bluuebunny/crossref_metadata_2025_split huggingface dataset:
ACACA reduces lipid accumulation through dual regulation of lipid metabolism and mitochondrial function via AMPK- PPARα- CPT1A axis
Authors: Dong Jian , Li Muzi , Peng Runsheng , Zhang Yuchuan , Qiao Zilin , Sun Na 
Published: December 2023

Abstract
Background
Non-alcoholic fatty liver disease (NAFLD) is a multifaceted metabolic disorder, whose global prevalence is rapidly increasing. Acetyl CoA carboxylases 1 (ACACA) is the key enzyme that controls the rate of fatty acid synthesis. Hence, it is crucial to investigate the function of ACACA in regulating lipid metabolism during the progress of NAFLD.
Methods
Firstly, a fatty liver mouse model was established by high-fat diet at 2nd, 12th, and 20th week, respectively. Then, transcriptome analysis was performed on liver samples to investigate the underlying mechanisms and identify the target gene of the occurrence and development of NAFLD. Afterwards, lipid accumulation cell model was induced by palmitic acid and oleic acid (PA ∶ OA molar ratio =1 ∶ 2). Next, we silenced the target gene ACACA using small interfering RNAs (siRNAs) or the CMS-121 inhibitor. Subsequently, experiments were performed comprehensively the effects of inhibiting ACACA on mitochondrial function and lipid metabolism, as well as on AMPK- PPARα- CPT1A pathway.
Results
This data indicated that the pathways significantly affected by high-fat diet include lipid metabolism and mitochondrial function. Then, we focus on the target gene ACACA. In addition, the in vitro results suggested that inhibiting of ACACA in vitro reduces intracellular lipid accumulation, specifically the content of TG and TC. Furthmore, ACACA ameliorated mitochondrial dysfunction and alleviate oxidative stress, including MMP complete, ATP and ROS production, as well as the expression of mitochondria respiratory chain complex (MRC) and AMPK proteins. Meanwhile, ACACA inhibition enhances lipid metabolism through activation of PPARα/CPT1A, leading to a decrease in intracellular lipid accumulation.
Conclusion
Targeting ACACA can reduce lipid accumulation by mediating the AMPK- PPARα- CPT1A pathway, which regulates lipid metabolism and alleviates mitochondrial dysfunction.
Post-transplant cyclophosphamide at 80mg/kg with low dose post-engraftment anti-thymocyte globulin in haploidentical transplantation with myeloablative conditioning
Authors: Hu Jiong , Wang Lining , XU Guilin , WANG Ling , Jiang Jieling , GAO Wenhui , Wan Ming , Blaise Didier 
Published: December 2023

Abstract
While post-transplant cyclophosphamide (PTCy) is commonly used as graft-versus-host disease (GvHD) prophylaxis in haploidentical stem cell transplantation (haplo-HSCT), the dose remains a matter of debate due to side effect concerns. Standard dose PTCy associated with tacrolimus and post-engraftment anti-thymocyte globulin (ATG) was used as the reference GvHD prophylaxis in our center and had demonstrated encouraging results. Though PTCy 80 mg/kg was shown to be feasible in patients in reduced intensity conditioning, whether it exerts equivalent GvHD prophylactic efficacy in myeloablative conditioning (MAC) setting has not been confirmed. Here, we retrospectively analyzed the efficacy and safety of PTCy 80mg/kg combined with tacrolimus and post-engraftment ATG as GvHD prophylaxis in patients aged more than 55 years or with cardiac antecedents or HCT-CI score > 2 undergoing haplo-HSCT with MAC. The cumulative incidence of grade III-IV aGvHD at day 100 and moderate-to-severe cGvHD at 1 year was 4.8%±3.4% and 19.9%±7.0%, respectively. When compared with patients receiving the reference regimen, patients from the PTCy 80mg/kg group had similar incidence of GvHDs and survival as their younger counterparts. Thus, PTCy 80 mg/kg seems to be feasible for patients treated with MAC conditioning regimens in haplo-HSCT, inviting further investigation notably in frail patients.
Shadow distributions: Deconstructing the geography of human impacts on species' natural distribution
Authors: Waldock Conor , Wegscheider Bernhard , Calegari Bárbara , Josi Dario , Brodersen Jakob , de Queiroz Luiz Jardim , Seehausen Ole 
Published: December 2023

