bluuebunny/crossref_metadata_2025_split huggingface dataset:
PI3K/Akt signaling pathway mediates the effect of low-dose boron on barrier function, proliferation and apoptosis in rat intestinal epithelial cells
Authors: Chen Shuqin , Huang Jialiang , Liu Ting , Zhang Feng , Zhao Chunfang , Jin Erhui , Li Shenghe 
Published: January 2024

AbstractBoron is an essential trace element with roles in growth, development, and physiological functions; however, its mechanism of action is still unclear. In this study, the regulatory roles of the PI3K/Akt signaling pathway on boron-induced changes in barrier function, proliferation, and apoptosis in rat intestinal epithelial cells were evaluated. Occludin levels, the proportion of cells in the G2/M phase, cell proliferation rate, and mRNA and protein expression levels of PCNA were higher, while the proportions of cells in the G0/G1 and S phases, apoptosis rate, and caspase-3 mRNA and protein expression levels were lower in cells treated with 0.8 mmol/L boron than in control IEC-6 cells (P < 0.01 or P < 0.05). However, 40 mmol/L boron decreased ZO-1 and Occludin levels, the proportion of cells in the G2/M phase, cell proliferation rate, and mRNA and protein levels of PCNA and increased the apoptosis rate and caspase-3 mRNA expression (P < 0.01 or P < 0.05). After specifically blocking PI3K and Akt signals (using LY294002 and MK-2206 2HCL), 0.8 mmol/L boron had no effects on Occludin, PCNA level, apoptosis rates, and caspase-3 levels (P < 0.05); however, the proliferation rate and PCNA levels decreased significantly (P < 0.01 or P < 0.05). The addition of 40 mmol/L boron did not affect ZO-1 and Occludin levels and did not affect the apoptosis rate or PCNA and caspase-3 levels. These results suggested that the PI3K/Akt signaling pathway mediates the effects of low-dose boron on IEC-6 cells.
Bacteriophage-mediated decolonization of Klebsiella pneumoniae in a novel Galleria mellonella gut colonization model with Enterobacteriaceae
Authors: Mirza Kamran A. , Nietzsche Sandor , Makarewicz Oliwia , Pletz Mathias W. , Thieme Lara 
Published: January 2024

AbstractGalleria mellonella larvae have emerged as an invertebrate model for investigating bacterial pathogenesis and potential therapies, addressing ethical concerns related to mammalian models. This model has the advantage of having a simple gut microbiome, which is suitable for gut colonization studies. Intestinal colonization by Enterobacteriaceae significantly contributes to the spread of antibiotic resistance. This study aimed to establish a novel Enterobacteriaceae gut colonization larval model and assess its suitability for evaluating distinct antimicrobial efficacies. Larvae were force-fed sequentially with bacterial doses of K. pneumoniae and E. coli at 0, 24, and 48 h, with survival monitoring at 24 h intervals. Bacterial counts were assessed after 48 h and 120 h of force-feeding. Successfully colonized larvae were subjected to one-time force feeding of a bacteriophage cocktail (107 PFU/larvae) or MIC-based meropenem and ciprofloxacin. The colonized bacterial load was quantified by CFU count. Three doses of 106 CFU/larvae resulted in stable gut colonization, independent of the K. pneumoniae or E. coli strain. Compared with the control, force-feeding of the bacteriophage reduced the colonization of the strain Kp 419614 by 5 log10 CFU/larvae, while antibiotic treatment led to a 3 log10 CFU/larval reduction. This novel G. mellonella model provides a valuable alternative for gut colonization studies, facilitating proof-of-concept investigations and potentially reducing or replacing follow-up experiments in vertebrate models.
The Rogdi knockout mouse is a model for Kohlschütter–Tönz syndrome
Authors: Jimenez-Armijo Alexandra , Morkmued Supawich , Ahumada José Tomás , Kharouf Naji , de Feraudy Yvan , Gogl Gergo , Riet Fabrice , Niederreither Karen , Laporte Jocelyn , Birling Marie Christine , Selloum Mohammed , Herault Yann , Hernandez Magali , Bloch-Zupan Agnès 
Published: January 2024

