bluuebunny/crossref_metadata_2025_split huggingface dataset:
Possible stellar asterisms carved on a protohistoric stone
Authors: Molaro Paolo , Bernardini Federico 
Published: November 2023

AbstractChisel marks on a stone uncovered in Rupinpiccolo protohistoric hill fort from north‐eastern Italy were suggested to be a representation of the night sky (Bernardini et al. 2022 Documenta Praehistorica XLIX). The patterns of the 29 marks are analyzed here to establish if they reproduce popular stellar asterisms. Nine marks are found to match the Tail of Scorpius and five the Orion's Belt, together with Rigel and Betelgeuse. Nine marks are found in the approximate position of the Pleiades showing some match with the cluster members. On the back side, 5 marks possibly reproduce Cassiopeia. One mark slightly North of Orion cannot be identified. The 28 marks show a Pearson correlation coefficient (28) with stellar positions higher than 0.99 with a probability of a wrong correlation lower than 0.001. Departures are about one degree, or about 7 mm, as the mean diameter of the marks, which suggests a manufacturing limitation in the charting. The fort dates 1800–400 BCE when Scorpius and Orion showed about the same orientation at the heliacal rising. The unidentified mark challenges the whole picture. We suggest it could have been the progenitor of a failed supernova, thus offering also the possibility of a verification.
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation
Authors: Yan Ge , Lu Xun , Sun Ruiqiang , Zhou Weichang , Zhou Hang 
Published: November 2023

AbstractMammalian cells remain the mainstay of biological production host. In industry, cultivating and harvest strategies are sorted in batch mode (e.g., batch, fed‐batch, concentrated fed‐batch and intensified fed‐batch) and continuous mode (e.g., perfusion). To retrieve greater productivity and better product quality, especially for the sensitive products prone to fragmentation, culture modes with various modifications are innovated (e.g., intensified perfusion culture [IPC]). In our study, we demonstrated that the fragmentation of Fc‐fusion product (Molecule A) is time‐dependent in traditional fed‐batch (TFB) culture. The fragmentation proportion increased from 3.8% to 12.4% for Clone A, 0.8% to 1.7% for Clone B and 0.9% to 2.0% for Clone C from Day 10 to Day 14. By applying a novel bioprocess, IPC, which allows continuous feeding of the fresh medium and constant removal of the spent medium without bleeding cells to maintain a defined constant viable cell density, the fragmentation was reduced to 0.3% while the productivity was increased from 2.96 g/L to 15.51 g/L for Clone A. To validate whether the fragmentation reduction is product‐sensitive, plasmids carrying the DNA sequences of two other Fc‐fusion molecules (Molecule B and Molecule C) were transfected into the host. The results showed consistent fragmentation reducing effect by using IPC. Furthermore, the cultivation scale was expanded to 50 L and 1000 L. A minimum fragmentation level below 0.1% was observed for Molecule C. Our study revealed the capability of IPC in reducing Fc‐fusion protein fragmentation and the reproducibility when scaling up while maintaining high productivity.
The role of illness‐related cognition in the relationships between resilience and depression/anxiety in nasopharyngeal cancer patients
Authors: Wang Shenghao , Deng Yang , Zhang Yuan , Guo Vivian Yawei , Zhang Bo , Cheng Xi , Xin Meiqi , Hao Yuantao , Hou Fengsu , Li Jinghua 
Published: November 2023

AbstractObjectiveResilience has been reported as an important predictor of better mental health and prognoses in cancer patients, while its mechanisms were not clearly elucidated. In this study, we surveyed a large sample of nasopharyngeal carcinoma patients to investigate the mediating role of illness‐related cognition (illness perception, stigma and meaning in life) on the associations between resilience and symptoms of anxiety and depression.MethodsThis cross‐sectional study involved 773 participants diagnosed with nasopharyngeal carcinoma. Participants completed a self‐reported structured questionnaire to assess their illness perception, stigma and meaning in life, resilience and symptoms of anxiety and depression. Structural equation models (SEM) were employed to explore the relationship between resilience and symptoms of anxiety and depression in the entire sample, as well as in two subgroups: Subgroup I (0–1 year since diagnosis), and Subgroup II (over 1 year since diagnosis).ResultsIn the entire sample, after adjusting for potential confounders, illness perception, stigma and meaning in life were found to mediate the protective effect of resilience on symptoms of depression (mediating effect proportion: 65.25%) and anxiety (mediating effect proportion: 67.63%). In Subgroup I, direct effects were dominant in the associations between resilience and symptoms of anxiety (mediating effect proportion: 37.95%) and depression (mediating effect proportion: 29.13%). However, in Subgroup II, the associations between resilience and symptoms of anxiety (mediating effect proportion: 98.92%) and depression (mediating effect proportion: 81.04%) were completely mediated.ConclusionsOur study suggests that direct and indirect effects of resilience on depression and anxiety dominate in early periods (0–1 year) and long‐term periods (over 1 year) following the cancer diagnosis, respectively. The findings indicate that comprehensive intervention considering both the direct effect of resilience in early stages (e.g., health education prescription and social support groups) and the indirect effects of illness cognition in long‐term periods (e.g., cognitive behavioral therapies) are likely to yield the most favorable outcomes for cancer patients.
The effects of size referents in user‐generated photos on online review helpfulness
Authors: Dang Anh , Nichols Bridget Satinover 
Published: November 2023

