bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and organizational features of ulcerative colitis and Crohn's disease differential diagnosis: the experience of the specialized City clinic for Inflammatory Bowel Diseases
Authors: Uspenskiy Yu. P. , Ivanov S. V. , Fominykh Yu. A. , Lykova E. A. , Panasyuk A. S. , Dikarev K. V. , Gakhramanova A. A. 
Published: January 2025

Introduction. The initial diagnosis of ulcerative colitis and Crohn's disease – inflammatory bowel diseases (IBD) – is quite difficult, requiring a comprehensive evaluation of clinical and anamnesis data, the results of ilecolonoscopy with multifocal biopsy and, results of imaging diagnostic methods (in some cases).The aim of the study: to estimate the duration of the time period required to establish the IBD diagnosis of and to assess the potential consequences of untimely establishment of this diagnosis.Materials and methods. The data of 253 patients with ulcerative colitis and 263 patients with Crohn's disease who applied to the city center of St. Petersburg.Results. In 59 % of ulcerative colitis patients with and in 65 % Crohn's disease patients the diagnosis was established within 3 months from first clinical sighs appearance. Evaluation of the possible association between the duration of the period required to establish the diagnosis of IBD and features of the course of IBD after diagnosis did not reveal a statistically significant association with the rate of recurrence and the risk of developing non-life-threatening complications. When studying the development of life-threatening complications of IBD it was found that only 59 % of such complications occurred after the diagnosis of IBD, while in the remaining cases they occurred either before or caused the diagnosis. Untimely diagnosis of IBD increased the risk of life-threatening complications by 18 times in patients with ulcerative colitis, and by 13 times in patients with Crohn's disease, compared to the situation when the diagnosis was established timely and specific IBD therapy for was initiated. Conclusion. The majority of IBD patients, who suffered a life-threatening complication before the diagnosis was established, potentially had a temporary «window of opportunity», when on the basis of clinical data it was possible to suspect the presence of IBD, establish the diagnosis with the subsequent timely initiation of specific therapy to prevent a complicated course of the disease.
Comprehensive safety and toxicity analysis of 2,2’-Bipyridine derivatives in combating MRSA biofilm formation and persistence
Authors: Priyanka  , Sharma Mohini , Vaid Bhavna , Bharti Ram , Raut Sachin , Jolly R. S. , Khatri Neeraj 
Published: January 2025

IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections have become arduous to treat due to their capacity to form biofilms, develop persistence, and exhibit significant antimicrobial resistance. These factors contribute to the complexity of managing MRSA infections and highlight the urgent need for innovative treatment strategies.ObjectivesThis endeavor aims to evaluate the safety of 2,2’-Bipyridine (2,2’-Bipy) derivatives and their antimicrobial, anti-biofilm, and anti-persister activities in treating MRSA Infections.MethodsSix derivatives were screened for their ADMET properties and tested for minimum inhibitory concentrations against various bacterial strains using agar well diffusion and broth dilution. Safety studies were conducted through hemolysis tests, cell viability assays, and in vivo acute oral toxicity examinations. Bactericidal mechanisms and biofilm disruption effects were analyzed using crystal violet staining and confocal microscopy assays. The murine thigh infection model was also used to investigate the in vivo efficacy.ResultsAll derivatives exhibited favorable physicochemical profiles and ADMET properties and are predicted to be safe based on their drug-like properties. in vitro studies demonstrated that derivatives are non-toxic to 3T3 L1, and in vivo studies confirmed their safety in mice at a dose of 300 mg/kg and their non-hemolytic nature against rabbit red blood cells. All compounds showed potent antibacterial activity against the tested bacteria, including the resistant MRSA strain 831. They inhibited biofilm formation and eradicated biofilms in a dose-dependent manner against MTCC 737 and MRSA 831, and they effectively eliminated MRSA persister cells, outperforming the reference antibiotic vancomycin. These derivatives were found to depolarize the mitochondrial membrane and accumulate intracellular reactive oxygen species. These derivatives significantly reduced the bacterial load in the murine thigh infection model.ConclusionThe study concluded that 2,2’-Bipy derivatives possess significant antimicrobial activity, are non-toxic, and are effective in inhibiting biofilm formation and killing persister cells.
The impact of type 2 diabetes on left ventricular function in hypertensive patients: a three-dimensional speckle-tracking imaging study
Authors: Qingmei Yang , Xiaoyan Chen , Jianxiu Fang 
Published: January 2025

