bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles
Authors: Li Kun , Cui Yadong , Zheng Xue , Min Chunyan , Zhang Jian , Yan Zhanpeng , Ji Yu , Ge Fei , Ji Hualiang , Zhu Fangshi 
Published: January 2024

Abstract

Objective
Immunological liver injury (ILI) is a common liver disease associated with the microbiota-gut-liver axis. Jian Gan powder (JGP) has protective and therapeutic effects on hepatitis virus-induced ILI in the clinic; nonetheless, underlying mechanisms are unclear.We aim to investigate the hepatoprotective effect of JGP in a mouse model of ILI.
Methods
The mouse model was established using Bacillus Calmette-Guérin (BCG) plus lipopolysaccharide (LPS). After JGP treatment (7.5, 15, or 30 g/kg), serum, liver, and fresh fecal samples were analyzed. 16S rRNA gene sequencing and untargeted metabolomics profiling were performed to assess the role of JGP on the gut microbiota and its metabolites.
Results
JGP treatment markedly reduced serum IFN-γ, IL-6, IL-22, and hepatic p-STAT3 (phosphorylated transducer and activator of transcription-3) expression. In contrast, JGP increased the percentage of proliferating cell nuclear antigen-positive liver cells in treated mice. Fecal 16S rRNA gene sequencing revealed that JGP treatment restored the levels of Alloprevotella, Burkholderia-Caballeronia-Paraburkholderia, Muribaculum, Streptococcus, and Stenotrophomonas. Additionally, metabolomics analysis of fecal samples showed that JGP restored the levels of allylestrenol, eplerenone, phosphatidylethanolamine (PE) (P-20:0/0:0), sphingomyelin (SM) d27:1, soyasapogenol C, chrysin, and soyasaponin I.
Conclusions
JGP intervention improves ILI by restoring gut microbiota and modifying its metabolic profiles. These results provide a novel insight into the mechanism of JGP in treating ILI and the scientific basis to support its clinical application.
Antibiotic prophylaxis during dental implant surgery treatment in Northwest China: A cross-sectional study
Authors: Lin Yao , Huang Jing , Yue Jiao , Cheng Lifei , Ma Yanfei , Pan Wen , Ju Jia , Wei Hongbo , Feng Bin 
Published: January 2024

Abstract

Background This study was designed to investigate the existing status of antibiotic prophylaxis during dental implant surgery in northwest China. Methods This cross-sectional study was conducted based on a web survey following the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. The questionnaire comprised the basic information of respondents, the awareness of antibiotic prophylaxis and the antimicrobial prescription habits of dentists performing dental implants. The questionnaire was sent individually to 2099 dentists who performed dental implants in northwest China from April to December of 2021. Data were collected and analyzed. Results A total of 318 participants responded survey. Of the respondents, 65.41% (n = 208) routinely prescribed antibiotics during dental implant surgery, while only 0.94% (n = 3) did not prescribe them at all. A total of 141 (44.40%) respondents reported concerns regarding the indications for antibiotic use. The most frequently used antibiotics included penicillins, cephalosporins, and nitroimidazoles. Respondents inclined to prolong the regimens of antibiotics routinely for more than 24 h, specifically in patients with bone grafts,which reached to 85.08%. Conclusions Antibiotics are widely used to prevent infection during dental implant surgery in northwest China. Dentists are inclined to prolong the course of antibiotics routinely both in healthy individuals and patients with bone grafts.
Strength properties and water retention curve of unsaturated Berea sandstone during drying
Authors: Togashi Yota , Kosunago Yuki , Osada Masahiko , Asamoto Shingo , Hatakeyama Ken 
Published: January 2024

