bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resource availability as a condition for the effectiveness of professional activity of the senior staff of departments and faculties of educational organizations of the Ministry of Internal Affairs of Russia
Authors: Tikhonova Elena Aleksandrovna 
Published: January 2024

The subject of the study is the psychological resources of the senior staff of departments and faculties of educational organizations of the Ministry of Internal Affairs of Russia. The purpose of the research is to study the relationship between subjective representations of the success and effectiveness of the professional activities of managers and their resource availability. It was suggested that a large number of requirements imposed on the leadership of departments and faculties will actualize the psychological resources of the personality of managers with high efficiency of professional activity and lead to depletion of resources with low efficiency of professional activity. The study involved 376 heads and deputy heads of departments and faculties of educational organizations of the Ministry of Internal Affairs of Russia with service experience in the internal affairs bodies from 2 to 38 years, in office from several months to 29 years. In order to collect empirical data, the following methods were used: author's questionnaire; questionnaire "Strategies for overcoming stressful situations" by S. E. Hobfoll; "Questionnaire of proactive coping behavior" by E. Greenglas, R. Schwarzer and S. Taubert; questionnaire of "Psychological capital" by A. Bakker; "Test of resilience" by S. Maddi; "Test of life orientations" by J. Crambo and L. Maholika. The results of the study indicate that the self-assessment of the professional effectiveness and success of the management of educational organizations of the Ministry of Internal Affairs of Russia reflects the level of its resource availability. In general, the results of the study indicate a high resource availability of the senior staff of departments and faculties of educational organizations of the Ministry of Internal Affairs of Russia. In addition, based on subjective representations of the professional effectiveness and success of the leaders of this group, it is possible to predict the choice of constructive and proactive coping strategies that act as psychological resources of the individual: the higher the head evaluates the effectiveness of his professional activity, the more often he implements constructive and proactive coping strategies. The most significant contribution of the study is that its results can be used for an operational assessment of the actual resource availability of the head when conducting group and/or individual psychological support work by a psychologist.
Assessment of Protective Roles of Aqueous Seed Extract of Sour-Sop (Annona muricata) in Cerebellum Following Cadmium-induced Neurotoxicity of Adult Wistar Rats
Authors: Fakunle P. B.  , Samuel Ehiremen , Kayode Odubela , Kehinde Bamgbose 
Published: January 2024

Cadmium, a deleterious heavy metal that pervades the environment, has the potential to accumulate in the body and cause health complications. In the domain of traditional medicine, medicinal plants have been employed to combat the toxicity of heavy metals and treating of many diseases in traditional medicine.
Aim: To access the protective impact of Annona muricata seed extract on cadmium-triggered neurotoxicity in the cerebellum of albino wistarrat.
Place of Study: Department of Anatomy, Faculty of Basic Medical Sciences Olabisi Onabanjo University, Ogun State between June 2022 and June 2023
METHODOLOGY: After a period of 14 days of acclimatization, 40 healthy male wistar rats were randomly allocated to four groups, group1(2ml/kg of distilled water), group2 cadmium only(2g/kg SC), group3 (2g/kg cadmium + 100mg/kg extract of Annona muricata) and group4(2g/kg cadmium + 200mg/kg extract of Annona muricata) The rats were subjected to cadmium subcutaneously followed by the oral administration of aqueous seed extract of Annona Muricata for fifteen consecutive days.
Results: The impact of the extract on antioxidant enzymes activities of the cerebellum, cerebellar weight, as well as the histology of the cerebellum were scrutinized. The data revealed that the aqueous seed extract of Annona muricata, in a dose-dependent manner, augmented the levels of superoxide dismutase (SOD) (P=0.005) and catalase (CAT) (P=0.027) in contrast to the cadmium-only group. The relative weight of the cerebellum exhibited a significant increasein the treated groups compared to the cadmium-only group.
The histology of the cerebellum delineated pathological changes arising from the exposure to cadmium, while Annona muricata brought about regenerative changes.
Conclusion: In summary, the study posits that owing to the presence of phytochemicals in Annona muricata aqueous seed extract, it was efficacious in mitigating the neurotoxicity induced by cadmium in the cerebellum of male rats.
Nutritional Knowledge, WASH Practices of Mothers and Their Impact on the Nutritional Status of Children Aged 6–59 Months in Cumilla District, Bangladesh
Authors: Hossain Md. Shahadoth , Akter Tasniya , Sadiq Md. Zafar As 
Published: January 2024

