bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood-pool MRI assessment of myocardial microvascular reactivity
Authors: Loai Sadi , Qiang Beiping , Laflamme Michael A. , Cheng Hai-Ling Margaret 
Published: November 2023

PurposeThe ability to non-invasively image myocardial microvascular dilation and constriction is essential to assessing intact function and dysfunction. Yet, conventional measurements based on blood oxygenation are not specific to changes in blood volume. The purpose of this study was to extend to the heart a blood-pool MRI approach for assessing vasomodulation in the presence of blood gas changes and investigate if sex-related differences exist.MethodsAnimals [five male and five female healthy Sprague Dawley rats (200–500 g)] were intubated, ventilated, and cycled through room air (normoxia) and hypercapnia (10% CO2) in 10-minute cycles after i.v. injection of blood-pool agent Ablavar (0.3 mmol/kg). Pre-contrast T1 maps and T1-weighted 3D CINE were acquired on a 3 Tesla preclinical MRI scanner, followed by repeated 3D CINE every 5 min until the end of the gas regime. Invasive laser Doppler flowmetry of myocardial perfusion was performed to corroborate MRI results.ResultsMyocardial microvascular dilation to hypercapnia and constriction to normoxia were readily visualized on T1 maps. Over 10 min of hypercapnia, female myocardial T1 reduced by 20% (vasodilation), while no significant change was observed in the male myocardium. After return to normoxia, myocardial T1 increased (vasoconstriction) in both sexes (18% in females and 16% in males). Laser Doppler perfusion measurements confirmed vasomodulatory responses observed on MRI.ConclusionBlood-pool MRI is sensitive and specific to vasomodulation in the myocardial microcirculation. Sex-related differences exist in the healthy myocardium in response to mild hypercapnic stimuli.
Leydig cell metabolic disorder act as a new mechanism affecting for focal spermatogenesis in Klinefelter syndrome patients: a real world cross-sectional study base on the age
Authors: Liu Huang , Zhang Zhenhui , Gao Yong , Lin Hai , Zhu Zhiyong , Zheng Houbin , Ye Wenjing , Luo Zefang , Qing Zhaohui , Xiao Xiaolan , Hu Lei , Zhou Yu , Zhang Xinzong 
Published: November 2023

BackgroundKlinefelter’s syndrome (KS) was once considered infertile due to congenital chromosomal abnormalities, but the presence of focal spermatozoa changed this. The key to predict and promote spermatogenesis is to find targets that regulate focal spermatogenesis.ObjectiveTo explore the trend of fertility changes in KS patients at different ages and identify potential therapeutic targets.MethodsBibliometric analysis was used to collect clinical research data on KS from the Web of Science Core Collection (WoSCC) from 1992 to 2022. A cross-sectional study was conducted on 75 KS patients who underwent microscopic testicular sperm extraction (mTESE) from 2017 to 2022 in the real world. The reproductive hormones, testicular histopathology, androgen receptors, insulin-like factor 3 (INSL3) receptors and sperm recovery rate (SRR) were analyzed.ResultsMale infertility, dysplasia, Sertoli cells, Leydig cells, testosterone and spermatogenesis were the research focuses related to KS. Luteinizing hormone (LH), testosterone, and INSL3 were evaluation indicators of Leydig cell function that fluctuate with age. Testosterone and LH peaked at ages 13-19 and 30-45, while INSL3 only peaked at ages 13-19. 27 patients (27/75) recovered sperm through mTESE and experienced SRR peaks at the ages of 20, 28, 34, and 37. The SRR of fibrosis patients was 46.15%, fatty degeneration was 7.14%, and melanosis was 40.00%. The INSL3 and androgen receptors were highly expressed and roughly balanced in focal spermatogenesis.ConclusionAbnormal metabolism of Leydig cells led to imbalanced expression of INSL3 and androgen receptors, which might be a potential target for spermatogenesis in KS.
Inspiring citizen science innovation for sustainable development goal 6 in water quality monitoring in China
Authors: Wu Yaqian , Washbourne Carla , Haklay Muki 
Published: November 2023

