bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing mesenchymal stem cells cultivated on microcarriers in spinner flasks via impeller design optimization for aggregated suspensions
Authors: Zhang Botao , Lu Qiaohui , Dai Gance , Zhou Yi , Ye Qian , Zhou Yan , Tan Wen-Song 
Published: December 2023

AbstractDuring the ex vivo expansion of umbilical cord-derived mesenchymal stem cells (hUCMSCs) in a stirred tank bioreactor, the formation of cell–microcarrier aggregates significantly affects cell proliferation and physiological activity, making it difficult to meet the quantity and quality requirements for in vitro research and clinical applications. In this study, computational fluid dynamic (CFD) simulations were used to investigate the effect of an impeller structure in a commercial spinner flask on flow field structure, aggregate formation, and cellular physiological activity. By designing a modified impeller, the aggregate size was reduced, which promoted cell proliferation and stemness maintenance. This study showed that increasing the stirring speed reduced the size of hUCMSC-microcarrier aggregates with the original impeller. However, it also inhibited cell proliferation, decreased activity, and led to spontaneous differentiation. Compared to low stirring speeds, high stirring speeds did not alter the radial flow characteristics and vortex distribution of the flow field, but did generate higher shear rates. The new impeller’s design changed the flow field from radial to axial. The use of the novel impeller with an increased axial pumping rate (Qz) at a similar shear rate compared to the original impeller resulted in a 43.7% reduction in aggregate size, a 37.4% increase in cell density, and a better preservation of the expression of stemness markers (SOX2, OCT4 and NANOG). Increasing the Qz was a key factor in promoting aggregate suspension and size reduction. The results of this study have significant implications for the design of reactors, the optimisation of operating parameters, and the regulation of cellular physiological activity during MSC expansion.
Graphical Abstract
Predicting cognitive dysfunction and regional hubs using Braak staging amyloid-beta biomarkers and machine learning
Authors: Bhattarai Puskar , Taha Ahmed , Soni Bhavin , Thakuri Deepa S. , Ritter Erin , Chand Ganesh B. 
Published: December 2023

AbstractMild cognitive impairment (MCI) is a transitional stage between normal aging and early Alzheimer’s disease (AD). The presence of extracellular amyloid-beta (Aβ) in Braak regions suggests a connection with cognitive dysfunction in MCI/AD. Investigating the multivariate predictive relationships between regional Aβ biomarkers and cognitive function can aid in the early detection and prevention of AD. We introduced machine learning approaches to estimate cognitive dysfunction from regional Aβ biomarkers and identify the Aβ-related dominant brain regions involved with cognitive impairment. We employed Aβ biomarkers and cognitive measurements from the same individuals to train support vector regression (SVR) and artificial neural network (ANN) models and predict cognitive performance solely based on Aβ biomarkers on the test set. To identify Aβ-related dominant brain regions involved in cognitive prediction, we built the local interpretable model-agnostic explanations (LIME) model. We found elevated Aβ in MCI compared to controls and a stronger correlation between Aβ and cognition, particularly in Braak stages III–IV and V–VII (p < 0.05) biomarkers. Both SVR and ANN, especially ANN, showed strong predictive relationships between regional Aβ biomarkers and cognitive impairment (p < 0.05). LIME integrated with ANN showed that the parahippocampal gyrus, inferior temporal gyrus, and hippocampus were the most decisive Braak regions for predicting cognitive decline. Consistent with previous findings, this new approach suggests relationships between Aβ biomarkers and cognitive impairment. The proposed analytical framework can estimate cognitive impairment from Braak staging Aβ biomarkers and delineate the dominant brain regions collectively involved in AD pathophysiology.
Bridge deck surface damage assessment using point cloud data
Authors: Al Shaini Issa , Blanco Adriana C. Trias 
Published: December 2023

