bluuebunny/crossref_metadata_2025_split huggingface dataset:
SUMOylation of RALY promotes vasculogenic mimicry in glioma cells via the FOXD1/DKK1 pathway
Authors: Cao Shuo , Wang Di , Wang Ping , Liu Yunhui , Dong Weiwei , Ruan Xuelei , Liu Libo , Xue Yixue , E Tiange , Lin Hongda , Liu Xiaobai 
Published: October 2023

AbstractHuman malignant gliomas are the most common and aggressive primary malignant tumors of the human central nervous system. Vasculogenic mimicry (VM), which refers to the formation of a tumor blood supply system independently of endothelial cells, contributes to the malignant progression of glioma. Therefore, VM is considered a potential target for glioma therapy. Accumulated evidence indicates that alterations in SUMOylation, a reversible post-translational modification, are involved in tumorigenesis and progression. In the present study, we found that UBA2 and RALY were upregulated in glioma tissues and cell lines. Downregulation of UBA2 and RALY inhibited the migration, invasion, and VM of glioma cells. RALY can be SUMOylated by conjugation with SUMO1, which is facilitated by the overexpression of UBA2. The SUMOylation of RALY increases its stability, which in turn increases its expression as well as its promoting effect on FOXD1 mRNA. The overexpression of FOXD1 promotes DKK1 transcription by activating its promoter, thereby promoting glioma cell migration, invasion, and VM. Remarkably, the combined knockdown of UBA2, RALY, and FOXD1 resulted in the smallest tumor volumes and the longest survivals of nude mice in vivo. UBA2/RALY/FOXD1/DKK1 axis may play crucial roles in regulating VM in glioma, which may contribute to the development of potential strategies for the treatment of gliomas.
Numerical simulation of multiscale fault systems with rate- and state-dependent friction
Authors: Gräser Carsten , Kornhuber Ralf , Podlesny Joscha 
Published: October 2023

AbstractWe consider the deformation of a geological structure with non-intersecting faults that can be represented by a layered system of viscoelastic bodies satisfying rate- and state-depending friction conditions along the common interfaces. We derive a mathematical model that contains classical Dieterich- and Ruina-type friction as special cases and accounts for possibly large tangential displacements. Semi-discretization in time by a Newmark scheme leads to a coupled system of nonsmooth, convex minimization problems for rate and state to be solved in each time step. Additional spatial discretization by a mortar method and piecewise constant finite elements allows for the decoupling of rate and state by a fixed point iteration and efficient algebraic solution of the rate problem by truncated nonsmooth Newton methods. Numerical experiments with a spring slider and a layered multiscale system illustrate the behavior of our model as well as the efficiency and reliability of the numerical solver.
Epidemiological analysis of drinking water-type fluorosis areas and the impact of fluorosis on children’s health in the past 40 years in China
Authors: Wang Feiqing , Li Yanju , Tang Dongxin , Zhao Jianing , Yang Bo , Zhang Chike , Su Min , He Zhixu , Zhu Xiaodong , Ming Dong , Liu Yang 
Published: October 2023

AbstractThis study analyzed the effect of China’s fluorosis prevention and control program, which has been in effect for more than 40 years, and the impact of fluorosis on children’s health. Relevant research studies were retrieved from the following online databases from the time of their inception to May 2022: PubMed, ScienceDirect, Embase, Cochrane, China National Knowledge Infrastructure, and Wanfang. The Review Manager 5.3 software was used in statistical analyses. This article included seventy studies: Thirty-eight studies reported the effect of improving water quality and reducing fluoride content, the incidence rate of dental fluorosis in children, and the level of urinary fluoride, and thirty-two studies reported the intelligence quotient (IQ) and health status of children. Following water improvement strategies, the fluoride levels in drinking water decreased significantly; urinary fluoride levels and dental fluorosis decreased significantly in children. With regard to the effect of fluorosis on the IQ of children, the results showed that the IQ of children in areas with a high fluoride of fluorosis was lesser than that in areas with a low fluoride, and this difference was significant. Based on the prevalence of dental fluorosis and its effect on the intelligence of children, it appears that reducing fluoride levels in drinking water and monitoring water quality are important strategies for the prevention and treatment of fluorosis.
Graphical abstract
Non-conventional treatments to control cork oak infection by Phytophthora cinnamomi
Authors: Bairrão Margarida , Trindade Manuel , Trindade Cândida Sofia , Marques-dos-Santos Cláudia , Ferreira Ricardo Boavida , Valdiviesso Teresa , Machado Helena 
Published: October 2023

