bluuebunny/crossref_metadata_2025_split huggingface dataset:
LMP1 and EBNA2 constitute a minimal set of EBV genes for transformation of human B cells
Authors: Zhang Jingwei , Sommermann Thomas , Li Xun , Gieselmann Lutz , de la Rosa Kathrin , Stecklum Maria , Klein Florian , Kocks Christine , Rajewsky Klaus 
Published: December 2023

IntroductionEpstein-Barr virus (EBV) infection in humans is associated with a wide range of diseases including malignancies of different origins, most prominently B cells. Several EBV latent genes are thought to act together in B cell immortalization, but a minimal set of EBV genes sufficient for transformation remains to be identified.MethodsHere, we addressed this question by transducing human peripheral B cells from EBV-negative donors with retrovirus expressing the latent EBV genes encoding Latent Membrane Protein (LMP) 1 and 2A and Epstein-Barr Nuclear Antigen (EBNA) 2.ResultsLMP1 together with EBNA2, but not LMP1 alone or in combination with LMP2A was able to transform human primary B cells. LMP1/EBNA2-immortalized cell lines shared surface markers with EBV-transformed lymphoblastoid cell lines (LCLs). They showed sustained growth for more than 60 days, albeit at a lower growth rate than EBV-transformed LCLs. LMP1/EBNA2-immortalized cell lines generated tumors when transplanted subcutaneously into severely immunodeficient NOG mice. ConclusionOur results identify a minimal set of EBV proteins sufficient for B cell transformation.
Protocol of the HISTOTHERM study: assessing the response to hyperthermia and hypofractionated radiotherapy in recurrent breast cancer
Authors: Thomsen Andreas R. , Sahlmann Jörg , Bronsert Peter , Schilling Oliver , Poensgen Felicia , May Annette M. , Timme-Bronsert Sylvia , Grosu Anca-Ligia , Vaupel Peter , Gebbers Jan-Olaf , Multhoff Gabriele , Lüchtenborg Anne-Marie 
Published: December 2023

IntroductionBreast cancer is globally the leading cancer in women, and despite the high 5-year survival rate the most frequent cause of cancer related deaths. Surgery, systemic therapy and radiotherapy are the three pillars of curative breast cancer treatment. However, locoregional recurrences frequently occur after initial treatment and are often challenging to treat, amongst others due to high doses of previous radiotherapy treatments. Radiotherapy can be combined with local hyperthermia to sensitize tumor cells to radiation and thereby significantly reduce the required radiation dose. Therefore, the combination treatment of mild local hyperthermia, i.e. locally heating of the tissue to 39-43°C, and re-irradiation with a reduced total dose is a relevant treatment option for previously irradiated patients. The mechanisms of this effect in the course of the therapy are to date not well understood and will be investigated in the HISTOTHERM study.Methods and analysesPatients with local or (loco)regional recurrent breast cancer with macroscopic tumors are included in the study. Local tumor control is evaluated clinically and histologically during the course of a combination treatment of 60 minutes mild superficial hyperthermia (39 - 43°C) using water-filtered infrared A (wIRA) irradiation, immediately followed by hypofractionated re-irradiation with a total dose of 20-24 Gy, administered in weekly doses of 4 Gy. Tumor and tumor stroma biopsies as well as blood samples will be collected prior to treatment, during therapy (at a dose of 12 Gy) and in the follow-up to monitor therapy response. The treatment represents the standard operating procedure for hyperthermia plus re-irradiation. Various tissue and blood-based markers are analyzed. We aim at pinpointing key mechanisms and markers for therapy response which may help guiding treatment decisions in future. In addition, quality of life in the course of treatment will be assessed and survival data will be evaluated.RegistrationThe study is registered at the German Clinical Trials Register, Deutsches Register Klinischer Studien (DRKS00029221).
Efficacy of radiation plus transarterial chemoembolization and lenvatinib in hepatocellular carcinoma with portal vein tumor thrombus
Authors: Dong Aoran , Zhu Meiyan , Zhang Zeyu , Fan Wenzhe , Wu Zhiqiang , Chen Yong , Tu Jianfei , Zhang Yaojun , Zhuang Wenquan , He Xiaofang , Peng Zhenwei 
Published: December 2023

