bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocol for co-producing a framework and integrated resource platform for engaging patients in laboratory-based research
Authors: Lalu Manoj M. , Richards Dawn , Foster Madison , French Brittany , Crawley Angela M. , Fiest Kirsten M. , Hendrick Kathryn , Macala Kimberly F. , Mendelson Asher A. , Messner Pat , Nicholls Stuart G. , Presseau Justin , Séguin Cheryle A. , Sullivan Patrick , Thébaud Bernard , Fergusson Dean A. 
Published: February 2024

Abstract
Background
Patient engagement in research is the meaningful and collaborative interaction between patients and researchers throughout the research process. Patient engagement can help to ensure patient-oriented values and perspectives are incorporated into the development, conduct, and dissemination of research. While patient engagement is increasingly prevalent in clinical research, it remains relatively unrealized in preclinical laboratory research. This may reflect the nature of preclinical research, in which routine interactions or engagement with patients may be less common. Our team of patient partners and researchers has previously identified few published examples of patient engagement in preclinical laboratory research, as well as a paucity of guidance on this topic. Here we propose the development of a process framework to facilitate patient engagement in preclinical laboratory research.

Methods
Our team, inclusive of researchers and patient partners, will develop a comprehensive, empirically-derived, and stakeholder-informed process framework for ‘patient engagement in preclinical laboratory research.’ First, our team will create a ‘deliberative knowledge space’ to conduct semi-structured discussions that will inform a draft framework for preclinical patient engagement. Over the course of several sessions, we will identify actions, activities, barriers, and enablers (e.g. considerations and motivations for patient engagement in preclinical laboratory research, define roles of key players). The resulting draft process framework will be further populated with examples and refined through an international consensus-building Delphi survey with patients, researchers, and other collaborator organizations. We will then conduct pilot field tests to evaluate the framework with preclinical laboratory research groups paired with patient partners. These results will be used to create a refined framework enriched with real-world examples and considerations. All resources developed will be made available through an online repository.

Discussion
Our proposed process framework will provide guidance, best practices, and standardized procedures to promote patient engagement in preclinical laboratory research. Supporting and facilitating patient engagement in this setting presents an exciting new opportunity to help realize the important impact that patients can make.
Validation of an automated dispensing system for subsequent dose dispensing of different radionuclides
Authors: Cao T. T. , Aalbersberg E. A. , Geluk-Jonker M. M. , Hendrikx J. J. M. A. 
Published: February 2024

Abstract
Background
Automated dispensing systems (ADSs) for radiopharmaceuticals have been developed to reduce the radiation exposure of personnel, to improve the accuracy of the dispensed dose and to limit the microbiological contamination. However, before implementing such systems, validation according to various applicable guidelines is necessary to ensure safety and quality. Here we present the selection, validation and implementation of the PT459R2 from manufacturer Lynax s.r.o. as a guidance protocol for validation according to GMP and GRPP guidelines. Validation included linearity accuracy and precision of the internal scintillation detector for different isotopes and microbiological validation for aseptic procedures.

Results
The ADS can dispense accurate doses in the following linear range: 1000–10,000 MBq for lutetium-177, 20–74 MBq for zirconium-89, 100–1000 MBq for gallium-68 and 100–2000 MBq for fluorine-18. The maximum bias is 2.35% and the maximum coefficient of variation is 3.03% which meets the acceptance criteria of < 5%. Furthermore, the ADS does not affects the GMP class A environment in a laminar airflow cabinet and can dispense aseptically. In addition, radiation exposure is acceptable and data integrity is preserved.

Conclusion
The PT459R2 ADS met all the requirements from our performance qualification and is therefore suitable for daily routine use in our center. Our approach can be used as a guidance for PQ of an ADS in a Radiopharmacy according to GMP and GRPP guidelines.
The effect of early rehabilitation after lumbar spine surgery: a systematic review and meta-analysis
Authors: Özden Fatih , Koçyiğit Güldane Zehra 
Published: February 2024

Abstract
Background
Evidence-based data are required to provide insightful information on the timing of rehabilitation after lumbar spine surgery (LSS).

Objectives
The aim of this study is to systematically review the outcomes of early rehabilitation interventions and conduct its meta-analysis in patients after LSS.

