bluuebunny/crossref_metadata_2025_split huggingface dataset:
NRP1-expressing monocyte/macrophages promote arteriogenesis in the ischemic limb
Authors: Cho JS , Patel AS , Fantin A , Ludwinski FE , Pichette CJ , Rudnicki M. , Denti L , Mondragon A , Saha P , Smith A , Ruhrberg C , Modarai B 
Published: February 2024

AbstractPeripheral arterial disease can cause limb threatening blood flow restriction, requiring amputation in up to a third of patients despite contemporary treatments. Stimulating new blood vessel growth in ischemic limbs using growth factors such as VEGF-A or supplying mixed cell populations via autologous transplantation has been investigated as a therapy for surgically intractable cases, but to date has proven ineffective in randomised controlled clinical studies. To identify more potent pro-arteriogenic cell populations, we have investigated VEGF-A responsive myeloid cell populations in a mouse model of severe hindlimb ischemia. We found that NRP1 ablation from monocytes and macrophages impaired arteriogenesis in the adductor muscle and flow recovery in the injured limb. Vice versa, the exogenous delivery of NRP1-expressing (but not NRP1-negative) macrophages enhanced arteriogenesis in the adductor and flow recovery in the ischemic limb in a VEGF signalling-dependent manner. Further, patient-derived NRP1-expressing monocytes promoted vascular morphogenesis ex vivo. As the levels of circulating NRP1-expressing monocytes were raised in patients with limb ischemia, the therapeutic delivery of autologous NRP1-expressing monocytes could be explored as a treatment for critical limb ischemia.
How to validate a Bayesian evolutionary model
Authors: Mendes Fábio K. , Bouckaert Remco , Carvalho Luiz M. , Drummond Alexei J. 
Published: February 2024

AbstractBiology has become a highly mathematical discipline in which probabilistic models play a central role. As a result, research in the biological sciences is now dependent on computational tools capable of carrying out complex analyses. These tools must be validated before they can be used, but what is understood as validation varies widely among methodological contributions. This may be a consequence of the still embryonic stage of the literature on statistical software validation for computational biology. Our manuscript aims to advance this literature. Here, we describe and illustrate good practices for assessing the correctness of a model implementation, with an emphasis on Bayesian methods. We also introduce a suite of functionalities for automating validation protocols. It is our hope that the guidelines presented here help sharpen the focus of discussions on (as well as elevate) expected standards of statistical software for biology.
A latent pool of neurons silenced by sensory-evoked inhibition can be recruited to enhance perception
Authors: Gauld Oliver M. , Packer Adam M. , Russell Lloyd E. , Dalgleish Henry W.P. , Iuga Maya , Sacadura Francisco , Roth Arnd , Clark Beverley A. , Häusser Michael 
Published: February 2024

SUMMARYWhich patterns of neural activity in sensory cortex are relevant for perceptual decision-making? To address this question, we used simultaneous two-photon calcium imaging and targeted two-photon optogenetics to probe barrel cortex activity during a perceptual discrimination task. Head-fixed mice discriminated bilateral whisker deflections and reported decisions by licking left or right. Two-photon calcium imaging revealed sparse coding of contralateral and ipsilateral whisker input in layer 2/3 while most neurons did not show task-related activity. Activating small groups of pyramidal neurons using two-photon holographic photostimulation evoked a perceptual bias that scaled with the number of neurons photostimulated. This effect was dominated by the optogenetic activation of a small number of non-coding neurons, which did not show sensory or motor-related activity during task performance. Patterned photostimulation also revealed potent recruitment of cortical inhibition during sensory processing, which strongly and preferentially suppressed non-coding neurons. Our results provide a novel perspective on the circuit basis for the sparse coding model of somatosensory processing in which a pool of non-coding neurons, selectively suppressed by strong network inhibition during whisker stimulation, can be recruited to enhance perception.HighlightsAll-optical interrogation of barrel cortex during bilateral whisker discriminationSparse coding of contralateral and ipsilateral whisker informationSelective sensory-evoked inhibition helps ensure sparse codingOptogenetic recruitment of stimulus non-coding neurons can aid perception
Zapit: Open Source Random-Access Photostimulation For Neuroscience
Authors: Lohse Michael , Gauld Oliver M , Skrętowska Maja T , Vincent Peter , Pajot-Moric Quentin , Townsend Simon , Duan Chunyu A , Akrami Athena , Mrsic-Flogel Thomas D , Campbell Robert AA 
Published: February 2024

