bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments
Authors: Hashemi Seyedeh Zahra , Fooladi Jamshid , Vahidinasab Maliheh , Hubel Philipp , Pfannstiel Jens , Pillai Evelina , Hrenn Holger , Hausmann Rudolf , Lilge Lars 
Published: January 2024

Background: Due to their structural features, biosurfactants reveal promising physicochemical properties, making them interesting for various applications in different fields, such as the food, cosmetics, agriculture, and bioremediation sectors. In particular, the bioproduction of surfactin, one of the most potent microbially synthesized biosurfactant molecules, is of great interest. However, since the wild-type productivities are comparably low, stimulatory environmental conditions have to be identified for improved bioproduction This study aims to find a correlation between the hydrophobicity and production of the biosurfactant surfactin by B. subtilis isolates from crude-oil-contaminated soil and water. Methods: The surfactin production yield was characterized in adapted batch cultivations using high-performance thin-layer liquid chromatography (HPTLC). Defined hydrophobic environmental conditions were achieved by supplementation with hexadecane or polystyrene beads, and the effects on biosurfactant production were measured. Adaptations at the protein level were analyzed using mass spectrometry measurements. Results: The correlation between hydrophobicity and surfactin production was characterized using Bacillus subtilis strains ZH1 and P7 isolated from crude-oil-contaminated soil and water. Since these isolates show the biodegradation of crude oil and hexadecane as hydrophobic substrates, respectively, a first-time approach, using polystyrene beads, was applied to provide a hydrophobic environment. Interestingly, contrary to popular opinion, reduced biosurfactant production was determined. Using mass spectrometric approaches, the physiological effects of co-cultivation and the cellular response at the protein level were investigated, resulting in altered quantities of stress proteins and proteins involved in the carbon metabolism counter to polystyrene beads. Conclusions: Contrary to common opinion, increasing hydrophobicity does not have a stimulating effect, and even reduces the effect on the bioproduction of surfactin as the main biosurfactant using selected B. subtilis strains.
Optimization of the treatment of caries permanent teeth with different degree of enamel resistance beside children with innate unjoining lip and palate
Authors: Ashurov G. G. , Obidov S. A. , Karimov S. M. 
Published: January 2024

Aim. To select the optimal method for caries treatment of permanent teeth with different degree of enamel cariesresistance in children with innate unjoining lip and palate. Materials and methods. Beside 40 children was examined the condition of hard tissue of teeth. In dependent of enamel resistance was using atraumatic restorative treatment with using of new technology of prepared teeth by minimum invasive method with following filling by glassionomer cement. Among examined children, using enamel resistance test, revealed four level of resistance. Depending on the enamel resistance level, corresponding treatment was provided: a child with high and moderate enamel resistance was organized traditional treatment of caries. Children with low and very low enamel resistance had a complex of treatment-preventive approach in 3 directions: atraumatic restorative treatment of caries by method of minimal invasive prepared; local pathogenetic remotherapy by deep fluoridation combined with professional oral hygiene lessons, followed by filling of glassionomer’s cement; general pathogenetic therapy (in conjunction with the internist oral administration of calcium preparations, vitamin complexes and rational nutrition). Results. Complex of integration approach treatment of caries in children with innate unjoining lip and palate was highly reliable (р<0.001) effective in depending of source level enamel resistance. This approach helped prevent the development of secondary caries around early assessed filling material. Conclusions. Beside children with innate unjoining lip and palate in the course of active realization of the complex method of the treatment tooth decay was noted reliable reduction of cariesology complications after filling of carious cavity under low and very low enamel resistance on all under study parameters.
Regeneration of the apical region of the tooth after the treatment of destructive periodontitis with cement resorption
Authors: Glinkin V. V. , Voronov I. A. 
Published: January 2024

Aim. Destructive changes in the tissues of the tooth and periodontium entail resorption of the hard tissues of the tooth in the apical region. The goal is to identify morphological changes in root tissues with destructive periodontitis and resorption phenomena after the treatment of the tooth with calcium-containing preparations using a scanning electron microscope. Materials and methods. Examination of teeth treated for destructive forms of periodontitis with apical resorption was performed using a JSM-6490LV scanning electron microscope (JEOL, Japan) with an energy-dispersive attachment INCA Penta FETx3 (OXFORD Instruments, England). Results. In the apical part of the root there is a cellular mixed layered cementum. The dentin and the dentin-cementum junction are highly calcified. On top of them is cellular cement with lacunae of cementoblasts. The relief of the zone of cell-free cement has a wavy structure, consists of dense collagen fibers with a high content of calcium. With external root resorption, we observe extensive destruction of the cementoblast layer. An area of newly formed cementum was found inside the apical foramen. On the apical surface, where the resorption covered the dentin layer, there was an area of newly formed cementum. Its cellular layer consisted of round-shaped cells rising above the tissue of a homogeneous structure, smoothly flowing into the spongy tissue denting the bone. Discussion. It can be assumed that prolonged use of calcium-containing drugs leads to calcification. The presence of dense connective tissue in the periodontium indicates regenerative processes. The control of cement formation remains uncertain. Conclusions. Perhaps we are seeing a reparative atypical regeneration of the cement of the apical part of the tooth root. Dentinal calcification. In the lateral parts of the apical region, there is an outer layer of cementum with no cementoblasts. An increase in the thickness of the cement was noted. It can be assumed that we observe the phenomenon of osteogenesis in the area of strong resorption.
Current Aspects of Regenerative Endodontics: A Systematic Review
Authors: Mitronin A. V. , Archakov K. A. , Ostanina D. A. , Mitronin Yu. A. , Khizrieva T. V. 
Published: January 2024

