bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peculiarity of autoimmune hepatitis triggered by SARS-CoV-2 infection
Authors: Gallo Antonella , Ibba Francesca , Massaro Maria Grazia , Rognoni Fiammetta , Giustiniani Maria Cristina , Ponziani Francesca Romana , Montalto Massimo 
Published: November 2023

Introduction: Recently, medical interest has been growing in SARS-CoV-2 infection and its multiorgan involvement, including the liver. Up until now, a few reports have described autoimmune hepatitis (AIH) triggered by SARS-CoV-2 infection, but no data are available about the specific liver inflammatory infiltrate and cluster of differentiation. We report a case of AIH triggered by SARS-CoV-2 infection, with a particular focus on its histological and mainly immunohistochemical features. Case description: A 60-year-old man, with a history of paucisymptomatic SARS-CoV-2 infection that occurred one month earlier, was admitted for alterations of hepatocellular necrosis and cholestasis indexes. He completed vaccination for SARS-CoV-2 a year earlier. The serologies for hepatotropic viruses were negative. The anti- smooth muscle antibodies (ASMA) and antinuclear antibodies (ANA) results were positive. Anti-liver kidney microsome (anti-LKM) antibodies and antimitochondrial (AMA) were negative. By liver biopsy, haematoxylin-eosin staining highlighted severe portal inflammation with a rich CD38+ plasma cell component, while immunohistochemical staining showed low cell CD4+ count and prevalence of CD8+ and CD3+. After biopsy, the patient started an immunosuppressant regimen, with benefit. Discussion: We can conclude that the patient developed a type 1 AIH triggered by SARS-CoV-2 infection. The presence of CD8 T-cells at immunohistochemical examination suggests different mechanisms from classic AIH. Similar cases are described after AIH triggered by SARS-CoV-2 vaccination. Conclusion: The AIH after SARS-CoV-2 infection developed by the patient showed a histological picture similar to a classic AIH for the abundant presence of plasma cells, and immunohistochemical features similar to those described after SARS-CoV-2-vaccination.
Is access to NHS tongue tie services equitable for mothers and babies in the UK?
Authors: Carter Elizabeth , Armstrong Louise , Kindred-Spalding Elaine , Gale Cathy , Wilson Deb 
Published: November 2023

Background/Aims NHS tongue tie service provision throughout the UK has previously been described as a ‘post code lottery’. A 2021 survey was conducted, with the aim to provide an update to a 2016 survey of tongue tie services in the UK. Methods A total of 179 trusts or health boards were approached with similar survey questions to a 2016 survey and achieved a response rate of 72% (n=130). Results There were similarities to the findings of the previous National Childbirth Trust survey, although there was an overall increase in NHS services provided. There is still variation in terms of the lead professional involved in delivering tongue tie services at the point of contact with the mother and baby, the criteria used for referral, the waiting time for assessment and treatment and the follow-up processes. Evidence supporting most aspects of care is limited, which causes much professional discussion. Conclusions The provision of NHS tongue tie services is still a ‘post code lottery’ and inequitable for mothers and babies.
Type 2 diabetes linked FTO gene variant rs8050136 is significantly associated with gravidity in gestational diabetes in a sample of Bangladeshi women: Meta-analysis and case-control study
Authors: Amin U. S. Mahzabin , Rahman Tahia Anan , Hasan Mashfiqul , Tofail Tania , Hasanat Muhammad Abul , Seraj Zeba I. , Salimullah Md 
Published: November 2023

Objective
Gestational diabetes mellitus (GDM) is a growing public health concern that has not been extensively studied. Numerous studies have indicated that a variant (rs8050136) of the fat mass-associated gene, FTO, is associated with both GDM and Type 2 diabetes mellitus(T2DM). We conducted a meta-analysis on the association between the FTO single nucleotide polymorphism (SNP) rs8050136 and T2DM, followed by a case-control study on the association of the said SNP and GDM in a sample of Bangladeshi women.

Method
A total of 25 studies were selected after exploring various databases and search engines, which were assessed using the Newcastle-Ottawa Scale (NOS). The MetaGenyo web tool was used to conduct this meta-analysis. A case-control study was performed on 218 GDM patients and 284 controls to observe any association between FTO rs8050136 and GDM. Genotyping was performed using the tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS) method, and statistical analyses were performed using various statistical softwares.

