bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Platelet-Rich Plasma at Different Initiation Times on Healing of the Bone-Tendon Interface of the Rotator Cuff in a Mouse Model
Authors: Peng Yundong , Diao Luyu , Wang Juan , Wang Guanglan , Jia Shaohui , Zheng Cheng 
Published: February 2024

Background: Platelet-rich plasma (PRP) has demonstrated beneficial effects on healing of the bone-tendon interface (BTI). Purpose: To determine the optimal initiation time for PRP application after rotator cuff repair in an animal model. Study Design: Controlled laboratory study. Methods: A total of 136 C57BL/6 mice were included; 40 mice were used to prepare PRP, while 96 mice underwent acute supraspinatus tendon (SST) repair. The animals were randomly divided into 4 groups: a control group and 3 groups in which PRP was injected into the injury interface immediately after surgery, on the 7th postoperative day (PRP-7d), and on the 14th postoperative day. At 4 and 8 weeks postoperatively, the animals were sacrificed, blood was collected by eyeball removal, and samples of the SST-humerus complex were collected. Histological, imaging, immunological, and biomechanical data were compared among the groups using 1-way analysis of variance with the Bonferroni post hoc test. Results: Histological analysis revealed that the fibrocartilage layer at the BTI was larger in the PRP-7d group compared to the other groups at both 4 and 8 weeks postoperatively. Moreover, the PRP-7d group exhibited improved proteoglycan content and distribution compared to the other groups. Enzyme-linked immunosorbent assay results demonstrated that at 4 weeks postoperatively, higher concentrations of transforming growth factor–β1 and platelet-derived growth factor–BB (PDGF-BB) were seen in the PRP-7d group versus the PRP-14d and control gruops ( P < .05), and at 8 weeks postoperatively, the concentration of PDGF-BB was higher in the PRP-7d group versus the control group ( P < .05). Biomechanical testing at 4 weeks postoperatively revealed that the failure load and ultimate strength of the SST-humerus complex were superior in the PRP-7d group compared to the other groups ( P < .05), at 8 weeks, PRP-7d group was superior to the control group ( P < .05). Additionally, at 8 weeks postoperatively, the PRP-7d group exhibited a greater trabecular number and trabecular thickness at the BTI compared to the PRP-14d and control gruops ( P < .05). Conclusion: PRP promoted healing of the BTI after a rotator cuff injury at an early stage. Clinical Relevance: A PRP injection on the 7th postoperative day demonstrated superior therapeutic effects compared with injections at other time points.
Validity of Material Related to the Anterior Cruciate Ligament on TikTok
Authors: D’Ambrosi Riccardo , Hewett Timothy E. 
Published: February 2024

Background: Video material is one of the most common types of digital information on social media platforms. Purpose: To assess the validity and informational value of TikTok videos with regard to the anterior cruciate ligament (ACL). Study Design: Cross-sectional study. Methods: Using the terms “anterior cruciate ligament” AND/OR “ACL,” 420 TikTok videos were retrieved, of which 100 videos were included in the analysis. The video duration and the number of likes, shares, and views were recorded. Videos were categorized based on the source (private user, physical therapist, surgeon, and researcher), subject type (patient experience, physical therapy, injury mechanism, anatomy, clinical test, and surgical technique), content (patient experience/testimony, education, and rehabilitation), and any background audio (music or voice). Video quality assessments were conducted using the DISCERN instrument, The Journal of the American Medical Association ( JAMA) benchmark criteria, and the Global Quality Score (GQS). Associations between quantitative variables were tested using the Spearman rank correlation. One-way analysis of variance or Mann-Whitney test was performed to assess whether video quality differed by video characteristics. Results: A total of 41 videos were published by private users (41%), 34 by physical therapists (34%), 23 by surgeons (23%), and 2 by researchers (2%). Most of the information regarded patient experience (34%), followed by physical therapy (20%), injury mechanism (19%), anatomy (11%), clinical test (9%), and surgical technique (7%). The mean video length was 40.55 ± 41.58 seconds. The mean number of views was 151,084.39 ± 487,150.02, while the mean numbers of comments, likes, and shares were 72.80 ± 249.68, 6781.49 ± 29,163.96, and 98.71 ± 307.76, respectively. The mean DISCERN, JAMA, and GQS scores were 15.73 ± 1.44, 0.19 ± 0.39, and 1.16 ± 0.37, respectively, indicating the poorest quality on all 3 indices. The number of views, likes, shares, comments, and video lengths were all positively correlated with DISCERN (except the number of shares), JAMA, and GQS scores ( P < .05). A significant difference was found in DISCERN scores between videos by private users and those by surgeons (15.37 ± 0.98 vs 16.22 ± 1.57; P = .007). Conclusion: Our analysis has demonstrated that the educational value of these videos on the ACL on TikTok is poor. Given the rapid growth of TikTok, further research is needed.
Modification of thermal and electrical characteristics of hybrid polymer nanocomposites through gamma irradiation for advanced applications
Authors: Kavitha C. M. , Eshwarappa K. M. , Shetty Shivakumar Jagadish , Gurumurthy S. C. , Surabhi Srivathsava , Niranjana Prabhu T. , Jeong Jong-Ryul , Morales D. V. 
Published: February 2024

