bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attitudes towards hearing difficulties, health-seeking behaviour, and hearing aid use among older adults in Thailand
Authors: Pornprasit Panicha , Utoomprurkporn Nattawan , Areekit Poonsub , Manum Pornnapat , Thanutammakul Sutatta , Parmar Bhavisha , Adamson Joy , Bamiou Doris 
Published: January 2024

IntroductionThis qualitative study explores older adults' attitudes towards hearing difficulties, health-seeking behaviour and hearing aid use in Samutprakarn, Thailand.MethodThree focus groups (n=25), with adults aged 60-88 years, were conducted within a residential complex for older adults. Qualitative data analysis, employing a phenomenological approach was completed.ResultsFour themes are presented: (1) Perception of hearing difficulties, (2) Experience of receiving care, (3) Attitudes towards wearing hearing aids, and (4) Raising awareness of hearing difficulties in older adults. Findings indicate that Thai older adults may not be aware or accepting of their hearing difficulties, due to the stigma associated hearing loss, older age, and disability. The consequential impact of these unacknowledged issues manifest in communication challenges and the adoption of avoidance behaviours across diverse situational contexts.ConclusionThis study enhances our understanding of how Thai older adults perceive hearing problems and ear care. Findings underscore the potential pivotal role of familial and social networks in mitigating barriers associated with hearing difficulties among older adults in Thailand. These insights can raise awareness and inform effective management for older adults and their families. Additionally, the findings could guide healthcare providers, researchers, and policy makers, fostering improved care for this demographic.
Lipid accumulation product is an effective predictor of metabolic syndrome in non-obese women with polycystic ovary syndrome
Authors: Han Wenju , Zhang Meiwei , Wang Haiyan , Yang Yitian , Wang Lei 
Published: January 2024

ObjectiveTo explore the correlation of lipid accumulation product (LAP) with metabolic syndrome (MS) and to assess the predictive value of LAP for MS risk in polycystic ovary syndrome (PCOS) with different body mass index (BMI).MethodsA total of 242 PCOS patients and 150 controls were recruited and divided into normal-weight, overweight, and obese groups, then further divided into MS and without MS subgroups. Clinical and anthropometric variables and laboratory results were recorded. LAP was calculated from waist circumference (WC) and triglyceride using sex-specific formulae. Logistic regression analysis and receiver operating characteristic (ROC) curve were applied to determine and analyze the predictive value of LAP for MS.ResultsThe prevalence of MS among PCOS patients was 45.04%, which was significantly higher than that of the controls (10%). Stratified by BMI, the incidence of MS in the normal-weight, overweight, and obese PCOS groups were 15.58%, 41.43%, and 71.58%, respectively. Logistic regression analysis indicated that LAP was an independent risk factor for MS in both normal-weight and overweight groups; however, the results were not significant in the obese group. ROC curve analysis showed that LAP had an outstanding discrimination index for MS in normal-weight (AUC=0.960, cut-off value=42.5) and overweight (AUC=0.937, cut-off value=47.93) PCOS patients, with a sensitivity of 0.917/0.931 (normal-weight/overweight) and a specificity of 0.969/0.854 (normal-weight/overweight), respectively.ConclusionNormal-weight and overweight PCOS patients also have a fairly high incidence of MS and should receive as much attention as obese patients. Compared to applying multiple clinical indicators, LAP is more convenient and facilitates acquiring early and accurate diagnoses of MS among non-obese PCOS patients using fewer MS markers.
Gut microbiota and metabolites exhibit different profiles after very-low-caloric restriction in patients with type 2 diabetes
Authors: Gong Tong , Di Hongjie , Hu Yongxin , Xu Shuhang , Chen Jie , Chen Guofang , Wei Xiao , Liu Chao 
Published: January 2024

