bluuebunny/crossref_metadata_2025_split huggingface dataset:
An evaluation of sodium loss and gill metal binding properties in rainbow trout and yellow perch to explain species differences in copper tolerance
Authors: Taylor Lisa N. , Wood Chris M. , McDonald D. Gordon 
Published: November 2004

Abstract
The main objective of the study was use a species comparison approach in order understand sensitivity and tolerance differences to copper. We hypothesized that species differences in toxicity would be reflected by differences in copper binding to high-affinity sites on the gill. Specifically, the strength of copper binding (affinity, logK) and maximum number of binding sites (saturation, Bmax) for copper at the gill surface would vary among different species of fish. Two species that are different in their copper sensitivity are the rainbow trout (Oncorhynchus mykiss) and yellow perch (Perca flavescens). We explicitly compared acute toxicity (median lethal concentrations via 96-h LC50s) and whole-body Na+ loss in both organisms in two distinct water chemistries (i.e., hard and soft water). For both species, the copper binding sites at the gill surface were characterized for their affinity and saturability. The binding properties of the gill were quite similar between the two species in each water chemistry. Based on estimations of the free cupric ion concentration, the affinity, or logK, was 8.4 for both species in soft water, whereas in hard water, the affinity was higher (˜9.7). The Bmax value in soft water was 1.88 nmol/g for rainbow trout and yellow perch, while in hard water, saturation occurred at 3.63 nmol/g for rainbow trout and 9.01 nmol/g for yellow perch. More importantly, the amount of copper bound to the gills at 50% mortality (i.e., lethal accumulation; the LA50) was different between the two species (yellow perch LA50s were nine times higher than those of rainbow trout in soft water and hard water), indicating that the copper binding to the yellow perch gill must not have been ‘biologically reactive.’ According to 96-h LC50s, yellow perch were less sensitive to copper than were rainbow trout; however, the difference between the two species was similar in hard water (1.05 vs 4.16 μM) and soft water (˜0.10 vs 0.44 μM). Perch were more tolerant because they lost less sodium upon exposure to copper; yet this mechanism of tolerance was not reflected by the amount of copper at the gill surface. The influence of water chemistry on the binding properties of the gill demonstrates the dynamic nature of the gill in maintaining ionoregulatory homeostasis, a key issue in the future development of the chronic biotic ligand model.
Binding of perfluorinated fatty acids to serum proteins
Authors: Jones Paul D. , Hu Wenyue , De Coen Wim , Newsted John L. , Giesy John P. 
Published: November 2004

Abstract
Perfluorooctane sulfonic acid (PFOS) accumulates in the liver and blood of exposed organisms. The potential for these surfactant molecules to interfere with hormone/protein interactions in blood is of concern given the importance of these interactions. The PFOS binding to serum proteins was investigated by assessing its ability to displace a variety of steroid hormones from specific binding proteins in the serum of birds and fishes. Perfluorooctane sulfonic acid had only a weak ability to displace estrogen or testosterone from carp serum steroid binding proteins. Displacement of cortisone in avian sera occurred at relatively low PFOS concentrations. Corticosterone displacement potency increased with chain length, and sulfonic acids were more potent than carboxylic acids. The PFOS concentrations estimated to cause these effects were 320 μM or greater, equivalent to serum concentrations greater than 160 mg/L. Using mass spectrometry and direct in vitro binding assays, PFOS was demonstrated to bind strongly to bovine serum albumin (BSA) in a 1:1 stoichiometric ratio. It appears that PFOS in serum is in general bound to albumins. Concentrations of PFOS required to saturate albumin would be in excess of 50 to 100 mg/L. Based on current environmental concentrations, it is unlikely that PFOS would cause displacement of hormones from serum proteins in wildlife.
Blood exosome connexins and small RNAs related to demyelinating disease activity
Authors: Maimaitijiang Guzailiayi , Kira Jun‐ichi , Nakamura Yuri , Watanabe Mitsuru , Takase Ezgi Ozdemir , Nagata Satoshi , Sakoda Ayako , Zhang Xu , Masaki Katsuhisa , Yamasaki Ryo , Isobe Noriko , Yamaguchi Hiroo , Imamura Tomohiro 
Published: February 2025

