bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of artificial intelligence-based analysis software to support screening system of cervical intraepithelial neoplasia
Authors: Ouh Yung-Taek , Kim Tae Jin , Ju Woong , Kim Sang Wun , Jeon Seob , Kim Soo-Nyung , Kim Kwang Gi , Lee Jae-Kwan 
Published: January 2024

AbstractCervical cancer, the fourth most common cancer among women worldwide, often proves fatal and stems from precursor lesions caused by high-risk human papillomavirus (HR-HPV) infection. Accurate and early diagnosis is crucial for effective treatment. Current screening methods, such as the Pap test, liquid-based cytology (LBC), visual inspection with acetic acid (VIA), and HPV DNA testing, have limitations, requiring confirmation through colposcopy. This study introduces CerviCARE AI, an artificial intelligence (AI) analysis software, to address colposcopy challenges. It automatically analyzes Tele-cervicography images, distinguishing between low-grade and high-grade lesions. In a multicenter retrospective study, CerviCARE AI achieved a remarkable sensitivity of 98% for high-risk groups (P2, P3, HSIL or higher, CIN2 or higher) and a specificity of 95.5%. These findings underscore CerviCARE AI's potential as a valuable diagnostic tool for highly accurate identification of cervical precancerous lesions. While further prospective research is needed to validate its clinical utility, this AI system holds promise for improving cervical cancer screening and lessening the burden of this deadly disease.
Identification of four latent classes of acute respiratory distress syndrome using PaO2/FIO2 ratio: an observational cohort study
Authors: Loewen Calvin , Dufault Brenden , Mooney Owen , Olafson Kendiss , Funk Duane J. 
Published: January 2024

AbstractBiological phenotypes in patients with the acute respiratory distress syndrome (ARDS) have previously been described. We hypothesized that the trajectory of PaO2/FIO2 ratio could be used to identify phenotypes of ARDS. We used a retrospective cohort analysis of an ARDS database to identify latent classes in the trajectory of PaO2/FIO2 ratio over time. We included all adult patients admitted to an intensive care unit who met the Berlin criteria for ARDS over a 4-year period in tertiary adult intensive care units in Manitoba, Canada. Baseline demographics were collected along with the daily PaO2/FIO2 ratio collected on admission and on days 1–7, 14 and 28. We used joint growth mixture modeling to test whether ARDS patients exhibit distinct phenotypes with respect to both longitudinal PaO2/FIO2 ratio and survival. The resulting latent classes were compared on several demographic variables. In our study group of 209 patients, we found that four latent trajectory classes of PaO2/FIO2 ratio was optimal. These four classes differed in their baseline PaO2/FIO2 ratio and their trajectory of improvement during the 28 days of the study. Despite similar baseline characteristics the hazard for death for the classes differed over time. This difference was largely driven by withdrawal of life sustaining therapy in one of the classes. Latent classes were identified in the trajectory of the PaO2/FIO2 ratio over time, suggesting the presence of different ARDS phenotypes. Future studies should confirm the existence of this finding and determine the cause of mortality differences between classes.
Efficacy of the insect growth regulator novaluron in the control of dengue vector mosquitoes Aedes aegypti and Ae. albopictus
Authors: Herath J. M. Manel K. , De Silva W. A. Priyanka P. , Weeraratne Thilini C. , Karunaratne S. H. P. Parakrama 
Published: January 2024

AbstractInsect Growth Regulator (IGR) novaluron is an alternative to synthetic neuro-inhibitory insecticides. Present study was designed to assess appropriate dosages of novaluron for dengue vector control. Larvae of Aedes aegypti and Ae. albopictus were exposed to a concentration series of novaluron (Rimon EC10) for two fixed exposure periods of 7-days and 14-days to determined LC50 and LC99 values. Inhibition of adult emergence (IE50 and IE99) was determined by a 14-day exposure. Semi-field experiments were conducted by exposing cohorts of Ae. aegypti larvae to IE99, 2 × IE99 and 10 × IE99 novaluron concentrations in water storage buckets (10 L) and plastic barrels (200 L). For the 7-day exposure, LC50 values were 0.047–0.049 ppm and LC99 were 0.144–0.151 ppm. For 14-day exposure, these values were 0.002–0.005 ppm and 0.006–0.01 ppm respectively. For both species, IE99 was 0.001 ppb under semi-field conditions, and was effective for nearly 2 months. Novaluron concentration 0.01 ppb was effective up to 3 months, with an IE of 89–95%. Authorities should critically review a reduction of the presently recommended field dosage of 200 ppm novaluron by × 100 or more. This would provide the same efficacy but mitigate environmental pollution, development of vector resistance, and financial losses.
A novel CT-based radiomics model for predicting response and prognosis of chemoradiotherapy in esophageal squamous cell carcinoma
Authors: Kasai Akinari , Miyoshi Jinsei , Sato Yasushi , Okamoto Koichi , Miyamoto Hiroshi , Kawanaka Takashi , Tonoiso Chisato , Harada Masafumi , Goto Masakazu , Yoshida Takahiro , Haga Akihiro , Takayama Tetsuji 
Published: January 2024

