bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial diversity and prediction function profiling of microbial communities in rose jam
Authors: Liu Ling‐Xiao , Xia Ao‐Nan , Tang Xiao‐Juan , Zhang Yan‐Zhen , Meng Xian‐Shui , Lei Sheng‐Ming , Wang Bin , Peng Shan‐Li , Liu Yun‐Guo 
Published: January 2024

AbstractThe microbial diversity of rose jam was analyzed by high‐throughput sequencing, along with functional prediction of the bacterial community. The results indicate that Pseudomonas, Pantoea, and Burkholderia emerged were the top three dominant bacterial groups. Proteobacteria was particularly abundant in R4 (99.1%) and R6 (96.12%). Zygosaccharomyces, unclassified fungi, and Botrytis constituted the top three fungal groups. The presence of unclassified OTUs was observed in all samples, particularly in R6 (52.36%), R8 (45.28), and R9 samples (39.57%). Gene prediction using PICRUSt revealed the existence of multiple KEGG functional modules associated with human metabolism in each rose jam sample. The presence of a high abundance of functional genes indicated the microbial community's diverse wide range of microgenetic resources that can be further explored for research purposes. The microbial community found in rose jam exhibits remarkable diversity and encompasses valuable functional information relevant to human health.
The Vaca Muerta mesosiderite: The path under which Fe‐Ni alloy ±C phases could have formed
Authors: Varela M. E. , Hwang S.‐L. , Shen P. , Garcia L. N. , Saavedra M. , Maruoka T. , Bose M. 
Published: January 2024

AbstractThe combined SEM and TEM studies on the metal (Fe‐Ni alloy ±C) portion of the Vaca Muerta mesosiderite reveal structural and compositional evidence at micro to nanoscale, which sheds light on the solidification of taenite + graphite as a cement, and later solid‐state precipitation process for the kamacite formation as grain boundary allotriomorph. Besides, it is proposed that the graphite veinlets formed through a complex partial melting process followed by a solidification toward the final eutectic transient coupled with the solid‐state precipitation and later decomposition ordering of taenite. The presence of defects and taenite in graphite signal formation in a liquid environment. The δ13C values of graphite in the graphite‐rich areas (e.g., ranging from −0.8 ± 1.7‰ to +15.3 ± 2.5‰) suggest a short‐circuit diffusion path for C isotope fractionation.
Functional trait correlation network and proteomic analysis reveal multifactorial adaptation mechanisms to a climatic gradient associated with high altitude in the Himalayan region
Authors: Kumari Manglesh , Kumar Rajiv 
Published: January 2024

AbstractGlobally occurring changes in environmental conditions necessitate extending our knowledge of the system‐level mechanisms underlying plant adaptation to multifactorial stress conditions or stress combinations. This is crucial for designing new strategies to maintain plant performance under simultaneous abiotic pressure. Here, we conducted our study at Rohtang Pass and sampled Picrorhiza kurroa leaves along high‐altitude gradient (3400, 3800 and 4100 meters above sea level) in the western Himalayas. The results showed the functional traits associated with morpho‐anatomical structures and eco‐physiological performances are highly variable. The air temperature and relative humidity represent dominant environmental factors among others that significantly regulate plant's physiological performance by adjusting the functional traits in altitude‐specific manner. A trait coordination network is developed among significantly altered plant functional traits, which reveals high‐altitude associated trait‐based adaptation. Moreover, it reveals leaf area shows the highest degree, while photochemical quenching reflects the weighted degree of centrality in the network. Proteomic analysis reveals various stress‐responsive proteins, including antioxidants were accumulated to deal with combined stress factors. Furthermore, a high‐altitudinal protein interaction network unravels key players of alpine plant adaptation processes. Altogether, these systems demonstrate a complex molecular interaction web extending the current knowledge of high‐altitudinal alpine plant adaptation, particularly in an endangered medicinal herb, P. kurroa.
Transcriptional profiling identifies the early responses to Puccinia triticina infection in the adult plant leaf rust resistant wheat variety Toropi
Authors: Casassola Alice , Ereful Nelzo C. , Zanella Camila M. , Pandey Pooja , Brammer Sandra P. , Chaves Márcia S. , Martinelli José A. , Boyd Lesley A. 
Published: January 2024