AbstractIn this article, we present a straightforward in-situ approach for producing Ag NPs incorporated in graphene oxide (GO) blended with glutaraldehyde (GA) cross-linked polyvinyl alcohol (PVA) matrix. Samples are γ-irradiated by doses of 2, 5, and 10 kGy and in comparison with the pristine films, the thermal conductivity (‘k’) and effusivity are measured. ‘k’ decreases with irradiation doses up to 5 kGy and further increase in the dosage results increase in ‘k’. We performed FDTD modeling to verify the effect of polarization and periodicity on the absorptivity and emissivity spectra that are correlated to the ‘k’ and effusivity, empirically. Hence, we can confess that the structural properties of the prepared hybrid nanocomposite are manipulated by γ-irradiation. This attests that the PVA/GO-Ag/GA nanocomposite is radiation-sensitive and could be employed for thermal management systems. Moreover, their strong electrical insulation, as the measured dc conductivity of the γ-irradiated samples is found to be in the range of 2.66 × 10−8–4.319 × 10−7 Sm−1, which is below the percolation threshold of 1.0 × 10−6 Sm−1, demonstrates that they are excellent candidates for the use of thermal management materials. The low ‘k’ values allow us to use this promising material as thermal insulating substrates in microsensors and microsystems. They are also great choices for usage as wire and cable insulation in nuclear reactors due to their superior electrical insulation.
Graphical Abstract
Recent progress in mass spectrometry-based urinary proteomics
Authors: Joshi Neha , Garapati Kishore , Ghose Vivek , Kandasamy Richard K. , Pandey Akhilesh 
Published: February 2024

AbstractSerum or plasma is frequently utilized in biomedical research; however, its application is impeded by the requirement for invasive sample collection. The non-invasive nature of urine collection makes it an attractive alternative for disease characterization and biomarker discovery. Mass spectrometry-based protein profiling of urine has led to the discovery of several disease-associated biomarkers. Proteomic analysis of urine has not only been applied to disorders of the kidney and urinary bladder but also to conditions affecting distant organs because proteins excreted in the urine originate from multiple organs. This review provides a progress update on urinary proteomics carried out over the past decade. Studies summarized in this review have expanded the catalog of proteins detected in the urine in a variety of clinical conditions. The wide range of applications of urine analysis—from characterizing diseases to discovering predictive, diagnostic and prognostic markers—continues to drive investigations of the urinary proteome.
Genome-wide detection of positive and balancing signatures of selection shared by four domesticated rainbow trout populations (Oncorhynchus mykiss)
Authors: Paul Katy , Restoux Gwendal , Phocas Florence 
Published: February 2024

Abstract
Background
Evolutionary processes leave footprints along the genome over time. Highly homozygous regions may correspond to positive selection of favorable alleles, while maintenance of heterozygous regions may be due to balancing selection phenomena. We analyzed data from 176 fish from four disconnected domestic rainbow trout populations that were genotyped using a high-density Axiom Trout genotyping 665K single nucleotide polymorphism array, including 20 from the US and 156 from three French lines. Using methods based on runs of homozygosity and extended haplotype homozygosity, we detected signatures of selection in these four populations.

Results
Nine genomic regions that included 253 genes were identified as being under positive selection in all four populations Most were located on chromosome 2 but also on chromosomes 12, 15, 16, and 20. In addition, four heterozygous regions that contain 29 genes that are putatively under balancing selection were also shared by the four populations. These were located on chromosomes 10, 13, and 19. Regardless of the homozygous or heterozygous nature of the regions, in each region, we detected several genes that are highly conserved among vertebrates due to their critical roles in cellular and nuclear organization, embryonic development, or immunity. We identified new candidate genes involved in rainbow trout fitness, as well as 17 genes that were previously identified to be under positive selection, 10 of which in other fishes (auts2, atp1b3, zp4, znf135, igf-1α, brd2, col9a2, mrap2, pbx1, and emilin-3).

