bluuebunny/crossref_metadata_2025_split huggingface dataset:
Data‐driven modelling for online fault pre‐warning in thermal power plant using incremental Gaussian mixture regression
Authors: Jin Shengxiang , Si Fengqi , Dong Yunshan , Ren Shaojun 
Published: November 2023

AbstractThis study introduces a data‐driven model for online fault pre‐warning in thermal power plants using incremental Gaussian mixture regression. To tackle the issue of outdated parameters in existing fault pre‐warning models, this study puts forth an incremental Gaussian mixture regression that leverages the merging of Gaussian components to reconstruct the model and enable online modelling. Due to its criticality, a forgetting factor is introduced during the merging process to efficiently manage the weight allocation between present and historical patterns, thereby guaranteeing the model's accuracy. The results of the sine function case demonstrate that the incremental Gaussian mixture regression (IGMR) model exhibits excellent pattern control performance and modelling efficiency. Furthermore, the IGMR model is employed to forecast parameter alterations in pulverizer blockages with mode switching, and experimental validation indicates that IGMR precisely anticipates parameter changes following mode switching. Compared to on‐site solutions, the pre‐warning of coal blockage has a clear advantage in advance warning.
The Pseudomonas aeruginosa lectin LecB modulates intracellular reactive oxygen species production in human neutrophils
Authors: Sanchez Klose Felix P. , Dahlstrand Rudin Agnes , Bergqvist Linda , Scheffler Julia M. , Jönsson Katarina , Islander Ulrika , Karlsson‐Bengtsson Anna , Bylund Johan , Venkatakrishnan Vignesh 
Published: November 2023

AbstractPseudomonas aeruginosa is a Gram‐negative bacterium and an opportunistic pathogen ubiquitously present throughout nature. LecB, a fucose‐, and mannose‐binding lectin, is a prominent virulence factor of P. aeruginosa, which can be expressed on the bacterial surface but also be secreted. However, the LecB interaction with human immune cells remains to be characterized. Neutrophils comprise the first line of defense against infections and their production of reactive oxygen species (ROS) and release of extracellular traps (NETs) are critical antimicrobial mechanisms. When profiling the neutrophil glycome we found several glycoconjugates on granule and plasma membranes that could potentially act as LecB receptors. In line with this, we here show that soluble LecB can activate primed neutrophils to produce high levels of intracellular ROS (icROS), an effect that was inhibited by methyl fucoside. On the other hand, soluble LecB inhibits P. aeruginosa‐induced icROS production. In support of that, during phagocytosis of wild‐type and LecB‐deficient P. aeruginosa, bacteria with LecB induced less icROS production as compared with bacteria lacking the lectin. Hence, LecB can either induce or inhibit icROS production in neutrophils depending on the circumstances, demonstrating a novel and potential role for LecB as an immunomodulator of neutrophil functional responses.
A Review of Mechanistic Models for Predicting Adverse Effects in Sediment Toxicity Testing
Authors: Burgess Robert M. , Kane Driscoll Susan , Bejarano Adriana C. , Davis Craig Warren , Hermens Joop L. M. , Redman Aaron D. , Jonker Michiel T. O. 
Published: November 2023

Abstract
Since recognizing the importance of bioavailability for understanding the toxicity of chemicals in sediments, mechanistic modeling has advanced over the last 40 years by building better tools for estimating exposure and making predictions of probable adverse effects. Our review provides an up-to-date survey of the status of mechanistic modeling in contaminated sediment toxicity assessments. Relative to exposure, advances have been most substantial for non-ionic organic contaminants (NOCs) and divalent cationic metals, with several equilibrium partitioning-based (Eq-P) models having been developed. This has included the use of Abraham equations to estimate partition coefficients for environmental media. As a result of the complexity of their partitioning behavior, progress has been less substantial for ionic/polar organic contaminants. When the EqP-based estimates of exposure and bioavailability are combined with water-only effects measurements, predictions of sediment toxicity can be successfully made for NOCs and selected metals. Both species sensitivity distributions and toxicokinetic and toxicodynamic models are increasingly being applied to better predict contaminated sediment toxicity. Furthermore, for some classes of contaminants, such as polycyclic aromatic hydrocarbons, adverse effects can be modeled as mixtures, making the models useful in real-world applications, where contaminants seldomly occur individually. Despite the impressive advances in the development and application of mechanistic models to predict sediment toxicity, several critical research needs remain to be addressed. These needs and others represent the next frontier in the continuing development and application of mechanistic models for informing environmental scientists, managers, and decisions makers of the risks associated with contaminated sediments. Environ Toxicol Chem 2024;43:1778–1794. © 2023 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Embryonic Exposure to the Benzotriazole Ultraviolet Stabilizer 2-(2H-benzotriazol-2-yl)-4-methylphenol Decreases Fertility of Adult Zebrafish (Danio rerio)
Authors: Raza Yamin , Mertens Emily , Zink Lauren , Lu Zhe , Doering Jon A. , Wiseman Steve 
Published: November 2023

