bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytochemical study: Fragmentation patterns of flavonoid‐C‐glycosides in the enriched flavonoids from corn silk using high‐efficiency ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry
Authors: Wang Yumei , Gu Meiling , Mao Jialin , Liu Jianhua , Fan Songjie , Zhang Honglian , Bu Hongzhou , Liu Qi 
Published: November 2023

AbstractFlavonoid‐C‐glycosides with various pharmacological actions were abundant in corn silk, and fragmentation in mass spectra of flavonoid‐C‐glycosides compounds was particularly important. This paper aimed to supervise the fragmentation patterns of flavonoid‐C‐glycosides in the enriched flavonoids from corn silk by high‐efficiency ultra‐high‐performance liquid chromatography combined with a quadrupole time‐of‐flight mass spectrometry approach. Therefore, the flavonoids were extracted by heating reflux and then purified based on D101 macroporous resin. Thirty‐five flavonoids were triumphantly identified in the purified product. Among them, 14 were flavonoid‐C‐glycosides, seven of them owned luteolin mother nuclei (luteolin‐C‐glycosides) and seven of them owned apigenin mother nuclei (apigenin‐C‐glycosides). Importantly, the diagnostic product ions of luteolin‐C‐glycosides including m/z (431, 413, 395, 369, 367, 313, and 299) and m/z (429, 411, 393, 367, 365, 311, and 297), and the characteristic product ions of apigenin‐C‐glycosides covered m/z (353, 339, and 299) and m/z (339, 327, and 309). In addition, diagnostic H2O neutral loss and glycosyl craking were familiar, and the difference values among product ions mainly contained 40, 60, 70, 80, 90, 100, and 120 Da. Thus, the fragmentations of luteolin‐C‐glycosides and apigenin‐C‐glycosides identified in corn silk were with proper regularity. The obtained fragmentation patterns provided rapid recognition and meaningful guidance for the purification study of flavonoid‐C‐glycosides.
Instructing Liquid‐Liquid Phase Separation Inside Membranous Protocells
Authors: Lv Xintao , Liu Jiawei , Song Peiyong , Zhao Li , Lin Yiyang 
Published: November 2023

AbstractThe bottom‐up fabrication of compartmentalized cell‐like entities represents a promising avenue for reconstituting hierarchical organization within cells and emulating life‐like behaviors. Integrating the creation of semipermeable membranes and membraneless artificial organelles has recently garnered significant attention, aiming to achieve cytomimetic properties. We briefly describe the concept of liquid‐liquid phase separation and review approaches for the fabrication of cell‐sized membranous protocells (e. g., giant unilamellar vesicles, polymersomes and proteinosomes). Three strategies are emphasized for the construction of advanced cell‐like structures consisting of liquid‐like subcompartments enclosed by a membrane: (i) adsorption and assembly of organic or inorganic species onto the surface of preformed coacervate microdroplets; (ii) interfacial assembly of a lipid or polymer bilayer on the surface of an aqueous pool containing preformed microdroplets; and (ii) triggering phase separation within a preformed semipermeable membrane with chemical or physical stimuli. This review underscores the significance of the hierarchical organization of these synthetic cellular structures in mimicking cytomimetic functions, including transmembrane signaling, protocell communication, prototissue formation, and their compelling biomedical applications.
Association between total hypercalcaemia and iliosacral lymph node metastasis in dogs diagnosed with anal sac adenocarcinoma using abdominal ultrasonography
Authors: Toth Darby , Upchurch David , Hallman R. Mackenzie 
Published: November 2023

