bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effectiveness of glucocorticoid treatment in post-COVID-19 pulmonary involvement
Authors: Mizera Jan , Genzor Samuel , Sova Milan , Stanke Ladislav , Burget Radim , Jakubec Petr , Vykopal Martin , Pobeha Pavol , Zapletalová Jana 
Published: February 2024

Abstract
Rationale
Persistent respiratory symptoms following Coronavirus Disease 2019 (COVID-19) are associated with residual radiological changes in lung parenchyma, with a risk of development into lung fibrosis, and with impaired pulmonary function. Previous studies hinted at the possible efficacy of corticosteroids (CS) in facilitating the resolution of post-COVID residual changes in the lungs, but the available data is limited.

Aim
To evaluate the effects of CS treatment in post-COVID respiratory syndrome patients.

Patients and methods
Post-COVID patients were recruited into a prospective single-center observational study and scheduled for an initial (V1) and follow-up visit (V2) at the Department of Respiratory Medicine and Tuberculosis, University Hospital Olomouc, comprising of pulmonary function testing, chest x-ray, and complex clinical examination. The decision to administer CS or maintain watchful waiting (WW) was in line with Czech national guidelines.

Results
The study involved 2729 COVID-19 survivors (45.7% male; mean age: 54.6). From 2026 patients with complete V1 data, 131 patients were indicated for CS therapy. These patients showed significantly worse radiological and functional impairment at V1. Mean initial dose was 27.6 mg (SD ± 10,64), and the mean duration of CS therapy was 13.3 weeks (SD ± 10,06). Following therapy, significantly better improvement of static lung volumes and transfer factor for carbon monoxide (DLCO), and significantly better rates of good or complete radiological and subjective improvement were observed in the CS group compared to controls with available follow-up data (n = 894).

Conclusion
Better improvement of pulmonary function, radiological findings and subjective symptoms were observed in patients CS compared to watchful waiting. Our findings suggest that glucocorticoid therapy could benefit selected patients with persistent dyspnea, significant radiological changes, and decreased DLCO.
Liver and mucous secretion enzymatic biomarkers of Eobania vermiculata treated with some newly synthesized acrylamide derivatives
Authors: Emara Esam M. , El-Sawaf Maher A. , Khalifa Rasha F. 
Published: February 2024

Abstract
Background
Acrylamide derivatives have a potential biological activity as well as acting as precursors in many organic syntheses. Moreover, acrylamides and their derivatives cause convulsions and diffused damage to different sections of the nervous system of infected animals. Novel copper and zinc chelates originated from (E)-3-(4-bromophenyl)-2-cyanoacrylamide (L1), and (E)-2-cyano-3-(4-nitrophenyl)acrylamide (L2) were prepared, and their chemical skeletons were identified by infrared and mass spectra. The obtained compounds were screened in vitro against the brown garden snail, Eobania vermiculata using the contact method along 72 h. Stock solutions of tested compounds were prepared utilizing distilled water and DMSO mixture, and four concentrations of each compound were prepared (50, 150, 250 and 350 ppm). Eobania vermiculata snails were treated with LC50 concentrations of prepared compounds for 3 days, and live snails were used to estimate the level of some liver and mucous secretion enzymatic biomarkers: transaminases enzymes (Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST)), Total Protein content (TP), Acid Phosphatases (ACP) and Alkaline Phosphatase (ALP).

Results
The results demonstrated that the examined compounds have a relatively toxic effect toward the screened species. Zinc complexes displayed a higher toxicity than copper ones. The results authenticated considerable high effects of the synthesized compounds on investigated enzymes.

