bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new hematological parameter model for the diagnosis and prognosis of sepsis in emergency department: A single‐center retrospective study
Authors: Zheng Xiaohe , Zhao Yating , Wang Dong , Pan Shiyao , Yushuaima  , Huang Zena , Ye Manman , Zhang Shihong 
Published: October 2023

AbstractIntroductionSepsis, a syndrome of organ dysfunction caused by an unregulated host response to infection. This study aimed to develop a novel sepsis diagnostic model of hematological parameters and evaluate its effectiveness in the early identification and prognosis of sepsis in emergency departments.MethodsA retrospective study was conducted in Emergency Department. Cell population data parameters related to monocytes and neutrophils were obtained using the Mindary BC‐6800 plus hematology analyzer. Receiver operating characteristic (ROC) curve analysis, logistic regression analysis was performed to assess the performance of the parameters and establish a diagnostic and prognostic model of sepsis, which was then verified with a validation cohort.ResultsMon_XW exhibited the best diagnostic performance (area under the ROC curve [AUC] = 0.848, 95% confidence interval [CI]: 0.810–0.885, p < 0.001), followed by Neu_Y and Neu_YW (AUC = 0.777 95% CI: 0.730–0.824, p < 0.001). Logistic regression analysis identified Mon_XW and Neu_Y as independent predictors, which were used to establish a diagnostic model named hematological parameter for sepsis (HPS). HPS demonstrated the best diagnostic performance with an AUC of 0.862 (95% CI: 0.826–0.898, p < 0.001), sensitivity of 70.0%, and specificity of 87.1%, compared to C‐reactive protein (CRP) and procalcitonin (PCT). The validation cohort also found that the positive predictive value of HPS was 70.4% and the negative predictive value was 92.2%.ConclusionThe developed HPS model showed promising diagnostic efficacy for sepsis in the emergency department, which outperformed CRP and PCT in terms of sensitivity and specificity. By enabling early identification and prognosis of sepsis, that contributes to reducing sepsis‐related mortality.
One‐step amplification refractory mutation system‐PCR/high‐resolution melting curve assay for carrier detection of red blood cell membranopathy caused by common SPTB mutations
Authors: Khongphithakskul Punnavit , Tangbubpha Noppawan , Khlangtan Tanyanee , Kadegasem Praguywan , Songdej Duantida , Sirachainan Nongnuch 
Published: October 2023

AbstractIntroductionHereditary pyropoikilocytosis (HPP) is the most common cause of non‐thalassemic severe inherited hemolytic anemia in Thai population. Up to 90% of affected patients harbor biallelic mutations of SPTB Providence (SPTB c.6055T>C), SPTB Buffalo (SPTB c.6074T>G), and SPTB Chiang Mai (SPTB c.6224A>G). This study aimed to develop a simple assay for mass screening of the three common SPTB mutations and to study their carrier frequencies in a healthy Thai population.MethodsWe combined multiplex amplification refractory mutation system‐PCR (ARMS‐PCR) and high‐resolution melting (HRM) curve analysis to create a one‐step single‐tube assay. The primers were designed to generate products with different melting temperatures in the presence of 6055C, 6074G, and 6224G. Internal control primers were added for quality control. Residual samples from blood donors and healthy adolescents were collected and tested for the three common SPTB mutations using the newly developed assay.ResultsOptimized multiplex ARMS‐PCR/HRM curve assay yielded well‐separated melt curves to detect the three SPTB mutations with 4‐h turnaround time. The assay was validated in screening of 2261 non‐repetitive blood donors and 89 adolescents, in which 10 (0.43%), 2 (0.09%), and 3 (0.13%) individuals were identified as carriers of SPTB Providence, SPTB Buffalo, and SPTB Chiang Mai, respectively. All mutated SPTB and 20 random wild‐type samples were confirmed using Sanger sequencing with 100% accuracy.ConclusionThe novel ARMS‐PCR/HRM curve assay is simple, accurate, and time‐effective for mass screening of the common SPTB mutations. This can be employed to prevent HPP birth in a Thai population.
Agentic interplay between hybridity and liminality in contemporary boundary work
Authors: Chamakiotis Petros , Symon Gillian , Whiting Rebecca 
Published: October 2023

