bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact Of Connective Tissue Matrix Products and Critical Size Defects on Gut Microbiota and Fracture Healing in Mice
Authors: Harris Alexander , Morris Ashlyn , Varner Will , Parker Reggie , Nazzal Murad , Creecy Amy , Karnik Sonali J. , Blosser Rachel J. , Scott Elizabeth , Wang Hannah , Margetts Tyler , Dragisic Marko , Ganguly Upasana , Fehrenbacher Jill C. , White Fletcher A. , Hathaway-Schrader Jessica , Kacena Melissa A. 
Published: January 2024

Abstract:
Approximately 6.2 million people in the USA alone suffer from some form of fracture annually. Depending on the intensity of trauma and complexity of the fractures, some fractures will not heal without medical intervention. It is imperative to develop novel therapies that target fracture healing. Connective Tissue Matrix (CTM) Biomedical is a company that develops human placenta, amnion, chorion, and other umbilical derived allograft products which may provide a solution to the problem of impaired fracture healing. CTM products contain structural proteins, cytokines, and growth factors that may have regenerative and anti-inflammatory properties. Although these products are already used clinically for fracture and wound healing in humans, no pre-clinical studies exist verifying their efficacy or mechanism of action.
Commensal microbiota is a collection of microorganisms such as bacteria, fungi, and viruses. Disruption of the gut microbiota, known as gut dysbiosis, can lead to a variety of disorders in other tissues. In fact, new studies indicate that alterations in the gut microbiota can affect bone health. This is likely due to impaired nutrient uptake and an increase in inflammation from bacterial byproducts that are not favorable to a healthy gut ecosystem. More recent studies indicate that the microbiota is also implicated in fracture healing.
Our study aims to investigate if CTM products have a positive effect on fracture healing by affecting the gut microbiota composition and bacteriome. To test this, critical sized defects (CSD) were induced in mice and treated with various CTM implants or saline control. Fecal samples were taken on the day of surgery and weekly thereafter. Bacterial DNA was subsequently extracted from these samples and analyzed using PCR. We anticipate seeing changes in load and composition of gut bacteria following CSD surgery.
Coil-Assisted Retrograde Transvenous Obliteration (CARTO) for the Treatment of Gastric Variceal Bleeding in a Patient with a Gastrorenal Shunt: A Case Report
Authors: Bolda Ryan , Yedavalli Ramana , Han Amy 
Published: January 2024

Background
Portal hypertension (pHTN) is primarily caused by liver cirrhosis, which can lead to varices at portosystemic anastomoses.1,2 Gastric variceal bleeding (GVB) is rare but can be life-threatening with a mortality up to 50% for each bleeding episode. When endoscopic banding is ineffective and TIPS placement is contra-indicated due to risk of post-TIPS encephalopathy, coil-assisted retrograde transvenous obliteration (CARTO), which has shown great success in some studies, can be an effective treatment.5,8,9,10 This case demonstrates successful use of CARTO to treat GVB in a patient with a gastrorenal shunt (GRS) who presented with pHTN.

Case Overview
A 62-year-old female with cirrhosis due to nonalcoholic steatohepatitis (NASH) presented with pHTN and GVB. The patient had a massive GRS, which permitted access through the systemic venous circulation to treat the bleeding varices. Furthermore, the patient had hepatic encephalopathy (HE), making TIPS placement a poor choice due to risk of HE exacerbation. CARTO was performed to treat the bleeding varices and had the added benefit of decreasing risk of HE.

Discussion/Results
This case highlights CARTO in a patient with a 22mm GRS using a dual catheter system. Eighteen detachable 035 coils were first deployed in the GRS, followed by complete occlusion with a Gelfoam slurry to obliterate the gastric varices. Studies have described CARTO, all of which have shown incredibly high technical (complete embolization by angiography and/or CT) and clinical (no variceal rebleeding) success rates. This patient returned for follow-up CT and endoscopic imaging, which confirmed technically and clinically successful CARTO.