AbstractObjectivesTo assess blood exosome (Ex)‐connexin (Cx)43 (encoded by GJA1) and its truncated isoforms in multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD), which show distinct alterations in astroglial Cx43.MethodsSerum Exs from 48 patients with MS (34 relapsing–remitting, 14 secondary‐progressive), 35 with NMOSD, 20 with other inflammatory neurologic diseases (OIND), and 17 healthy controls (HC) were subjected to quantitative Western blotting for Cx43, single‐molecule array for neurofilament‐L, and quantitative polymerase chain reaction for non‐coding RNAs detected by RNA sequencing. Sera from control and astroglia‐specific Cx43 inducible conditional knockout (Cx43‐icKO) mice with experimental autoimmune encephalomyelitis (EAE) were also tested.ResultsEx‐GJA1‐29k was markedly higher in MS than in NMOSD, OIND, and HC; it successively increased at relapse, remission, and secondary progression, and positively correlated with disability scores. Ex‐hsa‐miR‐133b and other hsa‐miRs that bind to full‐length Cx43 were significantly lower in secondary‐progressive MS than in HC, and Ex‐hsa‐miR‐133b was negatively correlated with disability scores. Ex‐GJA1‐11k expression was lower in NMOSD at relapse than in HC and OIND, and was negatively correlated with disability score worsening and Ex‐neurofilament‐L levels. NMOSD at relapse had significantly higher expression of small nucleolar RNA (SNORD37, SNORD95, and SNORD97) than HC, and SNORD37 and SNORD95 showed strong negative correlations with disability scores. Control mice showed increased Ex‐GJA1‐43k and ‐29k during EAE; this effect was markedly reduced in Cx43‐icKO mice with attenuated EAE.InterpretationBlood Ex‐Cx43‐truncated isoforms and small non‐coding RNAs, which partially come from brain astroglia, are distinctly dysregulated in MS and NMSOD.
Top‐Down Assembly of Photosensitizable Flexible Wood Dressing with Synergistic Effect of Anti‐Infection and Moisture Management
Authors: Huang Siqi , Wang Zaiyu , Wu Qian , Wu Bo , Chen Binbin , Li Ying , Qiu Zijie , Zhao Zheng , Tang Ben Zhong 
Published: February 2025

AbstractInfected wound repair stands as a formidable global issue, posing significant threats to public health and medical care. Biopolymer‐based dressings represent a natural and biocompatible candidate greatly conductive to wound healing. However, the low productivity and high price, coupled with the widespread lack of antibacterial activities, vitally restrict their clinical applications. Herein, a simple yet efficient top‐down assembly strategy is reported to fabricate aggregation‐induced emission photosensitizer (AIE‐PS) functionalized flexible wood (AIE/FW) dressing with synergistic photodynamic therapy and moisture management to accelerate the healing of infected wounds. On account of its superior bactericidal effectiveness and appropriate moisture management, along with superb flexibility and excellent biocompatibility, the proposed AIE/FW can effectively prevent wound infection and alleviate inflammatory response, thereby dramatically boosting the infected wound recovery. It is anticipated that the scalable, sustainable, and cost‐effective AIE/FW offers a promising pathway to overcome global wound care challenges.
A Pre‐Coordinated Strategy Precisely Tailors the Coordination Structure of Single‐Atom Sites Toward Efficient Catalysis
Authors: Cao Fengliang , Zhao Qingshan , Tan Xiaojie , Xu Qian , Wang Libo , Zhu Bingxian , Yan Yue , Kong Debin , Zhi Linjie , Wu Mingbo 
Published: February 2025

