bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural basis of ribosomal 30S subunit degradation by RNase R
Authors: Dimitrova-Paternoga Lyudmila , Kasvandik Sergo , Beckert Bertrand , Granneman Sander , Tenson Tanel , Wilson Daniel N. , Paternoga Helge 
Published: February 2024

AbstractProtein synthesis is a major energy-consuming process of the cell that requires the controlled production1–3 and turnover4,5 of ribosomes. Although the past few years have seen major advances in our understanding of ribosome biogenesis, structural insight into the degradation of ribosomes has been lacking. Here we present native structures of two distinct small ribosomal 30S subunit degradation intermediates associated with the 3′ to 5′ exonuclease ribonuclease R (RNase R). The structures reveal that RNase R binds at first to the 30S platform to facilitate the degradation of the functionally important anti-Shine–Dalgarno sequence and the decoding-site helix 44. RNase R then encounters a roadblock when it reaches the neck region of the 30S subunit, and this is overcome by a major structural rearrangement of the 30S head, involving the loss of ribosomal proteins. RNase R parallels this movement and relocates to the decoding site by using its N-terminal helix-turn-helix domain as an anchor. In vitro degradation assays suggest that head rearrangement poses a major kinetic barrier for RNase R, but also indicate that the enzyme alone is sufficient for complete degradation of 30S subunits. Collectively, our results provide a mechanistic basis for the degradation of 30S mediated by RNase R, and reveal that RNase R targets orphaned 30S subunits using a dynamic mechanism involving an anchored switching of binding sites.
Transforming a head direction signal into a goal-oriented steering command
Authors: Westeinde Elena A. , Kellogg Emily , Dawson Paul M. , Lu Jenny , Hamburg Lydia , Midler Benjamin , Druckmann Shaul , Wilson Rachel I. 
Published: February 2024

AbstractTo navigate, we must continuously estimate the direction we are headed in, and we must correct deviations from our goal1. Direction estimation is accomplished by ring attractor networks in the head direction system2,3. However, we do not fully understand how the sense of direction is used to guide action. Drosophila connectome analyses4,5 reveal three cell populations (PFL3R, PFL3L and PFL2) that connect the head direction system to the locomotor system. Here we use imaging, electrophysiology and chemogenetic stimulation during navigation to show how these populations function. Each population receives a shifted copy of the head direction vector, such that their three reference frames are shifted approximately 120° relative to each other. Each cell type then compares its own head direction vector with a common goal vector; specifically, it evaluates the congruence of these vectors via a nonlinear transformation. The output of all three cell populations is then combined to generate locomotor commands. PFL3R cells are recruited when the fly is oriented to the left of its goal, and their activity drives rightward turning; the reverse is true for PFL3L. Meanwhile, PFL2 cells increase steering speed, and are recruited when the fly is oriented far from its goal. PFL2 cells adaptively increase the strength of steering as directional error increases, effectively managing the tradeoff between speed and accuracy. Together, our results show how a map of space in the brain can be combined with an internal goal to generate action commands, via a transformation from world-centric coordinates to body-centric coordinates.
Telomere-to-telomere gapless genome assembly of the Chinese sea bass (Lateolabrax maculatus)
Authors: Sun Zhilong , Li Shuo , Liu Yuyan , Li Weijing , Liu Kaiqiang , Cao Xuebin , Lin Jiliang , Wang Hongyan , Wang Qian , Shao Changwei 
Published: February 2024

