bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma
Authors: Paydas Semra , Ergin Melek , Tanriverdi Kahraman , Yavuz Sinan , Disel Umut , Kilic Nil Banu , Erdogan Seyda , Sahin Berksoy , Tuncer İlhan , Burgut Refik 
Published: June 2004

AbstractThe aim of this study is to detect the possible role of hepatitis C Virus (HCV) in lymphomagenesis. HCV‐RNA and anti‐HCV antibodies were studied in tissue and serum samples taken from patients with non‐Hodgkin's Lymphoma (NHL). The prevalence of HCV, the clinical presentation of these cases, and association with histologic subtypes were determined. RT‐PCR was used to detect the HCV‐RNA in serum and tissue samples. The anti‐HCV antibodies were tested with microparticle enzyme immunoassay. Immunohistochemistry with the ABC method was used to detect the HCV core protein in HCV‐RNA+ cases. RNA could be detected in 30 of 35 cases, and other tests were performed in these 30 samples. HCV‐RNA was detected in 11 tissue samples (11/30, 37%). HCV core protein was studied in 10 of 11 HCV‐RNA+ cases, and 1–3% nuclear staining was found in only 2 samples. Serologically, HCV‐RNA was detected in 7 of 30 samples (23.3%) and anti‐HCV antibody was detected in 3 of 30 samples (10%). Detection of HCV‐RNA in 37% of the lymphoma tissue samples suggests that HCV may have a role or is a contributing factor in the pathogenesis of lymphoma. The very low HCV core protein in lymphoma tissues may be due to the low viral load in lymphoid tissues and/or higher sensitivity of the PCR method. Detection of anti‐HCV antibody in only three cases may be associated with undetectable levels of antibodies due to the immune deficiency in cases with NHL. Am. J. Hematol. 76:252–257, 2004. © 2004 Wiley‐Liss, Inc.
Simplified assay for VWF cleaving protease (ADAMTS13) activity and inhibitor in plasma
Authors: Knovich Mary Ann , Craver Karen , Matulis Melissa D. , Lawson Heather , Owen John 
Published: June 2004

AbstractThe absence of VWF cleaving protease activity (VWFcp, ADAMTS13) has recently been identified as a central component in the pathogenesis of TTP. Several assays for the measurement of ADAMTS13 activity have been described, but most are cumbersome and employ techniques not easily adapted to routine laboratories. Thus, ADAMTS13 assays are available at only a few reference or research laboratories. Prompt identification of absent or impaired ADAMTS13 activity could prove invaluable to clinical decision‐making regarding diagnosis and treatment of suspected TTP patients. We describe an assay for ADAMTS13 that uses a commercial source of VWF substrate and obviates dialysis for sample and substrate preparation. Patient and normal plasma are prepared by desalting over Micro‐Spin columns, and barium is added to activate the cleaving protease. Humate‐P® is prepared over a desalting column and reconstituted in urea prior to use as the exogenous VWF substrate. Desalted plasma is mixed with prepared substrate, and digestion is carried out at 37°C. Maximum sensitivity to very low levels of enzyme activity is achieved by using undiluted plasma and allowing digestion to proceed for 14 hr. The extent of VWF multimer cleavage is then demonstrated by Western blot. We used this assay to analyze VWF cleaving protease activity in plasma samples from 30 patients treated at our institution for TTP. Plasma from patients lacking ADAMTS13 was incubated with PNP and then analyzed by the same methods to determine the presence of an inhibitor. Our results indicate that the assay is suitable for providing timely information regarding ADAMTS13 activity for the diagnosis and treatment of patients with suspected TTP. Am. J. Hematol. 76:286–290, 2004. © 2004 Wiley‐Liss, Inc.
Homoharringtonine mediates myeloid cell apoptosis via upregulation of pro‐apoptotic bax and inducing caspase‐3‐mediated cleavage of poly(ADP‐ribose) polymerase (PARP)
Authors: Yinjun Lou , Jie Jin , Weilai Xu , Xiangming Tong 
Published: June 2004

