bluuebunny/crossref_metadata_2025_split huggingface dataset:
A bibliometric analysis of m6A methylation in viral infection from 2000 to 2022
Authors: Tao Xing , Wang Gang , Wei Wudi , Su Jinming , Chen Xiu , Shi Minjuan , Liao Yinlu , Qin Tongxue , Wu Yuting , Lu Beibei , Liang Hao , Ye Li , Jiang Junjun 
Published: January 2024

Abstract
Background
N6-methyladenosine (m6A) methylation has become an active research area in viral infection, while little bibliometric analysis has been performed. In this study, we aim to visualize hotspots and trends using bibliometric analysis to provide a comprehensive and objective overview of the current research dynamics in this field.

Methods
The data related to m6A methylation in viral infection were obtained through the Web of Science Core Collection form 2000 to 2022. To reduce bias, the literature search was conducted on December 1, 2022. Bibliometric and visual analyzes were performed using CiteSpace and Bibliometrix package. After screening, 319 qualified records were retrieved.

Results
These publications mainly came from 28 countries led by China and the United States (the US), with the US ranking highest in terms of total link strength.The most common keywords were m6A, COVID-19, epitranscriptomics, METTL3, hepatitis B virus, innate immunity and human immunodeficiency virus 1. The thematic map showed that METTL3, plant viruses, cancer progression and type I interferon (IFN-I) reflected a good development trend and might become a research hotspot in the future, while post-transcriptional modification, as an emerging or declining theme, might not develop well.

Conclusions
In conclusion, m6A methylation in viral infection is an increasingly important topic in articles. METTL3, plant viruses, cancer progression and IFN-I may still be research hotspots and trends in the future.
Effects of physical activity levels on characteristic pain in temporomandibular dysfunctions: a cross-sectional study
Authors: Chun Youngwoo , Jo Jung Hwan , Park Ji Woon 
Published: January 2024

Abstract
Background
Physical activity is known to influence the symptoms of a variety of pain disorders including fibromyalgia and osteoarthritis although the underlying mechanism is not fully understood. In spite of the high prevalence of temporomandibular disorders (TMD), no previous study has objectively evaluated the relationship between TMD and general physical activity. This study aims to investigate the influence of physical activity on pain and disability from TMD, considering various confounders including sleep, systemic inflammation, psychosocial disturbances, and widespread pain.

Methods
This observational cross-sectional study is based on consecutive samples of 100 TMD patients (22 with high pain disability and 78 with low pain disability level). Physical activity levels were assessed with actigraph. Level of pain and disability were evaluated using the Graded Chronic Pain Scale. Hematologic examinations including inflammatory biomarkers were assessed and comorbidities were investigated with validated questionnaires. Differences were analyzed according to disability level.

Results
Patients with high disability level spent significantly more time doing both moderate (p = 0.033) and vigorous (p = 0.039) level physical activity. Light physical activity, on the other hand, was associated with low disability but the difference did not reach statistical significance. Time spent in light physical activity was significantly associated with high levels of pain and disability (p = 0.026, β = −0.001) and time spent in vigorous physical activity had significant predictive power (cutoff value 2.5 min per week, AUC 0.643, p = 0.041). Scores of the Jaw Function Limitation Score-20 (p = 0.001), present McGill Pain Score (p = 0.010), and number of people potentially diagnosed with fibromyalgia (p = 0.033) were significantly higher in the high disability group.

Conclusions
Moderate or vigorous physical activity is associated with worse TMD symptoms while light physical activity may be beneficial. Further research related to the amount and frequency of physical activity is necessary to establish clinical guidelines for TMD.

Trial registration
clinical trial registration of the Clinical Research Information Service of Republic of Korea (number KCT0007107).
Pathways to opioid use and implications for prevention: voices of young adults in recovery
Authors: Ballard Parissa J. , Arnold Taylor J. , Vidrascu Elena M. , Hernandez Guadalupe C. , Ozer Emily , Wolfson Mark , Lassiter Rebekah , Nayyar Himani , Daniel Stephanie S. 
Published: January 2024

Abstract
Background
Opioid use remains a major public health issue, especially among young adults. Despite investment in harm reduction and supply-side strategies such as reducing overprescribing and safe medication disposal, little is known about demand-side issues, such as reasons for use and pathways to opioid use. Adolescents and young adults who struggle with opioid use disorder (OUD) are multifaceted individuals with varied individual histories, experiences, challenges, skills, relationships, and lives.

