bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin supplementation effect on liver enzymes in patients with nonalcoholic fatty liver disease: a GRADE-assessed systematic review and dose–response meta-analysis of randomized controlled trials
Authors: Vajdi Mahdi , Hassanizadeh Shirin , Hassanizadeh Reza , Bagherniya Mohammad 
Published: December 2023

Abstract

Context
Clinical evidence from investigations of the effects of curcumin on liver enzymes in patients with nonalcoholic fatty liver disease (NAFLD) have led to inconsistent results.

Objective
The aim of this systematic review and meta-analysis was to investigate the overall effects of curcumin and curcumin plus piperine supplementation on liver enzymes such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) in patients with NAFLD.

Data Sources
The Scopus, Web of Science, PubMed, and Cochrane Library databases were searched from inception through July 2023, using search terms representing NAFLD and liver enzymes. Articles were screened independently by 2 researchers based on PICOS inclusion criteria.

Data Extraction
The following data were extracted: first author’s name, study location, year of publication, mean age, study duration, study design, participants’ sex, number of participants in each group, dose of curcumin supplementation, and ALT, ALP, and AST concentrations. Risk of bias was assessed using the Cochrane Collaboration’s modified risk-of-bias tool.

Data Analysis
Fixed- or random-effects meta-analysis was performed to estimate the effects of curcumin on liver enzymes, considering heterogeneity across studies. The I2 and Cochran’s Q tests were used to assess heterogeneity between studies.

Results
Overall, 15 randomized controlled trials comprising 905 participants were eligible for this meta-analysis. Curcumin supplementation significantly reduced ALT (weighted mean difference [WMD], –4.10, 95%CI, –7.16 to –1.04) and AST (WMD, –3.27; 95%CI, –5.16 to –1.39), but not ALP (WMD, –0.49; 95%CI, –1.79 to 0.82). Curcumin plus piperine supplementation had no significant effect on ALT (WMD, –3.79; 95%CI, –13.30 to 5.72), and AST (WMD, –1.1; 95%CI, –3.32 to 1.09).

Conclusions
Curcumin supplementation improved AST and ALT levels compared with the control group. However, better-designed randomized controlled trials with larger sample sizes and of higher quality are needed to assess the effects of curcumin on ALP.

Systematic Review Registration
PROSPERO registration no. CRD42023448231.
Mutational Signature Changes in Patients With Metastatic Squamous Cell Carcinoma of the Anal Canal
Authors: LaPelusa Michael , Cann Christopher , Ciombor Kristen K , Eng Cathy 
Published: December 2023

Abstract

Purpose
We examined the concordance of genetic mutations between pretreatment tumor tissue and posttreatment circulating tumor DNA (ctDNA) in patients with metastatic squamous cell carcinoma of the anal canal (SCCA) and assessed the impact of therapy on this concordance.

Methods
We analyzed next-generation sequencing reports from pretreatment tumor tissue and posttreatment ctDNA in 11 patients with metastatic SCCA treated at Vanderbilt University Medical Center between 2017 and 2021.

Results
Among the mutations identified in posttreatment ctDNA, 34.5% were also found in pretreatment tumor tissue, while 47.6% of pretreatment tumor tissue mutations were found in posttreatment ctDNA. Four patients had preservation of potentially actionable mutations in both pretreatment tissue and posttreatment ctDNA, while 7 patients had newly identified mutations in posttreatment ctDNA that were not present in pretreatment tumor tissue.

Conclusion
Patients with SCCA demonstrate a high degree of temporal mutational heterogeneity. This supports the hypothesis that ctDNA can serve as a real-time tracking mechanism for solid tumors’ molecular evolution in response to therapy. Our findings highlight the potential of ctDNA in identifying emerging actionable mutations, supplementing information from tissue-based genomic assessments. Further research, ideally with larger and multi-institutional cohorts, is needed to validate our findings in this relatively rare tumor type.
A full-STAC remedy for global digital health transformation: open standards, technologies, architectures and content
Authors: Mehl Garrett L , Seneviratne Martin G , Berg Matt L , Bidani Suhel , Distler Rebecca L , Gorgens Marelize , Kallander Karin E , Labrique Alain B , Landry Mark S , Leitner Carl , Lubell-Doughtie Peter B , Marcelo Alvin B , Matias Yossi , Nelson Jennifer , Nguyen Von , Nsengimana Jean Philbert , Orton Maeghan , Otzoy Garcia Daniel R , Oyaole Daniel R , Ratanaprayul Natschja , Roth Susann , Schaefer Merrick P , Settle Dykki , Tang Jing , Tien-Wahser Barakissa , Wanyee Steven , Hersch Fred 
Published: December 2023

