bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATP-Binding Free Energy Simulations Reveal an Allosteric Link Between the Enzyme Active Site and Multiple Functional Protein-Protein Interaction Interfaces in Cyclin Dependent Kinase-1
Authors: Vishwakarma Krishna Kant , Kolthur Ullas Seetharam , Venkatramani Ravindra 
Published: January 2024

AbstractThe ATP dependent phosphorylation activity of Cyclin Dependent Kinase 1 (CDK1), an essential enzyme for cell cycle progression, is intrinsically dependent upon interactions with Cyclin-B, substrate, and Cks proteins. A recent joint experimental-computational study from our group showed intriguingly that acetylation at the active site abrogated the binding of CDK with Cyclin-B. These results posit the possibility of a bi-directional communication between the catalytic site and the protein-protein interface(s). Now, we present evidence for a general allosteric link between the CDK1 active site and all three of its protein-protein interaction (PPI) interfaces through atomistic molecular dynamics simulations (MD). Specifically, we examined ATP binding free energies to CDK1 in native non-acetylated (K33wt) and acetylated (K33Ac) forms as well as in two mutant forms of CDK1, the acetyl-mimic K33Q and the acetyl-null K33R which are accessible in-vitro. We find, in agreement with experiments, that ATP binding is energetically more favorable in K33wt relative to all other states wherein the active site lysine is perturbed (K33Ac, K33Q, and K33R). We also develop an entropy decomposition scheme which reveals, in addition to expected local changes in entropy, significant non-local entropy responses to ATP binding/perturbation of K33 from the 𝛼𝐶-helix, activation loop (A-loop), and the 𝛼𝐺-𝛼H loop segments in CDK1 which interface with Cyclin-B, substrate, and Cks proteins. Statistical analyses over a large set of MD trajectories reveal that while the local and non-local entropic responses to active site perturbations are on average correlated with dynamical changes in the associated protein segments, such correlations may be lost in about 9-48 % of the dataset depending on the segment. Besides, proving the bi-directional communication between the active site and CDK1:Cyclin-B interface, our study provides new insights into the regulation of ATP binding by multiple PPI interfaces in CDK1.
Initial flight test verification of software and hardware in the loop simulations of the flight stabilization system
Authors: Zajdel Albert , Welcer Michal , Szczepanski Cezary Jerzy 
Published: January 2024

Purpose
This paper aims to present assessment of models and simulation results used in the development process of flight stabilisation system that uses trim tabs for PZL-130 Orlik turboprop military trainer aircraft. Flight test of the system allowed to compare software and hardware simulation results with real flight recordings.

Design/methodology/approach
Proposed flight stabilisation system was developed using modern techniques of model-based design, automatic code generation, software and hardware in the loop testing. The project reached flight testing stage which allowed to gather data to verify models and simulation results and asses their quality.

Findings
Results of the comparison showed that the trim tab actuator model used in simulation can be improved by adding play. This reduced the difference between simulation and real flight system output – actuator angle. The influence of airloads on the flying actuator angle compared to hardware in the loop simulation in lab is less than ± 0.6°.

Originality/value
Proposed flight stabilisation system that uses trim tabs has several benefits over classic automatic flight system in terms of weight, energy consumption and structure simplicity and does not need aircraft primary control modification. It was developed using modern techniques of model-based design, automatic code generation and hardware in the loop simulations.
The impact of cooperative communication on co-innovation performance: the mediating role of knowledge sharing
Authors: Fang Wenpei , Wan Liang , Zhou Lei 
Published: January 2024

Purpose
Effective cooperative communication and knowledge management capabilities are particularly important in the cross-team cooperation. Based on the social exchange theory, this paper aims to further explore the role and influence mechanism of cooperative communication and knowledge sharing on co-innovation performance in open innovation projects (OIPs).

Design/methodology/approach
This paper takes China’s high-tech enterprises as the research object by using a paired questionnaire survey based on 168 pairs of OIPs. Hayes’ PROCESS programme and the bootstrap technique are used to test the hypothesis model and the significance of the mediation effect.

