bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA‐365 Targets Nrf2 To Enhance Erastin-Induced Ferroptosis In Gastric Cancer Cells
Authors: Cao Hui , Ding Ming , Zhao Kai 
Published: October 2023

Abstract
Gastric cancer is globally recognized as a significant malignancy and a leading cause of cancer mortality. Ferroptosis, as a new form of regulated cell death, is attracting increasing attention in worldwide. As knows that, MicroRNA-365 (miR-365) has been implicated in the carcinogenesis of gastric cancer, but its role in ferroptosis remains elusive. The aim of our study is to clarify how miR-365 regulate ferroptosis in gastric cancer cells. Erastin is regarded as a ferroptosis inducer, our study found that with over-expression of miR-365 mimics also could enhance erastin-induced ferroptosis in gastric cancer cells. Additionally, miR-365 overexpression further upregulated the levels of MDA, Fe2+, and ROS in gastric cancer cells exposed to erastin, while antioxidant GSH levels were further downregulated, suggestive of enhanced lipid oxidation in gastric cancer cells upon erastin treatment through miR-365 overexpression. Notably, Nrf2 participated in the ferroptosis of cancer cells, as its upregulation was observed in gastric cancer cells after erastin exposure. qRT-PCR and Western blot analysis has shown that after exposed with erastin, the transcription and translation levels of Nrf2 in SGC-7901 and MGC-803 cells was increased. Overexpression of miR-365 in these cells attenuated the induction of Nrf2 by anti-aging drugs. The luciferase reporter assays data providing the evidence that miR-365 could directly target Nrf2. In conclusion, our study demonstrates that MicroRNA-365 could directly targets Nrf2. And its expression enhance ferroptosis induced by erastin in gastric cancer cells. This may provide a new target for therapy of gastric cancer regarding ferroptosis.
The effect of the pericardial patching exclusion technique in Left Atrial Appendage Closure on its postoperative reopening rate
Authors: wang Qinpu , Su Yijing , Zhong Jiajun , Li Jianan , Ren Xinlei , Xuan Junze , Huang Weicong , Wang Jue 
Published: October 2023

Abstract

Background: The left atrial appendage (LAA) is the primary source of cardiogenic embolism in atrial fibrillation patients. Traditional LAA closure methods have shown suboptimal clinical outcomes. In recent years, some surgeons have proposed employing a pericardial slice for suturing the LAA.
Methods: This study included patients with atrial fibrillation who underwent LAA closure during mitral valve surgery at our center between January 2019 and December 2021. There are two types of left atrial appendage closure surgical methods. The transesophageal echocardiogram performed 6 months after surgery will be used to verify the success of left atrial appendage closure. Left atrial appendage closure failure will be defined as reopening of the left atrial appendage or constant blood flow into the left atrial appendage.
Result: Among the 104 patients included (mean age: 65.0 ± 7.58 years, male: 52.50%), a total of 7 patients were found to have reopened left atrial appendage, of which 5 patients belonged to the suture closure group and 2 patients (15.6% vs 2.8%, p=0.047) belonged to the pericardial patch exclusion group.
Conclusions: The pericardial patch exclusion technique is a reliable technique to close the left atrial appendage in patients with atrial fibrillation, which can effectively reduce the possibility of reopening the left atrial appendage.
Permanent Left Bundle Branch Area Pacing Improves Mitral Regurgitation and Cardiac Function in Patients with Right Bundle Branch Block
Authors: Ma Jifang , Hu Juan , Song Weifeng , Chen Ke , Zang Xiaobiao , Zhou You , Wang Xianqing , Zhao Yonghui , Fu Haixia 
Published: October 2023

