bluuebunny/crossref_metadata_2025_split huggingface dataset:
Videostrobokymography in the diagnosis of laryngeal diseases
Authors: Gotovyakhina T. V. , Stepanova Yu. E. 
Published: January 2025

Introduction. Videolaryngoscopy and videolaryngostroboscopy are the “gold standard” for the diagnosis of laryngeal diseases. During a videolaryngoscopy, an anatomical and functional assessment of the state of the larynx is performed. Videolaryngostroboscopy visualizes the vibratory cycle of the vocal folds in order to provide a more detailed functional assessment. Videostrobokymography allows to perform a qualitative and quantitative analysis of the vibratory cycle.Aim. To evaluate the indicators of videostrobokymography in functional and organic dysphonia.Materials and methods. 9 patients were examined. In one case, there was no pathology of the larynx. Among the functional disorders, two cases of hypofunctional and one of hyperfunctional dysphonia are presented. Organic diseases: chronic catarrhal laryngitis, Reinke’s edema, vocal fold nodules, vocal fold polyp, vocal fold injury after phonosurgery. Videolaryngoscopy, videolaryngostroboscopy, and videostrobokymography were performed. The strobokimograms were evaluated qualitatively by the presence of periodic oscillations of the vocal folds, phase and amplitude symmetry, mucous wave, the shape of lateral and medial peaks, and the shape of the gap in the opening phase. For the quantitative analysis, the opening quotient (OQ) was determined, which objectively reflected the duration of the opening phase.Results. Normally, phase and amplitude symmetry were preserved, the mucous wave was visualized, lateral and medial peaks of vibrations were pronounced, the gap in the opening phase was in the shape of a rhomb, OQ was 0.5. Pathological changes included: a decrease in the amplitude of vibrations, an increase or decrease in the mucous wave, phase and amplitude asymmetry, a change in the shape of lateral and medial peaks, a change in the shape of the gap in the opening phase. The OQ index varied from 0.42 to 1 depending on the duration of the opening phase in various diseases of the larynx.Conclusions. Videostrobokymography makes it possible to assess the indicators of the vibratory cycle in more detail, conduct a graphical analysis, compare the picture at different levels of the glottis, and obtain objective data. The method can be used in the in-depth diagnosis and evaluation of the results of treatment of diseases of the larynx in addition to videolaryngostroboscopy.
Forsythiaside A Ameliorates Inflammation by Regulating the Autophagy in Methotrexate-induced Intestinal Mucositis
Authors: Lang Wuying , Zhang Jiayi , Xiao Xuejun , Cheng Min , Zheng Xin , Gong Haizhou , Ali Ihsan , Zhao Yongping , Jia Feng , Wang Zhe , Wang Jing , Li Wei , Zhang Haihua 
Published: January 2025

Background:
Methotrexate (MTX) effectively eliminates cancerous cells but can also
cause inflammation intestinal, known as mucositis. Forsythiaside A (FTA) from Forsythia suspensa
has shown promise in relieving mucositis by targeting the NLRP3 pathways. Since NLRP3
inflammasome activation is negatively regulated by autophagy, this study explores how FTAmediated
autophagy affects NLRP3 inflammasome in treating MTX-induced intestinal inflammation.

Method:
Intestinal mucositis was induced in rats with MTX. FTA's impact was assessed using
HE staining and ELISA. The mechanism was studied using immunofluorescence, western blot,
and ELISA.

result:
FTA treatment resulted in reduced levels of D-lactic acid and diamine oxidase (DAO) in
MTX-treated rats. Western blot and immunofluorescence analyses revealed up-regulation of Beclin-
1 and LC3II/I, accumulation of LC3, and down-regulation of p62 expression levels in MTXtreated
rats following 40 or 80 mg/kg FTA intervention. However, when the autophagy inhibitor
3-MA was used, the intestinal pathology was exacerbated, the inflammatory scores increased,
and serum levels of TNF-α, IL-1β, and IL-18 were elevated. Western blotting indicated decreased
LC3II/I expression, while NLRP3, cleaved caspase 1, and cleaved IL-1β expressions
were upregulated.

