bluuebunny/crossref_metadata_2025_split huggingface dataset:
#194 : Predictive Value of Serum Kisspeptin in Early First Trimester Pregnancy During IVF Treatment: A Prospective Study
Authors: Li He 
Published: January 2024

Background and Aims: Kisspeptin is an emerging biomarker for the discrimination of viable pregnancy. The aim of the study is to determine whether serum kisspeptin can predict the first trimester miscarriage and compare it with serum hCG in the prediction of the first trimester miscarriage. Method: This study is a prospective case-control design including 178 women who had experienced early miscarriage (n = 21) and viable single pregnancy (n = 157), following frozen-thawed transfer (FET) from May 2019 to December 2019. Serum samples on 14 days, 21 days and 28 days after FET were collected for kisspeptin and hCG detection. Trial registration number: NCT03940495 Results: On day 21 after FET, serum kisspeptin levels were significantly lower in the early miscarriage group [0.260(0.185-0.375)] vs in the viable single pregnancy group [0.370(0.280–0.495)] (p=0.005). Similar results were showed on day 28 after FET, the serum kisspeptin levels were significantly lower in the early miscarriage group [0.27(0.2–0.33)] vs in the viable single pregnancy group [0.670(0.455–1.235)] (p<0.001). But on day 14 after FET, serum kisspeptin levels were comparable in the early miscarriage group [0.260(0.210–0.325)] and in the viable single pregnancy group [0.280(0.215–0.340)] (p=0.551). Serum kisspeptin levels on day 21 and 28 have a poor predictive value of miscarriage compared with serum hCG levels. [Day 21: area under the curve = 0.687 (kisspeptin) and 0.816 (hCG); Day 28: area under the curve = 0.896 (kisspeptin) and 0.909 (hCG)]. Conclusion: Serum kisspeptin on day 14 failed to discriminate between miscarriage and ongoing pregnancies, and on day 21, 28 has a poor predictive value of miscarriage. Funding: This study was supported by the Special Project for Clinical Research in the Health Industry of the Shanghai Municipal Health Commission (20194Y0036).
#207 : Mendelian Randomization Study Reveals a Casual Effect of Blood Lipids and Apolipoprotein on Uterine Fibroids
Authors: Liu Yanxu 
Published: January 2024

Background and Aims: Despite an early insight into the risk factors of uterine fibroids (UFs), there is still controversy about the roles of blood lipids and apolipoprotein on UFs. Therefore, to provide more reliable evidence, we investigated the causal effect of blood lipids and apolipoprotein on the risk of UFs through Mendelian randomization (MR). Method: We utilized the large genome-wide association studies of blood lipids and apolipoprotein as instruments and integrated them in the inverse-variance-weighted (IVW) model in two-sample MR to investigate their causal relationship in large sample sizes. To eliminate potential pleiotropy, we listed a conclusive table of genes related to confounding factors, such as body mass index, waist circumference, blood pressure, glucose, hypertension, the metabolic syndrome and smoking and removed the repeated SNPs in the exposure data.To adjust for multiple comparisons, the statistical significance of the MR effect estimates was evaluated in a false discovery rate correlation of less than 5%. Neither pleiotropy nor heterogeneity was reported in all MR sensitivity analysis, including IVW, MR-Egger, simple median, weighted median, weighted mode and Mendelian Randomization Pleiotropy Residual Sum and Outlier methods. Results: Total triglycerides, very low-density lipoprotein (VLDL) cholesterol, including large, medium and small VLDL particles and free cholesterol in large VLDL cholesterol showed positive association with UFs. Meanwhile, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1, free cholesterol in small and medium VLDL and total cholesterol are not statistically related to the development of UFs. Conclusion: Exposure to various aberrant blood lipids increases the risk of UFs, providing genetic evidence for the vital role of aberrant lipid levels in the pathogenesis of UFs. Meanwhile, our results provided genetic insights suggesting intervention in blood lipids might be a prospective strategy in the early prevention and treatment of UFs.
#346 : A 5-Year Retrospective Cross-Sectional Study on theAssociation of Obesity with Myoma Uteri, Endometrial Polyps, Adenomyosis and Polycystic Ovarian Syndrome
Authors: Defensor Pamela Grace 
Published: January 2024

