bluuebunny/crossref_metadata_2025_split huggingface dataset:
High prevalence of Prdm9-independent recombination hotspots in placental mammals
Authors: Joseph J. , Prentout D. , Laverré A. , Tricou T. , Duret L. 
Published: November 2023

AbstractIn many mammals, recombination events are concentrated into hotspots directed by a sequence specific DNA-binding protein named Prdm9. This protein facilitates chromosome pairing and its inactivation has been shown to induce fertility losses in mice and rats. Intriguingly,Prdm9has been lost several times in vertebrates, and notably among mammals, it has been pseudogenized in the ancestor of canids (dogs, wolves foxes). When this gene is inactive, either naturally in dogs, or through knock-out experiments in mice, recombination hotspots still exist, but they tend to occur in promoter-like features such as CpG islands. It has thus been proposed that one role ofPrdm9could be to direct recombination away from those Prdm9-independent hotspots. However, the ability of Prdm9 to direct recombination hotspots has been assessed only in a handful of species, and a clear picture of how much recombination occurs outside of Prdm9-directed hotspots in mammals is still lacking. In this study, we derived an estimator of past recombination activity based on signatures of GC-biased gene conversion in substitution patterns. We applied it to quantify recombination activity in Prdm9-independent hotspots in 52 species of boreoeutherian mammals. We observed a wide range of recombination rate at these loci: several species (such as mice, humans, some felids or cetaceans) show a deficit of recombination, while a majority of mammals display a clear peak of recombination. Our results demonstrate that Prdm9-directed and Prdm9-independent hotspots can co-exist in mammals, and that their co-existence seem to be the rule rather than an exception.
Unique Hmgn2 Orthologous Variant Modulates Shape Preference Behavior in Medaka Fish
Authors: Masaki Yume , Inoue Shuntaro , Nakagawa Shinichi , Yokoi Saori 
Published: November 2023

AbstractDiversification of protein sequences contributes to the variation of physiological traits. In this study, using medaka fish (Oryzias latipes) as a model, we identified a novel protein variant influencing shape preference behavior. Re-analysis of sequencing data revealed thatLOC101156433, previously annotated as a non-protein- coding gene, encodes a divergent Hmgn2 variant undetectable by standard homology searches. This unique Hmgn2 in medaka shows non-standard subnuclear localization, and mutants exhibited a reduction in certain regions of the telencephalon and a loss of shape preference. These results not only establish a direct association between amino acid sequence variation and the development of new molecular properties and behavioral adaptations but also highlight new clues toward understanding the visual shape perception system in fish.
Osteoblast-Induced Collagen Alignment in a 3Din vitroBone Model
Authors: Schaart Judith M. , Lindert Mariska Kea-te , Roverts Rona , Nijhuis Wouter H. , Sommerdijk Nico , Akiva Anat 
Published: November 2023

AbstractThe bone extracellular matrix consists of a highly organized collagen matrix that is mineralized by hydroxyapatite. Even though the structure and composition of bone have been studied extensively, the mechanisms underlying collagen matrix organization remain elusive. In this study, we developed a 3D cell culture system in which osteogenic cells deposit an oriented collagen matrix, that is subsequently mineralized. Using live fluorescence imaging combined with volume electron microscopy, we visualize the organization of the cells and collagen in the cell culture. We show that the osteogenic cells are organizing the collagen matrix during development. Based on the observation of tunnel-like structures surrounded by aligned collagen in the center of the culture, we propose that osteoblasts organize the deposited collagen during migration towards the periphery of the culture. Overall, we show that cell-matrix interactions are involved in collagen alignment during early-stage osteogenesis and that the matrix is organized by the osteoblasts in the absence of osteoclast activity.
Restaurant food waste among Chinese consumers in a group context: an extended value-attitude-behaviour (VAB) hierarchy with information publicity
Authors: Long Fei , Ooi Can-Seng , Gui Ting , Ngah Abdul Hafaz 
Published: November 2023

