bluuebunny/crossref_metadata_2025_split huggingface dataset:
Winding up to wind down: designing the end of one’s medical career with intentionality
Authors: Stoller James K , Rabalais Gerard 
Published: January 2025

BackgroundPhysicians are planful beings, spending much time imagining and trying to design their ideal careers. Yet, despite actuarial expectations that physicians may spend almost two decades in retirement, it is paradoxical that little attention is generally given to designing an ideal retirement.MethodsThe coauthors are physicians in the latter parts of their careers who both teach leadership to physicians and have thought deeply about physician transitions, including retirement. This perspective narrative is based on their observations, actual experience of colleagues who have retired well and their review of relevant literature.ResultsSeveral forces restrain physicians from imagining an ideal retirement: first, as with people in general, physicians may be averse to change, and second, thinking about retirement may be framed through a deficit-based lens (eg, “I am retiring because I want to avoid some unpleasant features of my current career.”) Though physicians are predisposed to deficit-based thinking by virtue of their longstanding practice of developing differential diagnoses in clinical reasoning, appreciative or asset-based thinking about retirement (eg, “I am retiring because I am attracted to activities that have not otherwise been possible in my career.”) offers richer possibilities and is encouraged. A rubric for navigating retirement regards being intentional about identity, relationships and purpose in the new state. A taxonomy for types in retirement is offered, recognising that physicians may exemplify more than one type or migrate among them. Finally, several examples of successful retirement by the authors’ colleagues are offered.ConclusionsWe endorse physicians’ being as planful and intentional about retirement as they are about designing their careers. Our hope is that the described issues and approach enhance physicians’ likelihood to achieve the fulfilment and impact in retirement that they seek.
Compatibility of Commercial Fungicide Formulations with Plant-Associated Methylobacterium
Authors: Palberg Daniel , Emery Neil RJ 
Published: January 2025

Symbiotic Methylobacterium comprise a significant part of the plant microbiome and are known to benefit host plant growth, development, tolerance to abiotic stress, and enhanced disease resistance. The wide application of commercial broad-spectrum fungicide formulations in contemporary agriculture practices has necessitated the investigation of compatibility between popular pesticide products and bacterial endophytes, especially as the Methylobacterium are increasingly considered for agronomic use including biocontrol of phytopathogens. This study provides an evaluation of compatibility between a extensive inventory of 40 Methylobacterium strains in response to commercial pesticide formulations, each containing different agtive ingredients: DYNASTY® and QUADRIS® (azoxystrobin), MAXIM®480 (fludioxonil), and APRON XL® LS (metalaxyl-M). Using a diffusion disk assay, no sensitivity of tested Methylobacterium strains could be detected against any fungicide product, at doses within and above the recommended therapeutic window (1-100 µg). Potency of formulations across the same range were confirmed using the sensitive phytopathogen Fusarium graminearum. As Methylobacterium spp. continue to emerge as suitable candidates for various roles in biotechnology including agriculture, a better understanding on the compatibility between this important genus and commercial fungicide products has become a relevant consideration for integrated pest management practices.
Sequence-based identification of polyamine oxidase genes in Sorghum bicolor L.
Authors: Ebeed Heba T. , El-Zonkorany Mohamed , Habib Eman , Ali Esraa , Zahran Ahmed , Ragab Aya Hamdy 
Published: January 2025

Polyamine oxidases (PAOs) are enzymes associated with polyamine catabolism and play important roles in growth and development and stress tolerance of plants. In the present study, genome-wide discovery and analysis of the PAO family in sorghum was done utilizing model PAO of Arabidopsis. Six PAO genes were found using publicly available genomic data. Sorghum has the PAO gene representatives distributed throughout four chromosomes (chr1, chr3, chr6, and chr7), and most members have eight to nine exons. The molecular weights of PAO proteins range from 53 to 63 kDa. PAO proteins have a theoretical PI between 5.2 and 8.1. The identification and characterization of PAO gene members in sorghum lay the foundation for further experimental studies elucidating their roles in growth, development, and stress responses, ultimately contributing to our understanding of plant biology, with significant implications for plant breeding by providing valuable insights into potential targets for enhancing stress tolerance and improving crop performance.
Phenomenology of the dark matter sector in the 2HDM extended with complex scalar singlet
Authors: Dutta Juhi , Moortgat-Pick Gudrid , Schreiber Merle 
Published: January 2025

