bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial structure of overlying rock strata and advanced abutment stress evolution in deep filling mining: A case study
Authors: Chen Yang , Sun Xikui , Gu Peng , Li Guoying , Fan Deyuan , Dong Chunyu , Zhang Yu , Wang Hao , Liu Chuancheng , Liu Xuesheng 
Published: October 2023

Abstract
Filling mining is one of feasible methods to prevent rock burst in coal mining, which is a meaningful topic in the field. In this paper, we first described the filling mining effect based on 'equivalent mining height' and analyzed the evolution of overlying rock strata. It was found that time-space hysteresis occurred during the movement of overlying rock strata in filled working face. (Movement of overlying rock strata took a period of time to reach the location of traditional caving) On this basis, mechanical relationship of dynamic transformation among 'filling rate-strata movement-abutment stress' was obtained by analyzing the coal stress characteristics. Then, advanced abutment stress estimation model of filled working face in deep coal mine was established. Finally, the C5301 working face of Yunhe coal mine was taken as the engineering background, example calculation and field monitoring were carried out. The results showed that the influence range of the advanced abutment stress is 91 ~ 97 m, and the peak value is 41.1 MPa, which is 21 ~ 50 m away from the working face. In addition, large pressure step and long interval time were the characteristics of time-space hysteresis in the filled working face. This study could provide guidance and reference for overlying rock strata and advanced abutment stress evolution in deep filling mining under the same or similar conditions.
A role for the ATP-dependent DNA ligase Lig E of Neisseria gonorrhoeae in biofilm formation
Authors: Pan Jolyn , Hicks Joanna , Williamson Adele 
Published: October 2023

Abstract
The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection characteristics. This demonstrates that N. gonorrhoeae Lig E is a non-essential gene and its deletion does not cause defects in replication or survival of DNA-damaging stressors. Knock-out strains were partially defective in biofilm formation on an artificial surface as well as adhesion to epithelial cells which coupled with the predicted extracellular/ periplasmic location of Lig E indicates a role in extracellular DNA joining. In addition to in vivo characterisation, we have recombinantly expressed and assayed N. gonorrhoeae Lig E and determined the crystal structure of the enzyme-adenylate engaged with DNA substrate in an open non-catalytic conformation, providing insight into the binding dynamics of these minimal DNA ligases.
Evaluating the Correlation between Serum Prostate-specific antigen Values and Gleason Score of the Prostate Carcinoma with Gallium 68- Prostate-specific membrane antigen Uptake in the Prostate Tumor in Primary Staging
Authors: Momeni Amir , Soltani Salman , Ghorbani Hamidreza , Tafazoli Nooshin , Kabiri Mona , Dadgar Habibollah , Golestani Zahra Bakhshi , Norouzbeigi Nasim , Kakhki Vahidreza Dabbagh , Sadeghi Ramin , aghaee atena 
Published: October 2023

Abstract
Objective
Gallium-68 - prostate-specific membrane antigen (Ga68-PSMA) PET/CT plays a crucial role in staging, evaluating treatment, and diagnosing local recurrence of prostate tumors. The National Comprehensive Cancer Network ( NCCN) guidelines have recently recommended it as the preferred staging method for unfavorable intermediate and high-risk patients. This study aims to evaluate the correlation between serum prostate-specific antigen (PSA) levels and the Gleason score of prostate tumors with the findings of Ga 68-PSMA PET/CT scans in primary staging.
Methods
In this cross-sectional study, newly diagnosed prostate cancer patients referred for Ga68-PSMA PET/CT whole-body scans were enrolled. The scans were performed at three-time points (early, whole-body, and late). The standardized uptake value of Ga68-PSMA in the primary tumor and its association with serum PSA levels, tumor Gleason score, and presence of tumor metastasis were evaluated and analyzed.
Results
Forty-nine male patients, 65 years old on average (interquartile range (IQR): 59–69) were included in the study. Of these patients, 43.9% were classified as high-risk based on their Gleason score (8–10). The median serum PSA level was 17 ng/mL (IQR: 8.02–42.10). PSA levels correlated with the maximum standardized uptake value (SUVmax) of the tumor in the whole-body stage of the scan (p < 0.05; r = + 0.392) but not in the early or late stages (p > 0.05). Gleason scores positively correlated with tumor SUVmax in all three scan stages (p < 0.05).
Conclusions
Our results indicate that the Gleason score significantly correlates with the SUVmax of the prostate tumor in all three-time points of the scan. However, serum PSA levels only correlate with the tumor SUVmax in the whole-body stage of the scan, not in the early and late stages. In the whole-body stage, a higher SUVmax is significantly associated with higher Gleason scores, bladder wall, invasion of the seminal vesicle, and lymph node metastasis (p < 0.05).
Different Patient Versus Provider Perspectives on Living with Cushing’s Disease
Authors: Halstrom Amanda , Lin I-Hsin , Lin Andrew , Cohen Marc , Tabar Viviane , Geer Eliza 
Published: October 2023