BackgroundWe aimed to investigate the efficacy of a novel regimen, external beam radiation (RT) combined with trans arterial chemoembolization (TACE) and lenvatinib (LEN), in the treatment of hepatocellular carcinoma (HCC) with portal vein tumor thrombus.MethodsWe prospectively observed 102 participants from three tertiary medical centers in China between October 2018 and October 2020, who chose either RT plus TACE and LEN (RT-TACE-LEN) or TACE and LEN (TACE-LEN). LEN (12 mg or 8 mg daily) was administrated orally and continued until progression or intolerable side effects were noted. TACE was given one day after administration of LEN, and RT began within 4 weeks after the first TACE. The median dose/fraction of RT was 50 Gy/25 fractions (range: 45-60 Gy/25 fractions). Overall survival and progression free survival were compared between two groups, and complications were assessed.ResultsBoth 51 patients received RT-TACE-LEN and TACE-LEN, respectively. Most patients had tumor size&gt; 5 cm (73.8%) and tumor number≥ 2 (69.9%). The overall incidence of toxicities was significantly higher in RT-TACE-LEN group than TACE-LEN group (100% vs. 64.7%, p&lt; 0.001), but incidences of grade 3-4 toxicities were comparable (54.9% vs. 49.0%, p= 0.552). Both median overall survival (22.8 vs. 17.1 months, p= 0.031) and median progression-free survival (12.8 vs. 10.5 months, p= 0.035) were significantly longer after RT-TACE-LEN treatment than TACE-LEN.ConclusionsThe addition of RT to TACE and LEN was safe, and might improve clinical outcomes of patients with advanced HCC, which needs conformation from further studies.
Optimizing CFTR modulator therapy management for cystic fibrosis through the ReX platform
Authors: Yaacoby-Bianu Karin , Cohen-Cymberknoh Malena , Shoseyov David , Lavi Tal , Ostrovski Ana , Shteinberg Michal , Livnat Galit 
Published: December 2023

BackgroundCystic fibrosis (CF) is a chronic multi-systemic disease that requires a complex daily treatment regimen. Therefore, there is sub-optimal adherence to CF therapies, and it was shown to impact its clinical and economic burden. Cystic fibrosis transmembrane conductance regulator modulators (CFTRm) are high-cost medications that demonstrated significant benefit in clinical trials. The aim of this study was to evaluate the safety, usability, and efficacy of the ReX platform in medication management of CFTRm for the treatment of people with CF (pwCF).MethodsReX is a patient engagement platform consisting of a cloud-based management system and a cell-enabled handheld device intended to dispense oral medication into the patient's mouth, following a pre-programmed treatment protocol. It provides real-time adherence data to caregivers and timely, personalized reminders to patients. This is a prospective multi-center open study for pwCFs older than 12 years, who had been prescribed CFTRm [elexacaftor/tezacaftor/ivacaftor (ETI) or tezacaftor/ivacaftor (TI)], and provided consent to use ReX platform to receive CFTRm and record their health condition. Study duration was 12–24 months, with clinic visits where physical examination, body mass index (BMI), and pulmonary function tests were performed, and user experience questionnaires were filled in.ResultsTen pwCFs from two CF centers in Israel were included. The mean age was 31.5 years (range 15–74 years); eight were taking ETI and two TI. Median adherence to CFTRm was 97.5% (range 70%–100%) in the first year and 94% (range 84%–99%) in the second year, which is higher than the previously reported CFTRm adherence of ∼80%. No adverse events related to the use of the platform were reported. Patients reported ReX to be valuable to their treatment management and user friendly. Estimated mean forced expiratory volume in 1 s (FEV1%) increased from 74.4% to 80.8% (p = 0.004) over 2 years. Similarly, estimated BMI percentile increased from 53.5 to 59.0 (p &lt; 0.001).ConclusionsUsing the ReX platform in medication management of pwCF treated by CFTRm is safe, easy to use, and effective in improving the adherence to treatment and the clinical outcomes. Consequently, this device may potentially reduce costs to healthcare providers. Further larger and long-term studies are required to examine the clinical benefits of the ReX platform.
High-frame rate and high-count rate hybrid pixel detector for 4D STEM applications
Authors: Zambon P. , Vávra J. , Montemurro G. , Bottinelli S. , Dudina A. , Schnyder R. , Hörmann C. , Meffert M. , Schulze-Briese C. , Stroppa D. , Lehmann N. , Piazza L. 
Published: December 2023

