bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards Solid‐State Batteries Using a Calcium Hydridoborate Electrolyte
Authors: Skov Lasse N. , Grinderslev Jakob B. , Kjær Therese S. S. , Kristensen Lasse R. , Jensen Torben R. 
Published: January 2025

AbstractSolid‐state batteries created from abundant elements, such as calcium, may pave the way for cheaper and safer electrical energy storage. Here we report a new type of solid calcium hydridoborate electrolyte, Ca(BH4)2 ⋅ 2NH2CH3, with a high ionic conductivity of σ(Ca2+) ~10−5 S cm−1 at T=70 °C, which is assigned to a relatively open and flexible structure with apolar moieties and weak dihydrogen bonds that facilitate migration of Ca2+ ions in the solid state. The compound display a low electronic conductivity, providing an ionic transport number close to unity (tion=0.9916). Calcium plating is observed for a Ca|Ca(BH4)2 ⋅ 2NH2CH3|Pt electrochemical cell and the electrodes are investigated using scanning electron microscopy (SEM) combined with energy‐dispersive X‐ray spectroscopy (EDS) that reveal a rugged Ca anode surface owing to the stripping process and the presence of Ca‐containing domains on the Pt working electrode from the plating process. Improved electrochemical reversiblity was achieved in a three‐electrode cell configuration using a CaxSn counter and reference electrode and a Sn working electrode, CaxSn | Ca(BH4)2 ⋅ 2NH2CH3 | Sn, providing reversible Ca plating and stripping.
Manipulating Dynamic Light‐Driven Solid‐Liquid Transition and Static Reversible Photochromism by an Organic Cocrystal Strategy
Authors: Li Shuzhen , Huang Xueyong , Xing Menghao , Zhao Dongpu , Li Sitong , Cao Xiaoyu 
Published: January 2025

AbstractDeveloping of molecular crystalline materials with light‐induced multiple dynamic deformation in space dimension and photochromism on time scales has attracted much attention for its potential applications in actuators, sensoring and information storage. Nevertheless, organic crystals capable of both photoinduced dynamic effects and static color change are rare, particularly for multi‐component cocrystal system. In this study, we first report the construction of charge transfer cocrystal allows their light‐induced solid‐to‐liquid transition and photochromic behaviors to be controlled by trans‐stilbene (TSB) as an electron donor and 3,4,5,6‐tetrafluorophthalonitrile (TFP) as an electron acceptor. In this case, the dynamic photo‐responsive solid‐to‐liquid phase transition is due to the photoisomerization of TSB under UV light irradiation, while the accumulation of melted droplets during solid‐state photochemical process causes mechanical deformation of TSB‐TFP cocrystals. The subsequent reversible photochromic behavior is attributed to the emergence of free radicals through a photo‐induced electron transfer. Moreover, TSB‐TFP microcrystals present typical excitation wavelength dependent emission (EWDE) fluorescence by surfactant‐mediated method. This work realizes the dynamic‐static photochemical cascade processes in response to UV light irradiation in an organic cocrystal system, providing the effective method for a new type of smart photo‐responsive materials.
Ribose Sugar Alters Conformational Sampling of G⋅T Mismatched Duplex DNA
Authors: Jaisal Manjula , Sannapureddi Rajesh Kumar Reddy , Ash Subhaprad , Sathyamoorthy Bharathwaj 
Published: January 2025

AbstractPolymerases erroneously incorporate Guanine‐Thymine (dG⋅dT) mismatches in genomic DNA that further evades repair by transient sampling of tautomeric/ionic states compromising fidelity of repairing dG⋅dT mismatches. In conjunction, significant frequency of ribose (mis)incorporation in duplex DNA permits for misincorporated‐mismatch in the genome. Ribose incorporated G (rG) mismatched with T (rG⋅dT) is the most stable across all misincorporated‐mismatch calling into question the conformational consequences of the ribose sugar in addition to the mismatch. In this work, the effects of single rG⋅dT is investigated within a dodecamer DNA duplex employing solution‐state NMR spectroscopy, partial anisotropic measurements in conjunction with molecular dynamics simulations to evaluate the impact on base pairs and the overall duplex structure. It is observed that rG⋅dT pairs exhibit enhanced flexibility in both base‐pair and sugar dynamics compared to dG⋅dT, and the perturbations are enhanced in comparison to a ribose incorporated adenine‐thymine (rA−dT) pair. The structural perturbations compared between rG⋅dT and dG⋅dT provides clues on plausible recognition modes of ribonucleotide excision repair (RER) pathway that looks for misincorporated ribose and mismatch repair (MMR) enzymes that scout for a mismatch.
Investigating the impact of synonymous gene recoding on a recombinantly expressed monoclonal antibody under different process parameters
Authors: Kaissarian Nayiri M. , Sandefur Stephanie L. , Ghosh Arnab , Katneni Upendra K. , Walton Wendy , Frye Christopher C. , Komar Anton A. , Kimchi‐Sarfaty Chava 
Published: January 2025