AbstractBackgroundAnal sac adenocarcinoma (ASACA) in dogs is a malignant perianal tumour that often metastasizes to the iliosacral lymph nodes. Additionally, this tumour can be associated with hypercalcemia of malignancy. To date, no study has looked at the association between increased blood calcium levels and suspected or confirmed lymph node metastasis as a primary objective.ObjectiveThe objective of this study was to determine if increased total serum calcium level is associated with iliosacral lymph node metastasis in dogs diagnosed with ASACA.MethodsMedical records of a single referral hospital were searched to identify dogs examined between 2011 and 2021 that had a diagnosis of ASACA via cytology or histopathology. Only dogs that had serum total calcium recorded and abdominal ultrasound were included in the study. All images were reviewed by a board‐certified radiologist blinded to any patient identifiers.ResultsOf the 58 dogs, 33% (19/58) had total hypercalcaemia, and of these, 68% had confirmed or suspected iliosacral lymph node metastasis. Total hypercalcaemia was significantly associated with confirmed or suspected iliosacral lymph node metastasis (p < 0.01). However, 46% (11/24) of dogs with confirmed or suspected iliosacral lymph node metastasis were normocalcaemic.ConclusionsBased on these results, it is suggested that while the presence of total hypercalcaemia may increase the likelihood of concurrent lymph node metastasis, total hypercalcaemia alone cannot be used as a screening tool for lymph node metastasis. Dogs diagnosed with ASACA should undergo full staging regardless of total serum calcium values.
Prevalence and antimicrobial resistance of Campylobacter species in shelter‐housed healthy and diarrheic cats and dogs in Turkey
Authors: Yildiz Mustafa , Sahin Orhan , Adiguzel Mehmet Cemal 
Published: November 2023

AbstractBackgroundCampylobacter spp. are among the leading foodborne bacterial pathogens. Pet animals may be an important reservoir for human infection.ObjectivesTo determine the prevalence and antimicrobial resistance profiles and mechanisms of Campylobacter isolates recovered from shelter‐housed healthy and diarrheic cats and dogs in Erzurum province in Turkey.MethodsA total of 250 rectal swabs (from 124 cats and 126 dogs) collected between 2020 and 2021 were included in this study. The samples were cultured using a Campylobacter‐selective agar medium. A single suspect colony from each plate was purified and species identification was performed by PCR. Minimum inhibitory concentration (MIC) values were determined against eight antibiotics. Specific antimicrobial resistance genes (tetO and aphA‐3) and mutations (in gyrA) were screened by PCR and/or sequencing.ResultsA total of 26 (10.4%) isolates (25 Campylobacter jejuni and 1 Campylobacter coli) were obtained from the dogs; no Campylobacter was isolated from the cats. Of the C. jejuni isolates, 19.2% were resistant to nalidixic acid, 7.7% to ciprofloxacin and 3.8% to tetracycline and gentamicin per the CLSI interpretative criteria. The C. coli isolate was susceptible to all of the tested antibiotics. Thr‐86‐Ile mutation was the most common change detected in the gyrA gene in the quinolone‐resistant isolates.ConclusionWhile geographic and population differences exist, Campylobacter carriage and associated antibiotic resistance in dogs is common, emphasising the need for continuous surveillance in this species, particularly given its zoonotic potential.
Third order QCD predictions for fiducial W-boson production
Authors: Campbell John , Neumann Tobias 
Published: November 2023

Abstract
Measurements of W-boson production at the LHC have reached percent-level precision and impose challenging demands on theoretical predictions. Such predictions directly limit the precision of measurements of fundamental quantities such as the W-boson mass and the weak mixing angle. A dominant source of uncertainty in predictions is from higher-order QCD effects. We present a calculation of W-boson production at the level of $$ {\alpha}_s^3 $$

α
s
3

at fixed order and including transverse-momentum resummation. We further show predictions for a direct comparison with low-pileup ATLAS transverse-momentum and fiducial cross-section measurements at $$ \sqrt{s} $$

s

= 5.02 TeV. We discuss in detail the impact of modern PDFs. Our calculation including the matching to W+jet production at NNLO will be publicly available the upcoming CuTe-MCFM release and allows for theory-data comparison at the state-of-the-art level.
Holographic neutrino transport in dense strongly-coupled matter
Authors: Järvinen M. , Kiritsis E. , Nitti F. , Préau E. 
Published: November 2023

Abstract
A (toy) model for cold and luke-warm strongly-coupled nuclear matter at finite baryon density, is used to study neutrino transport. The complete charged current two-point correlators are computed in the strongly-coupled medium and their impact on neutrino transport is analyzed. The full result is compared with various approximations for the current correlators and the distributions, including the degenerate approximation, the hydrodynamic approximation as well as the diffusive approximation and we comment on their successes. Further improvements are discussed.
Development of a rapid heat cycle injection molding system using infrared radiation and convection heating and influence on morphology and mechanical properties
Authors: Simoes Ricardo , Macedo Cláudia , Laranjeira Jorge , Brito António M. , Simões Carla L. , Faria Luís 
Published: November 2023

