bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling antibacterial and antioxidant activities of zinc phosphate-based nanosheets synthesized by Aspergillus fumigatus and its application in sustainable decolorization of textile wastewater
Authors: El-Sharkawy Reyad M. , Abbas Mohamed H. H. 
Published: November 2023

Abstract
Background
The development of an environment-friendly nanomaterial with promising antimicrobial and antioxidant properties is highly desirable. The decolorization potentiality of toxic dyes using nanoparticles is a progressively serious worldwide issue.

Methods
The successful biosynthesis of zinc nanoparticles based on phosphates (ZnP-nps) was performed using the extracellular secretions of Aspergillus fumigatus. The antibacterial activity of the biosynthetic ZnP-nps was investigated against Gram-negative bacteria and Gram-positive bacteria using the agar diffusion assay method. The antioxidant property for the biosynthetic nanomaterial was evaluated by DPPH and H2O2 radical scavenging assay.

Results
Remarkable antibacterial and antiradical scavenging activities of ZnP-nps were observed in a dose-dependent manner. The minimum inhibitory concentration (MIC) for Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli was 25 µg/ml, however, the MIC for Bacillus subtilis was 12.5 µg/ml. The maximum adsorptive performance of nanomaterial was respectively achieved at initial dye concentration of 200 mg/L and 150 mg/L using methylene blue (MB) and methyl orange (MO), where sorbent dosages were 0.5 g for MB and 0.75 g for MB; pH was 8.0 for MB and 4.0 for MO; temperature was 30 °C; contact time was 120 min. The experimental data was better obeyed with Langmuir’s isotherm and pseudo-second-order kinetic model (R2 > 0.999). The maximum adsorption capacity (qmax) of MB and MO dyes on nanomaterial were 178.25 mg/g and 50.10 mg/g, respectively. The regenerated nanomaterial, respectively, persist > 90% and 60% for MB and MO after 6 successive cycles. The adsorption capacity of the prepared zinc phosphate nanosheets crystal toward MB and MO, in the present study, was comparable/superior with other previously engineered adsorbents.

Conclusions
Based on the above results, the biosynthesized ZnP-nanosheets are promising nanomaterial for their application in sustainable dye decolorization processes and they can be employed in controlling different pathogenic bacteria with a potential application as antiradical scavenging agent. Up to our knowledge, this is probably the first study conducted on the green synthesis of ZnP-nanosheets by filamentous fungus and its significant in sustainable dye decolorization.
Increased bacterial taxonomic and functional diversity is associated with impaired rotavirus vaccine immunogenicity in infants from India and Malawi
Authors: Cunningham-Oakes Edward , Bronowski Christina , Chinyama End , Jere Khuzwayo C. , Sindhu Kulandaipalayam Natarajan C. , Kang Gagandeep , Iturriza-Gómara Miren , Darby Alistair C. , Parker Edward P. K. 
Published: November 2023

AbstractThe immunogenicity and effectiveness of oral rotavirus vaccines (ORVs) against severe rotavirus-associated gastroenteritis are impaired in low- and middle-income countries (LMICs) where the burden of disease is highest. Determining risk factors for impaired ORV response may help identify strategies to enhance vaccine effectiveness. In this study, we use metagenomic sequencing to provide a high-resolution taxonomic analysis of stool samples collected at 6 weeks of age (coinciding with the first ORV dose) during a prospective study of ORV immunogenicity in India and Malawi. We then analyse the functional capacity of the developing microbiome in these cohorts. Microbiome composition differed significantly between countries, although functional capacity was more similar than taxonomic composition. Our results confirm previously reported findings that the developing microbiome is more diverse in taxonomic composition in ORV non-seroconverters compared with seroconverters, and we additionally demonstrate a similar pattern in functional capacity. Although taxonomic or functional feature abundances are poor predictors of ORV response, we show that skews in the direction of associations within these microbiome data can be used to identify consistent markers of ORV response across LMIC infant cohorts. We also highlight the systemic under-representation of reference genes from LMICs that limit functional annotation in our study (7% and 13% annotation at pathway and enzyme commission level, respectively). Overall, higher microbiome diversity in early life may act as marker for impaired ORV response in India and Malawi, whilst a holistic perspective of functional capacity may be hidden in the “dark matter” of the microbiome.
Differences between physician and patient preferences for cancer treatments: a systematic review
Authors: Zhang Mengqian , He Xiaoning , Wu Jing , Xie Feng 
Published: November 2023

Abstract
Background
Shared decision-making is useful to facilitate cancer treatment decisions. However, it is difficult to make treatment decisions when physician and patient preferences are different. This review aimed to summarize and compare the preferences for cancer treatments between physicians and patients.

