bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Seascape Genomics Perspective on Restrictive Genetic Connectivity Overcoming Signals of Local Adaptations in the Green Abalone (Haliotis fulgens) of the California Current System
Authors: Mares‐Mayagoitia Jorge Alberto , Mejía‐Ruíz Paulina , Lafarga‐De la Cruz Fabiola , Micheli Fiorenza , Cruz‐Hernández Pedro , De‐Anda‐Montañez Juan A. , Hyde John , Hernández‐Saavedra Norma Y. , De Jesús‐Bonilla Vladimir S. , Vargas‐Peralta Carmen E. , Flores‐Morales Ana L. , Pares‐Sierra Alejandro F. , Valenzuela‐Quiñonez Fausto 
Published: February 2025

ABSTRACTSeascape genomics facilitates integrative research on eco‐evolutionary forces, such as migration and natural selection, which shape genomic connectivity and structure and provide critical insights for conservation strategies. The green abalone (Haliotis fulgens) is distributed from California, United States, to Baja California Sur, Mexico, and exposed to a latitudinal environmental gradient in the California Current System. This study aimed to investigate genomic population structure and potential local adaptations of green abalone across its distribution. The green abalone exhibits a distinctive neutral genetic structuring influenced by geographic distance and marine currents rather than local adaptations. Analyses using 9100 neutral and 17 outlier SNPs revealed three distinct populations: the North group (California to Ensenada, Baja California), a population on Guadalupe Island, and the South group (coastal locations of the Baja California peninsula). The research underscores the significance of life history traits and larval dispersal in shaping genetic connectivity. Connectivity appears to be influenced by geographic distance on neutral genetic structure, overshadowing natural selection's role. Furthermore, no genome–environment associations to sea surface temperature values were found. Future research should integrate genetic data with ocean circulation modeling to better understand the mechanisms and outcomes of larval dispersal and genetic connectivity. This study emphasizes the importance of both local and binational (USA‐Mexico) conservation efforts, suggesting the development of SNP marker panels for traceability and management. Collaborative strategies could serve as models for binational conservation initiatives in other ecoregions, promoting sustainable management and conservation of green abalone populations and other exploited species across national borders.
Ecohydrological Engineering for the Sustainable Management of Water–Biota Interactions
Authors: Perri Saverio , Levin Simon , Porporato Amilcare 
Published: February 2025

ABSTRACTEcohydrology engineering provides a valuable framework for addressing emerging environmental challenges by integrating ecological and environmental engineering principles. In this study, we discuss the potential of parsimonious, physically based ecohydrological models through the lens of three case studies: sustainable irrigation, urban heat island mitigation via green roofs and mangrove restoration for climate change mitigation. First, we investigate sustainable irrigation strategies, illustrating the trade‐offs between water conservation and soil salinization. This highlights the delicate balance required to optimize crop yield while mitigating soil degradation. Second, we explore the role of green roofs in urban heat island mitigation, showing how vegetation and water dynamics on rooftops can enhance latent heat flux, thereby potentially reducing urban temperatures and improving building energy efficiency. Lastly, we assess the climate mitigation potential of mangrove restoration, accounting for the impacts of salinization and sea‐level rise. We demonstrate that carbon sequestration in mangrove ecosystems may be strongly limited by productivity reduction due to salinity and reduced area availability under sea‐level rise. These case studies illustrate the strengths of simplified ecohydrological models in capturing critical feedbacks and interdependencies between water and biota across diverse environments. By prioritizing adaptive, resilient strategies, EE offers a practical pathway for developing innovative, context‐sensitive solutions that leverage ecosystem dynamics to address pressing environmental issues.
Machine Learning Speeds Up the Discovery of Efficient Porphyrinoid Electrocatalysts for Ammonia Synthesis
Authors: Hu Wenfeng , Song Bingyi , Yang Liming 
Published: February 2025

