bluuebunny/crossref_metadata_2025_split huggingface dataset:
#118 : The “HERAQoL-P”: Development of a Meaningful, International Quality of Life Tool for PCOS
Authors: Avery Jodie , Pirotta Stephanie , Jiskoot Geranne , Helm Melanie Gibson 
Published: January 2024

Background and Aims: Women with Polycystic Ovary Syndrome (PCOS) have diverse experiences of Quality of Life (QoL) depending on their age, ethnicity, socio economic status, severity and phenotype. PCOS, a common endocrine condition in women, exhibits problematic symptoms related to fertility, weight gain, mental health and sleep. QoL is an important health outcome, yet standard measures include predefined items, that may be unimportant to individuals. The full physical, emotional, and social impact of PCOS is not captured with existing PCOS QoL measures. These factors need to be addressed within person-centred care in order to achieve the best possible health outcomes in both the short and long term. We aim to scope and understand priorities for quality of life (QoL) dimensions from women with PCOS throughout life, to provide a conceptual framework for the creation of a meaningful, scientifically validated quality of life tool. Method: Based on the Schedule for the Evaluation of Individual Quality of Life (SEIQoL) questionnaire, we surveyed 80 women and interviewed 17 women diagnosed with PCOS in Australia, with another 93 completing the survey in the Netherlands, about aspects of life important for their quality of life. Interview questions include the impact of this condition on physical, mental and emotional health, and understand whether PCOS affects women differently according to their symptoms and background profile. Results: This work informs the development of a new QoL tool which will be internationally validated, reflecting the diverse PCOS features with the greatest impact, enabling better evaluation of Patient Reported Outcomes. Conclusion: A high-quality QoL questionnaire could highlight women’s specific priorities, and then measure whether their healthcare addresses such priorities. We will then create the HERAQoL-P, with extensive potential to have impact, in both research and clinical care, adding to the evidence base for PCOS.
#147 : Artificial Intelligence is Equally Capable of Predicting Viability of Embryos Created from Vitrified Oocytes, Despite Observed Differences in Morphokinetic Behaviour
Authors: Pereira Gabriella , Sorby Kelli 
Published: January 2024

Background and Aims: With oocyte vitrification increasingly employed and the ever-expanding presence of artificial intelligence (AI) in the laboratory, it is essential to understand whether the same weight can be given to AI models’ predictions when vitrified oocytes are utilized. Method: Embryos were cultured in Embryoscope+ and assigned an iDA score, generated after 112 hours in culture. Data was collected from 186 vitrified oocyte (VO) cycles using a total of 1577 vitrified oocytes, along with control data from 43,957 fresh oocytes. Data analysed included all standard morphokinetic parameters, iDA scores, embryo utilisation and clinical pregnancy. Statistical analyses were performed using Logistic Regression, Fisher’s exact test and unpaired t-tests. Results: The majority of cycles utilised clinic-recruited donor oocytes (70.7%), followed by autologous (25.9%) and known-donor oocytes (3.4%). No difference was observed in blastocyst utilisation between the three groups (48.0%, 48.1% and 52.9% respectively). In patients undertaking a single cycle utilising both fresh and vitrified oocytes, blastocyst utilisation was significantly higher in fresh oocytes (58.98% vs. 41.12%, p<0.0001), however, no difference in clinical pregnancy rate was observed (40.0% vs. 40.5%). Fresh and vitrified oocytes displayed significantly different morphokinetic behaviour across all time points assessed. The magnitude of difference was greatest during compaction and blastulation, with VO embryos compacting on average 6.53 hours later than control embryos (p<0.0001) and commencing blastulation 4.58 hours later (p<0.0001). When analysing iDA scores against the clinical pregnancy rate of fresh vs. vitrified oocytes, logistic regression showed no difference in areas under the curve (AUC) between the two groups (0.6431 vs. 0.6390, 95% Confidence Intervals 0.6111–0.6751 and 0.5474-0.7300 respectively), demonstrating no reduction in the AI’s ability to predict embryo viability despite their morphokinetic differences. Conclusion: AI technology predicted clinical pregnancy equally well whether embryos originated from fresh or vitrified oocytes, despite confirmation of significantly delayed morphokinetic parameters in embryos formed from vitrified oocytes.
#228 : Variability of Symptom Profiles in Individuals with Mate Kirikoopu/Endometriosis and/or Huahua Hua Kuao/Polycystic Ovary Syndrome in Aotearoa New Zealand
Authors: Salemink-Waldren Jamie , Adamson Zachary , Bush Deborah , Campbell Rebecca , Hohmann-Marriott Bryndl , Johnson Neil , Nisa-Waller Arianna , Pankhurst Michael , Girling Jane 
Published: January 2024

