bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of STAT3 alleviates LPS-induced apoptosis and inflammation in renal tubular epithelial cells by transcriptionally down-regulating TASL
Authors: Xu Jin-Wen , Wang Ming-Yan , Mao Yan , Hu Zheng-Yun , Miao Xiao-Lin , Jiang Feng , Zhou Guo-Ping 
Published: January 2024

Abstract
Background
Systemic lupus erythematosus (SLE) is a common autoimmune disease that impacts various organs. Lupus nephritis (LN) significantly contributes to death in children with SLE. Toll-like receptor (TLR) adaptor interacting with SLC15A4 on the lysosome (TASL) acts as an innate immune adaptor for TLR and is implicated in the pathogenesis of SLE. A transcription factor known as signal transducer and activator of transcription 3 (STAT3), which is known to be linked to autoimmune diseases, is also involved in the development of SLE.

Methods
Bioinformatics and real-time quantitative PCR (qRT-PCR) was used to detect the expression of STAT3 and TASL in peripheral blood of SLE patients and their correlation. Bioinformatics analysis, qRT-PCR, luciferase assay and chromatin immunoprecipitation (ChIP) were used to verify the regulation of transcription factor STAT3 on TASL. The expression levels of STAT3, TASL and apoptosis-related genes in LPS-induced HK2 cells were detected by qRT-PCR and Western blot. TUNEL staining were used to detect the apoptosis of HK2 cells after LPS stimulation. ELISA and qRT-PCR were used to detect the levels of inflammatory cytokines in the cell culture supernatant. TASL knockdown in HK2 cells was used to detect the changes in apoptosis-related genes and inflammatory factors. The expression level of TASL in LPS-stimulated HK2 cells and its effect on cell apoptosis and inflammatory factors were observed by knocking down and overexpressing STAT3, respectively. It was also verified in a rescue experiment.

Results
The expressions of STAT3 and TASL were higher in SLE than in healthy children, and the expression of STAT3 was positively correlated with TASL. Transcription factor STAT3 can directly and positively regulate the expression of TASL through the promoter region binding site. The expression of STAT3, TASL and inflammatory cytokines was elevated, and the change of apoptosis was up-regulated in LPS-stimulated HK2 cells. Inhibition of STAT3 alleviates LPS-stimulated apoptosis and inflammatory response in HK2 cells through transcriptional regulation of TASL.

Conclusions
These findings provide new insights into the transcriptional regulation of TASL and provide new evidence of a direct regulatory relationship between signaling nodes in the lupus signaling network.

Graphical Abstract
Association of body temperature and mortality in critically ill patients: an observational study using two large databases
Authors: Tan Daniel J. , Chen Jiayang , Zhou Yirui , Ong Jaryl Shen Quan , Sin Richmond Jing Xuan , Bui Thach V. , Mehta Anokhi Amit , Feng Mengling , See Kay Choong 
Published: January 2024

Abstract
Background
Body temperature (BT) is routinely measured and can be controlled in critical care settings. BT can impact patient outcome, but the relationship between BT and mortality has not been well-established.

Methods
A retrospective cohort study was conducted based on the MIMIC-IV (N = 43,537) and eICU (N = 75,184) datasets. The primary outcome and exposure variables were hospital mortality and first 48-h median BT, respectively. Generalized additive models were used to model the associations between exposures and outcomes, while adjusting for patient age, sex, APS-III, SOFA, and Charlson comorbidity scores, temperature gap, as well as ventilation, vasopressor, steroids, and dialysis usage. We conducted subgroup analysis according to ICU setting, diagnoses, and demographics.

Results
Optimal BT was 37 °C for the general ICU and subgroup populations. A 10% increase in the proportion of time that BT was within the 36–38 °C range was associated with reduced hospital mortality risk in both MIMIC-IV (OR 0.91; 95% CI 0.90–0.93) and eICU (OR 0.86; 95% CI 0.85–0.87). On the other hand, a 10% increase in the proportion of time when BT < 36 °C was associated with increased mortality risk in both MIMIC-IV (OR 1.08; 95% CI 1.06–1.10) and eICU (OR 1.18; 95% CI 1.16–1.19). Similarly, a 10% increase in the proportion of time when BT > 38 °C was associated with increased mortality risk in both MIMIC-IV (OR 1.09; 95% CI 1.07–1.12) and eICU (OR 1.09; 95% CI 1.08–1.11). All patient subgroups tested consistently showed an optimal temperature within the 36–38 °C range.