Abstract

Background
Permanent left bundle branch area pacing (LBBAP) can correct left bundle branch block. However, the correction of LBBAP in the right bundle branch block (RBBB) is undefined.
Objective
To investigate the effect and safety of permanent LBBAP in patients with RBBB.
Methods
Ninety-two consecutive patients who successfully experienced permanent LBBAP were recruited from May. 2019 to Dec. 2022 in our single center. Twenty patients with RBBB were analyzed in our study. They were followed up at 1, 3, 6 and 12 months after the LBBAP procedure. The QRS duration (QRSd) on the V1 lead of the 12-lead electrocardiogram was measured and compared before and after the LBBAP procedure, as well as left ventricular ejection (LVEF) and mitral regurgitation via echocardiography. The acute pitfills and delayed complications of the LBBAP procedure were recorded to assess safety.
Results
. The mean age of the RBBB patients was 66.35 ± 11.55 years, 60% were male, 75% had severe AVB, 20% had SSS, 25% had heart failure, 30% had atrial fibrillation, 45% had coronary heart diseases, 35% had hypertension, and 15% had diabetes mellitus. The average operation time of the LBBAP procedure was 106.53 ± 2.72 min, with 9 patients (45%) needing temporary cardiac pacing during surgery. QRS duration was significantly narrowed in RBBB patients after the LBBAP procedure (132.60 ± 31.49 vs. 119.55 ± 18.58 ms, P = 0.046). The average LVEF at the 12-month follow-up increased significantly from 55.15 ± 10.84% to 58.5 ± 10.55% (P = 0.018), with the median mitral regurgitation alleviated from 4.46 (0.9, 7.3) to 2.29 (0, 3.49) cm2 (P = 0.033). No ventricular septum perforation or pericardial effusion was found during the LBBAP procedure or follow-up.
Conclusion
LBBAP can shorten the QRS duration in patients with RBBB immediately, with mitral regurgitation and cardiac function improving during the 12-month follow-up.
Floquet stability analysis of a two-layer oscillatory flow near a flexible wall
Authors: Barcenas-Luque Antonio Jose , Coenen Wilfried , Gutiérrez-Montes Cándido , Martínez-Bazán Carlos 
Published: October 2023

Abstract

We investigate the linear Floquet stability of two fluid layers undergoing oscillations in the direction parallel to the flexible wall that separates them. This canonical configuration is inspired by the cerebrospinal fluid flow in the spinal canal of subjects with hydro-/syringomyelia. The analysis focuses on the marginal conditions for the onset of instability, and how these depend on the spatial wavelength of the perturbation, and on the values of the control parameters, which are the two channel widths, the Reynolds number, and the wall stiffness. Unstable perturbations are found to oscillate synchronous with the base flow. The wavelength of the most unstable perturbation, of the order of the stroke length of the basic oscillatory motion, depends strongly on the wall stiffness, but is only weakly influenced by the channel widths and the Reynolds number. In general, around criticality, it was found that increasing the Reynolds number has a destabilizing effect, and that decreasing the canal widths stabilizes the instability. The wall stiffness on the other hand has a non-monotonic effect, exhibiting an intermediate value for which the instability is maximally amplified. The present analysis is a first step towards a better understanding of the physical mechanisms that govern many (bio)fluid mechanical problems that involve oscillatory flows near compliant walls.
Maintenance immunosuppressive therapy in liver transplantation: results from CESIT study, an Italian retrospective cohort study
Authors: Bellini Arianna , Finocchietti Marco , Rosa Alessandro Cesare , Masiero Lucia , Trapani Silvia , Cardillo Massimo , Massari Marco , Alegiani Stefania Spila , Pierobon Silvia , Ferroni Eliana , Zanforlini Martina , Leoni Olivia , Ledda Stefano , Garau Donatella , Davoli Marina , Addis Antonio , Belleudi Valeria 
Published: October 2023

Abstract

Introduction
Post-liver transplantation immunosuppressive therapy typically involves the combination of various drugs: calcineurin inhibitors (tacrolimus-TAC or cyclosporine-CsA), with the potential addition of mycophenolate-MMF or mTOR inhibitors with/without corticosteroids. This study aimed to investigate the use of immunosuppressive treatments and compare their risk-benefit profiles in clinical practice.
Methods
This retrospective multicenter cohort study integrated data from the national transplant information system and administrative claims data from 4 Italian regions. All adult patients who underwent incident liver transplantation between 2009 and 2019 were identified and categorized into two groups: cirrhosis or hepatocellular carcinoma-HCC. The trend of immunosuppressive treatment over the years was analyzed, and their effectiveness/safety profiles were compared using multivariate Cox models (HR;95%CI).
Results
The study comprised 785 subjects in the cirrhosis cohort and 1,196 in the HCC cohort. Over the study years, there was a decline in the use of CsA, while combination therapy involving TAC with other drugs increased compared to monotherapy. Overall, TAC-monotherapy use was slightly over 40% in both groups, followed by TAC+MMF (39.5%-cirrhosis; 30.6%-HCC) and TAC+mTORi (8.5%-cirrhosis; 13.3%-HCC).
No significant differences emerged in risk-benefit profile of different TAC-based therapies, except for a higher risk of mortality in cirrhosis subjects under TAC-monotherapy compared to TAC+MMF (HR: 2.07;1.17-3.65).
Conclusions
The study highlights a shift over time in post-liver transplant therapeutic patterns, favoring the use of TAC in combination with MMF or mTORi, rather than monotherapy. Moreover, a potential association between TAC-monotherapy and increased mortality in the cirrhosis cohort was identified. Further research is warranted to optimize treatment strategies for liver transplant recipients.
Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses
Authors: DOĞAN Özgül , BUDAK Mahir , SALMAN Melissa Şafak , KORKMAZ Ertan Mahir 
Published: October 2023

