bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ursolic acid restores redox homeostasis and proinflammatory cytokines production in denervation-induced skeletal muscle atrophy
Authors: Yadav Aarti , Dabur Rajesh 
Published: October 2023

Abstract

Skeletal muscle (SkM) atrophy results from metabolic disorders causing body and muscle mass loss, affecting morbidity and mortality. Increased oxidative stress, inflammation, and poor prognosis are the leading causes of involuntary weight loss. Ursolic acid (UA), known for its antioxidant and anti-inflammatory properties, can potentially reduce oxidative stress and inflammation in muscles, but its effects on muscle mass regulation are still unknown. Therefore, present study investigated the medicinal efficacy of UA and its mode of action against murine model of SkM atrophy over 7 days of UA supplementation. Denervation-induced SkM atrophy significantly impacts both overall body weight and the weight of individual muscles (p < 0.05). However, supplementation with UA can effectively counteract these effects by promoting the synthesis of the slow-myosin heavy chain, thereby restoring body weight and myotube diameter. Moreover, UA also plays a crucial role in reducing the production levels of reactive oxygen species (ROS), lipid peroxidation (LPO), and caspase-3-like activity in atrophied muscles. UA also, prevents the leakage of creatine kinase (CK) through the upregulation of superoxide dismutase (SOD) and glutathione peroxidase (GPx) expression. Furthermore, the results obtained from qRT-PCR demonstrated a significant decrease in the levels of proinflammatory markers, namely IL-1β, IL-6, TNF-α, and TWEAK, approximately four-fold after the third day of the UA intervention. UA also upregulated PGC-1α, Bcl2, and Akt expression for maintenance of redox homeostasis.
Psychometric Properties and Validity of the Pros and Cons of Anorexia Scale: An Exploration of Anorexia Patients’ Endorsement of Benefits and Burdens of Their Illness, and Severity, Subtype and Motivation
Authors: Gregertsen Eva Cecilie , Mandy William , Armstrong Stephanie , Kanakam Natalie , Frampton Ian , Hennessey Georgia , Sodeke-Gregson Ayo , Cardinal Annie , Robinson Paul , Serpell Lucy 
Published: October 2023

Abstract

Background The egosyntonic nature of anorexia nervosa (AN) – or valued nature of the illness – has been highlighted as a possible maintaining factor in AN. As such, understanding egosyntonicity is a key issue in both assessment and treatment of AN, and solid instruments capturing the perceived benefits and burdens of AN may be useful to clinicians. Accordingly, the current study analysed the psychometric properties of the Pros and Cons of Anorexia Scale (P-CAN), including its relationship with motivation and illness severity.Methods 100 female AN patients completed the P-CAN, Eating Disorder Examination (ED) Questionnaire, Anorexia Stages of Change Questionnaire, Readiness and Motivation Questionnaire, and Concerns about Change.Results The P-CAN had good psychometric properties. Significant correlations with scales measuring ED psychopathology indicated meaningful concurrent validity. Endorsement of pros, alongside endorsement of the “hatred” con, was linked with greater ED pathology, greater readiness to change, lesser confidence, and lesser concerns about change. Endorsement of cons was linked with lesser ED pathology, lesser readiness to change, and greater concerns about change. The relationship between endorsement of pros and greater ED pathology was mediated by motivation.Conclusions The P-CAN is a clinically useful instrument for measuring perceived pros and cons in individuals with AN.
YAP/TAZ interacts with RBM39 to confer resistance against indisulam
Authors: Ando Toshinori , Okamoto Kento , Shintani Tomoaki , Yanamoto Souichi , Miyauchi Mutsumi , Kajiya Mikihito 
Published: October 2023

