bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy of GFR estimating equations based on creatinine, cystatin C or both in routine care
Authors: Fu Edouard L , Levey Andrew S , Coresh Josef , Grams Morgan E , Faucon Anne-Laure , Elinder Carl-Gustaf , Dekker Friedo W , Delanaye Pierre , Inker Lesley A , Carrero Juan-Jesus 
Published: October 2023

ABSTRACT

Background
New equations to estimate glomerular filtration rate based on creatinine (eGFRcr), cystatin C (eGFRcys) or both (eGFRcr-cys) have been developed by the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) and the European Kidney Function Consortium (EKFC). There is a need to evaluate the performance of these equations in diverse European settings to inform implementation decisions, especially among people with key comorbid conditions.

Methods
We performed a cross-sectional study including 6174 adults referred for single-point plasma clearance of iohexol in Stockholm, Sweden, with 9579 concurrent measurements of creatinine and cystatin C. We assessed the performance of the CKD-EPI 2009/2012/2021, EKFC 2021/2023, revised Lund-Malmö (RLM) 2011 and Caucasian, Asian, Pediatric and Adult (CAPA) 2014 equations against measured GFR (mGFR).

Results
Mean age was 56 years, median mGFR was 62 mL/min/1.73 m2 and 40% were female. Comorbid conditions were common: cardiovascular disease (30%), liver disease (28%), diabetes (26%) and cancer (26%). All eGFRcr-cys equations had small bias and P30 (the percentage of estimated values within 30% of mGFR) close to 90%, and performed better than eGFRcr or eGFRcys equations. Among eGFRcr equations, CKD-EPI 2009 and CKD-EPI 2021 showed larger bias and lower P30 than EKFC 2021 and RLM. There were no meaningful differences in performance across eGFRcys equations. Findings were consistent across comorbid conditions, and eGFRcr-cys equations showed good performance in patients with liver disease, cancer and heart failure.

Conclusions
In conclusion, eGFRcr-cys equations performed best, with minimal variation among equations in this Swedish cohort. The lower performance of CKD-EPI eGFRcr equations compared with EKFC and RLM may reflect differences in population characteristics and mGFR methods. Implementing eGFRcr equations will require a trade-off between accuracy and uniformity across regions.
Comparison meta-analysis of intraoperative MRI-guided needle biopsy versus conventional stereotactic needle biopsies
Authors: Dhawan Sanjay , Chen Clark C 
Published: October 2023

Abstract

Background
MRI-guided needle biopsy (INB) is an emerging alternative to conventional frame-based or frameless stereotactic needle biopsy (SNB). Studies of INB have been limited to select case series, and comparative studies between INB and SNB remain a missing gap in the literature. We performed a meta-analysis to compare INB and SNB literature in terms of diagnostic yield, surgical morbidity and mortality, tumor size, and procedural time.

Methods
We identified 36 separate cohorts in 26 studies of SNB (including both frameless and frame-based biopsies, 3374 patients) and 27 studies of INB (977 patients). Meta-regression and meta-analysis by proportions were performed.

Results
Relative to publications that studied SNB, publications studying INB more likely involved brain tumors located in the eloquent cerebrum (79.4% versus 62.6%, P = 0.004) or are smaller in maximal diameter (2.7 cm in INB group versus 3.6 cm in the SNB group, P = .032). Despite these differences, the pooled estimate of diagnostic yield for INB was higher than SNB (95.4% versus 92.3%, P = .026). The pooled estimate of surgical morbidity was higher in the SNB group (12.0%) relative to the INB group (6.1%) (P = .004). Mortality after the procedure was comparable between INB and SNB (1.7% versus 2.3%, P = .288). Procedural time was statistically comparable at 90.3 min (INB) and 103.7 min (SNB), respectively (P = .526).

