bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multinucleate cell angiohistiocytoma: a fibrohistiocytic proliferation with increased mast cell numbers and vascular hyperplasia
Authors:   Lluís Puig, Fernández‐Figueras María‐Teresa , Bielsa Isabel , Lloveras Betlem ,   Agustín Alomar
Published: March 2003

Background: Multinucleate cell angiohistiocytoma (MCAH) is an uncommon lesion clinically characterized by multiple papules usually located on the face and acral regions of elderly women. Histopathologically, MCAH is characterized by dermal vascular hyperplasia associated with increased number of factor XIIIa‐positive fibrohistiocytic cells and multinucleate cells with scalloped borders. Methods: We report the clinical, histopathological and immunohistochemical features of three cases of MCAH, with ulstrastructural study in one of them. The patients were a woman and two men of 56, 40 and 70 years of age, respectively. They all had multiple dull‐red papules, which had appeared over several years and were located on the face, the trunk and the dorsa of the hands, respectively. Results: The reticular dermis presented a fibrohistiocytic proliferation of factor XIIIa‐positive cells, with abundant bizarre multinucleate cells and vascular hyperplasia. Increased mast cell numbers were seen in all cases, often in apposition to multinucleate cells. Conclusion: Histopathological differential diagnosis of MCAH includes mainly angiofibromas and dermatofibromas, even though vascular hyperplasia can be prominent and has led to many authors to classify MCAH among vascular tumors. Bizarre multinucleate cells can be found in reactive, neoplastic and inflammatory lesions in many sites of the body, and mast cells can play a role in their morphogenesis.
Reduction of expression of tuberin, the tuberous‐sclerosis‐complex‐gene‐2 product in tuberous sclerosis complex associated connective tissue nevi and sporadic squamous and basal cell carcinomas
Authors: Wienecke Ralf , Klemm Eckart , Karparti Sarolta , Swanson Neil A. , Green Andrew J. , DeClue Jeffrey E. 
Published: March 2003

Background: Patients affected with tuberous sclerosis complex (TSC) are prone to the development of multiple benign tumors of the skin and other organs. Tuberin, the protein product of the tuberous‐sclerosis‐complex‐2 tumor suppressor gene (TSC2) has been shown to inhibit cell proliferation. In TSC associated kidney tumors and sporadic brain tumors the loss/reduction of tuberin has been shown. Methods: Specimens of nine squamous cell carcinomas (SCC) and five basal cell carcinomas (BCC) from patients without TSC and six biopsies of connective tissue nevi (CTN) of patients with TSC were obtained. Specimens were analyzed by immunoblotting for the expression of tuberin. Results: Absent or reduced levels of tuberin were detected in the dermal parts of three of six shagreen patches, two of five BCC, and four of nine SCC. Conclusions: In tumors/hamartomas of patients with TSC the complete loss of TSC2 and tuberin is a mechanism which could be shown for CTN, thereby excluding the possibility of haploinsufficiency of TSC2. In a substantial number of cutaneous BCC and SCC the loss or downregulation of tuberin seems to be epigenetic, as alterations of TSC2 are not known in these tumors. The absence or reduction of tuberin might contribute to their proliferation.
Angiofibroblastoma of the skin: a report of seven cases in support of a distinctive entity
Authors: Diaz‐Cascajo C. , Schaefer D. , Borghi S. 
Published: March 2003

Background:  Angiofibroblastoma of the skin is a recently described benign tumor with fibroblastic differentiation. The tumor is composed of stellate and spindle fibroblasts embedded in a fibromyxoid and fibrous matrix that contains numerous capillary‐sized blood vessels.Methods:  All cases of fibromyxoid tumors of the skin from the files of two dermatopathologic centers and from consultation cases, together with clinical data and histopathologic specimens, were reviewed. Additionally, immunohistochemical studies were performed in all cases.Results:  Seven cases of angiofibroblastoma of the skin were found, including two cases previously reported. All cases showed similar histopathologic findings. The morphologic versatility of fibroblasts, however, explains some of the microscopic variations that were observed in three cases of this series. Immunohistochemical studies support the fibroblastic nature of the neoplasms.Conclusions:  Our series provides data suggesting that angiofibroblastoma of the skin represents a distinctive entity, which should be considered in the differential diagnosis of other fibromyxoid tumors of the skin.
Microtubule associated protein (MAP‐2) expression defines the companion layer of the anagen hair follicle and an analogous zone in the nail unit
Authors: Hallman James R. , Fang Dong , Setaluri Vijayasaradhi , White Wain L. 
Published: March 2003

