bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combination chemotherapy (cyclophosphamide, doxorubicin, and vincristine with continuous‐infusion cisplatin and etoposide) and radiotherapy with stem cell support can be beneficial for adolescents and adults with estheisoneuroblastoma
Authors: Mishima Yuko , Nagasaki Eijiro , Terui Yasuhito , Irie Tetsuya , Takahashi Shunji , Ito Yoshinori , Oguchi Masahiko , Kawabata Kazuyoshi , Kamata Shinetsu , Hatake Kiyohiko 
Published: July 2004

AbstractBACKGROUNDAdolescent‐onset and adult‐onset esthesioneuroblastoma is a rare disease and is considered incurable. In many patients, local resection and radiation are chosen as clinical therapy with or without chemotherapy. It was reported previously that local resection and radiotherapy led to temporary remission and, in many patients, recurrent disease. Although combination with chemotherapy has been regarded as promising, an effective regimen has not been established. In the current study, the authors investigated the effect and tolerability of the combination of chemotherapy, radiotherapy, and peripheral blood stem cell transplantation (PBSCT).METHODSThe study population included 12 patients with adolescent‐onset and adult‐onset esthesioneuroblastoma classified as Kadish Stage A–D. The patients received two cycles of combination chemotherapy, which consisted of cyclophosphamide, doxorubicin, and vincristine (CAD) with continuous‐infusion cisplatin and etoposide (CVP). This was combined with radiotherapy with or without PBSCT.RESULTSNine of 12 patients (75%) obtained more than a partial response after only 2 cycles of chemotherapy. After radiation with or without PBSCT, six patients obtained a complete remission (CR). The longest survival was > 3 years. All patients who underwent PBSCT obtained a CR. The most severe side effects were loss of sodium and potassium induced by cisplatin‐related renal tubular distress. Those abnormalities were temporary, and all patients recovered.CONCLUSIONSThe chemotherapy regimen with CADO and CVP does not require continuation for a long time and is very effective and tolerable for patients with adolescent‐onset and adult‐onset esthesioneuroblastoma. The combination with radiotherapy and PBSCT may lead to a CR without facial disfigurement. In this report, the authors discuss the feasibility and efficacy of this multidisciplinary approach. Cancer 2004. © 2004 American Cancer Society.
LAMP‐1 and LAMP‐2, but not LAMP‐3, are reliable markers for activation‐induced secretion of human mast cells
Authors: Grützkau Andreas , Smorodchenko Alina , Lippert Undine , Kirchhof Loreen , Artuc Metin , Henz Beate M. 
Published: July 2004

AbstractBackgroundMast cells are resident tissue cells that induce anaphylactic reactions by rapidly releasing mediators after antigen‐mediated cross‐linking of immunoglobulin E receptors. In the similarly active peripheral blood basophilic leukocyte, lysosome‐associated membrane protein 3 (LAMP‐3; CD63) has been described as an activation marker, but LAMPs have not been investigated in normal tissue mast cells.MethodsIntra‐ and extracellular expressions of LAMP‐1 (CD107a), LAMP‐2 (CD107b), and LAMP‐3 (CD63) were analysed by flow cytometry, immunocytochemistry, and functional assays in unstimulated and stimulated leukemic human mast cell line 1 (HMC‐1) and skin mast cells.ResultsOn flow cytometry, all mast cells expressed LAMP‐3 at their cell membranes, whereas LAMP‐1 and LAMP‐2 were barely detectable (HMC‐1 cells) or expressed at low levels (<10% of skin mast cells). After fixation and permeabilisation, high intracellular levels of all three LAMPs were noted in both cell types. After stimulation, a rapid translocation of intracellular LAMPs to the cell membrane, with an associated release of histamine, leukotriene C4 and prostaglandin D2, was ascertained in skin mast cells only.ConclusionThese results show that LAMP‐1 and LAMP‐2 are activation markers for normal mast cells. The lack of LAMP translocation after activation of leukemic mast cells may be related to maturation or malignancy‐associated defects of these cells. © 2004 Wiley‐Liss, Inc.
NKT cell ligand α‐galactosylceramide blocks the induction of oral tolerance by triggering dendritic cell maturation
Authors: Chung Yeonseok , Chang Woo‐Sung , Kim Sanghee , Kang Chang‐Yuil 
Published: July 2004

