bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of novel LAMP and qPCR assays for rapid and specific identification of Bronze birch borer (Agrilus anxius)
Authors: Peterson Donnie L. , Pecori Francesco , Luchi Nicola , Migliorini Duccio , Santini Alberto , Kyle Kathleen E. , Rutledge Claire , Sallé Aurélien , Kaya Sezer Olivia , Ramsfield Tod , Cleary Michelle 
Published: December 2023

AbstractBuprestids are an emerging threat to broadleaf forests across the world. Bronze birch borer (Agrilus anxius, BBB) poses a serious threat to European birch species if the insect were to be introduced. Due to their cryptic lifestyle feeding on the vascular tissue of their host plants, buprestids and other wood borers can be difficult to observe or detect. Early detection tools are vital to swiftly implement eradication measures and prevent the establishment of introduced species. In this study, we developed novel qPCR and LAMP assays for BBB and investigated the specificity and sensitivity for their use as early detection tools in European forests. Plant chemicals may limit these assays, so we conducted sensitivity testing with extracted foliage and plant vascular tissues to determine potential inhibition effects on DNA amplification. Both assays were specific to the target species when tested against the DNA of 17 other European Agrilus/buprestid species, two Scolytinae, and five Cerambycids (N = 24). Both assays varied in sensitivity with the qPCR assay amplifying at a concentration as low as 20 fg/μL, whereas the LAMP assay amplified as low as 3.2 pg/μL. Plant chemicals in DNA extracts from leaves did not impact the sensitivity of either assay, reaching similar detection levels. In contrast, vascular tissue reduced the sensitivity of the LAMP assay, amplifying as low as 0.04 ng/μL compared with 0.008 ng/μL in the control. These results demonstrate that both assays are highly specific and sensitive tools that can be used to detect frass and identify larvae as well as monitor the spread of A. anxius. qPCR resulted in more sensitive than LAMP overall. Thus, if results are needed quickly to make fast management decisions or as an initial screening of samples, the LAMP method is optimal. However, if fine detection is critical, then qPCR is preferential.
Trauma‐informed interactions within a trauma‐informed homeless service provider: Staff and client perspectives
Authors: Barry Amanda R. , Hoffman Erin , Martinez‐Charleston Elizabeth , DeMario Milena , Stewart Justine , Mohiuddin Madeeha , Mihelicova Martina , Brown Molly 
Published: December 2023

AbstractThis study aims to provide practical insights for developing trauma‐informed interaction strategies between service providers and participants in homeless service organizations (HSOs). Twelve providers and 17 participants were interviewed for a qualitative study on trauma‐informed care (TIC) within an HSO. Thematic analysis revealed six themes regarding provider approaches to TIC interactions with participants: provide a sounding board, promote safety, foster understanding and respect, build relationships and trust, facilitate connection to services, and ensure flexibility in service provision. Participants noted three themes regarding their views of TIC interactions with providers: possess education and experience, build relationships and trust, and demonstrate supportive interpersonal styles. This study describes the application of TIC within an HSO, emphasizing the importance of supportive, positive interactions that promote understanding, respect, and trust. It highlights key factors in service provision. The findings expand our understanding of TIC implementation in HSOs and suggest areas for improvement.
Recent developments in heterologous production of cellulases and xylanases using the Pichia pastoris expression system
Authors: Ullah Hayat , Madadi Meysam , Asadollahi Mohammad Ali , Hu Yun , Dou Shaohua , Yan Junshu , Huan Hailin , Sun Fubao 
Published: December 2023