Abstract
The global digital health ecosystem is project-centric: point solutions are developed for vertical health programs and financed through vertical funding allocations. This results in data fragmentation and technology lock-in, compromising health care delivery. A convergence of trends enabled by interoperability and digital governance makes possible a shift towards person-focused health. Together, open Standards, open Technologies, open Architectures and open Content represent a next-generation ‘full-STAC’ remedy for digital health transformation. Local developers and implementers can avoid reinventing the wheel, and instead build digital tools suited to local needs—where data travels with an individual over time, evidence-based practice is easily integrated, and insights are gleaned from harmonized data. This is the culmination of the vision endorsed by 194 WHO Member States in the Global Strategy on Digital Health 2020 to 2025.
Combining wearable fNIRS and immersive virtual reality to study preschoolers’ social development: a proof-of-principle study on preschoolers’ social preference
Authors: Bulgarelli Chiara , Pinti Paola , Aburumman Nadine , Jones Emily J H 
Published: December 2023

AbstractA child’s social world is complex and rich, but has traditionally been assessed with conventional experiments where children are presented with repeated stimuli on a screen. These assessments are impoverished relative to the dynamics of social interactions in real life, and can be challenging to implement with preschoolers, who struggle to comply with strict lab rules. The current work meets the need to develop new platforms to assess preschoolers’ social development, by presenting a unique virtual-reality set-up combined with wearable functional near-infrared spectroscopy (fNIRS). As a proof-of-principle, we validated this platform by measuring brain activity during self-guided social interaction in 3-to-5-year-olds, which is under-investigated, yet crucial to understand the basis of social interactions in preschoolers. 37 preschoolers chose an interaction partner from one of 4 human-like avatars of different gender and age. We recorded spontaneous brain fluctuations from the frontal and temporoparietal regions (notably engaged in social-categorization and preference) while children played a bubble-popping game with a preferred and an assigned avatar. 60% of the participants chose to play with the same-gender and same-age avatar. However, this result was driven by females (&gt;80% vs. 50% in males). Different fronto-temporoparietal connectivity patterns when playing with the two avatars were observed, especially in females. We showed the feasibility of using a novel set-up to naturalistically assess social preference in preschoolers, which was assessed at the behavioural and functional connectivity level. This work provides a first proof-of-principle for using cutting-edge technologies and naturalistic experiments to study social development, opening new avenues of research.
OXIDOSQUALENE CYCLASE 1 and 2 influence triterpene biosynthesis and defense in Nicotiana attenuata
Authors: Yang Caiqiong , Halitschke Rayko , O'Connor Sarah E 
Published: December 2023

Abstract
Triterpenes are a class of bioactive compounds with diverse biological functions, playing pivotal roles in plant defense against biotic stressors. Oxidosqualene cyclases (OSCs) serve as gatekeepers in the biosynthesis of triterpenes. In this study, we utilized a Nicotiana benthamiana heterologous expression system to characterize NaOSC1 from Nicotiana attenuata as a multifunctional enzyme capable of synthesizing lupeol, dammarenediol II, 3-alpha,20-lupanediol, and 7 other triterpene scaffolds. We also demonstrated that NaOSC2 is, in contrast, a selective enzyme, producing only the β-amyrin scaffold. Through virus-induced gene silencing and in vitro toxicity assays, we elucidated the roles of NaOSC1 and NaOSC2 in the defense of N. attenuata against Manduca sexta larvae. Metabolomic and feature-based molecular network analyses of leaves with silenced NaOSC1 and NaOSC2 unveiled 3 potential triterpene glycoside metabolite clusters. Interestingly, features identified as triterpenes within these clusters displayed a significant negative correlation with larval mass. Our study highlights the pivotal roles of NaOSC1 and NaOSC2 from N. attenuata in the initial steps of triterpene biosynthesis, subsequently influencing defense against M. sexta through the modulation of downstream triterpene glycoside compounds.
Incorporating Functional Strength Integration Techniques During Total Hip Arthroplasty Rehabilitation: A Randomized Controlled Trial
Authors: Judd Dana L , Cheuy Victor , Peters Amy , Graber Jeremy , Hinrichs-Kinney Lauren , Forster Jeri E , Christiansen Cory L , Stevens-Lapsley Jennifer E 
Published: December 2023

