bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbonic anhydrase inhibitors limit complications in X-linked retinoschisis
Authors: Wey Stephanie , Brill Daniel A. , Miraldi Utz Virginia , Sisk Robert A. 
Published: November 2023

PurposeCarbonic anhydrase inhibitors (CAIs) reduce macular schisis in patients with X-linked retinoschisis (XLRS). The purpose of this study was to determine if CAIs reduce the incidence of complications from XLRS, including macular atrophy, retinal tears, and retinal detachment (RD), the most common causes of vision loss in patients with XLRS.MethodsFor this retrospective interventional case series, a chart review of patients examined at Cincinnati Children’s Hospital Medical Center [CCHMC] and Cincinnati Eye Institute [CEI] between 1/1/2015 and 1/16/2023 was performed. Male patients were included based on genetically-confirmed RS1 or typical clinical presentation with known family history of XLRS with at least two follow-up visits.ResultsTwenty-eight patients (56 eyes) with XLRS were included. There were 10 RS1 variants among the 21 genotyped patients. Median age at clinical diagnosis was 10.4 years old (range: 0.4–55.7 years) with median follow-up time of 4.7 years (range: 0.2–38.3 years). Median presenting Snellen visual acuity was 20/60 (logMAR 0.48, range: 0.18–3). In 26 eyes of 15 patients treated with CAIs, median CST pre-treatment was 416 microns (range: 198–701 microns), and median percentage decrease in CST on treatment was 21.8% (range: 0–74.5%) from highest pre-treatment CST. Reduction in CST with CAI use was statistically significant (p = 0.02), but not logMAR VA (p = 0.64). There was no significant difference in CST between patients treated with topical vs. oral CAI (p = 0.95) or between patients with partial or complete CAI adherence (p = 0.60). Ten eyes of seven patients had an RD requiring surgical intervention. No treated eyes developed new macular atrophy, peripheral retinoschisis, retinal tears, or RD; two eyes on topical CAIs had spontaneous resolution of bullous peripheral retinoschisis.ConclusionDuring the follow-up period, patients taking CAIs reduced macular schisis and did not experience new complications of macular atrophy, retinal tears, or RD. This is a relatively large cohort with long-term follow-up periods for patients with XLRS. Reduced macular schisis may not require perfect adherence with CAIs. A large, prospective, randomized, controlled clinical trial is needed to determine the potential of CAIs to improve visual function, reduce retinoschisis, and prevent RD.
Microbiota of maize kernels as influenced by Aspergillus flavus infection in susceptible and resistant inbreds
Authors: Moore Geromy G. , Chalivendra Subbaiah , Mack Brian M. , Gilbert Matthew K. , Cary Jeffrey W. , Rajasekaran Kanniah 
Published: November 2023

BackgroundNearly everything on Earth harbors a microbiome. A microbiome is a community of microbes (bacteria, fungi, and viruses) with potential to form complex networks that involve mutualistic and antagonistic interactions. Resident microbiota on/in an organism are determined by the external environment, both biotic and abiotic, and the intrinsic adaptability of each organism. Although the maize microbiome has been characterized, community changes that result from the application of fungal biocontrol strains, such as non-aflatoxigenic Aspergillus flavus, have not.MethodsWe silk channel inoculated field-grown maize separately with a non-aflatoxigenic biocontrol strain (K49), a highly toxigenic strain (Tox4), and a combination of both A. flavus strains. Two maize inbreds were treated, A. flavus-susceptible B73 and A. flavus-resistant CML322. We then assessed the impacts of A. flavus introduction on the epibiota and endobiota of their maize kernels.ResultsWe found that the native microbial communities were significantly affected, irrespective of genotype or sampled tissue. Overall, bacteriomes exhibited greater diversity of genera than mycobiomes. The abundance of certain genera was unchanged by treatment, including genera of bacteria (e.g., Enterobacter, Pantoea) and fungi (e.g., Sarocladium, Meyerozyma) that are known to be beneficial, antagonistic, or both on plant growth and health.ConclusionBeneficial microbes like Sarocladium that responded well to A. flavus biocontrol strains are expected to enhance biocontrol efficacy, while also displacing/antagonizing harmful microbes.
Proportions of four distinct classes of sensory neurons are retained even when axon regeneration is enhanced following peripheral nerve injury
Authors: Khan Samia , Carrasco Dario I. , Isaacson Robin , English Arthur W. 
Published: November 2023