Two‐dimensional transition metal porphyrinoid materials (2DTMPoidMats), due to their unique electronic structure and tunable metal active sites, have the potential to enhance interactions with nitrogen molecules and promote the protonation process, making them promising electrochemical nitrogen reduction reaction (eNRR) electrocatalysts. Experimentally screening a large number of catalysts for eNRR catalytic performance would consume considerable time and economic resources. First‐principles calculations and machine learning (ML) algorithms could greatly improve the efficiency of catalyst screening. Using this approach, we selected 86 candidates capable of catalyzing eNRR from 1290 types of 2DTMPoidMats, and verified the results with density functional theory (DFT) computations. Analysis of the full reaction pathway shows that MoPp‐meso‐F‐β‐Py, MoPp‐β‐Cl‐meso‐Diyne, MoPp‐meso‐Ethinyl, and WPp‐β‐Pz exhibit the best catalytic performance with the onset potential of −0.22, −0.19, −0.23, and −0.35 V, respectively. This work provides valuable insights into efficient design and screening of eNRR catalysts and promotes the application of ML algorithmic models in the field of catalysis.
Refining Reduced the Number and Content of Compounds of Soybean Oil
Authors: Sun Zhaohui , Jiang Huange , Yang Yiwen , Wang Mengxu , Tong Jingmin , Zhao Lan , Luo Shunian , Wang Maoqing 
Published: February 2025

ABSTRACTTo explore the effect of the refining process on the compounds of soybean oil, three different refined grade soybean oils were detected by ultra performance liquid chromatography‐high‐definition mass spectrometry and chemometrics for finding the differential compounds. The results of PCA and PLS‐DA showed that three different refined grade soybean oils had the clear separation trend and the number and content of compounds in soybean crude oil (SCO) gradually reduced with the increased process of the refining. Our result indicated that the refining processes reduced the number and content of compounds in SCO. The decreased trans fatty acids and other unfavorable quality compounds (free fatty acids) suggested that the refining process was necessary. But, 108 compounds with potential function in SCO were significantly reduced during the process of the refine. Most of these compounds (daidzein, linoleamide, and stearamide, etc.) were reduced during the process of the third‐grade refine and were even not detected after the first‐grade refine. In addition, 16 compounds were not found in SCO and were detected in the refined soybean oil and increased during the refining processes. Our results suggest that refining diminishes the quantity and concentration of compounds with potential function in SCO and should be improved.
Assessment of frailty in patients with heart failure: A new Heart Failure Frailty Score developed by Delphi consensus
Authors: Vitale Cristiana , Berthelot Emmanuelle , Coats Andrew J.S. , Loreena Hill , Albert Nancy M. , Tkaczyszyn Michal , Adamopoulos Stamatis , Anderson Lisa , Anker Markus S. , Anker Stefan D. , Bell Derek , Ben‐Gal Tuvia , Bistola Vasiliki , Bozkurt Biykem , Brooks Poppy , Camafort Miguel , Carrero Juan Jesus , Chioncel Ovidiu , Choi Dong‐Ju , Chung Wook‐Jin , Doehner Wolfram , Fernández‐Bergés Daniel , Ferrari Roberto , Fiuzat Mona , Gomez‐Mesa Juan Esteban , Gustafsson Finn , Jankowska Ewa , Kang Seok‐Min , Kinugawa Koichiro , Khunti Kamlesh , Hobbs F.D. Richard , Lee Christopher , Lopatin Yuri , Maddocks Matthew , Maltese Giuseppe , Marques‐Sule Elena , Matsue Yuya , Miró Òscar , Moura Brenda , Piepoli Massimo , Ponikowski Piotr , Pulignano Giovanni , Rakisheva Amina , Ray Robin , Sciacqua Angela , Seferovic Petar , Sentandreu‐Mañó Trinidad , Sze Shirley , Sinclair Alan , Strömberg Anna , Theou Olga , Tsutsui Hiroyuki , Uchmanowicz Izabella , Vidan Maria Teresa , Volterrani Maurizio , von Haehling Stephan , Yoo Byungsu , Zhang Jian , Zhang Yuhui , Metra Marco , Rosano Giuseppe Massimo Claudio 
Published: February 2025

