bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety of Bioplasma FDP and Hemopure in rhesus macaques after 30% hemorrhage
Authors: Pusateri Anthony E , Morgan Clifford G , Neidert Leslie E , Tiller Michael M , Glaser Jacob J , Weiskopf Richard B , Ebrahim Ismaeel , Stassen Willem , Rambharose Sanjeev , Mahoney Scott H , Wallis Lee A , Hollis Ewell M , Delong Gerald T , Cardin Sylvain 
Published: January 2024

ObjectivesPrehospital transfusion can be life-saving when transport is delayed but conventional plasma, red cells, and whole blood are often unavailable out of hospital. Shelf-stable products are needed as a temporary bridge to in-hospital transfusion. Bioplasma FDP (freeze-dried plasma) and Hemopure (hemoglobin-based oxygen carrier; HBOC) are products with potential for prehospital use. In vivo use of these products together has not been reported. This study assessed the safety of intravenous administration of HBOC+FDP, relative to normal saline (NS), in rhesus macaques (RM).MethodsAfter 30% blood volume removal and 30 minutes in shock, animals were resuscitated with either NS or two units (RM size adjusted) each of HBOC+FDP during 60 minutes. Sequential blood samples were collected. After neurological assessment, animals were killed at 24 hours and tissues collected for histopathology.ResultsDue to a shortage of RM during the COVID-19 pandemic, the study was stopped after nine animals (HBOC+FDP, seven; NS, two). All animals displayed physiologic and tissue changes consistent with hemorrhagic shock and recovered normally. There was no pattern of cardiovascular, blood gas, metabolic, coagulation, histologic, or neurological changes suggestive of risk associated with HBOC+FDP.ConclusionThere was no evidence of harm associated with the combined use of Hemopure and Bioplasma FDP. No differences were noted between groups in safety-related cardiovascular, pulmonary, renal or other organ or metabolic parameters. Hemostasis and thrombosis-related parameters were consistent with expected responses to hemorrhagic shock and did not differ between groups. All animals survived normally with intact neurological function.Level of evidenceNot applicable.
Another piece of the hemolytic disease of the fetus and newborn puzzle after RhD-positive transfusion in trauma resuscitation: the proportion of pregnant women who produce high titer anti-D
Authors: Yazer Mark H , Emery Stephen P , Triulzi Darrell J , Spinella Philip , Leeper Christine 
Published: January 2024

BackgroundAfter the transfusion of RhD-positive red blood cell (RBC)-containing products to an RhD-negative woman of childbearing potential (WCP) during trauma resuscitation, there are several events that must occur for that WCP to have a future pregnancy affected by hemolytic disease of the fetus and newborn (HDFN). This study identified and quantitated the frequency of a novel event in the sequence from RhD-positive transfusion during trauma resuscitation to an HDFN outcome, that is, the development of a high titer anti-D among women who were D-alloimmunized.MethodsThe transfusion service records at one maternity hospital were searched to locate all anti-D titers that had been performed on pregnant women between 1996 and 2022. The highest titer score during each pregnancy was recorded for this study. The critical titer threshold at this institution was ≥16. Passive anti-D caused by Rh immunoglobulin were excluded from analysis.ResultsThere were 97 pregnancies in 85 patients who had an immune-stimulated anti-D; in 60 of 97 (62%) pregnancies, the highest titer score was ≥16. There were 12 patients who had titers performed in two pregnancies during the study period; the correlation between the maximum titer in each pregnancy was not statistically significant (Spearman rank correlation r=0.42, p=0.17).ConclusionIn this single center study, 62% of D-alloimmunized pregnant women had a high titer antibody. When considering all of the events that must occur for HDFN to happen, the rate of perinatal mortality was calculated to be 0.04% and the rate of perinatal death or serious adverse event from HDFN was 0.24%.
A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing
Authors: Wang Chien-Wei , Weaver Simon D. , Boonpattrawong Nicha , Schuster-Little Naviya , Patankar Manish , Whelan Rebecca J. 
Published: January 2024

