bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biotinylated GHK peptide incorporated collagenous matrix: A novel biomaterial for dermal wound healing in rats
Authors: Arul V. , Gopinath D. , Gomathi K. , Jayakumar R. 
Published: March 2005

AbstractMatrikines are small peptide fragments of extracellular matrix proteins that display potent tissue repair activities. Difficulties in achieving sustained delivery of bioactive concentration of matrikines in the affected area limits their therapeutic use. The present study evaluates the effects biotinylated matrikine peptide (bio‐glycyl‐histidyl‐lysine) incorporated collagen membrane for dermal wound healing processes in rats. Biotinylated peptide incorporated collagen matrix (PIC) showed better healing when compared to wounds treated with collagen matrix [CF (collagen film)] and without collagen [CR (control)]. Binding studies indicate that biotinylated GHK (Bio‐GHK) binds effectively to the collagen matrix and red blood cell (RBC) membrane when compared with t‐butyloxycarbonyl substituted GHK (Boc‐GHK). Wound contraction, increased cell proliferation, and high expression of antioxidant enzymes in PIC treated group indicate enhanced wound healing activity when compared to CF and CR groups. Interestingly Bio‐GHK incorporated collagen increases the copper concentration by ninefold at the wound site indicating the wound healing property of Bio‐GHK can also be linked with both copper localization and matrikine activities. These results demonstrate the possibility of using Bio‐GHK incorporated collagen film as a therapeutic agent in the wound healing process. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater
Quantitative evaluation of the fibrin clot extension on different implant surfaces: An in vitro study
Authors: Di Iorio Donato , Traini Tonino , Degidi Marco , Caputi Sergio , Neugebauer Joerg , Piattelli Adriano 
Published: March 2005

AbstractThe aim of the present study was a quantitative evaluation of the in vitro fibrin clot extension on different implant surfaces. Forty‐five disk‐shaped commercially pure Grade 2 titanium samples with three different surface topographies (machined, DPS, and Plus) were used in the present study. For the quantitative evaluation of the fibrin clot, 30 specimens were used (10 per group); human whole blood was employed. Venous blood was drawn from three healthy adult volunteers, and 0.2 mL were immediately dropped onto the surface of each specimen. Contact time was 5 min at room temperature; then the samples were rinsed with saline solution and fixed in a buffered solution of glutaraldehyde and paraformaldehyde. Samples were washed again with buffer and dehydrated in an ascending alcohol series. Specimens belonging to all groups were observed under SEM at a magnification of 1000×. From each sample, 50 random micrographs were collected in .tif format with an N × M 1024 × 768 grid of pixels. Quantitative analysis of fibrin clot extension showed the following results: in machined samples fibrin clot extension was 345987.2 ± 63747.7 pixels2 (mean ± SD), in DPS samples fibrin clot extension was 375930.9 ± 54726.86 pixels2 (mean ± SD), and in Plus samples, fibrin clot extension was 612333.6 ± 46268.42 pixels2 (mean ± SD). With ANOVA it was possible to find that there were significant differences among the groups. The Tukey test revealed that the extension of the fibrin clot of Plus samples was statistically higher compared to both machined and DPS samples. The results of this in vitro study indicate that there is a correlation between implant surface morphology and fibrin clot extension. Improvement in surface microtexture complexity seems to determine the formation of a more extensive and three dimensionally complex fibrin scaffold. Further investigations are necessary to explain in more detail the mechanisms that regulate the fibrin clot formation on different implant surfaces. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater
Low temperature complete combustion of dilute toluene and methyl ethyl ketone over Mn‐doped ZrO2 (cubic) catalysts
Authors: Choudhary VR , Deshmukh GM , Pataskar SG 
Published: March 2005

AbstractCombustion of dilute toluene and methyl ethyl ketone over Mn‐doped ZrO2 catalysts prepared using different precipitating agents, such as tetra‐alkyl ammonium hydroxides and NH4OH, having Mn/Zr ratios from 0.05 to 0.67, and calcined at different temperatures has been thoroughly investigated. The Mn‐doped ZrO2 catalyst shows high toluene or methyl ethyl ketone combustion activity, particularly when its ZrO2 is in cubic form, when its Mn/Zr ratio is close to 0.2, and when it is prepared using tetra‐methyl ammonium hydroxide as a precipitating agent and calcined at 773 K. Copyright © 2005 Society of Chemical Industry
Cyto‐epitheliochorial placenta of the viviparous lizard Pseudemoia entrecasteauxii: A new placental morphotype
Authors: Adams Susan M. , Biazik Joanna M. , Thompson Michael B. , Murphy Christopher R. 
Published: March 2005

