bluuebunny/crossref_metadata_2025_split huggingface dataset:
A teratoproteomics analysis: Heat shock protein 70 is upregulated in mouse forelimb bud by methoxyacetic acid treatment
Authors: Ruyani Aceng , Sudarwati Sri , Sutasurya Lien A. , Sumarsono Sony H. , Kim Dong‐Jo , Chung Jun Ho 
Published: June 2005

AbstractBACKGROUNDMethoxyacetic acid (MAA) causes fetal limb abnormalities when the substance is administrated on gestation day (GD) 11 in mice. Limb abnormalities are caused mainly by extensive cell death in the mesoderm of the limb plate. This investigation focused on identifying a protein that is linked with mouse limb teratogenicity.METHODSA single dose of MAA at 10 mmol/kg body weight was administered by gavage on GD 11; controls were administered vehicle only. Dams were killed by cervical dislocation 4 hr after treatment and forelimb buds were isolated from both the control and treated embryos. Proteins in forelimb buds GD 11 + 4 hr were precipitated out using 40–60% ammonium sulfate and were then analyzed by 2D SDS‐PAGE. Excised protein spots were identified by mass spectrometry and amino acid internal sequence analysis. Identified protein was further confirmed by Western blotting.RESULTSTwo‐dimensional gel analysis indicated that 1 protein spot of 81.7 kDa/pI 7.3 was overexpressed, and the protein matched heat shock protein 70 (HSP70; accession no. P08109, SwissProt).CONCLUSIONSThe results suggest that MAA, when administered to pregnant mice, upregulates HSP70 in the forelimb buds. Birth Defects Research (Part A), 2005. © 2005 Wiley‐Liss, Inc.
Production of fuculose‐1‐phosphate aldolase using operator–repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations
Authors: Durany Olga , Bassett Philip , Weiss Amanda M.E. , Cranenburgh Rocky M. , Ferrer Pau , López‐Santín Josep , de Mas Carles , Hanak Julian A.J. 
Published: June 2005

AbstractWe report a novel application for the operator–repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. © 2005 Wiley Periodicals, Inc.
Stable hybridoma cultivation in a pilot‐scale acoustic perfusion system: Long‐term process performance and effect of recirculation rate
Authors: Dalm Marcella C.F. , Jansen Menno , Keijzer Timo M.P. , van Grunsven Wout M.J. , Oudshoorn Arthur , Tramper Johannes , Martens Dirk E. 
Published: June 2005

AbstractPerfusion systems have the possibility to be operated continuously for several months. It is important that the performance of the cell retention device does not limit the operation time of a perfusion process used in the production of active pharmaceutical ingredients. Therefore, the aim of this study was to investigate the reliability and long‐term stability of an acoustic perfusion process using the 200 L/d BioSep. As the BioSep is an external device, it is possible that dependent on the recirculation rate nutrient gradients occur in the external loop, which could affect the cell metabolism. Therefore, the effect of possible nutrient gradients on cell metabolism, viability and productivity was studied by varying the recirculation rate. In this study, it is shown that a perfusion process using a pilot‐scale acoustic cell‐retention device (200 L/d) is reliable and simple to operate, resulting in a stable 75‐day cultivation of a hybridoma cell line producing a monoclonal antibody. The recirculation rate had a significant effect on the oxygen concentration in the external loop, with oxygen being depleted within the cell‐retention device at recirculation rates below 6 m3/mreactor3·d (=600 L/d). The oxygen depletion at low circulation rates correlated with a slightly increased lactate production rate. For all other parameters no effect of the recirculation rate was observed, including cell death measured through the release of lactate dehydrogenase and specific productivity. A maximum specific productivity of 12 pg/cell · d was reached. © 2005 Wiley Periodicals, Inc.
Silyl Anions or Silylenoids?—A DFT Study of Silyllithium Compounds with π‐Donating Substituents
Authors: Flock Michaela , Marschner Christoph 
Published: June 2005

AbstractGeometry optimizations at the B3LYP/6‐31+G(d) level for a set of X(SiH3)MeSiLi molecules (X=F, OH, NH2, Cl, SH, and PH2) show that the tetrahedral structure prevails in polar solutions; however, it readily isomerizes into a silylenoid with energy barriers of less than 15 kJ mol−1. Inverted structures, which predominate in the gas phase, could not be located in solution. Configuration inversion is unfavorable, with energy barriers between 80 and 220 kJ mol−1. The α elimination into a silylene moiety and the corresponding LiX is only likely to occur in solution, particularly for X=Cl and SH.
The role of anxiety in prostate carcinoma
Authors: Dale William , Bilir Pinar , Han Misop , Meltzer David 
Published: June 2005

