bluuebunny/crossref_metadata_2025_split huggingface dataset:
An LC–MS/MS assay for estimating conivaptan in human plasma
Authors: Cui Yanli , Wu Feng 
Published: February 2025

AbstractWe developed and validated a liquid chromatography–mass spectrometry (LC–MS/MS) method for detecting conivaptan, an arginine vasopressin receptor blocker, in human plasma to facilitate future pharmacokinetic studies. After acetonitrile protein precipitation, analytes were separated on a Grace Alltima HP C18 reverse-phase column (5 μm, 2.1 × 50 mm), and gradient elution was performed using 0.1% formic acid and 5 mM ammonium formate in acetonitrile–water (1:9) and acetonitrile–water (9:1) over a 4-min run time. Positive-mode electrospray ionization and multiple reaction monitoring were applied. The assay was linear over the concentration range of 1–500 ng mL−1, with confirmed accuracy (intra-batch, 0.5–1.1%) and precision (intra-batch relative standard deviation [RSD], ≤5.7%; inter-batch RSD, ≤6.8%). Extraction recovery varied between 94.2–100.5%, and the matrix effect was 94.9–97.0%. To establish the stability, we examined plasma for 17 h at room temperature, 21 h at 2–8 °C, 57 days at −70 °C for three freeze-thaw cycles, and after processing for 52 h in the autosampler. We detected acceptable stability under all examined conditions. Hemolysis, hyperlipidemia, and dilution integrity satisfied the validation criteria. The method developed in this current study will help accurately quantify conivaptan in human plasma and define its clinical pharmacokinetics in the future.
Overcoming Barriers to AI Implementation in University Libraries: A Developing Country Perspective
Authors: Akinola Samson Adesina 
Published: February 2025

This paper explores the use of artificial intelligence (AI) as an enabler for effective library services in universities in developing countries in the contemporary information age, focusing on its use in library functions, librarians' attitudes, readiness, challenges, and recommendations for effective implementation. The paper shows that AI has the potential to enhance library services and improve user experiences. It also assures that librarians play a crucial role in the implementation and use of AI, and their attitudes towards AI vary. Some librarians are excited about the possibilities AI offers, while others are skeptical or hesitant about its implementation in libraries. However, the paper concludes that librarians in developing countries express a readiness to adopt AI in university libraries. Despite this readiness, there are several challenges in implementing and using AI in university libraries in developing countries, including limited resources, lack of technical expertise, and concerns regarding privacy and data protection. To overcome these challenges and ensure effective use of AI, the paper recommends appropriate tools such embracing AI technologies like chatbots, RFID, and robotics for top-level service delivery, as well as leveraging AI to connect with remote users and regain relevance within the user base.
ANTIBACTERIAL EFFECTIVENESS OF BIODEGRADABLE MEMBRANES CONTAINING TIGECYCLINE IN AN IN VIVO EXPERIMENT
Authors: Kudryavtseva Yulia A. , Kanonykina Anastasia Yu. , Efremova Natalia A. 
Published: February 2025

HighlightsAbdominal surgeries are complicated by an infectious process, the development of which significantly aggravates the underlying disease, lengthens the patient’s stay in the hospital, increases the cost of treatment, causes deaths and negatively affects the recovery time of operated patients. In order to prevent the formation of adhesions and the development of infection, it is promising to use anti-adhesion membranes that have their own antibacterial activity. Aim. To study the antibacterial activity of biodegradable membranes containing tigecycline upon implantation into an infected wound in a laboratory animal model.Methods. We used the following composition of biodegradable polymers for the manufacture of membranes: copolymer polylactide-co-glycolide (50:50) Mm 20-30 KDa and polylactide-co-glycolide (85:15). We dissolved the polymers were in 1,1,1,3,3,3-Hexafluoroisopropanol, and to impart antibacterial properties to the membrane, we added the antibiotic tigecycline to the membrane composition, and assessed the optimal concentration of the antibiotic. We prepared the membranes using electrospinning. To evaluate the antibacterial effectiveness of membranes when implanted into a wound infected with Staphylococcus aureus we used an animal model (rat).Results. The prepared membranes containing tigecycline at a concentration of 0.5 mg/mL solution exerted maximum antibacterial effect. When implanted into an infected wound in laboratory animals, membranes containing tigecycline effectively suppressed the infectious process. By the 14th day of follow-up, we noted a complete healing of the wound, the absence of an inflammatory reaction, moreover we histologically confirmed an active infectious process.Conclusion. Biodegradable polymer membranes containing tigecycline as an antibacterial component effectively suppress the infectious process in an animal experiment.
RAUCHFUSS-KISSEL SYNDROME IN A CHILD WITH CONGENITAL HEART DEFECT. A CLINICAL CASE
Authors: Rumiantseva Alexandra A. , Perevalova Natalia G. , Kokov Alexandr N. , Sizova Irina N. 
Published: February 2025

