bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coelomic pouch formation in the starfish Pisaster ochraceus (Echinodermata: Asteroidea)
Authors: Crawford Bruce J. , Chia F. S. 
Published: February 2005

AbstractThe process of coelomic pouch formation in Pisaster ochraceus was studied with light microscopy, transmission and scanning electron microscopy and time‐lapse cinemicrography as well as with the drug cytochalasin B. As in most asteroids, the paired coelomic pouches of Pisaster ochraceus are formed from outpocketing of the archenteron.Arrays of 50 Å microfilaments are found in the presumptive coelomic pouch cells at the apex of the archenteron as well as in the filopodia of the mesenchyme cells. Both cell types undergo active movements throughout the entire process. Treatment of embryos with cytochalasin B (CCB) during coelomic pouch formation results in the loss of cell movements and the regression of the coelomic pouches; this is accompanied by the loss of microfilament arrays in both cell types. Cell movements and microfilament arrays reappear on removal of CCB and coelomic pouch formation resumes.Our evidence suggests that the microfilaments in the presumptive coelomic pouch cells provide the main force for the outpocketing movement. The major role of the microfilament arrays in the filopodia of the mesenchyme cells associated with the coelomic pouches is to determine the definitive shape and location of the pouches.
A histological study of the long‐term response to trauma of squamate integument
Authors: Maderson P. F. A. , Baranowitz S. , Roth S. I. 
Published: February 2005

AbstractScale neogenesis following excision of pieces of skin of various sizes and shapes has been studied in several squamate species. In small wounds, neogenesis does not occur, the wound region contracts, and increased areas of hinge differentiation are seen. In large wounds, the time taken for neogenesis to occur, and the size, shape, distribution, and number of the reformed units, are variable. We confirm previous reports that neogenesis accompanies a renewal phase of the shedding cycle, and suggests that this is because both types of morphogenesis require germinal stability.All types of trauma thus far studied show the initial formation of a hyperplastic wound epithelium with the histological characteristics of a normal α‐layer: such tissues are the basis for physiological barrier functions. The mechanical functions of the skin can be fairly adequately restored even by abnormal neogenic scales. The observed variability in the process of scale neogenesis, and the final form of the individual units, are argued to be the result of mechanical factors impinging on the differentiating tissues.
The relationship of a monoamine fiber system to a somatosensory tectal projection in the salamander Ambystoma tigrinum
Authors: Gruberg Edward R. , Solish Samuel P. 
Published: February 2005

AbstractAfter hemisection of the spinal cord and medulla oblongata, a projection has been traced to the inner half of the tectal white of the tiger salamander, using Fink‐Heimer degeneration staining. By microelectrode recording it was found that the tectal projection forms a topographic somatosensory map of the contralateral half of the body. This map is in register with the overlying retino‐tectal visual projection. Using the Falck‐Hillarp technique, it was found that the somatosensory tectal input is associated with yellow‐fluorescing 5‐hydroxytryptamine fibers.
Reinforced tracheoles in three firefly lanterns: Further reflections on specialized tracheoles
Authors: Ghiradella H. 
Published: February 2005

AbstractThe structures of the lantern tracheoles of three genera of flashing fireflies are compared. All three genera have stiff, reinforced tracheoles which resist folding or collapsing under conditions which flatten more typical tracheoles. This common specialization supports the hypothesis that the tracheoles play a major role in flash control in these fireflies, especially as the morphological basis of the stiffening is different in the three genera. Study of the tracheoles of other tissues reveals that there is great variety in structure and flexibility of these vessels from tissue to tissue and organism to organism, suggesting that tracheolar specialization may be a general phenomenon, with the fine structure of these air tubes being tailored to the particular demands and conditions of the tissues in which they are found.
The fine structure of Gromphadorhina portentosa salivary glands:duct transport system
Authors: Dailey Patrick J. , Crang Richard E. 
Published: February 2005

