bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of Aegilops speltoides and wheat repetitive elements and development of S genome-specific FISH painting
Authors: Danilova Tatiana V. , Akhunova Alina R. , Cai Xiwen 
Published: January 2025

Aegilops speltoides (2 n = 2 x = 14, genome SS) is a wild relative of wheat and a donor of useful traits for wheat improvement. Several whole-genome studies compared genic regions of Aegilops from the Sitopsis section and wheat and found that Ae. speltoides is most closely related to the wheat B subgenome but is not its direct progenitor. The results showed that a B subgenome ancestor diverged from Ae. speltoides more than 4 MYA and either has not yet been discovered, or is extinct. To further explore the evolutionary relationship between wheat and Ae. speltoides and develop Ae. speltoides chromosome paints, we performed comparative analysis of repetitive fractions of the S genome and three subgenomes of hexaploid wheat. The low-coverage sequence data were analyzed with RepeatExplorer pipeline to annotate repeats and estimate their content. The LTR-retrotransposons comprised about 80% of repeats in Ae. speltoides and wheat datasets and about two-third of them were LTR/Ty3-Gypsy. Ae. speltoides had 1.5 times more LTR/Ty-Copia repeats and 1.5 times less DNA transposons than wheat subgenomes. Several S genome-specific dispersed repeats were found and annotated. Their sequences were used to develop S genome-specific paints for detecting Ae. speltoides chromatin in the wheat background using fluorescent in situ hybridization.
Investigating the differences in the optical properties and laser photoluminescence for chitosan films extracted from two different origins
Authors: Ghazy Ahmed R. 
Published: January 2025

Abstract
This work carefully examines the variations in chitosan films derived from shrimp and crab shells in terms of their optical characteristics and laser-induced photoluminescence. Chitosan was dissolved in acetic acid and then cast into films to create chitosan films. The functional properties of chitosan generated from crab and prawns were shown to differ in specific spectrum regions due to differences in hydrogen bonding, degree of deacetylation, and structural conformations, as indicated by the FTIR study. Using TD-DFT and TD-DFT/CASTEP simulations, computational studies were carried out to predict the chitosan samples' molecular structures and frequency characteristics. Measurements and analyses were done on the optical characteristics, such as absorbance, refractive index, dielectric constants, and optical conductivity. Chitosan from shrimp shells (chitosan 2) and crab shells (chitosan 1) differed significantly in terms of UV absorbance, refractive index, dielectric constant, and optical conductivity, according to the study. Chitosan 2 showed increased dielectric constant, refractive index, and UV absorbance, indicating higher optical conductivity and a larger capacity for electrical energy storage, making it a better fit for optoelectronic applications. Chitosan's optical and photoluminescence characteristics are greatly influenced by its place of origin. These results emphasize how crucial it is to choose the right source material for a given optical application, especially in domains, where precise control over UV absorption and refractive index is necessary.
High-energy non-thermal, laser-induced nano-fusion
Authors: Csernai László P. ,   for the NAPLIFE Collaboration
Published: January 2025

Abstract
This is an invited talk contribution to the Particles & Plasmas Symposium 2024, held between Jun 10 and 12, 2024, in the Hotel Hilton in the Royal Castle Hill. As laser induced fusion is monochromatic and can be linearly polarized in the NAnoPlasmonic Laser Induced Fusion Energy (NAPLIFE) project, we aim for keeping nonthermal configuration during all stages of fusion.
Longitudinal Viral Load Clustering for People With HIV Using Functional Principal Component Analysis
Authors: Ma Yunqing , Yang Xueying , Xiao Jiayang , Li Xiaoming , Olatosi Bankole , Zhang Jiajia 
Published: January 2025

