bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome sequences of equine influenza A subtype H3N8 viruses by long read sequencing and functional characterization of the PB1-F2 virulence factor of A/equine/Paris/1/2018
Authors: Kleij Lena , Bruder Elise , Raoux-Barbot Dorothée , Lejal Nathalie , Nevers Quentin , Deloizy Charlotte , Costa Bruno Da , Legrand Loïc , Barrey Eric , Chenal Alexandre , Pronost Stéphane , Delmas Bernard , Dhorne-Pollet Sophie 
Published: October 2023

AbstractEquine influenza virus (EIV) remains a persistent threat to equines, despite the availability of vaccines. Currently, strategies to monitor the virus and prevent any potential vaccine failure revolve around serological assays, RT‒qPCR amplification, and sequencing the viral hemagglutinin (HA) and neuraminidase (NA) genes. These approaches overlook the contribution of other viral proteins in driving virulence. This study assesses the potential of long-read nanopore sequencing for swift and precise sequencing of circulating equine influenza viruses. To this end, two French Florida Clade 1 strains, including the one circulating in winter 2018-2019 exhibiting more pronounced pathogenicity than usual, as well as the two currently used OIE-recommended vaccine strains, were sequenced. Our results demonstrated the reliability of this sequencing method in generating accurate sequences. Sequence analysis of HA revealed a subtle antigenic drift in the French EIV strains, with specific substitutions, such as T163I in A/equine/Paris/1/2018 and the N188T mutation in post-2015 strains; both substitutions were located in antigenic site B. Antigenic site E exhibited modifications in post-2018 strains, with the N63D substitution. Segment 2 sequencing also revealed that the A/equine/Paris/1/2018 strain encodes a longer variant of the PB1-F2 protein when compared to other Florida clade 1 strains (90 amino acids long versus 81 amino acids long). Further biological and biochemistry assays demonstrated that this PB1-F2 variant has enhanced abilities to abolish the mitochondrial membrane potential ΔΨm and permeabilize synthetic membranes. Altogether, our results highlight the interest in rapidly characterizing the complete genome of circulating strains with next-generation sequencing technologies to adapt vaccines and identify specific virulence markers of EIV.
Accelerated drug resistant variant discovery with an enhanced, scalable mutagenic base editor platform
Authors: Dorighi Kristel M. , Zhu Anqi , Fortin Jean-Philippe , Lo Jerry Hung-Hao , Sudhamsu Jawahar , Durinck Steffen , Callow Marinella , Foster Scott A. , Haley Benjamin 
Published: October 2023

ABSTRACTPersonalized cancer therapeutics bring directed treatment options to patients based on the genetic signatures of their tumors. Unfortunately, tumor genomes are remarkably adaptable, and acquired resistance to these drugs through genetic means is an all-too-frequent occurrence. Identifying mutations that promote resistance within drug-treated patient populations can be cost, resource, and time intensive. Accordingly, base editing, enabled by Cas9-deaminase domain fusions, has emerged as a promising approach for rapid, large-scale resistance variant screening in situ. We adapted and optimized a conditional activation-induced cytidine deaminase (AID)-dCas9 system, which demonstrated greater heterogeneity of edits with an expanded footprint compared to the most commonly utilized cytosine base editor, BE4. When combined with a custom sgRNA library, we were able to identify both individual and complex, compound variants in EGFR and BRAF that confer resistance to established EGFR inhibitors. This system and the developed analytical pipeline provide a simple, highly-scalable platform forcisortransdrug-modifying variant discovery and for uncovering unique insights into protein structure-function relationships.
Basigin mediation ofPlasmodium falciparumred blood cell invasion does not require its interaction with monocarboxylate transporter 1
Authors: King Nadine R , Freire Catarina Martins , Touhami Jawida , Sitbon Marc , Toye Ashley M , Satchwell Timothy J 
Published: October 2023

