bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing Diversity in Undergraduate Degree Programs in Forestry and Related Natural Resources: a Brief Review of Critical Issues and Promising Actions
Authors: Bullard Steven H , Walker T J , Burger Leslie 
Published: November 2023

AbstractAlthough forestry and related natural resources (FRNR) undergraduate degree programs in the United States have become increasingly diverse in recent years, women and minorities remain underrepresented in multiple disciplines; this is particularly true in forestry undergraduate programs. We reviewed over one hundred reports and publications to help understand the critical issues that must be addressed to enhance diversity in FRNR undergraduate programs. Based on that review, here we summarize eight critical issues in recruiting and retaining a more diverse FRNR undergraduate student body, and we also present promising actions to address the issues. Our goal is to more widely communicate validated ideas and practices, thereby prompting further discussion and effective actions to make more tangible gains in enhancing the diversity of FRNR undergraduate degree programs in the United States in the future.Study Implications: To be effective managers and stewards of forestry and related natural resources (FRNR), professionals must be effective in developing, communicating, and implementing plans and actions. From recommendations for a single stand or forest, to community-level natural resource issues, to state, regional, and national policy decisions, FRNR professionals must be trusted by increasingly diverse stakeholders in our society. FRNR professions can become more diverse in terms of gender as well as race and ethnicity by developing and implementing actions that address specific critical issues in undergraduate enrollment. A more diverse and society-ready cadre of leaders in FRNR will help to ensure healthy biotic communities are restored, sustained, and valued while meeting long-run societal needs.
The CFD simulation of E1619 propeller open-water tests validated by the EFD in the NCKU towing tank
Authors: Lin Yu-Hsin , Hasan Ahmad Darori 
Published: November 2023

Abstract
This study established a Computational Fluid Dynamics (CFD) model based on a dynamic mesh strategy to conduct a comprehensive numerical investigation of the performance of the E1619 propeller in open-water tests. To capture the turbulence flows around the propeller, a large eddy simulation (LES) turbulence model was implemented. In order to validate the simulation results, a series of open-water tests were conducted in the towing tank at National Cheng Kung University, resulting in a successful achievement of total uncertainties of less than ∼6%. The approach of simultaneous grid and time refinement was utilized to perform the discretization analysis. Eventually, the simulation results were employed to analyze the hydrodynamic performance and flow structure around the propeller, resulting in a conclusion based on the attained level of accuracy. The results indicate that the cases for a propeller with a strut exhibit favorable predictions compared to those of a single propeller, with error values for the thrust coefficients and propeller efficiencies falling below 6%. On the other hand, the torque coefficient was more accurately estimated for the cases of a single propeller than for those of a propeller with a strut, with error values below 2%.
Comparing the efficacy of endoscopic endonasal versus microscopic transsphenoidal approach for the management of sellar and suprasellar tumors: a systematic review and meta-analysis protocol
Authors: Tango Tamara , Daniel Jeremiah Oluwatomi Itodo , Mugenyi Nathan , Abongha Gobti Beltus , Mesfin Samuel , Javed Saad , Omran Murad Othman , Eltingary Mazin M , Kyaruzi Victor Meza 
Published: November 2023

Abstract
Managing sellar and suprasellar tumors is challenging due to their diverse origins and proximity to critical structures. The transsphenoidal surgical approach has become the preferred method, evolving from transcranial attempts to reduce morbidity. Microscopic transsphenoidal techniques have been refined over time, with endoscopic endonasal surgery emerging as a significant advancement. Due to the multifaceted nature of these approaches, the complexities of the pathology and the paucity of data from specific regions, understanding the prevalence, characteristics and management outcomes of sellar and suprasellar tumors is crucial for informing healthcare practices and resource allocation. This study aims to collate evidence-based insights into the efficacy of the endoscopic endonasal approach compared with the microscopic transsphenoidal approach as a surgical intervention for sellar and suprasellar tumors, following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines for 2020. To source pertinent information, we will rely on four primary central databases: PubMed, EMBASE, SCOPUS and the Cochrane Central Register of Controlled Trials. The primary outcome measure will be the surgical outcome’s effectiveness in achieving tumor resection and preserving pituitary function. The study protocol is duly registered with the International Prospective Register of Systematic Reviews (PROSPERO), with no requirement for ethical approval as it exclusively relies on previously published research. We aim to publish our findings in scientific journals and present the abstract at conferences to guide clinical practice. PROSPERO Registration Number: CRD42023461972: available via https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023461972.
Advancements in robotic arm-based 3D bioprinting for biomedical applications
Authors: Li Kai , Huang WenHui , Guo HaiTao , Liu YanYan , Chen Shuxian , Liu Heng , Gu Qi 
Published: November 2023

