bluuebunny/crossref_metadata_2025_split huggingface dataset:
Affordable optical clearing and immunolabelling in mouse brain slices
Authors: Muza Phillip M. , Pérez Marta , Noy Suzanna , Kurosawa Miyu , Katsouri Loukia , Tybulewicz Victor L. J. , Fisher Elizabeth M.C. , West Steven J. 
Published: September 2023

AbstractTraditional histological analysis is conducted on thin tissue sections, limiting the data capture from large tissue volumes to 2D profiles, and requiring stereological methods for 3D assessment. Recent advances in microscopical and tissue clearing methods have facilitated 3D reconstructions of tissue structure. However, staining of large tissue blocks remains a challenge, often requiring specialised and expensive equipment to clear and immunolabel tissue. Here, we present the Affordable Brain Slice Optical Clearing (ABSOC) method: a modified iDISCO protocol which enables clearing and immunolabeling of mouse brain slices up to 1 mm thick using inexpensive reagents and equipment, with no intensive expert training required. We illustrate the use of ABSOC in 1 mm C57BL/6J mouse coronal brain slices sectioned through the dorsal hippocampus and immunolabelled with an anti-calretinin antibody. The ABSOC method can be readily used for histological studies of mouse brain in order to move from the use of very thin tissue sections to large volumes of tissue – giving more representative analysis of biological samples, without the need for sampling of small regions only.
Prospective longitudinal associations between adverse childhood experiences and adult mental health outcomes: a protocol for a systematic review and meta-analysis
Authors: Thurston Christina , Murray Aja Louise , Franchino-Olsen Hannabeth , Meinck Franziska 
Published: September 2023

Abstract
Background
Research cites a strong, dose–response relationship between adverse childhood experiences (ACEs) and poor adult mental health outcomes including anxiety, depression, post-traumatic stress disorder (PTSD), self-harm, suicidality, and psychotic-like experiences.

Aim
To systematically investigate the existence and strength of association between ACEs and adult mental health outcomes in prospective longitudinal studies. The review will focus on the outcomes: anxiety, depression, PTSD, self-harm, suicidal ideation, and psychotic-like experiences.

Methods
Twelve electronic databases will be searched: Embase, PsycINFO, MEDLINE, and Global Health through the OVID interface. ProQuest will be used to search Public Affairs Information Service (PAIS), Dissertations and Theses, Sociology Database (including Sociological Abstracts and Social Services Abstracts), PTSDpubs (formerly The Published International Literature on Traumatic Stress (PILOTS) Database) and Applied Social Sciences Index and Abstracts (ASSIA). CINAHL, World Health Organisation (WHO) Global Index Medicus, and WHO Violence Info will also be searched. Eligible studies will be double screened, assessed, and their data will be extracted. Any disagreement throughout these processes will be settled by a third reviewer. If enough studies meet the criteria and the methodological quality of each study is sufficient, a meta-analysis will be conducted.

Analysis
A narrative synthesis of included studies and the associations between ACEs and adult mental health will be completed. If the number of studies included per mental health outcome is two or more, a multi-level meta-analysis will be completed using odds ratio effect sizes as outcomes.

Discussion
This review will contribute to the existing body of literature supporting the long-term effects of ACEs on adult mental health. This review adds to previous reviews that have either synthesised cross-sectional associations between ACEs and mental health outcomes, synthesised longitudinal studies exploring the effect of ACEs on different physical and mental health outcomes or synthesised longitudinal studies exploring the effect of ACEs on the same mental health outcomes using different methods. This review aims to identify methodological weaknesses and knowledge gaps in current literature that can be addressed in future primary studies.

Systematic review registration
This protocol has been registered in PROSPERO (CRD42021297882).
Identity development, attraction, and behaviour of heterosexually identified men who have sex with men: scoping review protocol
Authors: Eaton Andrew D. , Scheadler Travis R. , Bradley Cara , McInroy Lauren B. , Beer Oliver W. J. , Beckwell Erin , Busch Adam , Shuper Paul A. 
Published: September 2023

