bluuebunny/crossref_metadata_2025_split huggingface dataset:
The m6A reader IGF2BP2 regulates glycolytic metabolism and mediates histone lactylation to enhance hepatic stellate cell activation and liver fibrosis
Authors: Li Xun , Zhou Yongqiang , Yan Jiexi , Huang He , Liu Lu , Ren Longfei , Hu Jinjing , Jiang Xiaoxu , Zheng Yan , Xu Lingcong , Zhong Fupeng 
Published: November 2023

Abstract
Evidence for the involvement of N6-Methyladenosine (m6A) modification in the etiology and progression of liver fibrosis has emerged and holds promise as a therapeutic target. Insulin-like growth factor 2 (IGF2) mRNA-binding protein 2 (IGF2BP2) is a newly identified m6A-binding protein that functions to enhance mRNA stability and translation. However, its role as an m6A-binding protein in liver fibrosis remains elusive. Here, we observed that IGF2BP2 is highly expressed in liver fibrosis and activated hepatic stellate cells (HSCs), and inhibition of IGF2BP2 protects against HSCs activation and liver fibrogenesis. Mechanistically, as an m6A-binding protein, IGF2BP2 regulates the expression of Aldolase A (ALDOA), a key target in the glycolytic metabolic pathway, which in turn regulates HSCs activation. Furthermore, we observed that active glycolytic metabolism in activated HSCs generates large amounts of lactate as a substrate for histone lactylation. Importantly, histone lactylation transforms the activation phenotype of HSCs. In conclusion, our findings reveal the essential role of IGF2BP2 in liver fibrosis by regulating glycolytic metabolism and highlight the potential of targeting IGF2BP2 as a therapeutic for liver fibrosis.
Emotion perception in Behavior frontotemporal dementia and Alzheimer’s disease: the parahippocampal conundrum
Authors: Morais Ricardo Félix , Duro Diana , Pires Ricardo , Lemos Raquel , Lima Marisa , Oliveira Tiago Gil , Santana Isabel 
Published: November 2023

Abstract
Introduction
Emotional perception has been described as the capacity which enables humans to correctly identify cues that guide interpersonal interaction, forming the basis for social behavior. The parahippocampal cortex seems to be a key player in mediating the association between context and emotion, thus helping perceiving emotions in others. Behavioral Variant Frontotemporal Dementia (bvFTD) and Alzheimer’s Disease (AD) patients appear to exhibit deficits in this process. Studying how emotion perception is impaired in these patients may provide insights into social cognition mechanisms, emphasizing the parahippocampal role in social behavior.
Methods
We have included convenience samples of 29 bvFTD, 25 AD and 15 controls. Extensive[IS1] [RM2] neuropsychological assessment was performed, complemented with the Comprehensive Affect Testing System (CATS) thus enabling a nuanced evaluation of emotion perception process. Multiple regression analysis was used to assess the relation between CATS main quotients scores and parahippocampal thickness (p £ 0,001).
Results
BvFTD patients obtained higher scores in cognitive tests than AD but had a more severe deficit in social behavior. Interestingly, the bvFTD group revealed a significant deficit in all three emotional quotients (face, prosody and global) evaluated by the CATS battery, with a statistically significant relation with parahippocampal thickness.
AD group displayed milder deficits in all three emotion quotients, although facial emotion recognition deficits were close to reaching statistical significance.
Conclusion
Our data suggest a profound emotion perception deficit in bvFTD, supporting the pivotal role of the parahippocampal gyrus in context associations. Targeted rehabilitation with the objective of reducing these emotion deficits might be valuable.
Effect of organic carbon sources on the growth of Chaetoceros muelleri
Authors: Li Keyi , Xia Yutong , Wang Zhen , Chen Yanzhen , Feng Yong , Gao Ebin , Huo Shuhao , Chen Huayou 
Published: November 2023

