bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of gut microbiota-derived metabolites and extracellular vesicles on neurodegenerative disease in a gut-brain axis chip
Authors: Kim Na Yeon , Lee Ho Yeon , Choi Yoon Young , Mo Sung Jun , Jeon Soomin , Ha Jang Ho , Park Soo Dong , Shim Jae-Jung , Lee Jaehwan , Chung Bong Geun 
Published: February 2024

AbstractA new perspective suggests that a dynamic bidirectional communication system, often referred to as the microbiome-gut-brain axis, exists among the gut, its microbiome, and the central nervous system (CNS). This system may influence brain health and various brain-related diseases, especially in the realms of neurodevelopmental and neurodegenerative conditions. However, the exact mechanism is not yet understood. Metabolites or extracellular vesicles derived from microbes in the gut have the capacity to traverse the intestinal epithelial barrier or blood–brain barrier, gaining access to the systemic circulation. This phenomenon can initiate the physiological responses that directly or indirectly impact the CNS and its function. However, reliable and controllable tools are required to demonstrate the causal effects of gut microbial-derived substances on neurogenesis and neurodegenerative diseases. The integration of microfluidics enhances scientific research by providing advanced in vitro engineering models. In this study, we investigated the impact of microbe-derived metabolites and exosomes on neurodevelopment and neurodegenerative disorders using human induced pluripotent stem cells (iPSCs)-derived neurons in a gut-brain axis chip. While strain-specific, our findings indicate that both microbial-derived metabolites and exosomes exert the significant effects on neural growth, maturation, and synaptic plasticity. Therefore, our results suggest that metabolites and exosomes derived from microbes hold promise as potential candidates and strategies for addressing neurodevelopmental and neurodegenerative disorders.
Toxoplasma gondii molecular detection and phylogenetic analysis in hemodialysis patients from Khuzestan, Southwest Iran
Authors: Yarahmadi Saba , Saki Jasem , Arjmand Reza , Shahbazian Heshmatolah 
Published: February 2024

Abstract
Background
The diagnosis and genetic characterization of Toxoplasma gondii (T. gondii) infection can make a significant influence to the prevention of the dangerous consequences of toxoplasmosis, particularly in immunocompromised people.

Objective
The aim of this investigation was to assess the frequency and genotyping of T. gondii in blood samples of patients with hemodialysis.

Materials and methods
In the current investigation, a total of 379 blood samples were taken from subjects with hemodialysis who were referred to teaching hospital of Ahvaz in the southwest of Iran. The samples were evaluated using the Nested PCR by targeting the B1 gene, and then, sequencing and phylogenetic tree were constructed.

Results
T. gondii DNA was found in 112 (29.55%) of the blood samples by Nested PCR. Amplicons from T. gondii revealed high identity with GenBank sequences. The phylogenetic analysis revealed that all sequences were closely related to Type I of T. gondii.

Conclusion
Because of the high incidence of toxoplasmosis with type I prevalent in hemodialysis patients, we recommend a systematic screening for toxoplasmosis to carry out for monitoring the possible dissemination of toxoplasmosis during hemodialysis.
Assessment of two repairing techniques for a novel lithium disilicate-based ceramic
Authors: Galal Rami Mohamed , Hamza Ghada Ezzat , Elhotieby Menatallah Mohamed , Aldemery Yosra Mohamed 
Published: February 2024

Abstract
Background
This research was done to evaluate the bond strength using two repair methods with lithium disilicate ceramic.

Methods
Cojet and clearfil repair methods were used to repair lithium disilicate ceramic. The ceramic was sliced into slices before sintering (30 samples). Vita Ambria (Vita, Zahnfabrik, Germany) ceramic was used, surface-treated with the 2 repair systems and then bonded to composite. Samples are stored, thermocycling was done, and then, shear loading was done until fracture. Bond strength values were collected and statistically analysed.

Results
The results showed that the cojet surface treatment showed significantly more bond strength (14.43 ± 1.46 Mpa) than the clearfil surface treatment showing (4.16 ± 1.33) as indicated by paired t-test p ≤ 0.05.

