bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dispersal and influences on movement forAnoplophora glabripenniscalculated from individual mark‐recapture
Authors: Bancroft J.S. , Smith M.T. 
Published: July 2005

AbstractWe conducted an individual mark‐release‐recapture experiment on the beetle,Anoplophora glabripennisMotchulsky (Coleoptera: Cerambycidae). This invasive beetle has been introduced from Asia to Europe and North America and poses a serious threat to several important species of tree. Eradication efforts may benefit from knowledge of dispersal behaviour. Trees were cut and held to determine emergence rate ofA. glabripennis. Unique marks were painted onto 912 beetles released into a group of 165 trees in Gansu, China. Data on subsequent sightings of beetles were used in a truncated diffusion model to calculate flight distances. Characteristics of the trees and climatic information were used in statistical tests for influence on movement. A total of 2245 sightings of beetles were observed and 29% of marked beetles were resighted. The scanning technique using binoculars was 90% effective in finding beetles and provided 81% accuracy for determining the sex of the beetles. Experimental manipulation of density quantified howA. glabripenniscongregated on unoccupied trees and were repulsed from crowded hosts. The seasonal emergence rate of adults declined exponentially from July 20 to August 5. The results suggestedA. glabripennisfly to nearby host trees at a rate of 34% per day. Median flight distance was estimated at 20 m per day. Statistical analysis with a generalized linear model tested the beetle's propensity to leave a tree and distance of flight. Generally, beetle movement showed a significant response to beetle density, weather conditions, beetle size, and tree size, in that order. The techniques developed here improve on previous recapture techniques to quantify dispersal and can be useful for analysing populations of other organisms.
Overexpression of Shp2 tyrosine phosphatase is implicated in leukemogenesis in adult human leukemia
Authors: Xu Rongzhen , Yu Yingzi , Zheng Shu , Zhao Xiaoying , Dong Qinghua , He Zhiwen , Liang Yun , Lu Qinghua , Fang Yongmin , Gan Xiaoxian , Xu Xiaohua , Zhang Suzhan , Dong Qi , Zhang Xiaohong , Feng Gen-Sheng 
Published: July 2005

AbstractShp2 tyrosine phosphatase plays a critical role in hematopoiesis, and dominant active mutations have been detected in the human gene PTPN11, encoding Shp2, in child leukemia patients. We report here that although no such mutations were detected in 44 adult leukemia patients screened, Shp2 expression levels were significantly elevated in primary leukemia cells and leukemia cell lines, as compared with normal hematopoietic progenitor cells. The Shp2 protein amounts correlated well with the hyperproliferative capacity but were inversely associated with the differentiation degree of leukemia cells. Suppression of Shp2 expression induced apoptosis and inhibition of leukemic cell clonogenic growth. Notably, the majority of Shp2 was preferentially localized to the plasma membrane and was constitutively phosphorylated on tyrosine in leukemia cells, and also in normal hematopoietic cells following mitogenic stimulation. Based on these results, we propose that aberrantly increased expression of Shp2 may contribute, collaboratively with other factors, to leukemogenesis.
Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3
Authors: Penna Giuseppe , Roncari Andrea , Amuchastegui Susana , Daniel Kenn C. , Berti Emilio , Colonna Marco , Adorini Luciano 
Published: July 2005

Abstract1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is a secosteroid hormone that renders dendritic cells (DCs) tolerogenic, favoring the induction of regulatory T cells. Induction of DCs with tolerogenic properties by 1,25(OH)2D3 is associated with increased selective expression of immunoglobulin-like transcript 3 (ILT3), suggesting its involvement in the immunoregulatory properties of this hormone. Here we show an in vivo correlate of the increased ILT3 expression on DCs in healing psoriatic lesions following topical treatment with the 1,25(OH)2D3 analog calcipotriol. Analysis of DC subsets reveals a differential regulation of ILT3 expression by 1,25(OH)2D3, with a marked up-regulation in myeloid DCs but no effect on its expression by plasmacytoid DCs. A regulatory role for ILT3 expressed on DCs is indicated by the increased interferon-γ (IFN-γ) secretion promoted by anti-ILT3 addition to cultures of DCs and T cells, but this effect is blunted in 1,25(OH)2D3-treated DCs, suggesting ILT3-independent mechanisms able to regulate T-cell activation. Although ILT3 expression by DCs is required for induction of regulatory T cells, DC pretreatment with 1,25(OH)2D3 leads to induction of CD4+Foxp3+ cells with suppressive activity irrespective of the presence of neutralizing anti-ILT3 monoclonal antibody (mAb), indicating that ILT3 expression is dispensable for the capacity of 1,25(OH)2D3-treated DCs to induce regulatory T cells.
Genetic Services for Familial Cancer Patients: A Follow-Up Survey of National Cancer Institute Cancer Centers
Authors: Epplein Meira , Koon Ksenia P. , Ramsey Scott D. , Potter John D. 
Published: July 2005