Abstract
Bait microalgae are essential for the successful cultivation of shellfish on a large scale. However, during periods of low light such as winter or rainy days, the production of microalgae is often limited due to the unavailability of affordable, reliable, and efficient carbon sources. Therefore, in this study, Chaetoceros muelleri, a favorite of shellfish, was chosen as the experimental algal strain for this investigation, and different concentrations of organic carbon sources were added to the F/2 medium for pure cultivation experiments of Chaetoceros muelleri. When 0.5 g L− 1 acetic acid, 0.5 g L− 1 citric acid, and 0.3 g L− 1 succinic acid were added, the algal cell density of Chaetoceros muelleri was 2.10, 2.07, and 1.67 times greater than that of the control group, according to the single-factor experiment's findings. The response surface optimization study showed that when acetic acid 0.599 g L− 1, citric acid 0.492 g L− 1, and succinic acid 0.208 g/L, the algal cell density was 1.41×107 CFU mL− 1, which was 1.15 times that of single acetic acid culture (1.23×107 CFU mL− 1) and 3.28 times that of single glucose culture (0.43×107 CFU mL− 1). Thus, acetic acid is more effective in promoting the growth of Chaetoceros muelleri compared to glucose or other organic acids. Furthermore, a combination of acetic acid and other organic acids proves to be even more advantageous. This finding is significant in the quest for suitable organic carbon sources to increase Chaetoceros muelleri production.
A novel homozygous germline mutation in transferrin receptor 1 (TfR1) leads to combined immunodeficiency and provides new insights into iron-immunity axis
Authors: Aba Ümran , Maslak Ibrahim Cemal , Ipşir Canberk , Pehlivan Damla , Warnock Nicholas , Tunes Damon , Cildir Gökhan , Erman Baran 
Published: November 2023

Abstract
A homozygous missense mutation in the transferrin receptor 1 (TfR1), also known as CD71, leads to a rare inborn error of immunity (IEI) characterized by the impaired lymphocyte activation and proliferation due to defective iron uptake of cells. However, only one causative mutation (c.58T>C, p.Y20H) in the TFRC gene coding for TfR1 has been reported so far. We herein identified a new disease-causing homozygous germline mutation in the TFRC gene (c.64C>T, p.R22W) (referred to as TfR1R22W from now on) in a Turkish patient with combined immunodeficiency (CID). TfR1R22W results in impaired TfR1 internalization similar to previously defined TfR1Y20H mutation. We found that TfR1R22W is associated with severely restricted B and T lymphocyte clonal diversity, impaired T cell activation and cytokine production as well as defective mitochondrial oxidative phosphorylation in helper T cells. In addition, circulating NK, Treg and MAIT cell populations were significantly decreased in the patient. Using whole transcriptome analysis, we found dysregulated immune homeostasis and novel biological processes associated with TfR1R22W. We also identified a considerable expansion of circulating low-density neutrophils (LDNs) in patient’s PBMCs. Overall, TfR1R22W mutation expands the current understanding of the IEI associated with TfR1 dysfunction and provides new insights underlying impaired immune function, lymphocyte diversity and granulocyte homeostasis.
Traditional Chinese medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation
Authors: Sun Yang , Zhang Chenyang , Cao Xinran , Zhao Lixin , Du Haojie , Qu Jiao , Zhu Jianxia , Sun Haiyan , Ouyang Zijun 
Published: November 2023

Abstract
Background Psoriasis is a chronic inflammatory genetic disease, mainly manifesting in the skin. Conventional therapies, such as glucocorticosteroids and corticosteroids, have adverse effects that limit drug use. Hence, it is imperative to identify a new therapeutic strategy that exhibits a favorable safety profile. Shi-Bi-Man (SBM) is a safe herbal supplement sourced from various natural plants, including ginseng, angelica sinensis, polygonum multiflorum, and aloe vera.
Purpose We aimed to find a potential treatment for psoriasis and investigate the underlying mechanism through which SBM alleviates psoriatic-like skin inflammation in mice.
Methods We investigated the effects of supplementing with SBM through intragastric administration or smear administration in a murine model of imiquimod-induced psoriasis. The changes in body weight and Psoriasis Area and Severity Index (PASI) score were recorded throughout the entire process. Additionally, we utilized hematoxylin-eosin staining to observe the skin structure and performed single-cell RNA sequencing to explore the underlying mechanism of SBM in influencing the psoriasislike phenotype. Immunofluorescence was conducted to verify our findings. Furthermore, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was employed to investigate the impact of TSG on the expression levels of IL23 in HaCaT cells.
Results SBM remarkably alleviated the psoriasis-like phenotype by inhibiting IL-23/Th17 cell axis. Single-cell RNA sequencing analysis revealed a decrease in the expression of Il17 and Il23 in keratinocytes and T cells, concomitant with a reduction in the proportion of Th17 cells. Meanwhile, the activation of endothelial cells was inhibited, accompanied by a decrease in the expression of Cxcl16. In vitro, the addition of TSG to HaCaT cells resulted in significant suppression of IL23 expression stimulated by TNF-α.
Acidification induce chemcial and microbial variation in tea plantation soils and bacterial degradion of the key acidifying phenolic acids
Authors: Zhang Yuhan , Wang Binjie , Wang Guiwei , Zheng Zhisheng , Chen Ying , Li Ou , Peng Yulong , Hu Xiufang 
Published: November 2023

