bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Clinical Success and Molecular Mechanisms of Action of Novel Anti-glioblastoma Drugs: A Review
Authors: Abdullaeva Sabina , Chubarev Vladimir , Valeeva Anna , Preferanskaya Nina , Neganova Margarita , Smolyarchuk Elena , Liu Junqi , Sukocheva Olga , Samsonov Mikhail , Alyautdin Renad 
Published: February 2024

Background:
Gliomas and glioblastomas (GBM) are common primary malignant brain tumors,
which are highly malignant and have a poor prognosis. The presence of cancer stem cells with unrestricted
proliferative capacity and ability to generate glial neoplastic cells, the diffuse nature of GBM,
and other specific factors of GBM contribute to poor results of drug therapy in patients with GBM. Despite
the worldwide efforts to improve the treatment, many novel anti-GBM drugs are active just in
vitro, in silico, and in preclinical trials, and they sometimes demonstrate poor or no activity in clinical
trials. In this paper, we have casually selected and analyzed the most promising evidence-based results
related to glioblastoma treatment at FDA and Clinical Trials.gov databases. It was observed that the
most prospective trend in the development of anti-GBM drugs is combination therapy vs.. monotherapy.
Our analysis of clinical trials has allowed us to predict that the most promising combination therapy that
has shown the best results in patient’s surveillance should include drugs that block different growth-promoting
signals in glioblastoma cells and that are activated by the V600E BRAF mutation. One drug
should inhibit signals from the BRAF protein, whereas the second drug in combination should inhibit
signals from the MEK protein

Methods:
The content of this review is based on information obtained from PubMed, ClinicalTrials.-
gov, and the U.S. Food and Drug Administration (https://www.fda.gov/). In ClinicalTrials.gov, we retrieved
studies published from January 1, 2015. In the data search, “Glioblastoma” was used as the keyword.
A study was deleted if it studied remedies for concomitant tumor diseases, as well as if it did not
include descriptions of treatment methods and/or if GBM was not mentioned. The analysis of the effectiveness
of treatment was carried out according to the increasing overall survival in GBM patients, compared
to the gold standard for this cancer.

Results:
GBM patients treated with novel immunotherapy agents and drugs acting on epigenetic factors
and receptor tyrosine kinase inhibitors have shown encouraging potential for future development in clinic.
However, combinations of drugs have led to more significant improvements in the results and an increase
in life expectancy of patients. For example, the combination of nivolumab and ipilimumab
showed a 72% increase in life expectancy compared to using nivolumab alone (9.8 vs.. 16.85).

Conclusion:
Combining anti-GBM drugs appears to be a key direction for increasing treatment effectiveness
and overall survival. Radiotherapy of GBM can increase the effect of combination drug therapy.
3D Printing of Microfluidic-assisted Liposomes Production for Drug Delivery and Nanobiomedicine: A Review
Authors: Mohammad-Jafari Kave , Naghib Seyed Morteza 
Published: February 2024

Abstract:
Numerous techniques exist for the production of liposomes; however, these
methods need to be revised due to their incapacity to achieve precise management of the
dimensions and uniformity of liposomes and their inefficient utilization of reagents and
resources. One particular challenge lies in replicating accurate form and size control
seen in biological cells, as accomplishing this level of precision through macroscale approaches
proves exceptionally arduous. The advent of microfluidic technology tackles
this problem by lowering liposome synthesis to a centimeter-level chip, drastically cutting
related costs, and enhancing liposome manufacturing efficiency and mobility. Although
various microfluidic technologies for micro or nanoparticle preparation have
been established, manufacturing microfluidic devices poses challenges due to their high
cost and time-consuming nature. However, a promising and cost-effective solution lies
in additive production, commonly guided by 3D printing. This innovative technique has
demonstrated significant potential and has been successfully applied to create microfluidic
chips. Here, we will explore using 3D printing to produce microfluidic devices
specifically designed for liposome production. Moreover, the biomedical applications
of the liposomes produced by 3D printing-fabricated chips will be fully discussed.
Investigating the Effect of Vitamin A on Orthodontic Tooth Movement: An Experimental Study
Authors: Shi Pengxiang , Zhao Jing , Wang Feng 
Published: February 2024

Background:
Vitamin A is essential not only for bone metabolism and development
but also for the normal functioning of many physiological processes in the body. Despite vitamin
A's important involvement in bone metabolism, its effect on orthodontic tooth movement is not
entirely known.