Background and Aims: Although opposing risk factors have been proposed for mate kirikōpū/endometriosis (oestrogen-dependence) and huahua hua kūao/polycystic ovary syndrome (PCOS; androgen dependence), there is a cohort of individuals with both endometriosis and PCOS. We propose that disease comorbidity will alter symptom profiles in comparison to those with only one disease, and that analysis of these differential profiles may hint at underlying mechanisms of disease pathology. Methods: An anonymous online survey was distributed via social media (NZ residents, >18 years) and included questions related to diagnosis, symptoms, treatment and management of endometriosis and/or PCOS. Results: In total, 1323 responses (median [range] = 31 years [18–72]) were received within a two-week period (endometriosis only: n=615, 48%; PCOS only: n=459, 35%; both diseases: n=247, 19%). When asked about symptoms ‘ever experienced’, comorbid individuals report both symptoms commonly ascribed to endometriosis (e.g, dysmenorrhea) as well as those ascribed to PCOS (e.g., irregular periods) thereby exacerbating impacts on quality of life. An exception, the proportion of individuals reporting absent menstrual periods was increased in cormobid individuals (45%) relative to those with endometriosis only (21%), but decreased relative to those with PCOS only (67%), suggesting a more complex interaction between the two diseases. Conclusions: These data highlight the heterogenity of disease phenotype in individuals with endometriosis and/or PCOS. We suggest that an understanding of the intersection of these two common diseases, and a recognition of the associated phenotypic diversity, will provide avenues of research to better understand the pathophysiology of both endometriosis and PCOS.
#312 : Outcomes for Women Who Utilise Electively Frozen Oocytes
Authors: Dalton Anna , Pacela-Ince Leanne , Koch Juliette 
Published: January 2024

Background and Aims: Frozen oocyte thaw (FOT) cycles are becoming increasingly common within IVF clinics in Australia, as women choose to freeze eggs and delay using those eggs until a more suitable time. Typically, Australian women freezing eggs are older than those women who have been studied in overseas FOT cycles. The aim of this study was to report outcomes of FOT cycles. Method: We performed a retrospective cohort study of 141 FOT cycles (from 129 patients) over a 10-year period from January 2010 to December 2020, at a single IVF clinic in Adelaide, Australia. We assessed the outcomes of those cycles in terms of oocyte warm survival, ICSI fertilisation rates, utilisation rates, pregnancy rates and live birth rates. Results: The average age of women undergoing FOT cycles was 36.5 ± 4.9 years. The reasons for egg freezing cycles included male factor (45.5%), elective (44.1%), religious (6.3%), fertility preservation (2.8%) and anonymous oocyte donation (1.4%). From the 143 FOT cycles, 1094 oocytes were collected; 82% of oocytes survived the warm process and 62% fertilized normally. There were 59 fresh embryo transfers (5.1% cleavage and 94.9% blastocyst, 22.0% DET and 78% SET) and 32 frozen blastocyst embryo transfers (12.5% DET and 87.5% SET). The pregnancy rate per fresh transfer was 30.0% (18/59) and per frozen transfer was 37.5% (12/32). The live birth rate per fresh transfer was 27% (16/59) and per frozen transfer was 37.5% (12/32). The live birth rate per initiated FOT cycle was 11% (16/143). Conclusion: Rates of live birth per initiated FOT cycle at our clinic was 11%, similar to the ANZARD live birth rate for all fresh cycles initiated in 2020 (10.9%). We therefore conclude that results for frozen oocytes are equivalent to fresh oocytes and can use this information when counselling patients on the outcomes of FOT cycles.
#87 : Intrauterine Adhesion in Ultrasound-Guided Manual Vacuum Aspiration (USG-MVA) Versus Electric Vaccum Aspiration (EVA): A Randomized Controlled Trial
Authors: Ng Karen , Chung Pui Wah Jacqueline , Law Sze Man , Ip Patricia Nga Ping , Li Tin Chiu 
Published: January 2024

