bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative effects of phytohormones in the phenolic acids production in Salvia verticillata L. under multi-walled carbon nanotubes and methyl jasmonate elicitation
Authors: Rahmani Nosrat , Radjabin Tayebeh 
Published: December 2023

Abstract
Salvia verticillata L. is a well-known herbal medicine rich in rosmarinic acid (RA) and with therapeutic values. Some physiological and biochemical responses of S. verticillata to methyl jasmonate (MJ) and multi-walled carbon nanotubes (MWCNTs) were investigated in this work. It has been supposed that the stimulants could change the level of phenolic compounds, especially RA, and activate their signaling pathways in S. verticillata. The leaves were sprayed with aqueous solutions containing 100 mg L-1 MWCNTs and 100 μM MJ and then harvested from 0 to 96 hours after treatment. Obtained data revealed significant variations in the levels of some phytohormones, as well as the content of H2O2 and the amount of lipid peroxidation, during the initial hours of harvesting. The content of phenolic acids positively correlated with the transcription levels of key enzymes involved in their biosynthesis, especially rosmarinic acid synthase. The content of RA increased more than 2.7- and 11.5-fold following exposure to MJ and MWCNTs, respectively. The patterns of changes in phytohormone levels in the treated leaves with MWCNTs were different from those in leaves elicited with MJ. It is reasonable to conclude that putative linkages between changes in some phytohormone pools, notably jasmonic acid and salicylic acid, and applied elicitors lead to the accumulation of phenolic acids in the leaves of S. verticillata. Overall, the present findings help us to improve our understanding of the signal transduction pathways of the applied stimulants that led to the enhancement of phenolic acids in S. verticillata.
Association of low hemoglobin at birth and neurodevelopmental outcomes in preterm neonates ≤28 weeks’ gestation: A retrospective cohort study
Authors: Mohamed Adel , Elkhouli Mohamed , Raghuram Kamini , Elhanafy Thanna , Asztalos Elizabeth , Banihani Rudaina , Shah Prakeshkumar 
Published: December 2023

Abstract
Objective To compare neurodevelopmental outcomes at 18–24 months corrected age (CA) for preterm infants who had hemoglobin levels < 120 gm/l versus those with hemoglobin level ≥ 120 gm/l at birth.Methods We included infants of ≤ 28 weeks gestational age (GA) born between January 2009 and June 2018. The primary outcome was neurodevelopmental impairment (NDI) at 18–24 months. Multivariable logistic regression was applied to determine the association.Results Of the 2351 eligible neonates, 351 (14.9%) had hemoglobin levels < 120 gm/L at birth. Of the 2113 surviving infants, 1534 (72.5%) underwent developmental follow-up at 18 to 24 months CA. There was no statistically significant difference in ND outcomes between the two groups. The composite outcome of death or NDI was significantly higher in the low hemoglobin group.Conclusion In preterm infants ≤ 28 weeks GA, initial hemoglobin < 120 gm/L at birth was not associated with neurodevelopmental impairment at 18–24 months CA among survivors.
A Case of Eosinophilic Granulomatosis with Polyangiitis Combined with Pulmonary Tuberculosis: A Case Report
Authors: Lai Yuting , Xiao Shan , Yan Shen 
Published: December 2023

Abstract
Background Eosinophilic Granulomatosis with Polyangiitis (EGPA) is a rare autoimmune disease that can affect multiple organ systems. The standard treatment mainly relies on glucocorticoids and immunosuppressive agents. In our study, we present an EGPA patient who had pulmonary tuberculous mycobacteria infection, such casesare rarely reported.
Case presentations A 71-year-old male patient was diagnosed with EGPA (systemic type) and pulmonary tuberculosis simultaneously. A 5-factor score indicated that the patient required glucocorticoids combined with immunosuppressive agents for induction therapy, however, the use of immunosuppressive agents would significantly inhibit anti-tuberculosis treatment. Nowadays, treating active autoimmune disease in patients with infections remains a clinical challenge.
Considering the patient did not show life-threatening or severe organ involvement and reduced the effect of anti-tuberculosis immunity, we used glucocorticoids alone. Finally, the patient had no adverse events, the eosinophil counts were markedly decreased and symptoms of EGPA were relieved.
Conclusions The patient of EGPA combined with pulmonary tuberculosis successfully treated with glucocorticoids alone may provide significant support in selecting the appropriate treatments for similar cases in the future.
Postharvest Application of 1-Methylcyclopropene (1-MCP) Smart Fresh on quality of banana cv. Grand Naine
Authors: Jayavalli R 
Published: December 2023

