bluuebunny/crossref_metadata_2025_split huggingface dataset:
Invasive weed disrupts facilitation of nutrient uptake in grass-clover assemblage
Authors: Zhang Wei , Muir Rick , Dickinson Nicholas 
Published: January 2024

Abstract

Nutrient constraints in low-fertility soil were modified by different species combinations.

Grass-clover assemblages benefited both species in terms of nutrient procurement.

Interplay of competition and facilitation is demonstrated.

An invasive weed removed essential nutrients from the grazing cycle.

To investigate the interplay of competition and facilitation between plants in low-fertility pasture grasslands of New Zealand, we compared nutrient uptake and acquisition of key nutrients of three species from different functional groups. Combinations of Pilosella officinarum (mouse-eared hawk-weed, an invasive weed), Trifolium repens (white clover, a nitrogen fixer) and Dactylis glomerata (cocksfoot, a pasture grass) were planted into a soil with low-to-deficient concentrations of key nutrients. Highest yields were achieved by the grass growing alone but, when the clover and grass had grown together, there were complementary benefits in terms of procurement of a wide range of nutrients from soil despite lower root biomass. The invasive weed negated these benefits, and soil nutrients were exploited less efficiently when Pilosella had grown alone or in a mixture with the other species. Competition from the weed removed the benefits of grass-legume coexistence. These findings are interpreted to suggest that requirements for legumes to be the main source of nitrogen in pasture grasslands may be compromised unless competitive weeds are controlled to avoid disrupted procurement of key nutrients. It is likely these constraints to nutrient procurement would similarly impact conservation grasslands.
Lymph node evaluation and surgical procedure selection for non-small cell lung cancer
Authors: Zhaoming Gao , Zhenfa Zhang 
Published: January 2024

AbstractLymph node metastasis is a common mode of metastasis in non-small cell lung cancer (NSCLC). Correct lymph node staging is crucial to the selection of treatment and the assessment of the prognosis of patients, and the selection of appropriate lymph node resection can prolong the survival of patients and reduce surgical trauma.In this review, the preoperative lymph node evaluation methods and the common intraoperative lymph node resection methods of NSCLC are reviewed, and the advantages and disadvantages of each method are compared. The purpose of this review is to summarize the latest research progress in the evaluation and resection of NSCLC lymph nodes, so as to select appropriate evaluation and resection methods in clinical work.
Resident dictation of operative notes: a reimbursement comparison and mixed-methods analysis
Authors: Wang Theresa N. , Chen J. C. , Weirich David E. , Kalady Matthew F. , Pawlik Timothy M. , Huang Emily 
Published: January 2024

Abstract
Purpose
Surgical documentation is crucial to ensure quality patient care and accurate coding and billing. Operative dictation also serves as a valuable educational opportunity for surgical trainees. However, resident dictations may not fully capture procedural details and complexities, resulting in missed revenue opportunities. On July 1, 2021, our university-based surgery department implemented a policy requiring attendings to dictate all operative reports. The purpose of this mixed-method study was to investigate the financial impact of this policy and explore differences in resident and attending dictations.

Methods
Core general surgery operations performed by the Department of Surgery between July 1, 2020 and June 30, 2022 were identified from billing data. The surgeon, current procedural terminology (CPT) and modifier codes, and relative value units (RVUs) for each case were acquired. Surgeons not present for the entire study period or cases requiring multiple surgeons were excluded. Descriptive statistics and Kolmogorov–Smirnov (KS) non-parametric tests compared pre- and post-policy RVU distributions on overall charges and 18 key general surgery operations. Targeted thematic analysis was performed on operative reports pre- and post-policy to explore resident and attending differences.

Results
A total of 42 attendings performed 16,233 cases, billing 28,560 CPT codes (50.3% pre- vs. 49.7% post-policy). There was a small but statistically significant increase in RVU distribution post-policy, mean 20.2 pre- vs. 20.3 RVUs post-, $4372 pre- vs. $4418 per case post-, KS = 0.02 (p = 0.009). Specifically, higher RVU distributions were seen among attending-dictated cases for melanoma (p = 0.009), minimally invasive ventral hernia repair (VHR, p = 0.008), parathyroidectomy (p < 0.001), anorectal incision and drainage (p = 0.003) and anorectal exam under anesthesia (p = 0.029). Higher RVU distributions were noted among resident-dictated, attending-edited cases for partial colectomy (p = 0.043), and open VHR (p = 0.004). No differences were noted among the remaining operations (p > 0.05). Three major themes were noted from focused sampling of 112 operative reports: billable items, clinical/surgical reasoning, and technical details. Differences in billable items and clinical and surgical reasoning were the most influential on modifying clinical implications of operative notes. Themes and differences were consistent regardless of surgeon or specialty.

