bluuebunny/crossref_metadata_2025_split huggingface dataset:
Composition of the essential oil of Pallenis spinosa (L.) Cass. (Asteraceae)
Authors: Senatore Felice , Bruno Maurizio 
Published: June 2003

AbstractThe essential oil of Pallenis spinosa (L.) Cass. was obtained by hydrodistillation. In total 38 components were identiﬁed by GC and GC–MS. Oxygenated sesquiterpenoids amounted to 60.2% of the oil. The main components were germacra‐1(10),5‐dien‐3,4‐diol (18.4%), α‐cadinol (14.1%), 3‐acetoxygermacra‐1(10),5‐dien‐4‐ol (13.0%), T‐cadinol (8.2%) and δ‐cadinene (5.8%). The oil does not show antimicrobial activity. Copyright © 2003 John Wiley & Sons, Ltd.
Composition and antibacterial activity of the essential oil of Anisochilus carnosus (Linn. ﬁl.) Benth., a Tamil plant acclimatized in Sicily
Authors: Senatore Felice , Lentini Francesca , Venza Francesca , Bruno Maurizio , Napolitano Francesco 
Published: June 2003

AbstractThe essential oil from aerial parts of Anisochilus carnosus (Linn. ﬁl.) Benth. (Lamiaceae) was obtained by hydrodistillation. The oil content was 0.13% (v/w), on a fresh weight basis. The oil composition was analysed by GC and GC–MS and 28 compounds were identiﬁed, accounting for 94.3% of the oil. Carvacrol (27.9%), camphor (14.1%) and α‐cis‐bergamotene (10.2%) were the most abundant components. The oil is active against Gram‐positive and Gram‐negative bacteria. Copyright © 2003 John Wiley & Sons, Ltd.
Chemical composition of Nepeta crassifolia Boiss. & Buhse oil from Iran
Authors: Dabiri M. , Seﬁdkon F. 
Published: June 2003

AbstractHydrodistillation of volatile oil from the aerial parts at full ﬂowering stage of Nepeta crassifolia Boiss. & Buhse was analysed by a combination of GC and GC–MS. Twenty‐one components were characterized in the oil layer. The main constituents were 4aα,7α,7aα‐nepetalactone (92.6%), linalyl acetate (2.6%), piperitone (0.80%), germacrene D (0.73%), 4aβ,7α,7aβ‐nepetalactone (0.64%) and β‐caryophyllene (0.58%). Copyright © 2003 John Wiley & Sons, Ltd.
Components of the essential oil from Petasites japonicus
Authors: Miyazawa Mitsuo , Teranishi Ayako , Ishikawa Yukio 
Published: June 2003

AbstractThe components of the essential oil from the leaves, roots and ﬂower stems of Petasites japonicus were analysed by GC and GC–MS. As a result, 69 compounds, amounting to 84–91% of the total oil, were characterized. The major components of the leaf essential oil were β‐caryophyllene (22%) and valencene (15%). The root oil contained angelic acid (18%), eremophilene (10%) and α‐phellandrene (10%) as the major components. On the ﬂower stem oil, valencene (12%), α‐phellandrene (11%) were the main components. Copyright © 2003 John Wiley & Sons, Ltd.
Essential oils of the leaves and stems of four Psidium spp.
Authors: da Silva João Domingos , Luz Arnaldo Iran R. , da Silva Milton Helio L. , Andrade Eloisa Helena A. , Zoghbi Maria das Graças B. , Maia José Guilherme S. 
Published: June 2003

