bluuebunny/crossref_metadata_2025_split huggingface dataset:
The G-quadruplex experimental drug QN-302 impairs liposarcoma cell growth by inhibiting MDM2 expression and restoring p53 levels
Authors: Tosoni Beatrice , Naghshineh Eisa , Zanin Irene , Gallina Irene , Di Pietro Lorenzo , Cleris Loredana , Nadai Matteo , Lecchi Mara , Verderio Paolo , Pratesi Pietro , Pasquali Sandro , Zaffaroni Nadia , Neidle Stephen , Folini Marco , Richter Sara N 
Published: January 2025

Abstract
Well-differentiated/dedifferentiated liposarcomas (WD/DDLPSs) account for ∼60% of all liposarcomas. They have a poor prognosis due to limited therapeutic options. WD/DDLPSs are characterized by aberrant expression of mouse double minute 2 (MDM2), which forms G-quadruplexes (G4s) in its promoter. Here, we investigated the possibility of targeting WD/DDLPSs with small molecules against the MDM2 G4s. Among the molecules tested, the naphthalene diimide derivative QN-302 significantly impaired WD/DDLPS cell growth and its activity strikingly paralleled cell-specific G4 abundance as measured by CUT&Tag and RNA sequencing analysis. QN-302 stabilized MDM2 G4s at the P2 inducible promoter and prevented polymerase progression from the constitutive P1 promoter, thereby inhibiting the formation of full-length MDM2 transcripts. This resulted in the accumulation of p53 through the p53-MDM2 autoregulatory feedback loop, ultimately leading to apoptotic cell death. In patient-derived xenograft mouse models, QN-302 treatment reduced tumour volume distribution and was well tolerated. We have identified a novel and effective therapeutic strategy to reduce MDM2 expression and promote p53 reactivation in tumours harbouring wild-type TP53, such as WD/DDLPSs.
Vital rates of intermittent nonbreeders and returning breeders strongly influence population dynamics of Somateria mollissima (Common Eider)
Authors: Nicol-Harper Alex , Wood Kevin A , Hilton Geoff M , Doncaster C Patrick , Ezard Thomas H G 
Published: January 2025

Abstract
Risks and costs associated with reproduction may induce iteroparous species to employ intermittent breeding, whereby individuals forgo attempting reproduction in some breeding periods following recruitment to the breeding population. We explore this behavior through population-level analyses of Somateria mollissima (Common Eider), quantifying the survival cost of breeding and hence one benefit of skipping. Nonbreeding in a given year may be a short-term response to expected low fitness returns, avoiding either breeding-induced mortality or low offspring value. Alternatively, or additionally, intermittent breeding may be a long-term strategy maximizing lifetime fitness, with breeding trajectories that include nonbreeding years resulting in more recruited offspring over the whole life course than those with the same number of consecutive breeding attempts. Reanalysis of 3 studies reporting annual mortality schedules for S. mollissima allowed estimation of the proportion of mortality incurred during the peak breeding season (~50%) and hence the difference in survival rates between breeders and nonbreeders. These were incorporated into a life cycle and associated matrix population model with a “refreshed breeder” stage to which individuals transition for the time step following nonbreeding. We show that the transition to this stage strongly influences population growth rate—being more than twice as important as reproduction by continued breeders—mostly driven by the possibility of differential survival after skipping breeding. Our results emphasize the benefits of long-term individual-based studies that can identify refreshed breeders to further our understanding of intermittent breeding. Specifically, the accuracy of population projections could be improved by factoring in transitions following skipped breeding, and management enhanced by interventions facilitating return to the breeding pool, such as nest shelter provision.
The evolution of ‘theories of harm’ in EU merger control
Authors: Duso Tomaso , Bernhardt Lea , Piechucka Joanna 
Published: January 2025

