bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct features of B cell receptors in neuromyelitis optica spectrum disorder among CNS inflammatory demyelinating diseases
Authors: Kim Hyo Jae , Park Jong-Eun , Shin Wangyong , Seo Dayoung , Kim Seungmi , Kim Hyunji , Noh Jinsung , Lee Yonghee , Kim Hyunjin , Lim Young-Min , Kim Hyori , Lee Eun-Jae 
Published: October 2023

AbstractBackgroundNeuromyelitis optica spectrum disorder (NMOSD) stands out among CNS inflammatory demyelinating diseases (CIDDs) due to its unique disease characteristics, including severe clinical attacks with extensive lesions and its association with systemic autoimmune diseases. We aimed to investigate whether characteristics of B cell receptors (BCRs) differ between NMOSD and other CIDDs using high-throughput sequencing.MethodsFrom a prospective cohort, we recruited patients with CIDDs and categorized them based on the presence and type of autoantibodies: NMOSD with anti-aquaporin-4 antibodies, myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) with anti-myelin oligodendrocyte glycoprotein antibodies, double-seronegative demyelinating disease (DSN), and healthy controls (HCs). The BCR features, including isotype class, clonality, somatic hypermutation (SHM), and the third complementarity-determining region (CDR3) length, were analyzed and compared among the different disease groups.ResultsBlood samples from 33 patients with CIDDs (13 NMOSD, 12 MOGAD, and 8 DSN) and 34 HCs were investigated for BCR sequencing. Patients with NMOSD tended to have more activated BCR features compare to the other disease groups. They showed a lower proportion of unswitched isotypes (IgM and IgD) and a higher proportion of switched isotypes (IgG), increased clonality of BCRs, higher rates of SHM, and shorter lengths of CDR3. Notably, advanced age was identified as a clinical factor associated with these activated BCR features, including increased levels of clonality and SHM rates in the NMOSD group. Conversely, no such clinical factors were found to be associated with activated BCR features in the other CIDD groups.ConclusionsNMOSD patients, among those with CIDDs, displayed the most pronounced B cell activation, characterized by higher levels of isotype class switching, clonality, SHM rates, and shorter CDR3 lengths. These findings suggest that B cell-mediated humoral immune responses and characteristics in NMOSD patients are distinct from those observed in the other CIDDs, including MOGAD. Age was identified as a clinical factor associated with BCR activation specifically in NMOSD, implying the significance of persistent B cell activation attributed to anti-aquaporin-4 antibodies, even in the absence of clinical relapses throughout an individual’s lifetime.
Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome
Authors: Angerfors Annelie , Brännmark Cecilia , Lagging Cecilia , Tai Kara , Månsby Svedberg Robert , Andersson Björn , Jern Christina , Stanne Tara M. 
Published: October 2023

AbstractBackgroundThe inflammatory response to cerebral ischemia is complex; however, most clinical studies of stroke outcome focus on a few selected proteins. We, therefore, aimed to profile a broad range of inflammation-related proteins to: identify proteins associated with ischemic stroke outcome that are independent of established clinical predictors; identify proteins subsets for outcome prediction; and perform sex and etiological subtype stratified analyses.MethodsAcute-phase plasma levels of 65 inflammation-related proteins were measured in 534 ischemic stroke cases. Logistic regression was used to estimate associations to unfavorable 3-month functional outcome (modified Rankin Scale score > 2) and LASSO regressions to identify proteins with independent effects.ResultsTwenty proteins were associated with outcome in univariable models after correction for multiple testing (FDR < 0.05), and for 5 the association was independent of clinical variables, including stroke severity (TNFSF14 [LIGHT], OSM, SIRT2, STAMBP, and 4E-BP1). LASSO identified 9 proteins that could best separate favorable and unfavorable outcome with a predicted diagnostic accuracy (AUC) of 0.81; three associated with favorable (CCL25, TRAIL [TNFSF10], and Flt3L) and 6 with unfavorable outcome (CSF-1, EN-RAGE [S100A12], HGF, IL-6, OSM, and TNFSF14). Finally, we identified sex- and etiologic subtype-specific associations with the best discriminative ability achieved for cardioembolic, followed by cryptogenic stroke.ConclusionsWe identified candidate blood-based protein biomarkers for post-stroke functional outcome involved in, e.g., NLRP3 inflammasome regulation and signaling pathways, such as TNF, JAK/STAT, MAPK, and NF-κB. These proteins warrant further study for stroke outcome prediction as well as investigations into the putative causal role for stroke outcome.
Laboratory indices in patients with osteonecrosis of the femoral head: a retrospective comparative study
Authors: Zheng Haotian , Ye Binglin , Huang Kesong , Gao Xiangming , Chen Wei 
Published: October 2023

