bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reach of the Instagram profile @microbioworld in popularizing mycology and microbiology
Authors: dos Reis Jefferson Brendon Almeida , Schirmer Sofia Coradini 
Published: February 2025

Abstract
Online social networks have revolutionized scientific communication, making platforms like Instagram indispensable for sharing complex topics, including mycology. This study evaluated three key factors in assessing the impact of social media on scientific dissemination: follower profiles, reach, and engagement levels. We used the professional Instagram account @microbioworld as a case study. Account performance data were collected over a 90-day period (12 August–9 November 2024). Post performance was evaluated using data from selected posts published between 11 January and 11 November 2024. By the end of our sampling period, the account reached a total of 45 959 followers, with the majority aged 25–34 years (44.8%). It reached 108 631 unique accounts, with 22.4% being followers and 77.6% non-followers, generating 236 860 impressions and 15 750 interactions. Likes accounted for 83.3% of engagement. Posts featuring microorganism cultures achieved the highest engagement and reach. Sentiment analysis, using Bing and AFINN lexicons, revealed that over 89% of sentiments expressed in comments were positive. These findings demonstrate how Instagram can disseminate microbial content, foster positive perceptions of microorganisms, and emphasize their ecological importance, encouraging audience involvement with mycology and microbiology.
Comprehensive survey of kombucha microbial communities of diverse origins and fermentation practices
Authors: Ben Saad Emna , Friedrich Anne , Fischer Frédérique , Courot Olivier , Schacherer Joseph , Bleykasten Claudine 
Published: February 2025

Abstract
Kombucha is a unique, naturally fermented sweetened tea produced for thousands of years, relying on a symbiotic microbiota in a floating biofilm, used for successive fermentations. The microbial communities consist of yeast and bacteria species, distributed across two phases: the liquid and the biofilm fractions. In the fermentation of kombucha, various starters of different shapes and origins are used, and there are multiple brewing practices. By metabarcoding, we explored here the consortia and their evolution from a collection of 23 starters coming from various origins summarizing the diversity of kombucha fermentation processes. A core microbiota of yeast and bacteria has been identified in these diverse kombucha symbiotic consortia, revealing consistent core taxa across symbiotic consortium of bacteria and yeasts from different starters. The common core consists of five taxa: two yeast species from the Brettanomyces genus (B. bruxellensis and B. anomalus) and bacterial taxa Komagataeibacter, Lactobacillus, and Acetobacteraceae, including the Acetobacter genus. The distribution of yeast and bacteria core taxa differs between the liquid and biofilm fractions, as well as between the “mother” and “daughter” biofilms used in successive fermentations. In terms of microbial composition, the diversity is relatively low, with only a few accessory taxa identified. Overall, our study provides a deeper understanding of the core and accessory taxa involved in kombucha fermentation.
Two Concepts of Liberty in Paradise Lost
Authors: Woodford Benjamin 
Published: February 2025

Abstract
John Milton’s Paradise Lost portrays a conflict between two types of freedom, one espoused by God, the other by Satan. These two freedoms reflect Isaiah Berlin’s definitions of positive and negative liberty. God leaves the angels and Adam and Eve free to choose without interference (negative freedom), while Satan links freedom to self-mastery (positive freedom). An all-powerful, creator God and Satan’s dictatorial leadership result in dependence being a crucial aspect of both versions of freedom; consequently, neither God nor Satan espouses republican liberty. The poem suggests that the freedom created by God, although it relies on an arbitrary deity, is the only possible path to freedom.
An orphan gene is essential for efficient sperm entry into eggs in Drosophila melanogaster
Authors: Guay Sara Y , Patel Prajal H , Thomalla Jonathon M , McDermott Kerry L , O’Toole Jillian M , Arnold Sarah E , Obrycki Sarah J , Wolfner Mariana F , Findlay Geoffrey D 
Published: February 2025

