bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1
Authors: Pan Qiaodong , Li Yanhong , Zhang Jing , Hu Ting , Hou Yu , Tang Shen 
Published: February 2024

AbstractMalathion, an extensively used organophosphorus pesticide, poses a high potential risk of toxicity to humans and the environment. Shewanella (S.) oneidensis MR-1 has been proposed as a strain with excellent bioremediation capabilities, capable of efficiently removing a wide range of hard-to-degrade pollutants. However, the physiological and biochemical response of S. oneidensis MR-1 to malathion is unknown. Therefore, this study aimed to examine how S. oneidensis MR-1 responds physiologically and biochemically to malathion while also investigating the biodegradation properties of the pesticide. The results showed that the 7-day degradation rates of S. oneidensis MR-1 were 84.1, 91.6, and 94.0% at malathion concentrations of 10, 20, and 30 mg/L, respectively. As the concentration of malathion increased, superoxide dismutase and catalase activities were inhibited, leading to a significant rise in malondialdehyde content. This outcome can be attributed to the excessive production of reactive oxygen species (ROS) triggered by malathion stress. In addition, ROS production stimulates the secretion of soluble polysaccharides, which alleviates oxidative stress caused by malathion. Malathion-induced oxidative damage further exacerbated the changes in the cellular properties of S. oneidensis MR-1. During the initial stages of degradation, the cell density and total intracellular protein increased significantly with increasing malathion exposure. This can be attributed to the remarkable resistance of S. oneidensis MR-1 to malathion. Based on scanning electron microscopy observations, continuous exposure to contaminants led to a reduction in biomass and protein content, resulting in reduced cell activity and ultimately leading to cell rupture. In addition, this was accompanied by a decrease in Na+/K+- ATPase and Ca2+/Mg2+-ATPase levels, suggesting that malathion-mediated oxidative stress interfered with energy metabolism in S. oneidensis MR-1. The findings of this study provide new insights into the environmental risks associated with organophosphorus pesticides, specifically malathion, and their potential for bioremediation.
Graphical Abstract
Kulturelle Bildung – hausgemacht? Zum Effekt elterlichen kulturellen Kapitals auf die kulturellen Aktivitäten von Jugendlichen
Authors: Burkhard Jannis , Kühne Stefan , Scharf Jan , Maaz Kai 
Published: February 2024

ZusammenfassungIm vorliegenden Beitrag wird aufgezeigt, dass elterliches kulturelles Kapital für den Zugang zu kultureller Bildung von Jugendlichen eine wesentliche Rolle spielt. Es wird argumentiert, dass die intergenerationale Weitergabe von kulturellem Kapital einen Mechanismus darstellt, der ungleiche Chancen auf Teilhabe an kultureller Bildung von Generation zu Generation perpetuieren kann. Der Beitrag stützt sich auf die Kapitaltheorie Pierre Bourdieus und leitet zum einen eine eng daran angelehnte, elaborierte Operationalisierung für kulturelles Kapital her. Zum anderen wird auch kulturelle Bildung differenzierter als üblich in hoch- und soziokulturellen Facetten erfasst, die sowohl formale, non-formale als auch informelle Lernkontexte in allen Sparten einschließen. Die Effekte von elterlichem kulturellem Kapital auf die kulturellen Aktivitäten der Jugendlichen werden auf Basis von NEPS-Daten mit Strukturgleichungsmodellen analysiert. Es zeigt sich, dass hochkulturelle Aktivitäten am stärksten mit der elterlichen Kapitalausstattung zusammenhängen, aber auch außerschulische Kurse, die Teilnahme an Angeboten in Kulturvereinen und der Besuch einer Schule mit musischem Profil, während für kulturelle Aktivitäten in Jugendzentren keine Zusammenhänge mit dem kulturellen Kapital der Eltern nachgewiesen werden können. Die Ergebnisse unterstreichen die Notwendigkeit weiterführender Forschung, um Möglichkeiten aufzuzeigen, wie mehr Chancengleichheit in der kulturellen Bildung herbeigeführt werden kann.
Exosomal circZNF800 Derived from Glioma Stem-like Cells Regulates Glioblastoma Tumorigenicity via the PIEZO1/Akt Axis
Authors: Zhang Ning , Wu Pengfei , Mu Maolin , Niu Chaoshi , Hu Shanshan 
Published: February 2024