Background and Aims: To assess the association of obesity with myoma uteri, endometrial polyp, adenomyosis and polycystic ovary syndrome (PCOS) Method: A retrospective cross-sectional study was performed on a cohort of 586 women consulting at the outpatient department of a tertiary government institution from 2016 to 2020. Age-adjusted logistic regression models were used to assess the association of obesity with the diseases. Results: No significant association between obesity and myoma uteri (p:0.91), endometrial polyp (p:0.49) and adenomyosis (p:0.56) were observed. A direct association between obesity and PCOS (p<0.01) was observed wherein obese women were five times more likely to have PCOS than non-obese women, regardless of age. Conclusion: There is no association between obesity and myoma uteri, endometrial polyp and adenomyosis in obese Filipino women. Age seems to have a more direct association in the presence of these diseases. There is a direct association of obesity and polycystic ovary syndrome.
#382 : Congenital Adrenal Hyperplasia with Concomitant Mullerian Agenesis: A Rare Occurance in 46 XX Disorders of Sexual Development (DSD)
Authors: Wahyuni Zulfia , Yuad Haviz 
Published: January 2024

Background and Aims: The most common cause of 46 XX DSD is due to congenital adrenal hyperplasia with varying degree of virilization. Females with CAH do not produce anti mullerian hormone (AMH), therefore the internal genitalia, which are mullerian derivatives, are develop normally. We report a unique case of CAH with absent uterus, cervix, and fallopian tube. Method: The clinical, laboratory, and imaging findings of the patient are presented with literature review. Results: We report an ongoing case of a 20-year-old patient with ambiguous genitalia and primary amenorrhea. She was born from a healthy mother and raised as a female. There was no family history of the same disorder. On physical examination we found that she had normal height, normal blood pressure, palatoschizis, undeveloped breast (Tanner stage 1), and clitoral virilization with single urovaginal opening (Prader stage 3). We performed a G-Banded chromosome analysis, with 46 XX results. Laboratory test showed her 17-hidroxyprogesterone level was 176,93 ng/ml, FSH level was 3,06 mIU/ml, LH level was 2,21 mIU/ml, and estradiol level was 18,0 pg/mL. Ultrasound imaging revealed the absence of uterus, cervix, and vagina, as well as abnormal appearance of ovarium. We have planned to perform a complete MRI study for other anomalies as we suspected this patient had Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome type II. Conclusion: In the presence of external genitalia and urinary tract malformations in patients with CAH, it is important to investigate for possible associated mullerian anomalies. A comprehensive approach in diagnosis could lead to a better outcome in a patient’s treatment and wellbeing.
#6 : Effects of Co-Treatment of GnRH-Analog Alone or in Combination with Aromatase Inhibitor or Progestin on Endometrial - Integrin Expressions in Women with Recurrent Miscarriage
Authors: Ahmad Mohd Faizal 
Published: January 2024

