bluuebunny/crossref_metadata_2025_split huggingface dataset:
Follow‐up of humoral immune response after HPV vaccination using first‐void urine: A longitudinal cohort study
Authors: Téblick Laura , Pattyn Jade , Van Keer Severien , De Smet Annemie , De Coster Ilse , Tjalma Wiebren A. A. , Rajbhandari Ira , Panicker Gitika , Unger Elizabeth R. , Vorsters Alex 
Published: October 2023

AbstractAssessment of humoral immune responses following human papillomavirus (HPV) vaccination currently relies on invasive blood sampling. This longitudinal cohort study explores the usability of first‐void urine as a noninvasive alternative sample for antibody detection. In this study, 58 women receiving three doses of the 9vHPV vaccine within a Gardasil9 (9vHPV) Phase III randomized controlled trial were included. Participants provided paired first‐void urine and blood samples before vaccination (M0), 1 month after the third dose (M7), and ~3 years after the third dose (M43). Type‐specific antibody responses to the 9vHPV types were analyzed in 174 first‐void urine and 172 serum samples using a virus‐like particle‐based IgG multiplex enzyme‐linked immunosorbent assay. Additionally, total human IgG concentrations were determined using the BioPlex assay. At M7, 1 month after complete 9vHPV vaccination, 95%–100% of first‐void urine and 100% of serum samples had detectable concentrations, varying by HPV type. At M43, 84%–100% of first‐void urine and 98%–100% of serum samples had HPV‐specific antibody concentrations. Results show significant Spearman rank correlations between type‐specific HPV‐antibody concentrations for paired first‐void urine and serum at all time points. This study confirms the potential feasibility of utilizing first‐void urine as a noninvasive immunological sample within HPV vaccine trials.
Comprehensive evaluation of the effect of inactivated SARS‐CoV‐2 vaccination on female fertility: A retrospective cohort study
Authors: Ba Zaihua , Yang Aijun , Zhu Shiheng , Li Yuqi , Ma Jiao , Zhang Yingze , Li Zewu , Chen Fei 
Published: October 2023

AbstractFear of possible negative effects of coronavirus disease 2019 (COVID‐19) vaccine on fertility is the main reason for vaccine hesitancy among the public especially women of childbearing age. Despite the high coverage of COVID‐19 vaccination in China, more scientific evidence is still needed to address their concerns and guide fertility counseling and management in the future. Herein, we performed a retrospective cohort study at a single large center for reproductive medicine in China between August 2020 and May 2023. Patients aged 20–42 years with no history of laboratory‐confirmed COVID‐19 were included and categorized into different groups according to their vaccination status. The serum sex hormone levels, anti‐Müllerian hormone concentrations, embryo quality, and pregnancy outcomes were evaluated and compared among them. We found there were no significant differences in the concentrations of follicle‐stimulating hormone, luteinizing hormone and progesterone between the unvaccinated, first‐dose, second‐dose, and booster vaccinated groups. However, the estradiol showed a highly significant increase in the one‐dose vaccinated group compared with its levels in other groups. Among unvaccinated and either vaccinated patients, anti‐Müllerian hormone levels were comparable (p = 0.139). The number of oocytes retrieved, fertilization rate and good‐quality embryo rate were all similar between each group of in vitro fertilization and intracytoplasmic sperm injection. No significant differences were observed regarding other laboratory parameters. Moreover, the vaccination status of infertile couples did not exert any adverse effect on the pregnancy outcomes in all assisted reproductive technologies cycles. In short, we comprehensively evaluated the reproductive safety of inactivated severe acute respiratory syndrome coronavirus 2 vaccine and found any dose of vaccination wouldn't negatively affect female fertility parameters such as sex hormone levels and ovarian reserve. Moreover, this is the first study to complete the live birth follow‐up of the cohort after receiving inactivated severe acute respiratory syndrome coronavirus 2 vaccine, further dispelling the misconception and providing reassurance for decision‐making by clinicians.
Alleviated symptoms of SARS‐CoV‐2 Omicron variant infection in chronic hepatitis B patients with immune control
Authors: Liu Jing , Zhang Huiqing , Kong Jianing , Liu Shiyi , Chen Liping , Jiang Yanming , Wang Jie , Zhang Binbin , Ye Xiaoping , Gong Ling , Zhou Xiang , Chen Gongying , Li Jie , Pan Xiaoben , Zhang Haifeng , Shi Junping 
Published: October 2023

