bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histological and functional assessment of a Takotsubo cardiomyopathy model established by immobilization stress
Authors: Pei Quanwei , Yang Jiemei , Li Bin , Lin Pengqi , Zou Lina , Zhang Junpei , Yin Hongpeng , Sun Jingmei , Wang Xin , Vera A Nevzorova , Dong Zengxiang , Yin Dechun 
Published: February 2024

AbstractIntroductionTakotsubo cardiomyopathy (TTC), also known as stress‐induced cardiomyopathy, resembles acute heart failure syndrome but lacks disease‐specific diagnosis and treatment strategies. TTC accounts for approximately 5–6% of all suspected cases of acute coronary syndrome in women. At present, animal models of TTC are often created by large amounts of exogenous catecholamines such as isoproterenol. However, isoproterenol injection cannot fully simulate the onset of stress‐induced cardiomyopathy in humans since stress is not an instantaneous event.MethodsRats were immobilized for 6 h per day for 1–14 days. To examine whether the TTC model was successful, echocardiography was employed; Elisa detected serum sympathetic activation markers; and the Open‐Field test (OFT) was used to analyze behavioral changes in rats after stress. Western blot and histology were used to assess sympathetic remodeling, inflammation levels, and fibrosis; qRT‐PCR was used to explore the levels of fibrosis and myocardial hypertrophy. The electrical stability of ventricular was determined by electrophysiological testing.ResultsThe rats showed severe stress behavior and local sympathetic remodeling of the heart after only 1 day of stress. After 3 days of stress, the induction of ventricular tachyarrhythmia increased prominently. The highest incidence of TTC in rats was at 5 days of immobilization stress. The pathological left ventricular remodeling caused by immobilization (IMO) stress includes inflammatory infiltration, fibrosis, and myocardial hypertrophy.ConclusionsOur study confirms the hypothesis that IMO stress can mimic Takotsubo cardiomyopathy, and the various effects on the heart depending on the duration of IMO stress. We observed the highest incidence of TTC occurred after 5 days of stress. Furthermore, there is a gradual occurrence of electrical and structural remodeling as the stress duration prolongs.
Permanent pacemaker implantation with hybrid endocardial perventricular approach in infancy: May be an alternative route in the absence of epicardial lead
Authors: Ergul Yakup , Sahin Gulhan Tunca , Kafali Hasan Candas , Onan Ismihan Selen 
Published: February 2024

AbstractDespite the advancements in technology, establishing the optimal implantation technique for pediatric patients with a pacemaker (PM) indication remains challenging. Although the implantation of an epicardial PM is recommended, especially in children weighing less than 10 kg, transventricular placement of endocardial leads can be performed safely, offering a practical substitute for an epicardial pacing system, particularly in situations where a transvenous approach is unfeasible due to patient size, anatomical constraints or epicardial PM leads were not available as in our case.
Utility of routine follow‐up defibrillation safety margin testing in young patients with epicardial implantable cardioverter‐defibrillators
Authors: Leng Tomas , Griffeth Elaine , Stephens Elizabeth H. , Dearani Joseph A. , Cannon Bryan C. , Ackerman Michael J. , Wackel Philip L. 
Published: February 2024

AbstractBackgroundRoutine defibrillation threshold testing (DFT) of transvenous implantable defibrillators (ICDs) has largely been in decline. In patients with non‐transvenous ICDs that utilize subcutaneous and pleural ICD leads, serial DFT testing can detect a significant number of failures. Data about the utility of follow‐up defibrillation safety margin testing (DSM) testing in pediatric patients and young adults with an epicardial ICD are lacking.MethodsPatients aged < 25 years old who underwent epicardial ICD placement at Mayo Clinic from 2014 to 2023 with at least one follow‐up DSM test were included. The patients were divided into a “routine” (R) and “clinically indicated” (CI) group based on the index of clinical concern. Inadequate DSM was defined as unsuccessful defibrillation at an output of less than 10 J below the maximum output of the device. The purpose of this study was to assess the utility of follow‐up DSM testing.ResultsAn epicardial ICD system was placed in 122 patients. A total of 26 patients met inclusion criteria and underwent a total of 47 DSM follow up tests. Inadequate DSM occurred in 1/33 (3%) in the R group and 2/14 (14%) DSM tests in the CI group. The median follow‐up period was 54 and 36 months for the R and CI group, respectively.ConclusionsOur data suggest that epicardial ICDs are reliable and routine follow‐up DSM testing may not be necessary for all patients. DSM testing should be performed in individuals with epicardial ICD systems when there is clinical concern about lead or coil performance.
The Value of Cultural Similarity for Predicting Migration: Evidence from Food and Drink Interests in Digital Trace Data
Authors: Coimbra Vieira Carolina , Lohmann Sophie , Zagheni Emilio 
Published: February 2024

