bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streamlined Biotinylation, Enrichment and Analysis for Enhanced Plasma Membrane Protein Identification Using Turboid and Turboid-Start Biotin Ligases
Authors: SARIHAN MEHMET , KASAP MURAT , AKPINAR GURLER 
Published: November 2023

Abstract
Plasma membrane proteins play pivotal roles in various cellular events and are crucial in disease pathogenesis, making their comprehensive characterization vital for biomedical research. However, the hydrophobic nature and low expression levels of PMPs pose challenges for conventional enrichment methods, hindering their identification and functional profiling. In this study, we presented a novel TurboID-based enrichment approach for PMPs that helped overcoming some of the existing limitations. We evaluated the efficacy of TurboID and its modified form, TurboID-START, in PMP enrichment, achieving more efficient and targeted labeling of PMPs without the need for stable cell line generation. This approach significantly reduced non-specific biotinylation events, leading to improved PMP enrichment and enabling a precise assessment of the subcellular proteome associated with the plasma membrane. Our findings highlighted the dynamic nature of the plasma membrane as well and captured transient associations of proteins with the plasma membrane. The novel TurboID-based enrichment approach presented here offers promising prospects for in-depth investigations into PMPs and their roles in cellular processes.
Metal-metal attachment of absorbers for Gamma-Ray TES
Authors: Dasgupta Adrika , Becker Daniel , Bennett Douglas , Keller Mark , Kislat Fabian , Schmidt Daniel , Swetz Daniel , Weber Joel , Wimalasena Kasun 
Published: November 2023

Abstract

In this work, we are reporting on the development of a method to attach Sn absorbers to the Transition Edge Sensors (TESs) with BiSn spheres (metal-to-metal attachment), using a die bonder, with the aim of improving thermalization, potentially reducing the presence of an a-thermal component in the tails of signal pulses. We describe our efforts towards finding optimal temperature and pressure conditions for making this contact and the progress toward contact resistance measurements of these joints.
The correlation between levels of serum neurofilaments light and cognitive performance in older adults within the United States
Authors: Xue Kun , Xing Shanshan 
Published: November 2023

Abstract
INTRODUCTION:
The aim of this study was to ascertain the association between sNfL levels and cognitive function.
METHODS:
In order to perform our analysis, we used National Health and Nutrition Examination Survey (NHANES) data from 2013 to 2014. 502 elderly persons (older than 60) were the subject of our investigation. Using a variety of measures, including the CERAD Word List Learning Test (WLLT), Word List Recall Test (WLRT), Intrusion Word Count measures (WLLT-IC and WLRT-IC), Animal Fluency Test (AFT), and Digit Symbol Substitution Test (DSST), the main goal was to assess cognitive performance. We utilized multiple linear regression and cubic spline analyses, correcting for a number of factors.
RESULTS:
The inverse association between sNfL and DSST was found to be statistically significant (β: -2.3, p = .045), indicating a detrimental impact on the DSST score. There was no indication that this connection had a plateau effect.
CONCLUSIONS:
There was a notable inverse correlation observed between sNfL levels and processing speed, sustained attention, and working memory in older adults aged 60 years and above.
Co-infection of Solanum tuberosum with PVX and PVY leads to systemic virus-mediated gene expression
Authors: Mehdi Rabih , Eydam Julia , Sonnewald Uwe 
Published: November 2023

