bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of hub genes significantly linked to temporal lobe epilepsy and apoptosis via bioinformatics analysis
Authors: Wang Weiliang , Ren Yinghao , Xu Fei , Zhang Xiaobin , Wang Fengpeng , Wang Tianyu , Zhong Huijuan , Wang Xin , Yao Yi 
Published: February 2024

BackgroundEpilepsy stands as an intricate disorder of the central nervous system, subject to the influence of diverse risk factors and a significant genetic predisposition. Within the pathogenesis of temporal lobe epilepsy (TLE), the apoptosis of neurons and glial cells in the brain assumes pivotal importance. The identification of differentially expressed apoptosis-related genes (DEARGs) emerges as a critical imperative, providing essential guidance for informed treatment decisions.MethodsWe obtained datasets related to epilepsy, specifically GSE168375 and GSE186334. Utilizing differential expression analysis, we identified a set of 249 genes exhibiting significant variations. Subsequently, through an intersection with apoptosis-related genes, we pinpointed 16 genes designated as differentially expressed apoptosis-related genes (DEARGs). These DEARGs underwent a comprehensive array of analyses, including enrichment analyses, biomarker selection, disease classification modeling, immune infiltration analysis, prediction of miRNA and transcription factors, and molecular docking analysis.ResultsIn the epilepsy datasets examined, we successfully identified 16 differentially expressed apoptosis-related genes (DEARGs). Subsequent validation in the external dataset GSE140393 revealed the diagnostic potential of five biomarkers (CD38, FAIM2, IL1B, PAWR, S100A8) with remarkable accuracy, exhibiting an impressive area under curve (AUC) (The overall AUC of the model constructed by the five key genes was 0.916, and the validation set was 0.722). Furthermore, a statistically significant variance (p &lt; 0.05) was observed in T cell CD4 naive and eosinophil cells across different diagnostic groups. Exploring interaction networks uncovered intricate connections, including gene-miRNA interactions (164 interactions involving 148 miRNAs), gene-transcription factor (TF) interactions (22 interactions with 20 TFs), and gene-drug small molecule interactions (15 interactions involving 15 drugs). Notably, IL1B and S100A8 demonstrated interactions with specific drugs.ConclusionIn the realm of TLE, we have successfully pinpointed noteworthy differentially expressed apoptosis-related genes (DEARGs), including CD38, FAIM2, IL1B, PAWR, and S100A8. A comprehensive understanding of the implications associated with these identified genes not only opens avenues for advancing our comprehension of the underlying pathophysiology but also bears considerable potential in guiding the development of innovative diagnostic methodologies and therapeutic interventions for the effective management of epilepsy in the future.
A long-term dependable and reliable method for reactor accident prognosis using temporal fusion transformer
Authors: Li Chengyuan , Li Meifu , Qiu Zhifang 
Published: February 2024

Introduction: The accurate prognosis of reactor accidents is essential for deploying effective strategies that prevent radioactive releases. However, research in the nuclear sector is limited. This paper introduces a novel Temporal Fusion Transformer (TFT) model-based method for accident prognosis that incorporates multi-headed self-attention and gating mechanisms.Methods: Our proposed method combines multi-headed self-attention and gating mechanisms of TFT with multiple covariates to enhance prediction accuracy. Additionally, we employ quantile regression for uncertainty assessment. We apply this method to the HPR1000 reactor to predict outcomes following loss of coolant accidents (LOCAs).Results: The experimental results reveal that our proposed method outperforms existing deep learning-based prediction models in both prediction accuracy and confidence intervals. We also demonstrate increased robustness through interference experiments with varying signal-to-noise ratios and ablation studies on static covariates.Discussion: Our method contributes to the development of intelligent and reduced-staff maintenance methods for reactor systems, showcasing its ability to effectively extract and utilize features of static and historical covariates for improved predictive performance.
Measurement of body composition in postpartum South African women living with and without HIV infection
Authors: Madlala Hlengiwe P. , Myer Landon , Geffen Hayli , Meyer Demi , Mendham Amy E. , Goedecke Julia H. , Bengtson Angela M. , Jao Jennifer , Dugas Lara R. 
Published: February 2024

