bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiparametric MRI combined with clinical factors to predict glypican-3 expression of hepatocellular carcinoma
Authors: Liu Peijun , Li Weiqiu , Qiu Ganbin , Chen Jincan , Liu Yonghui , Wen Zhongyan , Liang Mei , Zhao Yue 
Published: November 2023

ObjectivesThe present study aims at establishing a noninvasive and reliable model for the preoperative prediction of glypican 3 (GPC3)-positive hepatocellular carcinoma (HCC) based on multiparametric magnetic resonance imaging (MRI) and clinical indicators.MethodsAs a retrospective study, the subjects included 158 patients from two institutions with surgically-confirmed single HCC who underwent preoperative MRI between 2020 and 2022. The patients, 102 from institution I and 56 from institution II, were assigned to the training and the validation sets, respectively. The association of the clinic-radiological variables with the GPC3 expression was investigated through performing univariable and multivariable logistic regression (LR) analyses. The synthetic minority over-sampling technique (SMOTE) was used to balance the minority group (GPC3-negative HCCs) in the training set, and diagnostic performance was assessed by the area under the curve (AUC) and accuracy. Next, a prediction nomogram was developed and validated for patients with GPC3-positive HCC. The performance of the nomogram was evaluated through examining its calibration and clinical utility.ResultsBased on the results obtained from multivariable analyses, alpha-fetoprotein levels &gt; 20 ng/mL, 75th percentile ADC value &lt; 1.48 ×103 mm2/s and R2* value ≥ 38.6 sec-1 were found to be the significant independent predictors of GPC3-positive HCC. The SMOTE-LR model based on three features achieved the best predictive performance in the training (AUC, 0.909; accuracy, 83.7%) and validation sets (AUC, 0.829; accuracy, 82.1%) with a good calibration performance and clinical usefulness.ConclusionsThe nomogram combining multiparametric MRI and clinical indicators is found to have satisfactory predictive efficacy for preoperative prediction of GPC3-positive HCC. Accordingly, the proposed method can promote individualized risk stratification and further treatment decisions of HCC patients.
Comparison of the survival outcomes of laparoscopic, abdominal and gasless laparoscopic radical hysterectomy for early-stage cervical cancer: trial protocol of a multicenter randomized controlled trial (LAGCC trial)
Authors: Tang Xiaoyan , Zhou Shan , Zhang Xuyin , Hua Keqin , He Yuan , Wang Ping , Teng Yincheng , Feng Weiwei 
Published: November 2023

BackgroundRadical hysterectomy (RH) is considered a cornerstone in the treatment of early-stage cervical cancer. However, the debate surrounding the optimal surgical approach, whether minimally invasive or open surgery, remains controversial. The objective of this trial is to evaluate the survival outcomes of cervical cancer patients who undergo different surgical approaches.MethodsThis study is designed as a prospective, multicenter, open, parallel, and randomized controlled trial. A total of 500 patients diagnosed with stage IA1 with LVSI, IA2, IB1, or IB2 (2018 FIGO) will be recruited. Recruitment of participants started in November 2020. The participants will be randomly assigned to one of three groups: conventional laparoscopic RH, gasless laparoscopic RH, or abdominal RH. The primary endpoint of this trial is the 2-year disease-free survival (DFS) rate. The secondary endpoints will include the 2-year overall survival (OS) rate, 5-year DFS/OS, recurrence rates, operation time, intraoperative blood loss, surgery-related complications, and impact on quality of life (QoL).DiscussionWe expect this trial to provide compelling and high-quality evidence to guide the selection of the most appropriate surgical approach for early-stage cervical cancer.Clinical trial registrationChinese Clinical Trial Register, identifier ChiCTR2000035515.
Hyperconnectivity of the lateral amygdala in long-term methamphetamine abstainers negatively correlated with withdrawal duration
Authors: Li Yifan , Wang Xuhao , Huang Shucai , Huang Qiuping , Yang Ru , Liao Zhenjiang , Chen Xinxin , Lin Shuhong , Shi Yongyan , Wang Chenhan , Tang Ying , Hao Jingyue , Yang Jie , Shen Hongxian 
Published: November 2023

