bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term experience in treatment of acute promyelocytic leukemia in Mexican children in a tertiary care hospital
Authors: Murillo-Maldonado Marco Antonio , Galván Paulina González , Ortega Israel Parra , Morales Félix Gaytán , Villegas Luis Juárez , Acosta Elisa Dorantes 
Published: November 2023

IntroductionAcute promyelocytic leukemia (APL) is a rare myeloid leukemia subtype affecting adult and pediatric populations. APL constitutes 15-20% of all childhood AML in Latin America, compared to 7% in the non-Latino population. This leukemia has unique characteristics, such as its association with chromosomal translocations involving the retinoid acid receptor α (RARA) gene on chromosome 17. In addition, APL is also distinct from other AML subtypes due to its response to all-trans-retinoic acid (ATRA), which induces terminal granulocytic differentiation of blasts. Overall 5-year survival rates are generally reported to be greater than 80%.Materials and methodsA study was conducted from January 2008 to December 2022 applying the IC-APL 2006 treatment protocol. This case series reports the clinical results of 22 children with APL. In all cases, the diagnosis was made by bone marrow aspiration and evaluation of the t(15:17) or t(11:17) transcripts.ResultsWe identified 22 patients with APL, of whom 10 were female and 12 were male. Twelve patients debuted with coagulation abnormalities. The doses of anthracyclines varied according to the risk, with an average of 496.8 mgm2. The cardiological assessment was performed before and after chemotherapy, finding 2/22 patients with moderate sisto-diastolic dysfunction and one with mild pulmonary insufficiency at the end of treatment. There were 6/22 patients with complications related to ATRA treatment, the most frequent being pseudotumor cerebri. All complications were transitory and treated immediately without complications. In this series of cases, an overall survival of 90.6% and a relapse-free survival of 90.6% were recorded. The follow-up mean was 9.1 ± 3.8 years.ConclusionAPL is a highly curable disease when combined with ATRA and anthracyclines. In this series of cases, good long-term results were observed with the IC-APL 2006 protocol. However, in Latin America, the availability of drugs such as arsenic trioxide as the first line of treatment is an unresolved challenge.
ClickArr: a novel, high-throughput assay for evaluating β-arrestin isoform recruitment
Authors: French Alexander R. , Meqbil Yazan J. , van Rijn Richard M. 
Published: November 2023

Background: Modern methods for quantifying signaling bias at G protein–coupled receptors (GPCRs) rely on using a single β-arrestin isoform. However, it is increasingly appreciated that the two β-arrestin isoforms have unique roles, requiring the ability to assess β-arrestin isoform preference. Thus, methods are needed to efficiently screen the recruitment of both β-arrestin isoforms as they compete for a target GPCR in cells.Methods: We used molecular cloning to develop fusion proteins of the δ-opioid receptor (δOR), β-arrestin 1, and β-arrestin 2 to fragments of click beetle green and click beetle red luciferases. In this assay architecture, recruitment of either β-arrestin 1 or 2 to the δOR generates a spectrally distinct bioluminescent signal, allowing us to co-transfect all three constructs into cells prior to agonist challenge.Results: We demonstrate that our new assay, named “ClickArr,” is a live-cell assay that simultaneously reports the recruitment of both β-arrestin isoforms as they compete for interaction with the δOR. We further find that the partial δOR agonist TAN67 has a significant efficacy bias for β-arrestin 2 over β-arrestin 1 when recruitment is normalized to the reference agonist leu-enkephalin. We confirm that ClickArr reports this bias when run either as a high-throughput endpoint or high-throughput kinetic assay, and cross-validate this result using the PathHunter assay, an orthogonal commercial assay for reporting β-arrestin recruitment to the δOR.Conclusion: Our results suggest that agonist:GPCR complexes can have relative β-arrestin isoform bias, a novel signaling bias that may potentially open up a new dimension for drug development.
Comparison of bioelectrical body and visceral fat indices and anthropometric measures in relation to type 2 diabetes by sex among Chinese adults, a cross-sectional study
Authors: He Jiangshan , Zhang Binbin , Fan Yaqi , Wang Yuxue , Zhang Mianzhi , Li Chunjun , Zhang Li , Guo Pei , Zhang Minying 
Published: November 2023

