bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of Targeted Oxygen Therapy for Non-Tension Pneumothorax in Full-Term Neonates
Authors: Ghuzlan Alaa , Ali Wajih , Jouni Oday 
Published: October 2023

Abstract
Background: Pneumothorax is deemed a medical exigency that bears substantial morbidity and mortality. Recently, efficacious management approaches have been reported for pneumothorax in hemodynamically stable neonates, involving the utilization of targeted oxygen to circumvent drainage and its associated complications.
Objective: The purpose of this investigation was to assess the outcomes of oxygen therapy as a conservative approach to treating pneumothorax.
Materials and Methods: A prospective interventional study was conducted over one year (2021-2022) at Tishreen University Hospital in Lattakia-Syria. The study enrolled full-term neonates diagnosed with non-tension unilateral pneumothorax, who were treated by administering oxygen via nasal cannula while closely monitoring for any indications of deterioration in the condition. The primary outcome measured was the time to resolution of pneumothorax through chest X-ray (CXR). The study was approved by Scientific Research Ethics Committee at Tishreen University on 1/6/2021. Written informed consent was obtained from the patients legal guardians.
Results: Among the 23 neonates diagnosed with pneumothorax, 13 (56.5%) were male and 10 (43.5%) were female, exhibiting an average age and weight of 31.95±28.8 hours and 2803.04±336.01 g, respectively. Clinical signs manifested as tachypnea in all cases (100%), followed by intercostal retraction in 21 cases (91.3%) and subcostal retraction in 9 cases (39.1%). Pneumothorax was located on the right side for 11 cases and on the left side for 12 cases, with a mean volume of 10±4.4%. Consequently, there was a significant reduction in inspired oxygen concentration during follow-up periods(p:0.0001), resulting in an average resolution rate of approximately 17.70±8.1%/12 hour. observed across patients' data sets; complete resolution occurred within12 hours for11 cases(47 .8 %)and within 24 hours for the other cases.
Conclusion: In conclusion, the present investigation has unearthed that resorting to oxygen therapy serves as a means of expediting the resolution of pneumothorax among neonates who are in stable condition.
Establishment and validation of rat model of cervical spondylosis with unbalanced dynamic and static forces
Authors: Qian Jiaming , Wang Xiaole , Fang Ting , Ren Changan , Qiu Zikai , Zhao Xiaolan , Yang Yuqian , Zhu Jinchao , Deng Biao , Liu Fushui 
Published: October 2023

Abstract
Background: Cervical spondylosis (CS) presents a trend of generalization and rejuvenation, which has a major impact on society and economy. This study aimed to construct a rat model of CS and verify its reliability.
Methods: Twelve 6-month-old SD rats were selected and divided into normal group and model group according to the random number table method. The rats in the model group were modeled with the method of unbalanced dynamic and static forces. After three months, X-ray films and open-field experiments were performed. Rats were sacrificed and splenius head muscles were taken for HE staining and qPCR.
Results: Compared with the normal group, cervical degeneration score was significantly increased in the model group （P < 0.05）; HE staining suggested that the muscle fiber hyperplasia was accompanied by inflammatory cell infiltration. The open-field experiments showed that the total distance and average speed of the model group were smaller (P < 0.05, P < 0.05); the total rest time was longer （P < 0.05）. In the expression of FGF7, FGF9 and KDR mRNA in the splenius head muscles of rats, the model group was less (P < 0.001, P < 0.01, P < 0.01).
Conclusion: The rat model of CS with unbalanced dynamic and static forces was successfully established. The pathogenesis of cervical spondylosis has been also preliminarily studied.
Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning
Authors: Zhang Li , Yan Qing , Lin Miao , He Juanjuan , Tian Jie , Chen Zhihan , Hong Fuyuan 
Published: October 2023

