bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA degradation in Haplaxius crudus (Hemiptera: Cixiidae) and Diaphorina citri (Hemiptera: Liviidae) on yellow sticky traps in Florida
Authors: Bloch Melody , Mou De-Fen , Helmick Ericka E , Pelz-Stelinski Kirsten , Bahder Brian W 
Published: October 2023

Abstract
Lethal bronzing (LB) and huanglongbing (HLB) are harmful plant diseases causing significant economic losses in Florida agriculture. Both diseases are caused by bacteria that are transmitted by Hemipteran insect vectors. Accurate detection of pathogens within insect vectors can help provide a better understanding of disease epidemiology. Monitoring of the vector of LB is done primarily using sticky traps within palm canopies. However, it is unknown how long pathogen and vector DNA remain intact under field conditions. If significant DNA degradation takes place over the course of days or weeks, there is a possibility of false negatives occurring when detecting pathogens from these surveys. This study determined how long Haplaxius crudus Van Duzee (Hemiptera: Cixiidae) and LB DNA could remain detectable on sticky traps under field conditions in Florida in winter and summer, using PCR and qPCR. Additionally, this study compared the DNA degradation of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. The results showed that DNA concentration and amplification rate declined as time on sticky traps increased. Degradation varied between different target genes. The amplification rate of insect genes from sticky trap samples suggests that sticky traps should be changed weekly in summer, and every 2 wk in winter for accurate H. crudus detection. Traps should be changed every 4 days for phytoplasma detection. Traps can be changed monthly for accurate D. citri and CLas detection. Based on these results, standard monitoring protocols can be implemented to more accurately detect vectors and pathogens.
CD6 triggers actomyosin cytoskeleton remodeling after binding to its receptor complex
Authors: Borjini Nozha , Lun Yu , Jang Geen-Fu , Crabb Jack , Chen Yinghua , Crabb John , Fox David A , Ivanov Andrei I , Lin Feng 
Published: October 2023

Abstract
The T cell marker CD6 regulates both T cells and target cells during inflammatory responses by interacting with its receptors. However, only a few receptors binding to the extracellular domains of CD6 have been identified, and cellular events induced by CD6 engagement with its receptors in target cells remain poorly understood. In this study, we identified CD44 as a novel CD6 receptor by proximity labeling and confirmed the new CD6-CD44 interaction by biochemical and biophysical approaches. CD44 and the other 2 known CD6 receptors, CD166 and CDCP1, were distributed diffusely on resting retinal pigment epithelium (RPE) cells but clustered together to form a receptor complex upon CD6 binding. CD6 stimulation induced dramatic remodeling of the actomyosin cytoskeleton in RPE cells mediated by activation of RhoA, and Rho-associated kinase signaling, resulting in increased myosin II phosphorylation. Such actomyosin activation triggered the disassembly of tight junctions responsible for RPE barrier integrity in a process that required all components of the tripartite CD6 receptor complex. These data provided new insights into the mechanisms by which CD6 mediates T cell–driven disruption of tissue barriers during inflammation.
Concentration and Competition: Evidence From Europe and Implications For Policy
Authors: Koltay Gábor , Lorincz Szabolcs , Valletti Tommaso 
Published: October 2023

Abstract
The paper provides new evidence on proxy indicators of market power for major European countries. The data show moderately increasing average industry concentration over the last two decades, a considerably increasing proportion of high-concentration industries, and an overall tendency toward oligopolistic structure. Estimates of aggregate profitability also show a sustained increase over the recent decades for European economies. Although the academic and policy debate is not settled as to whether the causes of these trends are policy driven or reflect technological improvement, our findings suggest that competition policy is likely to face more challenges as large companies are becoming more common in more and more industries.
A national survey on the medical physics workload of external beam radiotherapy in Japan
Authors: Tohyama Naoki , Okamoto Hiroyuki , Shimomura Kohei , Kurooka Masahiko , Kawamorita Ryu , Ota Seiichi , Kojima Toru , Hayashi Naoki , Okumura Masahiko , Nakamura Masaru , Nakamura Mitsuhiro , Myojoyama Atsushi , Onishi Hiroshi 
Published: October 2023

