bluuebunny/crossref_metadata_2025_split huggingface dataset:
First large-scale study reveals important losses of honey bee and stingless bee colonies in Latin America
Authors: Requier Fabrice , Leyton Malena Sibaja , Morales Carolina L. , Garibaldi Lucas A. , Giacobino Agostina , Porrini Martin P. , Rosso-Londoño Juan Manuel , Velarde Rodrigo A. , Aignasse Andrea , Aldea-Sánchez Patricia , Allasino Mariana Laura , Arredondo Daniela , Audisio Carina , Cagnolo Natalia Bulacio , Basualdo Marina , Branchiccela Belén , Calderón Rafael A. , Castelli Loreley , Castilhos Dayson , Escareño Francisca Contreras , Correa-Benítez Adriana , Silva Fabiana Oliveira da , Garnica Diego Silva , de Groot Grecia , Delgado-Cañedo Andres , Fernández-Marín Hermógenes , Freitas Breno M. , Cardona Alberto Galindo , Garcia Nancy , Garrido Paula M. , Giray Tugrul , Gonçalves Lionel Segui , Landi Lucas , Gonçalves Daniel Malusá , Martinez Silvia , Moja Pablo Joaquín , Molineri Ana , Müller Pablo Fernando , Nogueira Enrique , Pacini Adriana , Palacio María Alejandra , Parra Guiomar Nates , Parra-H Alejandro , Gramacho Kátia Peres , Pérez Eleazar , Pires Carmen Sílvia Soares , Reynaldi Francisco J. , Luis Anais Rodríguez , Rossini Carmen , Armijos Milton Sánchez , Santos Estela , Scannapieco Alejandra , Spina Yamandú Mendoza , González José María Tapia , Fernández Andrés Marcelo Vargas , García Carlos Ariel Yadró , Viana Blandina Felipe , Vieli Lorena , Antúnez Karina 
Published: October 2023

Abstract

Over the last quarter century, increasing bee colony losses motivated standardized large-scale surveys of managed honey bees (Apis mellifera), particularly in Europe and the United States. Here we present the first large-scale standardized survey of colony losses of managed honey bees and stingless bees across Latin America. Overall, 1736 beekeepers and 165 meliponiculturists participated in the two-year survey (2016–2017 and 2017–2018). On average, 30.4% of honey bee colonies and 39.6% of stingless bee colonies were lost per year across the region. Summer losses were higher than winter losses in stingless bees (30.9% and 22.2%, respectively) but not in honey bees (18.8% and 20.6%, respectively). Colony loss increased with operation size during the summer in both honey bees and stingless bees and decreased during the winter in stingless bees. Furthermore, losses differed significantly between countries and across years for both beekeepers and meliponiculturists. Overall, winter losses of honey bee colonies in Latin America (20.6%) position in between the United States (40.4%) and Europe (12.5%). These results highlight the magnitude of bee colony losses occurring in the region and suggest difficulties in maintaining overall colony health and survival for beekeepers and meliponiculturists.
Performance Evaluation of Machine Learning Algorithms for Determining Influential Factors of Antenatal Care Visits in Bangladesh
Authors: Nayem H. M. , Hossain Md. Moyazzem , Aziz Sinha , Rahman Tareq , Trisha Salma Sultana , Bashar Abul 
Published: October 2023

Abstract
Antenatal care (ANC) is a crucial part of the ongoing care that a mother receives before and throughout her pregnancy, at the time of delivery, and during the recovery period. This study aimed to explore the influential factors of ANC visits and evaluate the predictive model performance of identifying the determinants of ANC visits in Bangladesh using seven machine learning algorithms. This study is based on the secondary data extracted from the 2017-2018 Bangladesh Demographic and Health Survey (BDHS), which covered a nationally representative sample of 20,250 ever-married women aged 15–49 years. The final data consist of 4,946 mothers who gave birth in the three years preceding the survey. Descriptive and inferential statistical techniques along with machine learning algorithms were used for data analysis. Of the 4,946 women, most were middle-aged and in the age groups of 20-24 years (35.4%) and 25-29 years (26.2%). Receiving a greater number of ANC services was significantly positively correlated with the frequency of ANC visits. Higher wealth indices increase the chance of completing an ANC visit. The random forest (RF) model shows that age, richest, number of children, household size, and mother's primary education level are the top five important predictors of antenatal care (ANC) visits. The quality of ANC services in Bangladesh could be increased by having a better grasp of the identified risk factors and implementing them in short- and long-term initiatives.
Factors Influencing Access to Primary Health Care in Luanda, Angola
Authors: Maquengo Antónia Sónia Carvalho , Bastos Alfredo Manuel , Santana Marli Stela 
Published: October 2023

