bluuebunny/crossref_metadata_2025_split huggingface dataset:
BECLIN1 is essential for intestinal homeostasis
Authors: Lee Erinna , Tran Sharon , Juliani Juliani , Harris Tiffany , Evangelista Marco , Ratcliffe Julian , Ellis Sarah , Johnson Chad , Reehorst Camilla , Nightingale Rebecca , Luk Ian , Jenkins Laura , Ghilas Sonia , Buchert Michael , Lee James , De Cruz Peter , Duszyc Kinga , Yap Alpha , Gleeson Paul , Kile Benjamin , Mielke Lisa , Mariadason John , Fairlie Walter , Yakou Marina , Baloyan David , Inguanti Chantelle 
Published: November 2023

Abstract
BECLIN1 is a component of Class III phosphatidylinositol 3-kinase complexes that orchestrates autophagy initiation and endocytic trafficking. Here we show intestinal epithelium-specific BECLIN1 deletion in adult mice led to rapid fatal enteritis with compromised gut barrier integrity, highlighting its intrinsic critical role in gut maintenance. BECLIN1-deficient intestinal epithelial cells exhibited extensive apoptosis, impaired autophagy, and stressed endoplasmic reticulum and mitochondria. Remaining absorptive enterocytes and secretory cells displayed morphological abnormalities. Deletion of the autophagy regulator, ATG7, failed to elicit similar effects, suggesting novel autophagy-independent functions of BECLIN1 distinct from ATG7. Indeed, organoids derived from BECLIN1 KO mice showed E-cadherin mislocalisation providing a mechanism linking endocytic trafficking mediated by Beclin1 and loss of intestinal barrier integrity. Our findings establish an indispensable role of BECLIN1 in maintaining mammalian intestinal homeostasis and uncover its involvement in endocytic trafficking in this process. Hence, this study has significant implications for our understanding of intestinal pathophysiology.
A seven-Earth-radius helium-burning star inside a 20.5-min verification binary of GWs
Authors: Wang Xiaofeng , Lin Jie , Wu Chengyuan , Xiong Heran , Nemeth Peter , Han Zhanwen , Li Jiangdan , Elias-Rosa Nancy , Salmaso Irene , Filippenko Alexei , Brink Thomas , Yang Yi , Chen Xuefei , Yan Shengyu , Zhang Jujia , Guo Sufen , Cai Yongzhi , Mo Jun , Xi Gaobo , Liu Jialian , Guo Jincheng , Xia Qiqi , Xiang Danfeng , Li Gaici , Li Zhenwei , Zheng Weikang , Zhang Jicheng , Liu Qichun , Guo Fangzhou , Chen Liyang , Li Wenxiong 
Published: November 2023

Abstract
Binary evolution theory predicts that the second common envelope (CE) ejection can produce low-mass (0.32-0.36 M⊙) subdwarf~B (sdB) stars inside ultrashort-orbital-period binary systems as their helium cores are ignited under nondegenerate conditions. With the orbital decay driven by radiation of gravitational waves, the minimum orbital period that an sdB binary formed in this channel can reach before the Roche-lobe overflow is ~20 minutes. However, only four sdB binaries with orbital periods below an hour have been reported so far, while none of them has an orbital period approaching the above theoretical limit. This leads to an unclear role of the second CE ejection in forming ultracompact sdB binaries and a blocked link between detached helium-burning-star binaries and gravitational-wave sources, AM CVn stars. Here we report the discovery of a 20.5-minute-orbital-period ellipsoidal binary, TMTS J052610.43+593445.1, in which the visible helium-burning sdB star is being tidally deformed by an invisible carbon-oxygen white dwarf (WD) companion. This sdB star is inferred to have a mass of ~0.33 M⊙, approaching that of the helium-ignition limit. Owing to the low-mass sdB star, WD companion, and extremely short orbital period, this system provides the first clear case to justify the formation of sdB binaries through the nondegenerate channel of second CE ejection. In particular, the radius of this low-mass sdB star is only 0.066 R⊙, about seven Earth radii, representing the most compact nondegenerate star ever known. As the shortest-orbital-period single-degenerate detached binary discovered up to date, TMTS J052610.43+593445.1 provides a crucial bridge to map the binary evolution scheme from the second CE ejection to the formation of AM CVn stars having a helium-star donor, and will serve as a crucial verification binary of space-borne gravitational-wave detectors in the future.
Accelerating molecular docking using machine learning methods
Authors: Bande Abdulsalam Yazıd , Baday Sefer 
Published: November 2023

