bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer
Authors: Pecci Valeria , Troisi Fabiola , Aiello Aurora , De Martino Sara , Carlino Angela , Fiorentino Vincenzo , Ripoli Cristian , Rotili Dante , Pierconti Francesco , Martini Maurizio , Porru Manuela , Pinto Francesco , Mai Antonello , Bassi Pier Francesco , Grassi Claudio , Gaetano Carlo , Pontecorvi Alfredo , Strigari Lidia , Farsetti Antonella , Nanni Simona 
Published: February 2024

Abstract
Background
About 30% of Prostate cancer (PCa) patients progress to metastatic PCa that remains largely incurable. This evidence underlines the need for the development of innovative therapies. In this direction, the potential research focus might be on long non-coding RNAs (lncRNAs) like H19, which serve critical biological functions and show significant dysregulation in cancer. Previously, we showed a transcriptional down-regulation of H19 under combined pro-tumoral estrogen and hypoxia treatment in PCa cells that, in turn, induced both E-cadherin and β4 integrin expression. H19, indeed, acts as transcriptional repressor of cell adhesion molecules affecting the PCa metastatic properties. Here, we investigated the role of H19/cell adhesion molecules circuitry on in vivo PCa experimental tumor growth and metastatic dissemination models.

Methods
H19 was silenced in luciferase-positive PC-3 and 22Rv1 cells and in vitro effect was evaluated by gene expression, proliferation and invasion assays before and after treatment with the histone lysine demethylase inhibitor, GSK-J4. In vivo tumor growth and metastasis dissemination, in the presence or absence of GSK-J4, were analyzed in two models of human tumor in immunodeficient mice by in vivo bioluminescent imaging and immunohistochemistry (IHC) on explanted tissues. Organotypic Slice Cultures (OSCs) from fresh PCa-explant were used as ex vivo model to test GSK-J4 effects.

Results
H19 silencing in both PC-3 and 22Rv1 cells increased: i) E-cadherin and β4 integrin expression as well as proliferation and invasion, ii) in vivo tumor growth, and iii) metastasis formation at bone, lung, and liver. Of note, treatment with GSK-J4 reduced lesions. In parallel, GSK-J4 efficiently induced cell death in PCa-derived OSCs.

Conclusions
Our findings underscore the potential of the H19/cell adhesion molecules circuitry as a targeted approach in PCa treatment. Modulating this interaction has proven effective in inhibiting tumor growth and metastasis, presenting a logical foundation for targeted therapy.
The interplay between obesity, immunosenescence, and insulin resistance
Authors: Shimi Ghazaleh , Sohouli Mohammad Hassan , Ghorbani Arman , Shakery Azam , Zand Hamid 
Published: February 2024

AbstractObesity, which is the accumulation of fat in adipose tissue, has adverse impacts on human health. Obesity-related metabolic dysregulation has similarities to the metabolic alterations observed in aging. It has been shown that the adipocytes of obese individuals undergo cellular aging, known as senescence. Senescence can be transmitted to other normal cells through a series of chemical factors referred to as the senescence-associated secretory phenotype (SASP). Most of these factors are pro-inflammatory compounds. The immune system removes these senescent T-cells, but immunosenescence, which is the senescence of immune cells, disrupts the clearance of senescent T-cells. Immunosenescence occurs as a result of aging or indirectly through transmission from senescent tissues. The significant occurrence of senescence in obesity is expected to cause immunosenescence and impairs the immune response to resolve inflammation. The sustained and chronic inflammation disrupts insulin's metabolic actions in metabolic tissues. Therefore, this review focuses on the role of senescent adipocyte cells in obesity-associated immunosenescence and subsequent metabolic dysregulation. Moreover, the article suggests novel therapeutic approaches to improve metabolic syndrome by targeting senescent T-cells or using senotherapeutics.
Graphical Abstract
The impact of goal-directed fluid therapy on postoperative pulmonary complications in patients undergoing thoracic surgery: a systematic review and meta-analysis
Authors: Han Shuang , Wu Xiaoqian , Li Pan , He Kun , Li Jianli 
Published: February 2024

Abstract
Background
Pulmonary complications after thoracic surgery are common and associated with significant morbidity and high cost of care. Goal-directed fluid therapy (GDFT) could reduce the incidence of postoperative pulmonary complications (PPCs) and facilitate recovery in patients undergoing major abdominal surgery. However, whether GDFT could reduce the incidence of PPCs in patients undergoing thoracic surgery was unclear. The present meta-analysis was designed to assess the impact of Goal-directed Fluid Therapy on PPCs in patients undergoing thoracic surgery.

