bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conflict-free joint decision by lag and zero-lag synchronization in laser network
Authors: Ito Hisako , Mihana Takatomo , Horisaki Ryoichi , Naruse Makoto 
Published: February 2024

AbstractWith the end of Moore’s Law and the increasing demand for computing, photonic accelerators are garnering considerable attention. This is due to the physical characteristics of light, such as high bandwidth and multiplicity, and the various synchronization phenomena that emerge in the realm of laser physics. These factors come into play as computer performance approaches its limits. In this study, we explore the application of a laser network, acting as a photonic accelerator, to the competitive multi-armed bandit problem. In this context, conflict avoidance is key to maximizing environmental rewards. We experimentally demonstrate cooperative decision-making using zero-lag and lag synchronization within a network of four semiconductor lasers. Lag synchronization of chaos realizes effective decision-making and zero-lag synchronization is responsible for the realization of the collision avoidance function. We experimentally verified a low collision rate and high reward in a fundamental 2-player, 2-slot scenario, and showed the scalability of this system. This system architecture opens up new possibilities for intelligent functionalities in laser dynamics.
Probing delivery of a lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging
Authors: Bera Kajari , Rojas-Gómez Renán A. , Mukherjee Prabuddha , Snyder Corey E. , Aksamitiene Edita , Alex Aneesh , Spillman Darold R. , Marjanovic Marina , Shabana Ahmed , Johnson Russell , Hood Steve R. , Boppart Stephen A. 
Published: February 2024

AbstractThe COVID-19 pandemic triggered the resurgence of synthetic RNA vaccine platforms allowing rapid, scalable, low-cost manufacturing, and safe administration of therapeutic vaccines. Self-amplifying mRNA (SAM), which self-replicates upon delivery into the cellular cytoplasm, leads to a strong and sustained immune response. Such mRNAs are encapsulated within lipid nanoparticles (LNPs) that act as a vehicle for delivery to the cell cytoplasm. A better understanding of LNP-mediated SAM uptake and release mechanisms in different types of cells is critical for designing effective vaccines. Here, we investigated the cellular uptake of a SAM-LNP formulation and subsequent intracellular expression of SAM in baby hamster kidney (BHK-21) cells using hyperspectral coherent anti-Stokes Raman scattering (HS-CARS) microscopy and multiphoton-excited fluorescence lifetime imaging microscopy (FLIM). Cell classification pipelines based on HS-CARS and FLIM features were developed to obtain insights on spectral and metabolic changes associated with SAM-LNPs uptake. We observed elevated lipid intensities with the HS-CARS modality in cells treated with LNPs versus PBS-treated cells, and simultaneous fluorescence images revealed SAM expression inside BHK-21 cell nuclei and cytoplasm within 5 h of treatment. In a separate experiment, we observed a strong correlation between the SAM expression and mean fluorescence lifetime of the bound NAD(P)H population. This work demonstrates the ability and significance of multimodal optical imaging techniques to assess the cellular uptake of SAM-LNPs and the subsequent changes occurring in the cellular microenvironment following the vaccine expression.
In ovo feeding of methionine affects antioxidant status and growth-related gene expression of TETRA SL and Hungarian indigenous chicks
Authors: Lugata James K. , Ndunguru Sawadi F. , Reda Gebrehaweria K. , Gulyás Gabriella , Knop Renáta , Oláh János , Czeglédi Levente , Szabó Csaba 
Published: February 2024

