bluuebunny/crossref_metadata_2025_split huggingface dataset:
Linking of errata: Current practices in online physical sciences journals
Authors: Poworoznek Emily L. 
Published: September 2003

AbstractReader awareness of article corrections can be of critical importance in the physical and biomedical sciences. Comparison of errata and corrigenda in online versions of high‐impact physical sciences journals across titles and publishers yielded surprising variability. Of 43 online journals surveyed, 17 had no links between original articles and later corrections. When present, hyperlinks between articles and errata showed patterns in presentation style, but lacked consistency. Variability in the presentation, linking, and availability of online errata indicates that practices are not evenly developed across the field. Comparison of finding tools showed excellent coverage of errata by Science Citation Index, lack of indexing in INSPEC, and lack of retrieval with SciFinder Scholar. The development of standards for the linking of original articles to errata is recommended.
Managing tradeoffs in the electronic age
Authors: Wagner A. Ben 
Published: September 2003

AbstractAn overview of the development of electronic resources over the past three decades is provided, discussing key features, disadvantages, and benefits of traditional online databases and CD‐ROM and Web‐based resources. This analysis of gains and losses as information resources have shifted to the Internet provides a basis for identifying key issues in the decision to shift collections and resources toward purely digital formats. Ownership of content, licensing terms, and the proliferation of user interfaces are especially important and still unresolved concerns.
Intermediary's information seeking, inquiring minds, and elicitation styles
Authors: Wu Mei‐Mei , Liu Ying‐Hsang 
Published: September 2003

AbstractThe current research explores how intermediaries seek information from patrons, in particular by analyzing intermediaries' elicitation utterances through three dimensions—linguistic forms, utterance purposes, and communicative functions—to determine whether indeed any dimension appeared consistently, to be called “elicitation styles.” Five intermediaries from four academic libraries (three national university libraries, one private university library) and one research institute library participated in the study. Thirty patrons with 30 genuine search requests were recruited; thus, 30 patron/intermediary information retrieval interactions making a total of 30 encounters were collected. Video/audio data were taped. Dialogues between patron and intermediary were transcribed. Statistical analysis revealed three types of elicitation styles among the five intermediaries, labeled, (1) situationally oriented, (2) functionally oriented, and (3) stereotyped. This study seeks an explanation for different elicitation styles. Qualitative analysis was applied to investigate “inquiring minds.” An inquiring mind is termed to represent a mentality or tendency that one elicits certain threads of questions influenced by professional beliefs, individual characteristics, tasks, goals, and interactional contexts in conversation. The results of qualitative analysis specified three modes of inquiring minds of the intermediaries, namely: (1) information problem detection, (2) query formulation process, and (3) database instructions.
Effect of feed restriction on Hershberger and pubertal male assay endpoints
Authors: Marty M. Sue , Johnson Keith A. , Carney Edward W. 
Published: September 2003

AbstractBACKGROUND: The Hershberger and male pubertal onset assays have been identified as possible Tier I screening tests to detect endocrine‐active compounds (EACs). Both tests rely on changes in reproductive and/or accessory sex gland (ASG) weights in young animals. Because chemical treatment may affect growth rate, the relationship between body weight and reproductive/ASG weights was examined using feed restriction (FR) to produce a targeted 10% decrease in body weight. METHODS: In the male pubertal onset assay, 23‐day‐old rats (12/group) were given ad lib feed or FR until euthanized at 45, 49, 52, 56, or 59 days of age. Despite a 10% body weight differential, pubertal onset was not significantly delayed and testes weights were conserved. Absolute prostate, ventral prostate, seminal vesicle, epididymides, and liver weights were decreased by FR. Relative weights for the prostate, ventral prostate, and seminal vesicles were similar to controls, but relative epididymides and liver weights still exhibited FR‐mediated changes. In the Hershberger assay, male rats (12/group) castrated at 36 days of age were given ad lib feed or FR in the presence or absence of testosterone propionate (T) from 46–55, 50–59, or 56–65 days of age. At 56, 60, and 66 days of age, rats were euthanized. In untreated animals, FR did not alter absolute ventral prostate, seminal vesicles, or Cowper's gland weights; however, absolute and relative weights of the levator ani‐bulbocavernosus muscles (LABC) were affected. In T‐treated animals, absolute organ weights (the ventral prostate, seminal vesicles, LABC, and glans penis) were relatively insensitive to FR. The weight of the Cowper's gland was affected only at 66 days of age. RESULTS: These data show that reproductive and ASG organ weight endpoints in the Hershberger and male pubertal onset assays can be influenced by FR levels that produce a 10% change in terminal body weight. CONCLUSIONS: The establishment of objective criteria for a positive or negative result is problematic due to the confounding effects of body weight on some endpoints. Furthermore, a 10% decrease in body weight seems to be excessive as a requirement for high‐dose toxicity in these assays due to possible indictment of agents that are not EACs, as well as potential masking of EAC effects coincident with body weight changes. Minimally, caution must be used in interpreting assay results in the presence of a 10% body weight change, recognizing the possible confounding effects of this degree of growth suppression. Birth Defects Res B 68:363–374, 2003. © 2003 Wiley‐Liss, Inc.
Pretreatment with periodate‐oxidized adenosine enhances developmental toxicity of inorganic arsenic in mice
Authors: Lammon Carol A. , Le X. Chris , Hood Ronald D. 
Published: September 2003

