bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fractionation of Xanthium strumarium L. foliage phenolics, in-vitro antioxidant activities, and in-vivo anti-diabetic potential
Authors: Shaheen Asma , Akram Sumia , Sharif Saima , Rashid Ayoub , Adnan Ahmad , Mushtaq Muhammad 
Published: November 2023

Introduction: The present research aimed to fractionate Xanthium strumarium L. (XSL) foliage phenolics into a set of solvents and evaluate their antioxidant potential and in-vivo anti-diabetic activity against Alloxan monohydrate-induced diabetic mice.Methodology: For this purpose, XSL foliage was fractionated into petroleum ether, ethyl acetate, ethanol, and water via orbital type shaking and tested for the presence of phenolics, and their antioxidant and antidiabetic potential.Results and discussion: The results revealed that the ethyl acetate fraction of XSL foliage contained the highest amount of total phenolics 95.25 mg GAE/g of extract, followed by ethanol (65.14 mg GAE/g), petroleum ether (25.12 mg GAE/g), water (12.20 mg GAE/g), and XSL powder (69.13 mg GAE/g). At the end of treatment time (day 18 of oral administration of 400 mg/kg body weight of mice), the ethyl acetate fraction significantly (p ≤ 0.05) lowered blood glucose level (353 ± 10.6 to 220 ± 25.5 mg/dL) which might due to the elevated level of phenolic compounds in this fraction.Conclusion: Overall, it can be speculated that ethyl acetate and ethanol may work efficiently for the enrichment of XSL phenolic without compromising their antidiabetic potential.
Collective memories and previous experiences of older people in the face of disaster risk processes: lessons learned, implication, and social support
Authors: Sandoval-Díaz José , Navarrete-Valladares Camila , Suazo-Muñoz Consuelo , Martínez-Labrín Soledad 
Published: November 2023

IntroductionClimate change amplifies the vulnerability of various groups, especially of older people. Though seen as highly vulnerable, studies reveal their remarkable resilience and that they experience less distress than the young population. Collective memory and experience thus act as coping devices, facilitating learning processes and adaptation when faced with natural risks.MethodThe objective was to characterize the lessons learned, implications and social support perceived by the older people in disaster risk situations. The study was conducted with six groups of older people in Ñuble (Chile) using the SWOT matrix (Strengths, Weaknesses, Opportunities, and Threats), life stories, focus groups, photo evocation, and other participatory techniques. Content analysis was based on a systematic coding of category quantifications.ResultsNegative impacts and positive learning processes were identified that prompted significant changes and made post-traumatic growth processes and capacity-building possible. The type of disaster, its frequency and stage in which it occurs influence the perception of risk and coping strategies, underscoring the importance of strengthening communication about natural risks that are often rendered invisible, such as heat waves. Social support is the main source of collective capacity and has been shown to reduce perceived vulnerability to disaster risk scenarios.ConclusionsThe collective memory and prior disaster experiences of the older people provide them with tools to reinterpret new risk scenarios, highlighting their abilities and promoting their empowerment. These resources are crucial for the development of intergenerational learning aimed at comprehensive risk management. However, facing recent disaster risk situations such as COVID-19 or heatwaves has posed a challenge due to a lack of prior experiences and knowledge on how to handle them. This underscores the importance of having continuous psychoeducation, tailored to the specific and territorial needs of the older people, especially in the context of climate change.
Ezetimibe and atherosclerotic cardiovascular disease: a systematic review and meta-analysis
Authors: Omidi Fatemeh , Rahmannia Maryam , Shahidi Bonjar Amir Hashem , Mohammadsharifi Parsa , Nasiri Mohammad Javad , Sarmastzadeh Tala 
Published: November 2023

