bluuebunny/crossref_metadata_2025_split huggingface dataset:
The spatial dynamics of immune response upon virus infection through hybrid dynamical computational model
Authors: Cai Yanan , Zhao Zhongrui , Zhuge Changjing 
Published: November 2023

IntroductionThe immune responses play important roles in the course of disease initiation and progression upon virus infection such as SARS-CoV-2. As the tissues consist of spatial structures, the spatial dynamics of immune responses upon viral infection are essential to the outcome of infection.MethodsA hybrid computational model based on cellular automata coupled with partial differential equations is developed to simulate the spatial patterns and dynamics of the immune responses of tissue upon virus infection with several different immune movement modes.ResultsVarious patterns of the distribution of virus particles under different immune strengths and movement modes of immune cells are obtained through the computational models. The results also reveal that the directed immune cell wandering model has a better immunization effect. Several other characteristics, such as the peak level of virus density and onset time and the onset of the diseases, are also checked with different immune and physiological conditions, for example, different immune clearance strengths, and different cell-to-cell transmission rates. Furthermore, by the Lasso analysis, it is identified that the three main parameters had the most impact on the rate of onset time of disease. It is also shown that the cell-to-cell transmission rate has a significant effect and is more important for controlling the diseases than those for the cell-free virus given that the faster cell-to-cell transmission than cell-free transmission the rate of virus release is low.DiscussionOur model simulates the process of viral and immune response interactions in the alveola repithelial tissues of infected individuals, providing insights into the viral propagation of viruses in two dimensions as well as the influence of immune response patterns and key factors on the course of infection.
Genetic and immunological insights into COVID-19 with acute myocardial infarction: integrated analysis of mendelian randomization, transcriptomics, and clinical samples
Authors: Zheng Zequn , Zhou Yueran , Song Yongfei , Ying Pengxiang , Tan Xuerui 
Published: November 2023

BackgroundGlobally, most deaths result from cardiovascular diseases, particularly ischemic heart disease. COVID-19 affects the heart, worsening existing heart conditions and causing myocardial injury. The mechanistic link between COVID-19 and acute myocardial infarction (AMI) is still being investigated to elucidate the underlying molecular perspectives.MethodsGenetic risk assessment was conducted using two-sample Mendelian randomization (TSMR) to determine the causality between COVID-19 and AMI. Weighted gene co-expression network analysis (WGCNA) and machine learning were used to discover and validate shared hub genes for the two diseases using bulk RNA sequencing (RNA-seq) datasets. Additionally, gene set enrichment analysis (GSEA) and single-cell RNA-seq (scRNA-seq) analyses were performed to characterize immune cell infiltration, communication, and immune correlation of the hub genes. To validate the findings, the expression patterns of hub genes were confirmed in clinical blood samples collected from COVID-19 patients with AMI.ResultsTSMR did not find evidence supporting a causal association between COVID-19 or severe COVID-19 and AMI. In the bulk RNA-seq discovery cohorts for both COVID-19 and AMI, WGCNA’s intersection analysis and machine learning identified TLR4 and ABCA1 as significant hub genes, demonstrating high diagnostic and predictive value in the RNA-seq validation cohort. Single-gene GSEA and single-sample GSEA (ssGSEA) revealed immune and inflammatory roles for TLR4 and ABCA1, linked to various immune cell infiltrations. Furthermore, scRNA-seq analysis unveiled significant immune dysregulation in COVID-19 patients, characterized by altered immune cell proportions, phenotypic shifts, enhanced cell-cell communication, and elevated TLR4 and ABCA1 in CD16 monocytes. Lastly, the increased expression of TLR4, but not ABCA1, was validated in clinical blood samples from COVID-19 patients with AMI.ConclusionNo genetic causal link between COVID-19 and AMI and dysregulated TLR4 and ABCA1 may be responsible for the development of immune and inflammatory responses in COVID-19 patients with AMI.
Molecular epidemiology and pathogenomics of extended-spectrum beta-lactamase producing- Escherichia coli and - Klebsiella pneumoniae isolates from bulk tank milk in Tennessee, USA
Authors: Gelalcha Benti D. , Mohammed Ruwaa I. , Gelgie Aga E. , Kerro Dego Oudessa 
Published: November 2023

