bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of lipogenic enzymes and plasma lipids to starvation and refeeding in the adult Japanese quail (Coturnix coturnix japonica)
Authors: Shapira Niva , Nir I. , Budowski P. 
Published: September 2005

Adult male Japanese quail (Coturnix coturnix japonica) were starved for 48 or 60 h and then refed for either 24 or 48 h. Weight and lipid content of carcass and livers were determined, as was the lipid content of plasma. In addition, the activities of acetyl-CoA carboxylase (EC6.4.1.2; CBX), fatty acid synthetase (FAS), ATP citrate lyase (EC4.1.3.8; CCE) and malate dehydrogenase (decarboxylating) (NADP) (EC1.1.1.40; ME) were assayed in liver and in abdominal and neck adipose tissues (AAT and NAT, respectively).Body-weight and carcass lipids failed to return to normal in quail that had been starved for 48 h and refed during 24 h. When starving lasted 60 h, carcass lipids almost resumed the normal level only after 48 h of refeeding. All refeeding treatments caused a 2–3-fold increase in liver weight, with a parallel rise in fat content.In the livers of the refed quail the specific activities of all enzymes, except CBX, reached or slightly exceeded pre-starvation levels. Because of liver enlargement, the total activities of the lipogenic enzymes in the starved–refed quail exceeded pre-starvation levels. In the normally-fed quail the contribution of AAT and NAT to total lipogenesis was insignificant, and in the starved–refed birds it still remained very small compared to that of liver, despite the pronounced relative increase of lipogenic enzyme activities in these adipose tissues.
Determination of protein and reactive lysine in leaf-protein concentrates by dye-binding
Authors: Walker Ann F. 
Published: September 2005

1. Twenty leaf-protein concentrates (LPC), were produced from different crops and by different processes, the latter being designed to retain maximum nutritional value of the samples.2. The establishment of conditions for the use of CI Acid Orange 12 in a commercial dye-buffer reagent for the determination of protein and reactive (available) lysine in LPC was investigated.3. Values for protein by dye-binding correlated well with those for tungstic-acid-precipitated nitrogen (×6.25).4. Some LPC samples showed a loss of reactive lysine, the greatest loss being associated with the most severe processing conditions.5. For the LPC samples studied, dye-binding provided a convenient method for the concurrent determination of protein and reactive lysine.
A comparison of the dye-binding and fluorodinitrobenzene methods for determining reactive lysine in leaf-protein concentrates
Authors: Walker Ann F. 
Published: September 2005

1. Twenty-eight leaf-protein concentrate (LPC) samples, subjected to different thermal treatments, were produced from five curd batches. For these samples, the fluorodinitrobenzene (FDNB)-reactive lysine values gave closer agreement with dye-binding lysine (DBL) than with the dye-binding capacity (DBC).2. No relationship was established between the dye-bound-after-propionylation (DBAP) and the histidine+arginine value.3. Comparison of dye-bound-protein values with those for tungstic-acid-precipitated nitrogen ×6.25 for the LPC samples showed the heat-damaged samples to lie below the regression line for the other samples.4. Reactive-lysine values by dye-binding and by FDNB methods correlated well with total lysine, but the slopes of the regression line indicated closer agreement for values for samples not damaged by heat.5. The correlation coefficients between DBC and total basic amino acids, DBC and histidine+arginine+DBL, and DBC and histidine+arginine+FDNB-reactive lysine were similar.6. There was no correlation between the lightness of colour of the LPC samples and the availability of lysine.
The fate of amino acids in isolated perfused livers of developing sheep
Authors: Savan P. M. J. , Jeacock Majorie K. , Shepherd D. A. L. 
Published: September 2005