IntroductionIndividuals diagnosed with atherosclerotic cardiovascular disease (ACD) are exposed to an increased risk of cardiovascular events. Reducing low-density lipoprotein cholesterol (LDL-C) levels has been established as an effective approach to mitigate these risks. However, a comprehensive and up-to-date meta-analysis investigating the LDL-C-lowering effectiveness and the impact on coronary atherosclerotic plaque compositions of Ezetimibe has been lacking.MethodsWe conducted a systematic review by meticulously analyzing databases such as MEDLINE, EMBASE, and the Cochrane CENTRAL for randomized controlled trials that evaluated the efficacy of ezetimibe in lowering LDL-C levels and its influence on coronary atherosclerotic plaques among individuals with ACD. This review encompassed studies available until August 1, 2023. In our analysis, we employed the weighted mean difference (WMD) as the aggregated statistical measure, accompanied by the corresponding 95% confidence interval (CI).ResultsWe encompassed a total of 20 eligible studies. Our findings unveiled that the combined therapy involving ezetimibe alongside statins led to a more substantial absolute decrease in LDL-C in comparison to using statins alone. This difference in means amounted to (−14.06 mg/dl; 95% CI −18.0 to −10.0; p = 0.0001). Furthermore, upon conducting subgroup analyses, it became evident that the intervention strategies proved effective in diminishing the volume of dense calcification (DC) in contrast to the control group.ConclusionsOur study findings indicate that the inclusion of ezetimibe in conjunction with statin therapy leads to a modest yet meaningful additional reduction in LDL-C levels when compared to using statins in isolation. Importantly, the introduction of ezetimibe resulted in a significant decrease in the volume of DC. However, it is worth noting that further investigation is warranted to delve deeper into this phenomenon.
Regional character of geomagnetic field directional circularity: Holocene Eastern North America
Authors: Lund Steven P. 
Published: November 2023

This study characterizes the paleomagnetic secular variation (PSV) in five Holocene records from Eastern North America. We have determined equi-spaced (100-year) declination and inclination time series for the five sites and compared their directional variability. We note ten correlatable features in both inclination and declination, which indicate that these five PSV records all lie within the same PSV region. We focus on clockwise or counter-clockwise looping of paleomagnetic directions (termed circularity) in these PSV records. We have used two techniques to estimate the circularity. We have first calculated the rate and direction of motion in 200-year intervals for each record. We have also looked for discernable looping (full loops down to 1/2 loops) in individual records. We estimate the loop sizes, durations, and circularity direction. We note the same pattern of circularity in both methods. There are seven intervals of alternating circularity in all five sites. They normally agree in timing and direction of circularity. Both methods suggest an alternating, teeter-totter like, behavior to PSV circularity. This teeter-totter behavior is unbalanced with more time spent in counter-clockwise motion than in clockwise motion. The loops have a distribution of sizes and durations with smaller loops being shorter in duration (400–800 years) and bigger loops having longer durations (600–1,200 years). The teeter-totter alternation of clockwise versus counter-clockwise circularity is distinctive and may be a normal aspect of PSV. The alternation is not consistent with several traditional ideas about fluid flow (drift, whirling motion, simple convection) in the outer core. Recent studies have identified torsional oscillations as a source for both historical short-term (decadal) and millennial-scale (103) secular variation. Such a pattern of fluid flow might create the alternating teeter-totter-like behavior of circularity that we see. All five PSV records also display five short (&lt;400 years) intervals of higher-amplitude acceleration and faster PSV rates. These features are analogous to geomagnetic jerks in that they are short intervals of anomalous acceleration. Previous studies showed evidence in PSV for similar anomalous acceleration intervals (∼102 yrs). We think our anomalous intervals are comparable to them.
Enhanced trimethylamine metabolism and gut dysbiosis in type 2 diabetes mellitus with microalbumin
Authors: Huo Lixia , Li Hui , Zhu Ming , Liu Yang , Ren Lingyan , Hu Jia , Wang Xiaoyi 
Published: November 2023

