bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient-derived organoids recapitulate glioma-intrinsic immune program and progenitor populations of glioblastoma
Authors: Watanabe Fumihiro , Hollingsworth Ethan W , Bartley Jenna M , Wisehart Lauren , Desai Rahil , Hartlaub Annalisa M , Hester Mark E , Schiapparelli Paula , Quiñones-Hinojosa Alfredo , Imitola Jaime 
Published: February 2024

Abstract
Glioblastoma multiforme (GBM) is a highly lethal human cancer thought to originate from a self-renewing and therapeutically-resistant population of glioblastoma stem cells (GSCs). The intrinsic mechanisms enacted by GSCs during 3D tumor formation, however, remain unclear, especially in the stages prior to angiogenic/immunological infiltration. In this study, we performed a deep characterization of the genetic, immune, and metabolic profiles of GBM organoids from several patient-derived GSCs (GBMO). Despite being devoid of immune cells, transcriptomic analysis across GBMO revealed a surprising immune-like molecular program, enriched in cytokine, antigen presentation and processing, T-cell receptor inhibitors, and interferon genes. We find two important cell populations thought to drive GBM progression, Special AT-rich sequence-binding protein 2 (SATB2+) and homeodomain-only protein homeobox (HOPX+) progenitors, contribute to this immune landscape in GBMO and GBM in vivo. These progenitors, but not other cell types in GBMO, are resistant to conventional GBM therapies, temozolomide and irradiation. Our work defines a novel intrinsic immune-like landscape in GBMO driven, in part, by SATB2+ and HOPX+ progenitors and deepens our understanding of the intrinsic mechanisms utilized by GSCs in early GBM formation.
Modelling the longer-term health and health inequality impacts of changes in alcohol consumption during the COVID-19 pandemic in England
Authors: Angus Colin , Henney Madeleine , Pryce Robert 
Published: February 2024

Abstract

Background
Alcohol consumption changed substantially during the COVID-19 pandemic for many people. This study quantified how these changes in drinking varied across the population and their potential longer-term impact on health and health inequalities.

Methods
We analyzed data from the Alcohol Toolkit Study to estimate how alcohol consumption changed during the pandemic (April 2020–November 2021) and how these changes varied with age, sex, drinking level and socioeconomic position. We combined these estimates with a range of alternative scenarios of future alcohol consumption and used the Sheffield Alcohol Policy Model to assess the long-term health and health inequality impacts of these changes.

Results
Alcohol consumption in 2020–21 increased in heavier drinkers but fell in moderate drinkers. If alcohol consumption returns to pre-pandemic levels in 2022, we estimate a total of 42 677 additional hospital admissions and 1830 deaths over 20 years because of these changes. If consumption remains at 2021 levels in the long-term these figures rise to 355 832 and 12 849, respectively. In all scenarios, the biggest increase in harm occurs in the most deprived 20% of the population.

Conclusions
Pandemic-era changes in alcohol consumption are likely to have a significant negative impact on public health and health inequalities, even under optimistic assumptions about future drinking.
Latency to N3 interruption in arousal disorders
Authors: Perretti Carlos , Gales Ana , Leu-Semenescu Smaranda , Dodet Pauline , Bianquis Clara , Groos Elisabeth , Puligheddu Monica , Maranci Jean-Baptiste , Arnulf Isabelle 
Published: February 2024

Abstract

Study Objectives
To help expert witnesses in criminal cases using the “sleepwalking defense,” we studied the time of first and last interruptions from stage N3 in patients with arousal disorders, including sexsomnia, as well as their determinants.

Methods
The epochs of lights off, sleep onset, first N3 interruption (with and without behaviors), and last N3 interruption were determined by videopolysomnography on two consecutive nights in 163 adults with disorders of arousal, including 46 with and 117 without sexsomnia.