Abstract
Several staffing models are used to determine the required medical physics staffing, including radiotherapy technologists, of radiation oncology departments. However, since Japanese facilities tend to be smaller in scale than foreign ones, those models might not apply to Japan. Therefore, in this study, we surveyed workloads in Japan to estimate the optimal medical physics staffing in external beam radiotherapy. A total of 837 facilities were surveyed to collect information regarding radiotherapy techniques and medical physics specialists (RTMPs). The survey covered facility information, staffing, patient volume, equipment volume, workload and quality assurance (QA) status. Full-time equivalent (FTE) factors were estimated from the workload and compared with several models. Responses were received from 579 facilities (69.2%). The median annual patient volume was 369 at designated cancer care hospitals (DCCHs) and 252 across all facilities. In addition, the median FTE of RTMPs was 4.6 at DCCHs and 3.0 at all sites, and the average QA implementation rate for radiotherapy equipment was 69.4%. Furthermore, advanced treatment technologies have increased workloads, particularly in computed tomography simulations and treatment planning tasks. Compared to published models, larger facilities (over 500 annual patients) had a shortage of medical physics staff. In very small facilities (about 140 annual patients), the medical physics staffing requirement was estimated to be 0.5 FTE, implying that employing a full-time medical physicist would be inefficient. However, ensuring the quality of radiotherapy is an important issue, given the limited number of RTMPs. Our study provides insights into optimizing staffing and resource allocation in radiotherapy departments.
The Convent Camp: Sacred Places in Palestinian Refugee History
Authors: Goodgame Clayton 
Published: October 2023

AbstractIn 1948, thousands of Palestinians fled to the Old City of Jerusalem, where many Orthodox Christians were housed by the Patriarchate of Jerusalem. This article asks how Jerusalem refugee histories change when we consider the patriarchate’s characteristics as an informal camp. The religious dimensions of camps are often overlooked, and the article suggests that recognizing them affords a better understanding of the forms of historical consciousness that refugee camps foster. Drawing on memoirs of Palestinian Orthodox refugees and ethnography from contemporary Jerusalem, the article highlights the powerful role sacred sites have played in the preservation of refugee history since the war, and the effect they have on the political dynamics of the church and the Old City of Jerusalem.
Lightweight macroporous mesh can be used for corporal reconstruction during penile prosthesis surgery
Authors: Lee Wai Gin , Gordon Patrick , Ralph David 
Published: October 2023

Abstract

Background
Options to reinforce or reconstruct the corpora cavernosa during penile prosthesis surgery are limited. Synthetic grafts may confer a higher infection risk. Lightweight macroporous mesh is a promising alternative due to better integration and tissue ingrowth.

Aim
The study sought to report the first experience of using lightweight mesh to reinforce or reconstruct the corpora.

Methods
The medical records of all patients undergoing insertion or revision of penile prosthesis between May 2016 and May 2021 were reviewed retrospectively. Patient characteristics, management, and outcomes were extracted in which mesh was used for corporal reconstruction. Poliglecaprone-25/polypropylene mesh (UltraPro) was used in all cases. The surgical technique and alternatives were summarized.

Outcomes
The outcomes were infection rate, postoperative complications by Clavien-Dindo classification, and patient-reported outcomes.

Results
Mesh was required during penile prosthesis surgery in 21 men (median age 56 [range, 18-74] years). Reasons for reconstruction were severe corporal fibrosis (n = 6), impending erosion (n = 6), crossover/perforation (n = 6), congenital corporal agenesis (n = 2), and excision of Peyronie’s plaque. All but 2 patients (with corporal agenesis) presented for revision penile prosthesis surgery with a median number of previous revision attempts of 2 (range, 1-5). Two (10%) patients required explant after a mean follow-up of 23 ± 8.7 months. One man developed infection of the device 2 years after surgery, translating to an infection rate of 5% despite a high prevalence of diabetes (25%) in this cohort. Another required explantation due to debilitating chronic pain after 3 months. Further revision surgery for stiction syndrome was required in a third patient. Finally, another man was not satisfied with the position of the pump within the scrotum or the axial rigidity of the device, despite a second opinion suggesting no issues with the device. All men were sexually active following surgery.

Clinical implications
A lightweight mesh can be considered to reinforce or reconstruct the corpora cavernosa in complex cases in which there are no alternative techniques available.

Strengths and limitations
This is the first study using a lightweight macroporous mesh for revision penile prosthesis surgery. This was a well-characterized cohort of patients. A larger cohort with 5-year follow-up would be preferable.