BackgroundAbnormal gut microbiota and blood trimethylamine-N-oxide (TMAO) metabolome have been reported in patients with type 2 diabetes mellitus (T2DM) and advanced diabetic nephropathy. This study aimed to investigate the gut microbiota profiles and a group of targeted urine metabolic characteristics in T2DM patients with or without microalbuminuria, to determine the correlation between the gut microbiota composition, trimethylamine (TMA) metabolism, and the clinical features during progression of diabetic kidney disease (DKD)MethodsThis study included 26 T2DM patients with microalbuminuria (Micro), 26 T2DM patients with normoalbuminuria (Normo), and 15 healthy controls (HC). Urine and Fecal samples were detected using ultra performance liquid chromatography tandem mass spectrometry and 16S ribosomal DNA gene sequencing, respectively.ResultsThe TMAO/TMA ratio decreased gradually during the HC-Normo-Micro transition. The levels of TMA, choline and betaine were significantly different between the HC group and the T2DM patients belonging to both Normo and Micro groups. At the operational taxonomic unit (OTU) level, the gut microflora diversity was significantly reduced in the Micro groups compared to the HC groups and the Normo groups. Taxonomic analyses revealed significant consumption in the relative abundances of eight bacterial genera and significant enrichment of two bacterial genera during the HC-Normo-Micro transition. Furthermore, the relative abundances of six bacterial genera, namely, Ruminococcus_1, [Eubacterium]_ruminantium_group, Roseburia, Faecalibacterium, Fusicatenibacter and Coprococcus_3 exhibited significant differences, and were associated with elevated urinary albumin creatinine ratio (UACR), TMAO/TMA, TMA and its precursors in the Micro group compared with the other groups.ConclusionThe imbalance of gut microbiota has occurred in patients with early-stage DKD, and the consumption of short-chain fatty acid-producing bacteria were associated with the accumulation of TMA and UACR.
Genome-wide DNA methylation pattern in whole blood of patients with Hashimoto thyroiditis
Authors: Zhou Zheng , Liu Jinjin , Chen Yun , Ren Bingxuan , Wan Siyuan , Chen Yao , He Yanhong , Wei Qiuyang , Gao Haiyan , Liu Lixiang , Shen Hongmei 
Published: November 2023

BackgroundHashimoto thyroiditis (HT), a prevalent autoimmune disorder, is not yet thoroughly understood, especially when it comes to the influence of epigenetics in its pathogenesis. The primary goal of this research was to probe the DNAm profile across the genome in the whole blood derived from patients suffering from HT.MethodUsing the Illumina 850K BeadChip, we conducted a genome-wide DNAm assessment on 10 matched pairs of HT sufferers and healthy individuals. Genes with differential methylation (DMGs) were identified and underwent functional annotation via the databases of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. The transcriptional significance of potential epigenetic biomarker genes was corroborated through qRT-PCR.ResultsThe DNAm profiling across the genome indicated an overall reduction in methylation in HT subjects in comparison with their healthy counterparts. We detected 283 DMPs (adjusted P &lt; 0.05 and |Δβ| &gt; 0.1), among which 152 exhibited hypomethylation and 131 demonstrated hypermethylation. Further analysis exposed a noteworthy concentration of hypermethylated DMPs in the 3´UTR, North Shore, and CpG islands, while there was a significant decrease in the Open Sea (all P &lt; 0.001). The 283 DMPs were broadly distributed from chromosome 1 to 22, with chromosome 6 harboring the most DMPs (n = 51) and chromosome 12 carrying the most DMGs (n = 15). The SLFN12 gene, which presented with extreme hypomethylation in its promoter DMPs among HT patients, was identified as the epigenetic marker gene. Consequently, the SLFN12 mRNA expression was markedly upregulated in HT, displaying a negative relationship with its methylation levels. The area under curve (AUC) value for the SLFN12 gene among HT patients was 0.85 (sensitivity: 0.7, specificity: 0.7), a significant difference compared with healthy controls. The methylation levels of all DMPs in SLFN12 gene were negatively correlated with TSH and one CpG site (cg24470734) was positively assocciated with FT4.ConclusionThis investigation presents an initial comprehensive DNAm blueprint for individuals with HT, which permits clear differentiation between HT subjects and normal controls through an epigenetic lens. The SLFN12 gene plays a pivotal role in the onset of HT, suggesting that the methylation status of this gene could serve as a potential epigenetic indicator for HT.
Untargeted metabolomics reveals dynamic changes in metabolic profiles of rat supraspinatus tendon at three different time points after diabetes induction
Authors: Xu Kuishuai , Zhang Liang , Wang Tianrui , Ren Zhongkai , Yu Tengbo , Zhang Yingze , Zhao Xia 
Published: November 2023