Methods
A systematic literature search was conducted on PubMed, Embase, PsycINFO, CINAHL and Scopus. Studies elicited and compared preferences for cancer treatments between physicians and patients were included. Information about the study design and preference measuring attributes or questions were extracted. The available relative rank of every attribute in discrete choice experiment (DCE) studies and answers to preference measuring questions in non-DCE studies were summarized followed by a narrative synthesis to reflect the preference differences.

Results
Of 12,959 studies identified, 8290 were included in the title and abstract screening and 48 were included in the full text screening. Included 37 studies measured the preferences from six treatment-related aspects: health benefit, adverse effects, treatment process, cost, impact on quality of life, and provider qualification. The trade-off between health benefit and adverse effects was the main focus of the included studies. DCE studies showed patients gave a higher rank on health benefit and treatment process, while physicians gave a higher rank on adverse effects. Non-DCE studies suggested that patients were willing to take a higher risk of adverse effects or lower health benefit than physicians when accepting a treatment.

Conclusions
Physicians and patients had important preference differences for cancer treatment. More sufficient communication is needed in cancer treatment decision-making.
Understanding the management of pediatric spondylodiscitis based on existing literature; a systematic review
Authors: Lashkarbolouk Narges , Mazandarani Mahdi , Ilharreborde Brice , Nabian Mohammad Hossein 
Published: November 2023

Abstract
Background
Spondylodiscitis (SD), a rare disease in children, poses diagnostic challenges due to non-specific presenting symptoms, scarcity in incidence, and difficulty expressing pain in non-verbal children.

Method
A comprehensive search was conducted on three databases, including PubMed/Medline, Web of Science, and Scopus until March 2023. The inclusion criteria were studies that investigated the clinical characteristics, treatment, and complications of children’s spondylodiscitis. Full text of cross-sectional and cohort studies were added. The quality assessment of cohort studies was conducted using the Newcastle-Ottawa Quality Assessment Scale. The search, screening, and data extraction were performed by two researchers independently.

Result
Clinical manifestations of discitis in children are nonspecific, such as back pain, fever, reduced ability or inability to walk or sit, limping, and reduced range of movements. The mean delay in the time of diagnosis was 4.8 weeks. The most affected site of all the studies was the lumbar spine. 94% of studies reported increased inflammatory markers such as white blood cell count, C-reactive protein, and erythrocyte sedimentation rate. Less than 30% of patients had positive blood cultures and biopsy findings. The most common microbiological results (64%) were Staphylococcus Aureus and Kingella kingae. In radiographic evaluation, intervertebral disk narrowing, lumbar lordosis reduction, loss of disk height, and destruction of the vertebral body have been reported. In all studies, antibiotic therapy was initiated; in 52% immobilization was employed, and 29% of studies reported surgery was performed, and the follow-up period differed from 1.5 months to 156 months. 94% of studies reported complications such as vertebral body destruction, back pain, kyphosis, reduced range of movement, scoliosis, and neurological complications.

Conclusion
Spondylodiscitis is an uncommon, heterogeneous, multifactorial disease with resulting difficult and delayed diagnosis. Due to its morbidity, it is essential to investigate children with refusal to walk, gait disturbances, or back pain, particularly when associated with elevated inflammatory markers.
General and subspecialist pediatrician perspectives on barriers and strategies for referral: a latent profile analysis
Authors: Bohnhoff James C. , Guyon-Harris Katherine , Schweiberger Kelsey , Ray Kristin N. 
Published: November 2023

Abstract
Background
Children in need of pediatric subspecialty care may encounter multiple barriers, and multiple strategies have been suggested to improve access. The purpose of this study was to describe the perceptions of general and subspecialty pediatric physicians regarding barriers to subspecialty care and the value of strategies to improve subspecialty access.

