bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of late disorders as possible long-COVID and/or vaccination consequences
Authors: Larijani Mona Sadat , Bavand Anahita , Banifazl Mohammad , Ashrafian Fatemeh , Moradi Ladan , Ramezani Amitis 
Published: October 2023

Abstract
Background:Following SARS-CoV-2 outbreak, another crisis has been established by progress or persistence of COVID-19 symptoms, as post or long COVID, which is critical to be recognized according to increasing number of late symptoms reports. In this study, we investigated long-term disorders in vaccinated individuals who developed late adverse events/side effects post-vaccination and/or COVID-19 episode owing to the challenging differentiation between them.
Methods: The vaccinated individuals of four vaccine regimens including inactivated virus-based/prime-boost group, inactivated virus-based prime/subunit protein-based boost, adenovirus-based prime/boost and adenovirus-based prime/subunit protein-based boost were followed from the first dose of vaccination up to 6-month post the booster shot. All the vaccine adverse events were documented through in-depth interview using a researcher-made questionnaire as well as the COVID-19 history and demographics. The medical history of all the cases were also collected and the late disorders post-vaccination were investigated by Adverse Events Committee of Pasteur Institute of Iran.
Results: Totally 329 individuals who got 3 doses of vaccination were followed 6 months after the booster shots among whom 41 (12.4%) of investigated cases (including 31 females) with the mean age of 40.9±10.48 years had a type of disorder following vaccination. The late adverse events/side effects were captured in various forms among which gynecological and osteoarticular involvements were the most common ones. After evaluation of the medical background, age, vaccination and the date of disorder initiation, 39% of the documented complains have been found to be linked with long-COVID-19 as a trigger. Interestingly, the average time of symptoms persistence was 155±10.4 days and mostly continued up to the end of the study.
Conclusion: In this era in which the vast majority of the global population have developed COVID-19 and/or got vaccinated against it, identification of the late disorders as the vaccine side effect or long-COVID manifestation is difficult. Therefore, some complications though late ones, might be a possible consequence of any SARS-CoV-2 infection or vaccination. This study takes the advantage of long-term follow-up which presents various forms of late events in each episode of COVID-19 infection and vaccination. The rate of late-disorders in the present study also highlights the importance of long-term follow-up studies among population worldwide.
Data Security and Privacy Research Trends: LDA Topic Modeling
Authors: Zhao Bin , Wang Han , Zhou Jie 
Published: October 2023

Abstract

With the rapid advancement of big data technologies, the need for robust data security and privacy measures has intensified. Big data technologies have revolutionized the collection and analysis of a vast volume of research literature, offering unparalleled avenues for scholarly inquiry. Identifying prevalent research topics and discerning developmental trends is paramount, especially when grounded in an expansive literature base. This study examined abstracts and author keywords from 4,311 pertinent articles published between 1980 and 2023, sourced from the Web of Science core collection. The content of abstracts and author keywords underwent LDA theme modeling analysis. Consequently, five predominant research topics emerged: security and privacy measures for mobile applications, encryption protocols tailored for image security, privacy considerations in healthcare, intricate access control combined with security in cloud computing through attribute encryption, and ensuring security and information integrity for big data within the Internet of Things framework. The LDA model proficiently pinpoints these salient topics, assisting researchers in comprehending the current state of the domain and guiding potential future research trajectories.
Association of immune deficiency with prognosis and corticosteroids treatment benefits among patients with ARDS
Authors: Zhou Yanan , Hou Dongni , Chen Cuicui , Yang Yanping , Wang Ying , Song Yuanlin 
Published: October 2023

