bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical simulation of gas-solid flow in a cyclone separator with additional inlet
Authors: Noori Shahla , Saidi Maysam 
Published: January 2024

Abstract
In this study, an additional inlet was added to the gas-solid cyclone separator to enhance the separation efficiency. Four different heights were tested, including 0.95D, 1.4D, 1.5D, and 1.95D (D is the diameter of the cylindrical section). The investigation involved two inlet flow conditions: increasing and dividing the inlet flow rate. The finite volume method and Reynolds stress turbulence model were used to solve the averaged Navier–Stokes equations, whereas the Eulerian–Lagrangian approach and discrete phase model (DPM) were applied to track particles with a uniform diameter of 0.5–1.8 microns as the discrete phase. Owing to the low Stokes number and small and low-volume-fraction particles, a one-way coupling method was employed between air and the particles. The addition of an additional inlet reduced the static pressure in the center and downstream areas and increased the reverse flow velocity at the end of the cyclone. The installation of an additional inlet at 0.95D had the most positive effect on the separation efficiency, with an increase of 28.8% in the increasing flow rate case and 19.6% in the dividing flow rate case compared with the cyclone without an additional inlet. Furthermore, the increase in the separation efficiency of the submicron particles was greater than that of the larger particles in both flow distribution cases.
Personal respirable dust and respirable crystalline silica exposure in two shafts and a concentrator of a Zambian copper mine
Authors: Sifanu Mwaba , Kalebaila Kennedy K , Hayumbu Patrick , Nabiwa Lubinda , Linde Stephanus J L 
Published: January 2024

Abstract

Objectives
Since the 1920s, Zambia’s mining sector has experienced growth, which has increased the number of mine workers employed in the industry. Consequently, the potential for occupational exposure and prevalence of occupational diseases have also increased. Unfortunately, Zambia does not currently have legislative guidelines for workplace air monitoring and compliance. This study’s objectives were to evaluate copper miners’ personal exposure to respirable dust and respirable crystalline silica (RCS) and to assess workplace compliance using the European Standard for workplace air monitoring and measurement (EN689:2018).

Methods
This cross-sectional study collected 100 personal respirable dust exposure samples at a Zambian copper mine in 2023. These samples were weighed using NIOSH method 0600 and analyzed for crystalline silica using Fourier transform infrared spectroscopy (KBr pellet) (NIOSH method 7602). Additionally, 253 respirable dust exposure measurements collected at the mine between 2017 and 2022 were included for comparison.

Results
The median respirable dust exposure for the 2023 exposure measurements was 0.200 mg/m3 (95th percentile 2.871 mg/m3) compared to 0.400 mg/m3 (95th percentile 3.050 mg/m3) for the historic data. The median RCS exposure was 0.012 mg/m3 (95th percentile 0.163 mg/m3). Using EN689:2018, it was found that from 15 work areas, only six work areas complied with the standard for respirable dust exposure and only seven work areas complied with the standard for RCS exposure.

Conclusions
At the mining site, several work areas had substantial exposure to respirable dust and RCS. Therefore, management needs to prioritize these areas when implementing control measures to reduce dust exposure. For the Zambia mining industry to manage exposure to respirable dust and RCS, it is necessary to implement standardized monitoring strategies. This study has demonstrated that EN689:2018 can be used successfully to determine compliance among Zambian mining work areas.
The Effects of Mutation and Recombination Rate Heterogeneity on the Inference of Demography and the Distribution of Fitness Effects
Authors: Soni Vivak , Pfeifer Susanne P , Jensen Jeffrey D 
Published: January 2024

Abstract
Disentangling the effects of demography and selection has remained a focal point of population genetic analysis. Knowledge about mutation and recombination is essential in this endeavor; however, despite clear evidence that both mutation and recombination rates vary across genomes, it is common practice to model both rates as fixed. In this study, we quantify how this unaccounted for rate heterogeneity may impact inference using common approaches for inferring selection (DFE-alpha, Grapes, and polyDFE) and/or demography (fastsimcoal2 and δaδi). We demonstrate that, if not properly modeled, this heterogeneity can increase uncertainty in the estimation of demographic and selective parameters and in some scenarios may result in mis-leading inference. These results highlight the importance of quantifying the fundamental evolutionary parameters of mutation and recombination before utilizing population genomic data to quantify the effects of genetic drift (i.e. as modulated by demographic history) and selection; or, at the least, that the effects of uncertainty in these parameters can and should be directly modeled in downstream inference.
Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage
Authors: Zhang Bin , Wu Yuankang , Li Shoufan , Ren Wenjing , Yang Limei , Zhuang Mu , Lv Honghao , Wang Yong , Ji Jialei , Hou Xilin , Zhang Yangyong 
Published: January 2024

