bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteotranscriptomic analyses of the midgut and Malpighian tubules after a sublethal concentration of Cry1Ab exposure on Spodoptera litura
Authors: Xu Ya‐Jing , Zhang Yu‐Ning , Xue‐Yang  , Hao Shao‐Peng , Wang Yan‐Jue , Yang Xiao‐Xue , Shen Ya‐Qin , Su Qing , Xiao Ying Dan , Liu Jian‐Qiu , Li Wan‐Shun , He Qi‐Hua , Chen Yue , Wang Li‐Ling , Guo Hui‐Zhen , Xia Qing‐You , Mita Kazuei 
Published: January 2024

AbstractBACKGROUNDCry1Ab has emerged as a bio‐insecticide to control Spodoptera litura (Lepidoptera: Noctuidae). However, the sublethal effects of Cry1Ab on the physiological changes and molecular level of S. litura have not been well documented. Our aims in this study were to assess the sublethal effect of Cry1Ab on S. litura, including midgut and Malpighian tubules as targets.RESULTSAfter sublethal Cry1Ab exposure, distinct histological alterations were mainly observed in the midgut. Furthermore, the results of comparative RNA sequencing and tandem mass tag‐based proteomics showed that, in the midgut, most differential expression genes (DEGs) were up‐regulated and significantly enriched in the serine protease activity pathway, and up‐regulated differential expression proteins (DEPs) were mainly associated with the oxidative phosphorylation pathway, whereas the down‐regulated involved in the ribosome pathways. In the Malpighian tubules, DEGs and DEPs were significantly enriched in the ribosome pathway. We proposed that ribosome may act as a universal target in energy metabolism with other pathways via the results of protein–protein interaction analysis. Further, by verification of the mRNA expression of some Cry protein receptor and detoxification genes after Cry1Ab treatment, it was suggested that the ribosomal proteins (RPs) possibly participate in influencing the Bt‐resistance of S. litura larvae under sublethal Cry1Ab exposure.CONCLUSIONUnder sublethal Cry1Ab exposure, the midgut of S. litura was damaged, and the proteotranscriptomic analysis elucidated that Cry1Ab disrupted the energy homeostasis of larvae. Furthermore, we emphasized the potential role of ribosomes in sublethal Cry1Ab exposure. © 2024 Society of Chemical Industry.
A Flow Cytometry Evaluation of Poly (1‐vinyl‐3‐alkyl imidazolium) Based Ionic Liquid's In Vitro Effect on Various Cellular Components of Blood
Authors: Panda Itishree , Bhola Rajesh Kumar , Samal Sangram Keshari , Pradhan Sanghamitra 
Published: January 2024

AbstractThis study aims to gain insight into the effect of the synthesized poly‐ionic liquids (PILs) (1‐vinyl‐3‐alkyl imidazolium bromide) (alkyl=hexyl (A), octyl (B), and decyl (C)) on various cellular components of blood by flow cytometry. PILs may have some adverse effect on blood depending on the nature and the dose administered. So, it is critical to understand the potential influences of foreign polymeric entities on blood before they are used clinically. The complete blood counts (CBCs) are done using an automated analyzer based on the flow cytometry principle. The leukocyte viability with different PIL samples is performed by flow cytometry using 7‐AAD. The auto‐hemolysis is performed by incubating the PILs with defibrinated blood. The CBC is mostly the same when incubated with various PILs. However, RBCs show an increase in mean corpuscular volume (MCV), indicating cellular swelling. The viability with phosphate saline buffer (PBS), dimethyl sulphoxide (DMSO), and PIL (A, B, and C) is 99.94 %, 99.76 %, 99.71 %, 98.87 %, and 99.42 %, respectively. The effect on leukocyte size & internal structures are noted. The auto‐hemolysis tests of RBC by PILs are very similar to control auto‐hemolysis.
Platelet‐Derived Growth Factor Nanocapsules with Tunable Controlled Release for Chronic Wound Healing
Authors: Tang Lin , Cai Susu , Lu Xing , Wu Dingqi , Zhang Yahan , Li Xiaoming , Qin Xiaoyan , Guo Jimin , Zhang Xiaopeng , Liu Chaoyong 
Published: January 2024

