bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sixteen cases of laparoscopic central pancreatectomy for benign or low‐grade malignant tumours in the pancreatic neck and proximal body
Authors: Xiang Jianqiang , Zhu Jie , Wang Haibiao , Zhou Xinhua , Li Hong 
Published: February 2024

AbstractBackgroundThe purpose of this study is to examine and analyse the outcomes and patient experiences associated with laparoscopic central pancreatectomy.MethodsThe perioperative data of 16 patients who underwent laparoscopic central pancreatectomy were retrospectively analysed at Ningbo Medical Center Lihuili Hospital (Xingning Branch and Eastern Branch) from September 2017 to July 2023.ResultsAll surgical procedures were completed without the need for intraoperative conversion to open surgery. In two cases, intraoperative cholangiography was performed, while in four cases, intraoperative fluoroscopic laparoscopic assistance was utilized. The duration of the operations varied from 160 to 360 min, with an average of 281.75 min. The estimated volume of intraoperative bleeding ranged from 50 to 300 mL, with an average of 113.75 mL. The postoperative pathology results revealed that there were two cases of intraductal papillary mucinous neoplasm, six cases of serous cystic neoplasms, one case of mucinous cystic neoplasm, five cases of solid pseudopapillary neoplasms, and two cases of neuroendocrine tumours. The maximum diameter of the tumours ranged from 3.0 to 5.0 cm, with an average of 3.67 cm. There were no instances of postoperative common bile duct stenosis or biliary leakage. Among the cases, five did not exhibit pancreatic fistula, six experienced biochemical leakage, three had grade B pancreatic fistula, and two had grade C pancreatic fistula.ConclusionLaparoscopic central pancreatectomy, as a method to preserve pancreatic function, entails specific surgical risks and a notable likelihood of postoperative pancreatic fistula, necessitating the expertise of seasoned surgeons for its execution.
A large‐scale study on the prevalence of math anxiety in Qatar
Authors: Megreya Ahmed M. , Al‐Emadi Ahmed A. , Al‐Ahmadi Aisha M. , Moustafa Ahmed A. , Szűcs Denes 
Published: February 2024

AbstractBackgroundMath anxiety (MA) is a worldwide appearing academic anxiety that can affect student mental health and deter students from math and science‐related career choices.MethodUsing the Arabic version of the Modified‐Abbreviated Math Anxiety Scale (m‐AMAS), the prevalence of MA was investigated in a very large sample of students (N = 10093) from grades 7 to 12 in Qatar.ResultsThe results showed a better fit to the original two‐factor model of the m‐AMAS (learning MA and Evaluation MA) than to a single‐factor solution. This two‐factor model was also confirmed in each grade. Notably, the distribution of MA scores was right‐skewed, especially for learning MA. Using the inter‐quartiles ranges, norms for MA were provided: A score of ≤16 indicates low MA whereas a score of ≥30 identifies high MA. Previous studies conducted in Western countries defined high math‐anxious students as those who score above the 90th percentile corresponding to a score of 30 on the m‐AMAS. Using this cut‐off criterion, the current study found that one‐fifth of students in Qatar were highly math‐anxious, with a higher proportion of females than males. We also calculated the percentage of participants selecting each response category for each questionnaire item. Results showed that attending a long math class was the context that elicited the highest levels of learning MA. In contrast, having an unexpected math test was the situation that triggered the highest levels of evaluation MA.ConclusionThe prevalence of MA might vary across different cultures.
Cardiovascular risk factors predict who should have echocardiographic evaluation in long COVID
Authors: Tamariz Leonardo , Ryan Mathew , Marzouka George R. , Bast Elizabeth , Klimas Nancy , Palacio Ana 
Published: February 2024