Conclusions
A BT of 37 °C is associated with the lowest mortality risk among ICU patients. Further studies to explore the causal relationship between the optimal BT and mortality should be conducted and may help with establishing guidelines for active BT management in critical care settings.
ER-positive and BRCA2-mutated breast cancer: a literature review
Authors: Li Pu-Chun , Zhu Yi-Fan , Cao Wen-Ming , Li Bei 
Published: January 2024

AbstractBRCA2-mutated carriers have a high lifetime risk of breast cancer (BC), an early age of onset, and an increased risk of other cancers (including ovarian, pancreatic, and prostate cancer). Almost 70–80% of BRCA2-mutated BC are estrogen receptor (ER)-positive, which is a particular type of ER-positive BC that differs from sporadic ER-positive BC. This article reviews the clinicopathological features, treatment, and prognosis of ER-positive and BRCA2-mutated BC to provide a reference for clinical decision-making.
Optimizing cardiac amyloidosis assessment: utility of 1-h and 3-h 99mTc-PYP imaging
Authors: Imakhanova Aiganym , Ideguchi Reiko , Kawano Hiroaki , Maemura Koji , Kudo Takashi 
Published: January 2024

Abstract
Background
Transthyretin amyloid cardiomyopathy (ATTR-CM), characterized by the extracellular deposition of an insoluble amyloid protein in the heart, is one of the main causes of heart failure in elderly patients. In this study, our primary objective was to explore the diverse applications and temporal significance of 1-h and 3-h imaging using 99mTc-PYP in the context of ATTR-CM. Additionally, we compared tracer kinetics in the heart and bone to comprehensively assess the diagnostic advantages and time-related considerations associated with these two incubation periods.

Methods
Twenty-seven patients at Nagasaki University Hospital who underwent 99mTc-PYP planar, and SPECT cardiac imaging were classified into two groups (ATTR-CM-positive and -negative groups) based on the American Heart Association statement. Cardiac retention was assessed with both a semiquantitative visual score and a quantitative analysis. To assess bone accumulation, a ROI with an equal volume was drawn on the sternum and calculated as the bone-to-contralateral ratio (B/CL). We also evaluated correlation between heart-to-contralateral lung (H/CL) ratio and left ventricular wall thickness.

Results
Among patients who underwent 99mTc-PYP imaging, the H/CL ratio was significantly higher at 1 h than at 3 h regardless of the group (from 2.20 ± 0.36 to 1.99 ± 0.35, p < 0.01 in the positive group and from 1.35 ± 0.12 to 1.19 ± 0.21, p = 0.01 in the negative group). The gap of H/CL between highest H/CL of negative case and lowest H/CL of positive case was narrower in 3 h. On the other hand, correlation between H/CL and left ventricular posterior wall thickness tends to be clearer in 3 h (p = 0.12, r = 0.30 for 1 h, p = 0.04, r = 0.39 at 3 h).

Conclusion
Our study suggests that both 1-h and 3-h incubation times for 99mTc-PYP imaging have different benefits for ATTR cardiac amyloidosis. A one-hour incubation may be preferable for differential diagnostic purposes, while a three-hour incubation may provide greater utility in evaluating disease severity.
Structuring the complexity of drop-out from VET: a theoretical framework guiding empirical research perspectives
Authors: Krötz Maximilian 
Published: January 2024

AbstractAfter more than 50 years of research on drop-out from Vocational Education and Training (VET), databases provide vast amounts of drop-out studies from around the globe that entail hundreds of potential drop-out factors. However, many scholars tend to explore the topic without detailed and theoretically grounded foundation. Several diverging research foci exist within the research field of drop-out from VET: Studies differ with regard to the analysed phase of drop-out (development, decision-making, paths after dropping out), the applied sample perspective (learners, educators, further stakeholders), and the overall research perspective that is applied based on a specific theoretical approach. Given the range of drop-out factors, stakeholders, and possible theoretical approaches within studies, this article aims at organising the research on the multi-facetted phenomenon for future endeavours by presenting a framework model that comprises a three-step recommendation: First, the model illustrates different phases (Development-, Decision-, Adjustment-Phase) of drop-out research that should be differentiated. Second, it is argued that the chosen sample perspective is highly relevant for drop-out research and must be chosen carefully. Third, the overarching disciplinary research perspectives on drop-out from VET, which scholars may adopt within their research, entail particular relevant variables and focal points and should therefore be discussed. Four examples of research perspectives (economical, psychological, sociological, pedagogical) are briefly presented, structured on a micro-, meso- and macro-level, to exemplify different theoretical accesses to drop-out research. Throughout the article, the novel organisation framework is explained and exemplified by illustrating relevant aspects of each research area and exemplary literature. The presented framework will help organising the research field and enable scholars to delimit their work more precisely, leading to a more traceable structure of further research efforts and more significant contributions to the state of knowledge.
Does teachers’ motivation have an impact on students’ scientific literacy and motivation? An empirical study in Colombia with data from PISA 2015
Authors: Suárez-Mesa Ana María , Gómez Ricardo L. 
Published: January 2024

