bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of Leishmania RNA virus 2 (LRV2) on the virulence factors of L. major and pro-inflammatory biomarkers: an in vitro study on human monocyte cell line (THP-1)
Authors: Mirabedini Zahra , Mirjalali Hamed , Kazemirad Elham , Khamesipour Ali , Samimirad Katayoun , Koosha Mona , Saberi Reza , Rahimi Hanieh Mohammad , Mohebali Mehdi , Hajjaran Homa 
Published: December 2023

Abstract
Background
Cutaneous Leishmaniasis (CL) is a parasitic disease with diverse outcomes. Clinical diversity is influenced by various factors such as Leishmania species and host genetic background. The role of Leishmania RNA virus (LRV), as an endosymbiont, is suggested to not only affect the pathogenesis of Leishmania, but also impact host immune responses. This study aimed to investigate the influence of LRV2 on the expression of a number of virulence factors (VFs) of Leishmania and pro-inflammatory biomarkers.

Materials and methods
Sample were obtained from CL patients from Golestan province. Leishmania species were identified by PCR (LIN 4, 17), and the presence of LRV2 was checked using the semi-nested PCR (RdRp gene). Human monocyte cell line (THP-1) was treated with three isolates of L. major with LRV2 and one isolate of L. major without LRV2. The treatments with four isolates were administered for the time points: zero, 12, 24, 36, and 48 h after co-infection. The expression levels of Leishmania VFs genes including GP63, HSP83, and MPI, as well as pro-inflammatory biomarkers genes including NLRP3, IL18, and IL1β, were measured using quantitative real-time PCR.

Results
The expression of GP63, HSP83, and MPI revealed up-regulation in LRV2 + isolates compared to LRV2- isolates. The expression of the pro-inflammatory biomarkers including NLRP3, IL1β, and IL18 genes in LRV2- were higher than LRV2 + isolates.

Conclusion
This finding suggests that LRV2 + may have a probable effect on the Leishmania VFs and pro-inflammatory biomarkers in the human macrophage model.
Anomalous origin of left main coronary artery from the right sinus of Valsalva
Authors: Chua Frederick , Vongbunyong Kenny , Urgun Deniz Akay , Ghashghaei Roxana 
Published: December 2023

Abstract
Background
Anomalous coronary arteries are rare congenital variations with cases ranging from asymptomatic to life-threatening. Given the wide variability of coronary anomalies, it is challenging to predict their clinical consequences. Here, we present the ‘malignant’ variant – interarterial course of the left coronary artery between the aorta and pulmonary trunk – given the highest risk of sudden cardiac death among the various coronary anomalies.

Case presentation
Our case presents a 22-year-old male presenting to the emergency department after a syncopal episode that occurred while the patient was driving a motor vehicle. Initial Computed Tomography (CT) of the chest performed as part of the trauma work-up revealed a rare case of an anomalous origin of the left main coronary artery (LMCA) originating from a common ostium with the right coronary artery (RCA). The LMCA was found to have a malignant course, as it was positioned between the aorta and pulmonary artery. Given the high risk of sudden cardiac arrest with this congenital variant, the patient underwent coronary artery bypass grafting.

Conclusion
Anomalous coronary arteries remain the second leading cause of sudden cardiac death in young adult patients. The risk of sudden cardiac death depends on the congenital variant of the anomalous coronary artery as well as the course these vessels take. This case highlights a rare congenital variant featuring both the LMCA and RCA originating from a common ostium, with the LMCA having a malignant course, a variant with the highest risk of sudden cardiac death.
Trends and projections of inflammatory bowel disease at the global, regional and national levels, 1990–2050: a bayesian age-period-cohort modeling study
Authors: Zhou Jia-Li , Bao Jia-Chen , Liao Xu-Ying , Chen Yi-Jia , Wang Lin-Wei , Fan Yan-Yun , Xu Qin-Yu , Hao Lan-Xiang , Li Kun-Jian , Liang Ming-Xian , Hu Tian-Hui , Liu Zheng-Jin , Hu Yi-Qun 
Published: December 2023

Abstract
Background
Inflammatory bowel disease (IBD) is a global health concern with varying levels and trends across countries and regions. Understanding these differences is crucial for effective prevention and treatment strategies.

