bluuebunny/crossref_metadata_2025_split huggingface dataset:
scSNV-seq: high-throughput phenotyping of single nucleotide variants by coupled single-cell genotyping and transcriptomics
Authors: Cooper Sarah E. , Coelho Matthew A. , Strauss Magdalena E. , Gontarczyk Aleksander M. , Wu Qianxin , Garnett Mathew J. , Marioni John C. , Bassett Andrew R. 
Published: January 2024

AbstractCRISPR screens with single-cell transcriptomic readouts are a valuable tool to understand the effect of genetic perturbations including single nucleotide variants (SNVs) associated with diseases. Interpretation of these data is currently limited as genotypes cannot be accurately inferred from guide RNA identity alone. scSNV-seq overcomes this limitation by coupling single-cell genotyping and transcriptomics of the same cells enabling accurate and high-throughput screening of SNVs. Analysis of variants across the JAK1 gene with scSNV-seq demonstrates the importance of determining the precise genetic perturbation and accurately classifies clinically observed missense variants into three functional categories: benign, loss of function, and separation of function.
Early tirofiban versus heparin for bridging dual antiplatelet therapy in patients undergoing coronary endarterectomy combined with coronary artery bypass grafting: a multicenter randomized controlled trial protocol (the THACE-CABG trial)
Authors: Chen Liang , Gao Ming-Xin , Du Xin , Wang Chi , Yu Wen-Yuan , Liu Hong-Li , Ding Xiao-Hang , Wang Bo-Lin , Zhang Kui , Xu Dong , Han Zhen , Xie Bao-Dong , Dong Ran , Yu Yang 
Published: January 2024

Abstract
Background
For complete revascularization, patients with diffuse coronary artery disease should have a coronary endarterectomy and a coronary artery bypass graft (CE-CABG). Sadly, CE can lead to a lack of endothelium, which raises the risk of thrombotic events. Even though daily dual antiplatelet therapies (DAPT) have been shown to reduce thrombotic events, the risk of perioperative thrombotic events is high during the high-risk period after CE-CABG, and there is no consistent protocol to bridge DAPT. This trial aims to compare safety and efficacy between tirofiban and heparin as DAPT bridging strategies after CE-CABG.

Methods
In phase I, 266 patients undergoing CE-CABG will be randomly assigned to tirofiban and heparin treatment groups to compare the two treatments in terms of the primary safety endpoint, chest tube drainage in the first 24 h. If the phase I trial shows tirofiban non-inferiority, phase II will commence, in which an additional 464 patients will be randomly assigned. All 730 patients will be studied to compare major cardiovascular and cerebrovascular events (MACCEs) between the groups in the first 30 days after surgery.

Discussion
Given the possible benefits of tirofiban administration after CE-CABG, this trial has the potential to advance the field of adult coronary heart surgery.

Trial registration
chictr.org.cn, ChiCTR2200055697. Registered 6 January 2022. https://www.chictr.org.cn/com/25/showproj.aspx?proj=149451. Current version: 20,220,620.
The Transdiagnostic Intervention for Sleep and Circadian Dysfunction (TranS-C) for serious mental illness in community mental health part 3: study protocol to evaluate sustainment in a hybrid type 2 effectiveness-implementation cluster-randomized trial
Authors: Sarfan Laurel D. , Agnew Emma R. , Diaz Marlen , Cogan Ashby , Spencer Julia M. , Esteva Hache Rafael , Wiltsey Stirman Shannon , Lewis Cara C. , Kilbourne Amy M. , Harvey Allison G. 
Published: January 2024

Abstract
Background
Although research on the implementation of evidence-based psychological treatments (EBPTs) has advanced rapidly, research on the sustainment of implemented EBPTs remains limited. This is concerning, given that EBPT activities and benefits regularly decline post-implementation. To advance research on sustainment, the present protocol focuses on the third and final phase—the Sustainment Phase—of a hybrid type 2 cluster-randomized controlled trial investigating the implementation and sustainment of the Transdiagnostic Intervention for Sleep and Circadian Dysfunction (TranS-C) for patients with serious mental illness and sleep and circadian problems in community mental health centers (CMHCs). Prior to the first two phases of the trial—the Implementation Phase and Train-the-Trainer Phase—TranS-C was adapted to fit the CMHC context. Then, 10 CMHCs were cluster-randomized to implement Standard or Adapted TranS-C via facilitation and train-the-trainer. The primary goal of the Sustainment Phase is to investigate whether adapting TranS-C to fit the CMHC context predicts improved sustainment outcomes.

