bluuebunny/crossref_metadata_2025_split huggingface dataset:
A resource of single‐cell gene expression profiles in a planarian Dugesia japonica
Authors: Kashima Makoto , Komura Rei , Sato Yuki , Hashimoto Chikara , Hirata Hiromi 
Published: October 2023

AbstractThe freshwater planarian Dugesia japonica maintains an abundant heterogeneous cell population called neoblasts, which include adult pluripotent stem cells. Thus, it is an excellent model organism for stem cell and regeneration research. Recently, many single‐cell RNA sequencing (scRNA‐seq) databases of several model organisms, including other planarian species, have become publicly available; these are powerful and useful resources to search for gene expression in various tissues and cells. However, the only scRNA‐seq dataset for D. japonica has been limited by the number of genes detected. Herein, we collected D. japonica cells, and conducted an scRNA‐seq analysis. A novel, automatic, iterative cell clustering strategy produced a dataset of 3,404 cells, which could be classified into 63 cell types based on gene expression profiles. We introduced two examples for utilizing the scRNA‐seq dataset in this study using D. japonica. First, the dataset provided results consistent with previous studies as well as novel functionally relevant insights, that is, the expression of DjMTA and DjP2X‐A genes in neoblasts that give rise to differentiated cells. Second, we conducted an integrative analysis of the scRNA‐seq dataset and time‐course bulk RNA‐seq of irradiated animals, demonstrating that the dataset can help interpret differentially expressed genes captured via bulk RNA‐seq. Using the R package “Seurat” and GSE223927, researchers can easily access and utilize this dataset.
Design for Sharing
Authors: Madsen Thit Juul 
Published: October 2023

The world around us shapes the way we think and create ideas, and the way we create ideas forms the world around us. If that's true, then we'd better learn to share.The trend in innovation is co—collaboration, co‐living, co‐working, and co‐creating, where solutions are developed and created in new shared spaces of knowledge, insights, and ideas.
Critical Idealism as Method: Ernst Cassirer and the Philosophy of Symbolic Forms
Authors: Pedersen Esther Oluffa 
Published: October 2023

AbstractTo commemorate the centenary of Ernst Cassirer's Philosophy of Symbolic Forms this essay focuses on how Cassirer in the development of a distinctive philosophical method analyzed the newest development within philosophy and science. Discussing Einstein's theory of relativity and Russell's formal logic Cassirer found tools to expand the critique of reason into a critique of culture. The course of argumentation is as follows. At the outset Cassirer's outline of the idea of The Philosophy of Symbolic Forms in the 1920 book Einstein's Theory of Relativity is presented as a reaction to the increasing distance between theoretical physics and ordinary experience. The Philosophy of Symbolic Forms can be read as an answer to an inner methodological demand within critical idealism. I encircle this motif in Cassirer's comment that Plato's idealism, understood as the dianoia of thought, is reiterated in Kant's transcendental philosophy and Herman Cohen's reception of Kant. This leads to a discussion of how Cassirer breaks away from Cohen by the positive reception of Russell's symbolic logic. Finally, I present the theory of functional concepts developed by Cassirer (1910) in the book Substance and Function as a prerequisite for the conception of a plural but systematic philosophy of symbolic forms.
Mucocutaneous manifestations of chikungunya fever: an update
Authors: Vinay Keshavamurthy , Thind Anish , Mehta Hitaishi , Bishnoi Anuradha 
Published: October 2023

AbstractChikungunya is a viral disease transmitted by female Aedes mosquitoes that has been increasingly reported in many parts of the world across the geographical borders. In addition to fever and joint pain, mucocutaneous manifestations of chikungunya have been reported in 40–75% of infected patients. Dermatological manifestations of chikungunya are often under‐recognized and misdiagnosed as clinicians are not sensitized or educated regarding these. The early‐onset cutaneous manifestations of chikungunya fever, occurring within 1 month of the fever, include maculopapular rashes, vesiculobullous eruptions, Steven‐Johnson syndrome/toxic epidermal necrolysis‐like eruptions, flagellate lesions, scrotal dermatitis, oro‐genital ulcers, and exacerbation of preexisting dermatoses like psoriasis. Hyperpigmentation, lichenoid eruptions, diffuse hair fall, and exacerbation of acne usually occur as a late manifestation. Diagnosis of these mucocutaneous manifestations can be challenging as they often resemble other common dermatoses. This review article elaborates on various mucocutaneous manifestations of chikungunya fever, based on literature review and authors’ clinical experience.
Enrichment of pasta with Pangasianodon hypophthalmus protein isolate for improved sustainability, nutrition, and quality
Authors: Saini Mandeep , Singh Arashdeep , Sharma Tamanna , Surasani Vijay Kumar Reddy , Kumar Vikas , Bobade Hanuman 
Published: October 2023

