bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine mapping and characterization of rust resistance genes Lr53 and Yr35 introgressed from Aegilops species
Authors: Xu Binyang , Shen Tao , Chen Hong , Li Hongna , Rehman Shams ur , Lyu Shikai , Hua Lei , Wang Guiping , Zhang Chaozhong , Li Kairong , Li Hao , Lan Caixia , Chen Guo-Yue , Hao Ming , Chen Shisheng 
Published: February 2024

Abstract

Wheat leaf rust and stripe rust are damaging fungal diseases of wheat worldwide. Breeding for resistance is a sustainable approach to control these two foliar diseases. In this study, we used SNP analysis, sequence comparisons and cytogenetic assays to determine that the chromosomal segment carrying Lr53 and Yr35 was originated from Ae. longissima or Ae. sharonensis or their derived species. In seedling tests, Lr53 conferred strong resistance against all five Chinese Pt races tested, and Yr35 showed effectiveness against Pst race CYR34 but susceptibility to race CYR32. Using a large population (3,892 recombinant gametes) derived from plants homozygous for the ph1b mutation obtained from the cross 98M71 × CSph1b, both Lr53 and Yr35 were successfully mapped to a 6.03 Mb telomeric region of chromosome arm 6BS in the Chinese Spring reference genome v1.1. Co-segregation between Lr53 and Yr35 was observed within this large mapping population. Within the candidate region, several nucleotide-binding leucine-rich repeat (NLR) genes and protein kinases were identified as candidate genes. Marker pku6B3127 was completely linked to both genes and accurately predicted the absence or presence of alien segment harboring Lr53 and Yr35 in 87 tetraploid and 149 hexaploid wheat genotypes tested. We developed a line with a smaller alien segment (< 6.03 Mb) to reduce any potential linkage drag and demonstrated that it conferred resistance levels similar to those of the original donor parent 98M71. The newly developed introgression line and closely linked PCR markers will accelerate the deployment of Lr53 and Yr35 in wheat breeding programs.
Predicting the Neurological Outcome in Survivors of Cardiopulmonary Resuscitation by Trans-cranial Doppler Measurment Values
Authors: soliman mohamed , Magdi Ahmed , Fatthy Moataz , Rostom Ahmed , Mostafa Rania , Azab Abdo Al 
Published: February 2024

Abstract

Background
Post-cardiac arrest brain injury is caused by insufficient cerebral blood flow which followed by reperfusion injury and manifested as global brain dysfunction. Neurologic outcome should be determined accurately by proving adequate restoration of the cerebral circulation and oxygen supply.
Aim of work:
Evaluate the Transcranial Doppler (TCD) measurements as a prognostic tool for neurological outcomes after cardiopulmonary resuscitation (CPR).
Methods
A prospective observational study includes 80 survivors of cardiac arrest with persistent coma after the return of spontaneous circulation (ROSC) admitted to the Critical Care medicine Department at Cairo University over 22 months. TCD parameters were measured at 24 and 72 h post-CPR and were analyzed compared to Cerebral Performance Category score (CPC).
Results
End diastolic velocity (EDV), Mean flow velocity (MFV) and pulsatility index (PI) measurements after 72 Hours of CPR showed significant correlation with neurologic outcome (P-value:<0.001, 0.021 and < 0.001, r: 0.79, 0.65 and 0.93 respectively). While, Peak systolic velocity (PSV) measurements after 24, 72 hours of CPR didn't show significant correlation with neurological outcome (P-value: 0.594 and 0.500 respectively). EDV, MFV & PI showed higher values in patients with favorable outcome in comparison to patients with bad outcome (21.84 vs. 13.46, 46.91 vs. 38.31 and 0.71 vs. 1.27).
Conclusion
TCD measurements can predict favorable neurological outcome after 72hrs of CPR via higher values of EDV, MFV and PI but this doesn’t apply earlier after 24 hrs of CPR.
Diurnal Variation in Anesthetic Recovery: Assessing Extubation Timelines Post Laparoscopic Appendectomy During Day or Night Inductions
Authors: Yang Zebin , Luo Ronghao , Liu Wenhai , Song Yali , Li Le 
Published: February 2024

