bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between dynamic fluctuations in triiodothyronine levels and prognosis among critically ill patients within comprehensive intensive care units
Authors: Xu Yu , Xu Kang , Guo Jianying , Fang Mingxing , Wang Zhiyong 
Published: November 2023

ObjectiveDecrease in free thyroid hormone T3 (FT3) can be used as an independent prognostic indicator for the risk of death in ICUs. However, FT3 as a predictive marker is hindered by its accuracy. The study introduces the concept of dynamic FT3 data as a means to bolster the value of FT3 as a prognostic tool. Therefore, the aim of this study is to investigate the prognostic value of dynamic FT3 evolution in a comprehensive ICU setting, analyze the consistency between dynamic FT3 changes and variations in disease severity, and explore the feasibility of FT3 as an objective indicator for real-time clinical treatment feedback.MethodsEmploying a single-center prospective observational study, FT3 measurements were taken on multiple days following enrollment, corresponding clinical data were collected. To investigated the pattern of dynamic changes of FT3,its prognostic significance in forecasting the risk of 28-day mortality, the alignment between dynamic FT3 changes and variations in the Sequential Organ Failure Assessment (SOFA) score.ResultsThe survival group exhibited higher last FT3 levels compared to the lowest point (p&lt;0.05), while the death group did not show statistically significant differences (p&gt;0.05). The study also identifies the optimal correlation between FT3 and SOFA score at day 5 (optimal correlation coefficient -0.546).The ROC curve for FT3 at day 5 yielded an optimal AUC of 0.88, outperforming the SOFA score. The study categorizes FT3 curve patterns,Kaplan-Meier survival analysis of these patterns highlighted that the descending-type curve was significantly associated with increased risk of death (P&lt;0.001). Additionally, the research explores the consistency between changes in FT3 and SOFA scores. While overall consistency rates were modest, subgroup analyses unveiled that greater disease severity led to higher consistency rates.ConclusionsThis study introduces the concept of dynamic FT3 changes to augment its prognostic utility in comprehensive ICU settings. The research identifies day 5 as the optimal time point for predictive efficacy, the descending FT3 curve as indicative of poor prognosis. While overall consistency with SOFA scores is modest, the correlation strengthens with greater disease severity.
Research on the thermal performance of the condenser in a loop heat pipe anti-icing system
Authors: Zhao Yuanyuan , Li Guang , Dang Chaobin , Wu Hongqiang 
Published: November 2023

Loop Heat Pipes (LHPs) are efficient heat transfer devices, providing a highly efficient and energy-saving thermal control integrated management method for Unmanned Aerial Vehicles (UAVs) with their flexible structural design. In this paper, a conceptual design of LHP anti-icing system was proposed, using LHP to transfer waste heat to wing’s leading edge. The outer surface temperature of wing skin is one of the main indicators to measure the anti-icing effect. As a result, this paper mainly focused on the heat transfer process of the condenser section by experimental and numerical simulation methods. A stainless steel-nickel LHP was fabricated and tested at different conditions. Volume of Fluid (VOF) method and Lee model were adopted to simulate the condensation process. Results showed that increasing the heating power (from 35 W to 60 W) slowed down the condensation and lengthened the two-phase zone. However, when the angle of attack changes within the range of 0°–10°, the liquid-vapor distribution in the tube remains almost unchanged. The average error of surface temperature between experiment and simulation results is 3.6% and 2.5% for the heating power of 180 W and 60 W respectively. Additionally, because the volume of water droplets collected is the largest at the leading edge of the wing, we recommend arranging the LHP condenser tube inlet near the leading edge of the wing and increasing the density of pipe arrangement to achieve better anti-icing effect.
Peptidylarginine deiminase 2 citrullinates MZB1 and promotes the secretion of IgM and IgA
Authors: Geary Benjamin , Sun Bo , Tilvawala Ronak R. , Barasa Leonard , Tsoyi Konstantin , Rosas Ivan O. , Thompson Paul R. , Ho I-Cheng 
Published: November 2023

