bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strategi Komunikasi Pemasaran Sebagai Media Promosi Dalam Meningkatkan Penjualan Pada PT Tunas Jaya
Authors: Amelia Shy She , Iswadi  
Published: December 2023

Tujuan penelitian ini adalah untuk mengetahui fungsi dari strategi komunikasi pemasaran sebagai media promosi dalam meningkatkan penjualan pada PT Tunas Jaya. Metode yang digunakan dalam penelitian ini adalah metode penelitian kualitatif dengan jenis penelitian yang digunakan studi kasus. Adapun metode pengumpulan data yang digunakan ialah wawancara dan observasi. Berdasarkan hasil penelitian yang dilakukan, diketahui jika strategi pemasaran PT Tunas Jaya dalam meningkatkan penjualan ialah mengugunakan media promosi baik secara offline maupun online. Kedua metode ini saling melengkapi, dan membaurkan fungsi mereka masing-masing secara maksimal. Adapun fungsi dari penggunaan media promosi offline dari PT Tunas Jaya adalah sebagai penunjang dalam peningkatan kerja sama dengan perusahaan BUMN dan swasta. Sedangkan penggunaan media promosi online, PT Tunas Jaya menggunakan website dan media sosial, dan mengarahkan fungsinya untuk membangun kesadaran merek masyarakat terhadap PT Tunas Jaya sekaligus produk-produk yang ditawarkan. Kedua media promosi ini memiliki peranan komunikasi pemasaran yang berbeda, namun tujuan keduanya sama. Yaitu, untuk menjangkau khalayak yang lebih luas, dan mempertahankan interaksi dengan pelanggan PT Tunas Jaya, baik pelanggan yang sudah mengenak merek sejak awal, ataupun pelanggan baru.

Kata Kunci: Strategi, Komunikasi, Media Promosi
Hubungan Persepsi Dengan Sikap Petani Terhadap Varietas Unggul Baru Padi di Kabupaten Tanjung Jabung Timur Provinsi Jambi
Authors: Elwadinata Frenco , Wulandari Siti Abir , Bobihoe Julistia , Yardha Yardha , Kadarusman Kadarusman , Putra Candra Dwi , Meilin Araz 
Published: December 2023

Varietas unggul merupakan salah satu komponen utama teknologi yang terbukti mampu meningkatkan produktivitas padi dan pendapatan petani. Keputusan untuk melakukan penggantian varietas unggul baru (VUB) dapat dilakukan oleh petani, yang berperan sebagai pengambil keputusan utama dalam penerapan penggunaan VUB dan pengembangan VUB padi. Dalam proses ini, persepsi dan sikap petani terhadap inovasi baru memainkan peran yang sangat penting dan menentukan. Penelitian ini bertujuan untuk mendekripsikan persepsi dan sikap petani terhadap varietas unggul baru serta menganalisis hubungan antara persepsi dengan sikap petani terhadap varietas unggul baru padi di Kabupaten Tanjung Jabung Timur. Penelitian ini akan dilaksanakan di beberapa desa yang merupakan desa sentra tanaman padi di Kabupaten Tanjung Jabung Timur, dengan jumlah sampel sebanyak 124 orang dengan menggunakan metode simple random sampling. Dari hasil perhitungan dapat diketahui bahwa Ha : 𝑥2 ≠ 0, atau nilai sign 0.000 < 0,05 maka tolak Ho sehingga terdapat hubungan persepsi dan sikap petani terhadap varietas unggul baru padi di Kabupaten Tanjung Jabung Timur diterima.
Identifikasi Faktor yang Berkontribusi dalam Strategi Pemasaran Kopi di Kelompok Harapan Maju di Kintamani, Bangli
Authors: Widari Upadani I Gusti Ayu 
Published: December 2023

Kopi merupakan sumber pendapatan bagi petani, penghasil bahan baku industri, dan penciptaan lapangan kerja serta pembangunan daerah bagi negara Indonesia termasuk Bali. Tujuan dari penelitian ini adalah untuk mengidentifikasi faktor-faktor yang mempengaruhi strategi pemasaran untuk pengembangan usaha kopi di Kelompok Harapan Maju. Metode yang digunakan dalam penelitian ini adalah metode deskriptif dengan menggunakan angket dan observasi, wawancara, dan dokumentasi. Data yang ditemukan dianalisis menggunakan analisis deskriptif dan kuantitatif dengan skala Likert. Tiga puluh sampel dari Kelompok Harapan Maju digunakan sebagai responden yang memberikan pendapat mereka untuk kuesioner tentang faktor internal dan eksternal. Penelitian menemukan bahwa faktor peluang internal memberikan persentase indeks rata-rata total tertinggi 82% dan terendah diberikan oleh faktor kelemahan 75%. Untuk total indeks persentase rata-rata, faktor internal dan eksternal adalah 78% yang berarti Kelompok Harapan Maju perlu memilih strategi pemasaran yang tepat di masa depan
Head-centric computing for vestibular stimulation under head-free conditions
Authors: La Scaleia Barbara , Brunetti Claudia , Lacquaniti Francesco , Zago Myrka 
Published: December 2023

