bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display
Authors: Szöllősi Dávid , Hajdrik Polett , Tordai Hedvig , Horváth Ildikó , Veres Dániel S. , Gillich Bernadett , Shailaja Kanni Das , Smeller László , Bergmann Ralf , Bachmann Michael , Mihály Judith , Gaál Anikó , Jezsó Bálint , Barátki Balázs , Kövesdi Dorottya , Bősze Szilvia , Szabó Ildikó , Felföldi Tamás , Oszwald Erzsébet , Padmanabhan Parasuraman , Gulyás Balázs Zoltán , Hamdani Nazha , Máthé Domokos , Varga Zoltán , Szigeti Krisztián 
Published: October 2023

AbstractThe important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain (E. coli BL21(DE3) ΔnlpI, ΔlpxM) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs.
Seasonal variations in the phenolic profile, antioxidant activity, and mineral content of south Indian black tea (Camellia sinensis (L.) O. Kuntze)
Authors: Govindasamy Kottur , Sugumar Daisylin Anbu Sujitha , Kandan N. Mani , Nagaprasad N. , Ramaswamy Krishnaraj 
Published: October 2023

AbstractIn the Anamallais region of south India, crop shoots from the UPASI-3, UPASI-9, UPASI-17, Assam seedlings, and TRI-2043 cultivars were examined for seasonal variations in total phenolics, antioxidant activity, and minerals during four harvest seasons: summer (January to March), premonsoon (April and May), monsoon (June to September), and winter (October to December) of two consecutive years. The total phenolics of all cultivars were lower in monsoon period and grew over rest of the seasons and it was greater during summer. Crop shoot antioxidant activity as measured by the DPPH radical scavenging experiment exhibited a similar pattern to total phenolics. Summer was the season with the highest antioxidant activity across all cultivars, followed by premonsoon, winter, and monsoon. On the other hand, the employed cultivars differed noticeably in terms of seasonal change of minerals. These results appear to indicate that the harvest period is hypercritical in deciding the antioxidant potency of tea crop shoots.
Individual and population diversity of 20 representative olfactory receptor genes in pigs
Authors: Kang Mingue , Ahn Byeongyong , Youk Seungyeon , Jeon Hyoim , Soundarajan Nagasundarapandian , Cho Eun-Seok , Park Woncheoul , Park Chankyu 
Published: October 2023

AbstractUnderstanding the influence of genetic variations in olfactory receptor (OR) genes on the olfaction-influenced phenotypes such as behaviors, reproduction, and feeding is important in animal biology. However, our understanding of the complexity of the OR subgenome is limited. In this study, we analyzed 1120 typing results of 20 representative OR genes belonging to 13 OR families on 14 pig chromosomes from 56 individuals belonging to seven different breeds using a sequence-based OR typing method. We showed that the presence of copy number variations, conservation of locus-specific diversity, abundance of breed-specific alleles, presence of a loss-of-function allele, and low-level purifying selection in pig OR genes could be common characteristics of OR genes in mammals. The observed nucleotide sequence diversity of pig ORs was higher than that of dogs. To the best of our knowledge, this is the first report on the individual- or population-level characterization of a large number of OR family genes in livestock species.
Deciphering morphological and biochemical responses of Salvia leriifolia to seed cold plasma treatment, priming, and foliar spraying with nano-salicylic acid
Authors: Ghodsimaab Seyedeh Parisa , Ghasimi Hagh Ziba , Makarian Hassan , Gholipoor Manoochehr 
Published: October 2023

