bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial distribution of mutations in voltage-gated sodium channel genes of Culex pipiens pallens/Culex pipiens quinquefasciatus in China
Authors: Li Wenyu , Ma Delong , Mu Qunzheng , Zhou Xinxin , Hua Dongdong , Zhao Chunchun , Zhao Qiyong , Wang Jun , Meng Fengxia 
Published: October 2023

Abstract
Background
Culex pipiens pallens and Culex pipiens quinquefasciatus are the main species of bloodsucking mosquitoes in China. The aim of this study is to understand the current situation of VGSC genes of Cx. pipiens pallens and Cx. pipiens quinquefasciarus in China and the impact of pyrethroid insecticides on the long-term evolution of mosquito populations, providing a basis for scientific prevention and control.
Methods
Study 28 geographic populations in 22 provinces. Partial fragments of the voltage-gated sodium channel (VGSC) gene on the nerve cell membrane were amplified by PCR. The mutation, mutation frequency and phylogenetic were analyzed after sequencing.
Results
There were 6 alleles and 6 genotypes at the L1014 locus, which were wild-type TTA / L and CTA / L, mutant TTT / F, TTC / F, TCT / S and TCA / S. The proportion of homozygous L/L and mutant homozygous F/F in genotype is similar, which are more than 30.00% The geographic populations with frequency less than 20.00% are mainly concentrated in the north of 38 ° N, and the geographic populations with frequency more than 80.00% in the south of 30 ° N. frequency increases with the decrease of latitude, and frequency has urban tendency. mutation is correlated with introns. The mutant allele TCA / S has only one intron, TTT / F has three introns, and the wild allele TTA / L has 17 introns.
Conclusions
The allelic genotype of Culex pipiens pallens/Culex pipiens quinquefasciatus in China is diversified, and the resistance of Culex pipiens pallens/Culex pipiens quinquefasciatus in most areas has been developed, and the degree of resistance is regional. gene has a polymorphism in its adjacent downstream intron and is related to mutation.
Cost-competitiveness of distributed ammonia production
for the global fertilizer industry
Authors: Tonelli Davide , Rosa Lorenzo , Gabrielli Paolo , Parente Alessandro , Contino Francesco 
Published: October 2023

Abstract

The production of ammonia as a precursor to nitrogen fertilizers contributes to about 1% of global CO2 emissions and consumes about 2% of global energy. Current ammonia and fertilizers production is centralized in large facilities, from which fertilizers are then transported to croplands, and use fossil fuels as a feedstock for the Haber-Bosch process. The reliance of global food production on trading fertilizers and the volatile fossil fuel prices exposes fertilizers to supply shocks. These factors, in addition to the need to achieve net-zero greenhouse gas emissions, prompts a restructuring in the ammonia industry. An alternative approach consists in producing ammonia in small modular facilities via electric Haber-Bosch or direct electrocatalytic reduction. Here, we examine the cost-competitiveness of a new ammonia industry where low-carbon ammonia production is co-located at the cropland level. Techno-economic parameters and biophysical limits are considered for production in 2020 and for projected production in 2030 and 2050. Considering the median historical market price of ammonia in the 2000-2023 period, our findings indicate that cost-competitiveness cannot be achieved. However, by 2030, distributed electric Haber-Bosch could supply 52% (53 Mt/y) and electrocatalysis 7% (7 Mt/y) of global ammonia demand at a lower cost than the peak historical market price, increasing to 71% (73 Mt/y) by 2050 for both the technologies.
TNF-a-induced laminin-332 promotes an atherogenic endothelial phenotype
Authors: Hayderi Assim , Zegeye Mulugeta Melkie , Meydan Sare , Sirsjö Allan , Kumawat Ashok Kumar , Ljungberg Liza Ulrika 
Published: October 2023

