bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput screening assay for PARP-HPF1 interaction inhibitors to affect DNA damage repair
Authors: Dhakar Saurabh S. , Galera-Prat Albert , Lehtiö Lari 
Published: February 2024

AbstractADP-ribosyltransferases PARP1 and PARP2 play a major role in DNA repair mechanism by detecting the DNA damage and inducing poly-ADP-ribosylation dependent chromatin relaxation and recruitment of repair proteins. Catalytic PARP inhibitors are used as anticancer drugs especially in the case of tumors arising from sensitizing mutations. Recently, a study showed that Histone PARylation Factor (HPF1) forms a joint active site with PARP1/2. The interaction of HPF1 with PARP1/2 alters the modification site from Aspartate/Glutamate to Serine, which has been shown to be a key ADP-ribosylation event in the context of DNA damage. Therefore, disruption of PARP1/2-HPF1 interaction could be an alternative strategy for drug development to block the PARP1/2 activity. In this study, we describe a FRET based high-throughput screening assay to screen inhibitor libraries against PARP-HPF1 interaction. We optimized the conditions for FRET signal and verified the interaction by competing the FRET pair in multiple ways. The assay is robust and easy to automate. Validatory screening showed the robust performance of the assay, and we discovered two compounds Dimethylacrylshikonin and Alkannin, with µM inhibition potency against PARP1/2-HPF1 interaction. The assay will facilitate the discovery of inhibitors against HPF1-PARP1/2 complex and to develop potentially new effective anticancer agents.
Integrated alternative approaches to select feed-efficient rainbow trout families to enhance the plant protein utilization
Authors: Kajbaf Kimia , Overturf Ken , Kumar Vikas 
Published: February 2024

AbstractImproving feed utilization efficiency is a challenge in aquaculture. Therefore, we developed an indirect benchmark to use in selecting trout for improved efficiency of feed utilization on plant protein (soy)-based diets, with the long-term goal of reducing the cost of commercial trout production. We used a four-part integrative approach to identify feed efficient individuals among 1595 fish coming from 12 genetically selected families by establishing the phenotypic relationship between feed conversion ratio (FCR) and body weight variations using compensatory feeding regimes. Additionally, we examined the nutritional composition of fish filet for each efficiency phenotype during the compensatory regimen. Our findings showed that the fish with the lowest weight loss during a feed deprivation period and the highest weight gain during the refeeding period (FD−/RF +) demonstrated the lowest FCR (FCR = 0.99) and consisted of individuals from several lines. This finding confirms the possibility of improving feed efficiency in mixed lines. Although feeding period has an effect on nutritional composition of fillet, such selection criteria did not show an effect on groups. Overall, successful selection for the improvement of feed efficiency will have a broad application to commercial fish selective breeding programs, leading to increased aquaculture sustainability in the long run.
Prevalence and causes of blindness and distance visual impairment in Chinese adult population in 2022 during the COVID-19 pandemic: a cross-sectional study
Authors: Wang Hua , Xu Zhi , Chen Dandan , Li Huihui , Zhang Junyan , Liu Qinghuai , Shen Han 
Published: February 2024

AbstractThis cross-sectional study aims to investigate the prevalence and causes of visual impairment (VI) and blindness in Jiangsu Province, China in 2022 during the COVID-19 pandemic. Participants (n = 13,208, aged 18–93) underwent comprehensive ocular examinations. The prevalence and causes of binocular VI (presenting visual acuity [VA] ≥ 20/400 and < 20/63 in the better eye) and blindness (presenting VA < 20/400 in the better eye) were assessed according to the World Health Organization (WHO) criteria. The estimation of refractive error prevalence was conducted using the following classification: myopia ≤  − 0.50 diopters (D), high myopia ≤  − 6.00 D, hyperopia ≥ 0.50 D, and anisometropia ≥ 1.00 D. The overall prevalence of binocular VI and blindness was 21.04% (95% confidence interval [CI] 20.35–21.74%) and 0.47% (95% CI 0.37–0.60%). The highest prevalence of binocular VI was in the population aged 18–24 years old (46.29%, [95% CI 44.30–48.28%]), those with education at university and above (43.47%, [95% CI 41.93–45.02%]), students (54.96%, [95% CI 52.73–57.17%]). Uncorrected refractive error (URE) was the leading cause of presenting binocular VI (93.40%) and blindness (50.79%). The prevalence of myopia was 54.75% (95% CI 53.90–55.60%). Actions are needed to control URE and myopia within the adult Chinese population, with a particular emphasis on the younger, well-educated demographic.
Evaluation of morphological traits, biochemical parameters and seeding availability pattern among Citrus limon ‘Assam lemon’ accessions across Assam
Authors: Akhtar Suraiya , Ahmed Raja , Begum Khaleda , Das Ankur , Saikia Sarat , Laskar Rafiul Amin , Banu Sofia 
Published: February 2024

