bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis reveals the contribution of amino acid transporters to acid tolerance in Lactococcus lactis
Authors: Zhu Zhengming , Zhang Tongrong , Yang Zhihan , Zhang Juan 
Published: January 2024

AbstractAmino acid transporters are promising targets for engineering acid‐tolerant strains of Lactococcus lactis. However, the simple overexpression of transporters alone is insufficient to achieve a highly acid‐resistant phenotype. This study investigated the effects of amino acid transporters on the acid‐stress tolerance of L. lactis. Here, we first verified the contribution of amino acid transporters to acid tolerance by overexpressing the ctrA, glnP, and glnQ genes in L. lactis. Transcriptome analysis revealed that most genes associated with specific amino acid transport, pyrimidine metabolism, and functional proteins, were upregulated in the overexpression strains. Among them, arginine biosynthesis (argG and argH), amino acid transport (yjeM and azlC), and pyrimidine metabolism were considered to be the most important regulatory mechanisms. Importantly, metabolite profiling revealed that the overexpression strains had higher intracellular levels of amino acids, particularly aspartate, glutamate, and arginine at low pH, as well as higher intracellular ATP levels, which was consistent with the corresponding gene‐expression levels. Finally, the simultaneous overexpression of glnP and glnQ led to a further improvement of acid tolerance in L. lactis. This study reveals the regulatory mechanisms of amino acid transporters, and provides a novel strategy for achieving higher acid tolerance via positive tandem expression approaches.
Sea bass (Lateolabrax maculatus) exerts anti‐ulcerative colitis effects through gut microbiota modulation
Authors: Chen Jiali , Xiao Yuhang , Wang Kejian , Fu Xiuqiong , Fan Yueyao , Hossen Muhammad Jahangir , Chen Yingjie , Bai Weibin , Yu Zhiling , Xu Baojun 
Published: January 2024

AbstractTraditional Chinese medicinal books have noted that sea bass (Lateolabrax maculatus) may effectively manage inflammation‐linked conditions. The folk use of sea bass during pregnancy as nutritional care is also popular in China. However, scientific verification of health benefits of sea bass remains a lack of report. Our previous studies have proven that sea bass has therapeutic benefits for ulcerative colitis (UC). However, a further investigation on the interacted mechanism among sea bass, UC, and gut microbiota has still not been fully explored and clarified. Hence, gut microbiota as the crucial mechanism of UC was evaluated. Gut ecosystem was analyzed by 16sRNA sequencing. The potential microbial biomarker was found through LEfSe analysis. Flow cytometry was then further applied to verify the identified biomarker. Results revealed that sea bass could effectively recover the abundance of UC‐mediated microbiota. LEfSe analysis found that Christensenellaceae could be identified as the potential biomarker for sea bass‐mediated gut microbiota in UC. Further in vitro study conjointly confirmed that the identified bioactive constituent of sea bass could significantly promote the growth of Christensenella minuta. Sufficient results derived that sea bass could ameliorate UC through gut microbiota modulation. C. minuta was identified as the promising microbial biomarker for the mechanism of sea bass in treating intestinal inflammation. It provided further elucidation on the pharmacological mechanism of sea bass in alleviating UC through gut microbiota modulation. It is a crucial driving force for the progress of scientific research on the dietary therapy of sea bass.
Research on optimization of spray drying conditions, characteristics of anthocyanins extracted from Hibiscus sabdariffa L. flower, and application to marshmallows
Authors: Hoang Nhon Thi Ngoc , Nguyen Nga Ngoc Kieu , Nguyen Ly Thi Kim , Le Anh Thi Hong , Dong Dao Thi Anh 
Published: January 2024