Abstract
The two-Higgs-doublet model augmented with a complex scalar singlet (2HDMS) is a well-motivated candidate for Beyond Standard Model (BSM) Physics. We investigate the dark matter phenomenology of the 2HDMS with the complex scalar singlet as the dark matter candidate. We perform a study of the parameter space allowed by existing theoretical and experimental constraints from dark matter, flavour physics and collider searches. Further, we discuss a few benchmark scenarios to test the discovery potential for the 2HDMS at the HL-LHC and at future high-energy

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+

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colliders.
Report on ReAnimate'24: 2024 Summer School on Retro Gaming History, Critic, and Development
Authors: Guéhéneuc Yann-Gaël , Ullmann Gabriel C. , Politowski Cristiano , Petrillo Fabio , Therrien Carl 
Published: January 2025

For many years, there has been an interest in ''old'' games, either real old games or recent games with an ''old'' look and feel. The retro gaming community has grown from very niche to mainstream, following the general gaming trend. Retro gaming has also entered the general psyche with books, movies, documentaries, articles, etc. becoming mainstream. However, despite this mainstream status and some recent books, retro gaming remains under-studied in academia and existing research rarely enters mass media.
We proposed a summer school dedicated to retro gaming, which invited both the humanities and engineering fields to provide unique insights on retro gaming, both theoretical and practical, and opportunities for cross-fertilization among research fields.
This summer school welcomed anyone interested in retro gaming. In particular, students in the humanities learned about general game development and the particularities of retro games while students in engineering learned about the history of gaming and theories about games and game design.
This summer school, supported by the ACM SIGSOFT and Cloanto, featured lectures in the mornings and practical, hands-on sessions in the afternoon given by experts on (retro) games as well as site visits, panels, and discussions to foster exchanges, create a community, and promote the studies of retro games.
Assessment of the Association Between Neuraxial Anesthesia and Back Pain After Delivery: A Systematic Review and Meta‐Analysis
Authors: Timerga Sara , Walle Getaw , Mebratu Wondwosen , Befkadu Aynalem 
Published: January 2025

Background: Back pain after delivery both under cesarean section and spontaneous vaginal delivery is the most common pregnancy‐related musculoskeletal problem. There are multiple studies that emphasize the effect of epidural anesthesia and spinal anesthesia on the incidence and severity of postdelivery back pain. There are others stating no association between the two.Objective: The aim of this study is to summarize the relationship between back pain after delivery and neuraxial anesthesia.Methods: Studies identified from database: Cochrane Library, The Virtual Health Library, National Library of Medicine PubMed, Google Scholar, and citation searching with both experimental and observational study design were included. Exposed and nonexposed incidence of back pain was extracted to analyze the pooled odds ratio assessing the association of postpartum back pain and neuraxial anesthesia. Heterogeneity was checked across studies using Cochrane Q test statistic and I2. Small study effect was assessed using a funnel plot graphically and nonparametric rank correlation (Begg) test.Results: Four RCT and 11 observational studies were identified for analysis. The studies included mothers delivering under cesarean section and vaginal delivery with epidural anesthesia, spinal anesthesia, and combined spinal epidural anesthesia. Based on the 15 studies included in this meta‐analysis, the pooled odds ratio according to random effect restricted maximum—likelihood model was 1.2 (95% CI (0.77–1.86)) with p value = 0.43. There was a significant heterogeneity with I2 = 97.76%, H2 = 44.58, and Cochrane Q statistics p value = 0.001.Conclusion: Our result suggests neuraxial anesthesia may not be the cause or the risk factor for the overwhelmingly high incidence of back pain women experience after delivery.
A Six Years’ Trend Analysis of Antimicrobial Resistance Among Bacterial Isolates at Public Health Institute in Amhara Region, Ethiopia
Authors: Abejew Asrat Agalu , Fenta Teferi Gedif , Wubetu Gizachew Yismaw 
Published: January 2025