Methods
To inform the development of prevention strategies that hold promise for addressing opioid use, this study employs brief structured surveys and semi-structured in-depth interviews with 30 young adults (ages 18–29; 19 female, 23 White, 16 from Suburban areas) in recovery from OUD. For survey data, we used descriptive statistics to summarize the means and variance of retrospectively reported risk and protective factors associated with opioid use. For in-depth interview data, we used a combination of thematic analysis and codebook approaches to generate common themes and experiences shared by participants.

Results
Surveys revealed that the most endorsed risk factors pertained to emotions (emotional neglect and emotional abuse) followed by sexual abuse, physical abuse, and physical neglect. Themes generated from qualitative analyses reveal challenging experiences during adolescence, such as unaddressed mental health, social, and emotional needs, which were often reported as reasons for opioid initiation and use. Through surveys and interviews, we also identified positive assets, such as skills and social relationships that were present for many participants during adolescence.

Conclusion
Implications include the need for universal prevention strategies that include emotion-focused interventions and supports alongside current harm reduction and environmental strategies to regulate prescriptions; the potential utility of more emotion-focused items being included on screening tools; and more voices of young people in recovery.
Early management of adult traumatic spinal cord injury in patients with polytrauma: a consensus and clinical recommendations jointly developed by the World Society of Emergency Surgery (WSES) & the European Association of Neurosurgical Societies (EANS)
Authors: Picetti Edoardo , Demetriades Andreas K. , Catena Fausto , Aarabi Bizhan , Abu-Zidan Fikri M. , Alves Oscar L. , Ansaloni Luca , Armonda Rocco A. , Badenes Rafael , Bala Miklosh , Balogh Zsolt J. , Barbanera Andrea , Bertuccio Alessandro , Biffl Walter L. , Bouzat Pierre , Buki Andras , Castano-Leon Ana Maria , Cerasti Davide , Citerio Giuseppe , Coccolini Federico , Coimbra Raul , Coniglio Carlo , Costa Francesco , De Iure Federico , Depreitere Bart , Fainardi Enrico , Fehlings Michael J. , Gabrovsky Nikolay , Godoy Daniel Agustin , Gruen Peter , Gupta Deepak , Hawryluk Gregory W. J. , Helbok Raimund , Hossain Iftakher , Hutchinson Peter J. , Iaccarino Corrado , Inaba Kenji , Ivanov Marcel , Kaprovoy Stanislav , Kirkpatrick Andrew W. , Klein Sam , Kolias Angelos , Konovalov Nikolay A. , Lagares Alfonso , Lippa Laura , Loza-Gomez Angelica , Luoto Teemu M. , Maas Andrew I. R. , Maciejczak Andrzej , Maier Ronald V. , Marklund Niklas , Martin Matthew J. , Melloni Ilaria , Mendoza-Lattes Sergio , Meyfroidt Geert , Munari Marina , Napolitano Lena M. , Okonkwo David O. , Otomo Yasuhiro , Papadopoulos Marios C. , Petr Ondra , Peul Wilco C. , Pudkrong Aichholz K. , Qasim Zaffer , Rasulo Frank , Reizinho Carla , Ringel Florian , Rizoli Sandro , Rostami Elham , Rubiano Andres M. , Russo Emanuele , Sarwal Aarti , Schwab Jan M. , Servadei Franco , Sharma Deepak , Sharif Salman , Shiban Ehab , Shutter Lori , Stahel Philip F. , Taccone Fabio S. , Terpolilli Nicole A. , Thomé Claudius , Toth Peter , Tsitsopoulos Parmenion P. , Udy Andrew , Vaccaro Alexander R. , Varon Albert J. , Vavilala Monica S. , Younsi Alexander , Zackova Monika , Zoerle Tommaso , Robba Chiara 
Published: January 2024

Abstract
Background
The early management of polytrauma patients with traumatic spinal cord injury (tSCI) is a major challenge. Sparse data is available to provide optimal care in this scenario and worldwide variability in clinical practice has been documented in recent studies.

