bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Investigation of Recombinant Human TMPRSS4 Expression and Purification Across Diverse Expression Platforms: Bacterial, Insect (BVES), and Mammalian Systems
Authors: Esram Poornima , Arumugam Premkumar 
Published: October 2023

TMPRSS4, an essential transmembrane protease serine 4, holds significant relevance in diverse biological contexts, making it a molecule of interest across various fields. This transmembrane serine protease, TMPRSS4, plays a pivotal role in multiple areas, including cancer research, disease processes, and potentially in the spread of viral infections. In light of its multifaceted significance, the research and production of active TMPRSS4 protein serve as a critical foundation for in-depth structural and functional studies, ultimately leading to the identification of potential inhibitors. Within this context, our study encompasses an extensive investigation of various expression systems, including microbial, insect cells, and mammalian cells, aimed at obtaining biologically active TMPRSS4 protein. This study underscores our efforts in optimizing solubility. We explored different E. coli expression strains, optimized expression conditions at various temperatures, employed both inducible and autoinduction-based approaches, fine-tuned the Multiplicity of Infection (MOI), and controlled cell density in Sf9 cells using BVES. Additionally, we delved into the exploration of alternative signal peptides for secretory expression in mammalian cells, followed by the characterization of purified protein variants. Through rigorous biochemical analyses and functional assays, we discovered the distinct advantages associated with mammalian cell expression, ultimately resulting in the production of biologically active TMPRSS4. Our findings provide critical insights into the optimization of expression systems, thereby enhancing the functionality of the protein.
Instrumentos de evaluación de la funcionalidad familiar: una revisión integrativa
Authors: ROA AYA KEVIN , RODRIGUEZ LOPEZ JAVIER ISIDRO , APONTE FRANCO SANDRA MILENA 
Published: October 2023

Introducción
Para la valoración familiar en cuanto a su contexto, su dinámica y funcionamiento en los últimos años se han generado diversos instrumentos de evaluación, actualmente en la literatura científica no se encuentra una revisión que permitiera compilar y visualizar los instrumentos que midan la funcionalidad familiar y se encuentren validados para la región de América Latina.
Objetivo
Identificar los instrumentos que evalúen la funcionalidad familiar y se encuentren validados en Latinoamérica en idioma español.
Metodología
Se realizó una revisión integrativa donde se utilizaron los siguientes descriptores salud familiar, psicometría, enfermería familiar, enfermería usando en la estrategia de búsqueda los operadores booleanos OR y AND en los idiomas inglés y español, se utilizaron las bases de datos: Biblioteca Virtual en Salud (BVS), Cochrane, Science Direct, Redalyc, Google académico.
Resultados
Se retienen finalmente doce artículos los cuales fueron desarrollados en Colombia, Perú, Uruguay, México, Chile y Argentina uno en cada país; estos estudios fueron publicados entre el año 2002 hasta el 2017. Los estudios realizaron pruebas como el KMO, la prueba de Bartlett y el Alfa de Cronbach. Los instrumentos reportados y validados a los contextos locales son el APGAR Familiar, FACES III y IV y ASF-E.
Conclusión
Se necesita mayor desarrollo de instrumentos confiables y validados para América Latina y su aplicación en los contextos locales, lo que permita generar información con mayor exactitud para tomar acciones de intervención familiar efectivas y toma de decisiones adecuadas.
Assessment of maxillary anterior teeth using RED proportion and golden proportion among adult young Gujarati individuals
Authors: Dave Bhagyashree H , Mehta Shruti P , Sutaria Shreyansh P , Shah Setu P 
Published: October 2023

