bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ginsenoside-Rd attenuates oxidative damage related to aging in senescence-accelerated mice
Authors: Yokozawa Takako , Satoh Akiko , Cho Eun Ju 
Published: January 2004

Abstract
Among the various theories of the aging process, the free radical theory, which proposes that deleterious actions of free radicals are responsible for the functional deterioration associated with aging, has received widespread attention. The theory suggests that enhancement of the antioxidative defence system to attenuate free-radical-induced damage will counteract the aging process. We used senescence-accelerated mice (SAM) to investigate the relationship between aging and the antioxidative defence system and evaluated the effects of ginsenoside-Rd, the saponin from ginseng, by measuring antioxidative defence system parameters, including the glutathione (GSH)/glutathione disulfide (GSSG) redox status, antioxidative enzyme activity and level of lipid peroxidation. SAM at 11 months of age (old SAM) showed a significantly lower hepatic GSH/GSSG ratio, due to decreased GSH and increased GSSG levels, than SAM at 5 weeks of age (young SAM). However, the administration of ginsenoside-Rd at a dose of 1 or 5 mg kg−1 daily for 30 days to 10-month-old SAM significantly increased GSH, but decreased GSSG, resulting in elevation of the GSH/GSSG ratio. In addition, ginsenoside-Rd increased the activity of glutathione peroxidase (GSH-Px) and glutathione reductase that were both significantly lower in old SAM than in young SAM. This suggests that ginsenoside-Rd could play a crucial role in enhancing the defence system through regulation of the GSH/GSSG redox status. Moreover, decreases in the superoxide dismutase (SOD) and catalase activity in old SAM compared with young SAM were also revealed, indicating that the aging process resulted in suppression of the antioxidative defence system. However, ginsenoside-Rd did not affect SOD and catalase activity. As catalase is localized in peroxisome granules and GSH-Px is present in the cytoplasm and mitochondrial matrix, the site of ginsenoside-Rd action may be the cytoplasm and mitochondrial matrix. Furthermore, the serum and liver malondialdehyde levels, indicators of lipid peroxidation, were elevated with aging, while ginsenoside-Rd inhibited lipid peroxidation. This study indicates that the aging process leads to suppression of the antioxidative defence system and accumulation of lipid peroxidation products, while ginsenoside-Rd attenuates the oxidative damage, which may be responsible for the intervention of GSH/GSSG redox status.
Antihypertensive and anti-arrhythmic effects of an extract of Radix Stephaniae Tetrandrae in the rat
Authors: Yu X-C , Wu S , Chen C-F , Pang K T , Wong T M 
Published: January 2004

Abstract
In this study, we determined the effects of an extract of Radix Stephaniae Tetrandrae (RST) on arterial blood pressure and heart weight in deoxycorticosterone acetate-salt (DOCA-salt) hypertensive rats. We also determined the effects of the extract on arrhythmia and infarct induced by myocardial ischaemia and reperfusion in anaesthetized rats. We further compared the effects of the extract with those of tetrandrine, which makes up 7% of the extract and is known to act as a calcium-channel antagonist, and verapamil, a prototype calcium-channel antagonist. Treatment with RST extract returned the arterial blood pressure, cardiac compliance and coronary flow towards normal, and reduced right ventricular hypertrophy in the DOCA-salt hypertensive rat. In the anaesthetized rat, the RST extract reduced arrhythmia and infarct size induced by myocardial ischaemia and reperfusion; the effects were similar to those of tetrandrine and verapamil. The findings indicate that the RST extract acts like a calcium-channel antagonist. It may be used in the treatment of cardiovascular diseases, as are the calcium-channel antagonist and tetrandrine. More interestingly, the effects of the RST extract were of the same potency as tetrandrine. Since only 7% of the extract was tetrandrine, the observation indicates that tetrandrine was not the only component that was responsible for the actions of the extract.
Water extract isolated from Chelidonium majus enhances nitric oxide and tumour necrosis factor-α production via nuclear factor-κB activation in mouse peritoneal macrophages
Authors: Chung Hwan-Suck , An Hyo-Jin , Jeong Hyun-Ja , Won Jin-Hee , Hong Seung-Heon , Kim Hyung-Min 
Published: January 2004

