bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrasonographic contrast and therapeutic effects of hydrogen peroxide- responsive nanoparticles in a rat model with sciatic neuritis
Authors: Kim Da-Sol , Jo Nam-Gyu , Lee Dong-Won , Ko Myoung-Hwan , Seo Jeong-Hwan , Kim Gi-Wook 
Published: September 2023

Abstract
Background Peripheral nerve damage lacks an appropriate diagnosis consistent with the patient's symptoms, despite expensive magnetic resonance imaging or electrodiagnostic assessments that cause discomfort. Ultrasonography is valuable for diagnosing and treating nerve lesions; however, it is unsuitable for detecting small lesions. Poly(vanillin-oxalate) (PVO) nanoparticles are prepared from vanillin, a phytochemical with antioxidant and anti-inflammatory properties. Previously, PVO nanoparticles were cleaved by H2O2 to release vanillin, exert therapeutic efficacy, and generate CO2 to increase ultrasound contrast. However, the role of PVO nanoparticles in peripheral nerve lesion models is still unknown. Herein, we aimed to determine whether PVO nanoparticles can function as contrast and therapeutic agents for nerve lesions.
Methods To induce sciatic neuritis, rats were administered a perineural injection of carrageenan using a nerve stimulator under ultrasonographic guidance, and PVO nanoparticles were injected perineurally to evaluate ultrasonographic contrast and therapeutic effects. Reverse transcription-quantitative PCR was performed to detect mRNA levels of pro-inflammatory cytokines, i.e., tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2.
Results In the rat model of sciatic neuritis, PVO nanoparticles generated CO2 bubbles to increase ultrasonographic contrast, and a single perineural injection of PVO nanoparticles suppressed the expression of tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2, reduced the expression of F4/80, and increased the expression of GAP43.
Conclusions The results of the current study suggest that PVO nanoparticles could be developed as ultrasonographic contrast agents and therapeutic agents for nerve lesions.
SARS-CoV-2 remodels the landscape of small non-coding RNAs with infection time and symptom severity
Authors: Gomez Gustavo , Corell-Sierra Julia , Marquez-Molins Joan , Marqués María-Carmen , Montagud-Martínez Roser , Cebriá-Mendoza María , Cuevas José , Albert Eliseo , Navarro David , Rodrigo Guillermo 
Published: September 2023

Abstract
The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has significantly impacted global health, stressing the necessity of basic understanding of the host response to this viral infection. In this study, we investigated how SARS-CoV-2 remodels the landscape of small non-coding RNAs (sncRNA) from a large collection of nasopharyngeal swab samples taken at various time points from patients with distinct symptom severity. High-throughput RNA sequencing analysis revealed a global alteration of the sncRNA landscape, with abundance peaks related to species of 21-23 and 32-33 nucleotides. Principal component analysis successfully discriminated infected patients based on the sncRNA profiles. Host-derived sncRNAs, including microRNAs (miRNAs), transfer RNA-derived small RNAs (tsRNAs), and small nucleolar RNAs (sdRNAs) exhibited significant differential expression in infected patients compared to controls. Importantly, miRNA expression was predominantly down-regulated in response to SARS-CoV-2 infection, especially in patients with severe symptoms. Furthermore, we identified specific tsRNAs derived from Glu- and Gly-tRNAs as major altered elements upon infection, with 5’ tRNA halves being the most abundant species and suggesting their potential as biomarkers for viral presence and disease severity prediction. Additionally, down-regulation of C/D-box sdRNAs and altered expression of tyRNAs were observed in infected patients. These findings provide valuable insights into the host sncRNA response to SARS-CoV-2 infection and may contribute to the development of further diagnostic and therapeutic strategies in the clinic.
Transcriptomic and proteomic features of sperm DNA damage mouse model induced by benzo(a)pyrene
Authors: Zhang Chenming , Ma Yunfeng , Ma Sicheng , Zhang Qi , Chen Rubing , Hua Zhong , Wang Shiqi , Zhang Hao , Wang Zulong 
Published: September 2023

