Peripheral Transcriptomics in Acute and Long-Term Kidney Dysfunction in SARS-CoV2 Infection
Authors: Jayaraman Pushkala , Rajagopal Madhumitha , Paranjpe Ishan , Liharska Lora , Suarez-Farinas Mayte , Thompson Ryan , Del Valle Diane Marie , Beckmann Noam , Oh Wonsuk , Gulamali Faris F. , Kauffman Justin , Gonzalez-Kozlova Edgar , Dellepiane Sergio , Vasquez-Rios George , Vaid Akhil , Jiang Joy , Chen Annie , Sakhuja Ankit , Chen Steven , Kenigsberg Ephraim , He John Cijiang , Coca Steven G , Chan Lili , Schadt Eric , Merad Miram , Kim-Schulze Seunghee , Gnjatic Sacha , Tsalik Ephraim , Langley Raymond , Charney Alexander W , Nadkarni Girish N 
Published: October 2023

ABSTRACTBackgroundAcute kidney injury (AKI) is common in hospitalized patients with SARS-CoV2 infection despite vaccination and leads to long-term kidney dysfunction. However, peripheral blood molecular signatures in AKI from COVID-19 and their association with long-term kidney dysfunction are yet unexplored.MethodsIn patients hospitalized with SARS-CoV2, we performed bulk RNA sequencing using peripheral blood mononuclear cells(PBMCs). We applied linear models accounting for technical and biological variability on RNA-Seq data accounting for false discovery rate (FDR) and compared functional enrichment and pathway results to a historical sepsis-AKI cohort. Finally, we evaluated the association of these signatures with long-term trends in kidney function.ResultsOf 283 patients, 106 had AKI. After adjustment for sex, age, mechanical ventilation, and chronic kidney disease (CKD), we identified 2635 significant differential gene expressions at FDR<0.05. Top canonical pathways wereEIF2signaling, oxidative phosphorylation, mTOR signaling, and Th17 signaling, indicating mitochondrial dysfunction and endoplasmic reticulum (ER) stress. Comparison with sepsis associated AKI showed considerable overlap of key pathways (48.14%). Using follow-up estimated glomerular filtration rate (eGFR) measurements from 115 patients, we identified 164/2635 (6.2%) of the significantly differentiated genes associated with overall decrease in long-term kidney function. The strongest associations were ‘autophagy’, ‘renal impairment via fibrosis’, and ‘cardiac structure and function’.ConclusionsWe show that AKI in SARS-CoV2 is a multifactorial process with mitochondrial dysfunction driven by ER stress whereas long-term kidney function decline is associated with cardiac structure and function and immune dysregulation. Functional overlap with sepsis-AKI also highlights common signatures, indicating generalizability in therapeutic approaches.SIGNIFICANCE STATEMENTPeripheral transcriptomic findings in acute and long-term kidney dysfunction after hospitalization for SARS-CoV2 infection are unclear. We evaluated peripheral blood molecular signatures in AKI from COVID-19 (COVID-AKI) and their association with long-term kidney dysfunction using the largest hospitalized cohort with transcriptomic data. Analysis of 283 hospitalized patients of whom 37% had AKI, highlighted the contribution of mitochondrial dysfunction driven by endoplasmic reticulum stress in the acute stages. Subsequently, long-term kidney function decline exhibits significant associations with markers of cardiac structure and function and immune mediated dysregulation. There were similar biomolecular signatures in other inflammatory states, such as sepsis. This enhances the potential for repurposing and generalizability in therapeutic approaches.