bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear isoform of RAPH1 interacts with FOXQ1 to promote aggressiveness and radioresistance in breast cancer
Authors: Liu Qun , Cao Yu , Wei Xiaolin , Dong Huiting , Cui Mengyao , Guan Shu , Liu Bo , Wang Xu , Xing Peng 
Published: December 2023

AbstractRadioresistance limits the efficacy of radiotherapy against breast cancer, especially the most lethal subtype of breast cancer, triple-negative breast cancer (TNBC). Epithelial-to-mesenchymal transition (EMT) is closely related to tumor radioresistance. In this work, we attempted to identify the key EMT-related transcription factor(s) that can induce radioresistance in breast cancer cells. A set of 44 EMT transcription factors were analyzed in parental and radioresistant TNBC cell lines. The function of FOXQ1, a differentially expressed transcription factor, was determined in TNBC radioresistance. FOXQ1-interacting proteins were identified by co-immunoprecipitation and mass spectrometry. Compared with parental cells, FOXQ1 was significantly upregulated in radioresistant TNBC cells. Silencing of FOXQ1 increased the radiosensitiviy of radioresistant TNBC cells both in vitro and in vivo. FOXQ1 associated with a nuclear isoform of RAPH1 (named RAPH1-i3) in radioresistant TNBC cells. Overexpression of RAPH1-i3 enhanced TNBC cell proliferation and migration, and most interestingly, induced radioresistance in parental TNBC cells when co-expressed with FOXQ1. Similar findings were observed in estrogen receptor-positive breast cancer cell lines that had co-expression of RAPH1-i3 and FOXQ1. Mechanistically, co-expression of RAPH1-i3 and FOXQ1 activated STAT3 signaling and increased the expression of CCND1, MCL1, Bcl-XL, and MMP2. Depletion of RAPH1-i3 impaired the radioresistance of radioresistant TNBC cells. Additionally, RAPH1-i3 upregulation was associated with advanced tumor stage and reduced disease-free survival in TNBC patients. These results collectively show that RAPH1-i3 interacts with FOXQ1 to promote breast cancer progression and radioresistance. RAPH1-i3 and FOXQ1 represent therapeutic targets for the treatment of breast cancer including TNBC.
Long-term memory of experienced jays facilitates problem-solving by naïve group members in the wild
Authors: Jo Hyein , McCune Kelsey B. , Jablonski Piotr G. , Lee Sang‑im 
Published: December 2023

AbstractLong-term memory affects animal fitness, especially in social species. In these species, the memory of group members facilitates the acquisition of novel foraging skills through social learning when naïve individuals observe and imitate the successful foraging behavior. Long-term memory and social learning also provide the framework for cultural behavior, a trait found in humans but very few other animal species. In birds, little is known about the duration of long-term memories for complex foraging skills, or the impact of long-term memory on group members. We tested whether wild jays remembered a complex foraging task more than 3 years after their initial experience and quantified the effect of this memory on naïve jay behavior. Experienced jays remembered how to solve the task and their behavior had significant positive effects on interactions by naïve group members at the task. This suggests that natural selection may favor long-term memory of solutions to foraging problems to facilitate the persistence of foraging skills that are specifically useful in the local environment in social birds with long lifespans and overlapping generations.
Impact of hydrocolloid dressings in the prevention of pressure ulcers in high-risk patients: a randomized controlled trial (PENFUP)
Authors: Cortés Olga L. , Herrera Victor M. , Salazar Luz D. , Rojas Yudy A. , Esparza Maribel , Taborda Alejandra , Dennis Rodolfo José 
Published: December 2023

