bluuebunny/crossref_metadata_2025_split huggingface dataset:
Density of dispersal sources affects to what extent restored habitat is used: a case study on a red-listed wood-dependent beetle
Authors: Djupström Line Boberg , Johansson Victor , Lindman Ly , Schroeder Martin , Weslien Jan , Ranius Thomas 
Published: October 2023

Abstract
When restoring habitats, an important question is whether the spatial distribution of habitat affects its contribution to biodiversity conservation. In Sweden, high-cut stumps are routinely created at forestry operations. By counting the number of exit holes of a red-listed beetle, Peltis grossa, we assessed occurrence, colonisations and extinctions per high-cut stump and beetle density per clear-cut. We found a threshold, at which the form of the relationship between density of the beetle and density of high-cut stumps per clear-cut changes abruptly. The beetle density was considerably higher where the density of high-cut stumps exceeded 4.5 per hectare. Such thresholds can be explained by colonisation-extinction processes. Observed colonisation-extinction dynamics were consistent with metapopulation theory. For instance, there was a positive relationship between colonisation rate and a connectivity measure that considered beetle abundance and distance for each high-cut stump in the surrounding area. However, the relationship disappeared when using a connectivity measure solely based on the distance of the high-cut stumps. The observed threshold implies that P. grossa benefits from aggregating the same total number of created high-cut stumps into fewer clear-cuts. This is because the total area with a density of high-cut stumps exceeding the threshold increases, and this expands the number and size of dispersal sources. Therefore, P. grossa and other species that reveal thresholds in their distribution patterns, are favoured when conservation measures are more spatially aggregated than what is resulting from current Swedish policy.
Sexual Dimorphism during Integrative Endocrine and Immune Responses to Ionizing Radiation in Mice
Authors: Burke Marissa , Wong Kelly , Talyansky Yuli , Mhatre Siddhita D. , Mitchell Carol , Juran Cassandra M. , Olson Makaila , Iyer Janani , Puukila Stephanie , Tahimic Candice G.T. , Christenson Lane K. , Lowe Moniece , Rubinstein Linda , Shirazi-Fard Yasaman , Sowa Marianne B. , Alwood Joshua S. , Ronca April E. , Paul Amber M. 
Published: October 2023

Abstract
Exposure to cosmic ionizing radiation is an innate risk of the spaceflight environment that can cause DNA damage and altered cellular function. In astronauts, longitudinal monitoring of physiological systems and interactions between these systems are important to consider for mitigation strategies. In addition, assessments of sex-specific biological responses in the unique environment of spaceflight are vital to support future exploration missions that include both females and males. Here we assessed sex-specific, multi-system immune and endocrine responses to simulated cosmic radiation. For this, 24-week-old, male and female C57Bl/6J mice were exposed to simplified five-ion, space-relevant galactic cosmic ray (GCRsim) radiation at 15 and 50 cGy, to simulate predicted radiation exposures that would be experienced during lunar and Martian missions, respectively. Blood and adrenal tissues were collected at 3- and 14-days post-irradiation for analysis of immune and endocrine biosignatures and pathways. Sexually dimorphic adrenal gland weights and morphology, differential total RNA expression with corresponding gene ontology, and unique immune phenotypes were altered by GCRsim. In brief, this study offers new insights into sexually dimorphic immune and endocrine kinetics following simulated cosmic radiation exposure and highlights the necessity for personalized translational approaches for astronauts during exploration missions.
“I still don’t know if I am doing the right thing”: A qualitative study of experiencing cauda equina syndrome and its aftercare in the UK.
Authors: Srikandara Nisaharan , Clark Simon , Wilby Martin , Marson Tony , Noble Adam 
Published: October 2023

Abstract
STUDY DESIGN
Qualitative, semi structured interviews.
OBJECTIVES
Cauda Equina Syndrome (CES) is a neurological emergency that can cause permanent disability to the lower limbs, including pain, weakness, and bladder, bowel and sexual dysfunction. There is little evidence on the lived experience of patients with different severities of CES. This study sought to address this.
SETTING
The interviews were conducted with persons who had experienced CES and been operated on for this condition in the UK.
METHODS
A sampling frame was used on a pre-existing database to select a maximum variation sample. Interviews were audio recorded and transcribed for thematic analysis supported by NVivo.
RESULTS
Data saturation was achieved with 22 patients (12 female, 10 male) of whom 10 had CES-incomplete and 12 had CES-complete. Average age was 46 years and time since the operation was 62 months. Most interviews took place at the patients’ home or workplace. Data analysis identified 4 main data themes: 1) Varying priorities of physical health; 2) A fragmented healthcare service; 3) The process of adjustment; and 4) Anticipatory anxiety and diminished sense of self-worth.
CONCLUSION
Currently, CES is managed as an acute condition in hospital. The data themes present CES can be a chronic condition, which requires holistic support to address long-term outcomes. This highlights the importance of using the Cauda Equina Syndrome Core Outcome Set (CESCOS) in CES research studies to record these outcomes.
Fungal toxin fusicoccin enhances plant growth by upregulating 14-3-3 interaction with plasma membrane H+-ATPase
Authors: Ohkanda Junko , Kiriyama Hironaru , Kinoshita Satoru , Hayashi Yuki , Kasuga Shigemitsu , Kinoshita Toshinori , Irieda Hiroki 
Published: October 2023