AbstractMethionine (Met) plays a substantial role in poultry due to its involvement in several pathways, including enhancing antioxidant status and improving growth performance and health status. This study examined how in ovo feeding of Met affects hatching performance, antioxidant status, and hepatic gene expression related to growth and immunity in the TETRA-SL LL hybrid (TSL) commercial layer and Hungarian partridge colored hen (HPC) indigenous genotypes. The eggs were injected with saline, DL-Met, and L-Met on 17.5 days of embryonic development. The results showed that the in ovo feeding of DL-Met significantly increased the hatching weight and ferric reducing the ability of the plasma (FRAP) compared with L-Met. The in ovo feeding of either Met source enhanced the liver health and function and hepatic antioxidant status of the chicks. The genotype’s differences were significant; the TSL genotype had better hatching weight, an antioxidant defense system, and downregulated growth-related gene expression than the HPC genotype. In ovo feeding of either Met source enhanced the chicks' health status and antioxidant status, and DL-Met improved the hatching weight of the chicks more than L-Met. Genotype differences were significantly evident in the responses of growth performance, antioxidant status, blood biochemical parameters, and gene expression to Met sources.
Variation in maternal lactation practices associated with changes in diurnal maternal inflammation
Authors: Hove Carmen , Chua Kristine Joy , Martin Melanie Ann , Hubble Madison , Boddy Amy M. 
Published: February 2024

AbstractWhile the importance of human milk in shaping infant immune function is well established, the impact of at-the-nipple (ATN) breastfeeding on maternal immune status has been understudied. Since lactation evolved to support infant survival and boost maternal fitness, we predict that ATN breastfeeding will confer benefits on maternal immune function. We measure the absolute and relative frequency of different infant feeding methods (ATN breastfeeding, pumping, donated milk, other supplementation) used by postpartum women in Seattle, WA (USA). We implement Bayesian modeling to estimate the effects of ATN breastfeeding on diurnal change in secretion rate of “pro-inflammatory” salivary cytokines and C-reactive protein (CRP). Our results show that most mothers in our sample used a variety of infant feeding methods, with pumping as the most common alternative to ATN breastfeeding. We find that ATN breastfeeding is associated with non-linear effects on diurnal IL-8 and CRP. Furthermore, we find that women who report zero versus ubiquitous ATN breastfeeding exhibit opposing diurnal patterns in CRP secretion rate. This study provides evidence that variation in maternal lactation practices corresponds to differences in maternal immune responses, highlighting how measuring lactation as a continuous variable can further enhance understanding of postpartum maternal physiology.
Polyunsaturated fatty acids-induced ferroptosis suppresses pancreatic cancer growth
Authors: Suda Akane , Umaru Banlanjo Abdulaziz , Yamamoto Yui , Shima Hiroki , Saiki Yuriko , Pan Yijun , Jin Liang , Sun Jiaqi , Low Yi Ling Clare , Suzuki Chitose , Abe Takaaki , Igarashi Kazuhiko , Furukawa Toru , Owada Yuji , Kagawa Yoshiteru 
Published: February 2024

AbstractDespite recent advances in science and medical technology, pancreatic cancer remains associated with high mortality rates due to aggressive growth and no early clinical sign as well as the unique resistance to anti-cancer chemotherapy. Current numerous investigations have suggested that ferroptosis, which is a programed cell death driven by lipid oxidation, is an attractive therapeutic in different tumor types including pancreatic cancer. Here, we first demonstrated that linoleic acid (LA) and α-linolenic acid (αLA) induced cell death with necroptotic morphological change in MIA-Paca2 and Suit 2 cell lines. LA and αLA increased lipid peroxidation and phosphorylation of RIP3 and MLKL in pancreatic cancers, which were negated by ferroptosis inhibitor, ferrostatin-1, restoring back to BSA control levels. Similarly, intraperitoneal administration of LA and αLA suppresses the growth of subcutaneously transplanted Suit-2 cells and ameliorated the decreased survival rate of tumor bearing mice, while co-administration of ferrostatin-1 with LA and αLA negated the anti-cancer effect. We also demonstrated that LA and αLA partially showed ferroptotic effects on the gemcitabine-resistant-PK cells, although its effect was exerted late compared to treatment on normal-PK cells. In addition, the trial to validate the importance of double bonds in PUFAs in ferroptosis revealed that AA and EPA had a marked effect of ferroptosis on pancreatic cancer cells, but DHA showed mild suppression of cancer proliferation. Furthermore, treatment in other tumor cell lines revealed different sensitivity of PUFA-induced ferroptosis; e.g., EPA induced a ferroptotic effect on colorectal adenocarcinoma, but LA or αLA did not. Collectively, these data suggest that PUFAs can have a potential to exert an anti-cancer effect via ferroptosis in both normal and gemcitabine-resistant pancreatic cancer.
Assessment of ownership of smart devices and the acceptability of digital health data sharing
Authors: Shandhi Md Mobashir Hasan , Singh Karnika , Janson Natasha , Ashar Perisa , Singh Geetika , Lu Baiying , Hillygus D. Sunshine , Maddocks Jennifer M. , Dunn Jessilyn P. 
Published: February 2024