Abstract
Camellia sinensis is an important economic plant grown in southern subtropical hilly areas, especially in China, mainly for the production of tea. Soil acidification is a significant cause of the reduction of yield and quality and continuous cropping obstacles in tea plants. Therefore, chemical and microbial properties of tea growing soils were investigated and phenolic acid-degrading bacteria were isolated from a tea plantation. Chemical and ICP-AES investigations showed that the soils tested were acidic, with pH values of 4.05–5.08, and the pH negatively correlated with K (p < 0.01), Al (p < 0.05), Fe and P. Aluminum was the highest (47–584 mg/kg) nonessential element. Based on high-throughput sequencing, a total of 34 phyla and 583 genera were identified in tea plantation soils. Proteobacteria and Acidobacteria were the main dominant phyla and the highest abundance of Acidobacteria was found in three soils, with nearly 22% for the genus Gp2. Based on the functional abundance values, general function predicts the highest abundance, while the abundance of amino acids and carbon transport and metabolism were higher in soils with pH less than 5. According to Biolog Eco Plate™ assay, the soil microorganisms utilized amino acids well, followed by polymers and phenolic acids. Three strains with good phenolic acid degradation rates were obtained, and they were identified as Bacillus thuringiensis B1, Bacillus amyloliquefaciens B2 and Bacillus subtilis B3, respectively. The three strains significantly relieved the inhibition of peanut germination and growth by ferulic acid, p-coumaric acid, p-hydroxybenzoic acid, cinnamic acid and mixed acids. Combination of the three isolates showed reduced relief of the four phenolic acids due to the antagonist of B2 against B1 and B3. The three phenolic acid degradation strains isolated from acidic soils display potential in improving the acidification and imbalance in soils of C. sinensis.
Investigation of laser doping on the formation of selective emitter solar cells
Authors: Meziani Samir , Moussi Abderrahmane , Chaouchi Sofiane , Guendouzi Awatif 
Published: November 2023

Abstract
We investigated laser parameters for a laser doping (LD) process that enables to improve cell characteristics through the formation of a selective emitter (SE) multicrystalline silicon solar cell. In this work, the aim is the formation of SE with an investigation of the effect of critical LD parameters, such as laser power and laser speed. The LD 532 nm is used to obtain highly doped regions and deep doping depth that will receive the screen printed silver grid contact. The optimized laser power and speed of 80%, 500 mm/s, induced the activation of the phosphorus dopant and its diffusion in the silicon then leads to a local decrease of the emitter sheet resistance from 60 Ω/sqr to 30 Ω/sqr. Moreover, it was determined by SIMS and ECV measurements that the dopant concentration increased and the doping depth became deeper in the selective emitter formed by increasing laser power and/or laser speed. Fortunately, the surface cracks damage were not observed. The only damage caused by laser irradiation evidenced by debris and a melted surface. We have successfully developed SE p-type mc-Si 4 inch wafer produced by CRTSE solar cell with FF significantly improved of 1.01%. The conversion efficiency of solar cells with selective emitter formed by LD was increased. This gain is due to improved short-circuit current density and open-circuit voltage.
Exploration of quality variation and stability of hybrid rice under multi-environments
Authors: Chen Rirong , Li Dongxu , Fu Jun , Fu Chenjian , Qin Peng , Zhang Xuanwen , Sun Zhenbiao , He Kui , Li Liang , Zhou Wei , Wang Yingjie , Wang Kai , Liu Xuanming , Yang Yuanzhu 
Published: November 2023