Abstract

The Primary Health Care System (PHCS) is the first of the three hierarchical levels of care, based on the strategy of primary health care, is the first point of contact of the population with the health system. In Luanda, Angola, the primary care network presents challenges in its management, as well as in the perception of its purposes, which constitutes a serious problem for the demand for health services, this is the reason for this study. The present research aimed to evaluate the level of adherence and satisfaction related to health care system and their influence to acces the public primary health care facilities in Luanda, from the perspective of health professionals and users. A cross-sectional and descriptive study was carried out on 120 health professionals and 423 users. We found a statistically significant association between the level of education and the professionals' disbelief in health services (P = 0.001), as well as the users' family income (P = 0.0002). The users' perception is that there is a switch in test results (P = 0.01). Furthermore, they also believe that when attending health units, the user leaves sicker than he entered (P = 0.01). Thus, it was found that the level of user adherence to the services of the Primary Health Care Units (PHCU) in Luanda ranged between "good" and "acceptable".We identified elements capable of compromising the quality of services and, consequently, interfering with adherence to them, which suggests the need for the development of management strategies for health facilities, in order to overcome the challenges presented in the study.
Systematic review and meta-analysis of ACE insertion/deletion variant on cardiometabolic profile, premature coronary artery disease and severity of coronary lesions
Authors: Li Hang , 媛yuan Yuan , Peng Yuanyuan , Luo Zhi 
Published: October 2023

Abstract
Angiotensin-converting enzyme (ACE) is closely related to cardiometabolic risk factors and atherosclerosis. This study investigates whether the ACE variant impacts cardiometabolic profile, premature coronary artery disease (PCAD), and severity of coronary lesions. In total, 91 studies (94,270 individuals) were included for the analysis. The rs4646994 (an insertion/deletion variant in the ACE gene) D allele was linked to a higher levels of low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI), and waist circumference (WC). The D allele of rs4646994 was associated with a higher risk of PCAD and multiple vessel lesions. The impactsof rs4646994 on lipid levels were significant in Asians but stronger in females. In contrast, the impacts of rs4646994 on blood pressure, PCAD, and severity of coronary lesions were significant in Caucasians and males. Our study suggested that rs4646994 had a slight but significant impact on cardiometabolic risk factors, PCAD, and severity of coronary lesions. Angiotensin-converting enzyme inhibitors (ACEI) may benefit high-risk populations (eg, Caucasians, males, and females with high LDL-C levels) with rs4646994 to prevent PCAD and multiple vessel lesions.
Comorbidities of Diabetes and Hypertension in Vietnam: current burden, trends over time, and correlated factors
Authors: Vu Lan Thi Hoang , Bui Quyen Thi Tu , Tran Bao Quoc , Pham Nga Quynh , Lai Truong Duc , Le Hoang Tu , Hoang Minh Van 
Published: October 2023

Abstract
Background: Vietnam conducted the national STEPs surveys in the years 2010, 2015, and 2021. This study aims to use STEPs data to assess the burden of comorbidity between diabetes and hypertension, analyze trends over time, and identify factors associated with this comorbidity.
Methods: the study extracted data for the population aged 25-64 years old from three STEPs surveys. Survey weight was used for all estimations of prevalence and 95% CI. Cochran–Armitage test was used to test for significant trends. Correlated factors with comorbidity were examined by a multivariate logistics model.
Results: The prevalence of comorbidity in 2021 was about 3.92% among Vietnamese people aged 25-64. In the last 10 years, this prevalence has increased more than 8 times (from 0.44% to 3.92%). Sub-populations demonstrating the most significant changes included the male population, people living in urban areas, and older people. Significant factors correlated with comorbidity included demographic factors, BMI, and clustering of 4 NCD behavioral risk factors (OR=3.56, p<0.05)
Conclusion: The high comorbidity between hypertension and diabetes underscores the imperative for integrated treatment and management approaches in Vietnam. Coordinated care is essential for addressing the complex interplay between these two prevalent conditions.
Nutrition and diet myths, knowledge and practice during pregnancy and lactation among a sample of Egyptian pregnant women: A cross-sectional study
Authors: Abdalla Marwa , Zein Marwa M , Sherif Ahmed , Essam Bassam , Mahmoud Hend 
Published: October 2023