Abstract
Objective
To investigate the prognostic significance of immune deficiency in ARDS and its response to different doses of corticosteroids.
Methods
The study enrolled 657 patients and they were divided into discovery dataset (n = 357) and validation dataset (n = 300) according to the time of admission, and the relationship between immune deficiency and prognosis and response to corticosteroid therapy in patients with different severity were also analyzed.
Results
Immune deficiency was an independent prognostic factor affecting overall survival. Analysis showed that patients with mild to moderate ARDS (Discovery dataset: HR 1.719; 95% CI 1.229–2.406; Validation dataset: HR 1.874; 95% CI 1.238–2.837) or severe ARDS (Discovery dataset: HR 1.874; 95% CI 1.007–3.488; Validation dataset: HR 1.698; 95% CI 1.042–2.768) with immune deficiency had lower overall survival. And mild to moderate ARDS with immune deficiency benefit better from low dose corticosteroids (HR 0.409; 95% CI 0.249–0.671), severe ARDS with immune deficiency benefit better from both low (HR 0.299; 95% CI 0.136–0.654) and high dose corticosteroid treatment (HR 0.458; 95% CI 0.214–0.981).
Conclusions
Immune deficiency was considered to be an independent risk factor in ARDS patients, and its inclusion in the disease severity grading system based on PaO2/FiO2 was conducive to promoting personalized medication for ARDS patients.
CTX-M-127 with I176F mutations found in bacteria isolates from Bangladeshi circulating banknotes
Authors: Ali Md. Zannat , Sriniva Sankaranarayanan , Akter Selina 
Published: October 2023

Abstract
Extended-spectrum beta-lactamase (ESBL)-producing organisms are widely recognized as clinically relevant causes of difficult-to-treat infections. CTX-M has formed a rapidly growing family distributed worldwide among a wide range of clinical bacteria, particularly members of Enterobacteriaceae. Circulating banknotes, exchanged daily among people, pose a potential vehicle for transmitting multidrug resistance. In the present study, we screened for ESBL-carrying bacteria and reported CTX-M mutations in the banknotes of Bangladesh. We sequenced the genes and performed homology modeling using the Swiss model with CTX-M-15 (4HBT) as a template. Then, we performed molecular docking of mecillinam with the template and the generated model using Autodock 4.2. After docking, we visually inspected the complexes built using Autodock tools for polar contacts and pi-pi interactions in PyMOL 2.5.4. Our partially sequenced blaCTX−M was related to blaCTX−M−10 and blaCTX−M−15. We observed multiple single-nucleotide substitution mutations, i.e., G613T (silent mutation), A626T (I176F), and A503G (N135D). Homology modeling showed high similarity when the model was superimposed over the template. The orientation of Asn (135) in the template and Asp (135) in the model does not show a significant difference. Likewise, Ile (176) in the template and Phe (176) in the model offer the same orientation. Our generated model could bind to Lys237, Ser240, and Asp135 residues with the lowest binding energy on docking. Our predicted binding of the mecillinam to the mutated D-135 residue in the model indicates contributions and supports previous report proposing CTX-M-15 to CTX-M-127 mutational conversion on mecillinum resistance phenotype.
TRIM67 promotes the function of non-small cell lung cancer by positively regulating the Notch pathway through DLK1 ubiquitination
Authors: Jiang Jun , Dong Xinjie , Liu Jixuan , Liu Ting , Chen Xiaoshuai , Bian Xiaolei , Li Ming , Liu Yafang 
Published: October 2023

Abstract
Background:
Tripartite motif-containing 67 (TRIM67) is an E3 ubiquitin ligase that belongs to the TRIM protein family. We previously reported a relationship between TRIM67 and tumorigenesis. TRIM67 immunostaining results correlated with clinicopathological features. TRIM67 expression was associated with tumor size, lymph node metastasis, p-TNM stage, cancer cell differentiation, and poor prognosis. TRIM67 promoted cell proliferation, migration, and invasion by positively regulating the Notch pathway. Delta like non-canonical Notch ligand 1 (DLK1) is an atypical ligand that inhibits the receptor activity of Notch1, thereby inhibiting the activation of the Notch pathway.
Results:
In the present study, we demonstrated the mechanism by which TRIM67 modulates the Notch pathway. We found that TRIM67 induced the Notch pathway by ubiquitinating DLK1 through its RING domain, thereby affecting the function of non-small cell lung cancer (NSCLC) cells.
Conclusions:
These results suggested that TRIM67 plays a role in promoting the development of NSCLC.
Elevated serum neuropeptide Y levels are associated with carotid plaque formation in Chinese adults: A cross-sectional study
Authors: Wang Wan-da , Lin Hui-li , Zheng Yan-li , Wang Sheng-nan , Wang Yao-guo 
Published: October 2023