1. In order to establish whether or not there is a change in the relative rates of anabolism and catabolism of amino acids in the livers of lambs as they mature, a study has been made in isolated perfused livers obtained from foetal, suckling and ruminating lambs. The livers were perfused in a recirculating system and a mixture of amino acids (casein hydrolysate) was infused as substrate in the presence and absence of glucagon.2. The oxygen consumption of the livers per unit weight increased as the lambs matured but the uptake of α-amino nitrogen declined. A comparison of the rates of O2consumption and substrate uptake indicated that in foetal lambs less than 20% of the amino acids could have been oxidized, whereas in ruminating lambs all the amino acids taken up could have been oxidized.3. In livers obtained from foetal lambs, the rate of urea production per unit weight of liver was approximately double that observed in ruminating lambs. Comparison of the rate of urea production and α-NH2-N uptake indicated that more than 60% of the substrate uptake was used for non-catabolic processes in the foetal lambs, whereas in ruminating animals all the α-NH2-N taken up could be accounted for as urea.4. Gluconeogenesis could not be detected in lambs at any age studied and glucagon had no effect on any of the parameters studied.
The effect of giving feeds containing soya-bean meal treated or extracted with ethanol on digestive processes in the prerurninant calf
Authors: Sissons J. W. , Smith R. H. , Hewitt D. 
Published: September 2005

1. Preruminant calves, fitted with abomasal and re-entrant ileal cannulas, were given, at intervals of 2–3 d by infusion into the absomasum, a series of five single experimental feeds containing heated soyabean flour (product B) as the only protein source. The calves were sensitized in this way to a constituent in the soya beans and by the fifth feed showed a number of digestive disturbances. Further test feeds were then given in which heated soya-bean flour was sometimes replaced by soya-bean products prepared under laboratory or commercial conditions by treating I part fat-free raw soya-bean meal with either I part (product M) or 4 parts (product L) ethanol (750 ml/l) at 78–80° and evaporating the whole mixture to dryness, or by extracting I part meal with 4 parts aqueous ethanol under similar conditions (product K). Products M, L and K were heated with steam and finely ground. Feeds prepared from casein and from commerical ethanol-extracted concentrate (product D) were also examined.2. Measurements were made of transit time through the small intestine, flow rate of ileal digesta, recovery of polyethylene glycol (a water-soluble marker added to the feed) and net nitrogen absorption up to the distal ileum. Compared to feeds based on casein, feeds containing products K, L or M showed some differences in digesta movement and N uptake, but much less disturbance than feeds containing product B. Products prepared by extracting soya-bean meal with ethanol appeared to be slightly superior to those prepared from meal treated with ethanol, but the differences were not significant.3. Weanling rats were used to assess the nutritive value of soya-bean products by an N balance method. Digestibilities (mean 0.948) and biological values (mean 0.860) obtained for products B, D, K and L did not differ significantly.4. Results confirmed that extracting soya-bean meal with hot aqueous ethanol improved its value for calf feeding and indicated that at least part of the effect was due to the destruction of a toxic constituent present in the soya bean which probably acted by inducing a gastrointestinal allergy.
Nitrogen digestion in sheep given poor-quality indigenous hill herbages
Authors: Macrae J. C. , Milne J. A. , Wilson S. , Spence Angela M. 
Published: September 2005

1. In two experiments, the sites of digestion of non-ammonia nitrogen (NAN) and the amounts of urea N recycled to the rumen were measured in mature wether sheep given diets of indigenous hill herbage (Agrostis–Festucaand heather).2. Duodenal and ileal flow values were obtained using103Ru-phenanthroline and51Cr-EDTA markers in animals prepared with simple (T-shaped) cannuals. Amounts of urea N recycled to the rumen were estimated from measurements of the transfer of plasma urea carbon into rumen bicarbonate and the production rate of rumen bicarbonate using14C-labelled urea and bicarbonate respectively.3. The flows of NAN at the duodenum and ileum were linearly related to the intake of herbage (P< 0.001). There was a net gain of non-ammonia N anterior to the duodenum on both diets (at an intake of 460 g organic matter (OM)/d, 3.7 g NAN/d onAgrostis–Festucaand 3.3 g NAN/d on heather).4. Net digestibility of NAN entering the small intestine was within a normal range on theAgrostis–Festuca(0.58 at 460 g OM intake) diet but low on the heather diet (0.043 at 460 g OM intake).5. It was calculated that at 460 g OM intake only 0.9 and 1.1 g/d respectively of the duodenal NAN on theAgrostis-Festucaand heather diets could have been derived from urea-N recycled to the rumen. Thus 2.8 g and 2.2 g/d had to be accounted for as non-urea endogenous NAN.
The mechanism of passage of endogenous urea through the rumen wall and the role of ureolytic epithelial bacteria in the urea flux
Authors: Cheng K.-.J. , Wallace R. J. 
Published: September 2005