Introduction: The global challenge of sustainable development is encapsulated in the 17 United Nations Sustainable Development Goals (SDGs), to which China is committed. As outlined in the UNESCO World Water Assessment Program (WWAP) report, water fundamentally impacts on sustainable development, making the achievement of SDG 6 (water and sanitation) crucial. China, as a leading manufacturing hub with extensive agriculture, grapples with challenges in monitoring SDG 6 indicators, especially concerning water quality.Methods: In light of these challenges, this study explores the utilisation of non-traditional data sources, specifically citizen science (CS), to address aspects of SDG 6 monitoring pertaining to water quality monitoring in China. Specifically, our study employs a combination of research techniques–including a literature review, semi-structured interviews, and participant observation–to critically evaluate 13 existing CS projects focused on water quality monitoring. A modified CS assessment framework has been utilised, offering a comprehensive, structured approach to evaluation.Results: In total, this analysis identified four key findings: 1) CS projects related to water monitoring in China predominantly focus on freshwater and drinking water; 2) Greater attention and support are needed for freshwater monitoring initiatives to align with China’s “ecological civilisation” policy; 3) Replacing “citizen science” with “public participation” may promote broader acceptance of these initiatives; 4) Training requires enhancement, particularly for participants from third- and fourth-tier cities as well as remote rural areas.Discussion: This research delineates feasible strategies for the future expansion and integration of CS projects throughout the country. It advocates for a more participatory data-driven approach to sustainable water management in China, given the highlighted challenges and the potential solutions unearthed through the use of citizen science and public participation.
Use of RT-PCR in conjunction with a respiratory pathogen assay to concurrently determine the prevalence of bacteria and SARS-CoV-2 from the nasopharynx of outpatients
Authors: Shurko James F. , Page Robert B. , Mares Chris A. , Nguyen Vivian , Lopez Kristina , Vanee Niti , Mishra Pramod K. 
Published: November 2023

IntroductionCOVID-19 has emerged as a highly contagious and debilitating disease caused by the SARS-CoV-2 virus and has claimed the lives of over 7.7 million people worldwide. Bacterial co-infections are one of many co-morbidities that have been suggested to impact the outcome of COVID-19 in patients. The goals of this study are to elucidate the presence of bacteria in the nasopharynx of SARS-CoV-2 positive and negative patients and to describe demographic categories that may be associated with the detection of these organisms during one of the initial waves of the COVID-19 pandemic.MethodsTo this end, we investigated SARS-CoV-2 and bacterial co-detection from outpatient RT-PCR testing in Texas.ResultsThe results indicate that Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Moraxella catarrhalis, and Haemophilus influenzae were the most frequently detected bacteria in both SARS-CoV-2 positive and SARS-CoV-2 negative patients and that these bacteria were present in these two patient populations at similar proportions. We also detected Staphylococcus aureus in a significantly larger proportion of males relative to females and people under 65 years of age relative to those 65 and over. Finally, we observed that SARS-CoV-2 was more commonly detected in Hispanics compared to non-Hispanics; however, low disclosure rates make volunteer bias a concern when interpreting the effects of demographic variables.DiscussionThis study describes the bacteria present in the nasopharynx of SARS-CoV-2 positive and negative patients, highlights associations between patient demographics and SARS-CoV-2 as well as bacterial co-detection. In addition, this study highlights RT-PCR based molecular testing as a tool to detect bacteria simultaneously when SARS-CoV-2 tests are performed.
Setting sodium targets for pre-packaged foods in China — an exploratory study
Authors: Zhang Puhong , Zhang Jiguo , Rosewarne Emalie , Li Yuan , Dong Le , He Feng J. , Brown Mhairi , Pettigrew Simone , Yamamoto Rain , Nishida Chizuru , Liu Aidong , Yang Xiaoguang , Zhang Bing , Ding Gangqiang , Wang Huijun 
Published: November 2023