AbstractAimsThe Heart Failure Frailty Score (HFFS) is a novel, multidimensional tool to assess frailty in patients with heart failure (HF). It has been developed to overcome limitations of existing frailty assessment tools while being practical for clinical use. The HFFS reflects the concept of frailty as a multidimensional, dynamic and potentially reversible state, which increases vulnerability to stressors and risk of poor outcomes in patients with HF.Methods and resultsThe HFFS was developed through a Delphi consensus process involving 54 international experts. This approach involved iterative rounds of questionnaires and interviews, where a panel of experts provided their opinions on specific questions prepared by the Steering Committee. The experts were invited to vote and share their views anonymously, using a 5‐point Likert scale over iterative rounds. An 80% threshold was set for agreement or disagreement for each statement. Twenty‐two variables from four domains (clinical, functional, psycho‐cognitive and social) have been selected for inclusion in the HFFS after the third round of the Delphi process. A shorter version (S‐HFFS), including 10 variables, has also been developed for daily clinical use.ConclusionsThe HFFS is a new multidimensional tool for the identification of frailty in patients with HF. It should also enables healthcare providers to identify potential ‘red flags’ for frailty in order to develop personalized care plans. The next step will be to validate the new score in patients with HF.
NHC‐Free Ketone Synthesis via Radical Coupling Mechanisms
Authors: Wang Lifeng , Cai Yuxing , Huang Yong 
Published: February 2025

AbstractKetone synthesis via radical coupling mechanisms has recently gained significant attention. Traditionally, these methods have relied on N‐heterocyclic carbenes (NHCs) to generate the key ketyl radical intermediate, either through single‐electron reduction of acyl azolium or single‐electron oxidation involving the Breslow intermediate. In this report, we introduce a novel photoredox protocol that directly generates ketyl radicals from stable acyl imidazoles without the need for NHCs. This approach not only simplifies the process, but also demonstrates broad tolerance for various alkyl radical substrates. Additionally, it can be extended to facilitate relay‐radical coupling reactions.
Exogenous ATP Functions in Alleviating the Decrease in Quality of Grape (Vitis vinifera L.) Fruits After Harvest
Authors: Deng Yaxin , Zhao Mengjie , Jia Lingyun , Liang Junyu , Wang Fei , Yao Meiling , Mitina Irina , Zhang Ji , Feng Hanqing , Arpentin Gheorghe 
Published: February 2025

ABSTRACTOur current study investigated the effects of exogenous ATP (adenosine triphosphate) on the post‐harvest quality of grape (Vitis vinifera L.) fruits. The results demonstrated that treatment with exogenous ATP effectively alleviated the decreases in fruit firmness, total soluble solids (TSS), vitamin C content and soluble protein of the harvested grape fruits. Furthermore, exogenous ATP also significantly enhanced the content of the titratable acidity (TA) of grape fruits after harvest. This was reflected by the observations that ATP treatment increased the hardness, TSS content, vitamin C content and soluble protein content of the harvested grape fruits by 14.39%, 6.29%, 31.05% and 15.18% on average during 8 days of storage, respectively, compared to the controls. In addition, the harvested grape fruits treated with exogenous ATP had higher activities of SOD (superoxide dismutase) and CAT (catalase) and higher contents of total phenolics and flavonoids compared to the controls. This was reflected by the observations that ATP treatment increased the SOD activity, CAT activity, flavonoid content and total phenol content by 39.79%, 19.43%, 17.74% and 15.06% on average during 8 days of storage, respectively, compared to the controls. These observations highlighted that exogenous ATP can function in alleviating the decrease in quality of grape fruits after harvest and increasing the antioxidative ability of harvested grape fruits during the storage period. Consequently, treatment with exogenous ATP could offer an efficacious approach for preserving the quality of grape fruits during the storage period.
Evaluation of serum and peritoneal fluid mannose‐binding lectin associated serine protease‐3, adipsin, properdin, and complement factor‐H levels in endometriosis patients
Authors: Eşkin Tanrıverdi Merve Didem , Kaya Sezginer Ecem , Erol Koç Esin Merve , Moraloğlu Tekin Özlem 
Published: February 2025

AbstractObjectiveEndometriosis is a chronic disease which has been reported to be associated with distorted immune mechanisms. The alternative pathway is a complement system which plays a role in immune defense. The present study aimed to evaluate whether the level of alternative complement molecules differ in women with endometriosis compared to heathy individuals.MethodsA total of 58 women participated in this prospective research. Women with a diagnosis of endometriosis confirmed by laparoscopy (n = 32) were compared to healthy women (n = 26) in terms of serum adipsin, properdin, mannose‐binding lectin‐associated serine protease‐3 and complement factor‐H (CFH) levels. The peritoneal fluid samples which were taken during the endometriosis surgery were also analyzed in terms of the complement levels. The clinical and demographic data including the serum CA‐125 level and pelvic pain were also analyzed. SPSS version 23.0 was used in statistical analysis.ResultsThe serum levels of adipsin and CFH were found to be significantly increased in women with endometriosis (P = 0.027 and P = 0.040, respectively). Serum adipsin level was found to significantly correlate with serum CA‐125 level (r = 0.320, P = 0.015), serum CFH level (r = 0.705, P < 0.001), and degree of the pelvic pain complaint (r = 0.326, P = 0.013). A strong, positive correlation was also observed between peritoneal fluid levels of adipsin, and CFH (r = 0.593; P < 0.001).ConclusionTo the best of our knowledge, the present study is the first to evaluate the alternative complement system in women with endometriosis. The current findings may be noteworthy to elucidate the possible role of the key molecules of the alternative pathway in endometriosis pathogenesis.
Possible new mechanisms of primary drug resistance in NSCLC with EGFR mutation treated with Osimertinib
Authors: Shao Lujing , Li Tong , Jia Xinyan , Zhang Xinyu , Li Qi , Dong Chunyan 
Published: February 2025

