bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serine/threonine/tyrosine phosphorylation of the LHX3 LIM‐homeodomain transcription factor
Authors: Parker Gretchen E. , West Brooke E. , Witzmann Frank A. , Rhodes Simon J. 
Published: November 2004

AbstractLHX3 is a LIM homeodomain transcription factor with essential roles in pituitary and motor neuron development in mammals. Patients with mutations in the LHX3 gene have combined pituitary hormone deficiency and other symptoms. In this study, we show that the LHX3 protein can be modified post‐translationally by phosphorylation. LHX3 can serve as a substrate for protein kinase C and casein kinase II. Overexpression of these kinases reduces the transcriptional capacity of LHX3 to activate target genes. Following exposure of LHX3 to cellular kinases, mass spectrometry was used to map the phosphorylation of five amino acid residues within the human LHX3a isoform. Two phosphorylated residues (threonine 63 and serine 71) lie within the first LIM domain of the protein. Three other modified amino acids (tyrosine 227, serine 234, and serine 238) are located in the carboxyl terminus. Targeted replacement of these amino acids with non‐modifiable residues significantly reduced the ability of LHX3 to activate both synthetic and pituitary hormone reporter genes. However, the amino acid replacements did not significantly affect the capability of LHX3 to interact with the NLI, PIT1, and MRG1 partner proteins, or its ability to bind to a high affinity DNA site. In conclusion, we have identified unique amino acids within LHX3 that are important for its transcriptional activity and are phosphorylated. © 2004 Wiley‐Liss, Inc.
On strategies for enforcing interfacial constraints and evaluating jump conditions with the extended finite element method
Authors: Ji H. , Dolbow J. E. 
Published: November 2004

AbstractWe consider a problem stemming from recent models of phase transitions in stimulus‐responsive hydrogels, wherein a sharp interface separates swelled and collapsed phases. Extended finite element methods that approximate the local solution with an enriched basis such that the mesh need not explicitly ‘fit’ the interface geometry are emphasized. Attention is focused on the weak enforcement of the normal configurational force balance and various options for evaluating the jump in the normal component of the solute flux at the interface. We show that as the reciprocal interfacial mobility vanishes, it plays the role of a penalty parameter enforcing a pure Dirichlet constraint, eventually triggering oscillations in the interfacial velocity. We also examine alternative formulations employing a Lagrange multiplier to enforce this constraint. It is shown that the most convenient choice of basis for the Lagrange multiplier results in oscillations in the multiplier field and a decrease in accuracy and rate of convergence in local error norms, suggesting a lack of stability in the discrete formulation. Under such conditions, neither the direct evaluation of the gradient of the approximation at the phase interface nor the interpretation of the Lagrange multiplier field provide a robust means to obtain the jump in the normal component of solute flux. Fortunately, the adaptation and use of local, domain‐integral methodologies considerably improves the flux evaluations. Several example problems are presented to compare and contrast the various techniques and methods. Copyright © 2004 John Wiley & Sons, Ltd.
Leadership in Lean, Empowering Manufacturing Organizations
Authors: Carroll Bob 
Published: November 2004

AbstractGoing from a traditional, command‐and‐control organizational structure to an empowered workforce is not an easy transformation. Here is how one manager did it in a sophisticated work environment, as he introduced short‐cycle manufacturing. It required new skills on the part of the manager as well as the employees. © 2001 John Wiley & Sons, Inc.
Partition of protein solvation into group contributions from molecular dynamics simulations
Authors: Morreale Antonio , de la Cruz Xavier , Meyer Tim , Gelpí Josep Lluís , Luque F. Javier , Orozco Modesto 
Published: November 2004

AbstractLinear response theory coupled to molecular dynamics simulations with an explicit solvent representation is used to derive fractional contributions of amino acid residues to the solvation of proteins. The new fractional methods developed here are compared with standard approaches based on empirical 1D and 3D statistical potentials, as well as with estimates obtained from the analysis of classical molecular interaction potentials. The new fractional methods, which have a clear physical basis and explicitly account for the effects due to protein structure and flexibility, provide an accurate picture of the contribution to solvation of different regions of the protein. Proteins 2005. © 2004 Wiley‐Liss, Inc.
Theoretical study of energies, structures, and vibrational spectra of the carbonic acid–hydroperoxy radical complexes
Authors: Qu Yuhui , Bian Xiufang , Tang Hongwei , Si Pengchao 
Published: November 2004