ObjectivesThe study aimed to evaluate the impact of the co-occurrence of hypertension and type 2 diabetes mellitus (T2DM) on the deterioration of left ventricular (LV) structure and function using three-dimensional speckle-tracking imaging (3D-STI), compared to patients with only hypertension.MethodsData from 272 hypertensive patients, including 85 with T2DM and 187 without, along with 45 normal controls, were analyzed. Participant characteristics were assessed before and after propensity score matching (PSM). 3D-STI-derived parameters, including LV function and global strain parameters, were compared among controls and different patient groups. Multivariable linear regression analyses were conducted to determine the impacts of T2DM on LV function and global strain. Additionally, linear mixed-effects regression models were used to evaluate the associations between 3D-STI-derived parameters and T2DM over time in hypertensive patients.ResultsSignificant increases in the E/e' ratio and declines in the LV global radial strain (GRS) were observed across the control group, HTN (T2DM-) group, and HTN (T2DM+) group. After adjusting for various factors using PSM analysis, LV global circumferential strain (GCS) and global area strain (GAS) were also found to be significantly decreased in the HTN (T2DM+) group compared to the HTN (T2DM-) group. Multivariable regression analyses, accounting for various covariates, indicated that T2DM was independently linked to LV strains (LV GAS: β = 0.95, 95% CI: 0.90–1.00, p = 0.029; LV GRS: β = 1.03, 95% CI: 1.01–1.06, p = 0.014) in hypertensive patients. Furthermore, linear mixed-model analysis revealed that LV GCS (β = 1.20, 95% CI: 0.38–2.01, p = 0.004) and GRS (β = −2.82, 95% CI: −4.97–0.68, p = 0.010) deteriorated over the 12-month period.ConclusionsT2DM exacerbates the decline in LV global and regional strains in patients with hypertension, and 3D-STI may be a valuable tool for detecting these asymptomatic preclinical abnormalities.
Video games and metacognition in the classroom for the development of 21st century skills: a systematic review
Authors: Checa-Romero Mirian , Gimenez-Lozano José Miguel 
Published: January 2025

IntroductionGame-based learning is a methodology that has gained importance in the world of education due to the benefits of implementing ICT in the classroom. Due to the intellectual and competence demands that some commercial video games promote, they can be very valuable tools to stimulate and promote metacognitive abilities in students. Therefore, this paper aimed to analyze the use of commercial video games in educational contexts and their impact on the development of metacognitive skills.MethodsFor the analysis of the study, a systematic review of the literature was carried out following the PRISMA statement. Specialized databases such as Scopus, WoS, PsyInfo, PubMed, SciELO, and Eric were consulted, which helped capture the essence of the problem between 2004 and 2024.ResultsThe review was then carried out on 10 selected articles. The results obtained show the following evidence: (a) commercial video games, together with the use of metacognitive strategies, enrich academic and social aspects as well as the metacognitive level, and (b) commercial video games are as effective in promoting metacognition as other more traditional methodologies or tools.DiscussionFinally, there is a need to rethink the implications of the use of video games in the classroom if we want students to acquire skills that will be essential to successfully respond to the demands and challenges of their academic and professional future.
Clinical significance of hyperuricaemia in biopsy-proven diabetic kidney disease ━ a single-centre retrospective study
Authors: Yu Jin , Tu Xiao , Xu Kunyue , Tang Xuanli , Wu Yufan , Jiang Xue 
Published: January 2025

AimsHyperuricaemia is associated with the development of Diabetic kidney disease (DKD). However, the mechanism of hyperuricaemia causing the progression of DKD remain unclear.MethodsThis is a single-centre retrospective study. 155 biopsy-proven DKD patients were grouped into hyperuricaemia and non-hyperuricaemia groups. Kaplan-Meier analysis and landmark curves were performed to explore predictors of end-stage renal disease (ESRD), Cox regression analysis was used to screen for factors, a nomogram was constructed to predict the renal prognosis of DKD.ResultsPatients in hyperuricaemia group had higher serum creatinine (Scr), degree of mesangial expansion and IFTA score and lower GFR, haemoglobin. SUA level was positively correlated with IFTA scores. The Kaplan-Meier curve and landmark analysis revealed worse survival in hyperuricaemia group, especially after 12 months. 11 variables, including age, sex, haemoglobin, Scr, SUA, and pathological score were collected to make a nomogram model. In the testing and training sets, the AUCs at 1, 3, and 5 years were 0.888, 0.939, and 0.886 and 0.947, 0.867, and 0.905, respectively.ConclusionThe clinicopathologic manifestation of DKD patients with hyperuricaemia was much more severe, and hyperuricaemia predicted a worse renal prognosis. A new renal prognosis prediction model including SUA was constructed for DKD with higher accuracy.
Case report: A panorama gene profile of ovarian cancer metastasized to axillary lymph node
Authors: Xia Yu , Huang Yu , Liu Zheng , Song Siyuan , Wang Yi , Luo Jing 
Published: January 2025

