bluuebunny/crossref_metadata_2025_split huggingface dataset:
All‐Optical Thermometry Monitoring Biochemical Kinetics with NV Centers in Diamond
Authors: Li Min , Zhang Qi , Kong Xi , Li Zhijie , Yang Zhiping , Zhao Sheng , Qin Zhuoyang , Kong Fei , Chen Sanyou , Su Jia , Ruan Ke , Shi Fazhan , Du Jiangfeng 
Published: January 2024

AbstractNanothermometers enable label‐free measurement of biochemical processes with extremely small sample consumption, which is essential to high‐throughput assays for enzyme searching and drug screening. However, high sensitivity and long‐term chemical stability are still crucial challenges for current nanothermometers. Here, the nitrogen‐vacancy centers in diamond are chosen as atomic thermometers and apply the diamond thermometry to kinetic monitoring of biochemical reactions. The performance of all‐optical diamond thermometry are tested under severe chemical conditions with strong acids and bases. Diamond thermometry demonstrates a sensitivity of 80 mK and chemical stability of 62 mK even in the presence of a drastic pH change during neutralization reactions. The thermodynamics of two enzyme‐catalyzed processes: adenosine triphosphate hydrolysis and urea hydrolysis, are further analyzed. The enthalpy changes obtained at the submicron scale by diamond thermometry are in good agreement with commercial macroscopic isothermal titration calorimetry. This method could offer a universal characterization technique for biochemical studies at the femtoliter scale.
Parental involvement in shared book reading for preschoolers in China: Patterns and risks
Authors: Tian Xingjiang , Cui Shujing , Greger David 
Published: January 2024

AbstractPrevious studies have elucidated the positive effect of the quantity and onset age of shared book reading on children's language development. Few studies have addressed the profiles of parental involvement in terms of different key aspects of shared book reading. This study adopted a latent profile analysis to examine the patterns of parental involvement in shared book reading and associated factors; 980 Chinese parents of kindergarten children were investigated. Three profiles were identified: (1) late start and infrequent reading; (2) early start and frequent reading; (3) medium start and intensive reading. Higher socioeconomic status, more literacy resources, and older age of the children were all found to be more likely to be associated with identification with Profile 2 than Profile 1. Implications for more targeted parental intervention programmes to broaden access to home literacy resources are highlighted.
Efficient off‐policy Q‐learning for multi‐agent systems by solving dual games
Authors: Wang Yan , Xue Huiwen , Wen Jiwei , Liu Jinfeng , Luan Xiaoli 
Published: January 2024

AbstractThis article develops distributed optimal control policies via Q‐learning for multi‐agent systems (MASs) by solving dual games. According to game theory, first, the distributed consensus problem is formulated as a multi‐player non‐zero‐sum game, where each agent is viewed as a player focusing only on its local performance and the whole MAS achieves Nash equilibrium. Second, for each agent, the anti‐disturbance problem is formulated as a two‐player zero‐sum game, in which the control input and external disturbance are a pair of opponents. Specifically, (1) an offline data‐driven off‐policy for distributed tracking algorithm based on momentum policy gradient (MPG) is developed, which can effectively achieve consensus of MASs with guaranteed ‐bounded synchronization error. (2) An actor‐critic‐disturbance neural network is employed to implement the MPG algorithm and obtain optimal policies. Finally, numerical and practical simulation results are conducted to verify the effectiveness of the developed tracking policies via MPG algorithm.
Nonsingular predefined‐time dynamic surface control of a flexible‐joint space robot with actuator constraints
Authors: Liu Liaoxue , Gu Xiutao , Wang Lu , Guo Jian , Guo Yu 
Published: January 2024

AbstractTo achieve predefined‐time trajectory tracking control of a flexible‐joint space robot(FJSR) with actuator constraints, a nonsingular predefined‐time dynamic surface control scheme is developed. The input saturation caused by actuator constraints is addressed via the designed predefined‐time anti‐saturation compensator. On this basis, two different control laws are designed for such high‐order nonlinear systems by utilizing the backstepping technique, and a novel nonlinear filter is constructed to filter the virtual control signals, thus avoiding the “differential expansion” phenomenon. Moreover, a singularity‐free auxiliary function is designed to solve the singularity issue generated by the derivative of fractional power terms in the predefined‐time control algorithm framework. The closed‐loop system is proven to be semi‐globally predefined‐timely uniformly ultimately bounded (SGPTUUB) via constructing the suitable Lyapunov function. The difference and effectiveness of the two designed control laws are illustrated by the conducted simulations. Both of them allow the FJSR system to track the desired trajectory in a reasonably predefined time.
Mapping the literature on decent work: A bibliometric analysis of sustainable development goal 8
Authors: Ralph Alisha , Arora Akarsh 
Published: January 2024

