bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of a three-level evaluation of the Dutch Healthy School program
Authors: Vennegoor Gerjanne , Vonk Lisanne , van Assema Patricia , Huijts Tim , Eekhout Iris , Molleman Gerard R. M. , Levels Mark , Jansen Maria W. J. 
Published: January 2024

AbstractAn increasing number of schools obtained a Healthy School certificate, yet little is known about factors, characteristics, and processes, i.e. conditions, influencing the effectiveness of the Healthy School program. Therefore, a mixed methods evaluation study will be conducted on three levels: the student, the school, and the region. The research questions are focused on: 1) conditions for student outcomes, 2) conditions for implementation in schools, and 3) conditions for regional support. For the evaluation, the school and region are considered complex adaptive systems. Conditions for intended outcomes on student lifestyle, health, and academic achievement will be examined using a combination of existing datasets including school codes. Based on student outcomes, best and worst performing schools will be selected for predominantly qualitative analysis to further explain differences. To get insight into conditions for implementation in schools and regional support in nine regions, the degree of implementation will be determined with a questionnaire to select schools for predominantly qualitative analysis. Combined, this study will not only provide insight into the current situation on the three levels, but also explain differences between regions, schools, and students. Consequently, it will be possible to provide concrete advice to strengthen implementation of the Healthy School program.
Novel integrative data for Indomalayan echiniscids (Heterotardigrada): new species and old problems
Authors: Gąsiorek Piotr , Michalczyk Łukasz 
Published: January 2024

AbstractThe Malay Archipelago comprises thousands of islands that house a variety of biomes, from tropical rainforests of Borneo, Sumatra and Celebes to the Lesser Sundas deciduous forests. In this paper, we present an extensive genetic and morphological dataset for the genus Kristenseniscus, demonstrating the presence of the walteri complex that contains several pseudocryptic species. One of them, Kristenseniscus exanthema sp. nov., is described from Ambon and Seram (the Moluccas). First genetic data are released for two potentially pantropical Echiniscus species: E. africanus Murray, 1907 and E. pusae Marcus, 1928. Furthermore, Echiniscus minutus sp. nov. (Sulawesi Tengah), exhibiting trunk spines and contrasting dorsal sculpturing that comprise both pores and epicuticular granules, is found to constitute a separate evolutionary lineage within the Echiniscus spinulosus morphogroup (epicuticular granules are typically absent in the spinulosus group). Based on the phylogenetic reconstructions involving ca. 80 spp. of echiniscids representing the Echiniscus evolutionary line, we hypothesise that the tropical and subtropical regions served as the main radiation zone for this heterotardigrade clade. Finally, Pseudechiniscus (Meridioniscus) celebesiensis sp. nov. (Sulawesi Tengah) is a characteristic species with an apomorphic lack of claw spurs and intricate dorsal cuticular sculpturing. Our findings suggest that the tropical areas of the globe harbour a phylogenetically important but yet mostly uncovered portion of Earth’s tardigrade diversity.
The Effect of Sedentary Behaviour on Cardiorespiratory Fitness: A Systematic Review and Meta-Analysis
Authors: Prince Stephanie A. , Dempsey Paddy C. , Reed Jennifer L. , Rubin Lukas , Saunders Travis J. , Ta Josephine , Tomkinson Grant R. , Merucci Katherine , Lang Justin J. 
Published: January 2024

Abstract
Background
Cardiorespiratory fitness (CRF) is an important indicator of current and future health. While the impact of habitual physical activity on CRF is well established, the role of sedentary behaviour (SB) remains less understood.

Objective
We aimed to determine the effect of SB on CRF.

Methods
Searches were conducted in MEDLINE, Embase, PsycINFO, CINAHL and SPORTDiscus from inception to August 2022. Randomised controlled trials, quasi-experimental studies and cohort studies that assessed the relationship between SB and CRF were eligible. Narrative syntheses and meta-analyses summarised the evidence, and Grading of Recommendations, Assessment, Development and Evaluation (GRADE) certainty was based on evidence from randomised controlled trials.

Results
This review included 18 studies that focused on youth (four randomised controlled trials, three quasi-experimental studies, 11 cohort studies) and 24 on adult populations (15 randomised controlled trials, five quasi-experimental studies, four cohort studies). In youth and adults, evidence from randomised controlled trials suggests mixed effects of SB on CRF, but with the potential for interventions to improve CRF. Quasi-experimental and cohort studies also support similar conclusions. Certainty of evidence was very low for both age groups. A meta-analysis of adult randomised controlled trials found that interventions targeting reducing SB, or increasing physical activity and reducing SB, had a significant effect on post-peak oxygen consumption (mean difference = 3.16 mL.kg–1.min–1, 95% confidence interval: 1.76, 4.57).