Relevance. The modern concept of endodontic treatment in most clinical cases involves extirpation of the pulp with the loss of its plastic, trophic, sensory, and protective functions. In this regard, the search for ways to recreate lost cells and the main substance of the pulp and periodontium to replace the intraradicular space with “vital” tissue. Regenerative endodontics based on the principles of tissue engineering and offers techniques for restoring damaged tissues of the pulp-dentin complex based on the use of stem cells, growth factors, and an organic matrix. Aim. Systematize information presented in scientific articles about research conducted, opportunities and development prospects in the field of regenerative endodontics. Materials and methods. A search of literature sources was carried out in the PubMed, dissercat.com, elibrary database.ru, cyberleninka.ru, by keywords, “regenerative endodontics”, “stem cells”, “growth factors”, “pulp-dentin complex” with a choice of article types “Clinical Trial”, “Meta-Analysis”, “Review”, "Systematic Review". Results. After analyzing the literature review, an idea was obtained about the current stage of development of regenerative endodontics, preclinical and clinical studies on the restoration of the pulp-dentin complex. Clinical cases based on the triad of regenerative endodontics are presented, as well as an algorithm for performing these therapeutic procedures. Conclusions. Regenerative endodontics is a rapidly developing discipline whose clinical advances are primarily related to the treatment of immature tooth roots, which results in continued root development. Subsequent scientific research is aimed at obtaining significant results in the treatment of a fully formed permanent tooth, which will ensure a unique restoration of the immunological, sensory and protective properties of the pulp-dentine complex.
Features of temporary teeth hard tissues affecting caries resistance
Authors: Bagdasarova I. V. , Kostinskaya M. V. , Miral Al. Kamme 
Published: January 2024

Aim. Features of microscopic structure of enamel and dentin of intact temporary teeth, which are the cause of their reduced caries resistance. Materials and methods. Current information in the electronic databases Google Scholar and PubMed was examined through a systematic literature review. Articles with content related to the prevalence of dental caries in temporary teeth and the relationship between caries and tooth hard tissue structure were selected and included. Results. A total of 327 publications were reviewed. After analyzing the literature according to the inclusion criteria, the final number was 52. Conclusions. Based on the analyzed data, the structure of the hard tissues of temporary teeth is a predisposing factor for the occurrence and development of dental caries. Decreased thickness of enamel and dentin, increased concentration of organic matrix in them due to incomplete mineralization, microporosity of enamel, abnormal development of dentin layers, increased number of hypomineralized and irregular areas, underdevelopment of pulp, impaired function of odontoblasts, increased adhesion of microorganisms to the enamel surface – all these features contribute to the rapid and progressive development of caries in temporary teeth.
Efficacy evaluation of using hypoallergenic toothpastes in children with a burdened allergy anamnesis
Authors: Kondratenko I. D. , Nikolskaya I. A. , Kuliev R. M. , Vostrikov V. O. , Voronetskaya V. A. , Novikova A. 
Published: January 2024

