bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parenting through place‐of‐care disruptions: A qualitative study of parents' experiences of neonatal care
Authors: Cupit Caroline , Paton Alexis , Boyle Elaine , Pillay Thillagavathie , Anderson Josie , Armstrong Natalie ,   the OPTI‐PREM team
Published: December 2023

AbstractIntroductionNeonatal care is complex, involving multiple people and technologies within a community of care. When preterm babies are cared for far from home and/or transferred between units, the whole community of care (and particularly parent participation) is disrupted. Although previous studies have captured subjective experiences of parents, there has been little research exploring the material practices undertaken by parents as a consequence of place‐of‐care decisions, or the social organisation of those practices.MethodsAs part of a wider study exploring optimal place‐of‐care, semistructured interviews were conducted between July 2018 and October 2019 with 48 parents (36 families) with one or more preterm babies (born at 27–31 weeks gestation) cared for in a neonatal unit in the last 12 months.FindingsWe highlight parents' labour‐intensive and stressful work to: (1) parent in the neonatal care community (an oversight role that goes beyond contemporary notions of ‘involvement’); (2) create continuity amid place‐of‐care disruptions; and (3) adapt to the managerial logics of neonatal care settings. Our analysis focuses on the work generated by managerial systems that organise place‐of‐care decision‐making and other efficiency‐focused practices. Parents are absorbed into negotiating institutional systems and diverted from routine parenting activities.ConclusionThose involved in the organisation and management of neonatal care should take account of how managerial systems impact parents' workload, ability to participate in their baby's community of care and, ultimately, on the wellbeing and development of babies and their families.Patient or Public ContributionThe OPTI‐PREM study embedded parents' experiences of neonatal care into the research, through a discrete workstream that employed qualitative methodology to capture parents' experiences—as reported in this paper. The OPTI‐PREM project was also supported by a Bliss volunteer parent panel, which was involved in designing and overseeing the research. Bliss ‘champion[s] the right for every baby born premature or sick to receive the best care by supporting families, campaigning for change and supporting professionals and enabling life‐changing research’ (https://www.bliss.org.uk/about-us/about-bliss). A representative of Bliss is a co‐author of this manuscript, and a parent representative (named in the Acknowledgements) provided feedback during its preparation.
‘Keeping it real’: A qualitative exploration of preferences of people with lived experience for participation and active involvement in mental health research in Australia
Authors: Dray Julia , Palmer Victoria J. , Banfield Michelle 
Published: December 2023

AbstractBackgroundHistorically, researchers have been apt at conducting research on, rather than with, the people who are the focus of their efforts. Such approaches often fail to effectively support and benefit the populations they are intended to. This study aimed to explore the preferences of people with lived experience for engagement with research either as research participants within studies, or through active involvement in mental health research.MethodsData for this paper were collected in three separate lived experience agenda‐setting studies conducted over a 9‐year period from 2013 to 2022; two group discussions and an open‐ended online survey. Data were combined and thematic analysis undertaken.ResultsParticipants described the inclusion of lived experience as a critical ingredient and the highest level of knowledge and expertise in mental health research that should lead to knowledge generation and research agendas. Participants discussed the importance and value of research that enables sharing experiences and stories, expressed a need for flexibility in research methods for choice and agency, and support for greater active involvement of people with lived experience across all stages of research. Participants also spoke to the need for perspective and knowledge generated from people with lived experience to have equal power in research, making space for lived experience voices across multiple aspects of research, and greater respect and recognition of the value of lived experience.ConclusionLived experience in mental health research is coming of age, but dedicated, cocreated development is needed to get it right. People with lived experience increasingly understand the value their experiential knowledge brings to the mental health research effort, and describe a wide range of ways that researchers can support them to be research participants, and to get actively involved. Power‐sharing, respect and recognition of lived experience as central to effective mental health research are the keys to ‘keeping it real’.Patient or Public ContributionPeople with lived experience of mental health problems or distress either personally, and/or as carers, family and kinship group members, were involved in the coideation and codesign of this research. All authors identify as people with lived experience.
Using ohmic heating to shorten the tempering time for frozen fish
Authors: Chysirichote Teerin , Phumchom Nahathai , Chintapum Sappayakorn , Klinthong Worasuang , Ohtaguchi Kazuhisa , Yoovidhya Tipaporn 
Published: December 2023