Abstract

It remains unknown how species’ populations across their geographic range are constrained by multiple coincident natural and anthropogenic environmental gradients. Conservation actions are likely undermined without this knowledge because the relative importance of the multiple anthropogenic threats is not set within the context of the natural determinants of species’ distributions. We introduce the novel concept of a species “shadow distribution” to address this knowledge gap, using explainable artificial intelligence to deconstruct the environmental building blocks of species distributions. We assessed shadow distributions for multiple threatened freshwater fishes in Switzerland which indicated how and where species respond negatively to threats ̶ with negative threat impacts covering 87% of locations inside species’ ecological niches. Our findings indicate very widely used threat and biodiversity mapping exercises to be inadequate for the conservation of species geographic distributions. Overall, we show how priority actions for nature’s recovery can be identified and contextualised within the multiple natural constraints on biodiversity to better meet national and international biodiversity targets.
Life Cycle Capacity Evaluation for Battery Energy Storage Systems
Authors: Sun Yushu , Yue Xinyi , Zhuang Guofeng , Pan Yifan , Pei Wei , Tang Xisheng 
Published: December 2023

Abstract
Based on the SOH definition of relative capacity, a whole life cycle capacity analysis method for battery energy storage systems is proposed in this paper. Due to the ease of data acquisition and the ability to characterize the capacity characteristics of batteries, voltage is chosen as the research object. Firstly, the first-order low-pass filtering algorithm, wavelet packet decomposition algorithm, and empirical mode decomposition algorithm are used to decompose the voltage data of complete battery charging and discharging cycles into high & low frequency bands; Furthermore, the Pearson correlation coefficient is applied to analyze the linear relationship between high/low frequency band voltage and capacity, and to extract evaluation indicators related to capacity; Finally, the SOH evaluation accuracy of the proposed strategy through different application scenarios is verified to improve its generalization ability.
Nutritional scores predict the prognosis of patients with pulmonary tuberculosis
Authors: Tan Junyi , Tianju Li , Yinhuan Pi , Xiaofeng Shi , Mingque Xiang 
Published: December 2023

Abstract

Background: Although malnutrition is associated with poor prognosis in Pulmonary Tuberculosis (PTB) patients, there is currently no nutrition-based prediction model for PTB. Herein, we explored the applicability of common nutrition scores on the prognosis of PTB patients, aiming to construct a nutrition-based prediction model related to their prognosis.
Methods: We retrospectively collected clinical baseline data from 167 patients with secondary PTB who had not previously received anti-TB treatment. Subsequently, we determined the CONUT score, PNI index, and NPS score and evaluated the treatment efficacy using changes in lung lesions revealed by the chest CT scan. The Area Under the Receiver Operating Characteristic (AUROC) curve was used to quantify the predictive values of CONUT, PNI, and NPS scores for anti-TB efficacy in new-onset PTB patients, and the critical CONUT, PNI, and NPS values were determined using the Youden Index. We also performed univariate and multivariate analyses of prognostic factors in PTB patients to determine the nutrition scores and other clinical factors associated with the prognosis of patients with the new-onset PTB.
Results: The Youden Index revealed that the critical value of the CONUT score in PTB patients was 4.5, at which the sensitivity (72.2%) and specificity (96.6%) were highest. On the other hand, the critical cut-off values of the PNI index and the NPS score were 39.825 and 3.5, respectively. Univariate analysis of the predictors of poor prognosis in PTB patients showed that patients with diabetes, COPD, pneumonia, and hypoproteinemia (which were risk factors) had a poor prognosis (p<0.05). Multivariate analysis revealed that the CONUT score, PNI, NPS, and NRS-2002 were independent predictors of unfavourable PTB outcomes, with adjusted ORs of 0.016 (95%CI: 0.936-0.993, p<0.0001), 23.667 (95%CI: 9.317-60.115, p<0.0001), 0.119 (95%CI: 0.054-0.262, p<0.0001), and 0.124 (95%CI: 0.055-0.279, p<0.0001), respectively. The Area Under the Curve (AUC) of the CONUT score in predicting poor prognosis of PTB patients was 0.885 (95% CI：0.830-0.940，p<0.0001), which is comparable to that of the PNI index (0.862, 95% CI: 0.805-0.920, p<0.0001), but higher than that of NPS (0.774, 95% CI: 0.702-0.846, p<0.0001), BMI (0.627, 95% CI: 0.541-0.717, p<0.0001), and NRS-2002 (0.763, 95% CI: 0.688-0.838, p<0.0001). We discovered that older patients (p<0.0001), male participants (p< 0.05), and patients with Diabetes Mellitus (DM) (p<0.0001) and Chronic Obstructive Pulmonary Disease (COPD) (p<0.05) were more likely to have a high CONUT score.
Conclusion: The poor prognosis of PTB patients was related to a high CONUT score, low PNI index, and high NPS score, of which the specificity and sensitivity of the CONUT score were higher than those of the PNI index and the NPS score.
Utility of accessible SARS-CoV-2 specific immunoassays in vaccinated adults with a history of advanced HIV Infection
Authors: Ferrari Ludovica , Ruggiero Alessandra , Stefani Chiara , Benedetti Livia , Piermatteo Lorenzo , Andreassi Eleonora , Caldara Federica , Zace Drieda , Pagliari Matteo , Silberstein Francesca Ceccherini , Jones Christopher , Iannetta Marco , Geretti Anna Maria 
Published: December 2023

