bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer Patients' Attitudes Towards the Anti-Covid-19 Vaccine: A
Collective Case Study
Authors: Mirandola Mara , Andreis Federica , Deori Chiara , Abdel Kader Sonia , Wedenissow Anna Chiara , Malighetti Clelia , Meriggi Fausto , Zaniboni Alberto 
Published: November 2023

Aim::
The purpose of the present study was to determine cancer patients’ attitudes
toward the anti-COVID-19 vaccine.

Background::
Historically, the scientific community’s responsibility was to investigate attitudes
about vaccination. The course of COVID-19 in cancer patients makes them a high priority for
vaccination. Cancer patients are at greater risk of serious complications and death because of
COVID-19 infection.

Objective::
The purpose of the present study was to determine cancer patients’ attitudes toward
the anti-COVID-19 vaccine. We examined several constructs that potentially influenced cancer
patients' perceptions of the vaccine: health status, knowledge of COVID-19 and vaccination,
cancer patients' perceptions of vulnerability, and attitudes toward general vaccines.

Methods::
We conducted a collective case study with 200 cancer patients undergoing treatment,
and divided the sample into two groups: patients who “expected to heal” (Group A) and patients
who “expected to chronicize” (Group B). Data were collected through a purpose-built
questionnaire consisting of 22 questions and a study of medical records.

Results::
Data analysis showed that both groups, Group A (M= 3.89 SD= 0.64) and Group B
(M= 3.98 SD= 0.64), had a favorable attitude toward the anti-COVID-19 vaccine. This favorable
attitude toward the anti-COVID-19 vaccine depended on several factors: perception of
vulnerability to COVID-19, perception of the severity of their oncological situation, and
communication with oncologists.

Conclusion::
Our study highlighted the plurality of factors that influence attitudes toward the
anti-COVID-19 vaccine. It is theref+ore of fundamental importance to increase the use of the
shared decision-making approach (SDM) to guide the patient to an informed choice.
Tumor-associated Macrophages Mediate Gefitinib Resistance in Lung Cancer
through HGF/c-met Signaling Pathway
Authors: Tang Xiali , Chen Yu , Jiao Demin , Liu Xiang , Chen Jun , Liu Yongyang , Jiang Chunyan , Chen Qingyong 
Published: November 2023

Background::
The biological behavior of cells changes after they develop drug resistance, and the degree
of resistance will be affected by the tumor microenvironment. In this study, we aimed to study the effects of
M2 macrophages on gefitinib resistance.

Methods:
We polarized THP-1 cells into M0 and M2 macrophages, and conducted various experiments to investigate
the effects of M2 macrophages on gefitinib resistance in lung cancer.

Results:
We found that M2 macrophages promote gefitinib resistance in HCC827 and PC9 cells. In addition, we
used ELISA to measure the secretion level of HGF. HGF secretion levels were significantly increased in M2 macrophages.
Exogenous HGF remarkably increased the proliferation and invasion in HCC827 and PC9 cells. However,
the addition of anti-HGF antibodies abolished the proliferation and invasion of both HCC827 and PC9 cells
promoted by M2 macrophages. Furthermore, M2 macrophages or exogenous HGF significantly increased the
expression of p-met and p-ERK in HCC827 and PC9 cells, while anti-HGF antibodies diminished the expression
of p-met and p-ERK by neutralizing HGF in M2 macrophages.

Conclusion::
Our results revealed that M2 macrophages promote gefitinib resistance by activating ERK and
HGF/c-met signaling pathways in HCC827 and PC9 cells. Our findings provide a new therapeutic strategy for
gefitinib resistance in lung cancer.
Antitumoral Potential of Artepillin C, a Compound Derived from Brazilian Propolis,
against Breast Cancer Cell Lines
Authors: de Freitas Meirelles Lyvia Eloiza , de Assis Carvalho Analine Rosa Barquez , Ferreira Damke Gabrielle Marconi Zago , Souza Raquel Pantarotto , Damke Edilson , de Souza Bonfim-Mendonça Patrícia , de Oliveira Dembogurski Djaceli Sampaio , da Silva Denise Brentan , Consolaro Marcia Edilaine Lopes , da Silva Vania Ramos Sela 
Published: November 2023

Background::
Breast cancer is the most commonly diagnosed cancer among women worldwide with
limited treatment options. Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is one of the main constituents of
Brazilian propolis presenting different activities, including antitumoral effects against various types of cancer.

