bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entrepreneurship and misallocation in production network economies
Authors: Cavalcanti Tiago , Mendes Angelo , Pannella Pierluca 
Published: November 2023

AbstractThis paper investigates how sectoral linkages amplify or diminish misallocation at the intensive and extensive margins. Our analysis is based on a multisector general equilibrium model with input–output linkages, heterogeneous entrepreneurial abilities, and endogenous occupational choice. Distortions affect the intensive use of production inputs and they also impact the agents’ occupational decisions, misallocating the mass and type of entrepreneurs in different sectors of production. When the most distorted sectors are upstream (downstream), input–output linkages amplify (diminish) the loss from entreprenurial misallocation. We calibrate the model to the US and quantify the output losses from sectoral corporate taxes, decomposing the role of networks and the extensive margin decisions. We find that sectoral linkages quadruple the loss from the misallocation of entrepreneurs. We study an entry subsidy program, showing that it should target those sectors whose marginal entrepreneurs suffer larger profit losses, even if they are not necessarily the most distorted.
Arthroscopic biceps tenodesis using press-fit bony plug: a case series study
Authors: Gad Ahmed Mahmoud , Zawam Sherif Hamdy 
Published: November 2023

Abstract
Purpose
To assess the feasibility, operative time, clinical outcomes, possible complications, and failure rates of all-through arthroscopic biceps tenodesis using press-fit bony plug technique.

Methods
This prospective case series study involved 30 skeletally mature patients with long head of biceps pathology (tendinitis after failure of conservative treatment, subluxation, dislocation, or tendon tears). All patients were followed up for 24 months at least.

Results
Twenty-nine patients regained full shoulder and elbow range of motion; one case suffered from reflex sympathetic dystrophy. There was a significant improvement in the constant, ASES, and VAS scores when comparing the pre-operative and post-operative values. The average biceps strength was 96% compared to the opposite healthy side. No cases were complicated by neuro-vascular deficits or failure of the tenodesis.

Conclusion
Press-fit biceps tenodesis is safe and accessible with low economic demands. We recommend this technique to be used more often when addressing patients with long head of biceps pathologies.

Registration data
Registration number: N-1562023.
Registration date: June 2022 “Retrospectively registered”.
Training and external validation of pre-treatment FDG PET-CT-based models for outcome prediction in anal squamous cell carcinoma
Authors: Frood Russell , Mercer Joseph , Brown Peter , Appelt Ane , Mistry Hitesh , Kochhar Rohit , Scarsbrook Andrew 
Published: November 2023

Abstract
Objectives
The incidence of anal squamous cell carcinoma (ASCC) is increasing worldwide, with a significant proportion of patients treated with curative intent having recurrence. The ability to accurately predict progression-free survival (PFS) and overall survival (OS) would allow for development of personalised treatment strategies. The aim of the study was to train and external test radiomic/clinical feature derived time-to-event prediction models.

Methods
Consecutive patients with ASCC treated with curative intent at two large tertiary referral centres with baseline FDG PET-CT were included. Radiomic feature extraction was performed using LIFEx software on the pre-treatment PET-CT. Two distinct predictive models for PFS and OS were trained and tuned at each of the centres, with the best performing models externally tested on the other centres’ patient cohort.

Results
A total of 187 patients were included from centre 1 (mean age 61.6 ± 11.5 years, median follow up 30 months, PFS events = 57/187, OS events = 46/187) and 257 patients were included from centre 2 (mean age 62.6 ± 12.3 years, median follow up 35 months, PFS events = 70/257, OS events = 54/257). The best performing model for PFS and OS was achieved using a Cox regression model based on age and metabolic tumour volume (MTV) with a training c-index of 0.7 and an external testing c-index of 0.7 (standard error = 0.4).

Conclusions
A combination of patient age and MTV has been demonstrated using external validation to have the potential to predict OS and PFS in ASCC patients.

Clinical relevance statement
A Cox regression model using patients’ age and metabolic tumour volume showed good predictive potential for progression-free survival in external testing. The benefits of a previous radiomics model published by our group could not be confirmed on external testing.