PurposeThe objective of this study is to examine Chinese consumers' behavioural intentions to reduce restaurant food wastage (i.e. intentions to order moderate meal size, and to pack leftovers) in a group context from both psychological and cultural perspectives.Design/methodology/approachThis paper used an online self-administered survey for data collection. The measurements were either adopted or adapted from prior studies on pro-environmental behaviours. Using data from 311 Chinese consumers, the authors analysed restaurant food wastage in China by utilising PLS-SEM.FindingsBy applying an extended value-attitude-behaviour (VAB) hierarchy with information publicity, it is found that materialism, frugality consciousness, information publicity and environmental concern are important factors in influencing individuals' behavioural intentions to reduce food waste in a group dining-out context.Originality/valueDrawing upon perspectives of Chinese consumers, this paper outlines key promoters and barriers to food waste mitigation, and provides meaningful insights to policymakers, NGOs, industry stakeholders and even consumers on how to effectively overcome the food waste challenge at the consumption stage in the context of Chinese culture.
A fuzzy multiobjective linear programming approach for green multiechelon distribution network design
Authors: Tai Pham Duc , Jinawat Krit , Buddhakulsomsiri Jirachai 
Published: November 2023

Purpose
Distribution network design involves a set of strategic decisions in supply chains because of their long-term impacts on the total logistics cost and environment. To incorporate a trade-off between financial and environmental aspects of these decisions, this paper aims to determine an optimal location, among candidate locations, of a new logistics center, its capacity, as well as optimal network flows for an existing distribution network, while concurrently minimizing the total logistics cost and gas emission. In addition, uncertainty in transportation and warehousing costs are considered.

Design/methodology/approach
The problem is formulated as a fuzzy multiobjective mathematical model. The effectiveness of this model is demonstrated using an industrial case study. The problem instance is a four-echelon distribution network with 22 products and a planning horizon of 20 periods. The model is solved by using the min–max and augmented ε-constraint methods with CPLEX as the solver. In addition to illustrating model’s applicability, the effect of choosing a new warehouse in the model is investigated through a scenario analysis.

Findings
For the applicability of the model, the results indicate that the augmented ε-constraint approach provides a set of Pareto solutions, which represents the ideal trade-off between the total logistics cost and gas emission. Through a case study problem instance, the augmented ε-constraint approach is recommended for similar network design problems. From a scenario analysis, when the operational cost of the new warehouse is within a specific fraction of the warehousing cost of third-party warehouses, the solution with the new warehouse outperforms that without the new warehouse with respective to financial and environmental objectives.

Originality/value
The proposed model is an effective decision support tool for management, who would like to assess the impact of network planning decisions on the performance of their supply chains with respect to both financial and environmental aspects under uncertainty.
Emerging adult gamers and their diet – a socio-ecological approach to improve health behaviour
Authors: Micallef David , Parker Lukas , Brennan Linda , Schivinski Bruno , Jackson Michaela 
Published: November 2023

Purpose
This paper aims to understand the opportunities and challenges to engage emerging adult gamers (aged 18–25) in adopting healthier diet behaviours through online games and related platforms such as esports and streaming. The study uses a socio-ecological approach to understand influences and suggests approaches to changing behaviours.

Design/methodology/approach
Purposive and convenience sampling were used to identify (n = 13) online gaming industry professionals and emerging adult (EA) gamers for interview. Qualitative thematic analysis of data using NVivo was undertaken.

Findings
Bi-directional influences were found that are potentially impacting EA diet behaviours. Food industry advertising and sponsorships were identified as dominant influences within the behavioural ecology, using microcelebrities and esports events to target EAs. The study identifies a need for social marketers to engage EA gamers in healthful behaviours through interventions across various levels of the behavioural ecology, including those upstream with industry and potential government regulation, to promote better health and balance food marketing. It also identifies future research avenues for engaging gamers in good health.

