bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cabozantinib-induced heart failure
Authors: Sekmek Serhat , Bayram Dogan , Ucar Gokhan , Civelek Burak , Kos Fahriye Tugba , Uncu Dogan 
Published: October 2023

Introduction Cabozantinib is a multikinase inhibitor agent used in the treatment of hepatocellular, renal, and thyroid cancers. The development of heart failure after cabozantinib initiation is an extremely rare side effect, with only four case reports published in the literature. We describe a case of cabozantinib-induced cardiac failure in a patient with thyroid cancer refractory to standard treatment. Case report Fifty-seven-year-old woman had no history of cardiovascular disease. Echocardiography prior to chemotherapy revealed normal cardiac function. However, she developed pretibial edema and shortness of breath after 2 months of cabozantinib treatment. Ejection fraction was found to be 30% in the echocardiography of the patient, and global hypokinesia was detected in cardiac functions. Management and outcome Cabozantinib treatment of the patient was discontinued. After discontinuation of treatment, the patient's cardiac functions did not return to normal. Heart failure due to cabozantinib treatment was thought to be permanent. Discussion There are only four cases on this subject in the literature. Although the use of cabozantinib rarely causes heart failure, this side effect can have extremely serious consequences. Even if it is a rare condition, cardiological evaluations should be performed before and after cabozantinib therapy because it can be reversible after discontinuation of the treatment.
Ocular syphilis masquerading as CMV retinitis in a transplanted patient
Authors: Rissotto Federico , Scandale Pierluigi , Miserocchi Elisabetta 
Published: October 2023

Background Syphilis has historically been referred to as “the great imitator”, for the extent of disease manifestations secondary to infection. Ocular manifestations include a wide range of intra-ocular inflammation. Methods In this study, we report the case of a 52 years-old male patient with syphilitic hemorrhagic necrotizing retinitis. Results The patient presented to the emergency room for rapid and progressive vision loss and ocular redness lasting three weeks and was under immunosuppressive treatment. The diagnosis was syphilitic hemorrhagic necrotizing retinitis mimicking the typical clinical picture of retinitis caused by Cytomegalovirus infection in immunocompromised patients. Conclusions The presented case highlights the need to consider ocular syphilis as a great masquerader even in the presence of atypical presentations such as hemorrhagic retinitis. Syphilis should be tested for treponemal and non-treponemal tests, and it should be ruled out as an etiological agent in every case of new-onset intra-ocular inflammation.
Analysis of treatment cost differences in patients undergoing femoral versus radial access in outpatient diagnostic cerebral arteriograms
Authors: Findlay Matthew C , Baker Cordell M , Childs Sarah , Gautam Diwas , Salah Walid K , Bounajem Michael , Joyce Evan , Budohoski Karol P , Kilburg Craig , Alexander Matthew D , Taussky Phillip , Grandhi Ramesh 
Published: October 2023

Background Outpatient diagnostic cerebral arteriograms are the most common procedure in neuroendovascular surgery, and the use of transradial access for these studies is growing. Although transradial access has been associated with lower hospital costs for elective diagnostic and interventional neuroendovascular procedures, no study has compared transfemoral access and transradial access costs for a homogenous population of patients undergoing outpatient diagnostic cerebral arteriogram. Methods In this single-center retrospective study, the Value Driven Outcomes database was used to evaluate treatment costs for patients who underwent outpatient diagnostic cerebral arteriogram from January 2019 to December 2022. Propensity-score matching was performed to reduce confounders. Costs from each encounter were subcategorized into imaging, supplies, pharmacy, procedures, labs, and facility costs. Results After matching, 337 patients each for transradial access and transfemoral access were available for analysis. A total of 118,992 cost data points were associated with all encounters. Overall, per-visit costs were 15.2% cheaper for patients who underwent transradial access versus transfemoral access ( p < 0.001). Most of the cost difference was due to supplies (35.2% cost difference, p < 0.001) and procedure costs (9.3% cost difference, p < 0.001). No statistical differences were observed between the two approaches in imaging, pharmacy, labs, and facility costs (all p > 0.05). Conclusions Costs for outpatient diagnostic cerebral arteriogram were lower in patients who underwent transradial access versus transfemoral access because of supply and procedure costs. Understanding reasons for cost differences in common procedures is important for creating strategies to reduce overall healthcare costs. Additionally, addressing the cost differences of newer techniques may increase the likelihood that they are more readily implemented by hospitals and providers.
Comparison of the profiles of first-line PD-1/PD-L1 inhibitors for advanced NSCLC lacking driver gene mutations: a systematic review and Bayesian network meta-analysis
Authors: Wenfan Fu , Manman Xu , Xingyuan Shi , Zeyong Jiang , Jian Zhao , Lu Dai 
Published: October 2023