Abstract

The Hippo pathway and its downstream effectors, Yes-associated protein/transcriptional coactivator with PDZ-binding motif (YAP/TAZ), are essential for cell growth and organ development. Emerging evidence revealed that the Hippo pathway and YAP/TAZ are frequently dysregulated by multiple genetic alterations in solid cancers including head and neck squamous cell carcinoma (HNSCC); however, the YAP/TAZ-nuclear interactome remains unclear. RNA-binding motif protein 39 (RBM39) enhances transcriptional activity of several transcription factors and also regulates mRNA splicing. Indisulam degrading RBM39 induce alternative splicing, leading to cell death. However, clinical trials of indisulam have failed to show the effectiveness. Therefore, clarifying the resistance mechanism against splicing inhibitors is urgently required. In this study, we identified RBM39 as a novel YAP/TAZ-interacting molecule by proteome analysis. RBM39 promoted YAP/TAZ transcriptional activity. We further elucidated that indisulam reduce RBM39/YAP/TAZ-mediated integrin or collagen expression, thereby inactivating focal adhesion kinase (FAK) important for cell survival. Moreover, indisulam also induced alternative splicing of cell cycle- or DNA metabolism-related genes. YAP/TAZ hyperactivation suppressed indisulam-induced RBM39 degradation, which restored the integrin/collagen expression to activate FAK, and alternative splicing, thereby conferring resistance against indisulam in vitro and in vivo. Our findings may aid to develop a novel cancer therapy focusing on YAP/TAZ/RBM39 interaction.
Environmental and sociocultural factors are associated with pain-related brain structure among diverse individuals with chronic musculoskeletal pain
Authors: Domenico Lisa , Tanner Jared , Mickle Angela , Terry Ellen , Garvan Cynthia , Lai Song , Deshpande Hrishikesh , Staud Roland , Redden David , Price Catherine , Goodin Burel , Fillingim Roger , Sibille Kimberley 
Published: October 2023

Abstract
Chronic musculoskeletal pain is a leading cause of disability worldwide. Previous research indicates ethnic/race groups are disproportionately affected by chronic pain conditions. However, when considering socioenvironmental factors these disparities are no longer observed. Ethnic/race group differences have also been reported in pain-related brain structure. Given that environmental and sociocultural factors influence biology and health outcomes, this study aimed to investigate possible environmental and sociocultural contributions to structural differences in pain-related brain regions. A total of 147 non-Hispanic black and non-Hispanic white, middle and older aged adults with knee pain in the past month and a brain MRI are included in the analyses. Individuals also provided information specific to health and pain history and environmental and sociocultural resources. In hierarchical multiple regression models, sociocultural and environmental factors explained 6%-37% of the variance in thickness of pain-related brain regions, with seven of the eight brain regions being statistically significant. In the amygdala, hippocampus, insula, bilateral primary somatosensory cortex, and thalamus, ethnicity/race provided an additional 4%-13% of explanatory value. In the rostral/caudal anterior cingulate and dorsolateral prefrontal cortex, ethnicity/race was not a predictor after accounting for environmental, sociocultural, and other demographic measures. Findings inform health disparities research by elucidating the complexity of factors contributing to previously reported ethnicity/race group differences.
Study on machining characteristics of magnetic field assisted micro-EDM with ultrasonic vibration of workpiece
Authors: Yu Peng , Gao Yongcheng , Zhang Xiaodong , Ren Wanfei , Li Yiquan , Xu Jinkai , Yu Huadong 
Published: October 2023

Abstract
Electro discharge machining (EDM) has become one of the most commonly used methods for deep micro hole drilling because of its advantages of no macro force and no limitation of material mechanical properties. However, the characteristics of small discharge energy and narrow discharge gap of deep micro hole drilling by EDM, which leads to the problems of removing erosion debris. The problem of unstable machining process and low efficiency of EDM hinders its application and development. In this paper, the technology of magnetic field assisted micro-EDM with ultrasonic vibration of workpiece is studied to realize high efficiency and quality machining of micro holes with large aspect ratio. The working fluid in the discharge gap will generate alternating pressure driven by the workpiece electrode under ultrasonic vibration, which will promote the discharge debris to be removed from the machining area. The working fluid in the discharge gap will generate alternating pressure driven by the workpiece electrode under ultrasonic vibration, which will promote the discharge debris to be removed from the machining area. Under the influence of electric field and magnetic field, the moving direction of charged particle deflects due to the joint action of Coulomb force and Lorentz force, which prolongs its moving path in the discharge gap. In the study of multi energy field composite machining performance, the most significant factors affecting the material removal rate in the process parameters are ultrasonic amplitude, magnetic induction intensity and peak current, among which the interaction between ultrasonic amplitude and peak current is the most significant. The results also show that carburization and metallization occur simultaneously on the EDM surface, and the hardness of the surface layer of titanium alloy EDM is greater than that of the substrate material. Ultrasonic vibration and magnetic field assisted micro-EDM can reduce the surface residual tensile stress and crack size.
Embryos undergoing delayed second mitosis exhibit a reduced blastocyst formation rate and a lower live birth even if third mitosis was completed on time
Authors: Dong Ran , Ma Shuiying , Li Cheng , Li Qing , zhao haibin , Li Lianjie , Xu Kai , Zhao Hui , Li Mei 
Published: October 2023

