bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developmental loss of NMDA receptors results in supernumerary forebrain neurons through delayed maturation of transit-amplifying neuroblasts
Authors: Napoli Amalia J. , Laderwager Stephanie , Zoodsma Josiah D. , Biju Bismi , Mucollari Olgerta , Schubel Sarah K. , Aprea Christieann , Sayed Aaliya , Morgan Kiele , Napoli Annelysia , Flanagan Stephanie , Wollmuth Lonnie P. , Sirotkin Howard I. 
Published: February 2024

AbstractDevelopmental neurogenesis is a tightly regulated spatiotemporal process with its dysregulation implicated in neurodevelopmental disorders. NMDA receptors are glutamate-gated ion channels that are widely expressed in the early nervous system, yet their contribution to neurogenesis is poorly understood. Notably, a variety of mutations in genes encoding NMDA receptor subunits are associated with neurodevelopmental disorders. To rigorously define the role of NMDA receptors in developmental neurogenesis, we used a mutant zebrafish line (grin1−/−) that lacks all NMDA receptors yet survives to 10 days post-fertilization, offering the opportunity to study post-embryonic neurodevelopment in the absence of NMDA receptors. Focusing on the forebrain, we find that these fish have a progressive supernumerary neuron phenotype confined to the telencephalon at the end of embryonic neurogenesis, but which extends to all forebrain regions during postembryonic neurogenesis. This enhanced neuron population does not arise directly from increased numbers or mitotic activity of radial glia cells, the principal neural stem cells. Rather, it stems from a lack of timely maturation of transit-amplifying neuroblasts into post-mitotic neurons, as indicated by a decrease in expression of the ontogenetically-expressed chloride transporter, KCC2. Pharmacological blockade with MK-801 recapitulates the grin1−/− supernumerary neuron phenotype, indicating a requirement for ionotropic signaling. Thus, NMDA receptors are required for suppression of indirect, transit amplifying cell-driven neurogenesis by promoting maturational termination of mitosis. Loss of suppression results in neuronal overpopulation that can fundamentally change brain circuitry and may be a key factor in pathogenesis of neurodevelopmental disorders caused by NMDA receptor dysfunction.
Neuroimaging-based evidence for sympathetic correlation between brain activity and peripheral vasomotion during pain anticipation
Authors: Xu Ziqiang , Soh Zu , Kurota Yuta , Kimura Yuya , Hirano Harutoyo , Sasaoka Takafumi , Yoshino Atsuo , Tsuji Toshio 
Published: February 2024

AbstractAnticipation of pain engenders anxiety and fear, potentially shaping pain perception and governing bodily responses such as peripheral vasomotion through the sympathetic nervous system (SNS). Sympathetic innervation of vascular tone during pain perception has been quantified using a peripheral arterial stiffness index; however, its innervation role during pain anticipation remains unclear. This paper reports on a neuroimaging-based study designed to investigate the responsivity and attribution of the index at different levels of anticipatory anxiety and pain perception. The index was measured in a functional magnetic resonance imaging experiment that randomly combined three visual anticipation cues and painful stimuli of two intensities. The peripheral and cerebral responses to pain anticipation and perception were quantified to corroborate bodily responsivity, and their temporal correlation was also assessed to identify the response attribution of the index. Contrasting with the high responsivity across levels of pain sensation, a low responsivity of the index across levels of anticipatory anxiety revealed its specificity across pain experiences. Discrepancies between the effects of perception and anticipation were validated across regions and levels of brain activity, providing a brain basis for peripheral response specificity. The index was also characterized by a 1-s lag in both anticipation and perception of pain, implying top-down innervation of the periphery. Our findings suggest that the SNS responds to pain in an emotion-specific and sensation-unbiased manner, thus enabling an early assessment of individual pain perception using this index. This study integrates peripheral and cerebral hemodynamic responses toward a comprehensive understanding of bodily responses to pain.
Nrf2-mediated redox balance alleviates LPS-induced vascular endothelial cell inflammation by inhibiting endothelial cell ferroptosis
Authors: Hou Huimin , Qin Xiujiao , Li Gaokai , Cui Zhitao , Zhang Jin , Dong Bin , Wang Zhicheng , Zhao Huiying 
Published: February 2024

