bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogeny and systematics of the “Pronophila clade,” with 2 new genera to resolve the formerly polyphyletic genus Pseudomaniola (Lepidoptera: Nymphalidae: Satyrinae)
Authors: Pyrcz Tomasz W , Willmott Keith R , Lachowska-Cierlik Dorota , Mrozek Artur , Florczyk Klaudia , Mahecha-J Oscar , Espeland Marianne 
Published: October 2023

Abstract
Analysis of a target enrichment molecular dataset confirms the monophyly of the Neotropical montane butterfly group known as the Pronophila Westwood clade, 1 of 2 major lineages of the satyrine subtribe Pronophilina. The Pronophila clade comprises 18–20 recognized genera and some 125 species. Within this group, the genus Pseudomaniola Röber appears as paraphyletic, and is split here into 3 genera, Pseudomaniola sensu novum with 6 species, including 4 previously considered as subspecies of P. phaselis (Hewitson), the monobasic Fahraeusia Pyrcz n. gen. for Catargynnis asuba Thieme, n. comb., and Boyeriana Pyrcz, Espeland & Willmott n. gen., with 9 species. The adults of all 3 genera can be recognized by their wing color patterns, but the strongest synapomorphies are found in the genitalia, especially those of the male, supporting the above systematic decisions. Notable differences are also found in scale organization and morphology. A divergence time analysis suggests that Fahraeusia diverged from Pseudomaniola + Boyeriana in the mid-Miocene, around 12 Mya, and the subsequent separation of the last 2 genera occurred at the start of the Pliocene at around 5 Mya.
Research on key technologies for intelligent and fine-grained construction of earth–rock dams based on artificial intelligence
Authors: Liu Biao , Gong Xiaohui , Meng Tao , Zhao Yufei 
Published: October 2023

Abstract
High dam construction is continuing to develop with new requirements for intelligent dam construction. New information technology capabilities are providing paths for improved intelligent dam construction. This paper provides a comprehensive overview of the key technologies employed in the intelligent construction process of earth–rock dams. Starting with the multidimensional perception technologies and equipment utilized in earth–rock dam filling construction, it covers various aspects, such as the fine-grained management of unit construction based on building information modeling, rapid detection and qualification testing of dam materials’ physical characteristics, fast perception technology for material compaction, and unmanned driving technology for dam compaction equipment. Additionally, this paper highlights an overview of the existing technical challenges and future prospects in intelligent construction for earth–rock dams. These research results provide important references and lessons for the construction and development of high earth–rock dam engineering.
Mutations in ompK36 differentially impact in vitro synergy of meropenem/vaborbactam and ceftazidime/avibactam in combination with other antibiotics against KPC-producing Klebsiella pneumoniae
Authors: Rogers Tara M , Kline Ellen G , Griffith Marissa P , Jones Chelsea E , Rubio Abigail M , Squires Kevin M , Shields Ryan K 
Published: October 2023

Abstract

Objectives
Ceftazidime/avibactam and meropenem/vaborbactam are preferred agents for Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kp) infections and are often used in combination with other agents. We aimed to characterize the synergy of combinations against KPC-Kp with varying ompK36 genotypes.

Methods
KPC-Kp that harboured ompK36 WT, IS5 or glycine-aspartic acid duplication (GD) genotypes were selected. MICs were determined in triplicate. Synergy was assessed by time-kill assays for ceftazidime/avibactam and meropenem/vaborbactam in combination with colistin, gentamicin, tigecycline, meropenem or fosfomycin against 1 × 108 cfu/mL KPC-Kp.

Results
KPC-Kp harboured ompK36 WT (n = 5), IS5 (n = 5) or GD (n = 5); 11 were KPC-2 and 4 were KPC-3. All were susceptible to ceftazidime/avibactam and meropenem/vaborbactam. In time-kill analysis, ceftazidime/avibactam and meropenem/vaborbactam 1 × MIC exhibited mean 24 h log-kills of −2.01 and −0.84, respectively. Ceftazidime/avibactam was synergistic in combination with colistin independent of ompK36 genotype. Ceftazidime/avibactam combinations impacted by porin mutations (compared to WT) were meropenem (−5.18 versus −6.62 mean log-kill, P &lt; 0.001) and fosfomycin (−3.98 versus −6.58, P = 0.058). Mean log-kills with meropenem/vaborbactam were greatest in combination with gentamicin (−5.36). In the presence of porin mutations, meropenem/vaborbactam killing activity was potentiated by the addition of colistin (−6.65 versus −0.70, P = 0.03) and fosfomycin (−3.12 versus 1.54, P = 0.003).