AbstractThe bibliometric study based on the Scopus database aims to investigate the global literary development of “decent work” over a span exceeding two decades. The noticeable surge in publications since 2015 underscores the growing significance of the concept. Limited representation of lower‐middle and lower‐income countries, except India, among the top 10 contributors is observed, revealing a considerable research gap in the study of decent work. The research highlights five principal themes: the labor market's impact on economic development, corporate social responsibility and sustainable development, gender equality, governance, and social justice, as well as decent work, job quality, education, well‐being, and job satisfaction. The study also shows the importance of the topic in different fields. Future researchers may consider the variables or indicators identified for surveys and questionnaire construction.
Media framing of sustainability: The case of British pharmaceutical companies (2000–2020)
Authors: Zec Dejan N. 
Published: January 2024

AbstractCorporate sustainability is a complex concept, creating ambiguity regarding what it means and entails in a given context. Especially in sectors such as the pharmaceutical industry, where there is little direct interaction between the company and its stakeholders, infomediaries such as the mass media play a pivotal role in framing corporate sustainability and providing social evaluations to the general public. This paper reports on a longitudinal frame analysis of the newspaper reporting on corporate sustainability related to two British pharmaceutical companies, AstraZeneca and GlaxoSmithKline, in the period between 2000 and 2020. It explores and explains dominant sustainability frames related to pharmaceutical industry. The findings suggest that newspaper frames prioritize social and economic sustainability dimensions, related to companies' core business activities and relationships with stakeholders, and neglect the environmental dimension. The paper contributes to the existing literature by exploring contextual media framing, sustainability complexity and, empirically, sustainability in the pharmaceutical industry.
Long‐term survival of a cat diagnosed with primary hepatic neuroendocrine carcinoma treated with surgical resection and several adjuvant chemotherapies
Authors: Ruffin Christine J. , Burge Rhonda , Jennings Ryan N. , Hostnik Eric T. , Selmic Laura E. 
Published: January 2024

AbstractPrimary hepatic neuroendocrine carcinoma, previously referred to as carcinoid, is a malignant tumour arising from neuroendocrine cells and is a rare form of hepatic neoplasia in cats. This case report describes primary hepatic neuroendocrine carcinoma in a cat, with weight loss and vomiting as its primary clinical signs. Definitive diagnosis was made following surgical excision and histopathology of the affected liver lobes in addition to immunohistochemistry staining. Following surgery, the cat received different chemotherapy agents due to observation of progressive disease at restaging visits. The cat is currently receiving metronomic chemotherapy of cyclophosphamide and has no evidence of metastatic disease present 1009 days after initial presentation. Primary hepatic neuroendocrine carcinoma can occur in cats and should be a differential diagnosis for a hepatic mass in this species. Primary hepatic neuroendocrine carcinomas are rare in cats, and there is no standard of care for treatment in companion animals.
Verification of nucleotide sequence reagent identities in original publications in high impact factor cancer research journals
Authors: Pathmendra Pranujan , Park Yasunori , Enguita Francisco J. , Byrne Jennifer A. 
Published: January 2024

AbstractHuman gene research studies that describe wrongly identified nucleotide sequence reagents have been mostly identified in journals of low to moderate impact factor, where unreliable findings could be considered to have limited influence on future research. This study examined whether papers describing wrongly identified nucleotide sequences are also published in high-impact-factor cancer research journals. We manually verified nucleotide sequence identities in original Molecular Cancer articles published in 2014, 2016, 2018, and 2020, including nucleotide sequence reagents that were claimed to target circRNAs. Using keywords identified in some 2018 and 2020 Molecular Cancer papers, we also verified nucleotide sequence identities in 2020 Oncogene papers that studied miRNA(s) and/or circRNA(s). Overall, 3.8% (251/6647) and 4.0% (47/1165) nucleotide sequences that were verified in Molecular Cancer and Oncogene papers, respectively, were found to be wrongly identified. Wrongly identified nucleotide sequences were distributed across 18% (91/500) original Molecular Cancer papers, including 38% (31/82) Molecular Cancer papers from 2020, and 40% (21/52) selected Oncogene papers from 2020. Original papers with wrongly identified nucleotide sequences were therefore unexpectedly frequent in two high-impact-factor cancer research journals, highlighting the risks of employing journal impact factors or citations as proxies for research quality.
High risk of early recurrent stroke in patients with near-occlusion with full collapse of the internal carotid artery
Authors: Henze Alexander , Fox Allan J. , Johansson Elias 
Published: January 2024

