinositol-3-kinase as previously reported for peripheral leukocyte polarization. Together, our data reveal a strong correlation between polarization and migration of peripheral leukocytes and hematopoietic stem/progenitor cells and suggest that they are governed by similar mechanisms.
Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues
Authors: Murdoch Craig , Giannoudis Athina , Lewis Claire E. 
Published: July 2004

AbstractThe mechanisms responsible for recruiting monocytes from the bloodstream into solid tumors are now well characterized. However, recent evidence has shown that these cells then differentiate into macrophages and accumulate in large numbers in avascular and necrotic areas where they are exposed to hypoxia. This parallels their tendency to congregate in ischemic areas of other diseased tissues such as atherosclerotic plaques and arthritic joints. In tumors, macrophages appear to undergo marked phenotypic changes when exposed to hypoxia and to switch on their expression of a number of mitogenic and proangiogenic cytokines and enzymes. This then promotes tumor growth, angiogenesis, and metastasis. Here, we compare the various mechanisms responsible for monocyte recruitment into tumors with those regulating the accumulation of macrophages in hypoxic/necrotic areas. Because the latter are best characterized in human tumors, we focus mainly on these but also discuss their relevance to macrophage migration in ischemic areas of other diseased tissues. Finally, we discuss the relevance of these mechanisms to the development of novel cancer therapies, both in providing targets to reduce the proangiogenic contribution made by hypoxic macrophages in tumors and in developing the use of macrophages to deliver therapeutic gene constructs to hypoxic areas of diseased tissues.
Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions
Authors: Zhang Wen Jie , Park Changwon , Arentson Elizabeth , Choi Kyunghee 
Published: July 2004

AbstractEmbryonic stem (ES) cells can differentiate into many different somatic cells in culture. To better correlate hematopoietic and endothelial cell differentiation of ES cells in currently available protocols, we compared fetal liver kinase-1 (Flk-1)–, stem cell leukemia (Scl)–, and vascular endothelial–cadherin (VE-cadherin)–expressing cells generated in embryoid bodies (EBs) and on OP9 cells. We report that the kinetics of Scl and Flk-1 expression were similar in EBs and OP9 cells, although Flk-1 expression was extended on OP9 cells. CD45+ and Ter-119+ cells developed more efficiently in EBs, whereas VE-cadherin+ cells developed largely on OP9 cells. Cell sorting and replating studies showed that Scl+ cells, not Flk-1+ or VE-cadherin+ cells, were enriched for primitive and definitive hematopoietic progenitors. Our studies indicate that optimal hematopoietic and endothelial cell differentiation occur in EBs and on OP9 cells, respectively. Regardless of the culture systems used, Scl is the most relevant marker for enriching primitive and definitive hematopoietic progenitors.
Control of Phosphatase and Tensin Homolog (PTEN) Gene Expression in Normal and Neoplastic Thyroid Cells
Authors: Tell Gianluca , Pines Alex , Arturi Franco , Cesaratto Laura , Adamson Eileen , Puppin Cinzia , Presta Ivan , Russo Diego , Filetti Sebastiano , Damante Giuseppe 
Published: July 2004

Abstract
The lipid phosphatase, phosphatase and tensin homolog (PTEN), is a key element in controlling cell growth and survival and has a well established role as tumor suppressor protein in many neoplasia. Several data indicate that silencing of PTEN gene expression may be relevant in follicular thyroid cell transformation. Thus, in the present study regulation of PTEN gene expression in thyroid cells was investigated. Cotransfection experiments indicated that in normal FRTL-5 rat thyroid cells, PTEN promoter activity was increased by overexpression of the transcription factor early growth response protein-1 (Egr-1). Moreover, Western blot experiments indicated that when Egr-1 expression was up-regulated by treating FRTL-5 cells with H2O2, an increase in PTEN expression was also observed. TSH induced opposite modifications on PTEN and Egr-1 protein levels. Moreover, acute or chronic TSH stimulation determined distinct effects. In fact, acute TSH stimulation (30 and 60 min) induced a decrease in PTEN, but an increase in Egr-1 protein levels. These effects were cAMP dependent; in fact, they were mimicked by forskolin. A chronic TSH treatment (5 d) stimulated PTEN protein expression, whereas Egr-1 protein was down-regulated. In contrast to normal thyroid cells, when the thyroid tumor cell lines ARO and BCPAP were exposed to H2O2, neither Egr-1 nor PTEN protein levels were increased. Acute stimulation of ARO and BCPAP cells with forskolin increased Egr-1, but not PTEN, protein levels. Therefore, thyroid tumor cell lines show alteration of PTEN gene expression regulation. RT-PCR experiments performed on human thyroid tumors showed that the absence of Egr-1 mRNA is always paralleled by the absence of PTEN mRNA. Thus, modification of the Egr-1-dependent mechanisms may play a role in the silencing of PTEN gene expression occurring during thyroid cell transformation.