Abstract
3D bioprinting emerges as a critical tool in biofabricating functional 3D tissue or organ equivalents for regenerative medicine. Bioprinting techniques have been making strides in integrating automation, customization, and digitalization in coping with diverse tissue engineering scenarios. The convergence of robotic arm-based 3D bioprinting techniques, especially in situ 3D bioprinting, is a versatile toolbox in the industrial field, promising for biomedical application and clinical research. In this review, we first introduce conceptualized modalities of robotic arm-based bioprinting from a mechanical perspective, which involves configurative categories of current robot arms regarding conventional bioprinting strategies. Recent advances in robotic arm-based bioprinting in tissue engineering have been summarized in distinct tissues and organs. Ultimately, we systematically discuss relative advantages, disadvantages, challenges, and future perspectives from bench to bedside for biomedical application.
Better Existing Water, Sanitation, and Hygiene Can Reduce the Risk of Cholera in an Endemic Setting: Results From a Prospective Cohort Study From Kolkata, India
Authors: Islam Md Taufiqul , Im Justin , Ahmmed Faisal , Kim Deok Ryun , Tadesse Birkneh Tilahun , Kang Sophie , Khanam Farhana , Chowdhury Fahima , Ahmed Tasnuva , Firoj Md Golam , Aziz Asma Binte , Hoque Masuma , Park Juyeon , Jeon Hyon Jin , Kanungo Suman , Dutta Shanta , Zaman Khalequ , Khan Ashraful Islam , Marks Florian , Kim Jerome H , Qadri Firdausi , Clemens John D 
Published: November 2023

Abstract

Background
Global cholera control efforts rely heavily on effective water, sanitation, and hygiene (WASH) interventions in cholera-endemic settings.

Methods
Using data from a large, randomized controlled trial of oral cholera vaccine conducted in Kolkata, India, we evaluated whether natural variations in WASH in an urban slum setting were predictive of cholera risk. From the control population (n = 55 086), baseline WASH data from a randomly selected “training subpopulation” (n = 27 634) were analyzed with recursive partitioning to develop a dichotomous (“better” vs “not better”) composite household WASH variable from several WASH features collected at baseline, and this composite variable was then evaluated in a mutually exclusive “validation population” (n = 27 452). We then evaluated whether residents of better WASH households in the entire population (n = 55 086) experienced lower cholera risk using Cox regression models. Better WASH was defined by a combination of 4 dichotomized WASH characteristics including safe source of water for daily use, safe source of drinking water, private or shared flush toilet use, and always handwashing with soap after defecation.

Results
Residence in better WASH households was associated with a 30% reduction in risk of cholera over a 5-year period (adjusted hazard ratio, 0.70 [95% confidence interval, .49–.99]; P = .048). We also found that the impact of better WASH households on reducing cholera risk was greatest in young children (0–4 years) and this effect progressively declined with age.

