bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychometric network analysis of the Intuitive Eating Scale‐2 in Chinese general adults
Authors: Ji Feng , Sun Hongyi , Barnhart Wesley R. , Cui Tianxiang , Cui Shuqi , Zhang Jihong , He Jinbo 
Published: February 2024

AbstractThe Intuitive Eating Scale‐2 (IES‐2) is a measure of intuitive eating behaviors that has been extensively validated, with traditional latent variable modeling approaches, in youth and adults from many different populations, including college students in China. However, there is still a lack of research on the psychometric properties of the IES‐2 in adults from the Chinese general population. Moreover, psychometric network analysis, as a complement to traditional latent variable modeling approaches, has not been used for examining the psychometric properties of the IES‐2. Thus, the present study used a psychometric network approach to evaluate the psychometric properties of the IES‐2 in Chinese adults from the general population. A sample of 700 Chinese general adults (50% women; Mage = 31.13 years, SD = 9.19) recruited online were included in the present study. Psychometric network analysis was performed. Exploratory graph analysis (EGA) identified four dimensions, which were well separated in the estimated network. The network structure showed excellent stability and metric measurement invariance (i.e., network loadings) across men and women. Furthermore, several items on the IES‐2 were identified as key nodes in the network of the IES‐2 that may be important for the development and maintenance of intuitive eating. For example, two items (i.e., “I trust my body to tell me when to eat,” and “I trust my body to tell me when to stop eating”) related to reliance on body cues were the most impactful nodes in the complete network. The findings of our study provide a greater understanding of the IES‐2 from the perspective of network analysis and have implications for applications of intuitive eating interventions for general populations.
Effect of iron powder on hydrogen sulfide reduction and phosphate removal in semi‐continuous anaerobic digestion treating primary sludge
Authors: Oh Seung‐Jun , Ha Seung‐Han , Noh Eui Jeong , Shin Seung Gu , Ahn Johng‐Hwa 
Published: February 2024

AbstractBACKGROUNDTo our best knowledge, no experimental study has previously investigated the simultaneous effects of adding Fe powder for the decrease of hydrogen sulfide (H2S) in biogas and the removal of phosphate (PO4‐P) in continuous anaerobic digestion (AD). This study investigated the effects of iron (Fe) powder (5 g L−1) in AD to remove H2S and PO4‐P. A comparative experiment was conducted using two semi‐continuous‐flow anaerobic digesters treating primary sludge at decreasing hydraulic retention times (HRTs) of 20 to 10 days.RESULTSThe addition of Fe powder to AD removed > 98% of H2S at all HRTs, and removed 64–72% of PO4‐P, with the highest removal efficiency at HRT = 10 d. The addition of Fe powder to AD had no noticeable effect on biogas production or pH, but it increased the CH4 content (62.7–66.0% in Fe‐treated vs 58.2–60.4% in the Control) partly due to the greater presence of Methanoregulaceae. The relative abundance of the microbial groups was more affected by HRT than by the addition of Fe powder. The presence of Fe powder notably increased the abundance of Spirochaetaceae and Syntrophaceae.CONCLUSIONAs shown, the addition of Fe powder can improve the management of both H2S in the biogas and PO4‐P in the digested sludge in the AD system. © 2024 Society of Chemical Industry (SCI).
ZnO nanomaterials with enhanced antimicrobial activity obtained by Eichhornia crassipes aqueous extract‐mediated synthesis
Authors: López González Rosendo , Gutiérrez Mirian Hernández , García Ruth Lezama , Gómez Cornelio Sergio Alberto , Lobato Carlos , Gómez‐Rivera Abraham , Quintana Owen Patricia , Alvarez Lemus Mayra Angélica 
Published: February 2024