Background:  Microtubule associated protein 2 (MAP‐2) is one of a group of polypeptides that are an integral component of the microtubular cytoskeletal structure of the central and peripheral nervous system. During the course of another investigation that utilized immunohistochemistry, MAP‐2 expression was observed in the hair follicle, almost exclusively in the innermost layer of the outer root sheath of the anagen follicle. This innermost layer, the so‐called companion layer, has unique properties that clearly distinguish it from the rest of the outer root sheath. Among these are diminished glycogenation and an intimate association with the Henle's layer, which it directly apposes. Numerous intercellular connections exist between the companion layer and Henle's layer and, in fact, the companion layer accompanies Henle's layer during its vertical ascent in the follicle. Circumferentially oriented keratin filaments have also been demonstrated between the companion layer and Henle's layer, apparently providing structural support to the inner root sheath. Experimentally, disturbances in the keratin filaments of the companion layer in animals ultimately results in destruction of the hair follicle and an alopecia.Methods:  Immunohistochemical studies for MAP‐2 were performed on 25 additional paraffin‐embedded scalp specimens using standard techniques. Because of the parallels between the follicle and nail, MAP‐2 expression in the nail unit was also investigated in three specimens.Results:  The presence of MAP‐2 in the companion layer was confirmed in all cases. Intense MAP‐2 expression in the companion layer begins at the B‐fringe (the start of the keratogenous zone with cornification of the Henle's layer of the inner sheath) and extends to the level of the isthmus where the inner root sheath exfoliates. MAP‐2 is also expressed in the upper layers of the nail matrix.Conclusion: The expression of MAP‐2 almost exclusively in the companion layer is probably related to the unique cytoskeletal structure of this microanatomic layer. Since experimental evidence has shown that the cell cytoskeleton is important to the integrity of the hair follicle, it is probable that MAP‐2 expression is also important and disturbances in its expression could play a role in the pathogenesis of some alopecias. MAP‐2 may play a similar role in the nail matrix.
Parvovirus B19 associated adult Henoch Schönlein purpura
Authors: Cioc Adina M. , Sedmak Daniel D. , Nuovo Gerard J. , Dawood Magdy R. , Smart Gerry , Magro Cynthia M. 
Published: March 2003

Background:  Parvovirus B19 has recently been implicated in various vasculitic syndromes including Henoch Schönlein purpura (HSP), Wegener's granulomatosis and microscopic polyarteritis. The association was established through serology, the identification of DNA in the peripheral blood and affected tissues and more recently by RNA localization to cutaneous capillary endothelium. However, direct localization of the viral DNA to the glomerular and cutaneous endothelium in HSP in correlation with the histopathologic findings has not been demonstrated.Methods:  Skin and kidney biopsy tissues were processed for hematoxylin and eosin, immunofluorescent, polymerase chain reaction (PCR) and reverse transcriptase in situ PCR studies.Case presentation:  A 64‐year‐old‐female presented with palpable purpura and nephrotic range proteinuria. Kidney and skin biopsies showed IgA‐associated mesangioproliferative glomerulonephritis and IgA‐associated leukocytoclastic vasculitis, respectively. A diagnosis of HSP was rendered. Her clinical course was refractory to prednisone. Parvovirus B19 DNA and tumor necrosis factor α DNA were identified in the dermal and glomerular capillary endothelial cells and surrounding dermal inflammatory cells.Conclusion:  This is the first documentation of B19 localization to dermal and glomerular capillary endothelium in HSP. It is important to recognize parvovirus B19‐associated adult HSP cases, as the treatment of choice is intravenous gamma globulin in concert with anti‐TNFα therapy. In contrast immunosuppressive therapy may lead to a persistent and/or worsening disease course.
Alcohol, Drug Abuse, and Mental Health Care for Uninsured and Insured Adults
Authors: Wells Kenneth B. , Sherbourne Cathy Donald , Sturm Roland , Young Alexander S. , Audrey Burnam M. 
Published: March 2003