Findings
This study finds that cooperative communication is an effective mechanism for the internal and external interaction of OIPs. Knowledge sharing plays an intermediary role in the relationship between cooperative communication and co-innovation performance; both explicit knowledge sharing and tacit knowledge sharing have a significant positive impact on co-innovation performance. And the impact of cooperative communication on co-innovation performance is based on effectively promoting the knowledge sharing of partners.

Originality/value
This research incorporates cooperative communication, knowledge sharing and co-innovation performance into a unified research framework to study the co-innovation at the project level, enriches the research scenario on the mechanism of cooperative behaviour, deepens the understanding of the role and influence mechanism of cooperative communication and knowledge sharing on co-innovation performance in OIPs, verifies the mediating role of knowledge sharing to co-innovation, and also helps extend the social exchange theory to a new research setting.
Online abuse: a systematic literature review and future research agenda
Authors: Saha Raiswa , Ahlawat Sakshi , Akram Umair , Jangbahadur Uttara , Dhaigude Amol S. , Sharma Pooja , Kumar Sarika 
Published: January 2024

Purpose
The study aims to examine the conceptualization of online abuse (OA) and identifies theories, countries of research, top-cited articles, methodologies, antecedents, mediators, outcomes and moderators of OA and future research opportunities. Two research questions are addressed. How have the past studies on OA progressed regarding theories, context, characteristics and methodology? What future research opportunities can be done in this area?

Design/methodology/approach
This study systematically reviews, synthesizes and integrates OA literature using the well-recommended preferred reporting items for systematic reviews and meta-analyses (PRISMA) rules. The literature on OA was synthesized based on the Theory–Context–Characteristics–Methodologies (TCCM) framework given by Paul and Rosado-Serrano.

Findings
Through an examination of TCCM used in OA research, the review presents an all-inclusive and up-to-date overview of the research in this arena and sets a future research agenda to spur scholarly research. This systematic literature review has analyzed top-quality sample papers, published in the past decade. As a result, it contributes to a better understanding of this relationship by analyzing the different types of use and the value added to the shopping experience.

Originality/value
This study provides groundwork for researchers and promotes a deeper understanding of OA.
How perceived crowding changes the interrelationships between perceived value, tourist satisfaction and destination loyalty: the empirical study at Hoi An
Authors: Phi Long Nguyen , Phuong Dung Hoang , Huy Thong Vu 
Published: January 2024

Purpose
This paper seeks to revisit the interrelationship among tourists’ perceived value of the destination, tourist satisfaction and destination loyalty in the heritage tourism site of Hoi An. In addition, the moderating role of tourists’ perceived crowding, which has become remarkably common at the site, in such a triangle relationship will also be explored. In other words, this study aims to validate an extended model of perceived value – tourist satisfaction – destination loyalty – perceived crowding.

Design/methodology/approach
The study collects data from 403 tourists who visited Hoi An during peak season through an online questionnaire. The data were later analysed using AMOS and Warp partial least squares.

Findings
The results validate the significant and positive correlation among perceived value, customer satisfaction and destination loyalty. Also, perceived crowding was confirmed to affect the relationship among these three variables negatively. In terms of academic contributions, this paper empirically proved that low levels of tourist satisfaction and destination loyalty among tourists who highly value their visiting experience at World Heritage Sites (WHS) can be caused by perceived crowding.

Originality/value
So far, current literature has investigated the direct (either positive or negative) relationship between perceived crowding and post-visit behaviours of tourists (Nie et al., 2022; Papadopoulou, Ribeiro, & Prayag, 2023; Stemmer, Gjerald, & Øgaard, 2022). Broadening this area of research, the authors of this paper used the social interference theory and the stimulus-overload theory to explain the low level of tourist satisfaction and destination loyalty among tourists who highly value their visiting experience at WHS.
Utilizing functional genomics in Saccharomyces cerevisiae to characterize food preservative compounds: A pilot study
Authors: Smith Jeremy R. , Martini Austin , Gibney Patrick A. 
Published: January 2024