AbstractOne of the strongest empirical regularities in spatial demography is that flows of migrants are positively associated with population stocks at origin and destination and are inversely related to distance. This pattern was formalized into what are known as gravity models of migration. Traditionally, distance is measured geographically, but other measures of distance, such as cultural distance, are also relevant in explaining migration flows. However, measures of cultural distance are not widely adopted in the literature on modeling migration flows, partially because of the difficulties associated with operationalizing and producing these measures across space and time. In this paper, we use a scalable approach to obtain proxies for measuring cultural similarity between countries by using Facebook data and illustrate the impact of incorporating these measures, based on food and drink interests, into gravity models for predicting migration. Our results show that, despite their limitations, the new measures of cultural similarity derived from Facebook data improve the prediction power of traditional gravity models and have a predictive capacity comparable to that of classic variables used in the literature, such as shared language and history. The results open up new opportunities for understanding the determinants of migration and for predicting migration when considering broader and complementary perspectives on the meaning and measurement of distance.
Identifying Profiles of Support for Legal Abortion Services in Zambia: A Latent Class Analysis
Authors: Rosen Joseph G. , Mbizvo Michael T. , Chelwa Nachela , Phiri Lyson , Cresswell Jenny A. , Filippi Veronique , Kayeyi Nkomba 
Published: February 2024

AbstractRelative to neighboring countries, Zambia has among the most progressive abortion policies, but numerous sociopolitical constraints inhibit knowledge of pregnancy termination rights and access to safe abortion services. Multistage cluster sampling was used to randomly select 1,486 women aged 15–44 years from households in three provinces. We used latent class analysis (LCA) to partition women into discrete groups based on patterns of endorsed support for legalized abortion on six socioeconomic and health conditions. Predictors of probabilistic membership in latent profiles of support for legal abortion services were identified through mixture modeling. A three‐class solution of support patterns for legal abortion services emerged from LCA: (1) legal abortion opponents (∼58 percent) opposed legal abortion across scenarios; (2) legal abortion advocates (∼23 percent) universally endorsed legal protections for abortion care; and (3) conditional supporters of legal abortion (∼19 percent) only supported legal abortion in circumstances where the pregnancy threatened the fetus or mother. Advocates and Conditional supporters reported higher exposure to family planning messages compared to opponents. Relative to opponents, advocates were more educated, and Conditional supporters were wealthier. Findings reveal that attitudes towards abortion in Zambia are not monolithic, but women with access to financial/social assets exhibited more receptive attitudes towards legal abortion.
Phosphatidylcholine‐deficient suppressor mutant of Sinorhizobium meliloti, altered in fatty acid synthesis, partially recovers nodulation ability in symbiosis with alfalfa (Medicago sativa)
Authors: García‐Ledesma Juan Daniel , Cárdenas‐Torres Luis , Martínez‐Aguilar Lourdes , Chávez‐Martínez Ana I. , Lozano Luis , López‐Lara Isabel M. , Geiger Otto 
Published: February 2024