BackgroundWhile several methodologies are available to measure adiposity, few have been validated in sub-Saharan African (SSA) and none in postpartum African women living with HIV (WLHIV). We compared bioelectrical impendence analysis (BIA) and air displacement plethysmography (ADP) against dual x-ray absorptiometry (DXA) in South African women and examined differences by HIV and body mass index (BMI) status.MethodsLin’s concordance correlation coefficient (CCC) test was used to examine fat mass (FM), fat free mass (FFM), and total body fat percent (%BF) difference between BIA vs. DXA, and ADP vs. DXA in women living with HIV (n = 57) and without HIV (n = 25). The Bland Altman test was used to assess mean differences and the direction of bias.ResultsThe median age was 31 years (IQR, 26–35) and months postpartum were 11 (IQR, 7–16), 44% of the women had obesity. Lin’s CCC for BIA and ADP vs. DXA were both 0.80 for %BF and 0.97 for FM, and 0.86 and 0.80 for FFM, respectively. Mean differences (DXA-BIA and ADP estimates) were 0.22 ± 4.54% (p = 0.54) and 3.35 ± 3.27% (p &lt; 0.01) for %BF, −0.82 ± 3.56 kg (p = 0.06) and 1.43 ± 2.68 kg (p = 0.01) for FM, −1.38 ± 3.61 kg (p = 0.01) and − 3.34 ± 2.37 kg (p &lt; 0.01) for FFM, respectively. BIA overestimated %BF in WLHIV and underestimated it in women with obesity.ConclusionBody composition measurements using BIA and ADP correlated well with DXA, thereby providing alternative, safe tools for measuring postpartum FM and FFM in SSA women, including WLHIV.
Geriatric nutritional risk index as a predictor for postoperative complications in patients with solid cancers: a meta-analysis
Authors: Liu Weichen , Li Ming , Lian Siqin , Hou Xijie , Ling Ying 
Published: February 2024

BackgroundThe geriatric nutritional risk index (GNRI) has been wildly used to predict the prognosis of patients with solid cancer, but it’s value in postoperative complications remains unclear. The aim of our study was to systematically explore the value of the GNRI in postoperative complications in patients with solid cancer.MethodThe study conducted a systematic literature search using electronic databases to investigate the influence of the GNRI on postoperative complications in patients with solid cancer. The search covered articles published up until May 2023. The odds ratio (OR) with a 95% confidence interval (CI) was employed to assess the effect of GNRI on postoperative complications.ResultA total of 11 studies with 11,002 patients were enrolled in our meta-analysis. The results suggested that patients with a low GNRI have a higher risk of experiencing postoperative complications (OR=2.51, 95%CI 2.05–3.02, z=9.86, p&lt;0.001), a higher risk of suffering Clavien-Dindo (CD) grades≥2 complications(OR=2.24, 95%CI 1.84–2.73, z=8.01, p&lt;0.001), a higher risk of suffering infection (OR=1.85, 95%CI 1.18–2.88, z=2.70, p=0.007) and a higher risk of suffering respiratory complications(OR = 2.94, 95%CI: 1.56-5.55, z=3.31, p=0.001).ConclusionBased on existing evidence, the GNRI was a valuable predictor of postoperative complications in patients with solid cancer.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=434299, identifier CRD42023434299.
First-line chemoimmunotherapy and immunotherapy in patients with non-small cell lung cancer and brain metastases: a registry study
Authors: Brown Lauren Julia , Khou Victor , Brown Chris , Alexander Marliese , Jayamanne Dasantha , Wei Joe , Gray Lauren , Chan Wei Yen , Smith Samuel , Harden Susan , Mersiades Antony , Warburton Lydia , Itchins Malinda , Lee Jenny H. , Pavlakis Nick , Clarke Stephen J. , Boyer Michael , Nagrial Adnan , Hau Eric , Pires da Silva Ines , Kao Steven , Kong Benjamin Y. 
Published: February 2024

