bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding healthcare professionals’ responses to patient complaints in secondary and tertiary care in the UK: A systematic review and behavioural analysis
Authors: Antonopoulou Vivi , Meyer Carly , Chadwick Paul , Gibson Beckie , Sniehotta Falko F. , Vlaev Ivo , Vasova Anna , Goffe Louis , Lorencatto Fabiana , McKinlay Alison , Chater Angel Marie 
Published: November 2023

Abstract

Background.
The path of a complaint and patient satisfaction with complaint resolution is often dependent on healthcare professionals’ (HCPs) first response. It is therefore important to understand the influences shaping HCP behaviour. This systematic review aimed to (1) identify the key actors, behaviours and factors influencing HCPs’ responses to complaints, and (2) apply behavioural science frameworks to classify these influences and provide recommendations for more effective complaints management.
Methods.
A systematic literature review of UK published and unpublished (“grey literature”) studies was conducted (PROSPERO registration: CRD42022301980). Five electronic databases (Scopus, Medline/Ovid, Embase, CINAHL, HMIC) were searched up to September 2021. Eligibility criteria included: studies reporting primary data, conducted in secondary and tertiary care, written in English and published between 2001–2021 (studies from primary care, mental health, forensic, paediatric, or dental care services were excluded). Extracted data included: participant quotations from qualitative studies, results from questionnaire and survey studies, case studies reported in commentaries, and descriptions and summaries of results from reports. Data were synthesised narratively using inductive thematic analysis, followed by deductive mapping to the Theoretical Domains Framework (TDF).
Results.
22 articles and 3 reports meeting the inclusion criteria were included. A total of 8 actors, 22 behaviours and 24 influences on behaviour were found. Key factors influencing effective management of complaints included HCPs’ beliefs about the value of complaints, knowledge of procedures and available time and resources, and organisational culture and leadership. Defensive practices and high stress levels among HCPs were linked to lack of managerial support, role conflict and a blaming culture within the organisation. Themes mapped predominantly onto the TDF domains of social influences (categorised both as barrier and enabler), beliefs about consequences (barrier) and social/professional role and identity (barrier). Recommendations were generated using the BCW approach.
Conclusions.
Through the application of behavioural science, we identified a wide range of individual, social/organisational and environmental influences on complaints management in secondary and tertiary care. Our behavioural analysis informed recommendations for intervention content, with particular emphasis on reframing and building on the positive aspects of complaints as an underutilised source of feedback at an individual and organisational level.
Preparation, structural characterization and enzymatic properties of alginate lyase immobilized on magnetic chitosan microspheres
Authors: Li Jinmeng , Yan Feng , Huang Bingbing , Zhang Mengyan , Wu Xiaodan , Liu Yuhuan , Ruan Roger , Zheng Hongli 
Published: November 2023

Abstract
Alginate lyase is an enzyme that catalyzes the hydrolysis of alginate into alginate oligoalginates. To enhance enzyme stability and recovery, a facile strategy for alginate lyase immobilization was developed. The novel magnetic chitosan microspheres were synthesized and used as a carrier to immobilize alginate lyase. Immobilization of alginate lyase on magnetic chitosan microspheres was successful as proved by the Fourier transform infrared spectroscopy and X-ray diffraction spectra. The enzyme immobilization exhibited best performance at MCM dosage = 1.5 g/L, adsorption time = 2.0 h, glutaraldehyde concentration = 0.2%, and immobilization time = 2.0 h. The optimal pH of the free alginate lyase was 7.5, and this pH value was shifted to 8.0 after immobilization. No difference was observed in the optimal temperature (45°C) for the immobilized and free enzymes. The immobilized alginate lyase displayed superior thermal stability compared with the free alginate lyase. The Km of the free and immobilized enzymes were 0.05 mol/L and 0.09 mol/L, respectively. The immobilized alginate lyase retained 72% of its original activity after 10 batch reactions. This strategy was a promising method to immobilize alginate lyase.
Extracellular CD30 and ADAM10/17 regulate brentuximab vedotin-induced cell death in an adult T-cell leukemia cell line
Authors: Sato Keisuke , Kozako Tomohiro , Nakano Akira , Ogata Kentaro , Kamimura Hidetoshi , Sasaki Hidenori , Takamatsu Yasushi , Takemoto Shigeki , Honda Shin-Ichiro 
Published: November 2023