Methods
Data collection for the Sustainment Phase will commence at least three months after implementation efforts in partnering CMHCs have ended and may continue for up to one year. CMHC providers will be recruited to complete surveys (N = 154) and a semi-structured interview (N = 40) on sustainment outcomes and mechanisms. Aim 1 is to report the sustainment outcomes of TranS-C. Aim 2 is to evaluate whether manipulating EBPT fit to context (i.e., Standard versus Adapted TranS-C) predicts sustainment outcomes. Aim 3 is to test whether provider perceptions of fit mediate the relation between treatment condition (i.e., Standard versus Adapted TranS-C) and sustainment outcomes. Mixed methods will be used to analyze the data.

Discussion
The present study seeks to advance our understanding of sustainment predictors, mechanisms, and outcomes by investigating (a) whether the implementation strategy of adapting an EBPT (i.e., TranS-C) to the CMHC context predicts improved sustainment outcomes and (b) whether this relation is mediated by improved provider perceptions of treatment fit. Together, the findings may help inform more precise implementation efforts that contribute to lasting change.

Trial registration
ClinicalTrials.gov identifier: NCT05956678. Registered on July 21, 2023.
Flubendazole carbonyl reduction in drug-susceptible and drug-resistant strains of the parasitic nematode Haemonchus contortus: changes during the life cycle and possible inhibition
Authors: Rychlá Nikola , Navrátilová Martina , Kohoutová Eliška , Raisová Stuchlíková Lucie , Štěrbová Karolína , Krátký Josef , Matoušková Petra , Szotáková Barbora , Skálová Lenka 
Published: January 2024

AbstractCarbonyl-reducing enzymes (CREs) catalyse the reduction of carbonyl groups in many eobiotic and xenobiotic compounds in all organisms, including helminths. Previous studies have shown the important roles of CREs in the deactivation of several anthelmintic drugs (e.g., flubendazole and mebendazole) in adults infected with the parasitic nematode Haemonchus contortus, in which the activity of a CRE is increased in drug-resistant strains. The aim of the present study was to compare the abilities of nematodes of both a drug-susceptible strain (ISE) and a drug-resistant strain (IRE) to reduce the carbonyl group of flubendazole (FLU) in different developmental stages (eggs, L1/2 larvae, L3 larvae, and adults). In addition, the effects of selected CRE inhibitors (e.g., glycyrrhetinic acid, naringenin, silybin, luteolin, glyceraldehyde, and menadione) on the reduction of FLU were evaluated in vitro and ex vivo in H. contortus adults. The results showed that FLU was reduced by H. contortus in all developmental stages, with adult IRE females being the most metabolically active. Larvae (L1/2 and L3) and adult females of the IRE strain reduced FLU more effectively than those of the ISE strain. Data from the in vitro inhibition study (performed with cytosolic-like fractions of H. contortus adult homogenate) revealed that glycyrrhetinic acid, naringenin, mebendazole and menadione are effective inhibitors of FLU reduction. Ex vivo study data showed that menadione inhibited FLU reduction and also decreased the viability of H. contortus adults to a similar extent. Naringenin and mebendazole were not toxic at the concentrations tested, but they did not inhibit the reduction of FLU in adult worms ex vivo.
An improved ACO based service composition algorithm in multi-cloud networks
Authors: Bei Liu , Wenlin Li , Xin Su , Xibin Xu 
Published: January 2024

AbstractIn recent years, with the rapid development of mobile communication networks, some new services such as cloud virtual reality, holographic communication, and etc. continue to emerge. Service composition has been researched in cloud computing. however, as the fast development of edge clouds, the service components can be deployed on the edge clouds to reduce the composition latency, so the more flexible and intelligent service composition algorithms are urgently need to study. Based on this, we propose a service composition strategy under the multi-cloud environment, and we propose an ant colony optimization algorithm (ACO) based on the multi-pheromone mechanism to optimize the quality of service (QoS). To avoid the occurrence of local optima, we further introduce the mutation operation of the genetic algorithm. Finally, the simulation results show that the proposed algorithm can achieve better QoS parameters such as latency and response time while ensuring the stability of services.
Metabolic modulation of Basil (Ocimum basilicum L.): an insight into growth, metabolomics and antioxidant activity under varying temperature and light conditions
Authors: Ryu Da Hye , Cho Jwa Yeong , Hamayun Muhammad , Lee Dong Gyu , Kim Ho-Youn 
Published: January 2024

Abstract
Background
Temperature and light are two critical environmental stimuli that greatly impact our agricultural industry. We explored the effects of varying temperature and light conditions on the metabolomics of basil, a plant widely used in cuisine and for medicinal purposes worldwide. Basil plants were subjected to elevated temperatures of 30 °C, 35 °C, and 40 °C, along with two light intensities (D11 and D23).

