bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of Early Postoperative Bleeding After OAGB: A Single-center Experience
Authors: Helmy Ramy F. , Elbanna Mohey R. , El-Zemeity Ahmed O. , Abozeid Tarek A. O. , Fadl Ehab M. 
Published: October 2023

Introduction:
One anastomosis gastric bypass (OAGB) has gained popularity among bariatric surgeons due to the shorter operative time, fewer sites for anastomotic leaks and internal herniation, shorter learning curve, ease of reversibility and revision with equivalent results to Roux en Y gastric bypass in terms of weight loss and co-morbidity resolution. We present our experience in managing early postoperative bleeding after OAGB.

Patients and Methods:
Patients who underwent OAGB in Bariatric Surgery Unit, Ain Shams University Hospitals between January 2016 and January 2023 were followed up for 30 days for early postoperative complications. Patients were 210 females (70%) and 90 males, with a mean age of 41.3±7.1 years, and mean preoperative body mass index of 45.2±6.1 kg/m². The incidence of early postoperative bleeding, the sites of the bleeding and management strategy were reported.

Results:
Fourteen of 300 patients (4.67%) developed early postoperative bleeding after OAGB. Intraluminal bleeding occurred in 4 patients, 3 of which were controlled by endoscopy and one by laparoscopic suturing. Intra-abdominal bleeding occurred in 10 patients, 7 of which were successfully managed conservatively, and 3 required laparoscopic management. Two cases had both intra-abdominal bleeding and intraluminal bleeding in the distal stomach, confirmed by intraoperative endoscopy, and controlled by laparoscopic suturing.

Conclusion:
Early postoperative bleeding after OAGB is a rare complication (4.67%). Conservative treatment is more successful in controlling intra-abdominal bleeding. In case of failed conservative treatment, laparoscopy is the method of choice. Most cases of intra-luminal bleeding need early endoscopic intervention.
Transabdominal Management of Epiphrenic Diverticula in the Setting of Achalasia: A Single-center Review
Authors: Tankel James , Meng Andrew , Gold Morgan , Mueller Carmen , Spicer Jonathan , Cools-Lartigue Jonathan , Ferri Lorenzo , Najmeh Sara 
Published: October 2023

Background:
The perioperative and functional outcomes of patients with epiphrenic diverticula (ED) on a background of achalasia managed via a minimally invasive transabdominal approach are under-reported. We describe our center’s experience over 10 years of treating such patients.

Methods:
A single-center, retrospective chart of a prospectively maintained hospital database was performed. All patients with a diagnosis of ED and manometrically proven achalasia were identified. Demographic, clinical, and surgical data were extracted from the institution’s medical records. Patients were stratified by whether they underwent myotomy only or myotomy plus diverticulectomy and compared in a univariate manner.

Results:
There were 18 patients who met the inclusion criteria. The median age of the cohort was 67.1 years (range 53.1 to 77.8), the maximal size of the diverticula was 3.5 cm (range 2.0 to 7.0), and the distance of the proximal lip of the diverticulum to the incisors was 33.5 cm (range 28.0 to 38.0). In terms of surgical intervention, 14 patients (77.8%) underwent myotomy plus diverticulectomy, and 4 (22.2%) underwent myotomy alone. The duration of surgery was significantly longer in the former (177.5 vs. 75.0 min, P=0.031). In total, 9/18 (50.0%) of patients were discharged on the day of surgery. There was a trend to more major postoperative complications following diverticulectomy plus myotomy, with 2/13 (15.4%) patients suffering staple line leaks. Excellent long-term functional outcomes were achieved, with 81.3% of patients having sustained resolution of their symptoms.

Conclusions:
Laparoscopic transabdominal approach for the treatment of ED offers an acceptable risk profile and favorable functional outcomes in patients with underlying achalasia.
Effectiveness of exercise interventions on urinary incontinence and pelvic organ prolapse in pregnant and postpartum women: umbrella review and clinical guideline development
Authors: Ryhtä Iina , Axelin Anna , Parisod Heidi , Holopainen Arja , Hamari Lotta 
Published: October 2023

ABSTRACT

Introduction and aims:
Dysfunction related to pelvic floor muscles (PFM) is common among pregnant and postpartum women and can cause symptoms such as urinary incontinence or pelvic organ prolapse (POP). As part of developing a nationwide clinical practice guideline for nursing in Finland, the aim of this umbrella review is to summarize the existing evidence about the effectiveness of exercise interventions on urinary incontinence and POP in pregnant and postpartum women. To promote knowledge translation, recommendations for health care professionals are presented.