Conclusions
The promising effects of Cu(II), Zn(II) complexes (1, 2) on stimulating the mucous secretion of tested snails are clear through the elevated levels of ALP and ACP enzymes of treated snails. The enhancement or reduction of AST, ALT level and TP content of treated snails demonstrated the effects of prepared compounds on liver functions of these species.
From implementation to reflection: exploring faculty experiences in a curriculum-focused FOLC through multi-case analysis
Authors: More Tamar , Goldberg Fred , Basir Mo , Maier Steven , Price Edward 
Published: February 2024

AbstractFaculty development and support play an important role in improving undergraduate STEM education. Learning communities, including Faculty Online Learning Communities (FOLCs), can be a valuable resource for faculty seeking professional growth. This multi-case study examined how faculty gained value through participation in a curriculum-focused FOLC. Adopting a community of practice perspective, the value creation framework developed by Wenger, et al., was used to examine cycles of value creation for the five case study participants and identify key ways in which participation in the FOLC supported value creation.All five case study participants shared comprehensive stories about how they gained value through participation in FOLC activities over multiple years. All initiated their value creation stories by describing pedagogical approaches they were unhappy about. As their stories unfolded, they described how they implemented new and innovative strategies to address their concerns, leading to outcomes consistent with the original pedagogical goals of the learning community. Two faculty described how the community helped them see new leadership roles for themselves within their departments, outcomes that were not anticipated at the initiation of the FOLC. Other participants spoke of a decreased feeling of professional isolation. Several features of the FOLC emerged as crucial factors in value creation, including structural elements such as regular meetings, a shared curricular focus, and leadership and networking opportunities. Importantly, study participants identified extended participation in the community and continuing feedback from multiple perspectives as drivers of reflection and growth.These findings suggest that while short term participation in a FOLC or other faculty community may help achieve more prescribed outcomes, longer term participation supports a more emergent approach to faculty development. This research offers insights for faculty, curriculum developers, administrators, and others interested in promoting faculty development.
A structural equation modeling approach to understand users' perceptions of app-based CNG auto-rickshaw services in Dhaka City
Authors: Salehin Md Sakibus , Jubailee Tahia , Sourav Md. Mohaimenul Islam , Afnanin Sayma , Muniruzzaman Shah Md 
Published: February 2024

AbstractThe use of CNG auto-rickshaws is increasing in Dhaka, the capital of Bangladesh. CNG auto rickshaws are a crucial component of the transportation system of Dhaka City. These offer the dwellers a quick and affordable way to navigate the city’s congested streets. This study aims to determine how commuters in Dhaka City perceive the quality of app-based CNG auto rickshaw service. A structured questionnaire is developed based on the users’ reported attitudes and perceived risks. In order to comprehend how users feel about an app-based CNG auto rickshaw service, collected data were used to create a Structural Equation (SE) model. The responses are also evaluated using the Relative Importance Index (RII) method, which validates the SE model. The study shows that women’s safety and better safety experience are the most significant parameters while accepting app-based CNG auto rickshaw services. The results of this study will help the service providers involved in this operation better understand the best course of action to be taken in order to provide better service to commuters.
Machine Learning Predicts Oxaliplatin Benefit in Early Colon Cancer
Authors: Chen Lujia , Wang Ying , Cai Chunhui , Ding Ying , Kim Rim S. , Lipchik Corey , Gavin Patrick G. , Yothers Greg , Allegra Carmen J. , Petrelli Nicholas J. , Suga Jennifer Marie , Hopkins Judith O. , Saito Naoyuki G. , Evans Terry , Jujjavarapu Srinivas , Wolmark Norman , Lucas Peter C. , Paik Soonmyung , Sun Min , Pogue-Geile Katherine L. , Lu Xinghua 
Published: February 2024

