bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cells promote ovarian reconstruction in mice
Authors: Li Jiazhao , Fan Haonan , Liu Wei , Zhang Jing , Xiao Yue , Peng Yue , Yang Weijie , Liu Wenwen , He Yuanlin , Qin Lianju , Li Jing 
Published: October 2023

Abstract
Background: Studies have shown that chemotherapy and radiotherapy can cause premature ovarian failure and loss of fertility in female cancer patients. Ovarian cortex cryopreservation is a good choice to preserve female fertility before cancer treatment. Following the remission of the disease, the thawed ovarian tissue could be transplanted back and restore fertility of the patient. However, there is a risk to reintroduce cancer cells in the body and leads to the recurrence of cancer. Since current in vitro culture techniques of primordial follicles have a low success rate in obtaining mature oocytes, an artificial ovary with primordial follicles may be good way to solve this problem.
Methods: In the study, we established an artificial ovary model based on the participation of mesenchymal stem cells (MSCs) to evaluate the effect of MSCs on follicular development and oocyte maturation. P2.5 mouse ovaries were digested into single cell suspensions and mixed with bone marrow derived mesenchymal stem cells (BM-MSCs) at a 1:1 ratio and a reconstituted ovarian model was generated by using phytohemagglutin. The phenotype and mechanism studies were explored by follicle counting, immunohistochemistry, immunofluorescence, in vitro maturation (IVM), in vitro fertilization (IVF), real-time quantitative polymerase chain reaction (RT-qPCR), and Terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL) assay.
Results: Our results revealed the addition of BM-MSCs in reconstituted ovary could mesenchymal stem cells at the single-cell level reveals that the addition of stem cells increase the survival rate of oocytes and promote the follicular growth and development. The reconstituted ovaries have normal folliculogenesis and oocyte maturation. After aggregation with ovarian cells, BM-MSCs didn’t pr oliferate and participate in the formation of follicles. On the contrary, their numbers decreased significantly along with the development of reconstituted ovary and the cells were found to be surrounded with growing follicles. When theca cells were labeled with CYP17a1, only individual theca cells showed the overlapped staining with GFP labeled BM-MSCs. The results suggest that BM-MSCs may participate in directing the differentiation of theca layer in the reconstituted ovary.
Conclusions: BM-MSCs promoted ovarian cell survival, follicle formation and development in the artificial ovary. Since it didn’t proliferate in the reconstituted ovary, it may represent a new and safe approach on clinical fertility preservation by improving the success rate of thawed ovaries transplantation.
Chitosan-insulin nano-formulations as critical modulators of inflammatory cytokines and Nrf-2 pathway to accelerate burn wound healing
Authors: Sharda Deepinder , Ghosh Sandip , Kaur Pawandeep , Basu Biswarup , Choudhury Diptiman 
Published: October 2023

Abstract
Burn injuries are characterized by prolonged inflammatory phases, neurovascular damage, and hypermetabolism, eventually causing improper tissue regeneration. Insulin has gained considerable attention in normal and diabetic wound healing, yet its role in burn wounds remains poorly understood. In this study, insulin-chitosan nano-formulations (ICNP) were synthesized using a simple and robust mechanism and characterized to monitor specific interactions between insulin and chitosan, and the particles measuring approximately 30 nm in size exhibited mild alterations in the amide I, II, and III bonds of the insulin protein along with impressive insulin loading efficiency of 88.725 ± 0.295% under physiological conditions, and significantly improved burn wound healing in vitro (HEKa cells) and in vivo (murine third-degree burn model). The underlying mechanism behind superior wound closure and tissue remodeling was attributed to significant early phase reduction of pro-inflammatory cytokine IL-6 levels in ICNP-treated mice, while anti-inflammatory cytokine IL-10 levels became markedly elevated, resulting in enhanced re-epithelialization and collagen deposition. Furthermore, treatment of ICNP was associated with unregulated expression of Nrf-2, a key regulator of oxidative stress and inflammation, indicating their molecular crosstalk. These findings highlight the potential of ICNP as a promising therapeutic formulation for burn wound healing, promoting wound closure by modulating inflammatory phases, making it a valuable candidate for further clinical development in burn care.
Another emerging contaminant in the sinking city: The first evidence of metformin detected in Jakarta waters
Authors: Koagouw Wulan , Simanjuntak Erna , Hazell Richard J. , Subandi Riyana , Ciocan Corina 
Published: October 2023