Abstract
We discuss the main theories of harm in EU merger control and their evolution since the 1990s. We present stylized facts and trends using data extracted from EU merger decisions by natural language processing tools. EU merger policy has adapted over time, both in terms of legislation and theories of harm, as well as in terms of the investigative tools and evidence used. The introduction of the new Merger Regulation in 2004, which led to a change in the substantive test, also brought about significant changes in the use of theories of harm. Unilateral theories are now used more frequently and have developed further, in particular in relation to the assessment of closeness of competition. Non-horizontal conglomerate and vertical theories of harm focusing on foreclosure issues are now much more common and are a standard tool in most in-depth investigations. More novel theories of harm related to innovation and digital markets have been developed and implemented since the 2010s. While market shares remain a central tool for merger assessment, the use of internal documents has increased, accompanied by the use of quantitative tools. With respect to Commission interventions, structural remedies are used more frequently, although behavioural remedies are also increasingly deployed, especially in Phase II.
Artificial intelligence recommendations: evidence, issues, and policy
Authors: Calvano Emilio , Calzolari Giacomo , Denicolò Vincenzo , Pastorello Sergio 
Published: January 2025

Abstract
Recommender systems (RS) enhance user experiences by providing personalized content and are widely used by popular services like Apple Music, Spotify, Netflix, and YouTube to increase user engagement. However, these systems can also have significant economic implications, including exacerbating market concentration and reducing content diversity. This paper reviews recent economic literature on RS, emphasizing their dual role as both beneficial tools and potential sources of market distortion. The paper underscores the necessity for policies informed by economic research to balance the benefits of RS against their associated risks.
Universal health-related social needs screening in a pediatric rheumatology clinic
Authors: Chandler Mia T , Biancarelli Maria Alfieri , Dekermanji Anthony , Taggart Margaret , Ethier Benjamin G , Chang Joyce C , Esty Brittany , Son Mary Beth F , Hazen Melissa M 
Published: January 2025

Abstract:

Objectives
Health-related social needs (HRSN) have a significant impact on health outcomes. While screening is prevalent in pediatric primary care settings, little evidence exists regarding the feasibility of HRSN screening in pediatric rheumatology clinics. Individuals with pediatric rheumatic disease have frequent longitudinal visits, therefore it is important to understand social needs that may hinder optimal health outcomes in this population. The objectives of this study were to understand the burden of HRSN and to contextualize the feasibility of universal HRSN screening in a busy pediatric rheumatology clinic.

Methods
The study team secured funding for a full-time social worker and developed a needs assessment questionnaire (NAQ) through an iterative process. The paper-based NAQ was distributed by administrative clinical staff to all patients at each in-person visit. Medical providers returned completed NAQs to a designated collection tray. The responses were reviewed and analyzed. The social worker addressed positive screens among families who opted-in to contact.

Results
Over 1/3 of patients returned an NAQ (985/2819), 604/985 (61%) of which revealed ≥ 1 concern. The most frequent social concern was school or work related. The social worker responded by phone or patient electronic portal message to returned NAQs that identified unmet needs.

Conclusion
A school or work concern was a frequent health-related social need in a pediatric rheumatology clinic. The prevalence of unmet HRSN, despite primary care access, show the importance of screening in rheumatology clinics. Sustainable funding is needed to scale efforts to assess and address them.
Atypical attentional network dynamics in adolescent depression during emotional movie viewing
Authors: Cahart Marie-Stephanie , Giampietro Vincent , O’Daly Owen 
Published: January 2025

Abstract
Emotion studies have commonly reported atypical emotional processing in clinically depressed adolescents in the context of short-lasting emotional cues. However, interindividual differences in the moment-to-moment brain network dynamics that underlie this impaired emotional reactivity remain unclear, and the use of poorly matched controls and relatively small sample sizes represents major limitations in most neuroimaging depression studies to date. Here, we address these concerns by using the temporal features of a rich naturalistic paradigm (i.e. a clip from the movie ‘Despicable Me’) to investigate brain network dynamics in 42 clinically depressed and 42 nondepressed adolescents aged 16–21 years, matched for age, gender, and psychiatric comorbidities. Using a dynamics functional connectivity analysis technique called Leading Eigenvector Dynamics Analysis, we found that the clinical group exhibited significantly higher probability of occurrence of the dorsal attention network and lower recruitment of the fronto-parietal, default mode network, ventral attention, and somato-motor networks throughout the task. This brain/behaviour relationship was prominent during less emotional moments of the movie, consistent with previous findings. Our findings demonstrate the key role of continuous affective measures in providing information about how activity in the depressed brain evolves as emotional intensity unfolds throughout the movie. Future studies with a larger sample size are needed in order to corroborate the present findings.
Who gets a second chance? Compliance, classification, and criminal conviction
Authors: Bing Lindsay , Gutierrez Carmen 
Published: January 2025