AbstractThe structural features of the uterine epithelium of the chorioallantoic placenta and omphalloplacenta in the viviparous Australian skink, Pseudemoia entrecasteauxii, were investigated using SEM and TEM techniques. In particular, the structural characteristics that would allow interpretation of function were analyzed, particularly those of gas exchange in the chorioallantoic placenta and histotrophy in the omphaloplacenta. Pseudemoia entrecasteauxii has a complex placenta consisting of a placentome, paraplacentome, and omphaloplacenta. The paraplacentome has a well‐vascularized lamina propria in which projecting uterine capillaries displace the overlying uterine epithelial cells, reducing them to attenuated cytoplasmic extensions. Associated cell nuclei and organelles are lost from this region, to provide a capillary lumen to uterine lumen barrier of 0.5–1.0 μm. Hence, the paraplacentome is likely a prominent site for gaseous exchange via simple diffusion. The omphaloplacenta has a similar cytology to that of the placentome, but the uterine epithelial cells are hypertrophied and the apical plasma membrane actively secretes vesicles into the uterine lumen. The omphaloplacenta shows features that are associated with histotrophic transport of nutrients via vesicle secretion, very similar to that of lipid apocrine secretion. The placentome consists of cuboidal cells in the uterine epithelium, with large centrally located nuclei overlying the well‐vascularized lamina propria. Although the placentome has a similar cytological structure to that of the omphaloplacenta, granules or active vesicle secretion were not observed. Thus, the placentome may be associated with histotrophy, but not via apocrine secretion. Squamate placentation is epitheliochorial; however, we propose a new term be used to describe the type of placentation in P. entrecasteauxii: “cyto‐epitheliochorial,” because of the extreme attenuation of uterine epithelial cells of the paraplacentome. J. Morphol. © 2005 Wiley‐Liss, Inc.
Functional morphology of the mantle of Nautilus pompilius (Mollusca, Cephalopoda)
Authors: Westermann Bettina , Schmidtberg Henrike , Beuerlein Knut 
Published: March 2005

AbstractThis study presents histological and cytological findings on the structural differentiation of the mantle of Nautilus pompilius in order to characterize the cells that are responsible for shell formation. The lateral and front mantle edges split distally into three folds: an outer, middle, and inner fold. Within the upper part of the mantle the mantle edge is divided into two folds only; the inner fold disappears where the hood is attached to the mantle. At the base of the outer fold of the lateral and front mantle edge an endo‐epithelial gland, the mantle edge gland, is localized. The gland cells are distinguished by a distinct rough endoplasmic reticulum and by numerous secretory vesicles. Furthermore, they show a strong accumulation of calcium compounds, indicating that the formation of the shell takes place in this region of the mantle. Numerous synaptic contacts between the gland cells and the axons of the nerve fibers reveal that the secretion in the area of the mantle edge gland is under nervous control. The whole mantle tissue is covered with a columnar epithelium having a microvillar border. The analyses of the outer epithelium show ultrastructural characteristics of a transport active epithelium, indicating that this region of the mantle is involved in the sclerotization of the shell. Ultrastructural findings concerning the epithelium between the outer and middle fold suggest that the periostracum is formed in this area of the mantle, as it is in other conchiferan molluscs. J. Morphol. © 2005 Wiley‐Liss, Inc.
Phenolic compounds in some apple (Malus domestica Borkh) cultivars of organic and integrated production
Authors: Veberic Robert , Trobec Mateja , Herbinger Karin , Hofer Melanie , Grill Dieter , Stampar Franci 
Published: March 2005