AbstractBridge deck condition assessments are typically conducted through visual inspections and by incorporating traditional contact sensors for Non-Destructive Evaluation techniques such as hammer sounding and chain dragging, which require the keen expertise of trained inspectors. The accuracy of these inspections is proportional to the level of deterioration of the bridge deck, as the ability of the inspectors is correlated to the apparent level of damage. This study aims to improve the accuracy of bridge deck inspection processes by utilizing non-destructive evaluation techniques, including analyzing point cloud data gathered via Light Detection and Ranging (LiDAR) as a geometry-capturing tool for identifying surface irregularities. This research aims to evaluate and quantify the effectiveness and efficiency of LiDAR sensors in contributing to the suite of technologies available to perform bridge deck condition assessment. To achieve this, the research proposes to understand the deterioration pattern of New Jersey bridges, evaluate the results gathered from point cloud data collected on a full-scale bridge deck, and quantify the information gained from deploying LiDAR on operating bridges in New Jersey. Two data processing approaches were chosen to measure the gross and fine dimensions of the evaluated bridge decks, such as the Curvature Extraction and Slope Analysis method, and the Least Square Plane Fitting method, resulting in an accuracy of 97.92% in reference to the results gathered from reports generated through the analysis of state-of-the-art NDE technology data and visual inspection.
The “Sandwich” Extended Pedicle Subtraction Osteotomy for the Treatment of Fixed Sagittal Malalignment: Technical Description, Case Series, and Early Results With 2-Year Outcomes
Authors: Bakhsheshian Joshua , Hassan Fthimnir M. , Greisberg Gabriella , Platt Andrew , Zuckerman Scott L. , Lenke Lawrence G. 
Published: December 2023

BACKGROUND AND OBJECTIVES:
Adult spinal deformity (ASD) with fixed sagittal malalignment (FSM) may require a pedicle subtraction osteotomy (PSO) for greater focal lordosis and restoration of global alignment. Despite growing trends in minimizing PSOs given their associated high risks, a considerable portion of patients with ASD still require a lumbar PSO most commonly because of iatrogenic flat back deformity. The purpose of this article is to describe a modified extended PSO technique with additional anterior column support coined the “sandwich” extended PSO (SE-PSO) to promote arthrodesis and report the outcomes in a consecutive case series.

METHODS:
Patients with ASD treated with a lumbar SE-PSO at a single institution from 2015 to 2020 were analyzed. Complications, radiographic data, and patient-reported outcomes were compared preoperatively, at immediate postoperative follow-up, and at a 2-year postoperative follow-up (FU).

RESULTS:
Fourteen patients who underwent revision operations for FSM were included. Improvements in segmental lordosis across the PSO site (14.8 ± 6.8 vs 39.9 ± 7.1, P < .0001), overall lumbar lordosis (14.6 ± 15.4 vs 44.6 ± 12.1, P < .0001), sacral slope (21.0 ± 10.5 31.1 ± 10.7, P = .0150), C7 sagittal vertical axis (140.1 ± 59.0 mm vs 35.9 ± 28.5, P < .0001), and spinopelvic mismatch (52.5 ± 21.3 vs 18.6 ± 14.1, P = .0001) were obtained in all patients. Eight patients experienced perioperative complications, with intraoperative durotomy being the most common (n = 7). Eight patients had a 2-year FU and demonstrated improvements in their segmental lordosis across the PSO site (14.3 ± 7.0 vs 41.3 ± 7.3, P = .0003), overall lumbar lordosis (8.7 ± 17.8 vs 46.1 ± 14.2, P = .0014), sacral slope (19.1 ± 12.8 vs 32.3 ± 12.5, P = .0479), C7 sagittal vertical axis (173.6 ± 54.4 mm vs 35.8 ± 30.0, P < .0001), and spinopelvic mismatch (63.0 ± 19.7 vs 21.1 ± 18.3, P < .0001), all of which were maintained at final FU (P > .05). At 2 years, a significant increase in Scoliosis Research Society-22r total score (2.5 ± 0.8 vs 3.6 ± 0.7, P = .0023 was reported. There were no reports of symptomatic pseudarthrosis or mechanical complications.

