bluuebunny/crossref_metadata_2025_split huggingface dataset:
A human papillomavirus 16 E2-TopBP1 dependent SIRT1-p300 acetylation switch regulates mitotic viral and human protein levels
Authors: Prabhakar Apurva T. , James Claire D. , Youssef Aya H. , Hossain Reafa A. , Hill Ronald D. , Bristol Molly L. , Wang Xu , Dubey Aanchal , Morgan Iain M. 
Published: January 2024

AbstractAn interaction between human papillomavirus 16 (HPV16) E2 and the cellular proteins TopBP1 and BRD4 is required for E2 plasmid segregation function. The E2-TopBP1 interaction promotes increased mitotic E2 protein levels in U2OS and N/Tert-1 cells, as well as in human foreskin keratinocytes immortalized by HPV16 (HFK+HPV16). SIRT1 deacetylation reduces E2 protein stability and here we demonstrate that increased E2 acetylation occurs during mitosis in a TopBP1 interacting dependent manner, promoting E2 mitotic stabilization. p300 mediates E2 acetylation and acetylation is increased due to E2 switching off SIRT1 function during mitosis in a TopBP1 interacting dependent manner, confirmed by increased p53 stability and acetylation on lysine 382, a known target for SIRT1 deacetylation. SIRT1 can complex with E2 in growing cells but is unable to do so during mitosis due to the E2-TopBP1 interaction; SIRT1 is also unable to complex with p53 in mitotic E2 wild type cells but can complex with p53 outside of mitosis. E2 lysines 111 and 112 are highly conserved residues across all E2 proteins and we demonstrate that K111 hyper-acetylation occurs during mitosis, promoting E2 interaction with Topoisomerase 1 (Top1). We also demonstrate that K112 ubiquitination promotes E2 proteasomal degradation during mitosis. The results present a model in which the E2-TopBP1 complex inactivates SIRT1 during mitosis and E2 acetylation on K111 by p300 increases, promoting interaction with Top1 that protects K112 from ubiquitination and therefore E2 proteasomal degradation.ImportanceHuman papillomaviruses are causative agents in around 5% of all human cancers. While there are prophylactic vaccines that will significantly alleviate HPV disease burden on future generations, there are currently no anti-viral strategies available for the treatment of HPV cancers. To generate such reagents, we must understand more about the HPV life cycle, and in particular about viral-host interactions. Here we describe a novel mitotic complex generated by the HPV16 E2 protein interacting with the host protein TopBP1 that controls the function of the deacetylase SIRT1. The E2-TopBP1 interaction disrupts SIRT1 function during mitosis in order to enhance acetylation and stability of viral and host proteins. This novel complex is essential for the HPV16 life cycle and represents a novel anti-viral therapeutic target.
Elucidating ATP’s Role as Solubilizer of Biomolecular Aggregate
Authors: Sarkar Susmita , Gupta Saurabh , Mahato Chiranjit , Das Dibyendu , Mondal Jagannath 
Published: January 2024

AbstractProteins occurring in significantly high concentrations in cellular environments (over 100 mg/mL) and functioning in crowded cytoplasm, often face the prodigious challenges of aggregation which are the pathological hallmark of aging and are critically responsible for a wide spectrum of rising human diseases. Here we combine a joint-venture of complementary wet-lab experiment and molecular simulation to discern the potential ability of adenosine triphosphate (ATP) as solubilizer of protein aggregates. We show that ATP prevents both condensation of aggregation-prone intrinsically disordered protein Aβ40 and promotes dissolution of pre-formed aggregates. Computer simulation links ATP’s solubilizing role to its ability to modulate protein’s structural plasticity by unwinding protein conformation. We show that ATP is positioned as a superior biological solubilizer of protein aggregates over traditional chemical hydrotropes, potentially holding promises in therapeutic interventions in protein-aggregation related diseases. Going beyond its conventional activity as energy currency, the amphiphilic nature of ATP enables its protein-specific interaction that would enhance ATP’s efficiency in cellular processes.
Structural Basis of How MGME1 Processes DNA 5′ Ends to Maintain Mitochondrial Genome Integrity
Authors: Mao Eric Y.C. , Yen Han-Yi , Wu Chyuan-Chuan 
Published: January 2024