ObjectiveTo investigate the dynamic changes of metabolite composition in rat supraspinatus tendons at different stages of diabetes by untargeted metabolomics analysis.MethodsA total of 80 Sprague–Dawley rats were randomly divided into normal (NG, n = 20) and type 2 diabetes mellitus groups (T2DM, n = 60) and subdivided into three groups according to the duration of diabetes: T2DM-4w, T2DM-12w, and T2DM-24w groups; the duration was calculated from the time point of T2DM rat model establishment. The three comparison groups were set up in this study, T2DM-4w group vs. NG, T2DM-12w group vs. T2DM-4w group, and T2DM-24w group vs. T2DM-12w group. The metabolite profiles of supraspinatus tendon were obtained using tandem mass spectrometry. Metabolomics multivariate statistics were used for metabolic data analysis and differential metabolite (DEM) determination. The intersection of the three comparison groups’ DEMs was defined as key metabolites that changed consistently in the supraspinatus tendon after diabetes induction; then, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed.ResultsT2DM-4w group vs. NG, T2DM-12w group vs. T2DM-4w group, and T2DM-24w group vs. T2DM-12w group detected 94 (86 up-regulated and 8 down-regulated), 36 (13 up-regulated and 23 down-regulated) and 86 (24 up-regulated and 62 down-regulated) DEMs, respectively. Seven key metabolites of sustained changes in the supraspinatus tendon following induction of diabetes include D-Lactic acid, xanthine, O-acetyl-L-carnitine, isoleucylproline, propoxycarbazone, uric acid, and cytidine, which are the first identified biomarkers of the supraspinatus tendon as it progresses through the course of diabetes. The results of KEGG pathway enrichment analysis showed that the main pathway of supraspinatus metabolism affected by diabetes (p &lt; 0.05) was purine metabolism. The results of the KEGG metabolic pathway vs. DEMs correlation network graph revealed that uric acid and xanthine play a role in more metabolic pathways.ConclusionUntargeted metabolomics revealed the dynamic changes of metabolite composition in rat supraspinatus tendons at different stages of diabetes, and the newly discovered seven metabolites, especially uric acid and xanthine, may provide novel research to elucidate the mechanism of diabetes-induced tendinopathy.
Prediction of natural fracture distribution characteristics in tight oil and gas reservoirs based on paleotectonic stress field
Authors: Lin Lifei , Wang Mingwei , Wang Yanwei , Gao Yi , Li Song 
Published: November 2023

The production of fractured oil and gas reservoirs in the world accounts for more than half of total oil and gas production and is one of the important fields for increasing oil and gas storage and production in the 21st century. The key to characterizing fractured oil and gas reservoirs is the distribution pattern of natural fractures. The distribution of natural fractures is dominated by structural deformations and fractures. Therefore, analytical and structural simulations of the tectonic stress field in geological structural systems play a crucial role in obtaining fracture distribution patterns. In this paper we have developed a description of the distribution of natural fractures based on finite element numerical simulations of the paleotectonic stress field. The study focused on the Chang 7 and Huangjialing Chang 8 reservoirs in the underground Siwan region of the Ordos Basin in China. First, an experimental assessment of the rock mechanics of the reservoir was completed, and the values of the paleotectonic stresses obtained from the tests were used as inversion criteria for the stress field simulations. Based on the geology, a refined geological model has been developed to study the structure of the Chang 7 and Chang 8 oil reservoirs in the block. The simulated paleotectonic stress fields for natural fractures in the Indosinian period are as follows: maximum principal stress of 94.67 MPa, minimum principal stress of 21.58 MPa, and vertical stress of 28.07 MPa. The direction of maximum principal stress remains essentially in the NE-SW direction, with the largest differential stress occurring in the Chang 8 oil layer group in Huangjialing, while the differential stress is relatively uniform in the Chang 7 oil layer group in Xiasiwan. It predicts the relative density of natural fractures in the Chang 7 and Chang 8 reservoirs, and finds high-to-low plane heterogeneity in the Huangjialing Chang 8 reservoir group and the Xiasiwan Chang 7 reservoir group, respectively. The paper reveals the pattern of development of reservoir fractures in both vertical and horizontal directions, providing an important geological basis for efficient and rational exploitation of oil and gas resources in the study area and improving oil recovery.
Prenatal genetic diagnosis associated with fetal ventricular septal defect: an assessment based on chromosomal microarray analysis and exome sequencing
Authors: Wang You , Li Ru , Fu Fang , Huang Ruibin , Li Dongzhi , Liao Can 
Published: November 2023

