bluuebunny/crossref_metadata_2025_split huggingface dataset:
MSI2 regulates NLK-mediated EMT and PI3K/AKT/mTOR pathway to promote pancreatic cancer progression
Authors: Huang Longping , Sun Jian , Ma Yuteng , Chen He , Tian Chen , Dong Ming 
Published: February 2024

Abstract

Background: The incidence of pancreatic cancer is increasing by years, and the 5-year survival rate is very low. Our team have revealed that Musashi2 (MSI2) could promote aggressive behaviors in pancreatic cancer by downregulating Numb and p53. MSI2 also facilitates EMT in pancreatic cancer induced by EGF through the ZEB1-ERK/MAPK signaling pathway. This study aims to further explore the molecular mechanisms of MSI2-regulated downstream pathways in pancreatic cancer.
Methods: In vitro and in vivo experiments were conducted to investigate the role and mechanism of MSI2 in promoting malignant behaviors of pancreatic cancer through regulation of NLK.
Results: Genes closely related to MSI2 were screened from the GEPIA and TCGA databases. We found that NLK showed the most significant changes in mRNA levels with consistent changes following MSI2 interference and overexpression. The high correlation between MSI2 and NLK was also observed at the protein level. Multivariate analysis revealed that both MSI2 and NLK were independent adverse indicators of survival in pancreatic cancer patients, as well as join together. In vitro, silencing or overexpressing NLK altered cell invasion and migration, by regulating EMT and the PI3K-AKT-mTOR pathway. Silencing MSI2 reduced protein expression in the EMT and PI3K-AKT-mTOR pathways, leading to decreased cell invasion and migration abilities, while these effects could be reversed by overexpression of NLK. In vivo, MSI2 silencing inhibited liver metastasis, which could be reversed by overexpressing NLK.
Conclusions: Our findings elucidate the regulatory mechanisms of the MSI2-NLK axis in modulating aggressive behaviors of pancreatic cancer cells, which providing new evidence for therapeutic strategies in pancreatic cancer.
Sexual Dysfunction and Its Impact on Quality of Life among Female Haematological Cancer Patients: A Cross- Sectional Study in Malaysia
Authors: Gan Gin-Gin , Ng Diana-Leh-Ching , Kerishnan Jesinda Pauline , Ng Chong-Guan 
Published: February 2024

Abstract
Background:
Sexual function (SD) and quality of life (QoL) are significantly impacted by cancer and its treatments. However, information on sexual function is scarce, particularly among female patients with underlying haematological malignancies in Asia. This study aims to determine the prevalence of SD in these patients, identify associated factors, and examine their effects on QoL.
Methods:
This cross-sectional study, conducted in Malaysia from 2014–2016, involved female patients with haematological malignancies who were on active treatment or had completed active treatment. Sociodemographic and clinical data were obtained through patients interviews and medical records. Sexual functions were assessed using the Female Sexual Function Index, psychological aspects with the Hospital Anxiety and Depression Scale (HADS), and health-related QoL using the European Organization for the Research and Treatment of Cancer Quality of Life Questionnaire Core 30 version 3.0 questionnaire. Data were analysed using SPSS software.
Results:
Of the 89 patients recruited, 82% reported SD. Significant associations were found between SD and menopause (OR, 5.7; 95% CI, 1.60-20.34; p = 0.007) and higher HADS-depression score (OR, 1.6; 95% CI, 1.14–2.21; p = 0.006). Patients with SD exhibited lower median scores in Global Health Status/ QoL (QL2) (66.7 [IQR 33.3] vs. 91.7 [IQR 33.3], p = 0.009), role function (83.3 [IQR 33.3] vs. 100 [IQR 16.7], p = 0.033), cognitive function (83.3 [IQR 25.0] vs. 100 [IQR 16.7]) and pain (33.3 [IQR 33.3] vs. 0 [IQR 16.7]). Weak positive correlations were observed between FSFI score and QL2 (Rs = 0.252, p = 0.018), and cognitive function (Rs = 0.237, p = 0.026), while a moderate positive correlation was found with role function (Rs = 0.315, p = 0.003).
Conclusions:
SD is highly prevalent in female patients with haematological cancers, significantly impacting QoL, and this needs to be addressed accordingly.
LINC00323 induced by hypoxia promote cartilage callus by interacting with FUS to regulate PDGFB expression
Authors: 黄 Jiang , Wang Ju yong , Sun Xiang Yao , An Shuai , Cao Guang Lei 
Published: February 2024