AbstractExosomes play a crucial role in regulating crosstalk between tumor and tumor stem-like cells through their cargo molecules. Circular RNAs (circRNAs) have recently been demonstrated to be critical factors in tumorigenesis. This study focuses on the molecular mechanism by which circRNAs from glioma stem-like cell (GSLC) exosomes regulate glioblastoma (GBM) tumorigenicity. In this study, we validated that GSLC exosomes accelerated the malignant phenotype of GBM. Subsequently, we found that circZNF800 was highly expressed in GSLC exosomes and was negatively associated with GBM patients. CircZNF800 promoted GBM cell proliferation and migration and inhibited GBM cell apoptosis in vitro. Silencing circZNF800 could improve the GBM xenograft model survival rate. Mechanistic studies revealed that circZNF800 activated the PIEZO1/Akt signaling pathway by sponging miR-139-5p. CircZNF800 derived from GSLC exosomes promoted GBM cell tumorigenicity and predicted poor prognosis in GBM patients. CircZNF800 has the potential to serve as a promising target for further therapeutic exploration.
Meta-emotion philosophy in teachers from kindergarten to middle school
Authors: Ciucci Enrica , Baroncelli Andrea 
Published: February 2024

AbstractThe present study further investigated the role of teachers as emotional socializers by exploring both the theoretical and the empirical applicability of the construct of meta-emotion philosophy in teachers coming from kindergarten to middle-school. Specifically, we first aimed to test the psychometric properties of a specific tool to assess meta-emotion philosophy (i.e., the Crèche Educator Emotional Style Questionnaire - CEESQ, originally developed for early childhood educators). The second aim was to explore whether the associations between the dimensions provided by the CEESQ were in line with the meta-emotion philosophy framework. 815 female teachers (mean teaching experience = 20.08 years, SD = 10.84 years) from kindergarten to middle school took part to the study. The original 5-factor structure of the CEESQ was confirmed (i.e., three factors related to how teachers approach students’ emotions and perceive their self-efficacy as emotional socializers, and two factors related to how they recognize and approach their own emotions in daily life). Moreover, the CEESQ factors showed several significant interrelations, especially considering the correlations between teachers’ self-efficacy as socializers toward students’ emotions and teachers’ perceptions of their own personal emotional competence. Emerged results indicated the usefulness to expand the construct of meta-emotion philosophy from parenting to student-teacher relationship in order to reach a more comprehensive understanding of the role of teachers as emotional socializers; moreover, they suggest the need for including in teachers’ pre-service and ongoing education a specific attention to promote awareness about the strict connection between personal emotional world and emotion-related educational practices adopted toward students.
Short and decadal impacts of seafloor physical perturbation on the abundances of Lebensspuren ‘traces of life’ in the Peru Basin manganese nodule province
Authors: Vornsand Ina , Boehringer Lilian , Thomsen Laurenz , Purser Autun 
Published: February 2024

AbstractInterest in deep-sea mining for polymetallic nodules as an alternative source to onshore mines for various high-technology metals has risen in recent years, as demands and costs have increased. The need for studies to assess its short- and long-term consequences on polymetallic nodule ecosystems is therefore also increasingly prescient. Recent image-based expedition studies have described the temporal impacts on epi-/megafauna seafloor communities across these ecosystems at particular points in time. However, these studies have failed to capture information on large infauna within the sediments or give information on potential transient and temporally limited users of these areas, such as mobile surface deposit feeders or fauna responding to bloom events or food fall depositions. This study uses data from the Peru Basin polymetallic nodule province, where the seafloor was previously disturbed with a plough harrow in 1989 and with an epibenthic sled (EBS) in 2015, to simulate two contrasting possible impact forms of mining disturbance. To try and address the shortfall on information on transient epifauna and infauna use of these various disturbed and undisturbed areas of nodule-rich seafloor, images collected 6 months after the 2015 disturbance event were inspected and all Lebensspuren, ‘traces of life’, were characterized by type (epi- or infauna tracemakers, as well as forming fauna species where possible), along with whether they occurred on undisturbed seafloor or regions disturbed in 1989 or 2015. The results show that epi- and endobenthic Lebensspuren were at least 50% less abundant across both the ploughed and EBS disturbed seafloors. This indicates that even 26 years after disturbance, sediment use by fauna may remain depressed across these areas.
Single-cell lineage tracing with endogenous markers
Authors: Xue Yan , Su Zezhuo , Lin Xinyi , Ho Mun Kay , Yu Ken H. O. 
Published: February 2024

