bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time-lapse confocal microscopy to study in vitro Streptococcus mutans surface colonization
Authors: Wing Jason T F , Hayashi Michael A L , Redissi Aneesa F , Vickerman M Margaret , Tenuta Livia M A , Fenno J Christopher , Rickard Alexander H 
Published: February 2024

Abstract
The cariogenicity of Streptococcus mutans relates to its ability to form biofilms on dental surfaces. The aim of this work was to develop a flowcell system compatible with time-lapse confocal microscopy to compare the adhesion and accumulation of S. mutans cells on surfaces in unsupplemented media against media containing sucrose or sucralose (a non-metabolized sweetener) over a short period of time. Fluorescent S. mutans 3209/pVMCherry was suspended in unsupplemented media or media supplemented with 1% sucrose or 1% sucralose and passed through a 3D-printed flowcell system. Flowcells were imaged over 60 minutes using a confocal microscope. Image analysis was performed, including a newly developed object-movement-based method to measure biomass adhesion. Streptococcus mutans 3209/pVMCherry grown in 1% sucrose-supplemented media formed small, dense, relatively immobile clumps in the flowcell system measured by biovolume, surface area, and median object centroid movement. Sucralose-supplemented and un-supplemented media yielded large, loose, mobile aggregates. Architectural metrics and per-object movement were significantly different (P &lt; 0.05) when comparing sucrose-supplemented media to either unsupplemented or sucralose-supplemented media. These results demonstrate the utility of a flowcell system compatible with time-lapse confocal microscopy and image analysis when studying initial biofilm formation and adhesion under different nutritional conditions.
Light and Electron Microscopy Study of Opportunistic Free-Living Nematodes Scavenging and Thriving within Buried Dinosaur Bones
Authors: Armitage Mark H 
Published: February 2024

AbstractNematode worms are the most abundant multicellular organism on Earth. They thrive in every habitat known, and they are voracious feeders within the top 70 cm of soils. Their sturdy cuticle protects them from environmental factors and predators. Nematodes play a significant role in the decomposition of vertebrate remains in soil and serve as indicators of nutrients that enter soils during decomposition. Certain parasitic nematodes have been identified in fossil remains, but reports of fossil worms are rare. We demonstrate the abundant presence of opportunistic nematodes feeding within dinosaur bones from the Hell Creek formation, MT. The presence of visible worm ultrastructure indicates that they were alive when preserved within the dinosaur bones. Our findings are identical to worms characterized as “blood parasites” in a dinosaur bone from Brazil, demonstrating that there is sufficient soft tissue within dinosaur bone canals to sustain large populations of nematodes post-mortem.
A refined search for high-velocity gas in the Cygnus Loop supernova remnant
Authors: Ritchey Adam M , Federman S R , Lambert David L 
Published: February 2024

ABSTRACT
We present the results of a sensitive search for high-velocity gas in interstellar absorption lines associated with the Cygnus Loop supernova remnant (SNR). We examine high-resolution high signal-to-noise ratio optical spectra of six stars in the Cygnus Loop region with distances greater than ∼700 pc. All stars show low-velocity Na i and Ca ii absorption. However, only one star, HD 198301, exhibits high-velocity Ca ii absorption components, at velocities of +62, +82, and +96 km s−1. The distance to this star of ∼870 pc helps to constrain the distance to the receding edge of the Cygnus Loop’s expanding shock front. One of our targets, HD 335334, was previously thought to exhibit high positive and high negative velocity interstellar Na i and Ca ii absorption. This was one factor leading Fesen et al. to derive a distance to the Cygnus Loop of 725 ± 15 pc. However, we find that HD 335334 is in fact a double-line spectroscopic binary and shows no evidence of high-velocity interstellar absorption. As such, the distance to HD 335334 cannot be used to constrain the distance to the Cygnus Loop. Our detection of Ca ii absorption approaching 100 km s−1 toward HD 198301 is the first conclusive detection of high-velocity absorption from a low-ionization species associated with the Cygnus Loop SNR. A large jump in the Na i column density toward BD+31 4218, a star located beyond the northwestern boundary of the Cygnus Loop, helps to constrain the distance to a large molecular cloud complex with which the Cygnus Loop is evidently interacting.
Transport-driven super-Jeans fragmentation in dynamical star-forming regions
Authors: Li Guang-Xing 
Published: February 2024