Abstract

BaP, also known as benzo(a)pyrene, is a prevalent organic contaminant that is primarily generated through the burning of fossil fuels. BaP has received widespread attention due to its high carcinogenicity and teratogenicity. The presence of BaP oxidative metabolites inside the human body has been indicated to be correlated with the occurrence of DNA damage in sperm and reduced sperm motility and concentration. The study replicated a mouse model of sperm DNA damage induced by BaP, and the transcriptome and proteome features of the model were examined. Male mice in both groups were subjected to BaP at a dosage of 100 mg/kg/d or an equivalent quantity of saline solution for a duration of 60 days. Subsequently, the assessment of the DNA fragmentation index (DFI) in sperm was carried out using a sperm chromatin structure assay (SCSA). To identify the expression patterns of mRNA and proteins in the testis, we used RNA-seq and data-independent acquisition (DIA). The BaP group had a significant elevation in sperm DFI. When comparing the control group to the BaP group, it was observed that the BaP group had differential expression of 240 genes (referred to as DEGs) and 616 proteins (referred to as DEPs). These included Aldh1a1, Cyb5r3, Fads1, Oxsm, Rcn3, and Prss45. Pathways in cancer, the PI3K-Akt signaling pathway, metabolic pathways, and the MAPK signaling pathway were the primary areas where these genes showed enrichment. Following a period of 60 days of intragastric delivery, our findings demonstrated that the male mouse model experienced sperm DNA damage due to the ingestion of BaP at a dosage of 100 mg/kg/d. The potential for BaP to cause harm to the reproductive system may be associated with its metabolic pathways and the PI3K-Akt signaling pathway.
Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile
Authors: Zhang Yiting , Cui Xiaowen , Lin Shuizhi , Lu Tao , Li Hao , Lu Yinghua , Cao Mingfeng , Lin Xihuang , Ling Xueping 
Published: September 2023

Abstract
Background:
The hydrolysis and transphosphatidylation of phospholipase D (PLD) play important roles in the interconversion of phospholipids (PLs), which has been shown to profoundly impact lipid metabolism in plants. In this study, the effect of the PLD1gene of Schizochytrium limacinum SR21 on lipid metabolismwas investigated.
Results:
PLD1knockout had little impact on cell growth and lipid production, but it significantly improved the percentage of polyunsaturated fatty acids in lipids, of which docosahexaenoic acid (DHA) content increased by 13.3% compared to the wild-type strain. Phospholipomics and qRT-PCR analysis revealed the knockout of PLD1reduced the interexchange and increasedde novo synthesis of PLs, which altered the composition of PLs, accompanied by a decrease in phosphatidylcholine (PC) and an increase in phosphatidylinositol, lysophosphatidylcholine, and phosphatidic acid levels. PLD1 knockout also increased DHA content in triglycerides (TAGs) and decreased it in PLs.
Conclusions:
These results indicate that PLD1 mainly performs the transphosphatidylation activity in Schizochytrium, and its knockout promotes the migration of DHA from PLs to TAGs, which is conducive to DHA accumulation and storage in TAGsvia an acyl CoA-independent pathway. This study provides a novel approach for identifying the mechanism of DHA accumulation and metabolic regulation strategies for DHA production in Schizochytrium.
Effect of dietary supplementation with lycopene on immune organ indices and immunological parameters of broilers fed a diet contaminated with AFB 1
Authors: Sarker Md Touhiduzzaman , Wan Xiao Li , Yang Hai Ming , Wang Zhi Yue , Zheng Chuntian , Chen Wei 
Published: September 2023

