bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR/Cas9-induced double-strand breaks in huntingtin locus lead to CAG repeat contraction through the extensive DNA end resection and homology-mediated repair
Authors: Sledzinski Pawel , Nowaczyk Mateusz , Karwacka Marianna , Olejniczak Marta 
Published: November 2023

ABSTRACTExpansion of the CAG/CTG repeats in functionally unrelated genes is a causative factor in many inherited neurodegenerative disorders, including Huntington’s disease (HD), spinocerebellar ataxias (SCAs) and myotonic dystrophy type 1 (DM1). Despite many years of research, the mechanism responsible for repeat instability is unknown, and recent findings indicate the key role of DNA repair in this process. The repair of DSBs induced by genome editing tools results in the shortening of long CAG repeats in yeast models. Understanding this mechanism is the first step to developing a therapeutic strategy based on controlled shortening of repeats. The aim of this study was to characterize Cas9-induced DSB repair products in the endogenousHTTlocus in human cells and to identify factors affecting the formation of specific types of sequences. The location of the cleavage site and the surrounding sequence influence the outcome of DNA repair. DSBs within CAG repeats result in shortening of the repeats in frame in ∼90% of products. The mechanism of this contraction involves MRE11-CTIP and RAD51 activity and extensive DNA end resection reaching ∼5000 bp. We demonstrated that a DSB located upstream of CAG repeats induces polymerase theta-mediated end joining, resulting in deletion of the entire CAG tract. Furthermore, using unbiased proteomic analysis, we identified novel factors that may be involved in CAG sequence repair.Graphical abstractDNA double-strand break repair in the CAG repeat locus engages numerous factors from various repair pathways and depends mainly on DNA end resection, leading to tract shortening.
Running to remember: The effects of exercise on perineuronal nets, microglia, and hippocampal angiogenesis in female and male mice
Authors: Maheu Madeleine G. , James Noah , Clark Zach , Yang Alex , Patel Ridhi , Beaudette Shawn M. , MacPherson Rebecca E.K. , Duarte-Guterman Paula 
Published: November 2023

AbstractExercise is accepted as a positive health behaviour; however, the mechanisms of exercise on neuroprotection and cognitive health are not completely understood. The purpose of this study was to explore the neurobiological benefits of chronic treadmill exercise in female and male mice through its role in microglial content and morphology, cerebral vascularization, and perineuronal net (PNN) expression. We further examined how these neurobiological changes relate to spatial memory outcomes. Adult mice were assigned to a sedentary or treadmill exercise group for eight weeks. During the final week, all mice were trained on a spatial memory task (Barnes maze) and brains were collected for immunohistochemistry. Exercised mice made fewer errors than sedentary mice during the first two days of training and probe trial. Females, regardless of exercise training, made fewer errors during Barnes maze training and demonstrated a greater frequency of spatial strategy use compared to males. Exercised mice, regardless of sex, had fewer PNNs in the dentate gyrus of the hippocampus compared to sedentary controls. The number of PNNs in the dorsal dentate gyrus was positively correlated with total errors during training. During the probe, greater errors correlated with more PNNs among the exercised group only. Microglia count and cerebral vascularization were not affected by exercise, although proportions of microglia type (ameboid, stout/thick, and thick/thin) were regulated by exercise in the ventral dentate gyrus. We conclude that exercise decreases PNNs in the dentate gyrus in both sexes and this may be related to better spatial learning and memory.
Energy Landscape near Glioma Decipher the Role of Glioma Stem Cells within Glioma Hierarchy
Authors: Yao Mengchao , Su Yang , Xiong Ruiqi , Zhang Xile , Zhu Xiao-Mei , Chen Yong-Cong , Ao Ping 
Published: November 2023

AbstractGlioma stem cells (GSCs) have been recognized as key players in glioma recurrence and therapeutic resistance, presenting a promising target for novel treatments. However, the limited understanding of the role GSCs play in the glioma hierarchy has drawn controversy and hindered research translation into therapies. Despite significant advances in our understanding of gene regulatory networks, the dynamics of these networks and their implications for glioma remain elusive. This study employs a systemic theoretical perspective to integrate empirical knowledge into a core endogenous network model for glioma, thereby elucidating its energy landscape through network dynamics computation. The model identifies two stable states corresponding to astrocytoma and oligodendroglioma, connected by a transitional state associated with GSCs, indicating the instability of GSCsin vivoand providing negative proof of their role as the cellular origin for glioma. We also obtained various stable states further indicative of glioma’s multicellular origins and uncovered a group of transition states that could potentially induce tumor heterogeneity and therapeutic resistance. This research proposes that the transitional states linking both glioma stable states are central to glioma heterogeneity and therapy resistance, suggesting a combination of apoptosis-inducing and differentiation-promoting therapies as a potential strategy. Our approach holds promise for advancing glioma therapy by providing a new lens through which to view the complex landscape of glioma biology.
The identification of extensive samples of motor units in human muscles reveals diverse effects of neuromodulatory inputs on the rate coding
Authors: Avrillon Simon , Hug François , Enoka Roger , Caillet Arnault H. , Farina Dario 
Published: November 2023