AbstractThe essential oils of the leaves and ﬁne stems of Psidium acutangulum, P. striatulum, P. guineense and P. guajava were obtained by hydrodistillation and analysed by GC–MS. The main compounds identiﬁed in the oil of P. acutangulum were α‐pinene (14.8%), 1,8‐cineole (12.9%) and β‐pinene (10.1%). The oil of P. striatulum was dominated by β‐caryophyllene (28.6%), α‐selinene (7.7%), caryophyllene oxide (7.6%) and β‐selinene (7.4%). The major constituents found in the oil of P. guineense were β‐bisabolol (17.4%), limonene (6.8%) and epi‐α‐bisabolol (6.7%). The oil of P. guajava was dominated by α‐pinene (23.9%), 1,8‐cineole (21.4%) and β‐bisabolol (9.2%). Copyright © 2003 John Wiley & Sons, Ltd.
The role of age of acquisition and language usage in early, high‐proficient bilinguals: An fMRI study during verbal fluency
Authors: Perani Daniela , Abutalebi Jubin , Paulesu Eraldo , Brambati Simona , Scifo Paola , Cappa Stefano F. , Fazio Ferruccio 
Published: June 2003

AbstractWe assessed the effects of age of acquisition and language exposure on the cerebral correlates of lexical retrieval in high‐proficient, early‐acquisition bilinguals. Functional MRI was used to study Spanish–Catalan bilinguals who acquired either Spanish or Catalan as a first language in the first years of life. Subjects were exposed to the second language at 3 years of age, and have used both languages in daily life since then. Subjects had a comparable level of proficiency in the comprehension of both languages. Lexical retrieval with the verbal fluency task resulted in the well‐established pattern of left hemispheric activation centered on the inferior frontal region. The effect of age of acquisition was assessed by dividing the subjects into two groups, on the basis of the language acquired first (Catalan‐born or Spanish‐born bilinguals). Functional comparisons indicated that less extensive brain activation was associated with lexical retrieval in the language acquired earlier in life. The two groups were also different in language usage/exposure, as assessed with a specific questionnaire; in particular, the exposure to the second language (Spanish) was less intensive in the case of Catalans. This was reflected in a significant interaction, indicating a more extensive activation in Catalans during production in Spanish. Overall, these results indicate that, during a production task, both age of acquisition and language exposure affect the pattern of brain activation in bilinguals, even if both languages are acquired early and with a comparable level of proficiency. Hum. Brain Mapping 19:170–182, 2003. © 2003 Wiley‐Liss, Inc.
Androgen regulation of FLICE‐like inhibitory protein gene expression in the rat prostate
Authors: Nastiuk Kent L. , Kim John W. , Mann Mana , Krolewski John J. 
Published: June 2003

AbstractIn hope of eventually identifying defects in human prostatic neoplasias that render them insensitive to anti‐androgen therapy, we have examined the regulation of components of ligand‐induced cell death pathways during castration‐induced regression of the prostate. Rat prostates were obtained after surgical castration with or without subsequent androgen replacement. The mRNA levels of genes encoding components of the apoptotic pathway were measured from individual prostates. Whole prostates 1–10 days after castration did not show a significant change in mRNA levels encoding either Fas or FasL, which some studies suggest are necessary for regression to occur. However, the mRNA encoding a catalytically inactive cysteinyl aspartate‐specific protease (caspase) analog, FLICE‐like inhibitor protein (FLIP), decreases during the first day following castration. In the most apoptotically responsive ventral lobe of the rat prostate, the reduction in FLIP mRNA levels is evident within 12 h of castration. The mRNA levels of the principal target of FLIP inhibition, caspase‐8, do not change during the period preceding the onset of detectable DNA fragmentation. Androgen administration to castrated rats reverses prostate regression, and restores FLIP mRNA to normal levels. By acting as an inhibitor of caspase‐8, FLIP may protect prostatic epithelium from apoptosis. Androgen withdrawal, by reducing FLIP mRNA levels, might leave the cells vulnerable to as yet unidentified cell death signals. J. Cell. Physiol. 196: 386–393, 2003. © 2003 Wiley‐Liss, Inc.
Role of cell‐cycle regulatory proteins in gynecological cancer
Authors: Milde‐Langosch Karin , Riethdorf Sabine 
Published: June 2003