AbstractChinese sea bass (Lateolabrax maculatus) is a highly sought-after commercial seafood species in Asian regions due to its excellent nutritional value. With the rapid advancement of bioinformatics, higher standards for genome analysis compared to previously published reference genomes are now necessary. This study presents a gapless assembly of the Chinese sea bass genome, which has a length of 632.75 Mb. The sequences were assembled onto 24 chromosomes with a coverage of over 99% (626.61 Mb), and telomeres were detected on 34 chromosome ends. Analysis using Merqury indicated a high level of accuracy, with an average consensus quality value of 54.25. The ONT ultralong and PacBio HiFi data were aligned with the assembly using minimap2, resulting in a mapping rate of 99.9%. The study also identified repeating elements in 20.90% (132.25 Mb) of the genome and inferred 22,014 protein-coding genes. These results establish meaningful groundwork for exploring the evolution of the Chinese sea bass genome and advancing molecular breeding techniques.
Chromosome level genome assembly of the Etruscan shrew Suncus etruscus
Authors: Bukhman Yury V. , Meyer Susanne , Chu Li-Fang , Abueg Linelle , Antosiewicz-Bourget Jessica , Balacco Jennifer , Brecht Michael , Dinatale Erica , Fedrigo Olivier , Formenti Giulio , Fungtammasan Arkarachai , Giri Swagarika Jaharlal , Hiller Michael , Howe Kerstin , Kihara Daisuke , Mamott Daniel , Mountcastle Jacquelyn , Pelan Sarah , Rabbani Keon , Sims Ying , Tracey Alan , Wood Jonathan M. D. , Jarvis Erich D. , Thomson James A. , Chaisson Mark J. P. , Stewart Ron 
Published: February 2024

AbstractSuncus etruscus is one of the world’s smallest mammals, with an average body mass of about 2 grams. The Etruscan shrew’s small body is accompanied by a very high energy demand and numerous metabolic adaptations. Here we report a chromosome-level genome assembly using PacBio long read sequencing, 10X Genomics linked short reads, optical mapping, and Hi-C linked reads. The assembly is partially phased, with the 2.472 Gbp primary pseudohaplotype and 1.515 Gbp alternate. We manually curated the primary assembly and identified 22 chromosomes, including X and Y sex chromosomes. The NCBI genome annotation pipeline identified 39,091 genes, 19,819 of them protein-coding. We also identified segmental duplications, inferred GO term annotations, and computed orthologs of human and mouse genes. This reference-quality genome will be an important resource for research on mammalian development, metabolism, and body size control.
Correlations between ADL in patients with SCI and caregiver burden, quality of life, and presenteeism in South Korea
Authors: Kim Sung Shin , Kim On Yoo , Kim Sun Hong , Heo Jae Eun , Ho Seung Hee , Kim Ju Hee , Bae Young-Hyeon 
Published: February 2024

AbstractThe correlations between activities of daily living (ADL) among patients with spinal cord injury (SCI) and their caregivers’ burden, quality of life (QoL), and presenteeism was investigated. Participants included outpatients and inpatients with SCI at a rehabilitation center and their caregivers, recruited between March 2020 and April 2021. Eighty-seven valid responses were analysed using independent t-tests and Pearson’s correlations. There was a difference in caregiver burden according to patients’ ADL performance. QoL was negatively correlated with caregiver burden and presenteeism. Caregiver burden and presenteeism were positively correlated. Social support can improve caregivers’ QoL and reduce caregiver burden and presenteeism-induced work impairment.
Memory type Max-EWMA control chart for the Weibull process under the Bayesian theory
Authors: Noor-ul-Amin Muhammad , Khan Imad , Iqbal Javed , Rasheed Zahid , Ismail Emad A. A. , Ahmad Bakhtyar 
Published: February 2024

AbstractThe simultaneous monitoring of both process mean and dispersion, particularly in normal processes, has garnered significant attention within the field. In this article, we present a new Bayesian Max-EWMA control chart that is intended to track a non-normal process mean and dispersion simultaneously. This is accomplished through the utilization of the inverse response function, especially in cases where the procedure follows a Weibull distribution. We used the average run length (ARL) and the standard deviation of run length (SDRL) to assess the efficacy of our suggested control chart. Next, we contrast our suggested control chart's performance with an already-existing Max-EWMA control chart. Our results show that compared to the control chart under consideration, the proposed control chart exhibits a higher degree of sensitivity. Finally, we present a useful case study centered around the hard-bake process in the semiconductor manufacturing sector to demonstrate the performance of our Bayesian Max-EWMA control chart under different Loss Functions (LFs) for a Weibull process. The case study highlights how flexible the chart is to various situations. Our results offer strong proof of the outstanding ability of the Bayesian Max-EWMA control chart to quickly identify out-of-control signals during the hard-bake procedure. This in turn significantly contributes to the enhancement of process monitoring and quality control.
Monitoring underwater volcano degassing using fiber-optic sensing
Authors: Caudron Corentin , Miao Yaolin , Spica Zack J. , Wollin Christopher , Haberland Christian , Jousset Philippe , Yates Alexander , Vandemeulebrouck Jean , Schmidt Bernd , Krawczyk Charlotte , Dahm Torsten 
Published: February 2024

