bluuebunny/crossref_metadata_2025_split huggingface dataset:
A defined serum‐free culture system for human long‐term haematopoietic stem cells
Authors: Dong Yichao , Cai Ruikun , Fang Mingxia , Chen Yuqi , Li Peng , Guo Changlong , Ma Xu 
Published: December 2023

SummaryLong‐term repopulating haematopoietic stem cells (LT‐HSCs) have the ability to reconstitute the entire haematopoietic system following transplantation permanently. Despite great achievements in HSC transplantation, the limited transplantable HSC number, especially LT‐HSCs, remains critical for successful transplantation and broader applications. In this study, we established a defined serum‐free culture system for in vitro expansion of LT‐HSCs. This culture system (E1) expanded LT‐HSCs from umbilical cord blood, human mobilization peripheral blood and bone marrow. These E1‐expanded HSCs reconstituted the haematopoietic and immune systems in primary and secondary transplanted mice in a short time. Better haematopoietic reconstitution was observed in secondary xenografted mice. Moreover, we obtained the comprehensive expression profile and cellular components of LT‐HSCs from umbilical cord blood. Our study provides a valuable tool for LT‐HSC research and may improve clinical applications of HSCs.
Should oligomonocyticCMMLbe classified as an independent disease entity?
Authors: Zhao Helong Gary 
Published: December 2023

The 2022 WHO Classification revised the diagnostic criteria of CMML and included the variant, formerly known as oligomonocytic CMML (O‐CMML), into the CMML disease. CMML is currently subtyped into MD‐CMML and MP‐CMML. In a recent study, Castaño‐Díez et al. showed that the newly included O‐CMML has unique clinical features and is an independent favourable risk factor, and proposed to view O‐CMML as an independent subtype of CMML, parallel to MD‐CMML and MP‐CMML.Commentary on: Castaño‐Díez et al. Characteristics and long‐term outcome in a large series of chronic myelomonocytic leukaemia patients including 104 formerly referred to as oligomonocytic. Br J Haematol 2024;204:892‐897.
Off‐clamp vs on‐clamp robot‐assisted partial nephrectomy: a systematic review and meta‐analysis
Authors: Fong Khi Yung , Gan Valerie Huei Li , Lim Benjamin Jia Han , Chan Yiong Huak , Castellani Daniele , Chen Kenneth , Tay Kae Jack , Ho Henry Sun Sien , Yuen John Shyi Peng , Aslim Edwin , Teoh Jeremy , Lim Ee Jean 
Published: December 2023

ObjectiveTo compare intra‐ and postoperative outcomes between off‐clamp and on‐clamp robot‐assisted partial nephrectomy (RAPN), using data from randomised controlled trials (RCTs) or covariate‐matched studies (propensity score‐matched or matched‐pair analysis).MethodsA Preferred Reporting Items for Systematic Reviews and Meta‐Analyses‐compliant literature review was conducted on PubMed, EMBASE, Scopus and CENTRAL for relevant studies comparing off‐clamp to on‐clamp RAPN. Primary outcomes were estimated blood loss, postoperative percentage decrease in estimated glomerular filtration rate (eGFR), and margin positive rate. Secondary outcomes were operative time, postoperative eGFR, length of stay, all postoperative complications, major complications, and need for transfusion. Random‐effects meta‐analyses were performed to generate mean differences (MDs) or odds ratios (ORs).ResultsA total of 10 studies (2307 patients) were shortlisted for analysis. There was no significant difference in estimated operative blood loss between off‐clamp and on‐clamp RAPN (MD 21.9 mL, 95% confidence interval [CI] −0.9 to 44.7 mL; P = 0.06, I2 = 58%). Off‐clamp RAPN yielded a smaller postoperative eGFR deterioration (MD 3.10%, 95% CI 1.05–5.16%; P = 0.008, I2 = 13%) and lower odds of margin positivity (OR 0.62, 95% CI 0.40–0.94; P = 0.03, I2 = 0%). No significant differences were found for all secondary outcomes.ConclusionsOff‐clamp and on‐clamp RAPN are similarly effective approaches for selected renal masses. Within the classic trifecta of PN outcomes, off‐clamp RAPN yields similar rates of perioperative complications and may possibly offer better preservation of renal function and reduced margin‐positive rates.
Botulinum toxin type A ameliorates rat dorsal root ganglia neuron pyroptosis in postherpetic neuralgia by upregulating cathelicidin antimicrobial peptide to inhibit neutrophil elastase
Authors: Wan Quan 
Published: December 2023

