bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicted Role of Acetyl-CoA Synthetase and HAT p300 in Extracellular
Lactate Mediated Lactylation in the Tumor: In vitro and In silico Models
Authors: Patel Rushikesh , Raj Ajay K. , Lokhande Kiran B. , Joshi Mrudula , Khunteta Kratika , Pal Jayanta K. , Sharma Nilesh K. 
Published: December 2023

Background:
As per the Warburg effect, cancer cells are known to convert pyruvate into
lactate. The accumulation of lactate is associated with metabolic and epigenetic reprogramming,
which has newly been suggested to involve lactylation. However, the role of secreted lactate in
modulating the tumor microenvironment through lactylation remains unclear. Specifically, there are
gaps in our understanding of the enzyme responsible for converting lactate to lactyl-CoA and the
nature of the enzyme that performs lactylation by utilizing lactyl-CoA as a substrate. It is worth noting that there are limited papers focused on metabolite profiling that detect lactate and lactyl-CoA
levels intracellularly and extracellularly in the context of cancer cells.

Methods:
Here, we have employed an in-house developed vertical tube gel electrophoresis (VTGE)
and LC-HRMS assisted profiling of extracellular metabolites of breast cancer cells treated by anticancer compositions of cow urine DMSO fraction (CUDF) that was reported previously. Furthermore, we used molecular docking and molecular dynamics (MD) simulations to determine the potential enzyme that can convert lactate to lactyl-CoA. Next, the histone acetyltransferase p300
(HAT p300) enzyme (PDB ID: 6GYR) was evaluated as a potential enzyme that can bind to lactylCoA during the lactylation process.

Results:
We collected evidence on the secretion of lactate in the extracellular conditioned medium
of breast cancer cells treated by anticancer compositions. MD simulations data projected that acetyl-CoA synthetase could be a potential enzyme that may convert lactate into lactyl-CoA similar to
a known substrate acetate. Furthermore, a specific and efficient binding (docking energy -9.6
kcal/mol) of lactyl-CoA with p300 HAT suggested that lactyl-CoA may serve as a substrate for lactylation similar to acetylation that uses acetyl-CoA as a substrate.

Conclusion:
In conclusion, our data provide a hint on the missing link for the lactylation process
due to lactate in terms of a potential enzyme that can convert lactate into lactyl-CoA. This study
helped us to project the HAT p300 enzyme that may use lactyl-CoA as a substrate in the lactylation
process of the tumor microenvironment.
Current Strategy of Monoclonal Antibody: Development, Cloning,
Formulation and Drug Delivery
Authors: Kaliyaperumal Rajaganapathy , Ranganathan Srinivasan , Krishnamoorthy Masilamani , Mohan Vasanth Kumar , Balaraman Senthilnathan , Rajapandian Gowri , Sathiyasundar Ramalingam , Ravindran Saravanan 
Published: December 2023

Abstract:
The development of Monoclonal antibodies (mAbs) has also allowed researchers to understand
the complexity of diseases better and find new treatments for difficult-to-treat conditions. Using
mAbs, researchers can identify and target specific molecules in the body involved in the disease process.
This has allowed for a more targeted treatment approach, which has resulted in improved outcomes
for many patients. This hypothesis has been the basis for the development of mAbs that can target
an array of illnesses. In the past two decades, therapeutic mAbs have been developed to treat cancer,
autoimmune diseases, cardiovascular diseases, and metabolic diseases. For instance, using mAbs
has improved outcomes in treating rheumatoid arthritis, multiple sclerosis, and Crohn's disease. However,
delivering mAbs in biological systems remains a significant challenge in drug delivery. This is
due to their large size, low stability in circulation, and difficulties in achieving their desired action in
the target cells. Monoclonal antibodies (mAbs) are an essential tool in biological systems, as they can
be used to deliver drugs to specific cell types or tissues. Cloning methods of monoclonal antibody production
have been developed to produce mAbs with therapeutic potential. Hence, the present review
focused on the development and drug delivery of Monoclonal antibodies (mAbs) in biological systems,
which includes cloning methods, various drug delivery technologies, formulation production
technology, and its applications in multiple diseases were focused for this review.
PENGECORAN ALUMINIUM MATRIKS KOMPOSIT BERPENGUAT CARBON NANOTUBE (CNT) DENGAN VARIASI PENAMBAHAN SERBUK MAGNESIUM
Authors: Syahid Muhammad , Elyeser Elyeser , Hayat Azwar 
Published: December 2023