Abstract
Background
Carotid plaque (CP) formation is an important consequence of atherosclerosis and leads to significant complications. Levels of neuropeptide Y (NPY), which is a sympathetic neurotransmitter, are elevated in cardiovascular diseases. It also has important roles in inflammatory conditions. This study aimed to explore the relationship between serum NPY and CP and to study further the influence of NPY and inflammatory factors on CP.
Methods
This cross-sectional study was conducted among 300 adults who underwent a health examination at the Second Affiliated Hospital of Fujian Medical University in Fujian Province, of whom 177 were finally enrolled. The participants were divided into the CP (n = 120) and non-CP (NCP) or control (n = 57) groups according to the results of carotid artery color Doppler ultrasound. The CP group was further classified into stable plaque (SP, n = 80) and vulnerable plaque (VP, n = 40) groups based on plaque characteristics. Serum NPY and pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) levels were examined. Univariate and correlation analyses were used to evaluate the correlation between serum NPY levels, pro-inflammatory cytokines, and the CP phenotype.
Results
The serum NPY and TNF-α levels of patients in the CP group were significantly higher than those in individuals from the NCP group [ (177.30 ± 43.29) pg.mL-1 vs. (121.53 ± 40.16)pg.mL-1, P < 0.001; (41.94 ± 14.19) pg.mL-1 vs.(33.54 ± 13.37)pg.mL-1, P = 0.003]. The serum NPY levels of the patients in the VP group were significantly higher than those in patients from the SP group [(191.67 ± 39.87)ng.L-1 vs.(170.12 ± 43.37)ng.L-1, P = 0.01, P < 0.05]. Serum TNF-α and NPY levels were positively correlated among patients from the CP group (r = 0.184, P = 0.044). The binary logistic regression analysis showed that serum NPY and TNF-α were independent influencing factors of CP [(OR = 1.029, P < 0.001);(OR = 1.030, P = 0.023)] .
Conclusion
Together, elevated serum NPY levels seem to be associated with the occurrence of coronary atherosclerosis in Chinese adults.
Intraventricular metastases from small cell lung cancer: case report and literature review
Authors: Yang Chunjiao , Liao Meiyan , Xu Yu , Gan Tian , Liu Yumin 
Published: October 2023

Abstract
Background
Metastases are uncommon in intraventricular tumors. Intraventricular metastases from lung cancer are extremely rare and present challenges in terms of diagnosis and management.
Case Presentation:
We present the case of a 52-year-old man who presented with multiple lesions on CT and MRI. A lung CT scan, needle biopsy, and pathological examination confirmed the diagnosis of small cell lung cancer. The patient received chemotherapy with anti-PD-L1 blockade, along with etoposide and platinum (EP). As a result, the size of the intraventricular tumor was significantly reduced.
Conclusions
We summarized nine cases of intraventricular metastasis of lung cancer, including our case. Patients are often challenging to diagnose initially, especially in cases of undiagnosed lung cancer, and their prognosis is often unfavorable. On CT or MRI, intraventricular metastases of lung cancer appear as multiple lesions that are distributed along the ventricular wall. Intraventricular metastases should be considered when multiple neoplastic lesions are found within the ventricles. Conducting a search for primary tumor lesions or performing an intraventricular tumor biopsy can help confirm the diagnosis.
Zinc Nanoparticles for Contributing Salinity Stress Resilience in Wheat (Triticum aestivum L.)
Authors: SHUKLA GYANIKA , Singh Amardeep , Tyagi Anushka , Gaurav Shailendra Singh 
Published: October 2023

Abstract
Wheat is the most widely grown food crop in the world. The world is seeing much of its wheat yield decline due to salt stress. This study aimed to evaluate the effectiveness of zinc nanoparticles in improving resilience to salt stress in wheat (Triticum aestivum L.). ZnNP was biosynthesized using Crassula multicava leaf extract as reducing agent and capping agent. The synthesized nanoparticles were characterized to evaluate structural and biochemical aspects. The ZnNPs were found to be irregular in shape at 100nm magnification by TEM analysis. The average size of the biosynthesized ZnNPs was 64.6 nm as evidenced by DLS analysis. The experiment was laid out with a randomized complete block designed and arranged with eight replications in the pots with 5 rows having one row of control and the other 4 rows of different salinity levels such as 100mM, 75mM, 50mM, and 25mM. The crop was subjected to a total 3 treatments; The first NaCl stress was given on emergence of the Tillering stage (30 DAS) and after 2 days of salinity stress period the wheat plant pots were treated with 2mM zinc nanoparticles, second at the Booting stage (58 DAS) and third at Flowering stage. Plants that were not stressed with NaCl and not treated with NPs were considered as controls. Plant growth parameters were recorded. The results obtained showed that the application of ZnNP had a positive effect on all morphological and yield characteristics of wheat plants. The best results were recorded in potted plants subjected to 25 mM NaCl stress and treated with ZnNPs and showed a significant effect compared to the control at p < 0.05. The study concluded that ZnNPs can contribute to enhancing the resilience of wheat to salt stress.
Proteomic signaling of dual specificity phosphatase 4 (DUSP4) in Alzheimer’s disease
Authors: Wang Erming , Pan Allen L. , Bagchi Pritha , Ranjaraju Srikant , Seyfried Nicholas T. , Ehrlich Michelle E. , Salton Stephen R. , Zhang Bin 
Published: October 2023

