bluuebunny/crossref_metadata_2025_split huggingface dataset:
A modern approach to the treatment of scoliosis with skewed pelvic bones by correcting asymmetric mobile planovalgus deformity of the feet
Authors: Vinderlikh M. E. , Shchekolova N. B. 
Published: January 2024

Introduction. The treatment of combined pathology of posture and feet remains an urgent problem of the child population.Target. To study the effectiveness of using an individually made wedge-shaped corrective heel pad for each foot in the treatment of grade I-II scoliosis with pelvic bone distortion due to asymmetric mobile flat-valgus deformity of the feet for 3 years.Object and methods. A comparison group and a main group were formed from 39 children and adolescents aged 3 to 15 years with grade I-II scoliosis with skewed pelvic bones and mobile flat-valgus deformity of the feet; patients underwent orthopedic examination every 6 months, radiography of the spine with pelvic bones, photoplantography, measurement of the pronation angle of the calcaneus, the study of quality of life indicators.Results. There was a decrease in clinical symptoms in 54.2 % of patients of the main group with regression of the II degree of scoliosis to the I degree, the pronation angle of the calcaneal bones decreased by 4.25 ± 0.78 ° with the preservation of minimal asymmetry after 3 years, a decrease in the difference of the iliac crests by 3 .64 ± 1.59 mm and the angle of scoliotic deformity of the spine on radiographs by 5.22 ± 1.36°. Correlation analysis showed a statistically significant strong and moderate direct relationship between the pronation angle of the left calcaneus and the angle of scoliotic spinal deformity on radiographs in the 1st and 3rd years of the study, as well as with the difference in the iliac crests throughout the study. The quality of life of patients of the main group with correction of the asymmetry of foot hyperpronation improved by 15.5 %, in the comparison group by 2.9 %.Conclusions. The results of the study confirmed the effectiveness of the use of individual wedge-shaped corrective heel pads for each foot in the treatment of grade I-II scoliosis with skewed pelvic bones due to asymmetric mobile flat-valgus deformity of the feet.
Modern visualization diagnostic methods of non-invasive breast carcinomas (review of literature)
Authors: Maksimov D. A. , Morozov A. M. , Penyaz' E. V. , Rogovenko V. V. , Belyak M. A. 
Published: January 2024

Relevance. Breast carcinomas (BC) remain one of the most actual problems of modern oncology. According to statistics, the incidence of BC is steadily increasing, making it the most common cancer pathology among women. In this situation, the aspect of diagnosing BC at early, non-invasive stages, is certainly important, which still reduces mortality, increases the possibility of organ-preserving treatment, duration and quality of life of patients.The purpose of study. The purpose of study is to investigate and perform a comparative analysis of imaging techniques for the diagnosis of non-invasive breast carcinomas.Materials and methods. Modern Russian and foreign literature about the diagnosis of non-invasive breast carcinomas was analyzed. Publications not older than 7 years published in specialized medical editions were taken into account Results. Not only main, but also new, promising imaging modalities that are not currently part of routine practice were analyzed. The main imaging patterns in non-invasive breast carcinomas (DCIS, LCIS and Paget's breast cancer), feasibility and prognostic value of certain diagnostic methods in different nosologies of this disease were also discussed.Conclusion. The statistics provided explain the importance of breast carcinomas problem, as well as the relevance of its diagnosis at non-invasive stages. According to the authors, the issue of accurate diagnosis of breast cancer in situ is subject to further discussion and study, but we would like to note that when non-invasive breast carcinoma is suspected and doubtful imaging results are obtained, one should not limit oneself only to routine methods of imaging studies, but expand further diagnostic tactics until accurate results are obtained and a final diagnosis is made.
Autological bone marrow concentrate for the treatment of early stages of femoral head avascular necrosis (literature review)
Authors: Panin M. A. , Zagorodnii N. V. , Boiko A. V. , Petrosyan A. S. 
Published: January 2024

