bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal Transcriptomics of Human Cardiovascular Progenitors in Pig Hearts Identifies MIDKINE as a Positive Regulator of Neovascularization
Authors: Yap Lynn , Adusumalli Swarnaseetha , Lim Samantha , Ren Vincent , Leong Kye Siong , Chong Li Yen , Tan Clarissa , Tham Roy , Cheng Min En , Ye Lei , Wang Yibin , Petretto Enrico , Tryggvason Karl 
Published: January 2025

Abstract

Stem cell-based therapy has shown promise for regenerating infarcted heart tissue, yet the molecular dynamics and cellular fate of transplanted cells within the myocardium remain largely unexplored. In this study, we used time-series spatial transcriptomics to analyze the global gene expression profile of human pluripotent stem cell-derived cardiovascular progenitors (CVPs) transplanted into pigs at 1, 4, and 12 weeks post-chronic myocardial infarction (CMI). We employed bioinformatics tools, including ligand-receptor interaction and signaling pathway analysis, and identified MIDKINE (MDK), a human xenograft-derived chemokine, as a key regulator of neovascularization. These findings were validated through downstream bioinformatics analysis, immunohistochemistry, and in vitro genetic manipulation.
Our results revealed important cardiac repair mechanisms, such as increased expression of markers associated with cardiac maturation, including genes related to metabolism, cell cycle regulation, calcium handling, sarcomere development, ribosome biogenesis, and fibrosis resolution. Notably, through ligand-receptor interaction analysis, we identified the ligand MDK as a promising target to enhance angiogenic responses in the pig heart following cellular transplantation. This provides a valuable foundation for understanding the mechanisms of cellular therapy in the context of degenerative heart disease.
To further support our findings, we generated MDK-overexpressing cardiomyocytes and observed a significant increase in endothelial cell migration in transwell assays compared to controls (P-value < 0.05). For a deeper exploration of our transcriptomic data, we developed a spatially- and time-resolved transcriptomic Shiny application atlas of CVP xenografts in MI pig hearts during the critical post-transplantation recovery phase, enabling interactive exploration of gene expression profiles from our large animal models.
Hyperpolarized [1- 13 C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in tiny multicellular spheroids
Authors: Takakusagi Yoichi , Takakusagi Kaori , Inoue Kaori , Saito Keita , Homma Yoshimi , Ichikawa Kazuhiro 
Published: January 2025

Abstract

Hyperpolarized (HP) [1-13C]pyruvate nuclear magnetic resonance (NMR) spectroscopy was employed to investigate tumor energy metabolism in tiny multicellular spheroids, serving as a model of early-phase tumorigenesis in vivo. A three-dimensional static culture of murine squamous cell carcinoma (SCCVII) cells formed uniform smaller multicellular spheroids (~ 150 µm in diameter), without hypoxic or necrotic cores, yet these spheroids exhibited resistance to anti-tumor drugs. HP [1-13C]pyruvate NMR spectroscopy of SCCVII spheroids revealed an increased conversion of pyruvate to lactate compared to monolayer cultures, indicating enhanced aerobic glycolysis in the aggregated cells. Additionally, HP spectroscopy differentiated the degree of aerobic glycolysis in human prostate tumor spheroids―DU145 (~ 120 µm) and PC-3 (~ 230 µm)―as evidenced by the upregulation of genes associated with lactate production and cellular transport. The Lac/Pyr ratio among spheroids correlated with those observed in biopsy samples of corresponding malignant tumors grown in mice. These findings suggest that HP [1-13C]pyruvate NMR spectroscopy may serve as a metabolic biomarker for early-phase tumorigenesis in vivo.
Blood Glucose-Lowering Effect of Hydroxychloroquine Sulfate in Systemic Lupus Erythematosus: A Quantitative Analysis
Authors: Zhang Li-Li , Shu Hua , Guo XiangHua , Zhang XinShuai , Dong GuanJun , Song Qin 
Published: January 2025