Abstract
Context: Patients with Cushing’s disease (CD) face challenges living with and receiving appropriate care for this rare, chronic condition. Even with successful treatment, many patients experience ongoing symptoms and impaired quality of life (QoL). Different perspectives and expectations between patients and healthcare providers (HCPs) may also impair well-being.
Objective: To examine differences in perspectives on living with CD between patients and HCPs, and to compare care goals and unmet needs.
Design: Memorial Sloan Kettering Pituitary Center established an annual pituitary symposium for pituitary patients and HCPs. Through anonymous pre-program surveys distributed at the 2020 and 2022 symposia, patients and HCPs answered questions related to their own sense, or perception of their patients’ sense, of hope, choice, and loneliness in the context of living with CD.
Participants: From 655 participants over two educational events, 46 patients with CD and 116 HCPs were included. Median age of both groups was 51 years. 78.3% of the patients were female vs. 53.0% of the HCPs.
Results:More patients than HCPs reported they had no choices in their treatment (21.7% vs. 0.9%, P<0.001). More patients reported feeling alone living with CD than HCPs’ perception of such (60.9% vs. 45.5%, P=0.08). The most common personal care goal concern for patients was ‘QoL/mental health,’ vs. ‘medical therapies/tumor control’ for HCPs. The most common CD unmet need reported by patients was ‘education/awareness’ vs. ‘medical therapies/tumor control’ for HCPs.
Conclusions: CD patients experience long term symptoms and impaired QoL which may in part be due to a perception of lack of effective treatment options and little hope for improvement. Communicating experiences and care goals may improve long term outcomes for CD patients.
Seed protein biotyping in Amaranthus species: A tool for rapid identification of weedy amaranths of concern
Authors: Murphy Maxime , Hubert Julia , Wang Ruojing , Galindo-González Leonardo 
Published: October 2023

Abstract
Background:
The Amaranthus genus contains at least 20 weedy and invasive species, including Amaranthus palmeri (palmer’s amaranth) and Amaranthus tuberculatus (tall waterhemp), two species of regulatory concern in North America, impacting production and yield in crops like corn, soybean and cotton. Amaranthus tuberculatus is regulated in Canada with limited establishment, while current climate models predict a range expansion of A. palmeri impacting crop growing areas in Ontario, Quebec and Manitoba. Since many Amaranthus species are similar in their morphology, especially at the seed stage, this demands the development of additional methods that can efficiently aid in the detection and identification of these species.
Protein biotyping using Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) has been traditionally used to identify microorganism species, races and pathotypes. Major protein fractions extracted from an organism, ionized and run through a biotyper using mass spectrometry, result in protein spectra that represent a fingerprint at the species or lower taxonomic rank, providing an efficient molecular diagnostics method.
Here we use a modified protein biotyping protocol to extract major protein fractions from seeds of the family Brassicaceae to test our protocol, and then implemented the standardized approach in seeds from Amaranthusspecies. We then created a database of Amaranthus protein spectra that can be used to test blind samples for a quick identification of species of concern.
Results:
We generated a protein spectra database with 16 Amaranthus species and several accessions per species, spanning target species of regulatory concern and species which are phylogenetically related or easily confused at the seed stage due to phenotypic plasticity. Testing of two Amaranthus blind sample seed sets against this database showed accuracies of 100% and 87%, respectively.
Conclusions:
Our method is highly efficient in identifying Amaranthus species of regulatory concern. The mismatches between our protein biotyping approach and phenotypic identification of seeds are due to absence of the species in the database or close phylogenetic relationship between the species.
While A. palmeri cannot be distinguished from A. watsonii, there is evidence these two species have the same native range and are closely related.
The relationship between self-stigma and quality of life in long-term hospitalized patients with schizophrenia: A cross-sectional study
Authors: 刘 福权 , Deng Hu , Hu Na , Huang Wenqian , Wang Hong , Zhou Xuanzi , Liu Lin , Chai Jiabao , Li Ying 
Published: October 2023