AbstractBACKGROUND: Inorganic arsenic, given by injection to pregnant laboratory animals, can induce malformations. Arsenic methylation can be inhibited by periodate‐oxidized adenosine (PAD). Severe human health effects from high chronic arsenic exposure have mainly been reported in populations with significant levels of malnutrition, which may enhance toxicity by diminishing arsenic methylating capacity. This study sought to determine the effect of inhibition of arsenic methylation on the developmental toxicity of arsenic in a mammalian model. METHODS: PAD (100 µM/kg, i.p.), was given to pregnant CD‐1 strain mice 30min before 7.5mg/kg sodium arsenite [As(III)], i.p., or 17.9mg/kg sodium arsenate [As(V)], i.p., on gestation day 8 (GD 8; copulation plug=GD 0). Control dams received As(III), As(V), or PAD alone or were untreated. Test dams were killed on GD 17, and their litters were examined for mortality and gross and skeletal defects. RESULTS: Pretreatment with PAD before either arsenical resulted in increased maternal toxicity and lower fetal weights. Pretreatment also caused higher prenatal mortality, with 8 of 21 and 5 of 17 litters totally resorbed in the PAD plus As(III) and PAD plus As(V) treatment groups, respectively. Significant increases in the incidences of exencephaly, ablepharia, and anomalies of the vertebral centra, sternebrae, and ribs were also associated with PAD pretreatment. Short tail (3 fetuses in 3 litters) was seen only following PAD plus As(III) treatment. CONCLUSIONS: These results demonstrate that the developmental toxicity of inorganic arsenic can be enhanced by PAD, due possibly to inhibited methylation of arsenic. Birth Defects Res B 68:335–343, 2003. © 2003 Wiley‐Liss, Inc.
Comparison of prostate gene expression and tissue weight changes as monitors of antiandrogen activity in GNRH‐inhibited rats
Authors: Nellemann C. , Lefevre P.A. , Ashby J. 
Published: September 2003

AbstractBACKGROUND: The Hershberger assay for antiandrogens and modifiers of steroid biosynthesis uses surgically‐castrated rats. We described an adaptation of the assay using the GnRH inhibitor Antarelix in place of surgical castration [Ashby J, Lefevre PA, Deghenghi R, Wallis N. Regulatory Toxicology and Pharmacology 34:188–203, 2001], and concomitantly described changes in expression of the androgen‐dependent prostatic genes PBP C3, TRPM‐2, and ODC as a possible complement to gravimetric analysis of the sex accessory tissues (SAT) [Nellemann C, Vinggaard AM, Dalgaard M, Hossaini A, Larsen J‐J. Toxicology 163:29–38, 2001]. METHODS: The present study describes the results of combining these two modifications into a single assay. During the course of these experiments it was shown that SD rats gave similar results to AP rats and that the higher stimulatory dose of testosterone propionate (TP) used in our experiments gave stronger assay responses to FLU than the lower dose of TP used by some earlier investigators. The potent antiandrogen flutamide (FLU) and the weak antiandrogen DDE were used to evaluate this modified assay. RESULTS: For all parameters studied (SAT weights and changes in expression of the 3 prostatic genes) FLU gave the expected positive results. The weak antiandrogen DDE gave variable and mainly non‐reproducible responses. Use of DDE as a weak antiandrogen accelerated assessment of the new assay. CONCLUSIONS: Possible reasons for this failure to detect DDE are discussed, and it is concluded that the modified assay is unsuitable for use in its present form. The use of gene expression analyses together with evaluation of SAT weights is a promising tool as an early and sensitive marker of antiandrogen action, but more work is needed on the choice of time frame as well as the selection of genes to monitor. Birth Defects Res B 68:344–354, 2003. © 2003 Wiley‐Liss, Inc.
Maternal‐fetal distribution and prenatal toxicity of 2,2,4‐trimethyl‐1,2‐dihydroquinoline in the rat
Authors: Sitarek Krystyna , Sapota Andrzej 
Published: September 2003

