bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОСОБЛИВОСТІ ТА ПРАВОВЕ РЕГУЛЮВАННЯ ПРАКТИЧНОЇ РЕАЛІЗАЦІЇ ПОЛОЖЕНЬ КПК УКРАЇНИ ЩОДО СКАСУВАННЯ ЗАПОБІЖНОГО ЗАХОДУ ДЛЯ ПРОХОДЖЕННЯ ВІЙСЬКОВОЇ СЛУЖБИ ЗА ПРИЗОВОМ ПІД ЧАС МОБІЛІЗАЦІЇ, НА ОСОБЛИВИЙ ПЕРІОД
Authors: Лазебний А. М. , Теслицький А. А. 
Published: November 2023

У цій статті досліджено нагальну проблему реалізації положень КПК України щодо скасування запобіжного заходу для проходження військової служби за призовом під час мобілізації, на особливий період, що є досить важливим сьогодні в умовах військової агресії Російської Федерації та веденні бойових дій на території України російською регулярною армією та іншими незаконними військовими формуваннями-агресорами. Окремо детально проведено аналіз порядку скасування запобіжного заходу у вигляді тримання під вартою для проходження військової служби.
Під час здійснення мобілізаційних заходів та доукомплектування регулярних частин Збройних сил України та інших військових формувань, відповідно до чинного законодавства, питання скасування запобіжних заходів для підозрюваних / обвинувачених стало досить гостре і набуло суспільного резонансу, тому потребує додаткового дослідження та виявлень недоліків, наскільки ця процедура дає можливість покращити мобільність щодо укомплектування армії, коли це є гострою необхідністю.
Окрема увага приділена нововведеному нормативно-правому забезпеченню для реалізації положень щодо скасування запобіжних заходів для проходження військової служби за призовом під час мобілізації.
З моменту введення воєнного стану до КПК було внесено 8 змін з метою удосконалення кримінального процесу в нових умовах. Це свідчить про різке збільшення динаміки змін до кримінально-процесуального законодавства та необхідність його ретельного аналізу, основним з яких є Закон України № 2125-IX, яким розділ IX-1 КПК України доповнено ст. 616 КПК України. Метою цього Закону є дозвіл реалізувати громадянам України, придатним до військової служби за станом і віком, конституційний обов’язок щодо захисту Вітчизни в умовах воєнного стану. Передумовою такого права стало запровадження в Україні або окремих її місцевостях (на адміністративній території) воєнного стану та здійснення заходів із забезпечення національної безпеки й оборони.
Hybrid dizziness counseling with vestibular rehabilitation in participants with chronic dizziness: a comparison of dizziness handicap
Authors: Chua Kenneth Wei De , Deng Jing , Yuen Heng Wai 
Published: November 2023

IntroductionPatients with chronic dizziness often have an unremarkable laboratory vestibular examination and have medical clearance from other specialties. However, functional impairments are still significant and affect patients' quality of life. Recent diagnostic criteria and identification of persistent postural perceptual dizziness (PPPD) have helped us to better understand the psychological-somatic manifestations of organic disorders. As the literature suggests good efficacy using a combination of cognitive behavior therapy (CBT) and vestibular rehabilitation, we compared the efficacy of a hybrid protocol using dizziness handicap inventory (DHI) as an outcome measure amongst the different sub-types of chronic dizziness.MethodsThis was an observational study with 35 participants allocated to three different groups: those who strictly fulfilled the PPPD criteria, those with spontaneous episodic vestibular syndrome, and participants with non-specific dizziness. We compared the DHI total and sub-domain scores at baseline and 6 months post-intervention for differences. All participants undertook three sessions in 6 months.ResultsThe total DHI scores were reduced in all three groups. However, the DHI total on average was 11 points higher in the episodic vestibular syndrome group. The emotional sub-domain scores were also reduced in all three groups, but the functional and physical scores were significantly higher in the episodic vestibular group.ConclusionA hybrid protocol worked best for typical PPPD patients who strictly fulfilled the criteria followed by participants in the non-specific dizziness group. When symptoms were episodic in spontaneous vestibular syndrome, only emotional handicap was reduced at 6 months, but functional and physical handicap scores remained high. Regardless, the DHI total scores in all groups were significantly reduced, possibly due to CBT reducing the anxiety that results from a lack of understanding of the clinical diagnoses.
Thermal insulation material produced from recycled materials for building applications: cellulose and rice husk-based material
Authors: Marín-Calvo Nacarí , González-Serrud Sergio , James-Rivas Arthur 
Published: November 2023

