bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidy
Authors: Hintzen Dorine C. , Schubert Michael , Soto Mar , Medema René H. , Raaijmakers Jonne A. 
Published: December 2023

AbstractAneuploidy, while detrimental to untransformed cells, is notably prevalent in cancer cells. This indicates that cancer cells have the ability to surmount the initial stress responses associated with aneuploidy, enabling rapid proliferation despite aberrant karyotypes. To generate more insight into key processes and requirements underlying the adaptation to aneuploidy, we generated a panel of aneuploid clones in p53-deficient RPE-1 cells and studied their behavior over time. As expected,de novogenerated aneuploid clones initially displayed reduced fitness, enhanced levels of chromosomal instability and an upregulated inflammatory response. Intriguingly, after a prolonged period of culturing, aneuploid clones exhibited increased proliferation rates while maintaining aberrant karyotypes, indicative of an adaptive response to the aneuploid state. Interestingly, all adapted clones displayed reduced chromosomal instability (CIN) and reduced inflammatory signaling, suggesting that these are common aspects of adaptation to aneuploidy. Collectively, our data suggests that CIN and concomitant inflammation are key processes that require correction to allow for fast growth. Finally, we provide evidence that amplification of oncogenic KRAS can promote adaptation.
Product-based marketing strategies: pork consumption in Taiwan and Japan
Authors: Ip Ching Yin , Liang Chaoyun 
Published: December 2023

PurposeThe present study surveyed consumers in Taiwan and Japan to analyse the influence of marketing mix on purchase intention and the willingness to pay for Taiwanese pork and to establish a marketing-oriented model of consumer behaviour.Design/methodology/approachA total of 1,134 valid Internet surveys were collected, which included 526 Taiwanese respondents and 608 Japanese respondents.FindingsAn analysis of the results indicated that product quality constitutes the foundation of marketing strategies and significantly affects purchase intention and willingness to pay through physical evidence, promotional activities, place and price. Both physical evidence and product strongly affect the purchase intention of Taiwanese consumers, followed by price, whereas price and physical evidence significantly affect willingness to pay. For Japanese consumers, price, product and promotion strongly affect purchase intention, but place and physical evidence exert negative effects; by contrast, price and promotion significantly affect willingness to pay.Originality/valueThe results determined that a modified marketing mix should be applied for agricultural products. In the domestic market, marketing should promote the modernisation and scale of pork farms and possibly the brand value or market rarity. In international markets, particularly those of neighbouring countries, marketing should focus on the promotion of Taiwanese pork as a high-quality, reasonably-priced product with transparent product information and convenient purchase channels. This study contributes to the application of marketing theory to the market for staple foods by incorporating considerations for domestic and international markets.
Firm’s openness and innovation in Industry 4.0
Authors: Capone Francesco , Innocenti Niccolò , Baldetti Filippo , Zampi Vincenzo 
Published: December 2023

Purpose
The purpose of this paper is to investigate the role of firms’ features on innovation performance in Industry 4.0, focusing on the concepts of breadth and depth of openness in the innovation process.

Design/methodology/approach
Using data gathered from 96 firms active in Industry 4.0 (I4.0) in Italy, a Poisson regression analysis is conducted to investigate the relationship between the openness of firms’ innovation processes at the level of knowledge sources and their innovation performance in I4.0.

Findings
The results highlight the relationship between the level of openness and innovative performance in I4.0. In particular, the breadth of the openness of the innovation process of enterprises is curvilinearly related to innovation in I4.0, taking an inverted U-shape.

Practical implications
Managers of firms operating in I4.0 should consider openness as a strategic response to the knowledge requirements and risks associated with the innovation process in complex technologies.

