bluuebunny/crossref_metadata_2025_split huggingface dataset:
6. USING VIRTUAL REALITY TO SIMULATE AN OPERATING ROOM: SATISFACTION AND RESULTS FROM THE FIRST PILOT STUDY FOR NURSES
Authors: Zabaleta Jiménez J , Blasco A , Fernández-Monge A , Lizarbe J A , Mainer M , Esnal T , Baez J , Aldazabal J 
Published: February 2024

Abstract

Introduction
Virtual reality (VR) provides a firsthand active learning experience through varying degrees of immersion. The aim of this pilot study is to evaluate the use of VR as a potential tool for training operating room nurses to perform thoracic surgery procedures.

Methods
This is an open parallel-group randomized clinical trial. One group will receive basic training followed by an assessment module. The experimental group will receive the same basic training, followed by thoracic surgery training, and then an assessment module.

Results
Fifty-five nurses participated in the study (50 females), with a mean age of 41.6 years. The average score obtained by each participant in the evaluation mode was 477 points. The experimental group (513 points) achieved an overall higher score than the control group (445 points; p=0.04). Regarding age, women in the second quartile of age among the participants (35-41 years) achieved significantly better results than the rest (p=0.04). When evaluating results based on the practice moment, exercises performed in the last 10 minutes yielded better results than those performed in the first 10 minutes (1064 points versus 554 points; p &lt;0.001). The most frequent adverse effect reported was blurred vision. The overall satisfaction rating with the experience was 8.5 out of 10.

Conclusion
Virtual reality is a useful tool for training operating room nurses.
15. UTILITY OF CHATGPT IN THE APPLICATION OF THREE-DIMENSIONAL RECONSTRUCTION MODELS OF THE STOMACH IN REVISIONAL BARIATRIC SURGERY
Authors: Fernando Trebolle J , Tirado Anglés G , Valero Sabater M , Pérez Zapata A I , Menal Muñoz P , García García A , Blas Laína J L 
Published: February 2024

Abstract

Introduction
Radiological three-dimensional reconstruction from CT images is very useful in pre-surgical planning and in training for complex cases using the preoperative studies performed on the patient. In revisional bariatric surgery, there is an undiscovered field with potential applications to develop with 3D reconstruction models. Our goal is to propose the applications offered by ChatGPT with 3D reconstruction models of post-bariatric surgery anatomy, whether it be vertical gastrectomy or the gastric reservoir and gastrojejunal anastomosis in the case of bypass.

Methods
Three-dimensional image anatomical models of the stomach and post-bariatric surgery anatomy have been developed. CT images are obtained with the Siemens Somatom Perspective 64® device. The images are retrieved in DICOM format and processing to achieve three-dimensional reconstruction is done with the OsiriX® software, performing manual segmentation and an image density modification. ChatGPT analyzes these databases and provides us with the options that can be exploited.

Results
Project ideas based on ChatGPT: 1 - Surgical planning assistant. 2 - Virtual reality training simulator. 3 - Diagnostic assistant. 4 - Patient education tool. 5 - Research tool.

Conclusion
Obtaining three-dimensional reconstruction images of post-bariatric surgery anatomy allows us to develop models that, as guided by ChatGPT, can be applied in different aspects in revisional bariatric surgery.
16. DEVELOPMENT AND CHARACTERIZATION OF A NEW VASCULAR GRAFT: PRECLINICAL STUDY
Authors: Durán Rey D , Sánchez Rumbo C , Crisóstomo V , Brito Pereira R , Lancero Méndez S , Sánchez Margallo F M 
Published: February 2024

Abstract

Introduction
Blood vessel obstruction and lack of nutrients cause tissue damage. Although commercial vascular grafts are a common surgical treatment option, they are not without issues, particularly with stenosis. Tissue engineering provides a promising solution for designing vascular grafts that could replace or repair blood vessels. Polyvinylidene fluoride (PVDF) is a synthetic material with piezoelectric properties and good physical, biological, and mechanical characteristics, which makes it an interesting option for cardiovascular applications.

Methods
The electrospinning technique and post-treatment protocols were used to produce electrospun PVDF grafts.