IntroductionRecovery from peripheral nerve injuries is poor because axon regeneration is slow and inefficient. Experimental therapies that increase signaling of neuronal brain-derived neurotrophic factor (BDNF) through its TrkB receptor or through its downstream effectors enhance axon regeneration, increasing the number of motor and sensory neurons whose axons successfully regenerate and reinnervate muscle targets. The goal of this study was to compare the proportions of four different classes of sensory (dorsal root ganglion, DRG) neurons that successfully reinnervate two different muscle targets in control mice and mice treated pharmacologically to enhance axon regeneration.MethodsFollowing sciatic nerve transection and repair, C57BL/6 J mice were treated for 2 weeks, either with R13, a prodrug that releases the small molecule TrkB ligand, 7,8-dihydroxyflavone, with compound 11 (CP11), an inhibitor of asparaginyl endopeptidase (δ-secretase), or with a control vehicle. Four weeks after injury, different fluorescent retrograde tracers were injected into the gastrocnemius and tibialis anterior muscles to mark DRG neurons that had successfully reinnervated these muscles. Using immunofluorescence, retrogradely labeled DRG neurons also expressing markers of four different sensory neuronal classes were counted.Results and discussionTreatments with R13 or CP11 resulted in muscle reinnervation by many more DRG neurons than vehicletreated controls, but neurons expressing proteins associated with the different classes of DRG neurons studied were largely in the same proportions found in intact mice.
Peak systolic velocity ratio for evaluation of internal carotid artery stenosis correlated with plaque morphology: substudy results of the ANTIQUE study
Authors: Pakizer David , Vybíralová Anna , Jonszta Tomáš , Roubec Martin , Král Michal , Chovanec Vendelín , Herzig Roman , Heryán Tomáš , Školoudík David 
Published: November 2023

BackgroundAccurate assessment of carotid stenosis severity is important for proper patient management. The present study aimed to compare the evaluation of carotid stenosis severity using four duplex sonography (DUS) measurements, including peak systolic velocity (PSV), PSV ratio in stenosis and distal to stenosis (PSVICA/ICA ratio), end-diastolic velocity (EDV), and B-mode, with computed tomography angiography (CTA), and to evaluate the impact of plaque morphology on correlation between DUS and CTA.MethodsConsecutive patients with carotid stenosis of ≥40% examined using DUS and CTA were included. Plaque morphology was also determined using magnetic resonance imaging. Spearman’s correlation and Kendall’s rank correlation were used to evaluate the results.ResultsA total of 143 cases of internal carotid artery stenosis of ≥40% based on DUS were analyzed. The PSVICA/ICA ratio showed the highest correlation [Spearman’s correlation r = 0.576) with CTA, followed by PSV (r = 0.526), B-mode measurement (r = 0.482), and EDV (r = 0.441; p &lt; 0.001 in all cases]. The worst correlation was found for PSV when the plaque was calcified (r = 0.238), whereas EDV showed a higher correlation (r = 0.523). Correlations of B-mode measurement were superior for plaques with smooth surface (r = 0.677), while the PSVICA/ICA ratio showed the highest correlation in stenoses with irregular (r = 0.373) or ulcerated (r = 0.382) surfaces, as well as lipid (r = 0.406), fibrous (r = 0.461), and mixed (r = 0.403; p &lt; 0.01 in all cases) plaques. Nevertheless, differences between the mentioned correlations were not statistically significant (p &gt; 0.05 in all cases).ConclusionPSV, PSVICA/ICA ratio, EDV, and B-mode measurements showed comparable correlations with CTA in evaluation of carotid artery stenosis based on their correlation with CTA results. Heavy calcifications and plaque surface irregularity or ulceration negatively influenced the measurement accuracy.
Disease activity in primary progressive multiple sclerosis: a systematic review and meta-analysis
Authors: Blok Katelijn M. , van Rosmalen Joost , Tebayna Nura , Smolders Joost , Wokke Beatrijs , de Beukelaar Janet 
Published: November 2023