Originality/value
Through the questionnaires administered mainly to highly qualified individuals, an original and unique database has been created with information on the openness of the innovative process and the innovation performances in I4.0.
How do pandemics affect your self-preservation instinct? Disentangling the mediation effect of secondary traumatic stress
Authors: Deng Xiaohu , Fu Mengyao , Deng Shasha , Tan Chee-Wee , Jiang Zhibin 
Published: December 2023

Purpose Contemporary focus on infections and deaths in the event of pandemics may distract health institutions and medical practitioners from the psychosocial consequences of the outbreak in individuals. In light of the devastation, persistency and scarcity of pandemics, it is imperative to delve into individuals' psychological state and self-preservation instincts when confronted with the environmental danger arising from pandemic conditions and the environmental restrictions being imposed.Design/methodology/approach Guided by the self-preservation theory, the authors advance a research model to elucidate the moderated mediation effect of secondary traumatic stress on an individual's reactions when faced with environmental danger and restriction. The authors also consider the moderating influence of environmental restriction and media use diversity. The authors subsequently validated the research model via a survey with 2,016 respondents in China. The authors employed PLS-SEM to analyze the data and assess the hypothesized paths.Findings Analytical results revealed that secondary traumatic stress fully mediated the impact of environmental danger on external reliance but suppresses the mediating effects on internal reliance. The authors further confirmed that environmental restriction moderated the relationship between environmental danger and reliance. Furthermore, the authors attest to the moderating influence of media use diversity on the relationship between secondary traumatic stress and external reliance.Originality/value This study not only extends the theoretical lens of self-preservation to public health emergencies but also yields practical guidelines for coping with pandemics. Insights from this study can be harnessed to aid populations worldwide in coping and recovering from pandemics.
Enhancing prosocial behavior in the aviation industry through responsible leadership and importance of ethics and social responsibility: examining a moderated mediation model
Authors: Özkan Osman Seray , Aksoy Kürü Seval , Üzüm Burcu , Ulu Önder 
Published: December 2023

PurposeThe aim of this research, which uses the theories of social identity and social exchange, is to investigate the relationship between responsible leadership, prosocial behavior and the mediating role of psychological ownership in this relationship. In addition, the moderating role of ethical and social responsibility in the relationship between responsible leadership and psychological ownership is tested in the study.Design/methodology/approachThe sample of the research consists of 246 participants who work full-time at İstanbul Sabiha Gökçen Airport in ground handling services (GHS). The convenience sampling method was used in the research, and the research data were collected by the face-to-face survey method. The hypotheses of the research were tested with the partial least squares structural equation model (PLS-SEM) and SPSS Process Macro.FindingsAccording to the results, it was determined that responsible leadership affects prosocial behavior positively and significantly, and psychological ownership plays a mediating role in this relationship. In addition, the moderating effect of ethical and social responsibility on the relationship between responsible leadership and psychological ownership was determined. When ethical and social responsibility is perceived as high by the employees, it was revealed that the conditional indirect effect of responsible leadership on prosocial behavior through psychological ownership was strong.Research limitations/implicationsWhen responsible leadership encourages employees to take psychological ownership, they are more likely to engage in prosocial behavior. This study contributes to the field by evaluating the structures discussed with social identity and social exchange theory. In the management practice of organizations, responsible leadership should be strengthened and training should be given to develop responsible leadership.Originality/valueIn the literature review, it was observed that although there are studies conducted with responsible leadership, the concept was not examined with prosocial behavior, and it was not studied in the aviation sector, which has become indispensable for the world economy. With these features, the study distinguishes itself from others and constitutes a source of motivation for researchers.
Leave for informally admitted patients: a review of written guidance produced by mental health services in England and Wales
Authors: Ashmore Russell 
Published: December 2023

Purpose
The purpose of this paper is to report on the use and content of written guidance produced by mental health services in England and Wales describing hospital leave for informally admitted patients.

Design/methodology/approach
Guidance on leave was requested from National Health Service (NHS) mental health trusts in England and health boards in Wales (n = 61) using a Freedom of Information submission. Data were analysed using content analysis.