AbstractBackgroundHeterosexually identified men who have sex with men (H-MSM) are distinct from other heterosexual men and from gay, bisexual, and other sexual minority men. Specifically, H-MSM experience discordance between their sexual identity (i.e., heterosexual) and behaviours (i.e., sexual encounters with other men). This sexual identity-behaviour discordance can create barriers to obtaining healthcare and social support. Understanding and accepting H-MSM as they self-identify may be necessary to implement effective public health and psychosocial interventions. The aim of the present study is to provide an overview of research on H-MSM.MethodsA scoping review will be conducted to identify and describe the identity development, attraction, and behaviour of H-MSM. This scoping review will also identify and describe current trends related to the recruitment of H-MSM and recommend directions for future research. Searches will be conducted in Academic Search Complete, APA PsychInfo, CINAHL Plus with full text, Education Research Complete, Gender Studies Database, GenderWatch, Health Source: Nursing/Academic Edition, LGBTQ + Source, MEDLINE, Psychology and Behavioral Sciences Collection, SocINDEX with full text, Sociological Collection, Social Work Abstracts, ProQuest Dissertations and Theses, and ResearchGate. Primary research studies published in peer-reviewed journals will be included. Dissertations and theses that include primary research on H-MSM will also be included. Reference lists, experts in the field, preprint servers, and relevant conferences will also be consulted for extant and in-progress literature. Two reviewers will independently pilot the data extraction form and conduct the title and abstract screening, with consultation from a research librarian. Seven reviewers will then conduct the full-text article screening. Thematic content analysis will guide the review; through independent review and reviewer meetings, themes and subthemes will be identified and reported from the extracted literature.DiscussionThis is the first known knowledge synthesis on H-MSM, seeking to better understand sexual identity-behaviour discordance amongst cisgender men. We anticipate that a theoretical framework of H-MSM’s sexuality, internal processes, and behaviours will be constructed from this review. Alongside implications for further research with H-MSM, this review may be relevant to sexually transmitted infection public health and to clinicians working in the field of male sexuality.Systematic review registrationOpen Science Framework:https://doi.org/10.17605/OSF.IO/MVY9H
Lessons learned from housing first, rapid rehousing trials with youth experiencing homelessness
Authors: Slesnick Natasha , Brakenhoff Brittany , Bunger Alicia , Chavez Laura , Cuthbertson Caleb , Famelia Ruri , Feng Xin , Fitzpatrick Maggie , Ford Jodi , Hatsu Irene , Holowacz Eugene , Jaderlund Soren , Kelleher Kelly , Luthy Ellison , Mallory Allen , Martin Jared , Pizzulo Alexis , Stone-Sabali Steven , Yilmazer Tansel , Wu Qiong , Zhang Jing 
Published: September 2023

Abstract
Background
Youth, 18 to 24 years, experiencing homelessness (YEH) are recognized as having developmental challenges dissimilar to older adults. Yet, research on efforts to end homelessness and prevent or intervene in drug use and mental health problems among youth have lagged behind that of adults. The Housing First (HF) Model which underlies Permanent Supportive Housing (PSH) and Rapid Re-Housing (RRH) has become preferred over treatment-first models.

Methods and results
We provide an overview of PSH and RRH studies to date and summarize our current understanding of their utility for use with YEH. Finally, we review our team’s current and past randomized trials testing RRH with YEH, providing lessons learned and recommendations.

Conclusion
Current research efforts to guide best practices are hampered by a lack of fidelity to HF principles, lack of randomized design, and lack of focus on youth. Lessons learned and recommendations from our work are offered to facilitate the future work of those who seek to end homelessness and address drug use and mental health problems among youth.
Comparative analysis of alignment algorithms for macular optical coherence tomography imaging
Authors: Jones Craig K. , Li Bochong , Wu Jo-Hsuan , Nakaguchi Toshiya , Xuan Ping , Liu T. Y. Alvin 
Published: October 2023

Abstract
Background
Optical coherence tomography (OCT) is the most important and commonly utilized imaging modality in ophthalmology and is especially crucial for the diagnosis and management of macular diseases. Each OCT volume is typically only available as a series of cross-sectional images (B-scans) that are accessible through proprietary software programs which accompany the OCT machines. To maximize the potential of OCT imaging for machine learning purposes, each OCT image should be analyzed en bloc as a 3D volume, which requires aligning all the cross-sectional images within a particular volume.