Conclusions
Evidence from randomised controlled trials indicates mixed associations between SB and CRF, with the potential for SB to influence CRF, as supported by meta-analytical findings. Further well-designed trials are warranted to confirm the relationship between SB and CRF, explore the effects of SB independent from higher intensity activity, and investigate the existence of such relationships in paediatric populations.

Clinical Trial Registration
PROSPERO CRD42022356218.
The mitogen-activated protein kinase module CcSte11-CcSte7-CcPmk1 regulates pathogenicity via the transcription factor CcSte12 in Cytospora chrysosperma
Authors: Yu Lu , Yang Yuchen , Qiu Xiaolin , Xiong Dianguang , Tian Chengming 
Published: January 2024

AbstractThe pathogen Cytospora chrysosperma is the causal agent of poplar canker disease and causes considerable economic losses in China. Mitogen-activated protein kinase (MAPK) cascades play a crucial role in mediating cellular responses and Pmk1-MAPKs are indispensable for pathogenic related processes in plant pathogenic fungi. In previous studies, we demonstrated that the CcPmk1 acts as a core regulator of fungal pathogenicity by modulating a small number of master downstream targets, such as CcSte12. In this study, we identified and characterized two upstream components of CcPmk1: MAPKKK CcSte11 and MAPKK CcSte7. Deletion of CcSte11 and CcSte7, resulted in slowed growth, loss of sporulation and virulence, similar to the defects observed in the CcPmk1 deletion mutant. In addition, CcSte11, CcSte7 and CcPmk1 interact with each other, and the upstream adaptor protein CcSte50 interact with CcSte11 and CcSte7. Moreover, we explored the global regulation network of CcSte12 by transcriptional analysis between CcSte12 deletion mutants and wild-type during the simulated infection process. Two hydrolase activity GO terms (GO:0004553 and GO:0016798) and starch and sucrose metabolism (mgr00500) KEGG pathway were significantly enriched in the down-regulated genes of CcSte12 deletion mutants. In addition, a subset of glycosyl hydrolase genes and putative effector genes were significantly down-regulated in the CcSte12 deletion mutant, which might be important for fungal pathogenicity. Especially, CcSte12 bound to the CcSp84 promoter region containing the TGAAACA motif. Moreover, comparison of CcSte12-regulated genes with CcPmk1-regulated genes revealed 116 overlapping regulated genes in both CcSte12 and CcPmk1, including some virulence-associated genes. Taken together, the protein complexes CcSte11-CcSte7-CcPmk1 receive signals transmitted by upstream CcSte50 and transmit signals to downstream CcSte12, which regulates hydrolase, effectors and other genes to promote virulence. Overall, these results indicate that the CcPmk1-MAPK signaling pathway of C. chrysosperma plays a key role in the pathogenicity.
Multifunctional Nanocomposite Membranes Containing TiO2 Developed by Air-Jet-Spun Fibers for Tissue Engineering
Authors: Vázquez-Vázquez Febe Carolina , Núñez-Tapia Israel Alfonso , Guerrero-Benítez Víctor Iván , Ortiz-Magdaleno Marine , Chanes-Cuevas Osmar Alejandro , Álvarez-Pérez Marco Antonio 
Published: January 2024