Abstract

Objective
The objective of this study was to quantitatively evaluate the effect of hydroxychloroquine sulfate on fasting blood glucose levels in patients diagnosed with systemic lupus erythematosus.
Methods
A retrospective cohort study was conducted by reviewing the medical records of 905 SLE patients between January 2020 and December 2024. The study compared fasting blood glucose (FBG) levels between 255 patients who received hydroxychloroquine (HCQ) treatment and 650 patients who did not receive HCQ during the study period. Multivariate regression, stratified analysis, and smooth curve fitting were employed to assess the relationship between HCQ use and glucose levels.
Results
Following the adjustment of confounding factors, a mean reduction of 0.2 mmol/L in FBG was observed among HCQ users in comparison to non-users (95%CI: -0.4, -0.0, P = 0.0294). A stratified analysis revealed that this effect was more pronounced in female patients. Furthermore, the analysis of the glucose levels in relation to age demonstrated that they were more stable in individuals who had been administered HCQ.
Conclusion
This study provides the first quantitative evidence of HCQ's significant glucose-lowering effect in SLE patients, offering important implications for clinical practice.
Antimicrobial effect of advanced platelet-rich fibrin plus against methicillin-susceptible and methicillin-resistant Staphylococcus aureus: An in vitro study
Authors: Le Son , Le-Nguyen Minh-Phuc , Pham Uy , Nguyen Bich-Ly 
Published: January 2025

Abstract

Background
The virulence of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) differs significantly; however, the antimicrobial effects of advanced platelet-rich fibrin plus (A-PRF+) on these subspecies remain unclear. This study aimed to evaluate the efficacy of A-PRF + against MSSA and MRSA.
Methods
Fifteen male participants volunteered for this study. Solid and liquid forms of A-PRF + were produced using the DUO Quattro centrifuge machine following the recommended protocol. The inoculum of MSSA and MRSA was prepared from reference samples obtained from the American Type Culture Collection. Both inocula were adjusted to a McFarland standard of 0.5. The antimicrobial effects of A-PRF + against MSSA and MRSA were evaluated using disk diffusion assays, minimum inhibitory concentration (MIC) tests, and biofilm formation experiments.
Results
The disk diffusion assay demonstrated weak antimicrobial activity against both MSSA and MRSA, with inhibition zones measuring 0.68 ± 0.44 mm and 0.69 ± 0.35 mm, respectively. However, MIC testing revealed that A-PRF + did not exhibit antimicrobial effects against either subspecies following dilution. Finally, A-PRF + significantly reduced the biofilm-forming capacity of both MSSA and MRSA to approximately 70%.
Conclusion
A-PRF + exhibited weak antimicrobial activity against both MSSA and MRSA in agar diffusion assays. Additionally, A-PRF + reduced the biofilm-forming capacity of both MSSA and MRSA. However, no significant differences were detected in the antimicrobial effects of A-PRF + between MSSA and MRSA.
Anthropogenic metal storage in wetland soils across the conterminous United States
Authors: Dietrich Matthew , Dumelle Michael , Nahlik Amanda M. , Golden Heather E. , Christensen Jay R. , Lane Charles R. , Moore Eric M. , Filippelli Gabriel M. 
Published: January 2025

Abstract

Wetlands provide many ecosystem services, such as mitigating pollution, attenuating flooding and drought extremes, and providing habitat for many species. However, studies quantifying potential wetland sequestration of heavy metals as an ecosystem service, particularly across large spatial extents, are sparse. We utilized data from the United States Environmental Protection Agency’s National Wetland Condition Assessment to estimate anthropogenic metal (Pb, Cu, Cr) storage in the upper 40 centimeters of wetland soils across the conterminous United States (CONUS). Large amounts of anthropogenic Cu and Cr are stored in wetland soil across the CONUS, at 299.5 ± 73.2 (95% confidence interval) and 483.4 ± 132.1 thousand metric tons (MT), respectively. Anthropogenic Pb totaled 394.3 ± 265.2 thousand MT, an amount roughly equivalent to 7% of lead-based gasoline additives used in the U.S. between 1927–1994, the largest widespread anthropogenic source of Pb across the landscape. Between 15–22% of Cu, Cr, and Pb mass stored within the upper 40 cm of wetland soils across the CONUS is anthropogenic. We also estimated wetland anthropogenic metal loading to normalize mass by area. Anthropogenic metal loading is related to the complex interaction of landscape features. However, national-scale variations obscure which features are dominant in metal loading and retention processes. In most cases, estimated wetland redox state, tidal influence, the wetland hydrologic regime, and the geographical region do not substantially impact estimates of anthropogenic metal loading within wetland soils. More detailed regional research may help disentangle these complex relationships to further support wetland ecosystem management.
Synthesis of polyethersulfone membranes with the addition of silver nitrate for water filter applications
Authors: Firdaus Aneka , Nasir Subriyer , Dani Rahma , Prasatya Iqbal Nova , Ismail Ahmad Fauzi , Othman Mohd Hafiz Dzarfan , Mataram Agung 
Published: January 2025