Abstract
Intermittent hypoxia has been reported to contribute beneficial effects on fracture healing depending on various factors like duration, frequency, and severity. Yet, little is known about the underlying molecular mechanism. Our previous study found that LINC00323 was up-regulated under hypoxic conditions, suggesting that it might play a final role in hypoxia-induced fracture repair. The present study is to investigate the osteogenic effect of LINC00323 in vitro and in vivo. Upregulation of LINC00323 enhanced the mineralization and activity ALP and increased the expression of osteogenic markers. Further analysis revealed that LINC00323 promoted PDGFB expression by binding FUS to regulate the growth and osteogenic differentiation of MC3T3-E1. Lentivirus mediated LINC00323 particles were injected into the fracture site of the tibia of mice, and fracture healing was evaluated by X-rays, micro-CT examination, biomechanical test and histological staining. Local injection of Lentivirus-LINC00323 increased bone mass, biomechanical strength and cartilage callus formation. These findings indicated that LINC00323 induced the differentiation of osteoblast-like cells via regulation of the expression of PDGFB, represents a theoretical basis to accelerate fracture healing.
Shuffle Attention-based CNN Network for Visual Place Recognition
Authors: YE MINYING , JIN Yongze , LIU Yu , SHANGGUAN Anqi 
Published: February 2024

Abstract
Robot visual place recognition is a research hotspot, and robot place recognition systems based on 3D point clouds are even more focused. In recent years, scan-context descriptor imagery has become a standard method for 3D point cloud positioning. Based on its system characteristics, we integrated it with the Shuffle Attention (SA) mechanism module to improve its system performance. And we tested it on the public database NCLT dataset (North Campus Long-Term Vision dataset), and our method has good place recognition results.
Synthesis and evaluation of chitosan/miR-125b nanoparticles for targeting Raf-1 and BMPR1b genes in MCF-7 breast cancer cells
Authors: Norouzi Raana , Abousalehi Neda , Afshar Akbar Safipour 
Published: February 2024

Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and play important roles in cancer development and progression. MiR-125b-5p is a miRNA that has been reported to have diverse and context-dependent effects on different cancer types and subtypes. In this study, we aimed to investigate the expression and function of miR-125b-5p in MCF-7 breast cancer cells and to explore the potential of using chitosan nanoparticles for miR-125b-5p delivery. We found that miR-125b-5p was downregulated in MCF-7 cells compared to normal mammary epithelial cells, and that its overexpression reduced the viability of MCF-7 cells by targeting Raf-1 and BMPR1b genes, which are involved in cell survival and proliferation. We also synthesized and characterized chitosan/miR-125b nanoparticles (CNPs) and evaluated their in vitro release profile and cellular uptake. We showed that CNPs enhanced the delivery and efficiency of miR-125b-5p, resulting in a more potent inhibition of Raf-1 and BMPR1b gene expression and a greater reduction of cell viability. Our results suggest that miR-125b-5p and CNPs have potential anti-tumor effects on human breast cancer cells by suppressing Raf-1 and BMPR1b gene expression. Our study provides a new insight into the role and mechanism of miR-125b-5p and its target genes in breast cancer, and demonstrates the feasibility and efficacy of using chitosan nanoparticles for miR-125b-5p delivery.
The Effects of Health Education on Acute Intermittent Porphyria Attacks : A Retrospective Cohort Study
Authors: Zhao Lanlan , Li Jie , Li Pei , Zhao Xin , Ma Ningzhen , Zhang Songyun 
Published: February 2024