IntroductionThe rise in extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in dairy cattle farms poses a risk to human health as they can spread to humans through the food chain, including raw milk. This study was designed to determine the status, antimicrobial resistance, and pathogenic potential of ESBL-producing -E. coli and -Klebsiella spp. isolates from bulk tank milk (BTM).MethodsThirty-three BTM samples were collected from 17 dairy farms and screened for ESBL-E. coli and -Klebsiella spp. on CHROMagar ESBL plates. All isolates were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and subjected to antimicrobial susceptibility testing and whole genome sequencing (WGS).ResultsTen presumptive ESBL-producing bacteria, eight E. coli, and two K. pneumoniae were isolated. The prevalence of ESBL-E. coli and -K. pneumoniae in BTM was 21.2% and 6.1%, respectively. ESBL-E. coli were detected in 41.2% of the study farms. Seven of the ESBL-E. coli isolates were multidrug resistant (MDR). The two ESBL-producing K. pneumoniae isolates were resistant to ceftriaxone. Seven ESBL-E. coli strains carry the blaCTX-M gene, and five of them co-harbored blaTEM-1. ESBL-E. coli co-harbored blaCTX-M with other resistance genes, including qnrB19, tet(A), aadA1, aph(3’’)-Ib, aph(6)-Id), floR, sul2, and chromosomal mutations (gyrA, gyrB, parC, parE, and pmrB). Most E. coli resistance genes were associated with mobile genetic elements, mainly plasmids. Six sequence types (STs) of E. coli were detected. All ESBL-E. coli were predicted to be pathogenic to humans. Four STs (three ST10 and ST69) were high-risk clones of E. coli. Up to 40 virulence markers were detected in all E. coli isolates. One of the K. pneumoniae was ST867; the other was novel strain. K. pneumoniae isolates carried three types of beta-lactamase genes (blaCTX-M, blaTEM-1 and blaSHV). The novel K. pneumoniae ST also carried a novel IncFII(K) plasmid ST.ConclusionDetection of high-risk clones of MDR ESBL-E. coli and ESBL-K. pneumoniae in BTM indicates that raw milk could be a reservoir of potentially zoonotic ESBL-E. coli and -K. pneumoniae.
Fronto-parietal theta high-definition transcranial alternating current stimulation may modulate working memory under postural control conditions in young healthy adults
Authors: Xiao Yanwen , Zhou Junhong , Zhou Rong , Liu Yu , Lü Jiaojiao , Huang Lingyan 
Published: November 2023

ObjectsThis study aimed to investigate the immediate effects of fronto-parietal θ HD-tACS on a dual task of working memory-postural control.MethodsIn this within-subject cross-over pilot study, we assessed the effects of 20 min of 6 Hz-tACS targeting both the left dorsolateral prefrontal cortex (lDLPFC) and posterior parietal cortex (PPC) in 20 healthy adults (age: 21.6 ± 1.3 years). During each session, single- and dual-task behavioral tests (working memory single-task, static tandem standing, and a dual-task of working memory-postural control) and closed-eye resting-state EEG were assessed before and immediately after stimulation.ResultsWithin the tACS group, we found a 5.3% significant decrease in working memory response time under the dual-task following tACS (t = −3.157, p = 0.005, Cohen’s d = 0.742); phase synchronization analysis revealed a significant increase in the phase locking value (PLV) of θ band between F3 and P3 after tACS (p = 0.010, Cohen’s d = 0.637). Correlation analyses revealed a significant correlation between increased rs-EEG θ power in the F3 and P3 channels and faster reaction time (r = −0.515, p = 0.02; r = −0.483, p = 0.031, respectively) in the dual-task working memory task after tACS. However, no differences were observed on either upright postural control performance or rs-EEG results (p-values &lt;0.05).ConclusionFronto-parietal θ HD-tACS has the potential of being a neuromodulatory tool for improving working memory performance in dual-task situations, but its effect on the modulation of concurrently performed postural control tasks requires further investigation.
Digitalisation in the WFP fresh food voucher programme: a pilot study from rural Amhara region, Ethiopia
Authors: Dad Fazal , Dibari Filippo , Kebede Aweke , Lefu Emma , Ndumiyana Tafara , Butaumocho Blessing 
Published: November 2023