ABSTRACT
The Jeans criterion is one cornerstone in our understanding of gravitational fragmentation. A critical limitation of the Jeans criterion is that the background density is assumed to be a constant, which is often not true in dynamic conditions such as star-forming regions. For example, during the formation phase of the high-density gas filaments in a molecular cloud, a density increase rate $\dot{\rho }$ implies a mass accumulation time of $t_{\rm acc}= \rho / \dot{\rho }= - \rho (\nabla \cdot (\rho \vec{v}))^{-1}$. The system is non-stationary when the mass accumulation time becomes comparable to the free-fall time $t_{\rm ff} = 1 / \sqrt{G \rho }$. We study fragmentation in non-stationary settings, and find that accretion can significantly increase in the characteristic mass of gravitational fragmentation (λJeans, aac = λJeans(1 + tff/tacc)1/3, $m_{\rm Jeans,\, acc} = m_{\rm Jeans} (1 + t_{\rm ff} / t_{\rm acc})$). In massive star-forming regions, this mechanism of transport-driven super-Jeans fragmentation can contribute to the formation of massive stars by causing order-of-magnitude increases in the mass of the fragments.
Studying the nulling, subpulse drifting, and moding in PSR J1921+1948 with the FAST
Authors: Shang Lunhua , Bai Juntao , Xu Xin , Dang Shijun , Wang Shuangqiang , Dong Aijun , Pang Lijun , Yu Yan , Li Qingying 
Published: February 2024

ABSTRACT
We reported the observations of nulling, subpulse drifting, and moding of radio radiation in pulsar (PSR) B1918+19 at 1250 MHz with the Five-hundred-meter Aperture Spherical radio Telescope. The nulling fraction is 2.6 ± 0.1 per cent and no periodicity of nulling can be seen. We confirmed the existence of three different drift modes (A, B, C) and a disordered mode (N) at 1250 MHz. Unlike the other three modes where the second component of the average pulse profile dominates, the first component of the average pulse profile in mode C is brighter than the other components, the second component shifts forward and the fourth component shifts backward. The bidrifting phenomenon is observed in PSR B1918+19, the drifting of the first and second components is positive, and the drift direction of the fourth component is negative. The drifting rate of the drift bands composed of the first and second components has an obvious evolutionary trend. Mode B has a short duration and no clear trend can be seen. But for mode A, the drift rate of each drift band starts relatively fast, then slows down to a steady state, and finally increases slowly until it enters either null or mode N. Further analysis shows that the emergence and significant variation in the drifting period of multidrifting subpulse emission modes for PSR B1918+19 may be due to the aliasing effect. The interesting subpulse emission phenomenon of PSR B1918+19 at different frequencies provides a unique opportunity to understand the switching mechanism of the multidrift mode of the pulsars.
The SAMI galaxy survey: predicting kinematic morphology with logistic regression
Authors: Vaughan Sam P , van de Sande Jesse , Fraser-McKelvie A , Croom Scott , McDermid Richard , Liquet-Weiland Benoit , Barsanti Stefania , Cortese Luca , Brough Sarah , Sweet Sarah , Bryant Julia J , Goodwin Michael , Lawrence Jon 
Published: February 2024

ABSTRACT
We use the SAMI (Sydney-AAO Multi-object Integral field spectrograph) galaxy survey to study the the kinematic morphology–density relation: the observation that the fraction of slow rotator galaxies increases towards dense environments. We build a logistic regression model to quantitatively study the dependence of kinematic morphology (whether a galaxy is a fast or slow rotator) on a wide range of parameters, without resorting to binning the data. Our model uses a combination of stellar mass, star formation rate (SFR), r-band half-light radius, and a binary variable based on whether the galaxy’s observed ellipticity (ϵ) is less than 0.4. We show that, at fixed mass, size, SFR, and ϵ, a galaxy’s local environmental surface density (log10(Σ5/Mpc−2)) gives no further information about whether a galaxy is a slow rotator, that is, the observed kinematic–morphology density relation can be entirely explained by the well-known correlations between environment and other quantities. We show how our model can be applied to different galaxy surveys to predict the fraction of slow rotators which would be observed and discuss its implications for the formation pathways of slow rotators.
Resolution of the incongruency of dipole asymmetries within various large radio surveys – implications for the Cosmological Principle
Authors: Singal Ashok K 
Published: February 2024