AbstractAnthocyanin, a main‐colored bioactive compound found in Hibiscus sabdariffa L., is well‐known for a varied range of applications as food additives in foodstuff, and natural colorants in food, pharmaceutical, and printing industries. The study aimed to find out the suitable conditions for the spray‐drying process to obtain anthocyanin powder from the extract as well as characterized the powder. In addition, the obtained powder was applied to marshmallows and determined the acceptability of appearance, quality, and scavenging capacity of the candy. The carrier of maltodextrin and gum arabic was selected for spray‐drying, which had optimal conditions at 144°C and 7 mL/min, resulting in 100.22 mg/g anthocyanin content with an encapsulation efficiency of 93.87%. The obtained anthocyanin has appropriate moisture of 5.14%, quite appropriate bulk density, and tapped density, it also was high solubility, and poor flowability but easy compression. The shape of the particle by SEM analysis was low particle size (2–10 μm), wrinkled, unequal spherical size, rough surfaces with indentations, and slight cracks. The X‐ray diffraction (XRD) spectrum of the sample had very low crystallinity and diffuse wide peaks revealing that anthocyanin still exists inside maltodextrin particles. The FT‐IR spectrum had oscillations of characteristic groups of anthocyanin structure. Marshmallow samples added 5% anthocyanin powder gained high acceptability of appearance and maintained the scavenging capacity (DPPH) with an IC50 value of 7368.31 ppm after a month of storage.
Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control
Authors: Unverdi Aysegul , Erol Hilal Basak , Kaskatepe Banu , Babacan Orkun 
Published: January 2024

AbstractSalmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacteriophages were isolated from chicken feces. Two of the isolated phages (AUFM_Sc1 and AUFM_Sc3) were chosen for their characterization due to their broader host range. Based on morphological and genomic analysis, AUFM_Sc1 was identified to be close to similar Enterobacteria spp. CC31 (Myoviridae) and AUFM_Sc3 was identified to be close to Salmonella phage vB_Sen_I1 (Demerecviridae (formerly Siphoviridae)). Although these phages have shown promise for use in phage therapy applications for chickens, further studies are needed on their suitability. When a cocktail of these phages (AUFM_Sc1 + AUFM_Sc3) and nisin combination was applied on chicken breast meat, it was determined that it was effective against Salmonella contamination and while a good inhibitory effect was observed on the food, especially during the first 48 h, the effect decreased later, but the bacterial concentration was still low compared to the control group. Therefore, it is considered that the combination of AUFM_Sc1 + AUFM_Sc3 + nisin can be used as a food preservative against Salmonella.
Antioxidant, antidiabetic effects and polyphenolic contents of propolis from Siirt, Turkey
Authors: Ören Eda , Tuncay Salih , Toprak Yunus Emre , Fırat Muhammet , Toptancı İsra , Karasakal Ömer Faruk , Işık Mesut , Karahan Mesut 
Published: January 2024

AbstractPropolis, a natural product collected by honeybees from various plant sources, has gained significant attention due to its diverse bioactive compounds and potential therapeutic properties. To further explore its contents and biological activities, this study aimed to analyze the phenolic compounds in Siirt propolis extracts obtained using different solvents, namely ethanol, water, and ethanol‐water mixtures. The primary objective of this research was to investigate the phenolic profile, as well as the antidiabetic and antioxidant activities of the propolis extracts. Chemical profiling of extracts was performed using LC–MS/MS. The antioxidant potential of the propolis extracts was evaluated through free radical scavenging methods, including DPPH and ABTS assays. As a result of these analyses, propolis extracts showed moderate radical scavenging potential with 13.86%–35.72% for DPPH and 33.62%–62.50% for ABTS at a concentration of 30 μg mL−1, respectively. This radical scavenging potential of the extracts sheds light on its ability to combat oxidative stress, which is implicated in the development of diabetes, and its potential effects on cellular health. Additionally, the study assessed the antidiabetic properties of the propolis extracts by examining their inhibition effects on α‐amylase and α‐glycosidase enzymes. Extracts with high phenolic content showed a high inhibitory effect against α‐glucosidase with an IC50 of 5.72 ± 0.83 μg mL−1. This research provided significant findings regarding the potential use of propolis in the treatment of diabetes and related metabolic disorders.
A multi‐method approach for the identification and quantification of water balance components of two lentic small water bodies
Authors: Loose Lukas P. , Lange Jens , Budde Aaron , Herbstritt Barbara , Fohrer Nicola , Ulrich Uta 
Published: January 2024

