bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Term Stability of Cord Blood Units After 29 Years of Cryopreservation: Follow-Up Data From the José Carreras Cord Blood Bank
Authors: Liedtke Stefanie , Többen Sabine , Gressmann Holger , Meyer Andrea , Verde Pablo E , Gluckman Eliane , Kogler Gesine 
Published: November 2023

Abstract
The José Carreras Cord Blood Bank (CBB) located in Düsseldorf as of today stores 21 215 active cryopreserved cord blood units (CBUs) applicable as a source for hematopoietic stem cell (HSC) transplantation. Since the success of transplantation outcomes is mainly dependent on the cord blood quality, typical parameters are evaluated by a Stability Monitoring Program specified by the FACT Standards. The longest expiration time determined to date is 29 years for unseparated units, 25 years for manual and 18 years for automated volume-reduced units licensed by the Paul-Ehrlich Institute. According to the CBB stability program TNC count, TNC recovery, TNC viability, CD34+7AAD− viability, CD45+7AAD− viability and CFC count were determined for all 3 processing methods applied over time. As a measure of stability, unseparated units (processed 1993-1998) revealed a mean TNC viability of 88.91 ± 5.01% after 29 years of cryopreservation versus manual volume-reduced CBUs (processed 1998-2005) with a mean of 84.22 ± 10.02% after 25 years of cryopreservation versus automated volume-reduced CBUs (processed since 2005) with a mean of 88.64.91 ± 3.91% after 18 years of cryopreservation. In addition, these relevant parameters were retrospectively analyzed for released transplants in correlation to the storage time. Moreover, the follow-up data of recipients from CBUs cryopreserved directly (unseparated) versus CBUs cryopreserved after manual versus automated volume-reduction are presented here demonstrating an earlier engraftment in both volume-reduced groups as compared to unseparated CBUs. By this retrospective analysis, key questions are discussed regarding cord blood parameters in relation to processing methods, engraftment, and patient age (children and adults).
3D Spheroids Facilitate Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells via p300-Mediated H3K56 Acetylation
Authors: Yu Yanrong , Huang Haina , Ye Junsong , Li Yumei , Xie Renjian , Zeng Liping , Huang Yushan , Zeng Tai , Luo Dan , Zhong Jianing , Peng Weijie 
Published: November 2023

Abstract
Hepatocyte-like cells (HLCs) that are differentiated from mesenchymal stem cells (MSCs) provide a valuable resource for drug screening and cell-based regeneration therapy. Differentiating HLCs into 3D spheroids enhances their phenotypes and functions. However, the molecular mechanisms underlying MSCs hepatogenic differentiation are not fully understood. In this study, we generated HLCs from human adipose-derived mesenchymal stem cells (hADMSCs) in both 2D and 3D cultures. We performed an acetyl-proteomics assay on the HLCs derived from both 2D and 3D differentiation and identified a differential change in H3K56 acetylation between the 2 differentiated cells. Our findings revealed that 3D differentiation activated ALB gene transcription by increasing the acetylation level of H3K56, thereby enhancing the phenotypes and functions of HLCs and further promoting their maturation. Notably, inhibiting p300 reduced the acetylation level of H3K56 during hepatogenic differentiation, leading to decreased phenotypes and functions of HLCs, whereas activation of p300 promoted hepatogenic differentiation, suggesting that p300 plays a critical role in this process. In summary, our study demonstrates a potential mechanism through which 3D spheroids differentiation facilitates hADMSCs differentiation into HLCs by promoting p300-mediated H3K56 acetylation, which could have significant clinical applications in liver regeneration and disease modeling.
Refuge for Rover: A Social Return on Investment of a Program Assisting Victim–Survivors of Domestic and Family Violence with Their Pets
Authors: Ma Gemma Catherine , Ravulo Jioji , McGeown Ursula 
Published: November 2023

