bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for thromboembolic events in patients hospitalized for COVID-19 pneumonia in a general ward and requiring treatment with oxygen
Authors: Degrave Raphaël , Murris Juliette , Charles-Nelson Anaïs , Hermine Olivier , Porcher Raphaël , Ravaud Philippe , Mariette Xavier , Tharaux Pierre-Louis , Resche-Rigon Matthieu , Sanchez Olivier , Katsahian Sandrine ,   The CORIMUNO-19 Collaborative Group, Arlet Jean-Benoît 
Published: November 2023

Abstract

Purpose
To assess risk factors for arterial and venous thromboses (AVT) in patients hospitalized in general wards for COVID-19 pneumonia and requiring oxygen therapy.

Methods
Our study was based on three randomized studies conducted as part of the CORIMUNO-19 platform in France between 27 March and 26 April 2020. Adult inpatients with COVID-19 pneumonia requiring at least 3 l/min of oxygen but not ventilation were randomized to receive standard care alone or standard care plus biologics. Patients were followed up for 3 months, and adverse events were documented. Risk factor for AVT and bleeding was identified by analyzing clinical, laboratory, and treatment data at baseline among the 315 patients with complete datasets. A Fine and Gray model was used to take account of competing events.

Results
During the 3-month follow-up period, 39 AVT occurred in 38 (10%) of the 388 patients: 26 deep vein thromboses and/or pulmonary embolisms in 25 (6%) patients, and 14 arterial thrombotic events in 13 (3%) patients. A history of diabetes at inclusion [sHR (95% CI) = 2.65 (1.19–5.91), P = .017] and the C-reactive protein (CRP) level (sHR = 1 [1–1.01], P = .049) were significantly associated with an elevated risk of thrombosis. Obesity was not associated with a higher risk of thrombosis (sHR = 1.01 [0.4–2.57], P = .98). The CRP level and diabetes were not risk factors for hemorrhage.

Conclusion
Among patients hospitalized in general wards for COVID-19 pneumonia during the first wave of the epidemic, diabetes (but not obesity) and a high CRP level were risk factors for AVT. The use of higher doses of anticoagulant in these high-risk patients could be considered.
Bioactive citrate-based polyurethane tissue adhesive for fast sealing and promoted wound healing
Authors: Li Yan , Liu Jiawei , Lian Chenxi , Yang He , Zhang Mingjiang , Wang Youfa , Dai Honglian 
Published: November 2023

Abstract
As a superior alternative to sutures, tissue adhesives have been developed significantly in recent years. However, existing tissue adhesives struggle to form fast and stable adhesion between tissue interfaces, bond weakly in wet environments and lack bioactivity. In this study, a degradable and bioactive citrate-based polyurethane adhesive is constructed to achieve rapid and strong tissue adhesion. The hydrophobic layer was created with polycaprolactone to overcome the bonding failure between tissue and adhesion layer in wet environments, which can effectively improve the wet bonding strength. This citrate-based polyurethane adhesive provides rapid, non-invasive, liquid-tight and seamless closure of skin incisions, overcoming the limitations of sutures and commercial tissue adhesives. In addition, it exhibits biocompatibility, biodegradability and hemostatic properties. The degradation product citrate could promote the process of angiogenesis and accelerate wound healing. This study provides a novel approach to the development of a fast-adhering wet tissue adhesive and provides a valuable contribution to the development of polyurethane-based tissue adhesives.
Governance around family wealth: How to preserve a long-term value for families?
Authors: Gruna Mirek , Ronovská Kateřina 
Published: November 2023

Abstract
Preservation of long-term values has been a challenge for wealthy families over centuries. What is valuable to each family should not only be measured in financial terms but also in the context of family harmony, avoiding disputes, and ensuring a shared understanding of the family’s long-term goals. This article focuses on and explains some key mechanisms that can assist families in the preservation and enhancement of those long-term values and goals, from informal ones such as family mission statements to more formal arrangements like family charters. The authors offer jurisdictional comparison of examples as to how these approaches apply in practice, including discussion about the legal enforceability of family charters.
A 6-yr evaluation of prescribed-fire timing on yearling cattle growth performance and plant community dynamics on native tallgrass prairie in the Kansas Flint Hills
Authors: Duncan Z M , Tajchman A J , Lemmon J , Hollenbeck W R , Blasi D A , Fick W H , Olson K C 
Published: November 2023