AbstractThe incorporation of inorganic nanoparticles (NP) into nanofibrous polymeric membranes (NPM) is an attractive approach to developing multifunctional nanocomposites. The purpose of this study was to incorporate titanium dioxide (TiO2) NP into NPM to enhance the overall properties. Polycaprolactone (PCL) membranes with TiO2-NP (0.2 wt%) was fabricated by means of the Air-Jet Spinning (AJS) technique. The physicochemical characterization of the PCL-TiO2-NPM was performed by X-ray powder diffraction (XRD), the morphology was observed under scanning electron microscopy (SEM), and tensile strength and Young’s moduli were evaluated using an INSTRON Universal Testing Machine. In-vitro biocompatibility was evaluated in terms of cell adhesion and cell proliferation using human fetal osteoblasts. The results obtained demonstrated that TiO2-NP added to the PCL-NPM increases the values of tensile strength and Young’s moduli (p < 0.008 and p < 0.043) with respect to PCL-NPM without TiO2-NP. XRD exhibited the characteristic peaks of PCL at 21.3° and 23.7° and of TiO2 at 36°. The SEM micrographs revealed a random distribution with interconnected micropores. Cell adhesion and proliferation increased according to the time of the culture; only after the first period of cell culture was there a significant difference in cell proliferation (p < 0.05). The possible potential application of this PCL-PNM by Air-Jet Spinning (AJS) in tissue engineering could favor bone regeneration due that the addition of TiO2-NP.
Temporal trends of carbonated soft-drink consumption among adolescents aged 12–15 years from eighteen countries in Africa, Asia and the Americas
Authors: Smith Lee , López Sánchez Guillermo Felipe , Tully Mark A , Rahmati Masoud , Oh Hans , Kostev Karel , Butler Laurie T. , Barnett Yvonne , Keyes Helen , Shin Jae Il , Koyanagi Ai 
Published: January 2024

AbstractCarbonated soft-drink consumption is detrimental to multiple facets of adolescent health. However, little is known about temporal trends in carbonated soft-drink consumption among adolescents, particularly in non-Western countries. Therefore, we aimed to examine this trend in representative samples of school-going adolescents from eighteen countries in Africa, Asia and the Americas. Cross-sectional data from the Global School-based Student Health Survey 2009–2017 were analysed. Carbonated soft-drink consumption referred to drinking carbonated soft-drinks at least once per day in the past 30 d. The prevalence of carbonated soft-drink consumption was calculated for each survey, and crude linear trends were assessed by linear regression models. Data on 74 055 students aged 12–15 years were analysed (mean age 13·9 (sd 1·0) years; 49·2 % boys). The overall mean prevalence of carbonated soft-drink consumption was 42·1 %. Of the eighteen countries included in the study, significant decreasing, increasing and stable trends of carbonated soft-drink consumption were observed in seven, two and nine countries, respectively. The most drastic decrease was observed in Kuwait between 2011 (74·4 %) and 2015 (51·7 %). Even in countries with significant decreasing trends, the decrease was rather modest, while some countries with stable trends had very high prevalence across time (e.g. Suriname 80·5 % in 2009 and 79·4 % in 2016). The prevalence of carbonated soft-drink consumption was high in all countries included in the present analysis, despite decreasing trends being observed in some. Public health initiatives to reduce the consumption of carbonated soft-drink consumption among adolescents are urgently required.
Inflammatory recruitment of healthy hematopoietic stem and progenitor cells in the acute myeloid leukemia niche
Authors: Chen Ding-Wen , Fan Jian-Meng , Schrey Julie M. , Mitchell Dana V. , Jung Seul K. , Hurwitz Stephanie N. , Perez Empar B. , Muraro Mauro J. , Carroll Martin , Taylor Deanne M. , Kurre Peter 
Published: January 2024

AbstractInflammation in the bone marrow (BM) microenvironment is a constitutive component of leukemogenesis in acute myeloid leukemia (AML). Current evidence suggests that both leukemic blasts and stroma secrete proinflammatory factors that actively suppress the function of healthy hematopoietic stem and progenitor cells (HSPCs). HSPCs are also cellular components of the innate immune system, and we reasoned that they may actively propagate the inflammation in the leukemic niche. In two separate congenic models of AML we confirm by evaluation of the BM plasma secretome and HSPC-selective single-cell RNA sequencing (scRNA-Seq) that multipotent progenitors and long-lived stem cells adopt inflammatory gene expression programs, even at low leukemic infiltration of the BM. In particular, we observe interferon gamma (IFN-γ) pathway activation, along with secretion of its chemokine target, CXCL10. We show that AML-derived nanometer-sized extracellular vesicles (EVAML) are sufficient to trigger this inflammatory HSPC response, both in vitro and in vivo. Altogether, our studies indicate that HSPCs are an unrecognized component of the inflammatory adaptation of the BM by leukemic cells. The pro-inflammatory conversion and long-lived presence of HSPCs in the BM along with their regenerative re-expansion during remission may impact clonal selection and disease evolution.
Age of onset of obsessive-compulsive disorder differentially affects white matter microstructure
Authors: Vriend Chris , de Joode Niels T. , Pouwels Petra J. W. , Liu Feng , Otaduy Maria C. G. , Pastorello Bruno , Robertson Frances C. , Ipser Jonathan , Lee Seonjoo , Hezel Dianne M. , van Meter Page E. , Batistuzzo Marcelo C. , Hoexter Marcelo Q. , Sheshachala Karthik , Narayanaswamy Janardhanan C. , Venkatasubramanian Ganesan , Lochner Christine , Miguel Euripedes C. , Reddy Y. C. Janardhan , Shavitt Roseli G. , Stein Dan J. , Wall Melanie , Simpson Helen Blair , van den Heuvel Odile A. 
Published: January 2024