Patients and methods
A total of 1183 articles were retrieved through PubMed (n = 793), Web of Science (n = 721), Scopus (n = 335), and ScienceDirect (n = 83) databases. Fourteen studies were included in the systematic review. The quality analysis and risk of bias assessment of the trials included in the systematic review were performed using the Physiotherapy Evidence Database (PEDro) scoring and classification system. Narrative synthesis and standardized mean difference based pooling results were given for the systematic review and meta-analysis, respectively.

Results
The additional benefit of early rehabilitation on physical function was moderately effective (ES: − 0.62, 95% CI − 1.00; − 0.25) at the 1-month follow-up. In terms of pain, early rehabilitation provided additional improvement at 1 month (ES: 0.34, 95% CI − 0.03; 0.71), 3 months (ES: − 0.14, 95% CI − 0.37; 0.10), 6 months (ES: 0.35, 95% CI 0.04; 0.65) and 1 year (ES: 0.21, 95% CI − 0.09; 0.52) follow-up at a low level of evidence.

Conclusions
This systematic review demonstrated that early rehabilitation mainly improved disability in the early period (1-month follow-up). Regarding pain, short-term (1 month) and mid-term (6 months) follow-ups showed the most significant additional benefit. The positive effects of starting rehabilitation early after surgery on pain may have positively affected disability, specifically in the early period (1 month).
Breast metastasis from primary hand rhabdomyosarcoma
Authors: Fares Nevien Ragae , Mohammed Tassnim Osama 
Published: February 2024

Abstract
Background
Rhabdomyosarcoma can occur almost anywhere in the body; however, the most common primary sites include the head and neck (50%), genitourinary tract (~ 25%), and extremities (~ 15%). Approximately 20% of rhabdomyosarcoma patients present with metastases at diagnosis, most commonly to the lungs, bone marrow, and lymph nodes. Rhabdomyosarcoma metastasizing to distant sites such as the breast highlights its aggressive behavior.

Case presentation
A 20-year-old girl presented with left-hand swelling and pain which was unfortunately excised without imaging evaluation. Postoperative histopathology revealed a malignant spindle cell tumor. After two months, the patient complained of rapidly growing bilateral breast masses. As regards the patient’s age diagnostic ultrasound was done that revealed bilateral breast masses with bilateral axillary lymphadenopathy (Fig. 1), and then, magnetic resonance imaging was done that revealed bilateral breast masses of malignant criteria and pathological left axillary lymph nodes (Fig. 2). An ultrasound-guided biopsy was taken from both breast masses which revealed a metastatic malignant spindle cell tumor. After immunophenotyping, the diagnosis of rhabdomyosarcoma is established. Unfortunately, a metastatic work-up was done that revealed a visceral and nodal metastasis as well as a myocardial deposit (Fig. 3).

Conclusions
Metastasis of rhabdomyosarcoma to the breast is rare and occurs in young women as in our case with mainly primary site in limbs. It is important to be clinically aware of the risk of breast metastasis in female patients with primary extra-mammary rhabdomyosarcoma.
Preparation and in vitro evaluation of BBG-250 loaded liposomal formulation for anticancer potential
Authors: Gupta Twinkle , Nema Priyanshu , Soni Sakshi , Yadav Vivek , Jain Sanyog , Soni Vandana , Kashaw Sushil K. 
Published: February 2024

Abstract
Background
Liposome-mediated drug delivery systems have emerged as a promising avenue for enhancing cancer treatment strategies. This study aims to develop and assess liposomal carriers loaded with Brilliant Blue G-250 (BBG-250), a potent P2X7 receptor antagonist that shows potential as an anti-tumor agent. Specifically, two types of liposomal formulations were designed: conventional liposomes composed of hydrogenated soya phosphatidylcholine (HSPC) and cholesterol, and pH-sensitive liposomes consisting of dioleoylphosphatidylethanolamine (DOPE), distearoylphosphatidylethanolamine-methoxy polyethylene glycol (DSPE-mPEG), dipalmitoylphosphatidylcholine (DPPC), and cholesterol. The investigation focuses on understanding the morphological characteristics, size, stability, drug incorporation efficiency, drug release profiles, blood compatibility, and cytotoxicity of these liposomal formulations.