IntroductionMZB1 is an endoplasmic reticulum residential protein preferentially expressed in plasma cells, marginal zone and B1 B cells. Recent studies on murine B cells show that it interacts with the tail piece of IgM and IgA heavy chain and promotes the secretion of these two classes of immunoglobulin. However, its role in primary human B cells has yet to be determined and how its function is regulated is still unknown. The conversion of peptidylarginine to peptidylcitrulline, also known as citrullination, by peptidylarginine deiminases (PADs) can critically influence the function of proteins in immune cells, such as neutrophils and T cells; however, the role of PADs in B cells remains to be elucidated.MethodAn unbiased analysis of human lung citrullinome was conducted to identify citrullinated proteins that are enriched in several chronic lung diseases, including rheumatoid arthritis-associated interstitial lung disease (RA-ILD), chronic obstructive pulmonary disease, and idiopathic pulmonary fibrosis, compared to healthy controls. Mass spectrometry, site-specific mutagenesis, and western blotting were used to confirm the citrullination of candidate proteins. Their citrullination was suppressed by pharmacological inhibition or genetic ablation of PAD2 and the impact of their citrullination on the function and differentiation of human B cells was examined with enzyme-linked immunosorbent assay, flow cytometry, and co-immunoprecipitation.ResultsCitrullinated MZB1 was preferentially enriched in RA-ILD but not in other chronic lung diseases. MZB1 was a substrate of PAD2 and was citrullinated during the differentiation of human plasmablasts. Ablation or pharmacological inhibition of PAD2 in primary human B cells attenuated the secretion of IgM and IgA but not IgG or the differentiation of IgM or IgA-expressing plasmablasts, recapitulating the effect of ablating MZB1. Furthermore, the physical interaction between endogenous MZB1 and IgM/IgA was attenuated by pharmacological inhibition of PAD2.DiscussionOur data confirm the function of MZB1 in primary human plasmablasts and suggest that PAD2 promotes IgM/IgA secretion by citrullinating MZB1, thereby contributing to the pathogenesis of rheumatoid arthritis and RA-ILD.
Comparative study of virulence potential, phylogenetic origin, CRISPR-Cas regions and drug resistance of Escherichia coli isolates from urine and other clinical materials
Authors: Dziuba Anna , Dzierżak Sylwia , Sodo Anna , Wawszczak-Kasza Monika , Zegadło Katarzyna , Białek Jakub , Zych Natalia , Kiebzak Wojciech , Matykiewicz Jarosław , Głuszek Stanisław , Adamus-Białek Wioletta 
Published: November 2023

IntroductionUrinary tract infections (UTI), among which the main etiological factor is uropathogenic Escherichia coli (UPEC, E. coli), remain an important issue for clinicians. The aim of the study was to demonstrate clear differences in the pathogenic properties of urine-derived E. coli compared to other extraintestinal E. coli clinical isolates (derived from: blood, lower respiratory tracts, sputum, reproductive tract, body fluids, perianal pus, other pus, wound, postoperative wound and other sources).MethodsThe collection of 784 E. coli isolates was collected from various materials of hospitalized patients. They were analyzed in terms of virulence-associated genes (papC, sfaD/sfaE, cnf1, usp., fimG/H, hlyA), belonging to phylogenetic groups and the presence of CRISPR-Cas regions using PCR. In addition, the epidemiological data and the antibiotic resistance profiles provided by the hospital’s microbiology department were included for statistical analyses.ResultsUrine-derived E. coli showed significantly greater virulence potential compared to other isolates, but they were generally unremarkable in terms of drug resistance. The isolates most often belonged to phylogenetic group B2. Drug resistance was negatively correlated with CRISPR 2 presence and high average virulence score, but positively correlated with CRISPR 4 presence. To the best of our knowledge, we are the first to report significant differences in sputum-derived isolates—they revealed the lowest virulence potential and, at the same time, the highest drug resistance.DiscussionIn conclusion, we demonstrated significant differences of urinary-derived E. coli compared to other clinical E. coli isolates. We would like to suggest excluding penicillins from use in E. coli infection at this time and monitoring strains with a high pathogenicity potential.
MicroRNAs as biomarkers for trastuzumab-based therapy in HER2-positive advanced oesophago-gastric cancer patients
Authors: Lote Hazel , Mousoullou Florentia , Vlachogiannis George , Lampis Andrea , Satchwell Laura , Peckitt Clare , Fong Caroline , Begum Ruwaida , Kidd Shannon , Cromarty Susan , Gordon Anderley , Fribbens Charlotte , Rao Sheela , Starling Naureen , Chau Ian , Cunningham David , Valeri Nicola 
Published: November 2023

