bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hederasaponin C inhibits LPS‐induced acute kidney injury in mice by targeting TLR4 and regulating the PIP2/NF‐κB/NLRP3 signaling pathway
Authors: Han Shan , Li Siyuan , Li Jilang , He Jia , Wang Qin‐Qin , Gao Xiang , Yang Shilin , Li Jingjing , Yuan Renyikun , Zhong Guoyue , Gao Hongwei 
Published: October 2023

AbstractAcute kidney injury (AKI) is a common clinical condition associated with increased incidence and mortality rates. Hederasaponin C (HSC) is one of the main active components of Pulsatilla chinensis (Bunge) Regel. HSC possesses various pharmacological activities, including anti‐inflammatory activity. However, the protective effect of HSC against lipopolysaccharide (LPS)‐induced AKI in mice remains unclear. Therefore, we investigated the protective effect of HSC against LPS‐induced renal inflammation and the underlying molecular mechanisms. Herein, using MTT and LDH assays to assess both cell viability and LDH activity; using dual staining techniques to identify different cell death patterns; conducting immunoblotting, QRT‐PCR, and immunofluorescence analyses to evaluate levels of protein and mRNA expression; employing immunoblotting, molecular docking, SPR experiments, and CETSA to investigate the interaction between HSC and TLR4; and studying the anti‐inflammatory effects of HSC in the LPS‐induced AKI. The results indicate that HSC inhibits the expression of TLR4 and the activation of NF‐κB and PIP2 signaling pathways, while simultaneously suppressing the activation of the NLRP3 inflammasome. In animal models, HSC ameliorated LPS‐induced AKI and diminished inflammatory response and the level of renal injury markers. These findings suggest that HSC has potential as a therapeutic agent to mitigate sepsis‐related AKI.
The future opportunities and remaining challenges in the application of nanoparticle‐mediated hyperthermia combined with chemo‐radiotherapy in cancer
Authors: Shirvalilou Sakine , Tavangari Zahed , Parsaei Mohammad Hossein , Sargazi Saman , Sheervalilou Roghayeh , Shirvaliloo Milad , Ghaznavi Habib , Khoei Samideh 
Published: October 2023

AbstractA pivotal cause of death in the modern world, cancer is an insidious pathology that should be diagnosed at an early stage for successful treatment. Development of therapeutic interventions with minimal invasiveness and high efficacy that can discriminate between tumor and normal cells is of particular interest to the clinical science, as they can enhance patient survival. Nanoparticles are an invaluable asset that can be adopted for development of such diagnostic and therapeutic modalities, since they come in very small sizes with modifiable surface, are highly safe and stable, and can be synthesized in a controlled fashion. To date, different nanoparticles have been incorporated into numerous modalities such as tumor‐targeted therapy, thermal therapy, chemotherapy, and radiotherapy. This review article seeks to deliver a brief account of recent advances in research and application of nanoparticles in hyperthermia‐based cancer therapies. The most recent investigations are summarized to highlight the latest advances in the development of combined thermo‐chemo‐radiotherapy, along with the challenges associated with the application of nanoparticles in cancer therapy.This article is categorized under:
Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease
Monitoring Hyperproperties with Prefix Transducers
Authors: Chalupa Marek , Henzinger Thomas A. 
Published: October 2023

AbstractHyperproperties are properties that relate multiple execution traces. Previous work on monitoring hyperproperties focused on synchronous hyperproperties, usually specified in HyperLTL. When monitoring synchronous hyperproperties, all traces are assumed to proceed at the same speed. We introduce (multi-trace) prefix transducers and show how to use them for monitoring synchronous as well as, for the first time, asynchronous hyperproperties. Prefix transducers map multiple input traces into one or more output traces by incrementally matching prefixes of the input traces against expressions similar to regular expressions. The prefixes of different traces which are consumed by a single matching step of the monitor may have different lengths. The deterministic and executable nature of prefix transducers makes them more suitable as an intermediate formalism for runtime verification than logical specifications, which tend to be highly non-deterministic, especially in the case of asynchronous hyperproperties. We report on a set of experiments about monitoring asynchronous version of observational determinism.
Loss of Upk1a and Upk1b expression is linked to stage progression in urothelial carcinoma of the bladder
Authors: Kaczmarek Krystian , Plage Henning , Furlano Kira , Hofbauer Sebastian , Weinberger Sarah , Ralla Bernhard , Franz Antonia , Fendler Annika , de Martino Michela , Roßner Florian , Schallenberg Simon , Elezkurtaj Sefer , Kluth Martina , Lennartz Maximilian , Blessin Niclas C. , Marx Andreas H. , Samtleben Henrik , Fisch Margit , Rink Michael , Slojewski Marcin , Ecke Thorsten , Hallmann Steffen , Koch Stefan , Adamini Nico , Minner Sarah , Simon Ronald , Sauter Guido , Weischenfeldt Joachim , Klatte Tobias , Schlomm Thorsten , Horst David , Zecha Henrik 
Published: October 2023

