bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple‐negative breast cancer
Authors: Pascual‐Reguant Laura , Serra‐Camprubí Queralt , Datta Debayan , Cianferoni Damiano , Kourtis Savvas , Gañez‐Zapater Antoni , Cannatá Chiara , Espinar Lorena , Querol Jessica , García‐López Laura , Musa‐Afaneh Sara , Guirola Maria , Gkanogiannis Anestis , Miró Canturri Andrea , Guzman Marta , Rodríguez Olga , Herencia‐Ropero Andrea , Arribas Joaquin , Serra Violeta , Serrano Luis , Tian Tian V , Peiró Sandra , Sdelci Sara 
Published: November 2023

AbstractTriple‐negative breast cancer (TNBC) often develops resistance to single‐agent treatment, which can be circumvented using targeted combinatorial approaches. Here, we demonstrate that the simultaneous inhibition of LOXL2 and BRD4 synergistically limits TNBC proliferation in vitro and in vivo. Mechanistically, LOXL2 interacts in the nucleus with the short isoform of BRD4 (BRD4S), MED1, and the cell cycle transcriptional regulator B‐MyB. These interactions sustain the formation of BRD4 and MED1 nuclear transcriptional foci and control cell cycle progression at the gene expression level. The pharmacological co‐inhibition of LOXL2 and BRD4 reduces BRD4 nuclear foci, BRD4‐MED1 colocalization, and the transcription of cell cycle genes, thus suppressing TNBC cell proliferation. Targeting the interaction between BRD4S and LOXL2 could be a starting point for the development of new anticancer strategies for the treatment of TNBC.
<i>DSG4</i> Gene Variants as a Cause of Hypotrichosis in the Child with Severe Atopic Dermatitis: Clinical Case
Authors: Murashkin Nikolay N. , Epishev Roman V. , Dubenko Olesya D. , Materikin Alexander I. , Opryatin Leonid A. , Ivanov Roman A. , Savelova Alena A. , Leonova Maria A. 
Published: November 2023

Background. Hypotrichosis is a heritable form of alopecia that causes almost complete scalp hair loss in childhood. The diagnosis is typically established according to medical history and clinical picture. Genetic testing is an additional diagnostic method that allows to establish “hypotrichosis” in cases of ambiguous or subtle clinical signs of the disease, as well as to perform differential diagnosis of this condition with others commonly associated with atopic dermatitis (focal and diffuse alopecia). Clinical diagnosis of hypotrichosis in patients with severe dermatosis is challenging.Clinical case description. The girl, aged 2 years 4 months, was hospitalized with complaints on extended rash on the body with severe itching, and changes in hair thickness, quality of hair shaft, and hair loss. Severe atopic dermatitis and hypotrichosis were diagnosed. The diagnosis of atopic dermatitis was established clinically (SCORAD — 65), the diagnosis of hypotrichosis (type 6, monilethrix-like hypotrichosis) was confirmed via molecular genetic testing (nucleotide variant c.699C>T in exone 13 of the DSG4 gene (OMIM # 607892)). Patient's mother, brother, and two sisters were diagnosed with the nucleotide variant chr18:31409487C>T in heterozygous state in the DSG4 gene. Mother and brother had no clinical manifestations, while both sisters had mild scalp hair damage, as well as eyebrows and eyelashes, since birth. There were no clinical manifestations of atopic dermatitis in parents and other children in the family.Conclusion. Hypotrichosis clinical signs can be subtled, smoothed, or imitate the symptoms of other comorbid conditions, especially in patients with comorbidities or severe dermatosis. Also worth noting is that changes in certain genes can aggravate the course of atopic dermatitis. In this clinical case, mutation in the DSG4 gene leads to epidermal barrier failure by disrupting the synthesis of desmosomes transmembrane components. Thus, geneticist consultation and genetic testing (search for changes in certain genes) are crucial in such cases.
TNF-α Inhibitor-Induced Psoriasis and Psoriatic Alopecia in Adolescent with Ulcerative Colitis: Clinical Case
Authors: Ambarchyan Eduard T. , Ivanchikov Vladislav V. , Arakelyan Anna L. , Surkov Andrey N. , Kuzminova Anastasia D. , Bessonov Evgeny E. , Komarova Elena V. 
Published: November 2023

