bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and identification of type 1d CP bovine viral diarrhea virus from Inner Mongolia, China
Authors: Zhao Fengmiao , Hao Luyao , Guo Ting , Zhou Yaping , Zhao Hongmei , Gao Yuan , Hao Yongqing 
Published: December 2023

Abstract
Background
In recent years, outbreaks of respiratory and gastrointestinal diseases in cattle is associated with bovine viral diarrhea virus (BVDV). BVDV infection is a persistent global issue, particularly concerning for calves health. Comprehensive epidemiological surveillance and research into its pathogenic characteristics are essential for effective control and prevention of this virus. Despite limited investigation into the epidemiology of BVDV in Inner Mongolia, there is a lack of comprehensive information in this area.
Results
From 2021 to 2022, within cattle farms implementing eradication plans for bovine viral diarrhea virus (BVDV), we detected 33 cases of BVDV nucleic acid positivity out of 103 samples (positive rate 32.04%). We isolated five strains of NCP-type BVDV (separation rate 15.15%) and one strain of CP-type BVDV (separation rate 3.03%). This CP BVDV strain was successfully isolated from the nasal swab of a calf with severe clinical symptoms in Hohhot, Inner Mongolia. Subsequently, we designated this strain as HH839 in this study. Non-cytopathic BVDV strains are common and participate in severe clinical acute infections, whereas cytopathic BVDV strains are rare and usually associated with mucosal disease outbreaks. Therefore, this study determined the biological characteristics of CP BVDV 1d strain HH839 using various assays, including cytopathic conditions, electron microscopy, serum neutralization test, whole-genome sequencing, sequence alignment, and replication kinetics. A cytopathic virus strain was isolated, and viral particles were observed within 40–60 nm using transmission electron microscopy. The growth curve showed that the virus propagated effectively in MDBK cells. Phylogenetic analyses based on the 5’untranslated region (5’UTR) and full-length genome sequences indicated that HH839 belonged to the BVDV-1d group and that the strain was in the same branch and had the closest genetic relationship with BJ-1308 (KT951841.1). New Zealand white rabbits were used to study the pathogenicity of different biotypes of BVDV, including the CP-type BVDV-infected group, NCP-type BVDV-infected group, and CP-type NCP-type BVDV mixed-infected group. The animal infection experiments showed that hematology and histopathology showed different degrees of changes, although the infected rabbits did not show typical disease symptoms.
Conclusion
We isolated a BVDV HH839 strain that can cause severe clinical symptoms in cattle. Genome-wide determination and infection experiments with HH839 provide material to further explore the regional prevalence of BVDV. We propose to reinforce the epidemiological surveillance of this virus.
Comprehensive pan-cancer analysis reveals SIRT5 is a predictive biomarker for prognosis and immunotherapy response
Authors: Ji Yacong , Li Chongyang , Wan Sicheng , Zhang Kui , Liu Yaling , Shi Shaomin 
Published: December 2023

Abstract
Background: SIRT5, a promising therapeutic target, modulates diverse cellular metabolic pathways. Its role in cancer remains elusive, necessitating a comprehensive pan-cancer analysis to elucidate its diagnostic, prognostic, and immunological significance.
Methods: We conducted an extensive investigation into SIRT5's involvement in tumorigenesis, diagnosis, prognosis, metabolic pathways, the immune microenvironment, and therapeutic responses across multiple cancers. Chemical modifiers of SIRT5 expression were explored, along with the relationship between SIRT5 and drug sensitivity. Furthermore, in vitro and in vivo experiments assessed SIRT5's role in melanoma.
Results: Differential expression of SIRT5 exhibited early diagnostic potential across various tumors, influenced by somatic cell copy number alterations and DNA methylation. SIRT5 expression correlated with clinical features and distinct metabolic, metastasis-related, and immune pathways. High SIRT5 levels predicted poor prognosis and impacted drug sensitivity. Additionally, SIRT5 expression correlated significantly with immune modulation, lymphocyte subpopulation infiltration, and immunotherapeutic response biomarkers. Varied SIRT5 expression was observed in immunotherapy cohorts. A range of chemicals affecting SIRT5 expression was identified. Notably, our findings underscore SIRT5 as a pivotal pathogenic factor promoting melanoma progression.
Conclusion: Our study presents a comprehensive analysis of SIRT5 and its regulatory genes, highlighting its robust diagnostic and prognostic potential across diverse cancers. SIRT5 may remodel the tumor microenvironment and holds promise for guiding immunotherapy responses. This underscores the potential of SIRT5-based cancer therapies.
Innovative dry electrode-based Health Patch: validating heart rate, respiration rate and user experience for vital signs monitoring
Authors: Wei Jonathan CJ , Broek Tim van den , Baardewijk Jan Ubbo van , Stokkum Robin , Kamstra Regina JM , Rikken Lars , Gijsbertse Kaj , Uzunbajakava Natallia Eduarda , Brink Willem van den 
Published: December 2023

