bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic variation in populations of the endangered fish Ladigesocypris ghigii and its implications for conservation
Authors: MAMURIS ZISSIS , STOUMBOUDI MARIA TH. , STAMATIS COSTAS , BARBIERI ROBERTA , MOUTOU KATERINA A. 
Published: August 2005

Summary1. The genetic variation of the endangered freshwater fish Ladigesocypris ghigii, endemic to the island of Rhodes (Greece), was investigated for nine populations, originating from seven different stream systems and a reservoir, both at the mtDNA and nuclear level, in order to suggest conservation actions.2. Both restriction fragment length polymorphism analysis of five segments of mitochondrial DNA (ND‐5/6, COI and 12S‐16S rRNA) amplified by polymerase chain reaction, and random amplified polymorphic DNA analysis, revealed extremely low levels of intra‐population polymorphism. It is highly likely that the low intra‐population variability is the result of successive bottleneck events evident in shrinkage and expansion of the populations year after year, which may have led to a complete loss of several genotypes and haplotypes, and an increased degree of inbreeding.3. Inter‐population genetic structuring was high, with fixation of haplotypes within six of the nine populations and fixation of alleles within populations originating from different waterbodies. It is probable that all haplotypes and/or alleles found were initially represented in all populations. However, because of the long time of isolation coupled with successive bottleneck and subsequent genetic drift, common mtDNA haplotypes and alleles among the populations may have become rare or extinct through stochastic lineage loss.4. Although nucleotide divergence among haplotypes was very shallow, half of the haplotypes recorded (three of six), resulted from nucleotide changes on the 12S–16S rRNA segments, which are the most conserved part of the mitochondrial genome. This fact may indicate that the observed genetic variation did not necessarily result only from the retention of ancestral polymorphism, but may have arisen through mutation and complete lineage sorting over a relatively small number of generations, once the populations had become isolated from one another.5. Our data suggest that two of the L. ghigii populations may be on independent evolutionary trajectories. Considering that each population appears so far well adapted within each site, all populations should be managed and conserved separately.
Survival and development of five species of cyclopoid copepods in relation to food supply: experiments with algal food in a flow‐through system
Authors: HOPP ULRICH , Maier GERHARD 
Published: August 2005

Summary1. Cyclops spp. generally develop and grow during favourable food conditions in spring and undergo a diapause in summer, while Acanthocyclops robustus, Mesocyclops leuckarti and Thermocyclops crassus develop and grow in summer when they face poorer food conditions and more competition from Cladocera. Since nauplii are the bottleneck in copepod development, we tested the hypothesis that Cyclops abyssorum and C. vicinus nauplii have higher food requirements for survival and development than the nauplii of A. robustus, M. leuckarti and T. crassus. We also tested survivorship and development from hatching to adulthood.2. Survivorship and development of the copepods was studied in a flow‐through system using five concentrations of the phytoflagellate Chlamydomonas reinhardtii in the range from 1 × 104 to 4.5 × 105 cells mL−1 (approximately 0.5–22.5 mg C L−1).3. Nauplii of both species of Cyclops died at intermediate to low (C. abyssorum) and low (C. vicinus) food concentrations, while nauplii of A. robustus, M. leuckarti and T. crassus survived at all concentrations.4. The negative effects of low food concentration were also reflected in development. In C. abyssorum and C. vicinus, development duration increased at low food concentration while development was much less affected in A. robustus and T. crassus. Mesocyclops leuckarti was intermediate between Cyclops spp. and A. robustus/T. crassus, with an increase in development duration at the lowest food concentration.5. Our results support the hypothesis that summer diapause in Cyclops spp. has developed as a strategy to avoid a food bottleneck for nauplii.
Zooplankton of turbid and hydrologically dynamic prairie rivers
Authors: THORP JAMES H. , MANTOVANI SARA 
Published: August 2005

