bluuebunny/crossref_metadata_2025_split huggingface dataset:
Group 3 Innate Lymphoid Cells Exacerbate Lupus Nephritis by Promoting B Cell Activation in Kidney Ectopic Lymphoid Structures
Authors: Li Feng , Liang Zhou , Zhong Haojie , Hu Xinrong , Tang Ziwen , Zhu Changjian , Shen Jiani , Han Xu , Lin Ruoni , Zheng Ruilin , Tang Ruihan , Peng Huajing , Zheng Xunhua , Mo Chengqiang , Chen Peisong , Wang Xin , Wen Qiong , Li Jianbo , Xia Xi , Ye Hongjian , Qiu Yagui , Yu Jianwen , Fu Dongying , Liu Jiaqi , Wang Rong , Xie Huixin , Guo Yun , Li Xiaoyan , Fan Jinjin , Liu Qinghua , Mao Haiping , Chen Wei , Zhou Yi 
Published: November 2023

AbstractGroup 3 innate lymphoid cells (ILC3s) represent a new population in immune regulation, yet their role in lupus nephritis (LN) remains elusive. In the present work, systemic increases in ILC3s, particularly in the kidney, are observed to correlate strongly with disease severity in both human and murine LN. Using MRL/lpr lupus mice and a nephrotoxic serum‐induced LN model, this study demonstrates that ILC3s accumulated in the kidney migrate predominantly from the intestine. Furthermore, intestinal ILC3s accelerate LN progression, manifested by exacerbated autoimmunity and kidney injuries. In LN kidneys, ILC3s are located adjacent to B cells within ectopic lymphoid structures (ELS), directly activating B cell differentiation into plasma cells and antibody production in a Delta‐like1 (DLL1)/Notch‐dependent manner. Blocking DLL1 attenuates ILC3s’ effects and protects against LN. Altogether, these findings reveal a novel pathogenic role of ILC3s in B cell activation, renal ELS formation and autoimmune injuries during LN, shedding light on the therapeutic value of targeting ILC3s for LN.
Thermal tolerance exceedances of an endangered unionid mussel in the Rio Grande Basin, with implications for river management
Authors: Rangaswami Xenia L. , Kiser Alexander H. , Ramey Matthew , Stoeckel James A. , Berg David J. , Lopez Roel R. , Randklev Charles R. 
Published: November 2023

Abstract

Freshwater mussels are globally imperilled, which is attributed to their sensitivity to changes in streamflow and temperature. The Black River in south‐eastern New Mexico, USA, harbours a stronghold population of the federally endangered unionid Popenaias popeii (Texas hornshell). Decreasing discharge and elevated water temperatures are considered the primary factors responsible for the species’ decline. However, the impact of these factors has not been quantified directly, which may hinder conservation efforts.
The upper thermal tolerances (LT05 and LT50) of Black River P. popeii larvae (glochidia) and newly transformed juveniles were evaluated. Individuals were acclimated to 27°C and then immersed at five experimental temperatures (28, 30, 32, 34 and 36°C) for 24 h (glochidia) or 96 h (juveniles). The thermal tolerances of P. popeii and its presumed host fishes were then overlain onto in situ water temperature and discharge data to determine thermal exceedances in the past 15 years. Temperature was hindcast back to 2007 using machine learning (random forest model) and coupled with discharge.
For glochidia, LT05 was exceeded frequently (41%) and LT50 was exceeded occasionally (13%) during spring and summer periods, whereas juvenile thresholds were never exceeded. Upper continuous duration above threshold (UCAT) analysis revealed periods of catastrophic high temperature, which were used to determine discharge bottlenecks.
This study demonstrates how laboratory‐derived physiological thresholds can be used in conjunction with environmental data to evaluate the hydrological needs of aquatic organisms, which is useful in efforts to maintain flow regimes that protect native ecosystems.
Nickel‐CatalyzedRegiodivergent Acylzincation of Styrenes with Organozincs andCO†
Authors: Wang Chenglong , Liu Ning , Wu Xianqing , Qu Jingping , Chen Yifeng 
Published: November 2023

Comprehensive SummaryTransition metal‐catalyzed carbometallation of unsaturated hydrocarbons constitutes one of the most efficient synthetic methodologies for the construction of C—C bond. Recently, the incorporation of organometallic reagent with the CO gas as a nucleophilic acyl synthon could enable the acylmetallation reaction, which greatly increases the horizon of carbometallation chemistry. Herein, we report a nickel‐catalyzed regiodivergent acylzincation ofo‐cyano cinnamate ester ando‐cyano styrene, in which the cyano moiety intramolecularly captures zinc intermediates to trigger the tandem cyclization process. This protocol features mild conditions, broad substrate scope and excellent functional group tolerance, thus affording a diverse array of highly functionalized carbocyclic compounds.
Quality and shelf life evaluation of gluten‐free blends and cake premix
Authors: Singh Gurwinder , Kaur Kamaljit 
Published: November 2023

