bluuebunny/crossref_metadata_2025_split huggingface dataset:
Follow-up of intramyocardial bone marrow mononuclear cell transplantation beyond 10 years
Authors: Mulari Severi , Kesävuori Risto , Stewart Juhani A. , Karjalainen Pasi , Holmström Miia , Lehtinen Miia , Peltonen Juha , Laine Mika , Sinisalo Juha , Juvonen Tatu , Kupari Markku , Harjula Ari , Pätilä Tommi , Kivistö Sari , Kankuri Esko , Vento Antti ,   the Helsinki BMMC Collaboration, Hämmäinen Pekka , Schildt Jukka , Ahonen Aapo , Nikkinen Päivi , Nihtinen Anne , Alitalo Riitta , Pöyhiä Reino 
Published: February 2024

AbstractBone marrow mononuclear cells (BMMCs) have been evaluated for their ability to improve cardiac repair and benefit patients with severe ischemic heart disease and heart failure. In our single-center trial in 2006–2011 we demonstrated the safety and efficacy of BMMCs injected intramyocardially in conjunction with coronary artery bypass surgery. The effect persisted in the follow-up study 5 years later. In this study, we investigated the efficacy of BMMC therapy beyond 10 years. A total of 18 patients (46%) died during over 10-years follow-up and 21 were contacted for participation. Late gadolinium enhancement cardiac magnetic resonance imaging (CMRI) and clinical evaluation were performed on 14 patients, seven from each group. CMRIs from the study baseline, 1-year and 5-years follow-ups were re-analyzed to enable comparison. The CMRI demonstrated a 2.1-fold larger reduction in the mass of late gadolinium enhancement values between the preoperative and the over 10-years follow-up, suggesting less scar or fibrosis after BMMC treatment (− 15.1%; 95% CI − 23 to − 6.7% vs. − 7.3%; 95% CI − 16 to 4.5%, p = 0.039), compared to placebo. No differences in mortality or morbidity were observed. Intramyocardially injected BMMCs may exert long-term benefits in patients with ischemic heart failure. This deserves further evaluation in patients who have received BMMCs in international clinical studies over two decades.
Impulsivity and aggression as risk factors for internet gaming disorder among university students
Authors: Hammad Mohammad Ahmed , AL-shahrani Hend Faye 
Published: February 2024

AbstractInternet gaming addiction is a global problem, especially among young individuals. Exhibiting characteristics similar to other addictions, Internet Gaming Disorder (IGD) is linked to adverse mental health outcomes. Identified as risk factors for dependence behaviors, the association of impulsivity and aggression with IGD is relatively under-researched in the student population. The present sample of 350 university students (Mage = 21.30 years, SDage = 4.96 years) from Najran university in Saudi Arabia completed an online questionnaire that included the Internet Gaming Disorder Scale–Short-Form (IGDS9-SF), the Buss–Perry Aggression Questionnaire-Short Form, and the Barratt Impulsiveness Scale (BIS-15). Results indicated that impulsivity and aggression were positively associated with IGD severity and both personality traits explained 34.6% of the variance in IGD scores. Further bivariate analyses suggested that individuals spending 7 or more hours on internet gaming were more likely to exhibit high impulsivity and aggression, and had a relatively higher severity of IGD. These results suggest that individuals with these personality traits may be more vulnerable to developing an addiction to internet gaming. These findings need to be confirmed in future more robust studies; however, this exploratory study provides insights for potential programs to prevent IGD among young individuals.
SARS-CoV-2 infection status in corneal preservation solution and COVID-19 prevalence after corneal transplantation
Authors: Wasai Yuki , Yamada Naoyuki , Ariyoshi Nobuaki , Haraguchi Aiko , Funatsu Masahiko , Mikuni Masanori , Nakamura Riku , Sakuma Ayano , Higashijima Fumiaki , Iwamoto Nanako , Teranishi Shinichiro , Nishioka Mitsuaki , Yamasaki Takahiro , Kimura Kazuhiro 
Published: February 2024

