bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency
Authors: Guillardín Laura , MacKay John J. 
Published: October 2023

Abstract
Background
Genetic and genomic studies are seeing an increase in sample sizes together with a wider range of species investigated in response to environmental change concerns. In turn, these changes may come with challenges including the time and difficulty to isolate nucleic acids (DNA or RNA), the sequencing cost and environmental impacts of the growing amount of plastic waste generated in the process. Pseudotsuga menziesii var. menziesii (Mirbel) Franco (PM), Tsuga heterophylla (Raf.) Sarg. (TH) and Thuja plicata Donn ex D.Don (TP) are conifer species found in diverse woodlands both as natives and naturalized exotics. Our study was carried out whilst investigating their genetics to understand their population structure and potential for adaptation.

Results
In the present study, we compared two different DNA isolation methods, i.e., spin-column DNeasy plant mini kit (QIAGEN), and temperature-driven enzymatic cocktail Plant DNA Extraction (MicroGEM). The quantity of recovered DNA and the quality of DNA were assessed along with the plastic footprint and time needed for three tree species. Both methods were optimised and proven to provide enough DNA for each studied species. The yield of DNA for each method depended on the species: QIAGEN showed higher yield in P. menziesii and T. heterophylla, while T. plicata recovered similar amount of DNA for both methods. The DNA quality was investigated using DNA barcoding techniques by confirming species identity and species discrimination. No difference was detected in the PCR amplification of the two barcoding loci, (rbcL and trnH-psbA), and the recovered sequences between DNA isolation methods. Measurement of the plastic use and the processing time per sample indicated that MicroGEM had a 52.64% lower plastic footprint and was 51.8% faster than QIAGEN.

Conclusions
QIAGEN gave higher yields in two of the species although both methods showed similar quality results across all species. However, MicroGEM was clearly advantageous to decrease the plastic footprint and improve the time efficiency. Overall, MicroGEM recovers sufficient and reliable DNA to perform common downstream analyses such as PCR and sequencing. Our findings illustrate the benefits of research and efforts towards developing more sustainable methods and techniques to reduce the environmental footprint of molecular analyses.
The predictive factors of moral courage among hospital nurses
Authors: Hakimi Hamideh , Mousazadeh Noushin , Sharif-Nia Hamid , Nazari Roghieh , Dehghani Maryam 
Published: October 2023

Abstract
Background
Having moral courage is a crucial characteristic for nurses to handle ethical quandaries, stay true to their professional obligations towards patients, and uphold ethical principles. This concept can be influenced by various factors including personal, professional, organizational, and leadership considerations. The purpose of this study was to explore the predictors of moral courage among nurses working in hospitals.

Methods
In 2018, an observational cross-sectional study was carried out on 267 nurses employed in six hospitals located in the northern region of Iran. The participants were selected through a simple random sampling technique. To collect data, a demographic information form was used along with two questionnaires. The first questionnaire was a standard survey on moral courage, while the second questionnaire was designed to assess the ethical climate. Linear regression was used to assess the predictors of moral courage.

Results
Nurses had an average moral courage score of 87.07 ± 15.52 and an average moral climate score of 96.12 ± 17.17. The study showed that 16% of the variation in moral courage scores among nurses was explained by ethical climate and monthly overtime hours.

Conclusion
This study underscores the significance of establishing an ethical work environment and minimizing overtime hours in order to enhance moral courage among nurses. These findings carry weight for both nursing practice and organizational policies focused on fostering ethical conduct within healthcare settings.
Does the registry speak your language? A case study of the Global Angelman Syndrome Registry
Authors: Tones Megan , Zeps Nikolajs , Wyborn Yvette , Smith Adam , Barrero Roberto A. , Heussler Helen , Cross Meagan , McGree James , Bellgard Matthew 
Published: October 2023

