bluuebunny/crossref_metadata_2025_split huggingface dataset:
First identification of Microsporidia MB in Anopheles coluzzii from Zinder City, Niger
Authors: Moustapha Lamine Mahaman , Sadou Illiassou Mamane , Arzika Ibrahima Issa , Maman Laminou Ibrahim , Gomgnimbou Michel K. , Konkobo Maurice , Diabate Abdoulaye , Bilgo Etienne 
Published: January 2024

Abstract
Background
Malaria, a disease transmitted by Anopheles mosquitoes, is a major public health problem causing millions of deaths worldwide, mostly among children under the age of 5 years. Biotechnological interventions targeting parasite-vector interactions have shown that the microsporidian symbiont Microsporidia MB has the potential to disrupt and block Plasmodium transmission.

Methods
A prospective cross-sectional survey was conducted in Zinder City (Zinder), Niger, from August to September 2022, using the CDC light trap technique to collect adult mosquitoes belonging to the Anopheles gambiae complex. The survey focused on collecting mosquitoes from three neighborhoods of Zinder (Birni, Kangna and Garin Malan, located in communes I, II and IV, respectively). Collected mosquitoes were sorted and preserved in 70% ethanol. PCR was used to identify host species and detect the presence of Microsporidia MB and Plasmodium falciparum infection.

Results
Of the 257 Anopheles mosquitoes collected and identified by PCR, Anopheles coluzzii was the most prevalent species, accounting for 97.7% of the total. Microsporidia MB was exclusively detected in A. coluzzii, with a prevalence of 6.8% (17/251) among the samples. No significant difference in prevalence was found among the three neighborhoods. Only one An. coluzzii mosquito tested PCR-positive for P. falciparum.

Conclusions
The results confirm the presence of Microsporidia MB in Anopheles mosquitoes in Zinder, Niger, indicating its potential use as a biotechnological intervention against malaria transmission. However, further studies are needed to determine the efficacy of Microsporidia MB to disrupt Plasmodium transmission as well as its impact on vector fitness.

Graphical Abstract
Distribution of Culex pipiens life stages across urban green and grey spaces in Leiden, The Netherlands
Authors: Krol Louie , Langezaal Melissa , Budidarma Lisa , Wassenaar Daan , Didaskalou Emilie A. , Trimbos Krijn , Dellar Martha , van Bodegom Peter M. , Geerling Gertjan W. , Schrama Maarten 
Published: January 2024

AbstractBackgroundThere is an urgent need for cities to become more climate resilient; one of the key strategies is to include more green spaces in the urban environment. Currently, there is a worry that increasing green spaces might increase mosquito nuisance. As such, this study explores a comprehensive understanding of how mosquitoes utilise contrasting grey and green habitats at different life stages and which environmental factors could drive these distributions.MethodsWe used a setup of six paired locations, park (green) vs. residential (grey) areas in a single model city (Leiden, The Netherlands), where we sampled the abundances of different mosquito life stages (eggs, larvae, adults) and the local microclimatic conditions. In this study, we focused onCulex pipienss.l., which is the most common and abundant mosquito species in The Netherlands.ResultsOur results show that whileCx. pipiensovipositioning rates (number of egg rafts) and larval life stages were far more abundant in residential areas, adults were more abundant in parks. These results coincide with differences in the number of suitable larval habitats (higher in residential areas) and differences in microclimatic conditions (more amenable in parks).ConclusionsThese findings suggest thatCx. pipiensdispersal may be considerably more important than previously thought, where adultCx. pipiensseek out the most suitable habitat for survival and breeding success. Our findings can inform more targeted and efficient strategies to mitigate and reduce mosquito nuisance while urban green spaces are increased, which make cities more climate resilient.Graphical Abstract
Avian Plasmodium in invasive and native mosquitoes from southern Spain
Authors: Garrigós Marta , Veiga Jesús , Garrido Mario , Marín Clotilde , Recuero Jesús , Rosales María José , Morales-Yuste Manuel , Martínez-de la Puente Josué 
Published: January 2024

Abstract
Background
The emergence of diseases of public health concern is enhanced by factors associated with global change, such as the introduction of invasive species. The Asian tiger mosquito (Aedes albopictus), considered a competent vector of different viruses and parasites, has been successfully introduced into Europe in recent decades. Molecular screening of parasites in mosquitoes (i.e. molecular xenomonitoring) is essential to understand the potential role of different native and invasive mosquito species in the local circulation of vector-borne parasites affecting both humans and wildlife.