AbstractThe protection of the globally widespread lentic small water bodies (LSWB) must be based on detailed knowledge about their hydrological connectivity and water balance. The study aimed to identify and quantify water balance components as well as surface‐groundwater interaction of two LSWB in a characteristic lowland region with a combination of different methods. This includes the collection of hydrological data and the use of bromide and water stable isotopes (δ2H and δ18O) as tracers. With their help, mixing models were established, and daily water balances were assessed. The results show a strong bidirectional interaction of both LSWB systems with shallow groundwater. Bromide and stable isotope tracers allowed for the identification of the most relevant in‐ and outflow sources and pathways. Thereby, isotope data revealed isotopic enrichment typical for open‐water bodies and only minor precipitation inputs mainly relevant at the end of the dry season. Water balance calculations suggested accentuated seasonal dynamics that were strongly influenced by shallow groundwater, which represented large inputs into both LSWB. By that, different phases could be identified, with high inflow rates in winter and spring and decreasing fluxes in summer. In one LSWB, a drainage system was found to have a major impact next to the shallow groundwater interaction. The findings of this research provide detailed insights into the influence and importance of shallow groundwater for LSWB in lowland regions. This impacts the diffuse input of agricultural pollutants into these ecologically important landscape features.
Spatiotemporal distribution and influencing factors of impervious surface evaporation in the Baiyangdian catchment from 1980 to 2020
Authors: Liu Xiaoyu , Mo Xingguo , Liu Suxia , Hu Shi 
Published: January 2024

AbstractEvaporation from impervious surfaces plays a vital role in the catchment water cycle. Exploring the spatiotemporal variation patterns and influencing mechanisms of impervious surface evaporation at the catchment scale can improve the understanding and evaluation of the evaporation process. This study downloaded 0.5 m resolution images of the Baiyangdian catchment (BYD) from Google Earth and used deep learning to identify impervious surfaces. This was used to revise impervious surfaces of the China land cover dataset in 1985 and 1990–2020. Potential evaporation (PET) from three types of impervious surfaces (roofs, ground affected or not affected by the building height) was calculated by modifying the parameters of the Penman–Monteith equation, and daily precipitation and water‐storage capacity of impervious surfaces were taken into account to estimate impervious surface evaporation (E). The results showed that E values of the three types of impervious surfaces were between 72.1 and 178.2 mm/year and all exhibited the spatial distribution of high in the northwest and low in the southeast of the BYD in 1980–2020. Compared with that in 1980, in 2020, the cumulative evaporation (EAP) increased by 134.4%. The ratio of EAP to cumulative precipitation ranged from 3.0% to 6.9%, increasing significantly in a fluctuating manner. The increments in precipitation days and impervious surface area played a major role in the increase of EAP, and the decrease in precipitation was the fundamental reason for the increase in the proportion of impervious surface evaporation and water resource pressure in the BYD. Excluding the continuous evaporation from the remaining water can likely lead to underestimating the impervious surface evaporation. This study provides an efficient and reasonable novel approach for calculating impervious surface evaporation in long series and large‐scale catchments.
Isotopic and hydrochemical insights into the groundwater characteristics along an arid to semi‐humid climate gradient in China
Authors: Chen Peiyuan , Ma Jinzhu , Ma Xiaoyi , Zhou Ying , Duan Zhihua , Wang Yueyue , Li Jisheng 
Published: January 2024

