bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes
Authors: Ahedor Believe , Otgonsuren Davaajav , Zhyldyz Atambekova , Guswanto Azirwan , Ngigi Noel Muthoni Mumbi , Valinotti Maria Fátima Rodríguez , Kothalawala Hemal , Kalaichelvan Nizanantha , Silva Seekkuge Susil Priyantha , Kothalawala Hemali , Acosta Tomás Javier , Sivakumar Thillaiampalam , Yokoyama Naoaki 
Published: November 2023

Abstract
Background
Theileria equi causes equine piroplasmosis, an economically significant disease that affects horses and other equids worldwide. Based on 18S ribosomal RNA (18S rRNA sequences), T. equi can be classified into five genotypes: A, B, C, D, and E. These genotypes have implications for disease management and control. However, no conventional polymerase chain reaction (PCR) assays are available to differentiate the genotypes of T. equi. To overcome this limitation, we developed and evaluated PCR assays specific for the detection of each T. equi genotype.

Methods
A pair of forward and reverse primers, specifically targeting the 18S rRNA sequence of each genotype, was designed. The genotype-specific PCR assays were evaluated for their specificity using plasmids containing inserts of the 18S rRNA sequence of each genotype. Subsequently, the assays were tested on 270 T. equi-positive equine blood DNA samples (92 from donkeys in Sri Lanka and 178 from horses in Paraguay). 18S rRNA sequences derived from the PCR amplicons were analyzed phylogenetically.

Results
Each genotype-specific PCR assay accurately targeted the intended genotype, and did not produce any amplicons when 18S rRNA from other T. equi genotypes or genomic DNA of Babesia caballi or uninfected horse blood was used as the template. Previous studies employing PCR sequencing methods identified T. equi genotypes C and D in the Sri Lankan samples, and genotypes A and C in the Paraguayan samples. In contrast, our PCR assay demonstrated exceptional sensitivity by detecting four genotypes (A, C, D, and E) in the Sri Lankan samples and all five genotypes in the Paraguayan samples. All the Sri Lankan samples and 93.3% of the Paraguayan samples tested positive for at least one genotype, further emphasizing the sensitivity of our assays. The PCR assays also had the ability to detect co-infections, where multiple genotypes in various combinations were detected in 90.2% and 22.5% of the Sri Lankan and Paraguayan samples, respectively. Furthermore, the sequences obtained from PCR amplicons clustered in the respective phylogenetic clades for each genotype, validating the specificity of our genotype-specific PCR assays.

Conclusions
The genotype-specific PCR assays developed in the present study are reliable tools for the differential detection of T. equi genotypes.

Graphical abstract
Cost-effectiveness of continuous glucose monitoring with FreeStyle Libre® in Brazilian insulin-treated patients with types 1 and 2 diabetes mellitus
Authors: Bahia Luciana , Mello Karla F. , Lemos Lívia Lovato Pires , Costa Naiane Lima , Mulinari Eduardo , Malerbi Domingos A. 
Published: November 2023

Abstract
Background
Hypoglycemia is a barrier to optimal glucose control in the treatment of both type 1 (T1DM) and type 2 diabetes mellitus (T2DM). Blood glucose monitoring is essential in diabetes management. Inappropriate glucose management is associated with high mortality and morbidity. FreeStyle Libre® (FSL) is a continuous glucose monitoring (CGM) system that provides effective, safe, and convenient glucose monitoring, without routine finger pricking. This study aims to estimate the incremental cost-effectiveness ratio (ICER) of the FSL system in comparison to conventional Self-monitoring of blood glucose (SMBG) in T1DM and T2DM patients that require intensive insulin therapy.

Methods
A decision-tree model was developed to compare the cost-effectiveness ratio between FSL and conventional SMBG from the perspective of the Brazilian Public Healthcare System (SUS). The model captures the cumulative rates of acute complications such as severe hypoglicemia and diabetic ketoacidosis, per-event costs, and quality-adjusted life-years (QALYs) gained over a 1-year time horizon in adult and pediatric patients (≥ 4 years old) with T1DM or T2DM. Inputs from the Brazilian health databases, clinical trials, and real-world data were used in the study.