AbstractIn this study we use data from the Programme for International Student Assessment (PISA) to investigate the effect of teachers’ motivation on students’ scientific literacy and motivation in Colombia. These relationships are explored using a multilevel modeling framework and through the lens of Self-Determination Theory. Although difficulties in achieving education quality in developing and emerging economies are commonly attributed to teacher motivation issues, and important policy measures are implemented based on this assumption, this topic remains largely empirically unexplored. The purpose of the study is to contribute to fill this gap and provide empirically based insights for a broader and more informed dialogue regarding the effect of motivation in the development of scientific literacy, and to the design and implementation of evidence-based policies, instructional practices, and interventions. In this analysis, we did not find a significant relationship between teacher motivation and either students’ scientific literacy or motivation. However, students’ interest in science and sense of self-efficacy were significantly associated with their own achievement. The results also show that teacher-directed instruction is the strongest predictor of scientific literacy as opposed to inquiry-based teaching. However, inquiry-based teaching was found to be a positive predictor of increased students’ motivation.
#AidToo, or when situation permits rape: sexual violence among humanitarian aid workers
Authors: Sauter Melanie 
Published: January 2024

AbstractThis article explores the prevalent issue of sexual violence against and among humanitarian aid workers, with a focus on why the aid sector creates a particularly permissive environment for sexual violence. Although the issue is primarily an industry problem, with both perpetrators and survivors being members of the aid community, humanitarian organizations have been hesitant to address the issue. The article argues that legal ambiguities, barriers to accessing justice, and organizational mismanagement contribute to the permissive environment in the aid industry. Drawing on secondary survivor testimonies and legal frameworks, I highlight the need for more tangible prevention and follow-up mechanisms for survivors based on the legal duty of care of aid organizations. The analysis underscores the need for a comprehensive and proactive approach to addressing sexual violence within the aid sector, grounded in an understanding of power dynamics and organizational culture.
Kvaal’s dental age estimation method applied to Brazilian adults—a cross-sectional test of validity
Authors: Mantovani Suzana , Petta Adriano , Arakelyan Marianna , Soares Mariana , Franco Ademir 
Published: January 2024

Abstract
Background
Dental age estimation of adults is a challenging procedure in forensic odontology. Radiographic techniques have enabled non-invasive age estimation of adults since the 90’s. Kvaal’s method based on the deposition of secondary dentin has demonstrated broad applicability in several populations. Population-specific studies with this method, however, are scarce in South America. This study tested the validity of Kvaal’s method in a sample of radiographs of Brazilian adults. The sample consisted of 192 periapical radiographs of 106 females (55.2%) and 86 males (44.8%) with ages between 20 and 80 years old. The permanent maxillary right (n = 84, 43.8%) and left (n = 108, 56.2%) central incisors were assessed based on Kvaal’s method.

Results
Lin’s coefficient of concordance between estimated (EA) and chronological (CA) ages was 0.800 (IC95% = 0.753; 0.847) (p < 0.001). The mean difference between EA and CA was − 2.84 years (p < 0.001). For females and males, separately, the mean differences between EA and CA were − 3.82 years (CI95% =  − 5.38; − 2.25) (p < 0.001) and − 1.86 years (CI95% =  − 3.56; − 0.17) (p = 0.031), respectively. Underestimations were predominant and increased with time.