Results
In the meta-analysis 26231 cases and 43839 controls were examined. Pooled association analyses revealed a statistically significant relationship between the FTO rs8050136 polymorphism and an elevated risk of T2DM under all genetic models (P<0.05). In the case-control study, synergistic analyses of the SNP and gravida with GDM revealed a significant (P<0.01) association with an increase in odds by 1.6 to 2.4 folds in multigravida and decrease in odds by 2 folds in primigravida. A positive family history of diabetes and the minor allele of this SNP collectively increased the risk of developing GDM by many-fold (1.8 to 2.7 folds). However, after accounting for family history of diabetes and gravidity, analyses showed no significant association with GDM.

Conclusion
Our meta-analysis revealed a significant association between SNP rs8050136 of FTO with T2DM, and this variant was substantially associated with an increased risk of GDM in a sample of Bangladeshi multigravida women.
MORA and EnsembleTFpredictor: An ensemble approach to reveal functional transcription factor regulatory networks
Authors: Boyer Kevin , Li Louis , Li Tiandao , Zhang Bo , Zhao Guoyan 
Published: November 2023

Motivation
Our study aimed to identify biologically relevant transcription factors (TFs) that control the expression of a set of co-expressed or co-regulated genes.

Results
We developed a fully automated pipeline, Motif Over Representation Analysis (MORA), to detect enrichment of known TF binding motifs in any query sequences. MORA performed better than or comparable to five other TF-prediction tools as evaluated using hundreds of differentially expressed gene sets and ChIP-seq datasets derived from known TFs. Additionally, we developed EnsembleTFpredictor to harness the power of multiple TF-prediction tools to provide a list of functional TFs ranked by prediction confidence. When applied to the test datasets, EnsembleTFpredictor not only identified the target TF but also revealed many TFs known to cooperate with the target TF in the corresponding biological systems. MORA and EnsembleTFpredictor have been used in two publications, demonstrating their power in guiding experimental design and in revealing novel biological insights.
Unmasking hidden risks: The surprising link between PDE5 inhibitors and seizure susceptibility
Authors: Silva Alex Luiz Menezes da , Nascimento Chirlene Pinheiro , Azevedo Julianne Elba Cunha , Vieira Luana Rodrigues , Hamoy Akira Otake , Tiago Allan Carlos da Silva , Martins Rodrigues João Cleiton , de Araujo Daniella Bastos , Favacho Lopes Dielly Catrina , de Mello Vanessa Jóia , Hamoy Moisés 
Published: November 2023

Background
Phosphodiesterase 5 inhibitors (PDE5i) are the first line treatment for erectile dysfunction; however, several articles and case reports have shown central nervous system effects, that can cause seizures in susceptible patients. This study aims to describe the changes caused by the use of Sildenafil and Tadalafil through the analysis of abnormalities expressed in the electrocorticogram (ECoG) of rats and evaluate the seizure threshold response and treatment of seizures with anticonvulsants.

Materials and methods
The study used 108 rats (Wistar). Before surgery for electrode placement in dura mater, the animals were randomly separated into 3 experiments for electrocorticogram analysis. Experiment 1: ECoG response to using PD5i (Sildenafil 20mg/kg and Tadalafil 2.6mg/kg p.o.). Experiment 2: ECoG response to the use of PD5i in association with Pentylenetetrazole (PTZ—30 mg/kg i.p.), a convulsive model. Experiment 3: ECoG response to anticonvulsant treatment (Phenytoin, Phenobarbital and Diazepam) of seizures induced by association IPDE5 + PTZ. All recordings were made thirty minutes after administration of the medication and analyzed for ten minutes, only once. We considered statistical significance level of *p<0.05, **p<0.01 and ***p < 0.001.

Results
After administration of Sildenafil and Tadalafil, there were increases in the power of recordings in the frequency bands in oscillations in alpha (p = 0.0920) and beta (p = 0.602) when compared to the control group (p<0.001). After the use of Sildenafil and Tadalafil associated with PTZ, greater potency was observed in the recordings during seizures (p<0.001), however, the Sildenafil group showed greater potency when compared to Tadalafil (p<0.05). Phenobarbital and Diazepam showed a better response in controlling discharges triggered by the association between proconvulsant drugs.

