bluuebunny/crossref_metadata_2025_split huggingface dataset:
Percutaneous calcium sulfate injection versus localized scrape bone grafting: clinical effect comparison in titanium elastic nail treatment of pathological fracture of proximal humerus caused by unicameral bone cysts in children
Authors: Xie Yuyin , Song Zhenqi , Tang Zhongwen , Xu Zheng , Rao Zhouzhou , Wen Jie , Xiao Sheng 
Published: January 2024

ObjectiveA retrospective study was conducted to compare the mid-term clinical efficacy between percutaneous calcium sulfate injection (PCSI) and localized scrape bone grafting (LSBG) in using titanium elastic nails treat humerus pathologic fractures caused by unicameral bone cysts in children.MethodsHumerus pathologic fracture patients with unicameral bone cysts in our pediatric orthopedic department from January 2015 to January 2020 were retrospectively analyzed. Patients were divided into two groups, namely the PCSI group and the LSBG group, based on the type of bone grafting material they received. Preoperative assessments were made in both groups using the Cyst Index and Cyst activity. During the perioperative phase, assessments were made regarding operative time, bleeding, postoperative VAS scores, and the frequency of reoperation within 2 years. Clinical outcomes were evaluated using the Capanna scale at the last follow-up, and the occurrence of re-fractures during the follow-up period.ResultsA total of 22 patients were included, with a mean follow-up duration of 33.5 ± 5.8 months. No significant differences were found between the two groups in terms of Cyst Index and Cyst activity before the operation. The operative time, bleeding, and postoperative VAS scores in the PCSI group were found to be lower than those in the LSBG group (P &lt; 0.05). The PCSI group also showed a higher frequency of reoperation within 2 years compared to the LSBG group (P &lt; 0.05). However, no significant differences were observed between the two groups in terms of Capanna scale scores at the last follow-up and the incidence of re-fractures during follow-up.ConclusionsBoth titanium elastic nails (TEN) combined with PCSI or LSBG were found to be safe and effective treatments for humerus pathologic fractures caused by unicameral bone cysts in children. PCSI is considered as a less invasive option with shorter operative times, less bleeding, and reduced postoperative pain, although it comes with the risk of multiple injections. On the other hand, LSBG is considered as a more invasive option for the treatment of active bone cysts but is associated with a lower recurrence rate.
Pharmacokinetics, metabolite profiling, safety and tolerability of YZJ-4729 tartrate, a novel G protein-biased μ-opioid receptor agonist, in healthy Chinese subjects
Authors: Ni Yufeng , Gao Huaye , Ouyang Wen , Yang Guoping , Cheng Minlu , Ding Li 
Published: January 2024

Objective:YZJ-4729 is a novel G protein-biased μ-opioid receptor agonist for the treatment of acute pain in adult patients who require intravenous opioid analgesic therapy. The aim of this study was to assess the pharmacokinetics, metabolite profiling, safety and tolerability of YZJ-4729 in healthy Chinese subjects following the single intravenous doses ranged from 0.2 mg to 6 mg.Methods:This single-center, randomized, double-blind, placebo-controlled clinical study was conducted in 54 healthy male and female Chinese subjects after single ascending doses of YZJ-4729 tartrate (0.2, 0.5, 1.5, 3, 4.5, and 6 mg). Subjects in each cohort were assigned randomly to receive a single intravenous dose of YZJ-4729 tartrate injection or placebo at a ratio of 4:1. Pharmacokinetic characteristics, metabolite profiling, safety and tolerability profiles of the study drug were evaluated.Results:Overall, YZJ-4729 was safe and well tolerated in healthy Chinese subjects. The study drug reached peak plasma concentrations nearly at the end of the infusion. After administration, YZJ-4729 was eliminated rapidly with a terminal elimination half-life of 0.862–2.50 h, and excreted little in human excreta. The maximum drug concentration and area under the plasma concentration-time curve increased with dose escalation across the entire dose range. YZJ-4729 experienced extensive metabolism in human body. A total of 19 metabolites were identified and the characteristic metabolic pathways involved hydroxylation, ketone formation, N-dealkylation and glucuronide conjugation. Metabolite M10 was the most abundant circulating metabolite, and represented over 10% of total drug-related systemic exposure. Further PK and safety evaluation of M10 was necessary.Conclusion:The clinical study results laid a foundation for the further clinical studies of YZJ-4729 in patients.Clinical Trial Registration:http://www.chinadrugtrials.org.cn, identifier CTR20222574.
Regulation of feeding dynamics by the circadian clock, light and sex in an adult nocturnal insect
Authors: Force Evan , Sokolowski Michel B. C. , Suray Caroline , Debernard Stéphane , Chatterjee Abhishek , Dacher Matthieu 
Published: January 2024

