bluuebunny/crossref_metadata_2025_split huggingface dataset:
A biomarker panel of secondary hypertension is simultaneously quantified by coupling of magnetic solid‐phase extraction and liquid chromatography–tandem mass spectrometry
Authors: Gao Xi , Li Xiaoyong , Chen Lingyun , Chen Shuyan , Hou Guixue , Lin Liang , Wang Qunjie , Qu Jiuxin , Liu Siqi 
Published: January 2024

RationaleSecondary hypertension is often caused by activation of complex multi‐organ endocrine systems, while renin activity indicated by angiotensins (Angs), aldosterone (ALD) and cortisol (COR) in such systems are generally accepted as its diagnostic markers. As antibody‐based methods cannot offer comparable quantification for these biomarkers, a liquid chromatography (LC)–tandem mass spectrometry (MS/MS)‐based approach was developed to quantify them simultaneously and accurately.MethodsFive different beads for magnetic solid‐phase extraction (MSPE) were evaluated towards their enrichment efficiency for these biomarkers. An LC system with optimized elution gradient and a triple‐quadrupole MS with tuned parameters were coupled to quantitatively monitor the extracted analytes. The method performance was further examined such as linearity, precision, stability, recovery rate and matrix effect. Based on the developed method, the abundance of Ang II, ALD and COR in plasma was measured and the quantification was compared with that derived from commercial ELISA kits.ResultsAs compared with other MSPEs, Angs, ALD and COR were highly enriched by the HLB magnetic beads with satisfactory recoveries. These analytes were simultaneously quantified by LC/MS/MS and all the method parameters for quantification were well matched with the requirements of clinical testing. Comparison of the quantitative results derived from ELISA and LC/MS/MS exhibited that the two methods offered basically comparable values with Pearson r values at 0.896, 0.895 and 0.835, respectively. The stability test for plasma Angs at room temperature indicated that the abundance of Ang II was relatively stable within 3 h, whereas that of Ang I and Ang 1–7 was time‐dependently changed.ConclusionsCoupling of HLB beads and LC/MS/MS thus enables simultaneous quantification of a set of biomarkers related to secondary hypertension.
Drug‐induced lung injury in a patient treated with prior atezolizumab and subsequent sotorasib: A case report
Authors: Takahashi Yuta , Tachi Hiroaki , Watanabe Ryo , Shimizu Kei , Yamamoto Yusuke 
Published: January 2024

AbstractWe report a case of drug‐induced lung injury treated with prior atezolizumab and subsequent sotorasib. The patient was a 62‐year‐old woman with lung adenocarcinoma harbouring a KRAS G12C mutation that was resistant to chemotherapy, including immune checkpoint inhibitors. Cough and dyspnea appeared on day 80 after sotorasib was administered as second‐line therapy, and chest computed tomography revealed ground glass opacities in all lung lobes. Bronchoalveolar lavage fluid showed an increased total cell count with lymphocyte predominance. The patient was considered to have lung injury caused by prior atezolizumab or sotorasib administration. Withdrawal of sotorasib did not improve symptoms and shadows in both lungs. We administered moderate‐dose prednisolone and the lung disorder quickly resolved. Prednisolone tapering was completed in 2 months, followed by several months without relapse. Definitive identification of the responsible drug for the drug‐induced lung injury proved challenging in the setting of exposure to multiple potential inciting agents. There is a need for high levels of clinical suspicion for timely evaluation and management.
Immunogenicity, clinical efficacy and safety of additional second COVID‐19 booster vaccines against Omicron and its subvariants: A systematic review
Authors: Chenchula Santenna , Chandra Madhu Bhargavi , Adusumilli Madhu Babu , Ghanta Sai Nikhila , Bommasani Anusha , Kuttiappan Anitha , Padmavathi R. , Amerneni Krishna Chaitanya , Chikatipalli Radhika , Ghanta Mohan Krishna , Reddy Samarra Simha , Mythili Bai K. , Prakash Satya , Jogender G. , Chavan Madhavrao , Balakrishnan S. 
Published: January 2024

