bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mother–child similarity during observed interactions: Links with mother and child effortful control
Authors: Hong Yelim , Bell Martha Ann , Deater‐Deckard Kirby 
Published: February 2024

AbstractObjectiveWe examined whether mothers' and child's regulatory capacity, measured by effortful control (EC) and its facets, is associated with variance in mother–child within‐dyad similarity in multiple aspects of positive emotions and behaviors during their interaction.BackgroundBoth parents' and children's self‐regulation capacities may help them to modulate their behaviors and emotions to be mutually responsive to and harmonious with their interaction partner.MethodsParticipants were children of 3‐ to‐7‐year‐olds and their mothers (n = 147). Mothers completed a set of questionnaires regarding themselves, their child, and demographics, and mother–child interactions were observed during three cooperative tasks. We estimated mother–child within‐dyad similarity during their brief interaction using the Q‐correlation method.ResultsMothers' general EC was statistically associated with greater mother–child similarity, but children's EC was not. When testing the subscales of EC, greater mother–child similarity was associated with mother's higher attentional control, child's better attention focusing, child's lower levels of low intensity pleasure, and older child age.ConclusionMother's higher attentional control and child's better attention focusing are associated with greater mother and child within‐dyad similarity during their interactions.ImplicationsThis study highlights the importance of considering child and mother self‐regulation for intervention efforts to better understand how dyads match and co‐modulate their behaviors and emotions during their interactions.
“Privileged adult”: How relationship with parents influences adult's status consumption
Authors: Huang Yunzhi , Zhou Kun , Yang Ying , Xu Man 
Published: February 2024

ABSTRACTObjectiveTo explore why individuals engage in status consumption despite scarce monetary resources in the Chinese context. This exploration employs a family influence perspective focusing on whether the parental relationship is associated with the status consumption tendency of individuals.MethodThree surveys were conducted, and the responses of 637 Chinese participants were used to test the hypothesis systematically.ResultsParticipants who had a close relationship with their parents were more likely to engage in status consumption, had a more positive attitude toward luxury brands, and spent more money on products that signal status. This effect is driven by participants' perceived social support from close parental relationship. This positive effect of parental relationship on the status consumption holds when their monetary resources are scarce but disappears when their monetary resources are abundant.ConclusionThe findings suggest that a close relationship with parents increases status consumption. When engaging in status consumption, social support received from parents and monetary resources are interchangeable.ImplicationsIt is important to cultivate an appropriate consumption concept in interactions with the next generation.
Dialogue journals and traditional writing assignments: A comparison of writing in L2 German classrooms
Authors: Henry Nick , Mandel Hannes , VanderHeijden Vincent 
Published: February 2024

AbstractDialogue journals (DJs) are a type of extensive writing assignment, in which students write for the purpose of communication with their instructor. Previous research has shown that DJs can help students develop writing skills; however, few studies have analyzed how DJs compare with traditional prompt‐based assignments (TAs). The present study, therefore, presents a quasi‐experimental comparison of texts from first‐ and second‐year German classrooms that used either DJs or TAs. Analysis focused on five metrics: (1) word count, (2) sentence count, (3) words per sentence, (4) text‐type ratio (TTR), and (5) measure of textual lexical diversity (MTLD). Results for word and sentence count indicated that TA texts were longer. However, DJs resulted in more complex writing, as measured by sentence length, MTLD, and TTR. Taken together, results suggest that journals can promote writing that is both communicatively oriented and linguistically complex. The article concludes by offering suggestions for practical implementation of DJs.
Cathepsin L prevents the accumulation of alpha‐synuclein fibrils in the cell
Authors: Matsuki Ayumi , Watanabe Yoshihisa , Hashimoto Sho , Hoshino Atsushi , Matoba Satoaki 
Published: February 2024