ABSTRACT
We investigate dipole asymmetries in four large radio surveys, each spanning more than 75 per cent of the sky. Two of them, the Very Large Array Sky Survey (VLASS) and the Rapid ASKAP Continuum Survey (RACS), have recently yielded dipoles that appear incongruent with each other as well as seem inconsistent with previous radio survey dipoles and the cosmic microwave background (CMB) dipole. Because these radio surveys have large overlaps in sky coverage, comprising hence large majority of common radio sources, one would not expect significant differences between their radio dipoles, irrespective of their underlying source of origin. We examine here in detail these radio dipoles, to ascertain the source of incongruency among them. We find the VLASS and RACS data to be containing some declination-dependent systematics, seemingly in the vicinity of the declination limit of each survey. We show that the effects of such systematics can be mitigated by restricting the declination limits of the respective survey during the dipole determination. A weighted mean of the sky coordinates of thus derived dipoles from the four radio surveys lies within 1.2σ of the CMB dipole direction. However, the amplitude appears significantly larger, 3.7 ± 0.6 times or more than the CMB dipole. This puts in doubt not only the conventional wisdom that the genesis of all these dipoles, including that of the CMB dipole, is due to the Solar peculiar motion, it also raises uncomfortable questions about the Cosmological Principle, the basis of the standard ΛCDM cosmological model.
Sardinia Radio Telescope observations of the Coma cluster
Authors: Murgia M , Govoni F , Vacca V , Loi F , Feretti L , Giovannini G , Melis A , Concu R , Carretti E , Poppi S , Valente G , Bernardi G , Bonafede A , Boschin W , Brienza M , Clarke T E , de Gasperin F , Enßlin T A , Ferrari C , Gastaldello F , Girardi M , Gregorini L , Johnston-Hollitt M , Orrù E , Parma P , Perley R A , Taylor G B , Marchegiani P 
Published: February 2024

ABSTRACT
We present deep total intensity and polarization observations of the Coma cluster at 1.4 and 6.6 GHz performed with the Sardinia Radio Telescope. By combining the single-dish 1.4 GHz data with archival Very Large Array observations, we obtain new images of the central radio halo and of the peripheral radio relic where we properly recover the brightness from the large-scale structures. At 6.6 GHz, we detect both the relic and the central part of the halo in total intensity and polarization. These are the highest frequency images available to date for these radio sources in this galaxy cluster. In the halo, we find a localized spot of polarized signal, with fractional polarization of about 45 per cent. The polarized emission possibly extends along the north-east side of the diffuse emission. The relic is highly polarized, up to 55 per cent, as usually found for these sources. We confirm the halo spectrum is curved, in agreement with previous single-dish results. The spectral index is α = 1.48 ± 0.07 at a reference frequency of 1 GHz and varies from α ≃ 1.1, at 0.1 GHz, up to α ≃ 1.8, at 10 GHz. We compare the Coma radio halo surface brightness profile at 1.4 GHz (central brightness and e-folding radius) with the same properties of the other haloes, and we find that it has one of the lowest emissivities observed so far. Reanalysing the relic’s spectrum in the light of the new data, we obtain a refined radio Mach number of M = 2.9 ± 0.1.
Effects of optogenetic silencing the anterior cingulate cortex in a delayed non-match to trajectory task
Authors: Cruz Ana S , Cruz Sara , Remondes Miguel 
Published: February 2024