AbstractThere is no doubt that digital technologies have an impact on boundary work, that is, the practices individuals develop to work up boundaries between ‘work’ and ‘life’. However, related research is still dominated by the integration–segmentation framework which may restrict our understanding of contemporary practice. To address this limitation, we use the concepts of hybridity (fusion) and liminality (in‐betweenness) in combination to explore how their interplay may promote more nuanced accounts of how individuals work up their boundaries in contemporary everyday life. Our analysis of video diaries and follow‐up interviews undertaken by 30 UK‐based workers reveals an ‘agentic interplay’ of hybridity and liminality that is understood as an ongoing dynamic practice, sometimes enabling and sometimes undermining desired boundary work outcomes. We make three contributions, collectively offering a critical advancement in the work–life debate and the hybridity and liminality literatures: (a) further developing an understanding of boundary work as situated, creative and dynamic; (b) unpacking the agentic potential of the ‘black box’ of the hybridity–liminality interplay; and (c) expanding current understandings and applications of the concepts of hybridity and liminality.
Laparoscopic vs. open surgery: A comparative analysis of wound infection rates and recovery outcomes
Authors: Shi Zhen 
Published: October 2023

AbstractAs medical technology continues to evolve, it becomes crucial to distinguish the outcomes of traditional versus modern surgical techniques. This was a retrospective cohort study, conducted to compare the wound infection rates and recovery outcomes between laparoscopic and open surgery (OS) in 180 patients. Participants were divided into laparoscopic (n = 105) and OS (n = 75) groups. Comprehensive data, including demographics, surgical details, and postoperative outcomes, were extracted. Wound infection rates, as measured by Wound Asepsis Score, were prioritized alongside recovery indicators such as duration of hospital stay and postoperative pain. The laparoscopic group had a mean operative time of 57.19 minutes, while the OS group required 85.10 ± 15.18 minutes (p < 0.05). Laparoscopic surgeries resulted in an average blood loss of 120.76 ± 13.3 mL compared to 170.01 ± 17.19 mL for OS (p < 0.05). The laparoscopic group had significantly reduced (p < 0.05) average hospital stays (2.1 ± 1.1 days) than the OS group (4.4 ± 2.1 days). Although the laparoscopic group had lower incidence of surgical site infection (4.76%) than the OS group (9.33%), this difference was not statistically significant (p > 0.05). On postoperative days 1, 3, and 7, the Visual Analog Scale assessment of pain in the laparoscopic group revealed significantly reduced pain (p < 0.05). Laparoscopic surgery appeared to offer distinct advantages over conventional open surgery, especially in terms of operative durations, recovery rates, and postoperative pain management. This study highlighted the potential of laparoscopic approaches for specific surgical interventions, as well as the importance of patient‐specific surgical strategy decision‐making.
Effects of burnout and work engagement in the relationship between self‐efficacy and safety behaviours—A chained mediation modelling analysis
Authors: Liu Man , Liu Lu , Lv Zhuoheng , Ma Fengyan , Mao Yousheng , Liu Yan 
Published: October 2023

AbstractAimsTo explore the current situation, influencing factors and pathways of safety behaviour of nurses in tumour specialized hospitals, in order to provide a theoretical basis for managers to manage and train nurses, improve their safety behaviour level and ensure medical safety.DesignAn anonymous cross‐sectional survey.MethodA total of 2147 nurses from Grade A cancer hospitals in 15 provinces of China were selected by a convenient sampling method. Questionnaires were collected through the Questionnaire Star platform. Nurses' safety behaviour was measured using the nurse Safety Behaviour Scale, Self‐efficacy by the General Self‐efficacy Scale, and nurses' occupational burnout was measured by the occupational Burnout Scale, and work engagement through the the Work Engagement Scale. Structural equation modelling was used to test the relationship among nurses' safety behaviour, general self‐efficacy, occupational burnout and work engagement. SPSS25.0 software was used to test the relationship among the safety behaviour of nurses, general self‐efficacy, occupational burnout and work engagement.ResultsThe total score of safety behaviour of nurses was 55.45 ± 6.879, the total score of general self‐efficacy was 31.39 ± 5.729, the total score of occupational burnout was 44.99 ± 26.587, and the total score of work engagement was 38.48 ± 13.433; the scores of the Nurse Safety Behaviour Scale, Self‐Efficacy Scale, and Work Engagement Scale were positively correlated (all p < .001); the occupational burnout scale was negatively correlated with the scores of self‐efficacy scale, work engagement scale and nurse safety behaviour scale (all p < .001); Structural equation model analysis shows that self‐efficacy and work engagement have a direct positive impact on nurse safety behaviour(β = .103, β = .096, all p < .001); Occupational burnout has a direct negative impact on self‐efficacy, work engagement and nurse safety behaviour(β = −.371, β = −.413, β = −.328 all p < .001). Bootstrap analysis showed that occupational burnout and job involvement had a significant chain mediating effect between self‐efficacy and the safety behaviour of nurses (95% CI: 0.148–0.21). The total effect of self‐efficacy on the safety behaviour of nurses was 0.283 (p < .001, 95% CI: 0.225–0.301), the direct effect was 0.096 (p < .001, 95% CI: 0.042–0.15), and the indirect effect was 0.179 (p < .001, 95% CI: 0.085–0.215), The mediating effect accounted for 63.3% of the total effect size.ConclusionOccupational burnout and work engagement play a partial mediating role between self‐efficacy and nurse safety behaviour. It is necessary to strengthen training on nurse safety culture awareness, improve the nurse self‐efficacy and work engagement, reduce nurse occupational burnout, and thereby improve the level of nurses' safety behaviour.
Comorbidity Between Reading Disability and Attention‐Deficit/Hyperactivity Disorder in a Community Sample: Implications for Academic, Social, and Neuropsychological Functioning
Authors: Willcutt Erik G. , Petrill Stephen A. 
Published: October 2023