AbstractThe deposition of α‐synuclein (α‐Syn) fibrils in neuronal cells has been implicated as a causative factor in Parkinson's disease (PD) and dementia with Lewy Bodies (DLB). α‐Syn can be degraded by autophagy, proteasome, and chaperone‐mediated autophagy, and previous studies have suggested the potency of certain cathepsins, lysosomal proteases, for α‐Syn degradation. However, no studies have comprehensively evaluated all cathepsins. Here, we evaluated the efficacy of all 15 cathepsins using a cell model of α‐Syn fibril propagation and found that overexpression of cathepsin L (CTSL) was the most effective in preventing the accumulation of α‐Syn aggregates. CTSL‐mediated degradation of α‐Syn aggregates was dependent on the autophagy machinery, and CTSL itself promoted autophagy flux. Interestingly, CTSL was effective in autophagic degradation of wild‐type (WT) α‐Syn, but not in the case of A53T and E46K missense mutations, which are causative for familial PD. These results suggest that CTSL is a potential therapeutic strategy for sporadic PD pathology in WT α‐Syn.
Isoliquiritigenin inhibits NLRP3 inflammasome activation with CAPS mutations by suppressing caspase‐1 activation and mutated NLRP3 aggregation
Authors: Usui‐Kawanishi Fumitake , Kani Koudai , Karasawa Tadayoshi , Honda Hiroe , Takayama Nobuyuki , Takahashi Masafumi , Takatsu Kiyoshi , Nagai Yoshinori 
Published: February 2024

AbstractThe nucleotide‐binding oligomerization domain leucine‐rich repeat and pyrin domain containing 3 (NLRP3) inflammasome contributes to the development of inflammatory diseases. Cryopyrin‐associated periodic syndrome (CAPS) is an autoinflammatory disease caused by NLRP3 gene mutations that results in excessive IL‐1β production. We previously identified isoliquiritigenin (ILG), a component of Glycyrrhiza uralensis extracts, as a potent inhibitor of the NLRP3 inflammasome. Here, we aimed to investigate whether ILG inhibits the activation of NLRP3 inflammasome caused by NLRP3 gene mutations. We demonstrated that ILG significantly inhibited NLRP3 inflammasome‐mediated lactate dehydrogenase (LDH) release and IL‐1β production in two CAPS model THP‐1 cell lines, NLRP3‐D303N and NLRP3‐L353P, in a dose‐dependent manner. Interestingly, the NLRP3 inhibitor MCC950 inhibited LDH release and IL‐1β production in NLRP3‐D303N cells, but not in NLRP3‐L353P cells. Western blotting and caspase‐1 activity assays showed that ILG, as well as caspase inhibitors, including Z‐VAD and YVAD, suppressed caspase‐1 activation. Notably, ILG prevented cryo‐sensitive foci formation of NLRP3 without affecting the levels of intracellular Ca2+. We concluded that ILG effectively prevents the constitutive activation of the inflammasome associated with NLRP3 gene mutations by inhibiting the aggregation of cryo‐sensitive mutated NLRP3.
Is hemodiafiltration superior to high‐flow hemodialysis in reducing all‐cause and cardiovascular mortality in kidney failure patients? A meta‐analysis of randomized controlled trials
Authors: Bignardi Paulo Roberto , Delfino Vinicius Daher Alvares 
Published: February 2024

AbstractIntroductionHemodiafiltration (HDF) and high‐flux hemodialysis (hf‐HD) are different methods of kidney replacement therapy (KRT) used for the treatment of kidney failure patients. A debate has raged over the last decade about the survival benefit of patients with the use of HDF compared with hf‐HD, but with divergent results from randomized controlled trials. Therefore, this study aimed to perform a meta‐analysis to compare HDF and hf‐HD regarding all‐cause and cardiovascular mortality.MethodsPubMed and Cochrane databases were searched until July 19, 2023, for randomized clinical trials comparing HDF and hf‐HD in patients on maintenance dialysis. A meta‐analysis was performed using Stata 16.1, applying fixed or random effect models according to the heterogeneity between studies.FindingsOf the 496 studies found, five met the inclusion criteria. Compared with the hf‐HD group, the risk ratio (RR) for all‐cause mortality with HDF use was 0.76 (95% CI: 0.67–0.88, I2 = 0%). HDF was associated with lower cardiovascular mortality, although the sensitivity analysis showed that the result differed between scenarios. Subgroup analysis showed lower all‐cause mortality among patients without diabetes in the HDF group compared with hf‐HD (RR 0.66, 95% CI: 0.51–0.81, I2 = 0%), but not in diabetic patients (RR = 0.89, 95% CI: 0.65–1.12, I2 = 0.0%). A subgroup analysis considering convection volumes was not performed, but the studies with the highest weight in the meta‐analysis described convection volume as more than 20 L/session.DiscussionMore clinical studies considering critical risk factors, such as advanced age and preexisting cardiovascular disease, are needed to confirm the supremacy of HDF over hf‐HD on the survival of patients treated by these two forms of kidney replacement therapy.
The ideology of crisis in higher education
Authors: Macfarlane Bruce 
Published: February 2024

