bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic insights into GABAergic development in Dravet Syndrome iPSC and therapeutic implications
Authors: Schuster Jens , Lu Xi , Dang Yonglong , Klar Joakim , Wenz Amelie , Dahl Niklas , Chen Xingqi 
Published: October 2023

AbstractDravet syndrome (DS) is a devastating early onset refractory epilepsy syndrome caused by variants in theSCN1Agene. A disturbed GABAergic interneuron function is implicated in the progression to DS but the underlying developmental and pathophysiological mechanisms remain elusive, in particularly at the chromatin level. In this study, we utilized induced pluripotent stem cells (iPSCs) derived from DS cases and healthy donors to model disease- associated epigenetic abnormalities of GABAergic development. Employing the ATAC-Seq technique, we assessed chromatin accessibility at multiple time points (Day 0, Day 19, Day 35, and Day 65) of GABAergic differentiation. Additionally, we elucidated the effects of the commonly used anti-seizure drug valproic acid (VPA) on chromatin accessibility in GABAergic cells. The distinct dynamics in chromatin profile of DS iPSC predicted accelerated early GABAergic development, evident at D19, and diverged further from the pattern in control iPSC with continued differentiation, indicating a disrupted GABAergic maturation. Exposure to VPA at D65 reshaped the chromatin landscape at a variable extent in different iPSC-lines and rescued the observed dysfunctional development in some DS iPSC-GABA. This study provides the first comprehensive investigation on the chromatin landscape of GABAergic differentiation in DS-patient iPSC, offering valuable insights into the epigenetic dysregulations associated with interneuronal dysfunction in DS. Moreover, our detailed analysis of the chromatin changes induced by VPA in iPSC-GABA holds the potential to improve development of personalized and targeted anti-epileptic therapies.
Control of intestinal stemness and cell lineage by histone variant H2A.Z isoforms
Authors: Rispal Jérémie , Rives Clémence , Jouffret Virginie , Leoni Caroline , Dubois Louise , Chevillard-Briet Martine , Trouche Didier , Escaffit Fabrice 
Published: October 2023

AbstractThe histone variant H2A.Z plays important functions in the regulation of gene expression. In mammals, it is encoded by two genes, giving raise to two highly related isoforms named H2A.Z.1 and H2A.Z.2, which can have similar or antagonistic functions depending on the promoter. Knowledge of the physiopathological consequences of such functions emerges, but how the balance between these isoforms regulates tissue homeostasis is not fully understood. Here, we investigated the relative role of H2A.Z isoforms in intestinal epithelial homeostasis. Through genome-wide analysis of H2A.Z genomic localization in differentiating Caco-2 cells, we uncovered an enrichment of H2A.Z isoforms on the bodies of genes which are induced during enterocyte differentiation, stressing the potential importance of H2A.Z isoforms dynamics in this process. Through a combination ofin vitroandin vivoexperiments, we further demonstrated the two isoforms cooperate for stem and progenitor cells proliferation, as well as for secretory lineage differentiation. However, we found that they antagonistically regulate enterocyte differentiation, with H2A.Z.1 preventing terminal differentiation and H2A.Z.2 favoring it. Altogether, these data indicate that H2A.Z isoforms are critical regulators of intestine homeostasis and may provide a paradigm of how the balance between two isoforms of the same chromatin structural protein can control physiopathological processes.
Mapping energy landscapes of homopolymeric RNAs via simulated tempering and deep unsupervised learning
Authors: Ramachandran Vysakh , Potoyan Davit A 
Published: October 2023

