bluuebunny/crossref_metadata_2025_split huggingface dataset:
Triptolide Reduces MDA‐MB‐231 Cell Metastasis by Attenuating Epithelial‐Mesenchymal Transition through the ROCK/PTEN/Akt Axis
Authors: Wu Qinhang , Leng Xuejiao , Ma Xuelin , Xu Qixuan , Ni Ruijun , Pan Yang , Jin Zhuolin , Kalim Muhammad 
Published: November 2023

AbstractTriple‐negative breast cancer (TNBC) is a highly heterogeneous and invasive subtype of breast cancer. The prognosis of TNBC is poor because of its high distant metastasis rate. Triptolide is a type of diterpene trioxide natural compound with potential anti‐tumor activities. This study explored the metastatic inhibitory effect of triptolide on MDA‐MB‐231 cells and its underlying mechanism. Triptolide suppressed cell proliferation and induced cell apoptosis in a time‐ and dose‐dependent manner. Low doses of triptolide (0‐8 nM) reduced the migration and invasion capabilities of MDA‐MB‐231 cells. Triptolide decreased ROCK1, p‐Akt, N‐cadherin, vimentin and MMP‐9 expressions, but increased PTEN and E‐cadherin expressions on protein and mRNA levels. Furthermore, the down‐regulation of ROCK1 expression in MDA‐MB‐231 cells after being treated by triptolide could be rescued by ROCK1 specific inhibitor Y27632. Molecular docking showed that triptolide and Y27632 shared the same active center of ROCK1 protein. This article‘s findings taken together showed that ROCK1 is the primary target of triptolide, which can cause cell apoptosis and inhibit the epithelial‐mesenchymal transition of MDA‐MB‐231 cells.
Ferruginoside D: A Novel Phenylethanoid from Verbascum leiocarpum
Authors: Şimşek Samed , Akşit Hüseyin , Aydın Ali , Köksal Ekrem 
Published: November 2023

AbstractA novel phenylethanoid, ferruginoside D, together with fourteen known compounds were isolated from the Verbascum leiocarpum for the first time. Chemical structures of isolated compounds were determined by spectroscopic analysis including HR‐ESI‐MS and NMR spectra. The antioxidant, α‐glucosidase inhibitory properties, and antiproliferative activities against multiple cell lines (A172, C6, HeLa, A2780, SW620, HT29, Beas2B, RPE, and HSF) of the isolated compounds were evaluated in vitro. According to the results, iridoids, flavonoids (luteolin and apigenin), and phenylethanoids (poliumoside and isoverbascoside) with strong antiproliferative potential were also found to have cytostatic effects. Furthermore, the investigation revealed that compounds luteolin, poliumoside, verbascoside, isoverbascoside, ferruginoside C, ferruginoside D, and ursolic acid show potent alpha‐glucosidase inhibitory activity, while compounds luteolin, verbascoside, and isoverbascoside exhibit substantial antioxidant activity. The new compound (ferruginoside D) was found moderately active against cancer cell lines, with strong alpha‐glucosidase inhibitory activity, and moderate antioxidant properties.
Egg white improved the quality of noodles with high mung bean content by protein aggregation behavior
Authors: Li Ruoning , Wang Li , Hou Dianzhi , Zhou Sumei 
Published: November 2023

AbstractBackground and ObjectivesMung bean, as a coarse grain, is rich in protein and phytochemicals. Noodles prepared from mung bean are nutritionally superior to traditional noodles. However, the absence of gluten protein in mung bean limits their incorporation level and might cause adverse effects on the quality of noodles. To address the quality degradation of noodles with high mung bean content due to the lack of gluten proteins, the effects of fresh egg white on their quality improvement were investigated. In this study, the dynamic rheological properties of the dough as well as the changes in free sulfhydryl and SS bonds were determined. The cooking characteristics, sensory, textural properties, and microstructure of the noodles were evaluated.FindingsResults revealed that the addition of 15% fresh egg white significantly improved the sensory scores of the wheat–mung bean (3:7) composite noodles, which might be attributed to the enhanced modulus of elasticity (G′) of the composite dough system, the increased hydrophilicity, and amount of free sulfhydryl and SS bonds in noodles. Meanwhile, scanning electron microscopy and confocal laser scanning microscopy further confirmed that the addition of 15% fresh egg white affected the structures of starch granule and protein network. Furthermore, the addition of 15% fresh egg white minimized the cooking loss and enhanced the springiness, adhesiveness, and chewiness of the noodles, which in turn resulted in the highest sensory scores.ConclusionsThe incorporation of 15% fresh egg white could enhance the quality characteristics of mung bean noodles by protein aggregation, thus receiving a favorable acceptance.Significance and NoveltyThis study explored the possibility of developing a novel noodle with 70% mung bean content. Moreover, the addition of 15% fresh egg white could significantly improve its quality and overall acceptability. This result contributes to the development of high‐content gluten‐free cereal or other legume noodles.
Kdm7a expression is spatiotemporally regulated in developing Xenopus laevis embryos, and its overexpression influences late retinal development
Authors: Martini Davide , Digregorio Matteo , Voto Ilaria Anna Pia , Morabito Giuseppe , Degl'Innocenti Andrea , Giudetti Guido , Giannaccini Martina , Andreazzoli Massimiliano 
Published: November 2023

