bluuebunny/crossref_metadata_2025_split huggingface dataset:
Niche signalling regulates eIF3d1 phosphorylation to promote distinct modes of translation initiation in stem and differentiating cells
Authors: Wang Ruoxu , Roiuk Mykola , Storer Freya , Teleman Aurelio A. , Amoyel Marc 
Published: December 2023

AbstractStem cells have the unique ability among adult cells to give rise to cells of different identities. To do so, they must change gene expression in response to environmental signals. Much work has focused on how transcription is regulated to achieve these changes, however in many cell types, transcripts and proteins correlate poorly, indicating that post-transcriptional regulation is important. To assess how translational control can influence stem cell fate, we use the Drosophila testis as a model. The testis niche secretes a ligand to activate the JAK/STAT pathway in two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs). We find that global translation rates are high in CySCs and decrease during differentiation, and that JAK/STAT signalling regulates translation. To determine how translation was regulated, we knocked down translation initiation factors and found that the cap binding complex, eIF4F, is dispensable in differentiating cells, but is specifically required in CySCs for self-renewal, acting downstream of JAK/STAT activity. Moreover, we identify eIF3d1 as a key regulator of CySC fate, and show that its phosphorylation is critical to maintain CySC self-renewal. We further show that Casein Kinase II, which controls eIF3d1 phosphorylation, is sufficient to restore CySC function in the absence of JAK/STAT. We propose a model in which niche signals regulate a specific translation programme in which only some mRNAs are translated, through regulation of eIF3d phosphorylation. The mechanism we identify allows stem cells to switch between modes of translation, adding a layer of regulation on top of transcription and providing cells with the ability to rapidly change gene expression upon receiving external stimuli.
Ser/Thr Phosphorylation ofMycobacterium tuberculosisType II Rel TA module by Protein Kinase K interferes with toxin neutralization: A novel mode of TA regulation
Authors: Sarah Shafinaz Rahman , Garg Abhishek , Korch Shaleen , Gupta Amita , Malhotra Vandana 
Published: December 2023

AbstractToxin-Antitoxin (TA) modules represent genetic elements implicated in bacterial persistence and antibiotic tolerance. Remarkably,Mycobacterium tuberculosisencodes 90+ TA modules, the majority of which are Type II TA comprising of a toxin component and an antitoxin counterpart that neutralizes the toxin. Upon exposure to stress, the antitoxin is degraded, releasing the toxin which then acts to halt cellular growth. Given that TA modules dictate social behavior of a population, we hypothesize that their regulation must be exquisitely controlled to avoid superfluous growth inhibition and initiation of persistence. However, the regulation and coordination of TA modules is poorly understood. Herein, we describe for the first time, a novel regulatory mechanism for Type II TA modules involving post-translational modification (PTM). Using computational tools, we observed that over 85 % of theM. tuberculosisTA proteins possess potential Ser/Thr phosphosites highlighting them as putative substrates forM. tuberculosisSer/Thr protein kinases (STPK). We demonstrate that members of the RelBE family are subjected toO-phosphorylation by PknK, a stress-responsive growth regulatory STPK. Mass spectrometry confirmed multiple sites of PknK-mediated phosphorylation in the RelJK TA module. To gain insights into the functional impact of this PTM, we conductedin vitrobinding and phenotypic growth studies with the wild type and mutant RelJK proteins. Our findings indicate that phosphorylation of Thr77 residue in RelK toxin compromises its binding to the RelJ antitoxin. These results suggest a potential role forO-phosphorylation in influencing the interaction dynamics of the TA module components.ImportanceBacterial pathogens rely on the phenomenon of persistence as a survival strategy to combat the adverse environmental conditions encountered during infection. As a stochastic process, the driving force(s) that potentiate the formation of persisters in a bacterial population are largely unclear. This study is a step towards the discovery of intricate regulatory mechanisms that coordinate a synchronized TA cellular program. We propose a model wherein the TA module is regulated post translationally, specifically via Ser/Thr phosphorylation disrupting the interaction between the toxin and antitoxin proteins as a mechanism to regulate TA function.
Caulobacter crescentusRNase E condensation contributes to autoregulation and fitness
Authors: Nandana Vidhyadhar , Al-Husini Nadra , Vaishnav Arti , Dilrangi Kulathungage H. , Schrader Jared M. 
Published: December 2023

