bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of the new video laryngeal mask airway SaCoVLM on airway management in lateral laparoscopic urological surgery: A single center randomized controlled trial
Authors: Sun Yongtao , Zhang Min , Gao Xiaojun , Gao Zhongquan , Zou Ting , Guo Yongle , Liu Mengjie , Chen Lina , Zhang Xiaoning , Liu Yang , Feng Hai , Wang Yuelan 
Published: January 2024

AbstractThere are few pertinent studies about the application of laryngeal mask airways (LMAs) in lateral decubitus surgery. Therefore, the aim of our study was to evaluate the effects of lateral position and pneumoperitoneum on oropharyngeal leak pressure (OLP) and ventilation efficiency for the LMA SaCoVLM. Patients undergoing elective retroperitoneal laparoscopic urological surgery were randomized 1:1 to the Supreme group or SaCoVLM group. The primary outcome was the OLP with LMA insertion. The secondary outcomes were the first-attempt success rate, insertion time, adjustment times, gastric tube success rate, LMA alignment accuracy, LMA removal time, regurgitation or aspiration, LMA blood staining, and incidence of adverse events 24 h after surgery. We recruited 70 patients to complete the study. Regardless of lateral position and pneumoperitoneum, the OLP was greater in the SaCoVLM group (n = 35) than in the Supreme group (n = 35), with a median difference of 4–7 cmH2O. The first-attempt success rate of the SaCoVLM group was higher than that of the Supreme group (91.4% vs. 77.1%, risk ratio (RR): 1.19; 95% CI 0.96 to 1.46, P = 0.188). Thus, in the lateral position with pneumoperitoneum, although the new video LMA SaCoVLM has a higher OLP than the LMA Supreme, both devices provide sufficient ventilation efficiency.
Linking drought indices to atmospheric circulation in Svalbard, in the Atlantic sector of the High Arctic
Authors: Migała Krzysztof , Łupikasza Ewa , Osuch Marzena , Opała-Owczarek Magdalena , Owczarek Piotr 
Published: January 2024

AbstractBased on long-term climatological data from Ny-Ålesund, Svalbard Airport—Longyearbyen and the Polish Polar Station at Hornsund, we undertook an analysis of drought indices on Spitsbergen Island, Svalbard, for the period 1979–2019. The features and causes of spatiotemporal variability of atmospheric drought in Svalbard were identified, as expressed by the standardized precipitation evapotranspiration index (SPEI). There were several-year periods with SPEI indicating the dominance of drought or wet conditions. The long-term variability in the annual and half-year (May–October) SPEI values showed a prevalence of droughts in the 1980s and the first decade of the twenty-first century, while wet seasons were frequent in the 1990s and in the second decade of the twenty-first century. The seasonal SPEIs were characteristic of interannual variability. In MAM and JJA, droughts were more frequent after 2000; during SON and DJF of the same period, the frequency of wet seasons increased. The most remarkable changes in the scale of the entire research period occurred in autumn when negative values of SPEI occurred more often in the first part of the period, and positive values dominated in the last 20 years. The long-term pattern of the variables in consecutive seasons between 1979 and 2019 indicates relationships between the SPEI and anomalies of precipitable water and somewhat weaker relationships with anomalies of sea level pressure. The three stations are located at distances of more than 200 km from each other in the northern (Ny-Ålesund), central (Longyearbyen) and southern parts of Svalbard (Hornsund), and the most extreme values of drought conditions depended on the atmospheric circulation which could have been modified by local conditions thus droughts developed under various circulation types depending on the station. However, some similarities were identified in the atmospheric circulation patterns favouring drought conditions at Ny-Ålesund and Hornsund, both having more maritime climates than Longyearbyen. Extremely dry seasons were favoured by anticyclonic conditions, particularly a high-pressure ridge (type Ka) centred over Svalbard, air advection from the eastern sector under an influence of cyclone and negative precipitable water anomalies. During wet seasons anomalies of precipitable water were positive and cyclonic conditions dominated. These results were corroborated by the frequency of regional circulation types during JJA and DJF with the lowest and highest values of SPEI.
A metabolomics perspective on 2 years of high-intensity training in horses
Authors: Johansson L. , Ringmark S. , Bergquist J. , Skiöldebrand E. , Jansson A. 
Published: January 2024