AbstractConventional injection molding is widely used to produce plastic parts, mainly in the automotive industry, due to the high production ratios and quality of injection parts. Low mold temperatures are usually employed to decrease cycle molding time and final process costs; however, resulting surface defects include weld lines, sink marks, and warpage. Therefore, plastic parts are often subjected to secondary processes to reduce the surface defects. A dynamic mold heating and cooling control technology, rapid heat cycle molding (RHCM), was employed to optimize the injection molding process. The present study combined convection heating (pressurized water flow) and external infrared heating systems to investigate the effect of dynamic temperature control on the injection molding process. The infrared heating system was custom built to allow studying under controlled conditions the influence of several process parameters on the resulting morphology and mechanical properties. Results show significant gains from using the RHCM technology to optimize the conventional process, namely, at 100 °C no frozen layer is formed while simultaneously increasing the Young’s modulus. Industrial companies struggling with defects resulting from the thermal changes during injection molding can thus consider RHCM as a mitigation strategy and use these results as a guide for tool design and implementation of the technique.
Review-based method for evaluating key performance indicators: an application on warehouse system
Authors: Faveto Alberto , Traini Emiliano , Bruno Giulia , Chiabert Paolo 
Published: November 2023

AbstractThis paper presents a procedure for selecting, evaluating, and ranking the key performance indicators (KPIs) in the context of warehouse systems. The method aims to help warehouse managers identify and prioritize KPIs most relevant to their business objectives and performance goals, ultimately driving continuous improvement in warehouse performance. The paper provides three main scientific contributions: (i) a selection composed of 70 KPIs extracted from the analysis of 203 scientific articles and clustered according to their business and sustainability objectives, (ii) a KPI ranking based on four different metrics measured analyzing 585 scientific papers from Scopus and, finally, (iii) a fifth metric, measured trough a survey submitted to 15 industrial experts from as many different Italian companies, that is added to improve the ranking system. As a part of the method applied to a case study, the paper provides a comprehensive review of the current scientific literature and industrial sector state of the art on warehouse KPIs. This article proposes an overview of the most used KPIs in research and industry, a support for understanding formalizing definitions, and a guide on how they can be measured and used to manage warehouses.
Exploring capabilities of elemental mass spectrometry for determination of metal and biomolecules in extracellular vesicles
Authors: Martínez-García Jaime , Villa-Vázquez Alicia , Fernández Beatriz , González-Iglesias Héctor , Pereiro Rosario 
Published: November 2023

AbstractExtracellular vesicles (EVs) are increasingly recognized as crucial components influencing various pathophysiological processes, such as cellular homeostasis, cancer progression, and neurological disease. However, the lack of standardized methods for EV isolation and classification, coupled with ambiguity in biochemical markers associated with EV subtypes, remains a major challenge. This Trends article highlights the most common approaches for EV isolation and characterization, along with recent applications of elemental mass spectrometry (MS) to analyse metals and biomolecules in EVs obtained from biofluids or in vitro cellular models. Considering the promising capabilities of elemental MS, the article also looks ahead to the potential analysis of EVs at the single-vesicle and single-cell levels using ICP-MS. These approaches may offer valuable insights into individual characteristics of EVs and their functions, contributing to a deeper understanding of their role in various biological processes.
Graphical Abstract
Avian nuptial plumage coloration changes differently when exposed to urban and rural areas
Authors: Surmacki Adrian , Siefferman Lynn , Plackowska Anna 
Published: November 2023

Abstract
Avian plumage coloration could be altered by external factors like dust accumulation, but the effect of environmental conditions on such post-moult colour changes are poorly studied. Here, we investigated how exposure to the atmosphere in habitats of differing anthropogenic pressures modifies UV/blue structural plumage coloration — a sexually selected trait in eastern bluebirds Sialia sialis. We collected feather samples from live birds and distributed them in three types of habitats: (i) urban roads with low traffic, (ii) urban roads with high traffic, and (iii) rural roads with low traffic. After 4 weeks, the brightness of feathers decreased in all types of habitats-resulting in less-ornamented coloration. Changes in UV and blue chroma, however, varied with habitat type; chroma decreased in urban areas (becoming less ornamented), but increased in rural locations (becoming more ornamented). According to physiological models of avian colour vision, however, these changes in coloration tended to be below perceptual thresholds. Our results underscore the importance of how human activity can influence plumage colour of individual birds during the period between moult cycles which could have an impact on avian visual signalling. The study opens venues for further studies focusing on how the optical properties of specific airborne particles can influence coloration of wild-living animals.