AbstractKohlschütter–Tönz syndrome (KTS) is a rare autosomal recessive disorder characterized by severe intellectual disability, early-onset epileptic seizures, and amelogenesis imperfecta. Here, we present a novelRogdimutant mouse deleting exons 6–11- a mutation found in KTS patients disablingROGDIfunction. ThisRogdi−/−mutant model recapitulates most KTS symptoms. Mutants displayed pentylenetetrazol-induced seizures, confirming epilepsy susceptibility. Spontaneous locomotion and circadian activity tests demonstrateRogdimutant hyperactivity mirroring patient spasticity. Object recognition impairment indicates memory deficits.Rogdi−/−mutant enamel was markedly less mature. Scanning electron microscopy confirmed its hypomineralized/hypomature crystallization, as well as its low mineral content. Transcriptomic RNA sequencing of postnatal day 5 lower incisors showed downregulated enamel matrix proteinsEnam, Amelx,andAmbn. Enamel crystallization appears highly pH-dependent, cycling between an acidic and neutral pH during enamel maturation.Rogdi−/−teeth exhibit no signs of cyclic dental acidification. Additionally, expression changes inWdr72,Slc9a3r2, andAtp6v0cwere identified as potential contributors to these tooth acidification abnormalities. These proteins interact through the acidifying V-ATPase complex. Here, we present theRogdi−/−mutant as a novel model to partially decipher KTS pathophysiology.Rogdi−/−mutant defects in acidification might explain the unusual combination of enamel and rare neurological disease symptoms.
Synergism between coexisting eye diseases and sex in increasing the prevalence of the dry eye syndrome
Authors: Stang Andreas , Schmidt Börge , Schramm Sara , Kowall Bernd , Jöckel Karl-Heinz , Erbel Raimund , Kuss Oliver , Geerling Gerd 
Published: January 2024

AbstractThe aim was to investigate prevalence of dry eye syndrome (DES) in a population-based sample in Germany. The association between coexisting eye diseases and DES was also of interest. We recontacted participants of the Heinz Nixdorf Recall study between 2018 and 2021 by postal questionnaire that included the Women’s Health Study questionnaire on DES. We estimated prevalence of DES and examined DES-associated factors among 2095 participants aged 62–91 years. We performed interaction analyses between sex and coexisting eye diseases in relation to the DES prevalence and performed bias analyses to examine the robustness of the results. The DES prevalence was 31.5% (34–36% after correction for potential non-response bias, 24.1% after correction for outcome misclassification) and it was almost 2.1-times higher in women than in men (women 42.3%, men 20.4%). Among DES subjects, 70.3% had received treatment in the previous 12 months. There was synergism between female sex and coexisting eye diseases (cataract, glaucoma, macular degeneration) in terms of DES prevalence. The extrapolated numbers of patients aged 62–91 years with DES in Germany are 1.1–1.3 million men and 6.1–6.8 million women. The observed synergism may be explained by differences in ocular physiology, subjective perception and response behavior. Women with eye diseases (cataract, glaucoma, macula degeneration) appear to have a markedly higher susceptibility to suffer from DES than men, so that a diagnostic workup of DES symptoms is particularly justified in women with these eye diseases.
Social and political trust diverge during a crisis
Authors: Aassve Arnstein , Capezzone Tommaso , Cavalli Nicolo’ , Conzo Pierluigi , Peng Chen 
Published: January 2024

AbstractThis study shows that social and political trust may diverge in the face of shared threats, and that this pattern is driven by negative information about crisis management. Leveraging a three-wave panel survey and an information-provision experiment in the USA during the COVID-19 crisis, our research reveals that negative perceptions of pandemic management lead to a decline in political trust and a parallel increase in social trust. This dynamic is pronounced among government supporters, who, confronted with COVID-19 challenges, experience a substantial erosion of political trust. Simultaneously, there is a notable rise in social trust within this group. Our analysis suggests that, as government supporters attributed more responsibility for the crisis to their political leader, political trust was supplanted by social trust. Disenchanted voters, feeling let down by institutions, sought support in society. Both the survey and the experiment underscore that societal shocks can prompt individuals to shift from relying on formal institutions to informal ones as a coping strategy. This research contributes a generalizable framework explaining how negative perceptions of crisis management can lead societies to substitute political trust with social trust, advancing our understanding of societal responses to shared threats and adaptive strategies during crises.
RETRACTED ARTICLE: A vehicular network based
intelligent transport system for smart cities using machine learning
algorithms
Authors: Prakash J. , Murali L. , Manikandan N. , Nagaprasad N. , Ramaswamy Krishnaraj 
Published: January 2024