Methods
A multidisciplinary consensus panel of physicians selected for their established clinical and scientific expertise in the acute management of tSCI polytrauma patients with different specializations was established. The World Society of Emergency Surgery (WSES) and the European Association of Neurosurgical Societies (EANS) endorsed the consensus, and a modified Delphi approach was adopted.

Results
A total of 17 statements were proposed and discussed. A consensus was reached generating 17 recommendations (16 strong and 1 weak).

Conclusions
This consensus provides practical recommendations to support a clinician’s decision making in the management of tSCI polytrauma patients.
Interpreting the efficacy enhancement mechanism of Chinese medicine processing from a biopharmaceutic perspective
Authors: Yang Bing , Zhang Zhubin , Song Jinjing , Qi Tianhao , Zeng Jingqi , Feng Liang , Jia Xiaobin 
Published: January 2024

AbstractChinese medicine processing (CMP) is a unique pharmaceutical technology that distinguishes it from natural medicines. Current research primarily focuses on changes in chemical components to understand the mechanisms behind efficacy enhancement in processing. However, this paper presents a novel perspective on the biopharmaceutics of CMP. It provides a comprehensive overview of the current research, emphasizing two crucial aspects: the role of ‘heat’ during processing and the utilization of processing adjuvants. The paper highlights the generation of easily absorbed components through the hydrolysis of glycosides by ‘heat’, as well as the facilitation of dissolution, absorption, and targeted distribution of active components through the utilization of processing adjuvants. From a biopharmaceutic perspective, this paper provides a lucid comprehension of the scientific foundation for augmenting the efficacy of CMP. Moreover, it proposes a three-dimensional research framework encompassing chemical reactions, phase transitions, and biopharmaceutical properties to further investigate the mechanisms involved in enhancing the efficacy of CMP.
Graphical Abstract
A position statement on the post gene-therapy rehabilitation of aromatic I-amino acid decarboxylase deficiency patients
Authors: Lee Hui-Min , Mercimek-Andrews Saadet , Horvath Gabriella , Marchese Diana , Poulin Richard E. , Krolick Alexis , Tierney Kati-Lyn , Turna Jasmine , Wei Judy , Hwu Wuh-Liang 
Published: January 2024

AbstractAromatic l-amino acid decarboxylase (AADC) deficiency is a rare genetic disorder of monoamine neurotransmitter synthesis that presents with a range of symptoms, including motor dysfunction and limited attainment of developmental motor milestones. The approval of eladocagene exuparvovec, a gene therapy for AADC deficiency with demonstrated efficacy for motor improvements, now expands the range of motor outcomes possible for patients with this disorder. However, recommendations and guidelines for therapy following treatment with gene therapy are lacking. To ensure patients can reach their full potential following treatment with gene therapy, it is essential they receive rehabilitation therapies designed specifically with their impairments and goals in mind. Therefore, we highlight specific rehabilitative needs of patients following gene therapy and propose a set of recommendations for the post-treatment period based on collective experiences of therapists, physicians, and caregivers treating and caring for patients with AADC deficiency who have been treated with gene therapy. These recommendations include a focus on periods of intensive therapy, facilitating active movements, training for functional abilities, cognitive and communication training, parent/caregiver empowerment, collaboration between therapists and caregivers to develop in-home programs, and the incorporation of supplemental forms of therapy that patients and their families may find more enjoyable and engaging. Many of these rehabilitative strategies may be employed prior to gene therapy. However, these recommendations will be valuable for therapists, caregivers, and wider treatment teams as they prepare for the post-treatment journey with these patients. Furthermore, the considerations and recommendations presented here may prove beneficial outside the AADC deficiency community as gene therapies and other treatments are developed and approved for other rare diseases.
c-MET-positive circulating tumor cells and cell-free DNA as independent prognostic factors in hormone receptor-positive/HER2-negative metastatic breast cancer
Authors: Park Jieun , Chang Eun Sol , Kim Ji-Yeon , Chelakkot Chaithanya , Sung Minjung , Song Ji-Young , Jung Kyungsoo , Lee Ji Hye , Choi Jun Young , Kim Na Young , Lee Hyegyeong , Kang Mi-Ran , Kwon Mi Jeong , Shin Young Kee , Park Yeon Hee , Choi Yoon-La 
Published: January 2024