Construction materials derived from agro-industrial waste are increasingly attractive in the building sector, due to their sustainability and lower environmental impact. Hence, in recent years worldwide the amount of research and publications tending to the development of materials that take advantage of residues from agro-industrial activities has increased. The role of thermal insultation materials in the building envelope is significant, especially in hot-humid region. This study presents the manufacturing and evaluation of a cellulose and rice husk-based insulation material, as a proposal for the reuse of materials considered as value-added waste, such as recycled paper and rice husks. Boards and test specimens were elaborated, as well as mechanical and thermal tests. The material was evaluated by means of thermal tests, in accordance with ASTM C177, to measure the thermal conductivity. Tensile and compressive strength tests were performed, based on ASTM C209 and ASTM C39 Standard, respectively. According to the results obtained, the material shows a thermal coefficient of 0.04 W/m∙K which corresponds to a material with the potential to thermally insulate an enclosure. Maximum stresses were obtained for the 3 compositions in average for a range between 1.31 and 1.76 MPa. Ultimate compressive strength obtained was between 20.19 and 21.23 MPa. The proposed material is presented as a sustainable alternative, which can be used in the field of environmentally friendly buildings, which contribute to reducing the carbon footprint, by energy savings.
Computational identification of potential inhibitors targeting cdk1 in colorectal cancer
Authors: Ogbodo Uchechukwu C. , Enejoh Ojochenemi A. , Okonkwo Chinelo H. , Gnanasekar Pranavathiyani , Gachanja Pauline W. , Osata Shamim , Atanda Halimat C. , Iwuchukwu Emmanuel A. , Achilonu Ikechukwu , Awe Olaitan I. 
Published: November 2023

Introduction: Despite improved treatment options, colorectal cancer (CRC) remains a huge public health concern with a significant impact on affected individuals. Cell cycle dysregulation and overexpression of certain regulators and checkpoint activators are important recurring events in the progression of cancer. Cyclin-dependent kinase 1 (CDK1), a key regulator of the cell cycle component central to the uncontrolled proliferation of malignant cells, has been reportedly implicated in CRC. This study aimed to identify CDK1 inhibitors with potential for clinical drug research in CRC.Methods: Ten thousand (10,000) naturally occurring compounds were evaluated for their inhibitory efficacies against CDK1 through molecular docking studies. The stability of the lead compounds in complex with CDK1 was evaluated using molecular dynamics simulation for one thousand (1,000) nanoseconds. The top-scoring candidates’ ADME characteristics and drug-likeness were profiled using SwissADME.Results: Four hit compounds, namely, spiraeoside, robinetin, 6-hydroxyluteolin, and quercetagetin were identified from molecular docking analysis to possess the least binding scores. Molecular dynamics simulation revealed that robinetin and 6-hydroxyluteolin complexes were stable within the binding pocket of the CDK1 protein.Discussion: The findings from this study provide insight into novel candidates with specific inhibitory CDK1 activities that can be further investigated through animal testing, clinical trials, and drug development research for CRC treatment.
Feature-based clustering of the left ventricular strain curve for cardiovascular risk stratification in the general population
Authors: Ntalianis Evangelos , Cauwenberghs Nicholas , Sabovčik František , Santana Everton , Haddad Francois , Claus Piet , Kuznetsova Tatiana 
Published: November 2023