Abstract
Pets are likely to be present in as many as 70 percent of domestic and family violence (DFV) cases, and the bond between victim–survivors and their animals can be particularly strong. Animals can also be victims of DFV, and concern for their animal’s safety can cause victims to delay leaving their abusive situations. Programs like the Royal Society for the Prevention of Cruelty to Animals New South Wales Domestic Violence Program, which provide temporary accommodation for pets, can enable victim–survivors and their children to plan their escape and access safety. This article evaluated the program using social return on investment methodology. Evidence on outcomes experienced by three stakeholder groups were collected from 15 stakeholder interviews and 37 program client questionnaires. Concern for their animals had caused 54 percent of clients to delay leaving their abusive situation, most for six months or more. Program clients, their children, and their animals experienced improved safety, mental health and well-being, and physical health because of the program. The alternative described by clients was often homelessness or living in their car. In total an estimated AUD$9.65 of social value was created for every AUD$1 invested into the program.
Territorial Expansionists and Troublesome Locals: Daniel Clark at New Orleans and John Christian Schultz at Red River
Authors: Vernet Julien 
Published: November 2023

Abstract
The United States and Canada began major efforts to acquire territory in North America’s interior in the nineteenth century. In 1803, the United States purchased approximately 827,192 square miles of territory from France. Canada began to govern 1,409,900 square miles of territory known as Rupert’s Land in 1869. Historical accounts of American and Canadian officials’ efforts in Washington and Ottawa to obtain these vast territories are abundant. Historians have devoted less attention, however, to expansionists who supported American expansion in Louisiana and Canadian expansion in Rupert’s Land. Daniel Clark, U.S. Consul to New Orleans, used his office to promote American acquisition of Spanish Louisiana. Canadian John Christian Schultz, an influential “doctor” and businessperson at Red River, became the owner of the only newspaper in the settlement and used it to advocate for Canadian possession of Rupert’s Land. Clark arrived in Louisiana from Ireland in 1786 and Schultz from Upper Canada between 1859 and 1861. Clark and Schultz were opportunists who understood that American and Canadian territorial ambitions presented them with opportunities to advance their business and political careers. Far less important to both men were the futures of other residents of New Orleans and Red River. Clark and Schultz for example, both argued that representative local government should not be immediately introduced in their regions.
Molecular Adsorbent Recirculating System for Acute Liver Failure in a New Pediatric-Based Extracorporeal Liver Support Program
Authors: Baker David R. , Mac Helen , Steinman Benjamin , Soshnick Sara H. , Frager Shalom Z. , Goilav Beatrice , Kogan-Liberman Debora , Ovchinsky Nadia , Shlomovich Mark 
Published: November 2023

IMPORTANCE:
Acute liver failure (ALF) carries significant morbidity and mortality, for both pediatric and adult patients. Albumin dialysis via the molecular adsorbent recirculating system (MARS) is a form of extracorporeal liver support (ELS) that can reduce hepatic encephalopathy (HE), a main driver of mortality in ALF. However, data on MARS and its benefit on mortality have been inconsistent.

OBJECTIVES:
We sought to report our experiences and patient outcomes from the first 2 years of operation of a new ELS program, within an established pediatric liver transplantation center.

DESIGN, SETTING, AND PARTICIPANTS:
Retrospective review of outcomes in pediatric and adult patients treated with MARS therapy for ALF, from 2021 to 2022.

MAIN OUTCOMES AND MEASURES:
Outcomes included reduction in HE and biochemical markers of ALF after MARS therapy, survival, and transplant-free survival. Comparisons were made via Wilcoxon signed-rank test.

RESULTS:
Five pediatric and two adult patients underwent MARS for ALF. Ages ranged from 2 to 29 years. Overall, 21 MARS runs were performed (median 3 runs per patient, 12.4 hr per run [interquartile range, IQR 10.1–17]). Overall survival was 85.7%, and transplant-free survival was 71.4%. There was a statistically significant reduction in HE score with MARS therapy (median 3 [IQR 3–4] to 1 [IQR 0–1], p = 0.03), and in ALF biomarkers including ammonia (256 µL/dL [195–265] to 75 µL/dL [58–101], p = 0.02), aspartate aminotransferase (6,362 U/L [920–8,305] to 212 U/L [72–431], p = 0.02), alanine aminotransferase (8,362 U/L [3,866–9,189] to 953 U/L [437–1,351], p = 0.02), and international normalized ratio (4.5 [3.3–6.7] to 1.3 [1.2–1.4], p = 0.02).