Abstract
Working memory is a fundamental cognitive ability, allowing us to keep information in memory for the time needed to perform a given task. A complex neural circuit fulfills these functions, among which is the anterior cingulate cortex (CG). Functionally and anatomically connected to the medial prefrontal, retrosplenial, midcingulate and hippocampus, as well as motor cortices, CG has been implicated in retrieving appropriate information when needed to select and control appropriate behavior. The role of cingulate cortex in working memory-guided behaviors remains unclear due to the lack of studies reversibly interfering with its activity during specific epochs of working memory. We used eNpHR3.0 to silence cingulate neurons while animals perform a standard delayed non-match to trajectory task, and found that, while not causing an absolute impairment in working memory, silencing cingulate neurons during retrieval decreases the mean performance if compared to silencing during encoding. Such retrieval-associated changes are accompanied by longer delays observed when light is delivered to control animals, when compared to eNpHR3.0+ ones, consistent with an adaptive recruitment of additional cognitive resources.
Gut–liver microphysiological systems revealed potential crosstalk mechanism modulating drug metabolism
Authors: Kurniawan Dhimas Agung , Leo Sylvia , Inamatsu Mutsumi , Funaoka Sohei , Aihara Taichi , Aiko Mizuno , Rei Inoue , Sakura Takeshi , Arakawa Hiroshi , Kato Yukio , Matsugi Tomoaki , Esashika Katsuhiro , Shiraki Nobuaki , Kume Shoen , Shinha Kenta , Kimura Hiroshi , Nishikawa Masaki , Sakai Yasuyuki 
Published: February 2024

Abstract
The small intestine and liver play important role in determining oral drug's fate. Both organs are also interconnected through enterohepatic circulation, which imply there are crosstalk through circulating factors such as signaling molecules or metabolites that may affect drug metabolism. Coculture of hepatocytes and intestinal cells have shown to increase hepatic drug metabolism, yet its crosstalk mechanism is still unclear. In this study, we aim to elucidate such crosstalk by coculturing primary human hepatocytes harvested from chimeric mouse (PXB-cells) and iPSc-derived intestinal cells in a microphysiological systems (MPS). Perfusion and direct oxygenation from the MPS were chosen and confirmed to be suitable features that enhanced PXB-cells albumin secretion, cytochrome P450 (CYP) enzymes activity while also maintaining barrier integrity of iPSc-derived intestine cells. Results from RNA-sequencing showed significant upregulation in gene ontology terms related to fatty acids metabolism in PXB-cells. One of such fatty acids, arachidonic acid, enhanced several CYP enzyme activity in similar manner as coculture. From the current evidences, it is speculated that the release of bile acids from PXB-cells acted as stimuli for iPSc-derived intestine cells to release lipoprotein which was ultimately taken by PXB-cells and enhanced CYP activity.
Rails and responsibilities: Perspectives of railroad police officers in China
Authors: Xu Haoran 
Published: February 2024

Abstract
In policing practices, railway policing has an essential role to ensure the public security of extensive railway network. However, limited attention has been given to understanding the perspectives of police officers involved in this specialized field. This study aims to fill this gap by exploring the perspectives of railway police officers. Through in-depth interviews with 31 railway police officers from different regions, this research examines their experiences, challenges, and perceptions regarding their roles and responsibilities. The findings reveal that conflicts of interest within the railway police department pose potential risks to its internal cohesion. These officers used different strategies for response to violent attacks in ordinary and high-speed trains, highlighted instances of avoidance of duties due to internal conflicts, suggesting deficiencies in the evaluation mechanism employed. This study not only contributes to our understanding of railway policing in China but also provides insights for global railway policing and criminal investigation coordination.
Variation in Utilization of Physical Therapist and Occupational Therapist Services After Rotator Cuff Repair: A Population-Based Study
Authors: Stern Brocha Z , Zubizarreta Nicole , Anthony Shawn G , Gladstone James N , Poeran Jashvant 
Published: February 2024

Abstract

Objective
The objective of this study was to describe the utilization of physical therapist and occupational therapist services after rotator cuff repair (RCR) and examine variation in rehabilitation characteristics by profession.

Methods
This retrospective cohort study used the IBM MarketScan Commercial Claims and Encounters database. Eligible patients were 18 to 64 years old and had undergone outpatient RCR between 2017 and 2020. Physical therapist and occupational therapist services were identified using evaluation and treatment codes with profession-specific modifiers (“GP” or “GO”). Factors predicting utilization of formal rehabilitation and physical therapist versus occupational therapist services were examined; and univariable and multivariable analyses of days to initiate therapy, number of visits, and episode length by profession were completed.