Results
PVDF scaffolds were fabricated with randomly distributed fibers, which exhibited significant porosity and adequate pore size within the measured range. The PVDF grafts exhibited Young's modulus with mechanical properties typical of ductile materials. In terms of biological properties, they showed promising results.

Conclusion
The grafts showed optimal biological and mechanical characteristics for application in the experimental model and in vivo assay.
21. VARIATION OF MICROBIOTA DEPENDING ON THE LOCATION OF COLORECTAL CANCER
Authors: Picaporte Fuentes P , Dziakova J , de la Serna S , Jaimes E , García Galocha J L , Rivera D , Iniesta P , Torres A 
Published: February 2024

Abstract

Introduction
Colorectal cancer (CRC) growth may be determined by various factors, with gut microbiota being one of them. The aim of this study is to Identify the microbiota differences in tumoral tissue (TT), non-tumoral tissue (NTT) and feces depending on CRC localization.

Methods
Prospective analysis was conducted on patients with CRC who underwent colorectal surgery in 2021 and 2022. Three samples were collected from each patient: feces, tissue from tumor (TT), and tissue from healthy colon (NTT). The study employed 16S rRNA massive sequencing techniques, followed by bioinformatics analyses.

Results
A total of 23 patients, comprising 18 males and 5 females, were included in the study. Tumor distribution revealed 12 cases in the right colon, 7 in the left colon, and 4 in the rectum. Significant differences were observed in beta diversity between the microbiota of feces and both types of tissue (TT and NTT). However, no differences in beta diversity were noted between TT and NTT.
NTT exhibited a predominance of the phylum Actinobacteriota, while Bacteroidetes was the main phylum in feces. TT was characterized by a higher proportion of Fusobacteriota, with the genera Fusobacteria and Streptococcus being predominant compared to feces and the genus Fusobacteria compared to NTT.
When considering tumor location, notable differences emerged. There was greater microbiota diversity in the right colon compared to the left colon and rectum. Phyla Clostridiales, Bifidobacteriales, Acidaminococcales, and Vibrionales were more abundant in the right colon, while Staphylococcales were more prevalent in the left colon.

Conclusion
The observed variations in microbiota based on tissue type and location imply its potential involvement in colorectal cancer (CRC) pathogenesis.
23. SURGICAL MANAGEMENT OF A HIGHLY COMPLEX CASE OF BILIARY LEAK FOLLOWING HEPATOBILIARY SURGERY
Authors: Perra T , Porcu A 
Published: February 2024

Abstract

Introduction
Biliary leaks are a frequent complication of hepatobiliary surgery. We present a particularly complex case.

Clinical case
A 46-year-old man underwent S4-8 hepatic bisegmentectomy for hepatocellular carcinoma. Two months later he underwent percutaneous drainage of a liver collection and later endoscopic placement of plastic biliary stents. Due to the persistence of the leak, it was decided to intervene surgically by suturing the biliary leak, draining the main bile duct using a Kehr drain, and cholecystectomy, with apparent resolution.
Two months later, sudden dyspnea with yellow-green emission, persistent cough, and episodes of bile vomiting appeared. CT of the chest and abdomen confirmed the presence of a biliobronchial fistula and a subdiaphragmatic biloma connected to the hemidiaphragm and initial parenchymal irritation. Percutaneous drainage of the biliary collection and thoracotomy surgery (transdiaphragmatic repair of biliary fistula, atypical lung resection of the right lower lobe, pleural cleaning, and diaphragmatic plastic surgery) were performed.
One month later, with the subdiaphragmatic collection persisting, a Roux-en-Y biliojejunal anastomosis, transjejunal loop, and liver drainage (U tube) were performed, along with drainage of the interhepatodiaphragmatic collection.
Subsequently, one month later, due to the persistence of the bile leak with interhepatodiaphragmatic collection, another Roux-en-Y anastomosis of the parenchymal fistula in the residual cavity was performed.
After 14 days, the patient was discharged in good clinical condition, which lasted over time, without collections.

