bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Neurobeachin‐like 2 protein (NBEAL2) controls the homeostatic level of the ribosomal protein RPS6 in mast cells
Authors: Wegner Philine , Drube Julia , Ziegler Lisa , Strotmann Birgit , Marquardt Raphaela , Küchler Claudia , Groth Marco , Nieswandt Bernhard , Andreas Nico , Drube Sebastian 
Published: January 2024

AbstractThe Beige and Chediak‐Higashi (BEACH) domain‐containing, Neurobeachin‐like 2 (NBEAL2) protein is a molecule with a molecular weight of 300 kDa. Inactivation of NBEAL2 by loss‐of‐function mutations in humans as well as deletion of the Nbeal2 gene in mice results in functional defects in cells of the innate immune system such as neutrophils, NK‐cells, megakaryocytes, platelets and of mast cells (MCs). To investigate the detailed function of NBEAL2 in murine MCs we generated MCs from wild type (wt) and Nbeal2−/− mice, and deleted Nbeal2 by CRISPR/Cas9 technology in the murine mast cell line MC/9. We also predicted the structure of NBEAL2 to infer its function and to examine potential mechanisms for its association with interaction partners by using the deep learning‐based method RoseTTAFold and the Pymol© software. The function of NBEAL2 was analysed by molecular and immunological techniques such as co‐immunoprecipitation (co‐IP) experiments, western blotting, enzyme‐linked immunosorbent assay and flow cytometry. We identified RPS6 as an interaction partner of NBEAL2. Thereby, the NBEAL2/RPS6 complex formation is probably required to control the protein homeostasis of RPS6 in MCs. Consequently, inactivation of NBEAL2 leads to accumulation of strongly p90RSK‐phosphorylated RPS6 molecules which results in the development of an abnormal MC phenotype characterised by prolonged growth factor‐independent survival and in a pro‐inflammatory MC‐phenotype.
Discrete cooperative coverage location models with alternative facility types in a probabilistic setting
Authors: Michopoulou Maria , Giannikos Ioannis 
Published: January 2024

AbstractIn this paper, we discuss discrete location problems where the objective is to locate a given number of facilities of different types in order to appropriately cover a given set of demand points. The coverage provided to each demand point is the result of the cooperation among the located facilities. The different types of facility refer to the coverage radius or quality of coverage that each type may provide. We present a non‐linear formulation of the problem where the objective is to maximize the percentage of demand points that are appropriately covered. We then show how the model can be linearized based on a representation of probabilities through a network structure. To address large instances of the problem, we introduce a genetic algorithm (GA) that is based on the representation of the solution by two chromosomes. Computational experiments over a wide range of randomly generated problems indicate the GA clearly outperforms CPLEX, especially in larger instances.
Sintering behavior, crystal structure, and microwave dielectric properties of a novel NaY9Si6O26 ceramic
Authors: Li Yingxiang , Liang Deyin , Zhang Xing , Xiong Zhe , Tang Bin , Si Feng , Fang Zixuan , Shi Zitao , Chen Jingjing , Wang Fei , Li Hao 
Published: January 2024

AbstractThis study investigates NaY9Si6O26 ceramics prepared through the solid‐phase method, focusing on their microwave dielectric properties and crystallographic characteristics. X‐ray diffraction analysis reveals a hexagonal crystal structure for NaY9Si6O26 ceramics within the P63/m (176) space group. Rietveld refinement analysis precisely determines the lattice constants as a = b = 9.3423 Å, c = 6.7524 Å, and a unit cell volume of V = 510.3877 Å3. Additionally, Raman spectroscopy unveils a noteworthy correlation between the quality factor and the full width at half maximum of the A1g(O) mode at 878 cm−1. The structural attributes, including lattice fringes and diffraction patterns of hexagonal NaY9Si6O26 ceramics, are elucidated through transmission electron microscopy. Of significance are the microwave dielectric properties of NaY9Si6O26 ceramics sintered at 1465°C, revealing a relative permittivity (εr) of 10.42, an impressive Q × f product of 33 766 GHz (at f = 11.14 GHz), and a temperature coefficient of resonant frequency (τf) of −28.7 ppm/°C. This comprehensive investigation contributes to the understanding of both the structural and microwave dielectric characteristics of NaY9Si6O26 ceramics, with potential applications in advanced electronic devices.
Water velocity shapes fish movement behavior
Authors: Hintz William D. , Porreca Anthony P. , Garvey James E. 
Published: January 2024

