bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing Multi-epitope Antigen Construct from Immunodominant
Proteins for Serological Diagnosis of Chlamydia trachomatis: An In Silico
Approach
Authors: Ghalamfarsa Farideh , Savardashtaki Amir , Irajie Cambyz , Emami Amir , Nezafat Navid , Ghasemi Younes 
Published: November 2023

Background:
Chlamydiasis is a widespread bacterial infection in the world. Serological
tests are expensive, and in addition, intrinsic antigens can cause cross-reactions and make the diagnosis
process difficult. Multi-epitope protein antigens are novel and potential diagnostic markers
that have the capability of more accurate and cheaper diagnosis. Therefore, in this study, the main
goal is to design a new protein vaccine, including multiple epitopes of B cells with dominant immunity
from three proteins named MOMP, ompA and Pgp3D from C. trachomatis

Methods:
The amino acid sequences were obtained from the UniProt database. The areas with the
highest antigenicity were identified using the EMBOSS server. Linear B cell epitopes were determined
using BCPRED, ABCpred, and Bepipred servers. Epitopes with the highest antigenicity were
connected using the EAAAK linker.

Results:
Two epitopes from MOMP, two from ompA, and one from Pgp3D were selected. These
epitopes were connected to each other with the EAAAK linker. Three residues (0.592), 16 residues
(0.76), 36 residues (0.578), and 37 residues (0.734) were obtained from the prediction of the spatial
structure of the B cell multiple epitopes designed with ElliPro. Model 1 of RaptorX was selected as the
best structure. In this model, the ERRAT quality, ProSA-web z-score, and Verify3D were 83.1169, -
5.17 and 84.62% with PASS score, respectively. Moreover, the Ramachandran plot showed that
86.093% of the amino acid residues were located in the favored region. To achieve the highest level of
protein expression, the designed multi-epitope reverse-translated with the Genscript server and was
cloned in E. coli. The highest level of expression was achieved, and a CAI score of 0.91 was reported.
The gene GC content was 51.98%, and the contribution of low-frequency codons was 0%.

Conclusion:
The results confirmed that the designed construct could identify C. trachomatis with
high sensitivity and specificity in serum samples of patients with chlamydiasis. However, further
experimental studies are needed for final confirmation.
End result of physical exercise with pathological increase in Lactic Acid
Authors: Dharam Chand  , Avadhesh Kumar  , Deena Nath Singh  , Shweta Mishra  
Published: October 2023

Ayurveda advises adhering to the practices described by our Acharyas for well being. Ritucharya offers guidance on food plans that are suitable for overcoming the effects of seasonal fluctuations. We must adhere to the Ayurvedic prescriptions in order to maintain our mental, physical, and nutritional wellness. Between around mid-May and mid-July is the Grishma (summer) season. It is common for the ecosystem to experience intense heat and dangerous wind, and then comes the rain with heat and humidity thus the strength of individual gets decreased. According to Acharya Charak, the Vayayam is stated in Sutra Shana 7/31, which mentions that keeping the body in accordance with will power through engaging in Yogasana. For the appropriate maintenance of physical health many Acharya have explained that in Grishma Ritu exercise should be performed at half the level of actual strength and up to when sweet appear on forehead. The body's lactic acid levels rise during activity, which causes an oxygen level reduction. Exercise-induced lactic acidosis is often a temporary condition. The body's pH level drops to 7.4 to 6.6. Lactic acid levels are decreased by vitamin C, magnesium, and drinking water. Exercise also causes lactic acid levels to drop after an hour. There are many ill effect of excess lactic acid on different organs as liver, kidney, lungs, heart, muscle etc. Any source of sea food, as well as beans, leafy greens, nuts and seeds, salmon, fish and mackerel, keeps the amount of lactic acid stable.
Comparación de indicadores microbiológicos en sistemas de cultivo de tomate convencional o hidropónico
Authors: Wittmann Vega Viviana , Davidovich-Young Gabriela , Wong-González Eric , Montero Barrantes Manuel 
Published: October 2023