Abstract

Pharmaceutically active compounds have been considered contaminants of emerging concern, in response to evidence that these substances may adversely affect non target organisms. The pharmaceutical metformin is the most commonly prescribed anti-diabetes medicine throughout the world. Metformin has been detected in numerous freshwater systems as well as in seawater at a number of sites around the world over the last few years, but has never been reported in the Indonesian capital city Jakarta. Several recent studies have highlighted various ecotoxicological effects of this medicine on aquatic organisms. Here we report the first evidence of metformin’s presence in Jakarta waters. Samples from the Angke river, one of the main rivers in Jakarta, were collected from six sites. Metformin was detected at three sites in concentrations ranging from 27 ng/L to 414 ng/L. Metformin is one of the most detected APIs (active pharmaceutical ingredients) in aquatic environments worldwide, and there is increasing concern regarding its impact on the health of wildlife and humans. However, this is the first report of metformin contamination in Jakarta waters, adding to the evidence of potentially increased pollution with pharmaceuticals, as noted in our previous studies. With no natural degradation processes, these chemical compounds can be easily reintroduced to the food chain and impact human health.
Stark effect and orbitals hybridization of moir\'e interlayer exciton in $ MoSe{2}/ WSe{2} $ heterobilayer.
Authors: Hannachi Houssem Eddine , Jaziri Sihem 
Published: October 2023

Abstract
We theoretically investigate the interlayer excitons response in the $WSe_{2}/MoSe_{2}$ heterobilayer under the effects of in-plane and out-of-plane static electric fields. We thoroughly analyze a wide range of properties pertaining to the interlayer exciton, including the binding energy, Stark shift, orbital hybridization, photoluminescence (PL) spectrum, and radiative lifetime. We examine various factors influencing interlayer exciton behavior, including the dielectric environment and the moir\'e traps effects.
Our results demonstrate that the in-plane electric field significantly affects the binding energies of the interlayer exciton, leading to energy splitting between states with non-zero angular momentum, such as the $2p_{\pm}$ dark states. Furthermore, we show that the applied in-plane electric field results in orbitals hybridization of the interlayer exciton, including hybrid states such as $1s$, $2p_{\pm}$, and $2s$.
In counterpart, we demonstrate that an out-of-plane electric field induced by a double-gate setup causes a quadratic Stark effect on the center of mass eigenenergies of the interlayer exciton and leads to energy splitting of degenerate states, result in an orbitals hybridization of center of mass eigenvectors. Finally, we determine the PL spectra and radiative lifetime of the moiré interlayer exciton as a function of electric field strength. Our results reveal that the $2p_{\pm}$ dark state becomes brighter through a one-photon PL process as the in-plane electric field strength increases. This phenomenon is directly attributed to the coupling between the $ns$ and $ np_{\pm} $ states generated by the in-plane electric field.
In short, our investigation can help experimenters to designing novel optoelectronic applications, such as on-chip electro-optic modulators and TeraHertz devices.
Red blood cell folate level is associated with periodontitis in American adults: results from the NHANES 2009-2014
Authors: Liu Zefei , Luo Shiyi , Jiao Ruofeng , Li Wei , Jin Fuqian , Sun Jiangling , Ma Shu , Song Jukun , Chen Zhu 
Published: October 2023

