bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of the liver by multiple myeloma as nodular lesions: A case diagnosed by fine‐needle aspiration and immunocytochemistry
Authors: Fernandez‐Flores A. , Fortes J. , Smucler A. , Orduña M. , Pol A. 
Published: October 2003

AbstractLiver involvement by multiple myeloma as space‐occupying lesions is a rare condition with only a few cases reported in the literature. We present a case of a 68‐year‐old man with a prostatic adenocarcinoma who developed a multiple myeloma as a second primary malignancy. Hepatic nodules were discovered in the tomographic study. Fine‐needle aspiration of one of the hepatic lesions showed a pleomorphic plasmacytoid tumor. Immunocytochemistry using p63 and kappa‐chain antibodies was useful in determining the plasmacytic nature of the cells. Diagn. Cytopathol. 2003;29:280–282. © 2003 Wiley‐Liss, Inc.
Rhabdoid meningioma: Cytopathologic findings in cerebrospinal fluid
Authors: Parwani Anil V. , Mikolaenko Irina , Eberhart Charles G. , Burger Peter C. , Rosenthal Dorothy L. , Ali Syed Z. 
Published: October 2003

AbstractRhabdoid meningioma is a recently described, rare, WHO Grade III intracranial tumor with an aggressive growth pattern and increased risk of recurrence. We describe the cytopathologic findings on cerebrospinal fluid of one such case in a 26‐yr‐old female who underwent resection of a left temporo‐parietal mass. Cerebrospinal fluid contained abundant malignant cells with a prominent “rhabdoid” phenotype, i.e., large cells, eccentric nuclei, single prominent nucleoli, and dense eosinophilic cytoplasm. Although rhabdoid meningioma has a characteristic cytomorphology, the differential diagnosis of this tumor would involve metastatic adenocarcinoma, metastatic malignant melanoma, and other tumors with “rhabdoid” features (such as an atypical teratoid/rhabdoid tumor). Diagn. Cytopathol. 2003;29:297–299. © 2003 Wiley‐Liss, Inc.
Borderline nuclear change in cervical smears: Five‐year follow‐up
Authors: Grace A. , Maguire G. , Doherty S. , Thornhill M. , O'Dowd J. , Leader M. , Kay E. 
Published: October 2003

AbstractBorderline nuclear changes (BNC) account for 5–20% of cervical smears, and their outcome is unpredictable. Current management involves repeat smears at 6‐mo intervals, for a further 12 mo with referral for colposcopy, if the changes persist. Given the uncertainty surrounding the natural history of BNC and the claim that many patients are further investigated unnecessarily and potentially “overtreated,” the aim of this study was to determine the outcome for patients with a diagnosis of BNC to define whether the management protocol is appropriate for this patient group in our laboratory. A total of 808 patients with BNC were followed up. There was a 4.05% progression to high‐grade dyskaryosis, and most of these were detected within the first year of follow‐up. This at least justifies the early and intense follow‐up of this patient group until a reliable “triage tool” is adopted to pre‐select those patients with BNC who will progress or revert to negative. Diagn. Cytopathol. 2003;29:267–269. © 2003 Wiley‐Liss, Inc.
Metastatic lobular mammary carcinoma diagnosed in cervicovaginal smears: A case report
Authors: Rau Aarathi R. , Saldanha Prema , Raghuveer C. V. 
Published: October 2003

AbstractLobular carcinoma of the breast uncommonly metastasizes to the cervix. We report a case of lobular carcinoma of the breast with an abnormal cervicovaginal smear caused by metastasis in the cervix. We also discuss the differential diagnosis of primary genital and secondary adenocarcinomas in abnormal cervicovaginal smears. Diagn. Cytopathol. 2003;29:300–302. © 2003 Wiley‐Liss, Inc.
Parameter‐uniform numerical methods for a laminar jet problem
Authors: Ansari Ali R. , Hegarty Alan F. , Shishkin Grigori I. 
Published: October 2003

