bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tannic acid alleviates ETEC K88‐induced intestinal damage through regulating the p62‐keap1‐Nrf2 and TLR4‐NF‐κB‐NLRP3 pathway in IPEC‐J2 cells
Authors: Liu Wenhui , Guo Kangkang 
Published: January 2024

AbstractBACKGROUNDTannic acid (TA), a naturally occurring polyphenol, has shown diverse potential in preventing intestinal damage in piglet diarrhea induced by Enterotoxigenic Escherichia coli (ETEC) K88. However, the protective effect of TA on ETEC k88 infection‐induced post‐weaning diarrhea and its potential mechanism has not been well elucidated. Therefore, an animal trial was carried out to investigate the effects of dietary supplementation with TA on the intestinal diarrhea of weaned piglets challenged with ETEC K88. In addition, porcine intestinal epithelial cells were used as an in vitro model to explore the mechanism through which TA alleviates intestinal oxidative damage and inflammation.RESULTSThe results indicated that TA supplementation (2 and 4 g kg−1) reduced diarrhea rate, enzyme activity (diamine oxidase [DAO] and Malondialdehyde [MAD]) and serum inflammatory cytokines concentration (TNF‐α and IL‐1β) (P < 0.05) compared to the Infection group (IG), group in vivo. In vitro, TA treatment effectively alleviated ETEC‐induced cytotoxicity, increased the expression of ZO‐1, occludin and claudin‐1 at both mRNA and protein levels. Moreover, TA pre‐treatment increased the activity of antioxidant enzymes (such as T‐SOD) and decreased serum cytokine levels (TNF‐α and IL‐1β). Furthermore, TA increased cellular antioxidant capacity by activating the Nrf2 signaling pathway and decreased inflammatory response by down‐regulating the expression of TLR4, MyD88, NF‐kB and NLRP3.CONCLUSIONThe present study showed that TA reduced the diarrhea rate of weaned piglets by restoring the intestinal mucosal mechanical barrier function, alleviating oxidative stress and inflammation. The underlying mechanism was achieved by modulating the p62‐keap1‐Nrf2 and TLR4‐NF‐κB‐NLRP3 pathway. © 2024 Society of Chemical Industry.
Cut me some slack! How perceptions of financial slack influence pain of payment
Authors: Pomerance Justin , Reinholtz Nicholas 
Published: January 2024

AbstractConsumers often experience pain of payment, a tug of negative affect that holds back their spending. While the literature has long viewed pain of payment as self‐regulatory in nature, it has left the dynamics of self‐regulation that lead to the pain of paying largely unaddressed. In self‐regulation, affect arises when people move away from a goal they hold. Thus, understanding the specific goals that people consider when making a payment can help us better predict when pain of payment will arise. We propose that people have a goal to maintain financial slack, and that violating this goal contributes to pain of payment. Thus, people experience more pain of payment when the goal to maintain financial slack is stronger or when it is particularly salient that a purchase entails losing financial slack. Critically, subjective changes in financial slack are not equivalent to objective changes in wealth, altering pain of payment for economically equivalent trades. This research contributes to the existing literature by identifying a novel antecedent to the pain of payment. It additionally expands our understanding of people's preferences between payment systems. Finally, it offers guidance to practitioners who wish to minimize pain of payment among their consumers.
MaDDOSY (Mass Determination Diffusion Ordered Spectroscopy) using an 80 MHz Bench Top NMR for the Rapid Determination of Polymer and Macromolecular Molecular Weight
Authors: Tooley Owen , Pointer William , Radmall Rowan , Hall Mia , Beyer Valentin , Stakem Kieran , Swift Thomas , Town James , Junkers Tanja , Wilson Paul , Lester Daniel , Haddleton David 
Published: January 2024

AbstractMeasurement of molecular weight is an integral part of macromolecular and polymer characterization which usually has limitations. Herein, this article presents the use of a bench‐top 80 MHz Nuclear Magnetic Resonance (NMR) spectrometer for diffusion‐ordered spectroscopy as a practical and rapid approach for the determination of molecular weight/size using a novel solvent and polymer‐independent universal calibration.
Surgical management of bladder outlet obstruction due to functional and anatomical etiologies in women
Authors: Dequirez Pierre‐Luc , Wasserman Meredith C. , Brucker Benjamin M. 
Published: January 2024