Conclusion
These early results suggest that a lightweight macroporous mesh may be an acceptable synthetic graft for corporal reconstruction. Poliglecaprone-25/polypropylene mesh may be ideal because it is partially absorbable, easy to handle, and not bulky.
An X-rays-to-radio investigation of the nuclear polarization from the radio-galaxy Centaurus A
Authors: Marin Frédéric , Barnouin Thibault , Ehlert Steven R , Peirson Abel Lawrence , Lopez-Rodriguez Enrique , Petropoulou Maria , Wu Kinwah , Martí-Vidal Iván 
Published: October 2023

ABSTRACT
Centaurus A is one of the closest radio galaxies to Earth. Its proximity allowed us to extensively study its active galactic nucleus but the core emission mechanism remains elusive because of local strong dust and gas obscuration. The capability of polarimetry to shave-off contaminating emission has been exploited without success in the near-infrared by previous studies but the very recent measurement of the 2–8 keV polarization by the Imaging X-ray Polarimetry Explorer (IXPE) brought the question back to the fore. To determine what is the prevalent photon generation mechanism to the multiwavelength emission from the core of Centaurus A, we retrieved from the archives the panchromatic polarization measurements of the central compact component. We built the total and polarized flux spectral energy distributions of the core and demonstrated that synchrotron self-Compton models nicely fit the polarized flux from the radio to the X-ray band. The linear polarization of the synchrotron continuum is perpendicular to the jet radio axis from the optical to the radio band, and parallel to it at higher energies. The observed smooth rotation of the polarization angle in the ultraviolet band is attributed to synchrotron emission from regions that are getting closer to the particle acceleration site, where the orientation of the jet’s magnetic fields become perpendicular to the jet axis. This phenomenon support the shock acceleration mechanism for particle acceleration in Centaurus A, in line with IXPE observations of several high-synchrotron peak blazars.
Obscuration beyond the nucleus: infrared quasars can be buried in extreme compact starbursts
Authors: Andonie Carolina , Alexander David M , Greenwell Claire , Puglisi Annagrazia , Laloux Brivael , Alonso-Tetilla Alba V , Calistro Rivera Gabriela , Harrison Chris , Hickox Ryan C , Kaasinen Melanie , Lapi Andrea , López Iván E , Petter Grayson , Almeida Cristina Ramos , Rosario David J , Shankar Francesco , Villforth Carolin 
Published: October 2023

ABSTRACT
In the standard quasar model, the accretion disc obscuration is due to the canonical dusty torus. Here, we argue that a substantial part of the quasar obscuration can come from the interstellar medium (ISM) when the quasars are embedded in compact starbursts. We use an obscuration-unbiased sample of 578 infrared (IR) quasars at z ≈ 1–3 and archival Atacama Large Millimetre/submillimetre Array submillimetre host galaxy sizes to investigate the ISM contribution to the quasar obscuration. We calculate star formation rates (SFR) and ISM column densities for the IR quasars and a control sample of submillimetre galaxies (SMGs) not hosting quasar activity and show that: (1) the quasar obscured fraction is constant up to $\rm SFR\approx 300 \: {\rm M}_{\odot } \: yr^{-1}$, and then increases towards higher SFR, suggesting that the ISM obscuration plays a significant role in starburst host galaxies, and (2) at $\rm SFR\gtrsim 300 \: {\rm M}_{\odot } \: yr^{-1}$, the SMGs and IR quasars have similarly compact submillimetre sizes ($R_{\rm e}\approx 0.5{\!-\!}3\,\mathrm{ kpc}$) and consequently, the ISM can heavily obscure the quasar, even reaching Compton-thick ($N_{\rm H}\gt 10^{24} \rm \: cm^{-2}$) levels in extreme cases. Based on our results, we infer that ${\approx} 10{\!-\!}30~{{ \rm per\ cent}}$ of the IR quasars with $\rm SFR\gtrsim 300 \: {\rm M}_{\odot } \: yr^{-1}$ are obscured solely by the ISM.
Japan College of Rheumatology guidance for the use of methotrexate in patients with rheumatoid arthritis: Secondary publication
Authors: Kameda Hideto , Yamaoka Kunihiro , Yamanishi Yuji , Tada Masahiro , Koike Ryuji , Nakajima Ayako , Fusama Mie , Fujii Takao 
Published: October 2023

