bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macroalgal Biofilm Harbours a Wide Diversity of Parasitic Protists With Distinct Temporal Dynamics
Authors: Alacid Elisabet , Reñé Albert , Timoneda Natàlia , Garcés Esther 
Published: February 2025

ABSTRACTMarine macroalgae surfaces create a nutrient‐rich environment that promotes the formation of epiphyte biofilms. Biofilms are complex systems that facilitate ecological interactions within the community, yet parasitism remains largely unexplored. This study describes the diversity and temporal dynamics of the microeukaryotic community in the biofilm of Mediterranean macroalgae during summer, focusing on parasitic groups. Protist diversity was assessed using metabarcoding sequencing of the V4 region of the 18S rDNA gene using primers biased against metazoans. The macroalgal biofilm exhibited dynamic shifts in the microeukaryotic community structure associated to three phases of biofilm formation. Each phase was characterised by the dominance of specific eukaryotic and parasitic groups with clear successions between them. Our study revealed a high diversity of parasitic protists from different lineages in the macroalgal biofilm. These parasites can infect a wide variety of hosts, including the basibiont, species within the biofilm (micro‐ and macrocolonizers), nearby marine hosts and terrestrial organisms. The highest diversity and abundance of parasites were found in the mature phase of the biofilm, where the complexity and stability of the system seem to favour parasitism. The parasite assemblage was dominated by Apicomplexa, with many corresponding to unknown diversity, demonstrating that biofilms are a hotspot of unknown parasitic interactions. These parasites could potentially affect the dynamics of these communities and facilitate ecological interactions between the biofilm and surrounding organisms, suggesting that parasitism play a key, but still unexplored role, in shaping complex marine biofilms network.
Automated corrosion surface quantification in steel transmission towers using UAV photogrammetry and deep convolutional neural networks
Authors: Savino Pierclaudio , Graglia Fabio , Scozza Gabriele , Di Pietra Vincenzo 
Published: February 2025

AbstractCorrosion in steel transmission towers poses a challenge to structural integrity and safety, requiring efficient detection methods. Traditional visual inspections are unsustainable due to the complexity and volume of structures. Their manual, qualitative, and subjective nature often leads to inconsistencies in maintenance planning. This study proposes a deep learning‐based approach for semantic segmentation of corroded areas on steel towers. Using the DeepLabv3+ model, the network was trained and validated on 999 field photographs. MobileNetV2, serving as the feature extractor, was chosen for its optimal balance between accuracy and computational efficiency, achieving a validation accuracy of 90.8% and a loss of 0.23. The trained network was applied to real‐world inspections using orthomosaics derived from photogrammetric reconstructions of the South‐East tower at the Torino Eremo broadcasting center. These photogrammetric products not only enabled precise segmentation of corroded areas but also provided the foundation for corrosion quantification with metrical accuracy, a critical advantage for maintenance planning. Unlike traditional image segmentation methods, which lack a spatial reference and precise scaling, the photogrammetric approach ensures that the corrosion extent and distribution are quantified in exact physical dimensions, enhancing the reliability of the analysis. The results show that deep learning‐based inspections can automate detection, providing reliable data and reducing reliance on manual inspections, enhancing efficiency, safety, and accuracy.
Can Ambivalent Heritage Embody a Nation? The Meaning‐Making of Japanese Colonial Legacies in Contemporary Taiwan
Authors: Yao Ming‐li 
Published: February 2025

ABSTRACTThis study explores how state and nonstate actors endorsed and utilised heritage from the Japanese colonial era to redevelop the cultural discourse supporting Taiwanese nationalism. Grounded in the methodological approach to social constructionism, this study examines how these actors created and recreated the meanings of ‘ambivalent’ heritage places to embody Taiwan as one ‘imagined community’. The results suggest that the state invented and reinvented the meaning of colonial‐era heritage to be associated with the political discourse of multiculturalism, social evolution and political modernisation, reinforcing its legitimacy. Following and even transcending the official narrative, nonstate actors elicited a meaning of the colonial‐era heritage as intertextually associated with rituals, happy memories, everyday environment and individual experiences, revealing who served as the nationalists reconstructing the ‘national’ culture in a social context.
Portion Estimation, Satiety Perception and Energy Intake Following Different Breakfast Portion Sizes in Healthy Adults
Authors: Kwiecien Kinga , Santos‐Merx Lourdes , Sahota Tarsem , Coulthard Helen , Da Boit Mariasole 
Published: February 2025