Introduction: Several studies have reported structural and functional abnormalities of the amygdala caused by methamphetamine addiction. However, it is unknown whether abnormalities in amygdala function persist in long-term methamphetamine abstainers.Methods: In this study, 38 long-term male methamphetamine abstainers (&gt;12 months) and 40 demographically matched male healthy controls (HCs) were recruited. Considering the heterogeneous nature of the amygdala structure and function, we chose 4 amygdala subregions (i.e., left lateral, left medial, right lateral, and right medial) as regions of interest (ROI) and compared the ROI-based resting-state functional connectivity (FC) at the whole-brain voxel-wise between the two groups. We explored the relationship between the detected abnormal connectivity, methamphetamine use factors, and the duration of withdrawal using correlation analyses. We also examined the effect of methamphetamine use factors, months of withdrawal, and sociodemographic data on detected abnormal connectivity through multiple linear regressions.Results: Compared with HCs, long-term methamphetamine abstainers showed significant hyperconnectivity between the left lateral amygdala and a continuous area extending to the left inferior/middle occipital gyrus and left middle/superior temporal gyrus. Abnormal connections negatively correlated with methamphetamine withdrawal time (r = −0.85, p &lt; 0.001). The linear regression model further demonstrated that the months of withdrawal could identify the abnormal connectivity (βadj = −0.86, 95%CI: −1.06 to −0.65, p &lt; 0.001).Discussion: The use of methamphetamine can impair the neural sensory system, including the visual and auditory systems, but this abnormal connectivity can gradually recover after prolonged withdrawal of methamphetamine. From a neuroimaging perspective, our results suggest that withdrawal is an effective treatment for methamphetamine.
Development of a UPLC-MS/MS method for the determination of lacosamide and its metabolite and its application to drug-drug interaction
Authors: Chen Jie , Shen Yuxin , Xia Hailun , Chen Xiaohai , Xu Ren-Ai , Lin Guanyang , Dai Gexin 
Published: November 2023

Lacosamide, a third-generation novel antiepileptic drug, was first approved in 2008 as an adjunct to partial seizures. In 2014, the U.S. Food and Drug Administration (FDA) approved it as a single agent for partial seizures. Since epilepsy is a chronic condition, most patients need long-term antiepileptic medicinal products, so it is even more important to consider the drug-drug interactions (DDIs). For the purpose of this experiment, an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay with accuracy and simplicity was optimized and fully validated for the simultaneous quantitative determination of lacosamide and O-Desmethyl-lacosamide (ODL), and DDIs between lacosamide and nisoldipine in vivo and in vitro was researched. The protein was precipitated with acetonitrile, the analytes were eluted with acetonitrile and a 0.1% formic acid solution in a gradient program, and lacosamide, ODL, and lamotrigine (Internal Standard, IS) were successfully separated by chromatography. The findings of the biological analysis revealed that the lower limit of quantification (LLOQ) for lacosamide in samples was 2 ng/mL and the linearity ranged from 2 to 10000 ng/mL. The LLOQ for ODL was 1 ng/mL, while the linearity range for this substance was 1–1,000 ng/mL. In rat liver microsomes (RLM), the LLOQ of ODL was 80 ng/mL and the linear range was 80–40000 ng/mL. The selectivity, stability, matrix effect and recovery rate were all satisfied with the need of quantitative analysis of samples. Then, the UPLC-MS/MS assay was employed successfully on the interactions of lacosamide and nisoldipine in vivo and in vitro. The half-maximal inhibitory concentration (IC50) was 3.412 μM in RLM, where nisoldipine inhibited the metabolism of lacosamide with a mixture of inhibition mechanism. In rat pharmacokinetic experiments, it was found that nisoldipine could significantly change the pharmacokinetic characteristics of lacosamide, including AUC(0-t), AUC(0-∞), Tmax, CLz/F and Cmax, but had no significant effect on ODL. In summary, the UPLC-MS/MS method could accurately and sensitively quantify lacosamide and ODL, and could be used for the interaction between nisoldipine and lacosamide in vivo and in vitro.
Prescription pattern and effectiveness of antihypertensive drugs in patients with aortic dissection who underwent surgery
Authors: Liao Kuang-Ming , Shen Chuan-Wei , Huang Yun-Hui , Lu Chun-Hui , Lai Hsuan-Lin , Chen Chung-Yu 
Published: November 2023

