bluuebunny/crossref_metadata_2025_split huggingface dataset:
ApoNecV: A macro for cell death type differentiation
Authors: Kolarikova Marketa , Hosikova Barbora , Tesarik Jiri , Langova Katerina , Kolarova Hana 
Published: January 2025

AbstractThe evaluation of large experimental datasets is a fundamental aspect of research in every scientific field. Streamlining this process can improve the reliability of results while making data analysis more efficient and faster to execute. In biomedical research it is often very important to determine the type of cell death after various treatments. Thus, differentiating between viable, apoptotic, and necrotic cells provide critical insights into the treatment efficacy, a key aspect in the field of drug development. Fluorescent microscopy is perceived as a widely used technique for cell metabolism assessment and can therefore be used to investigate treatment outcomes after staining samples with cell death detection kit. However, accurate evaluation of therapeutic results requires quantitative analysis, often necessitating extensive postprocessing of imaging data. In this study, we introduce a complementary tool designed as a macro for the Fiji platform, enabling the automated postprocessing of fluorescent microscopy images to accurately distinguish and quantify viable, apoptotic, and necrotic cells.
Partial Sums of Almost Overdifferenced, Near‐Stationary Processes With Time‐Varying Properties
Authors: Demetrescu Matei , Hosseinkouchack Mehdi 
Published: January 2025

ABSTRACTThis note analyzes the limiting behavior of suitably normalized partial sums of processes with an ARMA(1,1) structure when the AR and MA roots are in various types of neighborhoods of 1 and , respectively. In doing so, we allow for time‐varying volatility and persistence. Our results show how the radii of neighborhoods in the MA and AR filters interact and affect the corresponding invariance principles.
Changes in marine iguana (Amblyrhynchus cristatus) heart rates suggest reduced metabolism during El Niño events
Authors: Dubiner S. , Pérez J. P. Muñoz , Alarcón‐Ruales D. , Cohen E. , Deresienski D. , Hirschfeld M. , Levin E. , Lohmann K. J. , Meiri S. , Lewbart G. 
Published: January 2025

AbstractMarine iguanas occasionally face severe food shortages because of algal dieback during El Niño events. Research on their adaptations to these periods has highlighted their unique ability to shrink in body length, which reduces their energetic needs. Additional mechanisms, like sustaining lower body temperatures and metabolic rates, could potentially also lower energy consumption, but have never been examined. We measured 665 iguanas over an 11‐year period including three El Niño events, and examined how heart rates (a proxy for metabolic rates) and body temperatures change with sea‐surface temperature oscillations (Oceanic Niño Index, ONI). Heart rate (adjusting for body size, temperature, season, and study site) was negatively correlated with ONI and lower during El Niño, whereas the adjusted body temperature did not correlate with ONI or differ between El Niño and other periods. We therefore hypothesize that marine iguanas can depress their metabolic rates in response to the harsh conditions, an adaptation that is complementary to shrinking and may further enhance their survival through periods of limited food. Direct metabolic measurements are needed to test this hypothesis.
Insights on the volcanic and impact histories of the lunar nearside from the petrology, geochemistry, and geochronology of the Calcalong Creek lunar regolith breccia meteorite
Authors: Oliveira B. H. , Snape J. F. , Tartèse R. , Pernet‐Fisher J. F. , van Acken D. , Whitehouse M. J. , Joy K. H. 
Published: January 2025

AbstractThe Calcalong Creek lunar meteorite is a regolith breccia with a lithologically diverse array of clasts set in a glassy, highly vesicular matrix. Here, we present a comprehensive new analysis of the meteorite. Comparisons to remote sensing data, lunar sample lithologies, and lunar sample ages indicate that it was likely sourced from regolith surrounding the Moon's nearside Procellarum KREEP Terrane, as opposed to the farside South Pole‐Aitken basin as has been previously suggested. Partial and complete reset dates of ~3.9 Ga suggest a disturbance at this time, which aligns with that of the Imbrium basin‐forming event, and to a lesser degree we see evidence of a ~4.2 Ga impact, which may be related to the formation of the Serenitatis basin. Analysis of Calcalong Creek clasts, thus, provide insights not only on the timing of major impact basin formation but also on the volcanic history of the Moon. The meteorite also samples some ancient ~4.3 Ga evolved magmatism, manifested in the presence of granophyre clasts, which may have originated from a high‐μ, KREEP‐like source, as well as younger, ~3.7 Ga low‐Ti basaltic magmatism.
VDAC1 Cleavage Promotes Autophagy in Renal Tubular Epithelial Cells With Hypoxia/Reoxygenation Injury
Authors: Chuan'ai Chen , Haolong Li , Pengpeng An , Yang Yu , Chunyan Liu , Yumiao Yang 
Published: January 2025