AbstractBACKGROUNDZinc oxide nanoparticles (ZnO NPs) were obtained through precipitation synthesis, using aqueous water hyacinth leaf extract (Eichhornia crassipes) under alkaline conditions. The effect of the extract on the physicochemical and antimicrobial properties of the ZnO was evaluated starting from concentrated extract (labeled as E100) and performing two dilutions of this concentrated extract at 50% and 25% in water (E50, and E25).RESULTSThe FTIR spectra revealed strong peaks at ≈3440 cm−1, corresponding to vibrations from OH stretching, and medium‐intense peaks at 1384 and 1327 cm−1, assigned to CC, COOH and COC vibrations, which are related to the presence of the extract; a weak band at 884 cm−1 and a broad band in the region below 600 cm−1 indicate the presence of ZnO. The hydrodynamic size of the ZnOE100, ZnOE50 and ZnOE25 samples were 202, 244 and 313 nm, respectively. No significant variations on the Eg value were observed. Field emission scanning electron microscopy images showed that the ZnO NPs have triangular‐like shapes and agglomeration of the NPs synthesized for the ZnOE25 sample. The minimum inhibitory concentration (MIC) of the ZnO NPs showed no significant effects related to the concentration the plant extract used.CONCLUSIONSVariations in particle size and zeta potential can be introduced in ZnO by varying the content of the extract; the presence of organic groups from the extract, along with a positive zeta potential value, can promote the bactericide effect of ZnO nanomaterials prepared by bio‐assisted synthesis. © 2024 Society of Chemical Industry (SCI).
Enzymatic Knoevenagel condensation in dual‐functionalized water‐mimicking ionic liquids and tertiary amide solvents
Authors: Zhao Hua , Campbell Chante' D 
Published: February 2024

AbstractBACKGROUNDKnoevenagel condensation is an important tool for building carbon–carbon (CC) bonds, especially when catalyzed by enzymes to enable a potentially high chemo‐, regio‐ and/or stereoselectivity. Although many Knoevenagel condensation reactions are carried out in aqueous solutions, insoluble hydrophobic substrates often lead to poor catalytic efficiencies. The use of water‐miscible organic solvents improves the substrate solubilization, but usually induces activity suppression or inactivation of enzymes. There is a great need to develop alternative solvents for both substrate dissolution and enzyme compatibility in CC bond formation reactions.RESULTSOur group previously developed dual‐functionalized water‐mimicking ionic liquids (ILs) for the activation and stabilization of hydrolases (e.g. lipase and protease). In the present study, we evaluated the Knoevenagel condensation of 4‐chlorobenzaldehyde with acetylacetone, and found that porcine pancreas lipase in water‐mimicking ILs carrying ammonium, imidazolium and benzimidazolium cations enabled higher reaction rates (up to 3.22 μmol min−1 g−1 lipase) and better yields than tert‐butanol, glymes and [BMIM][Tf2N]. Interestingly, tertiary amide solvents such as N‐methyl‐2‐pyrrolidone (NMP), N,N‐dimethylformamide (DMF) and N,N‐dimethylacetamide (DMAc) led to 8.2‐ to 11.1‐fold increases in the initial rate (up to 35.66 μmol min−1 g−1 lipase) when compared with dual‐functionalized ILs, which is likely due to some synergistic effect of these tertiary amides with the lipase.CONCLUSIONDual‐functionalized ILs based on ammonium, imidazolium and benzimidazolium cations improved Knoevenagel condensation reaction rates and yields when compared with tert‐butanol and glymes. Tertiary amides (NMP, DMF and DMAc) significantly increased the reaction rate. © 2024 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Comparison and discussion of behavior and pathology of four kinds of cerebral palsy disease models
Authors: Xu Jinyan , Yan Siyang , Xia Chen , Xue Jianyi , Yu Wentao , Yan Yuanjie , Yin Zhenjin 
Published: February 2024

AbstractExplore the differences in behavioral and pathological manifestations of rat models of cerebral palsy made by different methods and discuss what types of studies these models are suitable for. Behavioral evaluation and pathological section observation were used to observe and evaluate the model. Conclusion: except for the absence of data of bilateral common carotid artery ligation rats, the other three methods could all achieve a successful cerebral palsy disease model for both behavioral and pathological. For researchers, the selection of intraperitoneal infection model in pregnant rats or unilateral ischemia and hypoxia model in infant rats is sufficient to meet the experimental needs, whereas the selection of the combined method for modeling does not show enough advantages, which not only causes the waste of financial and human resources but also increases the possibility of experimental error made by intervention factors.
Substantial gene expression shifts during larval transitions in the pearl oyster Pinctada margaritifera
Authors: Destanque T. , Le Luyer J. , Quillien V. , Sham Koua M. , Auffrey P. , Ky C.‐L. 
Published: February 2024