Abstract
Leaf color is an important agronomic trait in cabbage (Brassica oleracea L. var. capitata), but the detailed mechanism underlying leaf color formation remains unclear. In this study, we characterized a Brassica oleracea yellow-green leaf 2 (BoYgl-2) mutant 4036Y, which has significantly reduced chlorophyll content and abnormal chloroplasts during early leaf development. Genetic analysis revealed that the yellow-green leaf trait is controlled by a single recessive gene. Map-based cloning revealed that BoYgl-2 encodes a novel nuclear-targeted P-type PPR protein, which is absent in the 4036Y mutant. Functional complementation showed that BoYgl-2 from the normal-green leaf 4036G can rescue the yellow-green leaf phenotype of 4036Y. The C-to-U editing efficiency and expression levels of atpF, rps14, petL and ndhD were significantly reduced in 4036Y than that in 4036G, and significantly increased in BoYgl-2 overexpression lines than that in 4036Y. The expression levels of many plastid- and nuclear-encoded genes associated with chloroplast development in BoYgl-2 mutant were also significantly altered. These results suggest that BoYgl-2 participates in chloroplast C-to-U editing and development, which provides rare insight into the molecular mechanism underlying leaf color formation in cabbage.
Leaf variegation caused by plastome structural variation: an example from Dianella tasmanica
Authors: Zhou Shuaixi , Ma Kainan , Mower Jeffrey P , Liu Ying , Zhou Renchao 
Published: January 2024

Abstract
Variegated plants often exhibit plastomic heteroplasmy due to single-nucleotide mutations or small insertions/deletions in their albino sectors. Here, however, we identified a plastome structural variation in albino sectors of the variegated plant Dianella tasmanica (Asphodelaceae), a perennial herbaceous plant widely cultivated as an ornamental in tropical Asia. This structural variation, caused by intermolecular recombination mediated by an 11-bp inverted repeat flanking a 92-bp segment in the large single-copy region (LSC), generates a giant plastome (228 878 bp) with the largest inverted repeat of 105 226 bp and the smallest LSC of 92 bp known in land plants. It also generates an ~7-kb deletion on the boundary of the LSC, which eliminates three protein coding genes (psbA, matK, and rps16) and one tRNA gene (trnK). Albino sectors exhibit dramatic changes in expression of many plastid genes, including negligible expression of psbA, matK, and rps16, reduced expression of photosynthesis-related genes, and increased expression of genes related to the translational apparatus. Microscopic and ultrastructure observations showed that albino tissues were present in both green and albino sectors of the variegated individuals, and chloroplasts were poorly developed in the mesophyll cells of the albino tissues of the variegated individuals. These poorly developed chloroplasts likely carry the large and rearranged plastome, which is likely responsible for the loss of photosynthesis and albinism in the leaf margins. Considering that short repeats are relatively common in plant plastomes and that photosynthesis is not necessary for albino sectors, structural variation of this kind may not be rare in the plastomes of variegated plants.
A workflow for standardizing the analysis of highly resolved vessel tracking data
Authors: Mendo T , Mujal-Colilles A , Stounberg J , Glemarec G , Egekvist J , Mugerza E , Rufino M , Swift R , James M 
Published: January 2024

Abstract
Knowledge on the spatial and temporal distribution of the activities carried out in the marine environment is key to manage available space optimally. However, frequently, little or no information is available on the distribution of the largest users of the marine space, namely fishers. Tracking devices are being increasingly used to obtain highly resolved geospatial data of fishing activities, at intervals from seconds to minutes. However, to date no standardized method is used to process and analyse these data, making it difficult to replicate analysis. We develop a workflow to identify individual vessel trips and infer fishing activities from highly resolved geospatial data, which can be applied for large-scale fisheries, but also considers nuances encountered when working with small-scale fisheries. Recognizing the highly variable nature of activities conducted by different fleets, this workflow allows the user to choose a path that best aligns with the particularities in the fishery being analysed. A new method to identify anchoring sites for small-scale fisheries is also presented. The paper provides detailed code used in each step of the workflow both in R and Python language to widen the application of the workflow in the scientific and stakeholder communities and to encourage its improvement and refinement in the future.
The USDA Forest Service’s Forest Inventory and Analysis Database and the National Register of Champion Trees — A Potentially Symbiotic Relationship
Authors: Roesch Francis A , Schroeder Todd A , Price Charles A 
Published: January 2024

