bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal microbial composition associated with testosterone in the development of Meishan male pigs
Authors: Jiang Xueyuan , Deng Shaoshan , Lu Naisheng , Yao Wen , Xia Dong , Tu Weilong , Lei Hulong , Jia Peng , Gan Yeqing 
Published: November 2023

IntroductionThe gut microbiota closely relates to host health, whereas the relationship between gut microbiota and testosterone during the development of Meishan male pigs remains unclear. This study investigated the fecal microbiota composition and testosterone level during development in Meishan male pigs.MethodsFresh fecal samples of 20 healthy Meishan male pigs were individually collected at 10 and 22 weeks (wk) of age for testosterone content detection and bacteria pyrosequencing analysis. Anaerobic culture experiment of fecal bacteria in vitro was performed for bacteria pyrosequencing analysis.ResultsThe fecal testosterone content increased significantly from 10 weeks (wk) to 22 wk of age (P &lt; 0.05). Meanwhile, the boars at 22 wk had a lower abundance of phylum Bacteroidetes and Proteobacteria, and genus Alloprevotella, Prevotella_1, Prevotellaceae_NK3B31_group, and Streptococcus in the fecal microbiota composition (P &lt; 0.05). but higher proportions of the phylum Actinobacteria, Firmicutes, Kiritimatiellaeota, and Tenericutes, and genus Clostridium_sensu_stricto_1, Muribaculaceae and Terrisporobacter than that at 10 wk (P &lt; 0.05), and the Firmicutes to Bacteroidetes ratio was higher at 22 wk than 10 wk (P &lt; 0.05). Moreover, the fecal testosterone level significantly correlated with the relative abundance of the phylum Actinobacteria, Firmicutes, and Tencuteseri, and genus Alloprevotella, Clostridium_sensu_stricto_1, Muribaculaceae, Prevotella_1 and Streptococcus. Furthermore, the in vitro experiments indicated that the abundance of the phylum Proteobacteria and genus Escherichia-Shigella reduced with the increase of supplemental testosterone level. In contrast, the proportion of Firmicutes phylum increased with additional testosterone levels.DiscussionTestosterone could modulate the microflora structure. Meanwhile, the bacteria could degrade the testosterone in a dose testosterone-dependent manner. These results provide us with new insights into the relationship between the gut microbiome and testosterone and the contributions of the gut microbiome in physiological regulation in response to gonad development.
Movement predictability modulates sensorimotor processing
Authors: Altermatt Miriam , Thomas Felix Alexander , Wenderoth Nicole 
Published: November 2023

IntroductionAn important factor for optimal sensorimotor control is how well we are able to predict sensory feedback from internal and external sources during movement. If predictability decreases due to external disturbances, the brain is able to adjust muscle activation and the filtering of incoming sensory inputs. However, little is known about sensorimotor adjustments when predictability is increased by availability of additional internal feedback. In the present study we investigated how modifications of internal and external sensory feedback influence the control of muscle activation and gating of sensory input.MethodsCo-activation of forearm muscles, somatosensory evoked potentials (SEP) and short afferent inhibition (SAI) were assessed during three object manipulation tasks designed to differ in the predictability of sensory feedback. These included manipulation of a shared object with both hands (predictable coupling), manipulation of two independent objects without (uncoupled) and with external interference on one of the objects (unpredictable coupling).ResultsWe found a task-specific reduction in co-activation during the predictable coupling compared to the other tasks. Less sensory gating, reflected in larger subcortical SEP amplitudes, was observed in the unpredictable coupling task. SAI behavior was closely linked to the subcortical SEP component indicating an important function of subcortical sites in predictability related SEP gating and their direct influence on M1 inhibition.DiscussionTogether, these findings suggest that the unpredictable coupling task cannot only rely on predictive forward control and is compensated by enhancing co-activation and increasing the saliency for external stimuli by reducing sensory gating at subcortical level. This behavior might serve as a preparatory step to compensate for external disturbances and to enhance processing and integration of all incoming external stimuli to update the current sensorimotor state. In contrast, predictive forward control is accurate in the predictable coupling task due to the integrated sensory feedback from both hands where sensorimotor resources are economized by reducing muscular co-activation and increasing sensory gating.
Systematic review: clinical characteristics of anti-N-methyl-D-aspartate receptor encephalitis
Authors: Zhao Xi , Teng Yuou , Ni Jingnian , Li Ting , Shi Jing , Wei Mingqing 
Published: November 2023