AbstractLeaf rust, caused by Puccinia triticina (Pt), is a major disease of wheat and a significant problem for wheat production in Brazil. The Brazilian variety Toropi, released in 1965, has maintained high levels of field, adult plant resistance (APR) to leaf rust across global locations, while microscopic studies have indicated prehaustorial resistance mechanisms. Analyses of gene expression in flag leaves of Toropi, during the early stages of Pt infection, were undertaken to explore the mechanisms behind the APR in Toropi. Differential expression of wheat genes was undertaken, comparing Pt‐ to mock‐inoculated and Pt‐ to Pt‐inoculated time points. Analysis of gene expression indicated a strong response to Pt, which was fully active by 6 h after inoculation (hai). More genes were downregulated than upregulated, particularly at 6 and 12 hai. Gene Ontology enrichment analysis indicated a shutting down of RNA and protein synthesis and an early effect on photosynthesis, with disruption of the electron transfer chain. Analyses of upregulated genes identified genes involved in ATP‐binding and protein kinase activity at 6 hai, supporting a rapid metabolic response to Pt infection. A general upregulation of genes involved in transport and metabolism indicated the need to relocate protein and organic‐based resources. Alignment of differentially expressed genes with the genomic regions defining four leaf rust APR quantitative trait loci (QTLs) in Toropi identified candidate resistance genes, including a sugar transporter, a receptor kinase and a seven‐transmembrane MLO family protein. In addition, 60 Pt genes were identified, 11 being annotated as potential effector proteins.
The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway
Authors: Zhao Meiqi , Lei Yijing , Wu Ling , Qi Haoran , Song Zihe , Xu Meng 
Published: January 2024

SUMMARYAs sessile organisms, plants experience variable environments and encounter diverse stresses during their growth and development. Adventitious rooting, orchestrated by multiple coordinated signaling pathways, represents an adaptive strategy evolved by plants to adapt to cope with changing environmental conditions. This study uncovered the role of the miR159a‐PeMYB33 module in the formation of adventitious roots (ARs) synergistically with abscisic acid (ABA) signaling in poplar. Overexpression of miR159a increased the number of ARs and plant height while reducing sensitivity to ABA in transgenic plants. In contrast, inhibition of miR159a (using Short Tandem Target Mimic) or overexpression of PeMYB33 decreased the number of ARs in transgenic plants. Additionally, miR159a targets and cleaves transcripts of PeMYB33 using degradome analysis, which was further confirmed by a transient expression experiment of poplar protoplast. We show the miR159a‐PeMYB33 module controls ARs development in poplar through ABA signaling. In particular, we demonstrated that miR159a promotes the expression of genes in the ABA signaling pathway. The findings from this study shed light on the intricate regulatory mechanisms governing the development of ARs in poplar plants. The miR159a‐PeMYB33 module, in conjunction with ABA signaling, plays a crucial role in modulating AR formation and subsequent plant growth.
A Systems Approach to Biomechanics, Mechanobiology, and Biotransport
Authors: Peirce-Cottler Shayn M. , Sander Edward A. , Fisher Matthew B. , Deymier Alix C. , LaDisa John F. , O'Connell Grace , Corr David T. , Han Bumsoo , Singh Anita , Wilson Sara E. , Lai Victor K. , Clyne Alisa Morss 
Published: January 2024