AbstractTo better understand the implications of comorbidity between reading disability (RD) and attention‐deficit/hyperactivity disorder (ADHD), a sample of 225 participants with RD but without ADHD, 139 participants with both RD and ADHD, and 1,502 children without reading or attentional difficulties was recruited through five large public school districts. In comparison to the group without RD or ADHD, both groups with RD exhibited elevations of comorbid internalizing and externalizing disorders and significant global, academic, and social impairment. However, the group with both RD and ADHD was most impaired on most measures, and analyses of neuropsychological measures indicate that the co‐occurrence of RD and ADHD may be due at least in part to weaknesses in cognitive processing speed and working memory that are most severe in the comorbid group. These results indicate that psychoeducational assessments of RD should always screen for ADHD and other emotional and behavioral difficulties, and that when RD and ADHD co‐occur interventions are likely to be needed for both disorders.
Changes in dietary carbohydrate and lipid quality indices between 2008 and 2018: Analysis of the Brazilian dietary surveys
Authors: Cardoso Luciana Guerra , Monteiro Luana Silva , Sichieri Rosely , Yokoo Edna Massae , Pereira Rosângela Alves 
Published: October 2023

AbstractThe aim of the study was to evaluate characteristics and changes over a decade in dietary carbohydrate and lipid quality according to socio‐demographic variables. Data was obtained from two Brazilian National Dietary Surveys 2008–2009 (n = 34 003) and 2017–2018 (n = 46 164) examining a nationwide representative sample of individuals ≥10 years old. Food intake was assessed by means of two non‐consecutive diet records (2008–2009) and 24 h diet recalls (2017–2018). Carbohydrate Quality Index is a score ranging from 4 to 20 calculated from fibre intake, global dietary glycaemic index, solid/total carbohydrate (CHO) and whole grains/total grains CHO. Lipid Quality Index was estimated by dividing the sum of the dietary content of monounsaturated and polyunsaturated fatty acids by the sum of saturated and trans fatty acids. Indices were categorised into five categories (1 for lowest and 5 for highest quality). Socio‐demographic variables were sex, age, income, urban/rural area and place of food consumption. The estimates (95% CI) were generated separately for each survey and then compared to identify changes in time. Our main findings refer to changes in diet quality according to income. At the lowest income level, the proportions of individuals in the best carbohydrate and lipid quality categories reduced from 26.9% to 20.6% and from 30.0% to 24.9%, respectively. Alternatively, at the highest income level, these proportions increased from 22.9% to 26.6% and from 11.9% to 15.7%, respectively. Furthermore, the quality of lipids improved in women and among individuals reporting some away‐from‐home food consumption, while the quality of carbohydrates was reduced among adolescents and in rural areas.
The role of intensive care nurses in cellular treatments during the COVID‐19 pandemic
Authors: Caliskan Figen , Ozdemir Irem Nur , Zeydan Ayten , Kandemir Canan , Yilmaz Rabia , Karaoz Erdal , Adas Gokhan Tolga 
Published: October 2023

