bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brassica microgreens cabbage (Brassica oleracea), radish (Raphanus sativus) and rocket (Eruca vesicaria) (L.) Cav: application of red‐light emitting diodes lighting during postharvest storage and in vitro digestion on bioactive compounds and antioxidant activity
Authors: Ntsoane Manyasha L. L. , Manhivi Vimbainashe E. , Shoko Tinotenda , Seke Faith , Sultanbawa Yasmina , Sivakumar Dharini 
Published: January 2024

SummaryMicrogreens grow best under light‐emitting diodes (LED), an artificial light source. In this study, LED lights (red, blue and far‐red) were tested on brassica microgreens to see if they induced an increase in bioactive compounds (glucosinolates and phenolics). In vitro digestion also measured bioavailable bioactive compounds in the intestinal phase. LED lights (red, blue and far‐red) were applied for 6 h in storage at 5 °C and 85% RH for 5 days on cabbage (Brassica oleracea), radish (Raphanus sativus) and rocket (Eruca vesicaria (L.) Cav). Red light significantly enhanced ascorbic acid, total phenols, kaempferol and quercetin glycoside concentrations in all three Brassica microgreens and antioxidant activities. Exposure to red LED light increased 4‐methoxyglucobrassicin (cabbage), glucoraphenin (radish), glucoraphanin A and glucoerucin (rocket). Red light stress may have resulted in secondary metabolite production in immature plants. An in vitro digestion showed higher concentrations of phenolic compounds, glucosinolate components and antioxidants in the intestinal phase. Red LED light is recommended as a postharvest treatment for improving cabbage, rocket and radish bioactive compounds.
Exploring the potential applications of biopeptides from seaweeds, insects, and food wastes: mechanisms, utilisation, and industry implications
Authors: Le Thanh‐Do , Suttikhana Itthanan , Ashaolu Tolulope Joshua 
Published: January 2024

SummaryThe quest for a green and sustainable environment has created innovative ways of recycling and valorising agro‐based wastes, as well as using uncommon raw materials for food products. Likewise, food proteins and peptides are potentially generated from unique sources such as seaweeds, insects, hemp, rapeseed cake, olive meal cake, sesame meal, flaxseed cake, sunflower meal, spent grain, pomace, and pit for the development of functional foods and biopeptide‐based products. These biopeptides confer numerous positive health benefits against chronic diseases such as cancer, obesity, type 2 diabetes, hypertension, and neurodegeneration. In this review, diligent efforts were made to provide an updated status on the biopeptides produced from various unusual sources with certain salient considerations towards their production, biological activities, action mechanisms, and safety. We also discussed the possible drawbacks encountered during their applications in the nutraceutical and functional food industries.
Mental health and lifestyle behavior changes during COVID‐19 among families living in poverty: A descriptive phenomenological study
Authors: Zahry Nagwan R. , Ling Jiying , Robbins Lorraine B. 
Published: January 2024

AbstractProblemFamilies living in poverty may be particularly vulnerable to the deleterious effects of the coronavirus disease (COVID‐19). The current study focuses on caregivers of preschoolers aged 3–5 years old who lived in poverty to explore how COVID‐19 affected mental health and lifestyle behaviors and what coping strategies were helpful for dealing with challenges induced by COVID‐19.MethodsA qualitative study was conducted using individual semi‐structured interviews with 17 caregivers.FindingsBoth caregivers and preschoolers experienced high levels of stress, anxiety, and depression and reported unhealthy eating habits and excessive screen time. Physical activity increased in preschoolers, but declined among caregivers during COVID‐19. Caregivers’ coping strategies included taking short walks, seeking professional and social support, and praying; avoidant caregiver coping strategies including eating when stressed and increased screen time.ConclusionsFuture interventions are warranted to empower caregivers who live in poverty with the skills needed to maintain optimal physical and mental health, as well as assisting them to cope with challenges that may continue post‐COVID‐19.
Effectiveness of an evidenced‐based cognitive behavioral therapy intervention for adolescents in a school setting
Authors: Gray Sarah E. , Carter Michaela , Harper Amanda 
Published: January 2024