AbstractBackgroundPost‐translational histone modifications are among the most common epigenetic modifications that orchestrate gene expression, playing a pivotal role during embryonic development and in various pathological conditions. Among histone lysine demethylases, KDM7A, also known as KIAA1718 or JHDM1D, catalyzes the demethylation of H3K9me1/2 and H3K27me1/2, leading to transcriptional regulation. Previous data suggest that KDM7A plays a central role in several biological processes, including cell proliferation, commitment, differentiation, apoptosis, and maintenance. However, information on the expression pattern of KDM7A in whole organisms is limited, and its functional role is still unclear.ResultsIn Xenopus development, kdm7a is expressed early, undergoing spatiotemporal regulation in various organs and tissues, including the central nervous system and the eye. Focusing on retinal development, we found that kdm7a overexpression does not affect the expression of genes critically involved in early neural development and eye‐field specification, whereas unbalances the distribution of neural cell subtypes in the mature retina by disfavoring the development of ganglion cells while promoting that of horizontal cells.ConclusionsKdm7a is dynamically expressed during embryonic development, and its overexpression influences late retinal development, suggesting a potential involvement in the molecular machinery regulating the spatiotemporally ordered generation of retinal neuronal subtypes.
Calm after the storm? Similar patterns of genetic variation in a riverine foundation species before and after severe disturbance
Authors: Ngeve Magdalene N. , Engelhardt Katharina A. M. , Gray Michelle , Neel Maile C. 
Published: November 2023

AbstractIn summer 2011, Tropical storms Lee and Irene caused an estimated 90% decline of the submersed aquatic plant Vallisneria americana Michx. (Hydrocharitaceae) in the Hudson River of New York (USA). To understand the genetic impact of such large‐scale demographic losses, we compared diversity at 10 microsatellite loci in 135 samples collected from five sites just before the storms with 239 shoots collected from nine sites 4 years after. Although 80% of beds sampled in 2011 lacked V. americana in 2015, we found similar genotypic and genetic diversity and effective population sizes in pre‐storm versus post‐storm sites. These similarities suggest that despite local extirpations concentrated at the upstream end of the sampling area, V. americana was regionally resistant to genetic losses. Similar geographically based structure among sites in both sampling periods suggested that cryptic local refugia at previously occupied sites facilitated re‐expansion after the storms. However, this apparent resistance to disturbance may lead to a false sense of security. Low effective population sizes and high clonality in both time periods suggest that V. americana beds were already small and had high frequency of asexual reproduction before the storms. Dispersal was not sufficient to recolonize more isolated sites that had been extirpated. Chronic low diversity and reliance on asexual reproduction for persistence can be risky when more frequent and intense storms are paired with ongoing anthropogenic stressors. Monitoring genetic diversity along with extent and abundance of V. americana will give a more complete picture of long‐term potential for resilience.
α‐CF3‐Substituted Saturated Bicyclic Amines: Advanced Building Blocks for Medicinal Chemistry
Authors: Smyrnov Oleh , Melnykov Kostiantyn P. , Semeno Volodymyr , Liashuk Oleksandr S. , Grygorenko Oleksandr O. 
Published: November 2023