Background: Surgical patients with aortic dissection often require multiple antihypertensive drugs to control blood pressure. However, the prescription pattern and effectiveness of antihypertensive drugs for these patients are unclear. We aimed to investigate the prescription pattern and effectiveness of different classes of antihypertensive drugs in surgical patients with aortic dissection.Methods: Newly diagnosed aortic dissection patients who underwent surgery, aged &gt;20 years, from 1 January 2012 to 31 December 2017 were identified. Patients with missing data, in-hospital mortality, aortic aneurysms, or congenital connective tissue disorders, such as Marfan syndrome, were excluded. Prescription patterns of antihypertensive drugs were identified from medical records of outpatient visits within 90 days after discharge. Antihypertensive drugs were classified into four classes: 1) β-blockers, 2) calcium channel blockers (CCBs), 3) renin–angiotensin system, and 4) other antihypertensive drugs. Patients were classified according to the number of classes of antihypertensive drugs as follows: 1) class 0, no exposure to antihypertensive drugs; 2) class 1, antihypertensive drugs of the same class; 3) class 2, antihypertensive drugs of two classes; 4) class 3, antihypertensive drugs of three classes; or 5) class 4, antihypertensive drugs of four classes. The primary composite outcomes included rehospitalization associated with aortic dissection, death due to aortic dissection, and all-cause mortality.Results: Most patients were prescribed two (28.87%) or three classes (28.01%) of antihypertensive drugs. In class 1, β-blockers were most commonly used (8.79%), followed by CCBs (5.95%). In class 2, β-blockers+CCB (10.66%) and CCB+RAS (5.18%) were the most common drug combinations. In class 3, β-blockers + CCB+RAS (14.84%) was the most prescribed combination. Class 0 had a significantly higher hazard of the composite outcome (HR, 2.1; CI, 1.46–3.02; p &lt; 0.001) and all-cause mortality (HR, 2.34; CI, 1.56–3.51; p &lt; 0.001) than class 1. There were no significant differences in hazards for rehospitalization associated with aortic dissection among classes.Conclusion: Among operated patients with type A aortic dissection, no specific type of antihypertensive drug was associated with a better outcome, whereas among those with type B aortic dissection, the use of β-blockers and CCBs was related to a significantly lower risk of the composite outcome.
Overview of DISCOVER22 experiment in the framework of INFN-LNGS Cosmic Silence activity: challenges and improvements in underground radiobiology
Authors: Morciano Patrizia , Dini Valentina , Berardinelli Francesco , Baiocco Giorgio , Conte Valeria , Udroiu Ion , Barbato Federica , Marinaccio Jessica , Anello Pasqualino , Antoccia Antonio , Tabocchini Maria Antonella , Selva Anna , Canella Stefania , Bianchi Anna , Guardamagna Isabella , Lonati Leonardo , Scifoni Emanuele , Laubenstein Matthias , Balata Marco , Ferella Francesco , Grifoni Daniela , Galante Angelo , Maccarrone Mauro , Tirelli Valentina , Grasso Felicia , Sanchez Massimo , Sgura Antonella 
Published: November 2023

One of the most intriguing and still pending questions in radiobiology is to understand whether and how natural environmental background radiation has shaped Life over millions of years of evolution on Earth. Deep Underground Laboratories (DULs) represent the ideal below-background exposure facilities where to address such a question. Among the few worldwide DULs, INFN-Laboratorio Nazionale del Gran Sasso (LNGS) is one of the largest in terms of size and infrastructure. Designed and built to host neutrino and dark matter experiments, since the 1990 s the LNGS has been one of the first DULs to systematically host radiobiology experiments. Here we present the DISCOVER22 (DNA Damage and Immune System Cooperation in VEry low Radiation environment 2022) experiment recently started at LNGS. DISCOVER22 aims at investigating how the low radiation background modulates the Immune System (IS) response in in vitro and in vivo models. Underground radiobiology experiments are particularly complex and tricky to design and perform. In these studies, the accurate characterization of exposure scenarios is mandatory, but a challenging aspect is to understand how the very few ionizing tracks in the ultra-Low Radiation Environment (LRE) interact with the living matter in space and time in order to trigger different biological responses. In this Perspective, we describe these challenges and how we address them through a microdosimetric and a radiobiological approaches. We aim at linking physical microdosimetric measurements and the corresponding biological radiation responses by using radiation biophysical models that could shed light on many as yet unresolved questions.
Application of electrogastrogram in assessment of gastric motility in acute pancreatitis
Authors: Cai Ying , Wang Jinyun , Huang Deqiang , Luo Lingyu 
Published: November 2023