Conclusions
Bond strength between composite and the ceramic Vita Ambria is better using cojet surface treatment than using the clearfil system.
Clinical and Histopathological Risk Factors for Radioactive Iodine–Refractory Follicular and Oncocytic Thyroid Carcinoma
Authors: Stegenga Merel T , van Velsen Evert F S , Oudijk Lindsey , Verburg Frederik A , van Ginhoven Tessa M , Peeters Robin P , Medici Marco , Visser W Edward , van Kemenade Folkert J 
Published: February 2024

Abstract

Context
Risk factors for radioactive iodine (RAI)-refractory disease in follicular (FTC) and oncocytic thyroid carcinoma (OTC) are unknown.

Objective
The aim of this study is to identify clinical and histopathological risk factors for RAI-refractory disease in FTC and OTC patients, facilitated by an extensive histopathological revision.

Methods
All adult FTC and OTC patients treated at Erasmus MC (the Netherlands) between 2000 and 2016 were retrospectively included. The 2015 American Thyroid Association guidelines were used to define RAI-refractory disease. An extensive histopathological revision was performed applying the 2022 World Health Organization Classification using PALGA, the Dutch Pathology Databank. Logistic regression was used to identify risk factors for RAI-refractory disease, stratified by histological subtype.

Results
Ninety FTC and 52 OTC patients were included, of whom 14 FTC (15.6%) and 22 OTC (42.3%) patients developed RAI-refractory disease over a follow-up time of 8.5 years. RAI-refractory disease occurred in OTC after fewer cycles than in FTC (2.0 [interquartile range (IQR): 1.0-2.0] vs 2.5 [IQR: 2.0-3.75]), and it substantially decreased 10-year disease-specific survival, especially in OTC (46.4%; FTC 85.7%). In FTC, risk factors were higher age at diagnosis, pT3/pT4 stage, N1 stage, widely invasive tumors, and extrathyroidal extension. N1 stage and M1 stage were the strongest risk factors in OTC, rather than histopathological characteristics of the primary tumor.

Conclusion
To our knowledge, this is the first study that correlates clinical and histopathological risk factors with RAI-refractory disease in FTC and OTC, facilitated by a histopathological revision. In FTC, risk factors for RAI-refractory disease were foremost histopathological characteristics of the primary tumor, whereas in OTC presentation with lymph node and distant metastasis was associated with RAI-refractory disease. Our data can help clinical decision-making, particularly in patients at risk for RAI-refractory disease.
Pharmacological treatment of obesity: an overview of different groups of anti-obesity drugs available in Poland
Authors: Woch Barbara , Bereza Dawid , Sokołowska Katarzyna , Kulak Maria , Moreau Igor , Polańska Paulina , Lang Miriam 
Published: February 2024

Introduction and Objective: Obesity, as one of the diseases of civilization, has become a significant problem in our time. Although the first ways of treating it appeared many years ago, pharmacotherapy of obesity has been developing significantly in recent years.
The purpose of this article is to introduce the obesity drugs that have been registered for the treatment of obesity in Poland and to describe a new substance called tirzepatide, which, although it does not have such registration yet, is one of the most promising medications of the near future.

State of Knowledge: Obesity is an urgent and growing problem. In Poland, about 25% of adults are obese, and this number is increasing over the years.
In Europe, four drugs belonging to three different groups are registered for the treatment of obesity: orlistat, a combined drug that includes naltrexone hydrochloride and bupropion hydrochloride, liraglutide, and semaglutide. Although the last drug achieves the best results in studies and is the substance most favored by doctors and patients, there is still much research on new substances and formulations, which would bring even better results and health benefits. One of these substances is tirzepatide, a drug registered in 2022 for the treatment of type 2 diabetes.

Summary: Nowadays in Poland, there are four drugs registered for obesity. Two of them are representatives of the group of GLP-1 analogues.
Pharmacological treatment of obesity has good results and is a relatively safe process that should be implemented in conjunction with lifestyle changes. The fifth substance described in this article is tirzepatide, a drug that, although not registered for the treatment of obesity yet, is for now one of the most promising substances among the new drugs.
High-sensitivity dual U-shaped PCF-SPR refractive index sensor for the detection of gas and liquid analytes
Authors: Luo Xingdi , Liu Wei , Lv Jingwei , Yang Lin , Wang Jianxin , Chu Paul K. , Liu Chao 
Published: February 2024

