bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-Term Dendritic Dynamics of Neonatal Cortical Neurons Revealed byIn VivoImaging with Improved Spatiotemporal Resolution
Authors: Wang Luwei , Nakazawa Shingo , Luo Wenshu , Sato Takuya , Mizuno Hidenobu , Iwasato Takuji 
Published: October 2023

AbstractIndividual neurons in sensory cortices exhibit specific receptive fields based on their dendritic patterns. These dendritic morphologies are established and refined during the neonatal period through activity-dependent plasticity. This process can be visualized using two-photonin vivotime-lapse imaging, but sufficient spatiotemporal resolution is essential. We previously examined dendritic patterning from spiny stellate (SS) neurons, the major type of layer 4 (L4) neurons, in the mouse primary somatosensory cortex (barrel cortex), where mature dendrites display a strong orientation bias toward the barrel center. Longitudinal imaging at 8 h intervals revealed the long-term dynamics by which SS neurons acquire this unique dendritic pattern. However, the spatiotemporal resolution was insufficient to detect the more rapid changes in SS neuron dendrite morphology during the critical neonatal period. In the current study, we imaged neonatal L4 neurons hourly for 8 h and improved the spatial resolution by uniform cell surface labeling. The improved spatiotemporal resolution allowed detection of precise changes in dendrite morphology and revealed aspects of short-term dendritic dynamics unique to the neonatal period. Basal dendrites of barrel cortex L4 neurons were highly dynamic. In particular, both barrel-inner and barrel-outer dendrites (trees and branches) emerged/elongated and disappeared/retracted at similarly high frequencies, suggesting that SS neurons acquire biased dendrite patterns through rapid trial-and-error emergence, elongation, elimination, and retraction of dendritic trees and branches. We also found correlations between morphology and behavior (elongation/retraction) of dendritic tips. Thus, the current study revealed short-term dynamics and related features of cortical neuron dendrites during refinement.
Microglia Are Dispensable for Developmental Dendrite Pruning of Mitral Cells in Mice
Authors: Niiyama Tetsushi , Fujimoto Satoshi , Imai Takeshi 
Published: October 2023

AbstractDuring early development, neurons in the brain often form excess synaptic connections. Later, they strengthen some connections while eliminating others to build functional neuronal circuits. In the olfactory bulb, a mitral cell initially extends multiple dendrites to multiple glomeruli but eventually forms a single primary dendrite through the activity-dependent dendrite pruning process. Recent studies have reported that microglia facilitate synapse pruning during the circuit remodeling in some systems. It has remained unclear whether microglia are involved in the activity-dependent dendrite pruning in the developing brains. Here, we examined whether microglia are required for the developmental dendrite pruning of mitral cells in mice. To deplete microglia in the fetal brain, we treated mice with a colony-stimulating factor 1 receptor (CSF1R) inhibitor, PLX5622, from pregnancy. Microglia were reduced by >90% in mice treated with PLX5622. However, dendrite pruning of mitral cells was not significantly affected. Moreover, we found no significant differences in the number, density, and size of excitatory synapses formed in mitral cell dendrites. We also found no evidence for the role of microglia in the activity-dependent dendrite remodeling of layer 4 (L4) neurons in the barrel cortex. In contrast, the density of excitatory synapses (dendritic spines) in granule cells in the olfactory bulb was significantly increased in mice treated with PLX5622 at postnatal day (P) 6, suggesting a role for the regulation of dendritic spines. Our results indicate that microglia do not play a critical role in activity-dependent dendrite pruning at the neurite level during early postnatal development in mice.
A conserved complex of microneme proteins mediates rhoptry discharge in Toxoplasma
Authors: Valleau Dylan , Sidik Saima M , Godoy Luiz C , Acevedo‐Sánchez Yamilex , Pasaje Charisse Flerida A , Huynh My‐Hang , Carruthers Vern B , Niles Jacquin C , Lourido Sebastian 
Published: October 2023