Methods
Using data from the 2019 Global Burden of Disease study, we examine IBD incidence, mortality, and disability-adjusted life years (DALYs) rates in 198 countries from 1990 to 2019. To assess changes in the burden of IBD, estimated annual percentage changes (EAPC) were calculated, and a Bayesian age-period-cohort model was used to predict the future 30-year trends of IBD.

Results
In 2019, there were 405,000 new IBD cases globally (95% uncertainty interval (UI) 361,000 to 457,000), with 41,000 deaths (95% UI 35,000 to 45,000) and 1.62million DALYs (95% UI 1.36–1.92million). The global age-standardized incidence rate in 2019 was 4.97 per 100,000 person-years (95% UI 4.43 to 5.59), with a mortality rate of 0.54 (95% UI 0.46 to 0.59) and DALYs rate of 20.15 (95% UI 16.86 to 23.71). From 1990 to 2019, EAPC values for incidence, mortality, and DALYs rates were − 0.60 (95% UI − 0.73 to − 0.48), − 0.69 (95% UI − 0.81 to − 0.57), and − 1.04 (95% UI − 1.06 to − 1.01), respectively. Overall, the burden of IBD has shown a slow decline in recent years. In SDI stratification, regions with higher initial SDI (high-income North America and Central Europe) witnessed decreasing incidence and mortality rates with increasing SDI, while regions with lower initial SDI (South Asia, Oceania, and Latin America) experienced a rapid rise in incidence but a decrease in mortality with increasing SDI. Predictions using a Bayesian model showed lower new cases and deaths from 2020 to 2050 than reference values, while the slope of the predicted incidence-time curve closely paralleled that of the 2019 data.

Conclusion
Increasing cases, deaths, and DALYs highlight the sustained burden of IBD on public health. Developed countries have stabilized or declining incidence rates but face high prevalence and societal burden. Emerging and developing countries experience rising incidence. Understanding these changes aids policymakers in effectively addressing IBD challenges in different regions and economic contexts.
Perceptions of HIV patients on the use of cell phones as a tool to improve their antiretroviral adherence in Northwest, Ethiopia: a cross-sectional study
Authors: Tizie Sefefe Birhanu , Shiferaw Atsede Mazengia , Beshir Miftah Abdella , Mengistie Muluken Belachew , Degualem Sayih Mehari , Assaye Bayou Tilahun 
Published: December 2023

Abstract
Background
Human immuno deficiency virus (HIV) is one of the most infectious diseases that cause death. A Medication non-adherence in HIV patient has been caused by factors such as not taking medications as prescribed by a physician, withdrawing from medication, missing appointments, and forgetfulness. To improve patients’ antiretroviral adherence, supporting them with mobile phone applications is advisable. This study aimed to assess HIV patients’ perceptions towards the use of cell phones to improve antiretroviral adherence.

Methods and materials
An institutional-based cross-sectional study was conducted among 423 HIV patients at a comprehensive specialized hospital in northwest Ethiopia from June to July 2022. Study participants were selected using systematic random sampling techniques and the data collection tool was adopted and modified for different literatures. Data were collected through an online data collection tool, and STATA-14 software was used for analysis. Descriptive statistics and binary logistic regression were used. The variables with a P-value equal to or less than 0.2 in bivariable logistic regression were entered into a multivariable logistic regression, and model fitness was assessed.

Results
A total of 410 study subjects have participated, making a response rate of 97%. In this study, 62% (95% CI: 57–67%) of HIV patients had a positive perception regarding the use of mobile phones to improve antiretroviral adherence. Perceived usefulness of mobile phones [AOR = 4.5, (95% CI: 2.2–9.1)], perceived ease of mobile phone use [AOR = 3.9, (95% CI: 2.0–7.5), age [AOR = 3.0, (95% CI: 1.5–6.2)], and educational status [AOR = 5.0, (95% CI: 2.3–10.0)] were significantly associated with HIV patients’ perception of mobile phones’ use to improve antiretroviral adherence.

