bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of myopia and refractive parameters among children and adolescents in Hi-tech District of Chengdu City
Authors: Wang Xiaoqin , Zeng Liuzhi , Xian Yiping , Xin Mei , Shan Qingqing , Li Weiye , Lv Lixia , Liu Yifeng , Zhang Rui , Song Kejian , Tian Xixi , Guo Han , Yan Changjiang , Li Chunyan , Luo Xueni , Luo Honglin , Yang Ling , Luo Jun , Chen Zejun 
Published: February 2025

ObjectiveTo analyze the prevalence of myopia among children and adolescents in Chengdu from 2021 to 2023, providing insights for myopia prevention and control.MethodsThis study was a school-based cross-sectional study in children and adolescents aged 3–18 years in Hi-tech District of Chengdu City. All the students underwent comprehensive ocular measurement, including uncorrected binocular visual acuity (VA), spherical equivalent error (SER) with noncycloplegic autorefraction, corneal radius (CR), and axial length (AL).ResultsOver the three-year study period from 2021 to 2023, the overall prevalence of myopia was 38.15%, with annual rates of 38.74% in 2021, 38.67% in 2022, and 37.03% in 2023. The prevalence and severity of myopia increased significantly with age (P &lt; 0.001). The prevalence was consistently higher among girls (P &lt; 0.001) with 40.17% in 2021, 39.43% in 2022, and 38.33% in 2023 compared to 37.03%, 38.05% and 35.85% among boys in the years, respectively. The myopia prevalence increased with school level (P &lt; 0.001). Mild myopia was the most common (24.47%), followed by moderate (10.77%) and severe myopia (2.91%). The mean SER were −1.72 ± 1.57 D in 2021, −1.32 ± 1.51 D in 2022, and −1.42 ± 1.36 D in 2023 (P &lt; 0.001). AL was stable across most age groups, with mean AL across the overall sample of 23.80 ± 1.02 mm in 2021, 23.79 ± 1.04 mm in 2022, and 23.81 ± 1.04 mm in 2023.ConclusionMyopic prevalence among children and adolescents in Hi-tech District of Chengdu City decreased from 2021 to 2023. The prevalence increased with age and school level, indicating a need for targeted interventions. Significant changes in spherical equivalent refraction and AL emphasize the importance of early intervention and regular monitoring of myopia for an effective management.
Leveraging AI to automate detection and quantification of extrachromosomal DNA to decode drug responses
Authors: Goble Kohen , Mehta Aarav , Guilbaud Damien , Fessler Jacob , Chen Jingting , Nenad William , Ford Christina G. , Cope Oliver , Cheng Darby , Dennis William , Gurumurthy Nithya , Wang Yue , Shukla Kriti , Brunk Elizabeth 
Published: February 2025

IntroductionTraditional drug discovery efforts primarily target rapid, reversible protein-mediated adaptations to counteract cancer cell resistance. However, cancer cells also utilize DNA-based strategies, often perceived as slow, irreversible changes like point mutations or drug-resistant clone selection. Extrachromosomal DNA (ecDNA), in contrast, represents a rapid, reversible, and predictable DNA alteration critical for cancer’s adaptive response.MethodsIn this study, we developed a novel post-processing pipeline for automated detection and quantification of ecDNA in metaphase Fluorescence in situ Hybridization (FISH) images, leveraging the Microscopy Image Analyzer (MIA) tool. This pipeline is tailored to monitor ecDNA dynamics during drug treatment.ResultsOur approach effectively quantified ecDNA changes, providing a robust framework for analyzing the adaptive responses of cancer cells under therapeutic pressure.DiscussionThe pipeline not only serves as a valuable resource for automating ecDNA detection in metaphase FISH images but also highlights the role of ecDNA in facilitating swift and reversible adaptation to epigenetic remodeling agents such as JQ1.
Comparative anti-methanogenic ability of green algae (C. reinhardtii) with/without nanoparticles: in vitro gas and methane production
Authors: Palangi Valiollah , Kaya Adem , Macit Muhlis , Nadaroglu Hayrunnisa , Ünlü Hayrullah Bora , Kaya Ali , Fekri Ashkan , Mammadov Ayaz , Lackner Maximilian 
Published: February 2025