Abstract
This article shows how probability sampling and citizen science efforts can complement each other, using the USDA Forest Service’s Forest Inventory and Analysis (FIA) program and the ongoing search by the National Register of Champion Trees (NRCT) for the largest specimen of each naturally occurring tree species in the United States as an example. We develop a ratio statistic (Zs) that uses the difference in size of the largest tree of a species from each database to order the tree species according to the assumed ease with which a larger specimen than the current national champion might be found. Our results show ninety-two candidate species that have been recorded by FIA for which there is no national champion and sixty-five species for which a new champion should be easy to find. In a supplemental table, we show ninety-four species listed as observable by FIA in the NRCT but not recorded in the FIA sample.
Study Implications: An interest in forests and forestry is always accompanied by an interest in trees, especially very big trees. Two very different ways of learning about trees are analyzed concurrently in a way that reveals their complementarity. The two efforts are the probability sample, conducted by the USDA Forest Service’s Forest Inventory and Analysis (FIA) Program, and the citizen science effort known as the National Register of Champion Trees (NRCT). We develop a statistic that will help tree sleuths find champion trees and provide FIA practitioners with a quality control measure and an indication of which species would benefit from an increase in sample intensity.
Super super obesity with a BMI of 98 kg/m2: a case report
Authors: Al Jazzar Ragad I , Alzahrani Nayef , Eltayeb Mustafa M , Satti Ahmed K , Omar Hussain , Arrowaili Arief 
Published: January 2024

Abstract
Standards of care in regards to super-super obese patients are yet to be evolving due to the sparsity of this weight category along with the high morbidity and mortality rates attributed to it. We report a successful case of laparoscopic sleeve gastrectomy for a 35 years old lady with a body mass index (BMI) of 98 kg/m2.
Mapping Germanness in early 20th century USA: topic modeling and GIS within a small corpus framework
Authors: Wang Sijie , Kurzynski Maciej 
Published: January 2024

AbstractThe increased emphasis on language and ethnicity among German immigrants in the USA at the beginning of the 20th century resulted from inter-ethnic competition as well as assimilation pressures on Germans as a minority in American society. Following the unification of Germany and the improvement of German international status, Germans in America claimed superiority of German culture; middle-class advocates attempted to build a more united German-American community, fighting for a stronger voice on issues such as prohibition and German language education. These processes eventually led to the establishment of the National German-American Alliance in Philadelphia in 1901. The present article employs topic modeling and GIS techniques to examine the little-known conference proceedings of the Alliance and discuss Prince Heinrich “Henry” of Prussia’s 1902 visit to the USA. On the humanities side, we foreground the dynamics of the German diaspora who sought their own ethnic uniqueness and constructed historical memory during this period. On the digital side, we discuss different statistical evaluations of topic models as well as their applicability within a small corpus research framework.
Unsigned play by Milan Kundera? An authorship attribution study
Authors: Jungmannová Lenka , Plecháč Petr 
Published: January 2024

Abstract
In addition to being a widely recognized novelist, Milan Kundera has also authored three pieces for theatre: The Owners of the Keys (Majitelé klíčů 1961), The Blunder (Ptákovina 1967), and Jacques and his Master (Jakub a jeho pán 1971). In recent years, however, the hypothesis has been raised that Kundera was the true author of a fourth play, Juro Jánošík, first performed in a 1974 production under the name of Karel Steigerwald, who was Kundera’s student at the time. In this study, we make use of supervised machine learning to settle the question of authorship attribution in the case of Juro Jánošík, with results strongly supporting the hypothesis of Kundera’s authorship.
Coarsening natural deduction proofs II: finding gaunt proofs
Authors: Brauer Ethan 
Published: January 2024