Abstract
Background
Breeding novel crop cultivars can be an extremely laborious and time-consuming process, especially when it comes to highly heterozygous and tetraploid potato (Solanum tuberosum L.). Establishing biotechnological techniques to understand gene functions and to accelerate breeding processes are therefore inevitable to sustain resilient potato cultivars for future food security. Apart from being pathogens, viruses represent suitable vectors for delivering genetic information into plant cells. Taking regulatory obstacles for genetically modified crops into account, RNA viruses provide a convenient tool to introduce desired RNA molecules into plants without the application of foreign DNA. Hence, developing an RNA virus-mediated gene expression system to study and improve crop plants is of great importance. Additionally, plant RNA viruses represent an opportunity to obtain modified crops without the necessity for tissue culture. With this work, we seek to establish a virus-mediated gene expression system for cultivated potato.
Results
Irrespective of reports claiming successful gene expression or silencing upon infection of potato (S. tuberosum) with RNA viruses, such as potato virus X (PVX), single Agrobacterium-mediated infections with PVX did not lead to a sufficient systemic infection and concomitant expression of a green fluorescent protein (GFP) in various potato cultivars. Using the tuber-bearing species Solanum pinnatisectum for infections, systemic movement of PVX-GFP was detectable throughout the diploid wild potato, pointing out limitations for application in tetraploid potato cultivars. Further, hetero-graftings with Nicotiana benthamiana and S. tuberosum indicated that PVX-GFP can move through the vasculature of potato without leaving the transport tissue. Nonetheless, coinfection of PVX-GFP and potato virus Y (PVY) enabled adequate systemic spreading accompanied by GFP expression in different potato cultivars.
Conclusion
PVX-mediated gene expression is limited in cultivated potato. Utilizing PVY for co-infections facilitates systemic PVX spreading and accompanying expression of a desired gene. This method allows fast and uncomplicated viral delivery of genetic information into S. tuberosum opening up opportunities not only to express desired proteins but also to silence genes of interest and thereby establish epigenetic modifications serving as tool for basic science and possibly for plant breeding purposes.
Effect of Educational Campaign on Interpersonal Communication Skills among Health Care Professionals: Pre-post study, Jeddah, KSA
Authors: Mahmoud Manal Ibrahim Hanafi , Badawey Jude Tareq , Kouki Ahmad Mohamed Khaled , Malik Louay Fadi , Shakally Salman Mohamed Samih , Nasser Asmaa Abdel 
Published: November 2023

Abstract
Background
Communication among healthcare team members is crucial for effective patient care. It helps ensure that everyone involved in the healthcare process is aware of the patient’s needs and treatment plan, which increases the likelihood of positive outcomes and reduces the risk of medical errors. Strong communication can help build trust and collaboration among healthcare team members, which can improve job satisfaction and lead to better patient outcomes.
Aim
To study and explore the effect of an educational campaign on interpersonal communication skills among healthcare professionals in Jeddah, KSA.
Methods
A quasi-experimental pre- and post- study was carried out on a convenient sample of 150 healthcare professionals. A specially prepared self-administered, pre-coded, closed-ended, pilot-tested, reliable questionnaire format was used. Self- assessment communication skills inventory questionnaires were completed initially in the pre-study phase and at the end of a specifically arranged educational campaign as the post-study phase. To control for potential confounding factors, multivariate analyses using a forward stepwise (Wald) method were carried out to determine independent predictors of interpersonal communication skills among the healthcare professionals.
Results
There was a significant improvement of the measured responses to the interpersonal communication skill inventory in all its domains, namely: sending a clear message (p = 0.000), listening (p = 0.000), giving and receiving feedback (p = 0.000) and handling emotional reactions (p = 0.000). Significant independent predictors of interpersonal communication skills were nationality, mother language, specialty, qualification, daily working hours, number of monthly shifts and perceived satisfaction with the work environment. The mean evaluation of the whole campaign out of 10 was 9.6 (4.85), with a mean percent score for evaluation of 77.6 (22.15).
Conclusion
these educational campaigns for the development of interpersonal communication skills have desirable effects on healthcare professionals’ communication abilities and capabilities. Healthcare organizations should invest in training programs that develop healthcare professionals’ communication skills to improve healthcare quality and patient safety.
The Expression of VDBP in the Severe COVID-19 and its Role in Acute Lung Injury
Authors: Jiang Hongjuan , Chi Xiangyu , sun Yanhong , Wang Jing , Li Hongwen 
Published: November 2023