Conclusion and Potential Impact
CARTO appears to be a safe alternative to TIPS when treating GVB caused by pHTN, especially in patients who have HE and a GRS. Thus, CARTO should be discussed as a treatment option for these patients. Although portal pressure increases post-CARTO, the improvement of HE can lead to an improved safety profile with future TIPS.
Implantation of Flexible Electrodes for Simultaneous in-vivo Extracellular Recording and Two-Photon Imaging
Authors: Booth Alec , Khan Hammad , Kolhe Om , Jayant Krishna 
Published: January 2024

Introduction: Rigid silicon electrodes like Utah array grids and Neuropixel probes have been used in human and animal brain models to understand the dynamics of neural computation, treat neurodegenerative disorders, and act as brain-machine-interfaces. However, when implanted chronically, glial proliferation can rapidly disrupt the interaction between neurons and electrodes, drastically reducing recording fidelity. The development of flexible electrodes has the potential to minimize tissue damage and inflammation, which allows for long-term recordings over several months. In line with this objective, the Nano-neurotechnology Lab at Purdue University has developed a 6-µm thick, flexible, and biocompatible Parylene probe to facilitate chronic recordings in awake mice. However, flexible electrodes present a unique engineering challenge as the force required to insert into the brain causes the probe to buckle and fail during insertion.
Methods and Results: Here, I designed a micropipette shuttle using a glass micropipette and custom insertion system which provided reproducible probe implantation into the cortex. The implantation device was designed in CAD software and 3D-printed for rapid prototyping. The procedure was developed on brain phantoms made of 0.6% agarose with a comparable Young’s modulus to mouse brain tissue. Utilizing 3D-printed pieces and the surface tension of diluted poly-vinyl-acrylate adhesive to align the probe to a micropipette, insertion of the electrode and retraction of the shuttle was accomplished in awake mice.
Conclusion: The implications of flexible recording electrodes are extensive. Long-term implantation opens the door for understanding behavioral and learning dynamics over time. Moreover, the flexibility of these probes allows for the combination of 2-photon optical microscopy, thus enabling multi-modal investigation of neuronal physiology. A low-cost, consistent procedure is the first step in the implementation of these flexible probes for further advancements in fundamental neuroscience research and its potential applications in human and animal studies.
The Effects of Integrative Palliative Oncology on Health System Burden
Authors: Brandon Jackson , McCollom Joseph 
Published: January 2024

Background: Early integration of palliative care in oncology has been shown to benefit patients on an individual level, especially regarding mood and quality of life. These patients both have a difficult disease course – where palliative care can help with interpersonal, spiritual, physical, and care planning concerns – and represent a substantial burden on inpatient facilities like intensive care units and emergency departments. This study explores relationships between early palliative oncology, patient healthcare experience, and broader burden on the health system.
Methods: A retrospective chart review was performed comparing oncology patients at Parkview Regional Medical Center in Fort Wayne, IN. Cohort A (200 patients) received palliative care along with standard oncology care while cohort B (200 patients) received standard oncology care alone. Post-diagnosis emergency department visits, inpatient stays, and intensive care unit stays were compared. So were in-hospital deaths, referrals to hospice, and record of advance care planning documents.
Results: Two endpoints evaluated by Chi2 analysis were statistically significant (p values <0.01): Cohort A was more likely than cohort B to be referred to hospice (79.5% vs 31.5%) and more likely to have advance care planning documents on file (38.5% vs 21%). Additionally, a T-test showed statistically significant difference (p=.001) for inpatient stays post-palliative care encounter versus patients who had no palliative care (1.75 vs 2.41).
Conclusion: This study shows correlation between integrated oncology care with patients filing ACP documents and being referred to hospice. Additionally, it finds that cancer patients who visit the palliative care office have fewer inpatient stays after their visit than those who don’t visit at all.
Future Implications: Future studies in this area should explore the latter finding from new perspectives, perhaps focusing on the differences between palliative care visit promptly after diagnosis/staging and palliative care implemented long after diagnosis/staging or not administered at all.
Length of Stay Index – A Retrospective Chart Review on In-patient Dermatology Consults
Authors: Cahya Cynthia , Newton Edita 
Published: January 2024

