bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Biofabrication Platform for Collagen‐Based Peripheral Nerve Grafts with Structural and Chemical Guidance
Authors: Wang Heran , Hao Yiming , Guo Kai , Liu Lianqing , Xia Bing , Gao Xue , Zheng Xiongfei , Huang Jinghui 
Published: November 2023

AbstractOwing to its crucial role in the human body, collagen has immense potential as a material for the biofabrication of tissues and organs. However, highly refined fabrication using collagen remains difficult, primarily because of its notably soft properties. A quantitative biofabrication platform to construct collagen‐based peripheral nerve grafts, incorporating bionic structural and chemical guidance cues, is introduced. A viscoelastic model for collagen, which facilitates simulating material relaxation and fabricating collagen‐based neural grafts, achieving a maximum channel density similar to that of the native nerve structure of longitudinal microchannel arrays, is established. For axonal regeneration over considerable distances, a gradient printing control model and quantitative method are developed to realize the high‐precision gradient distribution of nerve growth factor required to obtain nerve grafts through one‐step bioprinting. Experiments verify that the bioprinted graft effectively guides linear axonal growth in vitro and in vivo. This study should advance biofabrication methods for a variety of human tissue‐engineering applications requiring tailored cues.
Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery
Authors: Mulero‐Russe Adriana , García Andrés J. 
Published: November 2023

AbstractHuman intestinal organoids (HIOs) derived from pluripotent stem cells or adult stem cell biopsies represent a powerful platform to study human development, drug testing, and disease modeling in vitro, and serve as a cell source for tissue regeneration and therapeutic advances in vivo. Synthetic hydrogels can be engineered to serve as analogs of the extracellular matrix to support HIO growth and differentiation. These hydrogels allow for tuning the mechanical and biochemical properties of the matrix, offering an advantage over biologically derived hydrogels such as Matrigel. Human intestinal organoids have been used for repopulating transplantable intestinal grafts and for in vivo delivery to an injured intestinal site. The use of synthetic hydrogels for in vitro culture and for in vivo delivery is expected to significantly increase the relevance of human intestinal organoids for drug screening, disease modeling, and therapeutic applications.
Zinc–Bromine Batteries: Challenges, Prospective Solutions, and Future
Authors: Mahmood Asif , Zheng Zhi , Chen Yuan 
Published: November 2023

AbstractZinc‐bromine batteries (ZBBs) have recently gained significant attention as inexpensive and safer alternatives to potentially flammable lithium‐ion batteries. Zn metal is relatively stable in aqueous electrolytes, making ZBBs safer and easier to handle. However, Zn metal anodes are still affected by several issues, including dendrite growth, Zn dissolution, and the crossover of Br species from cathodes to corrode anodes, resulting in self‐discharge and fast performance fading. Similarly, Br2 undergoes sluggish redox reactions on cathodes, which brings several issues such as poor reaction kinetics, the highly corrosive nature of Br species leading to corrosion of separators and poisoning of anodes, and the volatile nature of Br species causing increased internal pressures, etc. These issues are compounded in flowless ZBB configuration as no fresh electrolyte is available to provide extra/fresh reaction species. In this review, the factors controlling the performance of ZBBs in flow and flowless configurations are thoroughly reviewed, along with the status of ZBBs in the commercial sector. The review also summarizes various novel methodologies to mitigate these challenges and presents research areas for future studies. In summary, this review will offer a perspective on the historical evolution, recent advancements, and prospects of ZBBs.
Supramolecular Salt‐Assisted Quasi‐Solid‐State Electrolyte Promoting Dual Conductive Interface for High‐Energy‐Density Lithium Metal Batteries
Authors: Chai Simin , Zhong Yue , Wang Yijiang , He Qiong , Azizi Alireza , Chen Leyuan , Ren Xueting , Wei Weifeng , Liang Shuquan , Chang Zhi , Pan Anqiang 
Published: November 2023

