bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lyophilisation of bacterial test strains in a manifold-type apparatus: Effects of freezing and drying parameters, ampoule fill volume, and cotton filter density
Authors: Voropaev A. A. , Fadeikina O. V. , Ermolaeva T. N. , Davydov D. S.  
Published: October 2023

Scientific relevance. Lyophilisation is the preferred method at the National Collection of Pathogenic Microorganisms (NCPM) of the Scientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation. Lyophilisation is used to provide for high standards of test-strain deposition, storage, and transportation and to ensure that test strains maintain their properties. Successful lyophilisation requires conducting experiments to establish the key parameters and critical conditions of the process.Aim. The study aimed to evaluate the effects that the speed and time of freezing, the time of drying, the fill volume of ampoules, and the density of cotton filters have on the quality of NCPM indicator microorganisms lyophilised in a manifold-type apparatus.Materials and methods. Pseudomonas aeruginosa NCTC 12924, Staphylococcus aureus NCTC 10788, and Salmonella Abony NCTC 6017 were freeze-dried using a manifold-type apparatus (M. S. R. 18, Usifroid). The authors used a low-temperature freezer at –70±2 °C for slow freezing and a mixture of dry ice and alcohol for quick freezing. The statistical analysis was performed using Microsoft Excel and Statistica 10.Results. The minimum time needed for freezing the samples in a low-temperature freezer at –70±2 °C was 4 hours. Further storage at this temperature for up to 1 month was shown possible without compromising the quality of the final product. The time needed for freezing the samples in a mixture of dry ice and alcohol was under 1 minute. No differences in quality parameters were observed between the lyophilised samples frozen slowly or quickly, except for the cake appearance. Quick freezing resulted in cakes that were non-uniform, crumbled, and pulled away from the ampoule walls, which is considered undesirable. The primary drying stage for ampoules with a fill volume of 0.2 mL took 6–8 hours. The secondary drying stage of 11, 18, 35, and 59 hours resulted in comparable lyophilisate quality: the authors observed no statistically significant differences in viable cell counts (CFU/mL) at the end of lyophilisation and at the end of stress testing. The residual moisture content after 59-hour secondary drying was less than 2%. The cotton filter density had a critical influence on the lyophilisate quality. Therefore, the authors recommend using cotton filters weighing 50 mg or less.Conclusions. The authors analysed the main stages of the lyophilisation process used for NCPM test strains and considered the effects that the speed and time of freezing, the time of drying, the fill volume of ampoules, and the density of cotton filters have on the quality of the final lyophilised product. The NCPM has implemented the results of this study in its work.
Comparative safety evaluation of intranasal and intramuscular immunisation with Ad26 and Ad5-vectored vaccines to prevent coronavirus infection
Authors: Zuev E. V. , Evdokimova O. L. , Markova O. A. , Korotkevich I. A. , Grigorieva T. V. , Khamitov R. A. 
Published: October 2023

Scientific relevance. Intranasal vaccination may dramatically change current approaches to mass immunisation against various infections, shifting the focus from inducing systemic humoral and cellular immune responses to developing mucosal immunity and protective barriers on the mucous membranes, which are entry points for pathogens. Therefore, the safety of switching from intramuscular to intranasal vaccination should be evaluated.Aim. This study aimed to compare the safety of intranasal and intramuscular vaccination using the intermediate results of the phase III VCI-COV-III clinical trial in healthy volunteers.Materials and methods. The evaluation of the safety profile was based on the adverse events and adverse drug reactions (ADRs) documented by investigators in the interim report on the randomised, double-blind clinical trial of the Salnavac intranasal vaccine (InV) and the Gam-COVID-Vac intramuscular vaccine (ImV) against coronavirus infection. The report covered 42 days of observation in 137 healthy volunteers.Results. ADRs were reported for 17/68 (25%) and 30/69 (43.5%) volunteers in the InV group and the ImV group, respectively (p=0.036). The ADRs reported for the InV group were systemic and local, short-term, mostly mild, and generally consistent in incidence and nature with the ADRs typically observed with other nasal products.Conclusions. According to the preliminary data obtained in the clinical trial, the InV demonstrates a high level of safety. Its safety profile is comparable with those of other intranasal and intramuscular vaccines for the prevention of coronavirus infection.
THE EFFECT of PENCAK SILAT SPORTS on PREMENSTRUAL SYNDROME IN FEMALE STUDENTS of THE PENCAK SILAT STUDENT ACTIVITY UNIT at dr. SOEBANDI UNIVERSITY
Authors: Herlidian Putri  , Ai Nur Zannah  
Published: October 2023