AbstractAlthough the impact of anxiety on patients with some types of cancer is well recognized, to the authors knowledge its impact on patients with prostate carcinoma has not been studied as thoroughly. The authors conducted a systematic review of the medical literature for high‐quality articles that quantified anxiety levels in men with prostate carcinoma and identified 29 articles. Using the clinical timeline of prostate carcinoma to organize the articles, cross‐sectional studies that reflected anxiety prevalence in populations and longitudinal studies that reflected changes in anxiety over time were identified. Anxiety appeared to fluctuate over the clinical timeline in response to stressors and uncertainty (such as at the time of screening and/or biopsy), rising before these times and falling afterward. Although anxiety levels in men age > 55 years who were at risk for prostate carcinoma were modest (10–15%), multiple studies found that these levels were substantially higher in men who presented for screening (> 50%), and “seeking peace of mind” was the motivation cited most frequently for pursuing screening. Most studies demonstrated a significant decrease in anxiety levels after a normal screening or biopsy result, although the proportion of men who remained anxious afterward did not fall to baseline levels (20–36%). Men who presented for prostate‐specific antigen monitoring after treatment had elevated anxiety levels at the time of testing (23–33%). Many years after therapy for localized disease, anxiety levels were lower after prostatectomy (23%) compared with the levels after watchful waiting (31%). Cancer 2005. © 2005 American Cancer Society.
Couple empowerment in divorce: A comparison of mediated and nonmediated outcomes
Authors: Bailey Jo Daugherty , Robbins Susan P. 
Published: June 2005

AbstractThis article examines whether empowerment through mediation actually produces tangible outcomes for divorcing couples in a random selection of 120 mediated and nonmediated divorce cases in Harris County, Texas. Data analysis supports the hypothesis: mediated cases result in more changes to the state‐ordered possessory arrangement governing access to children than nonmediated cases. A discussion of the significance of the findings for mediators is included.
Using the “mediatable frame” to define the problem in mediating a parenting plan
Authors: Benjamin Michael , Irving Howard H. 
Published: June 2005

AbstractTo explore the issue of defining the problem in family mediation, the authors compare rational and therapeutic models of mediation, focusing on their therapeutic family mediation (TFM) model and introducing and discussing the notion of the “mediatable frame.” They offer case history material and discuss its implications for theory and practice.
Inhibition of ultrasonic vocalizations by beta‐adrenoceptor agonists
Authors: Blumberg Mark S. , Johnson Eric D. , Middlemis‐Brown Jessica E. 
Published: June 2005

AbstractInfant rat ultrasonic vocalizations (USVs) are widely believed to result from the induction of an emotional state of anxiety or distress. This perspective, however, is not easily reconciled with the demonstration by W. J. Farrell and J. R. Alberts 2000 that norepinephrine, a nonselective beta‐adrenoceptor agonist with anxiogenic properties, inhibits production of USVs. Here, Farrell and Alberts' finding was replicated and extended with 12‐day‐old rats using a conventional isolation paradigm. First, treatment with norepinephrine (1 mg/kg) significantly inhibited ultrasound production while also increasing body temperature. Next, treatment with the beta‐2 agonist terbutaline (1 mg/kg) and the beta‐3 agonist CL‐316243 (1 mg/kg), but not the beta‐1 agonist dobutamine (1 mg/kg), inhibited ultrasound production; only CL‐316243 increased body temperature. The unexpected inhibition of USVs by terbutaline, a potent bronchodilator, was replicated using a slightly modified procedure; again, body temperature was unaffected by terbutaline administration. In no experiment was inhibition of USVs related to changes in motor activity. Altogether, these results suggest either that ultrasound production is not a valid indicator of anxiety or that anxiety in infant rats is produced by neuropharmacological mechanisms that differ fundamentally from those in adults. © 2005 Wiley Periodicals, Inc. Dev Psychobiol 47: 66–76, 2005.
Latent inhibition in the developing rat: An examination of context‐specific effects
Authors: Yap Carol S. L. , Richardson Rick 
Published: June 2005

