bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the cell membrane localization of PADI4 in breast cancer cells and inhibition of anti-PADI4 monoclonal antibody
Authors: Wang Yan , Song Xianqin , Song Yu , Fang Kehua , Chang Xiaotian 
Published: October 2023

Abstract
Background
Peptidyl arginine deiminase 4 (PADI4) is a post-translational modification enzymecan that converts arginine in protein into citrulline in the presence of calcium ions, which is called citrullination. PADI4 has been reported to be expressed in the cytoplasm and nucleus in a variety of malignant tumors. Based on the GeneCards database and our previous research, it is speculated that PADI4 may also be expressed on the cell membrane. This study aimed to confirm the membrane expression of PADI4 and the effect of anti-PADI4 antibodies on cell membrane PADI4. This may be another mechanism of action of anti-PADI4 monoclonal antibodies in the treatment of breast cancer.

Methods
The subcellular localizations of PADI4 in MDA-MB-231 and MCF-7 breast cancer cells were determined by immunofluorescence, immunoelectron microscopy, and Western blot analysis. The tumor cells were treated with PADI4 antibody, and cell proliferation, migration, colony formation, apoptosis, glycolysis, and epithelial-mesenchymal transition (EMT) were measured as well as the expression of some essential tumor genes.

Results
PADI4 was not only localized in the nucleus and cytoplasm of breast cancer cells but was also detected on the cell membrane. Following PADI4 antibody treatment, cell proliferation, migration, colony formation, EMT, and ATP production through glycolysis were decreased, and the mRNA expression of MYC proto-oncogene (MYC), FAT atypical cadherin 1 (FAT1), nuclear factor kappa B subunit 1 (NFκB), and tumor necrosis factor (TNF-α) in breast cancer cells was downregulated, while the mRNA expression of tumor protein p63 (TP63) was upregulated.

Conclusions
PADI4 is expressed on the cell membrane in breast cancer cells. Anti-PADI4 antibodies can affect the biological functions of cell membrane PADI4, including proliferation, migration, apoptosis, and glycolysis, thereby inhibiting tumor progression.

Graphical abstract
Periodic Steiner Networks Minimizing Length
Authors: Alex Jerome , Grosse-Brauckmann Karsten 
Published: October 2023

AbstractWe study a problem of geometric graph theory: We determine the triply periodic graph in Euclidean 3-space which minimizes length among all graphs spanning a fundamental domain with the same volume. The minimizer is the so-called network with quotient the complete graph on four vertices $$K_4$$

K
4

. For comparison we consider a competing topological class, also with a quotient on four vertices, and determine the minimizing networks.
Dye-free visualisation of intestinal perfusion using laser speckle contrast imaging in laparoscopic surgery: a prospective, observational multi-centre study
Authors: Heeman Wido , Calon Joost , van der Bilt Arne , Pierie Jean-Pierre E. N. , Pereboom Ilona , van Dam Gooitzen M. , Boerma E. Christiaan 
Published: October 2023

Abstract
Introduction
Intraoperative perfusion imaging may help the surgeon in creating the intestinal anastomoses in optimally perfused tissue. Laser speckle contrast imaging (LSCI) is such a perfusion visualisation technique that is characterized by dye-free, real-time and continuous imaging. Our aim is to validate the use of a novel, dye-free visualization tool to detect perfusion deficits using laparoscopic LSCI.

Methods
In this multi-centre study, a total of 64 patients were imaged using the laparoscopic laser speckle contrast imager. Post-operatively, surgeons were questioned if the additional visual feedback would have led to a change in clinical decision-making.

Results
This study suggests that the laparoscopic laser speckle contrast imager PerfusiX-Imaging is able to image colonic perfusion. All images were clear and easy to interpret for the surgeon. The device is non-disruptive of the surgical procedure with an average added surgical time of 2.5 min and no change in surgical equipment. The potential added clinical value is accentuated by the 17% of operating surgeons indicating a change in anastomosis location. Further assessment and analysis of both white light and PerfusiX perfusion images by non-involved, non-operating surgeons showed an overall agreement of 80%.