Abstract

Virtual screening (VS) is one of the well-established approaches in drug discovery which speeds up the search for a bioactive molecule and, reduces costs and efforts associated with experiments. VS helps to narrow down the search space of chemical space and allows selecting fewer and more probable candidate compounds for experimental testing. Docking calculations are one of the commonly used and highly appreciated structure-based drug discovery methods. Databases for chemical structures of small molecules have been growing rapidly. However, at the moment virtual screening of large libraries via docking is not very common. In this work, we aim to accelerate docking studies by predicting docking scores without explicitly performing docking calculations. We experimented with an attention based long short-term memory (LSTM) neural network for an efficient prediction of docking scores as well as other machine learning models such as XGBoost. By using docking scores of a small number of ligands we trained our models and predicted docking scores of a few million molecules. Specifically, we tested our approaches seven datasets that were produced in-house drug discovery studies. In one of the targets, by training only 7000 molecules we predicted docking scores for 3 million molecules with R2 (coefficient of determination) of 0.84. We designed the system with ease of use in mind. All the user needs to provide is a csv file containing smiles and their respective docking scores, the system then outputs a model that the user can use for the prediction of docking score for a new molecule.
Dutch Pharmacogenetics Working Group (DPWG) guideline for the gene-drug interaction of CYP2C9, HLA-A and HLA-B with anti-epileptic drugs
Authors: Nijenhuis Marga , Manson Lisanne , Soree Bianca , de Boer-Veger Nienke , Buunk Anne Marie , Houwink Elisa , Risselada Arne , Rongen Gerard , Schaik Ron van , Swen Jesse , Touw Daniel , Westrhenen Roos van , Deneer Vera , Guchelaar Henk-Jan 
Published: November 2023

Abstract
The Dutch Pharmacogenetics Working Group (DPWG) aims to facilitate PGx implementation by developing evidence-based pharmacogenetics guidelines to optimize pharmacotherapy. This guideline describes the gene-drug interaction of CYP2C9 and HLA-B with phenytoin, HLA-A and HLA-B with carbamazepine and HLA-B with oxcarbazepine and lamotrigine. A systematic review was performed and pharmacotherapeutic recommendations were developed. For CYP2C9 intermediate and poor metabolisers, the DPWG recommends lowering the daily dose of phenytoin and adjust based on effect and serum concentration after 7–10 days. For HLA-B*15:02 carriers, the risk of severe cutaneous adverse events associated with phenytoin, carbamazepine, oxcarbazepine and lamotrigine is strongly increased. For carbamazepine, this risk is also increased in HLA-B*15:11 and HLA-A*31:01 carriers. For HLA-B*15:02, HLA-B*15:11 and HLA-A*31:01 positive patients, the DPWG recommends choosing an alternative anti-epileptic drug. If not possible, it is recommended to advise the patient to report any rash while using carbamazepine, lamotrigine, oxcarbazepine or phenytoin immediately. Carbamazepine should not be used in an HLA-B*15:02 positive patient. DPWG considers CYP2C9 genotyping before the start of phenytoin “essential” for toxicity prevention. For patients with an ancestry in which the abovementioned HLA-alleles are prevalent, the DPWG considers HLA-B*15:02 genotyping before the start of carbamazepine, phenytoin, oxcarbazepine and lamotrigine “beneficial”, as well as genotyping for HLA-B*15:11 and HLA-A*31:01 before initiating carbamazepine.
Landslide Susceptibility Assessment using GIS-based multicriteria Decision Analysis (MCDA) along a part of National Highway-1, Kashmir- Himalayas, India
Authors: Beigh Iftikhar Hussain , Bukhari Kaiser 
Published: November 2023