CONCLUSION:
SE-PSO is an effective technique to correct FSM and is associated with low complications, improved patient-reported outcomes, and spinopelvic parameters that are maintained at 2 years.
Personal hygiene in individuals with a diagnosis of schizophrenia: the role of gender, clinical characteristics, and the body self.
Authors: Sakson-Obada Olga 
Published: December 2023

Cel pracyCelem badania było sprawdzenie, czy osoby z diagnozą schizofrenii różnią się w zakresie higieny osobistej (HO) od osób z grupy kontrolnej oraz ocena znaczenia płci, charakterystyk klinicznych oraz Ja cielesnego (doświadczania ciała i obrazu ciała) dla HO.MetodaPrzebadano 82 osoby z diagnozą schizofrenii w stabilnym stanie psychicznym oraz 82 osoby z grupy kontrolnej. Wszyscy badani wypełnili Skalę troski o higienę (STH), Kwestionariusz Ja cielesnego oraz odpowiedzieli na pytania Wywiadu do oceny cenestopatii. W celu potwierdzenia trafności odpowiedzi pacjentów, STH wypełniał także personel medyczny.WynikiSubiektywna ocena HO jest skorelowana z ocenami dokonywanymi przez personel medyczny. Osoby z grupy klinicznej gorzej oceniały swoją HO, niż badani z grupy kontrolnej. Mężczyźni gorzej oceniają swoją HO, niż kobiety. W przypadku chorujących mężczyzn, deficyty higieniczne szły w parze z licznymi zakłóceniami w doświadczaniu własnego ciała (cenestopatia, zakłócenia interpretacji, regulacji stanów ciała i tożsamości cielesnej) zaś w przypadku chorujących kobiet były powiązane jedynie z trudnościami w interpretacji i regulacji stanów ciała oraz negatywnymi symptomami.WnioskiOsoby z diagnozą schizofrenii w stabilnym stanie psychicznym, przejawiają deficyty w trosce o higienę, które są związane nie tyle z negatywnym ustosunkowaniem do ciała, co z zaburzonym jego doświadczaniem. Uwarunkowania deficytów higienicznych w schizofrenii są zależne od płci. W badaniu nie uczestniczyły osoby z ciężkim przebiegiem choroby, co pozwala zrozumieć brak powiązań symptomów negatywnych schizofrenii z HO w grupie badanych mężczyzn.
Assessment of knowledge and beliefs concerning mental disorders among medical students - preliminary report.
Authors: Badura Brzoza Karina , Dębski Paweł , Główczyński Patryk , Ścisło Piotr , Piegza Magdalena , Błachut Michał , Gorczyca Piotr 
Published: December 2023

Cel pracyCelem pracy była ocena postaw wobec osób z zaburzeniami psychicznymi.MetodaBadanie ankietowe przeprowadzono w grupie 93 studentów V roku studiów medycznych, kierunku lekarskiego, w tym 59 kobiet i 34 mężczyzn w wieku 24.34±1.28 lat.WynikiW przeprowadzanych badaniach osobisty kontakt z psychiatrą z powodu kłopotów z własnym zdrowiem psychicznym zadeklarowało 23% badanych. Wybór psychiatrii, jako dziedziny specjalizacji deklarowało 19% studentów. W analizie kwestionariusza dotyczącego przekonań aż 83% respondentów uznało, że choroba psychiczna jest powodem do wstydu. Analizując stosunek badanych do leczenia, wykazano, że aż 80% osób uważało, że przymusowa hospitalizacja jest słuszna. Należy podkreślić, że jedynie 31% badanych studentów uznało, że osoby z zaburzeniami psychicznymi powinny decydować się na potomstwo, a 36 % osób uważało, że osoby zaburzone psychicznie nie sprawdzą się w roli rodzica. Aż 30% badanych uważało, że osoby z zaburzeniami psychicznymi częściej popełniają przestępstwa niż osoby zdrowe, a 42% studentów wykazała brak zdecydowania w tym aspekcie.WnioskiWyniki badań sugerują konieczność poszerzania wiedzy i kształtowania właściwych postaw wobec osób z zaburzeniami psychicznymi wśród przyszły lekarzy.
Ruminations as predictors of post-traumatic stress disorder after hospitalization for Covid-19
Authors: Juczyński Zygfryd , Kwiecińska Luiza , Ogińska-Bulik Janina 
Published: December 2023

