bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of M1 cholinergic receptors in mouse somatosensory cortex enhances information processing and detection behaviour
Authors: Mishra Wricha , Kheradpezhouh Ehsan , Arabzadeh Ehsan 
Published: January 2024

AbstractTo optimise sensory representations based on environmental demands, the activity of cortical neurons is regulated by neuromodulators such as Acetylcholine (ACh). ACh is implicated in cognitive functions including attention, arousal and sleep cycles. However, it is not clear how specific ACh receptors shape the activity of cortical neurons in response to sensory stimuli. Here, we investigate the role of a densely expressed muscarinic ACh receptor M1 in information processing in the mouse primary somatosensory cortex and its influence on the animal’s sensitivity to detect vibrotactile stimuli. We show that M1 activation results in faster and more reliable neuronal responses, manifested by a significant reduction in response latencies and the trial-to-trial variability. At the population level, M1 activation reduces the network synchrony, and thus enhances the capacity of cortical neurons in conveying sensory information. Consistent with the neuronal findings, we show that M1 activation significantly improves performances in a vibriotactile detection task.
Synchronized LFP rhythmicity in the social brain reflects the context of social encounters
Authors: Mohapatra Alok Nath , Peles David , Netser Shai , Wagner Shlomo 
Published: January 2024

AbstractMammalian social behavior is highly context-sensitive. Yet, little is known about the mechanisms that modulate social behavior according to its context. Recent studies have revealed a network of mostly limbic brain regions which regulates social behavior. We hypothesize that coherent theta and gamma rhythms reflect the organization of this network into functional sub-networks in a context-dependent manner. To test this concept, we simultaneously record local field potential (LFP) from multiple social brain regions in adult male mice performing three social discrimination tasks. While LFP rhythmicity across all tasks is dominated by a global internal state, the pattern of theta coherence between the various regions reflect the behavioral task more than other variables. Moreover, Granger causality analysis implicate the ventral dentate gyrus as a main player in coordinating the context-specific rhythmic activity. Thus, our results suggest that the pattern of coordinated rhythmic activity within the network reflects the subject’s social context.
Proteolytic activation of fatty acid synthase signals pan-stress resolution
Authors: Wei Hai , Weaver Yi M. , Yang Chendong , Zhang Yuan , Hu Guoli , Karner Courtney M. , Sieber Matthew , DeBerardinis Ralph J. , Weaver Benjamin P. 
Published: January 2024

AbstractChronic stress and inflammation are both outcomes and major drivers of many human diseases. Sustained responsiveness despite mitigation suggests a failure to sense resolution of the stressor. Here we show that a proteolytic cleavage event of fatty acid synthase (FASN) activates a global cue for stress resolution in Caenorhabditis elegans. FASN is well established for biosynthesis of the fatty acid palmitate. Our results demonstrate FASN promoting an anti-inflammatory profile apart from palmitate synthesis. Redox-dependent proteolysis of limited amounts of FASN by caspase activates a C-terminal fragment sufficient to downregulate multiple aspects of stress responsiveness, including gene expression, metabolic programs and lipid droplets. The FASN C-terminal fragment signals stress resolution in a cell non-autonomous manner. Consistent with these findings, FASN processing is also seen in well-fed but not fasted male mouse liver. As downregulation of stress responses is critical to health, our findings provide a potential pathway to control diverse aspects of stress responses.
UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation
Authors: Ahlstedt Brittany A , Ganji Rakesh , Mukkavalli Sirisha , Paulo Joao A , Gygi Steve P , Raman Malavika 
Published: January 2024

