bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gene expression of CALD1, CDH2, and POSTN in fibroblast are related to idiopathic pulmonary fibrosis
Authors: Wu Shufei , Liu Mengying , Zhang Mingrui , Ye Xu , Gu Huimin , Jiang Cheng , Zhu Huihui , Ye Xiaoling , Li Qi , Huang Xinmei , Cao Mengshu 
Published: February 2024

IntroductionIdiopathic pulmonary fibrosis (IPF) is characterized by progressive lung dysfunction due to excessive collagen production and tissue scarring. Despite recent advancements, the molecular mechanisms remain unclear.MethodsRNA sequencing identified 475 differentially expressed genes (DEGs) in the TGF-β1-induced primary lung fibrosis model. Gene expression chips GSE101286 and GSE110147 from NCBI gene expression omnibus (GEO) database were analyzed using GEO2R, revealing 94 DEGs in IPF lung tissue samples. The gene ontology (GO) and pathway enrichment, Protein-protein interaction (PPI) network construction, and Maximal Clique Centrality (MCC) scoring were performed. Experimental validation included RT-qPCR, Immunohistochemistry (IHC), and Western Blot, with siRNA used for gene knockdown. A co-expression network was constructed by GeneMANIA.ResultsGO enrichment highlighted significant enrichment of DEGs in TGF-β cellular response, connective tissue development, extracellular matrix components, and signaling pathways such as the AGE-RAGE signaling pathway and ECM-receptor interaction. PPI network analysis identified hub genes, including FN1, COL1A1, POSTN, KIF11, and ECT2. CALD1 (Caldesmon 1), CDH2 (Cadherin 2), and POSTN (Periostin) were identified as dysregulated hub genes in both the RNA sequencing and GEO datasets. Validation experiments confirmed the upregulation of CALD1, CDH2, and POSTN in TGF-β1-treated fibroblasts and IPF lung tissue samples. IHC experiments probed tissue-level expression patterns of these three molecules. Knockdown of CALD1, CDH2, and POSTN attenuated the expression of fibrotic markers (collagen I and α-SMA) in response to TGF-β1 stimulation in primary fibroblasts. Co-expression analysis revealed interactions between hub genes and predicted genes involved in actin cytoskeleton regulation and cell-cell junction organization.ConclusionsCALD1, CDH2, and POSTN, identified as potential contributors to pulmonary fibrosis, present promising therapeutic targets for IPF patients.
Quantitative multiplex immunohistochemistry reveals inter-patient lymphovascular and immune heterogeneity in primary cutaneous melanoma
Authors: Femel Julia , Hill Cameron , Illa Bochaca Irineu , Booth Jamie L. , Asnaashari Tina G. , Steele Maria M. , Moshiri Ata S. , Do Hyungrok , Zhong Judy , Osman Iman , Leachman Sancy A. , Tsujikawa Takahiro , White Kevin P. , Chang Young H. , Lund Amanda W. 
Published: February 2024

IntroductionQuantitative, multiplexed imaging is revealing complex spatial relationships between phenotypically diverse tumor infiltrating leukocyte populations and their prognostic implications. The underlying mechanisms and tissue structures that determine leukocyte distribution within and around tumor nests, however, remain poorly understood. While presumed players in metastatic dissemination, new preclinical data demonstrates that blood and lymphatic vessels (lymphovasculature) also dictate leukocyte trafficking within tumor microenvironments and thereby impact anti-tumor immunity. Here we interrogate these relationships in primary human cutaneous melanoma. MethodsWe established a quantitative, multiplexed imaging platform to simultaneously detect immune infiltrates and tumor-associated vessels in formalin-fixed paraffin embedded patient samples. We performed a discovery, retrospective analysis of 28 treatment-naïve, primary cutaneous melanomas. ResultsHere we find that the lymphvasculature and immune infiltrate is heterogenous across patients in treatment naïve, primary melanoma. We categorized five lymphovascular subtypes that differ by functionality and morphology and mapped their localization in and around primary tumors. Interestingly, the localization of specific vessel subtypes, but not overall vessel density, significantly associated with the presence of lymphoid aggregates, regional progression, and intratumoral T cell infiltrates. DiscussionWe describe a quantitative platform to enable simultaneous lymphovascular and immune infiltrate analysis and map their spatial relationships in primary melanoma. Our data indicate that tumor-associated vessels exist in different states and that their localization may determine potential for metastasis or immune infiltration. This platform will support future efforts to map tumor-associated lymphovascular evolution across stage, assess its prognostic value, and stratify patients for adjuvant therapy.
Predicting progression of cognitive decline to dementia using dyadic patterns of subjective reporting: evidence from the CompAS longitudinal study
Authors: Pérez-Blanco Lucía , Felpete-López Alba , Nieto-Vieites Ana , Lojo-Seoane Cristina , Campos-Magdaleno María , Fernández-Feijoo Fátima , Juncos-Rabadán Onésimo , Pereiro Arturo X. 
Published: February 2024