Conclusions
Our meta-analysis indicates that, relative to SNB, INB is more often performed for the challenging, smaller-sized brain tumors located in the eloquent cerebrum. INB is associated with lower surgical morbidity and improved diagnostic yield.
NDRG1 promotes endothelial dysfunction and hypoxia-induced pulmonary hypertension by targeting TAF15
Authors: Li Chengwei , Lv Junzhu , Wumaier Gulinuer , Zhao Yu , Dong Liang , Zeng Yuzhen , Zhu Ning , Zhang Xiujuan , Wang Jing , Xia Jingwen , Li Shengqing 
Published: October 2023

Abstract

Background
Pulmonary hypertension (PH) represents a threatening pathophysiologic state that can be induced by chronic hypoxia and is characterized by extensive vascular remodeling. However, the mechanism underlying hypoxia-induced vascular remodeling is not fully elucidated.

Methods and Results
By using quantitative polymerase chain reactions, western blotting, and immunohistochemistry, we demonstrate that the expression of N-myc downstream regulated gene-1 (NDRG1) is markedly increased in hypoxia-stimulated endothelial cells in a time-dependent manner as well as in human and rat endothelium lesions. To determine the role of NDRG1 in endothelial dysfunction, we performed loss-of-function studies using NDRG1 short hairpin RNAs and NDRG1 over-expression plasmids. In vitro, silencing NDRG1 attenuated proliferation, migration, and tube formation of human pulmonary artery endothelial cells (HPAECs) under hypoxia, while NDRG1 over-expression promoted these behaviors of HPAECs. Mechanistically, NDRG1 can directly interact with TATA-box binding protein associated factor 15 (TAF15) and promote its nuclear localization. Knockdown of TAF15 abrogated the effect of NDRG1 on the proliferation, migration and tube formation capacity of HPAECs. Bioinformatics studies found that TAF15 was involved in regulating PI3K-Akt, p53, and hypoxia-inducible factor 1 (HIF-1) signaling pathways, which have been proved to be PH-related pathways. In addition, vascular remodeling and right ventricular hypertrophy induced by hypoxia were markedly alleviated in NDRG1 knock-down rats compared with their wild-type littermates.

Conclusions
Taken together, our results indicate that hypoxia-induced upregulation of NDRG1 contributes to endothelial dysfunction through targeting TAF15, which ultimately contributes to the development of hypoxia-induced PH.
Altered pain processing and sensitization in sickle cell disease: a scoping review of quantitative sensory testing findings
Authors: Kenney Martha O , Knisely Mitchell R , McGill Lakeya S , Campbell Claudia 
Published: October 2023

Abstract

Objectives
Over 50% of adults living with sickle cell disease (SCD) have chronic pain, but the underlying mechanisms of chronic pain in this population remain unclear. Quantitative sensory testing is an important measurement tool for understanding pain and sensory processing. This scoping review summarizes quantitative sensory testing methodologies used in sickle cell studies and the evidence for central sensitization in this population.

Methods
We conducted a systematic search of PubMed, Embase, and CINAHL to identify studies using quantitative sensory testing in individuals living with sickle cell disease. Search strategies were based on variations of the terms “sickle cell disease,” and “quantitative sensory testing.” Eligible studies were observational or experimental studies in human participants living with SCD that reported findings and detailed methodology for at least 1 quantitative sensory testing modality.

Results
Our search yielded a total of 274 records; 27 of which are included in this scoping review. Of the 27 studies, 17 were original studies (with combined total of 516 adult and 298 pediatric participants), and 10 were secondary or subgroup analyses of these prior studies. Significant variation existed in quantitative sensory testing methodologies across studies, including testing locations, type and intensity of stimuli, and interpretation of findings. Of the identified studies, 22% (2/9 studies) reported sensory abnormalities in mechanical sensitivity and thresholds, 22% (2/9 studies) reported abnormal pressure pain thresholds, 46% (6/13 studies) reported sensory abnormalities in thermal pain thresholds and tolerance (cold and warm), and 50% (2/4 studies) reported abnormalities in temporal summation.