Abstract
The human body represents a collection of interacting systems that range in scale from nanometers to meters. Investigations from a systems perspective focus on how the parts work together to enact changes across spatial scales, and further our understanding of how systems function and fail. Here, we highlight systems approaches presented at the 2022 Summer Biomechanics, Bio-engineering, and Biotransport Conference in the areas of solid mechanics; fluid mechanics; tissue and cellular engineering; biotransport; and design, dynamics, and rehabilitation; and biomechanics education. Systems approaches are yielding new insights into human biology by leveraging state-of-the-art tools, which could ultimately lead to more informed design of therapies and medical devices for preventing and treating disease as well as rehabilitating patients using strategies that are uniquely optimized for each patient. Educational approaches can also be designed to foster a foundation of systems-level thinking.
Experimental and Numerical Investigation of Effect of Inclination on sCO2 Heat Transfer in a Circular Pipe
Authors: Gabriel-Ohanu Emmanuel , Shah Alok , Khadse Akshay , Fernandez Erik , Kapat Jayanta S. 
Published: January 2024

Abstract
Supercritical carbon dioxide (sCO2) can be utilized as a working fluid in various thermal systems including large-scale power cycles; portable power production units, centralized coolant systems, and standalone cooling devices. However, the lack of accurate prediction tools such as heat transfer coefficient correlations and insufficient research studies about the mechanisms controlling heat transfer processes are hindering its practical realization for key energy and cooling systems. The overall objective of this study is to extend fundamental knowledge about heat transfer and fluid dynamic processes in conduits pertinent to sCO2 with an emphasis on flow direction and inclination effects. This paper presents the study on effects of gravity, buoyancy on sCO2 flow at temperatures near and away from the pseudo-critical temperature. The experimental setup consists of a high temperature and pressure sCO2 heat transfer loop and flow testing facility. Recently, researched sCO2 heat exchangers can have tubes oriented at different angles such as 45 deg or 90 deg to horizontal. For optimized design of efficient and cost-effective turbomachinery components utilizing sCO2 as the heat transfer fluid, an understanding of convective heat transfer inside a tube/pipe is equally as important as external heat transfer. This paper presents an experimental and numerical study on sCO2 heat transfer at various inclinations with angles ranging from 0 deg (horizontal) to 90 deg (vertical) along with upward and downward flow direction with different inlet temperatures. Thermocouple-based temperature measurement is utilized at multiple locations within the test section axially and circumferentially to study the temperature distributions on the tube surface. Computational fluid dynamics (CFD) simulations have been performed using ANSYS Fluent to complement experimental data. The CFD and experiment have been analyzed against the well-known Gnielinski Nusselt number correlation.
Rapid development of spiral garnets during subduction zone metamorphism revealed from high-resolution Sm-Nd garnet geochronology
Authors: Farrell Thomas P. , Aerden Domingo , Baxter Ethan F. , Starr Paul G. , Williams Mike L. 
Published: January 2024

Abstract
Multiple studies have applied zoned garnet geochronology to place temporal constraints on the rates of metamorphism and deformation during orogenesis. We report new high-resolution isotope dilution–thermal ionization mass spectrometry Sm-Nd isochron ages on concentric growth zones from microstructurally and thermodynamically characterized garnets from the Betic Cordillera, southern Spain. Our ages for the garnet core (13.64 ± 0.31 Ma), mantle (13.41 ± 0.37 Ma), and rim (13.34 ± 0.45 Ma) indicate rapid garnet growth and are consistent with published garnet ages interpreted to reflect high-pressure metamorphism in the region. Thermodynamic analysis indicates garnets grew during subduction at ~1.5–2.0 GPa and 570–600 °C. The core to rim duration of spiral garnet growth was just a few hundred thousand years. While other zoned garnet studies have shown similar rapid growth in subduction zone settings, this is the first documentation of such rapid growth of a spiral garnet. Combining this garnet growth duration with the magnitude of spiral inclusion trail curvature, we compute a strain rate of ~10−13 s−1, an order of magnitude faster than all previous spiral garnet studies. We interpret that these spiral garnets recorded a rapid pulse of deformation and strain during the final stages of subduction and incipient exhumation.
Newborn face mask ventilation training using a standardised intervention and respiratory function monitor: a before and after manikin study
Authors: Ni Chathasaigh Caitriona M , Smiles Linda , Curley Anna E , O'Currain Eoin 
Published: January 2024