Results
The results demonstrated that, regarding solely severe hypoglicemia and diabetic ketoacidosis events, T1DM have a QALY difference of 0.276, a cost difference of R$ 7.255, and an ICER of R$ 26,267.69 per QALY gained for CGM with FSL, when compared to conventional SMBG. T2DM results demonstrated equally a QALY difference of 0.184, a cost difference of R$ 7290, and an ICER of R$ 39,692.67 per QALY gained, in favour of CGM with FSL.

Conclusion
Our findings demonstrated that FSL is cost-effective in T1DM and T2DM for acute diabetic complications, from a SUS perspective. CGM with FSL can promote safe, convenient, and cost-effective glucose monitoring, therefore contributing to the improvement of the incidence of complications and quality of life.
Dataset of the impact of food insecurity on health outcomes in sub-Saharan Africa
Authors: Beyene Sisay Demissew 
Published: November 2023

Abstract
Objectives
The 2030 Sustainable Development Goals (SDGs) have goals and targets, including food insecurity and health outcomes. Hence, information about socioeconomic variables that determine the health outcomes of people is essential for health-related research, planning, and policy development. Therefore, this data paper aims to present (describe) the dataset of eight socioeconomic variables for 31 sub-Saharan Africa (SSA) countries from 2001 to 2018.

Data descriptions
The dataset was official information obtained via open online sources from the Food and Agricultural Organization (FAO), the World Bank (WB), and the United Nations Development Programme (UNDP). It included 558 observations and eight variables, such as life expectancy (LEXP), infant mortality (INFMOR), the prevalence of undernourishment (PRUND), average dietary energy supply (AVRDES), Gross domestic product per capita (GDPPC), general government health expenditure (GOVEXP), urbanisation (URBAN), and mean years of schooling (MNSCHOOL). Moreover, all the data estimation is conducted by Statistical Software (STATA) version 15. This paper achieved its intended objective with a detailed and understandable description.
Imiquimod-induced pruritus in female wild-type and knockin Wistar rats: underscoring behavioral scratching in a rat model for antipruritic treatments
Authors: Lariosa-Willingham Karen , Leonoudakis Dmitri , Simon Florian , Walker Kendall , Guillaume Philippe , Warren Liling , Stratton Jennifer 
Published: November 2023

Abstract
Objectives
Animal models of skin disease are used to evaluate therapeutics to alleviate disease. One common clinical dermatological complaint is pruritus (itch), but there is a lack of standardization in the characterization of pre-clinical models and scratching behavior, a key itch endpoint, is often neglected. One such model is the widely used imiquimod (IMQ) mouse model of psoriasis. However, it lacks characterized behavioral attributes like scratching, nor has widely expanded to other species like rats.
Given these important attributes, this study was designed to broaden the characterization beyond the expected IMQ-induced psoriasis-like skin inflammatory skin changes and to validate the role of a potential therapeutic agent for pruritus in our genetic rat model. The study included female Wistar rats and genetically modified knockin (humanized proteinase-activated receptor 2 (F2RL1) female rats, with the widely used C57BL/6 J mice as a methodology control for typical IMQ dosing.

Results
We demonstrate that the IMQ model can be reproduced in rats, including their genetically modified derivatives, and how scratching can be used as a key behavioral endpoint. We systemically delivered an anti-PAR2 antibody (P24E1102) which reversed scratching bouts—validating this behavioral methodology and have shown its feasibility and value in identifying effective antipruritic drugs.
A 2-decade bibliometric analysis of epigenetics of cardiovascular disease: from past to present
Authors: Mao Yukang , Zhao Kun , Chen Nannan , Fu Qiangqiang , Zhou Yimeng , Kong Chuiyu , Li Peng , Yang Chuanxi 
Published: November 2023

Abstract
Background
Cardiovascular disease (CVD) remains a major health killer worldwide, and the role of epigenetic regulation in CVD has been widely studied in recent decades. Herein, we perform a bibliometric study to decipher how research topics in this field have evolved during the past 2 decades.