Methods
A dataset of OCT B-scans obtained from 48 age-related macular degeneration (AMD) patients and 50 normal controls was used to evaluate five registration algorithms. After alignment of B-scans from each patient, an en face surface map was created to measure the registration quality, based on an automatically generated Laplace difference of the surface map–the smoother the surface map, the smaller the average Laplace difference. To demonstrate the usefulness of B-scan alignment, we trained a 3D convolutional neural network (CNN) to detect age-related macular degeneration (AMD) on OCT images and compared the performance of the model with and without B-scan alignment.

Results
The mean Laplace difference of the surface map before registration was 27 ± 4.2 pixels for the AMD group and 26.6 ± 4 pixels for the control group. After alignment, the smoothness of the surface map was improved, with a mean Laplace difference of 5.5 ± 2.7 pixels for Advanced Normalization Tools Symmetric image Normalization (ANTs-SyN) registration algorithm in the AMD group and a mean Laplace difference of 4.3 ± 1.4.2 pixels for ANTs in the control group. Our 3D CNN achieved superior performance in detecting AMD, when aligned OCT B-scans were used (AUC 0.95 aligned vs. 0.89 unaligned).

Conclusions
We introduced a novel metric to quantify OCT B-scan alignment and compared the effectiveness of five alignment algorithms. We confirmed that alignment could be improved in a statistically significant manner with readily available alignment algorithms that are available to the public, and the ANTs algorithm provided the most robust performance overall. We further demonstrated that alignment of OCT B-scans will likely be useful for training 3D CNN models.
The efficacy and safety of perioperative administration of dexamethasone: a systematic review and meta-analysis
Authors: Najafzadeh Mohammad Javad , Shafiei Mohadeseh , Sharifi Meraj , Nazari Parvaneh , Nasiri Naser , Hashemian Morteza 
Published: October 2023

Abstract
Background
Perioperative prophylactic dexamethasone is commonly administered to reduce postoperative nausea and vomiting (PONV) and pain after surgery. In this study, we aimed to systematically review the efficacy and adverse effects of perioperative dexamethasone administration.

Methods
We conducted a systematic search until January 2023 in scientific databases, including PubMed, Scopus, Embase, Web of Science, and Google Scholar. After assessing the methodological quality of relevant studies, we synthesized those focusing on PONV, oral food intake tolerance, impaired wound healing, major postoperative complications, and postoperative infections following the perioperative administration of dexamethasone.

Results
A total of 27 studies were included in this systematic review and meta-analysis. The dexamethasone group showed decreased PONV (OR = 0.19; 95% CI 0.06–0.55), increased oral food intake tolerance (OR = 7.38; 95% CI 1.07–51.11), increased risk of impaired wound healing (OR = .48; 95% CI 0.52–4.21), decreased probability of postoperative infection (OR = 0.61; 95% CI 0.51–0.72), and increased risk of major postoperative complications (OR = 1.27; 95% CI 0.68–2.39) compared to the controls.

Conclusions
The results of our pooled data analysis showed that dexamethasone was superior to the control in terms of PONV, oral food intake tolerance, and postoperative infections.
Eating After Acute Psychosocial Stress in Healthy Men and Women: Sex Differences and Endocrine Mechanisms
Authors: Degroote Cathy , Renner Britta , Wickl Julia , Leven Anika , Wirtz Petra H 
Published: September 2023

Abstract

Context
Overweight and obesity have become a major health burden with a higher prevalence of obesity in women than in men. Mental stress has been discussed to play a role in this context.

Objective
We investigated endocrine mechanisms underlying eating after acute psychosocial stress and potential sex differences therein.

Methods
A total of 32 male and 31 female healthy participants underwent the Trier Social Stress Test before they tasted ice cream in a bogus taste test 15 minutes after stress. We repeatedly assessed the stress hormone cortisol and the satiety hormone cholecystokinin (CCK) in saliva as well as perceived hunger before and up to 1 hour after stress.

Results
Lower immediate total cortisol stress reactivity predicted higher hunger (Ps ≤ .004), but was not associated with food intake (Ps ≥ .90) or total CCK release (Ps ≥ .84). As compared to men, women ate less after stress (Ps &lt; .001) and had consistently lower levels of hunger (Ps ≤ .024) and cortisol (Ps ≤ .008) as well as a lower immediate total cortisol stress reactivity (Ps = .002). Further, they differed in the kinetics of CCK over the total experimental procedure (Ps ≤ .011), in immediate reaction to stress (Ps ≤ .038), and after eating (Ps ≤ .072), with women’s CCK levels continuously decreasing while men’s CCK levels were reactive.