AbstractMonoclonal antibodies (mAbs) are commonly used biologic therapeutics with a wide variety of clinical applications. During the development process, manufacturers consider different production parameters to improve protein yield and achieve appropriate quality of the product. Synonymous gene recoding is one of such attributes that is often considered and implemented to enhance protein expression. However, it has to be used with caution, as it may lead to protein misfolding and ER stress, which complicates efforts to manufacture the desired mAb. To investigate how changing mRNA sequence composition under different protein production parameters might affect the quality of recombinantly produced mAbs, we performed a comprehensive and systematic study assessing impact of synonymous gene recoding (commonly referred to as codon optimization) strategies in the context of varied cell culture parameters on product quality, biochemical and functional characteristics. We report the impact of these parameters on mAb glycosylation profiles, charge variant profile, aggregation, fragmentation, and mAb functional response from combinations of different production parameters. These results uncovered a complex interplay of sequence composition and manufacturing parameters and emphasize the importance of assessing changes to key quality attributes when optimizing mAb manufacturing, including the use of synonymous gene recoding.
Conducting a Co‐Operative Inquiry in the Field of Counselling: Critical Reflections and Learnings From a ‘First‐Time’ Inquirer
Authors: McGarry Amanda 
Published: January 2025

ABSTRACTIntroductionCo‐operative inquiry, a form of participatory action research, has been utilised as a method within the counselling field for some time, and whilst reflections have been shared about the use of this outside of the field, little commentary has been offered from within it. This paper outlines critical reflections and learnings from conducting a co‐operative inquiry as a ‘first time inquirer’ to provide insight for other ‘first timers’, and those conducting this method within the counselling field.MethodNine co‐researchers met on 11 occasions from March to October 2022, exploring various aspects in relation to the research topic. Thoughts and experiences were communicated in a variety of ways, through poetry, storytelling, and journaling. The researcher kept a reflexive journal throughout the co‐operative inquiry process, noting the importance of this process to participatory research.Findings and DiscussionThe reflections highlighted are based around key areas of the inquiry, such as how the group set off, the nature of meetings, and how the data analysis process was managed. Various points of discussion are highlighted such as the use of a working agreement, power dynamics, and the impact of the day job. In addition, this paper outlines a pragmatic approach to co‐operative inquiry suitable for doctoral level research.
Synthesis, Biological Activities, DFT Calculations, and Molecular Docking Studies of O‐Methyl‐Inositols
Authors: Aksu Kadir , Ayvaz Melek Çol , Çelik Ömer Faruk , Serdaroğlu Goncagül , Üstün Elvan , Kelebekli Latif 
Published: January 2025

ABSTRACTThe concise synthesis of O‐methyl‐d‐inositol derivative and conduritol derivatives was obtained starting from p‐benzoquinone. Spectroscopic methods have been performed for the characterization of newly synthesized compounds. Cyclitols are useful molecules with anticancer, antibiotic, antinutrient, and antileukemic activities. Inositol class molecules, known as the most important cyclitol derivatives, were examined in this study for their 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and nitric oxide radical scavenging and butyrylcholinesterase (BChE) and glycosidase inhibition activities. It was observed that compound 5, in particular, showed efficacy that competed with the standards in terms of both antioxidant activity and enzyme inhibitor potential. Additionally, compound 5 shows effective antimicrobial activity. The water‐soluble characteristics and lipophilic properties of the compounds were also considered and discussed. Moreover, the quantum chemical analyses were performed in light of the DFT/B3LYP/6‐311G** level computations to elucidate/compare the studied inositols’ possible reactivity directions. Additionally, the interactions of the molecules were analyzed against acetylcholinesterase (AChE), peroxiredoxin 5, and DNA gyrase by molecular docking methods. Cholinesterase inhibitors have an important status as the most important drug group used in the treatment of Alzheimer's disease today. Considering the effects of inhibition of the α‐glucosidase enzyme by inhibitors, such molecules can also be used as therapeutic components in the treatment of diabetes.
A Review of Bavachinin and Its Derivatives as Multi‐Therapeutic Agents
Authors: Zeng Jun Lin , Lan Jin Xia , Dai Wei , Liu Sheng Lan , Huang Hao , Shu Guang Zhao , Huang Le Jun , Kang Si Shuang , Chen Bin , Hou Wen 
Published: January 2025