Conclusions
Using material from disconnected populations of different origins allowed us to draw a genome-wide map of signatures of positive selection that are shared between these rainbow trout populations, and to identify several regions that are putatively under balancing selection. These results provide a valuable resource for future investigations of the dynamics of genetic diversity and genome evolution during domestication.
Analgesic effect of the ultrasound-guided thoracolumbar paravertebral block in patients undergoing robot-assisted laparoscopic nephrectomy: a randomized controlled trial
Authors: Yin Guojiang , Li Yue , Wei Pengxiao , Ma Xuyuan , Li Bixi , Gan Guosheng , Song Xiaoyang 
Published: February 2024

Abstract
Background
Paravertebral block has similar effect as epidural anesthesia, and has good somatic and visceral analgesic effect. Paravertebral block is widely used in thoracic surgery, but rarely used in abdominal surgery.

Aims
This study aimed to evaluate the analgesic effect of thoracolumbar paravertebral block in patients undergoing robot-assisted laparoscopic nephrectomy.

Methods
One hundred patients undergoing elective robot-assisted laparoscopic nephrectomy were included in this study. Based on whether the thoracolumbar paravertebral block was performed, the patients were randomly divided into the thoracolumbar paravertebral block combined with general anesthesia group (TL-PVB group) and simple general anesthesia group (NO-PVB group). Oxycodone was administered for patient-controlled intravenous analgesia (PCIA). The primary outcomes included the amount of remifentanil used during surgery, the amount of oxycodone used in 24 and 48 h after surgery. Secondary outcomes included the changes of heart rate (HR) and mean arterial pressure (MAP), time for the first analgesia administration, visual analog score (VAS) of pain during rest and movement, and time of postoperative recovery.

Results
Compared to the NO-PVB group, the amount of remifentanil used during surgery in patients with TL-PVB group was significantly reduced (1.78 ± 0.37 mg vs. 3.09 ± 0.48 mg, p < 0.001), the amount of oxycodone used 24 h after surgery was significantly reduced (8.70 ± 1.70 mg vs. 13.79 ± 2.74 mg, p < 0.001), and the amount of oxycodone used 48 h after surgery was remarkably reduced (21.83 ± 4.28 mg vs. 27.27 ± 4.76 mg, p < 0.001). There were significant differences in the changes of HR and MAP between the two groups (p < 0.001). The first analgesic requirement time of TL-PVB group was significantly longer than that of NO-PVB group (468.56 ± 169.60 min vs. 113.48 ± 37.26 min, p < 0.001). The postoperative VAS during rest and movement of TL-PVB group were significantly lower than that of NO-PVB group (p < 0.01). Compared with NO-PVB group, patients in TL-PVB group needed shorter time to awaken from anesthesia, leave the operating room, anal exhaust, get out of bed, and had shorter length of postoperative hospital stay (p < 0.001). The incidence of postoperative adverse reactions were lower in the TL-PVB group than that in the NO-PVB group (p < 0.05).

Conclusions
Ultrasound-guided thoracolumbar paravertebral block significantly reduces intraoperative and postoperative opioid consumption, and provides better analgesia in patients undergoing robot-assisted laparoscopic nephrectomy, which is a recommendable combined anesthesia technique.

Trial registration
ChiCTR2200061326, 21/06/2022.
Combined score based on plasma fibrinogen and platelet-lymphocyte ratio as a prognostic biomarker in esophageal squamous cell carcinoma
Authors: Yang Yuchong , Tan Hui , Lu Yao , Mei Jipeng , Zhang Mengqi , Bai Ming , Wang Xia , Ge Shaohua , Ning Tao , Zhang Le , Ji Zhi , Duan Jingjing , Sun Yansha , Wang Feixue , Liu Rui , Li Hongli , Deng Ting 
Published: February 2024

Abstract
Background
Increasing evidence has showed that inflammatory biomarkers, including neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and fibrinogen can be used as predictors in the prognosis of esophageal squamous cell carcinoma (ESCC). The aim of this study was to explore prognostic value of these biomarkers and evaluate the clinicopathological and prognostic significance of combined score based on plasma fibrinogen and platelet-lymphocyte ratio (F-PLR score).