AbstractIntroductionBladder outlet obstruction (BOO) in women includes functional and anatomic etiologies. Primary bladder neck obstruction (PBNO), Fowler's syndrome (FS), and dysfunctional voiding (DV) are some examples of functional obstructions, whereas pelvic organ prolapse (POP), periurethral masses, and intragenic causes are some of the anatomic causes.MethodsThis literature review describes the etiologies of female BOO, unique aspects of the workup and diagnosis, and the data for the standard surgical treatments and newer surgical techniques to treat women. Urethral stenosis and sling‐related obstruction are treated in the other articles of this series. Where possible the focus is the efficacy and outcomes.ResultsTreatment of PBNO using a transurethral incision of the bladder neck and injection of botulinum toxin in the bladder neck decreases the BOO. After the failure of conservative approaches, sacral neuromodulation (SNM) is effective for FS, while DV may benefit from SNM or botulinum toxin injections. Concerning POP, most surgeries have been reported to significantly improve a pre‐existent BOO but the level of evidence is low. Benign urethral and periurethral masses may provoke BOO, and surgical excision usually resolves this condition.ConclusionAlthough most surgical treatments of BOO for functional and benign anatomical etiologies in women seem to be effective, data are scarce even for more common conditions like POP. Further studies are required to give better advice on the choice of surgical technique for these patients.
Lifelong LUTS: Understanding the bladder's role and implications across transition phases, a comprehensive review
Authors: Kheir George Bou , Verbakel Irina , Wyndaele Michel , Monaghan Thomas F. , Sinha Sanjay , Larsen Tove Holm , Van Laecke Erik , Birder Lori , Hervé François , Everaert Karel 
Published: January 2024

AbstractIntroductionLower urinary tract symptoms (LUTSs) are a diverse array of urinary and pelvic dysfunctions that can emerge from childhood, extend through adulthood, and persist into older age. This narrative review aims to provide a comprehensive perspective on the continuum of LUTS and shed light on the underlying mechanisms and clinical implications that span across the lower urinary tract.MethodsA panel of five experts from Belgium, the Netherlands, India, Denmark, and the United States participated in an intensive research to explore and pinpoint existing insights into the lifelong concept of LUTS, particularly at the pelvic level. The experts reviewed the existing literature and held a webinar to discuss their findings.ResultsChildhood LUTS can persist, resolve, or progress into bladder underactivity, dysfunctional voiding, or pain syndromes. The Lifelong character can be explained by pelvic organ cross‐talk facilitated through complex neurological and nonneurological interactions. At the molecular level, the role of vasopressin receptors in the bladder's modulation and their potential relevance to therapeutic strategies for LUTS are explored. Frailty emerges as a parallel concept to lifelong LUTS, with a complex and synergistic relationship. Frailty, not solely an age‐related condition, accentuates LUTS severity with insufficient evidence regarding the effectiveness and safety profile of the available therapeutic modalities.ConclusionUnderstanding lifelong LUTSs offers insights into genetic, anatomical, neurological, and molecular mechanisms. Further research could identify predictive biomarkers, elucidate the role of clinically translatable elements in pelvic cross‐talk, and uncover molecular signatures for personalized management.
Oral health and nonmolar dental attrition in the Siwa‐period individuals from the Bronze Age Mogou cemetery, Northwest China
Authors: Monroe Shannon , Dittmar Jenna M. , Berger Elizabeth , Dautartas Angela , Mao Ruilin , Wang Hui , Yeh Ivy Hui‐Yuan 
Published: January 2024