AbstractMovements are performed by motoneurons transforming synaptic inputs into an activation signal that controls muscle force. The control signal emerges from interactions between ionotropic and neuromodulatory inputs to motoneurons. Critically, these interactions vary across motoneuron pools and differ between muscles. To provide the most comprehensive framework to date of motor unit activity during isometric contractions, we identified the firing activity of extensive samples of motor units in the Tibialis Anterior (129±44 per participant; n=8) and the Vastus Lateralis (130±63 per participant; n=8) muscles during isometric contractions of up to 80% of maximal force. From this unique dataset, the rate coding of each motor unit was characterised as the relation between its instantaneous firing rate and the applied force, with the assumption that the linear increase in isometric force reflects a proportional increase in the net synaptic excitatory inputs received by the motoneuron. This relation was characterised with a natural logarithm function that comprised two stages. The initial stage was marked by a steep acceleration of firing rate, which was greater for low- than medium- and high-threshold motor units. The second stage comprised a linear increase in firing rate, which was greater for high- than medium- and low-threshold motor units. Changes in firing rate were largely non-linear during the ramp-up and ramp-down phases of the task, but with significant prolonged firing activity only evident for medium-threshold motor units. Contrary to what is usually assumed, our results demonstrate that the firing rate of each motor unit can follow a large variety of trends with force across the pool. From a neural control perspective, these findings indicate how motor unit pools use gain control to transform inputs with limited bandwidths into an intended muscle force.
The Dynamics of Context-Dependent Space Representations in Auditory Cortex
Authors: Myoga Michael H. , Amaro Diana , Kello Valerija , Gumbert Matthias , Leibold Christian , Pecka Michael , Grothe Benedikt 
Published: November 2023

AbstractAuditory space is not mapped onto the receptor surface in the inner ear but results from complex neuronal computations by our brain. However, there is no consensus about the resulting neuronal representation of space in auditory cortex (AC) owing to contradicting reports: While many studies found a tuning preference for sound source positions in the contralateral hemisphere, others have observed an additional population of cells tuned to midline positions or even context-dependent dynamic changes in the tuning of individual cells. A fundamental difference across studies was the animals’ brain state, which might affect AC processing and thus the apparent nature of its spatial code. Yet no study to date investigated spatial tuning of identified AC neurons across brain states. Here, we employed longitudinal two-photon calcium imaging in the AC of mice under distinct states of wakefulness: anesthetized, idle awake, and during involvement in a go/no-go localization task. We find that previously reported differences in coding regimes are directly linked to wakefulness: A strong contralateral tuning bias is present under anesthesia, while pronounced and stable mid-line tuning appeared in awake but idle animals. Intriguingly, in localizing mice, tuning was different again with a large proportion of AC neurons responding to the position of the currently relevant sound-source. These population regimes remained stable across imaging sessions despite the spatial tuning of individual neurons being highly variable. Our findings resolve apparent contradictions in the literature and thus give a dynamical explanation for the multiple space representations in the AC.
COVID-19, destination image, and destination visit intention: unpacking the impacts of familiarity, generation, and gender amidst COVID-19
Authors: Hamdy Ahmed , Eid Riyad 
Published: November 2023

Purpose
This study aims to analyze the moderating roles of familiarity, generation and gender on the impacts of coronavirus fear-uncertainty on the destination image and visiting intentions post-COVID-19.

Design/methodology/approach
This paper seeks to provide evidence for a research conclusion by conducting a survey of 431 potential travelers of various nationalities who visited Egypt. The data were examined using structural equation modeling with a multigroup analysis and PROCESS MACRO.

Findings
The findings indicated that the links between coronavirus fear-uncertainty, the destination image and intention to visit were significantly different from one gender and generation group to another. Moreover, it showed that destination familiarity moderates the negative effects of coronavirus fear-uncertainty on the destination image and intention to visit.

