bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenolic compounds in purple whole-wheat flour and bread: Comparative analysis
Authors: Valieva Alfia , Akulov Anton , Rumyantseva Natalya 
Published: December 2023

Whole-wheat flour of purple wheat varieties contains anthocyanins and other phenolic compounds with high antioxidant activity, which makes it a potential raw material for functional foods. The content and composition of phenolic compounds in whole-wheat flour depends on the genotype and weather conditions; in the bread, however, they also depend on the bread-making technology. This article offers a comparative analysis of phenolic compounds in purple whole-wheat flour and bread baked from this flour.
The study featured purple soft spring wheat (Triticum aestivum L.) of two varieties, Nadira and Line 193, which were harvested in 2016 and 2017, and corresponding bread samples. The antioxidant activity and phenolic content were measured spectrophotometrically while the qualitative analysis relied on the method of high-performance liquid chromatography (HPLC).
In the hot and dry year of 2016, the content of bound phenolic acids reached 2.0–2.4 mg/g dry weight in the flour of both genotypes. In the cool and humid year of 2017, the content of anthocyanins in the Nadira flour increased by 2.7 times and amounted to 0.65 mg/g dry weight. However, the corresponding bread sample had a much lower phenolic content: soluble phenolics were halved, anthocyanins dropped by 3–4 times, and bound phenolic acids went down by 7–17 times. The content of bound phenolic acids in the flour correlated positively with the content of free phenolic acids in the bread. The HPLC analysis revealed an increase in the content of free hydroxycinnamic acids in the bread: p-coumaric acid was the most abundant and amounted to 0.14–0.22 mg/g dry weight.
Conventional State Standard 27669-88 for bread making resulted in a total decrease in anthocyanins, bound phenolic acids, and most free phenolics. Therefore, this technology cannot be applied to functional bread. The results can help develop a breadmaking technology for purple wheat varieties.
Linking ecological characteristics with fish diversity, assemblage patterns and feeding guilds, and GIS applications along the temporal and spatial gradients in a large subtropical reservoir, India, for sustainable management
Authors: Alam Absar , Kumar Jeetendra , Sarkar Uttam Kumar , Jha Dharm Nath , Sahu Sanjeev Kumar , Sukla Das Shyamal Chandra , Srivastava Saket Kumar , Kumar Vijay , Das Basanta Kumar 
Published: December 2023

Abstract
The objective of the investigation was to explore the abundance, composition, and diversity patterns of the fish fauna along the temporal and spatial scale and study the influence of environmental parameters on the fish assemblage in the Rihand reservoir, a large sub-tropical Indian reservoir in India. On the temporal scale, the highest abundance was recorded in the summer season and the lowest in the monsoon season, while on the spatial scale, the highest abundance was recorded from the riverine and the minimum from the lacustrine zone. The highest species richness and diversity were recorded from the riverine zone followed by the transitional and lacustrine zones, respectively. The analysis of the variance of fish abundance revealed insignificant differences between the riverine and the transitional zones. The trophic guild analysis indicated the dominance of carnivores. The output of canonical correspondence analysis depicted the significant influence of the physico-chemical traits on the abundance of fish species. The transparency and specific conductivity were found to be the most critical factors affecting fish assemblages in the Rihand reservoir. This study generated important baseline ecological information that would be useful for the monitoring, conservation, and management of the reservoir ecosystem.
A variação ideológica dos partidos políticos brasileiros
Authors: Souza Ailton 
Published: December 2023

Associar a ideologia dos partidos aos espectros ideológicos de esquerda, centro ou direita pode ser considerado algo simples ou uma tarefa mais complexa quando considerarmos o amplo quadro de partidos e possibilidades analíticas de classificação. Inseridos neste contexto, este artigo discute estabilidade e volatilidade das classificações ideológicas, avaliando em que medida a ideologia dos partidos pode ser considerada estática ou volátil quando consideramos uma zona neutra? Em tal abordagem, faz-se o uso de um estudo qualitativo que analisa alguns dos principais estudos classificatórios. Destaca-se neste contexto a existência de definições relativamente “frágeis” ou meramente temporais quando analisadas em longo prazo. E da mobilidade bidirecional de legendas, embora estas não se movimentem até um polo totalmente inverso a sua posição inicial.
INVESTIGAÇÃO SOBRE OS MÉTODOS DE ASSEPSIA E EQUIPAMENTOS DE PROTEÇÃO INDIVIDUAL UTILIZADOS POR MANICURES E PEDICURES
Authors: Nascimento Lília Costa , Cavalcanti Adilma da Cunha , Mota Silva Miriam Maria , Silva Maria Carolina Barbosa Moura da , Cabral Hevelly Carlos , Santos Nathália Patrícia Almeida 
Published: December 2023

