bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaccination with mRNA-encoded membrane-bound HIV Envelope trimer induces neutralizing antibodies in animal models
Authors: Ramezani-Rad Parham , Cottrell Christopher A. , Marina-Zárate Ester , Liguori Alessia , Landais Elise , Torres Jonathan L. , Myers Amber , Lee Jeong Hyun , Baboo Sabyasachi , Flynn Claudia , McKenney Katherine , Salcedo Eugenia , Zhou Xiaoya , Kalyuzhniy Oleksandr , Georgeson Erik , Phelps Nicole , Lu Danny , Eskandarzadeh Saman , Menis Sergey , Kubitz Michael , Groschel Bettina , Alavi Nushin , Jackson Abigail M. , Lee Wen-Hsin , Tran Andy S. , Ben-Akiva Elana , Michaels Katarzyna Kaczmarek , Diedrich Jolene K. , Enemuo Chiamaka A. , Lewis Vanessa , Pradhan Arpan , Kasturi Sudhir Pai , Schiffner Torben , Steichen Jon M. , Carnathan Diane G. , Himansu Sunny , Yates John R. , Paulson James C. , Ozorowski Gabriel , Irvine Darrell J. , Silvestri Guido , Sok Devin , Ward Andrew B. , Crotty Shane , Schief William R. 
Published: January 2025

AbstractA protective vaccine against HIV will likely need to induce broadly neutralizing antibodies (bnAbs) that engage relatively conserved epitopes on the HIV envelope glycoprotein (Env) trimer. Nearly all vaccine strategies to induce bnAbs require the use of relatively complex immunization regimens involving a series of different immunogens, most of which are Env trimers. Producing protein-based clinical material to evaluate such relatively complex regimens in humans presents major challenges in cost and time. Furthermore, immunization with HIV trimers as soluble proteins induces strong non-neutralizing responses to the trimer base, which is normally occluded on the virion. These base responses could potentially detract from the induction of nAbs and the eventual induction of bnAbs. mRNA vaccine platforms offer potential advantages over protein delivery for HIV vaccine development, including increased production speed, reduced cost, and the ability to deliver membrane-bound trimers that might facilitate improved immuno-focusing to non-base epitopes. We report the design of mRNA-delivered soluble and membrane-bound forms of a stabilized native-like Env trimer (BG505 MD39.3), initial immunogenicity evaluation in rabbits that triggered clinical evaluation, and more comprehensive evaluation of B cell, T cell, and antibody responses in non-human primates. mRNA-encoded membrane-bound Env immunization elicited reduced off-target base-directed Env responses and stronger neutralizing antibody responses, compared with mRNA-encoded soluble Env. Overall, mRNA delivery of membrane-bound Env appears promising for enhancing B cell responses to subdominant epitopes and facilitating rapid translation to clinical testing, which should assist HIV vaccine development.One Sentence SummaryHIV envelope trimer mRNA enables membrane-bound expression and represents a functional immunogen in pre-clinical mammalian models.
Plastoquinone redox status influences carboxysome integrity via a RpaA- and ROS-dependent regulatory network
Authors: Santos-Merino María , Nikkanen Lauri , Kokarakis Emmanuel J. , Allahverdiyeva Yagut , Ducat Daniel C. 
Published: January 2025

AbstractCarboxysomes are bacterial microcompartments that encapsulate Rubisco and are a core component of the cyanobacterial carbon concentration mechanism (CCM). While carboxysome number, size and spatial organization are observed to vary in different environmental conditions (CO2, light, temperature, light quality), molecular mechanisms underlying this potentially adaptive process remain elusive. Herein, we observed that mutants of the circadian rhythm/metabolism factor, Regulator of Phycobilisome Associated A (RpaA), exhibit a striking breakdown of carboxysomes under certain environmental conditions. We find that growth conditions leading to overreduction of the plastoquinone (PQ) pool (mixotrophic growth, high irradiance, or chemical inhibition of electron transfer from PQ to the cytochromeb6fcomplex) are accompanied by elevated generation of reactive oxygen species (ROS), and correlate with carboxysome breakdown. Carboxysome breakdown is reversed by environmental conditions or chemical inhibitors that prevent PQ overreduction and accompanying ROS generation. Taken together, our data supports a novel link between the redox status of the PQ pool and carboxysome status and/or integrity. Our results have implications for fundamental understanding of cyanobacterial energy balancing pathways and may indicate new research directions for understanding how the carboxysome is remodeled in response to changing environments.
The role of entrepreneurial alertness and temporal focus in fostering sustainable entrepreneurship in young family firms
Authors: Futre Bruno , Crespo Nuno Fernandes 
Published: January 2025

