bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bagaimanakah Senam Diabetes Dapat Mempengaruhi Kadar Gula Darah Pada Pasien Diabetes Melitus Di Kelurahan Kedungsari?
Authors: Masithoh Robiul Fitri , Pinilih Sambodo Sriadi 
Published: November 2023

Latar belakang: Prosentase kejadian diabetes mellitus yang dapat dicegah sekitar 80%. Diabetes merupakan penyebab utama kebutaan, serangan jantung, stroke, gagal ginjal dan amputasi kaki. Estimasi jumlah pasien diabetes mellitus diseluruh dunia peregional di tahun 2015 dan 2040 dengan usia terbanyak adalah 20-79 tahun. Upaya yang bisa dilakukan adalah salah satunya dengan aktivitas fisik (senam diabetes) melakukan diet sehat dengan memperbanyak tinggi serat. Tujuan penelitian: untuk mengetahui pengaruh modifikasi senam diabetes dan diet tinggi serat terhadap kadar gula darah pada pasien dengan diabetes mellitus di kelurahan Kedungsari Metodologi penelitian: Metode penelitian meliputi desain penelitian, populasi dan sampel, tempat penelitian dan waktu penelitian, pengumpulan data, uji validitas dan reliabilitas instrumen, etika penelitian dan analisa data. Desain penelitian yang digunakan adalah penelitian quasi eksperimen dengan desain pre-post test control group design, menggunakan 2 (dua) kelompok, yaitu kelompok eksperimen dan kelompok kontrol. kelompok eksperimen dilakukan senam diabetes 4 (hari) hari berturut-turut, selanjutnya pada kelompok kontrol melakukana aktivitas sehari hari. Sebelum dan sesudah intervensi dilakukan kembali pengukuran kadar gula darah pada kedua kelompok. Hasil Penelitian ada perbedaan kadar gula darah pada kelompok intervensi dan kelompok kontrol setelah dilakukan perlakuan sebanyak 4 hari dengan menggunakan gerakan kontiyu.
Can predatory mites aid in the management of the citrus leprosis mite?
Authors: Vechia Jaqueline F. Della , Andrade Daniel J. , Tassi Aline D. , Roda Amy , van Santen Edzard , Carrillo Daniel 
Published: November 2023

IntroductionThe flat mite Brevipalpus yothersi is the main vector of citrus leprosis in South and Central America and Mexico, where vector suppression using conventional acaricides is usually the only disease control method, leading to problems with acaricide resistance. This mite is present in Florida, where several viruses known to cause citrus leprosis were recently detected. This research builds preparedness for managing emerging viral diseases and mitigating potential problems associated with acaricide resistance by addressing the compatibility of chemical and biological control strategies against B. yothersi. The predatory mite Amblyseius largoensis has shown promise as a biological control agent of B. yothersi. However, the effects of acaricides used in citrus on this predator are unknown.MethodsThis study investigated the impact of different routes of acaricide exposure on A. largoensis, including direct contact, pesticide-contaminated prey, residual contact, and combined exposure routes in laboratory settings. Additionally, the efficacy of A. largoensis in controlling B. yothersi, alone and in combination with acaricide applications, was evaluated under greenhouse conditions.Results and discussionCommonly used acaricides like abamectin and spirodiclofen were highly effective in controlling B. yothersi and only slightly harmful to A. largoensis. Other acaricides were also effective against B. yothersi but were less compatible with the predatory mite. In the greenhouse trial, all acaricides, alone or in combination with the predators, and the predators alone, effectively suppressed B. yothersi one month after treatment application. However, B. yothersi populations significantly increased one month later in the abamectin-alone and predator-alone treatments. At the same time, the combination of abamectin and predators provided B. yothersi suppression throughout the experiment. The fenpyroximate-predator combination also provided better control than each treatment applied independently. All other acaricides provided prolonged B. yothersi control, hindering biological control probably due to the lack of prey for A. largoensis. Research findings highlight the potential for enhancing the selectivity of acaricides towards A. largoensis by manipulating their exposure route. Combining predatory mites with acaricides shows promise in improving B. yothersi management.
Bayesian denoising algorithm dealing with colored, non-stationary noise in continuous glucose monitoring timeseries
Authors: Camerlingo Nunzio , Siviero Ilaria , Vettoretti Martina , Sparacino Giovanni , Del Favero Simone , Facchinetti Andrea 
Published: November 2023