ObjectiveThe International Liaison Committee on Resuscitation has recommended improvements in training for neonatal resuscitation, highlighting the potential role of respiratory function monitors (RFMs). Our objective was to determine whether a manikin-based, standardised face mask ventilation training intervention using an RFM with a simple visual display reduced face mask leak.DesignMulticentre, before and after study. Participants and instructors were blinded to the RFM display during both assessment periods.ParticipantsHealthcare professionals working or training in a hospital providing maternity and neonatal services.InterventionAll participants underwent a training intervention on positive pressure ventilation using a modified, leak-free manikin and RFM. The intervention consisted of a demonstration of optimal face mask ventilation technique, training in RFM interpretation with corrective strategies for common scenarios and a period of deliberate practice. Each participant performed 30 s of positive pressure ventilation blinded to the RFM display before and after training.Main outcome measuresThe primary outcome was face mask leak (%) measured after training. Secondary outcome measures included expired tidal volume, inflating pressures and ventilation rate. Adjustments made to technique during training were an important qualitative outcome.ResultsFour hundred and fourteen participants were recruited over a 13-month period from April 2022, and 412 underwent analysis. Median (IQR) face mask leak before training was 31% (10–69%) compared with 10% (6–18%) after training (p<0.0001). Improvements were noted across all other ventilation parameters.ConclusionStandardised face mask ventilation training using an RFM with simple visual feedback led to a significant reduction in leak.
Strategies to support maternal and early childhood wellness: insight from parent and provider qualitative interviews during the COVID-19 pandemic
Authors: Simpson Andrea N , Baxter Nancy N , Sorvari Anne , Boury Himani , Shore Eliane M , Bogler Tali , Campbell Douglas , Gagliardi Anna R 
Published: January 2024

ObjectivesThe COVID-19 pandemic resulted in rapid changes to the delivery of maternal and newborn care. Our aim was to gain an understanding from parents and healthcare professionals (HCPs) of how the pandemic and associated public health restrictions impacted the peripartum and postpartum experience, as well as longer-term health and well-being of families.DesignQualitative study through focus groups.SettingOntario, Canada.ParticipantsHCPs and parents who had a child born during the COVID-19 pandemic.InterventionsSemistructured interview guide, with questions focused on how the pandemic impacted their care/their ability to provide care, and strategies to improve care and support now or in future situations with similar healthcare restrictions.Outcome measuresThematic analysis was used to describe participant experiences and recommendations.ResultsWe included 11 HCPs and 15 parents in 6 focus groups. Participants described their experiences as ‘traumatic’, with difficulties in accessing prenatal and postpartum services, and feelings of distress and isolation. They also noted delays in speech and development in children born during the pandemic. Key recommendations included the provision of partner accompaniment throughout the course of care, expansion of available services for young families (particularly postpartum), and special considerations for marginalised groups, including access to technology for virtual care or the option of in-person visits.ConclusionsOur findings may inform the development of healthcare system and organisational policies to ensure the provision of maternal and newborn care in the event of future public health emergencies. Of primary importance to the participants was the accommodation of antenatal, intrapartum and postpartum partner accompaniment, and the provision of postpartum services.
Robotic beyond total mesorectal excision (TME) for locally advanced or recurrent rectal cancer: a systematic review protocol
Authors: Panagiotopoulou Ioanna Georgiou , Przedlacka Anna , Piozzi Guglielmo Niccolò , Mills Graham A , Harper Mick , Khan Jim S 
Published: January 2024

