bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic observations of enhanced oil recovery and associated changes at carbonate-brine and carbonate-petroleum interfaces
Authors: Qin Tianzhu , Fenter Paul , AlOtaibi Mohammed , Ayirala Subhash , Yousef Ali 
Published: October 2023

AbstractEnhanced oil recovery (EOR) from carbonates is obtained by injection of controlled ionic strength brines containing “active ions” (e.g., SO42−, Mg2+, Ca2+). It is generally believed that this occurs through the interaction of the active ions at the carbonate-brine interface (e.g., within a thin brine layer separating the petroleum and the carbonate phases). Here, in-situ observations show how one active ion, SO42−, alters behavior at the carbonate-petroleum interface. Displacement of petroleum from initially oil-wet carbonate rocks using brines with variable SO4 concentrations systematically changes oil recovery, in situ contact angles, and connectivity of the oil phase, confirming that the active ion alters interactions at the oil/brine/carbonate interface, as expected. Measurements of model calcite-fluid interfaces show that there is no measurable sorption of SO4 to carbonate-brine interfaces but reveals that the carbonate-petroleum interface is altered by previous exposure to SO4-containing brines. These results suggest that EOR in carbonates is controlled indirectly by active ions. We propose that this may be due to a reduced oleophilicity of the carbonate caused by chemical complexation between the active ion and petroleum’s acidic and basic functional groups. This mechanism explains how both anions and cations act as active ions for EOR in carbonates.
Effects of a lotion containing probiotic ferment lysate as the main functional ingredient on enhancing skin barrier: a randomized, self-control study
Authors: Cui Hongchang , Feng Congrui , Zhang Tao , Martínez-Ríos Verónica , Martorell Patricia , Tortajada Marta , Cheng Sidao , Cheng Shumin , Duan Zhi 
Published: October 2023

AbstractThere is an emergent need to develop functional cosmetic ingredients for the topical management of skin barrier function. This study aimed to investigate the efficacy of a lotion containing fermented lysates VHProbi® Mix R for enhancing the skin barrier. In vitro studies demonstrated that fermented cultures of both Lacticaseibacillus rhamnosus VHProbi® E06 (E06) and L. paracasei VHProbi® E12 (E12) had antioxidant capacity, showing promising scavenging capability for 2,2-diphenyl-1-picryl-hydrazyl. The antioxidant capacity of these strains was also demonstrated in the model of Caenorhabditis elegans. In addition, the fermented lysates of both E06 and E12 enhanced the proliferation of HaCaT cells and ameliorated the toxicity induced by Staphylococcus aureus ATCC 25923, hydrogen peroxide, and ultraviolet B radiation in the HaCaT cell models, which simulated the irritants that facial sensitive skin is exposed to. Subsequently, the ingredient VHProbi® Mix R was formulated using four kinds of fermented lysates: E06, E12, Lactiplantibacillus plantarum VHProbi® E15, and Lactobacillus helveticus VHProbi® Y21. A clinical study was conducted to investigate whether a lotion containing VHProbi® Mix R would be beneficial for people to enhance skin barrier. The participants were asked to use the investigational product for 30 days. Several indicators, including transepidermal water loss (TEWL), skin moisturization, and redness were measured at day 0 and day 30 using VISIA®-CR and CK®-MPA systems. Meanwhile, the burden of sensitive skin (BoSS) and self-assessment questionnaires were performed at baseline and endpoint of this study. The study data showed that at day 30, there was a significant decrease in TEWL (P < 0.01), redness measured by CK®-MPA (P < 0.01), and redness profile measured by VISIA®-CR compared with the baseline measurements. Skin moisturization had significantly increased after treatment with the lotion for 30 days. BoSS and self-assessment questionnaires also substantiated that the participants felt a markedly positive change in their sensitive skin. Hence, we hypothesize that applying the topical functional VHProbi® Mix R could confer effective benefits for people with sensitive skin and this represents a promising intervention for enhancing skin barrier.
Combination therapy of tyrosine kinase inhibitor sorafenib with the HSP90 inhibitor onalespib as a novel treatment regimen for thyroid cancer
Authors: Mortensen Anja Charlotte Lundgren , Berglund Hanna , Hariri Mehran , Papalanis Eleftherios , Malmberg Christer , Spiegelberg Diana 
Published: October 2023