Results
The first N3 interruption (independently of concomitant behavior) occurred as early as 8 minutes after sleep onset and within 100 minutes of falling asleep in 95% of cases. The first motor arousal from N3 occurred as early as 25 minutes after lights off time, a timing more variable between participants (between 30 and 60 minutes after lights off time in 25% of participants and within 60 minutes of falling asleep in 50%). These latencies did not differ between the groups with and without sexsomnia. No correlation was found between these latencies and the young age, sex, or clinical severity. The latency of motor arousals was shorter when they were associated with a fast-wave EEG profile and were not preceded by another type of N3 arousal.

Conclusions
The first motor arousal may occur early in the night in patients with arousal disorders, with or without sexsomnia, suggesting that abnormal behaviors occurring as early as 25 minutes after lights off time in clinical and criminal cases can be a parasomnia manifestation.
Electrification of urban road traffic: a reliability analysis of traction power supply for electric road systems
Authors: Zuo Wei , Li Kang 
Published: February 2024

Abstract
The electric road system (ERS) is an emerging technology to support decarbonization of heavy goods vehicles. The reliability of the traction power supply system (TPSS) for an electric road is a key consideration in ERS planning and maintenance, but since the development of ERS is still at its early stage, the reliability study of different ERS systems has not been fully researched. This paper fills this research gap. It first analyses the potential operation risks of three different ERS systems using the failure mode and effect analysis method. Then, a fault tree model is built to analyse the overall reliability of the traction power supply system of each of the three ERS types and to identify the weak links. Furthermore, the overall failure rate and reliability indicators are obtained by combining the possible failures of both the electric and non-electric components of the TPSS. Finally, based on the analysis of the potential risks and the corresponding reliability indicators, the advantages and disadvantages of different ERS solutions and the future development prospects are discussed.
Old Foe, New Host: Epidemiology, Genetic Diversity, and Pathogenic Characterization of Maize Streak Virus in Rice Fields from Burkina Faso
Authors: Fouad Noun Magdy Ibrahim , Barro Mariam , Bangratz Martine , Sérémé Drissa , Filloux Denis , Fernandez Emmanuel , Julian Charlotte , Saïbou Nignan , Kassankogno Abalo Itolou , Guigma Abdoul Kader , Roumagnac Philippe , Wonni Issa , Tollenaere Charlotte , Poulicard Nils 
Published: February 2024

Rice is of critical significance regarding food security worldwide, including in Africa. Only two viruses impacting rice production in Africa have been deeply investigated for decades: the rice yellow mottle virus ( Solemoviridae) and the rice stripe necrosis virus ( Benyviridae). Using viral metagenomics, we aimed at broadening knowledge on interacting communities associated with plants in rice landscapes and exploring the diversity and epidemiological status of viruses circulating in rice fields from Burkina Faso. We performed an epidemiological survey in this country between 2016 and 2019 involving 57 small farmers’ rice fields under two production systems: rainfed lowlands and irrigated areas. More than 2,700 rice samples were collected without regard to disease symptoms following a regular scheme. Wild and cultivated Poaceae (maize and sugarcane) growing in nearby rice fields were also collected. Unexpectedly, metagenomics detected maize streak virus (MSV) ( Geminiviridae) in analyzed rice samples. Further molecular analyses using rolling circle amplification-polymerase chain reaction showed that MSV was widely distributed and highly prevalent in both rainfed lowlands and irrigated rice areas. MSV-A and MSV-G strains were identified. MSV-G, exclusively identified thus far in wild grasses, was the most prevalent strain, whereas MSV-A, known to cause severe symptoms in maize, was sporadically identified. Using infectious clones in experimental conditions, we confirmed the pathogenicity of both MSV strains in rice. Thus, in addition to contributing to the epidemiological surveillance of rice production in Africa, our results illuminate new epidemiological and pathogenic aspects of one of the most studied plant viruses with significant economic consequences in Africa. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Neoadjuvant Chemoradiotherapy Upregulates the Immunogenicity of Cold to Hot Tumors in Esophageal Cancer Patients
Authors: Nagaki Yushi , Motoyama Satoru , Sato Yusuke , Wakita Akiyuki , Fujita Hiromu , Kemuriyama Kohei , Sasamori Ryohei , Nozaki Shu , Nomura Kyoko , Minamiya Yoshihiro 
Published: February 2024

Objective:
To test the hypothesis that neoadjuvant chemoradiotherapy (NACRT) is more effective against hot esophageal squamous cell carcinoma (ESCC) and that it may upregulate tumor immunogenicity.