Abstract
Background. Accessible SARS-CoV-2-specific immunoassays may inform clinical management in people with HIV, particularly in case of persisting immunodysfunction. We prospectively studied their application in vaccine recipients with HIV, purposely including participants with a history of advanced HIV infection.
Methods. Participants received one (n=250), two (n=249) or three (n=42) doses of the BNT162b2 vaccine. Adverse events were documented through questionnaires. Sample collection occurred pre-vaccination and a median of 4 weeks post-second dose and 14 weeks post-third dose. Anti-spike and anti-nucleocapsid antibodies were measured with the Roche Elecsys chemiluminescence immunoassays. Neutralising activity was evaluated using the GenScript cPAss surrogate virus neutralisation test, following validation against a Plaque Reduction Neutralization Test. T-cell reactivity was assessed with the Roche SARS-CoV-2 IFNγ release assay.
Results. Primary vaccination (2 doses) was well tolerated and elicited measurable anti-spike antibodies in 202/206 (98.0%) participants. Anti-spike titres varied widely, influenced by previous SARS-CoV-2 exposure, ethnicity, intravenous drug use, CD4 counts and HIV viremia as independent predictors. A third vaccine dose significantly boosted anti-spike and neutralising responses, reducing variability. Anti-spike titres >15 U/mL correlated with neutralising activity in 136/144 paired samples (94.4%). Three participants with detectable anti-S antibodies did not develop neutralising responses post-third dose, yet displayed SARS-CoV-2 specific IFNγ responses.
Conclusions: SARS-CoV-2 vaccination is well-tolerated and immunogenic in adults with HIV, with responses improving post-third dose. Anti-spike antibodies serve as a reliable indicator of neutralising activity. Discordances between anti-spike and neutralising responses may be accompanied by detectable IFN-γ responses, underlining the complexity of the immune response in this population.
Community-based participatory research to guide adoption of culturally responsive trauma-informed HIV care throughout Nashville, Tennessee
Authors: Brown Lauren , Perkins Jessica , Acuña Jessica , Thacker Julie , Bolds Clare , Hawkins Mary , Stewart Jamie , Barroso Julie , Sommer Sadie , Eerden Joshua Van der , Heckman Bryan , Osman Amna , Smith Tarik , Alexander LaToya , Harvick Allie , Link Tiye , Crawley Anita , Nabaweesi Rosemary , Aboubaker Maria , Shaw-KaiKai Joanna , Foster Norman , Glaze-Johnson Beverly , Hoke Jessica , Audet Carolyn , Sales Jessica , Pettit April 
Published: December 2023

Abstract
Background: Psychological trauma is a highly prevalent driver of poor health among people with HIV (PWH) in the Southern United States (U.S.). Trauma-informed care (TIC) has potential to advance national Ending the HIV Epidemic (EHE) goals, but formative research is needed to tailor TIC implementation to complex and interdependent HIV networks. Methods: We applied a community-based participatory research (CBPR) approach to iteratively engage personnel from high volume HIV care institutions in Nashville, Tennessee. Current practices and potential implementation determinants were identified through participatory process mapping (PM) and key informant interviews. The Consolidated Framework for Implementation Research (CFIR) was applied to deductively code interview data. Personnel attending a dissemination summit developed a network-wide implementation plan.
Results: Data were collected with personnel from five institutions (e.g., community-based organizations, primary care clinics, public health department), for PM (n=48), interviews (n=35), and the summit (n=17). Results suggest there are limited trauma screenings, assessments, and services across the network. Relevant Characteristics of Individuals included a trauma-sensitive workforce committed to continuous learning and TIC adoption. Relevant Inner Setting Factors were networks and communications, with strong tension for change, high compatibility with TIC, and need for advancing cultural responsiveness. Relevant Outer Setting Factors included patient needs and resources and cosmopolitanism, with need for better leveraged mental health services. Relevant Process domains were champions and leadership, with need to diversify championship among leaders. Relevant Intervention Characteristics included relative advantage and complexity, with need for personnel wellness initiatives and increased engagement with the community as service designers. Four recommendations included development of shared communication systems, personnel wellness campaigns, routine evaluations to inform practices, and culturally responsive care initiatives.
Conclusion: Modifiable TIC determinants were identified, and a community-created implementation plan was developed to guide adoption. Future research will focus on city-wide implementation and strengthening pre-implementation research in other settings.
Alcohol consumption may be a risk factor for cerebrovascular stenosis
Authors: Liu Yiti , Gou Maoyuan , Gu Shuo , Guo Xiaoyan 
Published: December 2023

