bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrastructural Characteristics and Synaptic Connectivity of Photoreceptors in the Simplex Retina of Little Skate (Leucoraja erinacea)
Authors: Magaña-Hernández Laura , Wagh Abhiniti S. , Fathi Jessamyn G. , Robles Julio E. , Rubio Beatriz , Yusuf Yaqoub , Rose Erin E. , Brown Daniel E. , Perry Priscilla E. , Hamada Elizabeth , Anastassov Ivan A. 
Published: October 2023

AbstractThe retinas of the vast majority of vertebrate species are termed “duplex,” that is, they contain both rod and cone photoreceptor neurons in different ratios. The retina of little skate (Leucoraja erinacea) is a rarity among vertebrates because it contains only a single photoreceptor cell type and is thus “simplex.” This unique retina provides us with an important comparative model and an exciting opportunity to study retinal circuitry within the context of a visual system with a single photoreceptor cell type. What is perhaps even more intriguing is the fact that theLeucorajaretina is able use that single photoreceptor cell type to function under both scotopic and photopic ranges of illumination. Although some ultrastructural characteristics of skate photoreceptors have been examined previously, leading to a general description of them as “rods” largely based on outer segment (OS) morphology and rhodopsin expression, a detailed study of the fine anatomy of the entire cell and its synaptic connectivity is still lacking. To address this gap in knowledge, we performed serial block-face electron microscopy imaging and examined the structure of skate photoreceptors and their postsynaptic partners. We find that skate photoreceptors exhibit unusual ultrastructural characteristics that are either common to rods or cones in other vertebrates (e.g., outer segment architecture, synaptic ribbon number, terminal extensions), or are somewhere in between those of a typical vertebrate rod or cone (e.g., number of invaginating contacts, clustering of multiple ribbons over a single synaptic invagination). We suggest that some of the ultrastructural characteristics we observe may play a role in the ability of the skate retina to function across scotopic and photopic ranges of illumination. Our findings have the potential to reveal as yet undescribed principles of vertebrate retinal design.
Tetherin antagonism by SARS‐CoV‐2 ORF3a and spike protein enhances virus release
Authors: Stewart Hazel , Palmulli Roberta , Johansen Kristoffer H , McGovern Naomi , Shehata Ola M , Carnell George W , Jackson Hannah K , Lee Jin S , Brown Jonathan C , Burgoyne Thomas , Heeney Jonathan L , Okkenhaug Klaus , Firth Andrew E , Peden Andrew A , Edgar James R 
Published: October 2023

AbstractThe antiviral restriction factor, tetherin, blocks the release of several different families of enveloped viruses, including the Coronaviridae. Tetherin is an interferon‐induced protein that forms parallel homodimers between the host cell and viral particles, linking viruses to the surface of infected cells and inhibiting their release. We demonstrate that SARS‐CoV‐2 infection causes tetherin downregulation and that tetherin depletion from cells enhances SARS‐CoV‐2 viral titres. We investigate the potential viral proteins involved in abrogating tetherin function and find that SARS‐CoV‐2 ORF3a reduces tetherin localisation within biosynthetic organelles where Coronaviruses bud, and increases tetherin localisation to late endocytic organelles via reduced retrograde recycling. We also find that expression of Spike protein causes a reduction in cellular tetherin levels. Our results confirm that tetherin acts as a host restriction factor for SARS‐CoV‐2 and highlight the multiple distinct mechanisms by which SARS‐CoV‐2 subverts tetherin function.
Downregulation of stromal syntenin sustains AML development
Authors: Leblanc Raphael , Ghossoub Rania , Goubard Armelle , Castellano Rémy , Fares Joanna , Camoin Luc , Audebert Stephane , Balzano Marielle , Bou‐Tayeh Berna , Fauriat Cyril , Vey Norbert , Garciaz Sylvain , Borg Jean‐Paul , Collette Yves , Aurrand‐Lions Michel , David Guido , Zimmermann Pascale 
Published: October 2023

