bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Ionospheric Bite‐Outs Between Observations From Beidou GEO TEC and Ionosonde Over East Asia
Authors: Wang Yihan , Huang Fuqing , Lei Jiuhou , Luan Xiaoli , Li Guozhu , Li Na , Dou Xiankang 
Published: November 2023

AbstractIn this study, the daytime bite‐outs were systematically compared between the total electron content (TEC) and peak electron density of F2 layer (NmF2) data using observations from Beidou GEO receivers and ionosondes over East Asia. Moreover, the Thermosphere‐Ionosphere Electrodynamics Global Circulation Model simulations were also employed to analyze the underlying physical mechanisms. There was an obvious difference in the occurrence rate of bite‐outs between TEC and NmF2, although bite‐outs from both data sets showed similar seasonal and latitudinal dependences. Interestingly, the differences in the bite‐out occurrence rates also varied with season and latitude. Their difference, which showed the opposite trend to the occurrence rates themselves, became prominent in equinoxes and less evident in June solstice, peaking at the equatorial ionization anomaly crest. In addition, the occurrence times of bite‐outs were different as observed in TEC and NmF2 sometimes. Bite‐out in TEC tended to start earlier than that in NmF2 at middle latitudes, whereas an opposite pattern was seen at low latitudes. On the contrary, TEC bite‐out often died away earlier than NmF2 bite‐out regardless of the latitudes. The simulations suggested that the vertical E × B drifts associated with zonal equatorial electric fields and the meridional neutral winds could play crucial roles in the characteristics of both bite‐outs.
Variation of Ionospheric Shortwave Absorption of the Extraordinary Wave
Authors: Jiang Xiaoli , Li Huimin , Guo Lixin , Ye Dalin 
Published: November 2023

AbstractIn this study, associating with the A‐H formulation, we have developed a comprehensive ionospheric absorption model by incorporating the latest version of geomagnetic model, the atmospheric and ionospheric model as the background. Based on the absorption model, the ionospheric stratification theory is utilized to explore the trend of the total absorption for extraordinary (X) wave with frequency and elevation angle. We found that the factor of local time has a greater impact on the trend of total absorption variation than other factors—solar activity, season, and latitude, attributing to the variation of electron collision frequency. During the two different local times (daytime and night), the trends of the total absorption have distinct differences. In the daytime, it is worth noting that the reflection heights and the peaks of the absorption coefficient both mainly occur in Layers D and E. Therefore, the non‐deviative absorption dominates the variation of the total absorption. On the other hand, the occurrence of Pedersen ray results in inflexion points arising in the curves of absorption versus frequency or elevation angle. Another interesting phenomenon is the dominant role of deviative absorption at night. At night, the reflection heights mainly occur in Layer F, and the absorption coefficient of Layer F begins to exceed the peak levels of Layers D and E when the frequency or elevation angle is larger than certain values. Hence, the total absorption will grow as a result of the increase in deviative absorption with the increase in reflection height and absorption coefficient.
The Response of Electron Pitch Angle Distributions to the Upper Limit on Stably Trapped Particles
Authors: Walton S. D. , Mann I. R. , Olifer L. , Ozeke L. G. , Forsyth C. , Rae I. J. , Walach M.‐T. , Murphy K. R. , Claudepierre S. G. , Spence H. E. , Baker D. N. 
Published: November 2023

AbstractWe use Van Allen Probes electron data during 70 geomagnetic storms to examine the response of equatorial pitch angle distributions (PADs) at L* = 4.0–4.5 to a theoretical upper limit on stably trapped particle fluxes. Of the energies examined, 54 and 108 keV electron PADs isotropize to a previously assumed level within 6 hr of reaching the limit, near‐identically across all 70 storms, consistent with rapid pitch angle scattering due to chorus wave interactions. In around 30% of events, 54 keV electrons completely exceed the KP limit, before being quickly subdued. 470 and 749 keV PADs show clear indications of an upper limit, though less aligned with the calculated limit used here. The consistency of an absolute upper limit shown across all events demonstrates the importance of this phenomena in both the limiting effect on electron flux and consistently influencing electron PAD evolution during geomagnetic storms. These results also highlight the need for further investigation, particularly related to the limiting of higher energy electrons.
An Assessment of Southern Hemisphere Extratropical Cyclones in ERA5 Using WindSat
Authors: McErlich Cameron , McDonald Adrian , Renwick James , Schuddeboom Alex 
Published: November 2023