Abstract
Background: The phenomenon of day3 (D3) 8-cell embryos derived from day2 (D2) 2-cell embryos can occur in in vitro fertilization (IVF) laboratories. Abnormal cleavage patterns can predict delayed second mitosis. Here, we investigated the developmental potential of these embryos to provide a reference for the selection of embryos for transplantation.
Methods: Embryos were divided into four groups according to cell number on D2 and D3. Group A featured high-quality D2 2-cell embryos that developed into high-quality D3 8-cell embryos; group B featured the same type of embryos as group A except the embryos developed to compaction; group C featured high-quality D2 4-cell embryos that developed into D3 8-cell high quality embryos and group D featured the same type of embryos as group C except the embryos developed to compaction.
Results: The blastocyst formation rate of group A and group B was significantly lower than that of group C (P<0.001). With regards to D3 single embryo transfer (SET) and frozen single blastocyst transfer (SBT), the clinical pregnancy and live birth rates of group A were comparable with those of group C. For D3 double-embryo transfer (DET), the biochemical pregnancy rate, clinical pregnancy rate, and live birth rate, following the combined transfer of embryos from group A and Group C was significantly lower than that when transferring two embryos from group C.
Conclusions: Embryos with delayed second mitosis exhibited a lower blastocyst formation rate and poorer embryo outcomes even though third mitosis was completed on time. Blastocyst culture is useful for selecting blastocysts with a higher probability of live birth for embryos with delayed second mitosis.
Trial registration: This study is retrospective and does not require trial registration.
Clinicopathological analysis of mullerian adenosarcoma of 22 cases and the sequencing of DICER1 mutation
Authors: Yao Xiaohong , He Ying , Wang Wei 
Published: October 2023

Abstract
Background Mullerian adenosarcoma is a rare malignancy the diagnosis and treatment of adenosarcoma is often challenging. We analyzed the clinicopathological features of 22 adenosarcomas and DICER1 hot mutations were screened.
Methods Patients with adenosarcoma registered in the West China the second Hospital from year 2020 to June 2022 were examined. Somatic hotspot mutations in the RNase IIIb domain of DICER1 were screened in 22 adenosarcomas by Sanger sequencing.
Results Only one patient (case16) had DICER1 mutation. All of 22 patients performed total hysterectomy with bilateral salpingo-oophorectomy and 14/22 these patients received adjuvant treatment.
Conclusion No DICER1 hotspot mutations detected.Furthermore, 16/22 presents with early-stage tumors and have favorable outcome, whether overtreatment and adjuvant therapy is benefit for low-risk patients need more definitive data. Conservative management is possible in appropriately selected women with post-operatively intensive monitoring.
Predictive Value of qSOFA Score for Mortality in Older Patients with Dengue Fever : A Retrospective Case-control Study
Authors: Lee Chi-Heng , Ho Yu-Chieh , Hsu Chien-Chin , Lin Hung-Jung , Chung Jui-Yuan , Huang Chien-Cheng 
Published: October 2023

