bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR615‐3p inhibited FBLN1 and osteogenic differentiation of umbilical cord mesenchymal stem cells by associated with YTHDF2 in a m6A‐miRNA interaction manner
Authors: Yang Haoqing , Wang Wanqing , Liu Huina , Zhang Chen , Cao Yangyang , Long Lujue , Han Xiao , Wang Yuejun , Yan Fei , Li Guoqing , Zhu Mengyuan , Jin Luyuan , Fan Zhipeng 
Published: February 2024

AbstractTo investigate the role and mechanism of FBLN1 in the osteogenic differentiation and bone regeneration by using umbilical cord mesenchymal stem cells (WJCMSCs). We found that FBLN1 promoted osteogenic differentiation of WJCMSCs and WJCMSC‐mediated bone regeneration. It was showed that there was an m6A methylation site in 3′UTR of FBLN1 mRNA, and the mutation of the m6A site enhanced the stability of FBLN1 mRNA, subsequently fostering the FBLN1 enhanced osteogenic differentiation of WJCMSCs. YTHDF2 was identified as capable of recognizing and binding to the m6A site, consequently inducing FBLN1 instability and repressed the osteogenic differentiation of WJCMSCs. Meanwhile, miR‐615‐3p negatively regulated FBLN1 by binding FBLN1 3′UTR and inhibited the osteogenic differentiation of WJCMSCs and WJCMSC‐mediated bone regeneration. Then, we discovered miR‐615‐3p was found to regulate the functions of FBLN1 facilitated by YTHDF2 through an m6A‐miRNA regulation mechanism. We demonstrated that FBLN1 is critical for regulating the osteogenic differentiation potentials of WJCMSCs and have identified that miR615‐3p mediated the decay of FBLN1 mRNA which facilitated by m6A reading protein YTHDF2. This provided a novel m6A‐miRNA epigenetic regulatory pattern for MSC regulation and bone regeneration.
Updates in head and neck cytopathology: Insights from European Congress of Pathology Short Course
Authors: Kholová Ivana , Chandra Ashish , Faquin William C. , Rupp Niels J. , Touska Philip , O'Regan Esther 
Published: February 2024

AbstractCytological specimens play a pivotal role in head and neck nodule/mass work up and diagnoses. The specimens´ importance has grown with the onset of personalized medicine and the routine use of molecular markers in the diagnostic work up. The Updates in Head and Neck Cytopathology Short Course ran during the 35th European Congress of Pathology held in Dublin, Ireland, in 2023 and brought together experts in cytopathology, pathology, and related fields to share their expertise and experience in the field of head and neck cytopathology and its future directions. Topics such as a one‐stop clinic, the Milan System for Reporting Salivary Gland Cytopathology, next generation sequencing, and human papilloma virus detection in the head and neck area were covered during the short course. These topics are briefly summarized in the present review.
Hypothalamic control of body fat mass by food intake: The key to understanding why obesity should be treated as a disease
Authors: Purnell Jonathan Q. , le Roux Carel W. 
Published: February 2024

AbstractBackgroundHypothalamic centres have been recognized to play a central role in body weight regulation for nearly 70 years.AimsIn this review, we will explore the current undersanding of the role the hypothalamus plays in controlling food intake behaviours.Materials and MethodsReview of relevant literature from PubMed searches and review article citations.ResultsBeginning with autopsy studies showing destructive hypothalamic lesions in patients manifesting hyperphagia and rapid weight gain, followed by animal lesioning studies pinpointing adjacent hypothalamic sites as the ‘satiety’ centre and the ‘feeding’ centre of the brain, the neurocircuitry that governs our body weight is now understood to consist of a complex, interconnected network, including the hypothalamus and extending to cortical sites, reward centres and brainstem. Neurons in these sites receive afferent signals from the gastrointestinal tract and adipose tissue indicating food availability, calorie content, as well as body fat mass.DiscussionIntegration of these complex signals leads to modulation of the two prime effector systems that defend a body fat mass set point: food intake and energy expenditure.ConclusionUnderstanding the hypothalamic control of food intake forms the foundation for understanding and managing obesity as a chronic disease.
ACAD10 is not required for metformin's metabolic actions or for maintenance of whole‐body metabolism in C57BL/6J mice
Authors: Yew Michael J. , Heywood Sarah E. , Ng Joe , West Olivia M. , Pal Martin , Kueh Andrew , Lancaster Graeme I. , Myers Stephen , Yang Christine , Liu Yingying , Reibe Saskia , Mellett Natalie A. , Meikle Peter J. , Febbraio Mark A. , Greening David W. , Drew Brian G. , Henstridge Darren C. 
Published: February 2024