Clean water is a fundamental need in human life. Clean water is becoming limited due to increasing population growth and pollution. On the other hand, environmental damage due to domestic and factory liquid waste disposal is also a factor that makes the scarcity of clean water sources. Water purification is essential to maintain the sustainability of human life. Polyethersulfone (PES) polymer-based membrane technology is one of the approaches used to overcome this problem. To be effective as a water filtration membrane, PES is added with Silver Nitrate (AgNO3) for antibacterial purposes and to reduce the fouling effect. This study looks at different concentrations of silver nitrate in increasing the flux of PES-based membranes. The method used was electric field treatment to modify the membrane surface. SEM results show that PES/AgN03 membranes PA1, PA2, and PA3 produce porous, smooth, thin, densely arranged structures with typical finger-like pore sub-structures that correspond to PES characteristics. While testing mechanical properties resulted in the membrane PA1 having a strength of 5.74206 MPa, PA2 with a tensile strength of 3.81310 MPa, and PA3 having a tensile strength of 3.07480 MPa showing that the increasing AgNo3 decreased the tensile strength of the membrane. The wettability of membranes ranging between 135.2°, 137.6°, and 140.4° implies that due to their hydrophilicity, PES/AgNo3 membranes absorb more contaminants and impurity particles. CWP testing showed that the membrane flux ranged from 3.88797 L.m-2.h-1.bar-1, 3.69523 L.m-2.h-1.bar-1, and 3.01345 L.m-2.h-1.bar-1. Based on the test results, PES membrane added with silver nitrate with accurate composition has potential in water filtration applications
Оценка гликемии и потребности в глюкозе у новорожденных детей во время оперативного вмешательства: проспективное клиническое исследование
Authors: Нассер М. М. , Жиркова Юлия Викторовна , Кучеров Ю. И. , Межевикина В. М. 
Published: January 2025

INTRODUCTION: Despite numerous studies and the existence of many recommendations regarding infusion therapy in newborns, the need for subsidies and precise dosing of intraoperative glucose for children of the neonatal period has not been determined. OBJECTIVES: Analysis of the intraoperative glycemic profile in newborns and determination of the need for parenteral glucose subsidies during surgery in this category of patients. MATERIAL AND METHODS: The study included 101 newborn children under 28 days of age with surgical diseases requiring surgical treatment in the neonatal period. The study was approved by the local ethics committee of the Russian National Research Medical University named after. N.I. Pirogov reference number 192 dated January 27, 2020. Demographic and clinical data were assessed using nonparametric statistics and presented as median values with interquartile ranges. Continuous variables were compared using one-way analysis of variance. RESULTS: In the absence of a planned subsidy of glucose intraoperatively, 74.5 % of children have hypoglycemia < 2.9 mmol/l, 55.3 % have blood sugar < 2.6 mmol/l, especially at the stages of intubation and skin incision. With planned titration of glucose at a dose of 2.3 mg/kg/min, hypoglycemia is recorded almost 2 times less often (40 % of children) (p = < 0.001), a third of children had hyperglycemia (16.7 %) and a hyperosmolar state (50 %). When glucose was administered at a dose of 1.15 mg/kg/min, more children had euglycemia of 55 %, p = 0.038. CONCLUSIONS: A statistically significant difference in glycemia during surgery in children was revealed depending on the intraoperative glucose supplement. The need of glucose is individual and depends on many factors.
Модуляция метаболизма микробиоты у кардиохирургических пациентов: рандомизированное контролируемое исследование
Authors: Грекова М. С. , Белобородова Н. В. , Паутова А. К. , Гринь О. О. , Кабанова Т. А. , Дымова О. В. , Еременко А. А. , Бабаев Максим Александрович 
Published: January 2025