AbstractSmart cities and the Internet of Things have enabled the integration of
communicating devices for efficient decision-making. Notably, traffic congestion is one
major problem faced by daily commuters in urban cities. In developed countries,
specialized sensors are deployed to gather traffic information to predict traffic
patterns. Any traffic updates are shared with the commuters via the Internet. Such
solutions become impracticable when physical infrastructure and Internet connectivity
are either non-existent or very limited. In case of developing countries, no roadside
units are available and Internet connectivity is still an issue in remote areas.
Internet traffic analysis is a thriving field of study due to the myriad ways in which
it may be put to practical use. In the intelligent Internet-of-Vehicles (IOVs), traffic
congestion can be predicted and identified using cutting-edge technologies. Using
tree-based decision-tree, random-forest, extra-tree, and XGBoost machine learning (ML)
strategies, this research proposes an intelligent-transport-system for the IOVs-based
vehicular network traffic in a smart city set-up. The suggested system uses ensemble
learning and averages the selection of crucial features to give high detection accuracy
at minimal computational costs, as demonstrated by the simulation results. For IOV-based
vehicular network traffic, the tree-based ML approaches with feature-selection (FS)
outperformed those without FS. When contrasted to the lowest KNN accuracy of 96.6% and
the highest SVM accuracy of 98.01%, the Stacking approach demonstrates superior accuracy
as 99.05%.
Suspension culture of stem cells established of Calendula officinalis L.
Authors: Kaňuková Šarlota , Lenkavská Klaudia , Gubišová Marcela , Kraic Ján 
Published: January 2024

AbstractPlant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or stem cell-like cells in other organs and tissues of the plant body, as well as the possibility of de novo generation of meristematic cells from differentiated cells, allow to consider the establishment of stem cell cultures in a broader range of species. This study aimed to establish a stem cell culture of the medicinal plant Calendula officinalis L. Callus tissues were induced from leaf and root explants, and already at this stage, stem and dedifferentiated cells could be identified. The cell suspension cultures established both from the root- and leaf-derived calli contained a high proportion of stem cells (92–93% and 72–73%, respectively). The most effective combination of growth regulators for the development of stem cells in calli as well as cell cultures was 1.0 mg/L 2,4-D and 0.5 mg/L BAP. The highest amount of stem cells (5.60–5.72 × 105) was in cell suspension derived from the roots. An effective protocol for the establishment of marigold stem cell suspension culture was developed. The ratio of root-derived stem cells against dedifferentiated cells exceeded 90%.
The greening-causing agent alters the behavioral and electrophysiological responses of the Asian citrus psyllid to a putative sex pheromone
Authors: Volpe Haroldo X. L. , Carmo-Sousa Michele , Luvizotto Rejane A. G. , de Freitas Renato , Esperança Victoria , Darolt Josiane C. , Pegoraro Abner A. L. , Magalhães Diego M. , Favaris Arodi P. , Wulff Nelson A. , Miranda Marcelo P. , Bento José Maurício S. , Leal Walter S. 
Published: January 2024

