bluuebunny/crossref_metadata_2025_split huggingface dataset:
TurboID‐mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis
Authors: Li Xiaofang , Wei Yanping , Fei Qili , Fu Guilin , Gan Yu , Shi Chuanlin 
Published: December 2023

AbstractProximity labeling was recently developed to detect protein–protein interactions and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by streptavidin beads, and identified by mass spectrometry (MS). TurboID is an engineered biotin ligase with high catalytic efficiency, which is used for proximity labeling. Although TurboID‐based proximity labeling technology has been successfully established in mammals, its application in plant systems is limited. Here, we report the usage of TurboID for proximity labeling of FIP37, a core member of m6A methyltransferase complex, to identify FIP37 interacting proteins in Arabidopsis thaliana. By analyzing the MS data, we found 214 proteins biotinylated by GFP‐TurboID‐FIP37 fusion, including five components of m6A methyltransferase complex that have been previously confirmed. Therefore, the identified proteins may include potential proteins directly involved in the m6A pathway or functionally related to m6A‐coupled mRNA processing due to spatial proximity. Moreover, we demonstrated the feasibility of proximity labeling technology in plant epitranscriptomics study, thereby expanding the application of this technology to more subjects of plant research.
Breast cancer screening motivation and behaviours of women aged over 75 years
Authors: Dickson‐Swift Virginia , Adams Joanne , Spelten Evelien , Blackberry Irene , Wilson Carlene , Yuen Eva 
Published: December 2023

AbstractObjectiveIn Australia, breast screening is offered free every two years to women aged 50–74 years. Women aged ≥75 are eligible to receive a free mammogram but do not receive an invitation. This study aimed to explore the motivations and behaviours of women living in Australia aged ≥75 years regarding ongoing breast cancer screening given the public health guidance.MethodsSixty women aged ≥75 were recruited from metropolitan, regional, and rural areas across Australia to participate in a descriptive qualitative study. Semi‐structured interviews were used to seek reflection on women's experience of screening, any advice they had received about screening beyond 75, their understanding of the value of screening and their intention to participate in the future. Thematic analysis of transcripts led to the development of themes.ResultsThemes resulting from the study included: reasons to continue and discontinue screening, importance of inclusivity in the health system and availability of information. Regular screeners overwhelmingly wished to continue screening and had strong beliefs in the benefits of screening. Women received limited information about the benefits or harms of screening beyond age 75 and very few had discussed screening with their Primary Healthcare Provider. No longer receiving an invitation to attend screening impacted many women's decision‐making.ConclusionMore information via structured discussion with health professionals is required to inform women about the risks and benefits of ongoing screening. No longer being invited to attend screening left many women feeling confused and for some this led to feelings of discrimination.
Lyotropic Liquid Crystals of Colloidal Gibbsite Nanoplatelets: Phase Transition, Kinetic Characterization, and Confinement Effect
Authors: Trung Le Gia , Gwag Jin Seog , Kang Shin‐Woong 
Published: December 2023

Abstract2D gibbsite nanoplatelets, [γ‐Al(OH)3], are widely used as an inorganic mineral platform for 2D lyotropic liquid crystal (LC) colloids. These particles are synthesized and enlarged using an improved hydrolysis method, resulting in highly crystalline (96.5%), low polydispersity (15.1%), and readily dispersible colloids in water. The aqueous mesomorphic system is characterized for the isotropic‐to‐nematic phase transition by analyzing number density and shear viscosity. Thermal stability is assessed through thermogravimetric analysis. Additionally, kinetic and thermodynamic parameters for 2D gibbsite nanoparticles are determined for the first time using three models (Coats‐Redfern, Friedman, and Kissinger). In particular, the activation and Gibbs free energies for the first dehydration stage of gibbsite yield ranges of 98‒128 kJ mol−1 and 135‒161 kJ mol−1, respectively. To investigate the confinement effect of colloidal gibbsite‐LCs, an isotropic gibbsite dispersion is introduced into a tube, leading to the uniform formation of gibbsite‐LC layers along two distinct pathways: tangential to the liquid‐air interface and as concentric circles along the tube walls. These findings offer valuable insights into potential applications, particularly in the domain of gas barrier inorganic films across various specialized fields.
Enhancing Zn2+ Storage Performance by Constructing the Interfaces Between VO2 and Co–N–C Layers
Authors: Yuan Guo‐Qiang , Wei Xing , Su Yi‐Chun , Zhou Tian‐Yu , Hu Jin‐Liang , An Yang , Zhou Song‐Lin , Zhao Wen‐Qiang , Xia Jun , Liu Yang‐Yi 
Published: December 2023