Abstract
Benzotriazole ultraviolet stabilizers (BUVSs) are emerging contaminants of concern. They are added to a variety of products, including building materials, personal care products, paints, and plastics, to prevent degradation caused by ultraviolet (UV) light. Despite widespread occurrence in aquatic environments, little is known regarding the effects of BUVSs on aquatic organisms. The aim of the present study was to characterize the effects of exposure to 2-(2H-benzotriazol-2-yl)-4-methylphenol (UV-P) on the reproductive success of zebrafish (Danio rerio) following embryonic exposure. Embryos were exposed, by use of microinjection, to UV-P at &lt;1.5 (control), 2.77, and 24.25 ng/g egg, and reared until sexual maturity, when reproductive performance was assessed, following which molecular and biochemical endpoints were analyzed. Exposure to UV-P did not have a significant effect on fecundity. However, there was a significant effect on fertilization success. Using UV-P-exposed males and females, fertility was decreased by 8.75% in the low treatment group and by 15.02% in the high treatment group relative to control. In a reproduction assay with UV-P-exposed males and control females, fertility was decreased by 11.47% in the high treatment group relative to the control. Embryonic exposure to UV-P might have perturbed male sex steroid synthesis as indicated by small changes in blood plasma concentrations of 17β-estradiol and 11-ketotestosterone, and small statistically nonsignificant decreases in mRNA abundances of cyp19a1a, cyp11c1, and hsd17b3. In addition, decreased transcript abundances of genes involved in spermatogenesis, such as nanos2 and dazl, were observed. Decreases in later stages of sperm development were observed, suggesting that embryonic exposure to UV-P impaired spematogenesis, resulting in decreased sperm quantity. The present study is the first to demonstrate latent effects of BUVSs, specifically on fish reproduction. Environ Toxicol Chem 2024;43:385–397. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Toxicity of Wildland Fire Retardants to Rainbow Trout in Short Exposures
Authors: Puglis Holly J. , Iacchetta Michael 
Published: November 2023

Abstract
Long-term wildland fire retardants are one important tool used to control and suppress wildfires. During suppression activities, these retardants may enter water bodies; thus, there is a need to understand their potential effects on aquatic biota. We investigated the effect of three current-use wildland fire retardants to juvenile rainbow trout (Oncorhynchus mykiss) survival in short exposures more realistic to actual intrusion scenarios. Lethal effect concentrations decreased with time and varied among chemicals (LC95A-R &gt; 259-Fx &gt; MVP-Fx). The lowest effect concentrations observed were 2 to 10 times above the threshold used by federal agencies to assess potential impacts to aquatic organisms following a retardant intrusion. These data can be used by resource managers to balance wildfire control with potential environmental impacts of retardant use. Environ Toxicol Chem 2024;43:398–404. Published 2023. This article is a U.S. Government work and is in the public domain in the USA. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
From structural design to delivery: mRNA therapeutics for cancer immunotherapy
Authors: Zhou Feng , Huang Lujia , Li Shiqin , Yang Wenfang , Chen Fangmin , Cai Zhixiong , Liu Xiaolong , Xu Wujun , Lehto Vesa‐Pekka , Lächelt Ulrich , Huang Rongqin , Shi Yang , Lammers Twan , Tao Wei , Xu Zhi Ping , Wagner Ernst , Xu Zhiai , Yu Haijun 
Published: November 2023