This study presents the performance assessment of a novel hybrid pixel detector. The electron counting application-specific integrated circuit (ASIC), named KITE, was optimized for 4D STEM applications and bump-bonded to a silicon sensor that is suitable for electron energies in the range 30–200 keV. The KITE ASIC was developed for high frame rates, currently up to 120 kfps, and high count rates, owing to fast signal shaping times and instant retrigger technology, which allows operation in a unique non-paralyzable counting mode. Investigated quantities include the spectral response, the identification of optimal threshold energies, an estimation of the threshold trimming accuracy, the analysis of the single-event multiplicity distributions, the count rate capabilities, and imaging performance metrics MTF and DQE. To highlight the capabilities of the detector, the best value of 10% of linearity loss was achieved at 84 Mcts/s/pix, the best value of count rate “cutoff” at nearly 150 Mcts/s/pix, and DQE (0) between 0.75 and 0.82 up to the electron energy of 160 keV.
Ayahuasca-induced personal death experiences: prevalence, characteristics, and impact on attitudes toward death, life, and the environment
Authors: David Jonathan , Bouso José Carlos , Kohek Maja , Ona Genís , Tadmor Nir , Arnon Tal , Dor-Ziderman Yair , Berkovich-Ohana Aviva 
Published: December 2023

IntroductionDespite an emerging understanding regarding the pivotal mechanistic role of subjective experiences that unfold during acute psychedelic states, very little has been done in the direction of better characterizing such experiences and determining their long-term impact. The present paper utilizes two cross-sectional studies for spotlighting – for the first time in the literature – the characteristics and outcomes of self-reported past experiences related to one’s subjective sense of death during ayahuasca ceremonies, termed here Ayahuasca-induced Personal Death (APD) experiences.MethodsStudy 1 (n = 54) reports the prevalence, demographics, intensity, and impact of APDs on attitudes toward death, explores whether APDs are related with psychopathology, and reveals their impact on environmental concerns. Study 2 is a larger study (n = 306) aiming at generalizing the basic study 1 results regarding APD experience, and in addition, examining whether APDs is associated with self-reported coping strategies and values in life.ResultsOur results indicate that APDs occur to more than half of those participating in ayahuasca ceremonies, typically manifest as strong and transformative experiences, and are associated with an increased sense of transcending death (study 1), as well as the certainty in the continuation of consciousness after death (study 2). No associations were found between having undergone APD experiences and participants’ demographics, personality type, and psychopathology. However, APDs were associated with increased self-reported environmental concern (study 1). These experiences also impact life in profound ways. APDs were found to be associated with increases in one’s self-reported ability to cope with distress-causing life problems and the sense of fulfillment in life (study 2).DiscussionThe study’s findings highlight the prevalence, safety and potency of death experiences that occur during ayahuasca ceremonies, marking them as possible mechanisms for psychedelics’ long-term salutatory effects in non-clinical populations. Thus, the present results join other efforts of tracking and characterizing the profound subjective experiences that occur during acute psychedelic states.
High intensity interval training exercise increases dopamine D2 levels and modulates brain dopamine signaling
Authors: Tyler John , Podaras Madeline , Richardson Brittany , Roeder Nicole , Hammond Nikki , Hamilton John , Blum Kenneth , Gold Mark , Baron David A. , Thanos Panayotis K. 
Published: December 2023

BackgroundPrevious research has outlined the health benefits of exercise including its therapeutic potential for substance use disorders (SUD). These data have already been utilized and it is now common to find exercise as part of SUD treatment and relapse prevention programs. However, we need to better understand different exercise regimens and determine which would be the most beneficial for SUDs. Recently, high intensity interval training (HIIT) has gained attention in comparison with aerobic and resistance exercise. Little is known regarding the neurobiological mechanisms of HIIT, including its effects on dopamine signaling and receptor levels in the brain. The present study examined the effects of chronic HIIT exercise on dopamine signaling as measured by dopamine type 1-like receptor (D1R)-like, dopamine type 2-like receptor (D2R)-like, and tyrosine hydroxylase (TH) quantification in the brains of male and female rats as measured by [3H] SCH 23390 and [3H] spiperone autoradiography, and TH-immunoreactive optical density values.MethodsRats were separated in two groups: sedentary and HIIT exercise. Exercise was on a treadmill for 30 min daily (10 3 min cycles) for six weeks with progressive speed increased up to 0.8 mph (21.5 m/min).ResultsResults showed for D2R-like binding, a significant effect across the ventral caudate putamen (V CPU) between sexes, such that mean D2R-like binding was 14% greater for males than females. In the nucleus accumbens shell (Nac Shell), the HIIT Exercise rats showed 16% greater D2R-like binding as compared to the sedentary rats. No significant effects of HIIT exercise were found across groups for brain D1R-like binding levels or TH expression.ConclusionThese results suggest that HIIT exercise can modulate dopamine signaling by way of increased D2R. These findings support the premise that HIIT exercise plays an important role in dopamine signaling and, may provide a potential mechanism for how HIIT exercise can impact the brain and behavior.
Impact of lifestyle and psychological resilience on survival among the oldest-old in China: a cohort study
Authors: Cai Jianping , Gao Yumeng , Hu Tingfa , Zhou Lv , Jiang Hongye 
Published: December 2023

