bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix-rigidity cooperates with biochemical cues in M2 macrophage activation through increased nuclear deformation and chromatin accessibility
Authors: Shin Seung Jae , Bayarkhangai Buuvee , Tsogtbaataar Khaliunsarnai , Yuxuan Meng , Kim Sang-Hyun , Kim Yong-Jae , Kim Daesan , Kim Dong-Hwee , Lee Jung Hwan , Hyun Jeongeun , Kim Hae-Won 
Published: February 2024

AbstractMacrophages encounter a myriad of biochemical and mechanical stimuli across various tissues and pathological contexts. Notably, matrix rigidity has emerged as a pivotal regulator of macrophage activation through mechanotransduction. However, the precise mechanisms underlying the interplay between mechanical and biochemical cues within the nuclear milieu remain elusive. Here we elucidate how the increased matrix rigidity drives macrophages to amplify alternatively-activated (M2 phenotype) signalings cooperatively with biochemical cues (e.g., IL4/13) through altered nuclear mechanics. Notably, we found that reconstructed podosome-like F-actins and contractility induced nucleus deformation, opening nuclear pores, which facilitates nuclear translocation of the key transcription factor STAT6. Furthermore, the altered nuclear mechanics increased chromatin accessibility induced by H3K9 methylation, particularly of M2-associated gene promoters. These cooperative events of the mechano-chemical signaling at the cytoskeletal-to-nuclear domains facilitated M2 transcriptional activation and cellular functions. We further evidenced the rigidity-primed M2 macrophages were immunosuppressive and accumulated in stiffened tumor tissues. This study proposes a mechanism by which matrix mechanics crosstalks with biochemical signals to potentiate macrophage activation through nuclear mechanosensing and chromatin modifications, offering insights into macrophage mechanobiology and its therapeutic modulations.
Photo-Uncaging Triggers on Self-Blinking to Control Single-Molecule Fluorescence Kinetics for Super-Resolution Imaging
Authors: Zheng Ying , Ye Zhiwei , Zhang Xue , Xiao Yi 
Published: February 2024

AbstractSuper-resolution imaging in a single-molecule localization approach has transformed the bulk fluorescence requirements to a single-molecule level, raising a revolution in the fluorophore engineering. Yet, it is a challenge to structurally devise fluorophores manipulating the single-molecule blinking kinetics. In this pursuit, we have developed a new strategy by innovatively integrating the photoactivatable nitroso-caging strategy into self-blinking sulfonamide, to forming a nitroso-caged sulfonamide rhodamine (NOSR). Our fluorophore demonstrated controllable self-blinking events upon photo-triggered uncaging release. This exceptional blink kinetics improved integrity in super-resolution imaging microtubules compared to self-blinking analogues. With the aid of paramount single-molecule fluorescence kinetics, we successfully reconstructed the axial morphology of mitochondrial outer membranes. We foresee that our synthetic approach of photoactivation and self-blinking would set a new avenue for devising rhodamines for super-resolution imaging.Abstract Figure
Cathepsin B regulates ovarian reserve quality and quantity via mitophagy by modulating IGF1R turnover
Authors: Mohanty Aradhana , Kumari Anjali , Kumar. S Lava , Kumar Ajith , Birajdar Pravin , Beniwal Rohit , Athar Mohd , Kumar P Kiran , Prasada Rao H.B.D. 
Published: February 2024

AbstractThe quality and quantity of the ovarian reserve are meticulously regulated through various cell death pathways to guarantee the availability of high-quality oocytes for fertilization. While apoptosis is recognized for contributing to maintaining ovarian reserve, the involvement of other cell death pathways remains unclear. Employing chemical genetics and proteomics, this study reveals the crucial involvement of Cathepsin B in maintaining the ovarian reserve. Results indicate that apoptosis and autophagy play pivotal roles, and inhibiting these pathways significantly increases follicle numbers. Proteomics reveals a dynamic shift from apoptosis to autophagy during follicular development, with Cathepsin B emerging as a key player in this transition. Inhibiting Cathepsin B not only mimics the augmented oocyte reserve observed with autophagy inhibition but also upregulated IGF1R and AKT-mTOR pathways without compromising fertility. Further, IGF1R inhibition partially compromised the protective effects of Cathepsin B inhibition on oocyte reserves, suggesting their interdependence. This association is further supported by the finding that Cathepsin B can degrade IGF1R in vitro. Moreover, the increased IGF1R levels enhance the oocyte mitochondrial membrane potential via transcriptional regulation of mitochondrial biogenesis and mitophagy genes. Remarkably, this Cathepsin B-dependent ovarian reserve maintenance mechanism is conserved in higher-order vertebrates. Cumulatively, our study sheds valuable light on the intricate interplay of autophagy, Cathepsin B, and growth factors in ovarian reserve maintenance, offering potential implications for fertility research.
Not a single path to success: alternative HRM configurations for well-performing small and medium-sized hotels
Authors: Zografou I. , Galanaki E. , Pahos N. , Deligianni I. 
Published: February 2024