AbstractNo clinically relevant biomarker has been identified for predicting the response of esophageal squamous cell carcinoma (ESCC) to chemoradiotherapy (CRT). Herein, we established a CT-based radiomics model with artificial intelligence (AI) to predict the response and prognosis of CRT in ESCC. A total of 44 ESCC patients (stage I-IV) were enrolled in this study; training (n = 27) and validation (n = 17) cohorts. First, we extracted a total of 476 radiomics features from three-dimensional CT images of cancer lesions in training cohort, selected 110 features associated with the CRT response by ROC analysis (AUC ≥ 0.7) and identified 12 independent features, excluding correlated features by Pearson’s correlation analysis (r ≥ 0.7). Based on the 12 features, we constructed 5 prediction models of different machine learning algorithms (Random Forest (RF), Ridge Regression, Naive Bayes, Support Vector Machine, and Artificial Neural Network models). Among those, the RF model showed the highest AUC in the training cohort (0.99 [95%CI 0.86–1.00]) as well as in the validation cohort (0.92 [95%CI 0.71–0.99]) to predict the CRT response. Additionally, Kaplan-Meyer analysis of the validation cohort and all the patient data showed significantly longer progression-free and overall survival in the high-prediction score group compared with the low-prediction score group in the RF model. Univariate and multivariate analyses revealed that the radiomics prediction score and lymph node metastasis were independent prognostic biomarkers for CRT of ESCC. In conclusion, we have developed a CT-based radiomics model using AI, which may have the potential to predict the CRT response as well as the prognosis for ESCC patients with non-invasiveness and cost-effectiveness.
The physical properties and photocatalytic activities of green synthesized ZnO nanostructures using different ginger extract concentrations
Authors: Aliannezhadi Maryam , Mirsanaee Seyedeh Zahra , Jamali Mohaddeseh , Shariatmadar Tehrani Fatemeh 
Published: January 2024

AbstractThe green synthesis method which is aligned with the sustainable development goals (SDGs) theory, is proposed to synthesize ZnO nanoparticles using ginger extract to treat the acidic wastewater and acidic factory effluent as a current challenge and the effects of the concentration of extracts on the synthesized ZnO nanostructures are investigated. The results declare that the single-phase hexagonal ZnO is formed using ginger extract concentration of less than 25 mL and the crystallite size of green synthesized ZnO NPs increased with increasing the concentration of ginger extract. Also, the significant effects of ginger extract concentration on the morphology of nanoparticles (nanocone, nanoflakes, and flower-like) and the particle size are demonstrated. The low concentration of ginger extract leads to the formation of the ZnO nanoflakes, while the flower-like structure is gradually completed by increasing the concentration of the ginger extract. Furthermore, significant changes in the specific surface area (SSA) of the samples are observed (in the range of 6.1–27.7 m2/g) by the variation of ginger extract concentration and the best SSA is related to using 10 mL ginger extract. Also, the strong effect of using ginger extract on the reflectance spectra of the green synthesized ZnO NPs, especially in the UV region is proved. The indirect (direct) band gap energies of the ZnO samples are obtained in the range of 3.09–3.20 eV (3.32–3.38 eV). Furthermore, the photocatalytic activities of the samples for the degradation of methylene blue indicate the impressive effect of ginger extract concentration on the degradation efficiency of ZnO nanoparticles and it reaches up to 44% and 83% for ZnO NPs prepared using 5 mL ginger extract in a pH of 4.3 and 5.6, respectively. This study provided new insights into the fabrication and practical application of high-performance ZnO photocatalysts synthesized using ginger extract in degrading organic pollutants in an acidic solution.
Acute effects of high intensity interval training versus moderate intensity continuous training on haemostasis in patients with coronary artery disease
Authors: Košuta Daniel , Novaković Marko , Božič Mijovski Mojca , Jug Borut 
Published: January 2024