Abstract

Background:Dengue fever (DF) in older patients is associated with higher mortality, but optimal mortality prediction approaches remain unclear. We assessed the utility of the quick Sequential Organ Failure Assessment (qSOFA) as a predictive tool in older DF patients.Methods:We retrospectively included DF patients aged ≥65 years who visited our study hospital between September 1, 2015, and December 31, 2015. Demographics, vital signs, comorbidities, laboratory data, and mortality were collected. Accuracy of qSOFA score ≥2 in predicting 30-day mortality was evaluated.Results:626 older DF patients were recruited (mean age: 74.1 years, nearly equal sex distribution). Mean ± standard deviation of qSOFA score was higher in mortality group vs. survival group (0.6 ± 0.8 vs. 0.1 ± 0,p<0.01). Logistic regression showed qSOFA score ≥2 associated with higher mortality risk (odds ratio: 18.74; 95% confidence interval: 4.37−50.64). Adjusted area under receiver operating characteristic was 0.75, and Hosmer-Lemeshow goodness of fit test was 0.58. Sensitivity, specificity, positive predictive value, and negative predictive value of qSOFA score ≥2 for predicting mortality were 18.5%, 98.7%, 38.5%, and 96.4%, respectively.Conclusion:qSOFA score ≥2 is a simple and specific tool for predicting 30-day mortality in older patients with DF, with high specificity and negative predictive value. It can be used to rule out patients at high risk of mortality.
Comparison of Complete Campylobacter fetus Genomes Derived from Public Repositories
Authors: Paintsil Ellis Kobina , Adu-Asiamah Cynthia Kyerewaa , Akenten Charity Wiafe , Boahen Kennedy Gyau , Kwarteng Alexander , Berg Stefan , Danso Kwasi Obiri , Dekker Denise , Ofori Linda Aurelia 
Published: October 2023

Abstract

Background: Campylobacter fetus(Cf) causes reproductive problems in livestock and infections in immunocompromised individuals. The pathogenesis of Cf can be better understood by using bioinformatics tools to study their genomes. We report on comparative genomic analysis that includes all 23 publicly available complete genomes of C. fetus, as of 30 June 2023, collected from diverse sources across the globe.
Results: There was no overlap of ST among the Campylobacter fetussubsp. fetus (Cff), Campylobacter fetus subsp. venerealis(Cfv) and Campylobacter fetus subsp. testudinum (Cft) genomes. The majority (90.0%) of the C. fetus subsp. venerealis (Cfv) were ST-4. Although no phenotypicresistance was predicted, the Bacterial and Viral Bioinformatics Resource Center software identified a total of 635 antibiotic resistance genes, with gyrA, gyrB, MacA and MacB present in all analyzed genomes. One Cff strain (CFF09A980) from bovine harboured tetracycline and streptomycin resistance genes, tet(44) and ant(6)-Ib. AnotherCftstrain (772) isolated from a human host had the tetracycline gene, tet(o). A total of 185 virulence factors were identified from the 23 genomes with each genome having exactly eight virulence factors: flgC, fliQ, fliM, fliN, fliP, fliI, pseB, and cheY. There were 63 prophage elements detected, but only four were intact. There were no protein family exclusive to only one strain. Proteomic analysis showed that Cft strains Sp3 and 772 have the least percentage identity to the reference genome. Phylogenetic tree of the 23 Cf genomes clustered in two major groups; Cft strains clustered together while Cfv, Cfvi and Cff were on one major clade.
Conclusion: These findings provide greater insights into the degree of pathogenicity and genomic diversity of complete genomes of Cf.
Study on mechanical response and fracture characteristics of rock under full path unloading
Authors: Liu Gang , Wang Dongwei , Li Yingming , Xiao Fukun 
Published: October 2023