Abstract
Background: The present study was conducted to evaluate the effect of dietary lycopene (LYC) supplementation on immune organ indices, and immunological parameters of broilers fed a diet contaminated with aflatoxinB1 (AFB1). A total of 144 one-day-old Arbor Acres (AA) male broilers were purchased from a commercial hatchery (Nantong, Jiangsu Province, P.R. China). The broiler chicks were randomly allocated into 3 treatment groups with 6 replicates (cages) for each treatment and 8 broilers in each replicate. The three dietary treatments were as follows: (1) a basal diet (control); (2) a 100 µg/kg AFB1 contaminated diet; and (3) a diet supplemented with 200 mg/kg LYC and 100 µg/kg AFB1 (AFB1+LYC).
Results: The carcass characteristic results showed that the live body weight (BWT) was reduced (P < 0.05) in the AFB1-contaminated group compared to the control group. The supplementation of LYC significantly increased (P < 0.05) the BWT and the breast yield in comparison to the AFB1-contaminated group. On the other hand, the thymus weight was significantly decreased (P < 0.05) in the AFB1-contaminated group compared to the control group. Our results also demonstrated that 100 µg/kg AFB1 in the broiler diet could reduce the concentration of IgA (immunoglobulin A), CD3 and CD8 in the intestinal mucosa compared to those in the control group. The addition of dietary LYC increased the levels of IgA and CD3, and CD8 T-cell subsets in the broiler small intestine, implying that the immune function of the intestinal mucosa might be improved.
Conclusion: In conclusion, a broiler diet supplemented with LYC can alleviate live weight, immune organ weight, and increase immunological parameters by improving intestinal damage.
CDK inhibitors are a promising treatment for malignant melanoma with CIC mutation
Authors: Yu Biao , Zhang Xinwei , Yao Xinyuan , Qian Xiaoying , Wang Yong , Hong Weiwei , Fang Chen , Yuan Shangkun , Huang Cheng , Wang Tong , Zhou Bingbiao , Ye Xin , Li Yong 
Published: September 2023

Abstract
Background: Capicua (CIC) is a highly conserved high mobility group protein repressor that is mainly involved in mammalian development and homeostasis. Recent findings have shown that CIC mutation can lead to tumor progression and metastasis in various cancers, while the effect of CIC mutation on melanoma has not been reported. This study investigated the clinical and biological significance of CIC in melanoma.
Methods: This study reviewed the treatment of a 34-year-old Chinese female patient with malignant melanoma (MM) harboring CIC mutation. We further analyzed the mutational status, gene expression differences, and drug resistance of CIC from TCGA and GDSC databases. Simultaneously, we conducted A375, Hs294T cell proliferation, migration assays, Western blot assays for the downstream target gene expression after CIC knockdown, and in vivo animal experiments to analyze the effect of CIC expression on MM. Furthermore, CCK-8 and Clone-formation assays were conducted to assess the effect of the CDK inhibitor Dinaciclib on the proliferation of cells with decreased CIC expression.
Results: Here, we reported a metastatic MM harboring CIC mutation was effective with Lenvatinib combined with Crizotinib for the first time, while chemotherapy, cytokines, and immunotherapy were ineffective. In vitro and in vivo experiments demonstrated silencing CIC gene expression profoundly augmented cell proliferation and metastasis, while concurrently upregulating MMP-9 expression and stimulating EMT. Moreover, the CDK inhibitor Dinaciclib had a pronounced inhibitory effect on MM cells, regardless of CIC gene knockdown.
Conclusion: CIC gene knockdown boosts MM growth and metastasis and CDK inhibitors hold promise for treating malignant melanoma.
Physicochemical Properties and Molecular Characterization of Microbial Flora of Imported WheatFlour in Port Harcourt Metropolis
Authors: Ujong A.E , Ohanehi H.A 
Published: September 2023

Abstract
The Physicochemical properties and molecular characterization of microbial flora of imported wheat flour in Port Harcourt metropolis was investigated. Imported wheat flour was purchased from five locations in Port Harcourt. Flour samples were collected over a six weeks period on a weekly basis. The samples were subjected to physicochemical analysis, microbial counts and molecular identification of fungal species and compared with crushed wheat sample. The result of the physicochemical analysis over a six weeks period revealed a moisture range of 12–14%, protein content range of 10.95–13.21%, falling number of 260-387sec, particle size of 0.12–0.30µm and water absorption capacity range of 60.00-62.10%. Ash content of the flours collected ranged from 0.60–0.75% while that of the flour milled crushed sample ranged from 1.20–1.65% higher than the NIS standard. Throughout the collection period, moisture, ash, and protein contents of the wheat flour samples collected did not exceed standards set for wheat flour. Microbial counts of the samples also revealed total bacteria count range of NG (no growth) to 4.90×10− 4cfu/g, total mould count range of 1.50×10− 4-4.87×10− 4cfu/gand total yeast count range of NG to 4.49×10− 3cfu/g with low counts observed for the flour mill crushed wheat sample. Microbial counts of the samples throughout the period of collection were also within the acceptable statutory limits for wheat flour. Results of the molecular identification of the isolates shoed the presence of A.nigerand Aspergillus sydowii, Aspergillus niger, Fusarium lichenicola, Penicilliumgriseofulvum, Cladosporium tenuissimum and Hypopichiaburtonii. Of all the species isolates, Fusarium lichenicolawas the most frequently identified in the studied samples which encourages future studies on the research of toxins in these samples.It is therefore recommended that wheat flour for sales in open market by retailers should be stored in a cool and dry condition to prevent rapid spoilage and less performance.
Developing the theory of Toxic Chemotherapeutic Nutrition for Cancer Cells: Glucosodiene Polymer Structure, Safety, Efficacy, and Human Outcomes in Targeting Tumors via Glucose Mutation.
Authors: Akl Maher Monir , Ahmed Amr 
Published: September 2023

