bluuebunny/crossref_metadata_2025_split huggingface dataset:
Viviparous1 Alters Global Gene Expression Patterns through Regulation of Abscisic Acid Signaling
Authors: Suzuki Masaharu , Ketterling Matthew G. , Li Qin-Bao , McCarty Donald R. 
Published: July 2003

Abstract
Maize (Zea mays) Viviparous1 (VP1) and Arabidopsis ABI3 are orthologous transcription factors that regulate key aspects of plant seed development and ABA signaling. To understand VP1-regulated gene expression on a global scale, we have performed oligomicroarray analysis of transgenic Arabidopsis carrying 35S::VP1 in an abi3 null mutant background. We have identified 353 VP1/ABA-regulated genes by GeneChip analysis. Seventy-three percent of the genes were affected by both VP1 and ABA in vegetative tissues, indicating a tight coupling between ABA signaling and VP1 function. A large number of seed-specific genes were ectopically expressed in vegetative tissue of 35S::VP1 plants consistent with evidence that VP1 and ABI3 are key determinants of seed-specific expression. ABI5, a positive regulator of ABA signaling, was activated by VP1, indicating conservation of the feed-forward pathway mediated by ABI3. ABA induction of ABI1 and ABI2, negative regulators of ABA signaling, was strongly inhibited by VP1, revealing a second pathway of feed-forward regulation. These results indicate that VP1 strongly modifies ABA signaling through feed-forward regulation of ABI1/ABI5-related genes. Of the 32 bZIP transcription factors represented on the GeneChip, genes in the ABI5 clade were specifically coregulated by ABA and VP1. Statistical analysis of 5′ upstream sequences of the VP1/ABA-regulated genes identified consensus abscisic responsive elements as an enriched element, indicating that many of the genes could be direct targets of the ABI5-related bZIPs. The Sph element is an enriched sequence motif in promoters of genes co-activated by ABA and VP1 but not in promoters of genes activated by ABA alone. This analysis reveals that distinct combinatorial patterns of promoter elements distinguish subclasses of VP1/ABA coregulated genes.
Molecular and Biochemical Characterization of VR-EILs Encoding Mung Bean ETHYLENE INSENSITIVE3-LIKE Proteins
Authors: Lee Jae-Hoon , Kim Woo Taek 
Published: July 2003

Abstract
ETHYLENE INSENSITIVE3 (EIN3) is a transcription factor involved in the ethylene signal transduction pathway in Arabidopsis. Two full-length cDNA clones, pVR-EIL1 and pVR-EIL2, encoding EIN3-LIKE proteins were isolated by reverse transcriptase-polymerase chain reaction and by screening the cDNA library of mung bean (Vigna radiata) hypocotyls. VR-EIL1 and VR-EIL2 share 70% identity and display varying degrees of sequence conservation (39%–65%) with previously isolated EIN3 homologs from Arabidopsis, tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum) plants. Gel retardation assay revealed that both VR-EILs were able to interact specifically with optimal binding sequence-1, the recently identified optimal binding sequence for tobacco TEIL, with the binding of VR-EIL2 being more efficient than that of VR-EIL1. Transient expression analysis using a VR-EIL::smGFP fusion gene in onion (Allium cepa) epidermal cells indicated that the VR-EIL proteins were effectively targeted to the nucleus. The fusion protein of VR-EIL2 with GAL4 DNA-binding domain strongly activated transcription of a reporter gene in yeast cells, and an essential domain for transcription-stimulating activity was localized to the amino-terminal acidic region that consists of 50 amino acid residues. In contrast with what has been previously found in EIN3- and TEIL-overexpressing Arabidopsis plants, transgenic tobacco seedlings expressing the VR-EIL genes under the control of cauliflower mosaic virus 35S promoter did not exhibit a constitutive triple response. Instead, they displayed a markedly enhanced proliferation of root hairs, one of the typical ethylene response phenotypes, and increased sensitivity to exogenous ethylene. In addition, the pathogenesis-related (PR) genes encoding β-1,3-glucanase, osmotin, and PR1 were constitutively expressed in 35S::VR-EIL lines without added ethylene, and were hyperinduced in response to ethylene treatment. These results indicate that VR-EILs are functional in tobacco cells, thereby effectively transactivating the GCC-box-containing PR genes and enhancing sensitivity to ethylene. The possible physiological role of VR-EILs is discussed in the light of the suggestion that they are active components of the ethylene-signaling pathway and their heterologous expressions constitutively turn on a subset of ethylene responses in tobacco plants.
The Function of Ascorbate Oxidase in Tobacco
Authors: Pignocchi Cristina , Fletcher John M. , Wilkinson Joy E. , Barnes Jeremy D. , Foyer Christine H. 
Published: July 2003