ABSTRACTExtracting natural active ingredients from plants is an effective way to develop and screen modern drugs. Psoralea corylifolia (PC) is a leguminous plant whose seeds have long been used as a Traditional Chinese Medicine to treat psoriasis, rheumatism, dermatitis, and other diseases. To date, several main compounds, including coumarins, flavonoids, monoterpene phenols, and benzofurans, have been identified from the seeds of PC. Among them, bavachinin is a type of flavonoid with various biological activities. In this article, the biological activities and mechanisms of action of bavachinin and its derivatives are reviewed. It includes the pharmacokinetic characteristics of bavachinin and its derivatives, as well as its prominent anti‐inflammatory, antitumor, antibacterial, and antiviral pharmacological activities and related metabolic studies. Bavachinin displayed these activities through different receptors, such as peroxisome proliferator‐activated receptors (PPARs), as well as multiple signaling pathways and enzyme systems. In summary, bavachinin and its derivatives have potential drug development value in many fields, such as anti‐inflammatory, antitumor, nervous system disease, and diabetes. We believe that this review will lay a foundation for bavachinin‐based drug development throughout the world.
Delving Into lncRNA‐Mediated Regulation of Autophagy‐Associated Signaling Pathways in the Context of Breast Cancer
Authors: Hsu Chou‐Yi , Jasim Saade Abdalkareem , Bansal Pooja , Kaur Harpreet , Ahmad Irfan , Saud Abdulnaser , Deorari Mahamedha , Al‐Mashhadani Zuhair I. , Kumar Abhinav , Zwamel Ahmed Hussein 
Published: January 2025

ABSTRACTBreast cancer is a multifaceted and prevalent malignancy, impacting a considerable proportion of women globally. Numerous signaling pathways intricately regulate cellular functions such as growth, proliferation, and survival. Among the various regulators, lncRNAs have emerged as significant players despite their inability to encode proteins. An expanding body of literature underscores the pivotal roles lncRNAs play in cancer biology, particularly in the context of breast cancer. Autophagy, the cellular process dedicated to the degradation and recycling of cellular components, is now recognized as a crucial factor in cancer initiation and progression. The interplay between lncRNAs, various signaling pathways, and autophagy in the pathophysiology of breast cancer remains an active area of investigation. Researchers have identified specific lncRNAs that are dysregulated in breast cancer patients, influencing the modulation of key signaling pathways. Using experimental methodologies and bioinformatics approaches, multiple lncRNAs have been elucidated, providing deeper insights into their contributions to breast cancer pathogenesis and metastatic processes. In summary, the pathophysiological landscape of breast cancer is characterized by the complex interactions involving lncRNA‐mediated autophagy. This understanding paves the way for identifying novel therapeutic targets, prognostic markers, and diagnostic markers, ultimately contributing to improved treatment outcomes in breast cancer management.
Improving the Nutritional Quality of Instant Noodles Made From Wheat Flour Using Dry and Wet Fractionated Mungbean Protein Ingredients
Authors: Skylas Daniel J. , Whiteway Chris , Webster Theo , Messina Valeria , Richard Sonya , Siah Siem Doo , Quail Ken J. 
Published: January 2025