Animals invest crucial resources in foraging to support development, sustenance, and reproduction. Foraging and feeding behaviors are rhythmically expressed by most insects. Rhythmic behaviors are modified by exogenous factors like temperature and photoperiod, and internal factors such as the physiological status of the individual. However, the interactions between these factors and the circadian clock to pattern feeding behavior remains elusive. As Drosophila, a standard insect model, spends nearly all its life on food, we rather chose to focus on the adults of a non-model insect, Agrotis ipsilon, a nocturnal cosmopolitan crop pest moth having structured feeding activity. Our study aimed to explore the impact of environmental cues on directly measured feeding behavior rhythms. We took advantage of a new experimental set-up, mimicking an artificial flower, allowing us to specifically monitor feeding behavior in a naturalistic setting, e.g., the need to enter a flower to get food. We show that the frequency of flower visits is under the control of the circadian clock in males and females. Feeding behavior occurs only during the scotophase, informed by internal clock status and external photic input, and females start to visit flowers earlier than males. Shorter duration visits predominate as the night progresses. Importantly, food availability reorganizes the microstructure of feeding behavior, revealing its plasticity. Interestingly, males show a constant number of daily visits during the 5 days of adult life whereas females decrease visitations after the third day of adult life. Taken together, our results provide evidence that the rhythmicity of feeding behavior is sexually dimorphic and controlled by photoperiodic conditions through circadian clock-dependent and independent pathways. In addition, the use of the new experimental set-up provides future opportunities to examine the regulatory mechanisms of feeding behavior paving the way to investigate complex relationships between feeding, mating, and sleep-wake rhythms in insects.
Assessment of inter-individual variability in hamstring muscle recovery after a sport-specific sprint training in women and men
Authors: Cosio Pedro L. , Moreno-Simonet Lia , Porcelli Aniello , Lloret Mario , Padulles Xavier , Padulles Josep M. , Farran-Codina Andreu , Cadefau Joan A. 
Published: January 2024

Background: Hamstring muscles are most affected by multiple sprint-based sports as a result of muscle strain during sprinting, leading to reduced performance and increased risk of injury. Therefore, the purpose of the study was to assess inter-individual variability in hamstrings recovery after a sport-specific repeated-sprint training (RST), through sprint-specific markers of muscle recovery and associated muscle damage biomarkers in women and men.Methods: Healthy females (n = 14) and males (n = 15) underwent 10 repeated 40-m sprints with a 3-min rest pause between each repetition. Force-generating capacity (FGC) by the 90°hip:20°kneetest and range of motion Jurdan test, together with serum biomarkers [sarcomeric mitochondrial creatine kinase (sMtCK), oxidative stress, irisin] were tested at baseline and 24-, 48- and 72-h post-exercise through a repeated measures design. Participants were classified according to FGC loss into high responders (HR) and low responders (LR).Results: 21 individuals (10 females, 11 males) were classified as HR (FGC loss &gt;20% and recovery &gt;48 h), while 8 individuals (4 females, 4 males) were classified as LR. HR individuals showed unrecovered maximal voluntary isometric contraction (MVIC) torque until 72 h post-training (p = 0.003, np2 = 0.170), whereas only HR males showed decreased range of motion (p = 0.026, np2 = 0.116). HR individuals also showed increased sMtCK (p = 0.016, np2 = 0.128), oxidative stress (p = 0.038, np2 = 0.106) and irisin (p = 0.019, np2 = 0.123).Conclusion: There is inter-individual variability in the muscular response to a sport-specific RST, identifiable by MVIC torque assessment. The findings support that the 90°hip:20°kneetest is a powerful indirect test to screen hamstrings recovery in both women and men, in a cost-effective way. However, the Jurdan test might not be able to monitor hamstrings recovery in sportswomen after RST. Decreases in muscle capacity are linked to damage to muscle sarcolemma and mitochondria until 72 h post-exercise. Overall, 72 h will not be adequate time to restore hamstrings structure and function after a sport-specific RST in both female and male responders.
Worldwide productivity and research trend on fruit quality: a bibliometric study
Authors: Ni Fei , Li Ziwei , Huang Jianzi 
Published: January 2024

