bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Everlasting Star
Authors: Pegg Mark Gregory 
Published: January 2024

Abstract
This chapter details the life and experiences during the years 722-824, such as the life of Huneberc, a young English nun in the monastery of Heidenheim. It cites how the history of the eighth and early ninth centuries primarily consists of the seeming inevitability of Charles and his new Roman Empire. The consequences of Charles' action reverberated throughout the West. Beyond the conquests and capitularies, the idea and reality of a Western Roman Empire and the notion of a corrected Latin Christianity endured throughout the years. The chapter explains the type of Christendom that saw paganism or heresy in every environment possible.
The Harrying of the Heathen
Authors: Pegg Mark Gregory 
Published: January 2024

Abstract
This chapter cites the appearance of the Northmen as a transformative moment in the West in the late eighth century. Northmen's appearance resulted in their continued raiding, conquest, and colonization for centuries. Moreover, the pagan holy warfare, which was embodied by longships, was shocking and destabilizing from the North Atlantic to the Mediterranean. The chapter explains that the inherent fractiousness of the Franks on the Rhine was aggravated and hastened by the Northmen. Despite the violence of Danish, Swedish, and Norwegian pirate fleets, Northmen helped reinforce and create a more universal sense of Christianity transcending the much smaller parochial Christendoms.
Only Death Wakes Us from Dreaming
Authors: Pegg Mark Gregory 
Published: January 2024

Abstract
This chapter looks into the life experiences of medieval Europe. It explores Giovanni Boccaccio's Decameron, which raised debates on whether the great plague descended upon humanity as a punishment from God or an influence from the stars and planets. Giovanni wrote most of the Decameron during the height of the plague, vividly using the horrific reality of the pestilence as a way of opening and so framing his fictional comedy. The chapter reflects on how small and fragile western Christendoms transformed into an all-embracing Latin Christendom. It also notes that people struggled with their identities as pagans, Jews, Muslims, and especially as Christians throughout the centuries.
Ferrostatin‐1 inhibits ferroptosis of vascular smooth muscle cells and alleviates abdominal aortic aneurysm formation through activating the SLC7A11/GPX4 axis
Authors: He Xin , Xiong Yunchuan , Liu Yu , Li Yaozhen , Zhou Haiyang , Wu Kemin 
Published: January 2024

AbstractFerroptosis, a type of iron‐catalyzed necrosis, is responsible for vascular smooth muscle cell (VSMC) death and serves as a potential therapeutic target for alleviating aortic aneurysm. Here, our study explored the underlying mechanism of ferroptosis affecting VSMC functions and the resultant formation of AAA using its inhibitor Ferrostatin‐1 (Fer‐1). Microarray‐based gene expression profiling was employed to identify differentially expressed genes related to AAA and ferroptosis. An AAA model was established by angiotensin II (Ang II) induction in apolipoprotein E‐knockout (ApoE−/−) mice, followed by injection of Fer‐1 and RSL‐3 (ferroptosis inducer). Then, the role of Fer‐1 and RSL‐3 in the ferroptosis of VSMCs and AAA formation was analyzed in Ang II‐induced mice. Primary mouse VSMCs were cultured in vitro and treated with Ang II, Fer‐1, sh‐SLC7A11, or sh‐GPX4 to assess the effect of Fer‐1 via the SLC7A11/GPX axis. Bioinformatics analysis revealed that GPX4 was involved in the fibrosis formation of AAA, and there was an interaction between SLC7A11 and GPX4. In vitro assays showed that Fer‐1 alleviated Ang II‐induced ferroptosis of VSMCs and retard the consequent AAA formation. The mechanism was associated with activation of the SLC7A11/GPX4 pathway. Silencing of SLC7A11 or GPX4 could inhibit the ameliorating effect of Fer‐1 on the ferroptosis of VSMCs. In vivo animal studies further demonstrated that Fer‐1 inhibited Ang II‐induced ferroptosis and vessel wall structural abnormalities in AAA mouse through activation of the SLC7A11/GPX4 pathway. Fer‐1 may prevent AAA formation through activation of the SLC7A11/GPX4 pathway.
Coiled‐coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK‐NF‐κB signaling pathway
Authors: Qian Zhenzhen , Zhao Huizi , Zhang Yuan , Wang Zhonghao , Zeng Fanle , Zhu Yan , Yang Yaru , Li Jun , Ma Taotao , Huang Cheng 
Published: January 2024

