bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of Polygala-Acorus in Treating Autism Spectrum Disorder Based on Network Pharmacology and Molecular Docking
Authors: Chen Haozhi , Zhou Changlin , Li Wen , Bian Yaoyao 
Published: December 2023

Background:
Recent epidemic survey data have revealed a globally increasing prevalence
of autism spectrum disorders (ASDs). Currently, while Western medicine mostly uses a
combination of comprehensive intervention and rehabilitative treatment, patient outcomes remain
unsatisfactory. Polygala-Acorus, used as a pair drug, positively affects the brain and kidneys,
and can improve intelligence, wisdom, and awareness; however, the underlying mechanism
of action is unclear.

Objective:
We performed network pharmacology analysis of the mechanism of Polygala–
Acorus in treating ASD and its potential therapeutic effects to provide a scientific basis for the
pharmaceutical’s clinical application.

Methods:
The chemical compositions and targets corresponding to Polygala–Acorus were obtained
using the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis
Platform, Chemical Source Website, and PharmMapper database. Disease targets in ASD were
screened using the DisGeNET, DrugBank, and GeneCards databases. Gene Ontology functional
analysis and metabolic pathway analysis (Kyoto Encyclopedia of Genes and Genomes) were
performed using the Metascape database and validated via molecular docking using AutoDock
Vina and PyMOL software.

Results:
Molecular docking analysis showed that the key active components of Polygala-
Acorus interacted with the following key targets: EGFR, SRC, MAPK1, and ALB. Thus, the
key active components of Polygala-Acorus (sibiricaxanthone A, sibiricaxanthone B tenuifolin,
polygalic acid, cycloartenol, and 8-isopentenyl-kaempferol) have been found to bind to EGFR,
SRC, MAPK1, and ALB.

Conclusion:
This study has preliminarily revealed the active ingredients and underlying mechanism
of Polygala-Acorus in the treatment of ASD, and our predictions need to be proven by
further experimentation.
Activation of the Complement Lectin Pathway by Iron Oxide Nanoparticles and Induction of Pro-inflammatory Immune Response by Macrophages
Authors: Khan Haseeb A. , Kishore Uday , Alrokayan Salman H. , Ibrahim Khalid E. 
Published: December 2023

Aims:
Nanoparticles are important agents for targeted drug delivery to tissues or organs,
or even solid tumour in certain instances. However, their surface charge distribution makes
them amenable to recognition by the host immune mechanisms, especially the innate immune
system, which interferes with their intended targeting, circulation life, and eventual fate in the
body. We aimed to study the immunological response of iron oxide nanoparticles (Fe-NPs) and
the role of the complement system in inducing an inflammatory cascade.

Background:
The complement system is an important component of the innate immune system
that can recognise molecular patterns on the pathogens (non-self), altered self (apoptotic and
necrotic cells, and aggregated proteins such as beta-amyloid peptides), and cancer cells. It is no
surprise that clusters of charge on nanoparticles are recognised by complement subcomponents,
thus activating the three complement pathways: classical, alternative, and lectin.

Objective:
This study aimed to examine the ability of Fe-NPs to activate the complement system
and interact with macrophages in vitro.

Methods:
Complement activation following exposure of macrophage-like cell line (THP-1) to
Fe-NPs or positive control was analysed by standard protocol. Real-time PCR was used for
mRNA-level gene expression analysis, whereas multiplex cytokine array was used for proteinlevel
expression analysis of cytokines and chemokines.

Results:
Fe-NPs activated all three pathways to a certain extent; however, the activation of the
lectin pathway was the most pronounced, suggesting that Fe-NPs bind mannan-binding lectin
(MBL), a pattern recognition soluble receptor (humoral factor). MBL-mediated complement
activation on the surface of Fe-NPs enhanced their uptake by THP-1 cells, in addition to dampening
inflammatory cytokines, chemokines, growth factors, and soluble immune ligands.

Conclusion:
Selective complement deposition (via the lectin pathway in this study) can make
pro-inflammatory nanoparticles biocompatible and render them anti-inflammatory properties.
Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols:
A Guideline for Regenerative Medicine
Authors: Elias Ferreira Stricker Priscila , de Oliveira Nathalia Barth , Felipe Mogharbel Bassam , Lührs Larissa , Irioda Ana Carolina , Abdelwahid Eltyeb , Regina Cavalli Luciane , Zotarelli-Filho Idiberto José , de Carvalho Katherine Athayde Teixeira 
Published: December 2023

Background::
This systematic review describes the most common methodologies for immortalizing
human and animal mesenchymal stem cells (MSCs). This study follows the rules of
PRISMA and is registered in the Institutional Review Board of PROSPERO International of systematic
reviews, numbered protocol code: CRD42020202465.