AbstractIn this work, we present the results for the first time of our study on hydrogen‐bonded H2CO3–HO2 complexes (structures 1, 2) by means of ab initio molecular orbital theory. These complexes are important intermediates in the reaction of the hydroperoxy radical and cabonic acid. We calculated that these structures are a six‐membered ring. We found that the binding energy of two complexes are 5.8 and 9.3 kcal/mol using the CCSD(T) method. We also calculated the vibrational and rotational frequencies for these complexes. © 2004 Wiley Periodicals, Inc. Int J Quantum Chem, 2005
Quantitative determination of the novel anticancer drug E7070 (indisulam) and its metabolite (1,4‐benzenedisulphonamide) in human plasma, urine and faeces by high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry
Authors: Beumer Jan Hendrik , Rosing Hilde , Hillebrand Michel J. X. , Nan‐Offeringa Lianda G. A. H. , Foley Karen , Yule S. Murray , Heck Albert J. R. , Schellens Jan H. M. , Beijnen Jos H. 
Published: November 2004

AbstractE7070 (indisulam) is a novel anticancer drug currently undergoing clinical investigation. We present a sensitive and specific method for the quantitative determination of E7070 and its metabolite M1 (1,4‐benzenedisulphonamide) in human plasma, urine and faeces. The analytes and their tetra‐deuterated analogues, which were used as internal standards, were isolated from the biological matrix by solid‐phase extraction with OASIS cartridges (0.5 mL plasma or 1 mL urine) and by liquid‐liquid extraction with ethyl acetate at pH 5 (1 mL faecal homogenate). The analytes were separated on a C8 reversed‐phase chromatographic column and analyzed using electrospray ionization and tandem mass spectrometric detection in the negative ion mode. The validated concentration ranges in plasma were 0.1–20 μg/mL for E7070 and 0.01–2 μg/mL for M1. In urine and faecal homogenate, a concentration range from 0.05–10 μg/mL or μg/g, respectively, was validated for both analytes. Validation of the plasma assay was performed according to the most recent FDA guidelines. The assay fulfilled all generally accepted requirements for linearity (r > 0.99, residuals between −8 and 10%), accuracy (−13.5 to 1.4%) and precision (all less than 11%) in the tested matrices. We investigated recovery, stability (working solutions at −20°C and at room temperature, biological matrices at −20°C, room temperature and after 3 freeze/thaw cycles; final extracts at room temperature) and robustness. All these parameters were found acceptable. This method is suited for mass balance studies or therapeutic drug monitoring, as demonstrated by a case example showing plasma concentrations and cumulative excretion of E7070 and M1 in urine and faeces. Furthermore, we show the presence of E7070 metabolites in patient urine. Copyright © 2004 John Wiley & Sons, Ltd.
On‐line analysis of diesel engine exhaust gases by selected ion flow tube mass spectrometry
Authors: Smith David , Španĕl Patrik , Dabill David , Cocker John , Rajan Bob 
Published: November 2004

AbstractSelected ion flow tube mass spectrometry (SIFT‐MS) has been used to analyse on‐line and in real time the exhaust gas emissions from a Caterpillar 3304 diesel engine under different conditions of load (idle and 50% of rated load) and speed (910, 1500 and 2200 rpm) using three types of fuel: an ultra‐low‐sulphur diesel, a rapeseed methyl ester and gas oil. SIFT‐MS analyses of the alkanes, alkenes and aromatic hydrocarbons in the headspace of these fuels were also performed, but the headspace of the rapeseed methyl ester consists mainly of methanol and a compound with the molecular formula C4H8O. The exhaust gases were analysed for NO and NO2 using O reagent ions and for HNO2 using H3O+ reagent ions. The following aldehydes and ketones in the exhaust gases were quantified by using the combination of H3O+ and NO+ reagent ions: formaldehyde, acetaldehyde, propenal, propanal, acetone, butanal, pentanal, butanone and pentanone. Formaldehyde, acetaldehyde and pentenal, all known respiratory irritants associated with sensitisation to asthma of workers exposed to diesel exhaust, are variously present within the range 100–2000 ppb. Hydrocarbons in the exhaust gases accessible to SIFT‐MS analyses were also quantified as total concentrations of the various isomers of C3H4, C3H6, C4H6, C5H8, C5H10, C6H8, C6H10, C7H14, C6H6, C7H8, C8H10 and C9H12. Copyright © 2004 John Wiley & Sons, Ltd.
Reference ranges for umbilical and middle cerebral artery pulsatility index and cerebroplacental ratio in prolonged pregnancies
Authors: Palacio M. , Figueras F. , Zamora L. , Jiménez J. M. , Puerto B. , Coll O. , Cararach V. , Vanrell J. A. 
Published: November 2004