Conclusions
More than half of the respondents had positive perception regarding the use of mobile phones to enhance their adherence to treatment. Perceived usefulness, perceived ease of use, age, and educational status was significantly associated with perception of mobile phone use to enhance antiretroviral therapy adherence. Therefore, the government have to encourage and support patients in incorporating mobile phones into their antiretroviral therapy (ART) follow-up through training.
Development of a patient-reported outcome measure of digital health literacy for chronic patients: results of a French international online Delphi study
Authors: Délétroz Carole , Del Grande Claudio , Amil Samira , Bodenmann Patrick , Gagnon Marie-Pierre , Sasseville Maxime 
Published: December 2023

AbstractBackgroundA psychometrically robust patient-reported outcome measure (PROM) to assess digital health literacy for chronic patients is needed in the context of digital health. We defined measurement constructs for a new PROM in previous studies using a systematic review, a qualitative description of constructs from patients, health professionals and an item pool identification process. This study aimed to evaluate the content validity of a digital health literacy PROM for chronic patients using an e-Delphi technique.MethodsAn international three-round online Delphi (e-Delphi) study was conducted among a francophone expert panel gathering academics, clinicians and patient partners. These experts rated the relevance, improvability, and self-ratability of each construct (n = 5) and items (n = 14) of the preliminary version of the PROM on a 5-point Likert scale. Consensus attainment was defined as strong if ≥ 70% panelists agree or strongly agree. A qualitative analysis of comments was carried out to describe personal coping strategies in healthcare expressed by the panel. Qualitative results were presented using a conceptually clustered matrix.ResultsThirty-four experts completed the study (with 10% attrition at the second round and 5% at the third round). The panel included mostly nurses working in clinical practice and academics from nursing science, medicine, public health background and patient partners. Five items were excluded, and one question was added during the consensus attainment process. Qualitative comments describing the panel view of coping strategies in healthcare were analysed. Results showed two important themes that underpin most of personal coping strategies related to using information and communications technologies: 1) questionable patient capacity to assess digital health literacy, 2) digital devices as a factor influencing patient and care.ConclusionConsensus was reached on the relevance, improvability, and self-ratability of 5 constructs and 11 items for a digital health literacy PROM. Evaluation of e-health programs requires validated measurement of digital health literacy including the empowerment construct. This new PROM appears as a relevant tool, but requires further validation.
Electricity generation and oxidoreductase potential during dye discoloration by laccase-producing Ganoderma gibbosum in fungal fuel cell
Authors: Umar Aisha , Abid Islem , Antar Mohammed , Dufossé Laurent , Hajji-Hedfi Lobna , Elshikh Mohamed S. , Shahawy Abeer El , Abdel-Azeem Ahmed M. 
Published: December 2023

AbstractColor chemicals contaminate pure water constantly discharged from different points and non-point sources. Physical and chemical techniques have certain limitations and complexities for bioenergy production, which motivated the search for a novel sustainable production approaches during dye wastewater treatment. The emerging environmental problem of dye decolorization has attracted scientist's attention to a new, cheap, and economical way to treat dye wastewater and power production via fungal fuel cells. Ganoderma gibbosum was fitted in the cathodic region with laccase secretion in the fuel cell. At the same time, dye water was placed in the anodic region to move electrons and produce power. This study treated wastewater using the oxidoreductase enzymes released extracellularly from Ganoderma gibbosum for dye Remazol Brilliant Blue R (RBBR) degradation via fungal-based fuel cell. The maximum power density of 14.18 mW/m2 and the maximum current density of 35 mA/m2 were shown by the concentration of 5 ppm during maximum laccase activity and decolorization of RBBR. The laccase catalysts have gained considerable attention because of eco-friendly and alternative easy handling approaches to chemical methods. Fungal Fuel Cells (FFCs) are efficiently used in dye treatment and electricity production. This article also highlighted the construction of fungal catalytic cells and the enzymatic performance of fungal species in energy production during dye water treatment.
Molecular pathways in the development of HPV-induced oropharyngeal cancer
Authors: Ullah Muhammad Ikram , Mikhailova Maria V. , Alkhathami Ali G. , Carbajal Nestor Cuba , Zuta Manuel Enrique Chenet , Rasulova Irodakhon , Najm Mazin A. A. , Abosoda Munther , Alsalamy Ali , Deorari Mahamedha 
Published: December 2023

