bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined Effect of Biochar and Plant Growth-Promoting Rhizbacteria on Physiological Responses of Canola (Brassica napus L.) Subjected to Drought Stress
Authors: Gul-Lalay  , Ullah Sami , Shah Sikandar , Jamal Aftab , Saeed Muhammad Farhan , Mihoub Adil , Zia Adil , Ahmed Iftikhar , Seleiman Mahmoud F. , Mancinelli Roberto , Radicetti Emanuele 
Published: January 2024

AbstractBiochar (BC) and plant growth-promoting microbes (PGPR) could represent a suitable agronomical strategy to mitigate the impacts of drought in arid agro-environmental conditions. However, there is currently little understanding of the synergistic benefit of combining BC and PGPR to increase drought tolerance in oilseeds. In this study, the physiological response of two water-stressed canola (Brassica napus L.) plants subjected to the application of BC obtained from waste wood of Morus alba applied solely or in combination with PGPR strains (Pseudomonas sp.) was evaluated. The experiment consists of two genotypes and nine treatments [(C-Control, T1-15 days drought (15DD), T2-30 days drought (30DD), T3-15 days of drought + PG (15DD + PG), T4-30 days of drought + PG (30DD + PG), T5-15 days drought + biochar (15DD + BC), T6-30 days drought + biochar (30DD + BC), T7-15 days drought + biochar + PG (15DD + BC + PG), T8-30 days drought + biochar + PG (30DD + BC + PG)]. Drought stress decreased emergence energy (EE), leaf area index (LAI), leaf area ratio (LAR), root shoot ratio (RSR), moisture content of leaves (MCL), percent moisture content (%MC), moisture content of shoot (MCS) and moisture content of root (MCR), and relative water content (RWC) in both varieties of Brassica napus L., which in contrast, it is increased by the collective application of both biochar and PGPR. In both varieties, N, P, K, Mg, and Ca concentrations were highest in all the biochar and PGPRs separate and combined treatments, while lowest in 15 and 30 days drought treatments. Osmolyte contents like Glycine betaine (GB) and sugar remarkably increased in the stress condition and then reduced due to the synergistic application of biochar and PGPR. Drought stress has a repressive effect on the antioxidant enzymatic system like Peroxidase (POD), Superoxide dismutase (SOD), and glutathione reductase (GR) as well as total flavonoids, phenolics, and protein content. The antioxidant enzymes and phenolic compounds were dramatically increased by the combined action of biochar and PGPRs. A significant increase in EE, LAR, RSR, and RWC under 15 and 30 days drought conditions, evidently highlighting the synergistic effect of BC and PGPR. The results conclude a substantial and positive effect of the combined use of BC and PGPR strains on canola's response to induced drought stress, by regulating the physiological, biochemical, and agronomic traits of the plants.
Graphical Abstract
Hormonal Regulation of Ovule Initiation in Arabidopsis
Authors: Barro-Trastoy Daniela , Gomez Maria Dolores , Tornero Pablo , Perez-Amador Miguel A. 
Published: January 2024

AbstractAs seed precursors, ovules are fundamental organs during the plant life cycle. Decades of morphological and molecular study have allowed for the elucidation of the complex and intricate genetic network regulating ovule development. Ovule and seed number is highly dependent on the number of ovule primordia that are determined from the placenta during early pistil development. Ovule initiation is positively regulated by the plant hormones auxins, cytokinins, and brassinosteroids, as well as negatively regulated by gibberellins. Each hormone does not act independently; multiple points of hormonal crosstalk occur to coordinately regulate ovule primordia initiation. In this review, we highlight the roles of these hormones and their interactions in the genetic and hormonal network co-regulating ovule initiation in Arabidopsis.
The Oriented Diameter of Graphs with Given Connected Domination Number and Distance Domination Number
Authors: Dankelmann Peter , Morgan Jane , Rivett-Carnac Emily 
Published: January 2024

AbstractLet G be a bridgeless graph. An orientation of G is a digraph obtained from G by assigning a direction to each edge. The oriented diameter of G is the minimum diameter among all strong orientations of G. The connected domination number $$\gamma _c(G)$$

γ
c

(
G
)

of G is the minimum cardinality of a set S of vertices of G such that every vertex of G is in S or adjacent to some vertex of S, and which induces a connected subgraph in G. We prove that the oriented diameter of a bridgeless graph G is at most $$2 \gamma _c(G) +3$$

