bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two‐dimensional separation of peptides using RP‐RP‐HPLC system with different pH in first and second separation dimensions
Authors: Gilar Martin , Olivova Petra , Daly Amy E. , Gebler John C. 
Published: August 2005

AbstractTwo‐dimensional high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than single‐dimensional LC. The most popular 2D‐HPLC approach used today for proteomic research combines strong cation exchange and reversed‐phase HPLC. We have evaluated an alternative mode for 2D‐HPLC of peptides, employing reversed‐phase columns in both separation dimensions. The orthogonality of 2D separation was investigated for selected types of RP stationary phases, ion‐pairing agents and mobile phase pH. The pH appears to have the most significant impact on the RP‐LC separation selectivity; the greatest orthogonality was achieved for the system with C18 columns using pH 10 in the first and pH 2.6 in the second LC dimension. Separation was performed in off‐line mode with partial fraction evaporation. The achievable peak capacity in RP‐RP‐HPLC and overall performance compares favorably to SCX‐RP‐HPLC and holds promise for proteomic analysis.
Brisk deep‐tendon reflexes as a distinctive phenotype in an argentinean spinocerebellar ataxia type 2 pedigree
Authors: Rosa Alberto L. , Molina Irma , Kowaljow Valeria , Conde Cecilia B. 
Published: August 2005

AbstractSlow saccades, postural/intention tremor, peripheral neuropathy, and decreased deep‐tendon reflexes are valuable neurological signs for clinical suspicion of spinocerebellar ataxia type 2 (SCA2). We report the presence of abnormally brisk deep‐tendon reflexes in nonsymptomatic carriers and mildly and severely affected subjects of a large Argentinean SCA2 pedigree. The identification of this distinctive SCA2 phenotype in an entire pedigree reinforces the current concept that clinical algorithms are of limited value as indicators for genetic testing in SCA. Combined with published pedigrees of SCA2 manifesting as levodopa‐responsive parkinsonism, this finding suggests that modifier genes could influence the clinical phenotype of SCA2. © 2005 Movement Disorder Society
Osteoporosis in Parkinson's disease
Authors: Wood Brian , Walker Richard 
Published: August 2005

AbstractThere are few studies of osteoporosis in Parkinson's disease (PD). We assessed the prevalence of osteoporosis in a PD clinic cohort. All subjects with a confirmed diagnosis of PD attending a clinic were invited to participate. All consenting subjects had bone density measured by dual energy X‐ray absorptiometry scanning. Further data, including demography, disease duration, and disease severity, were collected. One hundred five subjects participated; median age was 75 (54–92) years. Fifty‐one (49%) patients were men. Of the men: median T score, −1.3 (range, −4.7 to 3.8); median Z score, 0.0 (−3.2 to 4.7); diagnostic categories: osteoporosis, 20%; osteopenia, 41%; normal, 39%. Of the women: median T score −2.7 (−4.7 to 1.4); median Z score, −0.25 (−2.6 to 4.2); diagnostic categories: osteoporosis, 63%; osteopenia, 28%; and normal, 9%. Whole sample: osteoporosis, 42%; osteopenia, 34%; and normal, 24%. There were associations between age, depression, disease duration, and osteoporosis but not with disease severity. Female gender was an independent predictor of osteoporosis. The prevalence of osteoporosis/osteopenia is considerable in PD patients but does not exceed that of other people of similar age. Osteoporosis/osteopenia was present in almost all women of this age group with PD. © 2005 Movement Disorder Society
Myoclonus of the scapula after acute long thoracic nerve lesion: A case report
Authors: Camerota Filippo , Celletti Claudia , Paoloni Marco , Serrao Mariano , Inghilleri Maurizio , Pierelli Francesco , Santilli Valter 
Published: August 2005

AbstractWe describe a patient who presented myoclonus in the left scapula 3 months after a traumatic lesion of the left long thoracic nerve. Myoclonic activity was recorded as pseudorhythmic electromyographic bursts repeated at a frequency of 2 to 4 Hz, each lasting between 100 and 200 msec, in the left serratus–dorsalis muscle region, trapezius, and deltoid muscles. A combination of peripheral and central mechanisms may have induced the myoclonus in this case. © 2005 Movement Disorder Society
Dystonia gravidarum: A new entity?
Authors: Lim Erle Chuen‐Hian , Seet Raymond Chee‐Seong , Wilder‐Smith Einar P.V. , Ong Benjamin Kian‐Chung 
Published: August 2005

