bluuebunny/crossref_metadata_2025_split huggingface dataset:
Faktor-faktor yang Mempengaruhi Penggunaan Metode Kontrasepsi Jangka Panjang (MKJP) pada Pasangan Usia Subur di Indonesia: Literature Review
Authors: Mujahadatuljannah Mujahadatuljannah , Indriani Indriani , Rabiatunnisa Rabiatunnisa 
Published: February 2024

Pada negara-negara berkembang tingginya angka kematian ibu dan kehamilan yang tidak diinginkan masih menjadi masalah kesehatan masyarakat global. Hal ini terutama dipengaruhi oleh rendahnya penggunaan kontrasepsi pada masyarakat. Berdasarkan data di atas setiap tahun masih banyak peserta KB yang memilih metode kontrasepsi jangka pendek dibandingkan Metode Kontrasepsi Jangka Panjang (MKJP). Hal ini disebabkan oleh berbagai faktor seperti usia wanita, lama menikah, pendidikan, paritas (jumlah anak), budaya, agama, dan factor perbedaan jenis kelamin. Tujuan tinjauan ini adalah untuk mengidentifikasi faktor-faktor yang mempengaruhi penggunaan Metode Kontrasepsi Jangka Panjang (MKJP) pada pasangan usia subur (PUS) di Indonesia. Metode penelitian ini merupakan publikasi jurnal dengan ringkasan literatur, pencarian artikel menggunakan studi banding database komputerisasi (Google Scholar dan PubMed). Hasil: usia dewasa, pendidikan tinggi, wanita bekerja, jumlah anak yang lebih dari 2 orang, pengetahuan baik, sikap yang mendukung, adanya dukungan/partisipasi suami, pendapatan yang tinggi dan tempat tinggal di perkotaan mempengaruhi pasangan usia subur dalam memilih metode kontrasepsi jangka panjang (MKJP). Kesimpulan: Faktor-faktor yang mempengaruhi penggunaan metode kontrasepsi jangka panjang (MKJP) pada pasangan usia subur (PUS) di Indonesia adalah usia, pendidikan, pekerjaan, paritas, pengetahuan, sikap, partisipasi suami/dukungan suami, pendapatan/status ekonomi dan tempat tinggal.
Pengaruh Pemberian Sari Kurma (Phoenix Dactylifera) dan Ekstrak Belimbing Wuluh (Averhoa Blimbi L.) Terhadap Kadar Hemoglobin Darah (HB) Ibu Hamil di Wilayah Kerja Puskesmas Rasau Jaya
Authors: Dahliansyah Dahliansyah , Ginting Martinus , Hariyadi Didik 
Published: February 2024

Anemia merupakan masalah kesehatan serius yang memerlukan perhatian khusus karena dapat meningkatkan angka kesakitan dan kematian ibu serta dapat mempengaruhi pregnancy outcome. Penyebab utama anemia yaitu kekurangan zat besi yang disertai dengan zat gizi lainnya seperti asam folat, vitamin B12 dan vitamin A. Masalah anemia di indonesia tahun 2013 sebesar 37,1%, dan meningkat menjadi 48,9% tahun 2018. Upaya yang diberikan sudah banyak, salah satunya dengan suplementasi tablet besi (Fe) kepada ibu hamil sebanyak 90 tablet selama kehamila. Namun angka anemia masih tetap tinggi, sehingga perlu diberikan alternatif dalam pencegahannya, salah satunya dengan memberikan sari kurma dan ekstrak belimbing wuluh yang akan dibuat menjadi formula, diberikan kepada ibu hamil untuk dikonsumsi. Kurma (Phoenix Dactylifera), memliki kelebihan mengandung karbohidrat tinggi dapat membantu meningkatkan kadar hemoglobin. Sedangkan belimbing wuluh (Averhoa Blimbi L), yang dilengkapi dengan Vitamin C, kalsium, fosfor, zat besi, kalsium dan kalium dapat dikombinasikan dengan sari kurma, untuk memepercepat penyerapan zat besi. Tujuan penelitian mengetahui pengaruh pemberian Sari Kurma dan Ekstrak Belimbing Wuluh Terhadap Kadar Hemoglobin Darah (Hb) dan Kalium Ibu Hamil di wilayah Kerja Puskesmas Rasau Jaya. Jenis Penelitian ini adalah Quasi-Eksperimen dengan rancangan pretest-posttest with control group design. Populasi dalam penelitian adalah ibu hamil yang berada di wilayah Puskesmas rasau Jaya. Sedangkan sampel dalam penelitia ini adalah ibu hamil terpilih, dihitung menggunakan rumus Lameshow didapat sebanyak 30 sampel. Analisis statistik yang akan dilakukan meliputi analisis univariabel, bivariabel dengan paired t tes. Hasil penelitian didapat diperoleh peningkatan sebesar 0,39 dengan nilai p-value sebesar 0,000. Rata-rata nilai kadar hemoglobin darah pada sampel mengalami peningkatan sesudah diberikan sari kurma dan ekstrak belimbing wuluh. Kesimpulan. Ada pengaruh peningkatan kadar hemoglobin darah ibu hamil dengan pemberian sari kurma dan ekstrak belimbing wuluh.
Characterization and the Effect of Different Parameters on Photocatalytic Activity of Montmorillonite/TiO2 Nanocomposite under UVC Irradiation
Authors: Dao Tam Thi Bang , Ha Loan Thi Thu , Le Nhien Hon , Nguyen Do Trung , Nguyen Truong Huu , Ha-Thuc Chi-Nhan 
Published: February 2024