AbstractChemical preservatives are ubiquitously used to suppress the growth of or kill microorganisms across numerous industries, including the food industry. Utilizing yeast functional genomic techniques, genes and their functions can be observed at a genomic scale to elucidate how environmental stressors (e.g., chemical preservatives) impact microbial survival. These types of chemical genomics approaches can reveal genetic mutations that result in preservative resistance or sensitivity, assist in identification of preservative mechanism of action, and can be used to compare different preservatives for rational design of preservative mixtures. In this proof‐of‐concept study, we performed deletion and high‐copy genetic expression screens to identify mutants that confer drug resistance to sodium benzoate, potassium sorbate, rosemary extract, and Natamax. By observing overlapping mutant genes between genetic screens, we were able to identify functional overlap between chemical preservatives and begin to explain mechanisms of action for these compounds.
Rechallenge of immune checkpoint inhibitors in advanced non‐small cell lung cancer
Authors: Lin Gen , Wang Zhijie , Chu Qian , Hu Yi , Huang Dingzhi , Wang Jun , Yang Fan , Zhong Wenzhao , Zhou Chengzhi , Zhu Bo , Ai Xinghao , Cao Baoshan , Cao Yabing , Chen Mingqiu , Chen Xiaohui , Chu Tianqing , Duan Jianchun , Fan Yun , Fang Yong , Feng Shuitu , Feng Weineng , Guo Hui , Han Chengbo , He Yong , Hong Shaodong , Hu Jie , Huang Meijuan , Huang Yan , Jiang Da , Jiang Kan , Jiang Richeng , Jin Bo , Jin Shi , Li Jisheng , Li Min , Li Ziming , Li Chao , Lin Jie , Liu Anwen , Liu Si‐Yang Maggie , Yutao Liu , Liu Zhefeng , Liu Zhe , Liu Zhenhua , Liu Zhentian , Liu Zhigang , Lu Yuping , Lv Tangfeng , Ma Zhiyong , Miao Qian , Peng Min , Pu Xingxiang , Ren Xiu Bao , Shan Jianzhen , Shan Jinlu , Shen Peng , Shen Bo , Shi Meiqi , Song Yong , Song Zhengbo , Su ChunXia , Sun Jianguo , Tian Panwen , Wang Jinliang , Wang Feng , Wang Huijuan , Wang Jialei , Wang Qian , Wang Wenxian , Wang Yan , Wu Lin , Wu Fang , Xia Yang , Xie Congying , Xie Conghua , Xin Tao , Xiong Jianping , Xu Haipeng , Xu Song , Xu Yiquan , Xu Bin , Xu Chunwei , Yan Xiaolong , Yang Zhenzhou , Yao Wenxiu , Yu Yao , Feng Ye , Yu Zongyang , Yu Yongfeng , Yue Dongsheng , Zhang Haibo , Zhang HongMei , Zhang Li , Zhang Longfeng , Zhang Qiuyu , Zhang Tongmei , Zhang Bicheng , Zhao Jun , Zhao Mingfang , Zheng Xiaobin , Zhong Qiaofeng , Zhou Jin , Zhou Penghui , Zhu Zhengfei , Zou Juntao , Zou Zihua 
Published: January 2024

AbstractImmune checkpoint inhibitor (ICI) rechallenge in non‐small cell lung cancer (NSCLC) is a promising therapeutic strategy. The situation for ICI rechallenge can be divided into three categories: adverse events (AEs); resistance to ICIs, and rechallenge becomes compulsive because of tumor relapse while the patients had completed a 2 year course of immunotherapy. However, these categories are still controversial and should be explored further. Through voting at the 6th Straits Summit Forum on Lung Cancer, in this study we summarize the consensus of 147 experts in ICI rechallenges. A total of 97.74% experts agreed to rechallenge; 48.87% experts rechallenge with the original drug, and the others rechallenge with a different drug; 40.3% agreed to rechallenge directly after progression; 88.06% experts agreed to ICI rechallenge with a combination regimen; and factors such as previous performance status score, PD‐1 expression, and age should also be considered. Understanding the the clinical studies in ICI rechallenge could bring us one step closer to understanding the consensus. In patients with advanced NSCLC who have suffered recurrent or distant metastasis after immunotherapy, the option of rechallenge with ICIs is a promising treatment option.
Digitalization amidst educational crises: A comparative study of the new media literacy (NML) levels of the Philippines and Myanmar
Authors: Maningo Clyde A. , Hlaing Sa Phyo A. M. 
Published: January 2024