Background and Objective: In monitoring inpatient care quality and efficiency, one metric of interest is Length of Stay (LOS) by diagnosis-related group (DRG) system and how this compares to peer institutions. Vizient Inc. provides a source of such benchmarking - collecting and analyzing individual case-level data from community and academic medical center hospitals nationwide. Using this data, Vizient calculates an expected LOS by DRGs and adjusts for severity of illness and other factors for inter-hospital comparison. Because LOS reduction is an important mechanism for improving outcomes and efficiency of care, The Department of Dermatology would like to understand which dermatologic consults are exceeding the Vizient expected LOS (LOS index > 1).
Project Methods: Consecutive charts of 663 patients with dermatology-related diagnoses in the inpatient setting at IU University and Methodist Hospitals between January 2021 and January 2023 were reviewed. Parameters identified included: category of dermatologic disorder, LOS index, time to consult, and quarter of encounter. Data was organized and analyzed using paired t-tests, linear regression, and descriptive statistics in JMP software.
Results: The mean LOS index for Dermatology inpatient consults was 1.89 (n=469). Patients whose dermatologic diagnosis fell under chronic wound, mechanical, infectious, drug adverse event, autoimmune, neoplasm, inflammatory, vascular, and blistering had a significant increase in observed length of stay compared to expected length of stay (p < 0.05). Time to dermatology consult was not found to correlate to shortening or prolonging LOS index.
Potential Impact: The data from this review will help direct the Department of Dermatology to the dermatologic consults with the highest need for potential interventions.
Changes in Transport Mode for Pediatric Trauma Patients Before and After the COVID-19 Pandemic
Authors: An Byoungchan , Raymond Jodi L. , Landman Matthew Paul 
Published: January 2024

Background: The Emergency Medical Services (EMS) system has faced significant stress due to the COVID-19 pandemic and now workforce issues. This study was performed to evaluate potential changes in pediatric trauma patient transport over that time period. We hypothesized that more trauma patients would use private transportation/personal vehicles to arrive at the ED in the post-pandemic time frame when compared pre-pandemic.
Methods: This was a retrospective cohort study of patients that were admitted to the Emergency Department of Riley Hospital for Children between 01/01/2017 to 12/31/2022. Patients were excluded if they were transferred from another hospital or had mechanisms of injury including burns, suffocations, drownings, hangings, medical, and ingestions. Pre- and post-COVID patients were defined as admission to the ED before or after March 25, 2020, the day after a stay at home orders were implemented in Indiana. Univariate and multivariate analyses were performed.
Results: A total of 4,116 patients matching the criteria were identified with 52% arriving after the start of the COVID-19 shut down orders. 30.6% of patients arrived by private operated vehicles (POV) during pre-covid times and 30.3% in the post-covid years (p=0;39). A logistic regression analysis was performed for mortality which demonstrated that transport mode (ground ambulance vs helicopter vs POV) was not statistically associated with mortality rate (OR 0.36, 95% CI 0.12 – 1.10, p=0.07), when controlling for important clinical variables associated with injury severity.
Conclusion: There was no statistical difference in arrival by POV before and after the COVID-19 pandemic in our cohort. Additionally, there was no significant data to suggest that transport mode was associated with mortality. Further research should be done to assess potential barriers to transport to pediatric trauma centers, particularly considering ongoing EMS workforce strains.
Importance of Per2 in Cardiac Mitochondrial Function during Stress
Authors: Bhaskara Meghana , Anjorin Olufisayo , Yoniles Arris , Liu Jean , Wang Meijing 
Published: January 2024

Background and Hypothesis: Ischemic heart disease is the worldwide leading cause of death. Cardiac cellular damage from ischemia is mainly inflicted in the form of mitochondrial dysfunction by inflammatory cytokines and reactive oxidative species (ROS). Mitochondria are critical for metabolic function to maintain cardiac activity. Interventions against inflammatory cytokines and ROS are therefore cardioprotective during ischemic damage. Period Circadian Regulator 2 (Per2) is a circadian rhythm protein involved in metabolic regulation as a time-responsive gene in cardiomyocytes during ischemic damage. Overexpression of Per2 has been shown to decrease infarct size following myocardial infarction. In this study, we hypothesize that Per2 protein plays a regulatory role in the mitochondrial response to inflammatory cytokine TNFα and oxidative stressor H2O2 in human cardiomyocytes.
Project Methods: AC16 Human Cardiomyocytes (HCM) transfected with Per2 or control siRNA were subjected to stress treatment of 100ng/mL TNFa or 100μM H2O2. RT-PCR and Western blot were used to detect Per2 expression. After two hours of treatment, mitochondrial membrane potential ([Equation]M) was detected using JC1 fluorescence probe and mitochondrial respiration capacity was evaluated via Seahorse Mito Stress Test. After four hours of treatment, cell death was measured using Annexin V and propidium iodide (PI) apoptosis kit via flow cytometry.
Results: Per2 siRNA significantly reduced Per2 mRNA and protein levels in HCM. Increased cell death and decreased [Equation]M were observed in HCM treated with TNFa or H2O2. Knockdown of Per2 potentiated TNFa-induced cell death, TNFa- or H2O2 -disrupted [Equation]M, and TNFa- or H2O2- impaired mitochondrial maximal respiration.
Conclusion and Potential Impact: Per2 knockdown increases apoptotic susceptibility and mitochondrial dysfunction in human cardiomyocytes exposed to TNFa or H2O2. Delivery of Per2 may serve as a promising therapeutic strategy to protect cardiomyocyte mitochondrial function during periods of stress, such as myocardial infarction, organ transplantation, and cardiac surgery.
NARRATIVA HISPANOAMERICANA CONTEMPORÁNEA (1960 - 1980)
Authors: Huarag Eduardo 
Published: January 2024