Relevance. In the treatment of the early stages of the femoral head osteonecrosis core decompression is widely used, which reduces intraosseous pressure and increases blood flow to the femoral head. To increase the efficiency of the core decompression the use of various bioadjuvants, including concentrate of autologous bone marrow cells is needed, the appropriateness of which is actively discussed.Purpose of the systematic review: evaluation of the effectiveness of autologous bone marrow cells in core decompression in the treatment of the early stages of the femoral head osteonecrosis according to the literature.Material and methods. Articles with clinical data in full text in English or Russian available in the Internet (PubMed and elibrary databases) were included in the study. Articles containing actual data on the stage of osteonecrosis, the number of femoral heads operated on, the use of autologous bone marrow cells as an adjunct to the core decompression of the femoral head, the number of ineffective surgical interventions after which total hip arthroplasty was required were included.Results. Sixteen original articles (2011–2022) were analyzed, and the results of 1135 femoral heads treatment (892 with autologous bone marrow cells, 243 without autologous bone marrow cells, control group) were evaluated. Patient follow-up periods ranged from 9 to 120 months, averaging 45 months. When using autologous bone marrow cells, the need for total hip arthroplasty was observed in 168 of 892 patients (18.8 %); when core decompression was used without autologous bone marrow cells, the rate of total hip arthroplasty was 27.2 % (p > 0.05). Only 2 articles out of 16 authors did not consider autologous bone marrow cells to be reasonable in the core decompression of femoral head.Conclusion. The use of autologous bone marrow cells concentrate as a bioadjuvant in the core decompression slows the progression of osteonecrosis. According to the overwhelming number of authors, the core decompression using autologous bone marrow cells is highly effective, significantly reducing the intensity of pain syndrome and improving the function of the hip joint. The results will be more encouraging with the further development of regenerative medicine, which will provide new samples of cellular preparations for bone tissue structure restoration.
Dynamics of stress-index and spectral indicators of cardiointervalogram of elite skiers-racers in the preparatory, competition and transition periods depending on the volume and intensity of training loads
Authors: Kataev D. A. , Tsirkin V. I. , Trukhin A. N. , Trukhina S. I. 
Published: January 2024

Acutality: Heart rate variability, allows you to assess the state of the autonomic nervous system, which is useful for studying the condition of athletes, including endurance athletes, for example, ski racers. The stress index indicator is often used to assess the impact of stress on a person. It is shown that the stress index is closely correlated with the intensity of metabolism and with the indicators of energy consumption of the body.Objective. To provide information on the values of the stress index for athletes depending on a number of factors, including their sports specialization, and for ski racers on the volume and intensity of the training load during the preparatory, competitive, transitional periods of the ski season.Materials and methods. The literature analysis in the Pubmed database for the last 10 years in English and Russian languages has been carried out.Conclusion. A review of the literature on the magnitude of the stress index showed that athletes who train for endurance, this indicator is low, especially among ski racers, if it is compared with the magnitude of the index in representatives of other sports, as well as with healthy people who do not play sports. There is no information about the value of the stress index in athletes in foreign literature, but there is such information in the domestic literature, which allowed us to present this review, which also includes the results of our research.
Modern approaches to the diagnosis and treatment of biliary complications after liver transplantation (literature review)
Authors: Magomedov K. M. , Novruzbekov M. S. , Gulyaev V. A. , Lutsyk K. N. , Kazymov B. I. , Alekberov K. F. , Akhmedov A. R. , Anosova Е. Yu. , Yaremin B. I. 
Published: January 2024

Relevance. The choice of biliary anastomosis is the main factor determining the risk of developing biliary complications after orthotopic liver transplantation. The two most common forms of biliary tract reconstruction are choledocholedochostomy (duct-to-duct anastomosis) and choledochoeunostomy (connection of the bile duct to the jejunum). The choice of biliary tract reconstruction is determined by a variety of factors, including the underlying pathology of the liver, the size of the bile ducts of the donor and recipient, previous transplantation or previous biliary tract surgery, as well as the preferences of the surgeon performing the operation. Despite the correctly chosen method of reconstruction, complications such as strictures, lithiasis, biloma, and bile leakage occur infrequently.Objective: to present a review of the literature on methods of diagnosis and treatment of biliary complications after liver transplantation.Materials and methods. The analysis of literary sources in English and Russian from 2010 to 2023 on this topic in the databases PubMed, MEDLINE, Google Scholar was carried out. The review highlights clinical studies, as well as literature reviews on similar topics with an emphasis on the treatment and diagnosis of biliary complications.Conclusion. The problem of biliary complications of liver transplantation remains relevant and unresolved to the end. Noninvasive imaging techniques for complications arising after biliary tract surgery have prospects for development. The tactics of percutaneous, endoscopic and open interventions on the biliary graft tree require further improvement.
Müller kası rezeksiyon cerrahisi: Objektif ve yeni bir method ile cerrahi başarının değerlendirilmesi
Authors: YOLCU Demet , YAYLACİOGLU TUNCAY Fulya 
Published: January 2024

