bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moracin E and M isolated from Morus alba Linné induced the skeletal muscle cell proliferation via PI3K-Akt-mTOR signaling pathway
Authors: Kwak Hee Jae , Kim Jinyoung , Kim Seo‐Young , Park SeonJu , Choi Junjeong , Kim Seung Hyun 
Published: November 2023

AbstractTwigs of Morus alba have been used in traditional medicine to treat muscle-related symptoms such as aches, numbness, and stiffness. Despite its clinical use in traditional medicine, its active compounds and mode of action have not yet been investigated. Therefore, we aimed to isolate the compounds from the twigs of M. alba and deduce active compounds, key gene targets, and mechanism of action against sarcopenia using network pharmacology analysis. Using various isolation techniques and spectroscopic methods, 43 phytochemicals, including 3 new flavonoids, were isolated and performed network pharmacology analysis. According to the computational-assistant analysis, 28 compounds, 9 genes, and the PI3K-Akt-mTOR signaling pathway were deduced as expected active compounds (EAC), key targets, and the main signaling pathway. To verify the predicted results, the cell proliferation activities of the EAC were evaluated. Especially, moracin E and M significantly increased by 130% (p < 0.001) and 57% (p < 0.05), respectively, which have more than 2- and 1.5-fold stronger effects compared to the control. Furthermore, both increased the expression level of proteins involved in the PI3K-Akt-mTOR signaling pathway and myogenic proteins, including myogenin and MyoD. This study demonstrated that moracin E and M exhibit cell proliferative effects on skeletal muscle cells through the PI3K-Akt-mTOR signaling pathway.
Hurricane risk assessment in a multi-hazard context for Dominica in the Caribbean
Authors: Sammonds Peter , Alam Akhtar , Day Simon , Stavrianaki Katerina , Kelman Ilan 
Published: November 2023

AbstractHurricanes can trigger widespread landslides and flooding creating compound hazards and multiple risks for vulnerable populations. An example is the island of Dominica in the Caribbean, where the population lives predominantly along the coast close to sea level and is subject to storm surge, with steep topography rising behind, with a propensity for landslides and flash river flooding. The simultaneous occurrence of the multiple hazards amplifies their impacts and couples with physical and social vulnerabilities to threaten lives, livelihoods, and the environment. Neglecting compound hazards underestimates overall risk. Using a whole island macroscale, (level-I) analysis, susceptibility scenarios for hurricanes, triggered landslides, and floods were developed by incorporating physical process parameters. The susceptibilities were combined with vulnerability indicators to map spatial patterns of hurricane multi-risks in Dominica. The analysis adopted a coupled approach involving the frequency ratio (FR), analytic hierarchy process (AHP), and geographic information system (GIS). Detailed hazard modelling was done at selected sites (level-II), incorporating storm surge estimates, landslide runout simulations, and steady flow analysis for floods. High-resolution terrain data and simulation models, the Rapid Mass Movement Simulation (RAMMS) and the hydrologic engineering center’s river analysis system (HEC-RAS), were employed. Ground validation confirmed reasonable agreement between projected and observed scenarios across different spatial scales. Following the United Nations Office for disaster risk reduction (UNDRR) call for the inclusion of local, traditional, and indigenous knowledge, feedback, and expert opinion to improve understanding of disaster risk, 17 interviews with local experts and 4 participatory workshops with residents were conducted, and findings were incorporated into the analysis, so as to gain insights into risk perceptions. The study’s outcomes encompass projections and quantification of hurricane compound hazards, vulnerabilities, accumulated risks, and an understanding of local priorities. These findings will inform decision-making processes for risk mitigation choices and community actions by providing a new framework for multi-hazard risk assessment that is easy to implement in combining different data forms.
A conceptual design of circular adaptive façade module for reuse
Authors: Niazy Dalia , Metwally Esraa A. , Rifat Mostafa , Awad Mohammed Ibrahim , Elsabbagh Ahmed 
Published: November 2023

