bluuebunny/crossref_metadata_2025_split huggingface dataset:
50 Individual Precision Feeding can Greatly Reduce Global Warming, Eutrophication, and Acidification Environmental Impacts
Authors: Llorens Berta , Pomar Candido , Goyette Bernard , Rajagopal Rajinikanth , Andretta Ines , Latorre María Ángeles , Remus Aline 
Published: October 2023

Abstract
Nitrogen and phosphorus excretion are among the primary potential sources of environmental contamination in growing-finishing pig operations. Nutrient excretion can be reduced by feeding pigs with daily tailored diets to their estimated nutrient requirements using individual precision feeding (IPF) techniques. The environmental impact of moving from conventional group 3-phase-feeding (CON) to IPF systems in Québec, Canada, was evaluated in this study using life-cycle analysis. A cradle-to-farm gate life-cycle analysis was conducted using Simapro software (v. 8.0.3.14; PRE Consultants, Amersfoort, The Netherlands). The model included inputs and outputs of each sub-phase: raw materials/feedstuffs production, feed mill processing transport, animal rearing (maternity, weaning, and fattening units), and manure spreading in all livestock productions. All feed ingredients originated from Quebec (Montérégie region) and agricultural practices were simulated using real management data from an average farm in Quebec. Based on observed pig growth data, the CON and IPF systems were compared in the growing-finishing phase (Andretta et al., 2016). The IPF diets were obtained by blending two feeds (i.e., A and B), while CON diets were formulated according to the ones used by the industry. The evaluated impact categories were global warming (GW), eutrophication (EU), and acidification (AC). The functional unit was 1 ton of feed at the feed mill gate, and 1 ton of pig live weight at the farm gate for finished pigs. A Monte Carlo analysis was performed to determine the uncertainty of the growth performance results. Feeding programs were compared with ANOVA. Corn was the ingredient with greater GW and AC impacts, therefore, diets with greater corn content were those with greater impacts in these categories. Feed B, which contained 83.2% of corn, resulted in impacts of 570 kg of CO2 eq., 8.21 kg SO2 eq. and 6,27 kg PO4 eq. Diets with greater EU impact were those with a greater percentage of soybean meal. Feed A contained 25.4% of this ingredient and impacted 554 kg of CO2 eq., 6,84 kg SO2 eq. and 7.05 kg PO4 eq. CON diets had environmental impacts between those of feeds A and B. Compared with CON, IPF decreased GW by 5.1%, AC by 14.2% and EU by 12.2%. IPF significantly reduced the environmental impact in all categories due to the more efficient use of nutritional resources. IPF is an effective alternative to improve the sustainability of growing-finishing pig operations in Québec and likely other regions using corn and soybean-based diets.
54 Impact of Ileal Indigestible Protein on Nitrogen Excretion and Fecal Microbiota may be Greater Compared with Total Protein Concentration of Diets in Growing Pigs
Authors: Sung Jung Yeol , Johnson Timothy A , Ragland Darryl , Adeola Olayiwola 
Published: October 2023

Abstract
The effect of changes in dietary ileal indigestible protein concentration may be more influential in the amount of nitrogen flowing to the large intestine compared with changes in total dietary protein concentration. For this reason, we hypothesized that an increase in dietary ileal indigestible protein concentration induces an increase in hindgut nitrogen utilization and nitrogen excretion and a shift in fecal microbiota in growing pigs, when compared with pigs given a high total protein diet. Three diets were prepared: 1) standard protein diet based on corn and soybean meal, 2) high-indigestible protein diet in which autoclaved soybean meal replaced normal soybean meal in the first diet, and 3) high protein diet where the inclusion rate of soybean meal was greater than that of the other diets. T-cannulated barrows (n = 18; initial BW = 63.4 ± 8.0 kg) were allotted to the 3 diets in a randomized complete block design with BW as a blocking factor. Pigs were individually housed in pens and the experiment lasted for 23 days. On d 7 and 21, fecal samples were collected by rectal massage for microbiota analysis. Grab samples of feces were collected on d 20 and 21, and ileal digesta were collected on d 22 and 23 for the determination of energy and nitrogen utilization. Energy and nitrogen utilization data were analyzed using the MIXED procedure