bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Liver isoform of phosphofructokinase (PFKL)-Mediated Hypoxic Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells Attenuate Cardiac Arrest-Induced Pyroptosis in Rat Cortical Neurons by Protecting Mitochondrial Function from Oxidative Damage
Authors: Tang Xiahong , Zheng Nan , Gong Zheng , You Yan , Lin Qingming , Zhuang Yangping , Wu Jiali , Wang Yu , Huang Hanlin , Chen Feng , Ke Jun 
Published: November 2023

Abstract
Introduction: Cardiac arrest (CA) often leads to severe neurological dysfunction due to inflammation, mitochondrial dysfunction, and post-cardiopulmonary resuscitation (CPR) neurological damage. Bone marrow-derived mesenchymal stem cells (BMSCs) show promise for neurological diseases, but optimizing their therapeutic potential and neuroregulation post-CA remains unclear.
Methods: We established an in vitro co-culture model with BMSCs and post-oxygen-glucose deprivation (OGD) primary neurons, confirming that hypoxic preconditioning enhances BMSCs' resistance to neuronal pyroptosis. We induced an 8-minute CA model through asphyxia induction and assessed hypoxic preconditioned bone marrow-derived mesenchymal stem cells (HP-BMSCs) on post-resuscitation neuronal mitochondrial oxidative stress and pyroptosis using neurological deficit scores (NDS), brain tissue oxidative stress markers, apoptosis-related proteins, mitochondrial area, and damage markers. Mechanistic studies knocked down PFKL expression in HP-BMSCs via si-RNA, verifying potential mechanisms in animals and cells.
Results: Hypoxic preconditioning boosted BMSCs' neuroprotective effect against neuronal pyroptosis, possibly through MAPK and NF-κB pathway inhibition. Consequently, we pursued HP-BMSCs as a neuroprotection strategy, with RNA sequencing suggesting liver isoform of phosphofructokinase (PFKL) as a regulatory molecule. HP-BMSCs significantly reduced neuronal pyroptosis, oxidative stress, and mitochondrial damage induced by CA. This manifested as improved oxidative stress markers, decreased apoptosis-related protein levels, enhanced cell membrane and mitochondrial structures, and reduced mitochondrial damage markers. Transfection of PFKL-targeted si-RNA into HP-BMSCs weakened their protective effects. We also established an in vitro co-culture model to confirm HP-BMSCs' role in improving neuronal energy metabolism following OGD. HP-BMSCs lowered apoptosis-related protein levels and mitochondrial damage markers in primary neurons. Intracellular and mitochondrial reactive oxygen species (ROS) levels dropped, as detected by DCFH-DA and MitoSOX probes. Notably, knocking down PFKL expression in HP-BMSCs reversed these protective effects.
Conclusion: In conclusion, HP-BMSCs offer a promising therapeutic approach for brain injury post-CA by reducing cell pyroptosis mediated by mitochondrial ROS, potentially linked to elevated PFKL expression following hypoxic preconditioning.
Notch signaling pathway promotes invasion of ameloblastoma by regulating E-cadherin via cyclinD1
Authors: Gabiyatu Baoyinbatu , Li Wenchao , Amuersana Wurihan , Tian Yuan , Li Senhao , Tsagaankhuu Sarnai , lidao Bao , Boldbaatar Damdindorj 
Published: November 2023

Abstract
This study aims to investigate the impact of the Notch signaling pathway on the invasion of Ameloblastoma (AM) and its influence on the expression of cyclinD1 protein. AM cells and dental follicle (DF) cells were cultured in vitro. The proliferation activity of two cells were measured using the CCK8. The invasive ability of cells were assessed using the Transwell chamber. A comparison was made between the proliferation and invasion abilities of two cells. The protein expression of cyclinD1, E-cadherin, and Snail was detected using Western Blot. The Notch signaling pathway was blocked using the Notch inhibitor FLI-06.The comparison of proliferation and invasion abilities between two cells were higher than control group cells.The difference was statistically significant (P＜0.05). The Notch inhibitor on the invasive ability of AM cells is significantly lower than control group. The difference was statistically significant (P＜0.05). After intervention with FLI-06, the protein expression of CDK 1, cyclinD1, and Snail in AM cells showed a significant downregulation, while the protein expression of E-cadherin showed a significant upregulation.The Notch signaling pathway plays a crucial role Notch signaling pathway promotes invasion ofAM by regulating E-cadherin via cyclinD1.
Crystal Growth and Characterization of Single Crystals of Glycine doped with Ammonium Bicarbonate (GABIC) Crystal
Authors: Jayachithra M. , Meena M. , Sundararajan R. S. , Shalini M. , Girisun T. C. Sabari , Manikandan E. 
Published: November 2023