AbstractThe crosstalk between cancer and stromal cells plays a critical role in tumor progression. Syntenin is a small scaffold protein involved in the regulation of intercellular communication that is emerging as a target for cancer therapy. Here, we show that certain aggressive forms of acute myeloid leukemia (AML) reduce the expression of syntenin in bone marrow stromal cells (BMSC). Stromal syntenin deficiency, in turn, generates a pro‐tumoral microenvironment. From serial transplantations in mice and co‐culture experiments, we conclude that syntenin‐deficient BMSC stimulate AML aggressiveness by promoting AML cell survival and protein synthesis. This pro‐tumoral activity is supported by increased expression of endoglin, a classical marker of BMSC, which in trans stimulates AML translational activity. In short, our study reveals a vicious signaling loop potentially at the heart of AML–stroma crosstalk and unsuspected tumor‐suppressive effects of syntenin that need to be considered during systemic targeting of syntenin in cancer therapy.
The transcription factor HIF2α partakes in the differentiation block of acute myeloid leukemia
Authors: Magliulo Daniela , Simoni Matilde , Caserta Carolina , Fracassi Cristina , Belluschi Serena , Giannetti Kety , Pini Raffaella , Zapparoli Ettore , Beretta Stefano , Uggè Martina , Draghi Eleonora , Rossari Federico , Coltella Nadia , Tresoldi Cristina , Morelli Marco J , Di Micco Raffaella , Gentner Bernhard , Vago Luca , Bernardi Rosa 
Published: October 2023

AbstractOne of the defining features of acute myeloid leukemia (AML) is an arrest of myeloid differentiation whose molecular determinants are still poorly defined. Pharmacological removal of the differentiation block contributes to the cure of acute promyelocytic leukemia (APL) in the absence of cytotoxic chemotherapy, but this approach has not yet been translated to non‐APL AMLs. Here, by investigating the function of hypoxia‐inducible transcription factors HIF1α and HIF2α, we found that both genes exert oncogenic functions in AML and that HIF2α is a novel regulator of the AML differentiation block. Mechanistically, we found that HIF2α promotes the expression of transcriptional repressors that have been implicated in suppressing AML myeloid differentiation programs. Importantly, we positioned HIF2α under direct transcriptional control by the prodifferentiation agent all‐trans retinoic acid (ATRA) and demonstrated that HIF2α blockade cooperates with ATRA to trigger AML cell differentiation. In conclusion, we propose that HIF2α inhibition may open new therapeutic avenues for AML treatment by licensing blasts maturation and leukemia debulking.
Una mirada a la labor docente en asignaturas E-Learning
Authors: Sandoval Valero Erika María , Ruiz Cañadulce Edith Marcela , Pardo Santamaría Diego Fernando , Castro Molano Ivonne Liliana 
Published: October 2023

Este artículo presenta aspectos relevantes, estrategias y estadísticas relacionadas con el proceso de seguimiento para ser un buen tutor virtual en la orientación de una asignatura e_learning orientada en una institución de educación superior en Colombia. El estudio fue realizado en los dos semestres académicos ofrecidos en el año 2022.
El primer apartado del artículo muestra un panorama sobre lo que significa el seguimiento en otras instituciones educativas y el diseño metodológico de este ejercicio investigativo, el segundo apartado describe el proceso de seguimiento realizado en una institución de educación superior (IES) mostrando ítems de evaluación, posteriormente se muestran las estadísticas que los evidencian para los dos semestres del año 2022 y por último se encuentran las conclusiones.
Rainbow without the Sun: Textual Refraction in Soviet-era Translations
Authors: Subačius Paulius V. 
Published: October 2023

Light waves change direction—refract—as they pass through different environments. Similarly, Western literary works, even after leaking through the barrier of the Soviet Index Librorum Prohibitorum, never reached the USSR reader in their authentic form. The most evident components of the unfavourable environment were the proxy of the Moscow Raduga and Progress publishing houses, ideologically committed literary criticism, the hunt for honorariums, the lack of reference books, and the poverty of printing technology. All these adverse factors together caused the chronic syndrome of texts that seemed authentic but in fact were not. So far, individual agents and effects of textual refraction have been researched, and interesting case studies have been carried out. However, there is a lack of both a systematic classification of the different agents of influence and an assessment of their long-term effects. The methodological study of refraction-affected editions tests the possibility of integrating the concept of textual condition with the assertions of the postcolonial theory regarding secondary cultural colonisation. The paper attempts to raise the hypothesis that the efforts of professional translators and editors to perform their work with the utmost craftsmanship had sometimes paradoxically reinforced the refraction of the overall meaning of major literary works. That resulted from the contrast between a relatively reliable translation and the implications generated by the bibliographic code and metatexts, as well as from the canonisation of translations that had become iconic in the Soviet-era.
Characterization of disinfectant susceptibility profiles among clinical isolates of Acinetobacter baumannii in Ardabil, Iran
Authors: Namaki Kheljan Malek , Hassanzadeh Malihe , Srdari Jabedar Mehran , Mohammadi Gollou Ali , Ashouri Parastoo , Teimourpour Roghayeh , Arzanlou Mohsen 
Published: October 2023