Abstract
While spermatogenesis has been extensively characterized in the Drosophila melanogaster model system, very little is known about the genes required for fly sperm entry into eggs. We identified a lineage-specific gene, which we named katherine johnson (kj), that is required for efficient fertilization. Males that do not express kj produce and transfer sperm that are stored normally in females, but sperm from these males enter eggs with severely reduced efficiency. Using a tagged transgenic rescue construct, we observed that the KJ protein localizes around the edge of the nucleus at various stages of spermatogenesis but is undetectable in mature sperm. These data suggest that kj exerts an effect on sperm development, the loss of which results in reduced fertilization ability. Interestingly, KJ protein lacks detectable sequence similarity to any other known protein, suggesting that kj could be a lineage-specific orphan gene. While previous bioinformatic analyses indicated that kj was restricted to the melanogaster group of Drosophila, we identified putative orthologs with conserved synteny, male-biased expression, and predicted protein features across the genus, as well as likely instances of gene loss in some lineages. Thus, kj was likely present in the Drosophila common ancestor. It is unclear whether its role in fertility had already evolved at that time or developed later in the lineage leading to D. melanogaster. Our results demonstrate a new aspect of male reproduction that has been shaped by a lineage-specific gene and provide a molecular foothold for further investigating the mechanism of sperm entry into eggs in Drosophila.
Engagement of Older Adults in the Design, Implementation and Evaluation of Artificial Intelligence Systems for Aging: A Scoping Review
Authors: Cho Hannah , Oh Oonjee , Greene Nancy , Gordon Larissa , Morgan Sherry , Walke Lisa , Demiris George 
Published: February 2025

Abstract
Integration of artificial intelligence (AI) in health and healthcare, especially for older adults, has significantly advanced healthcare delivery. AI technologies, with capabilities such as self-learning and pattern recognition, are employed to address social isolation and monitor older adults' daily activities. However, rapid AI development often fails to consider the heterogeneous needs of older populations, which could exacerbate an existing digital divide and inequality. This scoping review examines older adults’ involvement in AI system design, implementation, and evaluation of AI systems in health and healthcare literature, emphasizing the necessity of their input for beneficial AI systems. We conducted a scoping review according to PRISMA-SCR. We reviewed 17 studies, finding that half of these studies (n = 8) engaged older adults during the design phase, a small number (n = 3) during the evaluation stage, and even fewer (n = 2) involved older adults in the implementation stage. Despite AI’s growing role, design processes often overlook older adults’ needs. Our findings emphasize the need for inclusive, participatory design approaches to address ethical and equity challenges, enhancing user engagement and relevance. We also highlight how these approaches address the needs of older adults and improve outcomes. Specifically, we integrated evidence showing the practical benefits of these approaches for better accessibility, usability, and engagement among older adults. While AI has potential to improve healthcare delivery, these approaches must be part of broader efforts to ensure ethical, inclusive, and equitable AI practices, especially in gerontology.
Promoting Housing Stability through Eviction Prevention for Older Adults in Social Housing: A Qualitative Study
Authors: Shepard Christine L , Um Seong-gee , Roche Brenda , Gould Sarah , Austen Andrea , Hitzig Sander L 
Published: February 2025

Abstract

Background and Objectives
Evictions are a major contributor to homelessness among low-income older renters, many of whom are living in social housing. Research indicates that social housing may protect individuals from eviction, but older adults may still be vulnerable, especially for non-payment of rent. This research used a qualitative approach to understand the factors that place older adults in social housing at risk of eviction and identify strategies to promote housing stability.

Research Design and Methods
Participants included older adult tenants in social housing in Toronto, Canada (n=58) as well as community-based health and social service providers (n=58) that operate in the buildings. Semi-structured interviews and focus groups explored (a) experiences with eviction; (b) challenges tenants face managing their tenancy; and (c) supports tenants need to maintain their housing.

Results
Most service providers had experiences supporting a tenant under threat of eviction. Tenants similarly had experiences with the eviction process, ranging from being threatened with a future eviction to receiving eviction notices and attending hearings with the landlord and tenant board. To understand experiences with evictions and opportunities to strengthen eviction prevention practices, we generated the following themes: (a) creating fear and mistrust through evictions; (b) identifying the underlying cause of an eviction; (c) ineffective tenancy management practices; and (d) proactive community supports.

