In Vivo Studies on the Capacity of Greenlid Bio-traps (Glbt) to Minimize Overall Population of Aedes Aegypti/Albopictus in Dengue Risk Areas (Faisalabad)
Authors: BUKHARI SYED AOUN TAQI , Akram Waseem , Mehmood Iqra , Tariq Muhammad , Ahmad Shahbaz , Shehzad Muhammad 
Published: December 2023

Abstract
Background: Mosquito-borne diseases are characterized by their specific biological transmission and these are able to imbibe the viruses on infected host. Tremendously affecting mosquito is Aedes and its species which are responsible as a vector for the diseases like malarial filariasis, dengue, chikungunya, Japanese encephalitis, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). With the upcoming health problems dengue virus is of great importance and is enzootic in humid and semitropical countries including Pakistan. Efforts have been made to fight with the proliferating disease to minimize the victims by controlling the population of Aedes. With the new era and the increase in epidemiology of the diseases the need of the hour is to incorporate some of the best and viable methods that result in bringing their number to the lowest and ultimately eliminating the Aedes population.
Results: The present study was carried out to check the capacity of Greenlid Bio-traps (GLBT) to minimize the overall population of Aedes in dengue risk areas under field trials. GLBT are internally coated with permethrin which is used to inhibit adult formation and is able to kill the adult female within 5 minutes after it came into contact with it. GLBT were placed in four different locations. Adults emerging from each of the GLBT were monitored and was compared with adults emerging from controlled conditions. The duration for the completion of the study was about 4-5 months. Data was collected on weekly basis. Late 3rd and 4th instar larvae of Cx. quinquefasciatus and Ae. aegypti were used as test insects. Pupa collection was done weekly to see adult emergence. Results of the study showed non-significant results and showed that the biotraps were effective in the open areas of school as compared to the houses where the traps were placed. Mosquito population was also found with the number of larvae, pupa and adults. These bio-traps are effective to a little extent against different mature and immature stages of mosquito species.
Conclusion: All of the traps mostly degraded or damaged from the bottom, not fully degraded. Most of the traps degraded at higher temperature of 45 C. Results for adult mortality, adult emergence, larval formation, larval mortality, pupal formation and deformation was non-significant. 2 trials were done in a time period of about 4 months. Somehow significant results were obtained and shows the non-significant difference in the study. There also the presence of adults, larvae and pupal formation in the UC: 3 where the traps were placed in an open environment in the schools to large extent because of the presence of the suitable temperature and the favourable breeding spots present there. It is concluded that the traps were less effective in the houses as compared with the schools areas where there present the mosquitoes population.