Cel pracyProblemy ze zdrowiem psychicznym należą do najczęściej zgłaszanych dolegliwości wśród osób, które były hospitalizowane z powodu Covid-19. Celem badania było ustalenie u osób hospitalizowanych z powodu Covid-19 częstości występowania PTSD oraz wyznaczenie predyktorów ryzyka jego wystąpienia.MetodaBadaniami objęto 250 osób po hospitalizacji z powodu Covid-19. Zastosowano kilka standaryzowanych narzędzi pomiaru, tj. PCL-5; PSS-10; ERRI; SPP-15 and PANAS.WynikiOgółem 55,6% badanych kwalifikowało się do diagnozy PTSD. Były to w większości osoby hospitalizowane powyżej 2 tygodni, o cięższym przebiegu choroby, badani w okresie 3 miesięcy po hospitalizacji. Do modelu regresji wprowadzono czynniki związane z przebiegiem leczenia oraz zmienne psychologiczne, zarówno zwiększające ryzyko wystąpienia PTSD, tj. spostrzegany stres, ruminowanie natrętne i emocje negatywne, jak i czynniki chroniące, tj. prężność, ruminowanie refleksyjne i emocje pozytywne. Ogólnie model regresji wyjaśnia 72% wariancji całkowitej. Najbardziej znaczące dla predykcji PTSD okazały się ruminacje natrętne, wyjaśniające 59% wariancji oraz spostrzegany stres (12%).WnioskiOsoby z rozpoznaniem PTSD wymagają pomocy terapeutycznej, dlatego tak ważne jest zidentyfikowanie czynników ryzyka, które mogą być pomocnych w usprawnieniu procesu terapii oraz ułatwieniu podejmowania działań profilaktycznych.
TÜRKLERDE GÜNEŞE SAYGI: ÇİLE GECESİ
Authors: GAZANFARGİZİ Aynur 
Published: December 2023

Çile gecesi Azerbaycan Türklerinin yaşadıkları İran’da, Türkiye’de ve Azerbaycan’da kutlnmaktadır. Dünyanın Kuzey kısmında yılın en uzun gecesi Çile gecesidir. Bu gece Şemsi yılının, Dey ayının başlangıç gecesi, Azer ayının sonuncu gecesidir.
Çile'yi soğukluk derecesine göre iki kısma bölerler: Büyük Çile, Küçük Çile. Büyük Çile 40 gün devam eder. 21 Aralık’ta başlar ve 31 Ocak’a kadar devam eder. Küçük Çile 20 gün sürer. 1 Şubat itibarile başlar ve 20 Şubat’ta biter. Çile” sözüne Divani Lügat it-Türk eserinde de rastalamaktadır. Sözün anlamı sözlükte yeşermek, başlangıc olarak verilmişdir.
Çile gecesinde nışanlı kızlara hediyeler verilir, bereket getirmesi için kalabalık sofralar kurulur. Küçük kız çocuğu kışı temsil edecek şekilde bembeyaz giydirir. Ayaz ata gelir ve hediyeler dağıtır. Çile gecesi özellikle Orta Asya’da Narduğan bayramı olarak kutlanmakdadır. Narduğan güneş doğan” demekdir.
Makalemizde bu konu arama-tarama ve kıyaslama metodları ile derinlemesine işlenecekdir. Özellikle Çile gecesi ve Narduğan bayramlarının aynı bayram olması, Türklerde ağaç kültüne saygı ve ağaçsüsleme geleneğinden de bahsedilerek eski Türk inancının izleri bayramlarda aranacaktır.
Ethnomedicinal knowledge used by Mullukurumbas of Nilgiris, Western Ghats, India
Authors: SANU C. , JEEVITH S. , SHEEBA T.C. 
Published: December 2023