Method::
The data search systematization was based on the words “mesenchymal stem cell” AND
“immortalization.” The search period for publications was between 2000 and 2022, and the databases
used were SCOPUS, PUBMED, and SCIENCE DIRECT. The search strategies identified 384 articles:
229 in the SCOPUS database, 84 in PUBMED, and 71 in SCIENCE DIRECT. After screening
by titles and abstracts, 285 articles remained. This review included thirty-nine articles according to
the inclusion and exclusion criteria.

Result::
In 28 articles, MSCs were immortalized from humans and 11 animals. The most used immortalization
methodology was viral transfection. The most common immortalized cell type was the
MSC from bone marrow, and the most used gene for immortalizing human and animal MSCs was
hTERT (39.3%) and SV40T (54.5%), respectively.

Conclusion::
Also, it was observed that although less than half of the studies performed tumorigenicity
assays to validate the immortalized MSCs, other assays, such as qRT-PCR, colony formation in
soft agar, karyotype, FISH, and cell proliferation, were performed in most studies on distinct MSC
cell passages.
Advancements in Nanotechnology for Enhanced Antifungal Drug Delivery: A Comprehensive Review
Authors: Srivastava Rajat , Rawat Ajay Kumar Singh , Mishra Manoj Kumar , Patel Amit Kumar 
Published: December 2023

Abstract::
Infections caused by fungi can be mildly bothersome or fatal, causing life-threatening conditions
or even death. Antifungal drugs have used synthetic chemicals, organic compounds, and phytoconstituents
in their formulations to treat fungal infections. Research into novel antifungal drugs
has progressed more rapidly than into antibacterial treatments. This can be attributed to the low resistance
of fungal infections to antifungal bioactivities and the relatively low incidence of these diseases.
Carrier systems based on nanotechnology have generated much interest recently because of the
incredible potential of these systems. By using nanoarchitecture as a better carrier and drug delivery
system (DDS), we can have greater antifungal effectiveness, bioavailability, targeted action, and less
cytotoxicity, a development made possible using nanotechnology. This review discusses various
nanocarrier-based technologies in addition to other nanotechnological methods. These include liposomes,
transfersomes, ethosomes, niosomes, dendrimers, polymeric nanoparticles, polymer nanocomposites,
metallic nanoparticles, carbon nanomaterials, etc.

:
This review focused on general information regarding fungi infections, different antifungal agent
types and mechanisms of action, and an overview of formulation strategies such as nanotechnology
systems, which are frequently researched for antifungal therapies.

:
We concluded that new drug delivery systems are crucial to delivering antifungal medicines to their
target site with the optimum concentration. The researchers also concentrated on these innovative drug
delivery systems, which primarily focus on regulating and maintaining the release of antifungal drugs.
Recent Innovations and Patents of Lithium Extraction Techniques
from Various Lithium Bearing Solutions
Authors: Aljarrah Sewar , Al-Rawajfeh Aiman E. , Shahid Muhammad Kashif , Qamar Ul Islam  
Published: December 2023

Abstract:
Lithium, with its exceptional properties, such as remarkable redox activity and high
specific heat capacity, finds widespread applications in thermonuclear fusion reactors, ceramics,
batteries, aerospace, glass, metal additives, and photo-electricity. The growing demand for clean
technology, especially electric cars and energy storage, has led to a significant rise in global
lithium production. Brines now constitute a major portion of the world's lithium output, driving
research in lithium extraction and purification methods. This study examines recent innovative
research and patents, including various extraction techniques, sorbents, electrolysis-based approaches, and cost-effective methods. The study identifies gaps and limitations in existing lithium extraction technology and proposes future research areas to advance the field. The bibliographic analysis underscores the importance of further investigation to overcome current obstacles and drive progress in lithium extraction.
Assessment of serum calcium, magnesium and zinc levels in patients with type 2 diabetes mellitus in the Ukrainian population
Authors: Kravchenko V.І. , Ivaskiva K.Yu. , Andrusyshyna I.M. , Pankiv V.I. , Khalangot M.D. , Orlenko V.L. , Vasiuk V.L. 
Published: December 2023