AbstractmRNA therapeutics have emerged as powerful tools for cancer immunotherapy in accordance with their superiority in expressing all sequence‐known proteins in vivo. In particular, with a small dosage of delivered mRNA, antigen‐presenting cells (APCs) can synthesize mutant neo‐antigens and multi‐antigens and present epitopes to T lymphocytes to elicit antitumor effects. In addition, expressing receptors like chimeric antigen receptor (CAR), T‐cell receptor (TCR), CD134, and immune‐modulating factors including cytokines, interferons, and antibodies in specific cells can enhance immunological response against tumors. With the maturation of in vitro transcription (IVT) technology, large‐scale and pure mRNA encoding specific proteins can be synthesized quickly. However, the clinical translation of mRNA‐based anticancer strategies is restricted by delivering mRNA into target organs or cells and the inadequate endosomal escape efficiency of mRNA. Recently, there have been some advances in mRNA‐based cancer immunotherapy, which can be roughly classified as modifications of the mRNA structure and the development of delivery systems, especially the lipid nanoparticle platforms. In this review, the latest strategies for overcoming the limitations of mRNA‐based cancer immunotherapies and the recent advances in delivering mRNA into specific organs and cells are summarized. Challenges and opportunities for clinical applications of mRNA‐based cancer immunotherapy are also discussed.
Language and private debt renegotiation
Authors: Godlewski Christophe J. 
Published: November 2023

AbstractWe study how language affects private debt renegotiation. We predict that stronger future time reference (FTR) languages alter the importance of renegotiation risk by lowering the perceived value of loan renegotiation. We test this hypothesis on a sample of 6500 loans issued to European firms between 1999 and 2017. We find that the use of a stronger FTR language decreases the likelihood of renegotiation and the number of renegotiation rounds. These findings are robust to several FTR proxies, various specifications including loan, borrower, and country‐level variables, and potential mitigation effects from specific loan, country, or time effects. They suggest that linguistic structure influences the renegotiation process of private debt contracts.
Development of an In Vivo Model to Study Testicular Morphogenesis
Authors: DUFOUR JANNETTE M. , RAJOTTE RAY V. , KORBUTT GREGORY S. 
Published: November 2023

ABSTRACTWe have developed an in vivo model to examine testicular cord formation by isolated Sertoli and myoid cells when implanted under the kidney capsule of severe combined immunodeficient (SCID) mice. Neonatal porcine Sertoli (92.5% ± 3.5%) and myoid (2.2% ± 0.7%) cellular aggregates were transplanted underneath the kidney capsule of SCID mice. Grafts were removed between 0 and 60 days posttransplantation and examined histologically for the progressive development of structures resembling testicular cords. Aggregates began to reorganize by day 3, and cord structures were present at day 7 posttransplantation. These structures became larger and more defined as the time progressed after implantation. To localize Sertoli and peritubular myoid cells, grafts were immunostained for the Sertoli cell proteins, vimentin, DNA transcription factor GATA‐4, and Müllerian inhibiting substance (MIS), as well as for a myoid cell protein, smooth muscle alphaactin. In the “seminiferous” epithelial layer, the Sertoli cells were arranged with their nuclei along the basal edge adjacent to the peritubular myoid cells that were surrounding the tubules. Moreover, the expression of MIS mimicked that during porcine testicular development, suggesting the Sertoli cells were developing normally. In addition, proliferating cell nuclear antigen (PCNA) was detected in the Sertoli cells at all time points, indicating the proliferation of Sertoli cells in the grafts, which is consistent with Sertoli cell proliferation prior to puberty in the native porcine testis. These results suggest that the specific factors required for cord formation and prepubertal development are inherent in the transplanted cells. Moreover, we have developed a novel in vivo transplantation model to study seminiferous cord formation and prepubertal development.
Presence of Matrix Metalloproteinases and Tissue Inhibitor of Matrix Metalloproteinase in Human Sperm
Authors: BUCHMAN‐SHAKED ORIT , KRAIEM ZAKI , GONEN YAEL , GOLDMAN SHLOMIT 
Published: November 2023