AbstractThe impact of hepatitis B virus (HBV) infection on the progression of coronavirus disease 2019 (COVID‐19) disease remains controversial. We aimed to investigate whether pre‐existing chronic HBV (CHB) infection and therapy with anti‐HBV nucleos(t)ide analogs (NAs) influence the clinical presentation of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) Omicron variant infection. In this study, clinical information was collected via a questionnaire from patients with COVID‐19, and their clinical symptoms were quantitatively assessed for comparative analyses. Additionally, hepatitis B‐related laboratory data were collected for CHB patients. Propensity score matching (PSM) was used to minimize confounding biases. A total of 785 patients with COVID‐19 were included in the cohort, of which 387 were identified as being infected with CHB infection and they were categorized as being in the immune control or clearance phase. After PSM, the CHB group (n = 222) had a shorter duration of fever and disease course, milder clinical symptoms, and lower incidence of pneumonia than the non‐CHB group (n = 222) after Omicron variant infection (p < 0.05). After the adjustment of confounding factors, CHB patients showed a lower risk of prolonged fever, severe clinical symptoms, and pneumonia (p < 0.05). However, there were no statistically significant differences in the clinical symptoms and incidence of pneumonia between CHB patients who received and did not receive NAs, or CHB patients who received tenofovir disoproxil fumarate and entecavir (p > 0.05). In conclusion, our findings suggest that the crosstalk of anti‐HBV immunity may contribute to the alleviated symptoms of SARS‐CoV‐2 Omicron variants infection in the CHB patients, independent of anti‐HBV NA therapy.
Remediation of hydrocarbons and metals by hydrocarbon utilizing Pseudomonas taiwanensis YSA‐17 isolated from soil contaminated with petroleum
Authors: Wani Parvaze A. , Olusebi Yusuff K. , Rilwan Bello A. 
Published: October 2023

AbstractCareless handling of petroleum in petrochemical industries releases toxic hydrocarbons and metals to soil and water. The aim of the present study was to isolate hydrocarbon‐utilizing and metal‐tolerant bacteria. Hydrocarbon‐utilizing bacteria from petroleum‐contaminated soils were isolated on the Bushnell Hass medium. Hydrocarbon degradation by Pseudomonas taiwanensis strain YSA‐17 was observed by gas chromatography–mass spectrometry. Bioaccumulation of metals by strain YSA‐17 was assessed in nutrient broth. Among different strains, YSA‐17 showed the highest potential for hydrocarbon utilization. After 20 days of incubation, YSA‐17 completely degraded one compound and during its degradation, there was the formation of 13 new compounds which were absent in uninoculated control. Results of scanning electron microscope and Fourier transmission infrared (FTIR) indicated degradation of hydrocarbons. FTIR showed the formation of new functional groups in YSA‐17 inoculated medium. Expression of the total quantity of hydrocarbon‐degrading gene (AlkB and NehAc) in petroleum‐amended nutrient broth inoculated with strain YSA‐17 enhanced significantly during 20 days of incubation compared to control. YSA‐17 also significantly removed metals. This study concluded that bioinoculant can be utilized for the bioremediation of pollutants cocontaminated with hydrocarbons and metals. Petroleum‐contaminated soil will be remediated for pollutants.
Metagenomic characterization reveals virus coinfections associated with Newcastle disease virus among poultry in Kenya
Authors: Panyako Philip M. , Ommeh Sheila C. , Kuria Stephen N. , Lichoti Jacqueline K. , Musina Johns , Nair Venugopal , Nene Vish , Oyola Samuel O. , Munir Muhammad 
Published: October 2023

AbstractNewcastle disease (ND) is an endemic viral disease affecting poultry and causing massive economic losses. This cross‐sectional purposive study detected coinfections that are associated with the Newcastle disease virus among poultry from selected regions in Kenya. Cloacal (n = 599) and oral–pharyngeal (n = 435) swab samples were collected and pooled into 17 and 15 samples, respectively. A total of 17,034,948 and 7,751,974 paired‐end reads with an average of 200 nucleotides were generated from the cloacal and oral–pharyngeal swab samples, respectively. Analysis of the de novo assembled contigs identified 177 and 18 cloacal and oral–pharyngeal contigs, respectively with hits to viral sequences, as determined by BLASTx and BLASTn analyses. Several known and unknown representatives of Coronaviridae, Picobirnaviridae, Reoviridae, Retroviridae, and unclassified Deltavirus were identified in the cloacal swab samples. However, no Newcastle disease virus (family Paramyxoviridae) was detected in the cloacal swabs, although they were detected in the oropharyngeal swabs of chickens sampled in Nairobi, Busia, and Trans Nzoia. Additionally, sequences representative of Paramyxoviridae, Coronaviridae, and Retroviridae were identified in the oral–pharyngeal swab samples. Infectious bronchitis virus and rotavirus were chickens' most prevalent coinfections associated with the Newcastle disease virus. The detection of these coinfections suggests that these viruses are significant threats to the control of Newcastle disease as the Newcastle disease virus vaccines are known to fail because of these coinfections. Therefore, this study provides important information that will help improve disease diagnosis and vaccine development for coinfections associated with the Newcastle disease virus.
Antibacterial and antibiofilm efficacy of colistin & meropenem conjugated silver nanoparticles against Escherichia coli and Klebsiella pneumoniae
Authors: Şirin M. Cem , Cezaroğlu Yasemin , Sesli Çetin E. , Arıdoğan Buket , Trak Diğdem , Arslan Yasin 
Published: October 2023

