bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conceptualizing the Integration of Business and Private Components in Individual Information Systems
Authors: Lanzl Julia , Utz Lena , Afflerbach Patrick , Gimpel Henner 
Published: December 2023

AbstractIndividuals build their individual information system (IIS) with which they manage the boundary between different domains of life. In this age of permanent mobile accessibility, however, the boundary between the private and business domains can become blurred. While some users try to maintain a degree of separation between the two IIS sub-systems (the private and the business information system), others integrate them. Understanding such integration is essential as it affects well-being and performance in both domains. To further this understanding, we introduce a conceptualization of IIS that distinguishes between four layers of IIS: devices, digital identities, relationships, and information. To measure IIS integration, we develop a method based on the IIS components’ usage frequencies in each domain. We evaluate our conceptualization as well as our measurement method by empirically testing a theoretical model of IIS integration on multiple layers. Our data provides empirical support for the importance of relationship integration as one of the four layers. This leads us to the conclusion that even though IIS are composed of four layers, only the relationship layer is of interest regarding IIS integration. Our results are of interest and service to individuals, organizations, and IT designers who aim to manage IIS integration for individual and organizational benefits.
Phosphorus-enriched biochar from biogas residue of Eichhornia crassipes: transformation and release of phosphorus
Authors: Yu Fan , Wang Junxia , Wang Xutong , Wang Yuting , Guo Qianqian , Wang Zhi , Cui Xiaoqiang , Hu Yanjun , Yan Beibei , Chen Guanyi 
Published: December 2023

AbstractPyrolysis is an effective technology for treating and utilizing biogas residue. To explore the phosphorus (P) supply capacity of the biochar generated from biogas residue of Eichhornia Crassipes, the P speciation of E. crassipes biogas residue and biomass during pyrolysis (300–700 °C) was analyzed by combining sequential chemical extraction, 31P nuclear magnetic resonance (NMR) and P K-edge X-ray absorption near edge structure (XANES) spectroscopy. Pyrolysis treatment promoted the conversion of amorphous Ca-P phases in biogas residue and biomass into crystalline hydroxyapatite (HAP) phase, which matched the formation of stable HCl-P pools in the biochar derived from biogas residue (AEBs, 22.65–82.04%) and biomass (EBs, 13.08–33.52%) in the process of pyrolysis. Moreover, the total P contents in AEBs (19.43–28.92 mg g−1) were higher than that of EBs (3.41–5.26 mg g−1), indicating that AEBs had a great P reclamation potential. The P release kinetics from AEBs and EBs in water were evaluated via an incubation experiment for 360 h. The P release from both AEBs and EBs conformed to the pseudo-second order kinetics model (R2 > 0.93), but their P release behaviors were different. The P release of AEBs conformed to the diffusion-re-adsorption model, while that of EBs accorded with the diffusion-dissolution model. The diffusive gradients in thin-films (DGT) analysis showed that AEBs could significantly increase soil available P content as compared with EBs. Hence, the biochar produced from biogas residue of E. crassipes via pyrolysis has a good application potential as a P fertilizer.
Graphical Abstract
Application of Raman spectroscopy to the evaluation of F-actin changes in sea urchin eggs at fertilization
Authors: Mangini Maria , Limatola Nunzia , Ferrara Maria Antonietta , Coppola Giuseppe , Chun Jong Tai , De Luca Anna Chiara , Santella Luigia 
Published: December 2023

SummaryThe actin filaments on the surface of echinoderm oocytes and eggs readily undergo massive reorganization during meiotic maturation and fertilization. In sea urchin eggs, the actin cytoskeletal response to the fertilizing sperm is fast enough to accompany Ca2+ signals and to guide sperm’s entry into the egg. Although recent work using live cell imaging technology confirmed changes in the actin polymerization status in fertilized eggs, as was previously shown using light and electron microscopy, it failed to provide experimental evidence of F-actin depolymerization a few seconds after insemination, which is concurrent with the sperm-induced Ca2+ release. In the present study, we applied Raman microspectroscopy to tackle this issue by examining the spectral profiles of the egg’s subplasmalemmal regions before and after treating the eggs with actin drugs or fertilizing sperm. At both early (15 s) and late (15 min) time points after fertilization, specific peak shifts in the Raman spectra revealed change in the actin structure, and Raman imaging detected the cytoskeletal changes corresponding to the F-actin reorganization visualized with LifeAct-GFP in confocal microscopy. Our observation suggests that the application of Raman spectroscopy, which does not require microinjection of fluorescent probes and exogenous gene expression, may serve as an alternative or even advantageous method in disclosing rapid subtle changes in the subplasmalemmal actin cytoskeleton that are difficult to resolve.
Who is Interested in Participating in Participatory Budgeting?
Authors: Doherty David , Pavel Raluca G. , Jackson Madeline , Garbarski Dana 
Published: December 2023