IntroductionSetting sodium targets for pre-packaged food has been a priority strategy for reducing population sodium intake. This study aims to explore the attitudes and considerations of researchers and key stakeholders toward implementing such policy in China.MethodsAn exploratory study comprising a survey and a focus group discussion was conducted among 27 purposively selected participants including 12 researchers, 5 consumers, 4 administrators, 3 industry association representatives and 3 food producers. The survey/discussion covered the key questions considered when developing/promoting sodium targets. Free-text responses were manually classified and summarized using thematic analysis.ResultsTwo-thirds of the participants supported target-setting policy. Researchers and administrators were most supportive, and food producers and associations were least supportive. Adapted WHO food categorization framework was well accepted to underpin target-setting to ensure international comparability and applicability for Chinese products. Maximum values were the most agreed target type. The WHO benchmarks were thought to be too ambitious to be feasible given the current food supply in China but can be regarded as long-term goals. Initially, a reduction of sodium content by 20% was mostly accepted to guide the development of maximum targets. Other recommendations included implementing a comprehensive strategy, strengthening research, engaging social resources, establishing a systematic monitoring/incentive system, maintaining a fair competitive environment, and developing a supportive information system. Target-setting policy was acceptable by most stakeholders and should be implemented alongside strategies to reduce discretionary salt use.DiscussionOur findings provide detailed guidance for the Chinese government when developing a target-setting strategy. The methods and results of this study also provide meaningful references for other countries to set sodium targets for pre-packaged foods and implement other salt reduction strategies simultaneously.
Gluten restriction in irritable bowel syndrome, yes or no?: a GRADE-assessed systematic review and meta-analysis
Authors: Arabpour Erfan , Alijanzadeh Dorsa , Sadeghi Amir , Khoshdel Sina , Hekmatdoost Azita , Kord-Varkaneh Hamed , Abdehagh Mohammad 
Published: November 2023

BackgroundMore than half of patients with irritable bowel syndrome (IBS) report aggravating their symptoms with certain foods. Currently, Low fermentable oligo-, di-, and monosaccharides and polyols diet (LFD) is the most accepted dietary intervention for IBS. Recent randomized controlled trials (RCTs) have been suggested that gluten restriction may reduce the symptoms of patients with IBS. However, the results from these studies are conflicting. This study filled this knowledge gap by evaluating the impact of the gluten-free diet (GFD) on IBS symptoms.MethodsA systematic search was carried out in Pubmed/Medline, Cochrane CENTRAL, Scopus, and Web of Science up to April 2023. A random-effect model was applied to estimate the standardized mean difference (SMD) and 95% confidence interval (95% CI) for each outcome.ResultsA total of nine controlled trials were included in the meta-analysis. In contrast to gluten-containing diet, GFD was unable to reduce overall symptoms (SMD − 0.31; 95% CI −0.92, 0.31), bloating (SMD −0.37; 95% CI −1.03, 0.30), and quality of life (SMD −0.12, 95% CI −0.64, 0.39); but had a slight trend to reduce abdominal pain (SMD –0.68; 95% CI −1.36, −0.00). Also, LFD significantly reduced the IBS-Severity score system (SMD 0.66, 95% CI 0.31, 1.01) and improved quality of life (SMD −0.36, 95% CI −0.70, −0.01), compared to GFD.ConclusionA GFD is not robust enough to be routinely recommended for IBS patients, and its efficacy is significantly lower than that of an LFD. Only a certain subgroup of IBS patients may benefit from GFD; further studies are needed to target this subgroup.
CircDDX17 inhibits invasive progression of pituitary adenomas by sponging miR-1279 and regulating CADM2 expression
Authors: Yue Xiao , Lan Fengming , Liu Weiping 
Published: November 2023

PurposeIncreasing evidence has revealed that circDDX17 plays significant regulatory roles in tumor progression. In the present study, we investigated the role of circDDX17 in pituitary adenomas (PAs).MethodsReverse transcription–quantitative PCR was performed to detect the expression of Circular RNA DDX17 (circDDX17), microRNA-1279 (miR-1279), and cell adhesion molecule 2 (CADM2) in PA tissues. Cell abilities of migration and invasion were examined by wound healing and transwell assays. Dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays were applied to confirm the associations among circDDX17, miR-1279, and CADM2. Xenograft tumor experiments were performed to investigate the roles of circDDX17 in vivo.ResultsIn the present study, we found that circDDX17 was downregulated in PA tissues correlated with invasion, tumor size, and progression-free survival of patients with PA. Enforced expression of circDDX17 significantly inhibited migration and invasion through miR-1279. Notably, CADM2 was verified as the direct binding target of miR-1279, and silencing the expression of CADM2 reverses the tumor suppressing effects induced by circDDX17 overexpression. We demonstrated that circDDX17 upregulated the expression of CADM2 by sponging miR-1279, which suppressed the invasive biological behaviors of PA.ConclusionCircDDX17 may serve as a tumor suppressor and potential promising biomarker and effectively therapeutic target for the management of PA.
A prognostic model for tumor recurrence and progression after meningioma surgery: preselection for further molecular work-up
Authors: Padevit Luis , Vasella Flavio , Friedman Jason , Mutschler Valentino , Jenkins Freya , Held Ulrike , Rushing Elisabeth Jane , Wirsching Hans-Georg , Weller Michael , Regli Luca , Neidert Marian Christoph 
Published: November 2023