AbstractBackgroundToday, the use of cellular therapies as an effective treatment in the field of health is increasing. In the COVID‐19 pandemic or similar situations, cellular therapies may be sometimes life‐saving. The COVID‐19 pandemic has shown us that the training of intensive care nurses in special cases, such as cellular therapies, is insufficient.AimThe study aimed to determine the duties, responsibilities and training of intensive care nurses on mesenchymal stem cells (MSCs) transplantation to critically ill patients during the COVID‐19 pandemic.Study DesignThis descriptive and retrospective study was conducted on 107 critically ill patients diagnosed with COVID‐19 infection and followed up in the intensive care unit (ICU) between April 2020 and April 2022. Each patient was transplanted MSCs by intravenous infusion three times. Before starting cellular therapy applications, intensive care nurses were selected to work on this treatment modality. Each nurse was given theoretical and practical training by experienced instructors.ResultsIntensive care nurses trained for MSCs transplants took part in the pre‐application, preparation, application and post‐application period. MSCs were checked by the ICU nurses in the pre‐application period. Patients' vital signs, existing catheters, consciousness status and parameters were checked by nurses in the preparation and application period. No side effects and complications were observed in patients during MSCs transplantation and within the first 24 h. Patients' late complications and mortality were recorded by nurses during the post‐application periods.ConclusionsWe recommend that nurses working especially in Level 3 ICUs receive training and certification in cellular therapies, especially in hospitals where advanced/cellular treatments are applied.Relevance to Clinical PracticeIntensive care nurses are actively involved in every phase of the application of MSCs. Especially before such special practices, which came to the fore with the COVID‐19 pandemic, training should be organized for intensive care nurses.
Embryonic inhibition of colony‐stimulating factor 1 receptor induces enlarged cartilaginous zone of the midpalatal suture in postnatal mice
Authors: Yongzhen Lai , yan Guo , Jing Liu , Chenyan Ren , Chuanqing Mao , Yun Shi , Weihui Chen 
Published: October 2023

AbstractObjectivesThe midpalatal suture acts as the growth centre of the maxilla. Colony‐stimulating factor 1 receptor (CSF1R) is essential for osteoclastogenesis. Deletion of CSF1R, and its ligand, results in significant craniofacial phenotypes but has not been studied in detail in the midpalatal suture.Materials and MethodsPregnant ICR mice were treated with the CSF1R inhibitor PLX5622 at embryo Day 14.5 (E14.5) to E17.5. Pups at E18.5, postnatal Day 3 (P3) and P7 were collected for skeletal and histological staining. Osteoclasts were labelled using TRAP staining. PHH3 and TUNEL were employed to detect cell proliferation and apoptosis. Sox9, Ihh, and Col10a1 and Runx2, Col1a1, and DMP1 were used to detect chondrogenic differentiation and osteogenic differentiation, respectively. CD31, MMP9 and CTSK were utilized to assess vascular invasion and osteoclast secretion enzymes, respectively.ResultsEmbryonic inhibition of CSF1R resulted in a depletion of TRAP‐positive cells and an enlarged cartilage zone of the midpalatal suture of postnatal mice. Compared to those in the control group, Sox9, Ihh, Col10a1, Runx2 and Col1a1 were upregulated, whereas TUNEL and DMP1 were decreased in this zone. In the trabecular region, Col10a1 was upregulated, while TUNEL, Col1a1 and DMP1 were downregulated. Moreover, the expression of MMP9, CTSK and CD31 was decreased, and invasion into the cartilage zone was delayed.ConclusionsEmbryonic inhibition of CSF1R led to an abnormally enlarged cartilaginous zone in the midpalatal suture, potentially due to delayed endochondral ossification caused by the depletion of osteoclasts. Additionally, we established a novel model of midpalatal suture dysplasia, offering prospects for future research.
Conditioned pain modulation and psychological factors in young adults with recurrent or chronic neck pain
Authors: Arribas‐Romano Alberto , Fernández‐Carnero Josué , González‐Zamorano Yeray , Rodríguez‐Lagos Leonardo , Gurdiel‐Álvarez Francisco , Molina‐Álvarez Miguel , Morales Tejera David , Mercado Francisco 
Published: October 2023