AbstractProduct size misperceptions are a common problem for consumers when shopping online. To help their peers estimate a product's size, customers often include a size referent next to the focal product when adding photos to their online reviews. The purpose of this article is to investigate the effects of user‐provided review photos (with and without a size referent) on online review helpfulness. From two online experiments and one eye‐tracking lab experiment, this article finds that photo‐enhanced reviews that include a size referent object can improve review helpfulness. In study 1, eye‐tracking data shows that consumers pay greater attention to the photo and less to the text when the size referent is included. Study 2 shows that size referents are more helpful when consumers have limited experience with the product and the size referent is familiar. The role of perceived reviewer effort is investigated as a mediator to explain this result. Study 3 shows that the effects of a familiar size referent are robust when the seller provides detailed size information but vary depending on review valence.
Phytochemical Analysis and Evaluation of Genotoxic, Antigenotoxic Effects in Bacterial Models, and Hypoglycemic Activity of Epilobium angustifolium L. with Bioguided Isolation of Active Compounds
Authors: Yuca Hafize , Karadayı Mehmet , Karakaya Songül , Civaş Ayşe , Tekman Enes , Guvenalp Zuhal 
Published: November 2023

AbstractThis study examined the effects of methanol extract and its sub‐extracts from Epilobium angustifolium on α‐glucosidase and α‐amylase activity. Secondary metabolites and amino acids were quantified using LC–MS/MS. Dichloromethane sub‐extract displayed the highest activity and was chosen for further investigation. Despite the widespread use of E. angustifolium, genotoxicity studies were conducted to assess its safety. Dichloromethane significantly inhibited α‐glucosidase (IC50=17.340 μg/mL), making it approximately 293 times more effective than acarbose. Six known compounds, including gallic acid (1), a mixture of quercetin‐3‐O‐α‐galactoside (2a) and quercetin‐3‐O‐α‐glucoside (2b), quercetin‐3‐O‐α‐glucuronic acid (3), quercetin‐3‐O‐α‐rhamnoside (4), and kaempferol‐3‐O‐α‐rhamnoside (5) were identified. Quercetin‐3‐O‐α‐rhamnoside exhibited the highest inhibition of α‐glucosidase (IC50=1735±85 μM), making it 3.70 times more effective than acarbose. Dichloromethane also showed significant antigenotoxic activity against mutagenesis induced by NaN3, 9‐AA, 4‐NPD, and MNNG. Gallic acid was found in the highest abundance (13253.6931 ng/mL) in the methanolic extract. Furthermore, L‐Aspartic acid was the most concentrated amino acid (363.5620 nmol/mL) in the methanolic extract.
Mechanical circulatory support versus vasopressors alone in patients with acute myocardial infarction and cardiogenic shock undergoing percutaneous coronary intervention
Authors: Javaid Awad I. , Michalek Joel E. , Gruslova Aleksandra B. , Hoskins Serene A. , Ahsan Chowdhury H. , Feldman Marc D. 
Published: November 2023