SummaryThawing is necessary to restore the freshness of fish before cooking because fish is often frozen to retain its freshness. Frequently, fish is partially thawed (or ‘tempered’) as it is easier to handle in this form than fully thawed fish. However, the conventional tempering techniques – immersion in water and still air tempering – take a long time and alter the fish's characteristics. Hence, the aim of this study was to use the heat generated inside the frozen fish, or ohmic heating, to alter the tempering process. Compared with conventional tempering operations, in this study, three voltage levels (20, 30 and 40 V/cm) were used for ohmic tempering. The fish quality and yield findings revealed that 30‐V/cm ohmic heating was more appropriate, even though ohmic heating at 40 V/cm provided the shortest tempering time. The main operating expense for ohmic tempering was the power cost of USD 58.5/100 kg, whereas the major operating expense for water immersion was the cost of water supply and wastewater treatment, which was around USD 108/100 kg. By using ohmic heating instead of still air and water immersion to temper the frozen fish, not only was less time required while retaining the freshness of the fish but the cost of tempering was also reduced.
Arthrospira platensis as future food: a review on functional ingredients, bioactivities and application in the food industry
Authors: Chang Mengwei , Liu Kunlun 
Published: December 2023

SummaryThe coronavirus disease 2019 (COVID‐2019) pandemic and other factors have significantly increased the number of people suffering from food shortages worldwide in 2020. As many as 720–811 million people, which is nearly 10% of the global population, are facing hunger. Thus, the need for substances that fulfil human nutritional requirements is growing to address this issue. Arthrospira platensis (A. platensis), commonly or commercially called spirulina, is an ideal product that contains various functional ingredients. Given them, various health benefits of A. platensis are proven gradually, including anti‐oxidant, immune regulation, anti‐inflammation, hypoglycaemia, anti‐bacterial and anti‐radiation properties. In recent years, functional ingredients of A. platensis are showing the potential to be functional foods. This paper gives an overview on the functional ingredients and the resulting health benefits of A. platensis summarises the research progress of A. platensis as functional foods in recent years and looks forward to its application development prospects.
MicroRNA‐based therapies: Revolutionizing the treatment of acute myeloid leukemia
Authors: Chandraprabha Vineetha Radhakrishnan , Anitha Geetha Raj John , Devipriya Padmakumar , Sreelatha Mahitha Mohanan , Hariharan Sreedharan 
Published: December 2023

AbstractMicroRNAs (miRNAs) are small noncoding epigenetic regulators that exert critical significance by influencing target mRNAs and governing gene expression patterns and cellular signaling pathways. miRNAs play a pivotal role in a wide array of biological processes, including cell differentiation, proliferation, and survival. Numerous miRNAs contribute to tumorigenesis and cancer progression by promoting tumor growth, angiogenesis, invasion, and immune evasion, while others exert tumor suppressive effects. From a clinical perspective, it has been demonstrated that numerous miRNAs are related to the prognosis in acute myeloid leukemia (AML) patients. They hold the potential to be utilized as biomarkers, aiding in improved treatment decision‐making. Moreover, a number of preclinical investigations have offered compelling evidence to create novel treatment approaches that target miRNAs in AML. This review highlights the clinical significance of miRNAs in the diagnosis, prognosis, and treatment response of adult patients with AML.
Pedigree diversity and implications for genetic selection of Katahdin sheep
Authors: Nilson Sara M. , Burke Joan M. , Murdoch Brenda M. , Morgan James L. M. , Lewis Ronald M. 
Published: December 2023