AbstractEarly development stages in marine bivalve are critical periods where larvae transition from pelagic free‐life to sessile mature individuals. The successive metamorphosis requires the expression of key genes, the functions of which might be under high selective pressure, hence understanding larval development represents key knowledge for both fundamental and applied research. Phenotypic larvae development is well known, but the underlying molecular mechanisms such as associated gene expression dynamic and molecular cross‐talks remains poorly described for several nonmodel species, such as P. margaritifera. We designed a whole transcriptome RNA‐sequencing analysis to describe such gene expression dynamics following four larval developmental stages:
d‐shape, Veliger, Umbo and Eye‐spot. Larval gene expression and annotated functions drastically diverge. Metabolic function (gene expression related to lipid, amino acid and carbohydrate use) is highly upregulated in the first development stages, with increasing demand from
d‐shape to umbo. Morphogenesis and larval transition are partly ordered by Thyroid hormones and Wnt signaling. While larvae shells show some similar characteristic to adult shells, the cause of initialization of biomineralization differ from the one found in adults. The present study provides a global overview of Pinctada margaritifera larval stages transitioning through gene expression dynamics, molecular mechanisms and ontogeny of biomineralization, immune system, and sensory perception processes.
Exploring genetic counselors' experiences with non‐paternity in clinical settings
Authors: Cunningham Emma , Hays Stephen , Wainstein Tasha , Zierhut Heather , Virani Alice , Tryon Rebecca 
Published: February 2024

AbstractNon‐paternity (NP) is a challenging dilemma faced by genetics providers and there is little consensus on whether this finding should be disclosed. Discussions in the literature are highly theoretical, with limited research regarding how disclosure decisions are enacted in practice. We explored genetic counselors' (GCs) clinical experiences with NP to understand if, how, and why this finding is communicated. Our semi‐structured interviews with genetic counselors in the United States and Canada were analyzed using reflexive thematic analysis to analyze data inductively, describe themes, and present a meaningful interpretation of the data. Eighteen participants who responded to list‐serv messages were interviewed. Our framework describes five salient themes: (1) GC‐lab relationship: the GCs awareness of laboratory processes such as quality control metrics that can uncover NP findings and the way in which a finding of NP was disclosed by the laboratory had an impact on disclosure decisions. This triggered a decision‐making trajectory that involved (2) consultation, (3) ethical reasoning, and (4) practical constraints. GCs frequently consulted other professionals during decision‐making. These conversations impacted disclosure decisions with some consultations carrying greater weight than others. GCs weighed moral concepts of patient autonomy, medical relevance, and preventing harm to rationalize decisions. Access to patients and documentation requirements often dictated how disclosure occurred. Finally, once a decision had been made and enacted, GCs used the experience to reconsider their approach to (5) consenting in future cases, with some GCs altering their pre‐test counseling to always include a discussion of NP. Although NP scenarios are frequently unique in context, our findings demonstrate several common decision‐making factors GCs harness to navigate the identification of NP through clinical genetic testing.
Characterization of the m6A/m1A/m5C/m7G‐related regulators on the prognosis and immune microenvironment of glioma by integrated analysis of scRNA‐seq and bulk RNA‐seq data
Authors: Yang Longkun , Huang Zhicong , Deng Ying , Zhang Xing , Lv Zhonghua , Huang Hao , Sun Qian , Liu Hui , Liang Hongsheng , He Baochang , Hu Fulan 
Published: February 2024

AbstractBackgroundProliferation, metabolism, tumor occurrence and development in gliomas are greatly influenced by RNA modifications. However, no research has integrated the four RNA methylation regulators of m6A, m1A, m5C and m7G in gliomas to analyze their relationship with glioma prognosis and intratumoral heterogeneity.MethodsBased on three in‐house single‐cell RNA‐sequencing (scRNA‐seq) data, the glioma heterogeneity and characteristics of m6A/m1A/m5C/m7G‐related regulators were elucidated. Based on publicly available bulk RNA‐sequencing (RNA‐seq) data, a risk‐score system for predicting the overall survival (OS) for gliomas was established by three machine learning methods and multivariate Cox regression analysis, and validated in an independent cohort.ResultsSeven cell types were identified in gliomas by three scRNA‐seq data, and 22 m6A/m1A/m5C/m7G‐related regulators among the marker genes of different cell subtypes were discovered. Three m6A/m1A/m5C/m7G‐related regulators were selected to construct prognostic risk‐score model, including EIFA, NSUN6 and TET1. The high‐risk patients showed higher immune checkpoint expression, higher tumor microenvironment scores, as well as higher tumor mutation burden and poorer prognosis compared with low‐risk patients. Additionally, the area under the curve values of the risk score and nomogram were 0.833 and 0.922 for 3 year survival and 0.759 and 0.885 for 5 year survival for gliomas. EIF3A was significantly highly expressed in glioma tissues in our in‐house RNA‐sequencing data (p < 0.05).ConclusionThese findings may contribute to further understanding of the role of m6A/m1A/m5C/m7G‐related regulators in gliomas, and provide novel and reliable biomarkers for gliomas prognosis and treatment.
Changes in bone marrow fibrosis during momelotinib or ruxolitinib therapy do not correlate with efficacy outcomes in patients with myelofibrosis
Authors: Oh Stephen T. , Verstovsek Srdan , Gupta Vikas , Platzbecker Uwe , Devos Timothy , Kiladjian Jean‐Jacques , McLornan Donal P. , Perkins Andrew , Fox Maria Laura , McMullin Mary Frances , Mead Adam J. , Egyed Miklos , Mayer Jiri , Sacha Tomasz , Kawashima Jun , Huang Mei , Strouse Bryan , Mesa Ruben 
Published: February 2024

