bluuebunny/crossref_metadata_2025_split huggingface dataset:
Az emberi tényező szerepe a vadászturisztikai szolgáltatások minőségmenedzsmentjében
Authors: Magyar Zoltán 
Published: January 2024

A „vadászat” mára markáns turisztikai motivációnak is tekinthető, és egyértelművé vált a „vadászati turizmus” ország-és desztinációimázs alakulásában játszott szerepe. Az Országos Magyar Vadászkamara beszámolója alapján a 2021/2022-as vadászati évben 29 506 fő külföldi vadászvendég járt hazánkban, az ágazat a tavalyi vadászati évet 3780 millió Ft (+11,48%) egyenleggel zárta. Robinson & Novelli (2005) megfogalmazása szerint a vadászati turizmus olyan „niche” turizmusnak is tekinthető, ami egyfajta autentikus környezetben megvalósuló, speciális érdeklődési kör által motivált turisztikai forma, ennek megfelelően a fogyasztóknak csak egy szűkebb rétegét érinti. A „vadászati turizmus” esetében az ügyfelek élménye az ügyfél és a szolgáltató közötti – gyakran személyre szabott – interakción keresztül, a közös élményteremtés folyamatán keresztül jön létre. A szolgáltató a megfelelő élménykörnyezetet kialakításával és a vadállomány, mint limitáló termelési tényező biztosításától kezdőden, majd a szolgáltatásfolyamat során egyfajta asszisztenciaként segíti elő az élményt jelentő eseményt. A szolgáltató és az ügyfél között lezajló interakció minősége nagyban befolyásolja egyrészt a vadászat sikerességét, másrészt annak élményjellegét is. A vadásztató és az ügyfél ilyen formájú eredményes „együttműködése” sokkal erősebb bizalmi viszonyt feltételez és tesz szükségessé, mint az a turizmus többi típusában (pl. a tömegturizmus egyes típusai) jellemző. A tanulmány célja, hogy a fókuszcsoportos vizsgálatok és az esettanulmányok alapján feltárja az vadászturisztikai szolgáltatások teljesítésében részt vevő „emberi tényező” ügyfelek által észlelt és szolgálatatás-minőség alakulásában játszott szerepét, és meghatározza azokat a karakterjellemzőket, melyek alapvetők és kardinálisak az ügyfelek elégedettsége, visszatérési szándéka szempontjából. A vizsgálatok eredményei beszámolnak arról, hogy a vadászturisztikai szolgáltatásokat igénybe vevő ügyfelek számára az élményfolyamatban részt vevő, velük kontaktusba kerülő szolgáltató, mint „emberi tényező” egyértelműen meghatározó és sajátos szerepet játszik a szolgáltatás élménytartalmának és az ügyfelek elégedettségének alakulásában. Ez a meghatározó szerep az azonosított karakterjellemzőkön keresztül nyilvánul meg, melyek direkt módon hatnak a vadászturisztikai termékek egyes attribútumaira is.
Egyetemi hallgatók és vállalkozás
Authors: S. Gubik Andrea , Farkas Szilveszter 
Published: January 2024

Az egyetemi hallgatók között egyre inkább növekszik azoknak az aránya, akik saját vállalkozásuk megalapításán gondolkoznak, vagy már aktív vállalkozók. A felsőoktatási intézmények a hagyományos oktatási feladataikon túl egyre aktívabb szerepet vállalnak a vállalkozásösztönzésben különféle programok, erőforrások rendelkezésre bocsátásával, és azzal is, hogy megpróbálják megérteni, hogy milyen mechanizmusok állnak a hallgatói döntések mögött, milyen irányú változtatások tennék lehetővé a trendek kedvező irányba történő elmozdulását. Ezt a célt szolgálja a Global University Entrepreneurial Spirit Students' Survey (GUESSS) nemzetközi kutatási együttműködés is, amelynek keretében 50 ország fogott össze a hallgatói vállalkozási kérdések kutatása terén. Tanulmányunk célja a magyarországi helyzet áttekintő bemutatása, amelyhez a 2021-es adatfelvétel eredményeit használjuk fel. 2021-ben a kérdőívet minden olyan magyarországi felsőoktatási intézménynek elküldtük, amely több mint 1000 hallgatónak nyújt képzést, és 20 intézményből 10 104 érvényes választ kaptunk. A felmérés eredményei szerint a hallgatók többsége (74,4%) a diploma megszerzése után munkavállalóként szeretne dolgozni, öt évvel később azonban jelentősen nő a vállalkozói hajlandóság. A közgazdasági és üzleti képzések hallgatói a legnyitottabbak a saját vállalkozás indítására, míg a társadalomtudományi képzésekben részt vevők számára a legkevésbé vonzó a vállalkozói életpálya. Ugyan a családi háttér nagyon erős befolyásoló tényező az onnan érkező társadalmi támogatás (erőforrások, tudás, tapasztalat) miatt, de a vállalkozásoktatás és általában az egyetemek vállalkozói környezete is ösztönzőleg hat a hallgatók elképzeléseire. A Covid-19 a vállalkozói elképzeléseket sem hagyta érintetlenül, a vállalkozásindításon dolgozó hallgatók 6,7 százaléka, a már vállalkozással rendelkező hallgatók 12,9 százaléka válaszolta azt, hogy a vállalkozásának elindításában a Covid-19 jelentős szerepet játszott.
Case Study Analysis of the Project of Educational Material Development for SME Joint Ventures in V4 Countries to Strengthen SMEs Through Supporting Innovation in Education
Authors: Kozák Tamás , Gajzágó Éva , Soós Gabriella 
Published: January 2024