AbstractBackgroundPrevious studies have compared Impella use to intra‐aortic balloon pump (IABP) use in patients with acute myocardial infarction and cardiogenic shock (AMI‐CS) undergoing percutaneous coronary intervention (PCI). Our objective was to compare clinical outcomes in patients with AMI‐CS undergoing PCI who received Impella (percutaneous left ventricular assist device) without vasopressors, IABP without vasopressors, and vasopressors without mechanical circulatory support (MCS).MethodsWe queried the National Inpatient Sample (NIS) using ICD‐10 codes (2015–2018) to identify patients with AMI‐CS undergoing PCI. We created three propensity‐matched cohorts to examine clinical outcomes in patients receiving Impella versus IABP, Impella versus vasopressors without MCS, and IABP versus vasopressors without MCS.ResultsAmong 17,762 patients, Impella use was associated with significantly higher in‐hospital major bleeding (31.4% vs. 13.6%; p < 0.001) and hospital charges (p < 0.001) compared to IABP use, with no benefit in mortality (34.1% vs. 26.9%; p = 0.06). Impella use was associated with significantly higher mortality (42.3% vs. 35.7%; p = 0.02), major bleeding (33.9% vs. 22.7%; p = 0.001), and hospital charges (p < 0.001), when compared to the use of vasopressors without MCS. There were no significant differences in clinical outcomes between IABP use and the use of vasopressor without MCS.ConclusionsIn this analysis of retrospective data of patients with AMI‐CS undergoing PCI, Impella use was associated with higher mortality, major bleeding, and in‐hospital charges when compared to vasopressor therapy without MCS. When compared to IABP use, Impella was associated with no mortality benefit, along with higher major bleeding events and in‐hospital charges. A vasopressor‐only strategy suggested no difference in clinical outcomes when compared to IABP. This study uses the NIS for the first time to highlight outcomes in AMI‐CS patients undergoing PCI when treated with vasopressor support without MCS, compared to Impella and IABP use.
Glucose Oxidase Patterned Meta‐Chemical Surface for Sensing Glucose Using Dip‐Pen Nanolithography
Authors: Saban Dganit , Shamir Dror , Zohar Moshe , Burg Ariela 
Published: November 2023

AbstractNumerous studies have been done to advance the development of efficient, simple, and cheap sensors, especially for glucose, which are important in the pharmaceutical and food industries. The most common sensors and biosensors used today are based on electrochemistry. The novelty of this study is the use of dip‐pen nanolithography (DPN) in order to pattern a mixture of glucose oxidase with PMMA as nanoclusters and develop meta‐chemical surface (MCS), which leads to highly efficient working electrodes with increased detection sensitivity compared to the today‐known glucose sensors (
M was detected in the current study). Our novel and important results support the feasibility of using DPN to develop sensors on various electrode surfaces patterned with different active species, especially in systems requiring target substance detection over large concentration ranges.
A New Series of Sandwich‐Type 5,5′‐Biterphenylenes: Synthetic Challenge, Structural Uniqueness and Photodynamics
Authors: Pan Ming‐Lun , Hsu Chao‐Hsien , Lin Yan‐Ding , Chen Wei‐Sen , Chen Bo‐Han , Lu Chih‐Hsuan , Yang Shang‐Da , Cheng Mu‐Jeng , Chou Pi‐Tai , Wu Yao‐Ting 
Published: November 2023

AbstractA new series of biaryls, bi‐linear‐terphenylenes (BLTPs), were prepared using the tert‐butyllithium‐mediated cyclization as the key synthetic step. The three‐dimensional structures of the studied compounds were verified using X‐ray crystallography and DFT calculations. Tetraaryl(ethynyl)‐substituted BLTPs are highly crowded molecules, and the internal rotation around the central C−C bond is restricted due to a high barrier (>50 kcal/mol). These structures contain several aryl/terphenylenyl/aryl sandwiches, where the through‐space π‐π (TSPP) interactions are strongly reflected in the shielding of 1H NMR chemical shifts, reduction of oxidation potentials, increasing aromaticity of the central six‐membered ring and decreasing antiaromaticity of the four‐membered rings in a terphenylenyl moiety based on NICS(0) and iso‐chemical shielding surfaces. Despite the restricted C−C bond associated intramolecular TSPP interactions for BLTPs in the ground state, to our surprise, the electronic coupling between two linear terphenylenes (LTPs) in BLTPs in the excited state is weak, so that the excited‐state behavior is dominated by the corresponding monomeric LTPs. In other words, all BLTPs undergo ultrafast relaxation dynamics via strong exciton‐vibration coupling, acting as a blue‐light absorber with essentially no emission.
Structurally Diverse Pyrene‐decorated Planar Chiral [2,2]Paracyclophanes with Tunable Circularly Polarized Luminescence between Monomer and Excimer
Authors: Lian Zhe , Liu Lin , He Jing , Fan Shimin , Guo Shengzhu , Li Xiaonan , Liu Guoqin , Fan Yanqing , Chen Xuebo , Li Meng , Chen Chuanfeng , Jiang Hua 
Published: November 2023