AbstractBotulinum toxin type A (BoNT/A) has exhibited efficacy in postherpetic neuralgia (PHN) treatment, and this study aims to uncover its underlying mechanisms. Resiniferatoxin (RTX)‐induced PHN rats were given BoNT/A. Rat postoperative pain behaviors were assessed by Von Frey test. Cleaved‐synaptosomal protein 25 kDa (cl‐SNAP‐25) or cathelicidin antimicrobial peptide (CAMP) expression in rat dorsal root ganglia (DRG) was detected by immunofluorescence or immunohistochemistry. Healthy rat‐derived DRG neurons were transfected, incubated with lipopolysaccharides (LPS)/adenosine 5′‐triphosphate (ATP) to stimulate pyroptosis and treated with BoNT/A. The CCK‐8, Western blot, ELISA, and qRT‐PCR were used to assess the viability, levels of pyroptosis‐related proteins proinflammatory cytokine levels, as well as CAMP and ELANE mRNA levels. BoNT/A (30 U/kg) promoted cl‐SNAP‐25 expression in rat DRG and reversed RTX‐induced decrease of rat paw withdrawal thresholds and CAMP expression and increase of pyroptosis‐associated protein and inflammatory factor expression in rat DRG. CAMP interacted with ELANE in rat DRG neurons. BoNT/A attenuated LPS/ATP‐stimulated inhibition of viability and CAMP expression and upregulation of inflammatory mediators, pyroptosis‐related proteins, and ELANE expression in rat DRG neurons, which was counteracted by CAMP silencing. However, ELANE knockdown offset the effect of CAMP silencing in LPS/ATP/BoNT/A‐treated rat DRG neurons. On the whole, BoNT/A alleviates rat DRG neuron pyroptosis during PHN by upregulating CAMP to inhibit ELANE.
How parent–child brain‐to‐brain synchrony can inform the study of child development
Authors: Alonso Angelica , McDorman S. Alexa , Romeo Rachel R. 
Published: December 2023

AbstractIt is well established that parent–child dyadic synchrony (e.g., mutual emotions, behaviors) can support development across cognitive and socioemotional domains. The advent of simultaneous two‐brain hyperscanning (i.e., measuring the brain activity of two individuals at the same time) allows further insight into dyadic neural synchrony. In this article, we review 16 recent studies of naturalistic, parent–child brain‐to‐brain synchrony, finding relations with the nature of interactions (collaborative vs. competitive, parent vs. stranger), proximal social cues (gaze, affect, touch, and reciprocity), child‐level variables (irritability, self‐regulation), and environmental factors (parental stress, family cohesion, and adversity). We then discuss how neural synchrony may provide a biological mechanism for refining broader theories on the developmental benefits of dyadic synchrony. We also highlight critical areas for future study, including examining synchrony trajectories longitudinally, including more diverse participants and interaction contexts, and studying caregivers beyond mothers (e.g., other family members, teachers). We conclude that neural synchrony is an exciting and important window into understanding how caregiver–child dyadic synchrony supports children's social and cognitive development.
An automated, data‐driven approach to children's social dynamics in space and time
Authors: Horn Lisa , Karsai Márton , Markova Gabriela 
Published: December 2023