Abstract
Background: Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE) with poor treatment outcomes. The role and underlying mechanisms of ferroptosis in LN remain largely unknown. We aimed to explore ferroptosis-related molecular subtypes and assess their prognostic value in LN patients.
Methods: Molecular subtypes were classified on the basis of differentially expressed ferroptosis-related genes (FRGs) via the Consensus ClusterPlus package. The enriched functions and pathways, immune infiltrating levels, immune scores, and immune checkpoints were compared between the subgroups. A scoring algorithm based on the subtype-specific feature genes identified by artificial neural network machine learning, referred to as the NeuraLN, was established, and its immunological features, clinical value, and predictive value were evaluated in patients with LN. Finally, immunohistochemical analysis was performed to validate the expression and role of feature genes in glomerular tissues from LN patients and controls.
Results: A total of 10 differentially expressed FRGs were identified, most of which showed significant correlation. Based on the 10 FRGs, LN patients were classified into two ferroptosis subtypes, which exhibited significant differences in immune cell abundances, immune scores, and immune checkpoint expression. A NeuraLN-related protective model was established based on nine subtype-specific genes, and it exhibited a robustly predictive value in LN. The nomogram and calibration curves demonstrated the clinical benefits of the protective model. The high-NeuraLN group was closely associated with immune activation. Clinical specimens demonstrated the alterations of ALB, BHMT, GAMT, GSTA1, and HAO2 were in accordance with bioinformatics analysis results, GSTA1 and BHMT were negatively correlated with the severity of LN.
Conclusion: The classification of ferroptosis subtypes and establishment of protective model may a foundation for the personalized treatment of LN patients.
Determination of Oxidative Stress Responses in Dreissena polymorpha by the Effect of Temperature in Combined Rare Earth Elements (Terbium, Lanthanum, Gadolinium and Praseodymium)
Authors: SERDAR Osman , AYDIN Ayse Nur 
Published: October 2023

Abstract
The continuous development of industry and industry drives people to seek new resources, and for this purpose, the usage areas of Rare Earth Elements (REE), whose usage areas are increasing day by day, are increasing rapidly. Increasing concentrations of REEs as a result of increased use create pollution in the environment and harm living organisms. This pollution interacts with increasing temperature and causes more negative synergistic effects of the pollutant in the environment and the living body. In this study, sublethal concentration values were determined by literature review and the concentration value was determined as 125 mg/L. It is aimed to investigate some oxidative stress and antioxidant responses of Terbium, Lanthanum, Gadolinium and Praseodymium REEs in Dreissena polymorpha at 125 mg/L concentration at 3 different temperatures (16, 18, 20 0C) with biomarkers. For this purpose, a 24-hour and 96-hour trial design was created and 7 D. polymorpha were used in each trial group, and the application experiments were carried out in 3 repetitions. The samples, whose trial phase was finished, were kept at -80 degrees until analysis. In this study, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzyme activities and gulutathion (GSH) and Thiobarbituric acid (TBARS) level biomarker responses were determined with ELISA test microplate reader. CAYMAN brand SOD (Catalog No 706002), CAT Catalog No 707002) and GPx (Catalog No 703102), GSH (Catalog No 703002) and TBARS (Catalog No 10009055) were used in the study. According to the study data, statistically significant decreases were observed in SOD and CAT activities in the oxidative stress responses of REEs on D. polymorpha with increasing temperature, while there was no significant change in GPx activities. It was determined that there were increases in TBARS levels and decreases in GSH levels.
Obtaining and study of biodegradable granules loaded with oxamyl as eco-friendly preparation for Globodera rostochiensis control
Authors: Sukhanova A.A. , Boyandin A.N. , Ertiletskaya Natalya Leonidovna , Churakov A.A. 
Published: October 2023

Abstract

Potato cyst nematode (PCN) poses a great challenge for potato cultivation throughout the world. Being a rather difficult parasite due to a number of its physiological features, specifically a long-term viability of the eggs, PCN causes drastic damage to worldwide agriculture. Development of long-acting preparations deposited with nematicidal ingredients could be a novel prospective approach for PCN management. In this work, composite granules based on polycaprolactone and montmorillonite (PCL/MMT) (100:0; 90:10; 75:25; 50:50) were studied as carriers for oxamyl nematicide (~ 10 wt.% of granules). It was shown that the obtained granules are able to maintain the activity of oxamyl for 20 days and facilitate its gradual release. The maximum release of oxamyl was noted for PCL/MMT 50% granules and amounted to 82.6 µg/ml. The nematicidal effect of PCL/MMT 50% granules with oxamyl against G. rostochiensis has been demonstrated on potato variety "Krasnoyarskiy ranniy".
Development of scaffold-free tissue-engineered constructs with serum-free media from mesenchymal stem cell-derived cells for cartilage repair and long-term preservation
Authors: Maeda Satoshi , matsumoto masaya , Segawa Kotaro , Konishi Hiromu , Iwamoto Kaori , Nakamura Norimasa 
Published: October 2023