AbstractThe Asian Citrus Psyllid (ACP), Diaphorina citri, is a vector of the pathological bacterium Candidatus Liberibacter asiaticus (CLas), which causes the most devastating disease to the citrus industry worldwide, known as greening or huanglongbing (HLB). Earlier field tests with an acetic acid-based lure in greening-free, ‘Valencia’ citrus orange groves in California showed promising results. The same type of lures tested in São Paulo, Brazil, showed unsettling results. During the unsuccessful trials, we noticed a relatively large proportion of females in the field, ultimately leading us to test field-collected males and females for Wolbachia and CLas. The results showed high rates of Wolbachia and CLas infection in field populations. We then compared the olfactory responses of laboratory-raised, CLas-free, and CLas-infected males to acetic acid. As previously reported, CLas-uninfected males responded to acetic acid at 1 µg. Surprisingly, CLas-infected males required 50 × higher doses of the putative sex pheromone, thus explaining the failure to capture CLas-infected males in the field. CLas infection was also manifested in electrophysiological responses. Electroantennogram responses from CLas-infected ACP males were significantly higher than those obtained with uninfected males. To the best of our knowledge, this is the first report of a pathogen infection affecting a vector’s response to a sex attractant.
Industrial camera model positioned on an effector for automated tool center point calibration
Authors: Konecny Jaromir , Beremlijski Petr , Bailova Michaela , Machacek Zdenek , Koziorek Jiri , Prauzek Michal 
Published: January 2024

AbstractThe study presents a novel, full model of an industrial camera suitable for robotic manipulator tool center point (TCP) calibration. The authors propose a new solution which employs a full camera model positioned on the effector of an industrial robotic arm. The proposed full camera model simulates the capture of a calibration pattern for use in automated TCP calibration. The study describes an experimental test robot stand for producing a reference data set, a full camera model, the parameters of a generally known camera obscura model, and a comparison of proposed solution with the camera obscura model. The results are discussed in the context of an innovative approach which features a full camera model to assist the TCP calibration process. The results showed that the full camera model produced greater accuracy, a significant benefit not provided by other state-of-the-art methods. In several cases, the absolute error produced was up to seven times lower than with the state-of-the-art camera obscura model. The error for small rotation (max. of 5$$^\circ $$

∘

) and small translation (max. of 20 mm) was 3.65 pixels. The results also highlighted the applicability of the proposed solution in real-life industrial processes.
Combined use of anticoagulant and antiplatelet on outcome after stroke in patients with nonvalvular atrial fibrillation and systemic atherosclerosis
Authors: Heo JoonNyung , Lee Hyungwoo , Lee Il Hyung , Lim In Hwan , Hong Soon-Ho , Shin Joonggyeong , Nam Hyo Suk , Kim Young Dae 
Published: January 2024

AbstractThis study aimed to investigate whether there was a difference in one-year outcome after stroke between patients treated with antiplatelet and anticoagulation (OAC + antiplatelet) and those with anticoagulation only (OAC), when comorbid atherosclerotic disease was present with non-valvular atrial fibrillation (NVAF). This was a retrospective study using a prospective cohort of consecutive patients with ischemic stroke. Patients with NVAF and comorbid atherosclerotic disease were assigned to the OAC + antiplatelet or OAC group based on discharge medication. All-cause mortality, recurrent ischemic stroke, hemorrhagic stroke, myocardial infarction, and bleeding events within 1 year after the index stroke were compared. Of the 445 patients included in this study, 149 (33.5%) were treated with OAC + antiplatelet. There were no significant differences in all outcomes between groups. After inverse probability of treatment weighting, OAC + antiplatelet was associated with a lower risk of all-cause mortality (hazard ratio 0.48; 95% confidence interval 0.23–0.98; P = 0.045) and myocardial infarction (0% vs. 3.0%, P < 0.001). The risk of hemorrhagic stroke was not significantly different (P = 0.123). OAC + antiplatelet was associated with a decreased risk of all-cause mortality and myocardial infarction but an increased risk of ischemic stroke among patients with NVAF and systemic atherosclerotic diseases.
Two new species of Tasiocera Skuse from Baltic amber (Diptera, Limoniidae)
Authors: Kania-Kłosok Iwona , Wąsacz Patryk , Tuchowski Michał , Krzemiński Wiesław 
Published: January 2024