Abstract

Adult T-cell leukemia/lymphoma (ATL) is a human T-cell leukemia virus 1 (HTLV-1)-induced malignancy of mature T lymphocytes with poor outcomes. Brentuximab vedotin (BV), which is an anti-CD30 antibody conjugated with monomethyl auristatin E, is included in the treatment of CD30-positive ATL, but there is no useful therapeutic marker for BV. Soluble CD30 (sCD30) in serum is increased in aggressive-type ATL at diagnosis, but the effects of extracellular CD30 on BV-induced cell death in ATL remain unclear. Similarly, a disintegrin and metalloproteinase (ADAM) 10 and 17 have CD30 sheddase activity in anaplastic large cell lymphoma, but this activity is unknown in ATL. The present study showed that sCD30 concentrations were related to BV activity in ATL-related cell lines. Extracellular vesicles (EVs) such as exosomes containing CD30 also inhibited BV activity. Additionally, the knockdown of ADAM10/17 significantly reduced sCD30 concentrations with increased cell death by BV. Our results suggest that ADAM10/17 are involved in sCD30 production in ATL. Moreover, endogenous extracellular CD30, such as sCD30 shed by ADAM10/17, and CD30-positive EVs may be responsible for the BV-induced cell death. Correctively, extracellular CD30 concentrations, including EV, may be useful as biomarkers for BV therapy in ATL.
Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
Authors: Kabbej Nadia , Ashby Frederick J. , Riva Alberto , Gamlin Paul D. , Mandel Ronald J. , Kunta Aishwarya , Rouse Courtney J. , Heldermon Coy D. 
Published: November 2023

Abstract

Background: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrogate species to humans.
Methods: A transcriptomic profile using RNA sequencing was generated for the temporal lobe, ventral midbrain, and cerebellum of 3 female and 3 male cynomolgus macaques previously treated with an Adeno-associated virus vector mix. Statistical analyses to determine differentially expressed protein-coding genes in all three lobes were conducted using DeSeq2 with a false discovery rate corrected P value of .05.
Results: We identified target genes in the temporal lobe, ventral midbrain, and cerebellum with functions in translation, immunity, behavior, and neurological disorders that exhibited statistically significant sexually divergent expression.
Conclusions: We provide potential mechanistic insights to the epidemiological differences observed between the sexes with regards to mental health and infectious diseases, such as COVID19. Our results provide pre-pubertal information on sexual differences in non-human primate brain transcriptomics and may provide insight to health disparities between the biological sexes in humans.
Analysis of clinical characteristics and risk factors of cervical intraepithelial neoplasia complicated with vaginal intraepithelial neoplasia
Authors: he sili , Jiang Jianfa 
Published: November 2023

Abstract
Objective: This study aims to explore the clinical characteristics and risk factors of cervical intraepithelial neoplasia (CIN) complicated with vaginal intraepithelial neoplasia (VaIN)
Methods: A total of 229 patients diagnosed with CIN complicated with VAIN, who received treatment at the Gynecology Department of the Third Xiangya Hospital of Central South University between January 2013 and January 2023, were included in this study. Additionally, 458 CIN patients treated during the same period were randomly selected as a control group. The clinical characteristics of both groups were compared and analyzed to identify risk factors for CIN complicated with VAIN.
Results: The mean age of patients in the CIN complicated with VAIN group was 49.84±14.03 years, with 55.5% being postmenopausal. In the CIN group, the mean age was 45.79±11.41 years, with 31.4% being postmenopausal. The difference between the two groups was statistically significant (p < 0.05). Among the CIN complicated with VAIN group, 97.4% of patients had HPV infection, with 46.7% being infected with multiple HPVs and 20.1% being infected with three or more HPVs. The infection rate was significantly higher than that in the CIN group (91.0%, 29.5%, 10.5%). There was a significant positive correlation between the grade of CIN and VAIN (Kappa=0.274, p < 0.05), indicating that as the grade of cervical lesions increased, so did the degree of vaginal lesions. Multifactor analysis revealed that menopause (OR=2.522), multiple HPV infections (OR=1.491), and three or more categories of HPV infections (OR=1.529) were independent risk factors for CIN complicated with VAIN (p < 0.05).
Conclusion: In order to provide early intervention and avoid missed diagnosis and treatment, it is important to pay attention to vaginal examination during colposcopy for postmenopausal and CIN patients with multiple HPV infections. This will help in timely detection of vaginal lesions.
Regulating sensitivity analysis of spatial patterns of high-density urban riverfront areas to the river cooling effect: a case study of the Grand Canal in Hangzhou, China
Authors: Zhang Xiaojian , Gong jiajia , Zhang jia 
Published: November 2023