AbstractERA5 reanalysis output is compared to WindSat polarimetric microwave radiometer measurements for Southern Hemisphere midlatitude to high‐latitude cyclones between 2003 and 2019. WindSat provides independent measures of low‐level wind speed, total column water vapor (TCWV), cloud liquid water (CLW), and precipitation, which are not assimilated into ERA5. We implement a tracking scheme to identify cyclone centers, before using cyclone composites to match concurrent data in ERA5 and WindSat. We find ERA5 and WindSat show comparable spatial structures for all variables, although their distributions show poorer agreement for CLW and precipitation. Compared to WindSat, ERA5 underestimates TCWV by up to 5% and CLW by up to 40%. ERA5 underestimates precipitation in the warm sector by up to 15%, but overestimates in the cold sector by up to 60%. Similar biases in ERA5 are seen compared to Advanced Microwave Scanning Radiometer for EOS (AMSR‐E) data, even though AMSR‐E radiances are assimilated into ERA5. Comparing ERA5 and WindSat across the cyclone lifecycle, strong spatial correlation is seen as the cyclone deepens and reaches peak intensity, before slightly declining as the cyclone decays. In the cold sector ERA5 shows an underestimation of CLW, yet overestimates precipitation at all lifecycle stages. However, in the warm sector precipitation is underestimated. This potentially suggests biases within the ERA5 parameterizations of cloud and precipitation causing a disconnect between the two. Despite this, ERA5 shows strong correlation with WindSat and determines cyclone structure well across the cyclone lifecycle, showing its value for use in cyclone compositing analysis.
Improving the Estimation of the Diffuse Component of Photosynthetically Active Radiation (PAR)
Authors: Lozano I. , Alados I. , Sánchez‐Hernández G. , Guerrero‐Rascado J. L. , Foyo‐Moreno I. 
Published: November 2023

AbstractMost weather forecasting models are not able to accurately reproduce the great variability existing in the measurements of the diffuse component of photosynthetically active radiation (PAR; 400–700 nm) under all sky conditions. Based on the well‐known relationship between the diffuse fraction (k) and the clearness index (kt), this study addresses improvements in estimations by proposing adaptations of previous models, which were previously applied only to the total solar irradiance (TSI; 280–3,000 nm). In order to reproduce this variability, additional parameters were introduced. The models were tested employing a multisite database gathered at the Mediterranean basin. Since Artificial Neural Network (ANN) models are not limited to fixed coefficients to predict the diffuse fraction of PAR (kPAR), these types of models are more accurate than empirical ones, reaching determination coefficients (r2) up to 0.998. However, the simpler linear model proposed by Foyo‐Moreno et al. (2018), https://doi.org/10.1016/j.atmosres.2017.12.012 shows a similar performance to the ANN models, directly predicting the diffuse component of PAR (PARDiffuse) from TSIDiffuse, with a r2 up to 0.997. Results obtained here also determine that the most important variables for estimating PARDiffuse are kt or kt,PAR, and the apparent solar time (AST). Therefore, PARDiffuse can be modeled using TSI measured in most radiometric stations, reaching r2 up to 0.858 for empirical models and 0.970 for ANN models. This modified approach will allow for the very accurate construction of long‐term data series of PARDiffuse in regions where continuous measurements of PAR are not available.
Mindfulness-based therapy improves brain functional network reconfiguration efficiency
Authors: Yue Wan Lin , Ng Kwun Kei , Koh Amelia Jialing , Perini Francesca , Doshi Kinjal , Zhou Juan Helen , Lim Julian 
Published: November 2023