Discussion
This case illustrates the complexity that can be associated with a common complication of hepatobiliary surgery.
Filling in the Gaps: Updates on Doxycycline Prophylaxis for Bacterial Sexually Transmitted Infections
Authors: Hazra Aniruddha , McNulty Moira C , Pyra Maria , Pagkas-Bather Jade , Gutierrez Jose I , Pickett Jim , Stewart Jenell , Bolan Robert K , Molina Jean-Michel , Celum Connie , Luetkemeyer Anne F , Klausner Jeffrey D 
Published: February 2024

Abstract
Over the past 2 decades, cases of sexually transmitted infections (STIs) from syphilis, gonorrhea, and chlamydia have been rising in the United States, disproportionately among gay, bisexual, and other men who have sex with men, as well as racial and ethnic minorities of all genders. In this review, we address updates about the evidence on doxycycline postexposure prophylaxis for prevention of bacterial STIs, including efficacy, safety, antimicrobial resistance, acceptability, modeling population impact, and evolving guidelines for use. Equitable implementation of doxycycline postexposure prophylaxis will require evaluation of who is offered and initiates it, understanding patterns of use and longer term STI incidence and antimicrobial resistance, provider training, and tailored community education.
“Every Story Tells a Picture”: Suspended Time in Ali Smith’s Seasons Novels
Authors: Cowtan Grace 
Published: February 2024

Abstract
Through both the viewing and the reading of art, Ali Smith’s 2016–2020 tetralogy, Autumn, Winter, Spring, and Summer, posits an ethics of meaning-making that responds to the multiplicity of time-as-it-is-lived. This essay explores how Smith portrays the viewing of art as an experience of temporal disjuncture by which habitual modes of perception and cognition are renewed. Ordinarily produced through a sequential linking of cause and effect, meaning is formed instead through a postcritical engagement, which proposes an interactive mode of understanding that operates within a network of relations. Open to difference and to alteration, this mode of interpretation is, I suggest, demanded from Smith’s readers as, through ekphrasis (the verbal description of visual art), Smith suspends the temporal progression of narrative form.
Novel sequence type of carbapenem-resistant Acinetobacter pittii ST1451 with enhanced virulence isolated from septicaemic neonates in India
Authors: Roy Subhasree , Morita Daichi , Bhattacharya Sushmita , Dutta Shanta , Basu Sulagna 
Published: February 2024

Abstract

Background
The clinical relevance of Acinetobacter pittii is increasing, but reports of this organism causing neonatal sepsis are rare.

Objectives
To understand the mechanisms of resistance and virulence of A. pittii isolated from neonatal blood belonging to a novel sequence type.

Materials and methods
Antibiotic susceptibility, MLST, WGS, phylogenomic comparison with a global collection of carbapenemase-harbouring A. pittii were done. To study the pathogenic potential of novel A. pittii, in vitro and in vivo assays were carried out.

Results and discussion
Two novel multidrug-resistant A. pittii from neonatal blood belonging to a novel sequence type 1451 (ST1451) were isolated. WGS revealed that the isolates were almost similar (147 SNP distant) and harbouring two carbapenem resistance genes blaNDM-1 with upstream ISAba125 and downstream bleMBL along with blaOXA-58 with upstream ISAba3. Other resistance genes included blaADC-25, blaOXA-533, aph(3″)-Ib, aph(3′)-VIa, aph(6)-Id, aac(3)-IId, mph(E), msr(E), sul2 and tet(39), different efflux pump genes and amino acid substitutions within GyrA (Ser81Leu) and ParC (Ser84Leu; Glu88Ala) were detected among the isolates. The study genomes were closely related to four strains belonging to ST119. The isolates showed biofilm production, serum resistance, growth under iron limiting condition, surface-associated motility and adherence to host cell. Isolates induced cytokine production in the host cell and showed mice mortality.

Discussion and conclusions
This study is the first report of the presence of blaNDM-1 in A. pittii from India along with another carbapenemase blaOXA-58. Emergence of highly virulent, multidrug-resistant A. pittii with attributes similar to A. baumannii calls for surveillance to identify the novel strains and their pathogenic and resistance potential.
Comparison between multiple-trait and random regression models for genetic evaluation of weight traits in Australian meat sheep
Authors: Paneru Uddhav , Moghaddar Nasir , van der Werf Julius 
Published: February 2024