AbstractThe Assam lemon is a highly valued Citrus cultivar known for its unique aroma, flavor, and appearance. This study aimed to investigate the morphological, seeding pattern and biochemical variations within 132 populations of Assam lemon from across 22 districts of Assam along with the control samples, with the objective to offer comprehensive understanding that could facilitate the improvement of breeding programs and further improvement of this important cultivar. Clustering based on UPGMA algorithm for morphological and seeding pattern data were analysed at population level, revealed two major clusters, where all the populations of Upper Assam districts were in the same cluster with the original stock (control population). The populations from Tinsukia and Dhemaji districts displayed more close similarities with the control population in comparison to populations of Upper Assam districts. Another interesting observation was regarding flowering patterns, while populations from Upper Assam districts excluding Golaghat district displayed both bisexual and unisexual flowers with less concentration of unisexual flowers, other remaining districts had bisexual and unisexual flowers of almost equal concentration. Unisexual flowers contained only the male reproductive organs with 40 anthers, while bisexual flowers had 36 anthers. Seeding patterns were examined across the districts, and it was found that populations from Tinsukia, Dhemaji, Lakhimpur, Dibrugarh, Jorhat, and the control population exhibited seedless characteristic while populations from other selected districts displayed a combination of seedless and seeded traits. Interestingly, Golaghat district appears as the linking district and showed availability of both seeded and seedless Assam lemon fruit, connecting the regions of Barak valley, Central, Lower, North and Upper Assam. Biochemical analysis showed significant variations across districts, however, the populations from Dhemaji, Tinsukia, Lakhimpur, Dibrugarh, and Jorhat districts displayed similarity with the control population. The study also investigated variability in soil nutrient content revealing substantial variation among the populations studied. This comprehensive investigation provides valuable insights into the morphological, seeding pattern, and biochemical diversity within the Assam lemon cultivar. These findings can be instrumental in breeding programs to enhance the cultivar, particularly in producing high-quality seedless fruits to meet consumer demands.
Growth performance, meat quality, strength of jejunum and leg bones of both sexes Cherry Valley ducks fed with zeolite
Authors: Wlaźlak Sebastian , Biesek Jakub , Banaszak Mirosław 
Published: February 2024

AbstractZeolite, as a natural mineral, could be a good additive for ducks, in line with pro-environmental trends. The study aimed to evaluate zeolite additives in feed for broiler ducks of both sexes on production results, meat quality, and the strength of the jejunum, tibia, and femur. The experiment used 200 Cherry Valley ducks, divided into a control group of males (CM) and females (CF) and an experimental group of males (ZM) and females (ZF). In the control groups, a commercial diet was used. In the experimental groups, 1% zeolite was added. The ZM group demonstrated higher body weight and weight gain than the CM group. Zeolite reduced the feed conversion ratio. A higher liver weight was found in the experimental group (ZM). Notably, zeolite influenced the weight of male pectoral muscles. Higher water loss in the pectoral muscles and higher protein content in the leg muscles were found in the same group. Females had a higher weight of neck and wings with skin. Female pectoral muscles had lower protein and water content. Zeolite in feed at a 1% level for broiler ducks could be recommended as a natural additive that positively affects the ducks' production results concerning good quality meat.
Natural course of post-COVID symptoms in adults and children
Authors: Sugiyama Aya , Takafuta Toshiro , Sato Tomoki , Kitahara Yoshihiro , Yoshinaga Yayoi , Abe Kanon , Chanroth Chhoung , Ataa Akuffo Golda , Phyo Zayar , Kurisu Akemi , Ko Ko , Akita Tomoyuki , Kishita Eisaku , Kuwabara Masao , Tanaka Junko 
Published: February 2024

