bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low expression of miR125a-5p predicts sensitivity to sorafenib by upregulating STAT3 and HTATIP2 in hepatocellular carcinoma
Authors: Zhou Hongyuan , Yang Lu , Wang Yu , Zhang Qin , Zhang Zewu , Wang Yingying , Pan Zhaolong , Cao Junbo , Li Dongyang , Liu Chen , Sun Tao , Zhang Wei 
Published: December 2023

Abstract
Background
Sorafenib is a standard of care for systemic therapy of hepatocellular carcinoma (HCC). Further personalized treatment with sorafenib is warranted. The current study aims to identify predictive biomarkers for the efficacy of sorafenib and investigate the underlying mechanism.
Methods
MiRNA array was performed in formalin-fixed paraffin-embedded tumour from patients who received adjuvant sorafenib therapy after liver resection, including patients who had tumor recurrence within one year and patients without tumor recurrence after liver resection. In situ hybridization (ISH) was performed to evaluate the expression of miR-125a-5p, and the association of miR125a-5p with overall survival (OS) and recurrence free survival (RFS) was evaluated. LM3 with miR125a-5p overexpression and SMMC7721 with miR125a-5p knockdown were constructed using lentiviruses respectively. The sensitivity to sorafenib treatment were studied by in vitro CCK8 assay and in vivo xenograft tumor model. RNA-seq, luciferase reporter assay, real-time PCR and Western blot assays were performed to elucidate the underlying mechanism.
Results
MiR125a-5p was among the most significantly changed microRNAs and low expression of miR125a-5p was associated with increased sensitivity to sorafenib. In situ hybridization and survival analysis found that higher miR125a-5p was associate with improved OS and RFS for HCC patients after liver resection. We overexpressed miR125a-5p in LM3 HCC cells and knockdown miR-125a-5p in SMMC7721, respectively, and confirmed that miR125a-5p overexpression caused sorafenib resistance both in vivo and in vitro, while miR125a-5p suppression led to sensitivity to sorafenib treatment. RNAseq and pathway analysis revealed that STAT3 pathway was downregulated by miR125a-5p. STAT3 was also confirmed as a target of miR125a-5p by TargetScan prediction, and both STAT3 and HTATIP2 were downregulated by miR125a-5p in in-vitro experiment.
Conclusion
Low expression of miR125a-5p predicted sorafenib sensitivity by upregulating STAT3 and HTATIP2.
Assessing the Effectiveness of a Virtual Reality-Based Simulation Program for Mental Health Nursing Practicum
Authors: Kim Geun Myun , Lim Ji Young , Kim Juhang 
Published: December 2023

Abstract

Introduction:
To develop a virtual mental nursing practice program and verify its effectiveness.
Methods
Participants were fifty-four fourth grade nursing students who completed more than one semester of theoretical courses in mental nursing with no psychiatric nursing clinical practice experience. The program comprised six themes; its effect was analyzed using repeated measures ANOVA.
Results
There were significant effects between groups and time points in mental disease-related knowledge, learning flow, and learning satisfaction.
Conclusions
The simulation education program was effective as an intermediate educational space connecting theoretical and field education. It can enhance students’ ability and confidence to interact with patients and perform therapeutic nursing.
Development of an anti-Pfs230 monoclonal antibody as a Plasmodium falciparum gametocyte blocker
Authors: Cuccurullo Emilia C. , Dong Yuemei , Simões Maria L. , Dimopoulos George , Bier Ethan 
Published: December 2023