Conclusions
The evidence suggests that modest improvements in WASH facilities and behaviors significantly modify cholera risk and may be an important component of cholera prevention and elimination strategies in endemic settings.
Clinical Trials Registration. NCT00289224.
The 14-3-3 protein GRF8 modulates salt stress tolerance in apple via the WRKY18-SOS pathway
Authors: Fan Zihao , Zhu Yuqing , Kuang Wei , Leng Jun , Wang Xue , Qiu Linlin , Nie Jiyun , Yuan Yongbing , Zhang Rui-Fen , Wang Yongzhang , Zhao Qiang 
Published: November 2023

Abstract
Salinity is a severe abiotic stress that limits plant survival, growth, and development. 14-3-3 proteins are phosphopeptide-binding proteins that are involved in numerous signaling pathways, such as metabolism, development, and stress responses. However, their roles in salt tolerance are unclear in woody plants. Here, we characterized an apple (Malus domestica) 14-3-3 gene, GENERAL REGULATORY FACTOR 8 (MdGRF8), the product of which promotes salinity tolerance. MdGRF8 overexpression improved salt tolerance in apple plants, whereas MdGRF8-RNA interference (RNAi) weakened it. Yeast 2-hybrid, bimolecular fluorescence complementation, pull-down, and coimmunoprecipitation assays revealed that MdGRF8 interacts with the transcription factor MdWRKY18. As with MdGRF8, overexpressing MdWRKY18 enhanced salt tolerance in apple plants, whereas silencing MdWRKY18 had the opposite effect. We also determined that MdWRKY18 binds to the promoters of the salt-related genes SALT OVERLY SENSITIVE 2 (MdSOS2) and MdSOS3. Moreover, we showed that the 14-3-3 protein MdGRF8 binds to the phosphorylated form of MdWRKY18, enhancing its stability and transcriptional activation activity. Our findings reveal a regulatory mechanism by the MdGRF8–MdWRKY18 module for promoting the salinity stress response in apple.
Near-term investments in forest management support long-term carbon sequestration capacity in forests of the United States
Authors: Coulston John W , Domke Grant M , Walker David M , Brooks Evan B , O’Dea Claire B 
Published: November 2023

Abstract
The forest carbon sink of the United States offsets emissions in other sectors. Recently passed US laws include important climate legislation for wildfire reduction, forest restoration, and forest planting. In this study, we examine how wildfire reduction strategies and planting might alter the forest carbon sink. Our results suggest that wildfire reduction strategies reduce carbon sequestration potential in the near term but provide a longer term benefit. Planting initiatives increase carbon sequestration but at levels that do not offset lost sequestration from wildfire reduction strategies. We conclude that recent legislation may increase near-term carbon emissions due to fuel treatments and reduced wildfire frequency and intensity, and expand long-term US carbon sink strength.
Tissue adhesive, ROS scavenging and injectable PRP-based ‘plasticine’ for promoting cartilage repair
Authors: Li Shiao , Niu Dawei , Fang Haowei , Chen Yancheng , Li Jinyan , Zhang Kunxi , Yin Jingbo , Fu Peiliang 
Published: November 2023

Abstract
Platelet-rich plasma (PRP) that has various growth factors has been used clinically in cartilage repair. However, the short residence time and release time at the injury site limit its therapeutic effect. The present study fabricated a granular hydrogel that was assembled from gelatin microspheres and tannic acid through their abundant hydrogen bonding. Gelatin microspheres with the gelatin concentration of 10 wt% and the diameter distribution of 1–10 μm were used to assemble by tannic acid to form the granular hydrogel, which exhibited elasticity under low shear strain, but flowability under higher shear strain. The viscosity decreased with the increase in shear rate. Meanwhile, the granular hydrogel exhibited self-healing feature during rheology test. Thus, granular hydrogel carrying PRP not only exhibited well-performed injectability but also performed like a ‘plasticine’ that possessed good plasticity. The granular hydrogel showed tissue adhesion ability and reactive oxygen species scavenging ability. Granular hydrogel carrying PRP transplanted to full-thickness articular cartilage defects could integrate well with native cartilage, resulting in newly formed cartilage articular fully filled in defects and well-integrated with the native cartilage and subchondral bone. The unique features of the present granular hydrogel, including injectability, plasticity, porous structure, tissue adhesion and reactive oxygen species scavenging provided an ideal PRP carrier toward cartilage tissue engineering.
Administered dosage and effective dose estimated from 81Rb-rubidium hydroxide for lung ventilation scintigraphy using 81mKr noble gas
Authors: Kamiya Takashi , Iimori Takashi , Maeda Yukito , Yada Nobuhiro , Hayashi Naoya , Iguchi Harumi , Narita Masataka 
Published: November 2023