AbstractMore than 200 million COVID-19 survivors have lasting symptoms after recovering, but the duration and related risk factors remain uncertain. This study focused on all 6551 patients diagnosed with COVID-19 at a medical institution in Hiroshima from March 2020 to July 2022. In November 2022, a questionnaire survey was conducted regarding post-COVID symptoms and their duration. The prevalence and duration of post-COVID symptoms were illustrated using the Kaplan–Meier method. Risk factors for symptoms lasting over 3 months and interfering with daily life were assessed via multivariate logistic regression. A total of 2421 survivors responded: 1391 adults, 1030 children, median age 34 years (IQR 9–55), 51·2% male, 36·7% hospitalized, median time from infection to the survey was 295 days (IQR 201–538). Upon their initial recovery, the prevalence of post-COVID symptoms was 78·4% in adults and 34·6% in children. Three months later, the rates were 47·6% and 10·8%. After over one year, they were 31·0% and 6·8%. Regarding symptoms interfere with daily life, 304 people (12.6%) experienced symptoms lasting for over three months, with independent risk factors including age, being female, diabetes mellitus, infection during the Delta period, and current smoking. There was no significant association between vaccination history and post-COVID symptoms.
Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell
Authors: Ahn Jae Sung , Park Woongkyu , Jeong Do Hyun , Choi Soo Bong , Ahn Sun Hee 
Published: February 2024

AbstractThe emergence of infectious diseases worldwide necessitates rapid and precise diagnostics. Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profile. Our photothermal PCR method expedited DNA amplification while retaining its detection sensitivity. Combining photothermal quantitative PCR with real-time fluorometry and non-invasive temperature measurement, we could amplify the target DNA within just 25 min, with a minimum detectable DNA amount of 50 picograms. This innovation in photothermal qPCR, leveraging the photothermal properties of gold nanoshells, will pave the way for immediate point-of-care diagnostics of nucleic acid biomarkers.
Glycerol kinase enzyme is a prognostic predictor in esophageal carcinoma and is associated with immune cell infiltration
Authors: Ying Fei , Chen Xuyong , Lv Lihong 
Published: February 2024

AbstractThe influence of lipid metabolism on tumorigenesis and progression has garnered significant attention. However, the role of Glycerol Kinase (GK), a key enzyme in glycerol metabolism, in Esophageal Carcinoma (ESCA) remains unclear. To further elucidate the relationship between GK and ESCA, we investigated GK expression levels using database information. Controlled studies employing immunohistochemistry were conducted on clinical ESCA tumor samples and normal specimens, confirming GK’s elevated expression in ESCA. Analysis of The Cancer Genome Atlas (TCGA) data via Kaplan–Meier (KM) survival plots revealed that increased GK expression correlates with poorer ESCA patient outcomes, particularly in overall survival (OS) and disease-specific survival (DSS). Multiple regression analysis indicated that elevated GK expression is an independent risk factor affecting ESCA prognosis. Statistical analysis of prognostic data from clinical samples further corroborated this finding. Moreover, there appears to be a significant correlation between GK expression and immune infiltration, specifically involving certain T and B lymphocytes. In conclusion, elevated GK expression in ESCA is strongly linked to poor prognosis and increased immune cell infiltration, highlighting its potential as an independent prognostic biomarker and a viable therapeutic target.
Significant increase in graupel and lightning occurrence in a warmer climate simulated by prognostic graupel parameterization
Authors: Michibata Takuro 
Published: February 2024