Background: The vestibular end organs (semicircular canals, saccule and utricle) monitor head orientation and motion. Vestibular stimulation by means of controlled translations, rotations or tilts of the head represents a routine manoeuvre to test the vestibular apparatus in a laboratory or clinical setting. In diagnostics, it is used to assess oculomotor postural or perceptual responses, whose abnormalities can reveal subclinical vestibular dysfunctions due to pathology, aging or drugs.Objective: The assessment of the vestibular function requires the alignment of the motion stimuli as close as possible with reference axes of the head, for instance the cardinal axes naso-occipital, interaural, cranio-caudal. This is often achieved by using a head restraint, such as a helmet or strap holding the head tightly in a predefined posture that guarantees the alignment described above. However, such restraints may be quite uncomfortable, especially for elderly or claustrophobic patients. Moreover, it might be desirable to test the vestibular function under the more natural conditions in which the head is free to move, as when subjects are tracking a visual target or they are standing erect on the moving platform. Here, we document algorithms that allow delivering motion stimuli aligned with head-fixed axes under head-free conditions.Methods: We implemented and validated these algorithms using a MOOG-6DOF motion platform in two different conditions. 1) The participant kept the head in a resting, fully unrestrained posture, while inter-aural, naso-occipital or cranio-caudal translations were applied. 2) The participant moved the head continuously while a naso-occipital translation was applied. Head and platform motion were monitored in real-time using Vicon.Results: The results for both conditions showed excellent agreement between the theoretical spatio-temporal profile of the motion stimuli and the corresponding profile of actual motion as measured in real-time.Conclusion: We propose our approach as a safe, non-intrusive method to test the vestibular system under the natural head-free conditions required by the experiential perspective of the patients.
Clinical utility of metagenomic next-generation sequencing in pathogen detection for lower respiratory tract infections and impact on clinical outcomes in southernmost China
Authors: Hao Jinxiang , Li Weili , Wang Yaoyao , Zhao Jiangman , Chen Yu 
Published: December 2023

BackgroundToday, metagenomic next-generation sequencing (mNGS) has emerged as a diagnostic tool for infections. However, since Hainan has a complicated pathogen spectrum, the diagnostic value and impact on patient outcomes of mNGS in Hainan are to be explored.MethodsFrom April 2020 to October 2021, 266 suspected lower respiratory tract infections (LRTIs) patients in Hainan were enrolled, and specimens were collected before antibiotic treatment. Bronchoalveolar lavage fluid (BALF) samples were subjected to mNGS and culture to compare the diagnostic performance. Other conventional microbiological tests (CMT) were also performed. Patients’ treatments and clinical outcomes were recorded, and the antibiotic resistance genes (ARGs) were detected via mNGS workflow.ResultsThe positive rate of mNGS outperformed that of culture (87.55% vs. 39.30%, p&lt;0.001) and CMT (87.12% vs. 52.65%, p&lt;0.001). Specifically, mNGS detected more P. aeruginosa (12.03% vs 9.02%, p&lt;0.05), H. influenzae (9.77% vs 2.26%, p&lt;0.001), Aspergillus fumigatus (3.00% vs 0.75%, p&lt;0.05), Candida albicans (26.32% vs 7.52%, p&lt;0.001) and uncommon pathogens. It also demonstrated great diagnostic advantages in Mycobacterium tuberculosis with 80% sensitivity and 97.4% specificity. Over half of the patients (147, 55.26%) had modified empirical treatment according to mNGS results and 89.12% of them responded well. For three deaths with modified treatment, multiple drug resistance was predicted by mNGS and confirmed by antibiotic susceptibility test.ConclusionsThe application of mNGS can benefit clinics in pathogen identification and antimicrobial treatment stewardship. Physicians should be alert to some emerging uncommon pathogens, including Chlamydia Psittaci, Nocardia otitidiscaviarum, and rare NTM.
Promoting systems thinking through perspective taking when using an online modeling tool
Authors: Jordan Rebecca C. , Gray Steven , Boyse-Peacor Alita , Sorensen Amanda E. , Frantz Cynthia McPherson , Jauernig Johanna , Brehm Paul , Shammin Md Rumi , Petersen John 
Published: December 2023