AbstractCoordination structure engineering represents a promising approach for optimizing the catalytic properties of single‐atom catalysts (SACs). However, the precise tailoring of single‐atom sites remains challenging. Herein, a pre‐coordination strategy is proposed to design SACs with tunable local coordination environments on 2D honeycomb‐like carbon nanofoams. By pre‐coordinating the metal precursor with customized functional groups on a layered Mg(OH)2 template through strong d‐p orbital hybridization, SACs featuring Co─N4 (Co1/NC), Co─C4 (Co1/CC), and Co─C2S2 (Co1/CSC) configurations are fabricated. The lamellar honeycomb‐like architecture facilitates active site exposure, reactant enrichment, and mass transfer during the reaction process. Consequently, the Co1/NC catalyst, despite its extremely low Co loading of 0.12 wt.%, demonstrates exceptional catalytic activity and stability for nitroaromatics reduction, achieving an impressive overall turnover frequency (TOF) of 73668 h−1 for the conversion of 4‐nitrophenol to 4‐nitroaniline, surpassing most reported catalysts. Theoretical calculations indicate the Co─N4 configuration possesses moderate Fermi electronic states compared to Co─C4 and Co─C2S2, significantly promoting the formation and utilization of reactive H* species and accelerating the reaction kinetics for aromatic nitroreduction. This work establishes a novel avenue for the meticulous manipulation of coordination structures in SACs, paving the way for the advancement of sophisticated catalytic materials for chemical transformations.
Recyclable and Healable Electro‐Optical Fiber for Sensing and Information Transmission
Authors: Li Weikang , Lu Zeren , Zhang Yue , Zhu Liming , Zhang Jiwei , Zhang Yufan , Wu Mengjie , Zhou Xinran , Xiong Jiaqing 
Published: February 2025

AbstractElectro‐optical fibers with dual‐mode sensing ability show broad potential in wearable electronics and intelligent human‐machine interaction. However, the complex multi‐step preparation procedures and the limited environmental adaptivity in materials (stretchability, healability, recyclability, etc.) hinder its practical applications. Herein, based on a urea‐oxime polyurethane, a fiber integrating dual‐mode electrical sensing and two‐color light‐emitting functions is developed using a one‐step continuous coaxial wet‐spinning process, with luminescent sulfides‐doped shell layer and an ionogel conductive core layer. The fiber exhibits excellent mechanical, electrical, and optical healing capabilities with efficiencies of 94%, 92%, and 99%, which can be quickly recycled within 30 minutes. Utilizing the electro‐optical bimodular perceptive fiber, multi‐scenario applications including insect phototaxis monitoring, luminous wearables, and smart tripwires are demonstrated, revealing the healing superiority in programming complex fiber architectures to adapt to substrates with shape or size diversity. Moreover, a healing‐programmed fiber with tailored segments is demonstrated for electro‐optical hybrid encrypted information transmission. This work inspires a promising healing‐programming strategy of healable electro‐optical fibers for wide applications in tactile sensing and information communicating.
Electronic Communication Between Single Atomic Nickel and Iron‐Nitrogen Species Promote the Bifunctional Oxygen Evolution and Reduction for Efficient Rechargeable Zinc‐Air Battery
Authors: Liu Qingqing , Chen Junsheng , Cao Liyun , Wang Ying , Qi Yirong , Wei Ying , Ma Qunzhi , Huang Jianfeng , Fan Xing , Feng Yongqiang 
Published: February 2025

AbstractThe reversible oxygen evolution/reduction reaction (OER/ORR) have been recognized as the key electrochemical process for next‐generation energy conversion and storage (ECS) devices, such as fuel cells and metal‐air batteries. However, the intrinsic large overpotential barrier caused by oxygen‐containing intermediates (*OH, *O, and *OOH) greatly hamper the reaction kinetics of OER/ORR. In this work, a dual‐functional OER/ORR electrocatalyst composed of Ni single atomic sites and FeN0.0324 nanoclusters within a unique core–shell structure of FeN0.0324@NiN4/C is constructed. Benefiting from the efficient synergistic electronic effect of single atomic Ni and FeN0.0324, the FeN0.0324@NiN4/C exhibits excellent electrocatalytic activities for OER with an overpotential of 258 mV at 10 mA cm−2 and ORR with a half‐wave potential (E1/2) of 0.89 V. A liquid zinc‐air battery assembled by FeN0.0324@NiN4/C achieves a maximum peak power density of 180.9 mW cm−2 and cycle endurance stability of more than 150 h. Density functional theory (DFT) calculation indicates that the d‐band center near the Fermi level of NiN4‐FeN4 is shifted more upward in comparison with pristine NiN4H, which effectively optimizes the adsorption of *O and alleviates the troublesome OER process. This study provides a new platform for the construction of new OER/ORR electrocatalysts in the field of energy storage devices.
Bioinspired Nanochitin‐Based Porous Constructs for Light‐Driven Whole‐Cell Biotransformations
Authors: Arumughan Vishnu , Medipally Hitesh , Torris Arun , Levä Tuukka , Grimm Hanna C. , Tammelin Tekla , Kourist Robert , Kontturi Eero 
Published: February 2025

