bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shaking table test of a full-scale PC exterior wall panel connected to a steel frame with flexible node
Authors: Yang Beibei , Li Zhenbao , Liu Ping , Ma Hua , Yang Jiulong , Kang Zhicheng 
Published: November 2023

Abstract
A cantilever block-wall panel attachment strip (CBW) flexible connection node was designed to connect precast concrete (PC) exterior wall panels to steel frames. To investigate the performance of the CBW flexible connection node and PC exterior wall panels during earthquakes, a partial two-storey steel frame was extracted from an actual engineering structure, and a full-scale steel frame-exterior wall panel shaking table model was designed. Two sets of shaking-table tests were conducted under seismic intensity 7, 8, and 9 (Chinese Seismic Intensity Scale) earthquakes. The acceleration and displacement responses of the composite wall panel, open window panel, and integral wall panel along the in-plane and out-of-plane motions were analysed. The acceleration amplification factors of the PC exterior wall panels ranged from 0.753 to 1.400 (in-plane) and from 0.998 to 2.199 (out-of-plane). The CBW flexible connection node had a deformation capacity that could coordinate the deformation of the exterior wall panel and prevent severe damage. The surfaces of the PC exterior wall panels remained intact during a very strong seismic intensity 9 earthquake.
Multiple myeloma-associated light chain proximal tubulopathy: a case report
Authors: Huang# Si-nian , Zhang# Zhi-rong , Zhang Zhi-gang , Zhao Zhong-hua , Yao Jun-xia , Wang Wei-xia 
Published: November 2023

Abstract
Background: Multiple myeloma (MM)-associated light chain proximal tubulopathy (LCPT) mainly damaged the proximal tubules, characterized by the deposition of cytoplasmic inclusions in the proximal tubules.The main lesions of LCPT were proximal tubular damage. Here, we report two cases of LCPT and analyze their pathologic characteristics to delineate the charateristics of clinical pathology and outcomes. Case presentation: A 55-year-old male patient (Case 1) and a 64-year-old male patient(Case 2)were referred to our hospital and diagnosed with MM-associated LCPT with crystalline(κ type) and with non-crystalline LCPT (λ type), respectively.The prominent morphologic feature of crystalline LCPT was the dark-brown granules,which could be detected by using Jones silver methenamine staining,in the proximal tubules through the electron microscopic examination.The typical morphologic-feature of the non-crystalline of LCPT was that the eosinophilic granules,which could be examined by using masson trichrome staining.Besides,an abundance of lysosome could be found by the electron microscopic examination in the non-crystalline of LCPT. The case 1 was treated with 27 cycles of chemotherapy and autologous stem cell transplantation. The treatment of case 1 was effective, and the renal was functioning stably at the 36 months of the follow-up. The case 2 was treated with 44 cycles of chemotherapy. The treatment of case 2 was ineffective. Given the function of case 2’s renal was worsing,dialysis was conducted at 30 months of follow-up.
Conclusions: The morphologic lesions of proximal tubular damage in LCPT is quite variable.Renal biopsy is the first choice for the accurate diagnosis of LCPT. Besides, the clinicopathological features of LCPT are also crucial for accurate pathologic diagnosis and personalized treatment.
Mechanically Robust, Shape-Memory, and Personalized Silk Fibroin/Magnesium Composite Scaffolds induce in situ Irregular Bone Regeneration
Authors: Zheng Yufeng , Mao Zhinan , Bi Xuewei , Yu Chunhao , Chen Lei , Shen Jie , Huang Yongcan , Wu Zihong , Qi Hui , Guan Juan , Shu Xiong , Yu Binsheng 
Published: November 2023