ABSTRACTMitochondrial genome maintenance exonuclease 1 (MGME1) helps to ensure mitochondrial DNA (mtDNA) integrity by serving as an ancillary 5′ exonuclease for DNA polymerase γ. Curiously, MGME1 exhibits unique bidirectionalityin vitro, being capable of degrading DNA from either the 5′ or 3′ end. The structural basis of this bidirectionally and, particularly, how it processes DNA from the 5′ end to assist in mtDNA maintenance remains unclear. Here, we present a crystal structure of human MGME1 in complex with a 5′-overhang DNA, revealing that MGME1 functions as a rigid DNA clamp equipped with a single-strand-(ss)-selective arch, allowing it to slide on single-stranded DNA in either the 5′-to-3′ or 3′-to-5′ direction. Using a nuclease activity assay, we have dissected the structural basis of MGME1-derived DNA cleavage patterns in which the arch serves as a ruler to determine the cleavage site. We also reveal that MGME1 displays partial DNA-unwinding ability that helps it to better resolve 5′-DNA flaps, providing insights into MGME1-mediated 5′-end processing of nascent mtDNA. Our study builds on previously solved MGME1-DNA complex structures, finally providing the comprehensive functional mechanism of this bidirectional, ss-specific exonuclease.
Spatiotemporal visualization of DNA replication by click chemistry reveals bubbling of viral DNA in virion formation
Authors: Gomez-Gonzalez Alfonso , Burkhardt Patricia , Bauer Michael , Loehr Morten O. , Luedtke Nathan W. , Greber Urs F. 
Published: January 2024

AbstractThe organisation of human chromosomes reversibly changes in cell division, and irreversibly in apoptosis or erythropoiesis by DNA condensation and fragmentation processes. Yet, how viral replication in the nucleus affects host and viral chromatin organisation remains poorly understood. Here we used dual-color click chemistry to image human adenovirus DNA replication, demonstrating host chromatin compaction during active expansion of the viral replication compartment (VRC). Early-replicated viral DNA (vDNA) segregated from VRC and lost phospho-serine5-RNA Pol-II and viral DNA-binding protein (DBP), while late-replicated vDNA retained active RNA Pol-II, besides viral RNA-splicing and DNA-packaging proteins. Depending on replication and the assembly protein 52K, the late-stage VRCs gave rise to progeny by droplet formation of vDNA with GFP-tagged virion protein V into 52K biomolecular condensates. The study reveals distinct functions of early and late-replicated vDNA and provides insight into active and passive liquid phase separated zones conducive to selective genome packaging of nascent virions.
Long-horizon associative learning explains human sensitivity to statistical and network structures in auditory sequences
Authors: Benjamin Lucas , Sablé-Meyer Mathias , Fló Ana , Dehaene-Lambertz Ghislaine , Roumi Fosca Al 
Published: January 2024

AbstractNetworks are a useful mathematical tool for capturing the complexity of the world. In a previous behavioral study, we showed that human adults were sensitive to the high-level network structure underlying auditory sequences, even when presented with incomplete information. Their performance was best explained by a mathematical model compatible with associative learning principles, based on the integration of the transition probabilities between adjacent and non-adjacent elements with a memory decay. In the present study, we explored the neural correlates of this hypothesis via magnetoencephalography (MEG). Participants passively listened to sequences of tones organized in a sparse community network structure comprising two communities. An early difference (~150 ms) was observed in the brain responses to tone transitions with similar transition probability but occurring either within or between communities. This result implies a rapid and automatic encoding of the sequence structure. Using time-resolved decoding, we estimated the duration and overlap of the representation of each tone. The decoding performance exhibited exponential decay, resulting in a significant overlap between the representations of successive tones. Based on this extended decay profile, we estimated a long-horizon associative learning novelty index for each transition and found a correlation of this measure with the MEG signal. Overall, our study sheds light on the neural mechanisms underlying human sensitivity to network structures and highlights the potential role of Hebbian-like mechanisms in supporting learning at various temporal scales.
Electron-positron, parton-parton, and photon-photon production of
τ
-lepton pairs: Anomalous magnetic and electric dipole moments spin effects
Authors: Banerjee Sw. , Korchin A. Yu. , Richter-Was E. , Was Z. 
Published: January 2024