Abstract

Background
The relationship between folate and periodontal disease has been previously reported. Red blood cell (RBC) folate is an indicator of long-term folate nutrition. The aim of this study was to use the NHANES database to determine whether red blood cell folate is associated with moderate/severe periodontitis.
Methods
A cross-sectional analysis of 10,168 participants in the NHANES database from 2009–2014 was performed. Multivariate logistic regression was used to determine the OR and 95% CI for periodontitis in each group. Restriction cube spline model was used for analyzing dose-response relationship. Finally, subgroup analysis was performed to test whether the results were robust in different populations.
Results
After adjusting for confounding factors, there was an inverse correlation between RBC folate concentration and moderate/severe periodontitis. The lowest fraction Q1 (< 360ng/mL) of RBC folate concentration was used as the reference group, multivariable-adjusted ORs and 95% CIs of the second (360-463ng/mL), third (464-569ng/mL), fourth (570–732 ng/mL), and the highest quintile (> 733 ng/mL) categories were 0.87 (0.76–0.99), 0.81(0.70–0.93),0.75(0.65–0.88),0.62(0.53–0.73) respectively (all p < 0.05).
Conclusion
In representative samples of American adults, high red blood cell folate was at low risk for moderate/severe periodontitis. At the same time, dentists and policymakers should pay attention to the harm caused by high red blood cell folate level.
HOOK3 suppresses proliferation and metastasis in gastric cancer via the SP1/VEGFA axis
Authors: Shi Tongguo , Yang Kexi , Li Juntao , Zhu Jinghan , Chen Yuqi , He Yuxin , Wang Jiayu , Shen Kanger , Wang Kun , Chen Weichang 
Published: October 2023

Abstract
HOOK3, a member of the human hook microtubule-tethering protein family, has been implicated in the progression of cancer. However, the role of HOOK3 in the pathogenesis of gastric cancer (GC) remains incompletely understood. In this study, we investigated the expression of HOOK3 protein in GC tissues using immunohistochemistry (IHC). The findings of our study indicate that the expression levels of HOOK3 in GC tissues were relatively low. Furthermore, a significant negative association was seen between HOOK3 expression and the prognosis of patients with GC. The suppression of HOOK3 resulted in a notable increase in the proliferation, migration, and invasion of GC cells. Conversely, the overexpression of HOOK3 had the opposite impact, reducing these cellular processes. Moreover, in vivo tests have shown evidence that the overexpression of HOOK3 significantly inhibited the formation of tumors and the spread of GC cells to the lungs. In a mechanistic manner, the analysis of RNA-seq data demonstrated that the knockdown of HOOK3 resulted in a notable increase in the expression of VEGFA in GC cells. Furthermore, the upregulation of VEGFA counteracted the impacts of HOOK3 upregulation on the proliferation, migration, and invasion of GC cells. Furthermore, it was revealed that SP1 exhibited the ability to bind to the promoter region of VEGFA. Moreover, the overexpression of SP1 successfully counteracted the inhibitory impact of HOOK3 overexpression on the expression of VEGFA in GC cells. In summary, the results of our study indicate that HOOK3 has a role in inhibiting the growth, migration, and invasion of GC cells by modulating the SP1/VEGFA pathway. These findings contribute significant knowledge to our understanding of the underlying mechanisms involved in the development of GC.
Genome-wide identification and characterization of Aux/IAA gene family in grape and functional identification of VaIAA3 in response to cold stress
Authors: Li Min , Liang Guoping , Cheng Yongjuan , Gou Huimin , Che Lili , Lu Shixiong , Mao Juan 
Published: October 2023

Abstract
The Aux/IAA gene is a key gene that regulates auxin signal transduction in plants. Although IAA genes have been characterized in various plant species, the role of the IAA gene in grape cold resistance is unclear. In order to further explore the members of Aux/IAA gene family in grape and their functions, in this study, based on the genomic data of ‘Pinot Noir’ (Vitis vinefera cv. ‘Pinot Noir’) and Shanputao (Vitis amurensis), 25 VvIAA genes and 18 VaIAA genes were identified. The VaIAA genes exhibited different expression patterns under diferent temperature periods by qPCR. VaIAA3 was selected as a candidate gene for further functional analysis because of its high expression level under low temperature stress. The subcellular localization showed that VaIAA3 was localized in the nucleus. Additionally, under 4°C treatment at 24 h, the relative expression level of VaIAA3 and antioxidant enzyme activity of the three transgenic lines (OE-1, OE-2, OE-3) were significantly increased, while the contents of relative electrolytic conductivity (REC), malondialdehyde (MDA) and hydrogen peroxide (H2O2) were lower under cold stress. Taken together, these results provide some possibilities to study the identification and function of Aux/IAA gene families in different grape varieties. Importantly, heterologous over-expression of VaIAA3 can enhance resistance of Arabidopsis thaliana under cold stress, which provides a theoretical basis for further use of IAA3 gene to improve grape agronomic traits.
Quantum Magnifying Glass for Chemistry at the Nanoscale
Authors: Reiher Markus , Csizi Katja-Sophia , Steiner Miguel 
Published: October 2023