Aim:
Previous studies on animals have suggested that vitamin A may influence alveolar bone
remodelling and tooth movement, but the effect of various doses of vitamin A on these processes
remains poorly understood. This experiment was designed to examine the effect of vitamin A on
the orthodontic tooth movement of male rats.

Methods:
Eighty male rats weighing 200-250 grams were divided into eight equal parallel
groups. An initial orthodontic force was applied to all groups with a specific appliance, and six
different doses of vitamin A were administered (250-2500 IU/Kg intraperitoneally). Two control
groups were also considered. Orthodontic tooth movement was measured at the beginning and
end of the study period (day 14), and serum alkaline phosphatase (ALP) level was evaluated. The
maxillary sections were also evaluated by histological examination.

Results:
Although there was a dose-dependent increase in tooth movement observed with vitamin
A administration, the differences were not statistically significant. There was no significant
difference in the number of osteoclasts or the presence of lacunae on the root surface between
the study groups. Root resorption was observed in different areas of the root and was not related
to different doses of vitamin A. The serum ALP level did not show any significant difference between
the groups treated with different doses of vitamin A.

Conclusion:
Despite the known effects of vitamin A on bone metabolism, the results of this
study suggest that vitamin A did not increase alveolar bone remodeling and orthodontic tooth
movement in male rats.
Understanding the Molecular Mechanisms of H2's Regulatory Effect on miR-29s for Brain Protection during Deep Hypothermic Circulatory Arrest
Authors: Li Hongmei , Shen Li , Ge Junwen , Li Hongyun , Chen Feng , Chen Xiaolong , Hong Haifa 
Published: February 2024

Introduction:
Research regarding post-operative brain protection after deep hypothermic
circulatory arrest (DHCA) has gained attracted significant attention. We previously
demonstrated that hydrogen can significantly reverse DHCA-induced brain damage.

Method:
In the current research, we have established the DHCA model successfully using a
modified four-vessel occlusion method and injected miR-29s compounds into the hippocampal
tissue of rats.

Result:
We were surprised to find hydrogen increased miR-29s expression in the hippocampal
tissue of a DHCA rat model. The administration of agomiR-29s counteracted DHCA-induced
hippocampal tissue injury, while the antamiR-29s had the opposite effects.

Conclusion:
Based on the above facts, the brain protection mechanism of hydrogen in DHCAtreated
rats may be related to the upregulation of miR-29s, which can exert its beneficial effects
by alleviating apoptosis, inflammation, and oxidation.
Emerging Phytochemical Formulations for Management of Rheumatoid Arthritis: A Review
Authors: Pimple Prachi , Shah Jenny , Singh Prabha 
Published: February 2024

:
Rheumatoid arthritis (RA) is a T-cell-mediated chronic inflammatory disorder affecting
0.5-1% of the global population. The disease with unknown etiology causes slow destruction of
joints, advancing to significant deterioration of an individual’s quality of life. The present treatment
strategy comprises the use of disease-modifying anti-rheumatic drugs (DMARDs) coupled with or
without nonsteroidal anti-inflammatory drugs or glucocorticoids. Additionally, involves co-therapy
of injectable biological DMARDs in case of persistent or recurrent arthritis. The availability of biological
DMARDs and the implementation of the treat-to-target approach have significantly improved
the outcomes for patients suffering from RA. Nevertheless, RA requires continuous attention due to
inadequate response of patients, development of tolerance and severe side effects associated with
long-term use of available treatment regimens. An estimated 60-90% of patients use alternative
methods of treatment, such as herbal therapies, for the management of RA symptoms. Over the past
few decades, researchers have exploring natural phytochemicals to alleviate RA and associated
symptoms. Enormous plant-origin phytochemicals such as alkaloids, flavonoids, steroids, terpenoids
and polyphenols have shown anti-inflammatory and immunomodulatory activity against RA. However,
phytochemicals have certain limitations, such as high molecular weight, poor water solubility,
poor permeability, poor stability and extensive first-pass metabolism, limiting absorption and bioavailability.
The use of nanotechnology has aided to extensively improve the pharmacokinetic profile
and stability of encapsulated drugs. The current review provides detailed information on the therapeutic
potential of phytochemicals. Furthermore, the review focuses on developed phytochemical
formulations for RA, with emphasis on clinical trials, regulatory aspects, present challenges, and
future prospects.
Nanotechnology-based Herbal Drug Formulation in the Treatment of Diabetes Mellitus
Authors: Bhadouria Namrata , Alam Aftab , Kaur Awaneet 
Published: February 2024