PurposePrevious literature has identified human resources as a key source of competitive advantage in organizations of all sizes. However, Small and Medium-sized Enterprises (SMEs) face difficulty in comprehensively implementing all recommended Human Resource Management (HRM) functions. In this study, we shed light on the field of HRM in SMEs by focusing on the context of Greek Small and Medium-sized Hotels (SMHs), which represent a dominant private sector employer across the country.Design/methodology/approachUsing a fuzzy-set qualitative comparative analysis (fsQCA) and 34 in-depth interviews with SMHs' owners/managers, we explore the HRM conditions leading to high levels of performance, while taking into consideration the influence of internal key determinants.FindingsWe uncover three alternative successful HRM strategies that maximize business performance, namely the Compensation-based performers, the HRM developers and the HRM investors. Each strategy fits discreet organizational characteristics related to company size, ownership type and organizational structure.Originality/valueTo the best of the authors' knowledge this is among the first empirical studies that examine different and equifinal performance-enhancing configurations of HRM practices in SMHs.
Enabling student employability through volunteering: insights from police volunteers studying professional policing degrees in Wales
Authors: Pepper Ian , Rogers Colin , Turner James , Louis Nick , Williams Bronwen 
Published: February 2024

PurposeThis paper aims to explore perceptions and provides insights, from students who volunteer in policing whilst also studying the college of policing licenced professional policing degree (PPD) in Wales. It examines issues that act as enablers and blockers to volunteering in this public service, which also provides opportunities to develop their employability towards the careers to which they aspire. The paper provides insights from students and makes recommendations of interest to multiple stakeholders locally, regionally, nationally and internationally regarding attracting and recruiting volunteers.Design/methodology/approachFocus groups were conducted with students at three separate universities in the South and West of Wales. These undergraduate students were volunteers in different police forces and agreed to take part in connection with their experiences. The results were analysed using NVivo to establish commonalities.FindingsThe paper provides empirical insights concerning the issues surrounding the way those undergoing the process for recruitment as volunteers within policing are either hindered (blocked) or assisted (enabled). It identifies specific problematic areas as well as areas which have been of assistance. Policy makers, educators and recruiters should be aware of such blockers and enablers when considering adopting volunteering as an opportunity to enhance student employability. It also has resonance for other forms of volunteering in alternate public services.Research limitations/implicationsThis research is limited to those volunteers who are undertaking the licenced PPD at three separate universities in Wales. Although the research adds to the broader evidence-base with regards to volunteering and its use to enhance employability.Practical implicationsThe paper includes practical implications for multiple stakeholders including university programme educators, administrators and decision makers in policing with regards to volunteering, employability, programme structures and process management.Originality/valueThe research is based upon the experiences and perceptions of those individuals who are actually engaged in volunteering.
Co-development of client involvement in health and social care services: examining modes of interaction
Authors: Kurki Anna-Leena , Weiste Elina , Toiviainen Hanna , Käpykangas Sari , Ylisassi Hilkka 
Published: February 2024

PurposeThe involvement of clients in service encounters and service development has become a central principle for contemporary health and social care organizations. However, in day-to-day work settings, the shift toward client involvement is still in progress. We examined how health and social care professionals, together with clients and managers, co-develop their conceptions of client involvement and search for practical ways in which to implement these in organizational service processes.Design/methodology/approachThe empirical case of this study was a developmental intervention, the client involvement workshop, conducted in a Finnish municipal social and welfare center. The cultural-historical activity theory (CHAT) framework was used to analyze the development of client involvement ideas and the modes of interaction during the intervention.FindingsAnalysis of the collective discussion revealed that the conceptions of client involvement developed through two interconnected object-orientations: Enabling client involvement in service encounters and promoting client involvement in the service system. The predominant mode of interaction in the collective discussion was that of “coordination.” The clients' perspective and contributions were central aspects in the turning points from coordination to cooperation; professionals crossed organizational boundaries, and together with clients, constructed a new client involvement-based object. This suggests that client participation plays an important role in the development of services.Originality/valueThe CHAT-based examination of the modes of interaction clarifies the potential of co-developing client-involvement-based services and highlights the importance of clients' participation in co-development.
Autoethnographic reflections on creating inclusive and collaborative virtual places for academic research
Authors: Trifan Cristina-Alexandra , de Waegh Roxane , Zhang Yunzi , Ooi Can-Seng 
Published: February 2024