AbstractRNase E is the most common RNA decay nuclease in bacteria, setting the global mRNA decay rate and scaffolding formation of the RNA degradosome complex and BR-bodies. To properly set the global mRNA decay rate, RNase E fromEscherichia coliand neighboring γ-proteobacteria were found to autoregulate RNase E levels via the decay of its mRNA’s 5’ UTR. While the 5’ UTR is absent from other groups of bacteria in the Rfam database, we identified that the α-proteobacteriumCaulobacter crescentusRNase E contains a similar 5’ UTR structure that promotes RNase E autoregulation. In both bacteria, the C-terminal IDR of RNase E is required for proper autoregulation to occur, and this IDR is also necessary and sufficient for RNase E to phase-separate, generating BR-bodies. Usingin vitropurified RNase E, we find that the IDR’s ability to promote phase-separation correlates with enhanced 5’ UTR cleavage, suggesting that phase-separation of RNase E with the 5’ UTR enhances autoregulation. Finally, using growth competition experiments we find that a strain capable of autoregulation rapidly outcompetes a strain with a 5’ UTR mutation that cannot autoregulate, suggesting autoregulation promotes optimal cellular fitness.
The potential of exoskeletons in construction: barriers and challenges
Authors: Lindhard Søren Munch , Lassen Astrid Heidemann , Cheng Yang , Musso Matteo , Wang Geng , Bai Shaoping 
Published: December 2023

Purpose
Exoskeletons are moving into industries with the potential to reduce muscle strains and prevent occupational injuries. Although exoskeletons have been designed and tested in laboratory settings, rare empirical studies of their application in construction have been reported. Therefore, the purpose of this study is on in a real-life setting testing the applicability of adopting exoskeletons in the construction industry.

Design/methodology/approach
A feasibility study of exoskeletons in construction is conducted by testing a passive exoskeleton, designed for shoulder support. Five bricklayers tested in a two-month period the exoskeleton, each wearing it for a three-day period while carrying out normal work activities. Test data in terms of interviews were collected and analyzed using qualitative content analysis.

Findings
The application of exoskeletons in construction revealed several limitations, where the two primary ones are the exoskeleton is not designed while considering the tasks of a bricklayer causing several challenges and the exoskeleton only supports a single upward motion while limiting other movements and even counteracted when a downward movement was necessary.

Originality/value
The identified challenges could easily have been revealed by coupling the design and testing of exoskeletons to actual application. Thus, the design approach needs to be reversed. Instead of designing an exoskeleton to support a specific body part or motion and then identifying where it is applicable, it should target specific industries and focus on the actual work and movements and the necessary support. As part of the change, the design metrics should be reevaluated to reflect the work to support.
How to predict the entry mode of international construction contractor: a case-based reasoning approach
Authors: Li Huiling , Yuan Wenya , Xu Jianzhong 
Published: December 2023

