bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental controls of dark CO2 fixation in wetland microbiomes
Authors: Grüterich Luise , Woodhouse Jason Nicholas , Mueller Peter , Tiemann Amos , Ruscheweyh Hans-Jo-achim , Sunagawa Shinichi , Grossart Hans-Peter , Streit Wolfgang R. 
Published: January 2024

ABSTRACTRising atmospheric concentration of CO2is a major concern to society due to its global warming potential. In soils, CO2fixing microorganisms are preventing a part of the CO2from entering the atmosphere. Yet, the pathways behind dark CO2fixation are rarely studiedin situ. Here we examined the environmental controls on the abundance and expression of key genes involved in microbial CO2fixation in estuarine wetlands. A combined multi-omics approach incorporating metabarcoding, deep metagenomic and metatranscriptomic analyses confirmed that wetland microbiota harbor all six known CO2fixation pathways and that these pathways are transcribed at high frequencies along several environmental gradients, albeit at different levels depending on the environmental niche. Notably, the transcription of the key genes for the reductive tricarboxylic acid cycle (rTCA) and the Calvin cycle were favored by low salinity and O2rich niches high in organic matter, while the transcription of the key genes for the Wood-Ljungdahl pathway (WLP) and dicarboxylate/4-hydroxybutyrate cycle (DC/4-HB cycle) were favored by low O2niches poor in organic matter. Taxonomic assignment of transcripts implied that dark CO2fixation was mainly linked to few bacterial phyla, namely, Desulfobacterota, Gemmatimonadota, Methylomirabilota, Nitrospirota and Pseudomonadota.
Eukaryotic genomic data uncover an extensive host range of mirusviruses
Authors: Zhao Hongda , Meng Lingjie , Hikida Hiroyuki , Ogata Hiroyuki 
Published: January 2024

SummaryA recent marine metagenomic study has revealed the existence of a novel group of viruses designated mirusviruses, which are proposed to form an evolutionary link between two realms of double-stranded DNA viruses,VaridnaviriaandDuplodnaviria. Metagenomic data suggest that mirusviruses infect microeukaryotes in the photic layer of the ocean, but their host range remains largely unknown. In this study, we investigated the presence of mirusvirus marker genes in publicly available 1,901 eukaryotic genome assemblies, mainly derived from unicellular eukaryotes, to identify potential hosts of mirusviruses. Mirusvirus marker sequences were identified in 1,348 assemblies spanning 284 genera across eight supergroups of eukaryotes. The habitats of the putative mirusvirus hosts included not only marine but also other diverse environments. Among the major capsid protein (MCP) signals in the genome assemblies, we identified 85 sequences that showed high sequence and structural similarities to reference mirusvirus MCPs. A phylogenetic analysis of these sequences revealed their distant evolutionary relationships with the seven previously reported mirusvirus clades. Most of the scaffolds with these MCP sequences encoded multiple mirusvirus homologs, underscoring the impact of mirusviral infection on the evolution of the host genome. We also identified three circular mirusviral genomes within the genomic data of the oil producing thraustochytridSchizochytriumsp. and the endolithic green algaOstreobium quekettii. Overall, mirusviruses probably infect a wide spectrum of eukaryotes and are more diverse than previously reported.HighlightsMirusvirus signals detected in genomic data from eight eukaryotic supergroups.Habits of putative mirusvirus hosts not limited to marine environments.Major capsid sequences from these assemblies show new mirusviral lineages.Three circular mirusvirus genomes were identified.
N-acetylglucosamine supplementation fails to bypass the critical acetylation of glucosamine-6-phosphate required forToxoplasma gondiireplication and invasion
Authors: Alberione María Pía , González-Ruiz Víctor , Rudaz Serge , Soldati-Favre Dominique , Izquierdo Luis , Kloehn Joachim 
Published: January 2024