AbstractA straightforward approach to α‐CF3‐substituted saturated bicyclic amines starting from commercially available compounds is proposed. The key steps include addition of the Ruppert‐Prakash reagent to imine moiety and AlMe3‐promoted intramolecular heterocyclization. The reaction sequence provides access to fluorine‐containing azabicyclo[n.2.1]alkane (n=1–3) or azabicyclo[2.2.2]octane derivatives in 10–42 % overall yield. The physicochemical properties of the prepared compounds or their model derivatives (i. e., pKa and LogP) were measured and compared to those for the parent monocyclic or non‐fluorinated saturated heterocycles. As expected, amine basicity was lowered considerably upon introduction of the CF3 group (ΔpKa≈4), while the lipophilicity increased (by ca. 0.5 LogP units). The summarized data provides a convenient guideline for application of the synthesized compounds in drug discovery programs.
Organocatalyzed Kinetic Resolution of Racemic Carboxylic Acids
Authors: Okuno Ken , Furuya Yasuaki , Shirakawa Seiji 
Published: November 2023

AbstractThe kinetic resolution of racemic compounds is a reliable method to prepare important chiral molecules in highly optically enriched forms. Although methods for the catalytic kinetic resolution of chiral alcohols, amines, and epoxides have been extensively investigated, the kinetic resolution of racemic carboxylic acids has remained under‐developed despite the importance of chiral carboxylic acids. Excellent catalytic approaches for the kinetic resolution of racemic carboxylic acids via esterification using chiral organocatalysts have been reported by several research groups. Furthermore, the organocatalyzed lactonization of alkenyl and alkynyl carboxylic acids has also been adapted to achieve kinetic resolution. Herein, the methods typically used to produce the organocatalyzed kinetic resolution of racemic carboxylic acids are reviewed to elucidate the current status of this research.
Evolutionary medicine approaches to chronic disease: The case of irritable bowel syndrome
Authors: Lenover Makenna B. , Shenk Mary K. 
Published: November 2023

AbstractIrritable bowel syndrome (IBS), a gastrointestinal disease, is a global phenomenon correlated with industrialization. We propose that an evolutionary medicine approach is useful to understand this disease from an ultimate perspective and conducted a scoping literature review to synthesize the IBS literature within this framework. Our review suggests five potential evolutionary hypotheses for the cause of IBS, including (a) a dietary mismatch accompanying a nutritional transition, (b) an early hygienic life environment leading to the immune system and microbiotic changes, (c) an outcome of decreased physical activity, (d) a response to changes in environmental light–dark cycles, and (e) an artifact of an evolved fight or flight response. We find key limitations in the available data needed to understand early life, nutritional, and socioeconomic experiences that would allow us to understand evolutionarily relevant risk factors and identify a need for further empirical research to distinguish potential causes and test evolutionary hypotheses.
Prognostic role of the MRI‐based involvement of superior pharyngeal constrictor muscle in oropharyngeal squamous cell carcinoma
Authors: Tirelli Giancarlo , Marcuzzo Alberto Vito , Gardenal Nicoletta , Tofanelli Margherita , Degrassi Ferruccio , Cova Maria Assunta , Sacchet Erika , Giudici Fabiola , Polesel Jerry , Boscolo‐Rizzo Paolo 
Published: November 2023

AbstractObjectivesThe aim of this study was to determine the impact of the involvement of the superior pharyngeal constrictor muscle (SPCM) evaluated by magnetic resonance imaging (MRI) on outcome in oropharyngeal squamous cell carcinomas (OPSCCs).MethodsA retrospective study including consecutive patients with OPSCC treated with curative intent.ResultsA total of 82 consecutive patients with OPSCC met inclusion criteria. At multivariate analysis, patients with SPCM infiltration were at significantly higher risk of death (HR: 3.37, CI: 1.21–9.38) and progression (HR: 3.39, CI: 1.38–8.32). In a multivariate model conditioned on HPV status, a significantly higher risk of death and progression was observed by combining both SPCM and HPV status with patients harboring an HPV‐negative OPSCC with SPCM infiltration showing the poorest outcome.ConclusionMRI evidence of SPCM involvement significantly and independently increases the risk of death and progression in subjects with OPSCC. Considering both MRI‐assessed SPCM infiltration and HPV status significantly improved risk stratification in these malignancies.
Designing a minimum data set for the information management system (registry) of spinal canal stenosis: An applied‐descriptive study
Authors: Zarei Javad , Mohammadi Ali , Akrami Mohamad Reza , Jeihooni Kalhori Azar 
Published: November 2023