AbstractIt is uncertain whether hydrocolloid dressings, a more costly intervention than offering standard care with petrolatum, is superior to prevent pressure ulcers among hospitalized high-risk adults. Randomized, parallel-group, open-label, superiority trial with an active control group, blinded for investigators, event validators, and analysts (December 1, 2015 to December 12, 2017). Eligible patients were ≥ 18 years of age with intact skin judged as high-risk for skin ulcers (Braden scale), admitted to surgical or medical wards of two tertiary-level hospitals. Participants were randomized (1:1) to protection with hydrocolloid dressings or petrolatum. The primary outcome was the first occurrence of pressure ulcers (with post-injury photographs adjudicated by three judges) under intention-to-treat analysis. Based on prior cost analysis, and the available resources (assumed incidence of 6 ulcers/1000 patient-days in controls), inclusion of up to 1500 participants allowed to surpass a one-sided superiority threshold > 5% based on a target efficacy > 40% for dressings. We planned an economic analysis using a decision tree model based on the effectiveness of the study results from a perspective of the third payer of health care. After inclusion of 689 patients (69 events), the trial was stopped for futility after a planned interim analysis (conditional power < 0.1 for all scenarios if the trial was completed). Pressure ulcers had occurred in 34 (10.2%) patients in the intervention group [9.6 per 1000 patient-days] and 35 (9.9%) participants in the control group [7.9 per 1000 patient-days], HR = 1.07 [95% CI 0.67 to 1.71]. The estimated incremental cost for dressings (a dominated strategy) was USD 52.11 per patient. Using hydrocolloid dressings was found similar to petrolatum for preventing pressure ulcers among hospitalized high-risk patients. As it conveys additional costs, and in this study was unlikely to demonstrate enough superiority, this strategy did not overcome conventional skin care.Trial registration: ClinicalTrials.gov identifier (NCT number): NCT02565745 registered on December 1, 2015.
Conception and pilot testing of a self-management health application for patients with pollen-related allergic rhinitis and allergic asthma-the APOLLO app
Authors: Landesberger V. , Grenzebach K. , Schreiber F. , Nowak D. , Gröger M. , Oppel E. , Schaub B. , French L. E. , Kutzora S. , Quartucci C. , Herr C. , Heinze S. 
Published: December 2023

AbstractIt has been shown that pollen information services are an important self-management tool for patients with pollen-related allergic rhinitis (AR) and allergic asthma (AA). This study aimed to design an online application for patients with AR and AA, which supports patients to better manage their disease as well as to evaluate the app and present the first results of the pilot study. The pollen data were obtained from the electronic pollen information network of Bavaria, Germany. Participants were asked to fill in their allergy-related complaints in the app over a 60-day period. Subsequently, the app was evaluated. Indices and diagrams visualized the participants’ individual complaints as well as the daily pollen concentration in the air. In order to motivate participants to complete the app on a daily basis, we used elements of gamification. Two thirds of the participants (N = 46) reported feeling better informed about pollen counts and their allergy when using the app. The app's simple and comprehensible design was rated positively. More than 80% of the participants would recommend the app to their family and friends. The app can be a tool for patients with AR and AA to better understand their disease.
Downregulated NPAS4 in multiple brain regions is associated with major depressive disorder
Authors: Selçuk Berkay , Aksu Tuana , Dereli Onur , Adebali Ogün 
Published: December 2023

AbstractMajor Depressive Disorder (MDD) is a commonly observed psychiatric disorder that affects more than 2% of the world population with a rising trend. However, disease-associated pathways and biomarkers are yet to be fully comprehended. In this study, we analyzed previously generated RNA-seq data across seven different brain regions from three distinct studies to identify differentially and co-expressed genes for patients with MDD. Differential gene expression (DGE) analysis revealed that NPAS4 is the only gene downregulated in three different brain regions. Furthermore, co-expressing gene modules responsible for glutamatergic signaling are negatively enriched in these regions. We used the results of both DGE and co-expression analyses to construct a novel MDD-associated pathway. In our model, we propose that disruption in glutamatergic signaling-related pathways might be associated with the downregulation of NPAS4 and many other immediate-early genes (IEGs) that control synaptic plasticity. In addition to DGE analysis, we identified the relative importance of KEGG pathways in discriminating MDD phenotype using a machine learning-based approach. We anticipate that our study will open doors to developing better therapeutic approaches targeting glutamatergic receptors in the treatment of MDD.
Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis
Authors: Diotallevi Aurora , Buffi Gloria , Barocci Simone , Ceccarelli Marcello , Bencardino Daniela , Andreoni Francesca , Orlandi Chiara , Ferri Marilisa , Vandini Daniela , Menzo Stefano , Carlotti Eugenio , Casabianca Anna , Magnani Mauro , Galluzzi Luca 
Published: December 2023

