bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new strategy for the prevention of IgA anaphylactic transfusion reactions
Authors: Salama Abdulgabar , Temmesfeld Bettina , Hippenstiel Stefan , Kalus Ulrich , Suttorp Norbert , Kiesewetter Holger 
Published: March 2004

BACKGROUND:Management of patients with clinically significant anti‐IgA is difficult and unsatisfactory in many aspects.PATIENTS AND METHOD:A 40‐year‐old man with common variable immunodeficiency had a previous history of anaphylaxis after an intramuscular immuno‐ globulin administration. His serum contained anti‐IgA, and he required immunoglobulins for recurrent infections.RESULTS:The administration of intravenous immuno‐ globulins (IVIgG) containing less than 0.1 mg per mL IgA led to an anaphylactic reaction after the transfusion of only 2 to 3 mL. The same IVIgG charge was subsequently pretreated with freshly separated autologous plasma and given to the patient on three consecutive days without any reaction (1.25, 10, and 10 g each in 400 mL plasma). Anti‐IgA activity did not increase, and the patient was treated again without complications.DISCUSSION:Ex vivo pretreatment of IVIgG preparations with autologous plasma appears to be safe and useful in the management of patients with clinically significant anti‐IgA. To achieve a significant IgA blockage, the preparation to be used should not contain large amounts of IgA.CONCLUSION:The strategy described here appears to be safe and may help prevent anaphylaxis in many instances.
Decreased RBCTs after reduced intensity conditioning allogeneic stem cell transplantation: predictive value of prior Hb level
Authors: Ivanov V. , Faucher C. , Mohty M. , Bilger K. , Ladaique P. , Sainty D. , Arnoulet C. , Chabannon C. , Vey N. , Camerlo J. , Bouabdallah R. , Maraninchi D. , Bardou V.J. , Blaise D. 
Published: March 2004

BACKGROUND: RBCT (RBCT) requirements of stem cell transplant (SCT) recipients are often substantial and may be related to transplant type.STUDY DESIGN AND METHODS: An analysis was done of RBCT requirements and Hb recovery kinetic in the first 60 days after HLA‐identical sibling allogeneic SCT in a series of 110 consecutive patients treated for various malignant diagnoses. Patients were prepared with either an antithymocyte globulin (ATG) and reduced intensity chemotherapy‐based conditioning (RIC) (n = 64) or a myeloablative conditioning regimens (MAC; n = 46). Patients received marrow (n = 64) or PBPCs (n = 46).RESULTS: Overall, intensity of conditioning regimen (RIC vs. MAC; p = 0.0005) and graft source (PBPC vs. marrow; p < 0.0001) independently predicted RBCT requirements. Hb recovery was accelerated after RIC when compared to MAC allo‐SCT (p = 0.02). In RIC patients, RBCTs were inversely correlated to Hb level before conditioning (p < 0.0001) and the dose of ATG (p = 0.009). Moreover, Hb level before allo‐SCT significantly influenced Hb recovery kinetic after RIC but had no impact on RBCT requirements and Hb recovery after MAC.CONCLUSION: Thus, RIC conditioning creates a different pattern of erythropoiesis recovery as compared to a MAC regimen and suggest a need for studies aimed at further reducing RBCT and accelerating Hb recovery.
The effect of universal leukoreduction on postoperative infections and length of hospital stay in elective orthopedic and cardiac surgery
Authors: Llewelyn Charlotte A. , Taylor Rod S. , Todd Audrey A.M. , Stevens Warren , Murphy Mike F. , Williamson Lorna M. 
Published: March 2004

