bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formulation of Nano-fertilizer and Phytochemical Screening of orange peel were utilized to enhance the growth of Vigna radiata
Authors: Iqbal Saba , Parveen Gulnaz , Ayub Ambreen , Gul Salma , Batool Tahira , Tara Nain , Sami Amtul , Khowaja Atiya Hussain 
Published: November 2023

Nanotechnology has developed into one of the most valued fields of sciences in the current century owing to the introduction of revolutionary changes in several disciplines of sciences. A key product of nanotechnology, i.e., nanoparticles (NPs) are abundantly used in modern research due to their novel applications. Orange peel contains vital nutrients that can be recycled into valuable material used for different purposes. In this study, we synthesized nano fertilizer from orange peel and tested its potential for promoting the growth of Vigna radiata and reducing the population of root rot pathogens. Nano-fertilizer extract was subjected to physical and chemical analyses for characterization. Transmission electron microscopy indicated spherical nanoparticles with sizes ranging between 18.22nm and 61.05nm. While the X-Ray diffraction analysis the peaks were observed at 2ɵ value of 19.830, 20.550, 21.240, 24.440, 27.330 and 29.770 which represented the similarity with urea slandered peak. The Synthesized nano-fertilizers contained phenols, steroids, triterpenes and xanthoproteins. In a screen house experiment, the nano fertilizer extract was administered to the seeds of Vigna radiata. The research demonstrated that increasing the dose of orange peel extract enhanced germination percentage by positively influencing plant growth characteristics. Meanwhile, a high concentration of orange peel amendment successfully reduced the colonization and infection percentage of root rotting pathogens when compared to the positive and negative controls.
Determinants of device success after transcatheter aortic valve replacement in patients with type-0 bicuspid aortic stenosis
Authors: Xiao Zhicheng , Yao Jing , Liu Xinmin , Yuan Fei , Yan Yunfeng , Luo Taiyang , Wang Moyang , Zhang Hongliang , Ren Faxin , Song Guangyuan 
Published: November 2023

BackgroundClinical evidence of transcatheter aortic valve replacement in patients with type-0 bicuspid aortic valve was relatively scarce.AimsOur goal was to explore determinants of device success after transcatheter aortic valve replacement in patients with type-0 bicuspid aortic valve morphology.MethodsIn this retrospective multicenter analysis, we included 59 patients with symptomatic severe aortic stenosis with type-0 bicuspid aortic valve morphology who underwent transcatheter aortic valve replacement. Type-0 bicuspid aortic valve was identified with multidetector computed tomography scans. The technical success rate was 89.8%, and the device success rate was 81.4%. Patients were divided into a device success group and a device failure group according to Valve Academic Research Consortium- 3 criteria.ResultsWhen we compared the two groups, we found that the ellipticity index of the aortic root and the presence of bulky calcifications at the commissure were statistically different (ellipticity index 35.7 ± 1.7 vs. 29.7 ± 1.1, p = 0.018; bulky calcification at the commissure, 54.5% vs. 4.5%, p &lt; 0.001). Further multivariate logistic analysis showed that bulky calcification at the commissure had a negative correlation with device success (odds ratio 0.030, 95% confidence interval 0.003–0.285, p = 0.002). Yet there was no statistical correlation between the ellipticity index and device success (odds ratio 0.818, 95% confidence interval 0.667–1.003, p = 0.053).ConclusionsThe presence of bulky calcifications at the commissure is negatively correlated with device success after transcatheter aortic valve replacement in patients with type-0 bicuspid aortic valve.
Association between GLO1 variants and gestational diabetes mellitus susceptibility in a Chinese population: a preliminary study
Authors: Zeng Qiaoli , Yang Taili , Wei Wenfeng , Zou Dehua , Wei Yue , Han Fengqiong , He Jieyun , Huang Jinzhi , Guo Runmin 
Published: November 2023