AbstractExercise training is associated with an acute net increase in coagulation, which may increase the risk of atherothrombosis in coronary artery disease (CAD) patients. We sought to compare the acute haemostatic effects of a bout of moderate-intensity continuous (MICT) and high-intensity interval training (HIIT) in patients with CAD. Patients after a recent myocardial infarction were randomized into a HIIT or MICT session of exercise training on a stationary bike. Blood was sampled at baseline, after the exercise bout and after a one-hour resting period. We measured overall haemostatic potential (OHP), overall coagulation potential (OCP), fibrinogen, D-dimer and von Willebrand factor (vWF) and calculated overall fibrinolytic potential (OFP). Linear mixed models for repeated measures were constructed to assess the treatment effect. A total of 117 patients were included. OCP, OHP, fibrinogen, D-dimer and vWF significantly increased after exercise and returned to baseline after a one-hour rest, OFP decreased after exercise and returned to baseline levels after a one-hour rest. Linear mixed models showed a significant difference between HIIT and MICT in fibrinogen (p 0.043) and D-dimer (p 0.042). Our study has shown that an exercise bout is associated with a transient procoagulant state in patients with CAD, with similar exercise-induced haemostatic changes for HIIT and MICT.
Past volcanic activity predisposes an endemic threatened seabird to negative anthropogenic impacts
Authors: Teixeira Helena , Le Corre Matthieu , Michon Laurent , Nicoll Malcolm A. C. , Jaeger Audrey , Nikolic Natacha , Pinet Patrick , Couzi François-Xavier , Humeau Laurence 
Published: January 2024

AbstractHumans are regularly cited as the main driver of current biodiversity extinction, but the impact of historic volcanic activity is often overlooked. Pre-human evidence of wildlife abundance and diversity are essential for disentangling anthropogenic impacts from natural events. Réunion Island, with its intense and well-documented volcanic activity, endemic biodiversity, long history of isolation and recent human colonization, provides an opportunity to disentangle these processes. We track past demographic changes of a critically endangered seabird, the Mascarene petrel Pseudobulweria aterrima, using genome-wide SNPs. Coalescent modeling suggested that a large ancestral population underwent a substantial population decline in two distinct phases, ca. 125,000 and 37,000 years ago, coinciding with periods of major eruptions of Piton des Neiges. Subsequently, the ancestral population was fragmented into the two known colonies, ca. 1500 years ago, following eruptions of Piton de la Fournaise. In the last century, both colonies declined significantly due to anthropogenic activities, and although the species was initially considered extinct, it was rediscovered in the 1970s. Our findings suggest that the current conservation status of wildlife on volcanic islands should be firstly assessed as a legacy of historic volcanic activity, and thereafter by the increasing anthropogenic impacts, which may ultimately drive species towards extinction.
ChemRAP uncovers specific mRNA translation regulation via RNA 5′ phospho-methylation
Authors: Ipas Hélène , Gouws Ellen B , Abell Nathan S , Chiou Po-Chin , Devanathan Sravan K , Hervé Solène , Lee Sidae , Mercado Marvin , Reinsborough Calder , Halabelian Levon , Arrowsmith Cheryl H , Xhemalçe Blerta 
Published: January 2024