1. The rumen urea concentration in gnotobiotic lambs lacking ureolytic bacteria was equal to that of blood.2. Bacterial urease (EC3.5.1.5.) activity in sheep fed by intraruminal and intra-abomasal infusion was inversely related to rumen ammonia concentration.3. A model is proposed for the facilitation and control of urea flux by wall-bound ureolytic bacteria.
Changes in skeletal muscle cellularity in starved and refed young rats
Authors: Hegarty P. V. J. , Kim K. O. 
Published: September 2005

1. All food was withdrawn from male weanling rats until a 40% loss of body-weight was attained. Another group of animals was treated similarly and then refed a stock diet until the original body-weight was attained.2. The body-weight loss caused a significant reduction in the weight of the heart, kidney, liver and epididymal fat pads. Refeeding produced a return to the control weight of the heart and kidney, an increase in the weight of the liver and a deficit in the weight of the epididymal fat pads.3. Body-weight loss caused a decrease in the weight of the three different muscles studied, and in the number and diameter of the fibres in each muscle. Refeeding restored the weight and cellularity of two of the three muscles to that of the control animals. The soleus muscle was heavier in the refed animals when compared to controls due to an increased fibre diameter.4. It is concluded that the decrease in the number and diameter of muscle fibres during starvation in the rat can be restored on refeeding a stock diet.
Performance of laying hens fed on practical diets containing different levels of supplemental biotin during the rearing and laying stages
Authors: Whitehead C. C. 
Published: September 2005

1. The effect of biotin supplementation of low-biotin practical diets during rearing and laying stages on performance was studied in White Leghorn-type laying hens.2. Biotin supplementation during rearing did not have any apparent effect on the condition of the bird at point-of-lay or on subsequent laying performance.3. Biotin supplementation during lay did not have any beneficial effect upon egg number, egg size or food intake or food conversion efficiency.4. Internal egg quality, as reflected by albumen height, was slightly improved by biotin supplementation of layer's diet.5. Input-output relationships were calculated for the incorporation of biotin in egg yolk.6. Metabolic measurements suggested that biotin concentration in egg yolk was the best criterion of biotin status in the hen.7. It is concluded that practical diets contain sufficient biotin to support optimum egg-laying performance in hens.
Artificial rearing of pigs
Authors: Newport M. J. , Keal H. D. 
Published: September 2005

1. Spray-dried diets were prepared containing (g/kg): dried skim-milk 665, dried whey 65, soya-bean oil 270 (diet U); or single-cell protein (Pruteen; SCP) 308, dried whey 440, soya-bean oil 252 (diet X). The diets had a crude protein (nitrogen × 6·25) content (g/kg) of 250 (diet U) and 240 (diet X), excluding nucleic acids (36 g/kg) in diet X.2. The diets were reconstituted (200 g dry matter/l) and mixtures of diets U and X prepared to give diets supplying 0 (diet U), and approximately 400 (diet V), 600 (diet W) and 800 (diet X) g crude protein from SCP/kg total protein. All diets were supplemented with vitamins, and minerals to equalize the calcium, phosphorous, sodium and potassium concentrations.3. Pigs weaned at 2 d of age were given the diets at hourly intervals on a scale based on live weight. At 28 d age the experiment was terminated and pigs killed 1 h after a feed for a study of protein digestion. Polyethylene glycol (PEG) was fed in the diets (0.5 g/l) for 24 h before slaughter.4. Performance of pigs fed on diet V was as good as on the all-milk diet U. Greater levels of replacement by SCP (diets W and X) reduced performance. Mortality was greater on the all-milk diet, but protein source had no effect on the incidence of scouring. N retention (g/d per kg live weight) was similar for all diets but declined with age.5. SCP appeared to stimulate secretion of pepsin and chymotrypsin, and reduced the pH value in digesta in the stomach. Enzyme adaptation may have been insufficient to digest high levels of SCP in the diet, and together with the decreased transit time observed using PEG as a marker, may account for the poorer performance when 600 or 800 g/kg milk protein was replaced.6. Nucleic acids from SCP were metabolized and not retained for tissue synthesis. Allantoin excretion accounted for 75% of the theoretical maximum for complete excretion of nucleic acids, and uric acid excretion was also increased.
Experimental model for in vivo determination of dietary fibre and its effect on the absorption of nutrients in the small intestine
Authors: Sandberg Ann-Sofie , Andersson H. , Hallgren B. , Hasselblad Kristina , Isaksson B. , Hultbn L. 
Published: September 2005