Conclusions
Our results shed new light on the synergy of ceftazidime/avibactam and meropenem/vaborbactam combinations against KPC-Kp with or without porin mutations. Killing activity of ceftazidime/avibactam with other cell wall active agents was decreased against isolates with porin mutations. On the other hand, some meropenem/vaborbactam combinations demonstrated enhanced killing in the presence of porin mutations.
Evaluating the incidence of ampC-β-lactamase genes, biofilm formation, and antibiotic resistance among hypervirulent and classical Klebsiella pneumoniae strains
Authors: Haddadi Mohammad Hossein , Khoshnood Saeed , Koupaei Maryam , Heidary Mohsen , Moradi Melika , Jamshidi Ali , Behrouj Hamid , Movahedpour Ahmad , Maleki Mohammad Hassan , Ghanavati Roya 
Published: October 2023

Abstract

Aim
Both immunocompetent and healthy individuals can become life-threateningly ill when exposed to the hypervirulent (hvKp) strains of Klebsiella pneumoniae (Kp). The main objectives of this study were to evaluate the presence of ampC-lactamase genes, biofilm formation, and antibiotic resistance in clinical strains of hvKp and cKp (classical K. pneumoniae).

Materials and methods
Kp strains were collected from patients referred to Shahidzadeh Hospital in Behbahan City, Khuzestan Province, Iran. Several techniques were used to identify hvKp. The hypermucoviscosity phenotype was determined using the string test. Isolates that developed dark colonies on tellurite agar were assumed to be hvKp strains. If any of the iucA, iutA, or peg-344 genes were detected, the isolates were classified as hvKp. Phenotypic and genotypic detection of AmpC β-lactamases of hvKp strains was performed by the combined disk method and polymerase chain reaction, respectively. In addition, crystal violet staining was used to determine the biofilm formation of these isolates.

Results
For this study, 76 non-duplicative isolates of Kp were collected. Overall, 22 (28.94%) strains had positive string test results, and 31 (40.78%) isolates were grown in tellurite-containing medium. The genes iucA and iutA or peg-344 were found in 23.68% of all Kp strains and in 50% of tellurite-resistant isolates, respectively. The most effective antibiotics against hvKp isolates were tetracycline (85.52%) and chloramphenicol (63.15%). Using the cefoxitin disc diffusion method, we observed that 56.57% (43/76) of the strains were AmpC producer. A total of 30.26% (n = 23/76) of the isolates tested positive for at least one ampC gene, including blaDHA (52.63%, n = 40), blaCIT (40.78%, n = 31), blaACC (19.76%, n = 15), blaMOX (25%, n = 19), and blaFOX (43.42%, n = 33). Biofilm formation analysis revealed that most hvKp isolates were weak (n = 6, 40%) and moderate (n = 5, 33.33%) biofilm producers.

Conclusion
Healthcare practitioners should consider the possibility of the existence and acquisition of hvKp everywhere. The exact mechanisms of bacterial acquisition are also unknown, and it is unclear whether the occurrence of infections is related to healthcare or not. Thus, there are still many questions about hvKp that need to be investigated.
Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil
Authors: de Oliveira Érica Maria , Beltrão Elizabeth Maria Bispo , Pimentel Maria Izabely Silva , Lopes Ana Catarina de Souza 
Published: October 2023

Abstract

Aims
Determine which sequence type (ST) clones were carrying the blaKPC, blaNDM, blaVIM, blaIMP, and blaGES genes and their variants in clinical isolates of multidrug-resistant Klebsiella pneumoniae.

Methods and Results
Ten K. pneumoniae isolates were obtained from the colonized and infected patients in a public hospital in the city of Recife-PE, in northeastern Brazil, and were further analyzed. The detection of carbapenem resistance genes and the seven housekeeping genes [for multilocus sequence typing (MLST) detection] were done with PCR and sequencing. The blaKPC and blaNDM genes were detected concomitantly in all isolates, with variants being detected blaNDM-1, blaNDM-5, blaNDM-7, and blaKPC-2. The blaKPC-2 and blaNDM-1 combination being the most frequent. Molecular typing by MLST detected three types of high-risk ST clones, associated with the clonal complex 258, ST11/CC258 in eight isolates, and ST855/CC258 and ST340/CC258 in the other two isolates.