Abstract
Background:Laminins are key components of the basement membranes separating endothelial cells from the underlying tissues. They form complex networks of fibers within the basement membrane to provide structural support and regulate the behavior and function of surrounding cells. In mammals, the products of the five alpha, four beta and three gamma laminin genes are combined to generate 16-18 laminin isoforms, which under physiological circumstances confer some tissue specificity, with alpha 4- and alpha 5-containing isoforms being considered vascular-specific. During inflammation, however, the expression pattern of laminin genes may change, which in turn may affect the behavior of surrounding cells.
Objectives: We aimed to investigate the impact of tumor necrosis factor alpha (TNF-a) on laminin gene expression in endothelial cells and examine the impact of TNF-a-induced laminin (laminin-332 or LN332) on endothelial cells’ behavior.
Results:TNF-a induces LN332-encoding genes in endothelial cells. Endothelial cells cultured on LN332 display irregular shape and appear loosely connected. These cells acquire lower expression of tight junction protein, claudin-5, elevated expression of leukocyte adhesion molecules and enhanced secretion of chemokines. In addition, cells cultured on LN332 are more adhesive to leukocytes and supernatant from these cells is more attractive for primary monocytes in vitro. Notably, LN332-encoding genes are elevated in human atherosclerotic lesions and correlate with the expression of TNF-a.
Conclusions: We demonstrate that TNF-a-induced upregulation of LN332 promotes a pro-atherogenic endothelial phenotype. Elevated levels of LN332-encoding genes in atherosclerotic lesions, correlating with TNF-a, suggest a potential role for LN332 in the pathophysiology of atherosclerosis.
A Modular Approach to Design an Experimental Framework for Resource Management Research
Authors: Pons Lucia , Petit Salvador , Pons Julio , Gómez María E. , Sahuquillo Julio 
Published: October 2023

Abstract
Research on resource management focuses on optimizing system performance and energy efficiency by distributing shared resources like processor cores, caches, and main memory among competing applications. This research spans a wide range of applications, including those from high-performance computing, machine learning, and mobile computing. Existing research frameworks often simplify research by concentrating on specific characteristics, such as the architecture of the computing nodes, resource monitoring, and representative workloads. For instance, this is typically the case with cloud systems, which introduce additional complexity regarding hardware and software requirements. To avoid this complexity during research, experimental frameworks are being developed. Nevertheless, proposed frameworks often fail regarding the types of nodes included, virtualization support, and management of critical shared resources.
This paper presents Stratus, an experimental framework that overcomes these limitations. Stratus includes different types of nodes, a comprehensive virtualization stack, and the ability to partition the major shared resources of the system. Even though Stratus was originally conceived to perform cloud research, its modular design allows Stratus to be extended, broadening its research use on different computing domains and platforms, matching the complexity of nowadays cloud environments.
The diet of the giant anteater, Myrmecophaga tridactyla Linnaeus 1758, in the Cerrado of Mato Grosso do Sul, Brazil
Authors: SILVA TAINARA SANTANA GALVAO DA , ATTIAS Nina , NASCIMENTO Natalia Teixeira , TIBCHERANI Mariáh , DESBIEZ Arnaud Léonard Jean 
Published: October 2023

Abstract
Relationships between species and their habitat are strongly related to their feeding habits making its description essential for understanding the species’ biology and plan their conservation. This study describes the diet of giant anteaters (Myrmecophaga tridactyla), a specialist insectivorous mammal, and evaluates potential sex-related intraspecific variation. We analyzed fecal matter from live individuals, and stomach and feces content from road-kills (N = 29 individuals) collected between 2018 and 2020 in the Cerrado savanna of Mato Grosso do Sul, Brazil. Anteater’s diet was described by the relative frequency (RF; proportion of the sample mass represented by the prey item) and the frequency of occurrence (FO; proportion of samples in which the item was detected) of each prey item, which was classified to the lowest taxonomic level possible. The diet was composed of 29 morphotypes of ants (Formicidae), 14 morphotypes of termites (Blattodea) and grass seeds (Poacea). They fed primarily on ants (FO = 100%; RF = 29.19%) and termites (FO = 93.10%; RF = 69.85%), with no significant differences between sexes (F = 1.0773; p = 0.37). Termites of the genus Syntermes (RF = 69.59%) represented most of the dietary sample mass, and ants of the genus Pheidole represented the most frequent item found in the samples (FO = 93.10%). M. tridactyla is a myrmecophagous specialist that consumes a great richness of prey species associated with multiple habitats. Hence, the preservation of the natural heterogeneity of the Cerrado landscape and their habitats is essential for the conservation of this vulnerable species.
Wear behaviour of SiC-Reinforced Metal Matrix Composites FeCrNiCuMo High Entropy Alloy Processed Through Powder Metallurgy
Authors: T Ramprabhu , P Vignesh , S Krishnakumar , GS Anand , CM Bhuvaneswari , SS Kale 
Published: October 2023

