bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding children’s behavioral health outcomes: A story of overcoming trauma and building strengths
Authors: McAllister Jordan H. , Vsevolozhskaya Olga A. , Tong Xiaoran , Lakin Daniel P. , Fairhurst Scott K. , Lyons John S. 
Published: January 2024

BackgroundAmong children enrolled in behavioral health treatment, those with multiple trauma experiences (known as Adverse Childhood Experiences, or ACEs) typically see worse outcomes. In this study, we examine whether having or building strengths can help such children become more resilient and experience better outcomes.ObjectiveWe examined the relationship between children’s traumatic experiences, strengths, and clinical improvement, testing whether building strengths can help reduce the negative impact of ACEs on children’s response to treatment.Participants and SettingWe used data from an evidence-based assessment to understand the clinical and functional needs and strengths of 5,423 children (ages 6-20) receiving treatment between 2019 and 2022 within a large community agency located in California.MethodsTo classify children by both level and rate of improvement, we relied on machine learning and principal components analysis. To determine the relationships between ACEs, strengths, and improvement, we used a variety of predictive models and descriptive analyses.ResultsAfter classifying children as being either “Faster”, “Slower”, or “Minimal” improvers, our analyses revealed that while higher total ACEs increases the likelihood of being a Slower improver, this effect can be mitigated by building strengths.ConclusionsThese results suggest that children with more ACEs are likely to require a longer duration of treatment before improvement is seen. They also suggest that promoting resilience— specifically focusing on building strengths—may lead to more efficient and effective care, particularly for children with significant trauma histories.
IntelliR: A comprehensive and standardized pipeline for automated profiling of higher cognition in mice
Authors: Gastaldi Vinicius Daguano , Hindermann Martin , Wilke Justus BH , Ronnenberg Anja , Arinrad Sahab , Kraus Sabine , Wildenburg Anne-Fleur , Ntolkeras Antonios , Provost Micah J , Ye Liu , Curto Yasmina , Cortés-Silva Jonathan-Alexis , Butt Umer Javed , Nave Klaus-Armin , Woznica Miskowiak Kamilla , Ehrenreich Hannelore 
Published: January 2024

ABSTRACTIn the rapidly evolving field of rodent behavior research, observer-independent methods facilitate data collection within a social, stress-reduced, and thus more natural environment. A prevalent system in this research area is the IntelliCage, which empowers experimenters to design individual tasks and higher cognitive challenges for mice, driven by their motivation to access reward. The extensive amount and diversity of data provided by the IntelliCage system explains the growing demand for automated analysis among users. Here, we introduce IntelliR, a standardized pipeline for analyzing raw data generated by the IntelliCage software, as well as novel parameters including the cognition index, which enables comparison of performance across various challenges. With IntelliR, we provide the tools to implement and automatically analyze 3 challenges that we designed, encompassing spatial, episodic-like, and working memory with their respective reversal tests. Using results from 3 independent control cohorts of adult female wildtype mice, we demonstrate their ability to comprehend and learn the tasks, thereby improving their proficiency over time. To validate the sensitivity of our approach for detecting cognitive impairment, we used adult female NexCreERT2xRosa26-eGFP-DTA mice after tamoxifen induced diphtheria toxin-mediated ablation of pyramidal neurons in cortex and hippocampus. We observed deterioration in learning capabilities and cognition index across several tests. IntelliR can be readily integrated into and adapted for individual research, thereby improving time management and reproducibility of data analysis.HIGHLIGHTSIntelliR is a standardized pipeline for analyzing raw data of IntelliCage software.Domains include spatial, episodic-like, and working memory with reversals.WT mice (3 cohorts) comprehend, learn and improve proficiency over time.Cognition index permits comparison of performance across cognitive domains.Mice with ablation of pyramidal neurons decline mainly in working memory.
High-parametric protein maps reveal the spatial organization in early-developing human lung
Authors: Sariyar Sanem , Sountoulidis Alexandros , Hansen Jan Niklas , Salas Sergio Marco , Mardamshina Mariya , Martinez Casals Anna , Navarro Frederic Ballllosera , Andrusivova Zaneta , Li Xiaofei , Czarnewski Paulo , Lundeberg Joakim , Linnarsson Sten , Nilsson Mats , Sundström Erik , Samakovlis Christos , Lundberg Emma , Ayoglu Burcu 
Published: January 2024