AbstractVanadium oxides have aroused attention as cathode materials in aqueous zinc‐ion batteries (AZIBs) due to their low cost and high safety. However, low ion diffusion and vanadium dissolution often lead to capacity decay and deteriorating stability during cycling. Herein, vanadium dioxides (VO2) nanobelts are coated with a single‐atom cobalt dispersed N‐doped carbon (Co–N–C) layer via a facile calcination strategy to form Co–N–C layer coated VO2 nanobelts (VO2@Co–N–C NBs) for cathodes in AZIBs. Various in‐/ex situ characterizations demonstrate the interfaces between VO2 layers and Co–N–C layers can protect the VO2 NBs from collapsing, increase ion diffusion, and enhance the Zn2+ storage performance. Additional density functional theory (DFT) simulations demonstrate that Co─O─V bonds between VO2 and Co–N–C layers can enhance interfacial Zn2+ storage. Moreover, the VO2@Co–N–C NBs provided an ultrahigh capacity (418.7 mAh g−1 at 1 A g−1), outstanding long‐term stability (over 8000 cycles at 20 A g−1), and superior rate performance.
Laparoscopic but not open surgical skills can be transferred to robot‐assisted surgery: A systematic review and meta‐analysis
Authors: Schmidt Mona W. , Fan Carolyn , Köppinger Karl F. , Schmidt Leon P. , Brechter Anna , Limen Eldrige F. , Vey Johannes A. , Metz Matthes , Müller‐Stich Beat P. , Nickel Felix , Kowalewski Karl‐Friedrich 
Published: December 2023

AbstractBackgroundWith an increase in robot‐assisted surgery across all specialties, adequate training and credentialing strategies need to be identified to ensure patients safety. The meta‐analysis assesses the transferability of technical surgical skills between laparoscopic surgery, open surgery, and robot‐assisted surgery.DesignA systematic search was conducted in Medline, Cochrane Central Register of Controlled Trials, and Web of Science. Outcomes were categorized into time, process, product, and composite outcome measures and pooled separately using Hedges'g (standardized mean difference [SMD]). Subgroup analyses were performed to assess the effect of study design, virtual reality platforms and task difficulty.ResultsOut of 14,120 screened studies, 30 were included in the qualitative synthesis and 26 in the quantitative synthesis. Technical surgical skill transfer was demonstrated from laparoscopic to robot‐assisted surgery (composite: SMD 0.40, 95%‐confidence interval [CI] [0.19; 0.62], time: SMD 0.62, CI [0.33; 0.91]) and vice versa (composite: SMD 0.66, CI [0.33; 0.99], time [basic skills]: SMD 0.36, CI [0.01; 0.72]). No skill transfer was seen from open to robot‐assisted surgery with limited available data.ConclusionTechnical surgical skills can be transferred from laparoscopic to robot‐assisted surgery and vice versa. Robot‐assisted and laparoscopic surgical skills training and credentialing should not be regarded separately, but a reasonable combination could shorten overall training times and increase efficiency. Previous experience in open surgery should not be considered as an imperative prerequisite for training in robot‐assisted surgery. Recommendations for studies assessing skill transfer are proposed to increase comparability and significance of future studies.PROSPERO Registration NumberPROSPERO CRD42018104507.
Long term survival outcomes of surgery combined with hyperthermic intraperitoneal chemotherapy for perforated low‐grade appendiceal mucinous neoplasms: A multicenter retrospective study
Authors: Han Zhenlong , Liu Xiaodong , Tian Yulong , Shen Shuai , Wang Hongbo , Hu Shuiqing , Wu Xudong , Yan Zhao , Lu Cunlong , Wang Peixin , Bai Yingcheng , Kong Ying , Wang Liankai , Cao Shougen , Li Zequn , Zhong Hao , Meng Cheng , Zhou Yanbing 
Published: December 2023