AbstractFerroptosis plays an important role in inflammation and oxidative stress. Whether ferroptosis is involved in the inflammation of vascular endothelial cells and its regulation mechanism remains unclear. We estimated the correlation between serum iron ion levels and the inflammation index of 33 patients with arteriosclerosis. In vitro, HUVECs with or without ferrostatin-1 were exposed to Lipopolysaccharide. Corresponding cell models to verify the target signaling pathway. The results showed that serum iron ion levels had a significant positive correlation with N ratio, N/L, LDL level, and LDL/HDL (P < 0.05), and a negative correlation with L ratio (P < 0.05) in the arteriosclerosis patients. In vitro, ferroptosis is involved in HUVECs inflammation. Ferrostatin-1 can rescue LPS-induced HUVECs inflammation by decreasing HMGB1/IL-6/TNF-α expression. Nrf2 high expression could protect HUVECs against ferroptosis by activating the GPX4/GSH system, inhibiting ferritinophagy, and alleviating inflammation in HUVECs by inhibiting HMGB1/IL-6/TNF-α expression. It also found that Nrf2 is a key adaptive regulatory factor in the oxidative damage of HUVECs induced by NOX4 activation. These findings indicated that ferroptosis contributed to the pathogenesis of vascular endothelial cell damage by mediating endothelial cell inflammation. Nrf2-mediated redox balance in vascular inflammation may be a therapeutic strategy in vascular diseases.
Investigating the genetic makeup of the major histocompatibility complex (MHC) in the United Arab Emirates population through next-generation sequencing
Authors: Marzouka Nour al dain , Alnaqbi Halima , Al-Aamri Amira , Tay Guan , Alsafar Habiba 
Published: February 2024

AbstractThe Human leukocyte antigen (HLA) molecules are central to immune response and have associations with the phenotypes of various diseases and induced drug toxicity. Further, the role of HLA molecules in presenting antigens significantly affects the transplantation outcome. The objective of this study was to examine the extent of the diversity of HLA alleles in the population of the United Arab Emirates (UAE) using Next-Generation Sequencing methodologies and encompassing a larger cohort of individuals. A cohort of 570 unrelated healthy citizens of the UAE volunteered to provide samples for Whole Genome Sequencing and Whole Exome Sequencing. The definition of the HLA alleles was achieved through the application of the bioinformatics tools, HLA-LA and xHLA. Subsequently, the findings from this study were compared with other local and international datasets. A broad range of HLA alleles in the UAE population, of which some were previously unreported, was identified. A comparison with other populations confirmed the current population’s unique intertwined genetic heritage while highlighting similarities with populations from the Middle East region. Some disease-associated HLA alleles were detected at a frequency of > 5%, such as HLA-B*51:01, HLA-DRB1*03:01, HLA-DRB1*15:01, and HLA-DQB1*02:01. The increase in allele homozygosity, especially for HLA class I genes, was identified in samples with a higher level of genome-wide homozygosity. This highlights a possible effect of consanguinity on the HLA homozygosity. The HLA allele distribution in the UAE population showcases a unique profile, underscoring the need for tailored databases for traditional activities such as unrelated transplant matching and for newer initiatives in precision medicine based on specific populations. This research is part of a concerted effort to improve the knowledge base, particularly in the fields of transplant medicine and investigating disease associations as well as in understanding human migration patterns within the Arabian Peninsula and surrounding regions.
High brightness and low operating voltage CsPbBr3 perovskite LEDs by single-source vapor deposition
Authors: Yeh Kuan-Chi , Chan Chia-Hua 
Published: February 2024

AbstractIn this work, we utilized CsPbBr3 powder as the precursor material for the single-source vapor deposition (SSVD) process to fabricate the CsPbBr3 emitting layer. Due to the high density of grain boundaries and defects in the thin films deposited in the initial stages, non-radiative recombination can occur, reducing the efficiency of perovskite light-emitting diodes (PeLED). To address this issue, we employed a thermal annealing process by subjecting the perovskite films to the appropriate annealing temperature, facilitating the coalescence and growth of different grains, improving lattice integrity, and thereby reducing the presence of defects and enhancing the photoluminescence performance of the films. Furthermore, in this study, we successfully fabricated simple-structured CsPbBr3 PeLED using thermally annealed CsPbBr3 films. Among these components, even without adding the electron and hole transport layers, the best-performing device achieved a maximum brightness of 14,079 cd/m2 at a driving voltage of only 2.92 V after annealing at 350 °C; the brightness is 16.8 times higher than that of CsPbBr3 PeLED without heat treatment, demonstrating outstanding light-emitting performance. The research results show that using SSVD to prepare CsPbBr3 PeLED has broad application potential, providing a simple process option for research on improving the performance of PeLED.
Immunological characterization of a long-lasting response in a patient with metastatic triple-negative breast cancer treated with PD-1 and LAG-3 blockade
Authors: Rivoltini Licia , Camisaschi Chiara , Fucà Giovanni , Paolini Biagio , Vergani Barbara , Beretta Valeria , Damian Silvia , Duca Matteo , Cresta Sara , Magni Michele , Leone Biagio Eugenio , Castelli Chiara , de Braud Filippo , De Santis Francesca , Di Nicola Massimo 
Published: February 2024

