bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the free-radical polymerization of vinyl monomers using horseradish peroxidase (HRP) nanoflowers
Authors: Ozaydin Gulbahar , Mirioglu Muge , Dadi Seyma , Ocsoy Ismail , Gokturk Ersen 
Published: January 2025

Abstract
In this study, we report the production of flower-shaped HRP-Cu2+ hybrid nano biocatalyst (HRP-Cu2+ HNF) from the complexation between horseradish peroxidase (HRP) enzyme and Cu2+ ions, and investigate catalytic activity and stability of the obtained nanoflowers on the polymerization of some vinyl monomers (styrene, methylmethacrylate, acrylamide and N-isopropylacrylamide). Polymerizations of these monomers, except water soluble acrylamide, were accomplished under emulsion conditions using cationic, anionic and non-ionic surfactants in the presence of hydrogen peroxide (H2O2) and 2,4-pentanedione mediator. Optimum polymerizations were achieved under the conditions of non-ionic surfactant (tween 40) used. HRP-Cu2+ HNF mediated polymerizations resulted in very high yields and molecular weights (M
n) of the polymers. Optimum polymerization of styrene with 84% of yield (M
n = 319 kDa) was accomplished at room temperature. However, the highest polymerization yields for acrylamide (96%, M
n = 171 kDa) and N-isopropylacrylamide (85%, M
n = 185 kDa) was achieved at 70 °C. Similarly, optimum polymerization of methylmethacrylate was accomplished with 84% of yield (M
n = 190 kDa) at 60 °C. While free-HRP loses its catalytic activity at 60 °C and above temperatures, HRP-Cu2+ HNF showed very high catalytic activity and stability even at 70 °C. Increasing activity and stability of hybrid nanoflowers provide significant advantages for both scientific and industrial applications.
The prognostic value of baseline EARL standardized FDG PET indices in pediatric and adolescent high-grade osteosarcoma
Authors: van Ewijk Roelof , Dandis Rana , Rodewijk Janna , de Keizer Bart , ter Horst Simone A. J. , van de Sande Michiel A. J. , van der Heijden Lizz , Merks Johannes H. M. , Haveman Lianne M. , Braat Arthur J. A. T. 
Published: January 2025

Abstract

Objective
To investigate the prognostic value of baseline European Association of Nuclear Medicine Research Ltd. (EARL) standardized [18F]fluorodeoxyglucose positron emission tomography-computed tomography ([18F]FDG PET-CT) quantitative values for survival and to evaluate cutoff values identified in other studies.

Materials and methods
Pediatric and adolescent patients with high-grade osteosarcoma were included. Baseline [18F]FDG PET-CT, with EARL-accredited reconstructions, was the standard diagnostic staging procedure. Cox proportional hazard analysis for event-free survival (EFS) and overall survival (OS) was performed with clinical prognostic factors. Kaplan–Meier analysis and log-rank tests were applied to investigate the prognostic performance of the [18F]FDG PET indices.

Results
In total, 66 patients were included in this study. In the univariable Cox regression analysis, peak lean-body mass corrected SUV (SULpeak) (hazard ratio (HR): 1.04), total lesion glycolysis (TLG) (HR: 1.0), and metabolic tumor volume (MTV) (HR: 1.0) were not associated with EFS or OS. Log-rank analysis showed a significant difference in EFS for all SULmax and SULpeak cutoffs. For MTVtotal the maximum Youden, and for TLGtotal, the maximum Youden and maximally selected rank cutoff resulted in a significant EFS difference. No cutoff for any measure showed a significant difference in OS between the groups. ROC curves for event status had an AUC of 0.67, 0.66, 0.64, and 0.64 for SULmax, SULpeak, MTVtotal, and TLGtotal, respectively.

Conclusion
In this study, the baseline EARL-standardized [18F]FDG PET indices of children and adolescents with osteosarcoma were not prognostic of EFS or OS. The proposed prognostic cutoffs from earlier studies suffer from important technical and statistical issues.

Key Points

Question

Prognostic value of baseline [

18

F]FDG PET-CT imaging markers have been reported for histologic response and survival in high-grade osteosarcoma but have not been validated for clinical practice.

Findings

Baseline SUV

peak
, TLG

total

, and MTV

total

measured on EARL-accredited reconstructions were not prognostic factors for survival in pediatric and adolescent patients with high-grade osteosarcoma.

