bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constructing public–private partnerships to undermine the public interest: critical discourse analysis of Working Together published by the International Alliance for Responsible Drinking
Authors: Madden Mary , Bartlett Andrew , McCambridge Jim 
Published: December 2023

AbstractBackgroundThe global burden of alcohol harm has increased and is forecast to grow further without effective policy implementation. Public–private partnerships aiming to address global health, and other societal challenges, are a burgeoning feature of neoliberal governance. Rhetorically distancing themselves from tobacco, the major alcohol companies are committed to tackling ‘harmful drinking’ and have created a distinct type of public relations organization for this purpose. The activities of such organizations are increasingly recognized as an impediment to the implementation of policies to reduce alcohol harm, including in low- and middle-income countries where markets are expanding.MethodsThe approach of critical discourse analysis is used to examine the discursive tactics and strategies used inWorking Together; a ‘toolkit’ published by the key global level alcohol industry public relations organization, the International Alliance for Responsible Drinking (IARD). This study considers how it works discursively to set the terms of, and overcome skepticism about partnerships, to define aims and position various actors by constructing their roles. The construction of prospective partners provides insights into the alcohol industry itself.ResultsThe toolkit operates as an ideological resource for forming public–private partnerships across the world based on the accumulated know-how of the major companies through IARD. This allows the largest alcohol companies to exercise leadership of the industry, while remaining off-stage. The toolkit relies on a form of rhetorical work which creates distance from obvious corporate interests and the harms caused to population health and society. This is accomplished by workingagainstevidence-informed population level approaches, and thus avoiding policies that will make any significant difference to overall alcohol harm. Unspecific “complexity” affords opportunity for preferred types of “actions”, and “partnership” provides opportunity to gain credibility by association, further minimizing the likelihood of any material harm being reduced.ConclusionsThe toolkit is designed to not only legitimate the inclusion of alcohol industry actors as initiating ‘partners’, but also assigns them roles as managers of a set of carefully constructed relationships. This vision of public–private partnership reproduces the hegemonic narrative that has successfully blocked policy advances for decades and led to growing alcohol harm globally.
No evidence of genetic causality between diabetes and osteonecrosis: a bidirectional two-sample Mendelian randomization analysis
Authors: Li Wei , Chai Jin-Lian , Li Zhe , Guo Cong-Cong , Wei Ran , Sun Tie-Feng , Liang Xue-Zhen 
Published: December 2023

Abstract
Objective
This study aimed to examine whether diabetes mellitus is causally associated with osteonecrosis.

Method
Using publicly accessible genome-wide association study statistics, a bidirectional two-sample Mendelian randomization analysis was carried out. In order to determine whether diabetes has a causal effect on osteonecrosis and whether osteonecrosis has a causal effect on diabetes, we extracted six date on diabetes in Europeans from IEU OpenGWAS and GWAS Catalogue and osteonecrosis in Europeans from FinnGen. We then evaluated the data using inverse variance weighting, MR-Egger regression, weighted median, weighted mode, and simple mode. The results’ stability and dependability were then evaluated using sensitivity analysis and heterogeneity analysis. Finally, meta-analysis is used to further confirm if there is a relationship between diabetes and osteonecrosis.

Results
When diabetes was used as an exposure factor, MR-Egger regression showed that directional fold product was unlikely to bias the results. Cochran’s Q test showed only minor heterogeneity in a few data sets. Multidirectional tests Egger-intercept, MR-PRESSO and funnel plots for most data did not show multidirectional and asymmetry at the gene level. Most of the IVW results showed no causal relationship between diabetes mellitus and osteonecrosis. The results of meta-analysis of IVW methods further confirmed the absence of a causal relationship. Inverse MR analysis also showed no causal relationship between osteonecrosis and diabetes.

Conclusion
Results of bidirectional MR analysis show no evidence of causal relationship between diabetes and osteonecrosis.
Harnessing immunotherapy for brain metastases: insights into tumor–brain microenvironment interactions and emerging treatment modalities
Authors: Zhou Dairan , Gong Zhenyu , Wu Dejun , Ma Chao , Hou Lijun , Niu Xiaomin , Xu Tao 
Published: December 2023