Abstract
A 6-yr experiment was conducted to determine the effects of prescribed-fire season on stocker cattle growth performance and rangeland plant community characteristics in the Kansas Flint Hills. Eighteen pastures were grouped by watershed and each watershed was randomly assigned to 1 of 3 prescribed-fire treatments: spring (11 April ± 5.7 d), summer (25 August ± 6.2 d), or autumn (2 October ± 9.0 d). All burns were applied prior to grazing in years 1, 2, 3, and 5; however, no burns were applied in year 4 because of unfavorable burn conditions. Over 5 consecutive grazing seasons, 1,939 yearling stocker calves (initial BW = 281 ± 58.9 kg) were grazed from May to August at a targeted stocking density of 280 kg live-weight + ha−1. Beginning in June of 2018 (pretreatment), a permanent 100-m transect was established in each pasture and was used to determine plant-species composition using a modified step-point method. Forage biomass accumulation and root carbohydrate concentrations of 4 native tallgrass plant species were also measured. All data were analyzed as a completely randomized design using a mixed model. Average daily gain (ADG) was 0.05 to 0.07 kg greater (P = 0.02) for calves grazing spring-burned pastures compared with calves grazing summer- or autumn-burned pastures; however, ADG did not differ (P ≥ 0.55) between calves assigned to the summer or autumn prescribed-fire treatments. Basal cover of all graminoids and all forbs did not differ (P ≥ 0.30) among prescribed-fire treatments; however, basal cover of C3 grasses tended (P = 0.06) to be greater while basal cover of C4 grasses tended (P = 0.08) to be less in autumn-burned pastures compared with spring-burned pastures. Forage biomass accumulation did not differ (P = 0.58) among treatments. In addition, root starch or root water-soluble carbohydrate concentrations in big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), Indiangrass (Sorghastrum nutans), or purple prairieclover (Dalea purpurea) did not differ (P ≥ 0.26) among prescribed-fire treatments. Overall, we interpreted these data to suggest that prescribed-fire timing had small influences on yearling stocker cattle growth performance and rangeland plant composition but did not influence forage biomass accumulation or root carbohydrate concentrations of key native tallgrass plant species in the Kansas Flint Hills.
Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia
Authors: Dols-Serrate Daniel , Stocchino Giacinta Angela , Sluys Ronald , Riutort Marta 
Published: November 2023

Abstract
Dugesia hepta and D. benazzii are two species found in Sardinia and Corsica. Previous studies have been unable to resolve their evolutionary relationships and there was doubt about the monophyly of D. benazzii. This study used molecular and morphological data to develop a rigorous species-delimitation procedure within an integrative framework. Three different species discovery methods (ABGD, GMYC, and mPTP) were applied on single-locus data to formulate primary species hypotheses. Secondary species hypotheses were proposed based on two Bayesian species-validation methods (BPP and BFD), morphological, and karyological traits. This study presents evidence supporting the recognition of two new species: Dugesia mariae from Corsica and Dugesia hoidi from Sardinia. A redescription of D. benazzii is also reported. The phylogenetic relationships between these four species were resolved with high support, except for D. hoidi. The performance of the different molecular-delimitation methodologies is discussed, as well as the presence of ciliate parasites in D. benazzii and D. mariae.
Obesity Increases Risk for Wound Complications After Pediatric Foot Surgery: A Retrospective Cohort Review Using the NSQIP-Pediatric Database
Authors: Farahani Farzam , Rodriguez Joel A. , Wukich Dane K. , Zide Jacob R. , Riccio Anthony I. 
Published: November 2023

Background:
As the incidence of childhood obesity continues to rise, so too does the number of obese children who undergo foot surgery. As the childhood obesity epidemic rolls on, pediatric orthopaedic surgeons will encounter obese patients with even greater frequency. Therefore, a comprehensive understanding of the risks associated with obesity is valuable to maximize patient safety. The purpose of this study is to retrospectively evaluate the relationship between obesity and postoperative outcomes in patients undergoing pediatric foot surgery across multiple institutions using a large national database.