AbstractLatent inhibition (LI) refers to the reduction in conditioned responding when the conditioned stimulus (CS) is preexposed prior to CS–unconditioned stimulus pairings. Experiment 1a demonstrated that preexposure to an odor CS prior to odor–shock pairings markedly reduced conditioned freezing in 25‐day‐old rats; however, this LI effect was observed only if odor preexposure and odor–shock pairings occurred in the same context (i.e., LI was context‐specific at this age). The results of Experiment 1b showed that 18‐day‐olds also exhibited LI, but this effect was not context‐specific at this age. In Experiment 2, rats were preexposed to the odor at 18 days of age and given odor–shock pairings at 25 days of age. These rats exhibited context‐specific latent inhibition, suggesting that 18‐day‐old rats encoded the preexposure context. In Experiment 3, all parameters were identical to Experiment 2, with the exception that odor–shock pairings were given at ∼PN18 and testing occurred at ∼PN25. These rats exhibited latent inhibition at test, but this effect was not context‐specific. The results of this study suggest that (a) PN18 rats can exhibit latent inhibition, and (b) the expression of context‐specific latent inhibition depends on the age at which conditioning occurs. © 2005 Wiley Periodicals, Inc. Dev Psychobiol 47: 55–65, 2005.
Detection of geometric, but not topological, spatial transformations in 6‐ to 12‐month‐old infants in a visual exploration paradigm
Authors: Lew Adina R. , Foster Kirsty A. , Bremner J. Gavin , Slavin Simon , Green Michael 
Published: June 2005

AbstractSeveral theories of spatial orientation propose that the geometry of an environment plays a privileged role in reorientation, relative to relations between individual landmarks. Infants (N = 90) in three age groups (6, 8½, and 12 months) experienced three conditions: topological, geometric, and control. A round room contained four distinctive objects in a rectangular arrangement on the inside periphery. Infants were familiarized to the array prior to a 2‐min test period. In the topological condition, two objects were switched. In the geometric condition, the objects were moved to form an irregular quadrilateral. In the control condition, the array remained unchanged. Infants of 8½ months and over visually explored significantly more in the geometric condition only. An initial study with adults found greater visual exploration in both geometric and topological conditions. These results are discussed in the context of current theories of spatial orientation. © 2005 Wiley Periodicals, Inc. Dev Psychobiol 47: 31–42, 2005.
The First X‐ray Characterized Monosubstituted Ferrocenyl Azacrown Chalcone: Focus on Its Calcium Interaction/Electrochemical Detection Studies
Authors: Delavaux‐Nicot Béatrice , Maynadié Jérôme , Lavabre Dominique , Lepetit Christine , Donnadieu Bruno 
Published: June 2005

AbstractThe first X‐ray characterization of an azacrown ferrocenyl chalcone [(C5H5)Fe(C5H4COCH=CHC6H4‐p‐aza‐15‐crown‐5)] (2) is reported. Its cation electrochemical detection capabilities have been evaluated and its behaviour towards protonation and calcium addition has been thoroughly examined. The uncommon ligand–Ca2+ interaction process involves three species of different stoichiometry in equilibrium in solution. Their association constants have been calculated. These species are formed by interaction of calcium with both the azacrown and CO functions of compound 2, as evidenced by NMR and IR spectroscopy. The theoretical MESP analysis also suggests that, contrary to its N‐ethyl‐substituted homologue [(C5H5)Fe(C5H4COCH=CHC6H4NEt2)] (1a), both coordination sites of 2 are involved in this interaction process. The study highlights that the CO group of 2 improves the selectivity of the cation electrochemical detection whencompared to the known compound [(C5H5)Fe(C5H4CH=CHC6H4‐p‐aza‐15‐crown‐5)]. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Multi‐Channel Receptors and Their Relation to Guest Chemosensing and Reconfigurable Molecular Logic Gates
Authors: Jiménez Diego , Martínez‐Máñez Ramón , Sancenón Félix , Ros‐Lis José V. , Soto Juan , Benito Ángel , García‐Breijo Eduardo 
Published: June 2005