AbstractHigher education seems to be in a perpetual state of ‘crisis’. The many hundreds of books and papers containing this specific, or other relevantly similar expressions, convey a sense of fear and angst. Yet, what are these various crises about, and which values and beliefs are seen as threatened or ‘under attack’? This paper will provide an analysis of the ‘crisis’ literature and identify four major crisis themes – massification, marketisation, restitution and geopolitics, linked to their ideological basis and influences—including meritocracy, liberalism, restorative justice, and justice globalism. The second part of the paper analyses the massification crisis in Britain between the 1940s and the 1970s as a case example identifying how the principles of a meritocratic society played an influential role in the debate. It will be argued that the notion of ‘universities in crisis’ needs to be understood critically in terms of ideology and historically via the shifting and reshaping of such beliefs over time.
Are codesigned programmes more difficult to implement? A qualitative study of staff perceptions on the implementation of a new youth mental health programme
Authors: Kehoe Michelle , Whitehead Rick , de Boer Kathleen , Meyer Denny , Hopkins Liza , Nedeljkovic Maja 
Published: February 2024

AbstractBackgroundCodesigned interventions are becoming more common in health services and, in particular, in the design and development of mental health programmes and interventions. However, previous research has established that the transition from codesign to implementation can experience several challenges and that this transition process has received little research attention.ObjectiveThe aim of this study was to explore the experience of staff members charged with the implementation of a codesigned intervention for young people and adolescents at risk of suicide.Setting and ParticipantsFive staff members involved in the implementation of the new codesigned programme took part in semi‐structured interviews.MethodThe study involved qualitative evaluation of staff experiences during the implementation of a new child and youth suicide intervention. Interviews were analysed using reflexive thematic analysis.ResultsThe analysis identified four themes of ‘disconnect’, ‘operational challenges, ‘service user’ and ‘being authentic’. ‘Disconnect’ captures the difficulties of implementing a codesigned programme which leads to ‘operational challenges’ in meeting broader expectations while ensuring the feasibility of the programme. The third theme, ‘service user’, captures the realisation that the young people accessing the new service were different to those involved in the codesign process. The final theme, ‘being authentic’, highlights how staff needed to be responsive and flexible while remaining true to the principles proposed in the codesign.DiscussionThis study yielded some valuable insights into the challenges around the implementation of a codesigned intervention, an under‐researched area. The findings suggest that adaption of the design may be necessary, if it is not informed by implementation constraints, making it necessary for the implementation team to be well‐briefed on the initial design and given plenty of time to make the necessary adjustments in a coproduction process. Limitations for the generalisation of the results include a small sample of staff and particular challenges that may be unique to this study.ConclusionThe present study highlights that for health services undertaking codesign approaches, appropriate time and resources need to be considered for the implementation phase of an initiative, to ensure that there is effective translation from design to implementation and that new codesigned services can be effective within operational constraints.Patient and Public ContributionThe authors would like to thank and acknowledge the young people with a lived‐experience and their carers who participated in the codesign process and research evaluation component of this study. We also wish to thank the clinical staff, peer workers and family peer workers who participated in the evaluation.
Tetraploid wheat (Triticum turgidum) lines from the United States as a source of Hessian fly (Mayetiola destructor) resistance
Authors: Subramanyam Subhashree 
Published: February 2024