AbstractIn patients with advanced triple-negative breast cancer (TNBC), translational research efforts are needed to improve the clinical efficacy of immunotherapy with checkpoint inhibitors. Here, we report on the immunological characterization of an exceptional, long-lasting, tumor complete response in a patient with metastatic TNBC treated with dual PD-1 and LAG-3 blockade within the phase I/II study CLAG525X2101C (NCT02460224) The pre-treatment tumor biopsy revealed the presence of a CD3+ and CD8+ cell infiltrate, with few PD1+ cells, rare CD4+ cells, and an absence of both NK cells and LAG3 expression. Conversely, tumor cells exhibited positive staining for the three primary LAG-3 ligands (HLA-DR, FGL-1, and galectin-3), while being negative for PD-L1. In peripheral blood, baseline expression of LAG-3 and PD-1 was observed in circulating immune cells. Following treatment initiation, there was a rapid increase in proliferating granzyme-B+ NK and T cells, including CD4+ T cells, alongside a reduction in myeloid-derived suppressor cells. The role of LAG-3 expression on circulating NK cells, as well as the expression of LAG-3 ligands on tumor cells and the early modulation of circulating cytotoxic CD4+ T cells warrant further investigation as exploitable predictive biomarkers for dual PD-1 and LAG-3 blockade.Trial registration: NCT02460224. Registered 02/06/2015.
Ready-to-transfer two-dimensional materials using tunable adhesive force tapes
Authors: Nakatani Maki , Fukamachi Satoru , Solís-Fernández Pablo , Honda Satoshi , Kawahara Kenji , Tsuji Yuta , Sumiya Yosuke , Kuroki Mai , Li Kou , Liu Qiunan , Lin Yung-Chang , Uchida Aika , Oyama Shun , Ji Hyun Goo , Okada Kenichi , Suenaga Kazu , Kawano Yukio , Yoshizawa Kazunari , Yasui Atsushi , Ago Hiroki 
Published: February 2024

AbstractGraphene and other two-dimensional (2D) materials can be used to create electronic and optoelectronic devices. However, their development has been limited by the lack of effective large-area transfer processes. Here we report a transfer method that uses functional tapes with adhesive forces controlled by ultraviolet light. The adhesion of the tape is optimized for the transfer of monolayer graphene, providing a yield of over 99%. Once detached from the growth substrate, the graphene/tape stack enables easy transfer of graphene to the desired target substrate. The method can be used to transfer other 2D materials, including bilayer graphene, transition metal dichalcogenides, hexagonal boron nitride and stacked heterostructures. The solvent-free nature of the final release step facilitates transfer to various target substrates including flexible polymers, paper and three-dimensional surfaces. The tape/2D material stacks can also be cut into desired sizes and shapes, allowing site-selective device fabrication with reduced loss of 2D materials.
Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling
Authors: Zhang Yuqing , Young Rose , Garvanska Dimitriya H. , Song Chunlin , Zhai Yujing , Wang Ying , Jiang Hongfei , Fang Jing , Nilsson Jakob , Alfieri Claudio , Zhang Gang 
Published: February 2024

AbstractAccurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi). Here we generated Cdc20 RNAi-sensitive cell lines which display a penetrant metaphase arrest by a single RNAi treatment. In this null background, we accurately measured the contribution of each known motif of Cdc20 on APC/C and SAC activation. The CRY box, a previously identified degron, was found critical for SAC by promoting MCC formation and its interaction with APC/C. These data reveal additional regulation within the SAC and establish a novel method to interrogate Cdc20.
Mechanistic model of radiotherapy-induced lung fibrosis using coupled 3D agent-based and Monte Carlo simulations
Authors: Cogno Nicolò , Bauer Roman , Durante Marco 
Published: February 2024

Abstract
Background
Mechanistic modelling of normal tissue toxicities is unfolding as an alternative to the phenomenological normal tissue complication probability models. The latter, currently used in the clinics, rely exclusively on limited patient data and neglect spatial dose distribution information. Among the various approaches, agent-based models are appealing as they provide the means to include patient-specific parameters and simulate long-term effects in complex systems. However, Monte Carlo tools remain the state-of-the-art for modelling radiation transport and provide measurements of the delivered dose with unmatched precision.

Methods
In this work, we develop and characterize a coupled 3D agent-based – Monte Carlo model that mechanistically simulates the onset of the radiation-induced lung fibrosis in an alveolar segment. To the best of our knowledge, this is the first such model.

Results
Our model replicates extracellular matrix patterns, radiation-induced lung fibrosis severity indexes and functional subunits survivals that show qualitative agreement with experimental studies and are consistent with our past results. Moreover, in accordance with experimental results, higher functional subunits survival and lower radiation-induced lung fibrosis severity indexes are achieved when a 5-fractions treatment is simulated. Finally, the model shows increased sensitivity to more uniform protons dose distributions with respect to more heterogeneous ones from photon irradiation.

