bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compromised Influenza Virus-Specific CD8+-T-Cell Memory in CD4+-T-Cell-Deficient Mice
Authors: Belz Gabrielle T. , Wodarz Dominik , Diaz Gabriela , Nowak Martin A. , Doherty Peter C. 
Published: November 2002

ABSTRACTThe primary influenza A virus-specific CD8+-T-cell responses measured by tetramer staining of spleen, lymph node, and bronchoalveolar lavage (BAL) lymphocyte populations were similar in magnitude for conventionalI-Ab+/+and CD4+-T-cell-deficientI-Ab−/−mice. Comparable levels of virus-specific cytotoxic-T-lymphocyte activity were detected in the inflammatory exudate recovered by BAL following challenge. However, both the size of the memory T-cell pool and the magnitude of the recall response in the lymphoid tissues (but not the BAL specimens) were significantly diminished in mice lacking the CD4+subset. Also, the rate of virus elimination from the infected respiratory tract slowed at low virus loads following challenge of naïve and previously immunizedI-Ab−/−mice. Thus, though the capacity to mediate the CD8+-T-cell effector function is broadly preserved in the absence of concurrent CD4+-T-cell help, both the maintenance and recall of memory are compromised and the clearance of residual virus is delayed. These findings are consistent with mathematical models that predict virus-host dynamics in this, and other, models of infection.
Involvement of Apolipoprotein E in the Hematogenous Route of Herpes Simplex Virus Type 1 to the Central Nervous System
Authors: Burgos Javier S. , Ramirez Carlos , Sastre Isabel , Bullido María J. , Valdivieso Fernando 
Published: November 2002

ABSTRACTApolipoprotein E (ApoE), a constituent of the lipoproteins, may be relevant in herpes simplex virus type 1 (HSV-1) infection of the central nervous system (CNS), since HSV-1 binds to human serum ApoE lipoproteins. This study demonstrates the involvement of ApoE in the hematogenous route of HSV-1 to the CNS.
Inhibition of Hepatitis Delta Virus RNA Editing by Short Inhibitory RNA-Mediated Knockdown of ADAR1 but Not ADAR2 Expression
Authors: Jayan Geetha C. , Casey John L. 
Published: November 2002

ABSTRACT
Hepatitis delta virus (HDV) requires host RNA editing at the viral RNA amber/W site. Of the two host genes responsible for RNA editing via deamination of adenosines in double-stranded RNAs, short inhibitory RNA-mediated knockdown of host ADAR1 expression but not that of ADAR2 led to decreased HDV amber/W editing and virus production. Despite substantial sequence and structural variation among the amber/W sites of the three HDV genotypes, ADAR1a was primarily responsible for editing all three. We conclude that ADAR1 is primarily responsible for editing HDV RNA at the amber/W site during HDV infection.
Diagnosis and treatment of viral encephalitis
Authors: Chaudhuri A , Kennedy P G E 
Published: November 2002

Abstract
Acute encephalitis constitutes a medical emergency. In most cases, the presence of focal neurological signs and focal seizures will distinguish encephalitis from encephalopathy. Acute disseminated encephalomyelitis is a non-infective inflammatory encephalitis that may require to be treated with steroids. Acute infective encephalitis is usually viral. Herpes simplex encephalitis (HSE) is the commonest sporadic acute viral encephalitis in the Western world. Magnetic resonance imaging of brain is the investigation of choice in HSE and the diagnosis may be confirmed by the polymerase chain reaction test for the virus in the cerebrospinal fluid. In this article, we review the diagnosis, investigations, and management of acute encephalitis. With few exceptions (for example, aciclovir for HSE), no specific therapy is available for most forms of viral encephalitis. Mortality and morbidity may be high and long term sequelae are known among survivors. The emergence of unusual forms of zoonotic encephalitis has posed an important public health problem. Vaccination and vector control measures are useful preventive strategies in certain arboviral and zoonotic encephalitis. However, we need better antiviral therapy to meet the challenge of acute viral encephalitis more effectively.
Prostate cancer
Authors: Mazhar D , Waxman J 
Published: November 2002