Abstract
A series of FeCrNiCuMo high-entropy alloys with different SiC (x = 10 and 15% wt. %) contents were designed, and their microstructure and tribological behaviour were investigated. The tribological behaviour and wear resistance of the composites were evaluated at a range of sliding speeds (20, 30, and 40 ms− 1) in a brake dynamometer for brake friction material applications. According to the estimates of volume loss, wear rate, and friction coefficient, the possible wear mechanisms were suggested in all cases. Additionally, SEM/EDS analysis was conducted on the worn surfaces and debris. With the increase in sliding speed, the wear rate increases due to the increased intensity of abrasive wear, oxidation wear, and plastic deformation-assisted wear. The materials possessed excellent braking performance and wear resistance. The values of average coefficient friction under A1 (20ms− 1), A2 (30ms− 1), and A3 (40ms− 1) sliding conditions were 0.23, 0.29, and 0.34, respectively. In comparison to the A3, the A1 exhibits a lower volume wear loss. As the sliding speed change, adhesive wear and abrasive wear become more prevalent, and fatigue wear also occurs.
A digestive enzyme and herbal dietary supplement reduces bloating in a single use in healthy adults: A randomized, placebo-controlled, cross over study
Authors: Martin-Biggers Jennifer 
Published: October 2023

Abstract
Background
Healthy individuals with no diagnosed gastrointestinal condition may suffer from occasional meal-related bloating, gassiness and discomfort that negatively affects quality of life. This study examined the effect and tolerability of a digestive enzyme and herbal dietary supplement to improve meal-related bloating, gassiness and discomfort.
Methods
Adults aged 18 to 45 (n = 25) with self-reported bloating or abdominal distension at least once per day after a meal and no preexisting condition or medication use that may cause bloating were enrolled. After obtaining informed consent, fasted participants were randomized to consume either placebo or test product and consume a test meal. Waist circumference was measured at baseline, and again with surveys at 30- and 90-minutes post meal. Survey questionnaires asked participants to rate the momentary severity of bloating, distended stomach, gas, and indigestion on a 4-point scale (severe, moderate, mild, absent). Following a 1-week washout, participants repeated study procedures with the other study product. Descriptive statistics were used for continuous and categorical values, with independent sample t-tests conducted to examine between group differences and paired t-tests to examine differences in waist circumference scores.
Results
Participants (n = 20) experienced 58% less abdominal distension as assessed by the waist circumference measure at 30 minutes (0.93 cm vs 1.50 cm, p = 0.04) and 68% less at 90 minutes with the test product compared to the placebo (0.94 cm vs 2.12 cm, p = 0.007). Overall, 80% of participants had less distension with the test product compared to the placebo. No significant differences were noted between questions assessing momentary bloating, distended stomach, gas nor indigestion. There was a trend to more strongly agree the test product was effective for overall feelings of bloating, stomach discomfort and distension, as well as indigestion, with 65% of participants reporting less stomach discomfort and 55% reporting feeling less gassy than normal with the test product. No product-related adverse events or discomfort was reported.
Conclusions
A dietary supplement containing a blend of digestive enzymes and herbal ingredients was well tolerated and effective at reducing post-meal abdominal distension compared to placebo in healthy subjects.
Trial Registration:
ClinicalTrials.gov, NCT05520411, Submitted August 26, 2022
A Novel Trusted Hardware-based Scalable Security Framework for IoT Edge Devices
Authors: Khan Mohd , Hatami Mohsen , Zhao Wenfeng , Chen Yu 
Published: October 2023