ObjectiveTo compare adults with different insurance coverage in care for alcohol, drug abuse, and mental health (ADM) problems.Data Sources/Study Setting.From a national telephone survey of 9,585 respondents.Design.Follow‐up of adult participants in the Community Tracking Study.Data Collection.Self‐report survey of insurance plan (Medicare, Medicaid, unmanaged, fully, or partially managed private, or uninsured), ADM need, use of ADM services and treatments, and satisfaction with care in the last 12 months.Principal Methods.Logistic and linear regressions were used to compare persons by insurance type in ADM use.Principal FindingsThe likelihood of ADM care was highest under Medicaid and lowest for the uninsured and those under Medicare. Perceived unmet need was highest for the uninsured and lowest under Medicare. Persons in fully rather than partially managed private plans tend to be more likely to have ADM care and ADM treatments given need. Satisfaction with care was high in public plans and low for the uninsured.Conclusions.The uninsured have the most problems with access to and quality of ADM care, relative to the somewhat comparable Medicaid population. Persons in fully managed plans had better rather than worse access and quality compared to partially managed plans, but findings are exploratory. Despite low ADM use, those with Medicare tend to be satisfied. Across plans, unmet need for ADM care was high, suggesting changes are needed in policy and practice.
p63 expression in normal human epidermis and epidermal appendages and their tumors
Authors: Tsujita‐Kyutoku Miki , Kiuchi Katsuji , Danbara Naoyuki , Yuri Takashi , Senzaki Hideto , Tsubura Airo 
Published: March 2003

Background:  p63, a member of the p53 gene family, is expressed in basal cells of several different organs.Methods:  The immunoreactivity of p63 was examined in normal human epidermis and epidermal appendages and their tumors, and compared with proliferative activity as evaluated by Ki‐67.Results:  In normal skin, p63 expression was seen in basal/suprabasal cells of the epidermis, outer root sheath and hair matrix cells of the hair follicle, seboblast situated in the outermost layer of sebaceous glands, and outer layer cells of the ductal portion and myoepithelial cells of the secretory portion of the sweat glands. p63 expression was confined to the cells forming a continuous basal rim along the normal epithelial structure. In tumors, p63 expression resembled that in normal tissue in that tumor components originating from p63‐positive cells were constantly positive for p63. In normal and tumor tissues, not all p63‐positive cells were positive for Ki‐67.Conclusions:  p63 expression may be a marker of basal/progenitor cells in tumors of epidermis and epidermal appendages, and may be a diagnostic marker of these tumors.
Immunomodulatory effects of moxifloxacin in comparison to ciprofloxacin and G‐CSF in a murine model of cyclophosphamide‐induced leukopenia†
Authors: Shalit Itamar , Kletter Yehudith , Halperin Drora , Waldman Dalia , Vasserman Elad , Nagler Arnon , Fabian Ina 
Published: March 2003

Abstract: We analyzed the effect of the two quinolones moxifloxacin and ciprofloxacin on the repopulation of hematopoietic organs and on the production of cytokines by various organs of cyclophosphamide (CP)‐induced leukopenic mice. The effect was compared to that of G‐CSF. Cyclophosphamide injection induced a severe leukopenia, with nadir at day 4 post‐injection. All the quinolone and G‐CSF‐treated animals showed WBC>500/μL at the nadir, compared to 50% of saline‐treated mice. Cyclophosphamide induced a marked decrease in the number of myeloid progenitors (CFU‐C) in bone marrow (BM) and spleen. Quinolone or G‐CSF treatment resulted in a 1.4–4.3‐fold increase in CFU‐C numbers in the BM; no enhancement was observed in the spleen. Treatment with CP resulted in enhanced colony‐stimulating activity (CSA) in bone shaft and spleen and decreased activity in bladder and lung. Treatment of CP‐injected mice with quinolones significantly enhanced CSA in the bone shaft, spleen, lung and bladder on different days.In normal mice the highest levels of GM‐CSF and IL‐6 were observed in lung‐conditioned medium (compared to bone shaft, spleen and bladder). Injection of CP resulted in a 22.5‐ and 93‐fold decrease in GM‐CSF and IL‐6 levels, respectively, in lung‐conditioned medium, while treatment with quinolones resulted in 2–4‐fold increase in GM‐CSF with no effect on IL‐6 production. G‐CSF treatment had no enhancing effect on GM‐CSF nor on IL‐6 production. We conclude that moxifloxacin and ciprofloxacin administered to CP‐injected mice revert some of the immune suppressive effects of cyclophosphamide.
Anthracyclines potentiate activity against murine leukemias L1210 and P388 in vivo and in vitro
Authors: Szmigielska‐Kaplon Anna , Ciesielska Ewa , Szmigiero Leszek , Robak Tadeusz 
Published: March 2003