AbstractThe pretreatment of seeds with cold plasma (CP) (0 and 100 w for 240 s), and salicylic acid priming (SA) (0 and 2 mM normal and nano form), and foliar spraying of SA at the six-leaf stage (0 and 2 mM normal and nano form) ofSalvia leriifoliaplants in field condition was studied. Compared to the control plants ofS. leriifolia, the results showed that CP + both forms of SA priming + nano-SA spraying increased plant height, leaf length, plant dry weight, total phenol, and the activities of phenylalanine ammonia-lyase (PAL) and tyrosine ammonia-lyase (TAL) enzymes. The chlorophyll a and b contents in all treated plants remained either unchanged or decreased when compared to the control. The highest PAL activity was obtained in CP-free + hydro-priming + nano-SA foliar spraying. The highest content of caffeic acid was achieved in CP + SA priming + SA foliar spraying in the leaf. The maximum contents of rosmarinic and salvianolic acid were obtained in the control plants. In conclusion, CP and nano-SA can increase PAL and TAL activity and total phenol accumulation inS. leriifoliaplants, but not rosmarinic and salvianolic acid contents. Other phenolic compound enzymes and their production require further study.
Facile fabrication of carboxymethylcellulose/ZnO/g-C3N4 containing nutmeg extract with photocatalytic performance for infected wound healing
Authors: Youshi Maysa , Farahpour Mohammad Reza , Tabatabaei Zohreh Ghazi 
Published: October 2023

AbstractNew topical antibacterial agents are required to inhibit and development of bacteria and also promoting the wound healing process. This study was evaluating the healing effect of Myristica fragrans extract coated with carboxymethyl cellulose, zinc oxide and graphite carbon nitride (CMC/ZnO/g-C3N4/MyR) by photocatalytic process on the healing process of full-thickness infectious excision wounds in mice. Nanosheets were prepared and physicochemical properties were evaluated. Safety, in vitro release, antibacterial activities under in vitro and in vivo condition, wound contraction, histopathological properties and the protein expressions of tumor necrosis factor-α (TNF-α), collagen 1A (COL1A) and CD31 were also evaluated. Physicochemical properties confirmed their successful synthesis. Nanosheets exhibited antibacterial activity under in vitro and in vivo conditions. The formulations containing CMC/ZnO/g-C3N4/MyR, significantly (P < 0.05) competed with standard ointment of mupirocin for accelerating the wound healing process due to their effects on bacterial count and the expression of TNF-α and also accelerating the proliferative phase. This structure can be used as a safe structure in combination with other agents for accelerating the wound healing process following future clinical studies.
Network efficiency of spatial systems with fractal morphology: a geometric graphs approach
Authors: Flores-Ortega A. C. , Nicolás-Carlock J. R. , Carrillo-Estrada J. L. 
Published: October 2023

AbstractThe functional features of spatial networks depend upon a non-trivial relationship between the topological and physical structure. Here, we explore that relationship for spatial networks with radial symmetry and disordered fractal morphology. Under a geometric graphs approach, we quantify the effectiveness of the exchange of information in the system from center to perimeter and over the entire network structure. We mainly consider two paradigmatic models of disordered fractal formation, the Ballistic Aggregation and Diffusion-Limited Aggregation models, and complementary, the Viscek and Hexaflake fractals, and Kagome and Hexagonal lattices. First, we show that complex tree morphologies provide important advantages over regular configurations, such as an invariant structural cost for different fractal dimensions. Furthermore, although these systems are known to be scale-free in space, they have bounded degree distributions for different values of an euclidean connectivity parameter and, therefore, do not represent ordinary scale-free networks. Finally, compared to regular structures, fractal trees are fragile and overall inefficient as expected, however, we show that this efficiency can become similar to that of a robust hexagonal lattice, at a similar cost, by just considering a very short euclidean connectivity beyond first neighbors.
Acoustic compression in Zoom audio does not compromise voice recognition performance
Authors: Perepelytsia Valeriia , Dellwo Volker 
Published: October 2023