Methods
A total of 506 patients with ESCC were enrolled in this study. Harrell’s concordance index (c-index) was used to determine the optimal cut-off values of these markers and evaluate their prognostic significance. The relationship between factors with survival rates (including overall survival [OS] and disease-free survival [DFS]) was explored by Kaplan-Meier curve, univariate analysis and multivariate cox hazard analysis.

Results
Our result indicated that high F-PLR score was significantly associated with longer tumor length and deeper depth of tumor invasion (p < 0.01). The result of Cox multivariable analysis showed that F-PLR score was an independent prognostic factor for OS (p = 0.002) and DFS (p = 0.003). In addition, F-PLR score presented the greater c-index values for OS and DFS compared with NLR, PLR and fibrinogen level. Our result also showed that the c-index values for OS and DFS were both greater in TNM + F-PLR than those in TNM stage alone.

Conclusions
In conclusion, F-PLR score is a predictive biomarker for prognosis in patients with ESCC.
5-Methoxytryptophan enhances the sensitivity of sorafenib on the inhibition of proliferation and metastasis for lung cancer cells
Authors: Chen Huang-Chi , Kuo Chia-Yu , Chang Yu , Tsai Dong-Lin , Lee Mei-Hsuan , Lee Jui-Ying , Lee Hui-Ming , Su Yu-Chieh 
Published: February 2024

Abstract
Background
Lung cancer is a leading cause of cancer-related mortality worldwide, and effective therapies are limited. Lung cancer is a leading cause of cancer-related mortality worldwide with limited effective therapy. Sorafenib is a multi-tyrosine kinase inhibitor frequently used to treat numerous types of malignant tumors. However, it has been demonstrated that sorafenib showed moderate antitumor activity and is associated with several side effects in lung cancer, which restricted its clinical application. This study aimed to examine the antitumor effect of the combination treatment of sorafenib and 5-methoxytryptophan (5-MTP) on cell growth and metastasis of Lewis lung carcinoma (LLC) cells.

Method
The anticancer effect of the combination treatment of sorafenib and 5-MTP was determined through cytotoxicity assay and colony forming assays. The mechanism was elucidated using flow cytometry and western blotting. Wound healing and Transwell assays were conducted to evaluate the impact of the combination treatment on migration and invasion abilities. An in vivo model was employed to analyze the effect of the combination treatment on the tumorigenic ability of LLC cells.

Result
Our results demonstrated that the sorafenib and 5-MTP combination synergistically reduced viability and proliferation compared to sorafenib or 5-MTP treatment alone. Reduction of cyclin D1 expression was observed in the sorafenib alone or combination treatments, leading to cell cycle arrest. Furthermore, the sorafenib-5-MTP combination significantly increased the inhibitory effect on migration and invasion of LLC cells compared to the single treatments. The combination also significantly downregulated vimentin and MMP9 levels, contributing to the inhibition of metastasis. The reduction of phosphorylated Akt and STAT3 expression may further contribute to the inhibitory effect on proliferation and metastasis. In vivo, the sorafenib-5-MTP combination further reduced tumor growth and metastasis compared to the treatment of sorafenib alone.

Conclusions
In conclusion, our data indicate that 5-MTP sensitizes the antitumor activity of sorafenib in LLC cells in vitro and in vivo, suggesting that sorafenib-5-MTP has the potential to serve as a therapeutic option for patients with lung cancer.
Morphological and functional characteristics of the meibomian gland in pediatric patients with epiblepharon
Authors: Li Junping , Qin Xiaolin , Jiang Hong , Guo Yunan , Liu Xin , Zhang Rui , Jiang Lu , Du Anshi 
Published: February 2024

Abstract
Background
To observe morphologic and functional changes in meibomian glands in pediatric patients with and without lower eyelid epiblepharon.

Methods
In this prospective observation study, 55 eyes of 55 patients( 24 males, 31 females; mean age ± SD,9.82 ± 2.59 years; range 6–14 years) and 60 eyes of 60 controls ( 32 males, 28 females; mean age ± SD,10.57 ± 2.75 years; range 6–14 years) were included. The following tests were performed: eyelid margin abnormality by slit-lamp examination, measurement of noninvasive keratographic break-up time (NIKBUT), grading of absence of meibomian gland (meibography score) assessed with noncontact meibography, morphologic changes of meibomian glands (thinning, dilatation and distortion), tear production by the Schirmer 1 test, and grading of meibum quality and meibomian gland expressibility.

