bluuebunny/crossref_metadata_2025_split huggingface dataset:
A distinct Acyl-CoA binding protein (ACBP6) shapes tissue plasticity during nutrient adaptation in Drosophila
Authors: Li Xiaotong , Karpac Jason 
Published: November 2023

AbstractNutrient availability is a major selective force in the evolution of metazoa, and thus plasticity in tissue function and morphology is shaped by adaptive responses to nutrient changes. Utilizing Drosophila, we reveal that distinct calibration of acyl-CoA metabolism, mediated by Acbp6 (Acyl-CoA binding-protein 6), is critical for nutrient-dependent tissue plasticity. Drosophila Acbp6, which arose by evolutionary duplication and binds acyl-CoA to tune acetyl-CoA metabolism, is required for intestinal resizing after nutrient deprivation through activating intestinal stem cell proliferation from quiescence. Disruption of acyl-CoA metabolism by Acbp6 attenuation drives aberrant ‘switching’ of metabolic networks in intestinal enterocytes during nutrient adaptation, impairing acetyl-CoA metabolism and acetylation amid intestinal resizing. We also identified STAT92e, whose function is influenced by acetyl-CoA levels, as a key regulator of acyl-CoA and nutrient-dependent changes in stem cell activation. These findings define a regulatory mechanism, shaped by acyl-CoA metabolism, that adjusts proliferative homeostasis to coordinately regulate tissue plasticity during nutrient adaptation.
Identification of NAD-RNA species and ADPR-RNA decapping in Archaea
Authors: Gomes-Filho José Vicente , Breuer Ruth , Morales-Filloy Hector Gabriel , Pozhydaieva Nadiia , Borst Andreas , Paczia Nicole , Soppa Jörg , Höfer Katharina , Jäschke Andres , Randau Lennart 
Published: November 2023

AbstractNAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells.
Optomechanical ring resonator for efficient microwave-optical frequency conversion
Authors: Chen I-Tung , Li Bingzhao , Lee Seokhyeong , Chakravarthi Srivatsa , Fu Kai-Mei , Li Mo 
Published: November 2023

AbstractPhonons traveling in solid-state devices are emerging as a universal excitation for coupling different physical systems. Phonons at microwave frequencies have a similar wavelength to optical photons in solids, enabling optomechanical microwave-optical transduction of classical and quantum signals. It becomes conceivable to build optomechanical integrated circuits (OMIC) that guide both photons and phonons and interconnect photonic and phononic devices. Here, we demonstrate an OMIC including an optomechanical ring resonator (OMR), where co-resonant infrared photons and GHz phonons induce significantly enhanced interconversion. The platform is hybrid, using wide bandgap semiconductor gallium phosphide (GaP) for waveguiding and piezoelectric zinc oxide (ZnO) for phonon generation. The OMR features photonic and phononic quality factors of >1 × 105 and 3.2 × 103, respectively. The optomechanical interconversion between photonic modes achieved an internal conversion efficiency $${\eta }_{i}=(2.1\pm 0.1)\%$$

η

i

=

(

2.1
±
0.1

)

%
and a total device efficiency $${\eta }_{{tot}}=0.57{\times 10}^{-6}$$

η

t
o
t

=
0.57

×
10

−
6

at a low acoustic pump power of 1.6 mW. The efficient conversion in OMICs enables microwave-optical transduction for quantum information and microwave photonics applications.
Comprehensive energy efficiency optimization algorithm for steel load considering network reconstruction and demand response
Authors: Zang Yuxiu , Wang Shunjiang , Ge Weichun , Li Yaping , Cui Jia 
Published: November 2023