Aim. To conduct a clinical evaluation of the efficacy of using hypoallergenic children's toothpastes “R.O.C.S. PRO Baby" and "R.O.C.S. PRO Junior Creamy Pudding" in patients aged 2 to 11 years with a burdened allergy history. Materials and methods. The study included 58 children aged 2 to 11 years with a burdened allergy history. Before the start of the study, they were divided into two groups: the first included 28 children aged 2-3 years, whose parents had been brushing their teeth with « R.O.C.S. PRO Baby» hypoallergenic paste for 3 months. The second group included 30 childrenaged 6-11 years who provided oral care using the paste « R.O.C.S. PRO Junior Cream pudding ». During the control dental examinations, the determination of the possible locally irritating and allergenic effects of the studied toothpastes was carried out by visual examination of the oral mucosa of patients of both groups, as well as interviews and questionnaires of study participants and their parents. In patients of the second group, the hygienic condition of the oral cavity was assessed by determining the PHP hygiene index, PMA and SBI indices to assess the condition of periodontal tissues. Results. During the study, patients with a burdened allergy history did not have a locally irritating and allergenic effect of hypoallergenic toothpastes "R.O.C.S. PRO Baby" and "R.O.C.S. PRO Junior Cream pudding" on the oral mucosa. When using toothpastes, there was a significant decrease in the PHP, PMA and SBI indices by 58.47 %, 47.4 % and 43.2 %, respectively. Conclusions. The obtained data allow us to conclude that hypoallergenic toothpastes « R.O.C.S. PRO Baby » and « R.O.C.S. PRO Junior Creamy Pudding » meets the safety and efficacy criteria for cleaning teeth and can be recommended to patients with a history of allergies aged 2 to 11 years for daily hygienic oral care.
Antinutrients in Grain-Based Plant Drinks: Scoping Review
Authors: Strizhko Maria N. 
Published: January 2024

Introduction: Plant-based beverages in the consumer market are positioned as an alternative to natural milk, including their nutritional value. Traditional milk raw materials are characterized by high nutritional value due to the optimal balance of components and their easy digestibility. However, the plant materials used in the production of drinks contain anti-nutritional substances. Anti-nutritional nutrients can limit the bioavailability of primary nutrients, leading to impoverishment of the human diet and a decrease in the nutritional value of food products.Purpose: The purpose of this scoping review is a comprehensive analysis of various anti-nutritional factors in grain-based plant drinks with an assessment of methods and conditions for their inhibition.Materials and Methods: This scoping review was conducted in accordance with the guiding principles of PRISMA-ScR. The databases SCOPUS, ScienceDirect, Google Scholar were used for article selection. The search was carried out for the period 2017-2022. As a result of the search, 77 publications from 35 countries worldwide were selected. The subject field review protocol was drafted and registered on the Open Science Framework website (https://osf.io/gcb3y).Results: Out of 4432 selected publications, 77 met the inclusion criteria for the review. The analysis of the selected publications identified the main anti-nutritional substances present in grain drinks. These nutrients include phytic acid, phytates, lectins, saponins, oxalates, enzyme inhibitors. The authors of a significant portion of the publications (70%) devoted to the issue of antinutrients in the product, investigate this question within the technology of producing plant drinks. Trends such as negative and positive effects of antinutrients, methods of inhibiting anti-nutritional substances were identified. The obtained results allowed highlighting a new direction of non-traditional methods of inhibiting antinutrients, which had not been recorded before.Conclusion: The main area of application of the research results can be the expansion of the scientific-practical database about antinutritional substances and the practical implementation of the proposed recommendations in the production cycle. The obtained data will significantly increase the nutritional value of grain-based beverages.
Development of enriched bakery products using biotransformation in starter cultures of protein-containing vegetable additives
Authors: Kosheleva Anna I. , Bogatyreva Tatyana G. , Belyavskaya Irina G. 
Published: January 2024

Introduction: In the bakery industry, the key task is to produce quality products from diverse raw materials for healthy public nutrition. Technologies include the use of additives to enhance quality, biotransformation methods with starters, and the selection of enzymes and microorganisms. Biotransformation modifies the raw material, resulting in a product with superior taste qualities and nutritional value. The use of starters requires careful selection of microorganisms to optimize product quality.Materials and Methods: The objects of research in the work were bakery products obtained by biotransformation of the studied plant raw materials, which was carried out by pure cultures of probiotic microorganisms - mesophilic homo- and heterofermentative lactic acid and propionic acid bacteria: Lactobacillus casei, L.plantarum, L.acidophilus, L.fermenti, L.brevis, Propionibacterium freudenreichii subsp. Shermanii. The work uses generally accepted standard methods for determining organoleptic and physico-chemical quality indicators of semi-finished products and finished bakery products. The indicator of resistance to microbiological spoilage of the developed bakery product was determined.Results: Results: The use of defatted soy flour and sunflower protein as sources of plant protein has been justified. Based on comprehensive experimental studies, a rational method was identified for introducing defatted soy flour into the starter at 25% of the flour mass, which involves acid and enzymatic hydrolysis followed by biotransformation with a bacterial starter based on a composition of lactic acid microorganisms. Additionally, to increase the content of protein balanced in amino acid composition, as well as macro and microelements, vitamins, and antioxidants, a dosage of sunflower protein was selected at 10% of the flour mass in the product recipe.Conclusion: A technology for enriched bakery products has been developed, and the nutritional value has been calculated. A comprehensive assessment of the quality of the finished products based on organoleptic and physico-chemical indicators has been conducted, and the shelf life has been determined.
Prospects for the use of probiotic organisms to develop alternative strategies for disinfection and prevention of infectious diseases
Authors: Kishilova Svetlana Anatolievna 
Published: January 2024