BackgroundOvarian cancer is among the most lethal gynecologic malignancies, with a high proportion of patients diagnosed at advanced stages, leading to poor survival outcomes. Axillary lymph node metastasis from ovarian cancer is extremely rare and the mechanism is still unclear.MethodsA comprehensive set of clinical and gynecologic oncology assessments were performed, including ultrasound, mammography, MRI, transvaginal ultrasound, and tissue staining. To unravel the carcinogenesis, the next-generation sequencing (NGS) was performed.ResultsConventional examinations and imaging suggested the presence of both occult breast cancer and ovarian cancer. However, immunohistochemical staining confirmed the diagnosis of high-grade serous ovarian carcinoma. Further analysis of NGS identified two novel missense mutations, D326E in BTK (Bruton’s tyrosine kinase) at SH2 domain and D251E in EPHA5 (EPH receptor A5), along with other known cancer- associated mutations. These mutations, particularly the novel missense mutations, may lead to metastasis to the axillary lymph nodes and drug resistance. Therefore, based on these findings, the chemotherapy regimen was adjusted accordingly.ConclusionThis is the first report on the panorama gene profile of ovarian cancer metastasis to axillary lymph node and we found two novel mutations (BTK pD326E and EPHA5 pD251E). This study unraveled the potential mechanism of genetic mutation for tumor metabolism, drug resistance, and metastasis.
Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages
Authors: Chen I-Hao , Georgopoulou Dimitra G. , Ebbesson Lars O. E. , Voskakis Dimitris , Munthe-Kaas Antonella Zanna , Papandroulakis Nikos 
Published: January 2025

IntoductionWith the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site.MethodsIn our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass (Dicentrarchus labrax) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns.ResultsOur results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB).DiscussionWe anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies.
Association between intrahepatic cccDNA and the severity of liver inflammation in chronic hepatitis B virus infection patients
Authors: Zhao Suxian , Dong Chen , Chang Chudi , Zhang Jiaming , Li Jing , Zhang Xiaoxiao , Ren Weiguang , Zhang Ying , Nan Yuemin 
Published: January 2025

Background and aimsThis research aimed to examine the association between hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and liver inflammation in chronic hepatitis B (CHB) infection patients.MethodsFrom August 2013 to June 2022, CHB patients at Hebei Medical University Third Hospital (Hebei, China) were recruited. Intrahepatic cccDNA was quantified and its association with liver inflammation was analyzed. Liver inflammation was assessed using the Ishak-modified histological activity index (HAI). Biochemical and viral indicators as well as hepatic inflammation biomarkers were monitored.ResultsIn total, 55 CHB patients were enrolled. The average HBV-cccDNA level was markedly elevated in HBeAg+ patients compared to HBeAg patients. Intrahepatic cccDNA levels differed significantly in different liver inflammation groups and showed a positive correlation with the HAI score for hepatic inflammation.ConclusionHBV-cccDNA level was associated with liver inflammation.
Infusion line contamination in preterm neonates: impact of infusion line design, length, and use duration: the multicenter ChronoBIOline study
Authors: Dos Santos Sandra , Valentin Anne-Sophie , Farizon Mathilde , Charbonneau Manon , Boukhris Mohamed Riadh , Brat Roselyne , Cazzorla Fabiana , Chauvel Jennifer , Cneude Fabrice , Coutable Pauline , Demasure Maryvonne , Duminil Emeline , Faraut-Derouin Vénonique , Muselli Maud Gits , Gorin Valérie , Goujon Rosemary , Guillouche-Puissant Melinda , Hacinlioglu Nadine , Landelle Caroline , Lefebvre Annick , Leroy-Terquem Elise , Martinet Aurore , Massebeuf Camille , Orfanos Nadia Mazille , Menard Guillaume , Menvielle Laure , Monin Vanessa , Morange Virginie , Patkai Juliana , Perrault Nathalie , Prat Emilie , van der Mee-Marquet Nathalie 
Published: January 2025