AbstractThyroid cancer is the most common endocrine malignancy, affecting nearly 600,000 new patients worldwide. Treatment with the BRAF inhibitor sorafenib partially prolongs progression-free survival in thyroid cancer patients, but fails to improve overall survival. This study examines enhancing sorafenib efficacy by combination therapy with the novel HSP90 inhibitor onalespib. In vitro efficacy of sorafenib and onalespib monotherapy as well as in combination was assessed in papillary (PTC) and anaplastic (ATC) thyroid cancer cells using cell viability and colony formation assays. Migration potential was studied in wound healing assays. The in vivo efficacy of sorafenib and onalespib therapy was evaluated in mice bearing BHT-101 xenografts. Sorafenib in combination with onalespib significantly inhibited PTC and ATC cell proliferation, decreased metabolic activity and cancer cell migration. In addition, the drug combination approach significantly inhibited tumor growth in the xenograft model and prolonged the median survival. Our results suggest that combination therapy with sorafenib and onalespib could be used as a new therapeutic approach in the treatment of thyroid cancer, significantly improving the results obtained with sorafenib as monotherapy. This approach has the potential to reduce treatment adaptation while at the same time providing therapeutic anti-cancer benefits such as reducing tumor growth and metastatic potential.
Modified electrode decorated with silver as a novel non-enzymatic sensor for the determination of ammonium in water
Authors: Altahan Mahmoud Fatehy , Ali Asmaa Galal , Hathoot Abla Ahmed , Azzem Magdi Abdel 
Published: October 2023

AbstractAmmonium is an essential component of the nitrogen cycle, which is essential for nitrogen cycling in ecosystems. On the other hand, ammonium pollution in water poses a great threat to the ecosystem and human health. Accurate and timely determination of ammonium content is of great importance for environmental management and ensuring the safety of water supply. Here we report a highly sensitive electrochemical sensor for ammonium in water samples. The modified electrode is based on the incorporation of silver nitrate (AgNO3) into a carbon paste embedded with 1-aminoanthraquinone and supported by multi-walled carbon nanotubes, which are commercially available. A potential of 0.75 V is applied to the modified electrode, followed by activation in hydrochloric acid. The modified electrode was used for square wave voltammetry of ammonium in water in the potential range of − 0.4–0.2 V. The performance of ammonium analysis was determined in terms of square wave frequency, square wave amplitude and concentration of electrolyte solution (sodium sulphate). The calculation of the surface area according to the Randles–Sevcik equation resulted in the largest surface area for the Ag/pAAQ/MWCNTs/CPE. The modified electrode exhibited a linear range of 5–100 µM NH4+ in 0.1 M Na2SO4 with a detection limit of 0.03 µM NH4+ (3σ). In addition, the modified electrode showed high precision with an RSD value of 9.93% for 10 repeated measurements. No interfering effect was observed at twofold and tenfold additive concentrations of foreign ions. Good recoveries were obtained in the analysis of tap and mineral water after spiking with a concentration of ammonium ions.
Clinical implications of RAB13 expression in pan-cancer based on multi-databases integrative analysis
Authors: Zhang Xu-dong , Liu Zhong-yuan , Luo Kai , Wang Xiang-kun , Wang Mao-sen , Huang Shuai , Li Ren-feng 
Published: October 2023