AbstractEleven organically grown apple cultivars and 11 apple cultivars of integrated production from Austria and Slovenia were analyzed by HPLC for the content of phenolic compounds in peel and pulp. We identified chlorogenic acid, p‐coumaric acid, procyanidin B3, protocatechuic acid, (−)‐epicatechin, phloridzin, rutin and quercetin‐3‐rhamnoside in apple peel. In apple pulp, (+)‐catechin was also identified in all the cultivars. Some other phenols (procyanidin B3, rutin and quercetin‐3‐rhamnoside) could not be identified or were not properly separated. With regard to the phenolic content in the apple peel, there were no differences between organically grown apple cultivars and apple cultivars of integrated production. Organically grown apples, however, exhibited a higher content of phenolic substances in the apple pulp compared with the apple cultivars of integrated production. This may be due either to the different genotype source or to the growing technology. Higher concentrations of phenolic compounds in organically grown cultivars could be a result of plant response to stress. The apple peel contained higher concentrations of identified phenols than the pulp. The apple peel represents up to 10% of the whole fruit; therefore the phenolsic compounds in the pulp are of greater importance to the consumer than the phenolic compounds in the peel. Copyright © 2005 Society of Chemical Industry
Determination of cooking times of pulses using an automated Mattson cooker apparatus
Authors: Wang Ning , Daun James K 
Published: March 2005

AbstractA Mattson bean cooker apparatus was modified to electronically register individual cooked seeds by automatically recording the time taken for each plunger to drop. Contact switches, interfaced with a computer via a digital input/output board, were activated when individual plungers dropped through the cooked seeds. It was found that at the time when 80% of the seeds had been penetrated the Mattson cooker method agreed with a tactile method for determining cooking times for yellow peas (Pisum sativum), lentils (Lens culinaris), chickpeas (Cicer arietinum) and navy beans (Phaseolus vulgaris). Compared with the tactile method, the automated Mattson cooker method was more objective, much easier to carry out and more resource‐efficient. The automated Mattson cooker could provide plant breeders and the pulse industry with a reference testing method for evaluating the cookability of pulses. Copyright © 2005 Society of Chemical Industry
Estimation of the degree of polymerization of condensed tannins of some wild fruits of Zimbabwe (Uapaca kirkiana and Ziziphus mauritiana) using the modified vanillin‐HCl method
Authors: Muchuweti Maud , Ndhlala Ashwell R , Kasiyamhuru Abisha 
Published: March 2005

AbstractMethanol and glacial acetic acid were compared for their effect in the vanillin assay for tannins. The time taken for the development of colour was followed using a spectrophotometer. By reacting vanillin and condensed tannins in the presence of acetic acid, the time for the development of the chromogenic substance was reduced approximately threefold compared with the time taken in the presence of methanol. The degree of polymerization was followed by reacting a catechin standard, the tannin of the Uapaca kirkiana and Ziziphus mauritiana, with vanillin in acetic acid medium at 30 °C and measuring absorbance at intervals. The values obtained suggested between 4 and 10 monomer units of catechin per polymer of tannins for the different portions of the two fruits. Copyright © 2005 Society of Chemical Industry
Quercetin properties as a functional ingredient in omega‐3 enriched fish gels fed to rats
Authors: Pérez‐Mateos Miriam , Bravo Laura , Goya Luis , Gómez‐Guillén Carmen , Montero Pilar 
Published: March 2005

AbstractWistar rats were fed with surimi gels containing either sunflower oil, fish oil (ω3), and the same formulation additionally supplemented with 1.05 g kg−1 quercetin (ω3‐Q). Antioxidant capacity was highest in gels with added quercetin when measured by the ferric‐reducing/antioxidant power (FRAP) method, but not by the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical assay. Lipid stability was not enhanced by quercetin since commercial fish oil already contains stabilizers. Quercetin modified neither rheological properties nor water‐holding capacity of the gels; however, it produced a large increase in yellowness (b*). Serum lipid profile of rats was not significantly different. Total serum antioxidant capacity by FRAP was significantly increased only in the ω3‐Q group. Plasma malondialdehyde was similar in the ω3 and ω3‐Q groups, indicating no prooxidative effect of quercetin in vivo. These results suggest that quercetin might be used as a food ingredient in fish gel to improve some nutritional properties of the gel. Copyright © 2005 Society of Chemical Industry
Pectin and calcium chloride treatment for low‐fat fried green gram splits
Authors: Holikar Manoj V , Annapure Uday S , Singhal Rekha S , Kulkarni Pushpa R 
Published: March 2005

