bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring of Macroinvertebrate Species In Sibolga Waters
Authors: Harahap Arman 
Published: February 2025

The waters of sibolga is the waters are very widespread in , this area is densely populated, so there are many community activities that take place such as Transportation, Fisheries, Bathing, sand Mining and other.The purpose of this study is to determine the role of Macroinvertebrate as a bioindicator of water quality in the waters of Sibolga Tapanuli Tengah, as well as determine the quality of water in the flow of the waters Sibolga physical properties, chemical has. The research was conducted from November 2021 to February 2022, the samples taken from the three research stations. From the results obtained Macroinvertebrate in the identification in this study consisted of 3 Phyla of invertebrates namely : Annelids, which consists of 2 classes, Arthropods consisting of 1 class and Molusca, which consists of 1 class. Based on the data of Macroinvertebrate obtained at each research station, then the obtained value of population density, relative density and frequency of presence of that at station I the value of its highest density is Tubifex sp. with the value of the density of the population of 18,519 individual/m2, the density is relatively 25,862% and the frequency of the presence of 55,555%. off at 3. at station 2 the highest density is Tubifex sp. with the value of the density of the population of 18,519 individuals/m2, relative density 25,862% and a frequency of 55,555%. also note that the total score obtained by the method storet, namely -30, this shows that if connected with the raw water quality of class I is then classified in the waters of class III, which means that light polluted. The high total score method storet on the entire research station due to the presence of a variety of activities at each of the stations, settlements, sand mining and other activities. Where these activities produce waste, which in the end increases the content of Phosfat, COD, TSS and BOD5, thus causing the waters of the Waters of Sibolga is light polluted.
Identification of lipid metabolism related immune markers in atherosclerosis through machine learning and experimental analysis
Authors: Chen Hang , Wu Biao , Guan Kunyu , Chen Liang , Chai Kangjie , Ying Maoji , Li Dazhi , Zhao Weicheng 
Published: February 2025

BackgroundAtherosclerosis is a significant contributor to cardiovascular disease, and conventional diagnostic methods frequently fall short in the timely and accurate detection of early-stage atherosclerosis. Abnormal lipid metabolism plays a critical role in the development of atherosclerosis. Consequently, the identification of new diagnostic markers is essential for the precise diagnosis of this condition.MethodThe datasets related to atherosclerosis utilized in this research were obtained from the GEO database (GSE2470, GSE24495, GSE100927 and GSE43292). The ssGSEA technique was first utilized to assess lipid metabolism scores in samples affected by atherosclerosis, thereby aiding in the discovery of important regulatory genes linked to lipid metabolism via WGCNA. Following this, differential expression analysis and functional evaluations were carried out, after which various machine learning approaches were employed to determine significant diagnostic genes for atherosclerosis. A diagnostic model was then developed and validated through several machine learning algorithms. Furthermore, molecular docking studies were conducted to analyze the binding affinity of these key markers with therapeutic agents for atherosclerosis. The ssGSEA technique was also used to measure immune cell scores in atherosclerotic samples, aiding the exploration of the connection between key diagnostic markers and immune cells. Finally, the expression variations of the identified pivotal genes were confirmed through experimental validation.ResultWGCNA identified 302 lipid metabolism-related genes in atherosclerotic samples, and functional analysis revealed that these genes are associated with multiple immune pathways. Through further differential analysis and screening using machine learning algorithms, APLNR, PCDH12, PODXL, SLC40A1, TM4SF18, and TNFRSF25 were identified as key diagnostic genes for atherosclerosis. The diagnostic model we constructed was confirmed to predict the occurrence of atherosclerosis with high accuracy, and molecular docking studies indicated that these six key diagnostic genes have potential as drug targets. Additionally, the ssGSEA algorithm further validated the association of these diagnostic genes with various immune cells. Finally, the expression levels of these six genes were experimentally confirmed.ConclusionOur study introduces novel lipid metabolism-related diagnostic markers for atherosclerosis and emphasizes their potential as immune-related drug targets. This research provides a valuable approach for the predictive diagnosis and targeted therapy of atherosclerosis.
Efficacy and safety of small molecule drugs in the treatment of pityriasis rubra pilaris—A systematic review
Authors: Zhang Xiaofang , Wei Kebo , Song Hongxia , Chen Xi , Yang Jiao , Zhao Jianmei , Jiang Yugu , He Xin 
Published: February 2025