AbstractIt is a paradigm in cancer treatment that early detection and treatment improves survival. However, although screening measures lead to a higher rate of detection, for small bulk localised prostate cancer it remains unclear whether early detection and early treatment will lead to an overall decrease in mortality. The management options include surveillance, radiotherapy, and radical prostatectomy but there is no evidence base to evaluate the benefits of each approach. Advanced prostate cancer is managed by hormonal therapy. There have been major changes in treatment over the last two decades with the use of more humane treatment and developments in both chemotherapy and radiation. In this article we review the natural history and management of prostate cancer.
Nocturnal leg cramps in older people
Authors: Butler J V , Mulkerrin E C , O’Keeffe S T 
Published: November 2002

AbstractNocturnal leg cramps are common in older people. Such cramps are associated with many common diseases and medications. Physiological methods may be useful for preventing cramps in some people, but there have been no controlled trials of these approaches. Quinine is moderately effective in preventing nocturnal leg cramps. However, there are concerns about the risk/benefit ratio with this drug. In patients with severe symptoms, a trial of 4–6 weeks’ treatment with quinine is probably still justified, but the efficacy of treatment should be monitored, for example using a sleep and cramp diary.
Current concepts for the management of systemic lupus erythematosus in adults: a therapeutic challenge
Authors: Ioannou Y , Isenberg D A 
Published: November 2002

Abstract
Systemic lupus erythematosus (SLE) is a chronic, autoimmune rheumatic disease with many clinical presentations typically affecting women of childbearing age. The successful therapy of SLE depends upon treating both symptoms and the underlying inflammation. Both non-pharmacological as well as pharmacological therapies are invariably required. Non-pharmacological therapy includes avoiding over-exposure to sunlight with the use of adequate sunscreen protection, avoiding “live” vaccination if on immunosuppressive agents, adherence to a diet low in saturated fat and high in fish oil, stress avoidance, and smoking cessation. Pharmacological measures revolve around four main classes of drugs: non-steroidal anti-inflammatory drugs, antimalarials, corticosteroids, and cytotoxic agents. Cyclophosphamide and azathioprine are the two most commonly used cytotoxic agents and these in combination with corticosteroids need to be employed early if there is major organ involvement to prevent or minimise irreversible damage. The potential side effects of corticosteroids and cytotoxic agents need constant consideration. The rapid developments in biotechnology of recent years may soon lead to new and more specific therapies for patients with SLE.
Surveillance of surgical training by detailed electronic registration of logical components
Authors: Tøttrup A 
Published: November 2002

Abstract

Objective
To obtain detailed information about the degree of surgical trainee supervision and delegation of procedures in a subspecialised department.

Design
Operative procedures and their logical components were recorded in a database constructed in Microsoft Access. Information about operating surgeon and assistants and their grade was registered prospectively over 12 months.

Subjects and methods
A total of 1250 intermediate or major procedures were performed by eight consultants, one staff specialist, four senior registrars, three specialist registrars, and five registrars.

Main measure
Number of components performed by surgeons in each grade and the degree of supervision and delegation.

Results
Eight hundred and eighty five of the operations were elective, while 365 were done as emergency procedures. Emergency procedures were far more often done by surgeons in training than by a staff surgeon, while the opposite was true for elective operations. Out of 323 elective operations done by surgeons in training, 189 were done under supervision (58.5%), while 119 out of 276 emergency operations done by surgeons in training were supervised (43.1%; p=0.0002). One hundred and twenty eight of 638 (20%) open abdominal operations were done by the most junior surgeons, and yet they closed 36% of all abdominal wounds. Although the most junior surgeons only served as operating surgeons in 39 of 334 bowel operations (12%), they constructed 24% of all stomas, and 20% of all anastomoses. Registrars and specialist registrars never constructed stomas or anastomoses without supervision.