Abstract
Background
Endocrine therapy resistance in hormone receptor-positive/HER2-negative (HR+/HER2−) breast cancer (BC) is a significant clinical challenge that poses several unmet needs in the management of the disease. This study aimed to investigate the prognostic value of c-MET-positive circulating tumor cells (cMET+ CTCs), ESR1/PIK3CA mutations, and cell-free DNA (cfDNA) concentrations in patients with hormone receptor-positive (HR+) metastatic breast cancer (mBC).

Methods
Ninety-seven patients with HR+ mBC were prospectively enrolled during standard treatment at Samsung Medical Center. CTCs were isolated from blood using GenoCTC® and EpCAM or c-MET CTC isolation kits. PIK3CA and ESR1 hotspot mutations were analyzed using droplet digital PCR. CfDNA concentrations were calculated using internal control copies from the ESR1 mutation test. Immunocytochemistry was performed to compare c-MET overexpression between primary and metastatic sites.

Results
The proportion of c-MET overexpression was significantly higher in metastatic sites than in primary sites (p = 0.00002). Survival analysis showed that c-MET+ CTC, cfDNA concentration, and ESR1 mutations were significantly associated with poor prognosis (p = 0.0026, 0.0021, and 0.0064, respectively) in HR+/HER2− mBC. By contrast, EpCAM-positive CTC (EpCAM+ CTC) and PIK3CA mutations were not associated with progression-free survival (PFS) in HR+/HER2− mBC. Multivariate analyses revealed that c-MET+ CTCs and cfDNA concentration were independent predictors of PFS in HR+/HER2− mBC.

Conclusions
Monitoring c-MET+ CTC, rather than assessing c-MET expression in the primary BC site, could provide valuable information for predicting disease progression, as c-MET expression can change during treatment. The c-MET+ CTC count and cfDNA concentration could provide complementary information on disease progression in HR+ /HER2− mBC, highlighting the importance of integrated liquid biopsy.
Effect of water salinity on immature performance and lifespan of adult Asian tiger mosquito
Authors: Blanco-Sierra Laura , Savvidou Eleni C. , Mpakovasili Evangelia D. , Ioannou Charalampos S. , Bartumeus Frederic , Papadopoulos Nikos T. 
Published: January 2024

Abstract
Background
Aedes albopictus (Skuse, 1894) is a vector for pathogens like dengue, chikungunya, and Zika viruses. Its adaptive capacity enables reproduction in temperate climates and development mainly in artificial containers with fresh water in urbanized areas. Nevertheless, breeding in coastal areas may also occur along with its aggressive invasiveness. Global warming and the consequent rise in sea levels will increase saline (> 30 ppt) or brackish (0.5–30 ppt salt) water in coastal regions. To address whether Ae. albopictus can breed in brackish water, we initiated the current study that analyses the survival of immature stages at different salinity concentrations and explores whether carryover effects occur in the resulting adults. This possible adaptation is important when considering the potential for development in new habitats and expansion of one of the world’s most invasive species.

Methods
We investigated the influence of salinity on the survival of Ae. albopictus larvae and adults under laboratory-controlled conditions. First instar larvae were exposed to different salinity concentrations (0 to 30 ppt) and their development time, pupation, adult emergence, and overall survival were monitored daily. We used Kaplan-Meier and Cox regression models to analyze the survival rates at different salinity levels. Furthermore, life tables were constructed under each salinity concentration.

Results
Increasing salt concentrations significantly increased the mortality risk during immature development, while no significant effect was observed on adult mortality risk. A comparison between distilled and bottled water revealed a notable increase in overall mortality risk for individuals developing in distilled water. However, no significant effects were found when analyzing survival from the first larval stage to adult emergence and adult lifespan. The life expectancy of immature stages decreased with increasing salt concentrations, although salinity concentration did not significantly impact adult life expectancy.