Conclusion
PerfusiX-Imaging is a suitable laparoscopic perfusion imaging system for colon surgery that can visualize perfusion in real-time with no change in surgical equipment. The additional visual feedback could help guide the surgeons in placing the anastomosis at the most optimal site.
Characteristic of bioclimatic conditions in Poland based on Physiologically Equivalent Temperature
Authors: Tomczyk Arkadiusz M. , Matzarakis Andreas 
Published: October 2023

AbstractThe aim of the study was to characterise bioclimatic conditions in Poland in the times of progressing warming. This type of research permits the verification whether the progressing climate warming also translates into a change in bioclimatic conditions. This study was based on data obtained for the period 1966–2021 from 37 synoptic stations located in Poland. All the data series were uniform and obtained from the data base of the Institute of Meteorology and Water Management—National Research Institute (IMGW-PIB). The study revealed high variability of bioclimatic conditions in Poland both in spatial and in temporal terms. The lowest mean annual PET values were recorded in the north and north-east of the country and the highest in the south-west of Poland. The study revealed changes in the frequency of occurrence of days with cold and heat stress, as well as days with no thermal stress. The most intensive changes were determined for days with cold stress. A decrease in the number of days in this category translated into an increase in the number of days with no thermal stress and days with heat stress.
Predictors for spinal deformity following resection of intramedullary tumor via posterior approach: a systematic review and meta-analysis
Authors: Szyduczyński Maciej , Korneliussen Johannes , Landé Oscar , Krakowiak Michał , Szmuda Tomasz , Miękisiak Grzegorz 
Published: October 2023

Abstract
Purpose
The present study aimed to identify the clinical predictive factors for worsened spinal deformity (SD) following surgical resection via posterior approach for primary intramedullary tumors.

Methods
A systematic search was performed using PubMed, Web of Science, and Scopus databases to extract potential references. Observational studies reporting predictive factors for worsened SD following surgical resection via posterior approach for primary intramedullary tumors were included. The odds ratio (OR) was calculated for dichotomous parameters.

Results
Four retrospective cohort studies were included in the meta-analysis. They were comprised of two groups of patients; those who developed SD (n = 87) and those who did not (n = 227). For patients with IMSCTs, age under 25 years as well as age under 13 years were the demographic variables associated with postoperative SD (odds ratio [OR] 3.92; p = 0.0002 and OR 4.22; p = 0.003). In both the fusion and the non-fusion subgroups, preoperative spinal deformity strongly predicted postoperative SD (OR 11.94; p < 0.001), with the risk highly elevated among the non-fusion patients (OR 24.64; p < 0.0002). Thoracolumbar junction involvement was also found to be a predictor of postoperative SD for patients with IMSCT (OR 2.89; p = 0.02).

Conclusion
This study highlights the importance of considering age, preoperative spinal deformity, and thoracolumbar junction involvement as predictors of postoperative spinal deformity following surgical resection for IMSCT. These findings may provide guidance for the management of these patients, including the development of preoperative planning strategies and the selection of the most appropriate surgical approach for high-risk patients.
Targeted muscle reinnervation in upper extremity amputations
Authors: Le Elliot L. H. , Iorio Matthew L. , Greyson Mark A. 
Published: October 2023

Abstract
Purpose
Targeted muscle reinnervation (TMR) is a relatively recent surgical innovation that involves the coaptation of major peripheral nerves to a recipient motor branch that innervates an expendable muscle target. The original indication for TMR was augmentation and optimization of myoelectric signals in the amputated limb for use of myoelectric prosthetics. Incidentally, surgeons and patients discovered that the technique also could treat and prevent phantom and residual limb pain. TMR is performed at the time of amputation or delayed any time after the amputation, and TMR can also be performed at any level of amputation. In the upper extremity, studies have detailed the various techniques and coaptations possible at each amputation level to create intuitive myoelectric signals and treat neurogenic pain. Treatment of peripheral nerves in the amputee with TMR should be a consideration for all patients with major upper extremity amputations, especially at large institutions able to support multidisciplinary limb salvage teams. This review article summarizes the current literature and authors’ techniques and recommendations surrounding TMR in the upper extremity amputee including techniques relevant to each level of upper extremity amputation.
Facial emotion expressivity in patients with Parkinson’s and Alzheimer’s disease
Authors: Cannavacciuolo Antonio , Paparella Giulia , Salzillo Martina , Colella Donato , Canevelli Marco , Costa Davide , Birreci Daniele , Angelini Luca , Guerra Andrea , Ricciardi Lucia , Bruno Giuseppe , Berardelli Alfredo , Bologna Matteo 
Published: October 2023