Background: Numerous first-line immune checkpoint inhibitors (ICI) were developed for patients with advanced non-small cell lung cancer (NSCLC) lacking driver gene mutations. However, this group consists of a heterogeneous patient population, for whom the optimal therapeutic choice is yet to be confirmed. Objective: To identify the best first-line immunotherapy regimen for overall advanced NSCLC patients and different subgroups. Design: Systematic review and Bayesian network meta-analysis (NMA). Methods: We searched several databases to retrieve relevant literature. We performed Bayesian NMA for the overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and treatment-related adverse events (tr-AEs) with a grade equal or more than 3 (grade ⩾ 3 tr-AEs). Subgroup analysis was conducted according to programed death ligand 1 (PD-L1) levels, histologic type, central nervous system (CNS) metastases and tobacco use history. Results: For the PD-L1 non-selective patients, sintilimab plus chemotherapy (sinti-chemo) provided the best OS [hazard ratio (HR) = 0.59, 95% confidence interval (CI):0.42–0.83]. Nivolumab plus bevacizumab plus chemotherapy (nivo-bev-chemo) was comparable to atezolizumab plus bevacizumab plus chemotherapy (atezo-bev-chemo) in prolonging PFS (HR = 0.99, 95% CI: 0.51–1.91). Atezo-bev-chemo remarkably elevated the ORR than chemotherapy (OR = 3.13, 95% CI: 1.51–6.59). Subgroup analysis showed pembrolizumab plus chemotherapy (pembro-chemo) ranked first in OS in subgroups of PD-L1 < 1%, non-squamous, no CNS metastases, with or without smoking history, and ranked second in OS in subgroups of PD-L1 ⩾ 1% and PD-L1 1–49%. Cemiplimab and sugemalimab plus chemotherapy ranked first in OS and PFS for squamous subgroup, respectively. For patients with CNS metastases, nivolumab plus ipilimumab plus chemotherapy (nivo-ipili-chemo) and camrelizumab plus chemotherapy provided the best OS and PFS, respectively. Conclusions: Sinti-chemo and nivo-bev-chemo were two effective first-line regimens ranked first in OS and PFS for overall patients, respectively. Pembro-chemo was favorable for patients in subgroups of PD-L1 < 1%, PD-L1 ⩾ 1%, PD-L1 1–49%, non-squamous, no CNS metastases, with or without smoking history. Addition of bevacizumab consistently provided with favorable PFS results in patients of all PD-L1 levels. Cemiplimab was the best option in squamous subgroup and nivo-ipili-chemo in CNS metastases subgroup due to their advantages in OS.
Does participation in municipality-initiated incentivized health promotion programs promote physical activity among the physically inactive? Verification in six Japanese municipalities
Authors: Chijiki Shoko , Tanabe Kai , Tsukao Akiko , Kuno Shinya 
Published: October 2023

Background: Physical activity can prevent lifestyle-related diseases, such as hypertension and diabetes. However, many people in society are physically inactive, especially middle-aged and older adults over 40. Therefore, this study examined the effects of a municipality-led incentivized health promotion program to clarify: (1) whether the average number of steps increased from the preparticipation period to the 18th month following the program; and (2) whether such increases were influenced by financial incentives. Design and methods: This study analyzed gender, age, step, and incentive type data from 5688 middle-aged and elderly participants in an incentivized health promotion program. The incentives were regional gift certificates that could only be used in local areas, national gift certificates, common points that could be used in department and convenience stores nationwide, and donations. The incentives were worth a maximum of 24,000 yen per year. Results: Both the physically active group and the physically inactive group had increased the step count markedly 18 months post-participation; however those participants chosing financial incentives showed significantly higher steps after 18 months than those who opted for non-financial incentives (time: p < 0.05, time × group: p < 0.05). Conclusions: Municipality-led financially incentivized health promotion programs could motivate increases in the average number of steps taken by those physically inactive residents.
Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction Using the Anatomic Double-Bundle 2-in-1 Guide System
Authors: Nakanishi Yuta , Hoshino Yuichi , Nukuto Koji , Nishida Kyohei , Nagai Kanto , Kanzaki Noriyuki , Matsushita Takehiko , Kuroda Ryosuke 
Published: October 2023