AbstractWorldwide, cancer is a huge burden, and each year sees an increase in its incidence. RAB (Ras-related in brain) 13 is crucial for a number of tumor types. But more research on RAB13's tumor-related mechanism is still required. This study's goal was to investigate RAB13's function in human pan-cancer, and we have also preliminarily explored the relevant mechanisms. To investigate the differential expression, survival prognosis, immunological checkpoints, and pathological stage of RAB13 in human pan-cancer, respectively, databases of TIMER2.0, GEPIA 2, and UALCAN were employed. CBioPortal database was used to analyze the mutation level, meanwhile, PPI network was constructed based on STRING website. The putative functions of RAB13 in immunological infiltration were investigated using single sample gene set enrichment analysis (ssGSEA). The mechanism of RAB13 in hepatocellular cancer was also briefly investigated by us using gene set enrichment analysis (GSEA). RAB13 was differentially expressed in a number of different cancers, including liver hepatocellular carcinoma (LIHC), stomach adenocarcinoma (STAD), etc. Additionally, RAB13 overexpression in LGG and LIHC is associated with a worse prognosis, including overall survival (OS) and disease-free survival (DFS). Then, we observed that early in BLCA, BRAC, CHOL, ESCA, HNSC, KICH, KIRC, LIHC, LUAD, LUSC, and STAD, the level of RAB13 expression was raised. Next, we found that “amplification” was the most common mutation in RAB13. The expression of SLC39A1, JTB, SSR2, SNAPIN, and RHOC was strongly positively linked with RAB13, according to a correlation study. RAB13 favorably regulated B cell, CD8 + T cell, CD4 + T cell, macrophage, neutrophil, and dendritic cell in LIHC, according to immune infiltration analysis. Immune checkpoint study revealed a positive correlation between RAB13 expression and PD1, PDL1, and CTLA4 in LIHC. According to GSEA, RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc. RAB13 is a possible therapeutic target in LIHC and can be used as a prognostic marker.
Performance enhancement in clustering cooperative spectrum sensing for cognitive radio network using metaheuristic algorithm
Authors: Srivastava Vikas , Singh Parulpreet , Mahajan Shubham , Pandit Amit Kant , Alshamrani Ahmad M. , Abouhawwash Mohamed 
Published: October 2023

AbstractSpectrum sensing describes, whether the spectrum is occupied or empty. Main objective of cognitive radio network (CRN) is to increase probability of detection (Pd) and reduce probability of error (Pe) for energy consumption. To reduce energy consumption, probability of detection should be increased. In cooperative spectrum sensing (CSS), all secondary users (SU) transmit their data to fusion center (FC) for final measurement according to the status of primary user (PU). Cluster should be used to overcome this problem and improve performance. In the clustering technique, all SUs are grouped into clusters on the basis of their similarity. In cluster technique, SU transfers their data to cluster head (CH) and CH transfers their combined data to FC. This paper proposes the detection performance optimization of CRN with a machine learning-based metaheuristic algorithm using clustering CSS technique. This article presents a hybrid support vector machine (SVM) and Red Deer Algorithm (RDA) algorithm named Hybrid SVM–RDA to identify spectrum gaps. Algorithm proposed in this work outperforms the computational complexity, an issue reported with various conventional cluster techniques. The proposed algorithm increases the probability of detection (up to 99%) and decreases the probability of error (up to 1%) at different parameters.
The effect of circulating iron on barrier integrity of primary human endothelial cells
Authors: Madsen M. C. , Podieh F. , Overboom M. C. , Thijs A. , den Heijer M. , Hordijk P. L. 
Published: October 2023

AbstractIron is hypothesized to be one of the contributors to cardiovascular disease and its levels in the circulation may correlate with cardiovascular risk. The aim of this study is to investigate the mechanisms that underlie the effects of iron on the barrier function of primary human endothelium. We used Human Umbilical Vein Endothelial Cells (HUVEC) to investigate the effects of Fe3+ using electric cell-substrate impedance sensing, microscopy, western blot and immunofluorescence microscopy. Exposure to Fe3+ caused EC elongation and upregulation of stress-induced proteins. Analysis of barrier function showed a dose-dependent drop in endothelial integrity, which was accompanied by Reactive Oxygen Species (ROS) production and could partly be prevented by ROS scavengers. Inhibition of contractility by the ROCK inhibitor Y27632, showed even more effective rescue of barrier integrity. Using western blot, we detected an increase in expression of the small GTPase RhoB, an inducer of EC contraction, and a small decrease in VE-cadherin, suggestive for an iron-induced stress response. Co-stimulation by TNFα and iron, used to investigate the role of low-grade inflammation, revealed an additive, negative effect on barrier integrity, concomitant with an upregulation of pro-inflammatory markers ICAM-1 and RhoB. Iron induces a response in HUVEC that leads to endothelial activation and a pro-inflammatory state measured by loss of barrier integrity which can be reversed by ROS scavengers, combined with inhibition of contractility. These data suggest that ROS-mediated damage of the vascular endothelium could contribute to the increased cardiovascular risk which is associated with elevated levels of circulating iron.
Deconvolution reveals cell-type-specific transcriptomic changes in the aging mouse brain
Authors: Ren Yingxue , Wang Xue , Zhang Shuwen , Hu Hongru , Quicksall Zachary , Lee Sangderk , Morganti Josh M. , Johnson Lance A. , Asmann Yan W. , Zhao Na 
Published: October 2023