Key Points
• A predictive model based on patient age and metabolic tumour volume showed potential to predict overall survival and progression-free survival and was validated on an external test cohort.
• The methodology used to create a predictive model from age and metabolic tumour volume was repeatable using external cohort data.
• The predictive ability of positron emission tomography-computed tomography–derived radiomic features diminished when the influence of metabolic tumour volume was accounted for.
Beyond femininity or masculinity: gender typologies and healthy eating in early adulthood
Authors: González-Pascual Juan Luis , Esteban-Gonzalo Sara , Veiga Óscar Luis , Esteban-Gonzalo Laura 
Published: November 2023

Abstract
Purpose
In the field of health sciences gender is often confused with biological sex (male/female) or reduced to a dichotomous classification (masculinity/femininity). The concepts of sex and gender interact with each other, but they are not equivalent. According to Sandra Bem four gender typologies can be established (androgynous, masculine, feminine and undifferentiated).
A relationship has been shown to exist between gender and health. Yet, there is little evidence as to the relationship between gender typologies and adherence to the Mediterranean diet. The aim of this research is to evaluate the association between Bem’s gender typologies and adherence to the Mediterranean diet.

Methods
Mediterranean Diet Adherence Screener (MEDAS) and Bem’s gender typologies were the main variables. Sex, age, socioeconomic status (SES), body mass index (BMI) and obesity were analyzed as covariates.

Results
Multilevel multivariate analysis showed that androgynous typology was associated with increased adherence to Mediterranean diet (β = 0.46 (SE 0.21), p = 0.033), adjusting by covariates, in a university population in Spain. Moreover, this was not the case with masculinity or femininity typologies.

Conclusion
Thus, the results of this study suggest (1) that androgynous typology is not only associated with better mental health but also with healthy/healthier lifestyles, and (2) the complexity of the relationship between sex-gender and health would advise researchers avoid dichotomies such as male/female or masculinity/femininity.
Does multiple gastric aspirate collection increase sensitivity of M. tuberculosis detection in children with pulmonary tuberculosis?
Authors: Venturini Elisabetta , Bortone Barbara , Cini Gianmaria , Venanzi Jacopo , Pellegrino Roberta , Bartolesi Anna Maria , Vaggelli Guendalina , Trapani Sandra , Indolfi Giuseppe , Bianchi Leila , Montagnani Carlotta , Chiappini Elena , Rossolini Gian Maria , Galli Luisa 
Published: November 2023

AbstractThis study aims to investigate the sensitivity of microscopy, culture and polymerase chain reaction on three gastric aspirates (GAs) in the microbiological confirmation of active pulmonary tuberculosis (TB) and to identify possible changes in sensitivity derived from the collection of a different number of aspirates. Children with clinical and radiological diagnoses of active pulmonary TB who underwent three GAs between March 2007 and June 2019 were retrospectively evaluated. Clinical, radiological, and microbiological data were collected. The sensitivity of microbiological tests on GAs was calculated. Moreover, differences in sensitivity according to age and radiological pattern were investigated. Overall, 156 children with active pulmonary TB were enrolled with a median age of 51.5 (IQR: 25.2–113.2) months. Microbiological investigations on the first GA showed a sensitivity of 34% (95%CI 26.7, 42), the cumulative sensitivity of first and second GAs was 40.4% (95%CI 32.7, 48.5) and of the three GAs was 47.4% (95%CI 39.8, 55.2). The collection of three GAs leads to an overall increase in sensitivity of the first GA by 13.4% (95%CI 2.8, 24.1%; p=0.014). Moreover, the increase in sensitivity was significantly higher in children ≤ 4 years of age and in those with uncomplicated TB (p=0.008).Conclusions: Performing a higher number of GAs increases the sensitivity of microbiological confirmation of active pulmonary TB, particularly in children ≤ 4 years and with an uncomplicated radiological pattern.