AbstractBackgroundThe need for echocardiograms among patients with long COVID is debatable. Our aim was to evaluate the prevalence of left ventricular (LV) dysfunction and identify predictors.MethodsWe conducted a cross‐sectional study and included all consecutive patients enrolled in our post‐COVID clinic. We included patients who had an echocardiogram and had no previous known heart disease. We defined LV dysfunction as a low ejection fraction or grade II to grade III diastolic dysfunction on an echocardiogram with evidence of elevated filling pressures. We calculated the prevalence of heart disease and predictors of heart disease using logistic regression.ResultsWe included 217 post‐COVID patients enrolled in the clinic. The prevalence of LV dysfunction is 24%; 95% CI 18–30. Predictors of heart disease include older age and a previous history of hypertension and diabetes or having an intermediate or high ASCVD score. Patients with low ASCVD score did not have low ejection fraction on the screening echocardiograms.ConclusionOur study found a considerable number of patients with LV dysfunction. Older patients with cardiovascular risk factors are at risk of long COVID associated heart disease.
Biochemical characteristics of sorghum cultivars affect life table parameters, feeding performance, and digestive enzyme activities of Helicoverpa armigera
Authors: Bonvari Ali , Hemmati Seyed Ali , Shishehbor Parviz 
Published: February 2024

AbstractThe cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a major insect pest of various agricultural crops worldwide. In this study, the effects of eight sorghum [Sorghum bicolor (L.) Moench, Poaceae] cultivars (Payam, Pegah, Sepideh, Spidfid, Kimia, KGS23, KFS2, and KFS18) on life table parameters, feeding performance, and activities of digestive enzymes in H. armigera were assessed under laboratory conditions. The insects were fed grains of the eight cultivars. Furthermore, the phytochemicals starch, proteins, flavonoids, and phenolic compounds in the grains were quantified to investigate their relationship with ecological and physiological parameters in H. armigera. Our results revealed that H. armigera had the shortest development time when reared on cv. Sepideh and the longest development time when reared on cv. Payam. The insects reached the highest and lowest intrinsic rate of increase (r) on cv. Sepideh and Payam, respectively. The relative growth rate (RGR) and efficiency of conversion of digested food (ECD) of larvae were highest and lowest on cv. Sepideh and Payam, respectively. Larval proteolytic activity was highest on cv. KFS18 and lowest on cv. Sepideh. Concerning phytochemicals, protein content was highest in cv. KFS18 and lowest in cv. Payam and KGS 23. Total phenolic levels were highest in cv. Payam and lowest in cv. Sepideh. According to the correlation analysis, overall, plant protein content was negatively correlated with insect development time, but positively correlated with pupal weight and r. Total phenolic content was positively correlated with insect development time, and negatively correlated with r and RGR. Cluster analysis indicated that cv. Sepideh was the most susceptible host, whereas cv. Payam was the most resistant. Therefore, cv. Payam may be a candidate for use in integrated pest management (IPM) against H. armigera.
“Masculinity is responsibility toward your family”: Masculinity in the shadow of Bedouin patriarchy in Israel
Authors: Alhuzail Nuzha Allassad , Segev Einav 
Published: February 2024

AbstractObjectiveThe aim of this study is to examine how the patriarchal structure affects Bedouin men's role in the family and how this role affects their masculinity.BackgroundHow the patriarchal structure affects Bedouin women and constrains them has been studied extensively, but there is a dearth of research on how it affects young Bedouin men—especially those who are the sons of men with higher education—who have been influenced by modernization and exposure to Israeli society.MethodThis is a qualitative study in the phenomenological tradition, using a semistructured in‐depth interview, of 20 young Bedouin men.ResultsFour themes were identified. “Power that I did not choose”: The patriarchal structure gives young men power and authority that they do not choose and cannot oppose. “The mirror of the older men is important”: Fulfilling the traditional Bedouin man's role is crucial for obtaining their elders' approval of their masculinity. “Responsibility”: The men have responsibility. “Masculine juggling”: The young men must negotiate their aspirations that conflict with society's expectations.ConclusionThe change that traditional Bedouin society is undergoing creates many challenges for young Bedouin men. These include having to be the authority figure in a patriarchal structure and the protector of the female family members, roles that often conflict with the men's personal desire for equality between the sexes and among family members.ImplicationsThe findings may be useful in planning support programs for educated young Bedouin men and may contribute to the understanding of the challenges facing men in Bedouin families.
A modified geometric technique to increase peri‐implant keratinized mucosa
Authors: Tang Chenxi , Zhang Peng , Zhang Xiaohan , Peng Lin 
Published: February 2024