Abstract. Sanu C, Jeevith S, Sheeba TC. 2023. Ethnomedicinal knowledge used by Mullukurumbas of Nilgiris, Western Ghats, India. Asian J Ethnobiol 6: 118-129. Nilgiris is one of the biodiversity-rich areas in the Western Ghats, India, with six major groups of indigenous tribes, and Mullukuruba is one of the subtribes of Kurumbas. The present study aimed to document the ethnomedicinal knowledge among the Mullukurumba Tribe of Kallical, Cherangode Village of Nilgiris, Western Ghats. Most of the tribes in Nilgiris follow a close cultural ritual and traditional knowledge, including food and medicine. The field data were collected through interviews, observation, and documentation. The tribal people utilized 152 plant species belonging to 60 families. The life forms of the collected plants revealed that herbs constitute 53 species, followed by shrubs 44 species, trees 35 species, and climbers 20 species. Family-wise, Fabaceae dominated with 13 species, followed by Asteraceae 10 species, and Solanaceae included 8 species. The use category made by the tribal community were medicinal plants (70%), edible fruits (19%), and miscellaneous uses (11%). The plant species treat various illnesses, including piles, rheumatism, gynecological issues, dermatitis, and antidiarrheal and anti-diabetic conditions. Argyreia cuneata (Willd.) Ker Gawl., Cascabela thevetia (L.) Lippold, Coix lacryma-jobi L., Ixora coccinea L., Gloriosa superba L., Melastoma malabathricum L., and Plumeria alba L. were the most common plants used for rituals. Also, this study presented an updated checklist of medicinal plants used by the Mullukurumba Tribe.
Antibacterial activities of Polyalthia longifolia leaf extracts on multiple antibiotic-resistant bacteria isolated from hospital fomites in Akure, Nigeria
Authors: BABATUNDE OLAMIDE JOSHUA , OGUNDARE AYODELE OLUYEMISI , ADEBOLU TINUOLA TOKUNBO 
Published: December 2023

Abstract. Babatunde OJ, Ogundare AO, Adebolu TT. 2023. Antibacterial activities of Polyalthia longifolia leaf extracts on multiple antibiotic-resistant bacteria isolated from hospital fomites in Akure, Nigeria. Nusantara Bioscience 15: 149-160. Polyalthia longifolia (Sonn.), an ornamental plant, is said to be therapeutic while searching for new medications to treat infections caused by multiple Antibiotic-Resistant Bacteria (MAR). This plant's leaves were investigated for prospective antibacterial activity against MAR isolated from fomites in selected hospitals in Akure and their pharmacological properties. Standard microbiological methods were used to isolate and identify bacteria from fomites. Disc diffusion was performed to test their sensitivity to conventional antibiotics and P. longifolia leaf extracts made with ethanol and water. Ciprotab® was used as the control during the antibacterial assay. Therefore, GC-MS analysis was carried out using standard methods to identify the chemicals in the plant leaf extracts. Staphylococcus aureus (29.17%), Streptococcus pyogenes (20.83%), Pseudomonas aeruginosa (14.28%), Escherichia coli (14.28%), Salmonella typhi (12.5%) and Klebsiella pneumoniae (8.33%) were the bacterial species isolated from the fomites sampled in this study. Crude ethanol leaf extract of P. longifolia (100mg/mL) inhibited the growth of these organisms with the greatest effect on P. aeruginosa with a value of 23.83±0.44 mm, which is superior to that medicated by the control antibiotic (ciprofloxacin). The GC-MS analysis of the purified leaf extracts of P. longifolia revealed the presence of bioactive compounds such as n-hexadecanoic acid and phytol, among others. The study revealed that the leaf extracts of P. longifolia can inhibit the growth of the isolated MAR from fomites with an added cidal effect, and the inhibition increase with the increase in concentration and exposure time.
Uji Aplikasi Lactobacillus lactis sebagai Paraprobiotik pada Budidaya Udang Vanamei (Litopenaeus vannamei) : Aspek Pertumbuhan Dan Imunitas
Authors: Subagiyo Subagiyo , Margino Sebastian , Triyanto Triyanto , Widada Sugeng 
Published: December 2023