AbstractBackground and AimsSpinal canal stenosis is one of the most common vertebral column diseases, which can lead to disability. Developing a registry system can help in research on the prevention and effective treatment of it. This study designs a minimum data set (MDS) as the first step in creating a registry system for spinal canal stenosis.MethodThe present research is of applied‐descriptive type, performed in 2022. First, the applicable data elements about the disease were selected from a vast range of English and Farsi references, including peer reviewed articles, academic books, credible websites, and medical records of hospitalized patients. Through the extracted data, the primary MDS plan was designed as a questionnaire. The validity of the questionnaire was conducted via asking the opinion of experts (neurosurgeons, physiotherapists, epidemiologists, and health information management specialists). Also, its reliability was calculated via Cronbach ⍺ coefficient, which was 86%. Finally, the MDS of the spinal canal stenosis national registry system (for Iran) was confirmed through a two stage Delphi technique. Data analysis was applied through descriptive statistics via SPSS21 software.ResultsThe proposed MDS is offered in two general sets of data: administrative and clinical. For the administrative data set, 40 data elements had been proposed, as five classes. Twenty‐six of them were confirmed. In the clinical section, 95 data elements had been proposed in 14 classes; 94 of which were finally confirmed.ConclusionSince there is no spinal canal stenosis MDS available, this study can be a turning point in the standardization of the data on this disease. Moreover, these precise, coherent, and standard data elements can be contributed to improving disease management and enhancing the public healthcare quality. Also, the MDS proposed in this study can help researchers and experts, design a spinal canal stenosis registry system in other countries.
Removal of ochratoxin A in wine by Cryptococcus albidus and safety assessment of degradation products
Authors: Wang Leran , Cai Rui , Zhang Jierong , Liu Xiaoshuang , Wang Saiqun , Ge Qian , Zhao Zidan , Yue Tianli , Yuan Yahong , Wang Zhouli 
Published: November 2023

AbstractBACKGROUNDOchratoxin A (OTA) is a mycotoxin that contaminates grape‐based products and is extremely harmful to the health of the host. It is effectively removed by yeast during the fermentation of wine, whereas the removal mechanism of OTA remains unclear. Therefore, the present study aimed to investigate the removal mechanism of ochratoxin A by yeast and to evaluate the safety of its degradation products.RESULTSCryptococcus albidus (20‐G) with better effect on ochratoxin A (OTA) was screened out in the main fermentation stage of wine. The results showed that 20‐G removed OTA through biosorption and biodegradation. Intracellular enzymes played the main role (18.44%) and yeast cell walls adsorbed a small amount of OTA (8.44%). Furthermore, the identification of proteins in 20‐G revealed that the decrease in OTA content was mainly a result of the action of peroxidase, and validation tests were carried out. By analyzing the degradation products of OTA, OTα and phenylalanine with lower toxicity were obtained. Animal experiments showed that the intervention of yeast 20‐G reduced the damage and adverse effects caused by OTA toxicity to the mice.CONCLUSIONThe present study demonstrates the mechanism of OTA removal by 20‐G and the toxicity of OTA was reduced by peroxidase in 20‐G. © 2023 Society of Chemical Industry.
User preferences and consumer acceptability of boiled plantain in rural and urban localities in Cameroon
Authors: Ngoh Newilah Gérard , Kendine Vepowo Cédric , Nya Nzimi Raymonde , Kuate Kengne Cédric , Takam Ngouno Annie , Nana Claudin Karim , Meli Meli Vivien , Gouado Inocent , Dufour Dominique , Mbeguie‐A‐Mbeguie Didier 
Published: November 2023

AbstractBACKGROUNDAn understanding of the preferences of different stakeholders in the plantain value chain in rural and urban segments in Cameroon is important for the selection and adoption of new plantain cultivars. Boiled plantain is one of the most commonly consumed food products from this crop in Cameroon. Gendered food mapping and consumer testing with two plantain landraces (Batard and Big Ebanga) and a plantain‐like hybrid (CARBAP K74) was carried out in rural and urban areas in the West and Littoral regions of Cameroon.RESULTSPlantain users in these two regions were categorized into producers, traders, processors, and consumers. Preferences indicated that raw plantain should bear long and large fruits, with heavy bunches, and an orange pulp color, whereas boiled plantain should present with a yellow color and a soft and mealy pulp, with a good plantain aroma. Batard and Big Ebanga were liked moderately by consumers, whereas CARBAP K74 was liked slightly. CARBAP K74 was on par with Batard and Big Ebanga for some attributes, except for color and sweetness.CONCLUSIONMore emphasis should be given to attributes such as color, firmness, and taste for the development of new plantain clones to be adopted by end users consuming boiled green plantain. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
The limits of awards for anti‐corruption: Experimental and ethnographic evidence from Uganda
Authors: Buntaine Mark T. , Bagabo Alex , Bangerter Tanner , Bukuluki Paul , Daniels Brigham 
Published: November 2023