Originality/value
To the best of the authors’ knowledge, this is the first study to explore the impact of the gaming behavioural ecology on EA diet behaviour. It identifies new channels that social marketers can use to engage EAs, who are difficult to reach through more traditional marketing channels.
E-payment adoption and utilization among micro-entrepreneurs: a comparative analysis between Indonesia and Malaysia
Authors: Trianto Budi , Nik Azman Nik Hadiyan , Masrizal Masrizal 
Published: November 2023

Purpose
The development of financial technology (fintech), especially digital payments (e-payments), aims to increase the efficiency and effectiveness of economic transactions. This study aims to see the extent to which microentrepreneurs in Indonesia and Malaysia take advantage of the existence of e-payments in developing their business and the factors that influence the adoption of e-payments.

Design/methodology/approach
This study uses qualitative and quantitative approach. For quantitative approach, partial least squares structural equation modeling (PLS-SEM 4.0) was used to analyze the data. Using the nonprobability convenient sampling technique, this study collected 400 respondents from microenterprises in Indonesia and Malaysia in various regions.

Findings
Most of the microentrepreneurs in Indonesia and Malaysia have used fintech platforms, especially e-wallet and ATM debit. However, for quick response code-based fintech for business transactions, most microentrepreneurs have not taken advantage of the platform. Then the results of the digital payment adoption factor also differ for each country.

Research limitations/implications
This study is valuable for decision-makers and regulators. These results can be used to find a roadmap for regulators to build a digital economy, especially digital payments for microenterprises in both countries. In addition, these results can be used as a basis for making policies regarding digital payments.

Originality/value
To the best of the authors’ knowledge, this is the first study to compare e-payment adoption by microentrepreneurs in Indonesia and Malaysia. Indonesia and Malaysia are two countries in the Southeast Asia region that have great attention in fintech development. This study provides new insights about fintech, especially digital payments as a strategic approach in the digitalization era.
The relative valuation of cash flow and current accruals affected by their extremity
Authors: Mostafa Wael , Dixon Rob 
Published: November 2023

Purpose
Recent studies on the securities market’s differential pricing of earnings components have shown that cash flow from operations is more highly valued than total accruals and that moderate cash flow from operations has higher valuation than extreme total accruals. An interesting question that follows is whether these findings hold regarding the differential valuations of cash flow and current accruals. This study aims to extend prior research by addressing this issue in two ways. First, the authors examine the incremental information content of cash flow from operations beyond working capital from operations. Second, the authors assess the effect of extreme working capital from operations on the incremental information content of cash flow from operations. This study aims to extend prior research by addressing this issue in two ways.

Design/methodology/approach
This study adopts market-based accounting research to test its hypotheses and to achieve its objectives. Specifically, this study uses statistical associations between accounting data and stock returns to examine the incremental information content (value relevance) of cash flow and working capital from operations and the effect of extreme working capital from operations on the incremental information content of cash flow.

Findings
The results show that cash flow from operations is not more highly valued than current accruals (both being valued equivalently). However, moderate cash flow from operations has higher valuation than extreme current accruals (each is valued differently). Overall, these research findings indicate that cash flow becomes more important for valuation as accruals get “extreme”.

Practical implications
As accruals are unlikely to persist to be permanent across the years, these results can be interpreted as indicating that cash flow and accruals information are used jointly by investors, with one being more important than the other depending on the relative “extremeness” of each. Therefore, both are of value to the investor and both should be reported.

Originality/value
The paper contributes to the UK research on determining the preferred level of disaggregation of earnings components, i.e. operating cash flow, current accruals and non-current accruals. This would help investors to improve their investment and credit decisions.
Territorial disputes and affective polarization
Authors: BALCELLS LAIA , DANIELS LESLEY‐ANN , KUO ALEXANDER 
Published: November 2023