Introdução: As atividades laborais de manicures e pedicures geram riscos de exposições acidentais aos patógenos vinculados ao sangue como vírus, fungos e bactérias, devido à falta de adesão às medidas de biossegurança, e procedimentos de esterilização acarretando riscos à saúde dos profissionais e clientes. Objetivo: Verificar os conhecimentos das manicures e pedicures sobre os métodos de limpeza e esterilização de materiais, o uso de equipamentos de proteção individual e doenças relacionadas à profissão. Metodologia: Estudo transversal de abordagem quantitativa, realizado entre Outubro de 2017 a Janeiro de 2018, com 22 participantes finais. Incluídos os maiores de 18 anos, ambos os sexos, experiência mínima de um ano e residir em Nova Floresta. A técnica para seleção da amostra foi snowball. Resultados: A limitação de conhecimento sobre os riscos laborais na exposição de agentes infectantes, a baixa adesão no uso de equipamentos de proteção individual e a falta de padronização no processo de descontaminação, limpeza, desinfecção e esterilização dos instrumentais estão associados ao potencial risco de contaminação por agentes infecciosos, e ao acometimento de doenças infectocontagiosas por manicures, pedicures e clientela. Conclusões: Existem fragilidades no conhecimento destes profissionais, a maioria expõe métodos de antissepsia precários, devido a falta de sistematização e padronização dos procedimentos de limpeza, descontaminação dos instrumentais e o uso de equipamentos de proteção individual, além do conhecimento da profissão ter sido adquirido informalmente.
Palavras-Chave: Contenção de Riscos Biológicos; Equipamento de Proteção Individual; Esterilização; Centros de Embelezamento e Estética; Segurança do Paciente.
EFEITO DO pH DO GEL CLAREADOR DE CONSULTÓRIO NA COR E NA SENSIBILIDADE DENTÁRIA
Authors: Araújo Isabela Dantas Torres de , Queiroz Dilana Mota de , Assunção Isauremi Vieira de 
Published: December 2023

Introdução: Sabe-se que a busca pela estética é algo cada vez mais crescente. Dentre os procedimentos mais procurados na odontologia estética, destaca-se o clareamento dental de consultório. Diante disso, temos agentes clareadores de diferentes pHs e a carência de estudos sobre seus efeitos na efetividade clareadora e na sensibilidade. Objetivo: Avaliar a sensibilidade dentária e a eficácia clareadora de géis clareadores à base de peróxido de hidrogênio a 35% com diferentes pHs. Metodologia: Trata-se de um relato de três casos, descritivo e observacional, do tipo boca dividida (split-mouth) para cada estratégia clareadora (gel clareador com pH básico e gel clareador com pH ácido). Foram avaliados três pacientes de 25, 26 e 27 anos de idade. Realizou-se registro de cor por meio da escala VITA Classical e avaliação da sensibilidade dentária pela escala visual analógica. Resultados: Todos os pacientes avaliados apresentaram cor A3 no registro de cor inicial e, após o clareamento dental, atingiram a cor A1. Todos relataram uma maior sensibilidade no hemiarco direito (local onde foi aplicada o gel clareador Whiteness HP que possui pH ácido. Dois pacientes relataram sensibilidade dentária no hemiarco esquerdo em que foi aplicado o gel clareador de pH básico. Conclusões: Observa-se a eficácia clínica dos géis clareadores de consultório à base de peróxido de hidrogênio a 35% na estabilidade de cor após o tratamento clareador, independente do seu pH. Ademais, nota-se que o gel clareador de pH básico promoveu menor sensibilidade pós-operatória.
DIABETES TIPO II. A TERAPIA PERIODONTAL NÃO CIRÚRGICA PODE FAVORECER O CONTROLE GLICÊMICO DO PACIENTE?
Authors: Teixeira Alessandra , Maciel Euler 
Published: December 2023