BackgroundGlyoxalase 1 (GLO1) plays a crucial role in defending against glycation. Single nucleotide polymorphism (SNP) variants in the GLO1 gene may affect gene expression and alter enzyme activity. However, there have been limited studies evaluating the association between GLO1 and diabetes, especially gestational diabetes mellitus (GDM). Therefore, this study is the first to explore the association of GLO1 SNPs and GDM risk.MethodsThe study included a total of 500 GDM patients and 502 control subjects. The SNPscan™ genotyping assay was used to genotype rs1781735, rs4746 and rs1130534. To assess the disparities in genotype, allele, and haplotype distributions and their correlation with GDM risk, the independent sample t-test, logistic regression, and chi-square test were employed during the data processing phase. Furthermore, one-way ANOVA was conducted to determine the differences in genotype and blood glucose and methylglyoxal(MG) levels.ResultsSignificant differences were observed in prepregnancy body mass index (pre-BMI), age, systolic blood pressure (SBP), diastolic blood pressure (DBP), and parity between GDM and healthy subjects (P &lt; 0.05). After adjusting for these factors, GLO1 rs1130534 TA remained associated with an increased risk of GDM (TA vs. TT + AA: OR = 1.320; 95% CI: 1.008-1.728; P = 0.044), especially in the pre-BMI ≥ 24 subgroup (TA vs. TT + AA: OR = 2.424; 95% CI: 1.048-5.607; P = 0.039), with fasting glucose levels being significantly elevated in the TA genotype compared to the TT genotype (P &lt; 0.05). Conversely, the GLO1 rs4746 TG was associated with a decreased risk of GDM (TG vs. TT: OR = 0.740; 95% CI: 0.548-0.999; P = 0.049; TG vs. TT + GG: OR = 0.740; 95% CI: 0.548-0.998; P = 0.048). Additionally, the haplotype T-G-T of rs1781735, rs4746 and rs1130534 was associated with a decreased risk of GDM among individuals with a pre-BMI ≥ 24 (OR = 0.423; 95% CI: 0.188-0.955; P = 0.038). Furthermore, the rs1781735 GG genotype was found to be more closely related to maternal MG accumulation and neonatal weight gain (P &lt; 0.05).ConclusionOur findings suggested that GLO1 rs1130534 was associated with an increased susceptibility to GDM and higher blood glucose levels, but GLO1 rs4746 was associated with a decreased risk of GDM. The rs1781735 has been associated with the accumulation of maternal MG and subsequent weight gain in neonates.
Childhood adversity predicts stronger premenstrual mood worsening, stress appraisal and cortisol decrease in women with Premenstrual Dysphoric Disorder
Authors: Nayman Sibel , Schricker Isabelle Florence , Reinhard Iris , Kuehner Christine 
Published: November 2023

BackgroundLifetime traumatic events are prevalent in women with Premenstrual Dysphoric Disorder (PMDD) and predict stronger premenstrual symptom intensities. Less is known about the unique effects of childhood adversity on PMDD. This study aims to investigate the menstrual cycle related course of mood, stress appraisal and cortisol activity over time and the effects of childhood adversity – by controlling for recent stressful life events – on the cyclicity of these outcomes.MethodsFifty-two women with PMDD completed questionnaires on childhood adversity and stressful life events during the past 12 months. Momentary negative and positive affect, stress appraisal, and saliva-cortisol were assessed within an Ambulatory Assessment (AA) design over four consecutive days during both the follicular and the late luteal phase. This AA was repeated after five months, resulting in two measurement bursts.ResultsWomen with PMDD showed expected cycle related variations in mood and stress appraisal, whereby these effects weakened over time. No cortisol cyclicity was identified. Higher childhood adversity was linked to stronger increases in negative affect and stress appraisal, and stronger decreases in positive affect from the follicular toward the late luteal phase. Women with higher childhood adversity exhibited lower cortisol levels during the late luteal phase compared to the follicular phase whereas no such cyclicity was found in women with lower childhood adversity.ConclusionChildhood adversity appears to show independent deteriorating effects on premenstrual mood worsening and stress appraisal in women with PMDD. The observed cortisol cyclicity in women with higher childhood adversity may point to different neuroendocrine subtypes of PMDD in relation to childhood trauma and requires further systematic research.
Association between systemic lupus erythematosus and inflammatory bowel disease in European and East Asian populations: a two-sample Mendelian randomization study
Authors: Xie Weidong , Jiang Haojie , Chen Yao , Zhang Huanhao , Song Yaoyu , Yu Zhaojie , Gu Huayan , Xu Hongkai , Han Saiyi , Li Sen , Liu Naxin , Han Shaoliang 
Published: November 2023

