bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of metabolomics and network pharmacology technology to explain the effect mechanisms of Danggui Buxue decoction in vascular dementia
Authors: Fan Qin , Liu Xinhong , Zhang Yanying , Kang Wanrong , Si Shanshan , Zhang Hongmei 
Published: January 2024

AbstractDanggui Buxue decoction (DBD) is a traditional Chinese medicine herbal decoction that has a good therapeutic effect on vascular dementia (VaD). However, its pharmacodynamic substances and underlying mechanisms are ambiguous. The work aimed to decipher the pharmacodynamic substances and molecular mechanisms of DBD against VaD rats based on gas chromatography–mass spectrometry metabonomics, network pharmacology, molecular docking, and experimental verification. The results indicated that DBD significantly improved the learning abilities and cognitive impairment in the VaD rat model. Integration analysis of the metabolomics and network pharmacology approach revealed that DBD might primarily affect arachidonic acid (AA) and inositol phosphate metabolic pathways by regulating the platelet activation signaling pathways. Six core targets (TNF [tumor necrosis factor], IL‐6 [interleukin 6], PTGS2 [prostaglandin‐endoperoxide synthase 2], MAPK1, MAPK3, and TP53) in the platelet activation signaling pathways also had a good affinity to seven main active components (saponins, organic acids, flavonoids, and phthalides) of DBD through the verification of molecular docking. Enzyme‐linked immunosorbent assay results (ELISA) showed that the levels of TNF, IL‐6, PTGS2, thromboxane B2, and caspase‐3 in the platelet activation signaling pathway can be regulated by DBD. Our results indicated that DBD treated VaD mainly by modulating the platelet activation signaling pathway, and AA and inositol phosphate metabolism.
Dual regulation effect and mechanism of human myeloid‐derived suppressor cells on anticolorectal cancer cells activity of Vγ9Vδ2 T cells
Authors: Zhang Mengyu , Wu Yuanyuan , Qin Yahan , Shen Jie , Cui Zhao , Lei Fan , Zhang Ke , Li Baiqing , Liang Shujuan , Peng Meiyu 
Published: January 2024

AbstractMyeloid‐derived suppressor cells (MDSC) are a group of immature inhibitory cells of bone marrow origin. Human γδ T cells (mainly Vγ9Vδ2 T cells) have emerged as dominant candidates for cancer immunotherapy because of their unique recognition pattern and broad killing activity against tumor cells. Intestinal mucosal intraepithelial lymphocytes are almost exclusively γδ T cells, so it plays an important role in inhibiting the development of colorectal cancer. In this study, we investigated the effects and molecular mechanism of human MDSC on anticolorectal cancer cells activity of Vγ9Vδ2 T cells. Our results suggested that MDSC can reduce the NKG2D expression of Vγ9Vδ2 T cells through direct cell–cell contact, which is associated with membrane‐type transforming growth factor‐β. In contrast, MDSC can increase Vγ9Vδ2 T cells activation and production of IFN‐γ, perforin, Granzyme B through direct cell–cell contact. This may be related to the upregulation of T‐bet in Vγ9Vδ2 T cells by MDSC. However, MDSC had a dominant negative regulatory effect on the anticolorectal cancer cells activity of Vγ9Vδ2 T cells. Our study provides a theoretical basis for the immune regulatory function of human MDSC on γδ T cells. This will be conducive to the clinical development of a new antitumor therapy strategy.
The COSMHYC Project Series: Innovative Hydrogen Compression Solutions
Authors: Chahrouri Rami , Colomar David , Jeanmougin Antoine , Martinez-Abenojar Alberto 
Published: January 2024

AbstractIn order to be stored efficiently, hydrogen gas is commonly compressed to high pressures making compression a crucial step in the value chain. Existing mechanical compressors have efficiency and reliability drawbacks, while novel technologies require further development and demonstration to reach market maturity. Comprehensive research & development efforts on metal hydride compression have recently resulted in significant advancements, allowing to overcome the main issues. A hybrid compression concept, proposed by the consortium of the COSMHYC project series, benefits from the advantages of both mechanical and metal hydride compression technologies, while limiting their drawbacks. It is currently being tested and demonstrated at full‐scale for implementation in real life applications.
Hydrogen Interactions with Defects in Materials
Authors: Pundt Astrid , Wagner Stefan 
Published: January 2024