Growth is one of the targets of shrimp cultivation. One factor that influences this growth is the level of immunity. Paraprobiotics are probiotics that have been inactivated, making it easier for production, storage and application. In this research, a test was carried out on the application of inactive Lactobacilus lactis to vanamei shrimp via pelleted feed (ad libitum). The experiment was carried out on a laboratory scale. There were 4 treatments, namely a single application of one of the 3 L. lactis isolates (isolate U.181, P.32 dan W-331)and a mixed of 3 L. lactis isolates. The application dose was 108 cells/gram of feed. The experiment was carried out for 35 days. Parameters observed included weight gain and cellular immune system (total number of hemocytes and hemocyte activity).The results of this study indicate that the application of the paraprobiotic L. lactis either alone or in the form of a mixture of the three can improve the growth performance and immunity of vanamei shrimp, namely being able to increase growth between 32-38% higher than the control, as well as increasing the total number of hemocytes and phagocytic activity respectively between 27.4-35.6 % and 29.1-45.9 % higher than the control. Pertumbuhan merupakan salah satu target budidaya udang. Salah satu factor yang mempengaruhi pertumbuhan adalah tingkat imunitas. Paraprobiotik adalak probiotik yang telah diinaktifkan/dimatikan, sehingga memudahkan untuk produksi, penyimpanan hingga aplikasi. Pada penelitian ini telah dilakukan uji aplikasi Lactobacilus lactis inaktif sebagai paraprobiotik ke udang vanamei melalui pakan pellet (ad libitum). Percobaa dilakukan pada skala laboratorium Ada 4 perlakuan, yaitu aplikasi tunggal salah satu dari 3 isolat L. lactis (isolat U.181, P.32 dan W-331) dan aplikasi campuran 3 isolat L. lactis. Dosis aplikasi yang dipilih adalah 108 sel/gram pakan. Percobaan dilakukan selama 35 hari. Parameter yang diamati meliputi pertambahan berat dan sistem imun seluler (total jumlah hemosit dan aktivitas hemosit). Hasil penelitian aplikasi paraprobiotik L. lactis baik secara tunggal atau dalam bentuk campuran ketiganya dapat meningkatkan performa pertumbuhan dan imunitas udang vanamei. Pada kondisi penelitian ini, apiikasi paraprobiotik mampu meningkatkan pertumbuhan antara 32-38 % lebih tinggi daripada kontrol, serta meningkatkan jumlah total hemosit dan aktivitas fagositosis berturut-turut sebesar antara 27,4-35,6 % dan 29,1-45,9 % lebih tinggi daripada kontrol.
Determination of natural and artificial radioactivity levels and radiation hazard indices for soil samples in Kırşehir
Authors: Yamçıçıer Sümeyra , Yaşar Doğan 
Published: December 2023

AbstractThe aim of this study is to create a radiological map of Kırşehir province in Turkey by analyzing the radioactivity levels of soil samples and environmental radiation measurements.226Ra,232Th,40K and137Cs radionuclides were analysed in soil samples collected from 47 locations using gamma spectrometry with an HPGe detector. According to the results obtained, gamma radioactivity concentration ranges and mean values for226Ra,232Th,40K and137Cs were determined as 133 ± 15–1515 ± 128 Bq/kg (599.40 Bq/kg), 0.3 ± 0.09–21.1 ± 1.7 Bq/kg (4.61 Bq/kg), 8.7 ± 0.9–128.5 ± 8.5 Bq/kg (29.66 Bq/kg) and 11.6 ± 8.6–273.8 ± 19.9 Bq/kg (48.80 Bq/kg) respectively. Average radioactivity levels for40K and232Th are above the world average. The mean radium equivalent activity (Raeq), gamma dose rate (D), annual effective dose equivalent (AEDE), external hazard index (Hex), and the excess lifetime cancer risk (ELCR) were calculated as 145.45 Bq/kg, 122.13 nGy/h, 0.15 mSv/h, 0.39 and 0.29 × 10−3respectively.