AbstractThe synthesis and characterisation of a family of multi‐channel receptors containing crown‐cation binding sites anchored to a 1‐aminophenyl‐1,2,2‐tricyanoethylene group is reported. The ligands L1–L6 bear a 1‐aminophenyl‐1,2,2‐tricyanoethylene scaffolding which is simultaneously a redox‐active group (showing reduction processes at moderately modest potentials) and an acceptor moiety in the 1‐aminophenyl‐1,2,2‐tricyanoethylene chromophore. Additionally, dyes L1–L6 also show fluorescence emission. The colour variation of L1–L6 in acetonitrile in the presence of the metal cations Ag+, Cd2+, Cu2+, Fe3+, Hg2+, Pb2+ and Zn2+ has been studied. Selective hypsochromic shifts were found for the systems L4‐Pb2+, L5‐Hg2+ and L6‐Hg2+. For Hg2+ with L5 and L6 and Pb2+ with L4, emission fluorescence enhancements most likely associated with metal coordination to the anilinium nitrogen were observed. The electrochemical behaviour of receptors L1–L6 was studied in acetonitrile with platinum as working electrode and [Bu4N][BF4] as supporting electrolyte. This family of receptors shows a one‐electron reversible reduction process at around –0.70 V vs. SCE, attributed to the reduction of the tricyanovinyl group. Additionally, all the receptors also show the oxidation of the anilinium moiety at about 1.2–1.4 V vs. SCE. Significant anodic shifts of both reduction and oxidation waves were found in the presence of certain metal cations. The relationship of these multiple‐channel signalling receptors with guest chemosensing and reconfigurable molecular‐based logic gates is discussed. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
The Hexakis(thiocyanato)ferrate(III) Ion: a Coordination Chemistry Classic Reveals an Interesting Geometry Pattern for the Thiocyanate Ligands
Authors: Addison Anthony W. , Butcher Raymond J. , Homonnay Zoltán , Pavlishchuk Vitaly V. , Prushan Michael J. , Thompson Laurence K. 
Published: June 2005

Abstract(NMe4)3[Fe(NCS)6] crystallizes from ethanol in the monoclinic space group C2/c. Two different types of complex ions are contained in the unit cell, though both possess exclusively N‐coordination of the thiocyanate ligands. In one ion, the thiocyanate ligands are all essentially linearly bound, with an Fe–N–C angle of 174 ± 4°, while in the other, there are two cis‐thiocyanate ligands with a notably small Fe–N–C angle of 146.5°. The EPR and Mössbauer results show that all the iron(III) centers maintain the high‐spin state down to 80 K, while the magnetic susceptibility confirms this to 2 K. Infrared and Mössbauer spectra provide evidence for an unusually “soft” lattice. Traditional criteria for interpretation of the infrared frequencies are not strictly applicable for this compound. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
[1+1] Asymmetric Compartmental Macrocycles Bearing a Pendant Arm and Related s,f‐Heterodinuclear Complexes Containing Lanthanide(III) and Sodium Ions
Authors: Tamburini Sergio , Casellato Umberto , Bertolo Laura , Alessandro Vigato Pietro 
Published: June 2005

Abstract[1+1] Asymmetric compartmental macrocycles and/or related mononuclear, homodinuclear and sodium(I)‐lanthanide(III) heterodinuclear complexes are reported and their properties investigated by SEM‐EDS microscopy and 1H, 13C and 23Na NMR spectroscopy. These ligands bear a pendant arm, represented by a ‐CH2COOH (H3LA), ‐CH2C5H4N (H2LB) or‐CH2C6H4OH (H3LC) group bonded to the aminic nitrogen, and contain an O2O3 crown‐ether‐like chamber and an N3XO2 (where X = O, N) Schiff‐base chamber. It was found that the lanthanide(III) ion resides in the N3XO2 chamber while the sodium(I) ion fills the O2O3 crown‐ether‐like chamber, as ascertained for the complex [DyNa(LC)(PrOH)(Cl)] by a single‐crystal X‐ray structural investigation. The complex is monoclinic, space group P21/c, with a = 9.601(2), b = 12.927(2), c = 26.638(4) Å, β = 99.55(3)°. The dysprosium ion is seven‐coordinate in the N3O2 site, showing a pentagonal‐bipyramid‐coordination polyhedron and bonded, in the axial positions, to a chlorine ion and to the oxygen of the pendant phenolic group of the ligand. The sodium ion is six‐coordinate in the O3O2 site and bonded to the propyl alcohol oxygen. Furthermore, the potential use of these complexes as molecular probes for the selective recognition of specific metal ions has been tested. In particular, their ability to act as shift reagents towards Na+ was investigated by 23Na NMR spectroscopy. The relationship between the structure of these complexes in the solid state and in solution is reported. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Hemilabile Thioether Ligands Based on Pyrimidine and/or Pyridine Derivatives that Interconvert between N,S‐ and N‐Coordination in Congested Ruthenium(II) Complexes
Authors: Tresoldi Giuseppe , Baradello Laura , Lanza Santo , Cardiano Paola 
Published: June 2005