IntroductionThe purpose of this study was to investigate how in vitro gas production (GP) and ruminal fermentation characteristics were affected by increasing concentrations of green algae plant (C. reinhardtii) extracts in combination with nanoparticles MgO and MgS.MethodsA solution containing 0.1 M MgCl2 was prepared in 300 mL for the green production of MgCl nanoparticles. The mixture was refluxed for two hours at 85°C using a reflux condenser after 10 mL of pomegranate plant extract was added. The green algal plant (C. reinhardtii), which has many non-toxic antioxidants, was used as a carbon source to produce carbon quantum dots (CQD). Chemical analysis was conducted in accordance with AOAC (2005) recommendations. Rumen fluid from recently slaughtered calves is used to produce in vitro gas immediately following slaughter. Analysis of variance (ANOVA) was performed on the obtained data from the in vitro study in a completely randomized design using the mixed model of SAS (version 9.4; Inc., Cary NC, USA).Results and DiscussionThe variance analysis results and the average values of the chemical compositions were significantly influenced by the extracts (all p &lt; 0.0001). In this line, the values of net gas, pH, OMD, ME, NEl, and ME were found to be the highest for Algae + 50 MgO and the lowest for Algae + 50 MgS, respectively (all p &lt; 0.0001). These promising results imply that extracts from C. Reinhardtii may be able to mitigate the adverse consequences of rumen fermentation. To precisely ascertain the impact particular Rhodophyta on greenhouse gas emissions, additional investigation is needed.
Comparing Open and Robotic Unilateral Transversus Abdominis Release in Incisional Hernias With a Lateral Component: A Single Center Retrospective Study
Authors: Van Hoef Stijn , Eker Hasan H. , Berrevoet Frederik , Allaeys Mathias 
Published: February 2025

IntroductionLateral hernias are often more challenging to correct when compared to midline defects, due to the anatomic boundaries of the bony pelvis, retroperitoneum, and costal margin. With the insurgence of robot assisted abdominal wall surgery, these defects have been found more manageable through a minimal invasive repair. In this study, we aim to present our short-term results of incisional hernia repair with a lateral component requiring a unilateral transversus abdominis release, through open surgery versus robot assisted.MethodsA retrospective analysis was performed of our robotic and open abdominal wall repairs of lateral hernias, where a unilateral transversus abdominis release was performed, between January 2017 and December 2023. Patient, hernia and perioperative details are reported.Results54 patients in the open group versus 10 patients in the robotic group were included. Hernia width and hernia surface area were higher in the open group, but not significant. Operation time was similar between open and robotic procedures. In-hospital complications, surgical site infection and clinical seroma rate during the first 30 postoperative days were similar in both groups. There was a clear difference in length of stay, in favor of the robotic group.DiscussionIn our limited series, a robotic approach seems safe and feasible when faced with large lateral hernias. Short-term results show a shorter length of stay using the robotic approach, with no significant difference in short term complications, specifically SSI-rate. However, conclusions are limited due to the low number of patients and additional studies should be performed to account for long term recurrence and increase included patient number.
Evaluation of occlusal contacts in antagonist teeth using traditional occlusiography with photographic documentation in patients with periodontitis and TMJ dysfunction during clinical dental appointments
Authors: Redinova T. L. , Redinov I. S. , Chikurova N. V. , Ugolnikov M. А. , Isupov M. V. 
Published: February 2025