AbstractAntimicrobial disinfectants have been extensively used to control hospital-acquired infections worldwide. Prolonged exposure to bacteria could promote resistance to antimicrobial disinfectants. This study evaluated the antimicrobial activity of four commonly used disinfectants; triclosan, chlorhexidine digluconate, benzalkonium chloride, and formaldehyde against Acinetobacter baumannii clinical isolates. This study also determined the prevalence and association of efflux pumps encoding genes qacE, qacED1, emrA, and aceI with tolerance to disinfectants. A total of 100 A. baumannii isolates were included in the current study. The antimicrobial disinfectants' minimum inhibitory concentration (MIC) was determined using an agar dilution method. Genes involved in resistance to disinfectants were investigated by PCR method. The benzalkonium chloride MICs ranged between 32 and 128 μg mL−1, chlorhexidine digluconate 8–64 μg mL−1, triclosan 1–32 μg mL−1, and formaldehyde 128 μg mL−1. Overall, the highest MIC90 value was identified for formaldehyde (128 μg mL−1), followed by benzalkonium chloride and chlorhexidine digluconate (64 μg mL−1, each one) and triclosan (4 μg mL−1). In the present study, the qacE, qacED1, emrA, and aceI genes were found in 91%, 55%, 100%, and 88% of isolates, respectively. The qacG gene was not identified in our A. baumannii isolates. The qacED1 gene was associated with higher MICs for all disinfectants tested (P < 0.05), while the qacE and aceI genes were associated with higher MICs for benzalkonium chloride and chlorhexidine. This study indicated that triclosan is the most effective disinfectant against A. baumannii isolates.
White spot lesions in clear aligner therapy as compared with multi-bracket system: A Systematic Review
Authors: Sharma Vipul K , Singh Deepak , Srivastava Roopal , Priyadarshani Pooja , Chaturvedi T P 
Published: October 2023

To evaluate incidence of white spot lesions in clear aligner treatment compared with fixed appliance therapy.Electronic databases (PubMed, Cochrane Collaboration Oral Health group database of clinical trials, Web of Science, Science Direct, Scopus) and manual search of gray literature were conducted up to September 2021. Both randomized and non-randomized controlled studies were considered for review purposes. Study selection, data extraction, quality assessment (RoB 2.0 and ROBINS-I) was performed according to the Cochrane Handbook for Systematic Reviews of Intervention. Due to the presence of high heterogeneity only qualitative analysis has been done.Out of 479 retrieved literature, 3 studies fulfilled the eligibility criteria and were included in the analysis. Studies were evaluated at low to high risk of bias. On qualitative analysis, a total of 160 new white spot lesions were developed in the clear aligner group and 362 new lesions were found in the fixed orthodontic group (P &#60;0.001). In addition, the surface area of white spot lesion in the clear aligner group was more than the fixed appliance group but the deepest lesion was still found in the fixed appliance group. Incidence and severity of white spot lesion was found more in the fixed appliance group as compared to the clear aligner group. However, more low risk of bias studies with proper methodological quality is required to reinforce the conclusive evidence.
Curbing cost overruns in infrastructure investment
Authors: Park Jung Eun 
Published: October 2023

Infrastructure projects around the world have long been notorious for exceeding their budgets. To address persistent cost overruns, the American Planning Association urged planners to adopt reference class forecasting alongside traditional methods but the practice has not caught on in the U.S. Conversely, the U.K. adopted Kahneman’s Nobel Prize-winning theory to challenge biases in human judgment and mandated reference class forecasting for major projects in 2003. Has reference class forecasting, originally developed to rectify honest mistakes, brought the promised success in the public sector wherein political pressure is significant? Through before-and-after and with-and-without comparisons of 107 major projects, this empirical study examines the practical relevance of reference class forecasting for infrastructure investments. A before-and-after comparison reveals that the average cost overrun declined from 38% to 5% following the introduction of reference class forecasting. A with-and-without comparison also demonstrates that the U.K. surpassed its targeted probability of completing projects within budget by 12% using reference class forecasting, whereas the U.S. underperformed by 17%. Thus, reference class forecasting has engendered notable improvements in estimation in the U.K. This empirical study demonstrates the benefits of supplementing or replacing the current forecasting method. The findings can be used to reduce substantial financial risks for the government as well as social and economic welfare losses for society.
Pengaruh Kualitas Pelayanan Islam terhadap Loyalitas Pelanggan dengan Mediasi Kesenangan Pelanggan pada Penginapan Syariah di Mojokerto
Authors: Kurnia Fachreza Eka , Hendratmi Achsania , Anam Saiful , Ivanda Muhammad Nur Miftakhul 
Published: October 2023