IntroductionCentral venous catheters are critical in preterm neonatal care but increase the risk of central line-associated bloodstream infections (CLABSIs). The incidence of S. haemolyticus-associated CLABSIs in French neonates is increasing, but the mechanisms underlying this trend remain unclear.MethodsWe examined microorganisms in 108 central line infusion sets used in preterm infants across 12 neonatal intensive care units, and collected at the time of removal.ResultsThe infusion sets varied widely in type (28 types; 1-6 parts) and length (10-180 cm, mean 52.9 cm). Contamination was detected in 24 infusion sets (22.2%), mainly by coagulase-negative Staphylococci (50.0%) and Bacillus species (41.7%). Higher contamination rates were linked to longer infusion lines (&gt; 50 cm; p &lt; 0.001), usage beyond 7 days (p = 0.002), and multi-line infusion systems (p &lt; 0.001).DiscussionOur findings are fully consistent with guidelines, which recommend simpler designs and a 4 or 7-day use of infusion sets, emphasizing the importance of adhering to these guidelines to reduce the risk of CLABSIs. Additionally, our findings raise concerns regarding the use of multi-line infusion systems. These devices, which combine extended infusion line length, manufacturer-authorized use of up to 21 days, and intermittent use of certain infusion lines, are easily contaminated during use, creating a high-risk situation for central line contamination.
Translingual neural stimulation induced changes in intra- and inter-network functional connectivity in mild-moderate traumatic brain injury patients
Authors: Chu Daniel Y. , Hou Jiancheng , Hosseini Thomas , Nair Veena A. , Adluru Nagesh , Danilov Yuri , Kaczmarek Kurt A. , Meyerand Mary E. , Tyler Mitchell , Prabhakaran Vivek 
Published: January 2025

IntroductionMild-to-moderate traumatic brain injury (mmTBI) that lead to deficits in balance and gait are difficult to resolve through standard therapy protocols, and these deficits can severely impact a patient's quality of life. Recently, translingual neural stimulation (TLNS) has emerged as a potential therapy for mmTBI-related balance and gait deficits by inducing neuroplastic changes in the brain gray matter structure. However, it is still unclear how interactions within and between functional networks in brain are affected by TLNS. The current study aimed to extend our previous resting-state functional connectivity (RSFC) study investigating the effects of TLNS intervention on outcome measures related to gait and balance.MethodsAn experimental PoNS device was utilized to deliver the TLNS. The 2-week TLNS intervention program, specifically stimulation during focused physical therapy focused on recovery of gait and balance, included twice-daily treatment in the laboratory and the same program at home during the intervening weekend. The resting-state fMRI datasets at pre- and post-interventions were collected by 3T MRI scanner with nine mmTBI patients. All participants also received both Sensory Organization Test (SOT) and Dynamic Gait Index (DGI) testing pre- and post-intervention as part of the behavioral assessment.ResultsCompared to baseline, TLNS intervention led to statistically significant improvements in both the SOT [t(8) = 2.742, p = 0.028] and the DGI [t(8) = 2.855, p = 0.024] scores. Moreover, significant increases in intra- and inter-network RSFC were observed, particularly within the visual, default mode, dorsal attention, frontoparietal (FPN), and somatosensory (SMN) networks. Additionally, there were significant correlations between the SOT and inter-network FC [between FPN and SMN, r(9) = –0.784, p = 0.012] and between the DGI and intra-network FC [within SMN, r(9) = 0.728, p = 0.026].DiscussionThese findings suggest that TLNS intervention is an effective in increasing somatosensory processing, vestibular-visual interaction, executive control and flexible shifting, and TLNS may be an effective approach to inducing brain network plasticity and may serve as a potential therapy for mmTBI-related gait and balance deficits.
Efficacy of fish oil supplementation on metabolic dysfunction-associated steatotic liver disease: a meta-analysis
Authors: Zhou Like , Sun Dongmei , Bai Houqiao 
Published: January 2025