Introdução: A doença periodontal corresponde à condição que acomete os tecidos de proteção e/ou suporte do dente através de uma inflamação crônica causada por patógenos. Esta condição pode ser modificada ou associada às doenças sistêmicas, como por exemplo, o diabetes mellitus tipo II (DM2). Objetivo: Avaliar quais os efeitos da terapia periodontal não cirúrgica sobre o controle glicêmico de pacientes diagnosticados com DM2. Metodologia: Revisão integrativa elaborada a partir de pesquisas clínicas randomizadas indexadas nas bases de dados Pubmed, Embase, Cochrane, Web of Science e BVS, na qual foram utilizados os descritores “periodontal diseases treatment”, “glycemic control” e “metabolic control”. Resultados: Dos trabalhos avaliados, seis foram selecionados para compor a revisão, tendo em vista os critérios de inclusão e exclusão estabelecidos. Logo, é notório que a terapia periodontal básica indica melhora no controle glicêmico dos pacientes com DM2, de acordo com análise da HbA1c e PCR, portanto, com base nos resultados dessa pesquisa, o tratamento periodontal não cirúrgico parece contribuir para o controle metabólico. Apesar disso, alguns estudos se opõem ao resultado do controle glicêmico desses pacientes, reforçando a existência de variáveis que interferem nos resultados da pesquisa, como os níveis de hemoglobina, estágio da doença periodontal, amostra, dieta e atividade física dos pacientes. Conclusões: O resultado deverá ser avaliado com maior cautela, tendo em vista as possibilidades de variáveis presentes nesse tipo de pesquisa. Por fim, ensaios controlados devem ser realizados para alcançar um maior esclarecimento a respeito dos efeitos da terapia periodontal não cirúrgica no controle glicêmico de pacientes com DM2.
Palavras-Chave: Doenças periodontais; índice glicêmico; hemoglobina A glicada.
TELEMONITORAMENTO DOS IDOSOS DE NATAL-RN NA ATENÇÃO PRIMÁRIA DURANTE A PANDEMIA DA COVID-19: ESTUDO LONGITUDINAL DESCRITIVO
Authors: Amancio Amanda de Medeiros , Sousa Lucas Cavalcante de , Rodrigues Fillipe  Morais , Medeiros Ruan Garcia de , Guerra Eliana Costa , Ferreira Maria Ângela Fernandes 
Published: December 2023

Introdução: A telessaúde se popularizou como uma importante ferramenta na avaliação remota e de prestação de cuidados em saúde. Uma de suas vertentes consiste no monitoramento remoto, também chamado de telemonitoramento ou televigilância, que se difundiu, sobretudo para grupos populacionais vulneráveis, como a população idosa, em especial durante a pandemia da COVID-19. Objetivo: Conhecer o perfil sociodemográfico, o estado de saúde e os comportamentos relacionados à saúde dos idosos monitorados durante a pandemia da COVID-19 no município de Natal/RN. Metodologia: Estudo longitudinal descritivo, com três momentos de observação. A amostra foi composta por idosos adscritos a 22 unidades de saúde de Natal-RN, acompanhados por meio de ligações telefônicas, de agosto de 2020 a julho de 2021. A análise dos dados foi realizada através do software Epi Info™, versão 7.2.4, a partir de frequências absolutas e relativas, medidas de tendência central e dispersão (média e desvio padrão). Resultados: Participaram do estudo 1.348 idosos. A maioria era do sexo feminino (63,7%), com média de 70 anos, morava acompanhada (81,8%), esteve assintomática (77,0%), possuía comorbidades (81,5%) e dependia de medicações de uso contínuo (81,9%). Os sintomáticos diminuíram ao longo dos três momentos avaliados e menos de 1% evoluiu ao óbito. Conclusões: O telemonitoramento contribuiu com a longitudinalidade do cuidado, proporcionando a busca ativa contínua de idosos sintomáticos e fortalecendo as atividades das Unidades de Saúde.
Palavras-Chave: Telemonitoramento, Idoso, Comportamentos Relacionados com a Saúde, Atenção Primária à Saúde, COVID-19.
Synthesis of Polyalkoxymethylenedioxy-1,4-naphthoquinones from Parsley Seed
Metabolite
Authors: Varakutin Alexander E. , Muravsky Egor A. , Khrustalev Victor N. , Samet Alexander V. , Semenov Victor V. 
Published: December 2023

Abstract:
A convenient synthesis of polyalkoxy-1,4-naphthoquinones, good starting materials
for analogues of natural quinones, has been developed. Bromonaphthoquinone, more
stable than its hydroxylated analogue, turned out to be a convenient and accessible key
synthon for the preparation of various polyalkoxylated naphthoquinones. The obtained quinones
could be useful starting materials for the preparation of biologically active polymethoxy-
and hydroxy-methylenedioxynaphthoquinones.
ClpP Peptidase as a Plausible Target for the Discovery of Novel
Antibiotics
Authors: Bhardwaj Smriti , Roy Kuldeep K. 
Published: December 2023

