bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of life and stigmatization in people with skin diseases in Europe: A large survey from the ‘burden of skin diseases’ EADV project
Authors: Gisondi Paolo , Puig Luis , Richard Marie Aleth , Paul Carle , Nijsten Tamar , Taieb Charles , Stratigos Alex , Trakatelli Myrto , Salavastru Carmen ,   the EADV Burden of Skin Diseases Project Team
Published: October 2023

AbstractBackgroundSeveral large studies on the burden of skin diseases have been performed in patients recruited in hospitals or clinical centres, thus missing people with skin diseases who do not undergo a clinical consultation.ObjectivesTo evaluate the burden of the most common dermatological diseases in adult patients across Europe, in terms of quality of life, work life, and stigmatization.MethodsPopulation‐based survey on a representative sample of the European general population aged 18 years or older. Participants who declared to have had one or more skin problem or disease during the previous 12 months completed the Dermatology Life Quality Index questionnaire, and answered questions regarding the impact of their skin disease on daily and work life, anxiety/depression, and stigmatization.ResultsThe study population included 19,915 individuals, 44.7% of whom were men. Quality of life was particularly impaired in people with hidradenitis suppurativa (HS), and sexually transmitted diseases. About a half of participants with acne, alopecia, or chronic urticaria, and about 40% of those with atopic dermatitis (AD), skin cancers, or psoriasis reported a modest to extremely large effect of the disease on their quality of life. Overall, 88.1% of participants considered their skin disease as embarrassing in personal life and 83% in work life. About half of the respondents reported sleeping difficulty, feeling tired, and impact of the disease on taking care of themselves. Concerning stigmatization, 14.5% felt to have been rejected by others because of the skin disease, and 19.2% to have been looked at with disgust. Anxiety and depression were frequently reported by patients with all the diseases.ConclusionsSkin diseases may heavily affect patients' daily and work life, and cause feelings of stigmatization. An early intervention is needed to avoid consequences on the patients' life course.
Patient prioritisation of impact items to develop the patient‐reported impact of dermatological diseases (PRIDD) measure: European Delphi data
Authors: Trialonis‐Suthakharan N. , Pattinson R. , Tahmasebi Gandomkari N. , Austin J. , Janus C. , Courtier N. , Hewitt R. M. , Bundy C. , Augustin M. 
Published: October 2023

AbstractBackgroundThe Global Research on the Impact of Dermatological Diseases (GRIDD) project is developing a patient‐reported measure of the impact of dermatological disease on the patient's life called Patient Reported Impact of Dermatological Diseases (PRIDD). We developed a list of 263 potential impact items through a global qualitative interview study with 68 patients. We next conducted a Delphi study to seek consensus on which of these items to prioritize for inclusion in PRIDD. This study aims to explore patterns in demographic (e.g. country) and clinical variables (e.g. disease group) across the impacts ranked as most important to European dermatology patients.MethodsWe conducted a modified, two rounds Delphi study, testing the outcomes from the previous qualitative interview study. Adults (≥18 years) living with a dermatological disease were recruited through the International Alliance of Dermatology Patient Organizations' (GlobalSkin) membership network. The survey consisted of a demographic questionnaire and 263 impact items and was available in six languages. Quantitative data were collected using ranking scales and analysed against a priori consensus criteria. Qualitative data were collected using free‐text responses and a Framework Analysis was conducted. European data were obtained, and descriptive statistics, including multiple subgroup analyses, were performed.ResultsOut of 1154 participants, 441 Europeans representing 46 dermatological disease from 25 countries participated. The results produced a list of the top 20 impacts reported by European patients, with psychological impacts accounting for the greatest proportion.ConclusionThis study identified what patients consider to be the most important issues impacting their lives as a result of their dermatological disease. The data support previous evidence that patients experience profound psychological impacts and require psychological support. The findings can inform research, clinical practice and policy by indicating research questions and initiatives that are of most benefit to patients.
Polyubiquitin protein of Aedes aegypti as an interacting partner of dengue virus envelope protein
Authors: Yadav Karuna , Saurav Gunjan Kumar , Rana Vipin Singh , Rawat Nitish , Anjali  , Jamwal Rohit , Singh Om Prakash , Bandyopadhyay Anannya , Rajagopal Raman 
Published: October 2023