BACKGROUND: A before and after study was under‐ taken to investigate the effect of universal leukoreduction (ULR) in the UK on postoperative length of hospital stay (LOS) and infections.STUDY DESIGN AND METHODS: Consecutive patients undergoing elective coronary artery bypass grafting or total hip and/or knee replacement in 11 hospitals received non‐WBC‐reduced RBCs before implementation of ULR (T1, n = 997) or WBC‐reduced RBCs after implementation of ULR (T2, n = 1098).RESULTS: Patients in T1 and T2 were comparable except patients in T2 received on average more units of RBCs but had lower discharge Hct levels. Postoperative LOS (T1, 10 ± 8.9 days; T2, 9.6 ± 6.9 days) and the proportion of patients with suspected and proven post‐ operative infections (T1, 21.0%; T2, 20.0%) were unchanged before and after ULR (LOS, hazard ratio 1.01, 95% CI 0.92‐1.10; infections, OR 0.83, 95% CI 0.77‐1.02). Subgroup analysis showed no significant interaction between storage age or dose of blood on responsiveness of primary outcomes to ULR. Secondary outcomes were unchanged overall. Analysis by surgical procedure gave conflicting results with both increased mortality (p = 0.031) and an increased proportion of cardiac patients with proven infections (p = 0.004), whereas the proportion of orthopedic patients with proven infections was reduced (p = 0.002) after ULR.CONCLUSION: Implementation of ULR had no major impact on postoperative infection or LOS in patients undergoing elective surgical procedures who received transfusion(s). Smaller effects, either detrimental or beneficial of ULR, cannot be excluded.
Activation and removal of silicon in kaolinite by thermochemical process
Authors: Qiu Guanzhou , Jiang Tao , Li Guanghui , Fan Xiaohui , Huang Zhucheng 
Published: March 2004

The structural changes and phase transition of kaolinite during thermal treatment were investigated by thermogravimetric analysis, differential thermal analysis, infrared spectroscopy, X‐ray diffraction (XRD) and solid‐state 29Si magic angle spinning nuclear magnetic resonance in this paper. It was found that kaolinite experiences solid phase chemical transformation on heating, during which silicon in kaolinite‐layered structure is activated. The thermal treatment of kaolinite at a temperature of above 980°C results in the formation of amorphous SiO2. The recrystallization of mullite occurred when the temperature exceeded 1100°C. Quartz keeps unchanged below 1200°C.The thermochemical (TC) process provides an avenue for the removal of silicon in kaolinite. The appropriate conditions for kaolinite activation were found to be 1050–1100°C and 90–60 min. The XRD patterns of solid leached residue revealed that amorphous SiO2 could be efficiently removed by alkali leaching. The TC process was applied to desilication of kaolinite type bauxite ores. Above 50% silica total removal and 9–10 A/S ratio of desilication product were obtained at the heat conditions of 1050–1100°C and 90–60 min for an ore sample bearing 19.4% kaolinite and 4.6% quartz and with the 4.4 A/S ratio.
Including an additional systematic environmental effect within a generation in an evaluation model improves accuracy of prediction of breeding values in a closed herd of pigs
Authors: SATOH Masahiro 
Published: March 2004

ABSTRACTThe present study evaluated the advantage of mixed‐model techniques over a selection index under different magnitudes of an additional systematic environmental effect (ASEE) in terms of accuracy of prediction and expected genetic gain. The data attempted to simulate a closed herd in a pig breeding program. The base population (G0) consisted of 10 males and 50 females. Six generations (G0 to G5) were selected by using a selection index of three traits without overlapping. Additional systematic environmental constants with four levels in a generation were assigned from a uniform distribution at different ranges. Breeding values of animals in the last generation (G5) were estimated on the basis of an index of individual phenotype (SI‐U), SI‐U adjusted for ASEE using a least‐squares mean (SI‐A), best linear unbiased prediction using an animal model excluding ASEE (AM‐E), and an animal model including ASEE (AM‐I). Accuracy of prediction and expected genetic gain were larger by the animal model than by the selection index, even if heritability of the traits selected was high and ASEE was set to zero. When ASEE was zero, the accuracy of prediction and expected genetic gain given by SI‐U and AM‐I were similar to those given by SI‐A and AM‐E, respectively. However, the differences in accuracy and expected gain between SI‐U and AI‐A and between AM‐I and AM‐E increased as the range of ASEE increased. It was concluded that selection based on an animal model was more effective than index selection, even if the herd environment was uniform and traits with high heritability were selected, and that it should be always included in an evaluation model, however slight any systematic environmental effect may be in a closed herd.
Mitochondrial DNA analysis of Nepalese domestic dwarf cattle Lulu*
Authors: TAKEDA Kumiko , SATOH Masahiro , NEOPANE Shreeram P. , KUWAR Bahadur S. , JOSHI Hari D. , SHRESTHA Nanda P. , FUJISE Hiroshi , TASAI Mariko , TAGAMI Takahiro , HANADA Hirofumi 
Published: March 2004