Results
Our results showed that the maximum shoot length (42.98 cm) was achieved at T30 and D23, the maximum leaf area (6.92 cm) at T30/D11, and the highest number of leaves (472) at T40/D11. However, at the higher temperature of T40, the number of leaves increased, but shoot length and leaf size significantly decreased (p < 0.05). Maxim shoot biomass (81 gm) and root biomass (91.2 gm) was recorded at T35/D23 treatments. Metabolomic studies of basil revealed that rosmarinic acid (RA), and linalool and eugenol were the major phenolic and volatile organic compounds (VOCs) under different growth conditions. RA levels were higher at 30 °C, correlated with elevated shikimate levels, indicating a carbon supply through the TCA cycle. In contrast, the growth conditions of 35 °C/D11 (μmol/m2/d) up-regulated the glyoxylate cycle (fumaric acid and malic acid) as part of stomatal closure, preventing ROS accumulation and resulting in increased VOC emission. Exposure to the higher temperature of 40 °C induced higher amino acid accumulation, improving temperature stresstolerance.

Conclusions
This study suggests that elevated temperature and light intensity affected the overall health of basil plants by modulating underlying growth mechanisms. Furthermore, elevated temperature plays a vital role in plant defense mechanisms by mediating the production of secondary metabolites associated with plant defense responses.

Graphical Abstract
PIONEERS: a 6DoF motion sensor to measure rotation and tides in the Solar System
Authors: Filice Valerio , Le Maistre Sébastien , Dehant Véronique , Van Hoolst Tim , Bernauer Felix , Garcia Raphaël F. 
Published: January 2024

AbstractObservation of rotation variations and tides provides constraints on the interior properties of celestial bodies. Both can be precisely measured with a 6DoF (Degrees of Freedom) motion sensor placed on their surface. This type of instrument measures rotation rates and linear accelerations in a large frequency band, which includes the frequencies involved in the tides and rotation variations. A novel sensor under development aims to measure rates and accelerations with an amplitude spectral density of $${2}\,\upmu \textrm{rad}\, \textrm{s}^{-1} \textrm{H}\textrm{z}^{-1/2}$$

2

μ
rad

s

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1

H

z

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/
2

and $${20}\,\upmu \textrm{m}\,\textrm{s}^{-2} \textrm{H}\textrm{z}^{-1/2}$$

20

μ
m

s

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2

H

z

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/
2

respectively in its compact version and three orders of magnitude better ($${5}\,\textrm{nrad}\, \textrm{s}^{-1} \textrm{H}\textrm{z}^{-1/2}$$

5

nrad

s

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1

H

z

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1
/
2

and $${10}\,\textrm{pm}\,\textrm{s}^{-2} \textrm{H}\textrm{z}^{-1/2}$$

10

pm

s

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z

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2

, respectively) with its high-performance version. Here, we compare these instrument performances with the precision required to measure rotation and tides in order to improve our knowledge of the interior of nine celestial bodies identified as targets for future space missions: Dimorphos, Phobos, Europa, Io, Titan, Enceladus, Triton, the Moon and Mars. Results indicate that Phobos, the Moon, and Mars cannot be investigated with the compact model, but that the interior of the other bodies can be constrained through measurements of rotation rate, and/or centrifugal acceleration, and/or tidal acceleration. We also find that the high-performance prototype instrument is suitable for acceleration measurements for all nine bodies, but not adequate for inferring interior constraints from rotation rate measurements for Triton, the Moon, and Mars. The signatures of the interior in the rotation rate and centrifugal and tidal accelerations also provide scientific requirements for future developments of 6DoF motion sensors for these nine bodies.
Graphical Abstract
An interest-driven creator English course for developing Chinese private college students’ listening and speaking proficiency under a blended setting
Authors: Wang Yongkang , Chen Pengfei 
Published: January 2024