Background: In double-bundle anterior cruciate ligament (ACL) reconstruction, tunnel coalition may occur intraoperatively or during the postoperative course. Tibial tunnel coalition is more common compared with femoral tunnel coalition. Once tunnel coalition occurs on the tibial side, rotatory knee laxity may not be controlled as expected. We have developed a new device to avoid tibial tunnel coalition with consistency. The purpose of this video is to present the surgical technique for double-bundle ACL reconstruction using a new drill guide. Indications: The novel guide may be used in all cases with confirmed ACL tear in a physically active patient, identical to indications for current ACL reconstruction using the double-bundle technique. Technique Description: The hamstring tendon is harvested for the ACL grafts. Two guide pins for the anteromedial bundle and posterolateral bundle for the tibial tunnel are inserted through the Anatomic Double-Bundle 2-in-1 Guide System. Cannulated drills and dilators are used to create the tunnel to the final diameter. Next, femoral tunnels are created by the outside-in technique using the Anatomic Double-Bundle 2-in-1 Guide System. Grafts are inserted from the tibia and passed through the femur. The grafts are fixed with a post screw and/or interference screw. Results: Two weeks after surgery, no tibial or femoral coalition (0/20 cases) were confirmed and tibial bony bridge at the intraarticular surface was measured 2.7 ± 0.9 mm using computed tomography (CT). One year after surgery, tibial coalition was confirmed in 13.3% (2/15 cases), and femoral coalition in 6.7% (1/15 cases) on CT image mainly due to tunnel widening. The 2 cases with tibial coalition had tibial bony bridge of less than 2 mm on immediate postoperative CT. Discussion/Conclusion: Using the novel guide, 2 tibial tunnels were created easily and accurately compared with the conventional independent drilling technique. The 2 tunnels can also be created simultaneously with single placement of the guide. Two separate tunnels help maintain expected rotatory knee stability after double-bundle ACL reconstruction. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
GFAP-NpHR mediated optogenetic inhibition of trigeminal nucleus caudalis attenuates hypersensitive behaviors and thalamic discharge attributed to infraorbital nerve constriction injury
Authors: KC Elina , Islam Jaisan , Kim Hyong Kyu , Park Young Seok 
Published: October 2023

AbstractThe significance of hyperactive astrocytes in neuropathic pain is crucial. However, the association between medullary astrocytes and trigeminal neuralgia (TN)-related pain processing is unclear. Here, we examined how optogenetic inhibition of medullary astrocytes in the trigeminal nucleus caudalis (TNC) regulates pain hypersensitivity in an infraorbital nerve (ION) constricted TN model. We used adult Sprague Dawley rats subjected to infraorbital nerve (ION) constriction to mimic TN symptoms, with naive and sham rats serving as controls. For in vivo optogenetic manipulations, rats stereotaxically received AAV8-GFAP-eNpHR3.0-mCherry or AAV8-GFAP-mCherry at the trigeminal nucleus caudalis (TNC). Open field, von Frey, air puff, and acetone tests measured pain behavioral flexibility. In vivo thalamic recordings were obtained simultaneously with optogenetic manipulation in the TNC. Orofacial hyperalgesia and thalamic hyperexcitability were both accompanied by medullary astrocyte hyperactivity, marked by upregulated GFAP. The yellow laser-driven inhibition of TNC astrocytes markedly improved behavioral responses and regulated thalamic neuronal responses. Halorhodopsin-mediated inhibition in medullary astrocytes may modify the nociceptive input transmitted through the trigeminothalamic tract and pain perception. Taken together, these findings imply that this subpopulation in the TNC and its thalamic connections play a significant role in regulating the trigeminal pain circuitry, which might aid in the identification of new therapeutic measures in TN management.
Graphical Abstract
disperseNN2: a neural network for estimating dispersal distance from georeferenced polymorphism data
Authors: Smith Chris C. R. , Kern Andrew D. 
Published: October 2023