BackgroundThe number of reported cases of anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis has gradually increased since its discovery in 2007, while there are no uniform treatment guidelines.ObjectiveTo summarize the clinical characteristics of patients with anti-NMDAR encephalitis and to analyze the factors affecting the disease prognosis.MethodsA systematic analysis of medical records was conducted, and PubMed, Embase, and Cochrane Library were searched from January 1, 2011, to December 31, 2021. Data were extracted, analyzed, and recorded in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines.ResultsThis study included 472 case reports. Most patients had prodromal symptoms of about 2 weeks, including psychiatric symptoms (53.2%), flu-like symptoms (51.5%), and seizures (23.9%), among others. Poor prognoses were associated with patients who had autonomic instability (p = 0.010), central hypoventilation (p = 0.014), and ICU support (p = 0.002). Patients with a higher age of onset were more likely to develop central hypoventilation (OR 1.024, CI 1.006–1.042, p = 0.009), cognitive impairment (OR 1.023, CI 1.009–1.037, p = 0.001), and memory impairment (OR 1.034, CI 1.017–1.050, p &lt; 0.001), whereas patients with a lower age were more likely to have seizures (OR 0.979, CI 0.965–0.993, p = 0.003). In this study, 97.0% of patients received immunotherapy, with the most commonly used treatment regimen being intravenous methylprednisolone (IVGC) and intravenous immunoglobulin (IVIG). When compared with other treatment regimens, the IVGC+IVIG regimen (p &lt; 0.001) resulted in better prognoses.ConclusionWhen encountering patients with fever, headache, and initial psychiatric symptoms of unknown etiology, clinicians should test their CSF for antibodies to distinguish autoimmune encephalitis. Patients with autonomic instability, central hypoventilation, and ICU support had poorer prognoses. Clinicians should be aware that older patients are more likely to develop central hypoventilation, cognitive impairment, and memory impairment, while younger patients are more likely to develop seizures. The IVGC+IVIG treatment regimen has better prognoses than others. This study includes case reports, which have obvious selection bias, and there are no unified standards to measure the severity of the disease. Therefore, in the future, larger samples and randomized controlled trials are needed to evaluate the efficacy of different treatment regimens.
A novel splicing mutation identified in a DMD patient: a case report
Authors: Wen Yuting , Yang Luo , Shen Gan , Dai Siyu , Wang Jing , Wang Xiang 
Published: November 2023

BackgroundDuchenne muscular dystrophy (DMD, ORPHA:98896) is a lethal X-linked recessive disease that manifests as progressive muscular weakness and wasting. Mutations in the dystrophy gene (DMD) are the main cause of Duchenne muscular dystrophy.Case presentationThis study aims to determine novel mutations of DMD and help preimplantation genetic diagnosis (PGD) for family planning. Here present a 4-year-old Chinses boy with DMD, whole-exome sequencing (WES) was performed to identify the molecular basis of the disease. It was confirmed that the boy carried a novel hemizygous mutation of NC_000023.11(NM_004006.3): c.5912_5922 + 19delinsATGTATG in DMD which inherited from his mother. This led to the aberrant splicing of DMD which demonstrated by a minigene splicing assay and further resulted in the impairment of the dystrophy protein.ConclusionsOur study discovered a novel splicing mutation of DMD in a DMD patient, which expands the variant spectrum of this gene and provide precise genetic diagnosis of DMD for timely therapy. Meanwhile, this finding will supply valuable information for preimplantation genetic diagnosis.
Determinants of photochemical characteristics of the photosynthetic electron transport chain of maize
Authors: Liu Xiuping , Qiao Yunzhou , Zhou Wangming , Dong Wenxu , Gu Lianhong 
Published: November 2023