Conclusions
These findings are worrying, as they have a negative impact on the scenario of antimicrobial resistance, and show the high genetic variability of K. pneumoniae and its ability to mutate resistance genes and risk of dissemination via different ST clones.
Improve coati optimization algorithm for solving constrained engineering optimization problems
Authors: Jia Heming , Shi Shengzhao , Wu Di , Rao Honghua , Zhang Jinrui , Abualigah Laith 
Published: October 2023

Abstract
The coati optimization algorithm (COA) is a meta-heuristic optimization algorithm proposed in 2022. It creates mathematical models according to the habits and social behaviors of coatis: (i) In the group organization of the coatis, half of the coatis climb trees to chase their prey away, while the other half wait beneath to catch it and (ii) Coatis avoidance predators behavior, which gives the algorithm strong global exploration ability. However, over the course of our experiment, we uncovered opportunities for enhancing the algorithm’s performance. When confronted with intricate optimization problems, certain limitations surfaced. Much like a long-nosed raccoon gradually narrowing its search range as it approaches the optimal solution, COA algorithm exhibited tendencies that could result in reduced convergence speed and the risk of becoming trapped in local optima. In this paper, we propose an improved coati optimization algorithm (ICOA) to enhance the algorithm’s efficiency. Through a sound-based search envelopment strategy, coatis can capture prey more quickly and accurately, allowing the algorithm to converge more rapidly. By employing a physical exertion strategy, coatis can have a greater variety of escape options when being chased, thereby enhancing the algorithm’s exploratory capabilities and the ability to escape local optima. Finally, the lens opposition-based learning strategy is added to improve the algorithm’s global performance. To validate the performance of the ICOA, we conducted tests using the IEEE CEC2014 and IEEE CEC2017 benchmark functions, as well as six engineering problems.
Undecidability of admissibility in the product of two Alt logics
Authors: Balbiani Philippe , Gencer Çiğdem 
Published: October 2023

Abstract
The product of two $\textbf {Alt}$ logics possesses the polynomial product finite model property and its membership problem is $\textbf {coNP}$-complete. Using a reduction from an undecidable domino-tiling problem, we prove that its admissibility problem is undecidable.
Neuroethics guidance documents: principles, analysis, and implementation strategies
Authors: O’Shaughnessy Matthew R , Johnson Walter G , Tournas Lucille Nalbach , Rozell Christopher J , Rommelfanger Karen S 
Published: October 2023

Abstract
Innovations in neurotechnologies have ignited conversations about ethics around the world, with implications for researchers, policymakers, and the private sector. The human rights impacts of neurotechnologies have drawn the attention of United Nations bodies; nearly 40 states are tasked with implementing the Organization for Economic Co-operation and Development’s principles for responsible innovation in neurotechnology; and the United States is considering placing export controls on brain-computer interfaces. Against this backdrop, we offer the first review and analysis of neuroethics guidance documents recently issued by prominent government, private, and academic groups, focusing on commonalities and divergences in articulated goals; envisioned roles and responsibilities of different stakeholder groups; and the suggested role of the public. Drawing on lessons from the governance of other emerging technologies, we suggest implementation and evaluation strategies to guide practitioners and policymakers in operationalizing these ethical norms in research, business, and policy settings.
DNA double-strand breaks enhance brassinosteroid signaling to activate quiescent center cell division in Arabidopsis
Authors: Takahashi Naoki , Suita Kazuki , Koike Toshiya , Ogita Nobuo , Zhang Ye , Umeda Masaaki 
Published: October 2023