Abstract
Background
Osteonecrosis of the femoral head is a degenerative condition linked to corticosteroids, alcoholism, or trauma. With its rising prevalence due to increased hormone drug use and its debilitating effects on young to middle-aged individuals, understanding its association with specific laboratory indicators can aid early diagnosis and prevention.

Methods
Upon retrospective analysis of the clinical data pertaining to individuals diagnosed with femoral head necrosis, spanning from January 2016 to January 2022, a comprehensive evaluation was conducted within the same time frame. The study aimed to ascertain the presence of femoral head necrosis in a total of 1176 individuals. A total of 1036 healthy patients were recruited randomly, ensuring that their ages matched. The risk variables associated with the utilization of logistic regression analysis and analysis techniques are employed. The patient examines the age distribution within a specific age group.

Results
The levels of high-density lipoprotein, low-density lipoprotein A1, lipoprotein B1, total protein, albumin, globulin, and other lipophilic metabolism and coagulation markers exhibited a statistically significant increase compared to the control group. A multifactor logistic regression analysis was conducted to identify potential risk factors associated with femoral head necrosis in patients.

Conclusion
Femoral head necrosis is associated with a range of variables including coagulation malfunction, lipid metabolic abnormalities, and inflammation.
Impaired immunosuppressive effect of bone marrow mesenchymal stem cell-derived exosomes on T cells in aplastic anemia
Authors: Wang Shichong , Huo Jiali , Liu Yilin , Chen Lingyun , Ren Xiang , Li Xingxin , Wang Min , Jin Peng , Huang Jinbo , Nie Neng , Zhang Jing , Shao Yingqi , Ge Meili , Zheng Yizhou 
Published: October 2023

Abstract
Background
Previous studies have verified the dysfunction of mesenchymal stem cells (MSCs) for immunoregulation in acquired aplastic anemia (AA) patients. Exosomes derived from MSCs can partially substitute MSCs acting as immune regulator. Dysfunction of exosomes (Exos) derived from AA-MSC (AA-Exos) may play a key role in immunologic dissonance.

Method
In this study, CD3 + T cells were collected and cocultured with AA-Exos and exosomes derived from HD-MSC (HD-Exos). The proliferation, differentiation and activation of CD3 + T cells were detected to compare the immunosuppressive effects between AA-Exos and HD-Exos. An immune-mediated murine model of AA was structured to compare the therapeutic effect of AA-Exos and HD-Exos. Furthermore, total RNA including miRNA from exosomes we purified and total RNA of CD3 + T cells were extracted for RNA-seq in order to construct the miRNA–mRNA network for interactions and functional analysis.

Results
AA-Exos had impaired inhibition effects on CD3 + T cells in terms of cell proliferation, activation and differentiation compared with exosomes from HD-Exos. HD-Exos showed a more effective rescue of AA mice compared to AA-Exos. Importantly, we found some differentially expressed miRNA involved in immune response, such as miR-199, miR-128 and miR-486. The Gene Ontology analysis of differentially expressed genes (DEGs) revealed involvement of various cellular processes, such as lymphocyte chemotaxis, lymphocyte migration and response to interferon-gamma. The Kyoto Encyclopedia of Genes and Genomes analysis illustrated upregulation of critical pathways associated with T cell function after coculturing with AA-Exos compared with HD-Exos, such as graft-versus-host disease, Th17 cell differentiation and JAK-STAT signaling pathway. A miRNA–mRNA network was established to visualize the interaction between them.