AbstractThis manuscript summarizes basic properties of hydrogen in materials, mainly metals, and resulting consequences for the materials properties, including recent developments. It emphasises to introduce into the field of hydrogen in metals in a focused manner. It addresses hydrogen solution in materials, hydride formation, hydrogen diffusion and permeation and the interaction of hydrogen with defects. The influence of hydrogen on the defect energies and consequences for the metal behaviour are addressed. Further, the influence of constraint conditions on the system properties is shortly discussed.
Exploring the epigenetic susceptibility mechanisms of lung cancer through DNA methylation markers
Authors: Rolfo Christian , Del Re Marzia , Russo Alessandro 
Published: January 2024

During lung carcinogenesis, different epigenetic changes occur and, among these, DNA methylation aberrations play a critical role, representing an appealing biomarker for cancer screening. In this Cancer issue, Zhao et al. systematically assessed the associations of genetically predicted DNA methylation CpGs with non–small cell lung cancer risk.
Analysis and design of a passive regenerative inductor‐capacitor‐diode snubber for DC link over‐voltage mitigation in Y‐source inverter
Authors: Diver Rodrigo P. , Santos Rafael , Gonçalves Flávio A. S. 
Published: January 2024

AbstractRenewable energy‐based distributed generation presents new challenges for power inverters, requiring greater flexibility, reliability, cost‐effectiveness, and efficiency. Impedance source network‐based inverters (INIs) are an attractive option for addressing some of these issues since they provide a single‐stage power conversion structure with buck‐boost capability, allowing them to manage shoot‐through conduction on the inverter arms. However, some INI topologies based on magnetic coupling, such as those of the Y‐source impedance network family, have problems related to overvoltages at the power switches because of the presence of unwanted leakage inductances in the coupled‐inductor windings. The insertion of the regenerative and passive LCD snubber in the impedance network circuit is a solution to mitigate this problem, but there is currently a lack of a clear and concise methodology to design its components, which hinders a wider adoption of this solution in different types of INI. This paper presents a comprehensive and analytical design for determining LCD snubber components. This study was carried out with the quasi‐Y‐source inverter, but the same methodology can be extended to other types of INI based on the Y‐source network.
Population genetics and genetic variation of Pomacea canaliculata (Gastropoda: Ampullariidae) in China revealed by sequence analyses of three mitochondrial genes
Authors: Yi Xi‐Long , Liu Jing , Cao Mei‐Ling , Xiong Jun , Deng Yuan‐Ping , Wang Hui‐Mei , Ma Ping‐Ping , Liu Guo‐Hua , Yang Hua 
Published: January 2024

AbstractThe Golden apple snail, Pomacea canaliculata, is one of the world's 100 worst invasive alien species that is best known for its damage to wetland agriculture. It also acts as an intermediate host of some zoonotic parasites such as Angiostrongylus cantonensis, posing threats to human public health and safety. Despite is being an important agricultural pest, the genetic information and population expansion history of this snail remains poorly understood in China. In this study, we analyzed the genetic variation and population genetics of P. canaliculata populations in seven regions of China based on molecular markers of three mitochondrial (mt) genes. A total of 15 haplotypes were recognized based on single mt cox1, nad1, and nad4, and eight haplotypes were identified using the concatenated genes. High haplotype diversity, moderate nucleotide diversity, low gene flow, and high rates of gene differentiation among the seven P. canaliculata populations were detected. Shanghai and Yunnan populations showed higher genetic flow and very low genetic differentiation. The results of Tajima's D, Fu's Fs, and mismatch distribution showed that P. canaliculata did not experience population expansion in China. Genetic distance based on haplotypes suggested that nad1 gene was more conserved than cox1 gene within P. canaliculata. The phylogenetic analyses showed there may be two geographical lineages in the Chinese mainland. The present study may provide a new genetic marker to analyze P. canaliculata, and results support more evidence for studying the genetic distribution of P. canaliculata in China and contribute to a deeper understanding of its population genetics and evolutionary biology.
Ortho‐C−H Methylation and Ipso‐C−O Alkenylation of Aryl Triflates via Catellani Strategy
Authors: Yang Lei , Fu Jianbin , Tan Qihang , Li Chenglong , Zhang Shuo , Li Chunya , Liu Long , Huang Tianzeng , Chen Tieqiao 
Published: January 2024