AbstractER protein homeostasis (proteostasis) is essential for proper folding and maturation of proteins in the secretory pathway. Loss of ER proteostasis can lead to the accumulation of misfolded or aberrant proteins in the ER and triggers the unfolded protein response (UPR). In this study, we find that the p97 adaptor UBXN1 is an important negative regulator of the UPR. Loss of UBXN1 sensitizes cells to ER stress and activates the UPR. This leads to widespread upregulation of the ER stress transcriptional program. Using comparative, quantitative proteomics we show that deletion of UBXN1 results in a significant enrichment of proteins involved in ER-quality control processes including those involved in protein folding and import. Notably, we find that loss of UBXN1 does not perturb p97-dependent ER-associated degradation (ERAD). Our studies indicate that loss of UBXN1 increases translation in both resting and ER-stressed cells. Surprisingly, this process is independent of p97 function. Taken together, our studies have identified a new role for UBXN1 in repressing translation and maintaining ER proteostasis in a p97 independent manner.
LncRNA DANA1 promotes drought tolerance and histone deacetylation of drought responsive genes in Arabidopsis
Authors: Cai Jingjing , Zhang Yongdi , He Reqing , Jiang Liyun , Qu Zhipeng , Gu Jinbao , Yang Jun , Legascue María Florencia , Wang Zhen-Yu , Ariel Federico , Adelson David L , Zhu Youlin , Wang Dong 
Published: January 2024

AbstractAlthough many long noncoding RNAs have been discovered in plants, little is known about their biological function and mode of action. Here we show that the drought-induced long intergenic noncoding RNA DANA1 interacts with the L1p/L10e family member protein DANA1-INTERACTING PROTEIN 1 (DIP1) in the cell nucleus of Arabidopsis, and both DANA1 and DIP1 promote plant drought resistance. DANA1 and DIP1 increase histone deacetylase HDA9 binding to the CYP707A1 and CYP707A2 loci. DIP1 further interacts with PWWP3, a member of the PEAT complex that associates with HDA9 and has histone deacetylase activity. Mutation of DANA1 enhances CYP707A1 and CYP707A2 acetylation and expression resulting in impaired drought tolerance, in agreement with dip1 and pwwp3 mutant phenotypes. Our results demonstrate that DANA1 is a positive regulator of drought response and that DANA1 works jointly with the novel chromatin-related factor DIP1 on epigenetic reprogramming of the plant transcriptome during the response to drought.
FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability
Authors: Boavida Ana , Napolitano Luisa MR , Santos Diana , Cortone Giuseppe , Jegadesan Nanda K , Onesti Silvia , Branzei Dana , Pisani Francesca M 
Published: January 2024

AbstractFANCJ, a DNA helicase linked to Fanconi anemia and frequently mutated in cancers, counteracts replication stress by dismantling unconventional DNA secondary structures (such as G-quadruplexes) that occur at the DNA replication fork in certain sequence contexts. However, how FANCJ is recruited to the replisome is unknown. Here, we report that FANCJ directly binds to AND-1 (the vertebrate ortholog of budding yeast Ctf4), a homo-trimeric protein adaptor that connects the CDC45/MCM2-7/GINS replicative DNA helicase with DNA polymerase α and several other factors at DNA replication forks. The interaction between FANCJ and AND-1 requires the integrity of an evolutionarily conserved Ctf4-interacting protein (CIP) box located between the FANCJ helicase motifs IV and V. Disruption of the CIP box significantly reduces FANCJ association with the replisome, causing enhanced DNA damage, decreased replication fork recovery and fork asymmetry in cells unchallenged or treated with Pyridostatin, a G-quadruplex-binder, or Mitomycin C, a DNA inter-strand cross-linking agent. Cancer-relevant FANCJ CIP box variants display reduced AND-1-binding and enhanced DNA damage, a finding that suggests their potential role in cancer predisposition.
Shaping immune landscape of colorectal cancer by cholesterol metabolites
Authors: Bai Yibing , Li Tongzhou , Wang Qinshu , You Weiqiang , Yang Haochen , Xu Xintian , Li Ziyi , Zhang Yu , Yan Chengsong , Yang Lei , Qiu Jiaqian , Liu Yuanhua , Chen Shiyang , Wang Dongfang , Huang Binlu , Liu Kexin , Song Bao- Liang , Wang Zhuozhong , Li Kang , Liu Xin , Wang Guangchuan , Yang Weiwei , Chen Jianfeng , Hao Pei , Zhang Zemin , Wang Zhigang , Zhu Zheng-Jiang , Xu Chenqi 
Published: January 2024