Abstract
Cancer is a complex genetic disease characterized by aberrant cellular behaviors, including uncontrolled growth, invasion, and metastasis. The development of personalized treatment strategies based on genomic profiling has led to improved outcomes. Recent scientific endeavors have focused on targeting cancer through metabolic approaches, capitalizing on the altered metabolic pathways in cancer cells. Glucosodiene polymer, a newly derived compound from glucose, has shown promising results in inhibiting glucose metabolism and modifying the tumor's microenvironment acidity. The Maher Akl Theory proposes a strategic approach to target cancerous tumors by inhibiting glucose metabolism and altering the tumor's microenvironment acidity using glucose isomer polymers. The goal is to disrupt the metabolic activity of the tumor and potentially modify and control the disease. This manuscript provides an overview of the metabolic vulnerabilities of cancer cells, evaluates the synthesis and chemical structure of glucosodiene, documents its safety, and explores its potential as a targeted therapy for cancer treatment. Additionally, a subset of successful clinical trials is presented, focusing on a case of successful treatment of triple-negative breast cancer (TNBC) with glucosodiene. The potential mechanisms of action of glucosodiene in cancer, including its impact on glucose metabolism, modulation of signaling pathways, and immune-enhancing effects, are discussed.
Integrated Paired Literacy Model Problem-Based Learning to Improve Biology Learning Outcomes
Authors: Faradila Anisa , Prafitasari Aulya Nanda , Farida Amaliyah 
Published: September 2023

The application of PBL learning can improve students' literacy skills because the learning activities require students to actively engage in literacy to find solutions to a given problem. Literacy skills are very important for students to have in learning biology; through these skills, students can improve their learning outcomes. Health literacy skills to support student learning outcomes are supported by the application of paired literacy techniques. Through the Classroom Action Research (CAR) method with a cyclical research model over 5 weeks, it is expected to be able to improve the learning outcomes of class XI Science. Data collection was carried out by testing techniques with quantitative data types. Therefore, it can be concluded that paired literacy activities in the PBL model can improve biology learning outcomes in the reproductive system material of class XI Science students with a classical completion percentage of 61.11%.
Inclusion of seismic refraction techniques in the characterization of the geological context for geothermal exploitation purposes
Authors: Sáez Blázquez Cristina , Martín Nieto Ignacio , Carrasco García Pedro , Farfán Martín Arturo , González-Aguilera Diego 
Published: September 2023

Given the growing importance of geothermal energy in the decarbonization of the energy sector, the application of appropriate methodologies that contribute to a greater knowledge of the resource is considered convenient. In this sense, refraction seismic techniques have proven to be a potential tool to predict the thermal behavior of the subsoil in locations where it is intended to implement a shallow geothermal system. From thermal conductivity measurements on samples with different degrees of compaction and consolidation, and from the propagation speeds of the P waves, a correlation pattern is developed that in turn allows obtaining 2D sections of the average thermal conductivity distribution in depth. The methodology developed shows that it is possible to estimate the evolution of the thermal conductivity parameter of the ground and thus guarantee an adequate design of the well field and the future correct operation of the geothermal system (providing the correct drilling length which would be underestimated by 30% with the standard thermal conductivity values).
Scoping the Priorities and Concerns of Parents: Infodemiology Study of Posts on Mumsnet and Reddit
Authors: Thornton Christopher , Lanyi Kate , Wilkins Georgina , Potter Rhiannon , Hunter Emily , Kolehmainen Niina , Pearson Fiona 
Published: September 2023

Background
Health technology innovation is increasingly supported by a bottom-up approach to priority setting, aiming to better reflect the concerns of its intended beneficiaries. Web-based forums provide parents with an outlet to share concerns, advice, and information related to parenting and the health and well-being of their children. They provide a rich source of data on parenting concerns and priorities that could inform future child health research and innovation.