AbstractHomoharringtonine (HHT) is a plant alkaloid with antileukemia activity that is currently being used for treatment of acute, chronic leukemias and MDS. In this study, we show that HHT can induce apoptosis in a variety of human myeloid leukemia cell lines (U937, HL‐60, HEL, THP, and K562). U937 and HL60 cells undergo rapid apoptosis on treatment with HHT, as indicated by increased annexin V binding capacity, caspase‐3 activation, and cleavage of poly(ADP‐ribose) polymerase (PARP). In addition, the expression of bax is upregulated during HHT‐induced cell death, whereas the expression of bcl‐2 is only slightly decreased. Importantly, treatment of primary leukemic cells, obtained from acute myeloid leukemia patients, resulted in rapid apoptosis. Thus, our data provide the mechanism of HHT and justify the use of HHT in the treatment of human myeloid leukemia. Am. J. Hematol. 76:199–204, 2004. © 2004 Wiley‐Liss, Inc.
Localized relapse in bone marrow of extremities after allogeneic stem cell transplantation for acute lymphoblastic leukemia
Authors: Endo T. , Sato N. , Koizumi K. , Nishio M. , Fujimoto K. , Sakai T. , Kumano K. , Obara M. , Minauchi K. , Koike T. 
Published: June 2004

AbstractWe report a patient with a relapsed in bone marrow of extremities after allogeneic peripheral blood stem cell transplantation for acute lymphoblastic leukemia (ALL). The patient complained of pain in the right upper arm and left leg 15 months after transplantation. Magnetic resonance imaging (MRI) and fluorine‐18 fluorodeoxyglucose positron emission tomography (FDG‐PET) showed abnormal findings in bone marrow of upper and lower extremities. There were no findings of relapse in aspirates from the sternum and iliac bone marrow. Biopsy specimen from the iliac bone marrow showed normocellular marrow without leukemic cells. Biopsy specimen from the right humerus revealed marked leukemic cell infiltration in the bone marrow. This is apparently the first case of localized relapse of ALL in bone marrow of extremities. Physicians should be aware of unusual relapse sites of leukemia after allogeneic stem cell transplantation. MRI and FDG‐PET may be of value in detecting this type of relapse. Am. J. Hematol. 76:279–282, 2004. © 2004 Wiley‐Liss, Inc.
Membranous glomerulonephritis in chronic lymphocytic leukemia
Authors: Ziakas Panayiotis D. , Giannouli Stavroula , Psimenou Erasmia , Nakopoulou Lydia , Voulgarelis Michalis 
Published: June 2004

AbstractWe present a case of chronic lymphocytic leukemia associated with nephrotic syndrome. Renal biopsy revealed membranous glomerulonephritis accompanied by interstitial monoclonal lymphocytic infiltration. Combination therapy with cyclophosphamide, vincristine, and prednisone (COP) was successful in inducing an effective response in chronic lymphocytic leukemia. Improvement of renal function and proteinuria was also observed. Am. J. Hematol. 76:271–274, 2004. © 2004 Wiley‐Liss, Inc.
Pregnancy‐related thrombosis in a woman with congenital afibrinogenemia: A report of two successful pregnancies
Authors: Roqué H. , Stephenson C. , Lee M.J. , Funai E.F. , Popiolek D. , Kim E. , Hart D. 
Published: June 2004

AbstractWe managed two pregnancies in a woman with congenital afibrinogenemia with increasing amounts of cryoprecipitate to achieve a pre‐infusion fibrinogen level of 60 mg/dL. The first pregnancy resulted in placental abruption at 36 weeks, in spite of recent cryoprecipitate infusion. Both placentas showed infarction. Post‐partum ovarian and renal vein thromboses complicated the second pregnancy. Mean FVIII (344%) and vWF Antigen (323%) were elevated prior to cryoprecipitate infusion, with mean post‐infusion levels of 367% and 363%. The clearance of fibrinogen after cryoprecipitate infusion increased during the course of pregnancy. A paradoxical prothrombotic state with embolization may play a role in the observed complications of pregnancy. Am. J. Hematol. 76:267–270, 2004. © 2004 Wiley‐Liss, Inc.
Echocardiographic abnormalities in sickle cell disease
Authors: Ahmed Shahid , Siddiqui Anita K. , Sadiq Adnan , Shahid Rabia K. , Patel Dilip V , Russo Linda A. 
Published: June 2004