AbstractThe yeast expression system, Pichia pastoris, is one of the most robust and versatile expression systems in biotechnology and molecular biology, generally considered as a safe host for heterologous protein expression, especially for producing cellulases and xylanases at the industrial level. Despite the high recombinant protein expression rate, the potential of the P. pastoris expression system has still not been fully explored. Cultivation of P. pastoris under optimized conditions greatly relies on the strain and is associated with certain problems such as promoter strength, sensitivity to methanol, and oxygen demand. To address these issues, different genetic engineering strategies have been employed. Advancements in promoter engineering, optimization of gene dosage and codon usage, recombinant plasmid engineering using CRISPR/Cas9 system, and directed evolution strategies have proven beneficial to the yield of cellulase expression levels. This study will systemically review recent progress in various genetic engineering strategies to enhance cellulase and xylanase expression in the P. pastoris expression system. The utilization of alcohol oxidase 1 promoter (pAOX1), methanol‐free system, and recombinant plasmid engineering for improved production of these enzymes are highlighted. Additionally, we discuss the recent advancements in the P. pastoris expression system toolbox for improved cellulase and xylanase production, thus providing a deep insight into how P. pastoris is becoming the indispensable platform for heterologous protein production. © 2023 Society of Chemical Industry (SCI).
Changes in child and adolescent mental health across the COVID‐19 pandemic (2018–2023): Insights from general population and clinical samples in the Netherlands
Authors: van Oers Hedy A. , Alrouh Hekmat , Tieskens Jacintha M. , Luijten Michiel A. J. , de Groot Rowdy , Broek Emma , van der Doelen Daniël , Klip Helen , De Meyer Ronald , van der Mheen Malindi , Ruisch I. Hyun , van den Berg Germie , Bruining Hilgo , Buitelaar Jan , van der Rijken Rachel , Hoekstra Pieter J. , Kleinjan Marloes , Lindauer Ramón , Oostrom Kim J. , Staal Wouter , Vermeiren Robert , Cornet Ronald , Haverman Lotte , Popma Arne , Bartels Meike , Polderman Tinca J. C. , Zijlmans Josjan 
Published: December 2023

AbstractBackgroundThe COVID‐19 pandemic negatively affected child and adolescent mental health and at the end of the pandemic (April 2022) child mental health had not returned to pre‐pandemic levels. We investigated whether this observed increase in mental health problems has continued, halted, or reversed after the end of the pandemic in children from the general population and in children in psychiatric care.MethodsWe collected parent‐reported and child‐reported data at two additional post‐pandemic time points (November/December 2022 and March/April 2023) in children (8–18 years) from two general population samples (N = 818–1056 per measurement) and one clinical sample receiving psychiatric care (N = 320–370) and compared these with data from before the pandemic. We collected parent‐reported data on internalizing and externalizing problems with the Brief Problem Monitor and self‐reported data on Anxiety, Depressive symptoms, Sleep‐related impairments, Anger, Global health, and Peer relations with the Patient‐Reported Outcomes Measurement Information System (PROMIS®).ResultsIn the general population, parents reported no changes in externalizing problems but did report higher internalizing problems post‐pandemic than pre‐pandemic (p < 0.001). Children also reported increased mental health problems post‐pandemic, especially in anxiety and depression, to a lesser extent in sleep‐related impairment and global health, and least in anger (all ps < 0.01). In the clinical sample, parents reported higher internalizing (p < 0.001), but not externalizing problems post‐pandemic compared to the start of the pandemic. Children reported greatest increases in problems in anxiety, depression, and global health, to a lesser extent on sleep‐related impairment, and least on anger (all ps < 0.05).ConclusionsChild mental health problems in the general population are substantially higher post‐pandemic compared to pre‐pandemic measurements. In children in psychiatric care mental health problems have increased during the pandemic and are substantially higher post‐pandemic than at the start of the pandemic. Longitudinal and comparative studies are needed to assess what the most important drivers of these changes are.
Morphological description of spontaneous pelvic fin loss in a neotropical cichlid fish
Authors: Ehemann Nicolás Roberto , Meyer Axel , Hulsey Christopher Darrin 
Published: December 2023