AbstractThe progressive increase in infections caused by multidrug‐resistant (MDR) Gram‐negative bacteria and the emergence of resistance to last‐resort antimicrobial drugs in recent years necessitate the development of new therapeutic strategies. This study was conducted to obtain nanostructured antimicrobials by conjugating colistin (COL) and meropenem (MEM) antibiotics with biosynthesized silver nanoparticles (bio‐AgNPs) via the green synthesis method using Rosa damascena extract, and to investigate the antibacterial and antibiofilm activity of these nanostructures against Escherichia coli and Klebsiella pneumoniae strains. Ultraviolet–visible spectrophotometry, high‐resolution‐transmission electron microscopy, atomic force microscopy, X‐ray diffraction, and Fourier transform‐infrared spectroscopy analyses were performed to determine the physical and chemical properties of synthesized bio‐AgNPs, COL@bio‐AgNPs, MEM@bio‐AgNPs, and COL&MEM@bio‐AgNPs. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of nanoparticles were determined on standard and MDR clinical strains. The antibiofilm efficacy and cytotoxic effect of nanoparticles were evaluated by the crystal violet dye method and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide dye method, respectively. The characterization analyses demonstrated that the synthesized nanoparticles had crystal structure and spherical morphology (5.6–30.2 nm in size). Antibiotic conjugated nanoparticles exhibited better antimicrobial activity and lower MIC values (0.125–4 µg/mL) on the tested strains compared to free antibiotics, and MIC values were decreased up to 1024‐fold (p < 0.05). Antibiotic conjugated nanoparticles were found to be more effective in biofilm eradication than free antibiotics and bio‐AgNPs and had a less inhibitory effect on peripheral blood mononuclear cell viability. The findings revealed that antibiotic‐conjugated nanoparticles have the potential to be used as an effective antimicrobial drug against MDR E. coli and K. pneumoniae strains.
Creativity in Older Adults: Elucidating the Interactions between Cognitive Functions Underlying Creativity and the Role of Demographic Variables
Authors: Alcock Stephanie , Ferreira‐Correia Aline , Cockcroft Kate 
Published: October 2023

ABSTRACTCreativity involves generating novel and valuable ideas. While the importance of creative thinking is widely acknowledged, its cognitive basis is poorly understood, particularly in older adults. This study aimed to develop and test an explanatory model of creative thinking to elucidate its underlying cognitive functions in an elderly sample. The role of demographic variables, including age, multilingualism, socioeconomic status (SES), level of education, and gender in creative thinking was also investigated. One hundred and twenty‐five participants aged 65 years and above‐completed measures of divergent, convergent and associative thinking, as well as task‐switching, inhibition and fluid intelligence. Structural equation modeling (SEM) analyzed the relationships between these cognitive functions, and group differences assessed whether there were significant differences in the cognitive measures based on the demographic variables. Creative thinking appeared to entail the cooperation between divergent and convergent thinking, which both rely on associative thinking, suggesting an associative basis of creative thinking. Creative thinking involved fluid intelligence and task‐switching, but not inhibition, recasting it as a higher‐order function. This study supports the dual‐process account of creative thinking by demonstrating an associative basis and the role of executive functions. Differences among education and SES groups occurred for most cognitive functions analyzed. These findings inform prevailing theoretical frameworks of creative thinking.
Oncoplastic surgery for breast cancer: Global perspectives and trends
Authors: Armstrong Kate , Maxwell Jessica 
Published: October 2023