Findings
In total, 32 organisations had a leave policy for informal patients. Policies varied considerably in content and quality. The content of policies was not supported by research evidence. Organisations appeared to have developed their policies by either adapting or copying the guidance on section 17 leave outlined in the Mental Health Act Codes of Practice for England and Wales (Department of Health, 2016; Welsh Government, 2016). Definitions of important terms, for example, leave and hospital premises, were either absent or poorly defined. Finally, some organisations appeared to be operating pseudo-legal coercive contracts to prevent informal patients from leaving hospital wards.

Research limitations/implications
Research should be undertaken to explore the impact of local policies on the informal patient’s right to life and liberty.

Practical implications
All NHS organisations need to develop an evidence-based policy to facilitate the informal patient’s right to take leave. A set of national standards that organisations are required to comply with would help to standardise the content of leave policies.

Originality/value
To the best of the author’s knowledge, this is the first study to examine the use and content of local policies describing how informal patients can take leave from hospital.
Compensation alters estimates of the number of species required to maintain ecosystem functioning across an emersion gradient: A case study with intertidal macroalgae
Authors: Hagan James G. 
Published: December 2023

Abstract

Whether more species are required to maintain ecosystem functioning as spatial scale increases or across environmental gradients has frequently been studied by examining whether different species drive ecosystem functioning in different sites. However, this approach does rule out the counterfactual scenario where a few species could potentially maintain ecosystem functioning across sites as this requires examining which species can (or cannot) compensate for the loss of others.

Here, I used an observational study and a field‐based transplant experiment to examine the effects of species loss on biomass productivity in an intertidal marine macroalgal system. I calculated the number of species required to maintain biomass productivity across four depth zones reflecting a water emersion gradient using two commonly used observational approaches. Then, I combined hypothetical simulated extinction scenarios with field‐based transplant data of relative growth rates of all species across the four depth zones to explore how the number of species required to maintain biomass productivity across depth zones changed under counterfactual scenarios where species compensated for species loss.

The observational analyses suggested that between three and four species were required to maintain productivity across the depth zones. The simulated extinction scenarios did not. Rather, decreases in biomass productivity due to the loss of some species (e.g. Fucus spiralis, Ascophyllum nodosum) were easily compensated by other species (e.g. Fucus vesiculosus). However, for some species like F. vesiculosus, the extinction simulations suggested that compensation would be unlikely.

Commonly used observational approaches may overestimate the number of species required to maintain ecosystem functioning across environmental gradients and spatial scales.

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Joint spatial modelling of malaria incidence and vector's abundance shows heterogeneity in malaria‐vector geographical relationships
Authors: Kouame Ruth Marie A. , Edi Ako V. Constant , Cain Russell John , Weetman David , Donnelly Martin James , Sedda Luigi 
Published: December 2023

AbstractLimited attention from the modelling community has been given to ecological approaches which aim to predict geographical patterns of malaria by accounting for the joint effects of different vectors and environmental drivers.A hierarchical multivariate joint spatial Gaussian generalised linear model was developed to provide joint parameters inference and mapping of counts ofAnopheles gambiae,An. funestus,An. niliand malaria incidence collected in an area of Cote d'Ivoire. Variable‐selection methods were applied to select important predictors for each mosquito species and malaria incidence.The proposed joint model led to a general reduction of the variance in the estimates compared to independent modelling. There was high variability in the composition ofAnophelesmosquito species in the villages with each species suitability only partly overlapping geographically.Abundances ofAn. gambiae,An. funestusandAn. niliwere primarily determined by temperature. None of the species were found as a significant predictor for the others.Anopheles gambiaewas the predominant species and onlyAn. gambiaefemale abundance was an important variable (linear predictor) for malaria incidence. However, the geographic correlation analyses show that the rest ofAnophelesspecies are likely playing a role in malaria suitability.Residuals from the models of mosquito abundance and malaria cases are also correlated with each other and overlapping but in geographic patches, meaning that local drivers of vector‐malaria suitability are still present and not represented by the predictors used in the model.Synthesis and applications: Joint modelling improve predictive estimation compared to individual modelling. The accurate predictions highlighted high diversity in the association between malaria and vector species, with most of the area having more than one species suitability correlated with malaria suitability. These zones are unlikely to benefit from species‐specific interventions. Areas with correlated malaria and vector species suitability residuals contain local information, not included in the model, that requires further investigation. This will identify additional communal malaria and vectors factors that need to be considered for optimal malaria control and elimination strategies since these factors are expected to be linked to the local malaria transmission.
Relationship between physical and psychological functioning and health‐related quality of life in congenital Aniridia
Authors: Landsend Erlend Christoffer Sommer , von der Lippe Charlotte , Mediå Line , Miller Jeanette Ullmann , Berge Knut Erik , Sigurdardottir Solrun 
Published: December 2023