PurposeThis paper explores the collaborative dynamics and dimensions within a virtual multi-cultural and interdisciplinary workplace. The study focusses on the use of online communication technologies to enhance social inclusion and networking within academia.Design/methodology/approachThis study uses an autoethnographic approach to draw on the personal experiences of a team of four scholars, including three early-career researchers and a senior scholar. Their reflections on their academic positionality and the institutional constraints reveal both the strengths and vulnerabilities of collaborating in a virtual workplace.FindingsThe findings offer insights into the complexities of navigating social dynamics, such as delegating responsibilities, organising meetings across various time zones and encouraging continuous collaboration, inclusivity and effective communication during an extensive timeline. As a result, their experiences revealed that a virtual workplace culture with similar and different attributes to a “normal” workplace emerged.Originality/valueThe paper demonstrates how to create an effective and inclusive virtual workplace by exemplifying best practices in academia and providing practical guidance for individuals and institutions based on honest, co-produced autoethnographic reflections of the authors’ lived experiences.
Shaping brand attitudes through sustainability practices: a TSR approach
Authors: Jagani Sandeep , Saboorideilami Vafa , Tarannum Saraf 
Published: February 2024

Purpose
This study aims to investigate the conditional relationships among sustainability implementation, brand awareness, brand attitude and brand loyalty through the lens of transformative service research (TSR). The research also aims to explain how brand loyalty moderates the mediated effect of brand awareness and attitude in the context of social and environmental sustainability initiatives.

Design/methodology/approach
Using both primary and secondary data sources from 31 prominent service companies and their 6,891 customers, this paper investigates the impact of sustainability practices and brand awareness on customer attitude. The paper also examines the moderated mediation effect of brand loyalty, explaining how it alters brand attitudes in the context of sustainability implementation. Finally, the study conducts a comparative analysis of how environmental and social shape brand attitudes in loyal customers.

Findings
Sustainability implementation has a negative impact on both brand awareness and customer attitude. However, this negative influence is mitigated for highly brand-loyal customers, resulting in a positive brand attitude. Further, the comparative analysis reveals that social implementation positively influences brand attitude in high-loyalty contexts.

Research limitations/implications
This research uses subjective judgments of researchers regarding companies’ sustainability practices, combining them with customer attitudes gathered through survey questionnaires. Additionally, the data set comprises data from 31 large service companies, potentially limiting the generalizability of findings to large service companies. Nevertheless, this paper extends TSR into the realm of sustainability and branding.

Practical implications
The positive outcomes of sustainability implementation practices are most pronounced when customer loyalty toward a brand is strong. Social implementation has a more potent effect on brand attitude, particularly among loyal customers. Companies can tailor their sustainability efforts more effectively.

Originality/value
With the lens of TSR, this research deepens our understanding of how sustainability affects consumer psychology but also offers a methodological advancement by using advanced statistical models and a variety of data sources. The distinctiveness of this research is also highlighted in the examination of how environmental and social sustainability initiatives influence brand attitude, especially among customers who exhibit strong brand loyalty.
The formalization of microenterprises in middle-income countries: informal institutions as a mechanism to address institutional incongruence
Authors: Bravo-Ortega Claudio , Bustamante Carla , Egana del Sol Pablo , Symmes Felipe , Sexton José 
Published: February 2024

Purpose
The purpose of this study is to investigate the role of sales, as a proxy for size, in moderating the impact of institutional incongruence between formal and informal institutions on the formalization of microenterprises in middle-income countries in Latin America.

Design/methodology/approach
The paper uses a probit regression model to examine business formalization as a binary outcome of formal and informal institutions. Data was collected through interviews and surveys across 52 municipalities in the Metropolitan Region of Santiago, Chile. The study used a stratified sampling approach and was conducted between November 2022 and January 2023.

Findings
The results offer three key insights into the formalization of microenterprises in middle-income countries. First, we show that formal institutions do not significantly influence formalization decisions among microentrepreneurs in middle-income countries, challenging the traditional belief that formal institutions alone significantly influence formalization in these contexts. Second, we show that informal institutions are significant predictors of informality, especially among smaller microenterprises. Third, we highlight that the smaller the business, the stronger the negative effect of informal institutions on formalization, and thus, the institutional incongruence between formal and informal institutions decreases for larger businesses.

