bluuebunny/crossref_metadata_2025_split huggingface dataset:
RANCANG BANGUN APLIKASI E-BUDGETING UNTUK MEMONITOR REALISASI ANGGARAN SPN POLDA SULSEL BERBASIS WEB
Authors: Hasan Fadlan , Belluano Poetri Lestari Lokapitasari , Manga Abdul Rachman 
Published: January 2025

Sekolah Polisi Negara (SPN) merupakan bagian dari unit organisasi Polri dibawah naungan kepolisian daerah yang melaksanakan tugas pokok sebagai unsur pelaksana lembaga pendidikan Polri mengemban tugas melaksanakan program pendidikan dan pelatihan tingkat kepolisian daerah. Proses penganggaran pada SPN Polda Sulawesi Selatan mulai dari tahapan awal pembuatan DIPA, proses pengajuan rencana kebutuhan anggaran, realisasi anggaran hingga tahap terakhir pelaporan realisasi anggaran masih belum memiliki sistem yang mampu mengolah dan mengendalikan data dengan baik. Aplikasi E-Budgeting berbasis website yang dapat diakses admin, Renmin, Para Staf dan Kepala SPN sebagai media untuk mempermudah Pihak SPN Polda Sulsel dalam mengelola data anggaran sampai pembuatan laporan realisasi anggaran yang sekiranya diakses secara online terhubung dengan internet. Dalam merancang aplikasi E-Budgeting peneliti menggunakan pemodelan perangkat lunak Unified Modelling Language (UML) yang berorientasi pada objek. Proses pemodelan UML ini dimulai dengan menganalisis kebutuhan sistem dan diimplementasikan pada usecase diagram lalu dilanjutkan dengan membuat class diagram, sequence diagram dan activity diagram. Metode penelitian yang digunakan adalah Model System Development Life Cycle (SDLC) Waterfall merupakan sebuah model pengembangan perangkat lunak yang menyediakan pendekatan alur hidup perangkat lunak secara sekuensial atau terurut dimulai dari analisis kebutuhan, perancangan, pengujian, impelentasi dan pemeliharaan. Hasil pengujian menunjukkan bahwa aplikasi e-budgeting yang dibangun sudah memenuhi standar yang telah ditentukan dan mampu mengakomodir seluruh proses penganggaran sehingga dapat meningkatkan efektifitas, efisiensi, dan relevan dalam proses penganggaran SPN Polda Sulsel.
Revealing the biological features of the axolotl pancreas as a new research model
Authors: Ma Hui , Peng Guangcong , Hu Yan , Lu Binbin , Zheng Yiying , Wu Yingxian , Feng Weimin , Shi Yu , Pan Xiangyu , Song Li , Stützer Ina , Liu Yanmei , Fei Jifeng 
Published: January 2025

IntroductionThe pancreas plays a crucial role in digestion and blood glucose regulation. Current animal models, primarily mice and zebrafish, have limited the exploration of pancreatic biology from an evolutionary-developmental perspective. Tetrapod vertebrate axolotl (Ambystoma mexicanum) serves as a valuable model in developmental, regenerative, and evolutionary biology. However, the fundamental biology of the axolotl pancreas remains underexplored. This study aims to characterize the unique developmental, functional, and evolutionary features of the axolotl pancreas to expand the understanding of pancreatic biology.MethodsWe conducted morphological, histological, and transcriptomic analyses to investigate the axolotl pancreas. Pancreatic development was observed using in situ hybridization and immunostaining for key pancreatic markers. RNA sequencing was performed to profile global gene expression during larva and adult stages. And differential gene expression analysis was used to characterize the conserved and unique gene patterns in the axolotl pancreas. Functional assays, including glucose tolerance tests and insulin tolerance tests, were optimized for individual axolotls. To assess pancreatic gene function, Pdx1 mutants were generated using CRISPR/Cas9-mediated gene editing, and their effects on pancreatic morphology, endocrine cell populations, and glucose homeostasis were analyzed.ResultsThe axolotl pancreas contains all known pancreatic cell types and develops from dorsal and ventral buds. Both of buds contribute to exocrine and endocrine glands. The dorsal bud produces the major endocrine cell types, while the ventral bud generates α and δ cells, but not β cells. Differential gene expression analysis indicated a transition in global gene expression from pancreatic cell fate commitment and the cell cycle to glucose response, hormone synthesis, and secretion, following the development progression. Notably, the adult axolotl pancreas exhibits slower metabolic activity compared to mammals, as evidenced by the results of GTT and ITT. The mutation of Pdx1 resulted in hyperglycemia and a significant reduction in pancreatic cell mass, including a complete loss of endocrine cells, although it did not lead to a lethal phenotype.DiscussionThis study examines the axolotl pancreas, highlighting the conservation of pancreatic development. Our study highlights the unique features of the axolotl pancreas and broadens the scope of animal models available for pancreatic evolution and disease research.
A novel strategy for eradication of staphylococcal biofilms using blood clots
Authors: Grooters Kayla E. , Hayes Sheridan L. , Richter David M. , Ku Jennifer C. , Sawyer Robert , Li Yong 
Published: January 2025