IntroductionDisagreements between people on different sides of popular issues in STEM are often rooted in differences in “mental models,” which include both rational and emotional cognitive associations about the issue; especially given these issues are systemic in nature.MethodsIn the research described here, we employ the fuzzy cognitive mapping software MentalModeler (developed by one of the authors)1 as a tool for articulating implicit and explicit assumptions about one’s knowledge of both the environmental and social science and values underpinning complex system related issues. More specifically, we test the assumption that this pedagogical approach will foster certain aspects of perspective taking that can be traced with cognitive development and systems thinking as students not only articulate their own understanding of an issue, but also articulate the view of others.Results and discussionResults are discussed with respect to systems thinking that is developed through this type of modeling.
Association of trajectory of body shape index with all-cause and cause-specific mortality: 18 years follow-up
Authors: Kazemian Elham , Mehran Ladan , Masoumi Safdar , Amouzegar Atieh , Azizi Fereidoun 
Published: December 2023

ObjectivesThe current study aimed to examine how the trajectory of a body shape index (ABSI) could predict mortality in a prospective cohort of 5587 participants.MethodsA Growth Mixture Model (GMM) was employed to identify ABSI and body shape trajectories spanning from 2000 to 2018. Multivariate Cox regression models with hazard ratio (HR) and 95% confidence intervals (CIs) were built to assess the association of death from all-cause and cardiovascular disease (CVD) with ABSI and body shape trajectories.ResultsWe found that individuals with a low ABSI–marked increase (Class II) and high ABSI–marked increase trajectory (Class III) had a higher risk of all-cause (adjusted HR for Class II, 1.37; 95%CI, 1.04-1.79; adjusted HR for Class III, 1.42; 95%CI, 1.05-1.91) and non- CVD mortality (adjusted HR for Class II, 1.38; 95%CI, 1.00-1.91; adjusted HR for Class III, 1.42; 95%CI, 1.00-2.05) as well as an increased risk of CVD (adjusted HR for Class II, 1.40; 95%CI, 1.14-1.71; adjusted HR for Class III, 1.42; 95%CI, 1.13-1.78) and coronary heart disease (CHD) (adjusted HR for Class II, 1.52; 95%CI, 1.18-1.96; adjusted HR for Class III, 1.47; 95%CI, 1.11-1.95. The trajectories of body shape phenotypes did not show any significant associations with mortality, CVD, or CHD events.ConclusionsABSI trajectories might be associated with subsequent risk of mortality and CVD events.
Estrobolome dysregulation is associated with altered immunometabolism in a mouse model of endometriosis
Authors: Alghetaa Hasan , Mohammed Amira , Singh Narendra P. , Bloomquist Ryan F. , Chatzistamou Ioulia , Nagarkatti Mitzi , Nagarkatti Prakash 
Published: December 2023

IntroductionEndometriosis is a painful disease that affects around 5% of women of reproductive age. In endometriosis, ectopic endometrial cells or seeded endometrial debris grow in abnormal locations including the peritoneal cavity. Common manifestations of endometriosis include dyspareunia, dysmenorrhea, chronic pelvic pain and often infertility and symptomatic relief or surgical removal are mainstays of treatment. Endometriosis both promotes and responds to estrogen imbalance, leading to intestinal bacterial estrobolome dysregulation and a subsequent induction of inflammation.MethodsIn the current study, we investigated the linkage between gut dysbiosis and immune metabolic response in endometriotic mice. Ovariectomized BALB/c mice received intraperitoneal transplantation of endometrial tissue from OVX donors (OVX+END). Control groups included naïve mice (Naïve), naïve mice that received endometrial transplants (Naive+END) and OVX mice that received the vehicle (OVX+VEH). Colonic content was collected 2 weeks post-transplantation for 16s rRNA pyrosequencing and peritoneal fluid was collected to determine the phenotype of inflammatory cells by flow cytometry. ResultsWe noted a significant increase in the number of peritoneal fluid cells, specifically, T cells, natural killer (NK) cells, and NKT cells in OVX+END mice. Phylogenetic taxonomy analysis showed significant dysbiosis in OVX+END mice, with an increase in abundance of Phylum Tenericutes, Class Mollicutes, Order Aneroplasmatales, and Genus Aneroplasma, and a decrease in Order Clostridiales, and Genus Dehalobacterium, when compared to OVX+VEH controls. The metabolomic profile showed an increase in some tricarboxylic acid cycle (TCA)-related metabolites accompanied by a reduction in short-chain fatty acids (SCFA) such as butyric acid in OVX+END mice. Additionally, the mitochondrial and ATP production of immune cells was enforced to a maximal rate in OVX+END mice when compared to OVX+VEH mice.ConclusionThe current study demonstrates that endometriosis alters the gut microbiota and associated immune metabolism.
Monitoring the reduction of UO3 thin film by hydrogen atoms using valence-level spectroscopy: correlating the U5f1 signal to surface hydroxyls
Authors: El Jamal G. , Gouder T. , Eloirdi R. , Jonsson M. , Idriss H. 
Published: December 2023