AbstractConventional anti‐corruption approaches focus on detecting and punishing the misuse of public office. These approaches are often ineffective in settings where social norms do not support reporting and punishing corruption. Attempting to build anti‐corruption norms, we conducted a field experiment in Uganda that offered elected, local leaders the chance to earn awards for overseeing development projects according to legal guidelines. We then conducted a second field experiment that informed other leaders and the public about the award winners. Offering leaders the chance to earn recognition did not improve the management of projects or change leaders’ norms about corruption. Informing other leaders and residents about the award winners also did not change behaviors or attitudes related to corruption. A paired ethnographic study shows that the possibility for recognition generated excitement but was not able to overcome resignation by local leaders. Our study provides some of the first experimental evidence about using awards to motivate public officials to act with integrity and to build anti‐corruption norms among both leaders and the public. The results imply that awards have limited effects for anti‐corruption in settings with endemic corruption and where they cannot be used instrumentally by awardees.
Additive manufacturing of high‐strength polyamide 6 composites reinforced with continuous carbon fiber prepreg
Authors: Chen Wei , Hu Zhonglue , Li Xiping , He Jiawen , Wang Sisi , Zhao Yuan , Li Mengjia , Zhang Jiazhen 
Published: November 2023

AbstractThe additive manufacturing of continuous fiber reinforced thermoplastics (CFRTPs) paves way for the high‐strength, light‐weight components for variety of load‐bearing applications. In this work, the continuous carbon fiber reinforced PA6 (CCF‐PA6) composites was successfully printed from the prepreg filament. The prepreg filament was prepared in‐house by impregnating the heat‐and‐acid treated 1 K carbon fiber bundle with the molten PA6. The tensile strength of the prepreg filament, which contained with 40 vol% CF, reached 984 MPa. The unidirectional CCF‐PA6 specimens were subsequently 3D‐printed with the prepreg filament, and the mechanical strength of those 3D‐printed specimens were tunable by adjusting a set of printing parameters, such as layer thickness, hatch spacing and printing temperatures. The highest tensile strength of the specimen reached 555 MPa. Those specimens also exhibited outstanding mechanical strength at elevated temperatures, still reaching 184 MPa at 150°C. The mechanical strength of those specimens was dependent on the content of the fiber. This study can hopefully provide new insights for feedstock design and spur novel ideas in tailoring the mechanical properties of the 3D‐printed CFRTPs.
Tertiary amine oxides as perovskite nanocrystal ligands and components of polymeric nanocomposites
Authors: Leone Grace , Cueto Christopher , Hu Mingqiu , Russell Thomas P. , Emrick Todd 
Published: November 2023

AbstractAs a class of semiconductor nanocrystals that exhibit high photoluminescence quantum yield (PLQY) at tunable wavelengths, perovskite nanocrystals (PNCs) are attractive candidates for optoelectronic and light‐emitting devices. However, attempts to optimize PNC integration into such applications suffer from PNC instability and loss of PL over time. Here, we describe the impact of organic and polymeric N‐oxides when used in conjunction with PNCs, whereby a significant increase in PNC quantum yield is observed in solution, and stable PL emission is obtained in polymeric nanocomposites. Specifically, when using aliphatic N‐oxides in ligand exchange with CsPbBr3 PNCs in solution, a substantial boost in PNC brightness is observed (~40% or more PLQY increase), followed by an alteration of the perovskite chemistry. When N‐oxide substituents are positioned pendent to a poly(n‐butyl methacrylate) backbone, the optically clear flexible nanocomposite films obtained have bright PL emission and maintain optical clarity for months. X‐ray diffraction is useful for characterizing the PNC crystalline structure following exposure to aliphatic N‐oxides, while electron microscopy (EM) and small‐angle X‐ray scattering (SAXS) measurements of the PNC‐polymer nanocomposites show this polymeric N‐oxide platform to cleanly disperse PNCs in flexible polymer films.