AbstractApicomplexan parasites discharge specialized organelles called rhoptries upon host cell contact to mediate invasion. The events that drive rhoptry discharge are poorly understood, yet essential to sustain the apicomplexan parasitic life cycle. Rhoptry discharge appears to depend on proteins secreted from another set of organelles called micronemes, which vary in function from allowing host cell binding to facilitation of gliding motility. Here we examine the function of the microneme protein CLAMP, which we previously found to be necessary for Toxoplasma gondii host cell invasion, and demonstrate its essential role in rhoptry discharge. CLAMP forms a distinct complex with two other microneme proteins, the invasion‐associated SPATR, and a previously uncharacterized protein we name CLAMP‐linked invasion protein (CLIP). CLAMP deficiency does not impact parasite adhesion or microneme protein secretion; however, knockdown of any member of the CLAMP complex affects rhoptry discharge. Phylogenetic analysis suggests orthologs of the essential complex components, CLAMP and CLIP, are ubiquitous across apicomplexans. SPATR appears to act as an accessory factor in Toxoplasma, but despite incomplete conservation is also essential for invasion during Plasmodium falciparum blood stages. Together, our results reveal a new protein complex that mediates rhoptry discharge following host‐cell contact.
INFLUENCE OF MARKETING INNOVATIONS ON THE PRODUCT POLICY OF ENTERPRISES
Authors: LYSENKO I. 
Published: October 2023

The purpose. Theoretical and practical aspects of using marketing innovations in the product policy of enterprises have been studied, and their impact on competitiveness and profitability of companies has been justified. The article is to analyze the role of marketing innovations in the product policy of enterprises and their impact on business development in the modern dynamic market environment. Methods. The study is based on relevant scientific research and publications, analyzing the latest trends and approaches in the field of marketing. The research was conducted through the analysis of scientific sources and empirical data, using methods such as dynamic, semantic, comparative analysis, systematization method, grouping method, analysis and synthesis, abstract-logical and expert methods, with the aim of identifying and describing various types of marketing innovations, their peculiarities and relationships with the product policy strategy of the enterprise. The results. Marketing innovations are a key factor in business development in conditions of unstable market environment and changing consumer needs. Their implementation will allow enterprises to ensure sustainable competitive development and positioning in the market. Scientific novelty lies in proposing the implementation of marketing innovations in the practice of enterprises, including the application of new methods of advertising and product promotion, creating exclusive products, using innovative technologies in production, and more. The practical significance of the research lies in its potential to form new theories and concepts in the field of marketing, increase the level of knowledge and qualifications of specialists, consider modern approaches to marketing, such as the development of new products, their market promotion, quality assurance of goods and services, and brand creation and development, with the aim of increasing the competitiveness of enterprises.
Structural transformations and their impact on the mechanical and antifriction properties in the process of alloying graphitized hypereutectoid steel with copper
Authors: Stepanova Natalia V. , Lozhkina Elena A. 
Published: October 2023

The purpose of the work is to develop a cast antifriction material based on an iron-carbon alloy with a high copper content for use in large, heavy duty sliding friction units. Using the casting method in self-hardening liquid glass mixtures, two specimens of hypereutectoid graphitized steel with different copper contents (0.09 and 8.76 wt.%) were produced. To obtain graphite in the steel structure, modification with the silicocalcium (SiCa) was used. The microstructural examination was carried out using optical metallography, SEM and TEM methods. The impact of copper on the structure as well as the mechanical and antifriction properties of graphitized hypereutectoid steel was studied. It was found that adding 8.76 wt.% of copper to the steel composition leads to an increase in the Brinell hardness level of the material from 250 to 300 HB, ultimate tensile strength from 250 to 380 MPa and compressive strength from 1050 to 1200 MPa, which is associated with an increase in the microhardness of pearlite from 350 to 420 HV. To assess the impact of copper on the sliding friction coefficient of graphitized hypereutectoid steel, a curve of sliding friction coefficient vs applied load was plotted; the experiment was carried out according to the liner-on-disk scheme. The wear resistance of materials under sliding friction conditions was also assessed using this method. Copper alloying has a positive effect on the wear resistance of graphitized hypereutectoid steel by increasing the mechanical properties of the material and also by reducing the level of the sliding friction coefficient under boundary lubrication conditions.
Otorhinolaryngological spectrum of diseases leading to Ophthalmological presentation in eye out patient department: An overview
Authors: Singh Divyanshi , Gulati Vibham , Jain Sakshi , Chauhan Sumit Singh 
Published: October 2023