AbstractIn arid to semi‐arid regions, groundwater is a critical water resource heavily relied upon, with the recharge sources and patterns being predominantly shaped by climate change and regional disparities. To compare the characteristics of groundwater in the endorheic and exorheic river basins with the climate transition zone of Gansu Province, this study uses isotopic hydrochemical analyses. This study summarizes the differences in regional groundwater recharge and evolutionary patterns. The results shows that the distribution patterns of precipitation isotopes in endorheic and exorheic river basins are opposite to those of groundwater isotopes. Specifically, the precipitation in the endorheic areas is more depleted in heavy isotopes, whereas the groundwater is more enriched. Both endorheic areas and exorheic areas exhibit similar characteristics of groundwater hydrochemical evolution, evolving from low‐mineralization Mg2+HC recharge water to Na+Cl− type water with saline characteristics. The former is primarily replenished by surface water, whereas the latter is primarily replenished by precipitation. Variations in recharge patterns along with the differences in climatic conditions lead to distinct groundwater conditions in the two regions.
Interannual dynamics and controlling factors of the ratio of actual to potential evapotranspiration across typical ecosystems within the Heihe River Basin
Authors: Li Siying , Zhang Dapeng , Xing Yuhua , Zhang Chunlai , Wang Zhen‐ting , Ma Rui , He Kejian , Wang Pei 
Published: January 2024

AbstractThe ratio of actual to potential evapotranspiration (AET/PET) represents the water supply of terrestrial ecosystems and can be used to assess the water stress level of plants or ecosystems. This study quantified the control of meteorological, hydrological, and biological factors on AET/PET variations using variance partitioning analysis and path analysis. A soil–plant–atmosphere continuum model was validated and used to simulate AET and PET, as well as the interannual dynamics of AET/PET in typical ecosystems in the upper, middle, and downstream reaches of the Heihe River Basin from 2014 to 2016. The good agreement between measurements and simulations indicates that the model accurately represents the water/heat transfer process in various ecosystems within the Heihe River Basin. The study found that the alpine meadow ecosystem had the highest annual average AET/PET (0.89 ± 0.12), followed by the Populus euphratica ecosystem (0.72 ± 0.17) and cropland ecosystem (0.65 ± 0.15). AET/PET variation characteristics were comparable across the three ecosystems, showing apparent seasonality and interannual fluctuations. Plant transpiration stress was found to be a dominant indicator of ecosystem water availability. Changes in AET/PET were attributed to diverse environmental conditions in the three distinct ecosystems. While the relationship between soil water (SW) content and AET/PET weakened in cropland ecosystems with low water stress, the study demonstrates that SW content remains the most important factor governing AET/PET. The leaf are index and vapour pressure deficiency had significant indirect effects on AET/PET by influencing SW content. Overall, this study provides scientific support for optimizing water resource management in the Heihe River Basin by revealing the interannual variations and controlling factors of AET/PET in typical ecosystems.
Groundwater levels, climate and anthropogenic factors affect the hydrology and water quality of an intermittent and a regulated subtropical stream
Authors: Kim Donghwan , Lu Jing , Roberts Melanie E. , Roberts David T. , Orr David N. , Hamilton David P. 
Published: January 2024

AbstractStream hydrology and water quality are highly interconnected and impacted by climate, land use and geology. We examined this connection using monitoring data from 2000 to 2019 for two streams with contrasting hydrological regimes—intermittent and regulated perennial—in subtropical Queensland, Australia. Our main objective was to evaluate relationships between groundwater levels, climate and flow regulation on the hydrology and water quality of an intermittent and a regulated subtropical stream. In intermittently flowing Lockyer Creek, flow was highly dependent on groundwater levels and occurred when the aquifer was recharged to elevations exceeding the upper 90‐percentile value. With 9.4% of the catchment area in irrigated horticulture, flow in Lockyer Creek was also likely to be reduced by drawdown of the aquifer for irrigation, with no flow for 30% to 81% of days over the observation period for stations in Lockyer Creek. In contrast, flow in the mid‐Brisbane River was continuous, regulated by discharge from a large upstream dam. Nutrient and suspended sediment concentrations in Lockyer Creek were generally higher than in the mid‐Brisbane River, likely associated with runoff from agricultural areas adjacent to the stream, while the upstream dam likely reduced the concentration and variability of nutrients and suspended sediment in the mid‐Brisbane River. During periods of low flow in the mid‐Brisbane River, longitudinal changes in nutrient and suspended sediment concentrations occurred, notably a significant decrease in total and dissolved inorganic nitrogen concentrations downstream (p < 0.05), indicating a possible effect of in‐stream algal uptake and denitrification. This study highlights the impact of human modifications on stream hydrology and water quality in the face of climate change. The findings can inform decision‐making on groundwater irrigation or dam release control for water security.
Anti‐Candida activity and in vitro toxicity screening of antifungals complexed with β‐cyclodextrin
Authors: Moraes Gustavo Simão , Tozetto Nathaly Mayer , Pedroso Thaynara Aparecida Alves , de Mattos Marcela Alves , Urban Amanda Migliorini , Paludo Katia Sabrina , dos Santos Fábio André , Neppelenbroek Karin Hermana , Urban Vanessa Migliorini 
Published: January 2024