AbstractBACKGROUND: Polnoks R (poly‐2,2,4‐trimethyl‐1,2‐dihydroquinoline) is used as an antioxidant in elastomer processing. It is an embryotoxic and fetotoxic agent. This chemical given per os to female rats induces also teratogenic effect but only at doses toxic to the mother. The aim of the study was to evaluate prenatal development and tissue distribution in rats exposed to 2,2,4‐trimethyl‐1,2‐dihydroquinoline (TMDHQ), a monomer of Polnoks R. METHODS: Females were exposed orally to unlabeled TMDHQ during organogenesis at doses 50–400 mg/kg to asses prenatal toxicity and to radiolabeled 14C monomer at a dose 210 mg/kg to evaluate tissues distribution. RESULTS: TMDHQ administered to pregnant females per os at doses 100 mg/kg and higher produced teratogenic effect (cleft palate, wavy ribs, kyphoscoliosis, exencephaly, external hydrocephalus, hydronephrosis, and renal hypoplasia). Peak 14C‐radioactivity was found in mothers' plasma about 10 hr after administration of this compound at dose 210 mg/kg. The accretion of 14C proceeded with a kinetic constant of 0.35 hr −1 and a half‐life of 53.3 hr. Kidneys are the main organs of monomer excretion. The highest concentration of 14C in maternal tissues 24 hr after oral dosing was found in adipose tissue, sciatic nerve, muscles, kidneys, and liver. Radiocarbon retention in fetuses was the highest in kidneys at all time points after dosing. CONCLUSIONS: This study has demonstrated that transplacental exposure to Polnoks R monomer is teratogenic in rats. 14C retention in placenta, amniotic fluid, and fetal tissues indicates that this compound or its metabolites penetrate into placenta to the fetus. Birth Defects Res B 68:375–382, 2003. © 2003 Wiley‐Liss, Inc.
Gastroschisis is caused by the combination of carbon monoxide and protein‐zinc deficiencies in mice
Authors: Singh Jarnail 
Published: September 2003

AbstractBACKGROUND: Gastroschisis is a rare congenital defect of the abdominal wall. Its occurrence is noted primarily in the offspring of young mothers who often smoke during pregnancy. The incidence of gastroschisis has been increasing in many countries in recent years. The etiology of gastroschisis is not known. METHODS: Pregnant mice of CD‐1 strain were maintained on 17 and 9% protein diets mixed with deficient, normal, or supplemental zinc levels throughout gestation. The dams in each protein‐zinc diet group were randomly divided in two groups. One group was exposed to air (control) and the other to 500 ppm carbon monoxide (CO) in air, in environmental chambers, from gestation days (GD) 8–18. The dams were sacrificed by carbon dioxide asphyxiation on GD 18, and data on malformations was collected. RESULTS: The rates of fetal mortality and malformations were increased by protein and zinc deficiencies. Carbon monoxide exposure also increased fetal mortality. In the low protein group, the rate of fetal mortality was inversely related to the dietary zinc level, and the rate of fetal malformations was highest in the zinc deficient group. The incidence of gastroschisis in the low protein/zinc deficient/CO exposed group was 47%, and 60% of the litters were affected. The incidence of gastroschisis in the rest of the low protein/zinc diets/air or CO groups was 0. CONCLUSION: The data indicates that gastroschisis is caused by the combination of protein‐zinc deficiencies and carbon monoxide exposure during gestation. The finding may be relevant to human populations that experience protein and zinc deficiencies during gestation, and are exposed to CO pollution, or cigarette, or marijuana smoke during pregnancy. Birth Defects Res B 68:355–362, 2003. © 2003 Wiley‐Liss, Inc.
Effects of gestational exposure to ethane dimethanesulfonate in CD‐1 mice: Microtia and preliminary hearing tests
Authors: Tarka‐Leeds Dana K. , Herr David W. , Klinefelter Gary R. , Rogers John M. 
Published: September 2003