:
The utilization of nanotechnology-based herbal medication delivery systems is gaining
attention as a novel approach to treating diabetes mellitus. The incorporation of nanotechnology
into herbal medicine provides benefits such as enhanced Stability, solubility, and bioavailability
of herbal medications. The purpose of this paper is to summarise the present status of research
on herbal medicine delivery systems based on nanotechnology for the treatment of diabetic
patients. The paper evaluates the various nanocarriers and herbal drugs used, the challenges
and opportunities in the development of these systems, and their potential efficacy and safety.
Additionally, the paper highlights the need for further research to optimize the formulation and
delivery of these systems. This review's overarching objective is to provide a complete understanding
of the possibilities of herbal medication delivery systems based on nanotechnology in
diabetes mellitus treatment.
The Role of Prebiotic, Probiotic, and Synbiotic in Gut Microbiota and Gut
Permeability in Children Affected by Air Pollution
Authors: Darma Andy , Dewi Dian Kusuma , Chandra Dian Novita , Basrowi Ray Wagiu , Khoe Levina Chandra , Pratiwi Dessy , Sundjaya Tonny 
Published: February 2024

Background:
Air pollution has been linked with gut microbiota dysbiosis. Ingested environmental pollutants may alter gut microbiota compositions by changing the environment of the
gut. Gut microbiota dysbiosis has been observed in children with asthma, linking the possible role
of gut microbiota with systemic immune response and asthma.

Methods:
This paper aims to identify current science on how prebiotics, probiotics, and synbiotics
can improve gut microbiota dysbiosis.

Results:
We reviewed the existing literature related to the role of pre-, pro-, and synbiotics in
child health, and the evidence mapping method was chosen as the rapid review to identify gaps in
knowledge and future research needs.

Conclusion:
In conclusion, the current evidence on the role of prebiotics, probiotics, and synbiotics on child health, while limited, showed promising results on the allergy and immunology pathway, including infection prevention for the gastrointestinal and respiratory tract.
Impact of Clinical Pharmacist-conducted Medication Reconciliation at Admission and Discharge on Medication Safety in Patients Hospitalized with Acute Decompensated Heart Failure
Authors: Rangchian Maryam , Makhdoumi Mana , Zamanirafe Maryam , Parvaneh Erfan , Eshraghi Azadeh , Entezari-Maleki Taher , Mehrpooya Maryam 
Published: February 2024

Background:
Most studies have focused on the impact of medication reconciliation
on one of the points of hospital admission or discharge. In this study, we aimed to investigate the
impact of medication reconciliation on medication safety in patients hospitalized with acute decompensated
heart failure at both admission and discharge.

Methods:
This was a prospective, single-center, cohort study conducted in a tertiary care cardiovascular
hospital from December 2022 to May 2023 on patients hospitalized with acute decompensated
heart failure. Patients were considered eligible if they were taking at least five chronic medications
prior to hospital admission. Medication reconciliation was carried out for the study patients
by a clinical pharmacy team both at admission and discharge. Additionally, the study patients also
received comprehensive discharge counseling as well as post-discharge follow-up and monitoring.

Results:
Medication reconciliation was applied for 129 patients at admission and 118 at discharge.
The mean time needed for medication reconciliation presses was 32 min per patient at admission
and 22 min at discharge. Unintentional medication discrepancies were relatively common at both
admission and discharge, but compared to admission, discrepancies were less frequent at discharge
(178 versus 72). Based on the consensus review, about 30% of identified errors detected at both
admission and discharge were judged to have the potential to cause moderate to severe harm to the
patient. Most of the clinical pharmacists’ recommendations on unintended discrepancies were accepted
by physicians and resulted in changes in medication orders (more than 80%). Further, the
majority of the participants were ‘very satisfied’ or ‘satisfied’ with the clinical pharmacy services
provided to them during hospitalization and after hospital discharge (89.90%).