Methods:
We conducted an umbrella review to summarize the existing evidence. The JBI methodology for umbrella reviews was used to guide the review. The level of evidence was evaluated using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach, and recommendations based on the evidence were formulated by a clinical guideline working group.

Results:
Altogether, 9 systematic reviews, reporting findings from 89 original studies, were included. The methodological quality of the reviews was evaluated using JBI's checklist. The highest level of evidence was found for preventing the symptoms of postpartum urinary incontinence through exercise and pelvic floor muscle training (PFMT) during pregnancy. Moderate-level evidence showed that exercise and PFMT are likely to reduce the symptoms and severity of urinary incontinence, but the level of evidence was low on PFMT reducing the symptoms of POP.

Conclusion:
We recommend encouraging and guiding pregnant and postpartum women to exercise and train PFM. We also recommend identifying pregnant and postpartum women with symptoms of PFM dysfunction and directing them to a physiotherapist or other health care professional specializing in pelvic floor function.
Mutant mice lacking alternatively spliced p53 isoforms unveilAckr4as a male-specific prognostic factor in Myc-driven B-cell lymphomas
Authors: Fajac Anne , Simeonova Iva , Leemput Julia , Gabriel Marc , Morin Aurélie , Lejour Vincent , Hamon Annaïg , Rakotopare Jeanne , Vaysse-Zinkhöfer Wilhelm , Eldawra Eliana , Pinskaya Marina , Morillon Antonin , Bourdon Jean-Christophe , Bardot Boris , Toledo Franck 
Published: October 2023

AbstractTheTrp53gene encodes several isoforms of elusive biological significance. Here we show that mice lacking theTrp53Alternatively Spliced (AS) exon, thereby expressing the canonical p53 protein but not isoforms with the AS C-terminus, have unexpectedly lost a male-specific protection against Myc-induced B-cell lymphomas. Lymphomagenesis was delayed in p53+/+Eμ-Myc males compared to p53ΔAS/ΔASEμ-Myc males, but also compared to p53+/+Eμ-Myc and p53ΔAS/ΔASEμ-Myc females. Pre-tumoral splenic cells from p53+/+Eμ-Myc males exhibited a higher expression ofAckr4,encoding an atypical chemokine receptor with tumor suppressive effects. We identifiedAckr4as a p53 target gene whose p53-mediated transactivation is inhibited by estrogens, and as a male-specific factor of good prognosis relevant for murine Eμ-Myc-induced and human Burkitt lymphomas. Furthermore, the knockout ofACKR4increased the chemokine-guided migration of Burkitt lymphoma cells. These data demonstrate the functional relevance of alternatively spliced p53 isoforms and reveal sex disparities in Myc-driven lymphomagenesis.
SODA: Software to Support the Curation and Sharing of FAIR Autonomic Nervous System Data
Authors: Marroquin Christopher , Clark Jacob , Portillo Dorian , Soundarajan Sanjay , Patel Bhavesh 
Published: October 2023

AbstractSince 2014, the National Institutes of Health (NIH)’s Stimulating Peripheral Activity to Relieve Conditions (SPARC) Program has been supporting research and development of therapeutic devices that modulate electrical activity in the autonomic nervous system (ANS) to improve organ function, also known as bioelectronic medicine. To optimize the reusability of data resulting from ANS-related research, the SPARC Program also supported the development of guidelines for curating and sharing data in line with the FAIR (Findable, Accessible, Interoperable, Reusable) Principles. These guidelines are exhaustive to maximize FAIRness of data but as a result, they are difficult and time-consuming for researchers to implement. To address these challenges, we developed SODA (Software to Organize Data Automatically), an open source and free cross-platform desktop software that guides researchers step-by-step in preparing and sharing their ANS-related data according to the SPARC guidelines. SODA combines intuitive user interfaces with automation to streamline the process and reduce researchers’ time, effort, and error in making their data FAIR. We provide in this paper an overview of SODA and results of testing its performance. We also provide an overview of the impact of SODA which is, to our knowledge, the first researcher-oriented tool for making data FAIR.
Systems-level analyses dissociate genetic regulators of reactive oxygen species and energy production
Authors: Bennett Neal K. , Lee Megan , Orr Adam L. , Nakamura Ken 
Published: October 2023