Conclusions
PDE5i altered the ECoG recordings in the rats’ motor cortexes, demonstrating cerebral asynchrony and potentiating the action of PTZ. These findings demonstrate that PDE5i can lower the seizure threshold.
A novel, benchtop model for quantitative analysis of resistance in ventricular catheters
Authors: Gopalakrishnan Pranav , Faryami Ahmad , Harris Carolyn A. 
Published: November 2023

Introduction
The mechanisms of catheter obstruction are still poorly understood, but the literature suggests that resistance to fluid flow plays a significant role. We developed and assessed a gravity-driven device that measures flow through ventricular catheters. We used this device to quantitatively analyze the resistances of unused ventricular catheters used in the treatment of hydrocephalus; failed hydrocephalus catheters from our catheter biorepository were also evaluated quantitatively.

Methods
Catheters of three manufacturing companies were inserted into the benchtop model, which records time, flow rate, and pressure data using sensors. The relative resistances of catheters across six design models were evaluated. Experiments were performed to evaluate changes in the relative resistance of a catheter when the catheter’s holes were progressively closed. The relative resistance of explanted catheters from our catheter biorepository was also measured.

Results
Experimental results showed significant differences (P<0.05) between the relative resistances of different catheter models just after being removed from their packaging. A non-linear trend of increasing resistance was observed in experiments on catheters with artificially obstructed holes. Data from five individual benchtop models were compared, and the differences in measured data between the models were found to be negligible. A significant increase (P < 0.05) in relative resistance was observed in explanted catheters.

Conclusion
The current study sought to propose a novel in-vitro model and use it to examine data on differences in relative resistance among catheter models. From these experiments, we can rapidly correlate clinical patient cohorts to identify mechanisms of luminal shunt obstruction.
The effect of experiential learning interventions on physical activity outcomes in children: A systematic review
Authors: Varman Sumantla D. , Jones Rachel A. , Kelly Bridget , Hammersley Megan L. , Parrish Anne-Maree , Stanley Rebecca , Cliff Dylan P. 
Published: November 2023

Background
This systematic review examined the effectiveness of experiential learning interventions for improving children’s physical activity knowledge, attitudes, and behaviours. It also aimed to identify intervention characteristics that resulted in the greatest impact.

Methods
Four databases: Education Research Complete, Scopus, Web of Science and PsychINFO were searched from database inception to January 2023. Eligible studies: (1) included children 0–12 years; (2) assessed the effect of physical activity outcomes on children’s physical activity knowledge, attitudes or behaviour and (3) were randomised controlled trials conducted in any setting. Study risk of bias was assessed by two independent reviewers using the Cochrane risk of bias tool. Intervention approaches were categorised, and effect sizes were compared across studies for each outcome.

Results
Twelve studies were included in the review: ten in school age and two in below five years. For behavioural outcomes, six of eight studies showed medium to large effects (effects size (ES) range: 0.3–0.9), two of the three studies that assessed attitudinal outcomes displayed medium effects (ES range: 0.4–0.5) and both studies that assessed knowledge outcomes displayed medium to large effects (ES range: 0.4–1.3). The two experiential learning interventions among children < 5 years demonstrated small to medium effects on behaviour change (ES range: 0.2–0.5). Effective interventions combined enjoyable practical activities (fitness activities, games and challenges), with behaviour change techniques (goal setting, and self-monitoring), were underpinned by a behaviour change theory, and were often of short duration (< 4 months) but intense (several sessions/week). Moderate to high statistical heterogeneity was observed for behaviour outcomes and risk of bias across studies was generally high.

Conclusions
This review provides some evidence supporting the effectiveness of experiential learning interventions in improving physical activity outcomes in school-aged children. Additional evidence is needed in children <5 years old. Future experiential learning interventions need to strengthen the evidence with rigorous methodological quality and clear reporting of the experiential learning components.
Barriers and facilitators for interventions to improve ART adherence in Sub-Saharan African countries: A systematic review and meta-analysis
Authors: Buh Amos , Deonandan Raywat , Gomes James , Krentel Alison , Oladimeji Olanrewaju , Yaya Sanni 
Published: November 2023

Background
The HIV/AIDS pandemic remains a significant public health issue, with sub-Saharan Africa (SSA) at its epicentre. Although antiretroviral therapy (ART) has been introduced to decrease new infections and deaths, SSA reports the highest incidence of HIV/AIDS, constituting two-thirds of the global new infections. This review aimed to elucidate the predominant barriers and facilitators influencing ART adherence and to identify effective strategies to enhance ART adherence across SSA.