Comprehensive studies assessing the spatial examination of maxillary anterior teeth with respect to various esthetic proportions like RED proportion and Golden proportion are insufficient for Gujarati Individual.: The purpose of this study is to assess the validity of Recurring Esthetic Dental (RED)Proportion and Golden proportion in maxillary anterior teeth among young adult Gujarati individual.: This clinical Evaluation study included a total of 70 Gujarati individuals that met the inclusion criteria having 40 females and 30 males. Dental casts of the maxillary arches for each individual were made with irreversible hydrocolloid material and dental stone was then poured to get the diagnostic casts. Using a digital caliper, dimensions and the perceived width of the anterior teeth from the front were assessed. The data was analyzed using SPSS. The RED proportion and Golden proportion were not in co-relation between perceived maxillary anterior teeth widths of Gujarati individuals. The Golden proportion and the RED proportion cannot be used as constant proportions to create a harmonious smile.
Full mouth prosthetic rehabilitation using hobo’s philosophy: A case report
Authors: Patil Rahul 
Published: October 2023

The face, which is the part of the body that is most exposed, has the mouth as a prominent feature. The allure of the teeth draws all eyes. Some patients with collapsed bites were required to have all of their teeth extracted and replaced with traditional complete dentures because rehabilitation techniques' efficacy was questioned in the 20th century. The occlusal surfaces of a patient's teeth will gradually deteriorate over the course of their lifetime. But excessive occlusal wear can result in pulpal injury, occlusal discord, functional impairment, and aesthetic deformity. Thanks to improvements in dental technology, equipment, and materials over the last millennium, the task of restoring and rehabilitating the damaged dentition has gotten simpler. This has given restorative dentists the ability to maintain teeth and associated structures. Establishing optimal oral health should be the main objective of all rehabilitation procedures because maintaining oral health and safeguarding the stomatognathic system will always be the end goal. The report's case study serves as an illustration of Hobos' full mouth rehabilitation technique.
Cardiac implantable electronic device infection: Microbiology and antibiotic prophylaxis
Authors: Paralkar Shraddha Shivling , Godbole Deepa , Mundhe Swapnil 
Published: October 2023

Cardiovascular implantable electronic devices (CIED) improve quality of life of patients with cardiac arrhythmias and also improves chances of survival. CIEDs, however it may cause complications. To avoid these complications surgical prophylaxis in CIED insertion is required to avoid infection. Due to the rise in antimicrobial resistance the use of antimicrobial agents should be rational and under control. To prevent resistance of antibiotics their use and duration of therapy should be monitored. The high-end and restricted antibiotics should be used only if organisms grow in cultures or if suggested by infectious disease specialists. This review focuses on empirical antibiotics used as prophylaxis. The purpose of this document is to outline the antimicrobial options which can be used as an empirical prophylactic agent in CIED infections.
Wood rotting polyporales from the biodiversity reserves within the Indian subtropical habitat
Authors: Chawngthu Zohmangaiha , Tluanga Lalnun , Zothanzama John , Thachunglura V L , Lalbiakmawia Benjamin , Renthlei Laltanpuia 
Published: October 2023

Identification of fungi to species level is paramount in both basic and applied applications in scientific research. A diverse group of fungi play a crucial role in ecosystem functioning and significantly contribute to the biodiversity of various ecosystems. In the northeastern state of Mizoram, India, the study of wood-inhabiting fungi holds immense potential for uncovering the region's fungal diversity and ecological interactions. The present study investigated the phylogenetic relationships and taxonomic identities of different fungal species of polypores isolated from diverse parts of Mizoram, India. Fungal species were collected and identified using a macro and micro-morphological characteristics and molecular approaches. Nuclear ribosomal DNA sequences, along with the internal transcribed spacers (ITS-1 and ITS-2), and the 5.8S gene area, were used to identified the collected samples. 23 fungal isolates of polypores were selected for molecular phylogenetic analysis. The 23 species were identified to the species level based on the fungal sequences with known identities in GenBank.The combined approach both morphological and molecular techniques proposed in the study holds the potential to address these challenges and provide a more efficient and accurate way of understanding fungal distribution.
A rare first case of prosthetic knee joint infection by Citrobacter koseri
Authors: Kagzi Yusuf , Bohra Mustafa Siraj , Kagzi Alifiya , Rizwan Umer 
Published: October 2023