AbstractGlobal disease registries are critical to capturing common patient related information on rare illnesses, allowing patients and their families to provide information about their condition in a safe, accessible, and engaging manner that enables researchers to undertake critical research aimed at improving outcomes. Typically, English is the default language of choice for these global digital health platforms. Unfortunately, language barriers can significantly inhibit participation from non-English speaking participants. In addition, there is potential for compromises in data quality and completeness. In contrast, multinational commercial entities provide access to their websites in the local language of the country they are operating in, and often provide multiple options reflecting ethnic diversity. This paper presents a case study of how the Global Angelman Syndrome Registry (GASR) has used a novel approach to enable multiple language translations for its website. Using a “semi-automated language translation” approach, the GASR, which was originally launched in English in September 2016, is now available in several other languages. In 2020, the GASR adopted a novel approach using crowd-sourcing and machine translation tools leading to the availability of the GASR in Spanish, Traditional Chinese, Italian, and Hindi. As a result, enrolments increased by 124% percent for Spain, 67% percent for Latin America, 46% percent for Asia, 24% for Italy, and 43% for India. We describe our approach here, which we believe presents an opportunity for cost-effective and timely translations responsive to changes to the registry and helps build and maintain engagement with global disease communities.
Forms of sexual violence perpetrated in conflict and post-conflict settings against South Sudanese men resettled in two communities in Uganda: an exploratory qualitative study
Authors: Olaluwoye Tosin , Hoban Elizabeth , Williams Joanne 
Published: October 2023

Abstract
Background
Consideration for men as survivors of sexual violence in conflict and post-conflict settings has gained some prominence in the last decade. There remains a paucity of empirical data on forms of sexual violence from the survivors’ perspective, and no study has considered the context of the 2013 South Sudan conflict specifically.

Methods
This paper reports the findings of an exploratory qualitative study on the forms of sexual violence perpetrated against men in conflict and post-conflict settings, with the survivors as the main participants. A purposive sampling technique was used to recruit 26 South Sudanese male sexual violence survivors who have resettled in two refugee resettlement communities in Uganda since the onset of the 2013 South Sudan conflict. In-depth semi-structured interviews were used to collect the data from the male survivors. Six humanitarian aid workers who support sexual violence survivors also participated as key informants. Thematic data analysis was performed on the qualitative data.

Results
The 26 survivors reported experiencing eight direct and two indirect forms of sexual violence in conflict and post-conflict settings. The direct forms include (1) striping men naked; (2) male rape; (3) exchange of sex for favours; (4) forcing men to rape other people; (5) genital mutilation; (6) genital beating; (7) insertion of objects into men’s anus, and (8) taking men as wives. The indirect forms were forcing men to witness the rape of a female relative and forcing men to cheer or assist during the rape of other people.

Conclusion
To maximize positive health outcomes for survivors, stakeholders must consider both direct and indirect forms of male-directed conflict-related sexual violence in policy and practice.
Green synthesis of highly functionalized heterocyclic bearing pyrazole moiety for cancer-targeted chemo/radioisotope therapy
Authors: Anwer Kurls E. , Sayed Galal H. , Essa Basma M. , Selim Adli A. 
Published: October 2023

AbstractNew derivatives of heterocyclic bearing pyrazole moiety were synthesized (eight new compounds from 2 to 9) via green synthesis methods (microwave-assisted and grinding techniques). 4,6-Diamino-1,3-diphenyl-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile (2) shows high anti-cancer activity against both HepG2 and HCT-116 with IC50of 9.2 ± 2.8 and 7.7 ± 1.8 µM, respectively, which referenced to 5-Fu which is showing activity of 7.86 ± 0.5 and 5.35 ± 0.3 against both HepG2 and HCT-116, respectively. The cytotoxic activity against HCT-116 and HepG2 was slightly decreased and slightly increased, respectively, by a different pyrazole moiety (compound 5). Pharmacokinetics of compound 2 was carried out using the radioiodination technique in tumour-bearing Albino mice which shows good uptake at the tumour site. The biodistribution showed high accumulation in tumour tissues with a ratio of 13.7% ID/g organ after one hour in comparison with 2.97% ID/g organ at normal muscle at the same time point. As I-131 has maximum beta and gamma energies of 606.3 and 364.5 keV, respectively, therefore the newly synthesized compound 2 may be used for chemotherapy and TRT.
Janus kinase inhibitor, tofacitinib, in refractory juvenile dermatomyositis: a retrospective multi-central study in China
Authors: Zhang Junmei , Sun Li , Shi XinWei , Li Shipeng , Liu Cuihua , Li Xiaoqing , Lu Meiping , Deng Jianghong , Tan Xiaohua , Guan Wanzhen , Li Guomin , Wen Xinran , Liu Ping , Li Caifeng 
Published: October 2023