AbstractThe plasma metabolomic profile of elite harness horses subjected to different training programmes was explored. All horses had the same training programme from 1.5 until 2 years of age and then high-intensity training was introduced, with horses divided into high and low training groups. Morning blood samples were collected at 1.5, 2, 2.5 and 3.5 years of age. The plasma was analysed using targeted absolute quantitative analysis and a combination of tandem mass spectrometry, flow-injection analysis and liquid chromatography. Differences between the two training groups were observed at 2 years of age, when 161 metabolites and sums and ratios were lower (e.g. ceramide and several triglycerides) and 51 were higher (e.g. aconitic acid, anserine, sum of PUFA cholesteryl esters and solely ketogenic AAs) in High compared with low horses. The metabolites aconitic acid, anserine, leucine, HArg synthesis and sum of solely ketogenic AAs increased over time, while beta alanine synthesis, ceramides and indole decreased. Therefore high-intensity training promoted adaptations linked to aerobic energy production and amino acid metabolism, and potentially also affected pH-buffering and vascular and insulin responses.
Single cell transcriptomic representation of social dominance in prefrontal cortex and the influence of preweaning maternal and postweaning social environment
Authors: Lopez Katherine , Baker Madelyn R. , Toth Miklos 
Published: January 2024

AbstractSocial dominance encompasses winning dyadic contests and gaining priority access to resources and reproduction. Dominance is influenced by environmental factors, particularly during early postnatal life and adolescence. A disinhibitory medial prefrontal cortex (mPFC) microcircuit has been implicated in the expression of dominance in the “tube test” social competition paradigm in mice, but the neuroplasticity underlying dominance is not known. We previously reported that male pups raised by physically active (wheel-running, as opposed to sedentary) dams exhibit tube test dominance and increased reproductive fitness, and here we show that social isolation from weaning also increases dominance. By using single cell transcriptomics, we tested if increased dominance in these models is associated with a specific transcriptional profile in one or more cell-types in the mPFC. The preweaning maternal effect, but not postweaning social isolation, caused gene expression changes in pyramidal neurons. However, both the effect of maternal exercise and social isolation induced the coordinated downregulation of synaptic channel, receptor, and adhesion genes in parvalbumin positive (PV) interneurons, suggesting that development of dominance is accompanied by impaired PV interneuron-mediated inhibition of pyramidal cells. This study may help understand environmentally induced transcriptional plasticity in the PFC and its relationship to tube test dominance.
Integrative analysis of gene expression profiles of substantia nigra identifies potential diagnosis biomarkers in Parkinson's disease
Authors: Huang Junming , Li Bowen , Wei Huangwei , Li Chengxin , Liu Chao , Mi Hua , Chen Shaohua 
Published: January 2024

AbstractParkinson's disease (PD) is a progressive neurodegenerative disease whose etiology is attributed to development of Lewy bodies and degeneration of dopaminergic neurons in the substantia nigra (SN). Currently, there are no definitive diagnostic indicators for PD. In this study, we aimed to identify potential diagnostic biomarkers for PD and analyzed the impact of immune cell infiltrations on disease pathogenesis. The PD expression profile data for human SN tissue, GSE7621, GSE20141, GSE20159, GSE20163 and GSE20164 were downloaded from the Gene Expression Omnibus (GEO) database for use in the training model. After normalization and merging, we identified differentially expressed genes (DEGs) using the Robust rank aggregation (RRA) analysis. Simultaneously, DEGs after batch correction were identified. Gene interactions were determined through venn Diagram analysis. Functional analyses and protein–protein interaction (PPI) networks were used to the identify hub genes, which were visualized through Cytoscape. A Lasso Cox regression model was employed to identify the potential diagnostic genes. The GSE20292 dataset was used for validation. The proportion of infiltrating immune cells in the samples were determined via the CIBERSORT method. Sixty-two DEGs were screened in this study. They were found to be enriched in nerve conduction, dopamine (DA) metabolism, and DA biosynthesis Gene Ontology (GO) terms. The PPI network and Lasso Cox regression analysis revealed seven potential diagnostic genes, namely SLC18A2, TAC1, PCDH8, KIAA0319, PDE6H, AXIN1, and AGTR1, were subsequently validated in peripheral blood samples obtained from healthy control (HC) and PD patients, as well as in the GSE20292 dataset. The results revealed the exceptional sensitivity and specificity of these genes in PD diagnosis and monitoring. Moreover, PD patients exhibited a higher number of plasma cells, compared to HC individuals. The SLC18A2, TAC1, PCDH8, KIAA0319, PDE6H, AXIN1, and AGTR1 are potential diagnostic biomarkers for PD. Our findings also reveal the essential roles of immune cell infiltration in both disease onset and trajectory.
Effect of pharmacological selectivity of SGLT2 inhibitors on cardiovascular outcomes in patients with type 2 diabetes: a meta-analysis
Authors: Sayour Alex Ali , Oláh Attila , Ruppert Mihály , Barta Bálint András , Merkely Béla , Radovits Tamás 
Published: January 2024

