bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scaling factors for 1-D ground response amplification in a soft soil basin
Authors: Apriadi Dedi , Mandhany Anggariano , Sahadewa Andhika , Basarah Yuamar I. , Sengara Wayan , Hakim Abi Maulana 
Published: January 2024

Basin presence is believed to affect the ground surface response due to earthquakes, particularly in areas around the basin edge. Previous studies showed that 1-D and 2-D wave propagation analyses resulted in significant differences in amplification at the basin edge. However, the link between 1-D and 2-D responses has not been studied for engineering practices. In practical application, seismic studies were commonly performed using 1-D analysis, for example, to develop a city micro-zonation map. Based on practical considerations, it is necessary to estimate the scaling factor for the 1-D analysis by considering the basin presence, particularly for one containing soft soil. There are three stages carried out in this study. The first stage: collecting data on some basin geometries for the 2-D modeling references and then defining selected site class and input motions. The second stage: modeling 1-D and 2-D wave propagation using D-MOD and Fast Lagrangian Analysis of Continua (FLAC), respectively. The third stage: comparing spectral acceleration resulting from the 1-D and 2-D analyses to obtain the scaling factors. This research studied and reported the relationship between PGA values varied as 0.2 g, 0.3 g, 0.4 g, and 0.5 g, basin geometry (e.g., the angle was set to 5°, 10°, 15°, 30°, and 45°, with depth and width variations of 0.0125, 0.025, 0.05, 0.075, 0.1, 0.2, and 0.4, while the basin width was adjusted to 500 m, 1 km, 2 km, and 4 km), and the spectral acceleration in several observation points on the ground surface. Based on this evaluation, a series of scaling factors are proposed. These factors can be used for spectral acceleration from available hazard maps, commonly developed based on 1-D analysis. The application example of this scaling factor is presented in this study, using the Bandung Basin case.
Engagement of adolescents with ADHD in a narrative-centered game-based behavior change environment to reduce alcohol use
Authors: Pugatch Marianne , Blum Nathan J. , Barbaresi William J. , Rowe Jonathan , Berna Mark , Hennigan Sean , Giovanelli Alison , Penilla Carlos , Tebb Kathleen P. , Mott Megan , Kumaran Vikram , Buckelew Sara , Lester James C. , Ozer Elizabeth 
Published: January 2024

BackgroundAttention deficit hyperactivity disorder (ADHD) affects about 13% of adolescents and is associated with substance use-related morbidity and mortality. While evidence on effective interventions to reduce alcohol use among adolescents with ADHD is limited, parent-teen communication about alcohol use has been found to be protective. Other approaches, such as educational interventions, hold promise to reduce alcohol-related harms in adolescents with ADHD. Digital technology offers an innovative approach to health behavior change, expanding access to services and may promote learning for neurodiverse youth, including teens with ADHD. INSPIRE, a narrative-centered game-based behavior change environment designed to promote self-regulation and self-efficacy to prevent risky alcohol use has been found to engage a general adolescent population. The goals of this pilot study are (1) to examine the engagement of youth with ADHD in INSPIRE; and (2) to examine if INSPIRE fosters parent-teen communication.MethodAdolescents diagnosed with ADHD aged 14–16 were recruited from developmental medicine clinics and invited to a focus group offered via Zoom. Participants completed a pre-survey, interacted with the INSPIRE game, and answered a post-survey as well as open-ended questions about their gaming experience during the focus group. Engagement was measured through both self-report using subscales from the User Engagement Scale and computer data; survey and qualitative data collected information on parent-teen communication. Univariate statistics described adolescent characteristics, Rank-sum and Fisher’s exact tests examined relationships among variables, and qualitative analysis identified themes in open-ended questions.ResultsOf adolescent participants (N = 40), 45% identified as female, 17.5% Black, 7.5%, Hispanic, and 62.5% White. Post-survey mean engagement subscales of Usability (on a 5-point scale) was 3.67 (SD = 0.74), and Satisfaction was 3.63 (SD = 0.75). Computer data indicated that participants played the game for a median of 24 min. Adolescents shared that playing the game strengthened refusal skills and their ability to navigate social gatherings with alcohol. Post-survey, 63% planned to share information from INSPIRE with a parent.ConclusionFindings suggest that INSPIRE may support facilitating youth with ADHD to learn the developmental competencies needed to mitigate risk and thrive. INSPIRE warrants further testing to explore its impact on alcohol use in youth with ADHD.
Impacts of ovarian reserve on conservative treatment for endometrial cancer and atypical hyperplasia
Authors: Wu Pengfei , Shan Weiwei , Xue Yu , Wang Lulu , Liu Sijia , Chen Xiaojun , Luo Xuezhen 
Published: January 2024

