bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide
Authors: Wang Xiao-Li , Zhang Ru-Nan , Pan Yu-Lin , Li Zhi-Ming , Li Hong-Qiu , Lei Ya-Ting , Zhao Fang-Fang , Hao Xiao-Xiao , Ma Wei-Wei , Yu Cui-Ping , Yao Hong-Wei , Wang Xin-Yu , Lv Jun-Jie , Wu Yong-Hui , Wang Sheng-Yuan 
Published: February 2024

Abstract
The impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear. We used proteomics technology to analyze the serum of the ACR occupationally exposed population and screen out target proteins related to learning and memory. Through in vivo, in vitro experiments, and transgenic mouse models, we investigated the selected target protein to elucidate. Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomic. Results from in vitro and in vivo experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR (P < 0.05). Subsequent in vitro experiments using eEF2K siRNA and in vivo experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits (P < 0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. KEGG analysis indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism. In summary, eEF2K as a pivotal target in the mechanisms underlying ACR-induced learning and memory impairment, providing robust evidence for potential clinical interventions targeting.
Enhancing Personalized Recommendations via Multimodal Cooperative Learning
Authors: Jimenez Citlali , Velasco Adriana , Campbell Winona 
Published: February 2024

Abstract
Personalized recommender systems represent intelligent algorithms and decision-making mechanisms that are meticulously crafted on the bedrock of extensive datasets. In the realm of online recommender systems, the process involves tailoring recommendations to individual users by harnessing their historical behavioral data, which encompasses activities such as browsing, watching, purchasing, and rating. Simultaneously, these systems delve into the content information associated with the items on offer, including introductory details, specific features, and applicable scenarios. The essence of personalized recommendations lies in the continuous refinement of the system's output based on user interaction feedback. In recommender systems, understanding the relationships between items is crucial. We introduce a sophisticated multimodal cooperative learning strategy that explicitly captures the interconnections among diverse modalities, elevating the representation quality of each modality.
Voice assessment in patients with amyotrophic lateral sclerosis: Association with bulbar and respiratory function
Authors: Rocha Pedro Santos , Bento Nuno , Svard Hanna , Candeias Diana Nicolau , Hespanhol Sandra , Folgado Duarte , Carreiro André Valério , de Carvalho Mamede , Miranda Bruno 
Published: February 2024

Abstract

Patients with amyotrophic lateral sclerosis (ALS) face respiratory and bulbar dysfunction causing profound functional disability. Speech production requires integrity of bulbar muscles and good breathing capacity, being a possible way to monitor such functions in ALS. Here, we studied the relationship between bulbar and respiratory functions with voice characteristics of ALS patients, at the convenience of using a simple smartphone for voice recordings. For voice assessment we considered a speech therapists’ standardized tool – Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V); and an acoustic analysis toolbox (for both time and frequency domains). The bulbar subscore of the revised ALS Functional Rating Scale (ALSFRS-R) was used; and pulmonary function measurements included forced vital capacity (FVC%) and maximum expiratory and inspiratory pressures (MIP% and MEP%, respectively). Correlation coefficients and both linear and logistic regression models were applied. A total of 27 ALS patients (12 males; 61 years mean age; 28 months median disease duration) were included. Patients with significant bulbar dysfunction revealed greater CAPE-V scores in overall severity, roughness, strain, pitch, and loudness. They also presented slower speaking rates, longer pauses, and higher jitter values in acoustic analysis (all p < 0.05). The CAPE-V’s overall severity and sub-scores for pitch and loudness demonstrated significant correlations with MIP% and MEP% (all p < 0.05). By contrast, acoustic metrics (speaking rate, absolute energy, shimmer, and harmonic-to-noise ratio) were significantly correlated with FVC% (all p < 0.05).The results provide supporting evidence for using smartphone-based recordings in ALS patients for CAPE-V and acoustic analysis as good correlates of bulbar and respiratory function.
The glass transition temperature of anhydrous amorphous calcium carbonate
Authors: Bissbort Thilo , Hess Kai-Uwe , Wilding Martin , Schawe Jürgen E.K. , Purgstaller Bettina , Goetschl Katja E. , Sturm Sebastian , Müller-Caspary Knut , Sturm Elena V. , Schmahl Wolfgang , Griesshaber Erika , Weidendorfer Daniel , Dietzel Martin , Dingwell Donald B. 
Published: February 2024

