bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of traditional or heat‐not‐burn cigarette smoking on circulating miRNAs in healthy subjects
Authors: Picchio Vittorio , Ferrero Giulio , Cozzolino Claudia , Pardini Barbara , Floris Erica , Tarallo Sonia , Dhori Xhulio , Nocella Cristina , Loffredo Lorenzo , Biondi‐Zoccai Giuseppe , Carnevale Roberto , Frati Giacomo , Chimenti Isotta , Pagano Francesca 
Published: December 2023

AbstractBackgroundTraditional combustion cigarette (TCC) smoking is an established risk factor for several types of cancer and cardiovascular diseases. Circulating microRNAs (miRNAs) represent key molecules mediating pathogenetic mechanisms, and potential biomarkers for personalized risk assessment. TCC smoking globally changes the profile of circulating miRNAs. The use of heat‐not‐burn cigarettes (HNBCs) as alternative smoking devices is rising exponentially worldwide, and the circulating miRNA profile of chronic HNBC smokers is unknown. We aimed at defining the circulating miRNA profile of chronic exclusive HNBC smokers, and identifying potentially pathogenetic signatures.MethodsSerum samples were obtained from 60 healthy young subjects, stratified in chronic HNBC smokers, TCC smokers and nonsmokers (20 subjects each). Three pooled samples per group were used for small RNA sequencing, and the fourth subgroup constituted the validation set.ResultsDifferential expression analysis revealed 108 differentially expressed miRNAs; 72 exclusively in TCC, 10 exclusively in HNBC and 26 in both smoker groups. KEGG pathway analysis on target genes of the commonly modulated miRNAs returned cancer and cardiovascular disease associated pathways. Stringent abundance and fold‐change criteria nailed down our functional bioinformatic analyses to a network where miR‐25‐3p and miR‐221‐3p are main hubs.ConclusionOur results define for the first time the miRNA profile in the serum of exclusive chronic HNBC smokers and suggest a significant impact of HNBCs on circulating miRNAs.
Clinimetrics of the Lanarkshire Oximetry Index for patients with leg ulcers: A systematic review and meta‐analysis
Authors: Wynn Matthew , Stephens Melanie , Pradeep Sheba , Clarke‐Cornwell Alexandra M. , Bianchi Janice 
Published: December 2023

AbstractAnkle Brachial Pressure Index (ABPI) measurement has long been considered the gold standard of vascular assessment for people with lower limb ulceration. Despite this, only around 15% of patients in the United Kingdom who require an ABPI measurement undergo the assessment. The Lanarkshire Oximetry Index (LOI) is a cheaper and arguably more accessible approach to vascular assessment and was initially proposed as an alternative to the ABPI in 2000. No synthesis of evidence related to the LOI has been performed since its introduction into the literature. Primary studies were sought to determine the clinimetric properties of the LOI and its level of agreement with ABPI assessments. Systematic searches of MEDLINE, CINAHL, Cochrane Central Register of Controlled Trials, BNI, ProQuest Health and Medicine, Science Direct, Google Scholar and the British Library (online search) were conducted. Reference lists of identified studies were also reviewed to identify additional studies. Three primary studies met the inclusion criteria, reporting data from 307 patients and 584 limbs assessed using both the LOI and ABPI. All three studies reported fair to moderate kappa values for interrater reliability (κ = 0.290–0.747) and statistically significant positive correlation coefficients (r = 0.37, p < 0.001 in two studies) between the LOI and ABPI. The combined data from the three studies indicated a sensitivity of 52% (41.78–62.1, 95% confidence interval [CI]) and specificity of 96.08% (93.4–97.9, 95% CI) for the LOI using the ABPI as a reference. Additional data are required to indicate the safety of the LOI in practice. Data are also required to determine if the LOI is more acceptable to clinicians compared to the ABPI and whether there are any barriers/enablers to its implementation in practice. Given the relatively low specificity of the LOI, it may be beneficial to combine measurement of the LOI with a subjective clinical risk assessment tool to improve the sensitivity of this alternative approach to vascular assessment.
A review of supply chain transparency research: Antecedents, technologies, types, and outcomes
Authors: Budler Marko , Quiroga Bernardo F. , Trkman Peter 
Published: December 2023

