bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Endoscopic Aspirator on Gasless Transoral Endoscopic Thyroidectomy Vestibular Approach
Authors: Shao Shuai , Zeng Qinxin , Yu Huimin , Wang Haobin , Wu Jian , Wu Guoyang 
Published: November 2023

Abstract

Background Transoral endoscopic thyroidectomy vestibular approach (TOETVA) is gradually becoming mature.At the same time, the disadvantages of CO2 inflatable TOETVA are also widely recognized, and there is no uniform standard alternative.Here, we report our initial experience and discuss the safety and effectiveness of maintaining the operative space with endoscopic suction support in TOETVA. Methods We retrospectively analyzed 60 patients treated with our laparoscopic aspirator-supported TOETVA from March 2022 to June 2023.We extended the three-hole endoscopic suction device from the median incision of the oral vestibule into the operation space, pressed it down, and fixed it to support and maintain the stability of the space.Evaluate its surgical safety and prognosis. Results The study included 60 patients who were treated with endoscopic aspirator-supported TOETVA.The average operation time was 141. 14 ± 36. 15min.The average number of dissected lymph nodes was 4. 67 ± 3. 75.Two of them were converted to laparotomy, and no serious postoperative complications occurred in any of the patients.Postoperative complications included 1 case of transient recurrent laryngeal nerve paralysis, 1 case of transient hypoparathyroidism, and 8 cases of transient lower lip numbness.There was 1 case of postoperative skin injury and 4 cases of postoperative skin ecchymosis.Two cases of temporary loss of smell or taste recovered within 1–3 months.There is no evidence of specific complications associated with the use of suction to support the surgical space. Conclusion The operation space of TOETVA is supported by a laparoscopic suction device, which is easy to operate, convenient to obtain materials, and optimizes the clarity of the surgical field, which has the potential for wide application.
Characterization of a suspension Vero cell line for viral vaccine production
Authors: Pain Bertrand , Bourigault Lea , Bresson Corinne , Jean Christian , Chevalard Christophe , Kloutz Mathilde , Soulet Damien , Pelissier Fleurine , Richard Stéphanie , Bassard Isabelle , Sève Nicolas , Charretier Cédric 
Published: November 2023

Abstract
Vero cells, as approved by the World Health Organization, have been the most commonly used continuous cell line for viral vaccine production over the last 25 years, but their adherent phenotype continues to limit productivity. Adapting to a suspension culture would overcome this restriction and reduce production costs. First, a Vero suspension isolate was obtained and metabolically characterized. Second, RNA sequencing analysis was used to identify differentially expressed genes between adherent and suspension cells, which revealed complete downregulation of adhesion and matrix-associated genes. Additionally, signaling pathways involving Wnt and other tyrosine kinase receptors were identified as potential leads for growth optimization. In particular, supplementation with fibroblast growth factor 2 allowed for a 20% increase in cell density. Finally, a comparative viral productivity assay revealed a 30% increase in poliovirus production in suspension Vero cells compared to adherent cells depending on the serotype, as well as a 140% increase in respiratory syncytial virus production and a 150% increase in yellow fever virus production. This work establishes the potential of the suspension Vero cell line as a new cell platform for viral vaccine production.
Risk Assessment and Predicting Homelessness and Police Interaction in Calgary Through Administrative Health Care Data
Authors: Shahidi Faezehsadat , MacDonald M. Ethan , Seitz Dallas , Messier Geoffrey 
Published: November 2023