PURPOSE A combination of fluorouracil, leucovorin, and oxaliplatin (FOLFOX) is the standard for adjuvant therapy of resected early-stage colon cancer (CC). Oxaliplatin leads to lasting and disabling neurotoxicity. Reserving the regimen for patients who benefit from oxaliplatin would maximize efficacy and minimize unnecessary adverse side effects. METHODS We trained a new machine learning model, referred to as the colon oxaliplatin signature (COLOXIS) model, for predicting response to oxaliplatin-containing regimens. We examined whether COLOXIS was predictive of oxaliplatin benefits in the CC adjuvant setting among 1,065 patients treated with 5-fluorouracil plus leucovorin (FULV; n = 421) or FULV + oxaliplatin (FOLFOX; n = 644) from NSABP C-07 and C-08 phase III trials. The COLOXIS model dichotomizes patients into COLOXIS+ (oxaliplatin responder) and COLOXIS– (nonresponder) groups. Eight-year recurrence-free survival was used to evaluate oxaliplatin benefits within each of the groups, and the predictive value of the COLOXIS model was assessed using the P value associated with the interaction term (int P) between the model prediction and the treatment effect. RESULTS Among 1,065 patients, 526 were predicted as COLOXIS+ and 539 as COLOXIS–. The COLOXIS+ prediction was associated with prognosis for FULV-treated patients (hazard ratio [HR], 1.52 [95% CI, 1.07 to 2.15]; P = .017). The model was predictive of oxaliplatin benefits: COLOXIS+ patients benefited from oxaliplatin (HR, 0.65 [95% CI, 0.48 to 0.89]; P = .0065; int P = .03), but COLOXIS– patients did not (COLOXIS– HR, 1.08 [95% CI, 0.77 to 1.52]; P = .65). CONCLUSION The COLOXIS model is predictive of oxaliplatin benefits in the CC adjuvant setting. The results provide evidence supporting a change in CC adjuvant therapy: reserve oxaliplatin only for COLOXIS+ patients, but further investigation is warranted.
Improved Clinical Staging System for Localized Pancreatic Cancer Using the ABC Factors: A TAPS Consortium Study
Authors: Dekker Esther N. , van Dam Jacob L. , Janssen Quisette P. , Besselink Marc G. , DeSilva Annissa , Doppenberg Deesje , van Eijck Casper H.J. , Nasar Naaz , O'Reilly Eileen M. , Paniccia Alessandro , Prakash Laura R. , Tzeng Ching-Wei D. , Verkolf Eva M.M. , Wei Alice C. , Zureikat Amer H. , Katz Matthew H.G. , Groot Koerkamp Bas ,   for the Trans-Atlantic Pancreatic Surgery (TAPS) Consortium, Asmar Rudy el , Haviland Dana , Ilagan Crisanta , McIntyre Caitlin , McIntyre Sarah , Stoop Thomas F. , Theijse Rutger T. 
Published: February 2024

PURPOSE Previous studies suggest that besides anatomy (A: resectable, borderline resectable [BR], or locally advanced [LA]) also biologic (B: carbohydrate antigen 19-9 [CA 19-9]) and conditional (C: performance status) factors should be considered when staging patients with localized pancreatic ductal adenocarcinoma (PDAC). The prognostic value of the combined ABC factors has not been quantitatively validated. METHODS In this retrospective cohort study, we evaluated patients with localized PDAC treated with initial (modified) fluorouracil with leucovorin, irinotecan, and oxaliplatin ([m]FOLFIRINOX) at five high-volume pancreatic cancer centers in the United States and the Netherlands (2012-2019). Multivariable Cox proportional hazards analysis was used to investigate the impact of the ABC factors for overall survival (OS). RESULTS Overall, 1,835 patients with localized PDAC were included. Tumor stage at diagnosis was potentially resectable in 346 (18.9%), BR in 531 (28.9%), and LA in 958 (52.2%) patients. The baseline CA 19-9 was >500 U/mL in 559 patients (32.5%). Performance status was ≥1 in 1,110 patients (60.7%). Independent poor prognostic factors for OS were BR disease (hazard ratio [HR], 1.26 [95% CI, 1.06 to 1.50]), LA disease (HR, 1.71 [95% CI, 1.45 to 2.02]), CA 19-9 >500 U/mL (HR, 1.36 [95% CI, 1.21 to 1.52]), and WHO performance status ≥1 (HR, 1.31 [95% CI, 1.16 to 1.47]). Patients were assigned 1 point for each poor ABC factor and 2 points for LA disease. The median OS for patients with score 0-4 was 49.7, 29.9, 22.0, 19.1, and 14.9 months with corresponding 5-year OS rates of 47.0%, 28.9%, 19.2%, 9.3%, and 4.8%, respectively. CONCLUSION The ABC factors of tumor anatomy, CA 19-9, and performance status at diagnosis were independent prognostic factors for OS in patients with localized PDAC treated with initial (m)FOLFIRINOX. Staging of patients with localized PDAC at diagnosis should be based on anatomy, CA 19-9, and performance status.
Recommendations for Method Development and Validation of qPCR and dPCR Assays in Support of Cell and Gene Therapy Drug Development
Authors: Hays Amanda , Wissel Mark , Colletti Kelly , Soon Russell , Azadeh Mitra , Smith Justin , Doddareddy Rajitha , Chalfant Melanie , Adamowicz Wendy , Ramaswamy Swarna Suba , Dholakiya Sanjay L. , Guelman Sebastian , Gullick Bryan , Durham Jennifer , Rennier Keith , Nagilla Pruthvi , Muruganandham Anamica , Diaz Manisha , Tierney Cassandra , John Kaarthik , Valentine Jenny , Lockman Timothy , Liu Hsing-Yin , Moritz Benjamin , Ouedraogo Jean Paul , Piche Marie-Soleil , Smet Muriel , Murphy Jacqueline , Koenig Kaylyn , Zybura Agnes , Vyhlidal Carrie , Mercier Jonathan , Jani Niketa , Kubista Mikael , Birch Donald , Morse Karlin , Johansson Oskar 
Published: February 2024