ABSTRACTConformational dynamics plays crucial roles in RNA functions about sensing and responding to environmental signals. The liquid-liquid phase separation of RNAs and the formation of stress granules partly relies on RNA’s conformational plasticity and its ability to engage in multivalent interactions. Recent experiments with homopolymeric and low-complexity RNAs have revealed significant differences in phase separations due to differences in base chemistry of RNA units. We hypothesize that differences in RNA phase-transition dynamics can be traced back to the differences in conformational dynamics of single RNA chains. In the present contribution, we utilize atomistic simulations with numerous unsupervised learning to map temperature dependence conformational free energy landscapes for homopolymeric RNA chains. These landscapes reveal a variety of metastable excited states influenced by the nature of base chemistry. We shed light on the distinct contributions of the polyphosphate backbone versus base chemistry in shaping conformational ensembles of different RNAs. We demonstrate that the experimentally observed temperature-driven shifts in metastable state populations align with experimental phase diagrams for homopolymeric RNAs. The work establishes a microscopic framework to reason about base-specific RNA propensity for phase separation. We believe our work will be valuable for designing novel RNA sensors for biological and synthetic applications.
Towards an “Evaluation Dilemmas Model” – designing an evaluation scheme for a European capital of culture
Authors: Degn Hans-Peter , Hadley Steven , Hansen Louise Ejgod 
Published: October 2023

PurposeDuring the evaluation of European Capital of Culture (ECoC) Aarhus 2017, the evaluation organisation rethinkIMPACTS 2017 formulated a set of “dilemmas” capturing the main challenges arising during the design of the ECoC evaluation. This functioned as a framework for the evaluation process. This paper aims to present and discuss the relevance of the “Evaluation Dilemmas Model” as subsequently applied to the Galway 2020 ECoC programme evaluation.Design/methodology/approachThe paper takes an empirical approach including auto-ethnography and interview data to document and map the dilemmas involved in undertaking an evaluation in two different European cities. Evolved via a process of practice-based research, the article addresses the development of and the arguments for the dilemmas model and considers its potential for wider applicability in the evaluation of large-scale cultural projects.FindingsThe authors conclude that the “Evaluation Dilemmas Model” is a valuable heuristic for considering the endogenous and exogenous issues in cultural evaluation.Practical implicationsThe model developed is useful for a wide range of cultural evaluation processes including – but not limited to – European Capitals of Culture.Originality/valueWhat has not been addressed in the academic literature is the process of evaluating ECoCs; especially how evaluators often take part in an overall process that is not just about the evaluation but also planning and delivering a project that includes stakeholder management and the development of evaluation criteria, design and methods.
Healthy employees are assets: a structural model based on individual and organizational characteristics for hotel employee well-being
Authors: Majeed Fatima , Ul haq Junaid 
Published: October 2023

PurposeHealthy employees are assets for organizations, especially in service organizations. This study considered variables that lead to psychological, life and workplace well-being for hotel employees. A comprehensive model is proposed for which Job Demand–Resource (JD-R) theory provides theoretical support.Design/methodology/approachData from four hundred and five hotel employees were collected to observe the individual and organizational factors that directly and indirectly result in three types of well-being: workplace, psychological and life.FindingsResults provide significant positive direct and indirect effects of individual characteristics (personal cynicism and job stress) on psychological, life and workplace well-being. Similarly, organizational characteristics (organizational values and job characteristics) showed positive direct and indirect effects on life and workplace well-being.Originality/valueThe findings provide a strong recommendation for managers to focus on individual- and organizational-level factors that enhance the well-being of employees.
Leading early childhood education centers as professional bureaucracies – social organizational structures in Finnish early childhood education
Authors: Roponen Hannele , Fonsén Elina , Ukkonen-Mikkola Tuulikki , Ahtiainen Raisa 
Published: October 2023