of SAS using a pig as the experimental unit and a model that included diet as a fixed variable and block as a random variable. For microbiome analysis, R was used. Alpha and beta diversity metrics were analyzed and the difference in abundance of the genus was determined using the DESeq2 function. The high-indigestible protein diet achieved decreased apparent ileal digestibility of nitrogen and resulted in greater ileal indigestible nitrogen concentration (P &lt; 0.05). Apparent total tract digestibility of nitrogen was less (P &lt; 0.05), and correspondingly fecal nitrogen concentration and daily fecal nitrogen output were greater (P &lt; 0.05) in the high-indigestible protein diet. Apparent post-ileal digestibility and hindgut disappearance of nitrogen and gross energy were the greatest (P &lt; 0.05) in the high protein diet. Beta diversity metrics of feces in the high-indigestible protein diet on d 21 were different (q &lt; 0.05) from those in the other two diets, which indicates a shift in fecal microbiota. According to the DESeq2 results, the direction of the microbiota shift induced by the high-indigestible protein diet may have reduced hindgut fiber utilization. In conclusion, an increase in dietary ileal indigestible protein increased nitrogen excretion and shifted fecal microbial communities but did not increase hindgut nitrogen utilization.
51 Evaluation of the Effects of a Single Intranasal Dose of Essential oil Spray on the Nasopharyngeal Microbiota of Feedlot Cattle: A Pilot Study
Authors: Magossi Gabriela , Schmidt Kaycie N , Holman Devin B , Winders Thomas , Carlson Zachary , Underdahl Sarah R , Swanson Kendall C , Amat Samat 
Published: October 2023

Abstract
Five essential oils (EO) were previously characterized in vitro and identified as candidates for the development of an intranasal EO spray to inhibit the colonization and proliferation of bovine respiratory disease (BRD) pathogens, especially at feedlot entry when cattle are most susceptible to BRD. In the present pilot study, these EO were evaluated for their potential to reduce BRD pathogens and to modulate nasopharyngeal microbiota in feedlot cattle. Angus-crossbred calves [n = 40; 7to 8 months old; initial BW = 284 ± 5 kg (SE)] received either an intranasal EO spray consisting of ajowan, thyme, fennel, cinnamon leaf, and citronella (0.025%, vol/vol per EO) suspended in PBS (3 mL per nostril) or PBS (Control) on day (d) 0. Deep nasopharyngeal swabs were collected on d -1 (24 h before EO administration), 1, 2, 7, 14, 28, and 42, and processed for microbiota characterization using 16S rRNA gene. The calves that received EO had similar feed intake (P = 0.62) and average daily gain (P = 0.37) than control calves. Overall bacterial concentration estimated by qPCR was greater in control (P =0.049) than EO group, with EO group having reduced bacterial concentration on d 2 (P = 0.01) and d 7 (P=0.07). Sequencing results revealed that overall community structure of nasopharyngeal microbiota was different between EO and control calves (PERMANOVA: R2 = 0.01, P = 0.02), and was strongly affected by time (R2 = 0.1, P = 0.001). Within individual sampling day, the effect of EO on community structure was only detected on d 1 (R2 = 0.05, P = 0.03). Microbial richness (observed ASVs) and diversity (Shannon and Inverse Simpson) were not different between the two groups (P ≥ 0.17). The effect of EO on the most dominant phyla was observed (P ≤ 0.05) with Proteobacteria (d14), Firmicutes (d1, 14), Actinobacteriota (d14), and Bacteroidota (d2, 14). Among the predominant genera: Moraxella, Ralstonia, Mycoplasma, Mannheimia, Filobacterium, Pseudomonas, Pasteurella, and Corynebacterium, the abundance of Mannheimia (4.34% vs. 10.4%) and Filobacterium (2.53% vs. 11.9%) were reduced (P &lt; 0.05) by EO on d 2. In control calves, Mannheimia was enriched by 3.9-fold (P &lt; 0.05) from d -1 (2.66%) to d 2 (10.4%), whereas it remained constant in EO calves (P &gt; 0.05) between these days (2.23% and 4.34%). Corynebacterium was also decreased (P &lt; 0.05) in EO calves on d 1 (1.06% vs. 2.58%). The BRD-associated Histophilus genus was low (mean 0.02%), so EO effect was not assessed. Overall, a single dose of intranasal EO spray modulated nasopharyngeal microbiota and reduced the abundance of Mannheimia within 48 h post administration while having no effect on feed intake and daily gain. Our study highlights that nasally dosing EO may modulate respiratory microbiota and potentially mitigate bovine respiratory disease in finishing cattle.