Highlights The review describes a rare clinical case of isolated left ventricular apical hypoplasia with atrial septal defect. AbstractIsolated left ventricular apical hypoplasia is characterized by impaired left ventricle. The frequency of the disease is 0.5-0.75% and it can be easily detected on echocardiography. The disease is difficult to diagnose due to absence of symptoms, therefore timely diagnosis and treatment can prevent any complications and improve the quality of life.
ASSOCIATION BETWEEN BRAIN NATRIURETIC PEPTIDE LEVELS AND MARKERS OF THE SEVERITY OF RESISTANT ARTERIAL HYPERTENSION
Authors: Panarina Anna Ya. , Nikolaev Konstantin Yu. , Shilova Angelika V. , Lifshits Galina I. 
Published: February 2025

HighlightsThe complex role of the brain natriuretic peptide in the pathogenesis of arterial hypertension, as well as the successful use of the N-terminal fragment of the prohormone brain-type natriuretic peptide as a biomarker of cardiovascular diseases determine the relevance of this work. Aim. To study the association between brain natriuretic peptide levels (NT-proBNP) and markers of the severity of resistant arterial hypertension.Methods. The study included 40 patients with diagnosed resistant arterial hypertension (RAH) and 30 patients with AH. All patients underwent clinical examination and measurement of NT-proBNP levels before and after receiving standard antihypertensive therapy.Results. Before receiving standardized antihypertensive therapy (AHT), NT-proBNP levels in the group of patients with RAH and in the group of patients with non-resistant hypertension did not differ significantly (p = 0.48), but systolic BP was statistically significantly higher (p = 0.039) in the group of patients with RAH. RAH revealed a direct association between NT-proBNP and sodium (r = 0,38; p = 0.01), as well as a direct association between NT-proBNP and body mass index (r = 0,32; p = 0.02). Factor analysis revealed a relationship between NT-proBNP and age (p < 0.01), as well as a direct relationship between NT-proBNP and potassium (r = 0,29; p = 0.04). After receiving standardized AHT, NT-proBNP levels decreased in the RAH group and in the non-resistant AH group (p = 0.03; p = 0.0001), but the decrease was less pronounced in the RAH group. NT-proBNP levels after standardized AHT in the RAH group were higher (p < 0.0001) compared to the non-resistant AH group, and relatively high NT-proBNP levels were directly associated with the presence of RAH (p = 0.0025).Conclusion. The results of the study indicate the presence of an association between NT-proBNP levels and resistant arterial hypertension. Supposedly, this association determines the uncontrolled course of resistant arterial hypertension – desensitization of receptors that regulate vascular tone, lipid metabolism disorders and natriuresis dysfunction.
Renal Protective Effect of Resveratrol against Vincristine-Induced Oxidative Stress in Female Mice
Authors: Nasirzadeh Mohammadreza , Khayatnouri Mir Hadi , Taginasab Saeed , Navidi far Pouya , Navidi far Pouya , Bakhshi Mohammad , Tayefesattari Heidar 
Published: February 2025

Introduction: Vincristine (VIN) is a broad-spectrum anticancer drug used to treat various cancers. Resveratrol (Res) is a natural polyphenol found in numerous plant sources. Many studies have reported anti-inflammatory and antioxidative effects of resveratrol. In this study, the effect of resveratrol on kidney damage caused by vincristine in female mice has been investigated.
Methods: In this study, 36 female mice weighing 25-30 grams were randomly divided into four groups (n=9): 1) Control group, 2) Vin- group, 3) Vin-Res group and 4) Res group. The mice received vincristine at 3 mg/kg once a week for 4 weeks and resveratrol at 30 mg/kg daily for 28 days through gavage. At the end of the study, the fat peroxidation index (MDA), total antioxidant capacity (TAC), and the activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) in kidney tissue were measured. Data were presented as mean ± standard deviation, and the significant differences among groups were analyzed using SPSS16, one-way ANOVA, and Tukey's post hoc test.
Results: The findings indicated that in the vincristine group, levels serum of urea, creatinine, and MDA in kidney tissue were increased significantly compared to the control group (P=0.001).the TAC level and the activity level of GPX (P=0.001) and SOD (P=0.009) enzymes in the kidney tissue were significantly decreased in the vincristine group when compared to the control group (P=0.001).
Conclusion: The results of this study showed that the protective effects of resveratrol were probably attributed to its antioxidant properties and that it could reduce the kidney damage induced by vincristine.
Effect of flipped classroom on student perceptions, academic scores and study practices
Authors: Wali Othman , Vanka Shanthi , Vanka Amit 
Published: February 2025