Abstract
River blue-green spaces are an important type of urban cooling island, and their cooling effect is largely influenced by the spatial pattern of urban riverfront areas. These areas are the primary source of the urban heat island effect in high-density areas along urban rivers. However, the sensitivity of spatial pattern variables to the river cooling effect is not well understood. To address this question, the Grand Canal in the main city of Hangzhou, was selected as study area. This study identified three building space form variables and seven green space form variables based on area, spatial pattern, and correlated these indicators with land surface temperature using the Autogluon model, then calculated the river cooling effect from the land surface temperature, and tested the sensitivity of these spatial pattern indicators by comparing their effects on the river cooling effect. The results are that: (1) the Autogluon model accurately establishes the relationship between land surface temperature and spatial pattern indicators; (2) the river cooling index (RCI) of the Grand Canal in the main city of Hangzhou ranges from 0.67-5.35℃, with an average RCI of 2.62℃, and the river cooling distance (RCD) ranges from 25.6-370.4m, with an average RCD of 167.3m; (3) Building patch density, Overall greenspace patch cohesion index, and greenspace patch density exhibits high sensitivity to regulating the river cooling effect(RCE). This study provides valuable references for identifying key spatial pattern regulating indicators in urban riverfront spatial planning, and for enhancing the cooling effect of medium-sized urban cooling islands and optimizing the outdoor thermal comfort of areas along rivers through rapid simulation evaluation methods.
The Association of Leptin and Incident Hypertension in the REasons for Geographic and Racial Differences in Stroke (REGARDS) Cohort
Authors: Sherman Sarah , Stoutenburg Eric , Long Leann , Juraschek Stephen , Cushman Mary , Howard Virginia , Tracy Russell , Judd Suzanne , Mukaz Debora Kamin , Zakai Neil , Plante Timothy 
Published: November 2023

Abstract

Leptin is an adipokine associated with obesity and with hypertension in animal models. Whether leptin is associated with hypertension independent of obesity is unclear. Relative to White adults, Black adults have higher circulating leptin concentration. As such, leptin may mediate some of the excess burden of incident hypertension among Black adults. REGARDS enrolled 30 239 adults aged ≥ 45 years from 48 US states in 2003-07. Baseline leptin was measured in a sex- and race-stratified sample of 4 400 participants. Modified Poisson regression estimated relative risk (RR) of incident hypertension (new > = 140/>=90 mmHg threshold or use of antihypertensives) per SD of log-transformed leptin, stratified by obesity (BMI of 30 kg/m2). Inverse odds ratio weighting estimated the % mediation by leptin of the excess hypertension RR among Black relative to White participants. Among the 1 821 participants without prevalent hypertension, 35% developed incident hypertension. Obesity modified the relationship between leptin and incident hypertension (P-interaction 0.006) such that higher leptin was associated with greater hypertension risk in the crude model among those with BMI < 30 kg/m2, but not those with BMI ≥ 30 kg/m2. This was fully attenuated when adjusting for anthropometric measures. In the crude model, Black adults had a 52% greater risk of incident hypertension. Leptin did not significantly mediate this disparity. In this national U.S. sample, leptin was associated with incident hypertension among non-obese but not obese adults. Future investigations should focus on the effect of weight modification on incident hypertension among non-obese adults with elevated leptin.
Enhancing Analysis of EEG Image Data Sets: Dealing with Class Imbalances and Data Uncertainty
Authors: Mishra Sneha , Jaiswal Umesh Chandra 
Published: November 2023