Clinical relevance

A wide range of values for SUV

peak

and SUV

max

cutoffs with similar prognostic value were identified, questioning the value of a single proposed cutoff. Lack of validation, with important technical and statistical issues of proposed prognostic cutoffs, limits clinical implementation.
Liming enhances the abundance and stability of nitrogen-cycling microbes: the buffering effect of long-term lime application
Authors: Mitsuta Akari , Lourenço Késia Silva , Chang Jingjing , Ros Mart , Schils René , Uchida Yoshitaka , Kuramae Eiko Eurya 
Published: January 2025

Abstract
Lime application (liming) has historically been used to ameliorate soil acidity in grasslands. Liming effectively improves soil pH, plant productivity, and soil physicochemical properties, but the long-term impact of acidity control by liming on key microbial nitrogen (N)-cycling genes in semi-natural grasslands is unknown. We investigated the effect of 65 years of liming on N-cycling processes in the limed and control plots of the Ossekampen long-term grassland experiment in the Netherlands. These plots have not received any other fertilizers for 65 years. Soil sampling and nitrous oxide (N2O) emission measurements were conducted three times in spring and four times in summer, and quantitative real-time PCR was performed to determine the absolute abundances of N-cycling genes, including ammonia-oxidation (amoA-AOB, amoA-AOA, amoA-comammox), denitrification (nirS, nirK, nosZ), nitrate ammonification (nrfA), and N-fixation (nifH) genes. Long-term liming increased the absolute abundances of nitrifiers, denitrifiers, and nitrate ammonifiers. Soil N2O emissions did not differ significantly between liming and control treatments. Additionally, liming had a buffering effect that maintained the population of N-cycling microbes against seasonal variations in abundance. Our results indicate that improving soil acidity through liming potentially facilitates microbial N-cycling processes without increasing N2O emissions.
Das Grubenrettungsseminar in der Ausbildung der Montanuniversität Leoben
Authors: Sifferlinger Nikolaus August , Schön Wilhelm , Knoll Birgit 
Published: January 2025

Zusammenfassung
Die Absolventen der Studienrichtung Rohstoffingenieurswesen werden, wenn sie in Untertagebetrieben zu arbeiten beginnen, rasch in unterstützender, aktiver oder leitender Funktion mit der Grubenwehr in Berührung kommen. Um sie darauf vorzubereiten, wird an der Montanuniversität Leoben das Grubenrettungsseminar angeboten.
Im Grubenrettungsseminar lernen sie die Grundlagen für die Arbeit der Grubenwehr und der betrieblichen Notfallplanung kennen. Durch die Teilnahme als Beobachter an einer Grubenwehrübung werden auch Einblicke in die praktische Rettungsarbeit ermöglicht. Damit sind die Absolventen auch auf das betriebliche Rettungswesen im Bergbau gut vorbereitet.
Die Montanuniversität Leoben bildet die Absolventen der Studienrichtung Rohstoffingenieurswesen im Grubenrettungswesen aus und hält sich durch nationale und internationale Zusammenarbeit am neuesten Stand der Technik und Organisation.
A new 3D full-body scanner analyzing the sagittal and coronal balance of the adult spine: a preliminary prospective observational study
Authors: Kang Tae Hoon , Jang Seokin , Seo Inwook , Choi Minseok , Park Yongsoo , Lee Yohan , Lee Jae Hyup , Cho Minjoon 
Published: January 2025

Abstract

Background
The degenerative spondylosis can cause the difficulty in maintaining sagittal and coronal alignment of spine, and X-ray parameters are the gold standard to analyze the malalignment. This study aimed to develop a new 3D full body scanner to analyze the spinal balance and compare it to X-ray parameters.

Methods
Ninety-seven adult participants who suffer degenerative spondylosis underwent 3D full body scanning, whole spine X-rays, clinical questionnaires and body composition analyses. The 5 inflection points (ear, shoulder, hip, knee, ankle) of the 3D scanner in the sagittal plane were automatically labeled by an AI algorithm. Three concepts are created including “the angle between two points with respect to the plumb line”, “the horizontal distances between two points in the sagittal plane” and “the angle between three points”. For the coronal plane, the shoulder gradient was analyzed. X-ray parameters of cervical, thoracolumbar and whole spine sagittal balance and coronal balances were compared. The body composition data and clinical questionnaire scores were compared to x-ray and 3D scanner parameters.