PurposeThis study examines the relationship between entrepreneurial alertness, long-term and short-term focus and sustainable entrepreneurship in the context of young family firms. Additionally, we investigate how family management and long-term/short-term focus moderate the relationship between entrepreneurial alertness and sustainable entrepreneurship. Our findings contribute to a deeper understanding of the factors influencing sustainable entrepreneurship in family firms.Design/methodology/approachThis quantitative study investigated 707 young family firms in Portugal. Structural equation modeling (SEM) was employed to analyze the data and test the proposed hypotheses.FindingsThe results indicate a positive relationship between young family firms’ entrepreneurial alertness and sustainable entrepreneurship. Also relevant is that long-term focus is positively related to sustainable entrepreneurship, while short-term focus shows a negative relationship, challenging the temporal ambidexterity of new ventures. Conversely, two moderating effects were found: family management weakens and short-term focus strengthens the relationship between entrepreneurial alertness and sustainable entrepreneurship.Originality/valueAs far as we know, this study is the first one to explore the link between the entrepreneurial alertness of young family firms and sustainable entrepreneurship. It is also the first study to explore the contradictory impacts of both long-term focus and short-term focus on sustainable entrepreneurship.
Bridging role conflict and active lurking: the role of transformational leadership in B2B online communities
Authors: Nguyen Mai , Septianto Felix , Das Gopal , Malik Ashish 
Published: January 2025

Purpose
In the turbulence of a business-to-business (B2B) environment, employees often face role conflict as the result of changes in task allocations due to work requirements. This research uses organizational role theory to investigate the impact of role conflict on active lurking in B2B online communities. Active lurking, defined as the behavior of seeking information and acquiring knowledge without contributing, contrasts with posters who actively share knowledge. By focusing on these dynamics, this study aims to deepen the understanding of knowledge management (KM) practices and behaviors within B2B contexts.

Design/methodology/approach
This research conducts two experimental studies with a behavioral outcome to examine the impact of role conflict on active lurking and the mediating role of knowledge self-efficacy in this regard.

Findings
The results also indicate the moderating role of transformational leadership, such that when transformational leadership is low, role conflict decreases knowledge self-efficacy and active lurking. In contrast, this effect is attenuated in a high transformational leadership environment. The findings contribute to the KM literature by demonstrating how role conflict influences knowledge-sharing behaviors in B2B contexts and by capturing active lurking using a behavioral measure.

Practical implications
The implications of this study offer strategies to address role conflict and mitigate its negative impact. By integrating the concepts of posters and lurkers into the research framework, this paper offers a fresh perspective on organizational role conflict and KM. It provides insights into lurkers and active lurking behavior in B2B online communities, thereby extending the KM literature.

Originality/value
The findings of this research are novel as they first show the moderating role of transformational leadership, wherein when transformational leadership is low, role conflict decreases knowledge self-efficacy and active lurking and second it extends the literature on online knowledge sharing in a B2B context by capturing the role of active lurking through a behavioral measure.
Unlocking the power of AI in education: students’ intentions and AI tool use driving learning success in an emerging economy
Authors: Saha Priya , Hossain Md. Shakhawat , Roy Nirmal Chandra , Masud Abdullah Al , Amin Ruhul 
Published: January 2025

Purpose
This study aims to evaluate students’ intention and actual use (AU) of artificial intelligence (AI) tools’ to discover how the power of AI influences learning and academic success.

Design/methodology/approach
This paper used the unified theory of acceptance and use of technology (UTAUT) to develop a structural equation model (SEM) and used convenience sampling to measure 304 students’ five-point Likert scale responses. The model was tested with AMOS-24 and SPSS-25, and the study found that AI boosted students’ learning experiences and explain importance of AI skills and knowledge.

Findings
Performance expectancy (PE), effort expectancy (EE), social influence and facilitating condition directly and indirectly affect AU via intent to use (IU), while subjective norms determining the use of AI tools’ and have no substantial influence. Attitude (ATT) moderates PE and EE, although the data show that ATT has no substantial effect on EE.