BackgroundDisease activity in multiple sclerosis (MS) is defined as presence of relapses, gadolinium enhancing lesions and/or new or enlarging lesions on MRI. It is associated with efficacy of immunomodulating therapies (IMTs) in primary progressive MS (PPMS). However, a thorough review on disease activity in PPMS is lacking. In relapsing remitting MS, the prevalence of activity decreases in more contemporary cohorts. For PPMS, this is unknown.AimTo review disease activity in PPMS cohorts and identify its predictors.MethodsA systematic search in EMBASE, MEDLINE, Web of science Core Collection, COCHRANE CENTRAL register of trials, and GOOGLE SCHOLAR was performed. Keywords included PPMS, inflammation, and synonyms. We included original studies with predefined available data, extracted cohort characteristics and disease activity outcomes and performed meta-regression analyses.ResultsWe included 34 articles describing 7,109 people with PPMS (pwPPMS). The weighted estimated proportion of pwPPMS with overall disease activity was 26.8% (95% CI 20.6–34.0%). A lower age at inclusion predicted higher disease activity (OR 0.91, p = 0.031). Radiological activity (31.9%) was more frequent than relapses (9.2%), and was predicted by longer follow-up duration (OR 1.27, p = 0.033). Year of publication was not correlated with disease activity.ConclusionInflammatory disease activity is common in PPMS and has remained stable over the last decades. Age and follow-up duration predict disease activity, advocating prolonged monitoring of young pwPPMS to evaluate potential IMT benefits.
Effect of orthopedic insoles on spinal deformity and walking in adolescents with idiopathic scoliosis summary
Authors: Li Yangzheng , Xiaoli Huang , Ye Nan , Songjian Xin , Li Liu , Qianqi Huang , Yining Yan , Li Changsheng 
Published: November 2023

ObjectiveTo observe the effects of scoliosis-specific exercise therapy combined with braces and orthopedic insoles on improved spinal deformity and walking ability in adolescents with idiopathic scoliosis (AIS).MethodFrom September 2019 to September 2020, 60 outpatient AIS patients were distributed into brace group (n = 30) at random and brace combined orthopedic insole group (n = 30). Both groups underwent brace dryness, and the observation group used scoliosis-specific exercise therapy combined with brace therapy, and on this basis, orthopedic insole intervention was added for 8 h per day for 2 months. At the same time, 20 adolescents of the same age with normal spinal development were recruited as a healthy group. GaitScan instruments were used to collect gait and plantar pressure measurements from study subjects. First, the gait and plantar pressure data of AIS patients and healthy groups were compared horizontally to ascertain the abnormal indicators, and then the spinal deformity and the above abnormal indicators were compared between the brace group and the brace combined orthopedic insole group.OutcomeThe plantar pressure center drift index (CPEI) in the AIS group was higher than that in the healthy group (F = 3.120, P &lt; 0.05), and there were significant differences in the ratio of medial and lateral heel pressure (M/l) and total foot pressure (P &lt; 0.05) between the AIS group and the healthy group, and no noticeable variations were found in the support phase period, walking speed, and proportion of each phase (P &gt; 0.05). After treatment, the Cobb angle was significantly reduced in both the brace group and the brace combined with orthopedic insole group (P &lt; 0.05), and there was no significant difference between the groups (P &gt; 0.05). There were no significant changes in the pressure ratio of CPEI, M/l and bilateral full foot in the brace group (P &gt; 0.05). The CPEI decreased in the brace combined with orthopedic insole group (P &lt; 0.05), and the pressure ratio of M/l and bilateral full foot tended to 1 (P &lt; 0.05), and was better than that in the brace group (P &lt; 0.05).ConclusionPatients with AIS may have local and worldwide asymmetric changes in plantar pressure distribution. The addition of orthopedic insoles has limited effect on improving scoliosis deformity in patients with AIS, but it can effectively improve the abnormal biomechanics of patients with AIS, so that the patient's force tends to be balanced.
Non-linear relationship between sleep duration and blood pressure in children with short stature
Authors: Zhao Qianqian , He Mingming , Zhang Mei , Chu Yuntian , Ban Bo 
Published: November 2023

BackgroundEvidence regarding the relationship between sleep duration and blood pressure is controversial. Therefore, the aim of this study was to investigate the relationship between sleep duration and blood pressure in children with short stature.MethodsA total of 1,085 participants with short stature were enrolled from the Affiliated Hospital of Jining Medical University in China. The variables involved in this study included sleep duration, anthropometric indicators and biochemical parameters. Sleep duration was evaluated in a face-to-face interview.ResultsThe average age of the 1,085 selected participants was 10.2 ± 3.5 years old, and approximately 763 (70.32%) of them were male. The results of adjusted linear regression showed that sleep duration was negatively associated with systolic blood pressure z scores (SBP-Z) and diastolic blood pressure z scores (DBP-Z) after adjusting for confounders (β −0.07, 95% CI −0.13, −0.01 P = 0.038; β −0.05, 95% CI −0.10, −0.01 P = 0.035, respectively). A nonlinear relationship was detected between sleep duration and blood pressure, including SBP-Z, DBP-Z and mean arterial pressure z scores (MAP-Z). The inflection point of the nonlinear relationship between sleep duration and SBP-Z is 10 h, and the inflection point of DBP-Z and MAP-Z is 8 h.ConclusionThis study revealed a nonlinear relationship between sleep duration and blood pressure in children with short stature. The findings suggest that the optimal sleep duration in children with short stature was 8–10 h, and sleep durations either too short or too long were associated with increased blood pressure levels.
Prosocial behavior among human workers in robot-augmented production teams—A field-in-the-lab experiment
Authors: Gorny Paul M. , Renner Benedikt , Schäfer Louis 
Published: November 2023