AbstractDengue virus (DENV) is an arbovirus that comprises four antigenically different serotypes. Aedes aegypti (Diptera: Culicidae) acts as the principal vector for DENV transmission, and vector control is crucial for dengue fever epidemic management. To design effective vector control strategies, a comprehensive understanding of the insect vector and virus interaction is required. Female Ae. aegypti ingests DENV during the acquisition of a blood meal from an infected human. DENV enters the insect midgut, replicates inside it and reaches the salivary gland for transmitting DENV to healthy humans during the subsequent feeding cycles. DENV must interact with the proteins present in the midgut and salivary glands to gain entry and accomplish successful replication and transmission. Ae. aegypti midgut cDNA library was prepared, and yeast two‐hybrid screening was performed against the envelope protein domain III (EDIII) protein of DENV‐2. The polyubiquitin protein was selected from the various candidate proteins for subsequent analysis. Polyubiquitin gene was amplified, and the protein was purified in a heterologous expression system for in vitro interaction studies. In vitro pull‐down assay presented a clear interaction between polyubiquitin protein and EDIII. To further confirm this interaction, a dot blot assay was employed, and polyubiquitin protein was found to interact with DENV particles. Our results enable us to suggest that polyubiquitin plays an important role in DENV infection within mosquitoes.
RNA sequencing of peripheral blood in amyotrophic lateral sclerosis reveals distinct molecular subtypes: Considerations for biomarker discovery
Authors: Grima Natalie , Liu Sidong , Southwood Dean , Henden Lyndal , Smith Andrew , Lee Albert , Rowe Dominic B. , D'Silva Susan , Blair Ian P. , Williams Kelly L. 
Published: October 2023

AbstractAimAmyotrophic lateral sclerosis (ALS) is a heterogeneous neurodegenerative disease with limited therapeutic options. A key factor limiting the development of effective therapeutics is the lack of disease biomarkers. We sought to assess whether biomarkers for diagnosis, prognosis or cohort stratification could be identified by RNA sequencing (RNA‐seq) of ALS patient peripheral blood.MethodsWhole blood RNA‐seq data were generated for 96 Australian sporadic ALS (sALS) cases and 48 healthy controls (NCBI GEO accession GSE234297). Differences in sALS–control gene expression, transcript usage and predicted leukocyte proportions were assessed, with pathway analysis used to predict the activity state of biological processes. Weighted Gene Co‐expression Network Analysis (WGCNA) and machine learning algorithms were applied to search for diagnostic and prognostic gene expression patterns. Unsupervised clustering analysis was employed to determine whether sALS patient subgroups could be detected.ResultsTwo hundred and forty‐five differentially expressed genes were identified in sALS patients relative to controls, with enrichment of immune, metabolic and stress‐related pathways. sALS patients also demonstrated switches in transcript usage across a small set of genes. We established a classification model that distinguished sALS patients from controls with an accuracy of 78% (sensitivity: 79%, specificity: 75%) using the expression of 20 genes. Clustering analysis identified four patient subgroups with gene expression signatures and immune cell proportions reflective of distinct peripheral effects.ConclusionsOur findings suggest that peripheral blood RNA‐seq can identify diagnostic biomarkers and distinguish molecular subtypes of sALS patients however, its prognostic value requires further investigation.
An examination of the growth of Airbnb in New Zealand and its impact on the private rental housing market: 2016–2021
Authors: Fyfe Caroline , Telfar Barnard Lucy , Howden‐Chapman Philippa , Bennett Julie 
Published: October 2023