AbstractSmart portable devices- smartphones and smartwatches- are rapidly being adopted by the general population, which has brought forward an opportunity to use the large volumes of physiological, behavioral, and activity data continuously being collected by these devices in naturalistic settings to perform research, monitor health, and track disease. While these data can serve to revolutionize health monitoring in research and clinical care, minimal research has been conducted to understand what motivates people to use these devices and their interest and comfort in sharing the data. In this study, we aimed to characterize the ownership and usage of smart devices among patients from an expansive academic health system in the southeastern US and understand their willingness to share data collected by the smart devices. We conducted an electronic survey of participants from an online patient advisory group around smart device ownership, usage, and data sharing. Out of the 3021 members of the online patient advisory group, 1368 (45%) responded to the survey, with 871 female (64%), 826 and 390 White (60%) and Black (29%) participants, respectively, and a slight majority (52%) age 58 and older. Most of the respondents (98%) owned a smartphone and the majority (59%) owned a wearable. In this population, people who identify as female, Hispanic, and Generation Z (age 18–25), and those completing higher education and having full-time employment, were most likely to own a wearable device compared to their demographic counterparts. 50% of smart device owners were willing to share and 32% would consider sharing their smart device data for research purposes. The type of activity data they are willing to share varies by gender, age, education, and employment. Findings from this study can be used to design both equitable and cost-effective digital health studies, leveraging personally-owned smartphones and wearables in representative populations, ultimately enabling the development of equitable digital health technologies.
Establishment and characterization of turtle liver organoids provides a potential model to decode their unique adaptations
Authors: Zdyrski Christopher , Gabriel Vojtech , Gessler Thea B. , Ralston Abigail , Sifuentes-Romero Itzel , Kundu Debosmita , Honold Sydney , Wickham Hannah , Topping Nicholas E. , Sahoo Dipak Kumar , Bista Basanta , Tamplin Jeffrey , Ospina Oscar , Piñeyro Pablo , Arriaga Marco , Galan Jacob A. , Meyerholz David K. , Allenspach Karin , Mochel Jonathan P. , Valenzuela Nicole 
Published: February 2024

AbstractPainted turtles are remarkable for their freeze tolerance and supercooling ability along with their associated resilience to hypoxia/anoxia and oxidative stress, rendering them an ideal biomedical model for hypoxia-induced injuries (including strokes), tissue cooling during surgeries, and organ cryopreservation. Yet, such research is hindered by their seasonal reproduction and slow maturation. Here we developed and characterized adult stem cell-derived turtle liver organoids (3D self-assembled in vitro structures) from painted, snapping, and spiny softshell turtles spanning ~175My of evolution, with a subset cryopreserved. This development is, to the best of our knowledge, a first for this vertebrate Order, and complements the only other non-avian reptile organoids from snake venom glands. Preliminary characterization, including morphological, transcriptomic, and proteomic analyses, revealed organoids enriched in cholangiocytes. Deriving organoids from distant turtles and life stages demonstrates that our techniques are broadly applicable to chelonians, permitting the development of functional genomic tools currently lacking in herpetological research. Such platform could potentially support studies including genome-to-phenome mapping, gene function, genome architecture, and adaptive responses to climate change, with implications for ecological, evolutionary, and biomedical research.
Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium
Authors: Sun Zhengwu , Lu Kun , Kamla Christine , Kameritsch Petra , Seidel Thomas , Dendorfer Andreas 
Published: February 2024