AbstractObjectiveTo construct normal ranges for umbilical artery pulsatility index (UA PI), middle cerebral artery pulsatility index (MCA PI) and cerebroplacental ratio (CPR) in prolonged pregnancies according to strict methodological criteria using polynomial regression analysis.MethodsThis was a retrospective, cross‐sectional observational study involving 140 women, 10 women for each gestational day between 287 and 300 days of gestation. Fetal Doppler parameters were assessed to construct normal reference ranges for UA PI and MCA PI. CPR was calculated as a ratio of MCA PI/UA PI.ResultsMathematical modeling of the data demonstrated that the optimal fit was a linear polynomial one. Mean, 5th and 95th centiles were calculated for UA, MCA and CPR and centile curves from the regression analysis were constructed.ConclusionsReference ranges for UA PI, MCA PI and CPR in prolonged pregnancies have been constructed. MCA PI shows a wider range than previously reported in the literature. Copyright © 2004 ISUOG. Published by John Wiley & Sons, Ltd.
Comparison of three expression systems for heterologous xylanase production byS. cerevisiaein defined medium
Authors: Görgens Johann F. , Planas Jordi , van Zyl Willem H. , Knoetze Johannes H. , Hahn‐Hägerdal Bärbel 
Published: November 2004

AbstractThe influence of the auxotrophic deficiencies of the host strain and expression vector selection on the production of a heterologous protein was investigated. Heterologous xylanase production by two prototrophicS. cerevisiaetransformants, containing either a plasmid‐based, YEp‐type expression system or an integrative, YIp‐type expression system, were compared with production by an auxotrophic transformant, containing an identical YEp‐type expression system, in batch and continuous cultivation, using a chemically defined medium. Heterologous xylanase production by the auxotrophic strains in defined medium was critically dependent on the availability of amino acids, as extracellular xylanase production increased dramatically when amino acids were over‐consumed from the medium to the point of saturating the cell. Saturation with amino acids, indicated by an increased leakage of amino acids from the cell, was thus a prerequisite for high level of heterologous protein production by the auxotrophic strain. Maximal xylanase production levels by the auxotrophic strain corresponded to the levels obtained with a similar prototrophic strain during cultivation in defined medium without amino acids. Superfluous auxotrophic markers thus had a strong deleterious effect on heterologous protein production by recombinant yeasts, and the use of such strains should be limited to initial exploratory investigations. The increased copy number and foreign gene dosage of the YEp‐based expression vector, stabilized by theura3 fur1autoselection system, significantly improved production levels of heterologous xylanase, compared to the YIp system, which is based on a single integration into the yeast genome. No evidence was found of the possible saturation of the host secretory capacity by multicopy overexpression. Stable production of heterologous xylanase at high levels by the prototrophic YEp‐based recombinant strain, compared to the YIp system, was demonstrated. Copyright © 2004 John Wiley & Sons, Ltd.
Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence
Authors: Nicolescu G. , Linton Y.-M. , Vladimirescu A. , Howard T.M. , Harbach R.E. 
Published: November 2004

AbstractMosquitoes of the Anopheles maculipennis group were collected in five districts of Romania (Constanta, Giurgiu, Ilfov, Mehedinti and Suceava) between March 2000 and June 2003. Two hundred and ninety-seven specimens were identified by molecular methods. Nuclear rDNA ITS2 sequences of 178 specimens were compared with GenBank sequences for nine known Palaearctic species of the group, and 119 specimens were identified using an ITS2 PCR–RFLP assay developed during the study. Five genetically distinct species of the group were identified: A. atroparvus van Thiel, A. maculipennis Meigen, A. melanoon Hackett and A. messeae Falleroni and a previously unrecognized species. The new species, herein formally described and named A. daciaesp. n., was collected in the Black Sea coastal region and plains adjacent to the Danube River in southern Romania. Anopheles daciae is most similar to and sympatric with A. messeae. It is contrasted with A. messeae and characterized on the basis of unique nuclear ITS2 and mitochondrial COI DNA sequences and morphological characters of the eggs. The larval, pupal and adults stages of the two species were also compared, but no reliable characters were found to distinguish them. It seems likely that A. daciae is more widespread in eastern Europe and the Balkan States, and could be responsible for malaria transmission in these regions that is currently attributed to A. messeae. Anopheles melanoon is reported from Romania for the first time.
Melatonin promoted chemotaxins expression in lung epithelial cell stimulated with TNF-α
Authors: Luo FengMing , Liu XiaoJing , Li ShuangQing , Liu ChunTao , Wang ZengLi 
Published: November 2004

Abstract
Background
Patients with asthma demonstrate circadian variations in the airway inflammation and lung function. Pinealectomy reduces the total inflammatory cell number in the asthmatic rat lung. We hypothesize that melatonin, a circadian rhythm regulator, may modulate the circadian inflammatory variations in asthma by stimulating the chemotaxins expression in the lung epithelial cell.