AbstractNKT cells play contradictory roles in vivo, both regulating autoimmunity and activating immunity to intracellular pathogens and tumors. In this study, we studied the effect of NKT cell activation on the induction of systemic tolerance by oral administration of antigen. Administration of α‐galactosylceramide (αGC) at the time of oral ovalbumin (OVA) feeding completely blocked the OVA‐specific tolerance induced by both high‐ and low‐dose regimens in BALB/c mice. In the mesenteric lymph nodes (MLN) of αGC‐treated mice, the proliferation of OVA‐specific T cells was greater than that seen in the MLN of vehicle‐treated mice in vivo. The administration of αGC triggered the full maturation of mesenteric dendritic cells (DC), which were in turn responsible for the enhanced division of OVA‐specific T cells in vitro. To further determine whether the costimulation provided by DC in αGC‐treated mice was responsible for the reversal of oral tolerance in vivo, mice were given αGC together with anti‐CD80 and anti‐CD86 blocking Ab. OVA‐specific systemic tolerance was restored in mice given the blocking Ab, even when they simultaneously received αGC. Therefore, oral tolerance can be reversed via costimulation by DC that have been triggered to fully mature by the administration of αGC.
Potent T cell response to a class I‐binding 13‐mer viral epitope and the influence of HLA micropolymorphism in controlling epitope length
Authors: Green Kate J. , Miles John J. , Tellam Judy , van Zuylen Wendy J. M. , Connolly Geoff , Burrows Scott R. 
Published: July 2004

AbstractThe BZLF1 antigen of Epstein‐Barr virus includes three overlapping sequences of different lengths that conform to the binding motif of human leukocyte antigen (HLA) B*3501. These 9‐mer (56LPQGQLTAY64), 11‐mer (54EPLPQGQLTAY64), and 13‐mer (52LPEPLPQGQLTAY64) peptides all bound well to B*3501; however, the CTL response in individuals expressing this HLA allele was directed strongly and exclusively towards the 11‐mer peptide. In contrast, EBV‐exposed donors expressing HLA B*3503 showed no significant CTL response to these peptides because the single amino acid difference between B*3501 and B*3503 within the F pocket inhibited HLA binding by these peptides. The extraordinarily long 13‐mer peptide was the target for the CTL response in individuals expressing B*3508, which differs from B*3501 at a single position within the D pocket (B*3501, 156Leucine; B*3508, 156Arginine). This minor difference was shown to enhance binding of the 13‐mer peptide, presumably through a stabilizing interaction between the negatively charged glutamate at position 3 of the peptide and the positively charged arginine at HLA position 156. The 13‐mer epitope defined in this study represents the longest class I‐binding viral epitope identified to date as a minimal determinant. Furthermore, the potency of the response indicates that peptides of this length do not present a major structural barrier to CTL recognition.
New N,N‐Diisopropylcarbamato Complexes of Ruthenium(II) as Catalytic Precursors for Olefin Hydrogenation
Authors: Belli Dell'Amico Daniela , Calderazzo Fausto , Englert Ulli , Labella Luca , Marchetti Fabio , Specos Monica 
Published: July 2004

AbstractDuring the synthesis of [Ru(O2CNiPr2)2(PPh3)2] (1), the [RuCl2(PPh3)3]/NHiPr2/CO2 system produces the intermediates [NH2iPr2][Ru2Cl2(μ‐Cl)3(PPh3)4] (2) and [RuCl(O2CNiPr2)(PPh3)3] (3) which have been isolated and fully characterised. Compound 2 contains the dinuclear anionic triply chloride‐bridged ruthenium(II) species. Compound 3 is mononuclear, the octahedral ruthenium centre being coordinated to the bidentate carbamato ligand. The reactions of compounds 1 and 3 with dihydrogen have been studied at room temperature and atmospheric pressure with respect to their catalytic hydrogenation of 1‐octene. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Hydrothermal Synthesis, Structure and Magnetic Properties of a One‐Dimensional Iron Arsenate, 1∞[NH3(CH2)2NH(CH2)2NH3][Fe2F4(HAsO4)2]
Authors: Chakrabarti Sandip , Pati Swapan K. , Green Mark A. , Natarajan Srinivasan 
Published: July 2004