Abstract
DUSP4 is a member of the DUSP (Dual-Specificity Phosphatase) subfamily that is selective to the mitogen-activated protein kinases (MAPK) and has been implicated in a range of biological processes and functions in Alzheimer’s disease (AD). In this study, we utilized stereotactic delivery of adeno-associated virus (AAV)-DUSP4 to overexpress DUSP4 in the dorsal hippocampus of 5xFAD and wildtype (WT) mice, then used mass spectrometry (MS)-based proteomics along with label-free quantification to profile the proteome and phosphoproteome in the hippocampus. We identified patterns of protein expression and phosphorylation that are modulated in 5xFAD mice and examined the sex-specific impact of DUSP4 overexpression on the 5xFAD proteome/phosphoproteome. In 5xFAD mice, a substantial number of proteins were up- or down-regulated in both male and female mice in comparison to age and sex-matched WT mice, many of which are involved in AD-related biological processes, such as the activated immune response or suppression of synaptic activities. Upon DUSP4 overexpression, significantly regulated proteins were found in pathways that were suppressed, such as the immune response, in male 5xFAD mice. In contrast, such a shift was absent in female mice. For the phosphoproteome, we detected an array of phosphorylation sites that are regulated in 5xFAD compared to WT, and are modulated by DUSP4 overexpression in each sex. Interestingly, the changes in 5xFAD- and DUSP4-associated phosphorylation occurred in opposite directions. Strikingly, both the 5xFAD- and DUSP4-associated phosphorylation changes were found for the most part in neurons, and play key roles in neuronal processes and synaptic function. Site-centric pathway analysis revealed that both the 5xFAD- and DUSP4-associated phosphorylation sites were enriched for a number of kinase sets in female, but only a limited number of sets of kinases in male mice. Taken together, our results suggest that male and female 5xFAD mice respond to DUSP4 overexpression via shared and sex-specific molecular mechanisms, which might underly similar reductions in amyloid pathology in both sexes, while learning deficits were reduced in only females with DUSP4 overexpression. Finally, we validated our findings with the sex-specific AD-associated proteomes in human cohorts and further developed DUSP4-centric proteomic network models and signaling maps for each sex.
Prevention as a Game-Theoretical Simulation in Bayesian Equilibria and Control through Real- World Data from Vaccination Monitoring
Authors: Humbsch Philipp 
Published: October 2023

Abstract
Background: Game theory is an economic model used to understand social conflict situations, identify problems, and propose solutions. In the field of medicine, which involves a tension between resource orientation and medical necessity, certain issues can benefit from game-theoretical models, particularly when social conflict situations influence the actions of public health services. Therefore, it is a possible consideration to view questions related to the COVID-19 pandemic from a game-theoretical perspective.
Method: A literature review of game theory applied to medical issues was conducted to develop a model of dynamic equilibriums concerning the question of compulsory vaccination. This model was then evaluated using freely available data from the European Centre for Disease Prevention and Control (ECDC) and the Robert Koch Institute (RKI).
Results: It was found that when preventive measures allow citizens some form of choice, they tend to make a decision with a constant probability (dynamic equilibrium). This results in a decreasing proportion of individuals continuously opting for the preventive measure, including vaccinations. Regarding vaccinations, it was observed that regardless of the existence of compulsory vaccination, there can be an asymptotic decline in the proportion of those choosing to get vaccinated within the total population, though this decline was practically unobservable in the case of SARS-CoV-2 basic immunization.
Conclusion: With all factors determining the decision for or against a preventive measure held constant, the probability remains constant with each repetition. This suggests that both the initial probability and the number of repetitions are crucial for the success of a preventive measure (e.g., basic immunization). In the case of COVID-19 vaccinations, unlike many other recommended vaccinations, it was possible to convey to the public that basic immunization consists of two doses, which increased the proportion of individuals getting the second dose. Compulsory vaccination for a single-dose vaccination that does not require boosting for a lifetime can fulfill its purpose according to this reasoning. However, compulsory vaccination for a vaccination that requires ongoing repetition cannot fulfill its purpose as the proportion of those choosing to get vaccinated decreases with each repetition. Public health decision-makers should consider this when contemplating preventive measures.
Peritumor mucosa in advanced laryngeal carcinoma exhibits an aberrant proangiogenic signature distinctive from the expression pattern in adjacent tumor tissue
Authors: Kyurkchiyan Silva G , Stancheva Gergana , Petkova Veronika , Panova Stiliana , Hadzhiev Yulian , Dobriyanova Venera , Stancheva Iglika , Kaneva Radka P , Popov Todor M 
Published: October 2023

