bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nature‐based strategies to regenerate the functioning and biodiversity of vineyards
Authors: Ochoa‐Hueso Raúl , Cantos‐Villar Emma , Puertas Belén , Aguiar del Rio Juan F. , Belda Ignacio , Delgado‐Baquerizo Manuel , Fernández Victoria , Gallardo Antonio , García‐Morales José L. , Garde‐Cerdán Teresa , Gonzaga‐Santesteban Luis , Lazcano Cristina , Liberal Isabel M. , Serrano‐Grijalva Lilia , Tortosa Germán , Casimiro‐Soriguer Ramón 
Published: December 2023

AbstractGrapevine is one of the most important perennial fruit crops worldwide. Historically, vineyards were compatible with soil conservation practices and multitrophic biodiversity, but vineyards are now generally eroded and biologically impoverished, making them more susceptible to pests and diseases. However, the idiosyncrasy of the wine sector places wine growers in a unique position to lead the adoption of a range of sustainable management strategies and, thus, to pioneer a wider transformation of the agricultural sector. In this article, we provide an overview of nature‐based management strategies that may be used for the regeneration of the functioning and biodiversity of vineyards and that may also lead to improved plant nutrition, grape berry quality and the suppression of pathogens and pests. These strategies include the use of microbial and nonmicrobial biostimulants, fertilization with organic amendments as well as foliar fertilization with nature‐based products, the use of cover crops and the reintegration of livestock in vineyards, especially sheep. We will also pay special attention to the implementation of circular economy in the vineyard in relation to the previously mentioned management strategies and will also discuss the importance of considering all these aspects from a holistic and integrative perspective, rather than taking them into account as single factors. Assuming the integral role of soils in the functioning of agroecosystems, soils will be considered transversally across all sections. Finally, we will argue that the time is now ripe for innovation from the public and private sectors to contribute to the sustainable management of vineyards while maintaining, or even improving, the profit margin for farmers and winemakers.
Characterization of the Starch Extracted from an Ancestral Andean Meal: Chuño
Authors: Cruz Gonzalo , Gulotta Cecilia , Bustos Ana Y. , Ribotta Pablo , Ferrero Cristina , Iturriaga Laura 
Published: December 2023

AbstractThe ancestral method of preparing chuño (freeze‐dried potato) involves three steps: freezing, soaking, and drying. The objective of this study is to identify the changes in microstructure, in physicochemical properties, and rheology of gels and suspensions of Andean potato starches caused by the procedure used to obtain chuño. A laboratory simulation of the procedure is performed using the potato variety “Rosadita” (Solanum tuberosum ssp. Andigenum). From the tests carried out on the starches extracted from the chuño obtained in the field and in the laboratory, differences phosphate content and gelatinization temperature are found. Marked differences are observed with respect to the control starch in the soluble amylose content, RVA profiles, textural attributes, and dynamic and viscous rheological properties. The chuño starches present greater resistance to granular breakdown. These observations suggest a different hydration, swelling, and gelation mechanism for the Rosadita and chuño starch samples compared to conventional potato starch and at the same time a modification due to the effect of freezing, which is more noticeable in the chuño obtained at −5 °C.
Unlocking the future of smart food packaging: biosensors, IoT, and nano materials
Authors: Bhatlawande Aboli R. , Ghatge Pravin U. , Shinde Gopal U. , Anushree R. K. , Patil Sagar D. 
Published: December 2023

AbstractThis review examines how biosensors, the Internet of Things (IoT), and nano materials can revolutionize food packaging. It highlights the limitations of traditional packaging, particularly concerning barrier properties and food quality monitoring. The paper aims to provide specific insights into the potential of these technologies. Biosensors enable real-time monitoring and spoilage detection, ensuring safer products, while IoT enhances traceability and transparency in the supply chain, leading to reduced material waste, energy waste, and operational inefficiencies, ultimately improving efficiency. Nano materials offer improved barrier capabilities, strength, and antimicrobial properties, enhancing product quality and sustainability. The review paper also discusses the promising future of smart food packaging, driven by technological advancements and consumer demand for safer and eco-friendly products. However, it acknowledges the challenges related to regulations, sustainability, and consumer acceptance that need to be addressed for widespread adoption. In conclusion, this paper demonstrates how smart food packaging with biosensors, IoT, and nano materials can transform the food industry by overcoming traditional limitations and meeting evolving consumer needs, providing improved food safety, quality, and sustainability.
dTrmt10A impacts Hsp70 chaperone m6A levels and the stress response in the Drosophila brain
Authors: Perlegos Alexandra E. , Quan Xiuming , Donnelly Kirby M. , Shen Hui , Shields Emily J. , Elashal Heidi , Fange Liu Kathy , Bonini Nancy M. 
Published: December 2023

