bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Liquiritin on Mitochondrial Dynamics in Cerebral Ischemia–Reperfusion Injury by Regulating Nurr1 via the YAP-INF2-Mitochondrial Fission Pathway
Authors: Fan Yao , Zhang Xi , Zhou Hui , Zhang Zhanwei 
Published: December 2023

AbstractThis study investigated whether liquiritin can alleviate cerebral ischemia–reperfusion injury by regulating Nurr1 to mediate mitochondrial homeostasis. SH-SY5Y cells were subjected to glucose deprivation and reperfusion to establish a cerebral ischemia–reperfusion injury model in vitro. Cell viability and apoptosis were then determined using a cell counting kit and flow cytometry analysis. The degree of mitochondrial swelling was evaluated using a cell mitochondria isolation kit. Reactive superoxide generation, mitochondrial membrane potential, adenosine triphosphate (ATP) content, and mitochondrial ultrastructure were analyzed using dihydroethidium, JC-1 (5,5′,6,6′-tetrachloro1,1′,3,3′-tetramethylbenzimidazolylcarbocyanine iodide), luciferase-based ATP bioluminescent assays, and transmission electron microscopy, respectively. Quantitative reverse transcription PCR and western blot assays were conducted to detect levels of mitochondrial fission-related factors. Glucose deprivation and reperfusion exposure significantly reduced the viability and induced apoptosis of SH-SY5Y cells, indicating that glucose deprivation and reperfusion exposure successfully induced cerebral ischemia–reperfusion injury. Glucose deprivation and reperfusion exposure also increased the degree of mitochondrial swelling, promoted an increase in superoxide, and decreased mitochondrial membrane potential and ATP enzyme levels. Cerebral ischemia–reperfusion injury also significantly increases Drp1 and Fis1 protein expression, reduces mitofusin-2 and optic atrophy 1 levels, increases nuclear receptor-related 1 and inverted formin-2 expression, and decreases yes-associated protein expression. Electron microscopy further revealed sparse mitochondria and broken cristae. However, these findings were reversed by liquiritin in a dose-dependent manner and were further abolished after carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone treatment. Our study suggests that the protective effects of liquiritin on cerebral ischemia–reperfusion injury are linked to nuclear receptor-related 1 upregulation, followed by the regulation of yes-associated protein-inverted formin-2-mitochondrial fission pathways. Liquiritin may represent a novel therapeutic agent for treating cerebral ischemia–reperfusion injury.
Graphical Abstract
Heatstroke presentations to urban hospitals during BC’s extreme heat event: lessons for the future
Authors: Gossack-Keenan Kira , Yeom David Seonguk , Kanu Josephine , Hau Jeffrey P. , Rosychuk Rhonda J. , Clark Dylan , Bola Rajan , Tze Caris , Niosco Chris , Emery Hayley , Yeung Phillip , Hohl Corinne M. 
Published: December 2023

Abstract
Background
Climate change is leading to more extreme heat events in temperate climates that typically have low levels of preparedness. Our objective was to describe the characteristics, treatments, and outcomes of adults presenting to hospitals with heatstroke during BC’s 2021 heat dome.

Methods
We conducted a review of consecutive adults presenting to 7 hospitals in BC’s Lower Mainland. We screened the triage records of all patients presenting between June 25th and 30th, 2021 for complaints related to heat, and reviewed the full records of those who met heatstroke criteria. Our primary outcome was in-hospital mortality. We used Mann–Whitney U tests and logistic regression to investigate associations between patient and treatment factors and mortality.

Results
Among 10,247 consecutive presentations to urban hospitals during the extreme heat event, 1.3% (139; 95% confidence intervals [CI] 1.1–1.6%) met criteria for heatstroke. Of heatstroke patients, 129 (90.6%) were triaged into the two highest acuity levels. Patients with heatstroke had a median age of 84.4 years, with 122 (87.8%) living alone, and 101 (84.2%) unable to activate 911 themselves. A minority (< 5, < 3.6%) of patients presented within 48 h of the onset of extreme heat. Most patients (107, 77.0%) required admission, and 11.5% (16) died in hospital. Hypotension on presentation was associated with mortality (odds ratio [OR] 5.3).

