bluuebunny/crossref_metadata_2025_split huggingface dataset:
LKB1‐SIK2 loss drives uveal melanoma proliferation and hypersensitivity to SLC8A1 and ROS inhibition
Authors: Proteau Sarah , Krossa Imène , Husser Chrystel , Guéguinou Maxime , Sella Federica , Bille Karine , Irondelle Marie , Dalmasso Mélanie , Barouillet Thibault , Cheli Yann , Pisibon Céline , Arrighi Nicole , Nahon‐Estève Sacha , Martel Arnaud , Gastaud Lauris , Lassalle Sandra , Mignen Olivier , Brest Patrick , Mazure Nathalie M , Bost Frédéric , Baillif Stéphanie , Landreville Solange , Turcotte Simon , Hasson Dan , Carcamo Saul , Vandier Christophe , Bernstein Emily , Yvan‐Charvet Laurent , Levesque Mitchell P , Ballotti Robert , Bertolotto Corine , Strub Thomas 
Published: November 2023

AbstractMetastatic uveal melanomas are highly resistant to all existing treatments. To address this critical issue, we performed a kinome‐wide CRISPR‐Cas9 knockout screen, which revealed the LKB1‐SIK2 module in restraining uveal melanoma tumorigenesis. Functionally, LKB1 loss enhances proliferation and survival through SIK2 inhibition and upregulation of the sodium/calcium (Na+/Ca2+) exchanger SLC8A1. This signaling cascade promotes increased levels of intracellular calcium and mitochondrial reactive oxygen species, two hallmarks of cancer. We further demonstrate that combination of an SLC8A1 inhibitor and a mitochondria‐targeted antioxidant promotes enhanced cell death efficacy in LKB1‐ and SIK2‐negative uveal melanoma cells compared to control cells. Our study also identified an LKB1‐loss gene signature for the survival prognostic of patients with uveal melanoma that may be also predictive of response to the therapy combination. Our data thus identify not only metabolic vulnerabilities but also new prognostic markers, thereby providing a therapeutic strategy for particular subtypes of metastatic uveal melanoma.
Caregiver Language Preference and Health Care Utilization Among Children With Asthma
Authors: Emmanuel Mickey , Margolis Rachel , Badh Ranjodh , Kachroo Nikita , Teach Stephen J. , Parikh Kavita 
Published: November 2023

BACKGROUND AND OBJECTIVES
Asthma is a leading cause of health care utilization in children and disproportionately affects historically marginalized populations. Yet, limited data exist on the role of caregiver language preference on asthma morbidity. The study aim was to determine whether caregiver non-English language preference (NELP) is associated with unscheduled asthma-related health care utilization in pediatric patients.

METHODS
This was a retrospective cohort study using data from a population-level, disease-specific registry of pediatric patients with asthma living in the District of Columbia (DC). Patients aged 2 to 17 years were included and the study period was 2019. The primary exposure variable was language preference: English preferred (EP) or NELP by self-identified language preference. The primary outcome was unscheduled asthma-related health care utilization including emergency department visits, hospitalizations (ICU and non-ICU), and ICU visits alone. Logistic regression was used to calculate adjusted odds ratios (aORs).

RESULTS
Of the 14 431 patients included, 8.1% had NELP (1172 patients). In analyses adjusted for age, sex, ethnicity, insurance status, diagnosis of persistent asthma, controller prescription, and encounter with a primary care provider, caregiver NELP was associated with an increased odds of having an asthma-related emergency department visit (aOR, 1.37; 95% CI, 1.08–1.74), hospitalization (aOR, 1.79; 95% CI, 1.18–2.72), and ICU visit (aOR, 4.37; 95% CI, 1.93–9.92). In the Hispanic subgroup (n = 1555), caregiver NELP was associated with an increased odds of having an asthma-related hospitalization (aOR, 1.73; 95% CI, 1.02–2.93).

CONCLUSIONS
In the population of children in the District of Columbia with asthma, caregiver NELP was associated with increased odds of asthma-related health care utilization, suggesting that caregiver language preference is a significant determinant of asthma outcomes.
Comparative evaluation of low-level laser therapy and multi-wave locked system laser therapy for carpal tunnel syndrome: a retrospective study
Authors: ÜŞEN Ahmet , AKYOL Ahmet , TOPAL İlknur 
Published: November 2023