AbstractOropharyngeal cancer, a subset of head and neck cancer, is increasingly recognized as a unique clinical entity primarily influenced by high-risk human papillomavirus (HPV) infections, particularly HPV-16. This review delves into the viral life cycle of HPV-16 and its interactions with host cells, with a specific focus on the crucial roles played by the viral oncoproteins E6 and E7. These oncoproteins drive cellular proliferation by targeting critical tumor suppressor proteins like p53 and Rb, resulting in uncontrolled cell growth and genomic instability. Furthermore, the significance of epigenetic modifications induced by HPV-16 and their implications is important for cancer progression. This comprehensive review provides valuable insights into the intricate molecular landscape of HPV-induced oropharyngeal cancer, shedding light on the development of targeted therapies and preventive strategies for this emerging global health concern.
Orthodontic treatment and biological limits: a retrospective clinical trial
Authors: Tabancis Niki Nikoleta , Krey Karl-Friedrich , Stahl Franka , Behnke Valeria , Ratzmann Anja 
Published: December 2023

Abstract
Background
The fundamental part of every successful orthodontic treatment is the detailed treatment planning including a precise determination of the virtual treatment objective (VTO) while considering the biological and anatomical limits. The aim of this study is to investigate and to compare the feasibility of the established reference values before and after orthodontic treatment and to determine the usefulness of this parameters as guidance for the sagittal anterior, sagittal posterior and transverse biological boundaries.

Materials and methods
Thirty-two patients aged 9 to 18 years (12 male and 20 female) with all permanent teeth present were randomly selected for orthodontic treatment with fixed multibracket appliance regardless of the potential malocclusion. The parameters 6-PTV, 1-NB [mm] and the WALA ridge were set for the identification of the transverse, sagittal anterior and sagittal posterior tooth position. The measurements were carried out at the beginning (T0) and at the end (T1) of the orthodontic treatment. They were set in relation with their individual threshold values (G). After the results of the measurements were conducted using the software OnyxCeph3TM (version 3.2.185 (505), Image Instruments GmbH, Chemnitz, DE), they were statistically calculated in the software RStudio (2022.12.0 Build 353 © 2009–2022 Posit Software PBC).

Results
Among the 32 patients, the mean pre- and post-treatment changes measured through the three parameters in relation to the individual reference values were statistically significant (p < 0.01). The mean values for 6-PTV, 1-NB and the WALA ridge amounted 15.37 mm, 2.56 mm and 4.23 mm at the beginning of the treatment, while after the treatment the measured values amounted 20.31 mm, 2.4 mm and 5.55 mm. These measurements combined with the statistical analysis of the changes of WALA ridge (T0, T1) confirmed that the teeth have been successfully uprighted and aligned. Furthermore, the maxillary first molars have been moved slightly mesially, as proven by the changes in 6-PTV, without certainty as to whether bodily movement or mesial tipping took place. Additionally, the lower incisors have been protruded, slightly exceeding the individual threshold values.

Conclusion
The parameters investigated provide a suitable assessment tool for recording the limits of the sagittal posterior, the sagittal anterior and the transverse dimension.
Ripening dynamics revisited: an automated method to track the development of asynchronous berries on time-lapse images
Authors: Daviet Benoit , Fournier Christian , Cabrera-Bosquet Llorenç , Simonneau Thierry , Cafier Maxence , Romieu Charles 
Published: December 2023