Results
Advanced photon correlation spectroscopy using the Nano Plus-3 instrument was employed to evaluate the liposomes. The optimized conventional liposomes (HSPC-cholesterol ratio 7:3) exhibited a size of 125 ± 0.3 nm with a polydispersity index (PDI) of 0.21, indicating uniformity. The pH-sensitive liposomes (DOPE:DPPC:DSPE-PEG2000: Cholesterol 4:3:3:0.3) demonstrated a size of 118 ± 1.2 nm with a PDI of 0.230. Zeta potential measurements confirmed the stability of both formulations under physiological conditions, with values of − 16.93 mV for conventional liposomes and − 25.21 mV for pH-sensitive liposomes. Higher drug-to-lipid ratios were found to enhance drug incorporation efficiency. pH-sensitive liposomes exhibited superior drug release characteristics, with 95% release over 24 h, compared to conventional liposomes, which released 70% of the drug. Blood compatibility assessments revealed the safety of both formulations for intravenous administration. Cytotoxicity studies conducted on A549 cell lines demonstrated the cytocompatibility of both liposomal types across a range of concentrations, with IC50 values surpassing those of the reference drug, docetaxel.

Conclusions
This study underscores the potential of liposomal carriers as effective vehicles for delivering BBG-250, highlighting their stability, biocompatibility, and controlled drug release properties. Despite being slightly less potent than the reference drug, docetaxel, these liposomal formulations hold promise for advancing anticancer strategies. The findings contribute to the evolving landscape of innovative cancer therapy drug delivery systems, offering a novel approach to improving treatment outcomes for cancer patients. The successful development and evaluation of these liposomal carriers pave the way for further investigations and potential clinical applications in the field of cancer therapeutics.
Gastric foreign body granuloma resembling gastric cancer: a case report
Authors: Okasha Hussein Hassan , Alzamzamy Ahmed Elsayed , Delsa Hanane , Othman Haitham fekry , Alsibaie Ahmed sayed , Abdellatef Abeer 
Published: February 2024

AbstractFishbones are frequently swallowed by foreign objects that can penetrate the gastrointestinal tract. Nonetheless, it is quite uncommon for these fishbones to become lodged within the tract, resulting in the formation of foreign body granulomas that resemble submucosal tumors. When encountering this situation, it's important to consider differential diagnoses such as gastric intestinal stromal tumor, gastric leiomyoma, and gastric neurofibroma. We present a case of gastric foreign body granuloma that presents with dull aching epigastric pain. The patient gave a vague history of displaced intrauterine contraceptive devices (IUCD). Contrast CT and PET scan showed a gastric antral mass possibly a gastric cancer or a migrating IUCD. Endoscopic ultrasound (EUS) suggested a perigastric foreign body reaction due to the presence of a linear echogenic structure in between the markedly thickened gastric antral wall and the right lobe of the liver. EUS-guided fine needle biopsy (EUS-FNB) revealed non-specific inflammatory reaction. Afterward, surgical exploration unveiled the cause of the mass as a foreign body granuloma caused by a perforating fishbone.
Endobronchial chondrosarcoma presenting as recurrent pneumonia: a rare pulmonary tumour
Authors: Gupta Rahul Kumar , Mason Amita , Nadia Shirazi , Kumar Manoj 
Published: February 2024

Abstract
Background
Chondrosarcoma is a group of bone tumours made up of cells responsible for cartilage production. It usually affects the axial skeleton, including the pelvis, scapula and base of the skull with frequent metastasis to the lungs. Primary pulmonary involvement with chondrosarcoma is rarely seen.

Case presentation
In this case report, we will discuss a case of endobronchial chondrosarcoma presenting as recurrent pneumonia.