AbstractA novel one‐dimensional iron arsenate, 1∞[NH3(CH2)2NH(CH2)2NH3][Fe2F4(HAsO4)2], built up exclusively from SBU‐4 units (two octahedral units sharing an edge and connected through two tetrahedra) with Fe3+ ions in the high‐spin state has been synthesized by hydrothermal methods. Structure elucidation reveals that the compound is a two‐leg ladder with very weak antiferromagnetic exchanges along the chains. The magnetic susceptibility data show activated behaviour at T = 14 K, suggesting a finite spin gap between the nonmagnetic ground state and the excited triplet state. We have estimated the exchange parameters theoretically based on the S = 5/2 Majumdar−Ghosh model with frustrations and dimerisations. Although the magnetic gap is quite small, this is the first example, to the best of our knowledge, where the rungs with two S = 5/2 spins form strong singlet dimers. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Discrete Dinuclear Complexes and Two‐Dimensional Architectures from Bridging Polynitrile and Bipyrimidine (bpym) Ligands: Syntheses, Structures and Magnetic Properties of [M2(bpym)(dcne)4(H2O)2] (M = MnII, CoII) and [M2(bpym)(dcne)4(H2O)4]·2H2O (M = FeII, CuII) (dcne− = [(CN)2CC(O)OEt)]−)
Authors: Thétiot Franck , Triki Smail , Sala Pala Jean , Galán‐Mascarós José‐Ramón , Martínez‐Agudo José M. , Dunbar Kim R. 
Published: July 2004

AbstractOne‐pot reactions in aqueous solutions of the polynitrile anion dcne− {2,2‐dicyano‐1‐ethoxyethenolate = [(CN)2CC(O)OEt)]−] with the MII ions (M = Mn, Fe, Co, Cu) in the presence of bpym (2,2′‐bipyrimidine) afford the first mixed dcne/bpym compounds [M2(bpym)(dcne)4(H2O)2] (1: M = Mn; 2: M = Co) and [M2(bpym)(dcne)4(H2O)4]·2H2O (3: M = Fe; 4: M = Cu). The new compounds have been characterized by IR spectroscopy and X‐ray crystallography. Compounds 1 and 2 are isostructural, with each metal ion being located in an MN5O pseudo‐octahedral environment with three N atoms coming from three dcne− ligands, two nitrogen atoms from bpym and one oxygen atom from a water molecule. The extended structures of 1 and 2 are best described as dcne‐bridged zigzag chains of MII ions running along the [100] direction; connections of these chains in the [010] direction, by the bis(chelating) bpym ligand, afford 2D structures. Compounds 3 and 4 are isostructural, and consist of discrete dinuclear units involving MN4O2 octahedrally coordinated MII ions bridged by bis(bidentate) 2,2′‐bipyrimidine and terminal dcne ligands. Magnetic measurements for the 2D compounds 1 and 2 exhibit maxima in the χm vs. T plots (at about 4.5 K for 1 and about 20 K for 2) which are characteristic of weak antiferromagnetic exchange interactions between the high‐spin metal centres. While the dinuclear iron complex 3 presents a similar behaviour (maximum in χm vs. T plot at 12 K), the antiferromagnetic exchange interactions are stronger in the copper complex 4. Fits of magnetic data for compounds 1, 3 and 4 with appropriate models led to J values of −0.6, −1.5 and −99.0 cm−1 respectively. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Stabilization of a Copper(II) Complex by Hydrogen Bonds between the Axial Tetrafluoroborate Anion and the Ammonium Group from the Coordinated Cationic Ligand Bis(imidazol‐2‐yl)methyl(isopropyl)ammonium
Authors: Song Yufei , Massera Chiara , Gamez Patrick , Manotti Lanfredi Anna Maria , Reedijk Jan 
Published: July 2004