AbstractInequalities in terms of who participates in politics yield policy outcomes that fail to reflect the interests of the broader public. Because these processes fail to engage the full citizenry in political decision-making processes, they are also markers of an anemic civic culture. Advocates of participatory budgeting (PB) – a process implemented at the subnational level in thousands of cities in the United States and beyond that invites residents to participate directly in the process of allocating public resources for local projects – argue that it can alleviate these inequalities. They argue that features of the PB process make it ripe for engaging new participants in the political process and weaving a more inclusive social fabric. We examine the correlates of interest in participating in PB using a survey of Cook County residents. We also consider the extent to which the policy priorities of those who are interested in participating diverge from those who are less interested. Although we find evidence that the process is particularly appealing to younger respondents and those who identify as Latine or Black (as opposed to White), we also find that interest is higher among those with higher socioeconomic status and those who perceive conditions in their neighborhood to already be good. Our evidence also suggests that inequalities in who is interested in participating may not radically affect policy outcomes. However, those who decline to participate cannot reap the broader social and political benefits advocates hope the PB process can foster.
A reflective display based on the electro-microfluidic assembly of particles within suppressed water-in-oil droplet array
Authors: Shen Shitao , Feng Haoqiang , Deng Yueming , Xie Shuting , Yi Zichuan , Jin Mingliang , Zhou Guofu , Mulvaney Paul , Shui Lingling 
Published: December 2023

AbstractReflective displays have stimulated considerable interest because of their friendly readability and low energy consumption. Herein, we develop a reflective display technique via an electro-microfluidic assembly of particles (eMAP) strategy whereby colored particles assemble into annular and planar structures inside a dyed water droplet to create “open” and “closed” states of a display pixel. Water-in-oil droplets are compressed within microwells to form a pixel array. The particles dispersed in droplets are driven by deformation-strengthened dielectrophoretic force to achieve fast and reversible motion and assemble into multiple structures. This eMAP based device can display designed information in three primary colors with ≥170° viewing angle, ~0.14 s switching time, and bistability with an optimized material system. This proposed technique demonstrates the basis of a high-performance and energy-saving reflective display, and the display speed and color quality could be further improved by structure and material optimization; exhibiting a potential reflective display technology.
“Puberty age gap”: new method of assessing pubertal timing and its association with mental health problems
Authors: Dehestani Niousha , Vijayakumar Nandita , Ball Gareth , Mansour L Sina , Whittle Sarah , Silk Timothy J. 
Published: December 2023

AbstractPuberty is linked to mental health problems during adolescence, and in particular, the timing of puberty is thought to be an important risk factor. This study developed a new measure of pubertal timing that was built upon multiple pubertal features and their nonlinear changes over time (i.e., with age), and investigated its association with mental health problems. Using the Adolescent Brain Cognitive Development (ABCD) cohort (N ~ 9900, aged 9–13 years), we employed three different models to assess pubertal timing. These models aimed to predict chronological age based on: (i) observed physical development, (ii) hormone levels (testosterone and dehydroepiandrosterone [DHEA]), and (iii) a combination of both physical development and hormones. To achieve this, we utilized a supervised machine learning approach, which allowed us to train the models using the available data and make age predictions based on the input pubertal features. The accuracy of these three models was evaluated, and their associations with mental health problems were examined. The new pubertal timing model performed better at capturing age variance compared to the more commonly used linear regression method. Further, the model based on physical features accounted for the most variance in mental health, such that earlier pubertal timing was associated with higher symptoms. This study demonstrates the utility of our new model of pubertal timing and suggests that, relative to hormonal measures, physical measures of pubertal maturation have a stronger association with mental health problems in early adolescence.
Long-term outcome of children with acute promyelocytic leukemia: a randomized study of oral versus intravenous arsenic by SCCLG-APL group
Authors: Huang Dan-Ping , Yang Liang-Chun , Chen Yi-Qiao , Wan Wu-Qing , Zhou Dun-Hua , Mai Hui-Rong , Li Wan-Li , Yang Li-Hua , Lan He-Kui , Chen Hui-Qin , Guo Bi-Yun , Zhen Zi-Jun , Liu Ri-Yang , Chen Guo-Hua , Feng Xiao-Qin , Liang Cong , Wang Li-Na , Li Yu , Luo Jie-Si , Fan Zhong , Luo Xue-Qun , Li Bin , Tang Yan-Lai , Zhang Xiao-Li , Huang Li-Bin 
Published: December 2023