AbstractBakery premixes are composed of a blend of ingredients that offer convenient and quick baking solutions. In the present study, four blends were prepared by taking buckwheat, chickpea, plantain flour, and rice flour in the ratio of (40:20:10:30) T1, (40:20:20:20) T2, (40:20:30:10) T3, and (40:20:40:0) T4. Cake premixes were formulated by adding sugar, baking powder, baking soda, corn starch, and glycerol monostearate in the ratio of 60:5:2.5:1:2 in all the four blends. Blends were analyzed for physicochemical, functional, thermal, bioactive, and antioxidant properties, and premixes were evaluated for cake baking quality. The T3 premix was selected based on evaluated parameters, and the prepared cake contained about 5.02% protein, 27.70% fat, and 1.22% fiber. Textural attributes of cake from the premix were comparable to nongluten cakes available in the market. The cake premix T3 remained acceptable in terms of quality with progression of the storage period of 120 days in aluminum laminates under ambient conditions. During storage, the moisture content decreased from 9.52% to 6.72%, the free fatty acid increase was up to 0.117%, and the peroxide value increased up to 0.48 meq O2/kg. The developed gluten‐free premix will provide nutritional, economic, and better texture attributes to the target segment of consumers.
Efficacy of Whole‐Blood Model of Gadolinium‐Based Contrast Agent Relaxivity in Predicting Vascular MR Signal Intensity In Vivo
Authors: Norris Evan C. , Schneider Guenther , Clark Toshimasa J. , Kirchin Miles A. , Wilson Gregory J. , Maki Jeffrey H. 
Published: November 2023

BackgroundPrevious in vitro studies have described sub‐linear longitudinal and heightened transverse H2O relaxivities of gadolinium‐based contrast agents (GBCAs) in blood due to their extracellular nature. However, in vivo validation is lacking.PurposeValidate theory describing blood behavior of R1 and R2* in an animal model.Study TypeProspective, animal.Animal ModelSeven swine (54–65 kg).Field Strength/Sequence1.5 T; time‐resolved 3D spoiled gradient‐recalled echo (SPGR) and quantitative Look‐Locker and multi‐echo fast field echo sequences.AssessmentSeven swine were each injected three times with 0.1 mmol/kg intravenous doses of one of three GBCAs: gadoteridol, gadobutrol, and gadobenate dimeglumine. Injections were randomized for rate (1, 2, and 3 mL/s) and order, during which time‐resolved aortic 3D SPGR imaging was performed concurrently with aortic blood sampling via an indwelling catheter. Time‐varying [GBCA] was measured by mass spectrometry of sampled blood. Predicted signal intensity (SI) was determined from a model incorporating sub‐linear R1 and R2* effects (whole‐blood model) and simpler models incorporating linear R1, with and without R2* effects. Predicted SIs were compared to measured aortic SI.Statistical TestsLinear correlation (coefficient of determination, R2) and mean errors were compared across the SI prediction models.ResultsThere was an excellent correlation between predicted and measured SI across all injections and swine when accounting for the non‐linear dependence of R1 and high blood R2* (regression slopes 0.91–1.04, R2 ≥ 0.91). Simplified models (linear R1 with and without R2* effects) showed poorer correlation (slopes 0.67–0.85 and 0.54–0.64 respectively, both R2 ≥ 0.89) and higher averaged mean absolute and mean square errors (128.4 and 177.4 vs. 42.0, respectively, and 5506 and 11,419 vs. 699, respectively).Data ConclusionIncorporating sub‐linear R1 and high first‐pass R2* effects in arterial blood models allows accurate SPGR SI prediction in an in vivo animal model, and might be utilized when modeling MR blood SI.Level of Evidence1Technical EfficacyStage 1
Hepatitis A outbreak in a refugee shelter in Kiel, northern Germany
Authors: Krumbholz Andi , Marcic Anne , Valentin Malte , Schemmerer Mathias , Wenzel Jürgen J. 
Published: November 2023