Abstract

Successful implementation of remote monitoring of vital signs outside of the hospital setting hinges on addressing three crucial unmet needs: longer-term wear, skin comfort and signal quality. Earlier, we developed a Health Patch research platform that uses self-adhesive dry electrodes to measure vital digital biomarkers. Here, we report on the analytical validation for heart rate, heart rate variability and respiration rate. Study design included n = 25 adult participants with data acquisition during a 30-minute exercise protocol involving rest, squats, slow, and fast cycling. The Shimmer3 ECG Unit and Cosmed K5, were reference devices. Data analysis showed good agreement in heart rate and marginal agreement in respiratory rate, with lower agreement towards higher respiratory rates. The Lin's concordance coefficient was 0.98 for heart rate and 0.51 for respiratory rate. Heart rate variability (RMSSD) had a coefficient of 0.85. Participants generally expressed a positive experience with the technology, with some minor irritation from the medical adhesive. The results highlighted potential of this technology for short-to-medium term clinical use for cardiorespiratory health, due to its reliability, accuracy, and compact design. Such technology could become instrumental for remote monitoring providing healthcare professionals with continuous data, remote assessment and enhancing patient outcomes in cardiorespiratory health management.
Voluntary Unemployment Insurance
Authors: Larsen Birthe , Waisman Gisela 
Published: December 2023

Abstract
In countries with voluntary unemployment insurance, some workers are not insured against the risk of unemployment. This paper set-up a novel theoretical model in order to examine the impact on welfare and inequality when unemployment insurance is not obligatory but instead an individual decision. Workers are heterogeneous in terms of initial wealth, which affects the social assistance they can get. The higher the worker’s wealth level, the lower social assistance in case of unemployment, which therefore increases the incentive to obtain unemployment insurance. We consider an economy inhabited by risk averse workers and then expand the model to include educational choice. We analyse the mechanisms by which a higher social assistance and unemployment insurance for educated and uneducated workers impact unemployment, wages, education, inequality and welfare. We examine the choice of social security system considering different policy goals. An important one being to reduce inequality.
JEL codes: J6, I24
Primary data on symptom burden and quality of life among elderly patients at risk of dying during unplanned admissions to an NHS hospital: a cohort study using EuroQoL and the Integrated Palliative Care Outcome Scale
Authors: Johnston Bridget M. , Miller Mary , Normand Charles , Cardona Magnolia , May Peter , Lowney Aoife C 
Published: December 2023

Abstract
Background
Older people account heavily for palliative care needs at the population level and are growing in number as the population ages. There is relatively little high-quality data on symptom burden and quality of life, since these data are not routinely collected, and this group are under-recruited in primary research. It is unclear which measurement tools are best suited to capture burdens and experience.
Methods
We recruited a cohort of 221 patients aged 75 + years with poor prognosis who had an unplanned admission via the emergency department in a large urban hospital in England in 2019 and early 2020. Risk of dying was assessed using the CriSTAL tool. We collected primary data and combined these with routine health records. Baseline clinical data and patient reported quality of life outcomes were collected on admission and reassessed within the first 72 hours of presentation using EQ-5D-5L, EQ-VAS and the Integrated Palliative Outcomes Scale (IPOS).
Results
Completion rate was 68% (n = 151) and 33.1% were known to have died during admission or within 6 months post-discharge. The vast majority (84.8%) reported severe difficulties with at least one dimension of EQ-5D-5L at baseline and improvements in EQ-VAS observed at reassessment in 51.7%. The baseline IPOS revealed 78.2% of patients rating seven or more items as moderate, severe or overwhelming, but a significant reduction (-3.6, p < 0.001) in overall physical symptom severity and prevalence was also apparent. No significant differences were noted in emotional symptoms or changes in communication/practical issues. IPOS total score at follow up was positively associated with age, having comorbidities (Charlson index score > = 1) and negatively associated with baseline IPOS and CriSTAL scores.
Conclusion
Older people with poor prognosis admitted to hospital have very high symptom burden compared to population norms, though some improvement following assessment was observed on all measures. These data provide valuable descriptive information on quality of life among a priority population in practice and policy and can be used in future research to identify suitable interventions and model their effects.
Older adults at greater risk for Alzheimer’s disease show stronger associations between sleep apnea severity and verbal memory
Authors: Lui Kitty , Dave Abhishek , Sprecher Kate , Chappel-Farley Miranda , Riedner Brady , Heston Margo , Taylor Chase , Carlsson Cynthia , Okonkwo Ozioma , Asthana Sanjay , Johnson Sterling , Bendlin Barbara , Mander Bryce , Benca Ruth 
Published: December 2023