AbstractMost children first enter social groups of peers in preschool. In this context, children use movement as a social tool, resulting in distinctive proximity patterns in space and synchrony with others over time. However, the social implications of children's movements with peers in space and time are difficult to determine due to the difficulty of acquiring reliable data during natural interactions. In this article, we review research demonstrating that proximity and synchrony are important indicators of affiliation among preschoolers and highlight challenges in this line of research. We then argue for the advantages of using wearable sensor technology and machine learning analytics to quantify social movement. This technological and analytical advancement provides an unprecedented view of complex social interactions among preschoolers in natural settings, and can help integrate young children's movements with others in space and time into a coherent interaction framework.
Does marital satisfaction predict children aggression? Insights from parents' and children's psychological distress
Authors: Wang Zhiyou , Zhang Xinwen , Ye Yajun 
Published: December 2023

AbstractMarital satisfaction is a key indicator of family members' life satisfaction and mental and physical health. Parents' marital functioning affects their children's development. Given the significant impacts of marital satisfaction on children's psychological and behavioural development, the influencing paths need to be explored. This study aims to extend prior research by examining the direct effect of marital satisfaction on children's aggression and the intermediary roles of parents' and children's psychological distress. Using multi‐stage cluster random sampling, a total of 971 children and their parents were recruited in Shaanxi Province in Mainland China. Self‐reported questionnaires regarding marital satisfaction, psychological distress and aggression were completed. Results showed no significant direct impact of marital satisfaction on children's aggression, but children's psychological distress mediated this link. Moreover, parents' and children's psychological distress served as sequential mediators in the nexus between marital satisfaction and children's aggression. It means that a chain mediating mechanism existed. A better understanding of the underlying mechanisms may offer valuable insights that would aid policymakers and clinicians in designing effective prevention and interventions targeting children's behavioural problems.
Do markets Trump politics? Fossil and renewable market reactions to major political events
Authors: Mukanjari Samson , Sterner Thomas 
Published: December 2023

AbstractWe investigate the effects of three events with major importance for climate policy on energy sector stocks: the Paris Agreement, the Trump election and presidency, and the Biden election. By combining event studies with impulse‐indicator saturation methods, we show that the Paris Agreement and the election of Mr. Biden benefited renewable industries, while the election of Mr. Trump had negative effects. For fossil fuel industries, the effects were largely the opposite. Despite Trump's efforts to eliminate environmental regulations, his presidency did however witness a decrease in both US coal production and consumption, while natural gas and oil consumption increased.
Pupal colour–pupation substrate correlation in butterflies
Authors: Mayekar Harshad Vijay , Kodandaramaiah Ullasa 
Published: December 2023

AbstractThe pupal colour of many butterflies is plastic, being green or brown depending on the environment in which they develop. In most species with pupal colour plasticity (PCP), larvae that pupate on leaves tend to develop into green pupae whereas those that pupate off‐leaf (e.g., on the stem or soil) tend to become brown. This correlation between background and pupal colour has been hypothesized to protect the butterflies against predation by allowing them to match their background, although experimental evidence is scarce. Furthermore, not all butterflies exhibit PCP, and the extent to which substrate choice influences PCP remains unclear. We use a comparative approach involving multiple, closely related species varying in the degree of PCP to test predictions related to the correlation between pupal colour and pupation substrate. We studied PCP in response to larval density in five common satyrine species (Nymphalidae), two dimorphic and three monomorphic. As predicted, species with monomorphic green pupae preferred to pupate on leaves whereas those with monomorphic brown pupae preferred off‐leaf substrates. In species with dimorphic pupae, pupal colour correlated with pupation substrate. We also predicted that higher larval density induces a greater proportion of off‐leaf pupation in the dimorphic species, but not in the monomorphic species. However, this prediction was not supported. Overall, we found a strong across‐species correlation between pupation substrate choice and pupal colour plasticity, and our results support the idea that pupal colour plasticity can be an adaptation against predation.
Temperature dependence of competitive ability is cold‐shifted compared to that of growth rate in marine phytoplankton
Authors: Sunday Jennifer M. , Bernhardt Joey R. , Harley Christopher D. G. , O'Connor Mary I. 
Published: December 2023