Background: Electrogastrogram (EGG) can reflect gastric motility disorders in many diseases, but its application in acute pancreatitis (AP) has not been studied. Therefore, our study aimed to investigate the value of EGG in assessing the existence of gastric motility disorder in patients with AP and in predicting the severity of AP.Methods: Patients with AP admitted to the First Affiliated Hospital of Nanchang University from June 2020 to December 2020 were enrolled. Five EGG parameters (Percentage of normal gastric slow wave (PNGSW), main frequency, average frequency, percentage of gastric tachycardia (PGT), percentage of gastric bradycardia (PGB)) were collected. The receiver operating characteristic (ROC) curve was constructed to judge the predictive value of EGG parameters to AP severity.Results: The PNGSW in AP patients was significantly lower than that of the control group (p &lt; 0.05), and the PGB was higher in AP patients than that of the control group (p &lt; 0.05). The area under curve (AUC) of the PNGSW and the PGB in diagnosing non-mild acute pancreatitis (N-MAP) were 0.777 (95% CI: 0.676-0.877, p &lt; 0.001) and 0.775 (95% CI: 0.670-0.879, p &lt; 0.001) respectively. After combining with C-reactive protein, the accuracy, sensitivity and specificity of predicting N-MAP were improved.Conclusion: EGG parameters can well reflect the gastric motility disorder of AP patients. The PNGSW and the PGB can be used to predict the occurrence of N-MAP.
Prevalence and spatial distribution of cranberry fruit rot pathogens in British Columbia, Canada and potential fungicides for fruit rot management
Authors: Wood Brandon , McBride Ethan , Nabetani Keiko , Griffin Taylor , Sabaratnam Siva 
Published: November 2023

Twenty-eight cranberry farms in southwestern British Columbia were investigated for the prevalence and spatial distribution of fungal pathogens that contribute to fruit rot incidence. Farms were selected from six regions where most cranberry production is concentrated. Flowers, and green and ripe fruit (var. ‘Stevens’) samples, collected during two consecutive crop seasons, were analyzed for fruit rot pathogens. The most frequently isolated pathogens were identified as Allantophomopsis cytisporea, Botrytis cinerea, Coleophoma empetri, Colletotrichum fioriniae, Colletotrichum gloeosporioides, Fusicoccum putrefaciens, Glomerella sp., Phomopsis vaccinii, Phyllosticta elongata, Phyllosticta vaccinii and Physalospora vaccinii. The pathogens Allantophomopsis cytisporea, Phyllosticta spp., and Physalospora vaccinii were found at high incidence. These pathogens were present in all cranberry growing regions, although their mean percentage incidence varied from farm-to-farm and region-to-region. Amongst the pathogens from three phenological stages of cranberry crop examined, ripe fruit had the highest percentage incidence of fruit rot pathogens compared to that of flowers or green fruit; thus, indicating their presence at the early stages of crop development. The efficacy to inhibit the mycelial growth and spore germination of the fruit rot pathogens by twenty six fungicides, belonging to nine different modes of actions, were evaluated in vitro. The copper-based fungicides and captan of group M, flutriafol, triforine, difenoconazole, prothioconazole and propiconazole of group 3, benzovindiflupyr of group 7, and fosetyl-Al of group 33 demonstrated a high degree of efficacy in inhibiting the mycelial growth of all fruit rot pathogens. The fungicides chlorothalonil of group M, fenbuconazole of group 3, pyrimethanil and cyprodinil of group 9, and fludioxonil of group 12 also demonstrated activity against most fruit rot pathogens. The copper-based fungicides, chlorothalonil, captan, flutriafol, triforine, difenoconazole, prothioconazole, propiconazole, benzovindiflupyr, and fosetyl-Al effectively prevented the spore germination of most fruit rot pathogens. This demonstrated activity of the fungicides towards cranberry fruit rot pathogens should be assessed for efficacy in planta under field conditions. The current study identified the most critical fungal pathogens causing fruit rot of cranberry in British Columbia and potential fungicides that could be used in the management of fruit rot and to improve fruit quality and yield.
Integrating the technology acceptance model for social media-based learning with authentic leadership development: symmetric and asymmetric modeling
Authors: Asghar Muhammad Zaheer , Rasool Samma Faiz , Seitamaa-Hakkarainen Pirita , Arif Seema , Bano Sumaira 
Published: November 2023