AbstractOptogenetic tools are indispensable for understanding the causal neural mechanisms underlying animal behaviour. While optogenetic actuators provide millisecond-precision control over genetically defined neural populations, successful optogenetic experiments also critically depend on associated hardware for targeted light delivery. Optic-fibres are suitable for certain experiments, however fibre implantation can be invasive and limits flexibility of spatial targeting. In contrast, random-access laser-scanning optogenetic systems provide far greater flexibility for targeting distributed cortical areas. However, these systems can be technically challenging to build, and at present no open source solution is available.Here we present ‘Zapit’, a complete open source platform for spatio-temporally precise random-access laser-scanning optogenetic experiments in head-fixed mice. We describe the system, quantify its performance, and show results from proof of principle cortical photoinhibition experiments in behaving mice.
Amelioration of signalling deficits underlying metabolic shortfall in TREM2R47Hhuman iPSC-derived microglia
Authors: Vasilopoulou Foteini , Piers Thomas M. , Jingzhang Wei , Hardy John , Pocock Jennifer M. 
Published: February 2024

AbstractClassic immunometabolic switching from oxidative phosphorylation (OXPHOS) to glycolysis occurs in myeloid cells such as microglia when encountering pathogenic insults. We previously showed that patient iPSC-derived microglia (iPS-Mg) harbouring the Alzheimer’s disease (AD) TREM2R47Hhypomorph display deficits in the ability to metabolically switch from OXPHOS to glycolysis, with both reduced mitochondrial maximal respiration and glycolytic capacity. To resolve this, we generated common variant, TREM2R47Hand TREM2−/−variant human iPS-Mg and we assessed the ability of supplementation with citrate or succinate, key metabolites and cell cycle breaking points upon microglia activation, to overcome deficits in a number of functions we identified previously as affected by TREM2 loss of function phenotypes. Succinate supplementation was more effective than citrate at overcoming mitochondrial deficits in OXPHOS and did not promote a switch to glycolysis. Citrate enhanced the lipid content of TREM2R47HiPS-Mg and was more effective at overcoming phagocytic deficits whereas succinate increased lipid content and phagocytic capacity in TREM2−/−iPS-Mg. Microglia cytokine release upon activation was differentially affected by citrate or succinate and neither metabolite altered soluble TREM2 shedding. Neither citrate nor succinate enhanced glycolysis but drove their effects through oxidative phosphorylation. Our data point to discrete pathway linkage between microglial metabolism and functional outcomes with implications for AD pathogenesis and treatments.
Leave or stay? Factors influencing consumers’ purchase intention during the transformation of a content anchor to a live stream anchor
Authors: Cao Cong , Chu Chengxiang , Ding Xinyi , Shi Yangyan 
Published: February 2024

PurposeAs live streaming becomes a widely used online sales mode, previously content-centred anchors are attempting to switch to e-commerce live streaming. The purpose of this research was to explore the mechanisms that prompt consumers to stay or leave after content anchors transfer to live e-commerce broadcasts. In addition, we explored the factors affecting consumption from the perspectives of anchors, consumers and the external environment.Design/methodology/approachWe distributed questionnaires to a group of fans who had experienced the transition of content anchors to live streaming and received back 375 valid questionnaires. Using psychological contract theory, we constructed a theoretical model for the scenario in which content anchors transition to live e-commerce broadcasting and analysed the data using partial least squares structural equation modelling (PLS-SEM).FindingsThe results show that circle culture, mainstream culture, initial trust and live streaming content all positively influenced consumers’ attitudes, whilst consumers’ past shopping experiences negatively influenced consumers’ attitudes. The personal charm of the content anchors did not have a significant effect on consumers’ attitudes. Additionally, we found that only anchors with a significant circle culture and good trust levels amongst fans were able to transition to live e-commerce streaming successfully.Originality/valueThis study extends the application of psychological contract theory to the field of e-commerce and describes the transformation of different types of psychological contracts. The paper’s conclusions provide a reference for decision-making and the implementation of transformation by content-based anchors to live streaming, helping them to coordinate their relationships with fans more effectively.
An optimization model for just-in-time (JIT) delivery of precast components considering 3D loading constraints, real-time road conditions and assembly time
Authors: Mao Wenqi , Ran Kexin , Wang Ting-Kwei , Yu Anyuan , Lv Hongyue , Chen Jieh-Haur 
Published: February 2024

