bluuebunny/crossref_metadata_2025_split huggingface dataset:
The future of education in sleep science and medicine in Europe: Report of an ESRS workshop
Authors: McNicholas W. T. , Arnardottir E. S. , Vyazovskiy V. V. , Pevernagie D. , Penzel T. , Peigneux P. , Grote L. , Van Der Werf Y. , de Jongh F. , Schmidt M. , Adamantidis M. , Sekaran S. , Biller A. M. , Knobl B. , Luppi P.‐H. , Bassetti C. L. , Paunio T. 
Published: February 2025

SummaryThe European Sleep Research Society (ESRS) is the leading voice for sleep medicine and research in Europe and includes in its mission the objective to promote and develop sleep education. As part of this objective, the Society established a Multidisciplinary Task Force on sleep education, which proposed a workshop on this topic to define the role of ESRS in the future of sleep education in Europe. This includes defining the domains of operation by the ESRS, its most important links and networks, and to recognise areas for development. A concrete goal was to make an action plan with suggestions for eventual Task Forces. Recommendations from the workshop include active collaboration with other Societies and University degree courses in sleep with the objective to position the ESRS as the central focus for sleep education in Europe. Predefined learning objectives and Entrustable Professional Activities were identified as key aspects of future direction. The ESRS Textbook in Sleep Medicine provides a valuable educational resource, and the next edition should expand on the traditional format to include digital material and an integrative platform. The workshop also identified the need to develop a more compact publication to inform non‐sleep specialists, university students, and interested members of the public with basic information on sleep and sleep disorders.
The interaction between comorbidities and sleep stages influences oxygen re‐saturation characteristics
Authors: Howarth Timothy P. , Hietakoste Salla , Ebrahimian Serajeddin , Rissanen Marika , Kainulainen Samu , Karhu Tuomas 
Published: February 2025

SummarySleep stages exhibit differing patterns of cardiac, respiratory and nervous system activation. Rapid eye movement (REM) sleep is associated with deeper oxygen desaturation events in obstructive sleep apnea. However, no studies have looked to the effect of sleep staging on re‐saturation characteristics. Polysomnographic data from the Sleep Heart Health Study were utilised to derive oxygen saturation parameters from events having both desaturation and re‐saturation parts. Sleep stages were described as non‐REM (NREM) 1 or 2 (combined), 3 and REM. Sleep stage effects on desaturation and re‐saturation characteristics were investigated in a healthy subgroup (n = 759) and participants with hypertension (n = 2534), lung disease (n = 715), heart failure (n = 199) and myocardial infarction (n = 713). A total of 3793 participants (48.3% female) were included for analysis (171,976 saturation events; median 20 per participant). Events during REM had the longest duration, deepest nadirs and greatest area for desaturations and re‐saturations. Sleep stage effected re‐saturation parameters more than desaturation parameters, with a relative difference from NREM 1/2 to REM in duration of 16.7% and 29.8%, in rate of 0.2% and 4.5% and in area of 36.1% and 48.0% for desaturation and re‐saturation, respectively, among healthy participants. Similarly, the effect of comorbidities was greater upon re‐saturations than desaturations, as participants with heart failure recorded a relative difference to healthy participants of 10.3% and 24.4% for desaturation and re‐saturation duration, respectively, 12.8% and 15.0% for rate and 6.4% and 16.4% for area. Sleep stages and comorbidities have significant effects upon nocturnal oxygen re‐saturation parameters. Cardiorespiratory comorbidities elicit greater degradations in oxygen re‐saturations than they do desaturations.
Virulence of Candida spp. Isolates From Patients With Recurrent Vulvovaginal Candidosis Is Associated With the Number of Episodes
Authors: Consuegra‐Asprilla Jeiser Marcelo , Taborda Felipe , Pérez Verónica , Torres Brajhan , Rodríguez‐Echeverri Carolina , Muñoz Julián E. , González Ángel 
Published: February 2025

