bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation, Characterization, and Anticancer Activity of PEGylated Nano Liposomal Loaded with Rutin against Human Carcinoma Cells (HT‐29)
Authors: Al‐Ekaid Noorin Manhal , Al‐Samydai Ali , Al‐deeb Ibrahim , Nsairat Hamdi , Khleifat Khaled , Alshaer Walhan 
Published: October 2023

AbstractThe abstract discusses the development of rutin‐loaded nanoliposomes and their anti‐colorectal cancer activity against human carcinoma cells (HT‐29). The study characterizes the nanoliposomes using the thin‐film hydration method and analyzes their size, charge, and polydispersity index. The encapsulation efficiency and drug loading ability of rutin at different concentrations were investigated. The nanoliposomes were found to be stable for up to one month at 4 °C and showed sustained drug release for up to 24 h. The anti‐cancer activity of the rutin‐loaded nanoliposomes was found to be concentration‐dependent and significantly improved compared to free rutin. PEGylated nanoliposomes with rutin (1.8 mg/ml) showed the highest encapsulation efficiency and drug loading ability, along with improved selectivity against cancer cells. Overall, the study provides important insights into the potential use of rutin‐loaded nanoliposomes for the treatment of colorectal cancer.
Molybdenum‐based Nanocatalysts for CO Oxidation Reactions in Direct Alcohol Fuel Cells: A Critical Review
Authors: Yeetsorn Rungsima , Kumar Yogesh Gaurav , Wanchan Waritnan , Koinkar Pankaj , Yadav Kamlesh 
Published: October 2023

AbstractCarbon monoxide (CO) oxidation is crucial in fuel cell anodes. Recent research has focused on electrocatalysts that synergistically enhance CO oxidation alongside alcohol/hydrogen oxidation. High sensitivity and selectivity for CO oxidation at lower onset potentials are the key objectives. Molybdenum (Mo) has emerged as a promising non‐noble transition metal co‐catalyst for CO oxidation. Mo versatility arises from its ability to alloy with Pt and mix with other non‐noble transition metals in various forms (MoOx, MoS2, MoC). Carbon‐supported Mo nanoparticles have shown potential in reducing Pt loading and improving CO tolerance due to Mo oxyphilic properties, effectively oxidizing weakly adsorbed CO and lowering onset and peak potentials. However, challenges persist, such as a limited potential window for CO oxidation and decreased CO adsorption affinity at higher potentials. Addressing these issues requires understanding factors affecting Mo‐based electrocatalysts (Mo‐ECs) activity, including PtMo alloy composition, Mo′s chemical state, cell temperature, and the role of carbon support. This article provides a comprehensive review of Mo‐ECs role in CO electrooxidation in direct alcohol fuel cells (DAFCs) over the past two decades.
Predicting Blomia tropicalis allergens using a multiomics approach
Authors: Hubert Jan , Vrtala Susanne , Sopko Bruno , Dowd Scot E. , He Qixin , Klimov Pavel B. , Harant Karel , Talacko Pavel , Erban Tomas 
Published: October 2023

AbstractBackgroundThe domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite has not been sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis thus far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by the World Health Organization (WHO)/IUIS in domestic astigmatid mites suggest the presence of a large set of additional allergens.MethodsHere, we employ a multiomics approach to assess the allergome of B. tropicalis using genomic and transcriptomic sequence data and perform highly sensitive protein abundance quantification.FindingsAmong the 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was not found) and identified 16 potentially novel allergens based on sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among these groups, five allergen‐encoding genes are highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces).InterpretationWe report the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label‐free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that the expression levels of allergen‐encoding genes may not be strictly correlated with the actual allergenic protein abundance in mite bodies.
Altitude, latitude and climate zone as determinants of mountain hare (Lepus timidus) coat colour change
Authors: Stokes Allan W. , Hofmeester Tim R. , Thorsen Neri H. , Odden John , Linnell John D. C. , Pedersen Simen 
Published: October 2023