ABSTRACTBackground and ObjectivesThe nutritional quality of instant noodles can be improved using dry and wet fractionated mungbean protein ingredients. Wheat flour was blended with mungbean protein concentrate and protein isolate to produce high‐protein instant noodles of around 15%, 20%, and 25% protein. The effects on nutritional composition, protein quality, texture, cooking, and color properties of instant noodles were investigated.FindingsSignificant differences (p < 0.05) were observed in the nutritional, physico‐chemical, and functional properties of wheat flour and mungbean protein ingredients. Water and oil holding capacities were higher for mungbean protein isolate and protein concentrate compared to mungbean and wheat flour ingredients. Blends of wheat flour with mungbean protein concentrate and protein isolate produced instant noodles with significantly higher (p < 0.05) protein and mineral content, and lower fat content, compared to the wheat flour control. The protein content of instant noodles ranged from 9.1 to 23.6 g/100 g (db.). Linear regression analysis showed a strong relationship between protein content and noodle firmness (R = 0.965). There was a significant increase (p < 0.05) in lysine content (1.7–11.5 mg/g flour) with the addition of protein concentrate and isolate, improving the amino acid score of lysine from 0.34 to 0.91. The color properties of fried instant noodles showed increased redness (a*) and yellowness (b*), and lightness (L*) decreased with increasing protein content. There were no significant differences (p > 0.05) in cooking losses but there were significant differences (p < 0.05) in water uptake, being lower for instant noodles blended with protein isolate.ConclusionsInstant noodles blended with wheat flour and mungbean protein concentrate and isolate had higher protein content, with improved nutritional composition and protein quality. Instant noodles blended with protein concentrate had significantly lower (p < 0.05) levels of fat and sodium content, and higher levels of calcium, magnesium, phosphorus, potassium, and zinc, compared to those blended with protein isolate. Instant noodles with improved nutritional composition have the potential to deliver increased health benefits to consumers.Significance and NoveltyThe key findings from this study will inform food manufacturers of the health benefits of mungbean and encourage the use of dry fractionated mungbean protein concentrate ingredients in popular and widely consumed food products.
Atrial Tachyarrhythmia in Arrhythmogenic Right Ventricular Cardiomyopathy: A Case Report
Authors: Sharma Shriya , Giri Ganesh , Khadka Sadiksha , Goswami Rohan 
Published: January 2025

ABSTRACTArrhythmogenic right ventricular cardiomyopathy (ARVC) is a heritable myocardial condition that mostly affects the right ventricle (RV). Atrial involvement is poorly understood and the evidence for atrial involvement remains limited. In this case report, we describe an 18‐year‐old woman who had ARVC with the atrial tachyarrhythmia and initially presented with palpitations. As a rare disorder, ARVC requires a careful diagnosis to prevent unnecessary delays in patient care. In our case, the definitive diagnosis of ARVC was made as per Revised International Task Force Criteria 2010.
Well‐Controlled Block‐Type Cyclic Olefin Copolymers with High Heat Resistance, Outstanding Strength and Low‐Temperature Toughness
Authors: Huang Dong , Li Jin , Lu Xu , Zhang Kunyu , Wang Fei , Wu Di , Wang Yafei , Pan Li , Li Yang , Li Yuesheng 
Published: January 2025

Comprehensive SummaryAs an important class of transparent polyolefins, cyclic olefin copolymers (COCs) exhibit a wide range of properties, from soft (low cyclic unit content in a random copolymer) to hard (high cyclic unit content in a random copolymer) thermoplastics. A higher cyclic unit content in a random copolymer results in increased rigidity and heat resistance, but at the expense of material toughness, especially under low temperature. To achieve a trade‐off, block‐type COCs composed of well‐defined hard‐soft and hard‐soft‐hard segments with high molecular weights (Mn > 200 kDa) and relatively narrow molecular weight distributions (Mw/Mn < 1.42) were prepared via quasi‐living copolymerization. Compared to random copolymers, di‐block COCs retained satisfactory heat resistance and optical transparency and exhibited superior tensile properties (tensile strength: 56.2 MPa, elongation at break: 254.4%), while tri‐block COCs with the same hard‐soft segments and an additional hard segment exhibited even better tensile properties, achieving a tensile strength of 67.3 MPa, elongation at break of 286.9%, all while maintaining high optical transmittance (> 90%). Even at temperatures below 0 °C, the tri‐block copolymers EN52‐b‐EN19‐b‐EN46 demonstrated a satisfactory tensile strength (> 64.0 MPa) and elongation at break (> 65.6%), highlighting the advantages of the alternating hard‐soft segment structural design in COCs.
Incidence and Predictors of Clinical Outcomes in Real‐Life Patients With Atrial Fibrillation Treated With Oral Factor Xa Inhibitors: The Follow‐Up Results of the ANATOLIA‐AF Study
Authors: Kocabaş Umut , Ergin Isil , Sönmez Sadi Can , Yavuz Veysel , Murat Selda , Özdemir Ibrahim Halil , Genç Ömer , Tüner Haşim , Meriç Bengisu Keskin , Aslan Onur , Dal Ahmet , Taşkın Uğur , Şen Taner , İbişoğlu Ersin , Erdoğan Aslan , Özgeyik Mehmet , Demir Mevlüt , Urgun Örsan Deniz , Doğduş Mustafa , Çakal Sinem , Çayırlı Sercan , Güler Arda , Karabulut Dilay , Dalgıç Onur , Murat Bektaş , Karabulut Umut , Öztekin Gülsüm Meral Yılmaz , Biter Halil İbrahim , Sinan Ümit Yaşar , Barış Veysel Özgür , Kaplan Mehmet , Altın Cihan , Kıvrak Tarık 
Published: January 2025