SUMMARYRhizobial phosphatidylcholine (PC) is thought to be a critical phospholipid for the symbiotic relationship between rhizobia and legume host plants. A PC‐deficient mutant of Sinorhizobium meliloti overproduces succinoglycan, is unable to swim, and lacks the ability to form nodules on alfalfa (Medicago sativa) host roots. Suppressor mutants had been obtained which did not overproduce succinoglycan and regained the ability to swim. Previously, we showed that point mutations leading to altered ExoS proteins can reverse the succinoglycan and swimming phenotypes of a PC‐deficient mutant. Here, we report that other point mutations leading to altered ExoS, ChvI, FabA, or RpoH1 proteins also revert the succinoglycan and swimming phenotypes of PC‐deficient mutants. Notably, the suppressor mutants also restore the ability to form nodule organs on alfalfa roots. However, nodules generated by these suppressor mutants express only low levels of an early nodulin, do not induce leghemoglobin transcript accumulation, thus remain white, and are unable to fix nitrogen. Among these suppressor mutants, we detected a reduced function mutant of the 3‐hydoxydecanoyl‐acyl carrier protein dehydratase FabA that produces reduced amounts of unsaturated and increased amounts of shorter chain fatty acids. This alteration of fatty acid composition probably affects lipid packing thereby partially compensating for the previous loss of PC and contributing to the restoration of membrane homeostasis.
The Ca2+‐MdCRF4‐MdWRKY9 module negatively affects apple fruit watercore formation by suppressing the transcription of MdSOT2
Authors: Li Hongjian , Si Yajing , Wang Hong , Liu Zhi , Lu Qian , Liu Li , Li Tong , Wang Aide 
Published: February 2024

SUMMARYWatercore is a common physiological disease of Rosaceae plants, such as apples (Malus domestica), usually occurring during fruit ripening. Apple fruit with watercore symptoms is prone to browning and rotting, thus losing commercial viability. Sorbitol and calcium ions are considered key factors affecting watercore occurrence in apples. However, the mechanism by which they affect the occurrence of watercore remains unclear. Here, we identified that the transcription factor MdWRKY9 directly binds to the promoter of MdSOT2, positively regulates the transcription of MdSOT2, increases sorbitol content in fruit, and promotes watercore occurrence. Additionally, MdCRF4 can directly bind to MdWRKY9 and MdSOT2 promoters, positively regulating their expression. Since calcium ions can induce the ubiquitination and degradation of the transcription factor MdCRF4, they can inhibit the transcription of MdWRKY9 and MdSOT2 by degrading MdCRF4, thereby reducing the sorbitol content in fruit and inhibiting the occurrence of fruit watercore disease. Our data sheds light on how calcium ions mitigate watercore in fruit, providing molecular‐level insights to enhance fruit quality artificially.
Deciphering PDH1's role in mung bean domestication: a genomic perspective on pod dehiscence
Authors: Li Shuai , Li Yaling , Zhu Hong , Chen Liyang , Zhang Huiying , Lian Lijie , Xu Miaomiao , Feng Xilong , Hou Rui , Yao Xiaolin , Lin Yifan , Wang Huaying , Wang Xutong 
Published: February 2024

SUMMARYMung bean (Vigna radiata) stands as a crucial legume crop in Asia, contributing to food security. However, our understanding of the underlying genetic foundation governing domesticated agronomic traits, especially those linked to pod architecture, remains largely unexplored. In this study, we delved into the genomic divergence between wild and domesticated mung bean varieties, leveraging germplasm obtained from diverse sources. Our findings unveiled pronounced variation in promoter regions (35%) between the two mung bean subpopulations, suggesting substantial changes in gene expression patterns during domestication. Leveraging transcriptome analysis using distinct reproductive stage pods and subpopulations, we identified candidate genes responsible for pod and seed architecture development, along with Genome‐Wide Association Studies (GWAS) and Quantitative Trait Locus (QTL) analysis. Notably, our research conclusively confirmed PDH1 as a parallel domesticated gene governing pod dehiscence in legumes. This study imparts valuable insights into the genetic underpinnings of domesticated agronomic traits in mung bean, and simultaneously highlighting the parallel domestication of pivotal traits within the realm of legume crops.
Successful treatment pure red cell aplasia after ABO major mismatched allogeneic hematopoietic stem cell transplantation with avatrombopag and low dose rituximab
Authors: Cao Junjie , Lu Shaoyan , Luo Danjie , Pei Renzhi , Lu Ying , Chen Dong , Du Xiaohong , Li Shuangyue 
Published: February 2024