CONCLUSIONS AND RELEVANCE:
MARS therapy for ALF was well tolerated by both pediatric and adult patients, and resulted in significant improvement in clinical and biochemical parameters. We demonstrated encouraging overall and transplant-free survival, suggesting that early initiation of MARS with relatively long and frequent cycle times may be of significant benefit to ALF patients, and is worthy of additional study in larger cohorts.
Arterial stiffness of the aorta and iliofemoral artery and their responses to nitroglycerin administration in myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits
Authors: Katsuda Shin-ichiro , Horikoshi Yuko , Shiomi Masashi , Kitajima Shuji , Ito Toshiro , Hazama Akihiro , Shimizu Tsuyoshi , Shirai Kohji 
Published: November 2023

Objectives:
The role of hypercholesterolemia in arterial stiffness, which usually reflects the progression of atherosclerosis has not been fully investigated. To clarify the meaning of arterial stiffness in hypercholesterolemia, we evaluated arterial stiffness in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits by using new arterial stiffness indices of the aorta and common iliac to femoral artery. The new arterial stiffness indices of both arteries were determined by the application of the theory of cardio-ankle vascular index (CAVI) to the aorta (aBeta) and ilio-femoral artery (ifBeta). Furthermore, the responses of both indices to nitroglycerin (NTG) administration were compared between WHHHMI and normal rabbits.

Design and methods:
aBeta and ifBeta of WHHLMI and normal rabbits were measured under anesthesia. Pulse wave velocity in the whole aorta (aPWV) and ilio-femoral artery (ifPWV), blood pressure, and other parameters were measured before and after administration of NTG (50–120 μg/kg/min) every 1 for 5 min.

Results:
Atherosclerotic lesions were observed in the aorta, but a little in the ilio-femoral artery in WHHLMI rabbits. Compared with normal rabbits, aBeta was significantly higher, but ifBeta was lower in WHHLMI rabbits. When NTG was administered, ifBeta decreased significantly in both groups; however, aBeta increased in normal rabbits, but remained unchanged in WHHIMI rabbits.

Conclusion:
These findings suggested that hereditary hypercholesterolemia in rabbits did not uniformly enhance arterial stiffness in elastic artery and muscular artery. The responses to NTG were also different between two arteries. The mechanism of these different responses needs further studies.
Midlife hypertension is a risk factor for some, but not all, domains of cognitive decline in later life: a systematic review and meta-analysis
Authors: Joyce Oisín Cormac , McHugh Clíodhna , Mockler David , Wilson Fiona , Kelly Áine M. 
Published: November 2023

Introduction:
Management of midlife blood pressure and hypertension status may provide a window of intervention to mitigate cognitive decline with advancing age. The aim of this review was to investigate the relationship between midlife hypertension and cognition in midlife and later life.

Methods:
Online electronic databases were searched from their inception to May 2022. Studies assessing midlife (40–65 years) hypertension and cognition at mid and/or later-life were included. A random effects meta-analysis was deemed appropriate.

Results:
One hundred forty-nine studies across 26 countries were included. Qualitative synthesis found negative relationships between midlife hypertension and later life cognition in the domains of memory, executive function, and global cognition. Metanalytical evidence revealed midlife hypertension negatively impacts memory, executive function, and global cognition but had no observed effect on attention at midlife.

Discussion:
Hypertension at midlife has a significant negative impact on cognition in mid-life and later life, namely memory, executive function, and global cognition.
Effect of vault on predicting postoperative refractive error for posterior chamber phakic intraocular lens based on a machine learning model
Authors: Jiang Yinjie , Shen Yang , Wang Lin , Chen Xun , Tang Jing , Liu Liu , Ma Tong , Ju Lie , Chen Yuzhong , Ge Zongyuan , Zhou Xingtao , Wang Xiaoying 
Published: November 2023

Purpose:
To investigate how vault and other biometric variations affect postoperative refractive error of implantable collamer lenses (ICLs) by integrating artificial intelligence and modified vergence formula.

Setting:
Eye and ENT Hospital of Fudan University, Shanghai, China.

Design:
Artificial intelligence and big data-based prediction model.

Methods:
2845 eyes that underwent uneventful spherical ICL or toric ICL implantation and with manifest refraction results 1 month postoperatively were included. 1 eye of each patient was randomly included. Random forest was used to calculate the postoperative sphere, cylinder, and spherical equivalent by inputting variable ocular parameters. The influence of predicted vault and modified Holladay formula on predicting postoperative refractive error was analyzed. Subgroup analysis of ideal vault (0.25 to 0.75 mm) and extreme vault (<0.25 mm or >0.75 mm) was performed.