AbstractThe emergence of resistant fungal species and the toxicity of currently available antifungal drugs are relevant issues that require special consideration. Cyclodextrins inclusion complexes could optimize the antimicrobial activity of such drugs and create a controlled release system with few side effects. This study aimed to assess the in vitro toxicity and antifungal effectiveness of nystatin (Nys) and chlorhexidine (Chx) complexed or not with β‐cyclodextrin (βCD). First, a drug toxicity screening was performed through the Artemia salina bioassay. Then, the minimum inhibitory concentrations (MICs) against Candida albicans were determined with the broth microdilution test. After MICs determination, the cytotoxicity of the drugs was evaluated through the methyl‐thiazolyl‐tetrazolium (MTT) and neutral red (NR) assays and through cell morphology analysis. The PROBIT analysis was used to determine the median lethal concentration (LC50), and the cell viability values were submitted to one‐way analysis of variance(ANOVA)/Tukey (α = 0.05). Overall, the βCD‐complexed antifungals were less toxic against A. salina than their raw forms, suggesting that inclusion complexes can reduce the toxicity of drugs. The MICs obtained were as follows: Nys 0.5 mg/L; Nys:βCD 4 mg/L; Chx 4 mg/L; and Chx:βCD 8 mg/L. Chx showed significant cytotoxicity (MTT: 12.9 ± 9.6%; NR: 10.6 ± 12.5%) and promoted important morphological changes. Cells exposed to the other drugs showed viability above 70% with no cellular damage. These results suggest that antifungals complexed with βCD might be a biocompatible option for the treatment of Candida‐related infections.
Atypical meningeal localization of classical hairy cell leukemia with an impressive response to rituximab and cladribine association. A case report and literature review
Authors: Claves Fabien , Carras Sylvain , Burroni Barbara , Maitre Elsa , Boutonnat Jean , Troussard Xavier , Molina Lysiane 
Published: January 2024

AbstractHairy cell leukemia (HCL) is a rare lymphoproliferative disorder classically presenting with cytopenia and recurrent infections but atypical manifestations such as bone lesions, skin lesions and effusion have been described.We report here an unusual meningeal localization in a 33 years old man who presented with headache, hand paresthesia and visual symptoms. Brain magnetic resonance imaging revealed an occipital meningeal lesion. Diagnostic explorations led to the diagnosis of classical HCL with meningeal localization. After treatment by cladribine and rituximab the patient rapidly improved and is still in complete remission 12 months after end of treatment.The literature review identified 9 other cases of HCL with central nervous system localization (CNS) presenting with brain parenchyma and/or meninges localization. Four out of 9 patients presented with hyperleukocytosis. Most patients experienced good responses with various treatments. Cladribine alone or with rituximab led to complete responses similar to our patient. In our patient, molecular biology revealed KLF2 mutations, which implication in the atypical localization could be suspected but would need dedicated studies.In conclusion, CNS localizations of HCL are rare but can be observed and treatment with cladribine alone or with rituximab appears as an effective strategy.