Background and Aims: Intrauterine adhesion (IUA) is a possible complication after uterine surgery, especially after sharp curettage for miscarriages as it destroys the endometrial stratum basalis. This study aimed to determine and compare the IUA in women who underwent ultrasound-guided manual vacuum aspiration (USG-MVA) as opposed to traditional surgical evacuation (EVA) for the management of their first-trimester miscarriage. Methods: This was a prospective single-centre, randomised controlled trial conducted in a university-affiliated tertiary hospital. Chinese women aged [Formula: see text]18 years with a delayed miscarriage [Formula: see text]12 weeks of gestation or incomplete miscarriage attending the hospital were randomized to either USG-MVA or EVA for the management of their miscarriage. They were invited to come back for a hysteroscopic assessment for IUA at 6-20 weeks post-surgery to assess the incidence of IUA. Patients were contacted by phone at 6 months to assess their menstrual and reproductive outcomes. Results: 303 patients underwent surgical evacuation, of whom 152 were randomized to the ‘USG-MVA’ and 151 patients to the ‘EVA’. 126 from the USG-MVA group and 125 from the EVA group returned and completed the hysteroscopic assessment. The incidence of the IUA from USG-MVA was 19.0% (n=24/126) and was significantly lower compared to EVA, which was at 32.0% (n=40/125). There was no significant difference in menstrual outcomes at 6 months postoperatively between the two groups, but more patients had miscarriages in the EVA group with IUA. Conclusions: IUAs are a possible complication of USG-MVA. However, USG-MVA is associated with a lower incidence of IUA postoperatively at 6-20 weeks. USG-MVA is a feasible, effective, and safe alternative surgical treatment with less IUA for the management of first-trimester miscarriage.
#357 : Uterine Fibroids Treatment Using Radio Frequency Ablation at Hanoi Obstetrics and Gynecology Hospital, Vietnam: A Preliminary Assessment
Authors: Ahn Dao Le Thi , Van Vu Duong , Toan Thang Vuong , Duy Anh Nguyen 
Published: January 2024

Complicated uterine fibroids in young women, who wish to preserve fertility, and/ or unwilling to undergo surgery, or those having uterine fibroids deemed difficult for surgical intervention, need a less invasive and effective treatment method. Objectives: To evaluate the results of treatment of uterine fibroids by Radio Frequency Ablation (RFA) at Hanoi Obstetrics and Gynecology Hospital, Vietnam (HOGH) for 8 months. Materials and Methods: A cross-sectional study with longitudinal follow-up. Patients with symptomatic uterine fibroids including abnormal uterine bleeding, pelvic pain, anemia; in need of preserving fertility; without any RFA contra-indications. Results: 18 patients with 20 fibroids were met with the study’s inclusion criteria. The average fibroid size was 4.6±1.3 cm. 77,8% of patients having anemia. Average thermal ablation time was 12.2±4.1 minutes. Fibroids’ size decreased 43.6% after 1 month, 69.6% after 3 months and 83.6% after 6 months. Menstrual disorders and pelvic pain were considerably improved after 1 month and 3 months. Conclusions: RFA had good initial results in reducing fibroids size and improving relating symptoms during the study period.
#378 : Intrauterine Suture of Levonorgestrel Intrauterine Device Under Hysteroscopy
Authors: Shi Xiaoyu , Zhang Shengpeng , Feng Limin 
Published: January 2024

Background and Aims: Levonorgestrel-releasing intrauterine system (LNG-IUS) is an effective and long-term treatment for adenomyosis, especially for patients who have severe dysmenorrhea symptoms but a strong desire to preserve their uterus. However, expulsion of the device for patients with an enlarged uterus remains a problem. In this study, an LNG-IUS was stitched on the posterior wall of the uterus under hysteroscopy, which can be an alternative method to this problem. Methods: 21 patients with adenomyosis who had previously been treated with the LNG-IUS and experienced expulsion for at least one time were enrolled. The fixation of the LNG-IUD on the posterior wall of the uterus was performed with a 4-0 Ethicon polypropylene non-absorbable suture through an endoscopic needle driver and a knot-pushing device. Results: During the postoperative follow-up, all patients experienced complete remission of their symptoms: a significant decrease in menstrual flow, relief of dysmenorrhea, and improvement in quality of life. No expulsion of device and other compilations were found. Conclusions: In patients with adenomyosis suffering from dysmenorrhea or heavy menstrual blood loss, suture fixation of an LNG-IUS using the hysteroscopic cold knife surgery system is a minimally invasive and effective alternative treatment for patients with an enlarged uterus and decreases the risk of LNG-IUS expulsion.
#120 : Effect of Aromatase Inhibitor, Letrozole on Semen Parameters in Eugonadotropic Hypoandrogenic Men with Oligozoospermia
Authors: Jahan Israt 
Published: January 2024