Abstract

Background
The influence of diurnal variation on anesthetic drug metabolism and effectiveness is established, yet the impact on post-anesthetic extubation and intraoperative cardiac dynamics remains underexplored.
Objective
This investigation seeks to elucidate the impact of general anesthesia induction timing, specifically on extubation timelines and intraoperative vital sign fluctuations during laparoscopic appendectomies.
Methods
Employing a retrospective analysis approach, this study examines extubation timings in patients who underwent laparoscopic appendectomies over a three-year period (2020–2022). Grouping was done based on anesthesia induction timings: a day group (06:00–18:00) and a night group (18:00–06:00). The primary outcome assessed was the variance in extubation times between daytime and nighttime anesthesia inductions. Secondary measures included the frequency of intraoperative complications like hypertension, hypotension, and bradycardia.
Results
Data analysis from 576 laparoscopic appendectomy cases indicated a significant prolongation of extubation times in the night group compared to the day group. Notably, diurnal variations did not significantly affect the incidence of perioperative hypotension, hypertension, or bradycardia.
Conclusion
This study reveals a notable correlation between the diurnal variation and extubation timming in laparoscopic appendectomy patients, suggesting implications for nocturnal anesthesia management. These insights are instrumental in shaping optimal anesthesia protocols for emergency surgical procedures performed at night.
Effect of SARS-CoV-2 infection on human embryonic development and clinical outcomes: a retrospective cohort study
Authors: Tian Li , Sun Yiting , Jia Miaomiao 
Published: February 2024

Abstract
Objective
To investigate the effect of SARS-CoV-2 infection on embryonic development and clinical outcomes.
Methods
This retrospective analysis included 538 couples in December 2022. The couples were divided into two groups (COVID-19 group, n = 157; and non-COVID-19 [control] group, n = 381) according to whether one member of the couple had been infected with SARS-CoV-2 before oocyte retrieval. The general information, fertility rate, embryonic development and clinical outcomes were compared between the groups.
Results
There were no significant differences in baseline characteristics between the two groups. The rates of fertility, good-quality embryos and blastocyst formation were similar between the two groups. The separate effects of male or female infection on embryonic development were further analyzed. The IVF fertility rate was significant lower in the male COVID-19 group than in the control group (OR = 0.630, 95% CI = 0.510–0.776). In addition, the clinical pregnancy and live birth rate was significantly reduced in female patients who infected by SARS-CoV-2 compared to control group (OR = 0.018, 95% CI = 0.057–0.179).
Conclusion
This study shows that infection before oocyte retrieval does not have a clear negative effect on embryo outcomes, such as the rates of normal fertilization, good-quality embryos and blastocyst formation. However, infection before oocyte retrieval has negative effects on clinical outcomes in female patients.
The effect of Acacia eriolaba leaf meal inclusion on growth performance, blood parameters and methane gas emission in lambs fed diets containing ammoniated maize stover.
Authors: Chelopo Getrude Manakedi , Marume Upenyu 
Published: February 2024

Abstract

The study evaluated the effect of Acacia erioloba leaf meal in diets containing ammoniated maize stove on growth performance, methane emission and heath of growing lambs. Thirty-two female lambs were allocated to the following four dietary treatments: total mixed ration (TMR, control), 20% inclusion of untreated maize stover (UMS), 20% inclusion of ammoniated maize stover (AMS), and combined inclusion of 10% ammoniated maize stover and 10% Acacia erioloba leaves (AMSAL). Each treatment was replicated 8 times and a lamb in an individual pen was regarded as an experimental unit in a completely randomized design. Feed intake was higher (P < 0.05) in lambs fed the AMS and AMSAL diets compared to those fed UMS. Final body weights were higher in lambs fed the AMS and AMSAL diets. Both ADG and FCR were not affected (P > 0.05) by diet. In comparison with the AMS and AMSAL diets, the lambs fed the UMS diet had the highest (P < 0.05) Methane emission. Overall, lambs fed the control diets had the lowest methane gas emission. Hematological values were affected by diet with the AMSAL fed lambs having the highest (P < 0.05) MPV and PCT values. Furthermore, total albumin, amylase and total bilirubin were highest (P < 0.05) in lambs fed on the AMSAL diet. Lambs fed on AMS diet had the highest (P < 0.05) serum urea levels. It can be concluded that combined inclusion of ammoniated maize stover and Acacia leaves improved feed value and lamb performance when compared to the individual inclusion of both UMS and AMS.
A new and accurate approach to the Co2 haven hypothesis: The experience of the European Union and China
Authors: Avazdahandeh Somayeh 
Published: February 2024