AbstractPelvic fins are a characteristic structure of the vertebrate Bauplan. Yet, pelvic fin loss has occurred repeatedly across a wide diversity of other lineages of tetrapods and at least 48 times in teleost fishes. This pelvic finless condition is often associated with other morphological features such as body elongation, loss of additional structures, and bilateral asymmetry. However, despite the remarkable diversity in the several thousand cichlid fish species, none of them are characterized by the complete absence of pelvic fins. Here, we examined the musculoskeletal structure and associated bilateral asymmetry in Midas cichlids (Amphilophus cf. citrinellus) that lost their pelvic fins spontaneously in the laboratory. Due to this apparent mutational loss of the pelvic girdle and fins, the external and internal anatomy are described in a series of “normal” Midas individuals and their pelvic finless sibling tankmates. First, other traits associated with teleost pelvic fin loss, the genetic basis of pelvic fin loss, and the potential for pleiotropic effects of these genes on other traits in teleosts were all reviewed. Using these traits as a guide, we investigated whether other morphological differences were associated with the pelvic girdle/fin loss. The mean values of the masses of muscle of the pectoral fin, fin ray numbers in the unpaired fins, and oral jaw tooth numbers did not differ between the two pelvic fin morphotypes. However, significant differences in meristic values of the paired traits assessed were observed for the same side of the body between morphotypes. Notably, bilateral asymmetry was found exclusively for the posterior lateral line scales. Finally, we found limited evidence of pleiotropic effects, such as lateral line scale numbers and fluctuating asymmetry between the Midas pelvic fin morphotypes. The fast and relatively isolated changes in the Midas cichlids suggest minor but interesting pleiotropic effects could accompany loss of cichlid pelvic fins.
Rapid screening and evaluation of natural antioxidants from leaf, stem, and root of Artemisia argyi by online liquid microextraction combined with HPLC‐based antioxidant assay system coupled with calibration quantitative analysis
Authors: Yang Wei‐Qi , Huang Qi , Wu Meng‐Qi , Mei Quan‐Xi , Zou Yuan‐Sheng , Qian Zheng‐Ming , Tang Dan 
Published: December 2023

To reveal the utilization value of leaf, stem, and root of Artemisia argyi, a rapid online liquid microextraction combined with a high‐performance liquid chromatography coupled with 2,2‐nitrogen‐di (3‐ethyl‐benzothiazole‐6‐sulfonic acid) diammonium salt antioxidant assay system was established for analysis of antioxidants in the leaf, stem, and root of A. argyi, and a calibration quantitative method of antioxidant activity with equivalent chlorogenic acid was proposed. Thirty‐three positive peaks were identified; among them, 12 compounds were found that possess good antioxidant activity including eleven organic acids (components 2–4, 8, 11–14, 17, 19, and 21) and one flavonoids (component 22). The proposed calibration quantitative method avoided the influence of content of compound and compared the extent of radical scavenging capacity of five antioxidant compounds, which were ranked as follow: 3,5‐dicaffeoylquinic acid > 3,4‐dicaffeoylquinic acid ≈ 4,5‐dicaffeoylquinic acid > 1,4‐dicaffeoylquinic acid > chlorogenic acid. In conclusion, this study provided composition and biological potential for the future development of the leaf, stem, and root of A. argyi. It is believed that the online liquid microextraction combined with high‐performance liquid chromatography based antioxidant assay system can be widely used for the rapid screening of natural antioxidant components in the different parts of natural products.
Anti‐Counterfeiting Application of Persistent Luminescence Materials and Its Research Progress
Authors: Zhang Jiawei , Wang Zhijun , Huo Xiaoxue , Meng Xue , Wang Yu , Suo Hao , Li Panlai 
Published: December 2023

AbstractPersistent luminescent materials can continue to emit light after stopping excitation, and their stored energy is gradually released during the decay time after persistent emitting, which makes the color and luminance changes in slow decay and plays an important role in protecting information and combating forgery. When combined with other anti‐counterfeiting technologies to form a multi‐modal and multi‐process anti‐counterfeiting design, it perfectly hides the real information and has a broad application prospect in anti‐counterfeiting logo design. A brief overview of the design of three different persistent luminescence‐based anti‐counterfeiting applications in unimodality to achieve information encryption in the time dimension. The energy stored in the trap is studied to be released by different physical stimuli and applied to stimulus‐like responsive persistent luminescent materials, and the design of persistent luminescence anti‐counterfeiting applications based on many different processes in dual‐modality, obtaining the dynamic anti‐counterfeiting effect of multiple processes in multiple modes. This work reviews the research background of persistent luminescent materials in the field of anti‐counterfeiting in recent years, compares the important breakthrough progress of persistent luminescent materials applied to anti‐counterfeiting research, and finally puts forward the current problems, future challenges, and development directions.
Antibiotic‐Loaded Ultrahigh Molecular Weight Polyethylenes
Authors: Lekkala Sashank , Inverardi Nicoletta , Yuh Jean , Wannomae Keith K. , Tierney Peyton , Sekar Amita , Muratoglu Orhun K. , Oral Ebru 
Published: December 2023