Significance statement
Plumage coloration plays an important role in avian visual communication. In addition to moult-based change, coloration may change between moult cycles due to exposure to external factors like, for example, dust accumulation. Ecological factors modifying a passive plumage change, however, remains poorly studied. We collected samples of structurally coloured feather from a species wherein plumage colour is sexually selected and exposed them to outdoor conditions. We demonstrate that UV/blue chroma of coloration changes differently in urban and rural habitats. It increased in rural and decreased in urban areas. On the other hand, brightness decreased in both habitat types. Using physiological models of avian colour vision, however, we found that these colour changes were likely not perceptible to birds. Our study provides the first evidence that changes in coloration of fully grown feathers may be habitat dependent.
Musculoskeletal disorders and pain in agricultural workers in Low- and Middle-Income Countries: a systematic review and meta-analysis
Authors: Shivakumar Mrithula , Welsh Victoria , Bajpai Ram , Helliwell Toby , Mallen Christian , Robinson Michelle , Shepherd Thomas 
Published: November 2023

AbstractAgricultural workers constitute two-thirds of the population of Low- and Middle-Income Countries (LMIC) and are at increased risk of developing musculoskeletal disorders (MSD) due to high-risk activities. This systematic review and meta-analysis aim to synthesise the prevalence, predictors, and outcomes of musculoskeletal pain amongst agricultural workers to identify priority areas for prevention and development of early interventions. This systematic review and meta-analysis included Studies published from the inception of global electronic databases until 30 September 2022 were included. Prevalence estimates for MSDs among agricultural workers aged over 18 years in LMIC were extracted. Narrative synthesis summarized study findings and pooled estimates for 12-month pain prevalence were calculated. 7502 potential studies were identified. 64 studies (68,684 participants from 23 countries) were included in the systematic review; 33 studies were included in the meta-analysis. Low back pain was the most widely investigated symptom. The 12-month pooled prevalence of low back pain was highest in Africa [61.96% (45.69–76.22)] compared to Asia [54.16% (47.76–60.50)] and South/Central America [28.52%(10.91–50.33)]. Narrative synthesis found associations between MSDs, particular activities including heavy lifting and repetitive movements, and outcomes including reduced productivity. MSDs are common in agriculture workers in LMIC. Global prevalence of low back pain in farmers, particularly in Africa, is greater than in previously reported global prevalence in the general population. This may be attributed to environmental factors and high-risk activities which could be targeted for prevention and early intervention strategies to support individuals, prevent disability, and reduce loss of productivity.
Generation of human gastric assembloids from primary fetal organoids
Authors: Benedetti Giada , Jones Brendan C. , Sgualdino Francesca , De Coppi Paolo , Giobbe Giovanni Giuseppe 
Published: November 2023

Abstract
Purpose
Understanding human gastric epithelium homeostasis remains partial, motivating the exploration of innovative in vitro models. Recent literature showcases the potential of fetal stem cell-derived organoids in developmental and disease modelling and translational therapies. To scale the complexity of the model, we propose to generate assembloids, aiming to increase gastric maturation to provide new structural and functional insights.

Methods
Human fetal gastric organoids (fGOs) were expanded in 3D Matrigel cultures. Confluent organoid cultures were released from the Matrigel dome and resuspended in a collagen I hydrogel. Subsequently, the organoid mixture was seeded in a ring shape within a 24-well plate and allowed to gelate. The structure was lifted in the medium and cultured in floating conditions, allowing for organoid self-assembling into a gastric assembloid. After 10 days of maturation, the assembloids were characterized by immunostaining and RT-PCR, comparing different fetal developmental stages.