Aluminum matrix composite reinforced CNT have been developed to improve the mechanical properties of aluminum. In this study, the melting of Aluminum 1100 reinforced Carbon Nanotube (1 wt.% CNT) with variations in the addition of Magnesium powder was carried out by the slurry-based method. The casting of the specimens is carried out using a metal mold. CNT powder was first mixed with magnesium powder at 60°C. Then the aluminum is melted to a temperature of 700°C until it melts, then the temperature is lowered to 500°C or the texture has changed to be like mush (slurry based) and then mixed with CNT powder and Magnesium powder are added. After that the alloy was raised again at 720°C for 30 minutes and the stirring process was carried out, after that it was poured into a metal mold with a mold temperature of around 400°C. The samples were then analyzed for mechanical properties and microstructure observations. The results of the Vickers hardness test before the addition of Mg powder and CNT were 36.7 HV. While after the variation of adding 1wt.% Mg powder, the hardness of the specimen increased to 44.5 HV, the variation of adding 3% Mg powder was 45.3 HV, and the variation of adding 5% Mg powder reached a hardness value of 46 HV. The tensile test results on 3% Mg powder is 241.6 MPa. The wear test results show the wear rate value for the 1% Mg variation of 0.146 mg/s, the 3% Mg variation of 0.106 mg/s, and the 5% Mg variation with a wear rate value of 0.093 mg/s. The α-Al phase is the dominant phase formed, where the grain size and grain boundaries in the 3% Mg variation have a relatively small size and the spread of CNT is evenly distributed in the alloy, so the addition of Mg to the alloy can help spread CNT in Aluminum alloys.
Environmental Communication as a Mediator in the Relationship between Environmental Concern and Attitude Towards Green Services in a Sequential Mediation Model: Evidences from Restaurant Industry
Authors: Choudhury Debdutta , Bhattacharjee Anindo 
Published: December 2023

Tourism is a major economic activity of a nation but also contributes to about 5% of global emissions. Hospitality industry, a major arm of tourism industry, has initiated several measures for green sustainable practices. This research aims to understand the linkages between an altruistic mindset, environmental concern, environmental communication, green service attitude, and behavioural intentions through a sequential moderation model in the restaurant industry.
A literature review was done to understand the theoretical construct of the proposed model and extant research for last 30 years to develop the consumer behaviour model. A modified model of Theory of Planned Behaviour was chosen as the base theory and through a review of the extant literature, a hypothetical model was created. A covariance-based structural equation model (CB SEM) was created to test the strength of relationships across the variables. The findings indicate that consumer environmental concern do not directly lead to positive attitude towards green services until it is mediated by environmental communication. This finding will help the green restaurants to adequately design suitable communication strategies to increase consumer awareness, positive attitudes and pro-environmental behavioural intentions.
Impact of CYP2D6 polymorphisms on the efficacy of tamoxifen in Iraqi women with breast cancer
Authors: Sagban Sura , S. Sahib Ahmed , S. Abdulamir Ahmed , M. Kadhim Haitham 
Published: December 2023

Objectives: The aim of the present study was to investigate the impact of the CYP2D6 genetic polymorphism on clinical outcome in Iraqi breast cancer patients who were candidates for Tamoxifen therapy.

Methods: Comprehensive CYP2D6 genotyping was performed in 140 Iraqi women with breast cancer who were women on adjuvant treatment with tamoxifen. Breast cancer patients recruited into the study were divided into two groups: seventy breast cancer women who had no history of recurrence at the time of sampling and had a long time on tamoxifen without recurrence and seventy breast cancer women who had recurrence at the time of sampling after one year of treatment with tamoxifen therapy. Recurrence free survival (RFS) was determined in the recruited patients.

Results: Multiple genetic variants of the gene encoding the CYP2D6 enzyme were detected with significant differences in their frequencies and percentages in both recurrent and non-recurrent groups of breast cancer patients. The findings of this study suggest that interindividual variation in clinical outcome may be related to genetic variation in CYP2D6 enzyme, which is characterized by variable RFS periods.

Conclusions: This study revealed that the CYP2D6 enzyme of breast cancer patients who participated in this study is highly polymorphic. The CYP2D6 gene of study participants exhibited different allelic combinations with variable frequencies. The multiple genetic variants (alleles) of the gene encoding the CYP2D6 enzyme exhibited significant differences in their frequencies and percentages in both recurrent and non-recurrent groups of breast cancer patients. .The study revealed variable Recurrence free survival (RFS) with highly polymorphic gene. Our study quotes the presence of increased CYP2D6 enzyme polymorphism is associated with variable clinical response.
A oralidade como potência no processo de alfabetização: relato de experiência em escola quilombola
Authors: Barbosa Shirley Cristina Amador , Maciel Zilene dos Reis , Silva David Rogerio Santos 
Published: December 2023