AbstractParkinson’s disease (PD) and Alzheimer’s disease (AD) are neurodegenerative disorders with some overlapping clinical features. Hypomimia (reduced facial expressivity) is a prominent sign of PD and it is also present in AD. However, no study has experimentally assessed hypomimia in AD and compared facial expressivity between PD and AD patients. We compared facial emotion expressivity in patients with PD, AD, and healthy controls (HCs). Twenty-four PD patients, 24 AD patients and 24 HCs were videotaped during neutral facial expressions and while posing six facial emotions (anger, surprise, disgust, fear, happiness, and sadness). Fifteen raters were asked to evaluate the videos using MDS-UPDRS-III (item 3.2) and to identify the corresponding emotion from a seven-forced-choice response format. We measured the percentage of accuracy, the reaction time (RT), and the confidence level (CL) in the perceived accuracy of the raters’ responses. We found the highest MDS-UPDRS 3.2 scores in PD, and higher in AD than HCs. When evaluating the posed expression captures, raters identified a lower percentage of correct answers in the PD and AD groups than HCs. There was no difference in raters’ response accuracy between the PD and AD. No difference was observed in RT and CL data between groups. Hypomimia in patients correlated positively with the global MDS-UPDRS-III and negatively with Mini Mental State Examination scores. PD and AD patients have a similar pattern of reduced facial emotion expressivity compared to controls. These findings hold potential pathophysiological and clinical implications.
The effect of parenting behaviours on adolescents’ rumination: a systematic review of longitudinal studies
Authors: Castro Tiago , Pinto Tiago Miguel , Morais Ana , Costa Raquel , Jongenelen Inês , Lamela Diogo 
Published: October 2023

AbstractRumination is an emotional regulation mechanism strongly associated with the development and maintenance of internalising psychopathology in adolescence and adulthood. Parenting behaviours (PBs) play a pivotal role in the development of rumination in children and adolescents. Nonetheless, the specific PBs that can either protect against or increase the risk of rumination development remain poorly understood. This systematic review aimed to explore the (1) temporal associations between PBs and adolescents’ rumination and (2) potential moderators influencing these associations. We conducted a comprehensive search across Web of Science, Scopus, PubMed, Academic Search Complete and Eric databases, adhering to PRISMA reporting guidelines. Out of 1,868 abstracts screened, 182 articles underwent full-text examination, with nine meeting the inclusion criteria for the systematic review. Overall, the studies indicated that PBs characterised by criticism, rejection and control were positively associated with the development of rumination in adolescents, whilst PBs marked by authoritative practises exhibited a negative association with rumination. Gender, temperament, environmental sensitivity and pubertal timing emerged as significant moderators in the effects of PBs on rumination. However, conclusions were limited due to the studies’ methodological heterogeneity. Future studies on PBs and rumination should address various dimensions of PBs and different moderators to identify factors that can modify the development of rumination across adolescence. Findings may inform family-based prevention programmes to promote emotion regulation in adolescents as a protective factor against internalising psychopathology across adulthood.
Preconditioning of discrete state- and control-constrained optimal control convection-diffusion problems
Authors: Dravins Ivo , Neytcheva Maya 
Published: October 2023

AbstractWe consider the iterative solution of algebraic systems, arising in optimal control problems constrained by a partial differential equation with additional box constraints on the state and the control variables, and sparsity imposed on the control. A nonsymmetric two-by-two block preconditioner is analysed and tested for a wide range of problem, regularization and discretization parameters. The constraint equation characterizes convection-diffusion processes.
eGFR slope as a surrogate endpoint for end-stage kidney disease in patients with diabetes and eGFR > 30 mL/min/1.73 m2 in the J-DREAMS cohort
Authors: Sugawara Yuka , Kanda Eiichiro , Ohsugi Mitsuru , Ueki Kohjiro , Kashihara Naoki , Nangaku Masaomi 
Published: October 2023

Abstract
Background
An analysis of European and American individuals revealed that a reduction in estimated glomerular filtration rate (eGFR) slope by 0.5 to 1.0 mL/min/1.73 m2 per year is a surrogate endpoint for end-stage kidney disease (ESKD) in patients with early chronic kidney disease. However, it remains unclear whether this can be extrapolated to Japanese patients.