Abstract

The current study aims at GIS-based multicriteria decision analysis (AHP) to generate a landslide-susceptible map from Baramulla to Uri Road segment along NH-1, Kashmir Himalaya, India. The landslide causative factors examined to generate our AHP matrix are slope gradient, elevation, slope aspect, curvature, distance to drainage, distance to roads, distance to lineaments, geology, land use land cover (LULC), and Rainfall. The study mapped and identified the active landslides along NH-1 through extensive field investigations and other secondary data sources. The landslide events were dominated by rockfall and debris slides. Based on their importance in landslide occurrences, the thematic layers were given relative relevance scores using Saaty's scale. Besides, the Analytic Hierarchy Process (AHP) was employed to normalize the relative weights and attributes of the various thematic layers. In addition, all thematic data layers were combined using a weighted linear approach to generate the landslide susceptibility (LS) map. Furthermore, the resultant LS map was classed into five categories viz., very high (24.18%), high (30.24%), medium (28.61%), low (15.28%), and very low (1.69%). The study reveals that 54.42% of the area falls under the high and very high susceptible zones. Likewise, 78.9% of overall model accuracy was computed from the Area under curve (AUC) method. Moreover, this study would aid infrastructural, geo-environmental, and landslide hazard planning in the studied region.
High uterosacral ligament suspension by transvaginal natural orifice transluminal endoscopic surgery or laparoendoscopic surgery for uterine prolapse: a protocol for a non-inferiority randomized controlled trial
Authors: Hou Tao , Chen Li , He Shan , Huang Lishan , Zhou Ping , Chen Yang , Liang Ye , Zhong Qijun , He Wen 
Published: November 2023

Abstract
Introduction:
Uterine prolapse is a prevalent health condition that significantly impairs the daily activities and quality of life of women. High uterosacral ligament suspension (HULS) through conventional laparoscopy (cLap) is a frequently employed surgical procedure for treating uterine prolapse. In recent years, gynecological surgeons have increasingly considered transvaginal natural orifice transluminal endoscopic surgery (vNOTES) due to its favorable outcomes, including enhanced cosmetic appearance and expedited postoperative recovery. However, there is a lack of studies comparing the techniques of vNOTES-HULS and cLap-HULS. Therefore, this trial aims to compare the efficacy and safety of vNOTES-HULS and cLap-HULS for the treatment of uterine prolapse at stage 2 or higher, providing valuable insights for surgical decision-making.
Methods
A two-arm, single-center, non-inferiority randomized controlled trial (RCT) will be conducted to evaluate whether the vNOTES-HULS procedure is non-inferior to the cLap-HULS procedure in the repair of uterine prolapse. The study aims to recruit a total of 116 patients diagnosed with stage 2 or above uterine prolapse from Meizhou People's Hospital. These patients will be randomly allocated to either the vNOTES-HULS or cLap-HULS intervention. The primary objective of the study is to determine the rate of successful uterine prolapse treatment during the 1-year follow-up evaluation. Secondary outcomes include perioperative parameters, complications, costs, postoperative recovery, and quality of life assessed at 1-month, 1-year, and 5-year intervals.
Discussion
This study aims to compare the effectiveness of vNOTES-HULS and cLap-HULS in the treatment of stage II or higher uterine prolapse based on the Pelvic Organ Prolapse Quantification classification.
Trial registration:
The study protocol received approval from the Research Ethics Committee of the Meizhou People’s Hospital (No. 2022-C-23) on October 3, 2022. It was registered into the Chinese Clinical Trials Registry on September 6, 2022 (no. ChiCTR2200063408). This study is currently in progress.
The Communication and Measurement Architecture of BDS-3 Global Operations and Services
Authors: LI Gang , GUO Shuren , GONG Wenbin , HAN Kai , GAO Weiguang , SHAO Fengwei , WANG Wenbin , TANG Chengpan , ZHANG Feng 
Published: November 2023

Abstract

As the BeiDou Navigation Satellite System (BDS) is developed form BDS-2regional services to BDS-3 global services, the regional-serving GEO and IGSOsatellites are transformed into global-serving MEO satellites. The observation arcof MEO satellites by regional ground stations is about 1/3. The accuracy of MEOorbit determination and time synchronization would become worse, which wouldmake the global positioning accuracy become unacceptable. It is very hard to pro-vide Radio Navigation Satellite System (RNSS) service and effectively managethe satellite constellation. Different from GPS, GLONASS and Galileo adopt-ing a worldwide ground-stations method, BDS-3 creatively established a globaloperations and services architecture with regional stations and Ka-band Inter-Satellite Links (ISLs). The architecture's contributions to BDS-3 include not onlycombined orbit determination and time synchronization, but autonomous navi-gation, global operations and services. The BDS-3 on-orbit operation results arepresented in the paper to verify the designed capabilities. Compared with BDS-2,the orbit determination accuracy is improved from 0.6m to 0.15m, and the time synchronization accuracy is improved from 6.67ns to 1.16ns. Under autonomousnavigation, the 30-day orbit maintenance accuracy reaches 16m and the timesynchronization accuracy reaches 3.74ns. The success rate of data transmissionreaches 99%, and the delay is better than 12.9s. In the future, laser ISLs will beused to enhance the system structure and further improve global operation andservice capabilities.
Hybrid abutment-Crowns with offset implant placement: Effect of titanium base height and machinable crown material on bacterial leakage
Authors: Alghannam Dina , Alzordok Walid , Attia Ahmed 
Published: November 2023