AbstractThe potential risks associated with organs from COVID-19-infected donors were unclear. To determine the SARS-CoV-2 infection status of corneas transplanted during the COVID-19 pandemic, we performed a polymerase chain reaction (PCR) using the corneal preservation solution that was used for corneal transplantation. We also examined the postoperative health status of the recipients. This study included 144 transplants in 143 eyes. Ninety-nine eyes of imported corneas and 10 of the 14 corneas donated in the prefecture were PCR tested at our hospital, and all were SARS-CoV-2 negative. All corneal transplants were performed after confirming their SARS-CoV-2 negativity by a PCR using a corneal preservation solution at our hospital or a nasopharyngeal swab at a previous facility. Despite postoperative steroid administration, no patient developed COVID-19 infection until discharge. Hence, if the donor's nasopharyngeal swab test is SARS-CoV-2 negative, COVID-19 infection in the recipient due to corneal transplantation may be prevented. Since corneal transplant recipients are susceptible to infection due to prolonged steroid administration and are at high risk for severe diseases if infection occurs, SARS-CoV-2 detection testing using nasopharyngeal swabs in donors should be performed.
An inflamed tumor cell subpopulation promotes chemotherapy resistance in triple negative breast cancer
Authors: Jacobo Jacobo Mauricio , Donnella Hayley J. , Sobti Sushil , Kaushik Swati , Goga Andrei , Bandyopadhyay Sourav 
Published: February 2024

AbstractIndividual cancers are composed of heterogeneous tumor cells with distinct phenotypes and genotypes, with triple negative breast cancers (TNBC) demonstrating the most heterogeneity among breast cancer types. Variability in transcriptional phenotypes could meaningfully limit the efficacy of monotherapies and fuel drug resistance, although to an unknown extent. To determine if transcriptional differences between tumor cells lead to differential drug responses we performed single cell RNA-seq on cell line and PDX models of breast cancer revealing cell subpopulations in states associated with resistance to standard-of-care therapies. We found that TNBC models contained a subpopulation in an inflamed cellular state, often also present in human breast cancer samples. Inflamed cells display evidence of heightened cGAS/STING signaling which we demonstrate is sufficient to cause tumor cell resistance to chemotherapy. Accordingly, inflamed cells were enriched in human tumors taken after neoadjuvant chemotherapy and associated with early recurrence, highlighting the potential for diverse tumor cell states to promote drug resistance.
Life cycle assessment of nutrient recovery strategies from domestic wastewaters to quantify environmental performance and identification of trade-offs
Authors: Pausta Carla Mae , Kalbar Pradip , Saroj Devendra 
Published: February 2024

AbstractIncrease in anthropogenic activities proliferated the consumption of resources such as phosphorus; and increase the adverse environmental impacts especially eutrophication on water resources such as lakes. Nutrient recovery from domestic wastewaters to produce a fertiliser has been explored to address these challenges in the context of a sustainable circular nutrient economy. Life cycle assessment (LCA) was performed to holistically assess the impacts of integrating a nutrient recovery system on wastewater and water resource management using Laguna de Bay, Philippines as the geographical boundary. The inventory was developed based on the results of the emerging nutrient recovery reactor operations and the application of the recovered fertiliser on the agricultural crops. The LCA results for the proposed scenario showed environmental benefits of about 83.6% freshwater eutrophication, 102.5% terrestrial ecotoxicity, 26.9% water consumption, 100.7% mineral resource scarcity, while the global warming potential is 95.4% higher than the baseline scenario. Results imply policy review for septage management, system optimisation, and evaluation of alternative methods of wastewater management, in terms of life cycle thinking and sustainability across the globe.
Diversity of selected toll-like receptor genes in cheetahs (Acinonyx jubatus) and African leopards (Panthera pardus pardus)
Authors: Meißner René , Mokgokong Prudent , Pretorius Chantelle , Winter Sven , Labuschagne Kim , Kotze Antoinette , Prost Stefan , Horin Petr , Dalton Desire , Burger Pamela A. 
Published: February 2024