AbstractCancer immunotherapies have achieved unprecedented success in clinic, but they remain largely ineffective in some major types of cancer, such as colorectal cancer with microsatellite stability (MSS CRC). It is therefore important to study tumor microenvironment of resistant cancers for developing new intervention strategies. In this study, we identify a metabolic cue that determines the unique immune landscape of MSS CRC. Through secretion of distal cholesterol precursors, which directly activate RORγt, MSS CRC cells can polarize T cells toward Th17 cells that have well-characterized pro-tumor functions in colorectal cancer. Analysis of large human cancer cohorts revealed an asynchronous pattern of the cholesterol biosynthesis in MSS CRC, which is responsible for the abnormal accumulation of distal cholesterol precursors. Inhibiting the cholesterol biosynthesis enzyme Cyp51, by pharmacological or genetic interventions, reduced the levels of intratumoral distal cholesterol precursors and suppressed tumor progression through a Th17-modulation mechanism in preclinical MSS CRC models. Our study therefore reveals a novel mechanism of cancer–immune interaction and an intervention strategy for the difficult-to-treat MSS CRC.
Gasdermin D in macrophages drives orchitis by regulating inflammation and antigen presentation processes
Authors: Ma Chunmei , Huang Jiajia , Jiang Yuying , Liu Lu , Wang Na , Huang Shaoqiong , Li Honghui , Zhang Xiangyu , Wen Shuang , Wang Bingwei , Yang Shuo 
Published: January 2024

AbstractInflammation in the testes induced by infection and autoimmunity contributes significantly to male infertility, a public health issue. Current therapies using antibiotics and broad-spectrum anti-inflammatory drugs are ineffective against non-bacterial orchitis and induce side effects. This highlights the need to explore the pathogenesis of orchitis and develop alternative therapeutic strategies. In this study, we demonstrated that Gasdermin D (GSDMD) was activated in the testes during uropathogenic Escherichia coli (UPEC)-induced acute orchitis, and that GSDMD in macrophages induced inflammation and affected spermatogenesis during acute and chronic orchitis. In testicular macrophages, GSDMD promoted inflammation and antigen presentation, thereby enhancing the T-cell response after orchitis. Furthermore, the pharmacological inhibition of GSDMD alleviated the symptoms of UPEC-induced acute orchitis. Collectively, these findings provide the first demonstration of GSDMD’s role in driving orchitis and suggest that GSDMD may be a potential therapeutic target for treating orchitis.
Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema
Authors: Creff Justine , Lamaa Asalaa , Benuzzi Emeline , Balzan Elisa , Pujol Francoise , Draia-Nicolau Tangra , Nougué Manon , Verdu Lena , Morfoisse Florent , Lacazette Eric , Valet Philippe , Chaput Benoit , Gross Fabian , Gayon Regis , Bouillé Pascale , Malloizel-Delaunay Julie , Bura-Rivière Alessandra , Prats Anne-Catherine , Garmy-Susini Barbara 
Published: January 2024

AbstractSecondary lymphedema (LD) corresponds to a severe lymphatic dysfunction leading to the accumulation of fluid and fibrotic adipose tissue in a limb. Here, we identified apelin (APLN) as a powerful molecule for regenerating lymphatic function in LD. We identified the loss of APLN expression in the lymphedematous arm compared to the normal arm in patients. The role of APLN in LD was confirmed in APLN knockout mice, in which LD is increased and associated with fibrosis and dermal backflow. This was reversed by intradermal injection of APLN-lentivectors. Mechanistically, APLN stimulates lymphatic endothelial cell gene expression and induces the binding of E2F8 transcription factor to the promoter of CCBE1 that controls VEGF-C processing. In addition, APLN induces Akt and eNOS pathways to stimulate lymphatic collector pumping. Our results show that APLN represents a novel partner for VEGF-C to restore lymphatic function in both initial and collecting vessels. As LD appears after cancer treatment, we validated the APLN-VEGF-C combination using a novel class of nonintegrative RNA delivery LentiFlash® vector that will be evaluated for phase I/IIa clinical trial.
Silver(i) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(i) ion
Authors: Stanković Mia , Kljun Jakob , Stevanović Nevena Lj. , Lazic Jelena , Skaro Bogojevic Sanja , Vojnovic Sandra , Zlatar Matija , Nikodinovic-Runic Jasmina , Turel Iztok , Djuran Miloš I. , Glišić Biljana Đ. 
Published: January 2024