Background: Longitudinal measures of viral load (VL) are critical in monitoring the HIV status. While multiple lab indicators exist for monitoring measures of VL, research on clustering historical/longitudinal VL measures is limited. Analyzing longitudinal VL patterns rather than aggregated measures offers deeper insights into HIV status. This study uses functional data clustering to classify longitudinal VL patterns and characterize each cluster by demographics, comorbidities, social behaviors, and CD4 count.Methods: Adult PWH diagnosed from 2005 to 2015 in South Carolina with a 5‐year minimum follow‐up were included. We compared functional principal component analysis (FPCA), K‐means, hierarchical clustering, and Gaussian mixture models for classification and found FPCA yielded the best results. ANOVA was used to compare VL characteristics, demographics, comorbidities, substance uses, and longitudinal CD4 count across clusters.Results: Results obtained from FPCA could best distinguish the characteristics and patterns into four clusters. A total of 5916 PWH were grouped into long‐term VS group (Cluster 1, 17.3%), short‐term VS group (Cluster 2, 29.8%), suboptimal VS group (Cluster 3, 28.3%), and viral failure group (Cluster 4, 24.9%). In the long‐term VS group with an average of 11‐year follow‐up, PWH displayed sustained VS (95.3%) and lower mean CD4 count (95.3%) than other clusters. The short‐term VS group had shorter follow‐up (6 years), more comorbidities (31.4%), and lower percentage of time with low CD4 count (79.9%). In suboptimal VS group, PWH were mostly under 30 years old (44.8%) and Black (77.2%), with relatively lower mean VL (92.9%) and lower VR history (18.4%). In the viral failure group, PWH had higher mean VL (40.6%) and lower mean CD4 count (34.7%).Discussion: The findings highlight the impact of continuous clustering in understanding the distinct viral profiles of PWH and emphasize the importance of tailored treatment and insights to target interventions for all PWH.
Three-Dimensional CT for Preprocedural Planning of PCI for Ostial Right Coronary Artery Lesions: A Randomized Controlled Pilot Trial
Authors: van den Buijs Deborah M.F. , Poels Ella M. , Willems Endry , Cottens Daan , Dotremont Kevin , De Leener Karen , Meekers Evelyne , Ferdinande Bert , Vrolix Mathias , Dens Joseph , Ameloot Koen 
Published: January 2025

BACKGROUND:
Geographic stent-ostium mismatch is an important predictor of target lesion failure after percutaneous coronary intervention of an aorto-ostial right coronary artery lesion. Optimal visualization of the aorto-ostial plane is crucial for precise stent implantation at the level of the ostium. This study investigates whether preprocedural 3-dimensional computed tomography (3DCT), with determination of the optimal viewing angle, would allow for more precise stent implantation and reduce procedure time, contrast, and radiation dose.

METHODS:
In this single-center, prospective, open-label, core-laboratory blinded trial, a total of 30 patients with an aorto-ostial right coronary artery lesion were randomly assigned to either percutaneous coronary intervention with a preprocedural 3DCT or angiography-guided percutaneous coronary intervention. The optimal working view angle was determined by 3DCT in the intervention group and by the operators’ discretion in the control group. The primary end point was the percentage of patients without geographic mismatch, as determined by intravascular ultrasound.

RESULTS:

3DCT-determined C-arm angles were heterogenous but, in general, more extreme left anterior oblique projections were used (
P
<0.0001). While stent implantation was in the optimal position in all patients randomized to the intervention group, geographic mismatch was present in 5 (33%) patients randomized to the control group (
P
=0.06). The mean amount of procedural contrast (
P
<0.0001), mean radiation (
P
=0.03), and median procedure time (
P
=0.03) were significantly lower in the intervention group. The 3DCT scan was able to predict the calcium arc (
P
<0.0001) and minimal lumen area by intravascular ultrasound (
P
=0.003).

CONCLUSIONS:
Preprocedural 3DCT planning for percutaneous coronary intervention of aorto-ostial right coronary artery lesions allows for optimal stent positioning while reducing procedure time, contrast, and radiation dose.

REGISTRATION:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT05172323.
Enlightenment in the Dark Forest: Chan/Zen in Cixin Liu’s Three Body Trilogy
Authors: Van Overmeire Ben 
Published: January 2025

Abstract
This article explores the Chan/Zen Buddhist elements in Cixin Liu’s Three Body science fiction trilogy, particularly through the character of Luo Ji 羅輯. By exploring these references, the provocative moral and political philosophy articulated in the books can be better understood. Ultimately, I argue that Liu presents a modified version of Chan/Zen as the philosophy best fit for confronting a hostile universe.
Dialogical Aperture – Martin Buber’s Philosophy of Dialogue and Lu Nan’s Photography
Authors: Zhang Ping 
Published: January 2025