AbstractThe occurrence of periprosthetic joint infections (PJI) after total joint replacement constitutes a great burden for the patients and the healthcare system. Antibiotic‐loaded polymethylmethacrylate (PMMA) bone cement is often used in temporary spacers during antibiotic treatment. PMMA is not a load‐bearing solution and needs to be replaced by a functional implant. Elution from the ultrahigh molecular weight polyethylene (UHMWPE) bearing surface for drug delivery can combine functionality with the release of clinically relevant doses of antibiotics. In this study, the feasibility of incorporating a range of antibiotics into UHMWPE is investigated. Drug stability is assessed by thermo‐gravimetric analysis and nuclear magnetic resonance spectroscopy. Drug‐loaded UHMWPEs are prepared by compression molding, using eight antibiotics at different loading. The predicted intra‐articular concentrations of drugs eluted from UHMWPE are above minimum inhibitory concentration for at least 3 weeks against Staphylococci, which are the major causative bacteria for PJI. The antibacterial efficacy is confirmed for samples covering 2% of a representative knee implant in vitro over 72 h, showing that a small fraction of the implant surface loaded with antibiotics may be sufficient against Staphylococci.
Translating mesenchymal stem cell and their exosome research into GMP compliant advanced therapy products: Promises, problems and prospects
Authors: Ma Chui‐Yan , Zhai Yuqing , Li Chung Tony , Liu Jie , Xu Xiang , Chen Hao , Tse Hung‐Fat , Lian Qizhou 
Published: December 2023

AbstractMesenchymal stem cells (MSCs) are one of the few stem cell types used in clinical practice as therapeutic agents for immunomodulation and ischemic tissue repair, due to their unique paracrine capacity, multiple differentiation potential, active components in exosomes, and effective mitochondria donation. At present, MSCs derived from tissues such as bone marrow and umbilical cord are widely applied in preclinical and clinical studies. Nevertheless, there remain challenges to the maintenance of consistently good quality MSCs derived from different donors or tissues, directly impacting their application as advanced therapy products. In this review, we discuss the promises, problems, and prospects associated with translation of MSC research into a pharmaceutical product. We review the hurdles encountered in translation of MSCs and MSC–exosomes from the research bench to an advanced therapy product compliant with good manufacturing practice (GMP). These difficulties include how to set up GMP‐compliant protocols, what factors affect raw material selection, cell expansion to product formulation, establishment of quality control (QC) parameters, and quality assurance to comply with GMP standards. To avoid human error and reduce the risk of contamination, an automatic, closed system that allows real‐time monitoring of QC should be considered. We also highlight potential advantages of pluripotent stem cells as an alternative source for MSC and exosomes generation and manufacture.
Quality of life in children with Down syndrome and its association with parent and child demographic characteristics: Parent‐reported measures
Authors: Alrayes Nuha , Issa Noha M. , Alghubayshi Omar Y. , Al‐Amaa Jumana Y. , Alsabban Ashwaq Hassan , Al Shaer Dalal Sameer , Alyoubi Reem Abdullah , Nasser Khalidah K. , Alkhiary Yaser M. 
Published: December 2023

