bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights into the mechanism of L-malic acid on drip loss of chicken meat under commercial conditions
Authors: Sun Haijun , Yan Xue , Wang Lu , Zhu Ruimin , Chen Meixia , Yin Jingdong , Zhang Xin 
Published: January 2024

Abstract
Background
A deterioration in the meat quality of broilers has attracted much more attention in recent years. L-malic acid (MA) is evidenced to decrease meat drip loss in broilers, but the underlying molecular mechanisms are still unclear. It’s also not sure whether the outputs obtained under experimental conditions can be obtained in a commercial condition. Here, we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale rearing.

Results
Results showed that the growth performance and drip loss were improved by MA supplementation. Meat metabolome revealed that L-2-aminoadipic acid, β-aminoisobutyric acid, eicosapentaenoic acid, and nicotinamide, as well as amino acid metabolism pathways connected to the improvements of meat quality by MA addition. The transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response, evidenced by the enhanced B cell receptor signaling pathway, NF-κB signaling pathway, TNF signaling pathway, and IL-17 signaling pathway.

Conclusions
We provided evidence that MA decreased chicken meat drip loss under commercial conditions. Metabolome and transcriptome revealed a comprehensive understanding of the underlying mechanisms. Together, MA could be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.
Congenital septal defects in Karachi, Pakistan: an update of mutational screening by high-resolution melting (HRM) analysis of MTHFR C677T
Authors: Ali Syed Irtiza , Khan Obaid Yusuf , Naveed Nadir , Ahmad Hussain , Patel Najma , Arif Afsheen 
Published: January 2024

Abstract
Background
Congenital heart defects (CHDs) are the heart structural malformations present at birth. Septal defects account for 40% of CHD, including atrial, ventricular and atrioventricular septal defects. In Pakistan, the prevalence of CHD is 3.4 in 1000, and a study estimated that 60,000 babies are born with CHD annually. Methylenetetrahydrofolate reductase (MTHFR), a chief enzyme, involved in the folate metabolism. The missense mutation, C677T (rs1801133), exists in MTHFR gene, results in a MTHFR thermolabile variant having low enzymatic activity. The study is aim to identify the MTHFR C677T variant association with septal defects.

Methods
Samples of 194 CHD patients (age $$\overline{X }\hspace{0.17em}$$

X
¯

= 5.8 ± 5.1) and 50 normal echo controls (age $$\overline{X }\hspace{0.17em}$$

X
¯

= 6.0 ± 4.9), confirmed by pediatric consultant, were collected. Extracted DNA, quantified by agarose gel electrophoresis and nanodrop, was screened for SNP by high-resolution melting (HRM). Further, HRM results were confirmed using restriction analysis and sequencing. HRM was simply and precisely genotyped the samples within 3 h at low cost.

Results
Genotypic data suggested that heterozygous mutant (CT) was frequent in congenital septal defect patients (0.26) which was higher than controls (0.143), p > 0.05. Mutant (TT) genotype was not found in this study.

Conclusions
rs1801133 has lack of significant association with congenital septal defects. The absence of TT genotype in this study suggesting the role of natural selection in targeted population. HRM is an easy, fast and next generation of PCR, which may be used for applied genomics.
Host plants directly determine the α diversity of rhizosphere arbuscular mycorrhizal fungal communities in the National Tropical Fruit Tree Field Genebank
Authors: Wang Jugang , Gao Xiaomin , Wang Jiali , Song Jian , Zhu Zhoujun , Zhao Junru , Liu Min , Wang Zuhua 
Published: January 2024