Abstract

Fusicoccin-A (FC-A) is a diterpene glucoside produced by a pathogenic fungus. FC-A is phytotoxic and induces stomatal opening and leaf wilting, eventually leading to plant death. Here, we report that FC-A enhances plant growth by stabilizing the protein-protein interaction between plasma membrane (PM) H+-ATPase and 14-3-3 in guard cells, promoting stomatal opening and thus photosynthesis. Long-term treatment of Arabidopsis plants with FC-A resulted in ~ 30% growth enhancement. Structurally similar fusicoccin-J (FC-J) showed a similar degree of growth-promotion activity as FC-A, whereas the more hydrophilic fusicoccin-H (FC-H) exhibited no effect on plant growth, indicating that the enhancement of plant growth observed with FC-A and FC-J involves upregulation of the protein-protein interaction between PM H+-ATPase and 14-3-3 in guard cells, which promotes stomatal opening and photosynthesis.
Implicitly Activating Mindfulness: Does Trait Self-Control Moderate Its Effect on Aggressive Behaviour?
Authors: Thériault Rémi , Dandeneau Stéphane 
Published: October 2023

Abstract
Objectives.
Previous research shows that a novel experimental paradigm consisting of implicitly activating (“priming”) concepts associated with mindfulness through a scrambled sentence task yields positive social effects on cognition and affect. Yet, its effects on social behaviour warrant further investigation. As several studies link mindfulness to lower aggression, the latter represents a promising candidate to investigate within the current paradigm. Furthermore, research has demonstrated that personality traits—such as trait mindfulness—moderate the effect of the mindfulness prime, highlighting the importance of identifying potential moderators.
Methods.
In a first hypothesis-generating (exploratory, non-preregistered) study, we investigated which of several personality variables most meaningfully related to the priming mindfulness procedure.
Results.
Self-control emerged as the only meaningful moderator. Accordingly, we specifically tested the interaction between self-control and the mindfulness priming procedure in two additional hypothesis-testing (confirmatory, preregistered) studies using larger samples. The results from the first study did not replicate: the critical interaction of interest was not significant in both studies.
Conclusions.
We discuss the implications of these conflicting results and highlight the importance of preregistered replication studies in mindfulness research.
Preregistration.
The last two studies were preregistered on the Open Science Framework (https://osf.io/582wx/?view_only=147b4bcee2de46f8aea34258d5e08122 and https://osf.io/w46r9/?view_only=b71b93ac96814967af034fb1fb6fda7f).
First Swedish case of fatal equine parasitic encephalitis by Halicephalobus gingivalis
Authors: Olofsson-Sannö Karin Maria , de Velde Norbert van , Peletto Simone , Iulini Barbara , Pratley Laura , Modabberzadeh Behzad , Małek Emilian , Grandi Giulio 
Published: October 2023

Abstract

Background:Halicephalobus gingivalisis a nematode with zoonotic potential which can cause fatal opportunistic infections in a wide variety of mammals. The parasite has never been diagnosed in Sweden, in any species, prior to the presented case.Case presentation:An imported 21-year-old Icelandic mare developed severe neurological symptoms. The horse was eventually euthanized and submitted for post-mortem examination where severe lesions in the kidneys was noted. Samples for histological examination were submitted, which revealed the presence ofH. gingivalisin both renal and brain tissue. Phylogenetic analysis of the parasite determined it to belong to Linage 1.Conclusions:With the occurrence ofH. gingivalisin Sweden, the disease should be added to the list of differential diagnoses in cases with acute onset of neurological disease in both horses and mammals including humans.
Predictors of postoperative recurrence of pheochromocytoma: A monocentric study
Authors: Li Zhuoran , Lai Dong , Jia Yuqi , Luo Jin , Ma Xin , Zhang Xu , Tang Lu , Li Xintao , Wang Baojun 
Published: October 2023