IntroductionBrain metastases commonly occur in patients with non-small cell lung cancer (NSCLC). Standard first-line treatment for NSCLC, without an EGFR, ALK or ROS1 mutation, is either chemoimmunotherapy or anti-PD-1 monotherapy. Traditionally, patients with symptomatic or untreated brain metastases were excluded from the pivotal clinical trials that established first-line treatment recommendations. The intracranial effectiveness of these treatment protocols has only recently been elucidated in small-scale prospective trials.MethodsPatients with NSCLC and brain metastases, treated with first-line chemoimmunotherapy or anti-PD-1 monotherapy were selected from the Australian Registry and biObank of thoracic cancers (AURORA) clinical database covering seven institutions. The primary outcome was a composite time-to-event (TTE) outcome, including extracranial and intracranial progression, death, or need for local intracranial therapy, which served as a surrogate for disease progression. The secondary outcome included overall survival (OS), intracranial objective response rate (iORR) and objective response rate (ORR).Results116 patients were included. 63% received combination chemoimmunotherapy and 37% received anti-PD-1 monotherapy. 69% of patients received upfront local therapy either with surgery, radiotherapy or both. The median TTE was 7.1 months (95% CI 5 - 9) with extracranial progression being the most common progression event. Neither type of systemic therapy or upfront local therapy were predictive of TTE in a multivariate analysis. The median OS was 17 months (95% CI 13-27). Treatment with chemoimmunotherapy was predictive of longer OS in multivariate analysis (HR 0.35; 95% CI 0.14 – 0.86; p=0.01). The iORR was 46.6%. The iORR was higher in patients treated with chemoimmunotherapy compared to immunotherapy (58% versus 31%, p=0.01). The use of chemoimmunotherapy being predictive of iORR in a multivariate analysis (OR 2.88; 95% CI 1.68 - 9.98; p=0.04).ConclusionThe results of this study of real-world data demonstrate the promising intracranial efficacy of chemoimmunotherapy in the first-line setting, potentially surpassing that of immunotherapy alone. No demonstrable difference in survival or TTE was seen between receipt of upfront local therapy. Prospective studies are required to assist clinical decision making regarding optimal sequencing of local and systemic therapies.
Predicting the recurrence of usual-type cervical adenocarcinoma using a nomogram based on clinical and pathological factors: a retrospective observational study
Authors: Liu Yuting , Zhang Ningning , Yang Qing 
Published: February 2024

BackgroundUsual-type cervical adenocarcinoma is the most frequent type of adenocarcinoma, and its prevalence is increasing worldwide. Tumor recurrence is the leading cause of mortality; therefore, recognizing the risk factors for cervical cancer recurrence and providing effective therapy for recurrent cervical cancer are critical steps in increasing patient survival rates. This study aimed to retrospectively analyze the clinicopathological data of patients with usual-type cervical adenocarcinoma by combining the diagnosis and treatment records after the initial treatment and recurrence.MethodsWe retrospectively analyzed patients diagnosed with usual-type cervical adenocarcinoma who underwent radical hysterectomy and pelvic lymph node dissection at Shengjing Hospital of China Medical University between June 2013 and June 2022. We constructed a nomogram-based postoperative recurrence prediction model, internally evaluated its efficacy, and performed internal validation.ResultsThis study included 395 participants, including 87 individuals with recurrence. At a 7:3 ratio, the 395 patients were divided into two groups: a training set (n = 276) and a validation set (n = 119). The training set was subjected to univariate analysis, and the risk variables for recurrence included smoking, ovarian metastasis, International Federation of Gynaecology and Obstetrics (FIGO) staging, lymphovascular space invasion, perineural invasion, depth of muscular invasion, tumor size, lymph node metastasis, and postoperative HPV infection months. The aforementioned components were analyzed using logistic regression analysis, and the results showed that the postoperative HPV infection month, tumor size, perineural invasion, and FIGO stage were independent risk factors for postoperative recurrence (p&lt;0.05). The aforementioned model was represented as a nomogram. The training and validation set consistency indices, calculated using the bootstrap method of internal validation, were 0.88 and 0.86, respectively. The model constructed in this study predicted the postoperative recurrence of usual-type cervical cancer, as indicated by the receiver operating characteristic curve. The model demonstrated good performance, as evidenced by the area under the curve, sensitivity, and specificity values of 0.90, 0.859, and 0.844, respectively.ConclusionBased on the FIGO staging, peripheral nerve invasion, tumor size, and months of postoperative HPV infection, the predictive model and nomogram for postoperative recurrence of usual-type cervical adenocarcinoma are precise and effective. More extensive stratified evaluations of the risk of cervical adenocarcinoma recurrence are still required, as is a thorough assessment of postoperative recurrence in the future.
The effect of music on pain management in preterm infants during daily painful procedures: a systematic review and meta-analysis
Authors: Ou Yiran , Chen Ling , Zhu Xinyue , Zhang Tianci , Zhou Ye , Zou Lu , Gao Yun , Wang Zhenghao , Zheng Xiaofeng 
Published: February 2024