Results
The morphologic changes in meibomian glands were more common in the epiblepharon group (56.36%) than in the control group (28.33%) (p = 0.002). The meibum quality was worse in the epiblepharon group than in the control group (p = 0.009), and the NIKBUT was significantly shorter in the epiblepharon group than in the control group (p = 0.012). There was no significant difference in the Schirmer 1 test, meibomian gland expressibility, eyelid margin abnormality score or total meibography score between the two groups. Morphologic changes in the meibomian glands in the upper eyelids (38.18%) were more common than those in the lower eyelids (20%) (p = 0.036) in the epiblepharon group, and the meibography score was higher in the upper eyelids than in the lower eyelids (p = 0.001).

Conclusion
There are morphological and functional changes in meibomian glands in pediatric patients with lower eyelid epiblepharon. Although the inverted eyelashes were located in the lower eyelid, morphological changes in the meibomian glands were more common in the upper eyelid.
Effect of repetitive transcranial magnetic stimulation-assisted training on lower limb motor function in children with hemiplegic cerebral palsy
Authors: He Yan , Zhang Qi , Ma Ting-Ting , Liang Yan-Hua , Guo Rong-Rong , Li Xiao-Song , Liu Qian-Jin , Feng Tian-Yang 
Published: February 2024

Abstract
Objective
To explore the effect of repetitive transcranial magnetic stimulation (rTMS)-assisted training on lower limb motor function in children with hemiplegic cerebral palsy (HCP).

Method
Thirty-one children with HCP who met the inclusion criteria were selected and randomly divided into a control group (n = 16) and an experimental group (n = 15). The control group received routine rehabilitation treatment for 30 min each time, twice a day, 5 days a week for 4 weeks. Based on the control group, the experimental group received rTMS for 20 min each time, once a day, 5 days a week for 4 weeks. The outcome measures included a 10-metre walk test (10MWT), a 6-minute walk distance (6MWD) test, D- and E-zone gross motor function measurements (GMFM), the symmetry ratio of the step length and stance time and the muscle tone of the triceps surae and the hamstrings (evaluated according to the modified Ashworth scale), which were obtained in both groups of children before and after treatment.

Results
After training, the 10MWT (P < 0.05), 6MWD (P < 0.01), GMFM (P < 0.001) and the symmetry ratio of the step length and stance time of the two groups were significantly improved (P < 0.05), there was more of an improvement in the experimental group compared with the control group. There was no significant change in the muscle tone of the hamstrings between the two groups before and after treatment (P > 0.05). After treatment, the muscle tone of the triceps surae in the experimental group was significantly reduced (P < 0.05), but there was no significant change in the control group (P > 0.05).

Conclusion
Repetitive TMS-assisted training can improve lower limb motor function in children with HCP.
Effectiveness of computer-based stress inoculation training (SIT) counseling approach on anxiety, depression, and stress of students with premenstrual syndrome
Authors: Zolfaghary Fatemeh , Adib-Rad Hajar , Nasiri‑Amiri Fatemeh , Faramarzi Mahbobeh , Pasha Hajar , gholinia-ahangar Hemmat 
Published: February 2024

AbstractBackgroundPremenstrual Syndrome (PMS) is a common public health issue affecting many women of reproductive age worldwide. This study has been designed to investigate of computer-based stress inoculation training (SIT) counseling approach on anxiety, depression, and stress of university students with PMS.MethodsA randomized trial study with two parallel arms was done from 30 October 2022 to 21 June 2023 on 100 university students aged 18 to 38 at Babol University of Medical Sciences. The participants were randomly divided into two groups intervention and control. The data collection tools included questionnaires on demographic-fertility characteristics, the Premenstrual Symptoms Screening Tool (PSST), the Hospital Anxiety and Depression Scale (HADS), the Perceived Stress Scale (PSS-14), the Sheehan Disability Scale (SDS) and Riff’s Psychological Well-being Scale (RPWS). The data were assessed using chi-square, t-student, ANOVA repeated measure, and linear regression tests. A significance level ofP < 0.05 was considered for the analysis.ResultsThe results of the study showed that the SIT interventions decreased the PMS severity and most psychological factors so in the intervention group, SIT was able to significantly reduce anxiety, depression, perceived stress, and Sheehan’s disability after intervention (P < 0.001). Based on multiple linear regression analysis, the most predictors of HADS were the PSS and SDS (β = 0.285,p = 0.009 and β = 0.236,p = 0.024, respectively).ConclusionThe computer-based SIT counseling approach could reduce the severity of symptoms and psychological factors in students. Therefore, SIT intervention is recommended to manage their PMS.Trial registrationIRCT20230130057274N2.