bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intracanal removal and apical extrusion of filling material after retreatment using rotary or reciprocating instruments: A new approach using human cadavers
Authors: Monteiro Thamyres M. , Cortes‐Cid Victor O. , Marceliano‐Alves Marilia F. V. , Campello Andrea F. , Bastos Luan F. , Lopes Ricardo T. , Siqueira José F. , Alves Flávio R. F. 
Published: October 2023

AbstractAimThis study compared intracanal removal of filling as well as the frequency and volume of extruded material after retreatment with either HyFlex or Reciproc instruments in mandibular teeth from cadavers.MethodologyThe root canals of 14 pairs of contralateral single‐rooted teeth in mandibles of cadavers were instrumented with Reciproc R40 and filled using lateral compaction. The mandibles were scanned in a micro‐computed tomographic (micro‐CT) device before and after retreatment procedures. The contralateral teeth were assigned to two groups (n = 14) according to the retreatment protocol using either HyFlex or Reciproc instrument systems. In the HyFlex group, the HyFlex Remover instrument was worked 3 mm short of the working length (WL), followed by HyFlex CM 40.04 and 50.04 at the WL. In the Reciproc group, the R50 instrument was worked up at the coronal two thirds, followed by two more cycles until the WL was reached. Pre‐ and post‐operative micro‐CT images were analysed for extrusion and intracanal removal of filling material.ResultsAfter retreatment, extrusion of filling material occurred in 11 (78%) and 14 (100%) teeth from HyFlex and Reciproc groups respectively (p > .05). A similar volume of extruded material was observed after retreatment with both systems (p > .05). A significant decrease in the intracanal filling volume was verified after retreatment with both tested systems (p < .05). However, residual filling material was found in all root canals, regardless of the system. The amount of filling material removed (HyFlex = 80.8%; Reciproc = 65.9%) and the operation time was similar between systems (p > .05).ConclusionsA high frequency of filling material extrusion was observed after retreatment with the two systems in a cadaver model, with no significant difference between them. Both protocols obtained similar efficacy in filling material removal procedures, although none completely cleaned the canals.
Carbamazepine Induced Erythroderma with Systemic Lymphadenopathy
Authors: Okuyama Ryuhei , Ichinohasama Ryo , Tagami Hachiro 
Published: October 2023

AbstractWe describe a 13‐year‐old girl with carbamazepine (CBZ)‐induced erythroderma associated with marked generalized lymphadenopathy, a very rare form of CBZ hypersensitivity. The results of lymphocyte stimulation test (LST) and patch testing provided valuable evidence for distinguishing CBZ hypersensitivity from immunoblastic lymphadenopathy (IBL) and infectious mononucleosis (IM). An increased LST value was the only clue leading us to suspect a hypersensitivity reaction against CBZ at a peak stage of the skin eruption. In contrast, CBZ patch testing, which was negative at that time, became positive when the erythroderma regressed after discontinuation of CBZ. These findings suggest that LST can be a useful tool in diagnosing similar cases of hypersensitivity reactions to CBZ and other drugs, especially when patch test results are negative.
A Case of Disseminated Superficial Actinic Porokeratosis Subsequent to Renal Transplantation
Authors: Matsushita Shigeto , Kanekura Takuro , Kanzaki Tamotsu 
Published: October 2023

AbstractDisseminated superficial actinic porokeratosis is characterized by the development of numerous annular keratotic lesions on sun‐exposed areas, accompanied by pathological evidence of cornoid lamellae. We examined a case of disseminated superficial actinic porokeratosis in a 40‐year‐old male who had undergone renal transplantation and was being treated with immunosuppressants. Five years after surgery, he began to develop numerous eruptions. Some of these eruptions enlarged and developed over a second period of five years until he finally required hospitalization.
Dietary citric acid decreased the theront number of Cryptocaryon irritans (Ciliata) in seawater‐adapted tilapia (Oreochromis niloticus)
Authors: Yildiz Hijran Yavuzcan , Yilmaz Bilgenur Harmansa 
Published: October 2023