AbstractIn the spring of 2023, three Ukrainian war refugees from a municipal community shelter and a volunteer caregiver at an affiliated daycare center in Kiel, Germany, were diagnosed with infectious jaundice attributable to a single hepatitis A virus (HAV) subgenotype IA strain. Similar HAV sequences have been observed in Germany and other European countries for several years. One refugee and the volunteer required hospitalization. Four children were asymptomatically infected but excreted high levels of HAV ribonucleic acid in the stool. The infections were probably acquired in Germany, but a source could not be determined. The outbreak was contained through vaccination, increased hygiene, and education. The existing HAV vaccination recommendation for refugee shelter staff and volunteers should be consistently implemented.
L‐Theanine alleviates heat stress through modulation of gut microbiota and immunity
Authors: Liu Sha , Wang Bin , Lin Ling , Xu Wei , Gong Zhi‐Hua , Xiao Wen‐Jun 
Published: November 2023

AbstractBACKGROUNDHeat stress (HS) damages the intestines, disrupting gut microbiota and immune balance. l‐Theanine (LTA), found in tea, alleviates oxidative stress and cell apoptosis under HS; however, its effects on gut microbiota and immunity under HS remain unclear. To investigate this, we administered LTA doses of 100, 200, and 400 mg·kg−1·d−1 to C57BL/6J mice. On day 44, the model group and LTA intervention group were subjected to continuous 7‐day HS treatment for 2 h per day.RESULTSThe results demonstrated that LTA intervention improved food intake, body weight, and intestinal epithelium, and reduced the water intake of heat‐stressed mice. It increased the abundance of Turicibacter, Faecalibaculum, Bifidobacterium, and norank_f_Muribaculaceae, while reducing that of Lachnoclostridium and Desulfovibrio. LTA intervention also increased the concentrations of amino acid and lipid metabolites, regulated macrophage differentiation stimulated by gut microbiota and metabolites, reduced the antigen presentation by macrophages to the specific immune system, promoted B‐cell differentiation and sIgA secretion, inhibited pro‐inflammatory factors, and enhanced intestinal defense. Mechanistically, LTA downregulated heat shock protein 70 expression and the TLR4/NF‐κB/p38 MAPK signaling pathway, restoring gut microbiota and immune balance.CONCLUSIONWe suggest that LTA can alleviate HS by modulating gut microbiota, metabolites, and immunity, indicating its potential as a natural active ingredient for anti‐HS food products. © 2023 Society of Chemical Industry.
Digestibility of corn distiller's dried grains with solubles (DDGS) in practical feeds with phytase and xylanase treatment fed to Nile Tilapia
Authors: Welker Thomas L. , Sealey Wendy , Tan Ronnie 
Published: November 2023

Abstract

Objective
Distiller's dried grains with solubles (DDGS), a by-product of ethanol production from cereal grains, has seen limited use by fish feed manufacturers as an alternative protein source. Over the past 20 years, advances and greater uniformity in processing methods have improved the quality of DDGS and, likely, its digestibility by fish. Our objective was to reevaluate the digestibility of corn DDGS by Nile Tilapia Oreochromis niloticus using a present-day commercial product with and without xylanase and phytase enzyme treatment.

Methods
Apparent digestibility coefficients (ADCs) for the major proximate nutrients (dry matter, crude protein, crude fat, ash, digestible energy, and phosphorus) were determined using a practical, commercial-type feed that was formulated and processed with DDGS supplied at 30% as weight : weight substitution (DDGS diet) of the reference diet (Ref diet). After drying, Ref and DDGS diets were top-coated with phytase (3310 FTU/kg) + xylanase (7665 BXU/kg) enzymes (Ref+ and DDGS+) and compared with their untreated dietary counterparts.

Result
Digestibility of crude protein was similar among diets and unaffected by enzyme treatment. For dry matter and digestible energy, ADC values were significantly lower for the DDGS diet, but enzyme addition improved digestibility to that of the Ref diets. Apparent digestibility for crude fat and ash was significantly better for the DDGS feed, with ash digestibility improved after treatment with xylanase + phytase for both Ref+ and DDGS+. For phosphorus, digestibility was similar among the Ref, Ref+, and DDGS diets, but with enzyme treatment, the phosphorus ADC increased significantly in DDGS+.

