bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rotational Thromboelastometry and Clot Waveform Analysis as Point-of-Care Tests for Diagnosis of Disseminated Intravascular Coagulation in Critically Ill Children in Thailand*
Authors: Chanthong Supapitch , Choed-Amphai Chane , Manowong Suphara , Tuntivate Pakinee , Tansriratanawong Supakanya , Makonkawkeyoon Krit , Natesirinilkul Rungrote 
Published: February 2024

OBJECTIVES:This study aimed to determine the test performances of rotational thromboelastometry (ROTEM) and activated partial thromboplastin time-based clot waveform analysis (aPTT-CWA) compared with the International Society on Thrombosis and Hemostasis disseminated intravascular coagulation (ISTH-DIC) score for diagnosis of overt disseminated intravascular coagulation (ODIC) in critically ill children. Prognostic indicators of DIC complications were also evaluated.DESIGN:A prospective cross-sectional observational study was conducted. ROTEM and aPTT-CWA were assessed alongside standard parameters based on the ISTH-DIC score and natural anticoagulants. Both conventional and global hemostatic tests were repeated on days 3–5 for nonovert DIC.SETTING:PICU of the Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.SUBJECTS:Infants and children who were admitted to PICU with underlying diseases predisposed to DIC, such as sepsis, malignancy, major surgery, trauma, or severe illness, were included in the study between July 1, 2021, and November 30, 2022.INTERVENTIONS:None.MEASUREMENTS AND MAIN RESULTS:Sixty-four children were enrolled in this study. The prevalence of ODIC was 20.3%. Regarding ROTEM parameters, using EXTEM clot formation time (CFT) cutoff of greater than 102 seconds provided sensitivity and specificity of 90.9% and 80.9%, respectively, for diagnosing ODIC, with the area under the curve (AUC) of 0.86. In the case of aPTT-CWA performance, no biphasic waveform was observed, whereas both maximum coagulation acceleration (Min2) of less than 0.35%/s2and maximum coagulation deceleration of less than 0.25%/s2demonstrated identical sensitivities of 76.9% and specificities of 79.6%. Combining two global hemostatic tests significantly improved the diagnostic performance (INTEM CFT + EXTEM CFT + Min2 AUC 0.92 [95% CI, 0.80–1.00] vs. EXTEM CFT AUC 0.86 [95% CI, 0.75–0.96],p= 0.034). Bleeding was the most common consequence. In multivariable logistic regression analysis, Min2 of less than 0.36%/s2was an independent risk factor for bleeding complications, with an adjusted odds ratio of 15.08 (95% CI, 1.08–211.15,p= 0.044).CONCLUSIONS:ROTEM and aPTT-CWA were valuable diagnostic tools in critically ill children who might require point-of-care tests. Min2 showed significant clinical implications for predicting bleeding events in this population.
High Variability in the Duration of Chest Compression Interruption is Associated With Poor Outcomes in Pediatric Extracorporeal Cardiopulmonary Resuscitation
Authors: Han Peggy , Rasmussen Lindsey , Su Felice , Dacre Michael , Knight Lynda , Berg Marc , Tawfik Daniel , Haileselassie Bereketeab 
Published: February 2024