ABSTRACTBackgroundRecurrent vulvovaginal candidosis (RVVC) has been associated with increased antifungal resistance. Recently, we reported that Candida isolates from Colombian patients with RVVC did not show an increase in antifungal resistance.ObjectiveThe aim of this study was to evaluate the virulence of Candida isolates from patients with RVVC.MethodsA total of 40 Candida isolates were evaluated (37 C. albicans and 3 C. lusitaniae ). C. albicans isolates were divided into two groups based on the number of VVC episodes in patients per year: Group 1 (four to seven episodes; n = 26) and Group 2 (≥ eight episodes; n = 11). The XTT assay was used to assess biofilm formation. Galleria mellonella larvae were used for survival analysis and fungal load assessment, and the qPCR technique to determine the expression of the PRA1 gene.ResultsIt was observed that C. lusitaniae and C. albicans isolates from patients with ≥ eight VVC episodes per year exhibited a greater capacity to form biofilms compared to those from patients with four to seven VVC episodes. Moreover, in the G. mellonella model, larvae inoculated with isolates from RVVC patients exhibited approximately 80% mortality. Similarly, larvae infected with C. albicans from patients who experienced ≥ eight VVC episodes showed a significantly higher fungal load compared to the other evaluated groups; likewise, the expression of the PRA1 gene was significantly higher in isolates from patients with ≥ eight VVC episodes.ConclusionThese results indicate that Candida isolates from patients with RVVC exhibit a high degree of virulence and suggest that virulence may be one of the mechanisms explaining recurrence rather than antifungal resistance itself.
Manipulation of the brown glume and internode 1 gene leads to alterations in the colouration of lignified tissues, lignin content and pathogen resistance in wheat
Authors: Hua Lei , Song Rui , Hao Xiaohua , Zhang Jing , Liu Yanna , Luo Jing , Ren Xiaopeng , Li Hongna , Wang Guiping , Rehman Shams ur , Wu Jiajie , Fu Daolin , Dong Yuxiu , Wang Xiaodong , Zhang Chaozhong , Chen Shisheng 
Published: February 2025

SummaryLignin is a crucial component of the cell wall, providing mechanical support and protection against biotic and abiotic stresses. However, little is known about wheat lignin‐related mutants and their roles in pathogen defence. Here, we identified an ethyl methanesulfonate (EMS)‐derived Aegilops tauschii mutant named brown glume and internode 1 (bgi1), which exhibits reddish‐brown pigmentation in various tissues, including internodes, spikes and glumes. Using map‐based cloning and single nucleotide polymorphism (SNP) analysis, we identified AET6Gv20438400 (BGI1) as the leading candidate gene, encoding the TaCAD1 protein. The mutation occurred in the splice acceptor site of the first intron, resulting in a premature stop codon in BGI1. We validated the function of BGI1 using loss‐of‐function EMS and gene editing knockout mutants, both of which displayed reddish‐brown pigmentation in lignified tissues. BGI1 knockout mutants exhibited reduced lignin content and shearing force relative to wild type, while BGI1 overexpression transgenic plants showed increased lignin content and enhanced disease resistance against common root rot and Fusarium crown rot. We confirmed that BGI1 exhibits CAD activity both in vitro and in vivo, playing an important role in lignin biosynthesis. BGI1 was highly expressed in the stem and spike, with its localisation observed in the cytoplasm. Transcriptome analysis revealed the regulatory networks associated with BGI1. Finally, we demonstrated that BGI1 interacts with TaPYL‐1D, potentially involved in the abscisic acid signalling pathway. The identification and functional characterisation of BGI1 significantly advance our understanding of CAD proteins in lignin biosynthesis and plant defence against pathogen infection in wheat.
A novel microcapsule composite Spherulites Peony Superior Retinol mitigates UVB‐induced skin damage in vitro and in vivo
Authors: Han Jiejun , Gong Rongyue , Liu Yuankun , Gong Tiangui , Wang Bin , Zhang Laidi , Chen Jiayue 
Published: February 2025

