bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidencia de la formación y actualización docente en la formación integral del estudiante desde la socioformación
Authors: Parra Acosta Haydee , López Loya José 
Published: January 2024

Antecedentes: La formación de los médicos acorde a los retos del contexto global, demanda docentes innovados. Las pedagogías tradicionales necesitan ser reemplazadas por modelos pedagógicos que contribuyan al desarrollo de competencias desde la socioformación. Objetivo: Valorar cómo incide la formación y actualización docente en la mediación del aprendizaje y en la formación integral del estudiante desde la socioformación. Método: Estudio transversal con enfoque cuantitativo que se realizó mediante la aplicación de dos cuestionarios a una muestra representativa de 106 profesores y 171 estudiantes. El análisis de los cuestionarios se realizó mediante la estadística descriptiva e inferencial con un nivel de significancia de p> 0.001. Resultados: Existe correlación significativa entre las variables: los docentes se actualizan en el modelo educativo por competencias, analizan su intervención pedagógica y reconocen la necesidad de desarrollar competencias docentes, con las variables: coordinan con eficiencia el proceso formativo de los estudiantes, contribuyen a su formación integral, propician que los estudiantes desarrollen valores, trabajen las emociones positivas y disfruten lo que hagan. También se correlaciona con la competencia de mediación del aprendizaje orientada a: propiciar que resuelvan problemas y sean creativos e innovadores. Ello muestra que cuando la formación y actualización del docente incorpora procesos de análisis y reflexión, incide en la formación integral del estudiante desde la socioformación.
Unleash the Potential of Mystery, Ghost & Paranormal Tourism through the lens of locals in Kuldhara, Rajasthan, India: A qualitative study
Authors: SINGH KULDEEP , Sharma Reena , Bagri S.C. 
Published: January 2024

Mystery tourism is a novel topic related to travelling to events or destinations based on some stories, myths, beliefs of the host community or tourists. This also includes the myths and curiosity of locals and travelers about some known and unknown incidents or places. This paper aims to find out the experiences and perceptions of locals regarding mystery tourism in Kuldhara, Rajasthan. Ghost tours, paranormal investigations, and haunted stories have attracted many tourists to Kuldhara. In-depth interviews with 25 residents of Kuldhara, Rajasthan, reveal that residents have positive beliefs and ideologies regarding ghosts and paranormal activities. The study took in year (March 2022 to Feb 2023). The researchers identified purposive sampling and snow balling which has been used in previous studies too. This study is qualitative, and researchers employed thematic analysis techniques manually. Five major themes emerged from mystery tourism in Kuldhara, i.e., Myth & Tales, Perception of locals, Role of Government and Private bodies, Impact of mystery tourism and economic benefits. The study reveals that most locals face mysterious incidents and believe in ghosts and paranormal activities. This study generates curiosity among the readers and researchers to understand and explore the concepts of mystery tourism in a better way. Implications for developing mystery tourism attractions and support for resident well-being are offered.
Extracellular vesicles as next-generation therapeutics and biomarkers in amyloidosis: a new frontier
Authors: Phan Thanh Huyen , Reed Joanne H. 
Published: January 2024

Nanoparticles hold a great potential for therapeutic targeting due to their ability to improve the stability of encapsulated cargo and promote the transport of cargo across membranes to reach to the target site. Most commercially available nanomedicines are simple synthetic liposomes, however, there are numerous side effects due to their off-target delivery and rapid clearance from the bloodstream. Recently, attention has moved toward extracellular vesicles (EVs)–lipid bilayer enclosed particles released by cells (size ranging from 30 to 10,000 nm in diameter). EVs carry and transport lipids, proteins, and nucleic acids from their parental cells to recipient cells, hence they play a key role in intercellular communication. The ability of EVs to cross biological barriers including the blood brain barrier has generated significant attention to explore them as potential biomarkers and natural drug delivery vehicles for various therapeutics and small molecules. EVs have also been implicated in disease pathogenesis by transmitting pathogenic proteins between cells, making them promising biomarkers for disease diagnosis and monitoring. In this review, we will focus on the potential and challenges of EVs as biomarkers, drug delivery vehicles and next-generation therapeutics. Finally, we will explore misfolded protein disorders, amyloidosis, as a case study for how EVs may contribute to disease pathology and how EVs could be applied in the clinic as diagnostic and prognostic biomarkers of amyloid diseases.
Component-based estimation of recovery time and time-related expenses after hurricane events
Authors: Wei Zhuoxuan , Pinelli Jean-Paul , Gurley Kurtis , Hamid Shahid , Flannery Gail 
Published: January 2024