Results
The correlation coefficient (C.C.) of dAB_hor (horizontal distance between ear and shoulder in the sagittal plane) and C2-C7 SVA was 0.478 (p-value < 0.001). The C.C. of aAC_sag (sagittal angle of ear-hip from the plumb line) and ODHA was 0.336 (p < 0.001). About coronal balance, the C.C. of shoulder gradient and clavicle angle from x-ray was 0.373 (p < 0.001). The C.C.s were merely affected by body composition data. But in multiple regression analysis, BMI affected 3D scanner data. Clinical symptoms showed correlations with aBCD(shoulder-hip-knee) and aCDE(hip-knee-ankle angle), which may reflect a compensatory pelvic retroversion and knee flexion for positive sagittal imbalance.

Conclusions
This new 3D scanner has some strengths like radiation-free methods, correlation with x-ray parameters and clinical symptoms, independence to body composition data, and possibility of analyzing dynamic spine balance.
Radiological and anatomical evaluation of the internal venous system in the context of access to the third ventricle - proposal of a new classification
Authors: Zaczkowski Karol , Łabętowicz Piotr , Podstawka Małgorzata , Wójcik Rafał , Bobeff Ernest Jan , Zielińska Nicole , Szmyd Bartosz , Ramirez Manuel de Jesus Encarnacion , Ramirez Issael , Olewnik Łukasz , Jaskólski Dariusz Jan , Wiśniewski Karol 
Published: January 2025

Abstract

Background
The internal venous system of the brain is a crucial anatomical landmark during accesses to the third ventricle through the foramen of Monro. Many classifications based on radiological assessment of the system have been developed, but they tend to be descriptive and do not highlight favorable anatomical variants. The aim of our study was to create a system based on morphometric measurements to facilitate preoperative decision-making regarding access to third ventricle tumors.

Methods
We conducted an analysis of 119 MRI scans with SWI sequence using BrainLab software to create a model of the ventricular system, which allowed us to perform radiological measurements. We then validated these findings anatomically using 32 human brain specimens. The analyzed structures included the foramen of Monro (FM), the anterior septal vein (ASV), the thalamostriate vein (TSV), the venous angle (VA), the internal cerebral vein (ICV), and the distance between the FM and VA.

Results
Based on the radiological analysis, we identified 9 internal venous systems, accounting for variations in each analyzed structure. The statistical analysis revealed no differences in the frequency of subtypes between radiological and anatomical studies (p = 0.097), nor in the occurrence of false venous angles (p = 0.520). We identified venous configurations that, in our assessment, are unfavorable in the context of accessing the third ventricle.

Conclusion
The resulting classification accounts for significant clinical anatomical variations and, for the first time, provides specific morphometric values for each anatomical subtype. Consequently, it serves as a reproducible reference framework for preoperative planning of access to the third ventricle.
Coronectomy of mandibular third molars with dental pathology: a prospective cohort study of 121 molars
Authors: van Bodegraven Arjan , Simons Rashida N. , Tuk Jacco G. , de Lange Jan , Lindeboom Jerome A. H. 
Published: January 2025

Abstract

Purpose
Coronectomy is a valuable treatment proven safe for non-pathological mandibular third molars with an increased risk of inferior alveolar nerve injury. Coronectomy may also be useful for mandibular third molars with dentigerous cysts and caries, but this is not commonly performed due to the lack of well-designed, evidence-based studies. Here, we aim to investigate the safety of coronectomy for mandibular third molars with caries and dentigerous cysts.

Methods
One hundred fifteen patients with an impacted mandibular third molar with a dentigerous cyst or caries underwent coronectomy or complete removal and received follow-up with clinical and radiographical examinations. We statistically compared the presence of postoperative complications after coronectomy versus complete removal.

Results
Data from 121 molars were available for analysis. The results revealed no significant difference in the occurrence of postoperative complications (including persistent pain, inferior alveolar nerve injury, infection, alveolar osteitis, excessive bleeding, and the need for retreatment) between coronectomy and complete removal. Additionally, the incidence of postoperative complications was not related to any analyzed patient- or molar-related factors, including age, gender, health status, smoking, caries, dentigerous cyst extent, and impaction degree.