AbstractThe primary objective of this study is to enhance the English language proficiency of undergraduate students pursuing bachelor's degree programs. This is achieved through the development of a mandatory English course based on Interest-driven creator (IDC) principles within a blended learning environment. The IDC course was implemented over a 16-week period, consisting of 16 lessons, at a private college in China. To evaluate the impact of this course, a quasi-experimental design was employed to analyse the language learning outcomes. The performance of two groups, the experimental group (EG) and the control group (CG), was assessed using the Exam English (standardized according to CEFR) as both a pretest and posttest measure. Additionally, a semi-structured interview was conducted to gather valuable insights into the students' learning experiences. The findings of this study indicate that the blended learning approach effectively improved the students' English proficiency in listening and speaking.
Students' digital technology attitude, literacy and self-efficacy and their effect on online learning engagement
Authors: Getenet Seyum , Cantle Robert , Redmond Petrea , Albion Peter 
Published: January 2024

AbstractThis study utilised students' online engagement, digital technology attitude, digital literacy, and self-efficacy theories to develop and test a model connecting these factors within a regional university in Australia. A field survey collected data from 110 first-year students. AMOS 28 was employed for measurement and structural model path analysis. The study initially examined the impact of students' attitudes and digital literacy on their self-efficacy. Subsequently, the effects of self-efficacy on five dimensions of online engagement were assessed: social, collaborative, cognitive, behavioural, and emotional. The findings indicated that positive student attitudes and digital literacy significantly contributed to self-efficacy, which, in turn, positively affected the engagement dimensions. This suggests that when designing and facilitating online, blended, or technology-enhanced courses in higher education, educators should pay attention to various elements of engagement. The study highlights the importance of considering students' attitudes and digital literacy in fostering self-efficacy and enhancing online learning engagements. Further research and implications for future studies are also recommended.
Application of unilateral biportal endoscopy technique to resect a thoracic spinal intradural extramedullary meningioma: technical report and review of the literature
Authors: Shao Rongxue , Cheng Wei , Zhang Wei , Liang Jiaming , Ruan Liqi , Zhu Chengyue , Pan Hao 
Published: January 2024

Abstract
Background
Meningiomas are common intradural extramedullary spinal tumors, which arise from arachnoid cap cells in the leptomeninges surrounding the brain or spinal cord. Sensory and motor dysfunction as well as pain were the most common presenting symptoms. Surgical resection remains the primary treatment for spinal intradural extramedullary meningiomas. Traditionally, spinal meningiomas excision requires longer skin incision, bilateral subperiosteal muscle stripping, and total laminectomy. We report a new technique for the treatment of thoracic spinal intradural extramedullary meningioma, which involves the use of unilateral biportal endoscopy (UBE) technique to resect the tumor, and reviewed and analyzed relevant literature.

Case presentation
A 69-year-old female patient presented with back pain accompanied by slowly progressive lower limb paresis, and severe pain in the right lower limb. Magnetic resonance imaging suggests a thoracic spinal intradural extramedullary meningioma. She underwent meningioma resection using UBE technique with complete recovery at the follow-up examination 3 months after surgery.

Conclusion
This case confirmed the UBE technique can be a good choice for resection of spinal intradural extramedullary meningioma.
Pseudoaneurysms embolization with glue via percutaneous direct puncture: a multicenter experience on 54 patients
Authors: Giurazza Francesco , Ierardi Annamaria , Marra Paolo , Lucatelli Pierleone , Corvino Fabio , Pane Francesco , Sironi Sandro , Carrafiello Gianpaolo , Loffroy Romaric , Niola Raffaella 
Published: January 2024

Abstract
Background
This retrospective multicentric study aims to report on technical safety and effectiveness of pseudoaneurysms embolization with glue (N-butyl cyanoacrylate) adopting a percutaneous direct puncture approach.