Abstract
Background
To discuss the risk factors affecting the recurrence of pheochromocytoma after surgery.
Methods
We retrospectively reviewed patients who were hospitalized and underwent surgery for PCC between January 2012 and December 2020 at Chinese PLA General Hospital. Inclusion criteria were pathological diagnosis of PCC and availability of follow up.
Results
In total, 451 patients met the inclusion criteria. The average age was 45.89 years, and the median tumor diameter was 5.75 cm. The mean recurrence time was 34.24 months. Of the 451 patients receiving surgery, there were 35 recurrent cases (7.85%). The univariate test showed that age, hypertension, history of PCC recurrence, Ki-67 index ≥ 5, bilateral tumor, duration of phenazopyridine administration, DBP at admission, open operation, intraoperative HR minimum, intraoperative times of HR over 120, times of instability, and intraoperative bleeding were associated with recurrence after radical surgery. Multivariate COX regression analysis of age (HR(hazard ratio) 0.95), hypertension (HR 7.14), history of PCC recurrence (HR 69.35), family history of hypertension (HR 16.30), bilateral tumor (HR 7.38), tumor size (HR 1.05), times of instability (HR 114.91) and length of instability in minutes (HR 1.12) were the independent influences on recurrence after pheochromocytoma resection.
Conclusions
Age, hypertension, history of PCC recurrence, family history of hypertension, bilateral tumor, tumor size, intraoperative times of instability, and intraoperative instability minutes were independent influences on recurrence after pheochromocytoma resection.
Targeted Delivery of Polyclustered-SPION Labeled Mesenchymal Stem Cells in Ototoxic Hearing Loss Mouse Model
Authors: Ahn Yeji , Choi Seonmin , Ho Tien Son , Choi Jin Sil , Ha Sun Mok , Batsaikhan Temuulen , Choi Seung Ho , Key Jaehong , Seo Young Joon 
Published: October 2023

Abstract
Background
Highly specialized cells with self-renewal and differentiation potentials are known to as stem cells. These cells can keep their stemness or develop into more specialized cells. Mesenchymal stem cell (MSC)-based cell treatment has caught researchers' interest due to its lack of ethical problems, simplicity of isolation, and abundance. The use of stem cell treatment in regenerative medicine has recently been a subject of great interest. In vitro and in vivo, MSCs have been shown to have anti-inflammatory and immunomodulatory properties. The ability of MSCs to decrease T-cell proliferation and reduce T-cell functioning is responsible for their potent immunosuppressive effects. They possess potential to home to injury site and stimulate indigenous cells to repair and rebuild it, despite their homing ability delivering the stem cell to the anatomically complex area such as inner is still remains as challenge. Studies on enhancing the delivery of stem cells has been emerging.
Method
The Superparamagnetic iron oxide nanoparticles (SPION) with an iron oxide-based nanoparticle cluster core covered with PLGA-Cy5.5 has been developed. To allow PCS nanoparticles to be transported into MSCs by endocytosis, we modified the nanoparticles for internalization. Nanoparticle labeled mesenchymal stem cells are administered in ototoxic mouse model with or without magnetic field.
Results
As an outcome, when compared to the control groups, the intratympanic administration with magnet group had the most cells in the brain, followed by the liver, cochlea, and kidney. The magnetic interaction between the produced PCS (poly clustered superparamagnetic iron oxide) nanoparticles and MSCs may increase stem cell delivery effectively. In a variety of experimental models examining cell responsiveness in vitro and in situ, the potential use of SPION to increase the spatial control of stem cells by the application of magnetic fields was examined. The application of the magnetic field has significantly increased the number of cells that migrate toward the area of magnet application in vitro. However, in in vivo settings the quantification of the effect of magnetic field was yet solved.
Conclusion
The visualization of delivered MSCs, as well as quantitative and qualitative analysis of the processes, will contribute in the formulation of an assessment system for a powerful delivery system in the inner ear, which will aid in the treatment of hearing loss
Genome-wide cross-disease analyses highlight causality and shared biological pathways of type 2 diabetes with gastrointestinal disorders
Authors: Adewuyi Emmanuel , Porter Tenielle , O’Brien Eleanor , Olaniru Oladapo , Verdile Giuseppe , Laws Simon 
Published: October 2023