Methods
Randomized controlled trials (RCTs) comparing GDFT with other conventional fluid management strategies in adult patients undergoing thoracic surgery were identified. Databases searched included PubMed, Web of Science, Embase, and Cochrane Library databases. Review Manager 5.4 (The Cochrane Collaboration, Oxford, UK) software was used for statistical analysis. Heterogeneity was analyzed using I2 statistics, and a standardized mean difference with 95% CI and P value was used to calculate the treatment effect for outcome variables. The primary study outcomes were the incidence of PPCs. Secondary outcomes were the total volume infused, the length of hospitalization, the incidence of cardiac complications, and the incidence of renal dysfunction. Subgroup analysis was planned to verify the definite role of GDFT.

Results
A total of 6 RCTs consisting of 680 patients were included in this meta-analysis, which revealed that GDFT did not reduce the incidence of PPCs in patients undergoing thoracic surgery (RR, 0.57; 95% CI 0.29–1.14). However, GDFT decreased the total intra-operative fluid input (MD, − 244.40 ml; 95% CI − 397.06 to − 91.74). There was no statistical difference in the duration of hospitalization (MD; − 1.31, 95% CI − 3.00 to 0.38), incidence of renal dysfunction (RR, 0.62; 95% CI 0.29–1.35), and incidence of cardiac complications (RR, 0.62; 95% CI 0.27–1.40).

Conclusions
The results of this meta-analysis indicate that GDFT did not reduce the postoperative incidence of pulmonary complications in individuals undergoing thoracic surgery. However, considering the small number of contributing studies, these results should be interpreted with caution.
Procedures to combine estimators of greenhouse gases emission factors
Authors: Marujo Ernesto C. , Rodrigues Gleice G. , Covatti Arthur A. 
Published: February 2024

Abstract
Background
This article describes a new procedure to estimate the mean and variance of greenhouse gases (GHG) emission factors based on different, possibly conflicting, estimates for these emission factors. The procedure uses common information such as mean and standard deviation usually reported in IPCC (Intergovernmental Panel on Climate Change) database and other references in the literature that estimate emission factors. Essentially, it is a procedure in the class of meta-analysis, based on the computation of $${S}_{a}^{2}$$

S

a

2

, a new estimator for the variance of the emission factor.

Results
We discuss the quality of this estimator in terms of its probability distribution and show that it is unbiased. The resulting confidence interval for the mean emission factor is tighter than those that would have resulted from using other estimators such as pooled variance and thus, the new procedure improves the accuracy in estimating GHG emissions.
The application of the procedure is illustrated in a case study involving the estimation of methane emissions from rice cultivation.

Conclusions
The estimation of emission factors using $${S}_{a}^{2}$$

S

a

2

was demonstrated to be more accurate because it is not biased and more precise than alternative methods.
The androgen receptor interacts with GATA3 to transcriptionally regulate a luminal epithelial cell phenotype in breast cancer
Authors: Hosseinzadeh Leila , Kikhtyak Zoya , Laven-Law Geraldine , Pederson Stephen M. , Puiu Caroline G. , D’Santos Clive S. , Lim Elgene , Carroll Jason S. , Tilley Wayne D. , Dwyer Amy R. , Hickey Theresa E. 
Published: February 2024