“Innovate or die!” is an often-heard statement indicating the urgent need for companies to engage in innovation to survive and grow in a sustainable way (Vanhaverbeke, 2017). The main objective of the project is to develop the education at theme of small and medium entrepreneurs (SMEs) in Visegrad countries (V4). The expected results are strengthening the abilities and competencies of students to launch start-up businesses in V4 regions, stimulating cross-border entrepreneurship cooperation in the V4 region, and building up new business networks for SMEs, start-ups, and research institutions within and outside V4. The primary objective of the project is strengthening the joint mutual representation of the V4 economies through educational development. Based on the tradition of important relations between the members in several fields of economy, the cooperation should be extended with learning of other countries’ economic, social, and cultural dimensions. Participating students will be able to launch new SME businesses within V4. The objective project goal is to strengthen the cooperation among the participating universities’ students in the field of profound understanding of different cultural, technological, and regional economic policies. In terms of coordinated and integrated educational programs, the projects strengthen professional networks of partner universities and support the mobility of students, researchers, and educators to share best practices in the education of business management. The common training and research program includes themes, such as SME-s in general, the profile of the SME sector, main Characteristics of the SME Sector, SME-s in V4, managing SMS-s, understanding environment, macro-economy, regulation, SME finance, strategy, analysis of competencies, strategic planning, project management, operational planning of SME, inventory management, selling activity for SME’s, human resources, performance measurement, staff management and legal environments to launch new business.
Influence of medium modifications (optimization) on high nematicidal activity of the fermentation broth of Clostridium beijerinckii
Authors: Liu Shuang , Zhang Kejun , Yu Yun , Lian Xinglong , Jiang Lanyuwen , Meng Fanqi , Wang Yuanyuan , Zhu Xiaofeng , Duan Yuxi 
Published: January 2024

Introduction: The nematode species Meloidogyne incognita has been responsible for significant financial losses within the agricultural sector. Nematophagous bacteria, characterised by their extensive distribution and broad spectrum of hosts, exhibit remarkable efficacy as natural antagonists against nematodes. Sneb518 (Clostridium beijerinckii) fermentation broth displayed substantial biocontrol activity against M. incognita in previous research. Optimizing fermentation conditions is a fundamental technique for dramatically enhancing end product performance. There has been no such study conducted yet on enhancing the nematicidal activities of Sneb518 (Clostridium beijerinckii) fermentation using response surface methodology (RSM).Methods: The influence of strain Sneb518 fermentation media and conditions on nematicidal activity was examined using the three-factor technique and a Plackett-Burman design, and the interaction between various fermentation factors was examined using a Box-Behnken design. The present study employed response surface methodology (RSM) to examine and enhance the nematicidal activity of Sneb518 culture filtrates by identifying and optimising the influential components.Results: Glucose, peanut cake flour, and potassium chloride as carbon, nitrogen, and inorganic salts displayed considerably increased nematicidal potential in the present study. Furthermore, the corrected mortality of J2 ranged from 52.24% to 91.15% when utilizing the Box-Behnken design. These findings clearly support the application of RSM for medium optimization. Moreover, the outcomes of the validation experiment corresponded to the model predictions.Discussion: This research has enhanced the biocontrol ability of C. beijerinckii to control M. incognita and this research has led to the advancement of new biocontrol agents.
Prediction of stroke patients’ bedroom-stay duration: machine-learning approach using wearable sensor data
Authors: Ogasawara Takayuki , Mukaino Masahiko , Matsunaga Kenichi , Wada Yoshitaka , Suzuki Takuya , Aoshima Yasushi , Furuzawa Shotaro , Kono Yuji , Saitoh Eiichi , Yamaguchi Masumi , Otaka Yohei , Tsukada Shingo 
Published: January 2024

