bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streptomycesextracellular vesicles are a broad and permissive antimicrobial packaging and delivery system
Authors: Meyer Kirsten J. , Nodwell Justin R. 
Published: October 2023

ABSTRACTStreptomycesare the primary source of bioactive specialized metabolites used in research and medicine, including many antimicrobials. These are presumed to be secreted and function as freely soluble compounds. However, increasing evidence suggests extracellular vesicles are an alternative secretion system. We assessed environmental and lab-adaptedStreptomyces(sporulating filamentous actinomycetes) and found a majority of strains produce vesicles packaging antimicrobial compounds. The cargo included actinomycins, anthracyclines, candicidin, and actinorhodin, reflecting both diverse chemical properties and diverse antibacterial and antifungal activity. The production of the antimicrobials and vesicles was linked, although vesicle production continued in the absence of antimicrobials. We demonstrated that antimicrobial-containing vesicles achieve direct delivery of the cargo to other microbes. Notably, this delivery via membrane fusion occurred to a broad range of microbes including pathogenic bacteria and yeast. Vesicle encapsulation offers a broad and permissive packaging and delivery system for antimicrobial specialized metabolites, with important implications for ecology and translation.GRAPHICAL ABSTRACTIMPORTANCEExtracellular vesicle encapsulation changes our picture of how antimicrobial metabolites function in the environment, and provides an alternative translational approach for delivery of antimicrobials. We find the majority ofStreptomycesstrains are capable of releasing antimicrobial vesicles, and at least four distinct classes of compounds can be packaged, suggesting this is widespread in nature. This is a striking departure from the primary paradigm of secretion and action of specialized metabolites as soluble compounds. Importantly the vesicles deliver antimicrobial metabolites directly to other microbes via membrane fusion, including to pathogenic bacteria and yeast. This suggests future applications in which lipid-encapsulated natural product antibiotics and antifungals could be used to solve some of the most pressing problems in drug resistance.
PyDecNef: An open-source framework for fMRI-based decoded neurofeedback
Authors: Margolles Pedro , Mei Ning , Elosegi Patxi , Soto David 
Published: October 2023

AbstractReal time fMRI research has suffered from inaccessible analysis pipelines, hindering collaboration and reproducibility. Here we present PyDecNef, a Python-based platform designed to advance real-time fMRI analysis and fuel exploration of close-loop neuroimaging for cognitive neuroscience studies. Creating a real-time fMRI analysis pipeline from scratch poses formidable technical challenges, involving data transfer, experimental software, and machine learning classifier preparation. Existing tools like FRIEND, Brain-Voyant, and OpenNFT demand expensive licenses or rely on proprietary software, impeding accessibility and customizability. PyDecNef offers a solution: a transparent, versatile, and open workflow for real-time fMRI decoding protocols. This open-source platform simplifies decoder construction, real-time preprocessing, decoding, and feedback signal generation. It empowers researchers to launch DecNef experiments efficiently, saving time and resources. Moreover, its openness promotes collaboration, enhancing research quality, replicability, and impact. With PyDecNef, the path to advancing DecNef studies becomes more accessible and collaborative. PyDecNef resources for real-time fMRI analysis can be found athttps://pedromargolles.github.io/pyDecNef/,. Here we also provide experimental data illustrating that PyDecNef provides more fine-grained and less binomial/overconfident neurofeedback signals compared to previous DecNef approaches that have relied on sparse multinomial logistic regression classifiers, and hence, potentially helping participants to learn better how to self-regulate their brain activity.
The macronuclear genomic landscape withinTetrahymena thermophila
Authors: Derelle Romain , Verdonck Rik , Jacob Staffan , Huet Michèle , Akerman Ildem , Philippe Hervé , Legrand Delphine 
Published: October 2023