Abstract
Improving quality is an essential goal of rice breeding and production. However, rice quality is not solely determined by genotype, but is also influenced by the environment. Phenotype plasticity refers to the ability of a given genotype to produce different phenotypes under different environmental conditions, which can be a representation of the stability of traits. Seven quality traits of 141 hybrid combinations, deriving from the test-crossing of 7 thermosensitive genic male sterile (TGMS) and 25 restorer lines, were evaluated at 5 trial sites with an intermittent sowing of three to five in the Southern China. In the Yangtze River Basin, it was observed that delaying the sowing time of hybrid rice combinations leads to an improvement in their overall quality. Twelve parents were identified to have lower plasticity general combing ability (GCA) values with increased ability to produce hybrids with a more stable quality. The parents with superior quality tend to exhibit lower GCA values for plasticity. The genome-wide association study (GWAS) identified 13 and 15 quantitative trait loci (QTLs) associated with phenotype plasticity and BLUP measurement, respectively. Additionally, there was an overlap of 7 QTLs that simultaneously affecting both terms. Two cloned rice quality genes, ALK and GL7, may be involved in controlling the plasticity of quality traits in hybrid rice. The direction of the genetic effect of the QTL6 (ALK) allele on alkali spreading value (ASV) plasticity varies in different cropping environments. This study provides novel insights into the dynamic genetic basis of quality traits in response to different cropping regions, cultivation practices and changing climate. These finding establish a foundation for precise breeding and production of stable and high-quality rice.
Power Regulation of V2G System for Grid Power Quality Improvement
Authors: Qu Yanqing , Zhao Shuang 
Published: November 2023

Abstract
Electric vehicles (EVs) are likely to have a continuing presence in the vehicle market all over the world for the next few decades. When a large number of EVs are charged simultaneously, they could make an extra burden on the distribution grid, whereas their energy storage units possess a great potential to support the grid through the regulation of both active and reactive power. Vehicle to grid (V2G) is being introduced recently as one of the new technologies for such a purpose. This paper presents the grid support from the vehicles for power quality improvement based on V2G systems, in particular the possibility to regulate the active and reactive power to the power grid when there is voltage and frequency fluctuation caused by load variation. A four quadrant operation for converter power flow control strategy based on the grid and EV owners’ requirements is developed. The new control scheme is studied through numerical simulation using Simulink and PSCAD/EMTDC. The results have demonstrated that the proposed V2G technique can be effective for grid power quality improvement.
Risks associated with antidepressants in patients with hypertension during pregnancy: a retrospective cohort study
Authors: Weernink Marlieke Oude , Damer Elvera , Edens Mireillie , Bijvank Bas Nij , Hemels Marieke , Horst Peter Ter 
Published: November 2023

Abstract
Objective
In a cohort of pregnant women using antihypertensive drugs, we compared exposure to antidepressants versus no exposure and the possible association with birth weight, APGAR scores, NICU admission, and maternal admission to an obstetrical intensive care unit (OHC). It was hypothesized that pregnant women with hypertensive disorders using antidepressants are at greater risk of complications.
Setting
A retrospective cohort study in a general teaching hospital in Zwolle, in the Middle-Northern part of The Netherlands.
Participants
We selected 77 pregnancies eligible for the exposed group and 293 eligible for the reference group. Of those, we excluded patients because of twin pregnancy, stillbirth, patients who discontinued antidepressants during pregnancy, and termination of the pregnancy not related to hypertension or mental health problems during pregnancy.
Finally, 58 pregnancies in the exposed group and 273 pregnancies in the reference group met all inclusion and exclusion criteria.
Outcome
We compared the neonate’s birthweight between the exposed to antidepressants group and the reference group as the primary outcome. Secondary outcomes were the APGAR score at 1 and 5 minutes and obstetric high care (OHC) admission of the mother and neonatal intensive care unit (NICU) admission of the child.
Results
We found no differences in birth weight in neonates of mothers with hypertensive disorders and whether or not to use antidepressants. Besides a possible higher risk of admission to an OHC in women with hypertension-complicated pregnancies using antidepressants, we found no other maternal or neonatal risks in this population.
Conclusions
We found no additional maternal or neonatal risks of using antidepressants prescribed to women with hypertension disorders during pregnancy.
Unmet need for contraception among married women in the Kyrgyz Republic from the 2006, 2014 and 2018 Multiple Indicator Cluster Survey: A cross- sectional study
Authors: Dolonbaeva Zuura , Inthaphatha Souphalak , Dzhangaziev Bakyt , Ismailov Mederbek , Nishino Kimihiro , Hamajima Nobuyuki , Yamamoto Eiko 
Published: November 2023