Conclusion:
These findings suggested that FTA alleviated MTX-treated intestinal mucositis by
activating autophagy, which in turn inhibits the NLRP3 inflammasome.
Late Endovascular Treatment for Ischemic Stroke with Moderate to
Large Infarct Volume is Associated with a better Clinical Prognosis
Authors: Jiang Peng , Zhang Sheng , Yu Weitao , Shi Zongjie , Jiang Xinzhao , Wang Xu , Lin Longting , Parsons Mark , Guo Wenting 
Published: January 2025

Objective:
The concept of “time is brain” is crucial for the reperfusion therapy of ischemic
stroke. However, the Infarct Growth Rate (IGR) varies among individuals, which is regarded
as a more powerful factor than the time when determining infarct volume and its association
with clinical outcomes. For stroke patients with a similar infarct volume, a longer time from
stroke Onset to Imaging (OTI) correlates with a lower IGR, which may indicate a better prognosis.
This study aimed to compare the prognoses of patients with anterior circulation stroke who received
Endovascular Treatment (EVT), specifically comparing early EVT vs. late EVT.

Methods:
We analyzed 255 patients with acute anterior circulation stroke due to large vessel occlusion
and who have successfully undergone recanalization after EVT. All patients were divided
into the late (OTI≥6 hours) and early (<6 hours) time window groups and compared. The primary
outcome was moderate functional prognosis, defined as a modified Rankin Scale (mRS) ≤3 at 90
days. The secondary outcome was No Significant Infarct Expansion (NSIE), defined as a reduction
of less than 2 points on the Alberta Stroke Program Early CT Score (ASPECTS).

Results:
In the moderate to large infarct subgroup, the late time window EVT was independently
associated with a higher rate of moderate functional outcome (P =0.007) and NSIE (P =0.001);
mediation analysis showed that NSIE partially mediated the effects of the late time window EVT
on moderate functional outcome (coefficient: 0.112, 95% CI: 0.051 to 0.239, P =0.011); however,
these associations were not consistent in the small infarct group.

Conclusion:
For anterior circulation stroke patients who received EVT according to current guidelines,
those with moderate to large infarct volume and having a longer OTI had better clinical outcomes
than those who had a shorter OTI and were more suitable for EVT.
Galegine, a Bioprivileged Alkaloid from Tithonia tubaeformis: Antipyretic Activity Insights
Authors: Nawaz Noor Ul Ain , Saeed Muhammad , Faizi Shaheen , Ali Irfan , Shafiq Muhammad , Shahid Muhammad , Rabbi Fazle , Khan Fahim Ullah , Sharif Rabeea , Ul-Haq Zaheer , Khan Khalid Mohammed , Hemeg Hassan A , Al Abdulmonem Waleed , Aljohani Abdullah S. M , Rauf Abdur 
Published: January 2025

Background:
In continuation of our chemical and biological work on Tithonia tubaeformis,
we evaluated the antipyretic activity of its extract which on fractionation gives a pure alkaloid
galegine. Galegine a bioprivileged compound, is a hemiterpene bearing a guanidine group,
which holds significant importance in medicinal chemistry. Biological activities such as antimicrobial,
antidiabetic, anti-inflammatory, cardiovascular, anticancer, and antihypertensive, are often
associated with guanidine-containing molecules.

Objective:
Given the biological importance of guanidine and in search of safe antipyretic agents
from natural resources, an in vivo antipyretic activity of methanolic extract of T. tubaeformis and
galegine was conducted to discover a potential hypothermic drug candidate from the plant.

Methods:
In vivo, the antipyretic activity of galegine (5, 25, and 50 mg/kg doses) and methanolic
extract of T. tubaeformis (50, 100, and 200 mg/kg doses) was investigated by employing yeastinduced
pyrexia in mice model. In silico molecular docking analysis involving target enzymes cyclooxygenase-
1 (COX-1), cyclooxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1
(mPGES-1) was conducted. Additionally, galegine underwent ADME/T profiling using SwissADME
and Protox-II tools to evaluate its bioavailability and safety profiles.

Results:
Both the extract and galegine showed a progressive dose-dependent reduction in body
temperatures of the hyperthermic test animals. Moreover, in silico molecular docking analysis revealed
significant binding affinities ranging from -3.58 to -5.41 kcal/mol. ADME/T analyses of
galegine predicted its high drug-likeness and good safety profile.

Conclusion:
These biological and computational approaches supported T. tubaeformis in addressing
pyrexia, with the isolated compound galegine emerging as a promising antipyretic agent.
High-value Utilization of Liubao Tea Residues: Physicochemical Properties and Immunomodulatory Activity of Alkali-extracted Cell Wall
Polysaccharides
Authors: Li Beibei , Sun Yizheng , Wang Huiqin , Ji Xinying 
Published: January 2025

Objective:
Liubao tea residues, often discarded as waste, may contain valuable bioactive
compounds as polysaccharides. To characterize the physicochemical properties of polysaccharides
extracted by 4 mol/L KOH (KTP) and investigate its effects on macrophage activation and immune
response.

