bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stress-induced c-fos expression in the medial prefrontal cortex of male rats differentially involves the main glial cell phenotypes
Authors: Aguilar-Delgadillo Adriana , Cruz-Mendoza Fernando , Luquin-de Anda Sonia , Ruvalcaba-Delgadillo Yaveth , Jáuregui-Huerta Fernando 
Published: December 2023

AbstractStress poses a challenge to the body’s equilibrium and triggers a series of responses that enable organisms to adapt to stressful stimuli. The prefrontal cortex (PFC), particularly in acute stress conditions, undergoes significant physiological changes to cope with the demands associated with cellular activation. The proto-oncogene c-fos and its protein product c-Fos have long been utilized to investigate the effects of external factors on the central nervous system (CNS). While c-Fos expression has traditionally been attributed to neurons, emerging evidence suggests its potential expression in glial cells. In this study, our main objective was to explore the expression of c-Fos in glial cells and examine how acute stress influences these activity patterns. We conducted our experiments on male Wistar rats, subjecting them to acute stress and sacrificing them two hours after the stressor initiation. Using double-labelling fluorescent immunohistochemistry targeting c-Fos, along with markers such as GFAP, Iba-1, Olig2, NG2, and NeuN, we analyzed a series of 35 μm brain slices obtained from the medial PFC. Our findings compellingly demonstrate that c-Fos expression extends beyond neurons and is present in astrocytes, oligodendrocytes, microglia, and NG2 cells—the diverse population of glial cells. Moreover, we observed distinct regulation of c-Fos expression in response to stress across the different phenotypes. These results emphasize the importance of considering glial cells and their perspective in studies investigating brain activity, highlighting c-Fos as a response marker in glial cells. By shedding light on the differential regulation of c-Fos expression in response to stress, our study also contributes to the understanding of glial cell involvement in stress-related processes.
Personalized Pangenome References
Authors: Sirén Jouni , Eskandar Parsa , Ungaro Matteo Tommaso , Hickey Glenn , Eizenga Jordan M. , Novak Adam M. , Chang Xian , Chang Pi-Chuan , Kolmogorov Mikhail , Carroll Andrew , Monlong Jean , Paten Benedict 
Published: December 2023

AbstractPangenomes, by including genetic diversity, should reduce reference bias by better representing new samples compared to them. Yet when comparing a new sample to a pangenome, variants in the pangenome that are not part of the sample can be misleading, for example, causing false read mappings. These irrelevant variants are generally rarer in terms of allele frequency, and have previously been dealt with using allele frequency filters. However, this is a blunt heuristic that both fails to remove some irrelevant variants and removes many relevant variants. We propose a new approach, inspired by local ancestry inference methods, that imputes a personalized pangenome subgraph based on sampling local haplotypes according tok-mer counts in the reads. Our approach is tailored for the Giraffe short read aligner, as the indexes it needs for read mapping can be built quickly. We compare the accuracy of our approach to state-of-the-art methods using graphs from the Human Pangenome Reference Consortium. The resulting personalized pangenome pipelines provide faster pangenome read mapping than comparable pipelines that use a linear reference, reduce small variant genotyping errors by 4x relative to the Genome Analysis Toolkit (GATK) best-practice pipeline, and for the first time make short-read structural variant genotyping competitive with long-read discovery methods.
Phylogenomic Insights into the Evolution and Origin of Nematoda
Authors: Qing Xue , Zhang Y. Miles , Sun Sidi , Ahmed Mohammed , Lo Wen-Sui , Bert Wim , Holovachov Oleksandr , Li Hongmei 
Published: December 2023