Interpretation
Heatstroke patients were unable to activate 911 themselves, and most presented with a 48-h delay. This delay may represent a critical window of opportunity for pre-hospital and hospital systems to prepare for the influx of high-acuity resource-intensive patients.
Underwater image enhancement via histogram similarity-oriented color compensation complemented by multiple attribute adjustment
Authors: Wang Hao , Frery Alejandro C. , Li Mingjie , Ren Peng 
Published: December 2023

AbstractUnderwater images are often influenced by color casts, low contrast, and blurred details. We observe that images taken in natural settings typically have similar histograms across color channels, while underwater images do not. To improve the natural appearance of an underwater image, it is critical to improve the histogram similarity across its color channels. To address this problem, we develop a histogram similarity-oriented color compensation method that corrects color casts by improving the histogram similarity across color channels in the underwater image. In addition, we apply the multiple attribute adjustment method, including max-min intensity stretching, luminance map-guided weighting, and high-frequency edge mask fusion, to enhance contrast, saturation, and sharpness, effectively addressing problems of low contrast and blurred details and eventually enhancing the overall appearance of underwater images. Particularly, the method proposed in this work is not based on deep learning, but it effectively enhances a single underwater image. Comprehensive empirical assessments demonstrated that this method exceeds state-of-the-art underwater image enhancement techniques. To facilitate public assessment, we made our reproducible code available at https://gitee.com/wanghaoupc/UIE_HS2CM2A.
The Stability Transition From Stable to Unstable Frictional Slip With Finite Pore Pressure
Authors: Affinito R. , Wood C. , Marty S. , Elsworth D. , Marone C. 
Published: December 2023

AbstractPore fluids are ubiquitous throughout the lithosphere and are commonly invoked as the cause of induced seismicity and slow earthquakes. We perform lab experiments to address these questions for drained fault conditions and low pore pressure. We shear simulated faults at effective normal stress of 20 MPa and pore pressures Pp from 1 to 4 MPa. We document the full range of lab earthquake behaviors from slow slip to elasto‐dynamic rupture and show that slow slip can be explained by the slip rate dependence of the critical rheologic stiffness without dilatancy hardening or other fluid effects. Our fault permeabilities ranges from 10−18 to 10−17 m2 with an initial porosity of 0.1 and estimated fluid diffusion time ≈1 s. Slow slip and quasi‐dynamic fault motion may arise from high Pp at higher pressures but dilatancy strengthening is not a general requirement.
Characteristics of Warm‐Season Mesoscale Convective Systems Over the Yangtze–Huaihe River Basin (YHR): Comparison Between Radar and Satellite
Authors: Lu Yutong , Tang Jianping , Xu Xin , Tang Ying , Fang Juan 
Published: December 2023

AbstractMesoscale convective systems (MCSs) are crucial in modifying the water cycle and frequently induce high‐impact weather events over eastern China. Radar and Climate Prediction Center (CPC)‐4 km satellite‐derived infrared cloud top temperature (Tb) data were used to thoroughly analyze the long‐term climatology of MCSs over eastern China, particularly in the Yangtze–Huaihe River Basin (YHR) in the warm season from 2013 to 2018. For the first time, we contrasted the effects of data set selection and threshold setting on research outcomes. The large‐scale environments of MCSs initiation were also investigated using the latest global reanalysis data ERA5. It is found that striction of thresholds, including duration, reflectivity/Tb, area, and linearity, would lead to a greater proportion of early‐morning MCSs. Satellite‐identified MCSs differed from radar‐derived ones, exhibiting afternoon diurnal peaks, faster movement speeds, longer travel distances, and expansive impact areas. The center of MCS and related precipitation shifted northward from Pre‐Meiyu to Post‐Meiyu seasons, contributing to up to 20% of total rainfall, with most MCSs moving along eastward trajectories. MCSs typically had the most substantial impact in the Meiyu season because of the most prolonged duration, largest convective core area, and strongest precipitation intensity. Warm‐season MCSs initiated ahead of midlevel troughs and were related to strong anomalous low‐level convergence and midlevel upward. The circulation anomalies were the strongest in the Pre‐Meiyu season among the three subseasons, with most moisture sourced from the southwest.
Climate Change Response of Tropical Atlantic Clouds in a Kilometer‐Resolution Model
Authors: Heim Christoph , Schär Christoph 
Published: December 2023