Methods
The presence of avian Plasmodium parasites was molecularly tested in mosquitoes trapped in five localities with different environmental characteristics in southern Spain from May to November 2022. The species analyzed included the native Culex pipiens and Culiseta longiareolata and the invasive Ae. albopictus.

Results
Avian Plasmodium DNA was only found in Cx. pipiens with 31 positive out of 165 mosquito pools tested. None of the Ae. albopictus or Cs. longiareolata pools were positive for avian malaria parasites. Overall, eight Plasmodium lineages were identified, including a new lineage described here. No significant differences in parasite prevalence were found between localities or sampling sessions.

Conclusions
Unlike the invasive Ae. albopictus, Cx. pipiens plays a key role in the transmission of avian Plasmodium in southern Spain. However, due to the recent establishment of Ae. albopictus in the area, further research on the role of this species in the local transmission of vector-borne pathogens with different reservoirs is required.

Graphical Abstract
Apolipoproteins and the risk of giant cell arteritis—a nested case–control study
Authors: Wadström Karin , Jacobsson Lennart T. H. , Mohammad Aladdin J. , Warrington Kenneth J. , Matteson Eric L. , Turesson Carl 
Published: January 2024

Abstract
Background
The etiology of giant cell arteritis (GCA) and its predictors are incompletely understood. Previous studies have indicated reduced risk of future development of GCA in individuals with obesity and/or diabetes mellitus. There is limited information on blood lipids before the onset of GCA. The objective of the study was to investigate the relation between apolipoprotein levels and future diagnosis of GCA in a nested case–control analysis.

Methods
Individuals who developed GCA after inclusion in a population-based health survey (the Malmö Diet Cancer Study; N = 30,447) were identified by linking the health survey database to the local patient administrative register and the national patient register. A structured review of medical records was performed. Four controls for every validated case, matched for sex, year of birth, and year of screening, were selected from the database. Anthropometric measures, self-reported physical activity, based on a comprehensive, validated questionnaire, and non-fasting blood samples had been obtained at health survey screening. Concentrations of apolipoprotein A-I (ApoA-I) and apolipoprotein B (ApoB) in stored serum were measured using an immunonephelometric assay. Potential predictors of GCA were examined in conditional logistic regression models.

Results
There were 100 cases with a confirmed clinical diagnosis of GCA (81% female; mean age at diagnosis 73.6 years). The median time from screening to diagnosis was 12 years (range 0.3–19.1). The cases had significantly higher ApoA-I at baseline screening compared to controls (mean 168.7 vs 160.9 mg/dL, odds ratio [OR] 1.57 per standard deviation (SD); 95% confidence interval [CI] 1.18–2.10) (SD 25.5 mg/dL). ApoB levels were similar between cases and controls (mean 109.3 vs 110.4 mg/dL, OR 0.99 per SD; 95% CI 0.74–1.32) (SD 27.1 mg/dL). The ApoB/ApoA1 ratio tended to be lower in cases than in controls, but the difference did not reach significance. The association between ApoA-I and GCA development remained significant in analysis adjusted for body mass index and physical activity (OR 1.48 per SD; 95% CI 1.09–1.99).

Conclusion
Subsequent development of GCA was associated with significantly higher levels of ApoA-I. These findings suggest that a metabolic profile associated with lower risk of cardiovascular disease may predispose to GCA.
Cost-effectiveness of edaravone dexborneol versus human urinary kallidinogenase for acute ischemic stroke in China
Authors: Chen Pingyu , Luo Mengjie , Chen Yanqiu , Zhang Yanlei , Wang Chao , Li Hongchao 
Published: January 2024

Abstract
Background
Clinical trials have demonstrated the efficacy of edaravone dexborneol in the treatment of acute ischemic stroke. This study aims to determine the cost-effectiveness of edaravone dexborneol compared with human urinary kallidinogenase from China’s healthcare system perspective.