Abstract

The biomarker CA125, a peptide epitope located in several tandem repeats of the mucin MUC16, is the gold standard for monitoring regression and recurrence of high-grade serous ovarian cancer in response to therapy. However, the CA125 epitope along with several structural features of the MUC16 molecule are ill defined. One central aspect still unresolved is the number of tandem repeats in MUC16 and how many of these repeats contain the CA125 epitope. Studies from the early 2000s assembled short DNA reads to estimate that MUC16 contained 63 repeats.
Here, we conduct Nanopore long-read sequencing of MUC16 transcripts from three primary ovarian tumors and established cell lines (OVCAR3, OVCAR5, and Kuramochi) for a more exhaustive and accurate estimation and sequencing of the MUC16 tandem repeats.
The consensus sequence derived from these six sources was confirmed by proteomics validation and agrees with recent additions to the NCBI database. We propose a model of MUC16 containing 19—not 63—tandem repeats. In addition, we predict the structure of the tandem repeat domain using the deep learning algorithm, AlphaFold.
The predicted structure displays an SEA domain and unstructured linker region rich in proline, serine, and threonine residues in all 19 tandem repeats. These studies now pave the way for a detailed characterization of the CA125 epitope. Sequencing and modeling of the MUC16 tandem repeats along with their glycoproteomic characterization, currently underway in our laboratories, will help identify novel epitopes in the MUC16 molecule that improve on the sensitivity and clinical utility of the current CA125 assay.

Significance:
Despite its crucial role in clinical management of ovarian cancer, the exact molecular sequence and structure of the biomarker, CA125, are not defined. Here, we combine long-read sequencing, mass spectrometry, and in silico modeling to provide the foundational dataset for a more complete characterization of the CA125 epitope.
MEBRAINS 1.0: a new population-based macaque atlas
Authors: Balan Puiu F , Zhu Qi , Li Xiaolian , Niu Meiqi , Rapan Lucija , Funck Thomas , Bakker Rembrandt , Palomero-Gallagher Nicola , Vanduffel Wim 
Published: January 2024

Abstract
Due to their fundamental relevance, the number of anatomical macaque brain templates is constantly growing. Novel templates aim to alleviate limitations of previously published atlases and offer the foundation to integrate multiscale multimodal data. Typical limitations of existing templates include their reliance on one subject, their unimodality (usually only T1 or histological images), or lack of anatomical details. The MEBRAINS template overcomes these limitations by using a combination of T1 and T2 images, from the same 10 animals (Macaca mulatta), which are averaged by the multi-brain toolbox for diffeomorphic registration and segmentation. The resulting volumetric T1 and T2 templates are supplemented with high quality white and gray matter surfaces built with FreeSurfer. Human-curated segmentations of pial surface, white/gray matter interface and major subcortical nuclei were used to analyse the relative quality of the MEBRAINS template. Additionally, 9 CT scans of the same monkeys were registered to the T1 modality and co-registered to the template. Through its main features (multi-subject, multi-modal, volume-and-surface, traditional and deep learning-based segmentations), MEBRAINS aims to improve integration of multi-modal multi-scale macaque data and is quantitatively equal to, or better than, currently widely used macaque templates. We provide a detailed description of the algorithms/methods used to create the template aiming to furnish future researchers with a map-like perspective which should facilitate identification of an optimal pipeline for the task they have at hand. Finally, recently published 3D maps of the macaque inferior parietal lobe, (pre)motor and prefrontal cortex were warped to the MEBRAINS surface template, thus populating it with a parcellation scheme based on cyto- and receptor architectonic analyses. The template is integrated in the EBRAINS and Scalable Brain Atlas web-based infrastructures, each of which comes with its own suite of spatial registration tools.
The Role of the Colonial State in the Spread and Strengthening of Christianity in Colonial Tanganyika, Circa 1890–1961
Authors: Seimu Somo M.L. , Komba Yustina Samwel 
Published: January 2024