ABSTRACTDwarf Lulu cattle, the only Bos Taurus type of cattle in Nepal, are raised under severe environments in the mountainous zone of that country. In the present study, the body measurement traits, cytogenetic and molecular genetic characteristics of the Lulu cattle are investigated. Blood samples were collected from 31 animals in four villages (altitudes 2590–3550 m) in the southern part of Mustang. The Lulu cattle had a normal karyotype with 2n = 60, XY or XX. Only one male examined had a large submetacentric X‐chromosome and a small submetacentric taurine type Y‐chromosome. The mitochodrial DNA (mtDNA) genotypes were analyzed by PCR mediated restriction fragment length polymorphisms, displacement (D)‐loop region PCR mediated single strand conformation polymorphisms, and D‐loop region sequences. Many base substitutions were found in the D‐loop region, suggesting that the Lulu cattle originated from at least 10 maternal lines. Three types of mtDNA from these cattle were found, the Bos taurus type (n = 23), the Bos indicus type (n = 6), and the Bos grunniens type (n = 2). In the village at the lowest altitude, four of the five cows were of the Bos indicus type. These results indicated that mtDNA types of the Lulu cattle mostly belong to Bos taurus, but have been hybridized with Bos indicus cattle in lower‐elevation regions in their maternal lineage.
Detection of nucleolar organizer regions on chromosomes by flourescence in situ hybridization with human 28S rRNA gene and cloning of 28S rRNA gene in Sika deer
Authors: KAGEYAMA Shuuhei , FUKUI Emiko , YOSHIZAWA Midori 
Published: March 2004

ABSTRACTThe number and loci of nucleolar organizer regions (NOR) on chromosomes in Sika deer (Cervus nippon centralis) were determined by fluorescence in situ hybridization with a human 28S ribosomal RNA (rRNA) gene as a probe. Sika deer that live in Nikko National Park and its neighboring areas (Asio and Seta) in Japan were used. All of the analyzed metaphases had three or four NOR at the end of the first and second longest telocentric autosomes. Nucleolar organizer region association, which is associated specifically on parts of NOR between chromosomes, was also observed clearly. A Sika deer 28S rRNA gene was produced by a polymerase chain reaction method. The nucleotide sequence of a Sika deer 28S rRNA gene determined by an automatic sequencer was 97 bp, and showed homogeneity of 88% for the human sequence.
Effects of cyclodextrin‐iodopropane complex on methane production, ruminal fermentation and microbes, digestibility and blood metabolites in steers
Authors: MOHAMMED Nazimuddin , LILA Zeenat Ara , TATSUOKA Noriko , HARA Koji , MIKUNI Katsuhiko , HARA Kozo , KANDA Shuhei , ITABASHI Hisao 
Published: March 2004

ABSTRACTThe effects when adding cyclodextrin‐iodopropane complex (CD‐IP) to a diet, on ruminal fermentation and microbes, digestibility, blood metabolites and methane production, were evaluated using four Holstein steers in a cross‐over design. The steers were fed Sudangrass hay plus concentrate mixture at a ratio 1.5:1, and CD‐IP (1% of dry matter) was given twice daily by mixing with concentrate mixture. Rumen and blood samples were collected at 0, 2, and 5 h after morning dosing. Ruminal pH and numbers of protozoa were unaffected by CD‐IP treatment. Ruminal molar proportion of acetate was decreased (P < 0.05), and propionate was increased (P < 0.01) at 2 h after CD‐IP dosing. Proportion of butyrate was increased (P < 0.05) and ammonia‐N was decreased (P < 0.05) at 2 and 5 h after CD‐IP dosing. Adding CD‐IP had no effect on the feed intake and digestion of nutrients. Plasma glucose was increased and urea‐N was decreased (P < 0.05) at 2 and 5 h after CD‐IP dosing. Methane production was decreased (P < 0.05) by approximately 18% in the treatment steers. Numbers of methanogenic bacteria were decreased (P < 0.05), while total viable counts, cellulolytic, sulfate reducing and acetogenic bacteria were unaffected. The present results are the first to show that CD‐IP can partially inhibit in vivo ruminal methanogenesis without adverse effects on digestion of nutrients.
Effects of concentrate supplementation on blood mineral concentration of growing upgraded Philippine goats
Authors: HAYASHIDA Maki , ORDEN Edgar A. , CRUZ Emilio M. , CRUZ Libertado C. , FUJIHARA Tsutomu 
Published: March 2004