AbstractLocal adaptation to annually changing environments has evolved in numerous species. Seasonal coat colour change is an adaptation that has evolved in multiple mammal and bird species occupying areas that experience seasonal snow cover. It has a critical impact on fitness as predation risk may increase when an individual is mismatched against its habitat's background colour. In this paper, we investigate the correlation between landscape covariates and moult timing in a native winter‐adapted herbivore, the mountain hare (Lepus timidus), throughout Norway. Data was collected between 2011 and 2019 at 678 camera trap locations deployed across an environmental gradient. Based on this data, we created a Bayesian multinomial logistic regression model that quantified the correlations between landscape covariates and coat colour phenology and analysed among season and year moult timing variation. Our results demonstrate that mountain hare moult timing is strongly correlated with altitude and latitude with hares that live at higher latitudes and altitudes keeping their winter white coats for longer than their conspecifics that inhabit lower latitudes and altitudes. Moult timing was also weakly correlated with climate zone with hares that live in coastal climates keeping their winter white coats for longer than hares that live in continental climates. We found evidence of some among year moult timing variation in spring, but not in autumn. We conclude that mountain hare moult timing has adapted to local environmental conditions throughout Norway.
Cloud migration framework clustering method for social decision support in modernizing the legacy system
Authors: Aslam Mubeen , Rahim Lukman A. B. , Watada Junzo , Rubab Saddaf , Khan Muhammad Attique , AlQahtani Salman A. , Gadekallu Thippa Reddy 
Published: October 2023

AbstractCloud solutions accelerate the large‐scale acceptance of IoT projects. By diminishing the need for maintaining on‐premises infrastructure, the cloud has enabled corporations to surpass the traditional applications of IoT (e.g., in‐home appliances) and opened the doors for large‐scale deployment of IoT applications on the cloud. However, shifting legacy systems to the cloud environment can be considerably difficult. Accordingly, this article proposes a method that may support organizations in deciding to modernize their legacy systems. The main concept of this study is to discuss the modernization strategies in detail and to support organizations in selecting the most accurate and appropriate cloud migration strategy, based on their requirements of the legacy system. This article introduces a novel research process, called the K‐means cosine cloud clustering method (K3CM). K3CM is a statistical knowledge‐based method for identifying and clustering the most relevant and similar cloud migration strategies. The quality of a cluster is evaluated by measuring intra‐cohesiveness. Simulation experiments statistically analyzed, evaluated, and verified the quality of K3CM clusters. Correspondence analysis explored the similarity and relationship among cloud migration frameworks and validated the proposed technique. The statistical and simulation results of this study focus on the analytics and decision support system implementation that provides a reliable, valid, and robust clustering method for modernizing the legacy system.
iso‐Fatty Acid Metabolism in Caenorhabditis elegans’ Ceramide Biosynthesis
Authors: Rivera Sánchez Rocío , Bandi Siva , Scheidt Marie‐Désirée , Laaroussi Hanna , William Fox Bennett , Ishida Yojiro , Glauser Gaétan , Sutour Sylvain , von Reuss Stephan H. 
Published: October 2023

AbstractCeramide biosynthesis and its connection to iso‐fatty acid metabolism in the model organism Caenorhabditis elegans was investigated using a combination of reverse genetics and comparative ESI‐(+)‐HR‐MSe ceramide profiling along with incorporation experiments with bacterial mutants specifically enriched with isotopically labeled branched‐chain amino acids or branched‐chain fatty acids. Incorporation of a l‐leucine‐derived isovalerate unit into the conserved d17 : 1iso sphingosine building block proceeds through elo‐5 dependent chain elongation and depends on peroxisomal β‐oxidation by the 3‐ketoacyl‐CoA thiolase daf‐22, although ceramide profiles of N2 wildtype and daf‐22(ok693) are indistinguishable. Biosynthesis of the homologous N‐iso‐acyl moieties also depends on l‐leucine and isovalerate chain elongation but proceeds independently of elo‐5 and daf‐22. Biosynthesis of the dominating N‐docosanoyl moiety depends on elo‐3‐catalyzed chain elongation of bacteria‐derived palmitic acid, whereas the N‐tetracosanoyl moiety is derived from de novo lipogenesis.
Association of Medicaid expansion with 2‐year survival and time to treatment initiation in gastrointestinal cancer patients: A National Cancer Database study
Authors: Mobley Erin M. , Chen Guanming , Xu Jie , Edgar Lauren , Pather Keouna , Daly Meghan C. , Awad Ziad T. , Parker Alexander S. , Xie Zhigang , Suk Ryan , Mathews Simon , Hong Young‐Rock 
Published: October 2023