Citrobacter generally colonizes the gastrointestinal and urogenital tracts of humans, but musculoskeletal infection by Citrobacter in the elderly is extremely rare. To our knowledge, less than 15 cases of causing musculoskeletal infection have been reported in the literature. In the last few years, there has been a considerable increase in the incidence of prosthetic knee joint infection in the United States, with a higher risk of infection in the first two years following surgery. Although gram-positive bacteria like Staphylococcus and Streptococci are more common cause of PJI, gram-negative bacteria can also be found in infected joints. We report the first case of PJI in the left knee due to in an 82-year-old adult two years after bilateral knee arthroplasty. This case report intends to educate and raise awareness for causing PJI as a long term post-op complication even in healthy individuals. We searched articles on Pubmed and Medline for PJI and citrobacter infection. We report clinically and microbiologically confirmed in a synovial fluid sample of the left knee joint. should be considered as a possible pathogen for PJI in long term setting even in an otherwise healthy individual.
The Benefits of Telehealth on Quality of Life of People Living with Type 2 Diabetes Mellitus: A Literature Review
Authors: Jannah Fitriatul , Safutra Suraya Andi , Erindia Firnanda , Nurwahidah Nurwahidah , Chayatush Sholichah Ahla 
Published: October 2023

Introduction: Advancement technology and information benefit societies by way telehealth access, including those with diabetes mellitus. Telehealth implementation in diabetes mellitus management is a great solution of increasing access to healthcare and reducing geographical inequalities. Nevertheless, the effectiveness of service among people living with diabetes remains inconsistant. This review aims to summarize and evaluate the evidence on the effectiveness of telehealth on quality of life in type 2 diabetes mellitus
Method: This review considers the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) guideline. The results reported in descriptive narrative approach. The following databases; Scopus, Science Direct, PubMed, and Web of Science were comprehensively searched to find article from January, 1st 2018 to December 31st 2023. Inclusion was made to English and full-text article.
Results: Two articles were included in the final review. A total of 482 type 2 DM patients were used telehealth. Findings on the impacts of the usability of telehealth on the QoL of Type 2 DM is telehealth relevant alternative to the care of DFU patients, with stable health, well-being and quality of life conditions and smartphone application-based interventions in the management of type 2 DM show that the quality of life in the following four dominants; psychological, social, environmental, and physical health revealed significant improvements
Conclusions: Telehealth has good potential to be developed in the service of diabetes mellitus patients. The following internet-based delivered mode; web, and smartphones improve the quality of life and the well-being of patients with type 2 diabetes mellitus. However, lack of studies measuring the effectivity of telehealth among people with diabetes mellitus. Future development of internet-based interventions should further take into consideration the consolidated set of diabetes mellitus principles.
Keyword: Type 2 Diabetes Mellitus, Quality of Life, Telehealth, Nursing Practice, Nursing Care
Impact of Multimodal Intervention on Quality of Life of Geriatric Clients regarding Health Promotional Outcomes at a selected Hospital, Kolar, Karnataka, India
Authors: VANI VANI .R , Cariena Zeanath Cariena Joseph Zeanath , Reshma Priya Reshma Aranha Priya 
Published: October 2023

Abstract
Purpose: To understand the Quality of Life of geriatric clients, that influence through Multimodal Intervention.
Methods: Quantitative approach Quasi-Experimental interventional controlled study with pre-test post-test design and follow-up for two months, adopted by using purposive sampling technique among 120 geriatric clients who gave consent for participation in the study at two different settings of hospitals from 28/07/2022 to 10/01/2023, Kolar. After obtaining CEC, data was obtained using the WHOQOL questionnaire and the Multimodal Intervention package consisting of a Snake & Ladder Health promotion strategies game, Educational Video, and Informational pamphlet been distributed to the experimental group whereas routine care given to the Control group followed by Posttest on the 30th day & 60th day. Reinforcement was carried out by the investigator on fortnightly basis reminders through messages. Data were analyzed by using Descriptive & Inferential statistics such as RMANOVA, Independent‘t’-test, Paired‘t-test, and Chi-square.
Results: A significant effect was demonstrated in the experimental group with enhanced mean, SD from pretest to posttest I and Posteest II found to be 54.76±10.39, 74.15±6.75& 77.71±4.99 without any significant changes in the control group.
Conclusion: Geriatric population was the biggest beneficiary, Multimodal Intervention was proven to be effective and can be implemented in hospitals, and community settings in improving the Quality of life of geriatric clients to foster healthy aging.
Scope and Challenges in the Implementation of Time Bank in India: A Qualitative Study
Authors: Verma Ankita , Srid Sruthi , Fatima Kaneez , Asthana Manish Kumar 
Published: October 2023