AbstractBackgroundLow‐grade appendiceal mucinous neoplasms (LAMN) are very rare, accounting for approximately 0.2%–0.5% of gastrointestinal tumors. We conducted a multicenter retrospective study to explore the impact of different surgical procedures combined with HIPEC on the short‐term outcomes and long‐term survival of patients.MethodsWe retrospectively analyzed the clinicopathological data of 91 LAMN perforation patients from 9 teaching hospitals over a 10‐year period, and divided them into HIPEC group and non‐HIPEC group based on whether or not underwent HIPEC.ResultsOf the 91 patients with LAMN, 52 were in the HIPEC group and 39 in the non‐HIPEC group. The Kaplan‐Meier method predicted that 52 patients in the HIPEC group had 5‐ and 10‐year overall survival rates of 82.7% and 76.9%, respectively, compared with predicted survival rates of 51.3% and 46.2% for the 39 patients in the non‐HIPEC group, with a statistically significant difference between the two groups (χ2 = 10.622, p = 0.001; χ2 = 10.995, p = 0.001). Compared to the 5‐year and 10‐year relapse‐free survival rates of 75.0% and 65.4% in the HIPEC group, respectively, the 5‐year and 10‐year relapse‐free survival rates of 48.7% and 46.2% in the non‐HIPEC group were significant different between the two outcomes (χ2 = 8.063, p = 0.005; χ2 = 6.775, p = 0.009). The incidence of postoperative electrolyte disturbances and hypoalbuminemia was significantly higher in the HIPEC group than in the non‐HIPEC group (p = 0.023; p = 0.044).ConclusionsThis study shows that surgery combined with HIPEC can significantly improve 5‐year and 10‐year overall survival rates and relapse‐free survival rates of LAMN perforation patients, without affecting their short‐term clinical outcomes.
Monitoring urban heat island intensity based on GNSS tomography technique
Authors: Xia Pengfei , Peng Wei , Yuan Peng , Ye Shirong 
Published: December 2023

AbstractMonitoring urban heat island (UHI) effect is critical because it causes health problems and excessive energy consumption more energy when cooling buildings. In this study, we propose an approach for UHI monitoring by fusing data from ground-based global navigation satellite system (GNSS), space-based GNSS radio occultation (RO), and radiosonde. The idea of the approach is as follows: First, the first and second grid tops are defined based on historical RO and radiosonde observations. Next, the wet refractivities between the first and second grid tops are fitted to higher-order spherical harmonics and they are used as the inputs of GNSS tomography. Then, the temperature and water vapor partial pressure are estimated by using best search method based on the tomography-derived wet refractivity. In the end, the UHI intensity is evaluated by calculating the temperature difference between the urban regions and nearby rural regions. Feasibility of the UHI intensity monitoring approach was evaluated with GNSS RO and radiosonde data in 2010–2019, as well as ground-based GNSS data in 2020 in Hong Kong, China, by taking synoptic temperature data as reference. The result shows that the proposed approach achieved an accuracy of 1.2 K at a 95% confidence level.
Geometric Methods for Adjoint Systems
Authors: Tran Brian Kha , Leok Melvin 
Published: December 2023

AbstractAdjoint systems are widely used to inform control, optimization, and design in systems described by ordinary differential equations or differential-algebraic equations. In this paper, we explore the geometric properties and develop methods for such adjoint systems. In particular, we utilize symplectic and presymplectic geometry to investigate the properties of adjoint systems associated with ordinary differential equations and differential-algebraic equations, respectively. We show that the adjoint variational quadratic conservation laws, which are key to adjoint sensitivity analysis, arise from (pre)symplecticity of such adjoint systems. We discuss various additional geometric properties of adjoint systems, such as symmetries and variational characterizations. For adjoint systems associated with a differential-algebraic equation, we relate the index of the differential-algebraic equation to the presymplectic constraint algorithm of Gotay et al. (J Math Phys 19(11):2388–2399, 1978). As an application of this geometric framework, we discuss how the adjoint variational quadratic conservation laws can be used to compute sensitivities of terminal or running cost functions. Furthermore, we develop structure-preserving numerical methods for such systems using Galerkin Hamiltonian variational integrators (Leok and Zhang in IMA J. Numer. Anal. 31(4):1497–1532, 2011) which admit discrete analogues of these quadratic conservation laws. We additionally show that such methods are natural, in the sense that reduction, forming the adjoint system, and discretization all commute, for suitable choices of these processes. We utilize this naturality to derive a variational error analysis result for the presymplectic variational integrator that we use to discretize the adjoint DAE system. Finally, we discuss the application of adjoint systems in the context of optimal control problems, where we prove a similar naturality result.
Decadal prediction of Northeast Asian winter precipitation with CMIP6 models
Authors: Xin Xiaoge , Wu Tongwen , Zheng Mengzhe , Fang Yongjie , Lu Yixiong , Zhang Jie 
Published: December 2023