AbstractCork oak (Quercus suber) is one of the most important forest species in Portugal and in the Mediterranean basin, but in recent decades a progressive decline has been registered in the montado ecosystem. The main phytopathogenic agent involved in this process, Phytophthora cinnamomi, has a high infection potential and survival ability, and became a serious threat to this ecosystem. Extensive areas of cork oak are infected in Portugal, being imperative to find new ways to control this disease. The complex lifecycle of this pathogen and the small number of effective agrochemicals currently authorized against Phytophthora spp., with phosphonates taking advantage, justifies this work where new products with antagonism against Oomycetes or biostimulant properties were tested. This study aimed to evaluate the efficacy of BLAD (a polypeptide with antifungal, anti-oomycete and biostimulant properties), potassium phosphonate and salicylic acid in Q. suber infected with P. cinnamomi, quantifying the severity of root lesions. BLAD, potassium phosphonate and salicylic acid treatments showed lower root lesion severities than the inoculated control, and proved to be effective as they provided a slower disease evolution. The lowest lesion severity in cork oak roots observed with BLAD treatments allowed us to consider BLAD as the most promising product among the three we tested.
Temporal patterns of stream biofilm in a mountain catchment: one-year monthly samplings across streams of the Orobic Alps (Northern Italy)
Authors: Bonacina Luca , Fornaroli Riccardo , Mezzanotte Valeria , Marazzi Francesca 
Published: October 2023

AbstractPeriphyton is the dominant primary producer in mountain streams and sustains the higher trophic levels. While certain periphyton groups, particularly diatoms, have received extensive study, the comprehensive characterization of the entire community has been largely neglected. This study aims to investigate the temporal pattern of biofilm in mountain streams characterized by different water thermal regimes. A one-year quantitative campaign, involving monthly samplings, was conducted in five subalpine streams in Northern Italy’s Orobic Alps to collect epilithic biofilm from a wide surface area. The total biomass was quantified and the periphyton was analyzed both for composition (diatoms, green algae, cyanobacteria, and red algae) and for pigments. Disturbance, water temperature, physico-chemical conditions, nutrients, substrate diversity, and light availability were assessed concurrently with biofilm samplings. Results show sharp biofilm variations over months. In all sites, the disturbance was the primary factor reducing biomass and pigment content. Annually, all sites experienced similar turnover in periphyton composition mainly associated with light and water temperature. Overall, the study indicates that frequent quantitative investigations of biofilm help understand intra-annual variations and identify key drivers. Such information is useful to understand the ecosystem processes and the food web dynamics.
The Importance of Representative Sampling for Home Range Estimation in Field Primatology
Authors: Jacobson Odd T. , Crofoot Margaret C. , Perry Susan , Hench Kosmas , Barrett Brendan J. , Finerty Genevieve 
Published: October 2023

AbstractUnderstanding the amount of space required by animals to fulfill their biological needs is essential for comprehending their behavior, their ecological role within their community, and for effective conservation planning and resource management. The space-use patterns of habituated primates often are studied by using handheld GPS devices, which provide detailed movement information that can link patterns of ranging and space-use to the behavioral decisions that generate these patterns. However, these data may not accurately represent an animal’s total movements, posing challenges when the desired inference is at the home range scale. To address this problem, we used a 13-year dataset from 11 groups of white-faced capuchins (Cebus capucinus imitator) to examine the impact of sampling elements, such as sample size, regularity, and temporal coverage, on home range estimation accuracy. We found that accurate home range estimation is feasible with relatively small absolute sample sizes and irregular sampling, as long as the data are collected over extended time periods. Also, concentrated sampling can lead to bias and overconfidence due to uncaptured variations in space use and underlying movement behaviors. Sampling protocols relying on handheld GPS for home range estimation are improved by maximizing independent location data distributed across time periods much longer than the target species’ home range crossing timescale.
The Importance of Grey and Qualitative Literature in Developing Domestic Violence and Abuse and Child Maltreatment Core Outcome Sets: A Brief Report
Authors: Powell Claire , Peeren Siofra , Ostrowska Ania , Adil Shehzore , Botevyle Jamie , Chesters Heather , Wolstencroft Jeanne , Yapp Emma , Feder Gene , Gilbert Ruth , Howarth Emma 
Published: October 2023