Summary1. Compared with rivers in more humid, forested ecoregions of eastern and midwestern U.S.A., rivers in semi‐arid grassland of the U.S. Great Plains tend to be relatively shallow, more variable in discharge, and characterised by high suspended sediment loads. Although critical life stages of fish in prairie rivers probably depend at least partially on zooplanktonic food, data on community and distributional patterns of potamoplankton in these widespread ecosystems are almost entirely absent.2. We examined summer zooplankton distribution in five prairie rivers (Arkansas, Kansas, Platte, Elkhorn, and Niobrara Rivers) spread over six degrees of latitude during 2003–2004. We compared our results from 126 samples with previously collected data from the Ohio and St Lawrence Rivers in forested ecoregions and correlated differences with abiotic environmental conditions.3. The importance of hydrological retention zones to stream biota has been recently demonstrated for rivers with quasi‐permanent islands and slackwater regions, but the importance of slackwaters formed by ephemeral sandbar islands in prairie rivers is unknown. We evaluated the role of hydrological retention for planktonic rotifers, cladocera, and copepods in the Kansas River during the summer of 2004.4. Zooplankton assemblages were extremely similar among prairie rivers (Sorensen Dissimilarity Index: mean = 0.07) but moderately disparate for comparisons of prairie versus forested‐basin rivers (mean = 0.50).5. Total zooplankton densities in prairie rivers (approximately 81 L−1) were intermediate between the Ohio (approximately 92 L−1) and St Lawrence Rivers (approximately 43 L−1), but relative abundances were significantly different. Rotifers represented >99% of zooplankton individuals in grassland rivers, but only approximately 37–68% in other rivers. Rotifer species richness was lower in prairie rivers, but relative abundances of common genera were much less skewed compared with eastern rivers where Polyarthra dominated rotifer assemblages (41–73%).6. For comparisons among rivers, rotifers were significantly more abundant in turbid rivers, while microcrustaceans were less dense. However, for comparisons within the Kansas River over time, rotifer densities were inversely related to turbidity. We hypothesise that rotifers indirectly benefit from river turbidity because their food competitors (cladocera) and predators (e.g. cyclopoid copepods and visually feeding fish) are relatively more susceptible to suspended sediments.7. Crustacean densities were positively related to the degree of hydrological retention (negatively to current velocities) throughout the study, but rotifer densities were significantly depressed by current velocities only when river discharge was high, making slackwaters that much more valuable. Ephemeral sandbars may not provide sufficient hydrological retention in time and space to sustain viable crustacean populations, but they are adequate to help sustain growth of rotifer populations.
Combined effects of water column nitrate enrichment, sediment type and irradiance on growth and foliar nutrient concentrations of Potamogeton alpinus
Authors: BOEDELTJE GER , SMOLDERS ALFONS J. P. , ROELOFS JAN G. M. 
Published: August 2005

Summary1. High water column NO3− concentrations, low light availability and anoxic, muddy sediments are hypothesised to be key factors hampering growth of rooted submerged plants in shallow, eutrophic fresh water systems. In this study, the relative roles and interacting effects of these potential stressors on survival, growth, allocation of biomass and foliar nutrient concentrations of Potamogeton alpinus were determined in a mesocosm experiment using contrasting values of each factor (500 versus 0 μmol L−1 NO3−; low irradiance, corresponding to the eutrophic environment, versus ambient irradiance; and muddy versus sandy sediment).2. Low irradiance, high NO3− and sandy sediment led to reduced growth. In a muddy sediment, plants had lower root : shoot ratios than in a sandy sediment.3. Growth at high NO3− and on the sandy sediment resulted in lower foliar N and C concentrations than in the contrasting treatments. The C : N ratio was higher at high NO3− and on the sandy sediment. Foliar P was higher on the muddy than on the sandy sediment but was not affected by irradiance or NO3−. The N : P ratio was lowest at high NO3− on the sandy sediment.4. Total foliar free amino acid concentration was lowest on sand, low irradiance and high NO3−. Total free amino acid concentration and growth were not correlated.5. Turbidity and ortho‐PO43− concentration of the water layer were lower at high water column NO3− indicating that the growth reduction was not associated with increased algal growth but that physiological mechanisms were involved.6. We conclude that high water column NO3− concentrations can significantly reduce the growth of ammonium preferring rooted submerged species such as P. alpinus, particularly on sediments with a relatively low nutrient availability. Further experiments are needed to assess potential negative effects on other species and to further elucidate the underlying physiological mechanisms.
Monte‐Carlo Bayesian look‐ahead inversion of walkaway vertical seismic profiles
Authors: Malinverno Alberto , Leaney W. Scott 
Published: August 2005