The purpose of this study was to determine the effect of pencak silat exercise on premenstrual syndrome (PMS) in female students of UKM Pencak Silat.
This design is correlational analytic with cross sectional method. The population in study were all female members of UKM Pencak Silat University of dr. Soebandi Jember. The sample size in this study was 30. The statistical test is Spearman Rank.
There are a large proportion of respondents with sufficient pencak silat sports (56.7%). Most of the respondents (56.7%) did not experience Premenstrual Syndrome (PMS) in the mild category.
The results of the Spearman rank test obtained p value of 0.000 below the value of α (0.05), which means that Ha is accepted. .
There is an influence of pencak silat exercise on premenstrual syndrome (PMS) in female students of UKM Pencak Silat University, dr. Soebandi Jember
Organização e orientação do cuidado em Saúde Mental na Atenção Primária à Saúde: uma análise documental
Authors: Calazans de Araújo Suellen , Feijó da Silva Tarciso , Sousa dos Santos Roberta Georgia , Guimarães de Araújo Faria Magda , Tavares Vieira Ana Carolina , Roza Dias Juliana , Oliveira de Andrade Paquiela Eliane , Cabral da Silva Ramos Tatiana 
Published: October 2023

Objetivo: Analisar as legislações e normativas que organizam e orientam o cuidado em saúde mental na Rede de Atenção Psicossocial no Sistema Único de Saúde. Métodos: Estudo descritivo e exploratório delineado pela análise documental das legislações e normativas oficiais que versam sobre a saúde mental e que emergiram a partir da Lei 10.216 de 2001 que redireciona o modelo de atenção à saúde mental no Brasil. Os documentos foram coletados nos portais da Secretaria de Atenção Primária à Saúde e no Sistema de Legislação da Saúde do Ministério da Saúde e da Subsecretaria de Promoção, Atenção Primária e Vigilância em Saúde do Município do Rio de Janeiro. Resultados: Foram identificadas 27 publicações que apresentam diferentes formas de organização do cuidado em saúde mental e dispositivos que podem ser utilizados na gestão da atenção e no cuidado integral em saúde mental na Atenção Primária à Saúde. Considerações finais: Os documentos possuem caráter prescritivo, sendo capazes de contribuir para orientação e organização do cuidado em saúde mental. No entanto, na prática permanecem impasses associados ao desmonte das políticas de saúde mental alinhadas com a Reforma Psiquiátrica Brasileira, despreparo do profissional técnico na condução dos casos, a frágil comunicação entre os pontos da rede, o matriciamento incipiente e a intensa medicalização da vida e das pessoas.
Palavras-chave: Saúde Mental; Políticas Públicas de Saúde; Atenção Primária à Saúde; Enfermagem.
THE RELATIONSHIP BETWEEN GRUDGE MOOD AND LIFE SATISFACION IN UNIVERSITY STUDENTS
Authors: TİRYAKİ GÖKSU Yeliz , ERSANLI Kurtman 
Published: October 2023

The aim of this study is to investigate the relationship between grudge mood in university students and their satisfaction with life. The study group consisted of a total of 520 university students, 320 of whom were female and 200 of whom were male. The study was carried out in two steps. In the first step, a development study was carried out to measure the grudge mood in individuals using the ‘Grudge Mood Scale." For this purpose, a item pool was created with the views of relevant literature and field workers. The created items were appilied to 335 university students and analyzed with Exploratory Factor Analysis. According to the Exploratory Factor Analysis, the scale consists of four sub-dimensions. This four-dimensional structure it was applied to 350 students and analyzed by Confirmatory Factor Analysis. The results of confirmatory factor analysis have shown that the fit indices of the scale indicate a good fit with a four-factor structure. To test the validity and reliability of the developed scale, internal consistency coefficient, split-half reliability, content validity, test-retest reliability, and scale-related validity analyses were conducted. As a result of the analyses, it was concluded that the developed 'Grudge Mood Scale' is a valid and reliable scale.
In the second step of the research, the relationship between grudge mood and life satisfaction was examined. Grudge Mood Scale, Life Satisfaction Scale and Personal Information Form were used as data collection tools. After the normality examination of the data, the Pearson Moment Product Correlation and Multivariate Variance Analysis techniques were used. According to the results of the analysis, there is a negative and significant relationship between university students' grudge mood and life satisfaction. Furthermore, the relationship between grudge mood and life satisfaction varies significantly according to the students' genders. The findings obtained from this study were interpreted in the context of the literature and recommendations were made.
Local Pottery in the Lower Bug Region at the Early Stages of Urbanization
Authors: Kotenko Viktoriia , Kushnir Anatolii , Smyrnov Oleksandr 
Published: October 2023