Abstract
The complete genome of the European pine sawfly Neodiprion sertifer nucleopolyhedrovirus was sequenced and characterized from next-generation sequencing data of the N. sertifer larva from Turkiye. This genome was comparatively analysed by previously reported genomes baculoviruses. The baculovirus phylogeny was reconstructed and the species boundary of the NeseNPV-TR was delineated using K2P distance. The length of the genome was 82,052 bp, with a G + C content of 33.28%. It contained 82 putative ORFs, including 33 baculovirus core genes, three lepidopteran baculovirus core genes, three non-conserved genes. It had five hrs with 20.6% overall mean distance on average. The pairwise K2P distances of lef-9 and polh genes were lower than the specified threshold value, while those of lef-8, combinations of three genes and 33 genes were slightly higher between NeseNPV-TR and NeseNPV. The most variable genes were lef-2, helicase, p40, desmoplakin, p6.9, vp91 and vp39, while the most conserved were lef-8, lef-9, odv-e18, pif2 and lef-5 among baculoviruses. The genome of NeseNPV-TR is smaller and contains the fewest ORFs among baculoviruses. Some of unassigned ORFs had conserved domains and hence, we suggest further investigation to determine their structural and functional roles. Phylogenetic analyses confirmed its position within Gammabaculovirus. The NeseNPV-TR can be considered as the same species with NeseNPV. The different divergence rates in the baculovirus core genes may be related with different selection pressures acting on the genes. The lower genetic diversity of Group I alphabaculoviruses is most probably due to recent emergence.
Anopheles species associated with malaria transmission in gold-mining areas of northwest Colombia
Authors: Rojas Marco Andres , Velasco María Camila , Ahumada Martha , Chaparro Pablo , Yasnot Maria Fernanda , Orjuela Lorena Isabel 
Published: October 2023

Abstract

Background
In Colombia, mining is one of the main economic activities in four ecoepidemiological areas that contribute approximately 80% of malaria cases per year. Mining activity generates changes in the environment and opens new available breeding sites to be colonized by malaria vector mosquitoes in the area, increasing the malaria transmission risk.
Objective
To identify the presence of Anopheles species and their role in malaria transmission in five malaria-endemic localities with gold mining extraction in El Bagre, Antioquia, Colombia.
Methods
A cross-sectional study was conducted in five localities of El Bagre, Antioquia. Adult mosquitoes were collected indoors and outdoors. The taxonomic status of Anopheles species was confirmed by COI sequencing. All mosquitoes were tested by ELISA to establish infection with Plasmodium spp., and infections were confirmed using nested PCR. Immature mosquitoes were captured from the larval habitats. The association between the presence of Anopheles species and the physical parameters of larval habitat was estimated through multiple correspondence analysis and principal component analysis.
Findings:
Eleven Anopheles species were identified. Six specimens were positive for PCR Plasmodium test: An. darling infected with P. falciparum, An. nuneztovari s.s., and An. punctimacula infected with P. vivax. A total of 106 larval habitats were characterized. Using a multiple correspondence analysis, it was possible to associate the presence of An. triannulatus s.l., An. nuneztovari s.s., An. rangeli, An. oswaldoi B., An. albitarsis I, An. strodei, An. pseudopunctipennis and An. darlingi with larval habitats generated by mining activity.
Main conclusions:
An. darlingi, An. nuneztovari s.s. and An. punctimacula showed anthropophilic behavior. These species were detected infected with P. falciparum and P. vivax vk 210, confirming their role in malaria transmission in mining areas. The presence of immature stages of these species in gold mining excavations confirms these habitats as potential breeding sites for malaria vectors in El Bagre.
Development and Validation of a Protective Behavioral Strategies Scale for Individuals who use Opioids: Preliminary Findings and Future Directions
Authors: Luba Rachel , Gopaldas Manesh , Martinez Suky , Comer Sandra D. 
Published: October 2023