Abstract
This paper makes an essential contribution by arguing that shifts in German colonial educational policy, which continued under British control, consistently favoured the spread of Christianity over Islam in Tanganyika. The paper also looks at how the two colonial powers used Article 6 of the Berlin Act 1884–1885, Article 438 of the Versailles Treaty, and Article 22 of the League of Nations Covenant, as well as Command Paper No. 2374 of I925 that emphasised the protection of Christian missionaries and the provision of education by missionaries across British colonies in Africa. The aforementioned articles established colonial state-missionary partnerships and provided security to missionaries. The colonial authority’s protection of missionaries was essential to investing in the education sector, which became a centre for the spread of Christianity in the country. As a result, the spread of Christianity throughout the territory accelerated. The paper relies heavily on secondary and primary sources for this analysis.
Patronage, Medicine, and Piety in Ayyubid Damascus
Authors: Sánchez Ignacio 
Published: January 2024

Abstract
The first part of this article, published in 2021, discussed the status of medicine within Islamic societies and changing attitudes towards this discipline until the Ayyubid period that led to the so-called Islamisation of medicine. This second part will survey the sources conveying information about a unique phenomenon that took place in seventh/thirteenth-century Damascus: the endowment of madrasas for the study of medicine.
Eustachian Tube Balloon Dilation in Children: Short- and Long-Term Outcome
Authors: Gürtler Nicolas , Honegger Flurin 
Published: January 2024

Objective: Eustachian Tube Balloon Dilation (ETBD) represents an innovative therapeutic approach for chronic Eustachian tube dysfunction (CETD), a common disease in children. Some evidence of a benefit of ETBD in the adults exist in contrast to sparse reports in children. The objective was to analyze short- and long-term outcome of ETBD in children with CETD. Methods: A retrospective chart-review was performed in a cohort of 19 children (mean age 13 years, 7-17) who underwent ETBD. The following parameters were analyzed: tubomanometry ( R-value), tympanogram, hearing (CPT-AMA, Air-bone gap [ABG]), Eustachian Tube Score (ETS and ETS-7), and Eustachian Tube Disease questionnaire (ETDQ). Results: Twenty-four ears were dilated (in 5 patients subsequently after successful first intervention) and grouped as A (17) without and B (7) with additional T-tube insertion. Most children suffered from either chronic otitis media with effusion or chronic perforation (12, 63%), the remainder comprising recurrent otitis media, adhesive otitis media and CETD with barotrauma. Mean duration of symptoms were 7/8.2 years and mean follow-up 13.7/11.1 months. Eighty percent of patients reported a subjective benefit. Accordingly, the R-value, ETS, and ETS-7 were significantly ( P < .05) improved. Tympanometry, CPT-AMA and ABG showed a positive trend, but the result was not significant. Tympanic retraction remained largely the same; a spontaneous closure of a chronic tympanic perforation was seen in 1 of 3 cases. Conclusions: The high subjective benefit and some significant objective improvement warrants further analysis of ETBD as part of the therapeutic management in pediatric CETD.
Estimation of Poverty Measures for Small Areas Under a Two-fold Nested Error Linear Regression Model: Comparison of Two Methods
Authors: Sohrabi Maryam , Rao J. N. K. 
Published: January 2024

Demand for reliable statistics at a local area (small area) level has greatly increased in recent years. Traditional area-specific estimators based on probability samples are not adequate because of small sample size or even zero sample size in a local area. As a result, methods based on models linking the areas are widely used. World Bank focused on estimating poverty measures, in particular poverty incidence and poverty gap called FGT measures, using a simulated census method, called ELL, based on a one-fold nested error model for a suitable transformation of the welfare variable. Modified ELL methods leading to significant gain in efficiency over ELL also have been proposed under the one-fold model. An advantage of ELL and modified ELL methods is that distributional assumptions on the random effects in the model are not needed. In this article, we extend ELL and modified ELL to two-fold nested error models to estimate poverty indicators for areas (say a state) and subareas (say counties within a state). Our simulation results indicate that the modified ELL estimators lead to large efficiency gains over ELL at the area level and subarea level. Further, modified ELL method retaining both area and subarea estimated effects in the model (called MELL2) performs significantly better in terms of mean squared error (MSE) for sampled subareas than the modified ELL retaining only estimated area effect in the model (called MELLI). AMS Subject Classification: 62D05, 62G09
Energy Consumption Displays in Electric Vehicles: Differential Effects on Estimating Consumption and Experienced Energy Dynamics Awareness
Authors: Gödker Markus , Moll Vivien E. , Franke Thomas 
Published: January 2024