Abstract

The regeneration of critical-sized bone defects, especially for irregular shapes, remain a clinical challenge. Although various biomaterials were developed to enhance bone regeneration, the limitations on the shape-adaptive capacity, the complexity of clinical operation and the unsatisfied osteogenic bioactivity restricted greatly their clinical application. Herein, we construct a mechanically robust, tailorable and blood-responsive shape memory effect silk fibroin/ Magnesium (SF/MgO) composite scaffold, which can quickly match irregular defects by easily trimming and achieve good interface integration. The SF/MgO composite scaffold exhibits excellent mechanical performance and structure retention during the degradation process, which significantly improved the supporting ability in defective site. In addition, the SF/MgO composite scaffolds can achieve shape recovery very quickly either under water or blood conditions. This scaffold promotes cell proliferation, adhesion, migration of osteoblasts, and osteogenic differentiation of BMSCs in vitro. Subcutaneous implantation results confirmed that with suitable content SF/MgO composite scaffold exhibited good histocompatibility, low foreign body reactions, and as well as significant ectopic mineralization and angiogenesis. Meanwhile, the SF/MgO composite scaffold markedly promote in situ robust bone regeneration of cranial defect. This bioactive shape memory porous scaffolds provides a new design idea for minimally invasive clinical-size irregular bone regeneration.
Umbilical cord blood cell characteristics in very preterm neonates for autologous cell therapy of preterm-associated complications
Authors: Zhuxiao Ren , Jiangxue Han , Yongsheng Li , Jingjun Pei , Shuo Yang , Fang Xu , Qi Zhang , Chuan Nie , Jie Yang 
Published: November 2023

Abstract
Background
There are emerging clinical evidence for umbilical cord blood mononuclear cells (UCBMNCs) intervention to improve preterm complications. The first critical step in cell therapy is to obtain high-quality cells. This retrospective study aimed to investigate the quantity and quality of UCBMNCs from very preterm infants (VPIs) for the purpose of autologous cell therapy in prevention and treatment of preterm complications.
Methods
Very preterm infants (VPIs) born in Guangdong Women and Children Hospital from January 1, 2017, to December 8, 2022, from whom cord blood was successfully collected and separated for public or private banking, were enrolled. The UCBMNCs characters from route cord blood tests performed in cord blood bank, impact of perinatal factors on UCBMNCs, the relationship between UCBMNCs characteristics and preterm outcomes, and the correlation of UCBMNCs characteristics and peripheral blood cells in VPIs were analyzed.
Results
Totally, 89 VPIs underwent UCB collection and processing successfully. The median cell number post processing was 2.6×108. To infuse a dose of 5×107 cells/kg, only 3.4% of infants required a volume of more than 20 mL/kg, which exceeded the maximum safe volume limit for VPIs. However, when infusing 10×107 cells/kg, 25.8% of infants required a volume of more than 20 ml/kg volume. Antenatal glucocorticoids use and preeclampsia was associated with lower original UCBMNCs concentration. Both CD34 + hematopoietic stem cells (HSC) frequency and colony forming unit - granulocyte and macrophage (CFU-GM) number correlated negatively with gestational age (GA). UCBMNCs characters had no significant effect on preterm outcomes, whereas a significant positive correlation was observed between UCBMNCs concentration and total white blood cell, neutrophil, lymphocyte and PLT counts in peripheral blood.
Conclusion
UCBMNCs collected from VPIs was feasible for autologous cell therapy in improving preterm complications. Setting the infusion dose of 5×107 cells/kg guaranteed a safe infusion volume in more than 95% of the targeted infants. UCBMNCs characters did not affect preterm complications; however, the effect of UCBMNCs concentration on peripheral blood classification count should be considered when evaluating the immunomodulation of UCBMNCs transfusion.
Keratinocyte-Specific CCN1 Modulation Driven Regulation of Keratinocyte Proliferation and Inflammation in Psoriasis
Authors: Xue Tingting , Zhang Mengjie , Zheng Qi , Luo Ying , Luo Yue , Fei Xiaoya , Song Jiankun , Ding Xiaojie , Kuai Le , Zhang Ying , Li Bin 
Published: November 2023

Abstract
Background: Psoriasis is a chronic autoimmune disease characterized by the hyperproliferation of keratinocytes and inflammatory cell infiltration. CCN1 is a matricellular protein that plays a crucial role in cell proliferation, differentiation, and adhesion, which are accepted as psoriasis-relevant.
Objective: Our study evaluated the function of CCN1 in psoriasis treatment using keratinocyte-specific Ccn1 transgenic mice.
Methods and Results: We constructed keratinocyte tissue-specific Ccn1 transgenic mice and induced a psoriasis model with imiquimod (IMQ). The results exhibited that, compared to the psoriatic model group, the keratinocyte-specific ablation of Ccn1 ameliorated IMQ-induced psoriasis-like skin lesions in mice. Furthermore, the downregulation of PCNA+ cells, NF-kB P50+, F4/80+ macrophage, CD3+ T lymphocytes, and pSTAT3 was confirmed by immunohistochemical staining in the epidermis of psoriatic lesions. Keratinocyte-specific Ccn1 overexpression also increased inflammation in mice after exposure to imiquimod, exhibiting more pronounced red and scaly plaques, epidermal hyperproliferation, and abnormal differentiation of keratinocytes. Thus, targeting CCN1 could broadly improve psoriasis treatment outcomes.
Conclusion: Overall, epidermal-specific CCN1 exerts a key player in keratinocyte proliferation and immunoinflammation in the psoriasis setting. Our study yields valuable insights into the pathogenesis and potential treatment of the disease.
Association between the Demographic Characteristics, Severity, Clinical Symptoms, Vital Signs, Lab Findings with COVID-19 Outcomes in Older Adults
Authors: Ashjazadeh Hamidreza , Zandieh Zhale , Niksolat Maryam , Nabi Somaye , Zanjari Nasibeh , Yarandi Razieh Bidhendi , Azadbakht Mojtaba 
Published: November 2023