Abstract
Chelidonium majus is used to treat several inflammatory diseases and tumours. We have examined the effect of C. majus on nitric oxide (NO) production using mouse peritoneal macrophages. When C. majus was used in combination with recombinant interferon-γ (rIFN-γ, 10U mL−1), there was a marked cooperative induction of NO production. Treatment of rIFN-γ plus C. majus (1 mg mL−1) in macrophages caused a significant increase in tumour necrosis factor-α (TNF-α) production. The increased production of NO and TNF-α from rIFN-γ plus C. majus-stimulated cells was almost completely inhibited by nuclear factor-κB (NF-κB) inhibitor, pyrrolidine dithiocarbamate (100 μM). These findings demonstrated that C. majus increased the production of NO and TNF-α by rIFN-γ-primed macrophages and suggested that NF-κB played a critical role in mediating the effects of C. majus.
Acute and topic anti-edematogenic fractions isolated from the seeds of Pterodon pubescens
Authors: Silva M C C , Gayer C R M , Lopes C S , Calixto N O , Reis P A , Passaes C P B , Paes M C , Dalmau S R , Sabino K C C , Todeschini A R , Coelho M G P 
Published: January 2004

Abstract
We previously demonstrated that alcoholic extracts from Pterodon pubescens Benth. (Sucupira branca, Leguminosae) seeds exhibit anti-arthritic activity. In the present work we show that the oleaginous extract obtained from P. pubescens seeds (OEP) exhibits acute or topic anti-edematogenic activity when tested in carrageenan-induced paw edema or in croton oil-induced ear edema assays, respectively. Four fractions were obtained from OEP by sequential liquid–liquid extraction. The anti-edematogenic properties were predominant in the hexanic fraction, which was further fractionated by HPLC, yielding three sub-fractions (PF1.1, PF1.2 and PF1.3). PF1.1 and PF1.3 showed potent acute and topic anti-edematogenic activity. The PF1.2 sub-fraction, although not active in the carrageenan assay, exhibited a potent anti-edematogenic activity in the croton oil-induced ear edema. This sub-fraction shows a maximum efficacy similar to indometacin in a lower dose. The PF1.1 sub-fraction presented a complex mixture containing furane diterpene derivatives of vouacapan. PF1.2 consists of a single substance, geranylgeraniol, as determined by GC/MS and NMR, while PF1.3 contains farnesol.
Disposition of amphotericin B in the isolated perfused rat liver
Authors: Hong Ying , Ramzan Iqbal , McLachlan Andrew J 
Published: January 2004

Abstract
The hepatic disposition and biliary excretion of amphotericin B were investigated in the isolated perfused rat liver (IPRL). Bolus dose of 50 μg, 99 μg and 198 μg amphotericin B in lipoprotein-free perfusate and 198 μg amphotericin B in perfusate with 1 μM high-density lipoprotein (HDL) or 1 μM low-density lipoprotein (LDL) were examined in the IPRL. Amphotericin B concentration in perfusate was measured using a validated HPLC assay. Amphotericin B was eliminated from the perfusate in a biexponential manner. The hepatic clearance (CLH) increased in proportion to the dose administered (0.27±0.05 mL min−1 at low dose, 0.54±0.23 mL min−1 at medium dose and 1.06±0.24 mL min−1 at high dose), indicating non-linear hepatic disposition of amphotericin B. The hepatic extraction ratio of amphotericin B was very low (0.066±0.015). Tissue-to-perfusion partition coefficient, calculated at 120 min, increased 1.5 fold from 9.8±1.7 at low dose to 15.9±6.4 at high dose, suggesting the significant uptake and extensive retention of amphotericin B in the liver. Biliary excretion made only minor contribution to amphotericin B elimination in the IPRL, representing around 1–3% of the dose administered. No metabolites were detected in perfusate, bile and liver samples. The hepatic disposition of amphotericin B was not affected by the presence of HDL and LDL in the perfusate. In conclusion, the hepatic disposition of amphotericin B demonstrates restrictive elimination and is concentration-dependent, consistent with carrier-mediated uptake, and lipoproteins do not influence amphotericin B hepatobiliary disposition.
Predicting the precipitation of poorly soluble weak bases upon entry in the small intestine
Authors: Kostewicz Edmund S , Wunderlich Martin , Brauns Ulrich , Becker Robert , Bock Thomas , Dressman Jennifer B 
Published: January 2004