Relevance. Selective grinding is incorporated into the comprehensive treatment of periodontitis and TMJ (temporomandibular joint) dysfunction due to the presence of occlusal discrepancies, which can exacerbate or initiate these conditions. The evaluation of occlusal contacts, their distribution, and contact areas is crucial for diagnosing occlusal disturbances and selecting treatment approaches. Traditionally, occlusal contacts have been assessed using occlusograms obtained with articulating paper. However, the advent of digital technologies has made the interpretation of these parameters more challenging for practicing clinicians. This study aimed to evaluate occlusal contact parameters in patients with periodontitis and TMJ dysfunction (without pain symptoms) using traditional occlusiography with photographic documentation and articulating paper during clinical dental appointments.Material and methods. The study included 68 patients aged 18 to 44 years, of whom 41 were divided into four groups: 11 patients without signs of periodontitis or TMJ dysfunction, 11 with TMJ dysfunction but no periodontal pathology, 8 with both periodontitis and TMJ dysfunction, and 11 with periodontitis but no TMJ dysfunction. Occlusal contacts and their surface areas were assessed using occlusiography with photographic documentation.Results. The study revealed an increase in the number of occlusal contacts in cases of periodontitis and TMJ dysfunction. In patients with periodontitis and combined pathology (periodontitis and TMJ dysfunction), a significant rise in the frequency of pathological tooth wear was observed. The occlusal contact area varied significantly across all groups.Conclusion. An analysis of the literature and the obtained data identified consistent trends in changes in the number and area of occlusal contacts associated with these clinical conditions. The "occlusal contact area" parameter exhibited considerable variability and warrants further research to establish reference values. No conclusive data supporting selective grinding in patients with periodontitis and TMJ dysfunction were obtained.
Exosomes Derived from Dialysis Patients Serum enhance Endothelial-Mesenchymal Transition and Calcification in Endothelial Cells
Authors: Cohen-Hagai Keren , Kuchuk Eran , Matalon Shelly Tartakover , Benchetrit Sydney , Zitman-Gal Tali 
Published: February 2025

Background:
Vascular calcification (VC) is prevalent among patients with end stage kidney disease (ESKD). Exosomes, small extracellular vesicles actively secreted by cells, contain proteins, nucleic acids, lipids and other bioactive substances and are considered major mediators of cell-cell interactions. Endothelial-Mesenchymal Transition (EndMT) has been observed in a variety of pathological conditions, such as abnormal shear stress, vascular damage, and chronic inflammation. The aim of this research was to assess the effects of serum-derived exosomes from ESKD patients with VC on the induction of EndMT in endothelial cells and their potential role in accelerating VC.

Methods:
Twenty hemodialysis patients with VC and 10 healthy volunteers were recruited. Cardiac and brain VC were assessed among patients with ESKD treated with dialysis. Serum samples were taken at dialysis initiation for exosome isolation. Human umbilical vein endothelial cells (HUVEC) were treated with 100 µg/mL exosomes for 24-96 hours. At the end of incubation, cells were collected for mRNA and protein analysis.

Results:
Exosomes isolated from dialysis patients with VC induced EndMT in HUVECs. After 24h, endothelial markers CD31 and VE-cadherin were decreased (31% and 51%, respectively; P<0.001) and the mesenchymal proteins Vimentin and N-cadherin were increased (283% and 156%, respectively; P<0.001), compared to healthy exosomes. After 96h of incubation, expression of genes essential for osteoblast differentiation, including the bone morphogenetic genes (BMP2, BMPR2, BMP4 and BMP9), and the transcription factor RUNX2 were significantly elevated.

Conclusions:
Exosomes derived from the serum of dialysis patients with VC, induced EndMT and contributed to calcification. The vicious cycle highlighted the intricate interplay between exosomes, EC and VC, emphasizing the critical necessity for therapeutic strategies to disrupt this pathway and mitigate calcification advancement.
Peripheral Transcriptomics in Acute and Long-Term Kidney Dysfunction in SARS-CoV2 Infection
Authors: Jayaraman Pushkala , Rajagopal Madhumitha , Paranjpe Ishan , Suarez-Farinas Mayte , Liharska Lora , Thompson Ryan , Del Valle Diane Marie , Beckmann Noam , Lund Anina N. , Gownivaripally Pooja , Oh Wonsuk , Gulamali Faris F. , Kauffman Justin , Gonzalez-Kozlova Edgar , Dellepiane Sergio , Vasquez-Rios George , Vaid Akhil , Jiang Joy , Fox Ben , Sakhuja Ankit , Chen Steven , Kenigsberg Ephraim , He John Cijiang , Coca Steven G. , Chan Lili , Merad Miram , Kim-Schulze Seunghee , Gnjatic Sacha , Tsalik Ephraim , Langley Raymond , Charney Alexander W. , Nadkarni Girish N. 
Published: February 2025

Background:
Acute kidney injury (AKI) is common in SARS-CoV-2 infection and COVID-19, often leading to long-term kidney dysfunction. However, the transcriptomic features of AKI severity and its long-term effects are underexplored.