AbstractArbuscular mycorrhizal fungi (AMF) typically exhibit reduced diversity in nutrient-rich soils. However, whether the influence of host plants on AMF within the rhizosphere is direct or indirect through the alteration of soil nutrient levels has yet to be determined. This study explored the diversity and colonization of AMF in the National Tropical Fruit Tree Genebank, where chemical fertilizers are used to preserve minor tropical fruit germplasms. We aimed to understand the direct and indirect effects of host plants on AMF community dynamics, sporulation, and colonization. By analyzing fine roots and rhizosphere soil from 12 tropical fruit trees, we assessed mycorrhizal colonization indices, soil nutrients, AMF spore density, and community structure. Despite their low colonization density and arbuscular abundance, all the tree roots harbored AMF, with spore densities ranging from 24.00 to 204.80 spores/100 g of dry soil. High-throughput sequencing identified 207 AMF virtual taxa (VTs) from 60 soil samples, with dominant taxa, including early-evolved Paraglomus fungi and ruderal AMF, being minimally affected by soil nutrient levels. Interestingly, there was no correlation between α diversity and spore density. Structural equation modeling (SEM) indicated that host plant evolutionary divergence time (ET) directly influenced AMF α diversity indices and indirectly impacted spore density via soil nutrients. However, neither ETs nor soil nutrients significantly affected the AMF community structure, with only arbuscular abundance showing a negative correlation with ET. This research underscores the intricate relationship between host plants and AMF in genebanks, offering insights for effective AMF resource management and informed conservation practices.
Graphical Abstract
Gender patterns in engineering PhD teaching assistant evaluations corroborate role congruity theory
Authors: Evans C. A. , Adler K. , Yucalan D. , Schneider-Bentley L. M. 
Published: January 2024

Abstract
Background
The body of work regarding gender bias in academia shows that female instructors are often rated lower by students than their male counterparts. Mechanisms are complex and intersectional and often associated with role congruity theory. Little research has examined parallel patterns in graduate teaching assistant (TA) evaluations. In research institutions, TAs make up a large portion of teaching teams. Identifying bias and working to remove it is critical to shifting the already-well-documented gender imbalance in higher education. To evaluate gender-associated perceptions of graduate TAs’ teaching skills, we analyzed Likert-scale, mid-semester survey data using ordinal logistic regression models for PhD TAs in five (pre-COVID) semesters in the College of Engineering at Cornell University, a large R1 institution in the United States. We also regressed scores for each survey question against the overall TA quality rating for male- and female-identifying TAs to compare the strength of those relationships and explore potential differences in student expectations associated with gender roles. A subset of narrative comment data were coded into themes, analyzed, and triangulated with other observed patterns.

Results
Male TAs had a higher likelihood of receiving a better rating than female TAs for all survey questions in which students rated performance. Statistical evidence of different slopes of relationships between particular questions and overall TA quality rating suggested that female and male TAs were “valued” more for behaviors/attributes congruent with roles ascribed to that gender in broader society. Female TAs received a higher proportion of positive comments for communication skills and more comments regarding supportiveness than male TAs. Males received more comments about their overall value as TAs, however all comments regarding overall quality as TAs were positive regardless of gender. The amount and proportion of comments that were positive or negative for knowledge, enthusiasm, preparedness or fairness were the same for male and female TAs.

Conclusions
Gender-based disparity is occurring in TA evaluations and aligns with patterns observed in research on teaching evaluations for faculty. Correlation between overall TA ratings and scores for specific survey questions and narrative responses indicate that role congruity influences traits that students perceive as important and positive in TAs of different genders.
Andexanet alpha-induced heparin resistance treated by nafamostat mesylate in a patient undergoing total aortic arch repair for Stanford type A acute aortic dissection: a case report
Authors: Suzuki Yasuhito , Kikura Mutsuhito , Kawashima Shingo , Kimura Tetsuro , Nakajima Yoshiki 
Published: January 2024

Abstract
Background
Andexanet alfa, an anti-Xa inhibitor antagonist, induces heparin resistance. Here, we report a case of successful management of cardiopulmonary bypass with andexanet alfa-induced heparin resistance using nafamostat mesylate.

