bluuebunny/crossref_metadata_2025_split huggingface dataset:
A low‐temperature SPR‐based assay for monoclonal antibody galactosylation and fucosylation assessment using FcγRIIA/B
Authors: Gaudreault Jimmy , Forest‐Nault Catherine , Gilbert Michel , Durocher Yves , Henry Olivier , De Crescenzo Gregory 
Published: February 2024

AbstractMonoclonal antibodies (MAbs) are powerful therapeutic tools in modern medicine and represent a rapidly expanding multibillion USD market. While bioprocesses are generally well understood and optimized for MAbs, online quality control remains challenging. Notably, N‐glycosylation is a critical quality attribute of MAbs as it affects binding to Fcγ receptors (FcγRs), impacting the efficacy and safety of MAbs. Traditional N‐glycosylation characterization methods are ill‐suited for online monitoring of a bioreactor; in contrast, surface plasmon resonance (SPR) represents a promising avenue, as SPR biosensors can record MAb–FcγR interactions in real‐time and without labeling. In this study, we produced five lots of differentially glycosylated Trastuzumab (TZM) and finely characterized their glycosylation profile by HILIC‐UPLC chromatography. We then compared the interaction kinetics of these MAb lots with four FcγRs including FcγRIIA and FcγRIIB at 5°C and 25°C. When interacting with FcγRIIA/B at low temperature, the differentially glycosylated MAb lots exhibited distinct kinetic behaviors, contrary to room‐temperature experiments. Galactosylated TZM (1) and core fucosylated TZM (2) could be discriminated and even quantified using an analytical technique based on the area under the curve of the signal recorded during the dissociation phase of a SPR sensorgram describing the interaction with FcγRIIA (1) or FcγRII2B (2). Because of the rapidity of the proposed method (<5 min per measurement) and the small sample concentration it requires (as low as 30 nM, exact concentration not required), it could be a valuable process analytical technology for MAb glycosylation monitoring.
Assessment of the competitiveness of food products of Ukraine in the domestic and foreign markets
Authors: Musayeva Nazakat , Tanriverdiyeva Gulnara , Faradjova Dilbar , Mammadova Ulkar 
Published: February 2024

AbstractThis article conducts a comprehensive financial analysis of the competitiveness of confectionery producers in Ukraine within the global confectionery market context. It aims to assess the challenges faced by Ukrainian producers and propose practical strategies to enhance their competitiveness. The research employs various methods, including the construction of a competitive market map and the application of the theory of effective competition. Competitiveness coefficients are calculated to evaluate the performance of key players. A SWOT analysis is conducted to identify strengths and weaknesses, and potential measures for increasing competitiveness are proposed. The global confectionery market has reached $298 billion in 2021, but Ukrainian producers have encountered obstacles, such as the COVID‐19 pandemic, rising production costs and geopolitical conflicts, leading to closures and reduced exports. Roshen emerges as the leader with a 24.7% market share in 2022, although all major companies experienced negative growth rates due to the war. Competitiveness coefficients also position Roshen at the forefront, whilst several other plants demonstrate significant potential. The article's practical significance stems from its data‐driven analysis of competitiveness, providing valuable insights and strategies for managers to improve the production and export capabilities of Ukrainian confectionery producers in the face of global market challenges. Future research could explore sustainable competitive advantages and cost reduction strategies.
Recent Understanding of Water‐Assisted CO2 Hydrogenation to Alcohols
Authors: Vieira Luiz H. , da Silva Alisson H. M. , Santana Cássia S. , Assaf Elisabete M. , Assaf José M. , Gomes Janaina F. 
Published: February 2024

