bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of superior agricultural sector commodities to increase economic growth in Sumenep District
Authors: Maflahah I , Utomo S Joko , Asfan D Farida , As Fathor 
Published: January 2025

Abstract
Economic growth can be increased according to the natural potential of a region. Sumenep district has relatively high agricultural potential. Economic improvement must be made wisely, especially in determining superior agricultural commodities. This study aims to analyze the leading commodities in food crop agricultural commodities and the classification of economic growth in Sumenep District. The method approach used is Location Quotient and Shift Share analysis. The data is on the food crop production (paddy, corn, soybeans, and sweet potatoes) per subdistrict in Sumenep District in 2020 - 2023. The analysis shows that the distribution of superior paddy commodities in Sumenep District is in Batuan, Lenteng, Manding, Gapura, Batang-Batang, Raas, and Sapeken Districts. Corn commodities are in Pragaan, Bluto, Saronggi, Giligenting, Talango, Kalianget, Lenteng, Ganding, Pasongsongan, Ambunten, Rubaru, Dasuk, Batuputih, Gayam, Raas, Kangean, and Masalembu Subdistricts. The superior commodity of sweet potato was spread in Pragaan, Saronggi, Giligenting, Kalianget, Batuan, Lenteng, Rubaru, Dasuk, Batuputih, Gapura, Batang-Batang, Dungkek, Nonggunong, Gayam, Raas, and Masalembu Subdistricts.
Development of Food Seasoning using Sub-standard Fish Cracker, Pumpkin (Cucurbita moschata) and Seaweed (Kappaphycus alvarezii)
Authors: Hoy J M , Hamid M A , Ayub M S , Suratman R , Effendi M M , In’am A 
Published: January 2025

Abstract
The study focuses on sub-standard fish crackers (amplang) that do not meet quality standards but are sold at reduced prices which affecting business profitability. It aims to create a seasoning powder using sub-standard fish crackers, pumpkin (C. moschata), and seaweed (K. alvarezii). Six formulations with varying proportions of sub-standard fish cracker (55% and 65%), pumpkin powder (20%) and seaweed powder in proportions of 1.5%, 3%, and 4.5% were developed and evaluated for physicochemical properties and sensory acceptance. 65% addition of sub-standard fish cracker to the seasoning powder produced a brighter colour. Formulation (F5) with 65% sub-standard fish cracker and 3% seaweed powder obtained the highest acceptance. F5 exhibited improved nutritional quality with higher ash, protein and dietary fibre content compared to control. The seaweed powder also helped to improve the seasoning powder’s mineral profile, which is low in sodium and high in potassium. An eight-week storage study on F5 showed stable properties throughout, including water activity, colour, peroxide value, free fatty acid and microbiological status. Overall, the study offers a strategic approach to enhance the utilization of sub-standard fish crackers, improving acceptability and nutritional value for consumers.
Electric agriculture hand tractor conversion kits: a technological design, performance analysis, and innovative conversion techniques
Authors: Budiprasojo A , Tyagita D A , Rofii A 
Published: January 2025

Abstract
The shift to electric tractors is vital for sustainable agriculture, yet developing efficient conversion kits for two-wheeled tractors presents technical challenges in battery technology, motor performance, and system integration. This study aims to synthesize recent advancements in electric tractor conversion kits, focusing on technological designs, performance metrics, and innovative conversion methods using recent literature, deployed based on required formulas. Key parameters evaluated include battery energy density, motor torque, and efficiency of power control systems. High-capacity lithium iron phosphate (LiFePO4) 72V 100Ah batteries offer energy densities up to 200 Wh/kg and rapid charging rates of 1C. Electric motors max power 5kW (6,7 HP). Sustainable for agricultural applications deliver up to max 150 Nm or 19 Nm of torque at low speeds, with power densities of 3 kW/kg. Advanced power control systems employ adaptive algorithms, achieving 80% efficiency in dynamic power distribution. Electric tractor conversion kits demonstrate substantial improvements in efficiency and performance. The integration of high-performance batteries, robust motors, and sophisticated power control systems offers a viable path toward enhanced sustainability in agriculture. The adaptive power control system optimized performance across various operational conditions.
Formulation And Characterization Of Polychar Plus Granule Organic Fertilizer For Agronomic Testing On Tobacco Plants (Nicotiana tabacum L.)
Authors: Cahyaningrum DG , Sugiyarto  , Wilujeng EDI , Sulistyono NBE , Soelaksini LD , Adnyani NNP 
Published: January 2025