Abstract
Quality Assurance and output focused algorithm design is the trend, which is a current day research challenge. Inter- and intra- behavioral changes in the system influence on quality of outputs, this due to class imbalance problem prevailing in the data sets, that read uncertain values causes class imbalance problem (CIP). Predictive models that depend on such states of data sets become highly imbalanced. As the CIP on uncertain data starts from selection of samples and candidates for the algorithms; the selected random samples project high variation of classes, a very smaller class or even larger than expected. EEG image data sets are generated from the electro-encephalogram are always random and often never repeating because they vary coarsely based on subjects, ailments and various levels of voltages generated at various types of montage points. Irrespective of montage points and the voltage generated from the electro-encephalogram, the outcome of the algorithms is to develop a predictive model of the subject’s epileptical state. In this regard, the problem of class imbalance (CIP) shall be addressed and handled with various types of heuristic and sampling methods. We shall work on various types of sampling methods and heuristics that develop a predictive model on the uncertain data and draw comparisons to select based on the uncertain state of the data.
Evaluating the Viability of Co-Firing Biomass Waste to Mitigate Coal Plant Emissions in Indonesia
Authors: Lou Jiehong , Squire Claire , Hilde Thomas 
Published: November 2023

Abstract

Reducing emissions in Indonesia’s energy sector requires mitigating coal plant emissions while avoiding land use change emissions. Given Indonesia’s abundant biomass resources, co-firing biomass presents an opportunity to reduce carbon emissions using agricultural residues, forestry waste, and municipal waste. We investigate whether available biomass waste is sufficient to meet feedstock requirements for co-firing at a range of ratios, whether demand can be met throughout the year and across the archipelago, and resulting emissions reductions. We conducted a feasibility study for each captive and non-captive coal plant in Indonesia, evaluating suitable biomass waste feedstocks including wood (both forest residues and industrial by-products), rubber, sugarcane, oil palm (kernel shell and empty fruit bunches), rice husk and straw, and municipal solid waste. Our results indicate that incorporating biomass waste into coal plants can reduce carbon emissions and contribute to the country's renewable energy targets without additional deforestation. However, while existing biomass waste is sufficient to meet co-firing demand at low ratios, competition with alternative uses and limited biomass supply in eastern provinces, where coal capacity is growing, preclude meeting demand at higher ratios. Our study provides insights into seasonal and spatial waste availability across Indonesia, and potential land use impacts and emissions reductions.
A prospective, multicenter, randomized controlled study comparing recyclable metal stents with endoscopic dilation in the treatment of benign anastomotic stricture after esophageal cancer surgery
Authors: Tao Zhang , Liu Jie , Pu Wenfeng , Ren Ninchuan , Feng Shenggang , Zhang Yan , Zhang Lisha , Li Feng , He Qingli , Lin Fengying , Du Zhonghan , Hu Xiaosan , Li Baohua , Zhang Guihua , Long Sizhe , Li Ying , Zhang Bing 
Published: November 2023

Abstract
Background
Endoscopic dilation (ED) is common and effective for improving strictures in patients with benign esophageal anastomotic stenosis (BEAS) after esophagectomy. A retrievable self-expanding metal stent has also been proven effective in treating strictures. However, evaluations comparing health economics of stent placement and ED for BEAS are lack.
Methods
In this prospective, randomized, controlled, multicenter study, BEAS patients between October 2020 and October 2021 at six centers were included. Patients were randomized into stent replacement (SR) or water balloon dilation (WBD) groups, and re-treatment interval times, body mass index (BMI) changes, dysphagia score, quality of life (QOL), treatment fees/times, total cost, daily cost, restenosis, and adverse effects assessed. Mann-Whitney U test was used for continuous variable analysis and Kaplan-Meier to calculate cumulative percentage and effective rate; significance was set at P < 0.05.
Results
Re-treatment interventions were more frequent in the WBD group (3 vs. 1.2, p < 0.05) and re-treatment interval times longer (60-days vs. 7-days, p < 0.05). Patient compliance was lower in the WBD group (p < 0.001), while treatment cost was higher in SR patients (5001 vs. 1812 yuan, p < 0.001). Daily treatment cost was lower in SR patients (78.57 vs 86.29 yuan, p < 0.05). QOL (97 vs. 86, p < 0.05), changes in posttreatment BMI (0 vs -0.72, p < 0.05), and posttreatment dysphagia score (1 vs. 2, p < 0.05) were higher in SR patients. There were three adverse events, two stent migrations, and one patient died following ED.
Conclusions
SR patients had higher QOL due to longer retreatment intervals, fewer endoscopic interventions, and lower daily treatment costs.
Trial registration
: The study was registered in the Chinese Clinical Trial Registry, number ChiCTR2000037864.
Endocytic cellular components determine secreta in female irradiated macrophages to promote intestinal regeneration
Authors: Lu Xingyu , Luo Guibing , Xu Yufan , Qiu Xinyu , Liu Yitong , Li Fang , Cui Fengmei , Chen Qiu 
Published: November 2023