AbstractStream and river ecosystems present fluvial fishes with a dynamic energy landscape because moving water generates heterogeneous flow fields that are rarely static in space and time. Fish movement behavior should be consistent with conserving energy in these dynamic flowing environments, but little evidence supporting this hypothesis exists. Here, we tested experimentally whether three general movement behaviors—against the current, with the current, or holding position (i.e., staying in one position and location)—were performed in a way consistent with minimizing the cost of swimming in a heterogeneous flow field. We tested the effects of water velocity on movement behavior across three age classes (0, 1, and 5 years) of two different fluvial specialist fishes, the pallid sturgeon (Scaphirhynchus albus) and shovelnose sturgeon (Scaphirhynchus platorynchus). Individuals from the three age classes were exposed to a continuous and dynamic velocity field ranging from 0.02 to 0.53 m s−1, which represented natural benthic flow regimes occupied by these species in rivers. Both sturgeon species exhibited the same pattern with regard to their tendency to hold position, move upstream, or move downstream. Moving downstream was positively associated with velocity for all age groups. Moving upstream was inversely related to velocity for young fish, but as the fish aged, moving upstream was not related to water velocity. The oldest fish (age 5) moved upstream more frequently compared to the younger age classes. Holding position within a water current was the most frequent behavior and occurred with similar probability across the range of experimental velocity for youngest fish (age 0), but was inversely related to velocity in older fish. Our experiment across age classes suggests that the suite of swimming behaviors exhibited by fluvial specialists might have evolved to mitigate the energetic costs of complex energy landscapes generated by moving water to ultimately maximize net energy gain.
Histological differentiation between sporadic and colitis‐associated intestinal cancer in a nationwide study: A propensity‐score‐matched analysis
Authors: Uchino Motoi , Ikeuchi Hiroki , Noguchi Tatsuki , Okabayashi Koji , Futami Kitaro , Tanaka Shinji , Ohge Hiroki , Watanabe Kazuhiro , Itabashi Michio , Okamoto Kinya , Okita Yoshiki , Mizushima Tsunekazu , Mizuuchi Yusuke , Yamada Kazutaka , Shimada Yoshifumi , Sato Yu , Kimura Hideaki , Takahashi Kenichi , Hida Koya , Kinugasa Yusuke , Okuda Junji , Daito Koji , Koyama Fumikazu , Ueno Hideki , Yamamoto Takayuki , Hanai Tsunekazu , Kono Toru , Kobayashi Hirotoshi , Ajioka Yoichi , Sugihara Kenichi , Ishihara Soichiro ,   Study Group for Inflammatory Bowel Disease Associated Intestinal Cancers of the Japanese Society for Cancer of the Colon and Rectum
Published: January 2024

AbstractBackground and AimColitis‐associated intestinal cancer (CAC) can develop in patients with inflammatory bowel disease; however, the malignant grade of CAC may differ from that of sporadic colorectal cancer (CRC). Therefore, we compared histological findings distinct from cancer stage between CAC and sporadic CRC to evaluate the features of CAC.MethodsWe reviewed the clinical and histological data collected from a nationwide database in Japan between 1983 and 2020. Patient characteristics were compared to distinguish ulcerative colitis (UC), Crohn's disease (CD), and sporadic CRC. Comparisons were performed by using all collected data and propensity score‐matched data.ResultsA total of 1077 patients with UC‐CAC, 297 with CD‐CAC, and 136 927 with sporadic CRC were included. Although the prevalence of well or moderately differentiated adenocarcinoma (Tub1 and Tub2) decreased according to tumor progression for all diseases (P < 0.01), the prevalence of other histological findings, including signet ring cell carcinoma, mucinous carcinoma, poorly differentiated adenocarcinoma, or squamous cell carcinoma, was significantly higher in CAC than in sporadic CRC. Based on propensity score‐matched data for 982 patients with UC and 268 with CD, the prevalence of histological findings other than Tub1 and Tub2 was also significantly higher in those with CAC. At pT4, mucinous carcinoma occurred at a significantly higher rate in patients with CD (45/86 [52.3%]) than in those with sporadic CRC (13/88 [14.8%]) (P < 0.01).ConclusionCAC, including early‐stage CAC, has a higher malignant grade than sporadic CRC, and this difference increases in significance with tumor progression.
The effects of Jawoongo soap on skin improvement
Authors: Oh Sarang , Kim Hongyong , Kim Myeongju , Jin Xiangji , Zheng Shengdao , Yi Tae‐Hoo 
Published: January 2024

