bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Australian childhood obesity summit: the role of data and evidence in 'public' policy making
Authors: SA Nathan , E Develin , N Grove , AB Zwi 
Published: July 2005

Abstract

Background
Overweight and obesity in Australia has risen at an alarming rate over the last 20 years as in other industrialised countries around the world, yet the policy response, locally and globally, has been limited. Using a childhood obesity summit held in Australia in 2002 as a case study, this paper examines how evidence was used in setting the agenda, influencing the Summit debate and shaping the policy responses which emerged. The study used multiple methods of data collection including documentary analysis, key informant interviews, a focus group discussion and media analysis. The resulting data were content analysed to examine the types of evidence used in the Summit and how the state of the evidence base contributed to policy-making.

Results
Empirical research evidence concerning the magnitude of the problem was widely reported and largely uncontested in the media and in the Summit debates. In contrast, the evidence base for action was mostly opinion and ideas as empirical data was lacking. Opinions and ideas were generally found to be an acceptable basis for agreeing policy action coupled with thorough evaluation. However, the analysis revealed that the evidence was fiercely contested around food advertising to children and action agreed was therefore limited.

Conclusion
The Summit demonstrated that policy action will move forward in the absence of strong research evidence. Where powerful and competing groups contest possible policy options, however, the evidence base required for action needs to be substantial. As with tobacco control, obesity control efforts are likely to face ongoing challenges around the nature of the evidence and interventions proposed to tackle the problem. Overcoming the challenges in controlling obesity will be more likely if researchers and public health advocates enhance their understanding of the policy process, including the role different types of evidence can play in influencing public debate and policy decisions, the interests and tactics of the different stakeholders involved and the part that can be played by time-limited yet high profile events such as Summits.
Lithium alters brain activation in bipolar disorder in a task- and state-dependent manner: an fMRI study
Authors: Silverstone Peter H , Bell Emily C , Willson Morgan C , Dave Sanjay , Wilman Alan H 
Published: July 2005

Abstract

Background
It is unknown if medications used to treat bipolar disorder have effects on brain activation, and whether or not any such changes are mood-independent.

Methods
Patients with bipolar disorder who were depressed (n = 5) or euthymic (n = 5) were examined using fMRI before, and 14 days after, being started on lithium (as monotherapy in 6 of these patients). Patients were examined using a word generation task and verbal memory task, both of which have been shown to be sensitive to change in previous fMRI studies. Differences in blood oxygenated level dependent (BOLD) magnitude between the pre- and post-lithium results were determined in previously defined regions of interest. Severity of mood was determined by the Hamilton Depression Scale for Depression (HAM-D) and the Young mania rating scale (YMRS).

Results
The mean HAM-D score at baseline in the depressed group was 15.4 ± 0.7, and after 2 weeks of lithium it was 11.0 ± 2.6. In the euthymic group it was 7.6 ± 1.4 and 3.2 ± 1.3 respectively. At baseline mean BOLD signal magnitude in the regions of interest for the euthymic and depressed patients were similar in both the word generation task (1.56 ± 0.10 and 1.49 ± 0.10 respectively) and working memory task (1.02 ± 0.04 and 1.12 ± 0.06 respectively). However, after lithium the mean BOLD signal decreased significantly in the euthymic group in the word generation task only (1.56 ± 0.10 to 1.00 ± 0.07, p < 0.001). Post-hoc analysis showed that these differences were statistically significant in Broca's area, the left pre-central gyrus, and the supplemental motor area.