Objective
The aim of the study is to identify common concerns expressed on 2 major web-based forums and cluster these to identify potential family health concern topics as indicative priority areas for future research and innovation.

Methods
We text-mined the r/Parenting subreddit (69,846 posts) and the parenting section of Mumsnet (99,848 posts) to create a large corpus of posts. A generative statistical model (latent Dirichlet allocation) was used to identify the most discussed topics in the corpus, and content analysis was applied to identify the parenting concerns found in a subset of posts.

Results
A model with 25 topics produced the highest coherence and a wide range of meaningful parenting concern topics. The most frequently expressed parenting concerns are related to their child’s sleep, self-care, eating (and food), behavior, childcare context, and the parental context including parental conflict. Topics directly associated with infants, such as potty training and bottle feeding, were more common on Mumsnet, while parental context and screen time were more common on r/Parenting.

Conclusions
Latent Dirichlet allocation topic modeling can be applied to gain a rapid, yet meaningful overview of parent concerns expressed on a large and diverse set of social media posts and used to complement traditional insight gathering methods. Parents framed their concerns in terms of children’s everyday health concerns, generating topics that overlap significantly with established family health concern topics. We provide evidence of the range of family health concerns found at these sources and hope this can be used to generate material for use alongside traditional insight gathering methods.
Economic Emigration from Poland to Latvia in 1928–1939
Authors: Grzybowski Jerzy 
Published: September 2023

The economic emigration from Poland to Latvia was a consequence of the socio-economic situation in both countries. The internal determinants of emigration were agrarian overpopulation and poverty in the Polish countryside. In this situation, many people saw a chance to improve their lives abroad. The problem of the overabundance of labourers in rural Poland coincided with a period of economic growth in Latvia. At a time when Poland was struggling with overpopulation and an excess of labour in the countryside, the Baltic country faced scarcity of hands in agriculture. The emigration movement to Latvia lasted from 1928 to 1939. The number of Polish workers employed in Latvian agriculture is estimated at over 100,000 people. The aim of the article is to analyse the origins, course and consequences of economic emigration from Poland to Latvia during this period.
Hacka Skolan. Ett arbete om: kunskaper och elevers blivande i skolan
Authors: Jason Norda  
Published: September 2023

Vad vill man ens lära sig?

Det här arbetet handlar om hur elever tillåtas undersöka sitt lärande i skolan. Hur man synliggör den kunskapen som elever har skaffat sig. Med ett processbaserat lärande, deleuziansk affektperspektiv och kunskapsteori ramas ett undersökande in där elevers vilja får styra och elever och lärare gemensamt lägger en grund för vilka kunskaper som ska belysas utan att lärarens förförståelse dikterar villkor. Genom ett undersökande i skolan tillsammans med nio gymnasieelever utvecklas också ett en egen fråga som rör vad ett forskande slutarbete kan vara, hur det kan gestaltas och läsas.

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What do you even want to learn?

This work is about how students are allowed to examine their learning in school. How to make the knowledge that students have acquired visible. With a process-based learning, Deleuzian affect perspective and epistemological theory, a framework is made for a research in which students' will is allowed to govern and where students and teacher can together lay a foundation for which knowledges can be understood without the teacher's pre-understanding dictating conditions. Through the research, which is made together with nine high school students, a separate question is also developed, asking what a final-project-research can be, how it can be written and read.

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Konstfack
Institutionen för bild- och slöjdpedagogik
Självständigt arbete i Bild 30 hp
Ämneslärarprogrammet med inriktning mot arbete i gymnasieskolan (Bild - Media)
VT22
Handledare: Miriam von Schantz & Miro Sazdic
Examinator: Anette Göthlund