OBJECTIVES:To determine the association between chest compression interruption (CCI) patterns and outcomes in pediatric patients undergoing extracorporeal cardiopulmonary resuscitation (ECPR).DESIGN:Cardiopulmonary resuscitation (CPR) data were collected using defibrillator-electrode and bedside monitor waveforms from pediatric ECPR cases between 2013 and 2021. Duration and variability of CCI during cannulation for ECPR was determined and compared with survival to discharge using Fishers exact test and logistic regressions with cluster-robustses for adjusted analyses.SETTING:Quaternary care children’s hospital.PATIENTS:Pediatric patients undergoing ECPR.INTERVENTIONS:None.MEASUREMENTS AND MAIN RESULTS:Of 41 ECPR events, median age was 0.7 years (Q1, Q3: 0.1, 5.4), 37% (15/41) survived to hospital discharge with 73% (11/15) of survivors having a favorable neurologic outcome. Median duration of CPR from start of ECPR cannulation procedure to initiation of extracorporeal membrane oxygenation (ECMO) flow was 21 minutes (18, 30). Median duration of no-flow times associated with CCI during ECMO cannulation was 11 seconds (5, 28). Following planned adjustment for known confounders, survival to discharge was inversely associated with maximum duration of CCI (odds ratio [OR] 0.91 [0.86–0.95],p= 0.04) as well as the variability in the CCI duration (OR 0.96 [0.93–0.99],p= 0.04). Cases with both above-average CCI duration and higher CCI variability (sd> 30 s) were associated with lowest survival (12% vs. 54%,p= 0.009). Interaction modeling suggests that lower variability in CCI is associated with improved survival, especially in cases where average CCI durations are higher.CONCLUSIONS:Shorter duration of CCI and lower variability in CCI during cannulation for ECPR were associated with survival following refractory pediatric cardiac arrest.
An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangioma
Authors: Holm Annegret , Graus Matthew S. , Wylie-Sears Jill , Borgelt Luke , Heng Tan Jerry Wei , Nasim Sana , Chung Long , Jain Ashish , Sun Mingwei , Sun Liang , Brouillard Pascal , Lekwuttikarn Ramrada , Kozakewich Harry , Qi Jacob Yanfei , Teng Joyce C. , Mulliken John B. , Vikkula Miikka , Francois Mathias , Bischoff Joyce 
Published: February 2024

ABSTRACTInfantile hemangioma (IH) is the most common tumor in children and a paradigm for pathological vasculogenesis, angiogenesis and regression. Propranolol is the mainstay of treatment for IH. It inhibits hemangioma vessel formation via a β-adrenergic receptor independent off-target effect of its R(+) enantiomer on the endothelial specific transcription factor sex-determining region Y (SRY) box transcription factor 18 (SOX18). Transcriptomic profiling of patient-derived hemangioma stem cells uncovered the mevalonate pathway (MVP) as a target of R(+) propranolol. Loss of SOX18 function confirmed R(+) propranolol mode of action on the MVP. Functional validation in preclinical IH models revealed that statins - targeting the MVP - are potent inhibitors of hemangioma vessel formation. We propose a novel SOX18-MVP-axis as a central regulator of IH pathogenesis and suggest statin repurposing to treat IH. Our findings reveal novel pleiotropic effects of beta-blockers and statins acting on the SOX18-MVP axis to disable an endothelial specific program in IH, which may impact other scenarios involving pathological vasculogenesis and angiogenesis.Graphical abstract
A novel microporous biomaterial vaccine platform for long-lasting antibody mediated immunity against viral infection
Authors: Mayer Daniel P. , Neslon Mariah E. , Andriyanova Daria , Antao Olivia Q. , Chen Jennifer S. , Scumpia Philip O. , Weaver Westbrook M. , Deshayes Stephanie , Weinstein Jason S. 
Published: February 2024

ABSTRACTCurrent antigen delivery platforms, such as alum and nanoparticles, are not readily tunable, thus may not generate optimal adaptive immune responses. We created an antigen delivery platform by loading lyophilized Microporous Annealed Particle (MAP) with aqueous solution containing target antigens. Upon administration of antigen loaded MAP (VaxMAP), the biomaterial reconstitution forms an instant antigen-loaded porous scaffold area with a sustained release profile to maximize humoral immunity. VaxMAP induced CD4+ T follicular helper (Tfh) cells and germinal center (GC) B cell responses in the lymph nodes similar to Alum. VaxMAP loaded with SARS-CoV-2 spike protein improved the magnitude and duration of anti-receptor binding domain antibodies compared to Alum and mRNA-vaccinated mice. A single injection of Influenza specific HA1-loaded-VaxMAP enhanced neutralizing antibodies and elicited greater protection against influenza virus challenge than HA1-loaded-Alum. Thus, VaxMAP is a platform that can be used to promote adaptive immune cell responses to generate more robust neutralizing antibodies, and better protection upon pathogen challenge.
Combination therapies delay cognitive decline over 10 years in Alzheimer’s NACC participants
Authors: Shang Yuan , Torrandell-Haro Georgina , Vitali Francesca , Brinton Roberta Diaz 
Published: February 2024