AbstractThe Katahdin hair breed gained popularity in the United States as low input and prolific, with a propensity to exhibit parasite resistance. With the introduction of genomically enhanced estimated breeding values (GEBV) to the Katahdin genetic evaluation, defining the diversity present in the breed is pertinent. Utilizing pedigree records (n = 92,030) from 1984 to 2019 from the National Sheep Improvement Program, our objectives were to (i) estimate the completeness and quality of the pedigree, (ii) calculate diversity statistics for the whole pedigree and relevant reference subpopulations and (iii) assess the impact of current diversity on genomic selection. Reference 1 was Katahdins born from 2017 to 2019 (n = 23,494), while reference 2 was a subset with at least three generations of Katahdin ancestry (n = 9327). The completeness of the whole pedigree, and the pedigrees of reference 1 and reference 2, were above 50% through the fourth, fifth and seventh generation of ancestors, respectively. Effective population size (Ne) averaged 111 animals with a range from 42.2 to 451.0. The average generation interval was 2.9 years for the whole pedigree and reference 1, and 2.8 years for reference 2. The mean individual inbreeding and average relatedness coefficients were 1.62% and 0.91%, 1.74% and 0.90% and 2.94% and 1.46% for the whole pedigree, reference 1, and reference 2, respectively. There were over 300 effective founders in the whole pedigree and reference 1, with 169 in reference 2. Effective number of ancestors were over 150 for the whole pedigree and reference 1, while there were 67 for reference 2. Prediction accuracies increased as the reference population grew from 1k to 7.5k and plateaued at 15k animals. Given the large number of founders and ancestors contributing to the base genetic variation in the breed, the Ne is sufficient to maintain diversity while achieving progress with selection. Stable low rates of inbreeding and relatedness suggest that incorporating genetic conservation in breeding decisions is currently not of high priority. Current Ne suggests that with limited genotyping, high levels of accuracy for genomic prediction can be achieved. However, intense selection on GEBV may cause loss of genetic diversity long term.
Inverted U‐shaped associations between serum uric acid and fasting ‐ plasma glucose level in non‐diabetic, pre‐diabetic, and diabetic adults: A population‐based study in China
Authors: Shi Hongjuan , Liu Yining , Yang Danyu , Liang Peifeng , Chen Chen , Luan Hong , Shi Chao 
Published: December 2023

ABSTRACTObjectiveThis study was designed to examine the correlation between serum uric acid (SUA) and fasting plasma glucose (FPG) levels across non‐diabetic, pre‐diabetic, and diabetic adults from Northwest China.Materials and MethodsThis study utilized data from a cross‐sectional survey conducted in Ningxia Hui Autonomous Region, which investigated the prevalence and risk factors of cardiovascular disease. All subjects underwent tests for SUA and FPG levels. Generalized additive models and two‐piecewise linear regression models were applied to explore the relationships between SUA and FPG level. The triglyceride–glucose (TyG) index was examined as a measure of insulin resistance, with an analysis of its mediating effects on the association between SUA and FPG level.ResultsA total of 10,217 individuals aged 18 and over were included. Generalized additive models verified the inverted U‐shaped association between SUA and FPG levels, and the inflection points of FPG levels in the curves were 6.5 mmol/L in males and 8.8 mmol/L in females. The TyG index is an intermediate variable in the relationship between SUA levels and elevated FPG levels, with mediating effects of 12.82% (P < 0.001) for males and 34.02% (P < 0.001) for females.ConclusionsAn inverted U‐shaped association between FPG and SUA levels was observed in both genders. The threshold of FPG level was lower in males than in females. The relationship between these variables seems to be partially mediated by serum insulin levels.
Designing salt stress‐resilient crops: Current progress and future challenges
Authors: Liang Xiaoyan , Li Jianfang , Yang Yongqing , Jiang Caifu , Guo Yan 
Published: December 2023