BackgroundThe present systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to investigate the effects of music on pain management in preterm neonates during painful procedures.MethodsThe PubMed, Embase, Web of Science, EBSCO and Cochrane Library databases were searched to identify relevant articles published from their inception to September 2023. The study search strategy and all other processes were implemented in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement.ResultsFour RCTs that satisfied the inclusion criteria were included in this meta-analysis. The music group had significantly lower Premature Infant Pain Profile (PIPP) scores during (RR = −1.21; 95% CI = −2.02–−0.40, p = 0.0032) and after painful procedures (RR = −0.65; 95% CI = −1.06–−0.23, p = 0.002). The music group showed fewer changes in PIPP scores after invasive operations than did the control group (RR = −2.06; 95% CI −3.16–−0.96; p = 0.0002). Moreover, our results showed that music improved oxygen saturation during (RR = 3.04, 95% CI = 1.64–4.44, p &lt; 0.0001) and after painful procedures (RR = 3.50, 95% CI = 2.11–4.90, p &lt; 0.00001). However, the change in peak heart rate during and after painful procedures was not statistically significant (RR = −12.14; 95% CI = −29.70−5.41 p = 0.18; RR = −10.41; 95% CI = −22.72−1.90 p = 0.10).ConclusionIn conclusion, this systematic review demonstrated that music interventions are effective for relieving procedural pain in preterm infants. Our results indicate that music can reduce stress levels and improve blood oxygen saturation. Due to the current limitations, large-scale, prospective RCTs should be performed to validate the present results.
Overexpression of CXCL17 increases migration and invasion of A549 lung adenocarcinoma cells
Authors: Koni Ekin , Congur Irem , Tokcaer Keskin Zeynep 
Published: February 2024

Lung cancer is one of the most frequently diagnosed malignancies and is a widespread disease that affects millions of individuals globally. CXCL17 is a member of the CXC chemokine family that attracts myeloid cells and is associated with the mucosa. CXCL17 can both support and suppress tumor growth in certain types of cancer. A549 LUAD cells were transfected with N-Terminal p3XFLAG-CMV or N-Terminal p3XFLAG-CMV-CXCL17 to establish stably transfected CXCL17-overexpressing cells. Reverse-transcription polymerase chain reaction (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA) were performed to verify the levels of CXCL17 mRNA and of CXCL17 protein concentration of stably transfected A549 cells respectively. Wound healing, CCK8, and matrigel invasion assays were performed to assess the effect of CXCL17 overexpression on migration, proliferation, and invasion of A549 cells. When compared to control groups, proliferative capacity of A549 cells were unaffected by CXCL17 overexpression; however, the wound area in the CXCL17 overexpression group had dramatically decreased after 48 h. Similarly, the number of invasion cells was significantly higher in the CXCL17-overexpressing group than in the control ones after 48 h. CXCL17 overexpression significantly increased the ability of A549 cells to migrate and invade, without affecting their proliferative abilities.
Interplanetary shock induced intensification of electron cyclotron harmonic waves in the Earth’s inner magnetosphere
Authors: Xie Yi , Liu Nigang , Su Zhenpeng , Yi Siyang , He Zhaoguo , Yu Jiang , Li Kun , Chen Zuzheng , Cui Jun 
Published: February 2024

Electron cyclotron harmonic (ECH) waves are electrostatic emissions frequently observed in the Earth’s magnetosphere. By precipitating magnetospheric hot electrons into the ionosphere, ECH waves play a critical role in the formation of diffuse aurora. Previous research has extensively investigated the strong dependence of ECH waves on the geomagnetic activities. In this study, we present the first report of the prompt response of ECH waves to an interplanetary shock on the basis of WIND and Van Allen Probes observations. Our observations and analyses demonstrate that the interplanetary shock compression can increase >0.1 keV hot electron fluxes in the dayside inner magnetosphere, consequently leading to the prompt intensification of ECH waves by promoting the wave instability. These findings expand our comprehension of the impacts of solar wind disturbances on magnetospheric plasma waves and offer fresh insights into solar wind-magnetosphere-ionosphere coupling.
Baselining physiological parameters in three muscles across three equine breeds. What can we learn from the horse?
Authors: Vidal Moreno de Vega Carmen , de Meeûs d’Argenteuil Constance , Boshuizen Berit , De Mare Lorie , Gansemans Yannick , Van Nieuwerburgh Filip , Deforce Dieter , Goethals Klara , De Spiegelaere Ward , Leybaert Luc , Verdegaal Elisabeth-Lidwien J.M.M. , Delesalle Cathérine 
Published: February 2024