AbstractClimate change has an impact on the ecosystem, and subsequently, it affects the built environment. Building envelope has a vital role in controlling the integration between indoor and outdoor environmental quality. The responsivity of the façade has proven its efficiency in optimizing the global energy performance of buildings. Adaptive façades are multifunctional reconciling envelope dynamic systems that improve sustainability with the purpose of utilizing environmental parameters. This paper tackles the research gap in integrating façades circularity, adaptive envelopes, and design for disassembly. The research investigates the merge between biodegradability, circularity of adaptive façades components, and interior space micro-climate control for energy efficiency. This paper presents a proof of concept for a circular adaptive façade during two phases in its life cycle: operation and reuse phases. A scientific quantitative method took place which is based on a hybrid method; computational simulation, smart control, and an up-scale model. Adaptability is investigated through the façade life cycle from design to disassembly instead of demolition and consequent waste production, by exploiting sustainable materials. As a result, an empirical prototype is constructed. The prototype provides 3 levels of adaptability across the design, operation, and disassembly for reuse. Subsequently, this work proposes an up-scale physical model that can help in mitigating the climate change effects.
Data-driven cranial suture growth model enables predicting phenotypes of craniosynostosis
Authors: Liu Jiawei , Froelicher Joseph H. , French Brooke , Linguraru Marius George , Porras Antonio R. 
Published: November 2023

AbstractWe present the first data-driven pediatric model that explains cranial sutural growth in the pediatric population. We segmented the cranial bones in the neurocranium from the cross-sectional CT images of 2068 normative subjects (age 0–10 years), and we used a 2D manifold-based cranial representation to establish local anatomical correspondences between subjects guided by the location of the cranial sutures. We designed a diffeomorphic spatiotemporal model of cranial bone development as a function of local sutural growth rates, and we inferred its parameters statistically from our cross-sectional dataset. We used the constructed model to predict growth for 51 independent normative patients who had longitudinal images. Moreover, we used our model to simulate the phenotypes of single suture craniosynostosis, which we compared to the observations from 212 patients. We also evaluated the accuracy predicting personalized cranial growth for 10 patients with craniosynostosis who had pre-surgical longitudinal images. Unlike existing statistical and simulation methods, our model was inferred from real image observations, explains cranial bone expansion and displacement as a consequence of sutural growth and it can simulate craniosynostosis. This pediatric cranial suture growth model constitutes a necessary tool to study abnormal development in the presence of cranial suture pathology.
Exploring potential therapeutic agents for lipopolysaccharide-induced septic cardiomyopathy based on transcriptomics using bioinformatics
Authors: Feng Shaodan , Cai Kexin , Lin Siming , Chen Xiaojun , Luo Yuqing , Wang Jing , Lian Guili , Lin Zhihong , Xie Liangdi 
Published: November 2023

AbstractSeptic cardiomyopathy (SCM) is a common and severe complication of sepsis, characterized by left ventricular dilation and reduced ejection fraction leading to heart failure. The pathogenesis of SCM remains unclear. Understanding the SCM pathogenesis is essential in the search for effective therapeutic agents for SCM. This study was to investigate the pathophysiology of SCM and explore new therapeutic drugs by bioinformatics. An SCM rat model was established by injection of 10 mg/kg lipopolysaccharide (LPS) for 24 h, and the myocardial tissues were collected for RNA sequencing. The differentially expressed genes (DEGs) between LPS rats and control (Ctrl) with the thresholds of |log2fold change|≥ 1 and P < 0.05. A protein–protein interaction (PPI) network was constructed based on the DEGs. The hub genes were identified using five algorithms of Cytoscape in the PPI networks and validated in the GSE185754 dataset and by RT-qPCR. The hub genes were analyzed by Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG), as well as Gene set enrichment analyses (GSEA). In addition, the miRNAs of hub genes were predicted through miRWalk, and the candidate therapeutic drugs were identified using the Connectivity Map (CMAP) database. This study revealed the identified hub genes (Itgb1, Il1b, Rac2, Vegfa) and key miRNAs (rno-miR-541-5p, rno-miR-487b-3p, rno-miR-1224, rno-miR-378a-5p, rno-miR-6334, and rno-miR-466b-5p), which were potential biological targets and biomarkers of SCM. Anomalies in cytokine-cytokine receptor interactions, complement and coagulation cascades, chemokine signaling pathways, and MAPK signaling pathways also played vital roles in SCM pathogenesis. Two high-confidence candidate compounds (KU-0063794 and dasatinib) were identified from the CMAP database as new therapeutic drugs for SCM. In summary, these four identified hub genes and enrichment pathways may hold promise for diagnosing and treating SCM.
The central role of climate action in achieving the United Nations' Sustainable Development Goals
Authors: Filho Walter Leal , Wall Tony , Salvia Amanda Lange , Dinis Maria Alzira Pimenta , Mifsud Mark 
Published: November 2023