Abstract
A decrease in rock strength due to water content increase and water transfer in them must be considering while discussing the deformation of rock slopes and tunnels.A water retention curve is necessary to describe the strength properties and water transport for unsaturated rocks.Although the wetting curve can be obtained through mercury intrusion porosimetry test, generating the drying curve is difficult owing to the large suction. It is preferable to be able to identify the drying suctions in other ways.In this study, strength changes of Berea sandstone during drying due to water content change are shown,and a method for obtaining the water retention drying curve using strengths is presented. The degree of saturation in the specimens for both tests was was adjusted during the drying process by air drying in our laboratory room.The Mohr--Coulomb failure criteria were then obtained from the Brazilian and uniaxial compression test results. The results demonstrated that the shear resistance angle was almost constant regardless of the water content (degree of saturation), and only the cohesion decreased with increase in water content.Since this indicates the change in mean stress (suction) due to water content changes, suction can be identified from the Brazilian and uniaxial compression tests.Furthermore, Bayesian estimation of the van Genuchten parameters was performed using the suction determined by mean stress changes detected by series of loading tests. As expected, the obtained drying curve was larger than the wetting curve obtained by mercury intrusion porosimetry test.
Study on the asymmetric distribution characteristics of striatal dopamine transporter in early Parkinson's disease
Authors: Li Xiuming , Wang Ruifang , Huang Qi , Tan Haibo 
Published: January 2024

Abstract
Background
To exploring the characteristics of asymmetric distribution of dopamine transporter(DAT) in the striatum of early Parkinson's disease (PD) patients using 11C-2β-carbomethoxy-3β-(4-fluorophenyl)-tropane (11C-CFT) positron emission tomography/computed tomography (PET/CT) imaging.
Methods
We recruited 20 patients with early PD and 12 age- and gender-matched healthy controls for 11C-CFT PET/CT imaging. For each subject, the striatum was displayed on the maximum slice of 11C-CFT PET/CT images. Volumes of interest (VOIs) were manually outlined on the caudate, anterior and posterior putamen, and occipital cortex. The specific binding ratio (SBR), asymmetry index (AI), and the anterior-posterior gradient of striatal DAT uptake were calculated based on the mean standardized uptake value (SUVmean) within each VOI.
Results
The SBR of DAT in the anterior and posterior putamen, as well as in the bilateral caudate, was significantly lower in the early PD patients compared to the control group, especially on the contralateral side (p < 0.05). Compared to the control group, the asymmetry index and the anterior-posterior gradient of striatal DAT uptake were significantly higher in the striatal subregions of early PD patients, with the posterior putamen showing the greatest reduction, followed by the anterior putamen, and finally the caudate, indicating a trend of gradual development from posterior to anterior.
Conclusions
11C-CFT PET imaging can accurately demonstrate the characteristics of presynaptic dopaminergic neuron dysfunction in the striatum of early PD patients, providing imaging basis for the diagnosis and pathogenesis research of early PD.
The epigenomic consequences of HDAC6 inactivation reveal a crucial role for P300 suggesting integrated epitherapeutic strategies
Authors: Zamperla Michela Gottardi , Illi Barbara , Barbi Veronica , Cencioni Chiara , Santoni Daniele , Gagliardi Stella , Garofalo Maria , Zingale Gabriele Antonio , Pandino Irene , Sbardella Diego , Cipolla Lina , Sabbioneda Simone , Farsetti Antonella , Ripamonti Chiara , Fossati Gianluca , Steinkühler Christian , Gaetano Carlo , Atlante Sandra 
Published: January 2024

Abstract
Background: Histone deacetylases (HDACs) play a pivotal role in gene regulation, DNA synthesis, and cellular metabolism, significantly influencing cancer development. Class IIb histone deacetylase 6 (HDAC6) is crucial in maintaining protein stability and regulating chromatin dynamics, with notable implications in cancer and immune responses. Despite this, the specific effects of HDAC6 inactivation on gene regulation and chromatin remodeling remain unclear due to the subtle phenotype changes following its genetic deactivation. This study delves into how inactivating HDAC6 affects the stabilization of lysine acetyltransferase P300 and the subsequent impacts on chromatin structure and function in cancer cells.
Methods & Results: Using the HDAC6 inhibitor ITF3756 and CRISPR/Cas9 gene editing, we deactivated HDAC6 in various cancer cell lines. We observed profound changes in chromatin accessibility, particularly in the acetylation of histone H3 lysines 9, 14, and 27, which accumulated into introns and distal intergenic regions as determined through ATAC-seq and H3K27Ac ChIP-seq analyses. Transcriptomics, proteomics, and gene ontology analysis revealed alterations in gene function linked to cell proliferation, adhesion, migration, and apoptosis. A notable finding was the modification of P300 ubiquitination post HDAC6 inactivation, which increased P300 expression and activity, resulting in the downregulation of genes essential for cellular proliferation and survival.
Conclusions: This research underscores the significant effect of HDAC6 inactivation on the chromatin landscape in cancer cells, shedding light on the crucial role of P300 in the HDAC6-mediated anticancer response. Specifically, the role of P300 stabilization in the effectiveness of an HDAC6 inhibitor suggests for a potential shift in focus from HDAC6 to its interaction with P300. This insight could contribute to developing more precise cancer treatments by targeting this specific pathway, thereby enhancing our knowledge of cancer cell behavior and offering new therapeutic perspectives.
Barriers and Facilitators to Health-seeking Behaviors and Self-care Practices of Older Adults in Rural India, A Mixed Method Study
Authors: M Yogesh , Makwana Naresh , Damor Naresh , Trivedi Nidhi 
Published: January 2024