AbstractDental data can reveal evidence for a past population's oral health, nutrition, and certain cultural activities. This study aims to explore oral health and dental attrition during the late Bronze Age in order to explore health outcomes in different subgroups as well as aspects of foodways and changes in subsistence strategies during the second millennium BCE in northwest China. To do this, the skeletal remains of adult individuals associated with the Siwa material culture (1400–1100 BC) from the Mogou site (n = 28) were macroscopically assessed and compared with previously published data derived from a subsample of individuals associated with Qijia period material culture complex (1750–1400 BC) from the same site. The results show that the Siwa‐period population experienced a high frequency of carious lesions and antemortem tooth loss associated with advanced attrition (of both molars and nonmolar teeth), which did not vary significantly by sex. Females had a higher prevalence of carious lesions and antemortem tooth loss than did males, while males had a higher prevalence of dental calculus. These male/female health outcomes are also attested during the earlier Qijia period at the Mogou site. The Siwa period differs from the Qijia in that females experienced slightly worse attrition than their predecessors. Overall, oral health does not diverge significantly between the Qijia and Siwa periods, suggesting that the factors that contributed to oral health including dietary practices may have persisted diachronically for individuals buried at this site.
Cross‐cultural adaptation, validity, and reliability of the Turkish version of the Oral Behaviours Checklist
Authors: Kaynak Besime Ahu , Aktaş Arda , Tüfek Muhammed Taha , Taş Serkan 
Published: January 2024

AbstractAimDetermining the occurrence of oral parafunctional behaviors is essential for the clinical management of temporomandibular disorders (TMDs). For this reason, the evaluation of parafunctional behaviors in patients with TMDs is highly important.MethodsThe purpose of the present study was to investigate the validity and reliability of the Turkish version of the Oral Behaviours Checklist (OBC‐T) in patients with TMDs. To determine the reliability of the questionnaire, it was applied in 142 patients with TMDs twice at a 2‐week interval. For concurrent validity of the OBC, Spearman correlation analysis was performed with Fonseca Anamnestic Index (FAI), Patient Health Questionnaire‐9 (PHQ‐9), Jaw Function Limitation Scale‐20 (JFLS‐20), and Tampa Scale for Kinesiophobia for TMDs (TSK‐TMDs).ResultsItems had good to excellent test–retest reliability. The OBC‐T score was correlated with FAI, JFLS‐20, PHQ‐9, and TSK‐TMDs scores.ConclusionThese results provided important evidence that the OBC‐T can be used to evaluate parafunctional behaviors in individuals with TMDs in Turkish‐speaking populations.
Chemokine profiling of melanoma–macrophage crosstalk identifies CCL8 and CCL15 as prognostic factors in cutaneous melanoma
Authors: Barrio‐Alonso Celia , Nieto‐Valle Alicia , García‐Martínez Elena , Gutiérrez‐Seijo Alba , Parra‐Blanco Verónica , Márquez‐Rodas Iván , Avilés‐Izquierdo José Antonio , Sánchez‐Mateos Paloma , Samaniego Rafael 
Published: January 2024

AbstractDuring cancer evolution, tumor cells attract and dynamically interact with monocytes/macrophages. To find biomarkers of disease progression in human melanoma, we used unbiased RNA sequencing and secretome analyses of tumor–macrophage co‐cultures. Pathway analysis of genes differentially modulated in human macrophages exposed to melanoma cells revealed a general upregulation of inflammatory hallmark gene sets, particularly chemokines. A selective group of chemokines, including CCL8, CCL15, and CCL20, was actively secreted upon melanoma–macrophage co‐culture. Because we previously described the role of CCL20 in melanoma, we focused our study on CCL8 and CCL15 and confirmed that in vitro both chemokines contributed to melanoma survival, proliferation, and 3D invasion through CCR1 signaling. In vivo, both chemokines enhanced primary tumor growth, spontaneous lung metastasis, and circulating tumor cell survival and lung colonization in mouse xenograft models. Finally, we explored the clinical significance of CCL8 and CCL15 expression in human skin melanoma, screening a collection of 67 primary melanoma samples, using multicolor fluorescence and quantitative image analysis of chemokine–chemokine receptor content at the single‐cell level. Primary skin melanomas displayed high CCR1 expression, but there was no difference in its level of expression between metastatic and nonmetastatic cases. By contrast, comparative analysis of these two clinically divergent groups showed a highly significant difference in the cancer cell content of CCL8 (p = 0.025) and CCL15 (p < 0.0001). Kaplan–Meier curves showed that a high content of CCL8 or CCL15 in cancer cells correlated with shorter disease‐free and overall survival (log‐rank test, p < 0.001). Our results highlight the role of CCL8 and CCL15, which are highly induced by melanoma–macrophage interactions in biologically aggressive primary melanomas and could be clinically applicable biomarkers for patient profiling. © 2024 The Pathological Society of Great Britain and Ireland.
Prevention of potential‐induced degradation using a moisture barrier in crystalline silicon photovoltaic modules
Authors: Lee Solhee , Lee Kyung Dong , Bae Soohyun , Kang Yoonmook , Kim Donghwan , Lee Hae‐Seok 
Published: January 2024