193 Creatine Monohydrate on In Vitro Cultivated Bovine Cells Leading to Guanidinoacetic Acid Supplementation In Vivo
Authors: Eckhardt Erika P , Kim WonSeob , Kim Jongkyoo 
Published: October 2023

Abstract
Guanidinoacetic acid (GAA) is an amino acid-like compound that acts as a precursor to creatine (CRE) when either made in the body or digested. Creatine is directly used by skeletal muscle tissue as an energy source with the potential to act as a growth-promoting agent. The study objectives were to evaluate the effects of various CRE concentrations on bovine satellite cells for the rate of proliferation and myogenic differentiation. Satellite cells (SC) were isolated from the Longissimus muscle of 3-month-old Holstein bulls (n = 5). Cells were seeded at a density of 1 × 104 cells/mL on 96-well plates and incubated with 0 (CON), 2.5, 5, 7.5, and 10 mM of CRE. The absorbance rate was measured at 0, 24, 48, 72, and 96 h of incubation. Cells for gene expression were plated on 6-well plates at a density of 1 × 105 in 10% FBS/DMEM. Following 24 h of adhesion, SC were incubated with 5 and 7.5mM CRE for 48 h. After which, cells were harvested after 48 h for Pax3, Pax7, and Myf5 mRNA gene expression analysis. To identify the effects of CRE during myogenic differentiation, SCs were induced myogenic differentiation. Upon reaching approximately 90% confluency, the growth medium was replaced by differentiation media (2% horse serum/ DMEM) containing 0 (CON), 5, and 7.5 mM CRE. A two-way ANOVA and Tukey's HSD test were conducted regarding cell proliferation. A one-way ANOVA and Tukey's HSD test were performed for gene expression. Interaction of treatment against time was noted (P &lt; 0.01) in Con, 2.5, 5, 7.5, and 10 mM CRE. Cell proliferation rate was influenced by CRE treatment (P &lt; 0.01). At 72 h of incubation, all CRE treatments increased cell proliferation rate compared with Con (P &lt; 0.05). At 96 h of incubation, cells treated with 2.5, 5, and 7.5 mM of CRE increased proliferation compared with Con (P &lt; 0.05). At 96 h, CRE treatment 2.5, 5, and 7.5 mM increased proliferation rate compared with 10mM CRE treated cells (P &lt; 0.05). No treatment effect was found in the mRNA gene expression of Pax7, Pax3, and Myf5 (P &gt; 0.05). Cells incubated with CRE showed no change in mRNA gene expression of IGF-1 and Myogenin (P &gt; 0.05), and a tendency was noted for S6KB1 increase expression under treatment (P = 0.0643). S6KB1 is a protein kinase that, through mTOR pathway expression, leads to an increase in cell growth, protein synthesis, and cell proliferation. Results suggest that the treatment of BSCs with CRE impacted proliferation, CRE at 5mM vs. 7.5 mM concentrations treatment of differentiated muscle cells led to a trend in altering S6KB1 gene expression.