Results
Among 53,497 patients with an RCR, 81.2% initiated formal rehabilitation (93.8% physical therapist, 5.2% occupational therapist, 1.0% both services). Patients in the Northeast and West (vs the South) were less likely to receive rehabilitation (odds ratio [OR] = 0.67 to 0.70) and less likely to receive occupational therapist services (OR = 0.39). Patients living in the Midwest (versus the South) were less likely to receive rehabilitation (OR = 0.79) but more likely to receive occupational therapist services (OR = 1.51). Similarly, those living in a rural (versus urban) area were less likely to utilize rehabilitation (OR = 0.89) but more likely to receive occupational therapist services (OR = 2.21). Additionally, receiving occupational therapist instead of physical therapist services was associated with decreased therapist visits (−16.89%), days to initiate therapy (−13.43%), and episode length (−13.78%).

Conclusion
Most patients in our commercially insured cohort utilized rehabilitation services, with a small percentage receiving occupational therapist services. We identified profession-specific variation in utilization characteristics that warrants further examination to understand predictors and associated outcomes.

Impact
Variation in rehabilitation utilization after RCR, including profession-specific and regional differences, may indicate opportunities to improve standardization and quality of care.
Connectome-based predictive modeling of Internet addiction symptomatology
Authors: Feng Qiuyang , Ren Zhiting , Wei Dongtao , Liu Cheng , Wang Xueyang , Li Xianrui , Tie Bijie , Tang Shuang , Qiu Jiang 
Published: February 2024

Abstract
Internet addiction symptomatology (IAS) is characterized by persistent and involuntary patterns of compulsive Internet use, leading to significant impairments in both physical and mental well-being. Here, a connectome-based predictive modeling approach was applied to decode IAS from whole-brain resting-state functional connectivity in healthy population. The findings showed that IAS could be predicted by the functional connectivity between prefrontal cortex with the cerebellum and limbic lobe and connections of the occipital lobe with the limbic lobe and insula lobe. The identified edges associated with IAS exhibit generalizability in predicting IAS within an independent sample. Furthermore, we found that the unique contributing network, which predicted IAS in contrast to the prediction networks of alcohol use disorder symptomatology (the range of symptoms and behaviors associated with alcohol use disorder), prominently comprised connections involving the occipital lobe and other lobes. The current data-driven approach provides the first evidence of the predictive brain features of IAS based on the organization of intrinsic brain networks, thus advancing our understanding of the neurobiological basis of Internet addiction disorder (IAD) susceptibility, and may have implications for the timely intervention of people potentially at risk of IAD.
Induction of lung progenitor cell‐like organoids by porcine pluripotent stem cells
Authors: Yang Xinchun , Wu Xiaolong , Wang Yuqi , Li Wenhao , Wu Xiaojie , Yuan Liming , Yu Taiyong , Li Na , Zhang Shiqiang , Hua Jinlian 
Published: February 2024

AbstractOrganoids are in vitro 3D models that are generated using stem cells to study organ development and regeneration. Despite the extensive research on lung organoids, there is limited information on pig lung cell generation or development. Here, we identified five epithelial cell types along with their characteristic markers using scRNA‐seq. Additionally, we found that NKX2.1 and FOXA2 acted as the crucial core transcription factors in porcine lung development. The presence of SOX9/SOX2 double‐positive cells was identified as a key marker for lung progenitor cells. The Monocle algorithm was used to create a pseudo‐temporal differentiation trajectory of epithelial cells, leading to the identification of signaling pathways related to porcine lung development. Moreover, we established the differentiation method from porcine pluripotent stem cells (pPSCs) to SOX17+FOXA2+ definitive endoderm (DE) and NKX2.1+FOXA2+CDX2− anterior foregut endoderm (AFE). The AFE is further differentiated into lung organoids while closely monitoring the differentiation process. We showed that NKX2.1 overexpression facilitated the induction of lung organoids and supported subsequent lung differentiation and maturation. This model offers valuable insights into studying the interaction patterns between cells and the signaling pathways during the development of the porcine lung.