2

γ
c

(
G
)

+
3

if $$\gamma _c(G)$$

γ
c

(
G
)

is even and $$2 \gamma _c(G) +2$$

2

γ
c

(
G
)

+
2

if $$\gamma _c(G)$$

γ
c

(
G
)

is odd. This bound is sharp. For $$d \in {\mathbb {N}}$$

d
∈
N

, the d-distance domination number $$\gamma ^d(G)$$

γ
d

(
G
)

of G is the minimum cardinality of a set S of vertices of G such that every vertex of G is at distance at most d from some vertex of S. As an application of a generalisation of the above result on the connected domination number, we prove an upper bound on the oriented diameter of the form $$(2d+1)(d+1)\gamma ^d(G)+ O(d)$$

(
2
d
+
1
)

(
d
+
1
)

γ
d

(
G
)

+
O

(
d
)

. Furthermore, we construct bridgeless graphs whose oriented diameter is at least $$(d+1)^2 \gamma ^d(G) +O(d)$$

(
d
+
1
)

2

γ
d

(
G
)

+
O

(
d
)

, thus demonstrating that our above bound is best possible apart from a factor of about 2.
Drought projections for the NW Iberian Peninsula under climate change
Authors: Alvarez I. , Pereira H. , Lorenzo M. N. , Picado A. , Sousa M. C. , Taboada J. J. , Dias J. M. 
Published: January 2024

AbstractDrought can be considered an atmospheric condition, which rapidly goes beyond to affect multiple fields of the environment and human activities. The persistence of these atmospheric conditions can affect the recharge of surface and groundwater bodies due to a decrease in the volume of runoff and seepage, thus affecting human and environmental activities. In this context, the main aim of this work is to characterize the occurrence and variability of future droughts in Galicia over the twenty-first century. The methodology followed is based on the calculation and analysis of the Standardized Precipitation Index (SPI) in three-time scales (3, 6, and 12 months), using daily outputs of precipitation data from one RCM provided by the EURO-CORDEX project for different periods (reference from 1971 to 2005, and future from 2025 to 2060 and 2061 to 2096) and scenarios (RCP 4.5 and RCP 8.5). Using SPI-3, SPI-6, and SPI-12 projections, drought trends and potential changes in their characteristics were explored under RCP 4.5 and RCP 8.5 scenarios. In both scenarios, throughout the twenty-first century, a decreasing trend in SPI indicates an intensification of drought conditions over Galicia. Moreover, at the beginning of the century, under RCP 4.5, drought events will increase and will be slightly more intense but less persistent, while under RCP 8.5, the number of events will be almost the same, but shorter and less severe. Toward the end of the century, drought events are expected to be more numerous, less durable, and more intense under both scenarios.
Nomogram for predicting the overall survival and cancer-specific survival of patients with intraductal carcinoma of the prostate
Authors: Cui Yongqiang , Lin Junyang , Sun Dingqi , Zhang Hui , Diao Tongxiang , Fu Qiang 
Published: January 2024

Abstract
Purpose
Intraductal carcinoma of the prostate (IDC-P) is a histological subtype that differs from conventional acinar adenocarcinoma in terms of its origin, appearance, and pathological features. For IDC-P, there is currently no recognized best course of action, and its prognosis is unclear. The goal of this study is to analyze independent prognostic factors in IDC-P patients and to develop and validate a nomogram to predict overall survival (OS) and cancer-specific survival (CSS).

Methods
Clinical data for IDC-P patients were collected from the Surveillance, Epidemiology, and End Results database. To identify the independent variables influencing prognosis, multivariate Cox regression analysis was performed. A nomogram model was created utilizing these variables after comparing the variations in OS and CSS among various subgroups using Kaplan‒Meier curves. Internal validation of the nomograms was verified using the bootstrap resampling method.

Results
The study included 280 IDC-P patients in total. Marital status, summary stage, grade, and the presence of lung metastases were significant factors impacting OS, and CSS was significantly influenced by marital status, summary stage, AJCC stage, the presence of lung metastases, the presence of bone metastases, and PSA according to univariate and multivariate Cox regression models (P < 0.05). Nomogram models were created to estimate OS and CSS using these parameters. The OS prediction model’s C-index was 0.744, whereas the CSS prediction model’s C-index was 0.831.