Results
Fifty-four patients data were collected from five centers.
All patients at the time of treatment presented with unruptured PAs and were hemodynamically stable.
True aneurysms and lesions treated with embolics other than glue were excluded.
Pseudoaneurysms diagnosis was based on CT and anamnestic data; initial investigation with digital-subtracted arteriography was acquired in all cases; then, percutaneous embolizations were performed in the angio-suite (ultrasound, fluoroscopy, ConeBeam CT guidance) or in CT.
Technical success was considered as complete pseudoaneurysm embolization at final imaging with sole percutaneous strategy, without need for additional endovascular embolization.
Clinical success was intended as pseudoaneurysm resolution within one week follow-up with stabilization or restored clinical conditions.
Pseudoaneurysms origins were traumatic (57.4%), inflammatory (24.1%) or spontaneous (18.5%); 39 patients (72.2%) were symptomatic, presenting with pain and/or pulsatile mass.
Mean lesions diameter was 19.3 mm (range: 7–30); pseudoaneurysms were located in abdomen (48.1%), limbs (42.6%) and thorax (9.3%).
Coagulation function was impaired in 16.6% and 48.1% was under antiplatelets/anticoagulation therapy.
In 16.6% the percutaneous approach followed previous treatments failure.
The image-guidance modality for percutaneous puncture was most often ultrasound combined with fluoroscopy (38%).
Clinical success was obtained in all patients while technical success occurred in 94.4% because 3 patients required an additional endovascular embolization.
Complications were registered in 14.8%, all of low grade without clinical sequelae neither prolonged recovery (7 non target embolizations, 1 post-embolization syndrome).

Conclusions
In this study, pseudoaneurysms embolization with glue via percutaneous direct puncture was safe and effective with a low rate of minor complications.
Chemometric method development for the determination of naringin and verapamil
Authors: Sharma Sadhana , Shrestha Bhupendra , Bhuyan Nihar Ranjan , Majumdar Subhabrota , Chowdhury Sailee , Mazumder Rana 
Published: January 2024

Abstract
Background
By resolving complicated spectra from drug combinations, chemometric techniques are valuable for multi-component investigation. The capacity to properly estimate combinations of components without separating drugs from their mixture is one of the benefits of chemometric analysis approaches over traditional analytical methods. These approaches are easy to use and sensitive even to the lowest concentrations. They are also practical, affordable, and cost-effective. In the current study, the chemometric aided spectrophotometric approach was used to evaluate the two drugs naringin and verapamil. The approach is multidimensional and based on chemometrics, which includes an orthogonal partial least square method that is a new refinement of the partial least squares regression analysis method. With this technique, no conversions are made to the spectrum that overlaps the two drugs. The tools UV-PROBE, SIMCA version 17, and excel were used to process the chemometric data.

Results
According to results from an orthogonal partial least square model, the mean percent recovery and relative standard deviation for the combination of verapamil with naringin were 100.80/1.19 and 100.836/1.35, respectively.The calibration model was used to predict known synthetic mixtures.This method shows good consistency in recovery ranging between 98.92 and 103.59% for VER and from 96.21 to 101.84% NAR. As saying the synthetic mixture revealed that it had a high percentage of purity.

Conclusions
The proposed chemometric method can estimate the quantitative amount of pharmaceuticals based on their dosage forms. This approach meets the requirements for the international conference on harmonization's (ICH) analytical criteria, such as precision and accuracy.

Graphical Abstract
A rare enterouterine fistula between uterine leiomyoma and sigmoid colon in a nulliparous woman: a case report
Authors: Shruthi M. Maivizhi , Sahoo Biswajit , Jena Saubhagya Kumar , Sethi Pruthwiraj , Mitra Subarna , Nayak Manoj Kumar , Jha Shilpy , Bag Nerbadyswari Deep , Sarvazna Laveti Sai 
Published: January 2024

Abstract
Background
The commonest benign neoplasm encountered in women of reproductive age group is uterine leiomyoma which usually presents with abnormal uterine bleeding, chronic pelvic pain, urinary disturbances, urinary retention, constipation, and dyspareunia. Uterine leiomyoma presenting with bowel fistulization is a rare presentation. To our knowledge and based on a thorough literature search, no previous reports of uterine leiomyoma showing spontaneous fistulous communication with the sigmoid colon have been reported.

Case presentation
A nulliparous woman in her early 30s presented with complaints of lower abdominal pain, abdominal fullness, pus discharge from the vagina, and difficulty in respiration. On contrast-enhanced computed tomography (CECT), a large heterogeneously enhancing mass was seen in the rectouterine space showing air-fluid levels, with a fistulous communication with the adjacent sigmoid colon. Histopathological examination proved the lesion to be a uterine leiomyoma.

Conclusion
The chance of fistulization with the bowel should be considered whenever a uterine mass with an air-fluid level is encountered.