bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can inactivation mutation in the thyroid stimulating hormone receptor gene and hyperthyroidism coexist?: A case report
Authors: Liu Yanfang , Li Jie , Gao Fei , Zhao Changjian , Yang Luyang , Liu Yunfeng 
Published: January 2024

Introduction:We found the G132R heterozygous mutation of thyroid stimulating hormone receptor (TSHR) gene in a patient with recurrent hypokalemia. Because the patient had a medical history of hyperthyroidism, the mutation was suspected to be related to hyperthyroidism at first. Subsequently, the expression and function studies in vitro were conducted.Methods:Wide-type TSHR and mutant TSHR (mutTSHR) were constructed in the phage vector and pEGFP-C1 vector. After transfection, the samples were collected for detection of mRNA level, protein expression, cell activity and cAMP content.Results:Compared with the wild-type TSHR, the mRNA level of the mutTSHR was not significantly different. But the protein expression, cell activity and cAMP content of the mutTSHR were significantly lower. So this indicated that the G132R mutation is a loss-of-function mutation.Conclusion:We identified the G132R monoallelic heterozygous mutation of TSHR gene in a patient with hyperthyroidism. Based on disease history of the patient, we speculated that the heterozygous mutation did not cause thyroid dysplasia or hypothyroidism for her. Our study enriched experiment content in vitro studies and clinical phenotype about the G132R mutation in TSHR gene.
Identification of a novel genomic variance of BRAF1 in papillary thyroid carcinoma: A case report
Authors: Wang Yuguo , Zhao Jian , Tan Zhihan , Du Jing , Zhang Linping , Xu Ying , Li Xiuying , Cai Yun , Wang Hui , Jiang Jianjun 
Published: January 2024

Rationale:
Papillary thyroid carcinoma (PTC), the predominant subtypes accounting for approximately 85% of thyroid carcinomas, has a rapidly increasing global incidence rate. Statistically, approximately 74.6% PTC patients had the genomic variants of BRAF, especially BRAFV600E mutation, which has been reported to stratify patients and guide clinic-therapies. However, some PTC patients may carry other nonclassical mutation patterns of BRAF, due to the complex of genomic instability. And the spectrum of BRAF mutation was not fully characterized. We reported a novel BRAF mutation pattern of PTC.

Patient concerns:
A 59-year-old woman was admitted to our hospital because of the slight enlargement of bilateral cervical lymph nodes in July 2023.

Diagnosis:
Ultrasonography revealed that the bilateral thyroid nodules of the patients both presented 1 hypoechoic nodule, which was graded as 3 of the elastic score, and the small calcification in the right lobe (Chinese-Thyroid Imaging Reporting and Data System 4c). Pathological diagnosis showed the interstitial collagen change and focal follicular epithelial papillary hyperplasia with atypical hyperplasia of the bilateral thyroid. Further puncture pathology showed that the patient had a malignant thyroid lesion with the phenotypes of papillary carcinoma and diagnosed with malignancy subsequently. Additionally, the patient harbored a novel insert on BRAF exon 15, a 6-base fragment AGACAG inserting between c.1798 and c.1799.

Interventions:
The patient was undergone on microwave ablation of thyroid carcinoma on July 28, 2023. After the surgery, the patient was treated on anti-infection, cold saline external application of bilateral thyroid swelling supportive treatment.

Outcomes:
No postoperative complications or recurrence and metastasis were found.

Lessons:
This is the first case of the novel nonclassical genomic variant of BRAF. Our study extends the spectrum of BRAF mutations. The patient had a favorable response to microwave ablation, indicating that in spite of the association between this mutation and high-grade malignant phenotype, this genomic variant of BRAF did not have a detrimental effect on the response of clinical treatment.
Successful treatment of 1 patient with chlorine-induced ARDS using awake self-prone positioning and nasal high-flow oxygen: A case report
Authors: Wang Fugui , Liu Fangfang , Lu Houqing 
Published: January 2024

Rationale:
Accidents involving chlorinated compounds in the context of cleaning are not uncommon. However, improving the treatment success rate for acute respiratory distress syndrome (ARDS) patients caused by chlorine gas presents significant challenges.