AbstractIon electrokinetic regulation in gel polymer electrolytes (GPEs) is of great significance in alleviating dendrite growth and electrode corrosion of metallic lithium in lithium metal batteries (LMBs). Herein, a supramolecular LiPAAOB salt‐assisted GPEs (3D‐DIS‐1.25% GPEs) is synthesized to improve the ionic electrodynamics of LMBs. The LiPAAOB conductor salt provides sufficient Li+ ions while simultaneously facilitating LiTFSI dissociation. Theses dissociated Li+ ions can be further anchored firmly by enriched affinitive sites (─C═O, ─C─O) induced in 3D composite polymer skeleton (PALC). Due to the strong hydrogen‐bonding interactions between PALC and LiPAAOB/LiTFSI, a stable coordination structure is thereby constructed in 3D‐DIS‐1.25% GPEs, which then facilitates Li+ ions to transport rapidly along polymer chains. Consequently, the electrochemical stable window and ionic conductivity of the 3D‐DIS‐1.25% GPEs are increased to as high as 5.3 V and 1.34 mS cm−1, respectively. The Li+/e− double conducting interfaces in situ formed on Li metal anode and active particles in cathodes can effectively inhibit dendrite growth and electrode corrosion. As a result, both Li||3D‐DIS‐1.25% GPEs||Li symmetrical battery and flexible 3D‐DIS‐1.25% GPEs‐based pouch cell with high LFP loading operate stably even under folded and curly state, indicating the application possibility of using 3D‐DIS‐1.25% GPEs in constructing various flexible high‐energy‐density batteries.
A Rigid Multiple Resonance Thermally Activated Delayed Fluorescence Core Toward Stable Electroluminescence and Lasing
Authors: Tang Xun , Xie Mingchen , Lin Zesen , Mitrofanov Kirill , Tsagaantsooj Tuul , Lee Yi‐Ting , Kabe Ryota , Sandanayaka Atula S. D. , Matsushima Toshinori , Hatakeyama Takuji , Adachi Chihaya 
Published: November 2023

AbstractThe investigation of organic light‐emitting diodes (OLEDs) and organic laser devices with thermally activated delayed fluorescence (TADF) molecules is emerging due to the potential of harnessing triplets. In this work, a boron/nitrogen multiple‐resonance TADF polycyclic framework fusing carbazole units (CzBNPh) was proposed. CzBNPh exhibited a narrowband emission (<30 nm), a unity photoluminescence quantum yield, and a fast radiative rate. Consequently, CzBNPh demonstrated a low distributed feedback (DFB) lasing threshold of 0.68 μJ cm−2. Furthermore, the stimulated emission zone of CzBNPh was effectively separated from its singlet and triplet absorption, thereby minimizing the singlet‐triplet annihilation under long‐pulsed excitation ranging from 20 μs to 2.5 ms. Significantly, the enhanced rigid molecular conformation, thermal stability, and photo‐stability resulted in improved lasing and electroluminescence stability compared to that of 5,9‐diphenyl‐5,9‐diaza‐13b‐boranaphtho[3,2,1‐de]anthracene (DABNA)‐core. These findings indicate the potential of CzBN‐core as a promising framework for achieving long‐pulsed wave and electrically‐pumped lasing in the future.
The importance of parenting self‐efficacy and social support for family quality of life in children newly diagnosed with autism spectrum disorder: A one‐year follow‐up study
Authors: Dai Yushen , Chen Miaoying , Deng Tao , Huang Baoqin , Ji Yan , Feng Yongshen , Liu Shaofei , Zhang Lifeng 
Published: November 2023

AbstractImproving the quality of family life (FQoL) is one of the ultimate goals for autism spectrum disorder (ASD) intervention, and parenting self‐efficacy and social support are critical for the well‐being of families. However, longitudinal studies focusing on FQoL and its predictors for families of children with ASD are scarce. This study aims to describe the characteristics of FQoL among parents of children newly diagnosed with ASD at two waves (newly diagnosed and diagnosed after one year) and to explore the predictors of FQoL at two waves. It was conducted at a tertiary hospital in Guangzhou, China. A total of 156 parents and their children were included in Wave 1, followed up with 110 in Wave 2 after 1 year. The overall satisfaction of FQoL improved (t = −2.128, p < 0.05), while satisfaction with physical/material well‐being decreased (t = 5.972, p < 0.01). Additionally, the overall importance rating of FQoL improved but did not have statistical significance (p > 0.05). Parents with higher parenting self‐efficacy (β = 0.716, P < 0.01), and more subjective social support (β = 1.127, p < 0.001) reported higher satisfaction with FQoL, and those with better social support utilization (β = 1.066, p < 0.05) reported higher importance for FQoL. FQoL needs to be improved in the early stage of ASD diagnosis, and parental self‐efficacy and social support can serve as the intervention targets.
CRISPR‐Cas9 technology: As an efficient genome modification tool in the cancer diagnosis and treatment
Authors: Behrouzian Fard Ghazaleh , Ahmadi Mohammad Hossein , Gholamin Mehran , Amirfakhrian Razieh , Saberi Teimourian Elahe , Karimi Mohammad Ali , Hosseini Bafghi Mahdi 
Published: November 2023