Purpose: This study aimed to evaluate the effects of Low-Level Laser Therapy (LLLT) and Multi-Wave Locked System (MLS) Laser Therapy on clinical and functional parameters in Carpal Tunnel Syndrome.
Methods: In the study, 60 patients with mild-moderate carpal tunnel syndrome were evaluated retrospectively in 3 groups. Group I; LLLT, Group II; MLS Laser therapy, Group III; It consisted of patients who did not receive any laser application and were given splints, a home exercise program and patient education. Visual Analogue Scale (VAS), Hand Grip Strength measurement and BOSTON Carpal Tunnel Questionnaire measurements were evaluated from the patients' files at baseline, 4th week and 12th week.
Results: Statistically significant improvements were observed in all parameters in Group I and Group II at 4 weeks and 12 weeks compared to baseline. Group III showed no significant differences in grip strength measurements and the BOSTON Functional Status Scale at 4 weeks compared to baseline. The changes between baseline and 4 weeks were statistically significantly better in Group I and Group II compared to Group III for all parameters. No significant difference was found between Group I and Group II in VAS and grip strength improvements, but the improvement in Boston Symptom Severity Scale was significantly more prominent in Group II than in Group I.
Conclusion: Both LLLT and MLS Laser Therapy can be considered as conservative treatment options along with splinting and exercise to control the symptoms of Carpal Tunnel Syndrome. MLS Laser Therapy may be superior to LLLT in reducing symptom severity.
Five-week of solution-focused group counseling successfully reduces internet addiction among college students: A pilot study
Authors: Pu Yu , Liu Yuting , Qi Yawei , Yan Ziyou , Zhang Xinhe , He Qinghua 
Published: November 2023

AbstractBackground and AimsIn the digital age, Internet addiction (IA) was deemed an epidemic and few treatments had been effectively developed for it. Here, we proposed a solution-focused group counseling (SFGC) as a potentially solution to reduce Internet addiction among college students. The present study examined the short- and long-term effect of a five-week solution-focused group counseling intervention on Internet addiction.MethodsThirty-two participants were recruited and randomly assigned to either the experimental or control group, and twenty-six participants completed the whole intervention. The experimental group (n = 14) received the intervention, while control group (n = 12) did not. The revised version of the Chinese Internet Addiction Scale (CIAS-R), the Future Time Perspective, and resting-state EEG were administered pre-intervention, post-intervention, and at two follow-up tests (one month and six months after intervention).ResultsThe results showed that the scores of the CIAS-R in the experimental group were significantly decreased after intervention, and these effects could be sustained for one month and six months follow-ups. Additionally, the intervention conducted an increase in future time perspective. EEG results further suggested that the alpha, beta, and gamma absolute power decreased after the intervention.ConclusionThese results from the pilot-study primarily suggested that solution-focused group counseling could be an effective intervention for Internet addiction.
Effect the pre-exposure prophylactic of hydroxychloroquine on severe COVID-19 disease: a randomized controlled trial
Authors: Valizadeh Mahnaz , Tarjoman Termeh , Farhoudi Behnam , Chouhdari Arezoo , Mesgarian Masoumeh , SeyedAlinaghi SeyedAhmad , Zangeneh Mehrangiz , Hanifezadeh Zahra , Adain Atashi Hesam , Massumi-Naini Hamidreza , Abolghasemi Shahla , Dezfulinejad Manije , Haghani Shima 
Published: November 2023

Background. In vitro studies have shown some effects for Hydroxychloroquine (HCQ) against SARS-CoV-2 virus. Despite effective vaccination program, relatively large proportion of population remains unvaccinated. So, there still remains a need for other prophylactic measures. The present study aims to evaluate whether HCQ can prevent severe COVID-19 outcomes among health-care workers. Materials and methods. In this randomized, double blind placebo-controlled clinical trial 334 healthcare workers aged 18–65 years old were included of whom 278 individuals completed the study. Participants were randomly assigned to the HCQ group (800 mg at day one, followed by 400 mg weekly for the next 7 weeks); or the placebo group. Participants were followed three weeks after the last dose of drug or placebo (10 weeks from the first dose of drug or placebo). The primary outcome was hospitalization or death from COVID-19. Results. Of 148 people who received HCQ, none were hospitalized or died from COIVD-19, while of 130 people who received the placebo, 2 (1.5%) were hospitalized for COIVD-19 (p-value: 0.26). And, 22 (14.9%) people in the HCQ group and 15 (11.6%) people in the placebo group contracted COVID-19 (p-value: 0.99). Adverse reactions were reported by 5 (3.4%) of participants in the HCQ group and 5 (3.9%) of participants in the placebo group (p-value: 0.99).
Conclusion. We found that HCQ has no significant prevention effect on the incidence of mild COVID-19. However, the low rate of hospitalization (the primary outcome) in this trial like most of the other clinical trials with HCQ would have required increasing the sample size considerably to be able to comment on the effectiveness of HCQ in prevention of severe forms including death rate. This justifies systematic reviews to include similar studies to further investigate the issue.
Automated monitoring of pollen quality in Douglas fir using digital images: applications for handling and storage
Authors: Rubio-Plascencia Cassandra , Sánchez-Salgado Mara Lizbeth , García-Campusano Florencia , Muñoz-Gutierrez Liliana 
Published: November 2023