AbstractSkin serves as our outermost barrier, protecting our bodies from various environmental damages. Increasing research has revealed that UVB is a primary factor for extrinsic aging. This study explored the role of a novel microcapsule composite Spherulites Peony Superior Retinol (SPSR) on skin damage induced by UVB. SPSR exhibited a capacity to eliminate UVB‐induced ROS. By measurement of cyclobutane pyrimidine dimers (CPD) and comet assay, the results implied that SPSR mitigates DNA damage from oxidative damage caused by UVB. In addition, UVB radiation typically leads to an increase in inflammatory factors within the skin. Decreased gene expressions of interleukin‐1α and TNF‐α have been observed in HaCaT cells. Moreover, a decreased gene expression of extracellular matrix (ECM)‐related protein, including fibronectin (FN1), Col1A1, and Col3A1 caused by UVB was mitigated by SPSR. Furthermore, the clinical trials with 30 volunteers confirmed the significant relief and antiwrinkle effects of the cosmetic formulation containing 0.1% SPSR. These findings implied the promising potential of SPSR as a comprehensive solution for combating the detrimental effects of UVB exposure and maintaining skin health.
How Meditation Promotes Well‐Being: Applying a Dual‐System Theory
Authors: Lehman Barbara J. , Fogel Camille A. , Wiederaenders Evan 
Published: February 2025

ABSTRACTMindfulness meditation and Buddhist‐informed contemplative approaches promote health and well‐being in part by helping meditators view present‐moment reality from a less immersed perspective. Contemplative practices build meta‐awareness and help practitioners psychologically distance from mental activity, thereby reducing negative emotions and craving while promoting mental flexibility. Metcalfe and Mischel's dual‐system model contrasts cool analytic processes with more emotional hot system processes. Contemplative practices facilitate adaptive coping in part by reducing hot and promoting cool system activation. Buddhist philosophies emphasizing the importance of present‐moment awareness, the futility of striving for self‐focused goals (worldly concerns), and the self‐perpetuating nature of negative emotions are used to explain how contemplative practices deter hot system activation and promote cool system activation over time.
Integrated STEM conceptualizations among teachers following a professional development program
Authors: Mesutoglu Canan , Akgündüz Devrim 
Published: February 2025

AbstractBecause teachers are now more often expected to develop integrated STEM lessons, it is essential to understand their conceptualizations to be able to support them effectively. This qualitative study explored teachers' integrated STEM conceptualizations upon participation in an engineering‐focused professional development program of 4 days. Data sources included written accounts to open‐ended questions collected from the 33 participating in‐service teachers and the STEM lesson plans the teacher teams developed during the program. The main emerging STEM characteristics were a focus on real‐world problems, engagement in engineering design, content integration, data practices, and STEM careers. Comparisons with the lesson plans and the further study of the conceptions through their distributions showed a limited attention for the broader local and social context, identifying and improving design solutions, engaging in reflection on STEM knowledge and skills, content integration with technology and mathematics knowledge and skills as opposed to assigning supportive roles, exposure to STEM careers and non‐STEM disciplines, and ways to foster a wide array of complex 21st century skills. Teachers can also be supported on the use of student‐centered pedagogies and problem solving.
Partial root‐zone drying irrigation enhances synthesis of glutathione in barley roots to improve low temperature tolerance
Authors: Mu Peng , Ye Fan , Liu Xintong , Zhang Peng , Liu Tianhao , Li Xiangnan 
Published: February 2025

SUMMARYPartial root‐zone drying irrigation (PRD) has been widely employed to regulate crop root development and responses to environmental fluctuations. However, its role in reprogramming rhizospheric microorganisms and inducing plant stress tolerance remains largely unexplored. This study aimed to investigate the effects of PRD on the response of barley (Hordeum vulgare) plants to low temperatures under various irrigation regimes. Under low temperature, barley plants subjected to PRD exhibited a significantly enhanced net photosynthetic rate, stomatal conductance, and maximum quantum efficiency of photosystem II compared to fully irrigated plants. Additionally, these plants showed a reduction in relative conductance. These results suggest that PRD could be a viable strategy for enhancing crop stress tolerance through irrigation management. Metabolomic analysis revealed that PRD influenced the accumulation of glutathione and 9‐octadecenamide in roots under low temperature, which was corroborated by transcriptome profiling data. Furthermore, the study highlighted the close association between this regulatory process and rhizosphere core microorganisms, such as Sphingobium and Mortierella, enriched in barley roots under PRD. This study revealed the mechanism underlying plant stress tolerance induction by PRD and the roles of rhizosphere microorganisms in this process. Also, the current study suggests that PRD is a promising strategy for enhancing crop stress tolerance through effective irrigation management.
Quality of whole blood stored in room temperature for up to 5 days
Authors: Susila Sanna , Helin Tuukka , Joutsi‐Korhonen Lotta , Lauronen Jouni , Ilmakunnas Minna 
Published: February 2025

