bluuebunny/crossref_metadata_2025_split huggingface dataset:
TINGKAT KONDISI FISIK PESERTA EKSTRAKURIKULER FUTSAL PUTRA DI MAN 4 BANJAR
Authors: Suhendra Yuyu , Amirudin Akhmad , Mulhim Muhammad 
Published: December 2023

Penelitian ini bertujuan untuk mengetahui bagaimana tingkat kondisi fisik peserta ekstrakurikuler futsal putra di MAN 4 Banjar. Metode penelitian dengan menggunakan metode penelitian deskriptif kuantitatif, dengan teknik pengambilan data observasi, tes dan pengukuran. Populasi dalam penelitian ini adalah peserta ekstrakurikuler futsal MAN 4 Banjar yang berjumlah 30 peserta didik, Pengambilan sampel dilakukan dengan teknik purposive sampling dengan syarat yang telah ditentukan oleh peneliti yaitu keaktifan dan bersedia untuk di teliti. dengan jumlah sampel 15 peserta didik yang merupakan tim inti dari ekstrakurikuler futsal MAN 4 Banjar. Analisis data yang digunakan adalah analisis statistika deskriptif dengan metode persentase. Dari hasil penelitian Hasil penelitian yang sudah dilakukan tes dan pengukuran terhadap tim inti dari ekstrakurikuler futsal MAN 4 Banjar sebagai berikut; (1) Kecepatan di peroleh pada kategori baik 5 peserta didik dengan persentase 33,3% pada kategori cukup 5 peserta didik dengan persentase 33,3%, pada kategori kurang 5 peserta didik dengan persentase 33,3%. (2) Kelincahan diperoleh pada kategori cukup 3 peserta didik dengan persentase 20%, dan pada kategori kurang sekali 12 peserta didik dengan persentase 93%. (3) Daya ledak otot tungkai diperoleh 10 peserta didik dengan persentase 66,6% pada kategori baik, 3 peserta didik dengan persentase 20% pada kategori kurang 1 peserta didik dengan persentase 6,6%, dan pada kategori kurang sekali 1 peserta didik dengan persentase 6,6%. (4) Daya tahan kardiorespirasi diperoleh 1 peserta didik kategori cukup dengan persentase 6,6%, dan pada kategori kurang 14 peserta didik dengan persentase 93,3%. Kesimpulan dari penelitian tentang tingkat kondisi fisik peserta ekstrakurikuler futsal putra di MAN 4 Banjar sebagian besar dominan berada pada kategori kurang.
Family income does not necessarily lead to a holistic e-learning experience during COVID-19: A study in Sri Lanka
Authors: Thilakasiri Isuru , Senarath Neranjan , Dedunupitiya Wathsala , Ilangarathna Gayanthi , Samarakoon Bhagya , Ramanayake Lakshitha , Godaliyadda Roshan , Ekanayake Parakrama , Herath Vijitha , Pinnawala Mallika , Yatigammana Sakunthala , Ekanayake Janaka , Dharmarathne Samath , Tilakaratne Ganga 
Published: December 2023

Abstract

The COVID-19 outbreak caused a disruption of the conventional education system worldwide. This resulted in a shift towards online mode of delivery. This study analyzed the pandemic's effect on the education of students from diverse socioeconomic groups in Sri Lanka. The required data for this study was collected through a nation-wide field survey, covering 3020 households. The factor analysis (FA) performed identified seven significant factors. Thereafter, K-means and spectral clustering were used to cluster the population in order to identify the socio-economic groups that were impacted in a similar manner. This resulted in the identification of three clusters whose demographics and relationship to the extracted factors were extensively analyzed and interpreted. The study concluded that students in high-income households were more emotionally affected, whereas students in low-income households were mostly affected by a lack of resources. The study also concluded that students from mid-income households were the least affected emotionally.
‘BLUES’ procedure for assessing the blue level of the sclera in Osteogenesis Imperfecta
Authors: Martino Valerio Di , Mallone Fabiana , Lambiase Alessandro , Celli Mauro , Mannocci Alice , Celli Luca , Mangiantini Pietro , Fino Pasquale , Moramarco Antonietta 
Published: December 2023

