bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcription factor WOX11 modulates tolerance to cyst nematodes via adventitious lateral root formation
Authors: Willig Jaap-Jan , Guarneri Nina , van Loon Thomas , Wahyuni Sri , Astudillo-Estévez Ivan E , Xu Lin , Willemsen Viola , Goverse Aska , Sterken Mark G , Lozano-Torres José L , Bakker Jaap , Smant Geert 
Published: February 2024

Abstract
The transcription factor WUSCHEL-RELATED HOMEOBOX 11 (WOX11) in Arabidopsis (Arabidopsis thaliana) initiates the formation of adventitious lateral roots upon mechanical injury in primary roots. Root-invading nematodes also induce de novo root organogenesis leading to excessive root branching, but it is not known if this symptom of disease involves mediation by WOX11 and if it benefits the plant. Here, we show with targeted transcriptional repression and reporter gene analyses in Arabidopsis that the beet cyst nematode Heterodera schachtii activates WOX11-mediated adventitious lateral rooting from primary roots close to infection sites. The activation of WOX11 in nematode-infected roots occurs downstream of jasmonic acid-dependent damage signaling via ETHYLENE RESPONSE FACTOR109, linking adventitious lateral root formation to nematode damage to host tissues. By measuring different root system components, we found that WOX11-mediated formation of adventitious lateral roots compensates for nematode-induced inhibition of primary root growth. Our observations further demonstrate that WOX11-mediated rooting reduces the impact of nematode infections on aboveground plant development and growth. Altogether, we conclude that the transcriptional regulation by WOX11 modulates root system plasticity under biotic stress, which is one of the key mechanisms underlying the tolerance of Arabidopsis to cyst nematode infections.
Dual plastid targeting of protoporphyrinogen oxidase 2 in Amaranthaceae promotes herbicide tolerance
Authors: Wittmann Daniel T , Peter Franziska E , Strätker Sarah Melissa , Ortega-Rodés Patricia , Grimm Bernhard , Hedtke Boris 
Published: February 2024

Abstract
Plant tetrapyrrole biosynthesis (TPB) takes place in plastids and provides the chlorophyll and heme required for photosynthesis and many redox processes throughout plant development. TPB is strictly regulated, since accumulation of several intermediates causes photodynamic damage and cell death. Protoporphyrinogen oxidase (PPO) catalyzes the last common step before TPB diverges into chlorophyll and heme branches. Land plants possess two PPO isoforms. PPO1 is encoded as a precursor protein with a transit peptide, but in most dicotyledonous plants PPO2 does not possess a cleavable N-terminal extension. Arabidopsis (Arabidopsis thaliana) PPO1 and PPO2 localize in chloroplast thylakoids and envelope membranes, respectively. Interestingly, PPO2 proteins in Amaranthaceae contain an N-terminal extension that mediates their import into chloroplasts. Here, we present multiple lines of evidence for dual targeting of PPO2 to thylakoid and envelope membranes in this clade and demonstrate that PPO2 is not found in mitochondria. Transcript analyses revealed that dual targeting in chloroplasts involves the use of two transcription start sites and initiation of translation at different AUG codons. Among eudicots, the parallel accumulation of PPO1 and PPO2 in thylakoid membranes is specific for the Amaranthaceae and underlies PPO2-based herbicide resistance in Amaranthus species.
Effect of PCDH19 missense mutations on cell-to-cell proximity and neuronal development under heterotypic conditions
Authors: Motosugi Nami , Sugiyama Akiko , Otomo Asako , Sakata Yuka , Araki Takuma , Hadano Shinji , Kumasaka Natsuhiko , Fukuda Atsushi 
Published: February 2024

Abstract
The mutation of the X-linked protocadherin (PCDH) 19 gene in heterozygous females causes epilepsy. However, because of the erosion of X-chromosome inactivation (XCI) in female human pluripotent stem cells, precise disease modeling often leads to failure. In this study, using a mathematical approach and induced pluripotent stem cells retaining XCI derived from patients with PCDH19 missense mutations, we found that heterotypic conditions, which are composed of wild-type and missense PCDH19, led to significant cell-to-cell proximity and impaired neuronal differentiation, accompanied by the aberrant accumulation of doublecortin, a microtubule-associated protein. Our findings suggest that ease of adhesion between cells expressing either wild-type or missense PCDH19 might lead to aberrant cell aggregation in early embryonic phases, causing poor neuronal development.
Buprenorphine Receipt and Retention for Opioid Use Disorder Following an Initiative to Increase Access in Primary Care
Authors: Hawkins Eric J. , Malte Carol A. , Hagedorn Hildi J. , Gordon Adam J. , Williams Emily C. , Trim Ryan S. , Blanchard Brittany E. , Lott Aline , Danner Anissa N. , Saxon Andrew J. 
Published: February 2024