Background: Conservation and breeding programs for forest species require efficient pollen quality monitoring strategies to maximize collection, storage and pollination efforts.
Question: Can the ImageJ image analysis software be implemented to efficiently monitor pollen viability and germinability for quality testing?
Studied species: Pseudotsuga menziesii (Mirb.) Franco
Methods: Tetrazolium staining and germination on 10 % Brewback media was used to test Douglas-fir pollen quality. Two macros for automated counting were developed with ImageJ free software using digitized microscopic images, and were tested to evaluate provenances, germination in different media, dehydration/rehydration and freeze-storage treatments, and finally to compare pollen quality between mature trees and GA-induced strobili from juvenile trees.
Results: Automated monitoring of pollen quality was feasible because the software suitably distinguishes viable from non-viable pollen grains due to differential tetrazolium staining that translates into pixel density, whereas germinability was assayed based on pollen size and circularity. This tool was used to guide the selection of appropriate pollen handling conditions and compare pollen production in adult and GA-induced juvenile trees. Pollen flash-freezing and storage at -80 °C showed recovery between 74 and 98 % of its initial germinability after rehydration; however, viability detected by tetrazolium is reduced, suggesting that handling and storage affect the reliability of the test.
Conclusions: The flexibility of the Fiji software allowed the development of an efficient and accurate macro for monitoring P. menziesii pollen quality, reducing invested time and effort involved, which supports future research on its conservation physiology and its successful application for germplasm production.
Analisis Perbandingan Tutupan Lahan (Land Cover) Wilayah Malang Raya Menggunakan Citra Sentinel
Authors: Nadzirah Rufiani , Indarto Indarto , Widyaningsih Dini Retno , Okiawan Muhammad Ilwan Tegih 
Published: November 2023

Abstrak. Wilayah Malang Raya merupakan salah satu wilayah dengan pertumbuhan penduduk yang cepat di Jawa Timur. Selain jumlah pertumbuhan penduduk yang besar, wilayah Malang juga memiliki potensi wisata dan termasuk daerah dengan pusat pendidikan di Jawa Timur. Hal tersebut berpengaruh besar terhadap pertumbuhan pembangunan yang terjadi di wilayah Malang Raya. Saat ini informasi mengenai tutupan lahan mulai banyak dibutuhkan, salah satunya adalah untuk mengetahui ketersediaan lahan terbuka yang dapat digunakan dalam sektor pertanian. Informasi mengenai tutupan lahan dapat memanfaatkan teknologi penginderaan jauh. Tujuan dari penelitian ini adalah untuk mengetahui perbandingan tutupan lahan di Wilayah Malang Raya selama periode 2015 dan 2020 menggunakan Citra Satelit Sentinel dan metode Maximum Likelihood Classification (MLC). Analisis yang telah dilakukan menghasilkan peta tematik tutupan lahan Wilayah Malang Raya tahun 2015 dan 2020. Peta tematik yang dihasilkan pada tahun 2015 memiliki kualitas citra yang kurang baik karena terdapat tutupan awan dan kabut. Pada tahun 2020 menghasilkan kualitas citra yang lebih baik. Hasil klasifikasi yang dilakukan menggunakan metode Maximum Likelihood menunjukkan bahwa pada tahun 2015 nilai Overall sebesar 87.81% dan Kappa 85.79%. Pada tahun 2020 menghasilkan nilai Overall sebesar 91.88% dan Kappa sebesar 90.56%. Perbandingan tutupan lahan periode tahun 2015 dan 2020 menunjukkan hasil peningkatan terbesar terjadi pada kelas tutupan tegalan/ladang. Sedangkan penurunan terbesar terjadi pada kelas tutupan kebun. Comparative Analysis of Land Cover in the Malang Regional Area Using Sentinel ImageryAbstract. The Greater Malang region is experiencing the swiftest population growth in East Java. In addition to substantial population expansion, the Malang area possesses tourism potential and serves as an educational hub in East Java. This growth significantly influeces the region's development. Comprehensive information about land coverage is essential, including determining the availability of open land for agricultural use. Remote sensing technology facilitates obtaining land coverage data. This study compares land coverage in the Malang region during 2015 and 2020, utilizing Sentinel Satellite Imagery and the Maximum Likelihood Classification (MLC) method. The analysis produced thematic maps of land coverage for the Greater Malang Region in 2015 and 2020. The 2015 thematic maps suffered from poor image quality due to cloud cover and fog, which improved in 2020. The Maximum Likelihood classification results indicated an overall accuracy of 87.81% and a Kappa value of 85.79% for 2015, improving to 91.88% and 90.56% in 2020, respectively. A comparison of land cover for the 2015 and 2020 periods shows that the most significant increase occurred in wasteland/heterogeneous agricultural field areas, while the most significant decrease ensued in plantation areas.
Globalización y bilingualización en Colombia
Authors: Miranda Montenegro Iván Ricardo 
Published: November 2023