AbstractWe describe cervical dystonia occurring in a 31‐year‐old, previously well, primigravid, primiparous Chinese woman at 4 weeks' amenorrhea, which was ameliorated with low‐dose clonazepam and disappeared completely by the end of the second trimester without recurring despite her being tapered off benzodiazepine therapy. Investigations were unremarkable for structural and biochemical causes of dystonia. Chorea, paraballismus, and restless legs syndrome are known to occur during pregnancy, attributable to high estrogen levels. Dystonia, on the other hand, has not been described to occur de novo in pregnancy. This association should be considered in women of reproductive age who present with cervical dystonia. © 2005 Movement Disorder Society
Rating scale for psychogenic movement disorders: Scale development and clinimetric testing
Authors: Hinson Vanessa K. , Cubo Esther , Comella Cynthia L. , Goetz Christopher G. , Leurgans Sue 
Published: August 2005

AbstractWe developed and tested the clinimetric properties of a scale for psychogenic movement disorders (PMDs). PMDs are disabling but lack any generally accepted treatment strategies. To develop treatments, means of assessing disease severity must be provided. No scale to assess PMDs existed. The PMD scale developed here rates 10 phenomena (rest tremor, action tremor, dystonia, chorea, bradykinesia, myoclonus, tics, athetosis, ballism, cerebellar incoordination), 2 functions (gait, speech), and 14 body regions. To study interrater agreement, three movement disorder neurologists independently rated 88 videotapes of PMD patients. Data analysis was performed using a kappa coefficient of agreement, Kendall's coefficient of concordance, Spearman correlations, and intraclass correlation coefficients. Validity and scale responsiveness were tested as well. All phenomena and speech and gait dysfunction occurred in the patient sample. A wide range of affected body regions, severity, and incapacitation was captured. Ratings showed excellent interrater reliability for presence or absence of each phenomenon (κ range, 0.63 to 0.86). Kendall's concordance coefficients for phenomenology, function, and total PMD scores were 0.92, 0.93, and 0.91. Spearman correlations between raters ranged from 0.86 to 0.90. The scale was responsive to changes that occurred as a result of a neuropsychiatric intervention. The PMD scale adequately captures the complex movements of PMDs and can be used to assess PMDs and test the efficacy of intervention strategies. © 2005 Movement Disorder Society
Alteration of central motor excitability in a patient with hemimasticatory spasm after treatment with botulinum toxin injections
Authors: Mir Pablo , Gilio Francesca , Edwards Mark , Inghilleri Maurizio , Bhatia Kailash P. , Rothwell John C. , Quinn Niall 
Published: August 2005

AbstractHemimasticatory spasm (HMS) is a condition characterized by paroxysmal involuntary contraction of masticatory muscles. We performed an electrophysiological investigation of a single patient with HMS to identify any pathophysiological changes associated with the condition. We identified a delayed M wave and jaw jerk on the affected side and an absent masseteric silent period during spasm. Botulinum toxin injections successfully treated the clinical symptoms and resulted in a significant reduction in the excitability of the blink reflex recovery cycle. These data suggest that HMS may be due to ectopic activity in the motor portion of the trigeminal nerve that is capable of inducing changes in the excitability of central reflex pathways. These changes can be altered by successful treatment with botulinum toxin. © 2005 Movement Disorder Society
Staged bilateral stereotactic pallidothalamotomy for life‐threatening dystonia in a child with Hallervorden–Spatz disease
Authors: Balas Istvan , Kovacs Norbert , Hollody Katalin 
Published: August 2005