Methods:
We performed bulk RNA sequencing on peripheral blood mononuclear cells (PBMCs) from hospitalized SARS-CoV-2 patients and complemented these findings with proteomic data from the same cohort. We compared the functional enrichment findings with historical sepsis-AKI data and subsequently examined the association between molecular signatures and long-term kidney function changes.

Results:
In 283 patients, 57 had mild AKI (stage 1) and 49 had severe AKI (stage 2 or 3). Following adjustments for age, sex, severity of infection, and pre-existing chronic kidney disease (CKD), we identified 6,432 differentially expressed genes (DEGs) in the severe AKI vs. control comparison, 840 in the mild AKI vs. control, and 1,213 in the severe vs. mild AKI comparison (FDR<0.05). Common pathways included unfolded protein response, cellular response to stress via eIF2, and IFN-g-mediated inflammatory response. Severe AKI was linked to pathways involved in mitochondrial dysfunction and endoplasmic reticulum stress. Proteomic analysis confirmed 40 established AKI and inflammation biomarkers, while gene-set enrichment of transcription regulators revealed additional biomarkers for severe AKI. Comparison with PBMC transcriptomics from sepsis-related AKI showed significant functional overlap (30%). Analysis of post-discharge eGFR data in 115 patients identified 177 DEGs for severe vs. control, 106 for mild vs. control, and 46 for severe vs. mild AKI. Key associations included kidney function decline related to carbohydrate and mitochondrial metabolism, inflammatory-response, and cardiovascular regulation.

Conclusions:
We demonstrate that severe AKI in SARS-CoV-2 infection is linked to mitochondrial dysfunction and ER stress. The functional overlap with sepsis-AKI suggests potential broader therapeutic applicability. Long-term kidney dysfunction is influenced by disruptions in cellular energy metabolism and immune response.
Peran Pendidikan Islam dalam Membangun Kepribadian Anak di Lingkungan Keluarga
Authors: Nurdin  , Nur Tajuddin 
Published: February 2025

Tujuan dari penelitian ini untuk mendeskripsikan peran pedidikan Islam di lingkungan keluarga masyarakat Karawang dalam membangun kepribadian anak. Metode penelitian ini merupakan studi lapangan deskriptif. Penelitian ini bersifat kualitatif. Pengumpulala data menggunakan strategi informan dimana pengumpulan data berasal dari wawancara langsung dengan sumber yang menggunakan teknik bola salju, yaitu, dimulai dengan sejumlah informan tertentu dan kemudian berkembang menjadi semakin banyak individu (satu orang di setiap bidang penelitian). Hasil penelitian menunjukkan dalam kehidupan masyarakat Karawang, nilai-nilai Islam tidak dapat dipisahkan, dan dalam hal mendidik anak-anak dalam keluarga, semuanya memiliki nuansa Islam, berdasarkan tuntunan dan ajaran agama Islam untuk membentuk anak menjadi manusia yang beriman dan bertaqwa kepada Allah SWT untuk menghindari larangan-Nya (amar ma’ruf dan nahi mungkar ), sesuai dengan Al-Qur'an. Peran keluarga dalam menghasilkan generasi unggul melalui konsep Islam konsisten dengan prinsip-prinsip keislaman. Kesimpulannya peran pendidikan Islam dalam lingkungan keluarga masyarakat Karawang memberikan dampak positif dan bisa menjadi buruk bila tidak diterapkan terhadap pendidikan anak dalam membangun kepribadian anak sehingga terjadi pergeseran nilai-nilai budaya pada masyarakat Karawang.
Kemampuan Mengolah Bahan Bacaan pada Penyusunan Proposal Skripsi Mahasiswa
Authors: Widowati Tutuk , Putera Zulmy Faqihuddin , Shofiah Nurul , Ramadhani Rizki Putri 
Published: February 2025