AbstractThe cell surface ofToxoplasma gondiiis rich in glycoconjugates which hold diverse and vital functions in the lytic cycle of this obligate intracellular parasite. Additionally, the cyst wall of bradyzoites, that shields the persistent form responsible for chronic infection from the immune system, is heavily glycosylated. Formation of glycoconjugates relies on activated sugar nucleotides, such as uridine diphosphateN-acetylglucosamine (UDP- GlcNAc). The Glucosamine-phosphate-N-acetyltransferase (GNA1) generatesN- acetylglucosamine-6-phosphate critical to produce UDP-GlcNAc. Here, we demonstrate that downregulation ofT. gondiiGNA1 results in a severe reduction of UDP-GlcNAc and a concomitant drop in glycosylphosphatidylinositol (GPI), leading to impairment of the parasite’s ability to invade and replicate in the host cell. Surprisingly, attempts to rescue this defect through exogenous GlcNAc supplementation fail to completely restore these essential functions. In depth metabolomic analyses elucidate diverse causes underlying the failed rescue: utilization of GlcNAc is inefficient under glucose-replete conditions and fails to restore UDP-GlcNAc levels in GNA1-depleted parasites. In contrast, GlcNAc- supplementation under glucose-deplete conditions fully restores UDP-GlcNAc levels but fails to rescue the defects associated with GNA1 depletion. Our results underscore the essentiality of GlcN6P acetylation in governingT. gondiireplication and invasion and highlight the potential of the evolutionary divergent GNA1 in Apicomplexa as a target for the development of much-needed new therapeutic strategies.
A Multiscale approach reveals the molecular architecture of the autoinhibited kinesin KIF5A
Authors: Carrington Glenn , Fatima Uzrama , Caramujo Ines , Lewis Tarek , Casas-Mao David , Peckham Michelle 
Published: January 2024

AbstractKinesin-1 is a microtubule motor that transports cellular cargo along microtubules. KIF5A is one of three kinesin-1 isoforms in humans, all of which are autoinhibited by an interaction between the motor and an IAK motif in the proximal region of the C-terminal tail. The C-terminal tail of KIF5A is ∼80 residues longer than the other two kinesin-1 isoforms (KIF5B and KIF5C) and it is unclear if it contributes to autoinhibition. Mutations in KIF5A cause neuronal diseases and could affect autoinhibition, as reported for a mutation that skips exon 27, altering its C-terminal sequence. Here, we combined negative-stain electron microscopy, crosslinking mass spectrometry (XL-MS) and AlphaFold2 structure prediction to determine the molecular architecture of the full-length autoinhibited KIF5A homodimer, in the absence of light chains. We show that KIF5A forms a compact, bent conformation, through a bend between coiled coils 2 and 3, around P687. XL-MS of WT KIF5A revealed extensive interactions between residues in the motor, between coiled-coil 1 and the motor, between coiled-coils 1 and 2, with coiled coils 3 and 4, and the proximal region of the C-terminal tail and the motor in the autoinhibited state, but not between the distal C-terminal region and the rest of the molecule. While negative stain electron microscopy of exon-27 KIF5A splice mutant showed the presence of autoinhibited molecules, XL-MS analysis suggested that its autoinhibited state is more labile. Our model offers a conceptual framework for understanding how mutations within the motor and stalk domain may affect motor activity.
Finback: a web-based data collection system at SSRF biological macromolecular crystallography beamlines
Authors: Yu Feng , Liu Ke , Zhou Huan , Li Minjun , Kong Huating , Zhang Kunhao , Wang Xingya , Wang Weiwei , Xu Qin , Pan Qiangyan , Wang Zhijun , Wang Qisheng 
Published: January 2024

An integrated computer software system for macromolecular crystallography (MX) data collection at the BL02U1 and BL10U2 beamlines of the Shanghai Synchrotron Radiation Facility is described. The system, Finback, implements a set of features designed for the automated MX beamlines, and is marked with a user-friendly web-based graphical user interface (GUI) for interactive data collection. The Finback client GUI can run on modern browsers and has been developed using several modern web technologies including WebSocket, WebGL, WebWorker and WebAssembly. Finback supports multiple concurrent sessions, so on-site and remote users can access the beamline simultaneously. Finback also cooperates with the deployed experimental data and information management system, the relevant experimental parameters and results are automatically deposited to a database.
A novel corrosion inhibitor based on a Schiff base for mild steel in 1M HCl: synthesis and anticorrosion study
Authors: Baran Aydın Evrim , Başaran Eyüp , Ateş Sevgi , Çakmak Reşit 
Published: January 2024