Abstract

Nanoscopic systems exhibit diverse molecular substructures by which they facilitate spe- cific functions. Theoretical models of them, which aim at describing, understanding, and predicting these capabilities, are difficult to build. Viable quantum-classical hybrid mod- els come with specific challenges regarding atomistic structure construction and quantum region selection. Moreover, if their dynamics are mapped onto a state-to-state mecha- nism such as a chemical reaction network, its exhaustive exploration will be impossible due to the combinatorial explosion of the reaction space. Here, we introduce a quantum magnifying glass that allows one to interactively manipulate nanoscale structures at the quantum level. The quantum magnifying glass seamlessly combines autonomous model parametrization, ultra-fast quantum mechanical calculations, and automated reaction ex- ploration. It represents a unique approach to investigate complex reaction sequences in a physically consistent manner with unprecedented effortlessness in real time. We demon- strate these features for reactions in bio-macromolecules and metal-organic frameworks, diverse systems that highlight general applicability.
PPI-hotspotID: A Method for Detecting Protein-Protein Interaction Hot Spots from the Free Protein Structure
Authors: Chen Yao Chi , Sargsyan Karen , Wright Jon D , Chen Yu-Hsien , Huang Yi-Shuian , Lim Carmay 
Published: October 2023

Abstract

Experimental detection of residues critical for protein-protein interactions (PPI) is a time-consuming, costly, and labor-intensive process. Hence, high-throughput PPI-hot spot prediction methods have been developed, but they have been validated using relatively small datasets, which may compromise their predictive reliability. Here, we introduce PPI-hotspotID, a novel method for identifying PPI-hot spots using the free protein structure, and validated it on the largest collection of experimentally confirmed PPI-hot spots to date. We show that PPI-hotspotID outperformed FTMap and SPOTONE, the only available webservers for predicting PPI hotspots given free protein structures and sequences, respectively. It also outperformed AlphaFold-Multimer in detecting PPI-hot spots using predicted interfaces. When combined with the AlphaFold-Multimer-predicted interface residues, PPI-HotspotID, yielded better performance than either method alone. Furthermore, we experimentally verified the PPI-hot spots of eukaryotic elongation factor 2 predicted by PPI-hotspotID. Notably, PPI-hotspotID unveils PPI-hot spots that are not obvious from complex structures, which only reveal interface residues, thus overlooking PPI-hot spots in indirect contact with binding partners. Thus, PPI-hotspotID serves as a valuable tool for understanding the mechanisms of PPIs and facilitating the design of novel drugs targeting these interactions. A freely accessible web server and the source code for PPI-hotspotID are available at https://ppihotspotid.limlab.dnsalias.org/ and https://github.com/wrigjz/ppihotspotid/, respectively.
Systematic videolaryngoscopy use: remain aware of the risks
Authors: Delatre Florian , Dubois Guillaume , Zaatar Rody , Bartoli Pauline , Radji Michael 
Published: October 2023

