bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Protein Glutaminase from Probiotic Escherichia coli Nissle 1917 for Enhancing the Functional Properties of Caseins
Authors: Zhang Zheng , Zheng Lihui , Li Yuxi , Jiao Shuchao , Wu Yelin , Jin Mingfei , Zhang Shuo , Gao Hongliang , Chang Zhongyi , Jiang Deming , Huang Jing 
Published: January 2024

Abstract

Protein glutaminase (PG; EC 3.5.1.44) is widely used in the food industry because it catalyzes the deamidation of peptide chain glutamine residues and enhances the functional properties of food proteins. Here, a strategy for PG production by probiotic Escherichia coli Nissle 1917 (EcN) is proposed. The yield of mature PG (mPG) was increased to 8.69 U/mL after testing a series of pSEVA vectors. The purified mPG showed significant deamidation activity against a wide range of protein substrates. Among these tested substrates, the functional properties of PG-modified casein were investigated. Deamidation of casein by PG was more effective at 60°C, pH = 7, and an enzyme-to-substrate ratio (E/S) of 5 U/g protein. Casein is deamidated up to 53.29%, which leads to a solubility of more than 90% for a 5% casein solution. Foam capacity can be nearly doubled. Emulsifiability, especially emulsification stability, is substantially improved. With increasing DD of casein, the α-helix and β-turn in the secondary structure of deamidated casein increased from 0–22.5%, and from 2.8–27.2% respectively, while β-fold and random decreased from 54.6–10.5%, and from 42.6–39.8% respectively. The enhancement of the absorbance values, endogenous fluorescence peaks, and surface hydrophobicity are due to the exposure of hydrophobic amino acids inside the tertiary structure of deamidated casein. Furthermore, deamidated casein particle size reduced while particle size homogeneity rose. After deamidation by PG, casein has achieved enhanced functional properties which improves its usability as a functional ingredient in the food industry.
Superficial circumflex iliac artery based fascial pedicle bi-lobed iliac bone flap transfer for reconstruction of foot composite tissue defects
Authors: Xu Lei , Ju Wen , Li Lei , Jin Qianheng , Zhang Yujun , Tang Linfeng , Fu Yi , Ruixing Ruixing , Ju Jihui 
Published: January 2024

Abstract
Background: To investigate the clinical efficacy of repairing the large-sized compound tissue defects in the foot with the free superficial circumflex iliac artery (SCIA) based fascial pedicle bi-lobed iliac bone flap.
Methods: A retrospective analysis from October 2009 to February 2017 was performed, and 11 patients were identified with large-sized compound tissue defects repaired with the free SCIA based fascial pedicle bi-lobed iliac bone flap. The size of wound ranged from 12 cm × 10 cm to 15 cm × 13 cm and the length of the bony defect was from 4 cm to 7 cm. The flap sizes ranged from 13 cm × 10 cm to 16 cm × 14 cm, and the length of the dissected iliac ranged from 5 cm to 8 cm. The donor sites were primarily closed.
Results: All 11 flaps survived engraftment including one case venous crisis occurred. After 6 months, only one of the cases resulted in swollen flaps which required repair. All other engrafted flaps were well appearing and the transplanted iliac healed smoothly, with a bone healing time of less than 12 weeks. The internal fixation was removed between 12 and 24 weeks post operation. Successful postoperative recovery of ankle joint flexion and extension resulted in normal gait.
Conclusions: The free SCIA-based fascial pedicle bi-lobed iliac bone flap repaired the large-sized compound tissue defect in the foot, resulting in repaired bone defect, wound coverage, low donor site morbidity, and recovery of function.
The research on the effect of dynamic tilt variation on the phase of cold atomic gravimeter
Authors: Chen Peijun , Zhou Yin , Zhu Dong , Wang Kainan , Zhang Can , Peng Shuping , Cheng Bing , Wu Bing , Lin Qiang 
Published: January 2024