ObjectiveTo analyze the validity of self and informant reports, depressive symptomatology, and some sociodemographic variables to predict the risk of cognitive decline at different follow-up times.MethodsA total of 337 participants over 50 years of age included in the CompAS and classified as Cognitively Unimpaired (CU), Subjective Cognitive Decline (SCD) and Mild Cognitive Impairment (MCI) groups were assessed at baseline and three follow-ups. A short version of the QAM was administered to assess the severity of subjective cognitive complaints (SCCs), and the GDS-15 was used to evaluate the depressive symptoms. At each follow-up assessment, participants were reclassified according to the stability, regression or progression of their conditions. Logistic regression analysis was used to predict which CU, SCD and MCI participants would remain stable, regress or progress at a 3rd follow-up by using self- and informant-reported complaints, depressive symptomatology, age and education at baseline and 2nd follow-ups as the predictive variables.ResultsOverall, self-reported complaints predicted progression between the asymptomatic and presymptomatic stages. As the objective deterioration increased, i.e., when SCD progressed to MCI or dementia, the SCCs reported by informants proved the best predictors of progression. Depressive symptomatology was also a predictor of progression from CU to SCD and from SCD to MCI.ConclusionA late increase in self-reported complaints make valid estimates to predict subjective decline at asymptomatic stages. However, an early increase in complaints reported by informants was more accurate in predicting objective decline from asymptomatic stages. Both, early and late decrease in self-reported complaints successfully predict dementia from prodromic stage. Only late decrease in self-reported complaints predict reversion from prodromic and pre-symptomatic stages.
Dielectric assist accelerating structures for compact linear accelerators of low energy particles in hadrontherapy treatments
Authors: Martinez-Reviriego Pablo , Esperante Daniel , Grudiev Alexej , Gimeno Benito , Blanch César , González-Iglesias Daniel , Fuster-Martínez Nuria , Martín-Luna Pablo , Martínez Eduardo , Menendez Abraham , Fuster Juan 
Published: February 2024

Dielectric Assist Accelerating (DAA) structures based on ultralow-loss ceramic are being studied as an alternative to conventional disk-loaded copper cavities. This accelerating structure consists of dielectric disks with irises arranged periodically in metallic structures working under the TM02-π mode. In this paper, the numerical design of an S-band DAA structure for low beta particles, such as protons or carbon ions used for Hadrontherapy treatments, is shown. Four dielectric materials with different permittivity and loss tangent are studied as well as different particle velocities. Through optimization, a design that concentrates most of the RF power in the vacuum space near the beam axis is obtained, leading to a significant reduction of power loss on the metallic walls. This allows to fabricate cavities with an extremely high quality factor, over 100,000, and shunt impedance over 300 MΩ/m at room temperature. During the numerical study, the design optimization has been improved by adjusting some of the cell parameters in order to both increase the shunt impedance and reduce the peak electric field in certain locations of the cavity, which can lead to instabilities in its normal functioning.
Impact of prolonged exposure to occasional and regular waterpipe smoke on cardiac injury, oxidative stress and mitochondrial dysfunction in male mice
Authors: Hamadi Naserddine , Al-Salam Suhail , Beegam Sumaya , Zaaba Nur Elena , Elzaki Ozaz , Nemmar Abderrahim 
Published: February 2024

