bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Tridentate Hydrazone of Heterocyclic N‐oxide and Its Transition Metal Complexes: Evaluation of DNA/BSA Binding Interactions, In Vitro and In Silico Biological Properties
Authors: Deepa Samala , Nayakini Anitha , Mudavath Ravi , Lakshmi Padithem Jhansi , Sudeepa Kunche , Devi Ch. Sarala 
Published: January 2025

AbstractNovel hydrazone ligand 2‐(2‐(2‐hydroxy‐5‐methoxybenzylidene) hydrazinecarbonyl) pyridine 1‐oxide was synthesized and characterized by spectral analyses. The assessment of number of dissociable protons in ligand and evaluation of corresponding values of pKa and the stability constants for M(II)‐Ligand systems (M (II) = Cu, Co, Ni and Zn) in 30% DMF‐water medium were done by adopting pH‐metric method following Irving‐ Rossotti technique. To understand the chelation properties of hydrazone ligand, its contour maps of frontier molecular orbitals and their energies were computed using HyperChem 7.5 tools. The synthesized metal complexes of above hydrazone with Cu (II), Co (II), Ni (II) and Zn (II) were characterized with spectral and analytical techniques such as; FT‐IR, 1H‐NMR, LC‐MS, TGA, UV‐Vis, FE‐SEM‐EDX and ESR. The tridentate and dibasic nature of title ligand is ascertained from pH‐metric studies as well as through the analyses of analytical and spectral data of complexes. The CT‐DNA binding studies conducted adopting absorption and fluorescence titrations with metal complexes and ligand inferred their binding affinity through the mode of intercalation. The evaluated antioxidant and cytotoxicity properties revealed higher efficacy of copper complex. Furthermore, investigations employing molecular docking studies revealed non‐covalent bonding interactions of synthesized compounds with chosen protein target CDK2.
Catalyst Free Chemoselective Carbene Insertion Into COO─H Bond Over ─OH of Salicylic Acid
Authors: Jelakam Gruhapriya , Dey Swaraj , Wotsa Vikiho , Sivasankar Chinnappan 
Published: January 2025

AbstractThe α‐ salicylate esters are found in natural products and play a vital role in biological processes. Understanding their structures and functions can provide insights into biochemical pathways and potential therapeutic targets. Herein, we have developed a strategy without the use of metal catalysts or additives for the chemoselective carbene insertion into COO─H bond over ─OH of salicylic acid. Salicylic acids were reacted with diazo compounds to yield α‐salicylate esters at 150 °C. Having realized the importance of α‐salicylate esters, we have generalized our strategy to various diazo compounds as well as salicylic and thiosalicylic acids. All the reactions produced expected products in good to excellent yields. It has been observed that salicylic acid reacted with diazo compounds in a chemoselective manner while thiosalicylic acid produced a mixture of products. The plausible mechanism of the reaction has been supported by DFT calculations and the origin of the chemoselectivity has been realized with the help of local Fukui functions. All the synthesized α‐ salicylate esters were isolated and characterized by sophisticated analytical and spectroscopic techniques including single crystal X‐ray analysis.
Conditionally Activated ROS Generation by MMP2/9‐Specific C60‐Based Fluorescence Probes
Authors: Ma Yue , Persi Lorenzo , Yan Shen , Lee Sung Sik , Baumgartner Martin , Yamakoshi Yoko 
Published: January 2025