AbstractThe Omicron variant of severe acute respiratory syndrome coronavirus 2 is a new variant of concern (VOC) and an emerging subvariant that exhibits heightened infectivity, transmissibility, and immune evasion, escalating the incidence of moderate to severe coronavirus disease 2019 (COVID‐19). It resists monoclonal antibodies and diminishes vaccine efficacy. Notably, new sublineages have outpaced earlier predominant sublineages. Although the primary vaccination series and initial boosters were robust against previous VOCs, their efficacy waned against Omicron and its subvariants. In this systematic review, we assessed real‐world evidence on the immunogenicity, clinical efficacy, and safety of a second booster or fourth COVID‐19 vaccine dose against the Omicron VOC and its subvariants. A comprehensive literature search was conducted in Medline/PubMed, Google Scholar, bioRxiv, and medRxiv, and relevant studies published between 2022 and 30 May 2023 were reviewed. We found a total of 40 relevant articles focusing on a second booster dose for COVID‐19, including clinical trials and observational studies, involving 3,972,856 patients. The results consistently revealed that an additional second booster dose restored and prolonged waning immunity, activating both humoral and cellular responses against Omicron and its subvariants. A second booster treatment correlated with enduring protection against COVID‐19, notably preventing substantial symptomatic disease and mortality associated with severe Omicron infection. Both monovalent messenger RNA (mRNA) and nonmRNA vaccines demonstrated similar efficacy and safety, with bivalent mRNA vaccines exhibiting broader protection against emerging subvariants of Omicron. The safety profiles of second booster were favourable with only mild systemic and local symptoms reported in some recipients. In conclusion, this systematic review underscores the additional COVID‐19 vaccine boosters, particularly with bivalent or multivalent mRNA vaccines, for countering the highly infectious emerging subvariants of Omicron.
Effects of SNP marker density and training population size on prediction accuracy in alfalfa (Medicago sativa L.) genomic selection
Authors: Wang Hu , Bai Yuguang , Biligetu Bill 
Published: January 2024

AbstractEffects of individual single‐nucleotide polymorphism (SNP) markers and the size of “training” and “test” populations affect prediction accuracy in genomic selection (GS). This study evaluated 11 subsets of 4932 SNPs using six genetic additive methods to understand marker density in GS prediction in alfalfa (Medicago sativa L.). In the GS methods, the effect of “training” to “test” population size was also evaluated. Fourteen alfalfa populations sampled from long‐term grazing sites were genotyped using genotyping by sequencing for the identification of SNPs. These populations were also phenotyped for six agromorphological and three nutritive traits from 2018 to 2020. The accuracy of GS prediction improved across six GS methods when the ratio of “training” to “test” population size increased. However, the prediction accuracy of the six GS methods reduced to a range of −0.27 to 0.11 when random, uninformative SNPs were used. In this study, five Bayesian methods and ridge‐regression best linear unbiased prediction (rrBLUP) method had similar GS accuracies for “training” sets, but rrBLUP tended to outperform Bayesian methods in independent “test” sets when SNP subsets with high mean‐squared‐estimated‐marker effect were used. These findings can enhance the application of GS in alfalfa genetic improvement.
New physics searches using ProtoDUNE and the CERN SPS accelerator
Authors: Coloma Pilar , López-Pavón Jacobo , Molina-Bueno Laura , Urrea Salvador 
Published: January 2024

Abstract
The exquisite capabilities of liquid Argon Time Projection Chambers make them ideal to search for weakly interacting particles in Beyond the Standard Model scenarios. Given their location at CERN the ProtoDUNE detectors may be exposed to a flux of such particles, produced in the collisions of 400 GeV protons (extracted from the Super Proton Synchrotron accelerator) on a target. Here we point out the interesting possibilities that such a setup offers to search for both long-lived unstable particles (Heavy Neutral Leptons, axion-like particles, etc) and stable particles (e.g. light dark matter, or millicharged particles). Our results show that, under conservative assumptions regarding the expected luminosity, this setup has the potential to improve over present bounds for some of the scenarios considered. This could be done within a short timescale, using facilities that are already in place at CERN, and without interfering with the experimental program in the North Area at CERN.
Establishing a Rat Model of Pelvic Organ Prolapse with All Compartment Defects by Persistent Cervical Tension
Authors: Bai Siqi , Lu Chenxi , Kong Qingyu , Shen Zhuowei , Li Rui , Xiao Zhen 
Published: January 2024

Abstract
Introduction and Hypothesis
We hypothesized that applying cervical suction and persistent tension can develop a novel and efficient rat model of pelvic organ prolapse.