Resumo: Neste artigo será apresentado parte da ação desenvolvidas no projeto sob o título “A oralidade como potência no processo de alfabetização de crianças do campo quilombola”, projeto de ensino e extensão do Núcleo de Educação Popular Paulo Freire (NEP), manejado pelo subgrupo - Grupo de Educação Popular com Estudantes Quilombolas (GEPEQ), da Universidade do Estado do Pará (UEPA), que teve como objetivo colaborar com o processo de alfabetização de estudantes quilombolas do 3º ano do ensino fundamental anos iniciais, da Escola Municipal de Educação Infantil e Fundamental Quilombola (EMEIFQ), localizada na comunidade quilombola de Vila União/Campina, em Salvaterra ilha de Marajó/PA. Os aportes metodológicos utilizados foram da abordagem qualitativa e análise descritiva dos encontros ocorridos no projeto. Para tanto, apoiados em Alves e Marques (2021), Rufino (2014), Freire (1988), Arroyo (2014) entre outros que contribuem nas análises deste artigo. Verificou-se que o projeto que fez uso da oralidade e de atividades lúdicas, contribuem na aprendizagem, assimilação dos alunos e são construtores de princípios morais e sociais, como também, tais atividades colaboram no processo de ensino, sendo agregador na aprendizagem.
A recursion formula for the integer power of a symmetric second-order tensor and its application to computational plasticity
Authors: Kouhia Reijo , Saksala Timo 
Published: December 2023

In this paper, a recursion formula is given for the integer power of a second-order tensor in 3D Euclidean space. It can be used in constitutive modelling for approximating failure or yield surfaces with corners, and it is demonstrated for the case of Rankine failure criterion. Removing corners provides clear advantages in computational plasticity. We discuss the consequences of the approximation errors for failure analyses of brittle and quasi-brittlematerials.
Liquid organic matter from banana peel improves morpho-physiological traits of coffee seedlings
Authors: Soleh Mochamad Arief , Zalfa Inaz Nuzul , Ariyanti Mira 
Published: December 2023

Due to its economic advantage, the Robusta coffee clones BP 308 and BP 939 are widely grown. Many factors affect coffee growth during the cultivation practice, especially in the availability of adequate nutrients at the nursery stages. Apart from inorganic fertilizer application, organic matter needs to be applied in the nursery stages to support plant growth, such as applying liquid organic matter (LOM) derived from banana peel waste. This study was conducted to find out how the LOM of banana peels affected the morphological and physiological responses of BP 308 and BP 939 clone Robusta coffee seedlings and what proper dosages of LOM were needed. The experiment was conducted at the Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor from January to April 2022. This experiment used a randomized block design method consisting of 10 treatments and 3 repetitions. The treatments tested included 1.5 g of NPK inorganic fertilizer; 10 mL.L-1.plant-1 LOM ; 20 mL.L-1.plant-1 LOM; 30 mL. L-1.plant-1 LOM and 40 mL. L-1 .plant-1 LOM that was given to both clones, namely BP 308 and BP 939. The results of this experiment showed that there were differences in the effect of LOM between BP308 and BP939 on the morphological response such as an increase in plant height and leaf number, on the physiological response such as chlorophyll index and stomatal conductance at the 3 months after treatment (MAT). The BP939 was partly better in response to LOM of 10 mL.L-1.plant-1on plant height, the BP308 was partly better in stomatal conductance.
Adaptation of several hybrid maize in West Nusa Tenggara drylands using modified plant spacing for optimal seed and biomass productions
Authors: Syahruddin Karlina , Suwardi Suwardi , Priyanto Slamet Bambang , Efendi Roy , Herawati Herawati , Fattah Abdul , Rahman Rahman , Hasbi Hasbi , Aminah Aminah , Fatmawati Fatmawati , Santoso Sigit Budi , Bidhari Lesty Ayu , Abid Muhammad 
Published: December 2023

Maize is a crucial multipurpose strategic food crop in Indonesia. Land expansion employing dry land, row-space technology, and suitable varieties, is emerging as the solution to fulfill the rising need for seeds and biomass. The study was carried out from August to December using a factorial randomized block design consisting of two treatment factors, namely the treatment factor of 6 varieties and 2 row-spacings, to verify the new superior hybrid maize, which is adaptable in dry land Senayan village, Poto Tano sub-district, West Sumbawa district, and West Nusa Tenggara (NTB). The results showed that JH 37 and JH 29 varieties were adaptive to be developed in dry climate dryland areas for seed and biomass production using various narrow and wide planting space system. Jakarin, Bisi 18 and HJ 21 varieties could be planted in drylands by considering the planting space system for seed or biomass production, while the Nasa 29 variety was not recommended to be planted in drylands area for seed production, but could be used for biomass production by considering a wide planting space system such as Legowo system.
An Adaptive PID Control System for the Attitude and Altitude Control of a Quadcopter
Authors: Cedro Leszek , Wieczorkowski Krzysztof , Szcześniak Adam 
Published: December 2023