Introduction: Due to hurricane damage, building residents or businesses must be relocated during the recovery time, which leads to time-related expenses (TRE), also known as additional living expenses (ALE) or extra expense coverage (EEC) or business interruption insurance (BIC). TRE are difficult to predict since they depend on the damage and time necessary to repair the building as well as on external factors such as damaged utilities and the availability of labor and materials, among other issues.Methods: In this study, we developed a new TRE hurricane vulnerability model for mid/high-rise buildings. The model combines estimates of repair time (Trepair), delay time (Tdelay), and utilities downtime (Tdown) to predict overall recovery time (Treco).Results: The outputs of the model include 1) TRE vulnerability matrices, which yield probabilities of Trepair, Tdelay, Tdown, Treco, and TRE conditional on either maximum 3-s wind speed or overall building damage ratio; 2) the corresponding vulnerability curves, which yield the mean values as a function wind speed or damage ratio.Discussion: Insurers can use these results to project TRE, and emergency managers and urban planners can use the recovery times to characterize the resilience of coastal communities. This paper summarizes the methodology and illustrates its implementation and results. The selected results of Treco are compared with the recovery times provided using the HAZUS-MH Hurricane Model.
Expanding the genetic spectrum of mitochondrial diseases in Tunisia: novel variants revealed by whole-exome sequencing
Authors: Gouiza Ismail , Hechmi Meriem , Zioudi Abir , Dallali Hamza , Kheriji Nadia , Charif Majida , Le Mao Morgane , Galai Said , Kraoua Lilia , Ben Youssef-Turki Ilhem , Kraoua Ichraf , Lenaers Guy , Kefi Rym 
Published: January 2024

Introduction: Inherited mitochondrial diseases are the most common group of metabolic disorders caused by a defect in oxidative phosphorylation. They are characterized by a wide clinical and genetic spectrum and can manifest at any age. In this study, we established novel phenotype–genotype correlations between the clinical and molecular features of a cohort of Tunisian patients with mitochondrial diseases.Materials and methods: Whole-exome sequencing was performed on five Tunisian patients with suspected mitochondrial diseases. Then, a combination of filtering and bioinformatics prediction tools was utilized to assess the pathogenicity of genetic variations. Sanger sequencing was subsequently performed to confirm the presence of potential deleterious variants in the patients and verify their segregation within families. Structural modeling was conducted to study the effect of novel variants on the protein structure.Results: We identified two novel homozygous variants in NDUFAF5 (c.827G&gt;C; p.Arg276Pro) and FASTKD2 (c.496_497del; p.Leu166GlufsTer2) associated with a severe clinical form of Leigh and Leigh-like syndromes, respectively. Our results further disclosed two variants unreported in North Africa, in GFM2 (c.569G&gt;A; p.Arg190Gln) and FOXRED1 (c.1261G&gt;A; p.Val421Met) genes, and we described the first case of fumaric aciduria in a Tunisian patient harboring the c.1358T&gt;C; p.Leu453Pro FH variant.Conclusion: Our study expands the mutational and phenotypic spectrum of mitochondrial diseases in Tunisia and highlights the importance of next-generation sequencing to decipher the pathomolecular mechanisms responsible for these disorders in an admixed population.
Loss-of-function variant in the LRR domain of SLITRK2 implicated in a neurodevelopmental disorder
Authors: Afsar Tayyaba , Fu Hongxia , Khan Hammal , Ali Zain , Zehri Zamrud , Zaman Gohar , Abbas Safdar , Mahmood Arif , Alam Qamre , Hu Junjian , Razak Suhail , Umair Muhammad 
Published: January 2024

Background: Neurodevelopmental disorders are characterized by different combinations of intellectual disability (ID), communication and social skills deficits, and delays in achieving motor or language milestones. SLITRK2 is a postsynaptic cell-adhesion molecule that promotes neurite outgrowth and excitatory synapse development.Methods and Results: In the present study, we investigated a single patient segregating Neurodevelopmental disorder. SLITRK2 associated significant neuropsychological issues inherited in a rare X-linked fashion have recently been reported. Whole-exome sequencing and data analysis revealed a novel nonsense variant [c.789T&gt;A; p.(Cys263*); NM_032539.5; NP_115928.1] in exon 5 of the SLITRK2 gene (MIM# 300561). Three-dimensional protein modeling revealed substantial changes in the mutated SLITRK2 protein, which might lead to nonsense-medicated decay.Conclusion: This study confirms the role of SLITRK2 in neuronal development and highlights the importance of including the SLITRK2 gene in the screening of individuals presenting neurodevelopmental disorders.
Complex patterns on HEp-2 indirect immunofluorescence assay in a large sample referred for anti-cell autoantibodies detection
Authors: Santos Wilton Ferreira S. , Cantuária Ana Paula de Castro , Félix Daniele de Castro , Guimarães Natália Carvalho , de Melo Igor Cabral Santos 
Published: January 2024