Background. Genetically engineered biological therapy has revolutionized the treatment of many chronic inflammatory diseases. It often allows to achieve significant clinical effect and improve the patient's quality of life. However, sometimes it leads to adverse events, and physicians encounter them more often. One of such side effects is paradoxical psoriasis (PP) that can be revealed during the therapy with tumor necrosis factor alpha (TNF-α) inhibitors. PP is the debut or exacerbation of pre-existing psoriasis.Clinical case description. Patient A., 17 years old, has suffered from ulcerative colitis since 2020; biological therapy with infliximab has been initiated in March 2022. Numerous rashes were revealed, as well as development of confluent alopecia foci on the background of scalp psoriatic damage, during the next hospitalization in December 2022. The patient was examined by dermatologist; diagnosis of PP was established according to the clinical picture and medical history. Skin rashes progressed and ulcerative colitis worsened (diarrheal syndrome, fecal calprotectin levels increased up to 526 μg/g) after cessation of infliximab therapy. Biological therapy with the inhibitor IL-12/23 (ustekinumab) was initiated due to the aggressive cutaneous pathological process and the aggravation of inflammatory bowel disease (IBD) symptoms. The gradual regression of rashes with the restoration of scalp hair growth and ulcerative colitis clinical and laboratory remission were noted during the treatment.Conclusion. PP is a rare complication that develops during therapy with TNF-α inhibitors, and it is most often observed in patients with IBD. In our case there was aggressive course of psoriasis with severe scalp lesion and hair loss (it is specific type of lesion in such patients). The ustekinumab, inhibitor IL-12/23, treatment efficacy correlates with the literature data on this drug successful use in both nosologies. Ustekinumab can be a first-line therapy in such pediatric patients. This clinical case is the first case in the Russian literature on effective management of PP and ulcerative colitis with ustekinumab in children.
Linear IgA Dermatosis in a Child: Clinical Case
Authors: Murashkin Nikolay N. , Materikin Alexander I. , Epishev Roman V. , Leonova Maria A. , Opryatin Leonid A. , Ivanov Roman A. , Savelova Alena A. , Katunina Oksana R. , Kulikov Kirill A. , Danielyan Daniel R. 
Published: November 2023

Background. Linear IgA dermatosis is a rare autoimmune bullous disease characterized by vesiculo-bullous subepidermal lesions (affecting the skin and mucous membranes) and by linear homogeneous IgA deposition in the epidermis basal membrane.Clinical case description. Clinical case of linear IgA dermatosis in children is presented. Clinical data assessment, histological examination of the skin biopsy (to determine the depth of bullous), immunofluorescent examination (to reveal IgA deposition in the epidermis basal membrane) are crucial for reliable disease diagnosis according to the studies. Altogether it helps to establish the final diagnosis and determine the patient's management. Dapsone is the first-line treatment for this disease, it has proven to be an effective and safe medication.Conclusion. This case of linear IgA dermatosis is of concern due to disease severity and its rarity in clinical practice. Differential diagnosis is rather complicated and clinically requires high-tech research methods. Only immunofluorescent examination allows to diagnose linear IgA dermatosis accurately. Lesions' regression was achieved due to systemic therapy with dapsone (1.8 mg/kg/day).
Features of Cell-Mediated Immunity in Children with Congenital Ichthyosis and Their Role in the Pathogenetic Management
Authors: Avetisyan Karine O. , Murashkin Nikolay N. , Makarova Svetlana G. , Petrichuk Svetlana S. , Kuptsova Daria G. 
Published: November 2023