Background and Aims: Recurrent miscarriage (RM) is postulated caused by endometrium receptivity. We aimed to assess the expression of ανβ3 integrin in endometrium tissue during the window of implantation (WOI) following GnRH analog alone or in combination with either aromatase inhibitor (AI) or progestin (PrG) to consolidate the outcome. Method: A randomized control trial (RCT) was done in Hospital Canselor Tuanku Muhriz (HCTM) UKM. Women with RM were divided into three groups - Group I-GnRHa, Group II–GnRHa + AI, or Group III–GnRa + PrG. The endometrial tissue biopsy was taken during the luteal phase (between LH+7 and LH+9), then repeated biopsy post-treatment. The ανβ3 integrin expression was evaluated for pre- and post-treatment. The intensity and distribution in endometrial glands were done using HSCORE system. Results: 39 women with RM were included with 13 in each group. The mean age was older in Group I; 38.00 ± 4.9 years old with mean BMI of 25.7 ± 2.44 kg/m2. Group II had significantly the highest intensity and distribution of ανβ3 integrin expression > 50 following the treatment; from 28.6% to 42.9% (p < 0.05), and an increase in HSCORE following the treatment from 1.82 ± 0.70 to 2.36 ± 0.56 (p < 0.05). Meanwhile, Group I HSCORE noted was elevated; from 1.90 ± 0.91 to 2.29 ± 0.77, and Group III was also elevated from 1.67 ± 0.77 to 1.90 ± 0.87. However, all were not statistically significant (p > 0.05). Conclusion: The ανβ3 integrin expression can be significantly elevated with GnRHa and AI as pre-treatment, thus aiming for better implantation results among women with RM.
#106 : The Efficacy of Intra-Ovarian Infusion of Autologous Platelet-Rich Plasma in Patients with Poor Ovarian Response: A Systematic Review and Meta-Analysis
Authors: Liu Jiajia , Gao Mingxia 
Published: January 2024

Background and Aims: Poor ovarian response (POR) is a pathological condition characterized by a reduced ovarian response to gonadotropin stimulation. This condition presents with a low number of developed follicles during the ovulation induction cycle, low blood estradiol (E2) peak, high gonadotropin dosage, fewer retrieved oocytes, increased cycle cancellation rate, and low clinical pregnancy rate. POR is a significant challenge in assisted reproductive technology. Autologous platelet-rich plasma (PRP) has emerged as a potential therapy for POR, but its efficacy remains controversial. This meta-analysis aims to determine the effect of intraovarian infusion of PRP on ovarian reserve and pregnancy outcomes in patients with POR. Methods: This study was designed as a Meta-analysis. Women diagnosed with POR based on POSEIDON or Bologna criteria were included. Primary outcomes included anti-Müllerian hormone (AMH), antral follicle count (AFC), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and E2 levels, while secondary outcomes were the number of retrieved oocytes and embryo quality. Results: Seven prospective cohort studies involving 202 participants were included. Intra-ovarian infusion of autologous PRP significantly increased AMH (SMD: 0.20, 95% CI: 0.05–0.36; P=0.01), AFC (SMD: 0.92, 95% CI: 0.59–1.25, P<0.05), and E2 (SMD: 3.46, 95% CI: 1.13–5.79; P=0.004) levels in patients with POR. PRP also decreased LH levels in POR (SMD: −1.17, 95% CI: −2.28 to −0.06; P=0.04). However, no statistically significant effect on FSH levels in POR was observed (SMD: −0.17, 95% CI: −0.78 to 0.44; P=0.59). Furthermore, PRP positively impacted the number of retrieved oocytes and embryo quality in IVF cycle patients. Conclusion: This meta-analysis indicates that intra-ovarian injection of autologous PRP can improve ovarian reserve and pregnancy outcomes in patients with POR.
#107 : Association Between COVID-19 Vaccination Status and Spontaneous Abortion in Pregnant Women
Authors: Simanjuntak Leo , Simanjuntak Benhard Christopher 
Published: January 2024