AbstractObjectiveThe free gingival graft (FGG) has been identified as the most effective method for increasing keratinized mucosa width (KMW). However, the challenge emerges in cases of extensive keratinized mucosa deficiency, where efficient utilization of the patient's limited keratinized tissue to achieve optimal results is crucial. This article introduces a modified geometric technique to address this clinical issue.Clinical ConsiderationsUtilizing geometric principles, the modified technique involves dividing the rectangular graft into two triangular or trapezoidal sections, which are then reassembled to form an approximate diamond shape. Through strategic cut and splice, the graft is reshaped to suit the recipient site.ConclusionPreliminary observations in cases employing the modified geometric technique have increased the KMW around implants. This method enhances graft utilization and offers a viable clinical option for surgical plans aimed at widening keratinized mucosa in instances of large‐area KMW deficiency.Clinical SignificanceThis article proposed a modified method to increase KMW, which may be an optimal choice for patients with insufficient KMW in large area, avoiding the waste of limited graft, decreasing patient morbidity, and effectively widening keratinized mucosa.
Comparison of timing of relapse in dogs with nonassociative immune‐mediated hemolytic anemia, thrombocytopenia, or polyarthritis
Authors: Sparrow Richard , Swann James W. , Glanemann Barbara 
Published: February 2024

AbstractBackgroundRelapse is a clinical concern in dogs diagnosed with immune‐mediated hemolytic anemia (IMHA), thrombocytopenia (ITP), or polyarthritis (IMPA). The average time to relapse is unknown, and evidence that vaccination is associated with disease relapse is lacking.Hypothesis/ObjectivesCompare the incidence of relapse in groups of dogs with IMHA, ITP, or IMPA over a 24‐month period after diagnosis and compare proportions of dogs that received vaccines in those dogs that did and did not relapse.AnimalsOne hundred sixty client‐owned dogs (73 with IMHA, 55 with ITP, 32 with IMPA).MethodsMedical records of dogs were reviewed with the goal of following cases for a minimum of 2 years. Incidence of relapse was calculated for each disease, and relapse rates in dogs that were or were not vaccinated after diagnosis were compared.ResultsRelapse rates at 12 months differed significantly among disease groups (P = .02), with a higher rate for IMPA (35%) compared to IMHA (11%) or ITP (11%). Relapse rate at 24 months was 41% for IMPA, 18% for IMHA, and 23% for ITP. Ninety percent of IMPA relapses occurred in the first 12 months after diagnosis, compared with 56% for IMHA and 50% for ITP. Vaccine administration after diagnosis was not associated with relapse (P = .78).Conclusions and Clinical ImportanceRisk of disease relapse in IMPA is highest in the first year after diagnosis, with a higher relapse rate compared with IMHA and ITP. The role of vaccination in disease relapse remains unclear.
14C and 14C‐10Be terrestrial age dating system for meteorites—New data for four recently fallen meteorites
Authors: Tauseef Mohammad , Leya Ingo , Gattacceca Jérôme , Hofmann Beda , Szidat Sönke , Braucher Régis ,   ASTER Team
Published: February 2024

AbstractWe perform a systematic and detailed study of the 14C and 14C‐10Be dating systems for meteorite terrestrial ages. Physical model calculations indicate that neither the 14C production rates nor the 14C/10Be production rate ratios are constant enough to be reasonably approximated by average values. By using simple averages, one introduces a significant size‐dependent bias into the database for meteorite terrestrial ages. By combining modeled 14C production rates and 14C/10Be production rate ratios with (22Ne/21Ne)cos ratios and assuming ~80% ablation losses, relatively easy to use correlations of 14C production rates and 14C/10Be production rate ratios as a function of (22Ne/21Ne)cos are established. The new correlations enable the determination of terrestrial ages that are more accurate than ages based solely on average values for 14C and/or 14C/10Be. We validate the model predictions by measuring 14C activity concentrations, 14C/10Be production rate ratios, 21Necos concentrations, and (22Ne/21Ne)cos ratios in four recently fallen meteorites: Mt. Tazerzait, Boumdeid (2011), Bensour, and SaU 606. The experimental data confirmed the model predictions, although the available data are insufficient to be conclusive. More data from freshly fallen meteorites are needed for validating the model predictions for different chondrite sizes and chondrite types.
Multitier regulation of the
E. coli
extreme acid stress response by CsrA
Authors: Gorelik Mark G. , Yakhnin Helen , Pannuri Archana , Walker Alyssa C. , Pourciau Christine , Czyz Daniel , Romeo Tony , Babitzke Paul 
Published: February 2024