La presente obra es el resultado de un trabajo de investigación que indaga desde una perspectiva glotopolítica, sobre el papel de la lengua en el contexto de políticas públicas en el sistema educativo colombiano. La bilingualización se entiende como el proceso de instauración de un modelo educativo para el bilingüismo cuyo discurso circulante desde la institucionalidad viene estableciendo unas representaciones sociales positivas asociadas al anhelo del país en ser aceptado por la comunidad globalizada. Los conceptos sobre discurso y su función determinadora y determinada a partir de las interacciones comunicativas en la formación, transformación, mantenimiento y supresión de algunas representaciones sociales que proporcionan legitimidad aparente al proceso de participación en la internacionalización de mercados, asigna sustento teórico al estudio. Colateralmente, el ejercicio discursivo oficial sobre la implementación de un bilingüismo educativo empieza también a generar un proceso de reconfiguración de nuevas identidades que socavan las identidades regionales y nacional. El análisis crítico del discurso (Fairclough, 1989, 1992, 1995, 2006; Van Dijk, 2004, 2009; Wodak, 2003) posibilitó el discernimiento sobre el rol del discurso oficial en búsqueda de la aceptación social de la política de bilingüismo. El Ministerio de Educación Nacional colombiano se ha encargado de circular un discurso impregnado de poder institucionalizado que ejerce coerción sobre un actor social pasivizado, que impulsa el desconocimiento de un país tradicional y naturalmente multilingüe, en procura de un bilingüismo artificial. La reproducción del discurso sobre educación bilingüe en Colombia, según el estudio, devela un sesgo sociopolítico que se inclina hacia las determinaciones transcendentales que favorecen los procesos de internacionalización mercantilista, y minimiza los procedimientos de salvaguarda y fortalecimiento cultural regional. La representación de institucionalidad, la necesidad de ser bilingües y la identidad supranacional junto con la mediación del currículo en el marco de la globalización, sugieren un cambio de arquetipo identitario.
Sheathless radial approach in contemporary coronary rotational atherectomy: data from two high-volume centers
Authors: Dall’Ara Gianni , Alagna Giulia , Spartà Daniela , Compagnone Miriam , Grotti Simone , Guerrieri Giuseppe , Campanella Francesca , Taverna Giovanni , Galvani Marcello , Tarantino Fabio , Andò Giuseppe 
Published: November 2023

Aim: To analyze the feasibility and procedural outcome of percutaneous coronary intervention (PCI) using rotational atherectomy (RA), performed via transradial sheathless guiding catheter, as compared to a standard radial and femoral vascular approach.
Methods: All consecutive patients undergoing RA at two high-volume PCI centers from May 2011 to May 2023 were included. Comparisons were made between the two transradial approaches and between the three types of vascular access.
Results: Two hundred twenty-three patients were enrolled. Baseline characteristics were similar, with the exception of gender. We observed, in percentages, fewer cases of failure for sheathless than standard transradial attempts (7.5% vs. 11.5%, respectively), whereas all trans-femoral attempts were successful. Transfemoral procedures were longer and more frequently performed under mechanical circulatory support. There was no difference in procedural success between the three vascular approaches. A trend towards a higher rate of vascular and bleeding complications was found in the femoral group.
Conclusion: Sheathless transradial vascular access is a viable option when performing RA during complex PCI procedures, tending to result in fewer failures than the standard transradial approach and reduced bleeding and vascular complications compared to the femoral method.
Exploring the Structure and Properties of V<sub>w</sub>Se<sub>y</sub>Te<sub>2–y </sub>Mixed Crystals in the VTe2–VSe2 System
Authors: Kurig Sophia , Ketter Fabian , Frommelius Anne , Hakala B. Viliam , Leusen Jan van , Friese Karen , Dronskowski Richard 
Published: November 2023