Abstract
Background
Vitamin D-binding protein (VDBP) may be involved in the occurrence and development of COVID-19. This study intends to investigate the expression of VDBP in COVID-19 and to explore the role of VDBP through animal and cellular models of acute lung injury (ALI).
Methods
The serum of COVID-19 and common pneumonia over 65 years old in the Department of Geriatrics of the Shandong Provincial Hospital Affiliated to Shandong First Medical University were collected from 1 December 2022 to 30 July 2023. ELISA was used to detect the expression of serum VDBP. The mouse model of ALI was established by intraperitoneal injection of LPS, and the expression of VDBP in the lung tissue of ALI mice was detected by immunohistochemistry and Western blot. Construct a VDBP gene-silencing plasmid and transfect it into human alveolar epithelial A549 cells. After 72 hours of LPS intervention, collect cells and cell supernatant for testing. CCK8 detection of cell proliferation. Flow cytometry was used to detect apoptosis in cells.
Results
The VDBP of severe COVID-19 was higher than that of non-severe COVID-19 and common pneumonia (p < 0.05), but the VDBP was not an independent risk factor for the severity of COVID-19 (p > 0.05). Immunohistochemistry and Western blot suggest that VDBP in lung tissue of ALI mice was significant overexpression (p < 0.05). VDBP in LPS-induced injury of alveolar epithelial cells was significant overexpression (p < 0.05). The ELISA results showed that after LPS intervention, the inflammatory factor IL-1β and TNF-a was significantly reduced in the VDBP gene silencing group compared to the control group (p < 0.05). The cell proliferation ability of the VDBP gene silencing group was significantly increased compared to the control group, and the cell apoptosis rate was significantly reduced compared to the control group (p < 0.05).
Conclusion
The level of VDBP in severe COVID-19 was significantly increased. VDBP may play an inflammatory factor in ALI and promote apoptosis of bronchial epithelial cells.
The effect of asymmetric regulation on market competition: the case of the 2013 Telecomm Reform in Mexico
Authors: Silva Rodrigo Alcázar , Pastrana Julio Alberto Ramos 
Published: November 2023

Abstract

We examine the economic effects of the 2013 Telecommunications Reform in Mexico that implemented asymmetric regulation on the largest telecommunications operator in the country. This regulation imposed on the “preponderant” telecommunications operator, defined as the operator with more than 50% of the connections of all the sector, a series of requirements aimed to increase market competition and facilitate entry in the sector. We investigate the effects of this regulation on market competition using quarterly data from the Mexican telecommunications operators in the period 2010.3-2017.3. Results provide evidence that the asymmetric regulations fostered competition in two ways: they fostered entry into the sector, which resulted in a decrease of the market share of the preponderant agent, driven by a decrease in the market share of prepaid lines; and at the same time intensified competition by increasing the quality of the services provided by the preponderant agent, which resulted in a net gain in the number of lines transferred to this agent, and an increase in the number of postpaid lines.
Effects of Glaucoma Treatment on Ocular Surface and Tear Functions: Comparison of Trabeculectomy and Antiglaucomatous Drops
Authors: Mermer Abdussamet , Dursun Özer , Bucak Öznur , Sayar Hamide , Bektaş Fatma Merve , Eröz Pınar , Yılmaz Ayça 
Published: November 2023

Abstract
Aim
To investigate the tear function and ocular surface disease findings in glaucoma patients who received antiglaucomatous medication in one eye and trabeculectomy surgery in the other eye.
Methods
The patient group included 38 eyes of 19 patients who underwent trabeculectomy surgery with mitomycin C treatment in one eye at least 6 months ago and were followed up without medication, while the other eye was receiving antiglaucomatous medication. As a control group, 20 eyes of 20 healthy individuals were included in the study. Demographic data, follow-up period after trabeculectomy, antiglaucomatous medications, number of drops per day and duration of medication were recorded. Tear break-up time, corneal and conjunctival fluorescein staining, Schirmer II test and conjunctival impression cytology were performed.
Results
There was a statistically significant difference in tear break-up time, corneal and conjunctival fluorescein staining, Schirmer II test and Nelson staging levels in both eyes of glaucoma patients when compared with the control group (p0.05). Although not statistically significant, tear break-up time, Schirmer II test, punctate staining and Nelson staging results were found to be better with increasing postoperative time.
Conclusions
Medications used in glaucoma patients and trabeculectomy surgery with MMC induced OSD of similar severity in our patient group. There was no superiority between trabeculectomy with MMC and trabeculectomy with MMC in terms of OSD incidence.
(non-aspirin) NSAIDs use and risk of cardiovascular diseases: A Mendelian randomization study
Authors: Liu Guihong , Chen Tao , Zhang Xin , Hu Binbin , Shi Huashan 
Published: November 2023