Abstract

Background: Synovial mesenchymal stem cells (sMSCs) have great therapeutic potential for cartilage repair and have been used for clinical application. However, therapeutic design considering products effectively without loss efficacy and safety with eliminated unknown factors or adverse effects remains a challenge. In addition, because long-term preservation is indispensable to maintain high levels of cell-based products until implantation, factors that affect quality must be evaluated and demanded to reduce their fluctuations throughout the distribution. This study aimed to investigate the properties and feasibility of novel scaffold-free tissue-engineered constructs using serum-free media and develop long-term preservation methods considering logistic and distribution strategies.
Methods: sMSCs obtained from the human synovium were cultured in serum-free media, seeded at high density in a monolayer, and finally developed as a sheet-like construct called “gMSC1.” The properties of the frozen gMSC1 (Fro-gMSC1) using a control rate freezer were compared with those of refrigerated gMSC1 (Ref-gMSC1). gMSC1s were examined by cell number, viability, and surface antigen expression profile by flow cytometry, immunostaining, and scanning electron microscopy (SEM) observation. Chondrogenic differentiation potential was analyzed by quantitative real-time polymerase chain reaction and quantification of glycosaminoglycan content in the pellet culture. Xenografts into the cartilage defects in rat knees were evaluated by histological staining.
Results: gMSC1 showed nearly similar properties independent of the preservation conditions. The animal experiment demonstrated that the defect could be filled with cartilage-like tissue under both gMSC1 application groups at approximately the same level with good integration to the adjacent tissue, suggesting that gMSC1 was formed and replaced the cartilage. Furthermore, several chondrogenesis-related factors were significantly secreted inside and outside the gMSC1. The morphological analysis of Fre-gMSC1 revealed comparable levels of quality against fresh gMSC1 in immunostaining and SEM observations. Thus, if cryopreserved, gMSC1, with no complicated materials or processes, could have sustained cartilage repair capacity under stable quality attributes and no adverse effects on efficacy or safety.
Conclusions: gMSC1 is a prominent candidate in novel clinical practice for cartilage repair, allowing for large quantities to be manufactured at one time and preserved for a long term by freezing.
Survival niches of Pseudomonas savastanoi pv. glycinea, causal agent of bacterial blight in soybean (Glycine max L.)
Authors: de Melo Luana Laurindo , Nascimento Daniele Maria do , Soman José Marcelo , Silva João César Lourencetti da , Sartori Maria Márcia Pereira , Maringoni Antonio Carlos , Júnior Tadeu Antônio Fernandes da Silva 
Published: October 2023

Abstract

Bacterial blight caused by Pseudomonas savastanoi pv. glycinea (Psg) is a prevalent disease in soybean crops worldwide, and crop rotation is a potential management strategy. However, the importance of alternative crops and soil as hosts of Psg is not well understood. This study aimed to assess the survival of Psg in four soil types, the phyllosphere and rhizosphere of 12 crops rotated with soybean, and endophytic colonization of crops by Psg. The pathogenic strain Soj. 1462 of Psg, resistant to rifampicin, was used in all experiments, and PCR confirmed bacterial survival. Psg survived for a maximum of two days in all soil types. In the phyllosphere, soybean had the longest Psg survival (70 days), followed by white oat, black oat, ryegrass, sunflower, and sorghum (35 days). In the rhizosphere, the longest Psg survival was obtained for white oat, black oat, ryegrass, barley, corn, soybean, and wheat (28 days), and less than 21 days for all other crops. In endophytic colonization, Psg survived for 70 days in soybean, white oat, black oat, ryegrass, corn, and sorghum, and between 42 to 56 days in other crops, when inoculated in artificially wounded plants. In plants inoculated by spray, the highest Psg survival was in ryegrass and soybean (70 days), and between 14 to 63 days in other crops. Cultivating sunn hemp, common bean, and radish in succession with soybean is recommended to reduce Psg inoculum since the survival periods recorded for these crops were lower.
Variable Clinical Presentation of DCLRE1C Deficiency from Childhood to Adulthood
Authors: Hazar Esra , Karaselek Mehmet Ali , Kapakli Hasan , Dogar Oznur , Artac Hasibe , Fındık Sıdıka , Arslan Sevket , Uygun Vedat , Guner Sukru , Reisli Ismail , Keles Sevgi 
Published: October 2023