AbstractBackgroundThe androgen receptor (AR) is a tumor suppressor in estrogen receptor (ER) positive breast cancer, a role sustained in some ER negative breast cancers. Key factors dictating AR genomic activity in a breast context are largely unknown. Herein, we employ an unbiased chromatin immunoprecipitation-based proteomic technique to identify endogenous AR interacting co-regulatory proteins in ER positive and negative models of breast cancer to gain new insight into mechanisms of AR signaling in this disease.ResultsThe DNA-binding factor GATA3 is identified and validated as a novel AR interacting protein in breast cancer cells irrespective of ER status. AR activation by the natural ligand 5α-dihydrotestosterone (DHT) increases nuclear AR-GATA3 interactions, resulting in AR-dependent enrichment of GATA3 chromatin binding at a sub-set of genomic loci. Silencing GATA3 reduces but does not prevent AR DNA binding and transactivation of genes associated with AR/GATA3 co-occupied loci, indicating a co-regulatory role for GATA3 in AR signaling. DHT-induced AR/GATA3 binding coincides with upregulation of luminal differentiation genes, includingEHFandKDM4B, established master regulators of a breast epithelial cell lineage. These findings are validated in a patient-derived xenograft model of breast cancer. Interaction between AR and GATA3 is also associated with AR-mediated growth inhibition in ER positive and ER negative breast cancer.ConclusionsAR and GATA3 interact to transcriptionally regulate luminal epithelial cell differentiation in breast cancer regardless of ER status. This interaction facilitates the tumor suppressor function of AR and mechanistically explains why AR expression is associated with less proliferative, more differentiated breast tumors and better overall survival in breast cancer.
Broken-fat pad sign: a characteristic radiographic finding to distinguish between knee rheumatoid arthritis and osteoarthritis
Authors: Wang Qizheng , Zhao Weili , Ji Xiaoxi , Chen Yongye , Liu Ke , Zhu Yupeng , Yan Ruixin , Qin Siyuan , Xin Peijin , Lang Ning 
Published: February 2024

Abstract
Objectives
Diagnostic imaging plays an important role in the pre-treatment workup of knee osteoarthritis (OA) and rheumatoid arthritis (RA). Herein, we identified a useful MRI sign of infrapatellar fat pad (IPFP) to improve diagnosis.

Methods
Eighty-one age- and sex-matched RA and OA patients each, with pathological diagnosis and pre-treatment MRI were retrospectively evaluated. All randomized MR images were blinded and independently reviewed by two radiologists. The assessment process included initial diagnosis, sign evaluation, and final diagnosis, with a 3-week interval between each assessment. Broken-fat pad (BFP) sign was assessed on sagittal T2-weighted-imaging in routine MRI. The area under the curve and Cohen’s kappa (κ) were used to assess the classification performance. Two shape features were extracted from IPFP for quantitative interpretation.

Results
The median age of the study population was 57.6 years (range: 31.0–78.0 years). The BFP sign was detected more frequently in patients with RA (72.8%) than those with OA (21.0%). Both radiologists achieved better performance by referring to the BFP sign, with accuracies increasing from 58.0 to 75.9% and 72.8 to 79.6%, respectively. The inter-reader correlation coefficient showed an increase from fair (κ = 0.30) to substantial (κ = 0.75) upon the consideration of the BFP sign. For quantitative analysis, the IPFP of RA had significantly lower sphericity (0.54 ± 0.04 vs. 0.59 ± 0.03, p < 0.01). Despite larger surface-volume-ratio of RA (0.38 ± 0.05 vs. 0.37 ± 0.04, p = 0.25) than that of OA, there was no statistical difference.

Conclusions
The BFP sign is a potentially important diagnostic clue for differentiating RA from OA with routine MRI and reducing misdiagnosis.

Critical relevance statement
With the simple and feasible broken-fat pad sign, clinicians can help more patients with early accurate diagnosis and proper treatment, which may be a valuable addition to the diagnostic workup of knee MRI assessment.

Key points
• Detailed identification of infrapatellar fat pad alterations of patients may be currently ignored in routine evaluation.
• Broken-fat pad sign is helpful for differentiating rheumatoid arthritis and osteoarthritis.
• The quantitative shape features of the infrapatellar fat pad may provide a possible explanation of the signs.
• This sign has good inter-reader agreements and is feasible for clinical application.

Graphical Abstract
Salivary gland secretory carcinoma presenting as a cervical soft tissue mass: a case report
Authors: Mokhles Parisa , Sadeghipour Alireza , Babaheidarian Pegah , Mohebbi Saleh , Keshtpour Amlashi Zahra , Gharib Mohammad Hadi , Ahmadi Mohammad Saeid , Khastkhodaei Zeinab 
Published: February 2024

Abstract
Background
Secretory carcinoma (SC) has been described as a distinct salivary gland tumor in the fourth edition of the World Health Organization (WHO) classification of head and neck tumors. SC is generally considered as a slow-growing low-grade malignant tumor, while several cases have been reported with high-grade features, and even metastases in the literature up until now. In this article, a soft tissue SC case is discussed with high-grade microscopic features and neural invasion. A review of the salivary gland SC cases with aggressive behavior is also debated.