Purpose Anecdotal reports suggest that the volume of services offered to individuals concerned with hereditary cancer risk has increased substantially in recent years. As a follow-up to our 1993 survey, we sought to determine how the scope and volume of genetic services has changed between 1993 and 2002. Methods We surveyed the 61 National Cancer Institute–designated cancer centers in operation in 2002 using an updated version of the questionnaire from 1993. Analysis included frequencies and summary statistics. Results The majority of cancer centers responding (46 of 56 centers; 82.1%) provided some genetic services for evaluation of familial cancer, which is a higher proportion than in 1993 (50%; P < .01). Almost all centers (42 of 46 centers; 91.3%) provided services not only to cancer patients and their families, but also to individuals concerned with risk, which is a change (P = .01) from 1993, when 64.7% of centers offered such services. In addition, increases have been found for most other measures of services rendered for familial genetic services. Conclusion As public awareness of cancer susceptibility genes has grown markedly in recent years, the demand has also grown for genetic services to assess familial cancer risk. Major deleterious genetic mutations are rare, and much of the current research in genetic variation focuses on higher prevalence variants that carry lower risks. This may suggest that testing for mutations will move from genetics clinics to primary care and specialty practices. Thus, it is unclear whether the scope and volume of cancer center genetics services will continue to grow as rapidly as they have over the last decade.
Immunohistochemistry Identifies Carriers of Mismatch Repair Gene Defects Causing Hereditary Nonpolyposis Colorectal Cancer
Authors: Stormorken Astrid T. , Bowitz-Lothe Inger Marie , Norèn Tove , Kure Elin , Aase Steinar , Wijnen Juul , Apold Jaran , Heimdal Ketil , Møller Pål 
Published: July 2005

Purpose Hereditary nonpolyposis colorectal cancer (HNPCC) may be caused by mutations in mismatch repair (MMR) genes. The aim of this study was to validate immunohistochemistry and family history as prescreening tools to predict germline mutations in MLH1, MSH2, and MSH6. Patients and Methods Pedigrees from 250 families were extended, cancer diagnoses were verified, and families were classified according to the Amsterdam and the Bethesda criteria. Tumor specimens were examined with immunohistochemistry for the presence of MLH1, MSH2, and MSH6 proteins. Mutation analyses were performed in blood samples from the same patients. Results Blood samples from affected index persons in 181 families and tumor specimens from 127 of the affected index persons were obtained. Thirty tumors lacked one or more gene products. Sensitivity of immunohistochemistry to detect mutation carriers was 100%, specificity was 82%, and positive predictive value was 85%. Sensitivities, specificities, and positive predictive values for the Amsterdam criteria were 82%, 8%, and 45%, respectively, and for the Bethesda criteria were 100%, 0%, and 48%, respectively. Distribution of mutations was MLH1 = 4, MSH2 = 11, and MSH6 = 4. Conclusion Wide clinical criteria to select HNPCC kindreds, followed by immunohistochemistry of tumor material from one affected person in each family, had high sensitivity and specificity to predict MMR mutations.
Significant Effect of Capecitabine on the Pharmacokinetics and Pharmacodynamics of Warfarin in Patients With Cancer
Authors: Camidge Ross , Reigner Bruno , Cassidy Jim , Grange Susan , Abt Markus , Weidekamm Erhard , Jodrell Duncan 
Published: July 2005