Abstract

At room temperature, a suitable conventional technique is used in the formation of a semi-organic single crystal of glycine that had been combined with ammonium bicarbonate (GABIC). Spectroscopic analysis is used to study the generated single crystal of ammonium bicarbonate complexed glycine. Based on the results of single crystal X-ray diffraction examinations, the monoclinic system is determined. The FT-IR spectral research was the method that led to the identification of the many functional groups that are presented in GABIC. Both the UV-visible spectrum and the fluorescence spectrum provide information on the optical and electrical characteristics of the formed crystal, respectively. The optical band gap has been calculated to be 4.49 eV after taking into account the UV absorption curve. A number of optical parameters, such as the extinction coefficient and the reflectance, were also measured and calculated. Vickers microhardness experiments were used to determine the mechanical stability of the generated crystal, and the results showed that the crystal clearly belonged to the soft material group. In order to quantify the material's hardness number (Hv), Meyer's index (n), yield strength (σy), elastic stiffness constant (C11), brittle index (Bi), and fracture toughness (Kc) a Vickers microhardness test was performed on one of the as-grown hard crystals (Hv). Constant frequency measurements of the formed crystal's dielectric constant and dielectric loss were taken. By using a scanning electron microscope, we were able to figure out the morphology of the material's surface. The Nd: YAG laser has been used to assess the damage threshold energy required to cause permanent damage (1064 nm). With the use of the open aperture Z-scan method (Nd: YAG, 532 nm, 9 ns), we are able to determine that the nonlinearity coefficient (β) is 0.95 1010 m/W, which is the outcome of an effective two-photon absorption phenomena. With a fluence threshold of 3.26 1012 W/m2, the GABIC's consequent optical limiting characteristic makes it a promising option for use in optical limiting devices. The creation of GABIC was shown by these experiments, and Glycine was found to be a critical factor in the observed modifications to the crystals' spectral, optical, and mechanical characteristics.
Research on Network Ideology Risk Modeling and Governance Strategy in Big Data Environment
Authors: Yin Laiye , Hu Bangning , Yan Zeng 
Published: November 2023

Abstract
With the rapid development of computer network technology and the improvement of social information, computer network plays an increasingly important role in the fields of politics, economy, military and life. Network ideology security is facing new risks and challenges under the big data environment, which also provides new solutions for network ideology risk management. In the big data environment, network ideology security faces three risks: subject, information and environment. Using the hierarchical holographic modeling method, we deconstruct the subject, information and environment layer by layer, and build a three-level index framework of network ideology risk This paper combines network social governance with the era of big data, constructs the framework of network social governance under the influence of big data, and based on this framework, puts forward the optimization path of network social governance under the influence of big data, which broadens the research dimension of traditional network social governance path .The lr-nsrpm method proposed in this paper uses logic language to describe network system parameters, which reduces the CPU and ram utilization by 21.65% compared with the existing working methods .Construct the risk framework of network ideology, identify observable risk indicators, quantify and rate the indicators, and conclude that the value orientation, information content, information literacy and emotional stability of Internet users are high-risk indicators of network ideology.
Correction of temperature effects in dielectric response tests in power transformers using artificial intelligence
Authors: Mousavi Seyed Amidedin 
Published: November 2023

Abstract
Polarization and Depolarization Current (PDC) and Frequency Domain Spectroscopy (FDS) measurements are common dielectric response tests in time and frequency domain that are widely used to diagnosis insulation status in power transformer companies. Numerous factors affect the PDC and FDS test results, which the most important of them is temperature variation. To accurately interpret the results of the dielectric response, the effect of temperature on the results in time and frequency domains, must be corrected in order to make a proper assessment of the condition of the insulation system. In this paper, by using the correlation between the dielectric response results in time and frequency domain, the temperature effect in the PDC test results is corrected with the help of the temperature correction in FDS results.
Bioinformatics analysis and experimental verification of NLRX1 as a prognostic factor for esophageal squamous cell carcinoma
Authors: Zhou Lu , Gan Lanlan , Sun Chen , Chu Alan , Yang Menglin , Liu Zongwen 
Published: November 2023