Methods:
Pediatric patients who had undergone foot surgery were retrospectively identified using the American College of Surgeons 2012-2017 Pediatric National Surgical Quality Improvement (ACS-NSQIP-Pediatric) database by cross-referencing reconstructive foot-specific CPT codes with ICD-9/ICD-10 diagnosis codes. Center for Disease Control BMI-to-age growth charts were used to stratify patients into normal-weight and obese cohorts. Univariate and multivariate analyses were performed to describe and assess outcomes in obese compared with normal-weight patients.

Results:
Of the 3924 patients identified, 1063 (27.1%) were obese. Compared with normal-weight patients, obese patients were more often male (64.7% vs. 58.7%; P=0.001) and taller (56.3 vs. 51.3 inches; P<0.001). Obese patients had significantly higher rates of overall postoperative complications (3.01% vs. 1.32%; P=0.001) and wound dehiscence (1.41% vs. 0.59%; P=0.039). Multivariate analysis found that obesity was an independent predictor of both wound dehiscence [adjusted odds ratio (OR)=2.16; 95% CI=1.05-4.50; P=0.037] and surgical site infection (adjusted OR=3.03; 95% CI=1.39-6.61; P=0.005). Subgroup analysis of patients undergoing clubfoot capsular release procedures identified that obese patients had a higher rate of wound dehiscence (3.39% vs. 0.51%; P=0.039) compared with normal-weight patients. In multivariate analysis, obesity was an independent predictor of dehiscence (adjusted OR=5.71; 95% CI=1.46-22.31; P=0.012) in this procedure group. There were no differences in complication rates between obese and normal-weight patients in a subgroup analysis of tarsal coalition procedures or clubfoot tibialis anterior tendon transfer procedures.

Conclusion:
Obese children undergoing foot surgery had higher overall complication rates, wound complications, and surgical site infections compared with children of normal weight. As the incidence of childhood obesity continues to rise, this information may be useful in assessing and discussing surgical risks with patients and their families.

Level of Evidence:
III.
Tel-Aviv Protocol for Postrefractive Surgery Corneal Ectasia: A Case Series
Authors: Masarwa Dua , Blockstein Keren , Sela Tzahi , Munzer Gur , Kaiserman Igor 
Published: November 2023

Purpose:
The aim of this study was to describe the safety and efficacy of the Tel-Aviv Protocol (epithelial photorefractive keratectomy and corneal cross-linking) as a treatment for postrefractive surgery corneal ectasia.

Methods:
This study includes 8 eyes from 7 patients, each diagnosed with postrefractive surgery ectasia years after refractive surgery and treated with the Tel-Aviv Protocol. The procedure included transepithelial PRK using the EX500 excimer laser, a 50-μm laser ablation of the epithelium and anterior stroma, delivered on the visual axis with cyclotorsion correction, treating up to 50% of refractive astigmatism. After the epithelial photorefractive keratectomy, all patients underwent corneal cross-linking.

Results:
The mean keratometry (K) and maximal K both decreased [from 43.37 ± 2.23 to 41.84 ± 2.01 (P = 0.03) and from 44.95 ± 3.08 to 42.78 ± 2.19 (P = 0.03), respectively]. Astigmatism was significantly reduced (from 3.53 ± 2.36 to 0.88 ± 0.89 diopter; P = 0.02). Uncorrected visual acuity improved significantly in all patients from a mean of 0.56 ± 0.32 to 0.15 ± 0.14 logMAR (P = 0.01). Best-corrected visual acuity improved from 0.22 ± 0.24 to 0.06 ± 0.06 logMAR (P = 0.07; 1-tail P = 0.04). All patients maintained visual acuity during the follow-up period, up to 25 months, mean = 329 days. The Tel-Aviv Protocol was found to be safe [mean safety index: 1.63 ± 1.03 (range: 1.00–4)] and effective [mean efficacy index: 1.29 ± 0.66 (range: 0.71–2.1)].

Conclusions:
The Tel-Aviv Protocol, developed initially for keratoconus treatment, is a safe and promising procedure to stop postrefractive surgery ectasia progression while significantly improving vision, thereby avoiding keratoplasty.
Velopharyngeal Insufficiency Following Furlow Versus Straight Line Repair With Intravelar Veloplasty: A Single-institution Experience
Authors: Davis Matthew J. , Roy Michelle G. , Burns Heather R. , Yim Nicholas , Wilson Kristina D. , Moore Ellen E. , Buchanan Edward P. , Monson Laura A. 
Published: November 2023

Background:
Measurements of postoperative velopharyngeal dysfunction (VPD) can be used to determine the efficacy of a palatoplasty operation. Hypernasality and audible nasal air emission are typical manifestations of VPD during speech. We aimed to longitudinally compare VPD outcomes in postpalatoplasty patients who underwent Furlow repair versus straight line repair with intravelar veloplasty (IVVP). Additionally, we examined the relationship between VPD outcomes and select pre-existing patient characteristics.