Conclusion
Future studies should use standardized quantitative sensory testing protocols with consistent and operationalized definitions of sensitization to provide clear insight about pain processing and central sensitization in sickle cell disease.
Guiding Principles for the Practice of Integrative Physical Therapy
Authors: Justice Catherine , Sullivan Marlysa B , Van Demark Cheryl B , Davis Carol M , Erb Matt 
Published: October 2023

Abstract

Integrative health is an emerging specialty inside multiple disciplines within the medical community, yet the practice of integrative physical therapy remains undefined. This perspective paper suggests a set of guiding principles to support the role of physical therapy in integrative health. These guiding principles, including therapeutic partnership, whole person health, living systems, movement as an integrative experience, and salutogenesis, are described and explored in-depth as they relate to all aspects of patient care and clinician experience. These guiding principles are articulated within the context of social determinants of health and the interrelated roles that environment, trauma, stress, and lifestyle all play within an integrative physical therapy plan of care. Examples of current integrative physical therapy practices that embody these principles are described. The 5 guiding principles are designed to elicit interprofessional inquiry into how integrative health models can be applied to the art and science of physical therapy practice. The expansion of integrative health into the field of physical therapy has the potential to improve individual and population health, as integrative physical therapy can be used to address prevention, health promotion, primary care, and wellness while acknowledging the complex, dynamic, and interconnected nature of the human condition.

Impact
This perspective article presents 5 guiding principles to establish a framework to define and shape the growing application of an integrative health model to physical therapy practice. These integrative physical therapy guiding principles aim to improve the quality of whole-person, patient-centered care.
O-Linked N-Acetylglucosamine Transferase Ensures Survival of Mouse Fetal Liver Hematopoietic Progenitors Partly by Regulating Bcl-xL and Oxidative Phosphorylation
Authors: Soma Shunsuke , Murakami Koichi , Fukuchi Yumi , Kunimoto Hiroyoshi , Nakajima Hideaki 
Published: October 2023

Abstract
O-linked N-acetylglucosamine transferase (OGT) critically regulates wide variety of biological processes such as gene expression, metabolism, stress response, signaling and proteostasis. In adult hematopoiesis, OGT is crucial for differentiation of B and T cells and the maintenance of hematopoietic stem cells (HSCs). However, a role for OGT in fetal liver (FL) hematopoiesis remains unknown. To investigate a role for OGT in FL hematopoiesis, we conditionally disrupted OGT in hematopoietic cells in developing FLs. Hematopoietic specific disruption of OGT resulted in embryonic lethality in late stage of gestation due to severe anemia and growth retardation. OGT loss led to profound reduction of differentiating erythroid cells and erythroid progenitors in FLs due to massive apoptosis. In addition, clonogenic capacity of FL cells was severely impaired by OGT loss. Interestingly, expression of BCL-XL, a well-known inhibitor of apoptosis in FL cells, dramatically decreased, and the levels of reactive oxygen species (ROS) were increased in OGT-deficient FL cells. Overexpression of Bcl-xL and reduction of ROS significantly restored the colony formation of OGT-deficient FL cells. This study revealed a novel role for OGT during embryogenesis, which ensures survival of FL hematopoietic cells partly by regulating Bcl-xL and oxidative phosphorylation.
BLE beacons for sample position estimation in a life science automation laboratory
Authors: Wu Haiping , Junginger Steffen , Roddelkopf Thomas , Liu Hui , Thurow Kerstin 
Published: October 2023

AbstractEstimation of the sample position is essential for working process monitoring and management in the life science automation laboratory. Bluetooth low-energy (BLE) beacons have the advantages of low price, small size and low energy consumption, which make them a promising solution for sample position estimation in the automated laboratory. Several fingerprinting models have been proposed to achieve indoor localization with the received signal strength (RSS) data. However, most of the research depends on intensive beacon installation. Proximity estimation, which depends entirely on one beacon, is more suitable for sample position estimation in large automated laboratories. The complexity of the life science automation laboratory environment brings challenges to the traditional path loss model (PLM), which is a widely used radio wave propagation model-based proximity estimation method. In this paper, BLE sensing devices for sample position estimation are proposed. The BLE beacon-based proximity estimation is discussed in the framework of machine learning, in which the support vector regression (SVR) is utilized to model the nonlinear relationship between the RSS data and distance, and the Kalman filter is utilized to decrease the RSS data deviation. The experimental results over different environments indicate that the SVR outperforms the PLM significantly, and provides 1 m absolute errors for more than 95% of the testing samples. The Kalman filter brings benefits to stable distance predictions. Apart from proximity-based sample position estimation, the proposed framework turned out to be effective in position estimation between parallel workbenches and position estimation on an automated workstation.
Validation of a Clinical Calculator Predicting Freedom from Colon Cancer Recurrence after Surgery on the Basis of Molecular and Clinical Variables
Authors: Khan Asama , Thompson Hannah , Hsu Meier , Widmar Maria , Wei Iris H. , Pappou Emmanouil , Smith J. Joshua , Nash Garrett M. , Paty Philip B. , Garcia-Aguilar Julio , Shia Jinru , Gonen Mithat , Weiser Martin R. 
Published: October 2023