Anomalous contributions to the electric and magnetic dipole moments of the τ lepton from new physics scenarios have brought renewed interest in the development of new charge-parity violating signatures in τ-pair production at Belle II energies, and also at higher energies of the Large Hadron Collider and the Future Circular Collider. In this paper, we discuss the effects of spin correlations, including transverse degrees of freedom, in the τ-pair production and decay. These studies include calculating analytical formulas, obtaining numerical results, and building semirealistic observables sensitive to the transverse spin correlations induced by the dipole moments of the τ lepton. The effects of such anomalous contributions to the dipole moments are introduced on top of precision simulations of e−e+→τ−τ+, qq¯→τ−τ+ and γγ→τ−τ+ processes, involving multibody final states. The τ decays are simulated along with radiative corrections, in particular electroweak box contributions of WW and ZZ exchanges are taken into account. Respective extensions of the Standard Model amplitudes and the reweighting algorithms are implemented into the Monte Carlo, which is used to simulate τ-pair production in e−e+ collisions, and the aupinner program, which is used to reweight events with τ pair produced in pp collisions.

Published by the American Physical Society
2024
How do green intellectual and co-creational capitals drive artificial intelligence innovation and green innovation in start-ups?
Authors: Almansour Mohammed 
Published: January 2024

PurposeThis research examines the relationship between the green version of intellectual capital (IC) (measured through green versions of human, structural and relational capitals (GHC, GSC and GRC)), co-creational capital (CC), green innovation (GI), technological innovation (TI) (measured through artificial intelligence) and start-up competitive advantage (SCA).Design/methodology/approachAn online questionnaire collected data from 275 participants. To test the hypotheses, the data were analyzed using SmartPLS.FindingsThe results confirmed the positive influence of GSC and CC on TI and GI, GRC with GI and that of GI and TI with SCA. The results also reveal that IC can influence innovation and describe how innovation can drive the competitive advantage (CA) of start-ups.Research limitations/implicationsThis self-report study examines the associations by collecting data at one point in time, which results in methodological limitations regarding the generalization of the results. The second limitation is that the findings are limited to start-ups.Originality/valueThis research work examined a model that combined three components of green IC, customer capital, two forms of innovation and CA. These associations have not been previously examined yet can provide useful insight into what drives green and TIs and how they further influence competitiveness. This study provides unique inferences that improve the value of the literature on IC and innovation, using start-ups as context.
Mediating effect of absorptive capacity on the relationship between knowledge sharing and entrepreneurial orientation and the moderating role of opportunity recognition
Authors: Makhloufi Lahcene , Laghouag Abderrazak Ahmed , Ali Sahli Alhussain 
Published: January 2024

Purpose
Knowledge sharing enables a firm’s absorptive capacity to reconfigure its dynamic capabilities to sense, track and recognise embryonic business opportunities. Entrepreneurial opportunity recognition triggers entrepreneurs to invest in and upgrade their knowledge practices to improve entrepreneurial performance. This study aims to examine the relationship between knowledge sharing and absorptive capacity on entrepreneurial orientation as well as the moderating effect of opportunity recognition and the mediating effect of absorptive capacity.

Design/methodology/approach
A quantitative cross-sectional research design was applied to data from Algerian small and medium-sized enterprise managers in middle- to top-management positions. The final analysis using smart PLS included 246 respondents.

Findings
Knowledge sharing positively influenced entrepreneurial orientation and absorptive capacity. In addition, the findings reveal that entrepreneurial orientation positively influences entrepreneurial performance, and opportunity recognition strengthens the relationship between entrepreneurial orientation and entrepreneurial performance. Entrepreneurial orientation and absorptive capacity have partial mediating effects.