What is known:• The diagnosis of paediatric tuberculosis is a challenge for paediatricians• Despite their low sensitivity gastric aspirates represent the standard sample for microbiological confirmation of active pulmonary tuberculosis in children• Most international guidelines recommend performing three sequential gastric aspirates on three consecutive days

What is new:• A significant increase in global sensitivity by 13.4% was found by the collection of three gastric aspirates compared to the first one• Performing a higher number of gastric aspirates increases the sensitivity of microbiological confirmation, particularly in children ≤ 4 years and with an uncomplicated radiological pattern
Effect of exercise across the head and neck cancer continuum: a systematic review of randomized controlled trials
Authors: Avancini Alice , Borsati Anita , Belluomini Lorenzo , Giannarelli Diana , Nocini Riccardo , Insolda Jessica , Sposito Marco , Schena Federico , Milella Michele , Pilotto Sara 
Published: November 2023

Abstract
Purpose
This study aims to systematically explore the impact of physical exercise as supportive therapy for head and neck cancer.

Methods
A systematic search on PubMed/MEDLINE, Cochrane, and SPORTDiscus was conducted. Randomized controlled trials exploring the effects of a physical exercise intervention in comparison with usual care on outcomes in patients with head and neck cancer were selected. The RoB 2 tool was used to determine the study quality. The extracted data are reported as qualitative synthesis.

Results
Among the 527 records examined, nine studies were included. No trials investigating exercise as prehabilitation were found, whereas eight studies involving 452 patients with head and neck cancer were conducted during anticancer treatment. Most trials did not report improvements in body mass index or body composition, while 2/4 and 3/5 investigations found a significant increase in muscle strength and cardiorespiratory fitness, respectively. Regarding the patients’ reported outcomes, 4 out of 7 studies observed enhancements in some domains of quality of life, and two trials out of 3 detected an amelioration in fatigue following the exercise intervention. Analyzing the exercise programs, it seems that combining aerobic and resistance training could be more beneficial compared to a single type of full-body exercise in counteracting physical decline and controlling symptoms in the anticancer therapy phase. One trial has investigated the effect of resistance exercise on patients who had terminated the anticancer treatments, reporting significant improvements in lean mass, muscle strength, and quality of life.

Conclusion
Exercise may be a promising approach in patients with head and neck cancer. Future studies are needed to consolidate these results.
Accounting for forest fire risks: global insights for climate change mitigation
Authors: Chu Long , Grafton R. Quentin , Nelson Harry 
Published: November 2023

AbstractFire is an important risk in global forest loss and contributed 20% to 25% of the global anthropogenic greenhouse gas emissions between 1997 and 2016. Forest fire risks will increase with climate change in some locations, but existing estimates of the costs of using forests for climate mitigation do not yet fully account for these risks or how these risks change inter-temporally. To quantify the importance of forest fire risks, we undertook a global study of individual country fire risks, combining economic datasets and global remote sensing data from 2001 to 2020. Our estimates of forest fire risk premia better account for the risk of forest burning that would be additional to the risk-free and break-even price of credits or offsets to promote carbon sequestration and storage in forests. Our results show the following: (1) forest fire risk premia can be much larger than the historical forest area burned; (2) for some countries, forest fire risk premia have a large impact on the relative country-level break-even price of carbon credits or offsets; (3) a large spatial and inter-temporal heterogeneity of forest fires across countries between 2001 and 2020; and (4) the importance of properly incorporating forest fire risk premia into carbon credits/offset programs. As part of our analysis, and to emphasise the possible sub-national scale differences, our results highlight the heterogeneity in fire risk premia across 10 Canadian provinces.
Relationship between circadian rhythm-related genes and extracellular matrix: implications for sleep deprivation
Authors: Liu Xuejuan , Sun Jia , Ling Zexia , Dong Tong 
Published: November 2023

Abstract
Background
Sleep deprivation (SD) can lead to the development of various pathological disorders. The extracellular matrix (ECM) compositions and circadian rhythm genes are two pivotal variables of SD. However, their relationships remain undefined during SD.

Methods
A mouse SD model was established using a modified multiplatform water environment method. The expression of nerve growth factor (NGF) in mouse hippocampus was detected by an immunofluorescence (IF) method. Protein expression was assessed by western blot, and mRNA analysis was performed by quantitative real-time PCR (qRT-PCR). The differentially expressed genes after SD, the genes associated with stromal score, and gene expression correlation were analyzed by bioinformatic analysis.