AbstractBone marrow fibrosis (BMF) is a pathological feature of myelofibrosis, with higher grades associated with poor prognosis. Limited data exist on the association between outcomes and BMF changes. We present BMF data from Janus kinase (JAK) inhibitor–naive patients from SIMPLIFY‐1 (NCT01969838), a double‐blind, randomized, phase 3 study of momelotinib vs ruxolitinib. Baseline and week 24 bone marrow biopsies were graded from 0 to 3 as per World Health Organization criteria. Other assessments included Total Symptom Score, spleen volume, transfusion independence status, and hemoglobin levels. Paired samples were available from 144 and 160 patients randomized to momelotinib and ruxolitinib. With momelotinib and ruxolitinib, transfusion independence was achieved by 87% and 44% of patients with BMF improvement of ≥1 grade and 76% and 56% of those with stable/worsening BMF; there was no association between BMF changes and transfusion independence for either arm (momelotinib, p = .350; ruxolitinib, p = .096). Regardless of BMF changes, hemoglobin levels also generally increased on momelotinib but decreased on ruxolitinib. In addition, no associations between BMF changes and spleen (momelotinib, p = .126; ruxolitinib, p = .407)/symptom (momelotinib, p = .617; ruxolitinib, p = .833) outcomes were noted, and no improvement in overall survival was observed with ≥1‐grade BMF improvement (momelotinib, p = .395; ruxolitinib, p = .407). These data suggest that the anemia benefit of momelotinib is not linked to BMF changes, and question the use of BMF assessment as a surrogate marker for clinical benefit with JAK inhibitors.
Role of lymphatic endothelium specific hyaluronan receptor 1 in virus infection and associated diseases
Authors: Dai Lu , Qin Zhiqiang 
Published: February 2024

AbstractLymphatic vessel endothelial hyaluronan receptor 1 (LYVE‐1) serves as a prominent marker for lymphatic endothelial cells (LECs) and is pivotal in the process of lymphangiogenesis, a critical factor in cancer development and metastasis. Overexpression of LYVE‐1 has been observed in various cancers, where it is recognized as an adverse prognostic indicator. Targeting LYVE‐1 has demonstrated inhibitory effects on tumor cell proliferation, migration, and the formation of lymph node metastases both in vitro and in vivo. While extensive research has focused on the role of LYVE‐1 in cancer cells, its involvement in virus infection and associated diseases remains largely unexplored. This review consolidates recent findings regarding the expression of LYVE‐1 and its functions in lymphangiogenesis during various viral infections and the development of related diseases, with a particular emphasis on Kaposi's sarcoma herpesvirus. Despite the limited available data, it is evident that further studies are essential to comprehensively understand the contribution of LYVE‐1 to viral pathogenesis and oncogenesis.
Prevalence and significance of HPV DNA detection below the clinical threshold of the commercial kit Alinity m HR‐HPV assay (Abbott)
Authors: Node Juliette , Dor Fanny , Puget Line , Ramanah Rajeev , Cot Sophie , Bibeau Frédéric , Di Domizio Nolwen , Prétet Jean‐Luc , Lepiller Quentin 
Published: February 2024