AbstractPlasmodium falciparuminvasion of the red blood cell is reliant upon the essential interaction of PfRh5 with the host receptor protein basigin. Basigin exists as part of one or more multiprotein complexes, most notably through interaction with the monocarboxylate transporter MCT1. However, the potential requirement for basigin association with MCT1 and the wider role of basigin host membrane context and lateral protein associations during merozoite invasion has not been established. Using genetically manipulatedin vitroderived reticulocytes, we demonstrate the ability to uncouple basigin ectodomain presentation from the transmembrane domain-mediated interaction with MCT1. Merozoite invasion of reticulocytes is unaffected by disruption of basigin MCT1 interaction and by removal or replacement of the basigin transmembrane helix. Therefore, presentation of the basigin ectodomain at the red blood cell surface, independent of its native association with MCT1 or other interactions mediated by the transmembrane domain, is sufficient to facilitate merozoite invasion.
GCN5L1-mediated acetylation prevents Rictor degradation in cardiac cells after hypoxic stress
Authors: Bugga Paramesha , Manning Janet R. , Mushala Bellina A.S. , Stoner Michael W. , Sembrat John , Scott Iain 
Published: October 2023

AbstractCardiomyocyte apoptosis and cardiac fibrosis are the leading causes of mortality in patients with ischemic heart disease. As such, these processes represent potential therapeutic targets to treat heart failure resulting from ischemic insult. We previously demonstrated that the mitochondrial acetyltransferase protein GCN5L1 regulates cardiomyocyte cytoprotective signaling in ischemia- reperfusion injuryin vivoand hypoxia-reoxygenation injuryin vitro. The current study investigated the mechanism underlying GCN5L1-mediated regulation of the Akt/mTORC2 cardioprotective signaling pathway. Rictor protein levels in cardiac tissues from human ischemic heart disease patients were significantly decreased relative to non-ischemic controls. Rictor protein levels were similarly decreased in cardiac AC16 cells following hypoxic stress, while mRNA levels remained unchanged. The reduction in Rictor protein levels after hypoxia was enhanced by the knockdown of GCN5L1, and was blocked by GCN5L1 overexpression. These findings correlated with changes in Rictor lysine acetylation, which were mediated by GCN5L1 acetyltransferase activity. Rictor degradation was regulated by proteasomal activity, which was antagonized by increased Rictor acetylation. Finally, we found that GCN5L1 knockdown restricted cytoprotective Akt signaling, in conjunction with decreased mTOR abundance and activity. In summary, these studies suggest that GCN5L1 promotes cardioprotective Akt/mTORC2 signaling by maintaining Rictor protein levels through enhanced lysine acetylation.
The influence of library service quality, library image, place, personal control and trust on loyalty: the mediating role of perceived service value and satisfaction
Authors: Malik Shahab Alam , Fatima Taqdees , Jia Yong , Pannu Hina 
Published: October 2023

PurposeOne of the organization's main goals is to maintain their customers' loyalty, as this can give them a competitive advantage. Therefore, this study is intended to look into the impact of library service quality using LibQUAL + TM dimensions (library service effect, personal control and library as a place), library image and trust on users’ loyalty with the mediating effect of perceived service value and satisfaction.Design/methodology/approachData for hypotheses testing were gathered from Minhaj University Lahore (MUL), a private sector university's staff, administration and students, using a survey questionnaire. About 500 questionnaires were randomly distributed, and 407 were utilized as the final sample for analysis. Structural equation modelling (SEM), using SmartPLS 4 and SPSS 25, were used to analyze the empirical data.FindingsFirst, research reveals that library image, users’ trust, library service effect and personal control significantly influence user satisfaction. Second, the library as a place and personal control are not a significant indicator of perceived service value. Third, perceived service value and satisfaction have a direct positive relationship with users' loyalty. Fourth, trust and library service effect is indirectly related to loyalty via perceived service value. Fifth, satisfaction does not mediate the relationship between library as a place and loyalty.Practical implicationsCustomer satisfaction must be guaranteed by library administration if it hopes to win users’ loyalty. In light of the fact that a variety of factors can affect customer satisfaction, their perception and loyalty, library management should enhance not just the quality of library services along with other factors such as library image and users’ trust.Originality/valueThe study examined the independent impact of library image and users’ trust on satisfaction, perceived service value and users’ loyalty, which, within the framework of the library, has never been addressed in literature.
A systematic review of the trends and patterns of sustainability reporting in universities
Authors: Rosa Marcos Roque da , Boscarioli Clodis , Freitas Zara Kátya Regina de 
Published: October 2023

Purpose
This study aims to identify how literature has addressed sustainability reporting in universities over time and determine traceable patterns and trends.