ObjectivesWe aim to compare the efficacies of the bioelectrical indices (percentage of body fat, PBF; visceral fat area, VFA) with the conventional anthropometric measures (body mass index, BMI; waist-hip ratio, WHR) for predicting type 2 diabetes (T2D) risk by sex and to determine the sex-specific optimal adiposity indices to predict the T2D risk.DesignCross-sectional design.SettingTianjin First Central Hospital and Tianjin Union Medical Center, Tianjin, China.ParticipantsA total of 9,332 adults (41.35% men) undergoing physical examination.Primary and secondary outcome measuresT2D was defined using the WHO’s criteria: fasting blood glucose (FBG) ≥7.0 mmol/L and/or previous diagnosis of T2D. Height, weight, waist, hip, PBF, VFA, and fasting plasma glucose were measured.ResultsAll studied adiposity indices were associated with T2D among both males and females, and the observed associations differed by sex. The standardized aORs of BMI, WHR, PBF and VFA for T2D were 1.60 (95% CI 1.42–1.81), 1.43 (95% CI 1.25–1.64), 1.42 (95% CI 1.23–1.62) and 1.53 (95% CI 1.35–1.75) for females, and 1.47 (95% CI 1.31–1.66), 1.40 (95% CI 1.25–1.58), 1.54 (95% CI 1.36–1.74) and 1.47 (95% CI 1.31–1.65) for males, respectively. The AUCs of VFA, WHR and BMI were 0.743, 0.742 and 0.717 in women, respectively, whereas none of the indices had AUC larger than 0.70 in men. The AUCs were not significantly different between VFA and WHR, while both demonstrate larger AUCs than BMI and PBF in females (all p &lt; 0.05). The optimal cutoff values of VFA, WHR, and BMI for T2D in women were 103.55 cm2, 0.905, and 24.15 kg/m2, respectively.ConclusionAlthough BMI, WHR, and PBF and VFA as measured by bioelectrical impedance analysis (BIA) were all positively associated with T2D, their efficacy for predicting the risk of T2D differed by sex. VFA, WHR and BMI could be used as biomarkers to predict T2D risk in women, however none of the study indicators demonstrated favorable efficacy of predicting T2D risk in men.
Investigating the perceptual attribution of a virtual robotic limb synchronizing with hand and foot simultaneously
Authors: Sakurada Kuniharu , Kondo Ryota , Nakamura Fumihiko , Kitazaki Michiteru , Sugimoto Maki 
Published: November 2023

Introduction:Incorporating an additional limb that synchronizes with multiple body parts enables the user to achieve high task accuracy and smooth movement. In this case, the visual appearance of the wearable robotic limb contributes to the sense of embodiment. Additionally, the user’s motor function changes as a result of this embodiment. However, it remains unclear how users perceive the attribution of the wearable robotic limb within the context of multiple body parts (perceptual attribution), and the impact of visual similarity in this context remains unknown.Methods:This study investigated the perceptual attribution of a virtual robotic limb by examining proprioceptive drift and the bias of visual similarity under the conditions of single body part (synchronizing with hand or foot motion only) and multiple body parts (synchronizing with average motion of hand and foot). Participants in the conducted experiment engaged in a point-to-point task using a virtual robotic limb that synchronizes with their hand and foot motions simultaneously. Furthermore, the visual appearance of the end-effector was altered to explore the influence of visual similarity.Results:The experiment revealed that only the participants’ proprioception of their foot aligned with the virtual robotic limb, while the frequency of error correction during the point-to-point task did not change across conditions. Conversely, subjective illusions of embodiment occurred for both the hand and foot. In this case, the visual appearance of the robotic limbs contributed to the correlations between hand and foot proprioceptive drift and subjective embodiment illusion, respectively.Discussion:These results suggest that proprioception is specifically attributed to the foot through motion synchronization, whereas subjective perceptions are attributed to both the hand and foot.
Temporal and spatial evolution of agricultural carbon emissions and their impact on functional zoning: evidence from Hubei Province
Authors: Chen Qiao , Mao Yan , Cheng Jianquan 
Published: November 2023