AbstractBackgroundNon‐refrigerated whole blood can be used for bleeding emergencies when cold‐stored whole blood is unavailable. Storage time in room temperature is usually limited to 24 h although there is little evidence supporting this practice. We studied the quality of whole blood stored in room temperature for 5 days to investigate the effects of prolonged storage time.Study Design and MethodsNon‐leukoreduced whole blood in CPDA‐1 from 10 group O or A RhD positive male donors was stored in +22°C for 120 h. Samples were taken daily to assess blood cultures, blood count and metabolic parameters. Platelet function and blood coagulation were evaluated with multiple electrode aggregometry, viscoelastic tests (sonorheometry and rotational thromboelastometry), thrombin generation assay and measurements of individual clotting factors.ResultsBlood cell counts remained stable during storage. Metabolic changes were similar to those previously reported in cold‐stored blood products. Most coagulation factor levels, including FVIII, decreased during storage but remained within physiological range. Thrombin generation remained mostly intact during storage. In viscoelastic tests, clotting times prolonged, but clot strength remained stable. Platelet function in multiple electrode aggregometry impaired along with storage. No bacterial growth was detected in any sample.ConclusionWhole blood stored in room temperature for 5 days seems bacteriologically safe and retains most of its metabolic and hemostatic function. These results suggest that whole blood stored in room temperature may be usable for longer than the currently recommended 24 h.
An in silico simulation of the frequency of administering HLA‐incompatible low titer group O whole blood units when the donor pool includes unscreened female donors
Authors: Yazer Mark H. , Ngamsuntikul Samantha , Gandhi Manish , Apelseth Torunn , Taylor Audra , Seheult Jansen N. 
Published: February 2025

AbstractBackgroundAs low titer group O whole blood (LTOWB) increases in popularity, blood centers are finding innovative ways of maintaining the supply. One potential way is collecting LTOWB from parous female donors without testing for HLA antibodies. This in silico simulation predicted the risk of an LTOWB unit containing an HLA antibody and the subsequent risk for an HLA‐incompatible transfusion.MethodsAn LTOWB blood bank with 1 million units was simulated consisting of male, nulliparous, and parous female donors. The proportion of each donor type was modeled after the sex distribution at US blood centers. The parity of female donors was calculated based on the average number of live births per female depending on her age. HLA‐alloimmunization risk was determined by her parity status. The HLA haplotypes of the simulated recipients were derived from the 100 most common HLA haplotypes in the US National Marrow Registry Program database. The proportion of different race/ethnic groups in the US was used to simulate 100,000 LTOWB recipients to whom between 1 and 10 units were administered.ResultsOverall, the risk of an LTOWB unit containing at least one HLA antibody was 12.2% and the rate of receiving an HLA‐incompatible unit was 21.3%. The risk of receiving an HLA‐incompatible unit rose from 4.8% after receipt of one unit to 36.5% after 10 units.ConclusionBlood collectors and hospitals should evaluate the potential TRALI risk against the benefit of a potentially expanded inventory of LTOWB before collecting plasma‐containing products from non‐HLA‐tested parous female donors.
Textual evidence systematic reviews series paper 2: challenges and strategies in developing a search strategy for systematic reviews of textual evidence
Authors: Edwards Deborah , Cooper Adam , McArthur Alexa , Barber Brittany V. , Gregg Emily , Weeks Lori E. , Jordan Zoe 
Published: February 2025

Objective:
The objective of this paper is to highlight and address challenges as well as provide strategies for developing searches for systematic reviews of textual evidence.