Conclusion
After 28 years of the original publication, Kvaal’s method remains reliable for non-invasive dental age estimation of adults, especially for the analysis of the permanent maxillary central incisors of Brazilian individuals.
Comparison of nutritional values of Mucuna pruriens L. (velvet bean) seeds with the most preferred legume pulses
Authors: Boniface Florence , Washa Washa B. , Nnungu Stephen 
Published: January 2024

AbstractThe effectiveness of legumes in overcoming hunger and food insecurity is attributed to their accessibility. They have been recorgnised for their nutritional significance and their ability to provide food stability in tropical and sub-tropical regions. This study compared the nutritional values of Mucuna seeds with that of common legume pulses by analysing their percentage composition based on literature review. Similar to common legume pulses, Mucuna seeds have been found to contain promising nutritional value. However, unlike most preferred legume pulses, Mucuna seeds contain a notable quantity of anti-nutritional factors that interferes with its nutritional qualities. Besides being anti-nutritional, the compounds have bio-active potentials and have been associated with therapeutic and antioxidant activities. Notably, Mucuna pruriens L. is known to contain compounds with potential antiparkinsonian effects, such as L-Dopa and ursolic acid. Considering their high productivity and nutritional relevance, Mucuna seeds have been utilised as traditional foods in populations with lower incomes that suffer from chronic undernourishment. It should be noted that variations in agro-climatic conditions have been reported to impact the chemical composition of M. pruriens seeds. However, limited information on the chemical composition of M. pruriens seeds from different regions makes it challenging to compare their composition across various agro-climates. Furthermore, in order to support the widespread use of M. pruriens in different areas, further research is needed to determine the optimal conditions for cultivating highly nutritious, phytochemically rich, and commercially viable seeds. Additionally, it is important to evaluate the effectiveness of L-Dopa in treating Parkinsonian patients across a diverse range of populations.
Graphical Abstract
Associations Between Adiponectin and the Development of Diabetes in Rheumatoid Arthritis
Authors: Baker Joshua F , England Bryant R , Wysham Katherine D , Sauer Brian , Joseph Amy M , Lenert Aleksander , Roul Punyasha , Xiao Rui , Gillcrist Rachel , Johnson Tate , Cannon Grant W , Duryee Michael , Thiele Geoffrey M , Mikuls Ted R 
Published: January 2024

Abstract

Purpose
We evaluated associations between adiponectin and the risk of diabetes among patients with rheumatoid arthritis (RA), a systemic inflammatory disease associated with metabolic disturbance.

Methods
This prospective cohort study included adults with RA from the Veterans Affairs Rheumatoid Arthritis Registry. Adiponectin and inflammatory cytokines/chemokines were measured at enrollment on stored serum samples. Adiponectin levels were categorized, and clinical variables were described across categories (&lt;10 μg/mL; 10-40 μg/mL; &gt;40 μg/mL). Multivariable Cox proportional hazard models evaluated associations between adiponectin and incident diabetes adjusting for age, sex, race, smoking status, body mass index (BMI), disease-modifying therapy use, calendar year, and comorbidity. Testing for modification of effect in the context of elevated cytokines/chemokines was performed.

Results
Among 2595 patients included in the analysis, those with adiponectin levels &gt;40 μg/mL (N = 379; 15%) were older and had lower BMI. There were 125 new cases of diabetes among 1689 patients without prevalent disease at enrollment. There was an inverse association between adiponectin and incident diabetes; however, the association was positive among patients with adiponectin levels &gt;40 μg/mL. Patients with levels &gt;40 μg/mL were at higher risk compared to those with levels 10-40 μg/mL (HR: 1.70 [1.34, 2.16] P &lt; .001). Those with adiponectin levels &gt;40 μg/mL had significantly higher levels of inflammatory cytokines with evidence of a modified effect of adiponectin on diabetes risk in the setting of inflammation.

Conclusion
The relationship between adiponectin and incident diabetes risk is U-shaped in RA. Patients with very high adiponectin levels have greater systemic inflammation and an altered relationship between adiponectin and diabetes risk.
Acne vulgaris - review on pathogenesis and treatment
Authors: Żmuda Bartłomiej , Żuberek Michał , Ślusarczyk Daniel , Pisera Piotr , Kiełkowicz Aleksandra , Popińska Zuzanna , Pactwa Filip , Jakubowska Wiktoria 
Published: January 2024