AbstractAimAcyl‐coenzyme A dehydrogenase family member 10 (ACAD10) is a mitochondrial protein purported to be involved in the fatty acid oxidation pathway. Metformin is the most prescribed therapy for type 2 diabetes; however, its precise mechanisms of action(s) are still being uncovered. Upregulation of ACAD10 is a requirement for metformin's ability to inhibit growth in cancer cells and extend lifespan in Caenorhabditis elegans. However, it is unknown whether ACAD10 plays a role in metformin's metabolic actions.Materials and MethodsWe assessed the role for ACAD10 on whole‐body metabolism and metformin action by generating ACAD10KO mice on a C57BL/6J background via CRISPR‐Cas9 technology. In‐depth metabolic phenotyping was conducted in both sexes on a normal chow and high fat‐high sucrose diet.ResultsCompared with wildtype mice, we detected no difference in body composition, energy expenditure or glucose tolerance in male or female ACAD10KO mice, on a chow diet or high‐fat, high‐sucrose diet (p ≥ .05). Hepatic mitochondrial function and insulin signalling was not different between genotypes under basal or insulin‐stimulated conditions (p ≥ .05). Glucose excursions following acute administration of metformin before a glucose tolerance test were not different between genotypes nor was body composition or energy expenditure altered after 4 weeks of daily metformin treatment (p ≥ .05). Despite the lack of a metabolic phenotype, liver lipidomic analysis suggests ACAD10 depletion influences the abundance of specific ceramide species containing very long chain fatty acids, while metformin treatment altered clusters of cholesterol ester, plasmalogen, phosphatidylcholine and ceramide species.ConclusionsLoss of ACAD10 does not alter whole‐body metabolism or impact the acute or chronic metabolic actions of metformin in this model.
Risk factors for homelessness and housing instability in the first episode of mental illness: Initial findings from the AMONT study
Authors: Roy Laurence , Leclair Marichelle , Crocker Anne G. , Abdel‐Baki Amal , de Benedictis Luigi , Bérubé Felix‐Antoine , Thibeault Esther , Latimer Eric , Roy Marc‐André 
Published: February 2024

AbstractAimPeople living with mental illness are more likely than the general population to experience adverse housing outcomes, including homelessness. The aim of the current study is to examine residential status when participants have their first contact with mental health services, and the correlates of residential status at that moment.MethodsFirst‐time mental health service users were recruited from seven clinical sites across Québec. Data on residential status at entry in the project, as well as demographic, clinical and social variables, were collected using self‐report and interviewer‐rated questionnaires. Participants were classified as ‘Homeless’, ‘At risk of homelessness’ and ‘Stably Housed’, and correlates of residential status were identified through multivariate logistic regression and unbiased recursive partitioning.ResultsAmong the 478 participants, 206 (43.1%) were in stable housing, 171 (35.8%) were at risk of homelessness and 101 (21.1%) were classified as homeless. Placement in a youth protection facility was strongly associated with adverse housing outcomes, while having a high school diploma and more social support were associated with more stable housing situations.ConclusionsFirst‐time mental health service users are likely to experience a range of adverse housing situations, indicating the potential for clinical sites to implement homelessness primary prevention strategies. Factors related to family, foster care and schooling seem to be particularly salient in understanding risk of homelessness in first‐time mental health service users, calling for intersectoral action to prevent adverse psychosocial outcomes in this population.
Anticipating future threats: Evidence for association, but not interaction, of childhood adversity and identity development with threat anticipation in adolescence
Authors: Samaey Celine , Lecei Aleksandra , Achterhof Robin , Hagemann Noëmi , Hermans Karlijn S. F. M. , Hiekkaranta Anu P. , Kirtley Olivia J. , Reininghaus Ulrich , Boets Bart , Myin‐Germeys Inez , van Winkel Ruud 
Published: February 2024