Abstract
Introduction: The novel coronavirus 2019 (COVID-19) is an evil infectious disease seriously threatening people's life and health for its high infectivity and incidence. A practical solution to curb the pandemic is to facilitate the recognition of poor prognostic factors at earlier stages, especially in vulnerable groups such as the elderly, to help clinicians make timely decision on treatment initiation.
Methods: This is a retrospective cross-sectional study on information extracted from the medical files of 534 COVID-19 cases aged 60 years and above. Then, the frequencies of these variables by the outcome (recovery or death) were analyzed, and the association between the demographic characteristics, severity, clinical symptoms, vital signs, and underlying health with the mortality rate was reported.
Results: The average age of the population was 72.5±8.77 years. The overall mortality rate was 23.2%. The in-ICU mortality rate of 87%. Mortality increased considerably with a rise in Troponin level, 53% with Troponin≥100. RDW>16, and MCV>96.1 significantly increased mortality. 86% of the cases suffered from chronic diseases. The mortality rate in cases with autoimmune diseases was 64%, and with CKD was 51%.
Conclusions: ICU admission was the strongest predictive indicator for poor outcomes. Mortality increased significantly in the elderly COVID-19 cases suffered from autoimmune diseases, CKD, underlying pulmonary and heart diseases, and diabetes, and those experiencing diastolic and systolic hypotension.
MeSH terms: COVID-19, SARS-CoV-2, Aged, Mortality, Prognosis
Assessing stiffness distribution of gap-graded soils with particle property disparity
Authors: Liu De-Yun , Wang Meng-Ting , Wang Ci 
Published: November 2023

Abstract
This study applies the Discrete Element Method (DEM) to analyze small strain characteristics of gap-graded soils, focusing on particle property disparities between coarse and finer particles. It extends the conventional small strain probe method to assess stiffness distribution in gap-graded specimens, distinguishing between different contact and particle types. Key findings reveal that the disparity in particle properties minimally affects void ratio and coordination number across various densities and stress levels. This disparity also shows limited impact on particle-scale stress transmission, supporting the validity of models with equivalent coarse and finer particle properties. A significant advancement is the adapted small strain probe method, which effectively measures stiffness distribution and demonstrates the role of finer particles, showing that the contribution of finer particles to the small strain stiffness is consistently lower than their volume fraction. This method also captures the effects of particle property disparity on small strain stiffness, demonstrating that higher particle Young’s modulus values in finer particles correlate with increased small strain stiffness contribution. While their overall impact on stiffness is less significant compared to that of stress transmission. This research introduces a simplified approach for analyzing stiffness distribution in gap-graded soils, enhancing understanding of gap-graded soils under diverse conditions.
Clinical Features and Outcomes of Posterior Spinal Artery Infarction: A Systematic Review
Authors: Tan You-Jiang , Teo Tong Lin , Yeo Charlene , Tan Sarah , Tan Youhong , Shah Jinesh 
Published: November 2023

