bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic relationship between the immune system and autism spectrum disorder and traits
Authors: Arenella Martina , Fanelli Giuseppe , Kiemeney Lambertus A. , McAlonan Grainne , Murphy Declan G. , Bralten Janita 
Published: October 2023

AbstractAutism spectrum disorder (ASD) is a common and complex neurodevelopmental condition. The pathophysiology of ASD is poorly defined; however, it includes a strong genetic component and there is increasing evidence to support a role of immune dysregulation. Nonetheless, it is unclear which immune phenotypes link to ASD through genetics. Hence, we investigated the genetic correlation between ASD and diverse classes of immune conditions and markers; and if these immune-related genetic factors link to specific autistic- like traits in the population.We estimated global and local genetic correlations between ASD (n=55,420) and 11 immune phenotypes (n=14,256–755,406) using genome-wide association study summary statistics. Subsequently, polygenic scores (PGS) for these immune phenotypes were calculated in a population-based sample (n=2,487) and associated to five autistic-like traits (i.e., attention to detail, childhood behaviour, imagination, rigidity, social skills), and a total autistic-like traits score. Sex-stratified PGS analyses were also performed.At the genome-wide level, ASD was positively correlated with allergic diseases (ALG), and negatively correlated with lymphocyte count, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) (FDR-p=0.01-0.02). At the local genetic level, ASD was correlated with RA, C-reactive protein, and granulocytes and lymphocyte counts (p=5.8x10-6–0.002). In the general population sample, increased genetic liability for SLE, RA, ALG, and lymphocyte levels, captured by PGS, was associated with the total autistic score and with rigidity and childhood behaviour (FDR-p=0.03).In conclusion, we demonstrated a genetic relationship between ASD and immunity that depends on the type of immune phenotype considered; some increase likelihood whereas others may potentially help build resilience. Also, this relationship may be restricted to specific genetic loci and link to specific autistic dimensions (e.g., rigidity).HighlightsAutism spectrum disorder (ASD) and diseases and traits linked to the immune system share genetic variabilityPositive genetic correlation is observed between ASD and atopic diseasesNegative genetic correlation is observed between ASD and autoimmune diseasesShared genetic loci between ASD and immune-related phenotypes influence gene expression in immune-related tissues and in the developing brainImmunogenetic factors associate with rigidity and childhood behaviour in the population
Genome-wide association study identifiesAPOEandZMIZ1variants as mitophagy modifiers in Lewy body disease
Authors: Hou Xu , Heckman Michael G. , Fiesel Fabienne C. , Koga Shunsuke , Soto-Beasley Alexandra I. , Watzlawik Jens O. , Zhao Jing , Valentino Rebecca R. , Johnson Patrick W. , White Launia J. , Quicksall Zachary S. , Reddy Joseph S. , Bras Jose , Guerreiro Rita , Zhao Na , Bu Guojun , Dickson Dennis W. , Ross Owen A. , Springer Wolfdieter 
Published: October 2023