Aim. To evaluate the educational impact of two flipped classroom models.
Material and methods. Traditional lectures were followed by Flipped classroom (FC) with face to face(F2F) session (cohort of 2019-20) and online (cohort 2020-21). Students’ perceptions were collected by questionnaire after lectures and FC. Historic academic scores on selected topic were compared scores after introduction of FC. The learning management system(LMS) was used to monitor student usage of learning resources.
Results. Students perceptions for cohort 2019-20 were significantly higher(p>0.05) for FC(F2F) compared to lecture. Students’ perceptions for cohort 2020-21 were also significantly higher for FC(online) compared to online lecture. Academic scores did not show significant difference between lecture and FC. Increased hits on LMS for online resources were associated with FC or summative assessment.
Conclusions. Students were overwhelmingly positive on FC for both: with F2F and online , and academic scores were similar to lecture. FC can possibly improve student study habits but needs further research.
Intimate Partner Violence, Perempuan Korban Kekerasan Dalam Masa Pacaran : Sebuah Laporan Kasus
Authors: Fatdiansyah Endra , Utomo Raden Panji Uva , Rohmah Intarniati Nur 
Published: February 2025

Latar belakang : Kekerasan pada perempuan terutama dalam konteks Intimate Partner Violence (IPV) (kekerasan hubungan intim), merupakan suatu masalah serius dan posisi perempuan sebagai pihak yang rentan (vulnerable) sering menjadi target dari kekerasan yang dilakukan oleh pasangan. Sistem hukum di indonesia belum mengenal pola hubungan berdasarkan kekuasaan pada IPV, namun pengetahuan ahli forensik dalam hal tersebut dapat memberi petunjuk dalam proses penegakan hukum dan intervensi dalam membantu korban.
Presentasi Kasus : seorang perempuan berusia 29 tahun, mengaku dianiaya oleh pacar, mengalami luka memar pada kepala, pipi, leher, punggung, pinggang, anggota gerak atas; luka lecet pada pipi, anggota gerak atas, dan anggota gerak bawah kiri.
Pembahasan : Laporan kasus ini berfokus pada kasus IPV pada perempuan di posisi rentan dan melihat kaitannya Intimate Partner Violence (kekerasan pasangan intim). Tujuan dari tulisan ini adalah untuk mengkaji kasus IPV berdasarkan posisi korban dan pelaku dapat diterapkan pada kasus untuk perempuan rentan dan memberikan petunjuk mengenai intervensi tatalaksana kasus. Korban mengalami pola hubungan Physical Violence.
Simpulan : Sistem hukum di Indonesia belum mengenal pola hubungan berdasarkan kekuasaan pada IPV, namun pengetahuan ahli forensik dalam hal tersebut dapat memberi petunjuk dalam proses penegakan hukum dan intervensi dalam membantu korban.
Kata kunci: kekerasan pasangan intim, perlindungan hukum, kelompok rentan
PEMAHAMAN ISU GENDER MELALUI PEMBELAJARAN BERDIFERENSIASI BERBANTUAN LEARNER GENERATED DIGITAL MEDIA (LGDM )
Authors: Gusti Ririn , Putra Ari 
Published: February 2025