IntroductionAs one of the important sources of food for human beings, fruits have been extensively studied. To better guide basic and applied research, it is urgent to conduct a systematic analysis of these studies based on extensive literature collection.MethodsBased on the Web of Science Core Collection database, this study uses R language and CiteSpace to conduct bibliometric analysis and data mining on the literatures related to fruit quality from January 2013 to June 2023.ResultsThe results indicated that among various fruits, tomatoes have been most frequently studied with special interests in photosynthesis, fruit development, and molecular breeding. The research direction primarily focused on fruit resistance and storage characteristics. Among the indicators related to fruit quality, antioxidant activity has the highest co-occurrence with other indicators of fruit quality, especially with nutrients such as anthocyanins, phenolic substances, sugars, and fruit firmness.DiscussionCurrently, adaptation to stress and antioxidant activity are recognized as prominent research focal points in this field. Fruit morphology, particularly fruit size, irrigation methods, application of molecular technology, and infection prevention, represent potential areas of interests in future research on fruit quality.
A genetic screen for dominant chloroplast reactive oxygen species signaling mutants reveals life stage-specific singlet oxygen signaling networks
Authors: Lemke Matthew D. , Woodson Jesse D. 
Published: January 2024

IntroductionPlants employ intricate molecular mechanisms to respond to abiotic stresses, which often lead to the accumulation of reactive oxygen species (ROS) within organelles such as chloroplasts. Such ROS can produce stress signals that regulate cellular response mechanisms. One ROS, singlet oxygen (1O2), is predominantly produced in the chloroplast during photosynthesis and can trigger chloroplast degradation, programmed cell death (PCD), and retrograde (organelle-to-nucleus) signaling. However, little is known about the molecular mechanisms involved in these signaling pathways or how many different signaling 1O2 pathways may exist.MethodsThe Arabidopsis thaliana plastid ferrochelatase two (fc2) mutant conditionally accumulates chloroplast 1O2, making fc2 a valuable genetic system for studying chloroplast 1O2-initiated signaling. Here, we have used activation tagging in a new forward genetic screen to identify eight dominant fc2 activation-tagged (fas) mutations that suppress chloroplast 1O2-initiated PCD.ResultsWhile 1O2-triggered PCD is blocked in all fc2 fas mutants in the adult stage, such cellular degradation in the seedling stage is blocked in only two mutants. This differential blocking of PCD suggests that life-stage-specific 1O2-response pathways exist. In addition to PCD, fas mutations generally reduce 1O2-induced retrograde signals. Furthermore, fas mutants have enhanced tolerance to excess light, a natural mechanism to produce chloroplast 1O2. However, general abiotic stress tolerance was only observed in one fc2 fas mutant (fc2 fas2). Together, this suggests that plants can employ general stress tolerance mechanisms to overcome 1O2 production but that this screen was mostly specific to 1O2 signaling. We also observed that salicylic acid (SA) and jasmonate (JA) stress hormone response marker genes were induced in 1O2-stressed fc2 and generally reduced by fas mutations, suggesting that SA and JA signaling is correlated with active 1O2 signaling and PCD.DiscussionTogether, this work highlights the complexity of 1O2 signaling by demonstrating that multiple pathways may exist and introduces a suite of new 1O2 signaling mutants to investigate the mechanisms controlling chloroplast-initiated degradation, PCD, and retrograde signaling.
Differences in training characteristics of recreational endurance runners by race distance – results from the NURMI Study (Step 2)
Authors: Knechtle Beat , Tanous Derrick , Thuany Mabliny , Motevalli Mohamad , Wirnitzer Gerold , Leitzmann Claus , Weiss Katja , Rosemann Thomas , Wirnitzer Katharina 
Published: January 2024