Results
Successful generation of human fetal gastric assembloids (fGAs) was achieved using spontaneous self-aggregation within the collagen I hydrogel. Immunostaining analysis of early and late fGAs showed the establishment of apico-basal cell polarity, secretion of gastric mucins, and the presence of chromogranin A in both samples. Transcriptional markers analysis revealed distinct disparities in markers associated with mature cell types between late and early fetal stages.

Conclusions
fGOs can reliably be generated from human fetal samples. This pioneering assembloid approach paves the way for advancing our comprehension of human gastric epithelium homeostasis and its perturbation, offering a better in vitro platform for the study of gastric epithelial development and therapeutic translation.
Basal osteotomy of the first metacarpal using patient-specific guides and instrumentation: biomechanical and 3D CT-based analysis
Authors: Nüesch Cléa , Schweizer Andreas , Weber Andreas , Reissner Lisa 
Published: November 2023

Abstract
Introduction
The aim of this study was to investigate the radiological outcomes of proximal closing metacarpal extension osteotomies using patient-specific guides and instruments (PSI) in early-stage trapeziometacarpal osteoarthritis to gain further insight into the joint loading surface and the benefits of the procedure.

Methods
In a prospective observational study, nine patients were included between 11/2020 and 12/2021, undergoing a total of ten proximal metacarpal extension osteotomies for basal thumb osteoarthritis. Computer-assisted surgical planning was performed using computed tomography (CT) and three-dimensional (3D) segmentation, allowing the fabrication of 3D-printed PSIs for surgical treatment. Inclusion criteria were a 1-year follow-up by CT to assess postoperative correction of the positional shift of the first metacarpal (MC1) and the location of peak loads compared with the preoperative situation.

Results
Radiographic analysis of the peak loading zone revealed a mean displacement on the articular surface of the trapezius of 0.4 mm ± 1.4 mm to radial and 0.1 mm ± 1.2 mm to palmar, and on the articular surface of the MC1 of 0.4 mm ± 1.4 mm to radial and 0.1 mm ± 1.2 mm to dorsal.

Conclusion
There were trends indicating that a flatter pressure distribution and a dorsal shift of the peak loading zone may contribute to an improvement in subjective pain and patient satisfaction associated with this surgical procedure. The non-significant radiological results and the minor dorsal-radial shifts in our small study group limit a firm conclusion.

Level of evidence
III.
Cerebrospinal fluid findings in patients with neurological manifestations in post-COVID-19 syndrome
Authors: Boesl Fabian , Goereci Yasemin , Gerhard Ameli , Bremer Benno , Raeder Vanessa , Schweitzer Finja , Hoppmann Uta , Behrens Janina , Bellmann-Strobl Judith , Paul Friedemann , Wildemann Brigitte , Jarius Sven , Prüss Harald , Audebert Heinrich J. , Warnke Clemens , Franke Christiana 
Published: November 2023

Abstract
Background
Information on cerebrospinal fluid (CSF) findings in patients with neurological manifestations in post-COVID-19 syndrome is scarce.

Methods
Retrospective evaluation of 84 CSF samples in patients fulfilling post-COVID-19 criteria in two neurological post-COVID-19 outpatient clinics.

Results
In 68% of samples, all CSF parameters were normal. The most frequent pathological CSF finding was elevation of total protein (median total protein 33.3 mg/dl [total range 18.5–116.2]) in 20 of 83 (24%) samples. The second most prevalent pathological finding was a blood–CSF barrier dysfunction as measured by elevation of QAlb (median QAlb 4.65 [2.4–13.2]) in 11/84 (13%). Pleocytosis was found in only 5/84 (6%) samples and was mild in all of them. CSF-restricted oligoclonal bands were found in 5/83 (6%) samples. Anti-neuronal autoantibodies in CSF were negative in most cases, whilst 12/68 (18%) samples were positive for anti-myelin autoantibodies in serum. PCR for herpesviridae (HSV-1/-2, VZV, EBV, CMV, HHV6) showed, if at all, only weakly positive results in CSF or EDTA whole blood/plasma.

Conclusions
The majority of samples did not show any pathologies. The most frequent findings were elevation of total protein and blood–CSF barrier dysfunction with no signs of intrathecal inflammation. CSF analysis still keeps its value for exclusion of differential diagnoses.