ABSTRACTExcess soil salinity affects large regions of land and is a major hindrance to crop production worldwide. Therefore, understanding the molecular mechanisms of plant salt tolerance has scientific importance and practical significance. In recent decades, studies have characterized hundreds of genes associated with plant responses to salt stress in different plant species. These studies have substantially advanced our molecular and genetic understanding of salt tolerance in plants and have introduced an era of molecular design breeding of salt‐tolerant crops. This review summarizes our current knowledge of plant salt tolerance, emphasizing advances in elucidating the molecular mechanisms of osmotic stress tolerance, salt‐ion transport and compartmentalization, oxidative stress tolerance, alkaline stress tolerance, and the trade‐off between growth and salt tolerance. We also examine recent advances in understanding natural variation in the salt tolerance of crops and discuss possible strategies and challenges for designing salt stress‐resilient crops. We focus on the model plant Arabidopsis (Arabidopsis thaliana) and the four most‐studied crops: rice (Oryza sativa), wheat (Triticum aestivum), maize (Zea mays), and soybean (Glycine max).
The gut microbiota composition and metabolites are different in women with hypertensive disorders of pregnancy and normotension: A pilot study
Authors: Wu Zhouyi , Ge Mengdi , Liu Jinsu , Chen Xiaoqing , Cai Zhiqiang , Huang Huan 
Published: December 2023

AbstractIntroductionHypertensive disorders of pregnancy (HDP) are one of the main causes of perinatal morbidity. Gut microbiota influences host inflammatory pathways, glucose, and lipid metabolism. However, there is a lack of studies available on gut microbiota in HDP.ObjectivesWe investigate the mechanistic and pathogenic role of microbiota in the development of HDP, and want to treat HDP with gut microbiota.MethodsWe performed a case–control study to compare fecal samples of HDP and normotensive pregnant women by 16S ribosomal RNA sequencing. Fecal samples, collected from pregnant women, were divided into groups P and C (pregnant women with HDP and normotension, respectively). There were six pregnant women in group P and nine pregnant women in group C. Age of pregnant women is from 18 to 40 years and gestational age is from 27 to 40 weeks. DNA was extracted from fecal samples; a gene library was constructed and analyzed using bioinformatics. Finally, we determined the changes in the microbiome by alpha diversity, beta diversity, classification abundance, and taxonomic composition analyses.ResultsEscherichia (10.48% in group P and 0.61% in group C) was the dominant bacterium in HDP patients by classification abundance analysis, which can lead to the development of preeclampsia through inflammatory response. We found that pregnant women with HDP had higher abundance of Rothia (p = 0.04984), Actinomyces (p = 0.02040), and Enterococcus (p = 0.04974) and lower abundance of Coprococcus (p = 0.04955) than pregnant women with normotension for the first time by taxonomic composition analysis. Based on the Kyoto Encyclopedia of Genes and Genomes database analysis, physiological and biochemical functions of HDP patients were significantly weakened, especially in energy metabolism.ConclusionsWe found the effect of changes in gut microbiota on the development of HDP. In comparison with group C, group P contained more harmful bacteria and less beneficial bacteria, which are associated with HDP. Our research further provides a basis for a clinical application for HDP treatment using antibiotics and probiotic supplementation.
Postpartum pain and the risk of postpartum depression: A meta‐analysis of observational studies
Authors: Lu Limei , Shen Ya 
Published: December 2023