AbstractThe phylum Nematoda represents one of the most cosmopolitan and abundant metazoan groups on Earth. In this study, we reconstructed the phylogenomic tree for phylum Nematoda. A total of 60 genomes, belonging to eight nematode orders, were newly sequenced, providing the first low-coverage genomes for the orders Dorylaimida, Mononchida, Monhysterida, Chromadorida, Triplonchida, and Enoplida. The resulting phylogeny is well-resolved across most clades, with topologies remaining consistent across various reconstruction parameters. The subclass Enoplia is placed as a sister group to the rest of Nematoda, agrees with previous published phylogenies. While the order Triplonchida is monophyletic, it is not well-supported, and the order Enoplida is paraphyletic. Taxa possessing a stomatostylet form a monophyletic group; however, the superfamily Aphelenchoidea does not constitute a monophyletic clade. The generaTrichinellaandTrichurisare inferred to have shared a common ancestor approximately 202 mya, a considerably later period than previously suggested. All stomatostylet-bearing nematodes are proposed to have originated ∼305 mya, corresponding to the transition from the Devonian to the Permian period. The genusThorniais placed outside of Dorylaimina and Nygolaimina, disagreeing with its position in previous studies. Additionally, we tested the whole genome amplification method and demonstrated that it is a promising strategy for obtaining sufficient DNA for phylogenomic studies of microscopic eukaryotes. This study significantly expanded the current nematode genome dataset, and the well-resolved phylogeny enhances our understanding of the evolution of Nematoda.
Cholesterol modulates the human FPN1 iron export function in plasma membrane liquid-ordered microdomains
Authors: Debbiche Rim , Ka Chandran , Gourlaouen Isabelle , Maestri Sandrine , Uguen Kévin , Jaffrès Paul-Alain , Callebaut Isabelle , Le Gac Gérald 
Published: December 2023

ABSTRACTFerroportin 1 (FPN1) is the only known mammalian iron efflux transporter. This multi-pass membrane protein, which adopts the Major Facilitator Superfamily fold, is tightly controlled by serum hepcidin to assure maintenance of adequate cellular and systemic iron levels. Earlier studies have shown that cholesterol-lowering drugs can reduce FPN1 expression in liquid-ordered plasma membrane microdomains and its sensitivity to hepcidin. However, the molecular mechanism by which cholesterol depletion regulates the localization of FPN1 at the cell surface remains unknown. In biochemical experiments, we show that cholesterol depletion reduces the iron export function of FPN1. Repletion with cholesterol restores FPN1 activity. This is not observed with the diastereoisomer epicholesterol, suggesting a direct interaction between cholesterol and FPN1. Consistent with this, we demonstrate that mutants affecting the key tyrosine residues of three cholesterol-recognition amino acid consensus (CRAC/CARC) motifs have a negative impact on FPN1 activity, in manner that also decreases its abundance in ordered plasma membrane microdomains. A complementary structural analysis allows us to focus on a conserved CARC motif (CARC-1) located in a deep hydrophobic groove between transmembrane helices 1 and 5. Molecular docking suggests that this groove is well suited to cholesterol binding. All these findings indicate that the interaction between FPN1 and cholesterol is of major importance for the localization of FPN1 in ordered microdomains of the plasma membrane, which is necessary for its optimal activity, and so its responsiveness to hepcidin.
Performance analysis of electrical environmental control and ice protection systems in a commercial transport aircraft
Authors: Ercan Hamdi , Öztürk Cüneyt , Akın Mustafa 
Published: December 2023

Purpose
This paper aims to assess the impact of electrifying the environmental control system (ECS) and ice protection system (IPS), the primary pneumatic system consumers in a conventional commercial transport aircraft, on aircraft weight, range, and fuel consumption.

Design/methodology/approach
The case study was carried out on Airbus A321-200 aircraft. Design, modelling and analysis processes were carried out on Pacelab SysArc software. Conventional and electrical ECS and IPS architectures were modelled and analysed considering different temperature profiles.

Findings
The simulation results have shown that the aircraft model with ±270 VDC ECS and IPS architecture is lighter, has a more extended range and has less relative fuel consumption. In addition, the simulation results showed that the maximum range and relative fuel economy of all three aircraft models increased slightly as the temperature increased.