ABSTRACT

CsrA is an RNA-binding protein that regulates processes critical for growth and survival, including central carbon metabolism, motility, biofilm formation, stress responses, and expression of virulence factors in pathogens. Transcriptomics studies in
Escherichia coli
suggested that CsrA repressed genes involved in surviving extremely acidic conditions. Here, we examine the effects of disrupting CsrA-dependent regulation on the expression of genes and circuitry for acid stress survival and demonstrate CsrA-mediated repression at multiple levels. We show that this repression is critical for managing the trade-off between growth and survival; overexpression of acid stress genes caused by
csrA
disruption enhances survival under extreme acidity but is detrimental for growth under mildly acidic conditions.
In vitro
studies confirmed that CsrA binds specifically to mRNAs of structural and regulatory genes for acid stress survival, causing translational repression. We also found that translation of the top-tier acid stress regulator,
evgA
, is coupled to that of a small leader peptide,
evgL
, which is repressed by CsrA. Unlike dedicated acid stress response genes,
csrA
and its sRNA antagonists,
csrB
and
csrC,
did not exhibit a substantial response to acid shock. Furthermore, disruption of CsrA regulation of acid stress genes impacted host-microbe interactions in
Caenorhabditis elegans
, alleviating GABA deficiencies. This study expands the known regulon of CsrA to genes of the extreme acid stress response of
E. coli
and highlights a new facet of the global role played by CsrA in balancing the opposing physiological demands of stress resistance with the capacity for growth and modulating host interactions.

IMPORTANCE

To colonize/infect the mammalian intestinal tract, bacteria must survive exposure to the extreme acidity of the stomach.
E. coli
does this by expressing proteins that neutralize cytoplasmic acidity and cope with molecular damage caused by low pH. Because of the metabolic cost of these processes, genes for surviving acid stress are tightly regulated. Here, we show that CsrA negatively regulates the cascade of expression responsible for the acid stress response. Increased expression of acid response genes due to
csrA
disruption improved survival at extremely low pH but inhibited growth under mildly acidic conditions. Our findings define a new layer of regulation in the acid stress response of
E. coli
and a novel physiological function for CsrA.
Engaging health care professionals in quality improvement: A qualitative study exploring the synergies between projects of professionalisation and institutionalisation in quality improvement collaborative implementation in Denmark
Authors: Carstensen Kathrine , Goldman Joanne , Kjeldsen Anne Mette , Lou Stina , Nielsen Camilla Palmhøj 
Published: February 2024

Objective To examine the projects of professionalisation and institutionalisation forming health care professions’ engagement in quality improvement collaborative (QIC) implementation in Denmark, and to analyse the synergies and tensions between the two projects given the opportunities afforded by the QICs. Methods This was a cross-sectional interview study with professionals involved in the implementation of two national QICs in Denmark involving 23 individual interviews and focus group discussions with 75 people representing different professional groups. We conducted a reflexive thematic analysis of the data, drawing on institutional contributions to organisational studies of professions. Results Study participants engaged widely in QIC implementation. This engagement was formed by a constructive interplay between the professions’ projects of professionalisation and institutionalisation, with only few tensions identified. The project of professionalisation relates to a self-oriented agenda of contributing professional expertise and promoting professional recognition and development, while the project of institutionalisation focuses on improving health care processes and outcomes and advancing quality improvement. Both projects were largely similar across professional groups. The interplay between the two projects was enabled by the bottom-up approach to implementation, participation of QI specialists, and a clear focus on developing and delivering high-quality patient care. Conclusions Future strategies for QIC implementation should position QICs as a framework that promotes the integration of professions’ projects of professionalisation and institutionalisation to successfully engage professionals in the implementation process, and thereby optimise the effectiveness of QICs in health care.
MRMPro: a web-based tool to improve the speed of manual calibration for multiple reaction monitoring data analysis by mass spectrometry
Authors: Wang Ruimin , Jiang Hengxuan , Lu Miaoshan , Tong Junjie , An Shaowei , Wang Jinyin , Yu Changbin 
Published: February 2024