Introduction: Child malnutrition is a pressing global public health issue, impacting both immediate health and future development. Adequate nutrition is a crucial aspect of public health, with maternal nutritional knowledge playing a vital role in ensuring optimal growth for children. Insufficient awareness about nutrition, even in households with sufficient resources, can result in poor outcomes for children.
Aim: This study aimed to explore the contemporary status of maternal nutritional knowledge, water, sanitation, and hygiene (WASH) practices and their effect on the nutritional status of children aged 6–59 months in the Cumilla district of Bangladesh.
Methods: A community-based cross-sectional study was carried out on 386 mother-child (6-59 months) pairs from 7 villages of the Cumilla district, Bangladesh. The study was designed to assess nutritional knowledge, WASH practices of mothers and nutritional status of their children using structured questionnaire and anthropometric measures. Chi square test was performed to investigate the association between maternal nutritional knowledge, WASH practices, and the nutritional status of children.
Results: The study revealed, the level of nutritional knowledge was average (72.3%) among mothers. The prevalence of stunting, underweight, wasting and overweight were 38.6%, 16.9%, 11.9%, and 7.8% respectively. A strong relationship was found between nutritional knowledge, WASH practices of mothers and child’s stunting (p<0.05) respectively. Maternal nutritional knowledge also had a strong association with underweight where female child was more prone to underweight than its male counterpart (p<0.05). There was no significant association between maternal nutritional knowledge, WASH practices and wasting (p = 0.224, p = 0.328) respectively.
Conclusion: The study revealed elevated rates of stunting and overweight in children compared to the national average, while underweight prevalence was lower and wasting prevalence was similar. Therefore, it is crucial to tackle social and cultural issues and implement effective strategies to enhance maternal nutritional knowledge and WASH practices to mitigate malnutrition in the rural areas of Bangladesh.
Prevalence of Dental Caries in Permanent Second Molars in Teenagers Using ICDAS
Authors: Rithanya M. , Mathew Mebin George 
Published: January 2024

Introduction: Dental caries is a chronic disease of the tooth which is represented by demineralisation and destruction of the organic matter of the tooth. Dental caries are versatile, capable of affecting the crown portion of the tooth initially, and may affect the unprotected root surfaces later on. International Caries Detection and Assessment System (ICDAS) provides a systematic approach of reviewing the available clinical dental caries to evaluate the development of dental caries
Materials and Methods: This study is a single centered retrospective study in which the data was collected from a private dental college and hospital in Chennai, India. Patient details were collected from the hospital management system and a total of 1804 samples were collected. The data was tabulated using Excel and then statistically analysed using the latest version of SPSS software.
Results and Discussion: The prevalence of ICDAS score 3 in second molars is higher in 17 year old patients with 2.88% in maxillary right second molars, 3.33% in maxillary left second molars, 3.55% in mandibular left second molars and 3.71% in mandibular right second molars. The prevalence of dental caries in permanent second molar is 9.34%. It is seen that ICDAS score 3 is 8.71% prevalent followed by ICDAS score 5 which is 0.63%.
Conclusion: Within the limitations of the study, it was found that the prevalence of dental caries in permanent second molars is 9.34% with ICDAS score 3 being the most prevalent followed by ICDAS score 5 which is 0.63%.
Melatonin synthesis: A possible indicator of intolerance to shiftwork
Authors: Griefahn Barbara , Künemund Christa , Golka Klaus , Thier Ricarda , Degen Gisela 
Published: October 2002

AbstractHypothesisMelatonin synthesis, which is directly controlled by the central circadian pacemaker indicates the circadian phase better than rectal temperature.MethodsThirty four men (16–32 years, 7 morning, 13 neither, 14 evening types) performed a constant routine (24–26‐hr bedrest, < 30 lux, 18–20°C, hourly isocaloric diet). Salivary melatonin level was determined hourly and rectal temperature was continuously recorded.ResultsThe nadir of rectal temperature occurred 1.5 hr (P = 0.017), the onset of melatonin synthesis 3 hr earlier (P < 0.0001) in morning than in evening types. Morningness was not related to the quantitative but significantly to the temporal parameters, closer to those of melatonin than of rectal temperature.ConclusionsThe melatonin onset is a more reliable indicator of the diurnal type than the nadir of rectal temperature. As morningness has been associated with intolerance to shiftwork, melatonin profiling provides a suitable basis for the establishment of directed preventive measures. Am. J. Ind. Med. 42:427–436, 2002. © 2002 Wiley‐Liss, Inc.
Micelle‐bound conformation of a hairpin‐forming peptide: Combined NMR and molecular dynamics study
Authors: Dixon Ann M. , Venable Richard M. , Pastor Richard W. , Bull T. E. 
Published: October 2002