AbstractThe thioether ligands L [L = 2‐pyridylmethyl 2'‐pyridyl sulfide (L1), 2‐pyridylmethyl 2'‐pyrimidyl sulfide (L2) and 2‐pyridylmethyl 2'‐(4‐methylpyrimidyl) sulfide (L3)] react with cis‐[Ru(N,N‐diimine)2Cl2] {diimine = 2,2'‐bipyridine (bipy), di‐2‐pyrimidyl sulfide (dprs), 2,2'‐bis(5‐ethylpyrimidyl) sulfide (5edprs)} to give compounds [Ru(N,N‐diimine)2L][PF6]2 {diimine = bipy, L =L1 (1), L2 (2), L3 (3); diimine = dprs, L = L1 (4); diimine = 5edprs, L = L1 (5); diimine = dprs, L = L2 (6), L = L3 (7); diimine = 5edprs, L = L2 (8), L = L3 (9)}. NMR investigations show that these potentially tridentate ligands act as N,S‐bidentate species, to form a five‐membered RuSCCN(Ru–N) ring, and in certain cases, as N‐monodentate species coordinated to the ruthenium through the 2‐pyridylmethyl group. The N,S‐chelated species contain chiral sulfur and ruthenium atoms with (R) and (S), and Δ and Λ configurations, respectively. Two invertomers and two sets of NMR signals in the slow‐exchange region are expected. However, the low‐temperature 1H NMR spectra show that sulfur inversion is fast. The variable‐temperature 1H NMR spectra allow two species to be observed (6a + 6b, 7a + 7b, 8a + 8b and 9a + 9b), which exhibit different abundances. In the minor species (6b, 7b, 8b, and 9b), L2 or L3 exhibits an N‐monodentate coordination, while in the major species, the usual N,S‐coordination. At low temperatures, the population ratio is about 85:15, while when the temperature increases, the abundance of the minor species grows rapidly. The one‐dimensional band‐shape analysis of the exchanging methylene proton signals shows that the energy‐barrier for the interchange process (ΔG#298) for 6a + 6b, 7a + 7b, 8a + 8b, and 9a + 9b is practically the same (ca. 59.5 kJ·mol–1), while the ΔS# values are negative or near to zero. The possible mechanisms for the process are discussed. The NMR spectroscopic findings strongly support the formation of an N,N‐chelated labile intermediate. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
Synthesis and Characterization of Radical Cations Derived from Mono‐ and Biferrocenyl‐Substituted 2‐Aza‐1,3‐butadienes: A Study of the Influence of an Asymmetric and Oxidizable Bridge on Intramolecular Electron Transfer
Authors: Lloveras Vega , Caballero Antonio , Tárraga Alberto , Velasco M. Desamparados , Espinosa Arturo , Wurst Klaus , Evans David J. , Vidal‐Gancedo José , Rovira Concepció , Molina Pedro , Veciana Jaume 
Published: June 2005

AbstractThe synthesis and study of structural and electronic properties of mono‐ferrocenyl π‐conjugated complexes 5a–d, whose electronic characteristics have been systematically varied by introducing an electron‐donating or electron‐withdrawing substituent either at the 1‐position or at the 4‐position of the 2‐aza‐1,3‐butadiene moiety linked to the ferrocenyl unit, are presented. The structural and electronic properties of the homobimetallic complex 5f, with two ferrocene units linked through the asymmetric and oxidizable 2‐aza‐1,3‐butadiene bridge, is also reported. The crystal structures of complexes 5b, 5d, and 5f show a large degree of conjugation in this family of compounds. Complexes 5 show a rich electrochemical behavior due both to the oxidation of ferrocenyl units and the 2‐aza‐1,3‐butadiene bridge, as revealed by cyclic voltammetry. Radical cations 5+· were prepared from 5 by coulometric oxidations following their generation by absorption spectroscopy. The electronic properties of all reported neutral and oxidized π‐conjugated complexes have been investigated by means of UV/Vis–near‐IR, EPR and 57Fe Mössbauer spectroscopy. The detailed study of mono‐oxidized species 5a+·–5f+· has permitted the determination of the influence of an asymmetric bridge with an electroactive character on the intramolecular electron transfer (IET) phenomenon, thus demonstrating that the 2‐aza‐1,3‐butadiene bridge promotes the IET between the two metallic units of 5f+· through two different pathways. The experimental data and conclusions are supported by DFT computations (B3LYP/3‐21G*) and time‐dependent DFT methods. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)