Methods:
Retrospective chart review was performed to identify primary palatoplasty patients treated from April 2012 to March 2021. Variables collected included gender, syndromic status, primary language, Veau cleft type, type of speech assessment, age at time of surgery, degree of hypernasality, presence of audible nasal air emission, and overall adequacy of velopharyngeal function. Pearson χ2 test and multivariable t tests were used to analyze variables. Logistic regression was used to control for statistically significant variables.

Results:
Of the 118 patients included, 38 received a Furlow procedure and 80 received a straight line with IVVP procedure. Audible nasal air emission was present in 57.3% of straight line with IVVP patients and 42.9% of Furlow patients, with no statistically significant difference between groups. Clinically significant hypernasality was present in 42.1% of straight line with IVVP patients and 22.9% of Furlow patients (P=0.05). Velopharyngeal function was classified as adequate in 63.5% of straight line with IVVP patients and 83.3% of Furlow patients (P=0.03). However, after stratifying by syndromic versus nonsyndromic status, there was no statistically significant difference between straight line with IVVP and Furlow patients for postoperative hypernasality and velopharyngeal function.

Conclusions:
This study suggests that there are no statistically significant differences between straight line with IVVP and Furlow palatoplasty techniques regarding speech outcomes including hypernasality, audible nasal air emission, and overall VP function. Furthermore, select patient characteristics such as gender, primary language, syndromic status, age at repair, and Veau cleft type do not significantly impact postoperative speech outcomes.
Surgical Treatment of Craniofacial Fibrous Dysplasia With TP53 Gene Mutation
Authors: Chen Lang , Chen YuJing , Xiao Kai , Hu Fei , Wang HuanMing , Shao Qiang 
Published: November 2023

Background:
To report the surgical treatment of craniofacial fibrous dysplasia (CFD) with TP53 gene mutation.

Methods:
The patient was diagnosed with CFD by surgery at the age of 14 years. At the age of 35 years, the tumor recurred, and the patient took active treatment. The tumor was resected 4 times by neuroendoscopy due to recurrence in a short period. Meanwhile, genetic tests were performed on the patient. The patient’s postoperative pathology indicated leiomyosarcoma and genetic testing indicated TP53 gene mutation.

Results:
Despite the active surgical treatment, the patient finally died of a malignant tumor. The prognosis of patients with CFD malignancy accompanied by TP53 gene mutation is poor, and its treatment is difficult.

Conclusions:
The prognostic benefit of surgical treatment for patients with CFD malignancy is limited. It is hoped that more genetic mutations will be identified and reported in patients with CFD malignancy, and long-term follow-up is necessary for patients with current fibrous dysplasia or CFD.
A decision-making tool to navigate through Extracellular Vesicle research and product development
Authors: Loria Francesca , Picciotto Sabrina , Adamo Giorgia , Zendrini Andrea , Raccosta Samuele , Manno Mauro , Bergese Paolo , Liguori Giovanna L. , Bongiovanni Antonella , Zarovni Nataša 
Published: November 2023

AbstractDue to their intercellular communication properties and their involvement in a wide range of biological processes, extracellular vesicles (EVs) are increasingly being studied and exploited for different applications. Nevertheless, their complex nature and heterogeneity, as well as the challenges related to their isolation, purification and characterization procedures, require cautious assessment of the quality and quantity parameters to monitor. This translates into a multitude of choices and putative solutions that lie in front of any EV researcher, in both research and translational environments, resembling a labyrinth with multiple paths to cross and, possibly, more than one exit. In this respect, decision-making tools might represent our modern Ariadne’s string to follow not to get lost or distracted along the journey, to choose the shorter and best-fit-to-source EV application(s) andvice versa. Here, we present the implementation of a multi-criteria EV decision-making grid (EV-DMG) as a novel, customizable, efficient and easy-to-use tool to support responsible EV research and innovation. By identifying and weighting key assessment criteria for comparing distinct EV-based preparations and/or processes, our EV-DMG may assist any EV community member in making informed, transparent and reproducible decisions regarding the EV sources and/or samples to be managed, as well as the most suitable production and/or analytical pipelines to be adopted for targeting a defined aim or application.
‘Drifting’Buchneragenomes track the microevolutionary trajectories of their aphid hosts
Authors: Thia Joshua A. , Zhan Dongwu , Robinson Katie , Umina Paul A. , Hoffmann Ary A. , Yang Qiong 
Published: November 2023