ObjectiveIdentifying individuals with subclinical cardiovascular (CV) disease could improve monitoring and risk stratification. While peak left ventricular (LV) systolic strain has emerged as a strong prognostic factor, few studies have analyzed the whole temporal profiles of the deformation curves during the complete cardiac cycle. Therefore, in this longitudinal study, we applied an unsupervised machine learning approach based on time-series-derived features from the LV strain curve to identify distinct strain phenogroups that might be related to the risk of adverse cardiovascular events in the general population.MethodWe prospectively studied 1,185 community-dwelling individuals (mean age, 53.2 years; 51.3% women), in whom we acquired clinical and echocardiographic data including LV strain traces at baseline and collected adverse events on average 9.1 years later. A Gaussian Mixture Model (GMM) was applied to features derived from LV strain curves, including the slopes during systole, early and late diastole, peak strain, and the duration and height of diastasis. We evaluated the performance of the model using the clinical characteristics of the participants and the incidence of adverse events in the training dataset. To ascertain the validity of the trained model, we used an additional community-based cohort (n = 545) as external validation cohort.ResultsThe most appropriate number of clusters to separate the LV strain curves was four. In clusters 1 and 2, we observed differences in age and heart rate distributions, but they had similarly low prevalence of CV risk factors. Cluster 4 had the worst combination of CV risk factors, and a higher prevalence of LV hypertrophy and diastolic dysfunction than in other clusters. In cluster 3, the reported values were in between those of strain clusters 2 and 4. Adjusting for traditional covariables, we observed that clusters 3 and 4 had a significantly higher risk for CV (28% and 20%, P ≤ 0.038) and cardiac (57% and 43%, P ≤ 0.024) adverse events. Using SHAP values we observed that the features that incorporate temporal information, such as the slope during systole and early diastole, had a higher impact on the model's decision than peak LV systolic strain.ConclusionEmploying a GMM on features derived from the raw LV strain curves, we extracted clinically significant phenogroups which could provide additive prognostic information over the peak LV strain.
Clinical variables associated with late-onset thrombotic and cardiovascular events, after SARS-CoV-2 infection, in a cohort of patients from the first epidemic wave: an 18-month analysis on the “Surviving-COVID” cohort from Bergamo, Italy
Authors: Benatti S. V. , Venturelli S. , Crotti G. , Ghirardi A. , Binda F. , Savardi M. , Previtali G. , Seghezzi M. , Marozzi R. , Corsi A. , Bonaffini P. A. , Gori M. , Falanga A. , Signoroni A. , Alessio M. G. , Zucchi A. , Barbui T. , Rizzi M. 
Published: November 2023

ImportancePopulation studies have recorded an increased, unexplained risk of post-acute cardiovascular and thrombotic events, up to 1 year after acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.ObjectivesTo search for clinical variables and biomarkers associated with late post-acute thrombotic and cardiovascular events after SARS-CoV-2 infection.DesignRetrospective cohort study.SettingThird-level referral hospital in Bergamo (Italy).ParticipantsAnalysis of an existing database of adult patients, who received care for SARS-CoV-2 infection at our institution between 20 February and 30 September 2020, followed up on a single date (“entry date”) at 3–6 months.ExposureInitial infection by SARS-CoV-2.Main outcomes and measuresPrimary outcome: occurrence, in the 18 months after entry date, of a composite endpoint, defined by the International Classification of Diseases—9th edition (ICD-9) codes for at least one of: cerebral/cardiac ischemia, venous/arterial thrombosis (any site), pulmonary embolism, cardiac arrhythmia, heart failure. Measures (as recorded on entry date): history of initial infection, symptoms, current medications, pulmonary function test, blood tests results, and semi-quantitative radiographic lung damage (BRIXIA score). Individual clinical data were matched to hospitalizations, voluntary vaccination against SARS-CoV-2 (according to regulations and product availability), and documented reinfections in the following 18 months, as recorded in the provincial Health Authority database. A multivariable Cox proportional hazard model (including vaccine doses as a time-dependent variable) was fitted, adjusting for potential confounders. We report associations as hazard ratios (HR) and 95% confidence intervals (CI).ResultsAmong 1,515 patients (948 men, 62.6%, median age 59; interquartile range: 50–69), we identified 84 endpoint events, occurring to 75 patients (5%): 30 arterial thromboses, 11 venous thromboses, 28 arrhythmic and 24 heart failure events. From a multivariable Cox model, we found the following significant associations with the outcome: previous occurrence of any outcome event, in the 18 months before infection (HR: 2.38; 95% CI: 1.23–4.62); BRIXIA score ≥ 3 (HR: 2.43; 95% CI: 1.30–4.55); neutrophils-to-lymphocytes ratio ≥ 3.3 (HR: 2.60; 95% CI: 1.43–4.72), and estimated glomerular filtration rate &lt; 45 ml/min/1.73 m2 (HR: 3.84; 95% CI: 1.49–9.91).Conclusions and relevanceWe identified four clinical variables, associated with the occurrence of post-acute thrombotic and cardiovascular events, after SARS-CoV-2 infection. Further research is needed, to confirm these results.
A cross-sectional analysis of syncytiotrophoblast membrane extracellular vesicles–derived transcriptomic biomarkers in early-onset preeclampsia
Authors: Awoyemi Toluwalase , Zhang Wei , Rahbar Maryam , Cribbs Adam , Logenthiran Prasanna , Jiang Shuhan , Collett Gavin , Cerdeira Ana Sofia , Vatish Manu 
Published: November 2023

BackgroundPreeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%–8% of pregnancies worldwide. Biomarker(s) for the disorder exists, but while these have excellent negative predictive value, their positive predictive value is poor. Extracellular vesicles released by the placenta into the maternal circulation, syncytiotrophoblast membrane extracellular vesicles (STB-EVs), have been identified as being involved in PE with the potential to act as liquid biopsies.ObjectiveThe objective of this study was to identify the difference in the transcriptome of placenta and STB-EVs between preeclampsia and normal pregnancy (NP) and mechanistic pathways.Methods/study designWe performed RNA-sequencing on placental tissue, medium/large and small STB-EVs from PE (n = 6) and NP (n = 6), followed by bioinformatic analysis to identify targets that could be used in the future for EV-based diagnostic tests for preeclampsia. Some of the identified biomarkers were validated with real-time polymerase chain reactions.ResultsOur analysis identified a difference in the transcriptomic STB-EV cargo between PE and NP. We then identified and verified the differential expression of FLNB, COL17A1, SLC45A4, LEP, HTRA4, PAPP-A2, EBI3, HSD17B1, FSTL3, INHBA, SIGLEC6, and CGB3. Our analysis also identified interesting mechanistic processes via an in silico prediction of STB-EV-based mechanistic pathways.ConclusionsIn this study, using comprehensive profiling of differentially expressed/carried genes of three linked sample subtypes in PE, we identified potential biomarkers and mechanistic gene pathways that may be important in the pathophysiology of PE and could be further explored in future studies.
Nano-liposome of thyme essential oil promotes growth performance, antioxidant and immune responses to aeromonad septicemia in Nile tilapia, Oreochromis niloticus, fingerlings
Authors: Yousefi Morteza , Hoseini Seyyed Morteza , Ghadamkheir Maryam , Mahboub Heba H. , Vatnikov Yury Anatolyevich , Kulikov Evgeny Vladimirovich , Sotnikova Elena Dmitriyevna 
Published: November 2023

Nano-encapsulation protects essential oils and increases their efficiency, compared to bulk forms. Hence in the present study, four diets (328 g/kg crude protein and 4402 kcal/kg gross energy) containing 0 (CTL), 25 mg/kg (25TV), 50 mg/kg (50TV), and 100 mg/kg (100TV) thyme, Thymus vulgaris, essential oil nano-liposomes (TV-NP) were offered to Nile tilapia fingerlings (initial weight of 4.27 ± 0.05 g) at water temperature of 26.46 ± 0.43°C, followed by intraperitoneal infection by Aeromonas hydrophila. Three hundred and sixty healthy fish were stocked in 12 tanks (60 L), 30 fish per tank, with daily water renewal rate of 40%. Each diet was offered to three tanks for 70 days. The fish were sampled at the end of feeding period and 12 h after the bacterial challenge. Compared to CTL, 50TV and 100TV treatments exhibited significant elevations in growth rate (14-17%; P&lt;0.001), intestinal activities of amylase (9-19%; P=0.004), lipase (13-26%; P&lt;0.001), protease (20-23%; P=0.001), and post-challenge survival (26-27%; P=0.001). Plasma lysozyme (14-15% P&lt;0.001) and complement (5.1-5.4%; P=0.004) activities significantly increased in 25TV and 50TV, but decreased (lysozyme: 19%, complement 5.9%) in 100TV before the challenge; however, all TV-NP treatments showed similar lysozyme and complement activities after the challenge that were higher than CTL. 50TV and 100TV treatments also showed a decrease in lipid peroxidation (23-26%; P&lt;0.001) and highest glutathione peroxidase activity (17-18%; P=0.001) and pre-challenge superoxide dismutase (21%; P=0.046) and catalase (15-17%; P=0.001) activities. Expression of tumor necrosis factor-alpha (11-fold, P&lt;0.001), inerleukin-1 beta (5-fold, P&lt;0.001), and transforming growth factor-beta (31-fold; P=0.001) in head kidney significantly increased in 100TV before the challenge. After the challenge, the transcripts of the cytokines significantly increased in all treatments and the highest expressions were observed in 50TV and 100TV treatments (62-148-fold). In conclusion, dietary 50-100 mg/kg TV-NP can be considered as a new feed additive in tilapia culture, as it improves growth rate, antioxidant capacity, and disease resistance in the fish.
Dual RNA-Seq reveals transcriptionally active microbes (TAMs) dynamics in the serum of dengue patients associated with disease severity
Authors: Yadav Aanchal , Kumari Pallawi , Devi Priti , Adjele Jorelle Jeanne B. , Budhiraja Sandeep , Tarai Bansidhar , Pandey Rajesh 
Published: November 2023