Abstract
Amorphous calcium carbonate (ACC) is the least stable polymorph of calcium carbonates. It has been identified to play an important role in nature (e.g., biomineralization and speleothem formation), where it acts as a precursor for the transformation to more stable polymorphs such as calcite. Furthermore, the use of ACC in technical applications requires a robust understanding of the material’s properties. We present the first study that reveals the existence of a glass transition for synthetic and anhydrous ACC. The glass transition occurs at 339 °C. Such measurements are impossible with conventional differential scanning calorimetry (DSC) due to the high tendency of ACC to crystallize. Fast scanning DSC with heating rates of 500 °C/s and higher, however, can be used to separate the endothermic glass transition signature from the exothermic crystallization event since crystallization is shifted to higher temperatures. This allows the detection and quantification of the glass transition for ACC. These observations indicate that ACC is a structural glass and are especially significant because the synthesis of ACC, precipitation from a solution followed by lyophilization, contrasts with the more conventional and well-known route of glass formation–the rapid cooling of a melt. Moreover, we prove that a structural glass can be produced from a simple single-component carbonate system.
A REVIEW OF KOMBUCHA ANTIOXIDANT EFFECTS ON ATHEROSCLEROSIS IN PRECLINICAL STUDIES
Authors: Elizabeth  , Amabel Sharone , Ardo Sanjaya  , Julia Windi Gunadi  , Diana Krisanti Jasaputra  
Published: February 2024

Background Atherosclerosis is the leading cause of death among cardiovascular diseases in Indonesia, in which death is possible if uncontrolled. The prevalence of the disease may be prevented by consuming foods or drinks containing high levels of antioxidant such as kombucha. Exploration of kombucha properties as an antioxidant that will improve atherosclerosis could be obtained from preclinical studies.
Objective To discuss the antioxidant effect of kombucha on atherosclerosis in preclinical studies.
Method This study is a narrative review with literatures from 2013 to 2022.
Result Kombucha is an antioxidant-rich beverage made from enzymes generated by the SCOBY bacteria, which are fermented in sweet tea for 7-14 days to achieve optimal quantities of polyphenols. In addition to the antioxidants in the blood, the effect will lower the levels of LDL, VLDL, triglycerides, and total cholesterol in the blood. Moreover, it can increase HDL in blood and inhibit lesion forming in the aorta.
Conclusion Polyphenol in kombucha tea has an antiatherogenic effect that may prevent atherosclerosis in preclinical studies.
Keywords: antioxidant, atherosclerosis, kombucha, polyphenol
Research on the joint adjustment model of regional water resource network based on the network flow theory
Authors: Ye Zhou , Ding Lin , Liu Zhisong , Chen Fang 
Published: February 2024

Abstract
This study uses the network flow theory to optimize regional water resource allocation. In order to solve the problem of inefficient utilization of water resources with decentralized decision-making by different administrative units, a regional water resource networking and joint dispatching model with multi-objective nonlinear characteristics based on the network flow theory (hereinafter referred to as the network flow model) is constructed in the study. The network flow model was simulated and applied in the Xin-Sheng area of the Cao'e River, a tributary of the Qiantang River, and the results of the study showed that the network flow model scheduling increased significantly in efficiency compared with the current conventional scheduling, with an increase of 35.24 and 9.91% in the water resource utilization rate in the two typical years of 2019 and 2022, respectively, and showed that 2022, which has less rainfall, has a better effect than 2019. The study concludes that the network flow model can effectively improve the efficiency of water resource utilization, solve the problem of water resource imbalance between cities in the region, and play a positive role in the construction of the national water network.
Application of a multiparameter sonde for real-time monitoring of seawater quality in Durrës Bay in Albania
Authors: Marku Elda , Tahiraj Jonida , Lazo Pranvera , Drushku Spiro , Qarri Flora , Nuro Aurel , Myrtaj Bledar 
Published: February 2024

