bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ih current stabilizes excitability in rodent DRG neurons and reverses hyperexcitability in a nociceptive neuron model of inherited neuropathic pain
Authors: Vasylyev Dmytro V. , Liu Shujun , Waxman Stephen G. 
Published: October 2023

AbstractWe show here that hyperpolarization‐activated current (Ih) unexpectedly acts to inhibit the activity of dorsal root ganglion (DRG) neurons expressing WT Nav1.7, the largest inward current and primary driver of DRG neuronal firing, and hyperexcitable DRG neurons expressing a gain‐of‐function Nav1.7 mutation that causes inherited erythromelalgia (IEM), a human genetic model of neuropathic pain. In this study we created a kinetic model of Ih and used it, in combination with dynamic‐clamp, to study Ih function in DRG neurons. We show, for the first time, that Ih increases rheobase and reduces the firing probability in small DRG neurons, and demonstrate that the amplitude of subthreshold oscillations is reduced by Ih. Our results show that Ih, due to slow gating, is not deactivated during action potentials (APs) and has a striking damping action, which reverses from depolarizing to hyperpolarizing, close to the threshold for AP generation. Moreover, we show that Ih reverses the hyperexcitability of DRG neurons expressing a gain‐of‐function Nav1.7 mutation that causes IEM. In the aggregate, our results show that Ih unexpectedly has strikingly different effects in DRG neurons as compared to previously‐ and well‐studied cardiac cells. Within DRG neurons where Nav1.7 is present, Ih reduces depolarizing sodium current inflow due to enhancement of Nav1.7 channel fast inactivation and creates additional damping action by reversal of Ih direction from depolarizing to hyperpolarizing close to the threshold for AP generation. These actions of Ih limit the firing of DRG neurons expressing WT Nav1.7 and reverse the hyperexcitability of DRG neurons expressing a gain‐of‐function Nav1.7 mutation that causes IEM.
imageKey points
Hyperpolarization‐activated cyclic nucleotide‐gated (HCN) channels, the molecular determinants of hyperpolarization‐activated current (Ih) have been characterized as a ‘pain pacemaker’, and thus considered to be a potential molecular target for pain therapeutics.
Dorsal root ganglion (DRG) neurons express Nav1.7, a channel that is not present in central neurons or cardiac tissue. Gain‐of‐function mutations (GOF) of Nav1.7 identified in inherited erythromelalgia (IEM), a human genetic model of neuropathic pain, produce DRG neuron hyperexcitability, which in turn produces severe pain.
We found that Ih increases rheobase and reduces firing probability in small DRG neurons expressing WT Nav1.7, and demonstrate that the amplitude of subthreshold oscillations is reduced by Ih.
We also demonstrate that Ih reverses the hyperexcitability of DRG neurons expressing a GOF Nav1.7 mutation (L858H) that causes IEM.
Our results show that, in contrast to cardiac cells and CNS neurons, Ih acts to stabilize DRG neuron excitability and prevents excessive firing.
Analysis on Effects of Material Parameters on Thermoelastic Instability of Separate Plate in Wet Clutch
Authors: Li Jiaxue , Jin Wenrui , Zhang Zhigang , Lv Xiaoxiao , Zhang Tao 
Published: October 2023

Abstract
The working characteristics of wet clutches have an important impact on the safety performance of vehicles. In order to obtain the thermoelastic instability characteristics of wet clutch separate plate, a finite element modeling method is proposed. The temperature field calculation model of separate plate and its thermo-hydro-mechanical coupling relationship are constructed. The distribution law of high-temperature hot spots on the surface of separate plate is obtained and the thermoelastic instability mechanism is revealed. Effectiveness of the simulation model is verified by road test, and surface topography of separate plate is observed by scanning electron microscope. A thermoelastic instability calculation model considering different material parameters is established. The temperature field distribution law is reviewed under different elastic moduli, specific heat capacities, thermal conductivities, and thermal expansion coefficients. Results show that increasing the specific heat capacity and thermal conductivity of the separate plate, decreasing the elastic modulus and thermal expansion coefficient can improve the stability of the system. The thermal conductivity and thermal expansion coefficient have important effects on the thermoelastic instability. The specific heat capacity has a certain effect, and the elastic modulus’ effect is the least. The research results of this paper can provide theoretical support for optimizing the structure of wet clutch and improving the stability of the system.
Near-Critical CO2-Assisted Liquefaction-Extraction of Biomass and Wastes to Fuels and Value-Added Products
Authors: Burra Kiran Raj Goud , Sahin Murat , Zheng Ying , Gupta Ashwani K. 
Published: October 2023