AbstractWe aimed to validate the prognostic ability and assess interrater reliability of a recently suggested measurement-based definition of near-occlusion with full collapse (distal ICA diameter ≤ 2.0 mm and/or ICA ratio ≤ 0.42). 118 consecutive patients with symptomatic near-occlusion were prospectively included and assessed on computed tomography angiography by 2 blinded observers, 26 (22%) had full collapse. At 2 days after presenting event, the risk of preoperative stroke was 3% for without full collapse and 16% for with full collapse (p = 0.01). At 28 days, this risk was 16% for without full collapse and 22% for with full collapse (p = 0.22). Interrater reliability was perfect (kappa 1.0). Thus, near-occlusion with full collapse should be defined as distal ICA ≤ 2.0 mm and/or ICA ratio ≤ 0.42 in order to detect cases with very high risk of early stroke recurrence.
Sharp-Interface Limit of a Multi-phase Spectral Shape Optimization Problem for Elastic Structures
Authors: Garcke Harald , Hüttl Paul , Kahle Christian , Knopf Patrik 
Published: January 2024

AbstractWe consider an optimization problem for the eigenvalues of a multi-material elastic structure that was previously introduced by Garcke et al. (Adv. Nonlinear Anal. 11:159–197, 2022). There, the elastic structure is represented by a vector-valued phase-field variable, and a corresponding optimality system consisting of a state equation and a gradient inequality was derived. In the present paper, we pass to the sharp-interface limit in this optimality system by the technique of formally matched asymptotics. Therefore, we derive suitable Lagrange multipliers to formulate the gradient inequality as a pointwise equality. Afterwards, we introduce inner and outer expansions, relate them by suitable matching conditions and formally pass to the sharp-interface limit by comparing the leading order terms in the state equation and in the gradient equality. Furthermore, the relation between these formally derived first-order conditions and results of Allaire and Jouve (Comput. Methods Appl. Mech. Eng. 194:3269–3290, 2005) obtained in the framework of classical shape calculus is discussed. Eventually, we provide numerical simulations for a variety of examples. In particular, we illustrate the sharp-interface limit and also consider a joint optimization problem of simultaneous compliance and eigenvalue optimization.
Expression of virus-like particles (VLPs) of foot-and-mouth disease virus (FMDV) using Saccharomyces cerevisiae
Authors: Le Ngoc My Tieu , So Kum-Kang , Chun Jeesun , Kim Dae-Hyuk 
Published: January 2024

Abstract
We engineered Saccharomyces cerevisiae to express structural proteins of foot-and-mouth disease virus (FMDV) and produce virus-like particles (VLPs). The gene, which encodes four structural capsid proteins (VP0 (VP4 and VP2), VP3, and VP1), followed by a translational “ribosomal skipping” sequence consisting of 2A and protease 3C, was codon-optimized and chemically synthesized. The cloned gene was used to transform S. cerevisiae 2805 strain. Western blot analysis revealed that the polyprotein consisting of VP0, VP3, and VP1 was processed into the discrete capsid proteins. Western blot analysis of 3C confirmed the presence of discrete 3C protein, suggesting that the 2A sequence functioned as a “ribosomal skipping” signal in the yeast for an internal re-initiation of 3C translation from a monocistronic transcript, thereby indicating polyprotein processing by the discrete 3C protease. Moreover, a band corresponding to only VP2, which was known to be non-enzymatically processed from VP0 to both VP4 and VP2 during viral assembly, further validated the assembly of processed capsid proteins into VLPs. Electron microscopy showed the presence of the characteristic icosahedral VLPs. Our results clearly demonstrate that S. cerevisiae processes the viral structural polyprotein using a viral 3C protease and the resulting viral capsid subunits are assembled into virion particles.

