bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical Fabrication of Pd–Au Heterogeneous Nanocontact Showing Stable Conductance Quantization under Applying High Bias Voltage
Authors: Li Jingze , Nakato Yoshihiro , Murakoshi Kei 
Published: February 2005

Abstract
A novel nanostructure, heterogeneous Pd–Au nanocontact showing conductance quantization, was fabricated via selective electrochemical deposition of Pd at a gap of two sustainable thin Au wires in solution. The stability of Pd monatomic contact increased when the potential of the electrode was kept at negative potential. Contribution of the adsorbed hydrogen on Pd–Au contact was considered as the origin of the improved stability.
First Example of Cationic Ring-Opening Polymerization of γ-Thionobutyrolactone
Authors: Kikuchi Hirofumi , Tsubokawa Norio , Endo Takeshi 
Published: February 2005

Abstract
This paper deals with the cationic ring-opening polymerization of γ-thiobutyrolactone (2) and γ-thionobutyrolactone (3). The ring-opening polymerization of 3 was carried out in bulk at 100 °C for 2 h by various cationic initiators. Scandium trifuoromethane-sulfonate induced the polymerization of 3, which did not afford polythionoester but polythioester selectively. On the other hand, the polymerization of 2 did not proceed with various cationic initiators under the similar conditions.
Solution of a SAT Problem on a Photochemical DNA Computer
Authors: Ogasawara Shinzi , Fujimoto Kenzo 
Published: February 2005

Abstract
The photochemical DNA computing via 5-carboxyvinyl-deoxyuridine (cvU) in anchor oligodeoxynucleotides (ODNs) in order to tether the multiple “DNA words” was demonstrated. A new MARK and UNMARK operation based on the cvU mediated reversible DNA photoligation has been developed for multiple-words DNA computing. The utility of this operation for DNA computing was demonstrated by solving a satisfiability problem (SAT problem) in which information was encoded in three tandem words.
X-ray Absorption Fine Structure Studies on the Local Structures of Ni Impurities in a Carbon Nanotube
Authors: Asakura Kiyotaka , Chun Wang-Jae , Tohji Kazuyuki , Sato Yoshinori , Watari Fumio 
Published: February 2005

Abstract
Local structure around Ni impurities in a carbon nanotube was studied by X-ray absorption fine structure (XAFS). The Ni was present in the form of Ni particles before the purification process. After the purification Ni content was decreased to a few hundreds ppm. The Ni K-edge XAFS could be measured by a 19 element solid-state detector. The Ni species was strongly bound to the carbon of the carbon nanotube with a Ni–C covalent bonds at 0.173 nm.
Fabrication of Polyaniline/Ethylcellulose Composite Microspheres by Microencapsulation
Authors: Yang Fuyong , Chu Ying , Huo Lei , Yang Yang , Liu Yang 
Published: February 2005

Abstract
Polyaniline/ethylcellulose composite microspheres were prepared through microencapsulation technique via a modified emulsion–solvent evaporation method. The core was gained through the polymerization of aniline, while the shell was formed by the solidification of ethylcellulose under the condition of the evaporation of organic solvent.
Synthesis and CO Oxidation Catalytic Character of High Surface Area Ruthenium Dioxide Replicated by Cubic Mesoporous Silica
Authors: Shen Weihua , Shi Jianlin , Chen Hangrong , Gu Jinlou , Zhu Yufang , Dong Xiaoping 
Published: February 2005

Abstract
A high surface area ruthenium dioxide with partial ordered structure has been replicated by cubic Ia3d mesoporous silica, and the replica shows high catalytic activity for the oxidation of CO to CO2 at relatively low temperature.
Organic Field-effect Transistor Based on Biphenyl Substituted TTF
Authors: Noda Bunpei , Katsuhara Mao , Aoyagi Isao , Mori Takehiko , Taguchi Tomohiro , Kambayashi Takuya , Ishikawa Ken , Takezoe Hideo 
Published: February 2005

Abstract
Tetrathiafulvalene-based organic semiconductor with biphenyl substituents, DBP-TTF (bis(biphenyl)tetrathiafulvalene) is used as an active layer of an organic field-effect transistor. DBP-TTF forms a good thin film on a SiO2 substrate, in which the DBP-TTF molecules are arranged almost perpendicular to the substrate. The field-effect mobility of the thin-film transistor amounts to 0.11 cm2V−1s−1.
Synthesis of Highly Ordered Mesoporous Tantalum Oxide
Authors: Nakajima Kiyotaka , Hara Michikazu , Domen Kazunari , Kondo Junko N 
Published: February 2005