Discussion and Implications
Current eviction prevention strategies were viewed as inadequate, and findings highlighted the need to transform supports to better meet the needs of low-income older tenants. This includes more proactive and "senior friendly” approaches and increased access to community support services to promote housing stability.
What Happens Behind Closed Doors? Investigating Care Practices in Nursing Home and Assisted Living Memory Care Units
Authors: Elliot Amy E , Bowblis John R , Nelson Ian , Menne Heather 
Published: February 2025

Abstract
In the United States, long-term care providers such as nursing homes and assisted living communities are meeting consumer demand through housing and care options designed to support the growing population of people living with dementia. One approach to providing dementia care is the development of “memory care units” within existing nursing homes and assisted livings. Memory care units provide a setting more tailored to the cognitive and functional abilities of these individuals. There is emerging evidence about the optimum strategies for memory care environments; however, little is known about the implementation, prevalence, and quality of practice- and environment-based strategies in memory care units. This article provides insight into the prevalence of memory care unit practices using data from Ohio. The analysis points to policy and practice opportunities to address the quality of life and care for people living with dementia.
The China national GeneBank sequence archive (CNSA) 2024 update
Authors: Wang Weiwen , Tan Cong , Li Ling , Li Xia , Zhang Lei , Li Xiaoqiang , Wang Jieyu , He Ziyi , Yang Tao , Ma Kailong , Hu Qingjiang , Yang Wenzhen , Li Zhiyong , Zhang Mingwen , Du Wensi , Yang Fan , Xu Zhicheng , Ma Xizheng , Tong Jiawei , Cai Jia , Hua Cong , Chen Fengzhen , You Lijin , Li Liang , Zeng Wenjun , Wang Bo , Xu Xun , Wei Xiaofeng 
Published: February 2025

Abstract
The China National GeneBank Sequence Archive (CNSA) is an open and freely accessible curated data repository built for archiving, sharing, and re-utilizing of multi-omics data. The remarkable advancement in sequencing technologies has triggered a paradigm shift in life science research. However, it also poses tremendous challenges for the research community in data manage and reusability. With the dramatic advance of sequencing technologies like spatial transcriptome sequencing, it brings an unprecedented explosion in sequence data and new requirements for data archiving. CNSA was established in 2017 as one of the fundamental infrastructures to offer multi-omics data archiving for the worldwide research community. Here, we present the state-of-the-art enhancements of CNSA encompassing the dramatical increase of varied types of data, the latest features and services implemented in CNSA as well as consistent efforts supporting global cooperation in biodiversity preservation and utilization. CNSA provides public archiving and open-sharing services for sequencing data and relevant metadata including genome, transcriptome, metabolism, and proteome from single-cell (also spatial resolved) level to individual and population level, as well as further analyzed results. As of 2024, CNSA has archived over 16.3 petabytes of data and provided the data curation, preservation, and open-share service for over 1,581 publications from over 560 institutions. It plays a pivotal role in supporting global scientific projects such as the 10,000 Plant Genomes Project. So far, CNSA has been recommended by various academic publishers such as Cell, Elsevier, and Oxford University Press. CNSA is accessible at https://db.cngb.org/cnsa/.
How cardiac output is controlled in a Fontan circulation: an update
Authors: Gewillig Marc , Salaets Thomas , Van de Bruaene Alexander , Van den Eynde Jef , Brown Stephen C 
Published: February 2025