Abstract
This paper is the second part of a series exploring how, given a proof, we can inductively transform it into a proof that contains no irrelevancies and is as strong as possible. In the prequel paper, I defined a weaker and a stronger notion of what counts as a proof with no irrelevancies, calling them perfect proofs and gaunt proofs, respectively. There, I showed how proofs in core logic and classical core logic can be transformed into perfect proofs. In this paper I study gaunt proofs. I show how proofs in core logic can be inductively transformed into gaunt core proofs, but that this property fails for the natural deduction system of classical core logic.
Brooklyn plots to identify co-expression dysregulation in single cell sequencing
Authors: Patil Arun H , McCall Matthew N , Halushka Marc K 
Published: January 2024

Abstract
Altered open chromatin regions, impacting gene expression, is a feature of some human disorders. We discovered it is possible to detect global changes in genomically-related adjacent gene co-expression within single cell RNA sequencing (scRNA-seq) data. We built a software package to generate and test non-randomness using ‘Brooklyn plots’ to identify the percent of genes significantly co-expressed from the same chromosome in ∼10 MB intervals across the genome. These plots establish an expected low baseline of co-expression in scRNA-seq from most cell types, but, as seen in dilated cardiomyopathy cardiomyocytes, altered patterns of open chromatin appear. These may relate to larger regions of transcriptional bursting, observable in single cell, but not bulk datasets.
Combined “Test and Treat” Campaigns for Human Immunodeficiency Virus, Hepatitis B, and Hepatitis C: A Systematic Review to Provide Evidence to Support World Health Organization Treatment Guidelines
Authors: Beard Natasha , Hill Andrew 
Published: January 2024

Abstract

Background
Worldwide, more than 39 million individuals are living with human immunodeficiency virus (HIV), 296 million with chronic hepatitis B (HBV), and 58 million with chronic hepatitis C (HCV). Despite successful treatments for these blood-borne viruses (BBVs), &gt;1.7 million people die per annum. To combat this, the World Health Organization recommended implementing triple testing for HIV, HBV, and HCV. This systematic review aims to provide evidence for this policy, by identifying the prevalence of these BBVs and discussing the costs of available triple tests.

Methods
Medline, Embase, and Global Health were searched to identify articles published between 1 January and 24 February 2023. Included studies reported the prevalence of HIV (anti-HIV 1/2 antibodies), HBV (hepatitis B surface antigen) and HCV (anti-HCV antibodies). Results were stratified into risk groups: blood donors, general population, healthcare attendees, individuals experiencing homelessness, men who have sex with men, people who use drugs, pregnant people, prisoners, and refugees and immigrants.

Results
One hundred seventy-five studies sampling &gt;14 million individuals were included. The mean prevalence of HIV, HBV, and HCV was 0.22% (standard deviation [SD], 7.71%), 1.09% (SD, 5.80%) and 0.65% (SD, 14.64%) respectively. The mean number of individuals testing positive for at least 1 BBV was 1.90% (SD, 16.82%). Therefore, under triple testing, for every individual diagnosed with HIV, another 5 would be diagnosed with HBV and 3 with HCV. Testing for all 3 viruses is available for US$2.48, marginally more expensive than the lowest-priced isolated HIV test ($1.00).

Conclusions
This article highlights a potential avenue for healthcare improvement by implementing combination testing programs. Hopefully, this will help to achieve the Sustainable Development Goal of elimination of these BBV epidemics by 2030.
Price of Regulations: Regulatory Costs and the Cross-section of Stock Returns
Authors: Ince Baris , Ozsoylev Han 
Published: January 2024

Abstract
Regulations introduce significant fixed costs and add to operating leverage. Fixed regulatory costs that contribute to operating leverage should generate a risk premium. To explore whether such a premium exists, we introduce a measure of “regulatory operating leverage” that reflects the importance of fixed regulatory costs in a firm’s cost structure. Regulatory operating leverage predicts stock returns in the cross-section, and a zero-cost high-low regulatory operating leverage strategy generates positive and significant risk-adjusted return. Finally, the impact of regulatory operating leverage on returns is due to the (systematic) risk contribution of fixed regulatory costs.
Altered sleep architecture following consecutive nights of presleep alcohol
Authors: McCullar Katie S , Barker David H , McGeary John E , Saletin Jared M , Gredvig-Ardito Caroline , Swift Robert M , Carskadon Mary A 
Published: January 2024

Abstract

Study Objectives
Alcohol consumption before sleep decreases sleep latency, explaining the common use of alcohol as a sleep aid. The full impact of alcohol on sleep architecture is not well understood, particularly the potential cumulative effects of presleep alcohol consumption across consecutive nights. Here, we describe the effects of presleep alcohol on sleep architecture across three consecutive nights.