Mapping-out baseline physiological muscle parameters with their metabolic blueprint across multiple archetype equine breeds, will contribute to better understanding their functionality, even across species.Aims: 1) to map out and compare the baseline fiber type composition, fiber type and mean fiber cross-sectional area (fCSA, mfCSA) and metabolic blueprint of three muscles in 3 different breeds 2) to study possible associations between differences in histomorphological parameters and baseline metabolism.Methods: Muscle biopsies [m. pectoralis (PM), m. vastus lateralis (VL) and m. semitendinosus (ST)] were harvested of 7 untrained Friesians, 12 Standardbred and 4 Warmblood mares. Untargeted metabolomics was performed on the VL and PM of Friesian and Warmblood horses and the VL of Standardbreds using UHPLC/MS/MS and GC/MS. Breed effect on fiber type percentage and fCSA and mfCSA was tested with Kruskal-Wallis. Breeds were compared with Wilcoxon rank-sum test, with Bonferroni correction. Spearman correlation explored the association between the metabolic blueprint and morphometric parameters.Results: The ST was least and the VL most discriminative across breeds. In Standardbreds, a significantly higher proportion of type IIA fibers was represented in PM and VL. Friesians showed a significantly higher representation of type IIX fibers in the PM. No significant differences in fCSA were present across breeds. A significantly larger mfCSA was seen in the VL of Standardbreds. Lipid and nucleotide super pathways were significantly more upregulated in Friesians, with increased activity of short and medium-chain acylcarnitines together with increased abundance of long chain and polyunsaturated fatty acids. Standardbreds showed highly active xenobiotic pathways and high activity of long and very long chain acylcarnitines. Amino acid metabolism was similar across breeds, with branched and aromatic amino acid sub-pathways being highly active in Friesians. Carbohydrate, amino acid and nucleotide super pathways and carnitine metabolism showed higher activity in Warmbloods compared to Standardbreds.Conclusion: Results show important metabolic differences between equine breeds for lipid, amino acid, nucleotide and carbohydrate metabolism and in that order. Mapping the metabolic profile together with morphometric parameters provides trainers, owners and researchers with crucial information to develop future strategies with respect to customized training and dietary regimens to reach full potential in optimal welfare.
Risk perceptions of COVID-19 in Beijing: a cross-sectional study
Authors: Liu Qing , Tan Yiyang , Zhu Zheng , Zhang Jiawei , Fu Yaqun , Wang Quan , Nie Zhijie , Yang Li , Li Xiaoguang 
Published: February 2024

BackgroundThe Chinese government has ended the “dynamic zero-COVID” policy, and residents are now living together with the SARS-CoV-2 virus. Only a limited number of studies have investigated the specific content and structure of COVID-19-related risk perceptions, as well as their underlying determinants. This study measured the residents’ risk perception of COVID-19 and analyzed the predictors of RP.MethodsWe conducted a comprehensive questionnaire-based survey among residents mostly in Beijing, using a specially designed scale consisting of 11 items to accurately measure COVID-19 risk perceptions. We then utilized multiple linear regression analysis to investigate the factors associated with risk perceptions.ResultsA total of 60,039 residents participated in the survey. Our study reveals that COVID-19-related worries are significantly influenced by other dimensions of RP (p &lt; 0.001), except for perceived society’s control of the epidemic. Several experiential and socio-demographic factors, including gender, educational level, and infectious experience, are notably correlated with all dimensions of risk perceptions of COVID-19.ConclusionThis study evaluates the specific content and structure of COVID-19-related risk perceptions, as well as their determinants. It is essential to understand the risk perceptions and health-protective behaviors of residents with diverse educational levels, incomes, and medical histories.