AbstractCancer is the second most common cause of death globally and is a major public health concern. Managing this disease is difficult due to its multiple stages and numerous genetic and epigenetic changes. Traditional cancer diagnosis and treatment methods have limitations, making it crucial to develop new modalities to combat the increasing burden of cancer. The clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 9 (Cas9) system has transformed genetic engineering due to its simplicity, specificity, low cytotoxicity, and cost‐effectiveness. It has been proposed as an effective technology to enhance cancer diagnosis and treatment strategies. This article presents the most recent discoveries regarding the structure, mechanism, and delivery methods of the highly powerful genome editing tool, CRISPR‐Cas9. In terms of diagnosis, the article examines the role of CRISPR‐Cas9 in detecting microRNAs and DNA methylation, and discusses two popular gene detection techniques that utilize the CRISPR‐Cas system: DNA endonuclease‐targeted CRISPR trans reporter and specific high sensitivity enzymatic reporter unlocking. Regarding treatment, the article explores several genes that have been identified and modified by CRISPR‐Cas9 for effective tumorigenesis of common cancers such as breast, lung, and colorectal cancer. The present review also addresses the challenges and ethical issues associated with using CRISPR‐Cas9 as a diagnostic and therapeutic tool. Despite some limitations, CRISPR‐Cas9‐based cancer diagnosis has the potential to become the next generation of cancer diagnostic tools, and the continuous progress of CRISPR‐Cas9 can greatly aid in cancer treatment.
Eosinophil may be a predictor of immune‐related adverse events induced by different immune checkpoint inhibitor types: A retrospective multidisciplinary study
Authors: Tasaki Yoshihiko , Sugiyama Yosuke , Hamamoto Shuzo , Naiki Taku , Uemura Takehiro , Yokota Keisuke , Kawakita Daisuke , Nakamura Motoki , Ogawa Ryo , Shimura Takaya , Mimura Yoshihisa , Hotta Yuji , Odagiri Kunihiro , Ito Nanami , Iida Moeko , Kimura Yuka , Komatsu Hirokazu , Kataoka Hiromi , Takiguchi Shuji , Morita Akimichi , Iwasaki Shinichi , Okuda Katsuhiro , Niimi Akio , Yasui Takahiro , Furukawa‐Hibi Yoko 
Published: November 2023

AbstractBackgroundImmune checkpoint inhibitors (ICIs) can cause severe immune‐related adverse events (irAEs). However, biomarkers for irAEs common to different types of ICIs and cancers have not been reported. This study examined whether eosinophils can be used as a predictor of irAEs.MethodsSix hundred fourteen patients with cancer (esophageal, gastric, head and neck, lung, melanoma, renal cell, urothelial, and other cancer) received anti‐PD‐1, anti‐PD‐L1, or anti‐CTLA‐4 plus anti‐PD‐1 therapy. The patients were divided into two groups depending on whether they experienced irAEs (irAE group) or not (non‐irAE group). Eosinophils were examined before the two‐course treatment.ResultsPatients in the irAE group who received anti‐PD‐1 or anti‐CTLA‐4 plus anti‐PD‐1 therapy had higher eosinophils before the two‐course treatment than those in the non‐irAE group (p < 0.05). The eosinophils in the anti‐PD‐L1 therapy group tended to increase in the irAE group. Furthermore, eosinophils in gastric, head and neck, lung, melanoma, renal, and urothelial cancers were significantly higher in the irAE group than in the non‐irAE group (p < 0.05). The optimal cutoff value for eosinophils against irAEs was 3.0% (area under the curve = 0.668). In multivariate analyses, eosinophils of ≥3.0% were an independent factor for irAEs (odds ratio: 2.57, 95% CI: 1.79–3.67).ConclusionAn increased eosinophil before the two‐course treatment may be a predictor of irAEs in various cancers treated with different ICIs.
Synthesis and in Silico Studies of Some New 1,2,3‐Triazolyltetrazole Bearing Indazole Derivatives as Potent Antimicrobial Agents
Authors: Gujja Venkanna , Sadineni Kumaraswamy , Epuru Manohar Reddy , Rao Allaka Tejeswara , Banothu Venkanna , Gunda Shravan Kumar , Koppula Shiva Kumar 
Published: November 2023