AbstractA peptide fragment from a protein hairpin turn region was modified by addition of isoleucine residues to both ends to enhance binding to lipid micelles; the resulting peptide (I1‐I2‐C3‐N4‐N5‐P6‐H7‐I8‐I9) contains the core sequence I‐C‐N‐N‐P‐H from an antibody‐binding region of hemagglutinin A. Nuclear magnetic resonance (NMR) diffusion measurements indicated partial binding (43–65%) of the peptide to micelles of n‐octylglucoside and significantly stronger binding (85%) to dodecylphosphocholine (DPC) micelles. Simulated annealing and conformational analysis using nuclear Overhauser enhancement restraints revealed a type I or III hairpin turn between residues N5 and I8 of the DPC‐bound peptide. Amide exchange experiments support the possibility that a hydrogen bond forms between N5 and I8, stabilizing the turn. In contrast, no discernable structure was observed for the peptide in aqueous solution by either NMR or circular dichroism. Molecular dynamics simulations of DPC micelles and peptide–micelle complexes suggested that the peptide lies flat on the micelle surface and showed rapid rearrangement of the lipids to accommodate the bound peptide. According to a search performed using the basic local alignment search tool (BLAST), the sequences N‐P‐H‐I and N‐P‐H‐V are present as hairpin turns in eight of the nine proteins whose crystal structures were available. The addition of isoleucine residues and the use of lipid micelles to stabilize hairpin conformations equivalent to those found in proteins generates new possibilities for reproducing biologically important hairpin turns from short, linear peptides. Published 2002 Wiley Periodicals, Inc. Biopolymers 65: 284–298, 2002
Molecular cytogenetic analysis of genomic instability at the 1q12–22 chromosomal site in B‐cell non‐Hodgkin lymphoma
Authors: Itoyama Takahiro , Nanjungud Gouri , Chen Weiyi , Dyomin Vadim G. , Teruya‐Feldstein Julie , Jhanwar Suresh C. , Zelenetz Andrew D. , Chaganti R.S.K. 
Published: October 2002

AbstractAbnormalities of chromosome arm 1q have frequently been reported in B‐cell non‐Hodgkin lymphoma (NHL), and correlated with poor outcome. Five genes mapped to this region (BCL9, MUC1, FCGR2B, IRTA1, and RTA2) have been shown to be deregulated by juxtaposition with the IG genes. However, abnormalities of the 1q21–22 region that are not involved in translocations with the IG genes have not been addressed. We performed a molecular cytogenetic analysis of 1q12–22 abnormalities in 24 B‐cell NHL cases. The cases analyzed were in two groups: one, composed of 18 cases with the single break in the 1q12–22 region, and another, composed of six cases with multiple breaks in the 1q12–22 region. The involvement of heterochromatin and its vicinity was observed most frequently in the single‐break cases (13 of 18 cases). In this group, the recurring partner region was 1q32, which resulted in dup(1)(q12‐21q32) or trp(1)(q12q32) in 5 cases. The 6 cases with multiple breaks showed an unexpected level of instability along with complex combinations of abnormalities, especially sequential duplication and inversion, in the 1q12–22 region. The BCL9 locus was deleted by complex aberration in 2 of 6 cases. High‐level amplification of the WI‐16757 locus was found in 2 cases. Our studies demonstrate a high level of instability of the 1q12–22 region, possibly stemming from its chromatin organization. Chromosome arm 1q is gene‐rich, and characterization of aberrations described in this study can be expected to lead to the discovery of additional functionally significant genetic changes. © 2002 Wiley‐Liss, Inc.
Frequent amplification of 8q24, 11q, 17q, and 20q‐specific genes in pancreatic cancer
Authors: Mahlamäki Eija H. , Bärlund Maarit , Tanner Minna , Gorunova Ludmila , Höglund Mattias , Karhu Ritva , Kallioniemi Anne 
Published: October 2002