Methods
A combination of the decision tree and Markov model was constructed to evaluate the cost-effectiveness of edaravone dexborneol versus human urinary kallidinogenase in the treatment of acute ischemic stroke over a lifetime horizon. Efficacy data were derived from pivotal clinical trials of edaravone dexborneol and human urinary kallidinogenase (TASTE trial and RESK trial, respectively) and adjusted using matching-adjusted indirect comparison. Cost and health utility inputs were extracted from published literature and open databases. One-way deterministic sensitivity and probabilistic sensitivity analyses were performed to examine the robustness of the results.

Results
Compared with human urinary kallidinogenase, edaravone dexborneol generated 0.153 incremental quality-adjusted life years (QALYs) with an incremental cost of ¥856, yielding an incremental cost-effectiveness ratio of ¥5,608 per QALY gained under the willingness-to-pay threshold (one-time gross domestic product per capita). Both one-way deterministic sensitivity analysis and probabilistic sensitivity analysis demonstrated the robustness of the base case results.

Conclusions
Edaravone dexborneol is a cost-effective treatment choice for acute ischemic stroke patients compared with human urinary kallidinogenase in China.
Effects on mechanical power of different devices used for inhaled sedation in a bench model of protective ventilation in ICU
Authors: Pellet Pierre-Louis , Stevic Neven , Degivry Florian , Louis Bruno , Argaud Laurent , Guérin Claude , Cour Martin 
Published: January 2024

Abstract
Background
Inhaled sedation during invasive mechanical ventilation in patients with acute respiratory distress syndrome (ARDS) has received increasing attention. However, inhaled sedation devices increase dead-space ventilation and an undesirable effect is the increase in minute ventilation needed to maintain CO2 removal. A consequence of raising minute ventilation is an increase in mechanical power (MP) that can promote lung injury. However, the effect of inhaled sedation devices on MP remains unknown.

Methods
We conducted a bench study to assess and compare the effects of three devices delivering inhaled sevoflurane currently available in ICU (AnaConDa-50 mL (ANA-50), AnaConDa-100 mL (ANA-100), and MIRUS) on MP by using a test lung model set with three compliances (20, 40, and 60 mL/cmH2O). We simulated lung-protective ventilation using a low tidal volume and two levels of positive end-expiratory pressure (5 and 15 cmH2O) under ambient temperature and dry conditions. Following the insertion of the devices, either the respiratory rate or tidal volume was increased in 15%-steps until end-tidal CO2 (EtCO2) returned to the baseline value. MP was calculated at baseline and after EtCO2 correction using a simplified equation.

Results
Following device insertion, the EtCO2 increase was significantly greater with MIRUS (+ 78 ± 13%) and ANA-100 (+ 100 ± 11%) than with ANA-50 (+ 49 ± 7%). After normalizing EtCO2 by adjusting minute ventilation, MP significantly increased by more than 50% with all inhaled sedation devices compared to controls. The lowest increase in MP was observed with ANA-50 (p < 0.05 versus ANA-100 and MIRUS). The Costa index, another parameter assessing the mechanical energy delivered to the lungs, calculated as driving pressure × 4 + respiratory rate, significantly increased by more than 20% in all experimental conditions. Additional experiments performed under body temperature, ambient pressure, and gas saturated with water vapor conditions, confirmed the main results with an increase in MP > 50% with all devices after normalizing EtCO2 by adjusting minute ventilation.

Conclusion
Inhaled sedation devices substantially increased MP in this bench model of protective ventilation, which might limit their benefits in ARDS.
Perinatal colonization with extended-spectrum beta-lactamase-producing and carbapenem-resistant Gram-negative bacteria: a hospital-based cohort study
Authors: Styczynski Ashley , Amin Mohammed Badrul , Hoque Kazi Injamamul , Parveen Shahana , Md Pervez Abu Faisal , Zeba Dilruba , Akhter Akhi , Pitchik Helen , Islam Mohammad Aminul , Hossain Muhammed Iqbal , Saha Sumita Rani , Gurley Emily S. , Luby Stephen 
Published: January 2024

Abstract
Background
Antimicrobial resistance (AMR) is a growing global health threat that contributes to substantial neonatal mortality. Bangladesh has reported some of the highest rates of AMR among bacteria causing neonatal sepsis. As AMR colonization among newborns can predispose to infection with these bacteria, we aimed to characterize the frequency of and risk factors for colonization of mothers and newborns during hospitalization for delivery.

