bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD27 exhibits clinical diagnostic value and reveals the immune heterogeneity as well as predicts clinical drug responses in hashimoto's thyroiditis
Authors: Dong YanMing , Bao Guoqiang 
Published: October 2023

Abstract

Hashimoto's thyroiditis (HT), recognized as chronic lymphocytic thyroiditis, is a widespread autoimmune malady predominantly impacting females in the youthful and middle-age demographics. Initially, HT can present as hyperthyroidism, and as the disease progresses, it may transition to hypothyroidism. Studies have indicated that HT pathogenesis involves multiple factors, comprising genetic predisposition, environmental triggers, and autoimmune processes. However, the exact mechanism underlying the development of HT remains unclear. Within the framework of our study, we aimed to identify key genes and potential molecular mechanisms underlying HT, with the objective of offering fresh insights into diagnostic and therapeutic targets for this condition. To accomplish this, we amalgamated datasets GSE138198 and GSE54958 as our training cohorts, and subsequently conducted analyses encompassing the identification of differentially expressed genes (DEGs) and weighted gene co-expression network analysis (WGCNA) to pinpoint the DEGs most intricately associated with HT. Subsequently, we employed protein-protein interaction (PPI) network analysis to ascertain hub genes. We then proceeded to validate the diagnostic efficacy of these hub genes through the utilization of receiver operating characteristic (ROC) curves and nomograms. Finally, CD27 was selected as the key genes in HT and validated using clinical samples. More importantly, in order to delve deeper into the role and potential mechanisms associated with CD27 in HT, we conducted a comprehensive array of analyses including GO, KEGG and GSEA enrichment analysis, and HT-infiltrating immune cells (HTICs) components as well as functions were performed between high CD27 and low-CD27 cohorts. The findings revealed that CD27 is highly expressed in HT tissues and has important clinical application value for HT. The CD27 expression in high-cohort exhibited a more pronounced enrichment in immune-related biological processes compared to the low-expression cohort. Furthermore, we conducted CIBERSORT analysis to assess the proportion of HTICs, revealing a strong association between several activated HTICs and CD27 expression. This implies that CD27 has the potential to serve as an indicator of the immune status in individuals with Hashimoto's thyroiditis. Consequently, CD27 plays a significant role in predicting clinical outcomes in HT patients, encompassing the overall condition of HT patients and their response to immunotherapy. Furthermore, CD27 holds promise as a valuable biomarker for reshaping the microenvironment in Hashimoto's thyroiditis. This suggests potential underlying mechanisms contributing to the pathogenesis and progression of HT. Such insights may offer novel perspectives for HT treatment, ultimately leading to enhanced therapeutic strategies and improved patient care.
Design and validation of an instrument to evaluate Person-Centered Care in Health Services.
Authors: Lehmann-Mendoza Rodolfo , Sarabia-González Odet , Figuerola-Escoto Rosa Paola , Luna David , Cortés-Moreno Gabriela Yanet , González-Mundo Ilicia , Caselín-Ledezma Rubén , Vázquez-Dávila Roberto Arturo , Martínez-Arredondo Hilario Ascención 
Published: October 2023