AbstractChronic wounds have emerged as an increasingly critical clinical challenge over the past few decades, due to their increasing incidence and socioeconomic burdens. Platelet‐derived growth factor (PDGF) plays a pivotal role in regulating processes such as fibroblast migration, proliferation, and vascular formation during the wound healing process. The delivery of PDGF offers great potential for expediting the healing of chronic wounds. However, the clinical effectiveness of PDGF in chronic wound healing is significantly hampered by its inability to maintain a stable concentration at the wound site over an extended period. In this study, a controlled PDGF delivery system based on nanocapsules is proposed. In this system, PDGF is encapsulated within a degradable polymer shell. The release rate of PDGF from these nanocapsules can be precisely adjusted by controlling the ratios of two crosslinkers with different degradation rates within the shells. As demonstrated in a diabetic wound model, improved therapeutic outcomes with PDGF nanocapsules (nPDGF) treatment are observed. This research introduces a novel PDGF delivery platform that holds promise for enhancing the effectiveness of chronic wound healing.
Investigation of reinforcement curtailment in tall lightly reinforced structural concrete walls
Authors: Deng Tianhua , Henry Richard S. 
Published: January 2024

SummaryRecent studies have investigated the impact of minimum longitudinal reinforcement limits on the ductility of the plastic hinge region of reinforced concrete (RC) walls, resulting in revised minimum reinforcement limits in design standards. The objective of this research was to investigate the influence of the longitudinal reinforcement distribution or termination rules for the additional reinforcement at the end regions of the wall up the wall height on the response of taller lightly RC walls. A model utilizing a displacement‐based fiber element was developed and validated against tested walls with a range of longitudinal reinforcement contents. Pushover analyses were then conducted on a 20‐story wall prototype to investigate the sensitivity of key design parameters, including axial load pattern, curtailment heights of the longitudinal reinforcement in the plastic hinge region, reinforcement content at wall base, reinforcement strain‐hardening ratio, and the length over which the reinforcement curtailment occurred. The results indicate that the current minimum reinforcement provisions in ACI 318–19 are insufficient to prevent reinforcement yielding outside the plastic hinge region at the wall base.
Hybrid electrochemical/membrane couplings processes for enhancing seawater pretreatment and desalination
Authors: Aouni Anissa , Tounakti Rim , Ahmed Badiaa Ait , Hafiane Amor 
Published: January 2024

AbstractThis research focuses on boosting seawater pretreatment and desalination through electrocoagulation (EC)/ultrafiltration (UF) and electrocoagulation (EC)/nanofiltration (NF) processes. We first optimized the key parameters of the EC process using aluminum (Al) and iron (Fe) electrodes. Experimental results show EC process is efficient under optimal conditions. Second, membrane filtration using UF (ES10B), NF(UTC60) and NF(200) as post‐processing steps to the EC process were experimented with. EC(Al)/NF(UTC60) combination resulted in the highest removal rate of organic matter (COD 98%, TOC 95%, fluorescence [humic and fulvic acids] 68%), optical density (OD600nm 75%, turbidity 70%, conductivity 64%). In terms of major ions removal, up to 55% was achieved as NF decreases conductivity, salinity, and hardness. EC(Al)/NF(UTC60) seawater permeate demonstrated the best results in terms of lowest flux decline (J/Jo = 0.9) and fouling, which was realized by resistance in series and recovery factor rate (%). Additionally, NF(UTC60) fouling reversibility led to a longer lifetime and higher recovery factor (93%).Practitioner Points
Pretreatment by hybrid processes was experimented with to enhance the saline water treatment.
Organic matter (COD 98%, TOC 95%, fluorescence [humic and fulvic acids] 68%) and turbidity were successfully removed.
Salinity and hardness (conductivity 64%) were highly reduced by NF.
Flux decline, retention rate, and membrane fouling were studied.
Ferroptosis regulation through Nrf2 and implications for neurodegenerative diseases
Authors: Xiang Yao , Song Xiaohua , Long Dingxin 
Published: January 2024