Results
Publications on epigenetics in CVD produced during the period 2000–2022 were retrieved from the Web of Science Core Collection (WoSCC). We utilized Bibliometrix to build a science map of the publications and applied VOSviewer and CiteSpace to assess co-authorship, co-citation, co-occurrence, and bibliographic coupling. In total, 27,762 publications were included for bibliometric analysis. The yearly amount of publications experienced exponential growth. The top 3 most influential countries were China, the United States, and Germany, while the most cited institutions were Nanjing Medical University, Harbin Medical University, and Shanghai Jiao Tong University. Four major research trends were identified: (a) epigenetic mechanisms of CVD; (b) epigenetics-based therapies for CVD; (c) epigenetic profiles of specific CVDs; and (d) epigenetic biomarkers for CVD diagnosis/prediction. The latest and most important research topics, including “nlrp3 inflammasome”, “myocardial injury”, and “reperfusion injury”, were determined by detecting citation bursts of co-occurring keywords. The most cited reference was a review of the current knowledge about how miRNAs recognize target genes and modulate their expression and function.

Conclusions
The number and impact of global publications on epigenetics in CVD have expanded rapidly over time. Our findings may provide insights into the epigenetic basis of CVD pathogenesis, diagnosis, and treatment.
Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair
Authors: Li Guoling , Yang Xiaohui , Luo Xinxin , Wu Zhenfang , Yang Huaqiang 
Published: November 2023

Abstract
Background
Gene knock‐in (KI) in animal cells via homology‐directed repair (HDR) is an inefficient process, requiring a laborious work for screening from few modified cells. HDR tends to occur in the S and G2/M phases of cell cycle; therefore, strategies that enhance the proportion of cells in these specific phases could improve HDR efficiency.

Results
We used various types of cell cycle inhibitors to synchronize the cell cycle in S and G2/M phases in order to investigate their effect on regulating CRISPR/Cas9-mediated HDR. Our results indicated that the four small molecules—docetaxel, irinotecan, nocodazole and mitomycin C—promoted CRISPR/Cas9-mediated KI with different homologous donor types in various animal cells. Moreover, the small molecule inhibitors enhanced KI in animal embryos. Molecular analysis identified common signal pathways activated during crosstalk between cell cycle and DNA repair. Synchronization of the cell cycle in the S and G2/M phases results in CDK1/CCNB1 protein accumulation, which can initiate the HDR process by activating HDR factors to facilitate effective end resection of CRISPR-cleaved double-strand breaks. We have demonstrated that augmenting protein levels of factors associated with the cell cycle via overexpression can facilitate KI in animal cells, consistent with the effect of small molecules.

Conclusion
Small molecules that induce cell cycle synchronization in S and G2/M phases promote CRISPR/Cas9-mediated HDR efficiency in animal cells and embryos. Our research reveals the common molecular mechanisms that bridge cell cycle progression and HDR activity, which will inform further work to use HDR as an effective tool for preparing genetically modified animals or for gene therapy.
Design and analysis of wireless data center network topology HCDCN based on VLC
Authors: Zhang Qingfang , Du Xiaoyu , Li Jie , Han Zhijie 
Published: November 2023

AbstractWith the rapid development of the Internet of Things, big data, cloud computing and other industries, the data center network, as the core infrastructure of the computing industry, is becoming increasingly important. Wireless communication technology is developing rapidly. This paper uses visible light wireless communication technology to build a wireless data center network, solving the problems of complex wiring and expansion difficulties in the existing data center network.Aiming at the problems of insufficient spectrum, low bandwidth utilization and large energy consumption in the existing wireless data center, this paper adopts the visible light wireless communication technology based on LED, and proposes a new wireless data center network structure HCDCN. Firstly, work have been done to analyze the feasibility of introducing data centers in this technology, and transform server racks in combination with the characteristics of the technology. Secondly, the construction process of the structure is introduced and a new coding rule is proposed. In order to increase the routing efficiency and fault tolerance, the rack top link routing path is added, and an efficient routing algorithm is designed. Finally, the aggregate throughput of HCDCN under different scales is obtained through experiments, and the network performance of HCDCN structure is compared with that of OWCells and Mesh structure. Experiments have proved that HCDCN's network performance is better than the other two structures under the same conditions. The new wireless data center network proposed in this paper has largely alleviated the bandwidth shortage of hotspot servers.
A case–control comparison of acute-phase peripheral blood gene expression in participants diagnosed with minor ischaemic stroke or stroke mimics
Authors: Moxon Joseph V. , Calcino Andrew , Kraeuter Ann-Katrin , Phie James , Anderson Georgina , Standley Glenys , Sealey Cindy , Jones Rhondda E. , Field Matt A. , Golledge Jonathan 
Published: November 2023

Abstract
Background
Past studies suggest that there are changes in peripheral blood cell gene expression in response to ischaemic stroke; however, the specific changes which occur during the acute phase are poorly characterised. The current study aimed to identify peripheral blood cell genes specifically associated with the early response to ischaemic stroke using whole blood samples collected from participants diagnosed with ischaemic stroke (n = 29) or stroke mimics (n = 27) following emergency presentation to hospital. Long non-coding RNA (lncRNA), mRNA and micro-RNA (miRNA) abundance was measured by RNA-seq, and the consensusDE package was used to identify genes which were differentially expressed between groups. A sensitivity analysis excluding two participants with metastatic disease was also conducted.