Abstract
Random regression (RR) models are recommended as an alternative to multiple-trait (MT) models for better capturing the variance–covariance structure over a trajectory and hence more accurate genetic evaluation of traits that are repeatedly measured and genetically change gradually over time. However, a limited number of studies have been done to empirically compare RR over a MT model to determine how much extra benefit could be achieved from one method over another. We compared the prediction accuracy of RR and MT models for growth traits of Australian meat sheep measured from 60 to 525 d, using 102,579 weight records from 24,872 animals. Variance components and estimated breeding values (EBVs) estimated at specific ages were compared and validated with forward prediction. The accuracy of EBVs obtained from the MT model was 0.58, 0.51, 0.54, and 0.56 for weaning, postweaning, yearling, and hogget weight stages, respectively. RR model produced accuracy estimates of 0.56, 0.51, 0.54, and 0.54 for equivalent weight stages. Regression of adjusted phenotype on EBVs was very similar between the MT and the RR models (P &gt; 0.05). Although the RR model did not significantly increase the accuracy of predicting future progeny performance, there are other benefits of the model such as no limit to the number of records per animal, estimation of EBVs for early and late growth, no need for age correction. Therefore, RR can be considered a more flexible method for the genetic evaluation of Australian sheep for early and late growth, and no need for age correction.
Analysis of Inpatient Burn Patients Treated After the February 2023 Kahramanmaras Turkiye Earthquake and Examination of the Burn Profile According to Injury Time
Authors: Basaran Abdulkadir , Özlü Özer , Gürbüz Kayhan , Daş Koray 
Published: February 2024

Abstract
On February 6, 2023, a 7.7 and about 9 h later, a 7.6 Richter’s scale earthquake struck south of Turkiye, affecting more than 9 million people in 11 cities. This study aims to assess injury mechanisms, patient characteristics, and the outcome related to the earthquakes and the newly emerging living conditions. This study was conducted between February 6, 2023 and May 6, 2023, in a referral burn center. Patients who experienced burns either during the earthquakes or within 3 months in the region were included. The patient characteristics, time and place of burn injury, etiology, latency time to hospital admittance, outcome, and mortality were evaluated retrospectively. A total of 63 patients were included in our study cohort with a mean age of 21.84 ± 20.3 years. The burned total body surface area was 15.56% ± 20.3%. The burns that occurred during the earthquakes were mainly minor scalds, but the median time to hospital admission was 55 h. The burns after the first week to the end of day 45 were mainly flame burns that occurred in tents. The following burns up to the end of the third month included flame burns resulting in high mortality, and electrical burns mainly occurred during the restoration of the destroyed environment. In major disasters like earthquakes, burns during catastrophes are inevitable. Accommodating survivors in fireproof tents will be essential for avoiding fatalities from fires. In the restoration period of the settlements, the workers should be adequately informed about precautions when dealing with electric transmission lines.
Celastrus orbiculatus extract reverses precancerous lesions of gastric cancer by inhibiting autophagy via regulating the PDCD4-ATG5 signaling pathway
Authors: Zhu Fangyuan , Zhang Xiaoze , Wen Junsong , Liu Yanqing , Zhu Yaodong 
Published: February 2024

Abstract

Objectives
Celastrus orbiculatus ethyl acetate extract (COE) is the main extract of the stem of the Chinese herbal C. orbiculatus, which has anti-tumor and anti-inflammatory biological effects. Our previous study showed that COE had a certain reversal effect on the precancerous lesions of gastric cancer (PLGC) in rats, but the exact mechanism of action remains elusive. We aimed to explore the therapeutic effects of COE on PLGC and the potential mechanisms.

Methods
The PLGC rat model was successfully constructed by N-methyl-N´-nitro-N-nitrosoguanidine (MNNG) multifactorial induction method. Then, COE was prepared to treat the PLGC rat model. Hematoxylin & eosin staining was used to observe gastric mucosal lesions in rats, AB-PAS and HID-AB staining were used to observe intestinal metaplasia. PDCD4-ATG5 signaling pathway was detected by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR) in vivo, and autophagy level was detected by IHC, transmission electron microscopy, and RT-PCR in vivo. Besides, the PLGC (MC) cell model was successfully constructed by treating GES-1 cells with MNNG. Then, the morphology, proliferation, and apoptosis of MC cells, and the role of the PDCD4-ATG5 signaling pathway and autophagy in MC cells were evaluated by COE and after the overexpression of PDCD4 treatment.