AbstractCryptocaryoniasis remains a major parasitic disease and economic challenge for marine aquaculture. Cryptocaryoniasis in marine fish is caused by Cryptocaryon irritans (Ciliata). A theront is a motile, free‐swimming stage in the life cycle of C. irritans, which is typically the infective stage that actively seeks out a host to initiate infection. Population density and growth rate of theronts were investigated in Nile tilapia, Oreochromis niloticus fed with citric acid‐supplemented feed. The experiment involved feeding three diets with graded levels of citric acid (0, control diet, 0.5, 1 and 1.5 g kg−1 diet), to seawater‐adapted Nile tilapia (O. niloticus) juveniles for 21 days. The results showed that citric acid in the fish feed had an impact on the theront number of C. irritans in a manner of dose‐dependent. In the experimental cohort administered a diet supplemented with 1.5 g kg−1 citric acid, the population density of theronts was observed to be significantly reduced, measured at 29 ± 3.34, as opposed to 473.34 ± 16.48 in the control group at the culmination of the experiment. The observed population growth rate of theronts was significantly higher in the control group than in the group administered the citric acid feed (p < .005). The growth rate (r d−1) was 0.12 in control, 0.05 in 0.5 g kg−1, 0.031 in 1 g kg−1, and − 0.031 in 1.5 g kg−1 citric acid‐supplemented groups. Fish growth and feed conversion ratio were not affected by the citric acid in the feed. In conclusion, the findings of this investigation provide a valuable addition to our understanding of the potential protective effects of citric acid supplementation for fish against the C. irritans parasite. This is evidenced by the observed reduction in theronts present in the water.
Facing Apocalypse: Climate Mobilities and the Cinematic Child
Authors: Buesa Andrés 
Published: October 2023

AbstractThis article engages with the representations and meanings of child figures within US films about environmentally induced displacement. At the intersection between film studies, childhood studies, and the emerging scholarship on climate mobilities (Boas et al., 2022), it explores the ways in which three contemporary apocalyptic films—The Road (2009), Take Shelter (2011), and Greenland (2020)— mediate the relationship between mobility and environmental collapse through child characters. It argues that the functions attached to the child in these films—those of seer, victim, and carrier of hope and futurity—work to depoliticize climate mobilities, obscuring the varied aspirations, sociopolitical factors, and power structures that shape mobility choices in the context of environmental threat. As imaginary projections of an upcoming climate collapse, these films provide fertile ground for an exploration of the cultural ideals underpinning the construction of child characters, and the influence these have in the articulation of climate mobilities.
Parentage influence on gene expression under acidification revealed through single‐embryo sequencing
Authors: Fung Cheuk Wang , Chau Kin Yung , Tong Daniel Chun Sang , Knox Claire , Tam Sindy Sing Ting , Tan Sin Yen , Loi Danson Shek Chun , Leung Ziuwin , Xu Ying , Lan Yi , Qian Pei‐Yuan , Chan Kit Yu Karen , Wu Angela Ruohao 
Published: October 2023

AbstractThe dissolution of anthropogenic carbon dioxide (CO2) in seawater has altered its carbonate chemistry in the process of ocean acidification (OA). OA affects the viability of marine species. In particular, calcifying organisms and their early planktonic larval stages are considered vulnerable. These organisms often utilize energy reserves for metabolism rather than growth and calcification as supported by bulk RNA‐sequencing (RNA‐seq) experiments. Yet, transcriptomic profiling of a bulk sample reflects the average gene expression of the population, neglecting the variations between individuals, which forms the basis for natural selection. Here, we used single‐embryo RNA‐seq on larval sea urchin Heliocidaris crassispina, which is a commercially and ecologically valuable species in East Asia, to document gene expression changes to OA at an individual and family level. Three paternal half‐sibs groups were fertilized and exposed to 3 pH conditions (ambient pH 8.0, 7.7 and 7.4) for 12 h prior to sequencing and oxygen consumption assay. The resulting transcriptomic profile of all embryos can be distinguished into four clusters, with differences in gene expressions that govern biomineralization, cell differentiation and patterning, as well as metabolism. While these responses were influenced by pH conditions, the male identities also had an effect. Specifically, a regression model and goodness of fit tests indicated a significant interaction between sire and pH on the probability of embryo membership in different clusters of gene expression. The single‐embryo RNA‐seq approach is promising in climate stressor research because not only does it highlight potential impacts before phenotypic changes were observed, but it also highlights variations between individuals and lineages, thus enabling a better determination of evolutionary potential.
Feasibility of laparoscopic stapled partial cystectomy in canine cadavers
Authors: Reyes Bianca C. , Scharf Valery F. 
Published: October 2023