Methods
We enrolled pregnant women presenting for delivery to a tertiary care hospital in Faridpur, Bangladesh. We collected vaginal and rectal swabs from mothers pre- and post-delivery, rectal swabs from newborns, and swabs from the hospital environment. Swabs were plated on agars selective for extended-spectrum-beta-lactamase producing bacteria (ESBL-PB) and carbapenem-resistant bacteria (CRB). We performed logistic regression to determine factors associated with ESBL-PB/CRB colonization.

Results
We enrolled 177 women and their newborns during February-October 2020. Prior to delivery, 77% of mothers were colonized with ESBL-PB and 15% with CRB. 79% of women underwent cesarean deliveries (C-section). 98% of women received antibiotics. Following delivery, 98% of mothers and 89% of newborns were colonized with ESBL-PB and 89% of mothers and 72% of newborns with CRB. Of 290 environmental samples, 77% were positive for ESBL-PB and 69% for CRB. Maternal pre-delivery colonization was associated with hospitalization during pregnancy (RR for ESBL-PB 1.24, 95% CI 1.10–1.40; CRB 2.46, 95% CI 1.39–4.37). Maternal post-delivery and newborn colonization were associated with C-section (RR for maternal CRB 1.31, 95% CI 1.08–1.59; newborn ESBL-PB 1.34, 95% CI 1.09–1.64; newborn CRB 1.73, 95% CI 1.20–2.47).

Conclusions
In this study, we observed high rates of colonization with ESBL-PB/CRB among mothers and newborns, with pre-delivery colonization linked to prior healthcare exposure. Our results demonstrate this trend may be driven by intense use of antibiotics, frequent C-sections, and a contaminated hospital environment. These findings highlight that greater attention should be given to the use of perinatal antibiotics, improved surgical stewardship for C-sections, and infection prevention practices in healthcare settings to reduce the high prevalence of colonization with AMR organisms.
Exploring clinical indicator variations in stroke patients with multiple risk factors: focus on hypertension and inflammatory reactions
Authors: Guo Jiejie , Tian Mei , Li Yongang , Guo Yitong , Zhang Ting , Liu Xuan , Shen Jinze , Zhang Lin , Yu Yueqi , Cao Ling , Gu Haiyan , Li Yanfang , Duan Shiwei , Wang Qinwen 
Published: January 2024

Abstract
Background
Stroke stands as the second leading cause of death worldwide. Currently, extensive research has been conducted on stroke risk factors. However, when stroke patients contend with multiple risk factors, the impact on clinical indicators remains uncertain.

Objectives
This study seeks to investigate potential significant variations among distinct ranges of clinical indicators in instances where stroke patients experience multiple risk factors and various ischemic stroke subtypes.

Material and methods
The research encompassed 440 stroke patients admitted to the First People's Hospital of Wenling City, Zhejiang Province, China. These patients were classified based on the type and quantity of risk factors and subtypes of ischemic stroke they presented. The χ2 test was employed to assess the relationship between the risk of comorbid diseases and clinical indicators in stroke patients.

Results
The results of our study have underscored a significant correlation between various comorbid risk factors in stroke patients and the patients' age (P < 0.010). Furthermore, we observed noteworthy disparities in the plasma levels of IL-2, IL-4, IL-6, IL-10, TNF-α, and INF-γ between patients devoid of risk factors and those presenting with comorbid risk factors associated with stroke. Significant differences in INF-γ were observed between the two subtypes of ischemic stroke, namely lacunar infarction and cardioembolic stroke.