AbstractMindfulness-based interventions are showing increasing promise as a treatment for psychological disorders, with improvements in cognition and emotion regulation after intervention. Understanding the changes in functional brain activity and neural plasticity that underlie these benefits from mindfulness interventions is thus of interest in current neuroimaging research. Previous studies have found functional brain changes during resting and task states to be associated with mindfulness both cross-sectionally and longitudinally, particularly in the executive control, default mode and salience networks. However, limited research has combined information from rest and task to study mindfulness-related functional changes in the brain, particularly in the context of intervention studies with active controls. Recent work has found that the reconfiguration efficiency of brain activity patterns between rest and task states is behaviorally relevant in healthy young adults. Thus, we applied this measure to investigate how mindfulness intervention changed functional reconfiguration between rest and a breath-counting task in elderly participants with self-reported sleep difficulties. Improving on previous longitudinal designs, we compared the intervention effects of a mindfulness-based therapy to an active control (sleep hygiene) intervention. We found that mindfulness intervention improved self-reported mindfulness measures and brain functional reconfiguration efficiency in the executive control, default mode and salience networks, though the brain and behavioral changes were not associated with each other. Our findings suggest that neuroplasticity may be induced through regular mindfulness practice, thus bringing the intrinsic functional configuration in participants’ brains closer to a state required for mindful awareness.
Soft tissue expansion using self-inflating osmotic hydrogel expanders prior to bone augmentation: healing and complications. Evidence-based review
Authors: Ellesøe Adam Gade , Shado Rawand , Pereira Ines Novo , Madruga David , Hassan Haidar 
Published: November 2023

AbstractAimThis review aims to assess complication rates, soft tissue gain, and bone gain associated with the use of self-inflating osmotic hydrogel tissue expanders (SOHTEs) for soft tissue expansion (STE).MethodsA comprehensive search on Pubmed and Google Scholar databases was conducted to identify human studies using SOHTEs for STE; last searched in March 2023. Expansion phase details and expander variables were documented. Complication rates, soft tissue gain, and bone gain reported in each study were also recorded. The inclusion criteria encompassed human studies ranging from evidence levels II–IV (Oxford Centre for Evidence-Based Medicine Levels of Evidence), without specific date limits. For assessing bias in randomized controlled trials (RCTs), a Risk of Bias tool was employed. The synthesised results were presented through tables, sunburst plots, and bar charts.ResultsA total of 13 studies were identified, comprising 4 RCTs, 1 cohort study, and 8 case-series. Employment of SOHTEs yielded an overall complication rate of 17% (24/140 sites), with expander perforation accounting for 9.3% (13/140) of the sites. Specific complication rates included dehiscence (1.4%, 2/140 sites), paraesthesia (1.4%, 2/140 sites), and infection (1.4%, 2/140 sites). All randomized controlled trials (RCTs) were categorised at Level II. The remaining investigations primarily consisted of Level IV case-series lacking controls. All studies demonstrated some concerns towards bias.ConclusionSTE studies using SOHTEs exhibit a reduction in complications associated with bone augmentation in scenarios of inadequate soft tissue coverage. Preliminary evidence suggests potential benefits even in cases with sufficient soft tissue. Adherence to procedural precautions may reduce the risk of expander perforations, further diminishing complications. Subsequent studies should incorporate individual patient and expander variables in their reports to explore the impact of expansion phases on complication rates, as well as bone and soft tissue augmentation.
Integration of FUNDC1-associated mitochondrial protein import and mitochondrial quality control contributes to TDP-43 degradation
Authors: Ma Jinfa , Liu Lei , Song Lu , Liu Jianghong , Yang Lingyao , Chen Quan , Wu Jane Y. , Zhu Li 
Published: November 2023

AbstractThough TDP-43 protein can be translocated into mitochondria and causes mitochondrial damage in TDP-43 proteinopathy, little is known about how TDP-43 is imported into mitochondria. In addition, whether mitochondrial damage is caused by mitochondrial mislocalization of TDP-43 or a side effect of mitochondria-mediated TDP-43 degradation remains to be investigated. Here, our bioinformatical analyses reveal that mitophagy receptor gene FUNDC1 is co-expressed with TDP-43, and both TDP-43 and FUNDC1 expression is correlated with genes associated with mitochondrial protein import pathway in brain samples of patients diagnosed with TDP-43 proteinopathy. FUNDC1 promotes mitochondrial translocation of TDP-43 possibly by promoting TDP-43-TOM70 and DNAJA2-TOM70 interactions, which is independent of the LC3 interacting region of FUNDC1 in cellular experiments. In the transgenic fly model of TDP-43 proteinopathy, overexpressing FUNDC1 enhances TDP-43 induced mitochondrial damage, whereas down-regulating FUNDC1 reverses TDP-43 induced mitochondrial damage. FUNDC1 regulates mitochondria-mediated TDP-43 degradation not only by regulating mitochondrial TDP-43 import, but also by increasing LONP1 level and by activating mitophagy, which plays important roles in cytosolic TDP-43 clearance. Together, this study not only uncovers the mechanism of mitochondrial TDP-43 import, but also unravels the active role played by mitochondria in regulating TDP-43 homeostasis.
MELK promotes HCC carcinogenesis through modulating cuproptosis-related gene DLAT-mediated mitochondrial function
Authors: Li Zhipeng , Zhou Huaxin , Zhai Xiangyu , Gao Lin , Yang Mengfan , An Baokun , Xia Tong , Du Gang , Li Xiaoming , Wang Wei , Jin Bin 
Published: November 2023