BACKGROUND:
The Memorial Sloan Kettering clinical calculator for estimating the likelihood of freedom from colon cancer recurrence on the basis of clinical and molecular variables was developed at a time when testing for microsatellite instability was performed selectively, based on patient age, family history, and histologic features. Microsatellite stability was assumed if no testing was done.

OBJECTIVE:
The objective of this study was to validate the calculator in a cohort of patients who had all been tested for microsatellite instability.

DESIGN:
Retrospective cohort analysis.

SETTING:
Comprehensive cancer center.

PATIENTS:
Consecutive colon cancer patients who underwent curative resection for stage I, II, or III colon cancer between 2017 and 2019.

MAIN OUTCOME MEASURES:
The calculator’s predictive accuracy was assessed using the concordance index and a calibration plot of predicted versus actual freedom from recurrence at 3 years after surgery. For the purpose of a secondary sensitivity analysis, the presence of a tumor deposit(s) (disease category N1c) was considered equivalent to one positive lymph node (category N1a).

RESULTS:
With median follow-up of 32 months among survivors, the concordance index for the 745 patients in the cohort was 0.748 (95% confidence interval, 0.693 to 0.801), and a plot of predicted versus observed recurrences approached the 45° diagonal, indicating good discrimination and calibration. In the secondary sensitivity analysis for tumor deposits, the concordance index was 0.755 (95% confidence interval, 0.700 to 0.806).

LIMITATIONS:
Retrospective design, single institution.

CONCLUSIONS:
These results, based on inclusion of actual rather than imputed microsatellite stability status and presence of tumor deposits, confirm the predictive accuracy and reliability of the calculator.
Self-management Behaviors and Associated Factors in Adult Cancer Survivors
Authors: Aung Mar Lar , Cheng Huilin 
Published: October 2023

Background
Despite numerous systematic reviews on self-management interventions for cancer survivors, little is known about survivors’ daily self-management practices and the influencing factors.

Objective
To identify self-management behaviors and related factors among cancer survivors.

Methods
Six databases were searched for primary quantitative, qualitative, and mixed-methods studies on self-management behaviors in cancer survivors that were published in English-language, peer-reviewed journals between 2012 and July 2022. The methodological quality of the included studies was evaluated using the Mixed Methods Appraisal Tool. The data extracted using a predetermined form were analyzed using qualitative content analysis.

Results
A total of 22 articles were included in the review; the majority were qualitative research, and half had moderate to high quality of evidence. Nine domains of self-management behaviors were identified, namely, physical activity/exercise, diet, emotional management, complementary and alternative medicine, symptom management, religiosity/spirituality, attending regular follow-ups, adjustment of other lifestyles, and medication management. Four categories of factors were associated with self-management behaviors in cancer survivors: (1) personal factors (demographic and psychological), (2) health status, (3) family factors, and (4) healthcare system. However, the relationships between self-management behaviors and these factors were mainly identified from qualitative studies or addressed in single studies.

Conclusions
Self-management behaviors in cancer survivors are multifaceted. There is an urgent need for quantitative exploration of factors associated with self-management in cancer survivors.