AbstractINTRODUCTIONDelaying cognitive decline in Alzheimer’s disease can significantly impact both function and quality of life.METHODSLongitudinal analysis of National Alzheimer’s Coordinating Center (NACC) dataset of 7,653 mild dementia CDR-SB AD participants at baseline with prescriptions for diabetes (DBMD), lipid-lowering (LIPL), anti-hypertensive (AHTN), and non-steroidal anti-inflammatory (NSD) medications over 10 years was evaluated for change in cognitive function relative to non-treated stratified by sex and APOE genotype.RESULTSCombination therapy of DBMD+LIPL+AHTN+NSD resulted in a 44% / 35% (MMSE/CDR-SB) delay in cognitive decline at 5 years and 47% / 35% (MMSE/CDR-SB) delay at 10 years. Females and APOE4 carriers exhibited greatest cognitive benefit of combination therapy.DISCUSSIONCombination therapies significantly delayed cognitive decline in NACC AD participants at a magnitude comparable to or greater than beta-amyloid immunomodulator interventions. These data support combination precision medicine targeting AD risk factors to alter the course of the disease that persists for a decade.
Prevalence of ocular manifestations in cutaneous rosacea: Protocol for a systematic review and meta-analysis
Authors: Nazzicone Kristina , Kirkpatrick Ryan H. , Biorac Aleksandar , Froentjes Anneke R. , Molin Sonja , Simpson Sarah 
Published: February 2024

ABSTRACTIntroduction: Rosacea is a chronic inflammatory skin condition with concomitant ocular manifestations and neurogenic symptoms. Ocular rosacea poses a particular diagnostic challenge as its signs and symptoms often overlap with other ocular pathologies. Cutaneous and ocular rosacea present together in approximately 21-50% of patients, yet a solid understanding of comorbid ocular and cutaneous symptoms is lacking. Therefore, the present paper outlines a protocol for a systematic review and meta-analysis to describe and quantify the prevalence of ocular rosacea in cutaneous rosacea and each of its subtypes. Methods: This study will follow Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and be conducted using the systematic review software, Covidence. To determine inclusion, screening will occur at two levels (title and abstract-only followed by full-text) and will be completed separately by two authors. Primary research studies on ocular rosacea in adult cutaneous rosacea published in all languages and geographic regions until November 2023 will be reviewed for inclusion. Data pertaining to overall prevalence of ocular rosacea across and within cutaneous rosacea subtypes, mean age, sex, gender, ethnicity, socioeconomic status, time to diagnosis, time to treatment, and prevalence of comorbid conditions will be extracted. For each included study, the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) framework will be applied to assess study quality. Conclusion: To the authors’ knowledge, this will be the first systematic review and meta-analysis comparing the prevalence of ocular rosacea in the various cutaneous subtypes in an adult population. By addressing this knowledge gap, this study aims to provide clear and easily interpretable data to aid in the early diagnosis and treatment of ocular rosacea. This study is registered in the International Prospective Register of Systematic Reviews (PROSPERO ID# CRD42023475026).Key MessageProtocol for a systematic review and meta-analysis investigating the prevalence of ocular manifestations in adult rosacea
Association between lifestyle at different life periods and brain integrity in older adults
Authors: Turpin Anne-Laure , Felisatti Francesca , Mézenge Florence , Landeau Brigitte , Vivien Denis , de la Sayette Vincent , Chételat Gaël , Gonneaud Julie ,   the Medit-Ageing Research Group
Published: February 2024