AbstractBACKGROUND: Microtia is a reduction in pinna size, usually seen in humans in conjunction with other medical conditions. We report microtia in CD‐1 mice after gestational exposure to ethane dimethanesulfonate (EDS), an alkylating agent and adult rat Leydig cell toxicant. METHODS: Time‐pregnant CD‐1 mice were administered 0, 80, or 160 mg EDS/kg on gestation days (GD) 11–17, or 0 or 160 mg EDS/kg on GD 11–13, GD 13–15 or GD 15–17. Pinnae were measured on postnatal days (PND) 4, 8, 18, and 28; and were observed for detachment from birth through PND 8. Branchial‐arch derived skeletal structures and histology of the pinna was examined on PND 4 and 24. Brainstem auditory evoked response (BAER) tests were carried out at approximately PND 160 to determine possible effects on hearing. RESULTS: All offspring of EDS‐treated dams exhibited bilateral, dose‐related decreases in pinna size. Gestational exposure during GD 11–13 produced smaller ears than during GD 13–15 or 15–17, but not as small as the GD 11–17 regimen. Ossification of other pharyngeal arch derivatives was delayed whereas histology was unremarkable. BAER analysis showed a decrease in the proportion of adult offspring producing a quantifiable response to varied auditory stimuli among EDS‐treated litters. CONCLUSIONS: Gestational exposure to EDS affects pinna development in the mouse, with a broad period of sensitivity during the second half of gestation. Microtia induced by EDS may be associated with hearing deficits, suggesting functional importance of pinna size or additional effects of EDS on ear development not detected by morphological examination. Birth Defects Res B68:383–390, 2003. © 2003 Wiley‐Liss, Inc.
Species comparison of anatomical and functional immune system development
Authors: Holsapple Michael P. , West Lori J. , Landreth Kenneth S. 
Published: September 2003

AbstractThe components of the immune system have not been traditionally emphasized as potential target organs in standard developmental and reproductive toxicity (DART) protocols. A number of workshops have been organized in recent years to examine scientific questions that underlie developmental immunotoxicity tests, and the interpretation of results as they relate to human risk assessment. A key question that must be addressed is to determine the most appropriate species and strains to model the developing human immune system. The objective of this review is to compare the anatomical and functional development of the immune system in several species important to either preclinical studies for drug development or safety assessments for chemicals, with what is known in humans. The development of the immune system in humans will be compared to what is known in mice, rats, dogs and nonhuman primates. Birth Defects Res B 68:321–334, 2003. © 2003 Wiley‐Liss, Inc.
In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase
Authors: Portaccio M. , De Luca P. , Durante D. , Rossi S. , Bencivenga U. , Canciglia P. , Lepore M. , Mattei A. , De Maio A. , Mita D.G. 
Published: September 2003

AbstractThe influence of an extremely low frequency (ELF) magnetic field (50 Hz and 1 mT, EMF) on the activity of a soluble and insoluble horseradish peroxidase (E.C. 1.11.17) has been studied as a function of time. Insoluble derivatives were obtained by enzyme entrapment into two different gelatin membranes or by covalent attachment of the enzyme on two nylon membranes, differently preactivated. Results have shown that the field affects the inactivation rate of the soluble enzyme, while no effects are observed with insoluble derivatives. Since in vivo enzymes are immobilised into the biomembrane bilayer or entrapped into the cytoplasmic mixture, one might speculate that our experimental conditions do not reflect the catalytic activity of the enzymes in vivo. Bioelectromagnetics 24:449–456, 2003. © 2003 Wiley‐Liss, Inc.
Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy
Authors: Ikehara Toshitaka , Yamaguchi Hisao , Hosokawa Keiko , Miyamoto Hiroshi , Aizawa Katsuo 
Published: September 2003

AbstractThe effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorbance of the amide II band, and increased absorbance at around 1600 cm−1. These results suggest that exposure to the ELF magnetic field has reversible effects on the N–H inplane bending and C–N stretching vibrations of peptide linkages, and changes the secondary structures of α‐helix and β‐sheet in cell membrane proteins. Bioelectromagnetics 24:457–464, 2003. © 2003 Wiley‐Liss, Inc.
Power line frequency electromagnetic fields do not increase the rate of protein synthesis in human skin fibroblasts as previously reported
Authors: Shi Biao , Isseroff R. Rivkah , Nuccitelli Richard 
Published: September 2003