Results:
In the test set of both ICLs, all the random forest-based models significantly improved the accuracy of predicting postoperative sphere compared with the Online Calculation & Ordering System calculator (P < .001). For ideal vault, the combination of modified Holladay formula in spherical ICL exhibited highest accuracy (R = 0.606). For extreme vault, the combination of predicted vault in spherical ICL enhanced R values (R = 0.864). The combination of predicted vault and modified Holladay formula was most optimal for toric ICL in all ranges of vault (ideal vault: R = 0.516, extreme vault: R = 0.334).

Conclusions:
The random forest-based calculator, considering vault and variable ocular parameters, illustrated superiority over the existing calculator on the study datasets. Choosing an appropriate lens size to control the vault within the ideal range was helpful to avoid refractive surprises.
Immersive virtual reality simulation training for cesarean section: a randomized controlled trial
Authors: Kim Hyeon Ji , Lee Hee Kyeong , Jang Ji Yeon , Lee Kyong-No , Suh Dong Hoon , Kong Hyoun-Joong , Lee Seung-Hee , Park Jee Yoon 
Published: November 2023

Background:
Cesarean section (CS) is a complex surgical procedure that involves many steps and requires careful precision. Virtual reality (VR) simulation has emerged as a promising tool for medical education and training, providing a realistic and immersive environment for learners to practice clinical skills and decision-making. This study aimed to evaluate the educational effectiveness of a VR simulation program in training the management of patients with premature rupture of membranes (PROM) and CS.

Materials and Methods:
A two-arm parallel randomized controlled trial was conducted with 105 eligible participants randomly assigned to the VR group (n=53) or the control group (n=52) in a 1:1 ratio. The VR group received VR simulation training focused on PROM management and CS practice, while the control group watched a video presentation with narrative of clinical scenario and recording of CS. Both groups completed questionnaires assessing their prior experiences with VR, experience in managing patients with PROM and performing CS, as well as their confidence levels. These questionnaires were administered before and after the intervention, along with a mini-test quiz.

Results:
Baseline characteristics and previous experiences were comparable between the two groups. After the intervention, the VR group had higher confidence scores in all four aspects, including managing patients with PROM, performing CS as an operator, and understanding the indications and complications of CS, compared to the control group. The VR group also achieved significantly higher scores on the mini-test quiz [median (interquartile range), 42 (37–48) in the VR group; 36 (32–40) in the control group, P<0.001].

Conclusion:
VR simulation program can be an effective educational tool for improving participants’ knowledge and confidence in managing patients with PROM and performing CS.
A decision tree model to help treatment decision-making for severe spontaneous intracerebral hemorrhage
Authors: Wang Kaiwen , Liu Qingyuan , Mo Shaohua , Zheng Kaige , Li Xiong , Li Jiangan , Chen Shanwen , Tong Xianzeng , Cao Yong , Li Zhi , Wu Jun , Wang Shuo 
Published: November 2023

Background:
Surgical treatment demonstrated a reduction in mortality among patients suffering from severe spontaneous intracerebral hemorrhage (SSICH). However, which SSICH patients could benefit from surgical treatment was unclear. This study aimed to establish and validate a decision tree (DT) model to help determine which SSICH patients could benefit from surgical treatment.

Materials and methods:
SSICH patients from a prospective, multicenter cohort study were analyzed retrospectively. The primary outcome was the incidence of neurological poor outcome (modified Rankin scale as 4-6) on the 180th day post-hemorrhage. Then, surgically-treated SSICH patients were set as the derivation cohort (from a referring hospital) and validation cohort (from multiple hospitals). A DT model to evaluate the risk of 180-day poor outcome was developed within the derivation cohort and validated within the validation cohort. The performance of clinicians in identifying patients with poor outcome before and after the help of the DT model was compared using the area under curve (AUC).

Results:
1260 SSICH patients were included in this study (middle age as 56, and 984 male patients). Surgically-treated patients had a lower incidence of 180-day poor outcome compared to conservatively-treated patients (147/794 vs. 128/466, P<0.001). Based on 794 surgically-treated patients, multivariate logistic analysis revealed the ischemic cerebro-cardiovascular disease history, renal dysfunction, dual antiplatelet therapy, hematoma volume, and Glasgow coma score at admission as poor outcome factors. The DT model, incorporating these above factors, was highly predictive of 180-day poor outcome within the derivation cohort (AUC, 0.94) and validation cohort (AUC, 0.92). Within 794 surgically-treated patients, the DT improved junior clinicians’ performance to identify patients at risk for poor outcomes (AUC from 0.81 to 0.89, P<0.001).