Abstract
Objective
We aim to describe the clinical features and outcomes of patients with posterior spinal artery infarctions, and to identify factors associated with poor ambulatory outcomes.
Methods
We performed a systematic review and retrospective analysis on the characteristics and outcomes of patients with posterior spinal artery infarctions reported over the past 30 years.
Results
We analyzed 40 cases from 33 studies between January 1993 till June 2023. Their median age was 55 years (range 19–84), with 23 males and 17 females. Cardiovascular risk factors were present in half (21/40, 53%). Pain (10/40, 48%) and motor deficits (27/39, 69%) were common Infarcts preferentially affected the cervical cord (29/40, 73%), and were frequently attributed to vertebral artery dissections (8/40, 20%) endovascular treatment complications (7/40, 18%)., However, the underlying cause remained unknown in a quarter (10/40, 25%). Treatment involved isolated or concomitant use of anticoagulants (8/28, 29%), antiplatelet (18/28, 64%), and steroids (5/28, 18%). Prognosis appeared fair as most could ambulate (28/34, 82%), but over a quarter could only do so with assistance (8/28, 29%). Patients who were older, (median age [range]: 67 [52–84] vs 56 [28–79], p = 0.055), with preceding trauma (50% vs 11%, p = 0.053), or with infarcts involving three or more cord segments (83 vs 33%, p = 0.062) may be likelier to experience non-ambulatory outcomes.
Conclusion
Though rare, posterior spinal artery infarctions’ propensity for disability renders their early recognition especially vital. Pain and preceding trauma or endovascular treatment are diagnostic clues. Old age, preceding trauma, and longer infarcts may be associated with non-ambulatory outcomes.
Management of corneal melting after collagen cross-linking for keratoconus: a case report and a review of the literature
Authors: Moramarco Antonio , Geronimo Natalie di , Gardini Lorenzo , Grendele Arianna , Fontana Luigi 
Published: November 2023

Abstract
Purpose:
We describe the management of a case of severe corneal melting after corneal cross-linking (CXL) treated with a staged approach using a conjunctival flap followed by deep anterior lamellar keratoplasty (DALK).
Methods:
A 12-year-old male developed severe corneal melting with pending perforation after an accelerated epithelium-off CXL protocol. We initially treated the patient with a conjunctival flap to prevent perforation. Three months later, we performed DALK to restore vision.
Results:
Conjunctival flap surgery allowed us to avoid corneal perforation and penetrating keratoplasty (PK) à chaud. Once the inflammation had resolved, we recessed the conjunctiva and performed DALK for optical purposes. Twelve months later, the graft was clear and the corrected visual acuity was 20/25 (Snellen). No complications occurred after surgery.
Conclusions:
Although CXL is considered a safe procedure, in rare cases it can lead to serious complications, such as corneal haze, infectious and non-infectious keratitis, stromal melting and perforation. Corneal melting and perforation are usually managed by emergency PK. Herein we suggest a staged approach involving an emergency conjunctival flap followed by DALK at a later time that allowed us to avoid PK à chaud.
Chromosome variation and karyomorphology of Artemisia cina O.Berg: Comparison on Colchicine and 2,4-D + BA Treatment
Authors: Anggraeni Listy , Sulistyaningsih Endang , Purwantoro Aziz 
Published: November 2023

Abstract
In order to produce chromosome doubling in Artemisia cina, we undertook polyploidization. To generate polyploid plants, growth regulators and colchicines were employed. Colchicine treatment (0, 100, 150, 200 ppm) at immersion time of 24 hours, 48 hours, 72 hours, and 96 hours, and treatment 2,4-D and BA (combination of 2,4-D and BA at a concentration of 1–3 ppm) at 21 and 28 days of immersion are used as treatment. Ploidy number, chromosome size, and chromosome karyotype layout were all evaluated.The result of this study shows that a. cina treated with colchicine produced 37 polyploid plants with a distribution of 10.2% triploid, 15.2% tetraploid, 1.7% pentaploid, 1.7% hexaploid, and 0.8% tridecaploid plants. While the plants treated with growth regulators 2,4-D and BA produced 47 polyploid plants with a distribution of 2.5% triploid, 2.3% tetraploid, 1.5% pentaploid, 1.9% hexaploid, 0.4% septaploid, 0.4% octaploid. Plants treated using colchicine produced the most diversity in its ploidy while 2,4-D and BA produced more polyploidy plants and produced mixoploid plants with euploid or aneuploid chromosomes. Karyotype variance are more abundant in tetraploid, and the most uniform karyotype found in pentaploid plants. Colchicine is recommended to produce chromosome doubling rather than growth regulators.
Geospatial techniques for deciphering groundwater potential zones of reservoir-induced seismicity region in western India using analytical hierarchy process
Authors: Ajaykumar Venkatarao , Mondal Nepal Chandra 
Published: November 2023