AbstractEstimates of the tropical cloud feedback of global climate models (GCMs) show a large inter‐model spread due to the small‐scale nature of convective cloud processes. Estimates from large‐eddy simulations (LES) are more consistent among themselves, but difficult to scale to the climate system. Here we consider a compromise between GCMs and LES, and study how tropical clouds over the Atlantic respond to a climate perturbation in a regional kilometer‐resolution model. We perform two 4‐year‐long simulations at 3.3 km horizontal grid spacing with the limited‐area model COSMO on a 9,000 × 7,000 km2 domain covering the tropical Atlantic: a control simulation and a climate change simulation using the pseudo‐global warming approach. In a previous publication we have demonstrated that this approach yields a credible representation of the tropical climate without the double‐ITCZ bias commonly seen in GCMs. Here we address the cloud feedback and find a reduction of ITCZ high‐cloud cover resulting in a negative longwave cloud feedback similar to the CMIP6 ensemble mean. We find a reduction of stratocumulus clouds, which we argue is primarily a thermodynamic response. More surprisingly, the shallow cumulus cloud cover over the West Atlantic increases, which we argue is due to increased stability resulting in weaker entrainment and a more humid boundary layer. The mean shortwave cloud feedback over the tropical Atlantic is positive, comparable to the CMIP6 ensemble mean. The emerging estimate of the total cloud‐radiative feedback over the Atlantic is slightly negative, consistent with CMIP6 GCMs, but it is substantially stronger than that of the driving GCM.
Contributions of Air‐Sea Coupling to Northward‐Propagating Intraseasonal Convection Over the South China Sea
Authors: Gao Yingxia , Hsu Pang‐Chi 
Published: December 2023

AbstractNorthward‐propagating convection associated with intraseasonal oscillation (ISO) over the South China Sea (SCS) is closely linked to the evolution of the East China rainy season. In this study, we quantitatively examined the contribution of air‐sea coupling to northward propagation of intraseasonal convection over the SCS based on observational diagnosis and model experiments. While oceanic feedback plays a limited role in northward propagation of Indian Ocean and western Pacific ISO, its positive effect on SCS ISO propagation is much larger and nearly comparable with that of atmospheric internal dynamics over the central SCS. Warm intraseasonal sea‐surface temperature (SST) anomalies ahead of the convection induce boundary‐layer convergence and surface turbulent fluxes, largely favoring growth of moist static energy to the north of the ISO convection. Conversely, weak negative SST anomalies induce divergence and reduced fluxes over the convective center. The underlying SST anomalies strongly promote northward propagation of SCS ISO, but destroy the maintenance of deep convection. We then verified these oceanic feedbacks using atmosphere‐only (SPA) and atmosphere–ocean‐mixed‐layer coupled (SPK) configurations of the Super‐Parameterized Community Atmospheric Model. The SPA simulations produced stronger intraseasonal convection but weaker propagation signals than the SPK simulations, confirming the positive contribution of intraseasonal SST to ISO propagation observed over the SCS.
The effectiveness of malaria camps as part of the malaria control program in Odisha, India
Authors: Ompad Danielle C. , Padhan Timir K. , Kessler Anne , Tozan Yesim , Jones Abbey M. , van Eijk Anna Maria , Sullivan Steven A. , Haque Mohammed A. , Pradhan Madan Mohan , Mohanty Sanjib , Carlton Jane M. , Sahu Praveen K. 
Published: December 2023

AbstractDurgama Anchalare Malaria Nirakaran (DAMaN) is a multi-component malaria intervention for hard-to-reach villages in Odisha, India. The main component, malaria camps (MCs), consists of mass screening, treatment, education, and intensified vector control. We evaluated MC effectiveness using a quasi-experimental cluster-assigned stepped-wedge study with a pretest–posttest control group in 15 villages: six immediate (Arm A), six delayed (Arm B), and three previous interventions (Arm C). The primary outcome was PCR + Plasmodium infection prevalence. The time (i.e., baseline vs. follow-up 3) x study arm interaction term shows that there were statistically significant lower odds of PCR + Plasmodium infection in Arm A (AOR = 0.36, 95% CI = 0.17, 0.74) but not Arm C as compared to Arm B at the third follow-up. The cost per person ranged between US$3–8, the cost per tested US$4–9, and the cost per treated US$82–1,614, per camp round. These results suggest that the DAMaN intervention is a promising and financially feasible approach for malaria control.
Experimental study and simulations of hydrogen cooling effectiveness for aviation PEM fuel cells
Authors: Kösters Till Lennart , von Schweinitz Arne Graf , Heere Michael , Friedrichs Jens , Gao Xin 
Published: December 2023