Originality/value
To the best of the authors’ knowledge, this paper is the first to explore the moderating role of destination familiarity, generation and gender in the effects post-COVID-19 of coronavirus fear-uncertainty on the destination image and travelers’ intention to visit using generational cohort theory and gender schema theory.
Transcendence beyond sport: examining the dynamic power of sport human brand on national pride
Authors: Gulavani Susmit S. , Du James , James Jeffrey D. 
Published: November 2023

PurposeDrawing upon social judgment theory, the research examines whether changes in psychological involvement with a sport human brand owing to their sporting success can generate spillover effects on people's national pride, a proxy for the collective level of well-being and whether the individual's behavioral engagement in sport spectating will moderate this relationship.Design/methodology/approachLeveraging a quasi-natural experiment grounded in the 2021 Indian Premier League championship, the authors solicit responses from 296 representative individuals residing in India twice using a two-wave panel design. The authors conducted confirmatory factor analysis, repeated measures T-test, latent change score analysis, and structural regression analysis to examine the relationship between psychological involvement with sport human brand and national pride.FindingsThe results demonstrate a positive association between psychological involvement with sport human brand and national pride. Further, the findings indicate that an increase in psychological involvement with sport human brand was associated with an increase in national pride due to the successful athletic endeavor involving the sport human brand. However, the relationship between psychological involvement with sport human brand and national pride was invariant irrespective of patrons' spectatorship behavior.Originality/valueThis research demonstrates that sport human brands possess transformative soft power that extends their prerogative cultural identity personified by their athletic ability and success, allowing them to shape public sentiments of national pride via their profound influence through and beyond the complex network of brand ecosystems.
Preventive conservation: environmentally friendly sustainable current practices in Asian libraries
Authors: Rachman Yeni Budi , Ratnasari Wiwit , Tambunan Shuri Mariasih Gietty 
Published: November 2023

PurposeThis study aims to identify current practices of environmentally friendly sustainable preventive conservation in Asian libraries.Design/methodology/approachThis study uses a predominantly quantitative survey for data collection with a combination of open and closed questions. From 237 survey links sent, 22 responses were received from libraries in 16 Asian countries.FindingsThis study uncovers current practices in four areas of environmentally sustainable preventive conservation in Asia: collection preservation, environmental monitoring and management, integrated pest management, and collections care and handling. Challenges to implementing sustainable preventive conservation were also revealed.Research limitations/implicationsThe response rate was relatively low, and responses were not received from all countries in Asia. As such, while the study provides a starting point for further research, it cannot be assumed that the findings are representative of Asian libraries in general. In addition, some aspects of preventive conservation (for example, disaster preparedness, exhibition) and library preservation management were excluded from the study, and research on these topics, together with environmental and economic factors, is encouraged, along with qualitative research methods.Practical implicationsThis study highlights the environmentally friendly sustainable initiatives that libraries employ in their preservation programmes and increases awareness of challenges to implementing those initiatives.Originality/valueTo date there has been little research on environmentally sustainable preventive conservation practices within Asia's libraries. This study therefore makes a valuable contribution to understanding current practices of preventive conservation in Asia.
Leading well and staying psychologically healthy: the role of resources and constraints for managers in the healthcare sector
Authors: Gilbert Marie-Hélène , Dextras-Gauthier Julie , Boulet Maude , Auclair Isabelle , Dima Justine , Boucher Frédéric 
Published: November 2023

PurposeMaintaining a healthy and productive workforce is a challenge for most organizations. This is even truer for health organization, facing staff shortages and work overload. The aim of this study is to identify the resources and constraints that influence managers' mental health and better understand how they are affected by them.Design/methodology/approachA qualitative approach was chosen to document the resources, the constraints as well as their consequences on managers in their day-to-day realities. The sample included executive-, intermediate- and first-level managers from a Canadian healthcare facility. A total of 62 semi-structured interviews were conducted. The coding process was based on the IGLOO model of Nielsen et al. (2018) to which an employee-related level was added (IGELOO).FindingsResults highlight the importance of considering both resources as well as constraints in examining managers' mental health. Overarching context, organizational constraints and the management of difficult employees played important roles in the stress experienced by managers.Practical implicationsThe results offer a better understanding of the importance of intervening at different levels to promote better organizational health. Results also highlight the importance of setting up organizational resources and act on the various constraints to reduce them. Different individual strategies used by managers to deal with the various constraints and maintain their mental health also emerge from those results.Originality/valueIn addition to addressing the reality of healthcare managers, this study supplements a theoretical model and suggests avenues for interventions promoting more sustainable organizational health.
Why can’t we be friends? Bridging the academic/practitioner gap in social marketing
Authors: Foote Liz , Sherring Phill , Rundle-Thiele Sharyn 
Published: November 2023

Purpose
In this paper we (a pracademic, a practitioner, and an academic) aim to explore the academic/practitioner gap in social marketing and offer recommendations to close it, while amplifying existing examples of best practice from within the field. We also propose a research agenda to spur dialog and guide further investigations in this area. Insights from prior research, coupled with the co-authors’ experience and observations, indicate that a disconnect does exist between academia and practice within social marketing, though it is admittedly and unsurprisingly not uniform across contexts and disciplinary areas. Given social marketing’s identity as a practice-oriented field, there are many existing examples of academic/practitioner collaboration and the successful linkage of theory and practice that deserve to be amplified. However, the challenges associated with the very different systems and structures affecting both worlds mean the disconnect is problematic enough to warrant systematic change to ensure the two worlds are more aligned.