Abstract
Objectives
Juvenile dermatomyositis (JDM) is a chronic autoimmune disease. Some patients remain in an active state even though they were administrated with a combination of corticosteroid and methotrexate. Existing research has suggested that interferon and Janus kinase played an important role in pathogenesis. Existing research has suggested the efficacy of JAK inhibitors (JAKi). Our retrospective study aimed to investigate the efficacy of tofacitinib in refractory JDM patients.

Methods
A total of eighty-eight patients in China who had been diagnosed with JDM and subjected to tofacitinib therapy for over 3 months were retrospectively analyzed. Skin and muscle manifestations were assessed using the Cutaneous Assessment Tool-binary method (CAT-BM), Childhood Myositis Assessment Scale (CMAS), and kinase. Pulmonary function was assessed using a high-resolution CT (computerized tomography) scan and pulmonary symptoms. All patients were subjected to regular follow-up, and core measures were assessed every 3 months after initiation. Furthermore, the data were analyzed using the Wilcoxon single test, Mann–Whitney U test, and chi-square test.

Results
Compared with the baseline data, skin and muscle manifestations were found significantly improved during the respective follow-up visit. At the most recent follow-up, nearly 50% of patients achieved a clinical complete response and six patients received tofacitinib monotherapy. Sixty percent of patients suffering from interstitial lung disease well recovered on high-resolution CT. Seventy-five percent of patients showed a reduction in the size or number of calcinosis, and 25% of patients showed completely resolved calcinosis.

Conclusion
In this study, the result suggested that tofacitinib therapy exerted a certain effect on skin manifestations, muscle manifestations, interstitial lung disease (ILD), calcinosis, as well as downgrade of medication. In-depth research should be conducted to focus on the correlation between the pathogenesis of JDM and JAKi.
Radiation impacts gene redundancy and biofilm regulation of cryoconite microbiomes in Northern Hemisphere glaciers
Authors: Zhang Zhihao , Liu Yongqin , Zhao Weishu , Ji Mukan 
Published: October 2023

AbstractBackgroundGlaciers harbor diverse microorganisms adapted to extreme conditions with high radiation, fluctuating temperature, and low nutrient availability. In glacial ecosystems, cryoconite granules are hotspots of microbial metabolic activity and could influences the biogeochemical cycle on glacier surface. Climate change could influence glacier dynamics by changing regional meteorological factors (e.g., radiation, precipitation, temperature, wind, and evaporation). Moreover, meteorological factors not only influence glacier dynamics but also directly or indirectly influence cryoconite microbiomes. However, the relationship of the meteorological factors and cryoconite microbiome are poorly understood.ResultsHere, we collected 88 metagenomes from 26 glaciers distributed in the Northern Hemisphere with corresponding public meteorological data to reveal the relationship between meteorological factors and variation of cryoconite microbiome. Our results showed significant differences in taxonomic and genomic characteristics between cryoconite generalists and specialists. Additionally, we found that the biogeography of both generalists and specialists was influenced by solar radiation. Specialists with smaller genome size and lower gene redundancy were more abundant under high radiation stress, implying that streamlined genomes are more adapted to high radiation conditions. Network analysis revealed that biofilm regulation is a ubiquitous function in response to radiation stress, and hub genes were associated with the formation and dispersion of biofilms.ConclusionThese findings enhance our understanding of glacier cryoconite microbiome variation on a hemispheric scale and indicate the response mechanisms to radiation stress, which will support forecasts of the ecological consequences of future climate change.
The relationship between cannabis and anorexia nervosa: a scoping review
Authors: Rogers Chloe I. , Pacanowski Carly R. 
Published: October 2023