Abstract

Coastal areas are characterized by a high population and a wide range of industrial and agricultural activities, which puts them under high pressure and continuous pollution from anthropogenic activities. This research focuses on the application of HYDROLAB HL7 multiparameter sonde equipped with smart sensors for the measurement of physical–chemical parameters in marine waters in the Durrës Bay. The sonde is part of a transnational repository network that receives, stores, and analyzes data about seawater quality, serving as an early warning system for preventing the diffusion of marine pollution. This sophisticated instrument can thrive in demanding environmental conditions for long-term continuous monitoring. It maximizes deployment lifespan, and provides traceable data for high-quality, reliable monitoring of vital changes in water quality. Low variability on the measured parameters indicates a stable status in the water quality of the Durrës site. Time series revealed small seasonal variations on all parameters, except turbidity and water temperature. Total dissolved solids, salinity, and electrical conductivity revealed similar temporal trends over the monitoring period by indicating strong relationships between them. The obtained data for the physical–chemical parameters in this study align with the recommended values. Ensuring water quality in the Durrës Bay requires advanced monitoring, regulatory measures, and community engagement.
Detection of endotoxins from selected drinking water microbiota using an LAL-based assay and its implications for human health
Authors: Hawer Harmen , Burmester Rebecca , Sonnenberg Nadine , Weiß Katja 
Published: February 2024

Abstract
Endotoxins are pyrogenic lipopolysaccharides from Gram-negative bacteria that are known to induce fever, septic shock, and multiple organ failure, posing a substantial risk to human health. Drinking water systems are especially prone to home microbiomes containing a large variety of Gram-negative bacteria. Consumption of water from these systems in developed countries is generally regarded as non-hazardous to humans due to the low number of non-pathogenic bacterial cells per milliliter and oral admission. To assess potential risks posed by endotoxins in drinking water systems, we conducted a conventional microbiological investigation on a local community water system in the north of Germany and mined the resulting data to investigate the endotoxin contents of some of the most abundant microbiota found during these analyses. Using a Limulus amoebocyte lysate (LAL) -based endotoxin detection method, average normalized endotoxin content was determined. Although the average culturable amounts of microbiota in the drinking water system were insufficient to exert endotoxin levels critical to human health, peaks and acute contaminations may pose substantial health risks.
Integrated assessment of future climate and land use changes on urban floods: A Markov chain and PCSWMM-based approach for Hyderabad case study
Authors: Sagar Kumar Manchikatla , Umamahesh N. V. 
Published: February 2024

Abstract

This research examines the impact of climate change and urban expansion on urban drainage systems in Hyderabad (Zone-XII, Zone-IV&V), India. It employs a Markov chain-based framework to simulate future climate and land changes. Integrated 1D-2D PCSWMM model is used to assess the hazards posed by these changes. Present and future extreme rainfall event(s) (1–10 days) are simulated to determine maximum flooding hours, valuable for resilience studies. Future rainfall events are simulated under four SSP scenarios using CMIP6 Global Climate Models (GCMs): EC-Earth3-Veg, MPI-ESM-1-2-HR, and MPI-ESM-1-2-LR. The Markov Chain Precipitation Generator (MCPG) model downscales grid-scale precipitation data to station-scale. Future urban land expansion is simulated using the Markov Chain-Cellular Automata (MC-CA) model with Terrset. MCPG model is validated using performance measures, and it showed most increased rainfall events under EC-Earth3-Veg. The MC-CA model obtained a Kappa coefficient of 0.89, indicating an increase in imperviousness in future LULC; 6.1% of vegetation and 29.06% of barren land in 2022 will be urbanized by 2075. A significant increase in extreme flood hazard areas for the 1-day and above 7-day events in the both zones is observed from the PCSWMM results. The study highlighted the importance of Markov chains and event duration in flood hazard assessments.
Stein’s Anthropological Approach to the Humanities
Authors: Janik Piotr 
Published: February 2024

Should we approach the lived experience and/or understand the other with the sense of things? If this is the case, how are we to treat the human experience par excellence? Paradoxically, Edith Stein gives a fresh meaning to the Husserlian term “leiblicheSelbstgegebenheit.” In contrast to Max Scheler’s account, she develops the “persönliche Note” criterion of authenticity. And against Maetin Heidegger’s existential philosophy, a concern for human existence itself resonates. Based on these three dimensions, this article discusses the idea of “the lived experience” according to Edith Stein, that is, the human experience contemplated by the humanities.
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Received: 03/02/2023. Reviewed: 16/03/2023. Accepted: 30/03/2023.
Therapeutic Potential of Iron Chelators in Viral Diseases: A
Systematic Review
Authors: Lori Maryam Shamseddini , Khandani Azade Kalantari , Dehghannoudeh Gholamreza , Ohadi Mandana , Ansari Mehdi 
Published: February 2024