Abstract
Background: Protective Behavioral Strategies (PBS) are individually implemented harm reduction (HR) strategies to reduce the frequency or severity of risks associated with drug use. Existing scales measuring PBS for alcohol and cannabis suggest PBS are associated with reductions in associated problems. Despite many HR strategies related to opioid use, no PBS scale has been developed in the context of opioid use. To address this gap, this study aimed to test and validate a PBS scale for individuals using opioids (PBSO).
Methods: An online survey utilized a 32-item PBS scale for individuals endorsing recent opioid use, and measured opioid use frequency, HR service use, and experience of opioid overdose. PBSO items were rated on a Likert scale ranging from “never” (0) to “always” (6), and an exploratory factor analysis (EFA) examined factor structure.
Results: In the current sample (n=499; 32% female), EFA suggested a 3-factor structure among the 28 items retained, accounting for 51% of total variance. Factor 1 reflected health-service seeking, Factor 2 reflected individually-implemented and dose-reduction strategies, Factor 3 reflected social strategies, and Factor 4 reflected strategies related to injection drug use. Endorsement of PBSO items were slightly above “occasional” (3). PBSO use appeared positively related to past-month HR service utilization and negatively related to opioid use frequency.
Conclusions: Findings provide preliminary support for the PBSO scale as a valid and reliable measure. Further work is needed to test this scale in larger samples, and future work should explore the association between PBSO and relevant health outcomes, and whether factor scores differentially impact these outcomes.
Three-dimensional shear wave velocity models for the Atlantic upper mantle
Authors: James Esther K. , Komaravalli Davina S. 
Published: October 2023

Abstract

We investigate the three-dimensional shear-velocity structure of the upper mantle beneath the Atlantic Ocean using two approaches. One, we invert travel time measurements that were first presented in James et al., (2014) for approximately 10,000 fundamental-mode Rayleigh waves traversing the Atlantic basin, in the period range 30–125 seconds, to derive a layered 3-D model. Two, we explore continuous depth-dependent variations in vertically-polarized shear velocity, VSV, with a splined model by inverting phase velocities from the 2-D maps in James et al. (2014). For the layered model, we assume that VSV is constant in a given layer, which allows us to capture and compare the velocity structure in shallow and deep layers. Varying the boundary depth between the shallow and deep layers shows that age dependence is strongly observed in the shallow layers but has a very weak or no signal in the deep layers. This is the case even when the layer-separation boundary depth is as shallow as 100 km. For the splined models, we allow VSV to vary continuously with depth to observe gradual VSV changes with depth. Shear-velocity depth-profile comparisons reveal a significant reduction in age-dependence around 200-km depth. The splined-model approach also allows us to make quantitative shear velocity comparisons across several seafloor age regions and with results from previous studies of the Pacific basin (e.g., Nishimura and Forsyth 1989; Gaherty et al. 1996). We find that in some cases, shear velocities are higher in the Atlantic basin when compared to shear velocities in the Pacific basin for the same seafloor ages.
Gd-EOB-DTPA enhanced MRI is superior in selecting surgical candidates for patients with early-stage hepatocellular carcinoma: An analysis in terms of oncological outcomes
Authors: Ye Zhiwei , Zhao Jing , Hu Dandan , Yang Zhoutian , Chen Jinbin , Xu Li , Zhou Zhongguo , Chen Minshan , Zhang Yaojun 
Published: October 2023