Conclusion
This is the first study to examine the effects of lithium on brain activation in bipolar patients. The results suggest that lithium has an effect on euthymic patients very similar to that seen in healthy volunteers. The same effects are not seen in depressed bipolar patients, although it is uncertain if this lack of change is linked to the lack of major improvements in mood in this group of patients. In conclusion, this study suggests that lithium may have effects on brain activation that are task- and state-dependent. Given the small study size and the mildness of the patient's depression these results require replication.
Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro
Authors: Geutskens S B , Otonkoski T , Pulkkinen M-A , Drexhage H A , Leenen P J M 
Published: July 2005

AbstractMacrophages are a heterogeneous population of cells that belong to the mononuclear phagocyte system. They play an important role in tissue homeostasis and remodeling and are also potent immune regulators. Pancreatic macrophages are critically involved in the development and pathogenesis of autoimmune diabetes. To elucidate the ontogeny of pancreatic macrophages, we characterized in this study the macrophages present in the adult and developing fetal pancreas of normal mice. We additionally examined the presence of local macrophage precursors and the involvement of macrophages in the growth of endocrine tissue in the fetal pancreas. We identified two phenotypically distinct macrophage subsets in the adult pancreas. The majority of macrophages was CD45+ER-MP23+MOMA-1+. Under noninflammatory conditions, only a minority (∼5%) of the pancreatic macrophages additionally expressed the macrophage marker F4/80. In contrast, in the fetal pancreas, phenotypically, mature macrophages were identified exclusively by their expression of F4/80 and lacked detectable staining with ER-MP23 and MOMA-1 antibodies. In fetal pancreas organ cultures, we could show that macrophages develop from pre-existing precursors, which are present in the fetal pancreas at embryonic age 12.5. Moreover, the number of macrophages increased significantly when macrophage-colony stimulating factor was added to these cultures. It is important that this increase of F4/80-positive cells was paralleled by an increase in the number of insulin-producing cells, suggesting that macrophages support the growth of these endocrine cells.
A novel role for Notch ligand Delta-1 as a regulator of human Langerhans cell development from blood monocytes
Authors: Hoshino Natsuki , Katayama Naoyuki , Shibasaki Tetsunori , Ohishi Kohshi , Nishioka Junji , Masuya Masahiro , Miyahara Yoshihiro , Hayashida Masahiko , Shimomura Daiki , Kato Takuma , Nakatani Kaname , Nishii Kazuhiro , Kuribayashi Kagemasa , Nobori Tsutomu , Shiku Hiroshi 
Published: July 2005

AbstractHuman Langerhans cells (LCs) are of hematopoietic origin, but cytokine regulation of their development is not fully understood. Notch ligand Delta-1 is expressed in a proportion of the skin. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and transforming growth factor-β1 (TGF-β1) are also secreted in the skin. We report here that Delta-1, in concert with GM-CSF and TGF-β1, induces the differentiation of human CD14+ blood monocytes into cells that express LC markers: CD1a, Langerin, cutaneous lymphocyte-associated antigen, CC chemokine receptor 6, E-cadherin, and Birbeck granules. The resulting cells display phagocytic activity and chemotaxis to macrophage inflammatory protein-1α (MIP-1α). In response to CD40 ligand and tumor necrosis factor α, the cells acquire a mature phenotype of dendritic cells that is characterized by up-regulation of human leukocyte antigen (HLA)-ABC, HLA-DR, CD80, CD86, CD40, and CD54 and appearance of CD83. These cells in turn show chemotaxis toward MIP-1β and elicit activation of CD8+ T cells and T helper cell type 1 polarization of CD4+ T cells. Thus, blood monocytes can give rise to LCs upon exposure to the skin cytokine environment consisting of Delta-1, GM-CSF, and TGF-β1, which may be, in part, relevant to the development of human epidermal LCs. Our results extend the functional scope of Notch ligand δ-1 in human hematopoiesis.
Serum Parathyroid Hormone Concentrations Are Increased in Women with Polycystic Ovary Syndrome
Authors: Panidis Dimitrios , Balaris Christos , Farmakiotis Dimitrios , Rousso David , Kourtis Anargyros , Balaris Vassilios , Katsikis Ilias , Zournatzi Vassiliki , Diamanti-Kandarakis Evanthia 
Published: July 2005