This study's objective is to investigate the relationship between the quality of sharia-compliant customer service and the degree of customer loyalty, with the satisfaction of customers serving as a moderating variable. The participants in this study were customers who had previously utilised the services provided by sharia inns in Mojokerto. The research was carried out in a number of sharia inns in Mojokerto. The data analysis technique uses Regression Path Analysis. The empirical research results prove that service quality influences customer loyalty and customer satisfaction, and customer satisfaction influences customer loyalty. The results of the study also found that customer satisfaction mediates the effect of service quality on customer loyalty. In an increasingly competitive business era, service quality and customer satisfaction play a very important role for a company's success. Good service quality and customer delight can help build long-term relationships with customers. When customers are satisfied with the services provided, they tend to remain loyal and not switch to competitors.
Keywords: Customer Loyalty, Customer Satisfaction, Islam Service Quality
Pengaruh Variabel Macroeconomic terhadap Firm Performance pada Sektor LQ45
Authors: Suprapto Yandi , Junita Junita 
Published: October 2023

Two years aftar the global recession due to the pandemic caused by COVID-19, fears of a global recession have emerged due to the return of inflation triggered by the increase in interest rate by central banks in several countries, including Indonesia. This research is conducted to examine macroeconomic indicators’ effect, namely gross domestic product, inflation, exchange rate, interest rate and unemployment rate on firm performance. This study used 28 companies listed in the LQ45 sector during 2017-2021 consecutively. Panel regression method is chosen as the method of this research as panel data is analyzed. The results of the study show that the variables such as gorss domestic product, inflation, interest rate, unemployment rate have a significant effect, while the exchange rate variable does not have a significant effect on firm performance
Keywords: Gross domestic product, Inflation, Exchange rate, Interest rate, Unemployment rate
Fasulye genotiplerinde çok değişkenli istatistiksel yöntemler ile tohum fiziksel özelliklerinin karakterizasyonu
Authors: KARAMAN Ruziye 
Published: October 2023

Amaç: Tohumların hasat, harman, işlenmesi, sınıflandırılması ve depolanmasında tohumun fiziksel özellikleri büyük önem taşımaktadır. Bu nedenle çalışmada farklı büyüklük ve renkteki fasulye genotiplerinin fiziksel özellikleri kullanılarak çok değişkenli istatistik yöntemleri ile karakterizasyonun yapılması amaçlanmıştır.
Materyal ve Yöntem: Çalışma Isparta Uygulamalı Bilimler Üniversitesi Ziraat Fakültesi Tarla Bitkileri bölümü laboratuvarlarında 2021 yılında kurulmuştur. Araştırmada fiziksel özellik olarak tohumların uzunluğu, genişliği, kalınlığı, en-boy oranı, parlaklık (L*) değeri, aritmetik ortalama çapı, geometrik ortalama çapı, basıklığı, küreselliği, tohum hacmi, yığın hacim ağırlığı, gerçek hacim ağırlığı, porozite ve yüzey alanı özellikleri incelenmiştir. Çalışmada elde edilen veriler doğrultusunda korelasyon, temel bileşen analizi ve kümeleme ısı haritaları belirlenmiştir.
Araştırma Bulguları: Çalışma sonucunda fiziksel özellikler yönünden genel olarak Bolu’dan temin edilen G10 genotipi ön plana çıkmıştır. Korelasyon analizi sonucu genel olarak tohum uzunluğu, genişliği ve kalınlığı, aritmetik ortalama çap, geometrik ortalama çap ve tohum hacmi özellikleri arasında pozitif ilişki olduğu belirlenmiştir. TBA sonucunda incelenen özellikler ile ilgili olarak birbirinden bağımsız 3 tane temel bileşen ekseni elde edilmiştir. Elde edilen eksenlerin toplam varyasyonun %91.7’sini oluşturmaktadır. Kümeleme analizine göre ise, genotipler dört gruba, incelenen özellikler ise üç gruba ayrılmıştır.
Sonuç: Yapılan bu çok değişkenli analizlerin sonucunda belirlenen özelliklerin fasulye genetik materyalleri üzerine ıslahçılara pratik anlamda fayda sağlayacağı düşünülmektedir.
Optimal scheduling of electricity-gas-heat-hydrogen integrated energy system considering carbon transaction cost
Authors: Qiao Jian , Yin Xiang-Gen , Lu Qinghui , Liu Zhichang , Wang Yikai , Zhu Lingjin , Yin Xin 
Published: October 2023