Abstract:  The interactions of 2‐chlorodeoxyadenosine (2‐CdA, cladribine) and three anthracyclines: doxorubicin (DOX), idarubicin (IDA) and mitoxantrone (MIT) were evaluated on murine leukemias P388 and L1210. Prolongation of survival time of animals receiving drugs in combination compared to mice treated with drugs in monotherapy was tested. We have also evaluated interactions of the cytostatics on murine leukemias in vitro by measuring their inhibitory effects on P388 and L1210 cell proliferation. We have observed a synergistic effect of MIT and IDA in combination with 2‐CdA on P388 leukemia resulting in an increase of life span (ILS)=226% in case of MIT+2‐CdA and ILS=126% in the case of IDA+2‐CdA, whereas 2‐CdA used as a sole drug resulted in an ILS=47%. The survival time of animals inoculated with P388 leukemic cells and treated with DOX+ 2‐CdA was similar to ILS gained by DOX monotherapy (178% and 200% respectively). The mice bearing L1210 leukemia receiving combined chemotherapy lived significantly longer than the animals on single agent regimens. The animals treated with schedule 2‐CdA+MIT lived significantly longer (P=0.004) as compared to the groups receiving drugs in monotherapy (ILS of 2‐CdA+MIT group=60%, ILS of MIT group 33%, and 2‐CdA group 33%). Finally, combination of DOX or IDA with 2‐CdA resulted in ILS =73% (2‐CdA+DOX regimen), and ILS=60% in case of 2‐CdA+IDA regimen, which is significantly higher than ILS gained on monotherapy schedules. In vitro tests revealed that all tested anthracyclines enhance the antiproliferative activity of 2‐CdA against L1210 and P388 leukemic cells (P<0.05).Our study has shown that all anthracyclines potentiate 2‐CdA antileukemic activity, both in vivo and in vitro. It failed however to point the best one to be combined with cladribine. We suggests that further clinical trials with such combinations are needed.
Disappearance of a mucosa‐associated lymphoid tissue (MALT) lymphoma of the urinary bladder after treatment for Helicobacter pylori
Authors: Van Den Bosch J. , Kropman R. F. , Blok P. , Wijermans P. W. 
Published: March 2003

Abstract: A 59‐yr old man presented with macroscopic hematuria. A non‐Hodgkin’s lymphoma of the MALT type of the urinary bladder, stage I A‐E, was diagnosed. Radiotherapy was advised, but the patient refused this kind of treatment. Because of the known relationship of a gastric MALT lymphoma and Helicobacter pylori, the patient was treated with HP eradication therapy. Afterwards the lymphoma disappeared. The patient is 3 yr later in a persistent complete remission.This is the first known case of the disappearance of a MALT lymphoma of the urinary bladder after treatment with HP eradication therapy.
Rebound hepatosplenomegaly in type 1 Gaucher disease
Authors: Tóth Judit , Erdős Melinda , Maródi László 
Published: March 2003

Abstract: A 19‐yr‐old male patient with type 1 Gaucher disease was put on regular biweekly infusions of alglucerase. After 1 yr of treatment, hepatic and splenic volumes decreased from 38 and 45 mL/kg to 31 and 34 mL/kg, respectively. In addition, hemoglobin concentration, platelet count and white cell count increased, acid phosphatase level decreased, and the patient gained weight and energy. Despite improvement, the patient refused enzyme replacement therapy (ERT) because of muscle rigidity, chest pain, trembling and anxiety, which he attributed to enzyme substitution. Two and 4.5 yr after cessation of therapy, hepatic and splenic volumes increased to 36 and 53 mL/kg and to 53 and 110 mL/kg, respectively. The patient developed non‐tractable hematuria because of compression and dislocation of the left kidney by the enlarged spleen, which necessitated splenectomy. This report suggests that cessation of ERT in Gaucher disease may result in severe and complicated rebound visceromegaly.
Flow cytometric measurement of reactive oxygen species production by normal and thalassaemic red blood cells
Authors: Amer Johnny , Goldfarb Ada , Fibach Eitan 
Published: March 2003

