bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inadvertent Detection of Rift Valley Fever Virus by Metagenomic Sequencing in Febrile Patients Seeking Health Care at Marigat Sub-District Hospital, Baringo County, Kenya
Authors: Lemtudo Allan , Kimita Gathii , Awinda George , Mutai Beth , Waitumbi John 
Published: December 2023

Abstract
Background
Rift Valley Fever (RVF) outbreaks occur following unusually heavy rainfall and flooding, which increase the breeding activities of mosquitoes that transmit the RVF virus (RVFV). Such conditions occurred from May to June 2018 in Wajir and Marsabit counties, northeastern Kenya. In December 2019, a blood sample that had been collected in June 2018 from a febrile child attending a hospital 648 km away from Wajir town produced a few short sequences that mapped to RVFV by shotgun metagenomic next-generation sequencing (mNGS). The agnostic mNGS was part of a pathogen discovery exercise that aimed to identify viral pathogens of concern that are missed by targeted testing for endemic fever-causing.
Methodology
Following identification of sequence reads that mapped to RVFV, we expanded subsequent testing by reverse transcriptase real time PCR (RT-qPCR) for RVFV to 44 serum samples, including the test case, collected between June and September 2018. Positive samples were further analyzed by shotgun metagenomics using a pathogen agnostic discovery pipeline that involves converting the RNA to cDNA, followed by random amplification using viral genome sequencing primers. The generated products were used for library preparation and subsequently sequenced on Illumina MiSeq. Genome assembly was performed using the ngs_mapper pipeline, while lineage classification and phylogeny were performed using rvfv typing tool v1 and phyml v3, respectively.
Results
Of the 44 specimens, three, including the index sample tested positive at cycle threshold (Ct) values of 19.3, 34.3 and 31 (index sample). The whole genome of RVFV comprising the large (L), medium (M), and small (S) segments was obtained from the serum sample with the lowest Ct value. The other samples had partial sequences of the L segment. The genomes were classified as C lineage. Phylogeny drawn from the whole genome sample clustered in a clade comprising sequences obtained from the 2017 human RVFV outbreak in Uganda and the 2021 cattle outbreak in Kiambu, Central Kenya.
Conclusion
This study used unbiased pathogen detection to identify presence of RVFV in the community living in Baringo County, Kenya that would otherwise have gone undocumented. Based on these data, RT-qPCR test for RVFV has been included in our routine testing panel for febrile illness.
LASIK is associated with early cataract surgery in healthy patients
Authors: Ortiz-Morales Gustavo , Ramos-Dávila Eugenia M. , Elizondo-Fernández Bárbara , Arreola-Martinez Jesus , Nava-Garcia Jose , Valdez-Garcia Jorge E. 
Published: December 2023

Abstract
Purpose: To evaluate the association between LASIK and early cataract phacoemulsification surgery (PE).
Methods: A matched case-control study was conducted. Cases were otherwise healthy adults with a history of LASIK. Groups were paired according to corrected-distance visual acuity (CDVA), axial length, and cataract grade.
Results: A total of 213 patients were included. 85 patients were classified as post-LASIK group and 128 as controls. The mean age at the time of LASIK was 42.32 ± 9.24 years. The mean CDVA before PE was 0.29 ± 0.19 Log MAR in post-LASIK group and 0.34 ± 0.22 Log MAR in controls (p=0.07). The mean axial length was 23.99 ± 1.78 mm in post-LASIK group and 23.62 ± 0.98 mm in controls (p=0.085). The mean nuclear cataract grading was 1.36 in post-LASIK group and 1.47 in controls (p=0.34). The mean age at the time of PE was 60.18 ± 7.46 years in post-LASIK group and 67.35 ± 9.28 in controls (p<0.0005). The difference between the mean age of LASIK and the mean age of PE was 17.85 ± 5.72 years. There was a positive association between the post-LASIK group and the age of PE ≤ 55 years (OR: 4.917, 95% CI: 2.21-10.90, p<0.001).
Conclusion: LASIK may be associated with early PE. Patients with LASIK had a 7-year earlier PE compared to a matched control group.
Spinal adhesive arachnoiditis in an adult patient with spinal muscular atrophy type 3 treated with intrathecal therapy
Authors: Ubysz Jakub , Koszewicz Magdalena , Bladowska Joanna , Budrewicz Slawomir 
Published: December 2023