Abstract
Background
Globally, the burden of maternal malnutrition remains an enormous public health problem; malnourished pregnant women are at increased risk of having low-birth-weight (LBW) infants. Several reports suggest a possible association between malnutrition among lactating mothers and the production of smaller quantities of breast milk. Many women have incorrect nutrition knowledge during pregnancy due to false beliefs derived from popular practices. Our study was conducted to assess nutritional knowledge, myths, and practices among Egyptian women during pregnancy and lactation.
Methodology
A pretested 2-page interview questionnaire was used to collect data from the study participants after written informed consent was obtained from them after clarification of the study's aim. Obstetrics and gynecology experts collected the data from pregnant females who agreed to participate in private and university hospital antenatal care clinics in Cairo, Egypt.
Results
A total of 468 pregnant females completed the interview questionnaire. The mean knowledge score was 5 ± 3, with a median score of 5 and an IQR of 3–7, and the mean holding myths score was 3 ± 2, with a median score of 2 and an IQR of 2–4. Regarding the correct answers to the knowledge questions, more than 70% of the participants correctly answered that during the first six months of life, breast milk is the only food a baby requires, and less than 20% of them correctly answered that caffeine consumption could provoke premature birth. Regarding the holding myths questions, more than half of the participants held the myth that drinking moghat and helba increases the breast milk supply. We found that the most common source of knowledge during pregnancy and lactation among the participants was family and friends’ advice (60%), followed by others (doctors, previous education in school or university) (45%).
Conclusion
Among a sample of Egyptian women, more than half held at least one myth about nutrition and diet during pregnancy and breastfeeding, so health education at antenatal outpatient clinics should be directed toward those myths to correct them. Older women with sufficient family income showed significantly higher knowledge scores than others.
Study on predictive models for swallowing risk in patients with AECOPD
Authors: Xiong Shuyun , Zhou You , He Wenfeng , Zhu Jinling , He Wenfang , Ding Meizhu , Si Dongxu 
Published: October 2023

Abstract
BACKGROUND: The risk of swallowing is considered a complication in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). However, AECOPD may have risk factors for swallowing.
METHODS: This study included 100 patients with AECOPD. General Data, lung function, COPD assessment test (CAT) and Modified Medical Research Council Dyspnea Scale (mMRC) were collected. The questionnaire survey was conducted by uniformly trained investigators using standard and neutral language, and swallowing risk was evaluated on the day of patient admission.
RESULTS: Among the 100 included patients, 50(50%) were at risk of swallowing. Multivariate analysis using logistic regression analysis showed that age ≥ 74, mMRC≥2, hospitalization days≥7and the use of BIPAP assisted ventilation were important influencing factors for swallowing risk in patients with AECOPD.
CONCLUSION: Patients with AECOPD are at risk for swallowing, assessing age, mMRC, hospitalization days, and the use of BIPAP assisted ventilation can be used to screen for swallowing risk, thus contributing to the implementation of early prevention measures.
Cold Atmospheric Plasma Stabilizes Mismatch Repair for Effective, Uniform Treatment of Diverse Colorectal Cancer Cell Types
Authors: He Yuanyuan , Lu Fu , Jiang Chenmin , Gong Fanwu W. , Wu Zhengwei W. , Ostrikov Ken 
Published: October 2023