Introduction: The retrospective analysis of continuous glucose monitoring (CGM) timeseries can be hampered by colored and non-stationary measurement noise. Here, we introduce a Bayesian denoising (BD) algorithm to address both autocorrelation of measurement noise and temporal variability of its variance.Methods: BD utilizes adaptive, a-priori models of signal and noise, whose unknown variances are derived on partially-overlapped CGM windows, via smoothing approach based on linear mean square estimation. The CGM signal and noise variability profiles are then reconstructed using a kernel smoother. BD is first assessed on two simulated datasets, DS1 and DS2. On DS1, the effectiveness of accounting for colored noise is evaluated by comparison against a literature algorithm; on DS2, the effectiveness of accounting for the noise variance temporal variability is evaluated by comparison against a Butterworth filter. BD is then evaluated on 15 CGM timeseries measured by the Dexcom G6 (DR).Results: On DS1, BD allows reducing the root-mean-square-error (RMSE) from 8.10 [6.79–9.24] mg/dL to 6.28 [5.47–7.27] mg/dL (median [IQR]); on DS2, RMSE decreases from 6.85 [5.50–8.72] mg/dL to 5.35 [4.48–6.49] mg/dL. On DR, BD performs a reasonable tracking of noise variance variability and a satisfactory denoising.Discussion: The new algorithm effectively addresses the nature of CGM measurement error, outperforming existing denoising algorithms.
In vivo biocompatibility testing of nanoparticle-functionalized alginate–chitosan scaffolds for tissue engineering applications
Authors: Viveros-Moreno Nancy G. , Garcia-Lorenzana Mario , Peña-Mercado Eduardo , García-Sanmartín Josune , Narro-Íñiguez Judit , Salazar-García Marcela , Huerta-Yepez Sara , Sanchez-Gomez Concepción , Martínez Alfredo , Beltran-Vargas Nohra E. 
Published: November 2023

Background: There is a strong interest in designing new scaffolds for their potential application in tissue engineering and regenerative medicine. The incorporation of functionalization molecules can lead to the enhancement of scaffold properties, resulting in variations in scaffold compatibility. Therefore, the efficacy of the therapy could be compromised by the foreign body reaction triggered after implantation.Methods: In this study, the biocompatibilities of three scaffolds made from an alginate–chitosan combination and functionalized with gold nanoparticles (AuNp) and alginate-coated gold nanoparticles (AuNp + Alg) were evaluated in a subcutaneous implantation model in Wistar rats. Scaffolds and surrounding tissue were collected at 4-, 7- and 25-day postimplantation and processed for histological analysis and quantification of the expression of genes involved in angiogenesis, macrophage profile, and proinflammatory (IL-1β and TNFα) and anti-inflammatory (IL-4 and IL-10) cytokines.Results: Histological analysis showed a characteristic foreign body response that resolved 25 days postimplantation. The intensity of the reaction assessed through capsule thickness was similar among groups. Functionalizing the device with AuNp and AuNp + Alg decreased the expression of markers associated with cell death by apoptosis and polymorphonuclear leukocyte recruitment, suggesting increased compatibility with the host tissue. Similarly, the formation of many foreign body giant cells was prevented. Finally, an increased detection of alpha smooth muscle actin was observed, showing the angiogenic properties of the elaborated scaffolds.Conclusion: Our results show that the proposed scaffolds have improved biocompatibility and exhibit promising potential as biomaterials for elaborating tissue engineering constructs.
High-quality physiology of Alcanivorax borkumensis SK2 producing glycolipids enables efficient stirred-tank bioreactor cultivation
Authors: Karmainski Tobias , Dielentheis-Frenken Marie R. E. , Lipa Marie K. , Phan An N. T. , Blank Lars M. , Tiso Till 
Published: November 2023