AbstractRespiratory chain dysfunction can decrease ATP and increase reactive oxygen species (ROS) levels. Despite the importance of these metabolic parameters to a wide range of cellular functions and disease, we lack an integrated understanding of how they are differentially regulated. To address this question, we adapted a CRISPRi- and FACS-based platform to compare the effects of respiratory gene knockdown on ROS to their effects on ATP. Focusing on genes whose knockdown is known to decrease mitochondria-derived ATP, we showed that knockdown of genes in specific respiratory chain complexes (I, III and CoQ10 biosynthesis) increased ROS, whereas knockdown of other low ATP hits either had no impact (mitochondrial ribosomal proteins) or actually decreased ROS (complex IV). Moreover, although shifting metabolic conditions profoundly altered mitochondria-derived ATP levels, it had little impact on mitochondrial or cytosolic ROS. In addition, knockdown of a subset of complex I subunits—including NDUFA8, NDUFB4, and NDUFS8—decreased complex I activity, mitochondria-derived ATP and supercomplex level, but knockdown of these genes had differential effects on ROS. Conversely, we found an essential role for ether lipids in the dynamic regulation of mitochondrial ROS levels independent of ATP. Thus, our results identify specific metabolic regulators of cellular ATP and ROS balance that may help dissect the roles of these processes in disease and identify therapeutic strategies to independently target energy failure and oxidative stress.SignificanceMitochondrial respiration generates both energy (ATP) and reactive oxygen species (ROS). Insufficient energy and increased ROS from respiratory chain dysfunction may be central to the pathophysiology of neurodegenerative diseases and aging. We established a screening platform using CRISPR and fluorescent-cell sorting to compare the impact of decreasing respiratory chain proteins on ROS and ATP levels. The results provide the first systems-level analysis of how ROS and ATP are differentially regulated, and identify genes and respiratory chain complexes that can manipulate each independently. These findings advance our understanding of the relative contributions of ATP and ROS to disease pathophysiology, and guide the development of therapies to preserve energy while minimizing ROS.
Tissue-resident NK cells support survival in pancreatic cancer through promotion of cDC1-CD8T activity
Authors: Go Simei , Demetriou Constantinos , Valenzano Giampiero , Hughes Sophie , Lanfredini Simone , Ferry Helen , Arbe-Barnes Edward , Sivakumar Shivan , Bashford-Rogers Rachael , Middleton Mark R. , Mukherjee Somnath , Morton Jennifer , Jones Keaton , O’Neill Eric 
Published: October 2023

AbstractThe immunosuppressive microenvironment in pancreatic ductal adenocarcinoma (PDAC) prevents tumor control and strategies to restore anti-cancer immunity (i.e. by increasing CD8 T cell activity) have had limited success. Here we demonstrate how inducing localized physical damage using ionizing radiation (IR) unmasks the benefit of immunotherapy by increasing tissue-resident NK (trNK) cells that support CD8 T activity. Our data confirms that targeting mouse orthotopic PDAC tumors with IR together with CCR5 inhibition and PD1 blockade reduces E-cadherin positive tumor cells by recruiting a hypoactive NKG2D-veNK population, phenotypically reminiscent of trNK cells, that supports CD8 T cell involvement. We show an equivalent population in human PDAC cohorts that represents immunomodulatory trNK cells that could similarly support CD8 T cell levels in a cDC1-dependent manner. Importantly, a trNK signature associates with survival in PDAC and solid malignancies revealing a potential beneficial role for trNK in improving adaptive anti-tumor responses and supporting CCR5i/αPD1 and IR-induced damage as a novel therapeutic approach.
hkbis required forDIP-αexpression and target recognition in theDrosophilaneuromuscular circuit
Authors: Wang Yupu , Salazar Rio , Simonetta Luciano , Sorrentino Violet , Gatton Terrence J. , Wu Bill , Vecsey Christopher G. , Carrillo Robert A. 
Published: October 2023