IntroductionMalnutrition continues to pose a major challenge to human well-being around the world. In Ethiopia, 39% of children &lt;5 years are stunted, with peaks in northern regions of the country such as Amhara (54.8%). Very few (2%) children in the region achieve the minimum dietary diversity and only a minority (27%) belong to households that can afford a nutritious diet. To tackle the high stunting rate, diets high in fruits and vegetables are widely recommended to improve dietary diversity. Programmes leveraging fresh food vouchers can be used to support vulnerable groups with malnutrition and limited affordability. Cash-based transfer (CBT) programmes have repeatedly been shown to improve child growth and increase household food security and dietary diversity. This study is part of the World Food Programme (WFP) intervention regarding a stunting reduction rural programme of restricted CBT for improving dietary diversity in households with children under 2 years of age and pregnant and lactating women.MethodsA community- based pilot study to assess the itemised foods purchased by beneficiaries was conducted in the four most accessible woredas of the Amhara region of Ethiopia. A total of 556 beneficiaries and 12 active retailers were selected randomly from 10 rural markets in the targeted woredas. A point of sale (POS) system was used to collect the itemised food prices and amounts of food procured by the beneficiaries.Results and DiscussionApproximately 51, 35, and 15% of the beneficiaries purchased vegetables, fruits, and eggs, respectively. Prices, taste preferences of children, and shelf life determine the purchase of certain food items. The average food expenditure was 49 Ethiopian Birr (ETB; US$ 0.94), representing the 63 and 37% daily and monthly requirements, respectively, for affordability. The higher increase in prices of some food items might be due to their seasonality. Almost half of the Fresh Food Voucher (FFV) beneficiaries were purchasing and consuming vegetables. The finding indicates that the WFP fresh food voucher programme contributes 63% (49 ETB, US$ 0.94) and 37% (837 ETB, US$ 16.1) of the daily and monthly needs of affordability, respectively, for a diversified nutritious diet. The use of Unstructured Supplementary Service Data (USSD) technology in the WFP digital voucher under the Fresh Food Voucher (FFV) project was effective at collecting itemised prices of foods purchased by the beneficiaries. The point of sale (POS)1 system can be scaled up under the Fresh Food Voucher (FFV) programme so that the digital voucher can contain the itemised food prices. Timely data from the point of sale could be used for timely Social Behaviour Change Communication (SBCC) development to improve dietary diversity.
What are the benefits of cultivating self-compassion in adults with low back pain? A systematic review
Authors: Greff Ballejos Kellen , Calvetti Prisla Ücker , Schaab Bruno Luis , Reppold Caroline Tozzi 
Published: November 2023

IntroductionLow back pain is one of the most prevalent public health problems in the world, generating psychosocial impacts on quality of life and a high demand for medical care. Self-compassion may be beneficial for low back pain control, however, studies in the area are scarce. Therefore, this systematic review aimed to investigate the benefits of self-compassion-related interventions on low back pain and mental health in adults.MethodsThe review protocol was registered in PROSPERO and the method was performed according to the PRISMA guidelines. Searches were conducted using the keywords “self-compassion” and “low back pain” in Portuguese, English, and Spanish in the following databases: PubMed, LILACS, SciELO, PePSIC, PsycInfo, Embase, Scopus, Web of Science, and Cochrane. Additional searches were also conducted through the references of the included studies.ResultsThirty-three articles were identified and analyzed by two independent reviewers using Rayyan. Four of these studies were included. RoB 2 was used to assess the risk of bias of each study. The main findings suggest that self-compassion-related interventions demonstrate benefits in the treatment of low back pain, as well as reduction in pain intensity, psychological stress, and improvement of pain acceptance.DiscussionHowever, these positive data must be analyzed carefully, as only two studies presented a low risk of bias. Despite growing interest in this field, more research self-compassion-related interventions for low back pain are suggested, since biopsychosocial aspects associated with low back pain can impact the outcome of treatment.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier (CRD42022376341).
Going vertical: examining the rise and impact of contemporary Russian sports cinema
Authors: Crosson Seán 
Published: November 2023

