bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stratification of the risk of ovarian dysfunction by studying the complexity of intermediate and premutation alleles of the FMR1 gene
Authors: Quilichini Juliette , Perol Sandrine , Cuisset Laurence , Grotto Sarah , Fouveaut Corinne , Barbot Jean Claude , Verebi Camille , Jordan Pénélope , Héron Delphine , Molina‐Gomes Denise , Pipiras Eva , Grynberg Michael , Catteau‐Jonard Sophie , Touraine Philippe , Christin‐Maître Sophie , Plu‐Bureau Geneviève , El Khattabi Laila , Bienvenu Thierry 
Published: November 2023

AbstractFMR1 premutation female carriers are at risk of developing premature/primary ovarian insufficiency (POI) with an incomplete penetrance. In this study, we determined the CGG repeat size among 1095 women with diminished ovarian reserve (DOR) / POI and characterized the CGG/AGG substructure in 44 women carrying an abnormal FMR1 repeat expansion number, compared to a group of 25 pregnant women carrying an abnormal FMR1 CGG repeat size. Allelic complexity scores of the FMR1 gene were calculated and compared between the two groups. In the DOR/POI cohort, 2.1% of women presented with an intermediate repeat size and 1.9% with a premutation. Our results suggest that the risk of POI is highest in the mid‐range of CGG repeats. We observed that the allelic score is significantly higher in POI women compared to the pregnant women group (p‐value = 0.02). We suggest that a high allelic score due to more than 2 AGG interspersions in the context of an intermediate number of repetitions could favor POI. Larger studies are still needed to evaluate the relevance of this new tool for the determination of the individual risk of developing POI in women with abnormal number of CGG repeats.
Exchangeable Self‐Assembled Lanthanide Antennas for PLIM Microscopy
Authors: Ruiz‐Arias Alvaro , Fueyo‐González Francisco , Izquierdo‐García Carolina , Navarro Amparo , Gutiérrez‐Rodríguez Marta , Herranz Rosario , Burgio Chiara , Reinoso Antonio , Cuerva Juan M. , Orte Angel , González‐Vera Juan A. 
Published: November 2023

AbstractLanthanides have unique photoluminescence (PL) emission properties, including very long PL lifetimes. This makes them ideal for biological imaging applications, especially using PL lifetime imaging microscopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for quantitative biological imaging. Unfortunately, due to the required prolonged acquisitions times, photobleaching of lanthanide PL emission currently constitutes one of the main drawbacks of PLIM. In this study, we report a small aqueous‐soluble, lanthanide antenna, 8‐methoxy‐2‐oxo‐1,2,4,5‐tetrahydrocyclopenta[de]quinoline‐3‐phosphonic acid, PAnt, specifically designed to dynamically interact with lanthanide ions, serving as exchangeable dye aimed at mitigating photobleaching in PLIM microscopy in cellulo. Thus, self‐assembled lanthanide complexes that may be photobleached during image acquisition are continuously replenished by intact lanthanide antennas from a large reservoir. Remarkably, our self‐assembled lanthanide complex clearly demonstrated a significant reduction of PL photobleaching when compared to well‐established lanthanide cryptates, used for bioimaging. This concept of exchangeable lanthanide antennas opens new possibilities for quantitative PLIM bioimaging.
RAFT Polymerisation by the Radical Decarboxylation of Carboxylic Acids**
Authors: Ayurini Meri , Haridas Darsan , Mendoza David Joram , Garnier Gil , Hooper Joel F. 
Published: November 2023

AbstractThe controlled grafting of polymers from small‐ and macro‐molecular substrates is an essential process for many advanced polymer applications. This usually requires the pre‐functionalisation of substrates with an appropriate functional group, such as a RAFT agent or ATRP initiator, which requires additional synthetic steps. In this paper, we describe the direct grafting of RAFT polymers from carboxylate containing small molecules and polymers via photochemical radical decarboxylation. This method utilises the innate functional groups present in the substrates, and achieves efficient polymer initiation in a single step with excellent control of molecular weight and dispersity.
Social motivation by self‐ and caregiver‐report: Reporter concordance and social correlates among autistic and neurotypical youth
Authors: Neuhaus Emily , Bernier Raphael A. , Webb Sara Jane 
Published: November 2023

