bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimal Contract Under Endogenous Platform Services: Implications of Tax and Advertising
Authors: Poddar Sangita , Banerjee (Chatterjee) Tanmoyee , Banerjee Swapnendu 
Published: November 2023

The paper evaluates the contracting problem between a platform and a seller under information asymmetry where the seller holds private information about his/her cost for product quality. Price per product is influenced by seller’s product quality and platform’s service quality. Cost-sharing contract is more desirable as it induces a higher level of qualities and generates higher profit for the platform compared to revenue-sharing contract. The product quality and platform’s service quality vary negatively with the ad-valorem tax imposed on price of the product. We then introduce advertising in our model and observe that the level of advertising is lower under information asymmetry. JEL Classifications: D86, L21, M37
Design of a High Precision Multichannel 3D Bioprinter
Authors: Li Jintao , Zhang Bin , Luo Yichen , Yang Huayong 
Published: November 2023

AbstractThree-dimensional (3D) printing technology is expected to solve the organ shortage problem. However, owing to the accuracy limitations, it is difficult for the current bioprinting technology to achieve an accurate control of the spatial position and distribution of a single cell or single component droplet. In this study, to accurately achieve the directional deposition of different cells and biological materials in the spatial position for the construction of large transplantable tissues and organs, a high-precision multichannel 3D bioprinter with submicron-level motion accuracy is designed, and concurrent and synergistic printing methods are proposed. Based on the high-precision motion characteristics of the gantry structure and the requirements of concurrent and synergistic printing, a 3D bioprinting system with a set of 6 channels is designed to achieve six-in-one printing. Based on the Visual C++ environment, a control system software that integrates the programmable multi-axis controller (PMAC) motion, pneumatic, and temperature control subsystems was developed and designed. Finally, based on measurements and experiments, the 3D bioprinter and its control system was verified to fulfil the requirements of multichannel, concurrent, and synergistic printing with submicron-level motion accuracy, significantly shortening the printing time and improving the printing efficiency. This study not only provides an equipment basis for printing complex heterogeneous tissue structures, but also improves the flexibility and functionality of bioprinting, and ultimately makes the construction of complex multicellular tissues or organs possible.
Investigation of mechanical properties and elucidation of factors affecting wood-based structural panels under embedment stress with a circular dowel II: detailed observation for plywood and OSB using DIC and CT scanning
Authors: Sudo Ryutaro , Aoki Kenji 
Published: November 2023

AbstractAlthough embedment properties are vital to timber engineering, the behavior and strain distributions in wood-based panels have not been clarified in detail. Our early studies suggested four possible causes of failure behavior and strain distribution: (i) two types of failure behavior (in-plane and out-of-plane failure); (ii) enlargement of the stress-spreading range with increasing load step; (iii) reduction of the stress-spreading range (normalized by dowel diameter) with increasing dowel diameter; and (iv) preferential stress spreading in the vertical and horizontal directions along the strong and weak-axis specifications, respectively. However, these hypotheses were not supported by actual observations. The present study aims to observe and clarify the surface strain distribution via digital image correlation and the internal failure behavior via computed tomography scanning. Most results of the wood-based panel specimens (plywood and oriented strand board) did not contradict the above hypotheses. The failure behaviors of plywood and oriented strand board are likely determined by the direction of the veneer fibers and the layer’s position, respectively. Within the strong axial layer of plywood, fibers on both sides of the dowel were densified by fibers dissociated immediately above the dowel, whereas the weak axial layer in plywood was deformed like a timber under partial compression perpendicular to the grain. In contrast, oriented strand board under an embedding stress exhibited a circularly distributed strain and a dispersed void area in its outer layer. Densification was observed only in the inner layer.
Comprehensive exploration of the involvement of cuproptosis in tumorigenesis and progression of neuroblastoma
Authors: Zhou Rui , Huang Dongmei , Fu Wen , Shu Fangpeng 
Published: November 2023

Abstract
Background
Copper-induced cell death, or “cuproptosis,” as an apoptotic process, has recently received much attention in human diseases. Recent studies on cuproptosis have provided novel insights into the pathogenesis of various diseases, especially cancers. However, the association between neuroblastoma (NB) and cuproptosis in terms of their clinical outcomes, tumorigenesis, and treatment response remains unclear.

Methods
To determine the role of cuproptosis in NB tumorigenesis and progression, this study employed a systematic technique to explore the characteristic patterns of 10 key cuproptosis-related genes (CUGs) in NB. Consensus clustering analysis of the TARGET and GEO databases divided the NB patients into two subgroups that showed different clinicopathological attributes, molecular patterns, survival outcomes, disease-associated pathways, tumor immune microenvironment (TIME) features, and treatment responses. Moreover, a cuproptosis scoring scheme was established, which divided the patients with NB into two groups with high scores and low scores as per the median score. Furthermore, this research developed a nomogram and risk signature on the basis of this cuproptosis score to better elucidate its function in predicting NB prognosis. In vitro experiments were carried out using Transwell Assay, HLECs tube formation assay, Colony formation assay, Western Blotting Assay, Immunohistochemical (IHC) Staining, Immunofluorescence (IF) Staining and Flow Cytometry Analysis.