Abstract
Background: Spinal adhesive arachoiditis is a chronic inflammatory process of the leptomeninges and intrathecal neural elements. The possible causes of arachnoiditis are: infections, injuries of spinal cord, surgical procedures and intrathecal administration of therapeutic substances or contrast.
Case presentation: We present a case of 56-old woman with spinal muscular atrophy type 3 who developed a severe back pain in the lumbosacral region after the fifth dose of nusinersen given intratecally. Magnetic resonance of lumbosacral spine showed spinal adhesive arachoiditis. She received high doses of methylprednisolone intravenously, and later non-steroidal anti-inflammatory drugs, alpha lipoic acid, vitamins and rehabilitation with slight improvement.
Conclusions: The authors summarize that scheduled resonance imaging of the lumbosacral spine may be an important element of the algorithm in the monitoring of novel, intrathecal therapy in patients with spinal muscular atrophy.
Comprehensive Analysis and Quality Assessment of Freeze Dried Methylene Diphosphonate Labelled with 99m-Technetium: An Extensive Comparison of Biodistribution Study with Commercial Standards
Authors: Tasnim Kifyat , Akhter Rabeya , Rahman Mustafizur , Hossain Anwar , Waheed Momtaz Fatima , Jahura Fatema Tuj , Reza Sultanur , Chowdhury Debasish , Amin Ruhul , Islam Tariqul , Haque Azizul , Uddin Jashim 
Published: December 2023

Abstract

Objective:Technetium-99m (99mTc) is an important radionuclide used for the prognosis of bone scintigraphy labelled with a suitable kit.
Methodology: The present investigations focus on the aseptic preparation and application of the methylene di-phosphonic acid (MDP) kit on bio-organ after labeling with 99mTc collected from a 99Mo/99mTc generator.
Result:Before going to bio-application of the labelled kit, radiochemical purity was determined by paper chromatography using the High Purity Germanium (HPGe) detector and the radiochemical purity of 99mTc-MDP was found to be 99%. Furthermore, negative results were found for pyrogen and bacterial endotoxin tests; fungal growth was also absent. Radiation dose uptake in different organs of Wister rats was measured by a dose calibrator, and observed results indicate the highest accumulation of 99mTc-MDP in the skull and skeletal part. This study also performed a comparative study model between laboratory and commercially prepared Monrol MDP kits. This study was performed as bio-application of laboratory prepared 99mTc-MDP prior to commercial production of cold kit at radioisotope production division of Bangladesh Atomic Energy Commission.
Conclusion: Overall, the observations from this study are within the accepted range and provide prospect for commercial production of the kits for human administration.
Population Pharmacokinetics of Pegasparaginase in pediatric ALL patients: Age as a significant covariate
Authors: Patil Anand , Krishnamurthy Manjunath Nookala , Narula Gaurav , Banavali Shripad D , Gota Vikram 
Published: December 2023

Abstract

Purpose
Pegasparaginase is essential in treating acute lymphoblastic leukemia (ALL). However, there is considerable variability in its pharmacokinetics, affecting the safety and efficacy of this drug. We aimed to develop a population pharmacokinetic (PopPK) model of pegasparaginase to identify covariates that might help to explain this variability. Quantitative assessments of the pharmacokinetics, efficacy, and safety of pegasparaginase in this patient population would help to optimize the dosing regimen.
Methods
PopPK analysis of pharmacokinetic data obtained from 29 pediatric ALL patients was accomplished using Phoenix® NLME software. The effect of various covariates (age, BSA, liver function parameters, etc.) on the pharmacokinetics of pegasparaginase was evaluated. The impact of the identified covariates on the safety and efficacy of pegasparaginase parameters was also assessed.
Results
A one-compartment model with first-order absorption and elimination adequately described the pegasparaginase pharmacokinetics. The patient's age was a clinically significant covariate for pegasparaginase disposition. Cmax in older age group children (> 120 months) was significantly low, resulting in overall lower exposure (AUC) when compared with the younger age group (≤ 120 months). Modifying the dose according to the formula (4* age in months + 715) IU/m2 BSA, predicted to achieve similar exposure across all the age groups in pediatric ALL patients.
Conclusions
The developed population pharmacokinetic model helped to establish age as a significant covariate for pegasparaginase pharmacokinetics, suggesting age-based dosing over and above the current BSA-based dosing practices could achieve uniform exposure in all age groups.
Examining the Relationship between Self-Perceptions of Aging, Psychological Well-Being and Health-Promoting Lifestyle: A Cross-Sectional Study in Iran
Authors: Nosrati Parisa , Momeni Khodamorad , Wahl Hans-Werner 
Published: December 2023