AbstractBrain metastases signify a deleterious milestone in the progression of several advanced cancers, predominantly originating from lung, breast and melanoma malignancies, with a median survival timeframe nearing six months. Existing therapeutic regimens yield suboptimal outcomes; however, burgeoning insights into the tumor microenvironment, particularly the immunosuppressive milieu engendered by tumor–brain interplay, posit immunotherapy as a promising avenue for ameliorating brain metastases. In this review, we meticulously delineate the research advancements concerning the microenvironment of brain metastases, striving to elucidate the panorama of their onset and evolution. We encapsulate three emergent immunotherapeutic strategies, namely immune checkpoint inhibition, chimeric antigen receptor (CAR) T cell transplantation and glial cell-targeted immunoenhancement. We underscore the imperative of aligning immunotherapy development with in-depth understanding of the tumor microenvironment and engendering innovative delivery platforms. Moreover, the integration with established or avant-garde physical methodologies and localized applications warrants consideration in the prevailing therapeutic schema.
Characterization of an efficient N-oxygenase from Saccharothrix sp. and its application in the synthesis of azomycin
Authors: Fan Chuanle , Zhou Fang , Huang Wei , Xue Yi , Xu Chao , Zhang Rubing , Xian Mo , Feng Xinjun 
Published: December 2023

Abstract
Background
The nitro group constitutes a significant functional moiety within numerous valuable substances, such as nitroimidazoles, a class of antimicrobial drugs exhibiting broad spectrum activity. Conventional chemical methods for synthesizing nitro compounds suffer from harsh conditions, multiple steps, and environmental issues. Biocatalysis has emerged as a promising alternative to overcome these drawbacks, with certain enzymes capable of catalyzing nitro group formation gradually being discovered in nature. Nevertheless, the practical application is hindered by the restricted diversity and low catalytic activity exhibited by the reported nitrifying enzymes.

Results
A novel N-oxygenase SaRohS harboring higher catalytic capability of transformation 2-aminoimidazole to azomycin was characterized from Saccharothrix sp. Phylogenetic tree analysis revealed that SaRohS belongs to the heme-oxygenase-like diiron oxygenase (HDOs) family. SaRohS exhibited optimal activity at pH 5.5 and 25 ℃, respectively. The enzyme maintained relatively stable activity within the pH range of 4.5 to 6.5 and the temperature range of 20 ℃ to 35 ℃. Following sequence alignment and structural analysis, several promising amino acid residues were meticulously chosen for catalytic performance evaluation. Site-directed mutations showed that threonine 75 was essential for the catalytic activity. The dual mutant enzyme G95A/K115T exhibited the highest catalytic efficiency, which was approximately 5.8-fold higher than that of the wild-type and 22.3-fold higher than that of the reported N-oxygenase KaRohS from Kitasatospora azatica. The underlying catalytic mechanism was investigated through molecular docking and molecular dynamics. Finally, whole-cell biocatalysis was performed and 2-aminoimidazole could be effectively converted into azomycin with a reaction conversion rate of 42% within 14 h.

Conclusions
An efficient N-oxygenase that catalyzes 2-aminoimidazole to azomycin was screened form Saccharothrix sp., its phylogenetics and enzymatic properties were analyzed. Through site-directed mutation, enhancements in catalytic competence were achieved, and the molecular basis underlying the enhanced enzymatic activity of the mutants was revealed via molecular docking and dynamic simulation. Furthermore, the application potential of this enzyme was assessed through whole cell biocatalysis, demonstrating it as a promising alternative method for azomycin production.

Graphical Abstract
Reproducibility of peak moment for isometric and isokinetic knee extension exercise
Authors: Zöger Manfred , Nimmerichter Alfred , Baca Arnold , Wirth Klaus 
Published: December 2023

Abstract
Background
Accurate measurements of muscular performance are important for diagnostics, for example during rehabilitation after traumatic injuries but also in competitive sports. For these purposes, dynamometric devices are widely used and considered the gold standard for muscle strength testing. However, few previous studies have tested the reproducibility of peak moment (PM) at velocities close to the maximum device capability, and in general, reproducibility results cannot be transferred to other devices or test protocols. The purpose of this study was to evaluate the reproducibility of PM for different isometric and isokinetic knee extension exercises using the IsoMed 2000.

Methods
Thirty subjects volunteered in three repeated test sessions, including isometric knee extension (100° and 140° knee angle) and isokinetic knee extension (30°/s and 400°/s). Statistical analysis for comparison of sessions two and three included paired sample t-test, calculation of intraclass correlation coefficient (ICC) and standard error of measurement (SEM). Additionally, Bland Altman statistics and corresponding plots were created.

