bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural differences between non-lucid dreams, lucid dreams and out-of-body experience reports assessed by graph analysis
Authors: Gallo Francisco T. , Spiousas Ignacio , Herrero Nerea L. , Godoy Daniela , Tommasel Antonela , Gasca-Rolin Miguel , Ramele Rodrigo , Gleiser Pablo M. , Forcato Cecilia 
Published: November 2023

AbstractDreaming is a complex phenomenon that occurs during sleep, involving various conscious dream experiences. Lucid dreams (LDs) involve heightened awareness within the dream environment, while out-of-body experiences (OBEs) involve the sensation of being outside one’s physical body. OBEs occur during sleep paralysis (SP), where voluntary movements are inhibited during sleep/wake transitions while remaining aware of the surroundings. The relationship between LDs and OBEs is debated, with some viewing them as distinct phenomena and others considering them different manifestations of the same underlying experience. This study aimed to characterize non-lucid dreams, LDs, and OBEs by analyzing dream reports’ structural properties. OBE reports displayed a condensed and interconnected network structure compared to non-lucid dreams and LDs. Additionally, OBE reports exhibited a specialized network structure, with specific nodes playing a more central role. These findings suggest that OBE dreams may have a more coherent and unified narrative, with certain nodes being pivotal in the network structure.
Comparative performance analysis of binary variants of FOX optimization algorithm with half-quadratic ensemble ranking method for thyroid cancer detection
Authors: Sharma Rohit , Mahanti Gautam Kumar , Panda Ganapati , Rath Adyasha , Dash Sujata , Mallik Saurav , Zhao Zhongming 
Published: November 2023

AbstractThyroid cancer is a life-threatening condition that arises from the cells of the thyroid gland located in the neck’s frontal region just below the adam’s apple. While it is not as prevalent as other types of cancer, it ranks prominently among the commonly observed cancers affecting the endocrine system. Machine learning has emerged as a valuable medical diagnostics tool specifically for detecting thyroid abnormalities. Feature selection is of vital importance in the field of machine learning as it serves to decrease the data dimensionality and concentrate on the most pertinent features. This process improves model performance, reduces training time, and enhances interpretability. This study examined binary variants of FOX-optimization algorithms for feature selection. The study employed eight transfer functions (S and V shape) to convert the FOX-optimization algorithms into their binary versions. The vision transformer-based pre-trained models (DeiT and Swin Transformer) are used for feature extraction. The extracted features are transformed using locally linear embedding, and binary FOX-optimization algorithms are applied for feature selection in conjunction with the Naïve Bayes classifier. The study utilized two datasets (ultrasound and histopathological) related to thyroid cancer images. The benchmarking is performed using the half-quadratic theory-based ensemble ranking technique. Two TOPSIS-based methods (H-TOPSIS and A-TOPSIS) are employed for initial model ranking, followed by an ensemble technique for final ranking. The problem is treated as multi-objective optimization task with accuracy, F2-score, AUC-ROC and feature space size as optimization goals. The binary FOX-optimization algorithm based on the $$V_1$$

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1

transfer function achieved superior performance compared to other variants using both datasets as well as feature extraction techniques. The proposed framework comprised a Swin transformer to extract features, a Fox optimization algorithm with a V1 transfer function for feature selection, and a Naïve Bayes classifier and obtained the best performance for both datasets. The best model achieved an accuracy of 94.75%, an AUC-ROC value of 0.9848, an F2-Score of 0.9365, an inference time of 0.0353 seconds, and selected 5 features for the ultrasound dataset. For the histopathological dataset, the diagnosis model achieved an overall accuracy of 89.71%, an AUC-ROC score of 0.9329, an F2-Score of 0.8760, an inference time of 0.05141 seconds, and selected 12 features. The proposed model achieved results comparable to existing research with small features space.
RETRACTED ARTICLE: Sciadopitysin mitigates spermatological and testicular damage instigated by paraquat administration in male albino rats
Authors: Ijaz Muhammad Umar , Qamer Mohammad , Hamza Ali , Ahmed Hussain , Afsar Tayyaba , Abulmeaty Mahmoud , Ayub Arusha , Razak Suhail 
Published: November 2023