PurposeClinical cases of capecitabine and other fluorouracil-based chemotherapies potentiating the effects of coumarin derivatives have been reported. This study assessed the influence of capecitabine on the pharmacokinetics (PK) and pharmacodynamics (PD) of warfarin.Patients and MethodsFour patients with advanced/metastatic cancer completed the study, receiving a single oral dose of 20 mg warfarin before the start of standard capecitabine treatment (day 1), and again during the third cycle of capecitabine (day 61). PK parameters of warfarin and capecitabine and PD parameters of warfarin were assessed on days 1 and 61.ResultsDuring capecitabine treatment, the area under the plasma concentration time curve from 0 to infinity (AUC0-∞) of S-warfarin increased by 57% (90% CI, 32% to 88%) with a 51% prolongation of the elimination half-life (t1/2; 90% CI, 32% to 74%). Exposure to R-warfarin was not significantly affected. Plasma concentrations of capecitabine and its metabolites were not influenced by warfarin. During capecitabine treatment, the effect of warfarin on the baseline corrected AUC of the International Normalized Ratio (INR) increased by 2.8 times (90% CI, 1.33 to 5.70), with the maximum observed INR value almost doubling. Because of the administration of vitamin K to some patients with elevated INRs, these figures are likely to underestimate the true PD effect. Mean baseline factor VII levels dropped while on capecitabine therapy, potentially contributing to the observed PD interaction, though this effect did not reach statistical significance.ConclusionThere is a significant pharmacokinetic interaction between capecitabine and S-warfarin, resulting in exaggerated anticoagulant activity. Patients receiving warfarin anticoagulant therapy concomitantly with capecitabine should have their INR closely monitored and warfarin doses adjusted accordingly.
Sentinel Lymphadenectomy Does Not Increase the Incidence of In-Transit Metastases in Primary Melanoma
Authors: Kang John C. , Wanek Leslie A. , Essner Richard , Faries Mark B. , Foshag Leland J. , Morton Donald L. 
Published: July 2005

Purpose Recent reports by European investigators suggest that sentinel lymphadenectomy (SLND), a mainstay of melanoma diagnosis and treatment planning, increases the risk of in-transit metastasis (ITM) and should be abandoned. This study compared the incidence of ITM after wide local excision (WLE), WLE plus SLND (SLND), or WLE plus elective lymphadenectomy (ELND) for primary melanoma. Patients and Methods Review of our prospective database identified 4,412 patients who underwent WLE (n = 2,771), SLND (n = 1,016), or ELND (n = 625) for stage I/II melanoma (1971 through 2002). The incidence of ITM overall and as a first recurrence was examined before and after computerized prognostic matching of treatment groups. Intergroup statistical comparisons used χ2 analysis and log-rank test. Results The incidence of ITM increased with Breslow depth, Clark level, and T stage. Although overall incidence of ITM was significantly higher (P = .0008) after ELND (6.56%) versus WLE (3.36%) or SLND (3.64%), the ELND group had higher risk primaries. Treatment groups matched by T stage (1,875 patients; 625 per group) or by age, sex, Breslow depth, and primary location (1,680 patients; 560 per group), showed no significant differences in ITM overall or as a first recurrence. Conclusion There is no relationship between SLND and ITM. Recent reports to the contrary reflect analysis of significantly smaller cohorts not matched for confounding variables such as T stage. The phase III Multicenter Selective Lymphadenectomy Trial will definitively settle the issue; until then, use of SLND, the most accurate staging procedure for early-stage melanoma, should continue.
The Interaction of a New Motor Skill and an Old One: H-Reflex Conditioning and Locomotion in Rats
Authors: Chen Yi , Chen Xiang Yang , Jakeman Lyn B. , Schalk Gerwin , Stokes Bradford T. , Wolpaw Jonathan R. 
Published: July 2005

New and old motor skills can interfere with each other or interact in other ways. Because each skill entails a distributed pattern of activity-dependent plasticity, investigation of their interactions is facilitated by simple models. In a well characterized model of simple learning, rats and monkeys gradually change the size of the H-reflex, the electrical analog of the spinal stretch reflex. This study evaluates in normal rats the interactions of this new skill of H-reflex conditioning with the old well established skill of overground locomotion.In rats in which the soleus H-reflex elicited in the conditioning protocol (i.e., the conditioning H-reflex) had been decreased by down-conditioning, the H-reflexes elicited during the stance and swing phases of locomotion (i.e., the locomotor H-reflexes) were also smaller. Similarly, in rats in which the conditioning H-reflex had been increased by up-conditioning, the locomotor H-reflexes were also larger.Soleus H-reflex conditioning did not affect the duration, length, or right/left symmetry of the step cycle. However, the conditioned change in the stance H-reflex was positively correlated with change in the amplitude of the soleus locomotor burst, and the correlation was consistent with current estimates of the contribution of primary afferent input to the burst.Although H-reflex conditioning and locomotion did not interfere with each other, H-reflex conditioning did affect how locomotion was produced: it changed soleus burst amplitude and may have induced compensatory changes in the activity of other muscles. These results illustrate and clarify the subtlety and complexity of skill interactions. They also suggest that H-reflex conditioning might be used to improve the abnormal locomotion produced by spinal cord injury or other disorders of supraspinal control.
Swelling behavior of semi‐interpenetrating polymer network hydrogels composed of poly(vinyl alcohol) and poly(acrylamide‐co‐sodium methacrylate)
Authors: Mohan Y. Murali , Murthy P. S. Keshava , Sreeramulu J. , Raju K. Mohana 
Published: July 2005