Abstract
Polychar plus granule was manufactured in granulated form from biochar that enriched with beneficial fungi act as decomposers for operational convenience. The preceding research substantiates the potential of utilizing organic materials, specifically coconut shells, for the production of biochar in Jember to augment plant growth. The study encompassed the formulation of a composite comprising coconut shell biochar and sheep manure compost, integrated with a decomposer containing Trichoderma harzianum DT 38, T. pseudokoningii DT 39, Aspergillus sp, and white rot fungi. The resultant product, denominated. The investigation centered on tobacco plants, the principal commodity of Jember Regency, and was conducted from May to September 2024 using a randomized block design featuring two variables: Polychar Plus Granule Dosage (G) (0.00; 12.5; 25.0; 37.5 gr/kg soil) and anorganic fertilizer dosage (P) (0;25;50;100)% from standard operating procedures (S0P). Data analysis was executed utilizing ANOVA and DMRT at a 5% significant level. The findings revealed that the application of anorganic fertilizer doses significantly influenced leaf width but yielded insignificant effects on leaf length and count. Conversely, the application of polychar plus granules showed an insignificant effect, thus requiring dose recombination in further research.
Aplication of Humic Acid and Fulvic Acid on the Growth of Chrisant (Chrysanthemum cinerariaefolium) Plants
Authors: Rohman HF , Sukri MZ , Azizah M , Putra DE , Prasetyo H , Fadilah AR 
Published: January 2025

Abstract
One of the popular houseplants in Indonesia, Chrysanthemum flowers offer great prospects for use as vase flowers, room decorations, and bouquets. However, Chrysanthemum cultivation still faces challenges in increasing fertilization efficiency through proper soil fertility management and improving the growth and quality of cutting Chrysanthemum flowers. Adding humic acid and fulvic acid, which can improve plant metabolism and soil fertility, is one method to improve fertilization efficiency. This research aimed to assess the effect of humic acid and fulvic acid on the growth of cutting chrysanthemum plants. The research was carried out at Teaching Factory Nursery and Cut Flowers, Politeknik Negeri Jember, from May to December 2024, with an altitude of ± 650 masl and a temperature of 18-25°C. We employed a Completely Randomized Design (CRD) research design, which included nine treatment combinations: control, which did not include humic and fulvic acids, humic acid at 500, 1000, 1500, and 2000 ppm, and fulvic acid at 500, 1,000, 1500, and 2000 ppm, which we repeated three times. Each experiment unit consisted of 100 plants with 30 observation samples. The study observed two key parameters: the height of the plants and the number of leaves. The analysis showed that the deployment of fulvic acid and humic acid at certain concentrations had a considerable effect on the growth of chrysanthemums. In particular, fulvic acid at a concentration of 500 ppm increased plant height during the early stages of growth, while humic acid at a concentration of 1500 ppm increased leaf number over a longer period of growth.
The Effect of Using Solid Decanter Bioconversion Products on the Performance of Laying Hens in the Pullet Period
Authors: Hasanuddin Asriani , Rusdi Rusdi , Gobel Minarny , Fatmawati Fatmawati 
Published: January 2025