AbstractDeploying resistant wheat cultivars is the most economical and environment‐friendly strategy to manage the devastating effects of the dipteran pest, Hessian fly (Hf; Mayetiola destructor). Currently, 37 Hf resistance genes have been identified to combat the 18 Hf genotypes documented so far. However, the Hf populations adapt rapidly to overcome the newly deployed resistance genes within a few years of release resulting in development of virulent Hf biotypes and breakdown of plant resistance. Identification of new and novel sources of resistance offers breeders additional resources that can be included in the breeding programmes to develop elite Hf‐resistant cultivars. In the current study, we screened 374 wheat (tetraploid and hexaploid) accessions originating from different regions of the United States and identified three tetraploid (Triticum turgidum) pasta wheat lines, one originating from North Dakota (PI 639869) and two from Minnesota (PI 352398 and CItr 15710) exhibiting ≥95% resistance to Hf biotype L at 20°C. Further, the wheat cultivar PI 352398 showed 100% resistance to six additional Hf genotypes including biotypes B, C, D, O, GP and vH13. The lines PI 639869 and CItr 15710 also showed >70% resistance with most biotypes, except against biotype GP with the former and biotype B with the latter. Interestingly, a few plants from these two cultivars exhibited putative tolerance to these biotypes where the plants showed normal growth but harboured white, live larvae and showed cell permeability that was intermediate in levels between Hf‐infested resistant and susceptible wheat. Additionally, since the increase in environmental temperatures to 25–30°C also negatively impacts Hf resistance, the three T. turgidum (PI 639869, PI 352398 and CItr 15710) cultivars were evaluated for Hf resistance at 30°C. None of the wheat cultivars were resistant to Hf biotype L at 30°C indicating a temperature‐dependent breakdown of resistance and are therefore not suitable for geographical regions with higher environmental temperatures. Taken together, these three wheat lines represent additional sources of Hf resistance that can be leveraged by breeders for developing durable elite lines.
Identification of Isthmin‐1 in common carp (Cyprinus carpio L.) and the effects on glucose metabolism in vivo and in vitro
Authors: Qu Leya , Yang Liping , Wang Yiran , Zhi Shaoyang , Zhao Mengjuan , Xiong Jinrui , Yan Xiao , Qin Chaobin , Nie Guoxing 
Published: February 2024

AbstractIsthmin‐1 (Ism1) plays roles in glucose uptake in mammals as an adipokine. To investigate its role in the glucose metabolism of common carp (Cyprinus carpio. L), the Ism1 sequence was cloned, and its expression and distribution in tissues were detected. In addition, we prepared and purified the recombinant Ism1 protein using the E. coli expression system and assessed changes in the expression of key genes related to glucose metabolism through both in vivo injection experiments and primary hepatocyte experiments in vitro. The results revealed that the open reading frame of Ism1 was 1377 bp long, encoding 458 amino acids. Similarity analysis indicated that Ism1 exhibited a close evolutionary relationship with goldfish (Carassius auratus), sharing 98.35% amino acid similarity. Ism1 was expressed in all tissues of common carp, with the highest level observed in the heart, followed by the gill, head kidney, and hepatopancreas. Distinct patterns of Ism1 expression were identified during the oral glucose tolerance test and long‐term high‐carbohydrate and high‐fat diet feeding experiments. In vivo studies demonstrated that the serum glucose concentration was reduced on treatment with Ism1, accompanied by a significant upregulation of mRNA levels for gk, hk, and pfk genes in hepatopancreas; conversely pepck and g6pase mRNA levels were significantly downregulated in the hepatopancreas under these conditions as well. Furthermore, our primary hepatocyte experiment confirmed that Ism1 could inhibit pepck and g6pase mRNA expression, while promoting gk, hk, and pfk mRNA expression levels. In conclusion, Ism1, in common carp, could participate in the glucose metabolism, which provides essential information for future studies on the function of Ism1.
Linkage between spatiotemporal distribution of environmental DNA and phenological activity in an amphidromous fish, ayu Plecoglossus altivelis altivelis, in a river located in its northernmost distributional area
Authors: Yonago Takuma , Kawakami Tatsuya , Kasai Akihide 
Published: February 2024