AbstractSodium–glucose cotransporter 2 (SGLT2) inhibitors reduce major adverse cardiovascular events (MACE) in type 2 diabetic (T2DM) patients. Pharmacological selectivity of these agents to SGLT2 over SGLT1 is highly variant, with unknown clinical relevance. Genetically reduced SGLT1—but not SGLT2—activity correlates with lower risk of heart failure and mortality, therefore additional non-selective SGLT1 inhibition might be beneficial. In this prespecified meta-analysis, we included 6 randomized, placebo-controlled cardiovascular outcome trials of SGLT2 inhibitors assessing MACE in 57,553 patients with T2DM. Mixed-effects meta-regression revealed that pharmacological selectivity of SGLT2 inhibitors (either as continuous or dichotomized variable) had no significant impact on most outcomes. However, lower SGLT2 selectivity correlated with significantly lower risk of stroke (pseudo-R2 = 78%; p = 0.011). Indeed, dual SGLT1/2 inhibitors significantly reduced the risk of stroke (hazard ratio [HR], 0.78; 95% confidence interval [CI], 0.64–0.94), unlike selective agents (p for interaction = 0.018). The risk of diabetic ketoacidosis and genital infections was higher in both pharmacological groups versus placebo. However, hypotension occurred more often with non-selective SGLT2 inhibitors (odds ratio [OR], 1.87; 95% CI, 1.20–2.92) compared with selective agents (p for interaction = 0.044). In conclusion, dual SGLT1/2 inhibition reduces stroke in high-risk T2DM patients but has limited additional effect on other clinical outcomes.
Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits
Authors: Penteado Renato Ferras , da Silva Renata Santana , Moura Danielle Maria Nascimento , de Lima Gustavo Barbosa , Malvezzi Amaranta Muniz , Monteiro Tallyta Tâmara da Silva , Xavier Camila Cavalcanti , Vichier-Guerre Sophie , Dugué Laurence , Pochet Sylvie , Zanchin Nilson Ivo Tonin , Reis Christian Robson de Souza , de Melo Neto Osvaldo Pompílio , Guimarães Beatriz Gomes 
Published: January 2024

AbstractRecognition of the mRNA 5′ end is a critical step needed for translation initiation. This step is performed by the cap binding protein eIF4E, which joins the larger eIF4G subunit to form the eIF4F complex. Trypanosomatids have a minimum of five different eIF4F-like complexes formed through specific but not well-defined interactions between four different eIF4E and five eIF4G homologues. The EIF4E6/EIF4G5 complex has been linked with the stage-specific translation of mRNAs encoding the major Trypanosoma brucei virulence factors. Here, to better define the molecular basis for the TbEIF4E6/TbEIF4G5 interaction, we describe the identification of the peptide interacting with TbEIF4E6 in the region comprising residues 79–166 of TbEIF4G5. The TbEIF4E6-TbEIF4G5_79-116 complex reconstituted with recombinant proteins is highly stable even in the absence of cap-4. The crystal structure of the complex was subsequently solved, revealing extensive interacting surfaces. Comparative analyses highlight the conservation of the overall structural arrangement of different eIF4E/eIF4G complexes. However, highly different interacting surfaces are formed with distinct binding contacts occurring both in the canonical and noncanonical elements within eIF4G and the respective eIF4E counterpart. These specific pairs of complementary interacting surfaces are likely responsible for the selective association needed for the formation of distinct eIF4F complexes in trypanosomatids.
Facilitation and competition deconstructed: a mechanistic modelling approach to the stress gradient hypothesis applied to drylands
Authors: Díaz-Sierra Rubén , Rietkerk Max , Verwijmeren Mart , Baudena Mara 
Published: January 2024