AbstractImportanceLifestyle factors have been associated with dementia risk and neuroimaging markers of ageing and Alzheimer’s Disease (AD), but the period at which they have the greatest influence remains unclear.ObjectiveTo determine the relative influence of lifestyle at different life periods on older adults’ brain health.Design, Setting, and ParticipantsBaseline data from the Age-Well trial were used in this study. Cognitively unimpaired participants aged 65 years and older were recruited in the general population between November 2016 to March 2018 in Caen, France. Analysis took place between June 2022 and September 2023.Exposure(s)The Lifetime of Experiences Questionnaire (LEQ) was used to assess lifestyle during young adulthood (3-30y), midlife (30-65y) and late-life (>65y). For each life period, LEQ score is divided into specific and non-specific subscores.Main Outcome(s) and Measure(s)Multiple regressions were conducted including lifestyle at the three life periods (in the same model) to predict gray matter volume (GMv; from structural MRI), glucose metabolism (FDG-PET), perfusion (Florbetapir-PET, early-acquisition) and amyloid burden (Florbetapir-PET, late-acquisition), both in AD-sensitive regions and voxel-wise, controlling for age and sex. Then, comparisons of correlations between lifestyle at each life period, as well as between specific versus non-specific activities, and neuroimaging outcomes were performed.ResultsOf the 135 older adults (mean age=69.3±3.79), 61.5% were women. No significant association was found between LEQ scores and AD-sensitive regions. While LEQ-young was not associated with neuroimaging, LEQ-midlife was more strongly associated with GMv, including in the anterior cingulate cortex, and with amyloid burden in the precuneus compared to the other periods. LEQ-late showed stronger associations with perfusion and glucose metabolism than LEQ-young and LEQ-midlife in medial frontal regions. Lower amyloid burden was more strongly correlated with LEQ-midlife specific than non-specific activities (z=-2.0977, p<.05, [95% CI, −0.3985 - −0.0102]) while perfusion was more strongly correlated with LEQ-late non-specific than specific scores (z=2.4369, p<.01, [95% CI, 0.0.415-0.4165]).Conclusions and RelevanceLifestyle at different life periods might have complementary benefits on structural/molecularversusfunctional markers of brain health in late-life. Interestingly, these associations were found in regions related to reserve/resilience and aging.Trial registrationClinicaltrials.govIdentifier:NCT02977819
Receptor kinase LecRK-I.9 regulates cell wall remodelling and signalling during lateral root formation inArabidopsis
Authors: Bellande Kevin , Roujol David , Chourré Josiane , Gall Sophie Le , Martinez Yves , Jauneau Alain , Burlat Vincent , Jamet Elisabeth , Canut Hervé 
Published: February 2024

AbstractAssembling and remodelling the cell wall is essential for plant development. Cell wall dynamic is controlled by cell wall proteins and a variety of sensor and receptor systems. LecRK-I.9, anArabidopsis thalianaplasma membrane-localised lectin receptor kinase, was previously shown to be involved in cell wall-plasma membrane contacts and to play roles in plant-pathogen interactions, but so far, its role in development was unknown.LecRK-I.9is transcribed at a high level in root tissues including the pericycle. Comparative transcript profiling of a loss-of-function mutantvswild type identifies LecRK-I.9 as a regulator of cell wall metabolism. Consistently,lecrk-I.9mutants display an increased pectin methylesterification level correlated with decreased pectin methylesterase and increased polygalacturonase activities. Also, LecRK-I.9 impacts lateral root development through the regulation of genes encoding (i) cell wall remodelling proteins during early events of lateral root initiation, and (ii) cell wall signalling peptides (CLE2, CLE4) repressing lateral root emergence and growth. Besides, low nitrate reducesLecRK-I.9expression in pericycle and interferes with its regulatory network: however, the control ofCLE2andCLE4expression is maintained. Altogether, the results show that LecRK-I.9 is a key player in a signalling network regulating both pre-branch site formation and lateral root emergence.HighlightThe lectin receptor kinase LecRK-I.9 regulates the molecular events leading to lateral root formation in both the initiation and emergence processes in Arabidopsis through cell wall remodelling enzymes and signalling peptides.
Integrative analysis of nanopore direct RNA sequencing data reveals a role of PUS7-dependent pseudouridylation in regulation of m6A and m5C modifications
Authors: Bansal Mohit , Kundu Anirban , Gupta Anamika , Ding Jane , Gibson Andrew , RudraRaju Sanjay Varma , Sudarshan Sunil , Ding Han-Fei 
Published: February 2024

AbstractUnderstanding the interactions between different RNA modifications is essential for unraveling their biological functions. Here, we report NanoPsiPy, a computational pipeline that employs nanopore direct RNA sequencing to identify pseudouridine (Ψ) sites and quantify their levels at single-nucleotide resolution. We validated NanoPsiPy by transcriptome-wide profiling of PUS7-dependent Ψ sites in poly-A RNA and rRNA. NanoPsiPy leverages Ψ-induced U-to-C basecalling errors in nanopore sequencing data, allowing detection of both low and high stoichiometric Ψ sites. We identified 8,624 PUS7-dependent Ψ sites in 1,246 mRNAs encoding proteins associated with ribosome biogenesis, translation, and energy metabolism. Importantly, integrative analysis revealed that PUS7 knockdown increases global mRNA N6-methyladenosine (m6A) and 5-methylcytosine (m5C) levels, suggesting an antagonistic relationship between Ψ and these modifications. Our study underscores the potential of nanopore direct RNA sequencing in revealing the co-regulation of RNA modifications and the capacity of NanoPsiPy in analyzing pseudouridylation and its impact on other RNA modifications.
Quark Mass Dependence of Heavy Quark Diffusion Coefficient from Lattice QCD
Authors: Altenkort Luis , de la Cruz David , Kaczmarek Olaf , Larsen Rasmus , Moore Guy D. , Mukherjee Swagato , Petreczky Peter , Shu Hai-Tao , Stendebach Simon ,   HotQCD Collaboration
Published: February 2024