AbstractThe current global pandemic of COVID-19 is characterized by waves of infection due to the emergence of new SARS-CoV-2 variants carrying mutations on the Spike (S) protein gene. Since autumn 2020 many Variants of Concern (VOC) have been reported: Alpha/B.1.1.7, Beta/B.1.351, Gamma/P.1, Delta/B.1.617.2, Omicron/B.1.1.529, and sublineages. Surveillance of genomic variants is currently based on whole-genome sequencing (WGS) of viral genomes on a random fraction of samples positive to molecular tests. WGS involves high costs, extended analysis time, specialized staff, and expensive instruments compared to a PCR-based test. To rapidly identify the VOCs in positive samples, six assays based on real-time PCR and high-resolution melting (HRM) were designed on the S gene and applied to 120 oro/nasopharyngeal swab samples collected from October 2020 to June 2022 (106 positive and 14 negative samples). Overall, the assays showed 100% specificity and sensitivity compared with commercial PCR tests for COVID-19. Moreover, 104 samples out of 106 (98.1%) were correctly identified as follows: 8 Wuhan (wild type), 12 Alpha, 23 Delta, 46 Omicron BA.1/BA.1.1, 15 Omicron BA.2/BA.4/BA.5. With our lab equipment, about 10 samples can be processed every 3 h at the cost of less than € 10 ($ 10.60) per sample, including RNA extraction. The implementation of this approach could help local epidemiological surveillance and clinical decision-making.
Machine learning‐based approach for maritime target classification and anomaly detection using millimetre wave radar Doppler signatures
Authors: Rahman Samiur , Vattulainen Aleksanteri B. , Robertson Duncan A. 
Published: December 2023

AbstractThe authors present multiple machine learning‐based methods for distinguishing maritime targets from sea clutter. The main goal for this classification framework is to aid future millimetre wave radar system design for marine autonomy. Availability of empirical data at this frequency range in the literature is scarce. The classification and anomaly detection techniques reported here use experimental data collected from three different field trials from three different millimetre wave radars. Two W‐band radars operating at 77 and 94 GHz and a G‐band radar operating at 207 GHz were used for the field trial data collection. The dataset encompasses eight classes including sea clutter returns. The other targets are boat, stand up paddleboard/kayak, swimmer, buoy, pallet, stationary solid object (i.e. rock) and sea lion. The Doppler signatures of the targets have been investigated to generate feature values. Five feature values have been extracted from Doppler spectra and four feature values from Doppler spectrograms. The features were trained on a supervised learning model for classification as well as an unsupervised model for anomaly detection. The supervised learning was performed for both multi‐class and 2‐class (sea clutter and target) classification. The classification based on spectrum features provided an 84.3% and 80.1% validation and test accuracy respectively for the multi‐class classification. For the spectrogram feature‐based learning, the validation and test accuracy for multi‐class increased to 93.3% and 88.7% respectively. For the 2‐class classification, the spectrum feature‐based training accuracies are 88.1% and 86.8%, whereas with the spectrogram feature‐based model, the values are 95% and 94.1% for validation and test accuracies respectively. A one class support vector machine was also applied to an unlabelled dataset for anomaly detection training, with 10% outlier data. The cross‐validation accuracy has shown very good agreement with the expected anomaly detection rate.
Testing the spatial geometry of the Universe with TianQin: Prospect of using supermassive black hole binaries
Authors: Pan Yu , Diao Jingwang , Qi Jing-Zhao , Li Jin , Cao Shuo , Jiang Qing-Quan 
Published: December 2023

Context. The determination of the spatial geometry of the Universe plays an important role in modern cosmology. Any deviation from the cosmic curvature ΩK = 0 would have a profound impact on the primordial inflation paradigm and fundamental physics.
Aims. In this paper, we carry out a systematic study of the prospect of measuring the cosmic curvature with the inspiral signal of supermassive black hole binaries (SMBHBs) that could be detected with TianQin.
Methods. The study is based on a method that is independent of cosmological models. It extended the application of gravitational wave (GW) standard sirens in cosmology. By comparing the distances from future simulated GW events and simulated H(z) data, we evaluated whether TianQin produced robust constraints on the cosmic curvature parameter Ωk. More specifically, we considered three-year to ten-year observations of supermassive black hole binaries with total masses ranging from 103 M⊙ to 107 M⊙.
Results. Our results show that in the future, with the synergy of ten-year high-quality observations, we can tightly constrain the curvature parameter at the level of 1σ Ωk = −0.002 ± 0.061. Moreover, our findings indicate that the total mass of SMBHB does influence the estimation of cosmic curvature, as implied by the analysis performed on different subsamples of gravitational wave data.
Conclusions. Therefore, TianQin is expected to provide a more powerful and competitive probe of the spatial geometry of the Universe, compared to future spaced-based detectors such as DECIGO.
Die Anwendung der Salzburg-Kriterien in der Diagnose des
non-konvulsiven Status epilepticus
Authors: Willems Laurent Maximilian , Rosenow Felix , Strzelczyk Adam 
Published: December 2023