Background:
Iron chelators (ICs) have recently emerged as one of the new
methods of treatment for viral infections. This study aimed to evaluate the efficiency
and safety of natural ICs compared to synthetic ICs. Natural and synthetic ICs are the
most common therapeutic agents tested for the treatment of viral infections. When evaluated
against synthetic ICs, natural ICs are probably favored owing to their lower toxicity
and safer properties. The main objective of the present systematic review was to assess
the current evidence on the role of pharmacological mechanisms in the treatment of viral
infections.

Methods:
This study was designed as a systematic review in which search strategies
were focused on two electronic databases, PubMed, and Scopus, between 2017 and
2021. A search filter with two subjects, “iron chelators” and “viral infection”, was employed.

Results:
According to the results, both natural and synthetic chelators had a considerable
impact on the treatment of viral infections via various mechanisms, with natural
ICs being the most extensively used.

Conclusion:
Natural and synthetic ICs exert their effects through different pharmacological
mechanisms. Among these compounds, natural chelators are more widely used due
to their safety, efficacy, and a wider range of applications.
Long Non-coding RNA DLEU1 Promotes Progression of Osteoarthritis via
miR-492/TLR8 Axis
Authors: Ni Chenzhe , Zhang Wanglin , Qiu Sai , Cheng Hao , Ma Chunhui 
Published: February 2024

Background:
Long non-coding RNAs (LncRNAs) are generally reported to participate in the development of Osteoarthritis (OA) by acting as competing endogenous RNAs
(ceRNAs). However, the molecular mechanism is largely unknown. This study aimed to investigate the possible mechanisms contributing to osteoarthritis (OA).

Methods:
Four gene expression profiles from patients with OA were downloaded from a public
database and integrated to screen important RNAs associated with OA. Differentially expressed
(DE) lncRNAs, microRNAs (miRNAs), and mRNAs were filtered, and a ceRNA network was
constructed. An in vitro OA model was established by treating chondrocytes with IL-1β. The
expression levels of MMP-13, COL2A1, aggrecan, and RUNX2 were detected by qRT-PCR and
western blot. Cell proliferation ability was detected by CCK-8 assay. Flow cytometry was used
for apoptosis assay. A dual luciferase reporter gene was used to confirm the relationship between
DLEU1, miR-492, and TLR8

Results:
An OA-related ceRNA network, including 11 pathways, 3 miRNAs, 7 lncRNAs, and 16
mRNAs, was constructed. DLEU1 and TLR8 were upregulated, and miR-492 was downregulated
in IL-1β-induced chondrocytes. Overexpression of DLEU1 suppressed viability and promoted
apoptosis and extracellular matrix (ECM) degradation in IL-1β induced chondrocytes. Luciferase
reporter assay validated the regulatory relations among DLEU1, miR-492, and TLR8. Further
study revealed that the effects of DLEU1 on chondrocytes could be reversed by miR-492.

Conclusion:
DLEU1 may be responsible for the viability, apoptosis, and ECM degradation in
OA via miR-492/TLR8 axis
AEG-1 as a Novel Therapeutic Target in Colon Cancer: A Study from
Silencing AEG-1 in BALB/c Mice to Large Data Analysis
Authors: Sriramulu Sushmitha , Malayaperumal Sarubala , Banerjee Antara , Anbalagan Muralidharan , Kumar Makalakshmi Murali , Radha Rajesh Kanna Nandagopal , Liu Xingyi , Zhang Hong , Hu Guang , Sun Xiao-Feng , Pathak Surajit 
Published: February 2024

Background:
Astrocyte elevated gene-1 (AEG-1) is overexpressed in various malignancies. Exostosin-1 (EXT-1), a tumor suppressor, is an intermediate for malignant tumors. Understanding the mechanism behind the interaction between AEG-1 and EXT-1 may provide insights
into colon cancer metastasis.

Methods:
AOM/DSS was used to induce tumor in BALB/c mice. Using an in vivo-jetPEI transfection reagent, transient transfection of AEG-1 and EXT-1 siRNAs were achieved. Histological scoring, immunohistochemical staining, and gene expression studies were performed from excised tissues. Data from the Cancer Genomic Atlas and GEO databases were obtained to identify the expression status of AEG-1 and itsassociation with the survival.