Tujuan penelitian ini adalah untuk mendeskripsikan kemampuan mengolah bacaan melalui ketrampilan membaca kritis dan menulis kritis mahasiswa dalam penyusunan proposal skripsi. Metode penelitian ini menggunakan pendekatan deskriptif kualitatif. Data penelitian ini merupakan proposal skripsi yang dihasilkan oleh mahasiswa tingkat akhir D4 yang berjumlah 40 mahasiswa. Sumber data dalam penelitian ini adalah mahasiswa tingkat akhir D4 Program Studi Elektronika Politeknik Negeri Malang. Data penelitian dikumpulkan dengan menggunakan teknik dokumentasi. yaitu mengumpulkan proposal skripsi mahasiswa tingkat akhir D4 untuk mengetahui kemampuan mengolah bahan bacaan bagi mahasiswa. Instrumen penelitian yang digunakan dalam penelitian yaitu pedoman membaca kritis dan keterampilan menulis kritis. Dari hasil penelitian dapat disimpulkan bahwa kemampuan Pertama, kemampuan membaca kritis mahasiswa yang menonjol pada aspek menginterpretasi dengan indikator mengenali sumber bacaan sesuai dengan topik, menemukan informasi faktual, dan menilai kredibilitas informasi atau pendapat. Kemampuan membaca kritis yang perlu diperhatikan lagi yakni ketrampilan menganalisis dengan indikator membandingkan atau mengontraskan ide, konsep atau pernyataan dan mengidentifikasi isu ataumasalah dan menentukan hubung. Kedua, gambaran kemampuan menulis kritis yang menonjol pada keterampilan mensintesis dan yang perlu diperhatikan kemampuan mengevaluasi.
Pengembangan Model Project Based Learning Skema Weekly Project of Problem sebagai Pendukung Merdeka Belajar Peserta Didik Kelas XI SMAN 1 Bululawang
Authors: Mahmudi Prasetiyo M. , Amanda Putri Sagita , Nur Haliza Jubaida , Abdul Halim Fathani  
Published: February 2025

Project Based Learning. merupakan model pembelajaran untuk memecahkan segala permasalahan dengan menyusun suatu proyek yang terstruktur sehingga peserta didik dapat aktif belajar bersama pendidik. Mengembangkan skema terapan melalui Weekly Project of Problem atau bisa disingkat dengan W-PoP agar berkesinambungan dengan model Project Based Learning sehingga membangkitkan interaksi belajar namun tidak terpusat pada pendidik. . Penelitian ini bertujuan untuk menghasilkan sebuah skema Weekly Project of Problem, mendeskripsikan kelayakan Weekly Project of Problem berbasis PjBL dan mengetahui pengaruh skema Weekly Project of Problem untuk mengembangkan kemampuan bermatematika dan berpikir kritis siswa kelas XI SMAN 1 Bululawang sebagai pendukung merdeka belajar. Metode Research & Development (R&D) padapenelitian ini dilaksanakan dengan tahapan sebagai berikut: a) analisis awal, b) perencanaan media pembelajaran, c) pengembangan prototipe, d) implementasi, e) Diseminasi dan sosialisasi produk akhir. Hasil penelitian menunjukkan bahwa (1) Skema Weekly Project of Problem berbasis Project Based Learning telah dikembangkan sesuai dengan konsep Borg & Gall, (2) dinyatakan layak oleh 3 guru penguji penelitian dengan menghasilkan rerata nilai 85, (3) berdasarkan perbandingan pretest, posttest dan paired sample t-test dari penerapan skema Weekly Project of Problem berbasis Project Based Learning telah memberikan pengaruh pada pembelajaran yang lebih baik.
Pengembangan Buku Panduan Permainan Tradisional untuk Karakter Tanggung Jawab Sosial Anak Usia 10-12 Tahun
Authors: Mahendra Andreas Linggar , Nugrahanta Gregorius Ari 
Published: February 2025