Objective: In the study, we investigated the genetic etiology of the ventricular septal defect (VSD) and comprehensively evaluated the diagnosis rate of prenatal chromosomal microarray analysis (CMA) and exome sequencing (ES) for VSD to provide evidence for genetic counseling.Methods: We carried out chromosomal microarray analysis (CMA) on 468 fetuses with VSD and exome sequencing (ES) on 51 fetuses.Results: In our cohort, 68 (14.5%) VSD fetuses received a genetic diagnosis, including 61 (13.03%, 61/468) cases with chromosomal abnormalities and seven (13.7%, 7/51) cases with gene sequence variants. The detection rate of total pathogenic and likely pathogenic gene variations in the non-isolated VSD group (61/335, 18.2%, 55 by QF-PCR/karyotype/CMA + 6 by ES) was significantly higher than that in the isolated VSD group (7/133, 5.3%, 6 by QF-PCR/karyotype/CMA + 1 by ES, p = 0.000). The most common copy number variation (CNV) was 22q11.2 microdeletion syndrome. Additionally, we found six previously unreported variants, which expanded the variation spectrum of VSD-related genes.Conclusion: In this study, CNVs and sequence variants were found in 13.03% and 13.7% of cases, respectively. ES can be recommended for fetuses with VSD without chromosome abnormalities and pathogenic CNVs, especially those that are combined with other ultrasound abnormalities.
Hippocampal microstructure, but not macrostructure, mediates age differences in episodic memory
Authors: Ibrahim Kirolos , Bennett Ilana J. 
Published: November 2023

IntroductionSeparate unimodal magnetic resonance imaging (MRI) literatures have shown that hippocampal gray matter macrostructure (volume) and microstructure (diffusion) decline with age and relate to episodic memory performance, with multimodal MRI studies reporting that episodic memory may be better explained by a combination of these metrics. However, these effects are often assessed independent of age or only within older adults and therefore do not address whether these distinct modalities explain variance in (i.e, mediate) the effect of age on episodic memory.MethodsHere, we simultaneously examined the unique and joint contribution of hippocampal volume and diffusion to age-related differences in episodic memory in 83 younger and 61 older adults who underwent a T1- and diffusion-weighted MRI and completed the Rey Auditory Verbal Learning Test.ResultsAs expected, older age was significantly related to smaller volume and higher diffusion (intracellular, dispersion, and free) in bilateral hippocampus and to worse episodic memory performance (immediate and delayed free recall, recognition). Structural equation modelling revealed that the age-memory relationship was significantly mediated by hippocampal diffusion, but not volume. A non-significant influential indirect effect further revealed that the structural metrics did not jointly mediate the age-memory relationship.DiscussionTogether, these findings indicate that hippocampal microstructure uniquely contributes to age-related differences in episodic memory and suggest that volume and diffusion capture distinct neurobiological properties of hippocampal gray matter.
Short-term effects of etifoxine on human gut microbiome in healthy men
Authors: Manook André , Baghai Thomas C. , Riebel Marco , Nothdurfter Caroline , Schwarzbach Jens Volkmar , Gessner André , Rupprecht Rainer , Hiergeist Andreas 
Published: November 2023

BackgroundNeurosteroids have recently gained in interest as a treatment strategy for affective disorders. Etifoxine is known for its dual mode of action, one of which is to stimulate endogenous neurosteroid synthesis. The gut microbiome has been studied in affective disorders, but it has not been investigated in the context of human etifoxine or neurosteroid interventions.MethodsWe performed a crossover study with 36 healthy male volunteers who received etifoxine versus alprazolam and placebo in a balanced Williams design. Participants were randomized into six sequences and went through three 5-day treatments followed by wash-out phases of 9 days. Bacterial compositions in stool samples were determined by high-throughput 16S rRNA amplicon sequencing.ResultsGut microbiome analyses revealed no relevant effects between treatments with respect to alpha and beta diversity. Differential abundance analyses yielded etifoxine treatment as the only effect related to changes in microbial features with reductions of Faecalibacterium duncaniae, Roseburia hominis and Lactobacillus rogosae (i.e., Bacteroides galacturonicus).ConclusionHere we report on the first human investigation of the gut microbiome with short-term etifoxine intervention. Differences in diversity and compositional structure of the microbiome were more likely due to between- subject effects rather than medication. However, five-day treatment with etifoxine reduced the abundance of a few bacterial species. These species are currently seen as beneficial components of a healthy intestinal microbiome. This reduction in abundances may be related to elevated endogenous neurosteroids.