Abstract
With the growing need for sustainable carbon-neutral liquid fuels, low-grade feedstocks, such as lignocellulosic biomass, and municipal solid wastes offer sufficient potential via thermochemical conversion. But the existing thermochemical means are limited in feed flexibility and scalability and require significant processing (energy and costs) of the intermediates. Bio-oil/biocrude intermediate from fast pyrolysis and hydrothermal techniques is impeded by issues of stability and oxygen content, along with hydrotreating viability. To address these issues, we investigated a novel pathway of near-critical CO2-assisted integrated liquefaction-extraction (NILE) technology in conceptual aspects for conversion of various biomass and municipal solid wastes into high-quality biocrude with high compatibility for co-hydrotreating with traditional fossil crude for liquid fuel needs in power and transportation sectors. Using supercritical CO2 for dewatering wet feedstocks, for liquefaction, and extraction for lighter biocrude has produced biocrude with lower oxygen content by 50%, lowered metal content by 90%, stable viscosity, low acidity, and good aging stability compared to that produced from hydrothermal liquefaction along with higher hydrotreating and co-hydrotreating compatibility. Hydrotreating of the biocrude extract from supercritical CO2 extraction also was feasible with no detected coke deposition, an oxygen content of 1%, and catalyst deactivation. The validation and capabilities of the NILE concept urge for its further development to obtain sustainable liquid fuels with lower greenhouse gas emissions and costs.
Complete genome sequences of eight
Auritidibacter ignavus
strains isolated from ear infections in Germany
Authors: Bähr Tobias , Baumhögger Ariane , Geis Gabriele , Gatermann Sören 
Published: October 2023

ABSTRACT

Here, we present the complete genome sequences of eight
Auritidibacter ignavus
strains isolated from clinical samples of patients with ear infections in Bochum, Germany. The sequence information will give assistance to greater knowledge about the virulence potential of this unfamiliar putative pathogen.
Shelf-Life Feasibility Study of Moringa oleifera Seasoned Beef
Authors: Ayirezang Florence Adwoa , Arhin Reuben Essel , Whyte Barry Kojo , Antwi Emmanuel Opoku , Boateng Belinda Nana Ama 
Published: October 2023

This study explored the stability of beef treated with Moringa oleifera ethanolic leaves extract (MELE) stored at 4°C for 10 days. Beef samples were treated with MELE concentrations of 8%, 4%, and 2%. A 3% NaCl-treated beef sample and control were included. Samples were taken every 48 hours for microbiological analysis. The Nordic Committee on Food Analysis methods were used to determine the mesophilic, coliform, Staphylococcus aureus, Escherichia coli, and Salmonella sp. enumeration. The organoleptic properties were tested using the 5-point scale hedonism. There was a significant difference (

p

< 0.05) in the mean mesophilic and coliform counts, which varied from 2.0 × 103 ± 0.0 to 8.9 × 107 ± 0.4 CFU/ml and 1.0 × 101 ± 0.1 to 8.9 × 104 ± 0.3 CFU/ml, respectively, from storage day two to ten when beef treated with MELE was compared to the 3% NaCl and the control. Staphylococcus aureus grew on all the beef samples across all the different incubation periods, but a dose-dependent antibacterial activity of the M. oleifera-treated beef was observed for S. aureus with the maximum inhibition recorded for 8% MELE. Beef seasoned with MELE was free of E. coli throughout storage. Salmonella sp. was not detected in all the beef samples. Microbial loads increased with decreasing concentrations of MELE. The overall acceptability of MELE-treated beef decreased with increasing concentration of MELE treatment. MELE may find application in food preservation. The probability of synergistic activity of salt and M. oleifera leaves extract can be explored in further studies.
CACNA1D
Gene Polymorphisms Associate With Increased Blood Pressure and Salt Sensitivity of Blood Pressure in White Individuals
Authors: Stanton Ana Maria , Heydarpour Mahyar , Williams Jonathan S. , Williams Gordon H. , Adler Gail K. 
Published: October 2023

BACKGROUND:

Disease-causing mutations in
CACNA1D
gene occur in aldosterone-producing adenomas and familial hyperaldosteronism. We determined whether single nucleotide polymorphisms in
CACNA1D
gene associate with higher aldosterone resulting in salt sensitivity of blood pressure (BP) and increased BP in men and women.