Conclusion
Adopting an attending-only operative dictation policy yielded a small increase in billable RVUs, predominantly from select operations. Gaps in coding-directed language and depth of clinical reasoning were noted in resident dictations. These findings reveal an educational opportunity that concomitantly optimizes patient care, resident education, and procedural revenue.
Self-Adaptive Forensic-Based Investigation Algorithm with Dynamic Population for Solving Constraint Optimization Problems
Authors: Cai Pengxing , Zhang Yu , Jin Ting , Todo Yuki , Gao Shangce 
Published: January 2024

AbstractThe Forensic-Based Investigation (FBI) algorithm is a novel metaheuristic algorithm. Many researches have shown that FBI is a promising algorithm due to two specific population types. However, there is no sufficient information exchange between these two population types in the original FBI algorithm. Therefore, FBI suffers from many problems. This paper incorporates a novel self-adaptive population control strategy into FBI algorithm to adjust parameters based on the fitness transformation from the previous iteration, named SaFBI. In addition to the self-adaptive mechanism, our proposed SaFBI refers to a novel updating operator to further improve the robustness and effectiveness of the algorithm. To prove the availability of the proposed algorithm, we select 51 CEC benchmark functions and two well-known engineering problems to verify the performance of SaFBI. Experimental and statistical results manifest that the proposed SaFBI algorithm performs superiorly compared to some state-of-the-art algorithms.
Hydrochars as slow-release phosphorus fertilizers for enhancing corn and soybean growth in an agricultural soil
Authors: Khosravi Anahita , Yuan Yanfei , Liu Qiang , Zheng Hao , Hashemi Masoud , Tang Yuanzhi , Xing Baoshan 
Published: January 2024

AbstractHydrochars as carbonaceous amendments rich in porous structures and nutrients can be used as promising slow-release fertilizers and soil health amendments. Yet, the performance of hydrochars derived from different feedstocks in improving crop plant growth and soil phosphorus (P) availability is not well understood. Here, a batch of sewage sludge (SS) and chicken manure (CM) derived hydrochars (SSCs and CMCs) were produced at 125 and 225 °C (SS125/225 and CM125/225) to investigate their characteristics and performances in improving the seedling growth of two representative crops, soybean (Glycine max) and corn (Zea mays), as well as the P availability in an acidic agricultural soil in comparison with triple super phosphate (TSP), a conventional P fertilizer. Compared with CMCs, SSCs with more porous and rougher surfaces contained relatively lower contents of carbon (C; 32.3–33.3%), hydrogen (H; 3.9–4.69%), and potassium (K; 29.2–51 mg g−1), but higher contents of P (37.4–85.4%) and nitrogen (N; 2.26–4.9%), as well as more O-containing and N-containing functional groups. The impacts of hydrochars on soybean and corn growth showed distinct variations. The application of SSCs showed little effect on soybean growth (i.e., biomass, chlorophyl contents, and number of leaves), while CMCs significantly increased its total dry biomass by 23.2–66.2%. For corn, both SSCs and CMCs increased the total dry biomass by 32.8–92.4% and 21.8–69.7%, respectively, compared to those in the un-amended soils. In addition, compared with the higher temperature hydrochars (SS225 and CM225), the lower temperature ones (SS125 and CM125) increased the total dry biomass by 24.6% and 34.9% for soybean and 44.8% and 39.3% for corn, respectively. The significant improvement in crop growth by hydrochars was mainly due to the direct nutrient supply (particularly P) by the hydrochars, which was supported by the increased soil and shoot P concentrations. Moreover, hydrochar application led to a rise in soil water soluble P (WSP) levels. However, as time progressed, these levels fell due to the fixation and adsorption of P via precipitation, electrostatic attraction, and ion exchange on the hydrochars. Contrarily, TSP maintained persistently high soil WSP levels, increasing the leaching risk of P through the soil profile. Our results provide direct evidence for hydrochars as slow-release P fertilizers to enhance crop growth and production and give better insights for producing functionalized P-rich chars as an alternative to chemical P fertilizers to maintain sustainable agricultural production.
Graphical Abstract
Late-onset vitamin K deficiency bleeding: a preventable yet prevailing cause of intracranial hemorrhage in young infants—an ambispective descriptive study
Authors: Annadurai Aswini , Delhi Kumar C. G. , S. M. Praveen , Parameswaran Narayanan 
Published: January 2024

Abstract
Background
Vitamin K deficiency bleeding (VKDB) may present as intracranial hemorrhage (ICH) in young infants, which results in severe morbidity and mortality. This study aims to determine the clinical presentation, risk factors, and outcome of children with VKDB.