Abstract
A study aimed at looking at the effect of using palm mud (solid decanter) as a bioconversion product on the performance of laying hens during the pullet period. Two hundred pullet laying hens were reared for 18 weeks, given 5 levels of bio converted palm sludge, namely; R0 (control treatment without using bio converted palm sludge); R1 (use of 6% bio converted palm sludge; R2 (use of 12% bio converted palm sludge; R3 (use of 18% bio converted palm sludge) and R4 (use of 24% bio converted palm sludge). Rations were mixed with iso protein and iso calories at 18% of protein. Five treatments and four replications were used in the research design, resulting in 20 experimental units with ten laying hens each. The research findings indicated that while the treatments had no significant effect on feed conversion ratio and ration intake (P>0.05), they had a substantial impact on egg production (HDP), egg mass, and income over feed cost (IOFC) (P<0.05). One bird in R1 group died during experimental period. In coclusion, the use of bio converted palm sludge waste (solid decanter) could be included in the ration up to 24% and can support the production performance of laying hens.
Palatability response test of hydroponic fodder (Oryza sativa, Zea mays, and Vigna radiata) in thin-tailed sheep (Ovis aries)
Authors: Kusuma S B , Wulandari S , Bahariawan A , Respati A N , Adhyatma M , Ruswanto B N Z 
Published: January 2025

Abstract
This study aims to determine the effect of plant type on palatability response in thin-tailed sheep. This study used a completely randomized design. The factor is the type of plant consisting of rice (Oryza sativa), corn (Zea mays), and green beans (Vigna radiata). Each treatment was repeated 4 times. Palatability response variables measured included grasping time (seconds), consumption amount/minute (g) and consumption time/100g (minutes). Data were analyzed using Analysis of Variety (ANOVA) and differences between means were analyzed using Duncan’s Multiple Range Test (DMRT). The results showed that different plant species had no significant effect (P>0.05) on grasping time and consumption time. However, mung bean hydroponic feed showed a significant difference (P<0.05) in the number of consumption/minutes compared to corn and rice. The conclusion of this study is that mung bean hydroponic feed has the best palatability, seen from the amount of consumption/minute which is 83 ± 8.12 g/minute. However, it is still necessary to further study the production capacity and price per kg as feed to achieve feed efficiency.
Feasibility and potential impact of a local proficiency testing program in Accra, Ghana
Authors: Ahmad Yembur , Sarpong Kwabena A N , Bansah Lois Y , Adafia Ruth , Asare Felix , Amukele Timothy K 
Published: January 2025

Abstract

Objectives
To implement a pilot proficiency testing (PT) program in Accra, Ghana, using locally produced PT materials and to explore the relationship between laboratory test costs and laboratory quality in Accra, Ghana.

Methods
Remnant serum samples from a local laboratory were pooled, aliquoted, and distributed to a convenience sample of 23 laboratories in Accra, Ghana, 2 of which had International Organization for Standardization (ISO) accreditation. One of the ISO-accredited laboratories was designated as the reference/target, and the range for passing was based on international criteria. Test cost, test results, and testing instruments used were compiled.

Results
Of the 23 laboratories, 18 submitted results. Total testing costs ranged from 80 to 312 Ghanaian cedi (GH₵) (7-26 USD). Overall accuracy (pass rate) was calculated per laboratory and per analyte. The mean laboratory accuracy was 61% (15%-92%). The pass rate for individual analytes ranged from 18% to 94% (mean, 72%). There was no correlation between test cost and pass rate.

Conclusions
The pass rates of clinical laboratories in Accra, Ghana, varied from 15% to 92%, and there was no relationship to test cost. A PT program to objectively evaluate each laboratory’s performance is needed. Making the PT material locally, as in this study, is a financially sustainable approach.
PTTM: dissecting the profile of tumor tissue microbiome to reveal microbiota features and associations with host transcriptome
Authors: Yao Ruiqian , Sun Lu , Gao Ruifang , Mei Yue , Xue Geng , Yu Dong 
Published: January 2025