1. An experimental model for the determination of dietary fibre according to the definition of Trowell et al. (1976) is described. Food was subjected to in vivo digestion in ileostomy patients, and the ileostomy contents were collected quantitatively, the polysaccharide components of which were analysed by gas–liquid chromatography and the Klason lignin by gravimetric determination. The model was used for the determination of dietary fibre in AACC (American Association of Cereal Chemists), wheat bran and for studies on the extent of hydrolysis of wheat-bran fibre in the stomach and small intestine. The effect of wheat bran on ileostomy losses of nitrogen, starch and electrolytes was also investigated.2. Nine patients with established ileostomies were studied during two periods while on a constant low-fibre diet. In the second period 16 g AACC wheat bran/d was added to the diet. The ileostomy contents and duplicate portions of the diet were subjected to determinations of wet weight, dry weight, water content, fibre components, starch, N, sodium and potassium.3. The wet weight of ileostomy contents increased by 94 g/24 h and dry weight by 10 g/24 h after consumption of bran. The dietary fibre of AACC bran, determined as the increase in polysaccharides and lignin of ileostomy contents after consumption of bran, was 280 g/kg fresh weight (310 g/kg dry matter). Direct analysis of polysaccharides and lignin in bran gave a value of 306 g/kg fresh weight. Of the added bran hemicellulose and cellulose 80–100% and 75–100% respectively were recovered in ileostomy contents. There was no significant difference between the two periods in amount of N, starch and K found in the ileostomy contents. The Na excretion increased during the ‘bran’ period and correlated well with the wet weight of ileostomy contents.4. In conclusion, it seems probable that determination of dietary fibre by in vivo digestion in ileostomy patients comes very close to the theoretical definition of dietary fibre, as the influence of bacteria in the ileum seems small. Bacterial growth should be avoided by using a technique involving the change of ileostomy bags every 2 h and immediate deep-freezing of the ileostomy contents. True dietary fibre can be determined by direct analysis of polysaccharides and lignin in the food, at least in bran. Very little digestion of hemicellulose and cellulose from bran occurs in the stomach and small bowel. The 10–20% loss in some patients may be due to digestion by the gastric juice or to bacterial fermentation in the ileum, or both. The extra amount of faecal N after consumption of bran, reported by others, is probably produced in the large bowel.
Digestion in the pig between 7 and 35 d of age
Authors: Wilson R. H. , Leibholz Jane 
Published: September 2005

1. In two separate experiments, forty-four pigs weaned at 4–5 d of age were given diets containing milk or soya-bean proteins until slaughtered at 14, 28 or 35 d of age.2. The retention times of digesta in the stomach and the entire gastro-intestinal tract did not differ between pigs given pelleted diets containing milk or soya-bean protein.3. Digesta retention times in the stomach were shorter (61 v. 146 min) in pigs given a milk-protein diet in a liquid form than in pigs given the same diet in a pelleted form. The retention times in the whole gastro-intestinal tract were 42.8 v. 29.6 h on the respective diets.4. The total retention time of digesta in the gastro-intestinal tract increased with age of pigs between 14–35 d of age when given pelleted diets but not when given a liquid diet.5. The daily amount of digesta flowing through the anterior small intestine was unaffected by the source of protein. Greater endogenous secretions into the anterior small intestine were observed when pigs were fed ad lib. than when fed at 2 g nitrogen (45 g dry matter (DM))/kg live weight0.75 per d.6. The apparent digestion of DM to the ileum of pigs given milk, isolated soya-bean protein (ISP) (Promine D) or soya-bean meal (SBM) protein at 28 d of age was 0.826, 0.825 and 0.644 respectively.7. The apparent digestion of DM to the ileum of pigs given ISP (Supro 610) significantly increased with age of pigs from 0.851 at 14 d of age to 0.883 at 35 d of age. No increase was observed for pigs given milk protein.8. Differences in the digestibility of DM between protein sources were greater to the ileum than over the entire gastro-intestinal tract. The greatest increase in DM digestion from the ileum to the faeces occurred in pigs given SBM (0.644–0.874).9. Neither the protein source nor age of pigs influenced gastric pH values, the mean value being 4.05. pH increased along the small intestine and was not affected by the protein source or age of pig.
Digestion in the pig between 7 and 35 d of age
Authors: Wilson R. H. , Leibholz Jane 
Published: September 2005