AbstractParaquat (PQ) is a herbicide that has ability to induce testicular toxicity by producing reactive oxygen species (ROS). Sciadopitysin (SPS) is a promising flavonoid that displays multiple pharmacological properties i.e., anti-inflammatory, anti-oxidant and anti-apoptotic. Therefore, the present study was designed to evaluate the mitigative role of SPS against PQ induced testicular toxicity in male rats. The experiment was performed on male albino rats (n = 48) that were divided into 4 groups. The group-1 was control group. Group-2 was administrated orally with PQ (5 mg/kg). Group-3 was administrated orally with PQ (5 mg/kg) and SPS (2 mg/kg). Group-4 was supplemented with SPS (2 mg/kg) through oral gavage. The experiment was conducted for 56 days. The exposure to PQ significantly lowered the activities of catalase (CAT), glutathione reductase (GSR), superoxide dismutase (SOD) as well as glutathione peroxidase (GPx). Whereas, a substantial increase was observed in dead sperms number, abnormalities in the tail, head as well as midpiece of sperms in PQ intoxicated rats. Moreover, a significant increase in the level of ROS and malondialdehyde (MDA) was noticed in PQ administrated group. Furthermore, steroidogenic enzymes expression was significantly decreased in PQ-intoxicated group, whereas the level of inflammatory markers was increased in PQ administrated rats. Besides, the expression of apoptotic markers was significantly escalated in PQ exposed rats, whereas the expression of anti-apoptotic markers was considerably reduced. A significant reduction in hormonal level was also noticed in the rats that were administrated with PQ. Moreover, the histopathological examination revealed that PQ significantly damaged the testicles. However, the supplementation of SPS with PQ significantly reduced the adverse effects of PQ in the testes of albino rats. Therefore, the current investigation demonstrated that SPS possesses a significant potential to avert PQ-induced testicular dysfunction due to its anti-apoptotic, androgenic, anti-oxidant and anti-inflammatory nature.
The association of copeptin with metabolic risk markers is modified by region of origin
Authors: Franzén Anna , Pikkemaat Miriam , Melander Olle , Bennet Louise , Enhörning Sofia 
Published: November 2023

AbstractIraqi born immigrants in Sweden have higher prevalence of metabolic diseases compared to native Swedes. Copeptin, a marker for vasopressin, is associated with increased risk of metabolic disease. In this cross-sectional population study based on the MEDIM cohort we investigated differences in copeptin levels between Iraqi and Swedish born individuals and if the association between copeptin and cardiometabolic risk markers differed by region of origin. We included 1109 Iraqi and 613 Swedish born participants (58% men, mean age 47 years). The Swedish participants had a higher concentration of copeptin compared to the Iraqi born group after age and sex adjustment (p < 0.001). This difference existed only among male individuals with the highest copeptin concentrations, i.e. belonging to copeptin quartile 4 (median (25th; 75th percentile) 20.07 (15.27;33.28) pmol/L for the Swedish born versus 15.57 (13.91;19.00) pmol/L for the Iraqi born, p < 0.001). We found a significant interaction between copeptin (continuous ln-transformed) and being born in Iraq regarding the association with plasma triglycerides (Pinteraction = 0.006). The association between copeptin and BMI was stronger amongst the Iraqi born individuals compared to the Swedish born. Together, this could indicate that copeptin is a more potent marker of metabolic disease among individuals born in Iraq compared to Sweden.
Human leukocyte antigen-DQ risk heterodimeric haplotypes of left ventricular dysfunction in cardiac sarcoidosis: an autoimmune view of its role
Authors: Yamamoto Hironori , Miyashita Yohei , Minamiguchi Hitoshi , Hosomichi Kazuyoshi , Yoshida Shohei , Kioka Hidetaka , Shinomiya Haruki , Nagata Haruno , Onoue Kenji , Kawasaki Masato , Kuramoto Yuki , Nomura Akihiro , Toma Yuichiro , Watanabe Tetsuya , Yamada Takahisa , Ishihara Yasuki , Nagata Miho , Kato Hisakazu , Hakui Hideyuki , Saito Yoshihiko , Asano Yoshihiro , Sakata Yasushi 
Published: November 2023