IntroductionInfections with coagulase negative staphylococcal species (CoNS) are a major cause of mortality and morbidity in joint and heart valve replacement procedures, largely due to biofilm formation. Cells within biofilms have higher rates of antibiotic resistance than their planktonic counterparts; consequently, novel mechanisms are needed to combat these infections.MethodsTo enhance antibiotic delivery and penetration, this innovative study involved treating CoNS biofilms with murine blood clots impregnated with antibiotics. We then investigated the impact of this treatment on biofilm density, metabolism, and architecture.ResultsOur pilot study demonstrates that this method of antibiotic delivery results in improved biofilm clearance, relative to conventional exposure methods.DiscussionOur results demonstrate that blood clot exposure has an intrinsic impact on biofilm density and potentially reduces colonization, warrenting further investigation into the mechanism.
Amorphous silica reduces N2O emissions from arable land at the field plot scale
Authors: Hoffmann Mathias , Diaz Oscar Rodrigo Monzon , Zentgraf Isabel , Al-Hamwi Wael , Dubbert Maren , Stein Mathias , Holz Maire , Schaller Jörg 
Published: January 2025

IntroductionIncreasing greenhouse gas emissions pose a strong threat due to accelerating global warming. N2O emissions are highly important in this regard as N2O is a very powerful greenhouse gas. Agriculture is the main human-induced source for N2O emissions, contributing roughly 60% to total N2O emissions. Soil amorphous silica (ASi) contents are reduced in arable soils due to yearly exports by crop harvest as most crops are silicon accumulator plants. Most recently it has been shown that ASi is increasing water and nutrient availability in soils. Both factors are known to directly and indirectly affect N2O emissions from agroecosystems.MethodsIn this study we conducted a field plot trial on arable soil depleted in ASi and fertilized this soil to its pre-agricultural ASi level.ResultsOur data clearly shows that increasing soil ASi to a pre-agricultural level decreased seasonal N2O emissions by ∼30%.DiscussionThis reduction of N2O emissions due to ASi might be of global relevance as agricultural practice has reduced the ASi content in agricultural soils. If future studies confirm the effect of ASi on N2O emissions, the soil ASi depletion by agricultural practice in the last decades may have led to a substantial increase of N2O emissions.
Anemia during pregnancy and adverse pregnancy outcomes: a systematic review and meta-analysis of cohort studies
Authors: Wang Rui , Xu Shan , Hao Xiaoyu , Jin Xingyi , Pan Da , Xia Hui , Liao Wang , Yang Ligang , Wang Shaokang 
Published: January 2025

ObjectivesAnemia in pregnancy has been a topic of interest for researchers due to its potential impact on various adverse pregnancy outcomes. This study aims to explore the relationship between anemia and adverse pregnancy outcomes such as preterm birth, low birth weight, and maternal mortality.MethodsWe conducted both a systematic review and a meta-analysis on the associations between anemia during pregnancy and adverse pregnancy outcomes. We searched Chinese databases (CNKI, Wanfang, CBM, VIP) and English ones (Cochrane Library, PubMed, Embase, Web of Science). Two researcher-authors independently assessed study quality with the Newcastle-Ottawa Scale. After extracting data, we analyzed heterogeneity and used a random-effects model for higher heterogeneity and a fixed-effects model for low heterogeneity in the meta-analysis while also systematically synthesizing and narratively describing findings in the systematic review.ResultsA total of 31 cohort studies were included. Meta-analysis showed that the risk of postpartum hemorrhage [RR [95% CI], 2.76 [1.63, 4.66]], premature rupture of membranes (PROM) [1.94 (1.26, 3.00)], preterm delivery [1.51 (1.33, 1.72)], low birth weight (LBW) [1.40 (1.19, 1.63)], cesarean section[1.33 (1.02, 1.74)], gestational hypertension[1.28 (1.14, 1.44)] and neonatal asphyxia[1.21 (1.07, 1.37)] was higher in the group of anemia in pregnancy than in the control group.ConclusionMaternal anemia is associated with an increased risk of seven adverse pregnancy outcomes: postpartum hemorrhage, PROM, preterm delivery, LBW, cesarean section, gestational hypertension and neonatal asphyxia. Appropriate nutritional supplementation and screening for anemia before and during pregnancy are recommended to improve maternal health and manage adverse pregnancy outcomes.
Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation
Authors: Chen Shuxiao , Liu Xiaotian , Zhou Xincheng , Lin Weixiao , Liu Minting , Ma Haoran , Zhong Keli , Ma Qiming , Qin Chengjian 
Published: January 2025