Abstract

Background: Obstructive sleep apnea (OSA) increases risk for cognitive decline and Alzheimer’s disease (AD). While the underlying mechanisms remain unclear, hypoxemia during OSA has been implicated in cognitive impairment. OSA during rapid eye movement (REM) sleep is usually more severe than in non-rapid eye movement (NREM) sleep, but the relative effect of oxyhemoglobin desaturation during REM versus NREM sleep on memory is not completely characterized. Here, we examined the impact of OSA, as well as the moderating effects of AD risk factors, on verbal memory in a sample of middle-aged and older adults with heightened AD risk.
Methods: Eighty-one adults (mean age:61.7±6.0 years, 62% females, 32% apolipoprotein E ε4 allele (APOE4) carriers, and 70% with parental history of AD) underwent clinical polysomnography including assessment of OSA. OSA features were derived in total, NREM, and REM sleep. REM-NREM ratios of OSA features were also calculated. Verbal memory was assessed with the Rey Auditory Verbal Learning Test (RAVLT). Multiple regression models evaluated the relationships between OSA features and RAVLT scores while adjusting for sex, age, time between assessments, education years, body mass index (BMI), and APOE4 status or parental history of AD. The significant main effects of OSA features on RAVLT performance and the moderating effects of AD risk factors (i.e., sex, age, APOE4 status, and parental history of AD) were examined.
Results: Apnea-hypopnea index (AHI), respiratory disturbance index (RDI), and oxyhemoglobin desaturation index (ODI) during REM sleep were negatively associated with RAVLT total learning and long-delay recall. Further, greater REM-NREM ratios of AHI, RDI, and ODI (i.e., more events in REM than NREM) were related to worse total learning and recall. We found specifically that the negative association between REM ODI and total learning was driven by adults 60+ years old. In addition, the negative relationships between REM-NREM ODI ratio and total learning and REM-NREM RDI ratio and long-delay recall were driven by APOE4 carriers.
Conclusion: Greater OSA severity, particularly during REM sleep, negatively affects verbal memory, especially for people with greater AD risk. These findings underscore the potential importance of proactive screening and treatment of REM OSA even if overall AHI appears low.
Prognostic and functional significance of SLC7A11 and its relationship with the proliferation and apoptosis of acute myeloid leukaemia cells
Authors: Li Jiazheng , Zheng Yongzhi , Lu Lihua , Liu Wanying , Yang Ting , Hu Jianda , Chen Yanxin 
Published: December 2023