Background and Aims: Pregnant women are at greater risks for morbidity and mortality if infected by the COVID-19. Vaccination is critical in mitigating the risks. However, there is still limited data around safety profiles of COVID-19 vaccines in pregnant women especially in Indonesia. Therefore, this study is aimed to determine the association of COVID-19 vaccination status on the occurrence of spontaneous abortion in pregnant women in Indonesia. Method: This cross-sectional study was carried out from March-June 2022 at a private maternity clinic in Medan, Indonesia. Participants who agreed to participate were asked to sign the informed consent. Participants then were interviewed, and their diagnosis was confirmed using ultrasonography. Demographic data, vaccination status, parity, gestational age and the diagnosis were collected and statistically analyzed. The Chi-square test or Fisher’s exact test were done to analyze the categorical variables. Results: Of the 319 participants, 267 (84%) vaccinated and 52 (16%) unvaccinated. All the vaccinated participants received at least 1 dose to 3 doses of COVID-19 vaccine before pregnancy confirmation. There were 70 (22%) participants with spontaneous abortion and 249 (78%) with normal pregnancy. All our participants were having < 20 weeks of gestation, and majority were in reproductive age (82%) and primiparous (55%). Multiparity or gestational age were associated with spontaneous abortion occurrence (p < 0.05). We did not find association between pregnant women vaccination status and spontaneous abortion occurrence (p > 0.05). Conclusion: Our data indicated that COVID-19 vaccination history was not associated with spontaneous abortion occurrence, and thus, adding information regarding the safety of COVID-19 vaccination in pregnancy, especially in Indonesia.
#143 : Assessment of Ovarian Reserve in Infertile Women with Hypothyroidism
Authors: Shima Umme Tahmina 
Published: January 2024

Background: Impaired thyroid function may affect the follicular growth and development and reduces the number of follicles in ovaries. Thus, hypothyroidism may cause the reduction of ovarian reserve in women with reproductive age group. This study aimed to assess the ovarian reserve in infertile hypothyroid women. Methods: This cross-sectional study was conducted in Reproductive Endocrinology and Infertility Unit, Dhaka Medical College Hospital, Bangladesh from July 2021 to June 2022. Total 167 hypothyroid infertile women of 20–35 years of age who had S. TSH level >2.5 mIU/L with normal/low free T4 were included. Ovarian reserve was evaluated by serum anti-Mullerian hormone (AMH) and antral follicle count (AFC). Thyroid autoimmunity was also assessed by measuring anti thyroid per oxidase antibody (TPOAb) and anti-thyroglobulin antibody (TgAb). Then the relationship of ovarian reserve, thyroid hormones and anti-thyroid antibodies were assessed. Results: Among the 167 women with hypothyroidism, 124 (74.3%) patients had sub-clinical hypothyroidism (SCH) and rest 43 (25.7%) had overt hypothyroidism. Infertile women with overt hypothyroidism had significantly higher TPOAb(+) (67.4%) and TgAb (+) (46.5%) than those of subclinical hypothyroid women (TPOAb+ve : 29%, TgAb+ve : 29.8%, p-value: 0.014). Correlation analysis showed that there was significant negative correlation between AMH and TSH (r: −0.029, p-value: 0.024), TPOAb (r: −0.053, p value: 0.011), TgAb (r: −0.083, p-value: 0.018). Diminished ovarian reserve was significantly associated with overt hypothyroidism in this study population (p-value: 0.001). 33(26.6%) and 23(53.5%) of SCH and overt hypothyroid women had diminished ovarian reserve respectively. Conclusion: Anti-Mullerian hormone was negatively associated with increased TSH level and with anti-thyroid antibodies (TPOAb and TgAb). Diminished ovarian reserve was significantly associated overt hypothyroidism. So, the study findings suggest that hypothyroidism has a negative effect on ovarian reserve.
#196 : Office Hysteroscopy Results in Patients with Recurrent Implantation Failure at Tam Anh General Hospital
Authors: Vu Thi Ngoc , Thi Huyen La , Minh Thuy Nguyen , Phuc Hieu Nguyen , Quang Do Le , Thu Hanh Tran Thi , Le Hoang 
Published: January 2024

