bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization, characterization and biosafety of oregano, rosemary and mint oil mixture against Penicillium digitatum in citrus using L-optimal mixture design
Authors: Khaled Rahaf , Mohamed Sara , Mohamed Amira , Khairy Aya , Elhariry Hesham , Bakry Ashraf , Elsenosy Neima K. , Ebeed Naglaa , Salem Salem S. , ElNoby Thanaa , Abu-Hussien Samah H. 
Published: January 2025

Abstract
The increasing demand for natural alternatives to synthetic fungicides has prompted research into natural products like essential oils for postharvest disease management. This study investigated the antifungal, antioxidant, cytotoxic, and genotoxic potential of essential oil mixtures derived from oregano, rosemary, and mint against Penicillium digitatum, the predominant fungal pathogen causing green mold in orange fruits. P. digitatum NPAGRASU 2024 was isolated and identified as the most abundant species (60.2%) from infected oranges. It was deposited in GenBank with gene accession number PP930644 and deposited in MIRCEN culture collection as EMCC 358874. The antimicrobial activity of individual essential oils was evaluated, with oregano exhibiting the highest antifungal activity (inhibition zone diameter of 4.2 cm) against P. digitatum. L-optimal mixture design of response surface methodology (RSM) optimization revealed a highly effective mixture (Run 8) comprising 46.26% oregano and 53.74% rosemary, with a 99.65% actual growth reduction. The oregano oil demonstrated potent antioxidant activity, reaching approximately 75% DPPH radical scavenging at 3.125 mg/mL. Cytotoxicity assessment using the MTT assay showed morphological changes and reduced cell viability in liver cells treated with the PEOs mixture at 300 µg/mL. However, the optimized mixture did not induce significant chromosomal aberrations compared to the control, suggesting minimal genotoxic effects. In vivo, evaluation on oranges revealed 60% inhibition of green mold by the 1% (v/v) optimized mixture for 7 days. Histological analysis indicated low toxicity to the liver at the highest tested concentration (1% mixture). GC-MS analysis identified major compounds like cavarcol, caryophyllene, eucalyptol, phenols and levomenthol in oregano oil, contributing to its bioactivities. This study demonstrates the potential of optimized essential oil mixtures as effective and eco-friendly alternatives for postharvest disease control.
Vision impairment in boys recruited to the iREAD study
Authors: Levine Jonathan , Doron Ravid , Ostrin Lisa A. , Shneor Einat 
Published: January 2025

Abstract

Background
Uncorrected refractive error is reported to be the most common cause globally of vision impairment in school age children. However, little is known about the extent of uncorrected refractive error in Israel. The purpose of this study was to investigate the prevalence of vision impairment in schoolchildren recruited for the Israel Refraction, Environment, And Devices (iREAD) Study.

Methods
Healthy boys, ages 5–13 years, were recruited to participate in the iREAD Study. Parents first answered a questionnaire to exclude children with a known history of amblyopia, strabismus, or hyperopia. A comprehensive eye exam was then performed. Presenting visual acuity < 6/12 was defined as vision impairment. Myopia and hyperopia were defined as cycloplegic spherical equivalent refraction  ≤ − 0.50 D, and ≥  + 0.50 D, respectively, and astigmatism as ≤ − 0.75 D. Amblyopia was defined as best corrected visual acuity ≤ 6/12 in at least one eye in the absence of any ocular pathology. Descriptive statistics were used to calculate the prevalence of each refractive error and amblyopia.

Results
Two hundred five boys (average age 8.8 ± 1.7 years) presented for a comprehensive eye exam. The prevalence of vision impairment at initial presentation was 22.9% (N = 47), with 16.1% (N = 33) and 6.8% (N = 14) for both eyes and one eye, respectively. Of the children with vision impairment, 36.2% (N = 17) were wearing habitual correction. Of the children with vision impairment, 97.9% (N = 46) had refractive error, with 85.1% (N = 40) being myopic and 12.8% (N = 6) being hyperopic. In addition, 36.2% (N = 17) with vision impairment had astigmatism. Most children with vision impairment (N = 43) achieved good vision with refractive correction. However, amblyopia was observed in 2.0% (N = 4) of the children.