ZusammenfassungAufgrund oftmals nur subtiler motorischer Phänomene stellt die Diagnose
des nicht-konvulsiven Status epilepticus (non-convulsive status epilepticus,
NCSE) eine häufige Differentialdiagnose bei Patientinnen und Patienten
mit Vigilanzminderung dar. Da eine frühe adäquate Therapie des
NCSE mit einem besseren Therapieansprechen sowie funktionellen Outcome
einhergeht, ist eine sichere und frühe diagnostische Sicherung von hoher
Relevanz. Aufbauend auf den Ergebnissen des „London-Innsbruck Coloquium
on Status Epilepticus and Acute Seizures“, welches 2013 in Salzburg
stattfand, wurde von einer Expertengruppe ein Diagnosealgorithmus basierend auf
elektroenzephalographischen und klinischen Kriterien entwickelt, der als
„Salzburg-Kriterien“ bekannt ist. Über die letzten Jahre
wurden diese Kriterien validiert und klinisch erprobt, teilweise aber auch
hinsichtlich ihrer Anwendbarkeit kritisiert. Dieser Artikel erläutert
die Salzburg-Kriterien und diskutiert potenzielle Fallstricke in der
Anwendung.
Radioembolisation primärer und sekundärer Lebermalignome mit Holmium-166-Mikrosphären – eine kurze Übersicht
Authors: Zacherl Mathias Johannes , Todica Andrei , Ilhan Harun 
Published: December 2023

ZusammenfassungIm Rahmen der transarterielle Radioembolisation (TARE) werden überwiegend 90Y-markierten Glas- und Harzmikrosphären verwendet. Seit Kurzem sind mit 166Ho neue Mikrosphären auf Basis von Poly-L-Milchsäure verfügbar, welche auch in Deutschland zunehmend in der klinischen Routine eingesetzt werden. Aufgrund physikalischer Unterschiede bieten 166Ho-Mikrosphären dabei spezielle Vorteile im Vergleich zur TARE mit 90Y-markierten Mikrosphären. In diesem Artikel soll ein kurzer Überblick über die TARE mit 166Ho-Mikrosphären gegeben werden.
Die Zahntransplantation – eine Übersicht unter
Berücksichtigung des Lückenmanagements
Authors: Khoury Fouad , Khoury Chantal , Wildenhof Jan 
Published: December 2023

ZusammenfassungDie Zahntransplantation ist eine sehr alte Therapieform. Während die
allogene Transplantation von Zähnen aufgrund klassischer immunologischer
Abstoßungsreaktionen für den Praktiker eher bedeutungslos ist,
stellt die autogene Zahntransplantation aufgrund präziser biologischer
und patho-/physiologischer Kenntnisse heute eine sehr sichere
Therapievariante dar. Sie ist eine gute und praktikable Alternative zum
kieferorthopädischen Lückenschluss, zur konventionellen
prothetischen Versorgung und zu Implantationen. Gerade beim jungen Patienten
liegt die Langzeiterfolgsquote bei über 95%. Voraussetzung
dafür sind ein atraumatisches chirurgisches Vorgehen sowie
regelmäßige klinische und röntgenologische Kontrollen,
zumindest in den ersten zwei postoperativen Jahren. Dadurch lassen sich
mögliche entzündliche Komplikationen frühzeitig erkennen
und erfolgreich therapieren.
Maternal Factors and Placental Pathologies Associated with a Diagnosis of Chronic Villitis
Authors: Rose Sherrill J. , Nguyen Eliza M. , Branda Megan E. , Melo Valeria , Vega Beatriz , Kazemi-Butterfield Nazanin , Cheek-Norgan E. Heidi , Girard Sylvie , Norgan Andrew P. , Theiler Regan N. , Enninga Elizabeth Ann L. 
Published: December 2023

Objective To evaluate maternal risk factors associated with chronic villitis of unknown etiology (VUE) and to describe cooccurring placental pathologies.
Study Design A retrospective case–control study was conducted using placental pathology records from deliveries ≥ 20 weeks between 2010 and 2018. Cases were placentas with documented chronic villitis without infectious cause, hereafter called VUE. Controls were placentas without this diagnosis, matched to the cases 2:1. Maternal and neonatal demographic and clinical data were collected. Descriptive statistics are reported with Fisher's exact test or a chi-squared test, as appropriate, and multivariable conditional logistic regression was conducted.
Results Our study included 352 cases with VUE and 657 controls. A diagnosis of gestational diabetes (p = 0.03) and gestational hypertension (p = 0.06) was 1.5 times more likely to occur in those with a VUE diagnosis. A trend was also seen for chronic hypertension (odds ratio [OR] = 1.7, p = 0.07) and preeclampsia (OR = 1.5, p = 0.09) compared with controls. Placentas with VUE, specifically high-grade VUE, were more likely to be small for gestational age (p = 0.01), and to be diagnosed with other placental findings including lymphoplasmacytic or chronic deciduitis (p < 0.01), maternal (p < 0.01) and fetal vascular malperfusion (p = 0.02), and chorionitis (acute or chronic; p < 0.01).
Conclusion Gestational diabetes and hypertension were associated with a diagnosis of VUE, and overall, VUE placentas have more abnormal placental findings compared with control. Understanding VUE risk factors may facilitate prenatal care strategies and counseling to achieve the best outcomes for pregnant patients and their neonates.
Key Points
Novel Two-Step Synthesis of N-Alkylated 2,3-Diaryl-4-quinolones
Authors: Aksenov Alexander V. , Arutiunov Nikolai A. , Zatsepilina Anna M. , Aksenova Anna A. , Aleksandrova Elena V. , Aksenov Nicolai A. , Leontiev Alexander V. , Aksenov Dmitrii A. 
Published: December 2023