Method:
KTP was extracted using an alkaline method. Physicochemical characterization was performed
using monosaccharide analysis, molecular weight assessment, FT-IR spectroscopy, XRD,
and NMR spectroscopy. Immunomodulatory effects were evaluated through macrophage activation
assays, focusing on NO production, cytokine release, and NF-κB pathway modulation.

Results:
Monosaccharide analysis identified KTP as a composite of arabinose, mannose, galactose,
glucose, and xylose with distinct variations in abundance. Molecular weight analysis revealed KTP
as a heterogeneous polysaccharide with fractions KTP-1 and KTP-2 of different molecular sizes.
Structural characterization analysis showed specific functional groups, bond arrangements, and helical
conformations, elucidating KTP’s intricate surface morphologies and semi-crystalline nature.
Additionally, immunomodulatory studies demonstrated KTP’s activation of macrophage pathways
via the NF-κB pathway, increasing nitric oxide (NO) and pro-inflammatory cytokine production
dose-dependently.

Conclusion:
This study reveals KTP's rich structural diversity and potent immunomodulatory activity,
highlighting its potential as a natural immune booster and possible application in developing
functional foods.
Characterization of Lithium-containing Fly Ash Derived from Coal-fired Power Plants in Guizhou Province, China
Authors: Mu Xiwei , Lv Longjiao , Cao Xiru , Liu Jingfeng , Li Fayue , Liu Chunying , Ren Yuting , Ban Cao , Ren Bozhi , Xu Shengjiao , Wu Zhengchuan , Zhu Guocheng 
Published: January 2025

Background:
Coal-fired power plants, such as those located in Guizhou, are generating
substantial amounts of fly ash. This fly ash is rich in lithium, a highly valuable metal utilized in numerous
industrial applications. Effective extraction of these metals necessitates a thorough understanding
of their characteristics within the fly ash.

Methods:
Multiple analytical techniques were used to study fly ash's physicochemical properties.
Meanwhile, wet magnetic separation and acid-base methods are employed to separate its mineral
phases.

Results:
The fly ash mainly contains mullite, glass, and iron microspheres, showing spherical particle
morphology. It's rich in O, Si, Al, Fe, C, and oxides like SiO2, Al2O3, Fe2O3. Most particles are
smaller than 20 μm. Lithium in the ash existed as Li-O-M compounds, mainly in the non-magnetic
and glass phases.

Conclusion:
Overall, these findings have deepened our understanding of fly ash and the distribution
of lithium within it, providing a foundation for the recovery of lithium and other valuable elements.
From Paper to Digital: Evaluating Electronic Medical Records and their
Compliance with EMA Guidelines in European Clinical Trials
Authors: Stabile Stefano , Testoni Sara , Franchina Veronica , Betti Marta , Mannozzi Francesca , Ferrari Alessandra , Federici Irene , Doungue Ludovic , Cagnazzo Celeste 
Published: January 2025

Background:
Over the past decade, there has been a significant shift from paper-based
to digital medical record management, driven largely by advances in digital technology. This transition
has led to widespread adoption of Electronic Medical Records (EMRs), with the expectation
that paper documentation will soon be fully replaced. In response, the European Medicines Agency's
“Guideline on Computerised Systems in Clinical Trials” outlines essential criteria for validated
EMR systems to ensure data integrity and security, and sets standards for electronic source documents
in clinical trials.

Methods:
From December 2023 to March 2024, the Italian Group of Data Managers and Clinical
Research Coordinators (GIDMcrc) conducted an online survey across clinical research sites in Italy,
France and Belgium to assess the characteristics of medical records and source documents.

Results:
The survey was completed by 37 centres: 70.3% from Italy, 16.2% from France and
13.5% from Belgium. Most sites use a mixed paper/electronic Source Document (SD) system
(72.3%), with fewer centres having fully electronic SD systems (13.5%) or fully paper-based systems
(16.2%). EMR systems are used in 70.3% of sites, but only 23.8% comply with EMA guidelines
for computerised systems. A country-specific analysis was also conducted to further explore
the situations in Italy and France/Belgium.

Conclusion:
Despite the widespread use of electronic medical records (EMRs) in Italy, France
and Belgium, Italy lags behind the other two countries in terms of digitization. Despite the presence
of an EMR, many centres still use a mixed system of paper and electronic source documents.
There is also a lack of awareness regarding EMA and GCP standards, particularly concerning
training and system testing. The higher response rate from Italian centres highlights the need for a
larger sample in France and Belgium, and a follow-up survey would be beneficial for assessing
progress and refining corrective actions.
Sesquiterpene Coumarins as Promising Antiviral Agents: An In-Silico Study
Authors: Dastan Dara , Ebadi Ahmad 
Published: January 2025

Background:
Sesquiterpene coumarins are a unique class of natural compounds with a wide range of biological activities. These C15 terpenes are connected by ether or carbon-carbon bonds to coumarin derivatives. Sesquiterpene coumarins that include a 7-hydroxylcoumarin (umbelliferone) moiety have significant antiviral properties. The natural flexibility of these compounds reduces the likelihood of developing resistance, which is often seen in viruses due to high mutation rates.