AbstractResolving lineage relationships between cells in an organism provides key insights into the fate of individual cells and drives a fundamental understanding of the process of development and disease. A recent rapid increase in experimental and computational advances for detecting naturally occurring somatic nuclear and mitochondrial mutation at single-cell resolution has expanded lineage tracing from model organisms to humans. This review discusses the advantages and challenges of experimental and computational techniques for cell lineage tracing using somatic mutation as endogenous DNA barcodes to decipher the relationships between cells during development and tumour evolution. We outlook the advantages of spatial clonal evolution analysis and single-cell lineage tracing using endogenous genetic markers.
Analysis of clinical characteristics, treatment patterns, and outcome of patients with bilateral testicular germ cell tumors
Authors: Azik Hoffman , Omri Nativ , Kamil Malshy , Miki Haifler , Shay Golan , Roy Mano , Yuval Freifeld , Barak Rosenzweig , Ben Shalom , Yariv Stabholz , Reuven Ben-David , Gilad Amiel E. 
Published: February 2024

Abstract
Introduction
Bilateral testicular germ cell tumor (BGCT) is a rare disease, occasionally considered to be more aggressive than unilateral germ cell tumors (GCT) in some reports. Among BGCT, a synchronous disease might be diagnosed at a higher stage than a metachronous disease, resulting in lower cancer-specific survival. Hence, our study aimed to perform a comparative analysis between unilateral testicular GCT, bilateral synchronous GCT, and bilateral metachronous GCT, aiming to verify the possibility that BGCT is diagnosed with a higher stage and may require more aggressive management.

Material and methods
In our multicenter retrospective study we reviewed medical records of 40 patients with BGCT (24 metachronous and 16 synchronous). Clinical characteristics, pathological features of the primary and secondary tumors, adjuvant treatments (chemotherapy and radiotherapy)and sperm quality were evaluated as well as cancer-specific survival and overall survival. A cohort of one-to-one matched patients with unilateral GCT were used to determine risk factors for developing BGCT.

Results
Patients with BGCT were slightly younger compared to those with unilateral GCT and had more advanced disease. Despite similar T-stage distribution between the two groups, nodal involvement was nearly twofold more frequent in patients with BGCT disease (42% vs 22%, p = 0.056). Additionally, although similar histological subtypes distribution at presentation among the two groups, the synchronous disease was diagnosed with a higher local T-stage (OR = 3.4), higher proportions of patients with elevated serum BHCG levels, and more frequent nodal involvement (OR = 2.2). This was later translated into an 18% higher disease-specific mortality rate. The median time to develop a contralateral tumor was 92 months. Pathological local T-stage (T2–T3) of the primary tumor predicted a shorter time interval to a diagnosis of a second contralateral tumor (HR 0.92, P < 0.05).

Conclusion
BGCT presents at a younger age and potentially with more advanced disease. Synchronous BGCT is diagnosed at a later stage compared to metachronous BGCT and has higher disease-specific mortality. Metachronous tumors might have a long time interval for the development of a contralateral neoplasm. The main predictor of developing an early metachronous disease is a high primary T stage.
In-label, off-label prescription, efficacy and tolerability of dalbavancin: report from a National Registry
Authors: Esposito Silvano , Pagliano Pasquale , De Simone Giuseppe , Guarino Amedeo , Pan Angelo , Brambilla Paola , Mastroianni Claudio , Lichtner Miriam , Brugnaro Pierluigi , Carretta Anna , Santantonio Teresa , Brindicci Gaetano , Carrega Giuliana , Montagnani Francesca , Lapadula Giuseppe , Spolti Anna , Luzzati Roberto , Schiaroli Elisabetta , Scaglione Vittoria , Pallotto Carlo , Tacconi Danilo , Quintieri Francesco , Trecarichi Enrico ,   the Italian Society of Infectious, Tropical Diseases
Published: February 2024

Abstract
Purpose
Although dalbavancin is currently approved for the treatment of ABSSIs, several studies suggest its efficacy and tolerance as long-term therapy for other off-label indications requiring prolonged intravenous antibiotic administration.

Methods
We conducted a prospective nationwide study of dalbavancin use in real-life settings for both approved and off-label indications analysing for each case the clinical and microbiological characteristics of infection the efficacy and safety of treatments.