Abstract
The pore arrangement of mesoporous tantalum oxide prepared with a block copolymer surfactant as a structure-directing agent is remarkably improved by optimizing the amounts of metal source and water content. In contrast to the wormhole-like pore structure observed in the mesoporous tantalum oxide prepared under standard conditions, a highly ordered mesoporous structure is formed under optimized conditions.
Controllable Synthesis of One-dimensional Single-crystalline TiO2 Nanostructures
Authors: Wen Baomei , Liu Chunyan , Liu Yun 
Published: February 2005

Abstract
One-dimensional (1-D) TiO2 nanostructures (nanoribbons, nanorods, nanotubes) were obtained by a simple solvothermal process in the presence of organic solvents in which the resulting products were basically single crystals. Morphologies of single crystalline TiO2 were successfully controlled through the optimization of various solvents.
Effective One-pot Synthesis of H type 1 and 2 Trisaccharide Derivatives Using Glycal Epoxide
Authors: Tanaka Hiroshi , Matoba Nobuatsu , Takahashi Takashi 
Published: February 2005

Abstract
We describe an efficient synthesis of H type 1 and 2 trisaccharides by one-pot glycosylation involving glycosidation of glycal epoxide.
Formation of a Ligand-based Mixed-valence Cluster Triggered by Dehydration Condensation of Semiquinonates with o-Phenylenediamines
Authors: Chang Ho-Chol , Nishida Nao , Kitagawa Susumu 
Published: February 2005

Abstract
A tetrameric [CoII(phenSQ)2]4 with homovalent semiquinonates reacts with o-phenylenediamines to give a ligand-based mixed-valence pentanuclear cluster [{CoII5(phenSQ)4(phenCat)3(THF)3(H2O)}·THF], containing two differently charged ligands, whose formation is triggered by a dehydration condensation reaction between the semiquinonates and o-phenylenediamines.
One Pot Synthesis of Isometrically Pure 5-Carboxy-sulforhodamines and Their Application for Labeling Proteins
Authors: Wang Zhi-Qiang , Diwu Zhenjun , Francisco-Reyes Jeannie , Yi George G 
Published: February 2005

Abstract
New reactive fluorescent dyes, 5-carboxy-sulforhodamines, were synthesized by one pot synthesis and their labeling proteins were examined.
Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients
Authors: Douard Richard , Moutereau Stéphane , Serru Valérie , Sales Jean Patrick , Wind Philippe , Cugnenc Paul-Henri , Vaubourdolle Michel , Loric Sylvain 
Published: February 2005

AbstractCirculating cell detection using reverse transcriptase-polymerase chain reaction (RT-PCR) techniques has been studied as a new prognostic factor in colorectal cancer patients. With the view of enhancing detection sensitivity, we developed a new multiplex RT-PCR assay for circulating cell detection based on the expression of carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5; formerly CEA) and CEACAM7 (formerly CGM2).Between November 2002 and December 2003, 45 stage III-IV, 39 stage I-II colorectal cancer patients, 32 non-colorectal cancer patients and 41 healthy individuals were included. Positive selection using HEA-125 immunobeads was applied to blood samples before mRNA extraction, cDNA synthesis and a multiplex CEACAM5/CEACAM7 RT-PCR assay. For both CEACAM5 and CEACAM7, the limit of detection was found to be as low as 1 expressing cell in 10The multiplex RT-PCR assay was negative for the 41 healthy individuals and the 32 non-colorectal cancer patients. The test was positive in 53/84 (63%) of the colorectal cancer patients for CEACAM5 and/or CEACAM7, whereas 32/84 (38%) were positive for both markers. Colorectal cancer patients were positive for one of the two markers in 80% of cases (36/45) for stage III-IV patients (CEACAM5 73%, CEACAM7 51%) and in 44% of cases (17/39) for stage I-II patients.This multiplex RT-PCR assay with two markers proved to be more sensitive than use of a single marker in detecting circulating tumour cells. The discrepant expression of CEACAM5 and CEACAM7 may label circulating tumour cells that have different levels of differentiation and subsequent aggressive behaviour.
Modulation of translation factor's gene expression by histone deacetylase inhibitors in breast cancer cells
Authors: Gonçalves João , Malta-Vacas Joana , Louis Monette , Brault Laurent , Bagrel Denyse , Monteiro Carolino , Brito Miguel 
Published: February 2005