ABSTRACTAimTo study the effect and elucidate the underlying mechanisms of VDAC1‐ΔC on autophagy in renal tubular epithelial cells injured by hypoxia/reoxygenation.MethodsC57/BL6 mice were randomly divided into groups: sham operation group, IRI 1d group and IRI 2d group. The inner canthal blood of mice was collected to detect the levels of serum creatinine and urea nitrogen and kidney tissues were sampled, and sections were stained with Periodic acid‐Schiff for morphological evaluation. The expression of VDAC1 in kidney tissue was detected by Western blot. An immortalised human proximal tubular epithelial cell line, HK‐2 cells, were subjected to hypoxia/reoxygenation treatment. HK‐2 cells were incubated under hypoxia for 6 h, followed by 6 and 24 h of reoxygenation, cells were divided into four groups: H6/R0 group, H6/R6 group, H6/R24 group and control group. The release of LDH and cytosolic ROS were assessed, the expression of autophagy‐related proteins LC3 and p62 was detected by Western blot, autophagy flux was monitored by transfecting mRFP‐GFP‐LC3 lentivirus in HK2 cells, and cells were pretreated with bafilomycin A1 to further monitor the autophagy flux. VDAC1‐cleavage‐defective mutant in HK‐2 cells silencing VDAC1 was established to examine the effect of VDAC1 cleavage on autophagy and hypoxia/reoxygenation injury.ResultsIn vivo, IRI 1d/2d promoted the disorder of renal tubular structure and the cleavage of VDAC1 in kidney tissue; in vitro, hypoxia/reoxygenation promoted cytosolic ROS accumulation, LDH release, VDAC1 cleavage and induced autophagy and autophagic flux; reduced VDAC1 cleavage inhibited autophagy; and decreased cytosolic ROS accumulation and LDH release, thus alleviated cell injury.ConclusionIn renal tubular epithelial cells injured by H/R, VDAC1 cleavage was increased, triggering an autophagic response, and VDAC1 cleavage promoted autophagy to regulate cell injury.
Vegetation–climate feedbacks across scales
Authors: Miralles Diego G. , Vilà‐Guerau de Arellano Jordi , McVicar Tim R. , Mahecha Miguel D. 
Published: January 2025

AbstractVegetation is often viewed as a consequence of long‐term climate conditions. However, vegetation itself plays a fundamental role in shaping Earth's climate by regulating the energy, water, and biogeochemical cycles across terrestrial landscapes. It exerts influence by consuming water resources through transpiration and interception, lowering atmospheric CO2 concentration, altering surface roughness, and controlling net radiation and its partitioning into sensible and latent heat fluxes. This influence propagates through the atmosphere, from microclimate scales to the entire atmospheric boundary layer, subsequently impacting large‐scale circulation and the global transport of heat and moisture. Understanding the feedbacks between vegetation and atmosphere across multiple scales is crucial for predicting the influence of land use and land cover changes, and for accurately representing these processes in climate models. This review discusses the biophysical and biogeochemical mechanisms through which vegetation modulates climate across spatial and temporal scales. Particularly, we evaluate the influence of vegetation on circulation patterns, precipitation, and temperature, considering both long‐term trends and extreme events, such as droughts and heatwaves. Our goal is to highlight the state of science and review recent studies that may help advance our collective understanding of vegetation feedbacks and the role they play in climate.
Individualised Therapy for Obstructive Sleep Apnoea: Predictive Models and Anatomical Phenotyping of Mandibular Advancement Devices Responses
Authors: Camañes‐Gonzalvo Sara , Montiel‐Company José María , García‐Selva Marina , Plaza‐Espín Andrés , Pérez‐Carbonell Tomàs , Paredes‐Gallardo Vanessa , Bellot‐Arcís Carlos , Marco‐Pitarch Rocío 
Published: January 2025