Results
The results demonstrated that the established cuproptosis score and prediction model could effectively distinguish between the individuals in low and high-risk groups and had a high predictive value. Lastly, bioinformatics analysis and in vitro experiments enabled the identification of PDHA1, a key CUG, which was involved in both DNA replication-related pathways and the cell cycle. It was also associated with tumorigenesis and progression of NB.

Conclusion
Cuproptosis, especially PDHA1, play a crucial role in the TIME characteristics, tumor progression, and long-term prognosis of NB. The patterns of cuproptosis assessed in this research may improve the understanding of the overall concept of NB tumorigenesis, thus facilitating the development of more effective therapeutic interventions.
Assessing the effects of 24-epibrassinolide and yeast extract at various levels on cowpea’s morphophysiological and biochemical responses under water deficit stress
Authors: Gholami Faride , Amerian Mohamad Reza , Asghari Hamid Reza , Ebrahimi Amin 
Published: November 2023

Abstract
Background
Due to the factor of water deficit, which has placed human food security at risk by causing a 20% annual reduction in agricultural products, addressing this growing peril necessitates the adoption of inventive strategies aimed at enhancing plant tolerance. One such promising approach is employing elicitors such as 24-epibrassinolide (EBR) and yeast extract, which are potent agents capable of triggering robust defense responses in plants. By employing these elicitors, crops can develop enhanced adaptive mechanisms to combat water deficit and improve their ability to withstand drought condition. This study investigates the impact of different levels of EBR (0, 5, 10 µm) and yeast extract (0 and 12 g/l) on enhancing the tolerance of cowpea to water deficit stress over two growing seasons.

Results
The findings of this study demonstrate that, the combined application of EBR (especially 10 µm) and yeast extract (12 g/l) can increase seed yield (18%), 20-pod weight (16%), the number of pods per plant (18%), total chlorophyll content (90%), and decrease malondialdehyde content (45%) in cowpea, compared to plants grown under water deficit stress without these treatments. Upon implementing these treatments, impressive results were obtained, with the highest recorded values observed for the seed yield (1867.55 kg/ha), 20-pod weight (16.29 g), pods number per plant (9), and total chlorophyll content (19.88 mg g−1 FW). The correlation analysis indicated a significant relationship between the seed yield, and total chlorophyll (0.74**), carotenoids (0.82**), weight of 20 seeds (0.67**), and number of pods (0.90**). These traits should be prioritized in cowpea breeding programs focusing on water deficit stress.

Conclusions
The comprehensive exploration of the effects of EBR and yeast extract across various levels on cowpea plants facing water deficit stress presents a pivotal contribution to the agricultural domain. This research illuminates a promising trajectory for future agricultural practices and users seeking sustainable solutions to enhance crops tolerance. Overall, the implications drawn from this study contribute significantly towards advancing our understanding of plant responses to water deficit stress while providing actionable recommendations for optimizing crop production under challenging environmental conditions.
Geographic distribution and impacts of climate change on the suitable habitats of Rhamnus utilis Decne in China
Authors: Guiquan Song , Jiali Feng , Shuai Gong , Wenya Hao , Xiangkun Kong , Sheng Zhao , Yueling Zhao , Xuelian Jiang 
Published: November 2023

Abstract
Background
Rhamnus utilis Decne (Rhamnaceae) is an ecologically and economically important tree species. The growing market demands and recent anthropogenic impacts to R. utilis forests has negatively impacted its populations severely. However, little is known about the potential distribution of this species and environmental factors that affect habitat suitability for this species. By using 219 occurrence records along with 51 environmental factors, present and future suitable habitats were estimated for R. utilis using Maxent modeling; the important environmental factors affecting its distribution were analyzed.

Results
January water vapor pressure, normalized difference vegetation index, mean diurnal range, and precipitation of the warmest quarter represented the critical factors explaining the environmental requirements of R. utilis. The potential habitat of R. utilis included most provinces from central to southeast China. Under the climate change scenario SSP 245, Maxent predicted a cumulative loss of ca. 0.73 × 105 km2 in suitable habitat for R. utilis during 2041–2060 while an increase of ca. 0.65 × 105 km2 occurred during 2081–2100. Furthermore, under this climate change scenario, the suitable habitat will geographically expand to higher elevations.