Abstract
Background
NOD-like receptor X1 (NLRX1), a member of the nucleotide binding and oligomeric domain (NOD) like receptor (NLR) family, is connected to the physiological and pathological processes of inflammation, autophagy, immunity, metabolism, and mitochondrial regulation. It has been discovered to have pro- or anti-tumor effects in various tumor types. However, the biological function of NLRX1 in esophageal squamous cell carcinoma (ESCC) is still unknown.
Methods
Using bioinformatics methods, the differential expression of NLRX1 at the mRNA level was examined. OS analysis, clinical correlation analysis, ROC analysis, Cox analysis, coexpression analysis, enrichment analysis, immune infiltration analysis, and drug sensitivity analysis were carried out. A nomogram and calibration curve were constructed. Investigating changes in protein expression levels using IHC and WB, assessing the impact on proliferation using CCK-8 assays, examining migration using scratch healing assays, examining migration and invasion using transwell assays, and analyzing apoptosis using fluorescence and flow cytometry.
Results
Compared to normal tissue adjacent to cancer, NLRX1 is low expressed in ESCC, and patients with low NLRX1 expression have a shorter survival period. NLRX1 is an independent prognostic factor for ESCC and is connected to tumor grading. The low NLRX1 group showed a decrease in NK cells activated, Monocytes, and Macrophages M0 infiltration, and these immune cell infiltration levels were positively correlated with NLRX1 expression. Knocking down NLRX1 promotes the growth and development of KYSE450 cells, while overexpression of NLRX1 inhibits the growth and development of ECA109 cells. NLRX1 negatively regulates the PI3K/AKT pathway in ESCC.
Conclusions
Through several mechanisms, NLRX1 suppresses tumor growth in ESCC. This offers a fresh viewpoint for investigating the causes and progression of ESCC as well as for searching for more potent therapeutic approaches.
Impairment of cerebral vascular reactivity and resting blood flow in early-staged transgenic AD mice: in vivo optical imaging studies
Authors: Jeong Hyomin , Pan Yingtian , Akhter Firoz , Volkow Nora D. , Zhu Donghui , Du Congwu 
Published: November 2023

Abstract
Background
Alzheimer’s disease (AD) is a neurodegenerative disorder with progressive cognitive decline in aging individuals that poses a significant challenge to patients due to an incomplete understanding of its etiology and lack of effective interventions. While “the Amyloid Cascade Hypothesis,” the abnormal accumulation of amyloid-β in the brain, has been the most prevalent theory for AD, mounting evidence from clinical and epidemiological studies suggest that defects in cerebral vessels and hypoperfusion appear prior to other pathological manifestations and might contribute to AD, leading to “the Vascular Hypothesis.” However, assessment of structural and functional integrity of the cerebral vasculature in vivo in the brain from AD rodent models has been challenging owing to the limited spatiotemporal resolution of conventional imaging technologies.
Methods
We employed two in vivo imaging technologies, i.e., Dual-Wavelength Imaging (DWI) and Optical Coherence Tomography (OCT), to evaluate cerebrovascular reactivity (CVR; responsiveness of blood vessels to vasoconstriction as triggered by cocaine) in a relatively large field of view of the cortex in vivo, and 3D quantitative cerebrovascular blood flow (CBF) imaging in living transgenic AD mice at single vessel resolution.
Results
Our results showed significantly impaired CVR and reduced CBF in basal state in transgenic AD mice compared to non-transgenic littermates in an early stage of AD progression. Changes in total hemoglobin (Δ[HbT]) in response to vasoconstriction were significantly attenuated in AD mice, especially in arteries and tissue, and the recovery time of Δ[HbT] after vasoconstriction was shorter for AD than WT in all types of vessels and cortical tissue, thereby indicating hypoperfusion and reduced vascular flexibility. Additionally, our 3D OCT images revealed that CBF velocities in arteries were slower and that the microvascular network was severely disrupted in the brain of AD mice.
Conclusions
These results suggest significant vascular impairment in basal CBF and dynamic CVR in the neurovascular network in a rodent model of AD at an early stage of the disease. These cutting-edge in vivo optical imaging tools offer an innovative venue for detecting early neurovascular dysfunction in relation to AD pathology and pave the way for clinical translation of early diagnosis and elucidation of AD pathogenesis in the future.
Research on Chemical Intermediates Spectral, Density functional theory calculations, molecular docking studies, antimicrobial, antioxidant, antidiabetic activities of the novel (E)-2-(3-(5-(3-nitrophenyl)furan-2- yl)acryloyl)-3,4-dihydronaphthalen-1(2H)-one
Authors: Ahamed J. Irshad , Presley S. I. Davis , Babu Venkatadri , .s Jayachitra 
Published: November 2023