AbstractBackgroundThis study aims to explore the association between the quality of life (QoL) in children with Down syndrome (DS) and its relationship with demographic characteristics of both parents and children. The investigation encompasses five domains: physical and psychological well‐being, autonomy and parental relationship, social well‐being, and peers, as well as school and the learning environment.MethodAn online questionnaire, the KIDSCREEN‐27, was used to measure the QoL of 112 families with DS in Saudi Arabia, referred to as “Parent‐Reported Measures.” Descriptive statistics were analyzed using the Statistical Package for Social Sciences.ResultsThe study found that the QoL of children with DS showed high scores in the psychological well‐being, autonomy, parental relations, school, and learning environment domains. However, the physical and social well‐being and peer domains had lower scores, although still considered “good scores.” Family income had a positively significant influence on all QoL domains. Specifically, higher family income was associated with better QoL outcomes, except for social well‐being. Parental age was found to influence psychological well‐being, while parental education and the relationship between the parent and child influenced social well‐being. Lastly, the child's gender was found to have an impact on the school and learning environment domain.ConclusionThe study highlights the importance of understanding the impact of the demographic variability of children with DS and their parents on the QoL of their children. It emphasizes the need to address the needs of families with lower incomes and the importance of parental education and relationships with their children in improving social well‐being. The findings could aid policymakers and healthcare providers in improving the QoL for families with children who have DS.
Research progress and perspectives in field‐induced imprinted polymers
Authors: Xu Wan , Ou Xiaojian , Sun Yuan , Sun Yuanjun , Chen Zhenbin , Yu Xiaoqing 
Published: December 2023

AbstractIn the process of preparation of imprinted polymers, the introduction of external fields, such as magnetic field, microwave field, ultrasonic field, electric field and high‐energy radiation field, to improve the structure and performance of imprinted polymers has become a novel direction of the development of imprinted polymers, and relevant reports have been increasing in recent years. However, the progress of research on field‐induced imprinted polymers has not been summarized and noticed, resulting in few perspective on the shortcomings and future development of the field‐induced effect which in turn has hindered its further study. Considering this, we firstly introduced the development history and mechanism of imprinted polymers. Then, the field‐induced effects on the mechanism of polymerization and the structure and performances of imprinted polymers were summarized in the order of magnetic field‐induced imprinted polymers, microwave field‐induced imprinted polymers, ultrasonic field‐induced imprinted polymers, electric field‐induced imprinted polymers and high‐energy radiation field‐induced polymers. Significantly, the challenges to the development of field‐induced imprinted polymers were presented, and the development trends of field‐induced imprinted polymers were proposed with the aim to build the bridge for future FI‐IPs research and shed the light on the application research of the field‐induced imprinted polymers.
An environmentally friendly strategy for preparing polydimethylsiloxane/multiwalled carbon nanotube composites with multilayer structures
Authors: Zhang Menghui , Shao Liang , Zhang Tao , Yang Yanlong , Li Xiaoqiang , Ma Jianzhong 
Published: December 2023

AbstractThis work showcases the production of flexible polydimethylsiloxane/multiwalled carbon nanotube (PDMS/MWCNT) composites with multilayer structures via the glucose template method followed by vacuum‐assisted filtration and hot pressing. The properties of the single‐layer and multilayer composites have been systematically investigated. In the X‐band, thanks to the ohmic loss, the interfacial polarization loss at the interfaces and the multiple reflections between PDMS/MWCNT layers, the 5‐layer PDMS/MWCNT composites achieves 65.9 dB. Furthermore, all multilayer PDMS/MWCNT composites present a high retention rate of about of 90% after repeated bending with curvature radius of 1.8 mm at a frequency of 1 cycle per 2 s for 1000 times.Highlights
The PDMS/MWCNT composites are fabricated by the glucose template method.
The 5‐layer PDMS/MWCNT composites can reach 65.9 dB in X‐band.
The PDMS/MWCNT composites present high stability even after repeated bending.
Functional surfaces by plasma grafting of itaconic acid onto polypropylene mesh: synthesis and structural investigations
Authors: Sethi Vipula , Verma Chetna , Mukhopadhyay Samrat , Gupta Amlan , Gupta Bhuvanesh 
Published: December 2023

AbstractPolypropylene (PP) is an unflinching element of biomaterials for human healthcare, leading to enormous possibilities for their functional development within a broad range of biocompatible and bioreceptive materials. Plasma grafting of itaconic acid was carried out on a PP surface by oxygen plasma activation. The variation of grafting parameters, such as monomer concentration, reaction temperature and reaction time, as a function of the degree of grafting was investigated. The graft distribution and surface homogeneity of the PP surfaces were evaluated using confocal microscopy, field emission scanning electron microscopy and atomic force microscopy. These surface characterizations led to information about the impact of grafts on the material surface. © 2023 Society of Industrial Chemistry.