AbstractObjectiveTo describe a technique for laparoscopic partial cystectomy and evaluate sealing of canine urinary bladder tissue with an endoscopic stapler.Study DesignProspective, cadaveric study.Sample PopulationEleven canine cadavers.MethodsLaparoscopic partial cystectomy was performed with an endoscopic gastrointestinal anastomosis (GIA) stapler. Leak testing and urodynamic evaluation was performed to measure intravesical pressure at initial leakage and catastrophic failure or the maximum pressure at which intravesical pressure plateaued. Surgical time, complications, and site of leakage were also recorded.ResultsLaparoscopic partial cystectomy was successfully performed in 10 of 11 cadavers. Median procedure time was 13.6 min (10.1–15.2 min). Median intravesical pressure at initial leakage was 31 mmHg (28–56 mmHg) with a median increase from initial pressure of 15 mmHg (11–37 mmHg). No catastrophic failure of the cystectomy site was observed during leak testing. Intravesical pressure plateaued at a median of 52 mmHg (39–73 mmHg), which was a median of 13 mmHg (8–23 mmHg) higher than intravesical pressure at initial leakage. The median total volume infused at the time of plateau of intravesical pressure was 89 mL (58–133 mL).ConclusionLaparoscopic stapled partial cystectomy was feasible in canine cadavers. Endoscopic GIA staplers appear to seal the canine urinary bladder with minimal leakage.Clinical SignificanceUse of an endoscopic GIA stapler may provide a minimally invasive option for partial cystectomy in dogs. Further evaluation for application to clinical cases is warranted.
Continuous Stereolithography 3D Printing of Multi-Network Hydrogels in Triply Periodic Minimal Structures With Tunable Mechanical Strength for Energy Absorption
Authors: Guo Zipeng , Yang Ruizhe , Liu Jun , Armstrong Jason , Zhao Ruogang , Zhou Chi 
Published: October 2023

Abstract
This work presents a fast additive manufacturing (AM) protocol for fabricating multi-network hydrogels. A gas-permeable PDMS (polydimethylsiloxane) film creates a polymerization-inhibition zone, enabling continuous stereolithography (SLA) 3D printing of hydrogels. The fabricated multi-bonding network integrates rigid covalent bonding and tough ionic bonding, allowing effective tuning of elastic modulus and strength for various loading conditions. The 3D-printed triply periodic minimal structures (TPMS) hydrogels exhibit high compressibility with up to 80% recoverable strain. Additionally, dried TPMS hydrogels display novel energy/impact absorption properties. By comparing uniform and gradient TPMS hydrogels, we analyze their energy/impact absorption capability of the 3D-printed specimens. We use finite element analysis (FEA) simulation studies to reveal the anisotropy and quasi-isotropy behavior of the TPMS structures, providing insights for designing and controlling TPMS structures for energy absorption. Our findings suggest that gradient TPMS hydrogels are preferable energy absorbers with potential applications in impact resistance and absorption.
Constraining the Feasible Design Space in Bayesian Optimization With User Feedback
Authors: Jetton Cole , Campbell Matthew , Hoyle Christopher 
Published: October 2023

Abstract
This paper develops a method to integrate user knowledge into the optimization process by simultaneously modelling feasible design space and optimizing an objective function. In engineering, feasible design space is a constraint similar to those in optimization problems. However, not all constraints can be explicitly written as mathematical functions. This includes manufacturing concerns, ergonomic issues, complex geometric considerations, or exploring material options for a particular application. There needs to be a way to integrate designer knowledge into the design process and, preferably, use that to guide an optimization problem. In this research, these constraints are modeled using classification surrogate models and incorporated with Bayesian optimization. By suggesting design options to a user and allowing them to box off areas of feasible and infeasible designs, the method models both the feasible design space and an objective function probability of new design targets that are more optimal and have a high probability of being feasible. This proposed method is first proven with test optimization problems to show viability then is extended to include user feedback. This paper shows that by allowing users to box off areas of feasible and infeasible designs, it can effectively guide the optimization process to a feasible solution.
Experimental Investigation of Local Heat Transfer Coefficient in a Plate Heat Exchanger Using a Thin Heat Transfer Surface
Authors: Hirokawa Tomoki , Yamasaki Ayarou , Kawanami Osamu 
Published: October 2023