AbstractWhile traditional media literacy (TML) facilitates outdated patterns of digital media response, new media literacy (NML) frames the adaptation of literacy to the ever‐dynamic trend of media development. It then raises the cruciality of integrating NML concepts into national educational frameworks. The study aims to explore the case of Myanmar and the Philippines' NML capacity levels. Both countries have a digital media environment enveloped by information distortions mainly rooted in tumultuous political climates. The study leans to notions of educational crises in both countries as seen in terms of (1) sociopolitical contexts that lead to the irresponsive initiatives to improve individual criticality, (2) lack of emphasis on NML response, and (3) the implication of state and individual approaches against false information. Finally, the study presents a multifaceted response to educational paradigms derived from interpretive social‐scientific approaches in seeing the critical issues concerning digital media use.
How the state frames its engagement in renminbi internationalization: The case of Indonesia
Authors: Mellynia  , Karim Moch Faisal 
Published: January 2024

AbstractThe rise of China has accelerated Renminbi (RMB) internationalization. While there seems to be global support for RMB internationalization, there is a gap in understanding how developing nations, facing potential domestic skepticism, rationalize and articulate their support. This article discusses narratives that states use to legitimize their support for RMB internationalization. Using the case of Indonesia, this article examines the country's policy responses to China's RMB internationalization strategy. It uncovers that Indonesia frames its engagement with RMB internationalization not merely as an endorsement of China's global ambitions but as a strategic move to hedge against US dollar vulnerability and to foster broader Asian regional integration, particularly in terms of currency cooperation. This article enriches the existing literature, providing a nuanced perspective on how states navigate the complexities of international economic diplomacy, crafting narratives that align with their strategic interests and policy objectives in the evolving landscape of global currency politics.
Dapsone for paediatric chronic immune thrombocytopenia: Short report from a tertiary centre in South India
Authors: Bharadwaj Niteesh , Munireddy Jyothi , Selvam Sumithra , Bharadwaj Vandana , Prakash Anand 
Published: January 2024

SummaryImmune thrombocytopenia (ITP) resolves in most children within 3–12 months of diagnosis. Chronic ITP affects 10%–20% of patients, some of whom require treatment. Several second‐line agents are efficacious in this group of patients. This paper describes our experience of using dapsone as a single second‐line agent in children with chronic ITP. One hundred and three children with chronic ITP were seen at our centre from January 2012 to December 2016. Forty‐five children met the inclusion criteria and received dapsone; 17 (37.8%) were boys; and 28 (62.2%) were girls. Early response to dapsone was seen in 37.8% of patients. The median duration of long‐term follow‐up was 50 months, and at least a partial response was seen in 64.4% of the patients. Dapsone offers good initial response rates and sustained remission in paediatric chronic ITP, comparable to other therapeutic agents available.
Hao‐Fountain syndrome: 32 novel patients reveal new insights into the clinical spectrum
Authors: Wimmer Moritz Claudius , Brennenstuhl Heiko , Hirsch Steffen , Dötsch Laura , Unser Samy , Caro Pilar , Schaaf Christian Patrick 
Published: January 2024

AbstractHao‐Fountain syndrome (HAFOUS, OMIM: #616863) is a neurodevelopmental disorder caused by pathogenic variants in the gene USP7 coding for USP7, a protein involved in several crucial cellular homeostatic mechanisms and the recently described MUST complex. The phenotype of HAFOUS is insufficiently understood, yet there is a great need to better understand the spectrum of disease, genotype–phenotype correlations, and disease trajectories. We now present a larger cohort of 32 additional individuals and provide further clinical information about six previously reported individuals. A questionnaire‐based study was performed to characterize the phenotype of Hao‐Fountain syndrome more clearly, to highlight new traits, and to better distinguish the disease from related neurodevelopmental disorders. In addition to confirming previously described features, we report hyperphagia and increased body weight in a subset of individuals. HAFOUS patients present an increased rate of birth complications, congenital anomalies, and abnormal pain thresholds. Speech impairment emerges as a potential hallmark of Hao‐Fountain syndrome. Cognitive testing reports reveal borderline intellectual functioning on average, although some individuals score in the range of intellectual disability. Finally, we created a syndrome‐specific severity score. This score neither indicates a sex‐ nor age‐specific difference of clinical severity, yet highlights a more severe outcome when amino acid changes colocalize to the catalytic domain of the USP7 protein.
Temporal association between sleep spindles and ripples in the human anterior and mediodorsal thalamus
Authors: Szalárdy Orsolya , Simor Péter , Ujma Péter Przemyslaw , Jordán Zsófia , Halász László , Erőss Loránd , Fabó Dániel , Bódizs Róbert 
Published: January 2024