AbstractContinuous monitoring of volcanic gas emissions is crucial for understanding volcanic activity and potential eruptions. However, emissions of volcanic gases underwater are infrequently studied or quantified. This study explores the potential of Distributed Acoustic Sensing (DAS) technology to monitor underwater volcanic degassing. DAS converts fiber-optic cables into high-resolution vibration recording arrays, providing measurements at unprecedented spatio-temporal resolution. We conducted an experiment at Laacher See volcano in Germany, immersing a fiber-optic cable in the lake and interrogating it with a DAS system. We detected and analyzed numerous acoustic signals that we associated with bubble emissions in different lake areas. Three types of text-book bubbles exhibiting characteristic waveforms are all found from our detections, indicating different nucleation processes and bubble sizes. Using clustering algorithms, we classified bubble events into four distinct clusters based on their temporal and spectral characteristics. The temporal distribution of the events provided insights into the evolution of gas seepage patterns. This technology has the potential to revolutionize underwater degassing monitoring and provide valuable information for studying volcanic processes and estimating gas emissions. Furthermore, DAS can be applied to other applications, such as monitoring underwater carbon capture and storage operations or methane leaks associated with climate change.
Synthesis, docking, MD simulation, ADMET, drug likeness, and DFT studies of novel furo[2,3-b]indol-3a-ol as promising Cyclin-dependent kinase 2 inhibitors
Authors: Gheidari Davood , Mehrdad Morteza , Bayat Mohammad 
Published: February 2024

AbstractA new series of furo[2,3-b]indol-3a-ol derivatives was synthesized to investigate their potential as inhibitors of the Cyclin-dependent kinase 2 (CDK2) enzyme. CDK2 is a serine/threonine protein kinase belonging to a family of kinases involved in the control of the cell cycle. Based on results from clinical studies, it has been shown that overexpression of CDK2 may play a role in the development of cancer. In order to discover highly effective derivatives, a process of in silico screening was carried out. The obtained results revealed that compound 3f. had excellent binding energies. In this study, in silico screening was used to investigate protein–ligand interactions and assess the stability of the most favorable conformation. The methods utilized included molecular docking, density functional theory (DFT) calculations using the B3LYP/6-31++G(d,p) basis set in the gas phase, molecular dynamic (MD) simulation, as well as the evaluation of drug-likeness scores. The pharmacokinetic and drug-likeness properties of the novel furo[2,3-b]indol-3a-ol derivatives suggest that these compounds have the potential to be considered viable candidates for future development as anticancer drugs.
Efficient CO2 adsorption using chitosan, graphene oxide, and zinc oxide composite
Authors: Fathalian Farnoush , Moghadamzadeh Hamidreza , Hemmati Alireza , Ghaemi Ahad 
Published: February 2024