BackgroundAlthough runner’s profiles were previously investigated, information on the training frequency and training distance for short (5 km, 10 km) and long-distance (&gt;21 km) running is absent. The present study aimed to investigate the associations between training routines and exercise habits of recreational endurance runners considering self-reported preferred race distance [10 km, half-marathon (HM), and marathon/ultra-marathon (M/UM)] subgroups.MethodsThis is a cross-sectional study, sampling 154 recreational runners of both sexes. A web survey was used for data collection regarding age, sex, preferred distance (10-km, HM, M/UM), training routines, exercise habits, and periodized training routines. The Chi-square test (Cramer’s V) and Kruskal-Wallis test (Eta-Squared η2) with effect sizes were used for comparisons between race distances.ResultsSignificant differences were shown for anthropometric, training, and periodization characteristics. Highly significant differences were found between subgroups for the number of sessions, running kilometers, and training hours at all periods and within all four preparation conditions. M/UM runners were training more frequently, for longer durations, and ran greater distances each week.ConclusionThis finding supports the notion that training habits and periodization characteristics are different for different race distances (10-km, half marathon, marathon, and ultramarathon).
Helicopter parenting and college student depression: the mediating effect of physical self-esteem
Authors: Wang Chaolian , Shi Heng , Li Geng 
Published: January 2024

BackgroundDepression is one of the most common and prevalent mental disorders, and college students are a high-risk group for depression. Helicopter parenting plays an important role in depression, but the mechanism is still ambiguous. Therefore, this study investigates the specific impact and mechanism of helicopter parenting on college students’ depression.MethodsEmploying a questionnaire-based approach, we assessed the relationship between helicopter parenting, Physical self-esteem, and depression. The questionnaire comprised three scales: the Helicopter Parenting Scale, Physical Self-Esteem Scale, and Self-Rating Depression Scale. The study sample included 539 university (average age 18.84 ± 1.1 years; 184 males and 355 females).ResultsHelicopter parenting demonstrated significant negative predict with physical self-esteem (β = −0.75, p &lt; 0.001), and positive predict depression (β = 0.33, p &lt; 0.001). Helicopter parenting impacts depression among college students through two channels: solely via physical self-esteem (mediating effect value: 0.66), and through direct influence (effect value: 0.64).ConclusionThe insights from this study address the two pivotal questions about “why” and “how” helicopter parenting influences depression in college students, offering recommendations for managing depressive moods among college students.
Many hops, many stops: care-seeking “loops” for diabetes and hypertension in three urban informal settlements in the Mumbai Metropolitan Region
Authors: Ramani Sudha , Bahuguna Manjula , Spencer Jennifer , Pathak Sweety , Shende Sushma , Pantvaidya Shanti , D’Souza Vanessa , Jayaraman Anuja 
Published: January 2024

BackgroundThe burden of Non-Communicable Diseases (NCDs) in urban informal settlements across Lower and Middle Income Countries is increasing. In recognition, there has been interest in fine-tuning policies on NCDs to meet the unique needs of people living in these settlements. To inform such policy efforts, we studied the care-seeking journeys of people living in urban informal settlements for two NCDs—diabetes and hypertension. The study was done in the Mumbai Metropolitan Region, India.MethodsThis qualitative study was based on interviews with patients having diabetes and hypertension, supplemented by interactions with the general community, private doctors, and public sector staff. We conducted a total of 47 interviews and 6 Focus Group Discussions. We synthesized data thematically and used the qualitative software NVivo Version 10.3 to aid the process. In this paper, we report on themes that we, as a team, interpreted as striking and policy-relevant features of peoples’ journeys.ResultsPeople recounted having long and convoluted care-seeking journeys for the two NCDs we studied. There were several delays in diagnosis and treatment initiation. Most people’s first point of contact for medical care were local physicians with a non-allopathic degree, who were not always able to diagnose the two NCDs. People reported seeking care from a multitude of healthcare providers (public and private), and repeatedly switched providers. Their stories often comprised multiple points of diagnosis, re-diagnosis, treatment initiation, and treatment adjustments. Advice from neighbors, friends, and family played an essential role in shaping the care-seeking process. Trade-offs between saving costs and obtaining relief from symptoms were made constantly.ConclusionOur paper attempts to bring the voices of people to the forefront of policies on NCDs. People’s convoluted journeys with numerous switches between providers indicate the need for trusted “first-contact” points for NCD care. Integrating care across providers—public and private—in urban informal settlements—can go a long way in streamlining the NCD care-seeking process and making care more affordable for people. Educating the community on NCD prevention, screening, and treatment adherence; and establishing local support mechanisms (such as patient groups) may also help optimize people’s care-seeking pathways.
Scaling up evidence-based digital early life nutrition interventions in a county setting: an implementation trial – protocol for Phase 2 of the Nutrition Now project
Authors: Medin Anine Christine , Vik Frøydis Nordgård , Helle Christine , Helland Sissel Heidi , Wills Andrew Keith , Osorio Natalie Garzon , Lian Henrik , Ersfjord Torunn Iveland , Van Daele Wim , Bjørkkjær Tormod , Valen Erlend Nuland , Gebremariam Mekdes Kebede , Grasaas Erik , Kiland Charlotte , von Thiele Schwarz Ulrica , Abel Marianne Hope , Love Penny , Campbell Karen , Rutter Harry , Barker Mary Elizabeth , Hillesund Elisabet Rudjord , Øverby Nina Cecilie 
Published: January 2024