Methods
We surveyed a national sample of 1680 general pediatricians and pediatric subspecialists in May and June 2020 regarding 11 barriers to subspecialty care and 9 strategies to improve access to subspecialty care, selected from recent literature. Using latent profile analysis, respondents were grouped according to the degree to which they believed each of the barriers impacted access to subspecialty care. Using chi-squared tests, we compared the profiles based on respondent characteristics and perspectives on strategies to improve access.

Results
The response rate was 17%. In 263 responses completed and eligible for inclusion, the barriers most frequently described as “major problems” were wait times (57%), lack of subspecialists (45%) and difficulty scheduling (41%). Respondents were classified into 4 profiles: “Broad concerns,” “Subspecialist availability concerns,” “Clinician communication concerns,” and “Few concerns.” These profiles varied significantly by respondent specialty (p < .001, with medical subspecialists overrepresented in the “Clinician communication” profile, psychiatrists in the “subspecialist availability” profile, and surgeons in the “few concerns” profile); and by respondents’ typical wait time for appointments (p < .001, with physicians with the longest wait times overrepresented in the “subspecialist availability” profile).

Conclusions
We found specific profiles in clinician views regarding barriers to subspecialty care which were associated with perspectives on strategies aimed at overcoming these barriers. These results suggest that health systems aiming to improve subspecialty access should first identify the barriers and preferences specific to local clinicians.
A multidisciplinary primary prevention intervention to increase adherence to the Mediterranean diet: a pilot study
Authors: De Marco Silvia , Marziali Eleonora , Nachira Lorenza , Arcaro Paola , Villani Leonardo , Galasso Valerio , Bruno Stefania , Laurenti Patrizia 
Published: November 2023

Abstract
Background
The role of the Mediterranean Diet (MD) in reducing cardiovascular (CV) risk is widely demonstrated and many studies have shown the effectiveness of educational interventions in primary prevention. This study aimed to evaluate the impact of a multidisciplinary educational intervention, that included nutritional, psychological and physical activity coaching, on adherence to MD and on CV risk.

Methods
In a Roman neighborhood, general practitioners enrolled 41 subjects to take part in the educational intervention from November 2018 (T0) to November 2019 (T1). Participants’ anthropometric measures, haematochemical parameters and CV risk score were assessed before and after the intervention. Furthermore, their adherence to MD was evaluated through the analysis of food frequency questionnaires using Medi-Lite.

Results
The study found a significant reduction of 2.5 points in individual CV risk score, and an increase of 2.5 point in adherence to the MD. The stratification by gender showed statistically significant decreases in weight of 1.16 kg, BMI of 0.47, LDL cholesterol of 14.00 mg/dL, and individual CV risk score of 1.16 points among female participants.

Conclusions
These results show that a multidisciplinary educational intervention model including the adoption of MD could be an effective strategy in Public Health for CV primary prevention and improvement of people’s lifestyles.
Cross-cultural adaptation and psychometric testing of the Yoruba lequesne algofunctional index of knee osteoarthritis among patients with knee osteoarthritis
Authors: Akinpelu Aderonke O. , Omosanya Oluwatosin J. , Odole Adesola C. , Adegoke Babatunde O.A. , Oyewole Olufemi O. 
Published: November 2023

Abstract
Background
The Lequesne Algofunctional Index of Knee Osteoarthritis (LAIKOA) is a widely used knee osteoarthritis (KOA) outcome measure and is recommended by many international authorities. It has been cross-culturally adapted to many languages, excluding indigenous Nigerian languages. The aim of this study was to cross-culturally adapt and validate the LAIKOA into Yoruba language.

Methods
This was a validation study. Yoruba LAIKOA was translated and culturally adapted from English version following Beaton’s guidelines (including cognitive debriefing). The Yoruba LAIKOA was psychometrically tested for test-retest reliability, standard error of measurements (SEM), smallest detectable change (SDC), internal consistency, and construct validity among 108 Yoruba-speaking patients with KOA recruited from selected hospitals in Ibadan, Nigeria. Participants completed the Yoruba and English versions of LAIKOA, and the Yoruba version of Ibadan Knee/Hip Osteoarthritis Outcome Measure (IKHOAM).