Methods
Using data from the Japan diabetes comprehensive database project based on an advanced electronic medical record system (J-DREAMS) cohort of 51,483 Japanese patients with diabetes and a baseline eGFR ≥ 30 mL/min/1.73 m2, we examined whether the eGFR slope could be a surrogate indicator for ESKD. The eGFR slope was calculated at 1, 2, and 3 years, and the relationship between each eGFR slope and ESKD risk was estimated using a Cox proportional hazards model to obtain adjusted hazard ratios (aHRs).

Results
Slower eGFR decline by 0.75 mL/min/1.73 m2/year reduction in 1-, 2-, and 3-year slopes was associated with lower risk of ESKD (aHR 0.93 (95% confidence interval (CI) 0.92–0.95), 0.84 (95% CI 0.82–0.86), and 0.77 (95% CI 0.73–0.82), respectively); this relationship became more apparent as the slope calculation period increased. Similar results were obtained in subgroup analyses divided by baseline eGFR or baseline urine albumin-creatinine ratio (UACR), with a stronger correlation with ESKD in the baseline eGFR < 60 mL/min/1.73 m2 group and in the baseline UACR < 30 mg/gCre group.

Conclusion
We found that changes in the eGFR slope were associated with ESKD risk in this population.
Diagnostic biopsy does not accurately reflect the PD-L1 expression in triple-negative breast cancer
Authors: Zdrenka Marek , Kowalewski Adam , Borowczak Jędrzej , Łysik-Miśkurka Joanna , Andrusewicz Hanna , Nowikiewicz Tomasz , Szylberg Łukasz 
Published: October 2023

AbstractPD-L1 expression is known to predict the benefits of immune checkpoint inhibitor therapy for triple-negative breast cancer (TNBC). We examined whether the PD-L1 expression evaluated in biopsy specimens accurately reflects its expression in the whole tumor. Immunohistochemistry was performed on 81 biopsy and resection specimens from patients with TNBC to determine their PD-L1 status. We found PD-L1-positive tumors in 23 (28%) biopsy specimens and primarily PD-L1-negative tumors in 58 (72%). The PD-L1 status was reevaluated in matching postoperative specimens of primarily PD-L1-negative tumors. Of them, 31% (18/58) were positive, whereas 69% (40/58) were negative. Considering the pre- and postoperative analyses, 41 (51%) patients had PD-L1-positive tumors, while 40 had PD-L1-negative tumors. We found 18 (22%) more PD-L1-positive tumors while examining the resection specimens compared to biopsies, and the difference was statistically significant (p = 0.0038). Diagnostic biopsies do not fully reflect the PD-L1 expression in TNBC. Our results suggest that a significant subset of TNBC patients may be misclassified as PD-L1-negative and disqualified from anti-PD-L1 therapy.
Improved crop productivity and soil properties under varying planting densities of Pentaclethra macrophylla Benth. and Acacia auriculiformis A. Cunn. in Congo Basin
Authors: Mapenzi Neville , Katayi Alain L. , Bauters Marijn , Masimane Jules , Schure Jolien , Kweyu Raphael , Nabahungu Nsharwasi L. 
Published: October 2023