SummaryThe study was conducted to formulate pasta with fish protein isolate (FPI) (0%, 2.5%, 5.0%, 7.5%, and 10.0%) and assessed its functional, cooking, textural, proximate, FTIR, and sensory attributes in comparison to control. Results showed that addition of FPI significantly influenced the functional properties. The protein (12.25%–20.79%) and ash (0.40%–3.21%) content increased significantly (P < 0.05), while cooking time, swelling index, and cooked weight decreased. The supplementation also increases the firmness (0.181–0.440 kg) and toughness (0.680–1.33 kg/s) of pasta. FTIR spectra show the presence of the amide I, II, and III groups, with peaks at 1636, 1542, and 1228 cm−1 with increased intensity as concentration increases. Pasta (uncooked and cooked) exhibits decrease in whiteness and increase in yellowness and redness with increased FPI levels. Pasta with 5% FPI was found acceptable by the panellists with an acceptable fish odour and highest overall acceptability scores.
Meta‐analysis: Microbial inactivation in milk using high‐pressure processing (HPP)
Authors: Kateh Su‐aibah , Purnomo Eko Hari , Hasanah Uswatun 
Published: October 2023

SummaryHigh‐pressure processing (HPP) is a method of food preservation where food is processed under extremely high pressure, leading to the inhibition of bacteria and enzymes and ensuring that food is safe for consumption. This meta‐analysis aims to assess the inactivation effects of HPP on the microbial counts in milk. Analyses were performed using a comprehensive dataset containing 26 studies selected using specific criteria. Data were analysed using a random‐effects model and results are presented as raw mean differences (RMD) with 95% confidence intervals (95% CI). Results showed that spore‐forming bacteria had the highest resistance to HPP, followed by Gram‐positive and then Gram‐negative bacteria. A temperature range of 25–55 °C for vegetative cells and 55–90 °C for spores, a pressure of 400–600 MPa, and increasing the treatment time further enhance the efficiency of microbial inactivation. The results showed that HPP has a significant impact on inactivation of microbial counts in milk.
Sensory, cognitive perception, and consumer liking of Mexican Persian lime (Citrus latifolia Tanaka): an online survey with pictures comparing consumer types and sale contexts
Authors: Castillo‐Martínez Susana Isabel , Díaz‐José Julio , Herrera‐Corredor José Andrés , Cabal‐Prieto Adán , Leyva‐Ovalle Otto Raúl , Murguía‐González Joaquín , Dimas‐García Carlos Humberto , Prinyawiwatkul Witoon , Ramírez‐Rivera Emmanuel de Jesús 
Published: October 2023

SummaryThe objective of the study was to evaluate the sensory and cognitive perception of different types of consumers and contexts of sale of Mexican Persian lime using digital images and an online survey. A questionnaire in Google® Forms was responded by 293 Mexican consumers grouped into housewives, conventional consumers, and lime producers who evaluated images of Mexican Persian lime in different sale contexts: farm; producer's house; local market; supermarket and sale at packing facilities Mexican Persian lime. Sensory attributes elicited by digital images were evaluated, and emotions and memories were collected using the check‐all‐that‐apply technique. The results showed that the producers used less sensory attributes for the characterisation of the Persian lime images. Persian lime images evoked more positive emotions (free, loving, calm, adventurous, nostalgic, happy, joyful, interested, active, satisfied, and warm), positive memories (traditional food, family, nature, climate, and birthplace), and few negative emotions and memories (worried, pain, and poverty). The liking of the three groups of consumers was oriented to the contexts of sale of Mexican Persian lime in the farm and supermarket.
Effects of Gnaphalium affine extract (GAE) and sodium pyrophosphate (PP) on •OH‐induced oxidation and the structure of myofibrillar proteins
Authors: Chang Haijun , Shi Yuanwei , Xiong Jie , Bo Zhaoying , Dai Yan 
Published: October 2023