AbstractThis study evaluates the decadal prediction skill of 13 forecast systems in predicting winter precipitation over Eurasia, contributing to the Decadal Climate Prediction Project of the Coupled Model Intercomparison Project Phase 6. Northeast Asia stands out as a region with improved decadal prediction skill for forecast years 2–5 due to the initialization. Observations show anticyclonic and cyclonic wind anomalies over the North Pacific and Northeast Asia, respectively, with southwesterly flow to the east of Northeast Asia. Ten forecast systems reproduce such circulation anomalies favoring abundant winter precipitation in Northeast Asia. The significant positive (negative) correlations between the detrended Northeast Asian precipitation (NEAP) and AMV (PDO-like) time series are reproduced by seven (nine) forecast systems. However, most forecast systems underestimate the correlation between the NEAP and the AMV, and have relatively low skill in predicting the PDO. Further improvements in these aspects will help to improve the decadal prediction skill of winter precipitation over Northeast Asia. The multi-model ensemble (MME) is able to reproduce both links of NEAP with AMV and PDO-like variability. The MME demonstrates significant skill and outperforms the individual forecast systems in predicting the NEAP for all 4-year averaged periods in the range of 1–8 years, demonstrating the benefits of using the ensemble mean of multiple models.
Apoptosis and its pathways as targets for intracellular pathogens to persist in cells
Authors: Rodríguez-González Jorge , Gutiérrez-Kobeh Laila 
Published: December 2023

AbstractApoptosis is a finely programmed process of cell death in which cells silently dismantle and actively participate in several operations such as immune response, differentiation, and cell growth. It can be initiated by three main pathways: the extrinsic, the perforin granzyme, and the intrinsic that culminate in the activation of several proteins in charge of tearing down the cell. On the other hand, apoptosis represents an ordeal for pathogens that live inside cells and maintain a strong dependency with them; thus, they have evolved multiple strategies to manipulate host cell apoptosis on their behalf. It has been widely documented that diverse intracellular bacteria, fungi, and parasites can interfere with most steps of the host cell apoptotic machinery to inhibit or induce apoptosis. Indeed, the inhibition of apoptosis is considered a virulence property shared by many intracellular pathogens to ensure productive replication. Some pathogens intervene at an early stage by interfering with the sensing of extracellular signals or transduction pathways. Others sense cellular stress or target the apoptosis regulator proteins of the Bcl-2 family or caspases. In many cases, the exact molecular mechanisms leading to the interference with the host cell apoptotic cascade are still unknown. However, intense research has been conducted to elucidate the strategies employed by intracellular pathogens to modulate host cell death. In this review, we summarize the main routes of activation of apoptosis and present several processes used by different bacteria, fungi, and parasites to modulate the apoptosis of their host cells.
When is a Planar Rod Configuration Infinitesimally Rigid?
Authors: Lundqvist Signe , Stokes Klara , Öhman Lars-Daniel 
Published: December 2023

AbstractWe investigate the rigidity properties of rod configurations. Rod configurations are realizations of rank two incidence geometries as points (joints) and straight lines (rods) in the Euclidean plane, such that the lines move as rigid bodies, connected at the points. Note that not all incidence geometries have such realizations. We show that under the assumptions that the rod configuration exists and is sufficiently generic, its infinitesimal rigidity is equivalent to the infinitesimal rigidity of generic frameworks of the graph defined by replacing each rod by a cone over its point set. To put this into context, the molecular conjecture states that the infinitesimal rigidity of rod configurations realizing 2-regular hypergraphs is determined by the rigidity of generic body and hinge frameworks realizing the same hypergraph. This conjecture was proven by Jackson and Jordán in the plane, and by Katoh and Tanigawa in arbitrary dimension. Whiteley proved a version of the molecular conjecture for hypergraphs of arbitrary degree that have realizations as independent body and joint frameworks. Our result extends his result to hypergraphs that do not necessarily have realizations as independent body and joint frameworks, under the assumptions listed above.
Management of radiation-induced oral mucositis in head and neck cancer patients: a real-life survey among 25 Italian radiation oncology centers
Authors: Bergamaschi Luca , Vincini Maria Giulia , Zaffaroni Mattia , Pepa Matteo , Angelicone Ilaria , Astone Antonio , Bergamini Cristiana , Buonopane Sergio , Conte Mario , De Rosa Nicola , Deantoni Chiara , Dell’Oca Italo , Di Gennaro Davide , Di Muzio Nadia , Osti Mattia Falchetto , Federico Manuela , Ferini Gianluca , Franzese Ciro , Gatti Marco , Grillo Antonietta , Iorio Vincenzo , Manzo Roberto , Marmiroli Luca , Martin Giuseppe , Mazzuca Federica , Molinaro Maria Angela , Muto Matteo , Pacelli Roberto , Pepe Alfonsina , Perillo Annarita , Russo Donatella , Salerno Francesca , Spadaro Pietro , Viola Anna , Iorio Giuseppe Carlo , Muto Paolo , Ricardi Umberto , Alterio Daniela 
Published: December 2023

