bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studies of 50 Hz circularly polarized magnetic fields of up to 350 μT on reproduction and embryo‐fetal development in rats: Exposure during organogenesis or during preimplantation
Authors: Negishi Tadashi , Imai Setsuo , Itabashi Masafumi , Nishimura Izumi , Sasano Takao 
Published: October 2002

AbstractGroups of mated female Sprague–Dawley rats were simultaneously exposed to 0 (sham exposed), 7, 70, or 350 μT (rms) circularly polarized 50 Hz magnetic fields (MF) for 22 h/day on gestational day 8–15, the period of rat fetal organogenesis (organogenesis study) or from day 0 to day 7 of gestation, the rat preimplantation period (preimplantation study). Developmental toxicity was assessed on gestational day 20. Identical experiments were repeated to confirm reproducibility of both studies. In both studies, statistically significant differences between exposed and sham exposed animals were observed in several measured parameters; however, these differences only appeared in one, but not both replicate experiments and generally at only an isolated exposure level. Because these differences were not reproducible and did not show a dose response relationship, they were not considered related to MF exposure. In the organogenesis study, lower kidney weights of dams were seen at 70 and 350 μT in Experiment 1. Lower dam liver weights and lower mean body weights of viable female and male fetuses were seen at 70 μT in Experiment 2. Otherwise, there were no differences in these parameters or in group means for fetal loss after implantation, number of viable fetuses, fetal body weight and sex ratio, incidences of external, visceral, and skeletal abnormalities or variations, or tissue abnormalities after histopathological examination. In the preimplantation study, dam health and indices for reproduction and embryo‐fetal development, including pre or postimplantation loss, number and body weight of live fetuses, and sex ratio, external, skeletal abnormalities and variations, and skeletal ossification did not differ. Dam inorganic phosphorous concentration at 350 μT was elevated in one experiment and depressed in another. In one experiment, visceral abnormalities, primarily thymic remnant in neck and accessory liver lobe, were increased in the 7 μT group. Based on these results from two studies, we conclude that circularly polarized 50 Hz MF exposure of up to 350 μT during the fetal organogenesis or during the preimplantation period does not affect reproduction and embryo‐fetal development in Sprague–Dawley rats. Bioelectromagnetics 23:369–389, 2002. © 2002 Wiley‐Liss, Inc.
Potential motion related bias in the worn dosimeter measurements of two childhood leukemia studies
Authors: Leeper Ed , Wertheimer Nancy 
Published: October 2002

AbstractTime averaged field measurements produced by a Positron™ dosimeter worn by study subjects was the primary method of exposure evaluation in two Canadian studies of childhood leukemia and AC magnetic field exposure. Statistically significant but mutually contradictory results obtained in the two studies, done in different locales but under similar study conditions, have not been explained. This report examines operational features of the Positron meter, including an unanticipated sensitivity to wearer motion. If the convalescent cases studied were less active than their healthy controls, as one might expect, then the meter's characteristic responses to motion, particularly as they would affect case‐control distributions above and below the different referent group cutpoints used in the two studies, could help to explain both the unexpected inverse risks reported in the larger study and the unusually high risks reported in the smaller study. Bioelectromagnetics 23:390–397, 2002. © 2002 Wiley‐Liss, Inc.
Importance of mutant position in Ramachandran plot for predicting protein stability of surface mutations
Authors: Gromiha M. Michael , Oobatake Motohisa , Kono Hidetoshi , Uedaira Hatsuho , Sarai Akinori 
Published: October 2002