Abstract

Background: Mental illness can lead to adverse outcomes such as homelessness and police interaction and understanding of the events leading up to these adverse outcomes is important.. Predictive machine learning (ML) models may not outperform logistic regression (LR).
Method: An administrative healthcare dataset was used, comprising of 240,219 individuals in Calgary, Alberta, Canada who were diagnosed with addiction or mental health (AMH) between April 1, 2013, and March 31, 2018. The cohort was followed for 2 years to identify factors associated with homelessness and police interactions. We used a univariable and a multivariable LR model to identify predictive factors of homelessness and police integration by estimating odds ratios (ORs) with a 95% confidence interval. Then LR and ML models, including random forests (RF), and extreme gradient boosting (XGBoost) were compared.
Results: After excluding prior outcomes before March 31, 2018, the cohort size decreased. Among 237,602 individuals, 0.8% (1,800) experienced first homelessness, while 0.32% (759) reported initial police interaction among 237,141 individuals. Male sex (AORs: H=1.51, P=2.52), substance disorder (AORs: H=3.70, P=2.83), psychiatrist visits (AORs: H=1.44, P=1.49), and drug abuse (AORs: H=2.67, P=1.83) were associated with initial homelessness (H) and police interaction (P). LR model with multinomial features, normalized data, and balanced classes showed superior performance (sensitivity =85%, AUC =84% for initial homelessness, and sensitivity =88%, AUC=81% for initial police interaction).
Conclusion: This study identified key features associated with initial homelessness and police interaction and demonstrated the superior performance of the LR models using multinomial features, normalized data, and balanced classes.
Top Health Service Concerns: A Data Mining Study of the Shanghai Health Hotline
Authors: Shi Lili , Zhao Tong , Shi Shimiao , Tan Tianyu , Regmi Aksara , Cai Yuyang 
Published: November 2023

Abstract

Objective
Our study aims to explore the health service issues of public concern through analyzing the basic characteristics of callers and information from health service work orders. The findings of this study will provide a reference to relevant government departments and assist the government in optimizing the allocation of health resources.
Methods
We used 16,962 original work orders from the Health Hotline in Shanghai since 2015 as our research data. We applied natural language processing to analyze the work orders. We conducted data cleaning on the work orders' textual content, and established a work order database. We performed analysis on call duration, content, purpose, topic, and other dimensions, using the 12320 Health Hotline call records from Shanghai in 2015.
Results
Out of the calls, they were categorized into four main groups: complaints, suggestions, inquiries and requests for assistance. Among these calls, the most common category was complaints with a total of 8,669 (51.11%), followed by help-seeking with 3335 (19.66%), consulting with 2727 (16.08%) and comments and opinion suggestions with 1484 (8.75%). The analysis of the data also showed that 6,689 calls (56.88%) from men outnumbered 5,071 calls (43.12%) from women, with 2,126 calls from parents (56.84%) slightly outnumbered 1,614 calls from children (43.16%). Furthermore, the top 10 health service concerns in Shanghai included attitudes towards services, medications, fees, registration, family planning, medical disputes, ambulances, environmental health, illegal medical practices, and immunization.
Conclusions
The concerns reflected by the Shanghai Health Hotline mirror the genuine health service needs of residents, carrying significant implications for urban health governance. These top 10 health service concerns in Shanghai hold paramount importance. They provide valuable insights for relevant government departments to implement targeted measures aimed at enhancing the quality of health services.
Isolated Microspore Culture for Embryoid Production in Artemisia annua (L)
Authors: Purnamaningsih Ragapadmi , Dewi Iswari Saraswati , Sukmadjaya Deden , Apriana Aniversari , Purwoko Bambang Sapta 
Published: November 2023

Abstract
The haploidy technique is a useful tool to produce pure, fully homozygous lines in a short time. Artemisia annua (L) is a medicinal plant that produces artemisinin widely known as an anti-malarial drug. Due to its very small flower (< 3.0 mm), this study aimed to conduct microscopic observations to determine the type of flowers and their corresponding microspore development stages suitable for microspore embryogenesis and to obtain embryoids from isolated microspore cultures. Media to induce embryoid production were based on NLN medium with 13% or 17% sucrose and the addition of plant growth regulators as follows: (1) a combination of NAA and BA, designated as MCA media; and (2) a combination of 2,4-D and Kinetin, designated as MCAD media. The result indicated that due to their proportion of uninucleate and binucleate microspore stages, flowers at about to bloom (AB) and early bloom (EB) stages contained sufficient microspore stages of late uninucleate to early binucleate microspores suitable to induce embryogenesis. The production of embryoid is faster in MCA13 and MCA17 than those of MCAD13 and MCAD17. MCA13 and MCAD13 gave rise to abundant callus-like structures than those of MCA17 and MCAD17. It is indicated that the addition of 2.4-D in MCAD media inhibited the growth of the embryoid, due to the formation of a white-gluey structure covered and mixed with the embryoid.
Macrophages modulate fibrosis during newt lens regeneration
Authors: Tsissios Georgios , Sallese Anthony , Perez-Estrada J. Raul , Tangeman Jared A. , Chen Weihao , Smucker Byran , Ratvasky Sophia C. , Grajales-Esq Erika l , Martinez Arielle , Visser Kimberly J. , Araus Alberto Joven , Wang Hui , Simon Andras , Yun Maximina H. , Rio-Tsonis Katia Del 
Published: November 2023