AbstractGenetic changes involved in the development and progression of pancreatic cancer are still partly unknown, despite the progress in recent years. In this study, comparative genomic hybridization analysis in 31 pancreatic cancer cell lines showed that chromosome arms 8q, 11q, 17q, and 20q are frequently gained in this tumor type. Copy number analysis of selected genes from these chromosome arms by fluorescence in situ hybridization showed amplification of the MYC oncogene in 54% of the cell lines, whereas CCND1 was amplified in 28%. In the 17q arm, the ERBB2 oncogene was amplified in 20% of the cell lines, TBX2 in 50%, and BIRC5 in 58%, indicating increased involvement toward the q telomere of chromosome 17. In the 20q arm, the amplification frequencies varied from 32% to 83%, with the CTSZ gene at 20q13 being most frequently affected. These results illustrate that amplification of genes from the 8q, 11q, 17q, and 20q chromosome arms is common in pancreatic cancer. © 2002 Wiley‐Liss, Inc.
Somatic von Hippel–Lindau disease gene mutation in clear‐cell renal carcinomas associated with end‐stage renal disease/acquired cystic disease of the kidney
Authors: Yoshida Minoru , Yao Masahiro , Ishikawa Isao , Kishida Takeshi , Nagashima Yoji , Kondo Kei‐ichi , Nakaigawa Noboru , Hosaka Masahiko 
Published: October 2002

AbstractIt has been documented that renal cell carcinomas (RCCs) occur frequently in patients treated with long‐term dialysis, especially in cases of end‐stage renal disease (ESRD)/acquired cystic disease of the kidney (ACDK). To address the molecular pathogenesis of ESRD/ACDK‐associated RCCs, we examined 14 RCCs (7 clear‐cell and 7 papillary carcinomas) in patients receiving dialysis for somatic mutations of the von Hippel–Lindau disease (VHL) gene as well as of the tyrosine kinase domain of the MET oncogene. Direct sequencing analyses revealed that three tumors exhibited VHL frameshifts (618delA, 386–395del10‐bp, and 723–724insTC). One of the VHL mutated tumors showed additional loss of heterozygosity at the VHL gene locus. Histopathologic and clinical data demonstrated that the three tumors having VHL mutations were clear‐cell RCCs occurring in ESRD with 55, 106, and 156 months of dialysis history, respectively. We did not find any tumors with mutations in the tyrosine kinase domain of the MET. These results demonstrated that the VHL tumor‐suppressor gene is also involved in a subset of clear‐cell RCCs occurring in ESRD/ACDK, as in the case of sporadic clear‐cell RCCs. However, mutations of the MET oncogene could not be found in the seven ESRD/ACDK‐associated papillary RCCs examined. © 2003 Wiley‐Liss, Inc.
Molecular genetic characterization of the EWS/CHN and RBP56/CHN fusion genes in extraskeletal myxoid chondrosarcoma
Authors: Panagopoulos Ioannis , Mertens Fredrik , Isaksson Margareth , Domanski Henryk A. , Brosjö Otte , Heim Sverre , Bjerkehagen Bodil , Sciot Raf , Dal Cin Paola , Fletcher Jonathan A. , Fletcher Christopher D. M. , Mandahl Nils 
Published: October 2002

AbstractExtraskeletal myxoid chondrosarcoma (EMC) is a soft‐tissue neoplasm cytogenetically characterized by the translocations t(9;22)(q22;q11–12) or t(9;17)(q22;q11), generating EWS/CHN or RBP56/CHN fusion genes, respectively. In the present study, 18 EMCs were studied both cytogenetically and at the molecular level. Chromosomal aberrations were detected in 16 samples: 13 with involvement of 9q22 and 22q11–12, and three with rearrangements of 9q22 and 17q11. Fifteen cases had an EWS/CHN fusion transcript and three had an RBP56/CHN transcript. The most frequent EWS/CHN transcript (type 1; 10 tumors), involved fusion of EWS exon 12 with CHN exon 3, and the second most common (type 5; two cases) was fusion of EWS exon 13 with CHN exon 3. In all tumors with RBP56/CHN fusion, exon 6 of RBP56 was fused to exon 3 of CHN. By genomic XL PCR and sequence analyses, the breakpoints from 14 cases were mapped in the EWS, RBP56, and CHN genes. In CHN, 12 breakpoints were found in intron 2 and only two in intron 1. In EWS, the breaks occurred in introns 7 (one break), 12 (eight breaks), and 13 (one break), and in RBP56 in intron 6. Repetitive elements such as Alu and LINE sequences seem to have limited, if any, importance in the genesis of EWS/CHN and RBP56/CHN chimeras. Furthermore, there were no chi, chi‐like, topoisomerase II, or translin consensus sequences in the introns harboring the translocation breakpoints, nor could the number of topo I sites in EWS, RBP56, and CHN introns explain the uneven distribution of the breakpoints among EWS or CHN introns. Additional genetic events, such as nucleotide insertions, homologies at the junction, deletions, duplications, and inversions, were found to accompany the translocations, indicating that the chromosomal translocations do not require sequence‐specific recombinases or extensive homology between the recombined sequences. © 2002 Wiley‐Liss, Inc.
Cell lines in inner ear research
Authors: Rivolta Marcelo N. , Holley Matthew C. 
Published: October 2002