AbstractBackgroundJawoongo is used to treat and prevent skin issues such as dry and keratinization disorders, burns, trauma, pigmentation, scarring, and inflammatory skin conditions. In this study, the efficacy and safety of 0.47% Jawoongo extract‐containing soap (JAUN‐CS) were assessed in terms of skin improvement effects such as cleansing, moisturizing, sebum secretion management, and skin elasticity enhancement.MethodsTwenty healthy adult men and women aged 20–60 years old took part in the study. Before and after using JAUN‐CS, the participants were divided into groups, and various skin improvement effects were measured utilizing machines such as the Corneometer, Tewameter TM 300, and Visioscan. A dermatologist analyzed the product's safety in accordance with Frosch & Kligman and the Cosmetic, Toiletry, and Fragrance Association (CTFA) rules.ResultsUsing JAUN reduced the amount of base and point makeup by 25.7% and 76.7%, respectively. Also, JAUN showed a great facial exfoliation effect by removing the old and lifted skin keratins by 84.7% and 20.3%, respectively. Impurities in facial pores decreased by 58%, too. Furthermore, JAUN increased the moisture content of deep skin and skin surface by 3.5% and 74.0%, and skin elasticity by 2.8%. Skin tone, skin texture, skin radiance, and skin barrier all showed improvements of 3.3%, 20.0%, 15.0%, and 115.2%, respectively. Lastly, cleansing with JAUN successfully enhanced the condition of the youth triangle by 7.6%, while TEWL significantly decreased by 52.7%. Neither the JAUN nor the control group soap showed any adverse reactions, such as erythema or allergies, during the testing period.ConclusionsThe results of this study demonstrated that JAUN is safe for human use and has various skin‐improving properties, making Jawoongo a promising natural material for the development of functional cosmetics in the future.
Annual numbers of diagnoses and medical expenses for obstetric diseases in Japan: A report from the National Database of Health Insurance Claims
Authors: Nagata Chie , Suto Maiko , Morisaki Naho , Kobayashi Tohru , Takehara Kenji 
Published: January 2024

AbstractAimThe present study aimed to estimate the total numbers of obstetric diseases diagnosed, total amounts of medical expenses claimed for obstetric diseases, their averages per livebirth, and yearly trends in Japan.MethodsThis is a secondary analysis of the National Database of Health Insurance Claims and Specific Health Checkups of Japan (NDB) (data from 2015 to 2019). The target population was women of reproductive age (15–49 years old) with diseases in pregnancy, childbirth, and the puerperium, defined by having O codes according to the International Classification of Diseases 10th Revision. We calculated the numbers of obstetric diseases diagnosed, amounts of medical expenses claimed for obstetric diseases marked with the “main injury/disease decision flag,” and the totals divided by the annual numbers of livebirths, by year and women's age group.ResultsFrom 2015 to 2019, both the numbers of obstetric diseases diagnosed and amounts of medical expenses claimed for obstetric diseases per livebirth were on an upward trend, whereas the total numbers of obstetric diseases diagnosed were decreased. Women in advanced age groups had a higher number of diagnoses and a higher amount of medical expenses for obstetric diseases per livebirth. “Preterm labour without delivery” had the highest amounts of medical expenses claimed for and the second highest numbers of diagnoses throughout the study period.ConclusionsThis study suggests that pregnant women in Japan would have an increasing number of obstetric complications and necessary medical expenses year by year. Further study is warranted to elucidate these trends and identify possible mitigation measures.
Genetic structure of the commercially important Asian monsoon scallop, Amusium pleuronectes (Linnaeus 1758), across the Indonesian Archipelago
Authors: Hardianto Eko , Wijayanti Diah Permata , Imai Hideyuki 
Published: January 2024