Abstract
Vector control is a crucial strategy for malaria elimination by preventing infection and reducing disease transmission. Most gains have been achieved through insecticide-treated nets (ITNs) and indoor residual spraying (IRS), but the emergence of insecticide resistance among Anopheles mosquitoes calls for new tools to be applied. Here, we present the development of a highly effective murine monoclonal antibody, targeting the N-terminal region of the Plasmodium falciparum gametocyte antigen Pfs230, that can decrease the infection prevalence by > 50% when fed to Anopheles mosquitoes with gametocytes in an artificial membrane feeding system. We used a standard mouse immunization protocol followed by protein interaction and parasite-blocking validation at three distinct stages of the monoclonal antibody development pipeline: post-immunization, post-hybridoma generation, and final validation of the monoclonal antibody. We evaluated twenty antibodies identifying one (mAb 13G9) with high Pfs230-affinity and parasite-blocking activity. This 13G9 monoclonal antibody could potentially be developed into a transmission-blocking single-chain antibody for expression in transgenic mosquitoes.
Piperine improves the health span of old Drosophila melanogaster
Authors: Lee Hye-Yeon , Lee Ji-Hyeon , Baek Jisun , Cho Kyung A , Min Kyung-Jin 
Published: December 2023

Abstract
Piperine, a dietary phytochemical isolated from the Piper species, has been used as a natural medicine for pain, flu, and fever in ancient China and India. Although the health benefits of piperine have been widely studied, research on its effect on aging is limited. This study aimed to determine whether piperine has the potential to mitigate aging-related changes in the fruit fly (Drosophila melanogaster), which is an excellent model organism for studies on aging. The experiments were conducted using the newly eclosed or 30-day-old D. melanogaster wild-type strain Cantonized-white. Piperine was dissolved in 99% ethanol and added to the sucrose-yeast medium at a final concentration of 10, 35, 70, or 100 µM. The study examined the effects of piperine supplementation on the lifespan of D. melanogaster and other physiological functions, such as fecundity, feeding, lipid content, and resistance to environmental stress. Log-rank tests, Shapiro-wilk test, F-test, t-test, or Wilcoxon rank sum test were used to analyze the data. Piperine failed to change the lifespan and body weight, but increased the fecundity and decreased the feeding rate in one-week-old flies. However, when piperine was fed to 30-day-old flies, it increased the lifespan of male flies and the fecundity and feeding rate of female flies. These results indicate that piperine can improve the health of aged flies. The findings suggest that piperine has age-dependent and sex-specific anti-aging effects in fruit flies.
Feasibility of a novel eHealth intervention for Parkinson’s disease targeting motor-cognitive function in the home
Authors: Sedhed Jenny , Johansson Hanna , Andersson Nina , Åkesson Elisabet , Kalbe Elke , Franzén Erika , Leavy Breiffni 
Published: December 2023

Abstract

Background Parkinson’s disease (PD) drastically affects motor and cognitive function, but evidence shows that motor-cognitive training improves disease symptoms. Motor-cognitive training in the home is scarcely investigated and eHealth methods can provide continual support for PD self-management. Feasibility testing is however required. Objective to assess the feasibility (i) recruitment capability ii) acceptability and suitability iii) demand and safety) of a home-based motor-cognitive eHealth exercise intervention in PD. Methods The 10-week intervention was delivered using the ExorLive® application and exercises were individually adapted and systematically progressed and targeted functional strength, cardiovascular fitness, flexibility, and motor-cognitive function. People with mild-to moderate PD were assessed before and after the intervention regarding; gait performance in single and dual-task conditions; functional mobility; dual-task performance; balance performance; physical activity level; health related quality of life and perceived balance confidence and walking ability; global cognition and executive function. Feasibility outcomes were continuously measured using a home-exercise diary and contact with a physiotherapist. Changes from pre- and post-intervention are reported descriptively. Results Fifteen participants (mean age 68.5 years) commenced and 14 completed the 10-week intervention. In relation to Interventionacceptability, 64% of the motor sessions and 52% of motor-cognitive sessions were rated as "enjoyable". Concerning suitability, the average level of exertion (Borg RPE scale) was light (11–12). Adherence was high, with 86% of all (420) sessions reported as completed. No falls or adverse events occurred in conjunction with the intervention. Conclusions This motor-cognitive eHealth home exercise intervention for PD was safe and feasible in terms of Recruitment capability, Acceptability, Safety and Demand. The intensity of physical challenge needs to be increased before testing in an efficacy trial. Trial registration: This trial is registered at Clinicaltrials.gov with NCT05027620.
An 8T and 10T static random access memory with bit-serial operations for in-memory computing
Authors: Xu Weidong , Lou Mian , Li Li , Zhang Kai , Gong Longqing 
Published: December 2023