Background and Aims: To describe the clinical characteristics and evaluate the results of office hysteroscopy in patients with recurrent implantation failure at Tam Anh General Hospital. Methods: A descriptive study on 151 patients at Tam Anh General Hospital who had failed embryo transfer twice or more consecutively was carried out from January 2021 to March 2023. Results: 151 patients were included in the study, 41,1% of which had unexplained infertility. The mean age and duration of infertility were 34,5 ± 5,2 years and 5,7 ± 3,4 years, respectively. 55,0% of patients exhibited uterine cavity abnormalities during office hysteroscopy, with the most common is endometritis accounting for 29.8%. The median time to perform the procedure was 3 (2) minutes, the mean VAS pain score was 3,2 ± 1,1 points. No complications related to office hysteroscopy were noted. Conclusions: A office hysteroscopy was able to detect intrauterine pathologies which were missed by other investigative modalities. In individuals who have experienced repeated implantation failure, office hysteroscopy is a safe and practical approach that should be recognized as a crucial tool for intrauterine evaluation.
#276 : Impact of Previous Cesarean Delivery on Pregnancy and Neonatal Outcomes of Subsequent In Vitro Fertilization/Intracytoplasmic Sperm Microinjection and Single Frozen-Thawed Embryo Transfer
Authors: Cao Kexin , Zhang Qian , Li Jie , Liu Xinyu , Yan Yueyue , Zhou Wei , Ni Tianxiang , Yan Junhao 
Published: January 2024

Background and Aims: Cesarean delivery (CD) rates continue to rise worldwide. Prior studies have indicated that CD is associated with not only various gynaecological symptoms and obstetric complications but also lower probability of subsequent fertility. However, the recognition about impact of CD on clinical outcomes in subsequent IVF/ICSI-FET is very limited. The purpose was to investigate the effects of previous CD on pregnancy and neonatal outcomes in single frozen-thawed embryo transfer (FET) cycles so that women with a previous cesarean delivery can be given detailed fertility counseling during the ART process. Methods: This is a large retrospective cohort study including a total of 5,750 patients who desired to transfer single vitrified-thawed blastocyst from the same oocyte retrieval cycle as their last live birth between January 2011 and January 2021 in a center for reproductive medicine in Shandong, China, comprising FET cycles of 3,853 previous single CD and 1,897 previous single vaginal delivery (VD). Results: Women with a previous CD had significantly lower rates of biochemical pregnancy (64.52% vs. 71.48% before propensity score matching (PSM), P<0.001; and 65.58% vs. 71.49% after PSM, P<0.001), clinical pregnancy (58.16% vs. 65.79% before PSM, P<0.001; and 59.22% vs. 66.17% after PSM, P<0.001) and live birth (48.17% vs. 55.82% before PSM, P<0.001; and 49.29% vs. 56.10% after PSM, P<0.001) than those with a previous VD. Meanwhile, the CD rate was significantly higher in the CD group (97.85% vs. 18.53% before PSM, P<0.001; and 98.02% vs. 20.51% after PSM, P<0.001). The rates of multiple pregnancy, miscarriage, ectopic pregnancy, multiple live birth, labor complications and preterm and neonatal outcomes were comparable between the two groups. Conclusion: Compared to VD, a previous CD is associated with a significant reduction in the rates of biochemical pregnancy, clinical pregnancy and live birth in patients undergoing subsequent single frozen-thawed blastocyst transfer.
#335 : Ovarian Cortex with Higher Vascularization Density is Associated with Higher PTEN Expression After Transplantation on CAM
Authors: Ayuandari Sarrah , Dewanto Agung , Widad Shofwal 
Published: January 2024