Conclusions
Only a few cases of primary pulmonary chondrosarcoma are reported with endobronchial involvement, therefore being quite rare.
An efficient image encryption algorithm using a discrete memory-based logistic map with deep neural network
Authors: Kumar B. Sakthi , Revathi R. 
Published: February 2024

AbstractIn the last few years, multimedia technology has made tremendous strides. These days, the Web is frequently used to transfer multimedia content, including audio, video, and photos. However, the Internet is a very vulnerable medium with many security holes. To ensure that multimedia content carried across unprotected channels, like the Internet, is secure and private, several encryption techniques have been proposed. New encryption strategies must be developed because multimedia data streams cannot be encrypted using traditional methods. Therefore, the main goal of the recommended system is to present an analytical research approach for introducing a sophisticated framework wherein the suggested encryption technologies' efficacy is increased through the use of deep neural networks (DNNs). The robustness of the DNN principle is coupled with a discrete memristor-based logistic chaotic map notion for enhanced security performance. In this paper, three distinct encryption algorithms—Arnie cat with an artificial neural network (ANN), Henon map with an ANN, and logistic map with a DNN—are compared for security and performance with the suggested algorithm. Correlation coefficients, information entropy, number of pixels changing rate (NPCR), encryption quality, and encryption duration are the cryptographic analysis parameters examined here. The results show that the recommended implementation enhances security performance without degrading image quality. The proposed algorithm achieves 35.9% of UACI, 99.95% of NPCR, and 7.997231 of entropy.
Channel estimation and MIMO combining architecture in millimeter wave cellular system with few ADC bits
Authors: Raji Akeem Abimbola , Orimolade Joseph Folorunso , Amusa Kamoli Akinwale , Adejumobi Isaiah Adediji 
Published: February 2024

AbstractHybrid combiner and precoder architectures, radio frequency (RF) chain, analog phase shifters, digital-to-analog converter (DAC), and analog-to-digital converter (ADC) are components of a millimeter wave cellular system. Prior works in the area of millimeter wave cellular system design employ receiver with infinite bit and large amount of RF chain that scales linearly with the quantity of transmitting and receiving antennas. This mode of design no doubt increases power demand or requirement of a typical millimeter wave system. In this work, hybrid architecture with few RF chains and small number of ADC bits are proposed and are used as candidate for millimeter wave channel estimation and cellular communication. In that connection, least square (LS), orthogonal matching pursuit (OMP), compressed sampling matching pursuit (CoSAMP), and deep learning (DL) techniques are utilized for analytical investigation. Indeed, computational results reveal that, when ADC consisting of uniform mid- rise quantizer is employed, the performance of 4 and 6 bits at signal-to-noise ratio (SNR) values of − 10 dB and 20 dB is at par with infinite bit (unquantized case). As a validation, DL compares favorably well with adaptive compressed sensing (ACS) technique previously used in the literature for channel estimation, while OMP and CoSAMP show better performance than ACS.
Alexithymia and peer victimisation: interconnected pathways to adolescent non-suicidal self-injury
Authors: Ruan Qian-Nan , Liu Linhui , Shen Guang-Hui , Wu Yu-Wei , Yan Wen-Jing 
Published: February 2024

Background
The prevalence of non-suicidal self-injury (NSSI) among adolescents underscores the importance of understanding the complex factors that drive this behaviour. Framed within broader constructs of emotional regulation theories, alexithymia and peer victimisation are thought to interact to influence NSSI behaviours.

Aim
This research addresses whether alexithymia and peer victimisation serve as risk factors for NSSI and, if so, how these factors interact with each other.

Method
This quantitative study analysed data from 605 adolescents, using a range of validated self-report measures including the Toronto Alexithymia Scale. Statistical analyses including one-way analysis of variance, multiple regression and structural equation modelling were employed to scrutinise the relationships among the variables.

Results
Alexithymia and peer victimisation significantly predicted NSSI behaviours. Specifically, the ‘difficulty in identifying feelings’ subscale of alexithymia emerged as a noteworthy predictor of NSSI (P < 0.001). Peer victimisation mediated the relationship between alexithymia and NSSI, explaining approximately 24.50% of alexithymia's total effect on NSSI. In addition, age was a significant predictor of NSSI, but gender and education years were not (P > 0.05). These relationships were found to be invariant across genders.