Background. Congenital ichthyoses (CIs) are a heterogeneous clinical-etiological group of genodermatoses. Typical clinical symptoms of this disease, regardless of the form, are generalized erythroderma, peeling, itching, hyperkeratosis, severe structural and functional disorders of the epidermal barrier, other organs and systems. Patients have an extremely low quality of life due to changes in appearance, discomfort, constant disease symptoms. Thus far, there are no effective treatment methods for ichthyosis. That is why scientific search for new therapies is the topical issue in pediatrics and pediatric dermatology.Objective. The aim of the study is to examine the cell-mediated immunity state in patients with CI via assessment of the pattern of lymphocyte subpopulations in peripheral blood. The research was conducted to study the content of the main and small lymphocyte subpopulations in 86 patients with established diagnosis of CI aged from 1 month to 18 years. The diagnosis was made according to the clinical data and the results of molecular genetic testing. Comparative analysis of blood immunological indicators in children with CI and in patients with other immunemediated chronic dermatoses: atopic dermatitis (AD; n = 68) and psoriasis vulgaris (n = 55).Methods. The level of T lymphocytes, T helpers (Th), cytotoxic T lymphocytes (Tc), B lymphocytes, NK cells, Treg-cells (Treg), activated T helpers (Thact), Th17 lymphocytes in peripheral blood was evaluated via flow cytometry using monoclonal antibodies. Statistical analysis was performed via Statistica 10.0. Differences between the groups were assessed via Mann-Whitney non-parametric test, differences were considered significant at p < 0.05.Results. A significant increase of activated T-helpers level in peripheral blood was revealed in patients with CI and psoriasis compared to children with AD (p < 0.001), as well as an increased levels of B-lymphocytes and Treg in children with CI (p < 0.05).Conclusion. Children with CI have shown some features of cell-mediated immunity such as: pathological activation of Th lymphocytes, impaired terminal differentiation of naive CD4+ cells to Thact, Treg, Th17 lymphocytes and their proliferation. Comparative analysis of mentioned immunological indicators in children with CI, psoriasis and AD has shown comparable results of increased Thact lymphocytes levels in patients in CI and psoriasis groups. This results open up potential of using immunobiological drugs of psoriasis target therapy within the new management strategy for children with CI.
Prebreeding studies of leaf rust resistant <i>Triticum aestivum/T. timopheevii</i> line L624
Authors: Sibikeev S. N. , Adonina I. G. , Druzhin A. E. , Baranova O. A. 
Published: November 2023

Triticum timopheevii Zhuk. attracts the attention of bread wheat breeders with its high immunity to the leaf rust pathogen. However, introgressions from this species in Triticum aestivum L. are little used in practical breeding. In the presented study, the agronomic value of T. aestivum/T. timopheevii line L624 was studied in comparison with the parent cultivars Saratovskaya 68, Dobrynya and the standard cultivar Favorit during 2017–2022. Introgressions from T. timopheevii in L624 were detected by the FISH method with probes pSc119.2, pAs1 and Spelt1, as well as micro satellite markers Xgwm312, Xgpw4480 and Xksum73. Translocations of 2AS.2AL-2AtL and on 2DL were detected as well. Line L624 is highly resistant to Puccinia triticina both under the background of natural epiphytotics and under laboratory conditions. PCR analysis with the DNA marker of the LrTt1 gene (Xgwm312) revealed that it is not identical to the Lr gene(s) in L624. According to a five-year study, the grain yield of L624 was, on average, higher than that of Favorit and Dobrynya, but lower than that of Saratovskaya 68. Line L624 had a lower weight of 1000 grains than the recipients, and was at the same level with the standard cultivar Favorit. Introgressions from T. timopheevii in L624 increased the grain protein content by comparison with Saratovskaya 68 and Favorit, but it was at the same level as in Dobrynya. As for parameters of flour and bread, L624 was not inferior to the recipient cultivars, but by volume and porosity of bread, it surpassed Saratovskaya 68. Moreover, L624 surpassed Favorit by the elasticity of the dough, the ratio of the elas ticity of the dough to the extensibility and the strength of the flour. Thus, the results obtained suggest that introgressions in chromosomes 2A and 2D in L624 do not impair baking properties.
A Comparative	Assessment of Driving Behaviours at Three Sites
Authors: Marsden G. R. , McDonald M. , Brackstone M. 
Published: November 2023