AbstractThe extent of intraspecific genomic variation is key to understanding species evolutionary history, including recent adaptive shifts. Intraspecific genomic variation remains poorly explored in eukaryotic microorganisms, especially in the nuclear dimorphic ciliates, despite their fundamental role as laboratory model systems and their ecological importance in many ecosystems. We sequenced the macronuclear genome of 22 laboratory strains of the oligohymenophoranTetrahymena thermophila, a model species in both cellular biology and evolutionary ecology. We explored polymorphisms at the junctions of programmed eliminated sequences, and reveal their utility to barcode very closely related cells. As for other species of the genusTetrahymena, we confirm micronuclear centromeres as gene diversification centres inT. thermophila, but also reveal a two-speed evolution in these regions. In the rest of the genome, we highlight recent diversification of genes encoding for extracellular proteins and cell adhesion. We discuss all these findings in relation with ciliate’s ecology and cellular characteristics.Impact StatementThis is the first study of population genomics in the ciliateTetrahymena thermophila. This bacterivore species plays an important role in aquatic trophic chains and is widely used as a model in cell and molecular biology, ecology, evolution or toxicology. As all ciliates, it contains a germline micronucleus and a somatic macronucleus. Sequencing of the macronucleus reveals that the centromeric region of the micronucleus are simultaneously a region of new gene diversification, as observed in otherTetrahymenaspecies, and a region containing highly conserved genes. The results also confirm that the formation of the macronucleus from the micronucleus is highly imprecise. Interestingly, this process generates a genomic barcode that can discriminate cells derived from a given sexual reproduction event, allowing to study more finely population dynamics/history in nature.Data summaryAll data are fully provided in Supplementary Materials. The raw data of the 22Tetrahymenagenomes have been deposited in the Sequence Read Archive (https://www-ncbi-nlm-nih-gov.inee.bib.cnrs.fr/bioproject/PRJNA1012331). Accession numbers are listed in Table S1 (available in the online version of this article).
ExoChew: An exonuclease technique to generate single-stranded DNA libraries
Authors: Patel Krishna , Lodha Chirag , Smith Christopher , Diggins Levi , Kolluru Venkata , Ross Daniel , Syed Christopher , Lewis Olivia , Daley Rachel , Mohanty Bidyut K 
Published: October 2023

AbstractAlthough DNA in the genome is double-stranded, single-stranded DNA is generated during various processes including DNA replication and repair. Some single-stranded DNAs can form noncanonical structures. Various proteins bind to the single-stranded DNAs site-specifically and/or structure-specifically to regulate various DNA transactions. Because of the transient nature of single-stranded DNAs in the cell, currentin vivotechniques may not reveal all such sequences, structures, and protein-DNA complexes. To explore such sequences and structures genome-wide, it is necessary to generate single-stranded DNA libraries. Currentin vitromethods involve heat denaturation of libraries of double-stranded DNA fragments followed by cooling to prevent reannealing; however, a significant amount of DNA can reanneal to regenerate double-stranded DNAs. In ExoChew method, double-stranded DNA fragment libraries are enzymatically converted to single-stranded DNA libraries. Genomic DNA is sonicated to generate pools of double-stranded DNA fragments of required size. Each pool of double-stranded DNA fragments is then treated with either T7 exonuclease orE. coliexonuclease III. which recognize and cleave double-stranded DNA from 5’ ends or 3’ ends, generating single-stranded DNA pools, respectively. The enzymatically generated single-stranded DNA pools can be used for genome-wide studies of protein-DNA interactions and structural studies of DNA.
Antimicrobial use in laboratory rodent facilities in Australia and New Zealand- a cross-sectional survey of veterinarians and facility managers
Authors: Wilcox Rebbecca S. , Marenda Marc S. , Devlin Joanne M. , Wilks Colin R. 
Published: October 2023