Objective::
We evaluated the antitumoral potential and mechanisms of action of artepillin C against two distinct
human breast cancer cell lines, MCF-7 and MDA-MB-231, to explore a new therapeutic candidate.

Methods::
Cell viability was assessed by MTT assay and the long-term cytotoxicity was performed by clonogenic
assay. The morphological changes were observed by light microscopy, analysis of cell death pathway by Annexin
V FITC/propidium iodide (PI), lactate dehydrogenase (LDH) by colorimetry, DNA fragmentation by agarose gel
and senescence by β-galactosidase. Detection of total reactive oxygen species (ROS) by fluorescence microscopy
and determination of mitochondrial transmembrane potential by flow cytometry were also performed.

Results::
Artepillin C presented a strong and dose-time-dependent cytotoxic effect on MCF-7 and MDA-MB-231
cell lines, with cytotoxicity more evident in MCF-7. In both cancer cell lines, the clonogenic potential was significantly
reduced and the morphology of the cells was changed. The treatment also induced death by necrosis and
late apoptosis in MCF-7 and MDA-MB-231 and induced cell senescence in MCF-7. Also, artepillin C increased
total ROS in both cancer cells and decreased mitochondrial membrane potential in MDA-MB-231 cells.

Conclusion::
Artepillin C presented antitumoral potential in two human breast cancer cell lines, MCF-7, and
MDA-MB-231, suggesting a new promising option for the treatment and/or chemopreventive strategy for breast
cancer.
Association of Anxiety and Depressive Symptoms with Thyroid Hormone Concentrations in Patients with Primary Bone Tumors
Authors: Ao Man , Xu Kun , Pan Yujun , Wang Shuping , Fu Guoquan 
Published: November 2023

Background::
Timely identification and intervention of psychological disorders bear
significant import in ameliorating the ensuing therapeutic trajectories in primary bone tumor
patients. Moreover, perturbations in thyroxine and thyroid-stimulating hormone (TSH) levels
have been linked to manifestations of depressive and anxiety-related symptoms. However, the
precise interplay governing the nexus of anxiety, depression, and the levels of thyroxine and
TSH within the context of primary bone tumor patients remains presently unexplored.

Objective::
The objective of this study is to investigate the potential correlation between the hypothalamus-
pituitary-thyroxine (HPT) axis and the depressive as well as anxious states observed
in patients afflicted with bone tumors.

Methods::
Patients with primary bone tumors were required to accept the assessments of anxiety
and depressive symptoms as well as thyroid axis hormone concentrations. The depressive and
anxiety symptoms were assessed using the Hamilton Depression Rating Scale (HAMD) and the
Hamilton Anxiety Scale (HAMA) score. During each follow-up, peripheral venous blood samples
were collected for subsequent analysis using radioimmunoassay methods to measure serum-
free T3, free T4, and TSH levels, with the calculated free T3 to free T4 ratio indicating peripheral
free T4 to free T3 conversion. Tests for trend were conducted to assess thyroid axis
hormone concentrations, HAMA scores, and HAMD scores, while the correlation between
HAMA or HAMD scores and thyroid axis hormone concentrations was examined through univariate
regression analyses.

Results::
The study included 30 primary bone tumor patients. Initial high HAMA and HAMD
scores decreased over a year after surgery (P < 0.05), reflecting diminishing anxiety and depression.
TSH levels reduced postoperatively, contrasting with increased free-T3 and free-T4 levels
(p < 0.01). Multivariate analysis affirmed that positive correlations were noted between TSH
and anxiety/depression scores, while free-T3 correlated negatively, adjusted for demographic
factors (p < 0.05). No significant associations emerged between HAMA/HAMD scores and
free-T4 or free-T3 to free-T4 ratio (p > 0.05).