A dual U-shaped photonic crystal fiber (PCF) biochemical sensor based on surface plasmon resonance (SPR) is designed for the simultaneous detection of gas and liquid analytes, and the properties are analyzed by the full vector finite element method (FEM). SPR is excited by placing gold nanowires on the inner surface of the U-shaped device. In this technique, the traditional metal deposition process can be replaced, subsequently reducing the difficulty and complexity of actual production and improving the phase matching between the basic mode and plasmonic modes. To improve the detection properties, the structural parameters of the sensor including the air hole diameter, spacing, gold nanowire diameter, and polishing depth are optimized, and to better evaluate and analyze the sensing properties, the wavelength and amplitude modulation inquiry method is adopted. The results show that the maximum wavelength sensitivity (WS), amplitude sensitivity (AS), minimum resolution (R), and optimal FOM are 35,000 nm/RIU, 438.08RIU−1, 2.86×10−6RIU, and 165.16RIU−1, respectively. In addition, the sensor can detect analyte RIs between 1.00 and 1.36 for gas and liquid analytes simultaneously. Owing to the simple structure, low cost, and ambient-condition monitoring, the sensor has large potential in a myriad of applications including sewage treatment, food safety, humoral regulation, environmental and biological monitoring, and medical diagnosis.
Downregulation of the CD151 protects the cardiac function by the crosstalk between the endothelial cells and cardiomyocytes via exosomes
Authors: Jiang Luying , Liu Jingbo , Yang Zhenjia , Wang Jianyu , Ke Wenkai , Zhang Kaiyue , Zhang Chunran , Zuo Houjuan 
Published: February 2024

Background
Heart failure (HF) is the last stage in the progression of various cardiovascular diseases. Although it is documented that CD151 contributes to regulate the myocardial infarction, the function of CD151 on HF and involved mechanisms are still unclear.

Method and results
In the present study, we found that the recombinant adeno-associated virus (rAAV)-mediated endothelial cell-specific knockdown of CD151-transfected mice improved transverse aortic constriction (TAC)-induced cardiac function, attenuated myocardial hypertrophy and fibrosis, and increased coronary perfusion, whereas overexpression of the CD151 protein aggravated cardiac dysfunction and showed the opposite effects. In vitro, the cardiomyocytes hypertrophy induced by PE were significantly improved, while the proliferation and migration of cardiac fibroblasts (CFs) were significantly reduced, when co-cultured with the CD151-silenced endothelial cells (ECs). To further explore the mechanisms, the exosomes from the CD151-silenced ECs were taken by cardiomyocyte (CMs) and CFs, verified the intercellular communication. And the protective effects of CD151-silenced ECs were inhibited when exosome inhibitor (GW4869) was added. Additionally, a quantitative proteomics method was used to identify potential proteins in CD151-silenced EC exosomes. We found that the suppression of CD151 could regulate the PPAR signaling pathway via exosomes.

Conclusion
Our observations suggest that the downregulation of CD151 is an important positive regulator of cardiac function of heart failure, which can regulate exosome-stored proteins to play a role in the cellular interaction on the CMs and CFs. Modulating the exosome levels of ECs by reducing CD151 expression may offer novel therapeutic strategies and targets for HF treatment.
Past and future freshwater availability scenarios and their impact on the Indian Sundarbans ecosystem and fisheries
Authors: Das Lalu , Bhowmick Sayani , Goswami Purba , Choudhury Ratul Roy , Akhter Javed 
Published: February 2024

Abstract
The Indian Sundarbans is considered one of the zones of highest vulnerability in the world in terms of climate change. About 4.43 million people living in the Indian Sundarbans face a lack of freshwater availability due to the erratic behaviour of monsoon rains, frequent cyclonic storms, intrusion of saline water, and other factors, all of which affect the fisheries and agriculture activities of this area. In this study, estimates of freshwater availability through past and predicted future rainfall and evapotranspiration change scenarios in the Sundarbans are presented. Due to the lack of high-quality in-situ data, various sources of gridded rainfall and evapotranspiration data were used. Between 1948 and 2010, half of the 19 administrative blocks showed a decreasing trend of monsoonal rainfall while the rest showed an increasing trend. Freshwater availability showed a decreasing trend during the monsoon season over different blocks of the Sundarbans, which is a matter of great concern for fisheries and agricultural activities. Statistical downscaling was used to generate future rainfall and evapotranspiration scenarios, using coarse resolution Global Climate Models from the Coupled Model Intercomparison Project phase five for a smaller area like the Sundarbans. Downscaled Global Climate Models project an increasing trend in future monsoon rainfall in both RCP 4.5 and RCP 8.5 emission scenarios. The increasing rainfall can trigger excessive run-off and flooding, which would in turn affect aquaculture infrastructure and damage lentic aquaculture productions across the Sundarbans. However, increased rainfall may expand the flood plain area and extend the feeding grounds of fish. Hence, the impact of rainfall change is quite unpredictable. Proper adaptation techniques may be required to harness the positive impacts while preventing negative effects.
An Overview of Studies on the Influence of Domestic Verbal Violence on Children Personality Development
Authors: Meng Qingliang 
Published: February 2024