Abstract
Purpose: Acute myeloid leukaemia (AML) is a common type of leukaemia in adults. Solute carrier family 7 member 11 (SLC7A11) may be a therapeutic target for multiple cancer types. However, the effects and action mechanism of SLC7A11 in AML remain unknown. Here, we investigated the prognostic and functional significance of SLC7A11 in AML.
Methods: We performed Gene Ontology/Kyoto Encyclopedia of Genes and Genomes (KEGG), protein–protein interaction network, immune cell infiltration, transcription factor-miRNA–mRNA regulatory network, mutation, and methylation analyses.
Results: SLC7A11 was significantly upregulated in most tumours, including AML, and correlated with a poor overall survival. A comparison of the high and low SLC7A11 expression groups revealed 1184 differentially expressed genes and 699 genes co-expressed with SLC7A11. The KEGG pathway enrichment analysis revealed their involvement in the Rap1 signalling pathway. The immune infiltration analysis indicated that SLC7A11 expression correlated with the infiltration levels of eosinophils and naïve B, plasma B, CD8+ T, CD4+ memory activated, resting and activated natural killer, and resting myeloid dendritic cells. The regulatory network indicated that GATA1 and has-miR-143-3pmay be upstream regulators of SLC7A11. The methylation levels of seven CpG sites were found to be associated with prognosis using the MethSurv database. In vitro experiments showed that erastin, an SLC7A11 inhibitor, inhibited cell proliferation, prolonged the G1 phase, and shortened the S phase, whilst daunorubicin had a synergistic effect on the inhibition of AML cell (U937 and THP1) proliferation.
Conclusion: These findings indicate that SLC7A11 is a potential therapeutic target for AML, which may be regulated by the GATA1- has-miR-143-3p -SLC7A11network and the RAP1 signaling pathway.
Curcumin’s molecular mechanism of action and targets in the treatment of rheumatoid arthritis: A network analysis and molecular docking study
Authors: Kalungi Frank , Kumar Pradeep , Valdez Ivan Ricardo Vega , Anywar Godwin 
Published: December 2023

Abstract
Introduction: Rheumatoid Arthritis (RA) is an autoimmune disorder that majorly affects the joints leading to pain, swelling, and stiffness and inflammation. Curcumin is a chemical compound from Curcuma longa(Tumeric). The aim of this study was to investigate the molecular mechanisms underlying the treatment of RA using curcumin.
Methods: Curcumin associated targets were retrieved from SwissTargetPrediction, PharmMapper and DrugBank. The RA associated targets were retrieved from OMIM, GeneCards, NCBI gene databases. GeneVenn was used to determine overlapping genes (RA-curcumin associated targets). The targets were used to construct a compound-disease target network. Gene Ontology enrichment analysis was done to identify the molecular function, cellular components and biological processes associated with the targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses was performed to identify top pathways with p< 0.05. A disease target-pathway network (D-P) was constructed and then merged with the C-D network to produce a compound-disease target-pathway network (C-D-P).
Results: We obtained 190 RA-curcumin associated targets.Gene ontology analysis revealed response to peptide, protein kinase complex and non-membrane spanning protein kinase activity as the major biological processes, cellular componentsand molecular functionterms respectively. Network analysis revealed SRC, AKT1 and AKT2 as the hub targets. Molecular docking showed that curcumin can bind stably to the hub targets.
Conclusion: Curcumin can interact with various proteins involved in the treatment of RA which can guide further its clinical application.
Laser microbeam neuron ablation: a new approach in investigation chemotaxis response of nematode
Authors: Salama Saly N. , Atta Diaa , Tawfik Walid , Mohamed Mohamed N. , Radwan Nahla A. 
Published: December 2023

Abstract
Chemotaxis is the biologically intrinsic navigation towards or away from chemical stimuli; it is a crucial behavioral response for animals when interacting with their environment. It facilitates the location of sustenance, hosts, and other vital environmental signals. While prior research has predominantly concentrated on the chemotactic behaviors of free-living and entomopathogenic nematodes in response to volatile soil constituents, the current investigation extends this understanding to parasitic nematodes. Specifically, we examine Syphacia obvelata, a member of the Oxyuridae (Nematoda) known for parasitizing the vertebrate cecum. Employing laser surgery, we selectively ablated the olfactory receptors (amphids) to ascertain the reliance of S.obvelata's chemotactic behavior solely on stimuli from these sensory structures. This research, conducted in Egypt, pioneers the adaptation of laser surgery for the targeted incapacitation of sensory receptors in parasitic nematodes. The successful ablation and subsequent behavioral alteration highlight laser microsurgery's ability to ablation minuscule receptors on a motile parasitic worm, promising a valuable tool for comprehending such intricate parasitic species behavior and offering new vistas for parasitological research and potential control strategies.
Incidence of referred progressive collapsing foot deformity in the Swedish general population
Authors: Osbeck Ida , Cöster Maria , Atroshi Isam 
Published: December 2023