AbstractSolid‐state photosynthetic cell factories (SSPCFs) are a new production concept that leverages the innate photosynthetic abilities of microbes to drive the production of valuable chemicals. It addresses practical challenges such as high energy and water demand and improper light distribution associated with suspension‐based culturing; however, these systems often face significant challenges related to mass transfer. The approach focuses on overcoming these limitations by carefully engineering the microstructure of the immobilization matrix through freeze‐induced assembly of nanochitin building blocks. The use of nanochitins with optimized size distribution enabled the formation of macropores with lamellar spatial organization, which significantly improves light transmittance and distribution, crucial for maximizing the efficiency of photosynthetic reactions. The biomimetic crosslinking strategy, leveraging specific interactions between polyphosphate anions and primary amine groups featured on chitin fibers, produced mechanically robust and wet‐resilient cryogels that maintained their functionality under operational conditions. Various model biotransformation reactions leading to value‐added chemicals are performed in chitin‐based matrix. It demonstrates superior or comparable performance to existing state‐of‐the‐art matrices and suspension‐based systems. The findings suggest that chitin‐based cryogel approach holds significant promise for advancing the development of solid‐state photosynthetic cell factories, offering a scalable solution to improve the efficiency and productivity of light‐driven biotransformation.
Atomic‐Level Modulation of Imine and Cobalt Enables a Homogeneous Co5.47N Catalyst for High‐Performance Lithium‐Sulfur Batteries
Authors: Li Mai , Liu Hui , Cheng Zhi , He Jiawei , Li Huifang , Zhang Lisheng , Liu Tao , Wang Xiaojun , Wang Peng , Liu Zhiming , Cui Guanglei 
Published: February 2025

AbstractElectrocatalyst presents a promising strategy for enhancing sulfur utilization and cycling stability in lithium‐sulfur (Li‐S) batteries, especially under demanding operational conditions. However, conventional catalytic strategies demonstrate undesirable effects in high‐loading lithium‐sulfur batteries, primarily attributed to the absence of efficient catalyst dispersion strategy. In this work, it successfully anchored a diverse range of Co‐containing catalysts uniformly onto the COF‐derived carbon through the unique chelation between cobalt atoms and imine groups. This strategy greatly enhances catalytic efficiency and also prevents the formation of “dead” catalysts, maximizing catalyst utilization. Experimental measurements and simulations reveal that the Co5.47N nanocrystal integrated with COF‐derived carbon (Co5.47N@NC) demonstrates remarkable catalytic efficiency in accelerating polysulfide conversion, primarily owing to the significant modulation of the d‐band center of cobalt atoms within the Co5.47N@NC. Remarkably, the Li‐S battery with Co5.47N@NC achieves a specific capacity of 1288 mAh g−1 at 0.3 C and maintains 92% capacity retention over 200 cycles. The Co5.47N@NC system with a sulfur loading of 5.7 mg cm−2 and an E/S ratio of 4.0 µL mg−1, still sustains a specific capacity of 1314 mAh g−1. Consequently, a 1.0 Ah‐level pouch cell delivers an energy density of 411 Wh kg−1 and maintains stable cycling.
Self‐Supported Ni/Ni(OH)2 Electrodes for High‐Performance Alkaline and AEM Water Electrolysis
Authors: Kuang Wen , Cui Zhixiang , Wang Cheng , Chen Tao , Wang Qingjun , Li Senlin , Yang Tianrang , Liu Jianguo 
Published: February 2025

AbstractA novel tape‐cast Ni/Ni(OH)2 electrode is developed for water electrolysis applications. Through precise control of sintering temperature and in situ formation of Ni(OH)2 nanosheets, the electrode achieves an optimized hierarchical porous structure with interconnected channels, facilitating efficient gas bubble transport and release. The electrode exhibits remarkable hydrogen evolution reaction activity with overpotentials of 40 and 124 mV at 10 and 100 mA cm−2, respectively, comparable to noble metal catalysts. The unique microstructure enables rapid bubble detachment and enhanced mass transport, leading to a high current density of 0.91 A cm−2 at 1.8 V in alkaline water electrolysis. The inherent Ni/Ni(OH)2 composition demonstrates excellent stability over 1500 h of continuous operation and maintains consistent performance under intermittent conditions, making it particularly suitable for renewable energy integration. For anion exchange membrane water electrolysis, the electrode's smooth surface morphology prevents membrane damage while maintaining efficient mass transport, delivering 0.4 A cm−2 at 1.8 V. This study demonstrates that rational design of electrode microstructure through tape‐casting offers a promising pathway toward high‐performance, durable, and cost‐effective water electrolyzers.
High‐Density Catalytic Sites Grafting on 2D‐Metal Oxides for Acceptorless Alcohol Dehydrogenation
Authors: Hong Yu‐Rim , Kumari Nitee , Kim Junwon , Maji Ankur , Kim Min Gyu , Jo Kyu‐Min , Choi Si‐Young , Shim Ji Hoon , Lee In Su 
Published: February 2025