Background and Aim: Before being applied on the clinical settings, the establishment of ovarian tissue cryopreservation and transplantation process should be optimum. The vascularization after transplant is depended on the ovarian tissue size, which influence the follicular recruitment. It may then affect the post-transplantation survival. Methods: On chorionic-allantoic membrane (CAM) of 8-days-old, fertilized eggs, goat ovarian tissue samples (Capra hircus) were split into pre- and post-transplantation groups. Each group was subsequently divided into 2.5×2.5×1, 5×5×1, and 10×10×1 mm size. Vascularization density and vascularization surface area was analysed and correlated with PTEN protein expressions. Results: Pre- and post-transplantation samples were taken from a total of 36 strips of ovarian tissue. The vascularization density was found to be the highest in the 2.5×2.5×1 mm transplantation samples compared to the pre-transplantation samples (9.54±3.2; 15.46±1.85; P<0,05). Meanwhile the vascularization surface area was also significantly higher after transplantation regardless the size of ovarian tissue (P<0,05). Interestingly, the vascularization surface area was observed to be significantly higher in the cortex area adhered to CAM compared to more distant than the CAM. To magnify the vascularization results, in the 2.5×2.5×1 mm transplantation sample, it was shown that the higher PTEN expression after transplantation. Concurrently in the 10×10×1 mm transplantation, PTEN protein expressions were significantly lower in primordial follicles after transplantation (P<0.05). These results were also mutual for total number of primordial follicles in 2.5×2.5×1 mm, which were higher after transplantation (mean rank 11.92±2.56) compared to the other size groups (10.88±1.48; 9.72±6.36). Conclusions: The most vascularized ovarian tissue samples were found to be 2.5×2.5×1 mm in size and it was found to express more PTEN in the primordial follicles. As a result, compared to the other sample sizes, it also showed the least post-transplantation decline in the number of primordial follicles, indicating less follicular burn-out than the group with larger tissue surface area.
#389 : Fertility Preservation During Chemotherapy Treatment by NAD+ Repletion in Mice
Authors: Gilchrist Robert , Ho Wing-Hong Jonathan , Marinova Maria B , Listijono Dave R , Bertoldo Michael J , Richani Dulama , Lynn-Jee Kim , Brown Amelia , Riepsamen Angelique H , Cabot Safaa , Bustamante Sonia , Zhong Ling , Selesniemi Kaisa , Wong Derek , Goss Dale M , Li Catherine , Araki Toshiyuki , Livingston David J , Turner Nigel , Sinclair David A , Walters Kirsty A , Homer Hayden A , Wu Lindsay E 
Published: January 2024

Background and Aims: Chemotherapy induced ovarian failure and infertility is an important concern in female cancer patients of reproductive age or younger, and non-invasive, pharmacological approaches to prevent chemotherapy induced infertility are urgently needed. Here we investigate whether pharmacological elevation of nicotinamide adenine dinucleotide (NAD+) ameliorates chemotherapy induced female infertility in mice. Method: 8-week-old C57BL6 female mice were treated +/- chemotherapy (doxorubicin, Dox; 10 mg/kg) and +/- nicotinamide mononucleotide (NMN; 200 mg/kg i.p. once and 2 g/L in drinking water on-going), an orally bioavailable metabolic precursor to NAD+. Effects on fertility were measured by impact on ovarian reserve and folliculogenesis, ovulation rates and breeding performance. Effects on the ovarian NAD+ metabolome were assessed by mass spectrometry. A potential adverse effect of NMN on the efficiency of chemotherapy was assessed using a xenograft model of mammary cancer. Results: NMN treatment did not prevent a decline in the ovarian reserve caused by chemotherapy but did maintain the health of the remaining primordial follicle and total follicle populations, leading to a restoration in oocyte yield in chemo-treated mice (Dox vs Dox+NMN; P<0.007), culminating in an increase in pups born/mating in chemo+NMN treated mice (P<0.05). Chemo caused ovarian NMN, NADP+ and NADPH depletion, and NADPH was restored by NMN, which likely contributes to Dox detoxification. Importantly, treatment of the breast cancer mouse model with NMN reduced tumour growth and did not impair the efficacy of chemotherapy drugs in vivo or in diverse cancer cell lines. Conclusion: Overall, these findings raise the possibility that NAD+ precursors could be a non-invasive strategy for maintaining ovarian function and fertility in cancer patients, with potential benefits in cancer therapy.