Abstract
Maintaining the postharvest quality of fresh bananas is a major trading challenge of the industry. 1- Methylcyclopropene (1-MCP) can prolong the postharvest quality of banana fruits by delaying the expression of ripening attributes and senescence through overtaking the binding of ethylene to its receptors thereby inhibiting ethylene signal transduction and downstream action. In the present study the treatments imposed were T1- Application of 1-Methylcyclopropene (1-MCP) 3.3% at 70mg/m3 (1ppm 1-MCP) in cold storage, T2- immediately after Application of 1-Methylcyclopropene (1-MCP) under room temperature, T3- Untreated control under cold storage, T4- Untreated control under room temperature or ambient temperature at 28 ± 20 C. (1-MCP) was applied for 14 hr (overnight) in an airtight container or in the 4 m3 tent with a fan inside to favour 1- MCP quick distribution. Observations were recorded for 5, 15 21 and 28 days under cold storage conditions of treated and untreated control fruits and without cold storage of treated and untreated control. The postharvest quality of ‘Grand Naine’ banana fruits such as peel yellowing, sensory firmness, TSS, visual quality, weight loss, shelf life and disease incidence under (1-MCP) for 14 hr (overnight) in an airtight container with a fan inside to favour 1- MCP quick distribution had prolonged the storage and shelf life of fruits. Moreover, weight loss of 1-MCP treated fruits was slower than control as evident on day 5 of storage onwards. Good visual quality of fruits treated with 1-MCP through gas exposure was maintained for 28 days of storage and control for 10 days only. However, disease incidence of the fruits among all 1-MCP treatment methods and control was comparable under similar storage condition and 1-MCP concentration.
Differences in Mental Health Characteristics of Athletes in Japanese Outpatient Clinics Pre- and Post-covid-19: a Cross-sectional Observational Study
Authors: Yamaguchi Tatsuya , Kitahara Akari , Sato Kazuki , Mimura Masaru 
Published: December 2023

Abstract
Background
Mental health disorders and symptoms in athletes have received increasing attention in recent decades, with the COVID-19 pandemic intensifying the psychological distress of athletes. While there have been numerous studies on athletes’ visits to mental health clinics in other countries, no study has been conducted on this topic in Japan. Therefore, this study investigated the differences in characteristics between athletes who visited mental health outpatient clinics before and after the COVID-19 pandemic.
Methods
A cross-sectional observational survey was conducted using data used from Keio University General Hospital and three affiliated mental clinics. Our sample comprised 88 athletes who had visited various healthcare facilities between 2018 and 2022. The independent variable was the timing of their outpatient mental health clinic visits—either pre- or post-COVID-19. The primary outcome was the F classification based on the International Statistical Classification of Diseases and Related Health Problems 10th Revision. We collected data on clinical psychiatric diagnoses, age, sex, referral pathway, and sports-related details like the type, level of contact, whether individual or team sport, involvement duration, and athletic competition level.
Results
Overall, 86 athletes (37 before COVID-19 and 49 after COVID-19) were included in the study. No significant differences were found between the pre- and post-COVID-19 groups overall. However, subgroup analyses revealed a significant increase in anxiety-related disorders among female athletes and an increase in the participation of male athletes in individual sports in the post-COVID-19 group compared to the pre-COVID-19 group.
Conclusion
The increased anxiety among female athletes and the increased participation in individual sports among male athletes suggest that these groups should be a high-priority target for early intervention and prevention strategies. This study contributes to our understanding of how COVID-19 has affected the mental health of athletes seeking medical treatment in Japan, and it highlights which segments of the Japanese athlete population may be more vulnerable to mental health issues in the post-COVID-19 era. This study also provides valuable insights for practitioners on how to target specific segments of the athlete population for the implementation of interventions aimed at mitigating the development of mental health issues after COVID-19.
Roles of estrogen receptors during sexual reversal in Pelodiscus sinensis
Authors: Chen Guobin , Zhou Tong , Cao Jizeng , Li Xiang , Zhu Chengjun , Wang Long , Zou Guiwei , Liang Hongwei 
Published: December 2023