AbstractDifferences in social motivation underlie the core social‐communication features of autism according to several theoretical models, with decreased social motivation among autistic youth relative to neurotypical peers. However, research on social motivation often relies on caregiver reports and rarely includes firsthand perspectives of children and adolescents with autism. Furthermore, social motivation is typically assumed to be constant across social settings when it may actually vary by social context. Among a sample of 58 verbally fluent youth (8–13 years old; 22 with autism, 36 neurotypical), we examined correspondence between youth and caregiver reports of social motivation with peers and with adults, as well as diagnostic group differences and associations with social outcomes. Results suggest youth and caregivers provide overlapping but distinct information. Autistic youth had lower levels of social motivation relative to neurotypical youth, and reported relatively consistent motivation toward peers and adults. Youth self‐ and caregiver‐report were correlated for motivation toward adults, but not toward peers. Despite low correspondence between self‐ and caregiver‐reported motivation toward peers, autistic youths' self‐report corresponded to caregiver‐reported social skills and difficulties whereas caregiver‐report of peer motivation did not. For neurotypical youth, self‐ and caregiver‐reported motivation toward adults was correlated, but motivation by both reporters was largely independent of broader social outcomes. Findings highlight the unique value of self‐report among autistic children and adolescents, and warrant additional work exploring the development, structure, and correlates of social motivation among autistic and neurotypical youth.
Pro‐catalytic Role of Transition Metal/Metal Oxide Blend Low Pd Catalysts for Ethanol Oxidation: Diminution of Activation Energy and Promoting Electrode Kinetics at Low Temperature
Authors: Mondal Achintya , Adhikary Rajib , Hazra Mukul , De Abhishek , Datta Jayati 
Published: November 2023

AbstractIn this investigation the effect of temperature on catalytic oxidation of ethanol on multi‐metallic PdCoNi/C nano‐composite bearing reduced palladium loading, have been studied over the temperature range 20 °C to 80 °C. The structure, composition and surface morphology of the catalyst regime were determined through the respective techniques like XRD, EDX and TEM while electro‐catalytic parameters were evaluated by the help of voltammetry, chronoamperometry, impedance spectroscopy and ion‐chromatography. The apparent activation energies (Ea) of ethanol oxidation reaction (EOR) were drastically reduced over PdCoNi/C compared to Pd/C reflecting the energy efficiency of ternary matrix furnished with only 35 % Pd loading and the rest 65 % being cheaper transition metals. The long term poisoning rates warrant improved stability of PdCoNi/C and also figure out the robustness of the catalyst even at 20 °C. The Ea value is remarkably reduced, rendering appreciable mass activity in terms of current density per mg of Pd at each of the temperatures studied. The incorporation of transition metals Co and Ni onto Pd becomes particularly effective in orienting the EOR proceed through carbonate pathway as reflected by the columbic efficiency in terms of oxidation product yield ~5 times higher, compared to single Pd catalyst throughout the temperature range.
Influence of fungicide selection and application timing on take‐all root rot management under field and greenhouse conditions
Authors: Stephens Cameron M. , Gannon Travis W. , Cubeta Marc A. , Kerns James P. 
Published: November 2023

AbstractTake‐all root rot (TARR) is a detrimental disease of ultradwarf bermudagrass (Cynodon dactylon × Cynodon transvaalensis; UDB) putting greens frequently diagnosed where warm‐season grasses are grown. Since this disease is largely aggregated and variable under field conditions, field research is difficult and often yields inconsistent results. Multiple pathogens have only recently been associated with this disease, so practical management solutions such as fungicide efficacy and fungicide application timing have not been thoroughly investigated. Therefore, the objectives of this research were to determine the influence of fungicide selection and fungicide application timing on take‐all root rot management under field and greenhouse conditions. In general, fungicides from the quinone outside inhibitor and/or demethylation inhibitor chemical classes provided the greatest reduction in take‐all root rot severity. Fungicide applications that were made when soil temperatures were between 77–86°F provided the greatest disease suppression. The in vivo greenhouse method developed in this research proved to be an efficient and consistent method to evaluate management practices such as fungicide efficacy on take‐all root rot. This research improves our understanding of fungicide efficacy and fungicide application timing for take‐all root rot management on ultradwarf bermudagrass.
Synergistic topological and supramolecular control of Diels‐Alder reactivity based on a tunable self‐complexing host‐guest molecular switch
Authors: Ribeiro Cédric , Cariello Michele , Malfait Aurélie , Bria Marc , Fournier David , Lyskawa Joël , Le Fer Gaëlle , Potier Jonathan , Hoogenboom Richard , Cooke Graeme , Woisel Patrice 
Published: November 2023