PurposeAlthough extensive research has been conducted on precast production, irregular component loading constraints have received little attention, resulting in limitations for transportation cost optimization. Traditional irregular component loading methods are based on past performance, which frequently wastes vehicle space. Additionally, real-time road conditions, precast component assembly times, and delivery vehicle waiting times due to equipment constraints at the construction site affect transportation time and overall transportation costs. Therefore, this paper aims to provide an optimization model for Just-In-Time (JIT) delivery of precast components considering 3D loading constraints, real-time road conditions and assembly time.Design/methodology/approachIn order to propose a JIT (just-in-time) delivery optimization model, the effects of the sizes of irregular precast components, the assembly time, and the loading methods are considered in the 3D loading constraint model. In addition, for JIT delivery, incorporating real-time road conditions in the transportation process is essential to mitigate delays in the delivery of precast components. The 3D precast component loading problem is solved by using a hybrid genetic algorithm which mixes the genetic algorithm and the simulated annealing algorithm.FindingsA real case study was used to validate the JIT delivery optimization model. The results indicated this study contributes to the optimization of strategies for loading irregular precast components and the reduction of transportation costs by 5.38%.Originality/valueThis study establishes a JIT delivery optimization model with the aim of reducing transportation costs by considering 3D loading constraints, real-time road conditions and assembly time. The irregular precast component is simplified into 3D bounding box and loaded with three-space division heuristic packing algorithm. In addition, the hybrid algorithm mixing the genetic algorithm and the simulated annealing algorithm is to solve the 3D container loading problem, which provides both global search capability and the ability to perform local searching. The JIT delivery optimization model can provide decision-makers with a more comprehensive and economical strategy for loading and transporting irregular precast components.
Developing a sense of belonging among ethnic minority youth in Hong Kong: challenges and opportunities
Authors: Hue Ming Tak , Karim Shahid 
Published: February 2024

PurposeDeveloping a sense of belonging among immigrant youth in multicultural contexts has attracted significant attention from scholars during the last few decades. Studies have already underscored how various educational factors hinder or facilitate students’ sense of belonging to the school or the larger society. Although most students in Hong Kong schools are ethnic Chinese, a significant number of non-Chinese children make students diversity an essential aspect of schooling. The study investigated how schools can develop a sense of belonging among ethnic minority youth in Hong Kong.Design/methodology/approachAs the education system in Hong Kong lacks a multicultural education policy, how can schools help develop a sense of belonging to the school and the larger society among young ethnic minority people? To answer this question, this paper consolidates the two sets of data originally gathered for two research projects. The data was collected through semi-structured in-depth interviews with nine secondary school teachers (Chinese and non-Chinese) and 15 students (non-Chinese) and analysed thematically.FindingsThe thematic analysis of the qualitative data identified several challenges and opportunities for developing ethnic minority students’ sense of belonging in Hong Kong.Research limitations/implicationsResearchers in comparative education can further explore how multicultural education and inclusive education approach together can help ensure inclusive and equitable quality education for all and cater to students' diverse learning needs across the education systems.Practical implicationsGiven that the aims of multicultural education and inclusive education resonate with each other, schools can focus on the Whole School Approach to developing a sense of belonging among ethnic minority youth in Hong Kong. However, policymakers and practitioners may need to adopt a multifaceted perspective on inclusive education that strives to ensure equitable quality education for all.Originality/valueThe study contributes to the existing body of scholarship on multicultural education and inclusive education. The study findings underscore the importance of an interdisciplinary research framework in education and advocate an integrative approach to supporting students with diverse learning needs in multicultural contexts.
Unleashing the power of organizational social capital: exploring the mediating role of social entrepreneurship orientation in social enterprises' performances
Authors: Loukopoulos Argyrios , Papadimitriou Dimitra , Glaveli Niki 
Published: February 2024