AbstractThere is little consensus among global climate models (CGMs) regarding the response of lightning flash rates to past and future climate change, largely due to graupel not being included in models. Here a two-moment prognostic graupel scheme was incorporated into the MIROC6 GCM and applied in three experiments involving pre-industrial aerosol, present-day, and future warming simulations. The new microphysics scheme performed well in reproducing global distributions of graupel, convective available potential energy, and lightning flash rate against satellite retrievals and reanalysis datasets. The global mean lightning rate increased by 7.1% from the pre-industrial period to the present day, which was attributed to increased graupel occurrence. The impact of future warming on lightning activity was more evident, with the rate increasing by 18.4$$\%\,\textrm{K}^{-1}$$

%

K

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1

through synergistic contributions of destabilization and increased graupel. In the Arctic, the lightning rate depends strongly on the seasonality of graupel, emphasizing the need to incorporate graupel into GCMs for more accurate climate prediction.
RETRACTED ARTICLE: Digital transformation, green innovation, and carbon emission reduction performance of energy-intensive enterprises
Authors: Liu Xiao-meng , Zhang Ying-quan 
Published: February 2024

AbstractDigital transformation and green innovation are powerful initiatives to achieve carbon peaking, carbon neutrality targets and high-quality economic development. Using a sample of high energy-consuming listed enterprises from 2012 to 2021, a double fixed-effect model is constructed to verify the effect of green innovation on the carbon emission reduction performance of high energy-consuming enterprises, and digital transformation is used as a moderating variable to analyze the inner mechanism of green innovation affecting the carbon emission reduction performance of high energy-consuming enterprises under the effect of digital transformation. The empirical results show that green innovation can significantly improve the carbon emission reduction performance of energy-consuming enterprises, while digital transformation positively moderates the effect of green innovation on the carbon emission reduction performance of energy-consuming enterprises. When considering the industry heterogeneity, the moderation effect of digital transformation is significant in the chemical raw materials and chemical products manufacturing industry and the electricity and heat production and supply industry, but the petroleum processing and coking and nuclear fuel processing industry, the non-metallic mineral products industry, the ferrous metal smelting and rolling processing industry and the non-ferrous metal smelting and rolling processing industry are not yet significantly affected by green innovation and digital transformation. The findings of the study provide empirical evidence to promote the improvement of carbon emission reduction performance of energy-intensive enterprises in China and to achieve the "double carbon" target.
Novel molecular hepatocellular carcinoma subtypes and RiskScore utilizing apoptosis-related genes
Authors: Zhang Menggang , Zhang Shuijun , Guo Wenzhi , He Yuting 
Published: February 2024

AbstractHepatocellular carcinoma (HCC) is the third leading cause of global cancer-related deaths. Despite immunotherapy offering hope for patients with HCC, only some respond to it. However, it remains unclear how to pre-screen eligible patients. Our study aimed to address this issue. In this study, we identified 13 prognostic genes through univariate Cox regression analysis of 87 apoptosis-related genes. Subsequently, these 13 genes were analyzed using ConsensusClusterPlus, and patients were categorized into three molecular types: C1, C2, and C3. A prognostic model and RiskScore were constructed using Lasso regression analysis of 132 significant genes identified between C1 and C3. We utilized quantitative polymerase chain reaction to confirm the model’s transcript level in Huh7 and THLE2 cell lines. Both molecular subtypes and RiskScores effectively predicted patients benefiting from immunotherapy. Cox regression analysis revealed RiskScore as the most significant prognosis factor, suggesting its clinical application potential and providing a foundation for future experimental research.
Polypyrrole-wool composite with electrical heating properties fabricated via layer-by-layer method
Authors: Lee Suhyun 
Published: February 2024