AbstractThe gross external morphology of the salivary glands of Gromphadorhina portentosa is described from light, scanning, and transmission electron microscopic observations. Various techniques, such as cryofracturing and epoxy‐fracturing followed by plastic removal, were employed. Internally, the transportation system is characterized by a cuticle‐lined lumen bordered by duct cells. The duct collects secretory products, some of which are reabsorbed by duct cells. Products are transported to intercalary ducts and eventually to the hypopharynx and/or salivary reservoirs. Transmission electron micrographs demonstrate distinctive morphological differences between duct cells bordering ductules and those which line expanded regions of the duct. Duct cells which surround ductules have a microvillous‐lined apical border in which the cuticular coat of the lumen may be only partially developed. Duct cells in other regions may retain microvilli, or the apical plasma membrane may invaginate and vesiculate. In some cells the apical region has neither microvilli nor invaginations, but possesses two morphologically different forms of microtubules. Some duct cells are characterized by the presence of lamellar bodies in the nuclear region and/or collagenous material above the basal lamina in the area where the acinar duct becomes confluent with the intercalary duct. The plasma membranes between adjacent duct cells within acini become convoluted, forming loops filled with cytoplasm. These loops, along with contact and septate desmosomes formed between membranes, may serve dual functions: adherent mechanisms between cells and/or transportation of materials between cells.
Histological study of muscular hypoplasia in the crooked neck dwarf mutant (cn/cn) chick embryo
Authors: Wick Robert A. , Allenspach Allan L. 
Published: February 2005

AbstractTibiotarsal segments of 12‐day chick embryos homozygous for the crooked neck dwarf gene (cn/cn) were examined histologically following routine methods of preparation. The myogenic mass fails to divide into separate muscle bundles during the early stages of differentiation. Myoblasts and myotubes are observed, although the proportion favors the mononucleate cell population. Multinucleate myotubes are often wavy in appearance and many contain eosinophilic cytoplasmic inclusions. The entire tissue mass of mutants appears more compacted than in control limbs. Poor organization of muscle appears related to the lack of a suitable connective tissue system. Epimysia, perimysia, and subcutaneous connective tissue fail to develop properly. Tendons are poorly developed or absent. Comparisons between mutant and control embryos show no differences in peripheral innervation. Nerve fascicles penetrate deeply into the developing muscle of both species. The distribution of vascular elements is seemingly normal also. Skeletal muscle of cn/cn embryos is capable of differentiating to the myotube stage, after which it undergoes cellular degeneration without achieving a functional state. Comparisons of this mutant with alleged chemical phenocopies show important differences.
Seasonal ultrastructural variations in pinealocytes of the woodchuck, Marmota monax
Authors: Frink R. , Krupp P. P. , Young R. A. 
Published: February 2005

AbstractThe ultrastructure of the pinealocyte in the woodchuck, Marmota monax, was studied during the four seasons of the year. Fall cells have a fairly uniform cytoplasmic density, organelles consistent with synthetic and/or secretory activity and rather extensive pericapillary and intercellular spaces. Many winter pinealocytes are nearly devoid of ribosomes and granular endoplasmic reticulum but contain lipid droplets associated with mitochondria. Pericapillary and intercellular spaces are minimal. Spring glands have the greatest variation in cytoplasmic density with intercellular and pericapillary spaces similar to that seen in fall glands. Cells containing electron dense cytoplasm have Golgi zone associated, secretory granules, free ribosomes, short sections of granular endoplasmic reticulum and dense bodies. Cells with a more electron lucent cytoplasm are similar to the most frequently observed summer pinealocytes which have numerous Golgi zones but few associated secretory granules. Microtubules are prominent in the cytoplasm of these cells, the plasma membranes are smooth and intercellular and pericapillary spaces are minimal. A yearly rhythm or cyclic activity of the pinealocyte is suggested.
Fine structure and metamorphosis of the wax gland cells in a psyllid insect, Anomoneura mori schwartz (Homoptera)
Authors: Waku Yoshio 
Published: February 2005