AbstractWe reported the synthesis of a series of structurally diverse CPL‐active molecules, in which pyrene units were installed to chiral pm/po‐[2,2]PCP scaffolds either with or without a triple bond spacer for pm/po‐PCP‐P1 and pm/po‐PCP‐P2, respectively. The X‐ray crystallographic analyses revealed that these pyrene‐based [2,2]PCP derivatives exhibited diverse structures and crystal packings in the solid phases. The pyrene‐based [2,2]PCP derivatives exhibit various (chir)optical properties in organic solutions, depending on their respective structures. In a mixture of dioxane and water, pm/po‐PCP‐P1 emit green excimer fluorescence, whereas pm/po‐PCP‐P2 emit blue one. The chiroptical investigation demonstrated that Rp‐pm‐PCP‐P1 and Rp‐pm‐PCP‐P2 exhibited completely opposite CD and CPL signals even they possess the same chiral Rp‐[2,2]PCP core. The same argument also holds for other chiral pyrene‐based [2,2]PCP derivatives. The theoretical calculation revealed that these unusual phenomena were attributed to different orientation between transition electric dipole moments and the magnetic dipole moments originating from the presence or absence of a triple bond spacer. These pyrene‐based [2,2]PCP derivatives display various colours and fluorescence emissions in the solid state and PMMA films, possibly due to the different packings as observed in the crystal structure. Moreover, these compounds also can interact with perylene diimide through π‐π interactions, leading to near‐white fluorescence.
Integration of single‐cell and bulk RNA‐sequencing to analyze the heterogeneity of hepatocellular carcinoma and establish a prognostic model
Authors: Mu Yaping , Zheng Ding , Peng Qinghua , Wang Xiaodong , Zhang Yurong , Yin Yue , Wang Encheng , Ye Fei , Wang Jing 
Published: November 2023

AbstractBackgroundThe highly heterogeneous nature of hepatocellular carcinoma (HCC) results in different responses and prognoses to the same treatment in patients with similar clinical stages.AimsThus, it is imperative to investigate the association between HCC tumor heterogeneity and treatment response and prognosis.Methods and ResultsAt first, we downloaded scRNA‐seq, bulk RNA‐seq, and clinical data from TCGA and GEO databases. We conducted quality control, normalization using SCTransform, dimensionality reduction using PCA, batch effect removal using Harmony, dimensionality reduction using UMAP, and cell annotation‐based marker genes on the scRNA‐seq data. We recognized tumor cells, identified tumor‐related genes (TRGs), and performed cell communication analysis. Next, we developed a prognostic model using univariable Cox, LASSO, and multivariate Cox analyses. The signature was evaluated using survival analysis, ROC curves, C‐index, and nomogram. Last, we studied the predictability of the signature in terms of prognosis and immunotherapeutic response for HCC, assessed a variety of drugs for clinical treatment, and used the qRT‐PCR analysis to validate the mRNA expression levels of prognostic TRGs.ConclusionTo conclude, this study expounded upon the influence of tumor cell heterogeneity on the prediction of treatment outcomes and prognosis in HCC. This, in turn, enhances the predictive ability of the TNM staging system and furnishes novel perspectives on the prognostic assessment and therapy of HCC.
Capacity to consent to psychotherapy: Reliability of the newly adapted German version of the MacArthur Competence Assessment Tool for Treatment for psychotherapy
Authors: Ladwig Sönke , Pauls Franz , Gerke Leonie , Trachsel Manuel , Nestoriuc Yvonne 
Published: November 2023

AbstractBackgroundPatients' capacity to consent to treatment (CCT) is a prerequisite for ethically sound informed consent in psychotherapy. The MacArthur Competence Assessment Tool for Treatment (MacCAT‐T) is a reliable instrument for assessing CCT. A German version was adapted to the psychotherapeutical context (MacCAT‐PT) to investigate its reliability and possible influences of age, education and prior experience with psychotherapy on CCT in a mixed clinical sample.MethodsN = 108 patients with indication for psychotherapy were recruited. The MacCAT‐PT was administered by trained psychologists, took 20 min on average and was rated by the administering psychologist and an independent rater. Reliability statistics were investigated and regression analyses were conducted on MacCAT‐PT scores and sociodemographic variables.ResultsSufficient to moderate inter‐rater reliability (ICC = 0.80) and internal consistency (α = 0.80) were found for the total sum score of the MacCAT‐PT and its scales, Understanding (ICC = 0.79, α = 0.77), Reasoning (ICC = 0.57, α = 0.65) and Making a Choice (ICC = 0.57). Appreciation featured an unacceptable inter‐rater reliability (ICC = −0.01). Regression analyses indicated no significant effects.ConclusionThese findings suggest that the MacCAT‐PT is a reliable tool for assessing patients' overall CCT in psychotherapy. Psychometric properties of three scales were of good quality, while Appreciation needs to be reanalysed in patient samples with lower motivation for psychotherapy or limited CCT. The CCT may be suggested to be independent of age, education and prior experience. Future research should provide analyses focusing on structural and clinical validity in multiple clinical samples.
Canine Models of Inflammatory Skin Diseases and Their Application in Pharmacological Research
Authors: Gil Natalia , Santoro Domenico 
Published: November 2023