Introduction:
When conducting a JBI review of textual evidence, it is important to consider different sources of published and unpublished material. While systematic search methodologies have been well-established for searching traditional peer-reviewed literature, applying those same rigorous methods to literature outside of academic journals can be more challenging. This paper highlights and addresses the challenges of developing searches for systematic reviews of textual evidence and provides strategies on how to conduct these. It takes into consideration the unique complexities of locating published material outside of academic journals and presents guidance for developing more robust searches incorporating textual evidence.

Discussion:
Researchers should acknowledge the value of textual evidence, including opinions, narratives, and policies, as crucial for informing health care practices. It is also essential to clearly define the types of textual evidence needed and establish comprehensive search parameters to ensure thorough coverage. To enhance the search process, researchers should follow a structured 3-phase approach: first, identify relevant keywords; second, conduct tailored searches in bibliographic databases; and third, perform supplementary searches. Furthermore, it is recommended they collaborate with information specialists and experts to refine and strengthen their search techniques. Researchers should also explore a variety of sources, including dedicated databases, conference proceedings, theses, dissertations, and media reports, to gather valuable textual evidence. Finally, it is important to systematically document all search processes to support transparency and reproducibility in the review.

Conclusion:
Searching broadly across bibliographic databases and including textual evidence from non-academic journals may provide the best available and most appropriate evidence to address specific questions.
Multi-Robot Scan-n-Print for Wire Arc Additive Manufacturing
Authors: Lu Chen-Lung , He Honglu , Ren Jinhan , Dhar Joni , Saunders Glenn , Julius Agung , Samuel Johnson , Wen John T. 
Published: February 2025

Abstract
Robotic Wire Arc Additive Manufacturing (WAAM) is a metal additive manufacturing technology offering flexible 3D printing while ensuring high-quality near-net-shape final parts. However, WAAM also suffers from geometric imprecision, especially for low-melting-point metals such as aluminum alloys. In this article, we present a multi-robot framework for WAAM process monitoring and control. We consider a three-robot setup: a 6-DoF welding robot, a 2-DoF trunnion platform, and a 6-DoF sensing robot with a wrist-mounted laser line scanner measuring the printed part height profile. The welding parameters, including the wire feed rate, are held constant based on the materials used, so the control input is the robot path speed. The measured output is the part height profile. The planning phase decomposes the target shape into slices of uniform height. During runtime, the sensing robot scans each printed layer, and the robot path speed for the next layer is adjusted based on the deviation from the desired profile. The adjustment is based on an identified model correlating the path speed to changes in height. The control architecture coordinates the synchronous motion and data acquisition between all robots and sensors. Using a three-robot WAAM testbed, we demonstrate significant improvements of the closed-loop scan-n-print approach over the current open loop result on both a flat wall and a more complex turbine blade shape.
Beneficiaries' Role in Averting Quality Challenges in Government Social Housing Construction
Authors: Amoah Christopher 
Published: February 2025

Abstract
Upon assuming office in 1994, the South African government began constructing social housing for the less privileged to improve their socioeconomic living standards. However, one of the primary factors behind the rising unhappiness with government-constructed social housing is the beneficiaries' lack of involvement. The study sought to identify beneficiaries' role in averting quality challenges in government social housing construction. A qualitative approach using random sampling was used to interview construction professionals with experience constructing government social housing. Online interviews were conducted with 29 participants, and the data were analyzed using a thematic content analysis method to generate themes and subthemes. The results indicate that to avert quality challenges in government-constructed social housing, the beneficiaries have a considerable role in the planning, construction, and postconstruction phases of the project implementation. Beneficiaries must be provided with the necessary documentation, prevent political interference, participate in the construction process, monitor progress, and train to maintain the houses after construction. The lack of participation of beneficiaries in government-constructed social housing leads to new housing construction with subpar quality standards, leading to the dissatisfaction of beneficiaries and the waste of public funds. There is a need to institute measures to enhance the beneficiaries' participation in the project implementation process to achieve the desired outcome. This study indicates how beneficiaries can partake in the development process of government-constructed social housing. The findings will enable beneficiaries to participate in ensuring satisfactory constructed social housing.