AbstractAlcohol production from CO2 hydrogenation is a cutting‐edge process in sustainable chemistry that holds vast promise for addressing climate change by recycling and repurposing emissions. Many strategies have been proposed to improve the process efficiency. In‐situ generated, and trace amounts of water added to the feed stream have recently proved to be determinant to promote key reaction steps, increasing alcohol selectivity and yield. Here, we discuss the main findings that led to an atomic‐level understanding of water promotional effects in CO2 hydrogenation to alcohols. H2O and the products resultant from its dissociation (OH and O) can act in different ways, stabilizing intermediates and active sites or participating in the hydrogen transfer mechanisms during the reaction. Gaining insights into the mechanisms underlying water promotion offers a cost‐effective strategy for enhancing alcohol production efficiency.
Diversity Oriented Synthesis of Novel Xanthones Reveal Potent Doxorubicin‐Inspired Analogs
Authors: Meringdal Jonas W. , Bade Leon , Bendas Gerd , Menche Dirk 
Published: February 2024

AbstractInspired by potent antiproliferative xanthone natural products and so far limited examples of derived bioactive agents, a structure activity study of architecturally novel types of xanthones is reported. Their preparation was enabled in a short and divergent manner by a modular chlorination in combination with optimized protocols for a polar condensation and a hetero‐cyclization. Application of these procedures allowed for the synthesis of various polyhalogenated representatives (including mixed bromo/chloro xanthones) that were obtained in up to fourfold improved yields as compared to previous procedures. Subsequent Suzuki coupling of either halide enabled access to phenyl‐ and chloro‐bearing xanthones, which may be functionalized at four out of five non‐hydroxylated positions. Antiproliferative assays against breast cancer cell lines revealed potent activities of some of these simplified analogs that are in the range of pharmaceutically used anticancer drug doxorubicin.
Abscopal effect in maxillary sinus cancer: Insights from two case reports and a literature review
Authors: Sakai Akihiro , Ebisumoto Koji , Iijima Hiroaki , Yamauchi Mayu , Maki Daisuke , Fukuzawa Tsuyoshi , Okami Kenji 
Published: February 2024

AbstractBackgroundThe abscopal effect is a rare phenomenon in which localized radiation therapy triggers tumor reduction in nontargeted areas. Although this phenomenon has been observed in various cancer types, it remains infrequent and not fully understood.CaseTwo patients with maxillary sinus cancer with distant metastases were treated with radiotherapy after immune checkpoint inhibitor (ICI) therapy. The patients demonstrated abscopal effects following ICI therapy and radiotherapy, showing shrinkage in metastatic areas not directly targeted by radiation.ConclusionThis report was reviewed to examine the synergistic effects of ICI and radiotherapy and to identify optimal strategies to enhance the abscopal effect in clinical practice. It has also touched on various ongoing debates and clinical trials aimed at understanding and exploiting this effect to improve cancer treatment. The exact mechanisms and optimal treatment protocols remain areas for future research.
Mechanistic Inquisition on the Reduction of C17Si(NH2)2 to NH3: A DFT Study
Authors: Sen Sobitri , Bag Arijit , Pal Sourav 
Published: February 2024

AbstractActivation of molecular nitrogen by silicon‐substituted cyclo[18]carbon and its ability to produce the C17Si‐(NH2)2 derivative, as the precursor of NH3, has been recently reported. This specific acquisition has piqued an interest to investigate the possibility of NH3 formation with further addition of H2 molecules in the gaseous reaction media. The current investigations reported in this article show that two moles of molecular H2 generate two molecules of NH3 and a C17Si‐H2 byproduct from its precursor. The catalyst gets restored by an in situ reaction between some unreacted C17Si‐N2 and the byproduct in the media. This reaction also produces the next C17Si‐(NH)2 adduct, which restarts the catalytic cycle for NH3 production again.
Analytical Workflows for Single‐Cell Multiomic Data Using the BD Rhapsody Platform
Authors: Li Wenyan , Bazaz Sajad Razavi , Mayoh Chelsea , Salomon Robert 
Published: February 2024

AbstractThe conversion of raw sequencing reads to biologically relevant data in high‐throughput single‐cell RNA sequencing experiments is a complex and involved process. Drawing meaning from thousands of individual cells to provide biological insight requires ensuring not only that the data are of the highest quality but also that the signal can be separated from noise. In this article, we describe a detailed analytical workflow, including six pipelines, that allows high‐quality data analysis in single‐cell multiomics. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Image analysisBasic Protocol 2: Sequencing quality control and generation of a gene expression matrixBasic Protocol 3: Gene expression matrix data pre‐processing and analysisBasic Protocol 4: Advanced analysisBasic Protocol 5: Conversion to flow cytometry standard (FCS) formatBasic Protocol 6: Visualization using graphical interfaces
Developing CSR communication strategies to increase sustainable behaviors from the perspective of spiritual capital
Authors: Lee Ya‐Ching 
Published: February 2024