AbstractAs photovoltaic (PV) modules are exposed to high temperatures and humidity over time, they generate leakage current, which leads to potential‐induced degradation (PID) and lower power output. In silicon, Cu(In,Ga)(Se,S)2 (CIGS) thin film and perovskite solar cells, PID has been shown to be driven by the presence of Na in the module glass. PID stability is crucial for the commercialization of such solar modules. This study aims to confirm the leaching phenomenon of Na in soda–lime module glass and study the use of polytetrafluoroethylene (PTFE) as a moisture barrier to prevent PID. By water immersion and exposure to different temperature and humidity conditions, we exhibited Na leaching in soda–lime glass. Moreover, we demonstrate the use of an anti‐PID moisture barrier made of PTFE, which was deposited using kinetic spraying between the cover glass and encapsulant in the solar module. The thickness of the moisture barrier was controlled by adjusting the deposition rate, and the PID characteristics were evaluated by manufacturing solar modules for different barrier thicknesses. Light current–voltage (LIV), dark current–voltage (DIV), and electroluminescence (EL) measurements confirmed that the PTFE moisture barrier effectively inhibits the degradation of solar cells. This study provides further insights into the Na leaching phenomenon and PID mechanism in PV modules and contributes to the design and development of more stable solar cells.
Physiologic stress in the classroom: Does teacher's cortisol expression influence children's afternoon rise in cortisol at childcare?
Authors: Schlueter Lisa J. , McGee Andrew B. , Link Tasha , Badanes Lisa S. , Dmitrieva Julia , Watamura Sarah E. 
Published: January 2024

AbstractExtant literature has demonstrated that children's diurnal stress physiology often looks different on childcare versus home days. Specifically, children experience a rise in cortisol, rather than a decline, over the day while in full‐time care. Additionally, temperamental fit within classroom environment may influence both child and teacher cortisol and outcomes. The current study investigated whether teachers' cortisol levels (n = 11) were associated with child cortisol levels (n = 71, mean age = 4.18 ± 0.81, 51% female) in the classroom while taking into account temperament, child sex assigned at birth, and classroom quality. Cortisol samples were collected on consecutive days at home and childcare for children, and at childcare for teachers. We hypothesized that (1) teacher cortisol will predict child afternoon cortisol, (2) that a portion of the variance in this relationship will be accounted for by classroom quality. Using a cross‐sectional design, children in this sample had higher cortisol values in the afternoon while at childcare than at similar times of day at home. Teachers demonstrated a typical diurnal decline across the day but variation in total cortisol output at childcare. Two distinct predictors of afternoon cortisol levels were identified in children attending childcare: child age and mean teacher cortisol levels across the day, while negative affect and classroom quality were not significant. Younger children and children whose teachers had higher mean cortisol levels were more likely to have high afternoon cortisol at childcare. Higher diurnal cortisol values for teachers may indicate a higher stress environment for both teachers and children. These preliminary findings suggest that teacher stress may not only have implications for children in terms of teacher burnout, high staff turnover, and learning, but possibly also for children's health and well‐being. Implications for research and policy, as well as limitations and strengths, are discussed.
A new feature of the laboratory model plant Nicotiana benthamiana: Dead‐end trap for sustainable field pest control
Authors: Han Wen‐Hao , Wang Jun‐Xia , Zhang Feng‐Bin , Ji Shun‐Xia , Zhong Yu‐Wei , Liu Yin‐Quan , Liu Shu‐Sheng , Wang Xiao‐Wei 
Published: January 2024