AbstractA new mononuclear copper complex [Cu(HIm‐iPr)2(BF4)2](BF4)2·2CH3OH {where Im‐iPr is [bis(imidazol‐2‐yl)methyl](isopropyl)amine} has been obtained by diethyl ether diffusion into a methanol solution of Im‐iPr and Cu(BF4)2. The complex has been characterized by X‐ray analysis, UV/Vis, IR and EPR spectroscopy. Its crystal structure is built up by centrosymmetric [Cu(HIm‐iPr)2(BF4)2]2+ complex cations, BF4− counterions and methanol lattice molecules. In the cation, the Cu atom exhibits a distorted octahedral CuN4F2 chromophore coordinated by two axial BF4− ions and by four equatorial imidazole nitrogen atoms from two cationic HIm‐iPr ligands. The complex is stabilized by two intramolecular pseudo‐chelate hydrogen bonds between the axial BF4− ions and the ammonium nitrogen atoms of the HIm‐iPr ligand [N5···F8 = 2.761(5) Å]. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Microwave‐Assisted Generation of Lanthanide(II) Halides in THF and Simple Quantitative Determination
Authors: Dahlén Anders , Hilmersson Göran 
Published: July 2004

AbstractLanthanide(II) reagents (SmI2, SmBr2, YbI2, EuI2) have been prepared rapidly in high yields using microwave‐assisted heating. Samarium diiodide is obtained as a saturated solution in THF (0.13M) within 5 min and ytterbium diiodide (0.065M) after 45 min. A simple method for quantitative determination of LnX2in THF is also described by utilizing the instantaneous reaction with ketones in the presence of an amine and water. This method establishes the concentration of the active single‐electron‐donor species, i.e. Ln2+. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2004)
Cover Picture: Novel Phosphovanadate Layered Structure Assembled from a Tetrametallic Cubane‐Like VV Cluster (Eur. J. Inorg. Chem. 15/2004)
Authors: Shi Fa‐Nian , Paz Filipe A. Almeida , Rocha João , Klinowski Jacek , Trindade Tito 
Published: July 2004

AbstractThe cover picture shows a view in perspective of a novel phosphovanadate layered structure intercalated by 4,4′‐bipyridinium cations which act as hydrogen‐bonded pillars between adjacent layers. These inorganic layers are constructed by an unprecendented secondary tetrametallic VV building unit exhibiting a distorted cubane‐type structural motif. Details are discussed in the article by J. Rocha et al. on p. 3031 ff.
Signiﬁcance of changed climatic factors on essential oil composition of Echinacea purpurea under subtropical conditions
Authors: Thappa R. K. , Bakshi S. K. , Dhar P. L. , Agarwal S. G. , Kitchlu S. , Kaul M. K. , Suri K. A. 
Published: July 2004

AbstractVariation pattern in the composition of essential oil, hydrodistilled from the over‐matured ﬂower heads of Echinacea purpurea (L.) Moench under subtropical climate was characterized by GC–MS and GC analysis. The plants ﬂower from June till December and climatic factors such as temperature and humidity were found to affect both the content and the composition of the essential oil. Copyright © 2004 John Wiley & Sons, Ltd.
Essential oil composition of Satureja spicigera (C. Koch) Boiss. from Iran
Authors: Seﬁdkon F. , Jamzad Z. 
Published: July 2004

AbstractHydro‐distilled volatile oil from the aerial parts of Satureja spicigera (C. Koch) Boiss. was obtained at yield of 3.82% w/w based on dry weight. The oil was analyzed by a combination of GC and GC/MS. Forty‐eight components were identiﬁed that approximately constitute 96% of the oil. The main constituents of the essential oil were thymol (35.1%), p‐cymene (22.1%), γ‐terpinene (13.7%) and carvacrol (4.0%). Copyright © 2004 John Wiley & Sons, Ltd.
The essential oil of Tagetes erecta L. occurring in Iran
Authors: Seﬁdkon F. , Salehyar S. , Mirza M. , Dabiri M. 
Published: July 2004