Conclusion:
Our results demonstrated the vulnerability of heart failure patients to medication discrepancies
at both admission and discharge. Thus, implementing a comprehensive medication reconciliation
by clinical pharmacists could be beneficial for enhancing medication safety in these patients.
Genetic Polymorphisms of GP1BA, PEAR1, and PAI-1 may be Associated
with Serum sIgE and Blood Eosinophil Levels in Chinese Patients with
Allergic Diseases
Authors: Tang Rui , Lyu Xiaohong , Sun Jinlyu , Li Hong 
Published: February 2024

Background:
It has been suggested that genetic factors may be substantially linked to
allergy disorders.

Objective:
This study aims to investigate the relationship between the serum specific Immunoglobulin
E (sIgE), blood eosinophil, and the polymorphisms of glycoprotein Ib alpha gene
(GP1BA) rs6065, platelet endothelial aggregation receptor 1 gene (PEAR1) rs12041331, and
plasminogen activator inhibitor 1 gene (PAI-1) rs1799762.

Methods:
From the Peking Union Medical College Hospital, this study enrolled 60 healthy participants
and 283 participants with allergic diseases. TaqMan-minor groove binder (MGB)
quantitative polymerase chain reaction (qPCR) was used to examine the gene polymorphisms in
each group.

Results:
The TaqMan-MGB qPCR results were completely consistent with the DNA sequencing
results, according to other studies in this medical center (Kappa =1, p < 0.001). The GP1BA
rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms did not show different distribution
between allergy patients and healthy individuals. Concerning allergy patients, the CT
(n=33) genotype of GP1BA rs6065 had higher blood eosinophil level than the CC (n=250)
genotype (0.59, IQR 0.32-0.72 vs 0.31, IQR 0.15-0.61, *109/L, p =0.005). The serum sIgE of
AA (n=46) genotype of PEAR1 rs12041331 was lower (median 3.7, interquartile quartiles
(IQR) 0.2-16.8, kU/L) than the GA (n=136) and GG (n=101) genotypes (GA median 16.3, IQR
3.1-46.3, kU/L, p = 0.002; GG median 12.9, IQR 3.0-46.9, kU/L, p =0.003). The GA genotypes
of PEAR1 rs12041331were with higher blood eosinophil levels (median 0.42, IQR 0.17-0.74
*109/L) than the AA genotype (median 0.25, IQR 0.15-0.41*109/L, p =0.012). The sIgE of the
5G5G (n=44) genotype of PAI-1 rs1799762 was lower (median 5.0, IQR 0.1-22.8, kU/L) than
the 4G5G (n=144) (median 17.3, IQR 3.7-46.0, kU/L, p = 0.012).

Conclusion:
The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms
may be associated with the genetic susceptibility of serum sIgE or blood eosinophil in Chinese
allergic disease patients.
Evaluation of Serum Levels of Vitamin D3 and IgE in Patients with Chronic and Allergic Sinusitis: A Cross-sectional Study
Authors: Bagheri Payman , Nouri Majid , Eskandarzadeh Hesam , Darvishi Mohammad 
Published: February 2024

Background:
Chronic rhinosinusitis is known as a common problem with inflammatory
and allergic causes. Several factors are associated with developing chronic rhinosinusitis,
including immunoglobulin E (IgE) production and vitamin D deficiency.

Objective:
In this study, we investigated the role of IgE and Vitamin D deficiency and differences
between patients with chronic, allergic sinusitis and controls.

Methods:
A total of 90 subjects were included in 3 groups (n=30) in this cross-sectional, correlational
descriptive study. The subjects were divided into three groups, including control
(healthy subjects), chronic sinusitis patients, and allergy patients. A checklist was used to collect
the necessary data, including age, gender, and body mass index (BMI). To evaluate serum
levels of vitamin D3 and IgE, ELISA kits were used.

Results:
The mean vitamin D was 22 g/ml. Fifty-four participants (60%) out of all included
people had insufficient vitamin D, 13% had a deficiency, and the high deficiency and insufficiency
were in the group of allergic sinusitis. Our results indicated that gender (female) was
significantly associated with vitamin D deficiency (p =0.01). Thirty-nine participants (43.3%)
out of all studied subjects had high IgE, and the highest level of abnormality of IgE was in the
allergic sinusitis group. Furthermore, it was found that gender and IgE were not significantly
related. However, IgE was significantly associated with vitamin D deficiency in the allergic sinusitis
group.