Results
The mean time from symptom onset to blood collection was 2.6 h. Most strokes were mild (median NIH stroke scale score 2.0). Ten mRNAs (all down-regulated in samples provided by patients experiencing ischaemic stroke) and 30 miRNAs (14 over-expressed and 16 under-expressed in participants with ischaemic stroke) were significantly different between groups in the whole cohort and sensitivity analyses. No significant over-representation of gene ontology categories by the differentially expressed genes was observed. Random forest analysis suggested a panel of differentially expressed genes (ADGRG7 and miRNAs 96, 532, 6766, 6798 and 6804) as potential ischaemic stroke biomarkers, although modelling analyses demonstrated that these genes had poor diagnostic performance.

Conclusions
This study provides evidence suggesting that the early response to minor ischaemic stroke is predominantly reflected by changes in the expression of miRNAs in peripheral blood cells. Further work in independent cohorts particularly in patients with more severe stroke is needed to validate these findings and investigate their clinical relevance.
Effect of coaches’ interpersonal style on young athletes’ individual resilience and team adherence intention: a season-long investigation
Authors: Llanos-Muñoz Rubén , Pulido Juan J. , Nobari Hadi , Raya-González Javier , López-Gajardo Miguel A. 
Published: November 2023

Abstract
Background
In the sports context, coaches must be able to improve their players physically, psychologically, and socially. Hence, a fundamental part of this process is the athlete’s individual resilience (IR).

Methods
Three hundred and fifteen youth team-sport players (boys: n = 283; Mage = 16.02, SD = 0.56; and girls: n = 32; Mage = 15.92, SD = 0.62) completed the measures of coach’s interpersonal style, individual resilience, perceived performance, and team adherence intention (intention to remain on the same team the following year) twice (Time 1: mid-season; Time 2: end-season). Structural equation modeling was used to test the relationships between variables.

Results
The results showed that coach support was positively related to IR (p < 0.001) and, in turn, IR to individual (p < 0.01) and team performance (p < 0.05) at Time 1, and to individual performance (p < 0.001) and team adherence intention at Time 2 (p < 0.01). In addition, team performance at Time 2 was positively related to team adherence intention (p < 0.001). Finally, a mediating effect of IR was observed between interpersonal coaching style, individual and team performance, and team adherence intention.

Conclusions
These results show the importance of a supportive interpersonal coaching style to foster athletes’ levels of resilience, which could have positive consequences in performance (individual and team) and team adherence intention.
Platelet-to-Lymphocyte Ratio Might Predict the Response to Dupilumab Treatment for Patients with Nasal Polyposis
Authors: Brkic Faris F. , Liu David T. , Rücklinger Iris , Campion Nicholas James , Bartosik Tina Josefin , Vyskocil Erich , Stanek Victoria , Tu Aldine , Gangl Katharina , Schneider Sven 
Published: November 2023

Background Dupilumab is a monoclonal antibody against interleukin 4 receptor alpha and has proven to be clinically effective in treating patients with chronic rhinosinusitis with nasal polyps (CRSwNP). However, a certain number of patients are non- or partial responders. This study aims to investigate the relevance of inflammatory markers with regard to therapy response to dupilumab in CRSwNP patients. Methods All patients with CRSwNP treated with dupilumab at a tertiary healthcare center with available pretreatment inflammatory markers were included. The values of pretreatment neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) were associated with the outcome. Patients were stratified according to the respective median value (> median was considered high). The binary logistic regression was performed with regard to total treatment response (post-treatment total nasal polyp score (NPS) 0). Results A total of 65 CRSwNP patients with available pretreatment peripheral blood values were included in the study. The mean pre- and post-treatment total NPS values were 4.3 ± 1.9 and 1.2 ± 1.6, respectively. High PLR (> 131.2) was independently associated with a 3.9-fold higher probability of reaching the NPS value of 0 in the multivariable analysis. On the other hand, High NLR (> 1.9) did not significantly associate with the outcome. Conclusions The current study provides insights into the potential positive predictive value of the high PLR (> 131.2) in CRSwNP patients regarding treatment with dupilumab. There is a need for further prospective studies for validation of these results, especially in cohorts of patients with severe CRSwNP.
The morphology of the femoral posterior condyle affects the external rotation of the femur
Authors: Hijikata Hiroki , Tanifuji Osamu , Mochizuki Tomoharu , Sato Takashi , Watanabe Satoshi , Katsumi Ryota , Hokari Sho , Kawashima Hiroyuki 
Published: November 2023