AbstractThis cross-sectional study surveyed veterinarians and facility managers to characterise the use of antimicrobials in laboratory rodent facilities within Australia and New Zealand. Most facilities (71%) reported routine administration of antimicrobials. The indications for antibiotic use reflected those described in publications and differed significantly to reasons for use in non-laboratory animals. Antimicrobials used include those of critical importance to human health, and access to these drugs is unregulated, as prescription-only classes are ordered through research catalogues, without human or veterinary physician prescriptions.The ways in which antimicrobials are used in Australian and New Zealand rodent facilities are likely contributing to antimicrobial resistance within rodent populations, particularly as they are largely administered in drinking water, risking subtherapeutic dosing. Much antimicrobial use reported is unnecessary and could be replaced with changes to husbandry and handling. The generation of resistance in both pathogenic and commensal microbes may also represent a work health and safety issue for humans working with these animals.Reported disposal of antimicrobials included discharge into wastewater, without inactivation, and some respondents reported disposal of substrate, or soiled bedding, nesting material, and disposable enrichment items, from treated animals and medicated feed into landfill, without prior inactivation. Environmental contamination with resistant microbes and antimicrobials is a significant driver of antimicrobial resistance. As such, significant opportunities exist to implement judicious and responsible use of antimicrobials within research rodent facilities in Australia and New Zealand, with a particular focus on instituting aseptic surgery, optimising dosing regimens, and inactivation of medicated water and substrate before disposal.
BMP signaling maintains auricular chondrocyte identity and prevents microtia development by inhibiting protein kinase A
Authors: Yang Ruichen , Chu Hongshang , Yue Hua , Mishina Yuji , Zhang Zhenlin , Liu Huijuan , Li Baojie 
Published: October 2023

AbstractElastic cartilage constitutes a major component of the external ear, which functions to guide sound to the middle and inner ears. Defects in auricle development cause congenital microtia, which affects hearing and appearance in patients. Mutations in several genes have been implicated in microtia development, yet, the pathogenesis of this disorder remains incompletely understood. Here, we show thatPrrx1genetically marks auricular chondrocytes in adult mice. Interestingly, BMP-Smad1/5/9 signaling in chondrocytes is increasingly activated from the proximal to distal segments of the ear, which is associated with a decrease in chondrocyte regenerative activity. Ablation ofBmpr1ain auricular chondrocytes led to chondrocyte atrophy and microtia development at the distal part. Transcriptome analysis revealed thatBmpr1adeficiency caused a switch from the chondrogenic program to the osteogenic program, accompanied by enhanced protein kinase A activation, likely through increased expression ofAdcy5/8. Inhibition of PKA blocked chondrocyte-to-osteoblast transformation and microtia development. Moreover, analysis of single-cell RNA-seq of human microtia samples uncovered enriched gene expression in the PKA pathway and chondrocyte-to-osteoblast transformation process. These findings suggest that auricle cartilage is actively maintained by BMP signaling, which maintains chondrocyte identity by suppressing osteogenic differentiation.
Assessing the high-quality development strategy of mineral resource enterprises
Authors: Cheng Jingyu , Wang Minxi , Wu Lilin , Li Xin 
Published: October 2023

Purpose
The purpose of this paper is to explore the high-quality development (HQD) strategy of Chinese mineral resource enterprises, which is important for Chinese mineral resource enterprises to improve the efficiency and benefit of resource utilization, reduce the intensity of resource and energy consumption and gradually form resource-saving and environment-friendly enterprises.

Design/methodology/approach
This study establishes an evaluation index system with four dimensions: economy, environment, society and management innovation. The entropy value method assigns weights to them and then uses the system dynamics (SD) model for case simulation.

Findings
The results of the SD simulation conclude that the fulfillment of social responsibility and the implementation of management innovation can accelerate the realization of HQD of mineral resource enterprises; profitability plays a crucial role in economic indicators; the improvement of energy-saving volume has the most significant impact on environmental benefits; the social contribution is the key element to measure social indicators; and the sales rate of core products has the most significant impact on the benefits of management innovation.