AbstractCompartmentalization and binding‐triggered conformational change regulate many metabolic processes in living matter. Here, we have synergistically combined these two biorelevant processes to tune the Diels‐Alder (DA) reactivity of a synthetic self‐complexing host‐guest molecular switch CBPQT4+‐Fu, consisting of an electron‐rich furan unit covalently attached to the electron‐deficient cyclobis(paraquat‐p‐phenylene) tetrachloride (CBPQT4+, 4Cl−) host. This design allows CBPQT4+‐Fu to efficiently compartmentalize the furan ring inside its host cavity in water, thereby protecting it from the DA reaction with maleimide. Remarkably, the self‐complexed CBPQT4+‐Fu can undergo a conformational change through intramolecular decomplexation upon the addition of a stronger binding molecular naphthalene derivative as a competitive guest, triggering the DA reaction upon addition of a chemical regulator. Remarkably, connecting the guest to a thermoresponsive lower critical solution temperature (LCST) copolymer regulator controls the DA reaction on command upon heating and cooling the reaction media beyond and below the cloud point temperature of the copolymer, representing a rare example of decreased reactivity upon increasing temperature. Altogether, this work opens up new avenues towards combined topological and supramolecular control over reactivity in synthetic constructs, enabling control over reactivity through molecular regulators or even mild temperature variations.
Implementation of the irregular pulse density modulation‐controlled wireless power transfer system for constant current and constant voltage output
Authors: Unal Kenan , Bal Gungor , Oncu Selim 
Published: November 2023

SummaryIn this paper, irregular 32 pulse density modulation (PDM)‐controlled wireless power transfer (WPT) system is proposed for constant current (CC) and constant voltage (CV) outputs. The proposed WPT system has a load independent ZPA frequency characteristic with CC and CV outputs. Thus, soft switching conditions are achieved for both CC and CV outputs. An experimental prototype was prepared to validate the effectiveness of the proposed irregular 32 PDM‐controlled WPT system. WPT coil design parameters are presented and analyzed with lateral and rotational misalignment conditions in ANSYS. The experiments were determined as 3 A for CC output and 200 V for CV output. The maximum efficiency of the proposed system is measured (95.34%) at aligned coils with 100 mm air gap. In addition, the efficiency results are presented and evaluated by performing experimental studies under both lateral and rotational misalignment conditions.
Two‐port dielectric resonator antenna loaded with meta‐surface for improving isolation and circular polarization generator
Authors: Gupta Vivek Kumar , Alam Monauwer , Yadav Abhimanyu , Sharma Anand 
Published: November 2023

SummaryIn this communication, a dielectric resonator based dual‐port radiator is designed. Loading of meta‐surface over the dual‐port antenna creates two important features that make it unique to the proposed design: (i) conversion of the linear polarized plane waves into circularly polarized plane wave (ii) improve the isolation between the antenna port by more than 30 dB. Experimental measurement confirms that proposed design is working in the frequency range 5.14–5.55 GHz, and a 3‐dB axial ratio is produced from 5.36 to 5.45 GHz. Stable radiation pattern and good value of diversity performance are also achieved in the proposed radiator. All of these characteristics make the developed radiator appropriate for WLAN (5.4 GHz) applications.
Application of micro capillary blood sampling in an anti‐doping setting
Authors: Lewis Laura , Goodrum Jenna , Cai Calvin , Muir Tessa , Boutard Kristin , Capdevielle Thomas , Longo Stefano , Fedoruk Matthew , Miller Geoff 
Published: November 2023

AbstractBlood collection is an important facet of anti‐doping testing, forming the basis of the Athlete Biological Passport (ABP). Traditional blood collection via venipuncture can be uncomfortable for athletes, especially those who are tested frequently or close to competition. Athletes may also have negative perceptions of venipuncture due to past experiences or the risks of adverse health events such as bruising, hematomas, syncope, and general discomfort that has the potential to affect performance. Advances in capillary whole blood collection technology now affords the ability to collect micro‐volumetric capillary whole blood from the upper arm (or other suitable vascular location such as the abdomen) that is “needle‐free” and virtually painless using devices such as the Tasso+. The present study extends previous work, by collecting microliter capillary whole blood samples via the Tasso+ EDTA device in an official anti‐doping setting prior to competition, as well as requiring temperature‐monitored cold chain shipping by air to the laboratory before analysis. Fifty‐eight matched capillary and venous blood samples were collected under official doping control conditions by certified Doping Control Officers. No impact of sample shipment by air under cool conditions was observed on sample integrity. Provided that no visible clots were identified prior to analysis, capillary and venous blood samples showed excellent laboratory agreement for all CBC parameters, with the exception of platelets. Micro capillary blood collection provides a valid alternative to venous blood collection for ABP purposes, with the advantage of providing a more athlete‐friendly experience, particularly close to competition.
An enhanced label‐free proteomics approach for deep‐diving into equine plasma proteome, including the discovery of protein biomarkers for strenuous exercise
Authors: Cheung Hiu Wing , Wong Kin‐Sing , To Ning Sum , Wan Terence S. M. , Ho Emmie N. M. 
Published: November 2023