BackgroundPrevious studies have shown a coexistence phenomenon between systemic lupus erythematosus (SLE) and inflammatory bowel disease (IBD), but the causal relationship between them is still unclear. Therefore, we conducted a two-sample Mendelian randomization (MR) analysis using publicly available summary statistics data to evaluate whether there was a causal relationship between the two diseases.MethodsSummary statistics for SLE and IBD were downloaded from the Open Genome-Wide Association Study and the International Inflammatory Bowel Disease Genetics Consortium. European and East Asian populations were included in this MR work. We adopted a series of methods to select instrumental variables that are closely related to SLE and IBD. To make the conclusion more reliable, we applied a variety of different analysis methods, among which the inverse variance–weighted (IVW) method was the main method. In addition, heterogeneity, pleiotropy, and sensitivity were assessed to make the conclusions more convincing.ResultsIn the European population, a negative causal relationship was observed between SLE and overall IBD (OR = 0.94; 95% CI = 0.90, 0.98; P &lt; 0.004) and ulcerative colitis (UC) (OR = 0.93; 95% CI = 0.88, 0.98; P = 0.006). After removing outliers with Mendelian Randomization Pleiotropy RESidual Sum and Outlier (MR-PRESSO), the results remained consistent with IVW. However, there was no causal relationship between SLE and Crohn’s disease. In the East Asian population, no causal relationship was found between SLE and IBD.ConclusionOur results found that genetic susceptibility to SLE was associated with lower overall IBD risk and UC risk in European populations. In contrast, no association between SLE and IBD was found in East Asian populations. This work might enrich the previous research results, and it may provide some references for research in the future.
Characterizing the weather band variability of the Texas shelf current
Authors: Nwankwo Uchenna C. , DiMarco Steven F. , Nowak Heather M. , Whilden Kerri , Buckingham Brian , Walpert John , Knap Anthony H. 
Published: November 2023

Considering the benefits of understanding the circulation patterns of the shelf, it is not surprising that there are numerous studies of the Texas Shelf circulation patterns. Given that previous studies were focused on the low-frequency variability of the circulation which is upcoast (northeast flow) in the summer and downcoast (southwest flow) especially on the inner shelf in the non-summer seasons, this study investigates the weather band (2–15 days) variability of the Texas Shelf near-surface circulation pattern. Current meter data at 1.5 m below the sea surface from the inner, mid, and outer shelves were analyzed. This study demonstrated that there are high-frequency current reversals within the weather band in each season. From the estimated persistence of the currents during reversals, the inner and mid shelf currents are predominantly downcoast in the non-summer seasons and upcoast in the summer season whereas the outer shelf currents are mostly upcoast all year round. The Wavelets analysis of the currents revealed that most of the variabilities on the inner and mid shelf regions were within the 4-12-day band whereas on the outer shelf the dominant variability was within the 3–8-day band. From the cross-spectra analysis of both the currents and wind data, it was determined that the influence of the wind was more dominant on the inner and mid shelf regions at the 8–15-day band than on the outer shelf where the contribution of the wind is prevalent at the 2–4-day band.
Effect of matrix suction on the shear strength characteristics of reinforced granite residual soil
Authors: Deng Xiangwen , Zhu Zhengyu , Lin Xi yang , Yang Xue qiang 
Published: November 2023

Introduction: The soil in geogrid-reinforced structures is typically unsaturated, with the shear strength provided by both the matrix suction and the reinforced body. Traditional structural designs for saturated soils only consider the shear strength provided by the reinforced body, neglecting the part provided by matrix suction. As a result, the design for reinforced structures is biased toward conservatism.Method: The study examined the matrix suction-provided shear strength in reinforced soils through strain-controlled triaxial and soil-water characteristic curve (SWCC) pressure plate instrumentation. The feasibility of the Schrefler and Khalili unsaturated soil shear strength formulas for predicting shear strength based on matrix suction forces was verified.Results: The study revealed that the cohesion of saturated reinforced soil exhibits a significant decrease in contrast with unsaturated reinforced soil, with matrix suction serving as a crucial consideration for reinforced structure design.Discussion: The experimental results confirm the suitability of applying the quasi-cohesion increment theory to reinforced clays. The Khalili formula can be utilized to predict the quasi cohesion of unsaturated reinforced soils with greater accuracy under diverse dry density conditions. The results obtained using post-shear moisture content were closer to the measured values than those using initial moisture content.
Effect of pH and medium composition on chain elongation with Megasphaera hexanoica producing C4-C8 fatty acids
Authors: Fernández-Blanco Carla , Veiga María C. , Kennes Christian 
Published: November 2023