Conclusion
In summary, AA-Exos had impaired immunosuppressive effect on T cells, less ability to rescue AA mice and differently expressed miRNA profile, which might partly account for the pathogenesis of AA as well as provide a new target of AA treatment.
Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie
Authors: Betancor Marina , Marín Belén , Otero Alicia , Hedman Carlos , Romero Antonio , Barrio Tomás , Sevilla Eloisa , Douet Jean-Yves , Huor Alvina , Badiola Juan José , Andréoletti Olivier , Bolea Rosa 
Published: October 2023

AbstractThe emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle.
Current status and further development of deterministic lateral displacement for micro-particle separation
Authors: Zhbanov Alexander , Lee Ye Sung , Yang Sung 
Published: October 2023

AbstractDeterministic lateral displacement (DLD) is a passive, label-free, continuous-flow method for particle separation. Since its discovery in 2004, it has been widely used in medical tests to separate blood cells, bacteria, extracellular vesicles, DNA, and more. Despite the very simple idea of the DLD method, many details of its mechanism are not yet fully understood and studied. Known analytical equations for the critical diameter of separated particles include only the gap between the columns in the DLD array and the fraction of the column shift. The dependence of the critical diameter on the post diameter, channel height, and a number of other geometric parameters remains unexplored. The problems also include the effect of flow rate and particle concentration on the critical diameter and separation efficiency. At present, DLD devices are mainly developed through numerical simulation and experimental validation. However, it is necessary to find fundamental regularities that would help to improve the separation quantitatively and qualitatively. This review discusses the principle of particle separation, the physical aspects of flow formation, and hydrodynamic forces acting on particles in DLD microchannels. Various analytical models of a viscous flow in an array of cylindrical posts are described. Prospects for further research are outlined.
The impact of high-immersion virtual reality on foreign language anxiety
Authors: Kaplan-Rakowski Regina , Gruber Alice 
Published: October 2023

AbstractPublic speaking, especially in a foreign language, is associated with increased anxiety. Research has shown the potential of virtual reality (VR) for simulating real-life experiences, allowing for public speaking practice in an ecological and safe environment. This between-subjects study investigated the effect of VR on foreign language anxiety (FLA) in public speaking practice. Intermediate learners of English participated in eight public speaking sessions over a three-month period, yielding 160 research observations. The experimental intervention took place in high-immersion VR with subjects wearing a VR headset and speaking in front of virtual audience. In the control intervention, subjects used a videoconferencing platform (Zoom) to speak in front of a real-life audience. Multivariate regression analysis revealed that practicing speaking in VR was associated with statistically significant lower FLA scores, compared with speaking practice using Zoom. The study found that VR technology had a positive effect on practicing public speaking in a foreign language. The research findings have practical implications for professionals and curriculum designers in various domains where public speaking skills are essential. For example, incorporating VR-based public speaking practice can benefit professionals preparing for a job interview, an elevator pitch, or a conference presentation. Curriculum designers can consider integrating VR simulations into language courses to provide students with realistic public speaking experiences. This approach can help students overcome language barriers, reduce anxiety, and develop their communication skills in a controlled and supportive environment.
Ethnic cuisine ratings of consumers: a sample analysis of Konya ethnic Mevlevi kitchen dishes’
Authors: Sormaz Ümit , Dursun Kadir , Onur Neslihan 
Published: October 2023