ABSTRACTExpected satiety is a key element in predicting meal portion size and food consumption; however, how this can be affected by different breakfast portion sizes is unknown. The study examined the impact of different breakfast portions on satiety, portion size, and energy intake and comprised an online survey and an experimental intervention. Sixteen adults (9 women, BMI: 24.9 ± 4.3 kg/m2) rated images of three portion sizes (small, standard, large) of the same breakfast using an ordinal scale. Subsequently, they were asked to self‐prepare and consume ad libitum the three breakfast portions in a randomised order on different days and to complete a food diary. Satiety and portion size perception were re‐measured upon consumption of each breakfast. For both the visual image and breakfast consumption, the small breakfast portion was rated as the smallest and least filling, while the large portion was rated as the largest and most filling (p < 0.05). When consuming the small breakfast, participants reported being hungrier and less full between breakfast and lunch (p < 0.05) and had a higher energy intake from lunch onward, due to more snacking (p < 0.05). However, the total daily energy intake was not different among the three breakfast portion sizes. Individuals seemed accustomed to predicting satiety and portion size from images. The consumption of the small breakfast was judged as not filling enough and was accompanied by a higher energy intake via energy‐dense snacks. Based on these preliminary findings, breakfast size reduction may lead to unhealthy compensatory energy intake by snacking on energy‐dense foods.
Paced Breathing Associated With Pupil Diameter Oscillations at the Same Rate and Reduced Lapses in Attention
Authors: Andrews Ralph , Melnychuk Michael , Moran Sarah , Walsh Teigan , Boylan Sophie , Dockree Paul 
Published: February 2025

ABSTRACTA dynamical systems model proposes that respiratory, locus coeruleus—noradrenaline (LC‐NA), and cortical attentional systems interact, producing emergent states of attention. We tested a prediction that fixing respiratory pace (versus spontaneous respiration) stabilizes oscillations in pupil diameter (LC‐NA proxy) and attentional state. Primary comparisons were between ‘Instructed Breath’ (IB) and ‘No Instructed Breath’ (NIB) groups. Secondarily, we investigated the effects of shifting respiratory frequency in the IB group from 0.15 to 0.1–0.15 Hz in Experiment 1 (n = 55) and 0.15–0.1 Hz only in Experiment 2 (n = 48) (replication). In the Paced Auditory Cue Entrainment (PACE) task, participants heard two auditory tones, alternating higher and lower pitches, cycling continuously. Tones acted as a breath guide for IB and an attention monitor for both groups. Participants gave rhythmic mouse responses to the transition points between tones (left for high‐to‐low, right for low‐to‐high). We derived accuracy of mouse click timing (RTm), variability in click timing (RTVL), and counts of erroneously inverting the left/right rhythm (IRs and Switches). Despite no differences between groups in RTm or RTVL, IB committed significantly fewer IRs and switches, indicating less lapses in attention during paced breathing. Differences in behavioral metrics were present across tone cycle frequencies but not exclusive to IB, so breath frequency did not appear to have a specific effect. Pupil diameter oscillations in IB closely tracked the frequency of the instructed breathing, implicating LC‐NA activity as being entrained by the breath intervention. We conclude that pacing respiratory frequency did stabilize attention, possibly through stabilizing fluctuations in LC‐NA.
Restoring Halodule uninervis: evaluating planting methods and biodiversity
Authors: Cossa Damboia , Cossa Minda , Nhaca Jeremias , Timba Ilário , Chunguane Yudmila , Vetina Alvaro , Macia Adriano , Gullström Martin , Infantes Eduardo 
Published: February 2025