SummaryThis study investigated the effects of the combination of Gaphalium affine extract (GAE) and sodium pyrophosphate (PP) on the structure and oxidative stability of porcine myofibrillar proteins (MPs) under oxidative stress. The results showed that GAE was rich in total flavonoids and total phenols (37.09% and 67.50%, respectively). GAE had strong free radical scavenging ability, especially for •OH, and the semi‐inhibitory concentration (IC50) of •OH reached as high as 3.59 μg/mL, which was slightly lower than that of the standard antioxidant vitamin C (Vc). The addition of GAE and PP significantly inhibited the formation of carbonyl groups compared to the control group (P < 0.05). The presence of GAE further promoted the loss of total sulphydryl groups by 3.52% compared to the oxidative control group (P < 0.05). However, PP and PP+G significantly inhibited the loss of total sulphydryl groups (P < 0.05). The further reduction in the carbonyl content, increase in the hydrogen bond content, inhibition of the conversion of sulphydryl groups to disulphide bonds, and decrease in the cross‐linking aggregation of corresponding proteins all indicate that GAE and PP could cooperatively regulate the oxidation of MPs. An appropriate amount of PP effectively modified the damage caused by GAE on both the secondary and tertiary structure of MPs to maintain their structural stability, however, a relatively higher concentration of PP led to a substantial degree of MP collapse and affected the quality of the proteins. These results suggest that the oxidative stability of MPs can be improved by the addition of an appropriate amount of GAE (0.2 mg/g protein) combined with PP (1 mM).
Silencing of heat shock factor 1 (HSF1) inhibits proliferation, invasion, and epithelial‐mesenchymal transition in oral squamous cell carcinoma
Authors: da Silva Luiz Arthur Barbosa , da Costa Lucas Melo , Massetti Ana Camila Pereira , de Lucena Pereira Laudenice , da Silveira Ericka Janine Dantas , Salo Tuula Anneli , Coletta Ricardo Della , da Costa Miguel Márcia Cristina 
Published: October 2023

AbstractBackgroundOral squamous cell carcinoma is characterized by high rates of morbidity and mortality. Evidence obtained for different types of cancer shows that tumor initiation, progression, and therapeutic resistance are regulated by heat shock factor 1. This research aimed to analyze the effects of heat shock factor 1 on the biological behavior of oral squamous cell carcinoma.MethodsClinicopathological and immunoexpression study of heat shock factor 1 in 70 cases of oral tongue SCC and functional assays by gene silencing of this factor in an oral tongue SCC cell line.ResultsHeat shock factor 1 was overexpressed in oral tongue SCC specimens compared to normal oral mucosa (p < 0.0001) and in the SCC15 line compared to immortalized keratinocytes (p < 0.005). No significant associations were observed between overexpression of heat shock factor 1 and clinicopathological parameters or survival rates of the oral tongue SCC cases in the present sample. In vitro experiments showed that heat shock factor 1 silencing inhibited cell proliferation (p < 0.005) and cell cycle progression, with the accumulation of cells in the G0/G1 phase (p < 0.01). In addition, heat shock factor 1 silencing reduced cell invasion capacity (p < 0.05) and epithelial‐mesenchymal transition, characterized by a decrease in vimentin expression (p < 0.05) and an increase in E‐cadherin expression (p < 0.001).ConclusionHeat shock factor 1 may exert several functions that help maintain cell stability under the stressful conditions of the tumor microenvironment. Thus, strategies targeting the regulation of this protein may in the future be a useful therapeutic tool to control the progression of oral squamous cell carcinoma.
Conducting Interview Projects in the US Congress: Analyzing the Methods of Experts in the Field
Authors: Steiman Daniel , Suhay Elizabeth 
Published: October 2023

