Enhanced Phytoremediation and Physicochemical Parameters of Crude Oil Polluted Soil Using Pseudomonas fluorescens and Bacillus substilis
Authors: Olufunmilayo Williams Janet , Emmanuel Jaja Soba , Renna Nrior Renna 
Published: December 2023

There have been worry over the Niger Delta's environmental contamination. Bacteria and other microorganisms have shown to be very helpful in the breakdown of hydrocarbons generated from petroleum. The goal of this research is to use elbow bufallow grass and sedge plants for phytoremediation of soil affected by crude oil. Standard microbiological techniques were applied to the contaminated soil once it was gathered. Using a hand auger, contaminated soil samples were taken twice a month for three months from two separate locations in Rivers State at two distinct depths: 0–15 cm and 15–30 cm. The following physicochemical parameters of samples were analysed using Standard Laboratory Procedures: pH, Temperature, Nitrogen, Phosphorus, Potassium available in the polluted soil and the total hydrocarbon content (THC). Two plant species common in the Ogoni region of Rivers state, i.e. Sedge plant (Schoenoplectus), Elbow buffalo grass (Panicum subalbidum) were used for phytoremediation monitoring. A combination of treatment consisting of the application of Pseudomonas fluorescens Bacillus substilis, Panicum subalbidum and Schoenoplectus senegalensis was evaluated during 28 days of remediation. Each pot contained crude oil mixture in the soil as a sole source of carbon and energy. THB counts ranged from 2.35 to 4.15 cfu/g. The statistical analysis revealed that there was no significant difference (p>0.05) in the total heterotrophic bacteria counts between the samples. HUB counts range from 0.7 to 1.45cfu/g. The total bacterial population counts obtained from soil sample during bioremediation monitoring ranged from 17+1.41 (CS+PAN) to 40+1.412cfu/g (CS+PSE+BAC+PAN) in Day 1. Results of Day 14 range from 13+1.41 (CS+PAN) to 35.5+3.542cfu/g (CS+BAC+SCH). Results of Day 28 ranged from 8.5+0.71 (CS+PAN) to 27+1.412cfu/g (CS+PSE+BAC+PAN). The presence of microbial activity was determined by the enumeration and isolation of total heterotrophic and hydrocarbon utilizing bacteria. The results of physicochemical parameters before bioremediation is as follows: pH (5.43), Temperature (27oC), Electrical conductivity (9), Moisture content (7.80%), Total organic carbon (0.93%), Soil organic matter (1.60%), Nitrogen (56.695mg/kg), Phosphorus (0.621mg/kg), Potassium (7.125mg/kg) and Total Hydrocarbon content (700mg/kg). Results revealed amount of soil hydrocarbon removed and percentage (%) Bioremediation remediated after 28 days of monitoring to be higher in set up with CS+PSE+SCH (3454mg/kg; 85.28%) and lowest in set up with US+SCH (434mg/kg: 62%) and the amount of root hydrocarbon content removed and percentage (%) Bioremediation remediated after 28 days of monitoring to be higher in set up with CS+BAC+SCH (632Mg/kg; 15.6%) and lowest in set up with US+SCH (12.2mg/kg; 1.74%).
Three (3) most occurring hydrocarbon utilizing bacterial isolates were isolated and identified culturally and phenotypically from the soil samples these bacteria isolates were confirmed to be Peudomonas, Priestia megaterium and Bacillus spp molecularly via sequencing of the 16SrRNA gene. The most common bacteria isolated were Bacillus spp at a dilution of 104. This research revealed and recommend that Panicum subalbidum as a suitable plant species for phytoremediation of crude oil-contaminated soil.