AbstractThe histone deacetylase inhibitors sodium butyrate (NaBu) and trichostatin A (TSA) exhibit anti-proliferative activity by causing cell cycle arrest and apoptosis. The mechanisms by which NaBu and TSA cause apoptosis and cell cycle arrest are not yet completely clarified, although these agents are known to modulate the expression of several genes including cell-cycle- and apoptosis-related genes. The enzymes involved in the process of translation have important roles in controlling cell growth and apoptosis, and several of these translation factors have been described as having a causal role in the development of cancer. The expression patterns of the translation mechanism, namely of the elongation factors eEF1A1 and eEF1A2, and of the termination factors eRF1 and eRF3, were studied in the breast cancer cell line MCF-7 by real-time quantitative reverse transcription-polymerase chain reaction after a 24-h treatment with NaBu and TSA. NaBu induced inhibition of translation factors' transcription, whereas TSA caused an increase in mRNA levels. Thus, these two agents may modulate the expression of translation factors through different pathways. We propose that the inhibition caused by NaBu may, in part, be responsible for the cell cycle arrest and apoptosis induced by this agent in MCF-7 cells.
Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification
Authors: Thompson Miles D. , Bowen Raffick A. R. , Wong Betty Y. L. , Antal Joan , Liu Zhanqin , Yu Helen , Siminovitch Katherine , Kreiger Nancy , Rohan Tom E. , Cole David E. C. 
Published: February 2005

AbstractWhile buccal cells provide an easily accessible source of genomic DNA, the amount extracted may be insufficient for many studies. Whole genome amplification (WGA) using multiple displacement amplification (MDA) may optimize buccal cell genomic DNA yield. We compared the usefulness, in epidemiological surveys, of DNA derived from buccal cells collected by alcohol mouthwash and amplified by WGA protocol and standard protocols. Buccal cell collection kits were mailed to 300 randomly selected members of a large cohort study, and 189 subjects returned buccal cell samples. We determined: (i) which QIAamp
Y-Chromosome short tandem repeat (STR) haplotypes in a Campania population sample
Authors: Calcagno Giuseppe , Labruna Giuseppe , Sacchetti Lucia 
Published: February 2005

AbstractGiven the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex reactions: multiplex A for loci
TaqMan assays for genotyping of single nucleotide polymorphisms present at a disease susceptibility locus on chromosome 6
Authors: Koch Werner , Hoppmann Petra , Michou Elena , Jung Vanessa , Pfeufer Arne , Müller Jakob , Meitinger Thomas , Schömig Albert , Kastrati Adnan 
Published: February 2005

AbstractMultiple single-nucleotide polymorphisms in the
The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis
Authors: Levreri Isabella , Caruso Ubaldo , Deiana Franca , Buoncompagni Antonella , De Bernardi Bruno , Marchese Nora , Melioli Giovanni 
Published: February 2005

AbstractNeuroblastoma is the most common extracranial solid tumor in children. Abnormal secretion of catechol-amines in tissues and body fluids allows for the differential diagnosis of neuroblastoma from other neoplasms and its distinction from non-neoplastic inflammatory diseases. This is achieved by assaying homovanillic acid and 4-hydroxy-3-methoxymandelic acid, the catabolites of catecholamine metabolism. In the course of an evaluation of children with suspected neuroblastoma, homovanillic acid and 4-hydroxy-3-methoxymandelic acid were analyzed in urine samples by capillary gas chromatography with flame ionization detection after extraction and derivatization of these compounds as trimethylsilyl derivatives. In three urine samples a significant increase in biogenic amines was observed, but these results were not confirmed by thin-layer chromatography. Patient history revealed that these children had been treated with ibuprofen, an analgesic and anti-inflammatory drug. To verify how ibuprofen or its metabolites may have interfered with capillary gas chromatography with flame ionization detection, we analyzed the same samples by capillary gas chromatography-mass spectrometry. In urine samples from patients on the drug, the presence of a peak identified as the trimethylsilyl ester of hydroxyibuprofen, which had the same retention time as 4-hydroxy-3-methoxymandelic acid, was found to interfere with the capillary gas chromatography with flame ionization detection analysis of the metabolite. This interference must be taken into account during the laboratory diagnosis of neuroblastoma.
Selective measurement of HCHO in urine using direct liquid-phase fluorimetric analysis
Authors: Chandler Short Luke , Benter Thorsten 
Published: February 2005