Results
A significant difference between sessions in PM was found for isometric knee extension in one leg (140° left). Reproducibility was high for all conditions with ICC ranging from 0.964 to 0.988 and SEM in the range of 7.6 to 10.5 Nm. Bland Altman statistics revealed a bias between − 7.3 and 0.7 Nm.

Conclusions
Reproducibility of PM using the IsoMed 2000 was good after an initial familiarization trial with high values of relative reproducibility. Absolute reproducibility can be interpreted as appropriate for most common practical applications.
Cotyledonoid dissecting leiomyoma of the uterus: a case report and review of the literature
Authors: Chahkandi Mahboobeh , Ataei Marzieh , Bina Amir Reza , Mozayani Farnaz , Fanoodi Ali 
Published: December 2023

Abstract
Background
Cotyledonoid dissecting leiomyoma, also named Sternberg tumor, is a rare variant of uterine leiomyoma. The tumor is benign, but the appearance and growth pattern are unusual and alarming. In this article, we report a case of cotyledonoid dissecting leiomyoma in a 55-year-old woman as well as review relevant literature.

Case presentation
We report a case of cotyledonoid dissecting leiomyoma in a 55-year-old Iranian woman who presented with vaginal bleeding 4 months after menopause. Ultrasound showed two heterogeneous hypoechoic masses on the uterine fundus. Total abdominal hysterectomy and bilateral salpingo-oophorectomy were performed for the patient. Macroscopically, a large heterogeneous intramural mass (140 mm × 120 mm × 120 mm) with a grape-like exophytic mass on the fundus was observed. Her health status was good after surgery, and the patient was discharged from the hospital after 2 days. In a 1-year follow-up period, no recurrence or any other related complications were found.

Conclusion
It is important to recognize this rare variant of leiomyoma to prevent aggressive and inappropriate overdiagnosis and overtreatment. It is suggested to try to use frozen sections for better diagnosis and to preserve fertility in young women suffering from this lesion.
Hypoxia enhances human myoblast differentiation: involvement of HIF1α and impact of DUX4, the FSHD causal gene
Authors: Nguyen Thuy-Hang , Paprzycki Lise , Legrand Alexandre , Declèves Anne-Emilie , Heher Philipp , Limpens Maelle , Belayew Alexandra , Banerji Christopher R. S. , Zammit Peter S. , Tassin Alexandra 
Published: December 2023

Abstract
Background
Hypoxia is known to modify skeletal muscle biological functions and muscle regeneration. However, the mechanisms underlying the effects of hypoxia on human myoblast differentiation remain unclear. The hypoxic response pathway is of particular interest in patients with hereditary muscular dystrophies since many present respiratory impairment and muscle regeneration defects. For example, an altered hypoxia response characterizes the muscles of patients with facioscapulohumeral dystrophy (FSHD).

Methods
We examined the impact of hypoxia on the differentiation of human immortalized myoblasts (LHCN-M2) cultured in normoxia (PO2: 21%) or hypoxia (PO2: 1%). Cells were grown in proliferation (myoblasts) or differentiation medium for 2 (myocytes) or 4 days (myotubes). We evaluated proliferation rate by EdU incorporation, used myogenin-positive nuclei as a differentiation marker for myocytes, and determined the fusion index and myosin heavy chain-positive area in myotubes. The contribution of HIF1α was studied by gain (CoCl2) and loss (siRNAs) of function experiments. We further examined hypoxia in LHCN-M2-iDUX4 myoblasts with inducible expression of DUX4, the transcription factor underlying FSHD pathology.

Results
We found that the hypoxic response did not impact myoblast proliferation but activated precocious myogenic differentiation and that HIF1α was critical for this process. Hypoxia also enhanced the late differentiation of human myocytes, but in an HIF1α-independent manner. Interestingly, the impact of hypoxia on muscle cell proliferation was influenced by dexamethasone. In the FSHD pathological context, DUX4 suppressed HIF1α-mediated precocious muscle differentiation.