Abstract

Purpose: Blue sclera is a characteristic and common clinical sign of Osteogenesis Imperfecta (OI). However, there is currently no widely accepted, objective method for assessing and grading blue sclera in individuals with OI.
To address this medical need, this study is aimed to design and validate a new method called ‘BLUES’ (BLUe Eye Sclera) to objectively identify and quantify the blue color in the sclera of patients affected by OI.
Methods: Sixty-two patients affected by OI and 35 healthy controls were enrolled in the present prospective study, for a total of 194 eyes analyzed.
In the 'BLUES' procedure, eye images from patients with OI and control subjects were analyzed to assess and grade the blue level of the sclera using Adobe Photoshop Software. The validation process then involved comparing the results obtained with the ‘BLUES’ procedure to the judgement of experienced ophthalmologists (JEO).
A receiver-operating characteristic (ROC) curve analysis was used to examine the overall discriminatory power. The sensitivity and specificity levels and the Cohen's Kappa (K) indexes of ‘BLUES’ and ‘JEO’ were estimated versus the standard OI diagnosis. The K indexes of ‘BLUES’ versus ‘JEO’ were also evaluated.
Results. The optimal cut-off point of the scleral blue peak was calculated at 17%. Our findings demonstrated a sensitivity of 89% (CI95%: 0.835-0.945) and specificity of 87% (CI95%: 0.791-0.949) for the ‘BLUES’ procedure with an agreement versus the diagnosis of OI of 0.747. In comparison, the sensitivity and specificity of ‘JEO’ ranged from 89% to 94% and 77% to 100%, respectively, with an agreement ranging from 0.663 to 0.871 with the diagnosis of OI. The agreement between ‘BLUES ‘and ‘JEO’ evaluations ranged from 0.613 to 0.734.
Conclusions: Our findings demonstrated an 89% sensitivity and an impressive 87% specificity of our method to analyze the blue sclera in OI. The results indicated high agreement with disease diagnosis and were consistent with evaluations by experienced ophthalmologists. The ‘BLUES’ procedure appears to be a simple, reliable and objective method for effectively identify and quantify the blue color of the sclera in OI.
Sexual Risk Behaviors and HIV Incidence Among Men Who Have Sex with Men in Southern China: Investigating the Role of HIV Serostatus Disclosure
Authors: Mo Jinli , Tian Weiyi , Wang Min , Zhang Yun , Liang Yinxia , Peng Hongbin , Luo Xia , Huang Haimei , Tan Sumin , Zhu Jiawen , Cen Ping , Lan Guanghua , Wang Hao , Jiang Li , Ning Chuanyi 
Published: December 2023

Abstract
Background The impact of HIV serostatus disclosure on sexual risk behaviors and HIV risk among men who have sex with men (MSM) is unclear. We aim to examine associations between receiving HIV serostatus disclosure from partners before sex and sexual risk behaviors and reveal the impact of such disclosure on HIV incidence.
Methods We recruited HIV-negative MSM who had reported engaging in homosexual encounters within the previous 6 months. Participants completed questionnaires every 3 months and were tracked for 12 months. Generalized Estimation Equations (GEE) were used to evaluate factors associated with receiving HIV serostatus disclosure from partners and to examine the impact of receiving this disclosure on sexual risk behaviors.
Results Among 363 participants, 350 completed at least one follow-up. On average, 50.60% (95% CI: 47.30%-53.70%) of participants received HIV serostatus disclosure from sexual partnersduring the follow-up visits.The HIV incidence was 1.47 per 100 person-years (95% CI: 0.479-3.398). Participants who had tested for HIV in the last 3 months (aOR=3.145, 95% CI: 2.109-4.691; P＜0.001) were more likely to receive HIV serostatus disclosure from partners. Conversely, Participants who have male casual partners (aOR=0.209, 95% CI: 0.127-0.346, P＜0.001) were less likely to receive such disclosure.Adjusting for potential confounders in GEE models, receiving HIV serostatus disclosure from partners is not a contributing factor to multiple partners (aOR=0.468, 95% CI: 0.347-0.632, P＜0.001). There was no significant association between receiving HIV serostatus disclosure and engaging in unprotected anal intercourse (OR=1.128, 95% CI: 0.876-1.453, P=0.351). MSM who did not consistently receive HIV serostatus disclosure from their partners appeared to have a higher seroconversion rate than those who consistently received such disclosure, though the difference was not statistically significant (IRR=0.531; 95%CI=0.060-4.699, P=1.000).
Conclusion Our study found that the proportion of MSM who received HIV serostatus disclosure from their partners before sex was relatively low. HIV testing facilitates HIV serostatus disclosure. Receiving HIV serostatus disclosure from partners before sex does not increase risky sex. These findings promote regular HIV testing and encourage HIV serostatus disclosure with sexual partners.
An endangered native species is being replaced by hybrids: the new normal for primate conservation in the Brazilian Atlantic Forest
Authors: Guimarães-Lopes Vanessa , Loureiro Natasha Grosch , Damo Júlia Simões , de Melo Fabiano Rodrigues , Rodrigues Flávio Henrique Guimarães , Massara Rodrigo Lima 
Published: December 2023