Glycine-glucolipid, a glycolipid, is natively synthesized by the marine bacterium Alcanivorax borkumensis SK2. A. borkumensis is a Gram-negative, non-motile, aerobic, halophilic, rod-shaped γ-proteobacterium, classified as an obligate hydrocarbonoclastic bacterium. Naturally, this bacterium exists in low cell numbers in unpolluted marine environments, but during oil spills, the cell number significantly increases and can account for up to 90% of the microbial community responsible for oil degradation. This growth surge is attributed to two remarkable abilities: hydrocarbon degradation and membrane-associated biosurfactant production. This study aimed to characterize and enhance the growth and biosurfactant production of A. borkumensis, which initially exhibited poor growth in the previously published ONR7a, a defined salt medium. Various online analytic tools for monitoring growth were employed to optimize the published medium, leading to improved growth rates and elongated growth on pyruvate as a carbon source. The modified medium was supplemented with different carbon sources to stimulate glycine-glucolipid production. Pyruvate, acetate, and various hydrophobic carbon sources were utilized for glycolipid production. Growth was monitored via online determined oxygen transfer rate in shake flasks, while a recently published hyphenated HPLC-MS method was used for glycine-glucolipid analytics. To transfer into 3 L stirred-tank bioreactor, aerated batch fermentations were conducted using n-tetradecane and acetate as carbon sources. The challenge of foam formation was overcome using bubble-free membrane aeration with acetate as the carbon source. In conclusion, the growth kinetics of A. borkumensis and glycine-glucolipid production were significantly improved, while reaching product titers relevant for applications remains a challenge.
Unveiling the pathogenic mechanisms of NPR2 missense variants: insights into the genotype-associated severity in acromesomelic dysplasia and short stature
Authors: Badawi Sally , Varghese Divya Saro , Raj Anjana , John Anne , Al-Musafir Hamda S. , Al-Ghamari Ahmed J. , Alshamsi Alreem R. , Ouda Sara H. , Al-Dirbashi Ghayth , Ali Bassam R. 
Published: November 2023

Introduction: Natriuretic peptide receptor 2 (NPR2 or NPR-B) plays a central role in growth development and bone morphogenesis and therefore loss-of-function variations in NPR2 gene have been reported to cause Acromesomelic Dysplasia, Maroteaux type 1 and short stature. While several hypotheses have been proposed to underlie the pathogenic mechanisms responsible for these conditions, the exact mechanisms, and functional characteristics of many of those variants and their correlations with the clinical manifestations have not been fully established.Methods: In this study, we examined eight NPR2 genetic missense variants (p.Leu51Pro, p.Gly123Val, p.Leu314Arg, p.Arg318Gly, p.Arg388Gln, p.Arg495Cys, p.Arg557His, and p.Arg932Cys) Acromesomelic Dysplasia, Maroteaux type 1 and short stature located on diverse domains and broadly classified as variants of uncertain significance. The evaluated variants are either reported in patients with acromesomelic dysplasia in the homozygous state or short stature in the heterozygous state. Our investigation included the evaluation of their expression, subcellular trafficking and localization, N-glycosylation profiles, and cyclic guanosine monophosphate (cGMP) production activity.Results and Discussion: Our results indicate that variants p.Leu51Pro, p.Gly123Val, p.Leu314Arg, p.Arg388Gln have defective cellular trafficking, being sequestered within the endoplasmic reticulum (ER), and consequently impaired cGMP production ability. Conversely, variants p.Arg318Gly, p.Arg495Cys, and p.Arg557His seem to display a non-statistically significant behavior that is slightly comparable to WT-NPR2. On the other hand, p.Arg932Cys which is located within the guanylyl cyclase active site displayed normal cellular trafficking profile albeit with defective cGMP. Collectively, our data highlights the genotype-phenotype relationship that might be responsible for the milder symptoms observed in short stature compared to acromesomelic dysplasia. This study enhances our understanding of the functional consequences of several NPR2 variants, shedding light on their mechanisms and roles in related genetic disorders which might also help in their pathogenicity re-classification.
Melatonin mitigates oxidative damage induced by anthracycline: a systematic-review and meta-analysis of murine models
Authors: Faggiano Andrea , Gherbesi Elisa , Avagimyan Ashot , Ruscica Massimiliano , Donisi Luca , Fedele Maria Antonia , Cipolla Carlo Maria , Vicenzi Marco , Carugo Stefano , Cardinale Daniela 
Published: November 2023