AbstractThe PINK1-PRKN pathway mediates a critical quality control to maintain mitochondrial health and function. Together the kinase-ligase pair identifies and decorate damaged mitochondria with phosphorylated ubiquitin (p-S65-Ub). This selective label serves as the mitophagy tag and facilitates their degradation via autophagy-lysosome system. While complete loss of PINK1 or PRKN function causes early-onset Parkinson disease, much broader mitophagy impairments are emerging across neurodegenerative disorders. We previously found age- and disease-dependent accumulation of p-S65-Ub signal in the hippocampus of autopsy brains with Lewy body disease (LBD). However, the contribution of genetic variation to mitochondrial damage and p-S65-Ub levels remains unknown in LBD cases. To identify novel regulators of PINK1-PRKN mitophagy in LBD, we performed an unbiased genome-wide association study of hippocampal p-S65-Ub level with 1,012 autopsy confirmed LBD samples. Using an established, mostly automated workflow, hippocampal sections were immunostained for p-S65-Ub, scanned, and quantified with unbiased algorithms. Functional validation of the significant hit was performed in animal model and human induced pluripotent stem cells (hiPSCs).We identified a strong association with p-S65-Ub forAPOE4(rs429358;β: 0.50, 95% CI: 0.41 to 0.69;p=8.67x10-25) and a genome-wide significant association forZMIZ1(rs6480922;β: -0.33, 95% CI: -0.45 to -0.22;p=1.42x10-8). The increased p-S65-Ub levels inAPOE4-carrier may be mediated by both co-pathology-dependent and -independent mechanisms, which was confirmed in Apoe-targeted replacement mice and hiPSC-derived astrocytes. Intriguingly,ZMIZ1rs6480922 also significantly associated with increased brain weight and reduced neuropathological burden indicating a potential role as a resilience factor. Our findings nominate novel mitophagy regulators in LBD brain (ZMIZ1locus) and highlight a strong association ofAPOE4with mitophagy alteration. WithAPOE4being the strongest known risk factor for clinical Alzheimer’s disease and dementia with Lewy bodies, our findings suggest a common mechanistic link underscoring the importance of mitochondrial quality control.
Regulated Cleavage and Relocation of FERONIA Control Immunity in Arabidopsis Roots
Authors: Chen Jia , Qiang Xiaonan , Liu Hongbin , Xu Fan , Wang Long , Jiang Lingli , Li Chiyu , Wang Bingqian , Luan Sheng , Wu Dousheng , Zhou Feng , Yu Feng 
Published: October 2023

AbstractPlant roots exhibit localized immunity (LI) specifically in the root cap and transition/elongation zone (TZ/EZ). Such immunity relies on receptor-like kinases localized at the plasma membrane (PM), which are essential for plant response to bacteria in the rhizosphere. Here, we identified a new mechanism of action for receptor kinase FERONIA (FER) in LI. In the absence of bacterial stimuli, FER is localized to the PM, maintaining plant growth. When confronted with bacterial colonization, RALF23 peptide hyper-accumulates in TZ/EZ cells and activates a metalloproteinase that cleaves FER and transfer the cytosolic kinase domain into the nucleus. The nucleus-localized kinase domain of FER contributes to LI by reducing bacterial abundance at TZ/EZ. Thus, this work uncovered a unique mechanism by which a single receptor kinase acts in two molecular forms and two distinct subcellular locations to balance growth and immunity.One Sentence SummaryBacteria induces the cleavage of FER via At2-MMP to trigger a localized immunity around TZ and EZ of Arabidopsis roots.
The interplay between miRNAs and chromatin regulators underpins premature differentiation within neurodevelopmental disorders (NDDs)
Authors: Wood Jamie S , Alsaqati Mouhamed , Harwood Adrian J 
Published: October 2023

AbstractNeurodevelopmental disorders (NDDs), such as Schizophrenia, Autism and Intellectual disability (ID) possess a wide range of neurological and physiological phenotypes, often showing significant overlap. Epigenetic regulators, including miRNAs and chromatin modifiers, have long been proposed as a possible cause of the broad range of symptoms associated with NDDs. However, the inherent complexity within miRNA regulatory networks has made it challenging to identify the key miRNAs and their mRNA targets and how they lead to the molecular deficits seen in NDDs. Here we investigate a regulatory pathway that connects the epigenetic repressor EHMT1 to the miRNA network to control REST/NRSF protein expression and show that it controls the timing of neurodevelopment. By use of a novel strategy utilising multi-miR sponges we identify key miRNAs that regulate REST/NRSF in human induced pluripotent stem cells (iPSC) and demonstrate that they control unidirectional developmental progression from the pluripotent stage into early neurodevelopment and then neuronal differentiation. These results demonstrate a highly regulated interaction between epigenetic regulators and miRNAs that is crucial to control the timing neurodevelopment and impacts on the origins of NDDs.
Hundreds of antimicrobial peptides create a selective barrier for insect gut symbionts
Authors: Lachat Joy , Lextrait Gaëlle , Jouan Romain , Boukherissa Amira , Yokota Aya , Jang Seonghan , Ishigami Kota , Futahashi Ryo , Cossard Raynald , Naquin Delphine , Costache Vlad , Augusto Luis , Tissières Pierre , Biondi Emanuele G. , Alunni Benoît , Timchenko Tatiana , Ohbayashi Tsubasa , Kikuchi Yoshitomo , Mergaert Peter 
Published: October 2023