Design/methodology/approach
This paper (a pracademic, a practitioner and an academic) explores the academic/practitioner gap in social marketing and offer recommendations to close it, while amplifying existing examples of best practice from within the field. The authors also propose a research agenda to spur dialog and guide further investigations in this area.

Findings
The authors suggest five key reasons that focus should be placed upon closing the academic/practitioner gap in social marketing: demonstrating societal value by contributing to practice; embedding and developing theories in practice; adding to the social marketing literature; contributing to social marketing teaching; and communicating the value and effectiveness of social marketing. To close the gap, the authors propose specific recommendations within four broad areas: marketing the academia and practitioner collaboration offer; building ongoing relationships; creating collaborative partnerships; and changing the publishing model ensuring communications are accessible to all. They also suggest ways for social marketing associations and peak bodies to play a role.

Originality/value
The concept of a disconnect between academia and practice is by no means new; it has been a pervasive issue across disciplines for decades. However, this issue has not been the subject of much discussion within the social marketing literature. Recommendations outlined in this paper serve as a starting point for discussion. The authors also acknowledge that due to long standing “bright spots” in the field, numerous examples currently exist. They place an emphasis upon highlighting these examples while illuminating a path forward.
How do transport companies execute strategies in a volatile environment? A qualitative inquiry
Authors: Ssekiziyivu Bob , Bagire Vincent , Ngoma Muhammed , Nkurunziza Gideon , Abaho Ernest , Hassan Bashir 
Published: November 2023

PurposeThe purpose of this study was to explore how transport companies in Uganda execute strategies in a turbulent business environment.Design/methodology/approachThe study adopted an exploratory qualitative methodology using the data collected through an open-ended instrument. Utilizing the qualitative data analysis software QSR NVivo9, the data were analyzed following the Gioia's methodology. Verbatim texts were used to explain the emergent themes.FindingsThe study's findings show that to successfully execute strategies, companies in Uganda communicate, coordinate and put control systems in their operations. The activities undertaken include customer care, timely settlement of complaints, comfortable seats, playing local music, partnerships with reliable fuel stations, setting up strategic offices, cost management, use of experienced drivers, sub-renting vehicles and inspections.Originality/valueThe study produces a pioneering result of how transport companies execute strategies in a turbulent business environment, an aspect that has not been adequately highlighted in previous studies.
Bots for mental health: the boundaries of human and technology agencies for enabling mental well-being within organizations
Authors: Dutta Debolina , Mishra Sushanta Kumar 
Published: November 2023

PurposeThe importance of mental wellbeing and the need for organizations to address it is increasing in the post-pandemic context. Although Artificial Intelligence (AI) is increasingly being adopted in HRM functions, its adoption and utility for enabling mental wellbeing is limited. Building on the Open System Theory (OST) and adopting the technology-in-practice lens, the authors examined the roles of human and technology agencies in enabling mental wellbeing.Design/methodology/approachThe study was conducted in two stages; in Stage 1, the authors adopted a case methodology approach to examine the feasibility of a technology company's offerings to assess mental wellbeing. In Stage 2, the authors followed the grounded theory approach and interviewed 22 key stakeholders and HR leaders of diverse organizations. The authors used Gioia's approach to analyze the data.FindingsThe study demonstrates the interdependence and inseparability of human activity, technological capability and structured context. Specifically, the authors observe that AI adoption is pushing the boundaries of how organizations could support employees' mental health and wellbeing. These technological advancements and adoption are likely to facilitate the evolution of agentic practices, routines and structures.Research limitations/implicationsThis study carries two important implications. While the advent of cutting-edge technologies appears to affect employees' mental wellbeing, the study findings indicate the assistive role of technology in supporting mental wellbeing and facilitating changes in organizational practices. Second, the ontology of technology-in-practice shows how human–machine agencies gain newer relevance from the interactions that unite them. Specifically, per OST, technology (from an external context) can potentially change how mental wellbeing practices in organizations are managed. The authors extend the existing literature by suggesting that both human agents and internal contexts effectively limit the potential of technology agents to change existing structures significantly.Originality/valueThe authors address the need for more research on the technology-management interface, and the boundaries of technology-enabled wellbeing at work. While AI-HRM scholarship has primarily relied on micro-level psychological theories to examine impact and outcomes, the authors borrow from the macro-level theories, such as the OST and the technology-in-practice to explain how AI is shifting the boundaries of human and machine agencies for enabling mental wellbeing.