Abstract

The Internet of Things (IoT) devices are pervasively deployed and embedded into our daily lives. The massive assimilation of IoT devices over several years has given rise to smart cities, smart factories, smart farms, etc. While IoTs are capable of collecting immense user and/or environmental information, the lack of adequate security measures is among the major concerns about information security and privacy. With the ever-increasing presence of IoTs, information flow between IoT devices in a single or multi-layered network is becoming increasingly vulnerable to attacks. Although several software-based solutions have been developed over the years that improve the security of resource-constrained IoT devices, there are limited efforts on hardware-oriented IoT security solutions. This paper proposes a Trusted HArdware-based Scalable Secure Architecture (THASSA) for IoT Edge systems. Leveraging standard cryptographic algorithms, THASSA provides a secure mechanism for data transfer and off-loads cryptography operations to the trusted hardware. The modularized THASSA architecture allows devices to be added or removed without user intervention. Also, THASSA could be scaled to multiple subsystems using multiple trusted hardware to form IoT clusters with secure data path(s) for mission-critical applications. An experimental study has verified the correctness and effectiveness of THASSA.
Using a Social-Ecological Framework to Examine the Cognitive Development of Elementary School Children in the U.S.
Authors: Park Hyejoon , McCormick Melinda , Guo Siying , Liu Jin , Park Ju Hong 
Published: October 2023

Abstract

This study examined the cognitive areas of elementary school children in terms of applying significant and identifiable factors within a social-ecological framework. By studying the association between social-ecological factors and children’s cognitive areas, we observed which factors are most salient to associate with children’s cognitive outcomes. We used the 6thwave (9 years old) of the U.S. Fragile Family and Child Wellbeing Study. With a sample size of 1722, we used multiple regression analyses of the relationship between individual, family, and school/community levels and children’s verbal skills (PPVT Ⅲ), reading comprehension (WJ-PC), digital span, and math problems (WJ-AP). We found that children in special education schools showed lower scores in all cognitive areas than children in regular public schools; the school environment was associated with verbal skills, math skills, and reading comprehension; mothers’ educational levels were significantly associated with verbal skills, digital span, and reading comprehension; and children’s race was associated with verbal skills. To improve schoolers’ cognitive areas, policy-makers in education and educators should be concerned about the quality of schools and their environment. Special education should be carefully reviewed to address the specific learning needs of students and their developmental areas. Parenting education could be beneficial for parents with lower education.
NNMT involving with gastric adenocarcinoma immune infiltration by driving amino acids metabolism
Authors: Lu Jianyong , Gui Wenliang , Tang Shen , Shi Qianqian , Wang Xinhang , Huang Liyuan , Shen Yinghui , Yang Shuting , Xiang Junni , Yuan Jianglang , Mo Jiao , Kong Xingxing , Huang Mingwei , Li Xiyi , Lu Cailing 
Published: October 2023

Abstract
Objectives
Gastric cancer is a malignant tumor with high incidence rate and high mortality. Gastric adenocarcinoma (STAD) is the main pathological type of gastric cancer. Nicotinamide N-methyltransferase (NNMT) is a methylation enzyme that primarily participates in the metabolism of nicotinamide (NAM) in the body, which is highly expressed in many solid tumor tissues and is associated with cancer progression and tumor microenvironment. NNMT is a hot spotlight in the development of STAD.
Methods
Bioinformatics data Basing on the Cancer Genome Atlas (TCGA) was withdrawn and analyzed to explore the character of NNMT in STAD. Tissues from clinical patient specimens were collected and conducted Western blot and immunohistochemistry to explore the localization and expression of NNMT in cancer tissues and adjacent normal tissues of STAD patients. The profiles of amino acids in cancer tissues and adjacent normal tissues of STAD patients were compared. The relationship between of NNMT and genes to differential amino acids was further mapped.
Results
Bioinformatics analysis from the TCGA database revealed that NNMT is highly expressed in cancer tissues of STAD patients and influences tumor immune infiltration. The outcome from clinical specimen confirmed that both the NNMT mRNA and protein expression was significantly increased in cancer tissues of STAD patients compared to adjacent normal tissues. 28 differential amino acids, including alanine, leucine, aspartic acid, and glutamic acid etc. 10 differential amino acid metabolic enzymes, including GPX3, GAMT, AMD1, and ODC1 etc. were associated with NNMT. Clinical specimens confirmed a negative correlation between GPX3, GAMT, and AMD1 mRNA expression and NNMT.
Conclusion
NNMT is highly expressed in STAD tissues and may drive amino acid metabolites changes by their enzymes, thus influence STAD immune infiltration and tumorigenesis.
Vietnam Spam Review Detection using Graph Reinforcement Learning
Authors: Nguyen Van-Trung , Vo Van-Khanh , Truong Hai-Bang , Le Than , Nguyen Thuy-Dung , Pham Van-Huy , Nguyen Trung 
Published: October 2023