AbstractRodemann et al. [Rodemann et al. (1987): Biochem Biophys Res Commun 145:1–9] reported that human skin fibroblasts increase their rate of protein synthesis by as much as over ninefold in response to long term exposure to 20 Hz, 8.4 mT (84 G) magnetic fields. Here we report studies of protein synthesis using an identical cell type, exposure conditions, and the same means of measuring protein synthetic rates. Our initial goal was to determine if the earlier results could be replicated, but we found an inconsistency in the earlier protocol. It exposed cells to [3H]leucine for 6 h prior to measuring incorporation into protein. We found, however, that 24 h is required for [3H]leucine to reach a steady state distribution across the cells' plasma membranes. In addition, we typically measured 100–200 cpm/thousand cells. This is four‐ to eightfold higher than the 19–28 cpm/1000 cells previously reported. Using these conditions, we could find no significant difference in protein synthesis rates between control cells and cells exposed for up to three weeks in an identical electromagnetic field. In addition, we investigated the effects of a 60 Hz field since that is the frequency used for electric power distribution in the United States. Again, we could find no significant effect of this field on rates of protein synthesis, even after 21 days of exposure. Bioelectromagnetics 24:465–472, 2003. © 2003 Wiley‐Liss, Inc.
Effect of a 125 mT static magnetic field on the kinetics of voltage activated Na+ channels in GH3 cells
Authors: Rosen Arthur D. 
Published: September 2003

AbstractVoltage activated Na+ channels were examined in GH3 cells, using the whole cell patch clamp method. Channel currents were recorded before, during, and after a 150 s exposure to a 125 mT static magnetic field. There was a slight shift in the current–voltage relationship and a less than 5% reduction in peak current during magnetic field exposure. More pronounced, however, was an increase in the activation time constant, τm, during and for at least 100 s following exposure to the field. This change in τm was seen primarily at lower activation voltages. No change was noted in the inactivation time constant, τh. Changes were clearly temperature dependent, being evident only at and above 35 °C. These findings are consistent with the hypothesis that reorientation of diamagnetic anisotropic molecules in the cell membrane are capable of distorting imbedded ion channels sufficiently to alter their function. The temperature dependence of this phenomenon is probably due to the greater ease with which a liquid crystal membrane can be deformed. Bioelectromagnetics 24:517–523, 2003. © 2003 Wiley‐Liss, Inc.
RF nonlinear interactions in living cells–II: Detection methods for spectral signatures
Authors: Balzano Quirino 
Published: September 2003

AbstractThe presence of harmonic products due to possible nonlinear interaction of amplitude modulated RF signals in living cells is best detected by using a cavity with high quality factor. Harmonic products generated by elementary oscillators can be trapped and accumulated in a cavity, permitting detection sensitivity much greater than in an open environment, where they would be radiated in all directions. The experimental method described herein is a systematic approach to detection of the non‐Planck RF energy (if any) emitted by an exposed sample of living cells. Balzano and Sheppard [Balzano and Sheppard (2003): Bioelectromagnetics 24:473–482] classified the non‐Planck RF emissions from living cells as coming from (1) nonlinear interactions and (2) inelastic interactions. Nonlinear harmonic products would appear in the band at twice the frequency of an amplitude modulated RF carrier. Inelastic interaction products resulting from the interaction between the incident RF energy and normally occurring mechanical vibrations are found in the band immediately adjacent to the carrier. Detection of the latter signals is difficult because of this close spectral proximity, for example, 1 part in 107 for 100 Hz modulation of a GHz carrier. Modern audio spectrum analyzers have excellent selectivity, providing 60 dB rejections only 2 kHz away from the carrier. By judicious selection of the amplitude modulation (AM) frequency, frequency of the RF carrier, and size of the biological sample, it is possible to achieve very high sensitivity (about −90 dBm) with commercially available instrumentation. The presence (or absence) of harmonics in the band adjacent to the amplitude modulated RF carrier would establish (or negate) the existence of coherent interactions between mechanical vibrations in the cell ensemble and the incident RF signal. Bioelectromagnetics 24:483–488, 2003. © 2003 Wiley‐Liss, Inc.