Background:
There have been several recent reports showing the efficacy of immune check-point inhibitors (ICIs) against esophageal cancer, especially immunologically hot tumors. In addition, several studies have suggested that chemotherapy and radiotherapy may convert cold tumors to hot tumors.

Methods:
Of 105 ESCC patients who underwent surgery after NACRT between 2010 and 2018 at our hospital, 99 whose biopsy tissue samples were obtained were enrolled. Based on immunohistochemical analysis, tumors that were FOXA1 (+) and/or EYA2 (+) were defined as hot tumors, others were cold tumors. We then investigated the association between tumor immunogenicity and clinicopathological features.

Results:
The 29 patients with hot tumors before NACRT had a significantly better 5-year disease-specific survival (DSS) rate than the remaining 70 patients with cold tumors (85% vs 64%; P = 0.036). In a multivariate analysis, tumor immunogenicity was a significant independent predictor of DSS. Of 68 patients without a pathological complete response (non-pCR) in their primary tumor, 51 (75%) had hot tumors after NACRT. Moreover, 75% (36/48) of tumors that were cold before NACRT were converted to hot tumors after NACRT.

Conclusions:
Patients with hot ESCC tumors treated with NACRT plus esophagectomy had a better prognosis than those with cold tumors. NACRT upregulated cold tumor immunogenicity to hot tumors, suggesting NACRT may increase the sensitivity of ESCC to adjuvant ICIs.
Measuring external rotation of the fibula and fibular length in bilateral CT scans: how reliable is this method?
Authors: Penning Diederick , Molendijk Juul , Halm Jens A. , Schepers Tim 
Published: February 2024

Objective.
During ankle fracture surgery, goals include accurate reduction and fixation of the tibiofibular joint and fibular length. Bilateral postoperative computed tomography (CT) can be performed to assess syndesmotic reduction, the talar dome angle and fibular length.. The study aim is to compare side-to-side differences of the fibular rotation and fibular length using bilateral CT’s of uninjured ankles.

Methods.
We included patients with bilateral CT scans of uninjured ankles and measured external rotation using the Nault talar dome method and fibular length using the coronal method of Prior et al. The average, difference, and ratio (injured side/healthy side) and inter-observer variability were calculated.

Results.
There were 83 patients included (166 ankles, mean age 47 years, 77.1% male). A random set of 66 ankles (33 CT scans) were used to measure inter-observer variability. The mean degrees of external rotation ranged from 6.6 to 7.7, mean difference from 1.4 to 3.4 degrees, mean ratio from 1.1 to 1.5, inter-observer variability from 0.27 to 0.65. For fibular length, the mean ranged from 24.6 to 25.8 mm, mean difference in fibular length ranged from 0.5 to 2.1 mm, mean ratio ranged from 1.0 to 1.1 mm and inter-observer variability ranged from 0.45 to 0.73.

Conclusion.
Using bilateral ankle CTs, mean differences in fibular rotation using the Nault talar dome method were 1.4 to 3.4 degrees. The distal fibular length had a mean difference between both sides of 0.5 to 2.1mm. Even though the ICC’s were low, the inter-leg differences between patients were small, making them useful for clinical practice.

Level of evidence.
Diagnostic level III. See Instructions for Authors for a complete description of levels of evidence.
Metagenomic Next-generation Sequencing for Pathogen Identification in Bronchoalveolar Lavage Fluid From Neonates Receiving Extracorporeal Membrane Oxygenation
Authors: Zheng Yi-Rong , Chen Xiu-Hua , Chen Qiang , Cao Hua 
Published: February 2024

Background:
Neonates on extracorporeal membrane oxygenation (ECMO) are at high risk of infection. Rapid and accurate identification of pathogens is essential to improve the prognosis of children on ECMO. Metagenome next-generation sequencing (mNGS) has been used in recent years to detect pathogenic bacteria, but evidence for its use in neonates on ECMO is lacking.