BackgroundAbdominal aortic aneurysm (AAA) is a degenerative disease with high mortality. Chronic inflammation plays a vital role in the formation of AAA. Atractylenolide-I (ATL-I) is a major bioactive component of Rhizoma Atractylodis Macrocephalae that exerts anti-inflammatory effects in various diseases. The purpose of this study is to investigate the role of ATL-I in the progression of AAA.MethodsAAA was constructed in C57BL/6 mice by porcine pancreatic elastase (PPE)-incubation, and the diameter of the aorta was measured by ultrasound. ATL-I was administered by gavage on the second day after modeling to explore its significance in AAA. The pathological and molecular alteration was investigated by immunostaining, ELISA, qRT-PCR and Western blotting.ResultsATL-I inhibited the dilatation of the abdominal aorta and decreased the incidence of AAA. ATL-I alleviated the infiltration of macrophages in the adventitia and reduced the levels of proinflammatory factor IL-1β and IL-6 in the aorta and circulatory system, while increasing the expression of anti-inflammatory factor IL-10. Moreover, ATL-I restrained loss of smooth muscle cells and elastic fiber degradation by suppressing MMP-2 and MMP-9 expression. Mechanistically, phospho-AMPK expression was elevated in AAA groups, and ATL-I administration suppressed its expression to improve the pathological damage of aorta.ConclusionsATL-I meliorated vascular inflammation by targeting AMPK signaling, ultimately inhibiting AAA formation, which provided an alternative agent for AAA treatment.
Outcomes with bridging radiation therapy prior to chimeric antigen receptor T-cell therapy in patients with aggressive large B-cell lymphomas
Authors: Manzar Gohar S. , Pinnix Chelsea C. , Dudzinski Stephanie O. , Marqueen Kathryn E. , Cha Elaine E. , Nasr Lewis F. , Yoder Alison K. , Rooney Michael K. , Strati Paolo , Ahmed Sairah , Nze Chijioke , Nair Ranjit , Fayad Luis E. , Wang Michael , Nastoupil Loretta J. , Westin Jason R. , Flowers Christopher R. , Neelapu Sattva S. , Gunther Jillian R. , Dabaja Bouthaina S. , Wu Susan Y. , Fang Penny Q. 
Published: January 2025

BackgroundSelect patients with relapsed/refractory aggressive B cell lymphoma may benefit from bridging radiation (bRT) prior to anti-CD19-directed chimeric antigen receptor T cell therapy (CAR-T). Here, we examined patient and treatment factors associated with outcomes and patterns of failure after bRT and CAR-T.MethodsWe retrospectively reviewed adults with diffuse large B-cell lymphoma (DLBCL) who received bRT prior to axicabtagene ciloleucel, tisagenlecleucel, or lisocabtagene maraleucel between 11/2017-4/2023. Clinical/treatment characteristics, response, and toxicity were extracted. Survival was modeled using Kaplan-Meier or Cox regression models for events distributed over time, or binary logistic regression for disease response. Fisher’s Exact Test or Mann-Whitney U methods were used.ResultsOf 51 patients, 25.5% had bulky disease and 64.7% had Stage III/IV disease at the time of RT. Comprehensive bRT alone to all disease sites was delivered to 51% of patients, and 29.4% were additionally bridged with systemic therapy. Median follow-up was 10.3 months (95% CI: 7.7-16.4). Overall response rate (ORR) was 82.4% at 30 days post-CAR-T infusion. Median overall survival (OS) was 22.1 months (6.6-not reached) and the median progression-free survival (PFS) was 7.4 months (5.5-30). OS/PFS were 80% (66-99)/78% (64-87) at 1-year, and 59% (44-71)/54% (40-67) at 2-years, respectively. Comprehensive RT to all sites of disease correlated with improved PFS and OS, p ≤ 0.04. Additionally, ECOG ≥2 and Stage III/IV disease predicted poor OS (p ≤ 0.02). Disease bulk, IPI ≥3, and non-GCB histology were poor predictors for disease-specific survival (DSS), p&lt;0.05. The latter two, as well as bRT dose of ≤30 Gy predicted worse PFS (p&lt;0.05). Among patients with advanced stage disease, comprehensive bRT to all sites of disease (n=10) was not associated with improved OS and PFS compared to focal bRT (n=23), p&gt;0.17. No difference was seen in bridging RT vs. chemoRT. Twenty-six patients developed relapse (50.9%), of which 46% was in-field. Risk of in-field relapse correlated with bulky disease (OR=7, 95% CI: 1.2-41, p=0.03) and lack of response at 30 day post-CAR-T evaluation (OR=16.8, 95% CI: 1.6-176, p=0.02), but not with bRT dose (p=0.27).ConclusionbRT and CART is a good treatment strategy for select patients with aggressive B cell lymphoma. Comprehensive bRT including all sites of disease is associated with improved outcomes.
The systemic oxidative stress index predicts clinical outcomes of esophageal squamous cell carcinoma receiving neoadjuvant immunochemotherapy
Authors: Feng Jifeng , Wang Liang , Yang Xun , Chen Qixun 
Published: January 2025