Abstract
Background
Uroplakin-1a (Upk1a) and uroplakin-1b (Upk1b) have recently been identified as diagnostic markers for the distinction of urothelial carcinomas from other solid tumor entities. Both proteins play an important role in the stabilization and strengthening of epithelial cells that line the bladder.

Methods
To evaluate the prognostic role of uroplakin expression in urothelial carcinomas, more than 2700 urothelial neoplasms were analyzed in a tissue microarray format by immunohistochemistry. To further assess the diagnostic role of uroplakin immunohistochemistry, results were compared with preexisting GATA3 data.

Result
The fraction of Upk1a/Upk1b positive cases decreased slightly from pTaG2 low-grade (88% positive for Upk1a/87% positive for Upk1b) and pTaG2 high-grade (92%/89%) to pTaG3 (83%/88%; p > 0.05) and was lower in muscle-invasive (pT2-4) carcinomas (42%/64%; p < 0.0001/p < 0.0001 for pTa vs. pT2-4). Within pT2-4 carcinomas, high expression of Upk1a and Upk1b was linked to nodal metastasis and lymphatic vessel infiltration (p < 0.05) but unrelated to patient outcome. There were significant associations between Upk1a, Upk1b and GATA3 immunostaining (p < 0.0001 each), but 11% of GATA3 negative cancers were Upk1a/b positive and 8% of Upk1a/b negative cancers were GATA3 positive. Absence of GATA3/Upk1a/b staining was significantly linked to poor patient survival in the subgroup of 126 pT4 carcinomas (p = 0.0004) but not in pT2 and pT3 cancers.

Conclusions
In summary, the results of our study demonstrate that Upk1a and/or Upk1b immunohistochemistry can complement GATA3 for the distinction of urothelial carcinomas. Furthermore, a progressive loss of Upk1a/b expression during stage progression and a prognostic role of the combination GATA3/Upk1a/Upk1b in pT4 carcinomas is evident.
Hydrodynamic regime and cold plasmas hit by short laser pulses
Authors: Fiore Gaetano , De Angelis Monica , Fedele Renato , Guerriero Gabriele , Jovanović Dušan 
Published: October 2023

AbstractWe briefly report and elaborate on some conditions allowing a hydrodynamic description of the impact of a very short and arbitrarily intense laser pulse onto a cold plasma, as well as the localization of the first wave-breaking due to the plasma inhomogeneity. We use a recently developed fully relativistic plane model whereby we reduce the system of the Lorentz–Maxwell and continuity PDEs into a 1-parameter family of decoupled systems of non-autonomous Hamilton equations in dimension 1, with the light-like coordinate $$\xi =ct\!-\!z$$

ξ
=
c
t

-

z

replacing time t as an independent variable. Apriori estimates on the Jacobian $$\hat{J}$$

J
^

of the change from Lagrangian to Eulerian coordinates in terms of the input data (initial density and pulse profile) are obtained applying Liapunov direct method to an associated family of pairs of ODEs; wave-breaking is pinpointed by the inequality $$\hat{J}\le 0$$

J
^

≤
0

. These results may help in drastically simplifying the study of extreme acceleration mechanisms of electrons, which have very important applications.
In-situ gas contents of a multi-section coal seam in Sydney basin for coal and gas outburst management
Authors: Li Zhongbei , Ren Ting , Black Dennis , Qiao Ming , Abedin Itmam , Juric Jessica , Wang Mike 
Published: October 2023