Abstract
Background: The present study was designed to investigate the effects of polycystic ovary syndrome (PCOS) and of obesity on serum parathyroid hormone (ΡΤΗ), 25-hydroxyvitamin D (25-OH-vitamin D), and 1,25-dihydroxyvitamin D [1,25-(OH)2-vitamin D] concentrations and the possible associations of the above calciotropic hormones with the hormonal and metabolic characteristics of the syndrome.
Methods: We studied 58 obese [body mass index (BMI) &gt;30 kg/m2] women with PCOS, 64 overweight (ΒΜI, 25–30 kg/m2) women with the syndrome, 169 normal-weight (BMI &lt;25 kg/m2) women with PCOS, 29 obese controls (ovulatory women without clinical or biochemical hyperandrogenemia), 14 overweight controls, and 70 normal-weight controls. Blood samples were collected (at 0900 after an overnight fast) between the 3rd and 6th days of a menstrual cycle in the control groups and during a spontaneous bleeding episode in the PCOS groups. Circulating concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin (PRL), testosterone, Δ4-androstenedione, 17α-hydroxyprogesterone, sex-hormone–binding globulin (SHBG), insulin, glucose, PTH, 25-OH-vitamin D, and 1,25-(OH)2-vitamin D were measured.
Results: Both PCOS and increased body weight had a significant positive effect on serum PTH values. PTH concentrations were significantly correlated with age, BMI, glucose, PRL, SHBG, and testosterone. Only the correlations with testosterone and PRL were BMI-independent. The effect of PCOS on PTH concentrations remained significant after adjustment for BMI, but not after adjustment for testosterone concentration. Increased body weight also had a significant negative effect on 25-OH- and 1,25-(OH)2-vitamin D concentrations, but no association with the syndrome was observed.
Conclusions: The results of the present study are in agreement with previous data supporting an association of increased PTH and decreased vitamin D metabolite concentrations with obesity. Moreover, the present findings indicate, for the first time, that PTH probably is also linked to PCOS-associated hyperandrogenism.
The Detection of UV‐induced Membrane Damages by a Combination of Two Biosensor Techniques
Authors: Christ Katrin , Rüttinger Hans‐Hermann , Höpfner Matthias , Rothe Ulrich , Bendas Gerd 
Published: July 2005

ABSTRACTKetoprofen is an important anti‐inflammatory drug, but its dermal application is limited because of the photosensitizing properties causing phototoxic reactions of the skin when exposed to UV light. We have recently investigated the peroxide formation of ketoprofen in solutions of linoleic acid during UV irradiation. To continue these studies and focus on UV‐induced changes in membrane integrity and barrier function we established an in vitro model using two biosensor techniques simultaneously. Support‐fixed bilayers were irradiated with different doses of UV‐B up to damaging intensities with or without ketoprofen. Cyclic voltammetry was carried out to detect alterations in membrane permeability; quartz crystal microbalance (QCM) measurements were helpful in analyzing whether a permeability increase was caused by depletion of membrane components. In absence of ketoprofen, increasing UV‐B doses induce membrane permeabilities of both unsaturated and saturated bilayers. QCM measurements could not reveal a significant loss of membrane components as a reason for the permeability. In contrast, 0.3 mM ketoprofen induced a dose‐dependent increase in membrane permeability. QCM results indicated a mass loss. Although this model does not explain all molecular mechanisms of membrane damage by ketoprofen, the combined application of both QCM and CV is a novel and powerful tool to investigate functional mechanisms of UV‐induced membrane damages.
Validation and Application of a Fish-Based Index of Biotic Integrity for Small Central Minnesota Lakes
Authors: Drake Melissa T. , Valley Ray D. 
Published: July 2005

Abstract
We evaluated the performance of an index of biotic integrity (IBI) based on 16 fish population metrics of three types: species richness, community assemblage, and trophic composition. Two sets of central Minnesota lakes independent from the original set of lakes used to develop the IBI model were used to validate it. One set of lakes (n = 15) had physical features similar to those used to develop the IBI, while the other set (n = 22) averaged 9 m shallower with 28% more littoral area. We used general linear models to test whether the relationships between IBI or individual metric score and indicators of lake quality (trophic state, floristic quality, or surrounding land use) were the same or differed for the original IBI data set and each new data set. Responses were similar among all data sets, lake IBI scores and individual metrics reflecting differences in land use, trophic state, and aquatic habitat. Sensitivity of individual metrics to different measures of stress varied, supporting the need for a multimetric approach when assessing the biotic integrity of lakes. Index of biotic integrity scores were most highly correlated with trophic state (rho = −0.80). Our results support the validity of the original fish-based IBI as a standardized method for quantitatively measuring the condition of fish assemblages and implied overall biotic integrity of small central Minnesota lakes. As with any model, however, continued evaluation is recommended, especially when applying this IBI to lakes with different physical, chemical, or biological characteristics.
Mathematical Existence
Authors: Maddy Penelope 
Published: July 2005