AbstractChronic cellular stress has a profound impact on the brain, leading to degeneration and accelerated aging. Recent work has revealed the vital role of RNA modifications, and the proteins responsible for regulating them, in the stress response. In our study, we defined the role of CG14618/dTrmt10A, the Drosophila counterpart of human TRMT10A a N1-methylguanosine methyltransferase, on m6A regulation and heat stress resilience in the Drosophila brain. By m6A-IP RNA sequencing on Drosophila head tissue, we demonstrated that manipulating dTrmt10A levels indirectly regulates m6A levels on polyA + RNA. dTrmt10A exerted its influence on m6A levels on transcripts enriched for neuronal signaling and heat stress pathways, similar to the m6A methyltransferase Mettl3. Intriguingly, its impact primarily targeted 3' UTR m6A, setting it apart from the majority of Drosophila m6A-modified transcripts which display 5' UTR enrichment. Upregulation of dTrmt10A led to increased resilience to acute heat stress, decreased m6A modification on heat shock chaperones, and coincided with decreased decay of chaperone transcripts and increased translation of chaperone proteins. Overall, these findings establish a potential mechanism by which dTrmt10A regulates the acute brain stress response through m6A modification.
Semaglutide reduces tumor burden in the GAN diet-induced obese and biopsy-confirmed mouse model of NASH-HCC with advanced fibrosis
Authors: Hansen Henrik H. , Pors Susanne , Andersen Maja W. , Vyberg Mogens , Nøhr-Meldgaard Jacob , Nielsen Malte Hasle , Oró Denise , Madsen Martin Rønn , Lewinska Monika , Møllerhøj Mathias B. , Madsen Andreas Nygaard , Feigh Michael 
Published: December 2023

AbstractNon-alcoholic steatohepatitis (NASH) is emerging as a major cause of hepatocellular carcinoma (HCC), however, it is not resolved if compounds in late-stage clinical development for NASH may have additional therapeutic benefits in NASH-driven HCC (NASH-HCC). Here, we profiled monotherapy with semaglutide (glucagon-like-receptor-1 receptor agonist) and lanifibranor (pan-peroxisome proliferator-activated receptor agonist) in a diet-induced obese (DIO) mouse model of NASH-HCC. Disease progression was characterized in male C57BL/6 J mice fed the GAN (Gubra Amylin NASH) diet high in fat, fructose and cholesterol for 12–72 weeks (n = 15 per group). Other GAN DIO-NASH-HCC mice fed the GAN diet for 54 weeks and with biopsy-confirmed NASH (NAFLD Activity Score ≥ 5) and advanced fibrosis (stage F3) received vehicle (n = 16), semaglutide (30 nmol/kg, s.c., n = 15), or lanifibranor (30 mg/kg, p.o., n = 15) once daily for 14 weeks. GAN DIO-NASH-HCC mice demonstrated progressive NASH, fibrosis and HCC burden. Tumors presented with histological and molecular signatures of poor prognostic HCC. Consistent with clinical trial outcomes in NASH patients, both lanifibranor and semaglutide improved NASH while only lanifibranor reduced fibrosis in GAN DIO-NASH-HCC mice. Notably, only semaglutide reduced tumor burden in GAN DIO-NASH-HCC mice. In conclusion, the GAN DIO-NASH-HCC mouse is a clinical translational model of NASH-HCC. Semaglutide improves both NASH and tumor burden in GAN DIO-NASH-HCC mice, highlighting the suitability of this preclinical model for profiling novel drug therapies targeting NASH-HCC.
Dual barrier system against xenomitochondrial contamination in mouse embryos
Authors: Komatsu Masaya , Takuma Hikaru , Imai Shun , Yamane Maiko , Takahashi Masashi , Ikegawa Takuto , Bai Hanako , Ogawa Hidehiko , Kawahara Manabu 
Published: December 2023