Patient concerns:
A 28-year-old female was admitted to the intensive care unit after accidental inhalation of chlorine gas resulting in ARDS.

Diagnoses:
The patient was diagnosed with ARDS attributed to chlorine gas exposure.

Interventions:
The intervention involved utilizing a combination of awake self-prone positioning (ASPP) and high-flow nasal oxygen therapy for treatment.

Outcomes:
After continuous ASPP and high-flow nasal oxygen therapy, the patient quickly recovered and was transferred out of the intensive care unit on the 6th day without any adverse events.

Lessons:
ASPP combined with high-flow nasal oxygen therapy can improve patients’ hypoxemia, prevent the need for intubation, avoid rapid deterioration of the condition, reduce treatment complexity, and lower mortality rate.
Implementation of Guidelines Limiting Postoperative Opioid Prescribing at a Children’s Hospital
Authors: Stephenson Krista J. , Krinock Derek J. , Vasquez Isabel L. , Shewmake Connor N. , Spray Beverly J. , Ketha Bavana , Wolf Lindsey L. , Dassinger Melvin S. 
Published: January 2024

Objectives
Variability in opioid-prescribing practices after common pediatric surgical procedures at our institution prompted the development of opioid-prescribing guidelines that provided suggested dose limitations for narcotics. The aims of this study were to improve opioid prescription practices through implementation of the developed guidelines and to assess compliance and identify barriers preventing guideline utilization.

Methods
We conducted a single-center cohort study of all children who underwent the most common outpatient general surgery procedures at our institution from August 1, 2018, to February 1, 2020. We created guidelines designed to limit opioid prescription doses based on data obtained from standardized postoperative telephone interviews. Three 6-month periods were evaluated: before guideline implementation, after guideline initiation, and after addressing barriers to guideline compliance. Targeted interventions to increase compliance included modification of electronic medical record defaults and provider educations. Differences in opioid weight-based doses prescribed, filled, and taken, as well as protocol adherence between the 3 timeframes were evaluated.

Results
A total of 1033 children underwent an outpatient procedure during the 1.5-year time frame. Phone call response rate was 72.22%. There was a significant sustained decrease in opioid doses prescribed (P < 0.0001), prescriptions filled (P = 0.009), and opioid doses taken (P = 0.001) after implementation, without subsequent increase in reported pain on postoperative phone call (P = 0.96). Protocol compliance significantly improved (62.39% versus 83.98%, P < 0.0001) after obstacles were addressed.

Conclusions
Implementation of a protocol limiting opioid prescribing after frequently performed pediatric general surgery procedures reduced opioids prescribed and taken postoperatively. Interventions that addressed barriers to application led to increased protocol compliance and sustained decreases in opioids prescribed and taken without a deleterious effect on pain control.
Mapping the Terrain of Faculty Development for Simulation
Authors: Gardner Aimee K. , Rodgers David L. , Steinert Yvonne , Davis Rachel , Condron Claire , Peterson Dawn Taylor , Rohra Anita , Viggers Sandra , Eppich Walter J. , Reedy Gabriel 
Published: January 2024

Abstract
Understanding what interventions and approaches are currently being used to improve the knowledge, skills, and effectiveness of instructors in simulation-based education is an integral step for carving out the future of simulation. The current study is a scoping review on the topic, to uncover what is known about faculty development for simulation-based education.
We screened 3259 abstracts and included 35 studies in this scoping review. Our findings reveal a clear image that the landscape of faculty development in simulation is widely diverse, revealing an array of foundations, terrains, and peaks even within the same zone of focus. As the field of faculty development in simulation continues to mature, we would hope that greater continuity and cohesiveness across the literature would continue to grow as well. Recommendations provided here may help provide the pathway toward that aim.
Understanding the Effects of Health Care Distance Simulation
Authors: Charnetski Matthew D. , Wawersik Dawn , Palaganas Janice C. , Duff Jonathan P. , Bailey Shannon K. T. , Ramachandra Geethanjali , Birido Nuha , Elkin Rachel , Nair Bindhu , Thurber Peter , Gross Isabel T. 
Published: January 2024