ABSTRACTA total of 32 4‐month‐old‐upgraded Philippine goats with mean bodyweight of 13.5 kg were used to study the effects of concentrate supplementation on mineral status of growing goats. All animals received a basal diet comprised of different forage species. Twenty of the animals were supplemented with concentrate at a rate of 150–200 g dry matter/day while the remaining 12 animals were not supplemented. Blood sampling and bodyweight measurement were done every 2 weeks during the 5 months of experiment. Forage, concentrate and blood sulfur, calcium, phosphorus, magnesium, copper, zinc and selenium concentration were analyzed after wet digestion. Forage mineral contents, except for zinc and selenium, were sufficient for requirements of ruminants. Concentrate mineral contents were much higher than the requirements, but did not exceed the toxic levels. The concentrate supplementation resulted in high (P < 0.05) plasma phosphorus, copper, zinc, whole blood selenium concentrations and bodyweight of growing goats, although some supplemented animals showed plasma zinc concentration below the lower critical level. It was recommended that pellets, glass bolus or capsules that contain deficient minerals, such as zinc and selenium, should be used in the study area.
Reduction of allergic symptoms in NC/Jic Jcl mice fed a diet containing casein phosphopeptide preparation, CPP‐III
Authors: OTANI Hajime , WAKATSUKI Shidue 
Published: March 2004

ABSTRACTThe effect of a commercially available dietary casein phosphopeptide preparation, CPP‐III, consisting mainly of bovine αs2‐casein (1–32) and β‐casein (1–28) on the development of allergic symptoms mediated by IgE antibody was investigated in NC/Jic Jcl mice. No significant difference was observed in the change in mean bodyweight between mice given a diet of a mixture of ovalbumin and CPP‐III (CPP‐III‐added diet) and those given a diet of ovalbumin alone (control diet) as a protein source. Total allergic score of five mice given the CPP‐III‐added diet was less than half that of those given the control diet for 13 weeks. Levels of intestinal total and ovalbumin‐specific immunoglobulin (Ig)A were significantly higher in the CPP‐III‐added diet group than in the control diet group, while the serum ovalbumin‐specific IgE level was significantly smaller in the former. The production of interleukin (IL)‐6 by spleen cells from mice that ingested the CPP‐III‐added diet for 13 weeks was obviously higher than that by spleen cells from mice administered the control diet, while the formation of IL‐4 by spleen cells from the former mice was significantly lower than that by the latter mouse. These results suggest that the oral ingestion of CPP‐III may be beneficial in reducing allergic symptoms mediated by IgE antibody.
Immunohistological study on bovine, swine and ovine skeletal muscle fibers for the localization of fatty acid translocase FAT/CD36
Authors: BANDA Taiko , NEUPANEY Dhanapati , KIM Jin‐bo , ISHIOROSHI Makoto , MARUKAWA Katsuji , CHIBA Hitoshi , ITO Tomoo , SAMEJIMA Kunihiko 
Published: March 2004

ABSTRACTThe present immunohistological study was conducted to investigate the localization of fatty acid translocase CD36 (FAT/CD36) in the skeletal muscle (Biceps femoris) fibers of bovine, swine and ovine. The results showed that CD36 was mainly localized in type I muscle fiber of these animals. In contrast, FAT/CD36 localization in type II fiber was insignificant in the types of muscle in the present experiments, suggesting that type II fiber of bovine, swine and ovine might lack fatty acid translocase FAT/CD36.
Improvement of the gelling properties of meat emulsion gel by the addition of porcine sarcoplasmic proteins
Authors: MIYAGUCHI Yuji , HAYASHI Yuki , NAGAYAMA Kiyomi 
Published: March 2004

ABSTRACTThe effects of porcine sarcoplasmic proteins (SP) on the physicochemical properties of meat emulsion gel were examined. Meat emulsion was prepared from water‐washed pork meat (WWM), corn oil and SP. Whole SP (W‐SP) enhanced the breaking stress of the WWM emulsion gel as well as other animal proteins in the presence of 0.2 mol/L NaCl. The breaking stress of the emulsion with W‐SP increased with an increase in corn oil content. Furthermore, this tendency was more noticeable at a lower NaCl concentration (0.15 mol/L) rather than at 0.45 mol/L NaCl. Ammonium sulfate (AS) treatment fractionated W‐SP into three portions (SP‐f1, SP‐f2 and SP‐f3), which were the precipitates at 0–50% and 50–75% AS saturation and the supernatant at 75% AS saturation, respectively. The fractions SP‐f2 and SP‐f3 increased the gel strength more than W‐SP. In particular, the fraction SP‐f3 increased the gel strength approximately 10‐fold compared to the control. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis showed that SP‐f3 had several kinds of proteins and a main protein with a molecular mass of 35 kDa, which corresponded to glyceraldehyde 3‐phosphate dehydrogenase. These results indicate that the influence of SP should not be ignored when processing of low‐salt meat products and the fraction SP‐f3 has a gel‐enhancing factor for myofibrillar proteins.
Diurnal and oviposition‐related changes in heart rate, body temperature and locomotor activity of laying hens
Authors: KHALIL Ashraf M. , MATSUI Kanji , TAKEDA Ken‐ichi 
Published: March 2004