Abstract
Solubility and dissolution relationships in the gastrointestinal tract can be critical for the oral bioavailability of poorly soluble drugs. In the case of poorly soluble weak bases, the possibility of drug precipitation upon entry into the small intestine may also affect the amount of drug available for uptake through the intestinal mucosa. To simulate the transfer out of the stomach into the intestine, a transfer model was devised, in which a solution of the drug in simulated gastric fluid is continuously pumped into a simulated intestinal fluid, and drug precipitation in the acceptor medium is examined via concentration–time measurements. The in-vitro precipitation of three poorly soluble weakly basic drugs, dipyridamole, BIBU 104 XX and BIMT 17 BS, was investigated. For all three, extensive supersaturation was achieved in the acceptor medium. Under simulated fasted-state conditions, precipitation occurred for all three compounds whereas under simulated fed-state conditions, the higher concentrations of bile components and the lower pH value in the acceptor medium inhibited precipitation at concentrations corresponding to usual doses in all cases. Comparison with pharmacokinetic data indicated that a combination of transfer model data with solubility and dissolution profiles should lead to better predictions of in-vivo behaviour of poorly soluble weak bases.
Evaluation of the impact of altered lipoprotein binding conditions on halofantrine induced QTc interval prolongation in an anaesthetized rabbit model
Authors: McIntosh Michelle P , Batey Andrew J , Coker Susan J , Porter Christopher J H , Charman William N 
Published: January 2004

Abstract
Halofantrine has been observed to cause QT interval prolongation in susceptible patients and the effect has most commonly been observed after post-prandial administration. Halofantrine-induced QT prolongation occurs in conjunction with a significant increase in plasma halofantrine concentrations and an increase in halofantrine association with post-prandial plasma lipoproteins. The increased association of halofantrine with post-prandial lipoproteins is accompanied by a marked change in drug distribution between the different plasma lipoprotein fractions. This study was designed to evaluate the putative role of myocardium-based lipoprotein receptor-mediated uptake of lipoproteins as a possible contributing factor to the observed effect of halofantrine on QT intervals. The extent of QT interval prolongation following intravenous halofantrine administration (10 mg kg−1) to normolipidaemic (fasted) or hyperlipidaemic (induced with Intralipid infusion) anaesthetized New Zealand White rabbits (n = 6) was determined, as was the distribution of halofantrine between the plasma lipoprotein classes. The results, however, were in contrast to the suggested hypothesis since the QT interval was reduced (and not increased) after halofantrine administration to hyperlipidaemic rabbits relative to fasted rabbits. Therefore, it is unlikely that lipoprotein-based uptake of halofantrine into the myocardium is a major contributor to the previously observed increase in QT prolongation after post-prandial administration of halofantrine.
Is nose-to-brain transport of drugs in man a reality?
Authors: Illum Lisbeth 
Published: January 2004

Abstract
The blood–brain barrier that segregates the brain interstitial fluid from the circulating blood provides an efficient barrier for the diffusion of most, especially polar, drugs from the blood to receptors in the central nervous system (CNS). Hence limitations are evident in the treatment of CNS diseases, such as Parkinson's and Alzheimer's diseases, especially exploiting neuropeptides and similar polar and large molecular weight drugs. In recent years interest has been expressed in the use of the nasal route for delivery of drugs to the brain, exploiting the olfactory pathway. A wealth of studies has reported proof of nose-to-brain delivery of a range of different drugs in animal models, such as the rat. Studies in man have mostly compared the pharmacological effects (e.g. brain functions) of nasally applied drugs with parenterally applied drugs and have shown a distinct indication of direct nose-to-brain transport. Recent studies in volunteers involving cerebrospinal fluid sampling, blood sampling and pharmacokinetic analysis after nasal, and in some instances parenteral administration of different drugs, have in my opinion confirmed the likely existence of a direct pathway from nose to brain.
Cochlear implantation in chronic otitis media
Authors: Incesulu A. , Kocaturk S. , Vural M. 
Published: January 2004