AbstractHuman voice recognition over telephone channels typically yields lower accuracy when compared to audio recorded in a studio environment with higher quality. Here, we investigated the extent to which audio in video conferencing, subject to various lossy compression mechanisms, affects human voice recognition performance. Voice recognition performance was tested in an old–new recognition task under three audio conditions (telephone, Zoom, studio) across all matched (familiarization and test with same audio condition) and mismatched combinations (familiarization and test with different audio conditions). Participants were familiarized with female voices presented in either studio-quality (N = 22), Zoom-quality (N = 21), or telephone-quality (N = 20) stimuli. Subsequently, all listeners performed an identical voice recognition test containing a balanced stimulus set from all three conditions. Results revealed that voice recognition performance (dʹ) in Zoom audio was not significantly different to studio audio but both in Zoom and studio audio listeners performed significantly better compared to telephone audio. This suggests that signal processing of the speech codec used by Zoom provides equally relevant information in terms of voice recognition compared to studio audio. Interestingly, listeners familiarized with voices via Zoom audio showed a trend towards a better recognition performance in the test (p = 0.056) compared to listeners familiarized with studio audio. We discuss future directions according to which a possible advantage of Zoom audio for voice recognition might be related to some of the speech coding mechanisms used by Zoom.
Effect of cyanide-utilizing bacteria and sulfur on feed utilization, microbiomes, and cyanide degradation in cattle supplemented with fresh cassava root
Authors: Sombuddee Napudsawun , Prachumchai Rittikeard , Khota Waroon , Boontiam Waewaree , Cherdthong Anusorn 
Published: October 2023

AbstractThis study aimed to compare the effects of adding cyanide-utilizing bacteria (CUB) and sulfur on rumen fermentation, the degradation efficiency of hydrogen cyanide (HCN), feed utilization, and blood metabolites in beef cattle fed two levels of fresh cassava root (CR). A 2 × 2 factorial arrangement in a 4 × 4 Latin square design was used to distribute four male purebred Thai native beef cattle (2.5–3.0 years old) with an initial body weight (BW) of 235 ± 15.0 kg. Factor A wasEnterococcus faeciumKKU-BF7 oral direct fed at 108 CFU/ml and 3% dry matter (DM) basis of pure sulfur in concentrate diet. Factor B was the two levels of CR containing HCN at 300 and 600 mg/kg on DM basis. There was no interaction effect between CUB and sulfur supplementation with CR on feed utilization (p > 0.05). Similarly, CUB and sulfur supplementation did not affect (p > 0.05) DM intake and apparent nutrient digestibility. However, the high level of CR supplementation increased (p < 0.05) feed intake and neutral detergent fiber digestibility. The ruminal pH, microbial population, ammonia–nitrogen, blood urea nitrogen, and blood thiocyanate concentrations were unaffected by the addition of CUB and sulfur at two CR concentrations (p > 0.05). The addition of CUB or sulfur had no effect on the efficiency of HCN degradation in the rumen (p > 0.05). However, cattle given CR with HCN at 600 mg/kg DM had considerably higher degradation efficiency than those fed CR containing HCN at 300 mg/kg DM (p < 0.05). The group fed CUB had a considerably greater CUB population (p < 0.05) than the sulfur group. Cyanide-utilizing bacteria or sulfur supplementation with CR had no interaction effect between total VFAs and their profiles (p > 0.05). However, the study observed a significant positive correlation between the amount of CR and the concentration of propionate in the rumen (p < 0.05). The levels of nitrogen absorption and nitrogen retention did not differ significantly among the treatments (p > 0.05). Hence, it may be inferred that the administration of a high concentration of CR at a dosage of 600 mg/kg DM HCN could potentially provide advantageous outcomes when animals are subjected to oral CUB incorporation.
The impact and complete genome characterisation of viruses involved in outbreaks of gastroenteritis in a farrow-to-finish holding
Authors: Brnić Dragan , Vlahović Dunja , Gudan Kurilj Andrea , Maltar-Strmečki Nadica , Lojkić Ivana , Kunić Valentina , Jemeršić Lorena , Bačani Ivica , Kompes Gordan , Beck Relja , Mikuletič Tina , Steyer Andrej 
Published: October 2023