Key findings
COE significantly improved gastric mucosal injury and cellular heteromorphism and retarded the progression of PLGC in rats. Further studies indicated COE not only inhibited the level of autophagy but also interfered with the PDCD4-ATG5 signaling pathway in vivo. On the other hand, COE treatment could effectively reverse MC cell damage, inhibit MC cell proliferation, and promote MC cell apoptosis. Furthermore, COE also promoted PDCD4 and inhibited ATG5 expression in vitro, and the inhibitory effect of COE on ATG5-mediated autophagy was further enhanced after the overexpression of PDCD4.

Conclusions
The study revealed that COE could regulate the PDCD4-ATG5 signaling pathway to inhibit autophagy in gastric epithelial cells, which contributes to reversing the progression of PLGC.
Protective effects of piperlongumine against adjuvant-induced arthritis in rats through modulating OPG/RANKL/NF-κB signaling pathway
Authors: Wu Sheng-dong , Wu Xin-jie , Wang Tian-tian , Jiang Fei , Hu Ming-wang , Li Rong , Liu Ji , Cai Li 
Published: February 2024

Abstract

Objectives
We examined the antirheumatoid effects of piperlongumine (PLM) on rat adjuvant-induced arthritis (AIA) and explored the underlying mechanisms involved.

Methods
PLM (2.5, 5, and 10 mg/kg) was administered intraperitoneally to AIA rats to assess its effectiveness. Blood, thymus, spleen, ankle joint, and synovial tissue samples were gathered for subsequent analyses, like enzyme-linked immunosorbent assay, thymus/spleen index measurement, ankle joint pathological examination, immunohistochemistry assay, polymerase chain reaction, and western blot assay. Moreover, the involvement of osteoprotegerin (OPG)/receptor activators of nuclear factor κB ligand (RANKL)/nuclear factor-κB (NF-κB) signaling was investigated.

Key findings
PLM effectively relieved inflammation and joint destruction in AIA rats, as indicated by reductions in hind paw swelling, arthritis index, thymus/spleen index, ankle joint pathological damage, production of TNF-α, IL-1β, and IL-6 in both serum and synovium, and osteoclast formation. Also, PLM treatment raised OPG production, reduced RANKL expression, and elevated the OPG/RANKL ratio in synovial tissues. Furthermore, PLM prevented IκBα degradation and phosphorylation, resulting in a reduced expression of the nuclear NF-κB p65 protein in AIA rat synovial tissues.

Conclusions
PLM demonstrated strong antiarthritic effects in rats with AIA by influencing the OPG/RANKL/NF-κB signaling pathway, highlighting its potential clinical relevance in treating rheumatoid arthritis.
Ontology-based knowledge representation for traditional martial arts
Authors: Hou Yumeng , Kenderdine Sarah 
Published: February 2024

Abstract
Traditional martial arts are treasures of humanity’s knowledge and critical carriers of sociocultural memories throughout history. However, such treasured practices have encountered various challenges in knowledge transmission and now feature many entries on the UNESCO list of intangible cultural heritage. In tackling the urgency of knowledge preservation through digital means, this project employs an ontology-based approach to model the conceptual realm of traditional martial arts. Accordingly, it creates the Martial Art Ontology (MAon), a comprehensive domain ontology with an annotated data resource incorporating entities and relations from embodied, epistemic, and sociocultural facets. MAon underlines the significance of embodied qualities and addresses relevant dimensions, such as kinesthetics, techniques, mnemonics, and tactics, along with stylistic, interpretative, and ideological components. It features scholarly terminology developed through literature analysis, interviews with masters, and expert validations. The instantiation of MAon is realized through annotating three archetypal Southern Chinese styles, offering exhaustive descriptions concerning techniques, forms, principles, and form sets, amongst others. In summary, the reported approach encodes the manifold of martial arts into a structured vocabulary and an interlinked data resource, accessible to both human-reading and machine-operating applications. By applying it to manifest a range of knowledge concepts, we demonstrate the potential of ontology-based datafication to create coherent representations for intangible cultural entities and to enable an interoperable data infrastructure across multimodal cultural archives.