AbstractSustainable Development Goal (SDG) 13 refers to “Climate Action”. It is one of the 17 goals established by the United Nations in their 2030 Agenda for Sustainable Development. The primary objective of SDG13 is to take urgent action to combat climate change and its impacts. It recognises that climate change is a global challenge that requires immediate attention and concerted efforts from governments, businesses, communities, and individuals worldwide. SDG13 permeates a number of SDGs and also influences them in a significant way. Based on the need to contextualise SDG13 and considering its role as one of the central SDGs, this article outlines the links between SDG13 and the other SDGs. It also reports on a survey involving experts from 61 countries. The findings suggest that even though climate change impacts, particularly extreme weather events, are known to disproportionally affect poorer and minoritized communities, the synergies among related goals and climate justice seem to receive less attention. The article concludes by describing some of the means via which synergies between SDG13 and other SDGs may be achieved.
Selection of metallic liquid in sub-6 GHz antenna design for 6G networks
Authors: Dash Sasmita , Psomas Constantinos , Krikidis Ioannis 
Published: November 2023

AbstractThe rapid evolution of wireless communication systems toward 6G demands efficient antennas with the features of adaptability and versatility. Liquid antennas have gained significant research interest due to their unique features in realizing small, flexible, transparent, and reconfigurable antennas for promising applications in future wireless systems. In this paper, in order to find a suitable metallic liquid for effective antennas, we design and compare the performance of metallic liquid antennas using Mercury, gallium indium alloy (EGaIn), and Graphene metallic liquid in the sub-6 GHz frequency. The antenna is realized by the metallic liquid in a poly methyl methacrylate microfluidic channel over a liquid crystal polymer substrate at 5.6 GHz frequency. The performance of these metallic liquid antennas is analyzed by their electromagnetic and radiation performance. The Graphene-based metallic liquid antenna shows better electromagnetic performance in comparison to Mercury and EGaIn metallic liquid antennas.
Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting properties
Authors: Kajander Karoliina , Sirkiä Saara V. , Vallittu Pekka K. , Heino Terhi J. , Määttä Jorma A. 
Published: November 2023

AbstractDifferent biomaterials have been clinically used as bone filling materials, although the mechanisms behind the biological effects are incompletely understood. To address this, we compared the effects of five different biomaterials: two bioactive glasses (45S5 and S53P4), hydroxyapatite (HAP), carbonated apatite (CAP), and alumina on the in vitro migration and viability of pre-osteoblastic cells. In addition, we studied the effects of biomaterials’ calcium release on cell migration, viability and differentiation. We found differences between the materials as the bioactive glasses promoted rapid pre-osteoblastic cell migration. In contrast, CAP decreased cell migration, which was also associated with lower activity of migration related kinases. Bioactive glasses released significant amounts of calcium into the media, while CAP decreased the calcium concentration. The response of cells to calcium was mechanistically studied by blocking calcium sensing receptor (CaSR) and ATP-gated ion channel P2X7, but this had no effect on cell migration. Surprisingly, HAP and CAP initially decreased cell viability. In summary, bioactive glasses 45S5 and S53P4 had significant and long-lasting effects on the pre-osteoblastic cell migration, which could be related to the observed calcium dissolution. Additionally, bioactive glasses had no negative effects on cell viability, which was observed with HAP and CAP.
Characterization of gut microbiome composition in Iranian patients with nonalcoholic fatty liver disease and nonalcoholic steatohepatitis
Authors: Abdollahiyan Sara , Nabavi-Rad Ali , Keshavarz Azizi Raftar Shahrbanoo , Monnoye Magali , Salarieh Naghmeh , Farahanie Azam , Asadzadeh Aghdaei Hamid , Zali Mohammad Reza , Hatami Behzad , Gérard Philippe , Yadegar Abbas 
Published: November 2023