ABSTRACTDiurnal and oviposition patterns of heart rate (HR), deep body temperature (BT) and locomotor activity (LA) in conscious and unrestrained Rhode Island Red hens were studied by a radiotelemetry system. Behavioral observations were also made on diurnal changes and during the pre‐ and post‐laying period. Heart rate, BT and LA showed characteristic diurnal changes synchronized with a photoperiod of 15 h light and 9 h dark. In the light period, HR, BT, and LA levels were significantly higher than in the dark period (P < 0.001). Furthermore, the highest levels of these parameters were recorded just after they were fed (08.30 hours), while the lowest level was measured after lights‐off and remained stable throughout the dark period. Behavioral observations indicated that during the light period the hens spent most of their time in very active movement, exhibiting various behavioral patterns. However, in the dark period the hens spent almost all their time resting. The present results suggest that performing various behavioral activities cause heat generated by muscle exertion, which plays a significant role in daily HR, BT, and LA in laying hens. However, during the 60 min before and after oviposition, LA appeared to have increased steadily toward the moment of laying, and then regressed gradually in the post‐laying period to a level significantly lower than in the pre‐laying period (P < 0.001). Furthermore, the pre‐laying behavior of hens indicated extreme restlessness and more activity, whereas the post‐laying period is characterized by less activity and increased relaxation. Consequently, laying behavior has a profound but transitory effect on HR and BT, suggesting that oviposition was probably associated with intense LA.
Interaction of the New Ketolide ABT-773 (Cethromycin) with Human Polymorphonuclear Neutrophils and the Phagocytic Cell Line PLB-985 In Vitro
Authors: Labro Marie Thérèse , Abdelghaffar Houria , Babin-Chevaye Catherine 
Published: March 2004

ABSTRACTA classical velocity centrifugation technique was used to study the in vitro uptake of the new ketolide ABT-773 by human polymorphonuclear neutrophils (PMNs) and a myelomonoblastic cell line, PLB-985, which can be differentiated into PMNs under certain culture conditions, compared to that of HMR 3004. ABT-773 was rapidly taken up by PMNs (cellular concentration to extracellular concentration ratio [C/E], about 34 at 30 s and up to 207 at 5 min), and uptake plateaued from 30 to 180 min (C/E, about 300). ABT-773 was accumulated significantly better than HMR 3004 from 5 to 180 min. Nondifferentiated PLB-985 cells (ND-PLB) accumulated significantly less ABT-773 and HMR 3004 than PMNs and PLB-985 cells differentiated into PMNs (D-PLB). Whatever the cell type and in contrast to the results obtained with HMR 3004, ABT-773 was mainly located in the cytosol (about 75%) and was rapidly released from loaded cells (about 40% at 5 min), followed by a plateau, likely owing to avid reuptake. Verapamil and H89, an inhibitor of protein kinase A, increased drug efflux. Uptake was sensitive to external pH, and the activation energy was moderate (about 50 kJ/mol). The existence of an active transport system on the PMN membrane was suggested by the following findings: concentration-dependent and saturable uptake (Vmax, about 10 000 ng/2.5 × 106PMNs/5 min;Km, about 60 μg/ml) the inhibitory effects of PMN activators or inhibitors (phorbol myristate acetate, verapamil, Ni2+) and the significantly decreased levels of accumulation by killed cells and cells treated at low temperatures. In addition, various macrolides impaired ABT-773 uptake, contrary to the findings for the quinolone levofloxacin. ND- and D-PLB also presented saturation kinetics that defined an active transport system (VmaxandKmvalues were similar to those obtained with PMNs), but the activation pathway of the carrier system did not seem to be fully functional in ND-PLB. As has been observed with other erythromycin A derivatives, ABT-773 impaired oxidant production by phagocytes in a time- and concentration-dependent manner. These data extend our previous results on the existence of an active transport system common to all macrolides and ketolides, at least in PMNs.