bluuebunny/crossref_metadata_2025_split huggingface dataset:
From iRBD to Parkinson’s disease: Tracking Glymphatic Dysfunction Using Automated DTI-ALPS Analysis
Authors: Marecek S , Rottova V , Nepozitek J , Krajca T , Krupicka R , Keller J , Zogala D , Trnka J , Sonka K , Ruzicka E , Dusek P 
Published: January 2025

AbstractDiffusion tensor image analysis along the perivascular space (DTI-ALPS) is a non-invasive marker of glymphatic function that typically relies on manual region of interest (ROI) placement. This study compared glymphatic function in treatment-naïve, de novo diagnosed patients with Parkinson’s disease (PD), patients with isolated REM behavior disorder (iRBD), and healthy controls using both manual and automatic DTI-ALPS methods. ALPS scores were analyzed bilaterally and correlated with clinical severity (MDS-UPDRS) and nigrostriatal denervation (DAT-SPECT). The study included 79 PD patients (60±12 years), 57 iRBD patients (67±7 years), and 48 controls (62±10 years). ANCOVA revealed significant inter-group differences using both manual (p=0.018) and automatic (p=0.002) methods. Automatic analysis showed significantly lower ALPS scores in PD compared to controls (p=0.001) and iRBD (p=0.009). ALPS scores correlated with symptom severity and nigrostriatal degeneration. These findings highlight early glymphatic dysfunction in PD and demonstrate the reliability of the automatic DTI-ALPS method.
Effect of moderate altitude with and without nocturnal oxygen therapy on the next-day cerebrovascular function in patients with chronic obstructive pulmonary disease – A randomized, cross-over trial at 2048 m
Authors: Gilliand Dominic , Latshang Tsogyal D. , Aeschbacher Sayaka S. , Huber Fabienne , Flueck Deborah , Lichtblau Mona , Ulrich Stefanie , Hasler Elisabeth D. , Scheiwiller Philipp M. , Müller Julian , Ulrich Silvia , Bloch Konrad E. , Furian Michael 
Published: January 2025

AbstractBackgroundThis study investigated whether nocturnal oxygen therapy (NOT) improves next-day cerebrovascular function in lowlanders with COPD staying at moderate altitude.MethodsThis randomized, placebo-controlled crossover trial was performed in stable patients with moderate to severe COPD (FEV1/FVC <0.7 and FEV130-80%predicted), living <800m and pulse oximetry (SpO2) ≥92%. Patients underwent assessments at 490m and during 2 stays of 2 days at 2048m while NOT or placebo (each at 3L min−1through nasal cannula) were applied according to a randomized cross-over design. At both altitudes, SpO2, cerebral tissue oxygenation (CTO, near-infrared spectroscopy), mean arterial blood pressure (MAP, finger plethysmography) and middle cerebral artery systolic peak blood flow velocity (sMCAv, transcranial Doppler ultrasound) were assessed while patients were(0)quietly breathing FiO20.21;(i)quietly breathing FiO21.0,(ii)voluntarily hyperventilating,(iii)voluntarily hyperventilating under FiO21.0, and(iv)during head-up tilting. Indices of cerebrovascular responsiveness to changes in blood gases and blood pressure were computed.ResultsA total of 18 patients (8 women aged mean±SD 65±5y, FEV154.7±13.9%predicted) were analyzed. At 2048m under placebo, patients became hypoxemic, mean±SE SpO290.3±0.4% vs. 93.7±0.4% at 490m, while MAP, CTO and sMCAv remained unchanged compared to 490m. All ventilatory maneuvers at 2048 m induced greater increases in SpO2compared to 490m while changes in MAP, CTO and sMCAv were similar. Head-up tilting induced a similar blood pressure fall at 2048m compared to 490m, whereas cerebral blood flow velocity changed less in response to systemic hypotension (mean±SE ΔsMCAv/ΔMAP 0.9±0.3 vs. 2.3±0.4cm s−1mmHg−1) at 2048m. No alteration in cerebrovascular function as a treatment effect of NOT was observed in either maneuver.ConclusionThis randomized clinical trial in moderate-to-severe COPD patients ascending to 2048m showed that moderate daytime systemic hypoxemia does not translate to cerebral hypoxia nor cerebrovascular autoregulatory impairments while at rest or under ventilatory or orthostatic challenges.
Integrating transcriptomic data with metabolic model unravels the electron transfer mechanisms ofMethanosarcina barkeri
Authors: Tang Wentao , Lin Sen , Deng Yangfan , Guo Gang , Chen Guanghao , Hao Tianwei 
Published: January 2025