Background. The research deals with the provision of calcium, magnesium and zinc in patients with type 2 diabetes mellitus (T2DM). The purpose was to investigate the serum content of calcium, magnesium and zinc in patients with type 2 DM. Materials and methods. The open-label controlled study included 27 patients with T2DM. The control group consisted of 12 people without endocrine and somatic diseases. 70 % of patients had newly diagnosed DM; in others, the disease duration was up to 5 years. Most of the examined were of mature age, on average 61.83 ± 5.52 years for patients and 55.25 ± 5.52 years for controls. In the group with T2DM, women accounted for 62.96 %, among controls — 63.64 %. Anthropometric measurements and glucose indicators were determined according to a standard procedure. Concentrations of calcium, magnesium, zinc in serum were evaluated by atomic optical emission spectrometry with inductively coupled plasma (PerkinElmer Optima 2100 DV ICP-OES, USA) according to the original procedure approved by the Kundiiev Institute of Occupational Health of the NAMS of Ukraine. Results. The level of fasting blood sugar ranged from 7.1 to 17.2 mmol/l and indicated the presence of T2DM, glycated hemoglobin was from 7.1 to 11.2 %. According to anthropometric data, the group of patients almost completely corresponded to the control group. Although a slight increase in body weight and waist circumference was registered already at the stage of initial diabetes, the level of triglycerides increased significantly, and the level of high-density lipoprotein decreased. A significant reduction in the levels of calcium and magnesium in the blood serum of patients with T2DM was found. The zinc content was at the level of the lower edge of the reference value in 44 % of patients, in the control group — only in 16.6 % of cases. There were no significant differences in this indicator in the observation groups. A high correlation was found between the studied parameters in the blood, as well as an inverse correlation between the fasting glucose level and the content of calcium and magnesium. Conclusions. A significant decrease in the level of calcium and magnesium in the blood of Ukrainian patients with type 2 diabetes mellitus was found. There is a high correlation between serum calcium, magnesium and zinc levels in patients with T2DM. Significant risks of diabetes were revealed when the serum concentration of calcium and magnesium decreased.
Analysis of the selenoprotein P (rs7579) gene polymorphism and expression in patients with chronic pancreatitis combined with hypothyroidism
Authors: Ratsa V.V. , Fediv O.I. , Sydorchuk L.P. , Rossokha Z.I. , Sydorchuk O.I. , Stepan V.T. , Buzdugan I.O. 
Published: December 2023

Background. The specific role and place of genetic factors in the development of chronic pancreatitis and hypothyroidism, which determine the activity of glutathione antioxidant protection, have not been clarified. They are interconnected with changes in the fat and carbohydrate metabolism, and also affect the transport and signaling pathways of key nutrients for the work of the immune, endocrine and nervous systems. The purpose of the study is to investigate the selenoprotein P (SEPP1) (rs7579) gene polymorphism and expression in patients with chronic pancreatitis combined with hypothyroidism. Materials and methods. Forty-nine patients with chronic pancreatitis and hypothyroidism and 30 practically healthy individuals passed the screening stage. The SEPP1 (rs7579) gene polymorphism was determined by the polymerase chain reaction. Results. The study showed that out of 98 isolated alleles in patients with chronic pancreatitis and hypothyroidism and 60 alleles of the control group, the G allele of the SEPP1 gene (rs7579, 25191G/A) dominated over the A allele: in the examined patients — by 34.7 % (χ2 = 23.59; p < 0.001), in the practically healthy group — by 53.34 % (χ2 = 34.13; p < 0.001). At the same time, the relative frequency of individual genotypes, as well as wild-type and mutant alleles, probably did not differ between the experimental and control groups. The A allele of the SEPP1 gene (rs7579) slightly increases the risk of chronic pancrea­titis in the studied population, but non-significantly [risk ratio (RR) = 1.43; RR 95% CI (confidence interval): 0.91–2.26; odds ratio (OR) = 1.65; OR 95% CI: 0.88–3.08; p = 0.115]. Conclusions. In patients with chronic pancreatitis who are residents of Northern Bukovyna, mutation of the SEPP1 gene (rs7579, 25191G/A) in the homozygous state occurs with a frequency of 10.2 %, while it is absent in practically healthy people. In both groups, the G allele dominates over the A allele: in the examined patients — by 34.7 % (χ2 = 23.59; p < 0.001), in controls — by 53.34 % (χ2 = 34.13; p < 0.001). SEPP1 gene polymorphism (rs7579, 25191G/A) does not determine the risk of chronic pancreatitis in the population. However, the A allele increases the risk of hypothyroidism in chro­nic pancreatitis patients twice [OR = 2.0; OR 95% CI: 1.09–3.66; p = 0.023], with the lowest chances of its appearance in carriers of the G allele [OR = 0.50; OR 95% CI: 0.27–0.91; p = 0.023]. Mapping of the expression quantitative trait loci on both sides of the SEPP1 gene (rs7579) transcription start site evidenced 152 statistically significant cis-variants of rs7579 of the SEPP1 gene (SELENOP) associations with the expression of 20 genes in 35 different organs and tissues and 22 phenotypic traits.
Transformasi Makna Kata Serapan Bahasa Arab Pada Bahasa Indonesia Dalam Istilah Agama Islam dari Aspek Bahasa dan Budayanya
Authors: Hakami Fauzan , Kalimatun Nabil Muaz , Ramadhany Maghrevian , Zikra Laka , Khoiri Rahman Subhan 
Published: December 2023