Abstract
Background: Acute Intermittent Porphyria (AIP) is characterized by acute attacks of neurovisceral symptoms. Infection and negative emotions are factors that can trigger acute AIP attacks, thus avoiding these potential triggers can effectively reduce the risk of acute attacks. During the COVID-19 pandemic, online health education provides patients with a convenient and safe way to receive health education, thus effectively improving their quality of life（QoL）. We explored the effect of online health education on acute AIP attacks during the COVID-19 pandemic.
Methods：This study retrospectively included 47 patients with AIP who were infected with COVID-19 and completed a questionnaire from November 2022 to March 2023. These participants were divided into two groups based on whether they had received centralized health education during COVID-19. The primary outcomes were acute AIP attacks, AIP attack severity, and QoL. Secondary outcomes were negative emotions and positive coping styles.
Results: In comparison to the non-health education group, the health education group showed a significant decrease in the incidence of acute AIP attacks (P < .05), a reduction in the severity of AIP attacks (P < .05), and an improvement in QoL (P < .05). Additionally, the health education group had a significant decrease in negative emotions (P < 0.05) and a substantial increase in positive coping styles (P < 0.05).
Conclusions: Health education may enhance patients' cognition and coping skills, and reduce negative emotions, thereby reducing the occurrence and severity of acute AIP attacks, and improving the patients' QoL.
Hyper-enrichment of heavy rare earth elements in highly evolved granites through multiple hydrothermal mobilizations
Authors: Li Martin Yan Hei , Zhou Mei-Fu 
Published: February 2024

Abstract
Highly evolved granites can be important hosts of rare earth element (REE) resources, and more importantly, they commonly serve as the protolith for regolith-hosted REE deposits to form during weathering. Highly evolved granites in the Zudong pluton, South China, are extremely rich in the heavy (H)REE (up to 8000 ppm total HREE), and display significant REE fractionation. Moreover, the HREE enrichment is positively correlated with the degree of REE fractionation, indicating a unique process in preferentially enriching the HREE during the evolution of the granites. Multiple stages of hydrothermal re-mobilization of the REE can account for the HREE mineralization, and these are recorded in the texture and composition of the zircon. In these processes, fluctuations in the F activity of the fluid caused alternating dissolution-reprecipitation and continuous growth of the zircon. REE were repeatedly mobilized and enriched in the fluid to precipitate the major HREE mineral synchysite-(Y), and partially incorporated into the growth zone of zircon, while other elements were largely lost to the fluid during the extensive dissolution of the rock-forming minerals. LREE were also likely substantially mobilized in the late hydrothermal stage and lost through complexation with Cl, causing the significant LREE depletion and, thus REE fractionation. This process continuously enriched host granites in the HREE to a potentially economic grade, making them favorable protoliths for subsequent supergene regolith-hosted HREE deposits.
Tetrahedral aluminum in tourmaline from a spinel-pargasite-metamorphosed mafic-ultramafic rock
Authors: Vereshchagin Oleg S. , Gritsenko Yuliya D. , Vigasina Marina F. , Dedushenko Sergey K. , Gorelova Liudmila A. , Pautov Leonid A. , Agahanov Atali A. , Chernyshova Irina A. , Zolotarev Anatoly A. 
Published: February 2024