Abstract
The title molecule (E)-2-(3-(5-(3-nitrophenyl)furan-2-yl)acryloyl)-3,4-dihydronaphthalen-1(2H)-one (ENFADH) was completed by combining aromatic aldehydes with methyl ketones using Claisen-Schmidt condensation. The NMR, FTIR, and UV-visible investigations were used to characterize the novel chalcones. To determine the geometric, electronic, spectroscopic features, a theoretical model was also built using Gaussian 09 supporting basis set of B3LYP/6-311 + + G (d,p) as well as this basis set was used to performed the geometry optimization, harmonic vibration simulations, molecule electrostatic potential (MEP), frontier molecular orbitals (FMOs), and Mulliken's population analysis. A scaling factor of 0.9600 results in a attractive correspondence between the title compound vibrational spectrum and the experimental IR spectrum. In this innovative effort of this synthesized molecule gives more in-vitro research, such as anti-oxidant, antibacterial and antidiabetic tests were conducted. It revealed significant bactericidal activity with a maximum zone of inhibition of (19.0 ± 1.7mm) at 2.5 g/ml against Pseudomonas aeruginosa (ATCC 27853), presented maximum DPPH scavenging trait ranging from (30.8 ± 1.3% to 81.2 ± 1.5%) against Vitamin C. Likewise, it showed maximum H2O2 scavenging trait ranging from (37.9 ± 2.5% to 75.8 ± 6.4%) against ascorbic acid. In Anti-diabetic activities, it demonstrated α-amylase inhibition of 20.9 ± 2.4to 73.5 ± 2.3% and α-glucosidase inhibition by display activity of (23.3 ± 1.5 to 79.7 ± 1.2 U/L). In-silico molecular docking investigates using Auto Dock 4.0 showed the active pocket of the 1HD2 protein to estimate genotoxicology. By molecular docking, the title molcule exhibited a higher binding interaction energy of -6.5 (Kcal/mol) against the target 1HD2 protein. The obtained results suggest that the ADME characteristics of the examined drugs were predicted using their retention data (RM0). These early outcomes might be used to help choose new medication candidates and were shown to have no mutagenic effects when tested for toxicity.
Elemental composition of Pinus contorta Dougl. and Pinus sylvestris L. in experimental plantation in the Syktyvkar Forest district of the Komi Republic
Authors: Пристова Т.А. , Федорков А.Л. 
Published: November 2023