AbstractCuproptosis caused by copper overload is mediated by a novel regulatory mechanism that differs from previously documented mechanisms regulating cell death. Cells dependent on mitochondrial respiration showed increased sensitivity to a copper ionophore elesclomol that induced cuproptosis. Maternal embryonic leucine zipper kinase(MELK) promotes tumorigenesis and tumor progression through the PI3K/mTOR pathway, which exerts its effects partly by targeting the pyruvate dehydrogenase complex(PDHc) and reprogramming the morphology and function of mitochondria. However, the role of MELK in cuproptosis remains unclear. Here, we validated that elevated MELK expression enhanced the activity of PI3K/mTOR signaling and subsequently promoted Dihydrolipoamide S-Acetyltransferase (DLAT) expression and stabilized mitochondrial function. This regulatory effect helped to improve mitochondrial respiration, eliminate excessive intracellular reactive oxygen species (ROS), reduce intracellular oxidative stress/damage and the possibility of mitochondria-induced cell fate alternations, and ultimately promote the progression of HCC. Meanwhile, elesclomol reduced translocase of outer mitochondrial membrane 20(TOM 20) expression and increased DLAT oligomers. Moreover, the above changes of MELK to HCC were abolished by elesclomol. In conclusion, MELK enhanced the levels of the cuproptosis-related signature(CRS) gene DLAT (especially the proportion of DLAT monomer) by activating the PI3K/mTOR pathway, thereby promoting elesclomol drug resistance, altering mitochondrial function, and ultimately promoting HCC progression.
The roles of m6A methylation in cervical cancer: functions, molecular mechanisms, and clinical applications
Authors: Mao Zhonghao , Wang Bingyu , Zhang Teng , Cui Baoxia 
Published: November 2023

AbstractCervical cancer (CC) is a gynecological neoplasm with the highest incidence rate, primarily attributed to the persistent infection of high-risk Human papillomavirus (HPV). Despite extensive research, the pathogenesis of CC remains unclear. N6-methyladenosine (m6A) methylation, the most prevalent form of epigenetic modification in RNA, is intricately linked to cell proliferation, metastasis, metabolism, and therapeutic resistance within the tumor microenvironment (TME) of CC. The involvement of the writer, reader, and eraser in m6A modification impacts the advancement of tumors through the regulation of RNA stability, nuclear export, translation efficiency, and RNA degradation. Here, we discuss the biogenesis of m6A, the atypical expressions of m6A regulators, the mechanisms of molecular interactions, and their functions in CC. Furthermore, we elucidate m6A modification of non-coding RNA. In the context of precision medicine, and with the advancements of genomics, proteomics, and high-throughput sequencing technologies, we summarize the application of m6A in the clinical diagnosis and treatment of CC. Additionally, new perspectives on detection methods, immune regulation, and nano-drug development are presented, which lay the foundation for further research of m6A and provide new ideas for the clinical treatment of CC.
An on-demand bioresorbable neurostimulator
Authors: Lee Dong-Min , Kang Minki , Hyun Inah , Park Byung-Joon , Kim Hye Jin , Nam Soo Hyun , Yoon Hong-Joon , Ryu Hanjun , Park Hyun-moon , Choi Byung-Ok , Kim Sang-Woo 
Published: November 2023