AbstractIn Arabidopsis roots, the quiescent center (QC), a group of slowly dividing cells located at the center of the stem cell niche, functions as an organizing center to maintain the stemness of neighboring cells. Recent studies have shown that they also act as a reservoir for backup cells, which replenish DNA-damaged stem cells by activating cell division. The latter function is essential for maintaining stem cells under stressful conditions, thereby guaranteeing post-embryonic root development in fluctuating environments. In this study, we show that one of the brassinosteroid receptors in Arabidopsis, BRASSINOSTEROID INSENSITIVE1-LIKE3 (BRL3), plays a major role in activating QC division in response to DNA double-strand breaks. SUPPRESSOR OF GAMMA RESPONSE 1, a master transcription factor governing DNA damage response, directly induces BRL3. DNA damage-induced QC division was completely suppressed in brl3 mutants, whereas QC-specific overexpression of BRL3 activated QC division. Our data also showed that BRL3 is required to induce the AP2-type transcription factor ETHYLENE RESPONSE FACTOR 115, which triggers regenerative cell division. We propose that BRL3-dependent brassinosteroid signaling plays a unique role in activating QC division and replenishing dead stem cells, thereby enabling roots to restart growing after recovery from genotoxic stress.
Evaluation of TIB Molbiol LightMix® assays for detection of Mycoplasma genitalium and key resistance mutations for macrolides and fluoroquinolones
Authors: Balgovind Prisha , Atchison Steph , Danielewski Jennifer , Garland Suzanne M , Costa Anna-Maria , Bodiyabadu Kaveesha , Murray Gerald L 
Published: October 2023

Abstract
The LightMix® Modular Mycoplasma Macrolide and LightMix® Modular parC Fluoroquinolone Resistance assays (TIB Molbiol) were evaluated using sequential Mycoplasma genitalium positive (n = 125) and negative (n = 93) clinical samples. Results were compared to the results of an established commercial assay (ResistancePlus MG assay, SpeeDx Pty Ltd) or Sanger sequencing (for parC). Detection of M. genitalium by the TIB Molbiol assay had a high agreement with the reference assay, with a positive percent agreement (PPA) of 97.6 [95% confidence interval (CI): 93.1–99.5] and negative percent agreement (NPA) of 95.7 (95% CI: 89.5–98.8). From 105 positive samples, macrolide resistance detection had a PPA of 100% (95% CI: 93.7–100) and NPA of 81.3% (95% CI: 67.4–91.1). For the detection of fluroquinolone resistance mutation G248T/S83I or “other mutation” in the quinolone resistance determinant region, from 95 samples there was 100% (95% CI: 86.3–100) sensitivity and 100% (95% CI: 94.5–100) specificity. The understanding of the basis for fluoroquinolone treatment failure is still developing; it is therefore important to use the output of parC-based resistance assays with caution to avoid the inappropriate use of antibiotic therapies, especially considering the limited number of alternative treatments.
Imaging
Authors: Costa Guido , Lanza Ezio , Donadon Matteo , Torzilli Guido 
Published: October 2023

Abstract
Liver imaging represents an essential resource in the surgical treatment of liver diseases. Over the years, a shift has occurred in the role of liver imaging which has moved forward from the classical preoperative aetiological definition of a liver lesion. Nowadays, liver imaging aids the surgeon in the assessment of liver anatomy for optimal surgical planning, in the detection and characterization of focal liver lesions and biliary tract disorders in both the preoperative and intraoperative setting, and in the evaluation of the postoperative course and follow-up. This chapter provides an up-to-date review on the major imaging modalities that aid the surgeon in the surgical treatment of liver diseases.
Fatty liver in liver surgery
Authors: Goldaracena Nicolas , McCormack Lucas 
Published: October 2023

Abstract
Liver steatosis is considered as a clinically important feature that influences patient morbidity and mortality after hepatic resection and liver transplantation. Surgeons should take the presence of injured livers into account when planning the extent and type of hepatic surgery. Preoperative and perioperative interventions should be considered in these patients to minimize postoperative complications. Each multidisciplinary team should decide the best algorithm based on current evidence tailored to each patient to reach similar rates of morbidity and mortality compared to patients with healthy livers. Further randomized trials should focus on the evaluation of novel preoperative strategies, such as short-term intensive medical treatment of hepatic steatosis and preoperative portal vein embolization, to minimize risk in these patients.
Cystic lesions of the liver
Authors: Martin David , Melloul Emmanuel 
Published: October 2023