AbstractMevlevi cuisine, which carries the traces of history in Konya in Turkey, is an ethnic type of cuisine that is identified with that region. It is known that with the opening of more restaurants in the concept of ethnic cuisine, there will be a positive interaction in the region in terms of gastronomic tourism. Purpose of the research: To evaluate the meals and drinks of ethnic cuisine by the guests who prefer ethnic restaurants, using the menu analysis method. Case study, one of the qualitative research models, was used in the research. The research sample consists of Somatçı Fihi Ma Fih restaurant, which tries to keep an ethnic culinary culture alive with the concept of Mevlevi cuisine in Turkey’s Konya province and volunteered to participate in the research. The data collection phase of the research was carried out in the light of the interview, document review and information obtained with both the manager and the chef of this restaurant, which constitutes the sample of the research. As a result of the study, a total of 14 menu items selected from the menu were subjected to menu engineering analysis. After this analysis, a total of 6 products were included in the stars group, 4 products in the problem group, 3 products in the horses group and 1 product in the puzzles group. As a result of the research, it was suggested that the Mevlevi cuisine dishes, which are not liked by the guests who prefer ethnic dishes, should be removed from the menu and replaced with the ones with stronger taste.
Assessment of cognitive function in children with temporal lobe epilepsy using mismatch negativity
Authors: Talaat Hossam Sanyelbhaa , Moaty Asmaa Salah , Naby Sameh A. Abd El , Elgammal Hadeer M. , Zein-Elabedein Ahmed Mahmoud 
Published: October 2023

Abstract
Objectives
This study aimed to assess cognitive function in children with temporal lobe epilepsy (TLE) using mismatch negativity (MMN) potentials.

Background
Temporal lobe epilepsy (TLE) is a neurological condition that causes cognitive and behavioral problems. These complications were found to be affected by many factors including underlying neuropathology, age of onset, psychological issues, and therapeutic side effects that exacerbate pathophysiology of the condition.

Subject and method
In this case–control study, 44 children were involved and were divided into two groups: the study group involved 22 children with TLE, while the control group involved 22 normal children. Pure-tone audiometry, immittancemetry, and measurement of mismatch negativity (MMN) test were done for all subjects in this study.

Results
Latency of MMN was significantly increased, while the amplitude was significantly decreased in the study group compared to the control group. Abnormal MMN test was obtained in TLE patients.

Conclusion
Prolonged MMN latencies and decreased amplitudes in patients indicate impaired cognitive function in children with TLE compared with controls.
MyPathway Human Epidermal Growth Factor Receptor 2 Basket Study: Pertuzumab + Trastuzumab Treatment of a Tissue-Agnostic Cohort of Patients With Human Epidermal Growth Factor Receptor 2–Altered Advanced Solid Tumors
Authors: Sweeney Christopher J. , Hainsworth John D. , Bose Ron , Burris Howard A. , Kurzrock Razelle , Swanton Charles , Friedman Claire F. , Spigel David R. , Szado Tania , Schulze Katja , Price Richard , Malato Julia , Lo Amy A. , Levy Jonathan , Wang Yong , Yu Wei , Meric-Bernstam Funda 
Published: October 2023

Clinical trials frequently include multiple end points that mature at different times. The initial report, typically based on the primary end point, may be published when key planned co-primary or secondary analyses are not yet available. Clinical Trial Updates provide an opportunity to disseminate additional results from studies, published in JCO or elsewhere, for which the primary end point has already been reported. The MyPathway multiple-basket study (ClinicalTrials.gov identifier: NCT02091141 ) is evaluating targeted therapies in nonindicated tumors with relevant molecular alterations. We assessed pertuzumab + trastuzumab in a tissue-agnostic cohort of adult patients with human epidermal growth factor receptor 2 (HER2)–amplified and/or –overexpressed and/or –mutated solid tumors. The primary end point was objective response rate (ORR); secondary end points included survival and safety. At data cutoff (March 2022), 346 patients with HER2 amplification and/or overexpression with/without HER2 mutations (n = 263), or HER2 mutations alone (n = 83) had been treated. Patients with HER2 amplification and/or overexpression had an ORR of 25.9% (68/263, 95% CI, 20.7 to 31.6), including five complete responses (urothelial [n = 2], salivary gland [n = 2], and colon [n = 1] cancers). Activity was higher in those with wild-type (ORR, 28.1%) versus mutated KRAS (ORR, 7.1%). Among patients with HER2 amplification, ORR was numerically higher in patients with immunohistochemistry (IHC) 3+ (41.0%; 32/78) or 2+ (21.9%; 7/32), versus 1+ (8.3%; 1/12) or no expression (0%; 0/20). In patients with HER2 mutations alone, ORR was 6.0% (5/83, 95% CI, 2.0 to 13.5). Pertuzumab + trastuzumab showed activity in various HER2-amplified and/or -overexpressed tumors with wild-type KRAS, with the range of activity dependent on tumor type, but had limited activity in the context of KRAS mutations, HER2 mutations alone, or 0-1+ HER2 expression.
Fasting Modulates GABAergic Synaptic Transmission to Arcuate Kisspeptin Neurons in Female Mice
Authors: Mansano Naira da Silva , Vieira Henrique Rodrigues , Araujo-Lopes Roberta , Szawka Raphael Escorsim , Donato Jose , Frazao Renata 
Published: October 2023