AbstractIn this study, a patient with lung adenocarcinoma harboring an EGFR mutation exhibited primary resistance to the targeted EGFR inhibitor Osimertinib after 2 months of treatment. As the disease advanced, further genetic analysis revealed the emergence of additional mutations in ARID1A, NTRK1, and ZRSR2, alongside the existing EGFR mutation. Subsequent treatment with Pemetrexed resulted in a significant reduction in liver metastases. Protein mass spectrometry sequencing and immunohistochemical analysis collectively indicated that the PI3K/mTOR pathway mediates the mechanism through which these gene mutations confer primary drug resistance. Evidence demonstrates that the co‐occurrence of EGFR and ARID1A mutations diminishes the efficacy of EGFR tyrosine kinase inhibitors (EGFR TKIs). Consequently, it is hypothesized that mutations in NTRK1 and ZRSR2, which are implicated in the PI3K/mTOR pathway, contribute to the primary resistance observed with Osimertinib treatment. In this case, the illness was effectively managed through prompt adjustments to the treatment regimen and the rapid administration of chemotherapy drugs. This finding also constitutes the first evidence that mutations in NTRK1 and ZRSR2 are pivotal in the development of primary resistance to Osimertinib. Consequently, it is imperative to conduct genetic testing at the earliest opportunity and modify the treatment plan accordingly.
Approximating Fixed‐Horizon Forecasts Using Fixed‐Event Forecasts
Authors: Knüppel Malte , Vladu Andreea L. 
Published: February 2025

ABSTRACTMany forecast surveys ask their participants for fixed‐event forecasts. As fixed‐event forecasts have seasonal properties, users often employ an ad hoc approach to approximate fixed‐horizon forecasts based on these fixed‐event forecasts. We derive an optimal approximation for fixed‐horizon forecasts by minimizing the mean‐squared approximation error. Like the ad hoc approach, our approximation employs a weighted average of the fixed‐event forecasts. The optimal weights tend to differ substantially from those of the ad hoc approach. In empirical applications, the gains from using optimal instead of ad hoc weights turn out to be sizeable. The approximation approach proposed can also be useful in other applications.
Physicochemical Characterization of Hyaluronic Acid‐Methylcellulose Semi‐Gels for Mitochondria Transplantation
Authors: Ahmed A. Jamie , Gallegos Zoe A. , Dinar Md Abu Monsur , Sullivan Patrick G. , DeRouchey Jason E. , Patel Samir P. , Rabchevsky Alexander G. , Dziubla Thomas D. 
Published: February 2025