ObjectivesReal-world data indicated that some endometrial atypical hyperplasia (EAH) and early endometrial carcinoma (EEC) patients of fertility preservation had a normal ovarian reserve, while some had a decreased ovarian reserve (DOR). This study was designed to investigate the effect of baseline ovarian reserve on the treatment of EAH and EEC patients who ask for preservation of fertility.MethodsThis was a prospective cohort study conducted at a single university-affiliated fertility center. A total of 102 EAH and EEC patients who received fertility-preserving treatment between March 2019 and August 2020 were included and divided into a DOR group (n=22) and a non-DOR group (n=80).ResultsThe 32-week CR rate of the non-DOR group was significantly higher than that of the DOR group (60.3% vs. 33.3%, P =0.028). The DOR group had a longer treatment duration to achieve CR than the non-DOR group (40.07 vs. 29.71 weeks, P=0.008, HR: 0.54, 95% CI: 0.36–0.86). Multivariate logistic regression analyses demonstrated that DOR (OR: 0.35, 95% CI: 0.13–0.99, P=0.049) and BMI ≥25 kg/m2 (OR: 0.40, 95% CI: 0.17–0.92, P=0.031) were negatively associated with 32-week CR.ConclusionsDecreased baseline ovarian reserve is negatively correlated with the efficacy of fertility-preserving treatment in EAH and EEC patients, as this group has a lower CR rate and a longer treatment duration to achieve CR than those without DOR.
Multi-omics analysis reveals cuproptosis and mitochondria-based signature for assessing prognosis and immune landscape in osteosarcoma
Authors: Jia Chenguang , Liu Mei , Yao Liming , Zhao Fangchao , Liu Shuren , Li Zhuo , Han Yongtai 
Published: January 2024

BackgroundOsteosarcoma (OSA), the most common primary mesenchymal bone tumor, is a health threat to children and adolescents with a dismal prognosis. While cuproptosis and mitochondria dysfunction have been demonstrated to exert a crucial role in tumor progression and development, the mechanisms by which they are regulated in OSA still await clarification.MethodsTwo independent OSA cohorts containing transcriptome data and clinical information were collected from public databases. The heterogeneity of OSA were evaluated by single cell RNA (scRNA) analysis. To identify a newly molecular subtype, unsupervised consensus clustering was conducted. Cox relevant regression methods were utilized to establish a prognostic gene signature. Wet lab experiments were performed to confirm the effect of model gene in OSA cells.ResultsWe determined 30 distinct cell clusters and assessed OSA heterogeneity and stemness scRNA analysis. Then, univariate Cox analysis identified 24 candidate genes which were greatly associated with the prognosis of OSA. Based on these prognostic genes, we obtained two molecular subgroups. After conducting step Cox regression, three model genes were selected to construct a signature showing a favorable performance to forecast clinical outcome. Our proposed signature could also evaluate the response to chemotherapy and immunotherapy of OSA cases.ConclusionWe generated a novel risk model based on cuproptosis and mitochondria-related genes in OSA with powerful predictive ability in prognosis and immune landscape.
The incorporation of the 3HP regimen for tuberculosis preventive treatment in the Brazilian health system: a secondary-database nationwide analysis
Authors: Mendes Luiz Villarinho Pereira , Trajman Anete , Campos Mônica Rodrigues , Correa Marilena Cordeiro Dias Villela , Osorio-de-Castro Claudia Garcia Serpa 
Published: January 2024