Abstract
Biological invasions can interfere decisively with the dynamics of important ecological processes.For primates of the genus Callithrix (marmosets), congener introductions and hybridization represent one of the greatest threats to native species. The Rio Doce State Park (RDSP), in southeastern Brazil, has records of three species of Callithrix, the endangered native species C. aurita, and two species of introduced marmosets, C. geoffroyi and C. penicillata.We used the playback method combined with adapted N-mixture models to estimate abundance and detection probability for Callithrix spp. in relation to biological variables. We estimated a total of 139 individuals, equivalent to 0.12 ind./ha, all being hybrids, except for one individual of C. aurita observed in a group of hybrids.The abundance of Callithrix spp. was higher in locations close to the forest edge, possibly due to the high availability of insects and greater availability of shelters in these areas.The detection probability of Callithrix spp. correlated positively with precipitation, indicating that greater humidity, as well as greater availability of arthropods, may favor the detection of individuals due to their high foraging activity during these periods. The results of this study show the critical scenario of biological invasions in RDSP, with serious threats to the native species.We suggest urgent and integrated measures to contribute to the conservation of C. aurita, such as a management plan for the few individuals of the native species. The unprecedented adaptation of the N-mixture model showed effectiveness for estimate abundance and detection probability of acoustically-responsive primates such as species of the genus Callithrix.
Assessment of physicochemical properties among petroleum contaminated and normal soil: A multivariate statistical analysis
Authors: Mangla Himanshi , Dave Saurabh , Jain Smita , Pathak Hardik 
Published: December 2023

Abstract
Soil surveying and mapping are vital operations in soil science. They contribute to the evaluation of changes in soil quality between various geographical regions. This study intended to investigate the effect of geographic variability on soil properties gathered from different geographical areas of the Rajasthan state of north-western India, using conventional analytical methods. Normal and petroleum-contaminated soils were collected from four diversified regions of Rajasthan- namely, hyper saline wetland (Sambhar), arid desert region (Jaisalmer), semi-arid region (Ganganagar), and humid irrigated plains (Banswara). The correlations between normal and contaminated soil for these four stations were investigated using multivariate analyses, principal component analysis (PCA), and statistical quality control. The one-way ANOVA test is applied to determine the statistical significance between different sampling stations for both normal and contaminated soil. Since the p-value in both cases is less than 0.05, it reflects a significant difference in physical properties among all four stations. The PCA analysis suggests that the first three components have eigenvalues greater than one that can describe 100% of the total variability in soil properties. Heavy metals (Lead, Nickel, and Copper) are the primary measures of variability between sample points for contaminated soil. In contrast, physicochemical properties (pH, Conductivity, and Sodium) are the primary measures of variability between sample sites for normal soil.
Molecular Characterization and Expression Analysis of NS3 and NS4 Genes of Dengue Virus Serotype-2 from Pakistani Isolates
Authors: Batool Anum , Kanwal Nida , Akram Madiha , Rauf Sobiah , Navid Muhammad Tariq , Masood Furqan , Ali Liaqat 
Published: December 2023