Originality/value
These insights may help student to understand how AI tools’ benefit them and what factors affect their utilization. When correctly designed and executed, UTAUT provides an appropriate integrated theoretical framework for robust statistical analysis like SEM.
A novel heuristic programming-based intelligent controller for autonomous farming
Authors: Mukherjee Subhradip 
Published: January 2025

Purpose
This paper aims to develop one heuristic programming approach-based intelligent controller with unique necessary conditions for autonomous farming in a crop field, which improves path navigation and fewer damages to the vegetable crops in given environments. The proposed controller has also used the object detection and pesticide spraying technique with a ground robotic vehicle (GRv).

Design/methodology/approach
The controller contains a unique cost function, which approximates the cost of the easiest solution for the entire operation. Every time the main loop of the controller iterates, it detects the best-estimated solution from the given values. One path planning strategy is implemented in a crop field with the GRv in the simulated and experimental environment. The GRv moves to different planned positions of the crop field by avoiding obstacles and also optimizes the travelled path.

Findings
The proposed controller is also compared with the existing approaches to validate its efficacy. In comparison to the modified DAYANI and the advanced sine-cosine algorithm-advanced ant colony optimization strategy, the heuristic programming approach-based intelligent controller has convincingly improved the length of the navigational path by 4.53% and 3.7%, respectively.

Originality/value
In real time, the highest speed achieved by the GRv in the crop field is approximately 1.5 km/h. The proposed controller performs successfully in the given environments.
Deep reinforcement learning-based variable impedance control for grinding workpieces with complex geometry
Authors: Li Yanghong , Wang Yahao , Li Zhen , Yingxiang Lv , Chai Jin , Dong Erbao 
Published: January 2025

Purpose
This paper aims to design a deep reinforcement learning (DRL)-based variable impedance control policy that supports stability analysis for robot force tracking in complex geometric environments.

Design/methodology/approach
The DRL-based variable impedance controller explores and pre-learns the optimal policy for impedance parameter tuning in simulation scenarios with randomly generated workpieces. The trained results are then used as feedforward inputs to improve the force-tracking performance of the robot during contact. Based on Lyapunov’s theory, the stability of the proposed control policy is analysed to illustrate the interpretability of the results.

Findings
Simulations and experiments are performed on different types of complex environments. The results show that the proposed method is not only theoretically feasible but also has better force-tracking effects in practice.

Originality/value
Compared with most other DRL-based control policies, the proposed method possesses stability and interpretability, effectively avoids the overfitting phenomenon and thus has better simulation-to-real deployment results.
An Industrial Policy for Hairdressing?
Authors: Tomlinson Jim 
Published: January 2025

AbstractIndustrial policy is undergoing a notable revival in Britain, as well as elsewhere. But what are the aims of such a policy? The current government's core statement wholly subordinates its industrial strategy to the pursuit of economic growth. Even leaving aside the questions about the wisdom of making this the overarching goal of economic policy, there are issues about how far industrial policy should be focussed on other objectives. If one sees ‘good jobs’ as such an objective, how far can industrial policy be used in its pursuit? This article takes hairdressing as a perhaps counter‐intuitive example of where industrial policy might usefully be applied.
Effects of sugars on the gustatory response, longevity and realized fecundity of the egg parasitoid Trissolcus basalis
Authors: Geerinck Margot W. J. , Stockmans Isabelle , Wäckers Felix , Cusumano Antonino , Jacquemyn Hans , Lievens Bart 
Published: January 2025