AbstractThe function of the apoplastic enzyme ascorbate oxidase (AO) was investigated in tobacco (Nicotiana tabacum). The abundance of AO mRNA was up-regulated by light. Cytosolic ascorbate peroxidase (APX1) transcripts were also highest in the light. In contrast, l-galactono-γ-lactone dehydrogenase, stromal APX, and thylakoid APX transcripts remained constant over the day/night cycle. Salicylic acid inhibited growth, increased expression of the pathogenesis-related protein (PR) 1a, and decreased AO transcript abundance. In contrast, the application of auxin enhanced growth and increased AO and PR 1a gene expression. Therefore, AO transcript abundance varied in a manner similar to hormone-mediated changes in plant growth. To study the effects of modified AO expression on growth, transformed tobacco plants expressing AO in the sense and antisense orientations were generated. The resultant large changes in apoplastic AO activity in the transformed tobacco plants had little effect on whole leaf ascorbate (AA) content, but they had dramatic effects on apoplastic AA levels. Enhanced AO activity oxidized the apoplastic AA pool, whereas decreased AO activity increased the amount of AA compared with dehydroascorbate. A relationship was observed between AO activity and plant height and biomass. Native AO transcript levels were no longer subject to light/dark regulation in AO sense and antisense plants. Taken together, these data show that there is an interaction between hormone, redox, and light signals at the level of the apoplast via modulation of ion of AA content.
Regulation of Arabidopsis COPINE 1 Gene Expression in Response to Pathogens and Abiotic Stimuli
Authors: Jambunathan Niranjani , McNellis Timothy W. 
Published: July 2003

Abstract
The copines are a widely distributed class of calcium-dependent, phospholipid-binding proteins of undetermined biological function. Mutation of the Arabidopsis CPN1 (COPINE 1) gene causes a humidity-sensitive lesion mimic phenotype with increased resistance to a bacterial and an oomyceteous pathogen, constitutive pathogenesis-related gene expression, and an accelerated hypersensitive cell death defense response. Here, we show that the disease resistance phenotype of the cpn1-1 mutant was also temperature sensitive, demonstrate increased CPN1 gene transcript accumulation in wild-type plants under low-humidity conditions, and present a detailed analysis of CPN1 gene transcript accumulation in response to bacterial pathogens. In wild-type plants, CPN1 transcript accumulation was rapidly, locally, and transiently induced by both avirulent and virulent strains of Pseudomonas syringae pv tomato bacteria. However, induction of CPN1 transcript accumulation by avirulent bacteria was much faster and stronger than that induced by virulent bacteria. Bacterial induction of CPN1 transcript accumulation was dependent on a functional type III bacterial protein secretion system. In planta expression of the avrRpt2 avirulence gene was sufficient to trigger rapid CPN1 transcript accumulation. CPN1 transcript accumulation was induced by salicylic acid treatment but was not observed during lesion formation in the lesion mimic mutants lsd1 and lsd5. These results are consistent with CPN1 playing a role in plant disease resistance responses, possibly as a suppressor of defense responses including the hypersensitive cell death defense response. The results also suggest that CPN1 may represent a link between plant disease resistance and plant acclimation to low-humidity and low-temperature conditions.
Mitochondrial Phosphatidylserine Decarboxylase from Higher Plants. Functional Complementation in Yeast, Localization in Plants, and Overexpression in Arabidopsis
Authors: Rontein Denis , Wu Wen-I , Voelker Dennis R. , Hanson Andrew D. 
Published: July 2003