AbstractIndustrial loads are usually energy intensive and inefficient. The optimization of energy efficiency management in steel plants is still in the early stage of development. Considering the topology of power grid, it is an urgent problem to improve the operation economy and load side energy efficiency of steel plants. In this paper, a two-level collaborative optimization method is proposed, which takes into account the dynamic reconstruction cost, transmission loss cost, energy cost and demand response benefit. The upper level objective is the optimization of topology in the grid structure to optimize the power loss and dynamic reconstruction costs of the grid. The lower level is the energy cost considering demand response, real time price and dynamic demand response price. Firstly, the mathematical models of stable load, impact load and the steel production line load are built. The key parameters are identified by the Back Propagation neural network algorithm according to the actual production data. Secondly, considering the constraints of grid structure and load operation capacity, the impact of dynamic grid loss and real-time dynamic electricity price on the energy efficiency of the whole grid are analyzed in depth. The optimal operation model considering the dynamic reconfiguration and grid tramission loss of distribution network is built. Taking a steel plant park in Northeast China as an example, it is proved that the optimization model can improve energy efficiency on the load side by optimizing energy consumption and demand response participation time on load side. The energy cost is reduced by 17.77% on the load side, the network loss is reduced by 1.8%, and the operating cost of the power grid is reduced by 26.2%, which has a positive effect on improving energy utilization efficiency, reducing distribution network loss, and improving overall economic efficiency.
Protective effect of ligustrazine on oxidative stress and apoptosis following testicular torsion in rats
Authors: Chen Songmao , Liao Zhengjian , Zheng Tingting , Zhu Yuanfan , Ye Liefu 
Published: November 2023

AbstractTesticular torsion is a common urologic emergency and one of the causes of infertility in males. It has been reported that ligustrazine may decrease oxidative stress and reduce ischemia–reperfusion injury. This study aims to investigate the protective effect of ligustrazine in ischemia–reperfusion injury after testicular torsion-detorsion. First, 40 rats were randomly and equally divided into TMP (Ligustrazine) group, the Testicular torsion (T/D) group, the Sham (Sham operation) group, and Control group. The left testis of rats in the TMP and T/D group was rotated for 2 h. The TMP group was intraperitoneally injected with ligustrazine solution and the T/D and the Sham groups were injected with normal saline. The left testes of four groups were obtained for assay on the 4th day after the operation. Average level of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were higher in Sham and Control groups than T/D group and TMP group. Conversely, average level of malondialdehyde (MDA) and reactive oxygen species (ROS) was lower in Sham and Control groups than T/D group and TMP group. In contrast with the T/D group, SOD, GPX, and CAT enzymatic activities increased, whereas MDA and ROS content decreased in the TMP group (P < 0.05). Microscopic observation showed that the testicular tissue of the Sham and Control groups were basically normal. The TMP and T/D groups had significant testicular tissue damage, whereas the TMP group had less damage and apoptosis than the T/D group. The apoptotic index of germ cells in the TMP group (13.05 ± 4.41) was lower than the T/D group (30.23 ± 11.31) (P < 0.05) and higher (P < 0.05) than the Sham group (0.56 ± 0.29). So we found that Ligustrazine lowered ischemia–reperfusion injury after testicular torsion-detorsion by decreasing the reactive oxygen species and suppressing apoptosis.
Broad-spectrum metastasis suppressing compounds and therapeutic uses thereof in human tumors
Authors: Komlosh Pnina Gottfried , Chen Jonathan L. , Childs-Disney Jessica , Disney Matthew D. , Canaani Dan 
Published: November 2023

AbstractPreviously, we have identified a novel human metastasis-inducing lncRNA (named SKAI1BC), that suppresses the KAI1/CD82 metastasis-suppressing gene and is upregulated in triple negative breast cancer and melanoma derived cell lines. Modeling of the SKAI1BC lncRNA secondary structure and its potential interaction with Inforna compounds, led us to identify several compounds that might bind the SKAI1BC lncRNA. We found that these compounds inhibit metastasis invasion and cell migration in culture, in all eight types of solid human cancers tested: several of which are the most lethal and/or frequent human malignancies. Moreover, in most cases, the mechanism of action of several of our compounds involves enhancement of KAI1/CD82 RNA level depending on the specific compound and the human tumor type. With the epigenetic inactivation of KAI1/CD82 in at least ten additional solid human cancers, this implies a very good chance to broaden the spectrum of human cancers affected by our compounds. This is the first time that modeling of a large lncRNA (> 700 bp) secondary structure followed by its potential interaction with Inforna like compounds database has led to the identification of potential biologically active small molecule drugs.
Untargeted metabolomics reveal pathways associated with neuroprotective effect of oxyresveratrol in SH-SY5Y cells
Authors: Mahamud Nureesun , Songvut Phanit , Muangnoi Chawanphat , Rodsiri Ratchanee , Dahlan Winai , Tansawat Rossarin 
Published: November 2023