Conclusion
For pathological impacted mandibular third molars with dentigerous cysts and caries, coronectomy exhibited both short-term and long-term safety, showing no significant difference in postoperative complications compared to total removal. Our results suggest that coronectomy can be indicated for pathological mandibular third molars with proximity to the inferior alveolar nerve.

Trial registration number
Not applicable.
ML-based tooth shade assessment to prevent metamerism in different clinic lights
Authors: Karcioglu Abdullah Ammar , Efitli Esra , Simsek Emrah , Ozdogan Alper , Karatas Furkan , Senocak Tuba 
Published: January 2025

Abstract
The aesthetic understanding has found its place in dental clinics and prosthetic dental treatment. Determining the appropriate prosthetic tooth color between the clinician, patient and technician is a difficult process due to metamerism. Metamerism, known as the different perception of the color of an object under different light sources, is caused by the lighting differences between the laboratory and the dental clinic. The traditional trial-error color determination method, coupled with the high cost of instrumental color value determination, has prompted the need for alternative technologies. The integration of AI technologies into dental practices aims to minimize errors in tooth shade assessment, reduce equipment usage, eliminate the impact of clinic lighting on color detection, and decrease costs for patients, dentists, and laboratories. In this study, a machine learning (ML) based approach that can correctly detect tooth shade even under different clinical lights has been developed. A dataset consisting of 580 dental images taken under four different clinical lights and with five repetitions was created using the Vita color shade guide. Experimental studies were performed using the HSV color space, 6 different ML algorithms and color histograms. As a result, 97.93% accuracy rate was achieved by using cross-validation (cv = 5) in the classification of 29 color values ​​independent of clinical lights. It has been shown that the tooth colors can be determined with high accuracy using ML algorithms and metamerism can be prevented.
First-time unprovoked seizures in adults: when and how to treat
Authors: Catania Cecilia , Bernasconi-Xhepa Sophie , Seeck Margitta 
Published: January 2025

Abstract
First-time unprovoked seizures are relatively common events in the general population. However, they may carry an elevated risk of recurrence, which can lead to the diagnosis of epilepsy. Identifying risk factors for recurrence and assessing the likelihood of relapse are key elements in the initial management of these events to determine whether antiseizure medication (ASM) should be introduced. This paper discusses possible treatments after a first-time epileptic seizure in the context of new-onset epilepsy. The importance of early and comprehensive work-up is highlighted given the poorer prognosis, at least during the first 2 years, if other seizures occur. We also discuss the choice of ASM monotherapy based on seizure type and individual clinical factors.
Population genomics and genetic diversity of the invasive chrysanthemum lace bug (Corythucha marmorata) across its invasive range in Japan
Authors: Contreras-Negrete Gonzalo , Meza-Lázaro Rubi N. , Benites Pilar , Becerra-Lira Víctor E. , Sakata Yuzu , Ohgushi Takayuki , Craig Timothy P. , Itami Johanne , Zaldívar-Riverón Alejandro , Hernández-López Antonio 
Published: January 2025

Abstract
The escalating global movement of alien species, facilitated by increased trade and travel, poses a pressing need to comprehend their invasive potential and the consequent ecological and economic ramifications. Despite a growing body of evidence on rapid evolutionary shifts in invasive species, comprehensive insights into the genetic variability underlying these adaptations are constrained by limited genomic resources. Understanding the role of genetic variation in the success or failure of biological invaders is thus crucial. This study focuses on the chrysanthemum lace bug, Corythucha marmorata, as a model system to investigate the interplay of genetic variation and invasion dynamics. Our analysis reveals moderate genetic structure among countries, with significant genetic differentiation observed within populations. Mitochondrial COI DNA and haplotype network analysis revealed shared haplotypes between Japan and North America, indicating recent events of introduction, while exclusive Japanese haplotypes and significant FST and GST values suggest local divergence. Phylogenetic and STRUCTURE analyses show genetic clusters unique to Japan, with populations like SAG and CER displaying higher divergence. Bottlenecks followed by divergence, as indicated by the DIYABC-RF analysis, point to a complex evolutionary history involving multiple introductions and subsequent local divergence in Japan.
Anxiety Symptoms Predict Subsequent Depressive Symptoms in Neurodivergent Youth: A 10-Year Longitudinal Study
Authors: Orm Stian , Wood Jeffrey J. , Fossum Ingrid Nesdal , Adams Keenan , Andersen Per Normann , Fjermestad Krister , Øie Merete Glenne , Skogli Erik Winther 
Published: January 2025