Vanadium(IV) chalcogenide materials are of increasing interest for use in catalysis and energy conversion-related applications. Since no ternary compounds are yet known in the V–Se–Te system, we studied ternary VwSeyTe2–y (w = 1.10, 1.13; y = 0.42, 0.72) phases crystallizing in space group P3m1 (no. 164). Two single-crystal specimens with differing compositions of a solid solution were obtained using the ceramic method. All products were characterized by either single-crystal or powder X-ray diffraction. The lattice parameters increase with rising tellurium content in accordance to the larger ionic radius of the tellurium anion compared to selenium. The chemical compositions were confirmed by energy dispersive X-ray spectroscopy. Further, magnetic measurements revealed mostly antiferromagnetic properties. Simultaneous differential scanning calorimetry/thermogravimetric analyses in nitrogen atmosphere showed endothermic decomposition accompanied by the formation of VN. The results of this work can serve as a basis for the synthesis of new phases in the V–Se–Te and related vanadium chalcogenide systems.
Genetic Determinants of Virulence of Two Clones of the Maize Streak Virus
Authors: Edema Richard , Gordon Donald T 
Published: November 2023

Using two infectious DNA clones originating from isolates obtained in Kenya, a genetic investigation of maize streak virus (MSV) pathogenicity was carried out. The susceptible Pioneer hybrid 3379 was used to investigate the virulence of kernels administered through vascular puncture inoculation. Mild [pMSV-KL (mild)] and severe [pMSV-Km(severe)] parental infectious MSV DNA clones were linked with considerable changes in the severity of chlorotic streaks and stunting of seedlings. Using restriction endonuclease fragments, chimeric clones were created from parental clones in order to investigate the genetic elements influencing these changes. Restrictions mapping was used to validate clone identities.
For genomic portions encoding the replication initiator (Rep and RepA), movement (MP), and coat (CP) protein ORFs as well as for those containing non-coding long (LIR) and short intergenic regions (SIR), putative virulence determinants were discovered. Recombinant clones with intermediate symptoms included LIRs together with the first 189 nt of the Rep/RepA ORFs from the pMSV-Km (Severe) and pMSV-KL (Mild) ORFs. Symptoms identical to those of parental clones were produced by complementary substitution of the Rep/RepA ORF. When compared to pMSV-Km (Severe), the recombinant clone having MP, CP, and SIR genome sequences from pMSV-KL(Mild) and LIR, Rep, and RepA genome sequences from pMSV-Km (Severe) displayed much more severe symptoms and accumulated greater concentrations. The reciprocal clone, in contrast, displayed much milder signs and virus titers than pMSV-KL(Mild). The level of leaf chlorosis and plant stunting were linked with viral accumulation.
These findings are consistent with the idea that MSV virulence is a polymorphic feature involving complementary sense genes (LIR and SIR) and the virion.
Karadeniz’in Su Ürünleri Yetiştiriciliği Sektörü Açısından Potansiyeli ve Türk Somonu İhracatının Finansal ve Ekonomik Analizi
Authors: Erol Sercan , Erüz Coşkun 
Published: November 2023

Bu çalışmanın amacı, Türk somonu olarak sunulan gökkuşağı alabalığının üretim ve gelir potansiyelini	değerlendirmek ve yeni hedef dış pazarlara yönelik olarak ürün çeşitlendirme ve sürdürülebilir büyüme açısından alınması gereken önlemler sıralanmaktır. Bu kapsamda su ürünleri yetiştiricilik sektörüne ilişkin Türkiye Cumhuriyet Merkez Bankası A-Tarım, Ormancılık ve Balıkçılık ana sektörü 032-Su ürünleri yetiştiriciliğine ait 2021 yıllı konsolide gelir tablosu ve bilanço verileri, Türk somonuna ilişkin Doğu Karadeniz İhracatçılar Birliği verileri kullanılmıştır. Analiz sonuçları, Türk su ürünleri yetiştiriciliği sektörünün 2021 yılında bir önceki yıla göre %54 arttığı görülmüştür. Ancak, sektör yüksek finansal kaldıraç, sermaye piyasası araçlarından yeterince yararlanılmamış ve borç verenlerin baskısı gibi zorluklarla karşı karşıyadır. Türk somonu açısından ise DKİB verilerine göre 2022 yılında 37 ülkeye 49 bin ton ihracat gerçekleştirildiği ve 2019 yılına kıyasla 7 kat artarak toplamda 363 milyon USD gelir elde edilmiştir. Bu durum Türk somonunun markalaşması ve tanıtımı ile Türk somonuna yönelik yapılan yatırım ve desteklerin etkinliği vurgulanmaktadır.