AbstractSpatial genetic variation is shaped in part by an organism’s dispersal ability. We present a deep learning tool, , for estimating the mean per-generation dispersal distance from georeferenced polymorphism data. Our neural network performs feature extraction on pairs of genotypes, and uses the geographic information that comes with each sample. These attributes led to outperform a state-of-the-art deep learning method that does not use explicit spatial information: the mean relative absolute error was reduced by 33% and 48% using sample sizes of 10 and 100 individuals, respectively. is particularly useful for non-model organisms or systems with sparse genomic resources, as it uses unphased, single nucleotide polymorphisms as its input. The software is open source and available from https://github.com/kr-colab/disperseNN2, with documentation located at https://dispersenn2.readthedocs.io/en/latest/.
Regulation of cashmere fineness traits by noncoding RNA in Jiangnan cashmere goats
Authors: Wu Cuiling , Xu Qin , Li Jianying , Qin Chongkai , Tulafu Hanikezi , Liu Wenna , Lu Qingwei , Zheng Wenxin , Fu Xuefeng 
Published: October 2023

Abstract
Background
Cashmere has long been used as the raw material for wool textiles. The diameter of the cashmere fibre determines its quality and economic value. However, the regulatory role of noncoding RNAs (ncRNAs) in cashmere fineness remains unclear, especially regarding the interaction between ncRNAs and coding RNAs.

Results
Transcriptome sequencing was used to identify the expression profiles of long noncoding RNAs (lncRNAs), circular RNAs (circRNAs) and microRNAs (miRNAs) in the skin tissues of Jiangnan cashmere goats with different cashmere fineness levels. Integration analysis of ncRNA and coding RNA was performed in combination with previous research results. The results showed that 16,437 lncRNAs, 2234 circRNAs, and 1322 miRNAs were identified in 8 skin samples of cashmere goats. A total of 403 differentially expressed (DE) lncRNAs, 62 DE circRNAs and 30 DE miRNAs were identified in the skin tissues of the fine groups (Fe) and coarse groups (Ce). We predicted the target gene of DE lncRNA, the target gene of DE miRNA and the host gene of DE circRNA. Based on functional annotation and enrichment analysis of target genes, we found that DE lncRNAs could be involved in regulating the fineness traits of cashmere. The most potential lncRNAs were MSTRG.42054.1, MSTRG.18602.3, and MSTRG.2199.13.

Conclusions
The data from this study enriched the cashmere goat noncoding RNA database and helped to supplement the annotation of the goat genome. The results provided a new direction for the breeding of cashmere characters.
In silico and computational analysis of zinc finger motif-associated homeodomain (ZF-HD) family genes in chilli (Capsicum annuum L)
Authors: Islam Md. Abir Ul , Nupur Juthy Abedin , Shafiq Muhammad , Ali Qurban , Sami Adnan , Shahid Muhammad Adnan 
Published: October 2023

AbstractZinc finger-homeodomain (ZHD) proteins are mostly expressed in plants and are involved in proper growth and development and minimizing biotic and abiotic stress. A recent study identified and characterized the ZHD gene family in chilli (Capsicum annuum L.) to determine their probable molecular function. ZHD genes with various physicochemical characteristics were discovered on twelve chromosomes in chilli. We separated ZHD proteins into two major groups using sequence alignment and phylogenetic analysis. These groups differ in gene structure, motif distribution, and a conserved ZHD and micro-zinc finger ZF domain. The majority of the CaZHDs genes are preserved, early duplication occurred recently, and significant pure selection took place throughout evolution, according to evolutionary study. According to expression profiling, the genes were found to be equally expressed in tissues above the ground, contribute to plant growth and development and provide tolerance to biotic and abiotic stress. This in silico analysis, taken as a whole, hypothesized that these genes perform distinct roles in molecular and phytohormone signaling processes, which may serve as a foundation for subsequent research into the roles of these genes in other crops.
Chromosome-level genome assemblies of Cutaneotrichosporon spp. (Trichosporonales, Basidiomycota) reveal imbalanced evolution between nucleotide sequences and chromosome synteny
Authors: Kobayashi Yuuki , Kayamori Ayane , Aoki Keita , Shiwa Yuh , Matsutani Minenosuke , Fujita Nobuyuki , Sugita Takashi , Iwasaki Wataru , Tanaka Naoto , Takashima Masako 
Published: October 2023