Aim: To improve energy efficiency, optimize the operation of equipment, and reduce the carbon emission of the integrated energy system (IES), an IES low-carbon operation strategy is proposed.
Methods: This paper comprehensively considers the influence of the stepped carbon trading scheme, the wind generation and load forecasting represented by fuzzy parameters, and the operation strategy of energy storage equipment with flexible space margin on the optimal scheduling of IES. Based on this, a low-carbon operation target with the minimum sum of power purchase cost, gas purchase cost, carbon emission cost, and abandoned wind cost is constructed.
Results: Five scenarios are compared and analyzed. The effects of different carbon emission penalty strategies and wind power output and load uncertainties on benefits are analyzed. The feasibility of the proposed scheme is verified by the simulation results of a numerical example.
Conclusion: The stepwise carbon trading mechanism can better guide the effect of carbon emission reduction compared with the traditional carbon trading pricing model.
KELAYAKAN EKOWISATA MANGROVE LUPPUNG BERBASIS POTENSI KEANEKARAGAMAN HAYATI
Authors: Sribianti Irma , Hikmah Hikmah , Muthmainnah Muthmainnah , Abdullah Andi Azis , Tahnur Muhammad , Melati Reski 
Published: October 2023

Penelitian ini bertujuan untuk mengetahui nilai kelayakan ekowisata Hutan Mangrove Luppung. Metode analisis data berpedoman pada Pedoman Analisis Wilayah Kerja Objek dan Daya Tarik Wisata Alam (ADO-ODTWA) Direktorat Jenderal Perlindungan Hutan dan Konservasi Alam Tahun 2003 dianalisis menggunakan skoring sesuai dengan kriteria yang terdapat dalam Pedoman Analisis Objek Daerah Operasi dan Daya Tarik Wisata Alam dari Direktorat Jenderal Perlindungan Hutan dan Alam Konservasi tahun 2003. Perhitungan untuk masing-masing kriteria ini menggunakan tabulasi dimana diperoleh angka dari hasil penilaian yang nilai bobotnya berpedoman pada Penilaian Wilayah Kerja Obyek dan Daya Tarik Wisata Alam Direktorat Jenderal Perlindungan Hutan dan Konservasi 2003. Metode Penilaian Kelayakan Ekowisata dengan nilai yang telah ditentukan untuk setiap kriteria. Nilai kelayakan ekowisata Hutan Mangrove sebesar 70,8% masuk dalam kategori layak untuk dikembangkan sebagai objek wisata
KAJIAN EKOWISATA BERBASIS KESESUAIAN KAWASAN PANTAI SIRIMATA NEGERI TITAWAE KABUPATEN MALUKU TENGAH
Authors: Musa Katanyane Musa Katanyane , Lelloltery Henderina , Seipala Billy.B. 
Published: October 2023

Penelitian ini bertujuan untuk mengetahuai kesesuaian kawasan pantai untuk pengembangan ekowisata di Pulau Nusalaut. Metode yang digunakan adalah metode survei, dengan pengambilan data primer dan sekunder melalui teknik observasi. Pengambilan data primer meliputi data lingkungan perairan, lingkungan fisik pantai, luas areal yang digunakan untuk kegiatan wisata pantai dan waktu yang digunakan oleh wisatawan di kawasan pantai. Analisis data menggunakan indeks kesesuaian pantai. Kawasan yang berpasir sirimata dengan panjang 1,8 km. Kawasan berpasir putih dengan lebar 15 - 20 meter memberikan ruang bagi berbagai kegiatan wisata, ditunjang oleh pemandangan alam laut yang indah, serta kecerahan perairan yang tinggi (90 %), kecepatan arus yang rendah (0,16 m/det) menyebabkan pantai ini dapat menjadi destinasi wisata yang menjanjikan. Hasil analisis indeks kesesuaian ekowisata pantai menunjukan bahwa kawasan pantai sirimata negeri Titawae “Sangat Sesuai” untuk kegiatan wisata dengan nilai IKW = 95, 23 %