IntroductionChain elongation technology, which involves fermentation with anaerobic bacteria, has gained attention for converting short and medium chain substrates into valuable and longer-chain products like medium chain fatty acids (MCFAs). In the recent past, the focus of studies with pure chain elongating cultures was on species of other genera, mainly Clostridium kluyveri. Recently, other chain elongators have been isolated that deserve further research, such as Megasphaera hexanoica.MethodsIn this study, batch studies were performed in bottles with two different media to establish the optimal conditions for growth of M. hexanoica: (a) a medium rich in different sources of nitrogen and (b) a medium whose only source of nitrogen is yeast extract. Also, batch bioreactor studies at pH values of 5.8, 6.5 and 7.2 were set up to study the fermentation of lactate (i.e., electron donor) and acetate (i.e., electron acceptor) by M. hexanoica.Results and discussionBatch bottle studies revealed the yeast extract (YE) containing medium as the most promising in terms of production/cost ratio, producing n-caproate rapidly up to 2.62 ± 0.24 g/L. Subsequent bioreactor experiments at pH 5.8, 6.5, and 7.2 confirmed consistent production profiles, yielding C4-C8 fatty acids. A fourth bioreactor experiment at pH 6.5 and doubling both lactate and acetate concentrations enhanced MCFA production, resulting in 3.7 g/L n-caproate and 1.5 g/L n-caprylate. H2 and CO2 production was observed in all fermentations, being especially high under the increased substrate conditions. Overall, this study provides insights into M. hexanoica’s behavior in lactate-based chain elongation and highlights optimization potential for improved productivity.
A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
Authors: Di Stefano Mariantonietta , Ismail Mona H. , Leitner Thomas , Faleo Giuseppina , Alwazzeh Marwan Jabr , Mbisa Jean Lutamyo , Fiore Josè Ramon , Santantonio Teresa Antonia 
Published: November 2023

Background and aimHepatitis C virus (HCV) infection is a major global public health concern, being a leading cause of chronic liver diseases such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The virus is classified into 8 genotypes and 93 subtypes, each displaying distinct geographic distributions. Genotype 4 is the most predominant in the Middle East and Eastern Mediterranean and is associated with high rates of hepatitis C infection worldwide. This study used next-generation sequencing to fully characterize the HCV genome and identify a novel subtype within genotype 4 isolated from a 64-year-old Saudi man diagnosed with hepatitis C.MethodsWe analyzed the complete genome of the 141-HCV isolate using whole-genome sequencing.ResultsOur phylogenetic reconstructions, based on the entire genome of HCV-4 strains, revealed that the 141-HCV isolate formed a distinct group within the genotype 4 classification, providing valuable new insights into the variability of HCV.ConclusionThis discovery of a previously unclassified HCV subtype within genotype 4 sheds light on the ongoing evolution and diversity of the virus. Such knowledge has significant implications for diagnostic and therapeutic approaches, as different subtypes may exhibit varying drug sensitivities and resistance profiles.
The gut microbiome dysbiosis and regulation by fecal microbiota transplantation: umbrella review
Authors: Zhang Xianzhuo , Luo Xufei , Tian Liang , Yue Ping , Li Mengyao , Liu Kefeng , Zhu Daoming , Huang Chongfei , Shi Qianling , Yang Liping , Xia Zhili , Zhao Jinyu , Ma Zelong , Li Jianlong , Leung Joseph W. , Lin Yanyan , Yuan Jinqiu , Meng Wenbo , Li Xun , Chen Yaolong 
Published: November 2023