AbstractViral enteric pathogens continuously burden intensive pig farming, causing gastrointestinal diseases of epidemic and endemic nature. The present study investigated two diarrhoea outbreaks on a large farrow-to-finish holding and subsequent circulation of outbreak-related enteric viruses. These viruses were characterised by whole genome sequencing, and statistical evaluation of the impact on specific production metrics was performed. The results provided evidence that the Porcine epidemic diarrhoea virus–swine enteric coronavirus (PEDV–SeCoV) S gene recombinant strain was responsible for the first outbreak, whilst Rotavirus A (RVA) in a mixed infection with Rotavirus B (RVB) and porcine kobuvirus (PKV) probably caused the second diarrhoea outbreak. Whole genome characterisation revealed a porcine origin of all viruses involved and significant heterogeneity of RVB strain, proposing four novel genotypes and changes in RVB VP1 genotype classification. The statistical evaluation confirmed only a minor disturbance in the number of weaned pigs per sow, with statistical forecasting showing positive trends. A follow-up study corroborated the endemicity of RVA and PKV, in contrast to PEDV–SeCoV. Punctual, comprehensive and timely investigation of diarrhoea outbreaks is a prerequisite for applying adequate pig health and biosecurity management. Calculating such outbreaks' impact on production metrics can potentially shape future decisions on management improvements.
Association between depression and endometriosis using data from NHANES 2005–2006
Authors: Hu Pan-Wei , Zhang Xiao-Le , Yan Xiao-Tong , Qi Cong , Jiang Guo-Jing 
Published: October 2023

AbstractStudies on the association between depression and self-reported endometriosis are limited, and further studies are required to investigate this association. Data were collected from the National Health and Nutrition Examination Survey database (2005–2006). Based on the inclusion and exclusion criteria, 100 participants with self-reported endometriosis and 1295 participants without self-reported endometriosis were included, representing a total population of 64,989,430. Depression severity was assessed using the Patient Health Questionnaire 9 (PHQ9). A survey-weighted logistic regression analysis was performed to explore the association between depression and endometriosis. Subgroup analyses were conducted to explore heterogeneity. The prevalence of endometriosis was 7.17%. A significant positive association was found between the PHQ9 score and endometriosis. After adjusting for all covariates, the PHQ9 score positively correlated with endometriosis. Furthermore, compared with the participants without depression, those with moderate depression were more prone to have endometriosis both in unadjusted and fully adjusted model. However, the relationship between severe depression and endometriosis was not significant in all models (P > 0.05). Our findings highlight the influence of depression on the prevalence of self-reported endometriosis. Further studies are required to elucidate the causal relationship between depression and self-reported endometriosis.
Crystals structures of carbonate phases with Mg in Triassic rocks, mineral formation and transitions
Authors: Stanienda-Pilecki Katarzyna J. 
Published: October 2023

AbstractDetailed data on carbonate minerals different in magnesium content including their crystal structures were presented in this article. The samples were collected from the formations of the Lower Muschelkalk and the Upper Muschelkalk. X-ray diffraction and electron microprobe analysis were used for carbonate phases determination. The following minerals with different Mg content were identified: low-Mg calcite, high-Mg calcite, proto-dolomite, ordered dolomite and de-huntite. The content of Mg in high-Mg calcite is higher than in low-Mg calcite but lower than that of proto-dolomite. Proto-dolomite is characterized by lower content of Mg than typical for stoichiometric value for dolomite—ordered dolomite. Due to the reduced Mg content in identified huntite, this carbonate phase was named as de-huntite. The research results are significant because they are a source of new data on carbonate phases with different Mg content which build studied Triassic rocks and crystal structures of these minerals.
Analysis of risk factors and prognostic factors of brain metastasis in gastric cancer: a surveillance, epidemiology and end-results database study
Authors: Rehman Mohammad Ebad Ur , Kulsoom Afifa , Faraz Fatima , Mustafa Biah , Shahid Abia , Cheema Huzaifa Ahmad , Maqbool Shahzaib , Khan Israr , Hussain Taimoor , Iftikhar Ahmed , Awan Rehmat Ullah , Swed Sarya , Raza Shahzad , Anwer Faiz 
Published: October 2023