Abstract
Mismatch Repair (MMR) is a mechanism to repair DNA replication errors and could stabilize the DNA microsatellite structure. Colorectal cancer (CRC) can be divided into microsatellite stability (MSS) and microsatellite instability (MSI) types according to the functional difference of MMR. MSI colorectal cancer is more chemotherapy-resistant due to lower MMR protein expression. Cold atmospheric plasma (CAP) is an emerging treatment to induce apoptosis of various cancer cells. However, the therapeutic effects of CAP on MSI colorectal cancer and the underlying mechanisms remain unclear. Here, we studied the products and tools of CAP on MSI (MC38, HCT116, and LOVO) and MSS (CT26 and HT29) CRC cell lines. We found that CAP treatment showed the same effects on apoptosis, ROS, and RNS levels and expression of apoptosis-related proteins in both MSI and MSS cells. Mechanistically, CAP increased the expression of mismatch repair proteins (MLH1 and MSH2), particularly in MSI cells, which have been proven to promote apoptosis-related protein activation. Collectively, our study reveals that CAP upregulates apoptotic signaling and induces apoptosis in MSI colorectal cancer cells by increasing the expression of MMR proteins, thereby enhancing MMR stabilization.
Exploring combinations of dimensionality reduction, transfer learning, and regularization methods for predicting binary phenotypes with transcriptomic data
Authors: Oshternian Setareh Rezaee , Loipfinger Stefan , Bhattacharya Arkajyoti , Fehrmann Rudolf.S.N. 
Published: October 2023

Abstract

Background
Numerous transcriptomic-based models have been developed to predict or understand the fundamental mechanisms driving biological phenotypes. However, few models have successfully transitioned into clinical practice due to challenges associated with generalizability and interpretability. To address these issues, researchers have turned to dimensionality reduction methods and have begun implementing transfer learning approaches.
Methods
In this study, we aimed to evaluate the effectiveness of these strategies by exploring the optimal combination of dimensionality reduction methods (with and without transfer learning), and regularization techniques in predictive modeling. We employed four dimensionality reduction methods, namely, Principal Component Analysis (PCA), Consensus Independent Component Analysis (c-ICA), Autoencoder (AE), and Adversarial Variational Autoencoder (AVAE). Additionally, we applied a transfer learning approach by training the AE, AVEA, and c-ICA models on approximately 140,000 transcriptomic profiles. To assess the performance of the different combinations, we used a cross-validation setup encapsulated within a permutation testing framework, analyzing 30 different transcriptomic datasets with binary phenotypes. Furthermore, we included datasets with small sample sizes and phenotypes of varying degrees of predictability, and we employed independent datasets for validation.
Results
Our findings revealed that regularized models without dimensionality reduction achieved the highest predictive performance, challenging the necessity of dimensionality reduction when the primary goal is to achieve optimal predictive performance. However, models using AE and c-ICA with transfer learning for dimensionality reduction showed comparable performance, with enhanced interpretability and robustness of predictors, compared to models using non-dimensionality-reduced data.
Conclusion
These findings offer valuable insights into the optimal combination of strategies for enhancing the predictive performance, interpretability, and generalizability of transcriptomic-based models.
The danger of free roaming dogs in urban settings: A report of two suspected rabid dogs on rampage that inflicted 47 bites to people and domestic animals in peri-urban Uganda
Authors: Tayebwa Dickson , Rashid Njalira Kassim , Wilfred Eneku , Ssekandi Colin , Kyabarongo Alex , Mutesasira Jesse , Komugisha Mariam , Atim Stella Acaye , Kateregga John , Muhangi Denis , Tweyongyere Robert , Goldberg Tony Lawrence , Biryomumaisho Savino , Acai James Okwee 
Published: October 2023