Introduction: An important feature of microorganisms is the ability to adapt to adverse external influences, including the development of multiple antimicrobial resistance, leading to such negative consequences for humans as nosocomial infections, problems with cleaning rooms, medical and industrial equipment. The active use of chemical disinfectants for high-quality disinfection and cleaning has a number of disadvantages, including the risks of pathogens with new mechanisms of resistance to antimicrobial agents. The search for new, effective and safe antimicrobial agents as an alternative to chemical disinfectants is relevant. The use of probiotic strains of microorganisms, including lactic acid bacteria, in this capacity may be a promising area of research.Objective: to analyze the literature data on studies of the potential of probiotic organisms, including lactic acid bacteria, to develop alternative disinfection and prevention strategies.Materials and methods: This review of the subject field has been prepared based on the guiding principles of PRISMA-ScR. SCOPUS, Google Scholar, and RSCI databases were used. The works in Russian and English were considered, for the period 1995-2023.Results: The review included 89 articles exploring the disadvantages of traditional disinfection methods and the search for alternative sanitation strategies. The description of various solutions for the introduction of probiotic purification methods is systematized – using bacteriophages, probiotic microorganisms of R. Bacillus and representatives of lactic acid bacteria as active agents.Conclusions: A system of sanitary measures based on probiotic biodesinfectants, including lactic acid bacteria, can be included among the tools for countering pathogens, including their biofilms and forms with multidrug resistance. The introduction of a probiotic hygienic cleaning system, without having a negative impact on the environment, can increase the effectiveness of traditional hygienic preventive measures both in medical institutions and in production.
Enzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Review
Authors: Kruchinin Aleksandr Gennadievich , Bolshakova Ekaterina Ivanovna 
Published: January 2024

Introduction: For 14 years, interest in the use of dairy raw materials for the production of bioactive peptides (BPs) with antihypertensive, antioxidant and antidiabetic effects has been in a phase of rapid growth because of the need to prevent different diseases. Particular attention is being paid to enzymatic production method, as it allows for a targeted process of BP extraction with specified properties.The purpose of this scoping review was to summarize and systematize the experience of international research over the last 14 years in the field of FG as a method to extract BPs from milk protein substrates.Materials and Methods: This scoping review was performed in accordance with the PRISMA-ScR guidelines. Scientific papers were searched in the Google Scholar database, because allows searching for scientific sources without restrictions on their format and type of scientific data presentation (book, dissertation, article), as well as includes scientific sources from other databases and performs relevance sorting.Results: The search identified 72 sources published between 2007 and 2021 in English. Included sources are devoted to hydrolysis by starter bacteria enzymes (SBE) - 50% and by commercial enzyme preparations (CEP) - 50%.Conclusion: There are a number of challenges that need to be addressed in order to increase the level of commercialization of BP production through directed hydrolysis. Creation of databases; building up the research base on co-fermentation by microorganisms and commercial enzymes; conducting PCR studies of microbial protease genes will increase the practical applicability of research and the validity of conducting more evidence-based in vivo and clinical studies.
Principles of formation of modular technologies of enteral nutrition products
Authors: Agarkova Evgenia Yuryevna , Belyakova Zinaida Yuryevna , Kondratenko Vladimir Vladimirovich 
Published: January 2024

Introduction. Enteral nutrition products (EP) are represented on the market by a wide range of imported products, the technology and formulations of which are unique and are objects of intellectual property of foreign scientific centers. The application of a unified methodological approach to the design of EP products will make it possible to transfer this process from an empirical to a scientifically based plane.Purpose of research is to create a rational set of technological solutions for the production of specialized EP products based on the principles of modularity, taking into account the cascade of technological qualitative and quantitative transformations.Materials and methods. Digital matrices of patient conditions, EP products and ingredients were used as materials. The main research method was advanced digital profiling, taking into account the transformation of nutrients in the production process.Results. According to the formed matrices of the chemical composition of imported EP products, the criteria relationships of their digital profiles and state profiles were established. Based on the principles of food combinatorics and digital profiling, an algorithm for an improved approach to the design and production of EP is proposed. A generalized modular technology for the production of EP products has been developed and its variability has been proved without changing the structure due to the combinatorial versatility of each of the set of models. The degree of its available combinatorial variability has been established, which is functionally dependent on the number of combinatorially manipulated ingredients, taking into account all possible k-component compositions. The required set of formulations of EP products is a set of solutions in terms of design with the possibility of determining the vector of tasks to be solved.Conclusions. The obtained research results can be applied in the food industry in terms of unified production of specialized products.