Conclusions
The findings of our study provide a foundation for targeted conservation efforts and inform future research on R. utilis. By considering the identified environmental factors and anticipating the potential impacts of climate change, conservation strategies can be developed to preserve and restore suitable habitats for R. utilis. Protecting this species is not only crucial for maintaining biodiversity but also for sustaining the economic benefits associated with its ecological services.
Wheat stripe rust resistance locus YR63 is a hot spot for evolution of defence genes – a pangenome discovery
Authors: Mackenzie Amy , Norman Michael , Gessese Mesfin , Chen Chunhong , Sørensen Chris , Hovmøller Mogens , Ma Lina , Forrest Kerrie , Hickey Lee , Bariana Harbans , Bansal Urmil , Periyannan Sambasivam 
Published: November 2023

Abstract
Background
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a threat to global wheat production. Deployment of widely effective resistance genes underpins management of this ongoing threat. This study focused on the mapping of stripe rust resistance gene YR63 from a Portuguese hexaploid wheat landrace AUS27955 of the Watkins Collection.

Results
YR63 exhibits resistance to a broad spectrum of Pst races from Australia, Africa, Asia, Europe, Middle East and South America. It was mapped to the short arm of chromosome 7B, between two single nucleotide polymorphic (SNP) markers sunCS_YR63 and sunCS_67, positioned at 0.8 and 3.7 Mb, respectively, in the Chinese Spring genome assembly v2.1. We characterised YR63 locus using an integrated approach engaging targeted genotyping-by-sequencing (tGBS), mutagenesis, resistance gene enrichment and sequencing (MutRenSeq), RNA sequencing (RNASeq) and comparative genomic analysis with tetraploid (Zavitan and Svevo) and hexaploid (Chinese Spring) wheat genome references and 10+ hexaploid wheat genomes. YR63 is positioned at a hot spot enriched with multiple nucleotide-binding and leucine rich repeat (NLR) and kinase domain encoding genes, known widely for defence against pests and diseases in plants and animals. Detection of YR63 within these gene clusters is not possible through short-read sequencing due to high homology between members. However, using the sequence of a NLR member we were successful in detecting a closely linked SNP marker for YR63 and validated on a panel of Australian bread wheat, durum and triticale cultivars.

Conclusions
This study highlights YR63 as a valuable source for resistance against Pst in Australia and elsewhere. The closely linked SNP marker will facilitate rapid introgression of YR63 into elite cultivars through marker-assisted selection. The bottleneck of this study reinforces the necessity for a long-read sequencing such as PacBio or Oxford Nanopore based techniques for accurate detection of the underlying resistance gene when it is part of a large gene cluster.
Genome-wide identification and expression patterns of the laccase gene family in response to kiwifruit bacterial canker infection
Authors: Zhang Zhuzhu , Long Youhua , Yin Xianhui , Wang Weizhen , Li Wenzhi , Jiang Lingli , Chen Xuetang , Wang Bince , Ma Jiling 
Published: November 2023

Abstract
Background
Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a destructive disease worldwide. Resistance genes that respond to Psa infection urgently need to be identified for controlling this disease. Laccase is mainly involved in the synthesis of lignin in the plant cell wall and plays a prominent role in plant growth and resistance to pathogen infection. However, the role of laccase in kiwifruit has not been reported, and whether laccase is pivotal in the response to Psa infection remains unclear.

Results
We conducted a bioinformatics analysis to identify 55 laccase genes (AcLAC1–AcLAC55) in the kiwifruit genome. These genes were classified into five cluster groups (I–V) based on phylogenetic analysis, with cluster groups I and II having the highest number of members. Analysis of the exon–intron structure revealed that the number of exons varied from 1 to 8, with an average of 5 introns. Our evolutionary analysis indicated that fragment duplication played a key role in the expansion of kiwifruit laccase genes. Furthermore, evolutionary pressure analysis suggested that AcLAC genes were under purifying selection. We also performed a cis-acting element analysis and found that AcLAC genes contained multiple hormone (337) and stress signal (36) elements in their promoter regions. Additionally, we investigated the expression pattern of laccase genes in kiwifruit stems and leaves infected with Psa. Our findings revealed that laccase gene expression levels in the stems were higher than those in the leaves 5 days after inoculation with Psa. Notably, AcLAC2, AcLAC4, AcLAC17, AcLAC18, AcLAC26, and AcLAC42 showed significantly higher expression levels (p < 0.001) compared to the non-inoculated control (0 d), suggesting their potential role in resisting Psa infection. Moreover, our prediction indicated that 21 kiwifruit laccase genes are regulated by miRNA397, they could potentially act as negative regulators of lignin biosynthesis.