AbstractFried green gram (Phaseolus mungo L) splits are a traditional savoury relished by consumers of all age groups as an anytime snack. The high oil content in such products is of concern owing to its association with obesity and related health disorders. The present work evaluates the effect of soaking green gram splits in calcium chloride solution (2.5–10 g kg−1) for 3 h followed by air drying and a subsequent dip in 10–30 g l−1 pectin solution for 10 min, again followed by air drying, on the oil content in the fried product prepared from them. After frying in groundnut oil at 170 ± 5 °C for 2 min, the oil content in the pretreated fried product was reduced to 211.7 g kg−1, compared with 335 g kg−1 for the untreated control. Attempts to further reduce the oil content by applying a second coating of pectin were unsuccessful. Copyright © 2005 Society of Chemical Industry
Effect of pea and soya bean interaction on zinc dialyzability in weaning foods
Authors: Rincón F , Ibáñez MV , Martínez B 
Published: March 2005

AbstractHigher values are reported for Zn dialyzability of homogenized infant formulas when pea content is high and soya bean content is low, but also when the reverse is the case, ie low pea content and high soya bean content. This apparent paradox is examined in terms of a potential antagonistic effect of the pea × soya bean interaction on Zn dialyzability. The antagonistic effect is traced to the linear combined effect of the two ingredients on pH over a narrow interval around 5.90 and on calcium content. This combined effect may account for the difference in results between in vivo and in vitro studies reported by several authors. The combination of certain vegetables in an infant formula may, in certain circumstances, enhance Zn dialyzability, contradicting the general view that increased legume content diminishes Zn dialyzability due to an increase in phytic acid levels. Copyright © 2005 Society of Chemical Industry
Positional analysis of egg triacylglycerols and phospholipids from hens fed diets enriched with refined seal blubber oil
Authors: Pacetti Deborah , Hulan Howard W , Schreiner Matthias , Boselli Emanuele , Frega Natale G 
Published: March 2005

AbstractThe effect of feeding laying hens with refined seal blubber oil (SBO, containing 22.2% ω3 polyunsaturated fatty acids) on the fatty acids composition and regiospecific distribution of fatty acids in triacylglycerols (TAG) and phospholipids (PL) of egg produced was investigated. The hens were fed four diets containing 0 (control), 1.25, 2.5 or 5% SBO for 5 and 9 weeks. Comparison of the total fatty acids composition indicated that a 5 week feeding period was sufficient to obtain the highest amount of total polyunsaturated fatty acids in the eggs. The fatty acids composition of the egg lipids reflected the fatty acids composition of the diet. The substantial reduction in the ω6/ω3 ratio (ie 8.5 to 2.6) is of interest, and is in line with the recommendations of health authorities in several countries, where it has been suggested that the human diet should contain a ω6/ω3 ratio of 3–4. The greatest reduction of the ω6/ω3 ratio occurred for diet A (control) and diet B (1.25% SBO). Principal component analysis analysis of the fatty acids composition of egg lipid showed four clusters representing: (i) the control diet; (ii) the diet containing 1.25% SBO as well as samples obtained from feeding 2.5% SBO for 9 weeks; (iii) the diet with 5% SBO; and (iv) samples obtained from hens fed 2.5% SBO for 5 weeks. More ω‐3 polyunsaturated fatty acids (ω3 PUFA) were incorporated in phosphatidylethanolamine and phosphatidylcholine than in TAG.Although ω3 were predominantly linked in the position Sn‐1,3 of TAG in SBO, they were esterified in the Sn‐2 position of the TAG and PL of eggs obtained after feeding laying hens with enriched diets. Copyright © 2005 Society of Chemical Industry
Variations in volatile compounds and flavour in Idiazabal cheese manufactured from ewe's milk in farmhouse and factory
Authors: Barron Luis , Redondo Yolanda , Aramburu Mikel , Pérez‐Elortondo Francisco J , Albisu Marta , Nájera Ana I , Renobales Mertxe de 
Published: March 2005