AbstractUnderstanding the mechanisms by which mutations affect protein stability is one of the most important problems in molecular biology. In this work, we analyzed the relationship between changes in protein stability caused by surface mutations and changes in 49 physicochemical, energetic, and conformational properties of amino acid residues. We found that the hydration entropy was the major contributor to the stability of surface mutations in helical segments; other properties responsible for size and volume of molecule also correlated significantly with stability. Classification of coil mutations based on their locations in the (ϕ–ψ) map improved the correlation significantly, demonstrating the existence of a relationship between stability and strain energy, which indicates that the role of strain energy is very important for the stability of surface mutations. We observed that the inclusion of sequence and structural information raised the correlation, indicating the influence of surrounding residues on the stability of surface mutations. Further, we examined the previously reported “inverse relationship” between stability and hydrophobicity, and observed that the inverse hydrophobic effect was generally applicable only to coil mutations. The present study leads to a simple method for predicting protein stability changes caused by amino acid substitutions, which will be useful for protein engineering in designing novel proteins with increased stability and altered function. © 2002 Wiley Periodicals, Inc. Biopolymers 64: 210–220, 2002
Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris
Authors: Hong Feng , Meinander Nina Q. , Jönsson Leif J. 
Published: October 2002

AbstractImproved expression of recombinant laccase by Pichia pastoris carrying the lcc1 cDNA isolated from Trametes versicolor was achieved by optimization of the cultivation conditions in a fermentor equipped with a methanol sensor system. The results indicated that the activity obtained in fermentor cultivations was at least 7 times higher than in shake‐flask cultures. Three different strategies for fermentor cultivations were compared: A (30°C, 1.0% methanol), B (20°C, 1.0% methanol), and C (20°C, 0.5% methanol). The laccase activity, particularly the specific activity, could be improved by decreasing the cultivation temperature. The mechanisms behind the temperature effect on the laccase activity may be ascribed to poor stability, release of more proteases from dead cells, and folding problems at higher temperature. The results showed that the methanol concentration had a marked effect on the production of active heterologous laccase. A fivefold higher volumetric laccase activity was obtained when the methanol concentration was kept at 0.5% instead of 1.0%. The detrimental effect of methanol on the production of recombinant laccase may be attributed to lower laccase stability, a higher proteolytic activity, and folding problems due to higher growth rate at 1.0% methanol. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 438–449, 2002.
Balancing GFP reporter plasmid quantity in large‐scale transient transfections for recombinant anti‐human Rhesus‐D IgG1 synthesis
Authors: Pick H. M. , Meissner P. , Preuss A. K. , Tromba P. , Vogel H. , Wurm F. M. 
Published: October 2002

AbstractUsing transient expression, high amounts (>20 mg/mL) of secreted anti‐human Rhesus‐D IgG1 were produced in a suspension‐adapted HEK293 EBNA cell line (Meissner et al., Biotechnol Bioeng 75: 197–203, 2001). Time of harvest was 3 days after transfection. For the estimation of transfection efficiencies, we routinely co‐transfected EGFP reporter DNA. At higher reporter plasmid concentrations, >2% of total transfecting plasmid DNA, a substantial reduction of recombinant antibody synthesis, was observed. This phenomenon was investigated in detail by co‐expressing various green fluorescent protein (GFP) reporter constructs, which were targeted at different subcellular locations. Enhanced and humanized GFPs targeted to either the endoplasmic reticulum, the cytosol, or the nucleus reduced recombinant antibody production by 30 to 40% when present at higher concentrations in the transfection solution. The most severe effects were observed when the co‐transfected EGFP was targeted to the endoplasmic reticulum, leading to a reduction of up to 80% in the presence of only 5% of reporter DNA. Interestingly, one nuclear‐targeted GFP variant that was not codon optimized for expression in human cell lines could be added, to up to almost half of the total amount of transfecting DNA, without adverse effect on antibody production. Although the minimum amount of this reporter DNA needed for fluorescence reading was 10 times higher than for the other variants, it provided a much broader quantity range within which the transfection process could be studied without being negatively affected. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 595–601, 2002.
Late aortic dislocation of a stent following stent angioplasty for ostial renal artery stenosis
Authors: Zeller Thomas , Büttner Heinz‐Joachim , Lorenz Hans‐Martin , Frank Ulrich , Müller Christian , Bürgelin Karlheinz , Roskamm Helmut 
Published: October 2002