Abstract
Felony conviction carries lifelong consequences that impact civic, economic, and social rights and opportunities, yet not everyone who is found guilty of a felony will bear the mark of conviction. Deferred adjudication is an increasingly popular intervention that offers legally guilty defendants protection from the mark of conviction conditional on the completion of community supervision. By conditioning conviction on discretionary assessments of compliance, rather than legal establishment of guilt, deferral and similar interventions may exacerbate inequality and further concentrate the mark of conviction among marginalized groups. However, relatively few studies examine disparities in the decision to defer conviction and dismiss charges. In this study, we draw on twenty years of court records to ask “for whom is the mark of conviction and formal punishment dependent on compliance rather than the legal establishment of guilt, and who passes the test of compliance?” Findings reveal that, even when accounting for features of the offense, both race and socioeconomic status condition who gets a “second chance” at a clean record. These findings have implications for how we study inequality in criminal courts and understand the production and meaning of conviction.
Priapism-related biomarkers in sickle cell disease: a systematic review
Authors: Adesanya Oluwafolajimi , Burnett Arthur L 
Published: January 2025

Abstract

Introduction
Priapism is a major clinical complication of sickle cell disease (SCD), with severe sexual, reproductive, and mental health impact. There are currently no consensus diagnostic biomarkers for identifying individuals with SCD at risk of priapism before its occurrence.

Objectives
To systematically review the biochemical, hematological, imaging, genetic, and rheological parameters associated with priapism occurrence among individuals with SCD.

Methods
A systematic literature search for studies investigating the association of biochemical, hematological, rheological, imaging, rheological, and genetic parameters with the occurrence of priapism in individuals with SCD was performed in the MEDLINE, Embase, and Cochrane databases, using the following terms: “priapism,” “sickle cell,” “biomarker,” “marker,” “laboratory,” “radiographic,” “diagnostic,” and “predictive.” A systematic review of the identified studies was conducted to describe the landscape of priapism-related biomarkers in individuals with SCD.

Results
A total of 358 studies were identified, of which 14 studies were eventually selected for evidence synthesis. The selected studies were published between 2005 and 2023, with authorship spanning five continents. We identified multiple clinical parameters investigated as potential biomarker candidates for their association with priapism occurrence in patients with SCD. We classified these into biochemical (lactate dehydrogenase, bilirubin, aspartate transaminase, alanine transaminase, alkaline phosphatase, nitric oxide metabolites, interleukin 6), hematological (hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, reticulocyte count, leukocyte count), genetic (Klotho, TGFBR3, QAP1, ITGAV, LNC02537, NAALADL2), rheological (red blood cell deformability, aggregation index, augmentation index), and imaging parameters. However, the results were often contradictory and do not support the clinical application of any of the investigated parameters.

Conclusion
Several clinical and laboratory parameters have been associated with priapism occurrence in SCD; however, contradictory findings across geographical locations paint an unreliable picture of their clinical utility. Additional studies are needed to generate enough level 1 evidence in support of any of the current candidates.
Choosing the right signaling pathway: hormone responses to Phytophthora cinnamomi during compatible and incompatible interactions with chestnut (Castanea spp.)
Authors: Camisón Álvaro , Monteiro Pedro , Dorado Javier , Sánchez-Bel Paloma , Leitão Frederico , Meijón Mónica , Pinto Gloria 
Published: January 2025