ABSTRACTTraumatic spinal cord injury (SCI) presents a significant medical challenge due to its intricate nature and treatment complexities. SCI can cause physical impairments by affecting neural and motor functions as well as initiating a series of pathophysiological events exacerbating the initial trauma. Leakage from ruptured neurons and vessels disrupt ionic balance and induces excitotoxicity, leading to progressive cellular degeneration. Introducing mitochondria to the SCI lesion has shown potential in attenuating secondary injury. Mitochondria transplantation improves cellular bioenergetics and reduces concentration of reactive oxygen species achieving homeostasis and neuroprotection. Nonetheless, keeping mitochondria viable outside cell environment for a time longer than a few minutes proves to be challenging. Additionally, localized delivery to the injury site has also been limited by other factors including flow rate of cerebrospinal fluid that washes away mobilized organelle from the compromised tissue site. Previously we showed that using hyaluronic acid‐methylcellulose semi‐gels (HAMC) as a biocompatible, erodible thermogelling delivery vehicle helped to overcome some of these challenges. HAMC allows for controlled release at and around the injury site, utilizing the reverse thermogelling property of MC. Sustained release of mitochondria at slower rate can increase their uptake in spinal tissue. To better optimize the semi‐gel delivery of mitochondria requires a more complete understanding of the physicochemical properties of the HAMC semi‐gels. We have used ultraviolet–visible spectroscopy to measure optical density of HAMC semi‐gels for different HA to MC ratios and examine the temperature dependent gelation properties above their low critical solution temperature (LCST). The viscosity and degree of crystallinity of the resulting HAMC semi‐gels were also assessed. Semi‐gel erosion and mitochondrial release over time were studied using a fluorescence microplate reader. Lastly, seahorse assay was used to study released mitochondria respiration and viability after incubation in HAMC semi‐gel.
Hypoxic Secretome and Exosomes Derived From Human Glioblastoma Cells (U87MG) Promote Protumorigenic Phenotype of Microglia in Vitro
Authors: Pancholi Sangati , Shah Ritvi , Bose Utsav , Yadav Ankit , Murukan Karthik , Pillai Prakash 
Published: February 2025

ABSTRACTGlioblastoma multiforme (GBM), a highly heterogeneous CNS tumor known for its highest incidence rates and poor prognosis has shown limited success in the therapies due to hypoxia—driving immune‐suppression in the tumor microenvironment (TME). Emerging evidence highlights the involvement of tumor cell‐derived exosomes in tumor‐associated microglia polarization via transfer of exosomal onco‐proteins and miRNAs. Although the regulatory role of long noncoding RNAs (lncRNAs) in immune signaling are known, its mechanism in microglial polarization via exosomes in GBM still remains poorly understood. In our study, we found that in comparison to the normoxic GBM‐derived exosomes lncRNA H19 was significantly upregulated in hypoxic GBM‐derived exosomes. Hypoxic GBM‐derived exosomes and secretome (conditioned media) caused the reduction in the % phagocytosis of microglia as compared with the control group. Moreover, GBM secretome caused increase in the M2‐specific genes (IL10, STAT‐3, CD163, CD206) in microglia indicating its polarization to the protumorigenic (M2) phenotype. LncRNA H19 knocked down GBM‐secretome treatment in microglia further reduced the STAT‐3 expression indicating H19 mediated signaling. Overall, our results suggest the involvement of hypoxic exosomes and lncRNA H19 in microglial polarization and H19 as a potential target.
Characteristics of the parent‐child relationship at 18‐months of age predict the onset and type of mental disorders in childhood and adolescence: A longitudinal nested birth cohort study
Authors: Nilsson Kristine Kahr , Grønnebæk Lasse , Østergård Ole Karkov , Landorph Susanne , Houmann Tine , Olsen Else Marie , Skovgaard Anne Mette 
Published: February 2025

AbstractBackgroundThe quality of the parent‐child relationship in the first years of childhood has been associated with various long‐term mental health outcomes. However, it is unclear whether specific characteristics of the early parent‐child relationship predict the onset of mental disorders. The aim of this study was therefore to examine child, parent, and dyadic characteristics of the early parent‐child relationship as predictors of mental disorders in childhood and adolescence.MethodAs a part of the Copenhagen Child Cohort 2000, a child cohort of 295 children participated in the study at 18‐months of age and were subsequently followed until late adolescence. Child, parent, and dyadic dimensions of the early parent‐child relationship were assessed using the observational Parent‐Child Early Relational Assessment. The outcomes were mental disorders subsequently diagnosed in childhood and adolescence.ResultsCox‐regression analyses suggested that parent characteristics involving negative affect and behavior, intrusiveness and insensitivity during early parent child interactions were associated with increased risk of the child being diagnosed with a mental disorder at later ages. However, variation was noted across specific diagnoses. Parental negative affect and behavior was specifically associated with increased risk of attention deficit hyperactivity disorder, while infant dysregulation and irritability was specifically associated with increased risk of autism spectrum disorders. These results remained significant after adjusting for parental mental disorders and early mental health problems of the child.ConclusionDisorder‐specific associations were observed between dysfunctions in the early parent‐child relationship and subsequent mental disorders. Improving such early relational dysfunctions may support the goals of early interventions that aim to mitigate the risk of mental disorders from a very early age.