AbstractRealgar-Indigo naturalis formula (RIF), an oral traditional Chinese medicine mainly containing Realgar (As4S4), is highly effective in treating adult acute promyelocytic leukemia (APL). However, the treatment efficacy and safety of RIF have not been verified in pediatric patients. SCCLG-APL group conducted a multicenter randomized non-inferiority trial to determine whether intravenous arsenic trioxide (ATO) can be substituted by oral RIF in treating pediatric APL. Of 176 eligible patients enrolled, 91 and 85 were randomized to ATO and RIF groups, respectively. Patients were treated with the risk-adapted protocol. Induction, consolidation, and 96-week maintenance treatment contained all-trans-retinoic acid and low-intensity chemotherapy, and either ATO or RIF. The primary endpoint was 5-year event-free survival (EFS). The secondary endpoints were adverse events and hospital days. After a median 6-year follow-up, the 5-year EFS was 97.6% in both groups. However, the RIF group had significantly shorter hospital stays and lower incidence of infection and tended to have less cardiac toxicity. All 4 relapses occurred within 1.5 years after completion of maintenance therapy. No long-term arsenic retentions were observed in either group. Substituting oral RIF for ATO maintains treatment efficacy while reducing hospitalization and adverse events in treating pediatric APL patients, which may be a future treatment strategy for APL.
Deletion of Mettl3 in mesenchymal stem cells promotes acute myeloid leukemia resistance to chemotherapy
Authors: Liao Xinai , Cai Danni , Liu Jingru , Hu Haoran , You Ruolan , Pan Zhipeng , Chen Shucheng , Xu Kaiming , Dai Wei , Zhang Shuxia , Lin Xinjian , Huang Huifang 
Published: December 2023

AbstractAcute myeloid leukemia (AML) cell survival and chemoresistance are influenced by the existence of bone marrow mesenchymal stem cells (BMMSCs); however, the pathways by which BMMSCs contribute to these processes remain unclear. We earlier revealed that methyltransferase-like 3 (METTL3) expression is significantly reduced in AML BMMSCs and that METTL3 mediates BMMSC adipogenesis to promote chemoresistance in human AML cell lines in vitro. In this investigation, we evaluated the METTL3 function in vivo. Mice exhibiting a conditional removal of Mettl3 in BMMSCs were developed by mating Prrx1-CreERT2;Mettl3fl/+ mice with Mettl3fl/fl mice using the CRISPR-Cas9 system. The Mettl3 deletion increased bone marrow adiposity, enhanced disease progression in the transplantation-induced MLL-AF9 AML mouse model, and chemoresistance to cytarabine. The removal of Mettl3 in BMMSCs resulted in a significant increase in BMMSC adipogenesis. This effect was attributed to the downregulation of AKT1 expression, an AKT serine/threonine kinase 1, in an m6A-dependent manner. The development of chemoresistance in AML is linked to the promoted adipogenesis of BMMSCs. We conclude that METTL3 expression in BMMSCs has a critical function in limiting AML progression and chemoresistance, providing a basis for the progression of therapeutic approaches for AML.
Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase
Authors: Lin Folan , Zhang Ruxin , Shao Weihan , Lei Cong , Ma Mingxi , Zhang Ying , Wen Zengqi , Li Wanqiu 
Published: December 2023