AbstractInstalling well‐defined high density single‐atomic catalyst (SAC) sites is highly desired for the synergistic cooperative effect in efficient chemical synthesis but elusive to synthesize due to unavoidable atomic segregation into clusters or particulates. We implemented a 2D‐nanoconfined SAC grafting strategy. Inside a bilayer silica envelope, single metal hydroxide layer, pre‐loaded with the catalytic metal (Pt, Pd, Ir) precursors, underwent controlled thermal conversion to the homogeneously embedded SACs decorated onto the in situ generated 1 nm‐thin transition metal oxide (TMO) nanosheet. Ultranarrow confined slit‐space exclusively availed laterally emerging cation vacancies of TMO host, as atomic scale sockets to capture SACs within 2D‐plane while vertically restricting the atomic aggregation into nanoparticulates. High density SACs stably decorated on isolated TMO nanosheet, could be obtained in a controllable manner. Well‐defined SACs on TMO were conveniently employed for acceptorless dehydrogenation of a variety of alcohols with quite high activities outperforming reported catalysts. In‐depth, mechanistic investigation revealed the dramatic enhancement in acceptorless dehydrogenation reaction rates by following distinct reaction pathways (concerted or stepwise) depending on the density of SACs cooperativity effect. Such advantageous effect is distinct from the reactions on isolated metal sites in low‐density SACs or nanoparticle surface.
Engineering Lattice Strain in Co‐Doped NiMoO4 for boosting Methanol Oxidation Reaction
Authors: Meenu Preetha Chandrasekharan , Kothoori Naga Pranava Sree , Dahiya Preeti , Mandal Tapas Kumar , Roy Sounak 
Published: February 2025

AbstractNickel‐based molybdates have attracted considerable attention owing to their distinctive isomorphous structure. In this study, pristine NiMoO4 and Co‐doped Ni1‐xCoxMoO4 were synthesized and investigated for their electrocatalytic activity in methanol oxidation and methanol‐assisted water splitting reactions. Through a comprehensive exploration of the structure‐property relationship, it was found that the optimal coexistence of α and β molybdate phases, induced by Co doping, led to lattice strain and facilitated the presence of essential catalytic descriptors such as higher oxidation states of Ni and surface oxygen vacancies within the lattice. These factors contributed to the enhanced electrocatalytic activity of Ni0.7Co0.3MoO4 in methanol oxidation and hydrogen evolution reaction. Detailed kinetic studies were conducted to further elucidate the mechanisms involved. Overall, these findings highlight the promising potential of Ni0.7Co0.3MoO4 as an effective catalyst for electrochemical methanol upgrading in conjunction with water splitting, with implications for sustainable energy conversion technologies.
Unlocking Disease‐Modifying Treatments for TDP‐43‐Mediated Neurodegeneration
Authors: San Gil Rebecca , Walker Adam K. 
Published: February 2025

ABSTRACTNeurons degenerate in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), causing progressive and inevitably fatal neurological decline. The best therapeutic strategies target underlying disease mediators, but after decades of intensive research, the causes of these neurodegenerative diseases remain elusive. Recently, coordinated activities of large consortia, increasing open access to large datasets, new methods such as cryo‐transmission electron microscopy, and advancements in high‐resolution omics technologies have offered new insights into the biology of disease that bring us closer to understanding mechanisms of neurodegeneration. In particular, improved understanding of the roles of the key pathological protein TAR DNA binding protein 43 (TDP‐43) in disease has revealed intriguing new opportunities that provide hope for better diagnostic tools and effective treatments for ALS and FTD.