bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ultrasound combined with pineapple protease treatment on the tenderness of dried shrimp
Authors: Zhou Xinyi , Wang Jian , Zhao Jingxu , Yuan Chunhong , Zhang Xiaojun , Huang Tao , Yang Wenge , Wei Huamao 
Published: January 2024

AbstractBACKGROUNDIn order to improve the tenderness of dried shrimp products as well as to reduce the hardness of the meat during the drying process, shrimp were treated with ultrasound combined with pineapple protease and the tenderization condition was optimized by measuring the texture and shear force of dried shrimp. In addition, the sulfhydryl content, myofibril fragmentation index (MFI) and microstructure were also examined to clarify the mechanisms of shrimp tenderization.RESULTSThe results showed UB1 group with ultrasonic power of 100 W, heating temperature of 50 °C and pineapple protease concentration of 20 U mL−1 were the optimum tenderization conditions, where shrimp showed the lowest hardness (490.76 g) and shear force (2006.35 gf). Microstructure as well as sodium dodecyl sulfate–polyacrylamide gel electrophoresis results suggested that during the tenderization process the muscle segments of shrimps were broken, degradation of myofibrillar proteins occurred, and MFI values and total sulfhydryl content increased significantly (P < 0.05) (MFI value = 193.6 and total sulfhydryl content = 93.93 mmol mg−1 protein for UB 1 group).CONCLUSIONUltrasound combined with bromelain could be used as a simple and effective tenderization method for the production of tender dried shrimp. The best conditions were 100 W ultrasonic power, 50 °C ultrasonic temperature, and 20 U mL−1 bromelain. © 2024 Society of Chemical Industry.
Pediatric Tracheotomy Stomal Maturation and Tracheocutaneous Fistulas
Authors: Ha Tu‐Anh N. , Jain Samagra , Schuman Ari , Ongkasuwan Julina 
Published: January 2024

ObjectiveThe purpose of this study is to determine whether tracheostomy stomal maturation affects the risk of tracheocutaneous fistula (TCF) in children.MethodsA retrospective chart review was conducted for all children who both underwent a tracheostomy and were decannulated between 2012 and 2021 at a tertiary children's hospital. Charts were analyzed for demographics, surgical technique, and development of a TCF. TCF was defined as a persistent fistula following 3 months after decannulation.Results179 children met inclusion criteria. The median (interquartile range) age at tracheostomy was 1.5 (82.4) months, average (standard deviation [SD]) duration of tracheotomy was 20.0 (20.6) months, and length of follow‐up after decannulation (range; SD) was 39.3 (4.4–110.0; 26.7) months. 107 patients (60.0%) underwent stomal maturation and 98 patients developed a TCF (54.7%).Younger age at tracheostomy placement was significantly associated with increased risk of TCF, mean (SD) age 28.4 (51.4) version 80.1 (77.5) months (p < 0.001). Increased duration of tracheostomy was significantly associated with increased risk of TCF, 27.5 (18.4) version 11.0 (18.2) months (p < 0.001).Stomal maturation was not significantly associated with the risk of TCF, including on multivariable analysis adjusting for age at tracheostomy and duration of tracheostomy (p = 0.089).ConclusionTracheostomy stomal maturation did not affect the risk of TCF in children, even after adjusting for age and duration of tracheostomy.Level of Evidence4 Laryngoscope, 134:2941–2944, 2024
Partial Ossicular Reconstruction With a Novel Ball Joint Prosthesis: The mCLIP ARC Partial Prosthesis
Authors: Bevis Nicholas , Effertz Thomas , Hüser Marc , Beutner Dirk 
Published: January 2024