AbstractGut microbiota dysbiosis is intimately associated with development of non-alcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Nevertheless, the gut microbial community during the course of NAFLD and NASH is yet to be comprehensively profiled. This study evaluated alterations in fecal microbiota composition in Iranian patients with NAFLD and NASH compared with healthy individuals. This cross-sectional study enrolled 15 NAFLD, 15 NASH patients, and 20 healthy controls, and their clinical parameters were examined. The taxonomic composition of the fecal microbiota was determined by sequencing the V3-V4 region of 16S rRNA genes of stool samples. Compared to the healthy controls, NAFLD and NASH patients presented reduced bacterial diversity and richness. We noticed a reduction in the relative abundance of Bacteroidota and a promotion in the relative abundance of Proteobacteria in NAFLD and NASH patients. L-histidine degradation I pathway, pyridoxal 5'-phosphate biosynthesis I pathway, and superpathway of pyridoxal 5'-phosphate biosynthesis and salvage were more abundant in NAFLD patients than in healthy individuals. This study examined fecal microbiota dysbiosis in NAFLD and NASH patients and presented consistent results to European countries. These condition- and ethnicity-specific data could provide different diagnostic signatures and therapeutic targets.
Mine pressure behavior law of isolated island working face under extremely close goaf in shallow coal seam
Authors: Lan Tianwei , Liu Yonghao , Yuan Yongnian , Liu Hongliang , Liu Hongguang , Zhang Shunfeng , Wang Shunxiang 
Published: November 2023

AbstractIn order to study the mining pressure characteristics of the shallow buried coal seam with the same silo working face under the very close mining void zone and the overlying coal rock body, the theoretical analysis is used to determine whether the open-cutting eye bearing layer belongs to the mining under the very close mining void zone or not, based on the numerical simulation of FLAC3D and on-site measurement of the working resistance at the end of the cycle of the working face's hydraulic bracket, It is proposed to divide the mining stress of the working face based on the advancing length of the working face, that is, the high-pressure zone, the transition zone and the low-pressure zone. The results of the study show that: FLAC3D software was used to analyze the stress intensity of the “C” island working face when it was mined back to 50 m, 100 m, 150 m, and 180 m (one time “square”), and the simulation results were imported into the Origin software, which was used to analyze the stress intensity of the working face. The simulation results were imported into Origin software, and the influence range of mining stress was divided into four areas: high-stress area, stress transition area, low-stress area, and "square" stress concentration area. According to the on-site measurement of the working resistance at the end of hydraulic support cycle, the initial pressure step of the working face under the overlying coal rock body is 48.9–55.7 m, with the peak value of 38 MPa, the cycle pressure step is 9.0–23.3 m, with the peak value of 36 MPa, and the dynamic load factor of the working face is 1.14–1.16; relative to the overlying coal rock body, the average decrease of the cycle pressure step is nearly 10% and the average increase of dynamic load factor is 1.14–1.16; compared with that under the overlying coal rock body, the average decrease of the cycle pressure step is nearly 10% and the average increase of dynamic load factor is 1.14–1.16. Compared with the overlying coal rock body, the average decrease of the cycle pressure step under the overlying mining zone is nearly 10%, the average increase of the dynamic load factor is 20%, and there is no obvious regularity and periodicity in the direction of strike, and the working face is divided into three parts along the direction of strike: high-pressure zone, transition zone, and low-pressure zone. Therefore, in the process of mining under the overlying coal rock body, we should strengthen the roadway peripheral rock support and roof and floor management, which is of guiding significance to the mining of similar working faces and mine safety production.
A compact SiGe D-band power amplifier for scalable photonic-enabled phased antenna arrays
Authors: Broucke Reinier , Singh Nishant , Van Osta Michiel , Van Kerrebrouck Joris , Demeester Piet , Lemey Sam , Torfs Guy 
Published: November 2023

AbstractTo address the rising demand for high-speed wireless data links, communication systems operating at frequencies beyond $${100}\,\hbox {GHz}$$

100

GHz

are being targeted. A key enabling technology in the development of these wireless systems is the phased antenna array. Yet, the design and implementation of such steerable antenna arrays at frequencies over $${100}\,\hbox {GHz}$$

100

GHz

comes with a multitude of challenges. In particular, the cointegration of active electronics at each antenna element poses a major hurdle due to the inherent space constraints in the array. This article proposes a novel scalable concept for opto-electronic phased antenna arrays operating at 140 GHz. It details the system architecture of a transmitter that enables the implementation of large scale, wideband, 2D steerable phased antenna arrays and presents the design and measurement of a compact SiGe power amplifier (PA) chip to be used as one of its key building blocks. The amplifier achieves a gain of 20 dB at 135 GHz, features a $$P_{1dB}$$