Penelitian ini difokuskan pada fenomena perilaku anak yang kurang tanggung jawab dan memiliki sifat sosialisasi rendah, mencerminkan rendahnya pendidikan karakter yang seharusnya diperkenalkan secara lebih mendalam pada anak-anak. Dengan fokus tersebut, penelitian ini bertujuan untuk mengembangkan buku panduan permainan tradisional sebagai sarana menumbuhkan karakter tanggung jawab dan kecakapan sosial tinggi pada anak berusia 10-12 tahun. Metode penelitian yang digunakan adalah Research and Development (R&D) dengan tipe ADDIE. Sepuluh guru sekolah dasar yang sudah bersertifikasi dari berbagai daerah yaituYogyakarta, Sleman, Bantul, Klaten, Batang, dan Bali, terlibat dalam analisis kebutuhan. Penilai ahli adalah sepuluh validator dan delapan anak sebagai peserta dalam uji terbatas. Hasil penelitian menunjukkan bahwa pengembangan buku panduan dilakukan melalui lima tahap ADDIE. Buku panduan pendidikan karakter mencapai kualitas "Sangat Baik" dengan skor 3,82. Selain itu, buku panduan menumbuhkan karakter anak dalam peningkatan tanggung jawab dan kecakapan sosial tinggi dengan nilai sebesar t (7) = 24,949, p= 0,000, p < 0,05. Pengaruh besar dari penerapan permainan tradisional Nusantara tergambar dengan r = 0,994 atau setara dengan 98%, termasuk dalam kategori "Efek Besar." Keefektifan buku panduan juga terkonfirmasi melalui N-Gain Score sebesar 86,47%, menunjukkan tingkat keefektifan yang tinggi.
Pengembangan Media Pembelajaran IPA Berbasis Literasi Sains pada Materi Sistem Tata Surya di SMPS Santo Agustinus
Authors: Anu Melania Krisanta , Kua Maria Yuliana , Ariyani Ni Wayan Prawita , Dinata Ngurah Mahendra 
Published: February 2025

Rendahnya literasi sains menyebabkan siswa menjadi kurang tanggap terhadap perkembangan dan permasalahan yang ada di sekitar lingkungan terutama yang berkaitan dengan fenomena alam yang berada di lingkungan sekitar. Penelitian ini bertujuan menggambarkan tahapan pengembangan media pembelajaran IPA berbasis literasi sains pada materi system tata surya. Jenis penelitian yang digunakan adalah Research and Development model ADDIE yang terdiri dari lima tahap yaitu (Analysis, Design, Development, Implementation, Evaluation). Subjek dari penelitian ini adalah siswa siswi SMP Swasta Santo Agustinus. Hasil dari penelitian yang dilakukan di SMP Swasta Santo Agustinus menunjukan bahwa media pembelajaran IPA berbasis literasi sains pada materi sistem tata surya valid dan sangat layak digunakan pada pembelajaran IPA.
Analisis Kebutuhan Pengembangan Media Ajar Etno-Vlog Batik Gentongan Tanjung Bumi Madura pada Siswa SMP
Authors: Martareza Ajeng Wahyu , Hadi Wiwin Puspita , Fikriyah Aida 
Published: February 2025

Tujuan dari penelitian ini yaitu untuk menganalisis kebutuhan pengembangan media pembelajaran berupa Etno-Vlog batik Gentongan tanjung Bumi Madura pada pembelajaran sains di Sekolah Menengah Pertama (SMP). Metode penelitian yang digunakan yaitu berupa pendekatan deskriptif kuantitatif dengan menggunakan metode pengumpulan data berupa survei yang dilakukan kepada 174 orang siswa di kelas VIII pada SMPN 1 Tanjungbumi. Teknik pengambilan data yang digunakan adalah dokumentasi, wawancara, angket. Hasil penelitian menunjukkan bahwa siswa memberikan respons yang positif terhadap upaya pengembangan media pembelajaran berupa Etno-Vlog untuk diterapkan pada kegiatan pembelajaran khususnya pada materi “Unsur, Senyawa, & Campuran” hasil tersebut menunjukkan bahwa penelitian ini perlu untuk terus dilanjutkan sehingga dapat menghasilkan produk berupa media pembelajaran berupa Etno-Vlog untuk dapat diaplikasikan pada kegiatan pembelajaran.