Abstract

In recent years, absolute gravity comparison experiments between cold atomicgravimeter and traditional gravimeter have been carried out by combining coldatomic gravimeter with stable platform. Due to the working characteristics of thestable platform, the tilt angle of the cold atomic gravimeter will change slightlyin the process of dynamic measurement, and the measurement noise will be introduced. In order to improve the accuracy of dynamic measurement, this paper firstanalyzes the trajectory of the mirror and atom in the atomic gravimeter, obtainsthe phase expression of the atomic interferometer, evaluates the main terms ofthe gravity deviation by combining the data on the ship in the South China Sea,and then simplifies the higher-order small-terms in the main terms to deduce thesimplified form of the gravity deviation. Then, the 20-minutes data is divided into10 intervals, the power spectrum of the platform angle, angular velocity and carrier acceleration within each interval is solved. The root-mean-square value of thegravity deviation within each interval is solved by combining the transfer function of the window function. The variation trend of the root-mean-square valueis consistent with the trend of solving the gravity deviation through the trajectory. The conclusion provides the calculation method of gravity noise caused bydynamic tilt change, and also provides the basis for platform design.
Contrasting prognostic role of KEAP1-NFE2L2-CUL3 mutation in human Uterine Corpus Endometrial Carcinoma (UCEC)
Authors: Namani Akhileswar , Veeraiyan Durga Devi , Patra Tapas 
Published: January 2024

Abstract
Uterine corpus endometrial cancer (UCEC) is a third most common malignancy in women with a poor prognosis in advanced stages. In this study, we performed an integrated comparative analysis of exome and transcriptome data from The Cancer Genome Atlas (TCGA), Lung Adenocarcinoma (LUAD), and UCEC patients. Our multi-omics analysis shows that the UCEC patients carrying mutations in the KEAP1-NFE2L2-CUL3 genes were associated with better progression-free survival, whereas the KEAP1-NFE2L2-CUL3 mutation in LUAD showed poor outcomes. Functional annotations and correlative expression studies show that genes related to glutathione synthesis are differentially expressed in KEAP1-NFE2L2-CUL3 mutant UCEC compared to LUAD and may not produce enough glutathione to combat intracellular reactive oxygen species (ROS) and accumulate cysteine in UCEC. We hypothesize that the KEAP1-NFE2L2-CUL3 mutation in UCEC leads to uncontrollable ROS with di-sulfite stress, reflecting a favourable clinical outcome.
Research progress on the transportation of poly- and perfluoroalkyl substances (PFASs) in freshwater based on CiteSpace
Authors: Xiao Yulong , Shu Anping , Xie Tian , Dou Peng , Zhai Yujia , Bai Junhong , Cui Baoshan 
Published: January 2024

Abstract

Poly- and perfluoroalkyl substances (PFASs) have garnered extensive attention due to their dissolubility, stability, hydrophobicity, and oleophobicity, contributing to long-range transport of PFASs in the water. As an important part of the blue-green space system, freshwater plays a decisive role in ensuring environmental health. Hence, a comprehensive analysis of the current studies regarding the transportation of PFASs in freshwater is highly important for ecotoxicological assessment and pollution control. To explore research progress and hotspots of transportation of PFASs in freshwater, CiteSpace software was utilized in this literature to conduct publication growth, analysis of countries/regionss, analysis of publishing institutions, co-citation analysis, keywords bursting analysis, and keywords timeline analysis. The publication trends of the transportation of PFASs in freshwater were divided into the embryonic period (2005–2014) and the developing period (2015–2023). Institutions and authors from China were found to publish most literature, indicating China places a significant emphasis on the assessment of risks of PFASs in freshwater environments. Research hotspots shifted from transport behaviors and mechanisms to risk assessment and multimedia transportation. Understanding the transportation of PFASs in freshwater is crucial for assessing environmental impacts, ensuring water resource sustainability, and contributing to the development of management practices prioritizing sustainability, energy efficiency, and environmental responsibility.
Impact of source water quality on total organic carbon and trihalomethane removal efficiency in a water treatment plant: A case study of Upper Awash, Ethiopia
Authors: Assefa Emeru , Jabasingh Anuradha , Mulugeta Eyobel , Dessalegne Meseret , Teju Endale 
Published: January 2024