AbstractHeteroplasmic mammalian embryos between genetically distant species fail to develop to term, preventing transmission of xenomitochondrial DNA to progeny. However, there is no direct evidence indicating the mechanisms by which species specificity of the mitochondrial genome is ensured during mammalian development. Here, we have uncovered a two-step strategy underlying the prevention of xenomitochondrial DNA transmission in mouse embryos harboring bovine mitochondria (mtB-M embryos). First, mtB-M embryos showed metabolic disorder by transient increase of reactive oxygen species at the 4-cell stage, resulting in repressed development. Second, trophoblasts of mtB-M embryos led to implantation failure. Therefore, we tested cell aggregation with tetraploid embryos to compensate for the placentation of mtB-M embryos. The 14 mtB-M embryos harboring bovine mtDNAs developed to term at embryonic day 19.5. Taken together, our results show that contamination of bovine mtDNA is prohibited by embryonic lethality due to metabolic disruption and failure of placentation, suggesting these represent xenomitochondrial elimination mechanisms in mammalian embryos.
Recombinant human secretory leukocyte protease inhibitor (rhSLPI) coated titanium enhanced human osteoblast adhesion and differentiation
Authors: Leelasukseree Radchanon , Chouyratchakarn Wannapat , Phutiyothin Chayanisa , Pikwong Faprathan , Srisopar Onnicha , Baipaywad Phornsawat , Udomsom Suruk , Mongkolpathumrat Podsawee , Supanchart Chayarop , Kumphune Sarawut 
Published: December 2023

AbstractOsseointegration is vital to success in orthopedic and dental reconstructions with implanted materials. The bone matrix or cells—particularly osteoblasts—are required to achieve functional contact on the implant surface. Osteoblast induction is therefore essential for osteogenesis to occur. Enhancement of osteoblast adhesion, proliferation, and differentiation, particularly by implant surface modifications, have been found challenging to develop. Secretory Leukocyte Protease Inhibitor (SLPI), a cation ionic protein with anti-inflammatory and anti-bacterial activities, showed activation in osteoblast proliferation and differentiation. However, the effects of coating recombinant human (rh) SLPI on a titanium alloy surface on human osteoblast adhesion, proliferation, and differentiation has never been investigated. In this study, titanium alloys (Ti–6Al–4V) were coated with rhSLPI, while human osteoblast adhesion, proliferation, differentiation, actin cytoskeletal organization, and gene expressions involved in cell adhesion and differentiation were investigated. The results indicate that coating titanium with 10–100 µg/ml rhSLPI enhanced the physical properties of the Ti surface and enhanced human osteoblast (hFOB 1.19) cell adhesion, activated actin dynamic, enhanced adhesive forces, upregulated integrins α1, α2, and α5, enhanced cell proliferation, mineralization, alkaline phosphatase activity, and upregulated ALP, OCN, and Runx2. This is the first study to demonstrate that coating SLPI on titanium surfaces enhances osseointegration and could be a candidate molecule for surface modification in medical implants.
Genetic variation and geographic distribution of Leishmania orientalis and Leishmania martiniquensis among Leishmania/HIV co-infection in Thailand
Authors: Ruang-areerate Toon , Ruang-areerate Panthita , Manomat Jipada , Naaglor Tawee , Piyaraj Phunlerd , Mungthin Mathirut , Leelayoova Saovanee , Siripattanapipong Suradej 
Published: December 2023

AbstractSince 1999, the number of asymptomatic leishmaniasis cases has increased continuously in Thailand, particularly among patients with HIV who are prone to develop symptoms of cutaneous and visceral leishmaniasis further. The asymptomatic infection could play a key role in Leishmania transmission and distribution. Understanding population structure and phylogeographic patterns could be crucially needed to develop effective diagnoses and appropriate guidelines for therapy. In this study, genetic variation and geographic distribution of the Leishmania/HIV co-infected population were investigated in endemic northern and southern Thailand. Interestingly, Leishmania orientalis was common and predominant in these two regions with common regional haplotype distribution but not for the others. Recent population expansion was estimated, probably due to the movement and migration of asymptomatic individuals; therefore, the transmission and prevalence of Leishmania infection could be underestimated. These findings of imbalanced population structure and phylogeographic distribution patterns provide valuable, insightful population structure and geographic distribution of Leishmania/HIV co-infection to empower prevention and control of transmission and expansion of asymptomatic leishmaniasis.
New findings on a Zintl phased K3Ag3As2 ternary semiconductor compound for photovoltaic applications by first-principles methods
Authors: Kimuyu Magdalene Mutheu , Musembi Robinson , Mwabora Julius , Nyongesa Francis 
Published: December 2023