AbstractQuantification of formaldehyde (HCHO) in urine was recently shown to be a promising tool in the investigation of cancer, particularly bladder cancer. Development of a low-maintenance, inexpensive and rapid analyzer for HCHO in urine would greatly facilitate future research and the potential diagnosis of bladder cancer. We examine here the application of an off-the-shelf system, originally designed for gas-phase atmospheric monitoring of HCHO, for the quantification of HCHO in urine. Under strict dietary protocols, e.g., avoidance of foods rich in free or chemically bound HCHO, an increase in HCHO in urine is an indirect indicator of cancer in the urogenital system. The concentration of HCHO in urine samples from an individual over a several-month period was determined, with a range from 39 to 1400μM and a mean of 600μM. The limit of detection for the present method was 0.1μM. The proposed technique provides a direct, low-cost and greatly simplified analytical method for the quantification of HCHO in urine compared to other available techniques.
A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
Authors: Kufner Guenther , Schlegel Helmut , Jäger Reinhard 
Published: February 2005

AbstractAn increased concentration of erythrocyte protoporphyrin-IX in whole blood or erythrocytes is a valuable diagnostic indicator for acquired porphyrias, e.g., iron deficiency anemia and lead poisoning, and for inherited porphyrias. We developed a spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX. In this method, exhaustive release of erythrocyte porphyrins is achieved using hydroquinone and formic acid. The clean-up procedure for 50μL of whole blood or erythrocytes covers three steps of liquid/liquid solvent partition: two partitions using diethyl and diisopropyl ether and HCl 2.5mol/L, and one buffered step using ammonium formate. Determinations of erythrocyte protoporphyrin-IX are possible by: (a) absorption using three wavelengths, Rimington's constant and a millimolar absorptivity coefficient mɛ
Simultaneous analysis of MDR1 C3435T, G2677T/A, and C1236T genotypes by multiplexed mutagenically separated PCR
Authors: Sunder-Plassmann Raute , Rieger Sandra , Endler Georg , Brunner Martin , Müller Markus , Mannhalter Christine 
Published: February 2005

AbstractP-Glycoprotein (PGP) encoded by the multi-drug-resistance 1 (
Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor
Authors: Noronha José F. A. , Grotto Helena Z. W. 
Published: February 2005

AbstractNew parameters correlated with the hemoglobin content in reticulocytes (RET-Y) and in red blood cells (RBC-Y) have been suggested as helpful in diagnosing iron deficiency anemia. We have studied RET-Y and RBC-Y indices in two groups of patients with microcytosis to verify if these parameters could be used to differentiate iron deficiency anemia from β-thalassemia minor. Blood samples from 33 iron-deficient patients, 25 β-thalassemic minor patients and 50 normal individuals were analyzed on a Sysmex XE-2100 instrument. A significant difference was observed in reticulocyte counting and immature reticulocyte fraction between iron deficiency anemia and β-thalassemia minor groups, but not in RBC-X and RET-Y parameters. Reticulocyte counting was higher in β-thalassemia minor and the immature reticulocyte fraction was higher in severe iron deficiency anemia. The ratio RET-Y/mean cell volume was tested and was significantly different when β-thalassemia minor was compared with mild and severe iron deficiency anemia, and showed better performance than the Mentzer ratio and the Green and King function. A great overlap of RET-Y and RBC-Y individual values was observed in both groups of microcytic anemias; we conclude that these new indices may be used with caution as indicative of iron deficiency, mainly in populations where β-thalassemia minor is frequent.
Chitotriosidase activity in colostrum from African and Caucasian women
Authors: Musumeci Maria , Malaguarnera Lucia , Simpore Jacques , Barone Rita , Whalen Michael , Musumeci Salvatore 
Published: February 2005

AbstractChitotriosidase (ChT), a protein produced by activated macrophages, belongs to the chitinases, a group of enzymes able to hydrolyze chitin, a structural component of fungi and nematodes. A codominant inherited deficiency in ChT activity is frequently reported in plasma of Caucasian subjects, whereas in the African population this deficiency is rare. This study compares ChT activity in colostrum of 53 African women and 50 Caucasian women. Samples were collected at 24–48 and 72h after delivery. We found elevated ChT in colostrum of African women on the first day after delivery (1230±662nmol/mL/h) which decreased to 275±235nmol/mL/h on the third day. The ChT activity on the first day after delivery in the colostrum of Caucasian women, however, was significantly lower (293±74nmol/mL/h) and decreased to 25±20 and 22±19nmol/mL/h on the 2nd and 3rd day, respectively. The ChT activity in plasma of African women was also higher (101±80nmol/mL/h) than that of Caucasian women (46±16nmol/mL/h), but no correlation was found between plasma and colostrum ChT activity. The elevated ChT activity in colostrum of African women suggests the presence of activated macrophages in human milk, consistent with the genetic characteristics of the African population.
Controlled storage conditions prolong stability of biochemical components in whole blood
Authors: Stahl Marta , Brandslund Ivan 
Published: February 2005