Summary
After creating a Fontan circuit, control of the circulation is shifted upstream from the ventricle to the newly created Fontan portal system. The goal of this review was to illustrate that the customary laws of biventricular cardiac output no longer apply and explain why standardized cardiac failure treatment regimens have little or no effect on a failing Fontan patient. A Fontan circulation is, in effect, a circulation in series regulated by the basic rules of any hydrodynamic circuit. We developed a formula that elucidates how flow through the critical bottleneck, and therefore through the whole circuit, is controlled. The critical bottleneck in a hydrodynamic model is the prime determinant of overall flow; other (less critical) bottlenecks may control local upstream congestion, but not overall flow. Once relieved, control of flow shifts to the next most significant bottleneck. The available options for improving flow in a hydrodynamic model are identical to those applicable to any dam: tackle the obstruction (the most impactful approach), push harder upstream (the easiest action) or pull/suck further downstream of the bottleneck (the least efficient strategy). In the early stages, the Fontan neo-portal circulation plays a pivotal role in the pathophysiology. The ventricle has little effect and has an impact only at a late stage. The Fontan formula in the present article stands as a valuable tool, aiding physicians in comprehending the pathophysiological and hydrodynamic intricacies of the Fontan circuit within the context of everyday clinical practice.
Survival outcomes after surgery for type-a aortic dissection: a contemporary Dutch nationwide registry study
Authors: Timmermans Patrick T , Velders Bart J J , Groenwold Rolf H H , Vos Roemer J , Roefs Maaike M , Braun Jerry , Klautz Robert J M , Mecozzi Gianclaudio , Hjortnaes Jesper 
Published: February 2025

Abstract

OBJECTIVES
To describe the nationwide survival outcomes after surgery for type-A dissection (TAAD).

METHODS
All patients who underwent surgery for TAAD in the Netherlands between 2018 and 2021 were identified in the Netherlands Heart Registration (NHR) database. The NHR data were linked to lifelong survival data from Statistics Netherlands (98% match). Time trends for in-hospital and mid-term mortality were described, and age- and sex-adjusted regression analysis was performed. The cause and location of death were reported and stratified according to survival time intervals.

RESULTS
The study population consisted of 1317 patients with a mean age of 63.1 years (11.8). The number of surgeries increased from 284 in 2018 to 375 in 2021. The surgery included the ascending aorta in 99%, aortic arch in 73%, aortic root in 32.5% and the descending aorta in 5% of cases. In-hospital mortality decreased from 20.4% in 2018 to 13.9% (95% CI) in 2021. A total of 318 deaths were recorded and the majority (70%) of patients died from the consequences of their dissection. However, among 365-day survivors, only 14% died related to their dissection, while 37% of deaths were related to cardiovascular disease and 17% to cancer. The majority (84%) of patients died in the hospital but deaths after 365 days occurred most frequently (37%) at home.

CONCLUSIONS
Over the recent years, the number of surgeries for type-A dissections in the Netherlands has increased and in-hospital mortality has decreased. For patients surviving one year after surgery, the main cause of death was not dissection, but other causes such as cardiovascular disease or cancer.
The (Many) Afterlives of Transitional Justice: Practice-based Insights on Continuity, Impact and Evolving Justice Struggles
Authors: Destrooper Tine , Evrard Elke 
Published: February 2025

ABSTRACT∞
In the past decade, the paradigm of transitional justice has come under increasing scrutiny. Transitional justice scholarship has been characterized by self-reflective critiques, while on the ground even emblematic transitional justice cases have seen the (re)emergence of authoritarian tendencies, raising further questions about the paradigm’s legitimacy. Yet, in practice, a wide range of (grassroots) justice actors continue to invoke transitional justice as a mobilizing framework and action repertoire in an increasingly wide range of long and encompassing struggles for justice. These seemingly contradictory dynamics of crisis and expansion raise the question of how transitional justice lives on, both in a temporal and conceptual sense, years to decades after its initial deployment, and in the face of new socio-political and legal dynamics. This article builds on eight focus groups with 69 local justice actors from seven countries to present practitioner-informed insights about the many afterlives of transitional justice. Based on qualitative thematic analysis, we argue that these afterlives are shaped by plural, co-existing dynamics of continuity and ongoingness, unfolding through novel initiatives and processes across varying timelines, driven by the protagonism of victim-survivors, civil society and youth. This temporal stretching led practitioners to highlight the significance of intergenerational initiatives and impacts, to resituate the perceived legacies of early and standardized transitional justice interventions within protracted and evolving justice trajectories and to underscore the importance of the communicative functions and potential of transitional justice language and tools to bolster new and intersecting justice struggles.
Ophthalmic Manifestations of the Mpox Virus: A Systematic Review and Meta-Analysis
Authors: Rohilla Shivam , Gaidhane Shilpa , Balaraman Ashok Kumar , Padmapriya G , Kaur Irwanjot , Lal Madan , Iqbal Suhaib , Siva Prasad G V , Pramanik Atreyi , Vishwakarma Teena , Malik Praveen , Sharma Promila , Singh Mahendra Pratap , Yappalparvi Ambanna , Punia Ankit , Jagga Megha , Shabil Muhammed , Mehta Rachana , Sah Sanjit , Zahiruddin Quazi Syed , Serhan Hashem Abu , Bushi Ganesh 
Published: February 2025