AbstractProblemThe adolescent years are a formative time when rapid hormonal and physical changes stimulate the developing mind. Exposure to poverty, abuse, violence, and lack of peer and social support causes an increase in vulnerability to the development of mental health problems. The COVID‐19 pandemic has also exacerbated symptoms of depression and anxiety. Regardless of the risk factors, anxiety and depression continue to be significant health problems, affecting thousands of adolescents yearly in the United States. The first‐line treatment recommendation for managing anxiety and depression symptoms is cognitive behavioral therapy (CBT). However, access to a provider for CBT treatment is not always an option for many reasons.MethodsThis pilot evidenced‐based practice project aims to increase access to skills acquired through CBT and improve adolescents' mental health by implementing a brief and evidenced‐based CBT program in a school setting. The CBT program for this project is the Creating Opportunities for Personal Empowerment (COPE) for Teens program consisting of seven 50−55‐min sessions. The COPE program was delivered to 22 students in a 7th‐grade health class. The Generalized Anxiety Disorder 7‐Item and Patient Health Questionnaire‐9 Modified for Adolescents measured anxiety and depression scores at baseline, post‐intervention, and 2‐month follow‐up.FindingsResults indicate clinically significant improvements in anxiety and depression scores and showed participant satisfaction.ConclusionsCOPE in the school setting offers a low‐risk solution to improving behaviors and emotional intelligence. COPE is a cost‐effective solution to the mental health provider shortage.
A systematic and comprehensive protocol for rapid orthodontic extrusion
Authors: Crljenica Mirjam Uravić , Perasso Roberto , Imelio Monica , Viganoni Chiara , Pozzan Lucia 
Published: January 2024

AbstractObjectiveThe aim of the present paper is to describe a systematic and detailed protocol for rapid orthodontic extrusion, and to summarize the main results of this protocol in terms of migration of dental, hard and soft tissues, for 10 cases.Clinical considerationsThe working design was to apply a protocol lasting 4 weeks of active phase with intense forces (greater than 300 g), perform weekly the circumferential supra‐crestal fiberotomy procedure associated with root‐planning, follow up with a passive stabilization phase of another 4 weeks and evaluate the results.ConclusionsWe believe that despite the partial migration of periodontal tissue, the defined protocol we used is, for simplicity of application, brevity of treatment and results, a viable alternative to the surgical technique of clinical crown lengthening in those cases where it is necessary to have more dental tissue exposed for restorative and prosthetic purposes.
Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft‐shell turtles
Authors: Chen Mengmeng , Xue Mingyang , Chen Jingtao , Xiao Zidong , Hu Xiaowei , Zhang Chunjie , Jiang Nan , Fan Yuding , Meng Yan , Zhou Yong 
Published: January 2024

AbstractAeromonas jandaei is a gram‐negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft‐shell turtle (Pelodiscus sinensis), then named strain JDP‐FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature‐sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP‐FX. Compared with healthy Chinese soft‐shell turtle, the serum levels of total protein (TP), albumin (ALB) and globulin (GLB) were significantly decreased in the diseased Chinese soft‐shell turtle, while, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased. Histopathological observations showed that multiple tissues, including intestinal mucosa, liver and kidney, were severely damaged in the diseased Chinese soft‐shell turtle. Moreover, the diseased Chinese soft‐shell turtle had significant cell degeneration, necrosis, sloughing and interstitial inflammatory cell infiltration. The pathogenicity of JDP‐FX was tested via artificial infection. The median lethal dosage (LD50) of the strain was 1.05 × 105 colony forming units (CFU/g) per weight of Chinese soft‐shell turtle. Drug susceptibility analysis revealed that JDP‐FX was susceptible to ceftazidime, minocycline, cefoperazone, ceftriaxone and piperacillin. In addition, JDP‐FX was resistant to doxycycline, florfenicol, sulfonamides, gentamicin, ampicillin and neomycin. Therefore, this study may provide guidance for further research into the diagnosis, prevention and treatment of JDP‐FX infection.
Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish
Authors: Li Zhicheng , Zhuang Jingyu , Chen Jiaming , Cao Jizhen , Han Qing , Luo Zhi , Wang Baotun , Wang Hebing , Li Anxing 
Published: January 2024

AbstractAmyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti‐parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular levels. The present work developed a new cell line, ALG, from the gill of yellowfin seabream (Acanthopagrus latus). The cell line was routinely cultured at 28°C in Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum (FBS). ALG cells were adherent and exhibited an epithelioid morphology; the cells were stably passed over 30 generations and successfully cryopreserved. The cell line derived from A. latus was identified based on partial sequence amplification and sequencing of cytochrome B (Cyt b). The ALG was seeded onto transwell inserts and found to be a platform for in vitro infection of A. ocellatum, with a 37.23 ± 5.75% infection rate. Furthermore, scanning electron microscopy (SEM) revealed that A. ocellatum parasitizes cell monolayers via rhizoids. A. ocellatum infection increased the expression of apoptosis and inflammation‐related genes, including caspase 3 (Casp 3), interleukin 1 (IL‐1), interleukin 10 (IL‐10), tumour necrosis factor‐alpha (TNF‐α), in vivo or in vitro. These results demonstrated that the in vitro gill cell monolayer successfully recapitulated in vivo A. latus host responses to A. ocellatum infection. The ALG cell line holds great promise as a valuable tool for investigating parasite–host interactions in vitro.
Knowledge, attitude, and practice of critical care nurses and their influence on acute skin failure patients in China: A multicenter cross‐sectional study
Authors: Fu Lisang , Chen Yiyuan , Lin Libin , Xu Zhenmei , Zhuang Yaoning 
Published: January 2024

AbstractAimAcute skin failure (ASF) can happen in critically ill patients. Therefore, adequate knowledge and skills regarding ASF are essential to manage this disorder. The aim of this work was to investigate the nurses' knowledge, attitude, and practice (KAP) regarding ASF patients and to analyze its influencing factors in the intensive care unit.MethodsA multicenter cross‐sectional study was performed in 10 tertiary hospitals in March 2022. Eligible nurses received a survey with a self‐designed questionnaire about their KAP regarding ASF, which included three dimensions and a maximum score of 120. A higher score corresponded to better KAP.ResultsA total of 255 nurses participated in this study. The mean KAP score was 75.6 ± 16.9. The mean scores of the knowledge, attitude, and practice dimensions were 26.2 ± 10.6, 38.2 ± 6.1, and 11.3 ± 4.3, respectively. The dimensions were ranked according to score (from low to high) as follows: knowledge (2.6 ± 1.1), practice (2.8 ± 1.1), and attitude (3.8 ± 0.6). Multivariate linear regression analysis showed that more training sessions and an advanced professional title were associated with higher KAP scores.ConclusionsThe overall KAP score on ASF was low in nurses. Nurses had a positive attitude towards ASF, but their knowledge and practice required significant improvement. Those who had received more training and held a more advanced professional title exhibited higher KAP scores regarding ASF. Therefore, nurse managers should establish a relevant knowledge training system, strengthen relevant training, and improve nursing practices for ASF in order to achieve a high level of KAP in nurses who treat ASF patients.
Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection
Authors: Setyawaty Dyah , Mariya Silmi , Prabandari Silvia A. , Boilessen Ditte Rahbaek , Holst Peter Johannes , Darusman Huda Shalahudin 
Published: January 2024

AbstractBackgroundCervical cancer is an abnormal growth of cervical tissue epithelial cells due to persistent human papilloma virus (HPV) infection. Cynomolgus monkeys (Macaca fascicularis) can be naturally and spontaneously infected with M. fascicularis Papillomavirus Type 3 (MfPV3), a virus that is phylogenetically closely related to human oncogenic HPV (HPV‐16 and HPV‐34), and therefore a potentially beneficial for modeling HPV disease. This study aims to evaluate the expression of the integrin alpha 6 (ITGα6) receptor in cynomolgus monkeys spontaneously infected with MfPV3, which this receptor also found in human infected with HPV.MethodsThe study was done on archived Formalin‐fixed Paraffin‐Embedded (FFPE) samples of uterine and cervix tissue of cynomolgus monkeys. Immunohistochemistry was also performed to quantify the expression levels of ITGα6.ResultsThe results showed 80% of the samples positive Cervical Intraepithelial Neoplasia (CIN) and increased expression of ITGα6 significantly in Positive‐MfPV3 group than negative‐MfPV3 group.ConclusionsThis indicated the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection type.
Predicting preterm birth within 2 weeks in asymptomatic women with a short cervix: Combined effects of cervicovaginal fluid cytokine levels and fetal fibronectin test
Authors: Ohori Yukiko , Imai Kenji , Tano Sho , Owaki Taro , Miki Rika , Nozaki Yuki , Ushida Takafumi , Kajiyama Hiroaki , Kotani Tomomi 
Published: January 2024