Despite some discomfort with this grandly philosophical topic, I do in fact hope to address a venerable pair of philosophical chestnuts: mathematical truth and existence. My plan is to set out three possible stands on these issues, for an exercise in compare and contrast. A word of warning, though, to philosophical purists (and perhaps of comfort to more mathematical readers): I will explore these philosophical positions with an eye to their interconnections with some concrete issues of set theoretic method.Let me begin with a brief look at what to count as ‘philosophy’. To some extent, this is a matter of usage, and mathematicians sometimes classify as ‘philosophical’ any considerations other than outright proofs. So, for example, discussions of the propriety of particular mathematical methods would fall under this heading: should we prefer analytic or synthetic approaches in geometry? Should elliptic functions be treated in terms of explicit representations (as in Weierstrass) or geometrically (as in Riemann)? Should we allow impredicative definitions? Should we restrict ourselves to a logic without bivalence or the law of the excluded middle? Also included in this category would be the trains of thought that shaped our central concepts: should a function always be defined by a formula? Should a group be required to have an inverse for every element? Should ideal divisors be defined contextually or explicitly, treated computationally or abstractly? In addition, there are more general questions concerning mathematical values, aims and goals: Should we strive for powerful theories or low-risk theories? How much stress should be placed on the fact or promise of physical applications? How important are interconnections between the various branches of mathematics? These philosophical questions of method naturally include several peculiar to set theory: should set theorists focus their efforts on drawing consequences for areas of interest to mathematicians outside mathematical logic? Should exploration of the standard axioms of ZFC be preferred to the exploration and exploitation of new axioms? How should axioms for set theory be chosen? What would a solution to the Continuum Problem look like?
The Role of True Finiteness in the Admissible Recursively Enumerable Degrees
Authors: Greenberg Noam 
Published: July 2005

AbstractWhen attempting to generalize recursion theory to admissible ordinals, it may seem as if all classical priority constructions can be lifted to any admissible ordinal satisfying a sufficiently strong fragment of the replacement scheme. We show, however, that this is not always the case. In fact, there are some constructions which make an essential use of the notion of finiteness which cannot be replaced by the generalized notion of α-finiteness. As examples we discuss both codings of models of arithmetic into the recursively enumerable degrees, and non-distributive lattice embeddings into these degrees. We show that if an admissible ordinal α is effectively close to ω (where this closeness can be measured by size or by cofinality) then such constructions may be performed in the α-r.e. degrees, but otherwise they fail. The results of these constructions can be expressed in the first-order language of partially ordered sets, and so these results also show that there are natural elementary differences between the structures of α-r.e. degrees for various classes of admissible ordinals α. Together with coding work which shows that for some α, the theory of the α-r.e. degrees is complicated, we get that for every admissible ordinal α, the α-r.e. degrees and the classical r.e. degrees are not elementarily equivalent.
Tree structures associated to a family of functions
Authors: Argyros Spiros A. , Dodos Pandelis , Kanellopoulos Vassilis 
Published: July 2005