AbstractThe anthropogenic impact on wildlife is ever increasing. With shrinking habitats, wild populations are being pushed to co-exist in proximity to humans leading to an increased threat of infectious diseases. Therefore, understanding the immune system of a species is key to assess its resilience in a changing environment. The innate immune system (IIS) is the body’s first line of defense against pathogens. High variability in IIS genes, like toll-like receptor (TLR) genes, appears to be associated with resistance to infectious diseases. However, few studies have investigated diversity in TLR genes in vulnerable species for conservation. Large predators are threatened globally including leopards and cheetahs, both listed as 'vulnerable' by IUCN. To examine IIS diversity in these sympatric species, we used next-generation-sequencing to compare selected TLR genes in African leopards and cheetahs. Despite differences, both species show some TLR haplotype similarity. Historic cheetahs from all subspecies exhibit greater genetic diversity than modern Southern African cheetahs. The diversity in investigated TLR genes is lower in modern Southern African cheetahs than in African leopards. Compared to historic cheetah data and other subspecies, a more recent population decline might explain the observed genetic impoverishment of TLR genes in modern Southern African cheetahs. However, this may not yet impact the health of this cheetah subspecies.
RiceProteomeDB (RPDB): a user-friendly database for proteomics data storage, retrieval, and analysis
Authors: Woo Dong U , Lee Yejin , Min Cheol Woo , Kim Sun Tae , Kang Yang Jae 
Published: February 2024

AbstractRice, feeding a significant portion of the world, poses unique proteomic challenges critical to agricultural research and global food security. The complexity of the rice proteome, influenced by various genetic and environmental factors, demands specialized analytical approaches for effective study. The central challenges in rice proteomics lie in developing custom methods suited to the unique aspects of rice biology. These include data preprocessing, method selection, and result validation, all of which are essential for advancing rice research. Our aim is to decode these proteomic intricacies to facilitate breakthroughs in strain improvement, disease resistance, and yield optimization, all vital for combating global food insecurity. To achieve this, we have created the RiceProteomeDB (RPDB), a React + Django database, offering a streamlined and comprehensive platform for the analysis of rice proteomics data. RiceProteomeDB (RPDB) simplifies proteomics data management and analysis. It offers features for data organization, preprocessing, method selection, result validation, and data sharing. Researchers can access processed rice proteomics data, conduct analyses, and explore experimental conditions. The user-friendly web interface enhances navigation and interaction. RPDB fosters collaboration by enabling data sharing and proper acknowledgment of sources, contributing to proteomics research and knowledge dissemination. Availability and implementation: Web application:http://riceproteome.plantprofile.net/. The web application’s source code, user’s manual, and sample data:https://github.com/dongu7610/Riceproteome.
Water intake, baseline biopsy, and graft function after living donor kidney transplantation
Authors: Yamanaga Shigeyoshi , Hidaka Yuji , Kawabata Chiaki , Toyoda Mariko , Tanaka Kosuke , Yamamoto Yasuhiro , Inadome Akito , Takeda Asami , Yokomizo Hiroshi 
Published: February 2024

AbstractIncreased water intake is recommended for kidney transplant recipients; however, its efficacy remains controversial. We hypothesized that pre-existing histological findings of the allograft might modulate the impact of water intake. We retrospectively analyzed 167 adults with living-donor kidney transplants (April 2011–May 2020; median observation period, 77 months) whose baseline biopsy data were available. We compared the chronic-change group (n = 38) with the control group (n = 129) to assess the impact of self-reported daily water intake on the estimated glomerular filtration rate (eGFR). The range distribution of water intake was as follows: − 1000 ml (n = 4), 1000–1500 ml (n = 23), 1500–2000 ml (n = 64), 2000–2500 ml (n = 57), 2500–3000 ml (n = 16), and 3000 − ml (n = 3). Donor age was significantly higher in the chronic-change group. In the control group, the ΔeGFR/year increase was correlated with water intake. However, the increase in the water intake of the chronic-change group significantly decreased ΔeGFR/year (1000–1500 ml: + 1.95 ml/min/1.73 m2 and > 2000 ml: − 1.92 ml/min/1.73 m2, p = 0.014). This study suggested a potential influence of increased water intake on recipients with marginal grafts in living donor kidney transplantation.
Corticostriatal activity related to performance during continuous de novo motor learning
Authors: Park Sungbeen , Kim Junghyun , Kim Sungshin 
Published: February 2024

AbstractCorticostriatal regions play a pivotal role in visuomotor learning. However, less research has been done on how fMRI activity in their subregions is related to task performance, which is provided as visual feedback during motor learning. To address this, we conducted an fMRI experiment in which participants acquired a complex de novo motor skill using continuous or binary visual feedback related to performance. We found a highly selective response related to performance in the entire striatum in both conditions and a relatively higher response in the caudate nucleus for the binary feedback condition. However, the ventromedial prefrontal cortex (vmPFC) response was significant only for the continuous feedback condition. Furthermore, we also found functional distinction of the striatal subregions in random versus goal-directed motor control. These findings underscore the substantial effects of the visual feedback indicating performance on distinct corticostriatal responses, thereby elucidating its significance in reinforcement-based motor learning.
Automated sepsis detection with vancomycin- and allantoin-polydopamine magnetic nanoparticles
Authors: Abafogi Abdurhaman Teyib , Lee Jinyeop , Kim Joochan , Lee Sei Won , Jang Seongsoo , Park Sungsu 
Published: February 2024