Abstract
Background: The intestine is sensitive to radiation. After irradiation, the DNA of intestinal cells breaks and leaks, resulting in collagen deposition. Macrophages in the intestine perform cleaning and repair functions. However, the specific mechanism between cleaning and repairhas not yet been clarified. Methods and Results: Here, we found that after macrophages engulf DNA, the signal is transmitted to the CCAAT/enhancer binding protein beta (CEBPB) nuclear transcription factor through DEAD-box helicase 5 (DDX5) found by a coimmunoprecipitation assay. Next, a chromatinimmunoprecipitation assay showed that CEBPB bound to the promoter of the bone morphogenetic protein (BMP) inhibitory molecule Gremlin2 (GREM2) to increase GREM2 mRNA. Simultaneously, macrophages swallow collagen, and collagen inhibits HSP90AB1 (heat shock protein 90 kDa alphaB1) and CEBPB. Next, CEBPB suppresses the transforming growth factor (TGF) β inhibitory molecules latent transforming growth factor beta binding protein 1 (LTBP1) and decorin (DCN), leading to increased expression of LTBP1 and DCN in irradiated macrophages.
In the presence of estrogen and prolactin, the expression of GREM2, LTBP1, and DCN in irradiated macrophages significantly increased. GREM2 dose-dependently promoted crypt proliferation. Therefore, after depleting macrophages, the intestinal damage of female mice was significantly more severe than that of male mice after irradiation.
Conclusions: The data here showed that irradiated intestinal macrophages engulfed DNA and secreted GREM2 (positively regulated by CEBPB), while phagocytic collagen stimulated macrophages to secrete LTBP1 and DCN (negatively regulated by CEBPB). Estrogen will greatly amplify this mixed phenotype to promote intestinalrepair post ionizing radiation. These results suggest that there should be differences in the dosage of radiation therapy between male and female cancer patients.
Basal body contains a “latchbolt” to enable directional ciliary beat across epithelia
Authors: Zhu Xueliang , Pan Xinwen , Fang Chuyu , Shen Chuan , Li Xixia , Xie Lele , Li Luan , Huang Shan , Yan Xiumin 
Published: November 2023

Abstract

Motile cilia unify the orientation of their 9+2 axonemes across epithelia to drive directional liquid flows for various physiological functions. This planar polarity is believed to result from cytoskeleton-driven swiveling of the basal foot (BF), a basal body (BB) appendage that marks the axonemal orientation, in response to intrinsic and extrinsic cues. How and when the BF-axoneme relationship is established, however, are basic issues unaddressed. Here, we show that BFs and axonemal orientations are initially uncoupled and require a rotationally-asymmetric, Ccdc57-positive “latchbolt” in BB distal lumen to lock them in position. Furthermore, multicilia in individual mouse ependymal cells achieve uniform axonemal orientations and beat directions independently of BFs. Accordingly, Ccdc57-deficient multicilia display only cell-level directional beat but lack the planar polarity. This defect impairs cerebrospinal fluid flow, resulting in severe hydrocephalus, kyphosis, and high mortality in mice. These findings unravel brand-new mechanisms governing the planar polarity of epithelial motile cilia.