Abstract

Background The concept of quality in health care has evolved, placing greater importance on the patient's needs, culture, and social context, as well as their participation in clinical decision-making, as highlighted by Mead and Bower's Person-Centered Care Model. The aim of the present study was to design and validate an instrument to assess the extent to which healthcare services provided by PEMEX (Petróleos Mexicanos) offer person-centered care according to user perceptions. Methods The first phase comprised the development of 57 items based on the analysis of responses from an open-ended questionnaire administered to 30 users of Pemex healthcare services. This questionnaire was designed considering the four factors of the person-centered care model. Subsequently, a validation process was performed, including a sample of 330 individuals, with an exploratory factorial analysis to identify the initial instrument structure. Afterward, a confirmatory factorial analysis was carried out with 335 participants. Finally, a new confirmatory factorial analysis included 130 participants due to the refinements made in the previous phase. Internal consistency was assessed using Cronbach's α and McDonald's ω at every phase. Results The exploratory factor analysis retained 35 items in a single factor that accounted for 49% of the variance with an internal consistency of Cronbach's α and McDonald's ω = 0.97. Because the factorial structure by confirmatory factorial analysis was unsatisfactory, the initial model was refined, leading to the retention of 11 items with an internal consistency of Cronbach's α and McDonald's ω = 0.93. Due to the refinements, a new confirmatory factorial analysis was conducted with suitable goodness-of-fit criteria in most items, resulting in a Cronbach's α and McDonald's ω = 0.98. Conclusions The instrument exhibits suitable psychometric properties to be employed to measure the degree to which medical care is patient centered. The use of this instrument represents a strategy for the promotion of an innovative healthcare model.
The Association between Glymphatic System and Perivascular Macrophages in Brain Waste Clearance
Authors: Kaur Jasleen , Boyd Edward , Ding Guangliang , Zhang Li , Luo Hao , Li Qingjiang , Li Lian , Wei Min , Landschoot-Ward Julie , Chopp Michael , Zhang Zhenggang , Jiang Quan 
Published: October 2023

Abstract

The glymphatic system suggests the convective bulk flow of cerebrospinal fluid (CSF) through perivascular spaces and the interstitial spaces of the brain parenchyma for the rapid removal of toxic waste solutes from the brain. However, the presence of convective bulk flow within the brain interstitial spaces is still under debate. We first addressed this argument to determine the involvement of the glymphatic system in brain waste clearance utilizing contrast-enhanced 3D T1-weighted imaging (T1WI), diffusion tensor imaging (DTI), and confocal microscopy imaging. Furthermore, perivascular macrophages (PVMs), which are immune cells located within perivascular spaces, have not been thoroughly explored for their association with the glymphatic system. Therefore, we investigated tracer uptake by PVMs in the perivascular spaces of both the arteries/arterioles and veins/venules and the potential association of PVMs in assisting the glymphatic system for interstitial waste clearance. Our findings demonstrated that both convective bulk flow and diffusion are responsible for the clearance of interstitial waste solutes from the brain parenchyma. Furthermore, our results suggested that PVMs play an important function in glymphatic system-mediated interstitial waste clearance. The glymphatic system and PVMs could be targeted to enhance interstitial waste clearance in patients with waste-associated neurological conditions and aging.
QUANTUM KEY DISTRIBUTION FOR DETECTING EAVESDROPPER USING Ekert91 PROTOCOL
Authors: Kumari Rachna , Kumar Sanjay 
Published: October 2023

Abstract
The discipline of cryptography offers the means to shield confidential data from outsiders. Due to the emergence of parallel and high performance computers, conventional encryp- tion, whose security frequently depends on unproven computa- tional assumptions, can be easily broken. Quantum cryptography (QKD), it is based on an essential quantum mechanical principle, guarantees complete safety. In this paper, a web-based simulation for determining the presence of eavesdroppers in a quantum channel utilizing the Ekert91 protocol is designed using the Quantum Information Toolkit (QIT).
Implementation of a standardized lung ultrasound protocol for respiratory distress in a neonatal intensive care unit: an observational study
Authors: Firszt Oliver , Grabowska Agata , Saran Anna , Kluczewska Ewa 
Published: October 2023

Abstract
Objective: To assess the feasibility of implementing a simple point-of-care lung ultrasound (POC-LUS) evaluation and reporting protocol in a neonatal intensive care unit (NICU) and its effect on patient management.
Study design: Retrospective observational study of POC-LUS examinations performed in a level III NICU. Each examination was performed according to a standardized protocol. An independent radiologist-assessed chest X-ray (CXR) was used to compare the POC-LUS diagnosis. The impact on patient management was also evaluated.
Result: A total of 206 POC-LUS studies in 158 neonates were reviewed. There was significant agreement between POC-LUS and CXR diagnoses (84,95%, 95% CI 80,07-89,83%).
POC-LUS affected patient management in 87.8% of the cases (95% CI 83,33-92,28%).
Conclusion: Implementation of a simplified, sign-based protocol for POC-LUS in the NICU is feasible. POC-LUS is not inferior to CXR studies and supports patient management as an imaging modality.
The role of surgery in patients with metastatic pulmonary sarcomatoid carcinoma (PSC)：a SEER‑based study
Authors: Hao Ligang , Zhang Junjie , Di yonghui 
Published: October 2023