AbstractHistone lysine methyltransferase SUV420H1, which is responsible for site-specific di-/tri-methylation of histone H4 lysine 20 (H4K20), has crucial roles in DNA-templated processes, including DNA replication, DNA damage repair, and chromatin compaction. Its mutations frequently occur in human cancers. Nucleosomes containing the histone variant H2A.Z enhance the catalytic activities of SUV420H1 on H4K20 di-methylation deposition, regulating early replication origins. However, the molecular mechanism by which SUV420H1 specifically recognizes and deposits H4K20 methyl marks on nucleosomes remains poorly understood. Here we report the cryo-electron microscopy structures of SUV420H1 associated with H2A-containing nucleosome core particles (NCPs), and H2A.Z-containing NCPs. We find that SUV420H1 makes extensive site-specific contacts with histone and DNA regions. SUV420H1 C-terminal domain recognizes the H2A–H2B acidic patch of NCPs through its two arginine anchors, thus enabling H4K20 insertion for catalysis specifically. We also identify important residues increasing the catalytic activities of SUV420H1 bound to H2A.Z NCPs. In vitro and in vivo functional analyses reveal that multiple disease-associated mutations at the interfaces are essential for its catalytic activity and chromatin state regulation. Together, our study provides molecular insights into the nucleosome-based recognition and methylation mechanisms of SUV420H1, and a structural basis for understanding SUV420H1-related human disease.
A facile dual-template-directed successive assembly approach to hollow multi-shell mesoporous metal–organic framework particles
Authors: Xu Haidong , Han Ji , Zhao Bin , Sun Ruigang , Zhong Guiyuan , Chen Guangrui , Yamauchi Yusuke , Guan Buyuan 
Published: December 2023

AbstractHollow multi-shell mesoporous metal–organic framework (MOF) particles with accessible compartmentalization environments, plentiful heterogeneous interfaces, and abundant framework diversity are expected to hold great potential for catalysis, energy conversion, and biotechnology. However, their synthetic methodology has not yet been established. In this work, a facile dual-template-directed successive assembly approach has been developed for the preparation of monodisperse hollow multi-shell mesoporous MOF (UiO-66-NH2) particles through one-step selective etching of successively grown multi-layer MOFs with alternating two types of mesostructured layers. This strategy enables the preparation of hollow multi-shell mesoporous UiO-66-NH2 nanostructures with controllable shell numbers, accessible mesochannels, large pore volume, tunable shell thickness and chamber sizes. The methodology relies on creating multiple alternating layers of two different mesostructured MOFs via dual-template-directed successive assembly and their difference in framework stability upon chemical etching. Benefiting from the highly accessible Lewis acidic sites and the accumulation of reactants within the multi-compartment architecture, the resultant hollow multi-shell mesoporous UiO-66-NH2 particles exhibit enhanced catalytic activity for CO2 cycloaddition reaction. The dual-template-directed successive assembly strategy paves the way toward the rational construction of elaborate hierarchical MOF nanoarchitectures with specific physical and chemical features for different applications.
An inorganic mineral-based protocell with prebiotic radiation fitness
Authors: Dai Shang , Xie Zhenming , Wang Binqiang , Ye Rui , Ou Xinwen , Wang Chen , Yu Ning , Huang Cheng , Zhao Jie , Cai Chunhui , Zhang Furong , Buratto Damiano , Khan Taimoor , Qiao Yan , Hua Yuejin , Zhou Ruhong , Tian Bing 
Published: December 2023

AbstractProtocell fitness under extreme prebiotic conditions is critical in understanding the origin of life. However, little is known about protocell’s survival and fitness under prebiotic radiations. Here we present a radioresistant protocell model based on assembly of two types of coacervate droplets, which are formed through interactions of inorganic polyphosphate (polyP) with divalent metal cation and cationic tripeptide, respectively. Among the coacervate droplets, only the polyP-Mn droplet is radiotolerant and provides strong protection for recruited proteins. The radiosensitive polyP-tripeptide droplet sequestered with both proteins and DNA could be encapsulated inside the polyP-Mn droplet, and form into a compartmentalized protocell. The protocell protects the inner nucleoid-like condensate through efficient reactive oxygen species’ scavenging capacity of intracellular nonenzymic antioxidants including Mn-phosphate and Mn-peptide. Our results demonstrate a radioresistant protocell model with redox reaction system in response to ionizing radiation, which might enable the protocell fitness to prebiotic radiation on the primitive Earth preceding the emergence of enzyme-based fitness. This protocell might also provide applications in synthetic biology as bioreactor or drug delivery system.
Harmonizing sound and light: X-ray imaging unveils acoustic signatures of stochastic inter-regime instabilities during laser melting
Authors: Hamidi Nasab Milad , Masinelli Giulio , de Formanoir Charlotte , Schlenger Lucas , Van Petegem Steven , Esmaeilzadeh Reza , Wasmer Kilian , Ganvir Ashish , Salminen Antti , Aymanns Florian , Marone Federica , Pandiyan Vigneashwara , Goel Sneha , Logé Roland E. 
Published: December 2023