Conclusions
A high prevalence of vision impairment was observed, primarily due to uncorrected or undercorrected refractive error. Children with amblyopia and/or hyperopia presented despite a parent questionnaire to exclude children with these conditions. Findings suggest that many parents are unaware of their children’s visual and refractive status, even for children who already have glasses. In conclusion, improvements to the current system in Israel of vision screenings in first grade should be made to help insure children in need receive adequate follow-up throughout their education.
Global dynamics on a delayed double-strain influenza model with vaccination and cross-immunity
Authors: Gao Shukun , Zhang Long , Li Hongli , Teng Zhidong 
Published: January 2025

Abstract
In this paper, a delayed double-strain influenza model with vaccination and cross-immunity is proposed to explore the effect of coinfection of double-strain on disease spread. First, the nonnegativity and ultimate boundedness of solution are proved. Second, the basic reproduction numbers of strains 1, 2, and the whole model are defined respectively, by which criteria on the local and global asymptotic stability of (disease-free, dominant) equilibria are established. The uniform persistence of (strains 1, 2 coexistent) disease is obtained as well. Finally, the validity of the theoretical results is demonstrated by numerical simulations. We find that neglecting cross-immunity and vaccination would misestimate the size of influenza outbreaks. Cross-type multivalent vaccines will be the main direction for effective control measure for influenza.
Borehole heat exchangers in coastal areas may reduce heatwave seagrass loss
Authors: Criollo Rotman , Vilarrasa Víctor , Orfila Alejandro , Marbà Núria , Fernández-Mora Angels 
Published: January 2025

Abstract
Mitigation of climate change, implies, among other measures, the increase on the use of carbon-free energy sources, ensuring a zero environmental footprint. Borehole heat exchangers (BHE), a renewable heat source from the ground used for thermal regulation of infrastructures, could significantly reduce CO2 emissions. Yet, BHE perturbs groundwater temperature by heating and cooling the ground in summer and winter, respectively, which may alter the temperature of Sea Groundwater Discharges. We propose a methodology to strategically locate BHE to discharge cooler temperatures into the sea during summer, alleviating the extreme marine temperatures occurring during marine heatwaves around the groundwater discharge areas, and ensuring a thermal refuge for temperature-sensitive coastal ecosystems. This study opens a new avenue to accomplish the climate targets of the Paris Agreement and mitigate the negative effects of global warming on coastal ecosystems.
Preclinical evaluation and preliminary clinical study of 68Ga-NODAGA-NM-01 for PET imaging of PD-L1 expression
Authors: Zhao Lingzhou , Gong Jiali , Liao Sisi , Huang Wenhua , Zhao Jinhua , Xing Yan 
Published: January 2025

Abstract

Background
Programmed cell death 1/programmed death ligand-1 (PD-L1)-based immune checkpoint blockade is an effective treatment approach for non-small-cell lung cancer (NSCLC). However, immunohistochemistry does not accurately or dynamically reflect PD-L1 expression owing to its spatiotemporal heterogeneity. Herein, we assessed the feasibility of using a 68Ga-labeled anti-PD-L1 nanobody, 68Ga-NODAGA-NM-01, for PET imaging of PD-L1.

Methods
Micro-PET/CT and biodistribution studies were performed on PD-L1-positive and -negative tumor-bearing mice. Additionally, a preliminary clinical study was performed on two patients with NSCLC. NM-01 was radiolabeled with 68Ga without further purification under mild conditions.

Results

68Ga-NODAGA-NM-01 exhibited radiochemical purity (> 98%), high stability in vitro, and rapid blood clearance in vivo. Specific accumulation of 68Ga-NODAGA-NM-01 was observed in PD-L1-positive tumor-bearing mice, with a good tumor-to-background ratio 0.5h post-injection. Furthermore, 68Ga-NODAGA-NM-01 PET/CT imaging was found to be safe with no adverse events and distinct uptake in primary and metastatic lesions of the PD-L1-positive patient, with a higher maximal standardized uptake value than that in lesions of the PD-L1-negative patient 1h post-injection.

Conclusions

68Ga-NODAGA-NM-01 can be prepared using a simple method under mild conditions and reflect PD-L1 expression in primary and metastatic lesions. However, our findings need to be confirmed in a large cohort.

Trial registration
NCT02978196. Registered February 15, 2018.
MELK prevents radiofrequency ablation-induced immunogenic cell death and antitumor immune response by stabilizing FABP5 in hepatocellular malignancies
Authors: Tang Bu-Fu , Xu Wang-Ting , Fang Shi-Ji , Zhu Jin-Yu , Qiu Rong-Fang , Shen Lin , Yang Yang , Weng Qiao-You , Wang Ya-Jie , Ding Jia-Yi , Zhang Xiao-Jie , Chen Wei-Qian , Zheng Li-Yun , Song Jing-Jing , Chen Biao , Zhao Zhong-Wei , Chen Min-Jiang , Ji Jian-Song 
Published: January 2025

Abstract

Background
Radiofrequency ablation (RFA) is an efficient treatment with unlimited potential for liver cancer that can effectively reduce patient mortality. Understanding the biological process related with RFA treatment is important for improving treatment strategy. This study aimed to identify the critical targets for regulating the efficacy of RFA.