Methods
A comprehensive review was conducted on studies examining barriers to ART adherence and interventions to boost adherence among HIV-positive adults aged 15 and above in SSA, published from January 2010 onwards. The research utilized databases like Medline Ovid, CINAHL, Embase, and Scopus. Included were experimental and quasi-experimental studies, randomized and non-randomized controlled trials, comparative before and after studies, and observational studies such as cross-sectional, cohort, prospective and retrospective studies. Two independent reviewers screened the articles, extracted pertinent data, and evaluated the studies’ methodological integrity using Joanna Briggs Institute’s standardized appraisal tools. The compiled data underwent both meta-analysis and narrative synthesis.

Results
From an initial pool of 12,538 papers, 45 were selected (30 for narrative synthesis and 15 for meta-analysis). The identified barriers and facilitators to ART adherence were categorized into seven principal factors: patient-related, health system-related, medication-related, stigma, poor mental health, socioeconomic and socio-cultural-related factors. Noteworthy interventions enhancing ART adherence encompassed counselling, incentives, mobile phone short message service (SMS), peer delivered behavioural intervention, community ART delivery intervention, electronic adherence service monitoring device, lay health worker lead group intervention and food assistance. The meta-analysis revealed a statistically significant difference in ART adherence between the intervention and control groups (pooled OR = 1.56, 95%CI:1.35–1.80, p = <0.01), with evidence of low none statistically significant heterogeneity between studies (I2 = 0%, p = 0.49).

Conclusion
ART adherence in SSA is influenced by seven key factors. Multiple interventions, either standalone or combined, have shown effectiveness in enhancing ART adherence. To optimize ART’s impact and mitigate HIV’s prevalence in SSA, stakeholders must consider these barriers, facilitators, and interventions when formulating policies or treatment modalities. For sustained positive ART outcomes, future research should target specific underrepresented groups like HIV-infected children, adolescents, and pregnant women in SSA to further delve into the barriers, facilitators and interventions promoting ART adherence.
THE COMPOSITION OF THE ORGANIC MATTER AND MICROELEMENTS OF SPHAGNUM PEATS IN THE NORTHERN PART OF THE VASYUGAN PEAT DEPOSIT AND DIRECTIONS FOR THEIR USE
Authors: Maslov Stanislav Grigor'yevich , Inisheva Lidiya Ivanovna , Porokhina Ekaterina Vladimirovna 
Published: November 2023

The results of studies of sphagnum peats in the northern part of the Vasyugan peat deposit are presented. Peat sampling points were planned based on the materials of detailed exploration. The samples were collected in the form of mixed samples, which were taken at 3–4 points from the middle of the genetic layer of the peat deposit, the core height was 0.5 m. The peat quality assessment was carried out on the requirements for peat as a raw material for different uses. It is shown that the ash of different types of sphagnum peats may have elevated values of Ca, Mg and Fe. It is revealed that the elemental analysis of sphagnum peats has individual features of their content. It was found that the group composition of the organic matter of sphagnum peats is characterized by a high content of water-soluble and easily hydrolyzed matters and a low content of humic and fulvic acids. At the same time, in two samples, an increased content of bitumen was noted (samples 2 and 11), and in sample 9, a high content of humic and fulvic acids was detected and at the same time a low content of water-soluble and easily hydrolyzed matters. It is revealed that it is impossible to reduce all the variety of peat properties only to the species characteristic and the degree of decomposition due to the wide variability of the leading and subordinate peat-forming agents in the peat composition. It is shown that the properties of sphagnum peats of the oligotrophic type of the studied territory show their suitability for the production of many types of products, and the duration of operation of these reserves stretches up to 200 thousand years.
ANTIMICROBIAL ACTIVITY OF BUTANOL COMPLEXES AND ISOLATED SECONDARY METABOLITES OF SOME SPECIES OF GENUS SILENE OF THE FAMILY CARYOPHYLLACEAE
Authors: Zibareva Larisa Nikolayevna , Filonenko Elena Sergeevna , Andreeva Irina Sergeevna , Solovyanova Nadezhda Alekseevna , Nesterova Svetlana Vladimirovna 
Published: November 2023