Methods
This was an ambispective descriptive study conducted from January 2015 to August 2023 in southern India. Infants from 8 days to 6 months of age, diagnosed as VKDB based on bleeding with elevated PIVKA (protein induced by vitamin K absence) and prolonged prothrombin time, which is rapidly corrected 24 h after administration of vitamin K, were included. Infants with bleeding due to other causes, such as inherited clotting factor deficiency, cholestasis, and sepsis, were excluded. A structured data collection proforma was used to collect data.

Results
Out of 62 cases, 28 were excluded due to various causes, and 34 were analyzed. The median age at symptom onset was 54 days, and males were 22 (64.7%). Term gestation was 25 (73.5%), and low birth weight was 13 (38.2%), and 8 (23.5%) required NICU admission. All received vitamin K prophylaxis at birth. Thirty-three (97%) were exclusively breastfed. The most common presenting complaints were poor activity and convulsion. A total of 97% of children had ICH, and 5 (14.7%) had skin bleed. Twenty-three (67.6%) infants had complete recovery, 6 (17.6%) had sequelae (2 had hemiparesis, 2 monoparesis, and 2 quadriplegia), and 5 (14.7%) children expired.

Conclusion
Vitamin K deficiency commonly presents as intracranial hemorrhage in infants. Exclusive breastfeeding is a significant risk factor for vitamin K deficiency, followed by low birth weight and prematurity.
Lithospheric Rheology and Crustal Deformation Across the Northeastern Tibet and Their Implications for Plateau Growth
Authors: Li Zhangjun , Cheng Feng , Li Yujiang , Liu Lei , Zhu Liangyu , Wang Yang , Hao Ming , Zhuang Wenquan , Xiong Renwei , Gan Weijun 
Published: January 2024

AbstractUnderstanding lithospheric rheology is crucial in investigating tectonic evolution of intra‐continental tectonic boundary. Here, we use geodetic observations to infer lithospheric rheology across the northeastern Tibet based on a 2D viscoelastic model. Our findings reveal a lower‐crust viscosity of <1022 Pa·s underneath its margins, lower than those estimated underneath its vicinities. By comparing deformation patterns and lithospheric rheology here with those observed in the eastern Tibet, we propose that lateral variations in lower‐crust viscosity control deformation patterns and topographic gradients along the Tibet margins. The presence of low viscosity lower‐crust can lead to the development of contrasting topographic gradients and shape the plateau's geomorphology and deformation characteristics during outward growth of the Tibet. We here emphasize the subtle variations in the lower‐crust rheology between deforming blocks and the corresponding mountain ranges, which play an important role in orogeny along the intracontinental convergence boundary.
Reproducible stability of verbal and spatial functions along the menstrual cycle
Authors: Pletzer Belinda , Bodenbach Hannah , Hoehn Marcel , Hajdari Linda , Hausinger Tobias , Noachtar Isabel , Beltz Adriene M. 
Published: January 2024

AbstractRecent studies have reported brain changes in response to ovarian hormonal fluctuations along the menstrual cycle. However, it remains unclear, whether these brain changes are of an adaptive nature or whether they are linked to changes in behavior along the menstrual cycle, particularly with respect to cognitive performance. To address this knowledge gap, we report results from 3 well-powered behavioral studies with different task designs, leveraging the advantages of each design type. In all three studies we assessed whether verbal or spatial performance (i) differed between cycle phases, (ii) were related to estradiol and / or progesterone levels and (iii) were moderated by individual hormone sensitivity as estimated by premenstrual symptoms. Overall, results of all three studies point towards a null effect of menstrual cycle phase and – to a lesser extent – ovarian hormones on verbal and spatial performance and provided no evidence for a moderation of this effect by individual hormone sensitivity. We conclude that there is substantial consistency in verbal and spatial performance across the menstrual cycle, and that future studies of intra-individual variation are needed.
Metabolic heterogeneity affects trastuzumab response and survival in HER2-positive advanced gastric cancer
Authors: Wang Jun , Sun Na , Kunzke Thomas , Shen Jian , Feuchtinger Annette , Wang Qian , Meixner Raphael , Gleut Ronan Le , Haffner Ivonne , Luber Birgit , Lordick Florian , Walch Axel 
Published: January 2024

Abstract
Background
Trastuzumab is the only first-line treatment targeted against the human epidermal growth factor receptor 2 (HER2) approved for patients with HER2-positive advanced gastric cancer. The impact of metabolic heterogeneity on trastuzumab treatment efficacy remains unclear.