The reaction of a UO3 thin film with atomic hydrogen was studied by He(II) ultraviolet photoelectron spectroscopy (UPS) in the temperature range 190–300 K. UO3 reduction was instantaneously observed once it contacted H atoms at 10–7 torr. The reduction was manifested by the presence of U5f1 electrons in He(II) UPS at approximately 1.5 eV below the Fermi level. Based on the peak characteristics, the valence band shape (composed largely of O2p orbitals in addition to some contribution from U6d and U5f orbitals), and X-ray photoelectron spectroscopy (XPS) U4f lines, the reduction of U6+ in UO3 only results in the formation of U5+ cations and was largely limited to those on the surface. Associated with the reduction was the formation of surface hydroxyls (-OH species) due to the transfer of a proton of the H atom (H.) to surface oxygen ions, while the electron of H. is transferred to a U5f orbital. The pseudo-first-order rate constant of the initial rate of reduction at 10–7 torr and 190 K was found to be approximately 0.01 s–1. Qualitative analysis of the valence band before and after reduction indicates that O2p hybridization with U6d and U5f orbitals leads to well-distinguished features that are characteristic of UO3, U2O5, and UO2. These features, which were quantitatively reversed during the redox process, furthers the assessment of the stoichiometry of a given binary uranium oxide.
Exploring the involvement of the alternative complement pathway in non-infectious uveitis pathogenesis
Authors: Kulshrestha Prerna , Goel Pallavi , Murthy Somasheila , Tyagi Mudit , Basu Soumvaya , Gogri Pratik , Kaur Inderjeet 
Published: December 2023

PurposeNon-infectious uveitis is a complex disease characterized by intraocular inflammation of the uveal area and the leading cause of vision impairment and blindness in young people globally. However, what triggers inflammation and contributes to its recurrence remains unclear. The complement system has been linked to various immunological and inflammatory conditions. In the present study, we have systematically evaluated the role of the alternative complement pathway in the pathogenesis of non-infectious uveitis.MethodologyQuantitative PCR was done in the peripheral leukocytes to study the expression of genes and regulatory miRNA in both anterior and posterior uveitis (n=28 in each category). Multiplex ELISA was performed to measure alternative pathway complement components, such as C3b, factor B, and CFH, and aqueous humor of infectious and non-infectious uveitis patients and non-inflammatory controls (n=10 each). Western blotting was done to validate the ELISA findings in a subset of patients and controls.ResultsDownregulation of C3 and CFH mRNA in the peripheral blood was shown by quantitative PCR in the group of anterior uveiits (AU), while the opposite result was found in the group of posterior uveitis (PU). ELISA levels of C3b and CFH proteins were significantly higher in aqueous humor of infectious and non-infectious uveitis (*p = 0.03 and **p = 0.0007 respectively) as compared to the control group. Western blotting further validated (VitH) the activation of the complement cascade in the aqueous (AH) and vitreous humor of patients with non-infectious uveitis, with an increased level of C3b (n=6) and CFH (n=4) in aqueous humor. C3b level was significantly increased while CFH was reduced relative to controls in the vitreous humor (VitH) of posterior uveitis patients compared to controls (n=27 in each category). A C3b to CFH ratio was computed to assess the regulation of complement activation and this index was several folds higher in both anterior and posterior uveitis (n=10 each). The expression of miRNA-hsa-miR-146a and miRNA-hsa-miR-155-5p that regulates CFH was downregulated and nicely correlated with the increased complement proteins in both anterior and posterior uveitis (n=10 each).ConclusionOur results demonstrate a clear role of CFH and the activation of the alternative complement pathway in the pathogenesis of non-infectious uveitis; however, its therapeutic potential warrants further investigations.
Correlation of endoplasmic reticulum stress patterns with the immune microenvironment in hepatocellular carcinoma: a prognostic signature analysis
Authors: Zhan Ke , Yang Xin , Li Shuang , Bai Yang 
Published: December 2023