Abstract
Background
Relapse rates in Anorexia Nervosa (AN) remain high, warranting exploration of further treatments. Cannabinoid agonists are of interest as they have shown successful outcomes in the treatment of associated conditions, such as post-traumatic stress disorder. This scoping review explores the endocannabinoid system (ECS), benefits/harms/null effects of cannabinoid treatment, and harms of cannabis use in AN.

Methods
PubMed, PsycINFO, Cochrane, and Web of Science were searched for studies published between 2010 and August 2023, with human participants that explored the ECS, cannabinoid treatment, or cannabis use, and included 1 or more keywords for both cannabis and AN in the title and or abstract. Reports describing secondary anorexia, reports not available in English, grey literature, reports combining data from AN with other conditions, and reports only reporting the prevalence of cannabis abuse/dependence were excluded. Data were extracted from 17 reports (n = 15 studies). For the ECS, outcomes included genetics such as allele expression related to the ECS, cannabinoid receptor availability, and circulating levels of endocannabinoids. For benefits/harms/null effects of cannabinoid treatment, outcomes included changes in weight, eating disorder (ED) symptoms, physical activity (PA), and hormones. For harms of cannabis use, outcomes included genetics related to cannabis use disorder and associations between cannabis use and ED symptoms.

Results
Eight studies (n = 8 reports) found abnormalities in the ECS in AN including expression of related alleles, genotypes, and haplotypes, availability of cannabinoid receptors, and levels of endocannabinoids. Three studies (n = 5 reports) found benefits/harms/null effects of cannabinoid treatment. Benefits included weight gain, improved ED symptoms and reduced PA, while null effects included no changes in weight or ED symptoms, and harms included increased PA and lowered adipose hormones. Four studies (n = 4 reports) expanded upon harms of cannabis use, including genetic predispositions to cannabis use disorder, and compensatory behaviors related to cannabis use.

Conclusion
Limited evidence suggests that abnormalities in the ECS in AN may render cannabis a potential treatment for weight restoration and associated symptoms. Future research may wish to investigate individualized dosing approaches to maximize beneficial effects while minimizing harms.
Level II Evidence: Scoping Review.
Relationship between career maturity, psychological separation, and occupational self-efficacy of postgraduates: moderating effect of registered residence type
Authors: Ni Jianchao , Zhang Jiawen , Wang Yumei , Li Dongchen , Chen Chunmei 
Published: October 2023

Abstract
Background
With the slowdown of economic growth and the increasing pressure of employment competition worldwide during the normalized epidemic prevention and control, the job-hunting intention and behavior of college graduates deserve in-depth study. This study explores the relationship between the career maturity, psychological separation and occupational self-efficacy of postgraduates, and provides a theoretical basis for improving their career maturity.

Methods
A questionnaire survey was carried out on postgraduates with 584 valid data in China by using the Career Maturity Scale, Psychological Separation Scale and the Occupational Self-efficacy Scale. A structural equation model and bias-corrected self-sampling method were adopted to explore their relationship. The moderating effect of registered residence type was tested.

Results
The results show that: (1) The higher the level of psychological separation of postgraduates, the higher their career maturity. (2) Occupational self-efficacy plays a mediating role in the process of psychological separation promoting career maturity. (3) The registered residence type moderates the latter half of the mediating process of psychological separation, occupational self-efficacy, and career maturity. Moreover, occupational self-efficacy plays a more significant role in promoting the career maturity of postgraduates with rural registered residence.