ABSTRACTThe respiratory system, including the lungs, is essential for terrestrial life. While recent research has advanced our understanding of lung development, much still relies on animal models and transcriptome analyses. In this study conducted within the Human Developmental Cell Atlas (HDCA) initiative, we describe the protein-level spatiotemporal organization of the lung during the first trimester of human gestation. Using high-parametric tissue imaging with a 30-plex antibody panel, we analyzed human lung samples from 6 to 13 post-conception weeks, generating data from over 2 million cells across five developmental timepoints. We present a resource detailing spatially resolved cell type composition of the developing human lung, including proliferative states, immune cell patterns, spatial arrangement traits, and their temporal evolution. This represents an extensive single-cell resolved protein-level examination of the developing human lungs and provides a valuable resource for further research into the developmental roots of human respiratory health and disease.
Self-adjusting Engineered Probiotic for Targeted Tumor Colonization and Local Therapeutics Delivery
Authors: Zou Zhen-Ping , Wang Xin-Ge , Sun Shu-Ting , Mi Jing , Zhang Xiao-Peng , Yin Bin-Chen , Zhou Ying , Ye Bang-Ce 
Published: January 2024

SUMMARYEngineered bacteria have demonstrated great potential for treating a broad array of tumors. However, the precision and safety of controlling the performance of engineered bacteria in vivo remains a central challenge. Here, we utilized genetic circuit programming strategy to construct an engineeredEscherichia coliNissle 1917 with accurate targeted colonizing and on-demand payloads releasing ability. The engineered probiotic survives only in the presence of more than 5 mM L-lactate by employing an improved lactate-sensing system, which leads to preventing the growth outside the permissive environments in mice. Meanwhile we introduce an expressing α-hemolysin (SAH) circuit based on quorum-sensing system to augment anti-tumor effect. Furthermore, coagulase induced by high-level lactate creates the closure to deprive tumor of nutrients and oxygen and prevents leakage of bacteria and SAH, which enhances the therapeutic effectiveness and biosafety. This self-adjusting living biotherapeutics significantly inhibits tumor proliferation and prolongs the survival time of colorectal tumor-bearing mice. Together, our work takes a step towards safer and more effective application of living bacteria for tumor treatment in practice.
Breaking free from the clock’s tyranny restores memory to brain damaged flies
Authors: Dissel Stephane , Morgan Ellen , Cao Lijuan , Wakefield Zachary Peters , Shetty Shohan , Chan Dorothy , Duong Vincent , Donlea Jeff , Farah Hamza , Loutrianakis Vasilios , Ford Melanie , Streett Lillith , Periandri Erica , Li Zhaoyi , Huang Irene , Abdala Dina , Kalra Arjan , Sousani Lea , Holder Brandon , McAdams Chloe , van Swinderen Bruno , Shaw Paul J. 
Published: January 2024

AbstractThe relationship between sleep and memory is an active topic of investigation. In this context, we demonstrate that enhancing sleep restores memory to flies with ablated Mushroom Bodies (MB), a key memory center; this is consistent across several memory assays. Mapping the underlying circuitry reveals circadian modulation of a subset of Dopaminergic neurons (DANs) that modulate aversive learning. Using imaging, we show that MB-ablation disrupts, and sleep restores the time of day these neurons are most responsive. Knocking down the receptor for the clock output signal,Pigment-dispersing factor(Pdfr), in this subset of DANs restores memory to MB-ablated flies. Crucially, MB-ablation does not result in memory impairments in the absence of a functioning clock. Our results reveal neuromodulation’s key role in cognitive restoration, where sleep aids memory in damaged brains, but a functioning clock unexpectedly hinders this process.
The translocation activity of Rad54 reduces crossover outcomes during homologous recombination
Authors: Sridalla Krishay , Woodhouse Mitchell V. , Hu Jingyi , Scheer Jessica , Ferlez Bryan , Crickard J. Brooks 
Published: January 2024