Abstract

ObjectiveIn an effort to explore the prognostic role of surgery in patients with metastatic pulmonary sarcomatoid carcinoma, we performed a population-based study using the SEER data.MethodsPatients with metastatic PSC were identified from the Surveillance, Epidemiology, and End Results (SEER) database from 2000 to 2019. Hazard ratios (HR) and 95% confidence intervals (95% CI) were calculated by univariate and multivariate analysis using the Cox regression analysis, α = 0.05. Survival curves were plotted by using the Kaplan-Meier method and survival curves were compared between groups with the log-rank test.ResultsA total of 931 patients were finally enrolled and 88 patients received surgery to the primary tumor site. Surgery was independently associated with improved outcome in patients with metastatic PSC. Median overall survival was 8.0 months (95% confidence interval [CI], 7.0 to 15.0) with surgery versus 3.0 months (95% CI, 3.0 to 4.0) without surgery (hazard ratio for death, 0. 576; 95% CI, 0.447 to 0.743;P<0.0001). The overall survival benefit was consistent regardless of the brain metastasis, radiotherapy and chemotherapy. Patients also benefited more from resection of at least one lobe and mediastinal lymph node dissection (HR = 0.484, 95%CI 0.289–0.812, P = 0.0035; HR = 0.441, 95%CI 0.267–0.728, P = 0.00064).ConclusionsAccording to this study, surgery, especially lobectomy, bilobectomy or pneumonectomy with lymph node dissection, can prolong survival of patients with metastatic PSC. Treatment options for metastatic PSC should be based on multidisciplinary decision, and the possibility of surgery on primary tumor requires reasonable assessment.
Idiosyncratic Deals and Breakthrough Innovation: Based on the Paradox Theory Perspective
Authors: Fan Zili , Li Yitong , Wu Yige , Peng Ting , Peng Ziqian , Xue Jinli 
Published: October 2023

Abstract
Purpose: Scientific and technological independence depends on breakthrough innovation, and the breakthrough of innovation comes from the stimulation of talent vitality. Taking the idiosyncratic deals to meet the unique needs of talents as the starting point, this study constructs a two-stage moderating mediated model based on paradox theory, and discusses the impact of idiosyncratic deals on breakthrough innovation through exploratory-exploitative learning tension, as well as the moderating role of transactive memory systems and cognitive flexibility in this process.
Participants and Methods: In order to reduce the effects of common method biases and causal lag effect, this study was divided into three stages for data collection, with a time interval of a month. 332 employees from four enterprises in Shanghai and Wuhan were selected as samples, and the hypothesis test was carried out by linear regression, bootstrap and Johnson-Neyman method.
Results: The results showed that Idiosyncratic deals has a positive impact on breakthrough innovation through exploratory-exploitative learning tension; transactive memory systems in the first stage and cognitive flexibility in the second stage positively moderate the mediating effect of exploratory-exploitative learning tension between idiosyncratic deals and breakthrough innovation and. Under the combined effect of high transactive memory systems and high cognitive flexibility, idiosyncratic deals has the strongest indirect effect on breakthrough innovation through exploratory-exploitative learning tension.
Conclusion: The study results break through the previous research framework of social exchange theory, and use paradox theory to explore the influence mechanism of idiosyncratic deals, in order to promote the sustainable growth of breakthrough innovation through this non-standard work arrangement.
Progesterone decreases isoflurane requirement for both balance disturbance and loss of righting reflex in male mice
Authors: Shimizu Takeru , Nakamura Shoko , Inomata Shinichi 
Published: October 2023