AbstractHuman malignant tumors are characterized by abnormal proliferation resulting from alterations in cell‐cycle regulatory mechanisms. This review summarizes the current knowledge about these aberrations in malignant tumors of the ovary, endometrium, cervix uteri, and vulva. The data indicate that analysis of single cell cycle stimulating or inhibiting proteins partly produces unexpected, apparently paradoxical results, and cell‐cycle regulatory pathways should be regarded as a whole in order to identify the molecular mechanisms leading to abnormal tumor cell proliferation. For the papillomavirus (HPV)‐ associated cervical and vulvar carcinomas, the manifold effects of the viral oncogenes E6 and E7 on cell‐cycle control are described. J. Cell. Physiol. 196: 224–244, 2003. © 2003 Wiley‐Liss, Inc.
Caffeine induces G2/M arrest and apoptosis via a novel p53‐dependent pathway in NB4 promyelocytic leukemia cells
Authors: Ito Keisuke , Nakazato Tomonori , Miyakawa Yoshitaka , Yamato Kenji , Ikeda Yasuo , Kizaki Masahiro 
Published: June 2003

AbstractMethylxantine derivative, caffeine, is known to prevent the p53‐dependent apoptosis pathway via inhibition of ATM (ataxia telangiectasia mutated) kinase, which activates p53 by phosphorylation of the Ser‐15 residue. In contrast, it has been reported that caffeine induces p53‐mediated apoptosis through Bax protein in non‐small‐cell lung cancer cells. Therefore, the effects of caffeine on cellular growth in malignant cells are controversial. We investigated the effects of caffeine on cell proliferation, cell cycle progression, and induction of apoptosis in NB4 promyelocytic leukemia cells containing wild‐type p53. Caffeine suppressed the cellular growth of NB4 cells in a dose‐ and time‐dependent manner. Caffeine induced G2/M phase cell cycle arrest in NB4 cells in association with the induction of phosphorylation at the Ser‐15 residue of p53 and induction of tyrosine phosphorylation of cdc2. Expression of Bax protein was increased in NB4 cells after treatment with caffeine. Interestingly, the antisense oligonucleotides for p53 significantly reduced p53 expression and caffeine‐induced G2/M phase cell cycle arrest in NB4 cells. These results suggest that caffeine induces cell cycle arrest and apoptosis in association with activation of p53 by a novel pathway to phosphorylate the Ser‐15 residue and induction of phosphorylation of cdc 2 in leukemic cells with normal p53. © 2003 Wiley‐Liss, Inc.
Eicosapentaenoic acid inhibits PDGF‐induced mitogenesis and cyclin D1 expression via TGF‐β in mesangial cells
Authors: Hida Mariko , Fujita Hisayo , Ishikura Kenji , Omori Sayu , Hoshiya Makiko , Awazu Midori 
Published: June 2003

AbstractEicosapentaenoic acid (EPA), an ω‐3 polyunsaturated fatty acid derived from fish oil, is efficacious in glomerular diseases where mesangial proliferation is a key event. We examined the mechanisms of action of EPA on platelet‐derived growth factor (PDGF)‐stimulated rat mesangial cell mitogenesis. EPA dose‐dependently inhibited PDGF‐stimulated [3H]‐thymidine incorporation. PDGF‐induced PDGF receptor autophosphorylation, an initial event for PDGF signaling, was not affected by 2 µg/ml EPA. Similarly, PDGF‐stimulated activation of extracellular signal‐regulated kinase (ERK) was not altered. On the other hand, EPA inhibited cyclin‐dependent kinase 4 (CDK4) activation and cyclin D1 protein induction, a critical step for G1/S progression. TGF‐β secretion assessed by ELISA and bioassay was increased by EPA at 18 h. Coincubation with anti‐TGF‐β antibody inhibited the EPA‐induced suppression of [3H]‐thymidine incorporation and cyclin D1 expression. SB203580, an inhibitor of p38, a downstream kinase of TGF‐β, did not affect EPA's growth inhibitory effect. These results demonstrate that EPA inhibits PDGF‐stimulated mesangial cell mitogenesis and cyclin D1 expression via TGF‐β. J. Cell. Physiol. 196: 293–300, 2003. © 2003 Wiley‐Liss, Inc.
Geldanamycin inhibits migration of glioma cells in vitro: A potential role for hypoxia‐inducible factor (HIF‐1α) in glioma cell invasion
Authors: Zagzag David , Nomura Motohiro , Friedlander David R. , Blanco CY , Gagner Jean‐Pierre , Nomura Naoko , Newcomb Elizabeth W. 
Published: June 2003