BackgroundPityriasis rubra pilaris is a chronic, scaly, keratotic skin disease, mainly manifested as scaly plaques and keratinized hair follicles. This condition significantly impacts the patient’s quality of life and is considered one of the intractable diseases in dermatology. Currently, no satisfactory clinical treatment options are available for this condition, presenting a considerable challenge for dermatologists. We conducted this systematic evaluation to assess the therapeutic potential of existing small molecule drugs for this disease.ObjectivesTo conduct a systematic review of the existing literature on the use of small molecule drugs for treating pityriasis rubra pilaris and to evaluate their clinical effectiveness and safety.MethodsWe conducted a systematic review of all the literature on small molecule drugs for the treatment of Pityriasis rubra pilaris and searched several databases until November 2024, including PubMed, Embase, Web of Science, and the Cochrane Library.ResultsA total of 16 patients with pityriasis rubra pilaris from 11 publications were included. The small molecule drugs, including apremilast, upadacitinib, abrocitinib, and tofacitinib, demonstrate good efficacy and safety in the treatment of pityriasis rubra pilaris across all ages, particularly in patients who have failed systemic therapy and have a poor response to biological agents. However, the conclusions are limited by the small sample size and need to be further confirmed through large-scale randomized controlled clinical trials.ConclusionSmall molecule drugs demonstrate favorable clinical efficacy and safety in the treatment of refractory pityriasis rubra pilaris, exhibiting a relatively rapid onset and a high safety profile. However, the findings in the literature may be affected by publication bias.
Effects of partial silage replacement with corn stover pellets on the rumen microbiota and serum metabolome of breeding cows
Authors: Jiao Chenyue , Cui Changze , Qi Youpeng , Zhang Meixian , Zhao Pengcheng , Chen Shaopeng , Wang Xiangyan , Hu Jiang , Shi Bingang , Liu Ting , Zhao Zhidong , Zhao Fangfang 
Published: February 2025

IntroductionStraw pellet ration replacing part of silage is of great significance for farmers to save farming costs and solve the lack of feed resources. A comprehensive analysis of rumen microbial and serum metabolite compositions is conducted to promote the development of the modern breeding cows-feeding industry.MethodsIn this study, 18 healthy 2-year-old Simmental breeding cows weighing 550 ± 20 kg were selected and randomly divided into two groups. They were fed under the same feeding conditions for 70 days, of which 8 in the control (CON) group were fed 65% roughage (100% silage) + 35% concentrate, and 10 in the treatment (TRT) group were fed 65% roughage (50% corn stover pellets +50% silage) + 35% concentrate, and milk quality, serum immunity indexes, serum metabolomes, rumen fermentation parameters, rumen Microorganisms.ResultsThe results showed that there was no significant difference in production performance between the two groups of breeding cows fed hay and Corn stover pellet feed (p &lt; 0.05); Immunoglobulin A (IgA) was significantly higher in TRT compared to CON (p &lt; 0.05), and there was no significant difference in Immunoglobulin G (IgG) and Immunoglobulin M (IgM) between the two groups (p &gt; 0.05); a total of 92 differential metabolites were screened out in the serum metabolomics analysis, among them, L-valine, L-leucine, L-arginine, L-cysteine, L-tyrosine, and L-tryptophan were up-regulated; In rumen fermentation parameters there was no significant difference between CON and TRT in rumen pH, rumen ammonia nitrogen (NH3-N) content, rumen Acetic/Propionic concentration (p &gt; 0.05), and the concentration of Acetic, Propionic, butyric and Total volatile fatty acids (TVFA) in CON was significantly lower than that in TRT (p &lt; 0.05). Among the rumen microorganisms, the dominant groups were Thick-walled Firmicutes, Bacteroidota, Prevotella and Ruminalococcus. In the correlation analysis between rumen fermentation parameters and rumen microorganisms, Propionic and TVFA showed a significant positive correlation with Prevotella (p &lt; 0.05), butyric showed a highly significant positive correlation with Prevotella (p &lt; 0.01), and propionic butyric, and TVFA showed a positive correlation with Bacteroides (p &lt; 0.05); L-cysteine was significantly positively correlated with Prevotella and Anaeroplasma (p &lt; 0.05) and Eubaterium in rumen microbial-serum metabolite correlation analysis (p &lt; 0.01).ConclusionThe microbial and metabolomic analyses provide us with essential data support to further provide a scientific basis for breeding cows feeding through the feeding pattern of straw pellets instead of silage, which will help breeding cows farming in future research.
The impact of frequency-specific music stimulation on consciousness in patients with disorders of consciousness
Authors: Yang Haitao , Huang Wenhao , Wen Wei , Long Shoufeng , Zhang Yujie , Chi Xiangfeng , Luo Daihong 
Published: February 2025