This study aimed to modify montmorillonite (MMT) with titanium dioxide (TiO2) by wet stirring method combined with ultrasonic to form MMT/TiO2 nanocomposite and used as a photocatalyst in the removal of organic dye rhodamine B (RhB). The characteristics of the synthesized samples were analyzed by methods such as energy-dispersive X-ray spectroscopy (EDX), scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and UV-Vis diffuse reflectance spectra (UV-Vis DRS). The degradation of RhB was carried out for 210 min under UVC irradiation, and the decolorization efficiency of RhB was evaluated by UV-vis spectroscopy. The results show that the TiO2 anatase nanoparticles are randomly distributed on the surface or the space between the MMT sheets to form a house-of-card structure. After 210 min of exposure under a UVC light source, the decolorization efficiency reached 91.5% for the solution with pH = 6.8, photocatalyst content 0.1 g/L, initial concentration RhB 10 mg/L, and UVC power 15 W. Liquid chromatography–mass spectrometry (LCMS) identified the degradation intermediates that MMT/TiO2 successfully cleaved the chromophore structure and formed more minor broken-ring by-products. The influence of operating parameters on RhB removal efficiency, including solution pH, photocatalyst content, initial dye concentration, inorganic, and organic scavengers, was studied. In addition, the kinetic modeling study shows that the RhB photodegradation reaction is consistent with the Langmuir-Hinshelwood first-order kinetic model.
Development and Validation of the Cell-based Functional Method for Neutralizing Anti-adalimumab Antibodies Detection in Human Serum
Authors: Nikiforova M. A. , Valouev I. A. , Petrov A. V. , Beketov E. E. , Shokhin I. E. 
Published: February 2024

Introduction. Adalimumab, a fully humanized monoclonal antibody, is a tumor necrosis factor (TNFα) inactivator that is used against a number of autoimmune diseases such as rheumatoid arthritis and other most common inflammatory arthropathies (ankylosing spondylitis, psoriatic arthritis). Despite the proven efficacy of adalimumab treatment, there is a risk of adverse events, tied up with the formation of anti-drug antibodies, including neutralizing antibodies. Currently, the evaluation and characterization of neutralizing antibodies has become an important part of clinical trials in the development of new drugs and biosimilars.Aim. The aim of this study is to develop and validate the cell-based functional method for neutralizing anti-adalimumab antibodies determination in human serum.Materials and methods. For determination of neutralizing anti-adalimumab antibodies, the cell line L-929 has been employed. L-929 is a cell line sensitive to the TNFα-mediated apoptosis; the neutralizing antibodies interact with adalimumab that leads to TNFα-mediated cytotoxicity. Cytotoxicity was measured using resazurin, an aromatic compound that is a redox indicator.Results and discussion. The developed method was validated for cut point, selectivity, sensitivity, precision, specificity and stability (short- and long-term). An important part of a method development for determining neutralizing antibodies is the selection of concentrations of TNFα (4 ng/ml) and adalimumab (250 ng/ml), as well as determining the minimum required dilution – this parameter is established as 1 : 20. Cut point was chosen as a «floating» cut point, and a correction factor (normalization factor) was determined equal to 0,86. The sensitivity of the developed method was estimated at 108,9 ng/ml of neutralizing anti-adalimumab antibodies.Conclusion. The obtained results can be applied for determining anti-adalimumab neutralizing antibodies in the assessment of the adalimumab immunogenicity, including clinical trials.
High prevalence of pre-treatment and acquired HIV-1 drug resistance mutations among non-citizens living with HIV in Botswana
Authors: Mokgethi Patrick T. , Choga Wonderful T. , Maruapula Dorcas , Moraka Natasha O. , Seatla Kaelo K. , Bareng Ontlametse T. , Ditshwanelo Doreen D. , Mulenga Graceful , Mohammed Terence , Kaumba Pearl M. , Chihungwa Moses , Marukutira Tafireyi , Moyo Sikhulile , Koofhethile Catherine K. , Dickinson Diana , Mpoloka Sununguko W. , Gaseitsiwe Simani 
Published: February 2024