Case presentation
A 65-year-old Caucasian man presented with a left neck mass for the past six months. The imaging studies demonstrated a very large cystic cervical mass (46 × 23 mm) with papillary projections in the anterolateral aspect of the left neck zone Vb. He underwent left radical neck dissection (level I-V) and was followed up for 12 months with the diagnosis of Secretory carcinoma.

Conclusion
Although SC generally has a good outcome, multiple recurrences and unusual metastases may occur, which should be considered by either the pathologists or clinicians.
Effects of training flights of combat jet pilots on parameters of airway function, diffusing capacity and systemic oxidative stress, and their association with flight parameters
Authors: Bojahr Janina , Jörres Rudolf A. , Kronseder Angelika , Weber Frank , Ledderhos Carla , Roiu Immanuel , Karrasch Stefan , Nowak Dennis , Teupser Daniel , Königer Christian 
Published: February 2024

Abstract
Background
Fighter aircraft pilots are regularly exposed to physiological challenges from high acceleration (Gz) forces, as well as increased breathing pressure and oxygen supply in the support systems. We studied whether effects on the lung and systemic oxidative stress were detectable after real training flights comprising of a wide variety of exposure conditions, and their combinations.

Methods
Thirty-five pilots of the German Air Force performed 145 flights with the Eurofighter Typhoon. Prior to and after flight lung diffusing capacity for carbon monoxide (DLCO) and nitric oxide (DLNO), alveolar volume (VA), and diffusing capacities per volume (KCO, KNO) were assessed. In addition, the fractional concentration of exhaled nitric oxide (FeNO) was determined, and urine samples for the analysis of molecular species related to 8-hydroxy-2’-deoxyguanosine (8-OHdG) were taken. For statistical analysis, mixed ANOVA models were used.

Results
DLNO, DLCO, KNO, KCO and VA were reduced (p < 0.001) after flights, mean ± SD changes being 2.9 ± 5.0, 3.2 ± 5.2, 1.5 ± 3.7, 1.9 ± 3.7 and 1.4 ± 3.1%, respectively, while FeNO decreased by 11.1% and the ratio of 8-OHdG to creatinine increased by 15.7 ± 37.8%. The reductions of DLNO (DLCO) were smaller (p < 0.001) than those of KNO (KCO). In repeated flights on different days, baseline values were restored. Amongst various flight parameters comprising Gz-forces and/or being indicative of positive pressure breathing and oxygenation support, the combination of long flight duration and high altitude appeared to be linked to greater changes in DLNO and DLCO.

Conclusions
The pattern of reductions in diffusing capacities suggests effects arising from atelectasis and increased diffusion barrier, without changes in capillary blood volume. The decrease in exhaled endogenous NO suggests bronchial mucosal irritation and/or local oxidative stress, and the increase in urinary oxidized guanosine species suggests systemic oxidative stress. Although changes were small and not clinically relevant, their presence demonstrated physiological effects of real training flights in a modern 4th generation fighter jet.
Role of HOXA1-4 in the development of genetic and malignant diseases
Authors: Wang Lumin , Sun Haifeng , Cao Li , Wang Jinhai 
Published: February 2024

AbstractThe HOXA genes, belonging to the HOX family, encompass 11 members (HOXA1-11) and exert critical functions in early embryonic development, as well as various adult processes. Furthermore, dysregulation of HOXA genes is implicated in genetic diseases, heart disease, and various cancers. In this comprehensive overview, we primarily focused on the HOXA1-4 genes and their associated functions and diseases. Emphasis was placed on elucidating the impact of abnormal expression of these genes and highlighting their significance in maintaining optimal health and their involvement in the development of genetic and malignant diseases. Furthermore, we delved into their regulatory mechanisms, functional roles, and underlying biology and explored the therapeutic potential of targeting HOXA1-4 genes for the treatment of malignancies. Additionally, we explored the utility of HOXA1-4 genes as biomarkers for monitoring cancer recurrence and metastasis.
Thermal tolerance in an extremophile fish from Mexico is not affected by environmental hypoxia
Authors: Pacher Korbinian , Hernández-Román Natalia , Juarez-Lopez Alejandro , Jiménez-Jiménez Jesús Emmanuel , Lukas Juliane , Sevinchan Yunus , Krause Jens , Arias-Rodríguez Lenin , Bierbach David 
Published: February 2024