This research aims to describe Arabic loan words in Indonesian which focuses on terms contained in the Islamic religion and analyze changes in meaning based on linguistic and cultural factors of the source language. This research uses a qualitative descriptive research approach, namely the Systematic Literature Review method with a number of research objects. The data sources used include previous research articles and journals that examine the transformation of language meaning.
The research results identified three types of meaning changes in Arabic vocabulary that were adopted into Indonesian. The three types of meaning changes include broad meaning changes, narrowed meaning changes, and total meaning changes. This research provides in-depth insight into how words in Arabic interact with the Islamic religion and are then absorbed into Indonesian, and how these meanings change over time and context in aspects of language and culture.
Implementasi Quantum Teaching Sebagai Upaya Meningkatkan Kemampuan Menulis Karya Ilmiah
Authors: Apriyadi Hendra 
Published: December 2023

Kemampuan menulis karya ilmiah memiliki peran krusial dalam pengembangan ilmu pengetahuan dan pendidikan. Oleh karena itu, penelitian ini bertujuan untuk menginvestigasi upaya meningkatkan kemampuan menulis karya ilmiah menggunakan Quantum Teaching sebagai pendekatan inovatif dalam pembelajaran mata kuliah bahasa Indonesia di program studi S1 Farmasi STIKes Muhammadiyah Tegal. Penelitian yang fokus pada Quantum Teaching. Quantum Teaching merupakan pendekatan pembelajaran yang mendorong pengembangan potensi individu, integrasi teknologi, serta pemahaman mendalam tentang materi pembelajaran. Instrumen yang digunakan untuk pengumpulan data yaitu berupa (pre-tes dan pos-tes) dan observasi dan wawancara. Data-data tersebut dianalisis secara kualitatif. Hasil dari penelitian tersebut menunjukkan bahwa terdapat efek positif dan implementasi metode quantum taching dalam kemampuan menulis karya ilmiah mahasiswa.
Vivências de professores/as de línguas responsivos/as e responsáveis
Authors: Santos Andre Cordeiro dos , Zozzoli Rita Maria Diniz , Silva Júnior Sílvio Nunes da 
Published: December 2023

Propomos, com este estudo, uma discussão sobre a constituição do/a professor/a de línguas responsivo/a e responsável, partindo das vivências desses sujeitos no cronotopo da pandemia, como parte de um projeto de pesquisa maior, sobre as vivências desses/dessas professores/as. Tendo como pressupostos teóricos escritos do chamado Círculo de Bakhtin e estudos sobre formação e vivências de professores/as, procuramos discutir aspectos da concepção de professor/a responsivo/a ativo/a e responsável, articulando os apontamentos teóricos e epistemológicos com alguns discursos de professores/as que vivenciaram a realidade pandêmica de ensino. Em linhas gerais, a investigação deixou perceptível que os desafios lançados à profissão docente, reforçados pelo cronotopo da pandemia, vêm evidenciando o caráter múltiplo das vivências do/a professor/a. Assim, aspectos oriundos de suas vivências pessoais e profissionais, que englobam discursivamente particularidades individuais e elementos que emergem do compartilhamento constante de dificuldades e avanços entre professores/as em espaços distintos, ora se mostram por meio do vivenciamento do nós do tipo coletivo, ora por meio do vivenciamento do nós do tipo individualista.
Taxation and Forensic Accounting: Informing Research and Practice
Authors: DiGabriele James A. , Antinozzi Hannah Smith , Russo Charles , Kunz Philip W. 
Published: December 2023