Abstract
Purpose
Core Outcome Sets (COS) are agreed sets of outcomes to be used in all trials that evaluate the effect of interventions. This report considers the added value of including grey and qualitative literature in a study to identify COSs of family-focused interventions for CM and DVA.

Methods
We identified outcomes of interventions for DVA or CM through systematically searching 12 academic databases and 86 organisation websites, leading to the inclusion of 485 full-text reports across 6 reviews. We developed a candidate outcome longlist comprising 347 extracted outcomes.

Results
We identified 87% (282/347) of candidate outcomes from the grey and qualitative literature, and 37% (127/347) from the trial literature. Of the candidate outcomes on the longlist, 22% (75/347) were identified solely from the grey or qualitative literature and 7% (26/347) from trial literature. Three of the eight outcomes in the final core outcome sets may have been missed if grey or qualitative literature had not been searched.

Conclusions
The qualitative and grey literature adds DVA and CM outcomes that are relevant to survivor perspectives but not reported in trials; this had an impact on the final COSs. It is important for COS developers to consider what they may be missing if they do not search the qualitative and grey literature.
Quantitative analysis of selenium species in the edible parts of cabbage, carrot, tomato and green pea treated with selenate-enriched irrigation water
Authors: Ragályi Péter , Takács Tünde , Soós Áron , Kovács Béla , Dernovics Mihály , Lončarić Zdenko , Dobosy Péter , Záray Gyula , Rékási Márk 
Published: October 2023

Abstract
Background and aims
Selenium (Se)-biofortified foods are able to compensate for Se deficient diets, but the proportion of different Se species varies among plant species, and the bioavailability of Se species also varies. This study aims to examine the Se species composition of four vegetables.

Methods
The effect of Se-enriched irrigation water on the concentrations of Se species in the edible parts of cabbage, carrots, tomatoes and green peas grown on different soil types was investigated and quantified using HPLC-ICP-MS with either standard addition or isotope dilution.

Results
Cabbage leaf and carrot root contained a high proportion (88 and 92%, respectively) of selenate (SeVI), while in tomato fruit and green pea seed selenomethionine (SeMet) was found in the highest proportion (33% and 48%, respectively), but elemental Se was also detected. The elemental Se concentration of peas was significantly higher on sandy soil (2.29 mg kg−1) than on sandy silt (1.38 mg kg−1) or silt soil (1.34 mg kg−1). In 100 g of fresh edible parts, the largest amount of Se species was found in cabbage leaves, but organic Se in the form of SeMet was the most abundant in green peas, being significantly higher than in carrots or tomatoes. Tomatoes contained a nearly three-fold ratio of organic Se to inorganic Se, the latter being mostly comprised of the elemental form, which is unusually high in vegetables. The highest rate of unknown Se species exceeded 66% in the case of peas, while the highest identification rate (> 95%) of Se could be achieved in tomato.

Conclusion
Tomatoes converted the absorbed Se into organic Se in the highest ratio, while green peas had the highest amount of organic Se in fresh biomass, so biotransformation was the most effective for these vegetables without any significant biomass reduction. These vegetables may be adapted to soils with a higher Se content.
A Deeper Analysis of Volumetric Relightable Faces
Authors: Rao Pramod , Mallikarjun B. R. , Fox Gereon , Weyrich Tim , Bickel Bernd , Pfister Hanspeter , Matusik Wojciech , Zhan Fangneng , Tewari Ayush , Theobalt Christian , Elgharib Mohamed 
Published: October 2023