190 Effect of Segregation in Pigs in the Nursery Period by Body Weight (Light, Medium, and Heavy) on the Productive Performance on Wean-to-Finish
Authors: Rodriguez Cristina Rodriguez , Gómez Álvaro Rojo , Ramos Diego Aguilera , Cota José Angel , Mikovich Elías , Martínez Víctor Alfonso , León Ricardo Alberto , Sánchez Luis Angel 
Published: October 2023

Abstract
A management strategy to improve growth performance at weaning is segregation of pigs in the nursery period by body weight (BW; Light, Medium, and Heavy) in an attempt to increase productive performance and decrease within pen variation in BW. However, the result of current studies indicated that this practice may not be as effective as not sorting pigs. The objective was to determine the effect on productive performance and coefficient of variation (CV) within four categories of BW: Normal (without segregation), Light, Medium, and Heavy. A total of 24 Experimental Units, (EU; 50% barrows and 50% gilts) in an Incomplete Randomized Block Design with four treatments (Trt) and six replicates per Trt. Pen was the EU and block was the weaning group. Treatments were four; Normal (without segregation; 6.35 kg BW); Light (4.45 kg BW); Medium (5.89 kg BW); and Heavy (7.15 kg BW). Pigs were weighed individually on d 21 (at the beginning of the experiment), 70, 119, 135 and 146 of life. Growth data were analyzed using PROC MEANS and GLM of SAS. Results indicated that at the beginning of the experiment (for design) BW and the CV were different (P &lt; 0.05). From weaning to d 70 Medium and Normal categories had similar BW (29.79 vs. 30.57 kg; P &gt; 0.05), respectively. Light and Heavy pigs remained unchanged in their weight categories. Interestingly, the CV at d 70 was 3.46 percentage points greater in all the groups of pigs that were regrouped by weight, in fact, the only group that reduced the CV at d 70 was the Normal (it was decreased by 3.26 percentage points). Average daily gain at day 70 was less (P &lt; 0.05) for Light (0.62 kg/d) compared with any of the other treatments (0.69 kg/d, average of the rest of the treatments). Average daily feed intake was less for Light (0.940 kg/d) followed by Medium and Normal (1.00 and 1.04 kg per day, respectively) and the Heavy which had the greatest intake (1.08 kg/d). No differences were found in removed animals and mortality (P &gt; 0.05). However, the total number of animals treated parenterally was greater in the Light and Medium (16.75 vs. 12.80, respectively; P &lt; 0.05) than the Heavy and Normal (7.83 and 7.00, respectively, P &lt; 0.05). At the end of the experiment the BW of the pigs was different between treatments (P &lt; 0.05) being the Light group pigs the least heavy (120.62 kg), followed by the Medium (128.02 kg) and Normal (131.31 kg) the treatment Heavy was the heaviest with 133.04 kg. These results suggest that segregation in nursery by BW has a negative effect on the CV.
81 Creating a More Efficient Production System (Feed Efficiency in the Real World)
Authors: Smith Zachary K , Gentry Wes 
Published: October 2023

Abstract
Modern food production is the subject of much scrutiny, especially with regard to potential environmental impact and sustainability. To accurately assess impact, both costs and benefits should be evaluated, and inputs and outputs should be standardized. Emphasis is commonly placed on efficiency when comparing production of various animal proteins. Efficiency of a process can be described as a ratio. This ratio is simply: 1) input to output or 2) output to input. The relationship of input to output such as feed input to gain (i.e., body weight, carcass weight, or retail cut weight) output is termed feed to gain (F:G) and the relationship of output to input is termed gain to feed (G:F). Production efficiency is optimal when animals are depositing lean tissue and poorest when the animal fattens and approaches chemical maturity. Because of the energy density of tissues deposited, energetic (calorie:calorie) efficiency can look very different than production (feed) efficiency. Factors related to animal health are also important as a reduction in death loss can offer a substantial boost to efficiency. Feed efficiency should be expressed on a dry matter basis. Failure to account for moisture content in a feedstuff can make feed efficiency appear poorer, especially when cattle are compared with non-ruminants, which typically consume drier diets. Should the environmental impact of beef production be lifetime or feedyard only? Much, if not all, of what a cow eats has no other value to mankind. The authors believe that the creation of human available calories from cellulose must be accounted for. For a more just comparison, efficiency could be measured using inputs that could have been used for human consumption, when making across species comparisons. With this in mind, non-ruminants are not nearly as attractive for large-scale protein production. There would also be value in evaluating efficiency of input to ideal protein output, rather than just weight gain or pounds of food produced. In that scenario plant-based foods would fare poorly. How best can cattle be used to supply the bioavailable nutrients for a growing population? Modern cattle eat large amounts of feed, get very large and have the ability to fatten and still be very efficient. Continual improvement and advancement is critical; but more meaningful assessments of efficiency are possible when the appropriate input:ouput comparisons are evaluated.
1 Microbiomes from Theory to Application
Authors: Adams Seidu , Aluthge Nirosh D , Abbas Waseem , Spangler Matt L , James Wells , Hales Kristen E , Kuehn Larry A , Oliver William T , Burkey Thomas , Miller Phillip S , Fernando Samodha C 
Published: October 2023

Abstract
Microbiome research has gained significant momentum in the last decade. Although many studies have investigated livestock microbiome to improve animal health and productivity, only a few microbiome manipulation approaches are currently used in the industry. This is partly due to the limited understanding of livestock microbiome and how such knowledge can be applied to improve animal health and performance. Additionally, the potential role of the microbiome is poorly characterized, limiting the opportunities available to improve animal health and productivity through microbiome manipulation. Here we describe and demonstrate how current microbiome theory such as “colonization history” can be utilized to establish beneficial microbiomes in livestock species. Our data suggest that the sow microbiome greatly impacts the early microbiome establishment in piglets and represents as much as 67% of the community even after 21 days following birth. This suggests early colonization provides unique opportunities to establish “beneficial” microbiomes. Additionally, we describe how microbiome-wide association studies (MWAS) can be used to quantify the microbial role in animal performance using a cattle population consisting of more than 750 animals. Finally, we describe how livestock animal models can be used to understand key concepts of microbial establishment and how such models can be used to investigate the human microbiome using germ free pig models. USDA is an equal opportunity provider and employer.
114 Effects of Zinc Supplementation in Gestating Sow Diets on Growth Performance of the Progeny from Birth to Market Reared Under Commercial Conditions
Authors: Cony Bruna S L , de Araujo Edilaine C , Martins Gabriel , Furtado Júlio , Vier Carine M , Orlando Uislei A , Lunedo Raquel , Perondi Dani , Andretta Ines 
Published: October 2023

Abstract
The objective of this study was to evaluate the effects of different Zn supplementation strategies for gestating sows on growth performance of the progeny from birth to market. Pregnant sows (n = 417) were assigned to 1 of 3 dietary treatments in a randomized complete block design based on parity and body condition. Dietary treatments consisted of: 1) Control (CON), corn- soybean meal-based diet with 125 ppm of Zn/kg diet supplied by ZnO (standard basal diet), for a total daily intake of 250 ppm Zn; 2) Partial Supplementation (PS), CON diet + 625 ppm supplemental Zn top-dressed for a total daily intake of 850 ppm Zn provided from d 80 of gestation up to farrowing; and 3) Total Supplementation (TS), CON diet + 625 ppm supplemental Zn top-dressed for a total daily intake of 850 ppm Zn provided throughout the entire gestation phase. Individual body weight was recorded for all piglets at birth (n = 6,349) and at weaning (n = 5,016). A subgroup of piglets (same gender, housed in same farm) was also individually weighed at the end of nursery phase (n = 1207) and at the end of finishing phase (n = 260). Data were assessed by analysis of variance interpreted at 5 and 10% probability levels, adjusted by the Tukey-Kramer test for multiple comparisons. Piglets born from sows whose diets were supplemented with Zn throughout the entire gestational period (TS) tended (P = 0.10) to be heavier at birth compared with piglets born from CON sows (Table 1). The offspring from TS sows tended (P = 0.09) to have greater average daily weight gains during lactation and were heavier (P = 0.04) at weaning compared with the offspring from CON sows, with the offspring from PS sows being intermediate. Although there was no evidence for differences in nursery average daily weight gain, piglets from TS sows tended (P = 0.10) to be heavier at the end of the nursery compared with piglets from CON sows, with piglets from PS intermediate. Pigs from TS sows had greater (P = 0.003) average daily weight gain during the finishing phase and were heavier (P = 0.03) at slaughter. Only animals from the treatments CON and TS were evaluated at the end of the trial because no evidence for differences (P &gt; 0.10) between CON and PS were found at the end of the nursery phase. In conclusion, Zn supplementation in gestating sow diet fed throughout the entire gestation period optimized the performance of the progeny from birth to market and should be considered as a viable prenatal intervention in commercial production systems.