PurposeThis study investigates the influence of organizational social capital (OSC) on the social and economic performance of social enterprises (SEs) in Greece and the mediating role of social entrepreneurship orientation (SEO) in these relationships.Design/methodology/approachA theoretical framework was developed integrating resource-based theory, OSC theory and behavioral entrepreneurship theory. The data were collected from 345 Greek SEs and structural equation modeling (SEM) with bootstrap analysis was employed to estimate path coefficients.FindingsThis study shows that OSC positively impacts SEs’ social and economic performance, while SEO mediates only the relationship between OSC and SEs’ social performance. This research offers insights for scholars, practitioners and policymakers in social entrepreneurship by highlighting the significance of OSC and SEO.Originality/valueThis study contributes to the literature on SEs by integrating resource-based theory, OSC theory and behavioral entrepreneurship theory, presenting a novel comprehensive theoretical framework for understanding SEs’ performances. Additionally, the study advances the understanding of SEO as a mediator in the relationship between OSC and SEs’ social and economic performance. The unique focus on the Greek context provides a valuable setting for examining the relationships among OSC, SEO and SEs’ performances.
Research on tourists' travel intention and usage of VR technologies based on SOR model: mediating role of personal values
Authors: Jiang Chunyu , Phoong Seuk Wai 
Published: February 2024

PurposeThis study investigated the travel intentions (TIs) of Chinese travelers and their utilization of virtual tourism technology during the Zero COVID-19 policy period by using a stimulus-organism-response (SOR) model. The study specifically examines the interplay between knowledge of policy, perception of risk, TI, usage of virtual tourism technology (UVTT) and the mediating role of personal values.Design/methodology/approachThe data were collected from 333 Chinese travelers through an online questionnaire, and structural equation modeling (SEM) was employed to test the proposed hypotheses.FindingsThe study suggests that knowledge of policy and risk perception increase changes in personal values (PVs), which, in turn, affect Chinese travelers' TIs and the UVTT, with PVs playing a mediating role. Risk perception has a positive effect on the UVTT.Practical implicationsThis study highlights the positive impact of tourism policy knowledge and risk awareness on individual values as a stimulus. Stakeholders need to implement industry-specific policies that are in line with scientific developments. Tourism managers should prioritize understanding the psychological reactions of tourists in crises and provide support to mitigate negative emotions. Anticipating changes in PVs is crucial, as instability affects tourists' behavior. The findings of the study also provide valuable insights for technology designers and underscore the substitutability of virtual technologies in improving the tourism experience.Originality/valueThis study is the first to examine the mediating role of PVs in the relationship between knowledge of policy, tourism risk perception (TRP), TI and the UVTT based on the SOR model. The insights gained from this analysis can assist policymakers and tourism managers in understanding the psychological changes of tourists, thereby facilitating the development of appropriate tourism planning.
Interorganizational networks in dynamics of disaster: Comprehensive approach to network effectiveness
Authors: Chang Saemi 
Published: February 2024

AbstractGovernments grapple with the challenges of uncertainty and complexity in disasters due to interconnected actors. This research explores how government organizations can effectively operate during disasters. Two aspects of network effectiveness—structural and procedural approaches—are considered. The structural approach emphasizes the network stability, while the procedural approach focuses on the process of network evolution. Analysing and comparing response networks from two earthquakes in Korea, a crucial environmental change—government reorganization—between them was identified. This allowed to assess how environmental changes impact network effectiveness. The findings suggest that effective disaster management requires complementing centralized authority with actual power distribution, empowering subnetwork groups as centres of common knowledge base, and emphasizing coordination among organizations with diverse expertise.
The renewable energy challenge in developing economies: An investigation of environmental taxation, financial development, and political stability
Authors: Rehman Abdul , Batool Zakia , Ain Qurat Ul , Ma Hengyun 
Published: February 2024