AbstractObjective: This meta‐analysis of observational studies aimed to derive a more precise estimation of the relationship between postpartum pain and postpartum depression (PPD).Methods: A systematic literature search was completed in the following databases from inception to September 26, 2022: PubMed, Embase, and Web of Science. Quality evaluation of each study was achieved through Newcastle‐Ottawa scale (NOS) assessment. Heterogeneity across studies was evaluated by Cochran's Q test and I2 test. Pooled estimates of odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were analyzed using fixed‐effects model or random‐effects model, according to heterogeneity. Subgroup analysis, sensitivity analysis, and Egger's test were also performed.Results: From the identified 1884 articles, a total of 8 studies involving 3973 participants were included in the final meta‐analysis. Seven of the 8 studies were evaluated as high‐quality, with NOS scores ≥7. A significant heterogeneity was observed (I2 = 66.5%, p = 0.004) among eight studies. Therefore, the performed random‐effect model suggested a significant association between postpartum pain and PPD risk (OR 1.29, 95% CI 1.10–1.52, p = 0.002). However, the subgroup analyses did not define the source of heterogeneity. Moreover, the sensitivity analysis showed the stability of the pooled results, but the significant publication bias was identified (p = 0.009). The trim and fill method was performed and resulted in an OR of 1.14 (95% CI 0.95–1.37, p = 0.162).Conclusions: This meta‐analysis found a potential association between postpartum pain and PPD. Further researches are needed to provide more robust evidences.
Purpose and goal pursuit as a self‐sustaining system: Evidence of daily within‐person reciprocity among adolescents in self‐driven learning
Authors: Ratner Kaylin , Gladstone Jessica R. , Zhu Gaoxia , Li Qingyi , Estevez Melody , Burrow Anthony L. 
Published: December 2023

AbstractObjectiveDespite long‐standing assumptions that a sense of purpose in life and goal pursuit are mutually supportive, empirical evidence of their reciprocity remains deficient. In the context of a unique out‐of‐school time program that empowers youth to pursue passions through self‐driven learning, we examined whether purpose and one aspect of goal pursuit—perceptions of goal progress—work together to sustain themselves and each other over time.MethodAdolescents (N = 321) completed daily surveys throughout program enrollment (Menrollment = 69.09 days). Through dynamic structural equation modeling, we derived within‐person patterns of day‐to‐day prediction as well as individual differences in these patterns.ResultsWe found purpose and perceived goal progress exhibited significant daily inertia (i.e., autoregressive prediction) and reciprocity (i.e., cross‐lagged prediction) at the within‐person level. We also found initial evidence suggesting (a) tighter reciprocity was related to greater perceived goal progress overall and (b) people with greater purpose inertia may rely less on making goal progress to sustain momentum.ConclusionsWith evidence of daily purpose‐progress reciprocity, the field can look forward to replicating this work in other contexts, diving deeper into interesting patterns of within‐person dynamics, and developing interventions to support youth striving.
Targeted HBx gene editing by CRISPR/Cas9 system effectively reduces epithelial to mesenchymal transition and HBV replication in hepatoma cells
Authors: Rawal Preety , Tripathi Dinesh Mani , Hemati Hamed , Kumar Jitendra , Tyagi Purnima , Sarin Shiv Kumar , Nain Vikrant , Kaur Savneet 
Published: December 2023

AbstractBackground and AimsHepatitis B virus X protein (HBx) play a key role in pathogenesis of HBV‐induced hepatocellular carcinoma (HCC) by promoting epithelial to mesenchymal transition (EMT). In this study, we hypothesized that inhibition of HBx is an effective strategy to combat HCC.Methodology and ResultsWe designed and synthesized novel HBx gene specific single guide RNA (sgRNA) with CRISPR/Cas9 system and studied its in vitro effects on tumour properties of HepG2‐2.15. Full length HBx gene was excised using HBx‐CRISPR that resulted in significant knockdown of HBx expression in hepatoma cells. HBx‐CRISPR also decreased levels of HBsAg and HBV cccDNA expression. A decreased expression of mesenchymal markers, proliferation and tumorigenic properties was observed in HBx‐CRISPR treated cells as compared to controls in both two‐ and three‐ dimensional (2D and 3D) tumour models. Transcriptomics data showed that out of 1159 differentially expressed genes in HBx‐CRISPR transfected cells as compared to controls, 70 genes were upregulated while 1089 genes associated with cell proliferation and EMT pathways were downregulated.ConclusionThus, targeting of HBx by CRISPR/Cas9 gene editing system reduces covalently closed circular DNA (cccDNA) levels, HBsAg production and mesenchymal characteristics of HBV‐HCC cells. We envision inhibition of HBx by CRISPR as a novel therapeutic approach for HBV‐induced HCC.