IntroductionHealthy lifestyles and psychological resilience are important factors influencing the life expectancy of the oldest-old (≥80 years). Stratified by urban and rural groups, this study used a 10-year cohort to examine the mechanism of lifestyle and psychological resilience on the survival of the oldest-old in China.MethodsThis study used the China Longitudinal Healthy Longevity Survey datasets spanning from 2008 to 2018, and 9,250 eligible participants were included. The primary outcome variable was all-cause mortality, and independent variables included healthy lifestyle index and psychological resilience. Six covariates were included in the survival analysis and moderation-mediation model, such as gender and annual household income.ResultsThis study found that the oldest-old with five healthy lifestyles had the longest survival time, averaging 59.40 months for urban individuals and 50.08 months for rural individuals. As the lifestyle index increased, the survival rate significantly increased. The Cox regression showed that for the urban oldest-old, the lifestyle index served as a protective factor for survival outcomes. However, this effect lost statistical significance among rural oldest-old individuals. For urban oldest-old individuals, psychological resilience significantly mediated and moderated the effect of the lifestyle index on survival status, but the moderating effect was not statistically significant for the rural ones.DiscussionOverall, healthy lifestyles and psychological resilience can be effective in enhancing the survival of the oldest-old, and there are differences between urban and rural population, so different interventions should be adopted for urban and rural areas to achieve longer life in China.
Cross-sectional survey exploring current intake practices for dogs admitted to animal shelters in Texas: a descriptive study
Authors: Cranford Mackenzie , Bing Abbey , Cisneros Alissa , Carroll Amber D. , Porter Hannah , Stellato Anastasia Chiara 
Published: December 2023

IntroductionEntering an animal shelter is a stressful experience for dogs that can impair their welfare, adoptability, and shelter staff safety; thus, it is crucial to reduce the stress experienced during intake. This study investigated the current intake practices for dogs admitted in animal shelters in Texas, United States.MethodsTo gather data, an online survey was designed and distributed to shelter employees responsible for intake at animal shelters. The survey collected information about examination procedures, the type of information collected from owner-surrenders, as well as the housing environment for the dogs.ResultsSurvey participants (n = 64) were shelter staff from municipal (59%, 38/64) and private shelters (23%, 15/64) in 47 counties. Handling techniques reported to be used during intake exams varied depending on the dog’s behavior, with participants reporting higher restraint for aggressive dogs and lower restraint for calm dogs. If the dog was displaying fear, participants reported offering food and attention (89%, 47/53), using towel restraint (64%, 34/53) and conducting the exam on someone’s lap (49%, 26/53). In cases of aggression, it was commonly reported to use muzzles (81%, 42/52) and catch poles (77%, 40/52), and shorten the exam (71%, 37/52). After the exam, most reported placing dogs on the adoption floor (45%, 27/60) or placing them wherever space was available (20%, 12/60).DiscussionResults provide descriptive information on current intake procedures and routine handling techniques used in Texas shelters. Future research should explore shelter dog responses to routine handling techniques to support the development of evidence-based protocols during routine intake examinations and procedures.
MODEL KEPERAWATAN ADAPTASI CALISTA ROY TERHADAP BODY IMAGE PADA IBU HAMIL TRIMESTER III
Authors: Surtiati Eti , Sunita Anna , Nuraeni Ati , Susilowati Dwi 
Published: December 2023