Seagrass provides a crucial habitat for numerous marine species and serves as a vital food source for endangered species, like dugongs. While extensive research on restoration has been conducted on certain temperate and slow‐growing climax seagrass species, limited attention has been given to tropical pioneer species. This study aimed to assess and compare two restoration methods for the pioneer seagrass Halodule uninervis and evaluate their potential for biodiversity recovery after planting. We conducted a field experiment at subtropical Inhaca Island, southern Mozambique, testing the efficiency of two planting methods (plugs and single shoots) and two planting densities (~100 and ~300 shoots/m2). We monitored seagrass shoot density in two sites for 16 months, and benthic macrofauna density for 12 months. Results demonstrated that seagrass could grow in all combinations of planting methods and densities in both sites. Specifically, the single shoot method at the high‐density treatment proved the most effective, resulting in approximately 1000 shoots/m2 within a year. Faunal densities, primarily dominated by polychaetes followed by malacostraca, bivalves, and gastropods, indicated rapid colonization of the planted areas, especially in the high‐density treatments. Our findings suggest that restoring H. uninervis is feasible using the two tested planting methods. This is particularly significant because H. uninervis is a preferred dugong food source, and its decline due to anthropogenic activities could be reversed through restoration efforts. Nonetheless, conserving existing seagrass should be the primary focus, and restoration approaches should be employed as a valuable tool for managing coastal areas.
Rare‐Earth Mineralization in Dong Pao Deposit, Laichau Province, Vietnam
Authors: Bui Tinh Thanh , Watanabe Yasushi , Tupaz Carmela Alen J. , Echigo Takuya , Aoki Shogo , Hoshino Mihoko 
Published: February 2025

ABSTRACTThe Dong Pao deposit in northern Vietnam is associated with the Paleogene Dong Pao Syenite Complex, which intruded into the limestone of the Triassic age. Although the Dong Pao deposit has been attracting exploration for decades as a promising rare earth deposit, it has not been well studied in terms of mineralization style and formation process until recently. We describe the occurrence of the orebodies of the deposit and the mineralogy of collected samples to discuss the mineralization style and process that formed the huge rare earth resource at Dong Pao. The deposit is composed of 60 orebodies, which occur where the syenite of the Eocene Dong Pao Complex contacts with the limestone of the Dong Giao Formation. Most of the orebodies exhibit a lenticular shape, situated at the boundary between the syenite and limestone along the margin of the complex, with the exception of the F3 orebody, where carbonatite is located. The F3 carbonatite consists mainly of calcite, which has carbon and oxygen isotope ratios slightly to moderately deviated from primary igneous carbonatites, due possibly to the assimilation of limestone. Two distinct styles of rare earth mineralization, metasomatized carbonatite and metasomatized limestone, are observed. The F3 metasomatized carbonatite is characterized by the replacement of calcite by synchysite‐(Ce) and parisite‐(Ce) in addition to fluorite, barite, celestine, and sulfides. The metasomatic limestone orebodies contain dolomite overprinted by the assemblage of barite, fluorite, bastnäsite‐(Ce), parisite‐(Ce), quartz, and sulfides. The metasomatic limestone zone at the F9 orebody is intruded by hydrothermal veins, which contain similar mineral assemblage to the metasomtatic zone, although HREE‐enriched minerals such as xenotime and synchysite‐(Y) are present. The REE mineralization in these metasomatized rocks is interpreted to have formed by the reaction of carbonate‐rich metasomatic fluids with carbonatite at F3 and fluorine‐rich fluids at the other orebodies with limestone. Such different fluids possibly exsolved from carbonatite magma and syenite magma, respectively. The Dong Pao deposit is an analogue of skarn deposits, although instead of calc‐silicate minerals, the metasomatic rocks at Dong Pao contain fluorite, barite, and REE‐bearing flourcarbonate minerals, because of the scarcity of silica and alumina in the hydrothermal fluids and host rocks. A large tonnage of the resource (> 4.7 Mt. of REO) is estimated at Dong Pao because of many orebodies scattered around the complex. In addition, the present semi‐tropical climate of northern Vietnam accelerates surface weathering of orebodies, upgrading the REE ores.
Multi‐omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae
Authors: Barreda Léa , Brosse Céline , Boutet Stéphanie , Klewko Nicolas , De Vos Delphine , Francois Tracy , Collet Boris , Grain Damaris , Boulard Céline , Totozafy Jean Chrisologue , Bernay Benoît , Perreau François , Lepiniec Loïc , Rajjou Loïc , Corso Massimiliano 
Published: February 2025