Patients with chronic otitis media (COM) may have profound sensorineural hearing loss either due to the disease process or secondary to a surgical procedure. Some patients who are candidates for cochlear implantation may have COM coincidentally. The patients in this group need special attention when cochlear implantation is applied. The aim of this study is to evaluate the potential risks and complications in patients with COM.Cochlear implantation was performed in six patients with COM or an infected radical cavity and profound hearing loss. Five of them underwent a two-stage operation, and one had a single-stage operation. Cochlear implantation was performed in all patients without complications. The follow-up period was uneventful.Although such patients have some potential risks, when certain surgical rules are followed very strictly cochlear implantation can be successfully performed in patients with COM.
Third and fourth branchial pouch anomalies
Authors: Rea P. A. , Hartley B. E. J. , Bailey C. M. 
Published: January 2004

Congenital sinuses arising from the pyriform fossae are rare. They may present as neck cysts, recurrent neck abscesses, thyroiditis or neonatal stridor. Children typically undergo multiple surgical procedures
before the diagnosis is made.We present our recent experience of five third-pouch sinuses and four fourth-pouch sinuses, including one bilateral case. The median age at presentation was four years. The mean number of operations prior to diagnosis was six (range 0-13), with a mean delay to diagnosis of six years. The diagnosis was made by barium swallow, by ultrasound, at pharyngoscopy or at open surgery. The median age at definitive surgery was 10 years. Definitive treatment involved complete excision of the sinus and neck mass in seven cases. Two sinuses were treated with endoscopicdiathermy to their internal opening.Adoption of the correct nomenclature, diagnostic tools and operative requirements for these cases will help reduce the significant morbidity.
Acid reflux management: ENT perspective
Authors: Ahmad Ijaz , Batch A. J. G. 
Published: January 2004

Otolaryngological manifestations of acid reflux include a wide range of pharyngeal and laryngeal symptoms; and the constellation of symptoms has been called laryngopharyngeal reflux.This is a prospective study in a cohort of patients with various throat symptoms suggestive of laryngopharyngeal reflux (LPR) who underwent flexible oesophago-gastroscopy, as a principal investigation. The aims were to look at the most reliable symptom(s) and sign(s), the diagnostic role of flexible oesophago-gastroscopy and the treatment response in these patients.The endoscopy score of 0 to 3 was based on endoscopic findings and the treatment response was measured from 0 to 100 per cent improvement of symptoms, as described by the patients.There were a total of 303 patients, 174 females and 129 males with ages ranging from 19 to 88 years. Seventy-five per cent had had symptoms for more than a year. Fifteen per cent were smokers. Globus, voice change, sore throat, dysphagia and cough were the predominant symptoms. Most patients, however, presented with a complex of various other secondary symptoms.The endoscopic findings were abnormal in 98 per cent of patients. Apart from the finding of non-specific hyperaemia, usually of the posterior larynx (13 per cent), lesions of the larynx and vocal folds were surprisingly uncommon.Proton pump inhibitors (PPI) were prescribed in 90 per cent of patients. A total of 233 (76.8 per cent) responded to treatment. The improvement of symptoms ranged from 25 per cent in 36 (23 per cent), 50 per cent in 60 (20 per cent), 75 per cent in 59 (19 per cent) and 100 per centin 78 (26 per cent) patients.Accumulative analysis of variance showed a significant difference between treatment responders and non-responders (p <0.04). In a logistic regression model patients with globus, voice change and gastric prolapse were more likely to respond to treatment (p <0.04).It can be concluded that voice change, sore throat, globus and cough choking are the most reliable symptoms of laryngopharyngeal reflux. Voice change and globus symptoms can be of predictive value in terms of successful treatment response. Flexible oesophago-gastroscopy (FOG) isa simple, safe and reliable way of assessment in these cases and treatment with PPI can be effective in the majority of patients.
Carbon‐Nanotube‐Reinforced Zr‐Based Bulk Metallic Glass Composites and Their Properties
Authors: Bian Z. , Wang R. J. , Wang W. H. , Zhang T. , Inoue A. 
Published: January 2004

AbstractIn this paper, we systematically report the preparation of carbon‐nanotube (CNT)‐reinforced Zr‐based bulk metallic glass (BMG) composites. The physical and mechanical properties of the composites were investigated. Compressive testing shows that the composites still display high fracture strength. Investigation also shows that the composites have strong ultrasonic attenuation characteristics and excellent wave absorption ability. The strong wave absorption implies that CNT‐reinforced Zr‐based BMG composites, besides their excellent mechanical properties, may also have significant potential for applications in shielding acoustic sound or environmental noise.
Microtubule‐Templated Biomimetic Mineralization of Lepidocrocite
Authors: Boal A. K. , Headley T. J. , Tissot R. G. , Bunker B. C. 
Published: January 2004