Model keperawatan Adaptasi Callista Roy ini memandang bahwa setiap manusia pasti mempunyai potensi untuk dapat beradaptasi terhadap stimulus baik stimulus internal maupun eksternal dan kemampuan adaptasi ini dapat dilihat dari berbagai tingkatan usia. Ibu hamil dapat merasakan kekhawatirannya mengenai perubahan bentuk tubuh yang terjadi dan dapat juga merasa ragu akan kemampuan peran barunya sebagai ibu, sehingga pengalaman ini menimbulkan bermacam rasa antara bahagia dan cemas selama kehamilan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh model keperawatan Adaptasi Fungsi Fisiologis Callista Roy terhadap body image ibu hamil trimester III di Puskesmas kota Bogor. Penelitian ini menggunakan desain quasi eksperimental pre post with control group design. Pengumpulan data menggunakan instrumen berupa kuesioner untuk mengukur adaptasi psikologis. Populasi dalam penelitian ini adalah seluruh ibu hamil trimester III yang berkunjung di Puskesmas Pancasan Kota Bogor yang memenuhi kriteria inklusi dan esklusi berjumlah 40 ibu. Sampel terdiri atas 20 orang kelompok perlakuan dan 20 orang kelompok kontrol. Analisis univariat menggunakan distribusi frekuensi. Analisis bivariat menggunakan uji t-independent. Hasil penelitian menunjukan bahwa terdapat perbedaan body image ibu hamil trimester III antara kelompok kontrol dan kelompok intervensi sesudah diberikan model keperawatan adaptasi fungsi fisiologi Callista Roy dengan nilai p=0,032 untuk body image. Dapat disimpulkan bahwa ada pengaruh yang bermakna pada body image ibu hamil setelah diberikan model keperawatan adaptasi fungsi fisiologi Callista Roy.
أثر طريقة الصورة بالصورة على تنمية مهارة الكلام لدى الطلاب بالمدرسة الثانوية الثانية ستوبوندو
Authors: Yakin Wilda Fitria , Alfan Muhammad , Haq Fahim Aghna 'lzzil 
Published: December 2023

Learning is an activity carried out by educators and learners in an environment that aims to achieve a learning goal. The fact is that in schools, there are still many educators who still need to achieve learning goals, especially learning maharah kalam. Therefore, the problem in schools is the low value of students in learning maharah kalam. This is due to an educator's need for more initiative in using several components that support the achievement of learning goals. One of these components is the learning method. Thus, the researchers used the picture-and-picture Learning method to develop students' Maharah Kalam. This study aimed to determine how the picture and picture method has influenced the development of Arabic speaking skills among students in MAN 2 Situbondo. In this study, researchers used quantitative approaches and types of experiments. This study's experiments and data collection methods use tests, questionnaires, observation, and documentary. Analysis of the data using the formula of the product of moments: r = nΣxy – (Σx)(Σy) /√{nΣx² – (Σx)²} {nΣy2 – (Σy)2}. The results of this study showed that the effect of the picture-and-picture method on the development of students ' speaking skills at an error rate of 5% is powerful. The average test result in the experimental class was 86.7, and in the observation class, 69.9. The data analysis results for the t-test calculation are 8.71 and T-schedule 1.67 or 8.71>1.67.
Edukasi Di Era Covid-19 Melalui Program KKN-DR IAIN Jember Untuk Mewujudkan Desa Karangkedawung Cerdas dan Sehat
Authors: Hasyim Hafidz , Rosidah Rosidah 
Published: December 2023

Corona virus Disease-2019 (COVID-19) diketahui menyebabkan infeksi saluran nafas pada manusia mulai dari batuk pilek hingga yang lebih serius seperti Middle East Respiratory Syndrome (MERS) dan Severe Acute Respiratory Syndrome (SARS). Persebaran virus COVID-19 meningkat semakin cepat melalui tetesan air liur atau keluar dari hidung ketika orang yang terinfeksi batuk atau bersin. Akan tetapi, masih sangat minim pemahaman dan kesadaran masyarakat di Rt 01 Rw 03 Desa Karangkedawung, Kecamatan Mumbulsari Kabupaten Jember yang mematuhi protokol kesehatan saat beraktivitas di luar rumah. Lokasi tersebut merupakan kawasan yang ramai penduduk. Oleh karena itu, perlunya peningkatan pemahaman COVID-19 dan pentingnya mematuhi protokol kesehatan penanggulangan COVID-19 serta pentingnya pendidikan bagi anak-anak sekolah pada Era COVID-19.
Berdasakan permasalahan tersebut, penulis melakukan pengabdian pada masyarakat melalui kegiatan Kuliah Kerja Nyata Dari Rumah (KKN-DR) Era Covid-19 guna meminimalisir terjadinya penyebaran wabah COVID-19. Kegiatan yang dilakukan dengan metode Edukasi COVID-19 Kepada Masyarakat Rt 01 Rw 03 Desa Karangkedawung. Pada program KKN ini dilakukan sosialisasi tanggap masyarakat dengan adanya COVID-19, Pendidikan terhadap anak sekolah yang mengalami proses pembelajaran online (daring) dan serta edukasi tentang penerapan protokol kesehatan di lingkungan Rt 01 Rw 03 Desa Karangkedawung yang bertujuan dimanapun warga karangkedawung melakukan aktifitasnya supaya lebih mematuhi lagi akan pentingnya protokol kesehatan.