AbstractFocal adhesion kinase (FAK) and hypoxia‐inducible factor (HIF‐1α) are both up‐regulated in glioblastoma multiforme (GBMs), particularly in invasive zones. Because FAK may play an important role in the invasion of glioma cells into the surrounding brain, we sought an agent that causes down‐regulation of FAK phosphorylation as a potential inhibitor of brain tumor invasion and growth. Geldanamycin (GA), a benzoquinone ansamycin antibiotic, binds to heat shock protein 90 (Hsp90) and interferes with its function. GA inhibits the proliferation of various non‐glial cells and has anti‐tumor activity. Moreover, GA blocks HIF‐regulated transcription of VEGF and inhibits the VEGF‐induced phosphorylation of FAK and migration of endothelial cells. Here, we tested the effect of GA on glioma cell migration in vitro and its potential to down‐regulate HIF‐1α induction. Our results demonstrate that GA (i) decreases U87MG, LN229, and U251MG glioma cell migration; (ii) reduces cell migration independent of p53 and PTEN status; (iii) prevents migration at non‐toxic concentrations; (iv) reduces phosphorylation of FAK; and (v) inhibits cobalt chloride (CoCl2)‐mediated induction of HIF‐1α in glioma cells. To the best of our knowledge, this is the first report showing that GA can inhibit phosphorylation of FAK concomitant with a decrease in cellular migration. One of the most clinically relevant aspects of this study is that GA interferes with the induction of HIF‐1α that has been linked with glioma cell migration and angiogenesis. Given the fact that GA is a small lipophilic molecule capable of penetrating the blood brain barrier together with the data presented here provide a strong rationale for its use or its analogues in the treatment of highly invasive GBMs. J. Cell. Physiol. 196: 394–402, 2003. © 2003 Wiley‐Liss, Inc.
Modulation of PAI‐1 and proMMP‐9 syntheses by soluble TNFα and its receptors during differentiation of the human monocytic HL‐60 cell line
Authors: Peiretti Franck , Bernot Denis , Lopez Sophie , Bonardo Bernadette , Deprez‐Beauclair Paule , Juhan‐Vague Irène , Nalbone Gilles 
Published: June 2003

AbstractDuring phorbol ester‐induced differentiation of HL‐60 monocytic cells, tumor necrosis factorα (TNFα) synthesis and secretion are increased, which contributes to the autocrine regulation of TNFα‐responsive genes. We investigated how, during phorbol ester‐induced differentiation of HL‐60 cells, the secreted TNFα modulated plasminogen activator inhibitor type I (PAI‐1) and gelatinase B (MMP‐9) syntheses, two proteins involved in pericellular proteolysis. The differentiation‐induced release of TNFα, was abolished by the hydroxamate‐based matrix metalloproteinase (MMP) inhibitor, RU36156. RU36156 or a neutralizing anti‐TNFα significantly down‐regulated PAI‐1 synthesis exclusively during the early phases of differentiation (from promyelocyte to monocytic‐like cells), which underlined the activating role of autocrine TNFα during this time range. As cells progressed to monocyte/macrophage phenotype, they still released TNFα, but RU36156 or anti‐TNFα no longer had an effect on PAI‐1 synthesis. This lack of effect was not due to a default of TNFα signaling since PAI‐1 synthesis was still stimulated in response to exogenous TNFα. TNFα receptor RI was also actively released and was shown to reduce TNFα activity which may account for the inability of soluble TNFα to up‐regulate PAI‐1 synthesis. In later mature stage, cells became susceptible to exogenous TNFα‐induced apoptosis and rapidly lost their ability to respond to TNFα. The MMP‐9 synthesis followed similar regulation as PAI‐1. Isolated human blood monocytes‐derived macrophages behave like HL‐60‐derived macrophages. In conclusion, these results show that during leukocyte differentiation, time windows exist during which the autocrine TNFα is active and then down‐regulated by RI, which may temper a continuous up‐regulation of the synthesis of proteins involved in pericellular proteolysis. J. Cell. Physiol. 196: 346–353, 2003. © 2003 Wiley‐Liss, Inc.
Mda‐7/IL‐24 induces apoptosis of diverse cancer cell lines through JAK/STAT‐independent pathways
Authors: Sauane Moira , Gopalkrishnan Rahul V. , Lebedeva Irina , Mei Mei Xin , Sarkar Devanand , Su Zhao‐Zhong , Kang Dong‐Chul , Dent Paul , Pestka Sidney , Fisher Paul B. 
Published: June 2003