Abstract
It has been known that progesterone has central effects, as measured by minimum alveolar concentration in various experimental settings. Previously we showed that progesterone reduces sevoflurane requirement for the loss of righting reflex (LORR) using male mice. However, the combination of progesterone and isoflurane has not been studied. Therefore, we tested the effect of progesterone on isoflurane requirement. Male C57BL/6 mice were treated with either progesterone (75 mg/kg) + olive oil or only olive oil. Animals were studied in closed cylinder supplied with oxygen and isoflurane that rotated 4 times per min. Balance disturbance and loss of the righting reflex were counted. The data were analyzed by using a multiple independent variable logistics regression model. Concentrations for the onset of balance disturbances; the effective dose 50% (ED50) and effective dose 95% (ED95) of isoflurane for control group and progesterone group were 0.37% and 0.45%, 0.34% and 0.41%, respectively. Concentrations for LORR; the ED50 and ED95 for control group and progesterone group were 0.55% and 0.62%, 0.53% and 0.60%, respectively. Subcutaneous injection of progesterone 75 mg/kg decreased isoflurane requirement significantly for both balance disturbance (p=0.0022) and LORR (p=0.0218). We conclude that progesterone decreased isoflurane concentration for both balance disturbance and LORR.
Effect of Temperature on Permeability of Mudstone Subjected to Triaxial Stresses and Its Application
Authors: Yu Jieqing , Dong Fuke , Zhou Yu , Wang Xingyan , Zhang Liang , Su Hailong 
Published: October 2023

Abstract

To prevent the leakage of pyrolyzed oil and gas as well as the release of pollutants from the top and floor strata, it is crucial to conduct a comprehensive study on the seepage characteristics of these strata under high-temperature triaxial stress conditions. This study will help ensure the containment and integrity of subsurface reservoirs and storage environments. Mudstone, serving as both the upper and lower strata, offers an effective barrier due to its inherently low permeability. In order to explore the change rule of mudstone sealing performance under high temperature triaxial stress, an air-heated low permeability rock mass air permeability measurement system is used to measure the ground stress buried 500m deep and the temperature variation characteristics of mudstone permeability on the roof and floor of Jimsar oil shale in Xinjiang under 100℃. It was found that the permeability of stressed mudstone decreased with the temperature rising up to 100℃. The predominant factor was thermal expansion of mudstone. The drop value depended on triaxial stresses that large stresses could induce small drop value. The average hydraulic radius of pore in the mudstone was also calculated, which also exhibited continuous reduction as heating up to 100℃ and the degree of reduction could reach 68%. The capacity, that prevent oil & gas and contaminant from moving cross strata as a barrier, would be strengthened when the mudstone strata from roof and floor experienced the temperature low than 100℃. The barrier performance of mudstone as a pollutant migration barrier layer to gas pollutant migration during in-situ heat injection mining of oil shale was further evaluated.
EMID2 is a novel biotherapeutic for aggressive cancers identified by in vivo screening
Authors: Cappelletto Ambra , Alfì Edoardo , Volf Nina , Bortolotti Francesca , Ciucci Giulio , Vodret Simone , Fantuz Marco , Perin Martina , Colliva Andrea , Rozzi Giacomo , Rossi Matilde , Ruozi Giulia , Zentilin Lorena , Vuerich Roman , Borin Daniele , Lapasin Romano , Piazza Silvano , Chiesa Mattia , Lorizio Daniela , Kumar Sandeep , Rustighi Alessandra , Jo Hanjoong , Piccolo Stefano , Carrer Alessandro , Giacca Mauro , Sal Giannino Del , ZACCHIGNA SERENA 
Published: October 2023