BackgroundThis study aimed to identify microRNAs (miRs) as circulating biomarkers of resistance to first-line trastuzumab-based therapy in advanced HER2-positive oesophago-gastric cancer patients.MethodsA high-throughput 1015 Exiqon miRCURY LNA™ microRNA inhibitor library screen was performed in trastuzumab-treated HER2-positive NCI-N87 and HER2-negative FLO-1 oesophago-gastric cancer cell lines. NanoString nCounter® miR analysis was performed in NCI-N87, FLO-1, and MAGIC trial (ISRCTN93793971) formalin-fixed paraffin-embedded (FFPE) oesophago-gastric cancer patient samples. MiR-148a-3p copies in plasma samples were quantified using digital droplet polymerase chain reaction (ddPCR) from HER2-positive oesophago-gastric cancer patients treated with standard-of-care trastuzumab-based therapy within the FOrMAT (NCT02112357) and PLATFORM (NCT02678182) clinical trials. The primary endpoints were overall survival (OS) for plasma miR-148a-3p HIGH (&gt;median) versus LOW (≤median). The secondary endpoints were progression-free survival (PFS) and 3-month progression-free rates (PFRs) miR-148a-3p HIGH versus LOW. PLATFORM sensitivity analysis normalised miR-148a-3p (NmiR-148a-3p).ResultsThe inhibition of miR-148a-3p reduced NCI-N87 relative cell viability (&lt;0.6) and expression was high (&gt;242) in NCI-N87 and HER2-positive MAGIC trial patients (n=5). Normalised-miR-148a-3p (NmiR-148a-3p) LOW versus HIGH demonstrated a statistically significant difference in 3-month PFRs (n=23; OR, 0.11 [0.02–0.78]; p=0.027; aOR, 0.03 [0.001–0.71], p=0.029) but no difference in OS or PFS. There was no statistically significant relationship between miR-148-3p LOW versus HIGH for OS (PLATFORM, n=62; hazard ratio [HR], 0.98 [0.57–1.66]; p=0.933; FOrMAT, n=8; HR, 0.54 [0.13–2.31]; p=0.322), PFS (n=62; HR, 1.08 [0.65–1.81]; p=0.759; FOrMAT, n=8; HR, 1.26 [0.31–5.07]; p=0.714), or PFRs (PLATFORM, n=31; odds ratio [OR], 0.67 [0.2–2.8]; p=0.577).ConclusionNormalised miR-148a-3p may be a relevant biomarker for trastuzumab-based therapy in advanced HER2-positive oesophago-gastric cancer patients.
Social behavioral impairments in SYNGAP1-related intellectual disability
Authors: Naveed Hajer , McCormack Maria , Holder J. Lloyd 
Published: November 2023

IntroductionDevelopmental synaptopathies are neurodevelopmental disorders caused by genetic mutations disrupting the development and function of neuronal synapses.MethodsWe administered the validated Social Responsiveness Scale, Second Edition (SRS-2) to investigate the phenotypic presentation of social-behavioral impairments for the developmental synaptopathy—SYNGAP1-related Intellectual Disability (SYNGAP1-ID) (n = 32) compared with a phenotypically similar disorder Phelan-McDermid syndrome (PMD) (n = 27) and healthy controls (n = 43). A short form SRS-2 analysis (n = 85) was also conducted.ResultsBoth SYNGAP1-ID and PMD had significantly elevated total and subcategory T-scores, with no significant score differences between SYNGAP1-ID and PMD, consistent between the full and short form. Mild to severe deficiencies in reciprocal social behavior were found in 100% of PMD individuals and 87.1% of SYNGAP1-ID individuals. Surprisingly, a positive correlation between age and total score was discovered for SYNGAP1-ID participants and not found in individuals with PMD or healthy controls.DiscussionThe short form demonstrated greater utility for SYNGAP1-ID participants due to lower item-omission rates. In conclusion, significant impairment in reciprocal social behaviors is highly prevalent in SYNGAP1-ID.
Bone health in children with severe cerebral palsy
Authors: Barbier Vincent , Goeb Vincent , Gouron Richard , Fritot Severine , Mentaverri Romuald , Klein Céline 
Published: November 2023