Extracts of 9 species of the Caryophyllaceae family were screened for antimicrobial activity on the example of 17 types of strains of microorganisms. The inhibitory activity of 7 butanol extracts has been shown against a number of pathogenic strains belonging to the species Mycobacterium smegmatis, Phroteus mirabilis, Serratia marcescens, Staphylococcus aureus, Streptococcus faecalis. The extracts studied were most effective on the bacteria Bacillus cereus and Bacillus subtilis. It was found that all the studied plant species of the genera Lychnis and Silene contain ecdysteroids and flavonoids. The isolated individual compounds contained in all the studied species showed antimicrobial action on strains of a number of bacteria: the ecdysteroid 20-hydroxyecdysone suppressed the growth of the strain of R. mirabilis, while the flavonoid shaftoside inhibited strains of bacteria S. aureus, B. cereus, B. subtilis, R. mirabilis, M. smegmatis. Gram-negative bacteria Salmonella typhimurium, Klebsiella pneumoniae, Shigella sonnei, Pseudomonas aeruginosa are not susceptible to the antimicrobial action of extracts under experimental conditions.
Determination of antimicrobial activity was carried out by methods – diffusion and joint incubation of plant extracts and cells of test strains of microorganisms in a liquid nutrient medium LB at the ratio sample : medium 1 : 2 and 1 : 1. Increase in the concentration of secondary metabolites of ecdysteroids and flavonoids in experiments with butanol complexes of substances with a sample ratio the 1 : 1 environment led to an increase in antimicrobial activity up to the complete inhibition of Gram-positive bacteria B. subtilis and B. cereus by extracts of plant species Silene graefferi, S. colpophylla, S. sendtneri, S. linicola, S. jenisseensis S. viridiflora (seeds) and individual shaftoside.
Skilled in Creating Power Products for Electronic Instruments Size E Guitar String (Star Missiles) Through Project Based Learning Models
Authors: Murdhiastuti Henny 
Published: November 2023

This research was conducted in mathematics and sciences class XII-2 high school 1 Sigaluh, on electromagnetic induction material. The data of this research are data improving science process skills and scientific attitudes. The research instruments are in the form of observation sheets for project implementation assessments and questionnaires. The results of the questionnaire show that 63% of respondents use electricity-saving lamps, 31% have not used electricity-saving lamps, and 6% choose to be absent. The results of observation on the star missile PjBL showed an average of 81 categories of the high predicate. The results of the science teacher's response questionnaire obtained a score with the predicate category that strongly agrees with the star missile project-based learning. While the results of the cognitive assessment of the Star Missile PjBL have an average of 85, project appraisal with an average of 83.8 for star missile product appraisal has an average score of 85.8. Based on the results, it can be concluded that the application of the star missile project-based learning model can improve science process skills and scientific attitudes and is expected to can provide input for Physics teachers in implementing the star missile project-based learning model on KD 4.5.
The Void Ab Initio Theory in Comparative Perspective: J Marshall, H Kelsen, and Beyond
Authors: Tassopoulos Ioannis A 
Published: November 2023

Abstract
The void ab initio theory is traditionally associated with the retroactive effect of the unconstitutionality of a statute, in diffused systems of judicial review; as opposed to the prospective effect of unconstitutionality, in Austria, the archetype of centralized judicial review. The paper argues that the void ab initio theory goes far beyond the time-factor of judicial review, having two complementary aspects: 1) The void ab initio theory is tied to a robust theory of negative liberty; and substantive criminal law is its privileged field of application. The void ab initio theory goes back to Marbury v Madison (1803). 2) The void ab initio theory was strongly criticized by Kelsen, whose criticism is accurate, when the theory is unduly extended to the unconstitutionality of laws curtailing positive rights of the welfare state, eg, salaries and pensions, as happened recently in Greece. The act which abolishes the unconstitutional statute retroactively, has ‘the character of a legislative act.’ Indeed, the void ab initio theory in the field of positive social rights encroaches on legislative competence. The paper cocludes that the void ab initio theory is a strong expression of the ethos of legality; The void ab initio is most adequate for (cases involving) legal sanctions, on legal grounds, over adjudicative facts.