ObjectivesBuprenorphine, a medication for opioid use disorder (OUD), is underutilized in general medical settings. Further, it is inequitably received by racialized groups and persons with comorbidities. The Veterans Health Administration launched an initiative to increase buprenorphine receipt in primary care. The project's objective was to identify patient-related factors associated with buprenorphine receipt and retention in primary care clinics (n = 18) participating in the initiative.MethodsRetrospective cohort quality improvement evaluation of patients 18 years or older with 2 or more primary care visits in a 1-year period and an OUD diagnosis in the year before the first primary care visit (index date). Buprenorphine receipt was the proportion of patients with OUD who received 1 or more buprenorphine prescriptions from primary care providers during the post–index year and retention the proportion who received buprenorphine for 180 days or longer.ResultsOf 2880 patients with OUD seen in primary care, 11.7% (95% confidence interval [CI], 10.6%–12.9%) received buprenorphine in primary care, 58.2% (95% CI, 52.8%–63.3%) of whom were retained on buprenorphine for 180 days or longer. Patients with alcohol use disorder (adjusted odds ratio [AOR], 0.39; 95% CI, 0.27–0.57), nonopioid drug use disorder (AOR, 0.64; 95% CI, 0.45–0.93), and serious mental illness (AOR, 0.60; 95% CI, 0.37–0.97) had lower buprenorphine receipt. Those with an anxiety disorder had higher buprenorphine receipt (AOR, 1.42; 95% CI, 1.04–1.95). Buprenorphine receipt (AOR, 0.55; 95% CI, 0.35–0.87) and 180-day retention (AOR, 0.40; 95% CI, 0.19–0.84) were less likely among non-Hispanic Black patients.ConclusionsFurther integration of addiction services in primary care may be needed to enhance buprenorphine receipt for patients with comorbid substance use disorders, and interventions are needed to address disparities in receipt and retention among non-Hispanic Black patients.
Endometrial Cancer in a Transgender Man with Prolonged Exogenous Testosterone Use
Authors: Gill Stephanie , Anderson Michelle , Neveu Joannie 
Published: February 2024

BACKGROUND:
Hormone therapy (HT) in transgender males requires monitoring. For amenorrheic transmasculine individuals on HT, episodes of abnormal vaginal bleeding should be assessed promptly.

CASE:
A 33-year-old transgender man on exogenous testosterone therapy for medical gender transition was found to have stage IV endometrioid endometrial adenocarcinoma. Surgical resection was performed for symptom control, and the patient was treated with palliative chemotherapy. The tumor was androgen receptor–negative, and, after a multidisciplinary discussion of the risks and benefits of continuing exogenous testosterone, testosterone therapy was restarted postoperatively.

CONCLUSION:
Long-term androgen use may have unknown implications for the development of malignancy, and treating reproductive organ cancer in transgender males may be complicated by the desire to continue androgen therapy.
Neonatal Birth Weight With Daily Compared With Every-Other-Day Glucose Monitoring in Gestational Diabetes Mellitus
Authors: Feldman Kristina Martimucci , Coughlin Arielle , Feliciano Jasmin , Stoffels Guillaume , Wang Kelly Z. , Strauss Tirtza Spiegel , Grubman Olivia , Al-Ibraheemi Zainab , Cole David , Ashmead Graham , Hussain Farrah , Shchur Sophia Scarpelli , Lee Deborah , Brustman Lois 
Published: February 2024

OBJECTIVE:
To assess whether universal use of every-other-day glucose monitoring in patients with gestational diabetes mellitus (GDM) resulted in similar birth weights and medication use and was preferred by the patient compared with traditional daily glucose monitoring.

METHODS:
This was a noninferiority randomized controlled trial conducted at a single New York City hospital between April 2021 and May 2022. Patients with singleton pregnancies who were diagnosed with GDM after 20 weeks of gestation and had a minimum of 7 days of previous daily blood glucose testing were randomly assigned to test blood glucose values daily or every other day. The primary outcome was neonatal birth weight. We calculated a total sample size of 196 participants needed for noninferiority to be tested, assuming the mean birth weight in the every-other-day group, compared with the daily group, was no higher than the predefined noninferiority margin of 200 g (80% power and one-sided alpha of 0.05). Postrandomization characteristics, including blood glucose values and medication initiation and timing, were recorded. Satisfaction with treatment group was assessed using the validated Oxford Maternity Diabetes Treatment Satisfaction Questionnaire.