AbstractThis article provides an overview of the background knowledge of ferroptosis in the nervous system, as well as the key role of nuclear factor E2-related factor 2 (Nrf2) in regulating ferroptosis. The article takes Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS) as the starting point to explore the close association between Nrf2 and ferroptosis, which is of clear and significant importance for understanding the mechanism of neurodegenerative diseases (NDs) based on oxidative stress (OS). Accumulating evidence links ferroptosis to the pathogenesis of NDs. As the disease progresses, damage to the antioxidant system, excessive OS, and altered Nrf2 expression levels, especially the inhibition of ferroptosis by lipid peroxidation inhibitors and adaptive enhancement of Nrf2 signaling, demonstrate the potential clinical significance of Nrf2 in detecting and identifying ferroptosis, as well as targeted therapy for neuronal loss and mitochondrial dysfunction. These findings provide new insights and possibilities for the treatment and prevention of NDs.
High molecular/low acetylated chitosans reduce adhesion of Campylobacter jejuni to host cells by blocking JlpA
Authors: Kreling Vanessa , Falcone Franco H. , Herrmann Fabian , Kemper Leon , Amiteye Daniel , Cord-Landwehr Stefan , Kehrenberg Corinna , Moerschbacher Bruno M. , Hensel Andreas 
Published: January 2024

Abstract
Infections caused by Campylobacter spp. are a major cause of severe enteritis worldwide. Multifactorial prevention strategies are necessary to reduce the prevalence of Campylobacter. In particular, antiadhesive strategies with specific inhibitors of early host–pathogen interaction are promising approaches to reduce the bacterial load. An in vitro flow cytometric adhesion assay was established to study the influence of carbohydrates on the adhesion of C. jejuni to Caco-2 cells. Chitosans with a high degree of polymerization and low degree of acetylation were identified as potent antiadhesive compounds, exerting significant reduction of C. jejuni adhesion to Caco-2 cells at non-toxic concentrations. Antiadhesive and also anti-invasive effects were verified by confocal laser scanning microscopy. For target identification, C. jejuni adhesins FlpA and JlpA were expressed in Escherichia coli ArcticExpress, and the influence of chitosan on binding to fibronectin and HSP90α, respectively, was investigated. While no effects on FlpA binding were found, a strong inhibition of JlpA-HSP90α binding was observed. To simulate real-life conditions, chicken meat was inoculated with C. jejuni, treated with antiadhesive chitosan, and the bacterial load was quantified. A strong reduction of C. jejuni load was observed. Atomic force microscopy revealed morphological changes of C. jejuni after 2 h of chitosan treatment, indicating disturbance of the cell wall and sacculi formation by electrostatic interaction of positively charged chitosan with the negatively charged cell surface. In conclusion, our data indicate promising antiadhesive and anti-invasive potential of high molecular weight, strongly de-acetylated chitosans for reducing C. jejuni load in livestock and food production.

Key points
• Antiadhesive effects of chitosan with high DP/low DA against C. jejuni to host cells
• Specific targeting of JlpA/Hsp90α interaction by chitosan
• Meat treatment with chitosan reduces C. jejuni load
No evidence that recruitment pheromone modulates olfactory, visual, or spatial learning in the ant Lasius niger
Authors: Koch Alexandra , Kabas Melanie , Czaczkes Tomer J. 
Published: January 2024

Abstract
Pheromones are perhaps the most common form of intraspecific communication in the animal kingdom and used in various contexts. Their modulatory potential on cognitive processes has been demonstrated in both vertebrates and invertebrates. Particularly interesting in this regard are social insects, due to their extensive use of pheromones to organise collective behaviour. Recruitment pheromones might be expected to encourage learning, but could also hinder learning due to a blocking effect, whereby the pheromone already partially predicts the reward, hindering further cues being associated with the reward. Here, we use free-running learning assays using realistic pheromone strength to test for a modulation effect on learning in the black garden ant Lasius niger. We found no evidence that learning in three modalities (olfactory, visual, and spatial) is affected by the presence of a realistic pheromone trail. Interestingly, this is in contrast to findings in honeybees. The fact that associative learning does not seem to be influenced by recruitment pheromone in L. niger and reportedly the Argentine ant, while it is in honeybees, the possibly best-studied social insect species, is noteworthy. We speculate that a species-specific importance of social information use could drive modulatory effects of pheromones on a wide range of cognitive processes.