AbstractCardiac sarcoidosis (CS) is the scarring of heart muscles by autoimmunity, leading to heart abnormalities and patients with sarcoidosis with cardiac involvements have poor prognoses. Due to the small number of patients, it is difficult to stratify all patients of CS by human leukocyte antigen (HLA) analysis. We focused on the structure of antigen-recognizing pockets in heterodimeric HLA-class II, in addition to DNA sequences, and extracted high-affinity combinations of antigenic epitopes from candidate autoantigen proteins and HLA. Four HLA heterodimer-haplotypes (DQA1*05:03/05:05/05:06/05:08-DQB1*03:01) were identified in 10 of 68 cases. Nine of the 10 patients had low left ventricular ejection fraction (< 50%). Fourteen amino-acid sequences constituting four HLA anchor pockets encoded by the HLA haplotypes were all common, suggesting DQA1*05:0X-DQB1*03:01 exhibit one group of heterodimeric haplotypes. The heterodimeric haplotypes recognized eight epitopes from different proteins. Assuming that autoimmune mechanisms might be activated by molecular mimicry, we searched for bacterial species having peptide sequences homologous to the eight epitopes. Within the peptide epitopes form the SLC25A4 and DSG2, high-homology sequences were found in Cutibacterium acnes and Mycobacterium tuberculosis, respectively. In this study, we detected the risk heterodimeric haplotypes of ventricular dysfunction in CS by searching for high-affinity HLA-class II and antigenic epitopes from candidate cardiac proteins.
Novel RICTOR amplification harbouring entities: FISH validation of RICTOR amplification in tumour tissue after next-generation sequencing
Authors: Sztankovics Dániel , Krencz Ildikó , Moldvai Dorottya , Dankó Titanilla , Nagy Ákos , Nagy Noémi , Bedics Gábor , Rókusz András , Papp Gergő , Tőkés Anna-Mária , Pápay Judit , Sápi Zoltán , Dezső Katalin , Bödör Csaba , Sebestyén Anna 
Published: November 2023

AbstractAlterations in mTOR signalling molecules, including RICTOR amplification, have been previously described in many cancers, particularly associated with poor prognosis. In this study, RICTOR copy number variation (CNV) results of diagnostic next-generation sequencing (NGS) were analysed in 420 various human malignant tissues. RICTOR amplification was tested by Droplet Digital PCR (ddPCR) and validated using the “gold standard” fluorescence in situ hybridisation (FISH). Additionally, the consequences of Rictor protein expression were also studied by immunohistochemistry. RICTOR amplification was presumed in 37 cases with CNV ≥ 3 by NGS, among these, 16 cases (16/420; 3.8%) could be validated by FISH, however, ddPCR confirmed only 11 RICTOR-amplified cases with lower sensitivity. Based on these, neither NGS nor ddPCR could replace traditional FISH in proof of RICTOR amplification. However, NGS could be beneficial to highlight potential RICTOR-amplified cases. The obtained results of the 14 different tumour types with FISH-validated RICTOR amplification demonstrate the importance of RICTOR amplification in a broad spectrum of tumours. The newly described RICTOR-amplified entities could initiate further collaborative studies with larger cohorts to analyse the prevalence of RICTOR amplification in rare diseases. Finally, our and further work could help to improve and expand future therapeutic opportunities for mTOR-targeted therapies.
Induced alternative splicing an opportunity to study PCSK9 protein isoforms at physiologically relevant concentrations
Authors: Cale Jessica M. , Ham Kristin A. , Li Dunhui , McIntosh Craig S. , Watts Gerald F. , Wilton Steve D. , Aung-Htut May T. 
Published: November 2023