An increasing number of adolescents are experiencing psychological problems in China, which has affected their personality development. An overview of studies on the influence of domestic verbal violence on children personality development was made, followed by a comparison between the status-quo of the existing studies of the U.S.A. and China. Results indicate that one cause for children personality development problems may lie in parents’ ignorance of the effect of verbal violence. The paper also reveals scarce studies have been done to explore the effect of domestic verbal violence on child personality development, thus calling for more attention from both researchers and parents.
Pay as You Throw Threshold Tariff: Evidence on the Incentive to Recycle
Authors: Rizzo Leonzio , Secomandi Riccardo 
Published: February 2024

Abstract
We study the impact of the introduction of a pay as you throw tariff in Ferrara which presented a low status-quo level of waste recycling. We find that it increased the waste recycling share by 40 % points and decreased the total waste per capita by 30 % points. Our dataset allows the split of the overall effect on waste recycling, finding that 63 % of recycling is due to organic material and 37 % to multimaterial (paper, glass, and plastic). This result suggests that packaging does not constitute the major waste recycling collection. Moreover, we find both an increase in waste recycling and a decrease in total waste, contrary to other case studies with a higher starting level of waste recycling. This leads to the important conclusion that pricing waste is effective in reducing pollution if the waste recycling level is sufficiently low.
Sonochemical synthesis of gold nanoparticles mediated by potato starch: Its performance in the treatment of esophageal cancer
Authors: Liu Mingwei , Xue Xiaolei , Karmakar Bikash , Eltantawy Waleed , El-kott Attalla F. , El. Nashar Emam M. , Abd-Ella Eman M. 
Published: February 2024

Abstract
Economically viable and eco-friendly potato starch (PS) was employed to synthesize Au NPs under ultrasound irradiation. PS phytochemicals have the function of a green reductant as well as an efficient stabilizer template to cap and synthesize gold nanoparticles. Transmission electron microscopy (TEM), UV-Vis spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) were applied to investigate the structure of the synthesized PS-Au NPs nanocomposite. FESEM results showed that the obtained Au NPs were spherical and ∼30 nm in diameter; their crystalline nature was detected by XRD and TEM data. PS-Au NP nanocomposite shows high antioxidant effects against DPPH. The colorimetric MTT investigation was followed in the determination of anti-esophageal cancer properties of the PS-Au NP nanocomposite against KYSE-30 and FLO-1 cell lines. The findings indicate that in 3 days, the cancer cell survival percentage in various dilations reduced as much as the PS-Au NP nanocomposite concentration increased. The best anti-cancer effect of the PS-Au NP nanocomposite was reported at 1,000 μg/mL dilation. Through MTT cytotoxicity analysis the half-maximal inhibitory concentration of PS-Au nanocomposite or IC50 values against the KYSE-30 and FLO-1 esophageal carcinoma cells were found as 125 and 176 μg/mL, respectively. The data indicated that these PS-Au NP nanocomposites inhibited esophageal cancer cells more strongly than normal cells.
Invariance properties of limiting point processes and applications to clusters of extremes
Authors: Janßen Anja , Segers Johan 
Published: February 2024

Abstract
Motivated by examples from extreme value theory, but without using the theory of regularly varying time series or any assumptions about the marginal distribution, we introduce the general notion of a cluster process as a limiting point process of returns of a certain event in a time series. We explore general invariance properties of cluster processes that are implied by stationarity of the underlying time series. Of particular interest in applications are the cluster size distributions, and we derive general properties and interconnections between the size of an inspected and a typical cluster. While the extremal index commonly used in extreme value theory is often interpreted as the inverse of a “mean cluster size”, we point out that this only holds true for the expected value of the typical cluster size, caused by an effect very similar to the inspection paradox in renewal theory.
Finite normal subgroups of strongly verbally closed groups
Authors: Denissov Filipp D. 
Published: February 2024