Significance statement
Pheromones have been shown to modulate associative learning in a variety of animals. Among social insects, attractive pheromone has been found to enhance associative olfactory learning in honeybees but not in ants. In ants, recruitment pheromone predicts a food source; therefore, it might hinder learning of a new cue for a food reward. We use a free-running learning assay to test for an effect of trail pheromone on associative learning in three different modalities—olfactory, spatial, visual—in Lasius niger, but find no evidence of any effect. Our learning assay demonstrated fast olfactory learning, moderate spatial learning, and no visual learning after only one training visit. Based on our findings, and findings in two other ant species, we speculate that the ecological foraging conditions of mass-recruiting ants, i.e. following a trail, have not favoured a modulation potential of recruitment pheromone opposed to attractive pheromone in honeybees.
Histone FRET reports the spatial heterogeneity in nanoscale chromatin architecture that is imparted by the epigenetic landscape at the level of single foci in an intact cell nucleus
Authors: Liang Zhen , Solano Ashleigh , Lou Jieqiong , Hinde Elizabeth 
Published: January 2024

AbstractGenome sequencing has identified hundreds of histone post-translational modifications (PTMs) that define an open or compact chromatin nanostructure at the level of nucleosome proximity, and therefore serve as activators or repressors of gene expression. Direct observation of this epigenetic mode of transcriptional regulation in an intact single nucleus, is however, a complex task. This is because despite the development of fluorescent probes that enable observation of specific histone PTMs and chromatin density, the changes in nucleosome proximity regulating gene expression occur on a spatial scale well below the diffraction limit of optical microscopy. In recent work, to address this research gap, we demonstrated that the phasor approach to fluorescence lifetime imaging microscopy (FLIM) of Förster resonance energy transfer (FRET) between fluorescently labelled histones core to the nucleosome, is a readout of chromatin nanostructure that can be multiplexed with immunofluorescence (IF) against specific histone PTMs. Here from application of this methodology to gold standard gene activators (H3K4Me3 and H3K9Ac) versus repressors (e.g., H3K9Me3 and H3K27Me), we find that while on average these histone marks do impart an open versus compact chromatin nanostructure, at the level of single chromatin foci, there is significant spatial heterogeneity. Collectively this study illustrates the importance of studying the epigenetic landscape as a function of space within intact nuclear architecture and opens the door for the study of chromatin foci sub-populations defined by combinations of histone marks, as is seen in the context of bivalent chromatin.
Impact of Reducing Fluoroscopy Pulse Rate on Adult Modified Barium Swallow Studies
Authors: Bonilha Heather Shaw , Reedy Erin L. , Wilmskoetter Janina , Nietert Paul J. , Martin-Harris Bonnie 
Published: January 2024

AbstractModified Barium Swallow Studies (MBSS) are a critical part of the evaluation, treatment planning, and outcome assessment for persons with swallowing disorders. Since MBSSs use ionizing radiation with associated cancer risks, many clinicians have reduced radiation exposure by reducing the fluoroscopic pulse rate. However, by reducing pulse rate, we also decrease the temporal resolution of MBSSs which has been shown in pilot studies to significantly reduce diagnostic accuracy. Two hundred MBSSs from patients routinely undergoing MBSS as standard of care conducted at 30 pulses per second (pps) using the Modified Barium Swallow Study Impairment Profile (MBSImP™) standardized administration protocol were selected. A stratified sampling method ensured that a full range of swallowing impairments (etiology, type, and severity) was represented. Recordings were down sampled from 30 pps to 15, 7.5, and 4 pps. MBSSs were rated using the MBSImP components and Penetration–Aspiration Scale (PAS) score for each swallow. Percent agreement was calculated across raters for MBSImP and PAS scores by bolus type and volume. The Least-Squares Method was used for hypothesis testing. Statistically significant and clinically meaningful changes in scores of swallowing physiology and penetration/aspiration occurred when reducing pulse rate below 30pps. These changes were evident across bolus types and volumes. Given the impact on diagnostic accuracy and the low radiation risks to adults undergoing MBSSs, reducing pulse rate to 15pps or below is not aligned with the As Low As Reasonably Achievable (ALARA) principle and should not be used as a viable method to reduce radiation exposure from MBSSs.
Occult metastasis is no burden factor in oral squamous cell carcinoma patients when adhering to a standardized approach in neck dissection
Authors: Struckmeier Ann-Kristin , Buchbender Mayte , Moest Tobias , Lutz Rainer , Agaimy Abbas , Kesting Marco 
Published: January 2024

Abstract
Objectives
Management of the neck in patients with oral squamous cell carcinoma (OSCC) is pivotal to oncologic control and survival. However, there is controversy regarding necessity of neck dissection (ND) in patients with clinically node-negative neck. We aimed to assess risk factors for occult metastasis and to explore whether the presence of occult lymph node metastases (LNMs) has an impact on recurrence and survival.