ABSTRACTObjectivesThis non‐randomised clinical study aimed to identify the phenotypic characteristics that distinguish responders from non‐responders. Additionally, it sought to establish a predictive model for treatment response to obstructive sleep apnoea (OSA) using mandibular advancement devices (MAD), based on the analysed phenotypic characteristics.Material and MethodsThis study, registered under identifier NCT05596825, prospectively analysed MAD treatment over 6 years using two‐piece adjustable appliances according to a standardised protocol. Two response definitions aligned with the latest International Consensus Statement on OSA severity were established. Logistic regression and CHAID models integrated baseline clinical, anthropometric, cephalometric anatomical, soft tissue characteristics and physiological upper airway variables.ResultsA total of 112 patients completed the study: 64 responders and 48 non‐responders according to response definition 1, and 81 responders and 31 non‐responders according to response definition 2. Responders to MAD treatment had lower body mass index (BMI), neck and waist circumference, Epworth Sleepiness Scale scores, apnoea–hypopnea index (AHI), snoring intensity on the Visual Analog Scale, CPAP pressure, and higher T90% and minSaO2. Patients exhibiting greater anatomical imbalance, smaller airway volume, smaller minimum cross‐sectional area (CSAmin) and longer airway length demonstrated a poorer response to treatment.ConclusionsAirway length, initial T90% and anterior facial height collectively formed a highly predictive logistic regression model for response definition 1. Jarabak's ratio, gonial angle, CSAmin, airway length, initial BMI and baseline AHI constituted a highly predictive model for the second response definition. Furthermore, the CHAID regression tree established cutoff values for the variables that form the predictive models.
Cockroach pollination ensures sexual reproduction in the non‐photosynthetic plant Balanophora tobiracola
Authors: Suetsugu K. 
Published: January 2025

Abstract

While Coleoptera, Diptera, Hymenoptera, and Lepidoptera have traditionally been recognized as key pollinators, recent studies suggest that other insect groups, such as Blattodea (cockroaches), may also play a significant role. However, direct evidence of fruit set resulting from cockroach pollination remains limited, even in plants presumed to rely on this mode of pollination.

This study investigated the breeding system of the non‐photosynthetic plant Balanophora tobiracola on Yakushima Island, Japan, with a particular focus on the potential occurrence of agamospermy. Additionally, the research rigorously evaluates the pollination effectiveness of cockroaches and ants in B. tobiracola by assessing reproductive success exclusively through their visits.

Pollination experiments revealed high fruit set even under emasculation, indicating that agamospermy ensures seed production. However, B. tobiracola still relies heavily on pollinator activity for sexual reproduction. Ants and cockroaches were identified as effective pollinators, whereas contributions of pyralid moths, crane flies, and nitidulid beetles—whose larvae consume B. tobiracola infructescences—were negligible. Notably, a single visit from certain cockroach species significantly enhanced pollination.

The above findings demonstrate that B. tobiracola is effectively pollinated by cockroaches and ants. Combining unusual pollinators with agamospermy likely facilitates the reproductive success of B. tobiracola in dense forest understories where typical pollinators, like bees, are scarce. Our results underscore the need to reassess the role of agamospermy as reproductive assurance and the significance of cockroaches and ants as pollinators, particularly for plants in shaded understory habitats.
A Chromosome‐Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum
Authors: Toyokura Koichi , Naito Ken , Makabe Koyuki , Nampei Mami , Natsume Hiroki , Fukazawa Jutarou , Kusaba Makoto , Ueda Akihiro 
Published: January 2025

AbstractSalt stress disturbs plant growth and photosynthesis due to its toxicity. The ice plant Mesembryanthemum crystallinum is a highly salt‐tolerant facultative crassulacean acid metabolism (CAM) plant. However, the genetic basis of the salt tolerance mechanisms in ice plants remains unclear. In this study, we constructed a chromosome‐level whole‐genome sequence of the ice plant and performed transcriptome analysis of the effects of salt treatment on the leaves. After 24‐hour 500 mM NaCl treatment to the roots, 1100 and 1394 genes, including CAM pathway, glycolysis, and inositol metabolism, were up‐ and down‐regulated in the leaves, respectively. Salt treatment also influenced the abscisic acid (ABA) signaling components, including genes from the PYRABACTIN RESISTANCE1 (PYR1) family and the PROTEIN PHOSPHATASE 2CA (PP2CA) family. We detected the induction of the genes encoding various ion transporters after salt treatment. The expression of most v‐ATPase subunits is induced, leading to vacuolar acidification, which facilitates sodium ion sequestration in the vacuoles. Additionally, some genes encoding metal ion transporters, including the genes from the ZIP family and NRAMP family, were induced by salt treatment, accompanied by the accumulation of iron, zinc, and copper ions in the leaves. Cis‐motif enrichment analysis revealed that ABRE‐like motifs and MYB‐binding‐like motifs were enriched in the upstream sequences of genes that were either up‐regulated or down‐regulated by salt. In conclusion, this study highlights how salt treatment induces drastic and rapid transcriptomic changes and unveils the ice plant's genomic foundation. Our resources provide further insights into the regulation of salt tolerance in the ice plants.
Contrasts in hydraulics underlie the divergent performances of Populus and native tree species in water‐limited sandy land environments
Authors: Khan Attaullah , Gong Xue‐Wei , Zhang Chi , Liu Shen‐Si , Hao Guang‐You 
Published: January 2025