Background: Antimicrobial resistance (AMR) is one of the top global threats to public health. This study determined trends in AMR from 2016 to 2021 in a regional research laboratory in Northwest Ethiopia.Methods: Data from 2016 to 2021 was extracted from a database. Bacterial identification and resistance tests were made using the standard microbiologic procedures. The results were described, trends in AMR were determined using polynomial regressions, and binary logistic regression at p value < 0.05 was used.Results: From 2016 to 2021, 25,143 specimens were sent for culture and susceptibility testing, among which 16,825 (66.9%) bacteria were isolated. About 12,528 (74.5%) isolates were gram‐negative, and 4297 (25.5%) were gram‐positive. Klebsiella pneumoniae (3783, 30.2%) and Escherichia coli (3199, 25.5%) were the most common gram‐negative bacteria, whereas coagulase‐negative Staphylococcus spp. (CoNS) (1765, 40.1%) and Staphylococcus aureus (1293, 30.1%) were the most common gram‐positive bacteria. The overall prevalence of AMR was 2738 (59.9%), of which about 1807 (66.0%) accounted for gram‐negative and 931 (34.0%) for gram‐positive bacteria. K. pneumoniae (743, 80.2%), Enterobacter cloacae (196, 74.8%), and Acinetobacter baumannii (213, 66.6%) were the most common resistant isolates among gram‐negative bacteria, while CoNS (406, 58%), Streptococcus species (34, 50%), and S. aureus (196, 37%) were from gram‐positive bacteria. About 571 (20.9%) of bacteria were resistant to 2–10 drugs. The overall trend of AMR has been rising from year to year, reaching a peak in 2019 which was approximately 66% and then after has been predicted to decline.Conclusion: AMR in the regional laboratory is prevalent and has been increasing although the quadratic equation has revealed downward‐opening parabola over time. A growing number of multidrug‐resistant bacteria are an alarm to awaken policymakers and those concerned to intervene before it is too late. This calls for a periodic, integrated, and continuing system to monitor AMR for commonly used antibiotics.
Indoor Residual Spraying With Fludora Fusion for Malaria Control in Pyrethroid‐Resistant Areas of Gujarat, India: A Community‐Randomized Trial
Authors: Kamaraju Raghavendra , Pant Chandra Sekhar , Uragayala Sreehari , Baharia Rajendra Kumar , Srivastava Harish Chandra , Yadav Rajpal Singh 
Published: January 2025

Background: The development of insecticide resistance in malaria vectors has necessitated a need to evaluate new insecticide molecules with different modes of action. In the present study, Fludora Fusion 562.5 WP‐SB (clothianidin 50% + deltamethrin 6.25% AI/kg) was evaluated for its efficacy and residual action for the control of pyrethroid‐resistant malaria vector, Anopheles culicifacies (Diptera: Culicidae), during May 2017 to February 2018 in Gujarat state, India.Methods: Fludora Fusion at the dose of 225 mg AI/m2 and bendiocarb at a dose of 400 mg AI/m2 as a positive control were sprayed in 5 villages each in districts of Kheda, Vadodara, and Panchmahal. The persistence of their efficacy on different local surfaces was determined against An. culicifacies. Entomological indices such as indoor resting density, human landing collections, pyrethrum spray collections, and exit trap collections were monitored to assess the impact of spraying.Results: The observed residual action of Fludora Fusion on mud and cement surfaces was for 6 months and bendiocarb for 3–4 months on both surfaces. Indoor resting densities and parous rate of An. culicifacies were significantly lower in houses sprayed with Fludora Fusion compared to bendiocarb‐sprayed houses. Daily entomological inoculation rate (EIR) declined from 1.275 during prespray period to 0.5225 in the Fludora Fusion arm and 0.3802 in the Ficam arm in postspray period, indicating a reduction in the malaria transmission potential of An. culicifacies in both arms.Conclusion: Based on the residual action of the Fludora Fusion on most common sprayed surfaces and its effects on the elements of vectorial capacity, Fludora Fusion at 225 mg/m2 dose was found effective for more than 6 months and could be a potential option for the control of resistant mosquito vectors.
The Antiaging and Antioxidative Effects of a Combination of Resveratrol and High‐Intensity Interval Training on the Frontal Lobe in Aged Rats: The Role of SIRTS 4, SIRTS 5, SOD1, and SOD2
Authors: Mehrabi Amin , Nuori Reza , Gaeini Abbasali , Amirazodi Maryam , Mehrtash Mohammad , Esfahlani Mohsen Abedini , Bahrami Mina , Bejeshk Mohammad Abbas , Rajizadeh Mohammad Amin 
Published: January 2025