AbstractFacilitative interactions among species are key in plant communities. While experimental tests support the Stress Gradient Hypothesis (SGH) as an association between facilitation and stress, whether the shape of net effects along stress gradients can be predicted is controversial, with no available mathematical modelling approaches. We proposed a novel test, using a modification of the R* model to study how negative and positive partial effects of plant interactions in drylands combine along two common stress gradients. We modelled different interactions: competition for water and light, amelioration of soil infiltration and/or grazing protection, obtaining that intensity and importance of facilitation did not generally increase along stress gradients, being dependent on the interaction type. While along the water stress gradient net interactions became more positive, reaching a maximum and then waning again, various outcomes were observed along the grazing gradient. Shape variety was mainly driven by the various shapes of the partial positive effects. Under resource stress, additive interaction effects can be expected, whereas when including grazing, the effects were non-additive. In the context of the SGH, deconstructing the effect of positive and negative interaction in a pairwise mechanistic models of drylands does not show a unique shape along stress gradients.
Single-shot quantitative phase-fluorescence imaging using cross-grating wavefront microscopy
Authors: Marthy Baptiste , Bénéfice Maëlle , Baffou Guillaume 
Published: January 2024

AbstractThe article introduces an optical microscopy technique capable of simultaneously acquiring quantitative fluorescence and phase (or equivalently wavefront) images with a single camera sensor, avoiding any delay between both images, or registration of images acquired separately. The method is based on the use of a 2-dimensional diffraction grating (aka cross-grating) positioned at a millimeter distance from a 2-color camera. Fluorescence and wavefront images are extracted from the two color channels of the camera, and retrieved by image demodulation. The applicability of the method is illustrated on various samples, namely fluorescent micro-beads, bacteria and mammalian cells.
Non-invasive assessment and visualization of Phytophthora cactorum infection in strawberry crowns using quantitative magnetic resonance imaging
Authors: Tuomainen Teemu Valtteri , Toljamo Anna , Kokko Harri , Nissi Mikko Johannes 
Published: January 2024

AbstractPhytophthora cactorum is an oomycete species that causes enormous losses on horticultural crops, including strawberries. The purpose of this work was to investigate the alterations caused by P. cactorum inoculation in hydroponically grown strawberry plantlets (Fragaria × ananassa Duch.) using quantitative magnetic resonance imaging (qMRI). It was observed that with MRI, spatial and temporal progression of the infection could be observed in the crown using quantitative MR parameters, namely relaxation time maps. Relaxation times are numeric subject-specific properties that describe the MR signal behavior in an examined anatomical region. Elevated $$T_{2}$$

T
2

relaxation time values were observed inside the infected plant crowns with respect to the healthy references. The $$T_{2}$$

T
2

and $$T_{2}^{*}$$

T

2

∗

values of healthy plants were small in the crown region and further diminished during the development of the plant. Furthermore, elevated $$T_{1}$$

T
1

relaxation time values were seen in regions where P. cactorum progression was observed in corresponding plant dissection photographs. Quantitative susceptibility maps (QSM) were calculated to estimate the local magnetic field inhomogeneities. The QSM suggests magnetic susceptibility differences near the center of the pith. This study provides novel non-invasive information on the structure and development of strawberry plants and the effects caused by the P. cactorum infection.
Domain-wall dark pulse generation with SMF-GIMF-SMF structure as artificial saturable absorber
Authors: Chen Yu , Cheak Tiu Zian , Jin Tan Sin , Vinitha G. , Dimyati Kaharudin , Harun Sulaiman Wadi 
Published: January 2024