BackgroundStrong correlations have been shown between systemic oxidative stress (SOS) and the occurrence, metastasis, and prognosis of many types of cancers. It is yet unknown how SOS levels relate to the prognosis of esophageal squamous cell carcinoma (ESCC). The current research aims to explore the prognostic role of systemic oxidative stress index (SOSI) on ESCC receiving neoadjuvant immunochemotherapy (nICT).MethodsRetrospective recruitment was used to identify 224 nICT-treated ESCC patients. In order to determine the integrative score of SOSI, logistic regression analyses were utilized to screen independent risk variables, with disease-free survival (DFS) serving as the dependent variable. Given the non-linear relationship between SOSI and DFS, the best threshold was determined using a restricted cubic spline (RCS) model. Independent variable determination was executed using a cox regression analysis. For prognostic prediction, a risk categorization method based on recursive partitioning analysis (RPA) was also created.ResultsFour SOS-related indicators, including albumin, creatinine, blood urea nitrogen, and direct bilirubin, were used to establish the SOSI. The ideal threshold of SOSI, shown by the non-linear relationship between DFS and SOSI (P&lt;0.001), was used to compare between two groups. As a potential prognostic factor for those nICT-treated ESCC patients, SOSI showed a strong correlation with both DFS and overall survival (OS). Patients with low SOSI had better DFS (55.1% vs. 85.5%, P&lt;0.001) and OS (72.6% vs. 79.1%, P=0.013). Then, a new staging that included TNM and SOSI based on RPA algorithms was produced. In terms of prognostication, the RPA model performed significantly better than TNM classification.ConclusionSOSI is a simple and useful score based on available SOS-related indices. In ESCC receiving nICT, low SOSI is found to be an important factor of better prognosis.
The extensive cultivation of Pyropia haitanensis along the coastal areas influences the ecological dynamics of nearby intertidal mudflat pond water
Authors: Liu Peihao , Luo Qijun , Zhu Shanshan , Chen Juanjuan , Yang Rui , Niu Tingting , Wang Tiegan , Zhang Peng , Chen Haimin 
Published: January 2025