Abstract:
Antimicrobial resistance (AMR) to currently available antibiotics/drugs is a global
threat. It is desirable to develop new drugs that work through a novel target(s) to avoid drug resistance.
This review discusses the potential of the caseinolytic protease P (ClpP) peptidase complex
as a novel target for finding novel antibiotics, emphasising the ClpP’s structure and function. ClpP
contributes to the survival of bacteria via its ability to destroy misfolded or aggregated proteins. In
consequence, its inhibition may lead to microbial death. Drugs inhibiting ClpP activity are currently
being tested, but no drug against this target has been approved yet. It was demonstrated that Nblocked
dipeptides are essential for activating ClpP’s proteolytic activity. Hence, compounds mimicking
these dipeptides could act as inhibitors of the formation of an active ClpP complex. Drugs,
including Bortezomib, Cisplatin, Cefmetazole, and Ixazomib, inhibit ClpP activation. However,
they were not approved as drugs against the target because of their high toxicity, likely due to the
presence of strong electrophiles in their warheads. The modifications of these warheads could be a
good strategy to reduce the toxicity of these molecules. For instance, a boronate warhead was replaced
by a chloromethyl ketone, and this new molecule was shown to exhibit selectivity for
prokaryotic ClpP. A better understanding of the structure and function of the ClpP complex would
benefit the search for compounds mimicking N-blocked dipeptides that would inhibit ClpP complex
activity and cause bacterial death.
Discordance of Aqueous/Plasma HIV Replication on ART
Authors: Che Xin , Wang Luoziyi , Ma Weimei , Wang Rui , Wang Zhiliang 
Published: December 2023

Background:
The study was conducted to analyze HIV dynamics across blood–retinal
barrier (BRB) and the relevant risk factors for HIV-associated ocular complications.

Methods:
This study included a case series of 40 HIV-positive patients with ocular lesions, which
were studied retrospectively. Clinical and laboratory examinations included plasma and intraocular viral load (VL).

Results:
HIV VL on paired aqueous/plasma samples was available for 40 patients. Aqueous VL
was negatively associated with antiretroviral treatment (ART) duration (p = 0.02 and p < 0.05),
and plasma VL was independent of ART duration (p = 0.53). An aqueous/plasma discordance was
found in 19/40 (47.5%) patients, eight of whom (20%) had detectable aqueous VL despite a suppressed plasma VL (escape). There were significant differences in CD4+
T-lymphocyte levels
(p=0.011 and p < 0.05) and ART duration (p=0.007 and p < 0.05) between the patients with HIVassociated ocular complications and the patients without.

Conclusion:
This study provides a rationale for initiating ART early in the course of infection to
reduce HIV VL in the aqueous humor, and raises the possibility of the ocular sanctuary where
HIV replicates. Meanwhile, early and standard ART would be an optimal option to protect against
ocular opportunistic infection.
Biological Activities of Zinc Oxide Nanoparticles Green Synthesized Using
the Aqueous Extract of Dracocephalum kotschyi Boiss
Authors: Mirzania Foroogh , Salimikia Iraj , Ghasemian Yadegari Javad , Marzban Abdolrazagh , Firouzi Amirmasoud , Nazarzadeh Alireza , Aalaei Javid 
Published: December 2023

Background:
Dracocephalum kotschyi Boiss. is known as a native medicinal plant of Iran.

Objective:
In this study, aqueous extract of D. kotschyi was used to synthesize ZnO-NPs. To produce
ZnO-NPs, aerial parts of D. kotschyi were powdered and then macerated for obtaining aqueous extract, after that, aqueous extract was used to reduse zinc nitrate to ZnO-NPs.

Methods:
To confirm nanoparticles synthesis, SEM, TEM, UV-Vis, FTIR, and XRD were used. The
synthesized ZnO-NPs were studied for antimicrobial activities by microdilution method for calculating MIC and MBC. Analysis of ZnO-NPs confirmed successful synthesis by extract of D. kotschyi.

Results:
The sizes of ZnO-NPs were estimated 50-200 nm in diameter. Antibacterial and antifungal
experiments showed potent activities against Staphylococos aureus, Pseudomonas aeruginosa and
Candida albicans. The results of the studies showed that the nanoparticles synthesized with the aqueous extract of D. kotschyi have a much greater antimicrobial effect than the aqueous extract of D.
kotschyi and zinc nanoparticles, each alone (MIC values 3.7 to 7.5 mg/ml).