AbstractMethanogenic archaea, particularlyMethanosarcina, are pivotal to the global carbon cycle and renewable energy production due to their versatile metabolic capabilities. Although transcriptomic analysis is widely employed to identify key genes and pathways inMethanosarcinaunder various methanogenic conditions—including the emerging direct interspecies electron transfer (DIET)-based methanogenesis—the weak correlation between gene expression levels and protein abundances poses challenges for interpreting transcriptomic data. To address this, we integrated transcriptomic data into a metabolic model ofMethanosarcina barkerifor the first time, enabling more refined predictions and enhancing the interpretability of transcriptomic insights. This novel integrated model was subsequently utilized to simulate aceticlastic, hydrogenotrophic, and DIET-based methanogenesis. The results revealed that previous assumptions failed to account for the role of the CO₂ reduction pathway in aceticlastic methanogenesis. The model also successfully captured key transcriptomic features of DIET-based methanogenesis, clarifying the functional roles of crucial enzymes like the F420H2dehydrogenase Fpo and the transmembrane hydrogenase Vht in electron transfer. This integrative approach provided a deeper understanding of electron transfer mechanisms inM. barkeriand offered valuable insights for advancing methanogen-based biotechnologies. Moreover, the study critically evaluated the relationship between gene expression and metabolic flux, establishing a practical framework for deriving meaningful insights from the growing volume of transcriptomic data.
The influence of fetal sex on maternal blood pressure in pregnancy
Authors: Decina Caitlin S. , Beaumont Robin N. , Juodakis Julius , Warrington Nicole M. , Patel Kashyap A. , Njølstad Pål R. , Johansson Stefan , Hattersley Andrew T. , Jacobsson Bo , Lowe William L. , Evans David M. , Freathy Rachel M. 
Published: January 2025

AbstractBackgroundPregnancy with a male fetus carries a higher risk of term pre-eclampsia than pregnancy with a female fetus. Based on evidence that maternal blood pressure (BP) may be raised in pregnancies with Beckwith-Wiedemann syndrome (fetal overgrowth), a possible contributing factor to the association between male sex and term pre-eclampsia is that males grow faster, reaching ∼130 g higher birth weight, on average, than females. The association between fetal sex and maternal BP in healthy pregnancies is not known. We hypothesized that male sex would be associated with higher maternal BP in healthy pregnancies, and that this association would be explained by birth weight differences between males and females.Methods and findingsWe tested the association between fetal sex and maternal systolic (SBP) and diastolic blood pressure (DBP), measured at ∼28 weeks of gestation, in a meta-analysis of five different cohorts of mother-child pairs (n up to 109,842). Maternal BP was analyzed as both a continuous and dichotomized (high BP: yes or no) outcome. Linear regression models were constructed with and without adjustment for birth weight to assess whether any difference in maternal BP was explained by the difference in birth weight between male and female babies. Lastly, we constructed a fetal genetic score for birth weight using 186 own-birth-weight-associated single-nucleotide polymorphisms (SNPs) to test whether birth-weight-raising-alleles in the fetus were associated with maternal BP in pregnancy (n up to 32,232). Both maternal SBP and DBP were higher in pregnancy when carrying a male fetus compared to a female fetus (mean difference 0.35 mmHg [95%CI: 0.15-0.55] and 0.35 mmHg [95%CI: 0.21-0.49], for SBP and DBP, respectively). An independent effect of fetal sex remained when including birth weight but attenuated slightly (0.22 mmHg [95%CI: 0.02-0.42] and 0.31 mmHg [95%CI: 0.17-0.45], for SBP and DBP respectively). A positive effect estimate was found for odds of experiencing high maternal BP given pregnancy with a male fetus, but confidence intervals were wide (OR 1.05 [95%CI: 0.98-1.12]). No evidence for an association was found between a fetal birth weight genetic score and SBP or DBP when conditioned on maternal genotype.ConclusionsWe found strong evidence to support a small effect of male fetal sex on higher maternal BP in pregnancy and that larger fetal size at birth does not contribute to a substantial part of this association. Our findings do not indicate a difference in maternal BP that would warrant changes to routine monitoring in clinical practice but do suggest that male sex may be a contributing risk factor for BP-related complications.
Receptor Allostery Promotes Context-Dependent Sonic Hedgehog Signaling During Embryonic Development
Authors: Ansari Shariq S. , Dillard Miriam E. , Ghonim Mohamed , Zhang Yan , Stewart Daniel P. , Canac Robin , Moskowitz Ivan P. , Wright William C. , Daly Christina A. , Pruett-Miller Shondra M. , Steinberg Jeffrey , Wang Yong-Dong , Chen Taosheng , Thomas Paul G. , Bridges James P. , Ogden Stacey K. 
Published: January 2025