Abstract: Reactive oxygen species (ROS) contribute to the pathogenesis of several hereditary disorders of red blood cells (RBCs), including thalassaemia. We report here on a modified flow cytometric method for measuring ROS in normal and thalassaemic RBCs. RBCs were incubated with 0.4 mM 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA), then washed and further incubated either with or without 2 mM H2O2. Flow cytometric analysis showed that RBC fluorescence increased with time; it increased faster and reached higher intensity (by 10–30‐fold) in H2O2‐stimulated RBCs as compared to unstimulated RBCs. In both cases, the antioxidant N‐acetyl‐l‐cysteine reduced fluorescence, confirming previous reports that DCFH fluorescence is mediated by ROS. While the fluorescence of unstimulated RBCs increased with time, probably because of exposure to atmospheric oxygen, in H2O2‐stimulated RBCs fluorescence decreased after 30 min. The latter effect is most likely related to H2O2 decomposition by catalase as both sodium azide, an antimetabolite that inhibits catalase and low temperature increased the fluorescence of stimulated RBCs. Washing had a similar effect, suggesting that maintenance of the oxidised DCF requires a constant supply of ROS. We next studied RBCs of β‐thalassaemic patients. The results demonstrated a significantly higher ROS generation by stimulated and unstimulated thalassaemic RBCs compared to their normal counterparts. These results suggest that flow cytometry can be useful for measuring the ROS status of RBCs in various diseases and for studying chemical agents as antioxidants.
Neuromediators and pigmentation
Authors: Abdel‐Malek Zalfa 
Published: March 2003

FONDATION RENE TOURAINE POUR LA RECHERCHE EN DERMATOLOGIE SCIENTIFIC MEETING 2001 Thursday 15th November 2001, Paris, France Ministère de la Recherche et de la Technologie Amphithéâtre Poincaré, 1, rue Descartes, F‐75005 Paris *** NEUROPHYSIOLOGY OF THE SKIN 9h30–10h30 E. DUPIN Embryogenesis of neuro‐epithelial interactions10h30–11h15 D. METZE Functional anatomy of the skin nervous system11h15–12h00 T. LUGER Neuroimmunology12h00     Winners of fellowships12h30     Cocktail and Lunch14h30–15h00 H. O. HANDWERKER Neuromediators, pruritus and pain15h00–15h30 J. ANSEL Neuromediators and inflammation15h30–16h00 Z. ABDEL‐MALEK Neuromediators and pigmentation Break 16h30–17h00 R. PAUS Stress and skin17h00–17h30 N. W. BUNNETT Pharmacological modulation of skin neuromediators (PAR II)La Fondation René Touraine est une Fondation reconnue d’utilité publique.Les Membres Fondateurs sont: les Laboratoires BAILLEUL, BERGADERM, BOOTS‐LUTSIA, CHANEL, PIERRE FABRE, FONDATION LEO, GALDERMA, LABORATOIRES SÉROBIOLOGIQUES, LA ROCHE POSAY, L’OREAL, L.V.M.H., ROCHE, SCHERING PLOUGH. Inscription : Fondation René Touraine, Hôpital Saint‐Louis, Pavillon Bazin, 1, Avenue Claude Vellefaux – 75475 – Paris – France. Tél : ++33 1 53 72 20 60 – Fax : ++33 1 53 72 20 61 – e‐mail: fond.r.touraine@chu-stlouis.fr
Simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles
Authors: Magerl Markus , Kauser Söbia , Paus Ralf , Tobin Desmond J. 
Published: March 2003

Abstract: Study of the involvement of the hair follicle papilla in hair growth regulation was greatly facilitated by the isolation and cultivation of this tiny cluster of fibroblast‐like cells in the rat vibrissae and in the human hair follicle. While isolation of the hair follicle papilla from the former is relatively straightforward, the current method to isolate the much smaller human hair follicle requires significant skill. Thus, the routine initiation of primary cultures of human scalp hair follicle papilla cells requires significant training, time, and commitment.In an attempt to simplify hair follicle papilla cell culture methodology for new laboratory personnel, we have made significant refinements to the current method. Our method requires only two simple manipulations to isolate hair follicle papilla from intact isolated hair follicles. This very rapid and easy method isolates clean and intact hair follicle papillae. Together with their attachment via scratching to the growth surface, the isolation and cultivation of this important hair follicle component can now be achieved easily by the laboratory newcomer. The method relies for its simplicity on the removal of the hair follicle papilla from the outside of the intact hair follicle rather than via internal manipulations from within the hair follicle.