RESULTS:
A total of 197 patients were randomized: 98 in the daily group and 99 in the every-other-day group. Baseline characteristics were similar between groups. The mean neonatal birth weight was similar between groups (mean±SD 3,090±418 g among newborns in the daily group compared with 3,181±482 g among newborns in the every-other-day group). For the primary outcome, the every-other-day group was found to be noninferior to the daily group with an upper confidence limit for the mean difference in mean birth weight of 197 g, which was below the noninferiority margin of 200 g (P=.046). Postrandomization, there were no significant differences in the number of patients who required medication, the gestational age at which medication was started, or the type of medication used. Average fasting and postprandial glucose values were similar between groups. There was an increase in adherence to treatment group in those randomized to every-other-day blood sugars, but no difference in patient satisfaction.

CONCLUSION:
In patients with GDM, testing blood glucose values every other day was as effective as testing daily, without apparent effects on birth weight, medication initiation, or glucose control. Reduced frequency of blood glucose monitoring might help decrease the emotional, physical, and financial burden experienced by patients with GDM.

CLINICAL TRIAL REGISTRATION:

ClinicalTrials.gov, NCT04857073.
In Vitro Characterization of Fat Grafts Processed Using the REVOLVE ENVI System versus Decantation
Authors: Gabriel Allen , Kabaria Nimesh , Fang Carrie H. , Lombardi Jared A. , Stec Eric , Huang Li Ting , Li Hui , Sandor Maryellen 
Published: February 2024

Background:
This preclinical study evaluated benchtop/in vitro properties and fat viability and activity of grafts processed using the REVOLVE ENVI 600 system compared with decantation and evaluated properties of REVOLVE ENVI waste.

Methods:
Lipoaspirate from six donors was processed using REVOLVE ENVI or decantation. The composition of each graft, hematocrit/red blood cell content, fat particle size/macrostructure, viable adipocyte count, and adipocyte activity were analyzed. Stromal vascular fraction was analyzed for viable progenitor cell count and colony-forming units.

Results:
REVOLVE ENVI grafts had a higher mean (±SD) fat content at 85.6% ± 6.1% than decanted grafts at 72.1% ± 4.0% (P < 0.001), with negligible free oil (0.4% ± 1.1%) and cellular debris (<0.1%), whereas REVOLVE ENVI waste contained primarily aqueous fluid (91.0% ± 2.2%) with negligible viable fat. REVOLVE ENVI grafts had significantly lower hematocrit levels (P < 0.001) and contained significantly more large fat globules (P < 0.001) than decanted grafts or REVOLVE ENVI waste. The percentage of tissue particles of more than 1000 µm was highest for REVOLVE ENVI grafts at 61.6% ± 9.2% (decantation: 52.5% ± 13.4%; REVOLVE ENVI waste: 0.49% ± 1.50%), and the percentage of particles less than 200 µm was lowest for REVOLVE ENVI grafts at 15.7% ± 2.6% (decantation: 32.2% ± 8.9%; REVOLVE ENVI waste: 97.9% ± 4.5%). REVOLVE ENVI grafts contained 145.2% ± 36.0% more viable adipocytes, 145.7% ± 46.2% greater activity, 195.5% ± 104.2% more progenitors in SVF, and 363.5% ± 161.2% more SVF colony-forming units than decanted grafts.

Conclusion:
Fat grafts processed using REVOLVE ENVI demonstrated greater viability and activity than decanted grafts in vitro.
Endocarditis in a Community Teaching Hospital
Authors: Yang Ji Hyun , Tavares Liliana , Moon Soo Jin , Yoo Tae Kyung , Wagner Leonardo , Barbosa Felipe , Kinzfogl George , Chen Lucas , Treadwell Thomas 
Published: February 2024

Background
Endocarditis is a life-threatening condition with protean manifestations. The clinical syndrome continues to evolve with an aging population, the opioid epidemic, and changing microbiological profiles. Much of the clinical data for endocarditis comes from tertiary care centers with on-site cardiac surgery. Previous community cohorts do not reflect the current patient populations or current microbiology and predate the current opioid epidemic. We retrospectively reviewed the baseline clinical characteristics, microbiologic spectrum, surgical indication, tertiary referral, and outcomes for patients presenting to a community hospital with infective endocarditis (IE).