AbstractIntroductionWe evaluated whether Medicaid expansion (ME) was associated with improved 2‐year survival and time to treatment initiation (TTI) among patients with gastrointestinal (GI) cancer.MethodsGI cancer patients diagnosed 40–64 years were queried from the National Cancer Database. Those diagnosed from 2010 to 2012 were considered pre‐expansion; those diagnosed from 2014 to 2016 were considered post‐expansion. Cox models estimated hazard ratios and 95% confidence intervals (CIs) for 2‐year overall survival. Generalized estimating equations (GEE) estimated odds ratios (OR) and 95% CI of TTI within 30‐ and 90 days. Multivariable Difference‐in‐Difference models were used to compare expansion/nonexpansion cohorts pre‐/post‐expansion, adjusting for patient, clinical, and hospital factors.Results377,063 patients were included. No significant difference in 2‐year survival was demonstrated across ME and non‐ME states overall or in site‐based subgroup analysis. In stage‐based subgroup analysis, 2‐year survival significantly improved among stage II cancer, with an 8% decreased hazard of death at 2 years (0.92; 0.87–0.97). Those with stage IV had a 4% increased hazard of death at 2 years (1.04; 1.01–1.07). Multivariable GEE models showed increased TTI within 30 days (1.12; 1.09–1.16) and 90 days (1.22; 1.17–1.27). Site‐based subgroup analyses indicated increased likelihood of TTI within 30 and 90 days among colon, liver, pancreas, rectum, and stomach cancers, by 30 days for small intestinal cancer, and by 90 days for esophageal cancer. In subgroup analyses, all stages experienced improved odds of TTI within 30 and 90 days.ConclusionME was not associated with significant improvement in 2‐year survival for those with GI cancer. Although TTI increased after ME for both cohorts, the 30‐ and 90‐day odds of TTI was higher for those from ME compared with non‐ME states. Our findings add to growing evidence of associations with ME for those diagnosed with GI cancer.
Disaster exposure and mental health among Puerto Rican teachers after Hurricane Maria
Authors: Sackey Enoch T. , Stewart Regan W. , Young John , Orengo‐Aguayo Rosaura 
Published: October 2023

AbstractThe purpose of the study was to assess the prevalence rates of anxiety, depression, and posttraumatic stress disorder (PTSD) outcomes among teachers (N = 8,167) exposed to Hurricane Maria in Puerto Rico. The findings indicate that the rates of likely significant symptoms of anxiety, depression, and PTSD were 13.1%, 8.7%, and 5.4%, respectively. There were no significant disparities in psychopathological symptoms based on sex, except that female participants were more likely to exhibit anxiety symptoms than their male counterparts, odds ratio (OR) = 1.53. Key disaster‐related factors, such as perceived threat, actual threat, and disruption of life were found to significantly influence all psychopathology outcomes, ORs = 1.20–3.57, whereas increased social support significantly buffered the manifestation of clinically significant psychopathology symptoms across all domains, ORs = 0.30–0.46. These outcomes, strongly linked to peritraumatic consequences, were not constrained by geographical or socioeconomic boundaries, highlighting the wide‐ranging impact of the hurricane. Nevertheless, social support emerged as a significant counteracting force, reducing the likelihood of detrimental mental health symptoms. Consistent with prior studies, this study emphasizes the crucial role of social support in nurturing posttraumatic resilience and curbing the severity of posttraumatic stress outcomes, thus underscoring the need for incorporating robust social support systems within disaster response strategies.
Long‐term clinical outcomes of Spitz‐type lesions in adults: A single institution retrospective cohort review
Authors: Chintapalli Renuka , Chintapalli Prakash , Patel Animesh 
Published: October 2023