Abstract

This study addresses the limited understanding of factors affecting the efficiency of water treatment plants in reducing trihalomethane (THM) formation through total organic carbon (TOC) removal, highlighting significant challenges in improving treatment effectiveness. The aim of this study was to examine the influence of water quality on the efficiency of water treatment plants to remove TOC and reduce THM formation. Linear regression and correlation analyses were conducted to examine the relationship between water quality parameters and THM concentrations. The results showed that there was a negative relationship between turbidity, metals, and TOC concentration with TOC removal efficiency. Positive correlations were found between parameters and the formation of THMs in water. Of these parameters, water temperature was observed to have relatively less influence on THM formation. It was observed that seasonal variations in water quality affect the efficiency of TOC removal and THM content in treated water. THM levels in chlorinated water were found to be within the permissible range of the World Health Organization's drinking water quality guidelines. However, it is still important to maintain continuous monitoring and take measures to reduce THMs. The model demonstrated a strong correlation (R2 = 0.906) between predicted and measured THM values.
Assessment of groundwater quality and human health risk from nitrate contamination using a multivariate statistical analysis
Authors: Raheja Hemant , Goel Arun , Pal Mahesh 
Published: January 2024

Abstract

The present study explores the suitability of groundwater for drinking purpose and evaluates non-carcinogenic health risks for children, women, and men. For this purpose, 47 groundwater samples were collected and analyzed for physicochemical parameters, including nitrate concentration. The results revealed that nitrate concentration varied from 15 to 85 mg/L and that 48.93% of the groundwater samples exceeded the Bureau of Indian Standards’ limits of 45 mg/L. The spatial map of the pollution index of groundwater specifies that most of the study area lies in moderate to high pollution zones. Principal component analysis was also applied, and five principal components achieving eigenvalues more than 1 with a cumulative variance of 77.36% were found to be sufficient. The findings of non-carcinogenic risk rates range from 0.628 to 3.559 (average of 2.069) for children, 0.427 to 2.421 (average of 1.408) for women, and 0.362 to 2.049 (average of 1.191) for men, and approximately 80% of the population in the study region is exposed to high health risks. The health risk assessment specified that children in the study area are more susceptible than women and men. The findings of this study suggest that groundwater quality in the region has deteriorated, emphasizing the need for treatment before drinking.
Brain-derived Neurotrophic Factor Level and Gene Polymorphism as Risk Factors for Depression in Patients with type 2 Diabetes Mellitus- A Case-Controlled Study
Authors: Hammad Hany , Shaltout Inass , Fawzy Mai M. , Rashed Laila A. , Adel Noha , Abdelaziz Tarek S. 
Published: January 2024

Background:
Diabetes mellitus and depression are comorbidities that can be caused
by each other. Brain-derived neurotrophic factor (BDNF) functions as a neuronal growth factor.
It maintains the functional integrity of the nervous system.

Aim:
To study the possible association between BDNF levels and gene polymorphism with depression
in patients diagnosed with type 2 diabetes mellitus.

Methods:
The Elisa technique measured BDNF, and rs6265 gene polymorphism was detected
using real-time PCR. Depression was assessed utilizing a clinical interview tool designed to establish
the diagnosis of depression and differentiate it from other psychiatric diseases.

Results:
BDNF levels were significantly lower in patients with type 2 diabetes mellitus and
symptoms of depression than in patients with type 2 diabetes mellitus and no symptoms of depression
(82.6±16.1. Vs 122± 17.47, p< 0.001). There was a statistically significant difference
in BDNF levels in patients with diabetes among the three genotypes of the BDNF gene (p-value
< 0.001). Val/ Val carriers had the highest serum BDNF levels, and Met/ Met carriers had the
lowest serum BDNF levels. Subgroup analysis showed statistically significant genotype-related
differences in serum BDNF levels among the three subgroups in the Depression group. Val/ Val
carriers had the highest serum BDNF levels, and Met/ Met carriers had the lowest serum BDNF
levels. BDNF Val66Met polymorphism had no significant association with the presence of depression,
yet there was a trend towards significance (p = 0.05)

Conclusion:
In this pilot, Low levels of BDNF were associated with depression in patients with
type 2 diabetes. Carriers of the Met/ Met allele have the lowest serum BDNF levels. Multicenter
studies with more participants are required.
Understanding the Role of Galectin-1 in Heart Failure: A Comprehensive Narrative Review
Authors: Sotoudeheian Mohammad Javad , Mirahmadi Seyed-Mohamad-Sadegh , Pirhayati Mohammad , Azarbad Reza , Nematollahi Soroush , Taghizadeh Mehdi , Pazoki-Toroudi Hamidreza 
Published: January 2024