Abstract
In a recent paper by A. A. Klyachko, V. Y. Miroshnichenko, and A. Y. Olshanskii, it is proven that the center of any finite strongly verbally closed group is a direct factor.
In this paper, we extend this result to the case of finite normal subgroups of any strongly verbally closed group.
It follows that finitely generated nilpotent groups with nonabelian torsion subgroups are not strongly verbally closed.
Stochastic perturbations in the semi-Lagrangian advection algorithm of the SL-AV global atmosphere model
Authors: Alipova Kseniya A. , Mizyak Vasiliy G. , Tolstykh Mikhail A. , Goyman Gordey S. 
Published: February 2024

Abstract
An algorithm for stochastic perturbation of the semi-Lagrangian trajectories is implemented in the ensemble weather prediction system based on the global atmosphere model SL-AV20 with a horizontal resolution of approximately 20 km, 51 vertical levels, and Local Ensemble Transform Kalman Filter (LETKF). The combined use of methods for stochastic perturbation of trajectories and the parameters and tendencies of subgrid-scale processes parameterizations allows to generate ensembles with a larger spread compared to ensembles without stochastic perturbations of trajectories. An improvement in probabilistic estimates of the ensemble forecasts for various variables is shown. The comparison of two versions of ensemble prediction system is presented.
Simulating the propagation of boundary-layer disturbances by solving boundary-value and initial-value problems
Authors: Zasko Grigory V. , Boiko Andrey V. , Demyanko Kirill V. , Nechepurenko Yuri M. 
Published: February 2024

Abstract
The article deals with the downstream propagation of small–amplitude disturbances of viscous incompressible laminar boundary layers, using the linearized equations for disturbance amplitudes. Two different methods are proposed. The first one solves a two-dimensional boundary-value problem, using a buffer-domain technique to mimic the outflow boundary condition. The second one solves a streamwise initial-value problem, using a spectral parabolization at each integration step. Both methods show good performance in simulating the propagation of the Tollmien–Schlichting waves and the Görtler vortices and can be applied to compute the spatial optimal disturbances.
Synthesis, anti-HIV and cytotoxicity evaluation of chiral 2,5-disubstituted 1,3,4-thiadiazole derivatives bearing the sulfonamide scaffold
Authors: Israr Anum , Hameed Shahid , Al-Masoudi Najim A. 
Published: February 2024

Abstract
In the present study, chiral 2-(4-substiuted phenyl)amino-5-[1-(4-substituted benzenesulphonamido)alkyl]-1,3,4-thiazdiazoles 5a–x were synthesized from enantiopure l-amino acids in a multistep sequence. The starting acids were reacted with arylsulphonyl chlorides to produce N-arylsulfonyl amino acids 1a–h, followed by esterification to obtain the corresponding esters 2a–h. Treatment of esters with hydrazine hydrate afforded the corresponding hydrazides 3a–h. The coupling of hydrazides with aryl isothiocyanates followed by cyclization gave the target thiadiazoles 5a–x in good yields. The new synthesized compounds were assayed against HIV-1 and HIV-2 in MT-4 cells using MTT assay. Compounds 5s, 5v and 5w showed IC50 values of > 1.58, >1.98 and > 2.04 μm with SI > 1, respectively, indicating that these compounds were cytotoxic at concentrations values of 1.58, 1.98 and 2.04 μm, respectively.
Greedy Heirs: Luke 12,13–15.16–21 between Hereditary Disputes and πλεονεξία
Authors: Marcello Fabrizio 
Published: February 2024

Abstract
The brief account of an inheritance dispute between brothers in Luke 12,13–15 marks a turning point in the broader discourse of Luke 12,1–34 and also serves as an introduction to the subsequent parable of the rich fool (12,16–21). The purpose of this study is threefold. First, a careful analysis of the scene shows how Luke employs the legal institution of the arbitratum to shape the episode. Second, nine petitions from Ptolemaic and Roman Egypt concerning inheritance disputes between siblings are selected in order to help imagine a similar social context in which familial greed could often degenerate into physical violence or other forms of abuse. Finally, the often neglected consequences of the problem of inheritance are explored in relation to the following parable, which curiously centers on a question from God about the future destination of goods (12,20b).