AbstractDysfunctional Ca2+ signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca2+ and force is essential to investigate the relationship between contractility and Ca2+ signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca2+ and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca2+ were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca2+ signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.
Nav1.8-expressing neurons control daily oscillations of food intake, body weight and gut microbiota in mice
Authors: Bullich-Vilarrubias Clara , Romaní-Pérez Marina , López-Almela Inmaculada , Rubio Teresa , García Carlos J. , Tomás-Barberán Francisco A. , Sanz Yolanda 
Published: February 2024

AbstractRecent evidence suggests a role of sensory neurons expressing the sodium channel Nav1.8 on the energy homeostasis control. Using a murine diphtheria toxin ablation strategy and ad libitum and time-restricted feeding regimens of control or high-fat high-sugar diets, here we further explore the function of these neurons on food intake and on the regulation of gastrointestinal elements transmitting immune and nutrient sensing.The Nav1.8+ neuron ablation increases food intake in ad libitum and time-restricted feeding, and exacerbates daily body weight variations. Mice lacking Nav1.8+ neurons show impaired prandial regulation of gut hormone secretion and gut microbiota composition, and altered intestinal immunity.Our study demonstrates that Nav1.8+ neurons are required to control food intake and daily body weight changes, as well as to maintain physiological enteroendocrine and immune responses and the rhythmicity of the gut microbiota, which highlights the potential of Nav1.8+ neurons to restore energy balance in metabolic disorders.
Enteric and systemic postprandial lactate shuttle phases and dietary carbohydrate carbon flow in humans
Authors: Leija Robert G. , Curl Casey C. , Arevalo Jose A. , Osmond Adam D. , Duong Justin J. , Huie Melvin J. , Masharani Umesh , Brooks George A. 
Published: February 2024

AbstractDietary glucose in excess is stored in the liver in the form of glycogen. As opposed to direct conversion of glucose into glycogen, the hypothesis of the postprandial lactate shuttle (PLS) proposes that dietary glucose uptake is metabolized to lactate in the gut, thereby being transferred to the liver for glycogen storage. In the present study, we provide evidence of a PLS in young healthy men and women. Overnight fasted participants underwent an oral glucose tolerance test, and arterialized lactate concentration and rate of appearance were determined. The concentration of lactate in the blood rose before the concentration of glucose, thus providing evidence of an enteric PLS. Secondary increments in the concentration of lactate in the blood and its rate of appearance coincided with those of glucose, which indicates the presence of a larger, secondary, systemic PLS phase driven by hepatic glucose release. The present study challenges the notion that lactate production is the result of hypoxia in skeletal muscles, because our work indicates that glycolysis proceeds to lactate in fully aerobic tissues and dietary carbohydrate is processed via lactate shuttling. Our study proposes that, in humans, lactate is a major vehicle for carbohydrate carbon distribution and metabolism.
Unveiling a novel metal-to-metal transition in LuH2: Critically challenging superconductivity claims in lutetium hydrides
Authors: Wang Dong , Wang Ningning , Zhang Caoshun , Xia Chunsheng , Guo Weicheng , Yin Xia , Bu Kejun , Nakagawa Takeshi , Zhang Jianbo , Gorelli Federico , Dalladay-Simpson Philip , Meier Thomas , Lü Xujie , Sun Liling , Cheng Jinguang , Zeng Qiaoshi , Ding Yang , Mao Ho-kwang 
Published: February 2024

Following the recent report by Dasenbrock-Gammon et al. [Nature 615, 244–250 (2023)] of near-ambient superconductivity in nitrogen-doped lutetium trihydride (LuH3−δNε), significant debate has emerged surrounding the composition and interpretation of the observed sharp resistance drop. Here, we meticulously revisit these claims through comprehensive characterization and investigations. We definitively identify the reported material as lutetium dihydride (LuH2), resolving the ambiguity surrounding its composition. Under similar conditions (270–295 K and 1–2 GPa), we replicate the reported sharp decrease in electrical resistance with a 30% success rate, aligning with the observations by Dasenbrock-Gammon et al. However, our extensive investigations reveal this phenomenon to be a novel pressure-induced metal-to-metal transition intrinsic to LuH2, distinct from superconductivity. Intriguingly, nitrogen doping exerts minimal impact on this transition. Our work not only elucidates the fundamental properties of LuH2 and LuH3, but also critically challenges the notion of superconductivity in these lutetium hydride systems. These findings pave the way for future research on lutetium hydride systems, while emphasizing the crucial importance of rigorous verification in claims of ambient-temperature superconductivity.
How is the relationship between rainfall and water vapor in the Indian monsoon influenced by changes in lapse rate during global warming?
Authors: Mallesh Rakshith , Srinivasan Jayaraman 
Published: February 2024