Introducción: El consumo de tomate fresco ha ido en aumento a través de los años y los sistemas de cultivo (convencional o hidropónico), las prácticas de cosecha y poscosecha, el agua de riego y los contenedores pueden incidir sobre su calidad microbiológica. Objetivo: Comparar la calidad microbiológica de la fruta cultivada o cosechada, del agua de riego y de los contenedores de cosecha en dos fincas de producción de tomate (convencional e hidropónica). Métodos: Realizamos tres muestreos a cada finca (repeticiones), tomando, en cada repetición, 3 tomates de cada tipo que conformaron una muestra compuesta, 50 ml de agua de riego e hisopando 50cm2 de la superficie de tres contenedores de cosecha (muestra compuesta). Determinamos los indicadores microbiológicos de recuento total aerobio mesófilo, recuento de hongos filamentosos y levaduras, coliformes totales, coliformes termotolerantes y E. coli. Además, analizamos la presencia de Listeria monocytogenes en los frutos. Aplicamos pruebas de t-student a un nivel de significancia del 5%. Resultados: Los recuentos total aerobio mesófilo y hongos filamentosos y levaduras del tomate hidropónico fueron significativamente menores que en el tomate convencional, mientras que no hubo diferencia en los recuentos de coliformes totales y E. coli, además L. monocytogenes estuvo ausente en todas las muestras. El recuento total aerobio mesófilo fue mayor en los contenedores de cosecha de la finca convencional. En ambas fincas el agua de riego no se consideró fuente de contaminación pues tuvo niveles muy bajos de coliformes totales, coliformes termotolerantes y E. coli. Finalmente, se presume un control adecuado en las fincas durante la cosecha, al no haber encontrado diferencia en los indicadores microbiológicos entre el tomate cultivado y el cosechado. Conclusión: Los tomates hidropónicos presentan mayor probabilidad de tener un mejor perfil microbiológico y mayor vida útil en comparación con los tomates cultivados de forma convencional, evidenciando la importancia del control ambiental y la limpieza y desinfección de todos los insumos utilizados en los procesos de cultivo, cosecha y poscosecha.
Eye in the Sky: Hyperspectral Imaging for Sustainable Nitrogen Management in Vegetables
Authors: Gill Taqdeer , Townsend Philip A. , Wang Yi 
Published: October 2023

Snap beans and kidney beans are poor nitrogen fixers and need nitrogen
(N) fertilizer. However, excessive application of N leads to groundwater
contamination. The traditional way of measuring crop nitrogen status is
destructive and time-consuming. The objective of this study was to
develop a tool that accurately predict the real-time crop nitrogen
status and the end-of season yield for optimizing fertilizer management.
The field trial was conducted in 2022 and 2023. Eight nitrogen
treatments were applied at 22 kg ha, 56 kg
ha, 84 kg ha, 112 kg
ha, 140 kg ha, 168 kg ha-1, 196
kg ha, 224 kg ha to three kidney
beans cultivars. Six nitrogen treatments were applied at 22 kg
ha, 56 kg ha, 84 kg
ha, 112 kg ha, 140 kg
ha, 168 kg ha to two snap beans
cultivars. Hyperspectral images (400 nm to 2500 nm) were collected on a
weekly basis. Top twenty bands along with genotype (cultivars),
environmental factors (temperature, precipitation, growing Degree Days),
management factors (nitrogen rate, days after planting, irrigation) were
used to train different machine learning algorithms including linear
regression, random forest, XG Boost, support vector machine and
k-nearest neighbors for predicting the nitrogen status and the final
yield.
Our results indicated that top twenty bands along with GEM performed the
best for predicting final yield (R as high as 0.82
and RMSE as low as 1.6). Our study demonstrated the potential capacity
of hyperspectral imaging and machine learning models to estimate crop
yield and nitrogen status.
Modifikasi Alat Perajang Batang Singkong (Rabakong Tipe Tep 5) untuk Meningkatkan Kapasitas Kerja
Authors: Asmara Sandi , Kuncoro Sapto , Suharyatun Siti , Setiawan Firnando Anggi 
Published: October 2023