AbstractBackgroundThe lack of information on clinical outcomes of Spitz‐type lesions, means that their management remains a contentious issue, with some centres opting for a purely conservative, observational approach, while others mandate further surgery and long‐term follow‐up.ObjectivesOur aim was, therefore, to determine the long‐term clinical outcome of classic Spitz and Spitzoid naevi, atypical Spitz tumours (ASTs) and Spitzoid melanomas, with a view to finding evidence to support a unified management approach.MethodsWe conducted a retrospective cohort review of Addenbrooke's Hospital patients aged ≥18 years with a histopathologic diagnosis containing the term ‘Spitz’, from 1991 through 2020. Sentinel lymph node biopsy (SLNB) results, wide local excision (WLE) rates, metastases and fatality were assessed.ResultsOne hundred and twenty‐six patients with Spitz‐type proliferations and follow‐up information were identified (mean age: 35.9, SD: 11.6). There were 86 (68.3%) classic Spitz or Spitzoid naevi, 12 (9.5%) ASTs, 27 (21.4%) Spitzoid malignant melanomas and one (0.8%) melanoma that arose in a previously excised Spitz naevus. SLNB findings were positive in three of seven but none of the 10 individuals with ASTs and Spitzoid melanomas, respectively. Invasive non‐Spitz melanoma developed in four patients with classic Spitz or Spitzoid naevi. After a median follow‐up of 46 months (range: 1–304), only one patient with a Spitzoid melanoma, who had a different malignant melanoma (2.4 mm, level 4), developed distant metastases. No deaths were observed.ConclusionsSpitz‐type lesions are only rarely associated with mortality and distant metastasis, but CSNs may increase the risk of developing non‐Spitz‐type malignant melanoma. These findings support the suggestion that aggressive surgical management of these lesions such as SLNB and WLE may not be necessary, but surveillance should still continue to assess for subsequent development of malignant melanoma.
Photocatalytic Applications of Polypyrrole/Polythiophene Co‐Oligomers for the Degradation of Bisphenol A
Authors: Farooq Aaliyah , Alam Javed , Riaz Ufana 
Published: October 2023

AbstractWith a view to explore the photocatalytic effect of conjugated oligomers against pollutants, the present work reports ultrasound‐assisted polymerization of pyrrole, thiophene and their oligomers in mol ratios of 80/20, 50/50, and 20/80. The synthesized oligomers were characterized using FTIR, UV‐Vis, and SEM. The co‐oligomers showed band gap values ranging between 2.6 eV–2.9 eV, higher than pristine polypyrrole (PPy) and polythiophene (PTh). The photocatalytic degradation of Bisphenol A was carried out under UV irradiation. Maximum degradation of 86 % within a span of 120 min was reported using PPy/PTh‐80/20 as photocatalyst. A tentative degradation pathway and mechanism of degradation were proposed based on the LCMS data and radical scavenging studies. The studies showed good photocatalytic properties of organic photocatalysts as compared to inorganic metal oxide‐based photocatalysts in terms of higher degradation efficiency attained in a short span of 120 min even for higher concentrations of BPA.
Fabrication of MIL‐101(Fe) on UiO‐66 as an Electrocatalyst for Enhanced Overall Water Splitting
Authors: Ali Maryum , Pervaiz Erum 
Published: October 2023

AbstractThe water‐splitting process is an attractive avenue for manufacturing hydrogen gas (a green and renewable energy source). However, this process requires efficient and economical electrocatalysts to lessen the high energy barrier of water oxidation and hydrogen evolution reactions. MOF‐on‐MOF hybrid structures assembled by unifying two or more MOF units are being explored as electrocatalysts with promising results. In this study, a novel electrocatalyst nanostructure of an iron‐based MOF on a zirconium‐based MOF denoted as MIL‐101(Fe)/UiO‐66 was prepared by hydrothermal synthesis. MIL‐101(Fe) offers abundant active sites with higher surface area for catalytic activity, while UiO‐66 acts as a support to enhance stability and conductivity. It was observed that compared to the individual MOFs, hybrid (MIL‐101(Fe)/UiO‐66) exhibited significantly enhanced catalytic efficiency towards overall water‐splitting. This was evidenced by the higher current densities and lower overpotentials observed for the hybrid. The MIL‐101(Fe)/UiO‐66 showed an overpotential of 185 mV for HER and 290 mV for OER @ η10 in an alkaline electrolyte solution. Moreover, it was stable for over 24 hours with a very small decrease in activity. With MIL‐101(Fe)/UiO‐66 as the cathode and anode, the unified water‐splitting cell attained a low overall required potential of 1.58 V @ η10 demonstrating good performance of overall water splitting. It offers an appealing strategy for creating high‐performance electrocatalysts for energy‐related applications, in particular water splitting, by combining the advantages of different MOFs through facile fabrication methods.
Characterizing Nickel Oxide Thin Films for Smart Window Energy Conversion Applications: Combined Experimental and Theoretical Analyses
Authors: Khera Ejaz Ahmad , Ullah Hafeez , Hussain Fayyaz , Abubakar Shahzad , Majeed Abdul , Tabssum Iqra , Batool Zahida , Nazir Aalia , Gilanie Ghulam 
Published: October 2023