Methods
Fifteen rats underwent pilot testing to optimize the protocol. Sixteen rats were subjected to pelvic organ prolapse induction by cervical suction and constant traction, while five rats served as controls. The pelvic organ prolapse rats were assessed by a Rat Pelvic Organ Prolapse Quantification system at different time points, and their diet, urine, and stool were monitored for 21 days. The pelvic organ prolapse rats were also evaluated for urinary incontinence, urinary retention, leak point pressure, and vaginal histopathology at 21 days after operation.

Results
This rat model demonstrated pelvic floor prolapse in anatomic level, as well as physiological variations (urine incontinence, urinary retention) and pathological changes (collagen fracture, decreased collagen density).

Conclusions
This is the first establishment of the pelvic organ prolapse rat model with all compartment defects, which provides a valuable tool for elucidating pelvic organ prolapse mechanisms and evaluating potential interventions.
Inferior long-term outcomes after surgery for lumbar disc herniation in patients with prior lumbar spine surgery
Authors: Roiha Miika , Marjamaa Johan , Siironen Jari , Koski-Palkén Anniina 
Published: January 2024

Abstract

Background
Previous lumbar spine surgery is a frequent exclusion criterion for studies evaluating lumbar surgery outcomes. In real-life clinical settings, this patient population is important, as a notable proportion of patients evaluated for lumbar spine surgery have undergone prior lumbar surgery already previously. Knowledge about the long-term outcomes after microdiscectomy on patients with previous lumbar surgery and how they compare to those of first-time surgery is lacking.

Methods
The original patient cohort for screening included 615 consecutive patients who underwent surgery for lumbar disc herniation, with a median follow-up time of 18.1 years. Of these patients, 89 (19%) had undergone lumbar spine surgery prior to the index surgery. Propensity score matching (based on age, sex, and follow-up time) was utilized to match two patients without prior surgery with each patient with a previous surgery. The primary outcome measure was the need for further lumbar spine surgery during the follow-up period, and the secondary outcome measures consisted of present-time patient-reported outcome measures (Oswestry Disability Index, EuroQol-5D) and present-time ability to carry out employment.

Results
Patients who received previous lumbar surgeries had a higher need for further surgery (44% vs. 28%, p = 0.009) and had a shorter time to further surgery than the propensity score-matched cohort (mean Kaplan–Meier estimate, 15.7 years vs. 19.8 years, p = 0.008). Patients with prior surgery reported inferior Oswestry Disability Index scores (13.7 vs. 8.0, p = 0.036). and EQ-5D scores (0.77 vs. 0.86, p = 0.01). In addition, they had a higher frequency of receiving lumbar spine-related disability pensions than the other patients (12% vs. 1.9%, p = 0.01).

Conclusions
Patients with previous lumbar surgery had inferior long-term outcomes compared to patients without prior surgery. However, the vast majority of these patients improved quickly after the index surgery. Furthermore, the difference in the patients’ reported outcomes was small at the long-term follow-up, and they reported high satisfaction with the results of the study surgery. Hence, surgery for these patients should be considered if surgical indications are met, but special care needs must be accounted for when deliberating upon their indications for surgery.
Is there a difference in surgical accuracy following bimaxillary surgery between cleft and non-cleft patients?
Authors: Beek Dirk-Melle , Visser Dirk-Jan , Chen Yi-Hsuan , Baan Frank , Nienhuijs Marloes , Xi Tong 
Published: January 2024

Abstract
Objectives
To assess the surgical accuracy of 3D virtually planned orthognathic surgery among patients with and without cleft.