Abstract

Objective
Total hip arthroplasty (THA) is a common orthopedic procedure that alleviates pain for millions of individuals. Yet, persistent physical function deficits, perhaps associated with movement compensations, are observed after THA. These deficits negatively affect quality of life and health for many individuals. Functional strength integration (FSI) techniques combine muscle strength training with specific movement retraining to improve physical function. This study aimed to determine if FSI would improve functional performance through remediation of movement compensations for individuals after THA.

Methods
A double-blind randomized controlled trial was conducted. Ninety-five participants were randomized to either the FSI or control (CON) group for an 8-week intervention. The FSI protocol included exercise to improve muscular control and stability around the hip to minimize movement compensation during daily activity. The CON protocol included low-load resistance exercise, range-of-motion activities, and patient education. Functional performance, muscle strength, and self-reported outcomes were measured preoperatively, midway and after intervention, and 6 months after THA. Change from preoperative assessment to each time point was measured, and between-group differences were assessed.

Results
There were minimal differences in outcomes between groups at the first postoperative assessment. There were no statistically significant between-group differences in the later assessments, including the primary endpoint. Both groups improved functional outcomes throughout the study period.

Conclusion
The FSI intervention did not result in greater improvements in function after THA compared to the CON intervention. Future work should further investigate additional biomechanical outcomes, timing of the FSI protocol, effective dosing, and patient characteristics predictive of success with FSI.

Impact
Recovery after THA is complex, and individuals after THA are affected by persistent movement deficits that affect morbidity and quality of life. The present study suggests that either approach to THA rehabilitation could improve outcomes for patients, and that structured rehabilitation programs may benefit individuals after THA.
Development and psychometric evaluation of the sexual and reproductive health needs of incarcerated women scale: a methodological study
Authors: Ghareghozloo Mohadeseh , Moridi Maryam , Alimardi Masoud , Safari Kolsoom , Moghadam Zahra Behboodi 
Published: December 2023

Abstract

Background
This study aimed to develop and validate a comprehensive tool designed to assess the sexual and reproductive health (SRH) needs of incarcerated women.

Methods
A methodological study was conducted from January to March 2023 in two female prisons in Tehran and Hamedan, Iran. The participant pool consisted of women who had been incarcerated for a minimum of 6 months and were aged between 15 and 49 years. The instrument development involved two phases. Phase I involved a literature review and interviews with incarcerated women to understand their SRH needs. Phase II assessed psychometric properties to establish the scale’s validity and reliability.

Results
The SRH Needs of Incarcerated Women (SRH-NIW) scale was refined, comprising 37 items across six domains: pregnancy and childbirth services, parenting services, family planning and sexual health services, personal and medical care services, screening services and infection control services. Exploratory factor analysis explained 66.15% of variance. The Content Validity Ratio and Content Validity Index were 0.8 and 0.94, respectively, indicating high content validity. Average Variance Extracted values ranged from 0.592 to 0.698. The whole scale showed a Cronbach’s alpha of 0.823, and the Intra-class Correlation Coefficient was 0.879.