Abstract
Microbiota is present in the human tissue microenvironment and closely related to tumorigenesis and treatment. However, the landscape of tissue microbiome and its relationship with tumors remain less understood. In this study, we re-analyzed the omics data from the 7104 samples (94 projects for 15 cancers) in the NCBI database to obtain microbial profiles. After normalization and decontamination processing, we established classification models to distinguish between different tumors and tumor with adjacent normal tissues. The models had excellent performances, indicating that tissue microbiome had significant tumor specificity. Moreover, a series of key bacteria and bacteria-gene association pairs were screened out based on bioinformatic analysis, such as the tumor-promoting bacteria Fusobacterium, the tumor-suppressing bacteria Actinomyces, and the significant Rhodopseudomonas-COL1A1 association pair. In addition, we created a visual website, PTTM (http://198.46.152.196:7080/), for users to query and download the results. The identified key bacteria and association pairs provide candidate targets for further exploration of the molecular mechanisms of microbial action on tumorigenesis and the development of cancer therapy.
Dose escalation pre-clinical trial of novel DOK7-AAV in mouse model of DOK7 congenital myasthenia
Authors: Cossins Judith , Kozma Imre , Canzonetta Claudia , Hawkins Al , Beeson David , Sepulveda Patricio , Dong Yin Yao 
Published: January 2025

Abstract
Congenital myasthenic syndromes are a group of inherited disorders characterized by defective neuromuscular transmission and fatigable muscle weakness. Causative mutations have been identified in over 30 genes, including DOK7, a gene encoding a post-synaptic protein crucial in the formation and stabilization of the neuromuscular junction. Mutations in this gene are one of the leading three most prevalent causes of congenital myasthenia in diverse populations across the globe. The majority of DOK7 congenital myasthenic patients experience varying degrees of disability despite receiving optimized treatment (usually salbutamol), necessitating the development of improved therapeutic approaches. Here, we executed a dose escalation pre-clinical trial using a DOK7 congenital myasthenic syndrome mouse model to assess the efficacy of AMP-101, an innovative recombinant adeno-associated viral gene replacement therapy. This mouse model harbours a duplication in the Dok7 gene that corresponds to the mutation most commonly found in DOK7 congenital myasthenia patients, c.1124-1127dupTGCC. The model has a much more severe phenotype than patients, and lives for only a few days. AMP-101 is based on AAVrh74 and contains human DOK7 cDNA under the control of a muscle-restricted promoter. Three doses of AMP-101 (2 × 1013 vg/kg, 6 × 1013 vg/kg or 1 × 1014 vg/kg) were administered intraperitoneally at 4 days of age. We show that the two higher doses of 6 × 1013 vg/kg and 1 × 1014 vg/kg generated enlarged neuromuscular junctions and rescued the very severe phenotype of the model. Treated mice became at least as strong as wild-type littermates, as demonstrated by using an inverted screen hang test, a rotarod test and a grip strength test. EMG showed that the treated model mice had decrement of compound muscle action potential on repetitive nerve stimulation, which indicates defective signalling at the neuromuscular junction. However, male models treated with 1 × 1014 vg/kg showed the least decrement that was not statistically different from wild-type littermates. Western blot analysis demonstrated robust expression of DOK7 in the diaphragm and tibialis anterior muscles. These data show that AMP-101 is an effective treatment in a mouse model for DOK7 congenital myasthenia, and suggests that AMP-101 is a promising candidate to move forward to clinic trials as a gene therapy for patients.
Inference for local projections
Authors: Inoue Atsushi , Jordá Óscar , Kuersteiner Guido M 
Published: January 2025

Abstract
Inference for impulse responses estimated with local projections presents interesting challenges and opportunities. Analysts typically want to assess the precision of individual estimates, explore the dynamic evolution of the response over particular regions, and generally determine whether the impulse generates a response that is any different from the null of no effect. Each of these goals requires a different approach to inference. In this article, we provide an overview of results that have appeared in the literature in the past 20 years along with some new procedures that we introduce here.
Diagnostic yield of echocardiography in patients with palpitations: a retrospective analysis
Authors: Khalil A M , Al Badarin F A , Al Mahameed W , Khalil M E 
Published: January 2025

Abstract

Introduction
Transthoracic echocardiography (TTE) is a fundamental tool in evaluating patients with palpitations. However, diagnostic yield of TTE in this population, especially younger patients who are not previously known to have heart disease has not been adequately described. Therefore, we aimed to determine the prevalence of structural cardiac abnormalities among patients undergoing TTE for this indication.