Conclusions
This study lays thus the groundwork for further investigating the effects of different radiotherapeutic treatments on the onset of radiation-induced lung fibrosis via mechanistic modelling.
Deep learning-based optical coherence tomography angiography image construction using spatial vascular connectivity network
Authors: Le David , Son Taeyoon , Kim Tae-Hoon , Adejumo Tobiloba , Abtahi Mansour , Ahmed Shaiban , Rossi Alfa , Ebrahimi Behrouz , Dadzie Albert , Ma Guangying , Lim Jennifer I. , Yao Xincheng 
Published: February 2024

AbstractOptical coherence tomography angiography (OCTA) provides unrivaled capability for depth-resolved visualization of retinal vasculature at the microcapillary level resolution. For OCTA image construction, repeated OCT scans from one location are required to identify blood vessels with active blood flow. The requirement for multi-scan-volumetric OCT can reduce OCTA imaging speed, which will induce eye movements and limit the image field-of-view. In principle, the blood flow should also affect the reflectance brightness profile along the vessel direction in a single-scan-volumetric OCT. Here we report a spatial vascular connectivity network (SVC-Net) for deep learning OCTA construction from single-scan-volumetric OCT. We quantitatively determine the optimal number of neighboring B-scans as image input, we compare the effects of neighboring B-scans to single B-scan input models, and we explore different loss functions for optimization of SVC-Net. This approach can improve the clinical implementation of OCTA by improving transverse image resolution or increasing the field-of-view.
Mitochondrial outer membrane integrity regulates a ubiquitin-dependent and NF-κB-mediated inflammatory response
Authors: Vringer Esmee , Heilig Rosalie , Riley Joel S , Black Annabel , Cloix Catherine , Skalka George , Montes-Gómez Alfredo E , Aguado Aurore , Lilla Sergio , Walczak Henning , Gyrd-Hansen Mads , Murphy Daniel J , Huang Danny T , Zanivan Sara , Tait Stephen WG 
Published: February 2024

AbstractMitochondrial outer membrane permeabilisation (MOMP) is often essential for apoptosis, by enabling cytochrome c release that leads to caspase activation and rapid cell death. Recently, MOMP has been shown to be inherently pro-inflammatory with emerging cellular roles, including its ability to elicit anti-tumour immunity. Nonetheless, how MOMP triggers inflammation and how the cell regulates this remains poorly defined. We find that upon MOMP, many proteins localised either to inner or outer mitochondrial membranes are ubiquitylated in a promiscuous manner. This extensive ubiquitylation serves to recruit the essential adaptor molecule NEMO, leading to the activation of pro-inflammatory NF-κB signalling. We show that disruption of mitochondrial outer membrane integrity through different means leads to the engagement of a similar pro-inflammatory signalling platform. Therefore, mitochondrial integrity directly controls inflammation, such that permeabilised mitochondria initiate NF-κB signalling.
Koopman fault‐tolerant model predictive control
Authors: Bakhtiaridoust Mohammadhosein , Yadegar Meysam , Jahangiri Fatemeh 
Published: February 2024

AbstractThis paper introduces a novel data‐driven approach to develop a fault‐tolerant model predictive controller (MPC) for non‐linear systems. By adopting a Koopman operator‐theoretic perspective, the proposed method leverages historical data from the system to construct a data‐driven model that captures the non‐linear behaviour and fault characteristics. The fault influence is addressed through an online estimation of a time‐varying Koopman predictor, which allows for adjusting the MPC control law to counteract the fault effects. This estimation is performed in a higher dimensional Koopman feature space, where the dynamics behave linearly. As a result, the non‐linear fault‐tolerant MPC optimization problem can be replaced with a more practical and feasible linear time‐varying one using the approximated Koopman predictor. Moreover, by incorporating the online update procedure, the time‐varying Koopman predictor can represent the dynamics of the faulty system. Hence, the controller can adapt and compensate for the faults in real‐time, integrating the fault diagnosis module in the MPC framework and eliminating the need for a separate fault detection unit. Finally, the efficacy of the proposed approach is demonstrated through case study results, which highlight the ability of the controller to mitigate faults and maintain desired system behaviour.
Design and development of robotic system for precise vascular access and autonomous venipuncture
Authors: Kim Seho , Choi Seonil , Yeom Kiwon 
Published: February 2024

AbstractThe medical robot systems have the effect of reducing the work load and preventing accidents by supporting task that are difficult for inexperienced medical practitioners. Recently, research on automated blood collection and injection robotic system has been increasing, but work is still necessary such as replacing needle tips, setting tourniquet, and fixing the patient's arm. This letter proposes an intelligent intravenous injection robot without having to see the medical workers face‐to‐face. A revolving system is designed for automatic needle replacement and an arm holder for vessel fixation. Finally, positioning the needle at the target point is experimented by position control based on mathematical modelling and velocity kinematics.