Abstract5′-end modifications play key roles in determining RNA fates. Phospho-methylation is a noncanonical cap occurring on either 5′-PPP or 5′-P ends. We used ChemRAP, in which affinity purification of cellular proteins with chemically synthesized modified RNAs is coupled to quantitative proteomics, to identify 5′-Pme “readers”. We show that 5′-Pme is directly recognized by EPRS, the central subunit of the multisynthetase complex (MSC), through its linker domain, which has previously been involved in key noncanonical EPRS and MSC functions. We further determine that the 5′-Pme writer BCDIN3D regulates the binding of EPRS to specific mRNAs, either at coding regions rich in MSC codons, or around start codons. In the case of LRPPRC (leucine-rich pentatricopeptide repeat containing), a nuclear-encoded mitochondrial protein associated with the French Canadian Leigh syndrome, BCDIN3D deficiency abolishes binding of EPRS around its mRNA start codon, increases its translation but ultimately results in LRPPRC mislocalization. Overall, our results suggest that BCDIN3D may regulate the translation of specific mRNA via RNA-5′-Pme.
Interferon-epsilon is a novel regulator of NK cell responses in the uterus
Authors: Mayall Jemma R , Horvat Jay C , Mangan Niamh E , Chevalier Anne , McCarthy Huw , Hampsey Daniel , Donovan Chantal , Brown Alexandra C , Matthews Antony Y , de Weerd Nicole A , de Geus Eveline D , Starkey Malcolm R , Kim Richard Y , Daly Katie , Goggins Bridie J , Keely Simon , Maltby Steven , Baldwin Rennay , Foster Paul S , Boyle Michael J , Tanwar Pradeep S , Huntington Nicholas D , Hertzog Paul J , Hansbro Philip M 
Published: January 2024

AbstractThe uterus is a unique mucosal site where immune responses are balanced to be permissive of a fetus, yet protective against infections. Regulation of natural killer (NK) cell responses in the uterus during infection is critical, yet no studies have identified uterine-specific factors that control NK cell responses in this immune-privileged site. We show that the constitutive expression of IFNε in the uterus plays a crucial role in promoting the accumulation, activation, and IFNγ production of NK cells in uterine tissue during Chlamydia infection. Uterine epithelial IFNε primes NK cell responses indirectly by increasing IL-15 production by local immune cells and directly by promoting the accumulation of a pre-pro-like NK cell progenitor population and activation of NK cells in the uterus. These findings demonstrate the unique features of this uterine-specific type I IFN and the mechanisms that underpin its major role in orchestrating innate immune cell protection against uterine infection.
Adaptive multi‐level differential coupling control strategy for dual‐motor servo synchronous system based on global backstepping super‐twisting control
Authors: Wang Taoyu , Sun Shiyan 
Published: January 2024

AbstractA new dual‐motor servo steering system with improved reliability and safety is proposed. But, despite the advantages of the proposed servo system, it is strongly coupled, non‐linear and multivariable, where the biggest challenge lies in its tracking and synchronization control. To improve the tracking and synchronization control performance of the proposed servo system, a tracking and synchronization control strategy based on backstepping super‐twisting control and multi‐level differential coupling is presented. First, a parallel model of the dual‐motor is established. Then, backstepping and super‐twisting control algorithms are integrated while adaptively optimizing key parameters to ensure the robustness and tracking performance of each motor. After that, an angular synchronization controller with multi‐level differential coupling and backstepping super‐twisting algorithm is proposed to compensate for the synchronization error of the dual‐motor system caused by parameter uncertainty. Finally, the simulation is carried out using Simulink to verify the effectiveness of the proposed control strategy.
Comparing reference‐free WiFi radar sensing approaches for monitoring people and drones
Authors: Di Seglio Marco , Filippini Francesca , Bongioanni Carlo , Colone Fabiola 
Published: January 2024

AbstractThe use of WiFi signals for sensing purposes has attracted a lot of interest from both the radar and communications communities and several techniques have been explored. In the attempt of meeting the requirements for small sensor size, compactness, and easy deployment, the authors consider reference‐free approaches, namely approaches that do not require a good copy of the transmitted waveform to be available at the radar receiver. To this end, the authors first resort to a passive radar‐based processing scheme that only exploits the invariant a priori known initial portion of the physical layer protocol data unit, that is, the PHY Preamble, and its limitations in practical applications is investigated. Specifically, the authors show that, with this approach, an accurate time, phase, and frequency synchronization is essential and a possible strategy is investigated. As an alternative solution the authors consider a forward scatter radar‐based approach where only the amplitude modulation of the received signal is exploited to detect the presence of a moving target thus avoiding the need to know the transmitted signal. For the first time, the authors comparatively investigate advantages and drawbacks of the two reference‐free approaches and present practical strategies to handle the limitations observed. The results are reported for experimental tests with people and drones using WiFi transmissions in the 2.4 and 5 GHz band.
An Efficient Access to Heteroaryl/Aryl-Annulated Pyridine Derivatives and a Study of Their Mosquito Larvicidal Activity Against Dengue Vector
Authors: Pal Pamela , Show Sayanti , Roy Priya , Ghosh Tapas , Nandi Raj K. , Das Sukanya , Bhakta Sayantika , Mondal Swarupa 
Published: January 2024