BackgroundApproximately 30,000 non-citizens are living with HIV in Botswana, all of whom as of 2020 are eligible to receive free antiretroviral treatment (ART) within the country. We assessed the prevalence of HIV-1 mutational profiles [pre-treatment drug resistance (PDR) and acquired drug resistance (ADR)] among treatment-experienced (TE) and treatment-naïve (TN) non-citizens living with HIV in Botswana.MethodsA total of 152 non-citizens living with HIV were enrolled from a migrant HIV clinic at Independence Surgery, a private practice in Botswana from 2019–2021. Viral RNA isolated from plasma samples were genotyped for HIV drug resistance (HIVDR) using Sanger sequencing. Major known HIV drug resistance mutations (DRMs) in the pol region were determined using the Stanford HIV Drug Resistance Database. The proportions of HIV DRMs amongst TE and TN non-citizens were estimated with 95% confidence intervals (95% CI) and compared between the two groups.ResultsA total of 60/152 (39.5%) participants had a detectable viral load (VL) &gt;40 copies/mL and these were included in the subsequent analyses. The median age at enrollment was 43 years (Q1, Q3: 38–48). Among individuals with VL &gt; 40 copies/mL, 60% (36/60) were treatment-experienced with 53% (19/36) of them on Atripla. Genotyping had a 62% (37/60) success rate – 24 were TE, and 13 were TN. A total of 29 participants (78.4, 95% CI: 0.12–0.35) had major HIV DRMs, including at least one non-nucleoside reverse transcriptase inhibitor (NNRTI) associated DRM. In TE individuals, ADR to any antiretroviral drug was 83.3% (20/24), while for PDR was 69.2% (9/13). The most frequent DRMs were nucleoside reverse transcriptase inhibitors (NRTIs) M184V (62.1%, 18/29), NNRTIs V106M (41.4%, 12/29), and K103N (34.4%, 10/29). No integrase strand transfer inhibitor-associated DRMs were reported.ConclusionWe report high rates of PDR and ADR in ART-experienced and ART-naïve non-citizens, respectively, in Botswana. Given the uncertainty of time of HIV acquisition and treatment adherence levels in this population, routine HIV-1C VL monitoring coupled with HIVDR genotyping is crucial for long-term ART success.
Effects of fecal microbiota transplantation on metabolic health of DBA mice
Authors: Ye Wenxin , Fan Jinghui , Wu Wenzi , Chen Zhuo , Huang Qixin , Qian Lichun 
Published: February 2024