AbstractAimsTo predict preterm birth (PTB) accurately, we conducted a comprehensive cytokine assay using cervicovaginal fluid (CVF) and evaluated the additive effects of cytokine levels on the fetal fibronectin (fFN) test.MethodsA total of 645 CVF samples were collected from 256 asymptomatic pregnant women between 24 and 35 weeks gestation, exhibiting short cervix. After selection based on specific criteria, 17 cytokines in 105 CVF samples were simultaneously measured using multiplex assay. Multivariate logistic regression analysis was performed to evaluate the association between cytokine levels and impending PTB, which is defined as PTB within 2 weeks after CVF collection. Moreover, receiver operating characteristic (ROC) analysis was performed in women with positive fFN results, which was validated using another set of 65 CVF samples.ResultsIn positive fFN women, the CCL2 level was significantly higher in the impending PTB group than the other group (p < 0.01) and a predictor of impending PTB (adjusted odds ratio 1.020, 95% confidence interval [95% CI] 1.003–1.038, p = 0.020). The cutoff value of CCL2 was 64.8 pg/mL (are under the curve 0.726, p = 0.004, 95% CI 0.593–0.859, sensitivity 45.2%, specificity 91.7%). Additionally, the reliable classification performance of proposed ROC model could be validated. However, measuring cytokine levels could not help in predicting impending PTB in women with negative fFN or normal labor onset in healthy‐term women.ConclusionComprehensive analysis of CVF cytokines revealed that the CCL2 level significantly improves the prediction of impending PTB in asymptomatic fFN‐positive women with a short cervix, which may contribute to better clinical management.
Personality dynamics turn positive and negative mood into creativity
Authors: Bledow Ronald , Kühnel Jana , Kuhl Julius 
Published: January 2024

AbstractIntroductionResearch on the link between affect and creativity rests on the assumption that creativity unfolds as a stimulus‐driven response to affective states. We challenge this assumption and examine whether personality dynamics moderate the relationships between positive and negative mood with creativity.Theoretical ModelAccording to our model, personality dynamics that generate and maintain positive affect and downregulate negative affect energize creativity. Based on this model, we expect high creativity in response to negative mood if people engage in self‐motivation and achieve a reduction in negative mood. We further derive that individual differences in action versus state orientation moderate the within‐person relationship between mood and creativity.MethodWe conducted an experience‐sampling study and examined the relationship between mood and creativity in everyday work‐life. Two hundred and ten participants indicated their action‐state orientation and reported their mood three times a day over five consecutive workdays. At noon of each day, we assessed self‐motivation and in the evening the extent to which participants had generated novel and useful ideas during the day.ResultsWe observed high creativity when negative mood declined and self‐motivation was high. Action‐state orientation moderated the within‐person relationships of positive and negative mood with creativity.ConclusionPersonality dynamics determine whether positive and negative mood result in creativity.
ACVIM consensus statement on diagnosis and management of urinary incontinence in dogs
Authors: Kendall Allison , Byron Julie K. , Westropp Jodi L. , Coates Joan R. , Vaden Shelly , Adin Chris , Oetelaar Garrett , Bartges Joe W. , Foster Jonathan D. , Adams Larry G. , Olby Natasha , Berent Allyson 
Published: January 2024