AbstractLaser powder bed fusion (LPBF) is a metal additive manufacturing technique involving complex interplays between vapor, liquid, and solid phases. Despite LPBF’s advantageous capabilities compared to conventional manufacturing methods, the underlying physical phenomena can result in inter-regime instabilities followed by transitions between conduction and keyhole melting regimes — leading to defects. We investigate these issues through operando synchrotron X-ray imaging synchronized with acoustic emission recording, during the remelting processes of LPBF-produced thin walls, monitoring regime changes occurring under constant laser processing parameters. The collected data show an increment in acoustic signal amplitude when switching from conduction to keyhole regime, which we correlate to changes in laser absorptivity. Moreover, a full correlation between X-ray imaging and the acoustic signals permits the design of a simple filtering algorithm to predict the melting regimes. As a result, conduction, stable keyhole, and unstable keyhole regimes are identified with a time resolution of 100 µs, even under rapid transitions, providing a straightforward method to accurately detect undesired processing regimes without the use of artificial intelligence.
A monoacylglycerol lipase inhibitor showing therapeutic efficacy in mice without central side effects or dependence
Authors: Jiang Ming , Huizenga Mirjam C. W. , Wirt Jonah L. , Paloczi Janos , Amedi Avand , van den Berg Richard J. B. H. N. , Benz Joerg , Collin Ludovic , Deng Hui , Di Xinyu , Driever Wouter F. , Florea Bogdan I. , Grether Uwe , Janssen Antonius P. A. , Hankemeier Thomas , Heitman Laura H. , Lam Tsang-Wai , Mohr Florian , Pavlovic Anto , Ruf Iris , van den Hurk Helma , Stevens Anna F. , van der Vliet Daan , van der Wel Tom , Wittwer Matthias B. , van Boeckel Constant A. A. , Pacher Pal , Hohmann Andrea G. , van der Stelt Mario 
Published: December 2023

AbstractMonoacylglycerol lipase (MAGL) regulates endocannabinoid 2-arachidonoylglycerol (2-AG) and eicosanoid signalling. MAGL inhibition provides therapeutic opportunities but clinical potential is limited by central nervous system (CNS)-mediated side effects. Here, we report the discovery of LEI-515, a peripherally restricted, reversible MAGL inhibitor, using high throughput screening and a medicinal chemistry programme. LEI-515 increased 2-AG levels in peripheral organs, but not mouse brain. LEI-515 attenuated liver necrosis, oxidative stress and inflammation in a CCl4-induced acute liver injury model. LEI-515 suppressed chemotherapy-induced neuropathic nociception in mice without inducing cardinal signs of CB1 activation. Antinociceptive efficacy of LEI-515 was blocked by CB2, but not CB1, antagonists. The CB1 antagonist rimonabant precipitated signs of physical dependence in mice treated chronically with a global MAGL inhibitor (JZL184), and an orthosteric cannabinoid agonist (WIN55,212-2), but not with LEI-515. Our data support targeting peripheral MAGL as a promising therapeutic strategy for developing safe and effective anti-inflammatory and analgesic agents.
Neural substrates of parallel devaluation-sensitive and devaluation-insensitive Pavlovian learning in humans
Authors: Pool Eva R. , Pauli Wolfgang M. , Cross Logan , O’Doherty John P. 
Published: December 2023

AbstractWe aim to differentiate the brain regions involved in the learning and encoding of Pavlovian associations sensitive to changes in outcome value from those that are not sensitive to such changes by combining a learning task with outcome devaluation, eye-tracking, and functional magnetic resonance imaging in humans. Contrary to theoretical expectation, voxels correlating with reward prediction errors in the ventral striatum and subgenual cingulate appear to be sensitive to devaluation. Moreover, regions encoding state prediction errors appear to be devaluation insensitive. We can also distinguish regions encoding predictions about outcome taste identity from predictions about expected spatial location. Regions encoding predictions about taste identity seem devaluation sensitive while those encoding predictions about an outcome’s spatial location seem devaluation insensitive. These findings suggest the existence of multiple and distinct associative mechanisms in the brain and help identify putative neural correlates for the parallel expression of both devaluation sensitive and insensitive conditioned behaviors.