AbstractThe effect of climate warming on community composition is expected to be contingent oncompetitiveoutcomes, yet approaches to projecting ecological outcomes often rely on measures of density‐independent performance across temperatures. Recent theory suggests that the temperature response of competitive ability differs in shape from that of population growth rate. Here, we test this hypothesis empirically and find thermal performance curves of competitive ability in aquatic microorganisms to be systematically left‐shifted and flatter compared to those of exponential growth rate. The minimum resource requirement for growth,R*—an inverse indicator of competitive ability—changes with temperature following a U‐shaped pattern in all four species tested, contrasting from their left‐skewed density‐independent growth rate thermal performance curves. Our results provide new evidence that exploitative competitive success is highest at temperatures that are sub‐optimal for growth, suggesting performance estimates of density‐independent variables might underpredict performance in cooler competitive environments.
Ways parental health and stress shape the parenting of preteens in the rural Ozark Mountains
Authors: Oerther Sarah , Papachrisanthou Michelle M. 
Published: December 2023

AbstractObjectiveWe explore how parental health and stress shape the rearing of preteen children in the rural Ozark Mountains.BackgroundIdentifying concerns that impact preteen parents' health and stress levels may facilitate understanding ways to promote resilience and happiness for families in underserved rural communities.MethodsUsing an interpretive phenomenological approach grounded in Lazarus's stress and coping paradigm, the current qualitative study used semistructured interviews with 20 White parents in the rural area of the Ozark Mountains who had at least one child born between 2008–2011.ResultsStudy participants' stories revealed that stressors related to parenting preteen children were rooted in stress from mental health or indirectly tied to social roles, such as occupational stress or financial constraints.ConclusionInterventions and tools need to be developed that are specific to the culture of rural areas and that focus on families as an essential part of developing resilience and happiness and mitigating the adverse effects of stress.ImplicationsInterventions focused on families need to include diverse social factors that impact family resilience and happiness and include education to increase health literacy, family supports related to improving connections between parents and their preteen children, family tax credits to reduce hardships for parents, a tool for screening parents for stress, and expansion of telehealth opportunities.
Effects of whole‐soil warming on CH4 and N2O fluxes in an alpine grassland
Authors: Chen Ying , Han Mengguang , Qin Wenkuan , Hou Yanhui , Zhang Zhenhua , Zhu Biao 
Published: December 2023

AbstractGlobal climate warming could affect the methane (CH4) and nitrous oxide (N2O) fluxes between soils and the atmosphere, but how CH4 and N2O fluxes respond to whole‐soil warming is unclear. Here, we for the first time investigated the effects of whole‐soil warming on CH4 and N2O fluxes in an alpine grassland ecosystem on the Tibetan Plateau, and also studied the effects of experimental warming on CH4 and N2O fluxes across terrestrial ecosystems through a global‐scale meta‐analysis. The whole‐soil warming (0–100 cm, +4°C) significantly elevated soil N2O emission by 101%, but had a minor effect on soil CH4 uptake. However, the meta‐analysis revealed that experimental warming did not significantly alter CH4 and N2O fluxes, and it may be that most field warming experiments could only heat the surface soils. Moreover, the warming‐induced higher plant litter and available N in soils may be the main reason for the higher N2O emission under whole‐soil warming in the alpine grassland. We need to pay more attention to the long‐term response of greenhouse gases (including CH4 and N2O fluxes) from different soil depths to whole‐soil warming over year‐round, which could help us more accurately assess and predict the ecosystem‐climate feedback under realistic warming scenarios in the future.
Climate and mineral accretion as drivers of mineral‐associated and particulate organic matter accumulation in tidal wetland soils
Authors: Fu Chuancheng , Li Yuan , Zeng Lin , Tu Chen , Wang Xiaoli , Ma Haiqing , Xiao Leilei , Christie Peter , Luo Yongming 
Published: December 2023