IntroductionThe combination of patterns is a frequent and challenging situation in the daily laboratory routine of autoantibodies testing using HEp-2 cells indirect immunofluorescence assay (HEp-2-IFA). Recently, the Brazilian Consensus on Autoantibodies (BCA) named these combinations as complex patterns (CPs) and organized them into 3 subtypes: multiple, mixed, and composite. This study aimed to describe the most frequent combinations of HEp-2-IIF patterns according to this new nomenclature.MethodsRoutine HEp-2-IFA results reported in January and June 2017 were reviewed using the new BCA classification. Visual pattern recognition was performed by experts on HEp-2-IFA readings, using the International Consensus on Antinuclear Antibodies (ANA) Patterns (ICAP) and BCA recommendations.Results54,990 serum samples from different patients were tested for ANA-HEp-2, and 11,478 (20.9%) were positive at a titer ≥ 1/80. Among these positive samples, 1,111 (9.7%) displayed CPs, divided into 95 different combinations. A higher proportion of CPs was observed in the pediatric age group. Multiple, mixed, and composite patterns were present in 85.3, 5.4, and 9.5% of the samples, respectively. In the multiple/mixed pattern group (n=1,005), double, triple, and quadruple combinations (ICAP/BCA codes) were observed in 97.7%, 2.2%, and 0.1%, respectively. The double nuclear pattern was the most prevalent combination observed (67.6%). The most common CPs registered were AC-4 (nuclear fine speckled) + AC-6,7 (nuclear discrete dots) (n=264); AC-2 (nuclear dense fine speckled) + AC-6,7 (n=201); AC-4+AC-8,9,10 (nucleolar) (n=129); and AC-3 (centromere)+AC-4 (n=124). All of these combinations were in the multiple subgroup.ConclusionAlmost 10% of positive results in the HEp-2 procedure displayed CPs. Among the 3 subtypes of CPs proposed, the multiple pattern was the most prevalent, especially in the pediatric population. The AC-4, AC-2, and AC-6,7 were the most prevalent single patterns observed in the combinations described in this study. There was a significant association between age and the prevalence of most combined patterns. The AC-4+AC-6,7 combination was the most prevalent complex pattern detected regardless of the age group. The AC-2+AC-6,7 was more prevalent in younger individuals. The concepts involved in the CPs definition should add value to the reading and interpretation of the HEp-2-IIF assay.
Identifying biomarkers deciphering sepsis from trauma-induced sterile inflammation and trauma-induced sepsis
Authors: Papareddy Praveen , Selle Michael , Partouche Nicolas , Legros Vincent , Rieu Benjamin , Olinder Jon , Ryden Cecilia , Bartakova Eva , Holub Michal , Jung Klaus , Pottecher Julien , Herwald Heiko 
Published: January 2024

ObjectiveThe purpose of this study was to identify a panel of biomarkers for distinguishing early stage sepsis patients from non-infected trauma patients.BackgroundAccurate differentiation between trauma-induced sterile inflammation and real infective sepsis poses a complex life-threatening medical challenge because of their common symptoms albeit diverging clinical implications, namely different therapies. The timely and accurate identification of sepsis in trauma patients is therefore vital to ensure prompt and tailored medical interventions (provision of adequate antimicrobial agents and if possible eradication of infective foci) that can ultimately lead to improved therapeutic management and patient outcome. The adequate withholding of antimicrobials in trauma patients without sepsis is also important in aspects of both patient and environmental perspective.MethodsIn this proof-of-concept study, we employed advanced technologies, including Matrix-Assisted Laser Desorption/Ionization (MALDI) and multiplex antibody arrays (MAA) to identify a panel of biomarkers distinguishing actual sepsis from trauma-induced sterile inflammation.ResultsBy comparing patient groups (controls, infected and non-infected trauma and septic shock patients under mechanical ventilation) at different time points, we uncovered distinct protein patterns associated with early trauma-induced sterile inflammation on the one hand and sepsis on the other hand. SYT13 and IL1F10 emerged as potential early sepsis biomarkers, while reduced levels of A2M were indicative of both trauma-induced inflammation and sepsis conditions. Additionally, higher levels of TREM1 were associated at a later stage in trauma patients. Furthermore, enrichment analyses revealed differences in the inflammatory response between trauma-induced inflammation and sepsis, with proteins related to complement and coagulation cascades being elevated whereas proteins relevant to focal adhesion were diminished in sepsis.ConclusionsOur findings, therefore, suggest that a combination of biomarkers is needed for the development of novel diagnostic approaches deciphering trauma-induced sterile inflammation from actual infective sepsis.
Ultrasonography-guided radiofrequency ablation of vascular malformations—The moving shot technique
Authors: Sporns Peter B. , Psychogios Marios , Blackham Kristine , Zech Christoph , Wildgruber Moritz , Takes Martin 
Published: January 2024