AbstractPurposeCongenital aniridia is a serious eye disease characterized by absence of iris to various degrees. The aims of this study were to investigate health‐related quality of life (HRQoL) in adults with aniridia and assess the relationships between HRQoL, psychological status, ocular health and obesity.MethodsTwenty‐nine adults with congenital aniridia (48% male, aged 18–79 years) participated. HRQoL was measured with SF‐36 and the EQ visual analogue scale (VAS). The physical (PCS) and mental (MCS) component summaries of the SF‐36 were calculated with higher scores indicating better HRQoL. Symptoms of anxiety and depression were measured using the Hospital Anxiety and Depression Scale (HADS). Obesity was assessed with the Patient‐Reported Outcomes in Obesity (PROS). Sociodemographic characteristics, genetic variants and ocular and medical health variables were also analysed.ResultsThe participants scored significantly lower in the general health domain of the SF‐36 than the general population (65.2 vs. 75.3, p = 0.017). The EQ VAS score was also lower in the aniridia group (64.9 vs. 77.9, p = 0.021). Low PCS score was correlated with presence of ocular pain (p = 0.019), high HADS score (p = 0.017) and high PROS score (p = 0.009). Low MCS score was related to higher educational level (p = 0.038) and high HADS score (p < 0.001). High HADS and PROS scores were both related to low EQ VAS scores.ConclusionAdults with congenital aniridia scored worse on certain measures of HRQoL than the general population. Poorer HRQoL was associated with increased symptoms of anxiety, depression and obesity and with presence of ocular pain.
Aetiology, risk factors and microbiota composition in children with prolonged diarrhoea: A prospective case–controlled cohort study
Authors: Lo Vecchio Andrea , Quitadamo Paolo , Poeta Marco , Buccigrossi Vittoria , Siani Paolo , Cioffi Valentina , Ercolini Danilo , Guarino Alfredo 
Published: December 2023

AbstractAimProlonged diarrhoea (ProD) refers to acute‐onset diarrhoea that persists for longer than 1 week. As the aetiology, risk factors and management are poorly defined, we prospectively enrolled children hospitalised in a high‐income setting to assess these outcomes and investigate the potential role of gut microbiota.MethodsAll children aged 30 days to 14 years admitted for acute‐onset diarrhoea lasting 7–14 days were included. Children consecutively admitted in the same period for acute diarrhoea (AD) served as controls. High‐throughput sequencing of 16S rRNA gene amplicons was used to analyse stool samples from a subset of patients and healthy controls.ResultsSixty‐eight with ProD and 104 with AD were enrolled. Intestinal infections were the main aetiology of diarrhoea in both groups (ProD 92.9% vs. AD 97.8%). ProD children showed a higher prevalence of bacterial infections compared to AD (30.8% vs. 8.9%, p = 0.024). Neither age, host‐related factors, nor microbiome alterations were specifically linked to ProD. However, ProD children had a more severe initial clinical presentation than AD.ConclusionProD is often the result of an unusually severe intestinal infection that runs a course longer than expected but generally resolves without further problems. No specific management or therapies should be undertaken in most cases.
Cancer cell‐derived exosomal miR‐20a‐5p inhibits CD8+ T‐cell function and confers anti‐programmed cell death 1 therapy resistance in triple‐negative breast cancer
Authors: Li Weina , Han Guohui , Li Feng , Bu Peng , Hao Yating , Huang Li , Bai Xiangdong 
Published: December 2023