Methods
The RFA treatment in hepatocellular carcinoma (HCC) tumor models in vivo, was analyzed by RNA sequencing technology. The heat treatment in vitro for HCC tumor cells was also constructed to explore the mechanism after RFA treatment in tumor cells. Nanoparticles with high affinity to tumor cells were applied as a new therapy to interfere with the expression of maternal embryonic leucine zipper kinase (MELK).

Results
It was found that RFA treatment upregulated MELK expression, and MELK inhibition promoted RFA efficacy by immunogenic cell death and the antitumor response, including anti-tumoral macrophage polarization and increased CD8+ T cell cytotoxicity in HCC. Mechanically, MELK binds to fatty acid-binding protein 5 (FABP5), and affects its ubiquitination through the K48R pathway to increase its stability, thereby activating protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling axis to weaken the RFA-mediated antitumor effect. In addition, the synthesis of arginylglycylaspartic acid (RGD)-lipid nanoparticles (LNPs) targeting tumor cell-intrinsic MELK enhanced RFA efficacy in HCC.

Conclusion
MELK is a therapeutic target by regulating RFA efficacy in HCC, and targeting MELK via RGD-LNPs provides new insight into improving RFA efficacy in HCC clinical treatment and combating the malignant progression of liver cancer.
Ethnobiological survey on medicinal plants used by gaddi and gujjar tribes of riparian region of Beas River of Himachal Pradesh in North Western Himalayas, India
Authors: Thakur Neha , Kumar Nitesh , Kumar Sanjeev , Sharma Anju , Ahlawat Yogesh K. , Malik Anurag , Ahmed Meraj 
Published: January 2025

Abstract
Himachal Pradesh is one of the most species-rich regions in the world and a key center for the origin and diversity of numerous plant species. Ethnobotanical research is essential for exploring these diverse biological resources for medicinal uses. This study aimed to record the traditional knowledge of the tribal communities in Himachal Pradesh concerning the use of medicinal plants for treating diseases in humans and livestock. Despite this richness, there is a lack of detailed documentation about the use of therapeutic plants in this area. The indigenous communities in this region rely on traditional knowledge of medicinal plants, making it a valuable resource for further pharmacological and phytochemical research. The ethnobotanical surveys were conducted from August 2022 to July 2023, and our fieldwork took place in four districts (Kullu (1279 m), Mandi (760 m), Hamirpur (780 m) and Kangra (1200–1300 m) under various villages. The ethnobiological data was gathered through field surveys, open interviews, discussions, and semi-structured questionnaires conducted with 193 tribal participants aged 40 to 80 years. Informants were chosen using the randomized household interview and snowball sampling methods. The study documented 64 plants from 39 families, among which 58 species are angiosperms, 5 species are pteridophytes and one species is bryophyte utilized for therapeutic purposes. Rosaceae, Lamiaceae, and Ranunculaceae were the most used medicinal herb families. Rubus ellipticus (0.75) and Trillium govanianum (0.74) demonstrated the highest UV (Use value) values. The ICF (Informant Consensus factor) value revealed that nervous system disorders-related ailments were the most treated. This study explored the ethnobiological information of therapeutic flora from riparian vegetation within tribal communities while also assessing the present state of research and the practical application of their medicinal information. It underscores the potential for additional research to delve deeper into the indigenous properties of their therapeutic floras and emphasizes the importance of preserving their traditional medicinal wisdom.
Revisiting the trading activity of high-frequency trading firms around ultra-fast flash events
Authors: Desagre Christophe , Laly Floris , Petitjean Mikael 
Published: January 2025