METHODS:

Data were obtained from the HyperPATH (International Hypertension Pathotypes) cohort, where participants completed a cross-over intervention of liberal and restricted sodium diets. Multi-Ethnic Genotyping Array identified 104
CACNA1D
single nucleotide polymorphisms that met quality control. Single nucleotide polymorphism is rs7612148 strongly associated with systolic BP and was selected for study in 521 White participants in 3 scenarios ([1] hypertensives; [2] normotensives; [3] total population=hypertensives+normotensives) using multivariate regression analysis.

RESULTS:
In the total population and hypertensives, but not normotensives, risk allele carriers (CC, GC), as compared with nonrisk allele homozygotes (GG), exhibited higher salt sensitivity of BP and, on liberal sodium diet, higher systolic BP, lower baseline and angiotensin II–stimulated aldosterone, and lower plasma renin activity. On restricted sodium diet, BP was similar across genotypes, suggesting sodium restriction corrected/neutralized the genotype effect on BP. Because increased aldosterone did not seem to drive the increased salt sensitivity of BP and increased BP on liberal sodium diet, we assessed renal plasma flow. Renal plasma flow increase from restricted to liberal sodium diets was blunted in risk allele homozygotes in the total population and in hypertensives. A replication study in another cohort HyperPATH B (International Hypertension Pathotypes Cohort B) confirmed BP-genotype associations.

CONCLUSIONS:

CACNA1D
rs7612148 risk allele associated with increased BP and salt sensitivity of BP, likely due to an impaired ability to increase renal plasma flow in response to a liberal sodium diet and not to excess aldosterone.
Development First! China’s Policy Transfer to Africa
Authors: Lammich Georg 
Published: October 2023

Abstract
China has emerged as an important norm-shaper in Africa in recent years and can be credited with influencing various policy sectors across the whole continent. Despite the massive impact COVID-19 had on China’s presence in Africa and the palpable decrease in financial and technical cooperation in recent years, in the field of policy dissemination China has been and still is one of the most influential external actors on the continent. Numerous ideas and practices from China have found their way into African politics, and China has directly or indirectly (and intentionally or otherwise) affected how Africans see development and the shape and implementation of development practices. This article traces the rise of China as a norm-provider to African elites and compares the impact of two influential policy sets – the development-security nexus and the Belt and Road Initiative – on African policymaking. It analyzes why some policies and ideas travelling from China have reverberated with African leaders and have had a direct impact on the developmental agenda of several countries while other concepts did not take root on the continent.
The Obliteration of Publisher Identity
Authors: Gerčar Jaka 
Published: October 2023

Abstract
The title of this paper is taken from a chapter in Roberto Calasso’s The Art of the Publisher, in which the Italian publisher and writer notes a historical shift in contemporary publishing away from publishing as ‘form’, supposedly a publishing innovation of the first decade of the 20th century. Since Calasso never elaborated on his notion of publishing as form, this paper attempts to sketch the most important facets of this notion, embed it in the context of publishing theory, and suggest that it bears considerable similarities to auteur theory in cinema. After identifying three layers of publishing form, the author analyses how recent developments in contemporary publishing might relate to Calasso’s concern about the blurring of distinctions between publishers.
The Spiritual Teachings of Zakariyyā Kāndhlavī (d. 1982) in Fażāʾil-i Durūd Sharīf
Authors: Jafar Imad 
Published: October 2023

Abstract
Although the influential transnational Deobandi missionary organisation Tablīghī Jamāʿat is scarcely portrayed in contemporary popular media as a “Sufi movement,” the elders of the trend – such as the late Zakariyyā Kāndhlavī (d. 1402/1982) – were staunch Sufi shaykhs. Therefore, it is not surprising to find that one of the core texts of the Tablīghī Jamāʿat – Kāndhlavī’s Fażāʾil-i ʿAmāl – is full of many distinctly Sufi concepts. In the following essay, I look into the spiritual themes found in that particular volume of Kāndhlavī’s aforementioned work which deals specifically with the virtuous act of sending blessings upon the Prophet. In doing so, we will see that the Tablīghī Jamāʿat – deeply rooted in Sufi thought as it is – is very far removed from the Wahhabism with which it is occasionally associated in popular Western imagination.
Efficacy of telemedicine-based antimicrobial stewardship program to combat antimicrobial resistance: A systematic review and meta-analysis
Authors: Dirjayanto Valerie J , Lazarus Gilbert , Geraldine Priscilla , Dyson Nathaniel G , Triastari Stella K , Anjani Jasmine V , Wisnu Nayla KP , Sugiharta Adrianus J 
Published: October 2023