Methods
We retrospectively reviewed all adult medical patient admitted over a 5-year period with a clinical diagnosis of IE, from February 2016 to September 2021, to a single suburban safety net community hospital without on-site cardiac surgery.

Results
We identified 99 patients with a clinical diagnosis of IE. We observed a bimodal age distribution, with more comorbidities and predisposing cardiac conditions in the older patients and more intravenous drug use in the younger patients. Surgical indications were present in 44% of patients. Staphylococcus aureus was the most common pathogen. Overall survival was associated with fewer comorbidities and the absence of methicillin-resistant S. aureus infection. There was no difference in survival between patients with and without surgical indications.

Conclusions
IE continues to primarily affect older patients with comorbidities and results in a high 1-year mortality of 33%. A simple score including age, Charlson comorbidity index of 6.5, and methicillin-resistant S. aureus infection was predictive for in-patient, 30-day, and 1-year mortality.
Substantial decline of organ preservation fluid contamination following adoption of Ischemia-Free liver transplantation：a Post-hoc analysis
Authors: Lin Jianwen , Li Yefu , Fang Tongdi , Wang Tielong , Liao Kang , Zhao Qiang , Wang Dongping , Chen Maogen , Zhu Xiaofeng , Chen Yinghua , Chen Honghui , Guo Yiwen , Zhan Liqiang , Zhang Jiayi , Zhang Tao , Zeng Ping , Peng Yaqin , Yang Lu , Cai Changjie , Guo Zhiyong , He Xiaoshun 
Published: February 2024

Introduction:
Preservation fluid (PF) contaminations are common in conventional liver transplantation (CLT) and presumably originate from organ or PF exposures to the external environment in a non-strict sterile manner. Such exposures and PF contamination may be avoided in ischemia-free liver transplantation (IFLT) because of the strict sterile surgical procedures. In this study, we evaluated the impact of IFLT on organ PF contamination.

Methods:
A post-hoc analysis using data from the first randomized controlled trial of IFLT was performed to compare the incidence, pathogenic spectrum of PF contamination, and incidence of early recipient infection between IFLT and CLT. Multivariable logistic regression was used to explore risk factors for PF contamination.

Results:
Of the 68 cases recruited in the trial, 64 were included in this post-hoc analysis. The incidence of culture-positive PF was 9.4% (3/32) in the IFLT group versus 78.1% (25/32) in the CLT group (P<0.001). Three microorganisms were isolated from PF in the IFLT group, while 43 were isolated in the CLT group. The recipient infection rate within postoperative day 14 was 3.1% (1/32) in the IFLT group vs 15.6% (5/32) in the CLT group, although this difference did not reach statistical significance (P=0.196). Multivariate analysis revealed that adopting IFLT is an independent protective factor for culture-positive PF.

Conclusion:
PF contamination is substantially decreased in IFLT, and IFLT application is an independent protective factor for PF contamination. Using rigorous sterile measures and effective antibiotic therapy during IFLT may decrease PF contamination.
An Exploratory Analysis of Physical Examination Subtypes in Pediatric Athletes With Concussion
Authors: McPherson Jacob I. , Marsh Amanda C. , Cunningham Adam , Leddy John J. , Corrado Cathlyn , Cheema Zaheerud D. , Nazir Muhammad S. Z. , Nowak Andrew S. , Farooq Osman , Willer Barry S. , Haider Mohammad N. 
Published: February 2024

Objective:
Pediatric athletes with concussion present with a variety of impairments on clinical assessment and require individualized treatment. The Buffalo Concussion Physical Examination is a brief, pertinent clinical assessment for individuals with concussion. The purpose of this study was to identify physical examination subtypes in pediatric athletes with concussion within 2 weeks of injury that are relevant to diagnosis and treatment.

Design:
Secondary analysis of a published cohort study and clinician consensus.

Setting:
Three university-affiliated sports medicine centers.

Participants:
Two hundred seventy children (14.9 ± 1.9 years).

Independent Variables:
Orthostatic intolerance, horizontal and vertical saccades, smooth pursuits, vestibulo-ocular reflex, near-point convergence, complex tandem gait, neck range of motion, neck tenderness, and neck spasm.

Main Outcome Measures:
Correlations between independent variables were calculated, and network graphs were made. k-means and hierarchical clustering were used to identify clusters of impairments. Optimal number of clusters was assessed. Results were reviewed by experienced clinicians and consensus was reached on proposed subtypes.

Results:
Physical examination clusters overlapped with each other, and no optimal number of clusters was identified. Clinician consensus suggested 3 possible subtypes: (1) visio-vestibular (horizontal and vertical saccades, smooth pursuits, and vestibulo-ocular reflex), (2) cervicogenic (neck range of motion and spasm), and (3) autonomic/balance (orthostatic intolerance and complex tandem gait).