Introduction:
Acne vulgaris is a common dermatological problem, resulting from a complex interaction of many factors. This article presents a comprehensive overview of the pathogenesis of this condition, examining key aspects such as inflammatory factors, sebum production, colonization by P. acnes, endocrine disruption and lifestyle influences. In addition, various treatments are discussed, including the effectiveness of isotretinoin-based therapy and the potential benefits of antibiotics and retinoids in specific patient groups.
Aim of study:
The aim of the study is to summarize the available knowledge about the Acne vulgaris. The epidemiology, etiology, and methods of treatment were summarized and described.
Materials and methods:
The literature available in PubMed database was reviewed using following keywords: “Acne vulgaris”, “Isotretinoin”, “Therapy of Acne”.
Conclusion:
Research into acne vulgaris has been extensive, yet variations in study methods and descriptions have prevented definitive conclusions. Tailored care for each patient is crucial due to these differences. The disease's development involves various factors including inflammatory mediators, excess sebum production, P. acnes colonization, hormonal imbalances, and lifestyle impacts. Isotretinoin remains the most effective treatment, albeit with potential side effects that need consideration. For specific patient groups, topical antibiotics and retinoids may offer beneficial effects.
Menetrier's disease - press review and summary of current knowledge
Authors: Markowiak Szymon , Wardęszkiewicz Marta , Jabłońska Wiktoria , Kasprzak Amelia , Świercz Maciej , Truchta Monika , Mańkowska Aleksandra , Kolano Agata , Pejas Anna 
Published: January 2024

Introduction
Menetrier's Disease is a rare and mysterious stomach disorder characterised by giant gastric folds, excessive mucus production, and protein loss. This comprehensive research article examines Menetrier's Disease, focusing on its clinical features, diagnosis, and available treatment options. The article aims to provide a complete understanding of Menetrier's Disease, fill the knowledge gap, and encourage improved clinical management.
Description of the State of Knowledge
Menetrier's Disease is a rare condition, and its cause is still unknown, though it may be linked to previous viral and bacterial infections and increased expression of transforming growth factor-alpha (TGF-α). Diagnosis can be established by endoscopic examination and histological analysis of material from a full-thickness mucosal biopsy. Treatment options are limited and concentrated on symptom relief, while the outcome of the therapy may vary.
Summary
Menetrier's Disease presents significant difficulties in both diagnosis and treatment. This review outlines the clinical features, classification, diagnostic approaches, available treatment options, and prognosis for healthcare professionals and researchers seeking to understand this disease better. Better understanding of Menetrier's Disease is crucial to improve patient care and explore new, potential therapeutic avenues.
Nanostructured p-PbS/p-CuO sulfide/oxide bilayer heterojunction as a promising photoelectrode for hydrogen gas generation
Authors: Zayed Mohamed , Aljaafreh Mamduh J. , Shaban Mohamed , Hezam Mahmoud , Mohamed Sodky H. , Rabia Mohamed , Marashdeh Mohammad , Al-Hmoud M. , Ahmed Ashour M. 
Published: January 2024

Abstract
Recently, the photoelectrochemical (PEC) water-splitting reaction for hydrogen (H2) production has been a competitive research route to realize clean and sustainable electric power. In this work, copper oxide (CuO) and PbS thin films were fabricated on commercial glass, respectively, using the techniques of successive ionic-layer adsorption and reaction and chemical bath deposition. These nanostructured thin films served successfully as photoelectrodes for the photogeneration of H2. In addition, a p-PbS/p-CuO bilayer system was also fabricated, and a remarkable boost in PEC efficiency was observed compared to pure CuO and PbS thin films. Optical examinations showed excellent absorbance properties of the p-PbS/p-CuO bilayer in the visible range, with a bandgap of ∼1.28 eV. X-ray diffraction analysis indicated a monoclinic CuO/cubic PbS crystalline structure with a particle size of ∼18 nm. The photocurrent density (J
ph) values were obtained using a three-electrode electrochemical cell in 0.3 M Na2S2O3 electrolyte. The p-PbS/p-CuO photoelectrode demonstrated a J
ph value of −0.390 mA cm−2, which is significantly higher than the values of −0.120 and −0.008 mA cm−2 for the pure PbS and CuO photoelectrodes, respectively. This improvement is attributed to the p-PbS/p-CuO oxide/sulfide bilayer heterojunction, which improved the visible light absorption and reduced the electron–hole (e–h) recombination. The effects of pH value, temperature light intensity, and wavelength were all additionally studied. Remarkably, the photoelectrodes were stable under a pH of ∼7, which makes them promising for H2 production using normal drinking/seawater. These findings confirm the ability of the prepared photoelectrodes to facilitate water splitting and H2 generation under various environmental, chemical, and illumination conditions.