IntroductionTo investigate the spatiotemporal evolution of agricultural carbon emissions and carbon absorption, analyse the spatiotemporal variations in the carbon balance, delineate carbon-offsetting regions, and formulate low-carbon development strategies tailored to various major functional zones, this study aims to promote coordinated regional ecological and environmental governance.MethodsThis study takes a perspective based on major functional zones, focuses on 17 cities in Hubei Province, studies the spatiotemporal variations in agricultural carbon budgets and carbon offsets in each city from the perspective of functional zoning and proposes a spatial optimization scheme for reducing carbon emissions.Results and discussionThe results show that both agricultural carbon emissions and carbon absorption in Hubei Province gradually increased, although the agricultural carbon budgets varied significantly among cities. Arable lands were the main agricultural carbon sinks in Hubei Province. Overall, carbon emissions exhibited declining core–periphery zonation, with Xiangyang, Jingzhou, and Huanggang serving as the centre (high emissions) and the cities of Shennongjia, Enshi, and Yichang serving as the periphery (low emissions). Carbon absorption displayed a U-shaped distribution, with high values in the east, south, and west and low values in the centre and north. The cities of Yichang, Jingmen, and Huanggang were the peak carbon sink areas. In recent years, the coordination between the agricultural carbon emissions and carbon budgets in Hubei Province has gradually improved, and agricultural carbon absorption and emissions have become increasingly balanced. Seven carbon-positive, five carbon-neutral, and five carbon-negative areas were identified in the province. Based on these findings, differentiated carbon emission reduction strategies were proposed to promote coordinated and low-carbon agriculture.
Yeast Lipids from Crude Glycerol Media and Utilization of Lipid Fermentation Wastewater as Maceration Water in Cultures of Edible and Medicinal Mushrooms
Authors: Diamantis Ilias , Papanikolaou Seraphim , Michou Savvoula , Anastasopoulos Vassilios , Diamantopoulou Panagiota 
Published: November 2023

Four wild “red” yeast strains (Rhodosporidium kratochvilovae FMCC Y70, R. toruloides NRRL Y-27013, R. toruloides NRRL Y-17902 and R. toruloides NRRL Y-6985) were cultured in shake flasks on industrial glycerol at an initial substrate (Gly0) concentration ≈ 50 g/L under nitrogen limitation. Strains NRRL Y-27013, NRRL Y-17902 and NRRL Y-6985 presented appreciable dry cell weight (DCW) and lipid synthesis (DCW up to 18–19 g/L containing lipids in quantities ≈ 47%, w/w). Strains NRRL Y-27013 and NRRL Y-6985 were further tested in higher Gly0 concentrations (≈90 g/L and ≈110 g/L) with the same initial nitrogen quantity as in the first (“screening”) experiment. Both strains, despite the high Gly0 concentrations and C/N ratios (up to 120 moles/moles) imposed, presented significant DCW production (up to c. 29.0–29.5 g/L). Yeast biomass contained significant lipid (42–43%, w/w) and endopolysaccharide (up to 42%, w/w) quantities. Both lipids and endopolysaccharide quantities (in % w/w) noticeably increased as a response to the imposed nitrogen limitation. Lipids containing mainly oleic and palmitic acids constituted ideal candidates for biodiesel synthesis. Thereafter, the wastewaters derived from the lipid production process (lipid fermentation wastewaters—LFWs) were used as maceration waters in cultivations of edible and medicinal fungi, where novel (non-conventional) substrates were used in the performed cultures. CW (coffee residue + wheat straw), CB (coffee residue + beech wood shavings), OW (olive crop + wheat straw), OB (olive crop + beech wood shavings), RW (rice husk + wheat straw) and RB (rice husk + beech wood shavings) were soaked/sprayed with LFWs or tap water and utilized in the cultivation of Pleurotus, Ganoderma and Lentinula mushrooms. The impact of LFWs on the mycelial growth rate (mm/d) and biomass production was evaluated. The results show that regardless of the wetting method, the highest growth rates (6.2–6.6 mm/d) were noticed on RW and RB for Pleurotus eryngii and Ganoderma resinaceum, on OW, OB and RW for Ganoderma applanatum and on RW, OW and OB for Lentinula edodes. Nevertheless, high biomass production was obtained on substrates soaked with LFWs for Pleurotus ostreatus (RW: 443 mg/g d.w.), L. edodes (RB: 238 mg/g d.w.) and Ganoderma lucidum (RW: 450 mg/g d.w.). Overall, this study demonstrates the possibility of the industrial conversion of low-value agro-waste to mycelial mass and eventually to important food products.
Analisa untuk Pengembangan E-Learning Universitas X pada Pasca Pandemi
Authors: Sutoyo Mochammad Arief Hermawan Sutoyo , Setiawan Roby 
Published: November 2023