Results
The mouse model of SD was successfully established, as evidenced by the changed morphology, increased Bax and NGF levels, and downregulated Bcl-2 in mouse hippocampus after SD. The differentially expressed genes after SD were closely associated with the ECM compositions. The ECM composition metalloproteinase 9 (MMP9) was under-expressed in mouse hippocampus after SD. The hippocampal MMP9 expression was correlated with the expression levels of circadian genes PER2, PER3, TIMELESS, FBXL3, and NFIL3. PER2 and TIMELESS were upregulated in mouse hippocampus after SD.

Conclusion
The current findings suggest a correlation between ECM composition MMP9 and circadian rhythm-related genes PER2 and TIMELESS in mouse hippocampus after SD, providing a novel understanding of the disorders after SD.
SIGAA: signaling automated analysis: a new tool for Ca2+ signaling quantification using ratiometric Ca2+ dyes
Authors: Lopes Rafael Faria , Gonçalves-Ribeiro Joana , Sebastião Ana M. , Meneses Carlos , Vaz Sandra H. 
Published: November 2023

AbstractAstrocytes are non-neural cells, restricted to the brain and spinal cord, whose functions and morphology depend on their location. Astrocyte–astrocyte and astrocyte–neuron interactions occur through cytoplasmic Ca2+ level changes that are assessed to determine cell function and response (i.e., drug testing). The evaluation of alterations in intracellular Ca2+ levels primarily relies on fluorescence imaging techniques, performed through video recording of cells incubated with Ca2+-sensitive dyes. By observing ion concentration shifts over time in a delimited region of interest (ROI) encompassing a single cell, it is possible to draw conclusions on cell responses to specific stimuli. Our work describes a tool named SIGAA—signaling automated analysis, for astrocyte ROI-based fluorescent imaging. This tool is specifically tailored for two wavelengths excited dyes by using two inputs of Ca2+ signaling recorded frames/videos and outputting a set of features relevant to the experiment’s conclusions and cell characterization. SIGAA performs automatic drift correction for the two recorded videos with a template matching algorithm, followed by astrocyte identification (ROI) using morphological reconstruction techniques. Subsequently, SIGAA extracts intracellular Ca2+ evolution functions for all identified ROIs detects function transients, and estimates a set of features for each signal. These features closely resemble those obtained through traditional methods and software used thus far. SIGAA is a new fully automated tool, which can speed up hour-long studies and analysis to a few minutes, showing reliable results as the validity tests indicate.
Numerical modeling of vadose zone electrical resistivity to evaluate its hydraulic parameters
Authors: Masria Ali , Seif Ahmed Khaled , Ghareeb Mohamed , Abd-Elmaboud Mahmoud E. , Soliman Karim , Ammar A. I. 
Published: November 2023

AbstractStudying and determining the physical properties and hydraulic parameters of vadose zone sediments is an important key to evaluate the infiltration rate into them and assessing the extent to which aquifer sediments benefit from rainwater harvesting in arid and semi-arid areas. Due to the lack of sufficient data on the characteristics of this zone depths, a numerical modeling was used to simulate the electrical resistivity of these sediments by applying the electrical resistivity method, because it is the most affected by the physical properties of dry and wet sediments. This study was applied as a proposal for application in northwestern KSA to calculate the vertical hydraulic conductivity and transmissivity for the vadose zone. This was implemented by assuming a three-layer model using COMSOL Multiphysics model with different electrical resistivity values depending on some in situ electrical resistivity measurements for shallow depths. Hence, the infiltration rate of sediments in this area can be predicted with depth and its effect on aquifer recharge. The focus was on calculating the vertical hydraulic parameters of the most widespread surface sediments with depth and comparing the results of calculating these parameters for some sediments laboratory-wise to ensure their accuracy. Then, their infiltration rate was inferred separately with depth, predicting their ability to aquifer recharge and make the most of rainwater harvesting. Finally, this study can be considered as a preliminary study to determine the expected forward model of electrical resistivity and hydraulic parameters values for the vadose zone sediments with depth along the area and in any other areas, and then apply them accurately in situ to estimate the extent of its usefulness in rainwater harvesting, especially aquifer recharge.
Spatiotemporal trends of urban-induced land use and land cover change and implications on catchment surface imperviousness
Authors: Andualem Tesfa Gebrie , Peters Stefan , Hewa Guna A. , Boland John , Myers Baden R. 
Published: November 2023