Abstract

The pollution haven hypothesis (PHH) is defined as follows: A reduction in trade costs results in production of pollution-intensive goods shifting towards countries with easier environmental laws. The previous studies examined this hypothesis in the form of Kuznets' environmental hypothesis. In this way, they test the effect of foreign direct investment (FDI) on carbon emissions. However, this study investigates PHH from a new perspective. I will use Newton's gravity model to test this hypothesis. The basis of PHH is the difference in the environmental standards of the two business partners. One of the indicators used to measure the severity of a country's environmental laws is carbon emission intensity. The stricter the country's laws are, the lower the index value will be. In order to test the hypothesis, experimental data from China and OECD countries are used. China was as the pollution haven for the countries of the Organization for Economic Cooperation and Development. I found that environmental laws of host and guest countries have different effects on FDI. In addition, transportation costs have a negative effect on the FDI flow. Finally, the research results confirm the hypothesis on gravity model.
Factors associated with glycemic control in patients with T2DM: evidence from a cross-sectional study in China
Authors: Luo Wenting , Zhang Jiayu , Luo Yanxing , Wu Qiuwan , Chen Longfei , Liu Changqin , Lin Minqiang 
Published: February 2024

Abstract
ObjectiveThis study aimed to analyze the factors influencing glycemic control in patients with type 2 diabetes mellitus (T2DM).
MethodsBaseline data, encompassing basic information, lifestyle habits, and treatment of 305 T2DM patients from March 2021 to January 2023, were collected and analyzed using SPSS 26.0 software.
Results: Univariate and multivariate logistic regression analyses identified insulin therapy (OR=2.233, P=0.026) and regular clinic visits (OR=0.567, P=0.040) as independent factors influencing glycemic control. No observed interactions between the two variables were noted.
Conclusion: History of insulin therapy and regular clinic visits were significantly and independently associated with glycated hemoglobin control in T2DM patients. Tailored interventions based on individual circumstances are recommended to optimize glycemic control.
CXCL10-dependent epithelial-vascular cross-talk for endothelial activation following Sars-CoV-2 infection
Authors: Chaillot Laura , Blondot Marie-Lise , Recordon-Pinson Patricia , Pellegrin Isabelle , Boizard-Moracchini Andrea , Sliusar Myroslava , Pujol Nadège , Andreola Marie-Line , Bonnet Fabrice , Recher Gaelle , Andrique Leatitia , Nassoy Pierre , Mathivet Thomas , Bikfalvi Andreas 
Published: February 2024

Abstract
The vasculature is heavily impacted by SARS-CoV-2 infection. Conflicting results exist concerningthe mechanisms by which the SARS-CoV-2 virus acts on the vasculature. The presence of the virus within endothelial cells has been reported in patient samples. However, the ACE2 receptor wasnot detected in endothelial cells when analyzed by RNAseq analysis. Thus, how SARS-CoV-2 contributes to vascular inflammation and whether cross-talk between epithelial cells and endothelial cells is involvedare unclear. Therefore, we investigated the interaction between SARS-CoV-2 and the vasculature using 2D and 3D in vitro models, as well asour previously developed 3D vesseloid model. We first determined the suitability of the 3D vesseloid model for our study and then assessed whether SARS-CoV-2 is able to directly infect endothelial cells. In the absence of ACE2 in endothelial cells, no infection was detected. When ACE2 was overexpressed in endothelial cells, low uptake of viral particles by endothelial cells was observed without efficient viral production. We then explored the possibility that an indirect effect of SARS-CoV-2 infection involvesepithelial-endothelial cellcross-talk. After infection of the epithelial cells, a significant inflammatory response was detected in the endothelial cells. Furthermore, we investigated the cytokines possibly implicated and identified CXCL10 as the most highly expressed proinflammatorycytokine and explored its function in this context. Finally, the clinical relevance of our findings was confirmed by evaluating CXCL10 and alternative cytokine dosages in blood samples fromSARS-CoV-2-infected patients, which were validated in silico in an independent patient cohort.
Additive Manufacturing Based On Pulsed GMAW With Stainless Steel 308L - A Method for Parameter Definition and Characterization of Metallic Transfer Modes
Authors: Ribeiro Túlio José Bernardes , Coelho Fagner Guilherme Ferreira , Arias Ariel Rodríguez , Rezende Rogério Ferreira 
Published: February 2024