AbstractAirbnb is an online platform connecting private hosts with visitors looking for short‐term accommodation. The aim of this paper is to examine the impact of Airbnb on long‐term rental housing availability and cost in Aotearoa New Zealand before and during the COVID‐19 pandemic. Regional growth patterns in Airbnb properties were measured and their impact on the private rental market investigated. An increasing proportion of new rental housing was offered through Airbnb over the study period, particularly in popular tourist locations. Increasing occupancy rates pre‐pandemic suggest that there remains room for growth, putting further pressure on the private rental housing stock.
Virtual reality and noise canceling headphone distraction during pediatric dermatologic procedures
Authors: Jaquez Sasha D. , Haller Courtney N. , England Maryann E. , Bruinsma Raquel L. , Arbet Gregory , Croce Emily A. , Ruth Jennifer , Levy Moise L. , Diaz Lucia Z. 
Published: October 2023

AbstractPain and anxiety related to medical procedures have long been recognized as a significant healthcare concern. If a patient's procedural pain and anxiety are not addressed, long‐term physical and psychological sequelae including increased perceived pain, anxiety, disruptive behavior, trauma reactions, or refusal of future procedures can occur. The objective of our study was to assess the utility of a virtual reality (VR) headset or noise‐canceling headphones (HP) compared to treatment as usual (TAU) in reducing pain and anxiety during pediatric dermatology procedures. Results indicated a significant difference between pre‐ versus post‐procedure anxiety in the VR and HP groups but not the TAU group suggesting non‐pharmacologic technology‐based interventions such as VR and headphones may reduce patients' anxiety during pediatric dermatology procedures.
Self‐management skills in a UK sample of young people with chronic liver disease
Authors: Day Jemma , Hames Anna , Earl Megan , Simpson Anna , Joshi Deepak , Pissas Eleanna , Samyn Marianne 
Published: October 2023

AbstractBackgroundWe aimed to assess self‐management skills and adherence behaviors in young people post‐liver transplant and compare these with those of young people with autoimmune liver disease and other forms of chronic liver disease.MethodAs part of our specialist multidisciplinary clinic, n = 156 young people (aged 16–25 years) completed the Liver Self‐Management Questionnaire (an adaptation of the Developmentally Based Skills Checklist for adolescents post‐liver transplant and modified for us across liver disease type and within the United Kingdom). Those taking medication (n = 128) also completed a service‐designed questionnaire regarding adherence. The statistical significance of group differences was assessed with non‐parametric analyses.ResultsYoung people post‐liver transplant were less likely to report managing their condition independently than those with autoimmune liver disease or those with other forms of chronic liver disease. They also reported higher adherence (93%) compared to those with autoimmune liver disease (77%) and those with other forms of chronic liver disease (85%). However, the vast majority of self‐management and adherence behaviors were comparable between young people post‐transplant and those with autoimmune liver disease/other forms of chronic liver disease.ConclusionOur data are in line with existing data from US samples and also extend these findings to include those with other forms of chronic liver disease. These data highlight the importance of individualized care for young adults, regardless of condition type or healthcare setting, and of clinicians managing their expectations regarding what is considered appropriate condition management in early adulthood.
Street‐level discretion, personal motives, and social embeddedness within public service ecosystems
Authors: Knox Stephen , Arshed Norin 
Published: October 2023

AbstractDrawing on the sense of community responsibility concept, we explore the enterprise policy ecosystem in an extensive qualitative study of Scotland. We present a processual model which explains how policies are shaped in an on‐going dynamic through street‐level managers' individual agency. Our findings reveal that driving the process is an interplay between personal motives (compassion, relational strength, esteem, coherence) with a social frame of reference (policy group, locality, public organization) which is based on embeddedness within specific policy contexts. This interplay guides how managers translate policy as either an opportunity or a threat which then directs how they enact their discretion to adapt, advocate change, or resist implementation. This process offers an explanation as to how situated value is created for specific policy areas within public service ecosystems. The implications are discussed in relation to the existing literature on policy implementation.
Integrated Control Design for a Partially Turboelectric Aircraft Propulsion System
Authors: Simon Donald L. , Bianco Santino J. , Horning Marcus A. 
Published: October 2023