AbstractA C60‐coumarin conjugate, covalently connected via a matrix metalloproteinase (MMP2/9)‐cleavable peptide linker (Pro‐Leu‐Gly‐Val‐Arg‐Gly), is developed as a probe for both imaging and photodynamic treatment of MMP2/9‐expressing malignant cells. In the synthesized probe, the coumarin fluorescence is completely suppressed intramolecularly by the C60 moiety, while an intensive fluorescence increase is observed in the presence of MMP2/9 dependent on cleavage of the peptide linker. The specificity of the probe to detect MMP2 is confirmed by control experiments resulting in no emission 1) with a control probe bearing a shuffled peptide (Val‐Arg‐Leu‐Gly‐Pro‐Gly) or 2) in the presence of an MMP2 inhibitor (1,10‐phenantheoline). The probe is added to three types of tumor cell lines with different MMP2/9‐expression levels to perform in vitro cellular imaging. MMP2/9 is successfully detected by the probe in DAOY cells with the highest expression level of MMP2/9. Upon cleavage of the probe by MMP2/9, photoinduced 1O2 generation is much enhanced, resulting in the MMP‐dependent induction of higher photocytotoxicity of the probe in DAOY compared to HCT116 with much lower expression. The results suggest that the probe can serve as (I) a fluorescent reporter for MMP2/9 and (II) a photosensitizer for selective photodynamic treatment of malignant cells with MMP2/9 overexpression.
Light Assisted Covalent Immobilization of Proteins for Biomedical Applications
Authors: Pinho Vanessa , Neves‐Petersen Maria Teresa , Machado Raúl , Castro Gomes Andreia 
Published: January 2025

AbstractThe covalent immobilization of proteins attracts considerable interest in the biomedical field due to its potential applications in biosensors, recombinant protein purification, and the development of personalized therapeutic carriers. In response to the demand for more cost‐effective, time‐efficient, and simpler protocols, photo‐immobilization emerges as a technique that circumvents the limitations of conventional methods. This approach offers enhanced precision at the nanoscale level and facilitates device reusability, thereby aligning with current sustainability concerns. Photo‐immobilization is versatile, as it can be applied to both 2D and 3D substrates. While some methods involve complex protocols using genetically engineered photosensitive linkers, more straightforward techniques rely on amino acid bonds, such as disulfide bonds, for covalent protein bonding. Photo‐immobilization can be achieved with both ultraviolet (UV) and visible light. This systematic review examines literature from Scopus, PubMed, and Web of Science, offering insights into relevant studies and considerations for covalent protein immobilization, and presents photochemical approaches applicable to major protein types.
Double‐Layer Microneedle Patch Loaded with HA‐PBA‐QCT for Management of Paclitaxel‐Induced Peripheral Neuropathic Pain
Authors: Kong Yunfan , Pan Tianshu , Liu Bo , Kuss Mitchell , Krishnan Mena A. , Alimi Olawale A. , Shi Wen , Duan Bin 
Published: January 2025

AbstractChemotherapy‐induced neuropathic pain (CINP) is a common adverse effect of antineoplastic drugs, often leading to dose reduction, treatment delays, or cessation of chemotherapy. Chemotherapy agents, like paclitaxel (PTX), damage the somatosensory nervous system by inducing neuroinflammation and oxidative stress, resulting in the sensitization of sensory neurons. Quercetin (QCT), known for its anti‐inflammatory, antioxidant, and neuroprotective properties, is investigated for various neurological disorders. This work creates phenylboronic acid‐modified hyaluronic acid (HA‐PBA) gels with incorporated QCT and fabricates a double‐layer microneedle (MN) patch using an HA‐PBA‐QCT complex in the needles and HA/polyvinyl alcohol (PVA) as the substrate. The crosslinking between PVA and HA‐PBA‐QCT enables a controlled, sustained release of QCT upon application. This work applies these QCT‐loaded microneedle (QMN) patches to the instep skin of PTX‐treated mice, which exhibits mechanical allodynia and cold hyperalgesia. Biweekly applications of the QMN patches significantly reduce pain responses. This analgesic effect is associated with the modulation of satellite glial cell activity, decreased macrophage infiltration, and reduced TNF‐α and IL‐6 levels in dorsal root ganglia (DRGs). Additionally, the treatment improves cellular antioxidant capacity, indicated by upregulated Nrf2 and catalase in DRGs. Overall, these findings suggest that double‐layer QMN patches offer long‐term anti‐inflammatory and antioxidant benefits, potentially alleviating CINP in patients.
Experimental research on seismic performance of prefabricated beam–column joints with steel skeletons
Authors: Zhu Zhangfeng , Meng Hao , Zheng Yongfeng 
Published: January 2025