Abstract
Objective: To investigate self-stigma's influence on schizophrenia patients' quality of life and its mediated impact by various factors.
Methods: This study adopted a cross-sectional design and randomly selected 170 hospitalized patients with schizophrenia for evaluation. The assessment tools included the Positive and Negative Syndrome Scale (PANSS), Self-Esteem Inventory (SEI), Connor-Davidson Resilience Scale (CD-RISC), Internalized Stigma of Mental Illness Scale (ISMI), Schizophrenia Quality of Life Scale (SQLS), Coping Questionnaire for Schizophrenia Patients (CQSP), and Social Support Rating Scale (SSRS). Correlation analysis, regression analysis, and mediation analysis were used to test the correlation and mediation effects.
Results: Self-shame hada significant impact on quality of life (T = 8.13, p = 0.00). Self-esteem (T = -2.11, p = 0.04) and coping strategies, specifically problem-solving (T = -2.58, p = 0.01) and avoidance (T = 4.65, p= 0.00), have a greater impact on self-shame. When self-shame is used as a mediator, the problem-solving factor in coping strategies has an indirect effect on quality of life, which is significant (AB = -0.16, P = 0.02), while the avoidance factor in coping strategies has a direct effect on quality of life, which is significant (C' = 0.54, p < 0.001), and an indirect effect, which is also significant (AB = 0.25, p < 0.001).
Conclusion: Self-stigma has a significant impact on quality of life. Clinical work to improve the quality of life of patients with schizophrenia should focus on self-stigma and factors that affect self-stigma, especially self-esteem and coping strategies.
Influence of MnO on gamma attenuation characteristics of modified borate bioglass
Authors: Awad W. M. , Ziada O. E. , Meikail M. S. , Abdelghany A.M. 
Published: October 2023

Abstract
Modified borate Hench glass was successfully synthesized with the composition of (xMnO-(42.5-x)B2O3-28.7CaO-26Na2O-2.8P2O5) where x = (0, 0.1, 0.2, 0.3, 0.4, 0.6, and 0.8) mol% via melting technique and investigated by using different techniques. The density, molar volume, and several physical parameters were calculated. The density has nonlinear behavior by increased MnO due to the manganese ions' nonlinear behavior since they have numerous stable valence states, while the molar volume was increased. The UV/Vis measurement produced a peak that indicated the presence of manganese in Mn3+. The radiation shielding properties of the prepared samples were calculated theoretically using the Phy-X program at energies from 0.015 to 1.5 MeV. Linear and mass attenuation coefficients, as well as half value layer (HVL), tenth value layer (TVL), mean free path (MFP), effective conductivity (Ceff), and exposure buildup factor (EBF), were assessed. The results show that the shielding parameters are affected by MnO concentration and photon energy. Based on the results, it was determined that the synthesized glass sample containing 0.2 mol % MnO has the best optical properties and shielding capabilities, making it great for optical and shielding applications.
Spectral and conformational characteristics of phycocyanine associated with changes of medium pH
Authors: Parshina E.Yu. , Lee V. , Yusipovich A.I. , Gvozdev D. A. , Yang He , Pirutin S.K. , Klimanova E. A. , Maksimov E.G. , Maksimov G.V. 
Published: October 2023