ABSTRACTWe describe a method to invert a walkaway vertical seismic profile (VSP) and predict elastic properties (P‐wave velocity, S‐wave velocity and density) in a layered model looking ahead of the deepest receiver. Starting from Bayes's rule, we define a posterior distribution of layered models that combines prior information (on the overall variability of and correlations among the elastic properties observed in well logs) with information provided by the VSP data. This posterior distribution of layered models is sampled by a Monte‐Carlo method. The sampled layered models agree with prior information and fit the VSP data, and their overall variability defines the uncertainty in the predicted elastic properties. We apply this technique first to a zero‐offset VSP data set, and show that uncertainty in the long‐wavelength P‐wave velocity structure results in a sizable uncertainty in the predicted elastic properties. We then use walkaway VSP data, which contain information on the long‐wavelength P‐wave velocity (in the reflection moveout) and on S‐wave velocity and density contrasts (in the change of reflectivity with offset). The uncertainty of the look‐ahead prediction is considerably decreased compared with the zero‐offset VSP, and the predicted elastic properties are in good agreement with well‐log measurements.
Quasi‐2D hybrid joint inversion of seismic and geoelectric data
Authors: De Nardis R. , Cardarelli E. , Dobroka M. 
Published: August 2005

ABSTRACTMany joint inversion schemes use 1D forward modelling in the integrated interpretation of various geophysical data. In extending the joint inversion approach to the investigation of 2D structures, the discretization of the model parameters and the appropriate choice of the forward‐modelling procedure play a very important role. In this paper, a hybrid seismic–geoelectric joint inversion method is proposed for the investigation of 2D near‐surface geological structures. The electric and seismic models are coupled together through the use of common boundaries between the adjacent layers. Assuming a 2D model composed of homogeneous layers with curved boundaries, a fast ray‐tracing algorithm is used for the calculation of refraction seismic traveltime data. In the geoelectric forward modelling, a locally 1D approximation is used. The boundary surfaces are written in the form of series expansion; the inversion algorithms are formulated for the expansion coefficients and the petrophysical parameters as unknowns. Two versions of the inversion method are proposed: in versions A and B, interval‐wise constant functions and Chebyshev polynomials are, respectively, used as basis functions of the series expansion. The versions are tested by means of synthetic and in situ measured data. The tests show that both methods are stable and accurate.
Zero‐offset P–S energy in horizontally layered media
Authors: Artola Fredy A.V. , Leiderman Ricardo , Fontoura Sergio A.B. 
Published: August 2005

ABSTRACTThe existence of non‐zero reflectivity for zero‐offset PS waves in horizontally layered media is discussed. Field measurements have suggested the occurrence of this phenomenon. We show that, in some cases, if anisotropy is considered in the problem, this reflectivity can be predicted. By using an approximated formulation to determine the coefficient of reflection in arbitrarily anisotropic media, it is clear that, for some elastic arrangements of the subsurface, reflected energy is associated with the converted wave resulting from normal P‐wave incidence.
Dry‐matter yields and crude protein and rumen‐degradable protein concentrations of three Arachis pintoi ecotypes at different stages of regrowth in the humid tropics
Authors: Villarreal M. , Cochran R. C. , Villalobos L. , Roja‐Bourrillón A. , Rodríguez R. , Wickersham T. A. 
Published: August 2005