Conclusion
We found evidence for lower immediate total cortisol stress reactivity relating to higher perceived hunger, with lower cortisol levels in women. Unlike in men, CCK levels in women were not reactive to acute stress and eating and decreased continuously. Our results may suggest a higher risk for stress-induced eating in women.
Lesions of KNDy and Kiss1R Neurons in the Arcuate Nucleus Produce Different Effects on LH Pulse Patterns in Female Sheep
Authors: Goodman Robert L , Moore Aleisha M , Onslow Kayla , Hileman Stanley M , Hardy Steve L , Bowdridge Elizabeth C , Walters Burgundy A , Agus Sami , Griesgraber Max J , Aerts Eliana G , Lehman Michael N , Coolen Lique M 
Published: September 2023

Abstract
The current model for the synchronization of GnRH neural activity driving GnRH and LH pulses proposes that a set of arcuate (ARC) neurons that contain kisspeptin, neurokinin B, and dynorphin (KNDy neurons) is the GnRH pulse generator. This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates. Injection of NK3-SAP ablated over 90% of the KNDy cells, while Kiss-SAP (saporin conjugated to kisspeptin-54) lesioned about two-thirds of the Kiss1R population without affecting KNDy or GnRH cell number. Both lesions produced a dramatic decrease in LH pulse amplitude but had different effects on LH pulse patterns. NK3-SAP increased interpulse interval, but Kiss-SAP did not. In contrast, Kiss-SAP disrupted the regular hourly occurrence of LH pulses, but NK3-SAP did not. Because Kiss1R is not expressed in KNDy cells, HiPlex RNAScope was used to assess the colocalization of 8 neurotransmitters and 3 receptors in ARC Kiss1R-containing cells. Kiss1R cells primarily contained transcript markers for GABA (68%), glutamate (28%), ESR1 (estrogen receptor-α) mRNA, and OPRK1 (kappa opioid receptor) mRNA. These data support the conclusion that KNDy neurons are essential for GnRH pulses in ewes, whereas ARC Kiss1R cells are not but do maintain the amplitude and regularity of GnRH pulses. We thus propose that in sheep, ARC Kiss1R neurons form part of a positive feedback circuit that reinforces the activity of the KNDy neural network, with GABA or glutamate likely being involved.
Prevention as a Factor of Economic Sustainability of Workplace Accident Insurance Programs
Authors: Abikenova Sholpan K. , Nasyrova Gulmira A. , Shayakhmetova Aizhan G. , Sarybayeva Inara E. 
Published: October 2023

In the scientific article, the positive impact of preventive accident insurance programs in the workplace is studied. Such programs are the most common and strategically significant type of social security in many countries. Insurance programs often include medical services, vocational rehabilitation, and benefits for injured workers or family members of deceased breadwinners. However, considering current trends, preventive measures aimed at stimulating the improvement of working conditions and occupational safety, active support, and funding of informational and preventive measures remain relevant. The article provides an overview of the use of preventive measures in insurance programs and suggests improving their effectiveness in enhancing working conditions. Firstly, it is proposed to create a unified information database for monitoring insurance contracts, controlling insurance flows on an industry-specific basis, for each enterprise and specific profession, as well as supervising the completeness and quality of preventive measures and the timely provision of compensation to injured workers and rehabilitation measures. Secondly, within the framework of assessing insurance risk, it is suggested to implement comprehensive assessment of occupational risk through the insurance fund. The results of the occupational risk assessment will serve as the basis for applying a two-component insurance tariff, taking into account a surcharge if the working conditions are unsafe. Thirdly, it is proposed to provide occupational health and safety training through insurance financing.
Keywords: safe work, risk-oriented approach, insurance, industrial accident, prevention.
Truth in Criminal and Civil Proceedings: Ensuring Sustainable Development of Society and Social Peace
Authors: Kudeikina Inga , Kaija Sandra 
Published: October 2023