Penelitian ini bertujuan untuk mengeksplorasi penggunaan Learner Generated Digital Media (LGDM ) dalam meningkatkan pemahaman mengenai isu gender dalam mata kuliah Gender dalam Pendidikan. Menggunakan pendekatan kualitatif. Data dikumpulkan melalui wawancara mendalam dengan pembelajar, observasi langsung selama sesi pembelajaran, dan analisis terhadap dokumen digital yang dihasilkan pembelajar. Temuan penelitian mengungkapkan bahwa LGDM pembelajar dapat berpartisipasi lebih aktif dan terlibat secara mendalam dalam proses pembelajaran. Penggunaan media digital yang dihasilkan sendiri oleh pembelajar meningkatkan pemahaman terhadap isu gender secara signifikan. Selain itu, LGDM juga membantu peserta pembelajaran dalam mengembangkan keterampilan kritis dan reflektif yang lebih baik melalui produksi dan analisis media digital yang berkaitan dengan isu gender. Penelitian ini menjadi solusi bahwa penerapan LGDM dalam pembelajaran dapat memperkaya pengalaman belajar dan memperdalam pemahaman peserta pembelajaran tentang isu gender, serta merekomendasikan integrasi metode ini dalam kurikulum pendidikan gender yang lebih luas.
WITHDRAWN: Insights into microbial contamination in paddy field ecology: matagenomic analysis of the abundances, diversity, and distribution of antibiotic resistance genes, virulence factors, and their potential bacterial hosts
Authors: Chen Yan , Chu Shanshan , Xu Zhuoqun , Zhou Jie , Li Keyu , Li Sisi , Zhang Yaru , Han Jianzhong , QU DAOFENG 
Published: February 2025

Abstract

The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference.
Resilience and its relation to Alexithymia and personality traits in Egyptian medical students: A Cross-Sectional Study
Authors: Azzam Hajer , Awad Kariem , Ata Ahmed H. , Elagouz Mennatullah , Nabih Mariam H. , ElKaffas Mahmoud E. , Hafiz Mohamed , Ibrahim Ibtihal M.A. 
Published: February 2025

Abstract

Background: Mental health issues among medical students are increasingly prevalent. Resilience, the ability to positively adapt in the face of adversity, is crucial for reducing psychological distress and enhancing overall well-being. About half of students in Egypt have low resilience. Alexithymia, the difficulty in identifying and describing emotions, alongside personality traits, influences resilience. This study aims to assess the level of resilience in medical students at Mansoura University and to explore the effects of alexithymia and personality traits on resilience.
Methods: A uni-centre descriptive cross-sectional study with an analytical component was conducted at the Faculty of Medicine, Mansoura University from December 2023 to May 2024. A sample of 598 students was selected using a convenience method. Our survey consisted of the Connor-Davidson Resilience Scale (CD-RISC-10), the Toronto Alexithymia Scale (TAS-20), and the NEO Five-Factor Inventory (NEO-FFI). IBM SPSS version 25 software was used for data analysis. Significance was set at p≤ 0.05.
Results: Almost half of the students showed low resilience (50.8%). Alexithymia was present in 25.3%. Significant associations were found between resilience and gender (p = 0.003), academic program (p < 0.05), and alexithymia (p = 0). Multivariate analysis revealed that positive predictors of high resilience were high levels of extraversion [ AOR= 4; CI= 1.29 – 12.46; p= 0.02] and conscientiousness (AOR= 8.203; CI= 2.58 – 26.13; p= 0.00), while high neuroticism was a negative predictor of high resilience (AOR= 0.5; 95℅CI= 0.01– 0.22; p= 0.00).
Conclusions: The majority of medical students exhibited low resilience, associated with higher levels of alexithymia and neuroticism and lower levels of extraversion and conscientiousness. Gender and the academic program also influenced resilience levels. Future research should examine the long-term relationship between resilience, alexithymia, and personality traits, and assess the effectiveness of resilience-building strategies. Strategies to enhance resilience and mental health programs are needed.
Characterisation of between-cluster heterogeneity in malaria cluster randomised trials to inform future sample size calculations
Authors: Biggs Joseph , Challenger Joseph , Dee Dominic , Elobolobo Eldo , Chaccour Carlos , Saute Francisco , Staedke Sarah , Vilakati Sibonakaliso , Benjamin-Chung Jade , Hsiang Michelle , Dabira Edgard , Erhart Annette , D’Alessandro Umberto , Tripura Rupam , Peto Thomas , von Seidlein Lorenz , Mukaka Mavuto , Mosha Jacklin , Protopopoff Natacha , Accrombessi Manfred , Hayes Richard , Churcher Thomas , Cook Jackie 
Published: February 2025