Practical implications
This study focuses on how firms create and share developed knowledge to enable absorptive capacity to fit a level of entrepreneurial orientation. This study validates the vital impact of opportunity recognition as a leading motivation for entrepreneurial orientation. The results highlight an important area of research and suggest that firms should focus on knowledge sharing to boost entrepreneurial outcomes.

Originality/value
The notions of entrepreneurship, absorptive capacity and knowledge sharing are extended by building on dynamic capability theory. Knowledge sharing increases absorptive capacity, which drives superior entrepreneurial orientation outcomes.
Relationship building through informal gatherings and technology integrations: a case study
Authors: Gala Bhakti , Reyes Vanessa , Seifi Leili , Lamba Manika 
Published: January 2024

Purpose
This case study aims to explore how to provide an informal space to connect members socially, informally and with peers, to provide opportunities for collaboration and informal conversation. The purpose is to analyze the use of technology to promote collaborations and networking within online professional settings.

Design/methodology/approach
In this case study, the authors particularly examine the initiatives as they contribute to the organization, which allows us to understand the interactions between information technology-related tools and online communication.

Findings
Successful engagement in informal gatherings was initiated through the planning and implementation with a focus on the following: thematic meetings, time management and participation.

Originality/value
The case study presents an analysis of the use of technology integration into the planning processes that were undertaken by a group of members to enhance informal gatherings for relationship building within a global community of members during the COVID-19 pandemic. The authors suggest ways of engaging users that could be used as a model for organizations alike.
Research across disciplines: influence of human resource management practices
Authors: Martín-Alcázar Fernando , Ruiz-Martínez Marta , Sánchez-Gardey Gonzalo 
Published: January 2024

PurposeThis study aims to examine the connection between scholars' research performance and the multidisciplinary nature of their collaborative research. Furthermore, in response to mixed results regarding the effects of multidisciplinarity on research performance, this study explores how human resource management (HRM) practices may moderate this link.Design/methodology/approachThe authors built a model based on the theoretical arguments and empirical evidence found in the review of diversity and HRM literature. The authors also performed a quantitative study based on a sample of scholars in the field of management. Different econometric estimations were used to test the proposed model.FindingsThe results of this empirical analysis suggest that multidisciplinary research has a non-linear effect on research performance. Certain HRM practices, such as development and collaboration, moderated the curvilinear relationship between multidisciplinarity and performance, displacing the optimum to allow higher performance at higher levels of multidisciplinary research.Originality/valueThe paper provides advances on previous works studying the curvilinear relationship between multidisciplinarity and the researchers' performance, confirming that multidisciplinarity is beneficial up to a threshold beyond which these benefits are attenuated. In addition, the findings shed light on important issues related to team-oriented HRM practices associated with the outcomes of multidisciplinary research.
Management learning in public healthcare during pandemics
Authors: Rosenbäck Ritva , Svensson Ann 
Published: January 2024

Purpose
This study aims to explore the management learning during a long-term crisis like a pandemic. The paper addresses both what health-care managers have learnt during the COVID-19 pandemic and how the management learning is characterized.

Design/methodology/approach
The paper is based on a qualitative case study carried out during the COVID-19 pandemic at two different public hospitals in Sweden. The study, conducted with semi-structured interviews, applies a combination of within-case analysis and cross-case comparison. The data were analyzed using thematic deductive analysis with the themes, i.e. sensemaking, decision-making and meaning-making.

Findings
The COVID-19 pandemic was characterized by uncertainty and a need for continuous learning among the managers at the case hospitals. The learning process that arose was circular in nature, wherein trust played a crucial role in facilitating the flow of information and enabling the managers to get a good sense of the situation. This, in turn, allowed the managers to make decisions meaningful for the organization, which improved the trust for the managers. This circular process was iterated with higher frequency than usual and was a prerequisite for the managers’ learning. The practical implications are that a combined management with hierarchical and distributed management that uses the normal decision routes seems to be the most successful management method in a prolonged crisis as a pandemic.

Practical implications
The gained knowledge can benefit hospital organizations, be used in crisis education and to develop regional contingency plans for pandemics.