Abstract

Background:
We expected to replicate in this study conducted in Iran the established associations among more positive self-perceptions of aging (SPA) and better developmental outcomes in an Iranian sample. As extension of previous SPA research, we tested the inter-relations among different SPA constructs and outcomes simultaneously by means of structural equations model (SEM).
Methods:
The design was cross-sectional and included older adults 60 years and older living in the Iranian province of Kermanshah. A total of 347 individuals (169 men; 178 women) were recruited by means of convenience sampling. SPA indicators included three established scales (aging perceptions, attitudes toward aging, awareness of age-related change), all previously introduced in Iran. Outcomes included established measures of psychological well-being and health-promoting lifestyle, both also previously established in the Iran.
Results:
As expected, more positive SPA were positively associated with both developmental outcomes consistently across all three indicators. In SEM testing, defining awareness of age-related change as a mediator among aging perceptions, attitudes toward aging, and developmental outcomes achieved an acceptable fit, but was different depending on the SPA indicator: Inter-relations of aging perception and both outcomes were mediated only through AARC-Loss, whereas inter-relations among attitudes toward aging and both outcomes were mediated only through AARC-Gain. Further, AARC-Loss was a stronger mediating variable than AARC-Gain.
Conclusions:
Findings replicated the association between various SPA indicators and well-being and health related outcomes in an Iranian sample, thus extending the robustness of this relation across countries/cultures. We also confirmed the need for simultaneous analysis of different indicators of SPA and outcomes.
Development, validation, and updating of prognostic models for m7G- associated genes in lower-grade gliomas
Authors: Li Huijun , Sun Hao , Geng Ruirui , Shen Junjie , Dong Yongfei , Tang Zaixiang , Shi Lei , Lu Ke 
Published: December 2023

Abstract
Background
Studies are aiming at developing prognostic models using N7-methylguanosine (m7G)-related genes in gliomas, however, models with good predictive performance for lower-grade gliomas have yet to be developed.
Methods
Based on genes with m7G variants and clinical information, two prediction models have been derived to predict the probability of survival for patients with lower-grade gliomas in TCGA. The models were externally validated using independent datasets. Based on CGGA information, updated models that were created matched the features of the local population.
Results
Two models were derived, validated and updated. Model 1, which was derived on the basis of mRNA, only contains five genes: CD37, EIF3A, CALU, COLGALT1, and DDX3X. Model 2 included six variables: grade, age, gender, IDH mutation status, 1p/19q codeletion status and prognostic index of model 1. The C-statistic of revised model 1 was 0.764 (95%CI: 0.730–0.798) in the revised set and 0.700 (95%CI: 0.658–0.742) in the test set. Regarding internal validation, C-statistic for model 2 with 1000 bootstrap replications was 0.848, while in external validation, the C-statistic was 0.752 (95%CI: 0.714–0.788). Both models exhibited satisfactory calibration after updating in external validation. The models' web calculator is provided at https://lhj0520.shinyapps.io/M7G-LGG_model/.
Conclusion
we developed and validated two models and updated them, which makes the models better predictors for patients.
Single cells and TRUST4 reveal immunological features of the HFRS transcriptome
Authors: Xiao Ran , Lin Mu , liu Hao , Gong Yadong , Liu Mubo , Wu Deijing , Ma Qingqing 
Published: December 2023