Lampung Province is the largest producer of cassava in Indonesia. However, cassava production in Lampung has been declining over the past five years due to many farmers switching to other commodities due to the long harvesting period and unstable prices. This has resulted in a buildup of cassava stem waste, requiring innovation to manage this waste. Hence, the cassava stem chopper (Rabakong) was introduced. Rabakong has been developed by Agricultural Engineering (TEP) students from the University of Lampung, starting from TEP 1, TEP 2, TEP 3, TEP 4, and TEP 5. This research method involves modifying the frame of the previous version, redesigning the components to be modified using AutoCAD software, and then fabricating and collecting data after modification. The latest modified Rabakong machine underwent significant changes in the number of blades, which are fewer but arranged spirally for efficiency in chopping and space-saving. The working capacity has increased to 207 kg/hour at 1400 rpm. Waste material has decreased to 11.39%. Fuel consumption is 2.580 liters/hour. The percentage of finely chopped materials (<0.2 mm) is only 17.19%, while coarsely chopped materials (>0.5 cm) reach 51.41%, indicating that the latest version is more suitable for coarse results.. Keywords: Cassava Stems, Design, Efficiency, Modification.
Pengaruh Variasi Bahan Peredam Kemasan dan Tingkat Kematangan Buah Alpukat terhadap Tingkat Kerusakan Mekanis pada Simulasi Getaran Selama Transportasi
Authors: Rahmah Fadhlilla Monica , Tamrin Tamrin , Rahmawati Winda , Kuncoro Sapto 
Published: October 2023

Cause of damage is improper or inappropriate packaging. Therefore this study aims to determine the optimal type of packaging damper in minimizing mechanical damage to the fruit. This research was conducted in February - April 2020 at the Laboratory of Bioprocess Engineering and Postharvest Handling, University of Lampung. This research was conducted by looking at the level of damage to the avocado when dropped manually and when vibrated mechanically with a vibrating table. In the manual experiment, one avocado is divided into four parts, each part will be treated with different absorbent materials in the form of straw, fibers, dried banana leaves and control with a thickness of 2cm damper which is then dropped from a height of 5 cm, 10 cm, and 15 cm. In mechanical testing using a packaging box measuring 29 cm long, 14 cm wide and 14 cm high. Each box contains 12 avocados arranged in 2 layers and then added with a damper with a thickness of 2 cm, each package is ventilated with a circle type with a diameter of 3 cm, 4 pieces on each right and left side of the package. The observed parameters were mechanical damage in the form of bruises, weight loss, and level of hardness test. The observed data obtained were processed using simple statistics with each data averaged and compared to the damage between the fruit that was dropped manually and vibrated with a vibrating table. The results showed that the fiber-absorbing agent treatment was more optimal in minimizing mechanical damage to avocado because the fibers had a lignin content of around 29.4%. Keywords: Avocado, Mechanical Damage, Silencer, Transportation Simulation.
The Cognitive, Affective, and Psychomotor Domain on English Lesson Plan in School Based Curriculum
Authors: Handayani Irma , Mukhaiyar Mukhaiyar , Syarif Hermawati 
Published: November 2023

This research is focus to analyze teachers in implementing three domains that is cognitive, affective, and psychomotor that suggested by Bloom. Then problems that feced by teachers in implementing these three domian in teaching and learning process. this is qualitative research in case study approach. Data of this research were get from observation and interview three teachers whose teach English subject in Senior High School 5 Padang West Sumatra Indonesia. Base on data that researcher have got, researcher found that: first teachers are not well prepare lesson plan. This is seen in cognitive domain (36.6%) then affective (27.6%) and psychomotor (35.7%). In general from percentage above, result of education still not maximal. Second, there are three problems that faced by teachers in teaching and learning proces, they are: (i) limit of time implementing three domain, (ii) ability of each students in english subject and (iii) limitation of teachers in understanding in implementing this currriculum because of lack training.
Akta Partij Berdasarkan Undang- Undang Nomor 2 Tahun 2014 Tentang Jabatan Notaris Dalam Rangka Perwujudan Kepastian Hukum
Authors: Yonnawati Yonnawati , Sukardi Dina Haryati , Jehaniza Ulan 
Published: October 2023