The structural, electronic, mechanical, elastic, and optical properties of a previously unstudied Zintl-phased K3Ag3As2 ternary compound were analysed using LDA-PZ, GGA-EV, GGA-PBE, GGA-PBESol, GGA-revPBE, and SO-GGA exchange correlation functionals.
Reconfigurable ultra-sparse ventilated metamaterial absorber
Authors: Ye Yangsong , Wu Chaolin , Kang Shijie , Gao Cong , Yan Zhaoxu , Huang Yingzhou , Wu Xiaoxiao 
Published: December 2023

The impetus for examining ventilated acoustic metamaterials stems from the demand for increasingly efficient and adaptable noise reduction and sound wave manipulation techniques. However, for the present reconfigurable metamaterial absorbers, their absorption band and ventilation rate have not yet attained practical application at low frequencies (&lt;1000 Hz). A theoretical and experimental demonstration of a reconfigurable ultra-sparse ventilated metamaterial absorber for low frequencies with highly sparse ventilation and tunability is presented. The acoustic absorber is based on triadic cylindrical Helmholtz resonators, and a coupled mode theory for three resonators is implemented to elucidate the underlying physics. The absorber achieves a sparsity level of more than 80%, with a measured wind velocity ratio higher than 95%. Furthermore, tunability is accomplished in the frequency range of 600 to 950 Hz through reconfiguration. Due to its simple structure, this metamaterial absorber can be easily fabricated in mass in the future, providing promising solutions for various acoustic engineering applications.
Impact of hair-derived carbon substitution on structural and superconducting properties of MgB2
Authors: Shahbazi Mahboobeh , Pannu Amandeep Singh , Alarco Jose , Sonar Prashant , Mackinnon Ian 
Published: December 2023

This study presents a comprehensive analysis of the effects resulting from the substitution of biowaste-derived carbon-dot (CD) from human hair on structural and superconducting properties of MgB2. Syntheses of polycrystalline samples were accomplished through a standard solid-state reaction route. X-ray powder diffraction results confirm the formation of MgB2 as a primary phase in all samples and show the successful substitution of carbon for boron in MgB2. The critical current density, determined at 20 K and 4.5 T, for carbon-substituted MgB2 synthesized at 850 °C was enhanced by more than four times compared with unsubstituted MgB2. The observed improvement is due to the formation of efficient pinning centers resulting from the incorporation of carbon substituting for boron in MgB2. Furthermore, x-ray photoelectron spectroscopy (XPS) confirmed the presence of carbon bonding to boron in MgB2 synthesized with biowaste-derived CDs, indicating successful incorporation into the structure. Ultraviolet photoelectron spectroscopy (UPS) results show that the carbon-substituted MgB2 can lead to changes in the electronic band structure and values of work function. These changes significantly impact the properties of MgB2 materials, including superconducting transition temperature, upper critical field, and critical current density. The XPS and UPS experimental results are in good agreement with density functional theory calculations for MgB2 with and without carbon substitution.
The use of routinely collected electronic prescribing data to benchmark intravenous antibiotic use between two tertiary paediatric haematology-oncology inpatient units: a retrospective study
Authors: Channon-Wells Samuel , Hérin Caroline , Elbeshlawi Ismail , Gray Juliet , Patel Sanjay , Paulus Stephane 
Published: December 2023

Abstract

Background
High-quality systematic data on antimicrobial use in UK inpatient paediatric haematology-oncology services are lacking, despite this population being at high risk from antimicrobial exposure and resistance.

Objectives
We conducted a retrospective study to demonstrate how routinely collected electronic prescribing data can address this issue.

Patients and methods
This retrospective study describes and compares IV antibiotic consumption between two UK paediatric haematology-oncology inpatient units, between 2018 and 2022. Both sites provide similar services and receive proactive antimicrobial stewardship input. Data were extracted from each site’s antimicrobial surveillance system, which report monthly days of therapy (DOT) per 100 patient-days (PD). Consumption was reported for specific and total antibiotics. Trends were modelled using linear regression and autoregressive moving average models.