AbstractThe ortho C−H methylation and ipso C−O alkenylation of aryl triflates was regio‐selectively achieved in one pot by palladium/norbornene (NBE) synergistic catalysis. This work provides an efficient method for preparing poly‐substituted aromatics with (deuterated) methyl and alkenyl groups from the readily available phenoxides.
A gem‐Dibromocyclopropane Ring‐Expansion/Mizoroki‐Heck Cyclisation Route to Tetrahydrodibenzofurans
Authors: Hu Nan , Chen Yu , Zhang Yu‐Qi , Lan Ping , Hu Ya‐Jian , Ward Jas S. , Gardiner Michael G. , Banwell Martin G. 
Published: January 2024

AbstractReadily accessible, oxygenated 6,6‐dibromobicyclo[3.1.0]hexanes react with isovanillin in the presence of silver salts and an inorganic base to afford ring‐expanded/nucleophilic trapping products of various regio‐ and stereo‐chemical forms. Most such products undergo efficient, intramolecular Mizoroki‐Heck reactions to give the corresponding tetrahydrodibenzofurans. In certain cases, simple variations in the first step of such reaction sequences enables the preparation distinct ring‐expansion products that also cyclise to give tetrahydrodibenzofurans embodying alternative substitution patterns.
Collapse hybrid simulation for testing steel building columns subject to boundary condition changes
Authors: Skalomenos Konstantinos , Kurata Masahiro 
Published: January 2024

AbstractThis paper develops a hybrid simulation to experimentally evaluate the ultimate behavior of steel columns subject to boundary condition changes and bending moment redistribution due to the progressive seismic collapse of building structures. The proposed experimental method combines sub‐structuring test techniques and refined finite element (FE) analysis methods in a hybrid scheme. The inelastic behavior of steel moment‐resisting‐frames (MRF) is computationally simulated while ground‐floor columns are physically tested online with the FE frame analysis. Boundary conditions at the interface node between the column test and the frame analysis are reproduced with the aid of three actuators that impose axial loads and bending deformations to the column test. Two square tubular steel columns are tested as part of a five‐storey five‐bay steel MRF designed to employ two different heights of the ground floor (i.e., 4.6 and 5.1 m, respectively). The hybrid simulation revealed that individual failures of the peripherical frame members significantly alter the distribution of the bending moment along the height of the column test, thus changing the boundary conditions at its top end. The imposed moment distribution was further influenced by local buckling initiated at the base of the column test and ground‐floor beams and could cause the top end of the upper‐storey columns of the frame to buckle. In the MRF with 4.6 m‐height ground‐floor, this resulted in a global collapse mechanism that involved the ground floor and the second storey. In the MRF with 5.1 m‐height ground‐floor, a weak‐storey collapse mechanism was formed within the ground floor. Compared to a corresponding isolated column tested as cantilever using conventional methods, the columns tested with the proposed hybrid simulation exhibited a more favorable inelastic behavior primarily due to the more realistic loads and changes of boundary conditions which are ignored in conventional test methods.
Valley‐bottom wetlands as temporary buffers for source‐to‐sink dispersal of sediment and associated phosphorus in dryland landscapes
Authors: Wiener Kenwinn D. , Grenfell Suzanne E. 
Published: January 2024