Narrativa hispanoamericana contemporánea (1960 - 1980).

En esta investigación planteamos que el boom de la narrativa hispanoamericana supuso el manejo de nuevas técnicas narrativas y la consiguiente renovación en el modo de contar. El novelista asume su alejamiento del realismo social, mimético. Entiende que lo literario es un ficcional autónomo, con intención metonímica en el desarrollo de la trama narrativa. El corpus elegido, en esta oportunidad, toma como referencia las innovaciones logradas por Cortázar, Fuentes, Vargas Llosa y García Márquez. Los escritores coinciden en su voluntad innovador, pero no pertenecen a un movimiento literario. Además, como se va a demostrar, las innovaciones son distintas, como diferentes son los estilos de cada novelista.
GESTÃO DE CONFLITOS SOCIOAMBIENTAIS EM UNIDADE DE CONSERVAÇÃO AMAZÔNICA: UMA EXPERIÊNCIA A PARTIR DO TERMO DE COMPROMISSO
Authors: Lima Tatiane Rodrigues , De Aguiar Cavalcante Maria Madalena , Geminiano De Melo Iranira , Mascarenhas Barbosa Rodrigues Marcos 
Published: January 2024

Neste artigo, analisa-se o processo de gestão de conflitos implementado no Parque Nacional Mapinguari, junto à Comunidade Tradicional Castanheiros do Rio Umari, município de Lábrea, estado do Amazonas, Região Norte do Brasil. Com categoria de proteção integral, por vezes, os parques nacionais são criados em sobreposição às áreas de uso tradicional de povos isolados na Amazônia, gerando conflitos socioambientais. O caso em tela apresenta aspectos relevantes para a discussão, visto que é reconhecida a importância do Parque Nacional Mapinguari para conter o avanço do Arco do Desmatamento na fronteira Rondônia/Amazonas, uma vez que, como medida de proteção de recursos naturais, o parque contou com o Projeto Ação Sustentável (PAS) do Programa Áreas Protegidas da Amazônia/ARPA, desenvolvido como estratégia de gestão com foco na capacitação em produção sustentável e educação ambiental como possível caminho de sustentabilidade econômica. Neste sentido, somou-se à ação, a construção coletiva de termos de compromisso como instrumento normativo de consolidação do ordenamento territorial na região, assegurando o acesso da comunidade aos castanhais.
Identification of Kappaphycus alvarezii seaweed based on phylogenetic and carrageenan conten
Authors: Satriani Gloria Ika , Tri Soelistyowati Dinar , Alimuddin  , Arfah Harton , Effendi Irzal 
Published: January 2024

Increasing seaweed production requires accurate information regarding the genetic sources of seeds used. Identifying the seaweed species Kappaphycus molecular is one of the solutions to ensure seaweed cultivators choose seeds for their cultivation businesses. Molecular identification is essential for the system traceability of seaweed products and the creation of databases regarding species variant information Kappaphycus alvarezii cultivation as potential data collection for developing and genetically breeding seaweed seeds. To date, there is no information on the genetic potential of K. alvarezii cultivated in various seaweed cultivation centers in Indonesia. This study aimed to obtain phylogenetic details based on identification of the genetic source using DNA molecular markers barcoding rbcL and analysis of carrageenan content using the Fourier transform infra-red (FTIR) spectrum. The results of DNA sequencing analysis and FTIR testing of 16 varieties of seaweed seedlings obtained from various cultivation centers in Indonesia showed 99% similarity with K. alvarezii, a producer of kappa carrageenan.