AbstractEnvironmental DNA (eDNA) is a promising tool for the continuous monitoring of fish ecology and diversity. However, its potential for describing the phenological activity of fish has rarely been examined. This study aimed to elucidate a linkage between the spatiotemporal distribution of eDNA and the phenology of an amphidromous fish, ayu Plecoglossus altivelis altivelis, in a river in Hokkaido, Japan, which is its northernmost distributional area. A significant positive correlation between eDNA concentration and catch per unit effort of P. a. altivelis in the river confirmed the use of eDNA as a surrogate for the abundance of P. a. altivelis. eDNA of P. a. altivelis was first detected in late April on a sandy beach adjacent to the river mouth. Subsequent to its first detection at the lowest site in the river in early May, eDNA spread throughout the river, indicating the upstream migration of P. a. altivelis. Spawning activity was also represented by a rapid increase in eDNA concentration and its surge at night in the lowest reaches of the river during September and October. These results suggest that upstream migration and spawning primarily commenced when the water temperature reached 10°C and decreased below 20°C, respectively. This observation is consistent with the behavioral responses observed in P. a. altivelis populations from other regions of Japan. Consequently, this study demonstrated that eDNA distribution was closely linked to the phenological activity of P. a. altivelis and that eDNA is a powerful tool for studying the phenology of migratory fishes.
Comparative phenotype and transcriptome analysis revealed the role of ferric uptake regulator (Fur) in the virulence of Vibrio harveyi isolated from diseased American eel (Anguilla rostrata)
Authors: Wan Qijuan , Zhai Shaowei , Chen Minxia , Xu Ming , Guo Songlin 
Published: February 2024

AbstractVibrio harveyi is commonly found in salt and brackish water and is recognized as a serious bacterial pathogen in aquaculture worldwide. In this study, we cloned the ferric uptake regulator (fur) gene from V. harveyi wild‐type strain HA_1, which was isolated from diseased American eels (Anguilla rostrata) and has a length of 450 bp, encoding 149 amino acids. Then, a mutant strain, HA_1‐Δfur, was constructed through homologous recombination of a suicide plasmid (pCVD442). The HA_1‐Δfur mutant exhibited weaker biofilm formation and swarming motility, and 18‐fold decrease (5.5%) in virulence to the American eels; compared to the wild‐type strain, the mutant strain showed time and diameter differences in growth and haemolysis, respectively. Additionally, the adhesion ability of the mutant strain was significantly decreased. Moreover, there were 15 different biochemical indicators observed between the two strains. Transcriptome analysis revealed that 875 genes were differentially expressed in the Δfur mutant, with 385 up‐regulated and 490 down‐regulated DEGs. GO and KEGG enrichment analysis revealed that, compared to the wild‐type strain, the type II and type VI secretion systems (T2SS and T6SS), amino acid synthesis and transport and energy metabolism pathways were significantly down‐regulated, but the ABC transporters and biosynthesis of siderophore group non‐ribosomal peptides pathways were up‐regulated in the Δfur strain. The qRT‐PCR results further confirmed that DEGs responsible for amino acid transport and energy metabolism were positively regulated, but DEGs involved in iron acquisition were negatively regulated in the Δfur strain. These findings suggest that the virulence of the Δfur strain was significantly decreased, which is closely related to phenotype changing and gene transcript regulation.
Integrating direct messaging with flood alerts and warnings: Insights into effectiveness from a registered public user population
Authors: Geddes Alistair , Black Andrew R. , Cranston Michael 
Published: February 2024

AbstractDirect messaging involving simultaneous mass transmission of brief text or voice messages to large numbers of recipients has become a frontline method in flood hazard communications. Messages are intended to serve as cues, drawing recipients' attention to changing conditions, yet the actual effectiveness of direct messaging among recipient groups remains under‐examined. This article considers direct messaging within the Floodline public flood warning service in Scotland, implemented by the Scottish Environment Protection Agency (SEPA). Within Floodline, messaging is integrated with alerting and warning information, termed straightforwardly ‘Flood Alerts’ and ‘Flood Warnings’. Collaborating with SEPA, we conducted an online questionnaire survey of registered Floodline direct messaging recipients. In this article, our analysis focusses specifically on responses to three open‐ended questions included in this survey, with an iterative qualitative coding approach employed to interpret themes of meaning from the question responses. This analysis gives a clear indication that recipients value Floodline and direct messaging. However, there are also questions raised over the utility of Flood Alerts and related messaging, which we elaborate in the findings and discussion, along with the scope for adding content, linking to other information, and developing closer relationships. Changes being developed by SEPA align with several of these findings.