Abstract
With the proliferation of social media platforms, especially commercial platforms today, and the ease of access to internet shopping, it is difficult to determine which products are good for clients based on reviews from users. Numerous successful innovations in the field of spam product review detection have been made. This study focuses on the creation of an efficient method for Vietnam spam product review detection using cutting-edge language models in addition to Vietnam-related work. For feature extraction, we utilize PhoBERT, a cutting-edge pretrained model for Vietnamese language processing. The downstream task model will be followed by the Graph Reinforcement Learning architecture, which has had many successes recently. Our proposed GRL model tries to capture text reviews into graph representations and utilize passing mechanisms for leveraging the product description as a global message passing to the review. By combining with the RL framework, we can construct the modulator, which controls the amount of information passing from the product description and gives out the best outcomes in the long term and outperformance compared to traditional methods.
The localization and incidence of metoptic canal and Warwick’s foramen in adult skulls from West Anatolia
Authors: Aksu Funda , Karabekir Selim , Gocmen-Karabekir Nuket 
Published: October 2023

Abstract
Purpose
Metoptic canal (MC) is located between the superior orbital fissure and the optic canal. Warwick’s foramen (WF) is viewed on the inner side of the orbit together with foramen rotundum and superior orbital fissure. The aim of the present study was to determine the presence and incidence of MC and WF and to examine their morphometric characteristics in a sample of Turkish skulls from West Anatolia.
Methods
A total of 153 dried human skulls and 11 dried skull bases from a West Anatolian Turkish population collected at the Anatomy Department of Dokuz Eylul University were examined. Presence and incidence of the MC and of WF were determined. The distance between reference points and the structures examined within the orbit were measured using a caliper. Student's t test was used for comparisons between the right and left sided measurements. SPSS 22.0 software pack was used for statistical analysis.
Results
MC was detected in 10 of the 153 dry human skulls: 4 (2.6%) on the right side, 5 (3.2%) on the left side, and one (0.06%) bilaterally. There were no statistically significant differences between right and left side measurements (p > 0.05). WF was observed in 2 (18.2%) of the 11 dry skulls, and both were on the left.
Conclusion
The relationships between MC, WF and other anatomical structures may be of clinical importance in surgical operations involving the orbit.
Unveiling nature’s beat: analyzing the acoustics of an aquatic bug in relation to urban sounds
Authors: Farfán-Beltrán Manuel Edday , Cano-Santana Zenon , Córdoba-Aguilar Alex 
Published: October 2023

Abstract

Anthropophony is a prominent component of the soundscapes worldwide and is known to affect biota. Although responses to antropophony in terrestrial and marine animals have been relatively well studied, there is still a lack of knowledge regarding freshwater biota. In fact, freshwater taxa are possibly among the most threatened groups and so unveiling the reasons of threat is key. We investigated the low-frequency sounds - usually associated with anthropophony - on the stridulation of Krizousacorixa femorata, a common water bug in Mexico City. Using aerial recordings, we estimated the Normalized Difference Soundscape Index (NDSI) to characterize soundscapes in six city sites throughout a year. Also, we obtained the peak frequency (Pf) through underwater recordings. By last, we evaluated the relationships between the soundscapes and the Pf through a mixed linear model. There was a negative relation between NDSI and Pf, so there are higher-pitched stridulations at sites with dominant anthropophony. We cannot discard other factors that contribute to stridulation variation. Nonetheless, we ignore how low-frequency sounds impact freshwater ecosystems, and the intricate relationship between soundscapes and aquatic life in urban settings. Implications for insect conservation: There is a correlation between anthropophony and variations in the stridulation of aquatic bugs. This highlights the possibility of sound pollution on freshwater systems, emphasizing the need for assessment to better understand and prevent potential harm to aquatic insects.