Abstract
Neurodivergent youth often experience anxiety and depressive symptoms that may hamper adaptive functioning and well-being. There is little knowledge of how anxiety and depression are related in neurodivergent youth. Therefore, we aimed to examine whether the relationship between anxiety and depressive symptoms is uni- or bidirectional in neurodiverse youth. We assessed self-reported anxiety and depressive symptoms over time in 173 youth (M

baseline age
= 11.7 years, SD = 2.1, 64% males, 36% females). The sample comprised 38 autistic youth, 85 youth diagnosed with attention-deficit/hyperactivity disorder (ADHD), and 50 comparison youth assessed at baseline (T1), 2-year follow-up (T2, 97% retention), and 10-year follow-up (T3, 73% retention). We used cross-lagged models to analyze the data. In neurodivergent youth, more anxiety symptoms at T1 and T2 predicted more depressive symptoms at T2 and T3. Preceding anxiety symptoms were linked with later depressive symptoms, even after accounting for autoregressive effects of depressive symptoms. The results are consistent with a prodromal model in which anxiety symptoms can independently foreshadow the emergence of depressive symptoms over the course of development among neurodivergent youth. Potentially, addressing anxiety symptoms among youth with autism or ADHD could play a role in preventing the onset of youth depression.
“DNA Doesn’t Lie:” Genetic Essentialism and Determinism in Law & Order: Special Victims Unit
Authors: Lillydahl Alice , Clayton Jay 
Published: January 2025

Abstract

Law and Order: Special Victims Unit (SVU) (1999–present) is a popular primetime drama that spotlights the use of genetic information to solve crimes. Despite the show’s heavy reliance on the forensic use of DNA evidence, the role of genetics in defining family and identity arises in complex ways. Many episodes wrestle with social, ethical, and legal questions that reflect assumptions about genetic essentialism and genetic determinism, but counterarguments about the importance of non-biological relationships, social factors, and legal entitlements are given equal or greater weight. For this study, we identified and viewed 38 episodes from SVU’s first twenty seasons centered on genetic themes in non-forensic contexts. Two recurring themes emerged: (1) that the role DNA plays is only one factor in a complex web of biological and social considerations that shape our understanding of kinship; and (2) that genetic predispositions to behavioral traits such as mental illness or violence should not be seen as obscuring the responsibility of personal choice. By treating genetics as a complex source of information requiring social context to be understood, SVU allows audiences to play an active role in interpreting its meaning.
Rupture zones of the 1978 (Mw 7.6) and 2020 (Mw 7.4) earthquakes in the Oaxaca subduction zone: Implications for seismic slip and seismic hazard
Authors: Martínez-López M. Rosario , Suárez Gerardo , Mendoza Carlos 
Published: January 2025

Abstract
The slip models of the Oaxaca, Mexico earthquakes of 29 November 1978 (M

w
7.6) and 23 June 2020 (Mw 7.4) were estimated by inverting P and SH teleseismic velocity waveforms. The inversion of the 1978 event used broadband and long-period data. In the case of the 2020 event, broadband data were available. In both cases, the rupture zones lie down dip of the hypocenter. It has been suggested that the events of 1978 and 2020 are quasi-repeater earthquakes breaking similar asperities of previous events. Based on this, the slip of the seismic rupture obtained in recent events is used to characterize the slip of previous events, and to calculate the slip deficiency in the four rupture zones defined by the 1928 events. The largest slip deficiency is where the large 7.6 event occurred in October 1928, between the ruptures of 1978 and the Mw 7.2 earthquake of June 2018. Here, no great earthquakes have occurred in the last 96 years, suggesting high accumulation of elastic strain that may generate potentially an earthquake Mw 7.8. This gap separates two regions with different seismic behavior, suggesting a complex rupture process in the Oaxaca subduction zone. The other three regions, where the 1978, 2018, and 2020 earthquakes took place, show average slip deficiencies of 500 cm. The great earthquake of 1787 broke the four rupture areas defined by the 1928 events in a single Mw 8.6 earthquake, consistent with a variable rupture mode that has been observed in other subduction zones of the world. In conclusion, the Oaxaca subduction zone suggests a high seismic potential.