IntroductionThe photosynthetic electron transport chain (ETC) is the bridge that links energy harvesting during the photophysical reactions at one end and energy consumption during the biochemical reactions at the other. Its functioning is thus fundamental for the proper balance between energy supply and demand in photosynthesis. Currently, there is a lack of understanding regarding how the structural properties of the ETC are affected by nutrient availability and plant developmental stages, which is a major roadblock to comprehensive modeling of photosynthesis.MethodsRedox parameters reflect the structural controls of ETC on the photochemical reactions and electron transport. We conducted joint measurements of chlorophyll fluorescence (ChlF) and gas exchange under systematically varying environmental conditions and growth stages of maize and sampled foliar nutrient contents. We utilized the recently developed steady-state photochemical model to infer redox parameters of electron transport from these measurements.Results and discussionWe found that the inferred values of these photochemical redox parameters varied with leaf macronutrient content. These variations may be caused either directly by these nutrients being components of protein complexes on the ETC or indirectly by their impacts on the structural integrity of the thylakoid and feedback from the biochemical reactions. Also, the redox parameters varied with plant morphology and developmental stage, reflecting seasonal changes in the structural properties of the ETC. Our findings will facilitate the parameterization and simulation of complete models of photosynthesis.
Kuban School of Thought in Thoracic Surgery: History of Its Formation and Development
Authors: Redko A. N. , Polyakov I. S. , Romantsov V. V. , Bedoeva K. O. , Shevchuk D. D. , Porhanov V. A. 
Published: November 2023

Introduction: The article discusses formation and development of the Kuban school of thought in thoracic surgery. We reviewed the main aspects of the accumulation of knowledge and experience in thoracic surgery and formation of a scientific association of thoracic surgeons that further developed into a school of thought first regionally, then internationally.Objective: To analyze the main stages of the development of the Kuban school of thought in thoracic surgery through the prism of scholars who made major contributions to the formation of the school’s research concept, as well as through succession (transfer of scientific and medical experience).Materials and methods: Our methodology involved a comparative historical analysis of the most important stages of the development of the school of thought in thoracic surgery, a retrospective evaluation of the contributions made by the surgeons who transformed the surgical community, and a comprehensive analysis of research materials on this subject. We used retrospective, biographical, analytical, and comparative research methods.Results: We identified the fundamental trends in the development of the Kuban school of thought in thoracic surgery, key directions for improving the methods of chest pathology diagnosis and surgical treatment that have been forming in the Kuban over the past 100 years.The education and training of staff, as well as formation of a scientific medical community and its international integration clearly demonstrated the transformation dynamics of the Krasnodar Krai’s research potential in thoracic surgery.
PENGARUH LABA BERSIH DAN ARUS KAS TERHADAP HARGA SAHAM PADA PERUSAHAAN SUB SEKTOR KIMIA YANG TERDAFTAR DI BURSA EFEK INDONESIA
Authors: Aisyah Rina Nur , Damayanti Reina , Lilianti Emma 
Published: November 2023

This study aims to determine the effect of Net Profit, Operating Cash Flow (AKO), Investing Cash Flow (AKI), and Financing Cash Flow (AKP) on Stock Prices of the chemical sector listed on the BEI for the 2016-2020 period with a population of 12 companies and 45 samples for 5 years. The data analysis in this study is a statistical analysis by collecting data through the data testing stage, namely: Normality Test, Multicollinearity Test, Heteroscedasticity, Auto-correlation Test, Regression Analysis Method, and Hypothesis Testing Test (partial and simultaneous) using the SPSS 25 software. The results of this study show that Net Income and Investing Cash Flow (AKI) have a significant effect on Stock Prices, meanwhile, Operating Cash Flow (AKO), and Financing Cash Flow (AKP) have no significant effect on Stock Prices. Net Profit, Operating Cash Flow (AKO), Investing Cash Flow (AKI) and Financing Cash Flow (AKP) simultaneously have significant effects on Stock Prices. Investing Cash Flow (AKI) is the dominant factor influencing Stock Price.
Keywords: Net Profit; Operating Cash Flow; Investing Cash Flow; Financing Cash Flow, Stock Prices
PENINGKATAN KINERJA DENGAN MELAKUKAN ANALISIS PERANCANGAN MENGGUNAKAN PENDEKATAN BALANCED SCORECARD PADA RSUD DI WILAYAH INDONESIA TIMUR (STUDI KASUS PADA RSUD DR.T.C.HILLERS MAUMERE)
Authors: Jelalu Oktaviana Florida 
Published: November 2023