AbstractSonic Hedgehog (SHH) signaling functions in temporal- and context-dependent manners to pattern diverse tissues during embryogenesis. The signal transducer Smoothened (SMO) is activated by sterols, oxysterols, and arachidonic acid (AA) through binding pockets in its extracellular cysteine-rich domain (CRD) and 7-transmembrane (7TM) bundle.In vitroanalyses suggest SMO signaling is allosterically enhanced by combinatorial ligand binding to these pockets butin vivoevidence of SMO allostery is lacking. Herein, we map an AA binding pocket at the top of the 7TM bundle and show that its disruption attenuates SHH and sterol-stimulated SMO induction. A knockin mouse model of compromised AA binding reveals that homozygous mutant mice are cyanotic, exhibit high perinatal lethality, and show congenital heart disease. Surviving mutants demonstrate pulmonary maldevelopment and fail to thrive. Neurodevelopment is unaltered in these mice, suggesting that context-dependent allosteric regulation of SMO signaling allows for precise tuning of pathway activity during cardiopulmonary development.
RNA modifications on Adenosine Regulate Macrophage Function
Authors: Griesche Valerie , Di Giorgio Salvatore , Brettschneider Julia , Tellioglu Irem , Kao Chih-Yuan , Lorenzo Jose Paulo , Pezzella Laura , Stoecklin Georg , Papavasiliou F. Nina 
Published: January 2025

AbstractMacrophages are important innate immune cells that respond rapidly to inflammatory cues and fulfill diverse functions in the activation, execution and resolution of immune and inflammatory responses. Currently, there is no comprehensive understanding of the combinatorial effect of mRNA modifications in a single cell type, the consequences for mRNA processing and metabolism, and how these changes affect cell physiology. We use the mouse macrophage line RAW264.7 (RAW) to systematically address the combinatorial effect of two major mRNA modifications during macrophage stimulation: adenosine to inosine deamination (A-to-I), and adenosine N6 methylation (m6A). At the functional level we found that both METTL3 (the m6A writer) and ADAR1 (the adenosine deaminase) are required for full activation of RAW macrophages (as determined by expression of surface markers) and for optimal phagocytosis (a key macrophage function). Using both short read and single molecule sequencing on RAW cells with genetic deletions of the m6A writer METTL3 and the A deaminase ADAR1, we found that loss of m6A affected A-to-I levels while m6A levels remained relatively stable. A detailed investigation of potential interactions between m6A and A-to-I at the single transcript level is ongoing.
Practice what you preach: the effects of mission alignment on organizational performance
Authors: MacKenzie William I. , Colazo Jorge A. , Scherer Robert F. 
Published: January 2025

PurposeAssociation to Advance Collegiate Schools of Business (AACSB) accreditation encourages business schools to exhibit alignment within their mission, strategies and outcomes to achieve success. The present study aims to explore the idea of mission alignment and how it may serve as an important moderator to the relationship between organizational resources and school performance as measured through business school rank.Design/methodology/approachOur study utilizes the AACSB International business school survey (BSQ) data to analyze the mission statements of accredited business schools and capture data on organizational resources. We also created an index of mission alignment to gauge congruency between the stated mission and strategic focus. Our performance measure was the U.S. News and World Report undergraduate business school programs ranking value.FindingsOur results show mission alignment on its own has little direct impact on organizational performance. However, when mission statement alignment and resource allocations are combined, they interact to influence organizational performance.Originality/valueOur research demonstrates that resource allocation decisions and mission alignment are two important attributes of an organization and that mission alignment has the potential to leverage an organization’s resources and capabilities to improve performance.
Empowering healthcare: the link of self-efficacy, employee engagement, and citizenship behaviors
Authors: Bardhan Shravana , Haque Shamima 
Published: January 2025

Purpose
This study investigates employee engagement within private healthcare settings, with a particular emphasis on the connection between occupational self-efficacy and organizational citizenship behaviors.