Abstract
The use of the GMAW-P process shows promise in expanding the possibilities of metal additive manufacturing due to its high deposition rates, lower material costs compared to other processes, and due to its ability to provide better control over heat input and melt pool during operation. Since the droplet detachment mode is an important factor for process stability, further investigation is justified regarding the impact of the different modes on the formation of deposited material layers. In order to facilitate parameter selection for this and other research related to droplet detachment conditions with stainless steel 308L, it was proposed to map the regions of One Drop per Pulse, Multiple Drops Per Pulse, and One Drop per Multiple Pulses. Furthermore, the aim is to validate the use of the detachment constant as a means to estimate the detachment condition for a specific set of peak current and peak time. To perform this mapping, a high-speed camera was used to visually validate the droplet detachment modes obtained by varying the peak time and peak current. The results allowed the determining intervals for each of the three droplet detachment modes. It was also observed that the detachment constant can be a good approximation for the phenomenon, provided the same wire, contact-tip-to-work distance, shielding gas, and mean current are used.
Mental health outcomes and socio-demographics as determinants of students' academic performance and smartphone addiction at the University of Cape Coast
Authors: Ninnoni Jerry Paul , Ijere Ignatius , Iloma David O. , Armah-Mensah Isaac , Commey Isaac Tetteh , Agyemang Sampson Opoku , Nsatimba Frederick , Attipoe Elliot Kojo 
Published: February 2024

Abstract
Objectives
Smartphone addiction is a significant mental health concern that affects students’ academic performance, with attendant consequences on their psychological well-being, and yet only a handful of empirical research has been conducted on this area in Sub-Saharan Africa. The study, therefore, assessed mental health outcomes and socio-demographics as determinants of students' academic performance and smartphone addiction among students.
Methods
Employing a descriptive and analytical cross-sectional study design, purposive sampling was used to recruit 227 School of Nursing and Midwifery students. The study variables were measured with the Depression Anxiety Stress Scale (DASS-21) and Smartphone Addiction Scale.
Results
The results showed that 89 (39.2%) male and 138 (60.8%) female nursing students participated in the study. Employing descriptive statistics, we found a 43.6% prevalence of smartphone addiction. Pearson correlations showed significant associations between mental health outcomes and smartphone addiction. Utilising multi-linear regressions, socio-demographic factors and mental health outcomes did not jointly predict smartphone addiction, whereas mental health outcomes independently predicted smartphone addiction. Hierarchical regressions revealed that student’s socio-demographics and mental health outcomes significantly influenced their academic performance, particularly for variables such as ‘gender’, ‘age’, ‘level of study’ and ‘mental health outcomes’. Multivariate Analysis of Variance (MANOVA) revealed that age, level of study, marital status and mental health outcomes reported main and interaction effects on academic performance and smartphone addiction.
Conclusion
The study observed that smartphone addiction is remarkably high among students and should be addressed, while concerted efforts should be made to address depression, anxiety and stress among students, which pushes them into addictive behaviours. Recommendations for clinicians, educationists, social media experts and information technology professionals were drawn for positive coping, adjustment, and well-being.
Effects of Immunotherapy on Quality-of-Life Outcomes in Patients with Gastroesophageal Cancers: A Meta-Analysis of Randomized Controlled Trials
Authors: Gupta Kush , Roy Arya Mariam , Attwood Kristopher , Nipp Ryan David , Mukherjee Sarbajit 
Published: February 2024