AbstractInterpenetrating polymer network (IPN) hydrogels based on poly(vinyl alcohol) (PVA) and poly(acrylamide‐co‐sodium methacrylate) poly(AAm‐co‐SMA) were prepared by the semi IPN method. These IPN hydrogels were prepared by polymerizing aqueous solution of acrylamide and sodium methacrylate, using ammonium persulphate/N,N,N1,N1‐tetramethylethylenediamine (APS/TMEDA) initiating system and N,N1‐methylene‐bisacrylamide (MBA) as a crosslinker in the presence of a host polymer, poly(vinyl alcohol). The influence of reaction conditions, such as the concentration of PVA, sodium methacrylate, crosslinker, initiator, and reaction temperature, on the swelling behavior of these IPNs was investigated in detail. The results showed that the IPN hydrogels exhibited different swelling behavior as the reaction conditions varied. To verify the structural difference in the IPN hydrogels, scanning electron microscopy (SEM) was used to identify the morphological changes in the IPN as the concentration of crosslinker varied. In addition to MBA, two other crosslinkers were also employed in the preparation of IPNs to illustrate the difference in their swelling phenomena. The swelling kinetics, equilibrium water content, and water transport mechanism of all the IPN hydrogels were investigated. IPN hydrogels being ionic in nature, the swelling behavior was significantly affected by environmental conditions, such as temperature, ionic strength, and pH of the swelling medium. Further, their swelling behavior was also examined in different physiological bio‐fluids. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 302–314, 2005
A new composite from cellulose industrial waste and elastomeric polyurethane
Authors: Silva M. C. , Silva G. G. 
Published: July 2005

AbstractCellulose fiber waste from the white cellulose pulp purification process produced by the company Nipo Cellulose‐Brazil (CENIBRA) was used in the preparation of composites with polyurethane. An elastomeric polyurethane based on polyether poly(tetramethylene glycol) and 4,4‐diphenylmethane diisocyanate was used as polymeric matrix. Structural, thermal, and morphological characterization of the composites was carried out by infrared spectroscopy, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The results indicate that interfacial interaction between the cellulose fiber residue and the polyurethane matrix occurs and that cellulose waste is a promising reinforcement for polyurethane composites. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 336–340, 2005
Synthesis and characterization of side‐chain liquid‐crystalline elastomers containing cholesterol
Authors: He Xiao‐Zhi , Xiao Ling‐Jiu , Zhang Bao‐Yan , Xiao Wen‐Qiang 
Published: July 2005

AbstractA series of new smectic and cholesteric liquid‐crystalline elastomers were prepared by graft polymerization of mesogenic monomer with the chiral and nonmesogenic crosslinking agent using polymethylhydrosiloxane as backbone. The chemical structures of the monomers and polymers obtained were confirmed by Fourier transform infrared and proton nuclear magnetic resonance spectra. The mesomorphic properties were investigated by differential scanning calorimetry, polarizing optical microscopy, and X‐ray diffraction measurements. M1 showed cholesteric phase during the heating and the cooling cycle. Polymer P1, elastomers P2 and P3 exhibited smectic phase, elastomers P4P6 showed chiral smectic C phase, P7 showed cholesteric phase, and P8 displayed stress‐induced birefringence. The elastomers containing less than 15 mol % M2 displayed elasticity and reversible phase transition with wide mesophase temperature ranges. Experimental results demonstrated that the glass transition temperatures, the isotropization temperatures, and the mesophase temperature ranges decreased with increasing content of the crosslinking unit. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 383–390, 2005
Anatomy that must be taught to a medical undergraduate: An interview‐based survey in an Indian medical school
Authors: Nayak Satheesha , Ramnarayan K. , Somayaji S.N. 
Published: July 2005