AbstractThe fine structure of the wax gland of Anomoneura nymph and its metamorphic change were investigated. In the nymph, this organ encircles the anus, and consists of two kinds of cells, derived from epidermal cells: (1) very tall, slim wax cells, which produce and secrete the wax, and (2) flat interstitial cells found among the wax cells. The whole gland is covered by a wax‐secreting cuticle with a delicate surface sculpture. Each wax cell has a long, wide duct which opens at the cuticle and penetrates the entire cell. Its cytoplasm is rich in mitochondria and smooth endoplasmic reticulum while that of interstitial cells contains rough endoplasmic reticulum. During each nymphal molt, the cluster of primordial wax gland cells — derived from the epidermis — proliferates rapidly and forms the gland of the next instar. The gland of the preceding instar meanwhile degenerates. Interstitial cells play an important role in cuticle formation and shedding at each molt. These cells alone produce and deposit the new cuticle of the next instar; the wax cells, specialized for wax production, cannot produce cuticle. The apical portion of the wax cell is cut off from the main cell body by growth of the surrounding interstitial cells. Thereafter, the wax cells degenerate, resulting in the rapid disappearance of the previous instar's wax gland. Adults lack this gland entirely.
Ultrastructural features of neuromuscular junctions in the stapedius muscle of Gallus gallus
Authors: Counter S. Allen 
Published: February 2005

AbstractThe ultrastructure of neuromuscular junctions in the twitch fibers of the stapedius muscle of Gallus gallus (domesticus) was investigated as part of a series of neurophysiological studies. Among the morphological features observed were elongated end‐plates with numerous large and clear synaptic vesicles mixed with larger dense core vesicles and irregular or aperiodic “active sites” in the presynaptic membrane where synaptic vesicles were focused. The most remarkable features of these junctions were large synaptic clefts (50‐80 nm) and the absence of junctional folds in the sarcolemmal surface. Unlike the large periodic junctional folds seen in the neuromuscular junctions of frogs and in the fast twitch fibers of the mammalian stapedius, the preparations studied only show small aperiodic invaginations (primitive folds) in the postsynaptic membranes. This morphological feature remains essentially constant from newly hatched to adult chickens. While these smooth junctions are consistent with earlier findings of inconspicuous junctional folds in the twitch fibers of the chicken posterior latissimus dorsi they are unlike those seen in the fast twitch fibers of the mammalian stapedius muscle, or other twitch fibers in general. The morphological findings of the present study may also suggest that the simple, unmodified neuromuscular junctions in the stapedius of Gallus may be a useful preparation for studies of synaptic membrane structures that employ the freeze‐fracture technique.
Ultrastructural investigation of spermiogenesis in Peripatopsis capensis (Onychophora)
Authors: Camatini Marina , Franchi Emilia , Saita Abele 
Published: February 2005

AbstractEarly spermatids of the onychophoran Peripatopsis capensis are spherical cells with a centrally located nucleus, numerous mitochondria, Golgi complexes, microtubules and two centrioles. During spermiogenesis, Golgi vesicles migrate to one side of the cell where they form a tight aggregate, which is later shed. The mature spermatozoon has no acrosome. Several mitochondria fuse to form a middle piece containing three large mitochondria. Nucleus and middle‐piece elongate, presumably under the influence of helically twisted microtubules. Outside this set of microtubules a continuous layer of endoplasmic reticulum cisternae is formed which separates the interior portion of the cell from an external cytoplasmic rim, which is later shed. Outside the 9 + 2 complex, the tail presents nine accessory microtubules, and a peripheral layer of microtubules beneath the plasma membrane. The enforcement of the tail structure may be related to the fertilization biology of this animal, which is by “hypodermal” impregnation.
Ultrastructure of the accessory glands of the Mediterranean flour moth
Authors: Riemann John G. , Thorson Barbara J. 
Published: February 2005