ObjectiveThis study aimed to evaluate the effects of frequency-specific music stimulation on the awareness and brain connectivity in patients with disorders of consciousness (DOC).MethodsA total of 25 DOC patients were exposed to auditory stimulation through music at varying frequencies (low: &lt;0.5 Hz, middle: 0.5 Hz–3.5 kHz, high: &gt;3.5 kHz). Brain responses were assessed using Functional Near-Infrared Spectroscopy (fNIRS) to monitor objective markers of brain activity. The analysis focused on effective connectivity and coupling strength (CS) values in response to different frequency stimulations, targeting regions such as the motor and somatosensory cortices.ResultsThe mean age of the patients was 49.4 years, with an average coma duration of 1.96 months. While no significant differences were observed in general brain arousal across different frequency stimuli, notable differences in effective connectivity were identified. High-frequency stimulation resulted in significantly higher CS values in the right primary motor cortex (p &lt; 0.05), while middle-frequency stimulation showed significant effects in the right primary somatosensory cortex (p = 0.016).ConclusionThe findings suggest that middle- and high-frequency music stimulation may enhance effective connectivity in specific brain regions, potentially contributing to the rehabilitation of DOC patients. These results indicate that frequency-specific music could stimulate motor networks and areas associated with autobiographical memory, highlighting its therapeutic potential in promoting awareness in this patient population.
Different factors modulate visceral and subcutaneous fat accumulation in adults: a single-center study in Brazil
Authors: Chagas Camila Lima , da Silva Nadja Fernandes , Rodrigues Isa Galvão , Arcoverde Gabriela Maria Pereira Floro , Ferraz Victoria Domingues , Sobral Filho Dário Celestino , Diniz Alcides da Silva , Pinho Cláudia Porto Sabino , Cabral Poliana Coelho , de Arruda Ilma Kruze Grande 
Published: February 2025

BackgroundAbdominal adipose tissue consists of visceral and subcutaneous fat deposits, each with unique metabolic and functional properties. Identifying the characteristics that influence different obesity phenotypes can support targeted prevention and intervention strategies.ObjectiveTo identify predictive factors associated with visceral and subcutaneous adipose tissue accumulation.MethodsThis is a cross-sectional study including adults of both sexes aged ≥20 years under outpatient care in a public healthcare service in Northeast Brazil. Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured via ultrasound. Anthropometric, clinical, sociodemographic, and behavioral variables were incorporated into the predictive model.ResultsA total of 347 individuals were included. They were median age of 47.0 years (interquartile range: 39.0 to 56.0). Visceral obesity was found in 79.3% of the sample. Adjusted analysis demonstrated that physical inactivity (OR 2.3; 95% CI 1.1–4.7; p = 0.023) and elevated waist circumference (WC) (OR 6.4; 95% CI 2.6–15.8 p &lt; 0.001) were associated with VAT accumulation. Alcohol consumption increased the likelihood of SAT accumulation by 2.2 times (95% CI 1.3–3.7; p = 0.005), while elevated WC raised this likelihood by 4.5 times (95% CI 2.1–9.8; p &lt; 0.001). The VAT/SAT ratio was significantly higher in older adults (OR 5.5; 95%CI 2.0–14.8; p = 0.001), among individuals of Mixed Race and Black, those with lower educational levels (OR 2.4; 95%CI 1.1–5.2; p = 0.028), and in diabetics (OR 2.4; 95%CI 1.2–4.9; p = 0.017).ConclusionDistinct factors influence visceral and subcutaneous obesity. Sedentary behavior emerged as an independent predictor of visceral obesity, while alcohol consumption was associated with a subcutaneous obesity pattern. Diabetes and sociodemographic factors (older age, non-White race, and lower education) were predictive of an elevated VAT/SAT ratio.
Protective effect of dietary phosphorus intake on cardiovascular mortality in asthma: evidence from NHANES 1999–2018
Authors: Yang Shuwen , Chen Haiyan , Xie Congyi , Zhang Ning 
Published: February 2025