Abstract

Cluster randomised trials (CRTs) are important tools for evaluating the community-wide effect of malaria interventions. During the design stage, CRT sample sizes need to be inflated to account for the cluster-heterogeneity in measured outcomes. One such measure of heterogeneity, the coefficient of variation (k), is typically used in malaria CRTs yet is often estimated without prior data. Underestimation of k undermines study power and increases the probability of CRTs generating null results. We conducted a meta-analysis of cluster-summary data from 24 malaria CRTs and calculated true k values for prevalence and incidence outcomes using methods-of-moments and regression modelling approaches. Using random effects regression modelling we investigated the impact of empirical k values on original trial power, effect size uncertainty and explored associated factors. Results revealed empirical estimates of k often exceeded those used in sample size calculations which heavily contributed to compromised study power and effect size precision. Increased between-cluster heterogeneity of outcomes was associated with outcome measures (i.e. incidence or prevalence), lower endemicity, seasonality of surveys and uneven intervention coverage across clusters. Study findings can be used to inform future malaria CRT sample size calculations and trial design to help ensure malaria interventions are effectively and feasibly evaluated.
CO2 emissions due to melt-carbonate interactions triggered the Miocene Climatic Optimum
Authors: Zhang Guibin , Liu Shuaiqi , Zhang Lifei , Webb Alex , Bauer Kohen , McKenzie Neil , Guo Hongcheng 
Published: February 2025

Abstract

The Miocene Climatic Optimum (ca. 17−14 Ma) is an enigmatic climatic reversal that interrupted the long-term cooling trending of the Cenozoic. The forcing for this warming event remains unclear. Here we present direct evidence of melt−carbonate interactions in the Himalaya that produced globally significant CO2 emissions, and quantify resulting contributions to the atmosphere. The interactions occurred at high temperatures (685−828°C) in the lower crust (1.2−1.4 GPa) during the middle Miocene. Decoupled Sr−O isotope ratios suggest that calc-silicate zones were produced by infiltration of eclogite-derived granitic melts into carbonates during exhumation. Carbon budget estimates indicate that ~1270 kg of CO2 was liberated during the formation of each cubic meter of calc-silicates, and <4% of the carbon remained at the reaction site. Area extents of granite plutons vary from <10−1705 km2 in the Himalaya and each pluton contributed to carbon outfluxes of up to 1361× 103 kg/yr. As such, deep Earth degassing due to melt−carbonate interactions in the Himalayan orogen released 0.14−0.18 Pg/yr CO2 during the middle Miocene. With such degassing rates sustained over 0.1 Myr, corresponding atmospheric CO2 levels reached 400–600 ppmv within 0.1–0.3 Myr, ultimately enough to trigger the Miocene climate warming.
Investigation and analysis of life-related factors affecting the occurrence of colorectal adenomas and initial stages of colorectal cancer
Authors: Wang Ying , Zhong Hua , Li Yingying , Yang Dong 
Published: February 2025

Abstract

OBJECTIVE: To investigate the effects of different lifestyle habits on the occurrence of different types of colorectal adenomas and their initial stages of cancer.
Methods: 276 patients with colorectal tubular adenomas, 67 patients with colorectal serrated adenomas, and 70 patients with colorectal tubular adenomas with high-grade intraepithelial neoplasia, and 194 patients(control group ) underwent colonoscopy and were confirmed by pathology in the First Affiliated Hospital of Dalian Medical University during June 2023-December 2023. Lifestyle habits’ information was collected by questionnaire.
RESULTS: Males had a higher risk of developing colorectal tubular adenomas than females (OR 2.705, 95% CI 1.654, 4.426), P<0.001, whereas colorectal tubular adenomas evolving into high-grade intraepithelial neoplasia had no statistically difference (P>0.05). Smoking increased the risk of colorectal serrated adenoma development (OR 2.38, 95% CI 1.136, 4.984), P=0.022. Alcohol consumption raised the risk of colorectal tubular adenoma development (OR 1.447, 95% CI 1.033, 2.026) and it evolving to high-grade intraepithelial neoplasia (OR 1.75, 95% CI 1.257, 2.436). Chronic sleep duration of less than 6 hours per night increased the risk of developing colorectal tubular adenomas (OR 2.501, 95% CI 1.333, 4.692), P=0.004. Hypertension increased the risk of colorectal tubular adenomas evolving into high-grade intraepithelial neoplasia(OR 1.879, 95% CI 1.033, 3.417), P=0.039.
CONCLUSION: Males have a higher risk of developing colorectal tubular adenomas than females. Alcohol consumption and chronic sleep deprivation increase the risk of colorectal tubular adenoma development. Alcohol consumption and hypertension escalates the risk of initial-stage carcinoma of tubular adenoma. Smoking escalates the risk of developing serrated adenomas.