AbstractThe positive clinical threshold of human papillomavirus (HPV) tests validated for primary cervical cancer screening (CCS) is designed to offer an optimal balance between clinical sensitivity and specificity. However, there may be a gap between the analytical sensitivity of the test and the positive clinical threshold, referred to here as the “gray‐zone.” This study aims to determine the prevalence and significance of HPV results obtained in the gray‐zone in routine practice. Cervical samples obtained in our institution for CCS over a 22‐month‐period were tested with the Alinity m HR‐HPV Assay (Abbott). Clinical and biological data, including cytological results and patients' HPV history were collected. Of the 6101 samples collected, 1.7% had an HPV result in the gray‐zone (102 patients). The proportion of gray‐zone results varied according to HPV genotype, reaching 11.8% of samples with detectable HPV DNA in the case of HPV31/33/52/58 genotypes. Reflex cytologies showed no abnormalities or Atypical Squamous Cells of Undetermined Significance results in 74.6% and 17.9% of cases, respectively. A previous or subsequent HPV‐positive result with a (possibly) identical genotype was observed in 58% and 38% of cases, respectively. Two women with a history of persistent HPV detection had a CIN2+ lesion 1 year after the gray‐zone result. In conclusion, the proportion of HPV results in the gray‐zone varies according to genotype. No cytological abnormality is observed in the majority of cases, but a few rare patients with a history of persistent HPV infection should be closely monitored even if the HPV result is transiently located in the gray‐zone.
Postural orthostatic tachycardia syndrome and other autonomic dysfunctions following COVID‐19: Incidence, characteristics, and associated factors
Authors: Tanking Chonthicha , Lakkananurak Chanokporn , Srisakvarakul Chaval , Jitpreeda Amaraluk , Threechod Kullisara , Sukitpunyaroj Damrong 
Published: February 2024

AbstractBackgroundLong‐COVID syndrome has become a new health concern. Many major clinical centers have experienced more patients with symptoms suggestive of autonomic dysfunction, especially postural orthostatic tachycardia syndrome (POTS) following COVID‐19. However, there is a lack of information regarding the incidence and associated factors in Asian population.MethodsA retro‐prospective study was conducted to evaluate patients with symptoms suggestive of POTS or other autonomic dysfunctions. These symptoms last at least 3 months after PCR‐proven COVID‐19. Exclusion criteria were age under 18 years old, pregnancy, and pre‐COVID‐19 autonomic dysfunction symptoms. Patients with a symptom severity score greater than two were assessed with blood tests, 24‐h Holter, 24‐h ambulatory blood pressure, echocardiogram, and head‐up tilt table (HUTT).ResultsSeven hundred ninety‐three patients were interviewed at 146 ± 37 days after COVID‐19. The majority of patients were middle‐aged females (53%). Of those, 15 patients had the symptom severity score greater than 2. Out of those 15 patients, 12 had positive HUTT (1 demonstrating POTS, 10 neurocardiogenic syncope, and 1 orthostatic hypotension). Among those with positive HUTT patients, C‐reactive protein (CRP) was significantly higher (OR 1.01; p‐value 0.041). Fatigue and dyspnea on exertion were the two most complaint symptoms.ConclusionsThis study shows the incidence of autonomic dysfunction and POTS is 1.5% (12/793) and 0.1% POTS (1/793), respectively, in a primary care setting (among general post‐COVID‐19 patients). The most common symptoms for these patients were fatigue and dyspnea.
Characterization of dental calculus in the South American Quaternary proboscidean Notiomastodon platensis (Ameghino, 1888)
Authors: Fonseca De Paiva Ana Clara , Alves‐Silva Laís , De Souza Barbosa Fernando Henrique 
Published: February 2024

ABSTRACTDental calculus, or tartar, is a mineralized biofilm that develops on the teeth, and is often observed on the teeth of the South American proboscidean Notiomastodon platensis. This structure can provide details on paleoecological, paleoenvironmental and paleopathological aspects of a species. However, characterization of tartar in N. platensis is lacking. The aim of this study was to provide a macromorphological characterization of tartar on N. platensis molars, and evaluate its prevalence, distribution patterns and severity. A total of 117 molariforms from Brazilian Quaternary fossiliferous assemblages were analyzed. Thirty‐five (29.9%) molars presented tartar. The majority displayed moderate severity. Calculus was present at least on one inteloph(id) of each molar, and prevailed on the posterior region. It predominated on lower and third molars. Adult individuals showed a predominance compared to juveniles. These patterns in N. platensis are probably due to the susceptible oral environment, molar morphology, mature age and tooth replacement. These results suggest that N. platensis and probably other proboscideans were relatively susceptible to tartar development.