Practical implications
Considering the findings in this paper, it is seen that the electrification of the conventional pneumatic system in aircraft has positive contributions in terms of weight, power consumption and fuel consumption.

Social implications
The positive contributions in terms of weight, power consumption and fuel consumption in aircraft will be direct environmental and economic contributions.

Originality/value
Apart from the conventional ECS and IPS of the aircraft, two electrical architectures, 230 VAC and ±270 VDC, were modelled and analysed. To see the effects of the three models created in different temperature profiles, analyses were done for cold day, ISA standard day and hot day temperature profiles.
Hybrid business offerings in small internationalisers: a mixed-method analysis of internal capabilities through hesitant fuzzy information
Authors: Amoozad Mahdiraji Hannan , Abbasi Kamardi Aliasghar , Jafari-Sadeghi Vahid , Razavi Hajiagha Seyed Hossein , Castellano Sylvaine 
Published: December 2023

PurposeIn this research, the initial list of internal capabilities in small and medium-sized enterprises (SMEs) leading to success in international markets has been extracted. Then, the most relevant capabilities to international SMEs under servitisation and hybrid offerings have been screened. Next, the selected capabilities have been classified, and ultimately the relationship amongst the capabilities has been analysed. The conceptual model for SMEs participating in international markets with hybrid offerings has been illustrated.Design/methodology/approachA literature review has been employed to extract the initial list of internal capabilities to address the research objectives. Then, a novel hesitant fuzzy Delphi (HFD) method has been developed to select the most relevant capabilities for SMEs for hybrid offerings in international markets by using the experts opinions. Subsequently, a novel hesitant fuzzy interpretive structural modelling (HFISM) has been developed to classify the capabilities, design a level-based conceptual model and present the relationship amongst the prominent capabilities.FindingsAfter the literature review, sixteen internal capabilities leading to success in the international market via hybrid offerings have been extracted. Then, eight selected capabilities were chosen for further investigation by applying 15 expert opinions and via the HFD approach. According to HFISM results, a level-based conceptual model was emanated, and “ability to take advantage of international opportunities”, “financial strength”, “technology level” and “efficient innovation management” were considered as the most fundamental capabilities resulting in successful hybrid offerings in international markets.Originality/valueAlongside the multi-layer decision-making approach developed in this manuscript to analyse the internal capabilities roles in hybrid offering success towards international markets, to the best knowledge of the authors, the hesitant fuzzy approaches developed in this article have not been previously presented by any other scholar. A novel HFD approach has been designed for consensus amongst the experts under uncertain circumstances. Furthermore, a novel HFISM has been suggested and employed in this research to comprehend the relationship amongst the internal capabilities.
Sustainable development goals in the hospitality industry: a dream or reality?
Authors: Ferrero-Ferrero Idoya , Muñoz-Torres María Jesús , Rivera-Lirio Juana María , Escrig-Olmedo Elena , Fernández-Izquierdo María Ángeles 
Published: December 2023

Purpose
The purpose of this paper is to explore how effectively leading sustainable hotels have integrated Sustainable Development Goals (SDGs) into their reporting. The main aim is to pinpoint areas for improvement concerning SDG reporting which can help the hospitality industry to achieve a transformation in a more SDG-aligned global tourism system.

Design/methodology/approach
For this study, a content analysis technique was used to extract the information regarding strategic consistency of SDG reporting. Both qualitative and quantitative approaches were applied to the analysis of this information. This paper seeks to assess the extent to which the materiality analysis, corporate targets and performance indicators defined by the world’s top sustainable hotels in their sustainability reports are consistent with those SDGs linked to the business. To that end, the authors have selected the most sustainable hotels according to the SAM Corporate Sustainability Assessment in 2020.

Findings
The results of this study show that the most sustainable hotel companies did not take a strategic consistency approach when reporting the SDGs. These findings identify four areas for improvement concerning reporting, which may promote the adoption of a strategic and consistent approach in SDG reporting.