PurposeThe selection of patients for further therapy after meningioma surgery remains a challenge. Progress has been made in this setting in selecting patients that are more likely to have an aggressive disease course by using molecular tests such as gene panel sequencing and DNA methylation profiling. The aim of this study was to create a preselection tool warranting further molecular work-up.MethodsAll patients undergoing surgery for resection or biopsy of a cranial meningioma from January 2013 until December 2018 at the University Hospital Zurich with available tumor histology were included. Various prospectively collected clinical, radiological, histological and immunohistochemical variables were analyzed and used to train a logistic regression model to predict tumor recurrence or progression. Regression coefficients were used to generate a scoring system grading every patient into low, intermediate, and high-risk group for tumor progression or recurrence.ResultsOut of a total of 13 variables preselected for this study, previous meningioma surgery, Simpson grade, progesterone receptor staining as well as presence of necrosis and patternless growth on histopathological analysis of 378 patients were included into the final model. Discrimination showed an AUC of 0.81 (95% CI 0.73 – 0.88), the model was well-calibrated. Recurrence-free survival was significantly decreased in patients in intermediate and high-risk score groups (p-value &lt; 0.001).ConclusionThe proposed prediction model showed good discrimination and calibration. This prediction model is based on easily obtainable information and can be used as an adjunct for patient selection for further molecular work-up in a tertiary hospital setting.
Test–retest reliability of four flatwater performance-related tests in canoe slalom athletes
Authors: Vajda Matej , Krupa Felix , Busta Jan , Pratt Jaylene 
Published: November 2023

Purpose: This study aims to evaluate the test–retest reliability of four flatwater performance-related tests in canoe slalom athletes. Methods: Twenty-two Slovak national team members of junior and U23 age group racing in a category K1 men (K1M), K1 women (K1W) or C1 men (C1M) volunteered to take part in this study. During both test and retest testing sessions athletes performed 4 flatwater tests: SPS—Sprints with a turn to the preferred side (2 × 15 m shuttle sprints), SNPS—Sprints with a turn to the non-preferred side (2 × 15 m shuttle sprints), SBS—Sprints with turns to both sides (2 × 15 m shuttle sprints) as well as 12 × 15 AOT—12 × 15 m all out shuttle test (12 × 15 m shuttle sprints). Each athlete completed two sessions separated by a minimum of days and a maximum of 5 days. Results: The results have shown the excellent test-retest reliability of all four flatwater tests (ICC—SPS: 0.98; SPNPS 0.97; SBS: 0.98 and 12 × 15 m AOT: 0.96). Additionally, results have shown SEM (SPS: 0.14; SPNPS 0.18; SBS: 0.13 and 12 × 15 m AOT: 1.05) and SWC (SPS: 0.21; SPNPS 0.26; SBS: 0.19 and 12 × 15 m AOT: 1.58). Conclusion: Based on our results we suggest that coaches use these valid and reliable tests to assess changes in their athletes’ performance-related physical fitness over time, to verify the effectiveness of training programs focused on improvement in specific physical fitness of athletes as well as to identify asymmetries between the preferred and non-preferred side in canoe slalom athletes.
Mouse skeletal muscle adaptations to different durations of treadmill exercise after the cessation of FOLFOX chemotherapy
Authors: Halle Jessica L. , Counts Brittany R. , Zhang Quan , James Kylie M. , Puppa Melissa J. , Alway Stephen E. , Carson James A. 
Published: November 2023