AbstractWell‐established stands of three ecotypes of Arachis pintoi (CIAT 17434, 18744 and 18748) were harvested from replicated plots (three blocks, each containing three plots for each ecotype) during the two dominant seasons (dry and wet) of the low altitude, humid tropics of Costa Rica. Each plot was further divided into six subplots so that, within each season, samples corresponding to 4, 6, 8, 10, 12 or 14 weeks of regrowth could be collected. For each harvest, dry matter (DM) yield of the leaf, stem and whole plant, and the leaf:stem ratio, were recorded. Samples of the whole plant were analysed for crude protein (CP), rumen‐degradable (RDP) and rumen‐undegradable protein (RUP) concentrations. DM yield of the leaf, stem and whole plant increased with advancing period of regrowth but the effects of period of regrowth varied somewhat among ecotypes and across seasons. Generally, DM yield was greater during the wet than during the dry season. The greatest difference between ecotypes for stem and total DM yields was evident during the dry season. In general, DM contents were low in the whole plant, leaf and stem samples (<220 g kg−1) and increased with increasing period of regrowth. Increases in leaf:stem ratio were most dramatic during the dry season with greater periods of regrowth, although the ratio was fairly constant during the wet season. Whole‐plant CP concentration was relatively high after short periods of regrowth (up to 279 g kg−1 DM) but declined with longer periods of regrowth; the relative decline was much greater during the dry season. The RDP concentration was relatively constant during the wet season (mean 115 g kg−1 DM), but declined with longer periods of regrowth during the dry season (range 194–111 g kg−1 DM). In general, the concentrations of RDP, on a CP basis, were greater during the dry season and ranged from 590 to 700 g kg−1 CP. Season, ecotype and period of regrowth all exerted an effect on RUP concentrations.
The influence of grass and maize silage quality on apparent diet digestibility, metabolizable energy concentration and intake of finishing beef cattle
Authors: Kirkland R. M. , Steen R. W. J. , Gordon F. J. , Keady T. W. J. 
Published: August 2005

AbstractThe effects of offering a range of grass silages and mixtures of grass and maize silages on the intake of beef cattle were studied. Four grass silages (GS1, GS2, GS3 and GS4) were used. Grass silage 1 was ensiled from a second regrowth in mid‐late September and treated with an inoculant additive. Grass silages 2, 3 and 4 were ensiled, without additive, from a primary regrowth harvested in early July, late May and mid‐June respectively. Wilting periods were 8, 30, 36 and 36 h for GS1, GS2, GS3 and GS4 respectively. Grass silages 1, 2 and 3 were precision chopped and ensiled in bunker silos, while GS4 was ensiled in round bales. The DM content (g kg−1) and starch concentration (g kg−1 DM) of the three maize silages (MS1, MS2 and MS3) used were 256 and 128, 256 and 184, and 402 and 328 for MS1, MS2 and MS3 respectively. Seventy‐two Charolais and Limousin cross‐bred steers were used in a changeover design with two 4‐week periods. The study consisted of sixteen treatments incorporating the four grass silages fed alone and with the three maize silages arranged as a 4 × 4 factorial design. The grass silage and maize silage mixtures were offered in a ratio of 0·60:0·40 (DM basis) once daily using individual Calan gates. All silages were offered ad libitum with 3 kg per head per day of a concentrate supplement. Dry matter and metabolizable energy (ME) intakes were highest with diets based on grass silage GS4 compared with diets containing the other grass silages. Metabolizable energy intakes of diets containing no maize silage, and those based on MS1 and MS2, were similar (P > 0·05) but lower than that of diets containing MS3. Only limited increases were found in DM and ME intakes with the inclusion of maize silage in grass silage‐based diets while offering high‐quality grass silage (assessed in terms of DM content, and fibre and N concentrations) promoted high voluntary intakes.
Species composition of ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) mixtures using various combinations of n‐alkanes
Authors: Cortes C. , Damasceno J. C. , Bechet G. , Prache S. 
Published: August 2005

AbstractThis study was conducted to evaluate the reliability of the n‐alkane technique for estimating the species composition of Lolium perenne (ryegrass, L) and Festuca arundinacea (tall fescue, F) mixtures. Samples of L and F were collected in May (period 1) and June 2003 (period 2) on mono‐specific swards that were maintained at a height of 9 cm. Seven sets of mixtures containing controlled proportions of L and F were then prepared: 1·00:0, 0·80:0·20, 0·60:0·40, 0·50:0·50, 0·40:0·60, 0·20:0·80 and 0:1·00 of L:F, respectively, in the mixture. The proportion of each species in the mixtures was estimated from measured n‐alkane concentrations, and then compared with the actual known proportion. Various n‐alkane combinations were tested and compared to establish which combination gave the best estimate. Total n‐alkane concentrations, and especially C29‐ and C31‐alkane concentrations, were higher in period 1 than in period 2 for both species. The best n‐alkane combination varied between periods, as did the effectiveness of C29‐alkane in discriminating between L and F. The species composition of the mixtures was satisfactorily estimated for the majority of n‐alkane combinations, providing the combination included n‐alkanes with contrasting profiles between both species. A systematic overestimation of the proportion of ryegrass was observed for the 0·50:0·50 mixture.
Apparent digestibility and nitrogen utilization of diets based on maize silage harvested at three stages of maturity and fed to steers
Authors: Browne E. M. , Juniper D. T. , Bryant M. J. , Beever D. E. 
Published: August 2005