Materials and methods
This retrospective cohort study included cleft and non-cleft patients with class III malocclusion who underwent bimaxillary surgery. CBCT scans were acquired before and immediately after surgery. 3D virtual surgical planning (VSP) was performed using CBCT and digitalized dentition data. All orthognathic surgeries were performed by the same surgeons using interocclusal splints. The primary outcome variable was surgical accuracy, defined as the difference between the planned and surgically achieved maxillary movements, quantified in six degrees of freedom. Analysis of covariance was used to test for intergroup differences in surgical accuracy after correcting for differences in the magnitude of planned surgical maxillary movements.

Results
Twenty-eight cleft and 33 non-cleft patients were enrolled, with mean ages of 18.5 and 25.4 years, respectively (P=0.01). No significant gender difference was present between the groups (P=0.10). After adjustment for small differences in surgical movements, no significant differences in surgical accuracy were observed between cleft and non-cleft patients.

Conclusion
The present study demonstrates that high surgical accuracy in maxillary movements can be achieved in both cleft and non-cleft patients using VSP and interocclusal splints.

Clinical relevance
Orthognathic cases with cleft can be performed with 3D VSP to obtain a satisfactory surgical accuracy.
Psychological Reactance to Anti-Piracy Messages explained by Gender and Attitudes
Authors: Whitman Kate , Murad Zahra , Cox Joe 
Published: January 2024

AbstractDigital piracy is costly to creative economies across the world. Studies indicate that anti-piracy messages can cause people to pirate more rather than less, suggesting the presence of psychological reactance. A gender gap in piracy behavior and attitudes towards piracy has been reported in the literature. By contrast, gender differences in message reactance and the moderating impact of attitudes have not been explored. This paper uses evolutionary psychology as a theoretical framework to examine whether messages based on real-world anti-piracy campaigns cause reactance and whether this effect is explained by gender and pre-existing attitudes. An experiment compares one prosocial and two threatening messages against a control group to analyze changes in piracy intention from past behavior for digital TV/film. Results indicate that the prosocial message has no significant effect, whereas the threatening messages have significantly opposing effects on men and women. One threatening message influences women to reduce their piracy intentions by over 50% and men to increase it by 18%. We find that gender effects are moderated by pre-existing attitudes, as men and women who report the most favorable attitudes towards piracy tend to demonstrate the most polarized changes in piracy intentions. The practical implications of the results are that men and women process threatening messages differently, therefore behavioral change messages should be carefully targeted to each gender. Explicitly, threatening messages may be effective on women, but may have the reverse effect on men with strong favorable attitudes towards the target behavior.
$$\pi $$HyFlow: formalism, semantics, and applications
Authors: Barros Fernando 
Published: January 2024

AbstractSimulation models have been described using different perspectives, or worldviews. In the process interaction world view (PI), every entity is modeled by a sequence of actions describing its life cycle, offering a comprehensive model that groups the events involving each entity. In this paper we describe $$\pi $$
π
HyFlow, a formalism for representing hybrid models using a set of communicating processes. This set is dynamic, enabling processes to be created and destroyed at runtime. Processes are encapsulated into $$\pi $$
π
HyFlow base models and communicate through shared memory. $$\pi $$
π
HyFlow, however, can guarantee modularity by enforcing that models can only communicate by input and output interfaces. $$\pi $$
π
HyFlow extends current PI approaches by providing support for HyFlow concepts of sampling and dense (continuous) outputs, in addition to the more traditional event-based communication. Likewise HyFlow, $$\pi $$
π
HyFlow is a modeling & simulation formalism driven by expressiveness and performance analysis. We present $$\pi $$
π
HyFlow semantics, and several applications to illustrate $$\pi $$
π
HyFlow ability to describe a diversity of systems.
Beyond symmetry in generalized Petersen graphs
Authors: García-Marco Ignacio , Knauer Kolja 
Published: January 2024