Conclusions
This study reveals the relationship between the career maturity, psychological separation and occupational self-efficacy of postgraduates. At the same time, it also verifies the mediating role of occupational self-efficacy and the moderating role of registered residence type. The result is helpful for postgraduates to understand the level of their career maturity and improve their career decision-making level and career development ability.
Validation of the Chinese version of the Rosenberg Self-Esteem Scale: evidence from a three-wave longitudinal study
Authors: Jiang Chen , Zhu Yihong , Luo Yi , Tan Chee-Seng , Mastrotheodoros Stefanos , Costa Patrício , Chen Li , Guo Lina , Ma Haiyan , Meng Runtang 
Published: October 2023

Abstract
Background
The 10-item Rosenberg Self-Esteem Scale (RSES) is a widely used tool for individuals to self-report their self-esteem; however, the factorial structures of translated versions of the RSES vary across different languages. This study aimed to validate the Chinese version of the RSES in the Chinese mainland using a longitudinal design.

Methods
A group of healthcare university students completed the RSES across three waves: baseline, 1-week follow-up, and 15-week follow-up. A total of 481 valid responses were collected through the three-wave data collection process. Exploratory factor analysis (EFA) was performed on the baseline data to explore the potential factorial structure, while confirmatory factor analysis (CFA) was performed on the follow-up data to determine the best-fit model. Additionally, the cross-sectional and longitudinal measurement invariances were tested to assess the measurement properties of the RSES for different groups, such as gender and age, as well as across different time points. Convergent validity was assessed against the Self-Rated Health Questionnaire (SRHQ) using Spearman’s correlation. Internal consistency was examined using Cronbach’s alpha and McDonald’s omega coefficients, while test–retest reliability was assessed using intraclass correlation coefficient.

Results
The results of EFA revealed that Items 5, 8, and 9 had inadequate or cross-factor loadings, leading to their removal from further analysis. Analysis of the remaining seven items using EFA suggested a two-factor solution. A comparison of several potential models for the 10-item and 7-item RSES using CFA showed a preference for the 7-item form (RSES-7) with two factors. Furthermore, the RSES-7 exhibited strict invariance across different groups and time points, indicating its stability and consistency. The RSES-7 also demonstrated adequate convergent validity, internal consistency, and test–retest reliability, which further supported its robustness as a measure of self-esteem.

Conclusions
The findings suggest that the RSES-7 is a psychometrically sound and brief self-report scale for measuring self-esteem in the Chinese context. More studies are warranted to further verify its usability.
Evaluation of stability and functionality of zinc oxide nanofluids for enhanced oil recovery
Authors: Tuok Lengu Peter , Elkady Marwa , Zkria Abdelrahman , Yoshitake Tsuyoshi , Nour Eldemerdash Usama 
Published: October 2023

AbstractNanofluids for enhanced oil recovery offer a breakthrough solution towards tertiary recovery and consequently higher oil production. Their ability to reduce interfacial tension, alteration of formation’s wettability, higher adsorption capacity, and acceleration of disjoining pressure makes them excellent candidates for enhanced oil recovery. The main objective of this paper is to investigate the effect of polymers on zinc oxide (ZnO) nanofluids for enhanced oil recovery (EOR) and the role played by chemical modification using polymer stabilizers on nanoparticle stability in nanofluids. Nanoparticles with an average particle size of 34 nm were synthesized and used to prepare nanofluids of different concentrations and their stability was evaluated using sedimentation and UV–vis spectrophotometry tests. ZnO-synthesized nanofluids were used solely and in addition to Polyvinylpyrrolidone (PVP) and Polyvinyl alcohol (PVA) as stabilizing agents. It was noted that ZnO nanofluids with PVA stabilizer recorded the highest oil recovery of 82%. In contrast, the ZnO nanofluids without stabilizing agents registered the lowest recovery rate during the flooding experiment. The results revealed that a higher injection rate increases the oil recovery and reduces the viscous fingering effect with a better displacement front. Furthermore, nanofluids containing polymeric stabilizing agents achieved better recovery factors compared to ZnO nanofluids without stabilizing agents. This phenomenon was also observed in the interfacial tension test where nanofluids with PVA and PVP stabilizers reduced the IFT by 59% and 61% respectively.