Abstract
The pathogenesis of hemorrhagic fever with renal syndrome (HFRS) is greatly affected by different immune cells. At present, peripheral blood T cell receptor (TCR) or B cell receptor (BCR) library sequencing of HFRS is still lacking and expensive. In this study, the computational method TRUST4 was used to construct TCR and BCR libraries from a large number of RNA-seq data of peripheral blood of HFRS patients, and to analyze the clonality and diversity of disease immune libraries. Although the function of immune cells has been studied, the mechanism remains complex. The differentially expressed genes in each immune cell type and the cell-to-cell interactions between immune cell clusters have not been covered. In this work, we clustered 11 cell subsets from raw scRNA-seq data and disaggregated the characteristic changes in the proportion of cell subsets under disease conditions. CellChat, a cell-cell communication analysis tool, was also used to analyze the effects of MIF family, CD70 family, and GALECTIN family cytokines, which are reported to be involved in cell communication subsets, respectively. HDWGCNA analysis identified core genes involved in the occurrence and development of HFRS in T cells and B cells. The results of trajectory analysis showed that most cell subsets were in the developmental stage, transcription factors in Effector CD8+ T cells (GZMH), Effector CD8+ T cells (GZMK), It was highly expressed in Naive CD4+ T cells and Naive B cells. Our results comprehensively illustrated the dynamic changes of immune cells during the pathogenesis of HFRS. This work identifies specific V genes and J genes in TCR and BCR that can be used to extend our understanding of HFRS. These findings provide new insights into the diagnosis and treatment of this autoimmune disease.
An Empirical Investigation of Wagner's Law for Twelve Indian States: A Pooled Mean Group Approach Using Linear and Non-Linear Specifications
Authors: Choudhary Prakash , Chary Shreesh , Singh Namah 
Published: December 2023

Abstract
Wagner's Law, which is considered the first model of public spending in public finance history, suggests that there is a tendency for government spending to increase relative to national income over the long run. This study aims to empirically test the validity of Wagner's hypothesis for twelve Indian states grouped into high-debt and low-debt states, from 2002 to 2020 using a pooled mean group approach. The study also examines the short-run causality between government expenditure and per capita GDP using the Dumitrescu Hurlin Granger causality test. We found a long run relationship between state expenditure and per capita gross state domestic product. Furthermore, there appears to be a significant asymmetric effect of per capita GDP on the expenditure variables, for the entire sample. The study offers value to policy-makers in understanding expenditure dynamics of Indian states and the validity of Wagner’s law in low and high debt states.
Method for Establishing the Multipath Adaptive Mitigation Model Considering Deformation Displacement of Monitoring Stations
Authors: Guo Zihuai , Yao Yibin , Xu Chaoqian , Pan Peifen , Hu Mingxian 
Published: December 2023

Abstract
Multipath effects are a significant error source affecting the Global Navigation Satellite System (GNSS) positioning, and their impact cannot be overlooked in millimeter-level positioning applications such as deformation monitoring. Existing methods for multipath mitigation almost universally assume that a GNSS antenna does not undergo displacement. However, in deformation monitoring, the frequent displacements of monitoring points render these methods inapplicable. Therefore, it is imperative to study multipath modeling and mitigation in scenarios where monitoring points are displaced. To address this issue, this study introduces a multipath adaptive mitigation model (MAMM) that investigates the impact of monitoring point displacement on the effectiveness of multipath mitigation in different areas of the hemisphere model. It uses the multipath mitigation values of the normal grid as a constraint to re-estimate the real-time multipath mitigation values for abnormal grid areas, and to design experiments for validation. This study demonstrates that under centimeter-level antenna displacement conditions, the conventional multipath hemisphere model (MHM) shows a significant decrease in positioning accuracy improvement. The areas of abnormal multipath effect variation comprise no more than 20% of the total space, providing theoretical feasibility for the modeling process of this study. Comparisons of positioning accuracy show that, under a 10 mm displacement, the MHM improves positioning accuracy in the east, north, and elevation by 86.4%, 127.2%, and 131.8%, respectively, compared to the original positioning accuracy, whereas the MAMM achieves 115.8%, 162.5%, and 139.1%, respectively, which is comparable to the accuracy of MHM under non-displacement conditions. Under displacements exceeding 50 mm, the MHM's accuracy improvements were 48.1%, 71.1%, and 59.8%, respectively, whereas the MAMM showed 100%, 124.1%, and 107.5%, respectively, significantly outperforming the MHM. Considering that the deformation rates of existing displacement monitoring bodies are generally lower than 10 mm/day, the MAMM demonstrates its effectiveness in reducing multipath errors without the need for repeated modeling, thereby proving its substantial practical value.
Are multi-element combinations of natural soundscapes better for psychophysiological restoration than single-element natural soundscapes? --Evidence from a study in Zhangjiajie National Forest Park
Authors: Bai Zhengkang , Zhang Shuangquan 
Published: December 2023