Abstract
Plants are known to synthesize ethanolamine (Etn) moieties by decarboxylation of free serine (Ser), but there is also some evidence for phosphatidyl-Ser (Ptd-Ser) decarboxylation. Database searches identified diverse plant cDNAs and an Arabidopsis gene encoding 50-kD proteins homologous to yeast (Saccharomyces cerevisiae) and mammalian mitochondrial Ptd-Ser decarboxylases (PSDs). Like the latter, the plant proteins have putative mitochondrial targeting and inner membrane sorting sequences and contain near the C terminus a Glycine-Serine-Threonine motif corresponding to the site of proteolysis and catalytic pyruvoyl residue formation. A truncated tomato (Lycopersicon esculentum) cDNA lacking the targeting sequence and a chimeric construct in which the targeting and sorting sequences were replaced by those from yeast PSD1 both complemented the Etn requirement of a yeast psd1 psd2 mutant, and PSD activity was detected in the mitochondria of the complemented cells. Immunoblot analysis of potato (Solanum tuberosum) mitochondria demonstrated that PSD is located in mitochondrial membranes, and mRNA analysis in Arabidopsis showed that the mitochondrial PSD gene is expressed at low levels throughout the plant. An Arabidopsis knockup mutant grew normally but had 6- to 13-fold more mitochondrial PSD mRNA and 9-fold more mitochondrial PSD activity. Total membrane PSD activity was, however, unchanged in the mutant, showing mitochondrial activity to be a minor part of the total. These results establish that plants can synthesize Etn moieties via a phospholipid pathway and have both mitochondrial and extramitochondrial PSDs. They also indicate that mitochondrial PSD is an important housekeeping enzyme whose expression is strongly regulated at the transcriptional level.
The Tn Antigen-Specific Lectin from Ground Ivy Is an Insecticidal Protein with an Unusual Physiology
Authors: Wang Weifang , Hause Bettina , Peumans Willy J. , Smagghe Guy , Mackie Anne , Fraser Robin , Van Damme Els J.M. 
Published: July 2003

Abstract
Leaves of ground ivy (Glechoma hederacea) contain a lectin (called Gleheda) that is structurally and evolutionary related to the classical legume lectins. Screening of a population of wild plants revealed that Gleheda accounts for more than one-third of the total leaf protein in some clones, whereas it cannot be detected in other clones growing in the same environment. Gleheda is predominantly expressed in the leaves where it accumulates during early leaf maturation. The lectin is not uniformly distributed over the leaves but exhibits a unique localization pattern characterized by an almost exclusive confinement to a single layer of palisade parenchyma cells. Insect feeding trials demonstrated that Gleheda is a potent insecticidal protein for larvae of the Colorado potato beetle (Leptinotarsa decemlineata). Because Gleheda is not cytotoxic, it is suggested that the insecticidal activity is linked to the carbohydrate-binding specificity of the lectin, which as could be demonstrated by agglutination assays with different types of polyagglutinable human erythrocytes is specifically directed against the Tn antigen structure (N-acetylgalactosamine O-linked to serine or threonine residues of proteins).
Deviant Talk in Adolescent Friendships: A Step Toward Measuring a Pathogenic Attractor Process
Authors: Granic Isabela , Dishion Thomas J. 
Published: July 2003

AbstractDeviant talk in adolescent friendships has been previously found to predict escalations in substance use, delinquency, and violence. The current paper extends past work on deviant talk by examining its dynamic, self‐organizing properties. From the direct observations of peer interactions, a simple measure was developed that indicated whether, as an interaction unfolded, deviant talk bouts became longer in duration (indicating an attractor state). Participants included 102 high‐risk adolescents and their friends. A time‐series of the duration of each successive deviant talk bout over the course of an interaction was created for all dyads. Slope values were derived from the time‐series and used as an index of attractor strength. As hypothesized, the attractor index predicted serious authority conflict (arrests, school expulsion) and drug abuse three years later, after controlling for prior problem behavior, family coercion and deviant peer associations. The findings suggest that the process by which adolescents become increasingly absorbed in deviant talk is an important underlying mechanism in the development of serious antisocial behavior.
Mother–Son Positive Synchrony in Middle Childhood: Relation to Antisocial Behavior
Authors: Criss Michael M. , Shaw Daniel S. , Ingoldsby Erin M. 
Published: July 2003