ABSTRACTThe matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that degrade protein components of the extracellular matrix. The necessity of breakdown of physical barriers in the fertilization process suggests that MMPs, along with their tissue inhibitors (TIMPs), might be involved in this task. We have examined the presence of MMP and TIMP in normal and abnormal human sperm samples by gel zymography and Western blot analysis. Thirty‐five normal sperm samples and 35 abnormal sperm samples were examined in this study. Gel zymography showed 92‐, 72‐, 62‐, and 28‐kd molecular‐weight bands exhibiting gelatin‐degrading activity in both normal and abnormal sperm samples. The 92‐, 72‐, and 62‐kd bands with gelatinolytic activity are consistent with pro‐MMP‐9, pro‐MMP‐2, and active MMP‐2, respectively (pro‐MMP being the zymogen of MMP). Western blot analysis showed the presence of TIMP‐1 in both normal and abnormal sperm samples. A higher 28‐kd activity and a lower 92‐kd MMP activity in normal sperm samples relative to abnormal samples were detected. No marked difference in TIMP‐1, 72‐kd, and 62‐kd release was observed between normal and abnormal sperm samples. In conclusion, this is the first report of MMP activity in normal and abnormal human sperm samples and of TIMP presence in sperm samples. The data indicate a different MMP profile between normal and abnormal sperm samples, with a higher 28‐kd activity and a lower 92‐kd MMP activity in normal relative to abnormal samples.
Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome
Authors: Guo Wei , Liu Haichao , Zhang Jiaxing , Zhang Jianzhong , Wang Fei , Zhang Peng , Yang Yunbo 
Published: November 2023

AbstractBladder mucosa damage that causes harm to the interstitium is a recognized pathogenesis of interstitial cystitis/bladder pain syndrome (IC/BPS). The intravesical instillation of drugs is an important second‐line therapy, but it is often necessary to use drugs repeatedly in the clinic because of their short residence time in the bladder cavity, which alters the therapeutic effect. To overcome this drawback, this study developed a novel composite acellular matrix/hyaluronic acid (HA) thermosensitive hydrogel (HA‐Gel) using rabbit small intestinal submucosa extracellular matrix (ECM) as the thermosensitive material and HA as the drug component and examined its composition, microstructure, thermodynamic properties, temperature sensitivity, rheological properties, biocompatibility, drug release, hydrogel residue, and bacteriostatic properties. The study showed HA‐Gel was liquid at temperatures of 15–37.5°C and solid at 37.5–50°C, its swelling rate decreased with increasing temperature, and its lower critical solution temperature occurred at approximately 37.5°C. This property made the hydrogel liquid at room temperature convenient for intravesical perfusion and turned into a solid about 1 min after entering the body and rising to body temperature to increase its residence time. Subsequent experiments also proved that the gel residue time of HA‐Gel in vivo and the drug release time of HA in vivo could reach more than 5 days, which was significantly higher than that of HA alone, and it had good biocompatibility and antibacterial properties. Therefore, this hydrogel possesses the proper characteristics to possibly make it an ideal dosage form for IC/BPS intravesical instillation therapy.
Application of Quantitative MRI in Thyroid Eye Disease: Imaging Techniques and Clinical Practices
Authors: Zhang Haiyang , Lu Ting , Liu Yuting , Jiang Mengda , Wang Yishi , Song Xuefei , Fan Xianqun , Zhou Huifang 
Published: November 2023