AbstractWe report a convergent synthesis of heteroaryl/aryl-annulated pyridine and quinoline derivatives by a metal-free Povarov reaction. para-Toluene sulfonic acid was used as the catalyst in this reaction, which produced the products in good yields from the corresponding aromatic amines and ethyl vinyl ether. A pyridocoumarin and a pyridopyrimidine product were evaluated for their mosquito larvicidal activity against the third instar larvae of the dengue vector mosquito Aedes aegypti. Examination of morphological changes in the larvae showed damage to the target body part after treatment with both the pyridocoumarin and pyridopyrimidine products at the LC50 concentrations.
Efficacy and safety of a new low-volume PEG with citrate and simethicone bowel preparation for pediatric elective colonoscopy: Phase 3 RCT
Authors: Russo Giusy , Alvisi Patrizia , Romano Claudio , Angelino Giulia , Lemale Julie , Lachaux Alain , Lionetti Paolo , Veereman Genevieve , Ruggiero Cosimo , Padovani Michela , Tacchi Raffaella , Cenci Fabio , Cucchiara Salvatore , Oliva Salvatore 
Published: January 2024

Abstract
Background and study aims Currently available polyethylene glycol (PEG)-based preparations continue to represent a challenge in children. The aim of this study was to compare the efficacy and safety of a new low-volume PEG preparation with a conventional PEG-electrolyte solution (PEG-ES) in children and adolescents.
Patients and methods This was a multicenter, randomized, observer-blind, parallel-group, phase 3 clinical trial, where patients were randomized between PMF104 (Clensia) and a conventional PEG-ES (Klean-Prep), and stratified by age stratum (2 to <6; 6 to < 12;12 to <18 years). The primary endpoint was to test the non-inferiority of PMF104 versus PEG-ES, in terms of colon cleansing. Safety, tolerability, acceptability, palatability, and compliance were also assessed. Efficacy endpoints were analyzed in the per protocol set (PPS) and full analysis set (FAS) and safety and tolerability endpoints in the safety set (SAF).
Results Of the 356 patients enrolled, 258 were included in the PPS, 346 in the FAS, and 351 in the SAF. Non-inferiority of PMF104 was confirmed for children aged > 6 years and for all age groups in PPS and FAS, respectively. Optimal compliance was reported more frequently in the PMF104 than in the PEG-ES group, in both PPS (86.1% vs. 68.4%) and FAS (82.9% vs. 65.3%).Both preparations were equally safe and tolerable. Palatability and acceptability were considered better in the PMF104 group than in the PEG-ES group (27.1% vs. 15.3% and 15.3% vs. 3.5%, respectively).
Conclusions In children aged 6 to 17 years, the new low-volume product PMF104 is non-inferior to the reference PEG-ES in terms of bowel cleansing, safety, and tolerability, with slightly better results in compliance, palatability, and acceptability.
Advanced Technologies for Studying Microbiome–Female Reproductive Tract Interactions: Organoids, Organoids-on-a-Chip, and Beyond
Authors: Kaya Yosun A. , de Zoete Marcel R. , Steba Gaby S. 
Published: January 2024

AbstractThe female reproductive tract (FRT) is home to diverse microbial communities that play a pivotal role in reproductive health and disorders such as infertility, endometriosis, and cervical cancer. To understand the complex host–microbiota interactions within the FRT, models that authentically replicate the FRT's environment, including the interplay between the microbiota, mucus layer, immune system, and hormonal cycle, are key. Recent strides in organoid and microfluidic technologies are propelling research in this domain, offering insights into FRT–microbiota interactions and potential therapeutic avenues. This review delves into the current state of FRT organoid models and microbe integration techniques, evaluating their merits and challenges for specific research objectives. Emphasis is placed on innovative approaches and applications, including integrating organoids with microfluidics, and using patient-derived biobanks, as this offers potential for deeper mechanistic insights and personalized therapeutic strategies. Modeling various FRT properties in organoids is explored, from encompassing age-related epithelial features, oxygen levels, and hormonal effects to mucus layers, immune responses, and microbial interactions, highlighting their potential to transform reproductive health research and predict possible outcomes.