IntroductionWhile Hollywood has been central to the definition and popularisation of the genre internationally, sport cinema also has a significant presence in national cinemas across the world, though less research has been undertaken to date of the place of sport within European cinema and its growing importance in this context. This article will introduce a new database and online research platform (Sport in European Cinema, sportandfilm.eu) recently made publicly available which enables a deeper consideration of the significance of sport in European cinema, including its increasing importance in the Russian context. Indeed the third most commercially successful indigenous film ever released (as of 2023) at the Russian box office is a sports film, Движение вверх (Going Vertical (AKA Three Seconds) (2017).MethodsThis article adopts a mixed-methods approach, combining quantitative and qualitative methods to examine the place of sport in European cinema, including a case study of contemporary Russian sport cinema and its connection with broader political and ideological messaging.ResultsSport cinema has become an increasingly important part of European cinema, both in terms of number of films produced and their impact on European society, as evident in the case study provided of Russian sport cinema.ConclusionThis paper introduces a new research database dedicated to European Sport Cinema and identifies the increasing importance of sport cinema in the European context. It includes a case study of contemporary Russian sport cinema and identifies its role in articulating and affirming existing power structures and ideological hegemony within Russian society, as evident within Going Vertical and further recent Russian sport films in a particularly challenging period of national and international instability and contestation.
Lifetime prevalence of owner-reported medical conditions in the 25 most common dog breeds in the Dog Aging Project pack
Authors: Forsyth Kiersten K. , McCoy Brianah M. , Schmid Sarah M. , Promislow Daniel E. L. , Snyder-Mackler Noah ,   the DAP Consortium
Published: November 2023

IntroductionLarge scale data on the prevalence of diverse medical conditions among dog breeds in the United States are sparse. This cross-sectional study sought to estimate the lifetime prevalence of medical conditions among US dogs and to determine whether purebred dogs have higher lifetime prevalence of specific medical conditions compared to mixed-breed dogs.MethodsUsing owner-reported survey data collected through the Dog Aging Project (DAP) Health and Life Experience Survey for 27,541 companion dogs, we identified the 10 most commonly reported medical conditions in each of the 25 most common dog breeds within the DAP cohort. Lifetime prevalence estimates of these medical conditions were compared between mixed-breed and purebred populations. The frequency of dogs for whom no medical conditions were reported was also assessed within each breed and the overall mixed-breed and purebred populations.ResultsA total of 53 medical conditions comprised the top 10 conditions for the 25 most popular breeds. The number of dogs for whom no medical conditions were reported was significantly different (p = 0.002) between purebred (22.3%) and mixed-breed dogs (20.7%). The medical conditions most frequently reported within the top 10 conditions across breeds were dental calculus (in 24 out of 25 breeds), dog bite (23/25), extracted teeth (21/25), osteoarthritis (15/25), and Giardia (15/25).DiscussionPurebred dogs in the DAP did not show higher lifetime prevalence of medical conditions compared to mixed-breed dogs, and a higher proportion of purebred dogs than mixed-breed dogs had no owner-reported medical conditions. Individual breeds may still show higher lifetime prevalence for specific conditions.
Diaporthe Species on Palms: Molecular Re-Assessment and Species Boundaries Delimitation in the D. arecae Species Complex
Authors: Pereira Diana S. , Hilário Sandra , Gonçalves Micael F. M. , Phillips Alan J. L. 
Published: November 2023