AbstractPopulus tree species are commonly used for creating shelter forests in vast areas of northern China, at least partially due to their fast growth. However, they are facing severe problems of decline and mortality caused by drought. In contrast, tree species native to water‐limited environments usually have slow growth and are currently not commonly used in afforestation, while these species are gaining more attention in forestry for their greater resilience to drought. In Horqin Sandy Land, we conducted a comparative analysis of xylem hydraulics and associated physiological traits between six Populus tree species and six tree species native to drought‐prone areas. Compared to the native species, the Populus species exhibited significantly higher stem hydraulic conductivity but lower resistance to drought‐induced xylem embolism than the native tree species. The observed interspecific variations and contrasts in xylem hydraulics between the two species groups were predominantly attributed to xylem anatomical characteristics at the pit level rather than at the tissue level. In line with the divergences in hydraulics, we found significantly lower intrinsic water use efficiency (WUEi) in Populus than in the native species, suggesting that the two groups adopted relatively acquisitive and conservative water use strategies, respectively. The trade‐off between hydraulic efficiency and safety, as well as that between hydraulic efficiency and WUEi, underlies the contrasts in performance between Populus species and the native tree species, that is, fast growth of Populus species but high risk of hydraulic dysfunction when facing drought, and vice versa.
Medium and Message by Mail: A Field Experiment to Promote Low‐Income Assistance Programs
Authors: Teodoro Manuel P. , Smith Jean 
Published: January 2025

ABSTRACTLimited awareness impedes take‐up in low‐income utility assistance programs, which often suffer from low uptake. This study uses a field experiment to evaluate direct mail as a means of reducing learning burdens and thereby increasing participation in financial assistance programs offered by a large American sewer utility. Employing a conjoint design, we sent customers mailings that varied by medium (postcard vs. letter), frequency, color, sender (government vs. nonprofit), message framing (percentage discount vs. dollar savings), and language (English vs. English‐Spanish). Compared to the control group receiving no mail, direct mail significantly increased program inquiries. Notably, postcards were as effective as letters, and sending even one mailing yielded a substantial response increase; additional mailings provided minimal benefit. Sender and message framing had no significant effect. Surprisingly, bilingual mailings resulted in slightly lower response rates. Our findings suggest that direct mailings, particularly postcards, can help improve take‐up of income‐qualified public assistance programs.
The new J‐curve testing methodology on the US–UK bilateral trade balance under the Brexit
Authors: Ongan Serdar , Karamelikli Huseyin , Gocer Ismet 
Published: January 2025

AbstractThis study reexamines the J‐curve hypothesis using a new methodology outside traditional practices. The increasing complexity of global bilateral trade and trade policy changes have made new methodologies inevitable. In response to this need, we create and use new forms of bilateral trade balances (BTB) rather than the traditional BTB ratio (exports/imports) constructed exclusively on total export data; this is because countries (e.g., the United States) also re‐export to other countries (e.g., the United Kingdom) some previously imported goods, besides their domestic goods. These new forms of BTBs are created based on this difference and named re‐export‐based BTB and domestic‐export‐based BTB. Hence, these newly created BTBs may also allow us to perform new J‐curve testing approaches, namely, domestic‐exports‐BTB‐based J‐curve hypothesis testing and re‐exports‐BTB‐based J‐curve hypothesis testing, separately. The main empirical finding suggests that J‐curve hypothesis testing for the USA should be performed with the methodology proposed in this study; this is because, while we supported the long‐run asymmetric J‐curve hypothesis by US domestic‐exports‐BTB‐based J‐curve hypothesis for 10 goods, the same was supported by US re‐exports‐BTB‐based J‐curve hypothesis for only 3. Additionally, the depreciated USD improves, and the Brexit process worsens the export of US domestically produced goods more than re‐exported goods.
Understanding Compliance Rates in Suicide Research During the Post‐Discharge Period
Authors: Schatten Heather T. , Wallace Gemma T. , Kimble Sara K. , Bozzay Melanie L. 
Published: January 2025