Practical implications
This study includes a set of recommendations to provide the market with complete, coherent and comparable information on their contribution to the SDGs and, therefore, foster collective learning to bring about sustainable tourism transformation.

Originality/value
This paper represents a contribution to the discussion on the strategic or symbolic implementation of SDGs at a corporate level. In addition, this paper reflects a deeper understanding of how hotel companies could improve their reporting and management system to contribute to SDGs.
Analysis of trends that turn an entrepreneurship idea into a family business: an article in perspective
Authors: Areiza-Padilla Jose Andres , Galindo-Becerra Tatiana , Veas-González Iván , Barajas-Portas Karla 
Published: December 2023

PurposeThis article examines some of the trends that allow to understand and analyze the evolution of the idea of entrepreneurship to become a family business.Design/methodology/approachThis paper is based on systematic research.FindingsAround four current trends and four future trends are presented, which allow the authors to understand how the family of an entrepreneur influences in a direct and indirect way in their business, until even managing to transform that business into a family business through planning, organization, management and control exercised by several members of the family of the initial entrepreneur and his future generations in that company.Originality/valueThis research makes it possible to identify some challenges and opportunities that family businesses must face, which arise from an enterprise and which can help them to have business success, covering part of the past, present and future of such organizations. In this way, this article synthesizes how family dynamics and business dynamics are intertwined through the influence of the family on an entrepreneur’s business model.
Contemporaneous causality among office property prices of major Chinese cities with vector error correction modeling and directed acyclic graphs
Authors: Xu Xiaojie , Zhang Yun 
Published: December 2023

Purpose
This study aims to investigate dynamic relations among office property price indices of 10 major cities in China for the years 2005–2021.

Design/methodology/approach
Using monthly data, the authors adopt vector error correction modeling and the directed acyclic graph for the characterization of contemporaneous causality among the 10 indices.

Findings
The PC algorithm identifies the causal pattern, and the linear non-Gaussian acyclic model algorithm further determines the causal path from which we perform innovation accounting analysis. Sophisticated price dynamics are found in price adjustment processes following price shocks, which are generally dominated by the top tier of cities.

Originality/value
This suggests that policies on office property prices, in the long run, might need to be planned with particular attention paid to the top tier of cities.
Rules or capacity for the EU’s fiscal future? From Werner and McDougall to the present
Authors: Georgiou Christakis 
Published: December 2023

PurposeThe COVID19 crisis has thrown wide open the debate on Europe’s Economic and Monetary Union’s (EMU) future. Next Generation EU (NGEU) has broken the stalemate over a central fiscal capacity. The open question is whether NGEU is a one-off or a first step. The suspension of the Stability and Growth Pact has given new urgency to the debate on reforming EMU’s fiscal rules.Design/methodology/approachThere is no debate as yet about how these two prospects relate to each other. This paper argues that a permanent fiscal capacity and revised rules should be seen as alternatives.FindingsThis study makes two claims: first, a fiscal capacity renders a reformed pact unnecessary and second, that is an optimal solution politically. A fiscal capacity would provide an efficient asymmetric shock absorber and therefore reduce the need for pre-emptive action against negative cross-border externalities. It would also provide an abundant supply of an EU-wide safe asset around which to structure the EU’s financial system, thus rendering unnecessary the backstopping of member states' debts.Originality/valueThis would restore democratic accountability while eliminating moral hazard and enforcement problems.
Optimization of the printability envelope of low-viscosity powder-binder feedstocks used in material extrusion 3D printing
Authors: Omrane Mohamed Ahmed , Côté Raphaël , Demers Vincent 
Published: December 2023

Purpose
The purpose of this study is to determine the material extrusion (MEX) printability envelope of a new kind of low-viscosity powder-binder feedstocks using rheological properties.

Design/methodology/approach
Formulation of 13 feedstocks (variation of solid loading 60–67 Vol.% and thickening agent proportion 3–15 Vol.%) that were characterized and printed at different temperatures.