AbstractOxyresveratrol has been documented benefits for neurodegenerative disease. However, the specific molecular mechanisms and pathways involved is currently limited. This study aimed to investigate the potential neuroprotective mechanisms of oxyresveratrol using rotenone-induced human neuroblastoma SH-SY5Y cytotoxicity. Cells were divided into the following groups: control, rotenone, and oxyresveratrol pre-treated before being exposed to rotenone. Cellular assays were performed to investigate neuroprotective effects of oxyresveratrol. The results showed that 20 μM oxyresveratrol was effective in preventing rotenone-induced cell death and decreasing ROS levels in the cells. The alteration of metabolites and pathways involved in the neuroprotective activities of oxyresveratrol were further investigated using LC-QTOF-MS/MS untargeted metabolomics approach. We hypothesized that oxyresveratrol's neuroprotective effects would be associated with neurodegenerative pathways. A total of 294 metabolites were identified. 7,8-dihydrobiopterin exhibited the highest VIP scores (VIP > 3.0; p < 0.05), thus considered a biomarker in this study. Our results demonstrated that pretreatment with oxyresveratrol upregulated the level of 7,8-dihydrobiopterin compared to the positive control. Pathway analysis verified that 7,8-dihydrobiopterin was primarily associated with phenylalanine, tyrosine, and tryptophan metabolism (impact = 1, p < 0.001), serving as essential cofactors for enzymatic function in the dopamine biosynthesis pathway. In conclusion, oxyresveratrol may be benefit for the prevention of neurodegenerative diseases by increasing 7,8-dihydrobiopterin concentration.
Hepatocyte integrity depends on c-Jun-controlled proliferation in Schistosoma mansoni infected mice
Authors: Härle Lukas , von Bülow Verena , Knedla Lukas , Stettler Frederik , Müller Heike , Zahner Daniel , Haeberlein Simone , Windhorst Anita , Tschuschner Annette , Burg-Roderfeld Monika , Köhler Kernt , Grevelding Christoph G. , Roeb Elke , Roderfeld Martin 
Published: November 2023

AbstractSchistosomiasis is a parasitic disease affecting more than 250 million people worldwide. The transcription factor c-Jun, which is induced in S. mansoni infection-associated liver disease, can promote hepatocyte survival but can also trigger hepatocellular carcinogenesis. We aimed to analyze the hepatic role of c-Jun following S. mansoni infection. We adopted a hepatocyte-specific c-Jun knockout mouse model (Alb-Cre/c-Jun loxP) and analyzed liver tissue and serum samples by quantitative real-time PCR array, western blotting, immunohistochemistry, hydroxyproline quantification, and functional analyses. Hepatocyte-specific c-Jun knockout (c-JunΔli) was confirmed by immunohistochemistry and western blotting. Infection with S. mansoni induced elevated aminotransferase-serum levels in c-JunΔli mice. Of note, hepatic Cyclin D1 expression was induced in infected c-Junf/f control mice but to a lower extent in c-JunΔli mice. S. mansoni soluble egg antigen-induced proliferation in a human hepatoma cell line was diminished by inhibition of c-Jun signaling. Markers for apoptosis, oxidative stress, ER stress, inflammation, autophagy, DNA-damage, and fibrosis were not altered in S. mansoni infected c-JunΔli mice compared to infected c-Junf/f controls. Enhanced liver damage in c-JunΔli mice suggested a protective role of c-Jun. A reduced Cyclin D1 expression and reduced hepatic regeneration could be the reason. In addition, it seems likely that the trends in pathological changes in c-JunΔli mice cumulatively led to a loss of the protective potential being responsible for the increased hepatocyte damage and loss of regenerative ability.
Crystal plasticity simulations with representative volume element of as-build laser powder bed fusion materials
Authors: Bulgarevich Dmitry S. , Nomoto Sukeharu , Watanabe Makoto , Demura Masahiko 
Published: November 2023

AbstractAdditive manufacturing of as-build metal materials with laser powder bed fusion typically leads to the formations of various chemical phases and their corresponding microstructure types. Such microstructures have very complex shape and size anisotropic distributions due to the history of the laser heat gradients and scanning patterns. With higher complexity compared to the post-heat-treated materials, the synthetic volume reconstruction of as-build materials for accurate modelling of their mechanical properties is a serious challenge. Here, we present an example of complete workflow pipeline for such nontrivial task. It takes into account the statistical distributions of microstructures: object sizes for each phase, several shape parameters for each microstructure type, and their morphological and crystallographic orientations. In principle, each step in the pipeline, including the parameters in the crystal plasticity model, can be fine-tuned to achieve suitable correspondence between experimental and synthetic microstructures as well as between experimental stress–strain curves and simulated results. To our best knowledge, this work represents an example of the most challenging synthetic volume reconstruction for as-build additive manufacturing materials to date.
Functional genome analysis and anti-Helicobacter pylori activity of a novel bacteriocinogenic Lactococcus sp. NH2-7C from Thai fermented pork (Nham)
Authors: Kingkaew Engkarat , Woraprayote Weerapong , Booncharoen Auttaporn , Niwasabutra Kanidta , Janyaphisan Thitiphorn , Vilaichone Ratha-Korn , Yamaoka Yoshio , Visessanguan Wonnop , Tanasupawat Somboon 
Published: November 2023