Design/methodology/approach
A comprehensive systematic review protocol of the Emerald Insight, Web of Science, Science Direct, Scopus, Springer Link and Wiley Online Library databases was performed for the studies published during the past decade. The following steps were involved in this study: search and collection of literature, study selection, quality assessment, data extraction and data analysis. A total of 40 articles met the inclusion criteria and the quality assessment.

Findings
The results demonstrated that the most used tools to assist sustainability reporting in universities are the global reporting initiative framework and the graphical assessment of sustainability in universities. However, the adoption of the integrated reporting framework is encouraged. The level of reporting is not yet high, and social and educational indicators are being disclosed in the following areas: community, services and transfer; curriculum and teaching; operation; and research.

Originality/value
This study provides a recent overview of sustainability reporting worldwide. By discussing the findings, the authors presented the practical implications of starting a sustainability report at the university and the associated difficulties and benefits.
Gamify, engage, build loyalty: exploring the benefits of gameful experience for branded sports apps
Authors: Habachi Salma , Matute Jorge , Palau-Saumell Ramon 
Published: October 2023

Purpose
This study aims to examine the impact of the gameful experience on behavioural outcomes. Drawing from stimulus–organism–response theory, it proposes and tests a new model that investigates the relationship between the gameful experience, brand loyalty and intention to use gamified branded applications in the sports context. In addition, it explores the mediating role of customer–brand engagement (CBE) and the moderating role of self-image congruity (SIC).

Design/methodology/approach
A sample of 436 active users of sport-related branded gamified applications was used to test the model. Data was collected from online sports forums, brands’ Facebook communities and during sporting events.

Findings
Results indicate that the gameful experience positively and directly impacts behavioural intentions but does not directly influence brand loyalty. This relationship becomes partially significant when mediated by CBE. In addition, results show that users with high levels of SIC are more likely to continue using the gamified application, whereas users with low levels are more likely to engage with the brand.

Originality/value
This study expands the gamification literature in the sports sector by revealing the importance of the gameful experience in driving loyalty, behavioural intentions and CBE. It proposes a new model that sheds light on the emotional aspect of the interaction between a user and a gamified system and the importance of exploring the effects of moderators, such as SIC, in these relationships.
Pain and associated functional impairment in the Danish general population: the role of mental well-being
Authors: Santini Ziggi Ivan , Ekholm Ola , Koyanagi Ai , Stewart-Brown Sarah , Nielsen Line , Meilstrup Charlotte , Koushede Vibeke , Thygesen Lau Caspar 
Published: October 2023

Purpose
Prior research on relations between mental health and pain has focused on negative mental health aspects (e.g. depression), while the literature is scarce in terms of positive aspects, such as mental well-being. This study aims to investigate prospective associations of mental well-being at baseline with pain and functional impairment due to pain in the following year.

Design/methodology/approach
Data stem from a Danish nationally representative survey of 5,000 adults (aged 15+) conducted in 2019 and 2020, which was linked to register data. The Short Warwick–Edinburgh Mental Well-being Scale was used to assess mental well-being, both continuously (range 7–35) and categorically (low, moderate, high). Logistic regressions were conducted to assess associations between mental well-being in 2019 and pain and functional impairment due to pain (among those reporting any pain) in 2020.