Abstract
Purpose
To determine the value of preoperative Gd-EOB-DTPA-Enhanced Magnetic Resonance Imaging (MRI) vs the controls (extracellular contrast agent MRI and CT) in selecting surgical candidates for patients with hepatocellular carcinoma in terms of oncological outcomes after hepatectomy.
Methods
This retrospective study included 542 consecutive patients who underwent radical hepatectomy for HCC. One group underwent preoperative Gd-EOB-DTPA-Enhanced MRI and the other group underwent contrast enhanced CT or extracellular contrast agents MRI. We compared oncologic outcomes including recurrence free survival and overall survival between the three groups. Subgroup analyses were also performed to provide more specific candidates or beneficiaries for preoperative EOB-MRI.
Results
Totally 244 patients had tumor recurrence, with 55 in the EOB-MRI Group and 106 in the Routine-MRI Group and 83 in the CT Group (P = 0.010), as for early recurrence (< 2 years), 40 (27.03%) ,78(35.78%) and 62(35.22%) patients in each group (P = 0.018). The 1, 2, 3 years RFS were 82.4%, 73.0%, and 68.2% in EOB-MRI Group, 70.2%, 64.3%, and 56.9% in Routine-MRI Group, 76.8%, 64.83%, and 58.9% in CT Group(P = 0.010). The 1, 2, 3 years OS were 89.19%, 83.11%, and 80.41% in EOB-MRI Group, 79.82%, 73.86%, and 67.44% in Routine-MRI Group, and 86.55%, 76.14%, and 69.32% in CT Group (P = 0.016). Subgroup analysis showed that significant difference in RFS was observed in patients with solitary tumor < 3cm.
Conclusions
Preoperative EOB-MRI is superior to contrast-enhanced CT or extracellular contrast agents MRI in selecting surgical candidates at low risk of recurrence following hepatic resection for HCC.
Trust Development in Online Competitive Game Environments: A Network Analysis Approach
Authors: Fehlhaber Anna Lena , EL-Awad Usama 
Published: October 2023

Abstract
Trust seems to become established even in scenarios where the prerequisites for trust are complicated by conditions that evoke scepticism. Nonetheless, trust emerges, a phenomenon that is to be comprehended and examined in the present experimental inquiry. In order to comprehensively capture the process, a competitive online game environment was used to document the development of trust networks, directionality, and strength using network analysis. Despite the conditions conducive to distrust in this game setting, acts of trust were exhibited. Robust trust bonds persisting over the course of gameplay appear to manifest mostly dyadic or triadic, with participant embeddedness within the network and homophily in terms of general trustfulness towards strangers being conducive factors for trust bonding and game survivability. This study hence contributes to the overall understanding of online trust development and offers several further research opportunities in a mostly unexplored field.
Qualifying and Modeling Three Dimensions of Knowledge “mise en scène” in a College Space Tweeting Environment
Authors: Robert Abiodun Charles 
Published: October 2023

Abstract
The work evaluated evolving economy as heavily characterized by the knowledge of the untapped college students and their activities that is external to four walls of classical learning. Such knowledge was deemed social knowledge that is qualifiable and exchangeable. Characteristically, such social knowledge was seen from three factors of personalization, environment, and social interactions. It was given a digital dimension that can be expressed as JSON object. Scenario of usage was attempted at the end of the discussion. The overall objective was to prepare the ground for evaluating knowledge of college students outside the conventional factors presently in use. This was conceived particularly possible when applied to technology of social media.
The demographic, laboratory and genetic factors associated with Long Covid-19 Syndrome: a case-control study
Authors: Torki Ensiye , Hoseininasab Fahimeh , Moradi Marjan , Sami Ramin , Sullman Mark J. M. , Fouladseresht Hamed 
Published: October 2023

Abstract
Objective
Long Covid-19 syndrome (LCS) manifests with a wide range of clinical symptoms, yet the factors associated with LCS remain poorly understood. The current study aimed to investigate the relationships that demographic characteristics, clinical history, laboratory indicators, and the frequency of HLA-I alleles have with the likelihood of developing LCS.
Methods
We extracted the demographic characteristics and clinical histories from the medical records of 88 LCS cases (LCS+ group) and 96 age/sex-matched individuals without LCS (LCS− group). Furthermore, we evaluated the clinical symptoms, serum levels of interleukin (IL)-6 and tumor necrosis factor-α, laboratory parameters, and the frequencies of HLA-I alleles. Following this we used multiple logistic regression to investigate the association these variables had with LCS.
Results
Subjects in the LCS+ group were more likely to have experienced severe Covid-19 symptoms and had higher body mass index (BMI), white blood cell, lymphocyte counts, C-reactive protein (CRP), and IL-6 levels than those in the LCS− group (for all: P˂0.05). Moreover, the frequencies of the HLA-A*11, -B*14, -B*38, -B*50, and -C*07 alleles were higher in the LCS+ group (for all: P˂0.05). After adjusting for the most important variables, the likelihood of suffering from LCS was significantly associated with BMI, CRP, IL-6, the HLA-A*11, and -C*07 alleles, as well as a positive history of severe Covid-19 (for all: P˂0.05).
Conclusion
Our study showed that a history of severe Covid-19 during the acute phase of the disease, the HLA-A*11, and -C*07 alleles, higher BMI, as well as elevated serum CRP and IL-6 levels, were all associated with an increased likelihood of LCS.