Conclusion
Results of this digestibility trial show that corn DDGS is a highly digestible protein source for use in feeds of Nile Tilapia at 30% of diet. Generally, apparent digestibility of proximate nutrients was either better or similar in DDGS when compared with the reference diet and could be further improved with the addition of xylanase + phytase.
Sex differences in lower urinary tract function in mice with or without spinal cord injury
Authors: Hashimoto Mamoru , Karnup Sergei , Daugherty Stephanie L. , Cho Kang Jun , Banno Eri , Shimizu Nobutaka , Fujita Kazutoshi , Hirayama Akihide , Uemura Hirotsugu , de Groat William C. , Beckel Jonathan M. , Yoshimura Naoki 
Published: November 2023

AbstractObjectivesWe examined sex differences of lower urinary tract function and molecular mechanisms in mice with and without spinal cord injury (SCI).MethodsSCI was induced by Th8−9 spinal cord transection in male and female mice. We evaluated cystometrograms (CMG) and electromyography (EMG) of external urethral sphincter (EUS) at 6 weeks after SCI in spinal intact (SI) and SCI mice. The mRNA levels of Piezo2 and TRPV1 were measured in L6‐S1 dorsal root ganglia (DRG). Protein levels of nerve growth factor (NGF) in the bladder mucosa was evaluated using an enzyme‐linked immunosorbent assay.ResultsSex differences were found in the EUS behavior during voiding as voiding events in female mice with or without SCI occurred during EUS relaxation periods without EUS bursting activity whereas male mice with or without SCI urinated during EUS bursting activity in EMG recordings. In both sexes, SCI decreased voiding efficiency along with increased tonic EUS activities evident as reduced EUS relaxation time in females and longer active periods of EUS bursting activity in males. mRNA levels of Piezo2 and TRPV1 of DRG in male and female SCI mice were significantly upregulated compared with SI mice. NGF in the bladder mucosa showed a significant increase in male and female SCI mice compared with SI mice. However, there were no significant differences in Piezo2 or TRPV1 levels in DRG or NGF protein levels in the bladder mucosa between male and female SCI mice.ConclusionsWe demonstrated that female and male mice voided during EUS relaxation and EUS bursting activity, respectively. Also, upregulation of TRPV1 and Piezo2 in L6‐S1 DRG and NGF in the bladder could be involved in SCI‐induced lower urinary tract dysfunction in both sexes of mice.
New therapeutic targets to prevent benign prostatic enlargement and symptomatic progression to benign prostatic obstruction—ICI‐RS 2023
Authors: Kanai Anthony , Chakrabarty Basu , Winder Michael , Hashim Hashim , Wein Alan , Abrams Paul , Fry Christopher 
Published: November 2023

AbstractAimsBenign prostatic enlargement (BPE) can impact lower urinary tract function due to its potential progression to benign prostatic obstruction (BPO). Treatment options include removal of the obstruction by surgery or through use of therapeutics designed to slow growth or reduce tissue stress imposed by muscular stromal components. Inflammation and development of fibrosis can also raise intrinsic tissue stress within the gland, further impacting obstruction. Outflow tract obstruction can also impact emission and ejaculation if the obstruction persists.MethodsThis review summarizes an ICI‐RS think tank considering novel drug treatments that might address BPO caused by progressive development of BPE, as well as manage decompensation changes to bladder function.ResultsTopics included recent advances in our understanding of pathological changes occurring to the prostate and other lower urinary tract tissues during progressive development of BPE, and how prevention or reversal might benefit from the identification of novel drug targets. These included contractile properties of prostatic tissues, the impact of BPE and its effects on bladder function, the deposition of intramural fibrotic tissue with protracted BPO, the role of inflammation in the development of BPE and its progression to BPO. In particular, we discussed current therapeutic options for treating BPE/BPO, and new therapeutic targets, what they treat and their advantage over current medications.ConclusionSeveral new drug targets were identified, including soluble guanylate cyclase (sGC), the receptor for nitric oxide (NO•), and sGC activators that promotes sGC‐mediated cGMP production when sGC is inactivated and unresponsive to NO•.
Effect of inorganic particles gradation on the properties of high‐filled unsaturated polyester resin/CaCO3 composites
Authors: Liu Zhonglin , Liu Wanyu , Ma Lanyu , Mo Min , Huang Yifeng , Lai Wenqin , Xu Mengxue , Li Yanming , Mo Qiufeng , Bai Xingjia , Chen Cheng , Huang Zhimin , Zheng Yihua 
Published: November 2023