Conclusion
Age is correlated with an elevated risk of stroke. Individuals exhibiting multiple stroke risk factors and diverse ischemic stroke subtypes commonly present with abnormal lipid levels and imbalances in Th1/Th2 cytokines. These factors significantly contribute to the onset and progression of stroke. Furthermore, inflammatory responses, particularly those induced by atherosclerosis, play a pivotal role in the genesis of stroke and exert a substantial influence on its prognosis.
Could the minimum wage policy reduce food insecurity among households of formal workers in Indonesia?
Authors: Hasanah Heni , Nachrowi Nachrowi Djalal , Wisana I. Dewa Gede Karma , Siregar Hermanto 
Published: January 2024

Abstract
Background
Previous studies have concluded that minimum wages increase workers' wages. However, whether this effect will continue to improve households' food insecurity is an interesting question, especially in Indonesia, where food insecurity is still a public policy challenge. This study explores the ongoing impact of minimum wages on household food insecurity in Indonesia, leveraging data from the National Socioeconomic Survey (2017–2019) and provincial-level variations in minimum wages. The study employs unconditional quantile regression to provide nuanced insights by analyzing three food insecurity indicators: per capita calorie intake, per capita consumption of vegetables and fruits, and food diversity. We also investigate potential mechanisms driving the link between the minimum wage and food insecurity.

Results
The study revealed that the real minimum wage reduced food insecurity, especially at specific distribution points. Significantly, the effect on per capita calorie intake was observed in lower deciles. The impact on dietary diversity was observed up to the seventh decile. However, the minimum wage increase did not significantly improve the consumption of nutritious foods like fruits and vegetables, except for the top deciles. The study confirmed that the minimum wage's impact on food insecurity operated through wage increases, particularly in the bottom-to-median wage distribution within the manufacturing sector.

Conclusions
The study concluded that the minimum wage policy ameliorated household food insecurity indicators in specific distribution segments. Our results support the effectiveness of government policies in increasing the minimum wage as a viable approach to mitigating food insecurity among formal worker households, especially within the manufacturing sector. However, additional policies targeting the lower end of the per capita calorie intake distribution are necessary, as the minimum wage was recognized to have no impact on this group.
A study on the structural relationship between emotional labor, job burnout, and turnover intention among office workers in Korea: the moderated mediating effect of leader-member exchange
Authors: Li Yiran , You Hyunok , Oh Seokyoung 
Published: January 2024

Abstract
Background
This research investigated the interplay of emotional labor, job burnout, and leader-member exchange on turnover intentions among office workers in South Korea.

Methods
An online survey was conducted with 333 employees working in Korean small- and medium-sized enterprises. The target sample consisted of in-house employees who do not deal with external customers. All the measurement and structural models of this study were analyzed using SPSS 27.0 and Amos 28.0.

Results
The survey revealed that emotional labor indirectly influenced turnover intentions via job burnout and leader-member exchange. Deep acting intensified job burnout, thereby elevating turnover intentions, while surface acting mitigated job burnout.

Conclusions
The findings underscored the importance of managing emotional labor and job burnout and fostering robust leader-member relationships to reduce staff turnover. Moreover, leader-member exchange was found to mitigate the effects of emotional labor on job burnout and turnover intention, with higher leader-member exchange reducing the negative impact of deep acting on turnover intention through job burnout.
R-GNN: recurrent graph neural networks for font classification of oracle bone inscriptions
Authors: Yuan Jiang , Chen Shanxiong , Mo Bofeng , Ma Yuqi , Zheng Wenjun , Zhang Chongsheng 
Published: January 2024

Abstract
Font classification of oracle bone inscriptions serves as a crucial basis for determining the historical period to which they belong and holds significant importance in reconstructing significant historical events. However, conventional methods for font classification in oracle bone inscriptions heavily rely on expert knowledge, resulting in low efficiency and time-consuming procedures. In this paper, we proposed a novel recurrent graph neural network (R-GNN) for the automatic recognition of oracle bone inscription fonts. The proposed method used convolutional neural networks (CNNs) to perform local feature extraction and downsampling on oracle bone inscriptions. Furthermore, it employed graph neural networks (GNNs) to model the complex topologiure and global contextual information of oracle bone inscriptions. Finally, we used recurrent neural networks (RNNs) to effectively combine the extracted local features and global contextual information, thereby enhancing the discriminative power of the R-GNN. Extensive experiments on our benchmark dataset demonstrate that the proposed method achieves a Top-1 accuracy of 88.2%, significantly outperforming the competing approaches. The method presented in this paper further advances the integration of oracle bone inscriptions research and artificial intelligence. The code is publicly available at: https://github.com/yj3214/oracle-font-classification.