bluuebunny/crossref_metadata_2025_split huggingface dataset:
What can Drosophila tell us about serpins, thrombosis and dementia?
Authors: Carrell Robin , Corral Javier 
Published: December 2003

AbstractThe validity of the fruit‐fly as a model of human disease has been confirmed in a striking way by Green and colleagues.1 They show that the mutations causing a necrotic disease phenotype in Drosophila, precisely mirror those resulting in a group of well‐studied but perplexing diseases in the human. These diseases, ranging from thrombosis to dementia, arise from mutations causing a conformational instability of serpin protease inhibitors. The findings provide clues as to the unusual severity and variable onset of such conformational diseases and demonstrate the potential of Drosophila as a model for their future study. BioEssays 26:1–5, 2004. © 2003 Wiley Periodicals, Inc.
Reassessing the molecular biology of sperm–egg recognition with mouse genetics
Authors: Dean Jurrien 
Published: December 2003

AbstractThe zona pellucida is an extracellular coat that surrounds mammalian eggs and early embryos. This insoluble matrix separates germ from somatic cells during folliculogenesis and plays critical roles during fertilization and early development. The mouse and human zona pellucida contain three glycoproteins (ZP1 or ZPB, ZP2, ZP3), the primary structures of which have been deduced by molecular cloning. Targeted mutagenesis of endogenous mouse genes and transgenesis with human homologues provide models to investigate the roles of individual zona components. Collectively, the genetic data indicate that no single mouse zona pellucida protein is obligatory for taxon‐specific sperm binding and that two human proteins are not sufficient to support human sperm binding. An observed post‐fertilization persistence of mouse sperm binding to “humanized” zona pellucida correlates with uncleaved ZP2. These observations are consistent with a model for sperm binding in which the supramolecular structure of the zona pellucida necessary for sperm binding is modulated by the cleavage status of ZP2. BioEssays 26:29–38, 2004. Published 2003 Wiley Periodicals, Inc.
Cross‐strand disulphides in cell entry proteins: poised to act
Authors: Wouters Merridee A. , Lau Ken K. , Hogg Philip J. 
Published: December 2003

AbstractCross‐strand disulphides (CSDs) are unusual bonds that link adjacent strands in the same β‐sheet. Their peculiarity relates to the high potential energy stored in these bonds, both as torsional energy in the highly strained disulphide linkage and as deformation energy stored in the sheet itself. CSDs are relatively rare in protein structures but are conspicuous by their presence in proteins that are involved in cell entry. The finding that entry of botulinum neurotoxin and HIV into mammalian cells involves cleavage of CSDs suggests that the activity of other cell entry proteins may likewise involve cleavage of these bonds. We examine emerging evidence of the involvement of these unusual disulphides in cell entry events. BioEssays 26:73–79, 2004. © 2003 Wiley Periodicals, Inc.
Preparation of nanoparticles by electrocoagulation from soluble exopolysaccharide produced by Claviceps viridis
Authors: Flieger M. , Kantorová M. , Benada O. , Kofroňová O. , Sobotka M. , Cvak J. , Votruba J. 
Published: December 2003

AbstractElectrocoagulation is an evolving technology that has been effectively applied for wastewater treatment but its applications in biotechnology and nanotechnology are very limited. This method was applied for the preparation of nanoparticles from soluble exopolysaccharide (EPS) produced by Claviceps viridis in a submerged batch culture. A cathode/anode pair electrode (Al or Fe) system was used for determination of the separation rates of electrocoagulation and the yields of EPS nanoparticles production. The separation rates of 0.170 ± 0.003 mg EPS/sec (Fe electrodes) and 0.250 ± 0.003 mg EPS/sec (Al electrodes) were calculated for voltage gradient 1 V/1 cm of electrodes distance and were constant during experiments. The specific yield of EPS nanoparticles production based on the consumed electric power was dependent on the material of the electrodes and its value was determined as 0.71 ± 0.01 mg EPS/W for Fe electrodes and 0.91 ± 0.01 mg EPS/W for Al electrodes, respectively. © 2003 Wiley Periodicals, Inc.
Percutaneous ulnar artery approach for primary coronary angioplasty: Safety and feasibility
Authors: Limbruno Ugo , Rossini Roberta , De Carlo Marco , Amoroso Giovanni , Ciabatti Nicola , Petronio Anna Sonia , Micheli Andrea , Mariani Mario 
Published: December 2003