BackgroundOxidative stress induced by the excessive production of reactive oxygen species is one of the primary mechanisms implicated in anthracycline (ANT)-induced cardiotoxicity. There is a strong clinical need for a molecule capable of effectively preventing and reducing the oxidative damage caused by ANT. In vitro and in vivo studies conducted in mice have shown that melatonin stimulates the expression of antioxidative agents and reduces lipid peroxidation induced by ANT.MethodsWe investigated this issue through a meta-analysis of murine model studies. The outcome of the meta-analysis was to compare oxidative damage, estimated by products of lipid peroxidation (MDA = Malondialdehyde) and markers of oxidative stress (SOD = Superoxide Dismutase, GSH = Glutathione), along with a marker of cardiac damage (CK-MB = creatine kinase–myocardial band), assessed by measurements in heart and/or blood samples in mice undergoing ANT chemotherapy and assuming melatonin vs. controls. The PubMed, OVID-MEDLINE and Cochrane library databases were analysed to search English-language review papers published from the inception up to August 1st, 2023. Studies were identified by using Me-SH terms and crossing the following terms: “melatonin”, “oxidative stress”, “lipid peroxidation”, “anthracycline”, “cardiotoxicity”.ResultsThe metanalysis included 153 mice administered melatonin before, during or immediately after ANT and 153 controls from 13 studies. Compared with controls, the levels of all oxidative stress markers were significantly better in the pooled melatonin group, with standardized mean differences (SMD) for MDA, GSH and SOD being −8.03 ± 1.2 (CI: −10.43/−5.64, p &lt; 0.001), 7.95 ± 1.8 (CI: 4.41/11.5, p &lt; 0.001) and 3.94 ± 1.6 (CI: 0.77/7.12, p = 0.015) respectively. Similarly, compared with controls, CK-MB levels reflecting myocardial damage were significantly lower in the pooled melatonin group, with an SMD of −4.90 ± 0.5 (CI: −5.82/−3.98, p &lt; 0.001).ConclusionMelatonin mitigates the oxidative damage induced by ANT in mouse model. High-quality human clinical studies are needed to further evaluate the use of melatonin as a preventative/treatment strategy for ANT-induced cardiotoxicity.
Amplifying similarity to promote college STEM instructor–student mentoring relationship quality: a cluster randomized trial
Authors: Du Wenyi , Lee Hyewon , Broderick Nicole A. , Aldana Cristian Cervantes , Estrada Mica , Handelsman Jo , Maldonado Natalia , Miller Sarah , Patterson Megan S. , Sandoval Perla , Hernandez Paul R. 
Published: November 2023

IntroductionDespite numerous (co)curricular efforts, diversifying the Science, Technology, Engineering, and Mathematics (STEM) research workforce remains challenging and large segments of the U.S. population continue to be underrepresented. Promoting instructor–student mentoring relationship quality is a potentially important mechanism to support biomedical workforce diversity, as relationship quality has been positively associated with learning and persistence. We tested the impact of a “Creating Birds of a Feather” (CBoaF) intervention designed to promote perceptions of shared similarities (psychological similarity), which in turn should promote instructor-student mentoring relationship quality.MethodsThis pretest-posttest cluster randomized controlled trial was conducted with a large and diverse sample of instructors (J = 15) and the undergraduates (N = 567) enrolled in biological course-based undergraduate research experience courses at 13 universities across the U.S.ResultsMultilevel modeling results indicated that the intervention effect on undergraduates’ perceptions of psychological similarity was moderated by pretest psychological similarity. That is, among classes with low levels of similarity at pretest, the intervention group developed stronger perceptions of posttest psychological similarity than the control group, but there were no between group differences in classes with high levels of similarity at pretest. Furthermore, the intervention exhibited a positive indirect effect on posttest instructor–student mentoring relationship quality through posttest psychological similarity.DiscussionThese findings highlight the potential of the CBoaF intervention to enhance undergraduate perceptions of instructor-student psychological similarity, subsequently leading to improved instructor-student mentoring relationship quality. These insights have significant implications for initiatives that aim to promote diversity and inclusion in the STEM research workforce by emphasizing the cultivation of psychological similarity between students and instructors.
Association of periodontitis and tooth loss with extent of coronary atherosclerosis in patients with type 2 diabetes mellitus
Authors: Shen Minhua , Li Zhen , Li Huizhi , Yan Xinfeng , Feng Bo , Xu Lei 
Published: November 2023