Abstract
Background: Effective self-care is crucial for maintaining health in older adults in resource-constrained communities. This study examined self-care practices, health-seeking behaviors, and associated factors among older adults in rural India.
Methods: A mixed-methods study was conducted among 400 adults aged ≥65 years. Participants were selected through multistage random sampling from 5 slum areas. Self-care practices, health-seeking behaviors, demographic information, chronic conditions, self-efficacy, and health literacy were assessed through interviews. The qualitative data were explored through in-depth interviews with 30 participants.
Results: Inadequate health literacy 180 (45%) and low self-efficacy 150 (37.5%) were common. While 300 (75%) reported an adequate diet and 350 (87.5%) reported medication adherence, only 80 (20%) monitored diabetes complications. Only 180 (45%) patients underwent cancer screening, and 300 (75%) had ≥2 doctor visits yearly. Age, sex, education, income, comorbidities, self-efficacy, and health literacy were significantly associated with self-care and health-seeking. Along with facilitators such as social support, barriers such as limited healthcare access and lack of preventive orientation emerged.
Conclusion: Suboptimal prevention orientation and overreliance on secondary care instead of self-care among elderly people are problematic given the limited use of geriatric services. Grassroots health workers can improve health literacy and self-efficacy through home visits to enable self-care. Healthcare access inequities for vulnerable groups merit policy attention.
Functional identification of CCR1 gene in apple (Malus halliana) demonstrates that it enhances saline-alkali stress tolerance
Authors: Wang Xiu , Zhang Zhongxing , wang Wanxia , Li SiTian , Li JuanLi , wang Yanxiu 
Published: January 2024

Abstract
Background
Lignin is a complex aromatic polymer that plays an important biological role in maintaining plant structure and defending plants against biotic and abiotic stresses. Cinnamoyl-CoA reductase (CCR) is a key enzyme involved in the lignin synthesis-specific pathway and regulates lignin biosynthesis and accumulation.
Methods
Based on transcriptome data, MhCCR1, which was significantly induced by saline-alkali stress, was cloned from Malus halliana. The physicochemical properties, evolutionary relationships and cis-acting elements were analyzed. Subsequently, the tolerance of overexpressed MhCCR1 in Arabidopsis thaliana, tobacco and apple calli to saline-alkali stress was verified by genetic transformation. And yeast two-hybridization technique was applied to screen and validate the interacting proteins.
Results
We found that overexpression of MhCCR1 enhanced the tolerance of A. thaliana, tobacco and apple calli under saline-alkali stress, and caused a variety of physiological and biochemical changes. As compared to the wild type, the transgenic plants showed better growth, higher lignin, chlorophyll and proline contents, lower conductivity and MDA content, and significant increase in antioxidant enzyme activities (SOD, POD, CAT) in the transgenic lines under stress condition. In addition, expression of saline-alkali stress-related genes in overexpressed A. thaliana were also higher than in WT, including the antioxidant genes, the Na+ transporter genes, and the H+-ATPase genes, while expression of the K+ transporter genes displayed opposite changes. Meanwhile, the expression levels of genes related to lignin synthesis, AtPAL1, AtCOMT, AtC4H, At4CL1, and AtCCOAOMT, were also significantly up-regulated. At last, the Y2H experiment confirmed the interaction between MhCCR1 and MhMYB4, MhMYB1R1, MhHXK, and MhbZIP23 proteins.
Conclusions
These results suggest that MhCCR1 may play a positive regulatory role in saline-alkali tolerance of transgenic lines by regulating the lignin content, osmoregulatory substances, chlorophyll content, antioxidant enzyme activities, and genes related to saline-alkali stress, thus providing excellent resistance genes for the stress-responsive regulatory network of apples, and providing a theoretical basis for the cultivation of saline and alkali resistant apple varieties.
Synthesis, structure, and catalytic behaviour for oxygen reduction of a µ2-OH-bridged binuclear cobalt complex
Authors: Li Can-Hao , Li Wei , Zhan Shu-Zhong 
Published: January 2024