Conclusion:
The noteworthy point is that the inhibitory rate of synthesized zinc oxide nanoparticles
is higher compared to broad-spectrum antibiotics, such as chloramphenicol (MIC values 15 mg/ml).
Determining the therapeutic and toxic dose of this product for humans requires further investigation
and clinical trials.
Study of the Immunogenicity of Combination of Recombinant RBD (Omicron) and Nucleocapsid Proteins of SARS-CoV-2 Expressed in Pichia Pastoris
Authors: Azimova Shakhnoz , Sasmakov Sobirdjan , Eshboev Farkhod , Abdurakhmanov Jaloliddin , Ashirov Oybek , Khasanov Shukhrat , Makhnyov Artyom , Baymirzaev Asadali , Alimukhamedova Orzigul , Sadullaev Tulkin , Yusupova Elvira , Nasriddinov Khusan , Terenteva Ekaterina , Umarova Mukaddas , Khamidova Umida , Piyakina Galina , Lysova Elena , Ikramov Saidazim , Tosheva Nigora , Dolimov Khayotjon , Gaynazarova Saodat , Yarilkaganova Aygul 
Published: December 2023

Background:
SARS-CoV-2 is a virus responsible for the COVID-19 pandemic that began in late 2019. This pandemic has had a devastating impact worldwide, resulting in over 6.95 million deaths. The development of effective vaccines against the virus is crucial for preventing infection and reducing the severity of the disease. Objective: This study aimed to obtain the recombinant receptor-binding domain (RBD) and the nucleocapsid (N) proteins of SARS-CoV-2 as well as assess the immunogenicity of the combination of these recombinant proteins.

Methods:
The recombinant plasmids encoding the receptor-binding domain (RBD) of the spike protein of the Omicron variant and the nucleocapsid protein of SARS-CoV-2 were cloned into the yeast Pichia pastoris. The optimal fermentation conditions were established for recombinant P. pastoris strains. The methods for the isolation and purification of the target recombinant RBD and nucleocapsid proteins were developed. The immunogenicity of the purified recombinant proteins was evaluated by injecting them into mice and analyzing the specific IgG antibody responses using ELISA.

Results:
The study found that RBD and N proteins, as well as their combination, showed antigenic specificity and were highly immunogenic in mice. The immunogenicity was measured by determining the antibody titer, which represents the concentration of antibodies produced in response to the antigen. The antibody titers were 1:60000 for both RBD and N proteins, and 1:80000 for their combination.

Conclusion:
These findings suggest that the expressed proteins could be potential candidates for the development of vaccines or immunological diagnostic test kits for combatting or detecting the Omicron variant of SARS-CoV-2.
Sorbus Aucuparia L. Fruit Extract and its Cosmetics – As Promising Agents for Prophylactic and Treatment of Pyodermitis: Phytochemical and Microbiological Research
Authors: Maliuvanchuk Svitlana , Grytsyk Andriy , Melnyk Mariia , Kutsyk Roman , Yurkiv Khrystyna , Raal Ain , Koshovyi Oleh 
Published: December 2023

Background:
The problem of acne is relevant. The market mostly represents synthesized substances for prevention and treatment, but medical cosmetics based on plant-origin raw material, especially fruits, can be offered. The fruits of Sorbus aucuparia L. are rich in biologically active substances, which turned out to be unique in solving problems of acne.

Objective:
The aim of the research was to study the phenolic composition and microbiological activity of the S. aucuparia fruit extract and the proposed medicinal cosmetics to prove the possibility of their use for the treatment of pyodermitis.

Methods:
The phenolic compounds of the S. aucuparia fruit extract obtained with 70% ethanol solution were studied by HPLC. The microbiological research was carried out by methods of diffusion into agar.

Results:
In the S. aucuparia extract gallic acid, gallocatechin, epigallocatechin, catechin, epicatechin, epicatechin gallate, ellagic acid, hyperoside, rutin, quercetin-3-D-glucoside, chlorogenic, caffeic, ferulic and р-coumaric acids, umbelliferone were identified and quantified. The antimicrobial activity of the S. aucuparia extract has been established. The synergism of antimicrobial activity of 1/4 and 1/64 minimal suppression concentration erythromycin against all tested Staphylococci strains was manifested by the extract. Biological active substances of S. aucuparia fruits more effectively restore sensitivity to erythromycin in Staphylococci with low macrolide resistance of Staphylococci (MLS-resistance) due to blocking reflex mechanisms.

Conclusion:
The S. aucuparia fruit extracts are rich in phenolic compounds and show antimicrobial activity against all used test-strains of microorganisms. The results of the study indicate their high antimicrobial activity against the main pathogens of pyodermitis - S. aureus, S. epidermidis, and Propionibacterium acnes.