Originality/value
This study has explored learning during the COVID-19 pandemic and found a circular process, “the management learning wheel,” which supports management learning in prolonged crises.
Connect with care: informal knowledge protection practices to enhance knowledge sharing in networks of organizations
Authors: Thalmann Stefan , Maier Ronald , Remus Ulrich , Manhart Markus 
Published: January 2024

Purpose
This paper aims to clarify how organizations manage their participation in networks to share and jointly create knowledge but also risk unwanted knowledge spillovers at the same time. As formal governance, trust and observation are less applicable in informal networks, the authors need to understand how members address the need to protect knowledge by informal practices. The study aims to investigate how the application of knowledge protection practices affects knowledge sharing in networks. The insights are relevant for organizational and network management to control knowledge risks but harvest the benefits of network engagement.

Design/methodology/approach
The authors opted for an exploratory study based on 60 semi-structured interviews with members of 10 networks. In two rounds, network managers, representatives and members of the networks were interviewed. The second round of interviews was used to validate the intermediate findings. The data were complemented by documentary analysis, including network descriptions.

Findings
Through analyzing and building on the theory of psychological contracts, two informal practices of knowledge protection were found in networks of organizations: exclude crucial topics and share on selected topics and exclude details and share a selected level of detail. The authors explored how these two practices are enacted in networks of organizations with psychological contracts.

Originality/value
Counter to intuition that the protection of knowledge can be strengthened only at the expense of knowledge sharing and vice versa, networks benefitted from more focused and increased knowledge sharing while reducing the risk of losing competitive knowledge by performing these knowledge protection practices.
Impact of emotional intelligence on knowledge hiding behaviour of teachers: a moderated mediation analysis
Authors: Shailja Shailja , Kumari Patiraj , Singla Himanshu 
Published: January 2024

Purpose
Given the scarcity of research exploring the influence of emotional dimensions on knowledge hiding (KH), this paper aims to examine how various facets of emotional intelligence (EI) affect teachers’ tendencies to hide knowledge. Additionally, the study delves into the mediating influence of organizational cynicism (OC) and explores the moderating role of servant leadership (SL) in this context.

Design/methodology/approach
The non-probability sampling method was used to gather data from 542 university teachers teaching in India. Structural equation modelling using Smart-PLS has been used to test the hypothesis.

Findings
The study revealed that EI negatively influences KH. Additionally, OC mediates the relationship between EI and KH. Furthermore, SL moderates the relationship between OC and KH.

Originality/value
As per the authors’ knowledge, the present study makes an additional contribution by investigating the mediating role of OC in the relationship between EI and KH. Moreover, it also investigates the moderating role of SL in the relationship between OC and KH.
Contested ‘commune rurales’: Decentralisation and the (violent) struggle for public authority in the Democratic Republic of Congo
Authors: Büscher Karen , Perazzone Stephanie , Cuvelier Jeroen , Lumbu Stephane , Rwakira Espoir , Bulambo Paul , Mvano Yabauma Chrispin , Muzalia Godefroid 
Published: January 2024

AbstractThis article explores how decentralisation policy and specifically the establishment of communes rurales in DR Congo turned into a profoundly destabilising juncture, shaking existing governance arrangements. In particular, we examine how this has led to a reshuffle of power and a renegotiation of public authoritiy. By analysing the impact of decentralisation on the construction of and competition over public authority in three Congolese towns – Rubaya, Minembwe and Fungurume – we demonstrate how decentralisation is deeply politicised, with conflicting governance actors mobilising their power in an attempt to secure their claim to public authority. We argue that the establishment of communes rurales in eastern and southeastern DRC should be therefore understood as a strongly destabilising moment, changing the access of governance actors to resources and repertoires from which they build and legitimise their public authority. Depending on the specific context of the local political arena and its entanglements with larger struggles for power and control, this destabilising moment bears the potential for (violent) conflict. As such, we conclude that decentralisation has failed to live up to its promises of stability and peace while generating new sets of political fault lines and a re‐activation of (violent) conflict.