AbstractBackgroundGrapevine berries undergo asynchronous growth and ripening dynamics within the same bunch. Due to the lack of efficient methods to perform sequential non-destructive measurements on a representative number of individual berries, the genetic and environmental origins of this heterogeneity, remain nearly unknown. To address these limitations, we propose a method to track the growth and coloration kinetics of individual berries on time-lapse images of grapevine bunches.ResultsFirst, a deep-learning approach is used to detect berries with at least 50 ± 10% of visible contours, and infer the shape they would have in the absence of occlusions. Second, a tracking algorithm was developed to assign a common label to shapes representing the same berry along the time-series. Training and validation of the methods were performed on challenging image datasets acquired in a robotised high-throughput phenotyping platform. Berries were detected on various genotypes with a F1-score of 91.8%, and segmented with a mean absolute error of 4.1% on their area. Tracking allowed to label and retrieve the temporal identity of more than half of the segmented berries, with an accuracy of 98.1%. This method was used to extract individual growth and colour kinetics of various berries from the same bunch, allowing us to propose the first statistically relevant analysis of berry ripening kinetics, with a time resolution lower than one day.ConclusionsWe successfully developed a fully-automated open-source method to detect, segment and track overlapping berries in time-series of grapevine bunch images acquired in laboratory conditions. This makes it possible to quantify fine aspects of individual berry development, and to characterise the asynchrony within the bunch. The interest of such analysis was illustrated here for one cultivar, but the method has the potential to be applied in a high throughput phenotyping context. This opens the way for revisiting the genetic and environmental variations of the ripening dynamics. Such variations could be considered both from the point of view of fruit development and the phenological structure of the population, which would constitute a paradigm shift.
From data to action: a scoping review of wearable technologies and biomechanical assessments informing injury prevention strategies in sport
Authors: Rebelo André , Martinho Diogo V. , Valente-dos-Santos João , Coelho-e-Silva Manuel J. , Teixeira Diogo S. 
Published: December 2023

Abstract
Background
The purpose of this scoping review was to evaluate the current use of technologies in sports settings for training adaptation and injury prevention. The review aimed to map the existing literature, identify key concepts and themes, and highlight gaps in research, thus offering guidance for future studies.

Methods
This study followed the guidelines of the PRISMA extension for scoping reviews and a search in four major databases was conducted.

Results
A total of 21 studies were included. The findings highlighted the widespread use of various technologies, including wearable devices and force plates, to monitor athletes’ performance and inform evidence-based decision-making in training and injury prevention. Variables such as Player Load, changes of direction, and acute chronic workload ratio were identified as key metrics in injury prediction.

Conclusions
This review uncovers a dynamic field of research in athlete injury prevention, emphasizing the extensive use of varied technologies. A key finding is the pivotal role of Player Load data, which offers nuanced insights for customizing training loads according to sport-specific demands, player positions, and the physical requirements of various activities. Additionally, the review sheds light on the utility of tools like force plates in assessing fatigue, aiding recovery, and steering injury rehabilitation, particularly in sports prone to knee and ankle injuries. These insights not only enhance our understanding of injury prevention but also provide a strategic direction for future research, aiming to boost athlete safety, performance, and career longevity.
Translation, and validation of Dysphagia Outcome and Severity Scale (DOSS): Swedish version
Authors: Movander Klara , Larsson Palmquist Tove , Hägglund Patricia , Bergström Liza 
Published: December 2023

Abstract
Background
Swallowing dysfunction (dysphagia) significantly impacts patient and medical outcomes. In Sweden, there is no comprehensive outcome measure for dysphagia that incorporates holistic assessment and dysphagia impact on a person’s impairment, function and participation. The Dysphagia Outcome and Severity Scale (DOSS) was developed and validated (in English) and incorporates the World Health Organisation’s (WHO) aforementioned, International Classification of Functioning (ICF) aspects. This study translated then evaluated the validity and reliability of the Swedish version, DOSS-S.

Method
Translation occurred based on WHO recommendations. The Content Validity Index (CVI) of the translated version (DOSS-S) was assessed twice by 11 (multi-professional) dysphagia experts. Criterion validity and rater reliability was calculated using 18 Speech Pathologists assessing patient cases from International Dysphagia Diet Standardization Initiative—Functional Diet Scale (IDDS-FDS) research.

Results
Very high CVI values (0.96–0.99) for the linguistic correlation, and high CVI values (0.84–0.94) for applicability correlation were achieved. High criterion validity of DOSS-S with IDDSI-FDS was demonstrated (rs = 0.89, p < 0.01), with very high inter and intra rater reliabilities (ICC > 0.90).

Conclusion
The DOSS-S demonstrated very high validity values, and very high inter and intra rater reliability. This research contributes to improved dysphagia management by providing interprofessional dysphagia clinicians with a validated scale to identify patient progression, communicate dysphagia status between regions and countries, and document patient outcomes using an ICF framework.