PurposeTo describe a novel ultrasound-guided technique for percutaneous radiofrequency ablation of vascular malformations—the “moving shot technique.”MethodsPreliminary observational cohort study, conducted from June 1, 2019, to January 31, 2021, including all consecutive patients diagnosed with vascular malformations who were treated with ultrasound-guided radiofrequency ablation using the moving shot technique. Only patients who had undergone at least one unsuccessful previous treatment were included (sclerotherapy with ethanol/aethoxysklerol or embolization/surgery).ResultsEight patients with a median age of 22 years (interquartile range, 13–31) were included. Patients had different vascular malformations consisting of 1 arteriovenous malformation, 4 venous malformations, and 1 each a mixed venous-lymphatic malformation, a glomuvenous malformation and a FAVA (fibroadipose vascular anomaly). Malformations were located at the limbs in 5 patients (62.5%), the subcutaneous/intramuscular tissue of the body in 2 patients (25%) and at the chin in 1 patient (12.5%). Clinical symptoms were pain in 8 patients (100%), swelling in 6 patients (75%), and partial immobility in 4 patients (50%). All patients showed an improvement of clinical symptoms after treatment with 7 (87.5%) being completely asymptomatic and 1 (12.5%) showing improvement of immobility and pain. No procedural complications, such as nerve damage or skin burns occurred.ConclusionThe moving shot technique using ultrasonography-guided radiofrequency ablation is a promising technique for the interventional treatment of vascular malformations and should be validated in multicenter-approaches.
Electroencephalographic power spectrum changes in cerebral small vessel disease combined with cognitive dysfunction and its relationship with neutrophil/lymphocyte ratio and its clinical value – a pilot study
Authors: Guo Xiaomin , Liu Zongwei , Yuan Weishuai , Wei Aiqin , Luo Guogang 
Published: January 2024

ObjectiveThe study aimed to explore the changes in the electrical power spectrum of the brain and its correlation with neutrophil/lymphocyte ratio (NLR) in patients with cognitively impaired cerebral small vessel disease (CSVD) and to explore its clinical application.MethodsA total of 61 patients with CSVD who attended the People’s Hospital of Shaanxi Province from September 2021 to September 2022 were divided into the group with cognitive impairment (cerebral small vascular with cognitive impairment, CSVCI group, n = 29) and the group without cognitive impairment (CSVD group, n = 32) based on the Montreal Cognitive Assessment Scale (MoCA) score, while 20 healthy subjects were recruited as the control group (healthy control, HC group). EEG was performed in the three groups, and the difference in whole brain quantitative EEG power spectral density (PSD) was calculated and compared between the three groups.ResultsThe PSD values in the δ and θ bands of the CSVCI group were higher than those of the CSVD group, while the PSD values in the α band were lower than those of the CSVD and HC groups. In addition, PSD values in the δ-band in the CSVD group were lower than those in the HC group (all p &lt; 0.05). Multifactorial logistic regression showed that reduced α-band global average PSD and low years of education were independent risk factors for cognitive impairment in patients with CSVD (p &lt; 0.05). In patients with cerebral small-vessel disease, α-band PSD was positively and δ-band PSD negatively correlated with MoCA score, and paraventricular, deep white matter, and total Fazekas scores were negatively correlated with MoCA score. Furthermore, θ-band PSD is positively correlated with NLR (all p &lt; 0.05).ConclusionEEG activity was slowed down in patients with CSVD with cognitive impairment. The α-band global mean PSD values independently affected the occurrence of cognitive impairment in CSVD patients beyond the Fazekas score. NLR may be one of the mechanisms leading to the slowing down of the EEG, which can be used as an objective indicator for the early prediction of cognitive impairment but still needs to be clarified by further studies.
Ateroprotective effects of Plinia cauliflora in. New Zealand rabbits: beyond the lipid-lowering effect
Authors: Cestari Ana Paula , Gasparotto Francielly Mourão , Kassuya Cândida Aparecida Leite , Lacerda Tauany Milan Ribeiro , Donadel Guilherme , Moura Catia Sari , Ceranto Daniela Boleta , Jacomassi Ezilda , Alberton Odair , Tramontini Salviano Belletini , Bertoncello Luana Ale , Gasparotto Junior Arquimedes , Lourenço Emerson Luiz Botelho 
Published: January 2024