Abstract

The Chinese soft-shelled turtle, Pelodiscus sinensis, exhibits distinct sexual dimorphism, with the males growing faster and larger than the females. During breeding, all-male offspring can be obtained using 17β-estradiol (E2). However, the molecular mechanisms underlying E2-induced sexual reversal have not yet been elucidated. Previous studies have investigated the molecular sequence and expression characteristics of estrogen receptors (ERs). In this study, primary liver cells and embryos of P. sinensis were treated with ER agonists or inhibitors. Cell incubation experiments revealed that nuclear ERs (nERs) were the main pathway for the transmission of estrogen signals that was EB upregulated the expression of Rspo1, whereas AS downregulated. The expression of Dmrt1 was enhanced after AS + G-1 treatment, indicating that the regulation of male genes may not be through a single estrogen receptor, but a combination of ERs. As to embryos, only the ERα agonist (EB) remarkably promoted the expression levels of Rspo1, Wnt4, and β-catenin, whereas the ERα inhibitor (AS) had a suppressive effect. Additionally, Dmrt1, Amh, and Sox9 expression levels were downregulated after ERβ inhibitor (PHTPP) treatment. GPER agonist G-1 has a significant promotion effect on Rspo1, Wnt4, and β-catenin, while the inhibitor G-15 has no effect on male-related genes. Overall, these results suggest that ERs play different roles during sexual reversal in P. sinensis and ERa may be the main carrier of estrogen-induced sexual reversal in P. sinensis. Further studies need to be performed to analyze the mechanism of ER action.
WITHDRAWN: New Breakthrough in Triple-Negative Breast Cancer Treatment: A Study on the Clinical Efficacy and Safety of the Combination of Carrilizumab and Apatinib in Dual-Targeted Neoadjuvant Therapy
Authors: YIN Yulai , ZHANG Xiaoyu 
Published: December 2023

Abstract

Objective To investigate the clinical efficacy and safety of dual-targeted neoadjuvant therapy combining carrilizumab with Apatinib in patients with triple-negative breast cancer.
Methods This study collected data from 76 patients with triple-negative breast cancer, confirmed as PD-L1 positive (CPS ≥ 1%) via immunohistochemistry and core needle biopsy, treated at Cangzhou Central Hospital from March 2021 to March 2022. Patients were randomly divided into a control group (n=38) and an observation group (n=38). Based on the standard AT chemotherapy regimen, the control group received treatment with the PD-1 inhibitor carrilizumab, while the observation group was treated with a combination of carrilizumab and the anti-angiogenic drug Apatinib. After completing neoadjuvant therapy, the objective response rate, major pathological response rate, pathological complete response rate, breast conservation rate, CD4+ T lymphocyte subpopulation levels, vascular endothelial growth factor levels, and Ki-67 and PD-L1 expression were compared between the two groups.
Results After four cycles of neoadjuvant therapy, the observation group showed a superior major pathological response rate, pathological complete response rate, and elevated CD4+ T lymphocyte levels compared to the control group (P < 0.05). There was a significant decrease in the proportion of high Ki-67 expression in the observation group (P < 0.05), and the levels of vascular endothelial growth factor were lower than in the control group (P < 0.05). Although the breast conservation rate was relatively higher in the observation group, the difference was not significant (P > 0.05). The incidence of adverse events was similar in both groups, except for a higher rate of hand-foot syndrome in the observation group (P > 0.05).
Conclusion The dual-targeted neoadjuvant therapy for triple-negative breast cancer showed considerable clinical efficacy in reducing tumor burden and was acceptably safe.
First aid Knowledge and Bystander Willingness to Rescue in Jilin, China: The Mediating Roles of Attitude towards First Aid
Authors: Guo Xinrong , Kassa Getachew Mullu , Ye Lin , Li Junxin , Kang Sijia , Zheng Huiling , Wang Yiwen , Liu Wandi 
Published: December 2023