Abstract
Most climate models have shown that the Indian Summer Monsoon Rainfall (ISMR) will increase on account of global warming. The primary reason is the increase in column water vapor (CWV). The rainfall increase is not, however, proportional to the increase in column water vapor; for a given amount of CWV, rainfall will be lower in the future, according to model simulations. This suggests that other there are factors are at play. We have used a diagnostic model based on moist static energy (MSE) and moisture conservation to examine the impact of changes in the vertical lapse rate of temperature. Our results indicate that on decadal time scales, changes in ISMR are mediated by changes in CWV and temperature lapse rates. This is consistent with previous studies that showed the impact of column-mean temperature on the rainfall-CWV relationship. Findings are significant for climate model projections of ISMR, as correct estimation of lapse rate changes in models is necessary to predict ISMR changes accurately.
Investigation on the Compressive Characteristics and Optimization of Design Parameters of a Novel Functionally Graded Cell Structure
Authors: Ganapathy Sakthi Balan , Sakthivel Aravind Raj 
Published: February 2024

Abstract
Novel structural conceptualizations frequently incorporate inventive ideas, materials, or construction techniques. This study presents a unique design inspired by the traditional practice of sikku rangoli, a cultural tradition prevalent in the southern region of India, particularly in Tamil Nadu. Because it was novel, it was necessary to optimize the fundamental design for maximal outputs. In contrast to honeycomb structures, intercellular interactions are believed to contribute to the overall strengthening of the structure. By eliminating sharp corners from the structure, stress accumulation is prevented, resulting in improved stress distribution. Therefore, the design aspects that were deemed significant were taken into consideration and through the implementation of experimental design, an optimum design was determined. Utilizing the optimal base design as a foundation, the structure underwent several printing processes using diverse materials and incorporated multiple fillers. Furthermore, the structure was subjected to modifications employing the functional grading design concept. The study employed the functional grading design concept to examine the variations in load bearing capability, load distribution, and failure mode. The findings indicate that the compression strength of the composite structure was mostly influenced by the wall thickness. The combination of a carbon fiber reinforced base material with silicone rubber as filler, together with a functional graded cell structure featuring top and bottom densification, exhibited the highest compression strength compared to all other combinations. In order to investigate the accurate impact of the FG structures, every cell design was printed using PLA-CF, subjected to testing devoid of any additives, and the output parameters were computed. The results indicated that the center densified cell design exhibited significant values for specific energy absorption, relative density, and compressive strength (52.63 MPa, 0.652, and 2.95 kJ kg−1, respectively). The design of the base cell exhibited the greatest crushing force efficacy of 0.982.
Pixelated non-volatile programmable photonic integrated circuits with 20-level intermediate states
Authors: Chen Wenyu , Liu Shiyuan , Zhu Jinlong 
Published: February 2024

Abstract
Multi-level programmable photonic integrated circuits (PICs) and optical metasurfaces have gained widespread attention in many fields, such as neuromorphic photonics, optical communications, and quantum information. In this paper, we propose pixelated programmable Si3N4 PICs with record-high 20-level intermediate states at 785 nm wavelength. Such flexibility in phase or amplitude modulation is achieved by a programmable Sb2S3 matrix, the footprint of whose elements can be as small as 1.2 μm, limited only by the optical diffraction limit of an in-house developed pulsed laser writing system. We believe our work lays the foundation for laser-writing ultra-high-level (20 levels and even more) programmable photonic systems and metasurfaces based on phase change materials, which could catalyze diverse applications such as programmable neuromorphic photonics, biosensing, optical computing, photonic quantum computing, and reconfigurable metasurfaces.