AbstractThe purpose of this article is to provide an overview of existing pharmacological models of canine dermatitis. Canine models of dermatitis have contributed significantly to our current understanding of the pathology of dermatitis and to the development of corresponding pharmacological interventions. Specifically, canine atopic dermatitis (AD) is reviewed here, as it is one of the most common inflammatory skin diseases in dogs. Canine AD also shares clinicopathological features with human AD, making the dog a natural and optimal model for human disease. Thus, pharmacological models of canine AD may be uniquely applicable to human pharmacological research. In this article, particular attention is dedicated to relevant in vivo, in vitro, and ex vivo models of canine AD, skin barrier defect models, pruritus models, and skin immunology models. Additionally, models of superficial pyoderma and food allergy are also discussed. With understanding of findings from canine models, researchers can select the most salient features for future pharmacological drug development. © 2023 Wiley Periodicals LLC.
DFT Study of N‐modified Co3Mo3C Electrocatalyst with Separated Active Sites for Enhanced Ammonia Oxidation
Authors: Yang Xue Jing , Yang Chun Cheng , Jiang Qing 
Published: November 2023

AbstractSince the facile oxidation of ammonia is one key for its utilization as a zero‐carbon fuel in a direct ammonia fuel cell, developing the ammonia oxidation reaction (AOR) catalysts with cost‐effective and higher activity is urgently required. However, the catalytic activity of AOR is limited by the scaling relationship of the intermediate adsorption. Based on the density functional theory, the N‐modified Co3Mo3C with separated active sites of NH3 dehydrogenation and N−N coupling has been designed and investigated, which is a promising strategy to circumvent the scaling relationship, achieving improved AOR catalytic performance with a lower theoretical overpotential of 0.59 V under fast reaction kinetics condition. The calculation results show that the hollow site (Co−Mo−Mo and Co−Co−Mo) and Co site in N‐modified Co3Mo3C play essential roles in NH3 dehydrogenation and N−N coupling, respectively. This work not only benefits for understanding the mechanism of AOR, but also provides a fundamental guidance for rational design of AOR catalysts.
Fine needle aspiration as a diagnostic modality for Warthin tumors identified as fluorodeoxyglucose positron emission tomography/computed tomography‐positive
Authors: Kowalewski Adam , Tissot Hubert , Jehanno Nina , Bakuła Zalewska Elwira , Choussy Olivier , Lesnik Maria , Badois Nathalie , Rougier Guillaume , Klijanienko Jerzy 
Published: November 2023

AbstractLimited evidence exists regarding the 2‐deoxy‐2‐[fluorine‐18]‐fluoro‐D‐glucose (FDG) avidity of Warthin tumors, the second most common benign parotid gland tumor. This study aims to clarify this aspect by analyzing patients who underwent FDG positron emission tomography/computed tomography (PET/CT) and quantifying tumor standardized uptake values (SUV). Medical records of 29 patients with fine needle aspiration (FNA)‐confirmed Warthin tumors who underwent FDG‐PET/CT near the diagnosis of Warthin tumor were reviewed. Key parameters included cancer history, cytologic diagnosis of Warthin tumor, maximum SUV on FDG PET/CT, and tumor localization. Among the cohort, 18 males and 11 females (average age: 67.9 years) were included. Most patients had malignant neoplasms (lung, head and neck, breast, others). One patient had synchronous liver cancer. Three individuals had bilateral Warthin tumors, and three had bifocal tumors, resulting in 35 tumors for analysis. Tumors were located in the parotid gland (28) and vicinity (7). SUVmax for the Warthin tumors ranged from 3.6 to 26.8, with an average SUVmax of 10.1. Warthin tumors exhibit significant and variable FDG accumulation, exceeding expectations and mimicking high‐grade malignancies. Awareness of this phenomenon is crucial for accurate staging and timely management. In cases of positive FDG PET/CT uptake in periparotid, perimandibular, and upper jugular areas, FNA is recommended to avoid misinterpretation or delays in management.