AbstractEchocardiographic abnormalities in patients with sickle cell disease (SCD) were determined, and pulmonary arterial systolic pressure (PASP) was estimated. Clinical data and echocardiograms of 38 adult hospitalized patients with SCD at two tertiary care hospitals were reviewed. Fisher's exact test was performed to determine correlation between pulmonary hypertension and various clinical variables. Pulmonary hypertension was the most common abnormality identified in 22 (58%) patients. The estimated mean PASP was 37.5 ± 10.9 mmHg. Older age and prior history of acute chest syndrome were significantly correlated with an increased prevalence of pulmonary hypertension (P < 0.05). Patients with hemoglobin levels <8 g/dL had PASP 43.2 ± 0.5 compared to a mean PASP of 33.3 ± 6.0 in patients with hemoglobin ≥8 g/dL (P = 0.01). Eight (21%) patients had evidence of a hyperdynamic left ventricle. Left heart abnormalities included dilated atrium in 14 (37%), dilated ventricle in 5 (13%), ventricle hypertrophy in 5 (13%), and ventricle dysfunction in 3 (9%) patients. Right heart abnormalities included dilated atrium in 9 (24%), dilated ventricle in 6 (16%), and ventricle dysfunction in 3 (9%) patients. Despite an increased incidence of abnormal flow across the valves on Doppler analysis, no patient had structurally abnormal valves. A majority of patients with SCD had evidence of pulmonary hypertension, which correlated with older age and history of acute chest syndrome. Other structural and functional echocardiographic abnormalities were less common. Am. J. Hematol. 76:195–198, 2004. © 2004 Wiley‐Liss, Inc.
Idiopathic light‐chain proteinuria: Case report and review of the literature
Authors: Audard Vincent , Damaj Ghandi , Rubio Marie Thérèse , Aucouturier Pièrre , Hermine Olivier , Varet Bruno 
Published: June 2004

AbstractIsolated Bence Jones proteinuria (BJP) is a distinct form of monoclonal gammopathy of undetermined significance (MGUS) rarely described. The prognosis of the disease is apparently good. We report here a case of idiopathic BJP and review the literature. Am. J. Hematol. 76:293–294, 2004. © 2004 Wiley‐Liss, Inc.
Adaptive skills, cognitive, and behavioral characteristics of Costello syndrome
Authors: Axelrad Marni E. , Glidden Rochelle , Nicholson Linda , Gripp Karen W. 
Published: June 2004

AbstractCostello syndrome is a rare congenital disorder whose diagnosis is based on clinical findings. The underlying genetic cause has not been identified. Common characteristics include failure to thrive, feeding problems, short stature, coarsening of facial features, developmental delay, mental retardation, skeletal problems, cardiac complications, and increased risk for solid tumors. Given its rarity, existing literature is limited regarding its natural course. Developmental milestones and intellectual functioning have never been systematically studied in this population, therefore this study focused on defining developmental and intellectual attributes. Data was obtained through solicited participation at the 3rd International Costello Syndrome Meeting. A total of 18 children (age 3–20 years) were administered the Leiter‐R brief‐IQ and the Peabody picture vocabulary test (PPVT)‐III to assess nonverbal cognitive functioning and receptive vocabulary. Parents/guardians completed the child behavior checklist (CBCL) and participated in an interview process to complete the survey form of the Vineland adaptive behavior scales to assess emotional/behavioral issues and adaptive behavior. Results indicated that nonverbal cognitive functioning ranged from 1.5 to 4.3 SD below the age mean. Receptive vocabulary skills ranged from average functioning to 4 SD below the age mean. Adaptive behavior composites were all in the low range (2.0 to >5.0 SD below age mean), however there was significant variability in the range of domain scores. CBCL results ranged from normal to clinically significant across various clinical parameters. These data support a generalized descriptive pattern of developmental delay and mental retardation, with noted variability in receptive language, level of adaptive behavior functioning, and emotional/behavioral aspects. Skills and behavior are compared to existing literature of children with various syndromes. © 2004 Wiley‐Liss, Inc.
Growth hormone deficiency in Costello syndrome
Authors: Stein Robert I. , Legault Laurent , Daneman Denis , Weksberg Rosanna , Hamilton Jill 
Published: June 2004

AbstractWe report on three patients with Costello syndrome and isolated growth hormone (GH) deficiency treated with biosynthetic GH. To our knowledge, these are the only patients with Costello syndrome who have been successfully treated for GH deficiency. We review the pathophysiology of Costello syndrome and highlight the recent recommendations of tumor screening and cardiac surveillance in this population, of particular relevance to those receiving GH therapy. © 2004 Wiley‐Liss, Inc.
Behavioral pattern profile: A tool for the description of behavior to be used in the genetics clinic
Authors: Shalev Stavit A. , Hall Judith G. 
Published: June 2004