Abstract
Background: Atherosclerosis are well established risk factors for ischemic stroke, however the association between alcohol consumption and atherosclerosis is controversial. This study aims to explore the potential correlation between alcohol consumption and cerebral stenosis in patients with acute ischemic stroke.
Methods: Nine hundreds and forty-one patients with first acute ischemic stroke attack in 48 hours were recruited retrospectively. Alcohol consumption was classified into five consumption categories (non-drinkers, occasional drinkers, <140g per week, 140-279g per week, ≥280g per week). Computed tomography angiography (CTA) and digital subtraction angiography (DSA) were utilized to assess the carotid and cerebral artery in all patients. Five-step scale for degree of stenosis was applied: normal (0, 0 points), mild (<50%, 1 point), moderate (50% to 69%, 2 points), severe (70% to 99%, 3 points), and occlusion (100%, 4 points).
Results: The carotid and cerebral artery stenosis scores were positively correlated with moderate alcohol consumption (B = 1.466, P = 0.03). Compared with nondrinkers, moderate alcohol consumption had significant increasing risk of moderate carotid and cerebral artery stenosis (OR = 4.35, 95% CI:1.74-10.83, P = 0.008) and severe stenosis (OR = 3.26, 95% CI:1.35-7.89, P = 0.008) and occlusion (OR = 3.31, 95% CI:1.55-7.08, P = 0.002). Compared with nondrinkers, heavy alcohol consumption patients had significant higher risk of carotid and cerebral artery occlusion (OR = 2.96, 95% CI:1.43-6.12, P = 0.003).
Conclusions: Higher alcohol consumption may associate with higher risk and more severity of carotid andcerebrovascular stenosis.
Experimental study on mechanical properties of subgrade soil in cold region under different conditions
Authors: Wang Zecheng , Li Dongwei , Jia Zhiwen , Wang Zhenhua , Sun Qiao 
Published: December 2023

Abstract
In order to study the change of mechanical properties of subgrade under the condition of thawing after the highway in cold region is subjected to freeze-thaw action all the year round. In this paper, the effects of freeze-thaw cycles, freezing temperature, water content and confining pressure on the mechanical properties of subgrade soil in cold regions after thawing were studied by carrying out indoor mechanical tests, and the mechanical properties of subgrade soil in cold regions after thawing under different conditions were measured. The test results show that : 1 ) after 7 freeze-thaw cycles, the failure stress of subgrade soil decreases from 321.7 kPa to 289.9 kPa, with a decrease of 9.9%, and the elastic modulus decreases by 19.9%. 2 ) When the freezing temperature is reduced from − 5°C to − 15°C, the failure stress of subgrade soil is reduced from 303.9 kPa to 290.1 kPa, which is reduced by 13.8 kPa. The reduction range is about 4.5%, and the elastic modulus is about 1.6%;3 ) When the water content increased from 6–12%, the failure stress decreased from 405.43 kPa to 288.4 kPa, with a decrease of 29.1%, and the elastic modulus decreased approximately linearly, with a decrease of 50.4%. 4 ) When the confining pressure increases from 50 kPa to 150 kPa, the failure stress increases from 194.7 kPa to 367.7 kPa, and the failure stress increases by 88.8%. The elastic modulus increases by 154.1% with the increase of confining pressure. The research results can provide reference for highway and engineering construction in the western silty sand distribution area.
Genome-wide expression analysis of LACS gene family implies GHLACS25 functional responding to salt stress in cotton
Authors: Xu Yuchen , Fu Shouyang , Huang Yiwen , Zhou Dayun , Wu Yuzhen , Peng Jun , Kuang Meng 
Published: December 2023