Key points
• Ribosomal skipping by self-cleaving FMDV peptide in S. cerevisiae.
• Proteolytic processing of a structural polyprotein from a monocistronic transcript.
• Assembly of the processed viral capsid proteins into a virus-like particle.
A novel conserved B-cell epitope in pB602L of African swine fever virus
Authors: Song Jinxing , Wang Mengxiang , Zhou Lei , Tian Panpan , Sun Junru , Sun Zhuoya , Guo Chenyun , Wu Yanan , Zhang Gaiping 
Published: January 2024

Abstract
African swine fever virus (ASFV) is a complex DNA virus and the only member of the Asfarviridae family. It causes high mortality and severe economic losses in pigs. The ASFV pB602L protein plays a key role in virus assembly and functions as a molecular chaperone of the major capsid protein p72. In addition, pB602L is an important target for the development of diagnostic tools for African swine fever (ASF) because it is a highly immunogenic antigen against ASFV. In this study, we expressed and purified ASFV pB602L and validated its immunogenicity in serum from naturally infected pigs with ASFV. Furthermore, we successfully generated an IgG2a κ subclass monoclonal antibody (mAb 7E7) against pB602L using hybridoma technology. Using western blot and immunofluorescence assays, mAb 7E7 specifically recognized the ASFV Pig/HLJ/2018/strain and eukaryotic recombinant ASFV pB602L protein in vitro. The 474SKENLTPDE482 epitope in the ASFV pB602L C-terminus was identified as the minimal linear epitope for mAb 7E7 binding, with dozens of truncated pB602l fragments characterized by western blot assay. We also showed that this antigenic epitope sequence has a high conservation and antigenic index. Our study contributes to improved vaccine and antiviral development and provides new insights into the serologic diagnosis of ASF.

Key points
• We developed a monoclonal antibody against ASFV pB602L, which can specifically recognize the ASFV Pig/HLJ/2018/ strain.
• This study found one novel conserved B-cell epitope474SKENLTPDE482.
• In the 3D structure,474SKENLTPDE482is exposed on the surface of ASFV pB602L, forming a curved linear structure.
Evaluation of selected tropical marine microalgal cultures for use in biophotovoltaic platforms
Authors: Tay Zoe Hui-Yee , Ng Fong-Lee , Thong Cheng-Han , Lee Choon-Weng , Gnana kumar G. , Al-Sehemi Abdullah G. , Phang Siew-Moi 
Published: January 2024

Abstract
In this study, the bioelectrical power generation potential of four tropical marine microalgal strains native to Malaysia was investigated using BPV platforms. Chlorella UMACC 258 produced the highest power density (0.108 mW m−2), followed by Halamphora subtropica UMACC 370 (0.090 mW m−2), Synechococcus UMACC 371 (0.065 mW m−2) and Parachlorella UMACC 245 (0.017 mW m−2). The chlorophyll-a (chl-a) content was examined to have a linear positive relationship with the power density (p < 0.05). The photosynthetic performance of strains was studied using the pulse-amplitude modulation (PAM) fluorometer; parameters measured include the following: maximum quantum efficiency (Fv/Fm), alpha (α), maximum relative electron transport rate (rETRmax), photo-adaptive index (Ek) and non-photochemical quenching (NPQ). The Fv/Fm values of all strains, except Synechococcus UMACC 371, ranged between 0.37 and 0.50 during exponential and stationary growth phases, suggesting their general health during those periods. The low Fv/Fm value of Synechococcus UMACC 371 was possibly caused by the presence of background fluorescence from phycobilisomes or phycobiliproteins. Electrochemical studies via cyclic voltammetry (CV) suggest the presence of electrochemically active proteins on the cellular surface of strains on the carbon anode of the BPV platform, while morphological studies via field emission scanning electron microscope (FESEM) imaging verify the biocompatibility of the biofilms on the carbon anode.

Key points
• Maximum power output of 0.108 mW m−2 is recorded by Chlorella UMACC 258
• There is a positive correlation between chl-a content and power output
• Proven biocompatibility between biofilms and carbon anode sans exogenous mediators
Synthesis and application of a phenazine class substrate for high-throughput screening of laccase activity
Authors: Babinskas Justinas , Sabotič Jerica , Matijošytė Inga 
Published: January 2024