Abstract
It is well-established that the hypothalamic–pituitary–gonadal (HPG) axis is suppressed due to negative energy balance. However, less information is available on whether kisspeptin neuronal activity contributes to fasting-induced responses. In the present study, female and male mice were fasted for 24 hours or provided food ad libitum (fed group) to determine whether acute fasting is sufficient to modulate kisspeptin neuronal activity. In female mice, fasting attenuated luteinizing hormone (LH) and prolactin (PRL) serum levels and increased follicle-stimulating hormone levels compared with the fed group. In contrast, fasting did not affect gonadotropin or PRL secretion in male mice. By measuring genes related to LH pulse generation in micropunches obtained from the arcuate nucleus of the hypothalamus (ARH), we observed that fasting reduced Kiss1 mRNA levels in female and male mice. In contrast, Pdyn expression was upregulated only in fasted female mice, whereas no changes in the Tac2 mRNA levels were observed in both sexes. Interestingly, the frequency and amplitude of the GABAergic postsynaptic currents recorded from ARH kisspeptin neurons (ARHKisspeptin) were reduced in 24-hour fasted female mice but not in males. Additionally, neuropeptide Y induced a hyperpolarization in the resting membrane potential of ARHKisspeptin neurons of fed female mice but not in males. Thus, the response of ARHKisspeptin neurons to fasting is sexually dependent with a female bias, associated with changes in gonadotropins and PRL secretion. Our findings suggest that GABAergic transmission to ARHKisspeptin neurons modulates the activity of the HPG axis during situations of negative energy balance.
Azulene-Fused Linearly π-Extended Polycyclic Aromatic Compounds: Synthesis, Photophysical Properties, and OFETs Applications
Authors: Murai Masahito , Iba Shinji , Hamao Shino , Kubozono Yoshihiro , Ota Hiromi , Takai Kazuhiko 
Published: October 2023

Abstract
By introducing azulene into linearly π-extended polycyclic aromatic hydrocarbons, we found that the HOMO–LUMO gap can be narrowed comparable to [n]acenes while affording high stability against air under visible light. The fabricated OFET devices with one of the derivatives exihibited typical p-type behavior under ambient conditions.
Wrapping of Drug Assembly with Silica Layer and Confinement through Reversible Linkage with Copper(II) for Glutathione-responsive Nanocapsule
Authors: Matsune Hideki , Yoshida Ryoya , Ikemizu Ren , Shiomori Koichiro , Yamamoto Tsuyoshi , Kishida Masahiro 
Published: October 2023

Abstract
Colloidal nano-assemblies containing doxorubicin (DOX) are prepared for the first time by co-precipitation with gluconate. The spherical assembly is wrapped with a porous silica layer via sol-gel process, and confined in the formed cage by treatment with copper(II) ions (Cu2+). The reversible linking of DOX with Cu2+ is allowed to suppress the leakage of DOX, but quick release is turned on in response to glutathione (GSH). The synthesized stimulus-responsive nanocapsules are characterized, and examined in a release test with/without GSH and applied to living HeLa cells.