Abstract
Background
Human rabies cases associated with bites from rabid roaming dogs are prevalent in Uganda. Unfortunately, there is limited documentation of the circumstances surrounding those bites. Here, we documented dog attacks to humans and animals in two peri-urban areas in Central Uganda.
Methods
We responded to alerts of two suspected rabid dogs on rampage. We traced the dogs responsible for the attacks to diagnose the cause of the aggression and we interviewed the victims and community members to determine the circumstances of the bites and health care sought by the victims. We scheduled follow-up visits after 40 days, to assess compliance of the victims to rabies postexposure prophylaxis (PEP) and to document any challenges encountered in the process.
Results
The two rabid dogs inflicted 47 recorded attacks to 29 humans, mostly children (75.8%) and 18 domestic animals in Matugga and Mawale parishes in Wakiso and Luweero districts, respectively. Two individuals washed the wound with water and 7 individuals reported to the health facility for treatment in accordance with the WHO-guidelines for preclinical management of bites from suspected rabies cases. By contrast, 17 individuals sought treatment from a traditional healer while 5 individuals self-medicated with herbs at home. We advised those individuals to visit health facilities for proper medical care. All the victims started rabies PEP treatment; however, 2 individuals did not complete their prescription. Although rabies PEP at public health facilities was free, regularly the victims paid 3–5 USD to the clinician as an incentive while the cost at private health facilities was 13–15 USD for a single dose. Due to the unprovoked attacks, the victims and local leaders advocated for elimination of roaming dogs, but the roaming dog owners objected.
Conclusions
Overall, our results demonstrate the outsized impact that a single-rabid dog can have on a community. They also reveal how poverty, knowledge gaps, community trust in traditional healers, and barriers to healthcare access negatively affect medical care given to dog bite victims in this setting. Educational campaigns and control of roaming dogs should help improve the situation in Central Uganda, as well as in other socioeconomically similar settings.
Role of Mast Cell, Ki-67 and Bcl-2 in the Diagnosis of Mycosis Fungoides
Authors: DAYE Munise , FINDIK Sıdıka , ISIK Begum , UYAR Mehmet 
Published: October 2023

Abstract
Diagnosis of early-stage MF, there are molecular studies that include Bcl-2 and ki-67, but there is no study showing that mast cells can be used both in the diagnosis of early-stage MF and in the etiopathogenesis of MF. In our study, we aimed to show that Ki-67, Bcl-2 and mast cell staining, which can be easily obtained in every laboratory, are helpful markers in the diagnosis of early-stage MF.
Methods:
A total of 81 cases, including 27 Mycosis Fungoides, 27 benign inflammatory dermatoses (psoriasis, lichen planus, eczema) and 27 patients without any disease, were included in the study retrospectively. Ki-67, Bcl-2 and mast cell counts were made under a light microscope with stained slides.
Results:
Mast cells were found to be significantly higher in cases of mycosis fungoides compared to cases of benign inflammatory dermatosis (p>0.001). In mycosis fungoides cases, ki-67, Bcl-2 and mast cells were significantly higher compared to the control group (p>0.001). In benign inflammatory dermatosis cases, ki-67 and Bcl-2 were found to be significantly higher than the control group (p>0.001).
Conclusion:
MF lesions are initially included in the differential diagnosis of many diseases including eczema, psoriasis, and lichen planus. In this case, histopathological examination is used in order to reach a clear diagnosis. Ki-67, Bcl-2 and mast cells were found significantly higher in MF patients compared to the control group. However, no difference was found between MF and benign inflammatory dermatoses in ki-67 and Bcl-2 staining.
Rupture of Thin Film Covering the Liquid Metal Stirred by Alternating Magnetic Field. Numerical Simulation
Authors: Nikulin I. L. , Demin V. A. , Nukulina S. A. 
Published: October 2023

Abstract
The present work deals with the technological process of induction melting of heat-resistant nickel alloys and, in particular, with the problem of formation and stability of oxide film on the surface of the melt. Our mathematical model describes the heat and mass transfer in the metal melt subjected to the influence of an alternating magnetic field, and the elastic stress states of the film on its surface. The governing equations of the problem are given and non-dimensional parameters are discussed. It is shown how the influence of the magnetic field strength on the flow of the metal melt changes at different frequencies. The states of the surface film are studied theoretically using numerical simulation. It is shown that the variation of the field strength at different frequencies leads to decaying oscillations in the velocity field. The causes of these oscillations are discussed in detail. The stress and destruction of thin films by melt motion are considered. The mechanisms responsible for the primary destruction of the initial film and its fragments are revealed. The rupture of quasi-stable films at different frequencies is studied. It is shown that the result of the influence on the film state with the increase of the magnetic field strength depends on the field frequency. The frequency range was found in which the surface film is strong enough to prevent the intensification of the melt flow with the increase of the magnetic field strength, this effect provides the stability of the film.