Abstract

Observational studies suggest links between diabetes and gastrointestinal (GI) traits; however, the question of their underlying biological mechanisms and whether the observed relationship reflects shared genetic aetiology remains unresolved. Here, we comprehensively assess the genetic relationship between type 2 diabetes (T2D) and common GI disorders, leveraging several large-scale genome-wide association studies (GWAS) summary statistics. Our study demonstrates a significant positive global genetic correlation of T2D with peptic ulcer disease (PUD), irritable bowel syndrome (IBS), gastritis-duodenitis, gastroesophageal reflux disease (GERD), and diverticular disease, but not inflammatory bowel disease (IBD). Also, we identify several positive local genetic correlations (negative for T2D – IBD) contributing disproportionately to T2D's relationship with GI disorders. Multivariable Mendelian randomisation analyses suggest causal effects of genetic liability to T2D on PUD and gastritis-duodenitis, and a bidirectional causal association with GERD. Gene-based association analysis reveals gene-level genetic overlap between T2D and GI disorders, identifying several shared genes reaching genome-wide significance (Fisher’s combined P value [FCPgene] < 2.62 × 10− 6). Pathway-based functional enrichment analysis implicates leptin (T2D – IBD), thyroid, interferon, and notch signalling (T2D – IBS), QT interval anomaly and abnormal circulating calcium (T2D – PUD), viral, proinflammatory and (auto)immune-mediated mechanisms in T2D and GI disorders. Current findings provide evidence for a risk-increasing genetic overlap between T2D and GI disorders (except IBD), implicate shared genetically controlled biological pathways, highlight putative causality for certain T2D – GI disorders pairs, and identify important avenues for further exploration.
Achievements on Matrix Hilbert spaces and reproducing kernel matrix Hilbert spaces
Authors: Osgooei Elnaz 
Published: October 2023

Abstract
‎Hilbert space is a very powerfull mathematical tool that has proven to be incredibily useful in a wide range of applications‎. ‎Matrix Hilebrt space is a new frame work that has been presented recently to perform a matrix inner product space when data observation is proposed as matrices and it is needed to analyse large dataset with complex and multi-dimensional structure‎. ‎In this paper‎, ‎a new norm associated to sequences in matrix Hilbert spaces is defined and the relation between this new norm and the norm obtained by matrix inner product is investigated‎. ‎Also‎,‎it is discussed that although‎, ‎some results and concepts in Hilbert spaces can be extended to the matrix Hilbert spaces‎, ‎but counterexamples show that the results like Pythagorean theorem‎, ‎Parallelogram law‎, ‎Jordan-Von Neumann theorem are not hold in matrix Hilbert spaces‎. ‎At last the reproducing kernel Hilbert space (RKHS) is extended to reproducing kernel matrix Hilbert space (RKMHS) and by considering some appropriate conditions on a matrix Hilbert space‎, ‎an explicit structure of reproducing kernel is obtained and the RKMHS‎, ‎H(K)‎, ‎which is obtained by reproducing kernel $K=K_{1}+K_{2}$ is characterized‎.
Long-term evaluation of the safety of a rectal-prostate spacer, the ProSpace® balloon, in patients treated with radiotherapy for prostate cancer
Authors: Janoray G. , Bruguière E. , Mazurier J. , Dudouet P. , Guillotreau J. , Tollon C. , Labarthe P. , Seguin P. , Latorzeff I. 
Published: October 2023

Abstract

Background
Due to the close proximity of the prostate and rectum, rectal toxicity remains a major problem in patient treated by radiotherapy for prostate adenocarcinoma. One method of increasing the distance between the prostate and the rectum is to use a spacer implanted into the rectoprostatic space. This report describes the long-term outcomes obtained with a new ballon spacer.
Methods
Patients treated with curative radiotherapy for low- or intermediate-risk prostate adenocarcinoma, who underwent insertion of the ProSpace® (BioProtect Ltd, Tzur Yigal, Israel) rectal-prostate balloon spacer, were included. The main objective was to evaluate the dosimetric benefit of the spacer for OARs. The secondary objectives were to evaluate the feasibility and tolerability of ProSpace® balloon placement and to evaluate its long-term therapeutic efficacy and tolerance.
Results
Between October 2013 and March 2015, 16 patients were enrolled in the Pasteur Clinic, Toulouse, France. The median follow-up was 85.5 months. From top to bottom, the space created was a mean of 16.3 mm (range: 11–20.5 mm) at the base of the prostate, 12.1 mm (range: 4–16 mm) at the middle and 8.9 mm at the apex (range: 5–15 mm). On average, rectal volumes receiving a dose of 70 Gy, 60 Gy and 50 Gy were significantly lower after balloon implantation: -4.81 cc (1.5 vs. 6.3; p<0.0005), -8.08cc (6.4 vs. 14.5; p=0.002) and -9.06cc (16.7 vs. 25.7; p=0.003), respectively. There were significant differences in coverage after balloon implantation: Mean V95% (p<0.0005), mean Dmin (p=0.01) and mean V98% (p<0.001) were higher after balloon implantation. At 5 years, cumulative gastrointestinal toxicity was grade 1 in 6% (1/16 patients). No toxicity of grade 2 or higher was found. At 5 years, no urinary toxicity grade 3 or 4 toxicity was found. The QoL was not deteriorated.
Conclusions
The use of the ProSpace® balloon seems to be well accepted by patients, allowing a double dosimetric gain: a decrease in doses received by the rectum and an improvement in the coverage of the high-risk PTV. The long-term gastrointestinal toxicity remains low and QoL is preserved in all treated patients.
Fat1 suppresses the CSCs properties through CaMKII-mediated activation of the IFN pathway
Authors: Shang Zhengjun , Wang Jingjing , Chen yang , Sun Yunqing , Liu Hanzhe , Du Ruixue , Wang Xuewen , Shao Zhe , Liu Ke 
Published: October 2023