Abstract

To address the issue of memory-computer integration still requiring field-programmable gate array (FPGA) assistance for nonconvolutional computation in the acceleration of deep neural networks, this study proposes a general hybrid static random-access memory in-memory computing (IMC) that combines transposed 8T and 10T units with vector-based, bit-serial in-memory arithmetic to support integer/decimal and positive/negative multiply-accumulate operations with various bit widths. This provides the necessary flexibility and programmability for the development of various software algorithms ranging from neural networks to signal processing. Furthermore, it reduces the transfer of data between the IMC and FPGA. The proposed design achieves an energy efficiency of 21.39 TOPS/W at 1.2 V and 500 MHz. This study presents a novel IMC design that supports flexible bit-width operations, enhancing the versatility of deep learning applications. This advancement has potential implications for various applications, paving the way for more efficient computing systems.
Vanishing Refuges: Unraveling the Climate Change Impact on the Critically Endangered Black Softshell Turtle Nilssonia nigricans in Northeastern India
Authors: Vishnu C.S. , Das Priyanka , M.Rajkumar  , Gopi G.V. 
Published: December 2023

Abstract
Reptilian species can be regarded as a bioindicator of climate change. However, limited studies are available on the effect of climate change on the distribution of reptiles in India. Nilssonia nigricans is a Critically Endangered Soft-shell Turtle found in Northeastern India, facing severe threats due to the Anthropocene. Previously, this species was considered extinct in the wild; however, recently, some populations have been discovered in the Brahmaputra Valley. In this context, a study was initiated to understand the impact of climate change on the distribution of N. nigricans. Maximum Entropy (MaxEnt) is employed to predict the potential distribution range of this species for two time periods: the 2050s (2041–2060) and the 2070s (2061–2080) under both RCP 4.5 and RCP 8.5 scenarios. The study found that the Mean Temperature of the Wettest Quarter, Elevation and Precipitation Seasonality will be the major factors that determine the distribution of N. nigricans. The model indicated that under current conditions, 21.78% of the study area provides a suitable habitat for N. nigricans. Future predictions suggest a potential range contraction of 16.09% during the 2050s under RCP 4.5 and an increase of 25.83% in the 2050s under the RCP 8.5 scenario. However, in the 2070s, the habitat range of this species may decline to 10.77% under RCP 4.5 and 19.97% under RCP 8.5. The comparison of various RCP scenarios illustrated that the habitat range is shrinking under RCP 4.5 scenarios. Among the states, Assam is the only place that shows the highest potential for suitable sites, covering an area of 58,535 km2 (58.47%), while the remaining 41,581 km2 (41.53%) is deemed unsuitable for its current distribution. The study reveals that N. nigricans serves as a bioindicator of climate change in Northeast India, and the study's results will be helpful in creating conservation and management attention for the species.
A DICOM-based Standard for Quantitative Physical Rehabilitation
Authors: Matteo Alessandro Di , Lozzi Daniele , Mignosi Filippo , Polsinelli Matteo , Placidi Giuseppe 
Published: December 2023

Abstract
Physical rehabilitation (PR) is crucial in medicine that, yet to date, often relies on qualitative rather than objectively quantifiable data. With the recent scientific and technological advances, several instruments and methods have been proposed aimed at measuring and evaluating rehabilitation objectively, for quantitative PR (QPR), though without a standardized data format. The lack of a standard data format implies several issues, including the inability to compare datafrom different devices, create patient history, perform temporal evaluation or inter-patient comparisons, share data, create and use common evaluation scalesfor therapists, calculate statistics, etc. This lack is also an obstacle to the diffusion of artificial intelligence in rehabilitation, due to the data inhomogeneity,increasing uncertainty, and subjectivity. Digital Imaging and COmmunicationsin Medicine (DICOM) is an affirmed standard, born for medical imaging but recently extended to other fields of medicine, with the primary scope to share data from different devices, handle different associated processes, and assure interoperability among systems and specialists, by generating structured data and documents. In this article, a DICOM Information Object Definition (IOD) is proposed for QPR data. Furthermore, as an example, the proposed DICOM-like standard is applied for managing data from QPR of the hand, generated by the Virtual Glove (VG) device, a multi-source hand-tracking device, and results are discussed.
Structural and Molecular Dynamic Simulation Studies of CBL-Interacting Protein Kinase CIPK and its Complexes Related to Plant Salinity Stress
Authors: Das Prabir Kumar , Bhatnagar Tanya , Banik Sanhita , Majumdar Sambit , Dutta Debajyoti 
Published: December 2023