Abstract
The reaction of 2-pyridylamino-N,N-bis(2-methylene-4,6-dichlorophenol) (H2L) with CoCl2 affords a binuclear cobalt complex, [L-Co-µ2(OH)2-Co-L], which has been determined by using single crystal X-ray diffraction. Electrochemical studies show that this cobalt complex can serve as a molecular catalyst for oxygen reduction reaction (ORR). Under − 0.4 V vs Ag/AgCl, the H2O2 yield is 83% with 0.20 M KOH. Moreover, investigations exbibit that in homogeneous environments dissociation of a OH− ion from the binuclear cobalt complex makes positions on cobalt centres, letting dioxygen (O2) inserted and reduced. We hope these findings can provide a new ideal for the developments of electrocatalysts for ORR.
Association between dietary patterns and diabetic kidney disease in patients with type 2 diabetes mellitus and a mediational analysis: a cross-sectional study
Authors: Liu Yijun , Li Ping , Li Jing , Xu Linxin , He Yasha , Qiao Jing , Guan Zhaojuan , Zhang Yanbo , Wang Yan 
Published: January 2024

Abstract
Aims
This study aimed to investigate the relationship between different dietary patterns and diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM) and explore the mediating effect produced by blood glucose levels.
Materials and Methods
This study was conducted according to the China Chronic Disease and Risk Factors Surveillance (CCDRFS). Two districts and two counties were randomly selected from CCDRFS in Shanxi Province to collect general information, dietary records, physical measurements, and laboratory tests. 1,332 patients were enrolled.
Results
The prevalence of DKD was 21.9%, which was lower in urban than in rural areas. Dietary patterns were constructed: 'animal protein', 'coarse grains and plant protein', 'nuts and fruits', 'refined grains and vegetables', 'dairy', and 'added sugars', with factor contributions of 15.4%, 10.0%, 8.3%, 8.1%, 7.6%, and 7.2% respectively, explaining 56.6% of the total dietary variation. Patients in the highest quartile of dietary pattern scores for 'nuts and fruits' experienced a 50.5% lower risk of albuminuria and 'added sugars' showed a 3.123-fold increase risk of reduced estimated glomerular filtration rate (eGFR) compared with those in the lowest quartile. The 'nuts and fruits' dietary pattern negatively affected UACR through the mediation of fasting plasma glucose (FPG) and haemoglobin A1c (HbA1c), with a total effect value of -0.019.
Conclusions
In T2DM, the 'nuts and fruits' dietary pattern decreased the risk of albuminuria, and the 'added sugars' dietary pattern increased the risk of decreased eGFR. FPG and HbA1c partially mediated the association between the 'nuts and fruits' dietary pattern and DKD.
Influenza surveillance in pigs: balancing act between broad diagnostic coverage and specific virus characterization
Authors: Stadler Julia , Zwickl Sophia , Gumbert Sophie , Ritzmann Mathias , Lillie-Jaschniski Kathrin , Harder Timm , Graaf-Rau Annika , Skampardonis Vassilis , Eddicks Matthias 
Published: January 2024