Objective:
The lessons learned from the coronavirus pandemic experience emphasize the importance of preparedness for future viral outbreaks in the medical community. Conse-quently, fast and reliable assessment methods, such as in silico techniques, are crucial in drug discovery.

Methods:
In this study, we used in silico studies to evaluate the potential antiviral effects of various sesquiterpene coumarins.

Results:
The binding free energy to the spike protein of SARS-CoV-2 suggested that 5′-hydroxyumbelliprenin (2), conferol (9), 8′-acetoxy-5′-hydroxyumbelliprenin (3), and Sanandajine (1) could be promising antiviral candidates.

Conclusion:
These compounds have unique physicochemical characteristics and occupy distinct chemical spaces compared to synthetic libraries; therefore, the criteria for drug-likeness need to be adjusted for this series of compounds.
Association of Angiotensin-converting Enzyme 2 (ACE2) Gene Polymorphisms with the Risk of COVID-19 Severity in the Malaysian Population
Authors: Chong Eric Tzyy Jiann , Bernard Bronsson Khusaidie , Lee Heng Gee , Tan Yee Ann , Chan Lan Lan , Lee Qin Zhi , Saw Yen Tsen , Wong Yiko , Aizudeen Bin Zakaria Ahmad , Lee Ping-Chin 
Published: January 2025

Introduction:
The global impact of coronavirus disease 19 (COVID-19), caused by the
SARS-CoV-2, has presented unprecedented challenges worldwide. Previous studies have shown an
association between angiotensin-converting enzyme 2 (ACE2) single nucleotide polymorphisms
(SNPs) and the risk of COVID-19 severity in different populations. However, it remains unclear in
the Malaysian population.

Method:
Therefore, this study aims to investigate the association between four ACE2 SNPs
(rs2074192, rs2285666, rs714205, and rs4240157) and COVID-19 severity in the Malaysian population.
In brief, genomic DNA was extracted from the peripheral blood of 215 recovered COVID-19
patients. The ACE2 SNPs were genotyped using a real-time polymerase chain reaction approach.
Odds ratios and 95% confidence intervals were calculated using the MedCalc software for risk association
analysis. The linkage disequilibrium (LD) and haplotype analyses were assessed using the
SHEsis software.

Result:
This study revealed that the presence of the recessive T allele in the rs2074192 SNP increased
the risk of COVID-19 severity in the Malaysian population. On the other hand, the recessive
T allele in the rs2285666 SNP and the recessive G allele in the rs714205 SNP are reduced risk factors
for COVID-19 severity in the population. LD analysis showed that these four SNPs are independent
of each other. For haplotype analysis, the CCGT and CTGC haplotypes exhibited a decreased
risk, while the TCCC haplotype revealed an elevated risk of severe COVID-19 in the Malaysian
population.

Conclusion:
This study suggests a significant association between the independent ACE2 SNPs
(rs2074192, rs2285666, and rs714205) and haplotypes with COVID-19 severity in the Malaysian
population. This finding can assist in determining an individual’s susceptibility to experiencing severe
COVID-19 and in predicting their response to drugs.
SARS-CoV-2 Infections and Long COVID as Drivers of Accelerated Biological Aging in Endothelial Cells Related to Oxidative Stress
Authors: Tricarico Gerardo , Travagli Valter 
Published: January 2025

Abstract:
Recent studies have reported that severe cases of COVID-19 are primarily characterized
by endotheliitis (or endothelialitis), whose severity is related to the pre-existing or current damage to
the complex structure that lines the surface of the vascular endothelium, most notably the glycocalyx.
Such damage results in the loss of many endothelial cell functions. To restore these functions, the
cell must repair the glycocalyx that covers it. However, this is not always possible, especially when
the repair capacity of such an exo-endo-cytoplasmic cell structure is limited due to cellular senescence.
If the gene repair system remains activated, the damaged glycocalyx can be reconstituted via
specific transcriptional pathways in response to oxidative and reductive stresses. Despite the experimental
evidence in favour of this hypothesis, many of the recently published reviewrs on long
COVID do not mention the use of therapeutic treatments via specific 'oxidative eustress'. In fact,
mild and controlled oxidative stress is able to induce an activating response to cellular damage repair
processes. This perspective aims to draw the attention of researchers to these aspects in an attempt to
address this gap in understanding.
Exosomes from Different Sources in Promoting Wound Healing
Authors: Yi Ruiqi , Yang Xiaolu , Tian Ya , Zhang Fen , Anayyat Umer , Zhang Xiaoting , Xiaomei Wang 
Published: January 2025