Case presentation
An 84-year-old female, with Stanford type A acute aortic dissection, underwent an emergency surgery for total aortic arch replacement. Andexanet alfa 400 mg was administered preoperatively to antagonize edoxaban, an oral Xa inhibitor. Heparin 300 IU/kg was administered before cardiopulmonary bypass, and the activated clotting time (ACT) was 291 s. The ACT was 361 s after another administration of heparin 200 IU/kg. According to our routine therapy for heparin resistance, an initial dose of nafamostat mesylate 10 mg was administered intravenously, followed by a continuous infusion of 20–30 mg/h. The ACT was prolonged to 500 s, and cardiopulmonary bypass was successfully established thereafter.

Conclusions
This case report presents the successful management of cardiopulmonary bypass with andexanet alfa-induced heparin resistance using nafamostat mesilate. This report presents the successful management of cardiopulmonary bypass with andexanet alfa-induced heparin resistance using nafamostat mesilate.
Nutritional intake, environmental factors, and their impact on myopia prevalence in Korean children aged 5–12 years
Authors: Kim Jeong-Mee , Choi Yean-Jung 
Published: January 2024

Abstract
Background
Myopia is a complex condition influenced by numerous factors, including genetic predisposition, environmental factors, and lifestyle choices. Although evidence indicates that certain dietary factors may influence the development of myopia, this relationship is still not completely understood and is a topic of ongoing research.

Methods
This study analyzed the relationship between dietary habits, environmental factors, and the prevalence of myopia in a sample of 24,345 children aged 5–12 years from the seventh Korea National Health and Nutrition Examination Survey (KNHANES VII). The average daily intake of dietary nutrients associated with the refractive error status of the participants was analyzed using analysis of variance (GLM) and the Scheffe method for post-hoc comparison. Multiple logistic regression analysis was conducted between the participant’s refractive error status and daily dietary nutrient intake, while taking into consideration the age, sex, BMI, parental myopia, and near-work hours.

Results
The risk of myopia increased with age, especially notable between ages 11 and 12, and was higher in children with both parents having myopia. Dietary factors played a crucial role; children with myopia had significantly lower intake of fat, omega-3 fatty acids, and retinol but higher intake of other nutrients compared to emmetropic and hyperopic counterparts. High consumption of carbohydrates, protein, phosphorus, iron, potassium, and sodium was associated with increased myopia risk. High sodium intake was particularly associated with a 2.05-fold increased myopia risk.

Conclusions
This study highlights the significant role of diet and lifestyle choices in the development of myopia in children. Our findings suggest the importance of considering these specific factors in the management and prevention strategies for myopia, underscoring the need for targeted interventions in children's health and vision care.
Clinical situations for which 3D printing is considered an appropriate representation or extension of data contained in a medical imaging examination: pediatric congenital heart disease conditions
Authors: Ryan Justin R. , Ghosh Reena , Sturgeon Greg , Ali Arafat , Arribas Elsa , Braden Eric , Chadalavada Seetharam , Chepelev Leonid , Decker Summer , Huang Yu-Hui , Ionita Ciprian , Lee Joonhyuk , Liacouras Peter , Parthasarathy Jayanthi , Ravi Prashanth , Sandelier Michael , Sommer Kelsey , Wake Nicole , Rybicki Frank , Ballard David 
Published: January 2024

Abstract
Background
The use of medical 3D printing (focusing on anatomical modeling) has continued to grow since the Radiological Society of North America’s (RSNA) 3D Printing Special Interest Group (3DPSIG) released its initial guideline and appropriateness rating document in 2018. The 3DPSIG formed a focused writing group to provide updated appropriateness ratings for 3D printing anatomical models across a variety of congenital heart disease. Evidence-based- (where available) and expert-consensus-driven appropriateness ratings are provided for twenty-eight congenital heart lesion categories.

Methods
A structured literature search was conducted to identify all relevant articles using 3D printing technology associated with pediatric congenital heart disease indications. Each study was vetted by the authors and strength of evidence was assessed according to published appropriateness ratings.