AbstractExperimental evidence documents that the MDA‐7/IL‐24 protein (an IL‐10 family cytokine) binds to IL‐20 and IL‐22 receptor complexes resulting in the activation of JAK/STAT signaling pathways. Recent published reports utilizing human blood derived primary lymphocytes have provided additional confirmatory evidence relating to the cytokine properties of this molecule. A notable attribute of mda‐7/IL‐24 is its cancer cell‐specific apoptosis inducing capacity, which currently remains incompletely understood. Treatment with distinctive tyrosine kinase inhibitors (Genistein and AG18) or a JAK‐selective inhibitor (AG490) did not prevent Ad.mda‐7 induced apoptosis in diverse cell lines. In addition, there is no apparent correlation between patterns of expression of IL‐20R1, IL‐20R2, and IL‐22R mRNA and susceptibility to Ad.mda‐7 in different cell lines. Furthermore, Ad.mda‐7 is able to induce killing in STAT/JAK deficient cells. In contrast, treatment with the p38MAPK selective inhibitor SB203580, partially inhibited apoptosis induced by Ad.mda‐7 in different cell lines. These results demonstrate for the first time that signaling events leading to susceptibility to Ad.mda‐7 induced apoptosis, might be tyrosine kinase independent and can thus be distinguished from its cytokine function related properties mediated by the IL‐20/IL‐22 receptor complexes that require JAK/STAT kinase activity. J. Cell. Physiol. 196: 334–345, 2003. © 2003 Wiley‐Liss, Inc.
Eyelid movements in normal human fetuses
Authors: Petrikovsky Boris M. , Kaplan Gary , Holsten Nancy 
Published: June 2003

AbstractPurposeWe undertook this study to investigate the characteristics of blinking activity in healthy human fetuses.MethodsBlinking activity was studied sonographically in healthy fetuses between 33 and 42 weeks' menstrual age. Horizontal and coronal sonographic views of the eye were obtained and videotaped. Fetal blinking movements were analyzed by reviewing the videotape in slow motion. Fetal vibroacoustic stimulation was also used when clinically indicated.ResultsWe examined a total of 18 healthy fetuses. Fetal blinking was detected in 89% of cases, with a mean frequency of 6.2 movements per 60‐minute observation period. Vibroacoustic stimulation was associated with increased fetal blinking in the 6 fetuses in which it was applied (mean, 15.3 movements per 60‐minute observation period).ConclusionsBlinking is a normal fetal activity. The increased frequency of blinking activity associated with vibroacoustic stimulation may be considered a part of the normal startle reflex. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:299–301, 2003
Acute increase in intracranial pressure revealed by transcranial Doppler sonography
Authors: Reinhard Matthias , Petrick Manfred , Steinfurth Georg , Ziyeh Sargon , Hetzel Andreas 
Published: June 2003