Introduction: High‐intensity interval training (HIIT) is a form of interval exercise that enhances capacity and benefits well‐being. Resveratrol is a naturally occurring polyphenol prevalent in grapes and red wine, demonstrating significant health effects on the body. This study sought to evaluate the synergistic effects of swimming HIIT and resveratrol intake on the expression of SIRTs 4, SIRTs 5, and superoxide dismutases (SOD1 and SOD2) in the frontal lobe of elderly rats.Materials and Methods: Forty‐five male Wistar rats, aged 22 months, were categorized into five groups: the control group (CTL), the swimming HIIT group (Ex: Exercise), the swimming HIIT with resveratrol group (R + Ex), the resveratrol group (R), and the solvent control group (vehicle). The R + Ex group engaged in high‐intensity interval swimming and ingested resveratrol (10 mg/kg/day via gavage) for 6 weeks. During the initial and final sessions of each week, blood samples from the rats in the Ex and R + Ex groups were collected for lactate analysis. The proteins SIRTs 4 and 5, as well as SODs 1 and 2, were quantified using the western blot approach.Results: Integrating HIIT with resveratrol markedly enhanced the expression of SIRT4, SIRT5, and antioxidant enzymes in the frontal lobe of elderly rats.Conclusion: Resveratrol and HIIT, particularly their synergistic effects, provide antioxidant and antiaging benefits on the frontal lobe of aged rats.
Patient-Derived Organoids and Xenografts Uncover Therapeutic Vulnerabilities in Colorectal Signet Ring Cell Carcinomas
Authors: Chaudhary Nazia , La Ferlita Alessandro , Choudhary Bhagya Shree , Jog Eeshrita , Kazi Mufaddal , Yahya Showket , Dalwai Afiya , Ostwal Vikas , Singh Satishkumar , Redkar Siddhi , Khapare Nileema , Kailaje Vaishali , B Akshaya , Gera Poonam , Bal Munita , Verma Nandini , Thorat Rahul , Saklani Avanish , Sehgal Lalit , Dalal Sorab N. 
Published: January 2025

Abstract

Purpose:
Identifying therapeutic targets for signet ring cell carcinoma (SRCC) of the colon and rectum is a clinical challenge because of the lack of patient-derived organoids (PDO) or patient-derived xenografts (PDX). To address this unmet need, we present a robust method for establishing PDO and PDX models. We demonstrate that these models identify novel therapeutic strategies targeting therapy resistance and peritoneal metastasis.

Experimental Design:
We derived nine PDO and PDX models from patients with colorectal SRCC. Detailed histopathologic characterization confirmed the fidelity of these models to the original tumors. Drug sensitivity assays were conducted in vitro and in vivo to assess the therapeutic efficacy and impact on peritoneal metastasis. An RNA sequencing analysis was performed to identify critical pathways contributing to therapy resistance and metastatic progression.

Results:
We successfully developed and characterized PDO and PDX models from nine patients with SRCC. The SRCC PDO and PDX models exhibited histopathologic features consistent with those of the original tumors, including high mucin content and eccentric nuclei. They demonstrated increased sensitivity to FOLFIRI combined with paclitaxel or vincristine, reducing peritoneal metastasis. RNA sequencing analysis revealed the upregulation of autophagy genes in SRCC. Treatment with chloroquine alone resulted in decreased tumor growth and peritoneal metastasis.

Conclusions:
Our study establishes PDO and PDX models as robust platforms for studying SRCC and identifying potential therapeutic strategies. Combining FOLFIRI with paclitaxel/vincristine or chloroquine alone inhibits tumor growth and prevents peritoneal metastasis, showing promise for clinical translation. These findings suggest that combining FOLFIRI with intraperitoneal paclitaxel warrants further investigation in phase I clinical trials for patients with SRCC.
Targeting AXL inhibits the growth and metastasis of prostate cancer in bone
Authors: Chiu Chun-Lung , Zhang Dalin , Zhao Hongjuan , Wei Yi , Polasko Alexandra Lapat. , Thomsen Mikkel Thy. , Yang Vanessa , Yang Kasie Kexin. , Hauck Spencer , Peterson Eric E. , Wen Ru M. , Qiu Zhengyuan , Corey Eva , Miao Yu Rebecca , Rankin Erinn B. , Peehl Donna M. , Huang Jiaoti , Giaccia Amato J. , Brooks James D. 
Published: January 2025