ObjectiveMiddle ear surgery involves reconstruction of the ossicular chain, predominately using rigid implants. New middle ear prostheses strive to mimic the physiologic micromovements of the ossicular chain and prevent dislocation, protrusion, and preloading of the annular ligament due to pressure fluctuations.MethodsThirty‐five patients were included in a monocentric, prospective observational study. Patients received tympanoplasty with ossicular reconstruction using the mCLIP ARC partial prosthesis. This titanium prosthesis is equipped with a clip mechanism for coupling at the stapes and a ball joint connecting headplate and shaft. At short‐term (ST) and mid‐term (MT) follow‐up, pure‐tone audiometry was performed and the pure tone average of 0.5, 1, 2, and 3 kHz (PTA4) was calculated. The audiological outcome was compared with retrospective data of the Dresden titanium clip prosthesis.ResultsThe new prosthesis shows favorable clinical results. Pure‐tone audiometry showed satisfactory results in ST and MT follow‐up, with the PTA4 air‐bone gap (ABG) decreasing from 24.5 (±11) dB to 17.4 (±7.9) dB at the ST follow‐up at 27 days to 15.6 (±10.3) dB at MT follow‐up at 196 days (n = 32). A PTA4‐ABG value of less than 20 dB was achieved by 63% of patients at ST follow‐up and by 77% at MT follow‐up. There was no significant difference in PTA4 ABG compared to the Dresden titanium clip prosthesis during ST follow‐up (p = 0.18).ConclusionThe mCLIP ARC partial prosthesis, a new middle ear prosthesis with a balanced ball joint, shows promising audiological results and is a safe and effective choice for patients with chronic ear disease.Level of Evidence3 Laryngoscope, 134:3323–3328, 2024
Recovery of Recurrent Laryngeal Nerve Function With Neoadjuvant Treatment: Neural Characterization
Authors: Russell Marika D. , Abdelhamid Ahmed Amr H. , Feng Zipei , Shonka David C. , Karcioglu Amanda S. , Iwata Ayaka J. , Kyriazidis Natalia , Siddiqui Sameer H. , Athni Tejas S. , Park Jong C. , Wirth Lori J. , Zafereo Mark E. , Randolph Gregory W. 
Published: January 2024

ObjectivesNeoadjuvant targeted therapy has emerged as a promising treatment strategy for locally aggressive thyroid cancer. Its impact on tumor and adjacent tissues remains a nascent area of study. Here we report on a series of six subjects with locally advanced thyroid cancer and recurrent laryngeal nerve (RLN) paralysis who experienced recovery of RLN function with neoadjuvant treatment and describe the morphologic and electrophysiologic characteristics of these recovered nerves.MethodsThis is a multicenter retrospective review. Descriptive analysis was conducted to examine the following parameters for recovered nerves: (1) nerve morphology, characterized as Type A (involving epineurium only) versus Type B (extending beyond epineurium); (2) proximal stimulability (normal vs. abnormal vs. absent); and (3) surgical management (resection vs. preservation).ResultsSix subjects with unilateral VFP were identified. Median time to return of VF mobility was 3 months (range 2–13.5). All nerves (100%) were noted to have Type A morphology at surgery. Proximal stimulability was normal in four subjects (66.7%), abnormal in one (16.7%), and absent in one (16.7%). Nerves that had improvement of function through neoadjuvant therapy were able to be surgically preserved in five subjects (83.3%).ConclusionsThis represents the first characterization of RLNs that have recovered function with neoadjuvant treatment of locally advanced thyroid cancer. Although much remains unknown, our findings indicate carcinomatous neural invasion is a reversible process and recovered nerves may demonstrate normal morphology and electrophysiologic activity.Level of Evidence4 Laryngoscope, 134:3415–3419, 2024
The effect of transfusion on survival in head and neck cancer after free tissue reconstruction
Authors: Patil Yash J. , Yakoub Mohamed , Moreno Kattia F. , Cotton Colin , Tabangin Meredith E. , Altaye Mekibib , Patil Reena Dhanda , Tang Alice , Zender Chad , Domack Aaron 
Published: January 2024

AbstractObjectiveTo examine if perioperative blood transfusion affects overall survival (OS) and recurrence‐free survival (RFS) in head and neck cancer patients who undergo free tissue reconstruction.DesignRetrospective cohort study.MethodsThe medical records of free tissue flaps between 2007 and 2010 were reviewed. Differences in demographics and clinical factors based on the level of transfused packed red blood cells (PRBC) were examined using chi‐squared tests, Kruskal–Wallis tests, and/or ANOVA tests. Survival time was compared using a Cox proportional hazard model.ResultsData were available for 183 patients. Patients who had PRBC transfusion significantly differed from the non‐transfused group by flap type, flap with bone, Charlson Comorbidity Index (CCI), and hemoglobin and hematocrit. When stratified into three groups based on units of PRBC; flap type, flap with bone, CCI, preoperative hemoglobin, and hematocrit were found to differ significantly. The 2‐year Kaplan–Meier plot demonstrated improved OS for those who did not receive any PRBC transfusion. The use of more than 3 units of blood decreased 2‐year OS significantly when compared to the non‐transfused group. Finally, after adjusting for CCI using a Cox proportional hazard model, survival was significantly affected by CCI.ConclusionAfter controlling for patient age, oncologic stage, cancer subsite, histology, type of free flap, vascularized bone‐containing flap, recurrence type, CCI, and preoperative hemoglobin and hematocrit, patients who received 3 or more units of PRBC in the perioperative period had significantly decreased OS. RFS did not differ between the transfused versus non‐transfused groups.Level of EvidenceLevel 4.
Evaluation of a self‐optimizing local mold temperature control for inline warpage reduction of injection molded parts
Authors: Hopmann C. , Kahve C. E. , Fritsche D. C. , Fellerhoff J. 
Published: January 2024