Results
The mean age of participants was 63.60 ± 11.77 years. Acceptable internal consistency was observed for the global index and function domain (α = 0.63–0.82) and good test-retest for items and domains (ICC = 0.81–0.995). Item-to-scale correlation was significant (r = 0.28–0.69). Its three domains demonstrated structural validity when subjected to confirmatory factor analysis (CFI = 0.99, TLI = 0.99, RMSEA = 0.02). Construct validity was supported by the correlation between Yoruba LAIKOA and IKHOAM (r = -0.39, p = 0.011). The overall scores and domain scores of the Yoruba and English versions of LAIKOA did not differ significantly. The Yoruba LAIKOA has no floor or ceiling effects.

Conclusion
The Yoruba LAIKOA is reliable and valid, and it is recommended for use in clinical settings in southwestern Nigeria and other Yoruba-speaking populations.
Kirschner wire versus external fixation in the treatment of proximal humeral fractures in older children and adolescents: a comparative study
Authors: Wang Yu , Wang Qian , Yao Wuyi , Zhao Jingxin , Zhao Xiaobin , He Man 
Published: November 2023

Abstract
Objective
The purpose of this study was to compare the therapeutic effects of Kirschner wire fixation and external fixation in the treatment of proximal humeral fractures in older children and adolescents.

Methods
A retrospective analysis was performed on the clinical data of older children and adolescents who underwent surgery at our institution for proximal humeral fractures between April 2014 and May 2022. One group (n = 28) underwent fracture reduction and Kirschner wire fixation, and the other group (n = 23) underwent external fixation. During the follow-up, the differences in shoulder joint function between the two groups were compared by analysing Quick Disabilities of the Arm, Shoulder, and Hand (Quick DASH) and Constant-Murley scores. Postoperative complications were also recorded.

Results
The operation time of the Kirschner wire group was shorter than that of the external fixation group (69.07 ± 11.34 min vs. 77.39 ± 15.74 min, P = 0.33). The time to remove the fixator in the external fixation group was shorter than that in the Kirschner wire group (6.74 ± 1.57 vs. 7.61 ± 1.22, P = 0.032). The Quick DASH score and Constant-Murley score of the patients in the external fixation group were significantly better than those in the Kirschner wire group at 3 months after surgery (5.63 ± 4.33 vs. 8.93 ± 6.40, P = 0.040; 93.78 ± 2.43 vs. 91.75 ± 2.15, P = 0.003). There was no significant difference in the Quick DASH score or Constant-Murley score between the patients in the external fixator group and those in the Kirschner wire group at 9 months after the operation (2.77 ± 3.14 vs. 3.17 ± 3.68, P = 0.683; 97.39 ± 1.80 vs. 96.57 ± 2.15, P = 0.152). The most common complication of the two groups was pin tract infection. The incidence rate of infection was higher in the external fixation group than that in the Kirschner wire group (9 vs. 4, P = 0.043).

Conclusion
Both Kirschner wire fixation and external fixation of N-H III and IV proximal humeral fractures in older children and adolescents produce good outcomes. External fixation is a preferred surgical treatment option for paediatric proximal humerus fractures because early mobilization of the affected limb can be realized.
Cementing techniques for total knee arthroplasty in Norwegian hospitals; a questionnaire-based study
Authors: Khan Michelle , Gjertsen Jan-Erik , Fenstad Anne Marie , Refsum Anders , Nguyen Uy , Hallan Geir , Høl Paul Johan , Furnes Ove 
Published: November 2023

Abstract
Background
Cementing technique in total knee arthroplasty (TKA) may influence implant survival. There is limited knowledge about the results with clinically used techniques.
The aim of this study was to investigate cementing techniques for TKA in Norwegian hospitals, to compare widely used techniques to recommendations from the literature, and to investigate variation within hospitals.

Methods
A questionnaire requesting information about cementing techniques were distributed to all Norwegian orthopedic surgeons performing TKAs regularly in 2020. Data was analyzed using descriptive statistical methods.

Results
We acquired 121 responses out of 257 surgeons. They were from 45 out of 56 hospitals, and at least half of the TKA surgeons from 20 hospitals, constituting 79 surgeons. All responders used pulsatile lavage. Cement application to both the tibial plateau and stem (full cementation) was practiced by 61%. Application of cement to both implant and bone was done by 70% of surgeons. Techniques to improve cement penetration were used by 86%. Only 35% of surgeons aimed to get a cement mantle thickness between 3–5 mm. Flexing the knee joint to remove excess cement was done by 82%. We found that in 55% of 20 hospitals the surgeons did not agree on the use of common guidelines in their ward.