Many Intelligent Transport Systems (ITS) that are now under intensive investigation have been designed or assessed with a particular target consumer group in mind. In order for these (and many other) systems to be successfully used around the globe however, some appreciation will be necessary of how behaviours, and hence uses and impacts, may change between different sites and between different countries. This article reports on an instrumented vehicle study designed to assess differences in Motorway driving behaviour between three sites, one in the U.K., one in France and one in Germany. Substantial differences were found between typical car-following headways and time to collisions observed at the sites. The experiment also confirmed that many people drive well below the current recommended safe following distances. It has not been possible through this study to determine why the differences exist between the sites, nor whether they are country specific. However, the findings are important in establishing that significant differences do exist. These differences could impact on the effectiveness of roadside telematics systems as well as for the design of in-vehicle Advanced Vehicle Control and Safety Systems.
A Differential Game Modeling Approach to Dynamic Traffic Assignment and Signal Control
Authors: Li Zhenlong , Shi Songquan 
Published: November 2023

This paper addresses a theoretical issue related to combined dynamic traffic assignment and signal control under conditions of congestion through a brief review of previous research and the discussion on interaction between dynamic traffic assignment and signal control. The dynamic characteristics of the interaction are approached using a differential game modeling approach here to formulate the decision-making process for solving the problem inherent in this combination. Specifically, the combined dynamic traffic assignment and signal control problem is formulated as a leader−follower differential game, where a leader and multiple followers engage interactively to finding optimal strategies under the assumption of an openloop information structure. Discretization in time is used to find a numerical solution for the proposed game model, and a simulated annealing algorithm is applied to obtain optimal strategies. Finally, a simulation study is conducted on a simple traffic network in which numerical results demonstrate the effectiveness of the proposed approach.
Urban Transport Sustainability
Authors: Hayashi Yoshitsugu , Doi Kenji , Yagishita Masaharu , Kuwata Masako 
Published: November 2023

The paper describes the possible direction of Asian EST, starting with an overview of recent trends and problems in transport systems within Asia, policy practices are also introduced. It was found that although the current situation and trend of transport in the Asian region presents a diverse picture stemming from geographical differences and levels of economic development, most Asian countries share similar concerns about transport-related problems, namely severe congestion, air and noise pollution,. Common patterns can be identified behind these problems, which include: a)large increases in traffic demand, resulting from rapid economic growth, urbanization, and motorisation, b)poor control of vehicle emissions and the absence of effective inspection and maintenance systems, c)lack of adequate and appropriate infrastructures, and d)poor coordination of transport and land-use policies. In the current situation, insufficient understanding of environmental deterioration mechanisms delay the implementation of necessary policy measures in many Asian countries. Each country should find the best way to achieve EST as fast as possible by learning from others’ experiences. Existing good examples within the region, such as intermediate public transport systems and electric road pricing, can provide a strong foundation for this purpose.
Assessment of UK Land-Use and Transport Strategies Using Land-Use/Transport Interaction Modelling
Authors: Simmonds David 
Published: November 2023

This paper considers the contributions to economic assessment that have been achieved in a series of UK studies which have used applications of the DELTA package as the landuse/ economic component of a dynamic land-use/transport interaction model. There are three main sections to the paper. The first examines the use of these models as ways of integrating knowledge about spatial and social change for the purpose of examining alternative land-use and transport strategies. The second reviews some of the results obtained from recent projects in relation to current debates about the impacts of transport change. The third considers progress and outstanding issues in relation to the formal appraisal of the costs and benefits arising from transport interventions. The concluding section comments on some of the remaining issues to be dealt with in using these modelling and appraisal methods in relation to questions of sustainability.
LUTI modelling in the Netherlands
Authors: Schoemakers Arnout , van der Hoorn Toon 
Published: November 2023