The clinically used antifungal agents, econazole, voriconazole and clotrimazole, were successfully applied in the synthesis of novel silver(i)-azole complexes with significantly improved anti-Candida activity in respect to the parent organic drugs.
Climate change performance of hydrogen production based on life cycle assessment
Authors: Patel Gulam Husain , Havukainen Jouni , Horttanainen Mika , Soukka Risto , Tuomaala Mari 
Published: January 2024

Green H2 production reduces emissions by 80–95% compared to grey H2 thus meeting the REDII directive. However, blue and turquoise H2 fall short of meeting this criterion without CO2 sequestration.
Alumina-Promoted Copper-Catalyzed Hydroboration of Alkynes
Authors: Latyshev Gennadij V. , Barashkova Xenia A. , Parulava Mikhail J. , Kotovshchikov Yury N. , Lukashev Nikolay V. , Beletskaya Irina P. 
Published: January 2024

AbstractAlumina was found to activate Cu(I) catalysts in hydroboration of alkynes. This allowed to develop a simple and efficient protocol for hydroboration of alkynes with B2Pin2 using inexpensive CuCl/PPh3 catalytic system. The approach does not require moisture-sensitive activators and stepwise preparation of ‘activated’ Cu complex. The developed conditions were applied to the preparation of β-styrylboronates from terminal and internal alkynes with excellent regio- and stereoselectivity. The procedure can also be extended to other terminal alkynes by the appropriate choice of ligand.
Corporate social responsibility and green supply chain efficiency: conditioning effects based on CEO narcissism
Authors: Liao Feimei , Hu Yaoyao , Ye Songqin 
Published: January 2024

AbstractAccelerating the construction of the green supply chain system and improving the efficiency of the green supply chain is the key to promoting the high-quality development of enterprises. In view of this, based on stakeholder theory, higher order theory and expectancy theory, this study focuses on the impact of corporate social responsibility (CSR) on corporate green supply chain efficiency (GSCE) and the moderating role of chief executive officer (CEO) narcissism. A regression analysis of the observed sample reveals that CSR significantly enhances GSCE. Further decomposing CSR into internal CSR and external CSR to reveal the impact of different types of CSR on GSCE, we find that internal CSR fulfillment has a significant positive impact on GSCE, and this relationship is strengthened when CEOs are narcissistic. Furthermore, external CSR has a significant negative impact on GSCE, and this relationship is also strengthened by CEO narcissism. The main contribution of this paper is to study the relationship between CSR and green supply chain efficiency, decompose CSR into internal and external CSR, enrich the research on the intrinsic mechanism of value creation of CSR. It also enriches the research in the context of CSR from the perspective of CEO personality traits, providing new ideas and suggestions for manager selection and corporate greening governance in practice.
Mineral and bioresource exploitation for transformation and sustainability of the chemical industry in Zambia
Authors: Nyirenda James , Malabo Harriet 
Published: January 2024

AbstractZambia depends mainly on copper mining and imports the bulk of chemicals needed for academic, domestic, and industrial consumption. The country has never had an economically viable chemical industry despite having vast minerals and bioresources key to the stimulation of a sustainable chemical industry. This paper proposes a theory of change model for the establishment of the chemical industry in Zambia to enhance economic growth. Availability of minerals and bioresources have been assumed as a strong driver to establish an economically viable chemical industry in Zambia. The paper discusses the economic status of Zambia, explains current economic drivers and proposes establishment of a chemical industry as a critical missing link to transform the economy. Briefly a thorough literature desk review was done with cue search words such as “Chemistry professionals Zambia”, “Policy Chemicals industry Zambia”, “Chemical industry Zambia”, “National Development Plan Zambia”, “Petrochemicals Zambia”, “Specialty chemicals Zambia”, “Gross Domestic product Zambia” in the Google scholar search engine. Results revealed that exclusion of chemical industry in policy formulation and implementation, lack of political will in promoting chemistry, underutilization of available mineral and bioresources remains part of the main reason for lack of growth of the chemical industry and serial failure of the seven national development plans to adequately plan for this since independence in 1964. The period from 1961 to 2021 was considered and the 2006 to 2021 comprising the fifth, sixth and seventh national development plans was isolated, and analysis of variance done for the three plans with respect to gross domestic product (GDP) and GDP per capita. There was a statistical significance for both parameters. A p = 0.0004 for GDP and 0.0003 for GDP per capita values showed that there was negative or almost no growth within each period of the national development plan. None of the seven development plans addressed a deliberate setting up of the chemical industry to reduce capital flight in terms of huge imports. Hence, we opine that the chemical industry in Zambia is desperately needed to provide agrochemicals and other specialty chemical feedstocks.
Willingness to pay for pesticide-free vegetables in Hokkaido, Japan: the relationship between appearance and pesticide use
Authors: Nohara Katsuhito 
Published: January 2024