Methods
Lung epithelial cells (A549) were stimulated with melatonin in the presence or absence of TNF-α(100 ng/ml). RANTES (Regulated on Activation Normal T-cells Expressed and Secreted) and eotaxin expression were measured using ELISA and real-time RT-PCR, eosinophil chemotactic activity (ECA) released by A549 was measured by eosinophil chemotaxis assay.

Results
TNF-α increased the expression of RANTES (307.84 ± 33.56 versus 207.64 ± 31.27 pg/ml of control, p = 0.025) and eotaxin (108.97 ± 10.87 versus 54.00 ± 5.29 pg/ml of control, p = 0.041). Melatonin(10-10 to 10-6M) alone didn't change the expression of RNATES (204.97 ± 32.56 pg/ml) and eotaxin (55.28 ± 6.71 pg/ml). However, In the presence of TNF-α (100 ng/ml), melatonin promoted RANTES (410.88 ± 52.03, 483.60 ± 55.37, 559.92 ± 75.70, 688.42 ± 95.32, 766.39 ± 101.53 pg/ml, treated with 10-10, 10-9, 10-8, 10-7,10-6M melatonin, respectively) and eotaxin (151.95 ± 13.88, 238.79 ± 16.81, 361.62 ± 36.91, 393.66 ± 44.89, 494.34 ± 100.95 pg/ml, treated with 10-10, 10-9, 10-8, 10-7, 10-6M melatonin, respectively) expression in a dose dependent manner in A549 cells (compared with TNF-α alone, P < 0.05). The increased release of RANTES and eotaxin in A549 cells by above treatment were further confirmed by both real-time RT-PCR and the ECA assay.

Conclusion
Taken together, our results suggested that melatonin might synergize with pro-inflammatory cytokines to modulate the asthma airway inflammation through promoting the expression of chemotaxins in lung epithelial cell.
Effect of dietary palm olein oil on oxidative stress associated with ischemic-reperfusion injury in isolated rat heart
Authors: Narang Deepak , Sood Subeena , Thomas Mathew Kadali , Dinda Amit Kumar , Maulik Subir Kumar 
Published: November 2004

Abstract
Background
Palm olein oil (PO), obtained from refining of palm oil is rich in monounsaturated fatty acid and antioxidant vitamins and is widely used as oil in diet in many parts of the world including India. Palm oil has been reported to have beneficial effects in oxidative stress associated with hypertension and arterial thrombosis. Oxidative stress plays a major role in the etiopathology of myocardial ischemic-reperfusion injury (IRI) which is a common sequel of ischemic heart disease. Antioxidants have potent therapeutic effects on both ischemic heart disease and ischemic-reperfusion injury. Information on the effect of PO on ischemic-reperfusion injury is, however, lacking. In the present study, the effect of dietary palm olein oil on oxidative stress associated with IRI was investigated in an isolated rat heart model. Wistar rats (150–200 gm) of either sex were divided into three different groups (n = 16). Rats were fed with palm olein oil supplemented commercial rat diet, in two different doses [5% v / w (PO 5) and 10% v / w (PO 10) of diet] for 30 days. Control rats (C) were fed with normal diet. After 30 days, half the rats from each group were subjected to in vitro myocardial IRI (20 min of global ischemia, followed by 40 min of reperfusion). Hearts from all the groups were then processed for biochemical and histopathological studies. One way ANOVA followed by Bonferroni test was applied to test for significance and values are expressed as mean ± SE (p < 0.05).

Results
There was a significant increase in myocardial catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities with no significant change in myocardial thiobarbituric acid reactive substances (TBARS) only in group PO 5 as compared to group C. There was no light microscopic evidence of tissue injury. A significant rise in myocardial TBARS and depletion of myocardial endogenous antioxidants (SOD, CAT and GPx) along with significant myocyte injury was observed in control rats subjected to ischemia-reperfusion (C IR). Hearts from palm olein oil fed rats subjected to ischemia-reperfusion (PO 5 IR and PO 10 IR) were protected from increase in TBARS and depletion of endogenous antioxidants as compared to C IR group. No significant myocyte injury was present in the treated groups.