AbstractAimChildhood adversity may result in a negative expectation of future interactions with others, also referred to as ‘threat anticipation’. It may also negatively impact on identity development, which subsequently may influence how individuals deal with their environment. Here, we examine the hypotheses that (1) identity synthesis is associated with reduced anticipation of threat, whereas the opposite would be true for identity confusion, and (2) that identity confusion exacerbates the association between childhood adversity and threat anticipation.MethodsOne thousand nine hundred and thirteen adolescents from the general population (mean age = 13.8 years, SD = 1.86, range = 11–20) completed self‐report questionnaires assessing exposure to childhood adversity, identity development and threat anticipation.Results and DiscussionIdentity development was significantly associated with threat anticipation in the expected direction: identity synthesis was associated with reduced anticipation of threat (β = −.0013, p < .001), whereas identity confusion was association with increased threat anticipation (β = .0017, p < .001). Furthermore, childhood adversity was positively associated with threat anticipation (β = .0018, p < .001). However, no evidence for an interaction effect of identity on the association between childhood adversity and threat anticipation was found, suggesting childhood adversity and identity development have an independent rather than synergistic effect on threat anticipation.ConclusionThe current study illustrates the importance of exposure to childhood adversity and identity development for threat anticipation in adolescence. Further research is needed to clarify how both factors influence each other within a developmental framework.
Identification of distinct clinical profiles and trajectories in individuals at high risk of developing psychosis: A latent profile analysis of the north American prodrome longitudinal study consortium‐3 dataset
Authors: Bergé Daniel , Carter Cameron S. , Smucny Jason 
Published: February 2024

AbstractAimPeople at clinical high risk (CHR) for psychosis are a heterogeneous population in regard to clinical presentation and outcome. It is unclear, however, if their baseline clinical characteristics can be used to construct orthogonal subgroups that differ in their clinical trajectory to provide early identification of individuals in need of tailored interventions.MethodsWe used latent profile analysis (LPA) to determine the number of distinct clinical profiles within the CHR population using the NAPLS‐3 dataset, focusing on the clinical features incorporated in the NAPLS psychosis risk calculator (including age, unusual thought content and suspiciousness, processing speed, verbal learning and memory function, social functioning decline, life events, childhood trauma, and family history of psychosis). We then conducted a between‐profile comparisons of clinical trajectories based on psychotic and depressive symptoms as well as substance use disorder (SUD) related features over time.ResultsTwo distinct profiles emerged. One profile, comprising approximately 25% of the sample, was significantly older, displayed better cognitive performance, experienced more types of traumatic and undesirable life events, exhibited a greater decline in functioning in the past year, and was more likely to have relatives with psychosis. This group showed worse positive symptoms and SUD‐related features over time, although groups did not differ in the proportion of individuals who developed psychosis.ConclusionsLPA results suggest CHRs can be segregated into two profiles with different clinical trajectories. Characterizing individuals within these clinical profiles may help understand the divergent outcomes of this population and ultimately facilitate the development of specialized interventions.
Benzodiazepines for pediatric epilepsies and their risks in a cohort within the French health care data
Authors: Auvin Stéphane , Guillo Sylvie , de Rycke Yann , Tran Diep , Tubach Florence 
Published: February 2024