The right to a fair trial is a key element of the rule of law. It should be noted that the right to a fair trial has a complex structure, because securing this right is both a goal, or a state’s positive commitment to ensuring citizens’ rights, and a means of achieving this goal. The obligation to tell the truth as a principle of procedure constitutes the core of a fair trial. The concept of ‘fair trial’ would be a mere fiction if the state would not demand that parties to the case tell the truth in proceedings. There is no doubt that a dispute can be solved in reliance on true facts, true statements. This is a pre-requisite for the legal resolution of disputes, which means confidence in the judiciary as an element of state’s sustainability in the long run. The objective of the research is to examine the scope of the obligation to tell the truth in procedural relations in conjunction with the need for the fair settlement of legal relationships in the context of the sustainability of society in order to formulate suggestions for improving the legislation, thereby minimising opportunities to avoid the truth for the parties to proceedings.The research has employed descriptive and analytical, deductive and inductive methods. These methods have been used to analyse laws and the opinions of legal scholars and formulate conclusions and suggestions.
Keywords: civil proceedings, criminal proceedings, obligation to tell the truth
Additional 30-Second Observation of the Right-Sided Colon for Missed Polyp Detection With Texture and Color Enhancement Imaging Compared with Narrow Band Imaging: A Randomized Trial
Authors: Yoshida Naohisa , Inagaki Yoshikazu , Inada Yutaka , Kobayashi Reo , Tomita Yuri , Hashimoto Hikaru , Dohi Osamu , Hirose Ryohei , Inoue Ken , Murakami Takaaki , Morimoto Yasutaka , Okuyama Yusuke , Morinaga Yukiko , Itoh Yoshito 
Published: October 2023

INTRODUCTION:
The efficacy of texture and color enhancement imaging (TXI) in the novel light-emitting diode endoscopic system for polyp detection has not been examined. We aimed to evaluate the noninferiority of the additional 30-second (Add-30-s) observation of the right-sided colon (cecum/ascending colon) with TXI compared with narrow band imaging (NBI) for detecting missed polyps.

METHODS:
We enrolled 381 patients ≥40 years old who underwent colonoscopy from September 2021 to June 2022 in 3 institutions and randomly assigned them to either the TXI or NBI groups. The right-sided colon was first observed with white light imaging in both groups. Second, after reinsertion from hepatic flexure to the cecum, the right-sided colon was observed with Add-30-s observation of either TXI or NBI. The primary endpoint was to examine the noninferiority of TXI to NBI using the mean number of adenomas and sessile serrated lesions per patient. The secondary ones were to examine adenoma detection rate, adenoma and sessile serrated lesions detection rates, and polyp detection rates in both groups.

RESULTS:
The TXI and NBI groups consisted of 177 and 181 patients, respectively, and the noninferiorities of the mean number of adenomas and sessile serrated lesions per patients in the second observation were significant (TXI 0.29 [51/177] vs NBI 0.30 [54/181], P < 0.01). The change in adenoma detection rate, adenoma and sessile serrated lesions detection rate, and polyp detection rate for the right-sided colon between the TXI and NBI groups were not different (10.2%/10.5% [P = 0.81], 13.0%/12.7% [P = 0.71], and 15.3%/13.8% [P = 0.71]), respectively.

DISCUSSION:
Regarding Add-30-s observation of the right-sided colon, TXI was noninferior to NBI.
Toada, abrigo da poesia
Authors: Azevedo Kenedi Santos 
Published: October 2023

A relação e o afastamento entre música e poesia têm gerado discussões no decorrer dos séculos, principalmente quando se pensa que, na antiguidade clássica, a poesia não tinha a autonomia angariada na modernidade. Esse fato começa a ser retomado na contemporaneidade, quando ocorre novamente o flerte entre esses gêneros. Sendo assim, o intuito deste trabalho é verificar de que modo a poesia se infiltra nas toadas de Ronaldo Barbosa, por meio de técnicas de composição, temas desenvolvidos e discurso lírico, a ponto de se dizer que essas músicas são “poesias caboclas”, escritas num ato de “inspiração”. Para tanto, são acionados teóricos, filósofos e ensaístas que tratem dessa relação e aqueles que mostram a definição desses gêneros, dentre os quais Aristóteles, com a Poética clássica, Giuliana Ragusa e seu Lira grega: antologia de poesia arcaica, Carlos Reis e o ensaio O conhecimento da literatura, sem interditar outros pesquisadores e pensadores dessa temática.