The purpose of the paper is to investigate the development of pottery in the Lower Bug region and its role in urbanization processes on the basis of historiographical, archaeological and paleogeographical data at the 6th century BCE.
Scientific novelty. For the first time the ceramic production are comprehensively considered into account the raw material base of the pottery of the ancient settlement on the example of Borysthenes. The relationship between social and natural factors in the development of pottery has been established. The role of this craft in the context of urbanization processes in the North-Western Black Sea region is determined. The early production of ceramics in Lower Bug is considered, covering different categories of sources. A method of studying the local pottery making in the ancient centres of the region is proposed.
Methodology. The study was based on a comprehensive analysis of historiographical and archaeological data and paleogeographical methods. The field stage of the research included the survey of sites with unsoddy deposits of various geological periods as potential pottery raw materials. A stratigraphic dissection of deposits, their macromorphological description and samples were taken from each sites for further laboratory research. The laboratory research included a micromorphological analysis of ceramic products to compare its characteristics with potential raw materials, as well as a granulometric analysis of the selected samples to determine their physicochemical properties.
Conclusions. Pottery as an independent type of craft was distinguished in Hellenic culture quite early and came to the centres of the Northern Black Sea region as an already formed occupation. Spatially, workshops were situated at the large cities, where the manufactured products not only satisfied local demand, but also served as goods for sale. As archaeological materials showed, the earliest evidence of local pottery production was discovered on Berezan island and date from the middle of the 6th century BCE. The active development of the settlement, i.e. the intensification of the urbanization of the space, belongs around the same period. Therefore, the development of crafts, in particular pottery, is a component of this multifaceted process.
Paleopedological studies, micromorphological and granulometric analyzes of potential pottery raw materials with the micromorphological features of ceramics allow us to talk about local production. It was found that craftsmen could use local raw materials to create the moulding mass, which was obviously multi-component. The materials mainly of the first half of the 6th century BCE from site “T” of Berezan settlement confirmed the assumption about the predominance of grey ceramics (“greyware”) in the local pottery making at that time. Probably, the tradition of its production existed for a long time.
Doctors of the Roman garrison of Tyras
Authors: Ivantchik Askold 
Published: October 2023

The article revises the readings of two inscriptions of Tyras which presumably contain information about the organization of the medical service in the Roman garrison of that city in the 2nd century AD. The first of these (L’Année épigraphique, 1995, 1350) confirms the mention of medicus vexillationis, a term unknown in other texts; the inscription belongs to the very few evidences of the existence of the position of doctor of vexillation. It confirms the existence of at least two doctors in the vexillation of Tyras, one of whom was a naval doctor. The inscription is the only evidence so far to show that doctors serving in the provincial, and not only praetorian (Misene and Ravennate) fleets had the status of duplicarius.
However, the inscription itself turns out to be of a different nature than its first publisher P.O. Karyshkovsky had suggested – it is not an honorary inscription, but a dedication to Asclepius and Hygia on behalf of not only two doctors, but also two legionaries, presumably privates. The text of the first three lines is reconstructed differently: [D(eo) A]ṣclepio • et • Hygi(ae) | [e.g. T(itus) • Fl(avius) •] Ṣuccessus • M(arcus) • Ata|[eus? • P]ḷacid[(us) m(ilites)] ḷeg(ionis) • Ī • Ital(icae).
In the second inscription (L’Année épigraphique, 1990, 869) the mention of the unique term valetudinarius, usually interpreted as referring to personnel of hospital (valetudinarium), is not confirmed. In its place the cognomen of one of the dedicants Valens is to be restored and the term itself must be considered a “ghost”.
Circular economy-based multi-objective decentralized controller for activated sludge wastewater treatment plant
Authors: Subbian Sutha , Natarajan Pappa , Murugan Chitra 
Published: October 2023

Introduction: Water scarcity and water pollution are two major issues in India. Circular economy-based wastewater treatment technology provides the most sustainable solutions for solving these issues. In this paper, a novel multi-objective decentralized controller (MODC) is proposed for benchmarking a multi-input multi-output (MIMO) activated sludge wastewater treatment plant (WWTP) to achieve maximum effluent quality with minimum cost. WWTPs with conventional control schemes consume more energy to achieve the desired effluent quality.Methods: In this study, a MIMO model is developed for the activated sludge process (ASP) from a physics-based model, and relative gain array (RGA) analysis are carried out to determine the interaction between the loops to identify a suitable control scheme for the MIMO process. In addition, a multi-objective decentralized control problem is formulated to achieve the conflicting multiple objectives of improving effluent quality and minimizing operational costs by efficient usage of energy.Results and discussion: The desired quality and cost reduction are verified by comparing the integral square error (ISE) and control effort (CE) values of a closed-loop WWTP. A multi-objective evolutionary algorithm (MOEA), namely, the non-dominated sorting genetic algorithm (NSGA)-II, successfully solves the multi-objective control problem. NSGA-II provides several optimal solutions in the Pareto front. In order to demonstrate the feasibility of the proposed controller, three optimal solutions are selected from the Pareto-optimal front, and their closed-loop performances are evaluated qualitatively and quantitatively for both servo and regulatory operations. Improving the quality of effluent enhances active sludge production, which in turn increases the methane production in the anaerobic digester.
Determinants of vascular structure and function in at-risk children born to mothers managed for pre-eclampsia (FINNCARE study)
Authors: Renlund Michelle , Jääskeläinen Tiina , Kivelä Anni , Heinonen Seppo , Laivuori Hannele , Sarkola Taisto 
Published: October 2023