AbstractThe phenomenon of accelerated economic growth and heightened energy consumption in emerging economies is resulting in a substantial upsurge in CO2 emissions. Due to the inescapable apprehensions around climate change and the diminishing availability of fossil fuel resources, there exists a pervasive need on a worldwide scale to transition towards renewable energy sources. However, emerging economies have unique hurdles in adopting renewable energy technologies, owing to low financial resources, volatile political environments, and insufficient regulatory frameworks. Therefore, the present study aims to examine the effectiveness of financial development, environmental taxation, and political stability in facilitating the adoption of renewable energy in developing countries. The system‐Generalized Method of Moments approach was used to analyze the dataset including 17 emerging economies spanning the period from 1994 to 2021. The study also utilizes the cross‐sectional autoregressive distributed lag approach, and the results obtained from cross‐sectional autoregressive distributed lag align closely with those obtained from the system‐Generalized Method of Moments estimates, thereby confirming the reliability and strength of the findings. Results show that environmental taxes encourage the adoption of renewable energy; however, political stability and financial development help to provide a feasible structure for the adoption of renewable energy. The findings will contribute to the development of viable policy recommendations for these economies to facilitate the advancement of sustainable energy transitions.
Electrospun nanofibers based on zein and red onion bulb extract (Allium cepa, L.): Volatile compounds, hydrophilicity, and antioxidant activity
Authors: Cruz Elder Pacheco da , Pires Juliani Buchveitz , Jansen Estefani Tavares , Santos Felipe Nardo dos , Fonseca Laura Martins , Hackbart Helen Cristina dos Santos , Radünz Marjana , Zavareze Elessandra da Rosa , Dias Alvaro Renato Guerra 
Published: February 2024

AbstractOnion is rich in bioactive and volatile compounds with antioxidant activity. However, the pungent odor of volatile compounds (VOCs) released restricts its use. The encapsulation of red onion extract by electrospinning is an alternative to mask this odor and protect its bioactive compounds. The main objective of this study was to encapsulate red onion bulb extract (ROE) in different concentrations into zein nanofibers by electrospinning and evaluate their thermal, antioxidant, and hydrophilicity properties. The major VOC in ROE was 3(2H)‐furanone, 2‐hexyl‐5‐methyl. Incorporating ROE into the polymeric solutions increased electrical conductivity and decreased apparent viscosity, rendering nanofibers with a lower average diameter. The loading capacity of ROE on fibers was high, reaching 91.5% (10% ROE). The morphology of the nanofibers was random and continuous; however, it showed beads at the highest ROE concentration (40%). The addition of ROE to the nanofibers increased their hydrophilicity. The nanofibers’ antioxidant activity against 2,2‐diphenyl‐1‐picrylhydrazyl, nitric oxide, and hydroxyl radicals ranged from 32.5% to 57.3%. The electrospun nanofibers have the potential to protect and mask VOCs. In addition, they offer a sustainable alternative to the synthetic antioxidants commonly employed in the food and packaging industry due to their antioxidant activities.
Recent advances in the use of composite titanium dioxide nanomaterials in the food industry
Authors: Mao Xixi , Hao Changlong 
Published: February 2024

AbstractTitanium dioxide (TiO2) nanomaterials have attracted significant attention due to their good biocompatibility and potential for multifunctional applications. In the last few years, there has been growing interest in the use of TiO2 nanomaterials in the food industry. However, a systematic review of the synthesis methods, properties, and applications of TiO2 nanomaterials in the food industry is lacking. In this review, we provide a summary of the synthesis and properties of TiO2 nanomaterials and their composites, with a focus on their applications in the food industry. We also discuss the potential benefits and risks of using TiO2 nanomaterials in food applications. This review aims to promote food innovation and improve food quality and safety.