SUMMARYSeeds of Brassicaceae produce a large diversity of beneficial and antinutritional specialized metabolites (SMs) that influence their quality and provide resistance to stresses. While SM distribution has been described in leaves and root tissues, limited information is available about their spatiotemporal accumulation in seeds. Camelina sativa (camelina) is an oilseed Brassicaceae cultivated for human and animal nutrition and for industrial uses. While we previously explored SM diversity and plasticity, no information is available about SM distribution and expression of related proteins and genes in camelina seeds. In this study, we used a multi‐omic approach, integrating untargeted metabolomics, proteomics, and transcriptomics to investigate the synthesis, modification, and degradation of SMs accumulated in camelina seed tissues (seed coat, endosperm, embryo) at six developmental and two germination stages. Metabolomic results showed distinct patterns of SMs and their related pathways, highlighting significant contrasts in seed composition and spatial distribution for the defense‐related and antinutritional glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana, and Brassica napus, three closely related Brassicaceae species. Notably, thanks to metabolomic and proteomic/transcriptomic techniques the variation in GSL spatial distributions was primarily driven by differences in their structure (metabolomics data) and transport (transcriptomic and proteomic data) mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly accumulated in the seed coat and endosperm, while mid‐ and short‐chain C3–C7 methylsulfinylalkyl GSLs were accumulated in the embryo. Characterizing the spatial dynamics of seed SMs provides valuable insights that can guide the development of crops with optimized distribution of beneficial and toxic metabolites, improving seed nutritional profiles.
Comparison of whole blood versus red blood cells and plasma to correct trauma‐induced coagulopathy ex vivo
Authors: Rossetto Andrea , Vulliamy Paul , Huish Sian , Cardigan Rebecca , Green Laura , Davenport Ross 
Published: February 2025

AbstractBackgroundEarly resuscitation is based on platelet‐poor components such as red blood cells and plasma (RBC + P), contributing to platelet dilution and worsening of trauma‐induced coagulopathy (TIC). We aimed to compare the ability of cold‐stored whole blood (WB) versus RBC + P as a single component to correct TIC.Study Design and MethodsBlood samples were collected on admission from trauma patients who required activation of the major hemorrhage protocol at a single UK major trauma center in 2021/2022. Samples were spiked ex vivo with volumes equivalent to two, four, or eight units of WB or RBC + P stored for a maximum of 2 weeks. Thromboelastometry, platelet counting, and multiple electrode aggregometry (MEA) were performed.ResultsSamples from 20 adult trauma patients were analyzed. Median age was 32 years (27–42), 89% were male, 70% had platelet dysfunction (tissue factor–activated ROTEM [EXTEM]–tissue factor–activated ROTEM with cytochalasin D [FIBTEM] clot amplitude at 5 min [A5] ≤ 30 mm), 65% were coagulopathic (EXTEM A5 ≤ 40 mm), and 42% died. EXTEM–FIBTEM A5 was higher following spiking with WB than RBC + P (33 mm, 26–33, vs. 27 mm, 24–30, p < .001). WB‐spiking corrected platelet dysfunction in 2 patient samples out of 20, whereas RBC + P increased the frequency of platelet dysfunction (1/20 sample) and TIC (4/20 samples). RBC + P was associated with a dose‐dependent deterioration in rotational thromboelastometry (ROTEM) clot strength and dynamics, platelet count, and aggregation in response to multiple agonists compared with WB‐spiking, which maintained or partially corrected these abnormalities.ConclusionCompared with RBC + P, WB better preserves ex vivo platelet‐related ROTEM parameters, platelet count, and aggregation, but does not fully correct these common derangements of TIC.
The Use of CT Scan as a Diagnostic Tool in Pet Rabbits Presenting With Signs of Ophthalmic Disease
Authors: Goossens Lauren‐Tess , Boroffka Susanne A. E. B. , Gumpenberger Michaela , Schoemaker Nico J. , Slenter Inge J. M. , Sanchez Rick F. 
Published: February 2025