PurposeThis study examines the social organizational structure of one early childhood education (ECE) center in Finland and the relationship between this structure and the roles and the responsibilities of the members of the organization.Design/methodology/approachThe research is a qualitative case study with ethnographic features. Its variables for content analysis are based on Henry Mintzberg's theory of organizational design.FindingsThe study's results show that the organizational structure of the ECE center follows the organizational configuration of a Professional bureaucracy and that the multiprofessional teams follow the configuration of a Simple structure. The structures for centralization and decentralization are suitable for a professional bureaucracy, but the roles of the members of the organization and the processes for shared decision-making lack clarification. The shortage of qualified ECE teachers disrupts the function of the organization and the work of ECE leaders.Research limitations/implicationsThe educational background of subjects may have affected the findings.Originality/valueThe study uses Henry Mintzberg's organizational structure theory to evaluate how and why power is distributed and activities are coordinated at the ECE center. The results also show what parts of the organization pose challenges that most commonly disrupt the organization's operations. With these findings, it is possible to expand the understanding of roles and responsibilities in the currently reforming ECE environment and what ECE centers need to function effectively. The study is part of a larger research project and will be continued to examine the leadership culture of the ECE center.
The online entrepreneurship program effect on young people's self-efficacy and intention: an experiment in Argentina, Belgium, Bulgaria, China and Romania
Authors: Vankov Daniel , Kozma David , Vankov Borislav , Chiers Johan , Galanternik Martin , Wang Lin 
Published: October 2023

Purpose
Entrepreneurship can help tackle economic problems, such as unemployment. It is often promoted through education programs. There is a limited comprehensive and rigorous understanding of how entrepreneurship education programs and their ubiquitous distance delivery affect young people's entrepreneurial self-efficacy and intention, particularly in non-formal settings. The purpose of this study is to address this gap.

Design/methodology/approach
Underpinned by the Social Cognitive Theory, this paper investigates the effects of one entrepreneurship education program in a study with 145 young people from five countries aged 18 to 25 years, 62 Intervention and 83 Control participants. The program's impact on the participants' entrepreneurial intention and self-efficacy (across six sub-dimensions) was assessed in a one-off two-week quasi-experiment. Ex-ante and ex-post self-reported data were collected about the participants.

Findings
One-way analyses of covariance were performed to assess separately for changes in the Intervention participants' answers, relative to the Control group. The results of this study suggest the program significantly affected all measures.

Originality/value
These findings contribute to the discussions on the education programs’ effectiveness in promoting entrepreneurship. As a result, they may contribute to entrepreneurship education overcoming geographical and socio-economic hurdles (cost, time and entry barriers) to advance the development of industry, economy and community worldwide.
Governance mechanisms, firm performance and CEO compensation: evidence from Jordan
Authors: Alfawareh Faraj Salman , Che Johari Edie Erman , Ooi Chai-Aun 
Published: October 2023

Purpose
This paper aims to investigate the effect of governance mechanisms and firm performance on chief executive officer (CEO) compensation in relation to the Jordanian business environment. This study also examines the moderating role of gender diversity.

Design/methodology/approach
The sample is drawn from the annual reports of 68 Jordanian firms between 2015 and 2019. This paper uses the ordinary least square regression. It also uses the generalised method of moments approach to control any endogeneity issue and analyses the data in depth. In addition, it uses a dynamic model to address concerns regarding causality in the study’s models.

Findings
The results show that governance mechanisms and firm performance have an impact on CEO compensation. Furthermore, the outcomes indicate that gender diversity significantly and positively moderates the association between firm performance and CEO compensation. These findings enhance and support agency theory in the context of Jordan.

Practical implications
The study’s results have significant implications for policymakers, shareholders, investors, academicians and the public in the developing Jordanian market. The findings also support more monitoring and inspection to prevent the occurrence of opportunistic management behaviour and ensure that CEO remuneration packages are appropriately designed.

Originality/value
This study provides a unique understanding by explaining the impact of governance and performance on CEO compensation in a developing country such as Jordan. Besides that, the current study extends prior studies in Jordan significantly.
The more open, the better? Research on the influence of subject diversity on trust of tourism platforms
Authors: Xu Siyuan , Mou Yupeng , Ding Zhihua 
Published: October 2023