AbstractRapid and accurate identification of the bacteria responsible for sepsis is paramount for effective patient care. Molecular diagnostic methods, such as polymerase chain reaction (PCR), encounter challenges in sepsis due to inhibitory compounds in the blood, necessitating their removal for precise analysis. In this study we present an innovative approach that utilizes vancomycin (Van) and allantoin (Al)-conjugated polydopamine (PDA)-coated magnetic nanoparticles (MNPs) for the rapid and automated enrichment of bacteria and their DNA extraction from blood without inducing clumping and aggregation of blood. Al/Van-PDA-MNPs, facilitated by IMS, eliminate the need for preliminary sample treatments, providing a swift and efficient method for bacterial concentration and DNA extraction within an hour. Employing Al/Van-PDA-MNPs within an automated framework has markedly improved our ability to pre-concentrate various Gram-negative and Gram-positive bacteria directly from blood samples. This advancement has effectively reduced the detection threshold to 102 colony-forming unit/mL by both PCR and quantitative PCR. The method's expedited processing time, combined with its precision, positions it as a feasible diagnostic tool for diverse healthcare settings, ranging from small clinics to large hospitals. Furthermore, the innovative application of nanoparticles for DNA extraction holds promising potential for advancing sepsis diagnostics, enabling earlier interventions and improving patient outcomes.
Time and frequency analysis of daily-based nexus between global CO2 emissions and electricity generation nexus by novel WLMC approach
Authors: Kartal Mustafa Tevfik , Ulussever Talat , Pata Ugur Korkut , Depren Serpil Kılıç 
Published: February 2024

AbstractThe studies have focused on changes in CO2 emissions over different periods, including the COVID-19 pandemic. Even if CO2 emissions are temporarily reduced during the pandemic according to annual figures, this may be misleading. Considering annual figures is important to understand the overall trend, but using data with much higher frequency (e.g., daily) is much better suited to investigate dynamic relationships and external effects. Therefore, this study comprehensively analyzes the association between CO2 emissions and disaggregated electricity generation (EG) sources across the globe by employing the novel wavelet local multiple correlation (WLMC) approach on daily data from 1st January 2020 to 31st March 2023. The results demonstrate that (1) based on the main statistics, daily CO2 emissions range between 69 MtCO2 and 116 MtCO2, indicating that there is an oscillation, but no sharp changes over the analyzed period. (2) based on the baseline regression using the dynamic ordinary least squares (DOLS) approach, the constructed estimation models have a high predictive ability of CO2 emissions, reaching ~ 94%; (3) in the further analysis employing the WLMC approach, there are significant externalities between EG resources, which affect CO2 emissions. The results present novel insights about time- and frequency-varying effects as well as a disaggregated analysis of the effect of EG on CO2 emissions, demonstrating the significance of the energy transition towards clean sources around the world.
Vestibulo-ocular reflex dynamics with head-impulses discriminates Usher patients type 1 and 2
Authors: Amorim Ana Margarida , Ramada Ana Beatriz , Lopes Ana Cristina , Duarte Silva Eduardo , Lemos João , Ribeiro João Carlos 
Published: February 2024