Abstract

Most of the current soundscape research content is limited to the discussion of the restoration effect of single-element soundscapes, but it is the combination of sounds that is common in outdoor activities, and there is no evidence that the restoration of natural soundscapes is better with multi-element combinations. In this study, the Zhangjiajie National Forest Park in China was used as the research object, and the physiological indices of the subjects were collected through EEG signals, and the POMS short-form psychological scale was used to understand the subjective psychological responses of the subjects to the soundscape. The results showed that (1) The psychophysiological restorative ability of the natural soundscape of the National Forest Park was confirmed, and the subjects' psychological and physiological indices changed significantly and positively after listening to each section of the natural soundscape (p = 0.001). (2) The restorative effect of the multi-natural sound combination was ranked first in the overall ranking of the five natural soundscapes, and the multi-natural sound combination did indeed provide better restorative effects than the single-element sounds. (3) Gender does not usually have a significant effect on the restoration effect, and only Windy Sound among the four single-element nature sound landscapes and one multi-element combination of nature sound landscapes showed a significant gender difference, so in general, the effect of gender on the restoration effect of nature sound landscapes is not significant. In terms of research methodology, this study used cluster analysis to cluster the five types of natural soundscapes according to psychological and physiological recovery ability, and used ridge regression to construct mathematical models of the psychological and physiological recovery of each of the four natural soundscapes. The study of human physiological and psychological recovery from different types of natural soundscapes in China's national forest parks will provide a basis for soundscape planning, design, and policy formulation in national forest parks.
Rhizopogon luteolusand Ganoderma adspersum extracts inhibit invasion through the crosstalk antioxidant activity and apoptosis induced by pAKT/Rb
Authors: Demiray Aydın , Karagür Ege Rıza , Tel-Çayan Gülsen , Tokgün Onur , Akça Hakan , Duru Mehmet Emin 
Published: December 2023

Abstract
Objective: Lung cancer is the primary cause of cancer-related deaths globally, and in NSCLC cancers, kinase protein B (AKT) is associated with pathways such as proliferative, invasion and apoptosis. In this study, the relationship of mushroom extracts with these pathways was investigated. Mushrooms have a long history of use in traditional medicine to treat a variety of illnesses, and scientists have been exploring the potential use of their antioxidant and anticancer properties in modern medicine, a topic to which this paper contributes.
Materials and methods: Mushroom extracts were collected in 2022 and the other phases of the experiment were completed in 2023.We studied the lung cancer cell lines h1299, pc-3 and pc-14. Extracts of Rhizopogon luteolus and Ganoderma adspersum were applied to these to determine the IC50 values. Then, proliferative and invasion effects, apoptosis and antioxidant effects were investigated. Finally, Western blotting was performed to investigate the pathways of these effects.
Results: Rhizopogon luteolus and Ganoderma adspersum extracts have anti-proliferative and anti-invasive effects on lung cancer cell lines and induce apoptosis, which increases the antioxidant effect. The mushroom extracts work through the p-AKT and Rb pathways.
Conclusions: We anticipate that Rhizopogon luteolus and Ganoderma adspersum extracts will make effective additions to cancer treatment as agents that work to suppress lung cancer cells via the p-Akt and Rb pathways.
A Highly Tunable Ultra-Low Cut-Off Frequency gm-c Filter with Bandwidth Stabilization Technique
Authors: Sasan Morteza Mahdi , Baghtash Hasan Faraji 
Published: December 2023

Abstract
In this paper, an efficient technique for stabilizing the bandwidth of low-bandwidth biomedical gm-c filters is proposed. Using this technique, the bandwidth of the filter becomes much less dependent on the circuit elements’ variations due to temperature or process, and thus the filter can be tuned more effectively and accurately. First, with circuit analysis, the reason for the dependency of the bandwidth on the circuit components is extracted. Then, an efficient circuit is proposed to reduce this dependency. The proposed 4th order gm-c filter has a symmetrical structure, and designed in TSMC 180 nm CMOS technology with bandwidth of 50 mHZ to 20 Hz, power consumption of 0.73 µW. Finally, using mathematical relationships and circuit simulations, the effectiveness of the proposed filter is proven.