Abstract
In this study, we aimed to report long term follow up of our patients with DCLRE1C hypomorphic mutation including children and adults with leaky SCID. Eighteen patients, aged 6–29 (11 children, 7 adults), were enrolled in the study. Clinical and immunological features, including immunoglobulin levels, T and B cells, natural killer cell subsets, Treg cell ratios/markers, and cytokines, were assessed pre- and post-HSCT and compared with healthy controls. Recurrent infections (78%) and skin findings including granulomatous skin lesions, warts, vitiligo (61%) were the most frequently observed clinical findings. Autoimmune diseases were observed in 33% and malignancy in 17% of the patients. Most patients had low serum IgA and B and T cell lymphopenia at the first admission. RTE, Tnaive, Bnaive, CD56dimCD16+ cell ratios were significantly lower in the patients compared to control, however, TFH and Th1 (IFN-γ) cell ratios were significantly higher than the control. Although, Treg ratio and its functional receptors tend to be high but not significant. Eleven patients (61.1%) were treated with HSCT. Mean follow-up times of transplant patients was 46.41± 25.77 months. Patients with hypomorphic DCLRE1C mutations can present with variable clinical and laboratory findings at different ages. Our study showed a Th1 dominant immune response in patients before and after HSCT. Increased IFN-γ and TFH cells ratio could be a reason for chronic inflamation and autoimmunity developing before and after HSCT. Long term follows up of those patients after HSCT will help to better understanding of the disease and its pathophysiology.
Dynamic changes of regional brain activity in adolescents with internet gaming disorder
Authors: Lang Yan , Wang Rui-qi , Li Yan-ge , Zhang Yi-bo , Wu Jie , Zhao Tao 
Published: October 2023

Abstract

This study aimed to explore the dynamic changes of regional brain activity in adolescent with Internet Gaming Disorder (IGD) based on the analysis of dynamic low-frequency amplitude. Methods: Forty-four patients with IGD and 50 age-, sex-, and education-matched healthy controls (HC group) were recruited for resting state magnetic resonance imaging analysis of their regional brain activities. The collected imaging data was analyzed using two independent-sample t-test in which the MATLAB software was used to extract dynamic amplitude of low-frequency fluctuation (dALFF) values of abnormal active brain regions. Person correlation analysis were used to analyze the Internet Addiction Test (IAT) and the SCL-90 scores. Finally, IAT scores, factor scores in the SCL-90 scale and dALFF values of abnormal brain regions in the IGD group were analyzed for moderating effects using the process 4.1 plug-in of the SPSS26 statistical software. p < 0.05 suggests the existence of regulatory effects. Results: IGD group exhibited alterations in the local dynamic activity of signals in several brain regions, compared to HC group. Reduced dALFF values were mainly found in the inferior temporal gyrus, precuneus, posterior cerebellum lobe, and cerebellum vermis regions. The results of dynamic low-frequency amplitude analysis showed a negative correlation between left precuneus dALFF values and IAT scores in the IGD group (r=-0.403, p = 0.008). The results of the correlation analysis with the clinical scale indicated that the dALFF values in the left posterior cerebellum lobe (cerebellum region VI) were negatively correlated with the interpersonal sensitivity factor score (r=-0.314, p = 0.034) and depression factor score (r=-0.342, p = 0.033) in the SCL-90 scale. Conclusions: IGD in adolescents may have an impact on their mental health, and the severity of Internet gaming disorder may play a moderating role between the posterior cerebellum lobe and the mental health status of adolescent Internet addicts, which may provide some useful information in the future to explain clinical phenomena such as Internet addicts with different degrees of emotional problems.
Stocking Density of the Pangasius Hypophthalmus Reared in Raceway System
Authors: Rodrigues Juliano Silva , Gomides Pedro Fellipe Vieira , Arnhold Emmanuel , de Paula Fernanda Gomes , Mascarenhas Alessandra Gimenez , Mello Heloisa Helena de Carvalho 
Published: October 2023