Regular waterpipe smoking (Reg-WPS) is well recognized for its deleterious effect on the heart. However, there is a paucity of experimental studies on the impact of occasional waterpipe smoking (Occ-WPS), also known as nondaily smoking, versus Reg-WPS on cardiac homeostasis, and the mechanisms underlying these effects. Hence, we aimed, in the present study, to investigate the effect of Occ-WPS (30 min/day, 1 day/week) versus Reg-WPS (30 min/day, 5 days/week) for 6 months on systolic blood pressure (SBP), cardiac injury, oxidative markers, chemokines, proinflammatory cytokines, DNA damage and mitochondrial function compared with air (control) exposed mice. Our results show that SBP was increased following exposure to either Occ-WPS or Reg-WPS compared with air-exposed mice. Moreover, we found that only Reg-WPS induced a significant elevation in the levels of troponin I, brain natriuretic peptide, lactate dehydrogenase, and creatine phosphokinase. However, the atrial natriuretic peptide (ANP) was significantly increased in both Occ-WPS and Reg-WPS groups. Compared with air-exposed mice, the levels of lipid peroxidation, reduced glutathione and monocyte chemoattractant protein-1 were only significantly augmented in the Reg-WPS. However, catalase, superoxide dismutase, and CXCL1 were significantly increased in both Occ-WPS and Reg-WPS. The concentrations of the adhesion molecules E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 were solely elevated in the heart of mice exposed to Reg-WPS. Similarly, the concentrations of interleukin-1β and tumor necrosis factor α were only significantly augmented in the Reg-WPS. However, both Occ-WPS and Reg-WPS triggered significant augmentation in the levels of IL17 and DNA damage compared to the control groups. Furthermore, while Occ-WPS induced a slight but statistically insignificant elevation in the concentrations of mammalian targets of rapamycin and nuclear factor erythroid-derived 2-like 2 (Nrf2) expression, Reg-WPS exposure increased their levels substantially, in addition to p53 and mitochondrial complexes II &amp; III, and IV activities compared with air-exposed mice. In conclusion, our findings show that while the long-term Occ-WPS exposure induced an elevation of SBP, ANP, antioxidant enzymes, IL17, CXCL1, and cardiac DNA damage, Reg-WPS exposure was consistently associated with the elevation of SBP and occurrence of cardiac damage, inflammation, oxidative stress, DNA damage and mitochondrial dysfunction.
Precision MRI phenotyping of muscle volume and quality at a population scale
Authors: Thanaj Marjola , Basty Nicolas , Whitcher Brandon , Sorokin Elena P. , Liu Yi , Srinivasan Ramprakash , Cule Madeleine , Thomas E. Louise , Bell Jimmy D. 
Published: February 2024

Introduction: Magnetic resonance imaging (MRI) enables direct measurements of muscle volume and quality, allowing for an in-depth understanding of their associations with anthropometric traits, and health conditions. However, it is unclear which muscle volume measurements: total muscle volume, regional measurements, measurements of muscle quality: intermuscular adipose tissue (IMAT) or proton density fat fraction (PDFF), are most informative and associate with relevant health conditions such as dynapenia and frailty.Methods: We have measured image-derived phenotypes (IDPs) including total and regional muscle volumes and measures of muscle quality, derived from the neck-to-knee Dixon images in 44,520 UK Biobank participants. We further segmented paraspinal muscle from 2D quantitative MRI to quantify muscle PDFF and iron concentration. We defined dynapenia based on grip strength below sex-specific cut-off points and frailty based on five criteria (weight loss, exhaustion, grip strength, low physical activity and slow walking pace). We used logistic regression to investigate the association between muscle volume and quality measurements and dynapenia and frailty.Results: Muscle volumes were significantly higher in male compared with female participants, even after correcting for height while, IMAT (corrected for muscle volume) and paraspinal muscle PDFF were significantly higher in female compared with male participants. From the overall cohort, 7.6% (N = 3,261) were identified with dynapenia, and 1.1% (N = 455) with frailty. Dynapenia and frailty were positively associated with age and negatively associated with physical activity levels. Additionally, reduced muscle volume and quality measurements were associated with both dynapenia and frailty. In dynapenia, muscle volume IDPs were most informative, particularly total muscle exhibiting odds ratios (OR) of 0.392, while for frailty, muscle quality was found to be most informative, in particular thigh IMAT volume indexed to height squared (OR = 1.396), both with p-values below the Bonferroni-corrected threshold (p<8.8×10−5).Conclusion: Our fully automated method enables the quantification of muscle volumes and quality suitable for large population-based studies. For dynapenia, muscle volumes particularly those including greater body coverage such as total muscle are the most informative, whilst, for frailty, markers of muscle quality were the most informative IDPs. These results suggest that different measurements may have varying diagnostic values for different health conditions.
Predictors of body image dissatisfaction in kinesiology students
Authors: Jakobek Vedran , Kranjčev Mislav , Barić Renata 
Published: February 2024