Background and Aims: Some men with severely defective sperm production commonly have excess aromatase activity, reflected by low serum testosterone and relatively elevated estradiol level. Elevated levels of estrogen lead to feedback inhibition of the hypothalamic-pituitary-gonadal axis, and the end result is a decrease in LH necessary for the production of testosterone and FSH to optimize sperm production. The aim of the study was to evaluate the effect of aromatase inhibitor, letrozole on the semen parameters of infertile male having oligozoospermia, low serum testosterone level and testosterone to estradiol ratio < 10. Method: This prospective study was conducted in the Department of Reproductive Endocrinology and Infertility at Bangabandhu Sheikh Mujib medical University, Dhaka, Bangladesh during the period from May 2019 to April 2020. The sample size was 30 sub fertile men with oligozoospermia (<-10 million/ml), low serum testosterone level (<300 ng/dl), and low testosterone to estradiol ratio (<10). All patients were treated with letrozole 2.5 mg daily for 4 months. At the end of the 4 months semen analysis was performed. Statistical analysis was carried out by using IBM Statistical Package for Social Sciences version 25 for windows. Results: The sperm concentration, sperm motility and total motile sperm count significantly increased after letrozole treatment. The side effects were mild and well tolerated. Conclusion: This study suggests that some men with oligozoospermia low serum testosterone levels and normal gonadotropin concentration may have a treatable endocrinopathy. Letrozole may be used to improve sperm parameters in infertile men with low serum testosterone to estradiol ratio.
#268 : Assessment of Seminal Micro-Biota and Its Impact on Semen Parameters Using Next-Generation Sequencing
Authors: Vajpeyee Manisha , Tiwari Shivam , Yadav Lokendra , Chimote Nishad 
Published: January 2024

Background and Aims: To assess seminal microbiome and its impact on semen parameters, Male genitourinary tract infections are believed to affect reproductive health and affect semen quality. Despite a possible link between bacteria and infertility, few studies have attempted to characterize the seminal microbiota in healthy and diseased subjects. Methods: The microbial composition was analysed by 16S ribosomal RNA (rRNA) gene amplification with (MinION) oxford Nanopore Ltd. In a cohort of infertility-related cases (N = 166) and controls (N = 69). Results: Diverse kinds of bacteria were present in most of the samples from infertile patients with normal and abnormal semen parameters, the bacterial communities varied widely between subjects, however, statistical tests showed a significant negative association between sperm quality and the presence of certain pathogenic bacteria. In seminal hyper-viscosity group had a lower abundance of the probiotic’s lactobacillus and a higher abundance of infections Proteobacteria. In addition, the principal component analysis (PCA) revealed a difference in microbial composition between the normal and abnormal groups, as well as a larger microbial abundance in hyper-viscosity and oligoasthenospermia; samples compared to control groups. Conclusions: We intend to describe seminal microbe’s role in reproduction as well as association with male infertility. Our results suggested that microbes play an important role in our life as they can have negative as well as positive impact of semen associated with male factor fertility.
#327 : The Characteristics of Menopausal Women in Moewardi Hospital: What Factors Could Lead to the Depression?
Authors: Jatiningtyas Abida Zuhra , Nugroho Christa Edo , Wijayanti Agung Sari , Budihastuti Uki Retno , Melinawati Eriana 
Published: January 2024

Background and Aims: A Woman may begin to experience several symptoms during the premenopausal and menopausal stages, including depression. Some studies have linked the emergence of various symptoms, including emotional changes, to hormonal changes. Several factors could lead to depression, such as the knowledge and the behaviour of premenopausal and menopausal women, nutritional status, duration of menopause, access to health care, and many more. The primary goal of this study was to determine the relationship between menopause and depression by examining the subjects’ Hamilton Depression Rating Scale (HAM-D) results and some potentially related characteristics. Method: This research was held in Moewardi Hospital, Surakarta, Indonesia between July until December 2022, involving participants women aged 40-60 years old, totaling 60 people. Thematic analysis of semi-structured interviews based on the HAM-D was employed in this study. We examined the characteristics such as age, duration of menopause, body mass index, hypertension, anxiety, and activities. The data were collected from women aged 40 and over, in the premenopausal or menopausal phase, who agreed to be interviewed and gave informed consent and analysed with IBM SPSS Statistic 23. Results: Statistical tests showed that there was no correlation between depression and a woman’s age (p=0.404), duration of menopause (p=0.846), body mass index (p=0.339), and hypertension (p=0.909). We found significant results of depression with anxiety (p<0.001) as well as patients’ activities (p<0.001). These two characteristics were included in the HAM-D and then grouped into five categories according to the HAM-D. This questionnaire gave results weak correlation between depression and the menopausal phase (p=0.176). Conclusion: Several factors could lead to the incidence of depression in premenopausal and menopausal women. This study found that there was a correlation between depression and anxiety and patient’s activity.
#134 : Unraveling the mTORC1 Mediated Pathway for Reproductive Longevity Rejuvenation by Targetting RPS6
Authors: Tan Xue Ting , Teh Daniel BL , Lau Lang-Chu , Sim Weiling Eunice , Lee Jovin , Tang Richard , Lee Jonathan , Lee Jin-Young , Deng Shuo , Yap Celestial Theresa , Tan Tuan Zea , Ong Wei Yi , Pan Feng , Tan Xing Fei , Yip George W. , Huang Zhongwei 
Published: January 2024