AimTo describe bone health and associated factors in children with severe cerebral palsy.MethodIn a retrospective, single-centre study, we performed a comprehensive bone evaluation (including clinical, densitometric and bone biomarker assessments) of children with severe cerebral palsy.ResultsNone of the 19 included children had a normal BMCTBLH Z score, and only one had a BMDTBLH Z score greater than −2. Six children had a BMDLS Z score greater than −2. The bone biomarker data were suggestive of excessive bone remodelling. Levels of bone remodelling markers factors and densitometric variables were not significantly related. Age, weight and pubertal stage were significantly related to bone mass.DiscussionOur results highlights the insufficient increase in bone mass with age (probably due to excessive bone remodelling) and confirms the high prevalence of low bone mineral density in children with severe cerebral palsy. Possible preventive measures might include calcium + vitamin D supplementation and the systematic management of underweight and delayed puberty. Bone remodelling markers might be of value for follow-up.
The impact of teaching-research conflict on turnover intention: cross-level interaction effect of justice climate
Authors: Siqi Zhao , Hong Wang 
Published: November 2023

IntroductionResearch-based on the Job Demands-Resources theory (JD-R theory) has revealed a close relationship between teaching-research conflict and job burnout. However, there needs to be more investigation into the complex relationship between teaching-research conflict and turnover intentions from the perspective of this theory. To address these gaps, this study, grounded in the JD-R theory, explores the relationships among teaching-research conflict, career adaptability, justice climate, job burnout, and turnover intention.MethodsData collected through an online survey involving 858 Chinese university teachers, and the analysis utilized a Multilevel Structural Equation Model (MSEM) with Maximum Likelihood (ML) estimation.ResultsThe findings reveal that job burnout mediates the relationship between teaching-research conflict and turnover intention. Career adaptability plays a moderating role in the connection between job burnout and turnover intention. Furthermore, justice climate exhibits a cross-level interaction effect concerning the relationship between teaching-research conflict and turnover intention.DiscussionThese findings offer innovative strategies for mitigating and preventing faculty turnover intention.
Exploring positive teacher-student relationships: the synergy of teacher mindfulness and emotional intelligence
Authors: Wang Xue 
Published: November 2023

IntroductionThis study delves into the intricate synergy between teacher mindfulness and the quality of teacher-student relationships, with a specific and deliberate focus on the mediating influence of emotional intelligence. The way teachers engage with their students not only impacts the learning outcomes but also contributes significantly to the overall classroom atmosphere. Understanding the underlying mechanisms that drive these relationships is crucial for educators and policymakers alike. This research seeks to shed light on these critical dynamics.MethodsTo investigate this multifaceted interplay, a participant pool of 369 Chinese English teachers was assembled. The research employed a comprehensive approach to data collection, utilizing self-report questionnaires completed by the instructors. Structural equation modeling, a robust statistical technique, was employed to rigorously analyze the collected data.ResultsThe data analysis unveiled a robust and direct association between teacher mindfulness and the quality of teacher-student relationships. Beyond this primary link, a noteworthy revelation emerged: emotional intelligence, as measured through our analysis, was identified as a pivotal mediating factor in this relationship. This finding highlights the intricate web of emotions, awareness, and interpersonal interactions that underpin effective teaching and positive teacher-student relationships.DiscussionThese significant findings underscore the critical roles of teacher mindfulness and emotional intelligence in shaping the educational landscape. The implications of this study reach far beyond academia, extending to the development of tailored educational interventions and support strategies.
Knowledge and attitude toward depression among healthcare professionals working in Ilu Aba Bor zone, Oromia regional state, Ethiopia, 2021: a cross sectional study
Authors: Alemayehu Yadeta , Girma Million , Bacha Lemi , Boka Kefale , Tarafa Hunde 
Published: November 2023