Abstract:
Exosomes are nanoscale extracellular vesicles with various biological activities that can accelerate
wound healing by regulating inflammatory responses, promoting cell proliferation and angiogenesis,
and other mechanisms. Among them, plant and animal exosomes have demonstrated unique
advantages due to their biological characteristics. Plant exosomes have gradually become a research
hotspot due to their wide source, high biosafety, and low production cost, demonstrating significant
pro-healing potential. In addition, animal exosomes, especially human-derived stem cell exosomes,
have shown excellent results in promoting wound repair and have become the focus of clinical research.
This review explores the mechanism of action of these exosomes and their application prospects
in wound healing, providing theoretical support for future research and clinical applications.
Evaluasi Indeks Kinerja Sistem Irigasi di Daerah Irigasi Palasari Kabupaten Jembrana Menggunakan Aplikasi e-PAKSI
Authors: Novia Lelyana  , I Gusti Ngurah Kerta Arsana  , I Putu Gustave Suryantara Pariartha  
Published: January 2025

Daerah Irigasi Palasari merupakan salah satu daerah irigasi di Kabupaten Jembrana, Provinsi Bali, dengan luas fungsional sebesar 564 Ha. Sesuai dengan amanat Peraturan Menteri Pekerjaan Umum dan Perumahan Rakyat Republik Indonesia Nomor 12/PRT/M/2015 tentang Eksploitasi dan Pemeliharaan Jaringan Irigasi bahwa perlu dilakukan pengelolaan aset irigasi dan evaluasi kinerja sistem irigasi. Studi ini bertujuan untuk mengetahui indeks kinerja sistem irigasi di Daerah Irigasi yang nantinya dapat dijadikan acuan dalam pengambilan keputusan dalam pengelolaan irigasi di masa mendatang. Metode yang digunakan untuk memperoleh indeks tersebut adalah dengan melakukan survei Pengelolaan Aset Irigasi (PAI) dan Indeks Kinerja Sistem Irigasi (IKSI) melalui penelusuran jaringan irigasi menggunakan aplikasi Android e-PAKSI yang berbasis web.
Berdasaran hasil survei PAI dan IKSI Daerah Irigasi Palasari menggunakan aplikasi e-PAKSI, diperoleh nilai indeks kondisi operasi dan pemeliharaan jaringan irigasi dan nonjaringan irigasi berdasarkan penilaian enam komponen yang ada di Daerah Irigasi Palasari yaitu prasarana fisik sebesar 24,65%, produktivitas tanam sebesar 12,13%, sarana penunjang sebesar 4,75%, organisasi personalia sebesar 6,50%, dokumentasi sebesar 1,90%, dan P3A sebesar 7,00%. Sehingga, nilai IKSI total untuk Daerah Irigasi Palasari adalah sebesar 56,92%, dengan nilai IKSI dalam interval 55% - 69% menunjukkan bahwa Daerah Irigasi Palasari memiliki kinerja kurang dan perlu perhatian.
Development of a Metacognition-Based E-Book Using 3D Pageflip to Enhance Polymer Chemistry Literacy in Coordination Polymerization Reactions
Authors: Yusnaidar Yusnaidar , Syahri Wilda 
Published: January 2025

The development of a metacognition-based e-book using 3D Pageflip on coordination polymerization reaction materials aims to enhance students' polymer chemistry literacy. The Polymer course in the Chemistry Education Study Program covers various polymerization reactions essential for students in today's educational era. Polymers, including plastics, Styrofoam, nylon, and others, are crucial materials in various aspects of life. Due to the abstract nature of this subject, theoretical explanations are challenging for students to understand. A metacognition-based e-book is expected to assist students in self-learning, increasing flexibility in understanding polymerization reaction materials. This study employs the ADDIE development model (Analysis, Design, Development, Implementation, and Evaluation) and produces an e-book containing text, animations, videos, and music. Validation by experts indicates that the e-book is excellent in terms of content and design. Implementation among students shows that this e-book is effective in enhancing the understanding of coordination polymerization reactions. Thus, the e-book is expected to facilitate learning and improve students' polymer chemistry literacy.
Keyword: Science literacy, polymer chemistry, polymerization reaction, coordination polymerization.