ABSTRACTEvolution ofBuchnera–aphid host symbioses is often studied among species at macroevolutionary scales. Investigations within species offer a different perspective about how eco-evolutionary processes shape patterns of genetic variation at microevolutionary scales. Our study leverages new and publicly available whole-genome sequencing data to studyBuchnera–aphid host evolution inMyzus persicae, the peach potato aphid, a globally invasive and polyphagous pest. Across 43 different asexual, clonally reproducing isofemale strains, we examined patterns of genomic covariation betweenBuchneraand their aphid host and considered the distribution of mutations in protein-coding regions of theBuchneragenome. We foundBuchnerapolymorphisms within aphid strains, suggesting the presence of genetically differentBuchnerastrains within the same clonal lineage. Genetic distance between pairs ofBuchnerasamples was positively correlated to genetic distance between their aphid hosts, indicating shared evolutionary histories. However, there was no segregation in the genetic variation of bothM. persicaeandBuchnerawith plant host (Brassicaceae and non-tobacco Solanaceae) and no associations between genetic and geographic distance at global or regional spatial scales. Abundance patterns of non-synonymous mutations were similar to synonymous mutations in theBuchneragenome, and both mutation classes had similar site frequency spectra. We hypothesize that a predominance of neutral processes results in theBuchneraofM. persicaeto simply ‘drift’ with the evolutionary trajectory of their aphid hosts. Our study presents a unique microevolutionary characterisation ofBuchnera–aphid host genomic covariation across multiple aphid clones. This provides a new perspective on the eco-evolutionary processes generating and maintaining polymorphisms in a major pest aphid species and its obligate primary endosymbiont.
Targeting pediatric High-Grade Gliomas withOAcGD2-CAR Vδ2 T cells
Authors: Thomas Pauline , Veerasamy Maëva , Devinat Marine , Guiet Elodie , Ollier Jocelyn , Paris Pierre , Entz-Werlé Natacha , Gratas Catherine , Clémenceau Béatrice , Birklé Stéphane , Paris François , Pecqueur Claire , Fougeray Sophie 
Published: November 2023

AbstractPurposePediatric high-grade gliomas (pHGG) belong to a family of rare children’s cancers which are treated with radiotherapy, based on adult high-grade glioma standard of care. However, new treatments are definitively required since actual ones are unable to extend survival by more than a few months in most patients. In this study, we investigate a Chimeric Antigen Receptor (CAR)-T cell immunotherapy targeting theOAcGD2 ganglioside, using either conventional αβ or Vδ2 T cells as effectors.Materials and methodsUsing relevant human primary models of pHGG, we first characterized the innate Vδ2 T cell immunoreactivity. Then, following the validation ofOAcGD2 expression in these tumor cells, we evaluated both αβ and Vδ2OAcGD2-CAR-T cell immunoreactivity using various methods including videomicroscopy, FACS and cytotoxicity assays.ResultsWe showed that pHGG primary cells are not spontaneously recognized and killed by Vδ2 T cells but significantly expressed theOAcGD2 ganglioside. Accordingly, both αβ and Vδ2 T cells engineered to express a CAR against theOAcGD2 efficiently killed pHGG cells in 2D and 3D models. Importantly, only Vδ2 T cells transduced with the completeOAcGD2-CAR eliminated pHGG cells, in contrast to conventional αβ CAR-T cells that killed tumor cells even in the absence of CAR expression, highlighting the allogeneic potential of Vδ2 CAR-T cells.ConclusionOur study demonstrates the preclinical relevance of targetingOAcGD2 in pHGG using CAR-T cells. Furthermore, we also clearly demonstrate the clinical benefits of using Vδ2 T cells as CAR effectors in allogeneic settings allowing an off-the-shelf immunotherapy.