Amaç: Çalışmanın amacı, 6mm veya 8mm eksizyonla gerçekleştirilen Müller kası konjonktival rezeksiyon (MMCR) cerrahisi sonrası oto-refraktometrenin (AR) ön monitör görüntülerini (FMI) kullanarak üst göz kapağı pozisyonundaki değişiklikleri değerlendirmektir.
Yöntem: Ocak 2020-Nisan 2023 tarihleri arasında 6mm veya 8mm MMCR cerrahisi uygulanan hastaların tıbbi kayıtları geriye dönük olarak incelendi. Üst göz kapağı yüksekliği (Margin reflex distance: MRD1) AR-FMI ve Image J yazılımı kullanılarak değerlendirildi. Cerrahi öncesi ve sonrası takiplerde alınan AR-FMI’ler incelenerek üst göz kapağı pozisyonu (AR-MRD1) belirlendi.
Bulgular: Çalışmaya dahil edilen 34 kişiden 14'üne 6mm, 20'sine ise 8mm MMCR cerrahisi uygulandı. Cerrahi öncesi ortalama AR-MRD1 değeri 6mm MMCR grubu için 2,1 0,8 mm; 8mm MMCR grubu için 2,30,7 mm idi ve gruplar arasında anlamlı fark yoktu (p:0,45). Cerrahi sonrası AR-MRD1 6mm rezeksiyon grubunda 8mm rezeksiyon grubuna göre daha yüksekti ancak fark anlamlı değildi (6mm MMCR grubu için 3,90,5 mm ve 8mm MMCR grubu için 3,60,7 mm idi) (p:0,14). Ortalama AR-MRD1 farkı 6mm rezeksiyon grubunda 1,4  0,2 mm ve 8 mm rezeksiyon grubunda 1,10,3 mm idi ancak fark anlamlı değildi (p.0,09).
Sonuç: MMCR cerrahi başarısını etkileyen faktörler incelenmektedir ve daha güvenilir sonuçlar elde etmek için daha objektif ve standartlaştırılmış MRD ölçüm yöntemine ihtiyaç vardır. Mevcut çalışma sonuçlarına göre 6mm veya 8mm MMCR ameliyatı ile benzer göz kapağı yüksekliği elde etmek mümkün olup, kolay değerlendirilebilen ve uygun maliyetli bir cihaz kullanılarak cerrahi sonucu analiz etmek mümkündür.
Complex Systems Modelling: A Systematic, Quantitative Systems Engineering Approach
Authors: Slater David , Hill Rees 
Published: January 2024