IntroductionThis research aimed to examine predictors of discrepancies between actual and ideal body images, specifically body fat and muscularity discrepancies, in kinesiology students.MethodsVolunteer participants (N = 174, men = 112, women = 62) filled out the sex-specific Somatomorphic Matrices (NSM-M and SM-F) as well as The Positive and Negative Perfectionism Scale (PNPS), Rosenberg Self-esteem Scale (RSE), and the Eating Attitudes Test (EAT-26) using paper and pencil tools.ResultsThe median and Mann–Whitney U tests were used to determine sex differences, and regression analyses were used to determine the contribution of sex, perfectionism, self-esteem, and eating attitudes in explaining the variances in body fat and muscularity dissatisfaction. The results showed no sex differences in body fat discrepancy, while men showed more discrepancy between actual and ideal muscularity than women. In regression analyses, sex was the only significant predictor of muscularity discrepancy, while disordered eating attitudes were a positive, and positive perfectionism was a negative predictor of body fat discrepancy.DiscussionThis study showed that while muscularity dissatisfaction is linked to the male sex, body fat dissatisfaction is not related to sex but to psychological variables of disordered eating attitudes and positive perfectionism in a population of youths that may be above average when it comes to physical activity.
One independent or many independent? The relationship among self-construal, number of brand endorsers, and brand attitudes
Authors: Liang Shichang , Cai Kunhan , Zhang Yiwei , Yuan Xueying , Pan Siyu , Teng Lili 
Published: February 2024

IntroductionIt was common for brands to use different numbers of endorsers in marketing practice. Nevertheless, research on brand endorsers’ quantity has not yielded a uniform consensus. The previous research about brand endorsers mainly focuses on the appeal of endorsement, brand category, and endorser characteristics, paying less attention to the impact of cultural factors, particularly self-construal. This study delves into selecting brand endorsers across diverse cultural regions for the same brand.MethodsDrawing on the principles of self-consistency theory and self-construal theory, our research, conducted through three distinct experiments, reveals that consumers tend to hold more favorable opinions about brands endorsed by a single individual. Furthermore, self-consistency emerges as a crucial mediating factor in this phenomenon. Additionally, self-construal is an essential factor among consumers from various cultural backgrounds.ResultsConsumers with an independent self-construal exhibit more favorable brand perceptions when it comes to single-endorser brands compared to their counterparts with an interdependent self-construal. Conversely, individuals with an interdependent self-construal demonstrate a more positive disposition towards brands with multiple endorsers than those with an independent self-construal.DiscussionThis research not only enriches and extends our theoretical understanding of the impact of the number of brand endorsers on consumer brand attitudes but also provides valuable practical insights for optimizing the selection of brand endorsers for companies.
Implementation of a Budo group therapy for psychiatric in- and outpatients: a feasibility study
Authors: Singh Jasprit , Jawhari Karl , Jaffé Mariela , Imfeld Lukas , Rabenschlag Franziska , Moeller Julian , Nienaber André , Lang Undine E. , Huber Christian G. 
Published: February 2024