P

1
d
B

of 14.6 dBm and can support data rates up to 45 Gbps in a limited footprint of only 540μm × 550μm. This makes it one of the fastest, most powerful D-band power amplifiers in literature with a footprint compatible with $$\frac{\lambda }{2}$$

λ
2

-spaced phased array integration.
Irrigation level and substrate type on the acclimatization and development of mandacaru (Cereus jamacaru DC.): an emblematic cactus from Brazilian semiarid region
Authors: Cassimiro Carlos Alberto Lins , Henschel Juliane Maciel , Gomes Vanessa Gabrielle Nóbrega , Alves Rita de Cássia , da Silva Pollyana Karla , Pereira Emmanuel Moreira , Cavalcanti Mônica Tejo , Batista Diego Silva , da Costa Batista Fabiane Rabelo 
Published: November 2023

AbstractMandacaru is a cactus with great socioeconomic potential, but lack of information about its cultivation hinders its domestication. Here, we aimed to evaluate the acclimatization and vegetative development of mandacaru under different substrates and irrigation levels. For this, seeds inoculated in vitro were grown for 120 days, being transplanted to pots containing four types of substrate (S1—caatinga soil + gravel; S2—washed sand + organic matter + soil + charcoal; S3—washed sand + cattle manure + soil + sand; S4—commercial organic substrate). Pots were irrigated with 100% of the field capacity (FC) once-a-week, or with 50% FC twice-a-week, and kept in a greenhouse for six months. The experimental design was completely randomized, in a 4 × 2 factorial scheme, with six replications. Plant height and diameter, axial and radial growth rate, fresh and dry mass of stem and root, water content, and photosynthetic pigments were determined. Growth was affected mainly by the substrate, with S4 resulting in higher growth and pigment content, while S1 was impaired and S2 and S3 resulted in intermediate growth. The use of S4 and 100% FC once per week was the best condition for mandacaru.
Access to thioethers from thiols and alcohols via homogeneous and heterogeneous catalysis
Authors: Markwitz Martyna , Labrzycki Klaudiusz , Azcune Laura , Landa Aitor , Kuciński Krzysztof 
Published: November 2023

AbstractA metal-free dehydrative thioetherification method has been reported, enabling the conversion of various alcohols and thiols into thioethers. By employing triflic acid as a catalyst or utilizing a recyclable NAFION® superacid catalyst, these methods significantly improve the efficiency and practicality of sulfide preparation.
Long-term HIF-1α stabilization reduces respiration, promotes mitophagy, and results in retinal cell death
Authors: Nsiah Nana Yaa , Morgan Autumn B. , Donkor Nina , Inman Denise M. 
Published: November 2023

AbstractOcular hypertension during glaucoma can lead to hypoxia, activation of the HIF transcription factors, and a metabolic shift toward glycolysis. This study aims to test whether chronic HIF activation and the attendant metabolic reprogramming can initiate glaucoma-associated pathology independently of ocular hypertension. HIF-1α stabilization was induced in mice for 2 and 4 weeks by inhibiting prolyl hydroxylases using the small molecule Roxadustat. HIF-1α stabilization and the expression of its downstream bioenergetic targets were investigated in the retina by immunofluorescence, capillary electrophoresis, and biochemical enzyme activity assays. Roxadustat dosing resulted in significant stabilization of HIF-1α in the retina by 4 weeks, and upregulation in glycolysis-associated proteins (GLUT3, PDK-1) and enzyme activity in both neurons and glia. Accordingly, succinate dehydrogenase, mitochondrial marker MTCO1, and citrate synthase activity were significantly decreased at 4 weeks, while mitophagy was significantly increased. TUNEL assay showed significant apoptosis of cells in the retina, and PERG amplitude was significantly decreased with 4 weeks of HIF-1α stabilization. A significant increase in AMPK activation and glial hypertrophy, concomitant with decreases in retinal ganglion cell function and inner retina cell death suggests that chronic HIF-1α stabilization alone is detrimental to retina metabolic homeostasis and cellular survival.