IntroductionNumerous studies have demonstrated that C57BL/6 mice exhibit superior growth rates and overall growth performance compared to DBA mice. To investigate whether this discrepancy in growth performance is linked to the composition of gut microorganisms, we conducted fecal microbiome transplantation (FMT) experiments.MethodsSpecifically, we transplanted fecal fluids from adult C57BL/6 mice, high-fat C57BL/6 mice, and Wistar rats into weaned DBA mice (0.2mL/d), and subsequently analyzed their gut contents and gene expression through 16S rRNA sequencing and transcriptome sequencing. During the test period, C57BL/6 mice and Wistar rats were provided with a normal diet, and high-fat C57BL/6 mice were provided with a high-fat diet.ResultsThe results of our study revealed that mice receiving FMT from all three donor groups exhibited significantly higher daily weight gain and serum triglyceride (TG) levels compared to mice of CK group. 16S rRNA sequensing unveiled substantial differences in the abundance and function of the gut microbiota between the FMT groups and the CK group. Transcriptome analysis revealed a total of 988 differential genes, consisting of 759 up-regulated genes and 187 down-regulated genes, between the three experimental groups and the CK group. Functional Gene Ontology (GO) annotation suggested that these genes were primarily linked to lipid metabolism, coagulation, and immunity. Pearson correlation analysis was performed on the differential genes and clusters, and it revealed significant correlations, mainly related to processes such as fatty acid metabolism, fat digestion and absorption, and cholesterol metabolism.DiscussionIn summary, FMT from dominant strains improved the growth performance of DBA mice, including body weight gain, institutional growth, and immune performance. This change may be due to the increase of probiotic content in the intestinal tract by FMT and subsequent alteration of intestinal gene expression. However, the effects of cross-species fecal transplantation on the intestinal flora and gene expression of recipient mice were not significant.
Case report: Concurrent low-volume subdural hematoma and ipsilateral ischemic stroke presenting as capsular warning syndrome: a complex case with anticoagulation dilemma and dual pathology
Authors: Strahnen Daniel , Stathi Angeliki , Beck Jürgen , Roelz Roland , Vasilikos Ioannis 
Published: February 2024

BackgroundThe simultaneous emergence of low-volume subdural hematoma and ipsilateral ischemic stroke in an atrial fibrillation patient who is under anticoagulation therapy is a rare and intricate clinical case. This report accentuates the diagnostic and treatment complexities associated with these consecutive neurological conditions.Case presentationAn 83 years-old male patient initially presented with acute dyspnea, raising the suspicion of pulmonary embolism. After exclusion of pulmonary embolism through CT angiography, the patient experienced a sudden onset of left-sided hemiparesis without prior history of head trauma but with chronic intake of apixaban due to atrial fibrillation. Subsequent cranial CT tomography revealed a small right parietal subdural hematoma. After reversal of the anticoagulation therapy, surgical evacuation of the subdural hematoma was successfully performed. However, in the postoperative period, the patient developed new neurological symptoms that could not be explained by the reduced size of the subdural hematoma on a follow-up CT scan. Cranial MRI revealed the coexistence of acute ischemic stroke in the right corona radiata. The recent surgical procedure precluded guideline-recommended stroke treatment.DiscussionThis case underscores the complexities of diagnosing and treating concomitant small volume subdural hematoma and ischemic stroke, especially if the latter occurs in the corona radiata resulting in fluctuating symptoms known as “capsular warning syndrome.” Reversal and secondary discontinuation of anticoagulant therapy for surgical intervention highlight the inherent risk of thrombotic events in anticoagulated patients. The development of tailored treatment strategies requires a multidisciplinary approach, and further research and guidelines are required in similar complex scenarios.ConclusionThe presence of both a small subdural hematoma and an ipsilateral ischemic stroke presenting as capsular warning syndrome in an anticoagulated patient highlights the intricacy of their care. This case calls for a comprehensive and collaborative strategy to address complicated clinical scenarios.
Potentially modifiable risk and protective factors affecting mental and emotional wellness in pregnancy
Authors: Wohrer Fiona , Ngo Helen , DiDomenico Jared , Ma Xingya , Roberts Melissa H. , Bakhireva Ludmila N. 
Published: February 2024