Abstract
Background: Long-chain acyl-coenzyme A synthetase (LACS) is a type of acylating enzyme with AMP-binding, playing an important role in the growth, development, and stress response processes of plants.
Results: The research team identified different numbers of LACSgenes in four cotton species (Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii and Gossypium arboreum), indicating a certain diversity of LACS genes among different cotton species. By analyzing the structure and evolutionary characteristics of LACS genes, the GHLACS genes were divided into six subgroups, and a chromosome distribution map of the family members was drawn, providing a basis for further research classification and positioning. Promoter cis-acting element analysis showed that most GHLACSgenes contain plant hormones (GA, MeJA) or non-biological stress-related cis-elements, suggesting that GHLACS genes may be involved in the regulation of plant growth, development, and stress response processes. The expression patterns of GHLACS genes under salt stress treatment were analyzed, and the results showed that GHLACS genes may significantly participate in salt stress response through different mechanisms. The research team selected 12 GHLACS genes responsive to salt stress for tissue expression analysis and found that these genes are expressed in different tissues.
Conclusions: GHLACS25 was chosen for in-depth study, and it was confirmed that the GHLACS25 gene plays an important role in salt stress response through virus-induced gene silencing (VIGS) and induced expression in yeast cells. Overall, this study provides important clues and evidence for understanding the function of LACSgenes in cotton and their role in growth, development, and stress response processes. It is hoped that future research will further explore the mechanism of action of LACS genes in cotton and explore their potential application value in improving stress resistance traits.
Identification of FDA compounds to inhibit RAGEs through docking, MD-simulation, MMPBSA and DFT analysis
Authors: Singh Akash Pratap , Ahmad Shaban , Raza Khalid , Gautam Hemant K. 
Published: December 2023

Abstract
Receptor for advanced glycation end products (RAGEs) is a multifunctional cell surface protein present in vascular cells, monocytes/macrophages, B- and T-lymphocytes, retina Muller cells, kidney podocytes, mesangial cells, glial cells, neurons, and some cancer cells. The variety of cell types expresses RAGE, and AGEs ligand recognized by these receptor and produce signaling cascade mechanisms. The Accumulation of AGEs and recognisase by RAGEs play important roles in diabetes, chronic inflammatory disorders, neurological illnesses, and cancer, as well as T-lymphocyte proliferation. Therefore, block the RAGEs activity through FDA inhibitors are unmet need. In this studies, we targeted RAGE receptor and screening of FDA libraries through docking analysis. Docking studies identifed the best four FDA compounds Zytiga (ZINC000003797541), Paliperidone (ZINC000004214700), Targretin (ZINC000001539579), Irinotecan (ZINC000001612996) and Carboxymethyllysine (Control) were the medications examined; their respective ΔG values were -11.2, -11.2, -11.2, -11.1 and -5.0 kcal/mol respectively. Furthermore, 100 ns run was performed and found RMSD value of Paliperidone(1.2±0.3 nm) and Irinotecan (1.3± 0.2 nm) was quite stable. Next, docking and simulation experiment supported by MMPBSA analysis and found Paliperidone molecule was high negative energy (-13.49 kcal/mol). In silico investigation suggested that Paliperidone were involved in stable interactions which were maintained throughout the MD run with the RAGEs, virtually. The results advocated that these could potentially inhibit RAGEs activity and be used in the clinical management of various diseases.
Rosmarinic acid alleviates radiation-induced pulmonary fibrosis by downregulating tRNA N7-Methylguanosine modification-regulated fibroblast to myofibroblast transition through the exosomes pathway
Authors: Zhang Tingting , Mi Jinglin , Ouyang Zhechen , Qin Xinling , Wang Yiru , Li Zhixun , He Siyi , Hu Kai , Wang Rensheng , Huang Weimei 
Published: December 2023

Abstract
Background Radiation-induced pulmonary fibrosis (RIPF) is a common complication after radiotherapy in thoracic cancer patients, and there is a lack of effective treatment methods. The aim of this study was to explore the protective effect of rosmarinic acid (RA) on RIPF in mice as well as the underlying mechanism. Results We found that RA exerted an antifibrotic effect on lung tissues of RIPF mouse models and inhibited the progression of FMT through exosomes derived from lung epithelial cells. Mechanistically, RA reduced the transcription and translation efficiency of SPHK1 in lung fibroblasts by decreasing the tRNA N7-methylguanosine modification and downregulating the expression of tRNAs in lung epithelial cell-derived exosomes after irradiation, as well as inhibiting the interaction of SPHK1 with the NAT10 protein in fibroblasts. Furthermore, exosomes derived from irradiated lung epithelial cells after RA intervention decreased the acetylation and cytoplasmic translocation of PFKFB3, suppressing the FMT process triggered by glycolysis, and ultimately decelerating the progression of RIPF. Conclusions These findings suggest RA as a potential therapeutic agent for RIPF.