AbstractCareful descriptions of dysmorphic features have led to the delineation of hundreds of specific syndromes and patterns of congenital anomalies. The defining of unusual behavior had largely been neglected by clinical geneticists until the study of the natural history of microdeletions revealed that each has unique and characteristic behavior(s). In this study, a simple tool to describe behavior is presented that is to be used in the genetics clinic as part of routine evaluation. The form is meant to simplify recording and does not require the formal training needed for psychological assessments. It is hoped that routine recording of behavior among individuals seen in the genetics clinic will lead to better recognition of unusual behavior among individuals with known conditions, as well as the recognition of conditions characterized only by unusual behavior. Better description of behavioral patterns should lead to the ascertainment of homogeneous groups of affected individuals with abnormalities in the functional pathways that generate patterns of reaction. The genetic and biochemical basis for these patterns of reaction (abnormal behavior) should then be available for both natural history and molecular studies. The tool consists of 12 categories of behavioral features that can be assessed by the medical geneticist. The list was built to allow the observation of as many behavioral aspects as possible, but to keep it to a practical size and to use those features that are simple to describe and quantitate. We expect that its use will produce a rich source of behavioral profiles and will eventually contribute to the better understanding of unusual behavior. © 2004 Wiley‐Liss, Inc.
Katalytische Markownikow‐ und Anti‐Markownikow‐Funktionalisierung von Alkenen und Alkinen
Authors: Beller Matthias , Seayad Jayasree , Tillack Annegret , Jiao Haijun 
Published: June 2004

AbstractReaktionen zur selektiven Veredelung von Alkenen zählen zu den wichtigsten Methoden in der Organischen Synthese. Durch die Entwicklung von Übergangsmetallkatalysatoren in den letzten Jahrzehnten können heute alle Arten von Selektivität bei einer Vielzahl von Transformationen effizient gesteuert werden. Auch 100 Jahre nach dem Tod von Vladimir Markownikow – dem Pionier auf diesem Gebiet – ist die regioselektive Funktionalisierung von Alkenen und Alkinen immer noch ein wichtiges Forschungsziel: Besonders die Anti‐Markownikow‐Addition von Sauerstoff‐ und Stickstoff‐Nucleophilen an aliphatische Alkene und Alkine verfügt über großes industrielles Potenzial. In diesem Aufsatz werden neuere Entwicklungen auf diesem Gebiet beschrieben. Eine Auswahl aktueller Beispiele zeigt, wie die Regioselektivität durch die Wahl des Katalysators gesteuert werden kann.
Cartilage canals in the chicken embryo are involved in the process of endochondral bone formation within the epiphyseal growth plate
Authors: Blumer Michael J.F. , Longato Stefano , Fritsch Helga 
Published: June 2004

AbstractA detailed study of so‐called communicating cartilage canals, which penetrate deeply up into the lower hypertrophic zone of the epiphyseal growth plate in the embryonic chicken femur (E20), was carried out with the aim to clarify whether or not these canals are involved in the bone‐forming process. In addition, we examined the manner in which cartilage canals are formed and compare the present data with our previous data. The canals were investigated by means of light microscopy, electron microscopy, immunohistochemistry (VEGF, VEGFR2/Flk1, type I collagen), and 3D reconstruction. Some communicating canals deeply penetrate into the upper hypertrophic zone where they terminate, showing electron‐dense cells at their end. Subcellular characteristics of these cells are hardly detectable and we suppose that they undergo cell death. Other canals pass down deeper into the lower hypertrophic zone. The upper segment of these canals is composed of capillaries, mesenchymal cells, and macrophage‐like cells. Precursors of osteoblasts are adjacent to the canals. The lower segment of communicating canals is composed of bone matrix or osteoid, which contains type I collagen fibrils and cells having the typical subcellular features of osteoblasts. No vessels are found in these segments. Immunohistochemistry shows that the matrix of the canals labels positively for type I collagen. In addition, staining with sirius red demonstrates that bone matrix is formed in these parts. We assume that the osteoblast‐like cells of the lower segments of communicating canals originate either from mesenchymal cells or even from hypertrophic chondrocytes. Our immunohistochemical data also reveal that vascular endothelial growth factor (VEGF) and the corresponding receptor VEGFR2/Flk1 (VEGF receptor 2/Flk1) are localized in cartilage canals of the reserve zone, the proliferative zone, and the hypertrophic zone. The receptor is found in the endothelial cells of the vessels. Furthermore, VEGF is present in hypertrophic chondrocytes. The results of our study suggest that cartilage canals penetrate actively into the cartilage anlage and that bone is formed in the lower segments of the communicating canals where no vessels are detectable. © 2004 Wiley‐Liss, Inc.
Detection of alkanes, alcohols, and aldehydes using bioluminescence
Authors: Minak‐Bernero Vera , Bare Richard E. , Haith Copper E. , Grossman Matthew J. 
Published: June 2004