AbstractCan territorial disputes within countries be a basis for affective polarization? If so, how does it vary across territories? A burgeoning literature on affective polarization has largely focused on partisan divisions; we argue that contentious political issues such as those relating to territorial integrity can also be a basis for such affective polarization, where citizens feel concord with those sharing such policy preferences and animus for those who do not. We specify hypotheses about territorial‐policy‐based affective polarization and bring comparative survey evidence from three European regions with salient and contentious territorial claims: Scotland, Catalonia and Northern Ireland. While these three cases encompass different outcomes of territorial disputes, our results show strikingly similar levels of affective polarization.
A fast method for fitting integrated species distribution models
Authors: Dovers Elliot , Popovic Gordana C. , Warton David I. 
Published: November 2023

Abstract

Integrated distribution models (IDMs) predict where species might occur using data from multiple sources, a technique thought to be especially useful when data from any individual source are scarce. Recent advances allow us to fit such models with latent terms to account for dependence within and between data sources, but they are computationally challenging to fit.

We propose a fast new methodology for fitting integrated distribution models using presence/absence and presence‐only data, via a spatial random effects approach combined with automatic differentiation. We have written an R package (called scampr) for straightforward implementation of our approach.

We use simulation to demonstrate that our approach has comparable performance to INLA—a common framework for fitting IDMs—but with computation times up to an order of magnitude faster. We also use simulation to look at when IDMs can be expected to outperform models fitted to a single data source, and find that the amount of benefit gained from using an IDM is a function of the relative amount of additional information available from incorporating a second data source into the model. We apply our method to predict 29 plant species in NSW, Australia, and find particular benefit in predictive performance when data from a single source are scarce and when compared to models for presence‐only data.

Our faster methods of fitting IDMs make it feasible to more deeply explore the model space (e.g. comparing different ways to model latent terms), and in future work, to consider extensions to more complex models, for example the multi‐species setting.
Unmasking bipolarity in recurrent depressive disorder following herpes simplex virus triggered n‐methyl‐D‐aspartate encephalitis
Authors: Narasimhappa Karthik , Mamtani Harkishan , Jain Kshiteeja , Holla Vikram V. , Ganjekar Sundarnag , Manjunath Netravathi , Kulanthaivelu Karthik , Desai Geetha 
Published: November 2023

AbstractObjectiveHerpes simplex virus (HSV) infection triggered n‐methyl‐D‐aspartate (NMDA) encephalitis can lead to varied neuropsychiatric manifestations, including movement disorders and manic symptoms. HSV is known to affect the same brain regions as in secondary mania.MethodWe present a 35‐year‐old female diagnosed with recurrent depressive disorder (RDD) who developed NMDA encephalitis triggered by HSV infection.ResultHSV‐triggered NMDA encephalitis led to a manic switch in a woman with RDD on antidepressants, along with the new onset of dyskinetic movements.ConclusionA neurological insult predisposed our patient to the variable effects of antidepressant drugs.
Processing speed and sustained attention in bipolar disorder and major depressive disorder: A systematic review and meta‐analysis
Authors: Little Bethany , Anwyll Megan , Norsworthy Laura , Corbett Luke , Schultz‐Froggatt Mia , Gallagher Peter 
Published: November 2023

AbstractObjectivesCognitive impairment is a core feature of bipolar disorder (BD) and major depressive disorder (MDD). Deficits in processing speed (PS) and sustained attention (SA) may be particularly impaired and may underpin a broader profile of deficits, however current knowledge of the nature of these impairments is limited by heterogeneous results in the literature. Few reviews to date have attempted to disentangle sources of heterogeneity to assess the presence and magnitude of impairments in PS and SA in BD and MDD.MethodsOne hundred and three studies were reviewed to examine performance in tests of PS and SA in BD (n = 3452) and MDD (n = 5461) compared to healthy controls (n = 8016). Neuropsychological methodology used in the literature was summarised. Data were meta‐analysed to assess impairments in PS and SA for each neuropsychological test separately. Subgroup analysis was performed across mood states to investigate sources of heterogeneity.ResultsImpairments were found across most neuropsychological tests, with small to large effect sizes for BD (range: d = 0.19–0.96) and MDD (range: d = 0.29–0.86). Impairments were present in symptomatic states and euthymia in most cases. Some outcome measures were not impaired in euthymia. Heterogeneity was observed for most neuropsychological tests and remained after separating by mood state. There inadequate data to meta‐analyse some outcome measures, particularly for symptomatic groups.ConclusionImpairments in PS and SA in BD and MDD can be observed across most neuropsychological tests. Future research should further investigate the nature of these impairments across mood states, controlling for clinical confounds.
β‐elemene enhances cisplatin sensitivity of non‐small cell lung cancer cells via the miR‐17‐5p/STAT3 axis
Authors: Qian Tian , Wenxian Tong , Anbing He 
Published: November 2023