Implications for practice
This review provides nurses with a comprehensive basis for designing self-management support interventions for cancer survivors.
Patient-reported disease-specific concerns relating to sexuality in multiple myeloma
Authors: Henkelman Megan S. , Toivonen Kirsti I. , Tay Jason , Beattie Sara , Walker Lauren M. 
Published: October 2023

Abstract

Background:
Cancer has been increasingly recognized as having lasting effects on sexuality. Sexual function in breast and reproductive cancer survivors is better characterized, but the experiences of hematological cancer survivors remain understudied. Only one previous study acknowledges the existence of sexuality concerns among multiple myeloma (MM) survivors. This study explored MM patients' experiences of sexual expression and examined patient-provider communication about sexuality.

Methods:
Participants with MM (N = 65) were recruited internationally for an online survey about sexual experiences. Open-ended questions queried MM disease–specific experiences that may affect engagement in sexual activity, methods used to adapt to sexual changes, and communication with health care providers. Responses were analyzed using thematic content analysis.

Results:
Participants identified several barriers to sexual activity associated with MM, including concerns about bone health, infections, and vaginal bleeding and bruising. They also described adapting to sexual challenges by trying sexual medicines/aids, changing their mode of sexual expression, and managing treatment risks related to sexuality. Half of the participants reported sexuality discussions with providers, with a minority receiving sexual health-related recommendations.

Conclusions:
To date, the results of sexuality studies focus largely on hematological cancers as a group, without exploring specific disease experiences. Participants reported sexual difficulties common to other cancers but also some which may be unique to MM. Sexuality concerns were underaddressed by health care providers. Given that some sexuality concerns are related specifically to disease risk, discussions with oncology providers are of particular importance; therefore, specific suggestions for improving patient-provider communication and care are offered.
Clinical Significance of Pneumocephalus in Pediatric Mild Traumatic Brain Injury
Authors: Hanalioglu Damla , Elbir Cagri , Sahin Omer Selcuk , Ercandirli Aziz Kaan , Sahin Balkan , Turkoglu Mehmet Erhan , Kertmen Huseyin Hayri , Hanalioglu Sahin 
Published: October 2023

Objectives
Mild traumatic brain injury (mTBI) comprises most (70%–90%) of all pediatric head trauma cases seeking emergency care. Although most mTBI cases have normal initial head computed tomography scan, a considerable portion of the cases have intracranial imaging abnormalities on computed tomography scan. Whereas other intracranial pathological findings have been extensively studied, little is known about the clinical significance of pneumocephalus in pediatric mTBI.

Methods
We retrospectively identified pediatric mTBI patients with pneumocephalus using the institutional database of a large regional trauma referral center. Outcome measures were defined as clinically important TBI (ciTBI), hospitalization, intensive care unit (ICU) admission, and neurosurgical intervention. Comparisons were made between pneumocephalus and control (isolated linear fracture) groups as well as between isolated (only linear fracture and pneumocephalus) and nonisolated pneumocephalus (pneumocephalus and TBI) groups.

Results
Among 3524 pediatric mTBI cases, 43 cases had pneumocephalus (1.2%). Twenty-one cases (48.8%) had isolated pneumocephalus. The pneumocephalus group had higher rates of ciTBI, hospital admission, ICU admission, and neurosurgery when compared with the isolated linear fracture (control) group. The isolated pneumocephalus group had fewer ciTBI (21.1% vs 70%, P = 0.002), fewer hospitalization (23.8% vs 81.8%, P < 0.001), but similar ICU admission rates (4.8% vs 22.7%, P = 0.089) and length of hospital stay (4.0 ± 2.7 vs 3.6 ± 2.4 days, P = 0.798) in comparison to the nonisolated pneumocephalus group. None of the patients in the isolated group had neurosurgery whereas 2 patients in the nonisolated pneumocephalus group underwent surgery. Multivariable analysis revealed pneumocephalus as an independent predictor of ciTBI and hospital admission, but not ICU admission or neurosurgical intervention.

Conclusion
Pneumocephalus is associated with increased rates of hospitalization and ciTBI, but not ICU admission, unfavorable outcome, or neurosurgical intervention in pediatric mTBI. Although usually spontaneously resolving pathology, it may occasionally be linked with complications such as cerebrospinal fluid leakage, meningitis, and tension pneumocephalus. Therefore, careful evaluation, close observation, and early detection of complications may prevent adverse outcomes.