Conclusions
The majority of the responders used recommended techniques from the literature when cementing TKA. At more than half of the eligible hospitals, surgeons disagreed about their hospitals’ use of common guidelines. Focusing on developing evidence-based guidelines would be beneficial for TKA-quality.
Comparative study on the clinical efficacy of small plate assisted anatomic plate and traditional double plate in the treatment of Rüedi and Allgöwer II - III pilon fracture
Authors: Ding Wenbin , Xu Jianing , Zhu Ya , Nian Shensheng , Lu Yifeng , Zheng Miaomiao , Zhu Xiang , Wang Zimin , Ji Fang , Tang Hao 
Published: November 2023

Abstract
Objective
The aim of this study was to investigate the clinical efficacy of small plate assisted anatomical plate and traditional double plate in the treatment of Rüedi and Allgöwer II - III pilon fracture.

Methods and materials
The data of 68 patients with pilon fracture admitted to Hospital from June 2017 to June 2020 were retrospectively analyzed. Study group and control group were divided according to different operation methods, with 34 cases in each group. There were 28 cases of Rüedi and Allgöwer II type and 40 cases of Rüedi and Allgöwer III type. Perioperative period data, Ankle joint function score, visual analog scale (VAS) scores and the incidence of incision complications were analyzed between these two groups.

Results
There were no significant differences in full load time, fracture healing time between these two groups (P > 0.05). The operation time, intraoperative blood loss, length of hospital stay, Ankle joint function score and postoperative incision complication rate in observation group were lower than those in control group (P < 0.05).

Conclusion
Small plate assisted anatomic plate is comparable to traditional double plate in the treatment of pilon fracture in terms of complete loading time, fracture healing time, but the former can shorten the operation time, reduce intraoperative blood loss and effectively reduce the incidence of postoperative complications.
Failure in Double-J stent inserting in laparoscopic pyeloplasty of ureteropelvic junction obstruction: the clinical features and outcomes
Authors: Wang Xinyu , Li Jiayi , Fan Songqiao , Li Zonghan , Yang Zhenzhen , Liu Pei , Song Hongcheng , Zhang Weiping 
Published: November 2023

Abstract
Background
Double-J (DJ) stent placement is an important procedure during laparoscopic pyeloplasty (LP). Failing to insert the DJ stent may indicate the patient was also complicated with uretero-vesical junction obstruction (UVJO), and surgeons have to change to another alternative drainage method. In the present study, we analyzed the risk factors of failure of DJ stent placement during the LP and reviewed the clinical outcomes of these challenging pyeloplasties.

Methods
We retrospectively analyzed the clinical data of patients with ureteropelvic junction obstruction (UPJO) who underwent LP in our department from January 2016 to September 2020. For patients who developed a difficult process of inserting the DJ stent, the externalized uretero-pyelostomy (EUP) stent was indwelled. Patients were finally divided into two groups: DJ group and EUP group. The primary outcomes were recurrent UPJO, postoperative uretero-vesical junction obstruction (UVJO) and complications.

Results
A total of 535 patients were included in the study, of which 37 patients (6.9%) failed to insert the DJ stent. Age was younger, and weight was lower (P < 0.05) in the EUP group. Within follow-up, recurrent UPJO occurred in ten (1.87%) patients, nine in the DJ group and one in the EUP group (P > 0.05). The incidence of postoperative UVJO in the EUP group was significantly higher than in the DJ group (10.8% vs. 0.2%, P < 0.01). 74 patients (13.8%) developed complications after surgery, 12 patients (32.4%) in the EUP group, significantly higher than that in the DJ group (32.4% vs. 12.4%, P < 0.01). Compared with the DJ group, the larger APD were observed in the EUP group at three months postoperatively (3.50 [3.02;4.58] vs. 2.20 [1.50;2.88], P < 0.05), but the difference vanished in further follow-up.

Conclusion
The failure of DJ stent placement tends to occur in patients with younger age, lower weight, and larger preoperative APD. Failure may not increase the recurrent UPJO rate, but may indicate a higher probability of postoperative UVJO and may develop more postoperative complications and slower recovery.