AbstractThe use of distance simulation has rapidly expanded in recent years with the physical distance requirements of the COVID-19 pandemic. With this development, there has been a concurrent increase in research activities and publications on distance simulation. The authors conducted a systematic review of the peer-reviewed distance health care simulation literature. Data extraction and a risk-of-bias assessment were performed on selected articles. Review of the databases and gray literature reference lists identified 10,588 titles for review. Of those, 570 full-text articles were assessed, with 54 articles included in the final analysis. Most of these were published during the COVID-19 pandemic (2020–2022). None of the included studies examined an outcome higher than a Kirkpatrick level of 2. Most studies only examined low-level outcomes such as satisfaction with the simulation session. There was, however, a distinction in studies that were conducted in a learning environment where all participants were in different locations (“distance only”) as compared with where some of the participants shared the same location (“mixed distance”). This review exclusively considered studies that focused solely on distance. More comparative studies exploring higher level outcomes are required to move the field forward.
Health Care Simulation in Person and at a Distance
Authors: Birido Nuha , Brown Kristen M. , Olmo Ferrer Diego , Friedland Richard , Bailey Shannon K. T. , Wawersik Dawn , Charnetski Matthew , Nair Bindhu , Kutzin Jared M. , Gross Isabel T. , Palaganas Janice C. 
Published: January 2024

AbstractDistance simulation is a method of health care training in which the learners and facilitators are in different physical locations. Although methods of distance simulation have existed in health care for decades, this approach to education became much more prevalent during the COVID-19 pandemic. This systematic review studies a subset of distance simulation that includes combined in-person and distance simulation elements, identified here as “mixed- distance simulation.” A review of the distance simulation literature identified 10,929 articles. Screened by inclusion and exclusion criteria, 34 articles were ultimately included in this review. The findings of this review present positive and negative aspects of mixed-distance simulation formats, a description of the most frequent configurations related to delivery, terminology challenges, as well as future directions including the need for faculty development, methodological rigor, and reporting details.
The Impact of Just-in-Time Simulation Training for Healthcare Professionals on Learning and Performance Outcomes: A Systematic Review
Authors: Patocka Catherine , Pandya Anjali , Brennan Erin , Lacroix Lauren , Anderson Ingrid , Ganshorn Heather , Hall Andrew K. 
Published: January 2024

Abstract
Although just-in-time training (JIT) is increasingly used in simulation-based health professions education, its impact on learning, performance, and patient outcomes remains uncertain. The aim of this study was to determine whether JIT simulation training leads to improved learning and performance outcomes. We included randomized or nonrandomized interventional studies assessing the impact of JIT simulation training (training conducted in temporal or spatial proximity to performance) on learning outcomes among health professionals (trainees or practitioners). Of 4077 citations screened, 28 studies were eligible for inclusion. Just-in-time training simulation training has been evaluated for a variety of medical, resuscitation, and surgical procedures. Most JIT simulation training occurred immediately before procedures and lasted between 5 and 30 minutes. Despite the very low certainty of evidence, this systematic review suggests JIT simulation training can improve learning and performance outcomes, in particular time to complete skills. There remains limited data on better patient outcomes and collateral educational effects.
Bibliometric Analysis on Geriatric Rehabilitation in Scopus Database (1948-2022)
Authors: Azizan Azliyana , Azmi Alia , Yusof Mohd Yusmiaidil Putera Mohd 
Published: January 2024

Background:
Aging presents both challenges and opportunities. It will increase demand for health care to restore function or enhance residual functional capability and improving the quality of life in older people. The objective of the present investigation was to offer perspectives into the worldwide research production concerning the geriatric rehabilitation.