Pasca pandemi penggunaan e-learning tidak wajib dilakukan pada Universitas X, dikarenakan dilakukannya kembali kuliah secara luring, sehingga penggunaan aplikasi yang telah dibangun tidak digunakan maksimal seperti aplikasi EDMODO yang digunakan sebelumnya sebagai sistem e-learning Universitas X. Perubahan kondisi ini tidak hanya terjadi pada Universitas X tetapi juga pada tempat lain. Perubahan kondisi ini menyebabkan perlunya penelitian terkait dampak dari penggunaan pasca pandemi terhadap e-learning yang digunakan saat ini oleh mahasiswa dan dosen Universitas X. Penelitian ini dilakukan secara kuantitatif menggunakan kuesioner dengan instrumen system usability scale dengan responden civitas akademika Universitas X. Berdasarkan perhitungan yang dilakukan didapatkan nilai SUS e-learning Universitas X yaitu 68,97. Nilai tersebut menyatakan bahwa sistem yang digunakan saat ini dikategorikan cukup baik. Namun nilai SUS sistem e-learning yang digunakan saat ini lebih rendah daripada nilai yang didapatkan pada penelitian sebelumnya menggunakan EDMODO sebagai aplikasi e-learning.
ПАТРІОТИЧНІ МОТИВИ В ЛІРИЦІ ВЛАДЛЕНА КОВТУНА
Authors: Немченко Іван 
Published: November 2023

У статті розглядаються ліричні твори патріотичної тематики з доробку сучасного українського поета В. Ковтуна. До аналізу залучено тексти, присвячені Україні та національним світочам, малій батьківщині письменника, природі рідного краю. Особливу увагу приділено патріотичним віршам, котрі стали відомими піснями. Простежено зв’язок творчості митця з українським фольклором.
Smart Roads: Roadside Perception, Vehicle-Road Cooperation and Business Model
Authors: Chen Rui , Gao Lu , Liu Yutian , Guan Yong Liang , Zhang Yan 
Published: November 2023

<p>Smart roads have become an essential component of intelligent transportation systems (ITS). The roadside perception technology, a critical aspect of smart roads, utilizes various sensors, roadside units (RSUs), and edge computing devices to gather real-time traffic data for vehicle-road cooperation. However, the full potential of smart roads in improving the safety and efficiency of autonomous vehicles only can be realized through the mass deployment of roadside perception and communication devices. On the one hand, roadside devices require significant investment but can only achieve monitoring function currently, resulting in no profitability for investors. On the other hand, drivers lack trust in the safety of autonomous driving technology, making it difficult to promote large-scale commercial applications. To deal with the dilemma of mass deployment, we propose a novel smart-road vehicle-guiding architecture for vehicle-road cooperative autonomous driving, based on which we then propose the corresponding business model and analyze its benefits from both operator and driver perspectives. The numerical simulations validate that our proposed smart road solution can enhance driving safety and traffic efficiency. Moreover, we utilize the cost-benefit analysis (CBA) model to assess the economic advantages of the proposed business model which indicates that the smart highway that can provide vehicle-guided-driving services for autonomous vehicles yields more profit than the regular highway.</p>
EXODONTIA DE DENTE SUPRANUMERÁRIO UNITÁRIO EM MANDIBULA: RELATO DE CASO
Authors: Rosa Tamily dos Santos , Oliveira Joyce Kristina , Rodrigues Andressa Lopes da Silva 
Published: November 2023

Introdução: Dentes supranumerários são descritos como uma deformidade dentária gerada pelo aumento da quantidade de germes dentários de permanente ou decíduos. Sua maior prevalência é na região frontal da maxila ou em região de mandíbula nos pré-molares, podendo ser únicos ou múltiplos, unilaterais ou bilaterais. Objetivo: Mostrar a importância do diagnóstico e remoção cirúrgica do elemento supranumerário, através de relato clínico, e, ressaltar a valia de solicitar exames complementares bem como exame clínico criterioso no início de tratamentos odontológicos. Relato de caso: Paciente, sexo feminino, 15 anos e 4 meses de idade, procurou a clínica odontológica da Faculdade FIMCA Unicentro, portando um exame de imagem tipo tomografia computadorizada e panorâmica em mãos. Observou-se a presença de um dente supranumerário entre os dentes 33 e 34 localizados em mandíbula. Discussão: deve ser considerada uma dentição supranumerária quando a quantidade de dentes é superior a vinte dentes decíduos ou trinta e dois dentes permanente. A indicação de tratamento de dentes supranumerários é a remoção cirúrgica, porém depende da idade do paciente e se há riscos de danos aos tecidos adjacentes e elementos dentários. Conclusão: O procedimento foi realizado com sucesso, sem intercorrências. A paciente não relatou dor ou edema durante o pós-operatório e após a cicatrização foi encaminhada novamente para iniciar o tratamento ortodôntico.
Introduction: Supernumerary teeth are described as a dental deformity created by the increase in the quantity of permanent or deciduous tooth germs. The most common occurrence is in the frontal region of the maxilla and in the region of the mandibles in the premolars and they can be singular or multiple, unilateral or bilateral. Objective: To show the importance of diagnosis and surgical removal of the supernumerary element through a clinical report. We also highlight the value of requesting complementary exams as well as making a thorough clinical examination at the beginning of the dental treatment. Case report: A female patient of 15 years and 4 months of age, sought out the dental clinic of the FIMCA Unicentro University carrying a computerized panoramic tomography in her hands. The presence of supernumerary teeth was detected between teeth number 33 and 34 located in the mandible. Discussion: The teething is considered supernumerary when there are more than twenty deciduous teeth or thirty-two permanent teeth. The recommended treatment for supernumerary teeth is surgical removal. However, it depends on the age of the patient if there is a risk of damage to the adjacent tissues and dental elements. Conclusion: The procedure was conducted successfully without any complications. The patient did not report pain or edema during the post-operatory phase and after healing she was referred to retake her orthodontic treatment.
Comparison of pharmacokinetic parameters calculation techniques in studies with animal-point design
Authors: Kosman V. M. , Karlina M. V. , Makarоva M. N. , Makarov V. G. 
Published: November 2023