Due to cryptic diversification, phenotypic plasticity and host associations, multilocus phylogenetic analyses have become the most important tool in accurately identifying and circumscribing species in the Diaporthe genus. However, the application of the genealogical concordance criterion has often been overlooked, ultimately leading to an exponential increase in novel Diaporthe spp. Due to the large number of species, many lineages remain poorly understood under the so-called species complexes. For this reason, a robust delimitation of the species boundaries in Diaporthe is still an ongoing challenge. Therefore, the present study aimed to resolve the species boundaries of the Diaporthe arecae species complex (DASC) by implementing an integrative taxonomic approach. The Genealogical Phylogenetic Species Recognition (GCPSR) principle revealed incongruences between the individual gene genealogies. Moreover, the Poisson Tree Processes’ (PTPs) coalescent-based species delimitation models identified three well-delimited subclades represented by the species D. arecae, D. chiangmaiensis and D. smilacicola. These results evidence that all species previously described in the D. arecae subclade are conspecific, which is coherent with the morphological indistinctiveness observed and the absence of reproductive isolation and barriers to gene flow. Thus, 52 Diaporthe spp. are reduced to synonymy under D. arecae. Recent population expansion and the possibility of incomplete lineage sorting suggested that the D. arecae subclade may be considered as ongoing evolving lineages under active divergence and speciation. Hence, the genetic diversity and intraspecific variability of D. arecae in the context of current global climate change and the role of D. arecae as a pathogen on palm trees and other hosts are also discussed. This study illustrates that species in Diaporthe are highly overestimated, and highlights the relevance of applying an integrative taxonomic approach to accurately circumscribe the species boundaries in the genus Diaporthe.
Perspectivas filosóficas en la educación del siglo XXI
Authors: Magallanes Payan José Arturo 
Published: November 2023

El propósito del presente trabajo es identificar y describir las influencias discursivo-hegemónicas en el fenómeno educativo contemporáneo, sus sincronías y disonancias. Para el análisis se parte de las perspectivas del ejercicio de la filosofía que ofrecen varios autores, principalmente la que expone Alain Badiou de sutura filosófica, esto para sintetizar las imbricaciones entre ciencia y política y mostrar cómo constituyen su organización de subjetividades y prácticas al interior del proceso de enseñanza-aprendizaje. Se exponen esas imbricaciones y cómo se articulan los conceptos (competencias, eficacia, éxito), suturas (tecnocracia pospolítica) y tendencias (paradigma sociotécnico y cultura digital) como una manera de explicar y describir como influyen en la lógica educativa actual. Finalmente, se enmarca una posible tendencia que el análisis de estos elementos ofreció, una pedagogía instruccionista en la cual la operatividad exitosa de la información es el factor determinante.
Marketing Strategy for Cibaduyut UKM Craftsmen Shoes During the Covid-19 Pandemic
Authors: Muttaqin Rizqi , Saputera Denny , Dewi Rina Ariyanthi , Moenardy Dwi Fauziansyah , Santoso* Rizal Budi , Alam Gilang Nur 
Published: November 2023

This article aims to explain the marketing strategy for MSME shoemakers in the Cibaduyut area during the Covid-19 pandemic. The purpose of this study was to determine the effect of the Covid-19 pandemic on MSMEs shoe craftsmen in the Cibaduyut area and the efforts of these MSMEs in utilizing digital platforms to maintain their business during the Covid-19 pandemic. The research method uses qualitative methods through interviews and observation as well as literature and data reduction. This research shows the challenges faced by MSMEs in utilizing digital platforms and provides recommendations for improving marketing strategies so that MSMEs can utilize digital platforms more effectively.
Keywords: Cibaduyut; Mix Marketing; MSME; Shoesmakers; Marketing Strategy
Enhancing Employee Performance: Rewards, Training, and Work Ethics at the Education Office of the Bangka Belitung Islands Province
Authors: Yahya Yanda , Hamsani Hamsani , Reniati* Reniati 
Published: November 2023

This research was motivated by the existing condition where employees have not been able to work optimally in a government institution. The study aims to analyze and examine the influence of rewards, training, and work ethic on Employee Performance in the Education Authority of Bangka Belitung Island Province. This research is descriptive in nature. The quantitative research method was employed because the data obtained are numerical and derived from questionnaires, with a total sample size of 64 respondents, determined through a saturated sample method. The analytical model used was multiple linear regression, with t-tests, f-tests, and coefficients of determination. The research results indicate that rewards, training, and work ethic, individually, have a positive and significant impact on the performance of employees in the Education Authority of Bangka Belitung Island Province. Through this research, institutions can review the factors leading to low employee performance in the variables of rewards, training, and work ethic.
Keywords: Reward; Training; Work Ethic; Employee Performance; Education Office