Abstract
FAT atypical cadherin 1 (Fat1), which encodes an atypical cadherin-coding protein, exhibits a high mutation rate and is commonly regarded as a tumor-suppressor gene in head and neck squamous cell carcinoma (HNSCC). Nonetheless, the potential regulatory mechanisms by which Fat1 influences the progression of HNSCC remain an unresolved enigma. In this context, we reported FAT1 was down-regulated in tumor tissues/cells when compared to the normal tissues/cells and correlated with the clinicopathological features and prognosis of HNSCC. Knockdown of FAT1 enhanced the CSCs properties and decreased the apoptosis rate of tumor cells. Mechanically, FAT1 knockdown increased the phosphorylation levels of Ca2+/calmodulin-dependent protein kinase II (CaMKII), subsequently resulting in a diminished interaction between phosphorylated STAT1 and interferon regulatory factor 9 (IRF9), which inactivated the interferons pathways and facilitated the malignant phenotype of HNSCC cells. Overexpression of STAT1 and IRF9 alleviated the malignant behavior caused by FAT1 inhibition. In summary, our study unveils the role of FAT1 in suppressing the CSCs properties of HNSCC via the CaMKII /STAT1/IRF9 pathway and targeting FAT1 might be a promising treatment for HNSCC.
Causal association between inflammatory bowel disease and type 2 diabetes: a bidirectional two-sample Mendelian randomization study
Authors: Yang Mengjiao , Ye Yongjuan , Zhang Meng , Chang Hong , Ouyang Jing , Zheng Ya , Wang Yuping , Guo Qinghong 
Published: October 2023

Abstract
Background
Previous observational studies have shown a paradoxical association between inflammatory bowel disease (IBD) and type 2 diabetes (T2DM), and the causal relationship between the two is unclear. Using a bidirectional two-sample Mendelian randomization (MR), we explored the causal connection between IBD and T2DM.
Methods
We obtained the datasets of IBD, UC, CD and T2DM from IEU genome-wide association study (GWAS) summary statistics and extracted genetic variants as instrumental variables for bidirectional two-sample MR. The main MR analysis methods are Inverse-variance weighted. Mendelian randomization-Egger (MR-Egger) and the weighted median as a complementary MR analysis method. Finally, heterogeneity tests, horizontal multiple validity tests, and the leave-one-out sensitivity analysis were used to evaluate the robustness of the study results.
Results
MR analysis results showed no causal connection between IBD (IVW OR = 1.017, 95%CI 0.981–1.053, p = 0.361), ulcerative colitis (UC) (IVW OR = 0.997, 95%CI 0.964–1.031, p = 0.864), Crohn’s disease (CD) (IVW OR = 1.018, 95%CI 0.986–1.052, p = 0.27) and T2DM. In addition, the results of the reverse MR analysis revealed no proof to support the risk of T2DM with the development of IBD (IVW OR = 1.049, 95%CI 0.898–1.225, p = 0.544), UC (IVW OR = 0.945, 95%CI 0.801–1.115, p = 0.501), and CD (IVW OR = 1.085, 95%CI 0.928–1.27, p = 0.307). Similar results were obtained for the complementary MR analysis method. The SNPs used in this study exhibited no conspicuous horizontal pleiotropy, and sensitivity analysis showed the robustness of the findings.
Conclusion
Our bidirectional two-sample MR results showed no evidence to support a causal connection between IBD, UC, CD and T2DM in the European population.