BackgroundGut microbiome dysbiosis has been implicated in various gastrointestinal and extra-gastrointestinal diseases, but evidence on the efficacy and safety of fecal microbiota transplantation (FMT) for therapeutic indications remains unclear.MethodsThe gutMDisorder database was used to summarize the associations between gut microbiome dysbiosis and diseases. We performed an umbrella review of published meta-analyses to determine the evidence synthesis on the efficacy and safety of FMT in treating various diseases. Our study was registered in PROSPERO (CRD42022301226).ResultsGut microbiome dysbiosis was associated with 117 gastrointestinal and extra-gastrointestinal. Colorectal cancer was associated with 92 dysbiosis. Dysbiosis involving Firmicutes (phylum) was associated with 34 diseases. We identified 62 published meta-analyses of FMT. FMT was found to be effective for 13 diseases, with a 95.56% cure rate (95% CI: 93.88–97.05%) for recurrent Chloridoids difficile infection (rCDI). Evidence was high quality for rCDI and moderate to high quality for ulcerative colitis and Crohn’s disease but low to very low quality for other diseases.ConclusionGut microbiome dysbiosis may be implicated in numerous diseases. Substantial evidence suggests FMT improves clinical outcomes for certain indications, but evidence quality varies greatly depending on the specific indication, route of administration, frequency of instillation, fecal preparation, and donor type. This variability should inform clinical, policy, and implementation decisions regarding FMT.
A novel deep learning model for glioma epilepsy associated with the identification of human cytomegalovirus infection injuries based on head MR
Authors: Wang Wei , Li Xuanyi , Ye Lou , Yin Jian 
Published: November 2023

PurposeIn this study, a deep learning model was established based on head MRI to predict a crucial evaluation parameter in the assessment of injuries resulting from human cytomegalovirus infection: the occurrence of glioma-related epilepsy. The relationship between glioma and epilepsy was investigated, which serves as a significant indicator of labor force impairment.MethodsThis study enrolled 142 glioma patients, including 127 from Shengjing Hospital of China Medical University, and 15 from the Second Affiliated Hospital of Dalian Medical University. T1 and T2 sequence images of patients’ head MRIs were utilized to predict the occurrence of glioma-associated epilepsy. To validate the model’s performance, the results of machine learning and deep learning models were compared. The machine learning model employed manually annotated texture features from tumor regions for modeling. On the other hand, the deep learning model utilized fused data consisting of tumor-containing T1 and T2 sequence images for modeling.ResultsThe neural network based on MobileNet_v3 performed the best, achieving an accuracy of 86.96% on the validation set and 75.89% on the test set. The performance of this neural network model significantly surpassed all the machine learning models, both on the validation and test sets.ConclusionIn this study, we have developed a neural network utilizing head MRI, which can predict the likelihood of glioma-associated epilepsy in untreated glioma patients based on T1 and T2 sequence images. This advancement provides forensic support for the assessment of injuries related to human cytomegalovirus infection.
Use of AgomiR and AntagomiR technologies to alter satellite cell proliferation in vitro, miRNA expression, and muscle fiber hypertrophy in intrauterine growth-restricted lambs
Authors: Greene M. A. , Worley G. A. , Udoka A. N. S. , Powell R. R. , Bruce T. , Klotz J. L. , Bridges W. C. , Duckett S. K. 
Published: November 2023

Introduction: microRNAs (miRNAs) are small non-coding RNAs that work at the posttranscriptional level to repress gene expression. Several miRNAs are preferentially expressed in skeletal muscle and participate in myogenesis. This research was conducted to alter endogenous miRNA expression in skeletal muscle to promote muscle hypertrophy.Methods: Two experiments were conducted using mimic/agomiR or antagomir technologies to alter miRNA expression and examine changes in myoblast proliferation in vitro (experiment 1) and muscle hypertrophy in vivo (experiment 2). In vitro experiments found that antagomiR-22-3p and mimic-127 increased myoblast proliferation compared to other miRNA treatments or controls. These miRNA treatments, antagomiR-22-3p (ANT22) and agomiR-127 (AGO127), were then used for intramuscular injections in longissimus muscle.Results and discussion: The use of antagomiR or mimic/agomiR treatments down-regulated or up-regulated, respectively, miRNA expression for that miRNA of interest. Expression of predicted target KIF3B mRNA for miR-127 was up-regulated and ACVR2a mRNA was up-regulated for miR-22-3p. ANT22 injection also up-regulated the major regulator of protein synthesis (mTOR). Proteomic analyses identified 11 proteins for AGO127 and 9 proteins for ANT22 that were differentially expressed. Muscle fiber type and cross-sectional area were altered for ANT22 treatments to transition fibers to a more oxidative state. The use of agomiR and antagomir technologies allows us to alter miRNA expression in vitro and in vivo to enhance myoblast proliferation and alter muscle fiber hypertrophy in IUGR lambs during early postnatal growth.