ABSTRACTIntroductionThe period following discharge from psychiatric hospitalization is one of particularly elevated suicide risk. It is essential to better understand risk factors for suicide during this period; however, retention and compliance in longitudinal research can be a challenge with high‐risk populations.MethodsWe examined compliance rates in the six‐month period following psychiatric hospital discharge among 174 adults (149 psychiatric patients and 25 healthy controls) across three data collection methods: ecological momentary assessment (EMA), weekly clinical assessment phone calls, and clinical follow‐up assessments at two‐ and six‐months post‐discharge. We examined whether clinical and demographic characteristics influenced compliance rates.ResultsResults suggested low rates of EMA compliance, but strong rates of completion of weekly phone calls and follow‐up assessments. Compared to psychiatric patients, healthy controls completed more EMA and weekly phone calls, but not follow‐up assessments. Participants who met current diagnostic criteria for a major depressive episode and who scored above the clinical threshold for borderline personality disorder symptoms had lower EMA compliance rates.ConclusionsThese findings have important implications for strategies to improve patient engagement in research during this high‐risk period.
Soil‐mediated effects of cover crops on weed‐crop competition
Authors: Rouge Alicia , Wallace John M. , Cordeau Stéphane , Moreau Delphine , Guillemin Jean‐Philippe , Lowry Carolyn J. 
Published: January 2025

AbstractAccording to the Resource Pool Diversity Hypothesis (RPDH), increasing cropping system diversity (e.g., crop rotation, fertility sources, and weed management) should increase soil resource pool diversity, and mitigate weed‐crop competition intensity. Despite the growing interest in cover crop mixtures to provide weed control, our study is the first to test the effect of cover crop species and functional diversity on weed‐crop competition intensity. We hypothesised that according to the RPDH, increasing cover crop species and functional diversity will mitigate weed‐crop competition intensity. Using soils collected from a long‐term field experiment comparing diverse cover crop mixtures to their mono‐species counterparts, we investigated in a greenhouse whether the legacy of cover crops in the soil would alter the intensity of competition that two competitive weeds can exert on maize. No relationship between the relative weed‐maize competition intensity and cover crop diversity was found, which failed to support our hypothesis. Nevertheless, the cover crop treatments varied in their effects on weed‐crop competition intensity, with the cover crop mixture consisting of triticale, Austrian winter pea and crimson clover resulting in the lowest weed‐crop competition intensity and highest maize biomass, regardless of the weed species competitor. Therefore, while cover crop legacies in the soil may affect weed‐crop competition, increasing diversity alone is not sufficient to reduce competition intensity. Future research should investigate potential mechanisms driving cover crop legacy effects on weed‐crop competition, especially in a field, where diversity in soil resources is likely to be greater compared to pots in a greenhouse.
In vitro
and
in vivo
activity of 1,2,3,4,6-O-pentagalloyl-glucose against
Candida albicans
Authors: Gao Lu , Wu Hao , Feng Jia , Liu Yu , Wang Ruina , Yan Lan , Lv Quanzhen , Jiang Yuanying 
Published: January 2025

ABSTRACT

Invasive fungal infections have become an increasingly serious threat to global human health, underscoring the urgent need for the development of new antifungal drugs. In this study, we found a natural polyphenolic compound 1,2,3,4,6-O-pentagalloyl-glucose (PGG), which is present in various plants and herbs. PGG showed broad-spectrum antifungal activities, enhancing the efficacy of fluconazole. Furthermore, PGG could protect mice against gastrointestinal and systemic infection with
Candida albicans
. Our mechanistic studies revealed that PGG exerts its antifungal effects partially by binding to the CaEno1 protein to inhibit its activity. As a crucial therapeutic target, Eno1 has been reported to be closely associated with cancer, hypertension, and infectious diseases. Our findings indicated that PGG, a new Eno1 inhibitor, is a potential candidate for further antifungal development.