Abstract

Background Dengue virus (DENV) infection poses a significant public health threat, particularly in tropical and subtropical regions. Current estimates suggest that approximately 390 million cases of dengue occur annually, putting around 3.97 billion people at risk of contracting the infection. Despite global efforts, no antivirals or preventive vaccines are presently available in the market for the clinical treatment of dengue infection due to its ill-defined pathogenesis mechanism and lack of suitablein vitroandininfection models. The aim of this study was to identify the prevalent DENV serotypes circulating in Pakistan and to characterize and express the non-structural genes (NS3 and NS4) of dengue virus to better understand the disease pathogenesis. Methods In the present study, we tested NS1 positive serum samples for the identification of dengue serotypes through nested PCR using serotype-specific primers. The viral RNA was purified from the serum samples and complementary DNA (cDNA) was synthesized. We targeted serotype-2 samples for the amplification of NS3 and NS4A genes through nested PCR using two sets of gene-specific primers. The PCR products were initially verified using gel electrophoresis and subsequently confirmed through Sanger sequencing. Further, we cloned the amplified NS3 and NS4A gene fragments in the pCR 2.1 cloning vector and expressed them in mammalian vector (pET28) for further functional analysis. Results The NS1-positive DENV samples were tested for various dengue serotypes, confirming that serotype-2 continues to be predominant in Pakistan after COVID-19 pandemic. We successfully amplified the NS3 and NS4A genes of dengue virus. The computational analysis revealed that NS3 and NS4A gene sequences were closely related to the DENV-2 strains isolated from other areas of Pakistan like Swat. This analysis also confirmed their homology above 98% with Indian and Saudi Arabian isolates. The amplified NS3 and NS4A genes were then cloned in pCR 2.1 cloning vector and subcloned in mammalian expression vector pET28. Further, the NS3 gene was transfected into mammalian cell line and tests were conducted with siRNA targeting the NS3 protein, resulting in approximately a 50% inhibition. Conclusions Our current study has confirmed the prevalence of DENV serotype-2 following the COVID-19 pandemic and provided a molecular characterization of the genetic landscape of the non-structural genes (NS3 and NS4). This investigation serves as an important underpinning for a comprehensive examination of the DENV non-structural genes, proposing valuable insights that can contribute to the development of early diagnosis and antiviral therapies against DENV infections.
28-day repeated oral administration study of mechanically fibrillated cellulose nanofibers (fib-CNF) according to OECD TG407
Authors: Yamashita Yoshihiro , Tokunaga Akinori , Aoki Koji , Ishizuka Tamotsu , Fujita Satoshi , Tanoue Shuichi 
Published: December 2023

Abstract

It is still unclear whether oral administration of mechanically fibrillated cellulose nanofibers (hereafter, fib-CNF), which is widely used among nanofibers, causes toxicity or, on the contrary, improves health, despite the reported safety and even beneficial effects of oral intake of chitin-based nanofibers. Therefore, it is crucial to adhere to the guidelines outlined in OECD TG407 for the evaluation of oral toxicity. This study aimed to assess the safety of orally administered fib-CNF through an acute toxicity study in rats in accordance with the OECD guidelines laid out in TG407 for four weeks. CNF "BiNFi-s" FMa-10005, obtained through the mechanical defibrillation of pulp-derived cellulose, was administered via forced oral administration to male and female Crl:CD(SD) rats at doses of 50, 150, 500, and 1000 mg/kg/day for a period of 28 days in a control group (vehicle: water for injection). The study aimed to assess the toxic effects of repeated administration, and the rats were also monitored for 14 days after the administration to evaluate the recovery from any toxic effects. Results indicate that no deaths occurred in either sex during the administration period, and no toxicological effects related to the test substance were observed in various assessments, including general condition and behavioral function observations, urinalysis, hematological examination, blood biochemical examination, necropsy findings, organ weights, and histopathological examination. Notably, only female rats treated with 1000 mg/kg/day of CNF exhibited a consistent reduction in body weight during the 14-day recovery period after the end of treatment. They also showed a slight decrease in pituitary and liver weights. However, hematological and blood biochemical tests did not reveal significant differences, suggesting a potential weight-suppressive effect of CNF ingestion.
Factors Influencing Surgery After Two Days of Hospitalization and Outcomes in Hip Fractures: using a National Administrative Database
Authors: SHIN Donggyo , Tandi Tinyami Erick , KIM SANG MI 
Published: December 2023