AbstractBioresorbable bioelectronics, with their natural degradation properties, hold significant potential to eliminate the need for surgical removal. Despite notable achievements, two major challenges hinder their practical application in medical settings. First, they necessitate sustainable energy solutions with biodegradable components via biosafe powering mechanisms. More importantly, reliability in their function is undermined by unpredictable device lifetimes due to the complex polymer degradation kinetics. Here, we propose an on-demand bioresorbable neurostimulator to address these issues, thus allowing for clinical operations to be manipulated using biosafe ultrasound sources. Our ultrasound-mediated transient mechanism enables (1) electrical stimulation through transcutaneous ultrasound-driven triboelectricity and (2) rapid device elimination using high-intensity ultrasound without adverse health effects. Furthermore, we perform neurophysiological analyses to show that our neurostimulator provides therapeutic benefits for both compression peripheral nerve injury and hereditary peripheral neuropathy. We anticipate that the on-demand bioresorbable neurostimulator will prove useful in the development of medical implants to treat peripheral neuropathy.
Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models
Authors: Wei Tuo , Sun Yehui , Cheng Qiang , Chatterjee Sumanta , Traylor Zachary , Johnson Lindsay T. , Coquelin Melissa L. , Wang Jialu , Torres Michael J. , Lian Xizhen , Wang Xu , Xiao Yufen , Hodges Craig A. , Siegwart Daniel J. 
Published: November 2023

AbstractApproximately 10% of Cystic Fibrosis (CF) patients, particularly those with CF transmembrane conductance regulator (CFTR) gene nonsense mutations, lack effective treatments. The potential of gene correction therapy through delivery of the CRISPR/Cas system to CF-relevant organs/cells is hindered by the lack of efficient genome editor delivery carriers. Herein, we report improved Lung Selective Organ Targeting Lipid Nanoparticles (SORT LNPs) for efficient delivery of Cas9 mRNA, sgRNA, and donor ssDNA templates, enabling precise homology-directed repair-mediated gene correction in CF models. Optimized Lung SORT LNPs deliver mRNA to lung basal cells in Ai9 reporter mice. SORT LNP treatment successfully corrected the CFTR mutations in homozygous G542X mice and in patient-derived human bronchial epithelial cells with homozygous F508del mutations, leading to the restoration of CFTR protein expression and chloride transport function. This proof-of-concept study will contribute to accelerating the clinical development of mRNA LNPs for CF treatment through CRISPR/Cas gene correction.
Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy
Authors: Guo Yuedong , Bao Qunqun , Hu Ping , Shi Jianlin 
Published: November 2023

AbstractPro-tumoral macrophages in lung tumors present a significant challenge in immunotherapy. Here, we introduce a pH-responsive nanomedicine approach for activating anti-tumoral macrophages and dendritic cells. Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and a CD47 inhibitor (RRX-001) into lung tumors. In the tumor acidic environment, TTA-Q6 is released, disrupting cancer cell calcium uptake, causing endoplasmic reticulum stress and inducing calreticulin transfer to the cell surface. Surface calreticulin activates macrophages and triggers dendritic cell maturation, promoting effective antigen presentation and therefore activating antitumor T cells. Simultaneously, RRX-001 reduces CD47 protein levels, aiding in preventing immune escape by calreticulin-rich cancer cells. In lung tumor models in male mice, this combined approach shows anti-tumor effects and immunity against tumor re-exposure, highlighting its potential for lung cancer immunotherapy.
A genomic catalogue of soil microbiomes boosts mining of biodiversity and genetic resources
Authors: Ma Bin , Lu Caiyu , Wang Yiling , Yu Jingwen , Zhao Kankan , Xue Ran , Ren Hao , Lv Xiaofei , Pan Ronghui , Zhang Jiabao , Zhu Yongguan , Xu Jianming 
Published: November 2023

AbstractSoil harbors a vast expanse of unidentified microbes, termed as microbial dark matter, presenting an untapped reservoir of microbial biodiversity and genetic resources, but has yet to be fully explored. In this study, we conduct a large-scale excavation of soil microbial dark matter by reconstructing 40,039 metagenome-assembled genome bins (the SMAG catalogue) from 3304 soil metagenomes. We identify 16,530 of 21,077 species-level genome bins (SGBs) as unknown SGBs (uSGBs), which expand archaeal and bacterial diversity across the tree of life. We also illustrate the pivotal role of uSGBs in augmenting soil microbiome’s functional landscape and intra-species genome diversity, providing large proportions of the 43,169 biosynthetic gene clusters and 8545 CRISPR-Cas genes. Additionally, we determine that uSGBs contributed 84.6% of previously unexplored viral-host associations from the SMAG catalogue. The SMAG catalogue provides an useful genomic resource for further studies investigating soil microbial biodiversity and genetic resources.