Conclusions:
This study provided a DT model for predicting the poor outcome of SSICH patients post-surgically, which may serve as a useful tool assisting clinicians in treatment decision-making for SSICH.
Predictive models in chronic kidney disease: essential tools in clinical practice
Authors: Spasiano Andrea , Benedetti Claudia , Gambaro Giovanni , Ferraro Pietro Manuel 
Published: November 2023

Purpose of review
The integration of risk prediction in managing chronic kidney disease (CKD) is universally considered a key point of routine clinical practice to guide time-sensitive choices, such as dialysis access planning or counseling on kidney transplant options. Several prognostic models have been developed and validated to provide individualized evaluation of kidney failure risk in CKD patients. This review aims to analyze the current evidence on existing predictive models and evaluate the different advantages and disadvantages of these tools.

Recent findings
Since Tangri et al. introduced the Kidney Failure Risk Equation in 2011, the nephrological scientific community focused its interest in enhancing available algorithms and finding new prognostic equations. Although current models can predict kidney failure with high discrimination, different questions remain unsolved. Thus, this field is open to new possibilities and discoveries.

Summary
Accurately informing patients of their prognoses can result in tailored therapy with important clinical and psychological implications. Over the last 5 years, the number of disease-modifying therapeutic options has considerably increased, providing possibilities to not only prevent the kidney failure onset in patients with advanced CKD but also delay progression from early stages in at-risk individuals.
Adrenal Suppression in Children During the Treatment for Acute Lymphoblastic Leukemia Beyond Induction
Authors: Fortin-Boudreault Renée-Pier , Ahmet Alexandra , Lawrence Sarah , Schlosser Mary-Pat , Bassal Mylène 
Published: November 2023

Background:
Adrenal suppression (AS) is an iatrogenic, life-threatening condition that can occur after glucocorticoid exposure. Despite recognition that AS occurs after induction phase treatment in children with acute lymphoblastic leukemia (ALL), the risk of AS in phases beyond induction is unknown. We conducted a pilot study in pediatric patients with ALL to ascertain whether the risk of AS persists in post-induction phases of treatment.

Procedure:
Patients diagnosed between 12 months to younger than 18 years with B or T-ALL and starting any new phase of treatment were eligible for the study. Relapsed or infant ALL were excluded. Low dose ACTH stimulation testing (LDST), measurement of albumin and cortisol binding globulin were performed in all patients. Screening for symptoms of AS was done.

Results:
Twenty-four patients enrolled in the study. One was diagnosed with clear AS. Five others had a borderline cortisol peak, representing possible mild AS. Symptoms were nonspecific and did not help distinguish patients with normal LDST from those with borderline or abnormal results.

Conclusion:
Patients on treatment for ALL continue to be at risk of AS beyond induction treatment. Although this risk appears small, physicians must be vigilant as patients may be asymptomatic but could develop adrenal crisis during treatment.
Nurses Job Crafting
Authors: Khattab Sabrein Mahmoud Ali Khalifa , Sorour Marwa Samir , Abdelaliem Sally Mohammed Farghaly 
Published: November 2023

AIM
The aim of this study was to investigate the relationship between nurses' job crafting, organizational identification, and work attachment.

BACKGROUND
Job crafting is a proactive activity in which nurses adapt the physical, cognitive, or social aspects of their jobs to make them more meaningful. Nurses are considered fundamental healthcare stakeholders who are able to transform initiatives implemented by the healthcare organization into valuable work outcomes. Nurses' perceptions of their jobs shape the community's opinion toward an organization.

METHODS
This is a descriptive correlational study. Two hundred seventy nurses were recruited from 1 governmental hospital in Egypt. Respondents completed the self-administered, printed questionnaires. Measures included job crafting, organizational identification, and work attachment questionnaires. Findings were investigated via descriptive and inferential statistics.

RESULTS
Over half of the nurses reported a moderate level of job crafting, whereas none of the nurses reported a high level of organizational identification. More than half of the nurses reported low levels of work attachment.

CONCLUSION
Job crafting is significantly related to nurses' organizational identification and work attachment.