AbstractIncreasing evidence has demonstrated that the expression of coil domains containing 25 (CCDC25) in various malignancies is abnormally high. However, the potential regulatory role and mechanism of CCDC25 in the development of clear cell renal cell carcinoma (ccRCC) are still unclear. In this experiment, we combined in vitro experiments such as wound healing, CCK8, and transwell assay with in vivo experiments on tumor formation in nude mice to evaluate the effect of CCDC25 on the proliferation, migration, and invasion of renal cancer cells. In addition, we also used Western blotting and qPCR to evaluate the role of CCDC25 in activating the integrin‐linked kinase (ILK)‐NF‐κB signaling pathway. Here, we demonstrate that compared to normal tissues and cell lines, CCDC25 is overexpressed in both human ccRCC tissues and cell lines. After CCDC25 knockdown, it has obvious inhibitory effect on the proliferation, migration, and invasion of cancer cells in vitro and in vivo. In contrast, CCDC25 overexpression promotes these effects. Additionally, we also discovered that CCDC25 interacts with ILK and coordinates the activation of the NF‐κB signaling pathway downstream. Generally, our study suggests that CCDC25 plays a vital role in the development of ccRCC, which also means that it may be a potential therapeutic target for ccRCC.
European Society of Anaesthesiology and Intensive Care consensus document on sustainability
Authors: Gonzalez-Pizarro Patricio , Brazzi Luca , Koch Susanne , Trinks Alexandra , Muret Jane , Sperna Weiland Niek , Jovanovic Gordana , Cortegiani Andrea , Fernandes Tiago David , Kranke Peter , Malisiova Anna , McConnell Paul , Misquita Lucia , Romero Carolina S. , Bilotta Federico , De Robertis Edoardo , Buhre Wolfgang ,   the Sustainability National Representatives
Published: January 2024

Climate change is a defining issue for our generation. The carbon footprint of clinical practice accounts for 4.7% of European greenhouse gas emissions, with the European Union ranking as the third largest contributor to the global healthcare industry's carbon footprint, after the United States and China. Recognising the importance of urgent action, the European Society of Anaesthesiology and Intensive Care (ESAIC) adopted theGlasgow Declaration on Environmental Sustainabilityin June 2023. Building on this initiative, the ESAIC Sustainability Committee now presents a consensus document in perioperative sustainability. Acknowledging wider dimensions of sustainability, beyond the environmental one, the document recognizes healthcare professionals as cornerstones for sustainable care, and puts forward recommendations in four main areas: direct emissions, energy, supply chain and waste management, and psychological and self-care of healthcare professionals. Given the urgent need to cut global carbon emissions, and the scarcity of evidence-based literature on perioperative sustainability, our methodology is based on expert opinion recommendations. A total of 90 recommendations were drafted by 13 sustainability experts in anaesthesia in March 2023, then validated by 36 experts from 24 different countries in a two-step Delphi validation process in May and June 2023. To accommodate different possibilities for action in high- versus middle-income countries, an 80% agreement threshold was set to ease implementation of the recommendations Europe-wide. All recommendations surpassed the 80% agreement threshold in the first Delphi round, and 88 recommendations achieved an agreement >90% in the second round. Recommendations include the use of very low fresh gas flow, choice of anaesthetic drug, energy and water preserving measures, “5R” policies including choice of plastics and their disposal, and recommendations to keep a healthy work environment or on the importance of fatigue in clinical practice.Executive summaries of recommendations in areas 1, 2 and 3 are available as cognitive aids that can be made available for quick reference in the operating room.
Objective Quantification of Depth-of-Field Advantage in 3D Surgical Video System for Vitreoretinal Surgery: Safety and Efficacy in Macular Diseases
Authors: Zhang Xiang , Hu Hanling , Li Wenbo , Zhu Danni , Nie Zetong , Guo Haoxin , Wang Zhaoxiong , Li Xiaorong , Hu Bojie 
Published: January 2024

Purpose:
The objective of this study is to demonstrate, based on objective clinical indicators, the advantages of depth of field provided by the 3D surgical video system compared to the traditional microscope during vitrectomy for treating epiretinal membranes（ERM) or macular holes(MH).