This paper presents the use of the Dutch LUTI model TIGRIS in the planning process. TIGRIS is a long-term, incremental, time-based interaction and allocation model for land use, mobility, congestion and accessibility. In TIGRIS accessibility is described as a location factor for land-use that generates mobility. This increased mobility leads to congestion and changes in accessibility, and afterwards to new changes in land use. The model is used for regional and national forecasting, and follows a learning-by-doing approach.

In this paper four applications of TIGRIS are described with a focus on assessing impacts and the role in the planning process.

Because TIGRIS was originally developed as a sketch planning model with limited detail and the calibration on the basis of empirical data was rather limited, a new version, TIGRIS XL, is currently being developed.
Port call information sharing and inter-organizational relationships: an exploration
Authors: Nikghadam Shahrzad , Rezaei Jafar , Tavasszy Lori 
Published: November 2023

Ports have been taking major steps to improve information sharing for supporting their port call services. Despite the strong aspiration for this improvement, progress is still slow. In this paper, we argue that the progress is hampered by the challenges of an inter-organizational nature. To explore this argument, we conceptualize the link between inter-organizational relationships and information sharing in ports. We use and operationalize the partnership model, which was originally proposed for the supply chains, for the port context. We test the applicability of the model for the port of Rotterdam. Data was collected using desk research, expert interviews and surveys amongst all port actors. We find that potential for inter-organizational relationships varies substantially between the port actors, which implies an unequal potential for information sharing between them. While some actors show potential for strong relationships, in which two-way frequent exchange of information is supported, others could only support basic, occasional, or unbalanced information sharing. Therefore, it is unrealistic to assume that the information can be uniformly exchanged between all the actors. Instead, we suggest tailored information-sharing strategies that fit to actors’ business interests and characteristics. We conclude that this salient aspect of inter-organizational relationships between port actors should be acknowledged and considered for the effective design of port development strategies.
Variation in Antimicrobial Activity and Seed Storage Proteins in Three Species of the Medicinal Plant Alstonia
Authors: Kumari Jyoti , Tanuja Tanuja 
Published: November 2023

The family Apocynaceae comprises three species of the substantially important Alstonia plant, viz., A. scholaris, A. venenata, and A. macrophylla. The investigation of proteins contained within seeds, that has the potential to provide both precise details and a structural basis for characterizing diversity. The utilization of an electrophoretic technique for protein analysis has been observed in recent scholarly investigations. This implies that certain protein bands exhibit variability, with their presence or absence being detected across different seed arrangement levels in the gel. Furthermore, this implies that the protein bands have been segregated into distinct categories. A study was conducted to analyze the seed storage protein profiles of three distinct Alstonia species through the application of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The resolution of the seed storage protein of the Alstonia species using 15% SDS was found to alter the banding pattern of the polyacrylamide gel. The SDS-PAGE analysis revealed differential up regulation of proteins across distinct bands. Throughout the examination, it was determined that the three aforementioned species exhibited acommon band, in addition to a protein with a molecular weight of 34 kDa. The number of protein bands attached to A. venenata was the highest (ten bands), while the number of protein bands that adhered to A. scholaris was the lowest (five bands). Further, powdered leaves of A. scholaris, A. venenata, and A. macrophylla were investigated for antibacterial and the results, A. macrophylla leaf powder is most effective against Staphylococcus aureus, followed by A. venenata and A. scholaris at 1 μg/ mL. In addition, the findings support the inference that the A. scholaris leaf powder effectively inhibited the growth of Aspergillus niger. A. venenata and A. macrophylla, both at 1μg/mL, are, nonetheless, also effective against A. niger. A. macrophylla has the largest zone of inhibition (11.5 mm) against A. niger.