The purpose of this research is to design the performance measurement by using the Balanced Scorecard approach at Dr.T.C.Hillers Maumere Hospital. A balanced scorecard helps the hospital achieve strategic targets from four perspectives: internally and externally. The methods that are used in collecting the data are observation, interview, and documentation. Based on the discussion result on four indicators of the Balanced Scorecard at Dr.T.C.Hillers Maumere Hospital shows the result of customer perspective which needs to be measured to increase patient satisfaction and loyalty, the financial perspective needs indicator to raise service revenue and cost control (streamline costs) so it can reach independent finance, internal business process perspective needs measurement to increase service innovation, measurement to increase basis service quality and reference, measurement to increase efficiency and effectiveness of service provision procedure and measurement to increase assets management, and then on growth and learning perspective by doing measurement to increase network technology utilization and quality human resources. Thus, it can be concluded that using the Balanced Scorecard as a performance measuring tool could help the hospital attain long-term success in a competitive market situation.
Keywords: Performance Measurement; Strategic Target; Balanced Scorecard; Regional Public Hospital
Comparison of quantitative and qualitative characteristics of the system “P-selectin — platelets — von Willebrand factor — metalloprotease ADAMTS13” in patients with sepsis, idiopathic thrombocytopenic purpura and healthy individuals
Authors: Koloskov A. V. , Divakova Yu. V. , Belyaeva E. L. , Tokareva I. P. , Karalevich S. A. , Chernova E. V. , Philippova O. I. , Avdeeva T. I. 
Published: November 2023

Introduction. The blood coagulation system plays a significant role in the pathogenesis of sepsis. Aim: to evaluate changes in the quantitative and qualitative characteristics of von Willebrand factor (vWF), ADAMTS13 metalloprotease and P-selectin in patients with sepsis, comparing them with those in patients with idiopathic thrombocytopenic purpura and in healthy individuals. Materials and methods. The study included 56 patients who received inpatient treatment due to the development of sepsis of various etiologies, 12 patients with idiopathic thrombocytopenic purpura (ITP) and 50 healthy volunteers. In a venous blood sample, the following studies were performed: vWF antigen (vWF:Ag), GPIb-binding ability of vWF (vWF:GPIb), binding ability of vWF to type I collagen (vWF:CBAI), binding ability of vWF to type III collagen I (vWF:CBAIII), P-selectin, ADAMTS13 antigen (ADAMTS13:Ag), ADAMTS13 activity (ADAMTS13:AC) and antibodies to ADAMTS13 (ADAMTS13:AB). Results. The median values of vWF:Ag, vWF:GPIb, vWF:CBAI, and vWF:CBAIII were significantly higher in patients with sepsis compared to healthy individuals and patients with ITP. The median P-selectin in patients with sepsis with thrombocytopenia 1213 ng/mL [1104–1302] was statistically significantly higher than in healthy individuals 74.5 ng/mL [65.0–84.5] (p = 0.0001), and with patients with ITP 389.0 ng/ml [276.2–589.2] (p = 0.0001). Median ADAMTS13:Ag in patients with sepsis with thrombocytopenia < 100×109/l 0.49 U/ml [0.44–0.71] and in patients with ITP 0.67 U/ml [0.62–0.76] were significantly lower compared with healthy individuals 0.97 U/ml [0.84–1.08] (p = 0.001 and p = 0.034, respectively), but the median ADAMTS13:Ag in patients with sepsis with thrombocytopenia was statistically significantly lower than in patients with ITP (p = 0.038). In 19.6 % of cases in patients with sepsis, the ADAMTS13:AB index was higher than 10.0 U/ml (19.6 U/ml [12.1–26.7]), while the median P-selectin indicator in this group of patients was significantly higher when compared with a group of patients with sepsis with an ADAMTS13:AB score of less than 10.00 U/mL (3.04 [2.11–3.94]): 1342 ng/mL[1271–1374] vs. 1130 ng/mL [1087–1271], p = 0.003, respectively. Conclusion. Activation of endothelial cells may underlie the increase in quantitative and qualitative indicators of vWF. Meanwhile, the effect of the adhesive characteristics of vWF (vWF:GPIb, vWF:CBAI and vWF:CBAIII) on the number of platelets in the blood of patients with sepsis was not revealed. Adhesion of vWF to the endothelium through interaction with P-selectin through the platelet GPIb receptor can promote the transition of vWF into a conformational-active state and lead to a decrease in the concentration of ADAMTS13 in the circulation.
Safety of plasma-derived blood products transfusions harvested from anti-SARS-CoV-2 positive and negative donors
Authors: Tikhomirov D. S. , Starkova O. G. , Ovchinnikova E. N. , Shaidurova K. V. , Demidov V. P. , Shishkanov D. V. , Tupoleva T. A. , Gaponova T. V. 
Published: November 2023