AbstractPrevious diffusion MRI studies have reported mixed findings on white matter microstructure alterations in obsessive-compulsive disorder (OCD), likely due to variation in demographic and clinical characteristics, scanning methods, and underpowered samples. The OCD global study was created across five international sites to overcome these challenges by harmonizing data collection to identify consistent brain signatures of OCD that are reproducible and generalizable. Single-shell diffusion measures (e.g., fractional anisotropy), multi-shell Neurite Orientation Dispersion and Density Imaging (NODDI) and fixel-based measures, were extracted from skeletonized white matter tracts in 260 medication-free adults with OCD and 252 healthy controls. We additionally performed structural connectome analysis. We compared cases with controls and cases with early (<18) versus late (18+) OCD onset using mixed-model and Bayesian multilevel analysis. Compared with healthy controls, adult OCD individuals showed higher fiber density in the sagittal stratum (B[SE] = 0.10[0.05], P = 0.04) and credible evidence for higher fiber density in several other tracts. When comparing early (n = 145) and late-onset (n = 114) cases, converging evidence showed lower integrity of the posterior thalamic radiation —particularly radial diffusivity (B[SE] = 0.28[0.12], P = 0.03)—and lower global efficiency of the structural connectome (B[SE] = 15.3[6.6], P = 0.03) in late-onset cases. Post-hoc analyses indicated divergent direction of effects of the two OCD groups compared to healthy controls. Age of OCD onset differentially affects the integrity of thalamo-parietal/occipital tracts and the efficiency of the structural brain network. These results lend further support for the role of the thalamus and its afferent fibers and visual attentional processes in the pathophysiology of OCD.
Dopamine neuron degeneration in the Ventral Tegmental Area causes hippocampal hyperexcitability in experimental Alzheimer’s Disease
Authors: Spoleti Elena , La Barbera Livia , Cauzzi Emma , De Paolis Maria Luisa , Saba Luana , Marino Ramona , Sciamanna Giuseppe , Di Lazzaro Vincenzo , Keller Flavio , Nobili Annalisa , Krashia Paraskevi , D’Amelio Marcello 
Published: January 2024

AbstractEarly and progressive dysfunctions of the dopaminergic system from the Ventral Tegmental Area (VTA) have been described in Alzheimer’s Disease (AD). During the long pre-symptomatic phase, alterations in the function of Parvalbumin interneurons (PV-INs) are also observed, resulting in cortical hyperexcitability represented by subclinical epilepsy and aberrant gamma-oscillations. However, it is unknown whether the dopaminergic deficits contribute to brain hyperexcitability in AD. Here, using the Tg2576 mouse model of AD, we prove that reduced hippocampal dopaminergic innervation, due to VTA dopamine neuron degeneration, impairs PV-IN firing and gamma-waves, weakens the inhibition of pyramidal neurons and induces hippocampal hyperexcitability via lower D2-receptor-mediated activation of the CREB-pathway. These alterations coincide with reduced PV-IN numbers and Perineuronal Net density. Importantly, L-DOPA and the selective D2-receptor agonist quinpirole rescue p-CREB levels and improve the PV-IN-mediated inhibition, thus reducing hyperexcitability. Moreover, similarly to quinpirole, sumanirole – another D2-receptor agonist and a known anticonvulsant – not only increases p-CREB levels in PV-INs but also restores gamma-oscillations in Tg2576 mice. Conversely, blocking the dopaminergic transmission with sulpiride (a D2-like receptor antagonist) in WT mice reduces p-CREB levels in PV-INs, mimicking what occurs in Tg2576. Overall, these findings support the hypothesis that the VTA dopaminergic system integrity plays a key role in hippocampal PV-IN function and survival, disclosing a relevant contribution of the reduced dopaminergic tone to aberrant gamma-waves, hippocampal hyperexcitability and epileptiform activity in early AD.
GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4
Authors: Chen Yuting , Feng Yuanyuan , Lin Yanling , Zhou Xiaohan , Wang Lingzhi , Zhou Yingtong , Lin Kefan , Cai Longmei 
Published: January 2024

Abstract
Background
Radiotherapy is a critical treatment modality for nasopharyngeal carcinoma (NPC). However, the mechanisms underlying radiation resistance and tumour recurrence in NPC remain incompletely understood.