In his Cynefin classification of system behaviours, Snowden (2007), posits that his Simple (Clear ) and even Complicated system categories are sufficiently understandable to be amenable to the traditional methods of system analysis and modelling. Here, relationships between entities in the system are clear and unambiguous and they behave predictably. So, we can build mathematical “models” to describe how the system is expected to function in different situations.
Leaving aside his fourth category, Chaotic, as too hard for the moment, this paper focusses on the third category, Complex systems. Yaneer Bar-Yam [1998] defined &quot;complex systems&quot; as systems that &quot;have multiple interacting components, whose collective behaviour cannot be simply inferred from the behaviour of components.&quot;
So, the traditional system modelling approach of decomposition into components and attempting to build up a picture of system performance from individual pieces, alone, is no longer appropriate for this class. The problem is that most of the methods we currently employ, and particularly the methods that attempt to make the case that these systems are safe, (such as Fault trees, FMEA. and Root Cause Analysis), rely on this decomposition into components and attempt to make the parts more reliable individually. Even the more systemic approaches, such as Reason’s (1990) Swiss Cheese Model, and Leveson’s System Theoretic Analysis (STPA) (2004), encourage adding layers of protection, barriers, strengthened control loops and better safety critical systems, in the hope that they are making the systems safer. But without a valid model to test the effectiveness of these add-ons, we cannot be sure that they will not have an opposite effect, of making the systems even more complex, unreliable and unpredictable.
For complex systems, the careful gathering and documentation of system components and their relationships is still a necessary first step, but we need an additional capacity to model these complex systems to test out and prove they work safely and successfully in the real world. (not just draw fixed component relationships as system “models” (wiring diagrams))
In the last 10 years, an original approach developed by Hollnagel, (2012), to model systems as sets of interactive, interdependent “functions”, (abstracted from and independent of component details), has now been developed to the point where it can take the basic data and structures from the current component focussed system engineering “models”, such as MBSE( Goldberg (1994)), and can pull it all together in a dynamic system model from which analysts can discern how it really works in practice, and predict the emergent behaviours characteristic of complex systems.
The paper describes how this methodology developed and how it is currently used to model real situations, to analyse incidents and anticipate events.
Severe Type 2 Inflammation Leads to High PAF-Associated Pathology in Chronic Rhinosinusitis with Nasal Polyps (CRSwNP): Hierarchal Cluster Analysis Using Bulk RNA Barcoding and Sequencing
Authors: Ishino Takashi , Oda Takashi , Kawasumi Tomohiro , Takemoto Kota , Nishida Manabu , Horibe Yuichiro , Chikuie Nobuyuki , Taruya Takayuki , Hamamoto Takao , Ueda Tsutomu , Takeno Sachio 
Published: January 2024

Platelet-activating factor (PAF) is a phospholipid-derived inflammatory mediator, triggering various inflammatory conditions, including eosinophil activation and recruitment. Despite the unclear mechanism underlying the formation and maintenance of nasal polyp in chronic rhinosinusitis with nasal polyps (CRSwNP), there is strongly indication of its association with type 2 inflammation. This study aimed to quantify PAF-associated gene expression, examining enzymes involved in PAF synthesis (lysophosphatidylcholine acyltransferase 1 (LPCAT1), LPCAT2, LPCAT3, and LPCAT4), and PAF degradation (PAF acetylhydrolases 1 B2 (PAFAH1B2), PAFAH1B3, and PAF acetylhydrolases 2 (PAFAH2)), and PAF receptor (PTAFR). Transcriptomic analysis encompassed CRSwNP, including eosinophilic CRS (ECRS) (n=9), nonECRS (n=8), and a subgroup of ECRS with aspirin exacerbated respiratory disease (Asp) (n=3) based on the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis (JESREC) criteria. Gene expression profiles from nasal polyps in CRSwNP patients and the uncinate process in normal subjects (controls) (n=6) were analyzed using bulk RNA barcoding and sequencing (BRB-seq). Hierarchal analysis, utilizing the average linkage method with iDEP1.1, clarified the relationship between Differentially Expressed Genes (DEG) expressions and cluster formation. Two clusters (cluster 1 (n=11) and cluster 2 (n=9)) of CRSwNP divided by hierarchal analysis showed distinct gene expression profiles in the specific pathway of “cytokine-cytokine receptor interaction” in KEGG pathway and upregulated type 2 inflammatory gene expression in cluster 2. In CRSwNP subclassification of ECRS, nonECRS, and Ctrl, PTAFR was only upregulated in ECRS and nonECRS. In cluster analysis, cluster 1 showed significant downregulation of LPCAT2 and upregulation of PTAFR expression, while cluster 2 showed significant upregulation of LPCAT1, PAFAH1B2, and PTAFR and downregulation of PAFAH2 expression. These findings suggest that cluster 2, characterized by severe type 2 inflammatory gene expression, manifest a prominent PAF-associated pathophysiology. Understanding this high PAF-associated pathophysiology in cluster 2 could provide valuable insights into the treatment and management of CRSwNP.
Migrate3D: Software for simplified post-tracking analysis of 3D and 2D cell migration data
Authors: Mynar Emily R. , Kinahan Matthew W. , Thali Markus , Symeonides Menelaos 
Published: January 2024