Conclusion
We developed and verified nomogram models for the prediction of 1-, 3-, and 5-year OS and CSS in patients with IDC-P. These nomograms serve as a resource for evaluating patient prognosis, therapy, and diagnosis, ultimately improving clinical decision-making accuracy.
Concordance of assessments of four PD-L1 immunohistochemical assays in esophageal squamous cell carcinoma (ESCC)
Authors: Wang Xinran , He Jiankun , Li Jinze , Wu Chun , Yue Meng , Niu Shuyao , Jia Ying , Jia Zhanli , Cai Lijing , Liu Yueping 
Published: January 2024

Abstract
Objective
Given real-world limitations in programmed death-ligand 1 (PD-L1) testing, concordance studies between PD-L1 assays are needed. We undertook comparisons of PD-L1 assays (DAKO22C3, Ventana SP263, Ventana SP142, E1L3N) among observers in esophageal squamous cell carcinoma (ESCC) to provide information on the analytical and clinical comparability of four PD-L1 IHC assays.

Methods
Paraffin embedded samples of 50 cases of esophageal squamous cell carcinoma were obtained, satined with all four PD-L1 assays. PD-L1 was evaluated by 68 pathologists from 19 different hospitals. PD-L1 expression was assessed for combined positive score (CPS).

Results
The expression sensitivity of SP263 was the highest in ESCC, followed by 22C3, E1L3N and SP142. Taking CPS 10 as the critical value, inter-observer concordance for CPS scores among 68 physicians was assessed for the 22C3, SP263, SP142, and E1L3N assays, yielding values of 0.777, 0.790, 0.758, and 0.782, respectively. In the comparison between assays, the overall CPS scores concordance rates between 22C3 and SP263, SP142, and E1L3N were 0.896, 0.833, and 0.853, respectively. 22C3 and SP263 have high concordance, with OPA of 0.896, while E1L3N and SP142 have the highest concordance, with OPA of 0.908.

Conclusion
In ESCC, the concordance of PD-L1 evaluation among observers is good, and the immune cell score is still an important factor affecting the concordance of interpretation among observers. Cases near the specific threshold are still the difficult problem of interpretation. SP263 had the highest CPS score of the four assays. SP263 cannot identify all 22C3 positive cases, but had good concordance with 22C3.E1L3N and SP142 showed high concordance.
A parsimonious, computationally efficient machine learning method for spatial regression
Authors: Žukovič Milan , Hristopulos Dionissios T. 
Published: January 2024

AbstractWe introduce the modified planar rotator method (MPRS), a physically inspired machine learning method for spatial/temporal regression. MPRS is a non-parametric model which incorporates spatial or temporal correlations via short-range, distance-dependent “interactions” without assuming a specific form for the underlying probability distribution. Predictions are obtained by means of a fully autonomous learning algorithm which employs equilibrium conditional Monte Carlo simulations. MPRS is able to handle scattered data and arbitrary spatial dimensions. We report tests on various synthetic and real-word data in one, two and three dimensions which demonstrate that the MPRS prediction performance (without hyperparameter tuning) is competitive with standard interpolation methods such as ordinary kriging and inverse distance weighting. MPRS is a particularly effective gap-filling method for rough and non-Gaussian data (e.g., daily precipitation time series). MPRS shows superior computational efficiency and scalability for large samples. Massive datasets involving millions of nodes can be processed in a few seconds on a standard personal computer. We also present evidence that MPRS, by avoiding the Gaussian assumption, provides more reliable prediction intervals than kriging for highly skewed distributions.
Degenerate stability of the Caffarelli–Kohn–Nirenberg inequality along the Felli–Schneider curve
Authors: Frank Rupert L. , Peteranderl Jonas W. 
Published: January 2024

AbstractWe show that the Caffarelli–Kohn–Nirenberg (CKN) inequality holds with a remainder term that is quartic in the distance to the set of optimizers for the full parameter range of the Felli–Schneider (FS) curve. The fourth power is best possible. This is due to the presence of non-trivial zero modes of the Hessian of the deficit functional along the FS-curve. Following an iterated Bianchi–Egnell strategy, the heart of our proof is verifying a ‘secondary non-degeneracy condition’. Our result completes the stability analysis for the CKN-inequality to leading order started by Wei and Wu. Moreover, it is the first instance of degenerate stability for non-constant optimizers and for a non-compact domain.
Disposition effect and its outcome on endogenous price fluctuations
Authors: Cafferata Alessia , Patacca Marco , Tramontana Fabio 
Published: January 2024