Abstract:Heart failure (HF) is the fastest-growing cardiovascular condition worldwide. The immune system may play a role in the development of HF since this condition is associated with elevated pro-inflammatory cytokine levels. HF is a life-threatening disease, and there is an increasing demand for diagnostic biomarkers, prognostic factors, and therapeutic agents that can help treat it. Galectin-1 (Gal-1) is the prototype galectin of the lectin family. Multiple signal transduction pathways are regulated by Ras proteins, which act as a molecular switch in cells. Gal-1 regulates T and B cell activation, differentiation, and survival. Gal-1 has been linked to inflammation. Activated T cells produce Gal-1 through an autocrine apoptotic mechanism involving MEK1/ERK and p38 MAPK. In the cardiovascular system, atherosclerosis is facilitated by Gal-1. Heart disease, myocardial infarction, hypertension, and stroke can be caused by atherosclerotic plaque. HF and heart hypertrophy are caused by decreased cardiac L-type Ca2+ channel activity. Deregulation of Gal-1 and CaV1.2 in pathological cardiac hypertrophy suggests a possible target for anti-hypertrophic therapy. Rat hypertrophic cardiomyocytes express Gal-1 and CaV1.2 channels simultaneously. It has been reported that diastolic dysfunction (DD) is associated with elevated Gal-1 levels. The high Gal-1 level in subjects led to the lowest cumulative survival as a composite endpoint. Incidences of HF, DD, and serum Gal-1 levels correlated significantly. The ejection fraction was negatively correlated with Gal-1 and CRP concentrations. Based on two different approaches in mice and humans, Gal-1 was identified as a potential mediator of HF.
Triple Action of Lignosulfonic Acid Sodium: Anti-protease, Antioxidant,
and Anti-inflammatory Effects of a Polymeric Heparin Mimetic
Authors: Al-Horani Rami A. , Aliter Kholoud F. 
Published: January 2024

Background:
Heparins are sulfated glycosaminoglycans that are used as anticoagulants
to treat thrombosis. Heparins exhibit other potential therapeutic effects, such as anti-inflammatory,
anti-viral, and anti-malarial effects. However, the strong anticoagulant activity of heparins poses a
risk of life-threatening bleeding, limiting their therapeutic use for other diseases beyond thrombosis.
To exploit the other effects of heparins and eliminate the bleeding risk, we explored an alternative
polymer called lignosulfonic acid sodium (LSAS), which acts as a sulfonated heparin mimetic.
LSAS targets factor XIa to exert an anticoagulant effect, and thus, unlike heparins, it is unlikely
to cause bleeding.

Methods:
This study investigated the multiple effects of LSAS to identify potential leads for complex
pathologies treatment. A series of chromogenic substrate hydrolysis assays were used to evaluate
the inhibition of three inflammation-related proteases by LSAS. Its chemical antioxidant activity
against the system of ABTS/hydrogen peroxide/metmyoglobin was also determined. Lastly, the
effect of LSAS on TNFα-induced activation of the NF-κB pathway in HEK-293 cells was also tested
to determine its cellular anti-inflammatory activity.

Results:
The results showed that LSAS effectively inhibited human neutrophil elastase, cathepsin
G, and plasmin, with IC50 values ranging from 0.73 to 212.5 μg/mL. Additionally, LSAS demonstrated
a significant chemical antioxidant effect, with an IC50 value of 44.1 μg/mL. Furthermore, at
a concentration of approximately 530 μg/mL, LSAS inhibited the TNFα-induced activation of the
NF-κB pathway in HEK-293 cells, indicating a substantial anti-inflammatory effect. An essential
advantage of LSAS is its high water solubility and virtual non-toxicity, making it a safe and readily
available polymer.

Conclusion:
Based on these findings, LSAS is put forward as a polymeric heparin mimetic with
multiple functions, serving as a potential platform for developing novel therapeutics to treat complex
pathologies.
PyComp: A Versatile Tool for Efficient Data Extraction, Conversion, and
Management in High-throughput Virtual Drug Screening
Authors: Sisakht Mohsen , Shahrestanaki Mohammad Keyvanloo , Fallahi Jafar , Razban Vahid 
Published: January 2024

Background:
Virtual screening (VS) is essential for analyzing potential drug candidates in drug discovery. Often, this involves the conversion of large volumes of compound data
into specific formats suitable for computational analysis. Managing and processing this wealth
of information, especially when dealing with vast numbers of compounds in various forms, such
as names, identifiers, or SMILES strings, can present significant logistical and technical challenges.