Abstract
This study aims to demarcate groundwater potential zones using an integrated approach of remote sensing, geographical information system, and analytical hierarchy process in a reservoir-induced seismicity (RIS) region of western India. Multiple important factors affecting groundwater such as geology, geomorphology, soils, land use and land cover, slope, lineaments density, drainage density, rainfall, normalized vegetation index, and topography wetness index were considered for deciphering the groundwater potential zones. Spatially distributed thematic layers of all these factors were generated using remotely sensed data and ground-based field data in GIS platform. The assigned weights of all these layers and their attributes were then normalized by using analytical hierarchy process technique. The deciphered groundwater potential zones of this RIS area were categorized as very good (15.68%), good (27.34%), moderate (29.25%), poor (19.54%), and very poor (8.19%). These assessed groundwater potentialities were positively correlated with the specific yields of actual field data with a correlation coefficient of R = 0.90, and was found reasonable. It was also observed that the very good to good potential zones were in the upstreams. Most of the very good groundwater potential zones (~ 16.79%) were found in the northern part, namely Koyna region (which was more the seismically active) than the Warna region (~ 14.57%) located in the southern part. It indirectly indicated that the groundwater potentially also induced the seismicity of earthquakes along with both Koyna and Warna reservoir waters. The deciphered groundwater potential zones in this RIS area will aid in better study of the earthquake seismicity in future.
In-silico characterization and expression analysis of the carotenoid cleavage dioxygenase1 (ccd1) gene in Maize (Zea mays L.)
Authors: Chanda Sagnik , Roy Jeet , Poudel Ankur , Phogat Sachin , Mulani Ekta , Hossain Firoz , Muthusamy Vignesh , Gaikwad Kishor , Mandal Pranab Kumar , Madhavan Jayanthi , Banu Nuzat 
Published: November 2023

Abstract

To supplement the vitamin A deficiency biofortified maize was developed with increased carotenoid content. However it was observed that during storage carotenoids present in maize grain get degraded and it has been reported that carotenoid cleavage dioxygenase1 (ccd1) is responsible for this degradation. In our current study we characterised the ccd1 gene using in-silico analysis. Expression analysis of ccd1 gene was done in two maize inbreds V335PV and HKI161PV which show significant differences in retention capacity of carotenoid in storage conditions. Both inbred lines showed higher expression of ccd1 during reproductive stages compared to vegetative stages. Furthermore, the milking stage grain exhibited the highest level of ccd1 expression in both inbreds. Hence silencing the ccd1 gene using the recent genome editing tools may be a good strategy for higher carotenoid retention.
Investigation of mass mortality caused by multi-drug resistant Edwardsiella tarda in Oscar fish, Astronotus ocellatus from an ornamental fish farm, Kerala
Authors: Vishnupriya Vijayan , Swaminathan T. Raja , Dharmarathnam Arathi , Sharma S. R Krupesha , Preena P. G 
Published: November 2023

Abstract
Oscar fish (Astronotus ocellatus) is one of the most domesticated and exported ornamental fishes from Kerala. The emergence of diseases caused by multi-drug-resistant bacteria is a serious obstacle faced in the ornamental fish industry. In the present study, six isolates were resolved from the diseased Oscar fish showing hemorrhages, necrosis and loss of pigmentation. After phenotypic and genotypic characterization, the bacteria were identified as Edwardsiella tarda, Klebsiella pneumoniae, Enterococcus fecalis, Escherichia coli, Brevibacillus borstelensis, and Staphylococcus hominis. Experimental challenge studies in healthy Oscar fish showed that E. tarda caused 100% mortality within 240 hours with 6.99×106 CFU/fish as LD50 and histopathology revealed the typical signs of infection. The pathogen could be successfully re-recovered from the moribund fish thereby confirmed Koch’s postulates. Edwardsiella species was confirmed as E. tarda through positive amplification of tarda specific gene and virulence genes viz., etfD and escB were also detected in the pathogen. The isolate showed multiple antimicrobial resistance (AMR) against aminoglycosides, tetracyclines, and quinolones with the MAR index of 0.32, which implicated the antibiotic pressure in the farm. Plasmid curing studies showed a paradigm shift in the resistance pattern with MAR index of 0.04, highlighting the resistance genes are of plasmid-borne. Moreover, tetracycline resistance gene (tetA) was the only one found to be chromosome-borne. This study is the first of its kind in detecting mass mortality caused by E. tarda in Oscar fish. Proper surveillance and appropriate action plans are crucial for the precise detection of pathogens responsible for diseases and AMR in aquaculture.