Purpose
The aim of this study was to investigate the activity of 4-((4-((2-hydroxyethyl)(methyl)amino)benzylidene) amino)-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one (HEMAP), a Schiff base synthesized and characterized for the first time, to the authors’ knowledge, as a novel inhibitor against corrosion of mild steel (MS) in hydrochloric acid solution.

Design/methodology/approach
HEMAP was characterized by some spectroscopic methods including High-Resolution Mass Spectrometry (HRMS), Proton Nuclear Magnetic Resonance (1H NMR), Carbon-13 (C13) nuclear magnetic resonance (13C NMR) and Fourier Transform Infrared Spectroscopy (FT-IR). Then, the inhibition efficiency of HEMAP on MS in a hydrochloric acid solution was investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). To explain the inhibition mechanism, the surface charge, adsorption isotherms and thermodynamic parameters of MS in the inhibitor solution were studied.

Findings
EIS tests displayed that the highest inhibition efficiency was calculated approximately as 99.5% for 5 × 10−2 M HEMAP in 1 M HCl solution. The adsorption of HEMAP on the MS surface was found to be compatible with the Langmuir model isotherm. The thermodynamic parameter results showed that the standard free energy of adsorption of HEMAP on the MS surface was found to be more chemical than physical.

Originality/value
This study is important in terms of demonstrating the performance of the first synthesized HEMAP molecule as an inhibitor against the corrosion of MS in acidic media. EIS tests displayed that the highest inhibition efficiency was calculated approximately as 99.5% for 5 × 10−2 M HEMAP in 1 M HCl solution.
How does sports e-commerce influence consumer behavior through short video live broadcast platforms? Attachment theory perspective
Authors: Jiao Shoukai , Wang Xianliang , Ma Chao , Deng Yiran 
Published: January 2024

PurposeBased on the “S-O-R” and attachment theories, this paper constructs a research model of the platform attribute factors of sports short video live e-commerce on consumers' psychological conditions, and explores how platform attributes affect consumer behavior through consumer attachment.Design/methodology/approachThe study carried out questionnaire survey through the “snowball” method, and a total of 422 valid questionnaires were collected. The paper uses SPSS 26.0 and AMOS 26.0 to adapt the data and model, and adopts the method of structural equation modeling for analysis.FindingsThe research results show that the interactivity, identity, personalization and entertainment of live sports e-commerce platforms can actively stimulate consumer attachment, and directly drive their consumption behavior through their attachment.Practical implicationsExamining the platform attributes and system functions of short video live broadcast e-commerce from the perspective of consumer attachment can help sports e-commerce understand consumers' needs and satisfaction with the functions provided by the platform. Through timely optimization and improvement of system functions, the platform will make the connection between consumers and e-commerce closer, thereby increasing consumer stickiness and promoting the vigorous development of sports e-commerce.Originality/valueThis study identified and defined the platform attributes contained in the sports live broadcast e-commerce platform, combined the S-O-R model with the attachment theory, expanded the analytical framework of the S-O-R theory and made contributions to the extension of the attachment theory.
How optimal trust–dependence nexus facilitates relational behavior in contractor–subcontractor collaboration: a configurational analysis
Authors: Wu Shaoyan , Liu Mengxiao , Zhao Duo , Cao Tingting 
Published: January 2024