AbstractWe have developed a financial market model that incorporates the Disposition Effect, which refers to traders’ tendency to avoid realizing losses. Specifically, our model replicates several stylized facts commonly observed in financial markets, such as fat tails and volatility clustering. These market characteristics can be attributed to the Disposition Effect, especially when the trading behavior of agents aligns with the findings of Ben-David and Hirshleifer (Rev Financ Stud 25(8):2485–2532, 2012). To demonstrate this, we examine two versions of the model: one where a class of agents exhibits a high degree of Disposition Effect and another where traders are not influenced by it. By comparing the simulated time series generated by both versions, we find that the one with agents affected by the Disposition Effect better replicates the features observed in real financial markets. This holds true for both the deterministic and stochastic versions of the model.
Ascites fluid calprotectin level is highly accurate in diagnosing spontaneous bacterial peritonitis: a preliminary proof of concept prospective study
Authors: Sbeit Wisam , Maamoun Basheer , Azzam Subhi , Shahin Amir , Carmiel-Haggai Michal , Khoury Tawfik 
Published: January 2024

AbstractAscites is the most common complication of liver cirrhosis. Spontaneous bacterial peritonitis (SBP) is a common complication of ascites. The diagnosis is made by an ascitic fluid polymorphonuclear (PMN) cell count of ≥ 250/mm3. However, no other diagnostic test is present for the diagnosis of SBP. The aim of the study present study is to assess the diagnostic yield of ascitic calprotectin in SBP, and to explore whether it can predict disease stage. We performed a single center proof-of-concept prospective study including all patients with cirrhosis and ascites who underwent paracentesis. Overall, 31 patients were included in the study. Eight patients had SBP vs. 23 patients without SBP. Ascitic calprotectin level was 77.4 ± 86.5 μg/mL in the SBP group, as compared to 16.1 ± 5.6 μg/mL in the non-SBP group (P = 0.001). An ascitic calprotectin cut-off value of > 21 μg/mL was associated with sensitivity and specificity of 85.7% and 89.5%, respectively, with ROC of 0.947 (95% CI 0.783 to 0.997, P < 0.0001). Notably, ascitic calprotectin did not had a prognostic value in cirrhosis stage and prognosis. Ascitic calprotectin was highly accurate in the diagnosis of SBP. It can be a serve as adjunct for indefinite cases of SBP.
Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
Authors: Lin Hugo Y.-H. , Liang Chan-Jung , Yang Ming-Yu , Chen Phang-Lang , Wang Tzu-Ming , Chen Yen-Hua , Shih Yao-Hsiang , Liu Wangta , Chiu Chien-Chih , Chiang Chih-Kang , Lin Chang-Shen , Lin Han-Chen 
Published: January 2024

AbstractMaleic acid (MA) induces renal tubular cell dysfunction directed to acute kidney injury (AKI). AKI is an increasing global health burden due to its association with mortality and morbidity. However, targeted therapy for AKI is lacking. Previously, we determined mitochondrial-associated proteins are MA-induced AKI affinity proteins. We hypothesized that mitochondrial dysfunction in tubular epithelial cells plays a critical role in AKI. In vivo and in vitro systems have been used to test this hypothesis. For the in vivo model, C57BL/6 mice were intraperitoneally injected with 400 mg/kg body weight MA. For the in vitro model, HK-2 human proximal tubular epithelial cells were treated with 2 mM or 5 mM MA for 24 h. AKI can be induced by administration of MA. In the mice injected with MA, the levels of blood urea nitrogen (BUN) and creatinine in the sera were significantly increased (p < 0.005). From the pathological analysis, MA-induced AKI aggravated renal tubular injuries, increased kidney injury molecule-1 (KIM-1) expression and caused renal tubular cell apoptosis. At the cellular level, mitochondrial dysfunction was found with increasing mitochondrial reactive oxygen species (ROS) (p < 0.001), uncoupled mitochondrial respiration with decreasing electron transfer system activity (p < 0.001), and decreasing ATP production (p < 0.05). Under transmission electron microscope (TEM) examination, the cristae formation of mitochondria was defective in MA-induced AKI. To unveil the potential target in mitochondria, gene expression analysis revealed a significantly lower level of ATPase6 (p < 0.001). Renal mitochondrial protein levels of ATP subunits 5A1 and 5C1 (p < 0.05) were significantly decreased, as confirmed by protein analysis. Our study demonstrated that dysfunction of mitochondria resulting from altered expression of ATP synthase in renal tubular cells is associated with MA-induced AKI. This finding provides a potential novel target to develop new strategies for better prevention and treatment of MA-induced AKI.
What Mental Health and Wellbeing Interventions Work for Which Children and Young People in Care? Systematic Review of Potential Outcome Inequities
Authors: Evans Rhiannon , Trubey Rob , MacDonald Sarah , Noyes Jane , Robling Michael , Willis Simone , Boffey Maria , Wooders Charlotte , Vinnicombe Soo , Melendez-Torres G. J. 
Published: January 2024