AbstractTransradial approach in primary and rescue angioplasty may be advantageous with respect to the femoral access due to the lower incidence of vascular complications. Ulnar cannulation has been proposed for elective procedures in patients not suitable for transradial approach. We here report on 13 patients undergoing primary angioplasty performed using the transulnar approach. Ulnar access was finally obtained in 10 patients, sheath insertion time ranged from 2 to 5 min, time from arterial puncture to vessel recanalization ranged from 21 to 36 min. Primary angioplasty was successful in all patients. At 30‐day echo color Doppler, all ulnar arteries were patent and with a physiologic pattern of flow. Subcutaneous hemorrhage of the forearm was observed in two patients, whereas hematoma, pseudoaneurysm, thrombus, and arterovenous fistula were not observed. In conclusion, transulnar access may represent an additional option in patients undergoing primary angioplasty when the radial artery access site is not available. Catheter Cardiovasc Interv 2004;61:56–59. © 2004 Wiley‐Liss, Inc.
Assessment of anticoagulation using activated clotting times in patients receiving intravenous enoxaparin during percutaneous coronary intervention
Authors: Lawrence Mark , Mixon Timothy A. , Cross Donald , Gantt D. Scott , Dehmer Gregory J. 
Published: December 2003

AbstractEnoxaparin is being used more frequently in patients undergoing percutaneous coronary intervention (PCI). In this study, we determined the effect of intravenous enoxaparin on activated clotting time (ACT) measurements in the setting of PCI. In 67 consecutive patients, either 1 mg/kg intravenous enoxaparin alone was given for anticoagulation or 0.75 mg/kg given in patients receiving eptifibatide. ACT was measured before and 5 min following enoxaparin administration. After 1 mg/kg enoxaparin (n = 22), mean ACT increased from 122 ± 22 to 199 ± 20 sec. After 0.75 mg/kg enoxaparin and eptifibatide (n = 45), mean ACT increased from 125 ± 22 to 194 ± 24 sec. The mean increase in ACT was 77 ± 26 sec in the 1 mg/kg group and 69 ± 23 sec in the 0.75 mg/kg group (both P values < 0.0001). Moreover, in a subgroup of 26 patients, there was an excellent correlation (r = 0.86) between ACTs and the ENOX test, a new point‐of‐care test for assessing enoxaparin anticoagulation. None of the patients had transient abrupt closure, thrombus formation, major bleeding, or required urgent revascularization. Intravenous enoxaparin at clinically relevant doses with and without eptifibatide increases ACT levels at 5 min in patients undergoing PCI. These data suggest the ACT may be useful in the assessment of anticoagulation by enoxaparin. Catheter Cardiovasc Interv 2004;61:52–55. © 2004 Wiley‐Liss, Inc.
Retrospective image‐based gating of intracoronary ultrasound images for improved quantitative analysis: The intelligate method
Authors: de Winter Sebastiaan A. , Hamers Ronald , Degertekin Muzzafer , Tanabe Kengo , Lemos Pedro A. , Serruys Patrick W. , Roelandt Jos R.T.C. , Bruining Nico 
Published: December 2003

AbstractQuantitative analysis of intracoronary ultrasound (ICUS) studies is performed on a series of tomographic cross‐sectional ICUS images acquired during a motorized 0.5 mm/sec catheter pullback. Catheter displacement in the vascular lumen during the cardiac cycle causes an anatomically shuffled ICUS study, which results in a sawtooth‐shaped appearance of the coronary segment in longitudinal reconstructed views in quantitative coronary ultrasound software packages. This hampers contour detection and leads to a laborious time‐consuming semiquantitative analysis process that may produce inaccurate results. To solve these problems, in the past, online ECG‐gated acquisition hardware has been applied. This article describes a novel image‐based gating method called Intelligate, which features automatic retrospective selection of end‐diastolic frames from videotaped or digitally stored ICUS studies. Our evaluation shows that there are no quantitative differences between analysis results of hardware ECG‐gated and Intelligated ICUS studies. Catheter Cardiovasc Interv 2004;61:84–94. © 2004 Wiley‐Liss, Inc.
Management of iatrogenic radial artery perforation
Authors: Calviño‐Santos Ramón Antonio , Vázquez‐Rodríguez José Manuel , Salgado‐Fernández Jorge , Vázquez‐González Nicolás , Pérez‐Fernández Ruth , Vázquez‐Rey Eugenia , Castro‐Beiras Alfonso 
Published: December 2003