Methods:
To streamline this process, we developed PyComp, a software tool using Python's
PyQt5 library, and compiled it into an executable with Pyinstaller. PyComp provides a systematic way for users to retrieve and convert a list of compound names, IDs (even in a range), or
SMILES strings into the desired 3D format.

Results:
PyComp greatly enhances the efficiency of data extraction, conversion, and storage
processes involved in VS. It searches for similar compounds coupled with its ability to handle
misidentified compounds and offers users an easy-to-use, customizable tool for managing largescale compound data. By streamlining these operations, PyComp allows researchers to save significant time and effort, thus accelerating the pace of drug discovery research.

Conclusion:
PyComp effectively addresses some of the most pressing challenges in highthroughput VS: efficient management and conversion of large volumes of compound data. As a
user-friendly, customizable software tool, PyComp is pivotal in improving the efficiency and
success of large-scale drug screening efforts, paving the way for faster discovery of potential
therapeutic compounds.
Pharmacological Investigation of Anticonvulsant Effect of Artemisia Vulgaris on Pentylenetetrazole-Induced Epileptic Seizures in Zebrafish Experimental Model
Authors: Sharma Mahima , Kumar Godlaveti Vijay Narasimha , Gupta Pankaj 
Published: January 2024

Background:
Epilepsy is the most common neurological disorder, characterized by abnormal,
unpredictable, and recurrent seizures. In the alternative medicine system, Artemisia vulgaris has
been used to treat epileptic symptoms since its inception. However, scientific evidence concerning
its effect on the treatment of epileptic seizures is not available.

Objective:
The objective of the study is to evaluate the anti-convulsant potential of Artemisia vulgaris
in zebrafish experimental models.

Methods:
Zebrafish larvae and adult zebrafish were used as experimental models. Briefly, larvae and
adult zebrafish were exposed to 0.5% of the test medicine Artemisia vulgaris mother tincture (ϕ) and
its potencies (6 CH, 30 CH), and valproic acid for 1 hour. After 1 hour of exposure, they were exposed
to pentylenetetrazole to record different seizure scores from 1 to 5 using ANY maze video tracking
software.

Results:
Artemisia vulgaris 30CH in zebrafish larvae and Artemisia vulgaris 6 CH and 30 CH in adult
zebrafish delayed the latency score from score 1 to score 5 and were found effective against PTZinduced
locomotor activity and seizure duration and intensity.

Conclusion:
In conclusion, Artemisia vulgaris (6 CH and 30 CH) has the potential to be a novel
treatment for symptomatic epileptic seizures and could be a potential alternative drug candidate for
symptomatic treatment of epilepsy.
JHD205, A Novel Abemaciclib Derivative, Exerts Antitumor Effects on Breast Cancer
by CDK4/6
Authors: Ji Jing , Qin Jingting , Wang Xiaoshuo , Lv Mingxiao , Hou Xiao , Jing Aixin , Zhou Jiaojiao , Zuo Lingyi , Liu Wenwen , Feng Jing , Qian Qilan , Liu Yuanyuan , Wang Xiujun , Liu Bin 
Published: January 2024

Background:
Efficient targeted molecular therapeutics are needed for the treatment of triple-negative
breast cancer (TNBC), a highly invasive and difficult-to-treat form of breast cancer associated with a poor prognosis.

Objectives:
This study aims to evaluate the potential of selective CDK4/6 inhibitors as a therapeutic option for
TNBC by impairing the cell cycle G1 phase through the inhibition of retinoblastoma protein (Rb) phosphorylation.

Methods:
In this study, we synthesized a compound called JHD205, derived from the chemical structure of
Abemaciclib, and examined its inhibitory effects on the malignant characteristics of TNBC cells.

Results:
Our results demonstrated that JHD205 exhibited superior tumor growth inhibition compared to Abemaciclib
in breast cancer xenograft chicken embryo models. Western blot analysis revealed that JHD205 could dosedependently
degrade CDK4 and CDK6 while also causing abnormal changes in other proteins associated with
CDK4/6, such as p-Rb, Rb, and E2F1. Moreover, JHD205 induced apoptosis and DNA damage and inhibited
DNA repair by upregulating Caspase3 and p-H2AX protein levels.

Conclusion:
Collectively, our findings suggest that JHD205 holds promise as a potential treatment for breast
carcinoma.