Methods:
A total of 38 patients were included in this study and randomly assigned to either the 3D surgical video group or the conventional microscope group. Surgical parameters such as the Focal plane adjustment frequency (FPAF), membrane peeling time, and number of attempts to peel the membrane were recorded for each patient. Additionally, patients were followed up for 3 months postoperatively.

Results:
No significant differences in age, gender, operated eyes, or follow-up rates between the groups. The 3D group had significantly lower Focal Plane Adjustment Frequency (FPAF) in macular hole surgery and epiretinal membrane surgery . No significant differences in peeling maneuvers, time, or total surgical time. Postoperative follow-up data showed no significant differences.

Conclusion:
In conclusion,the 3D surgical video system exhibits potential advantages in depth of field.The 3D surgical video system is a safe and effective technology in vitrectomy for macular diseases.
Persistent Subretinal Fluid after Vitrectomy for Macular Hole-Associated Retinal Detachment
Authors: Cheng Feng-hui , Lin Hong-hao , Zhu Yan-chun , Chen Wen-juan , Wu Chen-xin , Wu Yu-yang , Zhang Zong-duan , Pan Qin-tuo 
Published: January 2024

Purpose:
To evaluate the incidence, associated factors and outcome of persistent subretinal fluid (SRF) after vitrectomy for macular hole-associated retinal detachment (MHRD).

Methods:
A total of 158 eyes from 156 patients with MHRD who achieved macular hole closure following primary vitrectomy were included in the analysis. Persistent SRF was defined as the presence of SRF for more than 1 month after first surgery. Preoperative and postoperative parameters were analyzed for their relationship with SRF development.

Results:
Persistent SRF was observed in 19 eyes (12.0% of 158) postoperatively. Seven eyes (36.8% of 19) with persistent SRF eventually displayed complete absorption during follow-up. Univariate analysis revealed that eyes with persistent SRF were statistically associated with internal limiting membrane(ILM) inverted flap, duration of symptoms, tamponade (perfluoropropane /silicone oil: 14/5 vs 35/104, P<0.001), and MHRD subtype (Type 1 /Type 2 /Type 3: 15/4/0 vs 60/40/39, P=0.003). In multivariate analysis, only ILM inverted flap(odds ratio, 15.778, 95% confidence interval, 3.170-78.523; P=0.001) was positively associated with persistent SRF. There were no significant differences in best-corrected visual acuity improvement(P=0.425) between the SRF involved foveal and without involved foveal groups, and no significant differences between the SRF complete absorption and incomplete absorption groups.

Conclusions:
Absorption of persistent SRF may be more difficult in MHRD eyes than in ordinary rhegmatogenous retinal detachment eyes. The ILM inverted flap in MHRD was associated with a greater likelihood of persistent SRF. The location and incomplete absorption of persistent SRF did not appear to be associated with the final visual outcome.
Assessing the Physical and Psychological Well-being of Construction Workers During the COVID-19 Pandemic
Authors: Tobia Loreta , Muselli Mario , Mastrangeli Giada , Cofini Vincenza , Di Marcello Giuseppina , Necozione Stefano , Fabiani Leila 
Published: January 2024

Objective
This prospective study aimed to assess the physical and psychological health of construction workers and examine the relationship between their well-being and the preventive measures implemented against SARS-CoV-2.

Methods
During occupational visit, the workers were invited to participate the survey. Two measurements were made and compared: after the Italian lockdown and on the second wave of COVID-19 pandemic.

Results
Fifty-three workers participated in the study. The percentage of workers who considered themselves in good health increased from 66.0% to 81.1%, and there was a significant decrease in the 12-item General Health Questionnaire score (16.8 vs 14.0, P = 0.0003). This reduction was associated with a higher perception of security by preventive measures.

Conclusions
The study highlights the importance of addressing the health and safety concerns of construction workers during the pandemic, and the positive impact of effective preventive measures.
The Relationship Between Employee Physical Activity Intensity and Workplace Burnout
Authors: Albedry Brandon , Marenus Michele W. , Chen Weiyun 
Published: January 2024

Objective
This study aimed to investigate employees’ physical activity (PA) levels and workplace burnout.