PurposeThis study aimed to identify a specific taxonomy of entry modes for international construction contractors and to develop a decision-making mechanism based on case-based reasoning (CBR) to facilitate the selection of the most suitable entry modes.Design/methodology/approachAccording to the experience orientation of the construction industry, a CBR entry mode decision model was established, and based on successful historical cases, a two-step refinement process was carried out to identify similar situations. Then the validity of the model is proved by case analysis.FindingsThis study identified an entry mode taxonomy for international construction contractors (ICCs) and explored their decision-making mechanisms. First, a two-dimension model of entry mode for ICCs was constructed from ownership and value chain dimensions; seven common ICC entry modes were identified and ranked according to market commitment. Secondly, this study reveals the impact mechanism of the ICC entry mode from two aspects: the external environment and enterprise characteristics. Accordingly, an entry mode decision model is established.Practical implicationsFirstly, sorting out the categories of entry mode in the construction field, which provide an entry mode list for ICCs to select. Secondly, revealing the impact mechanism of ICC entry mode, which proposes a systematic decision-making system for the selection of ICC entry mode. Thirdly, constructing a CBR entry mode decision-making model from an empirical perspective, which offers tool support and reduces transaction costs in the decision-making process.Originality/valueThe study on entry modes for ICCs is still in the preliminary exploratory stage. The authors investigate the entry mode categories and decision-making mechanisms for ICCs based on Uppsala internationalization process theory. It widens the applied scope of Uppsala and promotes cross-disciplinary integration. In addition, the authors creatively propose a two-stage retrieval mechanism in the CBR model, which considers the order of decision variables. It refines the influence path of the decision variables on ICCs' entry mode.
A framework to integrate virtual reality into international standard safety trainings
Authors: Abotaleb Ibrahim S. , Elhakim Yasmin , El Rifaee Mohamed , Bader Sahar , Hosny Osama , Abodonya Ahmed , Ibrahim Salma , Sherif Mohamed , Sorour Abdelrahman , Soliman Mennatallah 
Published: December 2023

PurposeThe objective of this research is to propose an immersive framework that integrates virtual reality (VR) technology with directives international safety training certification bodies to enhance construction safety training, which eventually leads to safer construction sites.Design/methodology/approachThe adopted methodology combines expert insights and experimentation to maximize the effectiveness of construction safety training. The first step was identifying key considerations for VR models such as motion sickness prevention and adult learning theories. The second step was developing a game-like VR model for safety training, with multiple hazards and scenarios based on the considerations of the previous step. After that, safety experts evaluated the model and provided valuable feedback on its alignment with international safety training practices. Finally, the developed model is tested by senior students, where the testing format followed the Institution of Occupational Safety and Health (IOSH) working safely exam structure.FindingsAn advanced immersive VR safety training model was developed based on extensive lessons learned from the literature, previous work and psychology-informed adult learning theories. Model testing – through focus groups and hands-on experimentation – demonstrated significant benefit of VR in upgrading and complementing traditional training methods.Originality/valueThe findings presented in this paper make a significant contribution to the field of safety training within the construction industry and the broader context of immersive learning experiences. It also fosters further exploration into immersive learning experiences across educational and professional contexts.
Barriers to collaboration between school teachers and child care workers: implications for HRM and school leadership
Authors: Verheijen-Tiemstra Rachel , Ros Anje , Vermeulen Marc , Poell Rob F. 
Published: December 2023

PurposeWhilst an urgent need for collaboration is increasingly seen in education to better respond to socio-educational challenges, in practice, collaboration between primary school teachers and their partners is hampered by barriers. The aim of this study is to shed light on these barriers from a human resource management (HRM) angle, using the ability, motivation and opportunity (AMO) framework.Design/methodology/approachQuantitative and qualitative data were collected amongst staff in 16 child centres offering joint pre-school, education and childcare.FindingsThe authors' findings suggest that in general, both teachers and childcare workers perceive themselves as skilled and motivated for collaboration. They perceive aspects of opportunity to perform as most important barriers.Practical implicationsBased on this research, school leaders are advised to organise opportunities for collaboration, especially by fostering an inclusive organisational climate and scheduling sufficient time for collaboration.Originality/valueThis paper contributes to the relatively scarce body of research on HRM within the education sector. Furthermore, it illustrates the applicability of the AMO model for gaining insight into how educational management can be utilised to foster increased collaboration between teachers and childcare workers.
Education in collaboration with cities: the intentions of transdisciplinary courses
Authors: Bohm Nina Lotte , Klaassen Renate G. , van Bueren Ellen , den Brok Perry 
Published: December 2023

Purpose
In collaboration with their home cities, universities increasingly develop courses in which students investigate urban sustainability challenges. This paper aims to understand how far-reaching the collaboration with urban stakeholders in these courses is and what students are meant to learn from the transdisciplinary pedagogies.