Keywords: DNA sequencing, phylogenetics, haplotypes, kappa-carrageenan, rbcL

ABSTRAK

Peningkatan produksi rumput laut memerlukan informasi yang akurat mengenai kepastian sumber genetik bibit yang digunakan. Identifikasi spesies rumput laut Kappaphycus secara molekuler merupakan salah satu solusi untuk memberikan kepastian pada pembudidaya rumput laut untuk memilih bibit bagi usaha budidaya. Identifikasi molekuler sangat penting dalam sistem traceability produk rumput laut dan pembuatan basis data mengenai informasi varian spesies Kappaphycus alvarezii budidaya sebagai pendataan potensi untuk pengembangan dan pemuliaan bibit rumput laut secara genetis. Sampai saat ini belum tersedia informasi mengenai potensi genetik rumput laut K. alvarezii yang dibudidayakan di berbagai sentra budidaya rumput laut di Indonesia. Penelitian ini bertujuan untuk memperoleh informasi filogenetik berdasarkan identifikasi sumber genetiknya menggunakan penanda molekuler DNA barcoding rbcL serta analisis kandungan karaginannya menggunakan spektrum Fourier Transform Infra-Red (FTIR). Dari hasil analisis sekuensing DNA dan pengujian FTIR terhadap 16 varietas bibit rumput laut yang diperoleh di berbagai sentra budidaya di Indonesia menghasilkan 99% kemiripan yang tinggi dengan K. alvarezii penghasil kappa karagenan.

Kata kunci: DNA sekuensing, filogenetik, haplotipe, kappa-karagenan, rbcL
Optimization of SVC Placement and Capacity in the Electric Power System Transmission Networks using Multi-Objective Improved Sine Cosine Algorithm
Authors: Abdillah Muhammad , Maulana Ferbyansyah Gilang , Nugroho Teguh Aryo 
Published: January 2024

Current technological developments are in line with the increasing consumption of electrical energy. There is a value of power losses of the electricity transmission process caused by an increase in the value of power losses, to overcome this, SVC (Static VAR Compensator) of the Flexible AC Transmission System (FACTS) can be used. From previous studies, the optimization of SVC placement in the transmission network has not been carried out to get better power losses. This research uses the Improved-Sine Cosine Algorithm (ISCA) that has a different function of r1 compared to the ordinary SCA, in which the use of the ISCA method is able to overcome the weaknesses of the SCA method. The determination of location and capacity can use more than one objective function. From the result, the optimization of SVC placement and capacity is able to reduce the value of power losses by up to 85%.
Fabrication of pH-responsive dextran- modified polylactic acid core-shell microparticles for antidiabetic drug delivery.
Authors: Kumar Sumit , Bansal Smriti , Singh Ankita , Poddar Deepak , Sarkar Anjana 
Published: January 2024

Abstract
Glipizide, a low-cost antidiabetic drug, known for its fast-acting nature and reduced risk of hypoglycemia. However, its usage is limited by its short acting nature and non-selectivity. In our study we demonstrate modified dextran coated glipizide loaded PLA (Polylactic acid) microspheres capable of targeted drug release at intestinal region. These particles show improved mucoadhesion in presence of glycidyltrimethyl ammonium chloride modified dextran (GT-dextran) as an outer coating. To achieve this glipizide encapsulated PLA, microspheres of size 27.09 ± 6.55 µm were fabricated using emulsion solvent evaporation followed by GT-dextran surface coating using dipping method. NMR, FT-IR, FESEM, TGA, and Zeta Potential confirmed the presence of GT-dextran coating on the surface of PLA particles. Controlled release of glipizide from the PLA matrix was achieved for the fabricated polymer particles. GT-dextran coating increases the size of the GI-PLA particles by 14.01 % with size 17.17 ±1.33 µm, along with 57.47 ± 2.7% encapsulation efficiency. The release behavior was assessed at three different pH, demonstrating slow release at of 8.76 ± 0.93 % low pH (1.5) and fast release of 92.4 ± 7.32 % at pH 7.2. The desired controlled release profile was achieved, with 68.45 ± 11.01% at pH 9.2 over a time of 48-hours. The fabricated pH-responsive core-shell type microspheres exhibit great potential as a targeted and controlled drug delivery system for antidiabetic therapy.