Conclusion:
Our findings highlighted that most of the patients with chronic and allergic sinusitis
had insufficient vitamin D. A possible association was also found between low vitamin D
and IgE levels and the prevalence of allergic sinusitis. This study showed that patients with allergic
sinusitis may be more vulnerable to lower serum levels of vitamin D. Therefore, vitamin
D supplementation as an adjunctive therapy may be considered in these patients.
Virtual Reality for Developing Patient-Facing Communication Skills in Medical and Graduate Education: Protocol for a Scoping Review
Authors: Khodabakhshian Nairy , Gaeul Lee Kyla , Marawi Tulip , Sorkhou Maryam , Vyravanathan Sobiga , Harnett Nicole 
Published: February 2024

Background
Clinician-patient communication is an integral component in providing quality medical care. However, research on clinician-patient communication has shown overall patient discontent with provider communication skills. While virtual reality (VR) is readily used for procedural-based learning in medical education, its potential for teaching patient-facing communication skills remains unexplored. This scoping review aims to evaluate the effectiveness and feasibility of VR applications used for patient-facing communication skills development in medical education.

Objective
The primary objective is to synthesize and evaluate the effectiveness of available VR tools and applications used for patient-facing communication skills development in medical education. The secondary objectives are to (1) assess the feasibility of adapting VR applications to develop patient-facing communication skills in medical education and (2) provide an overview of the challenges associated with adapting VR applications to develop patient-facing communication skills in medical education.

Methods
A total of 4 electronic databases (ERIC, Embase, PubMed, and MEDLINE) were searched for primary peer-reviewed articles published through April 11, 2023. Articles evaluating the implementation of non-, semi-, and fully immersive VR training for patient- or caregiver-facing communication skills training provided to graduate, medical, or other allied health care professions students were included. Studies that assessed augmented reality, mixed reality, artificial intelligence, or VR for non–communication-based training were excluded. Study selection will include a title, abstract, and full-text screening by 4 authors. Data from eligible studies will be extracted and entered into a database and presented in tabular format. Findings will be reported according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines for scoping reviews.

Results
As of April 11, 2023, the search strategy has been confirmed and the search has been completed. We are currently at the title and abstract screening stage. Once complete, the articles will undergo full-text screening according to eligibility criteria as described in the methods.

Conclusions
The findings of this review will inform the development of a graduate-level clinical skills research course within the Institute of Medical Science graduate department at the University of Toronto. It is also expected that these findings will be of interest to other health care–specific faculties inside and beyond our institution. Further, our scoping review will summarize the limited field of literature on VR use in medical communications training and identify areas for future inquiry.

International Registered Report Identifier (IRRID)
DERR1-10.2196/53901
Simultaneous Analysis of Dopamine and Ascorbic Acid Using Polymelamine/Gold Nanoparticle-Modified Carbon Paste Electrode
Authors: Harsini Muji , Farida Ainy Nur , Fitriany Erna , Paramita Denok Risky Ayu , Baktir Afaf , Kurniawan Fredy , Sakti Satya Candra Wibawa , Kurniawan Yudhi Dwi , Widyaningrum Bernadeta Ayu 
Published: February 2024

Modification of electrode using polymelamine (PM) and gold nanoparticles (AuNPs) has been successfully developed via electropolymerization and electrodeposition onto carbon paste electrode (CPE) using cyclic voltammetry (CV) technique. The modified electrode (AuNPs/PM/CPE) was applied as voltammetry sensors in a simultaneous of dopamine (DA) and ascorbic acid (AA). AuNPs/PM/CPE presented an effective surface area 5 times wider than CPE and demonstrated good electrocatalytic performance in the oxidation of DA and AA in 0.1 M phosphate buffer solution (pH 3) with a scan rate of 100 mV s−1. The differential pulse voltammetry (DPV) technique was chosen as the best method for separating potential peaks of DA and AA. The linear response for determining DA and AA using the DPV technique produced a concentration range of 0.1–13 and 0.4–12 µM with coefficient linearity of 0.9999 and 0.9997, the limit of detection of 0.1405 and 0.2187 µM, the accuracy of 89.62–109.16%, and 83.63–105.08%, and the precision of 0.017–0.701% and 0.066–0.626%, respectively. In addition, this electrode was applied in a real sample of infant urine with a concentration of 1 µM by spike method and found 98.86 and 98.28% as percent recovery of DA and AA, respectively.