AbstractTo address the challenges of reinforcement congestion at prefabricated reinforced concrete beam–column joints and the high costs associated with the construction of composite beam–column joints, a prefabricated beam–column joint with a steel skeleton for prefabricated reinforced concrete columns and composite beams or steel beams is proposed to achieve construction convenience and cost savings. Three prefabricated specimens with different steel skeletons and one cast‐in‐place comparison specimen were fabricated and subjected to low‐cycle repeated horizontal loads. The experimental results indicated that, compared with the cast‐in‐place specimen, the three prefabricated specimens exhibited ductile behavior with the formation of a flexural plastic hinge and possessed comparable bearing capacity and stiffness, along with improved displacement ductility and energy dissipation capacity. Notably, the prefabricated specimen with a spiral stirrup steel skeleton exhibited the best overall performance. Furthermore, the results of a finite element parametric analysis based on the spiral stirrup steel skeleton specimen indicated that various parameters, such as the height of the steel plate frame and vertical stiffening ribs to the exterior extended steel, could improve the stress state of the steel skeleton without significantly impacting joint performance. Therefore, further optimization of the steel skeleton remains feasible to reduce steel usage and facilitate construction.
The role and mechanism of hepatocyte nuclear factor 1β in the occurrence and development of different human tumors: A pan‐cancer analysis
Authors: Li Liang , Li Haikun , Zhang Ke , Zhao Chunchun , Wang Fei , Sun Jian , Wang Jianqing 
Published: January 2025

AbstractCarcinomatosis is one of the leading threats to human public fitness. HNF1B is a critical transcription element in vertebrate proliferation and oncogenesis, which has been shown to play roles in reactive oxygen species (ROS) metabolism. Our previous results have identified HNF1B as a tumor suppressor that could inhibit the malignant progression of prostate cancer. Yet there is no pan‐carcinomatosis analysis of HNF1B, which could help us better understand common and unique underlying mechanisms in mankind knubs to enhance novel and competent treatment. Here, in our research, we evaluated the utterance pattern and explored the function of HNF1B in 33 knub categories using the data from the Cancer Genome Atlas Program (TCGA), Gene Expression Omnibus (GEO), and CLNICAL PROTEOMICTUMOR ANALYSIS CONSORTIUM (CPTAC) dataset. We found different HNF1B roles in various cancer types. HNF1B was upregulated in CHOL, STAD, KIRP, and THCA, and was downregulated in GBM, KICH, COAD, KIRC, LUSC, SARC, PAAD, and TGCT. Prognostic analyses indicated that higher HNF1B displayed better illness outcomes in BLCA, READ, and PRAD, while poorer outcomes in LUSC and THYM. HNF1B mutation was most frequent in endometrial cancer but was not associated with disease prognosis. It was discovered that HNF1B utterance relevant to endothelial cell penetration status in BLCA, ESCA, LUAD, LUSC, and TGCT, and carcinomatosis‐associated fibroblast infiltration was observed in ESCA, KIRC, LIHC, and TGCT. Moreover, functional enrichment analysis disclosed that metabolism‐related functions were implicated in the function of HNF1B. Taken together, our pan‐ carcinomatosis analysis showed the complicated roles of HNF1B in a variety of carcinomatoses, being able to improve the extensive comprehension of HNF1B's role in tumorigenesis.
Combining genome‐wide association and genomic prediction to unravel the genetic architecture of carotenoid accumulation in carrot
Authors: Rolling William R. , Ellison Shelby , Coe Kevin , Iorizzo Massimo , Dawson Julie , Senalik Douglas , Simon Philipp W. 
Published: January 2025