AbstractPurposeThe purpose of this study was to identify factors related to the external rotation of the femur during knee flexion.MethodsThree‐dimensional (3D) digital models of the femur and tibia were reconstructed from computed tomography images of 41 healthy Japanese subjects. Thirteen parameters related to femoral and tibial morphology and alignment of the lower extremities were evaluated, including the inclination angle of the posterior lateral and medial femoral condyles, the ratio of the medial and lateral posterior condyle radii approximated as spheres, the spherical condylar angle, the posterior condylar angle, the medial and lateral posterior tibial slope, the difference of medial and lateral posterior tibial slope, the tibiofemoral rotation angle, the 3D femorotibial angle, the 3D hip‐knee‐ankle angle, and the passing point of the weight‐bearing line (medial–lateral and anterior–posterior). The rotation angle of the femur relative to the tibia during squatting was investigated using a 3D to 2D image matching technique and the relationships with the13 parameters were determined.ResultsThe femur externally rotated substantially up to 20° of knee flexion (9.2° ± 3.7°) and gently rotated after 20° of knee flexion (12.8° ± 6.2°). The external rotation angle at 20°–120° of knee flexion correlated with the spherical condylar angle, the tibiofemoral rotation angle and the inclination angle of the posterior medial condyles (correlation coefficient; 0.506, 0.364, 0.337, respectively).ConclusionThe parameter that was most related to the external rotation of the femur during knee flexion was the spherical condylar angle.Level of EvidenceIV
Ultra-low tidal volume ventilation during cardiopulmonary resuscitation shows no mitigating effect on pulmonary end-organ damage compared to standard ventilation: insights from a porcine model
Authors: Mohnke Katja , Conzelmann Philipp , Renz Miriam , Riedel Julian , Rissel René , Urmann Andrea , Hain Johanna , Duenges Bastian , Ziebart Alexander , Ruemmler Robert 
Published: November 2023

Abstract
Objective
This study aimed to determine whether ultra-low tidal volume ventilation (ULTVV) applied during cardiopulmonary resuscitation (CPR) compared with standard ventilation (intermittent positive pressure ventilation, IPPV) can reduce pulmonary end-organ damage in the post-resuscitation period.

Methods
A prospective, randomized trial was conducted using a porcine model (n = 45). The animals were divided into three groups: IPPV, ULTVV, and a sham control group. Juvenile male pigs underwent CPR after inducing ventricular fibrillation and received the designated ventilation intervention [IPPV: tidal volume 6–8 ml per kilogram body weight (ml/kg BW), respiratory rate 10/min, FiO2 1.0; ULTVV: tidal volume 2–3 ml/kg BW, respiratory rate 50/min, FiO2 1.0]. A 20-h observation period followed if return of spontaneous circulation was achieved. Histopathological examination using the diffuse alveolar damage scoring system was performed on postmortem lung tissue samples. Arterial and venous blood gas analyses and ventilation/perfusion measurements via multiple inert gas elimination technique (MIGET) were repeatedly recorded during the experiment.

Results
Out of the 45 experiments conducted, 28 animals were excluded based on predefined criteria. Histopathological analysis showed no significant differences in lung damage between the ULTVV and IPPV groups. ULTVV demonstrated adequate oxygenation and decarboxylation. MIGET measurements during and after resuscitation revealed no significant differences between the intervention groups.

Conclusion
In the short-term follow-up phase, ULTVV demonstrated similar histopathological changes and functional pulmonary parameters compared to standard ventilation. Further research is needed to investigate the long-term effects and clinical implications of ULTVV in resuscitation settings.