Abstract1,2,3‐Triazole and tetrazole derivatives bearing pyrrolidines are found to exhibit notable biological activity and have become useful scaffolds in medicinal chemistry for application in lead discovery and optimization. Novel indazole bearing 1,2,3‐triazolyltetrazoles were designed as potential antimicrobial candidates. The structure of duel heterocyclics was validated by a spectroscopic technique of infrared (IR), nuclear magnetic resonance (1H and 13C NMR), and mass spectral data. Compounds 4b, 4c, 4d, and 4h were found to have a stronger antibacterial effect against Gram‐positive (S. aureus, B. subtilis, M. Luteus) and Gram‐negative (E. coli, P. aeruginosa) microorganisms with MICs ranging from 5±0.03–18±0.02 μM, respectively. Moreover, scaffolds 4a, 4h showed potent antifungal activity against A. flavus, M. gypsuem strains with MIC values of 10±0.02, 11±0.01 μM, which are similar activity that of the standard Itraconazole (MIC=8±0.02, 10±0.01 μM). The binding mode for compound 4 inside the catalytic pocket of S. aureus complexed with nicotinamide adenine dinucleotide phosphate and trimethoprim and produced a network of hydrophobic and hydrophilic interactions (3FRE). From in silico results, 4b demonstrated highly stable hydrogen binding amino acids Leu62(X) [N18…O, 2.47 Å], Arg44(X) [N17…N, 3.11 Å], Thr96(X) [N10…OG1, 3.05 Å], Gly94(X) [F7…N, 2.82 Å], and Gly43(X) [F7…N, 2.90 Å], which are plays a crucial role in ensuring efficient binding of the ligand in a crystal structure of antibacterial receptor. Furthermore, the physicochemical and ADME filtration molecular properties, estimation of toxicity, and bioactivity scores of these novel scaffolds were evaluated by using SwissADME and ADMETlab2.0 online protocols. Thus, the significant antimicrobial activity of indazole linked to duel heterocyclic compounds can be used for development of new antimicrobial agents with further modifications.
Operating performance assessment based on stacked performance‐relevant enhanced denoising auto‐encoder for industrial processes
Authors: Liu Yan , Ma Zhe , Wang Fuli , Ma Ruicheng , Chu Fei , Li Xinghua , Guan Changliang 
Published: November 2023

AbstractAs an effective way to ensure the economic benefits of enterprises, process operating performance assessment has attracted more and more attention from industry and academia in recent years. In this paper, a stacked performance‐relevant enhanced denoising autoencoder (SPEDAE) network is designed for the operating performance assessment of industrial processes. Compared to the original denoising auto‐encoder (DAE), each performance‐relevant enhanced denoising auto‐encoder (PEDAE) not only reconstructs the input features in the output layer, but also strives to reconstruct the original input data and the performance grade labels simultaneously. Then the SPEDAE is formed by stacking multiple PEDAEs layer by layer. Through this improved training strategy, SPEDAE can avoid accumulated information loss during the deep feature extraction process, improve the robustness of the network, and extract features closely related to the operating performance, thereby better completing the assessment task. The effectiveness of the proposed assessment method is validated on the case of gold cyanide leaching process. Compared with several methods, the proposed SPEDAE has the highest accuracy and reaches 99.85%, which demonstrates its superiority in operating performance assessment.
Regulating the Electron‐Deficient Component in A‐DA1D‐A Typed Small‐Molecule Acceptors for High‐Performance Organic Solar Cells†
Authors: Wei Weifei , Ma Ruijie , Chen Zhanxiang , Xu Tongle , Li Gang , Luo Zhenghui 
Published: November 2023

Comprehensive SummaryFine‐tuning of the electron‐deficient unit in A‐DA1D‐A typed small‐molecule acceptors (SMAs) plays a crucial role in developing efficient SMAs for organic solar cells (OSCs). Here, we developed a SMA based on benzo[4,5]thieno[2,3‐b]quinoxaline, designated as QW1, as well as three SMAs based on 1‐methylindoline‐2,3‐dione, identified as QW2, QW3, and QW4. Compared with QW2, QW1 displays slightly blue‐shifted absorption spectra and a lower LUMO energy level due to the stronger electron‐withdrawing capability of BTQx in contrast to MDO. On the other hand, the introduction of a bromine atom in QW3 and QW4 causes a blue shift in absorption and a reduction in the LUMO energy level compared to QW2. Density functional theory analysis reveals that QW1 exhibits the best molecular planarity, which endows QW1 with larger electron mobility and tighter molecular stacking. Consequently, PM6:QW1 device affords a better efficiency of 15.63% than those of the devices based on QW2 (14.25%), QW3 (13.21%) and QW4 (15.03%). Moreover, the QW4‐based device yields the highest open‐circuit voltage of 0.933 V, and the PM6:L8‐BO:QW4 ternary device realizes a PCE of 19.03%. Overall, our work demonstrates that regulation of electron‐deficient central units is an effective strategy to improve the photovoltaic performance of the resulting A‐DA1D‐A SMAs.
Improving Species Level‐taxonomic Assignment from 16S rRNA Sequencing Technologies
Authors: Bars‐Cortina David , Moratalla‐Navarro Ferran , García‐Serrano Ainhoa , Mach Núria , Riobó‐Mayo Lois , Vea‐Barbany Jordi , Rius‐Sansalvador Blanca , Murcia Silvia , Obón‐Santacana Mireia , Moreno Victor 
Published: November 2023