Abstract
Objectives Non-steroidal anti-inflammatory drugs (NSAIDs) are currently the most common anti-inflammatory and analgesic drugs. Some clinical studies have reported that NSAIDs increase the incidence of several cardiovascular diseases (CVDs). However, a solid causal association has not been demonstrated. In this Mendelian randomization (MR) study, we investigated the causal association of NSAIDs use with the risk of CVDs.Methods A two-sample MR was utilized to determine whether there is a causal relationship between NSAIDs use and the risk of CVDs. Single-nucleotide polymorphisms(SNPs)associated with NSAIDs indices were used as instrumental variables to estimate the associations with the risk of CVDs. The dataset was obtained from genome-wide association studies (GWAS). Estimation of the causal effect was mainly performed using the random effects inverse-variance weighted method (IVW). Furthermore, Cochran’s Q test, MR-Egger intercept tests, MR-PRESSO, leave-one-out analyses, and funnel plot were used in the sensitivity analysis.Results NSAIDs use increase causally the risks of coronary heart disease [CHD; odds ratio(OR) = 1.005, 95% confidence interval(CI): 1.002–1.007, P < 0.05], heart failure(HF; OR = 1.091; 95% CI, 1.027–1.160; P < 0.05), atrial fibrillation(AF; OR = 1.087; 95% CI, 1.034–1.142; P < 0.05) with multiplicative random-effects IVW MR analysis. However, there was no suggestive evidence of a causal association between NSAIDs use and myocardial infarction (MI; OR = 1.001; 95% CI, 0.999–1.003; P = 0.23), or hypertension(OR = 1.001; 95% CI, 0.995–1.007; P = 0.72). The main results are kept stable in the sensitivity analysis.Conclusion This MR study provided support for a causal association of NSAIDs use with CHD, HF, and AF. However, it did not support an association of genetically predicted NSAIDs use on MI, and hypertension.
Evaluation of Case Discussing Teaching by Multidisciplinary team combined with Case-Based Learning Approach in Gynecological Oncology
Authors: Zhou Bo , Zhao Mengya , Yuan Yunhe , Hu Zheng 
Published: November 2023

Abstract
Background: In gynecologic oncology, traditional teaching methods have been challenged due to the complex disease nature and the uniqueness of the patient. To address these challenges, innovative approaches are needed.
Methods: This study aims to assess the effectiveness of the multi-disciplinary team (MDT) combined with case-based learning (CBL) teaching methods in gynecological oncology education for standardized resident trainees. The study includes second and third-year resident trainees who underwent standardized training in our department from 2021 to 2022. These trainees were randomly assigned to either an experimental group or a control group, each consisting of 15 individuals. The experimental group received instruction using the MDT+CBL combined teaching model, while the control group followed the traditional CBL teaching model. We compared post-teaching theoretical assessment scores and clinical case analysis abilities between the two groups. Furthermore, we assessed trainee satisfaction with teaching effectiveness through a questionnaire survey.
Results: The experimental group achieved significantly higher scores in both theoretical knowledge assessment and clinical case assessment compared to the control group (t = 5.681 and 5.388, respectively, P < 0.05). Additionally, the experimental group expressed significantly greater satisfaction with the teaching effectiveness in terms of stimulating learning interest, mastering basic theoretical knowledge and skills, engaging in self-directed learning through literature review, enhancing clinical reasoning abilities, and improving teamwork cooperation abilities. All of these aspects displayed statistical significance (t = 5.046, 5.512, 4.838, 4.580, 9.707, respectively, P < 0.05).
Conclusions:
The MDT+CBL teaching model significantly enhances teamwork skills among students, improves the quality of gynecological oncology education, kindles students' enthusiasm for learning, promotes self-directed learning through literature review, enhances the application of relevant theoretical knowledge, and provides a more comprehensive understanding of diseases. Consequently, it substantially elevates overall teaching quality and enhances students' clinical thinking effectiveness. This approach is not only valuable for obstetrics and gynecology (OB/GYN) but also offers a promising model for medical education across disciplines.
Navigating the Complexities of Constructions: Unveiling the Dichotomy Between Added-Values and Applications of Risk Management.
Authors: Alrabie Mohammad 
Published: November 2023