PurposeAlthough trust is generally taken as a fundamental factor in influencing relational behavior in contractor–subcontractor collaboration, the determination of an optimal level of trust is still lacking. Trust with an optimal tipping point that matches dependence best is considered the optimal trust to improve relational behavior between general contractors and subcontractors. To fill the knowledge gap, this study explores how combinations of trust and dependence trigger relational behavior between general contractors and subcontractors through a configurational approach.Design/methodology/approachQuestionnaires were administered to 228 middle management and technical staff members of the general contractor. The data were analyzed using fuzzy-set qualitative comparative analysis (fsQCA), and the inductive analytic method allowed researchers to explore configurations of different dimensions and levels of dependence and trust.FindingsNecessity analysis results indicated that neither dependence nor trust was a necessary condition for facilitating relational behavior. Through sufficiency analysis, four configurations of optimal trust matched with dependence were identified in contractor–subcontractor collaboration. Even if contractors rely only on subcontractors for resources, the optimal trust between contractors and subcontractors should include both institution- and cognition-based trust. In the event that contractor–subcontractor collaboration involves relational dependence, both affect- and cognition-based trust are necessary for the optimal trust.Originality/valueThis study enhances existing research by delving deeper into a nuanced understanding of optimal trust in dependence scenarios, and enriches project governance theory by uncovering the internal transmission of relational governance. Practically, this study offers general contractors guidance on how to establish optimal trust strategies based on the dual dependence level with subcontractors, which can facilitate subcontractors' relational behavior, and ultimately improve contractor–subcontractor collaboration performance.
Increasing physical activity among Indonesian adolescents: a social marketing intervention reflection
Authors: Schmidtke David James , Nguyen Mai , Rundle-Thiele Sharyn 
Published: January 2024

Purpose
This paper aims to provide an overview of a social marketing intervention that aimed to increase physical activity (aligned to UN SDG 3) among adolescents in Bali, Indonesia.

Design/methodology/approach
Three sequential phases were followed to deliver the social marketing intervention. Phrase 1 (formative research) gained insights that guided a subsequent social marketing intervention. Phase 2 (pilot intervention) gathered preliminary results, to support the development of the final intervention. Phase 3 (intervention) evaluated the effectiveness of the two-month social marketing intervention.

Findings
The results from the intervention tested in this paper identified significant behaviour change in physical activity, demonstrating the effectiveness of the intervention. Furthermore, the paper identifies which intervention inputs contribute to behaviour change and which do not.

Research limitations/implications
This paper describes the outcomes from an eight-week pilot programme that aimed to increase rates of physical activity for Indonesian adolescents and provides early evidence of impact.

Practical implications
This study found that providing adolescents with the opportunity to play team sports increases physical activity behaviour.

Originality/value
There is a lot of ground that needs to be made in terms of designing programs capable of achieving impact in the Global South. The approach reported in this paper can serve as a best-practice model for researchers wanting to drive lasting behaviour change to overcome known inequities in the Global South.
Examining the relationships between instructional leadership, teacher self-efficacy and job satisfaction: a study of primary schools in India
Authors: Khan Furkan , Preeti  , Gupta Vishal 
Published: January 2024

PurposeBuilding on the social cognitive theory, a mediation model was examined to understand the role of teacher self-efficacy as the underlying mechanism for the relationship between instructional leadership and teacher job satisfaction.Design/methodology/approachThe study tests a mediation model between instructional leadership, teacher self-efficacy and job satisfaction. The data were collected via online survey from primary school teachers (N = 320) working for the Municipal Corporation of Delhi (MDC) in India. The mediation model was tested using the AMOS 22.0 after establishing the reliability and validity of measures.FindingsRegression analyses using the bootstrapping method indicated that teacher self-efficacy mediates the relationship between instructional leadership and teacher job satisfaction.Research limitations/implicationsThis is a cross-sectional study. The scope for causal inferences is, thus, limited.Practical implicationsIn the Indian setting, the study examines the association between instructional leadership and job satisfaction. The results show that the instructional leadership of the school principal is strongly related to teachers' self-efficacy, which, in turn is positively associated with teacher’s job satisfaction. Further, the findings confirm that instructional leadership, emphasizing instructional improvement, improves teachers' self-efficacy and job satisfaction.Originality/valueThe study explains the underlying process through which a school principal’s instructional leadership is related to teacher job satisfaction. This study is perhaps the first to focus on an Indian or a non-Western context.
The added value of environmental certification in the Dutch office market
Authors: van Overbeek Rens , Ishaak Farley , Geurts Ellen , Remøy Hilde 
Published: January 2024