Abstract
We investigate high-frequency traders’ behavior in the context of the fastest and most extreme price movements (EPMs) that can be observed in the market, specifically ultra-fast flash events, challenging the methodologies employed in the academic and practitioner literature for identifying sudden liquidity black holes. To refine the price-shock identification methodology, we introduce a new approach called sequence-based flash events (SFEs), which relies on tick sequences instead of predetermined fixed-time intervals within which all flash events in the sample are assumed to occur. This alternative methodology offers the advantage of pinpointing the exact time and duration of a crash, which, in turn, provides a way to more accurately define the observation windows around it. We compare our sample of SFEs with both the so-called “mini flash crashes”, as identified by the Nanex detection algorithm, and the so-called EPMs, as identified by Brogaard et al. (2018). We use close and open prices, as well as high and low prices. Based on our sample of SFEs, we find no evidence that HFTs trigger extreme price shocks. However, we find that HFTs exacerbate SFEs by increasing the net imbalance in the direction of these shocks as they occur. Finally, we show that the choice of the price-shock identification methodology is critical. Thus, we urge regulators to exercise caution and avoid hasty conclusions regarding HFTs’ contribution to price stability in stressful market conditions.
Effect of thymol and propolis extract on genotoxicity, biochemical activities and expression profile of some genes on honey bee, Apis mellifera, infected with Vairimorpha (Nosema) ceranae
Authors: Sebak Salwa I. , Elelimy Heba A. S. , Seyam Heba , Elkenawy Soad A. 
Published: January 2025

Abstract

Background

Nosema ceranae, the predominant microsporidian parasite, weakens Apis mellifera honey bee colonies and reduces their productivity and reproduction. This research aims to detect the impact of thymol and propolis extracts on genotoxicity repair, oxidative enzymes, expression of some antimicrobial genes and some hypopharyngeal glands genes in N. ceranae-infected bees. Experimentally, infected honeybee colonies were divided into four groups: (1) untreated, (2) for propolis (3 g/L) treatment, (3) for thymol (0.1 g/L) treatment and (4) for mixed treatment with thymol and propolis. Workers from adult honeybee colonies, treated and untreated, were randomly sampled. The collected samples were used in studying the genotoxicity effect, the concentration of oxidative enzymes (superoxide-dismutases, glutathione S transferase, catalase and malondialdehyde (MDA)), measuring the relative expression of antimicrobial genes and hypopharyngeal glands genes major royal proteins 1 & 2 (MJRP1 and MJRP2).

Results
The results show that thymol and propolis extract decreased genotoxicity effect, increased expression of antimicrobial genes, increased expression levels of MJRP1 and MJRP2 genes and decreased activities of oxidative enzymes when applied to Nosema-infected bees.

Conclusion
Thymol and propolis extract positively affect honey bee health and mix of them. This study reveals that natural product extracts and their mixture may fight Nosema and prevent honey bee colonies decline and sudden death.
Danger of co-administration ferric carboxymaltose and denosumab: hypophosphatemia
Authors: Seker Aysun , Durmus Nurdan Senturk 
Published: January 2025

Abstract
Both denosumab-induced hypocalcemia and ferric carboxymaltose infusion-related hypophosphatemia have been extensively documented. There are significant risks associated with combining ferric carboxymaltose parenteral infusions and denosumab, including potentially life-threatening electrolyte imbalances such as hypocalcemia and hypophosphatemia. Developing monitoring strategies for electrolyte abnormalities can be facilitated by the increased awareness of this interaction. We present the case of a 62-year-old female patient who developed severe, symptomatic hypocalcemia and hypophosphatemia despite having normal renal function. These abnormalities occurred concomitantly during treatment with denosumab for osteoporosis and ferric carboxymaltose for iron deficiency anemia.
Associations between IL-6 and trajectories of depressive symptoms across the life course: Evidence from ALSPAC and UK Biobank cohorts
Authors: Edmondson-Stait Amelia J. , Davyson Ella , Shen Xueyi , Adams Mark James , Khandaker Golam M. , Miron Veronique E. , McIntosh Andrew M. , Lawrie Stephen M. , Kwong Alex S.F. , Whalley Heather C. 
Published: January 2025

Abstract

Background
Peripheral inflammatory markers, including serum interleukin 6 (IL-6), are associated with depression, but less is known about how these markers associate with depression at different stages of the life course.

Methods
We examined the associations between serum IL-6 levels at baseline and subsequent depression symptom trajectories in two longitudinal cohorts: ALSPAC (age 10–28 years; N = 4,835) and UK Biobank (39–86 years; N = 39,613) using multilevel growth curve modeling. Models were adjusted for sex, BMI, and socioeconomic factors. Depressive symptoms were measured using the Short Moods and Feelings Questionnaire in ALSPAC (max time points = 11) and the Patient Health Questionnaire-2 in UK Biobank (max time points = 8).

Results
Higher baseline IL-6 was associated with worse depression symptom trajectories in both cohorts (largest effect size: 0.046 [ALSPAC, age 16 years]). These associations were stronger in the younger ALSPAC cohort, where additionally higher IL-6 levels at age 9 years was associated with worse depression symptoms trajectories in females compared to males. Weaker sex differences were observed in the older cohort, UK Biobank. However, statistically significant associations (pFDR <0.05) were of smaller effect sizes, typical of large cohort studies.