AbstractObjectiveThe management of antiseizure treatment in patients with epilepsy relies on the benefit–risk ratio. Data on antiseizure medication (ASM) use in children are limited. We described antiseizure medication use in children with epilepsy (CwE) in France, with a focus on the chronic use of benzodiazepines and related implications.MethodsWe conducted a 5‐year cohort study from January 2012, using data from the French national health care data system (Système National des Données de Santé). We included CwE identified through International Classification of Diseases, 10th Revision codes and medications from January 2012 to December 2015 and followed them until December 2016. We described ASMs and assessed whether the risk of initiating a polytherapy after a bitherapy depends on whether benzodiazepine was included in the bitherapy.ResultsWe identified 62 885 CwE. Valproate was the most reimbursed ASM (40%), followed by lamotrigine (17.6%), levetiracetam (9.3%), clobazam (6.1%), and carbamazepine (5.8%). Prescriptions were initiated at the hospital in 74.5% of CwE. We observed a decrease in the number of CwE with at least one benzodiazepine reimbursement from 15.3% in 2013 to 10.1% in 2016 (p < .0001). The prevalence of CwE with levetiracetam reimbursements increased, whereas that of CwE with valproate decreased. A switch from a bitherapy to a polytherapy was more likely when the bitherapy included a benzodiazepine (subdistribution hazard ratio [sHR] = 1.20 [1.03–1.39]).SignificanceThe prevalence of CwE with at least one benzodiazepine reimbursement decreased during the study period. Benzodiazepines were associated with an increased use of subsequent ASM polytherapy.
A nonlinear lifetime prediction method for un‐ and low alloyed steels by damage determination based on non‐destructive measurement techniques
Authors: Weber Fabian , Maul Moritz , Juner Florian , Starke Peter 
Published: February 2024

AbstractMaterials used in nuclear power plants are exposed to cyclic loading, which is why the understanding of fatigue behaviour is of great importance. Because of high time investments and resulting increased costs, Lifetime Prediction Methods, which significantly reduce the amount of specimen, have already been developed. Within this paper, the Lifetime Prediction Method MiDAcLifeincr is presented, which enables a determination of a trend S‐N curve in the High‐Cycle‐Fatigue‐regime based on only one fatigue test and the combination of conventional fatigue testing with NDT‐related measurement techniques. The process‐orientated monitoring of the cyclic deformation behaviour provides the basis for the time‐ and cost‐effective provision of fatigue data. Compared with previous methods, the consideration of variable loading is an essential aspect of the research application. The test material is a 20MnMoNi5‐5 steel, the results of which are extended to include other steels for validation reasons.
Comparative evaluation of fatigue life estimation under variable amplitude loading through damage models based on entropy
Authors: Rodríguez‐Reyes Víctor I. , Abúndez‐Pliego Arturo , Petatan‐Bahena Kevin E. , Miranda‐Acatitlan Kevin R. , Colín‐Ocampo Jorge , Blanco‐Ortega Andrés 
Published: February 2024

AbstractThree fatigue damage models based on entropy, originally developed for constant amplitude loading, were assessed and compared to each other aimed at stating whether its applicability can be extended towards variable amplitude loading conditions. A variable amplitude loading history, applied on a 2024‐T3 aluminum alloy reported in the literature, was processed using both rainflow cycle counting and spectral techniques to transform it into a distribution of simple processes with constant amplitudes, then the damage models were assessed under the new loading conditions. The results showed that the model by Khonsari, combined with the rainflow technique, exhibited the highest accuracy with regard to the referenced experimental results with a −0.67 standard deviation from the average data and a 16% error from the median. Therefore, it is possible to assess the fatigue damage accumulation in metallic materials under variable amplitude loading through a thermodynamic approach with models developed for constant amplitude loading.
Fish aggregations at oil and gas platform foundations in the North Sea
Authors: Ibanez‐Erquiaga Bruno , Baktoft Henrik , Wilms Tim , Mildenberger Tobias Karl , Svendsen Jon Christian 
Published: February 2024