Introduction Antimicrobial resistance (AMR) is a major public health threat. Improving antimicrobial use is the main strategy against AMR, but it is challenging to implement especially in low-resource settings. Thus, this review aims to explore the efficacy of telehealth-based antimicrobial stewardship programs (ASP), which is more accessible. Methods Registered to PROSPERO and following PRISMA guidelines, literature search was performed in databases including PubMed, Scopus, Cochrane, Science Direct, EBSCOhost, EMBASE, and Google Scholar, searching for studies implementing telehealth ASP. Critical appraisal of studies was performed using Newcastle-Ottawa Scale for Cohort Studies (NOS), Cochrane Risk-of-Bias tool (RoB) 2.0, and Risk Of Bias In Non-randomised Studies-of Interventions (ROBINS-I). We utilized inverse variance, random effects model to obtain the pooled odds ratio (OR) and mean difference (MD) estimates, as well as sensitivity and subgroup analysis. Results and Discussion The search yielded 14 studies. Telehealth-based ASP was associated with better adherence to guidelines (pooled OR: 2.78 [95%CI:1.29–5.99], p = 0.009; I2 = 93%), within which streamlining yielded better odds (pooled OR: 30.54 [95%CI:10.42–89.52], p < 0.001) more than the compliance with policy subgroup (pooled OR: 1.60 [95%CI:1.02–2.51], p = 0.04). The odds of antimicrobial prescription rate reduced significantly (pooled OR: 0.60 [95%CI:0.42–0.85], p = 0.005; I2 = 94%), especially for the lower respiratory infection subgroup (pooled OR: 0.37 [95%CI:0.28–0.49], p < 0.001). Days of therapy decreased (pooled MD: -47.12 [95%CI: −85.78– −8.46], p = 0.02; I2 = 100%), with the greatest effect in acute care settings (pooled MD: -97.73 [95%CI:−147.48–47.97], p = 0.0001). Mortality did not change significantly (pooled OR: 1.20 [95%CI:0.69–2.10], p = 0.52; I2 = 63%). Conclusion Telehealth-based ASP was proven beneficial to increase adherence to guideline and reduce prescription rates, without significantly affecting patient clinical outcome. After further studies, we recommend more widespread use of telemedicine to combat AMR.
Cir-DNA Sequencing Revealed the Landscape of Extrachromosomal Circular DNA in Articular Cartilage and the Potential Roles in Osteoarthritis
Authors: Xiang Wei , Zhang Tongyi , Li Song , Gong Yunquan , Luo Xiaoqing , Yuan Jing , Wu Yaran , Yan Xiaojing , Xiong Yan , Lian Jiqin , Zhao Guangyu , Gao Changyue , Kuang Liang , Ni Zhenhong 
Published: October 2023

Objective Extrachromosomal circular DNA (eccDNA) has been shown to be involved in several physiological and pathological processes including immunity, inflammation, aging, and tumor. However, the expression of eccDNA in cartilage has not been reported until now. In this study, we aimed to investigate the landscape of eccDNA in articular cartilage and analyze the potential roles in osteoarthritis (OA). Methods The samples of articular cartilage were obtained from total knee arthroplasty (TKA) donors with OA. The mitochondrial DNA (mtDNAs) and the linear DNAs from chondrocytes of articular cartilage were removed. Then the eccDNAs were enriched for cir-DNA sequencing. After quality control evaluation, we systematically revealed the identified eccDNA data including size distribution, the size range, and sequence pattern. Moreover, we explored and discussed the potential roles of eccDNA in OA via motif analysis and Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results The chondrocytes from OA cartilage contained an abundance of eccDNAs, which was termed as OC-eccDNAs (OA cartilage-derived eccDNA). The characteristics of OC-eccDNAs were tissue-specific, including the distribution, the size range, and sequence pattern. Moreover, the functional analysis indicated that eccDNA may be involved in the homeostasis maintenance of chondrocytes and participated in the process of OA. Conclusions Our data first showed the landscape of eccDNA in articular cartilage and preliminarily indicated the potential roles of eccDNA in OA.
Systematic Review and Meta-analysis of Studies Comparing Complete Capsular Closure Against Unrepaired Hip Capsules During Hip Arthroscopy
Authors: Dasari Suhas P. , Kasson Luke B. , Condon Joshua J. , Mameri Enzo S. , Kerzner Benjamin , Khan Zeeshan A. , Jackson Garrett R. , Gursoy Safa , Sivasundaram Lakshmanan , Hevesi Mario , Chahla Jorge 
Published: October 2023