Abstract
The different processes, developments, and advancements associated with the modern trend of constructions are complicated, rapid, and involve great risks that could impact projects. Thus, the necessity for an overall risk management approach in constructions is on a constantly rising trajectory. The paper discusses the added-values of implementing risk management within the construction industry, as well as differentiating between the added-values of risk management, and risk management applications within the projects. The paper also discusses and evaluates the reliability of the added-values of risk management presented in the literature review against the analysis results from the participants. The analysis results showed a significant positive correlation between the participants’ years of experience and their awareness of risk management’s applications. The analysis also demonstrated the variation in significance in risk management applications among participants, which concluded that there is a fundamental difference between the added-values and the applications of risk management.
Chronic kidney disease as a risk factor for severe preoperative anemia and heart failure in elderly patients with hip fracture: a single-center retrospective cohort study
Authors: Meng Yao , Wang Zhiqian , Hou Zhiyong 
Published: November 2023

Abstract
Background
Patients with chronic kidney disease (CKD) are prone to hip fractures and have a worse preoperative prognosis than patients without CKD. However, few studies have examined this issue by directly comparing patients with and without CKD.
Methods
We conducted a retrospective analysis of hip fracture patients ≥ 65 years of age who attended a level 1 trauma centre in China from January 2017 to December 2020. The CKD and non-CKD groups were matched 1:1 using the propensity score matching (PSM) method. Demographic data, comorbidities and preoperative complications were collected from the hospital's electronic medical records for both groups of patients. Patients with preoperative heart failure and anemia were classified into two classes based on different B-type natriuretic peptide (BNP) and haemoglobin (Hgb) levels. The above data were compared between the two groups of patients. The association between CKD and preoperative heart failure and anemia was assessed using univariate and multivariate logistic regression and subgroup analysis.
Results
We finally included 126 patients with CKD and 126 patients without CKD. The incidence of both preoperative heart failure (66.7% vs. 47.6%) and anemia (61.4% vs. 38.6%) was higher in CKD patients than in non-CKD patients. Mild heart failure (BNP < 500pg/ml) and severe heart failure (BNP ≥ 500pg/ml), mild anemia (Hgb ≥ 9.0g/dL) and severe anemia (Hgb < 9.0g/dL) were statistically different between the two groups (P < 0.05). By logistic regression analysis, patients with CKD were risk factors for severe heart failure (OR, 2.773; 95% CI, 1.299–5.922) and severe anemia (OR, 4.702; 95% CI, 1.982–11.157). After subgroup analyses, the above conclusions remain valid.
Conclusion
Patients with CKD have a higher risk of severe heart failure and anemia preoperatively than non-CKD patients. Clinicians should strengthen preoperative management of such patients and make full preparations for the prevention of serious complications.
Zwint is highly expressed in ovarian cancer and promotes cell proliferation, migration and invasion
Authors: Liu Wen-ting , Chen Ling , Fu Fen , Wan Xian-xian , Zhang Meng-meng 
Published: November 2023

Abstract
Objective: To investigate the expression level of ZWINT in ovarian cancer and its impact on the proliferation, migration and invasion of ovarian cancer cells.
Methods: The differentially expressed genes in ovarian cancer tissues were identified from both the TCGA and GEO databases, followed by an analysis of their expression levels and investigation into their impact on patients' survival and prognosis; two ovarian cell models were established to interfere with gene expression, and the expression and interference effects were evaluated by RT-PCR and Western blot; cell proliferation was detected by plate cloning and Edu assay.
Result: ZWINT was identified as a differentially expressed gene in ovarian cancer through database analysis, and further comparison of gene expression profiles revealed its high expression levels in this malignancy; Kaplan-Meier analysis revealed a correlation between higher expression of ZWINT and worse survival prognosis in patients. The success of interfering with the ZWINT cell line was verified through RT-PCR and Western blot experiments. Plate cloning assay demonstrated a significant reduction in the number of cell colonies after ZWINT interference, while Edu assay indicated a decrease in the percentage of Edu-positive cells. Transwell assay suggested that cell migration and invasion were also affected by ZWINT interference.
Conclusion: ZWINT was highly expressed in ovarian cancer tissues, and could promote the proliferation, migration and invasion of cancer cells.