AbstractThe spatial organization of gut microbiota is crucial for the functioning of the gut ecosystem, although the mechanisms that organize gut bacterial communities in microhabitats are only partially understood. The gut of the insectRiptortus pedestrishas a characteristic microbiota biogeography with a multispecies community in the anterior midgut and a mono-specific bacterial population in the posterior midgut. We show that the posterior midgut region produces massively hundreds of specific antimicrobial peptides (AMPs), the Crypt-specific Cysteine-Rich peptides (CCRs) that have membrane-damaging antimicrobial activity against diverse bacteria but posterior midgut symbionts have elevated resistance. We determined by transposon-sequencing the genetic repertoire in the symbiontCaballeronia insecticolato manage CCR stress, identifying different independent pathways, including novel AMP-resistance pathways unrelated to known membrane homeostasis functions as well as cell envelope functions. Mutants in the corresponding genes have reduced capacity to colonize the posterior midgut, demonstrating that CCRs create a selective barrier and resistance is crucial in gut symbionts. Moreover, once established in the gut, the bacteria differentiate into a CCR-sensitive state, suggesting a second function of the CCR peptide arsenal in protecting the gut epithelia or mediating metabolic exchanges between the host and the gut symbionts. Our study highlights the evolution of an extreme diverse AMP family that contributes to establish and control the gut microbiota.
Physiological characterization of single gene lysis proteins
Authors: Antillon S. Francesca , Bernhardt Thomas G. , Chamakura Karthik , Young Ry 
Published: October 2023

AbstractUntil recently only 11 distinct Sgls (singlegene lysis proteins) have been experimentally identified. Of these, three have been shown to be specific inhibitors of different steps in the pathway that supplies Lipid II to the peptidoglycan (PG) biosynthesis machinery: Qβ A2inhibits MurA, ϕX174 E inhibits MraY, and Lys from coliphage M inhibits MurJ. These Sgls have been called “protein antibiotics” because the lytic event is a septal catastrophe indistinguishable from that caused by cell wall antibiotics. Here we propose to designate these as members of type I Sgls, to distinguish them from another Sgl, the L protein of the paradigm ssRNA phage MS2. Although none of the other distinct Sgls have significant sequence similarity to L, alignments suggested the presence of four domains distinguished by hydrophobic and polar character. The simplest notion is that these other Sgls have the same autolytic mechanism and, based on this, constitute type II.Although the number of experimentally confirmed Sgls has not changed, recent environmental metagenomes and metatranscriptomes have revealed thousands of new ssRNA phage genomes, each of which presumably has at least one Sgl gene. Here we report on methods to distinguish type I and type II Sgls. Using phase-contrast microscopy, we show that both classes of Sgls cause the formation of blebs prior to lysis, but the location of the blebs differs significantly. In addition, we show that L and other type II Sgls do not inhibit net synthesis of PG, as measured by incorporation of3[H]-diaminopimelic acid. Finally, we provide support for the unexpected finding by Adler and colleagues that the Sgl from Pseudomonas phage PP7 is a type I Sgl, as determined by the two methods. This shows that the sharing the putative 4-domain structure suggested for L is not a reliable discriminator for operational characterization of Sgls. Overall, this study establishes new ways to rapidly classify novel Sgls and thus may facilitate the identification of new cell envelope targets that will help generate new antibiotics.
Genetic transformation of the frog-killing chytrid fungusBatrachochytrium dendrobatidis
Authors: Kalinka Erik , Brody Stephanie M. , Swafford Andrew J. M. , Medina Edgar M. , Fritz-Laylin Lillian K. 
Published: October 2023