AbstractThis study was deeply focused on developing a novel CTS/GO/ZnO composite as an efficient adsorbent for CO2 adsorption process. To do so, design of experiment (DOE) was done based on RSM-BBD technique and according to the DOE runs, various CTS/GO/ZnO samples were synthesized with different GO loading (in the range of 0 wt% to 20 wt%) and different ZnO nanoparticle’s loading (in the range of 0 wt% to 20 wt%). A volumetric adsorption setup was used to investigate the effect of temperature (in the range of 25–65 °C) and pressure (in the range of 1–9 bar) on the obtained samples CO2 uptake capability. A quadratic model was developed based on the RSM-BBD method to predict the CO2 adsorption capacity of the composite sample within design space. In addition, CO2 adsorption process optimization was conducted and the optimum values of the GO, ZnO, temperature, and pressure were obtained around 23.8 wt%, 18.2 wt%, 30.1 °C, and 8.6 bar, respectively, with the highest CO2 uptake capacity of 470.43 mg/g. Moreover, isotherm and kinetic modeling of the CO2 uptake process were conducted and the Freundlich model (R2 = 0.99) and fractional order model (R2 = 0.99) were obtained as the most appropriate isotherm and kinetic models, respectively. Also, thermodynamic analysis of the adsorption was done and the ∆H°, ∆S°, and ∆G° values were obtained around − 19.121 kJ/mol, − 0.032 kJ/mol K, and − 9.608 kJ/mol, respectively, indicating exothermic, spontaneously, and physically adsorption of the CO2 molecules on the CTS/GO/ZnO composite’s surface. Finally, a renewability study was conducted and a minor loss in the CO2 adsorption efficiency of about 4.35% was obtained after ten cycles, demonstrating the resulting adsorbent has good performance and robustness for industrial CO2 capture purposes.
Collusion risk in corporate networks
Authors: Villamil Isabela , Kertész János , Fazekas Mihály 
Published: February 2024

AbstractCollusion among economic operators increases prices, reduces product quality, and hinders innovation. Structural links can affect the incentive and ability of firms to behave competitively by facilitating collusion. We use a network-based approach to study the relationship between ownership links and bidding behavior in procurement markets. We build temporal multiplex networks based on firms’ ownership and co-bidding ties to find network measures that may signal collusion risk. We test four network measures, two at market-level (density and average harmonic closeness centrality) and two at firm-level (degree centrality and harmonic closeness centrality). Using data on public procurement contracts awarded in Sweden from 2010 to 2015, we found higher incidence of single bidding in markets that are more closely related through ownership links. Missing bidders are also more likely in these markets. Single bidding and missing bidders may indicate the presence of collusive arrangements such as of bid suppression or rotation. For the firm-level analyses, our results showed a positive relationship between winning probability and centrality in the ownership network. A similar result was obtained for cut-point position, indicating that firms that are more closely connected to other firms through ownership links have a more important position in the co-bidding network and are also more likely to win contracts.
A convolutional neural network to identify mosquito species (Diptera: Culicidae) of the genus Aedes by wing images
Authors: Sauer Felix G. , Werny Moritz , Nolte Kristopher , Villacañas de Castro Carmen , Becker Norbert , Kiel Ellen , Lühken Renke 
Published: February 2024

AbstractAccurate species identification is crucial to assess the medical relevance of a mosquito specimen, but requires intensive experience of the observers and well-equipped laboratories. In this proof-of-concept study, we developed a convolutional neural network (CNN) to identify seven Aedes species by wing images, only. While previous studies used images of the whole mosquito body, the nearly two-dimensional wings may facilitate standardized image capture and reduce the complexity of the CNN implementation. Mosquitoes were sampled from different sites in Germany. Their wings were mounted and photographed with a professional stereomicroscope. The data set consisted of 1155 wing images from seven Aedes species as well as 554 wings from different non-Aedes mosquitoes. A CNN was trained to differentiate between Aedes and non-Aedes mosquitoes and to classify the seven Aedes species based on grayscale and RGB images. Image processing, data augmentation, training, validation and testing were conducted in python using deep-learning framework PyTorch. Our best-performing CNN configuration achieved a macro F1 score of 99% to discriminate Aedes from non-Aedes mosquito species. The mean macro F1 score to predict the Aedes species was 90% for grayscale images and 91% for RGB images. In conclusion, wing images are sufficient to identify mosquito species by CNNs.
Early sleep after action observation plus motor imagery improves gait and balance abilities in older adults
Authors: Temporiti Federico , Galbiati Elena , Bianchi Francesco , Bianchi Anna Maria , Galli Manuela , Gatti Roberto 
Published: February 2024