Abstract
This paper explores Martin Buber’s dialogical philosophy and its influence on Lu Nan’s 呂楠 photography. By comparing Lu Nan’s trilogy, which documents marginalized communities in China, with Tyagan Miller’s Covenant: Scenes from an African American Church, the study examines how Buber’s I-Thou concept shapes their practices. It highlights their efforts to transcend the subjectivity-objectivity divide in photography, as suggested by Susan Sontag. Through the “Dialogical Aperture” framework, this research provides insights into the ethical and aesthetic dimensions of documentary photography, proposing Buber’s philosophy as a pathway to more authentic representations of reality.
A Philosophy of Constancy in Change: Reading Gadamer in Light of Fundamental Themes in Chung-Ying Cheng
Authors: Fuyarchuk Andrew 
Published: January 2025

Abstract
The Western tradition of metaphysics has been criticized by Chung-ying Cheng on the grounds that it does not have any fruitful bearing on practice. This judgement, however, depends on a concept of metaphysics that Gadamer overturns with a Pythagorean-Platonic ontology. When this side of his philosophy is developed in tandem with Cheng’s onto-generative hermeneutics, in particular its doctrine of harmonization, new possibilities for self-understanding in relation to the grounds of existence are charted: The event of Being emerges from the reciprocity of rhythm in a dialogue self-similar to the rhythms of nature grasped by Cheng in terms of yin and yang. This consolidates a cross-fertilization between Cheng and Gadamer on the trans-historical foundations of hermeneutics.
Curating Queer Feminist Cinema: A Conversation Between Selina Robertson and James S. Williams
Authors: Robertson Selina , Williams James S. 
Published: January 2025

Abstract
“Curating Queer Feminist Cinema” examines the relations between the local and the transnational by considering the history and politics of queer feminist film curation in London. It takes the form of a conversation between the editor of the Special Issue, James S. Williams, and British queer feminist film programmer and curator Selina Robertson, who co-founded Club des Femmes, a London-based queer feminist curating collective. The discussion considers archiving queer history in cinema, feminism, queer and trans community, and film curatorial practice as research and a form of cultural and collective memory.
Mixed Layer Depth Measurement in Coastal Waters Utilizing Atmospheric Muons
Authors: Korkmaz M. Akif , Demirköz M. Bilge 
Published: January 2025

Abstract
Mixed layer (ML) in oceans is defined as the less dense upper region of the water column where turbulent mixing occurs and water exhibits almost uniform density profile. Mixed layer depth (MLD) is the depth of this region and shows diurnal and seasonal fluctuations as well as spatial variations in different regions of water. The determination of MLD is an important indicator for climate change in bodies of water, and the novel method proposed here would allow for a continuous monitoring of MLD. Atmospheric muon count is proportional to the density of water which can be measured by counting muons at the bottom and comparing with a shore-based or buoy-based muon counter. Combining this measurement with sea surface temperature (SST) and sea surface salinity (SSS) data from Earth-observing satellites, daily mean MLD can be estimated with an accuracy of 3% for down to 60-m depth. To determine MLD from muon count difference between surface and bottom detectors, a new method was introduced to calculate MLD using average density of water column. A Geant4 model was made to show the validity and limitations of the method. Unlike open ocean, the system can measure MLD in shallow coastal waters where muon count at the bottom is enough for daily based measurements. The proposed method can provide continuous in situ measurement of MLD in coastal shallow waters, and these data can be used to provide boundary conditions for hydrodynamic and ecosystem models.

Significance Statement
In our work, we proposed a scintillator-based underwater muon detection system which can measure average water column density by counting surviving atmospheric muons at the bottom of the shallow coastal waters. Combining this measurement with the sea surface temperature, salinity, and altimetry data from Earth-observing satellites, MLD can be estimated. In accordance with UN-SDG-14: Life Below Water, the determination of MLD is an important indicator for climate change in bodies of water, and the novel method proposed here would allow for a continuous monitoring of MLD.
Metronidazole is a chromatographic interference on a routine HPLC-UV assay for thiopurine metabolites
Authors: Wood Andrew A , Mason Sophie Rothwell , Robson Anna F 
Published: January 2025