ABSTRACT
Methotrexate (MTX), the anchor drug in the current treatment strategy for rheumatoid arthritis (RA), was first approved for the treatment of RA in Japan in 1999 at a recommended dose of 6–8 mg/week. The approved maximum dose of MTX has been 16 mg/week since February 2011 when MTX was approved as a first-line drug in the treatment of RA. Recent evidence of MTX-polyglutamate concentration in the red blood cells of Japanese patients with RA justifies the current daily use of MTX in Japan. Additionally, after a nationwide clinical trial, a subcutaneous MTX injection formula (7.5–15 mg/week) was approved for RA treatment in September 2022. Therefore, in March 2023, a subcommittee of the Japan College of Rheumatology updated the guidance (formerly ‘guidelines’) for the use of MTX in Japanese patients with RA. This article, an abridged English translation summarizing the 2023 update of the Japan College of Rheumatology guidance for the use of MTX and management of patients with RA, will be helpful to both Japanese and global rheumatology communities.
Emerging roles of the CIP2A–TopBP1 complex in genome integrity
Authors: Ummethum Henning , Li Jiayi , Lisby Michael , Oestergaard Vibe H 
Published: October 2023

Abstract
CIP2A is an inhibitor of the tumour suppressor protein phosphatase 2A. Recently, CIP2A was identified as a synthetic lethal interactor of BRCA1 and BRCA2 and a driver of basal-like breast cancers. In addition, a joint role of TopBP1 (topoisomerase IIβ-binding protein 1) and CIP2A for maintaining genome integrity during mitosis was discovered. TopBP1 has multiple functions as it is a scaffold for proteins involved in DNA replication, transcriptional regulation, cell cycle regulation and DNA repair. Here, we briefly review details of the CIP2A–TopBP1 interaction, its role in maintaining genome integrity, its involvement in cancer and its potential as a therapeutic target.
Analysis of Plant-Specific ANTH Domain–Containing Protein in Marchantia polymorpha
Authors: Minamino Naoki , Fujii Haruki , Murata Haruhiko , Hachinoda Sho , Kondo Yohei , Hotta Kazuhiro , Ueda Takashi 
Published: October 2023

Abstract
Membrane trafficking is a fundamental mechanism for protein and lipid transport in eukaryotic cells and exhibits marked diversity among eukaryotic lineages with distinctive body plans and lifestyles. Diversification of the membrane trafficking system is associated with the expansion and secondary loss of key machinery components, including RAB GTPases, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and adaptor proteins, during plant evolution. The number of AP180 N-terminal homology (ANTH) proteins, an adaptor family that regulates vesicle formation and cargo sorting during clathrin-mediated endocytosis, increases during plant evolution. In the genome of Arabidopsis thaliana, 18 genes for ANTH proteins have been identified, a higher number than that in yeast and animals, suggesting a distinctive diversification of ANTH proteins. Conversely, the liverwort Marchantia polymorpha possesses a simpler repertoire; only two genes encoding canonical ANTH proteins have been identified in its genome. Intriguingly, a non-canonical ANTH protein is encoded in the genome of M. polymorpha, which also harbors a putative kinase domain. Similar proteins have been detected in sporadic lineages of plants, suggesting their ancient origin and multiple secondary losses during evolution. We named this unique ANTH group phosphatidylinositol-binding clathrin assembly protein-K (PICALM-K) and characterized it in M. polymorpha using genetic, cell biology–based and artificial intelligence (AI)–based approaches. Our results indicate a flagella-related function of MpPICALM-K in spermatozoids, which is distinct from that of canonical ANTH proteins. Therefore, ANTH proteins have undergone significant functional diversification during evolution, and PICALM-K represents a plant-unique ANTH protein that is delivered by neofunctionalization through exon shuffling.
PEP-ASSOCIATED PROTEIN 3 regulates rice tiller formation and grain yield by controlling chloroplast biogenesis
Authors: Seo Deok Hyun , Jang Jinwoo , Park Dongryeol , Yoon Youngdae , Choi Yang Do , Jang Geupil 
Published: October 2023