AbstractIn this work, we have analyzed key characteristics of nickel oxide (NiO) thin films using combined experimental and computational approaches for smart window applications. Thermo Gravimetric Analysis (TGA) depicts that nickel oxide films are stable beyond 275 °C.The XRD pattern revealed the single phase of the materials. FTIR studies confirm the stretching vibrational band of Ni−O. The current‐voltage analysis shows the semiconducting behavior of the materials. The synthesized NiO thin films have shown prominent electrochromic response with a wide linear range, better cyclic stability, high durability, and fast response time. The sample having concentration of 0.05 M/25 mL has best semi‐conductive response as rapid increase in current occurs above 0.8 V. Moreover, the electrochemical and optical properties have shown reversible redox behavior of the synthesized NiO thin films with changing their appearance from original greenish gray to the dark brown in the selected potential range. In addition, the computational study has also been performed for deep study of electronic charge conduction mechanism and photo response of NiO thin layer using Tran‐Blaha modified Becke Johnson (TB‐mBJ) approach. These fabricated NiO thin films have been utilized as an active electrode for electro chromic devices in smart window applications.
Ratiometric analysis of in vivo optical coherence tomography retinal layer thicknesses for detection of changes in Alzheimer's disease
Authors: Sharma Shonit N. , Marsh Jordan W. , Tsipursky Michael S. , Boppart Stephen A. 
Published: October 2023

AbstractWe analyzed ophthalmic retinal optical coherence tomography (OCT) images from patients with Alzheimer's disease (AD) to identify retinal layer thickness and ratio changes that may serve as image‐based biomarkers for the disease. One three‐dimensional volume before and one after diagnosis for each of 48 patients were segmented to identify retinal layer and total retinal thicknesses. Between before‐ and after‐diagnosis retinal OCT images, there were significant thickness changes in six of 10 (60%) retinal layers across all 48 patients. Through a comparison with age‐matched healthy subjects, the significant changes were attributed to AD only (NFL and PR2 layers), age only (GCL, IPL, and RPE layers), or both AD and age (OPL layer). Analyzing ratios of retinal layer thicknesses, 53 of 90 (58.89%) ratios had significant changes. The four independently nonsignificant layers were assessed to be affected by neither AD nor age (INL layer) or both AD and age (ELM, PR1, and BM layers). The demonstrated image segmentation, measurement, and ratiometric analysis of retinal layers in AD patients may yield a noninvasive OCT image‐based retinal biomarker that can be used to detect retinal changes associated with this disease.
Family with sequence similarity 111 member B contributes to tumor growth and metastasis by mediating cell proliferation, invasion, and EMT via transforming acidic coiled‐coil protein 3/PI3K/AKT signaling pathway in hepatocellular carcinoma
Authors: Yang Yaobo , Yan Zhaoyong , Jiao Yang , Yang Weihao , Cui Qi , Chen Sipan 
Published: October 2023

AbstractAs a complex systemic disease, primary liver cancer ranks third in death rate for solid tumors worldwide. Family with sequence similarity 111 member B (FAM111B), which was found to be aberrantly mutated in multiple cancers, is a candidate oncogene. We aimed to determine the function and mechanism of FAM111B in hepatocellular carcinoma (HCC). The expression of FAM111B was evaluated in HCC tissues, adjacent tissues, HCC cell lines. The impact of FAM111B on proliferation, invasion, apoptosis and EMT of HCC cells were detected by CCK‐8, Transwell, flow cytometry and Western blot assays. The relationship between FAM111B and transforming acidic coiled‐coil protein 3 (TACC3) was assessed by CoIP and Immunofluorescence (IF) staining assays. The effect of FAM111B on tumor growth was detected by using xenograft model of nude mice. The expression of FAM111B was upregulated in HCC tissues and cell lines, and the prognosis of HCC patients was worse in the high FAM111B expression group, and its expression level was associated with the TNM stage of HCC. FAM111B silencing inhibited HCC cell proliferation and invasion, EMT and induced apoptosis. Besides, TACC3 served as an interactor for FAM111B, which could enhance TACC3 expression, thus activing PI3K/AKT pathway. Rescue experiments revealed that elevated of TACC3 restored the inhibitory effect of FAM111B overexpression on the cell functions via PI3K/AKT pathway. In vivo, FAM111B inhibition hampered tumor growth and metastasis of HCC. This study highlighted a key player of FAM111B in modulating the malignant biological progression of HCC via TACC3/PI3K/AKT signaling pathway, displaying a potential therapeutic target for HCC.