AbstractHomologous recombination (HR) is a template-based DNA double-strand break repair pathway that requires the selection of an appropriate DNA template for repair during the homology search stage of HR. Failure to execute the homology search quickly and efficiently can result in complex intermediates that generate genomic rearrangements, a hallmark of human cancers. Rad54 is an ATP dependent DNA motor protein that functions during the homology search by regulating the recombinase Rad51. How this regulation reduces genomic rearrangements is currently unknown. To better understand how Rad54 can prevent genomic rearrangements, we evaluated several amino acid mutations in Rad54 that were found in the COSMIC database. COSMIC is a collection of amino acid mutations identified in human cancers. These substitutions led to reduced Rad54 function and the discovery of a conserved motif in Rad54. Through genetic, biochemical, and single-molecule approaches, we show that disruption of this motif leads to failure in stabilizing early strand invasion intermediates, causing loss-of-heterozygosity rearrangements. Our study also suggests that the translocation rate of Rad54 is a determinant in balancing genetic exchange. This mechanism is likely fundamental to eukaryotic biology.
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development
Authors: Bulger Emily A. , McDevitt Todd C. , Bruneau Benoit G. 
Published: January 2024

ABSTRACTProper regulation of gene dosage is critical for the development of the early embryo and the extraembryonic tissues that support it. Specifically, loss ofCdx2 in vivoleads to stunted development of the allantois, an extraembryonic mesoderm-derived structure critical for nutrient delivery and waste removal in the early embryo. In this study, we investigate how CDX2 dose-dependently influences the gene regulatory network underlying extraembryonic mesoderm development. We generate an allelic series forCDX2in human induced pluripotent stem cells (hiPSCs) consisting of WT, heterozygous, and homozygous nullCDX2genotypes, differentiate these cells in a 2D gastruloid model, and subject these cells to multiomic single nucleus RNA and ATAC sequencing. We identify several genes that CDX2 dose-dependently regulate cytoskeletal integrity and adhesiveness in the extraembryonic mesoderm population, including regulators of the VEGF, canonical WNT, and non-canonical WNT signaling pathways. Despite these dose-dependent gene expression patterns, snATAC-seq reveals that heterozygous CDX2 expression is capable of inducing a WT-like chromatin accessibility profile, suggesting accessibility is not sufficient to drive gene expression when the CDX2 dosage is reduced. Finally, because the loss of CDX2 or TBXT phenocopy one anotherin vivo, we compare differentially expressed genes in our CDX2 knock-out model to those from TBXT knock-out hiPSCs differentiated in an analogous experiment. This comparison identifies several communally misregulated genes that are critical for cytoskeletal integrity and tissue permeability, includingANK3andANGPT1. Together, these results clarify how CDX2 dose-dependently regulates gene expression in the extraembryonic mesoderm and suggest these genes may underlie the defects in vascular development and allantoic elongation seen in the absence or reduction of CDX2in vivo.Summary StatementUsing 2D human gastruloids, CDX2 is shown to dose-dependently influence genes related to tissue permeability, cell-cell adhesions, and cytoskeletal architecture during extraembryonic mesoderm development.
Epitranscriptomic Reader YTHDF2 Regulates SEK1(MAP2K4)-JNK-cJUN Inflammatory Signaling in Astrocytes during Neurotoxic Stress
Authors: Malovic Emir , Ealy Alyssa , Hsu Phillip J. , Sarkar Souvarish , Miller Cameron , Rokad Dharmin , Goeser Cody , Hartman Aleah Kristen , Zhu Allen , Palanisamy Bharathi , Zenitsky Gary , Jin Huajun , Anantharam Vellareddy , Kanthasamy Arthi , He Chuan , Kanthasamy Anumantha G. 
Published: January 2024

