bluuebunny/crossref_metadata_2025_split huggingface dataset:
New interpretation technique for tracer well tests
Authors: Fedorov K. M. , Shevelev A. P. , Gilmanov A. Y. , Izotov A. A. , Kobyashev A. V. 
Published: January 2024

Background. Tracer well tests were proposed in the last century as an approach for elaboration of detailed reservoir models. Such tests implied injecting weakly adsorbable tracers into an injection well followed by collection and analysis of samples in nearby production wells. The results were assumed to clarify well connectivity and inter-well hydraulic conductivity. However, practical application of these tests showed that the tracer penetrates into the production well much faster than predicted from estimates of the reservoir properties.Aim. To develop a new technique for interpreting the results of tracer well tests.Materials and methods. The process of tracer movement along a self-induced hydraulic fracture was simulated taking into account the mass transfer parameter. The developed algorithm for interpreting the results of tracer studies was tested in a deposit located in Western Siberia. The productive interval is located in the Jurassic interval of the Vasyugan Formation. An aqueous solution of thiocarbamide with a concentration of 9% was used as a tracer. Injection was carried out through injection well XX74 for 3 h. The mass of the injected tracer was 1 t. Measurements in six reacting production wells XX72, XX73, XX75, XX76, XX77, and XX78 were conducted for 75 days. During this time period, 516 samples were collected. In the first three days, 36 samples were extracted at equal time intervals; in the following two weeks, 90 samples were extracted also at equal time intervals; during the remaining days, measurements were conducted 2—3 times per week for each well. The results of the conducted studies were interpreted based on the developed methodology for all the wells.Results. A numerical and analytical solution of the problem of tracer slug movement along the fracture was obtained. Account was taken of the mass transfer between the fluids and the layer, as well as the dissipation of the tracer slug. This solution was used as the basis for a new technique for interpreting the results of tracer well tests, which allows a greater number of fracture parameters to be determined.Conclusion. The developed technique for determining the parameters of technogenic fractures was applied in practice. The presence of several fractures connecting injection and production wells is shown. It is established that the process of formation of such fractures in the reservoir is still ongoing.
Analisis Perhitungan PPh final penjualan online sebelum dan sesudah mendapat insentif pajak penghasilan berdasarkan PMK No.9/PMK.03/2021 (Studi Kasus Pada I-support Kediri)
Authors: Melyana Jesi , Fauziyah Fauziyah , Isnaniati Siti 
Published: January 2024

Estimated tax revenue will increase from year to year. In addition to the number that continues to increase every year, taxes are also the largest source of state revenue. Therefore, it is important for taxpayers to deposit taxes into the state treasury in accordance with applicable regulations. This study aims to find out how to apply the final tax incentives in accordance with applicable regulations given by the government in response to Covid-19. In this study the data used are primary data and secondary data. The analytical method used is descriptive quantitative with data collection techniques in the form of interviews and documentation.
The results obtained that the application of tax incentives decreased the comparison of income tax in 2020 before receiving tax incentives was Rp. 19,724,164 and after receiving tax incentives was Rp. 9,859,082. Meanwhile, in 2021, it can be seen from the comparison that before receiving tax incentives it was Rp. 19,840,739 and after receiving it was 10,017,530. the difference before and after incentives in year to year is quite large, namely in 2020 it is 9,865,082 and in 2021 it is 9,823,208.
It is hoped that I-Support will be more thorough and updated with changes to existing tax regulations. I-Support is expected to be able to calculate its tax in accordance with the updated regulations. This research is expected to assist researchers in increasing academic knowledge and can provide benefits for other students in conducting research related to online sales income tax incentives.
Keywords: tax incentives, final tax, online sales tax
Analysis of Development of Shrimp Aquaculture In Secanggang District, Langkat Regency, Sumatera Utara Province
Authors: Rahmadhayani Anisa 
Published: January 2024

The marine and fisheries sector's development is a part of the national development of Indonesia. Therefore, it is essential to ensure the industries' sustainability. Aquaculture is an industry that has a tremendous opportunity. Currently, Indonesia's aquaculture prospects are rising. Langkat Regency is a region in Sumatera Utara Province located in the East Coastal Region with good prospects for brackish water aquaculture. There are four goals in this study. This study aimed to analyze the distribution of shrimp aqua farm locations in the Secanggang District, investigate the type of shrimp aquaculture in the district, analyze the economic aspects of shrimp aquaculture in Secanggang District and study the optimal aquaculture strategy as a priority for developing coastal areas in Secanggang District. This study uses a quantitative approach to conduct a descriptive study. The study shows that the aqua farms in Secanggang District are located in the production area, converted forest area, wildlife nature reserve, and production forest. There are four types of Shrimp aquaculture in Secanggang District: intensive, semi-intensive, traditional, and natural/organic. The study also shows the economic analysis on costs, income, and the efficiency of R/C ratios per cycle on four types of aquaculture. This study also shows that shrimp aquaculture using the intensive system technique is the most appropriate and priority for shrimp aqua farming development in Secanggang District
Spice production and marketing in Kaffa Biosphere Reserve, Ethiopia
Authors: Afeto Amanuel , Kassa Kefyalew , Belay Tesfaye , Gebremchael Abiy 
Published: January 2024