AbstractAnalysis of the bacterial community from a 16S rRNA gene sequencing technologies requires comparing the reads to a reference database. The challenging task involved in annotation relies on the currently available tools and 16S rRNA databases: SILVA, Greengenes and RDP. A successful annotation depends on the quality of the database. For instance, Greengenes and RDP have not been updated since 2013 and 2016, respectively. In addition, the nature of 16S sequencing technologies (short reads) focuses mainly on the V3‐V4 hypervariable region sequencing and hinders the species assignment, in contrast to whole shotgun metagenome sequencing.Here, we combine the results of three standard protocols for 16S rRNA amplicon annotation that utilize homology‐based methods, and we propose a new re‐annotation strategy to enlarge the percentage of amplicon sequence variants (ASV) classified up to the species level. Following the pattern (reference) method: DADA2 pipeline and SILVA v.138.1 reference database classification (Basic Protocol 1), our method maps the ASV sequences to custom nucleotide BLAST with the SILVA v.138.1 (Basic Protocol 2), and to the 16S database of Bacteria and Archaea of NCBI RefSeq Targeted Loci Project databases (Basic Protocol 3).This new re‐annotation workflow was tested in 16S rRNA amplicon data from 156 human fecal samples. The proposed new strategy achieved an increase of nearly eight times the proportion of ASV classified at the species level in contrast to the reference method for the database used in the present research. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Sample inference and taxonomic profiling through DADA2 algorithm.Basic Protocol 2: Custom BLASTN database creation and ASV taxonomical assignment.Basic Protocol 3: ASV taxonomical assignment using NCBI RefSeq Targeted Loci Project database.Basic Protocol 4: Definitive selection of lineages among the three methods.
Identifying physiological traits related with drought tolerance and water‐use efficiency in floral hemp (Cannabis sativa L.)
Authors: Morgan Wayne , Singh Jagdeep , Kesheimer Katelyn , Davis Jeanine , Sanz‐Saez Alvaro 
Published: November 2023

AbstractHemp (Cannabis sativa L.) is a reemerging crop that was once deeply rooted in many global civilizations. Due to climate change and the increasing risk of water‐deficit situations, it is important to use water judiciously. Hemp production has interested many producers; however, due to its past illegal status, there is a lack of research‐based knowledge about the physiological parameters that are affected by drought and which parameters could be responsible for drought tolerance. Therefore, our objective was to study and understand the effects of different drought intensities and timings on physiological parameters such as water‐use efficiency (WUE), photosynthesis, stomatal conductance, and fluorescence parameters and determine if cultivar variation exists as this could mean the possibility of selection for drought‐tolerant cultivars. In addition, we tested if carbon isotope discrimination can be used to predict whole plant WUE as this technique is quick and allows moderate throughput for selection. Hemp cultivars BaOx and Cherry Mom were grown in a greenhouse from July to October during 2021 and 2022 under different water stress intensities and timings. During the water stress period, WUE was determined by the gravimetric method and photosynthetic traits were measured using two portable gas analyzers (LI‐6800). Moderate water stress (30%–50% field capacity) was found to have the least effect on physiological parameters studied, while intense water stresses significantly reduced many physiological parameters such as photosynthesis, stomatal conductance (gs), and electron transport rate (ETR). It was found that the cultivar BaOx had a greater WUE than Cherry Mom. This was confirmed with carbon isotope discrimination measurements, which were demonstrated to estimate WUE accurately, and therefore, this technique could be used for germplasm screening. Drought first decreased gs, which caused the reduction in photosynthesis, although cultivar variation in these traits was not observed. Twenty days after drought, negative effects on fluoresce parameters such as ETR were observed with the cultivar Cherry Mon being more affected. This study states that hemp is negatively affected by different timings and intensities of drought; however, there is physiological variation in the response between cultivars. More research is needed to investigate the sources of genotypic variation in physiological parameters to find the sources of drought tolerance.