Methods
Participants, employed full-time, recruited through Prolific Academic, completed the Maslach Burnout Inventory and the International Physical Activity Questionnaire.

Results
An analysis of variance revealed a significant difference in burnout emotional exhaustion (F (2,516) = 3.55, P = 0.03) and personal accomplishment (F (2,516) = 4.23, P = 0.02) among three levels of PA groups, but not in burnout depersonalization. The moderate PA group scored significantly lower in burnout emotional exhaustion than the low PA group (P = 0.025). The low PA group scored significantly lower in the burnout personal accomplishment than the high PA group (P = 0.02) and the moderate PA group (P = 0.03).

Conclusions
Employees engaging in moderate PA showed an effective reduction in their burnout emotional exhaustion and promoted personal accomplishment.
Virtual Reality Simulation Integration in a Prelicensure Nursing Program
Authors: Willett Joanna , Adelman-Mullally Theresa , Ng Holly , Chung Seon Yoon 
Published: January 2024

Background:
Simulation, whether conducted live or through virtual technologies, provides students with experiential learning by replicating realistic clinical experiences that can develop learner competencies in a safe environment. Virtual reality simulation may provide the same level of effective and engaging experiential learning in a fully immersive learning environment without many of the limitations inherent to traditional live simulation environments, such as simulation equipment and designated labs.

Problem:
While there are vendor-provided resources and a new tool kit covering immersive technology simulation implementation in nursing, a lived example to contextualize the implementation process is missing from the literature.

Approach:
This article discusses the selection and implementation of virtual reality simulations in a nursing undergraduate program and lessons learned in the process.

Conclusions:
Nurse educators seeking such experiences for their students may benefit from the insights shared in this article.
Ensuring Advanced Practice Nursing Students Meet the 2023 Drug Enforcement Agency Requirements
Authors: Slater Tammy M. , Renda Susan , Peters Jessica , Mollenkopf Nicole , Rodney Tamar , Finnell Deborah S. 
Published: January 2024

Background:
All advanced practice nursing students in the doctor of nursing practice program at Johns Hopkins School of Nursing are required to complete 3 core courses (pathophysiology, pharmacology, and physical assessment). As of June 2023, the Drug Enforcement Agency (DEA) required all prescribers of controlled medications to attest to at least 8 hours of substance use training.

Purpose:
To quantify the amount of time advanced practice nursing students learn substance use–related content and engage in teaching/learning activities across the 3 courses as a basis for meeting the DEA requirement.

Methods:
Lead course faculty teaching identified content related to substance use disorders, including reading assignments and skill-building activities, and the minimum amount of time that students spend in those teaching/learning experiences.

Results:
Advanced practice nursing students complete a total of 14 hours of substance use–related content in the 3 core courses.

Conclusions:
This article describes substance use–related content and strategies that can be integrated in advanced practice nursing programs for students to meet the new DEA requirement.
An update on psychogenic nonepileptic seizures
Authors: Vijay Mithila , Reuber Markus 
Published: January 2024

Purpose of review
The understanding of psychogenic nonepileptic seizures (PNES) has advanced steadily over recent decades. This update summarizes new insights from the last three years.

Recent findings
The process of diagnosing PNES has shifted from the exclusion of epilepsy to one based on the recognition of typical clinical features. While the diagnosis cannot rely on any single feature in isolation, a range of semiological features characterising PNES are now recognised and a number of studies hint at the potential for machine learning and AI to improve the diagnostic process. Advances in data processing and analysis may also help to make sense of the heterogeneity of PNES populations demonstrated by recent studies focussing on aetiology and patient subgroups. It is now clear that PNES are associated with high rates of mental and physical comorbidities and premature death, highlighting that they are only one manifestation of a complex disorder extending beyond the nervous system and the seizures themselves.