Abstract
Background
Previous research has been conducted on bystander first aid knowledge, attitudes, and willingness. However, surveys of their willingness to provide first aid as bystanders have shown conflicting results. Jilin Province, China, as an economically underdeveloped region, has a low popularization rate of first aid knowledge, and there is a lack of analysis of the mediating effects on first aid knowledge, attitude, and willingness to rescue. Therefore, this study will investigate the first aid knowledge, attitude, and willingness level of residents in Jilin Province as bystanders, and explore the influencing factors of first aid willingness.
Methods
A cross-section study design using a convenience sampling method was used to recruit participants from 16 communities in 8 regions in Jilin Province, China, from May to August 2021. The study included a total of 1994 participants in the analysis. Logistic regression and mediation analysis were conducted to examine the factors associated with and influence of first-aid attitude as the mediating variable to establish a mediation model of the first-aid mechanism. The strength of the association between the independent variables with the willingness to rescue was analyzed using crude odds ratios (COR) of binary logistic regression and adjusted odds ratios (AOR) of multiple logistic regression. Statistical significance was declared when the p-value was < 0.05.
Results
Only 366 (18.4%) had a high level of willingness. The results of multiple logistic analyses showed that gender, occupation, education level, and income level were significantly associated with willingness to rescue. The intermediary analysis found that first-aid attitude was the mediator between first-aid knowledge and willingness, and the mediating effect accounted for 59.70% of the total effect.
Conclusions
The willingness of residents in Changchun to receive first aid is at a relatively low level, which is influenced by gender, occupation, education level, and economic level. Relevant units and departments should increase training efforts and develop corresponding training management strategies based on the characteristics of the population to promote the popularization of public first aid knowledge and improve the success rate of first aid.
Prediction of Greenhouse Gases Solubility in Eutectic Solvents using COSMO-RS
Authors: Pelaquim Fernanda Paludetto , Vilas-Boas Sérgio M. , Nascimento Débora Costa , Carvalho Pedro Jorge , Neto Antonio Marinho Barbosa , Costa Mariana Conceição 
Published: December 2023

Abstract
Over the past few years, eutectic solvents (ESs) have been drawing the scientific community's attention because they are usually more environmentally friendly than traditional organic solvents. One of the applications of ESs is in the gas capture field, where they are considered promising absorbers to replace amine- (MEA, DEA, or MDEA processes), methanol- (Retinol process), dimethyl ethers of polyethylene glycol- (Selexol process), N-methyl-2-pyrrolidone- (Purisol process), propylene carbonate- (Fluor solvent process), or morpholine-based (Morphysorb process) solvents on CO2 capture of atmosphere. Although several studies have reported experimental gas solubility data in ESs, especially for CO2, only a small number of the existing options are covered. In fact, resorting to experimental methods to obtain the solubility data seems unfeasible considering the vast number of possible eutectic mixtures. Therewith, theoretical predictions of gas solubility in ESs are valuable for the fast pre-screening of prospective solvents. In this work, the ability of the COSMO-RS thermodynamic model to represent solubility data of CO2, CH4, and H2S in 17 Choline Chloride-based (ChCl) ESs was evaluated. The experimental data were collected from the literature at different molar ratios, at 298.15 K or 313.15 K, and in the pressure range from 1 to 125 bar. COSMO-RS offers a qualitative description of these gases' solubility, which was expected due to the model's fully predictive character.
Toripalimab combined with albumin-paclitaxel plus gemcitabine as first-line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma: an open-label, phase Ib/II study and investigation of potential biomarkers
Authors: Cao Dan , Cheng Ke , Zhao Gang , Lv Wanrui , Li Xiaoying , Chang Chen , Yang Heqi , Li Ruizhen , Zhang Yi , Xiong Junjie , Huang Zixing , Shao Weikang , You Xin , Guo Wenhao , He Du , Ling Wenwu , Wang Rui , Zhao Chengjian , Tian bole 
Published: December 2023