AbstractPortrait viewpoint and illumination editing is an important problem with several applications in VR/AR, movies, and photography. Comprehensive knowledge of geometry and illumination is critical for obtaining photorealistic results. Current methods are unable to explicitly model in 3D while handling both viewpoint and illumination editing from a single image. In this paper, we propose VoRF, a novel approach that can take even a single portrait image as input and relight human heads under novel illuminations that can be viewed from arbitrary viewpoints. VoRF represents a human head as a continuous volumetric field and learns a prior model of human heads using a coordinate-based MLP with individual latent spaces for identity and illumination. The prior model is learned in an auto-decoder manner over a diverse class of head shapes and appearances, allowing VoRF to generalize to novel test identities from a single input image. Additionally, VoRF has a reflectance MLP that uses the intermediate features of the prior model for rendering One-Light-at-A-Time (OLAT) images under novel views. We synthesize novel illuminations by combining these OLAT images with target environment maps. Qualitative and quantitative evaluations demonstrate the effectiveness of VoRF for relighting and novel view synthesis, even when applied to unseen subjects under uncontrolled illumination. This work is an extension of Rao et al. (VoRF: Volumetric Relightable Faces 2022). We provide extensive evaluation and ablative studies of our model and also provide an application, where any face can be relighted using textual input.
Umwelt Collapse: The Loss of Umwelt-Ecosystem Integration
Authors: Maran Timo 
Published: October 2023

AbstractJakob von Uexküll’s umwelt theory opens new perspectives for understanding animal extinction. The umwelt is interpreted here as a sum of structural correspondences between an animal’s subjective experience, ecosystem, physiology, and behaviour. The global environmental crisis disturbs these meaning-connections. From the umwelt perspective, we may describe extinction as umwelt collapse: The disintegration of an animal’s umwelt resulting from the cumulative errors in semiotic processes that mediate an organism and ecosystem. The loss of umwelt-ecosystem integration disturbs “ecological memory,” which provides the ecosystem with adaptive modelling and self-design capacities. Making a distinction between core and mediated umwelts, and describing different types of umwelt collapse, are suitable methods for more detailed analysis. The concept of umwelt collapse enables the reinterpretation of extinction, from an internal perspective, as a semiotic breakdown. Such an approach may help us map scenarios of animal extinction, and may lead to successful compensation strategies in adapting to environmental change.
Down-regulation of DPP4 by TGFβ1/miR29a-3p inhibited proliferation and promoted migration of ovarian cancer cells
Authors: Liu Chong , Gao Zhao-Wei , Liu Ying-Qi , Yang Lan , Wu Xia-Nan , Dong Ke , Zhu Xiao-Ming 
Published: October 2023

Abstract
Objective
To explore the DPP4 expression changes and functions in ovarian cancer (OV), as well as the regulation mechanism for DDP4.

Methods
GEPIA2, GSE18520, GSE26712 and UALCAN were used to analyze differences in DPP4 expression between OV tumors and control tissues. Serum DPP4 levels were measured by ELISA. The prognostic values of DPP4 were evaluated using a Kaplan–Meier (KM) plotter. Small interfering RNA was used for DPP4 knockdown in OVCAR-3 and SKOV-3 cells. CCK-8 and scratch healing assays were used to determine the cells’ proliferation and migration abilities. Flow cytometry (FCM) was used to detect the cell cycle and apoptosis. A dual-luciferase assay was designed to confirm the regulatory effect of miR-29a-3p on DPP4.

Results
The expressions of DPP4 mRNA and protein were decreased in OV tumor tissues. Serum DPP4 levels decreased in OV patients. KM plotter analysis showed correlation between high DPP4 expression and a poor prognosis in OV patients. By targeting knockdown of DPP4, we found that OVCAR-3 and SKOV-3 cells’ proliferation was inhibited, while cell’s migration ability was significantly promoted. FCM analysis showed that DPP4 knockdown induced a decrease in the S phase. Furthermore, DPP4 was shown to be downregulated by miR-29a-3p and TGFβ1 in OVCAR-3 cells, and miR-29a-3p expression was upregulated by TGFβ1. The effects of miR-29a-3p and TGFβ1 on OVCAR-3 cells’ biological behaviors were consistent with DPP4 knockdown.