AbstractThe growing popularity of “Supply Chain Transparency” (SCT) as an idea has motivated its use as a “buzzword,” leading to a lack of terminological clarity. Terms like visibility, transparency, and traceability are used colloquially or as synonyms for one another, yet often without a proper conceptual basis. We argue that the absence of a clear understanding of SCT limits the ability of scholars and practitioners to fully understand the role played by transparency in supply chains and to study its antecedents, technologies, types, and outcomes. Therefore, transparency research is at the point where a scoping review of literature on SCT is particularly useful. Our review first provides a formal conceptualization of SCT, allowing us to identify how transparency has been understood, the building blocks of SCT, and what distinguishes SCT from other concepts. In the review, we initially identify relevant concepts underlying SCT with a view to establish a unifying and nuanced framework for SCT. Second, using that unified conceptualization as a basis, primary outcomes such as the benefits and risks of SCT are determined. Third, we systematically detect and classify future research opportunities arising from the building blocks of SCT.
Detection of early bruises on apples using near‐infrared camera imaging technology combined with adaptive threshold segmentation algorithm
Authors: Tian Mimi , Zhang Jiachuang , Yang Zengrong , Li Mei , Li Junhui , Zhao Longlian 
Published: December 2023

AbstractApples are highly susceptible to bruising during picking, packing, and transportation, and early detection of apple bruises allows timely screening and treatment to reduce food safety risks. However, early bruises on apples, especially bruises of 30 min or less, with similar appearance to sound tissue, making manual sorting difficult. To address this problem, a near‐infrared (NIR) camera imaging technology combined with adaptive threshold segmentation algorithm for early apple bruise detection is proposed in this article. First, a NIR camera imaging system is built to acquire full‐band images of fruit face, fruit stem face and calyx face of sound apples and apples with single bruise and multiple bruises at 30 min of occurrence. Then, an adaptive threshold segmentation algorithm is proposed and an image processing algorithm, which includes high‐pass filtering, feature extraction, adaptive threshold segmentation and Hough circle detection, is developed and applied to full‐band images in response to the interference of apple stem and calyx on early bruising detection in this article. The results show that the proposed algorithm has an accuracy of 95.56% for the detection of sound apple samples and an F1‐score of 94.70% for the detection of early bruised samples, which is better than the commonly used image segmentation algorithms such as Otsu and fixed thresholding. This study shows that the combination of NIR camera imaging and the adaptive threshold segmentation algorithm proposed in this article can effectively detect early bruises on apples, providing a new idea for rapid and nondestructive detection of agricultural product quality.Practical applicationsDetecting early bruises on apples can achieve effective monitoring and control of apple supply chain, and guarantee the quality and safety of agricultural products. Most studies are based on conventional machine vision with RGB camera or hyperspectral imaging technology, while conventional machine vision based on RGB camera is difficult to detect apple early bruises that are not visible to the naked eye, and hyperspectral imaging has a large amount of data and redundant image data among wavelengths. Compared with previous studies, apple early bruise detection method proposed in this article combining NIR camera imaging technology and adaptive threshold segmentation algorithm can avoid the interference of bright spots, fruit stem and calyx on bruise segmentation while capturing a single channel image, providing a new method for accurate and rapid detection of early bruises on apples.
Discrimination of artificial strawberry aroma by electronic nose based on nanocomposites
Authors: Feltes Giovana , Ballen Sandra C. , Soares Andrey Coatrini , Soares Juliana Coatrini , Paroul Natalia , Steffens Juliana , Steffens Clarice 
Published: December 2023