BackgroundsThe extended duration of endoplasmic reticulum stress (ERS) can impact the progression of hepatocellular carcinoma (HCC) and the efficacy of immunotherapies by interacting with immune cells that have infiltrated the tumor microenvironment (TME).Methods and resultsThe study utilized a training cohort of 364 HCC patients with complete information from The Cancer Genome Atlas Program (TCGA) database, and a validation cohort of 231 HCC patients from the International Cancer Genome Consortium (ICGC) database. The genes related to ERS exhibiting a strong correlation with overall survival (OS) were identified using univariate Cox regression analysis. A 13-gene predictive signature was then produced through the least absolute shrinkage and selection operator (LASSO) regression approach. The data revealed that the ERS-associated gene signature effectively stratified patients into high- or low-risk groups regarding OS in both the training and validation cohorts (P &lt; 0.0001 and P = 0.00029, respectively). Using the multivariate method, it is still an independent prognostic factor in both the training and validation cohorts (P &lt; 0.001 and P = 0.008, respectively). Moreover, several metabolic pathways were identified to be enriched among the 13 genes in the predictive signature. When the ERS-associated gene signature was combined with the tumor-node-metastasis (TNM) stage, the ERS nomogram performed better than either the gene signature or the TNM stage alone (C-index values: 0.731, 0.729, and 0.573, respectively). Further analysis revealed that patients in the high-risk group exhibited increased infiltration of immune cells. Additionally, GP6 was downregulated in HCC tissues among these signature genes (P &lt; 0.05), which was related to poor OS.ConclusionsThe data suggest that this novel ERS-associated gene signature could contribute to personalized cancer management for HCC. Moreover, targeting GP6 inhibition might be a potential method for HCC therapy.
Case report: Differential diagnosis of highly amplified anti-CD5 CAR T cells and relapsed lymphoma cells in a patient with refractory ALK positive anaplastic large cell lymphoma
Authors: Mu Wei , Zhang Meilan , Hu Guang , Han Yunfeng , Mao Xia , Chen Caixia , Shen Kefeng , Dai Zhenyu , Zhu Xiaojian , Zhou Xiaoxi , Huang Liang , Ao Qilin , Xiao Min 
Published: December 2023

BackgroundAnaplastic Large Cell Lymphoma (ALCL) is one of the most common subtypes of T-cell lymphoma. Among these, refractory and relapsed (r/r) ALK positive ALCL lacks effective therapies. The chimeric antigen receptor-modified T (CAR-T) cell therapy holds great promise as a therapeutic strategy for this disease. However, it is not known yet whether anti-CD5 CAR-T cells are sufficient for the definitive treatment of relapsed ALK+ ALCL, nor the role of accurate laboratory-based diagnoses during CAR-T treatment.Case presentationThe adolescent patient received autologous T cells containing sequences encoding VH domains specific to CD5. Following the infusion, there was an increase in both the copy number and proportion of CAR-T cells in peripheral blood. IL-6 and ferritin levels in the patient exhibited significant fluctuations, with increases of 13 and 70 folds respectively, compared to baseline after the treatment. Additionally, adverse effects were observed, including grade 4 rash, grade 1 headache, nausea, and neck-pain. Surprisingly, a relapsed disease phenotype was identified based on the results of PET/CT and histopathological analysis of the inguinal lymph node biopsy. After conducting a thorough diagnostic assessment, which included flow cytometry, next-generation sequencing (NGS), examination of immune-related gene rearrangements, and analysis of the immune repertoire of T-cell receptors (TCR), we conclusively determined that the hyperplastic T cells identified in the lymph node were the result of an expansion of CAR-T cells. Ultimately, the patient has attained complete remission (CR) and has sustained a disease-free survival state for 815 days as of the cutoff date on August 30, 2023.ConclusionTaken together, the results demonstrate that anti-CD5 CAR-T cells can induce a clinical response in r/r ALK+ ALCL patient. Furthermore, this case underscores the importance of utilizing advanced technologies with high sensitivity and accuracy for biological detection in clinical laboratory diagnosis and prognosis in CAR-T cell treatment.Trial registration numberNCT04767308.