Methods
Spatial metabolomics via high mass resolution imaging mass spectrometry was performed in pretherapeutic biopsies of patients with HER2-positive advanced gastric cancer in a prospective multicentre observational study. The mass spectra, representing the metabolic heterogeneity within tumour areas, were grouped by K-means clustering algorithm. Simpson’s diversity index was applied to compare the metabolic heterogeneity level of individual patients.

Results
Clustering analysis revealed metabolic heterogeneity in HER2-positive gastric cancer patients and uncovered nine tumour subpopulations. High metabolic heterogeneity was shown as a factor indicating sensitivity to trastuzumab (p = 0.008) and favourable prognosis at trend level. Two of the nine tumour subpopulations associated with favourable prognosis and trastuzumab sensitivity, and one subpopulation associated with poor prognosis and trastuzumab resistance.

Conclusions
This work revealed that tumour metabolic heterogeneity associated with prognosis and trastuzumab response based on tissue metabolomics of HER2-positive gastric cancer. Tumour metabolic subpopulations may provide an association with trastuzumab therapy efficacy.

Clinical trial registration
The patient cohort was conducted from a multicentre observational study (VARIANZ;NCT02305043).
Collagen I-induced VCAN/ERK signaling and PARP1/ZEB1-mediated metastasis facilitate OSBPL2 defect to promote colorectal cancer progression
Authors: Lin Kang , Zhao Yun , Tang Yuqi , Chen Ying , Lin Moubin , He Luwei 
Published: January 2024

AbstractThe global burden of colorectal cancer (CRC) has rapidly increased in recent years. Dysregulated cholesterol homeostasis facilitated by extracellular matrix (ECM) remodeling transforms the tumor microenvironment. Collagen I, a major with ECM component is highly expressed in colorectal tumors with infiltrative growth. Although oxysterol binding protein (OSBP)-related proteins accommodate tumorigenesis, OSBPL2, which is usually involved in deafness, is not associated with CRC progression. Therefore, we aimed to investigate the pathological function of OSBPL2 and identify the molecular link between ECM-Collagen I and OSBPL2 in CRC to facilitate the development of new treatments for CRC. OSBPL2 predicted a favorable prognosis in stage IV CRC and substantially repressed Collagen I-induced focal adhesion, migration, and invasion. The reduction of OSBPL2 activated ERK signaling through the VCAN/AREG/EREG axis during CRC growth, while relying on PARP1 via ZEB1 in CRC metastasis. OSBPL2 defect supported colorectal tumor growth and metastasis, which were suppressed by the ERK and PARP1 inhibitors SCH772984 and AG14361, respectively. Overall, our findings revealed that the Collagen I-induced loss of OSBPL2 aggravates CRC progression through VCAN-mediated ERK signaling and the PARP1/ZEB1 axis. This demonstrates that SCH772984 and AG14361 are reciprocally connective therapies for OSBPL2Low CRC, which could contribute to further development of targeted CRC treatment.
HECTD3 inhibits NLRP3 inflammasome assembly and activation by blocking NLRP3-NEK7 interaction
Authors: Cheng Zhuo , Huang Maobo , Li Wei , Hou Lei , Jin Li , Fan Qijin , Zhang Linqiang , Li Chengbin , Zeng Li , Yang Chuanyu , Liang Bin , Li Fubing , Chen Ceshi 
Published: January 2024