Results:
In BALB/c mice, the AOM+DSS treated mice developed necrotic, inflammatory and dysplastic changes in the colon with definite clinical symptoms such as loss of goblet cells, colon
shortening, and collagen deposition. Administration of AEG-1 siRNA resulted in a substantial decrease in the disease activity index. Mice treated with EXT-1 siRNA showed diffusely reduced goblet cells. In vivo investigations revealed that PTCH-1 activity was influenced by upstream gene
AEG-1, which in turn may affect EXT-1 activity. Data from The Cancer Genomic Atlas and GEO
databases confirmed the upregulation of AEG-1 and downregulation of EXT-1 in cancer patients.

Conclusions:
This study revealed that AEG-1 silencing might alter EXT-1 expression indirectly
through PTCH-1, influencing cell-ECM interactions, and decreasing dysplastic changes, proliferation and invasion.
Efficacy and Safety of Bempedoic Acid in Patients with High Cardiovascular
Risk: An Update
Authors: Caklili Ozge Telci , Rizzo Manfredi , Cesur Mustafa 
Published: February 2024

:
Statins play a significant role in the prevention of cardiovascular (CV) diseases (CVDs);
however, non-adherence with statin treatment or statin intolerance (mainly attributed to muscleassociated
side effects) is not uncommon. New agents such as bempedoic acid (BA) can provide
more treatment options. BA is administered orally, once daily, at a dose of 180 mg in current clinical
practice. It can decrease circulating low-density lipoprotein cholesterol (LDL-C) levels by nearly
30% as monotherapy or by 20% as an add-on to statins. CV outcome studies have shown that BA
decreases major adverse CV event risk in patients with established CVD or high CV risk by 13%.
When patients with high CV risk were analyzed alone, the risk reduction was 30%. Its side effects
include a rise in serum uric acid levels and liver enzyme activity, whereas it does not increase diabetes
risk as statins do. BA can be used as adjunctive therapy to statins in patients at high CV risk in
whom lipid targets cannot be achieved or as an alternative to statins in patients with statin intolerance.
Sagittal Abdominal Diameter as the Best Predictor of Metabolic
Syndrome in HIV-Infected Men on Antiretroviral Therapy
Authors: Drvendžija Zorka , Galić Biljana Srdić , Vujanović Miloš , Marić Daniela , Jovanović Nina Brkić , Brkić Snežana , Turkulov Vesna , Ilić Dalibor 
Published: February 2024

Background:
The interaction of human immunodeficiency virus (HIV), host and antiretroviral
therapy (ART) causes a range of metabolic disorders that can be characterized as a
metabolic syndrome (MetS) that increases the cardiovascular risk. MetS involves central obesity,
which can be detected using different anthropometric parameters.

Objective:
To assess the abilities of different anthropometric parameters in the prediction of MetS
in HIV-infected men on ART.

Method:
The study involved 92 male participants (mean age 44.46±10.38 years), divided into two
groups: with and without MetS. All subjects underwent biochemical evaluation (triglycerides,
HDL-cholesterol, fasting glucose), blood pressure measurement and anthropometric assessment:
body mass, body height, body mass index (BMI), body fat mass, body circumferences (chest, upper
arm, forearm, waist, hip, proximal and middle thigh and calf), sagittal abdominal diameter
(SAD), skinfold thicknesses (subscapular, anterior and posterior upper arm, anterior and lateral
forearm, abdominal, supraspinal, thigh and calf), waist-to-hip ratio (WHR), waist-to-height ratio
(WHtR), waist-to-thigh ratio (WTR), sagittal abdominal diameter–to-body height ratio (SADH),
body adiposity index (BAI) and conicity index. MetS was specified according to IDF criteria.

Results:
Subjects with MetS had statistically significant higher values of all anthropometric parameters
except middle thigh circumference, calf skinfold and body height. According to ROC
analysis and Binary Logistic Regression, SAD has been shown as the best predictor of MetS with
a predictive value of 21.40 cm (AUC:0.91), followed by WHR with a predictive value of 0.93.

Conclusion:
Sagittal abdominal diameter is the strongest anthropometric indicator of MetS in
HIV-infected patients on ART.