AbstractCarrots (Daucus carota L.) are a rich source of provitamin A, namely, α‐ and β‐carotene. Breeding programs prioritize increasing β‐carotene content for improved color and nutrition. Understanding the genetic basis of carotenoid accumulation is crucial for implementing genomic‐assisted selection to develop high‐carotenoid lines. While previous studies identified loci (Y2, Y, Or, and REC) associated with carrot color and carotenoid content, this study employed genome‐wide association (GWA) in a diverse panel of 738 carrot accessions. We discovered a novel locus with a candidate gene encoding phytoene synthase, a key enzyme in carotenoid biosynthesis. The Y2, Y, Or, and REC loci are mostly fixed in orange varieties, yet considerable variation in carotenoid concentration persists. This suggests a multigenic trait influenced by the environment. GWA of carotenoid concentration identified a quantitative trait locus for total carotenoids and α‐carotene. We explored the accuracy of genomic prediction (GP) models to predict carotenoid concentration. We determined the optimal number of plants and plots required for accurate carotenoid phenotyping, finding ≥5 plants per plot and three plots per site as the minimum effective sample per accession. GP models achieved accuracies ranging from 0.06 to 0.40 depending on the carotenoid measured and environment the carrots were assayed. Additional studies in breeding programs will clarify the potential of genomic‐assisted selection for high‐carotenoid carrots.
Green Human Resource Management (GHRM) and Corporate Social Responsibility (CSR) in Reducing Carbon Emissions for Sustainable Practices
Authors: Faeni Dewi Puspaningtyas , Oktaviani Retno Fuji , Riyadh Hosam Alden , Faeni Ratih Puspitaningtyas , Beshr Baligh Ali Hasan 
Published: January 2025

ABSTRACTThis study aims to explore the positive influence of green human resource management (GHRM) and corporate social responsibility (CSR) implementations on carbon emission reduction as an environmental control strategy in the transportation industry. This study is descriptive in nature and employs a quantitative approach. We derived the study's data from a literature analysis and collected primary data from 1080 CSR employees in the transportation industry using questionnaires. The results suggest that the application of the GHRM concept in the transportation sector contributes to a paradigm shift among employees toward ecological sustainability. To emphasize the environmental conservation and management approach, CSR initiatives include planting trees and mangroves, implementing 3R, water villages, community empowerment, renewable energy, and other initiatives. To reduce plastic use and improve waste segregation, COVID‐19 compelled all enterprises to implement a paperless policy and embrace the green transportation concept, thereby mitigating environmental impact. Maintaining a green culture at work is one way to recognize and thank staff members who consistently contribute to solving environmental problems, particularly those related to reducing carbon emissions. Moreover, the novelty of this study is that a holistic approach—combining green HRM, CSR, and operational changes—can foster a sustainable workforce and achieve environmental goals in the transportation sector. This research provides valuable insights for policymakers, managers, and academics interested in driving ecological sustainability through strategic human resource management and CSR initiatives.
Public Communication of Climate and Justice: A Scoping Review
Authors: Tschötschel Robin S. , Diamond Emily , Howley Shannon E. , McNally Brenda , Morris Hanna E. , Perry Kelly E. , Wilhelmsen Marthe Elden 
Published: January 2025

ABSTRACTThe intersection of public communication, climate change, and justice constitutes a nascent but growing interdisciplinary field of vital importance as climate change, driven largely by consumption patterns in high‐income counties, disproportionately affects communities with limited adaptive capacity, raising profound justice concerns. This scoping review delves into the emerging domain of public communication regarding climate and justice and seeks to provide a comprehensive overview that may help guide future research. It maps the landscape of existing peer‐reviewed journal scholarship, identifying trends and gaps across disciplines such as communication, energy politics, and urban planning. Following a birds‐eye quantitative analysis of English peer‐reviewed journal articles in the field (N = 250 studies), six thematic areas are scrutinized in‐depth: (1) activism and protest, (2) journalism and news media, (3) international negotiations, national policy, and local engagement, (4) art and cultural production, (5) climate obstruction and delay, and (6) communication effects on attitudes and behaviors. The review reveals, inter alia, a predominance of research originating from, and case studies focused on high‐income countries, a strong reliance on qualitative methods, and a tendency to conceive of justice in terms of distributive rather than procedural or representational questions. In the authors' view, the review indicates a need for comparative research, quantitative studies, and a broader inclusion of perspectives from regions disproportionately affected by climate change—particularly from low‐ and middle‐income countries. The authors call for a concerted effort to bridge the gap between activism and communication by emphasizing the critical role of justice‐oriented communication in fostering a fair and rapid transition to a sustainable future.
Microbiological findings in a cohort of patients with coronavirus disease 2019 and venovenous extracorporeal membrane oxygenation
Authors: Glück Christian , Widmeier Eugen , Maier Sven , Staudacher Dawid L. , Wengenmayer Tobias , Supady Alexander 
Published: January 2025

Abstract

Background
Venovenous extracorporeal membrane oxygenation (VV ECMO) is an established support option for patients with very severe respiratory failure and played an important role during the coronavirus disease 2019 (COVID-19) pandemic. Bacteria and fungi can lead to severe infectious complications in critically ill patients. The aim of this study was to describe the microbiological spectrum of bacteria and fungi detected in patients with COVID-19-associated respiratory failure supported with VV ECMO in our center.