Conclusion
The SRH-NIW scale is a valid and reliable tool for assessing the SRH needs of incarcerated women. It can enhance healthcare services and interventions for incarcerated women, potentially leading to policy improvements within the prison system.
The speed of race
Authors: de Lissa Peter , Schaller Pauline , Caldara Roberto 
Published: December 2023

Abstract
When asked to categorize faces according to ‘race’, people typically categorize other-race faces faster than faces belonging to their own race. This ‘Other Race Categorization Advantage’ is thought to reflect enhanced sensitivity to early visual signals characteristic of other-race faces, and can manifest within 200 ms of face presentation. However, recent research has highlighted the importance of signal intensity in this effect, where visual-degradation of the face images significantly enhances the effect and exposes a behavioural threshold at very low levels of visual quality where other-race visual signals are able to be perceived while same-race signals are not. The current study investigated the effect of signal intensity in race categorization processes in the brain through electroencephalography and in accuracy/reaction times. While replicating the previously observed enhancement of the other-race categorization advantage, we also found enhanced sensitivity to other-race faces in early P1 peaks, as well as later N170 and N250 peaks. These effects, however, related to the varying levels of signal intensity in the face stimuli, suggesting that race categorization may involve different types of perceptual and neural processes rather than one discrete process. The speed at which race is perceived depends on the intensity of the face signal.
Toll-Like Receptor mRNA Levels in Schizophrenia: Association With Complement Factors and Cingulate Gyrus Cortical Thinning
Authors: Weickert Thomas W , Ji Ellen , Galletly Cherrie , Boerrigter Danny , Morishima Yosuke , Bruggemann Jason , Balzan Ryan , O’Donnell Maryanne , Liu Dennis , Lenroot Rhoshel , Weickert Cynthia Shannon , Kindler Jochen 
Published: December 2023

Abstract

Background and Hypotheses
Previous studies revealed innate immune system activation in people with schizophrenia (SZ), potentially mediated by endogenous pathogen recognition receptors, notably Toll-like receptors (TLR). TLRs are activated by pathogenic molecules like bacterial lipopolysaccharides (TLR1 and TLR4), viral RNA (TLR3), or both (TLR8). Furthermore, the complement system, another key component of innate immunity, has previously been linked to SZ.

Study Design
Peripheral mRNA levels of TLR1, TLR3, TLR4, and TLR8 were compared between SZ and healthy controls (HC). We investigated their relationship with immune activation through complement expression and cortical thickness of the cingulate gyrus, a region susceptible to immunological hits. TLR mRNA levels and peripheral complement receptor mRNA were extracted from 86 SZ and 77 HC white blood cells; structural MRI scans were conducted on a subset.

Study Results
We found significantly higher TLR4 and TLR8 mRNA levels and lower TLR3 mRNA levels in SZ compared to HC. TLRs and complemental factors were significantly associated in SZ and HC, with the strongest deviations of TLR mRNA levels in the SZ subgroup having elevated complement expression. Cortical thickness of the cingulate gyrus was inversely associated with TLR8 mRNA levels in SZ, and with TLR4 and TLR8 levels in HC.

Conclusions
The study underscores the role of innate immune activation in schizophrenia, indicating a coordinated immune response of TLRs and the complement system. Our results suggest there could be more bacterial influence (based on TLR 4 levels) as opposed to viral influence (based on TLR3 levels) in schizophrenia. Specific TLRs were associated with brain cortical thickness reductions of limbic brain structures.
Multivariate genome-wide association study of sleep health demonstrates unity and diversity
Authors: Morrison Claire L , Winiger Evan A , Wright Kenneth P , Friedman Naomi P 
Published: December 2023

Abstract
There has been a recent push to focus sleep research less on disordered sleep and more on the dimensional sleep health. Sleep health incorporates several dimensions of sleep: chronotype, efficiency, daytime alertness, duration, regularity, and satisfaction with sleep. A previous study demonstrated sleep health domains correlate only moderately with each other at the genomic level (|rGs| = 0.11–0.51) and show unique relationships with psychiatric domains (controlling for shared variances, duration, alertness, and non-insomnia independently related to a factor for internalizing psychopathology). Of the domains assessed, circadian preference was the least genetically correlated with all other facets of sleep health. This pattern is important because it suggests sleep health should be considered a multifaceted construct rather than a unitary construct. Prior genome-wide association studies (GWASs) have vastly increased our knowledge of the biological underpinnings of specific sleep traits but have only focused on univariate analyses. We present the first multivariate GWAS of sleep and circadian health (multivariate circadian preference, efficiency, and alertness factors, and three single-indicator factors of insomnia, duration, and regularity) using genomic structural equation modeling. We replicated loci found in prior sleep GWASs, but also discovered “novel” loci for each factor and found little evidence for genomic heterogeneity. While we saw overlapping genomic enrichment in subcortical brain regions and shared associations with external traits, much of the genetic architecture (loci, mapped genes, and enriched pathways) was diverse among sleep domains. These results confirm sleep health as a family of correlated but genetically distinct domains, which has important health implications.
Human Hepatocyte Transplantation: Three Decades of Clinical Experience and Future Perspective
Authors: Nulty Jessica , Anand Hanish , Dhawan Anil 
Published: December 2023