ABSTRACTPurposeTo describe the findings of computed tomography (CT) used to investigate signs of ophthalmic disease in pet rabbits.MethodsRetrospective review of records of patients with signs of ophthalmic disease that underwent CT scans in three referral centers between 2001 and 2022. Signalment, presenting ophthalmic and nonophthalmic complaints, CT findings, and restrain modality were recorded.ResultsThere were 85 rabbits representing 14 breeds including 28/85 (32.9%) crossbreed/unknown and 25/85 (29.4%) Dwarf rabbits. The top presenting ophthalmic complaints were ocular discharge (48/85, 56.5%), exophthalmos (34/85, 40%), and epiphora (14/85, 16.5%), while 38/85 (44.7%) rabbits also had nonophthalmic complaints. Anesthetic complications forced 1/85 (1.18%) CTs to be stopped. There were ophthalmic abnormalities in the remaining 81/84 (96.4%) CTs with exophthalmos and/or nasolacrimal duct disease affecting 76/81 (93.8%) rabbits and 68/81 (84%) also showing nonophthalmic abnormalities. Ophthalmic abnormalities were seen with nonophthalmic disease in 64/81 (79%) rabbits with dental disease being the most common (52/64, 81.3%) although only 20/52 (38.5%) had presented with dental complaints. No association was found between the presence of exophthalmos and/or NLD disease and dental disease. There were no obvious image artifacts in the 4/84 (4.8%) physically restrained patients.ConclusionExamination with CT in the present study mainly supported the investigation of ocular discharge, exophthalmos, and epiphora, and of possible local and systemic involvement in one eye with intraocular neoplasia. Adnexal disease was often seen with previously unknown, nonophthalmic pathology, which warrants prospective, controlled studies. Image quality studies in a large number of physically restrained patients are also warranted.
Efficacy of communication interventions for promoting blood donation in low‐ and middle‐income countries: A systematic review
Authors: Appiah Bernard , Asamoah‐Akuoko Lucy , Ahmed Md Koushik , Tancred Tara , Dei‐Adomakoh Yvonne , Singh Gursimar , Reilly Cavan , Olayemi Edeghonghon , Delaney Meghan , France Christopher R. , Benneh‐Akwasi Kuma Amma , Yawson Alfred Edwin , Telke Susan , Asiedu‐Danso Michelle , Dei Emmanuel Nene , Adongo Philip , Bates Imelda 
Published: February 2025

AbstractBackground and ObjectivesIn low‐ and middle‐income countries (LMICs), information on the efficacy of communication interventions promoting blood donation is very scarce. The present review aimed to identify specific communication interventions and their efficacy for increasing blood donation in LMICs.Materials and MethodsThe databases searched were PubMed, Scopus, PsycINFO, Web of Science, CINAHL, ProQuest, AJOL and CAB Abstracts. Grey literature sources included the websites of African Society of Blood Transfusion, International Society of Blood Transfusion and World Health Organization. The outcomes of interest were donation attempt or actual blood donations.ResultsA total of 16 studies including nine randomized controlled trials (RCTs) were included in the review. The communication interventions included social media and mass media, such as radio and television, as platforms for promoting blood donation, and newspaper articles or advertisements as an incentive for donor recruitment. Mobile text messages were used either alone or in combination with phone calls, and other interventions such as meetings and brochures. Only three of the 16 studies specifically targeted behavioural science theories, and none of the 16 studies used any implementation science framework. For some communication interventions, there was evidence of statistically significant increases in donations, but the quality of the studies was weak.ConclusionThe efficacy of communication interventions for promoting blood donations in LMICs remains limited due to few rigorous studies. More rigorous, theory‐based studies on the use of communication interventions to increase blood donation in LMICs, especially in sub‐Saharan Africa where no prior RCT were identified, are needed.
CaMKII at the crossroads: calcium dysregulation, and post‐translational modifications driving cell death
Authors: Mattiazzi Alicia , Jaquenod De Giusti Carolina , Valverde Carlos A. 
Published: February 2025

AbstractThe multifunctional Ca2+/calmodulin‐dependent protein kinase II (CaMKII) regulates numerous proteins involved in excitation–contraction–relaxation coupling and cardiac excitability. However, its overactivation induces severe Ca2+/handling alterations, playing a significant role in the pathogenesis of diseases such as hypertrophy, arrhythmias and cell death, which can ultimately lead to heart failure. Being a suitable target for various aberrant signals that characterize several diseases, such as Ca2+ overload, oxidative stress or excessive glycosylation, CaMKII shifts under these conditions from a physiological regulator to a pathological molecule. In this review, we explore the evolution of knowledge regarding the role of CaMKII activation on cell death across different pathological contexts, focusing on the converging mechanisms that transform the enzyme from an ally into a villain.
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Complete genome sequences of two verona integron-encoded metallo-ß-lactamase (VIM)-producing enterobacterales isolated from dogs in the United States
Authors: Desai Dhruv , Cole Stephen D. 
Published: February 2025

ABSTRACT

This announcement reports the complete genome sequences, created by combined Illumina and Oxford Nanopore sequencing, of two carbapenemase-producing Enterobacterales (
Escherichia coli
[LaAc-1–20] and
Enterobacter hormaechei
, strain 19632–21) isolated from dogs in the United States. Both isolates harbor a blaVIM-4 gene found on a 47 kb plasmid and on the bacterial chromosome, respectively.