Abstract
When the underground roadway of the coal mine passes through the abnormal area of geological structure (fault, collapse column, fracture zone, etc.), the excavation induces the unloading instability of engineering rock mass. The rock mass in the fracture zone is in elastic, plastic, and post-peak stress states. The process of excavation through the fracture zone is essentially unloading from the full stress path. To explore the mechanical response to sandstone under different stress levels, based on the study of the possibility of stress path, the elastic axial pressure unloading confining pressure, the elastic principal stress constant unloading confining pressure, the plastic axial pressure unloading confining pressure, the plastic principal stress constant unloading confining pressure, the plastic axial D1 displacement constant unloading confining pressure, the plastic axial pressure confining pressure proportional unloading, and the post-peak axial pressure confining pressure synchronous unloading path are systematically carried out. The characteristics of a full stress-strain curve under 7 unloading paths are obtained, the deformation law caused by unloading is analyzed, and the relationship between the unloading path and strain increment is studied. It is obtained that with the increase of unloading degree, the unloading deformation modulus E in the elastic stress state first increases, then stabilizes, and then decreases, and the unloading deformation modulus E in the plastic stress state gradually decreases, Poisson's ratio µ both showed an increasing trend, normalized plastic shear strain \({\gamma ^p}/\gamma _{{\hbox{max} }}^{p}\) and dilatancy angle ψ according to the single exponential function, the initial confining pressure is negatively correlated with the dilatancy angle, and the research results can support the enrichment and development of unloading rock mechanics.
Development and initiation of a preceptor program to improve midwifery and nursing clinical education in sub-Saharan Africa: Protocol for a mixed methods study
Authors: de Water Brittney van , Renning Kelsey , Nyondo Anda , Sonnie Mustapha , Longacre Ashley , Ewing Helen , Fullah Mary , Chepuka Lignet , Mann Julie 
Published: October 2023

Abstract
Background:Expanding the quality and quantity of midwifery and nursing clinical preceptors is a critical need in many sub-Saharan educational settings to strengthen students’ clinical learning outcomes, and ultimately to improve maternal and child mortality. Therefore, this study was developed to establish a year-long, four step, precepting program to 1) improve partnership building and program development 2) provide an evidence-based course to expand competency and confidence in precepting students 3) select preceptors to become train the trainers and 4) secure accreditation for the program, ultimately to engage and support cohorts of preceptors and continue to monitor and evaluate the effectiveness of the program over five years.
Methods:Qualitative and quantitative approaches will be used including evaluation of preceptors, faculty and leadership involved with the program, as well as students taught by preceptors. Data will include validated self-assessment scales, objective structured clinical examinations (OSCEs), satisfaction surveys, and direct clinical observation, in-depth interviews and/or focus group discussions (preceptors); feedback forms (students); process mapping and organizational readiness for implementing change surveys (faculty and leadership). Median change in scores will be the primary outcome for quantitative data. Content analysis within a deductive framework to identify key implementation and adoption themes will be used for qualitative data analysis.
Discussion:This study aims to assess the readiness and early effectiveness for implementing a preceptor program for midwifery and nursing in Sierra Leone and Malawi. Determining the effectiveness of this program will guide future adaptations in order to strengthen the program for sustainability and potential scale-up.
Auricular acupressure to reduce the incidence of adverse reactions to colonoscopic bowel preparation: a randomized controlled trial protocol
Authors: Zhang Jiahui , Ye Huizhen , Mo Yan , Zhang Haiyan , Zhang Beiping , Hu Xuejun , Zhong Cailing 
Published: October 2023

Abstract
Background: During colonoscopic bowel preparation, intolerance symptoms such as abdominal pain and nausea negatively impact patient compliance and the quality of bowel preparation. Chinese auricular acupressure could alleviate this phenomenon and promote gastrointestinal function. This trial aims to evaluate the efficacy and safety of auricular acupressure for reducing the incidence of adverse reactions during bowel preparation.
Methods: A total of 190 inpatients will be included in this parallel-group randomized controlled trial and will be split equally between the control and intervention groups. The intervention group will receive auricular acupressure during bowel preparation, whereas the control group will receive no additional treatm ent. The total incidence of adverse reactions to bowel preparation is the primary variable, while secondary outcome measures include the incidence and corresponding score of various adverse events, patientwillingness to participate in a future colonoscopy, laxative ingestion time, 100% laxative intake rate, and bowel preparation quality. Analyses will be performed using both intention-to-treat and per-protocol methods. The effects of the intervention will be evaluated by comparing the outcomes between the two arms.
Discussion: This study will provide objective evidenceto evaluate the efficacy and safety of auricular acupressure on the adverse reactionsof bowel preparation providing an important reference for larger trials in the future
Trial registration number: ChiCTR2200061742