AbstractThe aim of this study was to evaluate a new protocol allowing coronary angiography to be performed transradially in spite of the occurrence of iatrogenic radial artery perforation during catheterization. Nine patients with iatrogenic radial artery perforation were managed conservatively by inserting a long arterial sheath in the damaged radial artery up to the brachial artery, after which the diagnostic and/or interventional procedures that had motivated transradial catheterization were completed via the protected radial artery. Radial angiography performed immediately thereafter showed no extravasation, and no major vascular complications developed during follow‐up. The day after the procedure, two patients had asymptomatic radial occlusion, but the other seven patients had normal radial pulses and reversed Allen test responses showing normal perfusion. A conservative management technique, installation of a long arterial sheath not only promotes resolution of iatrogenic radial artery perforation but also allows the procedures motivating catheterization to be completed transradially. Catheter Cardiovasc Interv 2004;61:74–78. © 2004 Wiley‐Liss, Inc.
Outcomes and alternative techniques for device closure of the large secundum atrial septal defect
Authors: Varma Chetan , Benson Lee N. , Silversides Candice , Yip J. , Warr Matthew R. , Webb Gary , Siu Samuel C. , McLaughlin Peter R. 
Published: December 2003

AbstractOutcomes of device closure of large and small secundum atrial septal defects (ASDs) as related to rim anatomy with the Amplatzer atrial septal occluder were compared. Rim adequacy (≥ 5mm) of the anterior, inferior, posterior, and superior rims was determined using transesophageal echocardiography. Balloon‐stretched defect size defined patients into two groups: group 1, ≤ 25 mm (n = 138); group 2, > 25 mm (n = 34). Rim deficiency (n = 62) was more frequent in group 2 compared to group 1 (50% vs. 33%; P = 0.07), especially inferior rim deficiency (35% vs. 2%; P = 0.005). Device deployment was successful in group 1 and group 2 (100% vs. 91%; P = 0.007). Unsuccessful deployment was associated with an ASD of > 25 mm (P = 0.007) and inferior rim deficiency (P = 0.001). At first follow‐up (54 ± 16 days), right ventricular systolic pressure had improved in both groups (P < 0.001). Closure of a large ASD associated with a lack of support in the inferior rim may warrant alternative strategies to position the device successfully. Catheter Cardiovasc Interv 2004;61:131–139. © 2004 Wiley‐Liss, Inc.
Damage to a covered stent by a thrombectomy device
Authors: Pate Gordon , Carere Ronald , Webb John 
Published: December 2003

AbstractA 75‐year‐old with a history of coronary bypass grafting was found to have significant stenoses in a vein graft to the right coronary artery. Symbiot stents, with a polytetrafluoroethylene covering, were used to treat areas in the proximal and middle sections of the graft. The following day, she developed symptoms and signs consistent with an acute inferior infarction. The graft was found to be thrombosed. Thrombectomy was performed using an X‐Sizer device, consisting of a helical cutter connected to a vacuum source. This resulted in damage to the stent lining, which led to jamming of the device and subsequent removal of a piece of the stent covering. Treatment was by way of deployment of a long stent within the damaged stent. Recovery was unremarkable. Caution or avoidance of this device appears warranted in the setting of covered stent procedures. Catheter Cardiovasc Interv 2004;61:107–110. © 2004 Wiley‐Liss, Inc.
Percutaneous intervention over the origin of a saphenous vein graft anastomosed with the St. Jude Medical Symmetry Aortic Connector System
Authors: Fuertes Jorge , Lozano Iñigo , Turiel Jose M. , Llosa Juan Carlos , Suarez Emma , de la Tassa Cesar Moris 
Published: December 2003

AbstractWe describe a percutaneous intervention performed over the proximal anastomoses of an aortocoronary saphenous vein graft that had been attached to the aorta 3 months before with the St. Jude Medical Symmetry Aortic Connector System. Technical aspects of procedure are discussed, and the experience previously reported in the literature related to this issue is reviewed. Catheter Cardiovasc Interv 2004;61:103–106. © 2004 Wiley‐Liss, Inc.
Subclavian artery stenting: Factors influencing long‐term outcome
Authors: Bates Mark C. , Broce Mike , Lavigne P. Scott , Stone Patrick 
Published: December 2003