Abstract
Background:Videolaryngoscopy is a life-saving technique recommended in difficult intubation algorithms in both the operating room and critical care settings. It has demonstrated numerous advantages in terms of increasing the success rate of first-attempt intubations.
However, it is not without risks, notably in terms of injuries to the ENT area. These injuries and their incidence are very rarely described in the large trials promoting the routine use of videolaryngoscopy. This case-control study aims to highlight this risk in order to discuss the promotion of routine videolaryngoscopy use.
Case report: A 52-year-old woman undergoes an inguinal hernia repair under coelioscopy. After two failed attempts of direct laryngoscopy, intubation using videolaryngoscopy is successfully performed on the first attempt. However, in the postoperative period, a lesion resembling a perforation of the anterior pillar of the right tonsillar fossa is discovered. The injury heals well after 10 days of monitoring.
Discussion:Injuries to the ENT area following videolaryngoscopy are not uncommon and are even more frequent than after direct laryngoscopy. This can be explained by a false sense of security induced by a perfect view of the glottis on the screen, a pursuit of this perfect view even when it is not suitable for intubation with a glide scope, and the use of a too rigid stylet.
Conclusion:It is advisable to exercise caution regarding the routine use of videolaryngoscopy until more substantial studies assessing this specific risk are conducted.
Revisiting Cytomegalovirus Seroprevalence in Singapore's Antenatal Populations: A Two-Decade Update
Authors: Partana Pamela , Wan Wei Yee , Chow Venessa Xin Yu , Chan Jerry Kok Yen , Tan Lay Kok , Tan Wei Ching , Lim Gek Hsiang , Yang Liying 
Published: October 2023

Abstract

Purpose Cytomegalovirus (CMV) is the most common congenital infection in pregnancy with potential long-term adverse effects on the fetus. There is limited data on CMV seroprevalence in pregnant women in Singapore, with last reported study dating back over two decades. We look at the latest CMV seroprevalence in antenatal population in Singapore. Methods Between January 2021 and August 2021, 385 pregnant women receiving antenatal care at Singapore General Hospital were randomly selected for CMV IgG test to be performed on their blood samples collected during the first trimester of their pregnancies. Positivity for CMV IgG represents past exposure prior to pregnancy. Results Overall CMV seroprevalence was 71.7% (276/385). The trend of CMV IgG positivity increased with age, 68.3% in those aged 20–29, 72.5% in the 30–39 age group, and 79.0% in women over 40. Conclusions There is a declining trend in CMV seroprevalence among pregnant women in Singapore, which indicates that a substantial portion of this population faces the risk of primary maternal CMV infection during pregnancy. Emerging research suggests that prenatal treatment with valacyclovir effectively reduces the likelihood of vertical transmission. Considering this evidence, it is imperative to reevaluate the recommendations for universal maternal CMV screening during pregnancy.
Parental practices that influence children’s development: how often are they implemented and by whom. Results from the NASCITA Birth Cohort study
Authors: Pandolfini Chiara , Clavenna Antonio , Campi Rita , Cartabia Massimo , Finazzi Stefano , Bonati Maurizio 
Published: October 2023

Abstract

Purpose
To assess how commonly 15 parental practices, known to have positive effects on child and adult health, are carried out by families in Italy, if they are related, and which characteristics are associated with implementation.
Methods
Children participating in the NASCITA Cohort, a prospective study in which family pediatricians in Italy collect data on children and their families, were included if they had sufficient data. Data on practice implementation, socio-demographic characteristics, and interrelatedness between practices were analyzed.
Results
In all, 3337 children were included. Their mothers had an average age at birth of 33 years (range 17–52), and medium-high levels of education (85.8% of mothers) and employment (71.9%). No smoking or alcohol in pregnancy, supine infant sleeping position, and tummy time were the most commonly implemented practices (by over 85% of mothers, each), while the least common was exclusive breastfeeding at 6 months (28.3%). Parental practices are related and several socio-demographic characteristics influence their implementation, with mother’s educational level and geographic area of residence influencing most of the practices (each influencing 12 of 15 practices). Low educational level (OR 0.34), being born abroad (OR 0.43), and residing in the South (OR 0.49), most reduce the probability of implementing numerous supportive practices (all three P < 0.001).
Conclusions
Socio-demographic factors contribute significantly to carrying out supportive practices. Future interventions should address the identified inequalities, prioritizing families most in need. Direct involvement of pediatricians is warranted given their favorable position for promoting positive behaviors.