Abstract
Aim
Radiation-induced oral mucositis (RIOM) is the most frequent side effect in head and neck cancer (HNC) patients treated with curative radiotherapy (RT). A standardized strategy for preventing and treating RIOM has not been defined. Aim of this study was to perform a real-life survey on RIOM management among Italian RT centers.

Methods
A 40-question survey was administered to 25 radiation oncologists working in 25 different RT centers across Italy.

Results
A total of 1554 HNC patients have been treated in the participating centers in 2021, the majority (median across the centers 91%) with curative intent. Median treatment time was 41 days, with a mean percentage of interruption due to toxicity of 14.5%. Eighty percent of responders provide written oral cavity hygiene recommendations. Regarding RIOM prevention, sodium bicarbonate mouthwashes, oral mucosa barrier agents, and hyaluronic acid-based mouthwashes were the most frequent topic agents used. Regarding RIOM treatment, 14 (56%) centers relied on literature evidence, while internal guidelines were available in 13 centers (44%). Grade (G)1 mucositis is mostly treated with sodium bicarbonate mouthwashes, oral mucosa barrier agents, and steroids, while hyaluronic acid-based agents, local anesthetics, and benzydamine were the most used in mucositis G2/G3. Steroids, painkillers, and anti-inflammatory drugs were the most frequent systemic agents used independently from the RIOM severity.

Conclusion
Great variety of strategies exist among Italian centers in RIOM management for HNC patients. Whether different strategies could impact patients’ compliance and overall treatment time of the radiation course is still unclear and needs further investigation.
Hölder regularity for parabolic fractional p-Laplacian
Authors: Liao Naian 
Published: December 2023

AbstractLocal Hölder regularity is established for certain weak solutions to a class of parabolic fractional p-Laplace equations with merely measurable kernels. The proof uses DeGiorgi’s iteration and refines DiBenedetto’s intrinsic scaling method. The control of a nonlocal integral of solutions in the reduction of oscillation plays a crucial role and entails delicate analysis in this intrinsic scaling scenario. Dispensing with any logarithmic estimate and any comparison principle, the proof is new even for the linear case.
Behavior of a Rock Mass in Uplift Field Tests of Rock Anchors
Authors: Grindheim Bjarte , Li Charlie C. , Høien Are Håvard , Lia Leif 
Published: December 2023

AbstractRock anchors are high-capacity reinforcement measures used to stabilize large-scale infrastructures. According to the literature, they can fail in one of the four ways: (1) steel tensile failure; (2) tendon–grout interface failure; (3) grout–rock interface failure; and (4) rock mass uplift failure. Rock mass uplift field tests were performed in a limestone quarry to test this fourth failure mode. The tests were designed to induce rock mass failure around rock anchors. The ground surface heaved asymmetrically around the anchors and the pre-existing joints in the rock mass tended to open first. The seismic activity in the rock mass was greatest close to the anchor, decreasing with distance. The failure crater in the rock followed the joint sets in the rock mass, and the shape was partly controlled by the pre-existing joints. The apex angle of the failures was measured to be between 125 and 140$$^{\circ }$$

∘

. The horizontal stress in the rock mass increased when the anchor was pulled, indicating load arching around the anchor. The observed failure was a combination of rock mass uplift and grout–rock bond failure. The measured anchor capacity was much higher than the estimated rock mass capacity using the recommended design methods of the Norwegian Public Roads Administration.