Abstract

The increasing number of surgeries following hip fractures is increasing medical costs globally. Prompt surgical interventions in hip fractures is to achieve the most favorable outcomes and minimizes the risk of complications. A retrospective review and analyzes using the Korean National Hospital Discharge In-depth Injury Survey database was used to identify the distribution within 2 days of hospitalization, the factors of after 2 days’ surgery in hip fractures, defined as patients having an injury code with a diagnosis of hip fracture and a hip fracture-related operation during 2015–2019. The proportion of patients in the two groups were compared using the chi-square test. In the analysis of factors associated with late surgery, multiple logistic regression analysis was performed. The mean hospital stay for hip fracture patients before surgery was 3.58 days with 40.6% of patients having surgery within 2 days of hospitalization. When multiple logistic regression was performed, male (OR 1.190), Medicaid beneficiary (OR 1.385), higher comorbidity (OR 1.365, OR1.612), weekends admission (2.384), Outpatient Hospitalization status (1.298), ORIF (0.823) were reasons for the late surgery. As the operation was delayed, the total length of stay (OR 1.028) and the length of stay after stay (OR 1.005) were extended. Late surgery did not affect death and complications in the hospital. However, the entire hospital stay of the patient and postoperative hospital stay were extended. Therefore, providers should establish a system for surgery as soon as possible after hospitalization of hip fracture patients and support measures.
Periocular Skin 'Tattooing' Caused by Neutral Pen Pigment Mistaken for Periorbital Bruising: A Case of Missed Diagnosis of Traumatic Intraorbital Pen Core Foreign Body
Authors: Xiong Chao , Ren Zhangjun , Li Xuemei , Chen Yunxiu , Li Yue , Yu Jinhai , Jin Qi , sang Zexi , Wang Siyi , Wang Anan , Gan Puying , Xu Qihua , Wang Yaohua , Liao Hongfei 
Published: December 2023

Abstract
Background When an eye trauma occurs, orbital foreign bodies are widespread. The most common types of foreign bodies are metal, followed by plant foreign bodies, while oil foreign bodies are rare. Here, we report a case of misdiagnosis of periorbital bruising caused by the tip of a blue gel pen core. It can easily be misinterpreted as subcutaneous congestion since it causes the skin to become blue, which can result in the orbital foreign bodies being ignored.Case presentation The child was stabbed by a gel pen at the upper eyelid of his left eye while playing, resulting in a skin tear under the eyebrow arch, and the surrounding skin was cyan. Because the wound was small and closed smoothly, only local cleaning and disinfection were given without suturing. Within one month after injury, the cyanosis of the skin did not subside, which was considered by the doctors as subcutaneous congestion after injury.Conclusions Our report for the blue gel pen orbital foreign bodies can provide some reference for the diagnosis and treatment experience. The reason for the missed diagnosis in this case was that the blue color of the skin in the wound area was mistaken for "bruise". Orbital foreign body impact injury must be thoroughly found all foreign body fragments, if can not be found should be highly suspected foreign body into the body. The wound tract of ocular trauma may be concealed. If conditions permit, it is recommended to perform imaging examination to avoid omission of foreign bodies.
The Development Status of Entrustable Professional Activities-Based Curricula in Orthopedic and Neurosurgical Spinal Surgery: A Systematic Review
Authors: Domann Maximilian , Bertram Fabiana , Fischer Martin R. , Stadler Matthias 
Published: December 2023

Abstract
Background: Postgraduate medical specialists´curricula often lack clearly defined and assessable outcomes. Entrustable Professional Activities (EPAs) emerge as a promising teaching concept aiming at competency-based training. This scoping review explores the current status of EPA-based curricula in orthopedic and neurosurgical spinal surgery and seeks to underscore the potential of EPAs in addressing the contemporary challenges in medical education and professional training in spinal surgery.Methods: A systematic review was executed employing two research methods: a literature review using the PubMed database with specified search terms related to EPAs in spinal surgery, and an examination of various professional societies' publications and direct inquiries regarding EPAs in spinal surgery.Results: The PubMed search yielded only two papers relevant to spinal surgery EPAs, with one identifying four EPAs in orthopedics and another presenting one explicit EPA in neurosurgery. Among the nine professional societies explored, only the AO Foundation, AO Spine, had defined EPAs for spinal surgery. The lack of substantial results underscores a noticeable gap in the research and application of EPAs within spinal surgery curricula.Conclusions: The sparse literature and limited engagement from professional societies reveal a substantial research gap in EPA-based curricula in spinal surgery, despite the promising potential of EPAs in enhancing medical education and professional training. This scoping review lays a foundation for future research, urging a collaborative effort among professional societies and educational stakeholders to develop and implement EPA-based curricula in spinal surgery, aiming at a more efficient, competency-centered professional training paradigm.