AbstractA graph is a core or unretractive if all its endomorphisms are automorphisms. Well-known examples of cores include the Petersen graph and the graph of the dodecahedron—both generalized Petersen graphs. We characterize the generalized Petersen graphs that are cores. A simple characterization of endomorphism-transitive generalized Petersen graphs follows. This extends the characterization of vertex-transitive generalized Petersen graphs due to Frucht, Graver, and Watkins and solves a problem of Fan and Xie. Moreover, we study generalized Petersen graphs that are (underlying graphs of) Cayley graphs of monoids. We show that this is the case for the Petersen graph, answering a recent mathoverflow question, for the Desargues graphs, and for the Dodecahedron—answering a question of Knauer and Knauer. Moreover, we characterize the infinite family of generalized Petersen graphs that are Cayley graphs of a monoid with generating connection set of size two. This extends Nedela and Škoviera’s characterization of generalized Petersen graphs that are group Cayley graphs and complements results of Hao, Gao, and Luo.
Building Capacity for Research on Community Doula Care: A Stakeholder-Engaged Process in California
Authors: Marshall Cassondra J. , Nguyen Ashley , Arteaga Stephanie , Hubbard Erin , Armstead Marna , Peprah-Wilson Sayida , Britt Starr , McLemore Monica R. , Gomez Anu Manchikanti 
Published: January 2024

Abstract
Purpose
In an effort to address persistent inequities in maternal and infant health, policymakers and advocates have pushed to expand access to doula care. Several states, including California, now cover doula services through Medicaid. As coverage expands, research on the impact of doula care will likely increase. To develop best practices for research, it is critical to engage community doulas, clients, and other key stakeholders.

Description
Our overarching goal was to build capacity for future doula- and client-centered research on community doula care. First, we established a Steering Committee with members from seven relevant stakeholder groups: community doulas, former or potential doula clients, clinicians, payers, advocates, researchers, and public health professionals. Second, we conducted a needs assessment to identify and understand stakeholders’ needs and values for research on community doula care. Findings from the needs assessment informed our third step, conducting a research prioritization to develop a shared research agenda related to community doula care with the Steering Committee. We adapted the Research Prioritization by Affected Communities protocol to guide this process, which resulted in a final list of 21 priority research questions. Lastly, we offered a training to increase capacity among community doulas to engage in research on community doula care.

Assessment
Our findings provide direction for those interested in conducting research on doula care, as well as policymakers and funders.

Conclusion
The findings of our stakeholder-engaged process provide a roadmap that will lead to equity-oriented research centering clients, doulas, and their communities.
Administering selected subscales of patient-reported outcome questionnaires to reduce patient burden and increase relevance: a position statement on a modular approach
Authors: Serrano Daniel , Cella David , Husereau Don , King-Kallimanis Bellinda , Mendoza Tito , Salmonson Tomas , Stone Arthur , Zaleta Alexandra , Dhanda Devender , Moshyk Andriy , Liu Fei , Shields Alan L. , Taylor Fiona , Spite Sasha , Shaw James W. , Braverman Julia 
Published: January 2024

AbstractPatient-reported outcome (PRO) questionnaires considered in this paper contain multiple subscales, although not all subscales are equally relevant for administration in all target patient populations. A group of measurement experts, developers, license holders, and other scientific-, regulatory-, payer-, and patient-focused stakeholders participated in a panel to discuss the benefits and challenges of a modular approach, defined here as administering a subset of subscales out of a multi-scaled PRO measure. This paper supports the position that it is acceptable, and sometimes preferable, to take a modular approach when administering PRO questionnaires, provided that certain conditions have been met and a rigorous selection process performed. Based on the experiences and perspectives of all stakeholders, using a modular approach can reduce patient burden and increase the relevancy of the items administered, and thereby improve measurement precision and eliminate wasted data without sacrificing the scientific validity and utility of the instrument. The panelists agreed that implementing a modular approach is not expected to have a meaningful impact on item responses, subscale scores, variability, reliability, validity, and effect size estimates; however, collecting additional evidence for the impact of context may be desirable. It is also important to recognize that adequate rationale and evidence (e.g., of fit-for-purpose status and relevance to patients) and a robust consensus process that includes patient perspectives are required to inform selection of subscales, as in any other measurement circumstance, is expected. We believe that the considerations discussed within (content validity, administration context, and psychometric factors) are relevant across multiple therapeutic areas.