The research presented in this paper was motivated by our aim to study a problem due to J. Bourgain [3]. The problem in question concerns the uniform boundedness of the classical separation rank of the elements of a separable compact set of the first Baire class. In the sequel we shall refer to these sets (separable or non-separable) as Rosenthal compacta and we shall denote by ∝(f) the separation rank of a real-valued functionfinB1(X), withXa Polish space. Notice that in [3], Bourgain has provided a positive answer to this problem in the case ofKsatisfyingwithXa compact metric space. The key ingredient in Bourgain's approach is that whenever a sequence of continuous functions pointwise converges to a functionf, then the possible discontinuities of the limit function reflect a local ℓ1-structure to the sequence (fn)n. More precisely the complexity of this ℓ1-structure increases as the complexity of the discontinuities offdoes. This fruitful idea was extensively studied by several authors (c.f. [5], [7], [8]) and for an exposition of the related results we refer to [1]. It is worth mentioning that A.S. Kechris and A. Louveau have invented the rank rND(f) which permits the link between thec0-structure of a sequence (fn)nof uniformly bounded continuous functions and the discontinuities of its pointwise limit. Rosenthal'sc0-theorem [11] and thec0-index theorem [2] are consequences of this interaction.Passing to the case where either (fn)nare not continuous orXis a non-compact Polish space, this nice interaction is completely lost.
A logic for metric and topology
Authors: Wolter Frank , Zakharyaschev Michael 
Published: July 2005

AbstractWe propose a logic for reasoning about metric spaces with the induced topologies. It combines the ‘qualitative’ interior and closure operators with ‘quantitative’ operators ‘somewhere in the sphere of radiusr’ including or excluding the boundary. We supply the logic with both the intended metric space semantics and a natural relational semantics, and show that the latter (i) provides finite partial representations of (in general) infinite metric models and (ii) reduces the standard ‘ε-definitions’ of closure and interior to simple constraints on relations. These features of the relational semantics suggest a finite axiomatisation of the logic and provide means to prove its EXPTIME-completeness (even if the rational numerical parameters are coded in binary). An extension with metric variables satisfying linear rational (in)equalities is proved to be decidable as well. Our logic can be regarded as a ‘well-behaved’ common denominator of logical systems constructed in temporal, spatial, and similarity-based quantitative and qualitative representation and reasoning. Interpreted on the real line (with its Euclidean metric), it is a natural fragment of decidable temporal logics for specification and verification of real-time systems. On the real plane, it is closely related to quantitative and qualitative formalisms for spatial representation and reasoning, but this time the logic becomes undecidable.
Preserving preservation
Authors: Kellner Jakob , Shelah Saharon 
Published: July 2005

AbstractWe prove that the property “P doesn't make the old reals Lebesgue null” is preserved under countable support iterations of proper forcings, under the additional assumption that the forcings are nep (a generalization of Suslin proper) in an absolute way. We also give some results for general Suslin ccc ideals.
The axiom of elementary sets on the edge of Peircean expressibility
Authors: Formisano Andrea , Omodeo Eugenio G. , Policriti Alberto 
Published: July 2005

AbstractBeing able to state the principles which lie deepest in the foundations of mathematics by sentences in three variables is crucially important for a satisfactory equational rendering of set theories along the lines proposed by Alfred Tarski and Steven Givant in their monograph of 1987.The main achievement of this paper is the proof that the ‘kernel’ set theory whose postulates are extensionality. (E), and single-element adjunction and removal. (W) and (L), cannot be axiomatized by means of three-variable sentences. This highlights a sharp edge to be crossed in order to attain an ‘algebraization’ of Set Theory. Indeed, one easily shows that the theory which results from the said kernel by addition of the null set axiom, (N), is in its entirety expressible in three variables.
PNA Encoding (PNA=peptide nucleic acid): From Solution‐Based Libraries to Organized Microarrays
Authors: Harris Jennifer L. , Winssinger Nicolas 
Published: July 2005

AbstractMicroarray‐based technologies have attracted attention in chemical biology by virtue of their miniaturized format, which is well suited to probe ligand–protein interactions or investigate enzymatic activity in complex biological mixtures. A number of research groups have reported the preparation of surfaces on microarrays with specific functional groups to chemoselectively attach small molecules from libraries. We have developed an alternative method whereby libraries are encoded with peptide nucleic acid (PNA), such that libraries which exist as mixtures in solution self‐assemble into an organized microarray through hybridization to produce readily available DNA arrays. This allows libraries synthesized by split and mix methods to be decoded in a single step. An asset of this method compared to direct spotting is that libraries can be used in solution for bioassays prior to self‐assembly into the microarray format.