Abstract
The aim of this study was to estimate the administered dosage of 81mKr noble gas as calculated by the radioactivity of 81Rb-rubidium hydroxide (81RbOH). The administered dosage was regarded as the total amount of 81mKr noble gas. The radioactivity of 81mKr was calculated using the radioactivity of 81RbOH at the examination, the beginning of inhalation, the inhalation duration and the attenuation volume from the generator to the patient for 81mKr noble gas. In addition, we created an Internet survey and asked National University Hospital in Japan to respond to questions regarding the parameters of concern. Survey responses were provided by 38 hospitals (response rate was 90.5%). Twenty-seven hospitals (64.3%) examined lung ventilation scintigraphy using 81mKr noble gas. The mean administered dosage and the effective dose of lung ventilation scintigraphy using 81mKr noble gas were 35.8 ± 22.1 GBq and 0.97 ± 0.60 mSv, respectively.
On the coupling and decoupling of mind wandering and perception: a shared metabolism account
Authors: Bruckmaier M , Albrecht V , Tachtsidis I , Lavie N 
Published: November 2023

Abstract

Introduction
Mind wandering (MW) has been associated with reduced responsiveness to external stimuli (“perceptual decoupling”). Conversely, increased perceptual demands of a task result in reduced MW. Here we propose a neurobiological account attributing the mutually-limiting relationship of MW and perception to brain-wide limits on cerebral metabolism. Since overall cerebral metabolism is known to remain constant, despite increased mental task demands, we tested whether increased perceptual processing load in a visual task will result in reduced oxygen metabolism in MW-related medial prefrontal cortex (mPFC) regions.

Methods
We used broadband near-infrared spectroscopy to measure oxidation states of the cytochrome-c-oxidase enzyme (oxCCO), an intracellular marker of metabolism, in mPFC while sampling participants’ MW experiences during their performance of a visual task of either low (feature search) or high(conjunction search) perceptual load.

Results
Increased perceptual load in the task resulted in reduced oxCCO signal in mPFC regions related to MW reports. High perceptual load was also found to specifically suppress detailed (and hence more metabolism-demanding) rather than vague MW.

Discussion
Overall, the results support a shared metabolism account of the relationship between MW and perception and demonstrate that attentional-regulation of metabolism only supports ongoing detailed MW when perceptual processing demands are low.
High Diversity of Curtobacterium flaccumfaciens in Poinsettia and Detection of Three Pathogenicity Plasmids for Identification of C. flaccumfaciens pv. poinsettiae
Authors: Bultreys Alain , Gheysen Isabelle 
Published: November 2023