Abstract
Background
This study aims to investigate the prevalence of an unmet need for contraception from 2006 to 2018, and examine the factors associated with an unmet need for contraception among married women over the course of 12 years in the Kyrgyz Republic.
Methods
This study employed three datasets from the Multiple Indicator Cluster Survey 2006, 2014, and 2018. There were 9,229 women aged 15–49 who were married and fecund included in this study. Logistic regression was employed to estimate the relationship of an unmet need for contraception with other factors. A P value < 0.05 was set as statistically significant.
Results
The prevalence of an unmet need for contraception was 19.9% in 2006, 20.4% in 2014, and 22.5% in 2018. Intrauterine devices were the prominent contraceptive method of usage among Kyrgyz women, but the trend of usage drastically declined over time. From 2006 to 2018, factors associated with unmet need for contraception included women’s age, area of residence, mother tongue of household head, age of husband, and number of children ever born.
Conclusion
The unmet need for contraception among married Kyrgyz women slightly increased, and the trend of contraception usage declined from 2006 to 2018. Sexual health education for young people and youth-friendly services should be promoted. An effective and reliable supply chain, including financing, forecasting, and procurement for contraception commodities, should be prioritized and strengthened. Regular supportive supervision visits are essential to improve the knowledge and skills of healthcare providers, and to sustain intrauterine device service as a contraceptive choice for Kyrgyz women.
Microsurgical Thrombectomy: where the ancient art meets the new era
Authors: Ota Nakao , Benet Arnau , Kusdiansah Muhammad , Miyoshi Norio , Haraguchi Kenichi , Noda Kosumo , Lawton Michael T , Tanikawa Rokuya 
Published: November 2023

Abstract
Background
Mechanical thrombectomy (MT) is the leading treatment for acute large vessel occlusion (LVO). However, surgical thrombectomy (ST) may have a role in well selected LVO patients where MT failed to re-establish flow, the endovascular route is inaccessible, or where MT is a financially prohibitive or absent option(developing and poor countries). We compared the efficacy and efficiency between ST and MT, and described our operative experience and its potential application in the developing world.
Methods
Clinical outcomes, procedural times and efficacy of treatment were compared between the MT and ST of acute LVO between 2012 and 2022. Propensity score-matched analysis was also conducted to compare MT and ST.
Results
One-hundred nine patients fulfilled the study criteria (77MTs vs 32STs). Factors driving outcome were age (aOR:0.95, 95%CI, 0.91-0.98), hemisphere side (aOR:0.38, 95%CI, 0.15-0.96), DWI-ASPECT (aOR:1.39, 95%CI, 1.09-1.77) at presentation by the multivariate analysis. Times from door-start of procedure(P=0.45), and start of procedure-recanalization(P=0.13) were similar between treatment options. Propensity score-matched analysis found no significant difference for 2 treatment methods about time of door to recanalization(P=0.155) and outcome(P=0.221).
Conclusions
The prognosticator of thrombectomy for acute LVO in patients with successful recanalization were age, affected hemisphere side and DWI-ASPECT score. Our evidence shows that the efficacy of ST is similar to that of MT. There should be a place of ST for cases of mechanical failure or tandem cervical ICA and MCA occlusion. ST may be a temporizing LVO treatment option in healthcare systems where MT is inexistent or financially prohibitive to patients.
Light-triggered conformational changes of an animal-like cryptochrome tracked by native time-resolved IMS-MS
Authors: Morgner Nina , Zangl René , Soravia Sejla , Saft Martin , Löffler Jan Gerrit , Schulte Jonathan , Rosner Christian , Bredenbeck Jens , Essen Lars 
Published: November 2023

Abstract
Cryptochromes (CRYs) belong to the class of blue light photoreceptors and are responsible for various light-triggered functions in the circadian rhythm upon excitation of their inbuilt flavin cofactor. They are functionally distinct to the evolutionarily related photolyases, which mediate light-driven repair of UV induced DNA damages. Despite these functional differences they share a high degree of structural and sequence homology. A hallmark of cryptochromes is their flexible carboxyl-terminal extension (CTE), whose structure and function as well as the details of its interaction with the photolyase homology region (PHR) are not yet fully understood and differs among different cryptochromes types. In this study we investigate the animal-like cryptochrome from Chlamydomonas reinhardtii (CraCRY). Here, we focus on the highly conserved C-terminal domain harboring the FAD chromophore, to study the effect of single mutations on the structural transition of the C-terminal helix α22 and the attached CTE upon lit-state formation. By coupling a high-power LED, mounted in the source region to an ion mobility mass spectrometer, we show that D321, the putative proton acceptor of the terminal proton-coupled electron transfer event from Y373, is essential for triggering the large-scale conformational changes of helix α22 and the CTE in the lit state.