AbstractConfusion still exists about the anatomy to be taught to the medical undergraduate. We did an interview‐based survey at the Melaka Manipal Medical College in Manipal, India, to try to evaluate the quantum of anatomy that should be taught to the medical undergraduate. The results suggest that excluding trivia and making anatomy more clinically oriented would be advantageous. A hybrid approach to anatomy including both problem‐based learning and discipline‐based curricula would be a better option than the regional or systemic approaches. Anat Rec (Part B: New Anat) 285B:16–18, 2005. © 2005 Wiley‐Liss, Inc.
DNA methylation in mammalian development and disease
Authors: Dean Wendy , Lucifero Diana , Santos Fátima 
Published: July 2005

AbstractEpigenetic modification of the cytosine base of DNA by its methylation introduced the possibility that beyond the inherent information contained within the nucleotide sequence there was an additional layer of information added to the underlying genetic code. DNA methylation has been implicated in a wide range of biological functions, including an essential developmental role in the reprogramming of germ cells and early embryos, the repression of endogenous retrotransposons, and a generalized role in gene expression. Special functions of DNA methylation include the marking of one of the parental alleles of many imprinted genes, a group of genes essential for growth and development in mammals with a unique parent‐of‐origin expression pattern, a role in stabilizing X‐chromosome inactivation, and centromere function. In this regard, it is not surprising that errors in establishing or maintaining patterns of methylation are associated with a diverse group of human diseases and syndromes. Birth Defects Research (Part C) 75:98–111, 2005. © Wiley‐Liss, Inc.
Epigenetic deregulation of genomic imprinting in human disorders and following assisted reproduction
Authors: Arnaud Philippe , Feil Robert 
Published: July 2005

AbstractImprinted genes play important roles in the regulation of growth and development, and several have been shown to influence behavior. Their allele‐specific expression depends on inheritance from either the mother or the father, and is regulated by “imprinting control regions” (ICRs). ICRs are controlled by DNA methylation, which is present on one of the two parental alleles only. These allelic methylation marks are established in either the female or the male germline, following the erasure of preexisting DNA methylation in the primordial germ cells. After fertilization, the allelic DNA methylation at ICRs is maintained in all somatic cells of the developing embryo. This epigenetic “life cycle” of imprinting (germline erasure, germline establishment, and somatic maintenance) can be disrupted in several human diseases, including Beckwith‐Wiedemann syndrome (BWS), Prader‐Willi syndrome (PWS), Angelman syndrome and Hydatidiform mole. In the neurodevelopmental Rett syndrome, the way the ICR mediates imprinted expression is perturbed. Recent studies indicate that assisted reproduction technologies (ART) can sometimes affect the epigenetic cycle of imprinting as well, and that this gives rise to imprinting disease syndromes. This finding warrants careful monitoring of the epigenetic effects, and absolute risks, of currently used and novel reproduction technologies. Birth Defects Research (Part C) 75: 81–97, 2005. © 2005 Wiley‐Liss, Inc.
Mechanisms and consequences of paternally‐transmitted chromosomal abnormalities
Authors: Marchetti Francesco , Wyrobek Andrew J. 
Published: July 2005

AbstractPaternally‐transmitted chromosomal damage has been associated with pregnancy loss, developmental and morphological defects, infant mortality, infertility, and genetic diseases in the offspring, including cancer. There is epidemiological evidence linking paternal exposure to occupational or environmental agents with an increased risk of abnormal reproductive outcomes. There is also a large body of literature on germ cell mutagenesis in rodents showing that treatment of male germ cells with mutagens has dramatic consequences on reproduction, producing effects such as those observed in human epidemiological studies. However, we know very little about the etiology, transmission, and early embryonic consequences of paternally‐derived chromosomal abnormalities. The available evidence suggests that: 1) there are distinct patterns of germ cell–stage differences in the sensitivity of induction of transmissible genetic damage, with male postmeiotic cells being the most sensitive; 2) cytogenetic abnormalities at first metaphase after fertilization are critical intermediates between paternal exposure and abnormal reproductive outcomes; and 3) there are maternal susceptibility factors that may have profound effects on the amount of sperm DNA damage that is converted into chromosomal aberrations in the zygote and that directly affect the risk for abnormal reproductive outcomes. Birth Defects Research (Part C) 75:112–129, 2005. © 2005 Wiley‐Liss, Inc.