INTRODUCTION: Microbiota metabolism disturbance, assessed by the dynamics of clinically significant aromatic metabolites in the blood, plays an important role in the development of complications after cardiac surgery, which is the basis for the search for a new prevention strategy. OBJECTIVES: To evaluate the feasibility of microbial metabolism modulation using antibiotics-inhibitors of protein synthesis in bacterial cell for prevention of infectious-inflammatory complications after cardiac surgery. MATERIAL AND METHODS: A randomized prospective interventional study was conducted, including patients (n = 58) who underwent planned surgical intervention on the heart and main vessels under artificial circulation and were divided into group I — main group — patients with microbial metabolism modulation using antibiotics-inhibitors of protein synthesis (n = 30), group II — control group — patients without microbial metabolism modulation (n = 28). Blood serum samples from patients were taken before surgery, on the 3rd and 6th days of the postoperative period, in which clinical blood parameters, biomarkers, and concentrations of clinically significant aromatic acids were determined. RESULTS: In groups I and II postoperative complications were observed in 6 and 8 patients, respectively. Groups I and II differed significantly in the frequency of pneumonia (0 vs. 5 patients, respectively, p = 0.016). The spectrum of microorganisms determined in group II was significantly wider. In group I there was a more significant decrease in 4-hydroxyphenylacetic acid (p-HPhAA) concentration between baseline and postoperative values on the 3rd and 6th day, compared to group II (p = 0.016 and p = 0.005, respectively). The sum of aromatic metabolites on the 3rd day and p-HPhAA on the 6th day had a good predictor significance in relation to the complication development (sensitivity 100 % in both cases; specificity 81.5 % and 92.6 %, respectively). CONCLUSIONS: Prophylactic use of antibiotics-inhibitors of microbial protein synthesis allows to reliably reduce the degree of microbiota metabolism dysfunction and the frequency of pneumonia development in the early postoperative period.
Modern view on the problem of therapy of recurrent polyposis rhinosinusitis, comparative study of the effectiveness of different monoclonal antibody preparations
Authors: Kryukov A. I. , Gurov A. V. , Fomina D. S. , Tovmasyan A. S. , Rabadanov G. K. 
Published: January 2025

Introduction. The main methods of treatment of patients with chronic polyposis rhinosinusitis are surgical and conservative, despite the significant successes achieved in the treatment of the polyposis process, epidemiologic and clinical studies show that there is no complete control of this disease. Taking into account the pros and cons of the above-mentioned methods of treatment, it seems relevant for the scientific community to further search for new methods that will combine high efficacy and low probability of developing undesirable systemic manifestations. One of such methods is targeted biological therapy with the use of monoclonal antibodies.Aim. To evaluate the efficacy of monoclonal antibodies in the therapy of patients with polyposis rhinosinusitis.Materials and methods. The authors studied the outpatient records of 102 patients aged 20 to 87 years, in whose therapy monoclonal antibodies (dupilumab, omalizumab, mepolizumab) were used. All patients participating had concomitant pathology in the form of chronic recurrent polyposis rhinosinusitis with the main disease – bronchial asthma. The subjects were divided into 3 groups, in the treatment of which one of the monoclonal antibody preparations was used.Results and discussion. Regression of the main symptoms of chronic polyposis rhinosinusitis (loss of sense of smell and taste, pain/pressure in the facial area, reduction of runny nose, mucus flowing down the posterior pharyngeal wall, nasal congestion) was observed in each of the 3 groups against the background of therapy with monoclonal antibodies. The effectiveness of the therapy was evaluated according to the results of endoscopic picture (Lund-Kennedy scale), CT picture (Lund-Mackay scale) and the data of subjective questionnaire SNOT-22.Conclusion. The studied molecules showed efficacy in the therapy of chronic polyposis rhinosinusitis. However, dupilumab was the most preferable molecule, the use of which allows to achieve more pronounced regression of the main clinical symptomatology, to reduce the degree of recurrence of the polyposis process with the absence of the need for repeated surgical interventions, as well as allows to improve the quality of life of patients.
Association of genes CSK, MTHFR, АСЕ, ADRA2B, TCF7L2 with metabolic syndrome in indigenous and non-indigenous young residents of Western Siberia
Authors: Korneeva E. V. , Voevoda M. I. , Maksimov V. N. , Semaev S. E. 
Published: January 2025