Abstract
C-phycocyanin (C-PC) is the main component of water-soluble light-harvesting complexes of cyanobacteria. These complexes are involved in the absorption of quantum energy and the transfer of electron excitation energy to photosystems. A specific environment of C-PC chromophoric groups is provided by the protein matrix structure including protein-protein contacts between different subunits. Registration of C-PC spectral characteristics and the kinetics of a fluorescence anisotropy damping have revealed a significant pH influence on the chromophore microenvironment: at pH 5.0, a chromophore is more significantly interacts with a solvent, whereas at pH 9.0 the chromophore microenvironment becomes more viscous. Conformations of a chromophore and a C-PC protein matrix have been studied by Raman and infrared spectroscopy. A decrease in the medium pH results in changes in the secondary structure of both C-PC and chromophore making the last one to be more convoluted.
Genetic Diversity and Population Structure Analysis of Vernonia [Vernonia galamensis (Cass.) Less L.] Germplasm in Ethiopia using ISSR Markers
Authors: Eshetu Gete , Mekonnen Tilahun , Mideksa Alemineh , Tesfaye Kassahun 
Published: October 2023

Abstract
Vernonia (Vernonia galamensis (Cass.) Less.) is a new potential industrial oil crop with high content of vernolic acid in the seed oil. Ethiopia is one of the few Vernonia rich African countries. Knowledge of the genetic structure of the crop is very important for its improvement and sustainable management. The present study was targeted to investigate genetic diversity and population structure of 60 Vernonia genotypes in Ethiopia representing 8 populations using ISSR markers. A wide range of genetic diversity indices were computed to determine the extents and patterns of genetic variation within the eight populations. All the used markers were highly informative with polymorphic information content ranging from 0.5 to 0.71 with overall mean of 0.51, indicating that they are useful genetic tools for genetic structure analyses. The within-populations Nei’s gene diversity ranged from 0.28 to 0.43 with an overall mean of 0.36, indicating existence of promising genetic variation to exploit through breeding to improve desired traits. Clustering, and Principal coordinate analysis did not sharply grouped the genotypes following their geographical areas of sampling. Population structure analysis revealed the presence of two sub-populations with lower degree of genetic admixture. In conclusion, the used ISSR markers were very valuable and highly informative to determine the extent and patterns of genetic diversity and population structures of Vernonia. Among the studied populations, East Shoa and East Hararghe Vernonia populations showed relatively higher gene diversity indicating that these area could be targeted for identifying super performing Vernonia genotypes for breeding program and conservation purposes.
Pretherapeutic factors predicting conversion surgery in unresectable pancreatic ductal adenocarcinoma: a retrospective study
Authors: Higashi Toshiya , Murase Katsutoshi , Watanabe Daichi , Ishihara Takuma , Yokoi Ryoma , Kuno Masashi , Fukada Masahiro , Iwashita Takuji , Tajima Jesse Yu , Kiyama Shigeru , Tanaka Yoshihiro , Okumura Naoki , Shimizu Masahito , Matsuhashi Nobuhisa 
Published: October 2023