ABSTRACTBatrachochytrium dendrobatidis(Bd), a causative agent of chytridiomycosis, is decimating amphibian populations around the world.Bdbelongs to the chytrid lineage, a group of early-diverging fungi that are widely used to study fungal evolution. Like all chytrids,Bddevelops from a motile form into a sessile, growth form, a transition that involves drastic changes in its cytoskeletal architecture. Efforts to studyBdcell biology, development, and pathogenicity have been limited by the lack of genetic tools with which to test hypotheses about underlying molecular mechanisms. Here, we report the development of a transient genetic transformation system forBd. We used electroporation to deliver exogenous DNA intoBdcells and detected transgene expression for up to three generations under both heterologous and native promoters. We also adapted the transformation protocol for selection using an antibiotic resistance marker. Finally, we used this system to express fluorescent protein fusions and, as a proof of concept, expressed a genetically encoded probe for the actin cytoskeleton. Using live-cell imaging, we visualized the distribution and dynamics of polymerized actin at each stage of theBdlife cycle, as well as during key developmental transitions. This transformation system allows, for the first time, direct testing of key hypotheses regarding mechanisms ofBdpathogenesis. This technology also paves the way for answering fundamental questions of chytrid cell, developmental, and evolutionary biology.
Combating quiet quitting: implications for future research and practices for talent management
Authors: Liu-Lastres Bingjie , Karatepe Osman M. , Okumus Fevzi 
Published: October 2023

Purpose
This paper aims to offer viewpoints on the emergence of Quiet Quitting. Particularly, this paper reviews the reasons behind the phenomenon and analyzes its potential influences on the hospitality workforce. This study also proposes theory-driven solutions addressing this issue.

Design/methodology/approach
This paper is based on the relevant literature, industry reports and a critical reflection of the authors’ experiences, research and insights.

Findings
This paper reveals that Quiet Quitting can be a major obstacle for the hospitality business to reach service excellence. This paper also finds that Quiet Quitting is driven by several antecedents and correlates and affects employees, customers and various businesses in the hospitality and tourism industries.

Practical implications
This paper proposes several suggestions to properly address this issue, including enhancing the person–organization fit, work flexibility and employee well-being.

Originality/value
Quiet Quitting emerged as a new trend among the young workforce shortly after the pandemic. Despite the popularity of such odd terminology, academic discussions surrounding this issue have been limited. As one of the early attempts, this paper offers a critical analysis of the phenomenon and actional insights to respond to this ongoing challenge.
The impact of cryptocurrencies capitalization on banking deposits variability in the UAE: evidence from the NARDL approach
Authors: Adela Hatem Ahmed 
Published: October 2023

PurposeThis study aims to analyze the effect of cryptocurrency capitalization market development on bank deposits variability in the United Arab Emirates (UAE) spanning the period 2005M1–2020M4 using the novel nonlinear autoregressive distributive lag (NARDL).Design/methodology/approachThe study employs the NARDL recently developed by Shin et al. (2014) to estimate the long and short-run relationships between the variables rather than the widely known ARDL (Pesaran et al., 2001), which suffers from a complex structure in the estimation equation that usually includes lags and differences in both short and long terms. The implementation of NARDL required several proceedings after plotting the descriptive data, commencing with unit root tests, selection of lag length, estimating the long-and-short variables coefficients, heteroscedasticity test and Wald test for symmetries.FindingsThe long-run estimations of the positive and negative asymmetric coefficients indicate that cryptocurrencies capitalization has a negative impact on bank deposits in the UAE. Further, the short-run estimations coefficients exhibit that both significant positive and negative partial sum squares of cryptocurrencies decrease bank deposits.Research limitations/implicationsThe study has applied to the UAE spanning the period 2005M1–2020M4 using the NARDL.Practical implicationsThe short-run estimations coefficients exhibit that both significant positive and negative partial sum squares of cryptocurrencies decreases bank deposits, which means that the increase in the magnitude of cryptocurrencies capitalization stimulates depositors and speculators to adjust their portfolios towards contracting their deposits in banks to invest partially in cryptocurrencies, on the other hand, the decline in cryptocurrencies capitalization process spur depositors and speculators to reduce their deposits for purchasing cryptocurrencies at lower prices.Social implicationsThe study infers that individuals and businesses are cautious when investing in cryptocurrencies, and they need more certainty and trust to include these types of assets in their portfolios. The fluctuation in cryptocurrencies capitalization prompts speculators to change their deposits according to the cryptocurrencies' prices.Originality/valueThis study explores the short-and long-run asymmetric impacts of cryptocurrencies capitalization development on bank deposits volatility in the UAE, based on a NARDL, for providing a manifest depiction of whether the cryptocurrencies industry might be a threat to conventional banking system performance in the potential future.
Nigerian prisoners’ experience: prison circumstances, family support and sleep quality
Authors: Fakorede Omokehinde Olubunmi , Onifade Peter Olutunde , Majekodunmi Oluyinka Emmanuel , DadeMatthews Adefunke Omosefe 
Published: October 2023

Purpose
This study aims to determine the association between prison circumstances, perceived family support and sleep quality among male inmates at the Ibara prison, Ogun State.