Abstract
This paper presents an experimental investigation of local heat transfer characteristics of single-phase flow in a plate heat exchanger (PHE). The local heat transfer coefficient is evaluated using a test section with PHE geometry for measuring wall temperature distribution. The test section of 1.5 mm thickness is employed to consider the heat conduction effect of the heat transfer plate. The results indicated that the local heat transfer coefficient is influenced by the development of the thermal boundary layer along the flow direction and the maldistribution of water flows along both the direction perpendicular to the flow and the stacking direction. The harmonic mean heat transfer coefficient calculated by the measured local heat transfer coefficient agrees with the average heat transfer coefficient evaluated by the modified Wilson plot method within ±25% and within ±16% for the hot side and the cold side, respectively.
Use of a microgravity analog to explore the effects of simulated microgravity on the development of
Escherichia coli
K12 biofilms
Authors: Hicks Janelle , Topolski Collin , Chavez Alba A. , Castillo Hugo A. 
Published: October 2023

ABSTRACT

The rapid development of space technologies and the increase of human presence in space has brought the discussion of the effects of microgravity on cells into the undergraduate classroom. This paper proposes an idea to simulate microgravity on a bacterial culture, suitable for an introductory microbiology laboratory. For this purpose, we show the use of a 2D clinostat designed for microbial studies, along with traditional microbiology techniques such as optical density, plate counts, and biofilm biomass measurement to test the effect of simulated microgravity on the growth of
Escherichia coli
K12. This exercise aims to facilitate further discussions on the effects of microgravity on bacteria growth and communication, as well as the use of technology to simulate space and predict physiological changes in cells.
HTLV-1 biofilm polarization maintained by tetraspanin CD82 is required for efficient viral transmission
Authors: Arone Coline , Martial Samuel , Burlaud-Gaillard Julien , Thoulouze Maria-Isabel , Roingeard Philippe , Dutartre Hélène , Muriaux Delphine 
Published: October 2023

ABSTRACT
The human T-lymphotropic virus type 1 (HTLV-1) is an oncogenic retrovirus whose transmission relies primarily on cell-to-cell contacts as cell-free viruses are poorly infectious. Among the intercellular transmission routes described, HTLV-1 biofilms are adhesive structures polarized at the cell surface that confine virions in a protective environment, which is believed to promote their simultaneous delivery during infection. Here, we show that several tetraspanins are enriched in HTLV-1 biofilms and incorporated into the viral envelope. However, we report that only the tetraspanin CD82 interacts with HTLV-1 Gag proteins which initiates their polarization into viral biofilms. Also, we demonstrate that CD82 maintains HTLV-1 biofilm polarization and favors viral transmission, as its silencing induces a complete reorganization of viral clusters at the cell surface and reduces the ability of infected T-cells to transmit the virus. Our results highlight the crucial role of CD82 and its glycosylation state in the architectural organization of HTLV-1 biofilms and their subsequent transfer through intercellular contacts.

IMPORTANCE
In the early stages of infection, human T-lymphotropic virus type 1 (HTLV-1) dissemination within its host is believed to rely mostly on cell-to-cell contacts. Past studies unveiled a novel mechanism of HTLV-1 intercellular transmission based on the remodeling of the host-cell extracellular matrix and the generation of cell-surface viral assemblies whose structure, composition, and function resemble bacterial biofilms. These polarized aggregates of infectious virions, identified as viral biofilms, allow the bulk delivery of viruses to target cells and may help to protect virions from immune attacks. However, viral biofilms’ molecular and functional description is still in its infancy, although it is crucial to fully decipher retrovirus pathogenesis. Here, we explore the function of cellular tetraspanins (CD9, CD81, CD82) that we detect inside HTLV-1 particles within biofilms. Our results demonstrate specific roles for CD82 in the cell-surface distribution and intercellular transmission of HTLV-1 biofilms, which we document as two essential parameters for efficient viral transmission. At last, our findings indicate that N-glycosylation of cell-surface molecules, including CD82, is required for the polarization of HTLV-1 biofilms and for the efficient transmission of HTLV-1 between T-lymphocytes.
Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats
Authors: Dong Yue , Li Yixuan , Ge Meiling , Takatsu Tetsuya , Wang Zongling , Zhang Xuelei , Ding Dewen , Xu Qinzeng 
Published: October 2023