IntroductionImpaired mental and emotional wellness often co-occurs with prenatal substance use, and both affect infant socio-emotional, cognitive, language, motor, and adaptive behavioral outcomes. Guided by the modified biopsychosocial framework, this study examined the role of common substance exposures during pregnancy (i.e., alcohol and cannabis), socio-cultural factors (social support during pregnancy, adverse childhood experiences), and reproductive health factors on maternal mental health (MMH).MethodsData were obtained from a prospective cohort study–Ethanol, Neurodevelopment, Infant, and Child Health (ENRICH-2), and included 202 pregnant persons. Alcohol and cannabis exposures were assessed through repeated prospective interviews and a comprehensive battery of drug and ethanol biomarkers. MMH outcomes were evaluated during the third trimester through the Perceived Stress Scale, Edinburgh Depression Scale, Generalized Anxiety Disorders-7, and Post-traumatic Stress Disorder Checklist for Diagnostic and Statistical Manual of Mental Disorders. Univariate and multivariable linear regression models evaluated significant predictors of MMH.ResultsResults of multivariable analysis indicate that both maternal adverse childhood experiences and alcohol exposure, even at low-to-moderate levels, during pregnancy were associated with poorer scores for most MMH measures, while higher level of social support and Spanish as the primary language at home (as a proxy of enculturation) had protective effects (all p’s &lt; 0.05).ConclusionThese findings highlight the importance of assessing substance use, including periconceptional alcohol exposure, and mental health in pregnant persons as closely related risk factors which cannot be addressed in isolation. Our findings also emphasize a strong protective effect of socio-cultural factors on maternal mental and emotional wellbeing—a strong precursor to maternal-infant bonding and infant neurodevelopment.
Assessing gender differences in food preferences and physical activity: a population-based survey
Authors: Feraco Alessandra , Armani Andrea , Amoah Isaac , Guseva Elena , Camajani Elisabetta , Gorini Stefania , Strollo Rocky , Padua Elvira , Caprio Massimiliano , Lombardo Mauro 
Published: February 2024

IntroductionFood preferences are influenced by various factors, such as culture, age, and gender. The relationship between food tastes, meal preferences, and eating habits has been studied extensively in recent years; however, research on gender differences in these fields still needs to be addressed. The aim of this study was to investigate gender differences in food preferences and eating habits through self-administered questionnaires in a large Italian population sample.MethodsThe online survey included questions on food tastes, meal preferences, eating habits, and sport involvement.ResultsThe results of the study underline significant gender-specific dietary tendencies among the 2198 participants (1314 females and 884 males, average age 41.1 ± 12.7 yrs). The majority of subjects were in the annual income range between €20,000 and €40,000. Our analysis reveals significant gender differences in dietary preferences and eating habits. Men prefer red and processed meat, with significantly higher consumption rates than women. Women, on the other hand, show a greater inclination towards vegetables, whole grains, tofu, and high-cocoa-content dark chocolate, aligning with healthier food choices. The study also found differences in eating behaviors, including the frequency of meals, snacking habits, and hunger patterns: women tend to eat more frequently and report higher levels of hunger in the morning, while men tend to skip snacks. Furthermore, differences extend to eating contexts, such as the speed of eating, eating out, and eating alone, with men more likely to eat quickly and dine out. Episodes of uncontrolled eating without hunger also differ, with women reporting these behaviors more frequently than men. In addition, the analysis of sports preferences showed distinct patterns, with a lower percentage of women playing sports and those who do play sports preferring endurance and strength training, while men prefer strength training and endurance sports.DiscussionThese findings elucidate the complex interplay of biological, cultural, and gender-based factors in shaping dietary preferences and eating behaviors. In particular, our study reveals that gender dynamics significantly influence food choice and eating habits: women tend to choose healthier foods and eat regular meals, while men show preferences for specific tastes and meal-related behaviors. This analysis underscores the nuanced differences between male and female dietary patterns, influenced not only by inherent biological factors such as genetics and hormonal responses but also by societal norms and cultural contexts. Taken together, our results highlight the importance of integrating different perspectives, thus providing valuable insights into the development of public health strategies and tailored nutrition interventions aimed at chronic disease prevention.
“I loved before, but now I love even more.” Qualitative study of posttraumatic growth as a consequence of severe COVID-19 experience in Slovak adults
Authors: Tencerová Jana , Halama Peter , Uhrecký Branislav 
Published: February 2024

IntroductionThe results indicate that post-traumatic growth does indeed occur after overcoming the severe form of COVID-19. It suggests that this posttraumatic growth most often occurred through a reassessment of priorities and an appreciation of life itself and loved ones. COVID-19 disease has been one of the most discussed and researched topics for several years, as it dramatically affects everyone’s daily life.MethodsThe qualitative study presented here focuses on health psychology, especially post-traumatic growth after overcoming a severe form of the COVID-19 disease. We worked with adult people who had either been treated in the intensive care unit or had been hospitalized with severe pneumonia.ResultsThematic analysis was used to determine categories and subcategories. The study presented here contributes to knowledge about the COVID-19 experience by mapping a Slovakian sample of adult participants.DiscussionThe results obtained by Thematic analysis help us better understand how people experience the disease, especially those who have overcome a severe form of the disease and thus had a borderline experience when their lives were directly threatened, as well as their overall health.