Methods:
This retrospective study was conducted using an electronic medical record system. We analyzed the results of mNGS and conventional microbiological tests (CMTs) in bronchoalveolar lavage fluid of neonates receiving ECMO support with pulmonary infections in our hospital from July 2021 to January 2023.

Results:
We screened 18 ECMO-supported neonates with pneumonia for inclusion in the study. The median age of the included children was 2 (1–4) days, the median gestational age was 38.3 (33–40+4) weeks, and the median weight was 3.3 (2.2–4.8) kg. The detection rate of mNGS was 77.8% (14/18), higher than the 44.4% (8/18) of CMT (P = 0.04). A total of 20 pathogens were detected in mNGS, with the top 3 most common pathogens being Klebsiella pneumoniae, Acinetobacter baumannii and Escherichia coli. Mixed infections were found in 14 cases (77.8%), including 13 cases (72.2%) with mixed infections detected by mNGS and 7 cases (27.8%) with mixed infections detected by CMT. A total of 9 children underwent treatment changes based on mNGS results and all of them experienced relief of symptoms.

Conclusion:
Compared with CMT, mNGS can detect pathogens earlier and more sensitively, and may play an important role in ECMO-supported neonatal pneumonia pathogen detection and optimization of antibiotic therapy.
Application of panoramic radiographs in the diagnosis of temporomandibular disorders
Authors: Meng Xianbin , Liu Sen , Wu Zg , Guo Liangying 
Published: February 2024

To evaluate the feasibility of temporomandibular disorder (TMD) diagnosis with panoramic radiography, and provide standardized data for artificial intelligence-assisted diagnosis by measuring the differences in the condylar and mandibular ramus heights. A total of 500 panoramic radiographs (219 male and 281 female participants) of healthy individuals were examined. The panoramic machine compatible measurement software, SCANORA 5.2.6, was used to measure the bilateral condylar height and mandibular ramus height, and SPSS 27.0 was used to calculate the left- and right-side differences in condylar height and mandibular ramus height of healthy individuals. Magnetic resonance images of the temporomandibular joint region obtained from 46 outpatients in the Stomatology Department were selected along with their corresponding panoramic radiographs. The left- and right-sided differences were measured and compared with the magnetic resonance imaging results. The measurement data are expressed as mean ± standard deviation (mm). t Tests were used to analyze data from healthy male and healthy female groups. The findings revealed that while there was no significant difference (P > .05) in the height of the condyle between men and women, there was a significant difference (P
< .05) in the height of the mandibular ramus. In healthy population, the difference in height between the left and right condyle was 1.09 ± 0.99 mm. The difference in height of mandibular ramus in men was 1.26 ± 0.85 mm and that in women was 1.19 ± 0.87 mm. For the diagnosis of TMD, the sensitivity of panoramic radiographs was 94.74% (36/38), specificity was 75.00% (6/8), and diagnostic accuracy was 91.30% (42/46). The height of the right and left lateral condyles was not identical in healthy individuals, resulting in a discernible height discrepancy. In addition, the height of the mandibular ramus varied. By considering the left-right lateral height differences identified in this study along with clinical examination, it is possible to employ this metric as a preliminary screening tool for patients with TMD. Further, the use of panoramic radiographs for initial TMD screening is both viable and significant.
Arthroscopic treatment of osteoid osteoma in the posterior proximal tibia: A case report and literature review
Authors: He Yinhao , Li Xiaosheng , Tu Zhi-Xing , Chen Hong-Wen , Zeng Hui , Peng Qiang , Chen Tie-Zhu 
Published: February 2024

Background:
Osteoid osteoma (OO) is a benign lesion characterized by an increased fibrous component in the bone marrow, presence of bone-like structures within the medullary cavity, and a surrounding sclerotic bone rim. Reports on OO located in the posterior proximal tibia are rare.