AbstractMost Japanese consumers focus on the appearance of vegetables, for example, their shape, color, or size. However, giving consumers precise information about the growing process of vegetables may change their preferences. Therefore, in order to reduce food loss, it is important to change consumers’ preferences for vegetables and for producers to understand consumers’ preferences and select vegetable production methods. Japanese supermarkets sell vegetables containing legally permissible amounts of pesticides and chemical fertilizers, and chemical-free vegetables are generally hard to find. Therefore, in this study, pesticide-free vegetables were cultivated from scratch to analyze whether consumers are willing to pay a premium when presented with pesticide-free vegetables. The contingent valuation method and double-bound dichotomous choice were used for analysis. The results revealed that consumers’ willingness to pay was not related to the appearance of the pesticide-free vegetables. In other words, they cared neither about the color nor the shape of the vegetables so long as they were pesticide-free. In Japan, some farmers continue to grow organic or pesticide-free vegetables, although they are few. Therefore, if producers know the actual needs of consumers’ vegetables, they may reduce their dependence on pesticides and chemical fertilizers and expand the market for environmentally friendly and healthy vegetables. Such a change could, in turn, reduce food loss.
Examining factors influencing the user’s loyalty on algorithmic news recommendation service
Authors: Lim Chulmin , Kim Seongcheol 
Published: January 2024

AbstractBased on user-related data, an algorithmic news recommendation service (ANRS) predicts users’ reading preferences and selectively recommends news. Given the double-edged opinions on ANRS, identifying and managing crucial factors influencing users’ satisfaction and trust in this service will be essential for service providers and developers. However, few studies have tried to find these factors or develop a more precise understanding of users’ perceptions of this service. Therefore, this study aims to examine factors affecting users’ loyalty to ANRS with partial least squares structural equation modelling (PLS-SEM). This study conducted an online survey for users of “My News”, the free mobile ANRS of NAVER, Korea’s dominant online portal site, and analyzed the data from 483 responses. This analysis verified that both satisfaction and trust positively affect loyalty to ANRS, and trust positively affects satisfaction. Moreover, it was found that perceived accuracy positively affects satisfaction. The result also showed that perceived news value and perceived transparency positively affect trust, and privacy concerns negatively affect it. Lastly, it was found that perceived usability and pre-existing attitude toward the service provider positively affect satisfaction and trust. The results and discussions will be helpful for service providers and developers to manage ANRS effectively based on users’ responses and perceptions of this service.
Is visual content modality a limiting factor for social capital? Examining user engagement within Instagram-based brand communities
Authors: Kusuma Agung Artha , Afiff Adi Zakaria , Gayatri Gita , Hati Sri Rahayu Hijrah 
Published: January 2024

AbstractIn the age of virtual cocreation of value by consumers, the role of the content modality in the development of social capital has been largely overlooked. Given that different modalities lead to varied forms of digital communication, this study examines whether a predominantly visual modality can enhance social capital and improve the collective value perceived by members of an online brand community. Through quantitative analysis, this study demonstrates that the visual modality of Instagram fosters social interactions, shaping the platform’s engagement dynamics. Affect-based visual imagery is persuasive in eliciting responses that match the hedonic nature of the platform. Therefore, fostering a positive emotional connection to both the community and the brand can lead to increased loyalty. This research proposes a different perspective on the interactive social exchange that facilitates the establishment of social capital. Value cocreation engagement is not necessarily dependent on the extensiveness of information depth. Adopting an affective orientation in persuasion has shown efficacy in forming attitudes towards attitudinal objects, particularly the community and brand.