AbstractMounting evidence highlights the crucial role of aging in the pathogenesis of Alzheimer's disease (AD). We have previously explored human apoE-targeted replacement mice across different ages and identified distinct molecular pathways driven by aging. However, the specific contribution of different brain cell types to the gene modules underlying these pathways remained elusive. To bridge this knowledge gap, we employed a computational deconvolution approach to examine cell-type-specific gene expression profiles in major brain cell types, including astrocytes (AS), microglia (MG), oligodendroglia (OG), neurons (NEU), and vascular cells (VC). Our findings revealed that immune module genes were predominantly expressed in MG, OG, and VC. The lipid metabolism module genes were primarily expressed in AS, MG, and OG. The mitochondria module genes showed prominent expression in VC, and the synapse module genes were primarily expressed in NEU and VC. Furthermore, we identified intra- and inter-cell-type interactions among these module genes and validated their aging-associated expression changes using published single cell studies. Our study dissected bulk brain transcriptomics data at the cellular level, providing a closer examination of the cell-type contributions to the molecular pathways driven by aging.
Autonomous AI systems in the face of liability, regulations and costs
Authors: Saenz Agustina D. , Harned Zach , Banerjee Oishi , Abràmoff Michael D. , Rajpurkar Pranav 
Published: October 2023

AbstractAutonomous AI systems in medicine promise improved outcomes but raise concerns about liability, regulation, and costs. With the advent of large-language models, which can understand and generate medical text, the urgency for addressing these concerns increases as they create opportunities for more sophisticated autonomous AI systems. This perspective explores the liability implications for physicians, hospitals, and creators of AI technology, as well as the evolving regulatory landscape and payment models. Physicians may be favored in malpractice cases if they follow rigorously validated AI recommendations. However, AI developers may face liability for failing to adhere to industry-standard best practices during development and implementation. The evolving regulatory landscape, led by the FDA, seeks to ensure transparency, evaluation, and real-world monitoring of AI systems, while payment models such as MPFS, NTAP, and commercial payers adapt to accommodate them. The widespread adoption of autonomous AI systems can potentially streamline workflows and allow doctors to concentrate on the human aspects of healthcare.
Dicalcin suppresses invasion and metastasis of mammalian ovarian cancer cells by regulating the ganglioside-Erk1/2 axis
Authors: Miwa Naofumi , Hanaue Mayu , Aoba Kayo , Saito Ryohei , Takamatsu Ken 
Published: October 2023

AbstractMetastasis, a multistep process including cancer cell migration and invasion, is the major cause of mortality in patients with cancer. Here, we investigated the effect of dicalcin, a Ca2+-binding protein, on the invasion and metastasis of ovarian cancer (OC) cells. Extracellularly administered dicalcin bound to the membrane of OV2944 cells, mouse OC cells, and suppressed their migration in vitro; however, cell viability or proliferation were unaffected. Repeated intraperitoneal injection of a partial peptide of dicalcin (P6) prolonged the survival, and reduced the number of microcolonies in the livers of cancer-bearing mice. P6 bound to the ganglioside GM1b in a solid-phase assay; treatment with P6 inhibited the constitutive activation of Erk1/2 in OC cells, whereas excess administration of GM1b augmented Erk activity and cancer cell migration in vitro. Thus, dicalcin, a novel suppressor of invasion and metastasis of OC cells, acts via the GM1b-Erk1/2 axis to regulate their migration.
A machine learning approach to rapidly project climate responses under a multitude of net-zero emission pathways
Authors: Kitsios Vassili , O’Kane Terence John , Newth David 
Published: October 2023