Methods
Electronic database of all TTE studies performed at a single tertiary care center (November 2015 to December 2023) was used to identify consecutive patients undergoing TTE for the indication of palpitations alone. Studies performed for palpitations and other indications suggesting presence of structural heart disease were excluded. The primary outcome was the prevalence of any cardiac abnormality defined as any abnormality in chamber size, ventricular systolic or valvular function, or significant cardiac abnormality defined as presence of severe chamber dilation, severe valvular or systolic dysfunction. Binomial logistic regression was used to predict absence of structural abnormalities on TTE.

Results
Among 3,434 included patients, 348 (10.1%) had abnormal TTE, out of which 116 (3.4%) had significant abnormalities. Female sex, younger age, and lower BMI were independently associated with absence of all abnormalities on TTE (P&lt;.001 for all). Prevalence of any or severe cardiac abnormalities in women was 7% and 2.3%, respectively, in patients &lt;40 years was 4.1% and 0.9%, respectively, and in those with BMI&lt;30 was 7.6% and 3.1%, respectively. In a subset of women younger than 40 with BMI&lt;30 (n=887) the prevalence of any cardiac abnormality was 1.6% and of severe abnormalities was 0.9% (Figure).

Conclusion
Whereas TTE is frequently utilized for evaluation of patients with palpitations, its yield is relatively low, particularly in younger patients and those with lower BMI, especially women. A selective approach in utilizing TTE in this population is prudent, especially when guided by other clinical or electrocardiographic findings. Prospective studies may help refine the role of TTE in this context.
Using gamification tools to enhance self-organized echo-learning in a hospital setting
Authors: Trautmann N 
Published: January 2025

Abstract

Background
Gamification has been discussed in various settings of medical education, highlighting its potential to increase engagement and boost motivation. Tools like "points," "badges," or "leaderboards" are known to work well in any area of learning.

Purpose
In a mid-sized hospital, creating a structured echocardiography training curriculum for internal medicine residents can be challenging due to rotating teachers and students and a general lack of resources. A self-guided training curriculum using concepts from computer gaming seemed appropriate to engage and motivate trainees.

Methods
We set up a self-organized training program where residents without prior echocardiography skills could progress through a step-by-step curriculum to gain basic echocardiography expertise. After a brief introductory session and distribution of a paper curriculum, trainees were included in the daily echocardiography schedule. They could advance to higher "levels," each allowing them to use more advanced tools, such as spectral Doppler or the Simpson method ("weapons"). To complete each level, trainees had to answer theoretical questions, analyze echo loops stored on a network drive, and perform a short hands-on exam under the supervision of a senior cardiologist ("final boss"). We also published "highscores" regularly, highlighting residents with the most exams during a given interval. To enhance the skills of our senior cardiologists, we developed "challenges" introduced several times per year, covering techniques like lung ultrasound or pulmonary vein Doppler.

Results
The process was evaluated through an anonymous online questionnaire completed by about half of the residents. The training concept received positive feedback, but the results were overshadowed by the lack of time residents were able to allocate to perform echocardiographies at all. Additionally, the amount of in-depth personal supervision was criticized.

Conclusions
Although our echocardiography education concept did not fulfill all our expectations, developing a self-organized echocardiography training curriculum using gamification elements seems promising. Future refinements should include more supervision and regular training and briefing of all individuals involved in the process. Even more important seems to be a general allocation of resources to echocardiography training. Since medical education is vital for ensuring treatment quality and staff satisfaction, more research is necessary to test and compare different teaching approaches scientifically. In the future, an online or mobile-app based training platform could serve as a hub for echocardiography teaching, where institutions and learners register, access teaching resources, and track their progress, using gamification tools to motivate and engage trainees.
Training scheme
Challenge.jpg