Abstract
Electrified Aircraft Propulsion (EAP) holds great potential for reducing aviation emissions and fuel burn. A variety of EAP architectures have been proposed including partially turbo-electric configurations that combine turbofan engines with motor-driven propulsors. Such architectures exhibit coupling between subsystems and thus require an integrated control solution. To address this need, this paper presents an integrated control design strategy for a commercial single-aisle partially turbo-electric aircraft concept consisting of two wing-mounted turbofan engines and an electric motor driven tailfan propulsor. Within this architecture the turbofans serve the dual purpose of generating thrust and supplying mechanical offtake power used to generate electricity for the tailfan motor. The propulsion control system is tasked with coordinating turbofan and tailfan operation under both steady-state and transient scenarios. The paper introduces a linear state-space representation of the architecture reflecting the coupling between the turbofan and tailfan subsystems along with loop transfer functions reflecting open- and closed-loop system dynamics. Also discussed is an applied strategy for scheduling the tailfan setpoint command based on the average sensed fan speed of the two turbofans. This approach ensures synchronized operation of the turbofan and tailfan subsystems while also allowing the turbofan fuel control design to be simplified. Performance of the integrated control design is assessed through a real-time hardware-in-the-loop test conducted at the NASA Electric Aircraft Testbed. During this test a scaled version of the electrical system and turbomachinery shaft dynamics were implemented in electrical machine hardware and evaluated under closed-loop control. Results from this facility test are presented to illustrate the efficacy of the applied integrated control design approach under steady-state and transient scenarios including a full-flight mission profile.
A Numerical Test Rig for Turbomachinery Flows Based on Large Eddy Simulations With a High-Order Discontinuous Galerkin Scheme—Part I: Sliding Interfaces and Unsteady Row Interactions
Authors: Bergmann Michael , Morsbach Christian , Klose Bjoern F. , Ashcroft Graham , Kügeler Edmund 
Published: October 2023

Abstract
In this first paper of a three-part series, we present the extension and validation of the high-order discontinuous Galerkin scheme in DLR’s CFD-solver trace for scale-resolving simulations of unsteady row interactions. The translational movement of rows in linear cascade experiments is represented in the numerical model by solving the equations in the relative frame of reference. To couple rows in different frames of reference, a sliding interface approach based on the mortar technique for non-conforming meshes has been developed. The verification of the approach is exemplified by three canonical test cases. First, the experimental order of convergence is verified for the isentropic vortex convection. Subsequently, the suitability of the sliding interface approach for scale-resolving simulations is tested on the Taylor–Green vortex flow and a turbulent cylinder flow. Finally, the LES solver is applied to the T106D cascade with upstream moving bars at an exit Reynolds number of 200,000 and exit Mach number of 0.4. The flow physics with and without bars is discussed in terms of the instantaneous flow field, and time- and phase-averaged quantities. The comparison with experimental data shows overall a good agreement, especially for the total pressure losses in the wake, but also reveals uncertainties related to the reproduction of an experiment in the numerical model.
Enterovirus D68 capsid formation and stability requires acidic compartments
Authors: Galitska Ganna , Jassey Alagie , Wagner Michael A. , Pollack Noah , Miller Katelyn , Jackson William T. 
Published: October 2023

ABSTRACT
Enterovirus D68 (EV-D68), a picornavirus traditionally associated with respiratory infections, has recently been linked to a polio-like paralytic condition known as acute flaccid myelitis. EV-D68 is understudied, and much of the field’s understanding of this virus is based on studies of poliovirus. For poliovirus, we previously showed that low pH promotes virus capsid maturation, but here we show that, for EV-D68, inhibition of compartment acidification during a specific window of infection causes a defect in capsid formation and maintenance. These phenotypes are accompanied by radical changes in the infected cell, with viral replication organelles clustering in a tight juxtanuclear grouping. Organelle acidification is critical during a narrow window from 3 to 4 hpi, which we have termed the “transition point,” separating translation and peak RNA replication from capsid formation, maturation, and egress. Our findings highlight that acidification is crucial only when vesicles convert from RNA factories to virion crucibles.