Abstract
Cystic lesions of the liver represent a heterogeneous group of fluid-filled lesions. Most of these lesions are benign and discovered incidentally. A minority can cause symptoms and rarely may be associated with complications. Hepatic cysts can be subdivided into simple and complex cysts, based on their characteristics. Ultrasound is typically the first-line imaging modality. Computed tomography scanning or magnetic resonance imaging gives more detailed information about location, surrounding structures, nodularity, septations, and calcification within the cyst. There are still controversies about the definitions and classifications of cysts. Furthermore, no consensus has been achieved on the optimal treatment of patients with symptoms. This chapter provides an overview of the diagnosis and management of the most commonly observed hepatic cystic lesions.
Primary neoplasms of the liver
Authors: Muiesan Paolo , Schlegel Andrea , Grimaldi Chiara , Bici Kejd 
Published: October 2023

Abstract
Liver tumours represent around 2% of paediatric solid tumours. Among liver malignancies hepatoblastoma is the most frequent (80%), followed by hepatocellular carcinoma and other rare tumours. In the last decade, the management of hepatoblastoma has significantly evolved, leading to constant improvements in long-term outcome. In fact, the disease-free survival has increased from 30% in the prechemotherapy era to 70–80% in recent reports. As far as complete tumour resection remains the standpoint of treatment, multimodality and multispecialist management is the key for the constant amelioration of results. New cooperative groups like the Children’s Hepatic tumours International Collaboration (CHIC) allowed the collection of data from large series to introduce a new, simplified risk staging system and more data will be obtained with the institution of the Pediatric Hepatic International Tumor Trial (PHITT) protocol to optimize chemotherapy treatments. In this chapter, pre- and intraoperative management of HB are detailed, along with the most recent advances and challenges in the approach to patients with HB to give a complete and deep overview of the topic.
Non-operative management of liver lesions
Authors: Deepa Shree T. , Botcha Sandeep 
Published: October 2023

Abstract
Liver malignancies are the second leading cause of cancer-related mortality worldwide. Transplantation and surgical resection of the liver remain the gold standard curative treatment options; however, a limited number (15–20%) of patients are suitable candidates for these procedures due to advanced liver disease and associated comorbidities. A variety of image-guided percutaneous ablative and catheter-based locoregional techniques have been developed and intensively investigated in patients with inoperable primary and secondary liver lesions. They are useful to downstage and bridge patients for surgical and transplant interventions, definitive curative intent in smaller lesions, and as primary palliative management improving survival outcomes and quality of life with increasing importance in treating postsurgical tumour recurrence. This chapter summarizes various currently available non-operative minimally invasive interventional oncology treatments along with their techniques of use in planning and managing liver lesions.
Endoscopic diagnostic and treatment modalities of the biliary system
Authors: Gubler Christoph , Oliver Frans 
Published: October 2023

Abstract
Parallel to the evolution of radiological imaging, endosonography and endoscopic retrograde cholangiopancreatography (ERCP) have evolved into important diagnostic and therapeutic modalities in biliary disease. ERCP has shifted to an almost solely therapeutic intervention whereas endosonography gained grounds in both diagnostics and therapy. Beside characterizing and staging lesions, endosonography allows acquisition of tissue to confirm a diagnosis. Therapeutically, endosonography enables gaining access to the biliary system in altered anatomy or when other methods fail. ERCP remains the intervention of choice in stone and most stricturing bile duct diseases regardless of the aetiology. Through-the-endoscope (dilatation) balloons, baskets, stents, radiofrequency ablation catheters, cholangioscopes, and electrohydraulic/laser lithotripsy catheters have expended the therapeutic possibilities.
Acute and chronic cholecystitis
Authors: Goumard Claire , Scatton Olivier 
Published: October 2023

Abstract
In the era of minimally invasive surgery, cholecystitis remains the main risk factor for bile duct injury during cholecystectomy, irrespective of the surgical approach—laparoscopic or open. This chapter reviews, according to the most recent recommendations, the indications, surgical technique, and alternative strategies in the case of cholecystitis depending on its severity. It emphasizes the importance of obtaining the critical view of safety, and describes the recommended ‘bail-out’ procedures in case it is impossible to safely obtain the critical view of safety. Dome-down cholecystectomy or partial cholecystectomy must be considered early to prevent any bile duct or vascular injury in case of severe inflammation. Open conversion remains a safe option in case of unclear anatomy, but does not always prevent biliary or vascular injuries, as the experience of surgeons has shifted from open to laparoscopic techniques in the last decades.