AbstractUrinary incontinence (UI) is a disorder of micturition that can occur in dogs of any age, sex, and breed depending on the underlying cause and time of onset. Diagnosis and treatment for various causes of UI in dogs have been described by multiple comprehensive single author review articles, but large prospective clinical trials comparing treatment outcomes in veterinary medicine are lacking. The objectives of this consensus statement therefore are to provide guidelines on both recommended diagnostic testing and treatment for various causes of UI in dogs. Specifically, pathophysiology directly related to the canine urinary system will be reviewed and diagnostic and therapeutic challenges will be addressed. A panel of 12 experts in the field (8 small animal internists [L. Adams, J. Bartges, A. Berent, J. Byron, J. Foster, A. Kendall, S. Vaden, J. Westropp], 2 neurologists [J. Coates, N. Olby], 1 radiologist [G. Oetelaar], and 1 surgeon [C. Adin]) was formed to assess and summarize evidence in the peer‐reviewed literature and to complement it with consensus recommendations using the Delphi method. Some statements were not voted on by all panelists. This consensus statement aims to provide guidance for management of both male and female dogs with underlying storage or voiding disorders resulting in UI.
Taxonomic richness and its relationship to the functional diversity of polychaetes in tropical estuaries
Authors: Gomes Wilma Izabelly Ananias , Jovem‐Azevêdo Daniele , de Melo Dalescka Barbosa , Veríssimo Maria Eduarda Santana , Milesi Silvia Vendruscolo , dos Santos Paulo Jorge Parreira , Molozzi Joseline 
Published: January 2024

AbstractBiological diversity can be evaluated by the taxonomic and functional components. This study aimed to assess the relationship between taxonomic richness with the functional diversity components [functional richness (FRic), functional evenness (FEve) and functional divergence (FDiv)] and the functional trait dominance of polychaetes. In addition, we evaluated the environmental parameters that shape the functional structure in six tropical estuaries in north‐eastern Brazil. Three estuaries have a continuous environmental gradient (typical tropical), and the other three estuaries have no defined environmental gradient (semiarid tropical). We identified significant differences in the dominance of feeding strategy and habitat between estuaries, demonstrating that the functional space of the communities is not equally occupied. Also, the substrate particle size composition was correlated with the functional structure. FRic showed a positive relationship with taxonomic richness, regardless of the type of estuary. As taxonomic richness increases, the probability of different combinations of functional categories added to a community also increases. FEve and FDiv seem to be independent of taxonomic richness. Therefore, it is important to simultaneously evaluate diversity's taxonomic and functional components, considering the complementarity of the information provided by these diversity measures.
Associations of fish and meat intake with iron and anaemia in Malawian children
Authors: Werner E. Rochelle , Arnold Charles D. , Caswell Bess L. , Iannotti Lora L. , Maleta Kenneth M. , Stewart Christine P. 
Published: January 2024

AbstractAnimal flesh foods are rich in bioavailable iron but infrequently consumed by young children. We aimed to determine whether flesh food intake was associated with iron and anaemia status among 585 Malawian infants enroled in a 6‐month egg‐feeding trial. The percentage of days of small fish, large fish and meat consumption were assessed through weekly 7‐day animal‐source food screeners. Grams of intake were assessed through 24‐h recalls conducted at 6–9, 9–12 and 12–15 months of age. Plasma ferritin, soluble transferrin receptor (sTfR) and haemoglobin concentrations were measured at 6–9 and 12–15 months of age. Iron biomarkers were adjusted for inflammation during analysis. At enrolment, each flesh food category was consumed by <5% of children in the past 24 h. Over the next 6 months, small fish, large fish and meat were consumed on 25%, 8% and 6% of days, respectively, with mean usual intakes of <5 g/day. More frequent small fish consumption was associated with lower sTfR (geometric mean ratio [95% CI]: 0.98 mg/L [0.96, 1.00] per 10 percentage point difference) but not ferritin (1.03 µg/L [0.98, 1.07]) or haemoglobin (1.01 g/dL [1.00, 1.01]). Large fish consumption was associated with higher anaemia (prevalence ratio [95% CI]: 1.09 [1.01, 1.19]) and lower iron deficiency (0.96 [0.93, 1.00]) prevalence. Gram intakes of flesh food categories were not associated with any iron or anaemia indicators. Small fish were a primary contributor to flesh food intake in this cohort of Malawian children, although usual portions were small. Fish was associated with modest improvements to iron status, but meat was too infrequent to be associated with anaemia and iron deficiency.