AbstractHelicobacter pylori, linked to gastric diseases, is targeted for probiotic treatment through bacteriocin production. Bacteriocins have gained recognition for their non-toxic effects on host cells and their ability to combat a wide range of pathogens.This study aimed to taxonomically characterize and evaluate the safety and probiotic properties of the novel species ofLactococcussp. NH2-7C isolated from fermented pork, as well as its bacteriocin NH2-7C, both in vitro and in silico. Comparative genotypic analysis revealed an average nucleotide identity of 94.96%, an average amino acid identity of 94.29%, and a digital DNA-DNA hybridization value of 63.80% when compared toLactococcus lactissubsp.lactisJCM 5805T. These findings suggest that strain NH2-7C represents a novel species within the genusLactococcus. In silico assessments confirmed the non-pathogenic nature of strain NH2-7C and the absence of genes associated with virulence and biogenic amine formation. Whole-genome analysis revealed the presence of thenisAgene responsible for nisin A production, indicating its potential as a beneficial compound with anti-Helicobacter pyloriactivity and non-toxic characteristics. Probiotic assessments indicated bile salt hydrolase and cholesterol assimilation activities, along with the modulation of interleukin-6 and tumour necrosis factor-α secretion. Strain NH2-7C demonstrated gastrointestinal tolerance and the ability to adhere to Caco-2 cells, affirming its safety and probiotic potential. Additionally, its ability to produce bacteriocins supports its suitability as a functional probiotic strain with therapeutic potential. However, further in vitro and in vivo investigations are crucial to ensure its safety and explore potential applications forLactococcussp. NH2-7C as a probiotic agent.
Insight into the on/off switch that regulates expression of the MSMEG-3762/63 efflux pump in Mycobacterium smegmatis
Authors: Campolattano Nicoletta , D’Abrosca Gianluca , Russo Luigi , De Siena Barbara , Della Gala Milena , De Chiara Ida , Marasco Rosangela , Goff Aaron , Waddell Simon J. , Sacco Margherita , Muscariello Lidia 
Published: November 2023

AbstractDrug resistance is one of the most difficult challenges facing tuberculosis (TB) control. Drug efflux is among the mechanisms leading to drug resistance. In our previous studies, we partially characterized the ABC-type MSMEG-3762/63 efflux pump in Mycobacterium smegmatis, which shares high percentage of identity with the Mycobacterium tuberculosis Rv1687/86c pump. MSMEG-3762/63 was shown to have extrusion activity for rifampicin and ciprofloxacin, used in first and second-line anti-TB treatments. Moreover, we described the functional role of the TetR-like MSMEG-3765 protein as a repressor of the MSMEG_3762/63/65 operon and orthologous Rv1687/86/85c in M. tuberculosis. Here we show that the operon is upregulated in the macrophage environment, supporting a previous observation of induction triggered by acid-nitrosative stress. Expression of the efflux pump was also induced by sub-inhibitory concentrations of rifampicin or ciprofloxacin. Both these drugs also prevented the binding of the MSMEG-3765 TetR repressor protein to its operator in the MSMEG_3762/63/65 operon. The hypothesis that these two drugs might be responsible for the induction of the efflux pump operon was assessed by bioinformatics analyses. Docking studies using a structural model of the regulator MSMEG-3765 showed that both antibiotics abolished the ability of this transcriptional repressor to recognize the efflux pump operon by interacting with the homodimer at different binding sites within the same binding pocket. Reduced binding of the repressor leads to induction of the efflux pump in M. smegmatis, and reduced efficacy of these two anti-mycobacterial drugs.
Phosphodiesterase 4 inhibition after retrieval switches the memory fate favoring extinction instead of reconsolidation
Authors: Machado Batista Sohn Jeferson , Cardoso Nathalie Carla , Raymundi Ana Maria , Prickaerts Jos , Stern Cristina Aparecida Jark 
Published: November 2023