ABSTRACT
The thermal ecology of ectotherm animals has gained considerable attention in the face of human-induced climate change. Particularly in aquatic species, the experimental assessment of critical thermal limits (CTmin and CTmax) may help to predict possible effects of global warming on habitat suitability and ultimately species survival. Here we present data on the thermal limits of two endemic and endangered extremophile fish species, inhabiting a geothermally heated and sulfur-rich spring system in southern Mexico: The sulfur molly (Poecilia sulphuraria) and the widemouth gambusia (Gambusia eurystoma). Besides physiological challenges induced by toxic hydrogen sulfide and related severe hypoxia during the day, water temperatures have been previously reported to exceed those of nearby clearwater streams. We now present temperature data for various locations and years in the sulfur spring complex and conducted laboratory thermal tolerance tests (CTmin and CTmax) both under normoxic and severe hypoxic conditions in both species. Average CTmax limits did not differ between species when dissolved oxygen was present. However, critical temperature (CTmax=43.2°C) in P. sulphuraria did not change when tested under hypoxic conditions, while G. eurystoma on average had a lower CTmax when oxygen was absent. Based on this data we calculated both species' thermal safety margins and used a TDT (thermal death time) model framework to relate our experimental data to observed temperatures in the natural habitat. Our findings suggest that both species live near their thermal limits during the annual dry season and are locally already exposed to temperatures above their critical thermal limits. We discuss these findings in the light of possible physiological adaptions of the sulfur-adapted fish species and the anthropogenic threats for this unique system.
Exercise as Medicine for People with a Substance Use Disorder: An ACSM Call to Action Statement
Authors: Nock Nora L. , Stoutenberg Mark , Cook Dane B. , Whitworth James W. , Janke E. Amy , Gordon Adam J. 
Published: February 2024

Abstract
Over 20 million Americans are living with a substance use disorder (SUD) and nearly 100,000 die annually from drug overdoses, with a majority involving an opioid. Many people with SUD have co-occurring chronic pain and/or a mental health disorder. Exercise is a frontline treatment for chronic pain and is an effective strategy for reducing depression and anxiety and improving overall mental health. Several studies have shown that exercise improves SUD-related outcomes including abstinence; however, there is limited large-scale randomized clinical trial evidence to inform integration of exercise into practice. In this Call to Action, we aim to raise awareness of the specific issues that should be addressed to advance exercise as medicine in people with SUD including the challenges of co-occurring chronic pain, mental illness, and cardiopulmonary health conditions. In addition, specialized training for exercise professionals and other support staff should be provided on these issues, as well as on the multiple dimensions of stigma that can impair engagement in treatment and overall recovery in people with SUD.
Vaccinations in Pregnancy. A Comprehensive Overview
Authors: Plewniok Ines , Maj-Dziedzic Monika , Sikora Marcelina , Zarzycka Marta , Brzozowska Anna , Dubiel Jeremiasz , Maj Adrian , Warno Martyna , Kozik Wiktor , Śmietana Greta 
Published: February 2024

Introduction and purpose
Pregnancy is associated with many changes in the mother's body, allowing for the proper development of the fetus, but unfortunately it also predisposes many potentially harmless infections to become much more severe during pregnancy. Therefore, vaccinations during pregnancy play a very important protective role both for the mother, her unborn child and to the child immediately after birth. Immunization during pregnancy and in the preconception period protects against many infectious diseases and related complications. By receiving the vaccine, specific antibodies are synthesized and transported across the placenta to the fetus. They protect the baby during the first months of life, when the baby cannot receive vaccinations due to its age yet. Vaccinations during pregnancy can be divided into necessary, possible and recommended. Recommended vaccinations include against diphtheria, tetanus, pertussis, influenza, Covid19 and RSV. They are especially recommended for every pregnant woman who has no permanent or temporary contraindications. A special group of vaccinations are those necessary to perform, which play the main role as post-exposure prophylaxis and must be administered immediately after exposure to the agent causing the infection. These include mainly vaccination against tetanus and rabies. The vaccinations that can be performed include those targeted at a specific group of patients with various chronic diseases who are at risk or traveling to countries where there is an increased risk of illness. During a doctor's visit to a woman planning a pregnancy, it is important to collect a detailed interview regarding vaccination history, past infectious infections and chronic diseases. This allows us to prepare an individualized vaccination schedule recommended both before and during pregnancy.