Ethiopia has favorable climate conditions for the production of spices, herbs, aromatic and medicinal plants useful for flavoring food, aroma, therapeutic and marketing for cash income. Ethiopian is the largest consumer of spices in Africa, but the contribution of spice to the national economy is meager. The study area is endowed with diversified spice types with different purposes. However, it is not well announced, attention is not given and still many spices are growing in the wild. However, it is not well announced, attention is not given, and many spices are still collected from the wild. This study aimed to assess spice production and marketing in Kaffa biosphere reserve. This study was undertaken in four districts of Kaffa zone including Tello, Gimbo, Shishoende and Decha districts by selecting one representative kebele from each district. The data collection method used for this study is a household survey, key informant interviews and focus group discussion. Out of the total sampled 89% of sampled households participate and produce diversified types of spice. Korarima, red pepper, rue, ginger, turmeric, bush tea, holey basil, machewolago, aemacho nechamacho, coriander, rosemary, onion, long pepper and lemon grass were identified at the household survey. About 60.9% of the sample households provide spice products to market and the sample households generate a mean income of 1,611.14 ETB from spice product marketing. Most sampled households ranked themselves at low and very low knowledge levels on spice production and marketing. Spices are mainly produced in the home garden area for consumption. The main challenges for spice production in the study area are the unavailability of improved variety, low attention given by government and stakeholders, unavailability of the market, lack of knowledge and skill on production and post-harvest handling, low-value addition practice and unavailability of recommended practice. Creating a market for spice products and promotion is needed its importance and give attention to spice production. Training is needed for the farming community on spice cultivation, management, post-harvest handling and processing.
Int. J. Agril. Res. Innov. Tech. 13(2): 22-30, Dec 2023
Green biosynthesis of bimetallic selenium–gold nanoparticles using Pluchea indica leaves and their biological applications
Authors: Aly Khalil Ahmed Mohamed , Saied Ebrahim , Mekky Alsayed E. , Saleh Ahmed M. , Al Zoubi Omar Mahmoud , Hashem Amr H. 
Published: January 2024

Increasing bacterial resistance and the negative impact of currently used antibacterial agents have produced the need for novel antibacterial agents and anticancer drugs. In this regard, nanotechnology could provide safer and more efficient therapeutic agents. The main methods for nanoparticle production are chemical and physical approaches that are often costly and environmentally unsafe. In the current study, Pluchea indica leaf extract was used for the biosynthesis of bimetallic selenium–gold nanoparticles (Se-Au BNPs) for the first time. Phytochemical examinations revealed that P. indica leaf extract includes 90.25 mg/g dry weight (DW) phenolics, 275.53 mg/g DW flavonoids, and 26.45 mg/g DW tannins. X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques were employed to characterize Se-Au BNPs. Based on UV-vis spectra, the absorbance of Se-Au BNPs peaked at 238 and 374 nm. In SEM imaging, Se-Au BNPs emerged as bright particles, and both Au and Se were uniformly distributed throughout the P. indica leaf extract. XRD analysis revealed that the average size of Se-Au BNPs was 45.97 nm. The Se-Au BNPs showed antibacterial properties against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, with minimum inhibitory concentrations (MICs) of 31.25, 15.62, 31.25, and 3.9 μg/mL, respectively. Surprisingly, a cytotoxicity assay revealed that the IC50 value toward the Wi 38 normal cell line was 116.8 μg/mL, implying that all of the MICs described above could be used safely. More importantly, Se-Au BNPs have shown higher anticancer efficacy against human breast cancer cells (MCF7), with an IC50 value of 13.77 μg/mL. In conclusion, this paper is the first to provide data on the effective utilization of P. indica leaf extract in the biosynthesis of biologically active Se-Au BNPs.
YAP mechanotransduction under cyclic mechanical stretch loading for mesenchymal stem cell osteogenesis is regulated by ROCK
Authors: Kim Eunju , Riehl Brandon D. , Bouzid Tasneem , Yang Ruiguo , Duan Bin , Donahue Henry J. , Lim Jung Yul 
Published: January 2024