AbstractThe gas content is crucial for evaluating coal and gas outburst potential in underground coal mining. This study focuses on investigating the in-situ coal seam gas content and gas sorption capacity in a representative coal seam with multiple sections (A1, A2, and A3) in the Sydney basin, where the CO2 composition exceeds 90%. The fast direct desorption method and associated devices were described in detail and employed to measure the in-situ gas components (Q1, Q2, and Q3) of the coal seam. The results show that in-situ total gas content (QT) ranges from 9.48 m3/t for the A2 section to 14.80 m3/t for the A3 section, surpassing the Level 2 outburst threshold limit value, thereby necessitating gas drainage measures. Among the gas components, Q2 demonstrates the highest contribution to QT, ranging between 55% and 70%. Furthermore, high-pressure isothermal gas sorption experiments were conducted on coal samples from each seam section to explore their gas sorption capacity. The Langmuir model accurately characterizes CO2 sorption behavior, with fit coefficients (R2) greater than 0.99. Strong positive correlations are observed between in-situ gas content and Langmuir volume, as well as between residual gas content (Q3) and sorption hysteresis. Notably, the A3 seam section is proved to have a higher outburst propensity due to its higher Q1 and Q2 gas contents, lower sorption hysteresis, and reduced coal toughness f value. The insights derived from the study can contribute to the development of effective gas management strategies and enhance the safety and efficiency of coal mining operations.
Online coal dust suppression system for opencast coal mines
Authors: Panicker Senju , Shankar S. S. , Jithin S. , Sandeep S. , Irshad Muhammed , Daniel Jerry , Sajjad Tarique 
Published: October 2023

AbstractCoal is the major source of power in India and world over. Coal mining is an essential industry which has a major role in the economic development of the country. Most major mining activities contribute directly or indirectly to air pollution. Coal dust is a major air pollutant which affects the personal working in the mines and also people residing in villages near the mines. Air pollution due to coal particulates can affect human health and cause damages to the environment. Hence effective pollution control mechanisms are needed to keep the pollution levels within permissible levels. The easiest and most common method employed for dust suppression worldwide is sprinkling of water. In majority of mines, water sprinklers are operated manually and can lead to wastage of water due to over sprinkling. It can also prove to be ineffective in dust suppression if sprinkling is not done properly. The paper proposes a system which can be deployed to automate the dust suppressions sprinklers. The system will monitor the concentration of PM10 and PM2.5 in the air and initiate sprinkling operation when the particulate matter content exceeds preconfigured limits.
Modeling and petrophysical properties of digital rock models with various pore structure types: An improved workflow
Authors: Li Xiaobin , Wei Wei , Xia Yuxuan , Wang Lei , Cai Jianchao 
Published: October 2023

AbstractPore structure is a crucial factor affecting the physical properties of porous materials, and understanding the mechanisms and laws of these effects is of great significance in the fields of geosciences and petroleum engineering. However, it remains a challenge to accurately understand and quantify the relationship between pore structures and effective properties. This paper improves a workflow to focus on investigating the effect of pore structure on physical properties. First, a hybrid modeling approach combining process-based and morphology-based methods is proposed to reconstruct 3D models with diverse pore structure types. Then, the characteristics and differences in pore structure in these models are compared. Finally, the variation laws and pore-scale mechanisms of the influence of pore structure on physical properties (permeability and elasticity) are discussed based on the reconstructed models. The relationship models between pore structure parameters and permeability/elastic parameters in the grain packing model are established. The effect of pore structure evolution on permeability/elasticity and the microscopic mechanism in three types of morphology-based reconstruction models are explored. The influence degree of pore structure on elastic parameters (bulk modulus, shear modulus, P-wave velocity, and S-wave velocity) is quantified, reaching 29.54%, 51.40%, 18.94%, and 23.18%, respectively. This work forms a workflow for exploring the relationship between pore structures and petrophysical properties at the microscopic scale, providing more ideas and references for understanding the complex physical properties in porous media.
A developed dual-site Langmuir model to represent the high-pressure methane adsorption and thermodynamic parameters in shale
Authors: Hu Ke , Zhang Qian , Liu Yufei , Thaika Muhammad Abdurrahman 
Published: October 2023