AbstractWe consider the classical problem of a two‐dimensional laminar jet of incompressible fluid flowing into a stationary medium of the same fluid. The equations of motion are the same as the boundary layer equations for flow past an infinite flat plate, but with different boundary conditions. Numerical experiments show that, using appropriate piecewise‐uniform meshes, numerical solutions together with their scaled discrete derivatives are obtained which are parameter (i.e., viscosity ν) robust with respect to both the number of mesh nodes and the number of iterations required for convergence. While the method employed is non‐conservative, we show with the aid of numerical experiments that the loss in conservation of momentum is minimal. Copyright © 2003 John Wiley & Sons, Ltd.
Highly efficient transgene‐independent recombination directed by a maternally derived SOX2CRE transgene
Authors: Vincent Stéphane D. , Robertson Elizabeth J. 
Published: October 2003

AbstractSummary: The Cre/loxP site‐specific recombination system is a powerful tool that allows gene inactivation in a tissue‐ and time‐specific manner. Several reports have shown that the Sox2Cre transgenic strain provides a very efficient means to delete gene function from the early epiblast (Hayashi et al.: Gene Expr Patterns 2:93–97, 2002; Vincent et al.: Genes Dev 17:1646–1662, 2003). Routinely, male studs carrying one null allele of the gene of interest and the Cre transgene are crossed to females homozygous for the conditional allele. Normally, excision is observed only in the progeny inheriting both the Cre transgene and the conditional allele. Here we report that when the Sox2Cre transgene is inherited maternally, excision occurs in all offspring irrespective of whether they carry the Cre transgene. Thus, Sox2Cre females provide a generally useful tool for rapid and efficient removal of loxP flanked sequences in vivo. genesis 37:54–56, 2003. © 2003 Wiley‐Liss, Inc.
Subtractive hybridisation screen identifies sexually dimorphic gene expression in the embryonic mouse gonad
Authors: McClive P.J. , Hurley T.M. , Sarraj M.A. , van den Bergen J.A. , Sinclair A.H. 
Published: October 2003

AbstractThe sex of most mammals is determined by the action of SRY. Its presence initiates testis formation resulting in male differentiation, its absence results in ovary formation and female differentiation. We have used suppression subtraction hybridisation between 12.0–12.5 days postcoitum (dpc) mouse testes and ovaries to identify genes that potentially lie within the Sry pathway. Normalised urogenital ridge libraries comprising 8,352 clones were differentially screened with subtracted probes. A total of 272 candidate cDNAs were tested for qualitative differential expression and localisation by whole mount in situ hybridisation; germ cell‐dependent or ‐independent expression was further resolved using busulfan. Fifty‐four genes were identified that showed higher expression in the testis than the ovary. One novel gene may be a candidate for interactions with WT1, based on its localisation to Sertoli cells and map position (16q24.3). genesis 37:84–90, 2003. © 2003 Wiley‐Liss, Inc.
Differential requirements for the neurogenic gene almondex during Drosophila melanogaster development
Authors: Michellod Marie‐Agnès , Forquignon Françoise , Santamaria Pedro , Randsholt Neel B. 
Published: October 2003

AbstractDuring early development, the neurogenic genes of Drosophila melanogaster are involved in the control of cell fates in the neurectoderm; almondex (amx) belongs to this category of genes. We have identified the amx locus and rescued the amx embryonic neurogenic phenotype with a 1.5 kb DNA fragment. Using a small deficiency, we generated a new amx mutant background called amxm, which is a null allele. Besides the characteristic neurogenic maternal effect caused by loss of amx, amxm flies display a new imaginal phenotype resembling loss of function of Notch. We describe amx‐induced misregulation of the Notch pathway target E(spl) m7 in embryos and genetic interactions between amx and Notch pathway mutants in adult flies. These data show that wildtype amx acts as a novel positive regulator of the Notch pathway and is required at different levels during development. genesis 37:113–122, 2003. © 2003 Wiley‐Liss, Inc.
Proximal upstream region of zebrafish bone morphogenetic protein 4 promoter directs heart expression of green fluorescent protein
Authors: Shentu Hsuan , Wen Hui‐Ju , Mour Her Guor , Huang Chang‐Jen , Wu Jen‐Leih , Hwang Sheng‐Ping L. 
Published: October 2003