Conclusion
DPP4 was downregulated in OV patients. DPP4 knockdown significantly inhibited OVCAR-3 and SKOV-3 cell proliferation and promoted cell migration. DDP4 can be downregulated by TGFβ1 through the upregulation of miR-29a-3p in OV cells.
On the theory of Bergman spaces on homogeneous Siegel domains
Authors: Calzi Mattia , Peloso Marco M. 
Published: October 2023

AbstractWe consider mixed-norm Bergman spaces on homogeneous Siegel domains. In the literature, two different approaches have been considered and several results seem difficult to be compared. In this paper, we compare the results available in the literature and complete the existing ones in one of the two settings. The results we present are as follows: natural inclusions, density, completeness, reproducing properties, sampling, atomic decomposition, duality, continuity of Bergman projectors, boundary values, and transference.
Effect of Geosynthetics on Swell Reduction During an Extreme Rainfall Event
Authors: Saily Mohit , Gratchev Ivan 
Published: October 2023

AbstractExpansive (reactive) soils can swell during rainfall causing damage to engineering structures. This paper seeks to investigate the effect of geosynthetic material on the swell reduction of such soils. A series of large-scale long-term soil column experiments were conducted on a very high plasticity reactive soil under simulated rainfall conditions. Non-woven geosynthetics combined with geogrid and woven geosynthetics were used as primary geosynthetic material to provide reinforcement and drainage for the soil mass. The obtained results revealed that these geosynthetics did not improve the California Bearing Ratio values of the soil. However, when placed in the soil mass, the geosynthetic materials greatly reduced the total swell of the soil. Visual observations and the experimental data on water content, suction, and vertical displacements indicated that the geosynthetics provided better drainage of the soil mass. This limited the time of water–soil interaction, thus reducing the total swell of the soil.
High conductive copper alloys for additive manufacturing
Authors: Fiedler T. , Jähnig Domingues M. , Winter C. , Rösler J. 
Published: October 2023

AbstractFor applications where high thermal and/or electrical conductivity combined with reasonably high strength is required, copper alloys may be used. Although many different alloys were already developed in the past, additive manufacturing like laser powder bed fusion (PBF-LB/M) opens up new possibilities for alloy development, mainly driven by the very high cooling rates. This allows for the usage of precipitation-hardened alloys with compositions exceeding the maximum solubility. The present work focuses on the investigation of a well-known CuCr1Zr alloy as well as CuZr alloys with 1 and 2 wt.% Zr. For a fast, resource-efficient screening and demonstration of feasibility, the investigated alloys were not printed from powder. Instead, solid sheets were partially re-melted in a PBF-LB/M machine to obtain a microstructure similar to the printed state. This rapid-solidification microstructure is investigated, and precipitates with a size 50 nm or even smaller are found. After subsequent aging heat treatments, the hardness of the alloys exceeds the maximum hardness achievable with conventional manufacturing methods (excluding work hardening). The investigations in this work revealed the great hardening potential of these alloys for usage in the PBF-LB/M process.
Mucosal immune responses to Ichthyophthirius multifiliis in the ocular mucosa of rainbow trout (Oncorhynchus mykiss, Walbaum), an ancient teleost fish
Authors: Kong Weiguang , Ding Guangyi , Cheng Gaofeng , Yang Peng , Xu Zhen 
Published: October 2023

AbstractThe eye, as a specialized visual organ, is directly exposed to the external environment, and, therefore, it faces constant challenges from external pathogenic organisms and toxins. In the ocular mucosa (OM) of mammals, mucosal-associated lymphoid tissues (MALTs) constitute the primary line of defense. However, the immune defense role of the OM remains unknown in aquatic vertebrates. To gain insights into the immune processes within the OM of teleost fish, we developed an infection model of rainbow trout (Oncorhynchus mykiss) OM using a parasite, Ichthyophthirius multifiliis (Ich). Immunofluorescence, qPCR, and H&E staining revealed that Ich successfully infiltrates the OM of rainbow trout, leading to pathological structural changes, as evidenced by A&B staining. Importantly, the qPCR results indicate an up-regulation of immune-related genes following Ich infection in the OM. Moreover, transcriptome analyses were conducted to detect immune responses and impairments in eye function within the OM of rainbow trout with Ich infection. The results of the transcriptome analysis that Ich infection can cause an extensive immune response in the OM, ultimately affecting ocular function. To the best of our knowledge, our findings represent for the first time that the teleost OM could act as an invasion site for parasites and trigger a strong mucosal immune response to parasitic infection.