AbstractComprehending the mechanism of methane adsorption in shales is a crucial step towards optimizing the development of deep-buried shale gas. This is because the methane adsorbed in shale represents a significant proportion of the subsurface shale gas resource. To properly characterize the methane adsorption on shale, which exhibits diverse mineral compositions and multi-scale pore sizes, it is crucial to capture the energy heterogeneity of the adsorption sites. In this paper, a dual-site Langmuir model is proposed, which accounts for the temperature and pressure dependence of the density of the adsorbed phase. The model is applied to the isothermals of methane adsorption on shale, at pressures of up to 30 MPa and temperatures ranging from 40 to 100 °C. The results show that the proposed model can describe the adsorption behavior of methane on shale more accurately than conventional models, which assume a constant value for the density of adsorbed phase. Furthermore, the proposed model can be extrapolated to higher temperatures and pressures. Thermodynamic parameters were analyzed using correctly derived equations. The results indicate that the widely used, but incorrect, equation would underestimate the isosteric heat of adsorption. Neglecting the real gas behavior, volume of the adsorbed phase, and energy heterogeneity of the adsorption sites can lead to overestimation of the isosteric heat of adsorption. Furthermore, the isosteric heat evaluated from excess adsorption data can only be used to make a rough estimate of the real isosteric heat at very low pressure.
Anterior cerebral falx plane in MR images to estimate the craniofacial midline
Authors: Pei Jun , Liao Xu , Ge Lingling , Liu Jianwei , Jiang Xiling 
Published: October 2023

AbstractMultiple methods have been proposed for evaluating the symmetry of facial contour by utilizing the median sagittal plane of the skull as a reference and measuring the maxillofacial region. To replace the manual mark point analysis method, we used the anterior cerebral falx plane in MRI images as an indicator of the craniofacial midline. The MRI examination data of 30 individuals were analyzed with a MeVisLab workstation. Two independent examiners performed 15 anthropometric measurements (4 angular, 11 linear) and compared the MRI-based anterior cerebral falx plane with the manual mark point analysis of the craniofacial midline estimation. All measurements were repeated after 3 weeks. Statistical analyses included the repeatability and reproducibility of the 2 methods based on intra-observer and inter-observer correlation coefficients (ICCs), respectively. Precision was estimated by intergroup comparison of the coefficient of variation. The anterior falx plane derived from the MRI data resulted in an intra-observer ICC of 0.869 ± 0.065 (range 0.733–0.936) and inter-observer ICC of 0.876 ± 0.0417 (0.798–0.932) for all measurements, showing significant correlations with the ICC values obtained by the mark point method (p < 0.05). The coefficient of variation showed that the precisions of the 2 methods were statistically comparable. We conclude that, for MRI-based craniofacial midline estimation, measurements made using the anterior cerebral falx plane are as precise, repeatable, and reproducible as those using the manual mark point analysis method. It has a high potential for application in radiation-free 3-dimensional craniofacial analysis.
RETRACTED ARTICLE: Efficacy and safety of arthroscopy in femoroacetabular impingement syndrome: a systematic review and meta-analysis of randomized clinical trials
Authors: Lamo-Espinosa José María , Mariscal Gonzalo , Gómez-Álvarez Jorge , San-Julián Mikel 
Published: October 2023

AbstractThis study aimed to compare the efficacy and safety of arthroscopy with physiotherapy or joint lavage in patients with femoroacetabular impingement (FAI). A meta-analysis using PubMed, Embase, Scopus, and the Cochrane Collaboration Library databases was performed in September 2022. We included studies focusing on patients with FAI who underwent arthroscopic surgery versus those who underwent physiotherapy or arthroscopic lavage. The outcomes were functional scores (iHOT-33 and HOS ADL) and adverse events. Randomized clinical trials were included in the study. The risk of bias in each study was assessed according to Cochrane guidelines for clinical trials. The data were combined using Review Manager version 5.4. (PROSPERO CRD42022375273). Six RCTs were included, from a pool of 839 patients (407 females). The iHOT-33 and HOS ADL scales showed significant differences at 12 months in favor of the arthroscopy group (MD, 10.65; 95% CI 6.54–4.76) and (MD, 8.09; 95% CI 3.11–13.07). MCID was not achieved through arthroscopy in functional variables. The rates of osteoarthritis (OR, 6.18; 95% CI 1.06–36.00) and numbness (OR, 73.73; 95% CI 10.00–43.92) were significantly higher in the arthroscopy group. Arthroscopic surgery showed statistical superiority over the control group without exceeding the MCID in most studies; however, the results might have been influenced by secondary variables. Finally, arthroscopic surgery results in a high rate of conversion to osteoarthritis.
The significance of heterophasic ion exchange in active biomonitoring of heavy metal pollution of surface waters
Authors: Kłos Andrzej , Wierzba Sławomir , Świsłowski Paweł , Cygan Agnieszka , Gruss Łukasz , Wiatkowski Mirosław , Pulikowski Krzysztof , Ziembik Zbigniew , Dołhańczuk-Śródka Agnieszka , Rajfur Małgorzata , Jerz Dominik , Piechaczek-Wereszczyńska Magdalena , Rosik-Dulewska Czesława , Wieczorek Piotr 
Published: October 2023