AbstractIncreased pulsatility of blood flow in the basal cerebral arteries recorded with the use of transcranial Doppler sonography (TCD) corresponds to increased intracranial pressure (ICP) to near the level of the diastolic arterial blood pressure. We describe the case of a 39‐year‐old woman who developed acute reduction in consciousness, anisocoria, gaze deviation, and right‐sided hemiparesis 2 days after resection of a relapsed astrocytoma. MRI revealed only a moderate enlargement of the ventricles, but TCD revealed highly pulsatile waveforms of all basal cerebral arteries, showing a biphasic pattern with diastolic backflow. This pattern was interpreted as a massive increase in ICP with imminent danger of cerebral circulatory arrest corresponding to acute malresorptive hydrocephalus. External cerebrospinal fluid drainage was immediately undertaken, revealing excessive ICP of more than 50 cm H2O. Twenty‐four hours after this intervention, both the ICP and the Doppler waveforms had returned to normal. This case illustrates the usefulness of TCD for diagnosing a critical but potentially reversible acute increase in ICP with imminent cerebral circulatory arrest. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:324–327, 2003
Visualization of bilateral carotid cavernous sinus fistulas with duplex sonography
Authors: Sanden Ulrike , Grosse Uwe , Jaksche Hans 
Published: June 2003

AbstractDuplex sonography used as a primary diagnostic tool in the case of a 17‐year‐old boy with a traumatic head injury revealed bilateral carotid cavernous sinus fistulas, with bilateral dilated venous convolutions next to the carotid siphon and dilated superior ophthalmic veins. A bilateral craniectomy allowed visualization of the entire circle of Willis together with the dilated cavernous sinuses. Doppler spectral analysis of blood flow in the arterialized superior ophthalmic veins revealed an arterialized venous pattern with retrograde and increased blood flow. The same blood flow profile was found in the venous cavernous sinuses. These findings were confirmed by digital subtraction angiography. We planned to perform embolization of the patient's fistulas, but intracranial and subarachnoid hemorrhaging developed, and the patient died the day before the procedure was to have been performed. The entire pathologic state of carotid cavernous sinus fistulas, from their origin beside the carotid siphon to the superior ophthalmic veins, can be visualized with duplex sonography, particularly when patients have undergone craniectomy. We believe that patients with frontal or basilar skull fracture should undergo duplex sonographic examination to detect carotid cavernous sinus fistulas. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:319–323, 2003
Sonographic detection of multifocality in breast carcinoma
Authors: Park Jeong Mi , Yoon Ghil‐Suk , Kim Sun Mi , Ahn Sei‐Hyun 
Published: June 2003

AbstractPurposeThe aim of this study was to evaluate the diagnostic accuracy and clinical value of preoperative sonographic detection of multifocality in breast carcinoma.MethodsBetween November 1, 1999, and November 30, 2000, we prospectively compared the sonographic and histopathologic findings for all nodules measuring 1 cm or less in the longest dimension on sonography in 19 consecutive patients diagnosed with breast carcinoma. For each nodule, the final histopathologic diagnosis, size, and, in cases of nodules in the same breast as the primary lesion, the distance from that lesion were evaluated.ResultsThe 19 patients examined, all of whom were women, had a mean age of 42.1 years (range, 28–62 years). Thirty nodules were detected on sonography: 15 patients had a total of 24 nodules in the ipsilateral breast, 2 patients each had 1 nodule in the contralateral breast, and 2 patients each had 1 nodule in the ipsilateral breast and 1 nodule in the contralateral breast, for a total of 26 ipsilateral and 4 contralateral nodules. Of the 30 lesions, 20 (67%) appeared to be malignant (ie, satellite nodules) on sonography. Histopathologic examination showed that of these malignant‐looking lesions, 15 (75%), which were found in 10 patients, were malignant and 5 (25%), which were found in 4 patients, were benign. For 7 (70%) of the 10 patients whose nodules appeared malignant on sonography, the initial treatment plan was changed because of the sonographic findings. Ten lesions were assessed as benign on sonography; histopathologic examination confirmed that all 10 were benign. Sonography had a sensitivity of 100%, a specificity of 67%, a positive predictive value of 75%, a negative predictive value of 100%, and an accuracy rate of 83%. The mean size of the nodules was 0.8 cm (range, 0.3–1.4 cm). The mean distance of the 26 ipsilateral nodules from the primary lesion was 1.8 cm (range, 0.5–6 cm).ConclusionsPreoperative sonography is accurate and reliable for detecting small satellite nodules and can have an important role in the preoperative evaluation of patients for whom breast‐conserving surgery is planned. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:293–298, 2003