AbstractAn electronic nose containing an array of gas sensors with interdigitated electrodes coated with nanocomposites based on polyaniline (Pani) combined with carboxylated multiwalled carbon nanotubes doped with different acids was used to discriminate artificial strawberry aromas. Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to investigate if the electronic nose was able to distinguish between different strawberry aromas. In addition, a nonlinear projection of the interactive document map technique (IDMAP) was applied to evaluate the distinction ability of the gas sensor array. The sensing layers showed tubular characteristics for Pani/MWCNT_COOH morphology and the group's representative doped state. The main and most common compound identified by gas chromatography in strawberry aromas was ethyl butyrate. The gas sensors showed high sensitivity (>6.55 mV/ppm), low limit of detection (LD <0.31 ppm), limit of quantification (LQ <5.43 ppm), short response time (<63 s) and recovery time (<10.15 s) for different artificial strawberry aromas. The results demonstrate that the electronic nose could differentiate among the artificial strawberry aromas. The volatile profile of strawberry aromas was distinguished into distinct groups using both LDA and PCA analysis. The use of IDMAP permitted the identification of the major contributors to the distinguishing ability of gas sensors with a high silhouette coefficient (0.91). The sensor array was efficient at aroma discrimination, demonstrating excellent potential for food analysis in industrial applications.Practical applicationsThe distinction between natural and artificial flavors has significantly shrunk as volatile chemical technology has advanced. Historically, it was difficult to industrially replicate natural aromas. Identification and analysis of aromas are challenging tasks. Targeted aroma compounds are frequently present in low concentrations (parts per million), which makes it very challenging to identify them. The electronic nose system is a promising new analytical method that can quickly and accurately, without pre‐treating the sample, detect and identify the full information of aroma compounds in real time. It is generally accepted as a nondestructive, affordable, and portable method with high feasibility, speed, and ease of use. For qualitative differentiation and distinguishing, PCA, LDA, and IDMAP methods were also used.
Chromosomal‐level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics
Authors: Jiang Jutang , Zhang Zeyu , Bai Yucong , Wang Xiaojing , Dou Yuping , Geng Ruiman , Wu Chongyang , Zhang Hangxiao , Lu Cunfu , Gu Lianfeng , Gao Jian 
Published: December 2023

ABSTRACTDendrocalamus brandisii (Munro) Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots. Its rapid growth and use as high‐quality material make this bamboo species highly valued for both food processing and wood applications. However, genome information for D. brandisii is lacking, primarily due to its polyploidy and large genome size. Here, we assembled a high‐quality genome for hexaploid D. brandisii, which comprises 70 chromosomes with a total size of 2,756 Mb, using long‐read HiFi sequencing. Furthermore, we accurately separated the genome into its three constituent subgenomes. We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly, revealing differential gene expression and post‐transcriptional regulation. By integrating metabolome analysis, we unveiled that well‐balanced lignin formation, as well as abundant flavonoid and fructose contents, contribute to the superior quality of D. brandisii shoots. Integrating genomic, transcriptomic, and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene‐editing techniques. This study should facilitate research on D. brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.
“Thin markets”: Recruitment and retention of disability staff to support effective post‐parental care planning in rural Australia
Authors: Wark Stuart , Bryant Lia , Morales‐Boyce Tyson 
Published: December 2023