AbstractThis study provides extended follow‐up of a nonrandomized series of symptomatic patients who underwent subclavian stent‐supported angioplasty (SSA) with emphasis on preprocedure factors that may have influenced outcome. The endpoints of mortality and restenosis were analyzed using backward stepwise logistic regression with the following clinical variables: coronary artery disease, hypertension, hyperlipidemia, smoking, diabetes mellitus, chronic obstructive pulmonary disease, chronic renal insufficiency/failure, and hypothyroidism. Restenosis is reported based on prospective serial noninvasive studies and/or angiography. Mortality was evaluated by retrospective database review and inquiry to the State Department of Health and Human Services' statistical registry in patients who were lost to follow‐up. Over a 9‐year period (mean follow‐up, 36.1 ± 30.4 months; maximum observation, 109.5 months), 101 stents were placed in 91 consecutive patients (37 male, 54 female). The mean age at intervention was 62.03 ± 9.3. The procedure was technically successful in 89 patients 97% (mean pre‐ and postoperative stenosis and pressure gradients were 90.2% ± 9.4% vs. 3.7% ± 6.6%, P < 0.001, and 59.9 ± 35.2 vs. 0 mm Hg, P < 0.001, respectively), with 13 minor complications and no immediate major complications. One patient died of unrelated causes within 30 days. Per Kaplan‐Meier method, for years 1 through 5, the rates of overall patency were 96%, 91%, 86%, 77%, and 72%; likewise, overall patient survival was 93%, 88%, 8%4, 81%, and 76%. No clear predictors for restenosis were discovered, although a trend toward higher recurrence was noted in women (18.5% in female vs. and 8.6% in male; P > 0.05), but the same were less likely to die during follow‐up (P > 0.001). Also, the presence of hypothyroidism (P = 0.004) and increasing age (P = 0.068) were positively correlated with all‐cause mortality. This study suggests that SSA is predictable, safe, and durable. The diagnosis of symptomatic subclavian disease is of prognostic importance, with age and male gender representing important predictors of all‐cause long‐term mortality. The strong association of increased mortality with hypothyroidism is difficult to discard and raises the question of a yet to be described thyroid steal phenomena. Catheter Cardiovasc Interv 2004;61:5–11. © 2004 Wiley‐Liss, Inc.
Preoperative balloon dilatation of obstructed total anomalous pulmonary venous connection in a neonate
Authors: Ramakrishnan Sivasubramanian , Kothari Shyam Sunder 
Published: December 2003

AbstractPrompt recognition and early surgical correction are mandatory in neonates presenting with obstructed total anomalous pulmonary venous connection (TAPVC). Preoperative balloon angioplasty of obstructed TAPVC in a sick neonate is rarely reported. We describe successful short‐term palliation with balloon angioplasty of a critically ill neonate with obstructed supracardiac TAPVC. Catheter Cardiovasc Interv 2004;61:128–130. © 2004 Wiley‐Liss, Inc.
Fallacy of thoracic side‐branch steal from the internal mammary artery: Analysis of left internal mammary artery coronary flow during thoracic side‐branch occlusion with pharmacologic and exercise‐induced hyperemia
Authors: Guzon Osler Jay J. , Klatte Karen , Moyer Andrea , Khoukaz Souheil , Kern Morton J. 
Published: December 2003

AbstractIn some patients, myocardial ischemia after coronary artery bypass graft surgery has been attributed to a coronary steal phenomenon through a thoracic side branch originating from the left internal mammary artery (LIMA), even in the absence of subclavian or LIMA stenosis. To demonstrate that coronary flow through the LIMA is unchanged by occlusion of a LIMA side branch, we examined LIMA coronary flow velocity measurements (0.014″ Doppler flow wire) in three patients at rest, during adenosine hyperemia, and again during hyperemia induced by left arm exercise before and again after the balloon occlusion of the thoracic side branch. For the three patients, no significant changes in resting or hyperemic flow were noted due to side‐branch occlusion. Before side‐branch occlusion, pharmacologic intra‐arterial (adenosine) coronary flow reserve (hyperemic‐to‐basal flow velocity ratio) was 2.6, 1.5, and 3.2 and exercise flow reserve was 2.1, 1.3, and 1.2, respectively. After side‐branch occlusion, pharmacologic coronary flow reserve was 2.5, 1.8, and 2.7 with exercise flow reserve of 1.8, 1.1, and 1.3, respectively. Under most ordinary circumstances, thoracic side‐branch steal does not exist and that side‐branch occlusion does not alter LIMA flow at rest or during pharmacologic or exercise‐induced hyperemia. These data further suggest that a demonstration of the physiologic value of side‐branch occlusion should precede surgical or percutaneous interruption of the thoracic artery in such patients. Catheter Cardiovasc Interv 2004;61:20–28. © 2004 Wiley‐Liss, Inc.
Early experience with a novel plaque excision system for the treatment of complex coronary lesions
Authors: Ikeno Fumiaki , Hinohara Tomoaki , Robertson Gregory C. , Rezaee Mehrdad , Yock Paul G. , Reimers Bernhard , Colombo Antonio , Grube Eberhard , Simpson John B. 
Published: December 2003