AbstractSediment trapping in wetlands is an essential ecosystem service, with implications for downstream ecosystems and water users. There is however limited empirical evidence of the contemporary rates and magnitude of sediment trapping in valley‐bottom wetlands. Time‐averaged suspended sediment samples from the inlets and outlets of forestry‐ and agriculturally‐impacted valley‐bottom wetlands with contrasting morphometric characteristics were compared in terms of suspended sediment and associated total phosphorous (total P) fluxes over annual scales, a dataset that was limited by Covid travel constraints. Although both wetlands were net depositional, contemporary suspended sediment mass balances for the agriculturally‐impacted wetland revealed a temporal change in the amount of sediment trapped over two water years (2019/2020 and 2020/2021), with trapping efficacies of 91% and 24%, respectively. The proportion of sediment trapped in the water year of 2020/2021 within the adjacent wetland, with a small commercially forested catchment, was up to 4 times higher than the agriculturally‐impacted wetland, which drained a larger catchment. Rates of total P retention showed that the agriculturally‐impacted wetland was a net sink for phosphorus in 2019/2020, but shifted to a source of phosphorus in 2020/2021 as the export of suspended sediment was enhanced. However, this contrasts with the forestry‐impacted wetland, which was a net sink of sediment and associated phosphorus during the one‐year study period of 2020/2021. Overall, despite data constraints, this study suggests that the efficacy of valley‐bottom wetlands in the delivery of sediment trapping and phosphorus removal ecosystem services varies temporally and spatially. This variability is potentially related to the interaction between annual rainfall regimes, catchment size and wetland geomorphic character. The temporary nature of sediment recycling processes could serve to balance wetland dynamics by regulating vertical growth of valley floors and longitudinal slope stability and should be considered in catchment management and wetland restoration planning strategies.
Child health and its effect on adult social capital accumulation
Authors: Lebenbaum Michael , de Oliveira Claire , Gagnon France , Laporte Audrey 
Published: January 2024

AbstractAlthough studies have demonstrated important effects of poor health in childhood on stocks of human and health capital, little research has tested economic theories to investigate the effect of child health on social capital in adulthood. Studies on the influence of child health on adult social capital are mixed and have not used sibling fixed effects models to account for unmeasured family and genetic characteristics, that are likely to be important. Using the Add‐Health sample, health in childhood was assessed as self‐rated health, the occurrence of a physical health condition or mental health condition, while social capital in adulthood was measured as volunteering, religious service attendance, team sports participation, number of friends, social isolation, and social support. We used sibling fixed effects models, which attenuated several associations to non‐significance. In sibling fixed effects models there was significant positive effects of greater self‐rated health on participation in team sports and social support, and negative effect of mental health in childhood on social isolation in adulthood. These results suggest that children with poor health require additional supports to build and maintain their stock of social capital and highlight further potential benefits to efforts that address poor child health.
How do hospitals respond to payment unbundling for diagnostic imaging of suspected cancer patients?
Authors: Hayes Helen , Stokes Jonathan , Sutton Matt , Meacock Rachel 
Published: January 2024

AbstractPayments for some diagnostic scans undertaken in outpatient settings were unbundled from Diagnosis Related Group based payments in England in April 2013 to address under‐provision. Unbundled scans attracted additional payments of between £45 and £748 directly following the reform. We examined the effect on utilization of these scans for patients with suspected cancer. We also explored whether any detected effects represented real increases in use of scans or better coding of activity. We applied difference‐in‐differences regression to patient‐level data from Hospital Episodes Statistics for 180 NHS hospital Trusts in England, between April 2010 and March 2018. We also explored heterogeneity in recorded use of scans before and after the unbundling at hospital Trust‐level. Use of scans increased by 0.137 scans per patient following unbundling, a 134% relative increase. This increased annual national provider payments by £79.2 million. Over 15% of scans recorded after the unbundling were at providers that previously recorded no scans, suggesting some of the observed increase in activity reflected previous under‐coding. Hospitals recorded substantial increases in diagnostic imaging for suspected cancer in response to payment unbundling. Results suggest that the reform also encouraged improvements in recording, so the real increase in testing is likely lower than detected.
Awareness of eclampsia among rural tribal women of reproductive age
Authors: Chhabra S. , Kumar N. , Tejane H. 
Published: January 2024