BackgroundAsthma is associated with an increased risk of cardiovascular mortality, potentially influenced by dietary phosphorus intake through its effects on inflammation and oxidative stress.MethodsData from 7,539 asthma patients in the National Health and Nutrition Examination Survey (NHANES) 1999–2018 cohort were analyzed using weighted Cox proportional hazards models to estimate hazard ratios (HR) and 95% confidence intervals (CI). Kaplan-Meier survival curves and a nomogram were used to assess survival probabilities and individualized risk, while restricted cubic spline (RCS) analysis evaluated non-linear dose-response relationships. Sensitivity analyses were conducted to test the robustness of the findings.ResultsHigher dietary phosphorus intake was associated with reduced cardiovascular mortality (HR: 0.43; 95% CI: 0.22–0.85 for the highest vs. lowest quartile; p for trend = 0.018). Kaplan-Meier curves showed improved survival with increasing phosphorus intake, a result consistently supported by subgroup analyses. RCS analysis confirmed a non-linear dose-response relationship, identifying a threshold at 1,861.52 mg/day, below which higher phosphorus intake was significantly associated with lower cardiovascular mortality. However, above this threshold, the protective effect diminished. Sensitivity analyses further validated these results.ConclusionElevated dietary phosphorus intake is associated with reduced cardiovascular mortality in asthma patients, suggesting its potential as a dietary intervention.
Does living alone exacerbate depression in older adults?
Authors: Wang Haolin , Sun Bing 
Published: February 2025

IntroductionLiving alone, which has become increasingly common in China, weakens the emotional connections between parents and children—fundamental family functions in Confucianism. This trend has raised concerns regarding depression among older adults living alone. Numerous researchers have evaluated the influence of living alone on depression among older adults in different cultures. However, the consensus has yet to be reached.MethodsThis study adopted a fixed effects model to analyze three sets of data from the China Health and Retirement Longitudinal Study based on different family cultures reflected by living arrangements.ResultsLiving alone reduced depression among older adults by 0.267 (CES-D, 10–40). The effects of living alone on depression among older adults predominantly originate from living close to their children. This lifestyle effectively balances the need for individual independence and the demand for maintaining tight family relationships and reconciles intergenerational family conflict. Differences between rural and urban areas were also identified. This search indicated that living alone improved depression in rural older adults; however, no significant effects were found for urban older adults.DiscussionAs living close to their children is conducive to improving depression among older adults, policymakers are advised to focus on local employment. Special emphasis should be placed on incentivizing migrant workers to return to their rural hometowns for entrepreneurship or employment.
Lower creatinine levels are associated with an increased risk of depression: evidence from the China Health and Retirement Longitudinal Study
Authors: Liu Fajin , Zhong Xiaogang , Wang Cuiting 
Published: February 2025

IntroductionPrevious studies have found that depressive patients tend to have low levels of creatinine; however, the extent to which creatinine levels are associated with depression has been poorly investigated. Therefore, this study aimed to explore the relationship between creatinine levels and depression.MethodsThe participants and follow-up data from the China Health and Retirement Longitudinal Study (CHARLS), as well as metabolomics data from the Metabolite Network of Depression Database (MENDA), were collected. The 10-item Center for Epidemiologic Studies Depression Scale (CESD-10) was used to assess the severity of depression. Spearman correlation analysis, spline regression, and binary logistic regression models were employed to explore the relationship between creatinine levels and depression.ResultsA total of 7,826 participants and 3,886 follow-up participants were included in the CHARLS 2011 and 2015 surveys. Of these, 37.9% (2,966/7,826) and 34.6% (13,44/3,886) of participants experienced depression in CHARLS 2011 and 2015, respectively. The creatinine level was negatively correlated with the total CESD-10 score and dimensions scores, showing an inverse dose–response relationship between creatinine levels and depression. Compared with participants with high creatinine levels, those with middle creatinine levels were associated with a higher risk of depression (OR = 1.22, 95% CI = 1.08–1.38), while participants with low creatinine levels had the highest risk of depression (OR = 1.30, 95% CI = 1.13–1.49) in the fully adjusted model. Similar results were observed in the follow-up data, and the MENDA metabolomics data validated the negative correlation between creatinine levels and the severity of depression.ConclusionLower levels of creatinine were closely associated with a higher risk of depression, and it could serve as a potential marker for identifying individuals at high risk of depression.
Genomic and Transcriptomic Profiling of Bacillus cereus in Milk: Insights into the Sweet Curdling Defect
Authors: Kyritsi Maria , Tsiolas George , Tsoureki Antiopi , Schoretsaniti Vasiliki , Gougouli Maria , Michailidou Sofia , Argiriou Anagnostis 
Published: February 2025