Abstract
Calcium-dependent signaling in plants is responsible for several major cellular events, including the activation of the salinity-responsive pathways. Calcium binds to calcineurin B-like protein (CBL), and the CBL-Ca2+ binds to CBL-interacting protein kinase (CIPK). The CBL-CIPK complex enhances the CIPK interaction with an upstream kinase. The upstream kinase phosphorylates CIPK that, in turn, phosphorylates membrane transporters. Targeted membrane transporter phosphorylation influences its activity and kick-starts many downstream functions, such as balancing the cytosolic Na+-to-K+ ratio. The CBL-CIPK interaction is pivotal for Ca2+-dependent salinity stress signaling. The plant contains multiple CBL and CIPK genes coded in their genomes. Hence, different yet specific combinations of CBL and CIPK are responsible for targeting particular ion transporters. Here, we present the computationally predicted structures of autoinhibited CIPK24 and CIPK24-CBL4 complex. The models are supported by the available structural and functional data. Models are energy-minimized and subjected to molecular dynamics (MD) simulations. MD simulations enabled us to predict the importance of conserved residues of the proteins. Finally, the work is extended to predict the CIPK24-CBL4 complex with the upstream kinase GIRK2. MD simulation on the ternary complex structure enabled us to identify the critical CIPK24-GIRK2 interactions. Together, these data could be used to engineer the CBL-CIPK interaction network for developing salt tolerance in crops.
Nexus between carbon emissions, renewable energy, technological innovation, and economic growth in the G7 economies: an econometric analysis
Authors: Liu Jianhua , Rasheed Mohsin 
Published: December 2023

Abstract
This research investigates the relationship among CO \(_2\) emissions, economic growth, technological innovation, renewable energy consumption, and the Environmental Kuznets Curve (EKC) in G7 countries from 1990 to 2022. The overarching objective is to uncover specific short-run and long-run associations between these variables, examining immediate impacts and long-term effects. With global concerns related to climate change and concerted international efforts to reduce CO$_2$, this study explores the critical dynamics between economic growth and environmental sustainability. The methods include panel unit root tests, cointegration analysis, causality tests, and AutoRegressive Distributed Lag (ARDL) models, chosen for their capacity to offer a thorough understanding of the relationships between the variables. The finding indicates a stable equilibrium between GDP and CO$_2$ emissions within G7 nations, signifying a crucial aspect of global climate change. Specifically, the results highlight that the relationship is statistically significant for the USA, Canada, Germany, and the UK while being deemed insignificant for Italy, France, and Japan. This study suggests a complex relationship that extends beyond short-run fluctuations. In the short term, an observed inverse correlation emphasizes the need for agile policy strategies to balance economic growth and environmental concerns. Promoting renewable energy sources and strategically increasing investment in research and development have policy implications that can help make economic growth and environmental concerns more sustainable and balanced.
The Effect of Fiber-Adhesive Composite on Bond Strength of Plastic Plates- Part IV: The Effect of Temperature on the Mechanical Behavior and Mechanical Properties
Authors: Noaman Abdelaziz 
Published: December 2023