AbstractBackgroundGlobally, eclampsia is the leading cause of maternal and neonatal morbidity and mortality.ObjectiveThe present community‐based study was conducted among rural tribal women of reproductive age in remote villages of central India to determine their awareness of eclampsia and its likely impact.MethodsThis cross‐sectional analytic study included randomly selected 4500 tribal women, between 15 and 45 years of age, residing in 140 villages in the proximity of one village with a health facility (study center), and who were willing to undergo a personal interview. In‐depth face‐to‐face interviews (each lasting 15–30 min) of study subjects regarding awareness, knowledge, practices, and perceptions about eclampsia were conducted using a predesigned tool completed by research assistants (not the subjects).ResultsOf the 4500 women interviewed, the majority (62.4%) were 20–29 years old, minimally educated (40.6%), laborers (41.3%), and of a low socioeconomic class (40.8%). Of all the participants, only 35.9% were aware of eclampsia, associated events during pregnancy, labor, and the immediate post‐delivery period; 81.7% of those who were aware understood about symptoms and signs like headache, blurring of vision, dizziness, swelling over the body, ad high blood pressure. Of all the women who knew about eclampsia, 73.9% were aware that the occurrence of convulsions during antenatal and postnatal periods was an emergency and required urgent management, whereas 88.4% were not aware that severe convulsions affected maternal and neonatal health seriously, only 38.2% knew that eclampsia was a preventable condition.ConclusionThere was lack of awareness about eclampsia in many women and, of those who knew, some were not aware that it was dangerous. There is a need for awareness among women and their families of the disorder, its impact, and what action is needed in case it occurs.
A novel three‐plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector
Authors: Huang Yonghui , Dong Qisheng , Liu Guotao , Wang Tian , Gu Wenhao , Tian Zhen , Ma Qiang , Zhang Shoutao 
Published: January 2024

AbstractSemliki Forest virus (SFV) viral replicon particles (VRPs) have been frequently used in various animal models and clinical trials. Chimeric replicon particles offer different advantages because of their unique biological properties. We here constructed a novel three‐plasmid packaging system for chimeric SFV/SIN VRPs. The capsid and envelope of SIN structural proteins were generated using two‐helper plasmids separately, and the SFV replicon contained the SFV replicase gene, packaging signal of SIN, subgenomic promoter followed by the exogenous gene, and 3′ UTR of SIN. The chimeric VRPs carried luciferase or eGFP as reporter genes. The fluorescence and electron microscopy results revealed that chimeric VRPs were successfully packaged. The yield of the purified chimeric VRPs was approximately 2.5 times that of the SFV VRPs (1.38 × 107 TU/ml vs. 5.41 × 106 TU/ml) (p < 0.01). Furthermore, chimeric VRPs could be stored stably at 4°C for at least 60 days. Animal experiments revealed that mice immunized with chimeric VRPs (luciferase) had stronger luciferase expression than those immunized with equivalent amount of SFV VRPs (luciferase) (p < 0.01), and successfully expressed luciferase for approximately 12 days. Additionally, the chimeric VRPs expressed the RBD of SARS‐CoV‐2 efficiently and induced robust RBD‐specific antibody responses in mice. In conclusion, the chimeric VRPs constructed here met the requirements of a gene delivery tool for vaccine development and cancer therapy.
Discretizing low‐intensity whole‐body vibration into bouts with short rest intervals promotes bone defect repair in osteoporotic mice
Authors: Matsumoto Takeshi , Hashimoto Keishi , Okada Hyuga 
Published: January 2024

AbstractContinuous administration of low‐intensity whole‐body vibration (WBV) gradually diminishes bone mechanosensitivity over time, leading to a weakening of its osteogenic effect. We investigated whether discretizing WBV into bouts with short rest intervals was effective in enhancing osteoporotic bone repair. Ten‐week‐old female mice were ovariectomized and underwent drill‐hole defect surgery (Day 0) on the right tibial diaphysis at 11 weeks of age. The mice underwent one of three regimens starting from Day 1 for 5 days/week: continuous WBV at 45 Hz and 0.3 g for 7.5 min/day (cWBV); 3‐s bouts of WBV at 45 Hz, 0.3 g followed by 9‐s rest intervals, repeated for 30 min/day (repeated bouts of whole‐body vibration with short rest intervals [rWBV]); or a sham treatment. Both the cWBV and rWBV groups received a total of 20,250 vibration cycles per day. On either Day 7 or 14 posteuthanasia (n = 6/group/timepoint), the bone and angiogenic vasculature in the defect were computed tomography imaged using synchrotron light. By Day 14, the bone repair was most advanced in the rWBV group, showing a higher bone volume fraction and a more uniform mineral distribution compared with the sham group. The cWBV group exhibited an intermediate level of bone repair between the sham and rWBV groups. The rWBV group had a decrease in large‐sized angiogenic vessels, while the cWBV group showed an increase in such vessels. In conclusion, osteoporotic bone repair was enhanced by WBV bouts with short rest intervals, which may potentially be attributed to the improved mechanosensitivity of osteogenic cells and alterations in angiogenic vasculature.