AbstractWe examined the activity of the bone morphogenetic protein 4 (BMP4) promoter in zebrafish embryos via transient and stable transgenic expression analyses in order to obtain a better understanding of the regulation of BMP4 tissue‐specific expression. Transient expression studies showed that the 9.0‐kb BMP4 promoter/upstream region drove green fluorescent protein (GFP) expression mainly in the heart. Deletion analyses indicated the existence of multiple regulatory elements in the 7.5‐kb BMP4 promoter/proximal upstream region. In addition, a coinjection experiment further demonstrated the 2.4‐kb Bgl II–Hind III DNA region contains major positive regulatory elements. In addition, stable transgenic lines were established to further confirm the heart‐specificity of this segment in BMP4 promoter. The results showed that GFP was mainly localized in the myocardium of developing ventricles of 48‐hpf (hours postfertilization), 72‐hpf, and 100‐hpf transgenic F1 embryos. Together, these results indicate that the 7.5‐kb BMP4 promoter/proximal upstream region specifically contains regulatory elements for BMP4 expression in the heart, while regulatory elements for other endogenous BMP4‐expressing tissues may reside in more distal regions and/or in introns. genesis 37:103–112, 2003. © 2003 Wiley‐Liss, Inc.
Characterization of Notch3‐deficient mice: Normal embryonic development and absence of genetic interactions with a Notch1 mutation
Authors: Krebs Luke T. , Xue Yingzi , Norton Christine R. , Sundberg John P. , Beatus Paul , Lendahl Urban , Joutel Anne , Gridley Thomas 
Published: October 2003

AbstractThe Notch signaling pathway is an evolutionarily conserved signaling mechanism and mutations in its components disrupt cell fate specification and embryonic development in many organisms. To analyze the in vivo role of the Notch3 gene in mice, we created a deletion allele by gene targeting. Embryos homozygous for this mutation developed normally and homozygous mutant adults were viable and fertile. We also examined whether we could detect genetic interactions during early embryogenesis between the Notch3 mutation and a targeted mutation of the Notch1 gene. Double homozygous mutant embryos exhibited defects normally observed in Notch1‐deficient embryos, but we detected no obvious synergistic effects in the double mutants. These data demonstrate that the Notch3 gene is not essential for embryonic development or fertility in mice, and does not have a redundant function with the Notch1 gene during early embryogenesis. genesis 37:139–143, 2003. © 2003 Wiley‐Liss, Inc.
Naphthalene exposure: Effects on gene expression and proliferation in human cord blood cells
Authors: Diodovich Cristina , Malerba Ilaria , Bowe Gerard , Acquati Francesco , Bianchi Marco Giorgio , Taramelli Roberto , Parent‐Massin Dominique , Gribaldo Laura 
Published: October 2003

AbstractIn this study, the effect of naphthalene on the induction of apoptosis and gene expression profiles in cord blood cells has been evaluated, as well as its activity on the BCL‐2 related protein expression. After 6, 24, and 48 h of exposure to naphthalene (500 μM), a decrease in cell death was observed: the cells became more resistant to the toxicant and capable of surviving after the treatment. A Western blot analysis revealed an overexpression of BCL‐2, c‐JUN, c‐FOS, and RAF‐1 proteins, which are involved in the antiapoptotic response and in the regulation of cell growth, differentiation, and development. Furthermore, macroarray analysis showed that naphthalene modified cord blood gene expression, inducing IL‐8 precursor and T‐cell transcription factor and decreasing the level of RNA‐binding protein FUS/TLS. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:286–294, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10091
Effect of oxalomalate on lipid metabolism and antioxidant defense system in rats
Authors: Jung Un Ju , Kwon Oh‐Shin , Park Yong Bok , Huh Tae‐Lin , Lee Mi‐Kyung , Choi Myung‐Sook 
Published: October 2003

AbstractThe metabolic functions of NADP+‐specific isocitrate dehydrogenase (ID2), which may participate in the production of NADPH and biosynthesis of fatty acids, are not yet clearly understood. Accordingly, the current study investigated the effect of oxalomalate, known as a competitive inhibitor of ID2 in vitro, on lipid metabolism and the cellular defense system in vivo. Male Sprague Dawley rats (3 weeks old) were divided into two groups, fed a pelletized AIN‐76 semisynthetic diet for 8 weeks, and injected intraperioneally with either saline or oxalomalate (25 mg/kg BW) dissolved in saline every 2 days. Oxalomalate did not lower the body weight and adipose tissue weight significantly; however, it significantly lower the plasma leptin concentration (p < 0.000), plasma and hepatic triglyceride levels (p < 0.01, p < 0.05), and adipocyte lipoprotein lipase activity (p < 0.01) compared to the control group. Meanwhile, hepatic antioxidant enzyme activities, except for superoxide dismutase activity (p < 0.01), glutathione content, and thiobarbituric acid reactive substances levels were not significantly different between the groups. Therefore, the current data suggests that oxalomalate produces a triglyceride‐lowering activity and play a possible inhibitory role in fat accumulation. Furthermore, it was not found to affect the most antioxidative enzyme activities, glutathione content, and thiobarbituric acid reactive substances levels in rats fed normal diet. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:295–302, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10092
Primary linitis plastica carcinoma of the colon accompanied by peritoneal abscess
Authors: Fujii Yasutomo , Taniguchi Nobuyuki , Ono Tomoko , Omoto Kiyoka , Itoh Kouichi , Shitoh Kazuhisa , Okada Masaki , Yasuda Yoshikazu , Nagai Hideo 
Published: October 2003