AbstractNavigating a path toward net-zero, requires the assessment of physical climate risks for a broad range of future economic scenarios, and their associated carbon concentration pathways. Climate models typically simulate a limited number of possible pathways, providing a small fraction of the data needed to quantify the physical risk. Here machine learning techniques are employed to rapidly and cheaply generate output mimicking these climate simulations. We refer to this approach as QuickClim, and use it here to reconstruct plausible climates for a multitude of concentration pathways. Higher mean temperatures are confirmed to coincide with higher end-of-century carbon concentrations. The climate variability uncertainty saturates earlier, in the mid-century, during the transition between current and future climates. For pathways converging to the same end-of-century concentration, the climate is sensitive to the choice of trajectory. In net-zero emission type pathways, this sensitivity is of comparable magnitude to the projected changes over the century.
Intra-Operative Blood Conservation During Cardiac Surgery
Authors: Vertrees Roger A. , Auvil Jackson , Rohrer Charlene , Rousou John H. , Engelman Richard M. 
Published: October 2023

The Haemonetics Cell Saver, a device developed for the recovery of autologous blood, was evaluated at our institution. The major areas of concern in evaluating this device were: quantity of blood salvaged, reduction in blood bank usage and a possible monetary savings to the patient.
This study consists of 33 consecutive adult patients undergoing cardiac procedures requiring systemic heparinization and cardiopulmonary bypass. Of these 33 adult patients, a group of 13 patients using autotransfusion was compared to a control group of 20 cardiac patients without autotransfusion.
In the group of 13 autotransfused patients, a mean of 6.6 units of bank blood was used throughout their entire hospital stay, as compared to 9.7 units of bank blood in the nontransfuscd group. This is a significant reduction in blood usage and, since the cost of the Haemonetics software is recovered with two units of blood, a reduced cost to the patient was realized.
A Technique For Infusion of Hypothermic Cardioplegic Solutions
Authors: Hurdle Michael , Djuric Michael , Mork Greg , Mammana Robert B. 
Published: October 2023

The infusion of a hypothermic cardioplegia solution has been accepted as a beneficial adjunct to routine open cardiac procedures.
Methods of cardioplegia infusion vary greatly in terms of efficiency, consistency, and ease of operation.
We have developed an infusion system which has proven safe, reliable, economical, and easily implemented. The system has been utilized in over 170 clinical cases with excellent results and will be described below.
Reducing the Rate of Premature Births through Early Diagnosis and Pregnancy-Adapted Treatment of Hypothyroidism
Authors: Torremante Pompilio , Berge Nils Kristian , Weiss Christel 
Published: October 2023

AbstractThe aim of this study was to determine the extent to which regular monitoring of maternal free thyroxine level and pregnancy-adapted L-thyroxine replacement therapy before and during
pregnancy in patients with existing or newly diagnosed latent and manifest hypothyroidism as well as hypothyroxinemia can influence the rate of premature births.This is a retrospective cohort study assessing 1440 pseudonymized survey questionnaires to evaluate the risks of premature birth with two study groups from the same medical practice, and
a nationally recruited control group. Study group A (n = 360) had already been taking L-thyroxine prior to conception, study group B (n = 580) started taking it after conception. Both
study groups had a maximum gestational age of 12 + 0 GW. In the study groups, TSH and free thyroxine levels were determined regularly for dose adjustment purposes. The aim was to keep the
free thyroxine level in the euthyroid hyperthyroxinemic range within the pregnancy adapted reference range. The control group (n = 500) had taken L-thyroxine during pregnancy according to
criteria that were not known, as the questionnaire did not include any questions regarding this matter. Taking other risk factors into account, the influence of pregnancy-adapted
L-thyroxine replacement therapy on the rate of premature births was determined using logistic regression analysis.Compared with the control group, the premature birth rate was 70% lower (p < 0.0001) in study group A and 42% lower in study group B (p = 0.0086), while the odds ratio, at 3.46, was
particularly significant in study group A. High blood pressure (odds ratio 5.21), body mass index per kg/m2 (odds ratio 0.91) and S. p. premature birth were identified as other
independent risk factors.The results show an association between more intensive thyroid diagnostics and pregnancy-adapted L-thyroxine replacement therapy and a decrease in premature births. Further studies should
be conducted to confirm these results.