Abstract

Background
The purpose of the current study was to assess the effect of two different titanium base heights and three different machinable crown materials on bacterial leakage around hybrid-abutment crowns.
Methods
42 implant fixtures with typical external geometries hybrid-abutment crowns designed in CAD system were used. Samples were divided into six equal groups according to two variations, crown material, zirconia (Z), lithium disilicate (L), and hybrid ceramic (V) to accommodate two different heights of Ti-Base abutments either were short Ti-Bases (S) with 4 mm (n = 21) or long Ti-Bases (L) 7mm (n = 21). Each subgroup was equal 7 (n = 7), groups were (ZS), (ZL), (LS), (LL), (VS) and (VL). Adhesive resin cement with a universal primer were used according to manufacturer instructions. Artifactial aging was done through water storage for 30 days, chewing stimulating for samples (50,000-cycles, 49 N, 1.67 Hz) and thermal cycling with (5000 cycles at 5–55°C) were applied. Samples were incubated in a bacterial suspension then leakage recorded by counting through colony forming unite (CFU).
Results
The average bacterial leakage among different material used was (2.88 ± 1.01),short zirconia crown group (ZS) showed the least microbial leakage (1.86 ± 0.9) followed by (VL) (2.57 ± 0.7), (LL) (2.57 ± 1.2) then (ZL) that was (3.29 ± 0.7) and (LS) with (3.43 ± 0.7). the (VS) group observed with the highest bacterial leakage with (3.57 ± 0.5).
Conclusion
the CAD/CAM fabricated zirconia crowns can be used over other ceramic material in term of bacterial leakage around dental implant.
The prognostic impact of treatment centralization in patients with testicular germ cell tumors: Analysis of Hospital-Based Cancer Registry data in Japan
Authors: Suzuki Shuhei , Nagumo Yoshiyuki , Kandori Shuya , Kojo Kousuke , Nitta Satoshi , Chihara Ichiro , Shiga Masanobu , Ikeda Atsushi , Kawahara Takashi , Hoshi Akio , Negoro Hiromitsu , Bryan Mathis J , Okuyama Ayako , Higashi Takahiro , Nishiyama Hiroyuki 
Published: November 2023

Abstract
Background
To identify the prognostic impact of treatment centralization in patients with testicular germ cell tumors (TGCT).
Methods
We used a hospital-based cancer registry data in Japan to extract seminoma and non-seminoma cases that were diagnosed in 2013, histologically confirmed, and received the first course of treatment. To compare the 5-year overall survival (OS) rates of patients stratified by institutional care volume, we performed a Cox proportional hazards regression analysis using inverse probability of treatment weighting (IPTW) method to adjust patient backgrounds.
Results
A total of 1767 TGCT patients were identified. The 5-year OS rates for stage II and III TGCT patients treated at low-volume institutions (< 7 cases) were significantly worse than high-volume institutions ( > = 7 cases) (91.2% vs. 83.4%, p = 0.012). Histological stratification revealed that 5-year OS rates for stage II and III seminoma patients in the low-volume group were significantly worse than the high-volume group (93.5% vs. 84.5%, p = 0.041). Multivariate OS analysis using an IPTW-matched cohort showed that institutional care volume was an independent prognostic factor (hazard ratio 2.13 [95% confidence interval: 1.23–3.71], p = 0.0072).
Conclusion
Our results indicate that stage II and III TGCT patients experience lower survival rates at low-volume institutions and would benefit from treatment centralization.
Five year follow up after surgical treatment of type 2 diabetes with laparoscopic sleeve gastrectomy associated with a duodenal ileal interposition
Authors: Tinoco Augusto , NETTO MATHEUS SILVA , BENEDITO HENRIQUE , DEPAULA AUREO , KADRE LUCIANA EL 
Published: November 2023