AbstractHallervorden–Spatz disease (HSD) is a rare disorder characterized by progressive motor dysfunction and dementia. Dystonia is the most prominent and disabling symptom, responding only to a modest extent to pharmacological therapy. At the moment, only a few cases have been reported to improve dystonia and even fewer to resolve status dystonicus for a longer period in children. The authors present the case of a 10‐year‐old boy who had progressive generalized dystonia, resulting in spontaneous femur fracture and life‐threatening swallowing and respiratory disability. As a rescue solution, staged bilateral pallidothalamotomy was performed. Postoperatively, Burke–Fahn–Marsden Dystonia Rating Scale and Dystonia Disability Rating Scale improved (from 116 and 30 points to 41 and 18 points, respectively) and painful dystonia was resolved, which was still continuous 4 years later (47 and 20 points). Stereotactic staged bilateral pallidothalamotomy should be considered as a potential treatment in the management of life‐threatening generalized dystonia related to HSD. © 2005 Movement Disorder Society
Use of complementary therapies in patients with Parkinson's disease in Singapore
Authors: Tan Louis C.S. , Lau Puay‐Ngoh , Jamora Roland Dominic G. , Chan Edwin S.Y. 
Published: August 2005

AbstractTo determine the frequency and spectrum of complementary therapy (CT) use and its association with sociodemographic or disease‐specific characteristics among Asian patients with Parkinson's disease (PD) in Singapore, we interviewed 159 patients using a structured questionnaire. Sixty‐one percent (95% CI = 55–67) of participants used at least one type of CT for PD, of which the most common were traditional medicine, acupuncture, and vitamins/health supplements. Among CT users, 40% (95% CI = 32–48) subjectively reported some improvement of their symptoms. However, only 16% informed their physicians of their use of CT. Due to unequal follow‐up times, a survival analysis approach was adopted for statistical analysis. The rate of starting CT use was 1.2/100 person months. At 3 years after PD diagnosis, 48% had started using CT. Among a subgroup of participants, those with a baseline Unified Parkinson's Disease Rating Scale motor score of more than 16 were 2.5 times more likely to use CT compared to those with a baseline score of 16 or less (P = 0.031; 95% CI = 1.1–5.8). CT use was not associated with age of onset of PD or other sociodemographic factors. The use of CT is high among Asian PD patients. Patients with more severe motor dysfunction at onset are more likely to use CT. © 2005 Movement Disorder Society
Deep brain stimulation to treat hyperkinetic symptoms of cockayne syndrome
Authors: Hebb Matthew O. , Gaudet Paula , Mendez Ivar 
Published: August 2005

AbstractCockayne syndrome manifests a spectrum of neurological dysfunction that includes medically intractable movement disorders. Deep brain stimulation has not been well studied in such rare neurodegenerative conditions. In this case, stimulation of the ventral intermediate nucleus of the thalamus was used to manage severe motor symptoms in a young man with Cockayne syndrome. There was a marked and progressive response to thalamic stimulation within weeks of surgery. These results suggest that patients with Cockayne syndrome should be considered for deep brain stimulation to treat refractory movement disorders. © 2005 Movement Disorder Society
Homocysteine and serum markers of immune activation in primary dystonia
Authors: Muller Ulf J. , Frick Barbara , Winkler Christiana , Fuchs Dietmar , Wenning Gregor K. , Poewe Werner , Mueller Joerg 
Published: August 2005

AbstractThe cause of primary dystonia remains unknown. Several reports point to immune system disturbances in primary dystonia and a recent study demonstrated hyperhomocysteinemia in cervical dystonia. Homocysteine (HCY) is an amino acid and elevated HCY concentrations were shown to be associated with immune system activation and increased neopterin serum concentrations. We examined HCY serum concentrations together with serum markers of immune activation in patients with different types of primary dystonia. Eighty‐three patients with different types of primary dystonia were included and investigated at least 3 months following botulinum toxin treatment. Thirty‐six healthy volunteers with similar age and sex distribution served as controls. Total serum HCY, kynurenine, and tryptophan concentrations were determined by high‐performance liquid chromatography; neopterin, folate, and vitamin B12 concentrations were measured by immunoassays. Routine blood analysis, including C‐reactive protein (CRP), erythrocyte sedimentation rate (ESR), and white blood count (WBC), was performed. Patients with primary dystonia had significantly higher HCY concentrations compared to controls. Among the dystonia subtypes, no significant difference of HCY serum concentrations was observed. CRP and ESR were within the normal range in >90% of the patients and all had normal WBC. Neopterin, kynurenine, and tryptophan serum concentrations were similar in patients and controls and not correlated with HCY serum concentrations. The results provide evidence against enhanced cellular immune activation in patients with primary dystonia. However, hyperhomocysteinemia was present in all dystonia subtypes and unrelated to immune activation in this study. HCY is a neuronal excitotoxic amino acid and hyperhomocysteinemia is considered an independent vascular risk factor. Further studies are required to define the background of hyperhomocysteinemia in primary dystonia. © 2005 Movement Disorder Society
In vivo imaging of microglial activation with [11C](R)‐PK11195 PET in progressive supranuclear palsy
Authors: Gerhard Alexander , Trender‐Gerhard Iris , Turkheimer Federico , Quinn Niall P. , Bhatia Kailash P. , Brooks David J. 
Published: August 2005