AbstractBackgroundControversy exists with the presence of alterations in descending pain inhibition mechanisms in patients with non‐specific neck pain (NSNP). The aim of the present study was to evaluate the status of conditioned pain modulation CPM, remote pressure pain thresholds (PPT), and psychological factors in a specific subgroup of patients with NSNP such as young adult students. In addition, possible associations between CPM, psychological factors, and pain characteristics were analyzed.MethodsThirty students with recurrent or chronic NSNP and 30 pain‐free students were included in this cross‐sectional study. The following measures were assessed: CPM, remote PPT, psychological factors (depression, anxiety, pain catastrophizing, and kinesiophobia), pain characteristics (duration, intensity, severity of chronic pain, interference with daily life), and central sensitization inventory (CSI).ResultsNo significant differences were found in the efficacy of CPM between students with chronic or recurrent NSNP and pain‐free students (β coefficient = −0.67; 95% CI = −1.54, 0.20). However, students with pain showed a significantly higher remote PPT (mean difference = −1.94; 95% CI = −2.71, −1.18). and a greater presence of anxious (mean difference = 6; 95% CI = 2, 9) and depressive symptoms (mean difference = 8.57; 95% CI = 3.97, 13.16). In addition, significant moderate or strong correlations were found between CPM and pain intensity (partial r = 0.41), pain catastrophizing and mean pain intensity (r = 0.37), grade (r = 0.50), and interference of pain (r = 0.57), kinesiophobia and disability (r = 0.38), and depression and CSI (r = 0.39).ConclusionsYoung adult students with chronic or recurrent NSNP present remote hyperalgesia and symptoms of depression and anxiety but not dysfunctional CPM.
Prevalence of Chagas disease in Latin American pregnant women in Madrid, Spain: A multicentre cross‐sectional study from 2011 to 2016
Authors: Herrero‐Martínez Juan María , Trigo Elena , Navarro Miriam , García‐Alcázar Diana , Carrillo Irene , Fernández‐López Tania , Calderón‐Moreno María , Millán‐Pérez Rosario , Cuadros Juan , Velasco María , García‐Bujalance Silvia , Lizasoain Manuel , Martín‐Rabadán Pablo , Pérez‐Ayala Ana , Flores‐Chávez María ,   the Working Group on Chagas Disease of Madrid
Published: October 2023

AbstractObjectiveThe aim of this study is to assess Trypanosoma cruzi infection prevalence among pregnant migrants living in Madrid according to the country of origin and to assess screening coverage in this at‐risk population.MethodsRetrospective multicentre cross‐sectional study conducted from January 2011 to December 2016 in eight Madrid hospitals. Each hospital reviewed their microbiology data records to assess the screening coverage and serological diagnosis in all pregnant women coming from endemic areas.ResultsFrom 2011 to 2016, 149,470 deliveries were attended at the eight hospitals, and 11,048 pregnant women were screened for Chagas disease. Most cases (93.5%) were in women from Bolivia, who also showed the highest prevalence (12.4%, 95% confidence interval: 9.9–15.0). Pooled prevalence amongst the screened women was 2.9% (95% CI: 1.8–4.1). Chagas disease screening coverage varied greatly between centres, with a pooled mean coverage of 47% (95% CI: 37%–57%; 73% [95% CI: 63%–82%] for those centres with universal screening vs. 10% [95% CI: 6%–15%] for those with a selective screening approach; p < 0.001).ConclusionOur study provides useful data for policy makers and epidemiologists in a non‐endemic area without congenital Chagas screening programmes.
Integrin-dependent recruitment of Src to ROS-producing endosomes in osteoarthritic cartilage
Authors: Goldring Mary B. 
Published: October 2023

Fibronectin (FN) fragments stimulate catabolic signaling, and, by binding to integrins, they induce chondrocytes to increase the production of matrix metalloproteinases, including MMP-13. In this issue of
Science Signaling
, Miao
et al.
reveal that internalization of a FN fragment, but not intact FN, by α5β1 integrin results in the formation of ROS-producing endosomes (redoxosomes) through which chondrocytes detect and respond to damaged matrix by producing more MMP-13.
Near-complete genome sequence of
Lipomyces tetrasporous
NRRL Y-64009, an oleaginous yeast capable of growing on lignocellulosic hydrolysates
Authors: Jagtap Sujit Sadashiv , Liu Jing-Jing , Walukiewicz Hanna E. , Pangilinan Jasmyn , Lipzen Anna , Ahrendt Steven , Koriabine Maxim , Cobaugh Kelly , Salamov Asaf , Yoshinaga Yuko , Ng Vivian , Daum Chris , Grigoriev Igor V. , Slininger Patricia J. , Dien Bruce S. , Jin Yong-Su , Rao Christopher V. 
Published: October 2023

ABSTRACT

Lipomyces tetrasporous
is an oleaginous yeast that can utilize a variety of plant-based sugars. It accumulates lipids during growth on lignocellulosic biomass hydrolysates. We present the annotated genome sequence of
L. tetrasporous
NRRL Y-64009 to aid in its development as a platform organism for producing lipids and lipid-based bioproducts.