AbstractThe preparation of high‐filled composites by compounding with calcium carbonate (CaCO3) and polymer has received much attention. However, challenges such as enhancing the interaction between CaCO3 and the polymer and improving the performance of the resulting composites need to be addressed. In this paper, composites with a high filler content were made by adding fillers of various CaCO3 gradations into unsaturated polyester resin (UPR). The mechanical properties, curing degree, component distribution, and structural phases of resulting blends were analyzed by a universal testing machine, differential scanning calorimeter, Raman, and scanning electron microscope/energy‐dispersive x‐ray spectroscopy (EDX), respectively. Based on the research findings, optimizing the CaCO3 gradation significantly increased the strength of the resulting blends. The average flexural strength increased by 343.5%, while the average compressive strength increased by 888.9%. The composites with an optimized gradation of filler showed a significant reduction in uncured domains, leading to an improvement in the curing degree of the composites. The interfacial defects between the optimized graded CaCO3 filler and the UPR were reduced, resulting in a uniform distribution with no visible interface. The Raman and EDX analyses show that the gradation optimization technique has successfully prevented structural phase separation and enhanced the tendency to agglomerate between CaCO3 and UPR. This study proposes an effective method for developing high‐performance UPR/CaCO3 composites with a high filler content.Highlights
The optimized gradation of CaCO3 was used as filler to address the weak interaction of CaCO3 with the UPR in a high‐filled UPR/CaCO3 composite system while improving the performance of the composite.
The gradation optimization of CaCO3 filler can improve the morphology and reduce the porosity of the high‐filled UPR composites.
The gradation optimization of CaCO3 filler can effectively improve the curing effect of UPR.
Optimizing the CaCO3 gradation resulted in a significant increase in the average flexural strength (by 343.5%) and average compressive strength (by 888.9%) of the resulting blends.
Perovskite solar cell technology scaling‐up: Eco‐efficient and industrially compatible sub‐module manufacturing by fully ambient air slot‐die/blade meniscus coating
Authors: Vesce Luigi , Stefanelli Maurizio , Rossi Federico , Castriotta Luigi Angelo , Basosi Riccardo , Parisi Maria Laura , Sinicropi Adalgisa , Di Carlo Aldo 
Published: November 2023

AbstractThe efficiency gap between perovskite (PVSK) solar sub‐modules (size ≥200 cm2) and lab scale cells (size ˂1 cm2) is up to 36%. Moreover, the few attempts present in the literature used lab‐scale techniques in a glove‐box environment, reducing its compatibility for further product industrialization. Here, we report a PVSK sub‐module (total area 320 cm2, aperture area 201 cm2, 93% geometrical fill factor [GFF]) fabricated in ambient air by hybrid meniscus coating techniques assisted by air and green antisolvent quenching method. To suppress nonradiative recombination losses, improve carrier extraction and control the PVSK growth on such a large surface, we adopted phenethylammonium iodide (PEAI) passivation and PVSK solvent addiction strategies. The high homogeneous and reproducible layers guarantee an efficiency of 16.13% (7% losses with respect to the small area cell and zero losses with respect to the mini‐modules) and a stability of more than 3000 h according to International Summit on Organic PV Stability, dark storage/shelf life in ambient (ISOS‐D‐1). The sustainability of used methods and materials is demonstrated by the life cycle assessment. The scale‐up operation allows for strong impact mitigation in all the environmental categories and more efficient consumption of the resources. Finally, the economic assessment shows a strong cost reduction scaling from mini‐ to sub‐module (about 40%).
Probability of success and group sequential designs
Authors: Grieve Andrew P. 
Published: November 2023

AbstractIn this article, I extend the use of probability of success calculations, previously developed for fixed sample size studies to group sequential designs (GSDs) both for studies planned to be analyzed by standard frequentist techniques or Bayesian approaches. The structure of GSDs lends itself to sequential learning which in turn allows us to consider how knowledge about the result of an interim analysis can influence our assessment of the study's probability of success. In this article, I build on work by Temple and Robertson who introduced the idea of conditional probability of success, an idea which I also treated in a recent monograph.
Requirement elicitation model for global software development vendors
Authors: Yaseen Muhammad 
Published: November 2023

AbstractRequirements elicitation is critical phase of software development that involve direct communication of clients and vendors. In global software development (GSD), importance of requirements elicitation becomes more critical due to geographical distance. The aim of this research is to design and implement requirement elicitation model (REM) where GSD organization can evaluate their organizations and can classify their level of implementation where organization lies. Systematic literature review (SLR) was conducted for the identification of critical success factors (CSFs) from a sample of 70 research articles. Furthermore, the identified CSFs are organized into five levels based on Capability Maturity Model Integration (CMMI) and Software Outsourcing Vendors' Readiness Model (SOVRM). A second SLR was conducted for identifying all possible practices for the implementation of CSFs. To validate the findings of SLR and to find more practices in the view of industrial practitioners for the identified CSFs, a questionnaire survey was conducted in the GSD organizations in which 35 experts, from different countries, contributed. For REM evaluation, we have conducted two case studies. The outcome analysis after conducting case studies confirms that our assessment framework is easier to understand, apply, and successfully critic the strengths and weaknesses of organizations during requirements elicitation in GSD.