AbstractOur nervous system contains billions of neurons that form precise connections with each other through interactions between cell surface proteins (CSPs). InDrosophila, the Dpr and DIP immunoglobulin protein subfamilies form homophilic or heterophilic interactions to instruct synaptic connectivity, synaptic growth and cell survival. However, the upstream regulation and downstream signaling mechanisms of Dprs and DIPs are not clear. In theDrosophilalarval neuromuscular system,DIP-αis expressed in the dorsal and ventral type-Is motor neurons (MNs). We conducted an F1 dominant modifier genetic screen to identify regulators of Dprs and DIPs. We found that the transcription factor,huckebein(hkb), genetically interacts withDIP-αand is important for target recognition specifically in the dorsal Is MN, but not the ventral Is MN. Loss ofhkbled to complete removal ofDIP-αexpression. We then confirmed that this specificity is through the dorsal Is MN specific transcription factor,even-skipped(eve), which acts downstream ofhkb. Genetic interaction betweenhkbandeverevealed that they act in the same pathway to regulate dorsal Is MN connectivity. Our study provides insight into the transcriptional regulation ofDIP-αand suggests that distinct regulatory mechanisms exist for the same CSP in different neurons.
Detecting copy number variations from single-cell chromatin sequencing data by AtaCNV
Authors: Wang Xiaochen , Jin Zijie , Shi Yang , Xi Ruibin 
Published: October 2023

AbstractSinge-cell assay of transposase accessible chromatin sequencing (scATAC-seq) can unbiasedly profile genome-wide chromatin accessibility in single cells. In single-cell tumor studies, identification of normal cells or tumor clonal structures often rely on copy number variations (CNVs). However, CNV detection from scATAC-seq is difficult due to the high noise, sparsity, and confounding factors. Here, we describe AtaCNV, a computational algorithm that accurately detects high resolution CNVs from scATAC-seq data. We benchmark AtaCNV using simulation and real data and find AtaCNV’s superior performance. Analyses of 10 scATAC-seq datasets shows that AtaCNV could effectively distinguish malignant from non-malignant cells. In glioblastoma, endometrial and ovarian cancer samples, AtaCNV identifies subclones at distinct cellular states, suggesting important interplay between genetic and epigenetic plasticity. Some tumor subclones only differ in small-scale CNVs, demonstrating the importance of high-resolution CNV detection. These data show that AtaCNV can aid the integrative analysis for understanding the complex heterogeneity in cancer.
Longitudinal prevalence of neurogenic orthostatic hypotension in the idiopathic Parkinson Progression Marker Initiative (PPMI) cohort
Authors: Beach Paul , McKay J. Lucas 
Published: October 2023

AbstractBackgroundReported orthostatic hypotension (OH) prevalence in Parkinson disease (PD) varies widely, with few studies evaluating specifically neurogenic-OH (nOH). The ratio of orthostatic heart rate (HR) to systolic blood pressure (SBP) change (Δ) is a valid screening method to stratify nOH/non-nOH but had minimal epidemiologic application.ObjectiveTo estimate the prevalence of nOH and non-nOH in the PPMI using the ΔHR/ΔSBP ratio and examine associations between nOH and various motor and non-motor measures.MethodsLongitudinal orthostatic vitals and motor and non-motor measures were extracted (baseline-month 48). Patients were consensus criteria classified as OH+/-, with ΔHR/ΔSBP sub-classification to nOH (ΔHR/ΔSBP<0.5) or non-nOH (ratio≥0.5). Prevalence was determined across visits. Independent linear mixed models tested associations between nOH/non-nOH and clinical variables.ResultsOf N=907 PD with baseline orthostatic vitals, 3.9% and 1.8% exhibited nOH and non-nOH, respectively. Prevalence of nOH/non-nOH increased yearly (P=0.012, chi-square), though with modest magnitude (baseline: 5.6% [95% CI: 4.3-7.3%]; month 48: 8.6% [6.4-11.5%]). nOH patients were older than PD with no OH and nOH was associated with greater impairment of motor and independent functioning than non-nOH/OH-groups. Cognitive function and typical OH symptoms were worse in PD+OH, generally.ConclusionsnOH prevalence was greater than non-nOH in the PPMI early PD cohort, with modest prevalence increase over time. Our findings are consistent with prior studies of larges cohorts that evaluated nOH, specifically. Early PD with nOH were likelier to be older and suffer from greater motor and functional impairment, but OH presence was generally associated with more cognitive impairment.
Cognitive Impairment and Noncommunicable Diseases in Egypt’s Aging Population: Insights and Implications from the pilot of the Longitudinal Study of Egyptian Healthy Aging “AL-SEHA”
Authors: Moustafa Sara A. , Deif Reem , Gaballah Nada , Salama Mohamed 
Published: October 2023