AbstractTidal wetlands sequester vast amounts of organic carbon (OC) and enhance soil accretion. The conservation and restoration of these ecosystems is becoming increasingly geared toward “blue” carbon sequestration while obtaining additional benefits, such as buffering sea‐level rise and enhancing biodiversity. However, the assessments of blue carbon sequestration focus primarily on bulk SOC inventories and often neglect OC fractions and their drivers; this limits our understanding of the mechanisms controlling OC storage and opportunities to enhance blue carbon sinks. Here, we determined mineral‐associated and particulate organic matter (MAOM and POM, respectively) in 99 surface soils and 40 soil cores collected from Chinese mangrove and saltmarsh habitats across a broad range of climates and accretion rates and showed how previously unrecognized mechanisms of climate and mineral accretion regulated MAOM and POM accumulation in tidal wetlands. MAOM concentrations (8.0 ± 5.7 g C kg−1) (±standard deviation) were significantly higher than POM concentrations (4.2 ± 5.7 g C kg−1) across the different soil depths and habitats. MAOM contributed over 51.6 ± 24.9% and 78.9 ± 19.0% to OC in mangrove and saltmarsh soils, respectively; both exhibited lower autochthonous contributions but higher contributions from terrestrial or marine sources than POM, which was derived primarily from autochthonous sources. Increased input of plant‐derived organic matter along the increased temperature and precipitation gradients significantly enriched the POM concentrations. In contrast, the MAOM concentrations depended on climate, which controlled the mineral reactivity and mineral–OC interactions, and on regional sedimentary processes that could redistribute the reactive minerals. Mineral accretion diluted the POM concentrations and potentially enhanced the MAOM concentrations depending on mineral composition and whether the mineral accretion benefited plant productivity. Therefore, management strategies should comprehensively consider regional climate while regulating sediment supply and mineral abundance with engineering solutions to tap the OC sink potential of tidal wetlands.
Deep learning to extract the meteorological by‐catch of wildlife cameras
Authors: Alison Jamie , Payne Stephanie , Alexander Jake M. , Bjorkman Anne D. , Clark Vincent Ralph , Gwate Onalenna , Huntsaar Maria , Iseli Evelin , Lenoir Jonathan , Mann Hjalte Mads Rosenstand , Steenhuisen Sandy‐Lynn , Høye Toke Thomas 
Published: December 2023

AbstractMicroclimate—proximal climatic variation at scales of metres and minutes—can exacerbate or mitigate the impacts of climate change on biodiversity. However, most microclimate studies are temperature centric, and do not consider meteorological factors such as sunshine, hail and snow. Meanwhile, remote cameras have become a primary tool to monitor wild plants and animals, even at micro‐scales, and deep learning tools rapidly convert images into ecological data. However, deep learning applications for wildlife imagery have focused exclusively on living subjects. Here, we identify an overlooked opportunity to extract latent, ecologically relevant meteorological information. We produce an annotated image dataset of micrometeorological conditions across 49 wildlife cameras in South Africa's Maloti‐Drakensberg and the Swiss Alps. We train ensemble deep learning models to classify conditions as overcast, sunshine, hail or snow. We achieve 91.7% accuracy on test cameras not seen during training. Furthermore, we show how effective accuracy is raised to 96% by disregarding 14.1% of classifications where ensemble member models did not reach a consensus. For two‐class weather classification (overcast vs. sunshine) in a novel location in Svalbard, Norway, we achieve 79.3% accuracy (93.9% consensus accuracy), outperforming a benchmark model from the computer vision literature (75.5% accuracy). Our model rapidly classifies sunshine, snow and hail in almost 2 million unlabelled images. Resulting micrometeorological data illustrated common seasonal patterns of summer hailstorms and autumn snowfalls across mountains in the northern and southern hemispheres. However, daily patterns of sunshine and shade diverged between sites, impacting daily temperature cycles. Crucially, we leverage micrometeorological data to demonstrate that (1) experimental warming using open‐top chambers shortens early snow events in autumn, and (2) image‐derived sunshine marginally outperforms sensor‐derived temperature when predicting bumblebee foraging. These methods generate novel micrometeorological variables in synchrony with biological recordings, enabling new insights from an increasingly global network of wildlife cameras.