Results
Evidence-based recommendations for when 3D printing is appropriate are provided for pediatric congenital heart lesions. Recommendations are provided in accordance with strength of evidence of publications corresponding to each cardiac clinical scenario combined with expert opinion from members of the 3DPSIG.

Conclusions
This consensus appropriateness ratings document, created by the members of the RSNA 3DPSIG, provides a reference for clinical standards of 3D printing for pediatric congenital heart disease clinical scenarios.
Arsenic treatment of acute promyelocytic leukemia affects neutrophil function in a compensatory manner
Authors: Salzer Anna Thunström , Urban Constantin F. 
Published: January 2024

Abstract
Background
Arsenic (ATO) and retinoic acid (ATRA) are successfully used as chemotherapy-free regimens to treat acute APL. Compared to traditional chemotherapy approaches, this therapy evokes fewer haematological side effects, such as severe neutropenia and thrombocytopenia, but little is known about the impact of the treatment on neutrophil function.

Methods
We included three patients undergoing consolidation treatment for APL. To evaluate the functionality of neutrophils, we assessed chemotaxis, ROS production, and neutrophil extracellular trap (NET) release during different time points of the treatment and compared them with neutrophils from healthy donors.

Results
We revealed that the chemotactic ability of neutrophils isolated from APL patients was decreased before starting each cycle of treatment. However, there was an increase in chemotactic ability in the first week of treatment compared to other time points. Additionally, we observed increased ROS production at the start of the treatment cycle. In vitro exposure of isolated neutrophils from healthy donors to ATO led to decreased chemotaxis at high ATO concentrations exceeding those achieved in vivo, while ROS production was not affected. Chemotaxis and ROS production were not altered by exposure to ATRA in vitro and neither ATO nor ATRA had an effect on neutrophils’ ability to release NETs.

Conclusions
Our study suggests that ATO and ATRA therapy alter neutrophil function by increasing chemotaxis and reducing ROS production. The effect on neutrophil function does not, however, seem to impact infection susceptibility in our patients, indicating that the enhanced functionality might compensate for the lowered neutrophil count.
Public knowledge of risk factors and warning signs of heart attack and stroke
Authors: Pallangyo Pedro , Mkojera Zabella S. , Komba Makrina , Mfanga Loveness , Kamtoi Saad , Mmari Janeth , Faraji Husna Y. , Bhalia Smita V. , Mayala Henry A. , Matemu Genofeva , Nkinda Anna , Kifai Engerasiya , Kisenge Peter R. 
Published: January 2024

Abstract
Background
Knowledge of the predisposing risk factors and prompt recognition of the warning signs for heart attack and stroke is fundamental in modification of lifestyle behaviors and an imperative precursor to health-seeking behavior. In view of an existing knowledge gap amidst increasing incidence of heart attack and stroke in Tanzania, we conducted this community-based cross-sectional study among residents of Dar es Salaam city.

Results
A total of 1759 respondents were enrolled in this study. The mean age was 45.4 years, females constituted over a half of participants and over two-thirds had attained at least secondary school education. Regarding risk factors, just over 2% of participants displayed satisfactory awareness and only stress was recognized by at least half of participants. With regard to warning signs, barely 1% of participants had satisfactory knowledge for either of the conditions while nearly three-quarters of participants failed to mention even a single warning sign for heart attack. Recognized by about two-thirds of respondents, sudden numbness or weakness in face, arm or leg was the most acknowledged stroke symptom; however, other symptoms were familiar to less than a third of participants. Although over a half of respondents acknowledged going to a hospital as their first resort, over one-tenth of respondents expressed inappropriate reactions towards heart attack and stroke victims. Old age, higher level of education, positive history of heart attack or stroke, high blood pressure and history of dyslipidemia showed association with both risk factors and warning signs knowledge during bivariate analyses.