Abstract The aim of the present investigation was to examine mother–son positive synchrony and its link to child and best friend antisocial behavior in middle childhood. Data were collected from 122 families with 10‐year‐old children during home assessments. Positive synchrony was rated during a parent–child discussion task. Data were also gathered on parent–child openness and conflict, harsh discipline, parental monitoring, and the child's social information processing. Four domains of child adjustment were assessed: antisocial behavior (ages 8 and 10), best friend antisocial behavior (ages 8 and 10), social skills (age 10), and anxiety/depression (age 10). The results indicated that observed positive synchrony was related significantly to measures tapping parenting, parent–child conflict, and child social information processing, as well as to youth and best friend antisocial behavior. The associations between synchrony and antisocial behavior remained significant after controlling for prior youth adjustment and other child and parenting factors. Developmental implications and suggestions for future research are discussed.
Change in Parents’ Monitoring Knowledge: Links with Parenting, Relationship Quality, Adolescent Beliefs, and Antisocial Behavior
Authors: Laird Robert D. , Pettit Gregory S. , Dodge Kenneth A. , Bates John E. 
Published: July 2003

Abstract A longitudinal prospective design was used to examine antisocial behavior, two aspects of the parent–child relationship, inept parenting, and adolescents’ beliefs in the appropriateness of monitoring as predictors of parents’ monitoring and change in monitoring during the high school years. A total of 426 adolescents provided reports of their parents’ monitoring knowledge during four yearly assessments beginning the summer before entering grade 9. Greater concurrent levels of monitoring knowledge were associated with less antisocial behavior, more parent‐reported relationship enjoy‐ment, adolescents and parents spending more time together, and adolescents reporting stronger beliefs in the appropriateness of parental monitoring. Weaker knowledge beliefs predicted increases in monitoring knowledge over time. More antisocial behavior problems were linked to lower levels of knowledge through less enjoyable parent–adolescent relationships, parents and adolescents spending less time together, and adolescents reporting weaker monitoring beliefs. Discussion focuses on processes linking antisocial behavior problems with low levels of monitoring knowledge.
Boys’ Maladaptive Social Information Processing, Family Emotional Climate, and Pathways to Early Conduct Problems
Authors: Schultz David , Shaw Daniel S. 
Published: July 2003

Abstract Maladaptive social information processing characterizes many children with behavioral adjustment difficulties. Yet, we know little about the antecedents of maladaptive social information processing. The present study examined the relations between early family risk and later maladaptive social information processing and conduct problems in a sample of 178 economically disadvantaged boys. Early childhood assessments of socioeconomic disadvantage (low SES, single parent status, low maternal education) and maternal depression predicted boys’ maladaptive response generation and conduct problems at age 10, accounting for 6% and 14% of the variances, respectively, but not hostile attribution bias. Maladaptive response generation also fit a model of significant partial mediation of the effects of socioeconomic disadvantage on conduct problems. Results suggest the importance of the family's emotional climate during children's early development for their later adjustment.
A First Assessment of Some Measures of Core Inflation for the Euro Area
Authors: Vega Juan-Luis , Wynne Mark A. 
Published: July 2003

AbstractCore inflation plays an important role in the deliberations of monetary policy-makers. In this paper we evaluate a number of measures of core inflation constructed using euro-area data. In addition to the traditional exclusion-type core measures, we examine two newer ones, documenting their properties and evaluating their performance in terms of their ability to track underlying or trend inflation in real time. We focus on core measures derived from the Harmonized Index of Consumer Prices (HICP) as the European Central Bank has chosen to define its mandate for price stability in terms of this index, and because this is the only index of consumer prices that is compiled in a comparable manner across all members of the European Union. We document significant excess kurtosis in the cross-section distribution of price changes in the euro area, and show that several categories of prices are more volatile than those typically excluded from traditional measures of core inflation. Contrary to what one might expect, traditional measures of core inflation are not significantly less volatile than headline measures.We document the superior performance of alternative measures of core inflation in tracking trend inflation on average, but show that none of the various measures of core gave significant advance warning of the pickup in trend inflation at the beginning of 1999.
Measuring Monetary Policy in Germany: A Structural Vector Error Correction Approach
Authors: Brüggemann Imke 
Published: July 2003