AimThe objective was to investigate the association of periodontitis and tooth loss with extent of diabetic coronary atherosclerosis.Materials and methods272 patients who were hospitalized at Shanghai East hospital and underwent a coronary artery calcium (CAC) CT scan were enrolled in this study. Individuals were grouped based on their CAC scores into a normal-to-mild coronary atherosclerosis (AS) group (0 ≤ score ≤ 100, n=184) and a moderate-to-severe group (score≥101, n=88). Periodontitis parameters and number of missing teeth were evaluated for every patient. The severity of periodontitis was categorized as mild, moderate, or severe. The taxonomic composition of the microbiota was determined using full-length 16S ribosomal RNA gene sequencing. Salivary inflammatory factors were tested by ELISA.ResultsClinical attachment loss (CAL) (P =0.05) and the number of teeth lost (P = 0.016) were significantly higher in the moderate-to-severe coronary AS group, with these differences being more obvious in younger patients and patients with short-duration diabetes. Multivariate logistic regression analysis revealed that CAL (OR = 1.231, 95% CI = 1.066–1.214, P = 0.047) and having 10–19 missing teeth (OR = 1.604, 95% CI = 1.393–6.555, P = 0.05) were strongly associated with the presence of moderate-to-severe coronary AS. Salivary IL-6 and TNF-α levels, as well as levels of Porphyromonas gingivalis and Neisseria mucosa, were significantly elevated in the moderate-to-severe coronary AS group.ConclusionIt was found that both tooth loss and CAL were related to the extent of diabetic coronary AS. Saliva inflammatory factors and oral bacteremia may be new biomarkers for moderate-to-severe coronary AS.
The relationship between weight-adjusted-waist index and total bone mineral density in adults aged 20-59
Authors: Guo Meiqian , Lei Yi , Liu Xueqing , Li Xiang , Xu Yong , Zheng Donghui 
Published: November 2023

IntroductionAccording to reports, obesity has a significant impact on bone health. And the weight-adjusted-waist index (WWI), superior to BMI and waist circumference (WC), is a new obesity indicator arising in recent years. This research investigated the relationship between WWI and total bone mineral density (BMD) for adults aged 20 to 59.MethodsUsing data from the 2011–2018 NHANES, we looked into the independent link between WWI and total BMD as well as its nonlinearity using weighted multiple linear regression and smooth curve fitting. Two-stage linear regression models were employed to calculate the threshold effects. There were additional subgroup analyses and testing for interactions.ResultsMultiple linear regression studies on a total of 10,372 individuals showed a significant inverse link between WWI and total BMD in adults between 20 and 59 [β = -0.04, 95% CI: (-0.04, -0.03), P&lt;0.0001]. And smoking, race, and chronic kidney disease (CKD) had no significant effect on this negative connection (P for interaction &gt;0.05). In addition, we found a nonlinear relationship between WWI and total BMD in diabetic and CKD patients, for which the saturation point was 11.38 cm/√kg in the CKD patient group and 10.29 cm/√kg in the diabetic patient group.ConclusionOur analysis demonstrated a significant inverse association between WWI and total BMD in adults aged 20-59.
A content analysis of media coverage on road safety and road traffic crashes in Colombia
Authors: Saporito Kristi , Rothenstein-Henry Rachel , Zeynalova Elizaveta , Negi Nalin Singh , Mullin Sandra , Morozova Irina , Murukutla Nandita 
Published: November 2023

Introduction: In Colombia, road traffic crashes are the eighth leading cause of death. In 2017, as part of the Bloomberg Philanthropies Initiative for Global Road Safety (BIGRS), Vital Strategies supported government-led journalist trainings in Colombia to shift media discourse of road safety as a public health and development issue with known risk factors and achievable solutions, to ultimately catalyze public and policymaker concern and action. This study evaluates the effects these trainings had on road safety reporting.Methods: Articles about BIGRS road safety work published between 1 May 2017 and Aug. 30, 2021 were collected from a database maintained by communication officers. The sample included 870 articles, which were systematically analyzed for year-wise frequency by independent coders. Inter-reliability (Cohen’s Kapp K &gt; 0.94) was established using a codebook developed to identify examples of best practices shared during trainings.Results: From 2017 to 2021, there was a 27% increase in articles that situated road traffic collisions (RTCs) as due to systemic issues (thematic framing) rather than isolated events (episodic framing). Almost all observed articles used at least one WHO-recommended story angle (96%) and key element (95%). Reporting angles focused on the human story were largely underutilized (2%–5%). Government representatives (81%), mostly from the Secretary of Mobility (67%), were the most cited sources and road safety advocates were the least (3%). Use of terms “crash” and “collision” increased across the study period (crash: 47% in 2017 to 59% in 2021; collision: 0.4% in 2019 to 5% in 2020). However, RTCs continued to be widely called “accidents” (46%). More than half of articles referenced either “victims” or “vulnerable road users” (55%); use of “person” to refer to victims/vulnerable road users increased from 33% in 2017 to 56% in 2020.Conclusion: Over the course of the BIGRS journalist training program, reporting in Colombia increasingly used best practices to frame road safety as a public health issue. This highlights how media engagement is important to comprehensive road safety strategies and should be more widely adopted. Future training efforts should focus on finding the human story, and on changing overreliance on terms like “accident” that make RTCs seem inevitable.