ObjectiveGlobally, the occurrence of Metabolic dysfunction-associated steatotic liver disease (MASLD) is on a steady rise. Fish oil has anti-inflammatory effects and can improve lipid metabolism. The article aims to assess the impact of fish oil supplementation on MASLD.MethodsWe conducted a systematic search of Cochrane, Embase, PubMed, and Web of Science up to September 31, 2024, for randomized control trials (RCTs). The risk of bias of the included RCTs was evaluated using the Cochrane Collaboration’s tool. Outcomes measured were aspects of liver injury, lipid profile, insulin resistance, anthropometric measurements, and more.ResultsSeven randomized controlled trials (RCTs) involving 439 participants were incorporated into the analysis. In general, the risk of bias in these RCTs was either low or not clearly defined. Pooled analysis showed that triglycerides [TG, pooled standard mean difference (SMD): −0.40 (95% CI: −0.58 to −0.21)], aspartate transaminase [AST, SMD: −0.29 (95% CI: −0.48 to −0.10)], HOMA-IR [SMD: −2.06 (95% CI: −3.36 to −0.49)] and waist circumference [Waist-C, SMD: −0.31 (95% CI: −0.54 to −0.08)] were significantly improved. But showed no significant benefits on alanine transaminase [ALT, SMD: −0.15 (95% CI: −0.45 to 0.15)], gamma-glutamyl transpeptidase [GGT, SMD: −0.07 (95% CI: −0.26 to 0.12)], body mass index [BMI, SMD: 0.16 (95% CI: −0.34 to 0.02)], high-density lipoprotein cholesterol [HDL, SMD: 0.02 (95% CI: −0.18 to 0.22)], low-density lipoprotein cholesterol [LDL, SMD: −0.01 (95% CI: −0.20 to 0.18)], Total Cholesterol [TC, SMD: −0.34 (95% CI: −0.70 to 0.01)] and so on.ConclusionThe current evidence supports the fish oil supplementation in improving MASLD. Fish oil supplementation may also regulate blood lipids and improve glucose metabolism disorders.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#myprospero, identifier CRD42024513246.
Cresp®: transforming the landscape of chemotherapy-induced anemia - a comprehensive retrospective real-world analysis in 523 Indian patients
Authors: Chandrakanth MV , Agarwala Vivek , Sopory Pranav , Nayak Himadri , Parikh Purvish M. , Roy Minakshi , De Rajib , Narayan Pradeep , Barai Anjuli Tuladhar , Mandal Kaustav , Basu Moinak , Kumar Subhabrata , Uppal Rajdeep Singh , Naqvi Syed Mujtaba Hussain , Desai Rohit 
Published: January 2025

IntroductionAnemia, a frequently encountered issue among cancer patients undergoing chemotherapy, is regrettably underappreciated despite its prevalence and profound impact on their well-being. Chemotherapy-induced anemia (CIA) diminishes the quality of life, causing fatigue, breathlessness, and a decline in the performance status. However, correcting anemia can lead to notable improvements in these parameters. Notably, darbepoetin alpha (DA) has shown efficacy in addressing anemia in this context. This real-world study aims to evaluate the efficacy of DA in the treatment of CIA among Indian cancer patients.MethodsThis single-center retrospective study assessed the effectiveness of DA in treating CIA among advanced/metastatic solid tumor patients on palliative myelosuppressive therapy. The study measured the change in hemoglobin levels after DA administration as the primary outcome, with secondary outcomes including impact on blood transfusion dependence, changes in anemia-related symptoms, and occurrence of adverse events.ResultsA total of 523 patients, with a median age of 55, were included in the study. Patients were categorized based on cancer site, type of chemotherapy, response to therapy, and DA doses. A significant mean increase of 2.28 gm/dl in hemoglobin (Hb) levels from baseline to post-DA administration was observed (8.56±0.45 to 10.84±0.92; 26.6%; P&lt;.001). Each sub-group revealed a significant enhancement of mean hemoglobin from baseline to the end of treatment. Significant improvements were noted from baseline in fatigue, and dyspnea. Adverse drug reactions included hypertension (5.4%), deep vein thrombosis (2.9%), and arrhythmias (0.8%).DiscussionDA demonstrates impressive efficacy and safety in managing CIA, leading to substantial improvements in mean hemoglobin levels in palliative setting. This has the potential to reduce the need for blood transfusions and enhance the quality of life for patients.