Conclusions
This study enriches our understanding of the interplay between alexithymia, peer victimisation and NSSI, particularly within the Chinese context. Its findings have significant implications for a rethinking of alexithymia's theoretical construct and interventions targeting emotional literacy and peer dynamics among adolescents. Future research could benefit from a longitudinal design to establish causality.
Effect of a GH Secretagogue, Anamorelin, on Serum Irisin and Inflammation Levels in Osteosarcopenic Adults
Authors: Dawson-Hughes Bess , Konieczynski Elsa M , Reitshamer Elise , Ceglia Lisa 
Published: February 2024

Abstract

Background
Data suggest an association between GH secretion and circulating levels of the myokine irisin and inflammatory cytokinesIL-6 and high-sensitivity C-reactive protein (hsCRP). The impact of GH secretagogues on these markers is unknown.

Objectives
To determine the effect of treatment with the GH secretagogue anamorelin on 12-month changes in serum irisin, IL-6, and hsCRP levels and to assess whether baseline irisin levels modulate the glycemic response to treatment with anamorelin.

Methods
This is an ancillary study in 26 older adults with osteosarcopenia who participated in a 12-month trial examining the effect of anamorelin 100 mg/day vs placebo on musculoskeletal outcomes. Serum irisin, IL-6, and hsCRP were measured at baseline and 12 months.

Results
Treatment with anamorelin, compared with placebo, did not significantly alter irisin levels [12-month change = 0.50 ± 1.2 (SD) ng/mL in anamorelin group and −0.08 ± 2.3 ng/mL in placebo; P = .191]. Baseline irisin levels were not significantly correlated with 2-month change in fasting glucose levels in the anamorelin group (r = −0.222, P = .46) or the placebo group (r = 0.30, P = .34); however, the slopes of the 2 regression lines describing the relationship by group tended to differ (P = .0547). Anamorelin treatment for 12 months had no significant effect on serum IL-6 or hsCRP levels.

Conclusion
In this small sample of older adults with osteosarcopenia, treatment with the GH secretagogue anamorelin did not significantly alter levels of irisin, IL-6, or hsCRP. Higher baseline irisin levels may attenuate the glycemic response to anamorelin treatment; however, a larger study is needed to confirm this possibility.
Automated counting of Drosophila imaginal disc cell nuclei
Authors: Sanchez Bosch Pablo , Axelrod Jeffrey D. 
Published: February 2024

ABSTRACT
Automated image quantification workflows have dramatically improved over the past decade, enriching image analysis and enhancing the ability to achieve statistical power. These analyses have proved especially useful for studies in organisms such as Drosophila melanogaster, where it is relatively simple to obtain high sample numbers for downstream analyses. However, the developing wing, an intensively utilized structure in developmental biology, has eluded efficient cell counting workflows due to its highly dense cellular population. Here, we present efficient automated cell counting workflows capable of quantifying cells in the developing wing. Our workflows can count the total number of cells or count cells in clones labeled with a fluorescent nuclear marker in imaginal discs. Moreover, by training a machine-learning algorithm we have developed a workflow capable of segmenting and counting twin-spot labeled nuclei, a challenging problem requiring distinguishing heterozygous and homozygous cells in a background of regionally varying intensity. Our workflows could potentially be applied to any tissue with high cellular density, as they are structure-agnostic, and only require a nuclear label to segment and count cells.
Recharging Retail: Estimating Consumer Demand Spillovers from Electric Vehicle Charging Stations
Authors: Babar Yash , Burtch Gordon 
Published: February 2024

Problem definition: We estimate the impact of electric vehicle (EV) charging stations on volumes of consumer foot traffic received by nearby retail establishments. We also explore the conditions under which any effects manifest. Methodology/results: We use a differences-in-differences design, exploiting the staggered introduction of Tesla Supercharger stations across the United States. We combine data on Supercharger installations with mobile phone–based estimates of retailer foot traffic. We explore heterogeneity in the treatment effect, in terms of EV charger characteristics, visitor characteristics, establishment type, and local physical context. We estimate that establishments experience an average 4% increase in monthly visits following the installation of a Tesla Supercharger. These effects arise primarily for retailers that offer relatively quick services (e.g., fast food) and for those located very near to the charger (within 150 meters). The effects are also more pronounced when the Supercharger is one of the first EV chargers introduced into the local area. Managerial implications: We document evidence of the positive retail demand spillovers arising from EV charging station infrastructure. We also document the conditions under which the benefits manifest. Insights for EV network operators, retailers, and policymakers are included. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2022.0519 .