Design/methodology/approach
A mixed-methods approach was utilized for this research. Interviews were conducted with employees across different managerial levels to identify various employee engagement strategies implemented in eight private hospitals in West Bengal. Subsequently, a survey of 414 healthcare employees from these institutions was conducted using a structured questionnaire. The study employed Confirmatory Factor Analysis, linear regression, and mediation analysis using the Sobel test to explore the relationships between variables.

Findings
The study revealed a significant positive correlation among all variables. Employee engagement was identified as a mediating factor between occupational self-efficacy and organizational citizenship behavior.

Research limitations/implications
This research is focused exclusively on the healthcare sector, with no data collected from other sectors, which may limit the generalizability of the findings. The sample size and geographic scope were restricted, which may not capture the full diversity of perspectives within the healthcare sector.

Practical implications
This research indicates that OSE promotes OCB, with this effect amplified when mediated by EE. Thus, fostering EE can significantly contribute to achieving OCB, a crucial factor in the healthcare sector. Given these insights, public healthcare institutions, which often serve a larger and more diverse patient population, could benefit from adopting similar engagement-enhancing practices. In these high-pressure environments, where employees frequently need to exceed their formal role definitions, cultivating a culture of engagement becomes paramount. By implementing targeted EE initiatives, government hospitals can potentially improve OCB among their staff, leading to enhanced patient care and operational efficiency. Government healthcare HR professionals can enhance OSE through training, coaching, and talent recognition programs. Empowering employees via skill development and participative decision-making can boost OSE and its positive impact on OCB.

Originality/value
This study offers novel insights that can inform practices in both private and public healthcare sectors, representing a pioneering effort in the Indian context. By examining these dynamics, the research aims to contribute to the development of strategies that enhance employee engagement, ultimately leading to improved patient care that goes beyond standard job descriptions.
The impact of reverse jeonse incidents on the risk of nonreturn of security deposits: an empirical analysis of the South Korean housing market
Authors: Park Jinbaek , Park Kiduk 
Published: January 2025

Purpose
This study aims to analyze the impact of reverse jeonse on the risk of nonreturn of security deposits in the South Korean housing market. Reverse jeonse refers to a situation where the market price of a jeonse contract drops below the agreed price, which increases the likelihood of landlords being unable to return deposits.

Design/methodology/approach
The analysis uses data from 17 South Korean regions, covering apartments and multifamily dwellings from 2017 to 2022. Key indicators of nonreturn risk include leasehold registration orders, court auctions and jeonse deposit guarantee incidents. A panel regression model is applied to quantify the correlation between reverse jeonse incidents and the increased risk of nonreturn.

Findings
The study finds that an increase in reverse jeonse incidents correlates with a higher frequency of leasehold registration orders, court auctions and deposit guarantee cases, particularly after 2021. Economic disruptions and policy changes exacerbated landlords’ financial burdens, highlighting the need for interventions to stabilize the jeonse market.

Practical implications
The findings suggest strengthening jeonse deposit guarantee schemes and refining legal procedures to mitigate the risk of nonreturn. Policy recommendations include targeted interventions to stabilize the market and protect tenant deposits.

Originality/value
This study fills a gap in existing literature by empirically linking reverse jeonse to the nonreturn of deposits and offering data-driven policy recommendations to ensure market stability and tenant protection.
External driver of corporate green innovation: Does customer environmental concern matter?
Authors: Jia Zhiqiang , Li Weian , Xu Jian 
Published: January 2025