AbstractDimensional accuracy is a highly important quality criterion for injection molded parts and due to the decreasing tolerance criteria an ongoing issue for mold makers, part designers and manufacturers. Variations in the molding process regarding temperature, pressure and thus the specific volume are leading to inner stress distributions within the part, which ultimately cause warpage. To reduce warpage, a homogenization of the local melt properties, such as the temperature, cooling rate or specific volume is necessary. To be able to manipulate these melt properties locally, a mold with a highly segmented temperature control, consisting of 18 heating ceramics and carbon dioxide expansion chambers was designed. This work seeks to reduce warpage using a newly developed control loop that adjusts target values for the upcoming molding cycle while considering the control error of the previous cycle. The self‐optimizing control loop‐ aiming to homogenize the local mold temperatures over multiple cycles‐ was evaluated in experimental trials. Test specimens were produced, and measured for warpage. Compared to the reference (no control), the warpage of a plate‐shaped geometry was reduced by 10 % with the mold temperature homogenization approach.
Ray tracing method with implicit surface detection for smoothed particle hydrodynamics‐based laser beam welding simulations
Authors: Westhofen L. , Kruska J. , Bender J. , Warkentin S. , Mokrov O. , Sharma R. , Reisgen U. 
Published: January 2024

AbstractAn important prerequisite for process simulations of laser beam welding is the accurate depiction of the surface energy distribution. This requires capturing the optical effects of the laser beam occurring at the free surface. In this work, a novel optics ray tracing scheme is proposed which can handle the reflection and absorption dynamics associated with laser beam welding. Showcasing the applicability of the approach, it is coupled with a novel surface detection algorithm based on smoothed particle hydrodynamics (SPH), which offers significant performance benefits over reconstruction‐based methods. The results are compared to state‐of‐the‐art experimental results in laser beam welding, for which an excellent correspondence in the case of the energy distributions inside capillaries is shown.
Identification of a complex intrachromosomal inverted insertion in the long arm of chromosome 9 as a cause of tuberous sclerosis complex in a Korean family
Authors: Ryu Seung Woo , Yoon Ji‐Hee , Kim Dong‐wook , Han Beomman , Han Heonjong , Han Joohyun , Lee Hane , Seo Go Hun , Lee Beom Hee 
Published: January 2024

AbstractBackgroundTuberous sclerosis complex (TSC) is an autosomal dominant multisystem disorder, caused by a loss‐of‐function of either TSC1 or TSC2 gene. However, in 10%–15% TSC patients there is no pathogenic variant identified in either TSC1 or TSC2 genes based on standard clinical testing.MethodsIn this study, genome sequencing was performed for families with clinical diagnosis of TSC with negative results from TSC1 and TSC2 single‐gene tests.ResultsHerein, we report a family presenting a classical TSC phenotype with an unusual, complex structural variant involving the TSC1 gene: an intrachromosomal inverted insertion in the long arm of chromosome 9. We speculate that the inverted 9q33.3q34.13 region was inserted into the q31.2 region with the 3′‐end of the breakpoint of the inversion being located within the TSC1 gene, resulting in premature termination of TSC1.ConclusionsIn this study, we demonstrate the utility of genome sequencing for the identification of complex chromosomal rearrangement. Because the breakpoints are located within the deep intronic/intergenic region, this copy‐neutral variant was missed by the TSC1 and TSC2 single‐gene tests and contributed to an unknown etiology. Together, this finding suggests that complex structural variants may be underestimated causes for the etiology of TSC.
Deletion of the Sodium Glucose Cotransporter 1 (Sglt‐1) impairs mouse sperm movement
Authors: Numata September , Oishee Mumtarin Jannat , McDermott Jeffrey , Koepsell Hermann , Vallon Volker , Blanco Gustavo 
Published: January 2024