AbstractProgressive supranuclear palsy (PSP) is a neurodegenerative disease presenting with voluntary gaze difficulties, early falls, and Parkinsonism. Neuronal loss, associated with intracellular neurofibrillary tangles and activated microglia, is found targeting the basal ganglia, brainstem nuclei, and frontal cortex. [11C](R)‐PK11195 PET is a marker of peripheral benzodiazepine binding sites (PBBS) expressed by activated microglia. We have used [11C](R)‐PK11195 PET to demonstrate in vivo the degree and distribution of the glial response to the degenerative process in four patients with PSP. Compared to normal age‐matched controls, the PSP patient group showed significantly increased mean [11C](R)‐PK11195 binding in the basal ganglia, midbrain, the frontal lobe, and the cerebellum. Two of the patients were rescanned after 6 to 10 months and during that time the level of microglial activation remained stable. [11C](R)‐PK11195 PET reveals a pattern of increased microglial activation in PSP patients involving cortical and subcortical regions that corresponds well with the known distribution of neuropathological changes. [11C](R)‐PK11195 PET, therefore, may help in characterizing in vivo the underlying disease activity in PSP. © 2005 Movement Disorder Society
Capacity allocation with machine duplication in semiconductor manufacturing
Authors: Çatay Bülent , Erengüç Ş. Selçuk , Vakharia Asoo J. 
Published: August 2005

AbstractThe manufacturing process for a computer chip is complex in that it involves a large number of distinct operations requiring a substantial lead‐time for completion. Our observations of such a manufacturing process at a large plant in the United States led us to identify several tactical and operational problems that were being addressed by the production planners on a recurring basis. This paper focuses on one such problem. At a tactical level, given a demand forecast of wafers to be manufactured, one specific problem deals with specifying which machine or machine groups will process different batches of wafers. We address this problem by recognizing the capacity limitations of the individual machines as well as the requirement for reducing operating and investment costs related to the machines. A mathematical model, which is a variation of the well‐known capacitated facility location problem, is proposed to solve this problem. Given the intractability of the model, we first develop problem specific lower bounding procedures based on Lagrangean relaxation. We also propose a heuristic method to obtain “good” solutions with reasonable computational effort. Computational tests, using hypothetical and industry‐based data, indicate that our heuristic approach provides optimal/near optimal solutions fairly quickly. © 2005 Wiley Periodicals, Inc. Naval Research Logistics, 2005
How do patients who participate in cancer support groups differ from those who do not?
Authors: Grande G.E. , Myers L.B. , Sutton S.R. 
Published: August 2005

AbstractResearch has shown that cancer patients in general benefit from support group participation. However, few patients attend such groups. This study investigated differences between participants of a community cancer support group and a random selection of non‐participants from the Cancer Registry. Data were collected through mail survey, and included variables identified through past research and variables derived from Leventhal's self‐regulatory model of illness representations and the theory of planned behaviour. Sixty‐three support group participants and 44 comparison sample respondents were recruited. Support group participants were more likely to be female, without a partner, younger, and to have more education and formal support than non‐participants. They held more favourable views of support groups, believed that significant others were favourable towards participation, and perceived less difficulty in joining a group. They furthermore used more active, adaptive coping strategies and felt more control over their cancer, but were more distressed and anxious. Non‐participants reported more support from a special person. A multivariate logistic regression analysis showed only psychosocial variables to be independent predictors of participation. As psychological variables are amenable to change, increase of appropriate support group participation should be possible, for instance by addressing patients' beliefs about support groups. Copyright © 2005 John Wiley & Sons, Ltd.
Support‐seeking, perceived support, and anxiety in mothers and fathers after children's cancer treatment
Authors: Norberg Annika Lindahl , Lindblad Frank , Boman Krister K. 
Published: August 2005