Orbital complications are not so uncommon in ENT diseases. Being an adjacent structure, orbit is commonly involved which can be further complicated by vision loss and intracranial spread of infection and sometimes mortality. In this scenario proper imaging and and newer generation antibiotics can be a game changer. The study aims to demonstrate Ophthalmological manifestations in relation to otorhinolaryngological diseases. This was a retrospective observational study conducted in Eye outpatient department of Regional Hospital, Himachal Pradesh for the period of 1 year from July 2022 to June 2023. In a retrospective investigation, 28 cases of ophthalmological symptoms with root cause of various ENT disorders, mostly paranasal tumour expansions, nasopharyngeal tumour, and nasal furunculosis, were examined in connection to their age, gender, clinical, radiological, and histopathological profile. The most dependable investigating tool was considered as CT scan. Accordingly medical and surgical treatment were adopted owing to location and disease nature. A good cooperation and follow up is needed between ophthalmologist and otolaryngologist to overcome this challenge. A total of 28 patients who met the eligibility criteria were enrolled in the study. Average age of presentation was 36.2 years with 17 males and 11 females. Proptosis was the most common orbital manifestation found in 32.4% cases (n=9). Lagopthalmos was found in 25% cases (n=7) and preseptal cellulitis was found in 14.28% cases (n=4). Orbital cellulitis and cavernous sinus thrombosis was found in 7.14% cases (n=2). Orbital apex syndrome was found in 3.57% cases (n=1). To save the patient from Ophthalmological morbidity and maybe mortality, a high index of suspicion of early orbital involvement by thorough ENT examination is required. A team effort consisting of ophthalmologist and otorhinolaryngologist, acting jointly is needed in order to identify the underlying disease process and provide the appropriate care.
КІБЕРСТАТИСТИКА В УКРАЇНІ. СУЧАСНИЙ СТАН
Authors: Давидюк Андрій , Зубок Віталій , Хохлачова Юлія , Худинцев Микола , Комаров Максим 
Published: October 2023

З розвитком кіберзахисту в Україні збільшуються і технічні спроможності для забезпечення безпеки інформації. Новітнє обладнання здатне збирати великі масиви даних для аналізування потенційних загроз кібербезпеці. Інтеграція аналізу цих даних в процеси кіберзахисту дасть змогу попередити виникнення ряду кіберінцидентів. Водночас існує імовірність того, що суб’єкти забезпечення кібербезпеки під час виконання власних функцій збирають одні і ті ж самі набори даних інформацію, що значно зменшує ефективність їх інформаційного обміну та пов’язаних процесів. З огляду на зазначене, доцільним є впровадження процесів кіберстатистики, що має на меті диференціювати функції суб’єктів забезпечення кібербезпеки за наборами даних, які вони використовують у власній діяльності. Така диференціація допоможе визначити існуючі проблеми в процесах цих суб’єктів та сприятиме впровадженню уніфікованих підходів до збору та аналізування даних в сфері кібербезпеки. Таким чином розробка та впровадження методики збору та оброблення даних кіберстатистики дасть змогу оптимізувати процеси забезпечення кібербезпеки.
АНАЛІЗ ВПЛИВУ ІНОЗЕМНОГО ІТ БІЗНЕСУ НА ЛАНДШАФТ ЗАГРОЗ КІБЕРБЕЗПЕЦІ ДЕРЖАВИ
Authors: Хохлачова Юлія , Давидюк Андрій , Зубок Віталій 
Published: October 2023

Розвиток ІТ індустрії сприяє масштабуванню бізнесу з надання цифрових сервісів. Зростає рівень автоматизації процесів в державних та приватних організаціях. Зростає попит на обчислювальні ресурси. Бізнес в сфері центрів обробки даних починає масштабуватися щоб задовольнити потреби не тільки користувачів однієї країни, а й інших. Водночас користувачі з різних країн світу не обмежені у виборі надавача цих послуг. Вони будують власний ІТ бізнес з використанням ресурсу центрів обробки даних інших держав. Система таких транскордонної взаємодії будується вже багато років і її вплив на внутрішні економічні та соціальні процеси в різних державах зростає також. В наш час доцільно виокремити поняття мережі даних, що можна визначити як сукупність бізнес зв’язків між громадянами різних країн в сфері надання цифрових сервісів та зв’язків центрів обробки даних між собою. На сьогоднішній день відсутній контроль за розвитком мереж даних, зокрема відсутні підходи до вимірювання впливу ризиків кібербезпеки іноземного ІТ бізнесу на економічні та соціальні процеси в державі. Таким мережам притаманний синергетичний розвиток. Війна в Україні показала, що наявність російського ІТ бізнесу сприяла ряду кібератак з метою порушення функціонування об’єктів критичної інфраструктури, державних органів влади та бізнесу. Тому, звісно, не можна заперечувати причетність державних спецслужб до розгортання власної мережі даних для впливу на інші країни. Дана робота присвячена аналізу впливу іноземного ІТ бізнесу на кібербезпеку держави.
Comparison of ultrasound-guided genicular block vs. intrathecal morphine for postoperative analgesia in patients undergoing knee arthroplasty: A randomised prospective study
Authors: Canakci Ebru , Coskun Ilker 
Published: October 2023