IntroductionThe aquaculture in intertidal mudflat ponds on the coast of the East China Sea typically coexists with oceanic seaweed cultivation in the nearby open water area, forming an interconnected aquatic ecosystem. This study aimed to understand the impact of the seaweed cultivation on the water quality and microbial communities in their neighboring intertidal mudflat ponds.MethodsA unique recirculating culture system was established, integrating seaweed farming in marine areas with aquaculture ponds on mudflats, and incorporating an effluent treatment process for added efficiency. Water samples were collected from three sites, including open water areas with or without seaweed (Pyropia haitanensis) cultivation, the aquaculture mudflat ponds, and effluent water ponds, with control sites for comparison. The dynamic diversity patterns and structure of microbial communities were determined by 16S and 18S rRNA sequencing, and the environmental factors were measured.ResultsIn the offshore aquaculture area, the cultivation of P. haitanensis increased the dissolved oxygen and pH, decreased inorganic nutrients, reduced the alpha diversity of bacteria, established the dominance of Proteobacteria, but did not influence the composition of microeukaryotes. These changes in the seawater subsequently affected the dynamics in the corresponding aquaculture ponds, where the water quality was improved, the microbial diversity was enhanced, and the abundance of functional bacteria such as Microbacteriaceae was increased. According to the co-occurrence network analysis, the cultivation of P. haitanensis potentially improved the stability of the microbial communities in both the offshore aquaculture area and the onshore aquaculture ponds and allowed them to resist stress better. In addition, after the effluent from the aquaculture ponds was purified in the treatment ponds with mangroves and oysters, the water quality improved and the abundances of Dinophyceae and Bacillariophyta decreased.DiscussionThis study highlights the positive effects of P. haitanensis cultivation on water quality and microbial stability in adjacent aquaculture ponds.
Risk factors for multidrug resistance in pulmonary tuberculosis patients with diabetes mellitus
Authors: Wu Lianpeng , Chen Na , Xia Dandan , Jiang Xiangao 
Published: January 2025

ObjectiveThis study aimed to investigate the risk factors for multidrug resistance (MDR) in patients with pulmonary tuberculosis (PTB) and diabetes mellitus (DM), including those with and without prior TB treatment.MethodsA retrospective study was conducted from 1 January 2021, to 31 December 2023, at Wenzhou Central Hospital. Patients diagnosed with PTB and DM were included, with multidrug-resistant tuberculosis (MDR-TB) defined as resistance to at least rifampicin and isoniazid. Data on demographics, clinical symptoms, laboratory tests, and treatment history were collected. Multivariate logistic regression analysis was used to identify independent risk factors for MDR, and receiver operating characteristic (ROC) curves were constructed to evaluate the predictive value of these factors.ResultsA total of 318 patients were analyzed, with 253 in the non-MDR group and 65 in the MDR group. Significant independent predictors of MDR included a history of TB treatment, smoking, and elevated hemoglobin A1c (HbA1c) levels. ROC curve analysis showed that the combination of TB treatment history, smoking history, and HbA1c levels had an area under the curve (AUC) of 0.809, with 64.62% sensitivity and 82.61% specificity. In patients without prior TB treatment, smoking history and HbA1c were identified as independent risk factors, with an AUC of 0.771 for their combination. For patients with prior TB treatment, place of residence and pulmonary cavity were independent predictors, with an AUC of 0.802 for their combination.ConclusionThis study highlights the importance of smoking history, HbA1c levels, place of residence, and pulmonary cavity as risk factors for MDR in PTB and DM patients. Early identification of these risk factors can aid in the timely diagnosis and treatment of MDR-TB, potentially reducing its burden. Further research is needed to develop targeted interventions based on these findings.
The visuomotor synchronization immersive virtual reality of a depression avatar in a stigma context experience mobilizes the fronto-parietal cortex and anterior insula
Authors: dos Santos Kawata Kelssy Hitomi , Lem Wey Guan , Ono Koki , Oyama Hiroshi 
Published: January 2025

IntroductionThe gradual synchronization of the movement of one’s real hand with a virtual one can effectively induce a sense of embodiment (SoE) with an avatar with depression. Although neuroimaging studies have explored the neural correlates of some SoE subcomponents of visuomotor synchronization, the neural correlates of individual differences in SoE and how humans acquire virtual body representations through SoE subcomponents remain to be investigated.MethodsHere, we used the right hand of a virtual patient with depression in immersive virtual reality (IVR) to induce SoE in participants and measured whole brain activity using functional magnetic resonance imaging (fMRI). Participants were instructed to listen to the audio recording of the IVR experience and visualize movements during the fMRI scan. fMRI data were acquired before and immediately after the visuomotor synchronization IVR experience (target condition) or an asynchronized video experience (control condition), followed by embodiment measures related to the two types of experiences.ResultsAll five subcomponents of SoE (sense of ownership, sense of agency, sense of localization, appearance, and response to stimuli) were significantly increased during the visuomotor synchronization IVR experience compared with the asynchronized video experience. A significant negative effect of the SoE score was identified in the frontoparietal and anterior insula only for the visuomotor synchronization IVR experience of guiding the virtual right hand of the avatar with depression, implicating interoceptive and multisensory integration.DiscussionWe demonstrated that all five subcomponents of the SoE were present, and that decreased activity in the frontoparietal and anterior insula were crucial brain regions for the virtual human body to be perceived as one’s own body and promote conscious feelings of embodiment.