We present the first study of the quark mass dependence of the heavy quark momentum and spatial diffusion coefficients using lattice QCD with light dynamical quarks corresponding to a pion mass of 320 MeV. We find that, for the temperature range 195  MeV<T<293  MeV, the spatial diffusion coefficients of the charm and bottom quarks are smaller than those obtained in phenomenological models that describe the pT spectra and elliptic flow of open heavy flavor hadrons.

Published by the American Physical Society
2024
The effect of classroom acoustics and noise on high school students’ listening, learning and well-being: a scoping review
Authors: Mealings Kiri , Buchholz Joerg M. 
Published: February 2024

Purpose
The purpose of this paper is to systematically map research on the effect of classroom acoustics and noise on high school students’ listening, learning and well-being, as well as identify knowledge gaps to inform future research.

Design/methodology/approach
This scoping review followed the PRISMA-ScR protocol. A comprehensive search of four online databases (ERIC, PubMed, Scopus and Web of Science) was conducted. Peer-reviewed papers were included if they conducted a study on the effect of classroom acoustics or noise on students’ listening, learning or well-being; had a clear definition of the noise level measurement; were conducted with high school students; and had the full text in English available.

Findings
In total, 14 papers met the criteria to be included in the review. The majority of studies assessed the impact of noise on students’ listening, learning or well-being. Overall, the results showed that higher noise levels have a negative effect on students’ listening, learning and well-being. Effects were even more pronounced for students who were non-native speakers or those with special educational needs such as hearing loss. Therefore, it would be beneficial to limit unnecessary noise in the classroom as much as possible through acoustic insulation, acoustic treatment and classroom management strategies.

Originality/value
This paper is the first review paper to synthesize previous research on the effect of classroom acoustics and noise on high school students’ listening, learning and well-being. It provides an analysis of the limitations of existing literature and proposes future research to help fill in these gaps.
An evaluation of 2013 privatization on Benin Electricity Distribution technical and workforce performance
Authors: Esan Oluwadamilola , Nwulu Nnamdi I. , David Love Opeyemi , Adepoju Omoseni 
Published: February 2024

Purpose
This study aims to investigate the impact of the 2013 privatization of Nigeria’s energy sector on the technical performance of the Benin Electricity Distribution Company (BEDC) and its workforce.

Design/methodology/approach
This study used a questionnaire-based approach, and 196 participants were randomly selected. Analytical tools included standard deviation, Spearman rank correlation and regression analysis.

Findings
Before privatization, the energy sector, managed by the power holding company of Nigeria, suffered from inefficiencies in fault detection, response and billing. However, privatization improved resource utilization, replaced outdated transformers and increased operational efficiency. However, in spite of these improvements, BEDC faces challenges, including unstable voltage generation and inadequate staff welfare. This study also highlighted a lack of experience among the trained workforce in emerging electricity technologies such as the smart grid.

Research limitations/implications
This study’s focus on BEDC may limit its generalizability to other energy companies. It does not delve into energy sector privatization’s broader economic and policy implications.

Practical implications
The positive outcomes of privatization, such as improved resource utilization and infrastructure investment, emphasize the potential benefits of private ownership and management. However, voltage generation stability and staff welfare challenges call for targeted interventions. Recommendations include investing in voltage generation enhancement, smart grid infrastructure and implementing measures to enhance employee well-being through benefit plans.

Social implications
Energy sector enhancements hold positive social implications, uplifting living standards and bolstering electricity access for households and businesses.

Originality/value
This study contributes unique insights into privatization’s effects on BEDC, offering perspectives on preprivatization challenges and advancements. Practical recommendations aid BEDC and policymakers in boosting electricity distribution firms’ performance within the privatization context.