AbstractAs the global population ages, the prevalence of cognitive impairment among older individuals has been steadily rising. Egypt, like many countries, is grappling with the challenges posed by an aging demographic. Cognitive impairment not only affects the quality of life of older adults but also imposes significant burdens on healthcare systems and societies as a whole. This paper presents findings from the pilot phase of the Longitudinal Study of Egyptian Healthy Aging, known as “AL-SEHA,” shedding light on the intricate relationship between cognitive impairment and noncommunicable diseases (NCDs) in Egypt’s aging population.KEY MESSAGESThe increasing prevalence of cognitive impairment among the aging population creates a need for a comprehensive understanding of its implications on the individuals’ quality of lifeA significant association between cognitive impairment and non-communicable diseases exists and further research can help in the strategic allocation of resources for designing the most effective interventions
Cardiopulmonary resuscitation (CPR) competency retention among registered nurses in critical care versus general care unit
Authors: AL-Helih Yahia , AL-Ruzzieh Majeda , AL-Yatim Sami , Alawneh Mohammad , Haija Saleh Abu-AL , Odeh Faten 
Published: October 2023

AbstractBackgroundCardiac arrest is a sudden and life-threatening event resulting in an end of cardiac activity, necessitating immediate intervention to prevent fatalities. In-hospital cardiac arrest (IHCA) presents a critical medical emergency, demanding swift and competent response. Cardiopulmonary resuscitation (CPR) is a key lifesaving intervention for IHCA, but the frequency of CPR events and the decay of CPR skills and knowledge among healthcare professionals (HCPs) raise concerns.Methods and ResultsIn a prospective quasi-experimental study with no control group, 263 registered nurses (121 in critical care units and 144 in general care units) were assessed for CPR skills and knowledge retention at various time intervals. The result showed that overall decay after six months are almost the same for both groups. Knowledge decay started for both groups after one month and for both groups the highest level of decay was reported after three months, however the decay pattern was quite different. General units mean differences showed sudden sharp decline on three months which was not reported with critical care nurses who showed steady decay reaching to six months.ConclusionsIn this study, it is noteworthy that nurses in critical care units exhibited consistent decay in skills and knowledge, while those in general care units demonstrated a progressive decline over time.
Direct delivery of Cas9 or base editor protein and guide RNA complex enables genome editing in the retina
Authors: Botto Catherine , Pulman Juliette , Malki Hugo , Ren Duohao , Oudin Paul , Cian Anne De , As Marie , Izabelle Charlotte , Saubamea Bruno , Fouquet Stéphane , Robert Camille , El-Amraoui Aziz , Fisson Sylvain , Concordet Jean-Paul , Dalkara Deniz 
Published: October 2023

AbstractGenome editing by CRISPR-Cas holds promise for the treatment of retinal dystrophies. For therapeutic gene editing, transient delivery of CRISPR- Cas9 is preferable to viral delivery which leads to long-term expression with potential adverse consequences. Successful delivery of Cas9 protein and its guide RNA as ribonucleoprotein (RNP) complexes has been reported in the retinal pigment epitheliumin vivobut not into photoreceptors, the main target of retinal dystrophies. Here, we investigate the feasibility of direct RNP delivery to photoreceptors and RPE cells. We show that RNPs composed of Cas9 or adenine- base editor and guide RNA, without addition of any carrier compounds, induce gene editing in retinal cells at variable rates depending on the guide RNA efficiency and on the locus. But Cas9 RNP delivery at high concentrations leads to outer retinal toxicity indicating a need to improve delivery efficiency for future therapeutic use.