Background and aimPre-eclampsia (PE) is related to elevated blood pressure (BP) in children. The study aims to investigate if elevated BP is reflected in child arterial health and how anthropometrics, body composition, and gestational and perinatal factors influenced this.MethodsIn this prospective cohort study, we assessed the arteries of 182 children exposed (46 had an early onset, with a diagnosis before 34 gestational weeks, and 136 had a late onset) and 85 children unexposed (non-PE) to PE at 8–12 years from delivery using ultra-high-frequency ultrasound in addition to ambulatory and central BPs, body composition and anthropometrics, and tonometry-derived pulse wave velocity (PWV).ResultsNo differences were found in intima-media thickness (IMT), adventitia thickness (AT), lumen diameter (LD), local carotid artery stiffness, distensibility, or wall stress between PE-exposed and non-PE-exposed children. All children's brachial, radial, and femoral artery IMTs were associated with 24-h systolic BP (SBP) and pulse pressure, carotid–femoral PWV, and anthropometric measures. The 24-h SBP and anthropometrics, notably lean body mass, were independent predictors of peripheral artery IMTs (brachial R2 = 0.217, radial R2 = 0.208, femoral R2 = 0.214; p &lt; 0.001). Head circumference predicted carotid artery IMT and LD (β = 0.163, p = 0.009; β = 0.417, p &lt; 0.001, respectively), but carotid artery IMT was not associated with BP. No independent associations were found for peripheral artery ATs. Local carotid artery stiffness, distensibility, and wall stress were independently associated with adiposity. No significant associations were found between gestational or perinatal factors and child vascular health parameters.ConclusionsThe peripheral artery IMT of PE-exposed children is identical to that of non-PE-exposed children, but associated with BP. Adiposity is related to local carotid artery stiffness. These adverse associations in arterial health may reflect the early progression of cardiovascular disease in PE-exposed children.
Adaptation of the Gabby conversational agent system to improve the sexual and reproductive health of young women in Lesotho
Authors: Nkabane-Nkholongo Elizabeth , Mokgatle Mathildah , Bickmore Timothy , Julce Clevanne , Jack Brian W. 
Published: October 2023

IntroductionYoung women from the low-middle-income country of Lesotho in southern Africa frequently report limited knowledge regarding sexual and reproductive health issues and engage in risky sexual behaviors. The purpose of this study is to describe the adaptation of an evidence-based conversational agent system for implementation in Lesotho and provide qualitative data pertaining to the success of the said adaptation.MethodsAn embodied conversational agent system used to provide preconception health advice in the United States was clinically and culturally adapted for use in the rural country of Lesotho in southern Africa. Inputs from potential end users, health leaders, and district nurses guided the adaptations. Focus group discussions with young women aged 18–28 years who had used the newly adapted system renamed “Nthabi” for 3–4 weeks and key informant interviews with Ministry of Health leadership were conducted to explore their views of the acceptability of the said adaptation. Data were analyzed using NVivo software, and a thematic content analysis approach was employed in the study.ResultsA total of 33 women aged 18–28 years used Nthabi for 3–4 weeks; eight (24.2%) of them were able to download and use the app on their mobile phones and 25 (75.8%) of them used the app on a tablet provided to them. Focus group participants (n = 33) reported that adaptations were culturally appropriate and provided relevant clinical information. The participants emphasized that the physical characteristics, personal and non-verbal behaviors, utilization of Sesotho words and idioms, and sensitively delivered clinical content were culturally appropriate for Lesotho. The key informants from the Ministry leadership (n = 10) agreed that the adaptation was successful, and that the system holds great potential to improve the delivery of health education in Lesotho. Both groups suggested modifications, such as using the local language and adapting Nthabi for use by boys and young men.ConclusionsClinically tailored, culturally sensitive, and trustworthy content provided by Nthabi has the potential to improve accessibility of sexual and reproductive health information to young women in the low-middle-income country of Lesotho.