AbstractPhosphodiesterase 4 (PDE4), an enzyme expressed in the dorsal hippocampus (DH), hydrolyzes the cAMP, limiting the PKA-induced CREB phosphorylation (pCREB) and BDNF expression. Depending on the brain region, PKA and pCREB mediate reconsolidation or extinction, whereas BDNF is mainly related to extinction facilitation. The mechanisms underpinning the switch between reconsolidation and extinction are relatively unknown. Here, we tested the hypothesis that PDE4 might control these processes. We showed in Wistar rats submitted to contextual fear conditioning that PDE4 inhibition with roflumilast (ROF) within the DH, after a short retrieval, did not change freezing behavior after one day (TestA1). After 10 days, the ROF-treated group significantly reduced the expression of freezing behavior. This effect depended on retrieval, Test A1 exposure, and reinstated after a remainder foot shock, suggesting an extinction facilitation. The ROF effect depended on PKA after retrieval or, protein synthesis after Test A1. After retrieval, ROF treatment did not change the pCREB/CREB ratio in the DH. It enhanced proBDNF expression without changing pre-proBDNF or mature BDNF in the DH after Test A1. The results suggest that the inhibition of PDE4 in the DH after a short retrieval changes the memory sensibility from reconsolidation to extinction via regulating proBDNF expression.
Effect of automated head-thorax elevation during chest compressions on lung ventilation: a model study
Authors: Duhem Hélène , Terzi Nicolas , Segond Nicolas , Bellier Alexandre , Sanchez Caroline , Louis Bruno , Debaty Guillaume , Guérin Claude 
Published: November 2023

AbstractOur goal was to investigate the effects of head-thorax elevation (HUP) during chest compressions (CC) on lung ventilation. A prospective study was performed on seven human cadavers. Chest was automatically compressed-decompressed in flat position and during progressive HUP from 18 to 35°. Lung ventilation was measured with electrical impedance tomography. In each cadaver, 5 sequences were randomly performed: one without CC at positive end-expiratory pressure (PEEP) 0cmH2O, 3 s with CC at PEEP0, 5 or 10cmH2O and 1 with CC and an impedance threshold device at PEEP0cmH2O. The minimal-to-maximal change in impedance (VTEIT in arbitrary unit a.u.) and the minimal impedance in every breathing cycle (EELI) the) were compared between flat, 18°, and 35° in each sequence by a mixed-effects model. Values are expressed as median (1st–3rd quartiles). With CC, between flat, 18° and 35° VTEIT decreased at each level of PEEP. It was 12416a.u. (10,689; 14,442), 11,239 (7667; 13,292), and 6457 (4631; 9516), respectively, at PEEP0. The same was true with the impedance threshold device. EELI/VTEIT significantly decreased from − 0.30 (− 0.40; − 0.15) before to − 1.13 (− 1.70; − 0.61) after the CC (P = 0.009). With HUP lung ventilation decreased with CC as compared to flat position. CC are associated with decreased in EELI.
Investigating the efficiency of oak powder as a new natural coagulant for eliminating polystyrene microplastics from aqueous solutions
Authors: Esmaeili Nasrabadi Afsaneh , Zahmatkesh Anbarani Mohaddeseh , Bonyadi Ziaeddin 
Published: November 2023

AbstractPolystyrene (PS) is a commonly used plastic material in disposable containers. However, it readily breaks down into microplastic particles when exposed to water environments. In this research, oak powder was used as a natural, inexpensive, and eco-friendly coagulant. The present study aims to determine the effectiveness of oak powder in removing PS from aquatic environments. The Box-Behnken model (BBD) was used to determine the optimal conditions for removal. The removal efficiency was evaluated for various parameters including PS concentration (100–900 mg/L), pH (4–10), contact time (10–40 min), and oak dosage (100–400 mg/L). The maximum removal of PS microplastics (89.1%) was achieved by using an oak dose of 250 mg/L, a PS concentration of 900 mg/L, a contact time of 40 min, and a pH of 7. These results suggest that oak powder can effectively remove PS microplastics through surface adsorption and charge neutralization mechanisms, likely due to the presence of tannin compounds. Based on the results obtained, it has been found that the natural coagulant derived from oak has the potential to effectively compete with harmful chemical coagulants in removing microplastics from aqueous solutions.