AbstractBackgroundPure red cell aplasia (PRCA) following allogeneic hematopoietic stem cell transplantation (allo‐HSCT) with ABO major incompatibility is characterized by transfusion dependent anemia. No standard treatment existed for PRCA following allo‐HSCT yet.Study Design and MethodsWe conducted a retrospective study, and reported our experience with the use of avatrombopag and lower dose rituximab to treat five patients with PRCA subsequent to major ABO‐incompatible allo‐HSCT.ResultsFive cases of PRCA were identified from 72 patients who underwent allo‐HSCT with major or bidirectional ABO mismatch. Cumulative incidence at Day +60 was 6.9% (5/72) at our center. All donor and recipient blood groups were A+ and O+, respectively. In the first three cases we reported, patients received erythropoietin, plasma exchange, and donor lymphocyte infusion, but none of them had any effect. After 4 weeks of treatment with low dose rituximab (100 mg/week) combined with avatrombopag (40 mg/day), favorable outcomes were obtained. According to the aforementioned experience, Cases 4 and 5 were administered low‐dose rituximab and avatrombopag in 3 months after transplantation, and erythroid response was observed on 3 weeks after treatment. Our patients tolerated low‐dose rituximab and avatrombopag well and experienced rapid efficacy, with a median duration of 3 weeks. Furthermore, no severe infection or thrombocytosis necessitated a dose adjustment.ConclusionLow‐dose rituximab and avatrombopag may be an effective treatment for patients with PRCA after major ABO‐incompatible allo‐HSCT. The patients should be treated at least 90 days post transplantation if conventional erythropoietin therapy fails.
Keloids in male genitalia—Systematic review and illustrative case report
Authors: Isoldi Felipe Contoli , Garcia Ariane , Vieites Luiza , Ferreira Lydia Masako 
Published: February 2024

AbstractKeloid is the maximum expression of pathological fibroproliferative skin wound healing, whose pathophysiology is not yet fully understood. Its occurrence in the perineum and genitalia is uncommon. A systematic review was carried out regarding the occurrence and treatment of keloids on the penis. An illustrative case was also reported. The review used the PRISMA checklist and was registered in PROSPERO. The entire literature period up to April 2023 was searched in the EMBASE/Elsevier, Cochrane, Scopus, Medline, BVS, SciELO, and Lilacs databases. The inclusion criteria embraced primary studies, clinical trials, prospective or retrospective cohorts, case series, case–control studies and case reports. Three hundred and sixty‐one studies were found and 12 of them were included, consisting of 9 case reports and 3 case series. The most common triggering factor for keloid formation was circumcision, in 11 of the cases, of which more than half occurred in prepubescent children. Several therapies, associated or isolated, were used to treat the cases. Only one of the reported patients had scar recurrence after surgical treatment. Studies with better scientific evidence are needed to understand the involvement of keloids in male genitalia. However, keloid formation in this topography is rare, making it difficult to carry out more elaborate studies.
Diophantine approximation, large intersections and geodesics in negative curvature
Authors: Ghosh Anish , Nandi Debanjan 
Published: February 2024

AbstractIn this paper, we prove quantitative results about geodesic approximations to submanifolds in negatively curved spaces. Among the main tools is a new and general Jarník–Besicovitch type theorem in Diophantine approximation. Our framework allows manifolds of variable negative curvature, a variety of geometric targets, and logarithm laws as well as spiraling phenomena in both measure and dimension aspect. Several of the results are new also for manifolds of constant negative sectional curvature. We further establish a large intersection property of Falconer in this context.
SKIPping With PAX: Evaluating the Effects of a Dual-Component Intervention on Gross Motor Skill and Social–Emotional Development
Authors: Brian Ali , Munn Emily E. , Abrams T. Cade , Case Layne , Taunton Miedema Sally , Stribing Alexandra , Lee Unjong , Griffin Stephen 
Published: February 2024