Summary
PNES are now understood as a manifestation of dysfunction in interacting brain networks. This understanding provides an explanation for the psychopathological and semiological heterogeneity of PNES patient populations. New insights into medical comorbidities and increased rates of premature death call for more research into associated pathological processes outside the nervous system.
Pathogenic autoantibody internalization in myositis
Authors: Pinal-Fernandez Iago , Muñoz-Braceras Sandra , Casal-Dominguez Maria , Pak Katherine , Torres-Ruiz Jiram , Musai Jon , Dell’Orso Stefania , Naz Faiza , Islam Shamima , Gutierrez-Cruz Gustavo , Cano Maria Dolores , Matas-Garcia Ana , Padrosa Joan , Tobías-Baraja Esther , Garrabou Gloria , Aldecoa Iban , Espinosa Gerard , Simeon-Aznar Carmen Pilar , Guillen-Del-Castillo Alfredo , Gil-Vila Albert , Trallero-Araguas Ernesto , Christopher-Stine Lisa , Lloyd Thomas E. , Liewluck Teerin , Naddaf Elie , Stenzel Werner , Greenberg Steven A. , Grau Josep Maria , Selva-O’Callaghan Albert , Milisenda Jose C. , Mammen Andrew L. 
Published: January 2024

ABSTRACTObjectivesMyositis is a heterogeneous family of autoimmune muscle diseases. As myositis autoantibodies recognize intracellular proteins, their role in disease pathogenesis has been unclear. This study aimed to determine whether myositis autoantibodies reach their autoantigen targets within muscle cells and disrupt the normal function of these proteins.MethodsConfocal immunofluorescence microscopy was used to localize antibodies and other proteins of interest in myositis muscle biopsies. Bulk RNA sequencing was used to study the transcriptomic profiles of 668 samples from patients with myositis, disease controls, and healthy controls. Antibodies from myositis patients were introduced into cultured myoblasts by electroporation and the transcriptomic profiles of the treated myoblasts were studied by bulk RNA sequencing.ResultsIn patients with myositis autoantibodies, antibodies accumulated inside myofibers in the same subcellular compartment as the autoantigen. Each autoantibody was associated with effects consistent with dysfunction of its autoantigen, such as the derepression of genes normally repressed by Mi2/NuRD in patients with anti-Mi2 autoantibodies, the accumulation of RNAs degraded by the nuclear RNA exosome complex in patients with anti-PM/Scl autoantibodies targeting this complex, and the accumulation of lipids within myofibers of anti-HMGCR-positive patients. Internalization of patient immunoglobulin into cultured myoblasts recapitulated the transcriptomic phenotypes observed in human disease, including the derepression of Mi2/NuRD-regulated genes in anti-Mi2-positive dermatomyositis and the increased expression of genes normally degraded by the nuclear RNA exosome complex in anti-PM/Scl-positive myositis.ConclusionsIn myositis, autoantibodies are internalized into muscle fibers, disrupt the biological function of their autoantigen, and mediate the pathophysiology of the disease.
Genome assembly of the rare and endangered Grantham’s camellia,Camellia granthamiana
Authors:   Hong Kong Biodiversity Genomics Consortium, Hui Jerome H.L. , Chan Ting Fung , Chan Leo L. , Cheung Siu Gin , Cheang Chi Chiu , Fang James K.H. , Gaitan-Espitia Juan Diego , Lau Stanley C.K. , Sung Yik Hei , Wong Chris K.C. , Yip Kevin Y.L. , Wei Yingying , Law Sean T.S. , So Wai Lok , Nong Wenyan , Law Sean T.S. , Nong Wenyan , Lau David T.W. , Yip Ho Yin 
Published: January 2024

AbstractThe Grantham’s camellia (Camellia granthamianaSealy) is a rare and endangered tea species that is endemic to southern China, and was first discovered in Hong Kong in 1955. Despite its high conservation value, genomic resources ofC. granthamianaremain limited. Here, we present a chromosome-scale draft genome of the tetraploidC. granthamiana(2n = 4x = 60) using a combination of PacBio long read sequencing and Omni-C data. The assembled genome size is ∼2.4 Gb with most sequences anchored to 15 pseudochromosomes that resemble a monoploid genome. The genome is of high contiguity, with a scaffold N50 of 139.7 Mb, and high completeness with a 97.8% BUSCO score. Gene model prediction resulted in a total 76,992 protein-coding genes with a BUSCO score of 85.9%. 1.65 Gb of repeat content was annotated, which accounts for 68.48% of the genome. The Grantham’s camellia genome assembly provides a valuable resource for future investigations on its biology, ecology, phylogenomic relationships with otherCamelliaspecies, as well as set up a foundation for further conservation measures.