Conclusion
Detailed information about individual and general training and supervision was achieved by a simple registration. Significant additional information was obtained about the extent of delegation of components compared with standard registration of operative procedures.
Does patient knowledge affect the colorectal cancer risk in ulcerative colitis?
Authors: Eaden J A , Abrams K , Mayberry J F 
Published: November 2002

Abstract

Objectives
Formal efforts to improve patient education are associated with fewer disease complications in a number of conditions. The possible relationship between knowledge about ulcerative colitis and its cancer risk, and the development of colorectal cancer using a previously developed and validated instrument—the Crohn’s and colitis knowledge (CCKNOW) score—were investigated.

Methods
The 24 item CCKNOW questionnaire was mailed to patients known to have developed colorectal cancer as a complication of ulcerative colitis (cases) and to colitics from the Leicestershire inflammatory bowel disease patient database who had not developed cancer (controls).

Results
The mean (SD) CCKNOW scores for cases was 8.21 (3.02) and for controls was 8.27 (4.3). These scores did not differ significantly between cases and controls (difference 0.06, 95% confidence interval (CI) −1.7 to 1.5, p=0.9). There were four times as many members of the National Association of Crohn’s and Colitis (NACC) in the control group compared with the cancer group and patients who are members of NACC achieve statistically significantly higher scores than non-members (11.6 v 7.8, p=0.05, 95% CI −0.1 to 7.6). However, after adjusting for NACC membership, the CCKNOW score did not appear to be associated with having developed cancer (odds ratio 1.04, 95% CI 0.92 to 1.18, p=0.5).

Conclusions
The CCKNOW scores were comparable in cases and controls. Thus, in a retrospective study, no evidence has been demonstrated of an association between patient knowledge and the risk of developing colorectal cancer in patients with ulcerative colitis. However, knowledge may have been increased in cases as a direct result of having had colorectal cancer as a complication of ulcerative colitis.
An unusual epistaxis
Authors: Saunders S , Young C 
Published: November 2002

Abstract
The case of a man who presented complaining of epistaxis is reported. He had coarctation repair 18 years previously. Subsequent investigation revealed an aortobronchial fistula resulting from false aneurysm formation distal to the original vessel anastamosis. This was repaired at surgery, the patient suffering a minor stroke, before rehabilitation and good recovery.
Bronchiolitis obliterans and organising pneumonia caused by carbamazepine and mimicking community acquired pneumonia
Authors: Banka R , Ward M J 
Published: November 2002

Abstract
Bronchiolitis obliterans and organising pneumonia (BOOP) presents with fever, dyspnoea, and other features that may be mistaken for pneumonia. Treatment is, however, very different, requiring corticosteroids.
A man was admitted as an emergency with fever, dyspnoea, and non-productive cough. The chest radiograph showed consolidation which, despite antibiotics, progressed to become bilateral. BOOP was considered, and confirmed by transbronchial biopsy. The response to oral prednisolone was rapid with complete resolution of symptoms and radiographic consolidation within three weeks. The cause of BOOP is often never found; it is believed, however, that in this instance it occurred as a result of carbamazepine therapy started seven weeks earlier.
Possible functional regression of insulinoma with prolonged octreotide
Authors: Craig C E H , Gallen I W 
Published: November 2002

Abstract
A 75 year old woman was treated for over three years with the somatostatin analogue, octreotide for an insulinoma. She had presented in a hypoglycaemic coma. C-peptide and insulin concentrations were both raised and an area of increased vascularity within the pancreas was shown by angiography. No lesion was found at laparotomy and no resection was performed. After over three years of octreotide treatment it was withdrawn for a week. Her insulin and C-peptide concentrations were greatly reduced at this time and remained so.
How safe is blood sampling? Anterior interosseus nerve injury by venepuncture
Authors: Zubairy A I 
Published: November 2002

Abstract
All invasive procedures carry some degree of risk of damage to the normal structures in the proximity of the region where the procedure is performed. The risk is, however, minimal for venous cannulation. A case is reported of an injury to the anterior interosseus nerve sustained during venepunture for routine blood sampling at the cubital fossa.
Animal Practices and the Racialization of Filipinas in Los Angeles
Authors: Lassiter Unna , Griffith Marcie , Wolch Jennifer 
Published: November 2002