AbstractProtein microtubules (MTs) 25 nm in diameter and tens of micrometers long have been used as templates for the biomimetic mineralization of FeOOH. Exposure of MTs to anaerobic aqueous solutions of Fe2+ buffered to neutral pH followed by aerial oxidation leads to the formation of iron oxide coated MTs. The iron oxide layer was found to grow via a two‐step process: initially formed 10–30 nm thick coatings were found to be amorphous in structure and comprised of several iron‐containing species. Further growth resulted in MTs coated with highly crystalline layers of lepidocrocite with a controllable thickness of up to 125 nm. On the micrometer size scale, these coated MTs were observed to form large, irregular bundles containing hundreds of individually coated MTs. Iron oxide grew selectively on the MT surface, a result of the highly charged MT surface that provided an interface favorable for iron oxide nucleation. This result illustrates that MTs can be used as scaffolds for the in‐situ production of high‐aspect‐ratio inorganic nanowires.
Designed Self‐Assembled β‐Sheet Peptide Fibrils as Templates for Silica Nanotubes
Authors: Meegan J. E. , Aggeli A. , Boden N. , Brydson R. , Brown A. P. , Carrick L. , Brough A. R. , Hussain A. , Ansell R. J. 
Published: January 2004

AbstractSol–gel condensation of tetraethoxysilane in the presence of designed self‐assembled β‐sheet peptide fibril templates, followed by template extraction, yields hollow silica nanotubes. The nanotubes are hundreds of nanometers long and possess a central pore of ∼ 3.5 nm, determined by the fibril template diameter. The effects of synthesis conditions have been investigated and the resultant silica materials characterized by various techniques.Silica nanostructures with various morphologies have been produced previously using supramolecular organic assemblies as templates. Hollow nano‐ or microtubes, which may have applications in separations, catalysis, nano‐optics, and ‐electronics have been of particular interest. Peptide‐based templates are especially interesting because of their relevance to biological silica microstructure formation. The new fibrillar peptide templates described here have the advantages of prescribed diameter, twist pitch, and handedness, which should impart chirality on the resulting silica nanotubes, providing control of the internal surface architecture by appropriate peptide design.
Optimization of a Quasi‐Solid‐State Dye‐Sensitized Photoelectrochemical Solar Cell Employing a Ureasil/Sulfolane Gel Electrolyte
Authors: Stathatos E. , Lianos P. , Surca Vuk A. , Orel B. 
Published: January 2004

AbstractA quasi‐solid‐state, dye‐sensitized photoelectrochemical solar cell employing a gel electrolyte obtained by sol–gel chemistry is described. The gel electrolyte is based on a ureasil precursor (i.e., a poly(propylene oxide) oligomer end‐capped by triethoxysilane groups through urea bridges) and sulfolane and it incorporates the I3–/I– redox couple. It is shown that the combination of these two reagents prevents crystallization of KI, thus ensuring a long life for the cell and a satisfactory overall efficiency that surpasses 5 %. Cell efficiency increases with temperature. Optimization of gel‐electrolyte performance has been obtained by studying mobility with fluorescence‐quenching techniques complemented by direct‐current conductivity measurements.
Peptide Templates for Nanoparticle Synthesis Derived from Polymerase Chain Reaction‐Driven Phage Display
Authors: Naik R. R. , Jones S. E. , Murray C. J. , McAuliffe J. C. , Vaia R. A. , Stone M. O. 
Published: January 2004

AbstractPhage peptide display libraries are commonly used to select peptides that bind to inorganic surfaces (metals, metal oxides, and semiconductors). These binding peptides can serve as templates to control the nucleation and growth of inorganic nanoparticles in vitro. In this report, we describe the identification of a unique set of sequences that bind to silver and cobalt nanoparticles from a phage peptide display library using a polymerase chain reaction (PCR)‐driven method. The amino acid sequences obtained by the PCR method are a distinct set of sequences that would otherwise be missed using the regular panning method. Peptides identified by the method described here are also shown to function as templates for the synthesis of silver and cobalt platinum nanoparticles.