PurposeThe purpose of this study is to examine the impact of customers' environmental concern on corporate green innovation and its underlying mechanisms.Design/methodology/approachThis study empirically examines the impact of customer environmental concern on corporate green innovation using 967 company-customer-year observations of Chinese A-share listed companies over the period 2012–2022.FindingsThe empirical results show that customer environmental concern significantly enhances corporate green innovation. Furthermore, executive environmental awareness and research and development (R&D) investment play a partial mediating role in this relationship. The heterogeneity analysis reveals that state-owned customers, customers located in the same province with the corporate and the intellectual property model cities contribute to strengthening this relationship. Moreover, corporate performance analysis shows that customer environmental concern can significantly increase corporate financial performance and sustainable performance.Originality/valueThis study innovatively proposes a measure of customer environmental concern and examines its impact on corporate green innovation and its underlying mechanisms. In addition, this study also proposes some insights for policymakers.
Reusing the Ghoufi’s vernacular buildings within the context of sustainable conservation, an action put to the test
Authors: Bourayou Houda , Aichour Boudjemâa 
Published: January 2025

PurposeVernacular architectures are now seen as sustainable construction models for their quality and environmental solutions. This study examines Ghoufi’s Chaouie constructive culture, which adapted to harsh climates by optimizing resources and integrating vegetation, construction and animal husbandry. Yet, modernization efforts have often endangered this heritage through harmful rehabilitation. Our main goal is to preserve this resilient architecture through sustainable conservation that respects its cultural integrity.Design/methodology/approachAn experimental method, based on the principles of integrated conservation, was employed to evaluate the reuse and readaptation potential of vernacular buildings. The research involved an initial discovery phase to examine the building practices, environmental context and Chaouie cultural heritage. Evaluation is conducted through a pilot study, where we conduct a rehabilitation trial on a model house, followed by a public survey, to refine the experimentation and obtain reliable and quality results.FindingsThe study concludes with methodological guidelines for a sustainable conservation strategy for Ghoufi. The findings demonstrate the feasibility of reviving traditional techniques, leveraging local expertise and resources and promoting environmentally responsible practices in the conservation of vernacular architecture.Originality/valueThe study lies in its holistic, field-based approach to sustainable conservation, centered on Ghoufi’s cultural and environmental context. Unlike prior interventions, this research integrates traditional construction, Chaoui community values and ecotourism as drivers of sustainable territorial development. It demonstrates the feasibility of reusing vernacular structures through an inclusive, community-focused conservation strategy that preserves Ghoufi’s identity while supporting long-term sustainability.Highlights(1)The Algerian civil war, known in Algeria as the black decade, lasted from 1992 to 2002.(2)The name of the local population of the Aures region I, including Ghoufi.(3)Tourist expansion zone.(4)Permanent Plan for the Conservation and Enhancement of the Protected Sector [Author’s translation].(5)These are development and planning schemes in Algeria at the regional territorial level and at the level of the province (wilaya).(6)These are agencies supporting youth employment, micro-credits and support units for assisting young entrepreneurs in job creation.
How and when does enterprise social media usage threaten employees’ thriving at work? An affective perspective
Authors: Li Yi , Liu Renjing 
Published: January 2025

PurposeThe impact of enterprise social media (ESM) on employees is not always beneficial. The constant connectivity of ESM can trigger emotional issues, threatening employees' psychological states. This study aims to explore the potential risks of ESM usage on thriving at work by examining how ESM usage affects thriving and for whom this relationship is amplified.Design/methodology/approachDrawing on Affect Event Theory, this study proposes a research model for investigating the underlying mechanisms and boundary conditions of the negative impacts of different ESM usage patterns on employees' thriving at work. This study employed Smart-PLS 4.0 with structural equation modeling to analyze multi-wave survey data from 255 employees, testing the proposed model.FindingsThe results indicate the following: (1) ESM usage triggers employees' workplace fear of missing out (WFoMO), increasing their online vigilance and consequently hindering their thriving at work. (2) The impact of ESM usage on employees' WFoMO and thriving at work varies with their ambition levels, with high-ambition employees being more susceptible to negative effects and (3) Different types of ESM usage exert distinct effects on employees.Originality/valueThis study substantiates the potential negative impact of ESM usage on employees' thriving at work, contributing to the literature on the “dark side” of ESM usage and thriving at work. This study confirms the critical mediating role of emotion, offering a novel theoretical perspective on understanding the mechanisms linking ESM usage and its outcomes. Additionally, this study identifies the moderating role of employee ambition, complementing the boundary conditions of ESM usage.