BackgroundFew effective health interventions transition from smaller efficacy or effectiveness studies to real-world implementation at scale, representing a gap between evidence and practice. Recognising this, we have developed Nutrition Now – a tailored digital resource building on four efficacious dietary interventions, aiming to improve nutrition in the important first 1,000 days of life. Nutrition Now targets and guides expectant parents and parents of 0–2 year olds, serves as a reliable source of evidence-based information for midwives and public health nurses at maternal and child healthcare (MCH) centres, and offers pedagogical tools for early childhood education and care (ECEC) staff. The aim of this study is to implement Nutrition Now at scale and evaluate the impact of different sets of multifaceted implementation strategies on implementation outcomes.MethodsA quasi-experimental design with three study arms will be used, providing either low, medium or high implementation support, when rolled out in 50 municipalities in 2 counties in Norway. Nutrition Now will be implemented in MCH and ECEC settings and made available to expectant parents and parents of 0–2 year olds through social media and MCH. The implementation support builds on strategies described in the Expert Recommendations for Implementing Change (ERIC) implementation framework and is informed by dialogues with stakeholders. Impact of the different degree of implementation support will be assessed by examining reach, adoption, fidelity, and sustainability using usage data generated from the Nutrition Now resource, publicly available municipal data and qualitative interviews with MCH and ECEC staff.DiscussionNutrition Now Phase 2 will break new ground by scaling up successively delivered and complementary dietary interventions in the first 1,000 days of life in a real-life context. The project also seeks to identify what level of implementation support is most effective when implementing digital, scalable, evidence-based early-life nutrition interventions in community settings. The project will inform implementation research and provide knowledge about effective implementation strategies to be used in a national scale-up of Nutrition Now.Trial registrationThe study is registered prospectively (submitted 14/06/2022, registration date: 19/06/2022) in the International Standard Randomised Controlled Trial Number registry (ISRCTN): reg. Number: ISRCTN10694967, https://doi.org/10.1186/ISRCTN10694967.
Correlation between Peptacetobacter hiranonis, the baiCD Gene, and Secondary Bile Acids in Dogs
Authors: Correa Lopes Bruna , Chen Chih-Chun , Sung Chi-Hsuan , Ishii Patricia Eri , Medina Luis Fernando da Costa , Gaschen Frederic P. , Suchodolski Jan S. , Pilla Rachel 
Published: January 2024

Bile acid metabolism is a key pathway modulated by intestinal microbiota. Peptacetobacter (Clostridium) hiranonis has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (bai) operon. We aimed to assess the correlation between P. hiranonis abundance, the abundance of one specific gene of the bai operon (baiCD), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of P. hiranonis and baiCD were determined using qPCR. The concentration of fUBA was measured by gas chromatography–mass spectrometry. The baiCD abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), p < 0.0001). Similarly, there was a strong correlation between P. hiranonis and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), p < 0.0001). Animals displaying conversion of fUBA and lacking P. hiranonis were not observed. These results suggest P. hiranonis is the main converter of primary to secondary bile acids in dogs.