IntroductionThe recommendation of rifampin-based shorter - and safer – regimens for tuberculosis preventive treatment (TPT) is progressively replacing monotherapy with isoniazid by different countries. The Brazilian Ministry of Health (MoH) approved the incorporation of the Rifapentine + isoniazid regimen (3HP) at the end of 2020, with free distribution in the Brazilian Unified Health System (SUS) started from the last quarter of 2021. The objectives were to describe the implementation of the IL-TB System (Information System of TPT Notification) and uptake of Rifapentine + isoniazid (3HP) and Isoniazid (6H or 9H) in Brazil.MethodsA quantitative observational and descriptive was performed using the IL-TB National System as the main data source, from January 2018 to December 2022.Results and discussionThere was a steady increase of the number of TPT prescription quarterly throughout the period, which reflects the implementation of the system itself and the progressive adherence of the health system to the non-compulsory notification of new TPT. The substitution of isoniazid (6H or 9H) by 3HP is progressing. The 3HP regimen represented less than 4% of the total administered by the end of 2021, reaching around 30% in the second half of 2022 and 40% in the last quarters of 2022. The study points not only to the need to expand TPT in the country, but also to accelerate 3HP uptake and to encourage the municipalities to notify to the IL-TB system, since there is still a high level of underreporting.
The role of gut microbiota in the occurrence and progression of non-alcoholic fatty liver disease
Authors: Mai Huanzhuo , Yang Xing , Xie Yulan , Zhou Jie , Wang Qing , Wei Yiru , Yang Yuecong , Lu Dongjia , Ye Li , Cui Ping , Liang Hao , Huang Jiegang 
Published: January 2024

BackgroundNon-alcoholic fatty liver disease (NAFLD) is the most prevalent cause of chronic liver disease worldwide, and gut microbes are associated with the development and progression of NAFLD. Despite numerous studies exploring the changes in gut microbes associated with NAFLD, there was no consistent pattern of changes.MethodWe retrieved studies on the human fecal microbiota sequenced by 16S rRNA gene amplification associated with NAFLD from the NCBI database up to April 2023, and re-analyzed them using bioinformatic methods.ResultsWe finally screened 12 relevant studies related to NAFLD, which included a total of 1,189 study subjects (NAFLD, n = 654; healthy control, n = 398; obesity, n = 137). Our results revealed a significant decrease in gut microbial diversity with the occurrence and progression of NAFLD (SMD = −0.32; 95% CI −0.42 to −0.21; p &lt; 0.001). Alpha diversity and the increased abundance of several crucial genera, including Desulfovibrio, Negativibacillus, and Prevotella, can serve as an indication of their predictive risk ability for the occurrence and progression of NAFLD (all AUC &gt; 0.7). The occurrence and progression of NAFLD are significantly associated with higher levels of LPS biosynthesis, tryptophan metabolism, glutathione metabolism, and lipid metabolism.ConclusionThis study elucidated gut microbes relevance to disease development and identified potential risk-associated microbes and functional pathways associated with NAFLD occurrence and progression.
Probiotics in milk replacer affect the microbiome of the lung in neonatal dairy calves
Authors: McDaneld Tara G. , Eicher Susan D. , Dickey Aaron , Kritchevsky Janice E. , Bryan Keith A. , Chitko-McKown Carol G. 
Published: January 2024

IntroductionProbiotics have been investigated for their many health benefits and impact on the microbiota of the gut. Recent data have also supported a gut–lung axis regarding the bacterial populations (microbiomes) of the two locations; however, little research has been performed to determine the effects of oral probiotics on the microbiome of the bovine respiratory tract. We hypothesized that probiotic treatment would result in changes in the lung microbiome as measured in lung lavage fluid. Our overall goal was to characterize bacterial populations in the lungs of calves fed probiotics in milk replacer and dry rations from birth to weaning.MethodsA group of 20 dairy calves was split into two treatment groups: probiotic (TRT; N = 10, milk replacer +5 g/d probiotics; Bovamine Dairy, Chr. Hansen, Inc., Milwaukee, WI) and control (CON; N = 10, milk replacer only). On day 0, birth weight was obtained, and calves were provided colostrum as per the dairy SOP. On day 2, probiotics were added to the milk replacer of the treated group and then included in their dry ration. Lung lavages were performed on day 52 on five random calves selected from each treatment group. DNA was extracted from lavage fluid, and 16S ribosomal RNA (rRNA) gene hypervariable regions 1–3 were amplified by PCR and sequenced using next-generation sequencing (Illumina MiSeq) for the identification of the bacterial taxa present. Taxa were classified into both operational taxonomic units (OTUs) and amplicon sequence variants (ASVs).ResultsOverall, the evaluation of these samples revealed that the bacterial genera identified in the lung lavage samples of probiotic-fed calves as compared to the control calves were significantly different based on the OTU dataset (p &lt; 0.05) and approached significance for the ASV dataset (p &lt; 0.06). Additionally, when comparing the diversity of taxa in lung lavage samples to nasal and tonsil samples, taxa diversity of lung samples was significantly lower (p &lt; 0.05).DiscussionIn conclusion, analysis of the respiratory microbiome in lung lavage samples after probiotic treatment provides insight into the distribution of bacterial populations in response to oral probiotics and demonstrates that oral probiotics affect more than the gut microbiome.
Lethal severe fever with thrombocytopenia syndrome virus infection causes systemic germinal centre failure and massive T cell apoptosis in cats
Authors: Sakai Yusuke , Mura Serina , Kuwabara Yuko , Kagimoto Saya , Sakurai Masashi , Morimoto Masahiro , Park Eun-sil , Shimojima Masayuki , Nagata Noriyo , Ami Yasushi , Yoshikawa Tomoki , Iwata-Yoshikawa Naoko , Fukushi Shuetsu , Watanabe Shumpei , Kurosu Takeshi , Okutani Akiko , Kimura Masanobu , Imaoka Koichi , Saijo Masayuki , Morikawa Shigeru , Suzuki Tadaki , Maeda Ken 
Published: January 2024