Abstract

Background Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Methods Here we generated a transgenic newt reporter line in which macrophages can be visualizedin vivo. Using this new tool, we analyzed the location of macrophages during lens regeneration. We uncovered early gene expression changes using bulk RNAseq in two newt species,Notophthalmus viridescensandPleurodeles waltl. Next, we used clodronate liposomes to deplete macrophages, which inhibited lens regeneration in both newt species. Results Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increase in apoptosis. Some of these phenotypes persisted for at least 100 days and could be rescued by exogenous FGF2. Re-injury alleviated the effects of macrophage depletion and re-started the regeneration process. Conclusions Together, our findings highlight the importance of macrophages in facilitating a pro-regenerative environment in the newt eye, helping to resolve fibrosis, modulating the overall inflammatory landscape and maintaining the proper balance of early proliferation and late apoptosis.
Biomarkers of oxidative stress and bioaccumulation of trace metals in waterbirds from different rice fields systems
Authors: Bergmann Fabiane Borba , Leitemperger Jossiele Wesz , Jorge Marianna Basso , Amaral Hugo Leonardo Cunha , Bianchini Adalto , Loro Vania Lucio , Guadagnin Demetrio Luis 
Published: November 2023

Abstract

Trace metals like copper (Cu) and zinc (Zn), essential at low concentrations but toxic in excess, contrast with non-essential metals like cadmium (Cd) and lead (Pb), significant environmental contaminants. Conventional and organic agriculture employ varying agrichemical inputs, including trace metals. Elevated trace metals in aquatic environments can harm waterbirds, with oxidative metabolism changes indicating environmental stress. We compared trace metal concentrations (Pb, Zn, Cd, Cu) in feathers and biomarkers in muscle and liver tissue of waterbirds from organic and conventional irrigated rice farms in South Brazil. Biomarkers included catalase (CAT) and superoxide dismutase (SOD) activity, protein carbonylation (PC), lipid peroxidation (LPO), and non-protein thiols (NPSH) concentration. Cu and Pb concentrations were higher in waterbirds from conventional farms, whereas Cd and Zn levels were elevated in organic farms. Birds foraging in organic farms exhibited lower CAT and SOD activity and higher NPSH, LPO, and PC concentrations compared to those in conventional farms. Higher Cu and Pb concentrations were associated with elevated SOD and CAT activity, more prevalent in birds frequenting conventional farms. Conversely, birds with higher Cd and Zn concentrations showed increased NPSH, LPO, and PC levels and were more common in organic farms. Our study underscores complex differences in trace metal accumulation and oxidative stress biomarkers among waterbirds in organic and conventional rice crops.
Enhancement of in vitro production of tropane alkaloids and phenolic compounds in Hyoscyamus niger by culture types and elicitor treatments
Authors: ALBAYRAK ilknur , DEMIRCI Tunahan , BAYDAR Nilgün GÖKTÜRK 
Published: November 2023