AbstractMany companies pursue sustainability and rely on communication to convince consumers of their sustainability efforts. This study examines the impact of corporate sustainable communication strategies from the perspective of spiritual capital. Results indicate that communication framed in strength, personal well‐being, and mobilizability positively affects consumers' trust and sustainable behavior intentions through perceived spirituality. This study also clarifies gender differences. It offers a simple yet rigorous theoretical framework to evaluate sustainable communication strategies. The conceptualization and development of these measures is an important step for future studies that evaluate sustainable communication effects. To our knowledge, this study is the first attempt to explore the dimensions of perceived spirituality and evaluate their roles in sustainable communication. It finally offers practical suggestions for business sustainable communication strategies.
ADB‐HEXINACA—a novel synthetic cannabinoid with a hexyl substituent: phase I metabolism in authentic urine samples, a case report and prevalence on the German market
Authors: Giorgetti Arianna , Zschiesche Annette , Groth Olwen , Haschimi Belal , Scheu Martin , Pelletti Guido , Fais Paolo , Musshoff Frank , Auwärter Volker 
Published: February 2024

AbstractSynthetic cannabinoid receptor agonists (SCRAs) are one of the largest groups of new psychoactive substances (NPS). Yet, another novel analog started spreading on the NPS market around 2021. Soon after, the substance could be analytically characterized in herbal material as ADB‐HEXINACA, an SCRA containing a hexyl‐substituted tail on the indazole core. Here, we present suitable urinary markers to prove the consumption of this analog, a case report of acute polydrug intoxication and data on its prevalence in Germany. Anticipated phase I metabolites were detected in 12 authentic urine samples that were collected for abstinence control and analyzed by ultra‐performance liquid chromatography coupled to a time‐of‐flight mass spectrometer (UPLC‐qToF‐MS). The results of in vivo samples were confirmed by analysis of in vitro incubates with pooled human liver microsomes (pHLMs). Forensic samples were used to assess the prevalence of ADB‐HEXINACA. Thirty‐two phase I metabolites were detected in the authentic urine samples. The main metabolites resulted from amide hydrolysis in combination with either monohydroxylation or ketone formation at the hexyl moiety (M15 and M26), the monitoring of which is recommended as a proof of consumption. ADB‐HEXINACA was detected in 3.5% of SCRA positive urine samples collected for abstinence control in Freiburg up to December 2022 and in 5.5% of the SCRA positive blood/serum samples. The hexyl substituent of ADB‐HEXINACA allows for the detection of specific urinary biomarkers suggested as analytical targets to confirm its prior intake. ADB‐HEXINACA had a rather low prevalence in Germany, alternating months of higher prevalence with periods of total absence.
Sustaining eastern oak forests: Synergistic effects of fire and topography on vegetation and fuels
Authors: Hutchinson Todd F. , Adams Bryce T. , Dickinson Matthew B. , Heckel Maryjane , Royo Alejandro A. , Thomas‐Van Gundy Melissa A. 
Published: February 2024