AbstractParasitoids are widely used as biological control agents to manage insect pest populations in greenhouses. To meet their energetic and nutritional requirements, adult parasitoids consume carbohydrate‐rich sources such as (extra‐)floral nectar and honeydew. However, these sugar sources are often scarce or even absent in greenhouses, compromising the effectiveness of parasitoids as biological control agents. To remedy this, artificial sugar solutions can be provided to sustain parasitoid populations. To develop an artificial sugar solution that supports parasitoids, proper selection of sugars is critical. In this study, we investigated the innate gustatory response and survival of the egg parasitoid Trissolcus basalis (Wollaston) on eight plant‐ and/or insect‐derived sugars (fructose, glucose, maltose, mannose, melezitose, rhamnose, sucrose and trehalose). Our results show that T. basalis can consume a wide range of sugars and survive on them. Five sugars (sucrose, fructose, maltose, melezitose and trehalose) increased its longevity more than seven‐fold compared to the water control. For two sugars, sucrose and trehalose, the parasitoid's realized fecundity was monitored after various time periods during which the parasitoids were fed with the sugars (1 day, 1 week, 2 weeks, and 3 weeks). The results indicate a prolonged period of parasitism compared to the water control because of a longer lifespan when fed on the sugars. Altogether, our findings provide valuable insights for the development of an artificial sugar solution that supports the performance of T. basalis, potentially enhancing the biological control of important pest species such as Nezara viridula (Linnaeus).
Short (2‐Hour) Non‐Oxygenated End‐Ischemic Hypothermic Perfusion Versus Cold Storage in the Setting of Renal Transplantation
Authors: Ruberto Franco , Lai Quirino , Piazzolla Mario , Poli Luca , Zullino Veronica , Diamantini Giulia , Brisciani Matteo , Giovanardi Francesco , Melandro Fabio , Quaresima Silvia , Rossi Massimo , Garofalo Manuela , Pugliese Francesco 
Published: January 2025

ABSTRACTBackgroundKidney transplantation (KT) is the most effective treatment for end‐stage renal disease. End‐ischemic hypothermic machine perfusion (EI‐HMP) has emerged as a promising method for preserving grafts before transplantation. This study aimed to compare graft function recovery in KT recipients of deceased brain‐death (DBD) grafts preserved with EI‐HMP versus static cold storage (SCS). The primary outcome was the rate of delayed graft function (DGF). Secondary outcomes included urine output, intensive care unit (ICU) stay, hospital stay duration, and survival rates.MethodsA retrospective, single‐center observational study was conducted at Sapienza University of Rome, analyzing 313 KT patients between January 2014 and September 2021. Patients were stratified into two groups based on graft preservation methods (EI‐HMP, n = 95; SCS, n = 218). A stabilized inverse probability treatment weighting (IPTW) method was employed to adjust for potential confounders.ResultsThere were no significant differences in DGF rates between the two groups (17.9% vs. 15.6% in SCS and EI‐HMP cases, respectively; p = 0.75). EI‐HMP group demonstrated a higher urine output on day 2 (p = 0.046), a shorter ICU stay (p < 0.0001), and a trend toward a shorter overall hospital stay (p = 0.07). No statistically significant differences were found between EI‐HMP and SCS cases in 1‐ and 3‐year overall survival rates (3.2% and 6.7% vs. 5.6% and 6.6%, respectively; log‐rank p = 0.53) or in death‐censored graft loss rates (5.4% and 8.9% vs. 5.7% and 7.3%, respectively; log‐rank p = 0.88). In a sub‐analysis of expanded criteria donors (ECD), EI‐HMP demonstrated a protective effect by reducing the risk of DGF (OR = 0.31, 95% CI = 0.09–0.95; p = 0.047).ConclusionEI‐HMP was associated with certain short‐term benefits, including increased urine output and reduced ICU stays, but showed no significant impact on long‐term survival outcomes. A reduction in DGF rates was observed only in the ECD subgroup. Randomized controlled trials are necessary to further investigate the long‐term clinical benefits of EI‐HMP.
Exploring Knowledge and Awareness of Sexual and Reproductive Health Rights Among Married Working Women: A Qualitative Study of Jaipur City
Authors: Sharma Anuradha , Jasrotia Amithy 
Published: January 2025

ABSTRACTKnowledge and awareness of Sexual and Reproductive Health/Rights (SRH/SRHR) have emerged as a prominent issue globally. Such knowledge leads to improved behaviors to minimize risks related to SRH as well as enhances women's autonomy regarding their fertility and sexuality. In the domain of SRH sociocultural norms and gender power dynamics play an even larger role in determining SRH outcomes. In India, despite progress in health, reproductive health remains a concern. SRH is still subjected to a social taboo in Indian society. Therefore, this study examines persistent challenges in acquiring knowledge of SRH among married working women, also, try to understand SRH issues from cultural and gender perspectives. An exploratory study conducted in Jaipur city (Rajasthan), included highly educated married working women (n = 104), using mixed‐methods with interviews and focus group discussions. Ethical guidelines ensured participant confidentiality and informed consent. Despite high educational and economic status significant gaps were found such as limited awareness of SRH concerns, family planning methods and SRHRs like rights to make informed decision, termination of pregnancy, maternity benefits, etc. This study emphasizes holistic approach to improve SRHR outcomes and advocated policy interventions for women of all sections of society.
Behaviour‐based pricing with overlapping ownership
Authors: Li Changying , Zhang Jianhu 
Published: January 2025