Methods
This retrospective single-center analysis included all patients with COVID-19-associated respiratory failure supported with VV ECMO in our center between March 2020 and May 2022. All findings from microbiological samples, taken as part of clinical routine assessment from initiation of VV ECMO until day 30 were included. Samples were described by site and time of detection and microbiological characteristics.

Results
From March 2020 through May 2022, 88 patients with COVID-19-associated respiratory failure received VV ECMO support at our center. In 83/88 patients (94.3%), one or more pathogens were found in microbiological samples. Most pathogens were isolated from samples from the respiratory tract (88.6%). Earliest detection occurred in samples from the respiratory tract with a median time of 5 days to first detection. The most frequently detected pathogens were Staphylococcus spp., Candida spp., Klebsiella spp., Escherichia coli and Enterococcus spp.

Conclusion
In this cohort of severely ill COVID-19 patients receiving VV ECMO support, pathogens were frequently detected.
Whole-body PET imaging of simian immunodeficiency virus using gp120-targeting probes fails to reveal regions of specific uptake in rhesus macaques
Authors: Srinivasula Sharat , Kim Insook , Jang Hyukjin , Degrange Paula , Brown Heather , Dalton Viviana , Badralmaa Yunden , Natarajan Ven , Long Brad , Carrasquillo Jorge A. , Di Mascio Michele 
Published: January 2025

Abstract

Purpose
Following the initial reports demonstrating the feasibility of immunoPET imaging of simian immunodeficiency virus (SIV) using gp120-targeting monoclonal antibodies in non-human primates, replication efforts of the imaging system in human immunodeficiency virus (HIV)-infected individuals have yielded conflicting results. Herein, we used two anti-gp120 antibodies, 7D3 and ITS103.01LS-F(ab’)2, to interrogate the reproducibility of gp120-targeting probes for immunoPET imaging of SIV in rhesus macaques.

Methods
The binding affinity estimates of 89Zr radiolabeled 7D3 and ITS103.01LS-F(ab’)2 to SIV gp120, and the in-vitro and ex-vivo binding specificities of [89Zr]Zr-7D3 and [89Zr]Zr-ITS103.01LS-F(ab’)2 to SIV Env expressing cells, primary cells, and tissue sections from uninfected and SIV-infected macaques were obtained through competition assays. The biodistributions of [89Zr]Zr-7D3 and [89Zr]Zr-ITS103.01LS-F(ab’)2 were performed with static PET scans up to 6 days post-injection in 20 rhesus macaques and the standardized uptake values in various tissues were compared between SIV-infected and uninfected controls.

Results
Despite the demonstrated nanomolar affinity of [89Zr]Zr-7D3 and [89Zr]Zr-ITS103.01LS-F(ab’)2 to SIV gp120, and strong binding specificity to SIV gp120 cell lines, we observed no discernible differences in their binding in primary cells, tissue sections of secondary lymphoid organs, in-vivo probe uptake between SIV-infected and uninfected macaques, or ex-vivo validation necropsies. While the probes remained stable in-vivo, only [89Zr]Zr-ITS103.01LS-F(ab’)2 in chronic plasma retained its binding specificity to SIV gp120, with [89Zr]Zr-7D3 experiencing a > 97% reduction in binding to gp120 due to competition from endogenous antibodies at the 7D3 binding site.

Conclusion
The overall absence of specific uptake suggests inadequate binding potential (ligand affinity x target molarity) for these probes to effectively image SIV or HIV in-vivo, warranting further investigation into the lack of reproducibility observed with earlier non-human primate SIV imaging and conflicting human studies.