AbstractUrbanization, changes in land use and land cover (LULC), and an increase in population collectively have significant impacts on urban catchments. However, a vast majority of LULC studies have been conducted using readily available satellite imagery, which often presents limitations due to its coarse spatial resolution. Such imagery fails to accurately depict the surface characteristics and diverse spectrum of LULC classifications contained within a single pixel. This study focused on the highly urbanized Dry Creek catchment in Adelaide, South Australia and aimed to determine the impact of urbanization on spatiotemporal changes in LULC and its implications for the land surface condition of the catchment. Very high spatial resolution imagery was utilized to examine changes in LULC over the past four decades. Support Vector Machine-learning-based image classification was utilized to classify and identify the changes in LULC over the study area. The classification accuracy showed strong agreement, with a kappa value greater than 0.8. The findings of this analysis showed that extensive urban development, which expanded the built-up area by 34 km2, were responsible for the decline in grass cover by 43.1 km2over the last 40 years (1979–2019). Moreover, built-up areas, plantation, and water features, in contrast to grass cover, have demonstrated an increasing trend during the study period. The overall urban expansion over the study period was 136.6%. Urbanization intensified impervious area coverage, increasing the runoff coefficient, equivalent impervious area, and curve number by 60.6%, 60.6%, and 7.9%, respectively, while decreasing the retention capacity by 38.6%. These modifications suggest a potential variability in catchment surface runoff, prompting the need for further research to understand the surface runoff changes brought by the changes in LULC resulting from urbanization. The findings of this study can be used for land use planning and flood management.
Cancer/testis-45A1 promotes cervical cancer cell tumorigenesis and drug resistance by activating oncogenic SRC and downstream signaling pathways
Authors: Meng Mei , Guo Yan , Chen Yu , Li Xu , Zhang Bin , Xie Zhijia , Liu Juntao , Zhao Zhe , Liu Yuxi , Zhang Tong , Qiao Yingnan , Shang Bingxue , Zhou Quansheng 
Published: November 2023

Abstract
Background
Cancer/testis antigen-45A1 (CT45A1) is overexpressed in various types of cancer but is not expressed in healthy women. The role of CT45A1 in cervical cancer has not yet been described in the literature.

Purpose
The aim of this research was to study the role of CT45A1 in cervical cancer progression and drug resistance, elucidate the mechanisms underlying CT45A1-mediated tumorigenesis and investigate CT45A1 as a biomarker for cervical cancer diagnosis, prognostic prediction, and targeted therapy.

Methods
The CT45A1 levels in the tumors from cervical cancer patients were measured using immunohistochemical staining. The role and mechanisms underlying CT45A1-mediated cervical cancer cell tumor growth, invasion, and drug resistance were studied using xenograft mice, cervical cancer cells, immunohistochemistry, RNA-seq, real-time qPCR, Chromatin immunoprecipitation and Western blotting.

Results
CT45A1 levels were notably high in the tumor tissues of human cervical cancer patients compared to the paracancerous tissues (p < 0.001). Overexpression of CT45A1 was closely associated with poor prognosis in cervical cancer patients. CT45A1 promoted cervical cancer cell tumor growth, invasion, neovascularization, and drug resistance. Mechanistically, CT45A1 promoted the expression of 128 pro-tumorigenic genes and concurrently activated key signaling pathways, including the oncogenic SRC, ERK, CREB, and YAP/TAZ signaling pathways. Furthermore, CT45A1-mediated tumorigenesis and drug resistance were markedly inhibited by the small molecule lycorine.

Conclusion
CT45A1 promotes cervical cancer cell tumorigenesis, neovascularization, and drug resistance by activating oncogenic SRC and downstream tumorigenic signaling pathways. These findings provide new insight into the pathogenesis of cervical cancer and offer a new platform for the development of novel therapeutics against cervical cancer.