Abstract
In adaptive model-based control systems, determining the appropriate controller gain is a complex and time-consuming task due to noise and external disturbances. Changes in the controller parameters were assumed to be dependent on the quadcopter mass, which was the process variable. A nonlinear model of the plant was used to identify the mass, employing the weighted recursive least squares (WRLS) method for online identification. The identification and control processes involved filtration using differential filters, which provided appropriate derivatives of signals. Proportional integral derivative (PID) controller tuning was performed using the Gauss–Newton optimisation procedure on the plant. Differential filters played a crucial role in all the developed control systems by significantly reducing measurement noise. The results showed that the performance of classical PID controllers can be improved by using differential filters and gain scheduling. The control and identification algorithms were implemented in an National Instruments (NI) myRIO-1900 controller. The nonlinear model of the plant was built based on Newton’s equations.
Bone mineral density in the various regions of the skeleton in women with subclinical hypothyroidism: the effect of biological factors, bone turnover markers and physical activity
Authors: Kopiczko Anna 
Published: December 2023

Abstract

Study aim: This cross-sectional study examined the relationship between biological factors, physical activity (PA), bone turnover markers (BTMs) and bone mineral density (BMD) in women with subclinical hypothyroidism (SCH) and healthy.

Material and methods: The study included 135 women. Bone parameters were measured by the densitometry. Calcium, phosphorus, osteocalcin (OC), total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), vitamin D and cross-linked carboxyterminal telopeptide of type I collagen (ICTP) were measured in blood serum. PA were evaluated by metabolic equivalent of task (MET).

Results: Women with SCH had lower BMD in hip and lumbar spine, calcium, vitamin D and higher values of ICTP than the healthy group. In women with SCH, the affecting BMD in the femoral neck were ICTP (µg/l), (η² = 0.083), and also 25(OH)D (ng/ml), (η² = 0.080) and PA level (I/S), (η² = 0.115). BMD in the spine was affected by PA level (I/S), (η² = 0.173). The parameters affecting BMC in the femoral neck were 25(OH)D (ng/ml), (η² = 0.073). In all women sufficient levels of PA determined higher BMD.

Conclusions: PA and BTMs significantly determine BMD levels. PA can be an important area of rehabilitation and physical therapy as an available measure to counteract BMD loss in postmenopausal healthy women and those with SCH.
Transitioning from the dental chair to the bronchoscopy table: a rare complication of a root canal procedure
Authors: Hussain Sara , Salih Goran , Mohammadiih Majed , Jalal Michael , AlNajjar Firas 
Published: December 2023

Background:
Dental needle aspiration is a rare complication of oral procedures given the advances in medical technology. Aspirations in general may also be asymptomatic, apart from the possible remote history of upper airway irritation at the time of induction. Clinicians should take a detailed history and examination, along with appropriate diagnostic imaging before discharging these patients from the emergency department.
Case presentation:
A young patient presented with an acute complaint of pain and coughing post-dental procedure. On arrival at the emergency department, she was vitally and clinically stable, without signs of respiratory distress. There were no complaints reported apart from minor throat pain. Imaging done in the emergency department revealed the presence of a 32 mm needle in the distal airway. She underwent emergency flexible bronchoscopy, and the foreign body was removed under direct visualization. The patient remained stable throughout the procedure and had an uneventful stay until discharge.
Conclusion:
While needle aspiration is a relatively uncommon occurrence, its early detection is always critical before further damage to local structures can occur. Regardless of presentation and symptoms, basic imaging is mandatory before the disposition is decided.
Selçuk University Museum Interior Design and Application Example
Authors: Akçaova Ali , Noraslı Mehmet 
Published: December 2023

The aim of this study is to cover the design-implementation processes of the museum interiors of the museum building in Selçuk University campus, which consists of foyer, temporary and permanent exhibition areas that can realize the aims and functions of the museum in accordance with the culture of the institution to which it is affiliated. Two types of materials were used within the scope of the study. The first type of material is theoretical and visual information based publications, thesis studies, lecture notes, architectural and interior design printed publications and internet resources. The information scanned and analyzed was handled from general to specific. The second material is the analysis of the Selçuk University Museum building located in Selçuk University Alaeddin Keykubat Campus by the authors with the on-site identification stages, interviews with the relevant people, the requirements of the museum foyer, temporary, permanent exhibition areas and existing interior photographs. The method followed in the study is observation and detection. As a result of the researches and observations made by utilizing publications, thesis studies, course notes, architectural and interior architectural printed publications and internet resources on the subject, an identification study was carried out in the existing museum building. As a result of the research, it was stated that the museums within the university campus have an important place in terms of establishing a bond between the public and the university, apart from the educational areas affiliated to the institution. Apart from education and training structures, from a different perspective, it was mentioned that they are common multi-purpose areas and the positive aspects they add to the institution to which they are affiliated. The interior revisions made in the determined areas of the museum building, the project design process and the implementation phases were mentioned.