AbstractA patient with left RAS was treated by stent angioplasty followed by a multivessel percutaneous coronary intervention. Six months later, an aortic dislocation of the stent was diagnosed. The fully expanded stent was caught with a balloon catheter and fixed in the left external iliac artery. Stent migration after initially successful stent angioplasty for RAS is possible. Fully expanded, dislocated balloon‐expandable stents can be secured by implanting them into the iliac artery. Cathet Cardiovasc Intervent 2002;56:416–420. © 2002 Wiley‐Liss, Inc.
Stent deployment within a guide catheter aids removal of a fractured buddy wire
Authors: Prasan Ananth , Brieger David , Adams Mark R. , Bailey Brian 
Published: October 2002

AbstractWe report a case of fractured buddy wire that was successfully removed by deploying a stent within the guide catheter, trapping the fractured segment of wire between stent and endoluminal surface of the guide catheter. This technique provides an alternative to either percutaneous snare or surgical intervention. Cathet Cardiovasc Intervent 2002;56:212–214. © 2002 Wiley‐Liss, Inc.
Usefulness of hydrophilic coating on arterial sheath introducer in transradial coronary intervention
Authors: Saito Shigeru , Tanaka Shinji , Hiroe Yoshitaka , Miyashita Yusuke , Takahashi Saeko , Satake Shutaro , Tanaka Kazushi , Yamamoto Masanobu 
Published: October 2002

AbstractRadial artery spasm is one of the major problems during transradial coronary intervention (TRI). The sheath introducer with hydrophilic coating may reduce the incidence of spasm and reduce the difficulty in removing it from the radial artery under the situation of spasm artery spasm. After we compared the friction resistance between the sheath introducer with hydrophilic coating and that without coating (nine samples each) in vitro, the sheath introducers with and without hydrophilic coating were randomly used in 37 and 36 patients, respectively, who underwent elective TRI with a 6 Fr introducer sheath. Hydrophilic coating of sheath introducer reduced friction resistance by 70% (P < 0.00001) in in vitro model and facilitated sheath removal after finishing TRI (P = 0.0003). Hydrophilic coating of sheath introducer is useful in TRI. Cathet Cardiovasc Intervent 2002;56:328–332. © 2002 Wiley‐Liss, Inc.
Endovascular stent placement for venous obstruction after cardiac transplantation in children and young adults
Authors: Jayakumar Anitha , Hsu Daphne T. , Hellenbrand William E. , Pass Robert H. 
Published: October 2002

AbstractSurvival following cardiac transplantation in children and adults, including the group with complex congenital heart disease, has improved over the last decade secondary to medical and surgical advances in management. There have been rare reports of superior vena cava obstruction at anastomotic sites following transplantation. In patients following heart transplantation, venous stenosis can limit the ability to perform endomyocardial biopsies. We reviewed our experience in three patients who underwent cardiac transplantation and developed significant venous stenosis requiring intervention. All three were successfully treated by transcatheter implantation of endovascular stents. Endovascular stent implantation for venous obstruction in patients following cardiac transplantation was safe and effective, allowing improved ease of catheterization for future posttransplantation monitoring and surveillance. Cathet Cardiovasc Intervent 2002;56:383–386. © 2002 Wiley‐Liss, Inc.
Rotational ablation and stent placement for severe calcific coronary artery stenosis after Kawasaki disease
Authors: Peters Thomas F. , Parikh Sanjay R. , Pinkerton Cass A. 
Published: October 2002

AbstractWe report on a 5‐year‐old child who had an episode of Kawasaki disease with giant coronary artery aneurysms at the age of 4 months. Surveillance coronary angiography showed severe calcific stenosis in the proximal left anterior descending artery. Balloon angioplasty failed to resolve the obstruction. Rotational ablation was therefore performed. Surveillance angiogram performed 6 months after rotational ablation showed critical restenosis. Rotational ablation was therefore repeated, followed by stent placement. To the best of our knowledge, this is the youngest child who has undergone coronary stenting after Kawasaki disease. Cathet Cardiovasc Intervent 2002;56:549–552. © 2002 Wiley‐Liss, Inc.
Overcoming vascular anatomic challenges to cardiac catheterization by the radial artery approach: Specific techniques to improve success
Authors: Esente Paolo , Giambartolomei Alessandro , Simons Alan J. , Levy Carlton , Caputo Ronald P. 
Published: October 2002