AbstractGlobally, aging offshore oil and gas (O&G) platforms face resource depletion, and regulations often demand platform removal through decommissioning. In the North Sea, >€90 billion will be needed for decommissioning by 2060. However, the influence of O&G platforms on fish communities is poorly understood. This challenges predictions of possible fisheries scenarios associated with different decommissioning options. Here, we examined the role that North Sea platforms played on fish communities. We explored patterns of fish abundance and body size variation along transects from O&G platforms comparing catch per unit effort data. Fish abundance was positively associated with O&G platforms. Atlantic cod, plaice, saithe, and mackerel were most abundant near platforms. Similarly, larger Atlantic cod were near the O&G platforms. Our study provided information for decision‐making regarding platform decommissioning scenarios by evidencing O&G platform habitat provisioning for various commercial fish species.
A warming western boundary current increases the prevalence of commercially disruptive parasites in broadbill swordfish
Authors: Bolin Jessica A. , Evans Karen , Schoeman David S. , Spillman Claire M. , Moore Thomas S. , Hartog Jason R. , Cummins Scott F. , Scales Kylie L. 
Published: February 2024

AbstractMeat quality is of paramount importance in the fisheries and aquaculture industries, but the quality of seafood can be affected by environmental variability and change, creating uncertainties in the delivery of consistent, high‐end product. Reports from fishers operating in an Australian fishery catching broadbill swordfish (Xiphias gladius) suggest that anomalously warm waters are linked with myoliquefaction of muscle tissue. The condition affects the marketability of fish by turning the meat into a soft, mushy texture post‐mortem and is caused by infection by the myxozoan parasite Kudoa musculoliquefaciens. Here, we combine microscopy, molecular techniques and ecological modelling to explore potential environmental drivers of the prevalence and intensity of K. musculoliquefaciens in swordfish, as a first step in understanding how a warming ocean might exacerbate the risk of harvesting an infected swordfish and the resultant potential risk of myoliquefaction. We develop predictive dynamic risk surfaces on seasonal timescales, with results revealing both the likelihood of harvesting an infected swordfish and the intensity of parasite load increase during the Austral summer. The prevalence of the parasite further increases in the region dominated by the East Australian Current, when locally warm areas are atypically cool and when average monthly temperatures are more variable. These findings provide information useful in predicting the conditions under which the risk of harvesting infected swordfish might be intensified, enabling adaptation to climate change impacts and optimisation of decision‐making when fishing under risky conditions.
Bird species' tolerance to human pressures and associations with population change
Authors: Marjakangas Emma‐Liina , Johnston Alison , Santangeli Andrea , Lehikoinen Aleksi 
Published: February 2024

AbstractAimSome species thrive in human‐dominated environments, while others are highly sensitive to all human pressures. However, standardized estimates of species' tolerances to human pressures are lacking at large spatial extents and taxonomic breadth. Here, we quantify the world's bird species' tolerances to human pressures. The associated precision values can be applied to scientific research and conservation.LocationGlobal.Time Period2013–2021.Major Taxa Studied6090 bird species.MethodsWe used binary observation data from eBird and modelled species' occurrences as a function of the Human Footprint Index (HFI). With these models, we predicted how likely each species was to occur under different levels of human pressures. Then, we calculated each species' Human Tolerance Index (HTI) as the level of the HFI where predicted occurrence probability was reduced to 50% of the maximum species' occurrence probability. We used resampling to obtain estimates of uncertainty of the Human Tolerance Indices. We also compared tolerances across species with increasing, stable, and decreasing population trends.ResultsWe found that 22% of the bird species tolerated the most modified human‐dominated environments, whereas 0.001% of species only occurred in the intact environments. We also found that HTI varied according to species' population trend categories, whereby species with decreasing population trends had a lower tolerance than species with increasing or stable population trends.Main ConclusionsThe estimated HTI indicates the potential of species to exist in a landscape of intensifying human pressures. It can identify species unable to tolerate these environments and inform subsequent conservation efforts. We found evidence that species' sensitivity to human‐dominated environments may be driving birds' use of space. Bird species' tolerances are also linked to their population trends, making the tolerances a relevant addition to conservation planning.