Conclusions:
Although we identified 3 physical examination subtypes, it seemed that physical examination findings alone are not enough to define subtypes that are both statistically supported and clinically relevant, likely because they do not include symptoms, assessment of mood or cognitive problems, or graded exertion testing.
Nutritional Status, Dietary Intake, Quality of Life and Dysphagia in Women With Fibromyalgia
Authors: Rodríguez María Correa , del Carmen Villaverde Rodríguez María , Barragán Antonio Casas , Haro Rosa María Tapia , Ferrándiz María Encarnación Aguilar 
Published: February 2024

Abstract

Background
Fibromyalgia syndrome (FMS) is an idiopathic chronic disease characterized by widespread musculoskeletal pain, hyperalgesia, and allodynia that has been recently associated with risk of dysphagia.

Objective
We aimed to analyze the association between nutritional status, micro- and macronutrient intake and quality of life (QoL) in a cohort of women with FMS and risk of dysphagia compared to women with FMS without risk of dysphagia.

Methods
A cross-sectional study was conducted in 46 women with FMS. Risk of dysphagia was assessed by the Eating Assessment Tool (EAT) and the Volume–Viscosity Test (V–VST). The Food Frequency Questionnaire and the Swallowing Quality of Life Questionnaire were used to assess dietary intake and QoL, respectively.

Results
Thirty women with FMS were at risk for dysphagia (65.21%) assessed by the EAT–10. Based on the V–VST, the frequency of risk of dysphagia was 63.04%. Significant differences in body mass index (BMI) were found between women at risk for dysphagia and those without risk. Women at risk for dysphagia had significantly lower overall QoL scores than those women without risk. No significant differences were found for dietary intake and dysphagia risk.

Discussion
Women with FMS at risk for dysphagia have significantly lower BMI values and worse QoL than women without dysphagia risk, supporting the importance of assessing dysphagia in clinical practice in persons with FMS.
Retrospective Validation of a Computerized Physiologic Equation to Predict Minute Ventilation Needs in Critically Ill Children*
Authors: Pelletier Jonathan H. , Rakkar Jaskaran , Au Alicia K. , Fuhrman Dana Y. , Clark Robert S.B. , Kochanek Patrick M. , Horvat Christopher M. 
Published: February 2024

OBJECTIVES:
Mechanical ventilation (MV) is pervasive among critically ill children. We sought to validate a computerized physiologic equation to predict minute ventilation requirements in children and test its performance against clinician actions in an in silico trial.

DESIGN:
Retrospective, electronic medical record linkage, cohort study.

SETTING:
Quaternary PICU.

PATIENTS:
Patients undergoing invasive MV, serial arterial blood gas (ABG) analysis within 1–6 hours, and pharmacologic neuromuscular blockade (NMB).

MEASUREMENTS AND MAIN RESULTS:
ABG values were filtered to those occurring during periods of NMB. Simultaneous ABG and minute ventilation data were linked to predict serial Paco
2 and pH values using previously published physiologic equations. There were 15,121 included ABGs across 500 encounters among 484 patients, with a median (interquartile range [IQR]) of 20 (10–43) ABGs per encounter at a duration of 3.6 (2.1–4.2) hours. The median (IQR) Paco
2 prediction error was 0.00 (–3.07 to 3.00) mm Hg. In Bland-Altman analysis, the mean error was –0.10 mm Hg (95% CI, –0.21 to 0.01 mm Hg). A nested, in silico trial of ABGs meeting criteria for weaning (respiratory alkalosis) or escalation (respiratory acidosis), compared the performance of recommended ventilator changes versus clinician decisions. There were 1,499 of 15,121 ABGs (9.9%) among 278 of 644 (43.2%) encounters included in the trial. Calculated predictions were favorable to clinician actions in 1124 of 1499 ABGs (75.0%), equivalent to clinician choices in 26 of 1499 ABGs (1.7%), and worse than clinician decisions in 349 of 1499 ABGs (23.3%). Calculated recommendations were favorable to clinician decisions in sensitivity analyses limiting respiratory rate, analyzing only when clinicians made changes, excluding asthma, and excluding acute respiratory distress syndrome.

CONCLUSIONS:
A computerized equation to predict minute ventilation requirements outperformed clinicians’ ventilator adjustments in 75% of ABGs from critically ill children in this retrospective analysis. Prospective validation studies are needed.