AbstractAcross much of the eastern United States, oak forests are undergoing mesophication as shade‐tolerant competitors become more abundant and suppress oak regeneration. Given the historical role of anthropogenic surface fires in promoting oak dominance, prescribed fire has become important in efforts to reverse mesophication and sustain oaks. In 2000 we established the Ohio Hills Fire and Fire Surrogate (FFS) study to examine whether repeated prescribed fire (Fire), mechanical partial harvest (Mech), and their combined application (Mech + Fire) reduced the dominance of subcanopy mesophytic competitors, increased the abundance of large oak–hickory advance regeneration, created a more diverse and productive ground‐layer flora, and produced fuel beds more conducive to prescribed fire, reducing the risk of high‐severity wildfire. Here we report on the ~20‐year effects of treatments on vegetation and fuels and examine the support for interactive effects across a topographic‐moisture and energy gradient. In general, we found that Fire and Mech + Fire treatments tended to reverse mesophication while the Mech‐only treatment did not. The moderate and occasionally high‐intensity fires resulted in effects that were ultimately very similar between the two fire treatments but were modulated by topography with increasing fire severity on drier sites. In particular, we found support for an interaction effect between treatment and topography on forest structure and tree regeneration responses. Fire generally reduced mesophytic tree density in the midstory and sapling strata across all site conditions, while leading to substantial gains in the abundance of large oak–hickory advance regeneration on dry and intermediate landscape positions. Fire also promoted ground‐layer diversity and created compositionally distinct communities across all site conditions, primarily through the increased richness of native perennial herbs. However, the fire had limited effects on fine surface fuel loading and increased the loading of large woody fuels, potentially increasing the risk of high‐severity wildfire during drought conditions. We conclude that two decades of repeated fires, with and without mechanical density reduction, significantly shifted the trajectory of mesophication across most of the landscape, particularly on dry and intermediate sites, highlighting the capacity of a periodic fire regime to sustain eastern oak forests and promote plant diversity but modulated by topography.
Differentiation between atypical anorexia nervosa and anorexia nervosa using machine learning
Authors: Sandoval‐Araujo Luis E. , Cusack Claire E. , Ralph‐Nearman Christina , Glatt Sofie , Han Yuchen , Bryan Jeffrey , Hooper Madison A. , Karem Andrew , Levinson Cheri A. 
Published: February 2024

AbstractObjectiveBody mass index (BMI) is the primary criterion differentiating anorexia nervosa (AN) and atypical anorexia nervosa despite prior literature indicating few differences between disorders. Machine learning (ML) classification provides us an efficient means of accurately distinguishing between two meaningful classes given any number of features. The aim of the present study was to determine if ML algorithms can accurately distinguish AN and atypical AN given an ensemble of features excluding BMI, and if not, if the inclusion of BMI enables ML to accurately classify between the two.MethodsUsing an aggregate sample from seven studies consisting of individuals with AN and atypical AN who completed baseline questionnaires (N = 448), we used logistic regression, decision tree, and random forest ML classification models each trained on two datasets, one containing demographic, eating disorder, and comorbid features without BMI, and one retaining all features and BMI.ResultsModel performance for all algorithms trained with BMI as a feature was deemed acceptable (mean accuracy = 74.98%, mean area under the receiving operating characteristics curve [AUC] = 74.75%), whereas model performance diminished without BMI (mean accuracy = 59.37%, mean AUC = 59.98%).DiscussionModel performance was acceptable, but not strong, if BMI was included as a feature; no other features meaningfully improved classification. When BMI was excluded, ML algorithms performed poorly at classifying cases of AN and atypical AN when considering other demographic and clinical characteristics. Results suggest a reconceptualization of atypical AN should be considered.Public SignificanceThere is a growing debate about the differences between anorexia nervosa and atypical anorexia nervosa as their diagnostic differentiation relies on BMI despite being similar otherwise. We aimed to see if machine learning could distinguish between the two disorders and found accurate classification only if BMI was used as a feature. This finding calls into question the need to differentiate between the two disorders.
Integrating context in the examination of pubertal timing effects on disordered eating among Black and Latinx girls
Authors: Anaya Carolina 
Published: February 2024