ABSTRACTThe field of political science is seeing renewed interest in studying the US Congress via one‐on‐one interviews. Yet, the qualitative research methods literature on this topic has lagged behind, with few recent treatments available. The result is uncertainty regarding how best to access and interview Congress. In this study, we implement a novel study design, interviewing over 20 authors—who collectively represent nearly all Congressional qualitative interview studies from the past several decades—about their research practices. Whereas the existing literature focuses on lessons learned from one or two authors’ research experiences, this approach allows us to synthesize a wide range of researchers’ practices and perspectives, identifying areas of consensus and dispute and ultimately providing comprehensive advice to qualitative researchers. As interviewing Congress becomes increasingly difficult amidst growing political polarization and distrust of academics, this methodological advice comes at an opportune time for researchers studying the US Congress and beyond.
Resource partitioning in a cetacean community from Uruguayan waters
Authors: Valdivia Meica , Valenzuela Luciano O. , Berriel Verónica , Rodríguez Diego , Laporta Paula , Drago Massimiliano , Cani Alessandra , Bergamino Leandro 
Published: October 2023

AbstractThe study of resource partitioning within a community is central to understanding the processes that enable interspecific competition and coexistence. This study aimed to understand the resource partitioning and habitat preferences among odontocetes in Uruguay. We analyzed stable carbon and nitrogen isotopes data from the bone tissue of common dolphins (Delphinus delphis), Fraser's dolphins (Lagenodelphis hosei), false killer whales (Pseudorca crassidens), Lahille's bottlenose dolphins (Tursiops truncatus gephyreus), Burmeister's porpoises (Phocoena spinipinnis), and franciscana dolphins (Pontoporia blainvillei). Based on the isotopic values, false killer whale individuals were divided into two different ecological groups (ecotype 1 and 2). Isotopic values also suggest that franciscana dolphins, Burmeister's porpoises, Lahille's bottlenose dolphins, and false killer whales ecotype 1 feed on the continental shelf and/or high trophic level preys. At the other end of the gradient, values suggest Fraser's dolphins and false killer whales ecotype 2 use resources from the oceanic environment and/or lower trophic preys. Isotopic niche overlap was found between the common dolphin and Lahille's bottlenose dolphin and between Lahille's bottlenose dolphin and false killer whale ecotype 1. This work provides novel basic information about the trophic habit and feeding habitat of the odontocetes inhabiting one of the most important estuaries of South America.
Body mass index in parents and their adult offspring: A systematic review and meta‐analysis
Authors: Zhang Jie , Clayton Gemma L. , Overvad Kim , Olsen Anja , Lawlor Deborah A. , Dahm Christina C. 
Published: October 2023

SummaryObesity may track across generations, due to genetics and shared family environmental factors, or possibly intrauterine programming. However, many studies only assess associations between maternal body mass index (BMI) and offspring BMI in childhood. To determine whether maternal and paternal associations with offspring BMI differ and whether associations persist into adulthood, a systematic review and meta‐analysis was done. PubMed, Embase, Web of Science, and Google Scholar (to October 2022) were searched. Observational studies reporting associations between maternal or paternal BMI and adult offspring BMI were included. Offspring BMIs were reported as continuous or categorical measures. Forty‐six studies were included in the systematic review. Meta‐analyses were conducted using random‐effects models. Parental BMI was positively associated with offspring BMI in adulthood. The pooled mother–offspring standardized mean difference (SMD) was 0.23 (95% confidence interval [CI]: 0.20, 0.26), and father–offspring SMD was similar: 0.22 (95% CI: 0.19, 0.25) in adjusted models. Offspring of mothers with overweight or obesity had the same risk of higher BMI as offspring of fathers with overweight or obesity. If these associations are causal, they support interventions targeting all family members, rather than focusing solely on mothers, to obtain a healthy weight development among offspring.
Resting‐state cerebral blood flow and functional connectivity abnormalities in depressed patients with childhood maltreatment: Potential biomarkers of vulnerability?
Authors: Liu Sangni , Fan Dandan , He Cancan , Liu Xinyi , Zhang Haisan , Zhang Hongxing , Zhang Zhijun , Xie Chunming 
Published: October 2023