Abstract

Background
Monitoring of infectious diseases on swine farms requires a high diagnostic sensitivity and specificity of the test system. Moreover, particularly in cases of swine Influenza A virus (swIAV) it is desirable to include characterization of the virus as precisely as possible. This is indispensable for strategies concerning prophylaxis of swIAV and furthermore, to meet the requirements of a purposeful monitoring of newly emerging IAV strains in terms of vaccine design and public health. Within the present cross-sectional study, we compared the diagnostic value of group samples (wipes of surfaces with direct contact to mouth/nose, dust wipes, udder skin wipes, oral fluids) to individual samples (nasal swabs, tracheobronchial swabs) for both swIAV identification and characterization. Sampling included different stages of pig production on 25 sow farms with attached nursery considered as enzootically infected with swIAV. Firstly, samples were analyzed for IAV genome and subsequently samples with Ct-values < 32 were subtyped by multiplex RT-qPCR.
Results
Nasal swabs of suckling piglets and nursery pigs resulted in a higher odds to detect swIAV (p < 0.001) and to identify swIAV subtypes by RT-qPCR (p < 0.05) compared to nasal swabs of sows. In suckling piglets, nasal swabs and sow udder skin wipes were significantly more often swIAV positive compared to contact wipes from the farrowing unit (p = 0.007; p = 0.036). In the nursery, group sampling specimens yielded higher rates of swIAV detection compared to individual samples. However, in general nasal swabs were more likely to have Ct-value < 32 and thus, to be suitable for subtyping by RT-qPCR compared to dust wipes, contact wipes, udder skin wipes and tracheobronchial swabs (p < 0.05). Despite the high detection rate of swIAV in dust wipes, those specimens had the lowest odds of identifying subtypes by RT-qPCR (p < 0.05). Interestingly, different subtypes were found in different age groups as well as in different specimens in the same holding.
Conclusion
Although population-based specimens are highly effective for swIAV monitoring, nasal swabs are still the preferable sampling material for the surveillance of on-farm circulating strains due to significantly higher virus loads. Remarkably, sampling strategies should incorporate suckling piglets and different age groups within the nursery to cover all on-farm circulating strains.
Colonoscopy Utilization in Elderly Dementia Patients: Characteristics, Complications, and Charges in a National Matched-Cohort Analysis
Authors: Shweikeh Faris , Hong Gordon , Rogers Kathleen , Mouchli Mohamad , Hoscheit Matthew , Lembo Anthony 
Published: January 2024

Abstract
Background
It is projected that the elderly population will continue to increase. Many will develop chronic conditions such as dementia.
Aims
Our aims are to describe the utilization of colonoscopy among dementia patients and compare outcomes in those with and without dementia.
Methods
This population-based analysis utilized the National Inpatient Sample (NIS) during 2019. Patients with dementia over the age of 60 years receiving colonoscopy were identified utilizing the Elixhauser comorbidity measure and ICD-10 codes. Propensity score matching was used to correct for effects of uneven features of the clinical outcomes. Multivariate regression analysis was used to assess the effect of patient factors.
Results
Initially, 50,692 patients without dementia were compared with 4,323 patients with dementia. Dementia patients were more likely to be female, older, less likely white, had lower income, and more likely to be on Medicare. In the matched comparison (4,176 in each group), complications analysis showed dementia patients did not have higher colonoscopy-related complications. They did have higher rates of other complications including renal/AKI (p = 0.0042), pulmonary/pneumonia (p = 0.003), cerebrovascular accidents (p = 0.0063), and sepsis (< 0.0001). Dementia patients were also less likely to have routine discharges (< 0.0001), had longer hospital stays (< 0.0001), and higher hospital costs (< 0.0001).
Conclusions
Elderly dementia patients have similar colonoscopy-related complications as patients without dementia. However, they do have higher complications in general. The decision whether to perform colonoscopy in this patient population is multifactorial. A careful assessment of a dementia patient’s history can help with this decision.
One-step fabricated ZnO electron-transporting layers for perovskite light-emitting diodes with sub-bandgap turn-on voltage
Authors: SUN XINZHI , BAI JIALIN , WANG TING , ZHANG HANZHUANG , JI WENYU 
Published: January 2024

Abstract

Efficient charge injection into the emitters, which can reduce the voltage loss at interfaces, is a prerequisite for high-performance light-emitting diodes, with low voltage operation. Here we develop a sol-gel ZnO (s-ZnO) electron-transport layer for the perovskite light-emitting diodes (PeLEDs) with green-emission formamidinium lead bromide (FAPbBr3) perovskites as emitters. Polyethylenimine (PEI) is mixed into the s-ZnO precursor as a modifier, which not only promotes the wettability of s-ZnO films, but also passivates the defects of s-ZnO without sacrificing their electrical conductivity. As a result, highly efficient FAPbBr3 films are obtained on the s-ZnO films. The maximum current efficiency of PeLED with s-ZnO:PEI electron-transporting layer reaches 13.5 cd/A, 45% higher than that based on pristine s-ZnO without PEI modifier. Benefiting from the outstanding charge-transport properties of s-ZnO and high-quality perovskite film, the turn-on voltage of the s-ZnO based PeLEDs is only 1.9 V, much lower than the band-gap voltage (~ 2.3 V) of FAPbBr3.