IntroductionHuman-machine interaction has raised a lot of interest in various academic disciplines, but it is still unclear how human-human interaction is affected when robots join the team. Robotics has already been integral to manufacturing since the 1970s. With the integration of AI, however, they are increasingly working alongside humans in shared spaces.MethodsWe conducted an experiment in a learning factory to investigate how a change from a human-human work context to a hybrid human-robot work context affects participants' valuation of their production output as well as their pro-sociality among each other. Learning factories are learning, teaching, and research environments in engineering university departments. These factory environments allow control over the production environment and incentives for participants.ResultsOur experiment suggests that the robot's presence increases sharing behavior among human workers, but there is no evidence that rewards earned from production are valued differently.DiscussionWe discuss the implications of this approach for future studies on human-machine interaction.
Accuracy of low-field magnetic resonance imaging for differentiating intervertebral disc extrusions and protrusions at the lumbosacral disc space in dogs
Authors: Shing Hannah , Caine Abby , Cherubini Giunio Bruto , Sparks Tim , Housley Daniel 
Published: November 2023

Introduction/PurposeMRI features differentiating extrusion from protrusion in thoracolumbar discs have been published, however little specifically evaluates the lumbosacral disc. The high prevalence of degenerative changes in apparently normal animals complicates assessment of this region and features relevant elsewhere in the spine may not apply. The aims of this study were to determine the accuracy of MRI in differentiating IVDE and IVDP at the lumbosacral disc space in dogs and determine which MRI characteristics discriminate between IVDE and IVDP.MethodMRI examinations from dogs with surgically confirmed IVDE or IVDP at the lumbosacral disc space were collected retrospectively (2011–2019). Two radiologists independently recorded a diagnosis of IVDE or IVDP, gave a confidence rating, and evaluated specific MRI features. Univariable statistical analysis was performed to identify which MRI characteristics might help distinguish IVDE from IVDP.Results117 dogs with lumbosacral IVDE (n = 16) or IVDP (n = 101) were included. Features associated with IVDE were in concordance with previous studies and included interruption of the dorsal annulus, suspected epidural hemorrhage, dispersed (rather than confined) intervertebral disc herniation on T2W sagittal images, lateralized intervertebral disc herniation and displacement of the cauda equina. Overall diagnostic accuracy was 68.8% and interobserver agreement was fair (κ = 0.37), which is lower than has been reported in thoracolumbar disc herniation, but accuracy increased to 85.3% with substantially improved agreement (κ = 0.87) in “confident” diagnoses.Discussion/ConclusionMRI characteristics used in differentiating thoracolumbar IVDE and IVDP can be extrapolated to the lumbosacral intervertebral disc space, but diagnostic accuracy in low-field MRI is lower than previously reported in herniations involving the thoracolumbar spine.
Ozonated saline intradermal injection: promising therapy for accelerated cutaneous wound healing in diabetic rats
Authors: Hesham Ahmed , Abass Marwa , Abdou Haanin , Fahmy Reham , Rashad Maha M. , Abdallah Abdelnaser A. , Mossallem Wael , Rehan Ibrahim F. , Elnagar Asmaa , Zigo František , Ondrašovičová Silvia , Abouelnaga Ahmed F. , Rizk Awad 
Published: November 2023