Results
Total IV antibiotic consumption at each site was similar. Median monthly DOT per 100 PD were 25.9 (IQR: 22.1–34.0) and 29.4 (24.2–34.9). Total antibiotic use declined at both sites, with estimated annual yearly reductions of 3.52 DOT per 100 PD (95% CI: 0.46–6.59) and 2.57 (1.30–3.85). Absolute consumption was similar for carbapenems, piperacillin/tazobactam and aminoglycosides, whilst ceftriaxone and teicoplanin demonstrated approximately 3-fold relative differences in median monthly consumption. Meropenem, piperacillin/tazobactam, teicoplanin, vancomycin and gentamicin all demonstrated statistically significant reductions in use over time at either one or both sites, although this was most marked for piperacillin/tazobactam and vancomycin.

Conclusions
Routinely collected electronic prescribing data can aid benchmarking of antibiotic use in paediatric haematology-oncology inpatients, highlighting areas to target stewardship strategies, and evaluating their impact. This approach should be rolled out nationally, and to other high-risk groups.
Susceptibility of Gram-negative isolates collected in South Korea to imipenem/relebactam and comparator agents—SMART 2018–21
Authors: Karlowsky James A , Wise Mark G , Chen Wei-Ting , Siddiqui Fakhar , Young Katherine , Motyl Mary R , Sahm Daniel F 
Published: December 2023

Abstract

Objectives
To evaluate the in vitro susceptibility of recent Gram-negative pathogens collected in South Korean medical centres to imipenem/relebactam and comparator agents.

Methods
From 2018 to 2021, six hospitals in South Korea each collected up to 250 consecutive, aerobic or facultative Gram-negative pathogens per year from patients with bloodstream, intra-abdominal, lower respiratory tract and urinary tract infections. MICs were determined using CLSI broth microdilution and interpreted by 2023 CLSI breakpoints. Most isolates that were imipenem/relebactam, imipenem or ceftolozane/tazobactam non-susceptible were screened for β-lactamase genes by PCR or WGS.

Results
Of all non-Morganellaceae Enterobacterales (NME) isolates (n = 4100), 98.8% were imipenem/relebactam susceptible. Most NME were also susceptible to imipenem alone (94.7%) and meropenem (97.3%); percent susceptible values for non-carbapenem β-lactam comparators were lower (68%–80%). Imipenem/relebactam retained activity against 96.4%, 70.8% and 70.6% of MDR, difficult-to-treat resistant (DTR) and meropenem-non-susceptible NME, respectively, and inhibited 93.1% of KPC-carrying and 95.5% of ESBL-carrying NME. Of imipenem/relebactam-resistant NME, 21/25 (84.0%) carried an MBL or an OXA-48-like carbapenemase. Of all Pseudomonas aeruginosa isolates (n = 738), 82.8% were imipenem/relebactam susceptible; percent susceptible values for all β-lactam comparators, including carbapenems (imipenem, meropenem) were 61.5%–74.7%. Less than 20% of MDR and DTR isolates, and 41% of meropenem-non-susceptible P. aeruginosa isolates were imipenem/relebactam susceptible. Of imipenem/relebactam-resistant P. aeruginosa isolates, 61.6% carried an MBL and 37.0% did not possess any acquired β-lactamase genes.

Conclusions
Based on in vitro data, imipenem/relebactam, if licensed in South Korea, may be a viable treatment option for many hospitalized patients infected with common Gram-negative pathogens including NME exhibiting MDR, DTR and carbapenem resistance and many β-lactam-resistant phenotypes of P. aeruginosa.
A Description of Exercise Programs Implemented During a Firefighter Training Academy
Authors: Mang Zachary A. , Beam Jason R. , Vigil Eric D. , Martinez Adam H. 
Published: December 2023

Objective
The aim of the study is to thoroughly describe exercise programs that were performed during a firefighter training academy, including their effects on body composition and physical fitness.

Methods
Fourteen recruits performed exercise programs during a 24-week training academy and several components of their body composition and physical fitness were measured. Moreover, their heart rate (HR) and session rating of perceived exertion (sRPE) were recorded during and after every training session, respectively.

Results
Fat-free mass, subcutaneous fat in the abdomen, cardiovascular fitness, flexibility, lower-body power, and muscular endurance significantly improved during the study (P ≤ 0.05). The highest HR and sRPE responses were observed during aerobic training (AT) sessions. The average HR responses relative to maximum HR were similar during AT, high-intensity circuit training, multimodal high-intensity interval training, and high-intensity functional training; however, the sRPEs during high-intensity circuit training and multimodal high-intensity interval training were significantly lower than AT.

Conclusions
Several effective exercise programs can be implemented during firefighter training academies.