Abstract
Tourmaline is a widespread borosilicate mineral that is well known for its variable chemistry. Although a major amount of octahedral Al in tourmaline is commonplace, the occurrence of significant amounts of tetrahedral Al is relatively rare. This paper focuses on tourmaline from the collection of the A.E. Fersman Mineralogical Museum (Russia) originated from Italy with up to 25% of Si replaced by Al at the tetrahedral site. The tourmaline is characterized by optical and scanning electron microscopy, Raman spectroscopy, infrared spectroscopy, Mössbauer spectroscopy, energy-dispersive and wavelength-dispersive X-ray analysis, laser ablation inductively coupled plasma optical emission spectrometry and single-crystal X-ray diffraction. The studied tourmaline occurs as transparent dark blue crystals (with equant external morphology) up to 3 mm in size and forms veinlets cutting a (Mg,Al)-rich metamorphosed mafic-ultramafic rock (Mg &gt;&gt; Fe) composed of spinel, pargasite, clinochlore, phlogopite, and hydroxylapatite. The studied tourmaline meets the criteria defining magnesio-lucchesiite and can be compositionally formed via Tschermak-like ([6]Me2+ + [4]Si4+ ↔ [6]Al3+ + [4]Al3+, where [6]Me2+ = Mg,Fe) or plagioclase-like ([9]Ca2+ + [4]Al3+ ↔ [9]Na+ + [4]Si4+) substitutions. Zones with a relatively high Si content (Si-rich) have pronounced indications of dissolution, while silicon-depleted zones (Si-poor) overgrow Si-rich zones, eventually creating a visible replacement zone of the crystal. We suggest that Si-poor tourmaline results from the Si-rich tourmaline losing Si during a metasomatic process. The resulting empirical crystal-chemical formula for the Si-poor zone is: X(Ca0.95Na0.03☐0.02)Σ1.00Y(Mg1.08 Al0.98Fe0.502+Fe0.433+)Σ3.00Z(Al5.91Fe0.093+)Σ6.00T[(Si4.57Al1.43)Σ6.00O18] (BO3)3V(OH)3W[O0.95(OH)0.05]Σ1.00 [a = 15.9811(2), c = 7.12520(10) Å, R1 = 1.7%] and for the Si-rich zone is: X(Ca0.89Na0.11)Σ1.00Y(Mg1.55Al0.80Fe0.342+Fe0.313+)Σ3.00Z(Al5.51Mg0.44Fe0.053+)Σ6.00T[(Si5.35Al0.65)Σ6.00O18] (BO3)3V(OH)3W[O0.93(OH)0.07]Σ1.00 [a = 15.9621(3), c = 7.14110(10) Å, R1 = 1.7%]. According to pressure-temperature (P-T) calculations of mineral assemblage stability and comparable data on synthetic [4]Al-rich tourmalines, the studied tourmaline was formed at 600–750 °C and 0.10–0.20 GPa. The formation of tetrahedral Al-rich tourmalines requires several unusual factors: (1) desilication of primary rocks and (2) high temperatures and relatively low pressures.
Characterization and potential toxicity of asbestiform erionite from Gawler Downs, New Zealand
Authors: Patel Janki Prakash , Brook Martin , Kah Melanie , Hamilton Ayrton , Gamberini Maria Cristina , Zoboli Carlotta , Mugnaioli Enrico , Malferrari Daniele , Fantini Riccardo , Arletti Rossella , Gualtieri Alessandro F. 
Published: February 2024

Abstract
Erionite is the name for a zeolite mineral series originating from diagenesis or hydrothermal alteration of volcanic rocks. The particular erionite “species” is based on the dominant extra-framework cation, erionite-Ca, erionite-K, or erionite Na. Irrespective of the species, erionite can display a fibrous/asbestiform morphology and has been linked with cases of malignant mesothelioma, a disease typically associated with asbestos exposure. Characterization of new discoveries of erionite is therefore important to assess any potential exposure hazards. This study describes a new asbestiform erionite from vesicles within the Upper Cretaceous Mt. Somers Volcanics Group (MSVG), Canterbury, New Zealand. The erionite is within the Hinds River Dacite, the youngest unit within the MSVG at Gawler Downs, ~100 km west of Christchurch, in the foothills of the Southern Alps. A multi-analytical approach was taken to analyze the sample which included micro-Raman spectroscopy, thermogravimetric analysis, electron microscopy, electron microprobe analysis, and X-ray powder diffraction with the Rietveld method. Results confirmed the mineral as fibrous erionite-K. The chemical composition of the mineral is unique due to the presence of higher levels of Mg. While Fe was also identified, this was due to smectite flakes occurring on the surface of the erionite fibers. According to the World Health Organization (WHO) respirable mineral fiber definition, where length ≥5 μm, width ≤3 μm, and aspect ratio (L/w) ≥3:1, the Gawler Downs erionite fibers are respirable, while the fibers themselves exceed respirable thickness. In addition to morphology, a value for the potential toxicity model was computed to be 2.28 for the Gawler Downs erionite. This is similar to those of other carcinogenic erionites from Karain, Turkey (2.33), and Nevada, U.S.A. (2.28). Taken together, results indicate Gawler Downs erionite represents an environmental hazard. Nevertheless, further investigation is required to determine potential environmental exposure pathways by which erionite may become airborne and assess the actual environmental risk in the Gawler Downs area.
Multi-factor coupled thermal simulation of flat-panel digital PCR structure
Authors: Li Qixuan , Qin Xulei , Wang Haoyu 
Published: February 2024