Abstract

Introduction: Type 2 diabetes mellitus (T2D) is a chronic disease, primarily caused by a combination of defective insulin secretion and the inability of insulin-sensitive tissues to respond to insulin. Laparoscopic sleeve gastrectomy associated with duodenal ileal interposition (SGDII) has been shown to be a feasible treatment option for patients with T2D, as it provides improvement and control of glycemia, dyslipidemia and arterial hypertension. The aim of this study was to evaluate the mid and long-term results of SGDII for the treatment of diabetic patients, considering diabetic remission, weight loss and postoperative complications.
Materials and Methods: Retrospective study with 96 patients with T2D submitted to SGDII, between 2010 and 2016. The glycated hemoglobin (HbA1c) value < 6.5%, without the use of hypoglycemic agents, was considered as remission of T2D.
Results: The five year follow up demonstrated that 80% of patients had T2D remission at one year, 74.5% at three years and 61.8% at five years. The average duration of the disease was nine years and the mean glycated hemoglobin before surgery was 8.95%. Sixty-one patients (62.8%) were male, and 36 patients (37.2%) were female. The average age was 50.9 years. Median BMI was 33.43 kg/m2. The incidence of major postoperative complications in the first 30 days was 2.1%, with no mortality. Dyslipidemia control was sustained, after five years, in 62% of the patients.
Conclusion: SGDII resulted in good glycemic control at 5-years follow-up and represents a valid alternative for the treatment of T2D.
Two-year-span breast cancer screening uptake in Japan after the COVID-19 pandemic and its association with the COVID-19 vaccination
Authors: Abubakar Aminu Kende , Kaneda Yudai , Ozaki Akihiko , Saito Hiroaki , Murakami Michio , Hori Daisuke , Gonda Kenji , Tsubokura Masaharu , Tabuchi Takahiro 
Published: November 2023

Abstract

Purpose
There is a limited information on whether COVID-19 pandemic was associated with a decreased breast cancer screening uptake and if COVID-19 vaccination was associated with an increase of screening uptake. Our study explored a two-year span (2020 to 2022) uptake of breast cancer screening in Japan after the COVID-19 pandemic and assessed its association with the COVID-19 vaccination.
Methods
This is an analysis targeting the 6,100 women without cancer history who were aged 40 to 74 years at the 2021 survey of the Japan COVID-19 and Society Internet Survey (JACSIS), a web-based prospective cohort survey of 23,000 individuals, and also participated its 2022 survey. We examined the regular breast cancer screening uptake before the pandemic and two-year-span breast cancer screening uptake after the pandemic. We further employed multivariable Poisson regression model to seek any association between COVID-19 vaccination and screening uptake after the COVID-19 pandemic inception.
Result
Of 6,110, 38.2% regularly participated in the screening before the pandemic and 46.9% did so after the pandemic. Individuals unvaccinated due to health reasons (incidence rate ratio (IRR) = 0.47, 95% confidence interval: 0.29–0.77, p = 0.003) and for other reasons (IRR = 0.73, 95% confidence interval: 0.62–0.86, p < 0.001) were less likely to undergo screening compared to fully vaccinated individuals. There was no dose relationship between the vaccination and post-pandemic screening uptake.
Conclusion
There was no long-term decrease of the breast cancer screening uptake during the pandemic in Japan. The vaccination was linked to increased uptake, but the booster shot did not show a similar association with uptake.
Digital Foam Index Test for Air Entrainment Interactions
Authors: Baral Aniruddha , Roesler Jeffery R. 
Published: November 2023

Abstract
The digital foam index test (D-FIT) has been developed to quantify air-entraining agent (AEA) interaction with cement-fly ash-water mixtures and improve the repeatability of the traditional foam index test. The D-FIT acquires and analyzes videos of the evolving foam layer in a cementfly ash-water mixture as sequentially higher amounts of AEA are added. The proposed computer vision technique and deep-learning algorithm quantify the empty area on the surface and then calculates the percentage of the surface covered with foam. As part of the D-FIT, two new parameters have been defined. The AEA dosage required to produce a metastable foam is called the foam stability, whereas the incremental AEA dosage beyond the foam stability to achieve a foam layer covering the entire container surface is called the foam efficiency dosage. The summation of the foam stability and foam efficiency dosage is the traditional foam index value. Based on five ashes tested, the foam efficiency from vinsol resin-based AEA was higher than the sulfonate–based AEA, but the foam stability dosage was greater for the sulfonate–based AEA. Finally, the ratio of foam index to foam stability was not linked to the loss on ignition of ashes but more related to the interaction of the AEA type with the cement-fly ash mixture.