Abstract
Background. New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them are doomed to fail in clinical trials, largely because they are identified by cellular or in silico screens that cannot predict their in vivo effect. Methods. We screened an Adeno-Associated Vector secretome library (> 1000 clones) directly in vivo in a mouse model of cancer and validated the therapeutic effect of the first hit, EMID2, in both orthotopic and genetic models of lung and pancreatic cancer. Results. EMID2 overexpression inhibited both tumor growth and metastatic dissemination, consistent with prolonged survival of patients with high levels of EMID2 expression in the most aggressive human cancers. Mechanistically, EMID2 inhibited TGFβ maturation and activation of cancer-associated fibroblasts, resulting in more elastic ECM and reduced levels of YAP in the nuclei of cancer cells. Conclusions. This is the first in vivo screening, precisely designed to identify proteins able to interfere with cancer cell invasiveness. EMID2 was selected as the most potent protein, in line with the emerging relevance of the tumor extracellular matrix in controlling cancer cell invasiveness and dissemination, which kills most of cancer patients.
Identification of a Potential sialylation-related pattern for the Prediction of Prognosis and Immunotherapy Response in in Small Cell Lung Cancer
Authors: Yu Yao , Shang Yu , He Yaowu , Shi Si , Wang Qi , Ma Jing , Wang Menghan , Shi Wenchao , Chen Hong 
Published: October 2023

Abstract
Background: Our study aimed to establish a novel system for quantifying sialylation patterns and comprehensively analyze their relationship with immune cell infiltration (ICI) characterization, prognosis, and therapeutic sensitivity in small cell lung cancer (SCLC).
Methods: We conducted a thorough assessment of the sialylation patterns in 100 patients diagnosed with SCLC. Our primary focus was on analyzing the expression levels of 7 prognostic sialylation-related genes (SRGs). To evaluate and quantify these sialylation patterns, we devised a sialylation score (SS) using principal component analysis algorithms. Prognostic value and therapeutic sensitivities were then evaluated using multiple methods. The GSE176307 was used to verify the predictive ability of SS for immunotherapy.
Results: Our study identified two distinct clusters based on sialylation patterns. Sialylation cluster B exhibited a lower level of induced ICI therapy and immune-related signaling enrichment, which was associated with a poorer prognosis. Furthermore, there were significant differences in prognosis, response to targeted inhibitors, and immunotherapy between the high and low SS groups. Patients with high SS were characterized by decreased immune cell infiltration, chemokine and immune checkpoint expression and poorer response to immunotherapy, while the low SS group was more likely to benefit from immunotherapy.
Conclusion: This work showed that the evaluation of sialylation subtypes will help to gain insight into the heterogeneity of SCLC. The quantification of sialylation patterns played a non-negligible role in the prediction of ICI characterization, prognosis and individualized therapy strategies.
Advancing Intrusion Detection Efficiency: A ’Less is More’ Approach via Feature Selection
Authors: Sahin Onur , Uludag Suleyman 
Published: October 2023

Abstract
Cybersecurity relies heavily on effective intrusion detection, a task that may fall short when utilizing unprocessed data in machine learning models. In an endeavor to improve detection rates, our research embraced a ‘Less is More’ strategy. By employing Random Forest feature selection, the in- terpacket arrival time (IAT) surfaced as the key determinant, in a real-time dataset encompassing 33 attacks in an IoT environment consisting of 105 devices. Concentrating on this singular feature and reducing the data’s di- mensions (thus drastically minimizing training and prediction time), our best model yielded an F1 score of 90.46%, outperforming prior results by nearly 19%. Additionally, a trial using the most important 25 features yielded an F1 score of 84.26%. While this was not as successful, it may yield better results when experimenting with different datasets. We also measured training time and prediction time per entry for all models and stated that lower dimension- ality in data leads to drastically lower training and prediction times. Though the IAT-centered method showed considerable promise, its universal applica- bility may be limited. Our findings illuminate the substantial potential of this method in intrusion detection, emphasizing the crucial role that feature se- lection can play in enhancing accuracy, with effects that could be far-reaching across various real-world scenarios and scholarly pursuits. The limitation of this research lies in the potential inability to apply the one-feature approach universally. Moving forward, investigations may pivot towards assessing the ’Less is More’ strategy’s adaptability across diverse datasets, fine-tuning the approach to harmonize efficacy and applicability. This investigation not only underscores the potential of feature selection in intrusion detection but also manifests a breakthrough in efficiency, achieving a remarkable improvement over previous methods. By employing a focused approach, our research has catalyzed an advancement in the field. The substantial enhancement in detec- tion efficiency validates this approach, positioning it as a viable and effective solution for those seeking to strengthen and streamline intrusion detection systems.