Purpose This study examines the relationship between environmental building certification Building Research Establishment Environmental Assessment Method (BREEAM-NL) and office rents in the Dutch office market.Design/methodology/approach A hedonic price model was used to assess the impact of BREEAM certification on office rents. The study is based on 4,355 rent transactions in the period 2015 to mid-2022, in which 331 transactions took place in certified office buildings and 4,024 transactions in non-certified office buildings.Findings The results provide empirical evidence on quantitative economic benefits of BREEAM-certified offices in the Netherlands. After controlling for all important office rent determinants, the results show a rental premium for certified office buildings of 10.3% on average. The green premiums highly differ across submarkets and vary between 5.1 and 12.6% in the five largest Dutch cities. Additionally, the results show significant positive correlation between BREEAM-NL label score and rents, whereby better performing buildings generally command higher rents.Originality/value The study contributes to the current literature on green building economics by providing, as one of the first, empirical evidence on the existence of financial benefits for BREEAM-certified office buildings in the Dutch office market.
“The modesty guard” reflected in online comments in mainstream news sites
Authors: Druker Shitrit Shirley , Ben-Asher Smadar , Ben-Atar Ella 
Published: January 2024

Purpose
At times, a traditional minority group that opposes a change in the patriarchal structure is violent toward women who wish to adopt modern lifestyles. This study aims to examine online comments regarding a shooting at a café in an Arab-Bedouin city in Israel, where women were employed as servers. The event was framed in Israeli media as an act of backlash by young men, who call themselves “The Modesty Guard.”

Design/methodology/approach
In this qualitative study, the authors collected 916 online comments that were published on five main online news sites. A thematic and rhetorical analysis of online comments was conducted.

Findings
The findings uncovered five main themes: the expression of support for Bedouin women; ideas for dealing with the Modesty Guard; blaming Bedouin tradition for the shooting; comparison of the violent behavior to a parallel phenomenon among Charedim; and criticism of the lack of treatment by Israeli security forces. The responses reflected a supportive stance toward Arab-Bedouin women, who were open to progress. Conflict discourse, however, expressed alienation and increased social-national schism between the Jewish majority and Arab-Bedouin minority groups in Israel.

Originality/value
This study sheds light on the backlash phenomenon in Negev Bedouin society. Moreover, it exposes the lack of significant supportive actions and a lack of understanding of the deep processes unfolding in this traditional society.
Is South Asia the missing bloc in world trade? An analysis of South Asian FTAs’ compatibility with WTO rules
Authors: de Silva Edirimuni Nadeesh Rangana 
Published: January 2024

Purpose
South Asia is a region urgently seeking development, although it has failed in regional integration. It is the second least integrated region regarding the number of Free Trade Agreements (FTAs) and can thus be recognised as a missing bloc in the global multilateral system. This study aims to focus on South Asian FTAs and explores the problems of the inter-relations and compatibility between the systemic and regional trade systems.

Design/methodology/approach
The study proposes a framework to benchmark the compatibility of South Asian FTAs with WTO rules. Primary data from 2000 to 2020, including descriptive analyses of reports, legal text of the FTAs, official documents and factual presentations, have been collected and analysed through thematic analysis using the proposed framework.

Findings
The study finds that, although South Asian FTAs meet most of the WTO requirements, they are not progressing toward facilitating and promoting trade. Data from 2000 to 2020 show us that South Asian FTAs have not significantly impacted trade between themselves. The study argues that, although South Asian FTAs fulfil some benchmarks, they show only a lukewarm interest in contributing to the international trading system as building blocs. It is therefore recommended that the case of South Asian trade liberalisation must be understood contextually and be given careful and exclusive attention by the WTO.

Originality/value
As such, this study is the first to claim that South Asian FTAs are not fully compatible with the WTO rules. They remain a missing regional bloc in the multilateral system, rather than a building bloc or a stumbling bloc, delaying the region’s opportunity to develop as a region and within the larger system.