Abstract
Field cancerization theory is an important paradigm in head and neck carcinoma as its oncological repercussions affect treatment outcomes in diverse ways. The aim of this study is to assess the possible interconnection between peritumor mucosa and the process of tumor neoangiogenesis. Sixty patients with advanced laryngeal carcinoma were enrolled in the study. The majority of patients express a canonical HIF-upregulated proangiogenic signature with almost complete predominancy of HIF-1α overexpression and normal expression levels of the HIF-2α isoform. Remarkably, more than 60% of the whole cohort exhibit a HIF-upregulated proangiogenic signature also in peritumoral benign mucosa. Additionally, the latter subgroup has a distinctly shifted phenotype towards HIF-2α upregulation compared to the one in tumor tissue, i.e., a tendency towards a HIF-switch is observed in contrast to the dominated by HIF-1α tumor phenotype. ETS-1 displays stable and identical significant overexpression in both proangiogenic phenotypes present in tumor and peritumoral mucosa. In the current study, we report for the first time the existence of an abnormal proangiogenic expression profile present in the peritumoral mucosa in advanced laryngeal carcinoma when compared to paired distant laryngeal mucosa. Moreover, we describe a specific phenotype of this proangiogenic signature that is significantly different from the one present in tumor tissue as we delineate both phenotypes, quantitively and qualitatively. This finding is per se cancer heterogeneity that extends beyond the “classical” borders of the malignancy and is proof of a strong interconnection between field cancerization and one of the classical hallmarks of cancer – the process of tumor neoangiogenesis.
Association between vitamin C in serum and trouble sleeping: evidence from NHANES 2017-2018
Authors: Wang Shihong , Lai Fengxia , Zhou Jiyu , Chen Hao , Yu Haibing , Kong Danli , Ding Yuanlin 
Published: October 2023

Abstract
Background
Vitamin C is an important micronutrient for human. Assocision between vitamin C and trouble sleeping was less studied. Therefore, the purpose of this study was to investigate the possible link between vitamin C in serum and trouble sleeping.
Methods
The cross-sectional data was derived from the National Health Nutrition and Examination Survey (NHANES, 2017–2018). We used multivariate logistic regressions to examine the possible link between vitamin C in serum and trouble sleeping, and then a subgroup analysis was performed. Moreover, the non-linear relationship between vitamin C in serum and trouble sleeping was further detected using a restricted cubic spline(RCS) model.
Results
A total of 4277 participants were included in the study. After adjusting all potential confounders, the results of multivariate logistic regression showed the signifcant association between vitamin C in serum and trouble sleeping(OR = 0.790; 95%CI = 0.680 ~ 0.918). The signifcant association was also found in female(OR = 0.694; 95%CI = 0.569 ~ 0.845), age ≤ 65 years(OR = 0.746; 95%CI = 0.616 ~ 0.904), other race(OR = 0.557; 95%CI = 0.369 ~ 0.841) and in participants with diabetes(OR = 0.692; 95%CI = 0.503 ~ 0.951) or high cholesterol level(OR = 0.728; 95%CI = 0.579 ~ 0.916). In addition, the RCS model demonstrated the signifcant non-linear relationship between vitamin C in serum and trouble sleeping(P value of nonlinear = 0.0127).
Conclusions
The cross-sectional population based study indicates that vitamin C in serum is a protective factor for trouble sleeping. Nevertheless, more studys are needed to further confrm our fndings.