Penelitian ini di dalamnya mengkaji serta menjawab permasalahan tentang akta partij di hadapan notaris dan kepastian dari hukum partij akta yang dilaksanakan ataupun dinyatakan di depan notaris. Penelitian juga masuk pada kelompok penelitian yuridis empiris yang bersifat normatif. Bahan yang telah diperoleh melalui penelitian pada kantor Notaris Cindy Larasaty, S.H., M.Kn. dan pembahasan yang sudah dilakukan adalah partij akta menurut Undang-Undang No 2 Tahun 2014 merupakan akta pihak-pihak yang dilaksanakan di depan notaris untuk kemudia di autentikan dengan tandatangan notaris. Perbedaan partij akta dan akta yang tidak resmi merupakan proses pembuatannya, partij akta di nyatakan serta dilaksanakan di depan notaris kemudian diparaf dengan notaris sedangkan akta di bawah tangan cuma atas kedua pihak, sifat-sifat yang memenuhi unsur akta partij merupakan : akta musti dilaksanakan dari pihak-pihak langsung di depan notaris, akta musti dilaksanakan bertara kepada bentuk yang dimuat oleh Undang-undang, akta yang dilaksanakan terhadap ataupun didepan pejabat publik yang berkompeten dalam kausa itu serta tempat akta tersebut dilaksanakan. memenuhi bagian-bagian pada Pasal 1320 KUHPerdata. Notaris pun harus memasukan akta yang dibuat ke dalam buku besar notaris kemudian nantinya akan dievaluasi setiap tahunnya. Untuk dipastikan kebenarannya, notaris harus menjaga kerahasiaan dari pihak-pihak yang menghadap. Hal yang sering dilanggar baik oleh para pihak maupun notaris seperti notaris yang kurang teliti, notaris mendokumentasikan akta kemudian disebar dimedia sosial, kurangnya pemahaman bahasa asing. Sedangkan dari para pihak klien pihak- pihaknya tidak datang bersamaan, para pihak tidak memahami dampak dari kesepakatan. Solusi yang dapat diberikan kepada notaris yaitu dengan cara lebih memfokuskan pada syarat pembuktian formil akta.
Analisis Yuridis Pembinaan Residivis Anak Pada Lembaga Pemasyarakatan Khusus Anak
Authors: Aryani Gustina 
Published: October 2023

The development of prisoners in Indonesia is implemented in the correctional system. Various forms of guidance for correctional students at Special Child Development Institutions cannot be separated from a change in the correctional system, which aims to provide provisions for correctional students to face life after completing their sentence. The problems that will be examined in this article include how to foster child recidivism in Special Child Development Institutions and what are the inhibiting factors in the implementation of child recidivism guidance in Special Child Development Institutions.
The method used in this research is Normative Juridical and Empirical Juridical Research Methods, using primary data and secondary data.
Research Results: The Bandar Lampung Special Children's Development Institute has generally attempted to provide rights to children by implementing and providing guidance in accordance with what is mandated by law. The implementation of coaching for correctional students who are recidivists has so far been the same as other correctional students who are not recidivists, this is because the program and coaching patterns that have existed so far have regulated this. In its implementation, Special Child Development Institutions find it quite difficult to run a coaching program, several inhibiting factors in the implementation of recidivist coaching for correctional students include: low level of education, children's skill abilities, lack of community participation and children's changing thinking patterns. Suggestions that can be given by the author regarding the problems that arise include: for Special Child Development Institutions, it is necessary to differentiate between the training of ordinary correctional students and recidivist correctional students which can have a deterrent effect on children so that they do not commit crimes again, for example by developing their mental health. children and put more emphasis on spiritual development and for officers at Special Education Institutions for Children to be able to encourage correctional students not to commit criminal acts again.
PENGARUH STRATEGI MARKETING PADA APLIKASI SHOPEE TERHADAP MINAT BELI KONSUMEN
Authors: SAVIRA NADILA 
Published: October 2023