Design/methodology/approach
This research is designed as a qualitative multiple-case study into the intentions of transdisciplinary courses in which universities collaborate with their home cities: Delft University of Technology in Delft and Amsterdam Institute for Advanced Metropolitan Solutions in Amsterdam. The study compares the written intentions of eight courses in course descriptions with the ideal intentions that teachers describe in interviews.

Findings
First, seven of the eight investigated courses were designed for urban stakeholders to participate at a distance or as a client but rarely was a course intended to lead to a collaborative partnership between the city and students. Second, the metacognitive learning objectives, such as learning to deal with biases and values of others or getting to know one’s strengths and weaknesses in collaboration, were often absent in the course descriptions. Learning objectives relating to metacognition are at the heart of transdisciplinary work, yet when they remain implicit in the learning objectives, they are difficult to teach.

Originality/value
This paper presents insight into the levels of participation intended in transdisciplinary courses. Furthermore, it shows the (mis)alignment between intended learning objectives in course descriptions and teachers’ ideals. Understanding both the current state of transdisciplinarity in sustainability courses and what teachers envision is vital for the next steps in the development of transdisciplinary education.
Do information disputes work: the effect of perceived risk, news disputes and credibility on consumer attitudes and trust toward biotechnology companies
Authors: Overton Holly K. , Yang Fan 
Published: December 2023

PurposeThis study examines a controversial issue (biotechnology) and how news disputes about misinformation related to the issue impacts individuals' attitudes toward a biotechnology company and their trust in the media source.Design/methodology/approachThis study conducts a 2 (risk: low vs. high) x 2 (pre-existing attitude: anti gene-editing technology vs. pro gene-editing technology) x 2 (dispute message: absent vs. present) x 2 (media source: Buzzfeed vs NYT) factorial online experiment using a Qualtrics panel (N = 1,080) to examine the impact on individuals' attitudes toward a biotechnology company and trust in the media source.FindingsResults indicate that dispute messages enhance attitudes toward the company but decrease trust in media sources. Interaction effects between pre-existing attitude and the dispute message, along with perceived risk and the dispute message, illustrate stark differences in how individuals with favorable vs. unfavorable pre-existing attitudes assessed the company after viewing the dispute message.Originality/valueThis study applies arguments from extant literature about prebunking and debunking misinformation. Specifically, this study investigates how dispute messages, a form of debunking through source derogation, actually impact individuals' perceptions of media credibility and/or their attitudes about the content they are reading. The study findings also reveal new insights regarding the interaction between pre-existing attitudes and perceived risk, as well as how dispute messages interact with each of the aforementioned factors.
The mediating role of corporate social responsibility in good corporate governance and firm value relationship: evidence from European financial institutions
Authors: Ben Fatma Hanen , Chouaibi Jamel 
Published: December 2023

Purpose
This paper aims to investigate the direct and indirect links between good corporate governance (GCG) and firm value using corporate social responsibility (CSR) as a mediating variable.

Design/methodology/approach
The data used in this research was collected from the Thomson Reuters Eikon ASSET4 database, involving 108 financial institutions belonging to 12 European countries listed on the stock exchange between 2007 and 2019. A multivariate linear regression analysis was conducted to test the hypotheses of this study.

Findings
Our results show that GCG has a positive effect on the firm value and CSR practices. Interestingly, the results indicate that CSR positively influences firm value. The results also reveal that CSR partially mediates the relationship between GCG and firm value.