Background Thiopurine metabolites, 6-thioguanine (6TG) and 6-methylmercaptopurine (6MMP), are monitored to aid therapeutic management of thiopurine drugs. At Manchester University NHS Foundation Trust (MFT), thiopurine metabolites are measured by high performance liquid chromatography with ultraviolet detection (HPLC-UV). Whole blood samples are lysed and subjected to hydrolysis with derivatisation of 6MMP before HPLC-UV detection at 304 nm for the 6MMP-derivative and 342 nm for 6TG. For some samples, 6MMP cannot be reported due to a chromatographic interference at 304 nm co-eluting with the 6MMP peak. An investigation was performed to identify the interfering compound. Methods Patient medication histories were examined to identify candidate compounds for the interference. Candidate compounds were spiked into blood at supraphysiological concentrations and tested on the assay. Results Metronidazole was identified as being prescribed to all patients whose samples demonstrated the interference. Metronidazole and its metabolite, hydroxymetronidazole, were spiked into blood. HPLC-UV analysis of spiked blood demonstrated similar UV absorbance patterns to those seen in patient samples with the interference. Hydroxymetronidazole co-eluted with 6MMP causing interference in the measurement. Conclusion Metronidazole and its major metabolite can interfere with 6MMP measurement by HPLC-UV analysis at 304 nm.
Do Competitive Markets Clean Up the US Electricity Sector?: Evidence From the Southwest Power Pool
Authors: Park Doyoung , Kaffine Daniel T. 
Published: January 2025

In the US, there has been a movement toward competitive electricity markets with system-operator auctions. However, the impact of such market reforms on non-market outcomes like emission rates remains unclear. We decompose how competition in wholesale electricity markets can affect carbon emission rates of electricity generating units (EGUs) through process, reallocation, and selection effects. Using event-study and difference-indifference strategies, we quantify the Day-Ahead Market’s (DAM) environmental impact in the Southwest Power Pool and assess the importance of each channel, with Pennsylvania, New Jersey, and Maryland interconnection serving as the comparison group. We find DAM reduced average carbon emission rates of EGUs by 0.033 tons per MWh, a roughly 4 percent reduction. This reduction is primarily driven by the selection effect: emission-intensive and uneconomical EGUs retire after the DAM market reform. This corresponds to an annual avoidance of 7.66 million tons of carbon dioxide emissions, worth approximately $383.4 million in avoided damages. JEL Classification: L51, L94, Q48, Q53
Real-world outcomes of Zilver Vena® Venous Self Expanding Stent placement for thrombotic and non-thrombotic indications in Spain
Authors: Ramirez Ortega Marta , Tejero Olivia Toledo , Benages Esteban Malo , Griggs Amy , Segbefia Edem , Mallagray Enrique Puras 
Published: January 2025

Purpose To evaluate longer term outcomes of the Zilver Vena Venous Stent in patients undergoing venous stenting. Materials and Methods Patients with iliofemoral obstructive venous disease and treated with venous stents were retrospectively enrolled in a physician-led real-world data collection effort. Results were analyzed by etiologies: post-thrombotic syndrome (PTS), non-thrombotic iliac vein lesion (NIVL), and iliocaval acute deep vein thrombosis (aDVT). Patency outcomes (primary, assisted-primary, secondary), reinterventions (in-stent and all), adverse events, and venous clinical outcome measures (VCSS, CEAP, Villalta Score) were reported using Kaplan-Meier estimates and summary statistics. Results A total of 219 patients (89.5% women, mean age 45.3 ± 11.9 years) were identified: 56 PTS, 153 NIVL, and 10 aDVT patients. Devices were placed across the inguinal ligament in 80.4%, 0%, and 70.0% of patients in the PTS, NIVL, and aDVT groups, respectively. Through 1-year, Kaplan-Meier estimated primary patency rate was 96.4% for PTS, 99.3% for NIVL, and 100% for aDVT patients, respectively, and followed the same trend through 3 years. Freedom from (FF) all and within stent reinterventions estimated by Kaplan-Meier was 87.9% and 90.1% for PTS, 98.2% and 98.2% for NIVL through 3 years. No reinterventions were reported for the aDVT group. Improved venous clinical outcome measures were seen in all groups at the last follow-up visit. Stent fractures occurred in 2 PTS patients without related reintervention or clinical sequelae. No stent migrations were reported. Conclusion Real-world use showed high patency rates and improved venous clinical outcome measures (VCSS, Villalta, and CEAP) after venous stent placement.
Multiple microcysts and clivus invasion diagnose T-box pituitary transcription factor 19 lineage adenomas in non-functioning pituitary adenomas
Authors: Zhao Xuening , Wang Xiaochen , Wang Sihui , Chen Lingxu , Yuan Mengyuan , Sun Shengjun 
Published: January 2025