AbstractAction observation plus motor imagery (AOMI) is a rehabilitative approach to improve gait and balance performance. However, limited benefits have been reported in older adults. Early sleep after motor practice represents a strategy to enhance the consolidation of trained skills. Here, we investigated the effects of AOMI followed by early sleep on gait and balance performance in older adults. Forty-five older adults (mean age: 70.4 ± 5.2 years) were randomized into three groups performing a 3-week training. Specifically, AOMI-sleep and AOMI-control groups underwent observation and motor imagery of gait and balance tasks between 8:00 and 10:00 p.m. or between 8:00 and 10:00 a.m. respectively, whereas Control group observed landscape video-clips. Participants were assessed for gait performance, static and dynamic balance and fear of falling before and after training and at 1-month follow-up. The results revealed that early sleep after AOMI training sessions improved gait and balance abilities in older adults compared to AOMI-control and Control groups. Furthermore, these benefits were retained at 1-month after the training end. These findings suggested that early sleep after AOMI may represent a safe and easy-applicable intervention to minimize the functional decay in older adults.
Exploring the paradoxical nature of cold temperature mortality in Europe
Authors: Walkowiak Marcin Piotr , Walkowiak Dariusz , Walkowiak Jarosław 
Published: February 2024

AbstractWhile low winter temperatures are associated with increased mortality, this phenomenon has been suggested to be most severe in regions with seemingly mild winters. The study aimed to establish a temperature-based formula that could elucidate the previously ambiguous regional differences in vulnerability to low temperature. European weekly mortality data (2000–2019) were matched with meteorological data to determine for each region vulnerability to temperature decrease and the optimal temperature with lowest mortality. Regression models were developed to generalize and explain these findings considering regional temperature characteristics. Optimal temperature could be predicted based on local average summer temperature (R2 = 85.6%). Regional vulnerability to temperature decrease could be explained by combination of winter and summer temperatures (R2 = 86.1%). Regions with warm winters and cold summers showed the highest vulnerability to decrease of temperature during winter. Contrary to theories about economic disparities Eastern Europe exhibited resistance comparable to Scandinavia. The southern edges of Europe demonstrated serious low temperature vulnerability to decreased temperatures, even if temperature was relatively high around 20 °C. This suggests that the observed connection primarily reflects the modulation of the length of respiratory virus infection seasons by climate conditions, counterbalanced by varying levels of acquired immunity and the presence of heatwaves eliminating the most frail individuals. Thus, relatively low vulnerability and a flat mortality cycle in countries with harsh climates paradoxically imply the presence of threats throughout the whole year.
Optimizing dynamic economic dispatch through an enhanced Cheetah-inspired algorithm for integrated renewable energy and demand-side management
Authors: Nagarajan Karthik , Rajagopalan Arul , Bajaj Mohit , Sitharthan R. , Dost Mohammadi Shir Ahmad , Blazek Vojtech 
Published: February 2024

AbstractThis study presents the Enhanced Cheetah Optimizer Algorithm (ECOA) designed to tackle the intricate real-world challenges of dynamic economic dispatch (DED). These complexities encompass demand-side management (DSM), integration of non-conventional energy sources, and the utilization of pumped-storage hydroelectric units. Acknowledging the variability of solar and wind energy sources and the existence of a pumped-storage hydroelectric system, this study integrates a solar-wind-thermal energy system. The DSM program not only enhances power grid security but also lowers operational costs. The research addresses the DED problem with and without DSM implementation to analyze its impact. Demonstrating effectiveness on two test systems, the suggested method's efficacy is showcased. The recommended method's simulation results have been compared to those obtained using Cheetah Optimizer Algorithm (COA) and Grey Wolf Optimizer. The optimization results indicate that, for both the 10-unit and 20-unit systems, the proposed ECOA algorithm achieves savings of 0.24% and 0.43%, respectively, in operation costs when Dynamic Economic Dispatch is conducted with Demand-Side Management (DSM). This underscores the advantageous capability of DSM in minimizing costs and enhancing the economic efficiency of the power systems. Our ECOA has greater adaptability and reliability, making it a promising solution for addressing multi-objective energy management difficulties within microgrids, particularly when demand response mechanisms are incorporated. Furthermore, the suggested ECOA has the ability to elucidate the multi-objective dynamic optimal power flow problem in IEEE standard test systems, particularly when electric vehicles and renewable energy sources are integrated.