AbstractThe life expectancy of persons with intellectual disability is increasing, and this is often occurring concurrently with the aging of their long‐term parental carers. Research in both Australia and around the world indicates that proactive post‐parental care planning is not widely implemented, and transitions primarily happen suddenly following a personal crisis for the primary caregiver. Little focus in Australia has been placed on identifying the barriers that inhibit post‐parental care planning in rural areas, specifically in the context of the newly implemented National Disability Insurance Scheme. This paper examines the factors that limit disability services and their staff in supporting successful post‐parental care planning for individuals with intellectual disability and their aging carers in rural South Australia. Small focus groups were conducted with three groups of rural disability support workers using online technologies to assist with the participants' geographic disparity. A semi‐structured interview guide was developed prior to commencement and was used to initiate discussions on key points. A thematic analysis methodological approach was used for data analysis. There were three themes identified through the analysis; Recruitment; Provision of Care; and Retention. The key findings relating to these themes are presented and supported with exemplar quotes. This research proposes three recommendations for policy or practice change: developing a national advertising campaign for new disability staff that positively emphasizes the high‐level skillset need for proactive planning; supporting rural disability providers to collaborate to establish shared teams of staff with expertise in post‐parental care planning; and, using incentive payments to retain staff with these invaluable skillsets in the disability sector.
A quantitative information measure applied to texture perception attributes during mastication
Authors: Sturtewagen Luka , van Mil Harald , de Lavergne Marine Devezeaux , Stieger Markus , van der Linden Erik , Odijk Theo 
Published: December 2023

AbstractWe have calculated an entropy or information measure of previously reported experimentally determined temporal dominance of sensations (TDS) data of texture attributes for two sets of emulsion filled gels throughout the mastication cycle. The samples were emulsion filled gels and two‐layered emulsion filled gels. We find that the entropy measure follows an average curve, which is different for each set. The specifics of the entropy curve may serve as a fingerprint for the perception of a specific food sample.
Clinical and genomic features of Listeria monocytogenes‐associated mesenteric lymphadenitis in a cat
Authors: Garcia‐de la Virgen Miguel , López‐Almela Inmaculada , Moura Alexandra , Vázquez Sergio , Perez‐Montagud Sara , Leclercq Alexandre , Lecuit Marc , Quereda Juan J 
Published: December 2023

AbstractBackgroundListeriosis is a severe foodborne infection caused by Listeria monocytogenes, an important foodborne pathogen of animals and humans. Listeriosis is a rare disease in cats.ObjectiveTo describe the clinical, diagnostic imaging, histological, and microbiological features of L. monocytogenes‐associated mesenteric lymphadenitis in a cat.AnimalsListeria monocytogenes‐associated mesenteric lymphadenitis was confirmed in a cat by histology and microbiology.ResultsTwo distinct isolates of L. monocytogenes were cultured from the affected mesenteric lymph node and whole genome sequencing was performed.Conclusion and Clinical ImportanceThis report should alert veterinary clinicians and microbiologists to the syndrome, which may have implications for health and food safety in animals and humans.
Cell‐type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm
Authors: Zhang Shuang , Ghatak Arindam , Mohammadi Bazargani Mitra , Kramml Hannes , Zang Fujuan , Gao Shuang , Ramšak Živa , Gruden Kristina , Varshney Rajeev K. , Jiang Dong , Chaturvedi Palak , Weckwerth Wolfram 
Published: December 2023

SummaryThe nutritional value of wheat grains, particularly their protein and metabolite composition, is a result of the grain‐filling process, especially in the endosperm. Here, we employ laser microdissection (LMD) combined with shotgun proteomics and metabolomics to generate a cell type‐specific proteome and metabolome inventory of developing wheat endosperm at the early (15 DAA) and late (26 DAA) grain‐filling stages. We identified 1803 proteins and 41 metabolites from four different cell types (aleurone (AL), sub‐aleurone (SA), starchy endosperm (SE) and endosperm transfer cells (ETCs). Differentially expressed proteins were detected, 67 in the AL, 31 in the SA, 27 in the SE and 50 in the ETCs between these two‐time points. Cell‐type accumulation of specific SUT and GLUT transporters, sucrose converting and starch biosynthesis enzymes correlate well with the respective sugar metabolites, suggesting sugar upload and starch accumulation via nucellar projection and ETC at 15 DAA in contrast to the later stage at 26 DAA. Changes in various protein levels between AL, SA and ETC support this metabolic switch from 15 to 26 DAA. The distinct spatial and temporal abundances of proteins and metabolites revealed a contrasting activity of nitrogen assimilation pathways, e.g. for GOGAT, GDH and glutamic acid, in the different cell types from 15 to 26 DAA, which can be correlated with specific protein accumulation in the endosperm. The integration of cell‐type specific proteome and metabolome data revealed a complex metabolic interplay of the different cell types and a functional switch during grain development and grain‐filling processes.
An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada
Authors: Gagnon Marie‐Claude , Duceppe Marc‐Olivier , Colville Adam , Pope Louise , Côté Marie‐José , Ogunremi Dele 
Published: December 2023