Methods:
A bibliometric analysis was conducted utilizing the Scopus database covering the period of 1948 through 2022. Several methods and instruments were employed to scrutinize and illustrate the data.

Results:
The search strategy found 6630 articles. The United States was the most active country with the highest number of coauthors. VA Medical Center was the most active organization with Becker the most active author in Geriatric Rehabilitation research with 40 articles from Germany, followed by Hauer and Trabucchi with 39 and 37 articles, respectively. The most frequent words in this field were “rehabilitation, elderly, geriatrics, stroke, hip fracture, aged, dementia and activities of daily living.” The most influential articles were specified. In terms of journals, BMC Geriatrics was the most active, while the most cited article was “Gait Variability and Fall Risk in Community-Living Older Adults: A 1-Year Prospective Study” by Hausdorff in 2001.

Conclusions:
Geriatric rehabilitation has received special attention in the last decade. Current studies highlight the importance and understanding of the unique needs of older adults. The core for this area that includes impairment related to physical, cognitive, and functional needs is considered. Hence, health care practitioners and scholars must prioritize geriatric rehabilitation as a specialty and comprehend the optimal strategy for supporting elderly individuals to maintain their independence as much as feasible.
Feasibility and Clinician Perspectives of the Visual Symptoms and Signs Screen
Authors: Roberts Pamela S. , Wertheimer Jeffrey , Ouellette Debra , Hreha Kimberly , Watters Kelsey , Fielder Jaimee , Graf Min Jeong P. , Weden Kathleen M. , Rizzo John Ross 
Published: January 2024

Background:The Visual Symptoms and Signs Screen (V-SASS) is a tool to identify vision deficits and facilitate referrals to vision specialists. The study objectives were to determine feasibility and clinician perspectives of the V-SASS.Methods:Prospective, multisite study with 141 new-onset stroke participants. After V-SASS administration, feasibility and predictive success were assessed.Results:The V-SASS identified vision symptoms and signs with high feasibility (>75%). Of those who screened positive, 93.1% had deficits in visual function or functional vision.Conclusions:The V-SASS was found to be feasible in multiple settings and accurately identify vision deficits and appropriately trigger vision referrals.
Persistence and Free Chlorine Disinfection of Human Coronaviruses and Their Surrogates in Water
Authors: Zhang Mengyang , Leong Michelle Wei , Mitch William A. , Blish Catherine A. , Boehm Alexandria 
Published: January 2024

AbstractThe COVID-19 pandemic illustrates the importance of understanding the behavior and control of human pathogenic viruses in the environment. Exposure via water (drinking, bathing, and recreation) is a known route of transmission of viruses to humans, but the literature is relatively void of studies on the persistence of many viruses, especially coronaviruses, in water and their susceptibility to chlorine disinfection. To fill that knowledge gap, we evaluated the persistence and free chlorine disinfection of human coronavirus OC43 (HCoV-OC43) and its surrogates, murine hepatitis virus (MHV) and porcine transmissible gastroenteritis virus (TGEV), in drinking water and laboratory buffer using cell culture methods. The decay rate constants of human coronavirus and its surrogates in water varied depending on virus and water matrix. In drinking water prior to disinfectant addition, MHV showed the largest decay rate constant (2.25 day-1) followed by HCoV-OC43 (0.99 day-1) and TGEV (0.65 day-1); while in phosphate buffer, HCoV-OC43 (0.51 day-1) had a larger decay rate constant than MHV (0.28 day-1) and TGEV (0.24 day-1). Upon free chlorine disinfection, the inactivation rates of coronaviruses were independent of free chlorine concentration and not affected by water matrix, though they still varied between viruses. TGEV showed the highest susceptibility to free chlorine disinfection with the inactivation rate constant of 113.50 mg-1min-1L, followed by MHV (81.33 mg-1min-1L) and HCoV-OC43 (59.42 mg-1min-1L).ImportanceThis study addresses an important knowledge gap on enveloped virus persistence and disinfection in water. Results have immediate practical applications for shaping evidence-based water policies, particularly in the development of disinfection strategies for pathogenic virus control.
Ecological lifestyle and gill slit height across sharks
Authors: Vander Wright Wade J. , Bigman Jennifer S. , Iliou Anthony S. , Dulvy Nicholas K. 
Published: January 2024