Abstract
Background
As a gold-standard quantitative technique based on mass spectrometry, multiple reaction monitoring (MRM) has been widely used in proteomics and metabolomics. In the analysis of MRM data, as no peak picking algorithm can achieve perfect accuracy, manual inspection is necessary to correct the errors. In large cohort analysis scenarios, the time required for manual inspection is often considerable. Apart from the commercial software that comes with mass spectrometers, the open-source and free software Skyline is the most popular software for quantitative omics. However, this software is not optimized for manual inspection of hundreds of samples, the interactive experience also needs to be improved.

Results
Here we introduce MRMPro, a web-based MRM data analysis platform for efficient manual inspection. MRMPro supports data analysis of MRM and schedule MRM data acquired by mass spectrometers of mainstream vendors. With the goal of improving the speed of manual inspection, we implemented a collaborative review system based on cloud architecture, allowing multiple users to review through browsers. To reduce bandwidth usage and improve data retrieval speed, we proposed a MRM data compression algorithm, which reduced data volume by more than 60% and 80% respectively compared to vendor and mzML format. To improve the efficiency of manual inspection, we proposed a retention time drift estimation algorithm based on similarity of chromatograms. The estimated retention time drifts were then used for peak alignment and automatic EIC grouping. Compared with Skyline, MRMPro has higher quantification accuracy and better manual inspection support.

Conclusions
In this study, we proposed MRMPro to improve the usability of manual calibration for MRM data analysis. MRMPro is free for non-commercial use. Researchers can access MRMPro through http://mrmpro.csibio.com/. All major mass spectrometry formats (wiff, raw, mzML, etc.) can be analyzed on the platform. The final identification results can be exported to a common.xlsx format for subsequent analysis.
Transcriptomic and proteomic strategies to reveal the mechanism of Gymnocypris przewalskii scale development
Authors: Xu Baoke , Cui Yanrong , A. Linlin , Zhang Haichen , Ma Qinghua , Wei Fulei , Liang Jian 
Published: February 2024

Abstract
Background
Fish scales are typical products of biomineralization and play an important role in the adaptation of fish to their environment. The Gymnocypris przewalskii scales are highly specialized, with scales embedded in only specific parts of the dermis, such as the areas around the anal fin and branchiostegite, making G. przewalskii an ideal material for biomineralization research. In this study, we aimed to unveil genes and pathways controlling scale formation through an integrated analysis of both transcriptome and proteome, of which G. przewalskii tissues of the dorsal skin (no scales) and the rump side skin (with scales) were sequenced. The sequencing results were further combined with cellular experiments to clarify the relationship between genes and signaling pathways.

Results
The results indicated the following: (1) a total of 4,904 differentially expressed genes were screened out, including 3,294 upregulated genes and 1,610 downregulated genes (with a filtering threshold of |log2Fold-Change|> 1 and p-adjust < 0.05). The identified differentially expressed genes contained family members such as FGF, EDAR, Wnt10, and bmp. (2) A total of 535 differentially expressed proteins (DEPs) were filtered out from the proteome, with 204 DEPs downregulated and 331 DEPs upregulated (with a filtering threshold of |Fold-Change|> 1.5 and p < 0.05). (3) Integrated analyses of transcriptome and proteome revealed that emefp1, col1a1, col6a2, col16a1, krt8, and krt18 were important genes contributing to scale development and that PI3K-AKT was the most important signaling pathway involved. (4) With the use of the constructed G. przewalskii fibroblast cell line, emefp1, col1a1, col6a2, col16a1, krt8, and krt18 were confirmed to be positively regulated by the PI3K-AKT signaling pathway.

Conclusion
This study provides experimental evidence for PI3K-AKT controlled scale development in G. przewalskii and would benefit further study on stress adaptation, scale biomineralization, and the development of skin appendages.