PurposeThe continuous impact of the pandemic and the downturn of the global economy have brought new challenges to the tourism industry. In this context, effectively attracting consumers and improving user stickiness are the top priorities of tourism platform companies. This study explores the impact of ethical concerns raised by new issues under the multi-governance environment on user stickiness. Based on the trust theory, the authors provide solutions for tourism platforms.Design/methodology/approachThis study adopted a quantitative approach, gathering survey data via an online platform. A total of 400 participants were investigated, and 356 valid questionnaires were returned, with a recovery rate of 89%. Questionnaires that did not meet the inclusion criteria were excluded, leaving 298 valid responses.FindingsStudies have found that consumers' ethical concerns about platform companies are key factors affecting user stickiness, and among these, consumer trust plays a mediating role. They have found that corporate social responsibility (CSR) behaviours help alleviate ethical concerns and improve trust in enterprises. At the same time, enterprises should properly control the number of platform collaborators, and excessive platform cooperation negatively moderates the impact of consumer ethical concerns on competence-based trust.Originality/valueThis study complements the deficiency of previous research with regard to ethical concerns in a multi-governance environment. These findings indicate that subject diversity exacerbates the negative impact of ethical concerns on consumer trust; however, CSR alleviates the impact of ethical concerns on consumer trust.
Mental well-being through HR analytics: investigating an employee supportive framework
Authors: Lathabhavan Remya 
Published: October 2023

PurposeOrganisations are increasingly adopting and adapting to technological advancements to stay relevant in the era of intense competition. Simultaneously, employee mental well-being has become a prominent global concern affecting people across various demographics. With this in mind, the present study explores the influence of human resource (HR) analytics, mental health organisational evidence-based management (OEBM) and organisational mental health support on the mental well-being of employees. Additionally, the study examines the moderating effects of manager and peer support on the association between organisational mental health support and the mental well-being of employees.Design/methodology/approachData were collected from 418 employees in India and structural equation modelling was performed to analyse the data.FindingsThe study found significant positive associations between HR analytics with mental health OEBM, organisational mental health support and mental well-being. Mental health OEBM was also found to be positively related to organisational mental health support and mental well-being. The moderating roles of manager and team support were also found to be significant in the associations between organisational mental health support and well-being.Originality/valueThe study showed that HR analytics is a valuable source of mental health data. This data can facilitate the development of evidence-based management (EBM) strategies to promote the mental well-being of employees.
Deep dermatophytosis caused by Trichophyton rubrum in an elderly patient with CARD9 deficiency: A case report and literature review
Authors: Ansai Osamu , Hayashi Ryota , Nakamura Anna , Sasaki Jin , Hasegawa Akito , Deguchi Tokiko , Yuki Akihiko , Oike Naoki , Ariizumi Takashi , Abe Masahiro , Miyazaki Yoshitsugu , Takenouchi Tatsuya , Kawashima Hiroyuki , Abe Riichiro 
Published: October 2023

AbstractDeep dermatophytosis is an invasive and sometimes life‐threatening fungal infection mainly reported in immunocompromised patients. However, a caspase recruitment domain‐containing protein 9 (CARD9) deficiency has recently been reported to cause deep dermatophytosis. Herein, we report the first Japanese case of deep dermatophytosis associated with CARD9 deficiency. An 80‐year‐old Japanese man with tinea corporis presented with subcutaneous nodules on his left sole. Histopathological findings revealed marked epithelioid cell granulomas with filamentous fungal structures in the deep dermis and subcutis, and the patient was diagnosed with deep dermatophytosis. Despite antifungal therapy, the subcutaneous nodule on his left sole gradually enlarged, his left calcaneal bone was invaded, and the patient finally underwent amputation of his left leg. Genetic analysis revealed a homozygous CARD9 c.586 A > G (p. Lys196Glu) variant, suggesting a CARD9 deficiency. Here, we discuss the clinical features of CARD9 deficiency–associated deep dermatophytosis with a case report and review of the literature.
‘For Pretty Frocks’: Upper Class Female Consumerism and the Criminality of Abortions in Newspaper Reports of the Uzielli Case 1898
Authors: Michael‐Berger Lee 
Published: October 2023