AbstractA library of 18 polysubstituted 4-quinolones was conveniently prepared via simple and practical protocol involving N-alkylation of 2-(3-oxoindolin-2-yl)acetonitriles and following NaH-induced ring expansion. The current two-step approach provides feasible access to a subclass of N-protected C3-aryl-substituted 2-phenyl-4-quinolones starting with or just one step away from commercially available 2-arylindoles and nitrostyrenes.
MiniMelt: An instrument for real-time tracking of electron beam additive manufacturing using synchrotron x-ray techniques
Authors: König Hans-Henrik , Semjatov Nick , Spartacus Gabriel , Bidola Pidassa , Ioannidou Chrysoula , Ye Jihui , Renner Jakob , Lienert Ulrich , Faria Guilherme Abreu , Wahlmann Benjamin , Wildheim Martin , Ackelid Ulf , Beckmann Felix , Staron Peter , Hedström Peter , Körner Carolin , Lindwall Greta 
Published: December 2023

The development of a sample environment for in situ x-ray characterization during metal Electron Beam Powder Bed Fusion (PBF-EB), called MiniMelt, is presented. The design considerations, the features of the equipment, and its implementation at the synchrotron facility PETRA III at Deutsches Elektronen-Synchrotron, Hamburg, Germany, are described. The equipment is based on the commercially available Freemelt ONE PBF-EB system but has been customized with a unique process chamber to enable real-time synchrotron measurements during the additive manufacturing process. Furthermore, a new unconfined powder bed design to replicate the conditions of the full-scale PBF-EB process is introduced. The first radiography (15 kHz) and diffraction (1 kHz) measurements of PBF-EB with a hot-work tool steel and a Ni-base superalloy, as well as bulk metal melting with the CMSX-4 alloy, using the sample environment are presented. MiniMelt enables time-resolved investigations of the dynamic phenomena taking place during multi-layer PBF-EB, facilitating process understanding and development of advanced process strategies and materials for PBF-EB.
Dorper sheep in Africa: A review of their use and performance in different environments
Authors: Ojango Julie M.K. , Okpeku Moses , Osei-Amponsah Richard , Kugonza Donald R. , Mwai Okeyo , Changunda Mizeck G.G. , Olori Victor E. 
Published: December 2023

Abstract
The Dorper breed developed in South Africa is used either as a pure breed or crossbred with existing indigenous breeds by many countries across the African continent to improve sheep production. This article presents documented information on the adoption, use, and performance of Dorper sheep across the continent of Africa and opportunities for their more sustainable production under the changing climatic conditions in Africa. Apart from the well-documented information on the Dorper sheep in South Africa, published information on the performance of the sheep is mainly from Eastern Africa. Most countries initially retained purebred Dorpers in nationally owned institutions for multiplication and crossbreeding trials with different indigenous breeds prior to distributing the crossbreds to diverse livestock keepers. The offspring produced through crossbreeding programs with the Dorper have better growth rates than indigenous breeds in the different countries; however, the performance of Dorper sheep in South Africa has not been achieved in any of the other countries. Genomic studies including Dorper sheep have identified regions of interest for resistance to brucellosis and Mycoplasma ovipneumonia that imply adaptability to challenging environments within Dorper sheep. Unfortunately, limitations in systems for guided breeding and monitoring of sheep productivity in Africa have resulted in haphazard crossbreeding of the Dorper. Targeted efforts are required across the different countries to develop breeding programs for improving locally adapted Dorper sheep populations and their crosses with indigenous breeds. New science and technologies need to be innovatively packaged and used to identify and propagate more productive and resilient Dorper and Dorper-based breed-types for the increasingly challenging tropical African range environments.