AbstractIn China, β‐elemene, a sesquiterpene compound derived from Curcuma wenyujin, is clinically used to treat many human malignancies, including non‐small cell lung cancer (NSCLC). Nonetheless, the role of β‐elemene in regulating cisplatin sensitivity of NSCLC cells and the related mechanisms are not clear. This study was conducted to investigate the role of β‐elemene in sensitizing NSCLC cells to cisplatin. In this work, cisplatin‐resistant NSCLC cell lines were constructed. CCK‐8, colony formation, and flow cytometry assays were executed to examine cell viability, growth, and apoptosis. MiR‐17‐5p and STAT3 expression levels in cells were detected by qRT‐PCR. Western blot was executed to determine the expression levels of STAT3 and apoptosis‐related proteins (Bax and Bcl‐2) in the cells. Dual‐luciferase reporter gene experiments were performed to verify the targeting relationship between miR‐17‐5p and STAT3. Herein, we report that, β‐elemene inhibits the viability, and induces the apoptosis of cisplatin‐resistant NSCLC cells. Additionally, β‐elemene induces the upregulation miR‐17‐5p and downregulation of STAT3. STAT3 is validated to be a target of miR‐17‐5p in NSCLC cells. Additionally, the role of β‐elemene to repress the viability of cisplatin‐resistant NSCLC cells is partially counteracted by miR‐17‐5p inhibitor or STAT3 overexpression. In summary, our study suggests that β‐elemene enhances cisplatin sensitivity of NSCLC cells by modulating miR‐17‐5p/STAT3 axis, and it may be a choice for the complementary treatment of NSCLC patients.
The effect of lower limb strengthening exercise on orthostatic blood pressure and the skeletal muscle pump in older people with orthostatic hypotension
Authors: Frith James , Robinson Lisa , Gibbon Jake Ryan , Allen John 
Published: November 2023

AbstractIntroductionActivation of muscles during standing is recommended to activate the skeletal muscle pump, increasing venous return and increasing blood pressure (BP) in people with orthostatic hypotension (OH).AimThe aim of this study is to determine if increasing the strength of the lower limb muscles can improve the effectiveness of the venous pump and postural BP in older people with OH.MethodsTen older people with OH underwent an 8‐week lower limb strengthening intervention. Repeated measurements of orthostatic BP, calf venous ejection fraction (EF) and muscle strength took place before, during and after intervention.ResultsThe intervention increased calf muscle strength by 21% (interquartile range: 18–28), p = 0.018, from a median baseline of 38 (34–45) kg. Participants had normal levels of venous EF 64% (51–75) at baseline, with little to no venous reflux. The median ejection volume at baseline was 44 (36–58) mL per calf. Despite increasing muscle strength, venous EF did not increase (percentage change −10% (−16 to 24), p = 0.8) and systolic BP drop did not improve (percentage change 0% (−17 to 16), p = 1.0). Similarly, visual analysis of individual case‐series trends revealed increasing muscle strength with no clinically meaningful change in EF or orthostatic BP.ConclusionsMuscle strengthening exercise does not increase the effectiveness of the skeletal muscle pump and is not an efficacious intervention for OH. As there is little to no venous pooling in the calf during standing in older people with OH, below knee compression is unlikely to be clinically effective.