Abstract
Advanced pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis. Immunotherapy alone offers limited efficacy, but its combination with chemotherapy could offer synergist anti-tumor effects. This phase Ib/II study evaluated the safety and efficacy of combining toripalimab with the albumin-paclitaxel plus gemcitabine (AG) regimen as first-line treatment for locally advanced or metastatic PDAC. The primary endpoints were safety and overall survival (OS). The secondary outcomes were objective response rate (ORR), disease control rate (DCR), and progression-free survival (PFS). Immune-related biomarkers including programmed death-ligand 1 (PD-L1) expression, genetic status, cytokine levels, and immune microenvironment were investigated. Among the 72 patients enrolled, the median OS was 8.8 months, and the 12-month OS rate was 33.3%. No serious treatment-related adverse events, grade 4 immune-related adverse events, or treatment-related deaths were reported. The combination therapy led to a median PFS of 5.3 months, ORR of 33.3%, and DCR of 90.3%. Non-liver metastasis status predicted a better PFS, and the number of lesion sites and baseline CA199 level were independently associated with OS. One patient (1.4%) with mismatch repair deficiency achieved pathologic complete response and prolonged OS > 19 months. Higher PD-L1 expression was associated with a better ORR than lower expression (56.3% vs 25%), and a higher CD3 + cell density, decreased CD68 + cell infiltration, and lower baseline serum interleukin-8 level were associated with better treatment response. Overall, AG plus toripalimab exhibited a certain level of safety and probable clinical efficacy for locally advanced or metastatic PDAC, especially in some potentially advantaged subgroups.
Artificial Intelligent Tools: Evidence-Mapping on the Perceived Positive Effects on Patient-Care
Authors: Botha Nkosi Nkosi , Ansah Edward Wilson , Segbedzi Cynthia Esinam , Dumahasi Victor Kwasi , Maneen Samuel , Kodom Ruby Victoria , Tsedze Ivy Selorm , Akoto Lucy Adjanor , Atsu Fortune Selasi 
Published: December 2023

Abstract
The global healthcare system is fraught with serious challenges including scarcity of critical healthcare professionals, changes in disease patterns, pandemics, access and equity issues among others. Considering that there is no quick fix to the myriad of healthcare challenges, World Health Organisation proposed a full integration of artificial intelligent (AI) tools into patient-care to stimulate efficiency and guarantee quality in patient-management. Therefore, this review maps evidence on the perceived positive effects of AI tools on patient-care. The review considered time expand between January 1, 2010 and October 31, 2023. Consistent with the protocol by Tricco et al., a comprehensive literature search was executed fromNature, PubMed, Scopus, ScienceDirect, Dimensions, Ebsco Host, ProQuest, JStore, Semantic Scholar, Taylor & Francis, Emeralds, WHO, and Google Scholar. Upholding the inclusion and exclusion standards, 14 peer reviewed articles were included in this review. We report the use of that AI tools could significantly improve accuracy of clinical diagnosis and guarantee better health-outcomes of patients. AI tools also have the ability to mitigate, if not eliminate, most of the factors that currently predict poor patient outcomes. Furthermore, AI tools are far more efficient in generating robust and accurate data in real-time, and could help ease and accelerate the workflow. If properly integrated into the healthcare system, AI could help accelerate the attainment of Sustainable Development Goals 3.4, 3.8, and 3.b. We propose that AI developers collaborate with public health practitioners and healthcare managers to develop AI applications that appreciate socio-cultural dimensions in patient-care.