AbstractThe Asian monsoon scallop, Amusium pleuronectes, is a key member of the most commercially harvested shellfish community in Asia. Patterns of genetic diversity and natural population structure in the target species were investigated to gain a better understanding of its evolutionary history. Samples were collected from five sites across the Indonesian Archipelago. We characterized sequence variation in an mtDNA control region fragment in 249 individuals. The genetic diversity (h = ranging from 0.83 to 0.92) and nucleotide diversity (π = ranging from 0.24 to 0.32) were low compared to the estimates reported for many other similar shellfish taxa. Nonetheless, analysis of molecular variance revealed significant genetic differentiation, FST = 0.0203 (p < .005 after Bonferroni corrections). Furthermore, both Pairwise fixation index values showed significance among majority population sites, indicating that dispersal potential and gene flow were low in the past. This pattern likely reflects a low dispersal potential, potentially allowing local adaptation to sites that augment any oceanographic and geographic contribution to genetic structure. The results described herein provide a foundation for developing better conservation strategies for the target species in the future.
Hormone and transcriptomic analysis revealed that ABA and BR are key factors in the formation of inter‐subgeneric hybridization barrier in water lily
Authors: Zhou Ping , Li Jingwen , Jiang Huiyan , Yang Zhijuan , Sun Chunqing , Wang Hongyan , Su Qun , Jin Qijiang , Wang Yanjie , Xu Yingchun 
Published: January 2024

AbstractUnderstanding the process of signal communication between pollen and stigma is of significant importance for plant sexual reproduction. In the case of inter‐subgeneric hybridization in water lily, a pre‐fertilization hybridization barrier exists, the regulatory mechanism of which remains unclear. In this study, we conducted hormone and transcriptome analyses of unpollinated stigmas (Mock), self‐pollinated stigmas (SP), cross‐pollinated stigmas within the same subgenus (CP), and inter‐subgenus cross‐pollination stigmas (ISCP) in water lily to elucidate the formation mechanism of the inter‐subgeneric hybridization barrier. Our results indicated that the lack of abscisic acid (ABA) and brassinolide (BR) in ISCP stigmas are key factors contributing to the formation of the inter‐subgeneric hybridization barrier in water lily. Exogenous application of ABA and BR can help overcome the barrier between inter‐subgeneric water lily crosses. Through transcriptome analysis, we identified nine candidate genes involved in regulating the inter‐subgeneric hybridization barrier in water lily. In addition, we further demonstrated the importance of the NCED2‐mediated ABA synthesis pathway in the pollination process through AS‐ODN technology. Our study confirms that ABA and BR are critical for breaking the inter‐subgeneric hybridization barrier. The identification of the nine candidate genes provides important clues for further research on the hybridization recognition mechanism in water lily.
The role of the epithelial sentinels, Langerhans cells and γδT cells, in oral squamous cell carcinoma
Authors: Hovav Avi‐Hai , Wilensky Asaf 
Published: January 2024

AbstractOral squamous cell carcinoma (OSCC) arises in the oral epithelium, a tissue in which immune surveillance is mediated by its primary resident leukocytes, Langerhans cells (LCs), and γδT cells. Under steady‐state conditions, LCs and γδT cells play a critical role in maintaining oral mucosal homeostasis. As antigen‐presenting cells of stratified epithelia, LCs respond to various challenges faced by the epithelium, orchestrating innate, and adaptive immune responses in order to resolve them. γδT cells also sense diverse epithelial insults and react rapidly through cytokine production and cytolytic activity. These epithelial sentinels are also considered to be the first leukocytes in the oral epithelium to encounter early carcinogenic events that have the potential of becoming OSCC. As evident in many malignancies, leukocyte populations help prevent cancer development although they also promote tumor progression. OSCC is no exception, as studies have reported both anti‐ and pro‐tumor roles of LCs and γδT cells. In this review, we summarize the ontogeny of LCs and γδT cells in the oral epithelium and discuss their role in OSCC.
Quantity over quality? How economic factors and welfare state interventions affected job insecurity and job quality before, during and after the economic crises
Authors: Antonucci Lorenza , Seo Hyojin , Strobl Martin 
Published: January 2024