Abstract The emerging use of qPCR and dPCR in regulated bioanalysis and absence of regulatory guidance on assay validations for these platforms has resulted in discussions on lack of harmonization on assay design and appropriate acceptance criteria for these assays. Both qPCR and dPCR are extensively used to answer bioanalytical questions for novel modalities such as cell and gene therapies. Following cross-industry conversations on the lack of information and guidelines for these assays, an American Association of Pharmaceutical Scientists working group was formed to address these gaps by bringing together 37 industry experts from 24 organizations to discuss best practices to gain a better understanding in the industry and facilitate filings to health authorities. Herein, this team provides considerations on assay design, development, and validation testing for PCR assays that are used in cell and gene therapies including (1) biodistribution; (2) transgene expression; (3) viral shedding; (4) and persistence or cellular kinetics of cell therapies.
Graphical Abstract
Preclinical Rodent Models for Human Bone Disease, Including a Focus on Cortical Bone
Authors: Koh Natalie Y Y , Miszkiewicz Justyna J , Fac Mary Louise , Wee Natalie K Y , Sims Natalie A 
Published: February 2024

Abstract
Preclinical models (typically ovariectomized rats and genetically altered mice) have underpinned much of what we know about skeletal biology. They have been pivotal for developing therapies for osteoporosis and monogenic skeletal conditions, including osteogenesis imperfecta, achondroplasia, hypophosphatasia, and craniodysplasias. Further therapeutic advances, particularly to improve cortical strength, require improved understanding and more rigorous use and reporting.
We describe here how trabecular and cortical bone structure develop, are maintained, and degenerate with aging in mice, rats, and humans, and how cortical bone structure is changed in some preclinical models of endocrine conditions (eg, postmenopausal osteoporosis, chronic kidney disease, hyperparathyroidism, diabetes). We provide examples of preclinical models used to identify and test current therapies for osteoporosis, and discuss common concerns raised when comparing rodent preclinical models to the human skeleton. We focus especially on cortical bone, because it differs between small and larger mammals in its organizational structure. We discuss mechanisms common to mouse and human controlling cortical bone strength and structure, including recent examples revealing genetic contributors to cortical porosity and osteocyte network configurations during growth, maturity, and aging. We conclude with guidelines for clear reporting on mouse models with a goal for better consistency in the use and interpretation of these models.
Alzheimer's Disease - A Comprehensive Review
Authors: Woźniak Kinga , Gardian-Baj Monika , Jung Magdalena , Hedesz Patryk , Jung Maximilian , Żuk-Łapan Aleksandra , Doryń Aleksandra , Jędral Krystian , Włodarczyk Aleksandra , Szczerbiak Alicja , Popczyńska Justyna 
Published: February 2024