Conclusion
Hypoxia stimulates myogenic differentiation in healthy myoblasts, with HIF1α-dependent early steps. In FSHD, DUX4-HIF1α interplay indicates a novel mechanism by which DUX4 could interfere with HIF1α function in the myogenic program and therefore with FSHD muscle performance and regeneration.
Angiotensin II treatment is associated with improved oxygenation in ARDS patients with refractory vasodilatory shock
Authors: Leisman Daniel E. , Handisides Damian R. , Chawla Lakhmir S. , Albertson Timothy E. , Busse Laurence W. , Boldt David W. , Deane Adam M. , Gong Michelle N. , Ham Kealy R. , Khanna Ashish K. , Ostermann Marlies , McCurdy Michael T. , Thompson B. Taylor , Tumlin James S. , Adams Christopher D. , Hodges Tony N. , Bellomo Rinaldo 
Published: December 2023

Abstract
Background
The physiological effects of renin-angiotensin system modulation in acute respiratory distress syndrome (ARDS) remain controversial and have not been investigated in randomized trials. We sought to determine whether angiotensin-II treatment is associated with improved oxygenation in shock-associated ARDS.

Methods
Post-hoc subgroup analysis of the Angiotensin Therapy for High Output Shock (ATHOS-3) trial. We studied patients who met modified Berlin ARDS criteria at enrollment. The primary outcome was PaO2/FiO2-ratio (P:F) at 48-h adjusted for baseline P:F. Secondary outcomes included oxygenation index, ventilatory ratio, PEEP, minute-ventilation, hemodynamic measures, patients alive and ventilator-free by day-7, and mortality.

Results
Of 81 ARDS patients, 34 (42%) and 47 (58%) were randomized to angiotensin-II or placebo, respectively. In angiotensin-II patients, mean P:F increased from 155 mmHg (SD: 69) at baseline to 265 mmHg (SD: 160) at hour-48 compared with no change with placebo (148 mmHg (SD: 63) at baseline versus 164 mmHg (SD: 74) at hour-48)(baseline-adjusted difference: + 98.4 mmHg [95%CI 35.2–161.5], p = 0.0028). Similarly, oxygenation index decreased by − 6.0 cmH2O/mmHg at hour-48 with angiotensin-II versus − 0.4 cmH2O/mmHg with placebo (baseline-adjusted difference: -4.8 cmH2O/mmHg, [95%CI − 8.6 to − 1.1], p = 0.0273). There was no difference in PEEP, minute ventilation, or ventilatory ratio. Twenty-two (64.7%) angiotensin-II patients had sustained hemodynamic response to treatment at hour-3 versus 17 (36.2%) placebo patients (absolute risk-difference: 28.5% [95%CI 6.5–47.0%], p = 0.0120). At day-7, 7/34 (20.6%) angiotensin-II patients were alive and ventilator-free versus 5/47(10.6%) placebo patients. Day-28 mortality was 55.9% in the angiotensin-II group versus 68.1% in the placebo group.

Conclusions
In post-hoc analysis of the ATHOS-3 trial, angiotensin-II was associated with improved oxygenation versus placebo among patients with ARDS and catecholamine-refractory vasodilatory shock. These findings provide a physiologic rationale for trials of angiotensin-II as treatment for ARDS with vasodilatory shock.
Trial Registration: ClinicalTrials.Gov Identifier: NCT02338843 (Registered January 14th 2015).
International relations challenges and sustainable development in developing countries after 2022: conceptualization of the risk assessment model
Authors: Stevanović Miroslav , Pavlićević Predrag , Vujinović Nikola , Radovanović Mirjana 
Published: December 2023

Abstract
Background
UN defined Transforming our world: the 2030 Agenda for Sustainable Development promotes international relations as a platform of cooperation between all stakeholders to achieve peace, stability, and prosperity but faces significant challenges in the post-2022 period. In light of the COVID-19 pandemic and the Ukraine crisis, the prospects for sustainable development as a component of international relations are one of the challenges. The main objective of this paper is the conceptualization of a model for sustainable development risk analysis, intended for developing countries with underdeveloped or nonexistent monitoring systems.

Methods
Theory synthesis, adaption, and model development were used in the conceptual method due to empirical research limitations. Analysis, induction, and modeling were utilized to evaluate qualitative and quantitative sustainable development risk assessment approaches and anticipate their advantages and disadvantages.

Results
The theoretical part of the research points to the need to consider the risks to implementing sustainable development (primarily future energy development and climate change) that arise from changes in international security. The novel result of the paper is the proposal of the concept Model for sustainable development implementation risk assessment in developing countries, derived from the following inputs: (a) an overview of the development of international relations and attitudes towards sustainable development; (b) a proposal of a generic model for risk assessment; (c) a prediction of the sustainable development future from the perspective of international relations and defined risks for its implementation. A set of indicators is proposed by dimensions: social, economic–political, and energy-environment.