AbstractProton exchange membrane fuel cells (PEMFCs) are seen as one possible future means of driving the change towards a zero-emission society. In a civil aircraft, fuel cell systems can have multiple potential benefits, such as reduced noise, lowered emissions and higher fuel economy compared to jet aircraft. For controlling the fuel cell temperature, thermal management systems are required which can be optimized for aircraft applications regarding their weight and reliability. In this work, a simplified and light-weight thermal management system relying on hydrogen cooling is presented and analysed. To investigate the feasibility, a test rig and a three-dimensional, singular channel model in ANSYS Fluent were designed. Fuel cell temperature could be maintained within the set threshold in the model and the test rig, thus showing that controlling the fuel cell temperature via the hydrogen reactant flow is a viable alternative thermal management system. Results from the model indicate that both the hydrogen mass flow and hydrogen inlet temperature should be used to control the fuel cell temperature. Furthermore, operating the fuel cell at medium to low current densities is favourable for hydrogen cooling. Future studies will explore alternate flow field designs to facilitate thermal management system relying on hydrogen.
Comparative analysis of methods to reduce activation signature gene expression in PBMCs
Authors: Andriamboavonjy Lovatiana , MacDonald Adam , Hamilton Laura K. , Labrecque Marjorie , Boivin Marie-Noёlle , Karamchandani Jason , Stratton Jo Anne , Tetreault Martine 
Published: December 2023

AbstractPreserving the in vivo cell transcriptome is essential for accurate profiling, yet factors during cell isolation including time ex vivo and temperature induce artifactual gene expression, particularly in stress-responsive immune cells. In this study, we investigated two methods to mitigate ex vivo activation signature gene (ASG) expression in peripheral blood mononuclear cells (PBMCs): transcription and translation inhibitors (TTis) and cold temperatures during isolation. Comparative analysis of PBMCs isolated with TTis revealed reduced ASG expression. However, TTi treatment impaired responsiveness to LPS stimulation in subsequent in vitro experiments. In contrast, cold isolation methods also prevented ASG expression; up to a point where the addition of TTis during cold isolation offered minimal additional advantage. These findings highlight the importance of considering the advantages and drawbacks of different isolation methods to ensure accurate interpretation of PBMC transcriptomic profiles.
Atosiban application in fresh ET cycle is effective for women undergoing repeated embryo implantation failures, especially for advanced-age obese patients
Authors: Li Jie , Mo Sien , Lin Zhong , Shi Qiuling 
Published: December 2023

AbstractTo assess the effect of atosiban in pregnancy outcomes of the fresh embryo transfer (ET), the retrospective cohort study was conducted. Six hundred and eighty-nine cases (using atosiban) and 1377 age and ET cycle-matched controls were collected from the January 2016 to May 2022 to perform the fresh IVF-ET cycle. The essential characteristics and pregnancy outcomes were analyzed. Conditional logistic regression analysis and subgroup analysis were performed. In the whole samples, atosiban had no effects in the pregnancy outcomes. Subgroup analyses suggested that atosiban could improve the clinical pregnancy in more than 3 ET cycles (OR 1.667, 95% CI 1.108–2.509, P = 0.014). Moreover, the improvement of clinical pregnancy was mainly present in the advanced-age women (age ≥ 35 years: OR 1.851, 95% CI 1.136–3.014, P = 0.013), obesity (BMI ≥ 24 kg/m2: OR 2.550, 95% CI 1.105–5.883, P = 0.028) and cleavage stage embryo (D3 embryo: OR 1.721, 95% CI 1.098–2.696, P = 0.018) among the repeated implantation failures (RIF). Atosiban could also improve the live birth for the obese women. Further, in the RIF, atosiban application was strongly recommended for the advanced-age infertility women, who also had the risk of obesity with the implantation of the cleavage stage embryo. In conclusion, atosiban could improve pregnancy outcomes for the advanced-age and obese women in RIF, especially while implanting the cleavage stage embryo in fresh ET cycle.
Exploring the role of electrostatic deposition on inhaled aerosols in alveolated microchannels
Authors: Bessler Ron , Bhardwaj Saurabh , Malka Daniel , Fishler Rami , Sznitman Josué 
Published: December 2023