AbstractPlasma proteins have been a valuable source of biomarkers for clinical uses and for monitoring of the illicit use of prohibited substances or practices in equine sports. We have previously reported the first use of label‐free proteomics in profiling equine plasma proteome. This study aimed to refine the method by systematically evaluating various plasma fractionation methods and the use of narrower precursor mass ranges in data‐independent acquisition (DIA) mass spectrometry (MS). Tandem fractionations of equine plasma with octanoic acid precipitation followed by solid‐phase extraction (SPE) with C4 cartridges provided the largest increase in the number of new proteins identified. The use of two narrow precursor mass ranges of m/z 400–600 and 600–800 in DIA not only identified most proteins detectable by using a single mass range of m/z 350–1500 but also identified ~27% more proteins. The improved method was applied to analyse the plasma proteome of ‘postrace’ samples which, unlike other samples, had been collected from racehorses soon after racing. Multivariate data analysis has identified upregulation of 14 proteins and downregulation of six proteins in postrace plasma compared with the non‐postrace plasma samples. Literature review of these proteins has provided evidence of exercise‐induced haemolysis and changes in antioxidant enzyme activities, kinin system, insulin signalling and energy metabolism after strenuous exercise. The improved method has enabled a deeper profiling of the equine plasma proteome and identified the proteins associated with normal physiological changes after racing which are potential confounding factors in the development of a biomarker approach for doping control.
Navigating Informed Consent Requirements and Expectations in Cluster Randomized Trials: Research Ethics Board Members’ and Researchers’ Views
Authors: Ho Anita , Joolaee Soodabeh , McDonald Michael , Grant Don , White Michel M. , Longstaff Holly , Palsson Eirikur 
Published: November 2023

ABSTRACTInformed consent is a cornerstone of ethical human research. However, as cluster randomized trials (CRTs) are increasingly popular to evaluate health service interventions, especially as health systems aspire toward the learning health system, questions abound how research teams and research ethics boards (REBs) should navigate intertwining consent and data‐use considerations. Methodological and ethical questions include who constitute the participants, whose and what types of consent are necessary, and how data from people who have not consented to participation should be managed to optimize the balance of trust in the research enterprise, respect for persons, the promotion of data integrity, and the pursuit of the public good in the research arena. In this paper, we report the findings and lessons learned from a qualitative study examining how researchers and REB members consider the ethical dimensions of when data can be collected and used in CRTs in the evolving research landscape.
Should Chatbots Be Used to Obtain Informed Consent for Research?
Authors: Rothstein Mark A. 
Published: November 2023

ABSTRACTChatbots have become increasingly common in diverse settings as a substitute for human conversation. They are being developed and tested for obtaining informed consent for research. An initial study indicated that chatbots saved time and were successful in knowledge transfer, but the informed consent process serves other purposes, such as building trust and respecting the autonomy and dignity of potential research participants. Additional research and possible regulation are necessary before chatbots should be routinely used in health research.
Partitioning variance in reproductive success, within years and across lifetimes
Authors: Waples Robin S. 
Published: November 2023

AbstractVariance in reproductive success (, with k = number of offspring) plays a large role in determining the rate of genetic drift and the scope within which selection acts. Various frameworks have been proposed to parse factors that contribute to , but none has focused on age‐specific values of , which indicate the degree to which reproductive skew is overdispersed (compared to the random Poisson expectation) among individuals of the same age and sex. Instead, within‐age effects are generally lumped with residual variance and treated as “noise.” Here, an ANOVA sums‐of‐squares framework is used to partition variance in annual and lifetime reproductive success into between‐group and within‐group components. For annual reproduction, the between‐age effect depends on age‐specific fecundity (bx), but relatively few empirical data are available on the within‐age effect, which depends on ϕx. By defining groups by age‐at‐death rather than age, the same ANOVA framework can be used to partition variance in lifetime reproductive success (LRS) into between‐group and within‐group components. Analytical methods are used to develop null‐model expectations for random contributions to within‐group and between‐group components. For analysis of LRS, random variation in longevity appears as part of the between‐group variance, and effects (if any) of skip breeding and persistent individual differences contribute to the within‐group variance. Simulations are used to show that the methods for variance partitioning are asymptotically unbiased. Practical application is illustrated with empirical data for annual reproduction in American black bears and lifetime reproduction in Dutch great tits. Results show that overdispersed within‐age variance (1) dominates annual in both male and female black bears, (2) is the primary factor that reduces annual effective size to a fraction of the number of adults, and (3) represents most of the opportunity for selection. In contrast, about a quarter of the variance in LRS in great tits can be attributed to random variation in longevity, and most of the rest is due to modest differences in fecundity with age estimated for a single cohort of females. R code is provided that reads generic input files for annual and lifetime reproductive success and allows users to conduct variance partitioning with their own data.