AbstractHydro‐distilled volatile oils from the leaves and stems and the ﬂowers of Tagetes erecta L. were analyzed by a combination of GC and GC/MS. Thirty‐three components in leaf and stem oil and 34 components in ﬂower oil were identiﬁed. The main characterized constituents were β‐caryophyllene (8.5 and 35.2%), terpinolene (18.4 and 6.3%), (E)‐ocimenone (12.6 and 9.8%), (Z)‐β‐ocimene (10.4 and 13.7%), piperitenone (10.4 and 2.6%), (Z)‐ocimenone (5.5 and 7.7%) and limonene (6.2 and 2.5%) in leaf and stem and ﬂower oils respectively. Copyright © 2004 John Wiley & Sons, Ltd.
Ein Protein erscheint in einem neuen Licht
Authors: Herzog Volker , Kirfel Gregor , Schmitz Anton , Tikkanen Ritva 
Published: July 2004

AbstractFür die Alzheimersche Krankheit könnte auch das Protein sAPP mitverantwortlich sein. Welche Aufgaben dieses Protein im menschlichen Organismus übernimmt, ist deshalb nicht nur für den Grundlagenforscher aufschlussreich
Der Computer und die Operation Schädeldecke
Authors: Eufinger Harald , Weihe Stephan , Wehmöller Michael 
Published: July 2004

AbstractUm Lücken in der Schädeldecke zu schließen, werden Titanimplantate passgenau eingesetzt. Dabei können computergestützte Verfahren und Roboter helfen, die in enger Kooperation zwischen Medizinern und Ingenieuren entwickelt werden
Von Schlickwatt, Mischwatt und Sandwatt
Authors: Rullkötter Jürgen 
Published: July 2004

AbstractAusgedehnte Wattflächen sind das Ergebnis der Gezeiten in verschiedenen Klimazonen der Erde. Am Beispiel der südlichen Nordsee untersuchen Wissenschaftler, wie ökologische Prozesse im bedrohten „Lebensraum Wattenmeer”︁ ablaufen
Transposon vectors for gene‐trap insertional mutagenesis in vertebrates
Authors: Clark Karl J. , Geurts Aron M. , Bell Jason B. , Hackett Perry B. 
Published: July 2004

AbstractThe function of most vertebrate genes remains unknown or uncertain. Insertional mutagenesis offers one approach to identify and understand the function of these genes. Transposons have been used successfully in lower organisms and plants for insertional mutagenesis, but until activation of the Sleeping Beauty (SB) transposon system, there was no indication of active DNA‐based transposons in vertebrates. Investigator‐driven insertional mutagenesis in vertebrates has relied on retroviral insertions or selection of low‐frequency integration of naked DNA in ES cell lines. We have combined the highly active SB transposon with gene‐trapping technology to demonstrate that transposon traps can be used for insertional mutagenesis screens in vertebrates. In our studies about one‐fourth of the trap insertions appear to be in transcriptional units, a rate that is commensurate with random integration. We show that gene‐traps coupled to a fluorescent protein reporter gene can be used to detect insertions into genes active in specific cells of living zebrafish embryos, supporting use of our transposon traps for high‐throughput functional genomic screens in vertebrates. genesis 39:225–233, 2004. © 2004 Wiley‐Liss, Inc.
Refining GAL4‐driven transgene expression in Drosophila with a GAL80 enhancer‐trap
Authors: Suster Maximiliano L. , Seugnet Laurent , Bate Michael , Sokolowski Marla B. 
Published: July 2004

AbstractWe constructed an enhancer‐trap element, P{GAL80}, that encodes the yeast GAL80 repressor to refine expression of transgenes driven by the binary GAL4/UAS system. GAL80 blocks GAL4 activity by binding to its transcriptional activation domain. We screened GAL80 enhancer‐traps for repression of GAL4‐induced green fluorescent protein (GFP) in the intact larval nervous system. We selected one line that repressed GFP in a large set of cholinergic neurons. This line was used to refine GFP expression from a set of over 200 neurons to a subset of 20 neurons in a preselected GAL4 line. Expression of tetanus neurotoxin, a potent blocker of neurotransmitter release, in these 20 neurons reproduced an aberrant larval turning behavior previously assigned to the parental set of 200 neurons. Our results suggest that targeted GAL80 expression could become a useful means of spatially refining transgene expression in Drosophila. genesis 39:240–245, 2004. © 2004 Wiley‐Liss, Inc.
Localization of Swallow‐Green fluorescent protein in Drosophila oogenesis and implications for the role of Swallow in RNA localization
Authors: Stephenson Edwin C. 
Published: July 2004