AbstractChildren and young people with care-experience (e.g. foster, kinship and residential care) report poorer mental health and wellbeing than the general population. Despite an emerging evidence-base for intervention, it is not clear if current approaches create, exacerbate or mitigate outcome inequities between different types of participants. We conducted a systematic review of international interventions targeting mental health, subjective wellbeing and suicide-related outcomes amongst care-experienced children and young people aged up to 25 years old. The review included a narrative synthesis of intervention inequities, exploring if they were more or less effective for different participant groups. Eight interventions, with 14 study reports, presented relevant data. Overall, there was no clear evidence that intervention participation could lead to inequitable impacts, being more or less effective for different groups. However, there was some tentative indication that individuals with lower exposure to maltreatment, fewer care placements, and increased baseline mental health problems, might be more responsive to intervention than other participants. There was limited evidence for wellbeing and no data availability for suicide. Future intervention evaluation should focus on assessing if there is potential to create, sustain or exacerbate inequities, and how approaches may be designed to mitigate this risk.
Production of derivatives from wheat straw as reinforcement material for paper produced from secondary fibers
Authors: Serrano Iciar , Afailal Zainab , Sánchez-Paniagua Naiara , González Pablo , Bautista Ana , Gil-Lalaguna Noemí , Gonzalo Alberto , Arauzo Jesús , Crespo Cristina , Sánchez Jose Luís 
Published: January 2024

AbstractNowadays, a high percentage (> 50%) of the paper produced in Europe uses recovered paper (secondary fibers) as raw material. In order to improve the mechanical properties of the paper produced, different kinds of additives are usually incorporated into the paper. Emerging renewable materials based on agricultural or forest residues, such as cellulose nanomaterials, have recently proved good capacities as reinforcing agents for different applications. In this work, pulp from wheat straw with a content of cellulose nanomaterial has been produced and tested as a mechanical reinforcing agent for paper production. A soda semi-chemical process was applied for the delignification of straw, to produce pulp with high cellulose content. Posteriorly, pulps with cellulose nanofibers were obtained in a high-pressure homogenizer, applying three different pretreatments to the cellulose pulp (acid hydrolysis, enzymatic hydrolysis and thermal treatment with glycerol) in order to facilitate the obtention of cellulose nanomaterial. Handsheets of paper were prepared from two sources of secondary fiber (fluting paper and old corrugated containers), adding different percentages of wheat straw derivatives (0, 3.5, 5 and 7%). The fibers' morphology and the papers' mechanical properties were investigated. Noticeable improvement rates (up to 25%) were observed for some mechanical properties of paper containing nanocellulose produced after the enzymatic and acid pretreatments. The quality of the secondary fibers source also affected the improvement rates achieved, with higher percentage changes for the lower-quality recycled paper.
Clinical applications of circulating tumor cells in patients with solid tumors
Authors: Smit Daniel J. , Schneegans Svenja , Pantel Klaus 
Published: January 2024

AbstractThe concept of liquid biopsy analysis has been established more than a decade ago. Since the establishment of the term, tremendous advances have been achieved and plenty of methods as well as analytes have been investigated in basic research as well in clinical trials. Liquid biopsy refers to a body fluid-based biopsy that is minimal-invasive, and most importantly, allows dense monitoring of tumor responses by sequential blood sampling. Blood is the most important analyte for liquid biopsy analyses, providing an easily accessible source for a plethora of cells, cell-derived products, free nucleic acids, proteins as well as vesicles. More than 12,000 publications are listed in PubMed as of today including the term liquid biopsy. In this manuscript, we critically review the current implications of liquid biopsy, with special focus on circulating tumor cells, and describe the hurdles that need to be addressed before liquid biopsy can be implemented in clinical standard of care guidelines.