IntroductionThe growing trend of social media use has influenced all segments of society, including education, during the COVID-19 pandemic. At the same time, authentic leadership is an emerging concept in positive psychology for dealing with emergencies in the digital era. The possibility of a relationship between the two was checked in preservice teachers studying in a teacher education department of a university in Pakistan. The conceptual framework of the study was built around teaching acceptance model (TAM) and Authentic leadership theory.MethodsThe survey method suited the aim of the research, and questionnaires aided us in gathering participant self-reporting responses. We conducted two surveys, and in between them, a course was taught online using social media as a teaching and learning platform. The survey results checked preservice teachers’ positive intentions toward social media-based learning, and the post-course survey studied the development of Authentic leadership attributes among the preservice teachers. Technology acceptance was measured across three constructs: ease of use, usefulness, and positive intentions. The results also reported the development of three authentic leadership characteristics: ethics, self-regulation, and self-awareness.Results and discussionThis study is among the pioneering studies integrating TAM (i.e., the acceptance of social media-based learning) with leadership theory (i.e., authentic leadership). It also adds a methodological contribution by combining symmetrical (i.e., partial least squares structural equation modeling) and asymmetrical (i.e., fuzzy set qualitative comparative technique) for data analysis. The study’s findings are valuable for teacher education institutions, as they help prepare future teachers to become authentic leaders capable of addressing future crises by leveraging education through social media-based teaching and learning platforms.
Social networks and aggressive attitudes: who is who. Scoping review of the scientific production on their relationships
Authors: Martín-Martín Margarita , Bueno-Álvarez José Antonio 
Published: November 2023

IntroductionIn the current world, an increasing number of people use social networks as a scenario for socialization, which have come to stay as a part of human development. During this socialization process, violent situations occur all too often, despite their virtuality, and seriously compromises the emotional well-being of the other participants. Based on the work conducted on this subject, the following systematic review aims to establish the state of the art regarding the relationship between moral disengagement, disruptive behavior and emotional intelligence of social network users.MethodA scoping review is carried out, according to the PRISMA-ScR criteria, consulting the WoS, Scopus, Education database, PsycINFO, PsycARTICLES, PLOS one and ScienceDirect databases, from 2021 up to the present day.ResultsA total of 999 articles related to the research topic were collected, although the result of research responding to the specific search criteria was reduced to 10.DiscussionThe research identified shows that there is a relationship between the level of moral development of social network users and their participation in aggressive online behavior. However, more research is needed, as it has not been demonstrated whether it is the networks that develop or favor the emergence of these attitudes, or simply act as facilitators for their amplified expression.
Lower cortical thickness and increased brain aging in adults with cocaine use disorder
Authors: Schinz David , Schmitz-Koep Benita , Tahedl Marlene , Teckenberg Timo , Schultz Vivian , Schulz Julia , Zimmer Claus , Sorg Christian , Gaser Christian , Hedderich Dennis M. 
Published: November 2023

BackgroundCocaine use disorder (CUD) is a global health issue with severe behavioral and cognitive sequelae. While previous evidence suggests a variety of structural and age-related brain changes in CUD, the impact on both, cortical thickness and brain age measures remains unclear.MethodsDerived from a publicly available data set (SUDMEX_CONN), 74 CUD patients and 62 matched healthy controls underwent brain MRI and behavioral-clinical assessment. We determined cortical thickness by surface-based morphometry using CAT12 and Brain Age Gap Estimate (BrainAGE) via relevance vector regression. Associations between structural brain changes and behavioral-clinical variables of patients with CUD were investigated by correlation analyses.ResultsWe found significantly lower cortical thickness in bilateral prefrontal cortices, posterior cingulate cortices, and the temporoparietal junction and significantly increased BrainAGE in patients with CUD [mean (SD) = 1.97 (±3.53)] compared to healthy controls (p &lt; 0.001, Cohen’s d = 0.58). Increased BrainAGE was associated with longer cocaine abuse duration.ConclusionResults demonstrate structural brain abnormalities in CUD, particularly lower cortical thickness in association cortices and dose-dependent, increased brain age.