AbstractThe NLRP3 inflammasome plays an important role in protecting the host from infection and aseptic inflammation, and its regulatory mechanism is not completely understood. Dysregulation of NLRP3 can cause diverse inflammatory diseases. HECTD3 is a E3 ubiquitin ligase of the HECT family that has been reported to participate in autoimmune and infectious diseases. However, the relationship between HECTD3 and the NLRP3 inflammasome has not been well studied. Herein, we show that HECTD3 blocks the interaction between NEK7 and NLRP3 to inhibit NLRP3 inflammasome assembly and activation. In BMDMs, Hectd3 deficiency promotes the assembly and activation of NLRP3 inflammasome and the secretion of IL-1β, while the overexpression of HECTD3 inhibits these processes. Unexpectedly, HECTD3 functions in an E3 activity independent manner. Mechanically, the DOC domain of HECTD3 interacts with NACHT/LRR domain of NLRP3, which blocks NLRP3-NEK7 interaction and NLRP3 oligomerization. Furthermore, HECTD3 inhibits monosodium urate crystals (MSU)-induced gouty arthritis, a NLRP3-related disease. Thus, we reveal a novel regulatory mechanism of NLRP3 by HECTD3 and suggest HECTD3 could be a potential therapeutic target for NLRP3-dependent pathologies.
Atomic-scale manipulation of polar domain boundaries in monolayer ferroelectric In2Se3
Authors: Zhang Fan , Wang Zhe , Liu Lixuan , Nie Anmin , Li Yanxing , Gong Yongji , Zhu Wenguang , Tao Chenggang 
Published: January 2024

AbstractDomain boundaries have been intensively investigated in bulk ferroelectric materials and two-dimensional materials. Many methods such as electrical, mechanical and optical approaches have been utilized to probe and manipulate domain boundaries. So far most research focuses on the initial and final states of domain boundaries before and after manipulation, while the microscopic understanding of the evolution of domain boundaries remains elusive. In this paper, we report controllable manipulation of the domain boundaries in two-dimensional ferroelectric In2Se3 with atomic precision using scanning tunneling microscopy. We show that the movements of the domain boundaries can be driven by the electric field from a scanning tunneling microscope tip and proceed by the collective shifting of atoms at the domain boundaries. Our density functional theory calculations reveal the energy path and evolution of the domain boundary movement. The results provide deep insight into domain boundaries in two-dimensional ferroelectric materials and will inspire inventive applications of these materials.
Lsr2 acts as a cyclic di-GMP receptor that promotes keto-mycolic acid synthesis and biofilm formation in mycobacteria
Authors: Ling Xiaocui , Liu Xiao , Wang Kun , Guo Minhao , Ou Yanzhe , Li Danting , Xiang Yulin , Zheng Jiachen , Hu Lihua , Zhang Hongyun , Li Weihui 
Published: January 2024

AbstractCyclic di-GMP (c-di-GMP) is a second messenger that promotes biofilm formation in several bacterial species, but the mechanisms are often unclear. Here, we report that c-di-GMP promotes biofilm formation in mycobacteria in a manner dependent on the nucleoid-associated protein Lsr2. We show that c-di-GMP specifically binds to Lsr2 at a ratio of 1:1. Lsr2 upregulates the expression of HadD, a (3R)-hydroxyacyl-ACP dehydratase, thus promoting the synthesis of keto-mycolic acid and biofilm formation. Thus, Lsr2 acts as a c-di-GMP receptor that links the second messenger’s function to lipid synthesis and biofilm formation in mycobacteria.
Attitudes and barriers towards deprescribing in older patients experiencing polypharmacy: a narrative review
Authors: Robinson Michael , Mokrzecki Sophie , Mallett Andrew J. 
Published: January 2024

AbstractPolypharmacy, commonly defined as ≥5 medications, is a rising public health concern due to its many risks of harm. One commonly recommended strategy to address polypharmacy is medication reviews, with subsequent deprescription of inappropriate medications. In this review, we explore the intersection of older age, polypharmacy, and deprescribing in a contemporary context by appraising the published literature (2012–2022) to identify articles that included new primary data on deprescribing medications in patients aged ≥65 years currently taking ≥5 medications. We found 31 articles were found which describe the current perceptions of clinicians towards deprescribing, the identified barriers, key enabling factors, and future directions in approaching deprescribing. Currently, clinicians believe that deprescribing is a complex process, and despite the majority of clinicians reporting feeling comfortable in deprescribing, fewer engage with this process regularly. Common barriers cited include a lack of knowledge and training around the deprescribing process, a lack of time, a breakdown in communication, perceived ‘abandonment of care’, fear of adverse consequences, and resistance from patients and/or their carers. Common enabling factors of deprescribing include recognition of key opportunities to instigate this process, regular medication reviews, improving lines of communication, education of both patients and clinicians and a multidisciplinary approach towards patient care. Addressing polypharmacy requires a nuanced approach in a generally complex group of patients. Key strategies to reducing the risks of polypharmacy include education of patients and clinicians, in addition to improving communication between healthcare providers in a multidisciplinary approach.