Objective The effects of three prototypical designs of energy consumption displays on energy-specific situation awareness were examined. Background Energy efficiency is crucial for the sustainability of technical systems. However, without accurate situation awareness of energy dynamics (energy dynamics awareness, EDA) it can be challenging for humans to optimize the use of energy resources of electric vehicles (EVs) through their behavior. Method We examined three prototypical energy display designs that varied by their informational value to support EDA. Furthermore, we investigated the differential effects on EDA measured by (1) a newly constructed scale (experienced EDA), (2) estimating energy consumption, and (3) identifying efficient trips in an online experiment. Participants ( N = 82) watched standardized driving scenes (videos) of EV trips presenting the energy displays. Results We found a strong effect of display type on experienced EDA, with the trace display being the most supportive. The EDA scale showed excellent internal consistency. The consumption estimation and efficient trip identification indicators were not affected by the display type. Conclusion The study indicates that experienced EDA is immediately affected by displays with higher information value, but performance might need more time and training. More research is needed to investigate the cognitive processes related to EDA and to examine how distinct display elements enhance EDA. Application Results from this research can be used as guidance for the design of energy displays, especially in EVs. The EDA scale can be used as an evaluation measure in the human-centered design process of energy displays.
Manufacturing Productivity Measurement in India: An Evolutionary Trend
Authors: Joshi D. P. Priyadarshi , Gopinathan R. 
Published: January 2024

We undertake an extensive literature survey and segregate the methods mainly into two approaches: parametric and non-parametric. Further, we categorise the non-parametric techniques into the Growth Accounting Approach and the Deterministic Frontier Approach. The evaluation and development of methods used in the study of productivity growth measurement is a continuous process over the years, even if we found a longstanding critical debate from the classical school, popularly known as the Cambridge controversy. This article presents a holistic review of methods for documenting the growth debates in independent India, which helps understand the concepts for the new researcher in the field and the policymakers in the developing and less developed countries. JEL Codes: D24, E24, O47
Could breast multiparametric MRI discriminate between pure ductal carcinoma in situ and microinvasive carcinoma?
Authors: Rizzo Veronica , Cicciarelli Federica , Galati Francesca , Moffa Giuliana , Maroncelli Roberto , Pasculli Marcella , Pediconi Federica 
Published: January 2024

Background Ductal carcinoma in situ (DCIS) is often reclassified as invasive cancer in the final pathology report of the surgical specimen. It is of significant clinical relevance to acknowledge the possibility of underestimating invasive disease when utilizing preoperative biopsies for a DCIS diagnosis. In cases where such histologic upgrades occur, it is imperative to consider them in the preoperative planning process, including the potential inclusion of sentinel lymph node biopsy due to the risk of axillary lymph node metastasis. Purpose To assess the capability of breast multiparametric magnetic resonance imaging (MP-MRI) in differentiating between pure DCIS and microinvasive carcinoma (MIC). Material and Methods Between January 2018 and November 2022, this retrospective study enrolled patients with biopsy-proven DCIS who had undergone preoperative breast MP-MRI. We assessed various MP-MRI features, including size, morphology, margins, internal enhancement pattern, extent of disease, presence of peritumoral edema, time-intensity curve value, diffusion restriction, and ADC value. Subsequently, a logistic regression analysis was conducted to explore the association of these features with the pathological outcome. Results Of 129 patients with biopsy-proven DCIS, 36 had foci of micro-infiltration on surgical specimens and eight were diagnosed with invasive ductal carcinoma (IDC). The presence of micro-infiltration foci was significantly associated with several MP-MRI features, including tumor size ( P <0.001), clustered ring enhancement ( P <0.001), segmental distribution ( P <0.001), diffusion restriction ( P = 0.005), and ADC values <1.3 × 10−3 mm2/s ( P = 0.004). Conclusion Breast MP-MRI has the potential to predict the presence of micro-infiltration foci in biopsy-proven DCIS and may serve as a valuable tool for guiding therapeutic planning.
An exploration into the occupational identity of women following breast cancer and treatment: A qualitative study
Authors: Hopkins Courtney , Murphy Angela , Haythorne Rebecca , Cezar da Cruz Daniel 
Published: January 2024