In pharmacokinetics (PK) studies of medicinal products with small laboratory animals models, primarily rodents, the design of the animal-point experiment is often used, involves the selection of biological material after euthanasia of the animal. The question of experimental data processing and the PK parameters calculation method in a situation where all concentration values are obtained from diﬀerent individuals is relevant.Purpose of the study. Comparison of pharmacokinetic parameters calculation methods in studies with the animal-point design.Materials and methods. For a number of previously conducted studies with male outbred rats test systems, a retrospective data analysis was performed and PK parameters were calculated in three diﬀerent ways: from the average concentration values at each time point (method 1): from data obtained for animals with the same sequence numbers in subgroups corresponding to time points (method 2); using resempling based on modeling of individual PK proﬁles (method 3). Pharmacokinetic parameters (maximum concentration — Cmax, time to reach maximum concentration — Tmax, area under the curve "concentration-time" — AUC0-t, average time to stay in the body — MRT, half-life — T1/2) were calculated by non-compartment method of statistical moments using the validated PKSolver application for Microsoft Oﬃce Excel.Results. The comparison of the obtained results did not reveal any patterns and preferences for the use of a particular method of calculating PK parameters depending on the studied drugs, route and administration way. For all evaluated PK parameters (Cmax, Tmax, AUC0-t, MRT, T1/2), similar values and/or intervals were obtained, which indicated the correctness of all considered calculation methods.Conclusion. Based on advantages and disadvantages of the calculation methods comparison it is shown that it is optimal to use method 2, which is a special case of reception (method 3) with a minimum number of replications. It is important to emphasis the method of PK parameters calculation when describing the methodology of studies to improve their quality.
Neuropeptide cyclo-L-prolylglycine counteracts scopolamine-induced long-term memory impairment in rats in the novel object recognition test
Authors: Vorontsova O. N. , Alyaeva A. G. , Koliasnikova K. N. , Valdman E. A. , Povarnina P. Yu. 
Published: November 2023

Background. Cyclo-L-prolylglycine (CPG) was designed and synthesized at the V.V. Zakusov as a topological analogue of the classical nootrop piracetam and was further identiﬁed as an endogenous compound. Previously, the nootropic eﬀect of CPG was revealed in a model of retrograde amnesia in rats induced by electroconvulsive shock in the passive avoidance test (PAT).Objective. The aim of the present study was to investigate the nootropic eﬀect of CPG under more physiological conditions in the absence of strong stressors.Methods. Amnesia in rats was modeled by intraperitoneal (ip) administration of scopolamine at a dose of 2 mg/kg. CPG was administered ip at doses of 0.1 and 1.0 mg/kg 15 minutes after scopolamine. Short- and long-term memory were recorded in the novel object recognition test.Results. It was found that scopolamine disrupted only the long-term memory of rats. CPG at a dose of 0.1 mg/ kg almost completely counteracted this impairment. CPG by itself had no eﬀect on memory at both doses studied.Conclusion. Thus, CPG exhibits nootropic activity not only in the aversive conditions of the PAT and electroconvulsive shock-induced amnesia, but also in the neutral situation in the novel object recognition test, when the amnesia was caused by the administration of scopolamine.