1. In two experiments, forty-four pigs weaned at 4–5 d of age were given diets containing milk or soya-bean proteins until slaughtered at 14, 28 or 35 d of age.2. Additions of methionine and methionine plus lysine to the diets did not increase the proportions of these amino acids in ileal digesta.3. In the pigs given a nitrogen-free diet, ileal digesta contained more threonine, proline and glycine than in pigs given diets containing protein.4. The apparent and true digestibility of amino acids were greater when milk protein was fed than when soya-bean proteins were fed.5. The apparent digestion of amino acids to the ileum of pigs given isolated soya-bean protein (ISP; Supro 610) increased with increasing age of pigs from a mean of 0.82 at 14 d of age to 0.87 at 35 d of age.6. When milk protein was fed apparent digestibilities of methionine and lysine to the ileum were 0.912 and 0.905. The apparent digestibility of threonine to the ileum was 0.800, 0.774 and 0.504 for pigs given the milk-, ISP and soya-bean-meal (SBM)-protein diets respectively.7. Apparent digestibilities of total essential and non-essential amino acids were 0.79 and 0.69 respectively, and true digestibilities of both were 0.82.
Digestion in the pig between 7 and 35 d of age
Authors: Wilson R. H. , Leibholz Jane 
Published: September 2005

1. In two separate experiments, forty-two pigs weaned at 4–5 d of age were given diets containing milk or soya-bean protein and slaughtered at 7, 28 or 35 d of age.2. The amino acid composition of the empty body of the pigs did not vary with age or sources of protein fed.3. The nitrogen and amino acid contents of the empty body gain were calculated between 7 and 28, and 7 and 35 d of age. The retention of absorbed N was greater in pigs given a soya-bean meal (SBM) diet than in pigs given milk or isolated soya-bean protein (ISP) diets.4. The retention of total absorbed N was greater in pigs given a pelleted milk diet (0.72) than in pigs given a liquid milk diet (0.58).5. Methionine and threonine appeared to be the first two limiting amino acids, as indicated by their high incorporation into the empty body, for pigs given soya-bean proteins in Expt 3, while methionine and lysine were the first two limiting amino acids for pigs given ISP in Expt 4.6. The addition of methionine to diets in Expt 3 reduced the retention of absorbed methionine in the empty body by 43–76%. Lysine supplementation did not alter the retention of the absorbed lysine.7. Retention of arginine, proline and glycine in the body was greater than the apparent absorption of each of these amino acids.
Phosophorus kinetics in the sheep
Authors: Grace N. D. 
Published: September 2005

1. Ten castrated young adult sheep were brought indoors and given lucerne chaff (four animals with a phosphorus intake of 2.40 g/d) and fresh Ruanui ryegrass (six animals with a P intake of 3.72 g/d) to determine various kinetic indices of their P metabolism.2. The results obtained from combined nutritional balance and 32P radioactivity measurements were analysed using the simulation, analysis and modelling computer (SAAM) program. Since the plasma P specific activity curve was described by at least four exponential terms, a four compartmental model (M1, M2, M3 and M4) was used to describe the experimental values.3. The mean mass of the total exchangeable P pool (MT) for the lucerne chaff-fed and Ruanui ryegrass-fed sheep was 25.5 and 28.8 g respectively, while the total P transport (VT) to the exchangeable pool (MT) was markedly lower for sheep given lucerne chaff compared to those given Ruanui ryegrass (54.1 v. 111.3 mg/kg body-weight per d). Likewise the rate of P absorption (Va), faecal endogenous loss of P (Vf), urinary P excretion (Vu) as well as the movement of P to (Vo+) and from (Vo−) the skeleton and soft tissue was also lower in the lucerne chaff-fed sheep.4. The availability of P, that is the proportion of ingested P which is absorbed, was 0.56 and 0.62 for the sheep given chaff and fresh herbage respectively.5. The P metabolism of one sheep (sheep no. 30) differed from the other sheep in that its plasma inorganic P concentration was higher (95 v. 54 mg/l). This was associated with a larger first compartment (M1), a more rapid P transport between M1 and M2, an increase in (Va) and a marked increase in (Vu).