Improving the development of the social, emotional, and physical domains during early childhood impacts the overall trajectory of a child’s well-being. However, researchers often address these independently, leaving a gap for a more integrated approach to promoting development. This study explores the effects of a dual-component intervention on changes in preschool-aged boys’ and girls’ gross motor and social–emotional skills. Preschoolers (N = 475; girls = 220 and boys = 255) ages 3–6 years completed the 9-month dual-component intervention and were randomized into control (n = 148) or intervention (n = 327) groups by classroom. Significant improvements were observed in social skills, locomotor, and total Test of Gross Motor Development-3. Additionally, boys and girls improved at the same rate in ball skills, locomotor, and total Test of Gross Motor Development-3. These results suggest that the dual-component intervention can improve preschoolers’ social skills and motor skills with no differential effects.
Complete genome sequence of
Luteolibacter
sp. strain LG18, an
L
-glucose-utilizing bacterium isolated from soil
Authors: Yachida Masashi , Nakamura Akira 
Published: February 2024

ABSTRACT

An L-glucose-utilizing bacterium,
Luteolibacter
sp. strain LG18, was isolated from soil, and the complete genome sequence was determined. Strain LG18 contained a single circular chromosome of 5.80 Mb with a G + C content of 64.5%, in which 4,598 protein-coding genes, 9 rRNA, and 56 tRNA genes were identified.
MicroMPN: methods and software for high-throughput screening of microbe suppression in mixed populations
Authors: Franco Meléndez Karla , Schuster Layla , Donahey Melinda Chue , Kairalla Emily , Jansen M. Andrew , Reisch Christopher , Rivers Adam R. 
Published: February 2024

ABSTRACT

Screening assays are used to test if one or more microbes suppress a pathogen of interest. In the presence of more than one microbe, the screening method must be able to accurately distinguish viable pathogen cells from non-viable and non-target microbes in a sample. Current screening methods are time-consuming and require special reagents to detect viability in mixed microbial communities. Screening assays performed using soil or other complex matrices present additional challenges for screening. Here, we develop an experimental workflow based on the most probable number (MPN) assay for testing the ability of synthetic microbial communities to suppress a soil-borne pathogen. Our approach, fluorMPN, uses a fluorescently labeled pathogen and microplate format to enable high-throughput comparative screening. In parallel, we developed a command-line tool, MicroMPN, which significantly reduces the complexity of calculating MPN values from microplates. We compared the performance of the fluorMPN assay with spotting on agar and found that both methods produced strongly correlated counts of equal precision. The suppressive effect of synthetic communities on the pathogen was equally recoverable by both methods. The application of this workflow for discriminating which communities lead to pathogen reduction helps narrow down candidates for additional characterization. Together, the resources offered here are meant to facilitate and simplify the application of MPN-based assays for comparative screening projects.

IMPORTANCE
We created a unified set of software and laboratory protocols for screening microbe libraries to assess the suppression of a pathogen in a mixed microbial community. Existing methods of fluorescent labeling were combined with the most probable number (MPN) assay in a microplate format to enumerate the reduction of a pathogenic soil microbe from complex soil matrices. This work provides a fluorescent expression vector available from Addgene, step-by-step laboratory protocols hosted by protocols.io, and MicroMPN, a command-line software for processing plate reader outputs. MicroMPN simplifies MPN estimation from 96- and 384-well microplates. The microplate screening assay is amenable to robotic automation with standard liquid handling robots, further reducing the hands-on processing time. This tool was designed to evaluate synthetic microbial communities for use as microbial inoculates or probiotics. The fluorMPN method is also useful for screening chemical and antimicrobial libraries for pathogen suppression in complex bacterial communities like soil.
Chan–Lam N-Arylation of Adamantane-Containing Amines
Authors: Kuliukhina D. S. , Malysheva A. S. , Averin A. D. , Savelyev E. N. , Orlinson B. S. , Novakov I. A. , Beletskaya I. P. 
Published: February 2024

Abstract
The Chan–Lam reaction conditions were optimized for the synthesis of N-aryl derivatives of adamantane-containing amines. A number of adamantane-containing amines and diamines with different steric hindrances at the primary amino groups were reacted with p-tolylboronic acid under the optimized conditions [0.1 M solution of amine in MeCN, p-tolylboronic acid (2 equiv), DBU (2 equiv), copper(II) acetate (20 mol %), 25°C, 24 h]. The reactivity of the amines was found to strongly depend on their structure, and the maximum yield of the target products reached 74% from the monoamines and 66% from the diamines.