Aim: Total knee arthroplasty (TKA) is a major orthopedic surgical procedure often necessitating effective postoperative analgesia. This study aimed to compare the analgesic efficacy of ultrasound-guided genicular block (GB) and intrathecal morphine (ITM) in patients undergoing THA under spinal anesthesia.Method: A prospective, randomized, single-blind study was conducted at Ordu University Training and Research Hospital from October 1, 2022, to April 1, 2023. Eligible patients aged 18 to 90 undergoing knee surgery with spinal anesthesia were randomized into two groups: Group ITM received intrathecal morphine, while Group GB underwent ultrasound-guided triple genicular block. Pain scores, rescue opioid consumption, time to initial mobilization, and Modified Bromage Scale (MBS) scores were assessed at five time points (0, 2, 6, 12, and 24 hours) within the first 24 hours post-surgery.Results: At the 24-hour mark, cumulative tramadol consumption was significantly lower in Group ITM compared to Group GB (35.45±54.84 vs. 63.37±37.7 mg, respectively, p = 0.028). Group ITM also exhibited statistically lower Numeric Rating Scale (NRS) scores at 12 hours (p = 0.005) but had similar scores at other time points. Additionally, time to first ambulation and MBS scores were consistent across all intervals (p < 0.05).Conclusions: In patients undergoing knee arthroplasty with spinal anesthesia, those receiving ultrasound-guided genicular blocks demonstrated higher opioid consumption over a 24-hour period compared to those receiving intrathecal morphine. However, regarding postoperative pain scores, both groups exhibited similar outcomes. These findings provide valuable insights into the selection of postoperative analgesic modalities for TKA patients.
Sistem Pengisian Air Minum Otomatis Peternakan Kambing Berbasis Internet of Things (IoT)
Authors: Saputra Abimanyu Dika , Wardana Dimas Zaeca , Jiddan Abyan 
Published: October 2023

Kebutuhan air merupakan kebutuhan utama bagi kambing untuk terhindar dari dehidrasi. Oleh karena itu, peternak selalu mengawasi ketersediaan air minum bagi kambing tetap tersedia. Akan tetapi, pengawasan secara terus-menerus tidak dapat dilakukan oleh peternak karena peternak perlu mencari pangan, mengirimkan kambing, dan juga istirahat. Pengembangan air minum otomatis dengan bantuan arduino uno sebagai mikrokontroler yang terhubung dengan sensor hc sr04 sebagai yang akan mendeteksi pelampung pada permukaan air sehingga dengan jarak tertentu antara sensor hc sr04 dengan pelampung akan menggerakan servo MG996R. Pada penelitian menguji ketahanan prototype saat digunakan untuk otomatis air. pengujian prototype pada penelitian mengalami beberapa kendala masalah diidentifikasi oleh sebab kesalahan pengembangan prototype yang tidak kaku dan tidak mampunya microSD untuk melakukan penyimpanan secara terus – menerus dalam rentan waktu yang lama.
Operative Fixation of a Humeral Shaft Periprosthetic Fracture After IlluminOss
Authors: Kucharik Michael P. , Andrews Reed , John Mitchell P. , Ohanisian Levonti , Sullivan Alex , Chebli Caroline 
Published: October 2023

Case:
An 82-year-old man sustained a periprosthetic fracture after IlluminOss photodynamic bone stabilization system (PBSS) stabilization for an impending pathologic fracture. Nonoperative management was unsuccessful, and he subsequently underwent operative fixation, which featured lag screw fixation of the spiral distal humeral shaft fracture and osteotomy followed by plate fixation of the pathologic humeral shaft fracture.

Conclusion:
This is the first article to report this complication with IlluminOss PBSS stabilization and details regarding revision surgery.
Physical properties of asteroid Dimorphos as derived from the DART impact
Authors: Raducan Sabina , Jutzi Martin , Cheng Andy , Zhang Yun , Barnouin Olivier , Collins Gareth , Daly Ronald , Davison Thomas , Ernst Carolyn , Farnham Tony , Ferrari Fabio , Hirabayashi Masatoshi , Kumamoto Kathryn , Michel Patrick , Murdoch Naomi , Nakano Ryota , Pajola Maurizio , Rossi Alessandro , Agrusa Harrison , Barbee Brent , Syal M. Bruck , Chabot Nancy , Dotto Elisabetta , Fahnestock Eugene , Hasselmann Pedro , Herreros M.I. , Ivanovski Stavro , Li Jian-Yang , Lucchetti Alice , Luther Robert , Ormö Jens , Owen J. , Pravec Petr , Rivkin Andrew , Robin C. , Sánchez Paul , Tusberti Filippo , Wünnemann Kai , Zinzi Angelo , Epifani Elena Mazzotta , Manzoni C. , May B. 
Published: October 2023