Abstract
This research aimed to identify the influences of 24-epibrasinolide (EBL) and methyl jasmonate (MJ) applications on growth parameters and secondary metabolite syntheses in adventitious root and cell suspension cultures of Hyoscyamine niger. Therefore, EBL at different concentrations (0.5, 1, and 2 mg L− 1), individually and together with 1 mM MJ were implemented in root and cell suspension cultures. In root cultures, the optimum concentration for achieving the highest root growth parameters was found to be 2 mg L− 1 of EBL. On the other hand, in cell suspension cultures, the best cell growth was observed when 1 mg L− 1 of EBL was applied. In root cultures, the highest scopolamine and hyoscyamine amounts were obtained when 1 mM MJ was applied in combination with EBL at concentrations of 1 and 2 mg L− 1, respectively. In cell cultures, 2 mg L− 1 EBL for scopolamine and 0.5 mg L− 1 EBL for hyoscyamine were found to be the best among all applications. Compared to the control, elicitor applications also stimulated phenolic production in both roots and cells, depending on their combinations and concentrations. Additionally, it was also found that the metabolite production performance of adventitious roots was higher than that of cells. In conclusion, it was suggested that the use of MJ and EBL could be a promising strategy for enhancing the accumulation of scopolamine, hyoscyamine, and phenolic substances in root and cell cultures of H. niger.
Study protocol for the Heads-Up trial: A phase II randomized controlled trial investigating head-up tilt sleeping to alleviate orthostatic intolerance in Parkinson’s Disease and parkinsonism
Authors: Stam Amber H. van der , de Vries Nienke M. , Shmuely Sharon , Smeenk Daan , Rutten Joost H. , Rossum Ineke A. van , de Bot Susanne T. , Claassen Jurgen A. , Bloem Bastiaan R. , Thijs Roland D. 
Published: November 2023

Abstract
Background
In persons with Parkinson’s disease (PD) or certain forms of atypical parkinsonism, orthostatic hypotension is common and disabling, yet often underrecognized and undertreated. About half of affected individuals also exhibit supine hypertension. This common co-occurrence of both orthostatic hypotension and supine hypertension complicates pharmacological treatments as the treatment of the one can aggravate the other. Whole-body head-up tilt sleeping (HUTS) is the only known intervention that may improve both. Evidence on its effectiveness and tolerability is, however, lacking, and little is known about the implementability.
Methods
In this double-blind multicenter randomized controlled trial (phase II) we will test the efficacy and tolerability of HUTS at different angles in 50 people with PD or parkinsonism who have both symptomatic orthostatic hypotension and supine hypertension. All participants start with one week of horizontal sleeping and subsequently sleep at three different angles, each maintained for two weeks. The exact intervention will vary between the randomly allocated groups. Specifically, the intervention group will consecutively sleep at 6°, 12° and 18°, while the delayed treatment group starts with a placebo angle (1°), followed by 6° and 12°. We will evaluate tolerability using questionnaires and compliance to the study protocol. The primary endpoint is the change in average overnight blood pressure measured by a 24-hour ambulatory blood pressure recording. Secondary outcomes include orthostatic blood pressure, orthostatic tolerance, supine blood pressure, nocturia and various other motor and non-motor tests and questionnaires.
Discussion
We hypothesize that HUTS can simultaneously alleviate orthostatic hypotension and supine hypertension, and that higher angles of HUTS are more effective but less tolerable. The Heads-Up trial will help to clarify the effectiveness, tolerability and feasibility of this intervention at home and can guide at-home implementation.
Trial registration:
ClinicalTrials.gov NCT05551377; Date of registration: September 22, 2022.
Changing trends in human brucellosis in pastoral and agricultural China, 2004–2019: A Joinpoint regression analysis
Authors: Ma Xiaojuan Ma , Liu Zhuo , Zhao Yuan , Geng Yuhui , Meng Hua , Chen Mingbo , Pan Ting , Pan Dongfeng , liang Peifeng 
Published: November 2023