Abstract

Background
Recent outbreaks of monkeypox (Mpox) have raised concerns about its complications, including ophthalmic manifestations such as conjunctivitis, keratitis, and potential vision impairment. The lack of comprehensive data on these ocular complications hinders the development of effective clinical guidelines. This review aim to synthesize existing evidence on the prevalence and characteristics of Mpox-related ocular complications.

Methods
A systematic literature search was conducted across PubMed, Embase, Web of Science, and Scopus, covering studies up to September 8, 2024. Studies focusing on conjunctivitis, keratitis, eye lesions, visual impairment, and other ophthalmic outcomes in Mpox cases were included. Meta-analyses were performed using a random-effects model to estimate pooled prevalence rates, with heterogeneity assessed using the I² statistic. Sensitivity analyses and publication bias assessments were also conducted.

Results
A total of 25 studies were included, with 22 contributing to the meta-analysis. The pooled prevalence of conjunctivitis in Mpox cases was 8.9% (95% CI: 4.4%–17.1%), keratitis 3.4% (95% CI: 1.4%–7.7%), eye lesions 3.4% (95% CI: 1.4%–7.7%), and visual impairment 4.3% (95% CI: 0.8%–20.6%). Other ocular manifestations had a pooled prevalence of 12.4% (95% CI: 0.6%–76.9%). Significant heterogeneity was observed, particularly for conjunctivitis and other ocular manifestations, suggesting variability in presentation.

Conclusion
Conjunctivitis is the most common ophthalmic complication of Mpox, followed by notable rates for keratitis, eye lesions, and visual impairment. These findings emphasize the need for early recognition, routine ocular exams, and effective management of Mpox-related eye complications. Further high-quality research is necessary to better understand and address these ocular complications.
Metabolic labour division trade-offs in denitrifying microbiomes
Authors: Roothans Nina , van Loosdrecht Mark C M , Laureni Michele 
Published: February 2025

Abstract
Division of metabolic labour is a defining trait of natural and engineered microbiomes. Denitrification—the stepwise reduction of nitrate and nitrite to nitrogenous gases—is inherently modular, catalysed either by a single microorganism (termed complete denitrifier) or by consortia of partial denitrifiers. Despite the pivotal role of denitrification in biogeochemical cycles and environmental biotechnologies, the ecological factors selecting for complete versus partial denitrifiers remain poorly understood. In this perspective, we critically review over 1500 published metagenome-assembled genomes of denitrifiers from diverse and globally relevant ecosystems. Our findings highlight the widespread occurrence of labour division and the dominance of partial denitrifiers in complex ecosystems, contrasting with the prevalence of complete denitrifiers only in simple laboratory cultures. We challenge current labour division theories centred around catabolic pathways, and discuss their limits in explaining the observed niche partitioning. Instead, we propose that labour division benefits partial denitrifiers by minimising resource allocation to denitrification, enabling broader metabolic adaptability to oligotrophic and dynamic environments. Conversely, stable, nutrient-rich laboratory cultures seem to favour complete denitrifiers, which maximise energy generation through denitrification. To resolve the ecological significance of metabolic trade-offs in denitrifying microbiomes, we advocate for mechanistic studies that integrate mixed-culture enrichments mimicking natural environments, multi-meta-omics, and targeted physiological characterisations. These undertakings will greatly advance our understanding of global nitrogen turnover and nitrogenous greenhouse gases emissions.