Originality/value
This study contributes to the literature by providing evidence on how GCG increases firm value with the mediation mechanism of CSR in the link between GCG and firm value. To the best of our knowledge, it is the first research work documenting that GCG leads to better CSR, which ultimately results in increasing firm value of companies from the financial sector by bridging the information gap for this critical industry in the context of a developed market like Europe.
Combination of streamers' product description and viewers' comments: moderating effect of streamer–viewer relationship strength
Authors: Peng Yuhong , Ding Jianwei , Zhang Yueyan 
Published: December 2023

PurposeThis study examines the relationship between streamers' product descriptions, customer comments and online sales and focuses on the moderating effect of streamer–viewer relationship strength.Design/methodology/approachBetween June 2021 and April 2022, the structured data of 965 livestreaming and unstructured text data of 42,956,147 characters from two major live-streaming platforms were collected for the study. Text analysis and regression analysis methods were employed for data analysis.FindingsFirst, the authors' analysis reveals an inverted U-shaped relationship between comment length and product sales. Notably, comment volume and comment emotion positively influence product sales. Furthermore, the semantic richness, emotion and readability of streamers' product descriptions also positively influence product sales. Secondly, the authors find that the strength of streamer–viewer relationship weakens the positive effects of comment volume and comment emotion without moderating the inverted U-shaped effect of comment length. Lastly, the strength of streamer–viewer relationship also diminishes the positive effects of emotion, semantics and readability of streamers' product descriptions on product sales.Originality/valueThis study is the first to concurrently examine the direct and interactive effects of user-generated content (UGC) and marketer-generated content (MGC) on consumer purchase behaviors in livestreaming e-commerce, offering a novel perspective on individual decision-making and cue utilization in the social retail context.
The key to human performance: elevating wellbeing by bridging skills with meaningful work
Authors: Miller Andrea J. 
Published: December 2023

Purpose
This paper aims to explore the pivotal role of human resources (HR) in fostering employee engagement and well-being in a workplace transformed by digitalization. It addresses how HR can integrate meaningful work with enhanced well-being to create a more fulfilling and productive workplace.

Design/methodology/approach
The analysis is grounded in a synthesis of recent surveys, academic research and thought leadership in HR, providing a strategic framework for enhancing employee engagement in the digital age.

Findings
The research reveals that employees crave not only professional growth but also meaningful work and genuine connections, which are integral to their well-being. It suggests that HR strategies should align employee roles with organizational purpose and personal values and use technology to break down barriers to collaboration to increase trust and purpose-driven culture.

Originality/value
By combining insights from various fields, this paper contributes a unique perspective on the transformation of HR into a strategic force that aligns business objectives with employee well-being and the need for a purposeful work life.
‘Our therapeutic direction is towards Light’: transcendence and a non‐secular politics of difference in Islamic Counselling training
Authors: Dharamsi Sabnum , Liberatore Giulia 
Published: December 2023

AbstractThe Islamic Counselling training model discussed in this article first emerged in 1990s multicultural Britain within the newly expanding field of cross‐cultural counselling and psychotherapy. It is informed by classical Sufi notions of the self, the development of an Islamic psychology, and decolonial scholarship. Based on ethnographic research on the current training in Islamic Counselling, this article explores the ways in which this model trains students to engage with relational differences through non‐secular notions of reality, the self, and its relation to multiple others. Differences are made sense of through notions of ‘worldview’ and ‘journey’ that go beyond categories of culture, religion, and race, and while these differences are similarly ‘acknowledged’, there is also the possibility of surpassing them through an experiential process of ‘witnessing’. Islamic Counselling's therapeutic goal, therefore, is not the forging of a pious self, but transcendence: the establishing of a deeply felt understanding of Oneness or Truth. Through this process, Islamic Counselling holds difference and connection in view simultaneously, while challenging a relativist notion of cultural difference inherent in ethno‐psychiatry and cross‐cultural counselling. It offers a radically new mode of relating with differences within and beyond the counselling setting.