Introduction:Plinia cauliflora [Mart.] Kausel (Myrtaceae), popularly known as “jabuticaba,” is a fruit species native to Brazil. Despite extensive widespread usage, its antiatherosclerotic properties' impact remains unknown. Thus, the present study aimed to investigate the cardioprotective effects of a preparation obtained from the fruit peels of P. cauliflora (EEPC).Methods: Male New Zealand rabbits received a 1% cholesterol-supplemented diet for 60 days. On the thirtieth day, the animals were divided into five experimental groups and received, once a day, by the oral route, the EEPC (10, 30, and 100 mg/kg), simvastatin (2.5 mg/kg), or vehicle for 30 days. At the end of the experimental period, peripheral blood and arterial branch samples were collected. The levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), malondialdehyde (MDA), nitrotyrosine (NT), nitrite, interleukin 1 beta (IL-1b), interleukin 6 (IL-6), soluble inter-cellular adhesion molecule-1 (sICAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1) levels were measured. Moreover, the catalase and superoxide dismutase levels were measured on the arterial samples. Histopathological analysis and arterial morphometry were also performed.Results and discussion: The oral administration of ESEG significantly lowered the levels of lipids in rabbits that were fed a CRD diet. This treatment also adjusted the protective system against oxidation in the arteries by decreasing the oxidation of lipids and proteins. Additionally, the levels of IL-1b, IL-6, sICAM-1, and sVCAM-1 in the bloodstream decreased significantly, and this was accompanied by a reduction of atherosclerotic lesions in all branches of the arteries. The findings suggest that EEPC may be a possible option for additional management of atherosclerosis.
Salicylic acid alleviates the effects of cadmium and drought stress by regulating water status, ions, and antioxidant defense in Pterocarya fraxinifolia
Authors: Torun Hülya , Cetin Bilal , Stojnic Srdjan , Petrík Peter 
Published: January 2024

IntroductionPterocarya fraxinifolia (Poiret) Spach (Caucasian wingnut, Juglandaceae) is a relict tree species, and little is known about its tolerance to abiotic stress factors, including drought stress and heavy metal toxicity. In addition, salicylic acid (SA) has been shown to have a pivotal role in plant responses to biotic and abiotic stresses.MethodsThe current study is focused on evaluating the impact of foliar application of SA in mediating Caucasian wingnut physiological and biochemical responses, including growth, relative water content (RWC), osmotic potential (Ψs), quantum yield (Fv/Fm), electrolyte leakage, lipid peroxidation, hydrogen peroxide, and antioxidant enzymes, to cadmium (Cd; 100 µM) and drought stress, as well as their interaction. Moreover, the antioxidant activity (e.g., ascorbate peroxidase, catalase, glutathione reductase, peroxidase, and superoxide dismutase activities) of the stressed trees was investigated. The study was conducted on 6-month-old seedlings under controlled environmental conditions in a greenhouse for 3 weeks.Results and discussionLeaf length, RWC, Ψs, and Fv/Fm were decreased under all treatments, although the effect of drought stress was the most pronounced. An efficient antioxidant defense mechanism was detected in Caucasian wingnut. Moreover, SA-treated Caucasian wingnut plants had lower lipid peroxidation, as one of the indicators of oxidative stress, when compared to non-SA-treated groups, suggesting the tolerance of this plant to Cd stress, drought stress, and their combination. Cadmium and drought stress also changed the ion concentrations in Caucasian wingnut, causing excessive accumulation of Cd in leaves. These results highlight the beneficial function of SA in reducing the negative effects of Cd and drought stress on Caucasian wingnut plants.