Originality/value
Based on the few studies on the evaluation of the development strategy of mineral resource enterprises, this study establishes an evaluation index system that considers the interactions between indicators, combines the entropy value method with SD and uses the SD model to comprehensively and systematically analyze the impact and degree of each factor on the HQD of mineral resource enterprises.
A knowledge graph of interlinking digital records: the case of the 1997 Korean financial crisis
Authors: Kim Haklae 
Published: October 2023

Purpose
Despite ongoing research into archival metadata standards, digital archives are unable to effectively represent records in their appropriate contexts. This study aims to propose a knowledge graph that depicts the diverse relationships between heterogeneous digital archive entities.

Design/methodology/approach
This study introduces and describes a method for applying knowledge graphs to digital archives in a step-by-step manner. It examines archival metadata standards, such as Records in Context Ontology (RiC-O), for characterising digital records; explains the process of data refinement, enrichment and reconciliation with examples; and demonstrates the use of knowledge graphs constructed using semantic queries.

Findings
This study introduced the 97imf.kr archive as a knowledge graph, enabling meaningful exploration of relationships within the archive’s records. This approach facilitated comprehensive record descriptions about different record entities. Applying archival ontologies with general-purpose vocabularies to digital records was advised to enhance metadata coherence and semantic search.

Originality/value
Most digital archives serviced in Korea are limited in the proper use of archival metadata standards. The contribution of this study is to propose a practical application of knowledge graph technology for linking and exploring digital records. This study details the process of collecting raw data on archives, data preprocessing and data enrichment, and demonstrates how to build a knowledge graph connected to external data. In particular, the knowledge graph of RiC-O vocabulary, Wikidata and Schema.org vocabulary and the semantic query using it can be applied to supplement keyword search in conventional digital archives.
Determining cybersecurity culture maturity and deriving verifiable improvement measures
Authors: Dornheim Peter , Zarnekow Ruediger 
Published: October 2023

Purpose
The human factor is the most important defense asset against cyberattacks. To ensure that the human factor stays strong, a cybersecurity culture must be established and cultivated in a company to guide the attitudes and behaviors of employees. Many cybersecurity culture frameworks exist; however, their practical application is difficult. This paper aims to demonstrate how an established framework can be applied to determine and improve the cybersecurity culture of a company.

Design/methodology/approach
Two surveys were conducted within eight months in the internal IT department of a global software company to analyze the cybersecurity culture and the applied improvement measures. Both surveys comprised the same 23 questions to measure cybersecurity culture according to six dimensions: cybersecurity accountability, cybersecurity commitment, cybersecurity necessity and importance, cybersecurity policy effectiveness, information usage perception and management buy-in.

Findings
Results demonstrate that cybersecurity culture maturity can be determined and improved if accurate measures are derived from the results of the survey. The first survey showed potential for improving the dimensions of cybersecurity accountability, cybersecurity commitment and cybersecurity policy effectiveness, while the second survey proved that these dimensions have been improved.

Originality/value
This paper proves that practical application of cybersecurity culture frameworks is possible if they are appropriately tailored to a given organization. In this regard, scientific research and practical application combine to offer real value to researchers and cybersecurity executives.
Demographic, experience, and organisational factors effect on local enforcement officers’ ethical integrity
Authors: Zakaria Nor Balkish , Nordin Muhammad Farhan , Ismail Allezawati , Ahmad Shukri Nurul Huda , Baykal Elif 
Published: October 2023

Purpose
This study departed from the aim to progress Malaysia as a high-income nation in 2025 via decent work and economic growth (Sustainable Development Goal 8). Thus, this study aims to examine the effects of demographic, experience and organisational factors on the ethical integrity of local enforcement officers from self-proclaim and colleague perception perspectives.

Design/methodology/approach
The data of this study was collected from Pusat Latihan Penguatkuasa Selangor (PULAPES), a training centre for local enforcement officers in Selangor. Based on a survey in 2019, this study used primary data based on a scenario-based questionnaire survey with a total sample of 535 respondents.