AbstractThe localization of a hybrid protein composed of swallow and Green Fluorescent Protein (GFP) during Drosophila oogenesis is reported. I constructed a hybrid gene with GFP inserted into an internal position of swallow. This gene was integrated into the Drosophila genome and provides full swallow+ function, as assayed by the complete rescue of strong swallow mutants. Swallow‐GFP is localized at all points along the oocyte cortex from vitellogenic stages of oogenesis through the end of oogenesis. Higher concentrations of swallow‐GFP are present at the anterior oocyte cortex than at the lateral and posterior oocyte cortices at Stages 10 and 11, when bicoid and htsN4 mRNA transport from nurse cells and localization in the oocyte are most active. At Stage 9 and at Stages 12–14 swallow‐GFP is equally distributed at the anterior, lateral, and posterior oocyte cortices. The position of swallow‐GFP in vitellogenic stages is identical to the position of endogenous swallow protein determined by indirect immunofluorescence using an anti‐swallow antibody. At the oocyte cortex, swallow‐GFP is present in particulate structures that lie within or just internal to the dense cortical actin meshwork. These particles show little or no movement, suggesting that they are attached to or embedded in the oocyte cortex. These observations are most easily interpreted in the context of mRNA anchoring or microtubule organizing functions for the swallow protein. genesis 39:280–287, 2004. © 2004 Wiley‐Liss, Inc.
Repeat Exposure to Incremental Doses of Acetaminophen Provides Protection Against Acetaminophen–Induced Lethality in Mice: An Explanation for High Acetaminophen Dosage in Humans Without Hepatic Injury
Authors: Shayiq Rass M. , Roberts Dean W. , Rothstein Kenneth , Snawder John E. , Benson Wayne , Ma Xiang , Black Martin 
Published: July 2004

In studies designed to simulate a clinical observation in which an individual became tolerant to normally lethal doses of acetaminophen (APAP), mice were pretreated with increasing doses of APAP for 8 days and challenged on day 9 with normally supralethal doses of APAP. These animals developed minimal hepatotoxicity after a challenge dose with a fourfold increase in LD50 to 1,350 mg/kg. The pretreatment regimen resulted in hepatic changes including: centrilobular localization of 3–(cysteine–S–yl)APAP protein adducts, selective down–regulation of cytochrome P4502E1 (CYP2E1) and CYP1A2 that produced the toxic metabolite, N–acetyl–p–benzoquinone imine, higher levels of reduced glutathione (GSH), centrilobular inflammation, and a fourfold increase in hepatocellular proliferation. The protection against the lethal APAP doses afforded by pretreatment is secondary to these changes and to the associated regional shift in the bioactivation of the APAP challenge dose from centrilobular to periportal regions where CYP2E1 is not found, protective GSH is more abundant, and where cell–proliferative responses are better able to sustain repair. This shift in APAP bioactivation results in less–intense covalent binding that is more diffuse and spread uniformly throughout the hepatic lobe, most likely contributing to protection by delaying the early onset of liver injury that has been generally associated with centrilobular localization of the adducts. Intervention of APAP pretreatment-induced cell division in mice with colchicine left them resistant to a 500–mg/kg (normally lethal) dose of APAP, but unable to survive a 1,000–mg/kg APAP challenge dose. The data demonstrate multiple mechanistic components to the protection afforded by APAP pretreatment. Whereas metabolic and physiological changes not dependent on cell proliferation are adequate to protect against 500 mg/kg APAP, these changes plus a potentiated cell–proliferative response are necessary for protection against the supralethal 1,000–mg/kg APAP dose. Furthermore, the data document an uncoupling of the traditional association between covalent binding and toxicity, and suggest that the assessment of toxicity following repeated or chronic APAP exposure must consider altered drug interactions and parameters besides those historically used to assess acute APAP overdose.
(Hepatology 1999;29:451–463.)