Case summary:
Herein, we report the case of an 18-year-old male, admitted for the evaluation of right knee pain. The right knee pain had started 6 months prior without any apparent cause, which was notably severe at night, affecting sleep, and was exacerbated while climbing stairs or bearing weight. The patient also experienced pain on flexion. Three-dimensional computed tomography and magnetic resonance imaging revealed a nodular lesion beneath the cortical bone of the posterior medial plateau of the right tibia and an abnormal signal focus on the posterior lateral aspect of the right tibial plateau associated with extensive bone marrow edema. A small amount of fluid was present in the right knee joint capsule. The patient subsequently underwent arthroscopic excision of the OO. Postoperatively, there was significant relief of pain, and the knee range of motion returned to normal.

Conclusion:
Although OO in the posterior proximal tibia is a rare occurrence, it can be effectively excised through minimally invasive arthroscopic visualization.
Infiltration of nanocarbon into the trachea during thyroidectomy surgery: case series and literature review
Authors: Li Wu 
Published: February 2024

Introduction:
The presence of metastatic disease in the cervical lymph nodes can affect the recurrence and survival of patients with thyroid cancer. Parathyroid gland injury during surgery can result in hypoparathyroidism, particularly with total thyroidectomy. Injection of carbon nanoparticles into the thyroid will label draining lymph nodes and aid in the visualization of metastatic cervical lymph nodes during a radical operation, sparing accidental damage to the parathyroid glands. Although reported to be useful during surgery, the safety of nanocarbon particles has rarely been investigated, and adverse side effects need to be studied.

Case presentation:
The author describes five patients with thyroid cancer who had carbon secretions in the trachea or mucosa after carbon nanoparticles were injected into the thyroid. A patient with carbon secretions in the trachea mucosa recovered but had progressive dyspnoea. Surgical treatment was performed, and a mass was found in the trachea mucosa. After excluding all other possibilities, the author concluded that the mass was caused by nanocarbon suspension.

Discussion:
To the author’s knowledge, there are no reports on nanocarbon suspension into the mucosa and no consensus has yet been reached on the precise injection site, depth, or dose for injecting carbon nanoparticles before thyroidectomy.

Conclusion:
The author suggests that the most appropriate injection depth of nanocarbon suspensions should be no more than 3 mm of the thyroid gland thickness to avoid deep injection into the trachea.
Global research productivity of post-transplant lymphoproliferative disorder: a bibliometric study
Authors: Rawashdeh Badi , AlRyalat Saif Aldeen , Rawshdeh Aasem , Abuassi Mohammad , Al-mansour Zeina , El-Hinnawi Ashraf 
Published: February 2024

Background:
Post-transplant lymphoproliferative diseases (PTLD) are a heterogeneous collection of neoplasms that occur after solid organ transplants (SOT). In the past 20 years, there has been a rise in PTLD research. This study aims to investigate the global research output and interest regarding PTLD using a bibliometric approach.

Material and methods:
On 28 November 2022, the Web of Science Core Collection documents on PTLD published between 2000 and 2022 were collected and analyzed using bibliometric techniques. The VOSviewer application was utilized to visualize the annual number of publications, authors, organizations, countries, published journals, citations, and most occurring keywords.

Results:
A total of 2814 documents were retrieved, and a screening process included 1809 documents. The total number of citations was 45 239, and the average number per item was 25. Most articles (n = 747) and citations (n = 25 740) were produced in the United States. Based on citations, most of the top 10 institutions that contributed were in the United States of America. The University of Pittsburgh topped the list with 2700 citations and 64 articles. The vast majority of articles were published in Pediatric Transplantation (n = 147), Transplantation (n = 124), and the American Journal of Transplantation (n = 98). Transplantation has received the most citations, 6499, followed by the American Journal of Transplantation with 5958 citations and Blood with 4107 citations.

Conclusion:
With ongoing debates over optimal classification, Epstein-Bar virus involvement, and treatment, this topic has received significant interest from researchers in recent years. Our results can be used as a guide for future research in the field and as a framework for a more in-depth look at the scientific progress of PTLD.