Abstract
Ink disease caused by the hemibiotrophic root pathogen Phytophthora cinnamomi (Pc) is devastating for the European chestnut (Castanea sativa), unlike Asian chestnuts and interspecific hybrids which are resistant to Pc. The role that hormone responses play for Pc resistance remains little understood, especially regarding the temporal regulation of hormone responses. We explored the relationship between changes in tree health and physiology and alterations in leaf and root phytohormones and primary and secondary metabolites during compatible and incompatible Castanea spp.-Pc interactions. Susceptible (S) C. sativa and resistant (R) C. sativa x C. crenata ramets were inoculated with Pc in roots and assessed for disease progression, leaf physiology and biochemistry at 1, 3, 5 and 8 days after inoculation (dai). In S chestnuts, Pc increasingly deteriorated the leaf physiological functioning by decreasing leaf CO2 assimilation, stomatal conductance, transpiration rate, chlorophylls content and the maximum quantum yield of the photosystem II over time, triggering aerial symptoms (leaf wilting and chlorosis) 8 dai. Pc had little impact on the leaf physiological functioning of R chestnuts which remained asymptomatic. In roots of S chestnuts, Pc transiently induced jasmonates signaling (5 dai) while impairing root carbohydrates metabolism over time. In leaves, a transient antioxidant burst (5 dai) followed by abscisic acid (ABA) accumulation (8 dai) was observed. R chestnuts responded to Pc by up-regulating root salicylic acid (SA) at early (1 dai) and late (8 dai) infection stages, in an antagonistic crosstalk with root ABA. Overall, the results pinpoint an important role of SA in mediating the resistant response of chestnuts to Pc, but also show that the specific hormone pathways induced by Pc are genotype dependent. The study also highlights that the dynamic nature of hormone responses over time must be considered when elucidating hormone-regulated responses to Pc.
Anatomy and revised diagnosis of the embolomere Calligenethlon watsoni from Joggins, Nova Scotia, based on micro-computed tomography
Authors: Adams Gabrielle R , Otoo Benjamin K A , Bohus Caleb P W , Micucci Logan M , Maddin Hillary C 
Published: January 2025

Abstract
Although Calligenethlon watsoni, an enigmatic embolomere from Joggins, Nova Scotia, has been known since 1934, an in-depth description of its anatomy (including CT data) and updated taxonomic diagnosis has yet to be completed. Additionally, subsequently discovered embolomere specimens have been referred to the taxon simply if they display embolomere traits. As a result, Calligenethlon is represented by a collection of specimens united on the basis of largely non-diagnostic traits. Here, the exquisitely preserved specimen identified as Calligenethlon, NSM 994GF1.1, is described in detail using micro-computed tomography. Comparison of this specimen to the holotype supports its referral to Calligenethlon and the anatomical knowledge gained here allows for the generation of a more robust morphological diagnosis of the genus Calligenethlon. We then re-evaluate all other referred specimens to determine which are consistent with their referral to the taxon, and which are inconclusively referred to the taxon. These data are discussed as they provide new insights into the anatomy of Calligenethlon and the diversity of embolomeres at the Joggins’ locality.
The Efficacy of Cryoneurolysis on Chronic Pain in Patients with Knee Osteoarthritis; A Double-blinded Randomized Controlled Sham Trial
Authors: Nygaard Niels-Peter Brøchner , Koch-Jensen Carsten , Vaegter Henrik Bjarke , Wedderkopp Niels , Blichfeldt-Eckhardt Morten , Gram Bibi 
Published: January 2025

Background:
The objective was to investigate if cryoneurolysis were superior to sham in reducing pain intensity in patients with chronic knee osteoarthritis. We hypothesized that cryoneurolysis was an effective and safe therapy to reduce chronic pain in patients with knee osteoarthritis.

Methods:
The study was a randomized, double-blind, sham-controlled. Eighty-seven patients with chronic knee osteoarthritis were randomized to either cryoneurolysis or a sham procedure, followed by an exercise program. The target nerves, anterior femoral cutaneous nerve and infrapatellar branch of the saphenous nerve were identified using transcutaneous electrical nerve stimulation and cryoneurolysis was performed ultrasound-guided. The primary outcome was the difference in average pain at 14d post intervention between the cryoneurolysis and sham group.