AbstractStage of lactation, use of bulk milk or milk from individual flocks, and cheese‐making in farmhouse or industrial factory are important factors affecting the production and quality of Idiazabal cheese. The volatile composition of cheese samples made from raw ewe's milk in farmhouses or industrial plants at two different times of the year and aged for 90 and 180 days was analysed by dynamic headspace coupled to GC‐MS. Short‐chain fatty acids, primary and secondary alcohols, methyl ketones and ethyl esters were the most abundant compounds in the aroma of Idiazabal cheese samples. Differences in the volatile composition were found between farmhouse and industrial cheeses made at different times of the year and ripened for 90 or 180 days. Likewise, the sensory profiles of the farmhouse and industrial cheeses were significantly different, regardless of the time of the year and ripening time. The results for the principal component analysis (PCA) performed on the sensory attributes of the cheese samples showed two PCs defined as ‘farmhouse flavour factor’ and ‘industrial flavour factor’. Farmhouse cheeses showed high scores for buttery, milky and toasty odours, and buttery and nutty flavours, whereas industrial cheeses showed high scores for sharp, rennet and brine odours, and rennet and rancid flavours. The percentages of methyl ketones such as 3‐hydroxy‐2‐butanone, 2‐butanone, 2‐pentanone and 2‐heptanone, and acids such as n‐propanoic, 2‐methylpropanoic and 3‐methylbutanoic acids were higher in farmhouse cheeses than in industrial cheeses. On the other hand, the percentages of esters such as ethyl butanoate and ethyl hexanoate, and alcohols such as 3‐methyl‐1‐butanol, and acids like n‐hexanoic acid were higher in industrial cheeses than in farmhouse cheeses. Relationships between sensory attributes and volatile compounds were studied on the basis of the differences found in sensory profile and volatile composition between farmhouse and industrial cheeses. Copyright © 2005 Society of Chemical Industry
Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro
Authors: Awati Ajay , Konstantinov Sergey R , Williams Barbara A , Akkermans Antoon D L , Bosch Marlou W , Smidt Hauke , Verstegen Martin W A 
Published: March 2005

AbstractThe in vitro cumulative gas production technique can be used to assess microbial activity of a complex community, in relation to fermentation of a particular energy source. Therefore, in combination with an in vivo study to examine the effects of two different diets for weaning piglets, microbial activities of faeces were compared from animals on the two different diets. The two diets were: CHO diet [containing added fermentable carbohydrates, including sugarbeet pulp (SBP) and wheat starch (WST)], and control diet without any added fermentable carbohydrates. Neither diet contained antibiotics or extra added copper. Twenty‐four piglets were selected from 12 litters (two per litter), weaned at 4 weeks of age (neither creep feeding nor any antibiotic treatment before and during the study), and introduced to one of the two diets. After 9 days on the diet, faecal samples were collected from selected animals, and tested for their activity in terms of gas production kinetics, and end‐products such as volatile fatty acids, ammonia and dry matter disappearance of the two test substrates SBP and WST. The bacterial diversity was also analysed before and after in vitro fermentation using denaturing gradient gel electrophoresis analysis of amplified 16S rRNA genes. There were differences both in kinetics and end‐products of the substrates. More interestingly, significant differences were detected between inocula, although mainly in terms of fermentation kinetics of the two substrates. With the CHO inoculum, SBP was fermented faster than with the control, while this effect was reversed for WST. Significantly higher diversity, as measured by DGGE fingerprint analysis, was detected in the microbial community enrichment on SBP as compared with WST at the end of fermentation. The difference between the kinetics of SBP compared with WST fermentation by faecal microbiota from the CHO diet fed piglets suggests better adaptation to SBP fermentation than to WST fermentation. The WST fermentation was more unexpected, given that a significant amount of starch is known to be fermentable by the small intestinal microbiota. It was concluded that the microbial community composition and activity in the GIT may be changed in response to diet, and that this change can be detected in vitro. Copyright © 2005 Society of Chemical Industry
Isolated vitamin E deficiency with demyelinating neuropathy
Authors: Puri Vinod , Chaudhry Neera , Tatke Medha , Prakash Ved 
Published: March 2005

AbstractA 22‐year‐old man, with a past history of generalized tonic‐clonic seizures treated with phenobarbital, presented with spinocerebellar ataxia. The electrophysiological studies revealed a demyelinating motor‐sensory neuropathy. The serum vitamin E level was low. Sural nerve biopsy revealed loss of large myelinated fibers with evidence of remyelination. Vitamin E supplementation led to clinical and electrophysiological recovery of sensory conduction and evoked potentials. Motor nerve conduction, however, showed only partial recovery. Vitamin E deficiency leading to a demyelinating neuropathy, as in the present case, suggests that the full spectrum of the disease entity is not fully defined. Muscle Nerve, 2005