Conclusion::
The early identification of the low T3 syndrome could prove instrumental in both
intervening and preventing adverse emotional states associated with primary bone tumors.
Effectiveness of Blood Glucose Time in Range to Reduce Risk of Blood
Glucose Extrusion and Improve Blood Glucose Metrics in Type 1 Diabetic
Patients
Authors: Oraibi Omar , Somaili Mohammed , Elmakki Erwa , Alqassimi Sameer , Madkhali Mohammed Ali , Mohrag Mostafa , Abusageah Faisal , Alhazmi Mohammed , Alfaifi Samar , Ageeli Ruba , Sumayli Mohammed , Arishi Fatimah , Alhazmi Abdulaziz H. , Hummadi Abdulrahman 
Published: November 2023

Background:
With evolving diabetes technology, continuous glucose monitoring
(CGM) and time in range have been advanced as critical measurements to assess complications.
They have shown improvement in A1C levels and decreased episodes of blood glucose
extrusion.

Aims:
This study aimed to assess the awareness and utilization of blood glucose time in range
and its effectiveness in reducing the risk of blood glucose extrusion and improving blood
glucose metrics among patients with type 1 diabetes mellitus.

Methods:
A retrospective study included 342 patients who met the inclusion criteria and were
using the CGM, aiming for a TIR of 70% daily. Glycemic control was followed using TIR data,
blood glucose extrusion frequency (including hyperglycemia and hypoglycemia events), active
sensor time, average blood glucose, and glucose management indicator (GMI) levels.

Results:
A total of 342 individuals participated in this study, the majority of whom were below
18 years of age (62.3%). The hypoglycemic frequency was significantly increased compared to
the baseline, and most participants experienced hypoglycemia events (p = 0.0001). The
incidences increased over time, with 90.9% and 93% having hypoglycemia at 60 and 90 days (p
= 0.0001), respectively. The active scan and sensor time were not followed, which led to the
blood glucose target not being achieved, with no improvement throughout the study.
Consequently, no improvement occurred in glycemic control.

Conclusion:
CGM technology has been promising and proven effective in improving glycemic.
However, our study did not show these benefits as expected, which could be explained by the
underutilization and improper use of the CGM.
Imaging and Non-imaging Analytical Techniques Used for Drug
Nanosizing and their Patents: An Overview
Authors: Agarwal Vijay , Bajpai Meenakshi 
Published: November 2023

Background:
Nanosizing is widely recognized as an effective technique for improving the
solubility, dissolution rate, onset of action, and bioavailability of poorly water-soluble drugs. To control
the execution and behavior of the output product, more advanced and valuable analytical techniques
are required.

Objective:
The primary intent of this review manuscript was to furnish the understanding of imaging
and non-imaging techniques related to nanosizing analysis by focusing on related patents. In addition,
the study also aimed to collect and illustrate the information on various classical (laser diffractometry,
photon correlation spectroscopy, zeta potential, laser Doppler electrophoresis, X-ray diffractometry, differential
scanning calorimeter, scanning electron microscopy, transmission electron microscopy), new,
and advanced analytical techniques (improved dynamic light scattering method, Brunauer-Emmett-
Teller method, ultrasonic attenuation, biosensor), as well as commercial techniques, like inductively
coupled plasma mass spectroscopy, aerodynamic particle sizer, scanning mobility particle sizer, and matrix-
assisted laser desorption/ionization mass spectroscopy, which all relate to nano-sized particles.

Methods:
The present manuscript has taken a fresh look at the various aspects of the analytical techniques
utilized in the process of nanosizing, and has achieved this through the analysis of a wide
range of peer-reviewed literature. All summarized literature studies provide the information that can
meet the basic needs of nanotechnology.

Results:
A variety of analytical techniques related to the nanosizing process have already been established
and have great potential to weed out several issues. However, the current scenarios require
more relevant, accurate, and advanced analytical techniques that can minimize the time and deviations
associated with different instrumental and process parameters. To meet this requirement, some
new and more advanced analytical techniques have recently been discovered, like ultrasonic attenuation
technique, BET technique, biosensors, etc.

Conclusion:
The present overview certifies the significance of different analytical techniques utilized
in the nanosizing process. The overview also provides information on various patents related to sophisticated
analytical tools that can meet the needs of such an advanced field. The data show that the
nanotechnology field will flourish in the coming future.
Banaba Restricts Brain Damage: Neuroprotective Role in Cerebral Ischemiareperfusion
Injury
Authors: Soni Maneesh , Mandloi Avinash Singh , Baraskar Kapil , Kawadkar Manisha , Dhote Vipin 
Published: November 2023

Background::
Glucose regulation and energy homeostasis mitigate energy crises milieu in reperfusion injury. We investigated Banaba for its outcomes on
cerebral ischemia reperfusion (IR) injury using artery occlusion in rats. The pleiotropic activity of Banaba on various debilitating mechanisms
inducing reperfusion injury was evaluated.