AbstractWe describe the case of a 75‐year‐old woman with linitis plastica carcinoma of the colon, accompanied by a peritoneal abscess, in which the use of transabdominal sonography enabled prompt detection and diagnosis. Sonographic examinations revealed diffuse wall thickening with blurred layer stratification in the ascending colon. The irregular outer margin of the affected area was surrounded by thickened pericolic fat. A peritoneal abscess covered by the omentum was also found. CT confirmed these findings. We extensively resected the right half of the colon. Histopathologic examination of the excised segment of the colon revealed a poorly differentiated adenocarcinoma with fibrotic infiltration. The patient was discharged 6 weeks postoperatively, and chemotherapy was begun, but she was lost to our follow‐up. Although linitis plastica carcinoma of the colon is rare, it must be considered when patients have extensive colonic wall thickening with blurred layer stratification and an irregular outer margin surrounded by thickened pericolic fat. Transabdominal sonography should be considered the imaging modality of choice for the detection and diagnosis of this disease entity. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:488–492, 2003
Choledochal cysts in children: Evaluation with three‐dimensional sonography
Authors: Haliloglu Mithat , Akata Deniz , Gurel Safiye , Özmen Mustafa N. , Akhan Okan 
Published: October 2003

AbstractIn this report, we describe the application of 3‐dimensional (3D) sonography for diagnosing choledochal cysts in 3 girls who were 18 months, 2 years, and 11 years old. The 3D images that were obtained during the sonographic examination revealed that all 3 patients had a type I choledochal cyst and also demonstrated dilatation of the biliary tracts. The 3 patients then underwent surgical excision of their cysts, cholecystectomy, and hepaticojejunostomy. Besides its other clinical applications, 3D sonography promises to be a valuable adjunct to conventional 2‐dimensional imaging for the evaluation of choledochal cysts in pediatric patients. © 2003 Wiley Periodicals, Inc. J Clin Ultrasound 31:478–480, 2003
The nonisothermal decomposition kinetics of copper(II) complexes with phthalanilic acids and amino acids
Authors: Ren Li‐Ping , Dai Wei‐Lin , Cao Yong , Li He‐Xing , Zheng Xiao‐Hui , Fan Kangnian 
Published: October 2003

AbstractFifteen new kinds of mixed ligand complexes Cu(x‐P)A [P = 2‐(COO)C6H4CONH‐C6H4; x = H, 2‐Cl, 2‐CH3, 2‐OCH3, 4‐OCH2CH3; A = dehydronium Met, Leu, Phen] were synthesized. The thermal decomposition behavior of each complex is studied by TG. Coats‐‐Redfern, MacCallum‐‐Tammer, and Zsako methods are adopted to estimate the values of the apparent activation energy Ea, the activation entropy δS≠, the reaction order n, and the frequency factor A. The results showed that the reaction order is 2/3 for each of the complex. Studies on the mechanism of the thermal decomposition reactions suggested that these reactions all fit the kinetic equation 1 − (1 − α)1/3 = © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 623–628, 2003
Kinetic and thermodynamic study of the Na4(UO2)2(OH)4(C2O4)2 complex
Authors: Rodante Fabrizio , Vecchio Stefano , Materazzi Stefano , Vasca Ermanno 
Published: October 2003