AbstractSoil fertility depletion is a major constraint to agricultural production in the Congo Basin and is aggravated by climate change. Therefore, agroforestry systems (AFS) are used to improve soil fertility and crop productivity. Indeed,Pentaclethra macrophyllaandAcacia auriculiformisare among the most agroforestry species used in this basin. However, smallholders are limited in their choice of agroforestry species and the spacing. Hence, this study comparatively evaluated the effects ofP. macrophylla(native) andA. auriculiformis(exotic) legume tree species on soil macronutrients and food crops’ yield during four cropping seasons. To do this, a multifactor trial design was implemented in Lobilo catchment, Congo Basin, with two tree species, four tree planting densities (T1: 2500 trees × ha−1, T2: 625 trees × ha−1, T3: 278 trees × ha−1; and T0: crop monoculture), and three intercrops (cassava, maize, and peanut). The results revealed that both agroforestry species did not significantly differ regarding their impact on soil macronutrients. The total nitrogen and the pH in soil have increased with the cropping seasons. With regard to the food crops, cassava and maize yield were greater underP. macrophyllathan underA. auriculiformis.However, the 2500 trees × ha−1density negatively affected food crops’ yield and did not allow food crop production after the second cropping season. To maximize the positive effects of these AFS, it is essential to plant or co-plant these two agroforestry species at 625 trees × ha−1density. Future research could investigate other native species and include water and light interactions.
Assessing concrete nest boxes for cavity-nesting bees
Authors: Henry Mickaël , Berrou Pierre-Jules , Bourdon Sarah , Guilbaud Laurent , Vaissière Bernard E. 
Published: October 2023

AbstractArtificial nest boxes for solitary bees and other cavity-nesting Hymenoptera are increasingly used for a variety of purposes, including ecological research, crop pollination support and public outreach. Their attractivity and colonization success by cavity-nesting solitary bees depend on their design and placement, including hole dimensions, orientation and the neighboring habitats and available resources. While most bee nest boxes are made of wooden materials, we assessed here the suitability of perennial, concrete nest boxes for cavity-nesting bees. We carried out a three-year nesting survey of 52 custom-made nest boxes located in 11 different sites throughout France and totaling 2912 available holes of 6, 8, 10 or 12 mm in diameter. Concrete nest boxes successfully attracted reproductive females of solitary bee species and supported successful larval development until the emergence of new individuals. Preferred cavities were the smallest ones (6-8 mm), located at the lowest tested positions above ground (31-47 cm) and oriented southward. Local bee populations established in nest boxes steadily increased throughout the three successive seasons in nearly all study sites. The cavity-nesting bee communities were mostly composed of rather common and generalist species, but also comprised a foraging specialist. Additionally, two cleptoparasitic bee species were detected. All species belonged to the Megachilidae. We further discuss the effects of neighboring urban and natural habitats as potential source or sink of nesting bees, as well as opportunities of concrete nest boxes as tools for urban agriculture and more generally for the new biomimetic urban designs to restore local ecosystem services in cities.
Patient Knowledge and Trust in Health Care. A Theoretical Discussion on the Relationship Between Patients’ Knowledge and Their Trust in Health Care Personnel in High Modernity
Authors: Conradsen Stein , Vardinghus-Nielsen Henrik , Skirbekk Helge 
Published: October 2023

AbstractIn this paper we aim to discuss a theoretical explanation for the positive relationship between patients’ knowledge and their trust in healthcare personnel. Our approach is based on John Dewey’s notion of continuity. This notion entails that the individual’s experiences are interpreted as interrelated to each other, and that knowledge is related to future experience, not merely a record of the past. Furthermore, we apply Niklas Luhmann’s theory on trust as a way of reducing complexity and enabling action. Anthony Giddens’ description and analysis of the high modern society provides a frame for discussing the preconditions for patient-healthcare personnel interaction. High modernity is dominated by expert systems and demands trust in these. We conclude that patient knowledge and trust in healthcare personnel is related because both knowledge and trust are future- and action-oriented concepts. The traits of high modernity provides opportunities and challenges as the personnel can and must perform discretion. This discretion must be made in a context where knowledge is considered uncertain and preliminary.
Finitary Type Theories With and Without Contexts
Authors: Haselwarter Philipp G. , Bauer Andrej 
Published: October 2023

AbstractWe give a definition of finitary type theories that subsumes many examples of dependent type theories, such as variants of Martin–Löf type theory, simple type theories, first-order and higher-order logics, and homotopy type theory. We prove several general meta-theorems about finitary type theories: weakening, admissibility of substitution and instantiation of metavariables, derivability of presuppositions, uniqueness of typing, and inversion principles. We then give a second formulation of finitary type theories in which there are no explicit contexts. Instead, free variables are explicitly annotated with their types. We provide translations between finitary type theories with and without contexts, thereby showing that they have the same expressive power. The context-free type theory is implemented in the nucleus of the Andromeda 2 proof assistant.