AbstractSplice modulating antisense oligomers (AOs) are increasingly used to modulate RNA processing. While most are investigated for their use as therapeutics, AOs can also be used for basic research. This study examined their use to investigate internally and terminally truncated proprotein convertase subtilisin/kexin type 9 (PCSK9) protein isoforms. Previous studies have used plasmid or viral-vector-mediated protein overexpression to study different PCSK9 protein isoforms, creating an artificial environment within the cell. Here we designed and tested AOs to remove specific exons that encode for PCSK9 protein domains and produced protein isoforms at more physiologically relevant levels. We evaluated the isoforms’ expression, secretion, and subsequent impact on the low-density lipoprotein (LDL) receptor and its activity in Huh-7 cells. We found that modifying the Cis-His-rich domain by targeting exons 10 or 11 negatively affected LDL receptor activity and hence did not enhance LDL uptake although the levels of LDL receptor were increased. On the other hand, removing the hinge region encoded by exon 8, or a portion of the prodomain encoded by exon 2, have the potential as therapeutics for hypercholesterolemia. Our findings expand the understanding of PCSK9 isoforms and their impact on the LDL receptor and its activity at physiologically relevant concentrations.
Comparative genomics and genome-wide SNPs of endangered Eld’s deer provide breeder selection for inbreeding avoidance
Authors: Pumpitakkul Vichayanee , Chetruengchai Wanna , Srichomthong Chalurmpon , Phokaew Chureerat , Pootakham Wirulda , Sonthirod Chutima , Nawae Wanapinun , Tongsima Sissades , Wangkumhang Pongsakorn , Wilantho Alisa , Utara Yongchai , Thongpakdee Ampika , Sanannu Saowaphang , Maikaew Umaporn , Khuntawee Suphattharaphonnaphan , Changpetch Wirongrong , Phromwat Phairot , Raschasin Kacharin , Sarnkhaeveerakul Phunyaphat , Supapannachart Pannawat , Buthasane Wannapol , Pukazhenthi Budhan S. , Koepfli Klaus-Peter , Suriyaphol Prapat , Tangphatsornruang Sithichoke , Suriyaphol Gunnaporn , Shotelersuk Vorasuk 
Published: November 2023

AbstractEld’s deer, a conserved wildlife species of Thailand, is facing inbreeding depression, particularly in the captive Siamese Eld’s deer (SED) subspecies. In this study, we constructed genomes of a male SED and a male Burmese Eld’s deer (BED), and used genome-wide single nucleotide polymorphisms to evaluate the genetic purity and the inbreeding status of 35 SED and 49 BED with limited pedigree information. The results show that these subspecies diverged approximately 1.26 million years ago. All SED were found to be purebred. A low proportion of admixed SED genetic material was observed in some BED individuals. Six potential breeders from male SED with no genetic relation to any female SED and three purebred male BED with no relation to more than 10 purebred female BED were identified. This study provides valuable insights about Eld’s deer populations and appropriate breeder selection in efforts to repopulate this endangered species while avoiding inbreeding.
Effect of N-phenyl substituent on thermal, optical, electrochemical and luminescence properties of 3-aminophthalimide derivatives
Authors: Kotowicz Sonia , Małecki Jan Grzegorz , Cytarska Joanna , Baranowska-Łączkowska Angelika , Siwy Mariola , Łączkowski Krzysztof Z. , Szalkowski Marcin , Maćkowski Sebastian , Schab-Balcerzak Ewa 
Published: November 2023

AbstractThe seven N-phthalimide derivatives substituted with the amine group at the 3-C position in the phenylene ring were synthesized. The effect of N-substituent chemical structure was investigated. The thermal, electrochemical and optical studies were performed and supported by the density functional theory calculations (DFT). The electrochemical investigations of the synthesized low-molecular phthalimides revealed the one oxidation and reduction process with the HOMO energy level under − 5.81 eV and energy-band gap below 3 eV. The N-phthalimide derivatives were emitted light in a blue spectral region in solutions (in polar and non-polar) with the quantum yield between 2 and 68%, dependent on the substituent at the nitrogen atom, solvent and concentration. The N-phthalimide derivatives were emissive also in a solid state as a thin film and powder. They were tested as a component of the active layer with PVK:PBD matrix and as an independent active layer in the organic light-emitting diodes. The registered electroluminescence spectra exhibited the maximum emission band in the 469–505 nm range, confirming the possibility of using N-phthalimides with PVK:PBD matrix as the blue emitters.
DNA barcode reveals occurrence of threatened species and hidden diversity on Teleost fish trade in the Coastal Amazon
Authors: Santana Paula , Martins Thais , Lutz Ítalo , Miranda Josy , da Silva Raimundo , Mesquita David , Martins Rita , Veneza Ivana , Vallinoto Marcelo , Sampaio Iracilda , Evangelista-Gomes Grazielle 
Published: November 2023