AbstractCirculating miRNAs (cirmiRNAs) can be packaged into the exosomes, participating in intercellular communication, which affects the malignant progression and therapy resistance of triple‐negative breast cancer (TNBC). Currently, immune checkpoint inhibitors that regulate T‐cell function, especially antibodies against programmed cell death 1 (PD‐1) or its ligand PD‐L1, are emerging as new promising therapy for TNBC patients. However, only very limited patients showed complete or partial response to anti‐PD‐1 treatment. Dysfunction of CD8+ T cells is one of the key reasons for the immune escape of TNBC. The regulation of exosome‐derived cirmiRNAs on CD8+ T cells in TNBC deserves more investigation. Here, the cirmiR‐20a‐5p level was significantly upregulated in the plasma of TNBC patients and culture supernatant of TNBC cells. High abundance of cirmiR‐20a‐5p was correlated with a worse prognosis of TNBC. cirmiR‐20a‐5p was secreted in the form of exosomes by TNBC cells. Exosomal cirmiR‐20a‐5p was internalized into CD8+ T cells and resulted into the dysfunction of CD8+ T. A mechanism study uncovered that cirmiR‐20a‐5p targeted the nuclear protein ataxia‐telangiectasia (NPAT) and decreased NPAT expression in CD8+ T cells. An in vivo xenograft mouse model showed that cirmiR‐20a‐5p conferred TNBC to anti‐PD‐1 treatment resistance. Collectively, these findings indicated that cirmiR‐20a‐5p released by TNBC cells via exosome promotes cancer cell growth and leads to the immunosuppression by inducing CD8+ T cell dysfunction. This study suggests that targeting cirmiR‐20a‐5p might be a novel strategy for overcoming the resistance of TNBC to anti‐PD‐1 immunotherapy.
REV7 is involved in outcomes of platinum‐based chemotherapy in pancreatic cancer by controlling the DNA damage response
Authors: Tamaki Akihiro , Kato Takuya , Sakurai Yasutaka , Sato Keita , Adachi Kai , Tadehara Masayoshi , Kogami Taro , Matsushita Masahiro , Hoshino Akiyoshi , Sanoyama Itaru , Numata Yoshiko , Umezawa Atsuko , Ichinoe Masaaki , Ichihara Masatoshi , Kusano Chika , Murakumo Yoshiki 
Published: December 2023

AbstractREV7 is a multifunctional protein implicated in various biological processes, including DNA damage response. REV7 expression in human cancer cells affects their sensitivity to DNA‐damaging agents. In the present study, we investigated the significance of REV7 in pancreatic ductal adenocarcinoma (PDAC). REV7 expression was immunohistochemically examined in 92 resected PDAC specimens and 60 endoscopic ultrasound‐guided fine‐needle aspiration biopsy (EUS‐FNAB) specimens of unresectable PDAC treated with platinum‐based chemotherapy, and its association with clinicopathologic features was analyzed. Although REV7 expression was not significantly associated with the progression of primary tumors (T‐factor and Stage) in either resected or unresectable PDAC, decreased levels of REV7 expression in EUS‐FNAB specimens of unresectable PDAC were significantly associated with better outcomes of platinum‐based chemotherapy and a favorable prognosis. REV7‐deficient PDAC cell lines showed suppressed cell growth and enhanced sensitivity to cisplatin in vitro. Tumor‐bearing mice generated using REV7‐deficient PDAC cell lines also showed enhanced sensitivity to cisplatin in vivo. RNA sequencing analysis using WT and REV7‐deficient PDAC cell lines revealed that REV7 inactivation promoted the downregulation of genes involved in the DNA repair and the upregulation of genes involved in apoptosis. Our results indicate that decreased expression of REV7 is associated with better outcomes of platinum‐based chemotherapy in PDAC by suppressing the DNA damage response. It is also suggested that REV7 is a useful biomarker for predicting the outcome of platinum‐based chemotherapy and the prognosis of unresectable PDAC and is a potential target for PDAC treatment.