Background:The importance of being physically active and avoiding staying in bed has been recognized in stroke rehabilitation. However, studies have pointed out that stroke patients admitted to rehabilitation units often spend most of their day immobile and inactive, with limited opportunities for activity outside their bedrooms. To address this issue, it is necessary to record the duration of stroke patients staying in their bedrooms, but it is impractical for medical providers to do this manually during their daily work of providing care. Although an automated approach using wearable devices and access points is more practical, implementing these access points into medical facilities is costly. However, when combined with machine learning, predicting the duration of stroke patients staying in their bedrooms is possible with reduced cost. We assessed using machine learning to estimate bedroom-stay duration using activity data recorded with wearable devices.Method:We recruited 99 stroke hemiparesis inpatients and conducted 343 measurements. Data on electrocardiograms and chest acceleration were measured using a wearable device, and the location name of the access point that detected the signal of the device was recorded. We first investigated the correlation between bedroom-stay duration measured from the access point as the objective variable and activity data measured with a wearable device and demographic information as explanatory variables. To evaluate the duration predictability, we then compared machine-learning models commonly used in medical studies.Results:We conducted 228 measurements that surpassed a 90% data-acquisition rate using Bluetooth Low Energy. Among the explanatory variables, the period spent reclining and sitting/standing were correlated with bedroom-stay duration (Spearman’s rank correlation coefficient (R) of 0.56 and −0.52,p&lt; 0.001). Interestingly, the sum of the motor and cognitive categories of the functional independence measure, clinical indicators of the abilities of stroke patients, lacked correlation. The correlation between the actual bedroom-stay duration and predicted one using machine-learning models resulted in an R of 0.72 andp&lt; 0.001, suggesting the possibility of predicting bedroom-stay duration from activity data and demographics.Conclusion:Wearable devices, coupled with machine learning, can predict the duration of patients staying in their bedrooms. Once trained, the machine-learning model can predict without continuously tracking the actual location, enabling more cost-effective and privacy-centric future measurements.
Antioxidant and anticholinesterase properties of Echinometra mathaei and Ophiocoma erinaceus venoms from the Persian Gulf
Authors: Dehghani Hamideh , Rashedinia Marzieh , Mohebbi Gholamhossein , Vazirizadeh Amir , Baghban Neda 
Published: January 2024

Introduction: The Persian Gulf is home to a diverse range of marine life, including various species of fish, crustaceans, mollusks, and echinoderms. This study investigates the potential therapeutic properties of venoms from echinoderms in the Persian Gulf, specifically their ability to inhibit cholinesterases (Acetylcholinesterase and butyrylcholinesterase) and act as antioxidants.Methods: Four venoms from two echinoderm species, including the spine, gonad, and coelomic fluids of sea urchins, as well as brittle star venoms, were analyzed using various methods, including LD50 determination, protein analysis, antioxidant assays, GC-MS for secondary metabolite identification, and molecular docking simulations.Results and discussion: The study’s results revealed the LD50 of the samples as follows: 2.231 ± 0.09, 1.03 ± 0.05, 1.12 ± 0.13, and 6.04 ± 0.13 mg/mL, respectively. Additionally, the protein levels were 44.037 ± 0.002, 74.223 ± 0.025, 469.97 ± 0.02, and 104.407 ± 0.025 μg/mL, respectively. SDS-PAGE and total protein studies indicated that at least part of the venom was proteinaceous. Furthermore, the study found that the brittle star samples exhibited significantly higher antioxidant activity compared to other samples, including the standard ascorbic acid, at all tested concentrations. GC-MS analysis identified 12, 23, 21, and 25 compounds in the samples, respectively. These compounds had distinct chemical and bioactive structures, including alkaloids, terpenes, and steroids.Conclusion: These venoms displayed strong cholinesterase inhibitory and antioxidant activities, likely attributed to their protein content and the presence of alkaloids, terpenes, and steroids. Notably, the alkaloid compound C7 was identified as a promising candidate for further research in Alzheimer’s disease therapy. In conclusion, echinoderms in the Persian Gulf may hold significant potential for discovering novel therapeutic agents.
Clopidogrel, ticagrelor, prasugrel or an alternation of two P2Y12 in patients with acute myocardial infarction with cardiogenic shock
Authors: Kanic Vojko , Kompara Gregor 
Published: January 2024