AbstractWe experimentally demonstrated the generation of domain-wall dark pulse in an Erbium-doped fiber laser using the combination of a 10 cm graded index multimode fiber sandwiched by single mode fibers as artificial saturable absorber. The interaction of phase difference in grade index multimode fiber allowed the stable dual-wavelength oscillation in the cavity. The dual-wavelength centered at 1567.2 nm and 1569.4 nm produces the topological defect in temporal domain and achieved a dark pulse formation with repetition rate of 21.5 MHz. The highest average pulse energy is calculated as 769.6 pJ with pulse width of 5 ns. Throughout the operating pump power range, the average pulse energy and output power increase linearly, with R2 of 0.9999 and achieved the laser efficiency of 9.33%. From the measurement in frequency domain, the signal-to-noise ratio is measured as 49 dB. As compared to reported DW dark pulse works, the proposed structure only required a short length of multimode fiber, which allowed the domain-wall dark pulse to achieve higher pulse repetition rate. The venture of domain wall dark pulse is potentially to pave the foundation toward sustainable industrial growth.
Tonsillar cytotoxic CD4 T cells are involved in the control of EBV primary infection in children
Authors: Amarillo María Eugenia , Moyano Agustina , Ferressini Gerpe Natalia , De Matteo Elena , Preciado Maria Victoria , Chabay Paola 
Published: January 2024

AbstractCD4 T cells play a key role in Epstein Barr virus (EBV) infection, by modulating latent antigen expression, and exhibiting cytotoxic and regulatory properties. Our aim was to evaluate the presence of Granzyme B (GZMB) and Foxp3 CD4 T cells at different EBV infection status and latency profiles. We examined CD4, GZMB, Foxp3, IL10, TGF-β, CD4-GZMB and CD4-Foxp3 expression at the tonsils of pediatric patients with different infective status and EBV latency profiles. CD4+, GZMB+, Foxp3+, CD4-GZMB+ and CD4-Foxp3+ cell counts were higher at the interfollicular region. Higher expression of CD4-GZMB was found in primary infected patients compared to healthy carriers. In patients that expressed latency III antigens, we demonstrated lower CD4+, CD4-GZMB+, CD4-Foxp3+ expression; a negative correlation between the immunoregulatory cytokine IL-10+ and GZMB+ as well as a positive correlation of IL-10+ and CD4+. In patients expressing the lytic protein BMRF1, a positive correlation of TGF-β+ with CD4-GZMB+ and CD4-Foxp3+ was observed. Our findings indicate that CD4-GZMB+ cells are involved in the restriction of primary EBV infection in pediatric patients, which could partially explain the lack of symptoms, whereas both CD4-GZMB+ and CD4-Foxp3+ cells could be involved in the modulation of latency.
Fabrication of high aspect ratio, non-line-of-sight vias in silicon carbide by a two-photon absorption method
Authors: Payne Jared E. , Nyholm Peter , Beazer Ryan , Eddy Joseph , Stevenson Hunter , Ferguson Brad , Schultz Stephen , Nielson Gregory N. 
Published: January 2024

AbstractThe future of Moore’s Law for high-performance integrated circuits (ICs) is going to be driven more by advanced packaging and three-dimensional (3D) integration than by simply decreasing transistor size. 3D ICs offer low-power consumption, high-performance and a smaller footprint compared to conventional 2D ICs. The key enabling technology to 3D integration is the interposer that provides interconnects to route signals between the chiplets that comprise the IC. However, the fabrication of high-aspect ratio through wafer vias (TWVs), that provide electrical and mechanical connection between chiplets on the top and bottom of the interposer, is one of the important challenges that limit interposer performance. Current fabrication technologies are limited by tapering effects and the need for direct line of sight to the fabrication surface. These limit the possible aspect ratios of vias and require large, complicated surface traces to connect the vias to the chiplets. Here, we demonstrate the fabrication of high-aspect ratio, non-line-of-sight TWVs in silicon carbide (SiC). SiC provides better mechanical, chemical, and thermal performance than silicon (Si). The technique uses an electro-chemical etch process that utilizes two-photon absorption to create any arbitrary 3D structure in SiC allowing for direct, subsurface routing between chiplets.