Conclusions
Public knowledge of common risk factors and typical warning signs for heart attack and stroke was critically suboptimal. These findings herald an utmost need for public health efforts to increase community awareness of risk factors and typical signs of the two conditions to curb the rising prevalence and associated morbimortality.
Genetic polymorphism of WNT9A is functionally associated with thumb osteoarthritis in the Chinese population
Authors: Dai Jian , Jiang Haitao , Cheng Zhang , Li Yao , Yang Zhaoqi , Cheng Chuan , Tang Xiaoming 
Published: January 2024

Abstract
Background
In a recent genome-wide association study, novel genetic variations of WNT9A were reported to be involved in the etiopathogenesis of thumb osteoarthritis (TOA) in Caucasians. Our purposes were to replicate the association of WNT9A with the development of TOA in the Chinese population and to further unveil the functional role of the risk variants.

Methods
SNP rs11588850 of WNT9A were genotyped in 953 TOA patients and 1124 healthy controls. The differences of genotype and allele distributions between the patients and healthy controls were evaluated using the Chi-square test. Luciferase Reporter Assay was performed to investigate the influence of variant on the gene expression.

Results
There was significantly lower frequency of genotype AA in TOA patients than in the controls 74.9% vs. 81.9%, p < 0.001). The frequency of allele A was remarkably lower in the patients than in the controls (86.3% vs. 90.5%, p < 0.001), with an odds ratio of 0.66 (95% CI = 0.54–0.80). Luciferase Reporter Assay showed that the construct containing mutant allele G of rs11588850 displayed 29.1% higher enhancer activity than the wild allele A construct (p < 0.05).

Conclusions
Allele G of rs11588850 was associated with the increased risk of TOA possibly via up-regulation of WNT9A expression. Further functional analysis into the regulatory role of rs11588850 in WNT9A expression can shed new light on the genetic architecture of TOA.
Genetics of biochemical attributes regulating morpho-physiology of upland cotton under high temperature conditions
Authors: Majeed Sajid , Chaudhary Muhammad Tanees , Mubarik Muhammad Salman , Rana Iqrar Ahmad , Shaban Muhammad , Tan Daniel KY , Jia Yinhua , Du Xiongming , Hinze Lori , Azhar Muhammad Tehseen 
Published: January 2024

Abstract
Background
Cotton is a strategically important fibre crop for global textile industry. It profoundly impacts several countries' industrial and agricultural sectors. Sustainable cotton production is continuously threatened by the unpredictable changes in climate, specifically high temperatures. Breeding heat-tolerant, high-yielding cotton cultivars with wide adaptability to be grown in the regions with rising temperatures is one of the primary objectives of modern cotton breeding programmes. Therefore, the main objective of the current study is to figure out the effective breeding approach to imparting heat tolerance as well as the judicious utilization of commercially significant and stress-tolerant attributes in cotton breeding. Initially, the two most notable heat-susceptible (FH-115 and NIAB Kiran) and tolerant (IUB-13 and GH-Mubarak) cotton cultivars were spotted to develop filial and backcross populations to accomplish the preceding study objectives. The heat tolerant cultivars were screened on the basis of various morphological (seed cotton yield per plant, ginning turnout percentage), physiological (pollen viability, cell membrane thermostability) and biochemical (peroxidase activity, proline content, hydrogen peroxide content) parameters.

Results
The results clearly exhibited that heat stress consequently had a detrimental impact on every studied plant trait, as revealed by the ability of crossing and their backcross populations to tolerate high temperatures. However, when considering overall yield, biochemical, and physiological traits, the IUB-13 × FH-115 cross went over particularly well at both normal and high temperature conditions. Moreover, overall seed cotton yield per plant exhibited a positive correlation with both pollen viability and antioxidant levels (POD activity and proline content).

Conclusions
Selection from segregation population and criteria involving pollen viability and antioxidant levels concluded to be an effective strategy for the screening of heat-tolerant cotton germplasms. Therefore, understanding acquired from this study can assist breeders identifying traits that should be prioritized in order to develop climate resilient cotton cultivars.