BackgroundData are lacking on the effects of the alternation of P2Y12 receptor antagonists (P2Y12) on bleeding and outcome in patients with myocardial infarction (MI) with cardiogenic shock (CS). We compared the effects of different P2Y12 and alternation of P2Y12 (combination) on bleeding and outcome in patients with MI and CS.MethodsData from 247 patients divided into four groups: clopidogrel, ticagrelor, prasugrel, and the combination group, were analyzed. The association between P2Y12 and bleeding as well as 30-day and one-year mortality was examined.ResultsThe highest bleeding rate was observed in patients in the combination group, followed by the clopidogrel, ticagrelor, and prasugrel groups [12(50%) patients, 22(28.2%), 21(18.3%) and 4(13.3%), respectively;p = 0.003]. Bleeding occurred with a similar frequency in the combination and clopidogrel groups (p = 0.081), but more frequently than in the ticagrelor and prasugrel groups (p = 0.002 andp = 0.006, respectively). Bleeding rates were similar in patients receiving P2Y12 alone (p = 0.13). Compared to clopidogrel, both ticagrelor and prasugrel had a lower bleeding risk (aOR: 0.40; 95% CI: 0.18–0.92;p = 0.032 and aOR: 0.20; 95% CI: 0.05–0.85;p = 0.029, respectively) and the combination had a similar bleeding risk (aOR: 2.31; 95% CI: 0.71–7.48;p = 0.16). The ticagrelor and prasugrel groups had more than an 80% and 90% lower bleeding risk than the combination group (aOR: 0.17; 95% CI: 0.06–0.55;p = 0.003 and aOR: 0.09; 95% CI: 0.02–0.44;p = 0.003, respectively). The unadjusted 30-day and one-year mortality were highest in the clopidogrel group, followed by the ticagrelor, prasugrel, and combination groups (44(56.4%) and 55(70.5%) patients died in the clopidogrel group, 53(46.1%) and 56(48.7%) in the ticagrelor group, 12(40%) and 14(46.7%) patients died in the prasugrel, and 6(25%) and 9(37.5%) patients died in the combination group;p = 0.045 andp &lt; 0.0001. After adjustment for confounders, the P2Y12 groups were not independently associated with either 30-day (p = 0.23) or one-year (p = 0.17) mortality risk.ConclusionOur results suggest that the choice of P2Y12 was not associated with treatment outcome. The combination of P2Y12 increased bleeding risk compared with ticagrelor and prasugrel and was comparable to clopidogrel in patients with MI and CS. However, these higher bleeding rates did not result in worse treatment outcomes.
Integrated analysis of single-cell RNA-seq and chipset data unravels PANoptosis-related genes in sepsis
Authors: Dai Wei , Zheng Ping , Wu Jian , Chen Siqi , Deng Mingtao , Tong Xiangqian , Liu Fen , Shang Xiuling , Qian Kejian 
Published: January 2024

BackgroundThe poor prognosis of sepsis warrants the investigation of biomarkers for predicting the outcome. Several studies have indicated that PANoptosis exerts a critical role in tumor initiation and development. Nevertheless, the role of PANoptosis in sepsis has not been fully elucidated.MethodsWe obtained Sepsis samples and scRNA-seq data from the GEO database. PANoptosis-related genes were subjected to consensus clustering and functional enrichment analysis, followed by identification of differentially expressed genes and calculation of the PANoptosis score. A PANoptosis-based prognostic model was developed. In vitro experiments were performed to verify distinct PANoptosis-related genes. An external scRNA-seq dataset was used to verify cellular localization.ResultsUnsupervised clustering analysis using 16 PANoptosis-related genes identified three subtypes of sepsis. Kaplan-Meier analysis showed significant differences in patient survival among the subtypes, with different immune infiltration levels. Differential analysis of the subtypes identified 48 DEGs. Boruta algorithm PCA analysis identified 16 DEGs as PANoptosis-related signature genes. We developed PANscore based on these signature genes, which can distinguish different PANoptosis and clinical characteristics and may serve as a potential biomarker. Single-cell sequencing analysis identified six cell types, with high PANscore clustering relatively in B cells, and low PANscore in CD16+ and CD14+ monocytes and Megakaryocyte progenitors. ZBP1, XAF1, IFI44L, SOCS1, and PARP14 were relatively higher in cells with high PANscore.ConclusionWe developed a machine learning based Boruta algorithm for profiling PANoptosis related subgroups with in predicting survival and clinical features in the sepsis.
Chemical immune conization of precancerous cervical lesions awakens immune cells and restores normal HPV negative and abnormal proliferation
Authors: Liu Xueping , Yu Baofa , Gao Feng , Jing Peng , Zhang Peicheng , Zheng Guoqin , Zhang Xiaomin 
Published: January 2024