Conclusions
The future of sustainable development can be considered at a certain degree of risk in light of the global crisis after 2022. Changes in geopolitical constellations, armed conflicts, population growth, climate change, and the reallocation of financial resources are the factors that will largely determine the commitment of individual countries to implementing sustainable development. Therefore, sustainable development risk assessment must be revisited, improved, and closely monitored. The development of models based on the specificities of each country is highly recommended. Methodological notes are presented.
Reduction of ammonia gas by microbial agent treatment in Chinese cabbage cultivation
Authors: Park Jong-Hwan , Lee Su-Lim , Lee Jae-Hoon , Rho Jun-Suk , Lee Jeong-Min , Kim Seong-Heon , Kang Se-Won , Seo Dong-Cheol 
Published: December 2023

AbstractThis study aimed to select the optimal microbial agents for ammonia gas reduction in Chinese cabbage cultivation and evaluate their ammonia reduction efficiency. By selecting the optimum microorganism to reduce ammonia emissions, the ammonia emission reduction efficiencies of the nitrification microorganisms, Alcaligenes faecalis subsp. faecalis and Brevibacillus sp. were 21 and 31%, respectively, which were superior to those of other microorganisms. The best ammonia emission reduction efficiency of the acid-producing microorganisms was 55%. The optimum mixing ratio of microbial agent for removing ammonia gas emitted from NPK-containing soil was: acid-producing microorganism:Alcaligenes faecalis subsp. faecalis:Brevibaillus sp. = 0.70:0.15:0.15. The optimum treatment amount was 500 L/ha, and the optimum number of microbial agents was basal fertilization (also known as pre-planting fertilization) once and additional fertilization three times, for a total of four times. The reduction efficiency of ammonia emissions from NPK-containing soil under optimum conditions in cabbage cultivation was 27% lower than that of the control (only NPK-containing soil). Therefore, the microbial agent developed in this study can be utilized to effectively reduce the emission of ammonia, a secondary fine particle precursor, while maintaining crop yield in agricultural fields.
Improved oxygenation in prone positioning of mechanically ventilated patients with COVID-19 acute respiratory distress syndrome is associated with decreased pulmonary shunt fraction: a prospective multicenter study
Authors: Harbut Piotr , Campoccia Jalde Francesca , Dahlberg Martin , Forsgren Anders , Andersson Elisabeth , Lundholm Andreas , Janc Jaroslaw , Lesnik Patrycja , Suchanski Michal , Zatorski Pawel , Trzebicki Janusz , Skalec Tomasz , Günther Mattias 
Published: December 2023

Abstract
Background
Prone position is used in acute respiratory distress syndrome and in coronavirus disease 2019 (Covid-19) acute respiratory distress syndrome (ARDS). However, physiological mechanisms remain unclear. The aim of this study was to determine whether improved oxygenation was related to pulmonary shunt fraction (Q’s/Q’t), alveolar dead space (Vd/Vtalv) and ventilation/perfusion mismatch (V’A/Q’).

Methods
This was an international, prospective, observational, multicenter, cohort study, including six intensive care units in Sweden and Poland and 71 mechanically ventilated adult patients.

Results
Prone position increased PaO2:FiO2 after 30 min, by 78% (83–148 mm Hg). The effect persisted 120 min after return to supine (p < 0.001). The oxygenation index decreased 30 min after prone positioning by 43% (21–12 units). Q’s/Q’t decreased already after 30 min in the prone position by 17% (0.41–0.34). The effect persisted 120 min after return to supine (p < 0.005). Q’s/Q’t and PaO2:FiO2 were correlated both in prone (Beta -137) (p < 0.001) and in the supine position (Beta -270) (p < 0.001). V’A/Q’ was unaffected and did not correlate to PaO2:FiO2 (p = 0.8). Vd/Vtalv increased at 120 min by 11% (0.55–0.61) (p < 0.05) and did not correlate to PaO2:FiO2 (p = 0.3). The ventilatory ratio increased after 30 min in the prone position by 58% (1.9–3.0) (p < 0.001). PaO2:FiO2 at baseline predicted PaO2:FiO2 at 30 min after proning (Beta 1.3) (p < 0.001).

Conclusions
Improved oxygenation by prone positioning in COVID-19 ARDS patients was primarily associated with a decrease in pulmonary shunt fraction. Dead space remained high and the global V’A/Q’ measure could not explain the differences in gas exchange.