AbstractUsher Syndrome classification takes into account the absence of vestibular function but its correlation with genotype is not well characterized. We intend to investigate whether video Head Impulse Test (vHIT) is useful in screening and to differentiate Usher Syndrome types. 29 Usher patients (USH) with a genetically confirmed diagnosis and 30 healthy controls were studied with vHIT and dizziness handicap inventory questionnaire (DHI). Statistical significant differences between USH1, USH2 and controls were found in the vestibulo-ocular-reflex (VOR) gain of all SCCs, with USH1 patients consistently presenting smaller gains. VOR gain of the right lateral SCC could discriminate controls from USH1, and USH2 from USH1 with an overall diagnostic accuracy of 90%. USH1 DHI correlated with VOR (ρ = − 0,971, p = 0.001). Occurrence rate of covert and overt lateral semicircular canals refixation saccades (RS) was significantly different between groups, being higher in USH1 patients (p < 0.001). USH1 peak velocity of covert and overt saccades was higher for lateral semicircular canals (p < 0.05 and p = 0.001) compared with USH2 and controls. Covert saccades occurrence rate for horizontal SCCs could discriminate USH1 from USH2 patients and controls with a diagnostic accuracy of 85%. vHIT is a fast and non-invasive instrument which allowed us to screen and distinguish Usher patients from controls with a high precision. Importantly, its use allowed further discrimination between USH1 from USH2 groups. Moreover, VOR gain seems to correlate with vertigo-related quality of life in more severe phenotypes.
The carbon emissions of writing and illustrating are lower for AI than for humans
Authors: Tomlinson Bill , Black Rebecca W. , Patterson Donald J. , Torrance Andrew W. 
Published: February 2024

AbstractAs AI systems proliferate, their greenhouse gas emissions are an increasingly important concern for human societies. In this article, we present a comparative analysis of the carbon emissions associated with AI systems (ChatGPT, BLOOM, DALL-E2, Midjourney) and human individuals performing equivalent writing and illustrating tasks. Our findings reveal that AI systems emit between 130 and 1500 times less CO2e per page of text generated compared to human writers, while AI illustration systems emit between 310 and 2900 times less CO2e per image than their human counterparts. Emissions analyses do not account for social impacts such as professional displacement, legality, and rebound effects. In addition, AI is not a substitute for all human tasks. Nevertheless, at present, the use of AI holds the potential to carry out several major activities at much lower emission levels than can humans.
Injury-induced cooperation of InhibinβA and JunB is essential for cell proliferation in Xenopus tadpole tail regeneration
Authors: Nakamura Makoto , Kyoda Tatsuya , Yoshida Hitoshi , Takebayashi-Suzuki Kimiko , Koike Ryota , Takahashi Eri , Moriyama Yuka , Wlizla Marcin , Horb Marko E. , Suzuki Atsushi 
Published: February 2024

AbstractIn animal species that have the capability of regenerating tissues and limbs, cell proliferation is enhanced after wound healing and is essential for the reconstruction of injured tissue. Although the ability to induce cell proliferation is a common feature of such species, the molecular mechanisms that regulate the transition from wound healing to regenerative cell proliferation remain unclear. Here, we show that upon injury, InhibinβA and JunB cooperatively function for this transition during Xenopus tadpole tail regeneration. We found that the expression of inhibin subunit beta A (inhba) and junB proto-oncogene (junb) is induced by injury-activated TGF-β/Smad and MEK/ERK signaling in regenerating tails. Similarly to junb knockout (KO) tadpoles, inhba KO tadpoles show a delay in tail regeneration, and inhba/junb double KO (DKO) tadpoles exhibit severe impairment of tail regeneration compared with either inhba KO or junb KO tadpoles. Importantly, this impairment is associated with a significant reduction of cell proliferation in regenerating tissue. Moreover, JunB regulates tail regeneration via FGF signaling, while InhibinβA likely acts through different mechanisms. These results demonstrate that the cooperation of injury-induced InhibinβA and JunB is critical for regenerative cell proliferation, which is necessary for re-outgrowth of regenerating Xenopus tadpole tails.
Activin is a neural inducer of a male-specific muscle in Drosophila
Authors: Kimura Ken-ichi , Kumano Rimi , Yamamoto Daisuke 
Published: February 2024

AbstractDrosophila melanogaster has a pair of male-specific muscles called the muscle of Lawrence (MOL) in abdominal segment 5 (A5) of adult flies. The MOL is produced only when its innervating motoneuron expresses FruitlessM (FruM) neural masculinizing proteins. We show that MOL induction is hampered by: (1) silencing electrical activities in the motoneuron, (2) blocking vesicular release from the motoneuron, and (3) knocking down Activin ß (Actß) in the motoneuron or knocking down Actß signaling pathway components in the myoblasts. Our timelapse live imaging of the developing neuromuscular system reveals that, upon contact with the presumptive MOL, the motoneuronal axon retracts concomitant with the progression of MOL degeneration resulting from neural silencing. We conclude that MOL formation depends on the bidirectional trophic interactions between pre- and postsynaptic cells, with motoneuron-derived Actß playing an inducing role in MOL formation.