AbstractCell lines have provided important experimental tools that have enhanced our understanding of neural and sensory function. They are particularly valuable in inner ear research because the auditory and vestibular systems are small, complex, and encased in several layers of bone. Organotypic cultures provide an invaluable experimental resource but require repeated microdissection and culture, and remain complex in terms of cell types and states of differentiation. A number of laboratories have established cell lines that offer a range of potential applications to hearing research. This review describes the advances that have already been made with these lines and the potential applications that they offer in the future. The majority of the cell lines are immortalized with a conditionally expressed, temperature sensitive variant of the SV40 tumor antigen. We discuss the value of these cells in developmental studies. © 2002 Wiley Periodicals, Inc. J Neurobiol 53: 306–318, 2002
Towards a molecular understanding of drosophila hearing
Authors: Caldwell Jason C. , Eberl Daniel F. 
Published: October 2002

AbstractThe Drosophila auditory system is presented as a powerful new genetic model system for understanding the molecular aspects of development and physiology of hearing organs. The fly's ear resides in the antenna, with Johnston's organ serving as the mechanoreceptor. New approaches using electrophysiology and laser vibrometry have provided useful tools to apply to the study of mutations that disrupt hearing. The fundamental developmental processes that generate the peripheral nervous system are fairly well understood, although specific variations of these processes for chordotonal organs (CHO) and especially for Johnston's organ require more scrutiny. In contrast, even the fundamental physiologic workings of mechanosensitive systems are still poorly understood, but rapid recent progress is beginning to shed light. The identification and analysis of mutations that affect auditory function are summarized here, and prospects for the role of the Drosophila auditory system in understanding both insect and vertebrate hearing are discussed. © 2002 Wiley Periodicals, Inc. J Neurobiol 53: 172–189, 2002
Parallel and antiparallel dimers of magainin 2: their interaction with phospholipid membrane and antibacterial activity
Authors: Mukai Yasuhiro , Matsushita Yuko , Niidome Takuro , Hatekeyama Tomomitsu , Aoyagi Haruhiko 
Published: October 2002

AbstractMagainin 2 (M2) forms pores by associating with several other M2 molecules in lipid membranes and shows antibacterial activity. To examine the effect of M2 dimerization on biological activity and membrane interaction, parallel and antiparallel M2 dimers were prepared from two monomeric precursors. Antibacterial and haemolytic activities were enhanced by dimerization. CD measurements showed that both dimers and monomers have an α‐helical structure in the presence of lipid vesicles. Tryptophan fluorescence shift and KI quenching studies showed that all the peptides were more deeply embedded in acidic liposomes than in neutral liposomes. Experiments on dye‐leakage activity and membrane translocation of peptides suggest that dimers and monomers form pores through lipid membranes, although the pore formation may be accompanied by membrane disturbance. Although dimerization of M2 increased the interaction activity with lipid membranes, no appreciable difference between the activities of parallel and antiparallel M2 dimers was observed. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.
In Vitro Pharmacodynamic Properties of Three Antifungal Agents against PreformedCandida albicansBiofilms Determined by Time-Kill Studies
Authors: Ramage Gordon , VandeWalle Kacy , Bachmann Stefano P. , Wickes Brian L. , López-Ribot José L. 
Published: October 2002

ABSTRACTWe have examined the in vitro activities of fluconazole, amphotericin B, and caspofungin againstCandida albicansbiofilms by time-kill methodology. Fluconazole was ineffective against biofilms. Killing of biofilm cells was suboptimal at therapeutic concentrations of amphotericin B. Caspofungin displayed the most effective pharmacokinetic properties, with ≥99% killing at physiological concentrations.