Results:
For the intention to treat analysis, no difference was observed for the primary outcome (0.49, 95% CI [-0.3 ; 1.2], p=0.198). Both groups showed a significant reduction in pain scores 14d post intervention (Cryoneurolysis: -1.9, 95% CI [-2.4 ; -1.3], Sham: -1.4, 95% CI [-1.9 ; -0.8]). At 6 months follow-up, a significant reduction in pain was observed in the cryoneurolysis group compared to sham group (1.1, 95% CI [0.3 ; 1.9], p=0.009). Cryoneurolysis was well-tolerated, with minor, transient adverse effects.

Conclusions:
This study did not show a clinically relevant reduction in pain after intervention comparing cryoneurolysis and sham. Further studies are needed.
Double Fluoroscopy Techniques for Closed Reduction and Percutaneous Pinning in Pediatric Supracondylar Humeral Fractures: Fluoroscope Positioning and a Case Series
Authors: Ohmori Takao , Toda Kazukiyo , Hasegawa Yasuhiro , Ito Yasuo 
Published: January 2025

Objective:
Percutaneous pinning for supracondylar humeral fractures (SHFs) requires precise pin insertion while maintaining reduction making fluoroscopy indispensable for this procedure. However, it is currently difficult to obtain good-quality two-directional images with one fluoroscopy. This study aimed to report the outcomes of a closed reduction and internal fixation technique using 2 fluoroscopes simultaneously for pediatric SHF.

Patients and Methods:
Thirty-eight children were identified as having SHF (aged <16 y) who underwent closed reduction and percutaneous pinning. Regarding fracture type (Gartland classification), 10 patients had type II fractures, and 28 patients had type III fractures. The first fluoroscope used for the anteroposterior view was positioned from the patient’s cephalad side. The second fluoroscope used for the lateral view was positioned from the patient’s caudal side. By arranging 2 fluoroscopes from the craniocaudal side, it was possible to secure sufficient standing space for operators who maintained the reduction and inserted the pins.

Results:
Although this method was intricate and time-consuming for preoperative positioning during fluoroscopy, it was possible to monitor accurate images in 2 directions simultaneously during surgery. Consequently, we were able to confirm the insertion position and direction of the Kirschner wire easily and accurately while confirming the reduction. There were no complications.

Conclusion:
Procedures using 2 fluoroscopes simultaneously make it possible to confirm the insertion position and direction of the Kirschner wire easily and accurately while confirming the reduction.
Social Determinants of Self-Medication Practice by Caregivers Among Pediatrics Population in Pakistan: A Logistic Regression Analysis of a Prospective Cross-Sectional Study
Authors: Ghani Abdul , Kumari Usha , Yaqoob Uzair , Hassan Zair , Trang Vu Thi Thu , Zahid Shanzay , Khan Aarash 
Published: January 2025

Introduction and importance:
Gastric glomus tumors (GGT) are rare soft tissue tumors of the gastrointestinal tracts (GIT). It is somewhat challenging to establish the diagnosis of GGT and differentiate it from the more common submucosal neoplasms.

Case presentation
: A 34-year-old female patient presented with upper gastrointestinal bleeding. Extensive workup including endoscopic ultrasonography (EUS) revealed a well-circumscribed isoechoic mass arising from the muscularis propria. Based on fine needle biopsy (FNB) findings, with H&E stains performed only initially, the mass was considered a neuroendocrine tumor (NET). Antrectomy with Billroth II anastomosis was performed. A microscopic and immunohistochemical studies of the resected specimen showed the cells to be positive for smooth muscle actin (SMA) making GGT the final diagnosis.

Clinical Discussion:
Of the 116 patients included in our analysis, 56.9% (n=66) were females and age group was between 41-64 years old in 63.8% (n=74) of the patients. About 55 cases (47.4%) had abdominal or epigastric pain or discomfort, which was the most frequent clinical symptom. In immunohistochemistry, SMA staining is present in 68.1% of the cases, underscoring its diagnostic significance. Laparotomy with wedge or partial gastrectomy was employed in 46.1% of the recorded cases. Due to malignant potential, long-term follow-up and monitoring are usually recommended.

Conclusion:
Despite the rarity of GGT, they should be included in the differential diagnosis of gastric submucosal tumors, with immunohistochemistry studies playing a major role in the diagnosis. Furthermore, a comprehensive evaluation of the literature in the past eight years was presented in a table.