AbstractLarge amounts of net electrical charge are known to accumulate on inhaled aerosols during their generation using commonly-available inhalers. This effect often leads to superfluous deposition in the extra-thoracic airways at the cost of more efficient inhalation therapy. Since the electrostatic force is inversely proportional to the square of the distance between an aerosol and the airway wall, its role has long been recognized as potentially significant in the deep lungs. Yet, with the complexity of exploring such phenomenon directly at the acinar scales, in vitro experiments have been largely limited to upper airways models. Here, we devise a microfluidic alveolated airway channel coated with conductive material to quantify in vitro the significance of electrostatic effects on inhaled aerosol deposition. Specifically, our aerosol exposure assays showcase inhaled spherical particles of 0.2, 0.5, and 1.1 μm that are recognized to reach the acinar regions, whereby deposition is typically attributed to the leading roles of diffusion and sedimentation. In our experiments, electrostatic effects are observed to largely prevent aerosols from depositing inside alveolar cavities. Rather, deposition is overwhelmingly biased along the inter-alveolar septal spaces, even when aerosols are charged with only a few elementary charges. Our observations give new insight into the role of electrostatics at the acinar scales and emphasize how charged particles under 2 µm may rapidly overshadow the traditionally accepted dominance of diffusion or sedimentation when considering aerosol deposition phenomena in the deep lungs.
RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway
Authors: Zhang Xiaomei , Wang Jian , Li Haiying 
Published: December 2023

AbstractRTKN2 is a new effector protein of Rho GTPase, and has been indicated to be a tumor inhibitor in colon cancer. In this article, we explored the function of RTKN2 in BC cell development. RTKN2 expression in BC tissues and BC cell lines was evaluated by RT-qPCR and Western blot assay. CCK-8, Wound-healing and Transwell assays were carried out to examine the role of RTKN2 knockdown on proliferation, the migratory ability and the invasive ability of BC cells. FCM and Western blot assay were performed to measure the function of RTKN2 silencing on BC cell apoptosis. In addition, the regulatory effect of RTKN2 on Wnt/β-catenin pathway was studied via Western blot assay. RTKN2 expression was elevated in BC tissues and BC cells. Down-regulation of RTKN2 restrained BC cell progression by suppressing cell proliferation, migratory ability, invasive ability, and inducing apoptosis. In addition, reduced of RTKN2 sharply reduced the expressing levels of Wnt3A, β-catenin, C-Myc, and Cyclin D1, suggesting that RTKN2 silencing blocked the motivation of Wnt/β-catenin pathway in BC development. The in vivo experiment also confirmed the inhibitory effect of RTKN2 on BC tumors. Our study confirmed that RTKN2 was highly expressed in BC. Moreover, RTKN2 knockdown suppressed the development of BC through affecting the Wnt/β-catenin pathway. Hence, we deduced that RTKN2 was a possible treatment target for BC.
Growth and developmental outcomes of infants with hypoxic ischemic encephalopathy
Authors: Park Joonsik , Park Sook Hyun , Kim Chloe , Yoon So Jin , Lim Joo Hee , Han Jung Ho , Shin Jeong Eun , Eun Ho Seon , Park Min Soo , Lee Soon Min 
Published: December 2023

AbstractDespite advances in obstetric care, hypoxic ischemic encephalopathy (HIE) remains a significant disease burden. We determined the national trends of HIE prevalence, therapeutic hypothermia (TH) use, mortality, and outcomes from 2012 to 2019. This study included term infants diagnosed with HIE between 2012 and 2019 from the National Health Insurance Service database. The prevalence of HIE was 2.4 per 1000 births without significant change during the period. TH was performed in approximately 6.7% of infants with HIE, and the annual variation ranged from 2.4 to 12.5%. The mortality among all term infants with HIE was 4.6%. The mortality rate among infants with HIE and TH significantly declined from 40 to 16.9% during the eight years. Infants with TH had higher mortality, increased use of inhaled nitric oxide, and more invasive ventilator use, indicating greater disease severity in the TH group. Infants with TH also showed significantly poorer outcomes, including delayed development, cerebral palsy, sensorineural hearing loss, and seizure, compared to infants without TH (p < 0.0001). With the increasing application of TH, mortality and developmental outcomes among infants with HIE have been improving in the past eight years in Korea. Further efforts to improve outcomes should be needed.