AbstractThis article uses multilevel analysis of 24 European countries to examine the effects of macroeconomic variables (GDP and unemployment) and welfare state interventions (active and passive labour market policies) on job insecurity and job quality in Europe from the mid‐1990s until the last 2021 COVID crisis. The paper makes a distinction between the crisis of the welfare state and the reaction of welfare states to crises and connects the job quality literature with that on the transformation of the welfare state. The article introduces several innovations to the literature by looking at the impact of welfare state interventions on multidimensional job quality, distinguishing between different types of active labour market policy spending and considering the generosity of benefits. The findings show that active labour market policies (ALMPs) and passive labour market policies (PLMPs) have a positive effect in reducing job insecurity across skill groups. ALMPs and PLMPs also improved several dimensions of job quality, but mostly among manual/low‐skilled workers, while they have a negative effect on work pressure which mostly affects medium‐ and high‐skilled workers. The article concludes by discussing how, due to the reach of ALMP and PLMP interventions, the positive effects of the welfare state on job quality are concentrated among lower‐skilled workers, thereby limiting the ambition of contemporary welfare states to generate positive spillover effects on the quality of work for all workers.
Addressing the New Challenges for O&M of Floating Wind Turbine Systems
Authors: Leimeister Mareike , Baumgärtner David , Grotebrune Thilo , Hildebrandt Arndt , Kaczenski Jonas 
Published: January 2024

Abstract
Floating offshore wind turbines (FOWTs) are an essential technology for effectively exploiting the wind energy potential in deeper waters and could pave the way for rapid future growth of the offshore renewable energy market. Unlike the knowledge of accessibility and availability of bottom-fixed offshore wind farms, this information is relatively unknown for FOWTs and therefore subject to great uncertainty. Environmental conditions, type of transport vehicle, type of landing system or walk-to-work solutions, and floater geometry above and below the waterline are the main influencing factors for operation and maintenance (O&M) activities on FOWTs. Additionally, the human factor is more important in assessing the accessibility and maintainability of FOWTs. Knowledge, analysis, and modeling of relevant direct and relative motions of vessels, floaters, and their combined behavior are essential fundamentals for O&M. Finally, predicting the accessibility and availability of FOWTs is key to accurately assessing the developer’s and operator’s return on investment. Thus, this paper aims at providing guidelines on requirements for improving the maintainability and availability of FOWTs, including a proposed approach and potential toolchain. These recommendations are based on a case study investigating the different motion behaviors of vessels and FOWTs, and subsequently derived critical aspects for O&M activities on FOWTs.
FOWT Mooring System Design: Cost, Risk and Failure Implications
Authors: Weller Sam D. , Paterson Jack , MacIver Callum , Yeats Ben , Mackay Tom 
Published: January 2024

Abstract
Considering the design requirements of commercial-scale floating offshore wind turbine systems (FOWTs &gt; 10MW+) synergies exist with the existing offshore sector and it is unsurprising that conventional mooring and anchoring systems, and respective components are being proposed in the first instance. A key current industry discussion is focused on the subject of inherent redundancy and safety factors adopted for FOWT mooring systems. It is recognised that the risk profile for FOWT systems is different from that of oil and gas (O&G) installations and that existing O&G design codes may be overly conservative but to draw strong conclusions on this requires a robust analysis of cost and risk.
In order to provide meaningful insight into the implications of redundancy for a broad range of scenarios (e.g., mooring system types and configurations, sites and environmental conditions) exhaustive analyses to explore the mooring design parameter space is required. This paper outlines an efficient approach to identify a large number of potential mooring systems suitable for a commercial scale (15MW) FOWT as a precursor to carrying out lifecycle reliability and cost assessments.
Offshore Wind Feeder Vessel Voyage Simulations
Authors: Torres Rodriguez Adriana , Serraris Jorrit-Jan , Voogt Arjan 
Published: January 2024

Abstract
US cabotage laws and the low supply of Jones Act compliant Wind Turbine Installation Vessels (WTIV) necessitate the use of the feeder vessel system when installing offshore wind projects. This paper outlines a numerical methodology for determining weather impacts on installation operations and simulating the number of installation voyages that can be completed using a feeder vessel. One installation voyage consists of roundtrip transportation between the port and transfer of wind turbine generator (WTG) components at both ends. A representative 100.2 m vessel was used to demonstrate the applicability of this method. Ultimately, the results reflect strong dependence on seasonality, and as found in a previous study, the voyage is most sensitive to environmental conditions when transferring WTG components at the installation site [1, 2, 3]. Traveling between Northport, NY and the Bay State Wind lease area, the maximum amount of voyages achieved per month for this vessel was 10 due to configuration delays between the start and end of consecutive voyages. This figure increases to 14.1 per month when voyages occur consecutively. Finally, this method can be applied to other feeder vessels for individual analysis, comparison to each other, and even comparison against WTIVs to determine if adapting the feeder concept is advantageous in other countries where restrictions like the Jones Act are not a limiting factor.