AbstractIn the spring of 1898, Emily Edith Uzielli, a married member of the London elite, underwent an abortion, which was illegal at the time. Mrs Uzielli died as a result of the procedure, and the abortionist, Dr Collins, was accused of murder. This article examines the interconnectivity between conceptions of femininity and ideas on consumerism and the criminality of abortions in late Victorian England. It demonstrates that contemporary discourses of gender and high‐powered consumption infiltrated and shaped the popular discourse of the criminality of abortions, which was depicted as closely linked to transgressions of domesticity. Through the exploration of press representations of the Uzielli case, I show that women's newly acquired liberty of promenading West End streets and shopping centres were paralleled with their alleged freedom to control family size, independently of their husbands, through the means of abortion. Abortion procuring was portrayed as yet another manifestation of such ‘feminine’ consumer practices.
‘Simply by collaborating’? The impact of public and nongovernmental sectors collaboration experience on its institutionalization in the Lithuanian crisis management system
Authors: Bortkevičiūtė Rasa 
Published: October 2023

AbstractDespite some evidence that the experience of collaboration contributes to its institutionalization in the crisis management system, the mechanisms behind this process remain unclear. Building on existing research, we distinguish six building blocks (i.e., pressure to collaborate, clarity of roles, mutual trust, leadership, positive feedback and learning) that influence the institutionalization of collaborative crisis management. By applying theory‐building process tracing to the Lithuanian COVID‐19 and irregular migration crises management, we analyse the interplay on these factors to derive the following propositions: (1) when crisis management involves partners with limited collaborative experience, both transformational and boundary spanning leadership are critical at different stages to institutionalize collaboration; (2) the clarification of roles, provision of positive feedback and the subsequent growth of trust experienced by partners engaged in collaborative activities contribute to the informal institutionalization of collaborative crisis management; (3) positive feedback on previous collaborative experience facilitates learning within the crisis management system and, when supported by the efforts of transformational leaders, leads to the formal institutionalization of collaborative crisis management. These propositions demonstrate how collaborative approaches can be fostered to deal with future crises as well as raise the question on the balance between formal and informal institutionalization of these practices.
A possible perfection
Authors: Theobald Simon 
Published: October 2023

AbstractAmong my interlocutors in Mashhad, Iran's second‐largest city, were individuals who repeatedly claimed that some persons, philosophies, and ethical lives not only might be but actually were perfect (kāmel). The salavāt, a polyvalent blessing upon the Prophet and his descendants, evinces this. By evoking a unified, authoritative, egalitarian moment, reoriented in praise of perfect religious exemplars, the salavāt provides a sonic mechanism that manifests a utopian public of Republican ambition. This acoustic form, and the perfect Islamist public that it creates, prompts an analysis that rethinks existing inquiry, which typifies moral lives as principally shaped by fracture. It calls instead for an examination that recognizes questions of ethics as potentially inflected by a utopianism, the legacies of which refract through societies for fleeting but powerfully sensed moments.
The impact of corporate environmental management practices on environmental performance
Authors: Hassan Omaima A. G. , Romilly Peter , Khadaroo Iqbal 
Published: October 2023

AbstractThis study draws on neo‐institutional theory to examine how and why corporate environmental management practices might affect environmental performance. It contributes to the literature by using a large, global data set to investigate the impact of 10 corporate environmental management practices on greenhouse gas emissions or emissions intensity. It focuses on greenhouse gas emissions which pose an existential threat to the people and planet, and the environmental management practices of corporations whose effectiveness has provoked cynicism and claims of “greenwash”. Our results are based on a dynamic, robust and large‐scale econometric analysis, which includes tests of association and Granger causation in comparison with earlier research. A key finding, which is of interest not only to the academic literature but also to policymakers and managers, is that environmental performance impacts environmental management practices but not vice versa, supporting the hypothesis that corporations adopt these practices as a symbolic legitimizing device rather than a genuine attempt derived from moral obligation to reduce their greenhouse gases or carbon intensity.