P-193. Risk Factors Associated with Patients Diagnosed with Clostridioides difficile Infection with no Documented Antibiotic Exposures
Authors: Pranjic Ana , Patel Ursula , Cheknis Adam K , Pacheco Susan M , Kociolek Larry K , Donskey Curtis , Cadnum Jennifer , Johnson Stuart , Samore Matthew H , Gerding Dale N , Evans Charlesnika T , Skinner Andrew M 
Published: January 2025

Abstract

Background
Clostridioides difficile infection (CDI) is a common healthcare-associated infection leading to significant morbidity and mortality. Antibiotic exposure is a primary risk factor associated with CDI, but other common risk factors for include advanced age, frequent hospitalization, immunosuppression, previous CDI, and gastric acid suppression. This study aimed to evaluate factors associated with CDI with no documented antibiotic exposure.

Methods
This is a case-control study of 131 patients with a positive C. difficile (CD) test between 7/2022 - 6/2023 at the Edward Hines Jr., VA Hospital. Incident cases were defined as a patient with a positive CD test with no documented antibiotic exposures within 120 days prior to diagnosis, and controls were patients with a positive CD test with documented antibiotic exposures. A multivariable logistic regression model was constructed to determine the adjusted odds ratio (aOR) and 95% confidence intervals (CI) for key CDI risk factors associated with primary CDI and no antibiotic exposure. Available stools were cultured, and recovered CD isolates underwent PCR-ribotyping (RT).

Results
Among all patients, 60% (78/131) had primary CDI, 20% (26/131) had recurrent CDI, and 20% (27/131) were presumed colonized. Among patients with primary CDI, 20% (16/78) had no documented antibiotic exposure. (Table 1) Unadjusted analysis revealed that patients with primary CDI and no antibiotic exposure had increased odds of 1) being age &lt; 65 (crude OR [cOR] 4.41; CI 1.41 – 14.50) 2) having a stool toxin EIA negative test (cOR 5.06; CI 1.27 – 34.00), 3) being classified as a community onset CDI (CO-CDI) (cOR 5.32; CI 1.63 – 20.90). After adjusting for potential confounding variables, patients had increased odds of being age &lt; 65 (aOR 3.52; CI 1.02 – 12.80) and being classified as a CO-CDI (aOR 4.58; CI 1.30 – 19.10). (Table 2) RT106 was the most common strain identified, but there was no strain type associated with a lack of antibiotic exposure.

Conclusion
Patients diagnosed with CDI and no antibiotic exposures appear to be younger, frequently toxin EIA negative, and are more likely to have a community-onset CDI. Further research is required to determine if there are atypical risk factors within the community that place these patients at risk of developing a CDI.

Disclosures
Larry K. Kociolek, MD, MSCI, Merck: Grant/Research Support Curtis Donskey, MD, Clorox: Grant/Research Support|Pfizer: Grant/Research Support Stuart Johnson, M.D., Acurx Pharmaceuticals: Advisor/Consultant|Bio-K Plus International: Advisor/Consultant|Ferring Phamraceuticals: Advisor/Consultant Dale N. Gerding, MD, AstraZeneca: Advisor/Consultant|Destiny Pharma: Advisor/Consultant|Destiny Pharma: Licensed IP to Destiny|Sebela: Advisor/Consultant|Sebela: Licensed IP Andrew M. Skinner, MD, BioK plus: Advisor/Consultant|Ferring Pharmaceuticals: Advisor/Consultant|Recursion pharmaceutical: Advisor/Consultant