AbstractMorphology that is linked to metabolic rate – metabolic morphology – provides broad comparative insights into the physiological performance and ecological function of species. However, some metabolic morphological traits, such as gill surface area, require costly and lethal sampling. Measurements from anatomically-accurate drawings, such as those in field guides, offer the opportunity to understand physiological and ecological relationships without the need for physical, lethal sampling. Here, we assess the relationship between the metabolic physiology and ecology of nearly all extant sharks. Specifically, we examine the relationship between gill slit height and each of the three traits that comprise ecological lifestyle: activity, maximum size, and depth. We find that gill slit heights are positively related to activity (measured by the aspect ratio of the caudal fin) and maximum size but negatively related to depth. We also show that gill slit height is best explained by the suite of ecological lifestyle traits rather than any single trait. These results suggest that more active, larger, and shallower species have higher metabolic demands and that these greater metabolic demands can be estimated from external morphological and ecological traits. Our work demonstrates that meaningful ecophysiological relationships can be revealed through measurable metabolic morphological traits from anatomically-accurate drawings.
High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes
Authors: Locke Timothy M. , Fields Rose , Gizinski Hayden , Otto George M. , Shechner David M. , Berg Matthew D. , Villen Judit , Sancak Yasemin , Schweppe Devin 
Published: January 2024

SummaryCalcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by lack of high-throughput, unbiased, and quantitative methods to identify proteins-calcium engagement. To address this, we adapted protein thermostability assays in the budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. These data revealed calcium engagement of novel signaling hubs and cellular processes, including metabolic enzymes and the spliceosome. Cross-species comparison of calcium-protein engagement and mutagenesis experiments identified residue-specific cation engagement, even within well-known EF-hand domains. Additionally, we found that the dienoyl-CoA reductase DECR1 binds calcium at physiologically-relevant concentrations with substrate-specific affinity, suggesting direct calcium regulation of mitochondrial fatty acid oxidation. These unbiased, proteomic analyses of calcium effectors establish a key resource to dissect cation engagement and its mechanistic effects across multiple species and diverse biological processes.
Better abstract or concrete, narrating or not: optimal strategies for the communication of innovation
Authors: Cardamone Ernesto , Miceli Gaetano , Raimondo Maria Antonietta 
Published: January 2024

PurposeThis paper investigates how two characteristics of language, abstractness vs concreteness and narrativity, influence user engagement in communication exercises on innovation targeted to the general audience. The proposed conceptual model suggests that innovation fits well with more abstract language because of the association of innovation with imagination and distal construal. Moreover, communication of innovation may benefit from greater adherence to the narrativity arc, that is, early staging, increasing plot progression and climax optimal point. These effects are moderated by content variety and emotional tone, respectively.Design/methodology/approachBased on a Latent Dirichlet allocation (LDA) application on a sample of 3225 TED Talks transcripts, the authors identify 287 TED Talks on innovation, and then applied econometric analyses to test the hypotheses on the effects of abstractness vs concreteness and narrativity on engagement, and on the moderation effects of content variety and emotional tone.FindingsThe authors found that abstractness (vs concreteness) and narrativity have positive effects on engagement. These two effects are stronger with higher content variety and more positive emotional tone, respectively.Research limitations/implicationsThis paper extends the literature on communication of innovation, linguistics and text analysis by evaluating the roles of abstractness vs concreteness and narrativity in shaping appreciation of innovation.Originality/valueThis paper reports conceptual and empirical analyses on innovation dissemination through a popular medium – TED Talks – and applies modern text analysis algorithms to test hypotheses on the effects of two pivotal dimensions of language on user engagement.