IntroductionPhysical exercise has been shown to have numerous health benefits on co-morbid somatic conditions in psychiatry and can also enhance mental health. Thus, it is not difficult to recommend physical training programs as part of an integrated and holistic treatment approach for mental health disorders. However, getting patients to participate and keeping them engaged is a major challenge. Programs based on martial arts training could be interventions improving physical and mental health with higher attachment rates. The structured discipline, holistic approach integrating physical and mental elements, and empowering activities, may explain higher participant attachment rates.MethodsThus, the main objective of this feasibility study is to describe a newly established group therapy program incorporating interventions from martial arts training with its physical and philosophical parts including mindfulness and breath work.ResultsDuring the 14-month study period from April 2021 to May 2022, a Budo group therapy was used by 215 individual persons with a total of 725 group therapy participations. Retention in the program was good across all settings and very good for persons who participated as outpatients. The mean age of the participants was 33.5 years with a range from 14 to 69 years of age, and about 41% of the participants were female. The therapy program was able to address patients over the whole spectrum of psychiatric diagnoses. Satisfaction and motivation were uniformly self-reported as very good. Patients self-reported improved mental and physical health after participating in a Budo session compared to pre-session.DiscussionBudo group therapy thus can be seen as a feasible, well-accepted and promising new transdiagnostic treatment approach, combining physical activation with resilience enhancement. With minimal contraindications, a broad spectrum of individuals seeking mental health support can engage in this group therapy.
Implementation and analysis of a parallel kalman filter algorithm for lidar localization based on CUDA technology
Authors: Mochurad Lesia 
Published: February 2024

Introduction: Navigation satellite systems can fail to work or work incorrectly in a number of conditions: signal shadowing, electromagnetic interference, atmospheric conditions, and technical problems. All of these factors can significantly affect the localization accuracy of autonomous driving systems. This emphasizes the need for other localization technologies, such as Lidar.Methods: The use of the Kalman filter in combination with Lidar can be very effective in various applications due to the synergy of their capabilities. The Kalman filter can improve the accuracy of lidar measurements by taking into account the noise and inaccuracies present in the measurements.Results: In this paper, we propose a parallel Kalman algorithm in three-dimensional space to speed up the computational speed of Lidar localization. At the same time, the initial localization accuracy of the latter is preserved. A distinctive feature of the proposed approach is that the Kalman localization algorithm itself is parallelized, rather than the process of building a map for navigation. The proposed algorithm allows us to obtain the result 3.8 times faster without compromising the localization accuracy, which was 3% for both cases, making it effective for real-time decision-making.Discussion: The reliability of this result is confirmed by a preliminary theoretical estimate of the acceleration rate based on Ambdahl’s law. Accelerating the Kalman filter with CUDA for Lidar localization can be of significant practical value, especially in real-time and in conditions where large amounts of data from Lidar sensors need to be processed.
Expression of PD-L1 and p-RPS6 in epithelial dysplasia and squamous cell carcinoma of the oral cavity
Authors: Hanroongsri Jaruwat , Amornphimoltham Panomwat , Younis Rania H. , Chaisuparat Risa 
Published: February 2024

IntroductionOral squamous cell carcinoma (OSCC) is often preceded by oral epithelial dysplasia (OED). The role of ribosomal protein S6 (RPS6) and programmed cell death ligand-1 (PD-L1) in the progression of OED to OSCC remains unclear. This study aimed to investigate the expression of phosphorylated RPS6 (p-RPS6) and PD-L1 in OSCC and OED and to examine its relationship with clinicopathological features.MethodsFifty-two OSCC and 48 OED cases were recruited for immunohistochemical analysis of p-RPS6 and PD-L1 expression. The expression of markers was correlated with clinicopathological features of OSCC and OED.ResultsWe found p-RPS6 expression in all cases of OSCC and OED, whereas PD-L1 was expressed in 42/48 (87%) OED and in 28/52 (53%) OSCC. The patients with mild OED presented higher expression level of PD-L1 and p-RPS6 significantly, when compared to moderate-differentiated OSCC patients (p &lt; 0.05). Moreover, we found a significant positive correlation between PD-L1 and p-RPS6 expression in OED and OSCC patients (p &lt; 0.01). The PD-L1 expression was significantly related to more than 2 cm tumor size in OSCC patients (p = 0.007).DiscussionOur findings suggest the upregulation of PD-L1 may be related with activation of the mTOR pathway in the early events of tumor progression and the pathogenesis of OSCC.