Background Preoperative identification of T-box pituitary transcription factor 19 (TPIT) lineage silent adenomas in non-functioning pituitary adenomas (NFPAs) is important. Purpose To compare the clinical, laboratory, and radiological features of the three cell lineages of adenomas in NFPAs and evaluate the diagnostic efficacy of multiple microcysts and clivus invasion on magnetic resonance imaging (MRI) for TPIT lineage adenomas in NFPAs. Material and Methods A total of 405 patients with NFPA were retrospectively enrolled, including steroidogenic factor 1 (SF-1) lineage adenomas (n = 204), TPIT lineage adenomas (n = 111), and pituitary transcription factor 1 (PIT-1) lineage adenomas (n = 90). The clinical, laboratory, and radiological features of the three lineages adenomas were compared. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of multiple microcysts, clivus invasion, and their combination were calculated to diagnose TPIT lineage adenomas in NFPAs. Results Among the three lineages of NFPAs, patients with SF-1 lineage were older than those with TPIT and PIT-1 lineages ( P < 0.001). TPIT lineage adenomas were most common in women ( P < 0.001) and had the highest tumor volume ( P < 0.001), and incidence of clivus invasion ( P < 0.001). The multiple microcysts and clivus invasion in the diagnosis of TPIT lineage adenomas in NFPAs had high specificity (88.44% vs. 98.64%) and accuracy (77.28%). Conclusion The MRI findings of multiple microcysts and clivus invasion can help diagnose TPIT lineage adenomas in NFPAs with high specificity.
Aesthetic Perception to Different Facial Profiles—An Evaluation Based on Age, Sex, Educational Status, and Study Area
Authors: Sada Maria Gabriela Peraldi , Garcia Luis Alberto Gonzalez , Martinez Marco Antonio Martinez 
Published: January 2025

Objectives This study aimed to determine the aesthetic preferences of profile silhouettes according to various soft tissue analyses commonly used in orthodontic practice. Methods For this observational study, lateral photographs were taken of two patients. Nasolabial and mentolabial angles were digitally altered in 20° increments, obtaining four photographic series, and a survey was carried out, recruiting 330 participants to evaluate their preferences according to these categories: very attractive, somewhat attractive, little attractive, and unattractive. The relationship between variables was evaluated using the chi-square test ( χ2) and Cohen’s d ( d) for effect size. Results Significant correlations were obtained between variables ( p < .001; p < .05). Female participants, those 23-33 years old, graduates, and those from Health Sciences obtained a higher prevalence when choosing the most balanced facial profile. Differences in the nasolabial angle were more detected. Conclusion There was a tendency to prefer profiles with a more open nasolabial angle and a flatter mentolabial sulcus in women. The variables that proved to have the most influence were educational status and age.
Prepubertal phthalate exposure can cause histopathological alterations, DNA methylation and histone acetylation changes in rat brain
Authors: Koc Seyda , Erdogmus Ekin , Bozdemir Ozlem , Ozkan-Vardar Deniz , Yaman Unzile , Erkekoglu Pınar , Zeybek Naciye Dilara , Kocer-Gumusel Belma 
Published: January 2025

Di-2-(ethylhexyl)phthalate (DEHP) is a phthalate derivative used extensively in a wide range of materials, such as medical devices, toys, cosmetics, and personal care products. Many mechanisms, including epigenetics, may be involved in the effects of phthalates on brain development. In this study, Sprague-Dawley male rats were obtained 21–23 days after their birth (post-weaning) and were exposed to DEHP during the prepubertal period with low-dose DEHP (DEHP-L, 30 mg/kg/day) and high-dose DEHP (DEHP-H, 60 mg/kg/day, 37 days) until the end of adolescence (PND 60). The rats in the study groups were sacrificed during adulthood, and histopathological changes, epigenetic changes, and oxidative stress parameters were evaluated in brain tissues. Histopathological findings indicating the presence of deterioration in brain tissue morphology were obtained, more prominently in the DEHP-H group. Examining the hippocampus under the light microscope, pyramidal neuron loss was detected only in CA1 of the DEHP-L group, while in DEHP-H rats, pyramidal neuron losses were detected in the CA1, CA2, and CA3 regions. No significant change was observed in brain lipid peroxidation levels with DEHP compared to control. Significant increases in total glutathione (GSH) in both dose groups were considered to be an adaptive response to DEHP-induced oxidative stress. The decrease in DNA methylation in the brain, although not statistically significant, and the increase in histone modification showed that exposure to DEHP may cause epigenetic changes in the brain and these epigenetic changes may also take place as one of the mechanisms underlying the damage observed in the brain. The results suggest that DEHP exposure during early development may have a significant effect on brain development.