AbstractBrain metastasis in gastric cancer (GC) patients is a rare phenomenon that is associated with adverse clinical outcomes and poor survival rates. We conducted a retrospective cohort study to investigate the incidence, risk factors and prognostic factors of brain metastasis in GC patients. Data on sociodemographic and tumor characteristics of GC patients from 2010 to 2019 was extracted from the Surveillance, Epidemiology and End-Results (SEER) database. Descriptive statistics, multivariable logistic and Cox regression were applied on SPSS. Kaplan–Meier-Survival curves and ROC curves were constructed. A total of 59,231 GC patients, aged 66.65 ± 13.410 years were included. Brain metastasis was reported in 368 (0.62%) patients. On logistic regression, the risk of brain metastasis was significantly greater in males, patients aged < 60 years and patients having concurrent bone and lung metastasis. High grade and high N stage were significant risk factors for development of brain metastasis. Patients who had undergone surgery for the primary tumor were at reduced risk for brain metastasis (adjusted odds ratio 0.210, 95% CI 0.131–0.337). The median OS was 3 months in patients with brain metastasis and 17 months in patients without brain metastasis (p < 0.05). On Cox regression, Grade IV tumors and primary antral tumors were significant predictable parameters for poor prognosis. Overall Survival (OS) and Cancer-Specific Survival (CSS) were prolonged in patients who had undergone surgery. Brain metastasis in gastric cancer is associated with significantly worse survival. Employing large-scale screening for high-risk patients holds a promising impact to improve survival rates, but it must be accurately balanced with a comprehensive understanding of clinicopathological aspects for accurate diagnosis and treatment.
Evidence of a metacognitive illusion in judgments about the effects of music on cognitive performance
Authors: Bell Raoul , Komar Gesa Fee , Mieth Laura , Buchner Axel 
Published: October 2023

AbstractTwo experiments serve to examine how people make metacognitive judgments about the effects of task-irrelevant sounds on cognitive performance. According to the direct-access account, people have direct access to the processes causing auditory distraction. According to the processing-fluency account, people rely on the feeling of processing fluency to make heuristic metacognitive judgments about the distracting effects of sounds. To manipulate the processing fluency of simple piano melodies and segments of Mozart’s sonata K. 448, the audio files of the music were either left in their original forward direction or reversed. The results favor the processing-fluency account over the direct-access account: Even though, objectively, forward and backward music had the same distracting effect on serial recall, stimulus-specific prospective metacognitive judgments showed that participants incorrectly predicted only backward music but not forward music to be distracting. The difference between forward and backward music was reduced but not eliminated in global retrospective metacognitive judgments that participants provided after having experienced the distracting effect of the music first-hand. The results thus provide evidence of a metacognitive illusion in people’s judgments about the effects of music on cognitive performance.
Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager
Authors: Zhdanov Alexander V. , Sen Rajannya , Devoy Ciaran , Li Liang , Tangney Mark , Papkovsky Dmitri B. 
Published: October 2023

AbstractMonitoring of tissue O2 is essential for cancer development and treatment, as hypoxic tumour regions develop resistance to radio- and chemotherapy. We describe a minimally invasive technique for the monitoring of tissue oxygenation in developing grafted tumours, which uses the new phosphorescence lifetime based Tpx3Cam imager. CT26 cells stained with a near-infrared emitting nanoparticulate O2 probe NanO2-IR were injected into mice to produce grafted tumours with characteristic phosphorescence. The tumours were allowed to develop for 3, 7, 10 and 17 days, with O2 imaging experiments performed on live and euthanised animals at different time points. Despite a marked trend towards decreased O2 in dead animals, their tumour areas produced phosphorescence lifetime values between 44 and 47 µs, which corresponded to hypoxic tissue with 5–20 μM O2. After the O2 imaging in animals, confocal Phosphorescence Lifetime Imaging Microscopy was conducted to examine the distribution of NanO2-IR probe in the tumours, which were excised, fixed and sliced for the purpose. The probe remained visible as bright and discrete ‘islands’ embedded in the tumour tissue until day 17 of tumour growth. Overall, this O2 macro-imaging method using NanO2-IR holds promise for long-term studies with grafted tumours in live animal models, providing quantitative 2D mapping of tissue O2.