Results
During the study period (from December 2018 to July 2021), the ID specialists from 14 different centres enrolled 223 patients treated with dalbavancin [141 males (63%) and 82 females (37%); male/female ratio 1.72; mean age 59 (SD 17.2) years, (range 15–96). Most patients in the study population (136/223; 61.0%) came from community rather than health care facilities and most of them were visited in Infectious Diseases wards (93/223; 41.7%) and clinics (55/223; 24.7%) even though some patients were cured in other settings, such as surgery wards (18/223; 8.1%), orthopaedic wards (11/223; 4.9%), Emergency Rooms (7/223; 3.1%) and non-surgical other than ID wards (6/223; 2.7%). The most common ID diagnoses were osteomyelitis (44 cases/223; 19.7%; of which 29 acute and 15 chronic osteomyelitis), cellulitis (28/223; 12.5%), cutaneous abscess (23/223; 10.3%), orthopaedic prosthesis-associated infection (22/223; 9.9%), surgical site infection (20/223; 9.0%) and septic arthritis (15/223; 6.7%).

Conclusion
In conclusion, by virtue of its PK/PD properties, dalbavancin represents a valuable option to daily in-hospital intravenous or outpatient antimicrobial regimens also for off-label indications requiring a long-term treatment of Gram-positive infections.
Composition design for modified nickel nanopastes containing a low-melting element as additive for pressureless nanojoining
Authors: Sattler Benjamin , Hausner Susann , Rudolph Nick , Wagner Guntram 
Published: February 2024

AbstractThe use of Ni nanopastes for joining applications offers advantages over other methods. However, since it is based on solid-state processes, a pressure must be applied during the joining process, which represents a challenge. To overcome this, a novel concept is presented in this work, which introduces a low-melting additive to the Ni nanopaste. A liquid phase provides the potential to achieve a joint seam formation with both a reduced porosity and better adhesion to the base material without technical pressure during the process. In a comprehensive selection, elements such as Mg, Al, Ge, In, Sn, and Pr are identified as suitable additives. Their properties in binary phase systems with nickel are discussed. A modified Ni nanopaste with Ge as additive was used in promising initial experiments, which is a good starting point for further investigations.
Age-adjusted visceral adiposity index (VAI) is superior to VAI for predicting mortality among US adults: an analysis of the NHANES 2011–2014
Authors: Liu Wenwu , Weng Shuwei , Chen Yang , Cao Chenghui , Peng Daoquan 
Published: February 2024

Abstract
Background
The association of visceral adiposity with mortality in older adults is conflicting. Whether age influences the predicting ability of visceral adiposity (VAI) for mortality remains unknown. This study uncovered the relationship between age-adjusted visceral adiposity index and mortality through the data of NHANES 2011–2014.

Methods
This study obtained data from the National Health and Nutrition Examination Survey (NHANES) 2011–2014. The age-adjusted visceral adiposity index (AVAI) scores were expressed as quartiles. Receiver operating characteristics (ROC) curve analysis was also applied to compare the predictive ability for mortality. Multivariate weighted Cox regression models were constructed to explore the association between AVAI and mortality. Kaplan–Meier survival curves were conducted for survival analyses. Smooth curve fittings and two-piecewise linear models were applied to explore the relationships between AVAI and mortality.

Results
This study recruited 4281 subjects aged ≥ 18 years from the NHANES 2011–2014. The AUCs of AVAI were 0.82 (0.79, 0.86) and 0.89 (0.85, 0.92) for predicting all-cause mortality and cardiovascular mortality, which were superior to BMI, WC and VAI (all p < 0.05). AVAI is still an independent predictor for mortality adjusted for confounders. The associations of AVAI with all-cause and cardiovascular mortalities were dose-responsive, with higher AVAI scores indicating higher mortality risks.

Conclusion
Age significantly improves the ability of VAI for predicting all-cause and cardiovascular mortality. Age-adjusted VAI is independently associated with mortality risk, and thus could be considered a reliable parameter for assessing mortality risk.
Predictors of hospital readmission rate in geriatric patients
Authors: Bortolani Arianna , Fantin Francesco , Giani Anna , Zivelonghi Alessandra , Pernice Bruno , Bortolazzi Elena , Urbani Silvia , Zoico Elena , Micciolo Rocco , Zamboni Mauro 
Published: February 2024

Abstract
Background
Hospital readmissions among older adults are associated with progressive functional worsening, increased institutionalization and mortality.

Aim
Identify the main predictors of readmission in older adults.