AbstractThis study presents the development of conductive polymer-textile composites with outstanding electric heating properties achieved through the in-situ polymerization of polypyrrole on wool-felt fabrics, renowned for their superior thermal insulation. Employing successive layer-by-layer (LBL) cycles facilitated precise control over the uniform deposition of polypyrrole with the fabrics. The investigation focused on the interaction between wool fiber and polypyrrole, evaluating appearance, add-on, and electrical heating performance with varying LBL cycles. The polymerization process resulted in the formation of spherical polypyrrole particles on the wool-felt, with deposition increasing alongside LBL cycle numbers. Mechanical properties, including tensile strength and bending rigidity, exhibited enhancement with polypyrrole deposition, while strain reduction was noted, with minimal influence from LBL cycles. Electrical properties, particularly surface resistance, displayed a rapid decrease up to the second LBL cycle. Concerning electrical heating performance, the application of a 12 V voltage resulted in a linear increase in surface temperature with increasing LBL cycles, peaking at 15.5 ℃. Notably, this sustained electrical heating effect persisted even after voltage removal, attributed to the low thermal conductivity of wool fiber. Moreover, the polypyrrole conductive layer maintained exceptional conductivity following repeated abrasion and washing, credited to improved uniformity through LBL cycles. The synergy of wool's insulating properties and polypyrrole's conductivity, as confirmed in this study, presents the potential for a highly efficient heating fabric. These developed materials exhibited improved heating performance, energy conservation, and minimal change in mechanical properties, making them suitable for applications such as electrical heating smart clothing.
Dissecting the spatiotemporal diversity of adult neural stem cells
Authors: Mitic Nina , Neuschulz Anika , Spanjaard Bastiaan , Schneider Julia , Fresmann Nora , Novoselc Klara Tereza , Strunk Taraneh , Münster Lisa , Olivares-Chauvet Pedro , Ninkovic Jovica , Junker Jan Philipp 
Published: February 2024

AbstractAdult stem cells are important for tissue turnover and regeneration. However, in most adult systems it remains elusive how stem cells assume different functional states and support spatially patterned tissue architecture. Here, we dissected the diversity of neural stem cells in the adult zebrafish brain, an organ that is characterized by pronounced zonation and high regenerative capacity. We combined single-cell transcriptomics of dissected brain regions with massively parallel lineage tracing and in vivo RNA metabolic labeling to analyze the regulation of neural stem cells in space and time. We detected a large diversity of neural stem cells, with some subtypes being restricted to a single brain region, while others were found globally across the brain. Global stem cell states are linked to neurogenic differentiation, with different states being involved in proliferative and non-proliferative differentiation. Our work reveals principles of adult stem cell organization and establishes a resource for the functional manipulation of neural stem cell subtypes.
The Effect of Background Noise, Bilingualism, Socioeconomic Status, and Cognitive Functioning on Primary School Children's Narrative Listening Comprehension
Authors: Carlie Johanna , Sahlén Birgitta , Johansson Roger , Andersson Ketty , Whitling Susanna , Brännström Karl Jonas 
Published: February 2024

Purpose:
This study focuses on 7- to 9-year-old children attending primary school in Swedish areas of low socioeconomic status, where most children's school language is their second language. The aim was to better understand what factors influence these children's narrative listening comprehension both in an ideal listening condition (in quiet) and for the primary school classroom, a typical listening condition (with multitalker babble noise).

Method:
A total of 86 typically developing 7- to 9-year-olds performed a narrative listening comprehension test (Lyssna, Förstå och Minnas [LFM]; English translation: Listen, Comprehend, and Remember) in two listening conditions: quiet and multitalker babble noise. They also performed the crosslinguistic nonword repetition test and a digit span backwards (DSB) test. A predictive statistical model including these factors, the children's degree of school language exposure, parental education level, and age was derived.

Results:
Listening condition had the strongest predictive value for LFM performance, followed by school language exposure and nonword repetition accuracy. Parental education level was also a significant predictor. There was a significant three-way interaction effect between listening condition, age, and DSB performance.

Conclusions:
Multitalker babble noise has a negative effect on children's narrative listening comprehension. The effect of multitalker babble noise could be explained by age differences in the ability to allocate working memory capacity during the narrative listening comprehension task, suggesting that younger children may be more vulnerable for missing information when listening in background noise than their older peers.

Supplemental Material:

https://doi.org/10.23641/asha.25209248