IntroductionSevere fever with thrombocytopenia syndrome (SFTS) is a fatal viral disease characterized by high fever, thrombocytopenia, leukopenia, and multi-organ haemorrhage. Disruption of the humoral immune response and decreased lymphocyte numbers are thought to contribute to the disease severity. These findings have been obtained through the analysis of peripheral blood leukocytes in human patients, whereas analysis of lymph nodes has been limited. Thus, in this study, we characterized the germinal centre response and apoptosis in the lymph nodes of cats with fatal SFTS, because SFTS in cats well mimics the pathology of human SFTS.MethodsLymph node tissue sections collected during necropsy from seven fatal SFTS patients and five non-SFTS cases were used for histopathological analysis. Additionally, lymph node tissue sections collected from cats with experimental infection of SFTS virus (SFTSV) were also analysed.ResultsIn the lymphoid follicles of cats with SFTS, a drastic decrease in Bcl6- and Ki67-positive germinal centre B cells was observed. Together, the number of T cells in the follicles was also decreased in SFTS cases. In the paracortex, a marked increase in cleaved-caspase3 positivity was observed in T cells. These changes were independent of the number of local SFTS virus-positive cell. Furthermore, the analysis of cats with experimental SFTSV infection revealed that the intrafollicular Bcl6- and CD3-positive cell numbers in cats with low anti-SFTSV antibody production were significantly lower than those in cats with high anti-SFTSV antibody production.DiscussionThese results suggest that dysfunction of the humoral response in severe SFTS was caused by the loss of germinal centre formation and massive apoptosis of T cells in the lymph nodes due to systemically circulating viruses.
Retrograde procedural memory is impaired in people with Parkinson’s disease with freezing of gait
Authors: Pauly Laure , Pauly Claire , Hansen Maxime , Schröder Valerie E. , Rauschenberger Armin , Leist Anja K. , Krüger Rejko 
Published: January 2024