Design/methodology/approach
It was a cross-sectional study of 300 male inmates interviewed with socio-demographic proforma and Pittsburgh sleep quality index.

Findings
While all respondents described the prison as being overcrowded, half of them used inappropriate sleeping materials daily and 41.4% decried a very noisy environment at night. Twenty-seven percent of the respondents described poor family support while 47.3% experienced poor sleep quality. Though nature of sleeping material and self-awareness of sleep problem were associated with poor sleep quality, no predictor of the latter was identified.

Research limitations/implications
The study was conducted among males only because of the relative dominance of the male gender in the prison population. This makes analysis of gender difference impossible. Furthermore, sleep studies may be preferred over reports by some sleep experts.

Practical implications
Findings from this study may culminate in taking steps to improve the Nigerian prison conditions as well as the prisoner’s welfare.

Social implications
Developing support group for relatives of inmates may be a focus of social intervention for these inmates. Also, inmates with good sleep quality are likely to partake effectively in the prison reformation programmes and may not self-medicate with sedatives.

Originality/value
This study provides a scholarly documentation of the state of Nigerian prisons; inmates’ perception of family support; prevalence and correlates of poor sleep quality among a group of male inmates.
The United Nations Sustainable Development Goals and human capital: the case of Azerbaijan
Authors: Sabbaghi Omid 
Published: October 2023

PurposeThis article aims to relate investments in human capital to the United Nations Sustainable Development Goals (UN SDGs), and examine the spending levels necessary to achieve high performance in related SDG sectors for Azerbaijan.Design/methodology/approachEmploying data from the World Bank, the empirical approach undertaken in this study relies on peer analysis by examining spending levels for nations exhibiting similar income levels and geographical proximity to Azerbaijan.FindingsThis study estimates that total spending in education would need to increase by 0.4 percentage points of GDP by 2030, while total spending in health would need to increase by 5.9 percentage points of GDP by 2030 for Azerbaijan.Originality/valueThis study contributes to the literature by conducting an empirical analysis in which other nations can emulate in measuring their relative progress on human capital investments and related UN SDGs.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-02-2023-0137
Exploring the antecedents of “deep” eWOM providing behaviour in mobile shopping: a multi-country study
Authors: Sahadev Sunil , Chung Sean , Zaman Mustafeed , Handoko Indria , Vo-Thanh Tan , Phong Nguyen Nguyen , Kumra Rajeev 
Published: October 2023

PurposeThe study aims to look at deep eWOM providing behaviour in m-commerce and attempts to explore its antecedents. Personalisation is proposed as an indirect antecedent of deep eWOM providing behaviour mediated by hedonic and utilitarian value perceptions and personal identification.Design/methodology/approachBased on social-exchange theory, the conceptual model links the study antecedents to deep eWOM providing behaviour. The conceptual model was validated through a multi-country study. A large sample of m-commerce users in the UK (n = 505), India (n = 422) and Vietnam (n = 618) were contacted to collect the data. Data were analysed through structural equations modelling procedure with invariance analysis conducted to ensure that the results from the three samples could be compared. The authors also conducted post-hoc analysis to explore the mediation paths between variables.FindingsThe study finds support to the conceptual model across the samples from the three countries. Personalisation is found to increase value perceptions – both utilitarian and hedonic – and personal identification which leads to “deep” eWOM providing behaviour across all the three countries. The serial mediation also provides comparable results across the three countries.Originality/valueThe study contributes to the understanding of deep eWOM providing behaviour – a construct with high practical relevance which has however not been explored sufficiently in current literature. The study also contributes to the literature that analyses the consequences of personalisation in m-commerce.