Findings
Three rheological models were successfully used to define the viscosity envelope, producing stable and defect-free printing. At a shear deformation rate experienced by the feedstock in the nozzle ranging from 100 to 300 s–1, it was confirmed that metal injection molding (MIM) feedstocks exhibiting a low viscosity between 100 and 150 Pa s could be printed using an extrusion temperature as low as 85 °C.

Practical implications
MEX can be used in synergy with MIM to accelerate mold development for a new injected part or simply as a replacement for MIM when the cost of the mold becomes too high for very small production volumes.

Originality/value
Correlation between the rheological properties of this new generation of low-viscosity feedstocks and MEX printability has been demonstrated for the first time.
Going green and staying loyal: how different are generation Z travellers towards green brand loyalty of hotels
Authors: Rasheed A.K. Fazeen , Balakrishnan Janarthanan 
Published: December 2023

Purpose
This study aims to examine the influence of collectivism, brand prestige and brand identification on green brand loyalty among Generation Z and older generation travellers in India. Loyal consumers are essential for the long-term success of hotels.

Design/methodology/approach
A single cross-sectional design is used in the study, with a total of 277 Generation Z and 299 Older generation travellers surveyed directly at three different tourist destinations in India. The PLS-SEM approach is used to analyse the data.

Findings
The study’s findings showed that among Generation Z travellers, factors such as collectivism, brand identification and brand prestige play a significant role in shaping green brand loyalty towards hotels. Furthermore, we noted that collectivism directly influences brand prestige and identification. Conversely, for the older generation, the study did not substantiate the impacts of collectivism on green brand loyalty nor the relationship between brand identification and green brand loyalty.

Originality/value
This study uniquely contributes to the literature by revealing generational differences in the influence of collectivism and brand factors on green brand loyalty within the Indian hotel industry.
Applying genomic approaches to identify historic population declines in European forest bats
Authors: Razgour Orly , Montauban Cecilia , Festa Francesca , Whitby Daniel , Juste Javier , Ibáñez Carlos , Rebelo Hugo , Afonso Sandra , Bekaert Michael , Jones Gareth , Williams Carol , Boughey Katherine 
Published: December 2023

Abstract

Anthropogenically driven environmental changes over recent centuries have led to severe declines of wildlife populations. Better tools are needed to assess the magnitude and consequences of these declines. Anecdotal evidence suggests European bat populations have suffered substantial declines in the past centuries. However, there is little empirical evidence of these declines that can be used to put more recent population trends into historic context.

This study is a collaboration between academics and conservation practitioners to develop molecular approaches capable of providing evidence of historic population changes that can inform conservation status assessments and management. We generated a genomic dataset of 46,872 SNPs for the Western barbastelle, Barbastella barbastellus, a regionally Vulnerable bat species, including colonies from across the species' British and Iberian ranges. We used a combination of landscape genetics and model‐based inference of demographic history to identify both evidence of population size changes and possible drivers of these changes.

Levels of genetic diversity increased and inbreeding decreased with increasing broadleaf woodland cover around the colony. Genetic connectivity was impeded by artificial lights and facilitated by rivers and broadleaf woodland cover.

The demographic history analysis showed that both the northern and southern British barbastelle populations have declined by 99% over the past 330–548 years. These declines may be linked to the loss of large oak trees and native woodlands due to shipbuilding during the early colonial period.

Synthesis and applications. Genomic approaches can provide a better understanding of the conservation status of threatened species, within historic and contemporary contexts, and inform their conservation management. Our findings of will directly influence the definition of the Favourable Conservation Status of the barbastelle, in turn influencing considerations of the conservation of the species in development plans. Knowledge gained will also help set species recovery targets. Policymakers are interested in using our approach for other species. This study shows how we can bridge the implementation gap between genomic research and direct conservation applications. There is an urgent need to carry out such collaborative studies for other priority species to enable informed species recovery interventions via policy mechanisms and project delivery.