FOLFOX (5-fluorouracil, leucovorin, oxaliplatin) chemotherapy is a treatment for colorectal cancer that can induce persistent fatigue and metabolic dysfunction. Regular exercise after chemotherapy cessation is widely recommended for cancer patients and has been shown to improve fatigue resistance in mice. However, gaps remain in understanding whether the early systemic and skeletal muscle adaptations to regular exercise are altered by prior FOLFOX chemotherapy treatment. Furthermore, the effects of exercise duration on early metabolic and skeletal muscle transcriptional adaptations are not fully established.Purpose: Investigate the effects of prior FOLFOX chemotherapy treatment on the early adaptations to repeated short- or long-duration treadmill exercise, including the fasting regulation of circulating metabolic regulators, skeletal muscle COXIV activity and myokine/exerkine gene expression in male mice.Methods: Male C57BL6/J mice completed 4 cycles of FOLFOX or PBS and were allowed to recover for 4-weeks. Subsets of mice performed 14 sessions (6 d/wk, 18 m/min, 5% grade) of short- (10 min/d) or long-duration (55 min/d) treadmill exercise. Blood plasma and muscle tissues were collected 48–72 h after the last exercise bout for biochemical analyses.Results: Long-duration exercise increased fasting plasma osteocalcin, LIF, and IL-6 in healthy PBS mice, and these changes were ablated by prior FOLFOX treatment. Slow-oxidative soleus muscle COXIV activity increased in response to long-duration exercise in PBS mice, which was blocked by prior FOLFOX treatment. Fast-glycolytic plantaris muscle COXIV activity increased with short-duration exercise independent of FOLFOX administration. There was a main effect for long-duration exercise to increase fasting muscle IL-6 and COXIV mRNA expression independent of FOLFOX. FOLFOX administration reduced muscle IL-6, LIF, and BDNF mRNA expression irrespective of long-duration exercise. Interestingly, short-duration exercise suppressed the FOLXOX induction of muscle myostatin mRNA expression.Conclusion: FOLFOX attenuated early exercise adaptations related to fasting circulating osteocalcin, LIF, and IL-6. However, prior FOLFOX treatment did not alter the exercise adaptations of plantaris muscle COXIV activity and plasma adiponectin. An improved understanding of mechanisms underlying exercise adaptations after chemotherapy will provide the basis for successfully treating fatigue and metabolic dysfunction in cancer survivors.
Effects of repetitive transcranial magnetic stimulation on episodic memory in patients with subjective cognitive decline: study protocol for a randomized clinical trial
Authors: Zhang Tianjiao , Huang Sisi , Lu Qian , Song Jie , Teng Jing , Wang Tong , Shen Ying 
Published: November 2023

IntroductionEarly decline of episodic memory is detectable in subjective cognitive decline (SCD). The left dorsolateral prefrontal cortex (DLPFC) is associated with encoding episodic memories. Repetitive transcranial magnetic stimulation (rTMS) is a novel and viable tool to improve cognitive function in Alzheimer’s disease (AD) and mild cognitive impairment, but the treatment effect in SCD has not been studied. We aim to investigate the efficacy of rTMS on episodic memory in individuals with SCD, and to explore the potential mechanisms of neural plasticity.MethodsIn our randomized, sham-controlled trial, patients (n = 60) with SCD will receive 20 sessions (5 consecutive days per week for 4 weeks) of real rTMS (n = 30) or sham rTMS (n = 30) over the left DLPFC. The primary outcome is the Auditory Verbal Learning Test-Huashan version (AVLT-H). Other neuropsychological examinations and the long-term potentiation (LTP)-like cortical plasticity evaluation serve as the secondary outcomes. These outcomes will be assessed before and at the end of the intervention.DiscussionIf the episodic memory of SCD improve after the intervention, the study will confirm that rTMS is a promising intervention for cognitive function improvement on the early stage of dementia. This study will also provide important clinical evidence for early intervention in AD and emphasizes the significance that impaired LTP-like cortical plasticity may be a potential biomarker of AD prognosis by demonstrating the predictive role of LTP on cognitive improvement in SCD.Ethics and disseminationThe study was approved by the Human Research Ethics Committee of the hospital (No. 2023-002-01). The results will be published in peer-review publications.Clinical trial registrationhttps://www.chictr.org.cn/, identifier ChiCTR2300075517.