AbstractResearch has extensively explored why firms use their consumer information to price discriminate between repeat and new customers. This paper incorporates overlapping ownership and explores a more nuanced explanation for the practice. We isolate the different and interactive effects of overlapping ownership in different periods on equilibrium outcomes. In our explanation, firms use overlapping ownership to increase repeat purchase and customer retention and therefore may charge higher prices for both repeat and new customers, when they choose behaviour‐based pricing rather than uniform pricing. As a result, if the degree of overlapping ownership is not too small, behaviour‐based pricing benefits firms but hurts consumers.
Coumarin Analogues as Promising Anti‐Obesity Agents: In Silico Design, Synthesis, and In Vitro Pancreatic Lipase Inhibitory Activity
Authors: Yadav Nisha , Paul Atish T. 
Published: January 2025

ABSTRACTA set of coumarin‐3‐carboxamide analogues were designed, synthesized, and evaluated for their ability to impede pancreatic lipase (PL) activity. Out of all the analogues, 5dh and 5de demonstrated promising inhibitory activity against PL, as indicated by their respective IC50 values of 9.20 and 11.4 μM, as compared to Orlistat (IC50 = 0.97 μM). It was found that analogue 5dh inhibited PL in a competitive manner with an inhibition constant (Ki) of 4.504 μM. Additionally, the docking analysis validated the interactions between the analogue 5dh (MolDock score of −140.251 kcal/mol) and key amino acids in the active site, including Leu 153, Gly 76, Arg 256, His 151, Phe 77, and His 263. The inhibitory activity of these analogues was significantly correlated with their MolDock scores (Pearson's r = 0.6586). Finally, molecular dynamics simulation was also performed for 100 ns in order to elucidate the stability, confirmation and intermolecular interactions of the active analogue 5dh. The results of this investigation suggested that the complex maintained its stability despite the dynamic conditions exhibiting interactions with important amino acids. In summary, the outcomes indicated that the synthesized analogues exhibited the potential to inhibit PL activity.
Impact of hospital volume on survival in patients with locally advanced colon cancer – A Dutch population‐based study
Authors: de Nes L. C. F. , Tanis P. J. , Verhoeven R. H. , de Wilt J. H. W. , Vissers P. A. J. 
Published: January 2025

AbstractAimLocally advanced colon cancer (LACC) often necessitates complex prognosis‐determining treatment. This study investigated the impact of hospital volume on short‐ and long‐term outcomes following surgery for LACC.MethodData involving all patients with LACC categorized as clinical T4 and/or N2, between 2015 and 2019 in the Netherlands, were extracted from the Netherlands Cancer Registry. Hospitals were stratified into low volume (1–19 LACC resections per year), medium volume (20–29 LACC resections per year) and high volume (≥30 LACC resections per year). Data were analysed using Kaplan–Meier curves, logistic regression analysis and Cox‐regression models.ResultsA total of 49 298 patients were diagnosed with colon cancer, of whom 9206 (18.7%) had locally advanced disease. Of these 9206 patients, resection was performed in 8537 with a median age of 71 (interquartile range: 63–78) years. Patients were more likely to undergo laparoscopic procedures in high‐volume hospitals than in low‐volume hospitals (OR = 1.28, 95% CI: 1.12–1.46). No risk differences in anastomotic leakage or postoperative 90‐day mortality were observed according to hospital volume. Five‐year overall survival rates were comparable among high‐, medium‐ and low‐volume hospitals (58.7% vs. 58.0% vs. 60.0%, p = 0.62). Hospital volume was not associated with overall survival in multivariable analysis. Independent predictors of worse overall survival included older age, higher American Society of Anaesthesiologists score, emergency/urgent setting, anastomotic leakage, higher pTNM status, involved resection margins and no adjuvant chemotherapy.ConclusionDespite the complexity of surgical treatment, hospital volume was not associated with survival in LACC. Hospital volume might be an imperfect surrogate for quality assessment.