IntroductionDengue virus (DENV) is a flavivirus that has emerged as a global health threat, characterized by either asymptomatic or mild self-limiting febrile illness, but a subset of DENV outbreaks have been associated with severe disease. Studies have looked into the host immune response and dengue viral load during infection. However, it remains unknown how the active microbial isolates modulate the dengue viral infection. In this study, we demonstrate the significance of in-depth analysis of microbiota composition in the serum samples of dengue-infected patients.Materials and methodsRNA was extracted from the serum samples collected from 24 dengue positive patients. The human mapped reads generated through RNA-Sequencing (RNA-Seq) were removed, while the unmapped (non-human) reads were employed for microbial taxonomic classification using Kraken2 and Bracken2. Further, we assessed the initial blood parameters analyzing the complete blood count (CBC) profile of the patients.ResultsFindings revealed differential abundance of commensals and pathogenic microbes in the early febrile period of hospitalized dengue patients, segregated into, High Viral Reads (HVR) and Low Viral Reads (LVR). The Campylobacter genus was abundant in the HVR whereas Lactobacillus dominated the LVR patients. At species level, the microbiota of HVR exhibited higher abundance of unique potential opportunistic microbes, compared to the commensal microbes’ enrichment in the LVR patients’. We hypothesize that the DENV might alter the microbiota composition as observed by the increase in preponderance of opportunistic pathogens and an absence of commensals in the HVR. The presence of commensals in the LVR might explain, i) overall lower dengue viral reads compared to the HVR, and ii) shift in lymphocytes (high) and neutrophils (low) counts; resulting in a comparatively milder clinical manifestation in this group. Our findings may help in understanding the co-infection aspect that will be important to develop dengue therapeutics and vaccines.DiscussionThis study highlights the potential of the unexplored roles of the TAMs in modulating the dengue disease severity using the metatranscriptomic sequencing. This study serves to enhance our understanding of the distinctive microbial and hematologic signatures in the early infection stage that differentiate patients with high viral reads patients from those with low dengue viral reads.
Machine learning classifiers and associations of cognitive performance with hippocampal subfields in amnestic mild cognitive impairment
Authors: Feng Qi , Wang Luoyu , Tang Xue , Ge Xiuhong , Hu Hanjun , Liao Zhengluan , Ding Zhongxiang 
Published: November 2023

BackgroundNeuroimaging studies have demonstrated alterations in hippocampal volume and hippocampal subfields among individuals with amnestic mild cognitive impairment (aMCI). However, research on using hippocampal subfield volume modeling to differentiate aMCI from normal controls (NCs) is limited, and the relationship between hippocampal volume and overall cognitive scores remains unclear.MethodsWe enrolled 50 subjects with aMCI and 44 NCs for this study. Initially, a univariate general linear model was employed to analyze differences in the volumes of hippocampal subfields. Subsequently, two sets of dimensionality reduction methods and four machine learning techniques were applied to distinguish aMCI from NCs based on hippocampal subfield volumes. Finally, we assessed the correlation between the relative volumes of hippocampal subfields and cognitive test variables (Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment Scale (MoCA)).ResultsSignificant volume differences were observed in several hippocampal subfields, notably in the left hippocampus. Specifically, the volumes of the hippocampal tail, subiculum, CA1, presubiculum, molecular layer, GC-ML-DG, CA3, CA4, and fimbria differed significantly between the two groups. The highest area under the curve (AUC) values for left and right hippocampal machine learning classifiers were 0.678 and 0.701, respectively. Moreover, the volumes of the left subiculum, left molecular layer, right subiculum, right CA1, right molecular layer, right GC-ML-DG, and right CA4 exhibited the strongest and most consistent correlations with MoCA scores.ConclusionHippocampal subfield volume may serve as a predictive marker for aMCI. These findings underscore the sensitivity of hippocampal subfield volume to overall cognitive performance.