Определена концентрация 9 элементов (N, Mg, Ca, K, Na, Mn, Fe, Al, S) в надземной части двух видов сосны Pinus contorta Dougl. и P. sylvestris L. в лесных культурах сосны скрученной средней тайги Республики Коми. Выявлено, что оба вида, как представители рода Pinus , имеют много общего в элементном составе. Установлено, что аккумулятивный ряд элементов для исследуемых видов идентичен и надземные части деревьев относятся к азотно-калиево-кальциевому или азотно-кальциево-калиевому типу химизма. Высокое суммарное содержание элементов характерно для хвои, низкое - для древесины обоих видов, при этом из элементов минерального питания около 50% приходится на N, из зольных элементов 64-78% - на Ca и K. Показано, что сосна скрученная имеет ряд особенностей химического состава, по сравнению с сосной обыкновенной. Основное различие проявляется в более высоком содержании минеральных элементов в древесине (за исключением Mn и K), коре (за исключением Ca) и живых ветвях сосны скрученной. Видоспецифичность сосны скрученной проявляется в более высокой аккумуляции Al и Na практически во всех надземных частях дерева. Особенностью сосны скрученной является более низкое потребление азота и калия ассимилирующими органами и более высокий уровеньнакопления минеральных элементов в древесине и стволовой коре по сравнению с сосной обыкновенной.
The concentrations of 9 elements (N, Mg, Ca, K, Na, Mn, Fe, Al, S) were determined in the aboveground parts of two species ( Pinus contorta Dougl and P. sylvestris L.) in the mixed plantation in the middle taiga of the Komi Republic. It was revealed that both species, as representatives of the genus Pinus , have much in common in their elemental composition. It has been established that the accumulative series of elements for the studied species is identical and the above-ground parts of the trees belong to the nitrogen-potassium-calcium or nitrogen-calcium-potassium type of chemistry. A high total content of elements is typical for needles, a low one for wood of both species, while about 50% of mineral nutrition elements are N, and 64-78% of ash elements are Ca and K. It is shown that lodgepole pine has a number of chemical features in composition, compared with Scots pine. The main difference is manifested in the higher content of mineral elements in perennial needles (excluding N and S), wood (excluding Mn and K) and bark (excluding Ca) of lodgepole pine. The species specificity of lodgepole pine is manifested in a higher accumulation of Al and Na in almost all above-ground parts of the tree. A feature of lodgepole pine is a lower consumption of nitrogen and potassium by assimilating organs and a higher level of accumulation of mineral elements in wood and stem bark compared to Scots pine.
Modeling the impact of climate change on streamflow responses in the Kessem watershed, Middle Awash sub-basin, Ethiopia
Authors: Assfaw Mamush Tekle , Neka Bogale Gebremariam , Ayele Elias Gebeyehu 
Published: November 2023

Abstract

In this study, we examined how future climate change will affect streamflow responses in the Kessem watershed. Climate variables from SSP2-4.5 and SSP5-8.5 emission scenarios were extracted from GCMs for the 2040s (2031–2060) and 2070s (2061–2090). The bias-corrected precipitation and temperature were converted into streamflow using a calibrated SWAT model. The simulated output of the future streamflow for the periods 2040s and 2070s was compared with the base period (1992–2020) and presented as percentage changes. During calibration and validation, the SWAT model showed Nash–Sutcliffe efficiency (NSE) values of 0.79 and 0.77, as well as coefficient of determination (R2) values of 0.8 and 0.79, demonstrating its capability of simulating streamflow. The annual mean maximum and minimum temperatures are predicted to increase, with a pronounced increase in the minimum temperature for the mid-term and long-term futures under both emission scenarios. As we approach the end of the century, we see an increase in annual mean rainfall and streamflow under the SSP5-8.5 emission scenario. The increment in annual mean rainfall (streamflow) is expected to be 3% (12.5%) and 23% (48.8%) for the 2040s and 2070s, respectively, under the SSP5-8.5 emission scenario.
River basin change index for integrated river basin management of Langat River, Malaysia
Authors: Lim Foo-Hoat , Lee Wei-Koon , Khor Wei-Sze , Chan Lina , Sulaiman Mohd Sofiyan , Shaari Hasrolnizam , Jalil Muhamad Khairi 
Published: November 2023

AbstractA river basin change index (RBCI) is introduced as a management tool to evaluate integrated river basin management (IRBM) implementation. The framework comprises water governance (WG), river basin organisation (RBO) and technical indicators. WG indicators encompass effectiveness, efficiency, trust and engagement. RBO indicators examine the management capacity and resources in operation and expansion. The technical indicators measure the outcome of IRBM implementation corresponding to each policy in the IRBM plan. Data sources range from questionnaire surveys, official reports and recorded data from the relevant agencies. The indices are calculated by comparing the current value to a reference value, which may be historical or target performance, or average performance of other river basins. RBCI value in the range of 0.9–1.1 characterises regular year-to-year fluctuation. A lower index value &lt;0.9 indicates negative basin change, which may be due to negligence, retardation or disastrous regress in the basin IRBM in deteriorating order. A higher index value &gt;1.1 indicates positive basin change which may be attributed to notable improvement, outstanding attainment or breakthrough advances in the basin IRBM in successive order. The tool is useful to facilitate long-term continuous improvement process and is presently undergoing pilot-testing for the Langat River basin, Malaysia