AbstractAs the most abundant glial cells in the CNS, astrocytes dynamically respond to neurotoxic stress, however, the key molecular regulators controlling the inflammatory status of these sentinels during neurotoxic stress have remained elusive. Herein, we demonstrate that the m6A epitranscriptomic mRNA modification tightly regulates the pro-inflammatory functions of astrocytes. Specifically, the astrocytic neurotoxic stresser, manganese (Mn), downregulated the m6A reader YTHDF2 in human and mouse astrocyte cultures and in the mouse brain. Functionally, YTHDF2 knockdown augmented, while its overexpression dampened, neurotoxic stress induced proinflammatory response, suggesting YTHDF2 serves as a key upstream regulator of inflammatory responses in astrocytes. Mechnistically, YTHDF2 RIP-sequencing identifiedMAP2K4(MKK4;SEK1) mRNA as a YTHDF2 target influencing inflammatory signaling. Our target validation revealed Mn-exposed astrocytes mediates proinflammatory response by activating the phosphorylation of SEK1, JNK, and cJUN signaling. Collectively, YTHDF2 serves a key upstream ‘molecular switch’ controlling SEK1(MAP2K4)-JNK-cJUN proinflammatory signaling in astrocytes.
Bipolar thermoelectric superconducting single-electron transistor
Authors: Battisti Sebastiano , De Simoni Giorgio , Chirolli Luca , Braggio Alessandro , Giazotto Francesco 
Published: January 2024

Thermoelectric effects in normal metals and superconductors are usually very small due to the presence of electron-hole symmetry. Here, we show that superconducting junctions brought out of equilibrium manifest a sizable bipolar thermoelectric effect that stems from a violation of the detailed balance determined by the crucial role of the interactions at the mean-field level. To fully control the effect, we consider a thermally biased junction where the capacitance of the central S′ region is small enough to establish a Coulomb blockade regime. By exploiting charging effects we are able to tune the Seebeck voltage, the thermocurrent, and thereby the power output of this structure, via an external gate voltage. We then analyze the main figures of merit of bipolar thermoelectricity and we prospect for possible applications.

Published by the American Physical Society
2024
Assessing the need for a deregulation of the insurance sector of Mauritius to combat money laundering: a comparative study with Singapore and UK
Authors: Mahadew Bhavna 
Published: January 2024

Purpose
The purpose of this paper is to assess the current legal framework on money laundering control in the insurance sector. Essentially, this examination is premised on the interrogation of whether it is still appropriate for Mauritius to apply such stringent, opaque and unyielding Anti-Money Laundering/Combating Financing of Terrorism norms and rules on general insurance when developed nations such as the UK and Singapore have done away with them for a more effective combat against money laundering. It would also be assessed why the financial services commission (FSC) is not able to draw inspiration from its British and Singaporean counterparts in fighting money laundering more effectively.

Design/methodology/approach
This paper uses the doctrinal legal research methodology which is colloquially described as “black-letter law” approach. It is backed up by a contextual legal analysis that is based on an analysis of relevant legal provisions. It relies ground experience from the insurance industry through the experience of the authors. A comparative approach is used with Singapore and the UK as case studies given that there are significant commonalities to the Mauritian jurisdiction as well as useful differences.

Findings
It is observed that a move towards a de-regulation of the legal framework on money laundering in the insurance sector with a more relaxed approach is more effective for the Mauritian insurance sector. Evidence is drawn from the Singaporean and British models. A re-structuring of the FSC of Mauritius is also warranted for such an approach to be adopted.

Originality/value
This paper is among the first academic contribution that proposes a de-regulation and the adoption of a relaxed approach of and by the Mauritian Insurance Industry for a more effective combat against money laundering. It serves as a legal foundational basis for further research in this direction.
Disruptive innovation and e-micro-entrepreneurship in tourism: the IRMA model approach for Airbnb platform
Authors: Poulaki Ioulia , Chatzopoulou Evi , Constantoglou Mary , Konstantinidou Vaia 
Published: January 2024