Aim::
This study aimedto evaluate the pharmacological activity of Banaba (Lagerstroemia speciosa) extract on reperfusion injury and investigate the
effect of Banaba on vascular permeability, oxidative stress and cellular damage in ischemia reperfusion injury in rats.

Methods::
Transient ischemia and reperfusion through occlusion of the middle cerebral artery (MCAO) lead to Cerebral IR injury in Wistar rats; it was treated
with oral administration of Banaba extract (100mg/kg and 200mg/kg). The injury outcomes were evaluated after 22 hours of reperfusion by
determining cellular injury, its impact on musculoskeletal coordination, multiple free radical scavenging measures (SOD, GSH, LPO) and vascular
permeability of the blood-brain barrier.

Result::
Banaba treatment led to a marked improvement in neurological outcomes by enhanced coordination and reduced cerebral infarct in comparison to
vehicle control ischemic group. Free radical scavenging activity (SOD and GSH) was significantly better, and lipid peroxidation was reduced by
Banaba treatment; it also reduced the vascular permeability of the blood-brain barrier. We observed that a lower dose of Banaba (100 mg/kg) was
more effective than the higher (200 mg/kg) in ischemic rats. The anti-inflammatory and anti-oxidant activity could drive the neuroprotective
outcomes of Banaba in cerebral IR injury. The critical factor of the beneficial effect of Banaba in cerebral injury is the optimization of dose in this
experimental setup of reperfusion injury using rats.

Conclusion::
The recovery of injury could be attributed to Banaba’s multi-factorial effect targeting free radicals, inflammation, and necrosis during ischemiareperfusion
injury.
Utilization and Awareness of Unani Medicine among Adults in Delhi, India: A Survey-Based Study
Authors: Khan Asim Ali , Janardhanan Rajiv , Kishore Jugal , Saeed Shazina , Das B.C , Parveen Shagufta , Kesharwani Prashant , Karwasra Ritu , Sahebkar Amirhossein , Selvamurthy W. 
Published: November 2023

Objectives::
The objective of this study is to assess the level of knowledge, practice,
and usage of Unani medicine among 250 participants in different areas of New Delhi, India.

Materials and Methods::
A cross-sectional, observational descriptive survey with a sample size
of 250 people was carried out in New Delhi utilizing a pretested, predesigned, and structured
questionnaire to evaluate the awareness and usage patterns of Unani medicine. The study was
conducted at three different spots in different regions of New Delhi.

Results::
With a mean age of 40.056 ± 10.77 years and a range of 18 to 65 years, the 250 participants
were divided into 66% men and 34% women. 60% of the population who had been adopted
had knowledge of Unani medicine, and 54.6% of them relied on Unani medicine as a treatment
regime. 40% of users reported Unani medicine due to lack of no or minimal side effects.
Contrary to this, among non-user (n = 68), 51.4% populace considers Unani medicine as the
slow onset of action medication. Although a small percentage of users (6.1%) chose to use Unani
completely, most preferred it as an adjunct to allopathic care.

Conclusion::
Findings in the survey-based study depict that the awareness of Unani medicines is
better than the utilization pattern. There is an urge and opportunity to advance health education
and change people's perceptions of the Unani medical system.
Improving Adherence to Physical Therapy in the Development of Serious Games: Conceptual Framework Design Study
Authors: Ambros-Antemate Jorge Fernando , Beristain-Colorado María del Pilar , Vargas-Treviño Marciano , Gutiérrez-Gutiérrez Jaime , Hernández-Cruz Pedro Antonio , Gallegos-Velasco Itandehui Belem , Moreno-Rodríguez Adriana 
Published: November 2023

Background
Insufficient levels of treatment adherence can have adverse effects on the outcomes of physical rehabilitation. To address this issue, alternative approaches to traditional therapies, such as serious games, have been designed to enhance adherence. Nevertheless, there remain gaps in the development of serious games concerning the effective implementation of motivation, engagement, and the enhancement of treatment adherence.

Objective
This study aims to design a conceptual framework for the development of serious games that incorporate essential adherence factors to enhance patient compliance with physical rehabilitation programs.