Findings
In the fully adjusted models, each point increase in mental well-being was inversely associated with pain (OR = 0.97, 95%CI 0.95–0.99) and functional impairment due to pain (OR = 0.96, 95%CI 0.94–0.99). As compared to low mental well-being, moderate mental well-being was associated with an OR of 0.76 (95%CI 0.58–0.99) for pain and an OR of 0.63 (95%CI 0.46–0.87) of functional impairment due to pain, while high mental well-being was associated with an OR of 0.56 (95%CI 0.40–0.77) for pain and an OR of 0.53 (95%CI 0.34–0.79) for functional impairment due to pain.

Originality/value
Higher mental well-being levels may be protective against pain and functional impairment due to pain. Wider mental health promotion may be considered to prevent pain and associated functional impairments in the general population.
Perpetrators' perspective on cyberbullying: a qualitative systematic review with bibliometric analysis
Authors: Bansal Suhans , Garg Naval , Singh Jagvinder 
Published: October 2023

PurposeCyberbullying has become one of the reasons behind the increase in psychological and medical problems. A need to prevent recurrences of cyberbullying incidents and discourage bullies from further bullying the victims has risen. This problem has attracted the attention of all stakeholders across the globe. Various researchers have developed theories and interventions to detect and stop bullying behavior. Previously, researchers focused on helping victims, but as the times have changed, so has the focus of researchers. This study aims to analyze scientific research articles and review papers to understand the development of the knowledge base on the topic.Design/methodology/approachThis study analyzes the performance of literature on cyberbullying perpetration (CBP) using the widely accepted bibliometric analysis techniques: performance analysis and science mapping. The study is based on a dataset extracted from the Web of Science database. Initially, 2,792 articles between 2007 and 2022 were retrieved, which were filtered down to 441. The filter was based on various criteria, but primarily on CBP. VOSViewer and MS Excel were used to analyze the data. In addition, VOSViewer was used to create “bibliometric citations, co-citations, and co-word maps.”FindingsThe findings include publication and citation quantum and trends, the top 20 active countries, the most significant research articles and leading journals in this domain. Major themes or clusters identified were “Cyberbullying and victim behavior,” bullying behavior, adolescents and intervention, “cyberbullying associations,” and “cyberbullying personality associations.”Originality/valueThe study is unique because it analyses research articles based on cyberbullies, whereas past studies explored only the victims' side. Further, the present study used the Web of Science database, whereas most studies use the Scopus database.
The role of headquarters in managing coopetition within MNCs: a tale of two paradoxes
Authors: Schweizer Roger , Lagerström Katarina , Leite Emilene , Pahlberg Cecilia 
Published: October 2023

Purpose
The purpose of this paper is to contribute to the discussion on how multinational company (MNC) headquarters (HQs) can manage the existing coopetition paradox to ensure innovation within the MNC. In contrast to the rather scarce previous research, the authors argue that HQ needs to solve the coopetition paradox under the sway of a parenting paradox. Hence, HQ faces a dual paradox.

Design/methodology/approach
Drawing on the literature on HQ’s role during MNCs’ innovation processes, this conceptual paper revisits the previously suggested HQ measures to enable coopetition among subsidiaries. By applying a sheer ignorance perspective, the authors contribute with a more nuanced understanding of the HQ’s role in innovation activities.

Findings
The article identifies four challenges as the HQ faces a parenting paradox that hinders its ability to solve the coopetition paradox: context specificity of subsidiaries’ innovation work, normative expectations of subsidiary managers, potential opportunistic behavior of HQ manager and HQ underestimation of needed resources. The article suggests that HQ needs to become more informed and preferably even embedded in the local innovation networks of its most important subsidiaries and that coopetition should not be managed solely on an HQ level.