AbstractMany factors contribute to the racialization of minority groups in the United States. Some individual characteristics, such as skin color or phenotype, are an obvious holdover from colonial times. Cultural differences in representational practices, customs and rituals, and belief systems are now more significant in racialization. Although not typically a focus of academic scrutiny, some of these differences involve contrasts in nature-society relations, and more specifically, nonhuman animal-society relations. In order to examine the relationship between culturally based animal practices and racialization, we organized and conducted a focus group consisting of low-income inner city Filipinas living in Los Angeles, California. Analysis of focus group data reveal that Filipinos in southern California are subject to racialization by Anglos because of their culturally based animal practices, in particular the traditional Filipino practice of treating dogs as food animals. The experience of racialization appeared to engender cultural relativism and tolerance toward the animal practices of other non-Anglo groups.
Ethnic Variations in Pet Attachment among Students at an American School of Veterinary Medicine
Authors: Brown Sue-Ellen 
Published: November 2002

AbstractThis study explores ethnic variations in animal companion ("pet") attachment among 133 students enrolled in a school of veterinary medicine. The 57 White and 76 African American participants completed surveys that included background information, several questions about their animal companions, and a pet attachment questionnaire (PAQ).White students had significantly higher PAQ scores than did African American students (p<.001). White students also had significantly more pets (M =4.05 vs. 2.18, p<.001) and more kinds of pets (M =2.30 vs. 1.57, p<.001) and were more likely to allow pets to sleep on their beds (70% vs. 53%, p<.05). Although keeping pets is a universal cultural phenomenon, how that attachment is expressed may vary from culture-to-culture. This study explores possible explanations and implications for these variations.
Human-to-Animal Similarity and Participant Mood Influence Punishment Recommendations for Animal Abusers
Authors: Waerstad Jon , Hobbins Thea , Wirrel Joanne , Hunstone Mathew , Foy Emma , Allen Michael , Wikner Britt 
Published: November 2002

AbstractStudies of observer responses to human-to-human abuse have found that both an observer's mood and the similarity of the victim to the observer affect the observer's desire to help the victim and punish the offender. The present study examined the extent to which similarity and mood also shape observer responses to human-to-animal abuse.We first manipulated participants' mood by giving non-contingent feedback on a hidden word task (positive, negative, or no feedback). Participants then read a scenario describing an instance of animal abuse (using four different specific kinds of animals and six general species categories). Results showed that participants in a better mood recommended harsher punishment for the offender.They also recommended harsher punishment for the abuse of animals more similar to humans. Similarity and mood interacted on fine recommendationsmdash;better mood accentuated the similarity effect. Empathy for an animal positively correlated with punishment recommendations for the offender. The study discusses directions for future research and theory development.
The Wild Side of Animal Domestication
Authors: Russell Nerissa 
Published: November 2002

AbstractThis paper examines not the process but the concept of nonhuman animal domestication. Domestication involves both biological and cultural components. Creating a category of domestic animals means constructing and crossing the boundaries between human and animal, culture and nature. The concept of domestication thus structures the thinking both of researchers in the present and of domesticators and herders in the past. Some have argued for abandoning the notion of domestication in favor of a continuum of human-nonhuman animal relationships. Although many human-animal relationships cannot be neatly pigeonholed as wild or domestic, this paper contends that the concept of domestication retains its utility.There is a critical distinction between animals as a resource and animals as property. Domestication itself had profound consequences for the societies and worldview of the domesticators and their descendents. In addition to the material effects of animal wealth, domestic animals provide both a rich source of metaphor and a model of domination that can be extended to humans.
Marxism and the Underdog
Authors: Perlo Katherine 
Published: November 2002