To achieve uniform reaction temperature in flat-plate digital polymerase chain reaction (dPCR), we propose a multi-factor coupled thermal simulation method for the structure of flat-plate digital PCR using finite element analysis. This will help us obtain the optimal method for the structure of flat-plate dPCR. Thermal simulations were conducted to analyze the effects of forced air cooling, thermoelectric cooler (TEC) arrangement spacing, and heat-conducting plate thickness on the temperature uniformity of the flat-plate dPCR. The resulting isothermal surfaces and velocity magnitude vectors were used to summarize the impact of each factor. The study found that maintaining a mechanical fan speed of 3000 revolutions per minute (RPM) ±10 % during the heating period resulted in a 29.3 % reduction in the standard deviation of the temperature on the surface of the heat-conducting plate. Additionally, when the TEC spacing was between 2 mm and 3.5 mm, the standard deviation of the temperature on the plate's surface decreased by 87.1 % to 93.4 %. When the thickness of the thermal plate ranges from 3.5 mm to 4.5 mm, the standard deviation of the temperature on the surface of the thermal plate varies by approximately 0.006. The experimental results, obtained by sampling and analyzing the temperature on the surface of the thermal plate, are consistent with the simulation results. This proves that the method is informative in determining the structural parameters of the dPCR to enhance temperature uniformity.
Research on automatic detection of gradual fault of high voltage electric energy metering transformer based on fuzzy rough set and whale optimization algorithm
Authors: Wang Chunguang , Wu Zhiwu , Huang Tianfu , Wu Xiang , Huang Hanbin 
Published: February 2024

Based on fuzzy rough set and whale optimization algorithm, the automatic fault detection method of high-voltage electric energy metering transformer is studied to improve the fault diagnosis effect and efficiency. On the basis of constructing the mathematical model of gradual fault of high-voltage electric energy metering transformer, the fuzzy rough set theory is used to reduce the data attributes of fault samples, eliminate similar attributes, determine the minimum fault feature set, and complete the fault feature selection, which is used as the input of the fault detection model based on Whale Optimization Algorithm-based Support Vector Machine (WOA-SVM). After the kernel parameters and penalty factors of SVM are optimized by whale optimization algorithm, the type of gradual fault of high-voltage electric energy metering transformer is identified. The experimental results show that the reduced fault attributes are distributed differently in the sample data, and the fault detection accuracy can be improved by 9.5 % through fault feature selection. The fault diagnosis model with Gaussian radial basis function, kernel parameter of 0.05 and penalty factor of 10 has the best performance. This method can identify the gradual fault types of high-voltage electric energy metering transformers, and the fault diagnosis effect is outstanding.
Seasonal quality and statistical assessment of physicochemical properties and base saturation of Hadejia-Nguru wetland soils, Northwest/Eastern Nigeria
Authors: Mohammed Sa'adatu Eri , Aderibigbe Deborah Olubunmi , Saeed Muhammad Dayyab , Nelana Simphiwe Maurice , Musa Ahmadu , Sunday Ayanda Olushola 
Published: February 2024

Abstract

The evaluation of Hadejia-Nguru wetland soils was carried out for two consecutive dry seasons. Standard analytical techniques were adopted to analyze the samples and statistical models were used to assess the spatial and temporal distribution of the parameters selected. The average pH values of the soil samples were mostly alkaline, and all the measured values were also within the approved standards. Experimental results revealed a significantly positive correlation between Ca and Mg for both seasons as indicated by the p-values and a high coefficient of determination (R2) for both metals with cation exchange capacity (CEC) values as the predictors. The regression analysis showed that the changes in CEC and pH levels in the soil were dependent on changes in Ca and Mg content of the soil. The data obtained showed that the increase in pH is directly proportional to the CEC and base saturation of the soils. A higher percentage of Ca than other cations (Mg, K and Na) was observed from the base saturation of the soils. The pH, base saturation, and CEC levels in the soils are suitable for most crops grown in the area, which has the potential to support a wide range of crops with further exploration.