Abstract

Summary
: Migrate3D is a cell migration analysis tool whose purpose is to computationally process positional cell tracking data generated via other image acquisition/analysis software and generate biologically meaningful results. The functionalities of Migrate3D include step-based calculations of each cell track, single-cell-level summary statistics, mean squared displacement analysis, and machine learning-based evaluation of the entire dataset and subpopulations of cells found within it. The parameters calculated within Migrate3D have been previously developed and validated by other groups, and were selected to facilitate extraction of the maximum depth of information possible from input datasets. Variables are user-adjustable to enable customized analyses of diverse motility patterns and cell types, both in three- and two-dimensional timelapse data. Independent of any particular upstream image analysis or cell tracking software, Migrate3D only needs positional data over time to execute the suite of calculations. This presents a unique opportunity to standardize and streamline cell migration analysis.
Availability and Implementation
: Migrate3D is an open-source software suite implemented in Python (version 3.x) and functions on Windows, MacOS, and Linux systems. Source code and detailed installation and usage instructions are available freely at:
Nanoparticle Superlattices with Non-Equilibrium Crystal Shapes
Authors: Macfarlane Robert , Ye Matthew , Hueckel Theodore , Gatenil Perapat , Nagao Keisuke , Carter W 
Published: January 2024

Abstract
Nanoparticle assembly is a materials synthesis strategy that enables precise control of nanoscale structural features. Concepts from traditional crystal growth research have been tremendously useful in predicting and programming the unit cell symmetries of these assemblies, as their thermodynamically favored structures are often identical to atomic crystal analogues. However, these analogies have not yielded similar levels of influence in programming crystallite shapes, which are a consequence of both the thermodynamics and kinetics of crystal growth. Here we demonstrate kinetic control of colloidal crystal shape using nanoparticle building blocks that rapidly assemble over a broad range of concentrations, thereby producing well-defined crystal habits with symmetrically oriented dendritic protrusions and providing insight into the crystals’ morphological evolution. Counterintuitively, these non-equilibrium crystal shapes actually become more common for colloidal crystals synthesized closer to equilibrium growth conditions. This deviation from typical crystal growth processes observed in atomic or molecular crystals is shown to be a function of the drastically different timescales of atomic and colloidal mass transport, and thus this work provides design principles to expand the morphological diversity of nanoparticle superlattice crystal habits beyond the anhedral or equilibrium polyhedral shapes synthesized to date. Finally, we use this insight to synthesize crystallite shapes that have never before been observed, demonstrating the ability to both predict and program kinetically-controlled superlattice morphologies.
Eg5 UFMylation promotes spindle organization during mitosis
Authors: Duan Ranhui , Li Guangxu , Huang Yuanjiang , Wei Liyi , Huang Hongjing , Zhu Yingbao , Xiao Qiao , Zujia Wang , Wen Huang 
Published: January 2024

Abstract

UFMylation is a highly conserved ubiquitin-like post-translational modification that catalyzes the covalent linkage of UFM1 to its target proteins. This modification plays critical roles in the maintenance of endoplasmic reticulum (ER) proteostasis, DNA damage response, autophagy, and transcriptional regulation. Mutations in UFM1, as well as in its specific E1 enzyme UBA5 and E2 enzyme UFC1, have been genetically linked to microcephaly. Our previous research unveiled the important role of UFMylation in regulating mitosis. However, the underlying mechanisms have remained unclear due to the limited identification of substrates. In this study, we identified Eg5, a motor protein crucial for mitotic spindle assembly and maintenance, as a novel substrate for UFMylation and identified Lys564 as the crucial UFMylation site. UFMylation did not alter its transcriptional level, phosphorylation level, or protein stability, but affected the mono-ubiquitination of Eg5. During mitosis, Eg5 and UFM1 co-localize at the centrosome and spindle apparatus, and defective UFMylation leads to diminished spindle localization of Eg5. Notably, the UFMylation-defective mutant of Eg5 (K564R) displayed shortened or asymmetrical spindles, and suppressed cell proliferation in HeLa cells. Overall, Eg5 UFMylation is essential for proper spindle organization, mitotic progression and cell proliferation.