IntroductionThe surgical treatment for locally advanced or recurrent rectal cancer requires oncological clearance with a pelvic exenteration or a beyond total mesorectal excision (TME). The aim of this systematic review is to explore the safety and feasibility of robotic surgery in locally advanced and recurrent rectal cancer by evaluating perioperative outcomes, oncological clearance rates, and survival and recurrence rates postrobotic beyond TME surgery.MethodsThe systematic review will include studies published until the end of December 2023. The MEDLINE, EMBASE and Scopus databases will be searched. The screening process, study selection, data extraction, quality assessment and analysis will be performed by two independent reviewers. Discrepancies will be resolved by consensus with a third independent reviewer. The risk of bias will be assessed with validated scores. The primary outcomes will be oncological clearance, overall and disease-free survival, and local and systemic recurrence rates post robotic or robot-assisted beyond TME surgery for locally advanced or recurrent rectal cancer. Secondary outcomes will include perioperative outcomes.Ethics and disseminationNo ethical approval is required for this systematic review as no individual patient cases are studied requiring access to individual medical records. The results of the systematic review will be disseminated with conference presentations and peer-reviewed paper publications.PROSPERO registration of the studyCRD42023408098.
Risk of suicide in patients with thyroid cancer: protocol for a systematic review and meta-analysis
Authors: Li Chen , Zhang Mengmeng , Wang Quan , Jiang Kewei , Ye Yingjiang 
Published: January 2024

IntroductionIn recent years, the incidence of thyroid cancer has increased manyfold and young adults, who have a greater financial burden and occupational stress, comprise a large number. Previous studies have shown mixed results, even distinct results, on suicide rates among thyroid cancer survivors. As the overdiagnosis and overtreatment of thyroid cancer has gradually become a topical issue, the study aims to summarise the risk of suicide among patients with thyroid cancer to provide robust evidence of the effects of thyroid cancer on suicide.Methods and analysisA total of six databases (MEDLINE, Embase, Web of Science Core Collection, PsycINFO, CINAHL and Google Scholar) will be searched according to MeSH, subheadings, and free words, and the planned search date is 31 Jnauary 2024. The search strategy had three parts, such as suicide, cancer and epidemiological studies, moreover, we will collect the detailed suicide information by reviewers’ extraction. Standard mortality ratio (SMR) was used as the outcome measure, when SMRs were not available, the risk ratio, HR and detailed number of suicides were extracted to calculate the SMRs.Ethics and disseminationThe Institutional Review Board of Peking University People’s Hospital provided ethical approval exemption and approved the data collection and subsequent analyses in accordance with the Declaration of Helsinki as revised in 2013.PROSPERO registration numberCRD42023445542.
Effects of ferric derisomaltose on postoperative anaemia in adult spinal deformity surgery: a study protocol for a randomised controlled trial
Authors: Zhan Jing , Jiao Yang , Chen Weiyun , Huang Yuguang , Shen Jianxiong 
Published: January 2024

IntroductionPostoperative anaemia is prevalent in adult spinal deformity (ASD) surgery in association with unfavourable outcomes. Ferric derisomaltose, a novel iron supplement, offers a promising solution in rapidly treating postoperative anaemia. However, the clinical evidence of its effect on patients receiving spinal surgery remains inadequate. This randomised controlled trial aims to evaluate the safety and efficacy of ferric derisomaltose on postoperative anaemia in ASD patients.Methods and analysisThis single-centre, phase 4, randomised controlled trial will be conducted at Department of Orthopaedics at Peking Union Medical College Hospital and aims to recruit adult patients who received ASD surgery with postoperative anaemia. Eligible participants will be randomly assigned to receive ferric derisomaltose infusion or oral ferrous succinate. The primary outcome is the change in haemoglobin concentrations from postoperative days 1–14. Secondary outcomes include changes in iron parameters, reticulocyte parameters, postoperative complications, allogeneic red blood cell infusion rates, length of hospital stay, functional assessment and quality-of-life evaluation.Ethics and disseminationThis study has been approved by the Research Ethics Committee of Peking Union Medical College Hospital and registered at ClinicalTrials.gov. Informed consent will be obtained from all participants prior to enrolment and the study will be conducted in accordance with the principles of the Declaration of Helsinki. The results of this study are expected to be disseminated through peer-reviewed journals and academic conferences.Trial registration numberNCT05714007.