Abstract
Background
Recently, conversion surgery (CS) has been reported to improve the prognosis in patients with unresectable pancreatic ductal adenocarcinoma (UR-PDAC) with a favorable response to intense chemotherapy or chemoradiotherapy. However, few pretherapeutic parameters predict the attainability of CS in patients with UR-PDAC. This study aimed to explore the pretherapeutic predictors for the attainability of CS in patients with UR-PDAC.
Methods
We retrospectively evaluated 130 patients with UR-PDAC treated at our institute from January 2015 to December 2021. The CS was performed only in patients with UR-PDAC who could expect R0 resection in the multidisciplinary meeting. The primary outcome of the study was CS. Survival analysis was performed using the Simon and Makuch’s modified Kaplan–Meier method. The hazard ratio (HR) was estimated using a time-varying Cox regression model. The association between each predictor and CS was evaluated using the univariable and age-adjusted Fine-Gray sub-distribution hazard model. The bootstrap bias-corrected area under the receiver operating characteristic curve analysis for predicting the CS was used to assess the cut-off values for each predictor. The cumulative incidence rate was calculated with CS as the outcome when divided into two groups based on the cut-off value of each pretherapeutic predictor.
Results
Among the 130 patients included in the analysis, only 14 (10.8%) patients underwent CS. The median survival time was significantly longer in patients who underwent CS compared with patients without CS (56.3 versus 14.1 months; p < 0.001). The univariable and age-adjusted Fine-Gray sub-distribution hazard regression showed that the total protein (TP) (HR 2.81, 95% confidence interval [CI] 1.19–6.65; p = 0.018), neutrophil-to-lymphocyte ratio (NLR) (HR 0.53, 95% CI 0.31–0.90; p = 0.020), and lymphocyte-to-monocyte ratio (LMR) (HR 1.28, 95% CI 1.07–1.53; p = 0.006) were statistically significantly associated with CS. Moreover, TP ≥ 6.8, NLR < 2.84, and LMR ≥ 3.87 were associated with a higher cumulative incidence of CS.
Conclusions
Pretherapeutic TP, NLR, and LMR are clinically feasible biomarkers for predicting the attainability of CS in patients with UR-PDAC.
Green Energy Transition Using Torrefied and Treated Biomass in Indian Thermal Power Plants
Authors: Kulshreshtha Amit , Mallik Shuvadip , Saxena Kanchan , Dhakate Sanjay 
Published: October 2023

Abstract
The present work examines the prospects of using biomass-based fuels for the complete replacement of coal in Indian pulverized coal thermal power plants (PC-TPPs) without substantial plant modifications. Replacing coal with biomass-based fuel is the fastest way to green energy transition with several associated benefits like air quality improvement, waste elimination, and reduction of other harmful emissions. It also avoids the need for storage peculiar to high levels of renewable grid integration using solar and wind. However, substituting biomass-based fuels for coal requires them to have similar properties. We investigated torrefaction and washing of biomass wastes with parameter and treatment variations. Results indicate that the fuel properties of rice straw after torrefaction and washing resemble domestic Indian coal, and it can potentially replace the coal completely in large thermal power plants. Similarly, other biomasses studied, viz. Lantana camara can, grass and tree leaf litter can replace imported coal.
DrugGPT: A Knowledge-Grounded Collaborative Large Language Model for Evidence-based Drug Analysis
Authors: Liu Fenglin , Zhou Hongjian , Zhang Wenjun , Huang Guowei , Clifton Lei , Eyre David , Luo Haochen , Liu Fengyuan , Branson Kim , Schwab Patrick , Wu Xian , Zheng Yefeng , Thakur Anshul , Clifton David 
Published: October 2023

Abstract
The authors have requested that this preprint be removed from Research Square.
Precision local anomaly positioning technology for large complex electromechanical systems
Authors: Zhao Yaping 
Published: October 2023

In recent years, Prognostics Health Management (PHM) technology has become an important reference technology in fields such as avionics and electromechanical systems due to its ability to reduce costs and achieve state based maintenance and autonomous support. However, with the operation of large and complex electromechanical systems (ES), the data generated gradually ages the status of components, and traditional PHM technology is difficult to solve the problem of electromechanical system components becoming more complex. Based on this, this study takes the hydraulic actuator cylinder as an example to construct a local component fault detection model. Firstly, fault data features are extracted using wavelet packet energy spectrum, and then a fault detection model is constructed based on support vector machine (SVM). In response to the shortcomings of SVM, a smooth support vector machine (SSVM) is proposed to replace SVM, and an improved crow search algorithm (ICSA) is used to improve SVM. Finally, an intelligent detection model for hydraulic actuator cylinder faults based on ICSA-SSVM was constructed based on the above algorithms. The experimental results show that the ICSA-SSVM model has the fastest Rate of convergence, among which, the positioning accuracy is 0.96, the fitting degree is 0.984, the fault detection accuracy is 99.16 %, the recall value is 94.52 %, and the AUC value is 0.986, all of which are better than the existing fault detection models. From this, it can be seen that the precise local anomaly localization technology for large-scale complex electromechanical systems based on the ICSA-SSVM algorithm proposed in this study can improve the efficiency and accuracy of fault detection, achieve accurate and intelligent detection of ES local anomalies, and have certain positive significance for the development of China’s industry.