Introduction. Testing for antibodies to the causative agent COVID-19 is not mandatory for donor blood or its components. It is unknown whether transfusion of plasma-containing blood components from seropositive donors is a safe procedure especially for immunocompromised hematological patients. Aim. To assess the frequency of adverse reactions and complications in hematological patients after transfusions of plasma-derived blood products harvested from anti-SARS-CoV-2 positive and negative donors. Material and Methods. 9152 transfusions of components harvested from anti-SARS-CoV-2 positive and negative donors were carried out from April 29, 2020 to January 21, 2022 at the NMRC for Hematology. Post-transfusion reactions and complications were analyzed in 576 hematological patients. 2059 plasma units, 327 cryosupernatant plasma units and 6766 cryoprecipitate doses were assessed. The occurrence of adverse reactions (hyperthermia) and complications within two hours after transfusion were evaluated. Results. Among 9152 blood units, anti-SARS-CoV-2 were detected in 2660 (29 %) cases and 6492 (71 %) were antibodies free. The analysis of adverse reactions and complications showed no significant differences in the incidence of adverse reactions in recipients after anti-SARS-CoV-2 positive and negative blood unit transfusions. Conclusion. There were no significant differences in the number of post-transfusion reactions when using donor blood components (fresh frozen plasma, cryosupernatant plasma, cryoprecipitate) obtained from donors in whose blood antibodies to SARS-CoV-2 were detected or not detected. Plasma-derived blood products from seropositive donors with a high degree of probability can be used to correct the plasma hemostasis path in hematological patients along with components prepared from seronegative donors.
Successful treatment of relapses of primary mediastinal large B-cell lymphoma with central nervous system involvement
Authors: Koroleva D. A. , Gabeeva N. G. , Galstyan G. M. , Kovrigina A. M. , Yatsyk G. A. , Fedorova S. Yu. , Mangasarova Ya. K. , Zvonkov E. E. 
Published: November 2023

Introduction. Relapses of primary mediastinal large B-cell lymphoma (PMBСL) with involvement of the central nervous system (CNS) are usually incurable. Therapy tactics in patients with PMBCL with relapse in the CNS have not been defined. Aim: to present clinical observations of the treatment of relapses of PMBCL with CNS involvement using the “R-PML-CNS-2022” protocol. Main findings. Two clinical observations of patients with relapses of PMBCL with involvement of the CNS are presented. In both cases, the “R-PML—CNS-2022” protocol was used as a second-line treatment, and a complete remission of the disease was achieved. In the first case, within 6 months after auto-HCT, maintenance therapy with pembrolizumab was performed. Complete remission of the disease continues for 15 months. In the second observation, maintenance therapy with pembrolizumab was also started, however, due to the development of autoimmune complications after 2 cycles, treatment was discontinued. Complete remission of the disease continues for 13 months. Conclusion. The development of a new strategy for the treatment of relapses of PMLCL with CNS lesion according to the “R-PML—CNS-2022” protocol is a potentially curative approach in this group of patients.
Representasi Citra Diri Keanu Sebagai Influencer Melalui Instagram @keanuagl
Authors: Muhammad Rayhan Firdaus  , Rahmawati Zulfiningrum  
Published: November 2023

Salah satu platform media sosial yang populer adalah Instagram terlebih pada kalangan dewasa muda. Hingga muncul istilah “selebriti Instagram” dari penggunaan media sosial Instagram. Keanu Angelo atau @keanuagl merupakan selebgram atau influencer yang memiliki daya tarik tersendiri bagi kalangan remaja. Keanu Angelo juga merupakan influencer yang mempunyai karakter yang unik dengan cara berbicara yang lucu dan berpenampilan apa adanya atau sesuai dengan jati dirinya. Instagram @keanuagl masuk dalam “10 Selebgram Indonesia Paling Bersinar di 2020.” Tujuan dari penelitian ini adalah untuk menganalisis representasi citra diri Keanu sebagai influencer melalui Instagram @keanuagl. Metode penelitian menggunakan kualitatif dan analisis semiotika model Charles Sanders Pierce dengan teori segitiga makna yang terdiri dari objek, tanda dan makna. Hasil dari penelitian menunjukkan bahwa karakteristik representasi citra diri Keanu, mulai dari cara berpenampilan secara umum dalam Instagram, kebebasan berbusana sesuai karakter diri, percaya diri akan ukuran tubuh, penggunaan kosmetik untuk penunjang karir, dan model rambut yang mengikuti trend seluruh karakter tersebut direpresentasikan dalam Instagram @keanuagl. Citra diri Keanu menginspirasi influencer lain dan para pengikutnya di Instagram untuk menjadi diri sendiri dan memiliki karakter.