AbstractFive regions are recognized in the accessory glands of the Mediterranean flour moth, Anagasta kuehniella (Zeller), on the basis of cellular morphology and aggregates of secretory material in the lumen. Some variation is found in each of the posterior four regions, especially the third one. In the most anterior region (region 1) the epithelium is composed of a single type of cell, while in each of the other regions there are two classes of cells. The cells of region 1 and one class in each of the other four regions are fairly typical exocrine cells with extensive rough endoplasmic reticula. Secretion is primarily via Golgi‐derived vesicles. Apocrine secretion in the form of sloughing off of the apical cytoplasm probably also occurs in all regions but is most prominent in the posterior two regions. One class of cells is very similar in morphology in each of the posterior four regions though their secretory products form characteristic aggregates in the lumen. The second class of cells (foliate cells) occurring in the posterior four segments is most notably characterized by elongate apical projections that extend out into the lumen. The apical projections contain large quantities of glycogen, some microtubules, and, in some cases, many minute mitochondria. The membrane content of the projections is also very high. In the anterior regions, the membranes are mostly fused in pairs and typically form multilayered whorls. Fusion and whorl formation decrease in the posterior regions. The cytoplasm of the foliate cells has a high organelle content including many lysosomes and mitochondria. The latter exhibit considerable polymorphism, with particular forms occurring in the different regions of the glands. The apical projections of the foliate cells are detached during copulation, presumably as the result of nervous stimulation, and become a part of the ejaculate. Replenishment of all secretory material, including the apical projections, occurs after copulation.
Fibrous and adhesive components of the orb webs of Araneus trifolium and Argiope trifasciata
Authors: Kavanagh Edward J. , Tillinghast Edward K. 
Published: February 2005

AbstractThe minimum number of secretion products used by the spiders Araneus trifolium and Argiope trifasciata to construct their orb webs has been established by selective enzyme digestion and histochemical staining, as well as differential isotope localization in these webs. Three fiber types are present in the webs: (a) a major fiber found throughout the web, (b) a minor fiber found only in radial threads, and (c) the core fibers of the sticky spiral thread. Three nonfibrous secretions are found on these fibers. These include a water soluble viscid coating of the sticky spiral and two adhesives which fasten the threads of the web together; one found only at junctions of sticky spiral and radial threads and the other at all other thread connections. The possible glandular sources of these secretions are discussed.
A model to explain the posterior limit of the bite point in reptiles
Authors: Druzinsky Robert E. , Greaves W. S. 
Published: February 2005

AbstractA biomechanical model of the jaw mechanism in some reptiles is presented. Symmetrical muscle activity that produces equal forces on both sides of the head is assumed. The model predicts the position of the most posterior bite point and offers a functional explanation for this prediction. Turtles are used to illustrate the idea.
The functional significance of primate mandibular form
Authors: Hylander William L. 
Published: February 2005

AbstractA stress analysis of the primate mandible suggests that vertically deep jaws in the molar region are usually an adaptation to counter increased sagittal bending stress about the balancing‐side mandibular corpus during unilateral mastication. This increased bending stress about the balancing side is caused by an increase in the amount of balancing‐side muscle force. Furthermore, this increased muscle force will also cause an increase in dorsoventral shear stress along the mandibular symphysis. Since increased symphyseal stress can be countered by symphyseal fusion and as increased bending stress can be countered by a deeper jaw, deep jaws and symphyseal fusion are often part of the same functional pattern. In some primates (e.g., Cercocebus albigena), deep jaws are an adaptation to counter bending in the sagittal plane during powerful incisor biting, rather than during unilateral mastication.The stress analysis of the primate mandible also suggests that jaws which are transversely thick in the molar region are an adaptation to counter increased torsion about the long axis of the working‐side mandibular corpus during unilateral mastication. Increased torsion of the mandibular corpus can be caused by an increase in masticatory muscle force, an increase in the transverse component of the postcanine bite force and/or an increase in premolar use during mastication.Patterns of masticatory muscle force were estimated for galagos and macaques, demonstrating that the ratio of working‐side muscle force to balancing‐side muscle force is approximately 1.5:1 in macaques and 3.5:1 in galagos during unilateral isometric molar biting. These data support the hypothesis that mandibular symphyseal fusion is an adaptative response to maximize unilateral molar bite force by utilizing a greater percentage of balancing‐side muscle force.
Presence and development of a sensory formation in the eyestalk of the crayfish, Astacus leptodactylus (Nordmann, 1842), during its embryonic, larval and adult life
Authors: van Herp François , Bellon‐Humbert Chantal , De Groot Adrianus J. M. 
Published: February 2005