Thyroid eye disease (TED) is a complex autoimmune disorder that impairs various orbital structures, leading to cosmetic damage and vision loss. Magnetic resonance imaging (MRI) is a fundamental diagnostic tool utilized in clinical settings of TED, for its accurate demonstration of orbital lesions and indication of disease conditions. The application of quantitative MRI has brought a new prospect to the management and research of TED, offering more detailed information on morphological and functional changes in the orbit. Therefore, many researchers concentrated on the implementation of different quantitative MRI techniques on TED for the exploration of clinical practices. Despite the abundance of studies utilizing quantitative MRI in TED, there remain considerable barriers and disputes on the best exploitation of this tool. This could possibly be attributed to the complexity of TED and the fast development of MRI techniques. It is necessary that clinical and radiological aspects of quantitative MRI in TED be better integrated into comprehensive insights. Hence, this review traces back 30 years of publications regarding quantitative MRI utilized in TED and elucidates this promising application in the facets of imaging techniques and clinical practices. We believe that a deeper understanding of the application of quantitative MRI in TED will enhance the efficacy of the multidisciplinary management of TED.Level of Evidence2Technical EfficacyStage 3
Current challenges of microalgae applications: exploiting the potential of non‐conventional microalgae species
Authors: Occhipinti Paride Salvatore , Russo Nunziatina , Foti Paola , Zingale Irene Maria , Pino Alessandra , Romeo Flora Valeria , Randazzo Cinzia L. , Caggia Cinzia 
Published: November 2023

AbstractThe intensified attention to health, the growth of an elderly population, the changing lifestyles, and the medical discoveries have increased demand for natural and nutrient‐rich foods, shaping the popularity of microalgae products. Microalgae thanks to their metabolic versatility represent a promising solution for a ‘green’ economy, exploiting non‐arable land, non‐potable water, capturing carbon dioxide (CO2) and solar energy. The interest in microalgae is justified by their high content of bioactive molecules, such as amino acids, peptides, proteins, carbohydrates, polysaccharides, polyunsaturated fatty acids (as ω‐3 fatty acids), pigments (as β‐carotene, astaxanthin, fucoxanthin, phycocyanin, zeaxanthin and lutein), or mineral elements. Such molecules are of interest for human and animal nutrition, cosmetic and biofuel production, for which microalgae are potential renewable sources. Microalgae, also, represent effective biological systems for treating a variety of wastewaters and can be used as a CO2 mitigation approach, helping to combat greenhouse gases and global warming emergencies. Recently a growing interest has focused on extremophilic microalgae species, which are easier to cultivate axenically and represent good candidates for open pond cultivation. In some cases, the cultivation and/or harvesting systems are still immature, but novel techniques appear as promising solutions to overcome such barriers. This review provides an overview on the actual microalgae cultivation systems and the current state of their biotechnological applications to obtain high value compounds or ingredients. Moreover, potential and future research opportunities for environment, human and animal benefits are pointed out. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Diversified vegetation types on rangelands promote multiple soil‐based ecosystem services
Authors: Waterhouse Hannah , Aburto Felipe , Rees Gordon , Griffin‐LaHue Deirdre E. , Salls Wilson B. , Rippner Devin A. , Tian Zhiyuan , Scow Kate , O'Geen Anthony T. 
Published: November 2023

AbstractRangelands have the potential to be provisioners of ecosystem services, including livestock products, carbon storage and greenhouse gas regulation, water and nutrient cycling, wildlife habitat, and biodiversity. Due to their vast extent and landscape heterogeneity, the degree to which different ecological components of rangelands contribute to ecosystem services can be varied. Soils are the foundation of rangeland health and associated ecosystem services. While many studies have examined the effect of grazing intensity on rangeland ecosystem services, few studies have looked at the broader rangeland landscape and how managing varying vegetation types can influence soil‐based ecosystem services. In this study, a suite of physical, chemical, and biological soil health indicators were measured in various vegetation types found within a working cattle ranch, including coastal live oak woodlands, coastal scrublands, annual grassland, and restored native perennial grassland. Based on the measured soil health indicators, results from this study show scrubland significantly diverges from other vegetation types, having higher water infiltration and plant available water, carbon stocks, and a more diverse microbial community that drives more dynamic cycling of carbon and nitrogen. Strategically maintaining scrubland on unproductive, highly erosive slopes downgradient of highly productive grassland areas could maintain forage production while protecting water quality and increasing carbon storage. These results highlight the relevance of holistically evaluating rangeland operations to assess soil function and ecosystem services and the potential risks and co‐benefits of varying vegetation types. Ultimately, process‐based linkages described here may provide a working example of how to manage ranches as functional mosaics of strategically maintained vegetation types.