Abstract
Orthotopic liver transplantation (OLT) is the current standard of care for both chronic and acute terminal liver disease. However, a major limitation of this treatment is the shortage of healthy donor organs and the need for life-long immunosuppression to prevent graft rejection. Hepatocyte transplantation (HTx) has emerged as a promising, alternative therapeutic approach to either replace OLT or to act as a bridge until a donor liver becomes available thus reducing waiting list mortality. HTx involves the infusion and engraftment of human hepatocytes, typically isolated from organs unsuitable for OLT, into recipient liver parenchyma to carry out the missing hepatic function of the native cells. HTx is less invasive than OLT and can be performed repeatedly if required. The safety of clinical HTx has been shown and treatment results are promising, especially in patients with liver-based metabolic disorders. Nevertheless, HTx has failed to become the standard of care treatment for such disorders. This review aims to evaluate the progress that has been made within the field of HTx over the last 30 years and identify potential shortcomings within the approach which may be hindering its routine clinical application.
Establishment and Validation of a Model for Fetal Neural Ischemia Using Necrotic Core-Free Human Spinal Cord Organoids
Authors: Shin Aeri , Ryu Jae Ryun , Kim Byung Gon , Sun Woong 
Published: December 2023

Abstract
Fetal spinal cord ischemia is a serious medical condition that can result in significant neurological damage and adverse outcomes for the fetus. However, the lack of an appropriate experimental model has hindered the understanding of the pathology and the development of effective treatments. In our study, we established a system for screening drugs that affect fetal spinal cord ischemia using spinal cord organoids. Importantly, we produced necrotic core-free human spinal cord organoids (nf-hSCOs) by reducing the organoid size to avoid potential complications of spontaneous necrosis in large organoids. Exposing nf-hSCOs to CoCl2 as a hypoxia mimetic and hypoglycemic conditions resulted in significant neuronal damage, as assessed by multiple assay batteries. By utilizing this model, we tested chemicals that have been reported to exhibit beneficial effects in brain organoid-based ischemia models. Surprisingly, these chemicals did not provide sufficient benefit, and we discovered that rapamycin is a mild neuroprotective reagent for both axon degeneration and neuronal survival. We propose that nf-hSCO is suitable for large-scale screening of fetal neural ischemia due to its scalability, ease of ischemic induction, implementation of quantifiable assay batteries, and the absence of spontaneous necrosis.
Drought resistance in two populations of invasive Tamarix compared using multiple methods
Authors: Long Randall W , Pratt R Brandon , Jacobsen Anna L 
Published: December 2023

Abstract
An on-going question in plant hydraulic research is whether there is intra-specific variability and/or plasticity in xylem traits. Plasticity could be important in taxa that colonize diverse habitats. We used Tamarix, a non-native woody plant, to investigate population differences in hydraulic conductivity (Ks), vulnerability-to-embolism curves and vessel anatomy. We also conducted a season-long drought experiment to determine water potentials associated with crown dieback of field-grown plants. We measured vessel length and diameter, and compared visual (micro-computed tomography; microCT) and hydraulic methods to quantify percentage loss in hydraulic conductivity (PLC). Among plants grown in a common environment, we did not find differences in our measured traits between two populations of Tamarix that differ in salinity at their source habitats. This taxon is relatively vulnerable to embolism. Within samples, large diameter vessels displayed increased vulnerability to embolism. We found that the microCT method overestimated theoretical conductivity and underestimated PLC compared with the hydraulic method. We found agreement for water potentials leading to crown dieback and results from the hydraulic method. Saplings, grown under common conditions in the present study, did not differ in their xylem traits, but prior research has found difference among source-site grown adults. This suggests that plasticity may be key in the success of Tamarix occurring across a range of habits in the arid southwest USA.