Methods
We examined readmission predictors in 777 hospitalized subjects (mean age 84.40 ± 6.77 years) assessed with Comprehensive Geriatric Assessment (CGA), clinical, anthropometric and biochemical evaluations. Comorbidity burden was estimated by Charlson Comorbidity Index (CCI). Median follow-up was 365 days.

Results
358 patients (46.1%) had a second admission within 365 days of discharge. Estimated probability of having a second admission was 0.119 (95%C.I. 0.095–0.141), 0.158 (95%C.I. 0.131–0.183), and 0.496 (95%C.I. 0.458–0.532) at 21, 30 and 356 days, respectively. Main predictors of readmission at 1 year were length of stay (LOS) > 14 days (p < 0.001), albumin level < 30 g/l (p 0.018), values of glomerular filtration rate (eGFR) < 40 ml/min (p < 0.001), systolic blood pressure < 115 mmHg (p < 0.001), CCI ≥ 6 (p < 0.001), and cardiovascular diagnoses. When the joint effects of selected prognostic variables were accounted for, LOS > 14 days, worse renal function, systolic blood pressure < 115 mmHg, higher comorbidity burden remained independently associated with higher readmission risk.

Discussion
Selected predictors are associated with higher readmission risk, and the relationship evolves with time.

Conclusions
This study highlights the importance of performing an accurate CGA, since defined domains and variables contained in the CGA (i.e., LOS, lower albumin and systolic blood pressure, poor renal function, and greater comorbidity burden), when combined altogether, may offer a valid tool to identify the most fragile patients with clinical and functional impairment enhancing their risk of unplanned early and late readmission.
Disposition of improving quality of life in older adults: the case of Lithuania
Authors: Iždonaitė-Medžiūnienė Inga , Preikšaitienė Laura 
Published: February 2024

Abstract
Background
Quality of life is a phenomenon that recently required lots of concern, especially for older adults, since healthy aging and longevity have become the focus in life. Most research on the quality of life addresses certain issues of older people having special diseases, health problems, and disorders. Our research is based on holistic quality of life empowering multiple areas of life/domains of older adults without addressing their diseases or health disorders.

Aim
Our research aims at evaluating the quality of life of the research participants (older people), addressing their problematic areas and suggestions for better quality of life.

Methods
The conducted research implied mixed methods as quantitative survey and reflection based on interviews. We chose participants from the III age university and the ongoing project “Healthy Aging Program”.

Results
The research results showed the lowest ratings for emotional and physical health and the highest rating for social health. Also, older adults tend to avoid specifying precise measures to improve their quality of life and demonstrate a more conservative stance regarding the implementation of more radical changes in improving water consumption, exercising, meal planning, and enhancing psychosocial well-being.

Conclusions
The overall quality of life rating was lower than the average. Older adults are not sufficiently prepared or educated to make significant changes to develop healthier habits in their quality-of-life improvement, though, they demonstrate concern about their quality of life.
Trends in disability in activities of daily living and instrumental activities of daily living among Chinese older adults from 2011 to 2018
Authors: Ding Hua , Wang Kun , Li Yanan , Zhao Xinyi 
Published: February 2024

Abstract
Aim
This study aimed to assess the trends in disabilities in activities of daily living (ADL) and instrumental activities of daily living (IADL) among older Chinese adults and explore the influence of multimorbidity and unhealthy behaviors on ADL/IADL disability over time.

Methods
Data were obtained from four waves (2011–2018) of the China Health and Retirement Longitudinal Study. Disability in ADL/IADL was defined as inability to perform any ADL/IADL task. Latent class analysis was used to identify multimorbidity patterns. The generalized estimating equation was used to test disability trends. Logistic regression was used to investigate the factors influencing disability.

Results
The prevalence of IADL and ADL disability showed significant increasing trends among older Chinese adults from 2011 to 2018 (ptrend < 0.001). The negative association between alcohol intake more than once per month and IADL disability strengthened over time (ptrend < 0.05). The influence of the “arthritis/digestive diseases” pattern, “cardiometabolic disease” pattern and “high multimorbidity” pattern on ADL disability weakened over time (ptrend < 0.05).

Conclusions
The prevalence of IADL and ADL disability among Chinese older adults increased over time. The “arthritis/digestive diseases” pattern, “cardiometabolic disease” pattern and “high multimorbidity” pattern appeared to be less disabling in ADL over time. Improving the prevention and treatment of multimorbidity and developing age-friendly living conditions could be helpful to reduce the risks of disability.