Background and Aims: The mTORC1 pathway is known to play a critical role in longevity and is a central regulator for various pathways including cellular, inflammation, and metabolic pathways. Age-related functional decline in tissue was associated with dysregulation of the mTORC1 pathway. mTORC1 suppression has been shown to improve ovarian function in mice, but the underlying cause remains elusive. We aim to investigate a common denominator of mTOR1, ribosomal protein S6 (rpS6), and its role in reproductive longevity through transient inhibition of the rps6 axis using murine models. Method: Selective inhibition of the mTORC1 pathway and the rpS6 axis were achieved using rapamycin and LY2584702 Tosylate respectively. Subcutaneous injection of both drugs was administered to 48-week-old female virgin C57BL/6J mice for thrice weekly for 8 weeks. Reproductive outcomes such as mating frequencies, fecundity, and lifespan were recorded. Results: Reproductive parameters measured revealed that these 48-week-old female virgin treated mice resulted in the increase in serum Anti-Mullerian hormone levels, total number of ovarian follicles, mating frequencies and probability of live births. Untreated control mice at 62 weeks old did not yield any live births. Comparison between the two treatment groups suggested that selective inhibition of rpS6 demonstrated higher probability of live births and higher average number of live offspring per litter. Conclusion: This study provides novel insights into the roles of mTORC1 and rpS6 in both reproductive and healthy longevity. Our findings suggest temporary rpS6 inhibition successfully rejuvenated ageing ovaries, with the novel discovery in the improvement of fecundity of LY2584702 treated mice.
#320 : Metabolic Risks in Women with Polycystic Ovary Syndrome Diagnosed by the Rotterdam Criteria Versus the New International Criteria
Authors: Li Raymond Hang Wun , Ng Ernest Hung Yu 
Published: January 2024

Background and Aims: In the Rotterdam criteria (2003), polycystic ovary morphology for diagnosing polycystic ovary syndrome (PCOS) was defined as an antral follicle count (AFC) of 12 or more (or ovarian volume of 10 cc or more) in either ovary. In the international guideline (2018), the AFC threshold was increased to 20. We compared the metabolic risks in women with PCOS diagnosed by these two ultrasound criteria. Method: We prospectively recruited 467 women with PCOS diagnosed by the Rotterdam criteria, and 240 healthy ovulatory women as controls. Of those in the PCOS cohort, 142 had AFC of 12 to 19 (Rotterdam criteria group), while 325 had AFC of 20 or above in either ovary (international criteria group). The prevalence rates of dysglycaemia and metabolic syndrome (MetS) (and its constituent components) at baseline were compared by chi-squared test. The cumulative hazard of dysglycaemia and MetS were compared between the two groups by Kaplan-Meier survival analysis. Results: Compared to controls, women in both groups had significantly higher (p<0.05) prevalence of dysglycaemia, MetS, elevated waist circumference, reduced HDL-cholesterol and elevated fasting glucose. In addition, those in the international criteria group had a significantly higher (p<0.05) prevalence of hypertriglyceridaemia and hypertension. Upon longitudinal follow-up censored at the sixth completed year, 23 (16.3%) women in the Rotterdam criteria group versus 80 (24.8%) women in the international criteria group had dysglycaemia (p=0.044), with a hazard ratio of 1.560 (95% CI 1.005-2.389) (p=0.048). Meanwhile, 33 (23.2%) and 96 (29.7%) women in the Rotterdam and international criteria groups respectively had MetS (p=0.151), with a hazard ratio of 1.343 (95% CI 0.910-1.984) (p=0.138). Conclusion: Women with PCOS, either diagnosed by the AFC threshold of the Rotterdam criteria or international criteria, are both at elevated risks of dysglycaemia and MetS, with the latter group demonstrating an exaggerated risk of dysglycaemia.