Abstract
Background: Progressive collapsing foot deformity (PCFD) is a disabling condition that may require complex surgical treatment. Little is known about the incidence of PCFD in the general population and specifically of PCFD requiring specialist care.
Methods: We conducted a nation-wide epidemiological study to estimate the incidence of referred PCFD in the general population. We retrieved data from the Swedish National Patient Register. All individuals aged 16 years or older, with a first-time diagnosis of PCFD (ICD-10 code M214) between 2007 and 2018 were identified. Total incidences, change over time, gender- and age specific incidences per 100,000 person-years were calculated using population size data from Statistics Sweden. Incidences were compared using Poisson test.
Results: The incidence rate (IR) of referred PCFD in the general population was 23.0 (95% CI 22.7-23.3) per 100,000 person-years. The IR in women was 30.4 (95% CI 29.9-30.8) and in men 15.4 (95% CI 15.1-15.8). The highest IRs were found in the age group 61-75 years old. Among the 21 regions in Sweden, large variations in the IRs were found, with age- and sex-standardized IRs ranging from 8.3 (95% CI 7.2-9.4) to 69.1 (95% CI 62.4-75.8).
Conclusion: PCFD requiring specialist care is common in the general population. The incidence in women is almost double that in men. Large unexplained regional variations in the incidence rates exist.
Creating silver nanoparticles through the biogenic synthesis process using marine fungi Aspergillus flavus OQ651270: Examining their characterization and conducting biological studies.
Authors: Al-Agamy Mohamed H. , Kelany Mahmoud S. , Alhuzani Mohammad R. , Hamed Moaz M 
Published: December 2023

Abstract
Silver nanoparticles (Ag-NPs) have gained widespread attention due to their diverse applications in modern technology, prompting extensive research and development efforts. This study focuses on investigating the antibacterial properties of Ag-NPs, encompassing their production, characterization, and biological aspects. The eco-friendly extracellular biosynthetic method employed in this work utilized extracts from the marine fungus Aspergillus flavus MK4 as reducing agents for nanoparticle synthesis. UV-visible spectroscopy revealed the colloidal Ag-NPs, with a Plasmon absorbance peak observed at 450 nm. Transmission electron microscopy (TEM) elucidated the spherical shape and 15 nm size of the Ag-NPs. Evaluation against both Gram-negative and Gram-positive bacteria demonstrated significant antibacterial activity. Cytotoxicity against the HepG-2 cell line was assessed using the MTT assay, and antioxidant properties were examined through the radical scavenging (DPPH) assay. The synthesized Ag-NPs exhibit promising characteristics for applications in antibacterial, anticancer, and wound-healing contexts. Aspergillus flavus MK4, with its robust Ag-NP synthesis capability, produces well-characterized nanoparticles with proven antibacterial, antioxidant, anticancer, and wound-healing properties.
The P limitation of Pinus massoniana reforestation increases with stand maturity: Evidence from plants, leaf litter, and soil
Authors: Tie Liehua , Peñuelas Josep , Huang Congde , Sardans Jordi , Bose Arun K. , Ouyang Shengnan , Kong Yixian , Guo Yan , Wu Yujie , Cheng Wei , Ding Guijie 
Published: December 2023

Abstract
Background and Aims
: Soil phosphorus (P) regulates plant productivity. However, the medium to long-term time-scale effects of reforestation on the P recycle of plants, litter, and soil continuum, thus regulating soil P storage, especially deep-soil P fractions dynamics, are unclear.
Methods
We determined soil (0 ~ 100 cm) P fractions, root and leaf N/P ratio, leaf-litter P concentration, and biogeochemical drivers in 32-, 45-, and 60-year-old Pinus massoniana reforestations in southwest China.
Results
The storages of soil labile, moderately labile, and occluded P in the 45- and 60-year stands were lower than the 32-year stand. The concentrations of NaHCO3-Pi, NaHCO3-Po, and total labile P in top-soil were lower while those in sub- and bottom-soil were not significantly in the 45- and 60-year stands compared to the 32-year stand. The concentrations of all soil layer NaOH-Po and total moderately labile P were lower in the 45- and 60-year stands than the 32-year stand. The concentrations of all soil layers C.HCl-Pi and total occluded P were lower in the 60-year stand than the 32-year stand. The leaf N/P ratio and top-soil C/P ratio and acid phosphatase were higher and the leaf-litter P concentration was lower in the older stands.
Conclusions
P. massoniana secreted more acid phosphatase and increased leaf P resorption to compensate for the drop in soil P-availability with stand age, which in turn decreased leaf-litter P input and thus resulted in a depletion of soil P. Overall, our results highlighted that P limitation increased with stand maturity in P. massoniana reforestations.