ABSTRACTObjectiveThe main objective of this study is to determine the incidence and predictors of clinical outcomes in patients with AF treated with factor Xa inhibitors in a real‐world setting.MethodsThe present study was a multicentre and observational study that included patients with AF who were treated with factor Xa inhibitors. The primary outcome was the composite of ischemic stroke, TIA, systemic embolism, major bleeding, and all‐cause mortality.ResultsA total of 1162 patients from 26 cardiology centers were included in this study, with a median age of 72 years. During the median 12‐month follow‐up period, the primary outcome occurred in 195 patients (16.8%). Treatment with rivaroxaban compared with apixaban and edoxaban showed a lower rate of ischemic stroke, TIA, and/or systemic embolism (2.2% vs. 4.7% vs. 6.5%, respectively, p = 0.014). The major bleeding rate was similar between all three factor Xa inhibitors. The all‐cause mortality rate in the rivaroxaban group was lower compared with the apixaban and edoxaban groups (9.8% vs. 15.1% vs. 12.4%, respectively, p = 0.042). Overall, the frequency of primary outcome was 13.8%, 19.6%, and 20.6% for patients treated with rivaroxaban, apixaban, and edoxaban, respectively (p = 0.019). Older age, male sex, low body weight, high bleeding risk, heart failure, hypertension, liver failure, and treatment with apixaban 2.5 mg b.i.d. were independently associated with the development of primary outcome.ConclusionThe follow‐up data from the ANATOLIA‐AF study provides detailed data about the incidence and independent predictors of adverse clinical outcomes in patients with AF treated with factor Xa inhibitor treatment.
Analyzing Pathophysiology and Immune Cells and Their Cytokines and Mediators in Precision‐Cut Slices of the Murine Lung
Authors: Tatcheff Michaela , Carvalho Christine , Willar Jonas , Nendel Elvedina , Krammer Susanne , Chiriac Mircea T. , Zhou Shuting , Geppert Carol I. , Finotto Susetta 
Published: January 2025

AbstractUnderstanding the dynamic pathophysiology of diseases in the lung, such as asthma and chronic asthma, chronic obstructive pulmonary disease, and lung cancer, is crucial for the treatment, analysis, and outcome of these diseases. Unlike other traditional models, we suggest a protocol that is sustainable and reproducible and offers different analysis methods while maintaining in vivo lung architecture and immune dynamics. This protocol allows one to study the pathophysiological changes, including changes to the immune cells, cytokines, and mediators, in 30 precision‐cut lung slices from a single murine lung. To accomplish this, the murine lung is infused with 2.5% low‐melting‐point agarose and is precision‐cut‐sliced. Our method also supports cell culture in refined medium and stimulation with clinically relevant stimuli, which helps to clarify the mechanisms of the disease. Evaluation of the samples and their supernatant includes multiplex assays, ELISA, histology, and immunohistochemistry. Additional sections are used to extract RNA for quantitative real‐time PCR and RNA sequencing and/or other selected analysis, like flow cytometry. Using this method, we obtained murine lung slices that preserve the pathophysiology of the disease and allow a comprehensive analysis unlike other already‐existing protocols. By retaining the dynamic immune mechanisms, we are able to see the histological damage caused by each disease. The results of this protocol can be used to improve our understanding and therapy options. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Obtaining precision‐cut lung slices (PCLSs) from the murine lungSupport Protocol 1: LL/2 cell treatment with G‐418 solutionSupport Protocol 2: Murine model of lung adenocarcinoma and in vivo imagingSupport Protocol 3: H&E staining of PCLS sectionsSupport Protocol 4: Measuring cytokines by ELISASupport Protocol 5: Measuring cytotoxic activity in PCLS conditioned mediumSupport Protocol 6: Analysis of RNA from PCLSs by real‐time PCRSupport Protocol 7: Flow cytometry analysis of cells isolated from PCLSs