AimChildhood maltreatment (CM) is an important risk factor for major depressive disorder (MDD). This study aimed to explore the specific effect of CM on cerebral blood flow (CBF) and brain functional connectivity (FC) in MDD patients.MethodsA total of 150 subjects were collected including 55 MDD patients with CM, 34 MDD patients without CM, 19 healthy controls (HC) with CM, and 42 HC without CM. All subjects completed MRI scans and neuropsychological tests. Two‐way analysis of covariance was used to detect the main and interactive effects of disease and CM on CBF and FC across subjects. Then, partial correlation analyses were conducted to explore the behavioral significance of altered CBF and FC in MDD patients. Finally, a support vector classifier model was applied to differentiate MDD patients.ResultsMDD patients represented increased CBF in bilateral temporal lobe and decreased CBF in right visual cortex. Importantly, significant depression‐by‐CM interactive effects on CBF were primarily located in the frontoparietal regions, including orbitofrontal cortex (OFC), lateral prefrontal cortex (PFC), and parietal cortex. Moreover, significant FC abnormalities were seen in OFC‐PFC and frontoparietal‐visual cortex. Notably, the abnormal CBF and FC were significantly associated with behavioral performance. Finally, a combination of altered CBF and FC behaved with a satisfactory classification ability to differentiate MDD patients.ConclusionsThese results highlight the importance of frontoparietal and visual cortices for MDD with CM experience, proposing a potential neuroimaging biomarker for MDD identification.
That sounds awful! Does sound unpleasantness modulate the mismatch negativity and its habituation?
Authors: Ringer Hanna , Rösch Sarah Alica , Roeber Urte , Deller Julia , Escera Carles , Grimm Sabine 
Published: October 2023

AbstractThere are sounds that most people perceive as highly unpleasant, for instance, the sound of rubbing pieces of polystyrene together. Previous research showed larger physiological and neural responses for such aversive compared to neutral sounds. Hitherto, it remains unclear whether habituation, i.e., diminished responses to repeated stimulus presentation, which is typically reported for neutral sounds, occurs to the same extent for aversive stimuli. We measured the mismatch negativity (MMN) in response to rare occurrences of aversive or neutral deviant sounds within an auditory oddball sequence in 24 healthy participants, while they performed a demanding visual distractor task. Deviants occurred as single events (i.e., between two standards) or as double deviants (i.e., repeating the identical deviant sound in two consecutive trials). All deviants elicited a clear MMN, and amplitudes were larger for aversive than for neutral deviants (irrespective of their position within a deviant pair). This supports the claim of preattentive emotion evaluation during early auditory processing. In contrast to our expectations, MMN amplitudes did not show habituation, but increased in response to deviant repetition—similarly for aversive and neutral deviants. A more fine‐grained analysis of individual MMN amplitudes in relation to individual arousal and valence ratings of each sound item revealed that stimulus‐specific MMN amplitudes were best predicted by the interaction of deviant position and perceived arousal, but not by valence. Deviants with perceived higher arousal elicited larger MMN amplitudes only at the first deviant position, indicating that the MMN reflects preattentive processing of the emotional content of sounds.
Pantoea agglomerans: A rare infectious outbreak affecting maintenance hemodialysis patients in a tertiary care hospital
Authors: Hassan Danyal , Saleem Nida , Haneef Muhammad , Khan Merina , Aziz Maliha , Usman Muhammad 
Published: October 2023

AbstractBackgroundPantoea agglomerans is an environmental pathogen known to cause infection in immunocompromised individuals, particularly after thorn injuries. However, previous data showed few cases of human disease caused by contaminated medical products such as parenteral nutrition, anesthetic agents, blood, and peritoneal dialysis solutions. Infection in hemodialysis patients is rare. In this study, we presented a detailed account of several hemodialysis patients infected with this contagious pathogen and compared them with noninfected dialysis patients.MethodsWe retrospectively reviewed the hospital records of 105 hemodialysis patients. Seventeen of 105 patients were diagnosed with P. agglomerans infection. We carefully analyzed their entire in‐hospital course.ResultsAmong infected patients, 52.9% were male with a median age of 49 (IQR: 32–66) years. Compared to the noninfected patients, age below 50 years, prior kidney transplantation, prior immunosuppression and antibiotics use, and dialysis via a tunneled vascular catheter were the significant epidemiological features. Despite negative microbiological investigations, we suspect the possible infectious spread via infected central venous catheter was the likely infectious source. Most importantly, all patients responded well to intravenous antibiotics. Only two patients required the removal of the tunneled catheter. Their mortality rate was 0%.ConclusionP. agglomerans infection, although considered rare, is becoming increasingly prevalent among dialysis patients. Its occurrence must be appraised as an infectious outbreak rather than mere contamination. Prompt treatment, source identification, and early implementation of preventive strategies should always be the goal to curtail this infection at an early stage.