Outbreaks in Europe have raised concerns about poinsettia bacterial canker caused by Curtobacterium flaccumfaciens pv. poinsettiae, described in the United States. Using a semiselective medium containing aztreonam and fosfomycin and selection during isolation based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, 424 Curtobacterium strains were isolated from Belgian poinsettias of African and European origin. Different populations coexisted: 130 strains were identified as C. flaccumfaciens with scores ≥2.0 and 294 with scores <2.0 or as another Curtobacterium. MALDI-TOF MS libraries constructed using similar medium and extraction procedure and a pathogenicity test on poinsettia were used to screen collections from poinsettia and wheat, a possible alternative host, for C. flaccumfaciens pv. poinsettiae. The concatenated recA- gyrB partial sequences showed that 114 poinsettia or wheat strains belonged to different Curtobacterium species. Eighty-eight nonpathogenic strains and four U.S. strains from litter were intermingled with bean pathogenic C. flaccumfaciens pv. flaccumfaciens strains in the three genetic groups of C. flaccumfaciens but lacked their pathogenicity markers. Four new European recA- gyrB sequatypes of C. flaccumfaciens pv. poinsettiae, obtained from symptomatic and asymptomatic poinsettias, were distinguished from three American sequatypes. Six sequatypes were pathogenic in tests, belonged to genetic groups related to two different genomospecies, and possessed a plasmid. Sequencing of six plasmids revealed three related plasmids containing proteases and a polygalacturonase found only in strains pathogenic in pathogenicity tests and specifically identified by polygalacturonase-based PCR and loop-mediated isothermal amplification assays. Similarities between plant and litter Curtobacterium and the role of plasmids in pathogenicity and punctual transmission of pathogenicity among heterogeneous C. flaccumfaciens are suggested.
Chromosome-Scale Genome Sequence Resource for Two Neopestalotiopsis spp. Isolates with Different Virulence in Strawberry (Fragaria × ananassa)
Authors: Han Hyeondae , Jang Yoon Jeong , Oh Youngjae , Marin Marcus Vinicius , Huguet-Tapia Jose , Peres Natalia A. , Lee Seonghee 
Published: November 2023

Several Neopestalotiopsis spp. have been reported to affect strawberry worldwide. Here, we sequenced two isolates of Neopestalotiopsis 19-02 (highly virulent) and 13-481 (moderately virulent) recovered from commercial strawberry fields in Florida and generated chromosome-scale assemblies using the Oxford Nanopore Technologies and Illumina platforms. Each genome assembly for 19-02 and 13-481 contained putative telomere sequences at the 5′ and 3′ ends. By analyzing the collinearity of the two newly assembled genomes and verifying the conserved telomere sequence at the end of each chromosome, we confirmed that the genome of Neopestalotiopsis spp. consists of seven basic chromosomes. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis showed 98.5% of conserved core genes for both genome assemblies, 19-02 and 13-481. Phylogenetic analysis with internal transcribed spacer, beta-tubulin, and translation elongation factor regions obtained from the assemblies 19-02 and 13-481 demonstrated that the highly virulent isolate 19-02 is phylogenetically distinct from moderate virulent N. rosae 13-481. Comparative genome analysis of the high-quality reference genome assemblies provided in this study will facilitate the identification of genomic regions responsible for the different virulence of the Neopestalotiopsis pathogen to strawberry. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.
Genomic Resource Insights into Strain WZ-12: An Antimicrobial-Producing Streptomyces Isolate from Star Anise Rhizosphere Soil
Authors: Pan Jieming , Geng Xiaoshan , Zhu Yulin , Liu Qin 
Published: November 2023

Strain WZ-12, isolated from the rhizosphere soil of star anise trees, exhibits significant antifungal efficacy against a variety of fungi, including Colletotrichum horii, the pathogenic fungus responsible for anthracnose in star anise. Based on 16S rRNA gene sequencing and phylogenetic analysis, the strain was identified as a member of the Streptomyces genus. The genome of WZ-12 comprises a linear chromosome of 9,169,400 bp and a plasmid of 378,325 bp, possessing a G + C content of 71.12%. Of note, the genome encompasses 8,172 protein-coding genes, 21 rRNA genes, 74 tRNA genes, and 89 other noncoding RNA genes. The COG, Swiss-Prot, GO, KEGG, and NR databases annotated up to 79.17, 50.50, 13.17, 37.51, and 90.43% of potential genes, respectively. Remarkably, the genome hosts 175 antimicrobial resistance genes and 33 secondary metabolite gene clusters that likely contribute to the biosynthesis of diverse bioactive compounds such as antibiotics and agents with antitumor, antioxidant, immunosuppressive, anti-inflammatory, or stress tolerance properties. These findings highlight the potential of WZ-12 as a promising source of novel bioactive molecules and suggest its significant value as a resource for developing innovative antimicrobial agents. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .