bluuebunny/crossref_metadata_2025_split huggingface dataset:
Team‐based learning in Indonesian midwifery education: Implementation research
Authors: Ulfa Yunefit , Horiuchi Shigeko , Shishido Eri , Igarashi Yukari 
Published: February 2024

AbstractAimThis study aims to implement team‐based learning (TBL) and assess the impact on faculty members and students within midwifery education in Indonesia.MethodsProctor's Framework for Implementation Research serves as the guiding approach in investigating the role of implementation strategies in implementing TBL within the context of midwifery education. The RE‐AIM (Reach, Efficacy, Adoption, Implementation, and Maintenance) framework was utilized to assess the outcome.ResultsIn this study, the implementation of TBL has demonstrated significant reach, with broad participation among both faculty and students in two schools. The efficacy of TBL is evident through enhanced student knowledge, engagement and active learning. Adoption of TBL was observed in both schools, with faculty and students expressing interest and active participation. High levels of implementation fidelity were maintained, even though with challenges related to preparation and implementation. These findings suggest that TBL can be successfully integrated into midwifery education, with positive implications. Regarding maintenance, faculty members have expressed their intention to continue using TBL in various topics for future lectures.ConclusionsThe implementation of TBL in Indonesian midwifery education has shown substantial reach and efficacy. Faculty and students are highly interested in adopting TBL for future use. Despite some implementation challenges, the study suggests that TBL can be effectively incorporated with minor adjustments, emphasizing its feasibility and potential impact. This research contributes to understanding TBL's applicability in various educational settings, especially in low‐resource institutions.
The Effect of Job Loss on Bank Account Ownership
Authors: GOODSTEIN RYAN M. , KUTZBACH MARK J. 
Published: February 2024

AbstractWe estimate the effect of job loss on households’ bank account ownership using novel data: Federal Deposit Insurance Corporation (FDIC)‐sponsored biennial supplements to the Current Population Survey (CPS), linked to respondents’ work history in surrounding months constructed from the Basic Monthly CPS. Leveraging differences in the timing of unemployment spells across households, we show that job loss leads to a large decrease in the likelihood of having an account among the lower‐income, renter households we study. Job loss also leads to increased use of other products and services that might substitute for a bank account, including prepaid cards, check cashing, and money orders.
Synaptic adhesion molecule protocadherin‐γC5 mediates β‐amyloid‐induced neuronal hyperactivity and cognitive deficits in Alzheimer's disease
Authors: Su Min , Xuan Erying , Sun Xiangyi , Pan Gaojie , Li Dandan , Zheng Honghua , Zhang Yun‐wu , Li Yanfang 
Published: February 2024

AbstractNeuronal hyperactivity induced by β‐amyloid (Aβ) is an early pathological feature in Alzheimer's disease (AD) and contributes to cognitive decline in AD progression. However, the underlying mechanisms are still unclear. Here, we revealed that Aβ increased the expression level of synaptic adhesion molecule protocadherin‐γC5 (Pcdh‐γC5) in a Ca2+‐dependent manner, associated with aberrant elevation of synapses in both Aβ‐treated neurons in vitro and the cortex of APP/PS1 mice in vivo. By using Pcdhgc5 gene knockout mice, we demonstrated the critical function of Pcdh‐γC5 in regulating neuronal synapse formation, synaptic transmission, and cognition. To further investigate the role of Pcdh‐γC5 in AD pathogenesis, the aberrantly enhanced expression of Pcdh‐γC5 in the brain of APP/PS1 mice was knocked down by shRNA. Downregulation of Pcdh‐γC5 efficiently rescued neuronal hyperactivity and impaired cognition in APP/PS1 mice. Our findings revealed the pathophysiological role of Pcdh‐γC5 in mediating Aβ‐induced neuronal hyperactivity and cognitive deficits in AD and identified a novel mechanism underlying AD pathogenesis.image
Deploy the proboscis!: Functional morphology and kinematics of a novel form of extreme jaw protrusion in the hingemouth, Phractolaemus ansorgii (Gonorynchiformes)
Authors: Evans Allyson J. , Naylor Emily R. , Lujan Nathan K. , Kawano Sandy M. , Hernandez L. Patricia 
Published: February 2024

AbstractPremaxillary protrusion and the performance advantages it confers are implicated in the success of diverse lineages of teleost fishes, such as Cypriniformes and Acanthomorpha. Although premaxillary protrusion has evolved independently at least five times within bony fishes, much of the functional work investigating this kinesis relates to mechanisms found only in these two clades. Few studies have characterized feeding mechanisms in less‐diverse premaxilla‐protruding lineages and fewer yet have investigated the distinctive anatomy underlying jaw kinesis in these lineages. Here, we integrated dissection, clearing and staining, histology, micro‐CT, and high‐speed videography to investigate an isolated and independent origin of jaw protrusion in the hingemouth, Phractolaemus ansorgii, which employs a complex arrangement of bones, musculature, and connective tissues to feed on benthic detritus via a deployable proboscis. Our goals were to provide an integrative account of the underlying architecture of P. ansorgii's feeding apparatus and to assess the functional consequences of this drastic deviation from the more typical teleost condition. Phractolaemus ansorgii's cranial anatomy is distinct from all other fishes in that its adducted lower jaw is caudally oriented, and it possesses a mouth at the terminal end of an elongated, tube‐like proboscis that is unique in its lack of skeletal support from the oral jaws. Instead, its mouth is supported primarily by hyaline‐cell cartilage and other rigid connective tissues, and features highly flexible lips that are covered in rows of keratinous unculi. Concomitant changes to the adductor musculature likely allow for the flexibility to protrude the mouth dorsally and ventrally as observed during different feeding behaviors, while the intrinsic compliance of the lips allows for more effective scraping of irregular surfaces. From our feeding videos, we find that P. ansorgii is capable of modulating the distance of protrusion, with maximum anterior protrusion exceeding 30% of head length. This represents a previously undescribed example of extreme jaw protrusion on par with many acanthomorph species. Protrusion is much slower in P. ansorgii—reaching an average speed of 2.74 cm/s—compared to acanthomorphs feeding on elusive prey or even benthivorous cypriniforms. However, this reorganization of cranial anatomy may reflect a greater need for dexterity to forage more precisely in multiple directions and on a wide variety of surface textures. Although this highly modified mechanism may have limited versatility over evolutionary timescales, it has persisted in solitude within Gonorynchiformes, representing a novel functional solution for benthic feeding in tropical West African rivers.
Retrospective clinical evaluation of Q‐switched Nd:YAG laser safety and efficacy in tattoo removal: A new perspective on the Kirby–Desai scale
Authors: Egozi Ella , Toledano Ohad 
Published: February 2024

AbstractBackgroundWith nearly 50% of the population in the United States, Italy, and Sweden tattooed with at least one tattoo, the demand for its removal has risen by 32% since 2011. Traditional removal methods, such as Q‐switched (QS) laser‐based tattoo removal, can be lengthy, requiring up to 20 sessions.AimThis study presents a retrospective clinical evaluation of seven short‐pulsed QS, dual‐wavelength Nd:YAG laser, as an efficient alternative, that can potentially reduce the number of sessions needed as calculated by a founded scale.MethodsThe QS modality delivers high‐intensity pulses in four wavelengths, ideal for removing multicolored tattoos, of which two were used. We studied 11 patients who underwent 3–8 treatments (average 5.09) every 2–3 months. Each tattoo was assessed using the Kirby–Desai scale, considering the following factors: location, Fitzpatrick skin type, ink amount, layering, scarring, tissue changes, and tattoo color. Follow‐ups were conducted after 6 months and at 4–5 years following last session.ResultsThe long‐term follow‐up presented a significantly higher tattoo removal efficiency than the short‐term follow‐up (p < 0.001), indicating a sustained process of ink breakdown and elimination. Notably, the actual number of treatments were significantly lower than that predicted by the Kirby–Desai scale (average 5.09 vs. 9.9, p < 0.001). No severe adverse events were reported.ConclusionsIn conclusion, the QS Nd:YAG laser offers a safe and effective alternative for tattoo removal, requiring fewer treatments than initially expected.
Patient safety in surgical settings: A study on the challenges and improvement strategies in adverse event reporting from a nursing perspective
Authors: Gómez‐Moreno Cristina , Vélez‐Vélez Esperanza , Garrigues Ramón Marta , Rojas Alfaro Marta , García‐Carpintero Blas Eva 
Published: February 2024

AbstractAimsTo explore adverse event reporting in the surgical department through the nurses' experiences and perspectives.DesignAn exploratory, descriptive qualitative study was conducted with a theoretical‐methodological orientation of phenomenology.MethodsIn‐depth interviews were conducted with 15 nurses, followed by an inductive thematic analysis.ResultsThemes include motives for reporting incidents, consequences, feelings and motivational factors. Key facilitators of adverse event reporting were effective communication, knowledge sharing, a non‐punitive culture and superior feedback.ConclusionThe study underscores the importance of supportive organisational culture for reporting, communication and feedback mechanisms, and highlights education and training in enhancing patient safety.ImplicationsIt suggests the need for strategies that foster incident reporting, enhance patient safety and cultivate a supportive organisational culture.ImpactThis study provides critical insights into adverse event reporting in surgical departments from nurses' lived experience, leading to two primary impacts: It offers specific solutions to improve adverse event reporting, which is crucial for surgical departments to develop more effective and tailored reporting strategies. The research underscores the importance of an open, supportive culture in healthcare, which is vital for transparent communication and effective reporting, ultimately advancing patient safety.Reporting MethodThe study followed the Standards for Reporting Qualitative Research and the Consolidated Criteria for Reporting Qualitative Research guidelines.Patients or Public ContributionNo patients or public contribution.
Adultification in the context of childhood exposure to domestic violence
Authors: Haselschwerdt Megan L. , Tunkle Caroline 
Published: February 2024

AbstractObjectiveDrawing upon family systems theory, Burton's childhood adultification model, and Johnson's typology of domestic violence (DV), the objective of this qualitative study was to understand the adultification experiences of young adults who were exposed to DV while growing up.BackgroundExposure to DV negatively impacts familial dynamics, disrupting healthy boundaries between caregivers and children. Often associated with families experiencing poverty, adultification is a type of boundary infringement that places children in adult‐like roles to execute essential family tasks with potentially dangerous and developmentally harmful effects. A growing body of literature documents how youth are agentic in navigating their family dynamics and how abusive partners use children as abuse tools. However, adultification in a DV context remains understudied.MethodUsing a qualitative study design, the research team interviewed 23 college‐attending young adults with father‐mother‐perpetrated DV exposure histories who resided in the Southeastern United States. The qualitative data were analyzed using theoretical thematic analysis.FindingsWe identified five distinct yet interrelated ways in which young adults with DV exposure histories experienced adultification: intervening to protect mothers from violence, serving as mothers' emotional support system, shielding siblings from violence and conflict, caring for siblings' daily needs, and managing parents' health and well‐being. The young adults categorized as exposed to coercive controlling violence described more extensive adultification.ConclusionCentering adultification in the context of family violence provides a lens through which researchers, practitioners, and other professionals can understand how DV impacts family dynamics, including adultified children.
Unusual sources of fossil micrometeorites deduced from relict chromite in the small size fraction in ~467 Ma old limestone
Authors: Heck Philipp R. , Schmitz Birger , Ritter Xenia , Rout Surya S. , Kita Noriko T. , Defouilloy Céline , Keating Katarina , Eisenstein Kevin , Terfelt Fredrik 
Published: February 2024

ABSTRACTExtraterrestrial chrome spinel and chromite extracted from the sedimentary rock record are relicts from coarse micrometeorites and rarely meteorites. They are studied to reconstruct the paleoflux of meteorites to the Earth and the collisional history of the asteroid belt. Minor element concentrations of Ti and V, and oxygen isotopic compositions of these relict minerals were used to classify the meteorite type they stem from, and thus to determine the relative meteorite group abundances through time. While coarse sediment‐dispersed extraterrestrial chrome‐spinel (SEC) grains from ordinary chondrites dominate through the studied time windows in the Phanerozoic, there are exceptions: We have shown that ~467 Ma ago, 1 Ma before the breakup of the L chondrite parent body (LCPB), more than half of the largest (>63 μm diameter) grains were achondritic and originated from differentiated asteroids in contrast to ordinary chondrites which dominated the meteorite flux throughout most of the past 500 Ma. Here, we present a new data set of oxygen isotopic compositions and elemental compositions of 136 grains of a smaller size fraction (32–63 μm) in ~467 Ma old pre‐LCPB limestone from the Lynna River section in western Russia, that was previously studied by elemental analysis. Our study constitutes the most comprehensive oxygen isotopic data set of sediment‐dispersed extraterrestrial chrome spinel to date. We also introduce a Raman spectroscopy‐based method to identify SEC grains and distinguish them from terrestrial chrome spinel with ~97% reliability. We calibrated the Raman method with the established approach using titanium and vanadium concentrations and oxygen isotopic compositions. We find that ordinary chondrites are approximately three times more abundant in the 32–63 μm fraction than achondrites. While abundances of achondrites compared to ordinary chondrites are lower in the 32–63 μm size fraction than in the >63 μm one, achondrites are approximately three times more abundant in the 32–62 μm fraction than they are in the present flux. We find that the sources of SEC grains vary for different grain sizes, mainly as a result of parent body thermal metamorphism. We conclude that the meteorite flux composition ~467 Ma ago ~1 Ma before the breakup of the LCPB was fundamentally different from today and from other time windows studied in the Phanerozoic, but that in contrast to the large size fraction ordinary chondrites dominated the flux in the small size fraction. The high abundance of ordinary chondrites in the studied samples is consistent with the findings based on coarse extraterrestrial chrome‐spinel from other time windows.
The mechanistic basis of the membrane‐permeabilizing activities of the virulence‐associated protein A (VapA) from Rhodococcus equi
Authors: Nehls Christian , Schröder Marcel , Haubenthal Thomas , Haas Albert , Gutsmann Thomas 
Published: February 2024

AbstractPathogenic Rhodococcus equi release the virulence‐associated protein A (VapA) within macrophage phagosomes. VapA permeabilizes phagosome and lysosome membranes and reduces acidification of both compartments. Using biophysical techniques, we found that VapA interacts with model membranes in four steps: (i) binding, change of mechanical properties, (ii) formation of specific membrane domains, (iii) permeabilization within the domains, and (iv) pH‐specific transformation of domains. Biosensor data revealed that VapA binds to membranes in one step at pH 6.5 and in two steps at pH 4.5 and decreases membrane fluidity. The integration of VapA into lipid monolayers was only significant at lateral pressures <20 mN m−1 indicating preferential incorporation into membrane regions with reduced integrity. Atomic force microscopy of lipid mono‐ and bilayers showed that VapA increased the surface heterogeneity of liquid disordered domains. Furthermore, VapA led to the formation of a new microstructured domain type and, at pH 4.5, to the formation of 5 nm high domains. VapA binding, its integration and lipid domain formation depended on lipid composition, pH, protein concentration and lateral membrane pressure. VapA‐mediated permeabilization is clearly distinct from that caused by classical microbial pore formers and is a key contribution to the multiplication of Rhodococcus equi in phagosomes.
The metabolite vanillic acid regulates Acinetobacter baumannii surface attachment
Authors: Brychcy Merlin , Nguyen Brian , Tierney Guillermo Antunez , Casula Pranav , Kokodynski Alexis , Godoy Veronica G. 
Published: February 2024

AbstractThe nosocomial bacterium Acinetobacter baumannii is protected from antibiotic treatment by acquiring antibiotic resistances and by forming biofilms. Cell attachment, one of the first steps in biofilm formation, is normally induced by environmental metabolites. We hypothesized that vanillic acid (VA), the oxidized form of vanillin and a widely available metabolite, may play a role in A. baumannii cell attachment. We first discovered that A. baumannii actively breaks down VA through the evolutionarily conserved vanABKP genes. These genes are under the control of the repressor VanR, which we show binds directly to VanR binding sites within the vanABKP genes bidirectional promoter. VA in turn counteracts VanR inhibition. We identified a VanR binding site and searched for it throughout the genome, especially in pili encoding promoter genes. We found a VanR binding site in the pilus encoding csu operon promoter and showed that VanR binds specifically to it. As expected, a strain lacking VanR overproduces Csu pili and makes robust biofilms. Our study uncovers the role that VA plays in facilitating the attachment of A. baumannii cells to surfaces, a crucial step in biofilm formation. These findings provide valuable insights into a previously obscure catabolic pathway with significant clinical implications.
Translation of the nine item avoidant/restrictive food intake disorder screen (NIAS) questionnaire in French (NIAS‐Fr)
Authors: Van Ouytsel Pauline , Mikhael‐Moussa Hiba , Mion François , Roman Sabine , Gourcerol Guillaume , Marion‐Letellier Rachel , Piessevaux Hubert , Louis Hubert , Melchior Chloé 
Published: February 2024

AbstractBackgroundThe Nine Item Avoidant/Restrictive Food Intake Disorder (ARFID) Screen (NIAS) questionnaire is originally available in English. Given the significant overlap of ARFID‐like symptoms in gastrointestinal (GI) diseases, ARFID screening becomes crucial in these patient populations. Consequently, the translation of the NIAS questionnaire into French is necessary for its utilization in French‐speaking countries.MethodsClinical experts in neuro‐gastroenterology and dietetics from four medical centres in two French‐speaking countries (France and Belgium) took part in a well‐structured questionnaire translation procedure. This process involved six steps before final approval: translation from English to French, backward translation, comparison between the original and retranslated versions, testing the translated version on patients, making corrections based on patient feedback, and testing the corrected version on an additional sample of patients.Key ResultsThe NIAS questionnaire in French (NIAS‐Fr) was tested on 18 outpatients across the involved centres. For the majority of native French‐speaking patients, the translated questionnaire was well understood and clear. After incorporating two relevant modifications suggested by the patients, the translated questionnaire was approved through testing on an additional sample of patients.Conclusions and InferencesThe involvement of two French‐speaking countries was crucial for the harmonization and cultural adaptation of the questionnaire. As a result, the NIAS‐Fr is now available for use in 54 French‐speaking countries, serving approximately 321 million French speakers across five continents for screening ARFID, for both clinical and research purposes.
Auxin co‐receptor IAA17/AXR3 controls cell elongation in Arabidopsis thaliana root solely by modulation of nuclear auxin pathway
Authors: Kubalová Monika , Müller Karel , Dobrev Petre Ivanov , Rizza Annalisa , Jones Alexander M. , Fendrych Matyáš 
Published: February 2024

Summary

The nuclear TIR1/AFB‐Aux/IAA auxin pathway plays a crucial role in regulating plant growth and development. Specifically, the IAA17/AXR3 protein participates in Arabidopsis thaliana root development, response to auxin and gravitropism. However, the mechanism by which AXR3 regulates cell elongation is not fully understood.

We combined genetical and cell biological tools with transcriptomics and determination of auxin levels and employed live cell imaging and image analysis to address how the auxin response pathways influence the dynamics of root growth.

We revealed that manipulations of the TIR1/AFB‐Aux/IAA pathway rapidly modulate root cell elongation. While inducible overexpression of the AXR3‐1 transcriptional inhibitor accelerated growth, overexpression of the dominant activator form of ARF5/MONOPTEROS inhibited growth. In parallel, AXR3‐1 expression caused loss of auxin sensitivity, leading to transcriptional reprogramming, phytohormone signaling imbalance and increased levels of auxin. Furthermore, we demonstrated that AXR3‐1 specifically perturbs nuclear auxin signaling, while the rapid auxin response remains functional.

Our results shed light on the interplay between the nuclear and cytoplasmic auxin pathways in roots, revealing their partial independence but also the dominant role of the nuclear auxin pathway during the gravitropic response of Arabidopsis thaliana roots.