Introduction: Alzheimer's disease (AD) remains a pervasive and challenging neurodegenerative disorder with profound implications for individuals, families, and societies. As the most prevalent cause of dementia globally, AD's prevalence is set to rise significantly in the context of an aging population. This introductory section emphasizes the urgent need for a comprehensive understanding of the disease, encompassing both its molecular intricacies and the socio-economic burden. With age being the primary risk factor, recent research has delved into the complex interplay of genetic predisposition and environmental influences, reshaping our understanding of AD from a multifactorial perspective.
Current State of Knowledge: Advancements in Alzheimer's research have led to a nuanced understanding of its pathology. Traditionally characterized by amyloid-beta plaques and tau tangles, recent insights from neuroimaging and biomarker studies have revealed the intricate nature of these pathologies and their roles in disease progression. Genetic studies, including genome-wide association analyses, have identified susceptibility loci, notably the APOE gene. Simultaneously, environmental factors, such as air pollution and lifestyle choices, are recognized contributors to AD risk. The therapeutic landscape has evolved, with ongoing investigations into anti-amyloid and anti-tau therapies, lifestyle interventions, and precision medicine approaches. The synthesis of these diverse findings highlights the dynamic nature of Alzheimer's research and its potential to shape future treatment modalities.
Summary: In summary, this article provides a comprehensive overview of the current state of knowledge regarding Alzheimer's disease. From understanding its evolving pathology to exploring the interplay of genetic and environmental factors, the multifactorial nature of AD is underscored. The therapeutic landscape is actively advancing, presenting a hopeful outlook for future treatments.
Intermittent fasting and its influence on health and sports results
Authors: Kowalczuk Maria , Lipińska Justyna , Lipiński Łukasz , Kopeć Izabela 
Published: February 2024

Introduction
Intermittent fasting (IF) is most simply defined as periods of eating alternating with periods of not eating. It has become one of the most popular weight-loss diets in recent years. One reason for the rapid growth in popularity of intermittent fasting may be its simplicity, as this dietary regimen does not require a change in a person's current eating patterns, nor a change in foods.
Aim of the study
This review aims to present the current state of knowledge about intermittent fasting and answer the question of what effect this form of diet has on the human body and sports results and its benefits to health.
Materials and methods
The paper was created based on the Pubmed database. The literature was reviewed using the keywords: ”intermittent fasting”, ”16/8 fasting” and ”diet standards”.
The current state of knowledge
It has been shown that intermittent fasting can be helpful in controlling body weight and reducing metabolic diseases as a result of better glucose regulation and reduced inflammation. Weight reduction can also result in improved athletic performance. In addition, IF has positive effects on the cardiovascular system, specifically improving blood pressure, lowering heart rate, and regulating the body's lipid metabolism indicators.
Summary
Although there is evidence of the beneficial effects of intermittent fasting, more research needs to be conducted to evaluate the mechanisms, efficacy in humans, target populations, and safety of intermittent fasting. Undoubtedly, a certain group of people may benefit greatly from this type of diet, as it does not require a change in current eating habits due to the simplicity of this diet.
Sleep Microbiome: Insights into Probiotics and Sleep Health
Authors: Martyka Anna , Kubicka-Figiel Martyna , Taborska Nina , Ujma Przemysław 
Published: February 2024

Introduction
The World Health Organization (WHO) defines probiotics as "live microorganisms that, when administered in adequate amounts, confer health benefits to the host." A number of scientific reports show that their effects extend beyond the immune and gastrointestinal systems, and that poor sleep quality and insomnia are an increasingly important social problem. Studies indicate that 30-50% of adults experience sleep problems. Sleep deficiencies are associated with higher rates of obesity, hypertension, type 2 diabetes, cardiovascular diseases, mental health disorders and increased risk of death. One promising way to help treat sleep problems is through the use of probiotics. Interaction through the gut-brain axis suggests that altering the gut microbiota may become a useful tool for improving sleep quality.
Aim of the study
The purpose of this study was to investigate the potential effectiveness of probiotics in improving sleep quality and treating insomnia.
Materials and Methodology
PubMed and Google Scholar databases were analyzed. Articles were searched in English using the following keywords: probiotics; sleep quality.
Results
Probiotics resulted in a favorable cumulative effect on sleep quality as measured by the PSQI global questionnaire and a statistically significant positive effect on at least one of the components characterizing sleep quality (i.e., sleep latency, sleep length, sleep disturbance, daytime sleepiness). They allow to maintain a high percentage of slow-wave sleep, despite stressors, and indirectly affect sleep quality in athletes by reducing muscle soreness.
Conclusion
The results of studies to date indicate that probiotics are an effective, safe and clinically sound adjunctive method for improving sleep quality and treating insomnia. However, these studies are at an early stage and require further efforts using larger research groups.