PurposeThis paper aims to examine how Airbnb has been transformed from an informal form of tourism accommodation into an emerging form of tourism e-micro-entrepreneurship through an interesting triangle consisting of three distinct parts: hosts, platform and guests.Design/methodology/approachConsidering that the peer-to-peer response has sealed the sharing economy's success, research methodology involves primary research that focuses on the adeptness of Airbnb hosts as e-micro-entrepreneurs from the customers' perspective. A quantitative methodology was employed by applying a convenience sampling strategy through a structured questionnaire that was distributed online, resulting in a collection of 150 useable responses. A statistical analysis has been performed to test the research's objectives.FindingsDriven by Airbnb hosts' entrepreneurial behavior in managing their listings and guests' responses, research findings led to the development of a post-conceptual IRMA model, which describes this particular form of hosting as an e-micro-entrepreneurship opportunity, while guests' satisfaction confirms the platform's performance and hosts' efforts in service quality provision.Research limitations/implicationsThis study brings valuable insights to the tourism e-entrepreneurship literature through the assessment of the Airbnb platform and the hosts as e-micro-entrepreneurs, providing useful information to researchers and managers involved in the Sharing Economy's disruptive innovation and a more complete understanding of the drivers of Airbnb's consumer adoption.Originality/valueResearch on Airbnb mainly focuses on service quality from the customer perspective, while the existing literature does not highlight how a new type of e-micro-entrepreneurship has emerged by operating in the sharing economy's disruptive innovation ecosystem, which illustrates the factors that motivate hosts and guests to share accommodation services in an equilibrium bond.
Misery loves company: evaluation of negative e-WOM effects at the post-service recovery stage
Authors: Sengupta Aditi Sarkar , Stafford Marla Royne , Fox Alexa K. 
Published: January 2024

PurposeThe authors' research examines how negative electronic word-of-mouth (e-WOM) alters focal customers' post-recovery justice perceptions and attitudes to determine their future behavior with the service provider. Specifically, this paper develops and tests a conceptual model to investigate how negative e-WOM alters focal customers' perceptual and attitudinal outcomes after the service recovery experience. It also examines the post-recovery effect of negative e-WOM on focal customers’ willingness to patronize the service after their recovery experience.Design/methodology/approachTo test the hypotheses, two pretests and two experimental studies with created scenarios in the retail context were conducted.FindingsThe authors' findings reveal that services are judged during and well beyond failure and recovery occurrences. To maintain a loyal customer base, service managers should develop processes that address service complaints both within and beyond the service consumption stage. The authors also find that despite a favorable recovery, focal customers gravitate toward the failure experience and develop unfavorable attitudes toward the service provider, leading to likely defections.Originality/valueThe authors' research demonstrates the persuasive power of negative e-WOM at the post-service recovery stage, making a unique contribution to the service recovery literature. This research also contributes to the persuasive effect of negative e-WOM, demonstrating message context as a boundary condition of negative e-WOM effects. In general, the authors' work highlights the importance of understanding the psychological processes involved in eliciting the persuasive influence of negative e-WOM in the post-service recovery stage that may lead to the defection of “so-called” successfully recovered customers.
Using butterfly survey data to model habitat associations in urban developments
Authors: Cooper Joseph E. J. , Plummer Kate E. , Middlebrook Ian , Siriwardena Gavin M. 
Published: January 2024

AbstractOne approach for measuring the potential biodiversity in new urban construction projects is through ecological models that predict how wildlife will respond. For the United Kingdom, such models have only been developed for birds, but to maximise the extent to which models represent overall biodiversity, species from different indicator groups must be considered.Here, we assess this possibility for butterflies by combining citizen science survey data with high‐resolution digital maps. We derive detailed characteristics of urban landscapes around survey sites using previously established methods and quantify their relationship to counts of adults of 18 butterfly species in urban and peri‐urban settings.Higher butterfly counts were found when traversing urban sites with larger areas of semi‐natural grassland, other managed greenspaces and adjacent arable land. Most of the butterfly community were found to have negative relationships with highly built‐up or fragmented landscapes.We found high species‐specificity for different details of urban form, particularly in habitat elements such as gardens, vegetation around railways and grass verges.Policy implications. Improving biodiversity is now part of legislation governing new construction projects from England and Wales. However, predicting quantitative changes from hypothetical land‐use modifications remains challenging. Our models provide the foundation through which butterfly abundance could be integrated into an urban biodiversity assessment tool, providing species‐ and community‐level statistics to non‐specialists from the urban planning and design sector. This would allow them to hone configurations for built surfaces, private gardens, greenspaces and wildlife areas and assess their capacity to provide residents with the intended access to nature.