Introduction: The number of women surviving after breast cancer is increasing, along with the length of time they are living with the after-effects of treatment. Although the treatment’s effects are known to impact occupational participation, little is known about how breast cancer could affect occupational identity. This study aims to illuminate the lived experience of women long-term after breast cancer treatment through an occupational perspective in order to explore how they perceive their occupational identity. Methods: A qualitative study with semi-structured interviews was conducted with six women, who had all received a diagnosis of breast cancer and treatment for longer than a year. Reflexive Thematic Analysis was used to analyse the data. Findings: Three intertwined themes describe the participants’ experience. (1) ‘Disruptions in daily life and Environmental support’, (2) ‘Be able to do’ and identity, and (3) ‘Doing what matters and is possible’. Findings revealed that the occupational identities of the participants were maintained. Cancer treatment effects appear to impact occupational competence that corresponded to participants’ occupational identities, suggesting difficulties in the order of occupational adaptation. Conclusion: Our findings contribute to understanding the challenges to occupational participation related to the occupational identity of women following breast cancer and treatment.
In Vivo Analysis of Acromioclavicular Kinematics and Distance During Multiplanar Humeral Elevation
Authors: Zhu Siyuan , Chen Yuzhou , Wang Peng , Shi Lewis L. , Li Guoan , Li Xinning , Koh Jason L. , Wang Shaobai , Yuan Tsai Tsung , Cui Guoqing , Wang Shaojie , Shao Xiexiang , Wang Jianhua 
Published: January 2024

Background: Knowledge of acromioclavicular (AC) joint kinematics and distance may provide insight into the biomechanical function and development of new treatment methods. However, accurate data on in vivo AC kinematics and distance between the clavicle and acromion remain unknown. Purpose/Hypothesis: The purpose of this study was to investigate 3-dimensional AC kinematics and distance during arm elevation in abduction, scaption, and forward flexion in a healthy population. It was hypothesized that AC kinematics and distance would vary with the elevation angle and plane of the arm. Study Design: Controlled laboratory study. Methods: A total of 19 shoulders of healthy participants were enrolled. AC kinematics and distance were investigated with a combined dual fluoroscopic imaging system and computed tomography. Rotation and translation of the AC joint were calculated. The AC distance was measured as the minimum distance between the medial border of the acromion and the articular surface of the distal clavicle (ASDC). The minimum distance point (MDP) ratio was defined as the length between the MDP and the posterior edge of the ASDC divided by the anterior-posterior length of the ASDC. AC kinematics and distance between different elevation planes and angles were compared. Results: Progressive internal rotation, upward rotation, and posterior tilt of the AC joint were observed in all elevation planes. The scapula rotated more upward relative to the clavicle in abduction than in scaption ( P = .002) and flexion ( P = .005). The arm elevation angle significantly affected translation of the AC joint. The acromion translated more laterally and more posteriorly in scaption than in abduction ( P < .001). The AC distance decreased from the initial position to 75° in all planes and was significantly greater in flexion ( P < .001). The MDP ratio significantly increased with the elevation angle ( P < .001). Conclusion: Progressive rotation and significant translation of the AC joint were observed in different elevation planes. The AC distance decreased with the elevation angle from the initial position to 75°. The minimum distance between the ASDC and the medial border of the acromion moved anteriorly as the shoulder elevation angle increased. Clinical Relevance: These results could serve as benchmark data for future studies aiming to improve the surgical treatment of AC joint abnormalities to restore optimal function.