Conclusions
These findings suggest that systemic inflammation may influence the severity and course of depressive symptoms across the life course, which is apparent regardless of age and differences in measures and number of time points between these large, population-based cohorts.
Surgical treatment results for oligo-recurrence of gastric cancer.
Authors: Jang Youjin JIN 
Published: January 2025

462

Background:
Despite advancements in diverse treatments, approximately 40-50% of advance gastric cancer patients experience recurrence even after curative resection surgery. The condition where there is a limited recurrence after the control of the primary tumor site is referred to as oligo-recurrence. However, there is scarce reporting on oligo-recurrence after gastric cancer surgery. This study compiled cases of patients who underwent surgery for recurrent gastric cancer after R0 surgery. Since there are no specific treatment guidelines for recurrence after gastric cancer surgery, this research could be beneficial in providing insights and assistance.
Methods:
This retrospective study includes data from 25 patients who underwent surgery for suspected oligo-recurrence of gastric cancer among those who had undergone surgery from June 2008 to May 2018. If a solitary mass suspected of recurrence is identified during surveillance, a PET-CT is performed. Patients with suspicion of peritoneal dissemination, carefully excluded through a multidisciplinary approach, are selectively omitted. All patients undergo excision surgery for both treatment and tissue examination purposes.
Results:
Out of 25 cases, there were 6 cases of paraaortic lymph node enlargement, 9 cases liver nodules, 8 case lung nodules, and 1 case tumor bed mass. In the postoperative tissue analysis, 4 cases of paraaortic lymph node enlargement, 5 cases of the liver nodules, and 1case tumor bed mass were confirmed to be recurrences of gastric cancer. All of lung nodule were diagnosed with lung cancer. All 10 cases underwent chemotherapy after metastatectomy. Among them, 3 cases experienced recurrence and died an average of 12.3 months after surgery. Seven cases did not experience recurrence and have survived with an average survival time of 102 months to date.
Conclusions:
The metastatectomy is safe and effective for patients with oligo-recurrence from GC and can improve their prognosis. For an accurate diagnosis, oligometastectomy is essential. However, our results need to be confirmed by more randomized controlled clinical studies.

Treatment result after metastatectomy.

Case
Diagnosis
Chemotherapy aftermetastatectomy
Recurence after metastatectomy
DFS after metastatectomy
OS after metastatectomy
OS after gastrectomy
Status

1
LN metastasis 1/1
15cycles FOLFOX

147
147
156.4
ALIVE

2
LN metastasis 1/1
37cycles TAS-118
liver metastasis
22
40.6
74.9
DEATH

3
LN metastasis 1/3
12cycles FOLFOX

98.1
98.1
125.5
ALIVE

4
LN metastasis 1/1
35cycles FOLFOX

50.9
50.9
71.3
ALIVE

5
Liver metastasis
ONO tiral
Liver, bone mets.
3.73
27.9
64.6
DEATH

6
Liver metastasis
12cycles FOLFOX

68.3
68.3
122.2
ALIVE

7
Liver metastasis
12cycles FOLFOX

96
96
105.8
ALIVE

8
Liver metastasis
82cycles FOLFOX

55
55
77.4
ALIVE

9
Liver metastasis
12cycles FOLFOX

94.4
94.4
103.9
ALIVE

10
Tumor bed
12cycles FOLFOX

9
25.5
24.4
DEATH
Dendritic cells in developing and adult zebrafish arise from different origins and display distinct flt3 dependencies
Authors: Lin Guanzhen , Wang Youqi , Pham Thi Giang , Wen Zilong 
Published: January 2025

ABSTRACT
Dendritic cells (DCs) are key cellular components of the immune system and perform crucial functions in innate and acquired immunity. In mammals, it is generally believed that DCs originate exclusively from hematopoietic stem cells (HSCs). Using a temporal-spatial resolved fate-mapping system, here we show that, in zebrafish, DCs arise from two sources: dorsal aorta-born endothelium-derived hematopoietic progenitors (EHPs) and HSCs. The EHP-derived DCs emerge early, predominantly colonizing the developing thymus during larval stages and diminishing by juvenile stages. In contrast, HSC-derived DCs emerge later and can populate different tissues from late larval stages to adulthood. We further document that the EHP- and HSC-derived DCs display different dependencies on Fms-like tyrosine kinase 3 (Flt3), a pivotal receptor tyrosine kinase crucial for DC development in mammals. Our study reveals the presence of two distinct waves of DC development in zebrafish, each with unique origins and developmental controls.