IMPORTANCE
The respiratory picornavirus enterovirus D68 is a causative agent of acute flaccid myelitis, a childhood paralysis disease identified in the last decade. Poliovirus, another picornavirus associated with paralytic disease, is a fecal-oral virus that survives acidic environments when passing from host to host. Here, we follow up on our previous work showing a requirement for acidic intracellular compartments for maturation cleavage of poliovirus particles. Enterovirus D68 requires acidic vesicles for an earlier step, assembly, and maintenance of viral particles themselves. These data have strong implications for the use of acidification blocking treatments to combat enterovirus diseases.
Cytochrome
c
-peroxidase modulates ROS homeostasis to regulate the sexual mating of
Sporisorium scitamineum
Authors: Cai Enping , Jia Huan , Feng Ruqing , Zheng Wenqiang , Li Lei , Zhang Li , Jiang Zide , Chang Changqing 
Published: October 2023

ABSTRACT

Sugarcane smut, caused by the basidiomycetous fungus
Sporisorium scitamineum,
is a global fungal disease resulting in substantial economic losses. Our previous research has highlighted the significant role of reactive oxide species (ROS) in the sexual reproduction of
S. scitamineum
, yet the underlying mechanisms remain unclear. In this study, we identified the cytochrome
c
-peroxidase encoding gene, Ss
CCP1
, as crucial for oxidative stress resistance. We further found that absence of SsCcp1 leads to defects in mating/filamentation and disrupts intracellular ROS homeostasis. However, restoration of mating/filamentation of
SsCCP1
deletion mutants was partially achieved with exogenous antioxidants (vitamin C or vitamin E). Notably, transcription of the pheromone response factor
SsPRF1
, pivotal for mating/filamentation of
S. scitamineum
, is significantly reduced in the Ss
CCP1
deletion mutants or under exogenous hydrogen peroxide (H
2
O
2
) conditions. Nevertheless, the addition of exogenous vitamin C for 60 min significantly up-regulated the
SsPRF1
gene in the Ss
CCP1
deletion mutants. Moreover, the constitutive expression of
SsPRF1
essentially reinstated the mating/filamentation of Ss
CCP1
deletion mutants, suggesting that SsCcp1 mediates the transcriptional activity of
SsPRF1
by maintaining ROS homeostasis to regulate the mating/filamentation of
S. scitamineum
. Further investigations revealed that SsCcp1 is necessary for ROS detoxification and full pathogenicity
in planta
. Overall, this study not only unveils the importance of SsCcp1 in
S. scitamineum
mating/filamentation, ROS detoxification, and virulence but also provides insights into the role of SsCcp1-regulated ROS homeostasis in sexual reproduction of
S. scitamineum
.

IMPORTANCE

Reactive oxygen species play an important role in pathogen-plant interactions. In fungi, cytochrome
c
-peroxidase maintains intracellular ROS homeostasis by utilizing H
2
O
2
as an electron acceptor to oxidize ferrocytochrome
c
, thereby contributing to disease pathogenesis. In this study, our investigation reveals that the cytochrome
c
-peroxidase encoding gene,
SsCCP1
, not only plays a key role in resisting H
2
O
2
toxicity but is also essential for the mating/filamentation and pathogenicity of
S. scitamineum
. We further uncover that SsCcp1 mediates the expression of SsPrf1 by maintaining intracellular ROS homeostasis to regulate
S. scitamineum
mating/filamentation. Our findings provide novel insights into how cytochrome
c
-peroxidase regulates sexual reproduction in phytopathogenic fungi, presenting a theoretical foundation for designing new disease control strategies.