AbstractSleep spindles are major oscillatory components of Non‐Rapid Eye Movement (NREM) sleep, reflecting hyperpolarization‐rebound sequences of thalamocortical neurons. Reports suggest a link between sleep spindles and several forms of high‐frequency oscillations which are considered as expressions of pathological off‐line neural plasticity in the central nervous system. Here we investigated the relationship between thalamic sleep spindles and ripples in the anterior and mediodorsal nuclei (ANT and MD) of epilepsy patients. Whole‐night LFP from the ANT and MD were co‐registered with scalp EEG/polysomnography by using externalized leads in 15 epilepsy patients undergoing a Deep Brain Stimulation protocol. Slow (~12 Hz) and fast (~14 Hz) sleep spindles were present in the human ANT and MD and roughly, 20% of them were associated with ripples. Ripple‐associated thalamic sleep spindles were characterized by longer duration and exceeded pure spindles in terms of spindle power as indicated by time‐frequency analysis. Furthermore, ripple amplitude was modulated by the phase of sleep spindles within both thalamic nuclei. No signs of pathological processes were correlated with measures of ripple and spindle association, furthermore, the density of ripple‐associated sleep spindles in the ANT showed a positive correlation with verbal comprehension. Our findings indicate the involvement of the human thalamus in coalescent spindle‐ripple oscillations of NREM sleep.
Biochemical, biomechanical and imaging biomarkers of ischemic stroke: Time for integrative thinking
Authors: Erdoğan Meryem Şahin , Arpak Esra Sümer , Keles Cemre Su Kaya , Villagra Federico , Işık Esin Öztürk , Afşar Nazire , Yucesoy Can A. , Mur Luis A. J. , Akanyeti Otar , Saybaşılı Hale 
Published: January 2024

AbstractStroke is one of the leading causes of adult disability affecting millions of people worldwide. Post‐stroke cognitive and motor impairments diminish quality of life and functional independence. There is an increased risk of having a second stroke and developing secondary conditions with long‐term social and economic impacts. With increasing number of stroke incidents, shortage of medical professionals and limited budgets, health services are struggling to provide a care that can break the vicious cycle of stroke. Effective post‐stroke recovery hinges on holistic, integrative and personalized care starting from improved diagnosis and treatment in clinics to continuous rehabilitation and support in the community. To improve stroke care pathways, there have been growing efforts in discovering biomarkers that can provide valuable insights into the neural, physiological and biomechanical consequences of stroke and how patients respond to new interventions. In this review paper, we aim to summarize recent biomarker discovery research focusing on three modalities (brain imaging, blood sampling and gait assessments), look at some established and forthcoming biomarkers, and discuss their usefulness and complementarity within the context of comprehensive stroke care. We also emphasize the importance of biomarker guided personalized interventions to enhance stroke treatment and post‐stroke recovery.
Supply contracts under partial forward ownership
Authors: Hunold Matthias , Schlütter Frank 
Published: January 2024

AbstractWith forward ownership, an upstream supplier internalizes the effect of its supply contracts on the downstream firms, which is so far understood to decrease prices. We show that, instead, downstream prices generally increase if firms use two‐part tariffs. The price‐increasing effect of forward ownership occurs with both observable and secret two‐part tariffs, albeit for different economic reasons. The results arise under both quantity and price competition as well as for different belief refinements. Partial forward ownership can be more profitable and more harmful for consumers than a full vertical merger between an upstream and a downstream firm.