Conclusions
Our findings suggest that Ae. albopictus, previously considered freshwater species, can successfully develop and survive in brackish waters, even in the absence of characteristic structures found in euryhaline species. These adaptations may enable Ae. albopictus to establish new breeding sites and colonize unexplored territories. Knowledge of these physiological adaptations of Ae. albopictus to salinity should be pursued to increase the range of control of the species, and to make more accurate predictions of its dispersal and vectoring ability.

Graphical Abstract
Optimizing spectrum efficiency in 6G multi-UAV networks through source correlation exploitation
Authors: Alnakhli Mohammad 
Published: January 2024

AbstractIn the context of 6G and 5G communication networks, particularly in disaster-stricken areas with a surging demand for connectivity and the rapid evolution of the internet of everything (IoE), the imperative for augmenting spectrum efficiency in unmanned aerial vehicles (UAVs) communication-enabled wireless networks becomes ever more pronounced. This work embarks on the mission of significantly enhancing spectrum efficiency by capitalizing on redundancies, such as correlations inherent in data sources like co-located video sensors. While the intuitive potential of exploiting redundancies for spectrum efficiency enhancement is apparent, our approach is systematically structured. We introduce a linear programming framework designed to meticulously allocate bandwidth to individual links based on their respective demands, while judiciously adhering to spatial spectrum reuse constraints. Subsequently, we employ this linear program to empirically quantify the improvements in spectrum efficiency engendered by source correlations. Furthermore, we elucidate various strategies for harnessing these correlations to maximize spectrum efficiency gains, while navigating the trade-off terrain between computational complexity and precision. Our findings resoundingly underscore the trans-formative power of identifying the precise set of source correlations, resulting in spectrum efficiency enhancements of up to two orders of magnitude. In terms of network performance, the judicious exploitation of source correlations grants admission to nearly 100% of delay-intolerant traffic, alongside substantial reductions in mean delay for delay-tolerant traffic.”
Assessment of different continence definitions in the context of the randomized multicenter prospective LAP-01 trial—Does the best definition change over time?
Authors: Holze Sigrun , Kuntze Anna Sophie , Mende Meinhard , Neuhaus Petra , Truss Michael C. , Do Hoang Minh , Dietel Anja , Franz Toni , Stolzenburg Jens-Uwe 
Published: January 2024

Abstract
Background
A uniform definition of continence is urgently needed to allow the comparison of study results and to estimate patient outcomes after radical prostatectomy (RP). To identify a practical definition that includes both objective and subjective aspects in a tangible way, we assessed different continence definitions and evaluated which best reflects the patients’ subjective perception of continence.

Methods
Our analyses included 718 patients that underwent either robot-assisted radical prostatectomy (RARP) or laparoscopic radical prostatectomy (LRP) in a multicenter randomized patient-blinded trial. Continence was assessed through patient questionnaires prior to and at 3, 6 and 12 months after surgery which included the number of pads used per day, the ICIQ-SF and the question “Do you suffer from incontinence? (yes/no)” to assess subjective continence. We used Krippendorff’s Alpha to calculate the agreement of different continence definitions with the subjective perception.

Results
At 3 months, the “0/safety pad” definition shows the highest agreement by alpha = 0.70 (vs. 0.63 for “0 pads” and 0.37 for “0–1 pad”). At 6 and 12 months “0 pads” is the better match, with alpha values of 0.69 (vs. 0.62 and 0.31) after 6 months and 0.70 (vs. 0.65 and 0.32) after 12 months. The ICIQ-SF score shows good correlation with the subjective continence at 3 months (alpha = − 0.79), the coefficient then decreasing to − 0.69 and − 0.59 at 6 and 12 months.

Conclusion
The best continence definition according to the patients’ perspective changes over time, “0 pads” being the superior criterion in the long-term. We recommend using the 0-pad definition for standardized continence reporting, as it is simple yet as accurate as possible given the inevitably high subjectivity of continence perception.
Trial registration The LAP-01 trial was registered with the U.S. National Library of Medicine clinical trial registry (clinicaltrials.gov), NCT number: NCT03682146, and with the German Clinical Trial registry (Deutsches Register Klinischer Studien), DRKS ID number: DRKS00007138