AbstractWe report a novel method for the rapid, sensitive, and quantitative detection of alkanes, alcohols, and aldehydes that relies on the reaction of bacterial luciferase with an aldehyde, resulting in the emission of light. Primary alcohols with corresponding aldehydes that are within the substrate range of the particular luciferase are detected after conversion to the aldehyde by an alcohol dehydrogenase. In addition, alkanes themselves may be detected by conversion to primary alcohols by an alkane hydroxylase, followed by conversion to the aldehyde by alcohol dehydrogenase. We developed a rapid bioluminescent method by genetically engineering the genes encoding bacterial luciferase, alcohol dehydrogenase, and alkane hydroxylase into a plasmid for simultaneous expression in an E. coli host cell line. Alkanes, alcohols, or aldehydes were detected within seconds, with sensitivity in the micromolar range, by measuring the resulting light emission with a microplate reader. We demonstrate the application of this method for the detection of alkanes, alcohols, and aldehydes and for the detection of alkane hydroxylase and alcohol dehydrogenase activity in vivo. This method is amenable to the high‐throughput screening needs required for the identification of novel catalysts. © 2004 Wiley Periodicals, Inc.
Synergistic activity of Paenibacillus sp. BP‐23 cellobiohydrolase Cel48C in association with the contiguous endoglucanase Cel9B and with endo‐ or exo‐acting glucanases from Thermobifida fusca
Authors: Sánchez Marta M. , Irwin Diana C. , Pastor F.I. Javier , Wilson David B. , Diaz Pilar 
Published: June 2004

AbstractCellobiohydrolase Cel48C from Paenibacillus sp. BP‐23, an enzyme displaying limited activity on most cellulosic substrates, was assayed for activity in the presence of other bacterial endo‐ or exocellulases. Significant enhanced activity was observed when Cel48C was incubated in the presence of Paenibacillus sp. BP‐23 endoglucanase Cel9B or Thermobifida fusca cellulases Cel6A and Cel6B, indicating that Cel48C acts synergistically with them. Maximum synergism rates on bacterial microcrystalline cellulose or filter paper were obtained with a mixture of Paenibacillus cellulases Cel9B and Cel48C, accompanied by T. fusca exocellulase Cel6B. Synergism was also observed in cell extracts from recombinant clone E. coli pUCel9‐Cel48 expressing the two contiguous Paenibacillus cellulases Cel9B and Cel48C. The enhanced cellulolytic activity displayed by the cellulase mixtures assayed could be used as an efficient tool for biotechnological applications like pulp and paper manufacturing. © 2004 Wiley Periodicals, Inc.
Prognostic significance of both surgical and pathological assessment of curative resection for rectal cancer
Authors: Martling A , Singnomklao T , Holm T , Rutqvist L E , Cedermark B 
Published: June 2004

Abstract

Background
Information on whether surgery has been ‘curative’ is essential for prediction of prognosis and for selection of patients for adjuvant treatment. The aim of this study was to analyse the prognostic value of surgeons' and pathologists' assessments of tumour clearance in patients with primary rectal cancer who underwent resection.

Methods
A total of 1550 patients were studied prospectively. Data were collected from reports made by surgeons and pathologists on whether tumour clearance had been ‘complete’, ‘uncertain’ or ‘incomplete’. The predictive value in relation to outcome after at least 5 years of follow-up was assessed.

Results
In patients assessed as having a complete surgical clearance, tumour recurrence developed in 33·3 per cent. For patients with an uncertain or incomplete clearance the recurrence rate was 59·5 and 61 per cent respectively (P &lt; 0·001). The relative risk of recurrence was twice as high when the surgeon and pathologist disagreed than when they both agreed on the complete clearance. Survival in patients with a complete, uncertain or incomplete surgical clearance was 55·3, 23·0 and 10 per cent respectively (P = 0·050).

Conclusion
Assessments of tumour clearance were of strong prognostic value in relation to outcome. When the surgeon or pathologist was uncertain, or there was disagreement about the completeness of clearance, the risk of recurrence was similar to that among patients in whom an incomplete resection had been performed.