While yes-associated protein (YAP) is now recognized as a potent mechanosensitive transcriptional regulator to affect cell growth and differentiation including the osteogenic transcription of mesenchymal stem cells (MSCs), most studies have reported the YAP mechanosensing of static mechanophysical cues such as substrate stiffness. We tested MSC response to dynamic loading, i.e., cyclic mechanical stretching, and assessed YAP mechanosensing and resultant MSC osteogenesis. We showed that cyclic stretching at 10% strain and 1 Hz frequency triggered YAP nuclear import in MSCs. YAP phosphorylation at S127 and S397, which is required for YAP cytoplasmic retention, was suppressed by cyclic stretch. We also observed that anti-YAP-regulatory Hippo pathway, LATS phosphorylation, was significantly decreased by stretch. We confirmed the stretch induction of MSC osteogenic transcription and differentiation, and this was impaired under YAP siRNA suggesting a key role of YAP dynamic mechanosensing in MSC osteogenesis. As an underlying mechanism, we showed that the YAP nuclear transport by cyclic stretch was abrogated by ROCK inhibitor, Y27632. ROCK inhibitor also impaired the stretch induction of F-actin formation and MSC osteogenesis, thus implicating the role of the ROCK-F-actin cascade in stretch-YAP dynamic mechanosensing-MSC osteogenesis. Our results provide insight into bone tissue engineering and skeletal regenerative capacity of MSCs especially as regards the role of dynamic mechanical loading control of YAP-mediated MSC osteogenic transcription.
Will previous antimicrobial therapy reduce the positivity rate of metagenomic next-generation sequencing in periprosthetic joint infections? A clinical study
Authors: Hao Linjie , Bian Weiguo , Qing Zhong , Ma Tao , Li Hui , Xu Peng , Wen Pengfei 
Published: January 2024

BackgroundMetagenomic next-generation sequencing (mNGS) is a culture-independent massively parallel DNA sequencing technology and it has been widely used for rapid etiological diagnosis with significantly high positivity rate. Currently, clinical studies on evaluating the influence of previous antimicrobial therapy on positivity rate of mNGS in PJIs are rarely reported. The present study aimed to investigate whether the positivity rate of mNGS is susceptible to previous antimicrobial therapy.MethodsWe performed a prospective trial among patients who undergone hip or knee surgery due to periprosthetic joint infection (PJI) to compare the positivity rate of culture and mNGS between cases with and without previous antimicrobial therapy, and the positivity rates between cases with different antimicrobial-free intervals were also analysed.ResultsAmong 131 included PJIs, 91 (69.5%) had positive cultures and 115 (87.8%) had positive mNGS results. There was no significant difference in the positivity rate of deep-tissue culture and synovial fluid mNGS between cases with and without previous antimicrobial therapy. The positivity rate of synovial fluid culture was higher in cases with previous antimicrobial therapy. The positivity rates of mNGS in synovial fluid decreased as the antimicrobial-free interval ranged from 4 to 14 days to 0 to 3 days.ConclusionmNGS is more advantageous than culture with a higher pathogen detection rate. However, our data suggested that antimicrobial agents may need to be discontinued more than 3 days before sampling to further increase the positivity rate of mNGS for PJIs.
Development of embodied listening studies with multimodal and wearable haptic interfaces for hearing accessibility in music
Authors: Cavdir Doga 
Published: January 2024

IntroductionThe intersection of hearing accessibility and music research offers limited representations of the Deaf and Hard of Hearing (DHH) individuals, specifically as artists. This article presents inclusive design practices for hearing accessibility through wearable and multimodal haptic interfaces with participants with diverse hearing backgrounds.MethodsWe develop a movement-based sound design practice and audio-tactile compositional vocabulary, co-created with a Deaf co-designer, to offer a more inclusive and embodied listening experience. This listening experience is evaluated with a focus group whose participants have background in music, dance, design, or accessibility in arts. By involving multiple stakeholders, we survey the participants' qualitative experiences in relation to Deaf co-designer's experience.ResultsResults show that multimodal haptic feedback enhanced the participants' listening experience while on-skin vibrations provided more nuanced understanding of the music for Deaf participants. Hearing participants reported interest in understanding the Deaf individuals' musical experience, preferences, and compositions.DiscussionWe conclude by presenting design practices when working with movement-based musical interaction and multimodal haptics. We lastly discuss the challenges and limitations of access barrier in hearing accessibility and music.
A multiscale geographically weighted regression kriging method for spatial downscaling of satellite-based ozone datasets
Authors: Cheng Shuang , Zhang Guoqiao , Yang Xuexi , Lei Bingfeng 
Published: January 2024