BackgroundFreezing of gait (FOG), is associated with impairment of different cognitive functions. Previous studies hypothesized that FOG may be due to a loss of automaticity.Research questionTo explore whether FOG is associated with impairment in cognitive functions, focusing on retrograde procedural memory, the memory responsible for the automatic, implicit stored procedures that have been acquired in earlier life stages.MethodsIn this cross-sectional, case–control study, 288 people with typical Parkinson’s disease (PD) from the Luxembourg Parkinson’s Study were assigned to Freezers (FOG+) and non-Freezers (FOG−) based on the MDS-UPDRS 2.13 (self-reported FOG episodes) and 3.11 (FOG evaluated by clinicians during gait assessment). Both groups were matched on age, sex and disease duration. Global cognition (MoCA), retrograde procedural memory and visuo-constructive abilities (CUPRO), psychomotor speed and mental flexibility (TMT) were assessed. Furthermore, we repeated our analyses by additionally controlling for depression (BDI-I).ResultsBesides lower global cognition (MoCA; p = 0.007) and mental flexibility (TMT-B and Delta-TMT; p &lt; 0.001), FOG+ showed a lower performance in retrograde procedural memory (CUPRO-IS1; p &lt; 0.001) compared to FOG−. After controlling additionally for depression, our main outcome variable CUPRO-IS1 remained significantly lower in FOG+ (p = 0.010).ConclusionOur findings demonstrated that besides lower global cognition and mental flexibility scores, FOG+ showed lower performance in retrograde procedural memory compared to matched FOG-control patients, even when accounting for factors such as age, sex, disease duration or depression.SignificanceIn the context of limited treatment options, especially for non-invasive therapeutic approaches, these insights on procedural memory and FOG may lead to new hypotheses on FOG etiology and consequently the development of new treatment options.
Age-related changes in the primary auditory cortex of newborn, adults and aging bottlenose dolphins (Tursiops truncatus) are located in the upper cortical layers
Authors: Graïc Jean-Marie , Corain Livio , Finos Livio , Vadori Valentina , Grisan Enrico , Gerussi Tommaso , Orekhova Ksenia , Centelleghe Cinzia , Cozzi Bruno , Peruffo Antonella 
Published: January 2024

IntroductionThe auditory system of dolphins and whales allows them to dive in dark waters, hunt for prey well below the limit of solar light absorption, and to communicate with their conspecific. These complex behaviors require specific and sufficient functional circuitry in the neocortex, and vicarious learning capacities. Dolphins are also precocious animals that can hold their breath and swim within minutes after birth. However, diving and hunting behaviors are likely not innate and need to be learned. Our hypothesis is that the organization of the auditory cortex of dolphins grows and mature not only in the early phases of life, but also in adults and aging individuals. These changes may be subtle and involve sub-populations of cells specificall linked to some circuits.MethodsIn the primary auditory cortex of 11 bottlenose dolphins belonging to three age groups (calves, adults, and old animals), neuronal cell shapes were analyzed separately and by cortical layer using custom computer vision and multivariate statistical analysis, to determine potential minute morphological differences across these age groups.ResultsThe results show definite changes in interneurons, characterized by round and ellipsoid shapes predominantly located in upper cortical layers. Notably, neonates interneurons exhibited a pattern of being closer together and smaller, developing into a more dispersed and diverse set of shapes in adulthood.DiscussionThis trend persisted in older animals, suggesting a continuous development of connections throughout the life of these marine animals. Our findings further support the proposition that thalamic input reach upper layers in cetaceans, at least within a cortical area critical for their survival. Moreover, our results indicate the likelihood of changes in cell populations occurring in adult animals, prompting the need for characterization.
Vehicle re-identification method based on multi-attribute dense linking network combined with distance control module
Authors: Sun Xiaoming , Chen Yan , Duan Yan , Wang Yongliang , Zhang Junkai , Su Bochao , Li Li 
Published: January 2024

IntroductionVehicle re-identification is a crucial task in intelligent transportation systems, presenting enduring challenges. The primary challenge involves the inefficiency of vehicle re-identification, necessitating substantial time for recognition within extensive datasets. A secondary challenge arises from notable image variations of the same vehicle due to differing shooting angles, lighting conditions, and diverse camera equipment, leading to reduced accuracy. This paper aims to enhance vehicle re-identification performance by proficiently extracting color and category information using a multi-attribute dense connection network, complemented by a distance control module.MethodsWe propose an integrated vehicle re-identification approach that combines a multi-attribute dense connection network with a distance control module. By merging a multi-attribute dense connection network that encompasses vehicle HSV color attributes and type attributes, we improve classification rates. The integration of the distance control module widens inter-class distances, diminishes intra-class distances, and boosts vehicle re-identification accuracy.ResultsTo validate the feasibility of our approach, we conducted experiments using multiple vehicle re-identification datasets. We measured various quantitative metrics, including accuracy, mean average precision, and rank-n. Experimental results indicate a significant enhancement in the performance of our method in vehicle re-identification tasks.DiscussionThe findings of this study provide valuable insights into the application of multi-attribute neural networks and deep learning in the field of vehicle re-identification. By effectively extracting color information from the HSV color space and vehicle category information using a multi-attribute dense connection network, coupled with the utilization of a distance control module to process vehicle features, our approach demonstrates improved performance in vehicle re-identification tasks, contributing to the advancement of smart city systems.