IntroductionThe use of ozonized water is gaining importance in medicine due to its effects on hyperglycemia and wound healing mechanisms.MethodsThis experiment was conducted to assess the impacts of intradermal administration of ozonated water on acute skin wound healing in a diabetic rat model. Sixty-four adult male Wistar rats were randomly divided into two groups: an ozonated water group (O3W) and a control group (CG). Experimental diabetes was chemically induced in the rats by the intraperitoneal administration of 60 mg/kg streptozotocin. One week later, full-thickness skin surgical wounds (1 cm2) were created between the two shoulders of the rats under general anesthesia. The wounds were then daily irrigated with normal saline (CG) or intradermally injected with 1 mL of ozonated water at 10 mg/L O3W. Wound healing was evaluated through macroscopic analysis, measuring wound size, diameter, and percentage of contraction rate before wounding and at 3, 7, 9, 12, 14, 18, 21, 24, and 28 days post-wounding. On days 7, 14, 21, and 28 after induction of the wounds, the body weights and blood glucose levels of rats (8 per group) were measured before the rats were euthanized. Moreover, the morphological structure of the tissue, vascular endothelial and transforming growth factor (VEGF and TGF) affinity and gene expression were examined.ResultsThe O3W group had significantly lower blood glucose levels and wound size and gained body weight. Additionally, epithelial vascularization, stromal edema, TGF, and VEGF gene expression significantly improved in the O3W group.DiscussionTherefore, ozonated water has the potential to enhance and promote cutaneous wound healing in diabetic rats.
Risk of African swine fever virus transmission among wild boar and domestic pigs in Poland
Authors: Pepin Kim M. , Borowik Tomasz , Frant Maciej , Plis Kamila , Podgórski Tomasz 
Published: November 2023

IntroductionAfrican swine fever (ASF) is a notifiable disease of swine that impacts global pork trade and food security. In several countries across the globe, the disease persists in wild boar (WB) populations sympatric to domestic pig (DP) operations, with continued detections in both sectors. While there is evidence of spillover and spillback between the sectors, the frequency of occurrence and relative importance of different risk factors for transmission at the wildlife-livestock interface remain unclear.MethodsTo address this gap, we leveraged ASF surveillance data from WB and DP across Eastern Poland from 2014–2019 in an analysis that quantified the relative importance of different risk factors for explaining variation in each of the ASF surveillance data from WB and DP.ResultsASF prevalence exhibited different seasonal trends across the sectors: apparent prevalence was much higher in summer (84% of detections) in DP, but more consistent throughout the year in WB (highest in winter with 45%, lowest in summer at 15%). Only 21.8% of DP-positive surveillance data included surveillance in WB nearby (within 5 km of the grid cell within the last 4 weeks), while 41.9% of WB-positive surveillance samples included any DP surveillance samples nearby. Thus, the surveillance design afforded twice as much opportunity to find DP-positive samples in the recent vicinity of WB-positive samples compared to the opposite, yet the rate of positive WB samples in the recent vicinity of a positive DP sample was 48 times as likely than the rate of positive DP samples in the recent vicinity of a positive WB sample. Our machine learning analyses found that positive samples in WB were predicted by WB-related risk factors, but not to DP-related risk factors. In contrast, WB risk factors were important for predicting detections in DP on a few spatial and temporal scales of data aggregation.DiscussionOur results highlight that spillover from WB to DP might be more frequent than the reverse, but that the structure of current surveillance systems challenge quantification of spillover frequency and risk factors. Our results emphasize the importance of, and provide guidance for, improving cross-sector surveillance designs.
PENERAPAN TARGET COSTING DALAM PENGENDALIAN BIAYA PRODUKSI PADA PT CAHAYA TIMUR GARMINDO
Authors: Karisma Elma , Sedya Utami Erni Unggul , Karunia Anita 
Published: November 2023

Target costing adalah suatu sistem yang digunakan untuk menetapkan harga pokok produk sesuai dengan yang diinginkan sebagai standar dalam menentukan harga jual produk. Hal tersebut digunakan agar perusahaan dapat memperoleh laba sesuai dengan yang ditargetkan selain itu juga sebagai upaya dalam pengendalian biaya produksi pada suatu produk. Tujuan dari penelitian ini adalah untuk mengetahui hasil perhitungan biaya produksi dengan menggunakan metode target costing sebagai upaya pengendalian biaya produksi. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, studi Pustaka, serta dokumentasi. Teknik analisis data yang digunakan adalah analisis deskriptif kuantitatif dengan melalui tahap reduksi data, penyajian data, serta penarikan kesimpulan. Hasil dari penelitian menunjukkan bahwa metode target costing dapat diterapkan pada PT Cahaya Timur Garmindo sebagai sarana untuk pengendalian biaya produksi. Melalui perhitungan Target Costing dan melakukan value engineering perusahaan dapat menghemat biaya produksi secara keseluruhan sebesar Rp. 3.035.485.740. Dengan demikian perusahaan dapat mencapai laba yang diharapkan yaitu sebesar 28% dari hasil produksi pada Kuartal III 2022.

Kata kunci: target costing, pengendalian biaya produksi, biaya produksi