AbstractBlood specimens from primary care centres are normally transported to central laboratories by mail. This necessitates centrifugation and separation, especially since the potassium ion concentration in whole blood changes during storage at ambient temperature. Thus, because of the growing awareness of and concern for pre-analytical contributions to the uncertainty of measurements, we investigated 27 components and their stability under controlled temperature conditions from 17 to 23°C. We found that storage of whole blood can be prolonged by up to 8–12h for all components examined, including potassium ions, when stored at 20±0.2°C. We conclude that this opens the possibility for establishing a pick-up service, by which whole blood specimens stored at 20–21°C can be collected at the doctor's office, making centrifugation, separation and mailing superfluous. In addition, the turn-around time from sample drawing to reporting the analytical result would be shortened. After investments in thermostatted boxes and logistics, the system could reduce costs for transporting blood samples from general practice centres to central laboratories.
Poor knowledge and faulty thinking regarding hemolysis and potassium elevation
Authors: Hawkins Robert C. 
Published: February 2005

AbstractA questionnaire to assess knowledge of the expected elevation in serum K measurement with different grades of hemolysis was administered to medical technologists working in biochemistry laboratories, hospital physicians and nurses. The questions involved different grades of hemolysis (mild, 1.0, moderate, 2.5 and severe, 5.0g/L) and different final K measurements (2.9, 4.0, 5.2 and 8.2mmol/L). Subjects estimated the K concentration in a non-hemolyzed sample for each scenario. Adjustment values (difference between final hemolyzed K concentration and subject's response) were calculated. For the 132 respondees, the mean correct score was 1.7/12. Mean adjustment values were: mild, 0.43mmol/L (K 2.9), 0.55 (4.0), 0.88 (5.2) and 1.53 (8.2); moderate, 0.85 (2.9), 0.92 (4.0), 1.33 (5.2) and 2.50 (8.2); and severe, 0.93 (2.9), 1.48 (4.0), 1.96 (5.2), 2.96 (8.2). Correct adjustments were: mild, 0.28; moderate, 0.70; and severe, 1.40mmol/L. Healthcare staff overestimated the effect of hemolysis on potassium measurement and used an incorrect proportional adjustment approach to the problem. Such poor knowledge and faulty thinking could lead to diagnostic delays or misdiagnoses. There is potential for such faulty thinking in all areas of laboratory medicine, and laboratories should review their educational responsibilities and reporting practices in light of this.
Delayed effects of short-term transdermal application of 7-oxo-dehydroepiandrosterone on its metabolites, some hormonal steroids and relevant proteohormones in healthy male volunteers
Authors: Šulcová Jarmila , Hampl Richard , Hill Martin , Stárka Luboslav , Nováček Alois 
Published: February 2005

AbstractTwenty-one healthy male volunteers aged 20–70years were given transdermally 25mg of 7-oxo-dehydroepiandrosterone daily in the form of an emulgel for 8 consecutive days. Morning blood was collected as follows: before application, and after the first, fourth and eighth doses (days 0, 2, 5 and 9), and then at different time intervals after termination of the treatment (days 16, 23, 37, 51, 72 and 100). Cortisol, testosterone, epitestosterone, estradiol, dehydroepiandrosterone and its sulfate, 7α- and 7β-hydroxy-dehydroepiandrosterone, luteinizing hormone, follicle-stimulating hormone and sex hormone-binding globulin were measured in blood sera. In the course of treatment 7β-hydroxy-dehydroepiandrosterone was significantly increased; testosterone and gonadotropins were lowered, but only after the first dose. All other significant changes were observed duringthe period after termination of the application:7β-hydroxy-dehydroepiandrosterone remained increased for 28days, 7α-hydroxy-dehydroepiandrosterone, testosterone, estradiol and sex hormone-binding globulin were decreased as late as day 63 and 91, respectively. On the other hand, epitestosterone was significantly increased between days 23 and 100. The levels of all other parameters studied were not significantly changed. The study points to an immediate as well as delayed effect of the short-term transdermal application of 7-oxo-dehydroepiandrosterone on relevant hormonal parameters.