Accurate monitoring of ozone (O3) concentrations by remote sensing is essential for achieving pollution control and ecological protection. However, the existing O3 remote sensing data with a low spatial resolution do not facilitate fine-grained studies of small-scale urban clusters. In this study, the multiscale geographically weighted regression kriging (MGWRK) method was used to spatially downscale O3 remote sensing products (10 km × 10 km). Downscaling factors were selected from meteorological factors and vegetation, aerosol optical thickness (AOD), and air pollutant emission inventory data. Spatial heterogeneity and scale differences among the factors were considered and compared via multiple regression kriging (MLRK) and geographically weighted regression kriging (GWRK) to generate 1-km annual and seasonal O3 remote sensing products. The results showed that I) the downscaling accuracy of each model can be expressed as MGWRK &gt; GWRK &gt; MLRK; the local downscaling model yields data that are more consistent with the actual spatial distribution of O3 after considering the spatial heterogeneity of the influencing factors; and the downscaled annual and seasonal data exhibit satisfactory spatial texture characteristics and consistency with the original spatial distribution of O3, while the distribution boundary problem of image elements is eliminated. II) Nitrogen oxide (NOx) and volatile organic compound emissions and temperature exhibit strong positive correlations with O3, while wind speed, humidity, the normalized difference vegetation index, and AOD indicate weak positive correlations with O3. Moreover, precipitation exhibits a weak negative correlation with O3. III) The coefficient of determination (R2) of the 1-km resolution annual O3 concentration data after downscaling based on the MGWRK model reaches 0.93, while the RRMSE and MAE values are only 3% and 1.86, respectively, with a coefficient of variation of 9.55%; the downscaling accuracy of the seasonal O3 concentration data is higher in summer and winter than during the other seasons, with R2 greater than 0.85, further confirming the spatial and temporal downscaling advantages of the MGWRK model for O3 in the Chang-Zhu-Tan city cluster. This further corroborates the feasibility of the MGWRK model for spatial and temporal O3 downscaling in the Chang-Zhu-Tan urban area.
Development and deployment of an improved Anopheles gambiae s.l. field surveillance by adaptive spatial sampling design
Authors: Monteiro Gabriel M. , Djogbénou Luc S. , Donnelly Martin J. , Sedda Luigi 
Published: January 2024

IntroductionAccurate assessments of vector occurrence and abundance, particularly in widespread vector-borne diseases such as malaria, are crucial for the efficient deployment of disease surveillance and control interventions. Although previous studies have explored the benefits of adaptive sampling for identifying disease hotspots (mostly through simulations), limited research has been conducted on field surveillance of malaria vectors.MethodsWe developed and implemented an adaptive spatial sampling design in southwestern Benin, specifically targeting potential and uncertain Anopheles gambiae hotspots, a major malaria vector in sub-Saharan Africa. The first phase of our proposed design involved delineating ecological zones and employing a proportional lattice with close pairs sampling design to maximize spatial coverage, representativeness of ecological zones, and account for spatial dependence in mosquito counts. In the second phase, we employed a spatial adaptive sampling design focusing on high-risk areas with the greatest uncertainty.ResultsThe adaptive spatial sampling design resulted in a reduced sample size from the first phase, leading to improved predictions for both out-of-sample and training data. Collections of Anopheles gambiae in high-risk and low-uncertainty areas were nearly tripled compared to those in high-risk and high-uncertainty areas. However, the overall model uncertainty increased.DiscussionWhile the adaptive sampling design allowed for increased collections of Anopheles gambiae mosquitoes with a reduced sample size, it also led to a general increase in uncertainty, highlighting the potential trade-offs in multi-criteria adaptive sampling designs. It is imperative that future research focuses on understanding these trade-offs to expedite effective malaria control and elimination efforts.
Genotoxin-producing Salmonella enterica induces tissue-specific types of DNA damage and DNA damage response outcomes
Authors: Lopez Chiloeches Maria , Bergonzini Anna , Martin Océane C. B. , Bergstein Nicole , Erttmann Saskia F. , Aung Kyaw Min , Gekara Nelson O. , Avila Cariño Javier F. , Pateras Ioannis S. , Frisan Teresa 
Published: January 2024

IntroductionTyphoid toxin-expressing Salmonella enterica causes DNA damage in the intestinal mucosa in vivo, activating the DNA damage response (DDR) in the absence of inflammation. To understand whether the tissue microenvironment constrains the infection outcome, we compared the immune response and DDR patterns in the colon and liver of mice infected with a genotoxigenic strain or its isogenic control strain.MethodsIn situ spatial transcriptomic and immunofluorescence have been used to assess DNA damage makers, activation of the DDR, innate immunity markers in a multiparametric analysis.ResultThe presence of the typhoid toxin protected from colonic bacteria-induced inflammation, despite nuclear localization of p53, enhanced co-expression of type-I interferons (IfnbI) and the inflammasome sensor Aim2, both classic features of DNA-break-induced DDR activation. These effects were not observed in the livers of either infected group. Instead, in this tissue, the inflammatory response and DDR were associated with high oxidative stress-induced DNA damage.ConclusionsOur work highlights the relevance of the tissue microenvironment in enabling the typhoid toxin to suppress the host inflammatory response in vivo.