Findings
From a self-proclaim perspective, the results show that secondment and training factors have a positive relationship with the ethical integrity of local enforcement officers. From a colleague perception perspective, the results indicate that the secondment factor has a positive relationship with ethical integrity. In contrast, the officer rank factor has a negative relationship with the ethical integrity of local enforcement officers.

Practical implications
This research seeks to develop new theories or refine existing ones to explain how diverse circumstances affect law enforcement ethics. Learning people’s habits through observation and consequences like rewards or punishments impact behaviour recurrence are suggested. Law enforcement ethics can be examined by examining how peers, supervisors and organisational culture shape officers’ ethics.

Social implications
The finding of this study could serve to evaluate training programmes or rewards and punishments for ethical behaviour including how accountability and community involvement aid to promote law enforcement ethics.

Originality/value
The survey results of this study are based on local enforcement officers’ ethics that serve to aid in illuminating the elements which affect ethical behaviour among law enforcement personnel and identify the tactics for fostering ethical behaviour.
Can insurance ensure economic growth in an emerging economy? Fresh evidence from a non-linear ARDL approach
Authors: Tran Quang Thien , Huynh Nhan 
Published: October 2023

Purpose
This study aims to explore the nexus between insurance penetration and economic development in Vietnam, one of the fastest-growing economies over the past two decades.

Design/methodology/approach
This study uses an updated data set of the insurance sector in Vietnam from 1996 to 2020. The autoregressive lagging distribution and cointegrating non-linear autoregressive lagging distribution (NARDL) models are used to explore the nexus between the insurance market development and economic growth.

Findings
This study confirms the unidirectional causality and positive impacts of insurance market development on economic growth both in the short and long term, supporting the “supply-leading” hypothesis. Nonlife insurance has more significant but slower impacts on contributing to economic development in the long run. From the NARDL approach, this study also discloses the asymmetric relationship between the insurance industry and economic growth. Aggregate and life insurance display short- and long-term asymmetric impacts, whereas nonlife insurance shows long-term asymmetry.

Originality/value
To the best of the authors’ knowledge, this is the first study to examine the hidden asymmetries of the insurance-growth nexus in Vietnam from non-linear models. Notwithstanding the theoretical contributions to the prior literature, several practical implications are proposed for insurance businesses, policymakers and investors.
Consumer confusion and negative eWOM in the e-hospitality marketplace: the moderated moderation model of gender and technology self-efficacy
Authors: Sharma Anuradha , Pandher Jagwinder Singh , Prakash Gyan 
Published: October 2023

PurposeThe goal of this paper is to use the stimulus-organism-response (S-O-R) paradigm to understand how ineffective marketplace stimuli affect perceptions related to online travel package booking, which in turn cultivate various types of confusion, and how these confusions are channelled into behavioural dispositions of consumers, such as negative electronic word-of-mouth (eWOM). It also aims to investigate the moderating effects of gender and technology self-efficacy for the suggested framework.Design/methodology/approachA sample of 437 participants who had recently booked an online travel package, underwent an analysis using a survey study design. Structural equation modelling with multigroup analysis was used to evaluate the hypotheses and the moderation effect.FindingsThe findings suggest that inefficient market stimulus results in various forms of confusion, further contributing to negative eWOM. The results also imply that technology self-efficacy lessens the effect of various confusions on adverse eWOM, and gender is found to have a moderating effect on the relationships between ineffective marketplace stimuli, confusion and negative eWOM.Practical implicationsThe research offers tourism and hospitality management advice on how to deal with inefficient marketplace stimulation to lessen confusion, which then reduces unfavourable eWOM. Additionally, the moderate impact of technology self-efficacy and gender established through the current study has important ramifications from a tourism managers' perspective.Originality/valueThis study develops and validates an empirical model, which will be utilised as a framework to fully understand consumer confusion brought on by ineffective marketplace stimulation, which causes adverse eWOM. The study also gives new perspectives on the moderating roles of gender and technology self-efficacy, which have received little attention in earlier studies.