AbstractA structural vector error correction (SVEC) model is used to investigate several monetary policy issues. While being data-oriented the SVEC framework allows structural modeling of the short-run and long-run properties of the data. The statistical model is estimated with monthly German data for 1975-98 where a structural break is detected in 1984. After splitting the sample, three stable long-run relations are found in each subsample which can be interpreted in terms of a moneydemand equation, a policy rule and a relation for real output, respectively. Since the cointegration restrictions imply a particular shape of the long-run covariance matrix this information can be used to distinguish between permanent and transitory innovations in the estimated system. Additional restrictions are introduced to identify a monetary policy shock.
Monetary Policy, Agency Costs and Output Dynamics
Authors: Linnemann Ludger , Schabert Andreas 
Published: July 2003

Abstract
This paper examines the role of financial market imperfections for output reactions to nominal interest rate shocks. Empirical evidence shows a humpshaped impulse response function of output and suggests that credit supply co-moves with output. A monetary business cycle model with staggered price setting is presented where the firms’ outlays for capital and labor must be covered by the sum of net worth of entrepreneurs and loans in the form of debt contracts. These properties are shown to generate a hump-shaped impulse response of output, which takes on the smooth and persistent appearance of the empirical output response when nominal wages are set in a staggered way, too.
Monetary Policy Shocks and Heterogeneous Finance Decisions: A Model of Hidden Effort Choice and Financial Intermediation
Authors: Dietrich Diemo 
Published: July 2003

AbstractThe paper investigates how monetary policy shocks influence the composition of firms’ external finance given that firms are heterogeneous. Heterogeneity stems from differences in the availability of internal funds and in the monitoring costs associated with bank finance. These costs are determined by the intensity of the lending relationship. By using a delegated monitoring approach it is found that bank loans serve as a substitute for internal funds if the lending relationship is sufficiently close. Moreover, banks with strong credit ties to their customers are not only able to protect borrowers from liquidity constraints following a monetary tightening but are even able to extend their business lending.
Competition Necessarily Tends to Produce Excess: The Experience of Free Banking in Switzerland
Authors: Neldner Manfred 
Published: July 2003

Abstract
According to McCulloch, Longfield and Loyd, a free banking system is always prone to overissues of bank notes. Their view is supported by the free banking era in Switzerland (1826-1907), where, due to competitive pressures within the banking community and the absence of note-brand loyalty on the part of the general public, overissues (causing a rise in the foreign exchange rates above the upper gold and silver points) finally became permanent. Free competition, therefore, had to give way to collusive action and, in 1907 (with the open consent of the issuing banks), to the establishment of the Swiss National Bank.
Vaccinations for Adult Solid-Organ Transplant Recipients: Current Recommendations and Protocols
Authors: Duchini Andrea , Goss John A. , Karpen Saul , Pockros Paul J. 
Published: July 2003

SUMMARY
Recipients of solid-organ transplantation are at risk of severe infections due to their life-long immunosuppression. Despite emerging evidence that vaccinations are safe and effective among immunosuppressed patients, most vaccines are still underutilized in these patients. The efficacy, safety, and protocols of several vaccines in this patient population are poorly understood. Timing of vaccination appears to be critical because response to vaccinations is decreased in patients with end-stage organ disease and in the first 6 months after transplantation. For these reasons, the primary immunizations should be given before transplantation, as early as possible during the course of disease. Vaccination strategy should include vaccination of household contacts and health care workers at transplant centers unless contraindicated. No conclusive data are available on the use of immunoadjuvants and screening for protective titers. Most vaccines appear to be safe in solid-organ transplantation recipients, but live vaccines should be avoided until further studies are available. The risk of rejection appears minimal. Recommended vaccines include pneumovax, hepatitis A and B, influenza, and tetanus-diphtheria. We outline specific protocols and recommendations in this particular patient population. Specific contraindications exist for other vaccines, such as yellow fever, oral polio vaccine, bacillus Calmette-Guerin, and vaccinia. We conclude that solid-organ recipients will benefit from consistent immunization practices. Further studies are recommended to improve established protocols in this patient population.