Abstract

This paper explores the scope of services and challenges in implementing Time Bank in India. This community-based initiative allows people to exchange skills and services without the involvement of money. A qualitative study was conducted using semi-structured telephone interviews with 20 participants aged 18 to 35. Thematic analysis revealed four major themes: Services, Challenges and Limitations of Time Bank, Factors Affecting Help-Seeking Behavior, and Community and Gender Dynamics. Participants expressed interest in offering a wide range of services, from household tasks to professional services. However, challenges and limitations were also identified, such as a lack of awareness and understanding of the concept, social stigma, and concerns about trust, privacy, and safety issues. The study underscores the need to consider cultural and social factors while implementing community-based initiatives. Despite these challenges, participants believed Time Bank could be a valuable platform for building social connections and community support. The study's implications highlight the need for further research on the impact of Time Bank on social capital and the sustainability of the initiative in different cultural contexts.
C-SuFEx Linkage of Sulfonimidoyl Fluorides and Organotrifluoroborates
Authors: Jiang Xuefeng , Zhao Suqin , Zeng Daming , Wang Ming 
Published: October 2023

Abstract
Sulfur(VI) fluoride exchange (SuFEx), a new type of linkage reaction, has excellent potential for application in functional molecule linkage to prepare pharmaceuticals, biomolecules, and polymers. Herein, a C-SuFEx catalytic reaction was established to achieve fast (in minutes) linkage between sulfonimidoyl fluorides and aryl/alkyl organotrifluoroborates. Potassium organotrifluoroborates were instantaneously activated via trimethylsilyl triflate to afford organodifluoroboranes, releasing BF3 as a catalyst in situ. This C-SuFEx technique was capable of forming S(VI)-C(alkyl), S(VI)-C(alkenyl) and S(VI)-C(aryl) bonds, demonstrating its broad scope and thus surpassing the achievements of previous studies. Natural products and pharmaceuticals with sensitive functional groups, such as valdecoxib, celecoxib and diacetonefructose, are compatible with this protocol, allowing the formation of diverse sulfoximines.
FKBP5 drives bone marrow stem cells senescence and suppresses osteogenic differentiation via canonical WNT/β-catenin signaling pathway
Authors: Zhu Bin , Cai Bowen , Xue Kaixiao , Yin Guoyong , Zhou Shumin , Fang Jiahu 
Published: October 2023

Abstract
Senile osteoporosis and associated fractures significantly increase the morbidity and mortality of older people, thus increasing the cost of public health. Further investigations are required to explore the molecular causes of senile osteoporosis. In this study, FKBP5 expression in bone marrow mesenchymal stem cells (BMSCs) increased with age, and the degree of expression was inversely related to the patient's bone mineral density or CT values. Functional studies have validated the regulatory function of FKBP5 in BMSCs osteogenesis differentiation through the canonical WNT/β-catenin signaling pathway by binding to β-catenin and promoting its ubiquitination and degradation. Administration of SAFit2, a selective inhibitor of FKBP5, enhanced bone density in an animal model of senile osteoporosis. These findings suggest that FKBP5 may be a novel target and offer a new perspective on osteoporosis treatment.
Exploration of systematic treatment strategies for anaplastic thyroid carcinoma: A single-institutional retrospective study
Authors: Xu Jiaojiao , Chen Keyu , Fang Ziru , Ye Hunan , Wang Xiaojia , Fang Meiyu , Cao Jun 
Published: October 2023