Background: While the biomechanical importance of the hip capsule is well described, there remains controversy over the necessity of routine capsular closure after hip arthroscopy. Purpose: To perform a meta-analysis of clinical studies to compare pooled outcomes of complete hip capsular closure cohorts against unrepaired hip capsule cohorts. Study Design: Systematic review; Level of evidence, 3. Methods: The Cochrane Database of Systematic Reviews, Cochrane Register of Controlled Trials, PubMed, MEDLINE, Web of Science, CINAHL/EBSCO, and Scopus were queried in February 2022 for studies that directly compared clinical outcomes for hip arthroscopy patients treated with either complete capsular closure or an unrepaired capsule. Outcomes assessed were incidence of revision hip arthroscopy, incidence of subsequent conversion to total hip arthroplasty (THA), and improvement from baseline in modified Harris Hip Score (mHHS), Hip Outcome Score (HOS) activities of daily living (ADL), HOS sports specific (SS) subscale, Copenhagen Hip and Groin Outcome Score (HAGOS) ADL, and HAGOS SS subscale. A pooled weighted mean difference (WMD) was used to compare changes in mHHS. A pooled standardized mean difference (SMD) was used to compare changes in the ADL and SS outcomes. A pooled risk ratio (RR) was used to compare the probability of revision hip arthroscopy and conversion to THA based on capsular management. For pooled outcomes where heterogeneity was regarded as potentially unimportant, a fixed-effects model was implemented. For pooled outcomes with considerable heterogeneity, a random-effects model was implemented. Results: Of the 1896 records identified in our search, 11 studies (1897 patients) were included. A significantly higher improvement in mHHS (WMD, −3.72; 95% CI, −4.95 to −2.50; P < .00001) and ADL outcomes (SMD, −0.30; 95% CI, −0.54 to −0.07; P = .01) were seen after complete capsular closure. There was a significantly lower probability of subsequent revision hip arthroscopy (RR, 1.67; 95% CI, 1.14 to 2.45; P = .008) and conversion to THA (RR, 2.01; 95% CI, 1.06 to 3.79; P = .03) after complete capsular repair. There was no difference in SS outcomes (SMD, −0.02; 95% CI, −0.16 to 0.13; P = .81) between the 2 groups. Conclusion: This meta-analysis demonstrated that routine complete capsular closure after hip arthroscopy led to superior clinical outcomes relative to unrepaired hip capsules.
Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed
Authors: Mussa Lubaba Amede , Yadetie Diriba Muleta , Temesgen Endeshaw Abatenh , Tefera Anteneh Tesfaye , Gemeda Mesfin Tafesse 
Published: October 2023

Abstract
Background
Phytase catalyses the breakdown of complex organic forms of phosphorous into simpler forms by sequential hydrolysis of phosphate ester bonds to liberate the inorganic phosphate. Supplementation of feeds with bacterial phytase therefore could enhance the bioavailability of phosphorus and micronutrients. Hence, the aim of this study was to isolate and characterize phytase producing bacteria from rhizosphere soil, fresh poultry excreta, and cattle shed to evaluate their potential in improving poultry feeds. Phytase producing bacteria were isolated using wheat bran extract medium.

Results
A total of 169 bacterial isolates were purified and screened for phytase activity. Out of these, 36 were confirmed as positive for phytase enzyme activity. The bacterial isolates were identified by cultural, morphological, and biochemical features. The isolates were also identified by using 16 S rRNA gene sequencing. The bacterial isolates (RS1, RS8, RS10 and RS15) were provided with gene bank database accession numbers of MZ407562, MZ407563, MZ407564 and MZ407565 respectively. All isolates increased phytase production when cultured in wheat bran extract medium (pH 6) supplemented with 1% (wt/v) galactose and 1% (wt/v) ammonium sulphate incubated at 50oC for 72 h. Proximate composition analysis after supplementation of phytase showed that phytase supplementation improved bioavailability of phosphorus, calcium, potassium and sodium in poultry feed.

Conclusions
Overall, this study showed that the nutritional value of poultry feed can be improved using microbial phytase enzyme which reduces the cost of supplementation with inorganic phosphate.