AbstractWe have carried out studies to examine the possibility of using biosorbents: the epigeic mosses Pleurozium schreberi (Willd. ex Brid.) Mitt., and the epiphytic lichens Hypogymnia physodes (L.) Nyl. in active biomonitoring of heavy metal pollution of surface waters. The dried sea algae Palmaria palmata (L.) Weber & Mohr were used as the third biosorbent. The studies were conducted in the waters of the Turawa Reservoir, a dam reservoir with a significant level of eutrophication in south-western Poland. Incremental concentrations of Mn, Ni, Zn, Cu, Cd, and Pb were determined in the exposed samples. It was shown that a 2-h exposure period increases the concentration of some metals in the exposed samples, even by as much as several hundred percent. High increments of nickel concentrations in the algae Palmaria palmata (mean: 0.0040 mg/g, with the initial concentration of c0 < 0.0016 in the algae) were noted, with negligible increments in concentrations of this metal in mosses and lichens. In contrast, mosses and lichens accumulated relatively high amounts of Cd (mean: 0.0033 mg/g, c0 = 0.00043 mg/g) and Pb (mean: 0.0243 mg/g, c0 = 0.0103 mg/g), respectively.
Whole transcriptome analysis in offspring whose fathers were exposed to a developmental insult: a novel avian model
Authors: Rimawi Issam , Yanai Sunny , Turgeman Gadi , Yanai Joseph 
Published: October 2023

AbstractAlthough the effects of paternal exposure to insults on the offspring received limited attention in the past, it is currently gaining interest especially after understanding the mechanisms which may mediate such exposure effects. In the current study, the well-controlled avian model (Fayoumi) was utilized to investigate the effects of paternal exposure to the developmental insult, chlorpyrifos on the offspring’s gene expression via mRNA and small RNA sequencing. Numerous mRNA gene expression changes were detected in the offspring after paternal exposure to the developmental insult, especially in genes related to neurogenesis, learning and memory. qPCR analysis of several genes, that were significantly changed in mRNA sequencing, confirmed the results obtained in mRNA sequencing. On the other hand, small RNA sequencing did not identify significant microRNA genes expression changes in the offspring after paternal exposure to the developmental insult. The effects of the paternal exposure were more pronounced in the female offspring compared to the male offspring. The results identified expression alterations in major genes (some of which were pertinent to the functional changes observed in other forms of early developmental exposure) after paternal insult exposure and provided a direction for future studies involving the most affected genes.
Influence of variant-specific mutations, temperature and pH on conformations of a large set of SARS-CoV-2 spike trimer vaccine antigen candidates
Authors: Stuible Matthew , Schrag Joseph D. , Sheff Joey , Zoubchenok Daria , Lord-Dufour Simon , Cass Brian , L’Abbé Denis , Pelletier Alex , Rossotti Martin A. , Tanha Jamshid , Gervais Christian , Maurice Roger , El Bakkouri Majida , Acchione Mauro , Durocher Yves 
Published: October 2023

AbstractSARS-CoV-2 subunit vaccines continue to be the focus of intense clinical development worldwide. Protein antigens in these vaccines most commonly consist of the spike ectodomain fused to a heterologous trimerization sequence, designed to mimic the compact, prefusion conformation of the spike on the virus surface. Since 2020, we have produced dozens of such constructs in CHO cells, consisting of spike variants with different mutations fused to different trimerization sequences. This set of constructs displayed notable conformational heterogeneity, with two distinct trimer species consistently detected by analytical size exclusion chromatography. A recent report showed that spike ectodomain fusion constructs can adopt an alternative trimer conformation consisting of loosely associated ectodomain protomers. Here, we applied multiple biophysical and immunological techniques to demonstrate that this alternative conformation is formed to a significant extent by several SARS-CoV-2 variant spike proteins. We have also examined the influence of temperature and pH, which can induce inter-conversion of the two forms. The substantial structural differences between these trimer types may impact their performance as vaccine antigens.