Abstract
Purpose: Anaplastic thyroid carcinoma (ATC) has high invasiveness, poor clinical prognosis, and high mortality rates. The article aimed to pinpoint the treatment characteristics of ATC.
Method: A retrospective analysis was conducted on 61 patients diagnosed with ATC at our institution from March 1, 2003 to September 30, 2022. All of them were diagnosed pathologically and their information regarding clinicopathologic characteristics, treatment strategy, and follow-up events was collected. The Kaplan-Meier method was used for survival analysis. Then, the Cox proportional hazards model was used to calculate the univariate and multivariate analyses.
Result: The study included 61 patients with ATC, ranging from 34 to 85 years old, with a median age of 66 in ATC. Half of (30/61, 49.2%) these patients’ tumor size was larger than 5cm. At the first prognosis, about 43/61 (70.5%) ATC patients accompanied acute symptomatic. 37 (60.7%) patients received chemotherapy, 11 (18%) patients treated with targeted therapy, and 9 (14.8%) ATC patients received immunotherapy. Surgery combines with chemotherapy could improve overall survival in ATC (P=0.003). 9 ATC patients who received targeted therapy based on surgery were able to increase their overall survival (P=0.025). Immunotherapy based on surgery was administered to 9 patients with ATC, statistical analysis also indicated beneficial (P=0.004).
Conclusion: Patients with ATC can be benefited from a systematic regimen and immunotherapy may be a new therapeutic direction. The multimodality pattern of ATC with the aid of biomarkers warrants exploration.
Optimized Finite-Time Integral Fast Terminal Sliding Mode Control for Leukemia Cancer Treatment
Authors: Hazareh Gozar Ali , Ghadiri Hamid , Ranjbar Mohammad Ali , Nazari Ali Jamali , Bacanin Nebojsa , Rezvani Mohammad Hossein 
Published: October 2023

Abstract
Leukemia is a type of blood cancer that affects the bone marrow and lymphatic system. Chemotherapy as a drug treatment method is one of the popular ways of treating this disease to destroy fast-growing cells. In this paper, the finite-time adaptive integral fast terminal sliding mode control (AIFTSMC) as a robust strategic method for treating leukemia cancer based on the chemotherapy process has been introduced. Two different treatment modes called uniform and non-uniform have been investigated in detail. Our goal in this trial is to reduce the number of cancer cells during treatment while minimizing damage to healthy cells. Moreover, the controller's coefficients in the sliding surface have been optimized using the water cycle algorithm, a novel type of metaheuristic algorithm. The simulation results show that AIFTSMC effectively targets cancer cells while minimizing damage to healthy cells. The results promise a novel and practical way to treat leukemia in clinical applications.
MHD boundary layer flow of ternary hybrid nano-fluid (𝐓𝐢𝐨𝟐 - 𝐒𝐢𝐨𝟐, - 𝐀𝐥𝟐𝐨𝟑⁄𝐇𝟐𝐎 - 𝐂𝟐𝐇𝟔𝐎𝟐) A Numerical and analytical Investigations
Authors: KEZZAR Mohamed , NAFIR Noureddine , DIB Amar , SARI Mohamed Rafik , Dwivedi Naveen , Noor N. F. M. , TABET Ismail 
Published: October 2023

Abstract
In the present work, we explored the magneto hydrodynamic (MHD) flow in the pressure gradient across a flat plate. The effects of the magnetic field and the addition of ternary hybrid nano-fluid (i.e. a mixture of three nano particles for example ( \({Tio}_{2}, {Sio}_{2, } and {Al}_{2}{o}_{3}\)) in mixture base fluid ( \({H}_{2}O-{C}_{2}{H}_{6}{O}_{2}\) ) are also considered in this study. Basic partial differential equations (PDEs) are transformed into nonlinear ordinary differential equations using similarity transformation. Then, this equation was treated numerically by the 4th–5th order of the Runge-Kutta-Fehlberg method with shooting approaches and analytically by a new method called the Daftardar-Jafari Method (DJM). The effect of various physical parameters namely the magnetic field (M), the volumetric fraction of the nanoparticles, the pressure gradient \(\left(m\right)\) on the velocity distribution, the type of the nanoparticles and the coefficient of friction \({\text{C}}_{\text{f}}\), of the theoretical results obtained confirm that the ternary hybrid nano-fluid has a better dynamic property than the hybrid nano-fluid, as well as the efficiency of the adopted DJM technique.