Abstract

Background
Brucellosis, a zoonotic disease caused by Brucella species, poses a significant public health concern in China, particularly in pastoral and areas. The incidence of human brucellosis has risen sharply in China but little is known about trends in pastoral and agricultural areas respectively.
Methods
Annual and monthly incidence and numbers of reported cases of human brucellosis in regional China between 2004 and 2019 were accessed from the National Public Health Science Data Sharing Center, and the annual percentage change (APC) in pastoral and agricultural areas was calculated. Annual and monthly trends were analyzed by the Joinpoint regression model.
Results
569016 cases of human brucellosis were reported between 2004 and 2019 with a peak in 2014. A trend of increasing incidence was found (AAPC = 6.3%, 95% CI: 3.2–9.4%) with higher rates in pastoral areas but greater mean increases in agricultural (AAPC = 9.4) and agricultural/pastoral areas (AAPC = 7.7) than in pastoral areas (AAPC = 5.9). Infections occurred throughout the year with a concentration of cases between March and July. Increasing monthly incidence was found in all types of areas from January to May (APC > 0) and a decreasing monthly trend was seen from May to December (APC < 0, p < 0.05).
Conclusions
The incidence of human brucellosis was higher in pastoral areas of China than in agricultural/pastoral or agricultural areas, however, the rate of increase was higher in agricultural/pastoral and agricultural areas than in pastoral areas. Epidemic surveillance should be strengthened in key counties (cities, districts) and prevention and control measures increased nationwide.
PAFAH1B3 is a KLF9 target gene and promotes proliferation and metastasis in pancreatic cancer
Authors: Dong Cairong , Yao Jinping , Wu Zhipeng , Hu Junwen , Sun Liang , Wu Zhengyi , Yan Jinlong , Yin Xiangbao 
Published: November 2023

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human malignancies. Unlimited proliferation, invasion and migration of pancreatic cancer cells are the fundamental causes of death in PDAC. Previous studies by our group have shown that KLF9 inhibits the proliferation, invasion and migration of pancreatic cancer cells. However, the mechanisms are not fully understood. In this study, we found that platelet-activating factor acetylhydrolase IB3 (PAFAH1B3) was highly expressed in pancreatic cancer tissues and cells. In vitro and in vivo studies showed that overexpression of PAFAH1B3 promoted the proliferation and invasion of pancreatic cancer cells, while downregulation of PAFAH1B3 inhibited the proliferation and invasion of pancreatic cancer cells. Mechanistically, KLF9 expression was negatively correlated with PAFAH1B3 expression in pancreatic cancer tissues and cells. Western blotting showed that KLF9 negatively regulated the expression of PAFAH1B3 in pancreatic cancer tissues and cells. Rescue experiments showed that overexpression of PAFAH1B3 could partially rescue the reduction in pancreatic cancer cell proliferation, invasion and migration induced by KLF9 overexpression. Finally, chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays were carried out, and the results showed that KLF9 directly bound to the promoter of PAFAH1B3 and inhibited its transcriptional activity. In conclusion, our study indicated that KLF9 can inhibit the proliferation, invasion, migration and metastasis of pancreatic cancer cells by inhibiting PAFAH1B3.
Are rot-causing Botryosphaeriaceae species surviving in healthy Manihot esculenta propagative material in Brazil?
Authors: Brito Amanda Cupertino de Queiroz , de Mello Juliana Ferreira , Neto José Vitorino da Silva , de Assis Daniele Magna Azevedo , Souza Ana Elisa de Almeida , Costa Antonio Félix da , Lopes Ueder Pedro , de Souza-Motta Cristina Maria , Machado Alexandre Reis 
Published: November 2023

Abstract
The study aimed to verify the presence of latent pathogens from the Botryosphaeriaceae family, associated with black root rot and stem dry rot, in healthy propagative material of Manihot esculenta in Brazil. To this end, we investigated the composition of endophytic fungal communities present in fresh healthy stems collected from M. esculenta plantations. Forty-one genera, two families and one subclass of fungi were identified in the collected samples. We detected the species of Botryosphaeriaceae - Lasiodiplodia euphorbiaceicola, L. theobromae and Neoscytalidium dimidiatum - composing fungal endophytic communities in M. esculenta plantations close to the coast of the state of Pernambuco (Brazil). Pathogenicity tests carried out on seedlings and roots of M. esculenta confirmed the capacity of the Botryosphaeriaceae specimens obtained to cause lesions in this host. L. euphorbiaceicola, L. theobromae and N. dimidiatum are recognized as pathogenic for M. esculenta, responsible for causing black root rot and stem dry rot in this crop in Brazil and other countries. However, this is the first study to isolate them as endophytes in healthy propagative material, showing evidence that these Botryosphaeriaceae can survive as pathogens or endophytes in this crop in Brazil.