AbstractThe synthesis, the characterization, and the thermal decomposition of the dioxouranium(VI) ternary complex of formula Na4(UO2)2(OH)4(C2O4)2, has been studied. The identification of the compound was performed by chemical analysis and by infrared spectrometry. Thermal decomposition of the compound occurs in several steps due to the decomposition of the salt to Na2O and UO3 oxides. The stoichiometry of the steps, hypothesized by means of the thermodynamic and kinetic parameters, is confirmed by the evolved gas analysis studied by FTIR spectrometer coupled to TG/DSC apparatus.Model‐fitting and model‐free kinetic methods have been used in kinetic analysis. The latter allows determining kinetic scheme. © 2003 Wiley Periodicals, Inc. Int J Chem Kinet 35: 661–669, 2003
Differences in vasculature between pilocytic and anaplastic astrocytomas of childhood
Authors: Gesundheit Benjamin , Klement Giannoula , Senger Christof , Kerbel Robert , Kieran Mark , Baruchel Sylvain , Becker Laurence 
Published: October 2003

AbstractBackgroundThe clinical manifestations of childhood pilocytic astrocytoma (PA) and anaplastic astrocytoma (AA) markedly differ, especially in the time to progression and prognosis. Because of the aggressive course and poor survival rate of AA, one would expect it to be associated with a high angiogenic index. Counterintuitively, we often find higher microvessel density counts in PA than in AA.ProcedureWe examined the differences in type or density of microvasculature between the two neoplasms. To differentiate established, mature vessels from immature growing ones, we used antibodies to Factor VIII (FVIII) to stain endothelial cells (ECs) of blood vessels and α‐smooth muscle actin (α‐SMA) antibodies to stain vessels supported by adventitia.ResultsWe found that large, mature, α‐SMA‐positive vessels predominated in PA, and small, immature, α‐SMA‐negative vessels in AA. The vessel maturation index was 54.5% for PA, and 6.1% for AA. Immunostaining with vascular endothelial growth factor (VEGF) and anti‐flt‐1/VEGF receptor‐1 antibodies showed distinct tissue patterns. VEGF immunoreactivity occurred mainly in the processes of the tumor astrocytes in PA; the opposite was observed in AA. flt‐1/VEGFR‐1 was detected in the tumor cells of AA but not in those of PA.ConclusionsWe propose that the predominance of small, α‐SMA‐negative vessels in AA represents immature, unstable vasculature with a potentially greater susceptibility to anti‐angiogenic therapy. The expression of both flt‐1 and VEGF by AA tumor cells also suggests a possible autocrine growth‐promoting function for VEGF in addition to its role as paracrine pro‐angiogenic growth factor for activated ECs, thus making anti‐angiogenesis an attractive therapeutic target in the treatment of AA. Med Pediatr Oncol 2003;41:516–526. © 2003 Wiley‐Liss, Inc.
Urodynamic evaluation of surgical outcome in patients with urinary retention due to central lumbar disc prolapse
Authors: Yamanishi Tomonori , Yasuda Kosaku , Yuki Takao , Sakakibara Ryuji , Uchiyama Tomoyuki , Kamai Takao , Tsujii Toshihiko , Yoshida Ken‐Ichiro 
Published: October 2003

AbstractAimsTo investigate whether acute urinary retention due to central lumbar disc prolapse is reversible after emergency spinal surgery.MethodsEight patients (two males, six females) with a mean age of 31.5 years (range, 18–42 years) with urinary retention due to lumbar disc prolapse were studied. An emergency surgery was performed within 48 hours (mean, 42 hours) after the onset of urinary retention. Urodynamic study was performed before and after the operation.ResultsAll patients had acontractile detrusor without bladder sensation and four of seven patients had inactive external sphincter on electromyogram at presentation. Two patients were unable to void up to 1 and 5 months after surgery and were then lost for follow‐up. The remaining six patients could void with straining or changing their voiding postures (bending forward), postoperatively. A follow‐up urodynamic study was performed in all patients from 1 month to 6 years postoperatively. All patients demonstrated acontractile detrusor on cystometrogram. On external sphincter electromyogram, four patients had normal activities. The remaining four patients recovered electromyographic activities, but two of them had denervation motor unit potentials and two had low activities.ConclusionsBladder function was irreversible after spinal surgery, whereas urethral function showed a better recovery in patients with acute urinary retention due to central lumbar disc prolapse. However, most of our patients could empty their bladder only by straining or changing their voiding postures postoperatively. Neurourol. Urodynam. 22:670–675, 2003. © 2003 Wiley‐Liss, Inc.