AbstractMarxism has defined its key values in opposition to animals other than human in order to promote the interests of the most downtrodden human beings. Although it has characterized itself as a scientific historical and economic theory, sympathy for human suffering has provided its most powerful motivation as a political force. This capacity for sympathy, causing in modern times an extension of Marxist concerns beyond "class" in the original sense, is beginning to accommodate animals as are the theoretical concepts of alienation, surplus value, and historical materialism. Marxism's inconsistencies are being resolved in favor of the side that, for human as well as animal benefit, favors individual sentience and other pro-animal values. So, in a truly dialectical progression, the same quality of sympathy that at first caused Marxism to denigrate animals is now coming out in their support.
Toward an Ideal Synthesis of Oligonucleotides: Development of a Novel Phosphoramidite Method with High Capability
Authors: Hayakawa Yoshihiro 
Published: November 2002

Abstract
Several novel methods for reliably carrying out the oligonucleotide synthesis via the phosphoramidite strategy have been developed. The first is the method using allyloxycarbonyl and allyl groups for the protection of nucleoside bases and internucleotide linkages, respectively. These protectors can be cleanly removed under almost neutral conditions with a mixture of an organic Pd(0) complex such as Pd[P(C6H5)3]4 or Pd2[(C6H5CH=CH)2CO]3·CHCl3, and P(C6H5)3 in the presence of a nucleophile such as butylammonium formate, diethylammonium hydrogencarbonate, diethylammonium carbonate, or dimedone. A method for the facile oxidation of nucleoside phosphites to phosphates has been developed; this is performed under non-aqueous and non-basic conditions using 2-butanone peroxide in dichloromethane, t-butyl hydroperoxide in toluene, or bis(trimethylsilyl) peroxide in dichloromethane. The combined use of the N-allyloxycarbonyl/P–O-allyl-protected method and anhydrous/non-basic oxidation is extremely useful for the synthesis of nucleotide derivatives sensitive to moisture and/or bases, producing them in higher yield and at a greater level of purity than the existing methods using acyl or aroyl and cyanoethyl groups for the protection of nucleoside bases and internucleotide linkages, respectively, and iodine in aqueous pyridine for the oxidation. Further, acid/azole complexes serving as highly useful activators of phosphoramidites have been invented. The reagents include imidazolium triflate, N-(phenyl)imidazolium triflate, benzimidazolium triflate, and N-(methyl)benzimidazolium triflate, which allow higher-yield synthesis of oligonucleotides than conventionally employed 1H-tetrazole and related reagents. For example, synthesis of oligoribonucleotides via the allyloxycarbonyl/allyl-protected strategy has been carried out more efficiently using N-(phenyl)imidazolium triflate as a promoter than 5-(ethylthio)-1H-tetrazole, which has been found to be the most effective promoter among those currently available for oligoribonucleotide synthesis. Further, imidazolium triflate has opened the door to the extremely convenient and efficient synthesis of oligodeoxyribonucleotides without nucleoside-base protection. This method is obviously advantageous over N-protected methods because it eliminates tedious operations and the use of superfluous reagents required for the introduction and removal of the N-protecting group; risks of decomposition and loss of product by protection and deprotection are also avoided. Facile liquid-phase preparation of short oligomers has also been developed; this is successfully accomplished using stoichiometric amounts of building blocks by the aid of an equimolar amount of azolium reagent or a catalytic amount of 5-(p-nitrophenyl)-1H-tetrazole. These two methods are beneficial from an economical point of view, particularly in large-scale synthesis.
Unusual Fluorescent Properties of Novel Fluorophores, 6-Aryl-3,4-diphenyl-α-pyrone Derivatives
Authors: Hirano Keisuke , Minakata Satoshi , Komatsu Mitsuo 
Published: November 2002

Abstract
Novel fluorophores, 6-aryl-3,4-diphenyl-α-pyrones, were synthesized and their spectroscopic properties investigated in the form of evaporated films on plain glass slides, as well as in the solid and solution states. An electron-donating aryl group on the 6-position of the pyrones causes a red-shift in the absorption and fluorescent maxima. In the solid states, they show intense blue-to-orange fluorescence, but not in solution. This unusual fluorescent property is caused by fixing the 6-aryl group of the pyrones, and is the result of molecular packing. These interactions induce a pathway for radiative decay, which is associated with intense fluorescence emission only in the solid state.