ABSTRACT

Gastrointestinal microorganisms play a crucial role in host survival and adaptation, but information on host-specific selection or environmental factors that shape the microbiome in natural populations is limited. In this study, we employed 16S rRNA gene amplicon sequencing to investigate the composition and predicted the functions of gut microbiota of two ophiuroid species (
Ophiura sarsii
and its subspecies
O. sarsii vadicola
) from cold-water habitats across three geographically distant sea areas in the Northern Pacific Ocean. We also explored the potential influence of the host and environment on the microbiota. The two ophiuroids possessed diverse microbial communities, with Proteobacteria being the most dominant phylum in all four groups.
Aliivibrio
was the predominant genus in
O. sarsii
from the Bering Sea, while
Candidatus
Hepatoplasma was the dominant genus in
O. sarsii
from the Japan Sea and
O. sarsii vadicola
from the Yellow Sea. Predicted bacterial functions indicated that most of the pathways with significant differences belonged to metabolism and genetic information processing. Notably, no significant difference was observed between the two coexisting ophiuroid species in the Japan Sea. The high similarity in microbial communities between
O. sarsii
from the Japan Sea and
O. sarsii vadicola
from the Yellow Sea may be attributed to their analogous ecological niche. The host and the environment jointly shape the composition of the gut microbial community. The presence of specific microorganisms supports the ecological success of ophiuroids across diverse environments, providing a foundation for host adaptation to cold-water habitats.

IMPORTANCE

Gastrointestinal microorganisms are critical to the survival and adaptation of hosts, and there are few studies on the differences and functions of gastrointestinal microbes in widely distributed species. This study investigated the gut microbes of two ophiuroid species (
Ophiura sarsii
and its subspecies
O. sarsii vadicola
) in cold-water habitats of the Northern Pacific Ocean. The results showed that a combination of host and environmental factors shapes the intestinal microbiota of ophiuroids. There was a high similarity in microbial communities between the two groups living in different regions, which may be related to their similar ecological niches. These microorganisms played a vital role in the ecological success of ophiuroids as the foundation for their adaptation to cold-water environments. This study revealed the complex relationship between hosts and their gut microbes, providing insights into the role they play in the adaptation and survival of marine species.
Supplemental Material: In situ U-Pb dating of carbonate veins in Cambrian shales constrains fluid flow and hydrocarbon evolution at the southeastern margin of the Upper Yangtze platform, southwestern China
Authors: Fan Qingqing , Liu Dadong , et al.  
Published: October 2023

<p>Carbonate U-Pb isotope data, rare earth element data, and carbon and oxygen isotope data. </p>
Salinity as a Factor Limiting the Potential Taxonomic Richness of Crustaceans in Ecosystems of Hypersaline Reservoirs around the World (Review)
Authors: Anufriieva E. V. , Shadrin N. V. 
Published: October 2023

Abstract
Crustaceans (Crustacea) are one of the most diverse and successful groups in the biosphere, having also mastered different extreme habitats (in addition to freshwater and marine). Based on our own data and >200 literary sources, the way in which the degree of environmental extremeness can limit a potential taxonomic richness of crustaceans has been analyzed using the example of hypersaline waters. It is demonstrated that, with an increase in salinity, the number of classes and orders of the subtype Crustacea decreases linearly; the number of genera and species decreases exponentially. With an increase in environment salinity, the contribution of Arthropoda species to a total species richness of animals in hypersaline waters increases from 49 to 100%, the contribution of Crustacea species to the total species richness of Arthropoda increases from 66 to 78%, and the contribution of Branchiopoda to the species richness of Crustacea increases from 19 to 71%. In hypersaline reservoirs of Crimea in the range from 35 to 120 g/L, salinity is not the main factor determining the species richness and composition of the fauna. A combination of all other factors (first and foremost biotic) plays a more important role, and salinity becomes a tough environmental filter only at higher values (>100–120 g/L).