Methods
We formulated a conceptual framework using iterative techniques inspired by a conceptual framework analysis. Initially, we conducted a comprehensive literature review, concentrating on the critical adherence factors in physical rehabilitation. Subsequently, we identified, categorized, integrated, and synthesized the concepts derived from the literature review to construct the conceptual framework.

Results
The framework resembles a road map, comprising 3 distinct phases. In the initial phase, the patient’s characteristics are identified through an initial exploration. The second phase involves the development of a serious game, with a focus on enhancing treatment adherence by integrating the key adherence factors identified. The third phase revolves around the evaluation of the serious game. These phases are underpinned by 2 overarching themes, namely, a user-centered design and the GameFlow model.

Conclusions
The conceptual framework offers a detailed, step-by-step guide for creating serious games that incorporate essential adherence factors, thereby contributing to improved adherence in the physical rehabilitation process. To establish its validity, further evaluations of this framework across various physical rehabilitation programs and user groups are necessary.
Use of Virtual Reality in Interdisciplinary Multimodal Pain Treatment With Insights From Health Care Professionals and Patients: Action Research Study
Authors: Ummels Darcy , Cnockaert Elise , Timmers Inge , den Hollander Marlies , Smeets Rob 
Published: November 2023

Background
Chronic pain is a widespread global health problem. Interdisciplinary multimodal pain treatment (IMPT) is a treatment option for people with chronic pain. Virtual reality (VR) could be used to broaden IMPT programs. However, despite the advantages of VR, it is rarely used in daily clinical practice.

Objective
This research aimed to explore how, when, and with whom VR can be used meaningfully during IMPT, either as an addition or substitution as a component of IMPT.

Methods
This research used an action research design to help health care professionals and patients learn how, when, and with whom they can use VR. Data were collected through reflection sessions with health care professionals and semistructured interviews with patients in 2 specialized centers that provide IMPT for chronic pain. Two researchers performed direct content analyses.

Results
In total, 4 physiotherapists, 1 occupational therapist, 3 psychologists, and 22 patients participated in this research. Three iteration cycles, including 9 reflection sessions and 8 semistructured interviews, were performed. Both health care professionals and patients considered VR to be useful in therapy as an addition but not a substitution. VR was used as a diagnostic and intervention tool with all patients at the rehabilitation center or home. VR was used to gain insight into patients’ pain beliefs, cognitions, and irrational cognitions about additional damage and physical abilities. Considering VR as an intervention tool, the health care professionals had 3 goals: balancing relaxation and competition, grading activities, and exposure in vivo.

Conclusions
VR could be a valuable addition to IMPT for both patients with chronic pain and health care professionals. More research should be performed to assess the additional effects of VR on patients’ participation in daily life.
Gluu Essentials Digital Skills Training for Middle-Aged and Older Adults That Makes Skills Stick: Results of a Pre-Post Intervention Study
Authors: Seaton Cherisse L , Rush Kathy L , Li Eric Ping Hung , Hasan Mohammad Khalad , Fawcus Linda 
Published: November 2023

Background
A number of real-world digital literacy training programs exist to support engagement with mobile devices, but these have been understudied.

Objective
The purpose of this study was to examine the effectiveness and program acceptability of a digital skills training program among middle-aged and older adults (aged ≥50 years) and to gather participants’ recommendations for lifelong digital skills promotion.

Methods
The Gluu Essentials digital skills training program includes learning resources to support tablet use. Through pre-post surveys, this study assessed mobile device proficiency, confidence in going online and in avoiding frauds and scams, the frequency of engaging in online activities, program engagement, acceptability, and suggestions for continued support.

Results
A total of 270 middle-aged and older adults completed baseline surveys. Of these 270 participants, 145 (53.7%) completed follow-up surveys. Our findings indicate that mobile device proficiency increased (P<.001), whereas confidence was unchanged. Participants also reported going online more frequently to shop (P=.01) and access government services (P=.02) at follow-up. Program engagement varied considerably, but program acceptability was high. Participants’ recommendations included the need for providing ongoing programs for support and training because technology constantly changes, reducing costs for technology and internet access, and keeping learning resources simple and easy to access.

Conclusions
The Gluu Essentials digital skills training program increased mobile device proficiency and frequency of web-based activities (shopping and accessing government services) among middle-aged and older adults.