AbstractAnatomical variations in the peripheral vasculature can result in decreased procedural success rates for cardiac catheterization performed through the radial artery approach. We describe four categories of vascular challenges encountered in our catheterization laboratory: severe spasm, severe tortuosity, vascular stenosis, and congenital anatomical variations (e.g., accessory radial artery, radioulnar loop). For each situation, we provide a case report illustrating techniques that allowed for successful completion of the case. Cathet Cardiovasc Intervent 2002;56:207–211. © 2002 Wiley‐Liss, Inc.
Distal emboli protection in patients undergoing percutaneous coronary intervention after a recent myocardial infarction
Authors: Kalaria Vijay G. , Rouch Carl , Bourdillon Patrick D. , Breall Jeffrey A. 
Published: October 2002

AbstractDevices to prevent distal atheroembolization are an important addition to the armamentarium of an interventional cardiologist. We report the use of PercuSurge, a distal balloon occlusion device, in two patients with recent myocardial infarction of differing etiologies. The first case is a patient with an old degenerated vein graft with thrombotic occlusion of greater than 48‐hr duration and the second case is a patient with an embolic myocardial infarction in a native coronary artery. In both cases, significant thromboembolic debris was aspirated with technical success and no complications. These cases illustrate use of the PercuSurge device in patients after a recent myocardial infarction for preserving distal myocardial perfusion and microvascular integrity. Cathet Cardiovasc Intervent 2002;57:54–60. © 2002 Wiley‐Liss, Inc.
First closure of a large patent ductus arteriosus in an infant with an angulated nitinol plug
Authors: Ewert Peter , Kretschmar Oliver , Nuernberg Jan H. , Nagdyman Nicole , Lange Peter E. 
Published: October 2002

AbstractWe report the first human implantation of an angulated plug in a 5 mm PDA in a child of 11 kg. Implanted through a 5 Fr sheath, the plug showed excellent anatomical alignment. Complete occlusion was documented after 6 hr. This device will extend the limits for interventional closure of PDA. Cathet Cardiovasc Intervent 2002;57:88–91. © 2002 Wiley‐Liss, Inc.
Pulmonary valvuloplasty in a case of vascular Ehlers‐Danlos syndrome
Authors: Tsai‐Goodman Beverly , Martin Robin P. , Pope F. Michael , Stuart A. Graham 
Published: October 2002

AbstractA 15‐year‐old girl with known Ehlers‐Danlos syndrome (EDS) type IV presented with severe pulmonary valve stenosis requiring intervention. We describe successful pulmonary valvuloplasty using a 90% balloon/annulus angioplasty balloon. The procedure was complicated by early restenosis suggesting an abnormal scarring response in EDS IV. Cathet Cardiovasc Intervent 2002;57:92–94. © 2002 Wiley‐Liss, Inc.
Preferential enrichment: Full crystallographic analysis of the unusual phenomenon in the mixed crystals' version
Authors: Takahashi Hiroki , Tamura Rui , Fujimoto Daisuke , Lepp Zsolt , Kobayashi Kimiko , Ushio Takanori 
Published: October 2002

AbstractFull characterization of the crystal structures of the racemate, nonracemate (20% ee), and pure enantiomer of [2‐[4‐(3‐ethoxy‐2‐hydroxypropoxy)phenylcarbamoyl]ethyl]trimethylammonium p‐bromobenzenesulfonate (NBMe3), which has successfully shown an unusual enantiomeric resolution phenomenon, “preferential enrichment,” was achieved by means of X‐ray crystallographic analysis and construction of the binary melting point phase diagram. The crystalline nature of the racemate is not a racemic compound but a fairly ordered mixed crystal composed of the two enantiomers. The crystal structure of the nonracemate (20% ee) is virtually identical with that of the racemate and similar to that of the pure enantiomer. The binary melting point phase diagram of NBMe3 is consistent with the nature of a mixed crystal composed of the two enantiomers. Chirality 14:541–547, 2002. © 2002 Wiley‐Liss, Inc.
Asymmetric autocatalysis and its application to chiral discrimination
Authors: Soai Kenso , Sato Itaru 
Published: October 2002