AbstractAdvancing maize crop maturity is associated with changes in ear‐to‐stover ratio which may have consequences for the digestibility of the ensiled crop. The apparent digestibility and nitrogen retention of three diets (Early, Mid and Late) containing maize silages made from maize of advancing harvest date [dry matter (DM) contents of the maize silages were 273, 314 and 367 g kg−1 for the silages in the Early, Mid and Late diets respectively], together with a protein supplement offered in sufficient quantities to make the diets isonitrogenous, were measured in six Holstein–Friesian steers in an incomplete Latin square design with four periods. Dry‐matter intake of maize silage tended to be least for the Early diet and greatest for the Medium diet (P = 0·182). Apparent digestibility of DM and organic matter did not differ between diets. Apparent digestibility of energy was lowest in the Late diet (P = 0·057) and the metabolizable energy concentrations of the three silages were calculated as 11·0, 11·1 and 10·6 MJ kg−1 DM for the Early, Medium and Late diets respectively (P = 0·068). No differences were detected between diets in starch digestibility but the number of undamaged grains present in the faeces of animals fed the Late diet was significantly higher than with the Early and Mid diets (P = 0·006). The apparent digestibility of neutral‐detergent fibre of the diets reduced significantly as silage DM content increased (P = 0·012) with a similar trend for the apparent digestibility of acid‐detergent fibre (P = 0·078). Apparent digestibility of nitrogen (N) was similar for the Early and Mid diets, both being greater than the Late diet (P = 0·035). Nitrogen retention did not differ between diets. It was concluded that delaying harvest until the DM content is above 300 g kg−1 can negatively affect the nutritive value of maize silage in the UK.
Potassium cycling and losses in grassland systems: a review
Authors: Kayser M. , Isselstein J. 
Published: August 2005

AbstractCycling of potassium in grassland systems has received relatively little attention in research and practice in recent years. Balanced nutrient systems require consideration of nutrients other than nitrogen (N). Potassium (K) is needed in large amounts and is closely related to N nutrition. In intensive dairy farming, surpluses of K arise from the input of concentrates and fertilizer and are returned to the grassland and may lead to increasing K content in the soil. Organic farming, on the other hand, is characterized by limitations in input of nutrient sources and quantities. Leaching of K from grassland is usually low, but high levels of available soil K, high K input from fertilizer or at urine patches lead to increasing losses. High K inputs have a negative influence on Mg and Ca uptake by plants and can cause accelerated leaching of these cations. High levels of K have been associated with inducing nutrition‐related dairy cow health problems such as milk fever (hypocalcaemia) and grass tetany (hypomagnesaemia). This review gives an overview of the cycling of potassium and related cations in grassland systems especially with regard to leaching losses and identifies limitations to knowledge.
Assessment of regional variation in climate on the management of dairy cow systems in Ireland using a simulation model
Authors: Fitzgerald J. B. , Brereton A. J. , Holden N. M. 
Published: August 2005

AbstractA dairy system simulator, Dairy_sim, was designed to assess the interactions between climate and management in spring‐calving milk production systems based on the grazing of grass pastures. The simulator comprises three main components: a grass herbage growth model, an intake and grazing behaviour model, and a nutrient demand model. The simulator was initially parameterized using the Irish National Dairy Blueprint. Sensitivity analysis indicated that the simulator was most sensitive to stocking rate, milk output per cow and nitrogen fertilizer inputs, but less sensitive to other variables. Field data from four grazing systems were used to test the simulator and it was concluded that Dairy_sim was suitable for evaluating the interaction of climate and management for rotational grazing dairy systems based on perennial ryegrass pastures with Friesian cows. The simulator, Dairy_sim, was then used to evaluate the effects of the regional climates of Ireland on system management. The results indicated that, between regions, herbage production at the same input of nitrogen may vary proportionally by 0·10 and that the length of the grazing season may vary by 0·25. It was concluded that the simulator could be a useful tool for developing region‐specific dairy production blueprints.