Societal Impact StatementConventional pest management measures release large amounts of chemical or plastic pollutants into the environment. Nicotiana benthamiana, a commonly employed laboratory model plant, demonstrated remarkable lethality and attractiveness towards several important Hemiptera and Thysanoptera insect pests. Field trials showed that the N. benthamiana plants can be used as a dead‐end trap to successfully control these pests, comparable to commercial plastic sticky traps. Notably, the N. benthamiana in the field grew well without negatively impacting crop growth, natural enemies, and pollinators. This study unearths a new benefit of N. benthamiana and develops an easy‐to‐apply and sustainable strategy for field pest control.Summary

Hemiptera and Thysanoptera insects pose persistent threats to agricultural production. Conventional management strategies involve the release of chemical or plastic agents, causing adverse environmental and global health issues. Notably, Nicotiana benthamiana, a globally utilized model plant, exhibits remarkable lethal effects and attraction towards these pests.
In this study, we explored the potential of using N. benthamiana for Hemiptera and Thysanoptera pest control in the laboratory and field. Through net cover and three field assays over 2 years, we demonstrated the efficacy and benefits of using N. benthamiana as a field‐deployed pest control dead‐end trap.
N. benthamiana demonstrated nearly 100% lethality to whiteflies, aphids, and thrips, with emitted volatiles attracting these insects. Field trials showed that potted and planted N. benthamiana blocks and traps whiteflies and thrips from several Solanaceae and Cucurbitaceae crops effectively, comparable to common commercial yellow and blue sticky boards. Moreover, N. benthamiana in the field exhibited robust growth in commercial greenhouses without negatively impacting crop growth, natural enemies, and pollinators.
Our study introduces an innovative, easily implementable, and sustainable approach for controlling Hemiptera and Thysanoptera pests. Moreover, it unveils the novel utility of N. benthamiana in field‐based pest management.
Precision radiotherapy for nasopharyngeal carcinoma
Authors: Zhang Zhenyu , Chen Xiangzhou , Yuan Taize 
Published: January 2024

AbstractNasopharyngeal carcinoma(NPC) occurs frequently in Southern China, and radiotherapy is the main treatment method. At present, intensity‐modulated radiotherapy is widely used, which has improved efficacy in patients with NPC and reduced toxicity and side effects. Recently, helical tomography radiotherapy, proton radiotherapy, carbon particle radiotherapy, and other radiotherapy techniques have been used for the clinical treatment of NPC. Individualized nasopharyngeal cancer targets have also been explored. This paper reviews the research progress in radiotherapy techniques and target volume for NPC
III. Geometrical framework for thinking about globular proteins: Turns in proteins
Authors: Škrbić Tatjana , Giacometti Achille , Hoang Trinh X. , Maritan Amos , Banavar Jayanth R. 
Published: January 2024

AbstractWe have shown recently that the notion of poking pairwise interactions along a chain provides a unifying framework for understanding the formation of both secondary and the tertiary protein structure based on symmetry and geometry. α‐helices and β‐sheets are found to be special geometries that have systematic poking contacts in a repetitive manner with the contacts being local along the α‐helix and non‐local along a pair of adjacent strands within a β‐sheet. Pairwise poking interactions also govern tertiary structure formation, but they are weaker and there are no special geometrical constraints as in secondary structure formation. Here we demonstrate that protein turns, the most prevalent non‐repetitive structural element in proteins, are instances of local (as in α‐helices) and isolated (non‐repetitive) poking pairwise contacts for which the geometrical constraints are partially relaxed. This simple and purely geometrical definition of protein turns (also sometimes known as reverse turns, β‐turns, β‐bends, hairpin bends, 310 bends, kinks, widgets, etc.) provides a simple framework for unifying them. We present the results of a systematic analysis and identify their structural classes as well as their respective amino acid preferences.