AbstractChiral pyrimidyl, quinolyl, and pyridyl alkanols act as asymmetric autocatalysts with significant amplification of enantiomeric excess (ee) in the enantioselective addition of diisopropylzinc to pyrimidine‐5‐, quinoline‐3‐, and pyridine‐3‐carbaldehydes, respectively. 2‐Alkynyl‐5‐pyrimidyl alkanol with as low as 0.6% ee automultiplies during the consecutive asymmetric autocatalysis with increasing ee to as high as >99.5%. Asymmetric autocatalysis is applied to chiral discrimination of organic compounds. In the presence of methyl mandelate or 2‐butanol with very low ee's (0.05–0.1%) as chiral initiators, the reaction between pyrimidine‐5‐carbaldehyde and diisopropylzinc affords pyrimidyl alkanol with higher ee's with the correlated absolute configurations to those of the chiral initiators. Chirality of amino acids (such as leucine) and helicenes with very low ee's are also discriminated by asymmetric autocatalysis, affording pyrimidyl alkanol with very high ee's. Asymmetric autocatalysis also discriminates the chirality of primary alcohols‐α‐d, monosubstituted [2.2]paracyclophanes and octahedral cobalt complex with achiral ligands of which the chirality is due to the topology of coordination of the achiral ligand. Even the chirality of inorganic crystals such as quartz and sodium chlorate is discriminated by asymmetric autocatalysis of pyrimidyl alkanol. Thus, asymmetric autocatalysis provides a unique method for the discrimination of chiral compounds and crystals. Chirality 14:548–554, 2002. © 2002 Wiley‐Liss, Inc.
Assignment of absolute configuration of chiral carboxylic acids via exciton‐coupled CD treatment: 4‐phenylthioproline as a case study
Authors: Di Bari Lorenzo , Mannucci Serena , Pescitelli Gennaro , Salvadori Piero 
Published: October 2002

AbstractThe 4‐hydroxybenzoate chromophore was used as an exciton reporter group for the assignment of the absolute configuration of a chiral carboxylic acid of pharmaceutical interest, (−)‐(2S,4S)‐4‐phenylthioproline, precursor of the ACE‐inhibitor zofenopril. The nondegenerate coupling with the preexisting phenylthio chromophore was observed. A detailed conformational analysis, accomplished by means of 1H‐NMR NOESY and semiempirical PM3 computational methods, and quantitative CD calculations were necessary to substantiate the assignment based on a weak experimental couplet. Chirality 14:611–617, 2002. © 2002 Wiley‐Liss, Inc.
Emerging methods for the rapid determination of enantiomeric excess
Authors: Finn M.G. 
Published: October 2002

AbstractMethods for enantiomeric excess determination using a variety of spectroscopic techniques are summarized. Particular attention is paid to techniques that have promise for application to problems of combinatorial catalyst discovery but have not yet been so employed. Chirality 14:534–540, 2002. © 2002 Wiley‐Liss, Inc.
Arrest of cell cycle progression during first interphase in murine zygotes microinjected with anti‐PCM‐1 antibodies
Authors: Balczon R. , Simerly C. , Takahashi D. , Schatten G. 
Published: October 2002

AbstractTo investigate the function of the centrosome protein PCM‐1, antibodies against PCM‐1 were microinjected into either germinal vesicle stage meiotic oocytes or fertilized mouse eggs, and cell cycle progression events (i.e., microtubule assembly, chromosome and centrosome organization, meiotic maturation) were assayed. These studies determined that microinjected PCM‐1 antibodies arrested cell cycle progression, with anti‐PCM‐1 arresting fertilized eggs at the pronucleate stage when injected during G1. Analysis of the injected eggs determined that centrosome disruption and microtubule cytaster disorganization accompanied the cell cycle arrest. Anti‐PCM‐1 blocked neither pronuclear centration, completion of mitosis when microinjected into zygotes at G2, nor meiotic maturation when microinjected into immature oocytes. These results identify a novel role for PCM‐ 1 in cell cycle regulation, and indicate that PCM‐1 must fulfill an essential function for cells to complete interphase. Cell Motil. Cytoskeleton 52:183–192, 2002. © 2002 Wiley‐Liss, Inc.