ABSTRACT
We synthesize academic research, court cases, and Internal Revenue Service directives related to taxation and forensic accounting to improve the understanding of the extant literature on central issues. We emphasize areas where there is a need for future research opportunities that will contribute to the literature in taxation and forensic accounting. We identify research opportunities in taxation and forensic accounting in critical areas, such as (1) tax issues in business valuation; (2) indirect methods of reconstructing taxable income, including tax fraud and evasion; (3) corporate culture and ethical issues; (4) fraud in tax factors of performance, risk, and disclosure; and (5) internal control and tax assessment. Generally, we find research on taxation and forensic accounting to be scant. This space creates an emerging venue for future research.
Produkcyjność soi uprawianej metodą proekologiczną i konwencjonalną w zależności od rozstawy rzędów
Authors: Gawęda Dr hab. prof. uczelni Dorota , Buczek Jan 
Published: December 2023

Soja (Glycine max (L.) Merr.) jest rośliną bobowatą cenioną m.in. za dużą wartość odżywczą nasion. Odgrywa ważną rolę w płodozmianie, poprawiając żyzność i strukturę gleby. Dlatego kluczowe znaczenie mają badania ukierunkowane na wyznaczenie optymalnych warunków agrotechnicznych dla uzyskania wysokich plonów tej rośliny. Celem przeprowadzonych badań była ocena plonowania dwóch odmian soi wysiewanych w zróżnicowanej rozstawie rzędów w warunkach uprawy metodą proekologiczną i konwencjonalną. Czteroletnie badania polowe udowodniły, że uprawa metodą konwencjonalną oddziaływała korzystniej niż proekologiczną na wielkość plonu nasion, obsadę roślin po wschodach, liczbę strąków z rośliny oraz liczbę i masę nasion z rośliny. W uprawie proekologicznej soja znacznie wyżej zawiązywała strąki. Lepiej plonującą okazała się odmiana Merlin niż Aldana (o 43,4%), u której korzystniej kształtowały się również elementy struktury plonu i łanu, z wyjątkiem masy 1000 nasion. Istotnie większy plon nasion soi uzyskano w rozstawie rzędów 22,5 cm niż 35 cm.
Prognostic value of C-reactive protein in dogs with elevated serum pancreatic lipase immunoreactivity concentrations
Authors: Oberholtzer Sydney M. , Cook Audrey K. , Gomez Robynne , Steiner Jörg M. 
Published: December 2023

Abstract

OBJECTIVE
To determine the prognostic value of serum C-reactive protein (CRP) in dogs with pancreatitis.

ANIMALS
503 client-owned animals with pancreatic lipase immunoreactivity (PLI) > 600 µg/L.

METHODS
Routine submissions to the Texas A&M Gastrointestinal Laboratory were monitored for canine samples with PLI > 600 µg/L. Clinics were emailed 2 weeks after PLI measurement and asked the following questions: (1) was the dog hospitalized, and (2) is the patient alive? If a response was received, serum CRP concentration was measured using leftover serum.

RESULTS
Paired PLI and CRP results were available for 503 dogs. Median PLI was 984 µg/L (range, 603 to 2,001 µg/L); median CRP was 9.9 mg/L (range, 9.9 to 395.3 mg/L; ref: < 10 mg/L). Inpatient care was provided to 136 dogs (27.0%); 49 dogs (9.7%) died or were euthanized. Median PLI values for dogs that died versus survived were similar. Median CRP was higher in hospitalized dogs (36.1 vs 9.9 mg/L; P < .0001) and those that died (37.2 vs 9.9 mg/L; P < .0001). Compared to dogs with CRP < 10 mg/L, those with CRP > 10 mg/L were 5.3 times more likely to die (CI, 2.7 to 10.2) and 5.7 times (CI, 3.7 to 8.7) more likely to be hospitalized.

CLINICAL RELEVANCE
In dogs with PLI > 600 µg/L, CRP > 10 mg/L was associated with increased risk of hospitalization or death. This biomarker may provide prognostic information in dogs with evidence of pancreatitis and guide decisions regarding hospitalization or referral.