Abstract
The objective was to evaluate the impact of stocking density on the productive and physiological parameters of Pangasius hypophthalmus throughout the production period. The experiment followed a completely randomized design with five treatments and four replications, resulting in a total of 20 experimental units. The densities tested were as follows: 30, 50, 70, 90, and 110 fish/m³, which corresponded to 15, 25, 35, 45, and 55 fish per box, respectively. The experimental period lasted 90 days. Stocking density did not influence feed conversion ratio, mortality, carcass yield, serum biochemical profile, or hematology. Body weight gain and feed intake responded linearly to the different densities. The limnological variables of temperature, pH, and dissolved oxygen were not affected, unlike nitrite (NO2), total ammonia (NH4+ + NH3), and non-ionized ammonia (NH3) levels, which rose linearly with increasing stocking density. In conclusion, it is advisable to consider stocking densities above 87 fish/m3 for the intensive cultivation of P. hypophthalmus in tropical climatic conditions.
A telephone follow-up management model for oral outpatient chemotherapy featuring concerted interventions by hospital and community pharmacists, and physicians
Authors: Makihara Reiko Ando , Hayashi Yoshikazu , Miki Akiko , Watanabe Noriko Watanabe , Sano Atsuhiko , Ohara Akane , Makino Yoshinori Makino , Sawada Yasufumi , Iwasa Satoru , Morizane Chigusa , Ueno Hideki , Okusaka Takuji , Hashimoto Hironobu 
Published: October 2023

Abstract
Purpose
Despite the critical importance of patients’ recognition and self-management of adverse drug effects (ADEs) in oral outpatient chemotherapy, continuous care and patient education in this field have not been fully explored in Japan. We conducted a prospective pilot study to examine the feasibility of a management model for oral outpatient chemotherapy.
Methods
The inclusion criterion was patients treated with S-1 alone or in combination with other agents. In the management model, patients underwent consultation with a hospital oncology pharmacist after a checkup with their physician, and were followed up by a community pharmacist on the telephone at least once a week until their next hospital visit. Both pharmacists focused on drug-related problems (e.g., obstacles to adherence, early ADEs), provided counseling, and informed the physician of any ADEs. The primary outcome was feasibility of the management model. The secondary outcomes were medication adherence, ADEs, and patient satisfaction.
Results
Among 23 eligible patients, 20 (with colorectal, pancreatic, or biliary cancer) agreed to participate. Of these, 14 were treated with S-1 monotherapy and 6 with S-1, irinotecan, and bevacizumab (SIRB) or S-1, oxaliplatin, and bevacizumab (SOX/BV). The completion rate of telephone follow-up was 100%. Mean adherence was 99.5% (range: 95.5–100.0). Community pharmacists identified 16 ADE cases during the treatment period. In a satisfaction questionnaire, 17/17 patients indicated that the hospital pharmacist consultation was useful, and 15/17 viewed the telephone follow-up as helpful.
Conclusions
Our new management model featuring concerted interventions enables continuous management for safe and effective oral outpatient chemotherapy.
Benchmarking of Methods for DNA Methylome Deconvolution
Authors: Thienpont Bernard , De Ridder Kobe , Che Huiwen , Leroy Kaat 
Published: October 2023

Abstract

Defining the number and abundance of different cell types in tissues is important for understanding disease mechanisms as well as for diagnostic and prognostic purposes. Typically, this is achieved by immunohistological analyses, cell sorting, or single-cell RNA-sequencing. Alternatively, cell-specific DNA methylome information can be leveraged to deconvolute cell fractions from a bulk DNA mixture. However, comprehensive benchmarking of deconvolution methods and modalities was not yet performed. Here we evaluated 13 deconvolution algorithms, developed either specifically for DNA methylome data or more generically. We assessed the performance of these algorithms, and the effect of normalization methods, while modelling variables that impact deconvolution performance, including cell abundance, cell type similarity, reference panel size, method for methylome profiling (array or sequencing), and technical variation. We observed differences in algorithm performance depending on each these variables, emphasizing the need for tailoring deconvolution analyses. The complexity of the reference, the number of marker loci and, for sequencing-based assays, the sequencing depth have a marked influence on performance. By developing handles to select the optimal analysis configuration, we provide valuable source of information for studies aiming to deconvolute array- or sequencing-based methylation data.