Abstract
Plastid-encoded RNA polymerase (PEP) plays a pivotal role in chloroplast development by governing the transcription of chloroplast genes, and PEP-associated proteins (PAPs) modulate PEP transcriptional activity. Therefore, PAPs provide an intriguing target for those efforts to improve yield, by enhancing chloroplast development. In this study, we identified the rice (Oryza sativa) OsPAP3 gene and characterized its function in chloroplast development. OsPAP3 expression was light-dependent and leaf-specific, similar to the PEP-dependent chloroplast gene RUBISCO LARGE SUBUNIT (OsRbcL), and OsPAP3 protein localized to chloroplast nucleoids where PEP functions. Analysis of loss-of-function and gain-of-function mutants showed that the expression of OsPAP3 is tightly linked to chloroplast gene expression and chloroplast biogenesis in rice. Homozygous knockout mutants of OsPAP3 had fewer chloroplasts than wild type, whereas plants overexpressing OsPAP3 had more chloroplasts. Also, OsPAP3 knockout suppressed the PEP-dependent expression of chloroplast genes, but OsPAP3 overexpression increased their expression. These findings indicate that OsPAP3 regulates chloroplast biogenesis in rice by controlling the PEP-dependent expression of chloroplast genes. More importantly, data from 3 seasons of field cultivation revealed that the overexpression of OsPAP3 improves rice grain yield by approximately 25%, largely due to increased tiller formation. Collectively, these observations suggest that OsPAP3 regulates rice growth and productivity by promoting chloroplast development.
A mini-review on how the COVID-19 pandemic affected intertemporal choice
Authors: Zhang Xinwen , Wu Ziyun , He Qinghua 
Published: October 2023

Abstract
The coronavirus disease (COVID-19) has extremely harmful effects on individual lifestyles, and at present, people must make financial or survival decisions under the profound changes frequently. Although it has been reported that COVID-19 changed decision-making patterns, the underlying mechanisms remained unclear. This mini-review focuses on the impact of the COVID-19 pandemic on intertemporal choice, and potential psychological, biological, and social factors that mediate this relationship. A search of the Web of Science electronic database yielded 23 studies. The results showed that under the COVID-19 pandemic, people tended to choose immediate and smaller rewards, and became less patient. In particular, people with negative emotions, in a worse condition of physical health, or who did not comply with their government restriction rules tended to become more "short-sighted" in behavioral terms. Future studies should examine more longitudinal and cross-cultural research to give a broad view about the decision-making change under the COVID-19 pandemic.
Environmental factors affecting the risk of generalization for ocular-onset myasthenia gravis: a nationwide cohort study
Authors: Wang Jingsi , Zhou Hongyu , Chen Hongxi , Feng Huiyu , Chang Ting , Sun Chao , Guo Rongjing , Ruan Zhe , Bi Fangfang , Li Jing , Wang Jianwen , Wang Kang , Ma Gaoting , Lei Shaoyuan , Wang Chunxiu , Wang Zhihong , Huang Feifei , Zhang Shu , Wen Qi , Wang Yaye , Sun Yanan , Li Yun , Xie Nairong , Liu Haoran , Jiang Yuting , Lei Lin , Fan Zhirong , Su Shengyao , Lu Yan , Di Li , Xu Min , Wang Min , Chen Hai , Wang Suobin , Wen Xinmei , Zhu Wenjia , Duo Jianying , Huang Yue , Zheng Deqiang , Da Yuwei 
Published: October 2023

Summary

Background
The environmental effects on the prognosis of ocular myasthenia gravis (OMG) remain largely unexplored.

Aim
To investigate the association between specific environmental factors and the generalization of OMG.

Design
The cohort study was conducted in China based on a nationwide multicenter database.

Methods
Adult patients with OMG at onset, who were followed up for at least 2 years until May 2022, were included. We collected data on demographic and clinical factors, as well as environmental factors, including latitude, socioeconomic status (per capita disposable income [PDI] at provincial level and education) and smoking. The study outcome was the time to the development of generalized myasthenia gravis (GMG). Cox models were employed to examine the association between environmental exposures and generalization. Restricted cubic spline was used to model the association of latitude with generalization risk.

Results
A total of 1396 participants were included. During a median follow-up of 5.15 (interquartile range [IQR] 3.37–9.03) years, 735 patients developed GMG within a median of 5.69 (IQR 1.10–15.66) years. Latitude of 20–50°N showed a U-shaped relation with generalization risk, with the lowest risk at around 30°N; both higher and lower latitudes were associated with the increased risk (P for non-linearity &lt;0.001). Living in areas with lower PDI had 1.28–2.11 times higher risk of generalization. No significant association was observed with education or smoking.

Conclusions
Latitude and provincial-level PDI were associated with the generalization of OMG in China. Further studies are warranted to validate our findings and investigate their potential applications in clinical practice and health policy.