AbstractEarlier pubertal onset has been associated with increased disordered eating symptoms in cisgender girls. Although this finding has been replicated across studies of disordered eating, most studies have focused on white samples. The lack of studies is surprising given that early pubertal timing may impact disordered eating risk in Black and Latinx girls differently due to trends of earlier pubertal onset in these groups and increased stressors related to interpersonal and structural racism and economic marginalization. Current methods of examining pubertal timing among Black and Latinx girls may also not fully capture their experience. Contextual factors (e.g., neighborhood and school racial/ethnic composition) may influence how minoritized girls both perceive and are affected by their pubertal timing. Moreover, factors such as ethnic–racial identity development and experiences of discrimination may be important mechanisms explaining the association between pubertal timing and disordered eating risk. This paper aims to provide a brief overview of studies examining pubertal timing and disordered eating risk among Black and Latinx girls in the US and to discuss recommendations for future research that integrate contextual factors in the examination of pubertal timing and its effects.Public significanceEarly pubertal timing has been associated with increased risk of disordered eating symptoms among cisgender girls; however, studies have been limited due to the use of predominantly white samples. The article provides a brief overview of findings related to pubertal timing effects among Black and Latinx girls, discusses considerations for the measurement of pubertal timing, and highlights the need for inclusion of contextual factors in future research.
Synthesis conditions affecting electrochemical and chemical stabilities of Ga‐doped Li7La3Zr2O12 solid electrolyte
Authors: Huang DingYuan , Kamiko Masao , Yagi Shunsuke 
Published: February 2024

AbstractAll‐solid‐state lithium batteries with Li metal anodes and solid‐state electrolytes (SSEs) can achieve higher energy density and enhanced safety compared to the current liquid‐based Li‐ion batteries. Among several SSEs, Li7La3Zr2O12 (LLZO) has attracted attention due to its high Li+ ion conductivity (∼10−3 S cm−1 at room temperature for Ga‐doped LLZO) and good stability in ambient air. However, the challenges of Li penetration and the chemical instability against Li are the primary obstacles to its practical application. This study investigates the effects of the grain size and electronic conductivity of Ga‐doped LLZO on the critical current density (CCD). Using samples with similar interfacial impedances between Ga‐doped LLZO and Li, we demonstrate that a decrease in the grain size of Ga‐doped LLZO lowers the electronic conductivity, leading to a higher CCD. Furthermore, although a previous study suggests that Ga‐doped LLZO might be unsuitable for direct contact with Li, the chemical stability against Li is enhanced in a more compact pellet prepared at a higher cold‐pressing pressure. These results underscore the significance of the sintering conditions and pellet pressing pressure in the synthesis of Ga‐doped LLZO since they ultimately affect the electrochemical and chemical stabilities of the Ga‐doped LLZO solid electrolyte with a Li‐metal anode.
Season‐specific impacts of climate change on canopy‐forming seaweed communities
Authors: Truong Anthony T. , Edwards Matthew S. , Long Jeremy D. 
Published: February 2024

AbstractUnderstory assemblages associated with canopy‐forming species such as trees, kelps, and rockweeds should respond strongly to climate stressors due to strong canopy‐understory interactions. Climate change can directly and indirectly modify these assemblages, particularly during more stressful seasons and climate scenarios. However, fully understanding the seasonal impacts of different climate conditions on canopy‐reliant assemblages is difficult due to a continued emphasis on studying single‐species responses to a single future climate scenario during a single season. To examine these emergent effects, we used mesocosm experiments to expose seaweed assemblages associated with the canopy‐forming golden rockweed, Silvetia compressa, to elevated temperature and pCO2 conditions reflecting two projected greenhouse emission scenarios (RCP 2.6 [low] & RCP 4.5 [moderate]). Assemblages were grown in the presence and absence of Silvetia, and in two seasons. Relative to ambient conditions, predicted climate scenarios generally suppressed Silvetia biomass and photosynthetic efficiency. However, these effects varied seasonally—both future scenarios reduced Silvetia biomass in summer, but only the moderate scenario did so in winter. These reductions shifted the assemblage, with more extreme shifts occurring in summer. Contrarily, future scenarios did not shift assemblages within Silvetia Absent treatments, suggesting that climate primarily affected assemblages indirectly through changes in Silvetia. Mesocosm experiments were coupled with a field Silvetia removal experiment to simulate the effects of climate‐mediated Silvetia loss on natural assemblages. Consistent with the mesocosm experiment, Silvetia loss resulted in season‐specific assemblage shifts, with weaker effects observed in winter. Together, our study supports the hypotheses that climate‐mediated changes to canopy‐forming species can indirectly affect the associated assemblage, and that these effects vary seasonally. Such seasonality is important to consider as it may provide periods of recovery when conditions are less stressful, especially if we can reduce the severity of future climate scenarios.