Abstract

Background
Immune checkpoint inhibitors (ICIs) have revolutionized cancer care, with increasing data demonstrating improved survival outcomes using ICIs among patients with advanced gastroesophageal cancer (GEC). ICIs are also associated with a lower incidence of grade ≥ 3 adverse events (AEs) compared to chemotherapy, suggesting that ICIs may have favorable effects on health-related quality of life (HRQoL). This meta-analysis sought to evaluate the effects of ICIs on the HRQoL of patients with advanced GEC.
Methods
We conducted an online bibliographic search on Medline via PubMed using MeSH-based terms to retrieve randomized controlled trials (RCTs) that evaluated the effects of ICIs on HRQoL in patients with advanced GEC (we searched for all studies between 2018–2021). We included RCTs that incorporated ICIs as part of the intervention arm either as monotherapy (first- or second-line) or as a combination therapy (first-line) with another ICI or chemotherapy. We combined the HRQoL measures into a meta-analysis using standard random effects models, from which estimates of the average mean difference (MD) were obtained with 95% confidence intervals. We assessed heterogeneity of the study outcomes using the Q and I2 statistics.
Results
We identified 11 phase-3 RCTs that met inclusion criteria, with a mean enrollment of 820 patients. Eight RCTs had ICI plus chemotherapy combination in the intervention arm, three had ICIs as monotherapy, and one had doublet ICI therapy in the intervention arm. All RCTs had chemotherapy for the control arm. Collectively, trials reported 37 HRQoL measures using 5 different HRQoL tools. The pooled analysis favored the intervention over the control arm in terms of Functional Assessment of Cancer Therapy-Esophageal (FACT-E) scores [MD 2.7 (95% CI 0.1 to 5.3), p < 0.041]. In a subgroup analysis of eight RCTs comparing combination therapy with ICIs plus chemotherapy versus chemotherapy alone, the effect estimates favored the ICI arm regarding the FACT-E [MD 2.7 (95% CI 0.1 to 5.3), p < 0.041] and the EORTC QLQ-OES18 pain scale [MD -2.2 (95% CI -4.3 to -0.2), p < 0.030]. Likewise, the effect estimates favored ICIs monotherapy arm over chemotherapy alone regarding QLQ-STO22 hair loss subscale [MD -23.2 (95% CI -29.7 to -16.7), p < 0.001], QLQ-STO22 dysphagia subscale [MD 6.7 (95% CI 1.7 to 11.7), p = 0.009], EQ-5D pain scale [MD 6.9 (95% CI 2.9 to 10.9), p < 0.001], and QLQ-OES18 saliva subscale [MD 5.8 (95% CI 0.1 to 11.6), p = 0.046].
Conclusions
In this meta-analysis, we found that the inclusion of ICIs for advanced GEC yielded better HRQoL outcomes than chemotherapy alone. Further research on the impact of ICIs on HRQoL is needed, with increasing evidence that ICIs improve survival outcomes in patients with advanced GEC.
Differential Analysis of Venous Sinus Diameters: Unveiling Vascular Alterations in Multiple Sclerosis
Authors: TUNÇ Abdulkadir , DANIŞAN Gürkan , TAYDAŞ Onur , KARA Ahmet Burak , ÖNCEL Samet , ÖZDEMİR Mustafa 
Published: February 2024

Abstract

Background
This study aims to explore the relationship between venous sinus diameters and Multiple Sclerosis (MS) to understand potential vascular alterations in MS patients compared to a control group. It seeks to determine if these alterations correlate with disease characteristics such as duration, lesion types, and disability scores.
Methods
The study included 79 MS patients, diagnosed according to the 2017 McDonald criteria, and 67 age- and gender-matched healthy individuals. MRI scans using a 1.5 Tesla system provided measurements of superior sagittal sinus, right and left transverse sinus, sinus rectus, and venous structures. Statistical analysis was conducted using SPSS, employing Independent Sample T-Test, ANOVA, Chi-Square, and Pearson Correlation Analysis, with a significance level set at p < 0.05.
Results
The study identified significant differences in venous sinus diameters between MS patients and the control group, with MS patients exhibiting larger diameters. Specifically, patients with brainstem and spinal lesions showed larger diameters in certain sinus regions. No significant correlations were found between venous sinus diameters and demographic factors, EDSS scores, or lesion counts. However, a significant increase in perivenular lesions was noted in patients with longer disease durations.
Conclusions
The findings indicate notable vascular alterations in MS patients, particularly in venous sinus diameters, suggesting a potential vascular component in MS pathology. The lack of correlation with conventional clinical and MRI metrics highlights the complexity of MS pathology. These insights underscore the need for further research, particularly longitudinal studies, to elucidate the role of venous changes in MS progression and their potential as therapeutic targets.