Introduction. Due to the negative trend of growth of cardiovascular and endocrine diseases among young people, the need to study the genes involved in the development of metabolic disorders is becoming urgent.Aim. To study the prevalence of variants of genes CSK, MTHFR, ACE, ADRA2B, TCF7L2 and their association with metabolic syndrome in indigenous and non-indigenous men and women living in the Khanty-Mansiysk autonomous okrug – Yugra. Materials and methods. The study involved 863 people living in the city of Surgut and in the Surgut district of the KhantyMansiysk autonomous okrug – Yugra aged 18 to 44 years. The indigenous population (280 people) is represented by 76 men and 204 women, the non–indigenous population (583 people) is represented by 207 men and 376 women. The DNA of genes CSK, ACE, ADRA2B, MTHFR and TCF7L2 was isolated by polymerase chain reaction.Results. It was found that among indigenous (khanty) and non-indigenous men and women there are some differences in the prevalence of alleles and genotypes of gene variants in comparison with the international GnomAD database (v.3.1), which is associated with the peculiarities of ethnic composition and environment. The association of the rs1799752 variant of the gene ACE with metabolic syndrome was found in non-indigenous people: in women with the genotype ID (p = 0.027), in men with the genotype DD (p = 0.019).Conclusion. Thus, there were no statistically significant differences between indigenous and non-indigenous residents in the frequency of alleles and genotypes of variants rs1378942 of the gene CSK, rs1801133 of the gene MTHFR, rs1799752 of the gene ACE, rs28365031 of the gene ADRA2B and rs7903146 of the gene TCF7L2. In the group of non-indigenous residents, the presence of metabolic syndrome is more often associated with the carrier variant rs1799752 of the gene ACE. The research results can be used to develop individual approaches to treatment and prevention that take into account the genetic characteristics of each person.
Effectiveness of control of edema in patients with obesity and lymphedema after mastectomy using bioimpedancemetry
Authors: Konchugova T. V. , Apkhanova T. V. , Vasileva V. A. , Marchenkova L. A. , Kulchitskaya D. B. , Marfina T. V. , Mukhina A. A. 
Published: January 2025

Introduction. Control of edema in obese patients with postmastectomy lymphedema is an important aspect of care for patients undergoing curative treatment for breast cancer. One effective and highly sensitive method for monitoring edema is bioimpedance measurement, which can be a useful tool for assessing tissue and fluid balance in such patients.Aim. To study the feasibility of using bioimpedance analysis as a method to assess the efficacy of the anti-edema effects of physiotherapeutic treatment methods in patients with secondary lymphedema after mastectomy.Materials and methods. In this review, a search for sources describing the effectiveness of the bioimpedanceometry method for controlling edema in patients with secondary lymphedema after mastectomy for breast cancer was carried out in several English text databases: PubMed, Scopus, Web of Science, Springer Link, and in the scientific electronic library elibrary.ru.Results. After mastectomy, approximately 18% of patients undergoing radical treatment for breast cancer experience secondary lymphedema due to impaired lymphatic drainage of the upper extremity on the affected side as a result of lymph node removal. The development of lymphedema of the upper limb is accompanied by a significant decrease in the quality of life of patients and requires constant medical monitoring. Bioimpedansometry allows you to estimate the volume of fluid in the body and determine the presence of edema based on changes in the electrical resistance of tissues. In obese patients with lymphedema following mastectomy, this method may be particularly useful for early detection of subclinical limb lymphedema and evaluation of treatment effectiveness.Conclusions. Thus, the use of bioimpedance measurements in the practice of oncologists and rehabilitation specialists can significantly facilitate the control of edema in patients with obesity and lymphedema after mastectomy. This method provides objective data on the state of fluid balance and allows the detection of edema in the early stages, which facilitates the earlier start of intensive non-pharmacological interventions to increase the effectiveness of postoperative rehabilitation and improve the quality of life of patients.
Calibrating the Priestley–Taylor model for evapotranspiration across different substrate depths in green roofs
Authors: Xie Haowen , Wu Yawen , Randall Mark 
Published: January 2025

ABSTRACT

This study aims to address a common issue in current research: the neglect of the calibrating model parameters when estimating evapotranspiration (ET) from green roofs (GRs) using the Priestley–Taylor model, with most studies limited to a single substrate depth (SD). To overcome this limitation, this research improves the accuracy of ET estimation for different SDs on GRs by calibrating the Priestley–Taylor coefficient α. The study period was 692 days in total, from 25 April 2021 to 26 April 2023. Daily ET data from the outdoor GR experimental group were used to calibrate and validate the model. Uncalibrated models perform well for medium SDs (150 mm) but decline for deeper (300 mm) or shallower (50 mm) ones. NSGA-II optimization significantly improved model performance across all SDs, notably at 300 mm. The research underscores the importance of parameter calibration for water management in GRs and sets a foundation for future research on optimizing water retention and regulation functions in GRs.