AbstractThis study aimed to identify the teleost fish species sold in Bragança, a major fishing hub on the north coast of Brazil. The COI gene analysis was performed for the identification of fish species. The local market uses common names that are not accurate and do not reflect the diversity of the species. 204 sequences were obtained, with 119 haplotypes. 83 species were identified by comparing with public databases and constructing phylogenetic trees, with Carangidae being the most prevalent family. The study also foundHaemulon atlanticus,Menticirrhus cuiaranensisandHoplias misioneira, a newly described species from the Amazon basin, among the samples. Additionally, 73 commercial names were recorded, including 10 categories, and the illegal trade ofEpinephelus itajarawas detected. The DNA Barcode method proved to be effective for discriminating the species. The study highlights that common and commercial names are vague and underestimate the fish diversity, and that Brazil needs to revise its regulations for commercial and scientific names.
The role of MOTS-c-mediated antioxidant defense in aerobic exercise alleviating diabetic myocardial injury
Authors: Tang Mi , Su Quansheng , Duan Yimei , Fu Yu , Liang Min , Pan Yanrong , Yuan Jinghan , Wang Manda , Pang Xiaoli , Ma Jiacheng , Laher Ismail , Li Shunchang 
Published: November 2023

AbstractMyocardial remodeling and dysfunction are commonly observed in type 2 diabetes mellitus (T2DM). Aerobic exercise can partly alleviate diabetes-induced myocardial dysfunction through its antioxidant actions. MOTS-c is a potential exercise mimic. This study aimed to investigate the effects of MOTS-c on improving diabetic heart function and its mechanism and to identify whether MOTS-c improved antioxidant defenses due to aerobic exercise. Herein, we established a rat model of T2DM induced by high-fat diet combined with a low-dose streptozotocin injection. Interventions were performed using intraperitoneal injections of MOTS-c (i.p. 0.5 mg/kg/day, 7 days/week) or aerobic exercise training (treadmill, 20 m/min, 60 min/day, 5 days/week) for 8 weeks. Myocardial ultrastructure was assessed using transmission electron microscopy (TEM), myocardial lipid peroxidation levels (MDA), superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) levels were assessed using colorimetric methods, and molecular analyses including MOTS-c, Kelch-like ECH-associated protein 1 (Keap1), Nuclear factor E2-related factor 2 (Nrf2), adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)and phospho-AMPK (p-AMPK) were examined using Western blot. The results showed that MOTS-c, with or without exercise, reduced myocardial ultrastructural damage and improved glucolipid metabolism and cardiac function in T2DM. Furthermore, MOTS-c increased antioxidant markers such as SOD, CAT, and the protein expression of myocardial MOTS-c, Keap1, Nrf2, and p-AMPK. MOTS-c with exercise treatment reduced myocardial MDA and increased p-AMPK significantly comparing to only exercise or MOTS-c alone. Our findings suggest that MOTS-c may be a helpful supplement for overcoming exercise insufficiency and improving myocardial structure and function in diabetes.
Effects and mechanisms of intrauterine chronic hypoxia on ovarian reserve function of zygotic rats
Authors: Huang Yanyan , Su Shanshan , Luo Weiwen , Zhong Huohu , Wang Xiali , Lyu Guorong 
Published: November 2023

AbstractChronic intrauterine hypoxia (ICH) may lead to permanent alterations in the offspring's body structure, function, and metabolism through the "developmental programming" pathway, resulting in lasting changes in physiology and metabolism, as well as the onset of adult-onset diseases. The aim was to investigate intrauterine growth restriction caused by ICH and its effect on ovarian reserve function in female offspring at different developmental stages after birth. Healthy female Sprague–Dawley rats (n = 20) were pregnant by normal mating, and the rats in the ICH group were treated with chronic intrauterine hypoxia twice a day for 04 h00 each time from day 4 to 21 of gestation. After the first hypoxic treatment, four pregnant rats were randomly selected from the ICH and natural control groups for arterial blood gas analysis. In the ICH group, birth weight and body weight on the 5th day after birth were less than in the control group, the total number of follicles and the number of primordial follicles in the offspring of the ICH group were significantly reduced on postnatal days 5, 20, and 40 (p < 0.05). ICH decreases ovarian reserve function in female offspring rats and programmatically regulates the differential expression of ovarian miRNAs in female offspring rats.