BackgroundCervical cancer is one of the most common and deadly cancers in women, which is closely linked to the persistent infection of high-risk human papillomavirus (HPV). Current treatment of cervical cancer involves radical hysterectomy, radiotherapy, and chemotherapy or a combination.ObjectiveWe investigated if hapten-enhanced intratumoral chemotherapy (HEIC) was effective in boosting immunity for effective treatment of precancerous cervical lesions and HPV infection.Study designWe used single-cell RNA sequencing (scRNA-Seq) to obtain transcriptome profiles of 40,239 cells from biopsies of precancerous cervical lesions from the cervix directly from one patient before the start of HEIC and approximately 1 week after HEIC. The blood samples were taken at the same time as biopsies. We compared the expression characteristics of malignant epithelial cells and immune cells, including epithelial cells, endothelial cells (ECs), fibroblasts, mural cells, T cells, B cells, T and NK neutrophils, mast cells, microparticles (MPs), and platelets, as well as the dynamic changes in cell percentage and cell subtype heterogeneity.ResultsIntratumoral injection of chemotherapy drug plus hapten induces an acute immune response in precancerous cervical lesions with HPV and further awakens immune cells to prevent the abnormal proliferation of the precancerous cells.ConclusionHEIC provides a potential treatment method for cervical cancer and HPV infection tailored to each patient’s condition.
Refined innate plasma signature after rVSVΔG-ZEBOV-GP immunization is shared among adult cohorts in Europe and North America
Authors: Martinez-Murillo Paola Andrea , Huttner Angela , Lemeille Sylvain , Medaglini Donata , Ottenhoff Tom H. M. , Harandi Ali M. , Didierlaurent Arnaud M. , Siegrist Claire-Anne 
Published: January 2024

BackgroundDuring the last decade Ebola virus has caused several outbreaks in Africa. The recombinant vesicular stomatitis virus-vectored Zaire Ebola (rVSVΔG-ZEBOV-GP) vaccine has proved safe and immunogenic but is reactogenic. We previously identified the first innate plasma signature response after vaccination in Geneva as composed of five monocyte-related biomarkers peaking at day 1 post-immunization that correlates with adverse events, biological outcomes (haematological changes and viremia) and antibody titers. In this follow-up study, we sought to identify additional biomarkers in the same Geneva cohort and validate those identified markers in a US cohort.MethodsAdditional biomarkers were identified using multiplexed protein biomarker platform O-link and confirmed by Luminex. Principal component analysis (PCA) evaluated if these markers could explain a higher variability of the vaccine response (and thereby refined the initial signature). Multivariable and linear regression models evaluated the correlations of the main components with adverse events, biological outcomes, and antibody titers. External validation of the refined signature was conducted in a second cohort of US vaccinees (n=142).ResultsEleven additional biomarkers peaked at day 1 post-immunization: MCP2, MCP3, MCP4, CXCL10, OSM, CX3CL1, MCSF, CXCL11, TRAIL, RANKL and IL15. PCA analysis retained three principal components (PC) that accounted for 79% of the vaccine response variability. PC1 and PC2 were very robust and had different biomarkers that contributed to their variability. PC1 better discriminated different doses, better defined the risk of fever and myalgia, while PC2 better defined the risk of headache. We also found new biomarkers that correlated with reactogenicity, including transient arthritis (MCP-2, CXCL10, CXCL11, CX3CL1, MCSF, IL-15, OSM). Several innate biomarkers are associated with antibody levels one and six months after vaccination. Refined PC1 correlated strongly in both data sets (Geneva: r = 0.97, P &lt; 0.001; US: r = 0.99, P&lt; 0.001).ConclusionEleven additional biomarkers refined the previously found 5-biomarker Geneva signature. The refined signature better discriminated between different doses, was strongly associated with the risk of adverse events and with antibody responses and was validated in a separate cohort.