Conclusions
These results are valuable for further analysis of the resistance function and molecular mechanism of laccases in kiwifruit.
Novel beagle model of gastric local fibrotic target lesions for the evaluation and training of endoscopic techniques
Authors: He Xiao-Jian , Wang Xiao-Ling , Jiang Chuan-Shen , Hong Dong-Gui , Lin Hai-Lan , Zheng Yun-Ping , Li Han , Chen Xin-Jiang , Huang Jian-Xiao , Dai Ling-Shuang , Liu Mei-Yan , Luo Bao-Xiang , Li Dong-Liang , Li Da-Zhou , Wang Wen 
Published: November 2023

Abstract
Background
Novel endoscopic techniques used in the treatment of gastric lesions with local submucosal fibrosis need preclinical evaluation and training due to safety limitations. Therefore, the purpose of our study was to establish an animal model of gastric local fibrotic target lesions and assess its feasibility in the evaluation and training of endoscopic techniques.

Methods
In six experimental beagles, a 50% glucose solution was injected into three submucosal areas of the fundus, body, and antrum of the stomach to create gastric local fibrotic target lesions (experimental group). On post-injection day (PID) 7, the injection sites were assessed endoscopically to confirm the presence of submucosal fibrosis formation, and the dental floss clip traction assisted endoscopic submucosal dissection (DFC-ESD) procedure was performed on the gastric local fibrotic target lesions to confirm its feasibility after endoscopic observation. The normal gastric mucosa of six control beagles underwent the same procedure (control group). All the resected specimens were evaluated by histological examination.

Results
All 12 beagles survived without postoperative adverse events. On PID 7, 16 ulcer changes were observed at the injection sites (16/18) under the endoscope, and endoscopic ultrasonography confirmed the local submucosal fibrosis formation in all ulcer lesions. The subsequent DFC-ESD was successfully performed on the 32 gastric target lesions, and the mean submucosal dissection time in the ulcer lesions was greater than that in the normal gastric mucosa (15.3 ± 5.6 vs. 6.8 ± 0.8 min; P < 0.001). There was no difference in rates of en bloc resection, severe hemorrhage, or perforation between the two groups. Histological analysis of the ulcer lesions showed the absence of epithelial or muscularis mucosae and extensive submucosal fibrous tissue proliferations compared with normal gastric mucosa. Overall, endoscopists had high satisfaction with the realism and feasibility of the animal model.

Conclusion
We developed a novel animal model of gastric local fibrotic target lesions to simulate difficult clinical situations, which strongly appeared to be suitable for the preclinical evaluation and learning of advanced endoscopic techniques.
Older adults in the digital health era: insights on the digital health related knowledge, habits and attitudes of the 65 year and older population
Authors: Győrffy Zsuzsa , Boros Julianna , Döbrössy Bence , Girasek Edmond 
Published: November 2023

AbstractBackgroundThe COVID-19 pandemic has increased internet use by older age groups to an unprecedented level in Hungary mirroring the general tendency in the total population. Nevertheless, international trends indicate that this group is less likely to use digital health technologies than younger ones. The aging population raises the question of successfully integrating elderly people into the digital health ecosystem. Our research aim is to investigate the digital health usage patterns and attitudes of the population aged 65 and over through a representative sample.MethodsA national representative questionnaire survey was conducted by telephone (CATI), interviewing 1723 respondents. Within this sample we examined 428 people in the over-65 age group, 246 in the 65–74 age group and 182 in the over-75 age group. Predictors of demand for digital solutions were tested using binary logistic regression model.Results50.8% of people aged 65–74 and 37.1. % of people aged 75 + use the internet for health-related purposes, mostly to access websites. 85% of respondents in 65–74 and 74% in 75 + age group have used more than one digital health device and around 70% of both age groups have a need for more than one digital solution. 90.2% (64–75 age group) and 85.7% (75 + age group) of respondents are familiar with e-prescription, 86.4% and 81.4% of them use it. 77.1% of 65–74-year-olds have heard of and nearly half 45.5% have used online appointment. More than half (52.7%) of the respondents in this age group have heard of and used electronic transmission of medical records and data. A similar proportion has heard about and used apps: 54.3% has heard of them, but only 17.3% has used them. The multivariate analyses emphasized that the need for digital solutions increases with the level of education and the more benefits one perceives in using digital solutions.ConclusionOur research has shown that the senior age group has measurable needs in the field of digital health, so helping them on this journey is in the interest of the whole health ecosystem. Their high level of interest is indicated by the fact that more than a fifth of older adults would like to have access to between 7 and 10 of the maximum number of digital devices available. The differences between the two age groups - with younger people being more open to digital solutions and using them more - and the fact that the under 65s are better adapted digitally in all respects, raises the possibility that the specific trends in digital health for older people may virtually disappear in 10 years’ time (when the under 65s now enter this age group).