AbstractThe paper presents very rare fossil record of crane flies. Inclusions in Baltic amber have documented the second evidence of the existence of the genus Tasiocera (Diptera: Limoniidae: Chioneinae) in ancient epochs of Earth’s history. Hypotheses were also introduced regarding the environmental preferences of fossil Dasymolopilus in relation to modern representatives of the subgenus. The genus is numerous in species and widespread in the modern fauna, while only one species, Tasiocera (Dasymolophilus) circumcincta, has been known from the fossil record to date. The paper presents an additional description and graphic documentation of this species based on new materials. The discovery of two other species Tasiocera (Dasymolophilus) gorskii sp. nov. and Tasiocera (Dasymolophilus) baltica sp. nov. in Eocene resins documents the occurence of Tasiocera since Eocene. Interestingly, only subgenus Dasymolophilus is represented both in extinct and extant fauna while subgenus Tasiocera is not known from the fossil record. The new discovery is very significant, and the result of the study adds a brick to our understanding of the evolution of this group of insects and their habitat.
Synthesis of colloidal silica nanofluid and assessment of its impact on interfacial tension (IFT) and wettability for enhanced oil recovery (EOR)
Authors: Mansouri Zadeh Morteza , Amiri Fatemeh , Hosseni Seyednooroldin , Ghamarpoor Reza 
Published: January 2024

AbstractEver-increasing global energy demand, from one hand and reduced oil initially in place in oil reservoirs due to production and reduced natural reservoir production capacity, on the other hand, has encouraged researchers to investigate different methods to improve and increase enhanced oil recovery (EOR) from oil reservoirs. One method is to employ nanotechnology in injected water, where nanoparticles could affect interfacial tension (IFT) between water and oil and wettability through properties, including high specific surface area and nanoparticle size. However, a major challenge in using nanoparticles in injected water is the instability of these particles in water, which ultimately reduces the efficiency of EOR. These particles cannot be stabilized through conventional methods at a large scale. In this study, stabilized silica nanoparticles were synthesized in the water phase using sodium silicate and sol–gel processes. The stability of this nanofluid was studied in seawater, and then its effect on IFT and changing wettability was examined. According to the results, seawater containing 40 times diluted nanofluid could obtain 41% reduced IFT and 40% alteration in wettability of carbonate core becoming more water-wet and ultimately 13.7% improved final oil recovery in secondary oil recovery and 8.3% improved final oil recovery in third EOR.
In vitro feeding of all life stages of two-host Hyalomma excavatum and Hyalomma scupense and three-host Hyalomma dromedarii ticks
Authors: Elati Khawla , Benyedem Hayet , Fukatsu Kohsuke , Hoffmann-Köhler Peggy , Mhadhbi Moez , Bakırcı Serkan , Bilgiç Hüseyin Bilgin , Karagenç Tülin , Darghouth Mohamed Aziz , Nijhof Ard M. 
Published: January 2024

AbstractTicks are blood-sucking ectoparasites and can transmit various pathogens of medical and veterinary relevance. The life cycle of ticks can be completed under laboratory conditions on experimental animals, but the artificial feeding of ticks has attracted increased interest as an alternative method. This study represents the first report on the successful in vitro feeding of all life stages of two-host tick species, Hyalomma scupense and Hyalomma excavatum, and the three-host tick Hyalomma dromedarii. The attachment and engorgement rates of adults were 84% (21/25) and 76% (19/25) for H. scupense females. For adult H. excavatum and H. dromedarii, 70% (21/30) and 34.4% (11/32) of the females attached and all attached females successfully fed to repletion. The oviposition rates of the artificially fed females were 36.4%, 57.1% and 63.1% for H. dromedarii, H. excavatum and H. scupense, respectively, with a reproductive efficiency index varying between 44.3 and 60.7%. For the larvae, the attachment and engorgement rates were 44.2% (313/708) and 42.8% (303/708) for H. dromedarii, 70.5% (129/183) and 56.8% (104/183) for H. excavatum and 92.6% (113/122) and 55.7% (68/122) for H. scupense. The attachment and engorgement rates for the nymphs were 90.2% (129/143) and 47.6% (68/143) for H. dromedarii, 66.7% (34/51) and 41.2% (21/51) for H. excavatum, and 44.1% (30/68) and 36.8% (25/68) for H. scupense. Molting rates of the immature stages varied between 71.3% (216/303) and 100% (68/68) for the larvae and between 61.9% (13/21) and 96% (24/25) for the nymphs. The successful in vitro feeding of all stages of the three Hyalomma species makes this method a valuable tool for tick research, with potential applications in studies on the pathogens transmitted by these tick species such as Theileria annulata.