Methods
Oxidised lipids were assessed through targeted metabolomics. Ferroptosis levels were evaluated using cell viability, clonogenic survival, lipid peroxidation, and transmission electron microscopy. We investigated the biological functions of glutathione S-transferase mu 3 (GSTM3) in cell lines and xenograft tumours. Co-immunoprecipitation, mass spectrometry, and immunofluorescence were conducted to explore the molecular mechanisms involving GSTM3. Immunohistochemistry was performed to investigate the clinical characteristics of GSTM3.

Results
Ionising radiation (IR) promoted lipid peroxidation and induced ferroptosis in NPC cells. GSTM3 was upregulated following IR exposure and correlated with IR-induced ferroptosis, enhancing NPC radiosensitivity in vitro and in vivo. Mechanistically, GSTM3 stabilised ubiquitin-specific peptidase 14 (USP14), thereby inhibiting the ubiquitination and subsequent degradation of fatty acid synthase (FASN). Additionally, GSTM3 interacted with glutathione peroxidase 4 (GPX4) and suppressed GPX4 expression. Combining IR treatment with ferroptosis inducers synergistically improved NPC radiosensitivity and suppressed tumour growth. Notably, a decrease in GSTM3 abundance predicted tumour relapse and poor prognosis.

Conclusions
Our findings elucidate the pivotal role of GSTM3 in IR-induced ferroptosis, offering strategies for the treatment of radiation-resistant or recurrent NPC.
Phosphodiesterase type 5 inhibitors do not prevent curvature progression but shorten pain duration in the active phase of Peyronie’s Disease: A retrospective cohort study
Authors: Durukan Emil , Kraglund Rune , Rashid Shad Azad , Thorkilgaard Tine , Jensen Christian Fuglesang Skjødt , Fode Mikkel 
Published: January 2024

AbstractTreatment with Phosphodiesterase Type 5 inhibitors (PDE5is) has shown promise in managing Peyronie’s disease (PD) during its active phase. In a retrospective cohort study of 133 PD patients, we compared daily PDE5i treatment (sildenafil 25 mg or tadalafil 5 mg) in Group 1 (n = 101) to no treatment in Group 2 (n = 32). The mean age ± SD was 58.5 ± 10, (range: 29-77) years in Group 1 and 59 ± 13.7 years (range: 23-80) in Group 2 (p = 0.5). Mean symptom onset-to-visit time was 10.6 ± 7.2 months (range: 1-37) in Group 1 and 11 ± 6.3 months (range 3-27) in Group 2 (p = 0.5). Mean penile curvature change was +0.87° (95% CI: -1.8, 3.5) in Group 1 and +5.72° (95% CI: 1.4, 10) in Group 2 (p = 0.07) between first and last observations. Group 1 experienced shorter mean pain duration (9.1 ± 4.7 months, range: 2.5-24) than Group 2 (12.2 ± 6.5 months, range: 5-28) (p = 0.04). When controlling for baseline curvature and symptom onset-to-visit time, there were no differences between groups (-4.7, 95% CI: -10, 0.6) (p = 0.08). In conclusion, continuous PDE5i treatment did not affect PD curvature progression but showed a promising effect on pain.
Creativity development of tourism villages in Bandung Regency, Indonesia: co-creating sustainability and urban resilience
Authors: Buchari Rd Ahmad , Abdillah Abdillah , Widianingsih Ida , Nurasa Heru 
Published: January 2024

AbstractThis study aims to explore the interactions between the government, the tourism industry, universities, media, society, and the environment in the management and utilization of tourist villages in Bandung Regency, Indonesia. The research employed a qualitative-explorative method with a case study approach. Research analysis was assisted by the Nvivo 12 Plus qualitative analysis tool. The result's findings show that tourist villages require coordinated efforts from the government, tourism sector, universities, media, local communities, and the environment because in our opinion the environment/nature cannot only be viewed as an object but must rather be aligned with other important sectors in development programs. This connection may at the very least provide a means of enhancing the management of tourism villages and achieving sustainability and resilience. The contribution of this research provides insight into the process of developing creative tourism villages in realizing sustainability and resilience through tourism villages that pay attention to economic, social, infrastructure, and environmental dimensions.