AbstractThe objective was to examine the relationships between anxiety, the seeking of social support as a coping strategy, and perceived social support among mothers (n=103) and fathers (n=81) of children with successfully completed treatment for cancer. Assessments were done using self‐report instruments. The mediating effect of perceived support on the relationship between social support‐seeking and anxiety was evaluated through path analysis, and comparisons were done in order to evaluate effects of gender.For mothers and fathers alike, a positive relationship of moderate strength between support‐seeking and perceived support was found. Anxiety was negatively related to support‐seeking (mothers r=−0.22, p=0.025; fathers r=−0.21, p=0.063) as well as perceived support (mothers r=−0.55, p<0.001; fathers r=−0.41, p<0.001), although the relationship for support‐seeking was weak. The path analysis showed that perceived support only to a minor extent could strengthen this association. The significance of support‐seeking and perceived support was stronger for mothers than for fathers, as regards their association with anxiety. However, the patterns of interrelations were similar for mothers and fathers. In conclusion, parents' subjectively perceived support appears to be more important for anxiety regulation than their support‐seeking coping. In clinical practice, individual variation should be acknowledged, and presumptions of general gender differences avoided. Copyright © 2005 John Wiley & Sons, Ltd.
System behaviour of wood truss assemblies
Authors: Gupta Rakesh 
Published: August 2005

AbstractWood truss assemblies are widely used in light frame construction all over the world. Although the volume of literature on single trusses and joints is relatively huge, the system behaviour of truss assemblies has received only limited attention. The main approach to take into account the system behaviour of an assembly is to use a system factor in the design of single trusses. However, technical advances now make it possible to analyse and design these complex assemblies as systems using three‐dimensional structural analysis programs, thereby including the system effect directly. This paper presents an overview of previous research on system behaviour of truss assemblies and recommends a system design approach to designing truss assemblies.
Binding energy of impurity states in a parabolic quantum dot in a strong magnetic field
Authors: Peter A. John , Gnanasekar K. , Navaneethakrishnan K. 
Published: August 2005

AbstractThe binding energies of shallow hydrogenic impurities in parabolic quantum dots are calculated using a variational approach within the effective mass approximation. Results are obtained for CdMnTe/CdMnTe structures as a function of the dot radius in an external magnetic field. The results show that the impurity binding energy (i) increases with the decrease in dot radius, (ii) decreases when the magnetic field is increased for a given dot and (iii) increases to a maximum value at 90 Å and then decreases as the size of the dot increases beyond 90 Å in the finite barrier case. This feature is different from non magnetic quantum well structures. Spin polaronic shifts are estimated using a mean field theory. The results show that the shifts are ≤1 meV in the zero‐field case and still smaller in a magnetic field. This shift not only decreases in a magnetic field but also decreases when the dot radius increases. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Kinetic Ising model in two and three dimensions with constraints
Authors: Kuhlmann Claudia , Trimper Steffen , Schulz Michael 
Published: August 2005

AbstractWe present the dynamics of the kinetically constrained Ising model, comprised of a system of spins coupled with the strength J and situated in a field which plays the role of activation energy. Due to kinetic constraints, glassy effects arise at low temperatures leading to a non‐Arrhenius α‐relaxation time. The results of Monte Carlo simulations are presented for the 2D and 3D facilitated kinetic Ising model with nonzero coupling J. The spin autocorrelation function exhibits, in a broad intermediate time regime, a pronounced stretched exponential decay characterized by an exponent γ. Whereas the relaxation time depends strongly on the activation energy and the interaction strength J, we find a non‐universal stretching exponent γ depending on the temperature and on J. In 3D the autocorrelation function shows a crossover into a pure exponential decay consistent with theoretical predictions. The magnetization is also analyzed and identified as a control parameter of the system. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)