Methods
Thirty adult participants took part in a crossover, within-participants study consisting of two sets of three consecutive nights of in-lab polysomnography. For each series of nights, participants drank one of the two beverages: a mixer only or a mixer plus alcohol (targeting a BrAC of 0.08 mg/L), ending 1 hour before lights out. Polysomnography (PSG) was used to stage sleep, and standard sleep variables were extracted. Linear mixed-effect analysis and generalized additive modeling were used to examine the effect of alcohol on sleep architecture.

Results
Alcohol before sleep increased the rate of slow wave sleep (SWS) accumulation across all three nights and decreased the rate of rapid eye movement (REM) sleep accumulation at the start of each night. Alcohol also decreased the total amount of REM sleep but did not affect the total amount of SWS each night.

Conclusions
These data indicate that drinking alcohol before sleep substantially affects sleep architecture, including changes to the rate of accumulation of SWS and REM sleep. We show that alcohol disrupts normal sleep architecture, leading to a significant decrease in REM sleep; thus, the use of alcohol as a sleep aid remains a public health concern.
GRK5 promoted renal fibrosis via HDAC5/Smad3 signaling pathway
Authors: Xiang Huiling , Huang Jing , Song Anni , Liu Feng , Xiong Jing , Zhang Chun 
Published: January 2024

AbstractRenal fibrosis is a common pathological feature of chronic kidney diseases (CKD), poses a significant burden in the aging population, and is a major cause of end‐stage renal disease (ESRD). In this study, we investigated the role of G protein‐coupled receptor kinases (GRKs) 5 in the pathogenesis of renal fibrosis. GRK5 is a serine/threonine kinase that regulates G protein‐coupled receptor (GPCR) signaling. GRK5 has been shown to play a role in various diseases including cardiac disorders and cancer. However, the role of GRK5 in renal fibrosis remains largely unknown. Our finding revealed that GRK5 was significantly overexpressed in renal fibrosis. Specifically, GRK5 was transferred into the nucleus via its nuclear localization sequence to regulate histone deacetylases (HDAC) 5 expression under renal fibrosis. GRK5 acted as an upstream regulator of HDAC5/Smad3 signaling pathway. HDAC5 regulated and prevented the transcriptional activity of myocyte enhancer factor 2A (MEF2A) to repress the transcription of Smad7 which leading to the activation of Smad3. These findings first revealed that GRK5 may be a potential therapeutic target for the treatment of renal fibrosis. Inhibition of GRK5 activity may be a promising strategy to attenuate the progression of renal fibrosis.
Imaging of chronic rhinosinusitis with nasal polyps in the era of biological therapies
Authors: Poto Remo , Pelaia Corrado , di Salvatore Antonio , Saleh Hesham , Scadding Guy W. , Varricchi Gilda 
Published: January 2024

Purpose of review
Chronic rhinosinusitis (CRS) is a chronic inflammatory disorder of the sinonasal cavities classified into two major phenotypes: CRS with nasal polyps (CRSwNP) and without nasal polyps (CRSsNP). The diagnosis of CRS is based on clinical symptoms associated with imaging and/or nasal endoscopy findings of mucosal inflammation.

Recent findings
Recently, novel biological therapies have emerged as therapeutic options for CRSwNP. Imaging is helpful in deciding whether surgery is likely to be beneficial and in guiding surgery. It can also help demonstrate a clinical response to medical therapy. However, specific guidelines concerning the role of imaging in CRwNP are lacking.

Summary
This article provides a comprehensive and critical multidisciplinary review of the role of conventional radiology, computed tomography (CT), and magnetic resonance imaging (MRI) in the diagnosis and characterization of CRSwNP. Since the complete characterization of nasal polyps on CT or MR images is very challenging, we provide a critical review of the best imaging methods and essential reporting elements used to assess nasal polyps.