Abstract
On September 26, 2022, NASA's Double Asteroid Redirection Test (DART) mission successfully impacted Dimorphos, the natural satellite of the binary near-Earth asteroid (65803) Didymos. Numerical simulations of the impact provide a means to explore target surface material properties and structures, consistent with the observed momentum deflection efficiency, ejecta cone geometry, and ejected mass. Our simulation that best matches observations indicates that Dimorphos is weak (less than a few Pa), similar to asteroids (162173) Ryugu and (101955) Bennu. We find that a bulk density of Dimorphos, ρB, lower than ≈ 2400 kg/m3, and a low volume fraction of boulders (≲ 40 vol%) on the surface and in the shallow subsurface, are consistent with measured data from the DART experiment. These findings suggest Dimorphos is a rubble pile that might have formed through rotational mass shedding and re-accumulation from Didymos. Our simulations indicate that the DART impact caused global deformation and resurfacing of Dimorphos. ESA's upcoming Hera mission may find a re-shaped asteroid, rather than a well-defined crater.
A Comprehensive Study of the Estuary Sea Environment in the Bay of Bengal, near the Mahanadi River Confluence
Authors: Pati Sharada Shrinivas , Nayak Sumitra , Mishra Sangeeta , Panda Bhabani Shankar , Mahala Suravi Susmita , Mohanty Sarat Kumar , Behera Rajaram , Mallick Niranjan , Murugesan Kailasam 
Published: October 2023

Abstract
This study focuses on the environmental conditions of the Mahanadi Estuary near Paradeep Harbor and the adjacent sea. Data collected from May 2013 to April 2020 from 32 GPS fixed stations was analyzed to assess the water quality in different zones (estuarine, mixed zone, mixed zone south, and mixed zone north) of study area. Parameters such as pH, SST, TSS, nitrite, phosphate, silicate, TOC, chlorophyll, fecal coliform, and heavy metals were used to estimate the Water Quality Index (WQI) for each zone. The study found a deterioration (>30%)in the overall water quality of the Mahanadi Estuary from 2013 to 2020, potentially attributed to river inflows, port activities, and industrial outflows in to the coastal ecosystem. Seasonal variations in temperature, salinity, turbidity, nitrite, nitrate, and ammonia were observed. The water quality showed a deteriorating trend in estuarine, mixed zone, mixed zone south, and mixed zone north. Based on the water quality indices, the ecosystem shows moderate levels of stress. The degraded water quality highlights the need for a targeted mitigation plan to reduce external pressures and enhance the overall ecosystem quality.
Ruthenate perovskite with face-sharing motifs for sustainable alkaline hydrogen evolution
Authors: Tong David Shuk-Yin , Zhu Chuanhui , Tian Hao , Tan Pengfei , Huang Bin , Zhao Shuang , Cai Guo-Hong , Yuan Chongyang , Zhao Mei-Huan , Cao Meng , Zhao Jianfa , Shi Luchuan , Song Haili , Huang Keke , Feng Shouhua , Croft Mark , Jin Chang-Qing , Li Man-Rong 
Published: October 2023

Abstract
Hydrogen-centered electrochemical technologies play a vital role in sustainable energy conversion and storage. One of the challenges in achieving cheap hydrogen is to bridge the gap between advanced electrocatalysts and highly effective electrodes. Here, we synthesized BaRuO3 in four polymorphs with distinct RuO6 connections to rationalize the impact of crystal structure feature on transferable alkaline hydrogen evolution activity. The 9R-BaRuO3 displays the best hydrogen evolution reaction activity and stability in alkaline solution with a small Tafel slope of 30 mV dec-1 and a low overpotential of η10 < 51 mV, which is comparable to Pt/C and superior to the most highly efficient ruthenium-based oxide hydrogen evolution catalysts reported to date. Such performance is ascribed to its high intrinsic activity that is delivered by the d-d interaction and optimal intermediate adsorption. More importantly, 9R-BaRuO3 can be easily synthesized on a large scale as demonstrated by the 9R-BaRuO3-500 g sample in this study. The high intrinsic activity and micron size of 9R-BaRuO3 powders enable a successful performance transfer from a lab-based three-electrode system to a commercial alkaline electrolyser operated under industrial conditions. The findings presented here not only demonstrate a robust and efficient HER electrocatalyst for practical electrolysers, but also emphasize the effect of structural features in the design of advanced electrocatalysts.