AbstractBiocatalysis is one of the greatest tools for implementing the 12 principles of Green chemistry. Biocatalysts are bio-based, highly efficient and selective, operate at moderate conditions, and can be reused multiple times. However, the wider application of biocatalysts is plagued by a plethora of drawbacks, such as poor stability at operating conditions, inadequate efficiency of catalytic systems, a small number of commercially available biocatalysts, and a lack of substrates or methods for their discovery and development. In this work, we address the lack of suitable substrates for high-throughput screening of laccase by synthesising and investigating a newly developed phenazine-type substrate – Ferbamine. Investigation of Ferbamine pH and thermal stability indicated that its long-term stability in an aqueous medium is superior to that of commercially available substrates and does not require organic solvents. Ferbamine displayed convincing performance in detecting laccase activity on Ferbamine-agar plates in commercial laccase products and the collection of extracts from wild terrestrial fungi (42 species, 65 extracts), of which 26 species have not been described to have laccase activity prior to this work. Incubation of microorganisms on Ferbamine-agar plates showed its compatibility with live colonies. Ferbamine proved to be an easy-to-use substrate, which could be a great addition to the toolbox of methods for the functional analysis of laccases.
Impact on cardiovascular outcome of coronary revascularization-induced changes in ischemic perfusion defect and myocardial flow reserve
Authors: Assante Roberta , Zampella Emilia , D’Antonio Adriana , Mannarino Teresa , Gaudieri Valeria , Nappi Carmela , Arumugam Parthiban , Panico Mariarosaria , Buongiorno Pietro , Petretta Mario , Cuocolo Alberto , Acampa Wanda 
Published: January 2024

Abstract
Purpose
We evaluated the impact on cardiovascular outcome of coronary revascularization-induced changes in ischemic total perfusion defect (ITPD) and myocardial flow reserve (MFR) as assessed by 82Rb positron emission tomography (PET)/computed tomography (CT) imaging.

Methods
The study included 102 patients referred to 82Rb PET/CT myocardial perfusion imaging before and after coronary revascularization. All patients were followed for the occurrence of cardiovascular events (cardiac death, nonfatal myocardial infarction, repeated revascularization, and heart failure) after the second imaging study.

Results
During a median follow-up of 20 months, 21 events occurred. The clinical characteristics were comparable between patients with and without events. In the overall study population, after revascularization, there was a significant reduction (P < 0.001) of ITPD, while hyperemic myocardial blood flow (MBF) (P < 0.01) and MFR (P < 0.05) significantly improved. Event rate was higher in patients with ITPD (P < 0.005) or MFR (P < 0.001) worsening compared to those with unchanged or improved ITPD or MFR. At Cox univariable analysis, ITPD and MFR worsening resulted in predictors of events (both P < 0.05). Patients with worsening of both ITPD and MFR had the worst event-free survival (log-rank 32.9, P for trend < 0.001).

Conclusions
In patients with stable CAD, worsening of ITPD and MFR after revascularization procedures is associated with higher risk of cardiovascular events. Follow-up MPI with 82Rb PET/CT may improve risk stratification in patients submitted to coronary revascularization.
Psychosoziales Wohlbefinden und mögliche Wechselwirkungen mit Nacken- und Schulterbeschwerden
Authors: Jedlicka F. C. , Guelen E. , Fehrmann E. 
Published: January 2024

ZusammenfassungNacken- und Schulterbeschwerden gehören zu den am meisten verbreiteten Schmerzen in der modernen Gesellschaft. Neben allgemeinen Risikofaktoren wie weibliches Geschlecht, Länge der täglichen Computer‑/Handynutzung, mangelnde Körperaktivität und Vorkommen von ähnlichen Schmerzen in der Vergangenheit spielen psychosoziale sowie emotionale Risikofaktoren eine große Rolle. Maladaptive Einstellungen, Glaubenssätze und Kognitionen bezüglich der Erwartungen von Schmerzempfinden, Katastrophisierungstendenzen, niedrige Selbstwirksamkeitserwartungen sowie Angst-Vermeidungsverhalten können das Vorkommen und die Chronifizierung von Nacken- und Schulterschmerzen negativ beeinflussen. Inadäquater Umgang mit Stress im Alltag und im Arbeitskontext sowie mangelnder sozialer Rückhalt können sich zudem als körperliche Schmerzsymptomatik in der Nacken- und Schulterregion manifestieren. Emotionale Faktoren wie Angst und Depressionen weisen hierbei wechselseitige Beeinflussungen auf das Schmerzempfinden auf und wirken schmerzauslösend und -aufrechterhaltend. Psychologische Therapien sollten dabei helfen, psychoemotionale Resilienzfaktoren zu stärken sowie vorhandene stress- und schmerzbezogene maladaptive Bewältigungsstrategien und Glaubenssätze zu verändern. Insbesondere das Implementieren von kognitiv-verhaltenstherapeutischen und achtsamkeitsbasierten Therapieansätzen in ein biopsychosoziales Konzept zur Behandlung von Schulter- und Nackenschmerzen könnte eine gezielte Verbesserung des psychosozialen Wohlbefindens fördern.