Abstract
Background
Since DNA information was first used in taxonomy, barcode sequences such as the internal transcribed spacer (ITS) region have greatly aided fungal identification; however, a barcode sequence alone is often insufficient. Thus, multi-gene- or whole-genome-based methods were developed. We previously isolated Basidiomycota yeasts classified in the Trichosporonales. Some strains were described as Cutaneotrichosporon cavernicola and C. spelunceum, whereas strain HIS471 remained unidentified. We analysed the genomes of these strains to elucidate their taxonomic relationship and genetic diversity.

Results
The long-read-based assembly resulted in chromosome-level draft genomes consisting of seven chromosomes and one mitochondrial genome. The genome of strain HIS471 has more than ten chromosome inversions or translocations compared to the type strain of C. cavernicola despite sharing identical ITS barcode sequences and displaying an average nucleotide identity (ANI) above 93%. Also, the chromosome synteny between C. cavernicola and the related species, C. spelunceum, showed significant rearrangements, whereas the ITS sequence identity exceeds 98.6% and the ANI is approximately 82%. Our results indicate that the relative evolutionary rates of barcode sequences, whole-genome nucleotide sequences, and chromosome synteny in Cutaneotrichosporon significantly differ from those in the model yeast Saccharomyces.

Conclusions
Our results revealed that the relative evolutionary rates of nucleotide sequences and chromosome synteny are different among fungal clades, likely because different clades have diverse mutation/repair rates and distinct selection pressures on their genomic sequences and syntenic structures. Because diverse syntenic structures can be a barrier to meiotic recombination and may lead to speciation, the non-linear relationships between nucleotide and synteny diversification indicate that sequence-level distances at the barcode or whole-genome level are not sufficient for delineating species boundaries.
Does the evolution of micromorphology accompany chromosomal changes on dysploid and polyploid levels in the Barnardia japonica complex (Hyacinthaceae)?
Authors: Kim Hyeonjin , Choi Bokyung , Lee Changyoung , Paik Jin-Hyub , Jang Chang-Gee , Weiss-Schneeweiss Hanna , Jang Tae-Soo 
Published: October 2023

Abstract
Background
Chromosome number and genome size changes via dysploidy and polyploidy accompany plant diversification and speciation. Such changes often impact also morphological characters. An excellent system to address the questions of how extensive and structured chromosomal changes within one species complex affect the phenotype is the monocot species complex of Barnardia japonica. This taxon contains two well established and distinct diploid cytotypes differing in base chromosome numbers (AA: x = 8, BB: x = 9) and their allopolyploid derivatives on several ploidy levels (from 3x to 6x). This extensive and structured genomic variation, however, is not mirrored by gross morphological differentiation.

Results
The current study aims to analyze the correlations between the changes of chromosome numbers and genome sizes with palynological and leaf micromorphological characters in diploids and selected allopolyploids of the B. japonica complex. The chromosome numbers varied from 2n = 16 and 18 (2n = 25 with the presence of supernumerary B chromosomes), and from 2n = 26 to 51 in polyploids on four different ploidy levels (3x, 4x, 5x, and 6x). Despite additive chromosome numbers compared to diploid parental cytotypes, all polyploid cytotypes have experienced genome downsizing. Analyses of leaf micromorphological characters did not reveal any diagnostic traits that could be specifically assigned to individual cytotypes. The variation of pollen grain sizes correlated positively with ploidy levels.

Conclusions
This study clearly demonstrates that karyotype and genome size differentiation does not have to be correlated with morphological differentiation of cytotypes.