Bacillus cereus sensu lato (B. cereus s.l.) are significant spoilage and pathogenic microorganisms found in various foodstuffs. They are responsible for defects like sweet curdling in milk, which impacts dairy product storage and distribution. Nevertheless, the genetic mechanisms underlying B. cereus-induced sweet curdling remain poorly characterized. In this study, we investigated the genetic and functional basis underlying this phenomenon through whole genome sequencing of the newly isolated B. cereus strain BC46 and transcriptome sequencing at two phases of its growth in milk. Hybrid assembly of Illumina and Nanopore reads resulted in a 5.6 Mb genome with 35.1% GC content, classifying BC46 as B. cereus sensu stricto (B. cereus s.s.) within the panC group IV. Several virulence factors, antimicrobial resistance genes, and cold shock proteins were identified in the genome. A distinct functional profile of BC46 was observed before and after the development of sweet curdling in milk. Genes associated with sporulation, toxin production, hydrolysis, and proteolysis were upregulated in sweet-curdled samples. Our findings highlight potential gene targets that may play an important role in the BC46-induced sweet curdling in milk, enhancing our understanding of its molecular basis and supporting the development of new genetic approaches for early spoilage detection.
A dislipidemia em decorrência do tabagismo e suas consequências no sistema circulatório
Authors: Silva Camila Eduarda Lima , Santos Lorena Alves Cezarino , Júnior Marcus Vinícius Ferreira Alves , Leite Natália Gomes Ramalho , Barbosa Pedro Henrique Pontes , Sousa Daniela Heitzmann Amaral Valentim de , Arruda Isabela Tatiana Sales de , Souza Alysson Kennedy Pereira de 
Published: February 2025

A dislipidemia é um distúrbio metabólico que tem crescido bastante no Brasil, em consequência da urbanização, mudanças no padrão alimentar e no estilo de vida, tendo 18,4% da população brasileira diagnosticada com essa alteração. Tal distúrbio é caracterizado pelo aumento nos níveis de lipídios no sangue, envolve a elevação de colesterol e triglicerídeos no plasma e a redução dos níveis de HDL. Sendo assim, esse desequilíbrio eleva o risco de aterosclerose, que consiste no entupimento das artérias. Em pacientes tabagistas, adeptos ao cigarro tradicional ou eletrônico, essa relação é ainda mais acentuada, uma vez que, a nicotina presente no cigarro intensifica a probabilidade de problemas no sistema circulatório. O objetivo desse trabalho é compreender as consequências do tabagismo no desenvolvimento da dislipidemia no sistema circulatório. No que se refere a metodologia, trata-se de uma revisão sistemática da literatura com base nos registros na base de dados do LILACS, BDEFN. CUMED, CidSaúde, IBECS, MEDLINE, Sec. Est. Saúde Sp e Coleciona SUS. Desse modo, percebe-se que, ao inalar a fumaça do cigarro, seus compostos entram no sistema respiratório, alcançando a corrente sanguínea, por meio da circulação pulmonar, degradando artérias, vasos, tecidos e aumentando os riscos de acúmulo de lipídios. Nesse viés, as doenças que afetam o sistema circulatório são chamadas de doenças cardiovasculares (DCV), elas englobam as condições que impactam o coração ou os vasos sanguíneos e representam a principal causa de óbito e acometimento em nível global. Portanto, torna-se imprescindível discutir e conscientizar a população sobre os riscos associados ao tabagismo, bem como as suas consequências no sistema circulatório e na maior ocorrência de dislipidemias.