AbstractThe Sodium Glucose Cotransporter Isoform 1 (Sglt‐1) is a symporter that moves Na+ and glucose into the cell. While most studies have focused on the role of Sglt‐1 in the small intestine and kidney, little is known about this transporter's expression and function in other tissues. We have previously shown that Sglt‐1 is expressed in the mouse sperm flagellum and that its inhibition interferes with sperm metabolism and function. Here, we further investigated the importance of Sglt‐1 in sperm, using a Sglt‐1 knockout mouse (Sglt‐1 KO). RNA, immunocytochemistry, and glucose uptake analysis confirmed the ablation of Sglt‐1 in sperm. Sglt‐1 KO male mice are fertile and exhibit normal sperm counts and morphology. However, Sglt‐1 null sperm displayed a significant reduction in total, progressive and other parameters of sperm motility compared to wild type (WT) sperm. The reduction in motility was exacerbated when sperm were challenged to swim in media with higher viscosity. Parameters of capacitation, namely protein tyrosine phosphorylation and acrosomal reaction, were similar in Sglt‐1 KO and WT sperm. However, Sglt‐1 KO sperm displayed a significant decrease in hyperactivation. The impaired motility of Sglt‐1 null sperm was observed in media containing glucose as the only energy substrate. Interestingly, the addition of pyruvate and lactate to the media partially recovered sperm motility of Sglt‐1 KO sperm, both in the low and high viscosity media. Altogether, these results support an important role for Sglt‐1 in sperm energetics and function, providing sperm with a higher capacity for glucose uptake.
Quantitative MRI reveals heterogeneous impacts of treatment on diseased bone marrow in a mouse model of myelofibrosis
Authors: Robison Tanner H. , Lee Winston , Luker Kathryn E. , Pettit Kristen , Talpaz Moshe , Chenevert Thomas L. , Ross Brian D. , Luker Gary D. 
Published: January 2024

AbstractPurposeAnalyzing bone marrow in the hematologic cancer myelofibrosis requires endpoint histology in mouse models and bone marrow biopsies in patients. These methods hinder the ability to monitor therapy over time. Preclinical studies typically begin treatment before mice develop myelofibrosis, unlike patients who begin therapy only after onset of disease. Using clinically relevant, quantitative MRI metrics allowed us to evaluate treatment in mice with established myelofibrosis.MethodsWe used chemical shift–encoded fat imaging, DWI, and magnetization transfer sequences to quantify bone marrow fat, cellularity, and macromolecular components in a mouse model of myelofibrosis. We monitored spleen volume, the established imaging marker for treatment, with anatomic MRI. After confirming bone marrow disease by MRI, we randomized mice to treatment with an approved drug (ruxolitinib or fedratinib) or an investigational agent, navitoclax, for 33 days. We measured the effects of therapy over time with bone marrow and spleen MRI.ResultsAll treatments produced heterogeneous responses with improvements in bone marrow evident in subsets of individual mice in all treatment groups. Reductions in spleen volume commonly occurred without corresponding improvement in bone marrow. MRI revealed patterns associated with effective and ineffective responses to treatment in bone marrow and identified regional variations in efficacy within a bone.ConclusionsQuantitative MRI revealed modest, heterogeneous improvements in bone marrow disease when treating mice with established myelofibrosis. These results emphasize the value of bone marrow MRI to assess treatment in preclinical models and the potential to advance clinical trials for patients.
Catalytic effect of metal ions on flame‐retardant cellulose paper with montmorillonite through layer‐by‐layer self‐assembly
Authors: Yan Su , Yi Deqi , Lu Hongyu , Huang Le , Xu Xiaoying , Gao Ming , Hao Jianwei 
Published: January 2024

AbstractA simple, environmentally friendly and efficient montmorillonite (MMT)‐metal ion (Me: Mg2+, Ca2+, Fe3+ and Zn2+)‐carboxymethyl cellulose (CMC) organic–inorganic hybrid film is successfully applied to flame retardant cellulose paper through layer‐by‐layer self‐assembly (LbL) technology. Using SEM, TGA, XPS, air permeability test and horizontal flammability test to characterize its surface morphology, thermal stability, air permeability and flame retardancy. The results show that MMT‐Me‐CMC system can endow cellulose paper with high flame retardancy properties, and different metal ions have different contributions to the flame retardancy. MMT with multi‐layer structure and high specific surface area is beneficial to reduce the air permeability of paper, thereby effectively reducing the flammability of flammable pyrolysis gas and the transfer of heat. The XPS analysis shows that the Zn2+ flame retardant system promotes the formation of graphitized char in the condensed phase. Meanwhile, the possible flame‐retardant mechanism of the system is clarified. Zn2+ replaces Al3+ in MMT, thus increasing the active acidic catalytic sites on MMT. This promotes the carbonization of degradation products and forms graphitized clay‐rich char. This work provides a simple and efficient LbL preparation strategy and provides a reference for the catalytic role of metal ions in the flame retardancy of cellulose paper.
Biocompatible nanocomposite scaffolds based on carrageenan incorporating hydroxyapatite and hesperidin loaded nanoparticles for bone tissue regeneration
Authors: Taymouri Somayeh , Hasani Faezeh , Mirian Mina , Farhang Armin , Varshosaz Jaleh 
Published: January 2024