Abstrak.Penelitian ini bertujuan untuk menganalisis pengaruh strategi marketing pada aplikasi shopee terhadap minat beli konsumennya. Penelitian ini menggunakan pendekatan kuantitatif dengan pengumpulan data melalui kuesioner. Responden berjumlah 37 orang yang terdiri dari mahasiswa. Hasil pengujian menunjukkan nilai P Values x1 sebesar 1,799 dengan nilai signifikansi 0,000 0,05 yang berarti bahwa terdapat pengaruh positif dan signifikan variabel strategi marketing terhadap minat beli konsumen . Hasil pengujian sejalan dengan hipotesis yang telah dibuat dimana pengaruh strategi marketing terhadap minat beli konsumen adalah positif dan signifikan. Nilai t-hitung sebesar 2,261 sedangkan t-tabel sebesar 0,024. Jika t-hitung t-tabel maka nilai signifikannya lebih kecil dari 5% dan hipotesis diterima. Hal ini berarti bahwa semakin baik strategi marketing yang dibuat maka akan semakin meningkatkan minat konsumen tersebut Kata kunci: shopee, strategi marketing, minat beli konsumen Abstract.This study aims to analyze the effect of the marketing strategy on the shopee application on consumer buying interest. This study used a quantitative approach by collecting data through questionnaires. Respondents amounted to 37 people consisting of students. The test results show that the P Values x1 is 1.799 with a significance value of 0.000 0.05, which means that there is a positive and significant influence of marketing strategy variables on consumer buying interest. The test results are in line with the hypothesis that has been made where the effect of marketing strategy on consumer buying interest is positive and significant. The t-count value is 2.261 while the t-table is 0.024. If t-count t-table then the significant value is less than 5% and the hypothesis is accepted. This means that the better the marketing strategy that is made, the more it will increase consumer interest
RISK FACTORS FOR THE DEVELOPMENT OF VENOUS THROMBOEMBOLISM IN PATIENTS WITH UNCOMPLICATED ACUTE CALCULOUS CHOLECYSTITIS
Authors: Kavka Mariya 
Published: October 2023

The aim is to identify and analyze risk factors for venous thromboembolism in patients diagnosed with uncomplicated acute calculous cholecystitis.
Materials and Methods. A prospective analysis of clinical data of 71 patients diagnosed with uncomplicated acute calculous cholecystitis was conducted. Two clinical groups were formed: subgroup 1A (n=6) included patients diagnosed with venous thromboembolism, and subgroup 1B (n=65) included patients without venous thromboembolism.
Results and Discussion. During the study, the coagulation potential of the patients was studied. The prothrombin index on the third day of the postoperative period and at the time of discharge was significantly higher in subgroup 1A patients (109.1% and 123.0%) compared to subgroup 1B patients (98.0% and 96.4%). The situation with total fibrinogen is similar: the mean level of this indicator during the entire observation period was significantly higher in patients of subgroup 1A (p<0.05). The mean D-dimer level at the time of hospital discharge in subgroup 1A was 3.6 mg/ml higher compared to the level of this indicator on the first day after surgery, and in subgroup 1B it was only 0.5 mg/ml higher. The risk of venous thromboembolism according to the Caprini score in subgroup 1A was 7.5 [5.5; 9.0] points, compared to 6.0 [4.0; 8.0] points in subgroup 1B. Assessment of the risk of venous thromboembolism according to the Rogers scale showed similar results: 8.0 [6.3; 9.8] points for patients of subgroup 1A and 7.0 [6.0; 8.0] points for subgroup 1B. When assessing the interleukin 1 beta (IL1b) level, its increased value was detected in subgroup 1A - 14.0 [7.0; 33.7] pg/ml, as opposed to subgroup 1B, where it was 9.0 [3.5; 26.2] pg/ml. Similar results were found in the assessment of tumor necrosis factor TNF: the mean values of this cytokine level were also higher in subgroup 1A (13.9 [7.9; 15.8] pg/ml) than in subgroup 1B (8.8 [6.0; 13.1] pg/ml).
Conclusions. According to the results of the study, the following factors for the development of venous thromboembolism in patients with uncomplicated acute calculous cholecystitis were determined: elevated levels of indicators of total fibrinogen, D-dimer, interleukin 1 beta (IL1b) and tumor necrosis factor (TNF) at the time of admission. The mean values of the total risk assessment points according to the Caprini score and Rogers scale were significantly higher (p<0.05) in the subgroup of patients with uncomplicated acute calculous cholecystitis who later developed venous thromboembolism.