AbstractOncoplastic surgery (OPS) is a form of breast conservation that combines definitive oncologic resection with optimal aesthetic outcomes. Various definitions exist, with most focusing on volume displacement techniques to close the lumpectomy defect and redistribute the remaining breast volume over the preserved breast. Although its oncologic safety has been well demonstrated, uptake into practice varies globally. This article details the definition, safety, training opportunities, and practice patterns of OPS on a global scale.
The African Research Group for Oncology: A decade fostering colorectal cancer research in Nigeria
Authors: Dare Anna J. , Olatoke Samuel A. , Okereke Chukwuma E. , Abdulkareem Fatimah B. , Adeyeye Ademola , Badejo Olawale , Du Mengmeng , Fayenuwo Oluwaleke J. , Gali Bata M. , Kahn Rivka , Knapp Gregory , Ntiamoah Peter , Olcese Cristina , Oludara Mobolaji A. , Omisore Adeleye , Omoyiola Oluwatosin Z. , Owoade Israel A. , Brennan Murray F. , Kingham T. Peter , Alatise Olusegun I. ,   for the African Research Group for Oncology (ARGO)
Published: October 2023

AbstractThe African Research Group for Oncology (ARGO) was formed in 2013 to undertake methodologically rigorous cancer research in Nigeria, and to strengthen cancer research capacity in the country through training and mentorship of physicians, scientists, and other healthcare workers. Here, we describe how ARGO's work in colorectal cancer (CRC) has evolved over the past decade. This includes the consortium's scientific contributions to the understanding of CRC in Nigeria and globally and its research capacity‐building program.
An update on the global trends in the burden of primary liver cancers
Authors: Khan Asama , Dhir Mashaal 
Published: October 2023

AbstractLiver cancer (LC) remains one of the major causes of cancer‐related mortality worldwide. The Incidence, mortality, and prevalence associated with primary LCs were analyzed over the past decade, using GLOBOCAN 2012 and 2020, to understand the trends related to geographic and socioeconomic factors. While total cases of primary LCs continue to rise, global rates of LC incidence and mortality are slowing, mostly driven by changes seen in historically endemic regions.
Adoption of cytoreductive surgery in the management of peritoneal malignancies—Global trends
Authors: Sedighim Shaina , Khan Aaqil , Li Amy Y. , Tajik Fatemeh , Radhakrishnan Vinodh K. , Eng Oliver , Turaga Kiran , Senthil Maheswari 
Published: October 2023

AbstractCytoreductive surgery (CRS) has now been accepted as an integral component in the management of gastrointestinal and gynecological cancers with peritoneal metastases. Since the adoption of CRS is influenced by access to advanced medical facilities, trained multidisciplinary teams, and funding, there is wide variability in incorporation of CRS into routine clinical practice between high‐ versus low‐ and middle‐income countries. This review highlights the global trends in the adoption of CRS for peritoneal malignancies with a specific focus on the establishment of CRS programs and barriers to incorporate CRS into routine clinical care in low‐ and middle‐income countries.
Recognizing benign and malignant skin lesions: Effect of a dermoscopy training course for general practitioners
Authors: Bruijn Marjolein S. , Visser Els , van der Heide Wouter K. 
Published: October 2023

AbstractBackgroundThe incidence of skin cancer is rapidly increasing, leading to a growing amount of general practitioner (GP) consultations of patients with suspected skin lesions. It is essential that GPs have good training skills to differentiate between benign and malignant skin lesions. Dermoscopy in addition to clinical examination can help them to develop these skills.ObjectivesIn this article, we examine the effect of a 2‐day training course in dermoscopy among Dutch GPs.MethodsThe educational intervention was designed as blended learning and consisted of a 2‐day training course on dermoscopy, clinical features, and management of patients with suspected skin lesions in primary care. Before the training course, all participants were required to take a test (T0). This test was repeatedly administered at the end of the second course day (T1) and within 6 months after completion of the course (T2). Outcomes were the specificity and sensitivity of recognizing skin lesions and the changes between the test moments.ResultsIn total, 400 GPs participated in the training course. At T1, the specificity for detecting benign lesions was higher than at T0 (0.91 vs. 0.75). This specificity remains at the same level at T2, 6 months after the course (0.91 p < 0.001). The sensitivity for detecting malignant lesions stayed about the same level: 0.80 at T0 and 0.84 at T2 (p < 0.001).ConclusionsThe results of our study show a sustaining positive effect of a 2‐day training course on detecting benign skin lesions by GPs.
The gut microbiota metabolite butyrate mitigates MPTP/MPP+‐induced Parkinson's disease by inhibiting the JAK2/STAT3 signaling pathway
Authors: Ji Li‐Li , Huang Ting‐Ting , Mao Lun‐Lin , Xu Yuan‐Feng , Chen Wen‐Ya , Wang Wei‐Wei , Wang Li‐Hui 
Published: October 2023