SummaryGlyphosate‐resistant wheat plants were discovered in southern Alberta in 2017, representing an unauthorized GM release in Canada. The Canadian Food Inspection Agency undertook a series of experiments to characterize and identify this unknown GM wheat, as well as to develop and validate construct‐specific and event‐specific qPCR assays. Results of PCR‐based assays and Sanger sequencing indicated the presence of CaMV 35S promoter (p35S), Rice Actin 1 intron (RactInt1), CP4‐EPSPS gene and nopaline synthase terminator (tNOS) elements in the unknown GM wheat. Genome walking and bead capture strategies, combined with high‐throughput sequencing, were used to identify the 5′ and 3′ wheat junctions and the subsequent mapping of the insert to chromosome 3B of the wheat genome. A probable transformation vector, pMON25497, was recognized, and further testing identified the unknown GM wheat as MON71200 event, one of two events obtained with the pMON25497 vector. The two construct‐specific assays targeted the junctions of the RactInt1 and the CP4‐EPSPS elements and the CP4‐EPSPS and tNOS elements, while the event‐specific assay was located at the 3′ junction into the wheat genome. Both construct‐specific and event‐specific assays had limits of detection of 0.10% of MON71200 in a seed pool. As expected, the two construct‐specific assays cross‐reacted with other wheat and corn events containing the same elements in the same order. No cross‐reactivity was observed for the event‐specific assay. The integrated strategy employed in this study can serve as a model for other cases when facing similar challenges involving unknown GM events.
Prevalence and diagnostic significance of non‐invasive follicular thyroid neoplasm with papillary‐like nuclear features in Japan—A multi‐institutional study
Authors: Hirokawa Mitsuyoshi , Ito Masahiro , Motoi Noriko , Chiba Tomohiro , Imamura Yoshiaki , Yasuoka Hironao , Hino Rumi , Higuchi Miyoko , Miyauchi Akira , Akamizu Takashi 
Published: December 2023

AbstractThis multi‐institutional study investigated non‐invasive follicular thyroid neoplasm with papillary‐like nuclear features (NIFTP) frequency and its diagnostic significance in Japan. We reviewed 4008 thyroid nodules resected in six institutions before NIFTP was proposed. Overall, 26 cases diagnosed as non‐invasive encapsulated follicular variant of papillary thyroid carcinoma (PTC) and 145 cases of follicular thyroid adenoma (FTA) were included. Of these nodules, 80.8% and 31.0%, respectively, were NIFTPs. In five institutions, NIFTPs were more commonly found in FTA than in PTC nodules. When NIFTP was included with PTC, the overall prevalence was 2.3%, with rates in five institutions below 5.0% (0.8%–4.4%). One NIFTP case with nuclear score 3 revealed nodal metastasis 2.5 years post‐resection, and the carcinoma cells were immunohistochemically positive for BRAF. FTAs or NIFTPs with nuclear score 2 did not metastasize. NIFTP was more common among FTA than among PTC nodules, possibly due to underdiagnosis of PTC on nuclear findings. Considering the clinical findings, molecular pathogenesis, and therapeutic strategy in Japan, NIFTP with nuclear score 2 is not different from FTA, and use of this entity terminology is not meaningful. In contrast, NIFTP with nuclear score 3 has potential for metastasis and BRAFV600E mutation. Therefore, in NIFTP cases, nuclear scores 2 and 3 should be separately reported.