Material and methods
A retrospective cohort study was performed including patients with primary OSCC who underwent radical tumor resection and ND in a high-volume center adhering to the prevailing German guideline. The ND was performed according to a standardized approach.

Results
Four hundred twenty-one patients with primary surgically treated OSCC were included. The incidence of occult metastasis was 14.49%. A pathological T stage > 1 (multivariate analysis, odds ratio (OR) 3.958, p = 0.042) and the presence of extranodal extension in LNMs (multivariate analysis, OR 0.287, p = 0.020) were identified as independent risk factors for occult metastasis. When comparing patients with and without occult metastasis, there were no significant differences in terms of progression-free survival (log-rank, p = 0.297) and overall survival (log-rank, p = 0.320). There were no cases of ipsilateral neck recurrence. One patient developed contralateral neck metastasis; however, he initially presented with a unilateral pT1 pN0 tumor.

Conclusions
Overall, our findings suggest that conducting a standardized approach in ND should be applied in terms of management of the neck in order to maintain survival rates and to prevent neck recurrence in OSCC patients.
Clinical relevance.
None of the risk factors for occult metastasis can be reliably assessed preoperatively. Although elective ND does not guarantee the complete prevention of neck recurrence, it increases the likelihood of either timely removal of micrometastases or strengthens the justification for adjuvant therapy. Consequently, this approach leads to improvements in clinical outcomes.
Antecedent-contained argument ellipsis in Japanese
Authors: Takahashi Daiko 
Published: January 2024

AbstractThis article aims to provide new and solid evidence for the observation made in the previous literature that antecedent containment needs to be resolved in overt syntax in cases of antecedent contained deletion in Japanese. The evidence is new in the sense that it is based on argument ellipsis, which the recent literature has convincingly shown to be available in Japanese, rather than verb-stranding VP-ellipsis, the existence of which has been debated. It is shown how to construct relevant data using elliptic clausal arguments in the hope that similar research will be conducted with data from other languages where argument ellipsis is assumed to be available. It is argued that cases of antecedent-contained argument ellipsis in Japanese can be accounted for by the PF deletion analysis or the derivational copying analysis, but not by the LF copying analysis that posits that copying applies at the very end of the LF derivation.
Hybrid inorganic–organic coatings enhancing glass corrosion resistance
Authors: Pipota Tomáš , Peňáková Helena , Helebrant Aleš 
Published: January 2024

AbstractThis work aims to prepare a functional hybrid organic–inorganic coating on glass using the sol–gel process. The sol–gel coatings are commonly used for changing and/or enhancing optical, chemical and mechanical properties of the substrate glass. The coatings prepared in this work should exhibit high transmittance and good chemical resistance, whereof should be achieved by the combination of default precursor tetraethoxysilane (TEOS) and various functionalized silicon alkoxides 3-(trimethoxysilyl)propyl methacrylate (MEMO) and isobutyl(trimethoxy)silane (IBTMS). Sols were applied on substrate glass via the dip-coating method at different withdrawal speeds. Coatings were dried 1 h at 200 °C in ambient atmosphere. Assessment of transmittance was done by UV-Vis spectrophotometry, chemical resistance was observed by accelerated weathering tests in a humidity chamber, mechanical properties were evaluated with the means of standardized pencil hardness test and cross-cut tape test, and moreover analysis of abrasion resistance was done by model test. The most efficient functionalization was combination of 1% IBTMS + 1% MEMO and functionalization with MEMO up to 2% alone. These coatings showed good resistance against damp heat, they can withstand certain loads of abrasion and concurrently still exhibit high transmittance in the visible spectrum.
Graphical Abstract