AbstractThe use of directional coronary atherectomy (DCA) in current practice has been limited. The SilverHawk System is a newly developed plaque excision device that aims to overcome the drawbacks of prior DCA platforms. The device was evaluated in a porcine coronary model and in a series of patients. Procedural variables along with outcomes were reviewed. Quantitative angiography (QCA) was performed and excised tissue fragments were weighed and examined histologically. In porcine cases, pretreatment MLD increased from 0.51 ± 0.26 to 2.36 ± 0.59 mm postdebulking and 19.9 ± 7.6 mg of tissue was retrieved. In human cases, pretreatment MLD increased from 0.8 ± 0.4 to 2.2 ± 0.5 mm postdebulking and 15.2 ± 7.8 mg of tissue was retrieved without complications. These data show that the SilverHawk System may offer significant utility in treating a wide variety of complex coronary lesions. Catheter Cardiovasc Interv 2004;61:35–43. © 2004 Wiley‐Liss, Inc.
Transcatheter closure of a very large patent ductus arteriosus in a pregnant woman at 22 weeks of gestation
Authors: Kanter Joshua P. , Hellenbrand William E. , Pass Robert H. 
Published: December 2003

AbstractSuccessful transcatheter closure of a large PDA in a pregnant patient is reported using the Amplatzer ductal occluder device. This procedure was safe and uncomplicated, obviating the need for surgery in this high risk patient. Catheter Cardiovasc Interv 2004;61:140–143. © 2004 Wiley‐Liss, Inc.
A young female patient with anorexia nervosa complicated by Mycobacterium szulgai pulmonary infection
Authors: Hotta Mari , Minami Yuichiro , Itoda Ichiro , Yoshimori Kozo , Takano Kazue 
Published: December 2003

AbstractObjectivePulmonary infection with a rare atypical mycobacterium, Mycobacterium szulgai, was discovered during the treatment of anorexia nervosa in a 21‐year‐old Japanese woman without preexisting pulmonary disease. She had a long history of low body weight below 35kg.MethodsOn admission, she was examined. She weighed 23kg and presented with hypoproteinemia, decreased levels of rapid turnover proteins, liver dysfunction, and decreased serum level of insulin‐like growth factor‐I.ResultsAlthough she had had neither clinical symptom specific for mycobacterium pulmonary infection nor inflammatory data, a chest roentgenogram showed an infiltrative shadow with cavity formation in the right upper lung field. Isolated bacteria from sputum was acid‐fast bacilli and identified as M. szulgai using the DNA‐DNA hybridization method.DiscussionIn anorexia nervosa patients with a long history of severe malnutrition, special attention must be paid to the possibility of opportunistic infections, even in the absence of symptoms or inflammatory data. © 2003 by Wiley Periodicals, Inc. Int J Eat Disord 35: 115–119, 2004.
Rating of figures used for body image assessment varies depending on the method of figure presentation
Authors: Doll Michelle , Ball Geoff D.C. , Willows Noreen D. 
Published: December 2003

AbstractObjectiveTo determine the effect of method of figure presentation on figure rating scales used for body image assessment.MethodsRatings of current, ideal, and socially acceptable body sizes were elicited from 360 university students. Male and female figure drawings ranging from thin to obese were presented to subjects using one of three presentation methods. Figures were presented as an ordered array, an unordered array, or they were placed individually on cards that were fixed in order from thin to obese.ResultsFigure ratings were significantly different among methods for the selection of current and ideal figure and socially acceptable body sizes.DiscussionThe method of figure presentation had an influence on figure ratings. These findings suggest that the manner in which figures are presented to research participants for the evaluation of body image constructs may be responsible, in part, for the discordant results reported in the body image assessment literature. Future research is required to address potential reasons why the method of figure presentation results in different figure ratings. © 2003 by Wiley Periodicals, Inc. Int J Eat Disord 35: 109–114, 2004.