Conclusions
The present study demonstrated for the first time that dietary palm olein oil protected rat heart from oxidative stress associated with ischemic-reperfusion injury.
No effects of GSM-modulated 900 MHz electromagnetic fields on survival rate and spontaneous development of lymphoma in female AKR/J mice
Authors: Sommer Angela M , Streckert Joachim , Bitz Andreas K , Hansen Volkert W , Lerchl Alexander 
Published: November 2004

Abstract
Background
Several reports indicated that non-thermal electromagnetic radiation such as from mobile phones and base stations may promote cancer. Therefore, it was investigated experimentally, whether 900 MHz electromagnetic field exposure influences lymphoma development in a mouse strain that is genetically predisposed to this disease. The AKR/J mice genome carries the AK-virus, which leads within one year to spontaneous development of thymic lymphoblastic lymphoma.

Methods
320 unrestrained female mice were sham-exposed or exposed (each n = 160 animals) to GSM like 900 MHz electromagnetic fields for 24 hours per day, 7 days per week, at an average whole body specific absorption rate (SAR) value of 0.4 W/kg. Animals were visually checked daily and were weighed and palpated weekly. Starting with an age of 6 months, blood samples were taken monthly from the tail. Animals with signs of disease or with an age of about 46 weeks were sacrificed and a gross necropsy was performed.

Results
Electromagnetic field exposure had a significant effect on body weight gain, with higher values in exposed than in sham-exposed animals. However, survival rate and lymphoma incidence did not differ between exposed and sham-exposed mice.

Conclusion
These data do not support the hypothesis that exposure to 900 MHz electromagnetic fields is a significant risk factor for developing lymphoma in a genetically predisposed species, even at a relatively high exposure level.
Late stent thrombosis in the absence of prior intracoronary brachytherapy
Authors: Heller Louis I. , Shemwell Kathie C. , Hug Karen 
Published: November 2004

AbstractLate stent thrombosis has not been reported in the absence of prior coronary brachytherapy. We reviewed our experience in 1,855 consecutive patients who received at least one stent and did not receive coronary brachytherapy. Half of all stent thromboses occurred within the first week and nearly 65% (22) occurred within 15 days. The incidence of stent thrombosis within this traditional time frame was 1.2%. An additional 12 patients, however, presented with stent thrombosis between 33 and 270 days post‐procedure (mean = 72.9 ± 23 days). The true incidence of stent thrombosis was therefore 1.8% (34/1,855). There were three bypass operations, one stroke and two deaths in the late stent thrombosis group. Late stent thrombosis is an unusual but serious complication in patients who have not received coronary brachytherapy. Intracoronary radiation may potentiate a phenomenon that already occurs after stent deployment. Prolonged treatment (6–12 months) with anti‐platelet agents should be considered after percutaneous intervention with coronary stents. Cathet Cardiovasc Intervent 2001;53:23–28. © 2001 Wiley‐Liss, Inc.
Magnetic Properties of Sm2Co17‐xCrx (0 ≤ x ≤ 3.0) Compounds
Authors: Fuquan B. , Wang J. L. , Tegus O. , Dagula W. , Tang N. , Wang W. Q. , Yang F. M. , Brueck E. , de Boer F. R. , Buschow K. H. J. 
Published: November 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Direct Asymmetric Organocatalytic Michael Reactions of α,α‐Disubstituted Aldehydes with β‐Nitrostyrenes for the Synthesis of Quaternary Carbon‐Containing Products.
Authors: Mase Nobuyuki , Thayumanavan Rajeswari , Tanaka Fujie , Barbas Carlos F. III 
Published: November 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
SmI2‐Mediated 3‐exo‐Trig Cyclization of δ‐Oxo‐α,β‐Unsaturated Esters to Cyclopropanols and Derivatives.
Authors: Bezzenine‐Lafollee Sophie , Guibe Francois , Villar Helene , Zriba Riadh 
Published: November 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Regio‐ and Stereoselective Rearrangement of Epoxides to Aldehydes Catalyzed by High‐Valent Metalloporphyrin Complex, Cr(TPP)OTf.
Authors: Suda Kohji , Kikkawa Taketoshi , Nakajima Shin‐ichiro , Takanami Toshikatsu 
Published: November 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.