BackgroundDepression is the major mental disorder that frequently co-occurs with other physical illnesses, although its detection at primary healthcare is limited. Thus, the purpose of this study is to evaluate health professionals’ knowledge and attitude toward depression and its related factors.ObjectiveTo assess knowledge and attitude of healthcare professionals toward people with depression at different health facilities of Ilu Aba Bor zone, Ethiopia, 2021.MethodsA cross-sectional study was carried out involving 404 primary healthcare professionals using a systematic random sampling technique in February 2021. Attitude was assessed using depression attitude questionnaires. Epi-data version 3.1 and SPSS version 26 was used for data entry and analysis. The p-values lower than 0.05 were deemed statistically significant.ResultIn the current study, 30.4% (95% CI; 25.86, 34.94) of the respondents have inadequate knowledge and 29.9% (95% CI; 25.4, 36.8) have negative attitude toward depression. A vast majority (77.7%) of healthcare professionals have never received any kind of training on mental health. Accordingly, contact with the person with mental illness and mental health training were significantly associated with inadequate knowledge. In addition, perceived cause of depression and mental health training were significantly associated with negative attitude.ConclusionAccording to this study, the magnitude of inadequate knowledge and negative attitude in the diagnosis and treatment of depression is comparably high. Therefore, primary healthcare settings should prioritize raising awareness, promoting positive attitudes, and improving detection and treatment of depression cases.
Can COVID-19 mark a tipping point for home-based telework? Conflict between untact technology and rigid institutions in Korea
Authors: Cho Joonmo , Lee Sanghee , Park Byungjin 
Published: November 2023

BackgroundPreviously, Korea showed a passive attitude toward home-based telework; however, this stance rapidly changed after the COVID-19 pandemic. Sustaining home-based telework entails adjusting productivity conditions, introducing performance-based evaluations, and modifying employment rules, as required by the Korean Labor Standards Act, which demand the consent of most workers. This study aims to explore the societal and institutional shifts necessary for ongoing home-based telework post-pandemic.MethodsThis study discusses the sustainability of home-based work based on survey data and materials from institutions and previous research. It used data from the Workplace Panel Survey provided by the Korea Labor Institution for 3 years (2015, 2017, and 2019) to examine the status of home-based work and business responses. It also addresses legal issues related to changes in working conditions and worker-management agreements resulting from telework implementation. Legal aspects of telework are explained using relevant sections of Korea’s labor laws.ResultsTo establish home-based telework as a working method relevant to the Fourth Industrial Revolution after the pandemic, essential discussions are needed regarding its fundamental applicability to specific job sectors. Moreover, to activate home-based telework without deteriorating working conditions, achieving agreement between workers and management is imperative. However, legal complexities necessitate systemic changes for effective resolution. For the sustainable continuity of telework, a blend of societal awareness and institutional transformations is indispensable.DiscussionThe growth of home-based telework through untact technology expansion is hindered by inflexible Korean labor laws, judicial precedents, and worker-management relations. The absence of necessary legal and organizational changes could lead Korea to revert to pre-pandemic norms or slow implementation. Initially prevalent in IT, home-based telework has expanded across sectors due to the pandemic. Leading the “new normal,” companies creatively enhance productivity through telework, but rigid systems and outdated cultures could impede post-pandemic progress.ConclusionThe study highlights the need for forward-looking institutional changes and adaptation to advancing technology. It provides valuable insights for organizations and policymakers to optimize work dynamics and enhance employee and employer well-being in the post-COVID-19 era.
Evaluation of a Method for Standardized Antimicrobial Susceptibility Testing with Mycoplasma hyorhinis Field Isolates
Authors: Käbisch Lisa , Schink Anne-Kathrin , Hoeltig Doris , Verspohl Jutta , Gyuranecz Miklós , Spergser Joachim , Kehrenberg Corinna , Schwarz Stefan 
Published: November 2023

Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for Mycoplasma spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of Mycoplasma hyorhinis using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of Mycoplasma hyorhinis isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future.