AbstractButyrate (BU), a gut microbiota‐derived metabolite, has been reported to play a neuroprotective role in Parkinson's disease (PD). However, the specific molecular mechanism of BU has not been fully interpreted. This work aimed to verify the protective effects of BU against MPTP/MPP+‐induced neurotoxicity and explore the mechanisms involved. The results showed that BU protected against MPTP‐induced motor dysfunction and decreased tyrosine hydroxylase (TH) and dopamine transporter (DAT) levels. Additionally, BU pretreatment improved PC12 cell viability and reduced MPP+‐induced PC12 cell apoptosis. BU treatment also attenuated MPP+‐stimulated oxidative stress and inflammatory response in PC12 cells. Furthermore, BU inhibited MPTP/MPP+‐induced hyperactivation of the JAK2/STAT3 signaling in mice and PC12 cells. Besides, a JAK2 agonist, Coumermycin A1 (C‐A1), substantially reversed BU‐mediated inhibition on JAK2/STAT3 phosphorylation in MPP+‐challenged PC12 cells and abated BU‐induced repression on MPP+‐triggered apoptosis, oxidative stress, and inflammatory response in PC12 cells. To sum up, BU might exert neuroprotective effects against MPP+/MPTP‐induced neurotoxicity by inactivating the JAK2/STAT3 signaling.
Photoirradiation‐Gated Excitation‐Dependent Room‐Temperature Phosphorescence in Through‐Space Charge Transfer Molecules
Authors: Li Jiangang , Xing Guohui , Wu Jilong , Zhang Ye , Wei Juan , Liu Shujuan , Ma Yun , Zhao Qiang 
Published: October 2023

AbstractOrganic materials showing reversible changes in persistent room‐temperature phosphorescence (RTP) upon exposure to external stimuli have attracted considerable attention in recent years. One potentially groundbreaking development in this area is the introduction of the gating concept for organic persistent RTP materials. This refers to a material where a desired responsive ability only occurs when it is triggered by a specific stimulus, but it remains a formidable challenge. In this study, photoirradiation‐gated excitation‐dependent (Ex‐De) persistent RTP is achieved. A series of molecules, triphenylphosphine and carbazole derivatives connected through flexible carbon chains, are designed and prepared. Upon removal of ultraviolet (UV) irradiation, all these compounds doped polyvinyl alcohol (PVA) films exhibit obvious blue or green afterglow RTP. Among them, (2‐(9H‐[3,9′‐bicarbazol]−9‐yl)ethyl)triphenylphosphonium bromide (TPP‐2C‐Cz) doped PVA film initially exhibits blue persistent RTP after removal of 300 nm excitation but no RTP upon ceasing 365 nm irradiation. Interestingly, upon UV irradiation with a power density of 0.05 W cm−2 (UVA) for 50 min, the Ex‐De RTP behavior of TPP‐2C‐Cz doped PVA film is activated. Eventually, photoirradiation‐gated Ex‐De afterglow emission will be used for a wide range of applications, including the quantitative determination of UV irradiation dose and the permanent record of UV irradiation history, and read on‐demand.
How gamifying AI shapes customer motivation, engagement, and purchase behavior
Authors: Elmashhara Maher Georges , De Cicco Roberta , Silva Susana C. , Hammerschmidt Maik , Silva Maria Levi 
Published: October 2023

AbstractRecently, firms started to gamify conversational artificial intelligence (AI) agents, such as chatbots, to improve purchase outcomes. This article explores strategies for incorporating gamification into AI systems by investigating the impact of utilitarian and hedonic motivations facilitated by gamified chatbots on various dimensions of customer engagement (cognitive, emotional, and behavioral), as well as the subsequent effects of these dimensions on customers' purchase behavior. By conducting one cross‐sectional and two experimental studies involving real interactions with gamified chatbots, this research identifies two crucial paths that warrant attention: an optimal path from hedonic motivation to behavioral engagement, resulting in enhanced purchase, and a detrimental path from utilitarian motivation to emotional engagement, which reduces purchase. Furthermore, the research compares the effects of two types of gamified chatbots and reveals that a game‐of‐chance‐based chatbot, as opposed to a knowledge‐sharing gamified chatbot, aligns with the optimal path, leading to higher purchasing while at the same time avoiding that customers feel obligated to play the game. Based on these findings, the article provides actionable insights for eliciting favorable psychological and behavioral responses through gamified AI interactions.