Abstract
Purpose: After failing primary and secondary hormonal therapy, castration-resistant and neuroendocrine prostate cancer metastatic to the bone is invariably lethal, although treatment with docetaxel and carboplatin can modestly improve survival. Therefore, agents targeting biologically relevant pathways in PCa and potentially synergizing with docetaxel and carboplatin in inhibiting bone metastasis growth are urgently needed. Experimental Design: Phosphorylated (activated) AXL expression in human prostate cancer bone metastases was assessed by immunohistochemical staining. We evaluated the effects of a novel soluble AXL signaling inhibitor, sAXL (batiraxcept or AVB-S6-500), on the tumor growth and lung metastases in PCa patient-derived xenograft models (PDX) that implanted intratibally. After injection of LuCaP cells into the tibiae, tumors were treated with batiraxcept and docetaxel or carboplatin alone or in combination, and tumor growth was monitored by serum PSA or bioluminescence. Tumor burden was quantified by human-specific Ku70 staining, and metastasis to the lung was determined using qPCR. Transcriptomic profiling, western blotting and immunohistochemistry were performed to identify treatment-regulated gene and protein profile changes. Results: High AXL phosphorylation in human PCa bone metastases correlated with shortened survival. Batiraxcept alone or in combination with docetaxel or carboplatin significantly suppressed intratibial tumor growth and suppressed metastasis to the lung through multiple mechanisms, including repression of cancer stemness genes (CD44, ALDH1A1, TACSTD2, ATXN1) and the PI3K, JAK, MAPK, and E2F1/NUSAP1 signaling pathways. Conclusions: Our study provides a robust preclinical rationale and mechanisms of action for using batiraxcept as a single agent or in combination with docetaxel or carboplatin to treat lethal mPCa.
Daoist Philosophies Past, Present and Future: Curing the Platypus Syndrome
Authors: Wawrytko Sandra A. 
Published: January 2025

Abstract
If Chinese Philosophy is to be viewed as Contemporary Philosophy, we must address the explicit and implicit biases our philosophical colleagues harbor about “legitimate” philosophy. An apt analogy involves the challenge posed to the European taxonomy of species by the “discovery” of the platypus. Now recognized as a distinct species of mammal (monotreme), the platypus was initially denounced as a hoax, then grudgingly accepted as a “primitive” evolutionary dead end. Using Daoist philosophy as an example, this essay offers a pedagogical strategy for shifting from the Fixed Mind’s adulation of “the [Western] Canon” to the Growth Mind receptive to Daoism’s philosophical resources.
Exploring the techno-functional potential of Arsenura armida and Acheta domesticus flours in wheat bread formulation
Authors: Cortazar-Moya S. , Hernández-Figueroa R.H. , Vera-Santander V.E. , López-Malo A. , Morales-Camacho J.I. 
Published: January 2025

Abstract
Food fortification and enrichment are popular trends in food production, aiming to boost nutritional value, health benefits, and techno-functional properties. These objectives can only be achieved if fortified foods are consumed widely and in sufficient quantities. Therefore, bakery products such as bread or pasta are often chosen as food vehicles. This study investigated the impact of adding insect meal to wheat bread, focusing on quality, physical, and sensory properties. The aim was to partially replace wheat flour with flours from Arsenura armida or Acheta domesticus, testing each independently at 5 parts and 10 parts substitution levels. A factorial design (2̂3) was used to assess the effects of the selected proportions of insect meal, wheat flour, and water on the bread characteristics.
Breads with A. armida meal did not show significant differences in moisture content, water activity (aw), and width-to-height ratio (W/H) compared to the control (). In contrast, bread with A. domesticus meal showed significant differences in aw, W/H, and moisture (), with the sample substituted by 10 g of A. domesticus meal showing the lowest moisture (24.5%) and highest W/H (5.43). Color analysis revealed no significant difference () in the total color difference (ΔE) of the crumb of all the formulations tested, neither for A. armida nor A. domesticus. The evaluated proportions of insect meal did not affect this characteristic. The sensory evaluation (9-point hedonic scale) showed no significant differences () in aroma, color, crust and crumb appearance, flavor, and overall acceptability among formulations with A. armida or A. domesticus flours compared to the control. Scores ranged from 5.5 ± 2.4 to 6.9 ± 2.1, corresponding to “like slightly.” The factorial design identified formulations with improved texture, which is associated with the general acceptability of insect flours (A. armida and A. domesticus) when mixed with wheat flour and water to produce bread.