Abstract
Composite truss core sandwich structures have the significant advantage in astronautic and aeronautic applications owing to their high specific stiffness and strength. In recent years, different composite sandwich panels have been designed, tested, and analyzed. The mechanical properties of composite materials may suffer when the material is exposed to high temperature, high humidity environments. Glass fiber reinforced plastics composite is extensively used as a structural material for pools, oil pipes and tanks because it has good corrosion resistance properties. In this work, the effect of temperature on PVC/PVC sandwich plates having 0.95 Wt.% glass fiber blended with different types of adhesives (epoxy, polyester, and polyvinyl chloride) is studied. Different temperatures (35 oC, 45 oC, 55 oC, 65 oC and 75 oC) as well as three times of exposure (2 hrs.,4 hrs. and 6 hrs.) is adopted. The mechanical properties such as tensile strength, ultimate strength, modulus of elasticity, energy to fracture, impact energy and adhesion shear at different temperatures are studied. The activation energy of PVC /PVC sandwich plates having glass fiber as reinforced material blended different adhesives was studied It has been found that the mechanical properties of PVC /PVC sandwich plates having 0.95 Wt.% glass fiber blended with different types of adhesives the mechanical properties are affected by temperature. In addition, it has been found that the activation energy of PVC /PVC sandwich plates having glass fiber as a reinforced material blended with polyvinyl acetate is the largest compared with epoxy and polyester.
The Combined Influence of Spin and Roughness Frequency on Sphere Aerodynamics
Authors: Elliott Jack , Smith Lloyd , Lyu Bin , Smith Barton 
Published: December 2023

Abstract
In this paper, the lift and drag of spinning spheres roughened with macro roughness elements are examined. The velocity field of these same spheres in flight is measured with Particle Image Velocimetry (PIV). Several spheres with varying roughness are examined at various spin rates and fixed Reynolds number. Unlike previous studies where the roughness height is varied, in the present work the number of roughness elements is varied. The PIV datasets are used to determine the boundary layer separation points for each case. Comparing the lift and drag to the separation points reveals that 1) the separation points become more asymmetric with spin (the Magnus effect), 2) The drag increases with the size of the wake and 3) the drag increases with the asymmetry of the separation points, meaning that lift on spheres correlates to increased drag. Scant evidence of this third effect has been reported previously. Additionally, it is shown that, counter to smooth spheres, the force transmitted to the surface through the roughness elements leads to significant drag. The drag is shown to increase with the number of roughness elements while the lift decreases. Results have implications for understanding aerodynamic forces on bluff bodies with roughness and passive control of aerodynamic forces through roughness element frequency rather than the traditional roughness height.
Phenological variations of anemophilous species in an Atlantic-influenced Mediterranean environment: a long term study
Authors: Montiel Nuria , Hidalgo Pablo J. , Adame José Antonio , González-Minero Francisco 
Published: December 2023

Abstract

Numerous studies have shown that climate change is having an impact on the pollen production and emission of various anemophilous plant species, both herbaceous and woody. This study attempts to elucidate whether this trend, highly marked in Europe, is also occurring with species in the surrounding of the city of Huelva (south-western Spain), an area with a milder climate than the Mediterranean littoral due to the influence of the Atlantic Ocean. For this purpose, the factors influencing the main pollen season were studied for all local taxa with an occurrence of more than 1 % of the total, over the period 1993-2022, using an airborne pollen trap to determine the pollen concentration. A trend analysis of meteorological variables potentially affected by climate change, such as temperature (maximum, minimum and average), precipitation and the number of hours of insolation per day was also carried out.
Our results indicate that there is significant variation between herbaceous and woody taxa. Woody taxa show a trend towards increased pollen production, as noted in other areas in Europe, associated to temperature in previous seasons. By contrast, herbaceous taxa, are more dependent on meteorological variables, showing an acceleration in the end date of the main pollen season correlated with temperature and insolation. In conclusion, herbaceous taxa are the more sensitive group to climate alterations, and may be an immediate indicator of the consequences of climate change on biodiversity in this representative area of the Atlantic-influenced Mediterranean region.Our results indicate that there is significant variation between herbaceous and woody taxa. Woody taxa show a trend towards increased pollen production, as noted in other areas in Europe, associated to temperature in previous seasons. By contrast, herbaceous taxa, are more dependent on meteorological variables, showing an acceleration in the end date of the main pollen season correlated with temperature and insolation. In conclusion, herbaceous taxa are the more sensitive group to climate alterations, and may be an immediate indicator of the consequences of climate change on biodiversity in this representative area of the Atlantic-influenced Mediterranean region.