Originality/value
This paper contributes to management literature by shedding light on the drivers of formalization in middle-income countries, a departure from most formalization studies wherein the focus is primarily on low-income economies. The findings suggest that policymakers in middle-income countries should focus on enabling microenterprise growth through sales, rather than targeting specific demographic groups or relying solely on formal institutional enforcement to promote formalization.
Hotspots in the EU‐27 and Economic Consequences of the 2022 Spring‐Summer Drought
Authors: Pinke Zsolt , Ács Tamás , Kalicz Péter , Kern Zoltán , Jambor Attila 
Published: February 2024

SummaryThe year 2022 will be remembered as a year of severe drought in Europe. Production losses on account of the drought from the six crops representing the most extensive harvested area may even have run to an estimated 13 billion euros in the EU‐27. Considering the ratio of the six crops in all croplands, the total revenue loss to cropland farming may have reached twice as much (25–30 billion euros). This figure indicates the magnitude of the challenge represented by climate change to the continent. While most of Europe suffered from severe drought in the spring and summer of 2022, the agricultural losses were region‐specific and were concentrated in the Western Mediterranean and Carpathian‐Balkan regions. Maize and sunflower suffered the highest losses, while data for wheat, barley, rapeseed, and rye showed an ambivalent picture. Losses in production of cropland farming associated with extremely high market prices have even reached 1–2 per cent of GDP in some countries in the Carpathian‐Balkan region. The paradox of last year was that despite drought hitting cropland farming severely, agriculture in the European Union has created a very high gross added value due to skyrocketing food prices.
Concurrently assessing water supply and demand is critical for evaluating vulnerabilities to climate change
Authors: Hall Sonia A. , Whittemore Aaron , Padowski Julie , Yourek Matthew , Yorgey Georgine G. , Rajagopalan Kirti , McLarty Sasha , Scarpare Fabio V. , Liu Mingliang , Asante‐Sasu Collins , Kondal Ashish , Brady Michael , Gustine Rebecca , Downes Melissa , Callahan Michael , Adam Jennifer C. 
Published: February 2024

AbstractAligning water supply with demand is a challenge, particularly in areas with large seasonal variation in precipitation and those dominated by winter precipitation. Climate change is expected to exacerbate this challenge, increasing the need for long‐term planning. Long‐term projections of water supply and demand that can aid planning are mostly published as agency reports, which are directly relevant to decision‐making but less likely to inform future research. We present 20‐year water supply and demand projections for the Columbia River, produced in partnership with the Washington State Dept. of Ecology. This effort includes integrated modeling of future surface water supply and agricultural demand by 2040 and analyses of future groundwater trends, residential demand, instream flow deficits, and curtailment. We found that shifting timing in water supply could leave many eastern Washington watersheds unable to meet late‐season out‐of‐stream demands. Increasing agricultural or residential demands in watersheds could exacerbate these late‐season vulnerabilities, and curtailments could become more common for rivers with federal or state instream flow rules. Groundwater trends are mostly declining, leaving watersheds more vulnerable to surface water supply or demand changes. Both our modeling framework and agency partnership can serve as an example for other long‐term efforts that aim to provide insights for water management in a changing climate elsewhere around the world.
The development and evaluation of dose‐prediction tools for allopurinol therapy (Easy‐Allo tools)
Authors: Wright Daniel F. B. , Hishe Hailemichael Z. , Stocker Sophie L. , Dalbeth Nicola , Horne Anne , Drake Jill , Haslett Janine , Phipps‐Green Amanda J. , Merriman Tony R. , Stamp Lisa K. 
Published: February 2024

AbstractAimsDose escalation at the initiation of allopurinol therapy can be protracted and resource intensive. Tools to predict the allopurinol doses required to achieve target serum urate concentrations would facilitate the implementation of more efficient dose‐escalation strategies. The aim of this research was to develop and externally evaluate allopurinol dosing tools, one for use when the pre‐urate‐lowering therapy serum urate is known (Easy‐Allo1) and one for when it is not known (Easy‐Allo2).MethodsA revised population pharmacokinetic‐pharmacodynamic model was developed using data from 653 people with gout. Maintenance doses to achieve the serum urate target of <0.36 mmol L−1 in >80% of individuals were simulated and evaluated against external data. The predicted and observed allopurinol doses were compared using the mean prediction error (MPE) and root mean square error (RMSE). The proportion of Easy‐Allo predicted doses within 100 mg of the observed was quantified.ResultsAllopurinol doses were predicted by total body weight, baseline urate, ethnicity and creatinine clearance. Easy‐Allo1 produced unbiased and suitably precise dose predictions (MPE 2 mg day−1 95% confidence interval [CI] −13‐17, RMSE 91%, 90% within 100 mg of the observed dose). Easy‐Allo2 was positively biased by about 70 mg day−1 and slightly less precise (MPE 70 mg day−1 95% CI 52‐88, RMSE 131%, 71% within 100 mg of the observed dose).ConclusionsThe Easy‐Allo tools provide a guide to the allopurinol maintenance dose requirement to achieve the serum urate target of <0.36 mmol L−1 and will aid in the development of novel dose‐escalation strategies for allopurinol therapy.