Originality/value
Advocating a sheer ignorance perspective, the article pioneers in discussing the role that HQ plays in managing coopetition among subsidiaries in innovation activities.
Local communities at the heart of tourism development: the Azores tourism strategy 2030
Authors: Cabral Berta 
Published: October 2023

PurposeThis paper aims to present the Azores tourism strategy and outline how local communities are centered at the heart of tourism development.Design/methodology/approachThis paper analyzes the development of tourism in the Azores Archipelago and how it contributes to value creation and retention, as well as job creation. It explores the role of local communities in the tourism experience, and the strategic options on the 2030 horizon.FindingsTourism has become a main driver for economic growth and social prosperity in the Azores, with sustainability acting as a definitive driver of tourism development. However, to fully leverage the potential of tourism to impact local communities and the regional economy, it is of the utmost importance to stimulate the dispersal of inbound tourism across the nine islands. This will help to spread out value and job creation, help in reducing pressure on fragile resources and at the same time, mitigate seasonality and provide more stable income throughout the year.Originality/valueA new approach to tourism development is proposed, where the success of tourism is to be measured by the quality of the tourist experience and the benefits that tourism adds to the Azores, assigning the local community with a central role in decision-making processes related to tourism. To reinforce the focus on the sustainable development of the territory, anchored on its certification as a sustainable tourist destination, the first archipelago in the world with this distinction, a strong focus on Nature (Land and Sea), is adopted as the main priority. Communication with the end consumer is strongly proposed as a way of stimulating the desire to travel, supported by the positioning proposed in the new tourism strategy (PEMTA, 2030) – Azores: Nine islands, the experience of a lifetime.
MAC in India: a rocky road or smooth ride
Authors: Srivastava Shefali , Singh Bindu 
Published: October 2023

Purpose
Known for its motto “All Ages, All Races, All Genders,” MAC cosmetics reflects the brand’s commitment to inclusivity and diversity. It has been at the forefront of promoting a wide range of skin tones, ethnicities and gender identities in the beauty industry. If you have ever wondered about the secret behind the success of this mega-brand in the beauty industry, this case study aims to provide a comprehensive overview of the company’s history from its early days to the present and discusses the factors that might build or ruin a brand. Moreover, players in the industry will need to adapt to changing consumer preferences, focus on sustainability and leverage digital technologies to remain relevant and capture market opportunities. Therefore, would it be wise for MAC cosmetics to take forward their expansion plans in India, and what is the motivation behind the expansion? Alternatively, would the implementation of a proactive strategy for product innovation, combined with a stronger presence, result in a more viable and sustainable proposition?

Design/methodology/approach
This study relies on online resources for data collection. The data was collected from the secondary sources in the month of November and December 2022. Moreover, the information provided in the case study was validated and supplemented via using different websites, including the company’s website and social media profiles.

Findings
The commitment shown and the roles played will have a lasting impact on the society. Additionally, student will be able to learn when and how to seize opportunities, as well as how to manage obstacles that will arise in their entrepreneurial journey. Through this case study, they will be able to learn a variety of business strategies that can be implemented in emerging markets. The conclusion of the study provides the obstacles and hurdles faced by the industry in expanding in the Indian market. The potential solutions were made through providing information for the students, academicians, entrepreneurs and others to compete in the Indian market situation.

Originality/value
This is a real-world case that provides the audience with first-hand experience to comprehend the event and apply their knowledge to form a conclusion and is also effective for decision-making in the dynamic environment of the present day. However, the authors provide a comprehensive overview of the company’s history from its early days to the present and discuss the factors that might build or ruin a brand.
Loss functions in machine learning for seismic random noise attenuation
Authors: Jun Hyunggu , Kim Han‐Joon 
Published: October 2023

AbstractSeismic random noise is one of the main factors that degrade the quality of seismic data. Therefore, seismic random noise attenuation should be performed appropriately through several stages during seismic data processing, and this requires sufficient experience and knowledge because the proper hyperparameters need to be determined based on the features of the noise in the target seismic data. Recently, machine learning–based seismic noise attenuation has been widely studied because it provides suitable results by learning noise features from noisy data, unlike conventional physics‐based approaches. There are many important factors in machine learning, and, here, we focus on the loss functions of machine learning in terms of seismic random noise attenuation. The most widely used loss function is l2, but we train a model with various kinds of single and multiple loss functions and attenuate seismic random noise. We analyse the efficiency of loss functions by comparing the noise‐attenuated results of synthetic and field seismic data qualitatively and quantitatively. Our analysis indicates that the multiple loss function with the l1 norm can be a proper choice for random noise suppression of seismic data.