AbstractA sensory papilla is described in the eyestalk of the crayfish Astacus leptodactylus during the last embryonic stages and during larval stages by light microscopy. This region was also investigated with the scanning electron microscopy, which showed sensory hairs in the postmolt adult; they disappear during intermolt and premolt. Simultaneous cyclic changes in hair papillae are observed in the hypodermis. The possibility of a chemoreceptive function is discussed.
Ultrastructure of the parathyroid gland of the japanese lizard in the spring and summer season
Authors: Isono Hideo , Shoumura Shizuko , Ishizaki Noriko , Hayashi Kazuko , Yamahira Tomo 
Published: February 2005

AbstractThe ultrastructure of the parathyroid glands of adult Japanese lizards (Takydromus tachydromoides) in the spring and summer season was examined. The parenchyma of the gland consists of chief cells arranged in cords or solid masses. Many chief cells contain numerous free ribosomes and mitochondria, well‐developed Golgi complexes, a few lysosome‐like bodies, some multivesicular bodies and relatively numerous lipid droplets. The endoplasmic reticulum is mainly smooth‐surfaced. Cisternae of the rough endoplasmic reticulum are distributed randomly in the cytoplasm. Small coated vesicles of 700‐800 Å in diameter are found occasionally in the cytoplasm, especially in the Golgi region. The chief cells contain occasional secretory granules of 150‐300 nm in diameter that are distributed randomly in the cytoplasm and lie close to the plasma membrane. Electron dense material similar to the contents of the secretory granules is observed in the enlarged intercellular space. These findings suggest that the secretory granules may be discharged into the intercellular space by an eruptocrine type of secretion. Coated vesicles (invaginations) connected to the plasma membrane and smooth vesicles arranged in a row near the plasma membrane are observed. It is suggested that such coated vesicles may take up extracellular proteins. The accumulation of microfilaments is sometimes recognized. Morphological evidence of synthetic and secretory activities in the chief cells suggests active parathyroid function in the Japanese lizard during the spring and summer season.
The mouthparts of female Corethrella brakeleyi and C. wirthi (Diptera: Chaoboridae)
Authors: McKeever Sturgis , Pound J. Mathews 
Published: February 2005

AbstractThe mouthparts of female Corethrella brakeleyi and C. wirthi were studied using light and electron microscopy. Mandibles, hypopharynx and labium are highly sclerotized and are modified for obtaining blood meals. All structures were larger in C. brakeleyi than in C. wirthi except mandibular and hypopharyngeal teeth; these were smaller and more numerous in C. brakeleyi. The labium of both species terminates in peg‐like structures which are similar to those reported from several genera of mosquitoes. Sensillae on the second segment of the maxillary palps appear to be identical to those described in both biting and nonbiting male and female blackflies.
The morphology of the sex pheromone gland in Drosophila grimshawi
Authors: Hodosh R. J. , Keough E. M. , Ringo J. M. 
Published: February 2005

AbstractThe morphology of a sex pheromone‐producing gland found in the abdomen of Drosophila grimshawi males was studied by light and electron microscopy. This gland, consisting of two intra‐anal lobes, contains cells that resemble those of other insect pheromone glands. However, in contrast to many other insect pheromone glands that release pheromone through the cuticle, cells of the intra‐anal lobes secrete into a canaliculi‐duct system that empties into the anal region. The liquid secretory product flows along the surface of the intra‐anal lobes and is brushed onto the substrate by fingerlike projections on the lobes' surfaces.