AbstractIn this study, injectable and osteoinductive carrageenan (CGN)‐based composite hydrogel integrated with 1% wt/vol hydroxyapatite (HA) and hesperidin (HPN) loaded poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate acid) nanoparticles (PHBV NPs) were developed as a scaffold for bone tissue engineering. Accordingly, HPN was encapsulated into PHBV NPs by the oil in water emulsion‐solvent evaporation technique and optimized using the fractional factorial design. Then, the effects of the amount of drug, polymer, surfactant concentration, sonication time, and the water/organic phase volume ratio on the characteristics of NPs, including particle size (PS), polydispersity index (PdI), zeta potential (ZP), drug loading (DL) % and encapsulation efficiency (EE) % were investigated. The crystalline state of HPN and the interaction between the drug and the polymer were also investigated using the x‐ray powder diffraction method and the Fourier transform infrared method, respectively. Further, CGN hydrogel was developed and loaded with optimized NPs and HA (HPN‐PHBV NPs‐CGN/HA hydrogel). The prepared hydrogel was then characterized for drug release, swelling and degradation behavior, mechanical properties, and injectability. In addition, the potential of the composite hydrogel as bone tissue scaffolds was assessed by the MTT assay, alkaline phosphatase (ALP) activity, and alizarin red S staining. The optimized formulation had the size of 353.5 nm, PdI of 0.43, EE% of 88.47%, DL% of 29.58% and the ZP of −17.4. HPN‐PHBV NPs‐CGN/HA hydrogel also showed sustained drug release, as compared to the HPN‐CGN/HA hydrogel. Further, the hydrogel containing HA showed improved mechanical properties, as compared with the HA free one. In addition, HPN‐PHBV NPs‐CGN/HA hydrogel significantly enhanced MG63 cell proliferation, ALP activity and matrix mineralization, as compared with other groups. Overall, the use of this hydrogel with enhanced osteogenic capacity could be considered as an effective approach for bone tissue engineering.
Toward implementation of an evidence‐based intervention for fear of cancer recurrence: Feasibility in real‐world psycho‐oncology practice
Authors: Deuning‐Smit Esther , Custers José A. E. , Braam Cheyenne I. W. , Hermens Rosella P. M. G. , Prins Judith B. 
Published: January 2024

AbstractObjectiveFew evidence‐based interventions addressing high levels of fear of cancer recurrence (FCR) have been implemented. Understanding how these might be implemented is crucial to bridge the research‐practice gap. This study investigated the feasibility of implementing the blended Survivors' Worries of Recurrent Disease (SWORD) intervention in real‐world psycho‐oncology practice.MethodsSWORD was offered for 15 months (2021–2022) as the standard care for clinical FCR in a university hospital, a general hospital, and psycho‐oncological center. We evaluated using a mixed‐methods design six feasibility outcomes based on Bowen's framework: demand, limited effectiveness, degree of execution, acceptability, practicality, and integration. Anonymous data were collected for all oncology patients on referral. Study participants completed questionnaires before and after treatment, including the Cancer Worry Scale (CWS‐6) as the primary measure of effectiveness. Qualitative data included interviews with patients and psychologists, and field notes.ResultsRegarding demand, 81 of 644 patients referred (13%) were eligible for SWORD. The uptake of SWORD was 79% (n = 63/80) and the completion rate 73% (n = 46/63). SWORD was effective in reducing FCR (p < 0.001, ηp2 = 0.694). Regarding execution, a variability in the length, planning and number of treatment sessions was found between different settings. Adherence to the treatment manual's content was high (89%). Regarding acceptability, most patients were satisfied with SWORD (average 8.2/10) and psychologists valued the blended format. Psychologists reported SWORD was practical to deliver given their knowledge and skills. Although differences between settings were found, SWORD integrated well into practice. Referral for FCR and a reluctance to contract new eHealth providers were barriers for implementation.ConclusionsDespite differences between healthcare settings, the implementation of SWORD was evaluated well. The feasibility of SWORD in different settings should inform a national implementation strategy.