Background and aimsTo investigate the effect of short-term very-low-calorie restriction (VLCR) on metabolism in patients with type 2 diabetes (T2D), and elucidate the molecular mechanism through analyses on gut microbiota and small-molecule metabolites.MethodsFourteen T2D patients were hospitalized to receive VLCR (300-600 kcal/d) for 9 days. BMI, BP, and HR were taken before and after VLCR. Levels of blood lipids, fasting insulin, FBG, and 2h PBG were assessed. The microbial diversity in feces was detected by 16S rDNA high-throughput sequencing technology, and small-molecule metabolites in plasma and feces by untargeted metabolomics technology.ResultsAfter VLCR, BW, BMI, WC, BP, and levels of FBG and 2h PBG, insulin, HOMA-IR, and triglyceride decreased significantly in T2D patients (P&lt;0.05). There was no significant change in the α-diversity of fecal microbiota, but the abundance of Bacteroidetes increased significantly, and the Firmicutes/Bacteroidetes ratio decreased significantly from 11.79 to 4.20. Parabacteroides distasonis showed an abundance having increased most prominently after VLCR treatment. Plasma level of amino acid metabolite L-arginine increased significantly. Plasma levels of three lipid metabolites, PC (14:0/20:4 [8Z, 11Z, 14Z, 17Z]), LysoPC (16:1 [9Z]) and LysoPC (18:1 [11Z]), were significantly reduced. Fecal levels of lipid metabolite LysoPC (18:1 [11Z]) and bile acid metabolite glycholic acid were significantly decreased.ConclusionIn T2DM patients, VLCR can considerably reduce body weight and improve glucose and lipid metabolism without causing severe side effects. LysoPC (18:1 [11Z]) and Parabacteroides distasonis showed the most obvious difference after VLCR, which could be the indicators for VLCR in T2D.
Application and discoveries of metabolomics and proteomics in the study of female infertility
Authors: Shi Junhua , Wu Xingjie , Qi Haiou , Xu Xin , Hong Shihao 
Published: January 2024

IntroductionFemale infertility is defined as the absence of clinical pregnancy after 12 months of regular unprotected sexual intercourse.MethodsThis study employed metabolomics and proteomics approaches to investigate the relationship between metabolites and proteins and female infertility. The study used metabolomics and proteomics data from the UK Biobank to identify metabolites and proteins linked to infertility.ResultsThe results showed that GRAM domain-containing protein 1C and metabolites fibrinogen cleavage peptides ADpSGEGDFXAEGGGVR and 3-Hydroxybutyrate had a positive correlation with infertility, whereas proteins such as Interleukin-3 receptor subunit alpha, Thrombospondin type-1 domain-containing protein 1, Intestinal-type alkaline phosphatase, and platelet and endothelial cell adhesion molecule 1 exhibited a negative correlation. These findings provide new clues and targets for infertility diagnosis and treatment. However, further research is required to validate these results and gain a deeper understanding of the specific roles of these metabolites and proteins in infertility pathogenesis.DiscussionIn conclusion, metabolomics and proteomics techniques have significant application value in the study of infertility, allowing for a better understanding of the biological mechanisms underlying infertility and providing new insights and strategies for its diagnosis and treatment. These research findings provide a crucial biological mechanistic basis for early infertility screening, prevention, and treatment.
Research progress on animal habitat constructions from the perspective of urban biodiversity improvement
Authors: Liu Zhiruo , Yin Hao , Wang Yang , Cheng Qiushuang , Wang Zihan 
Published: January 2024

The accelerated urbanization process has caused problems such as habitat loss, isolation, and habitat quality decline, resulting in a sharp reduction in the richness and abundance of urban species. Constructing suitable habitat environmental conditions is the most direct and effective way to protect animals. In urban environment, habitat construction can be achieved by integrating species protection and landscape planning, which is also an important manifestation of biodiversity conservation at the ecosystem level. Understanding how to incorporate animal habitats into city plan and design is critical and urban planners would benefit from a review that holistically describes the steps and methods of animal habitat constructions. We conducted a review to highlight the animal habitats space resources and network structures. We synthesized the findings of research studies in the last 20 years to illuminate the investigation, assessment, planning and management of animal habitats. As habitat degradation and fragmentation in anthropogenic environments, our findings suggest city planners should consider ecological background investigation, habitat suitability assessment, habitat planning strategies and animal habitat management as four key steps of mitigation to alleviate these impacts. This study will provide a useful reference to improve animal survival quality and communication. Through this study, the consolidated research can aid in sustainable development and innovation to promote the ecological function of urban green space and the harmonious coexistence of humans and animals.
Estimation of hourly one square kilometer fine particulate matter concentration over Thailand using aerosol optical depth
Authors: Punpukdee Pongsakon , Winijkul Ekbordin , Kyaw Pyae Phyo , Virdis Salvatore G. P. , Xue Wenchao , Nguyen Thi Phuoc Lai 
Published: January 2024

In the recent years, concentration of fine particulate matter that are 2.5 microns or less in diameter (PM2.5) in Thailand has consistently exceeded the national ambient air quality standard. Currently, the measurement of PM2.5 concentration relies on air quality monitoring stations operated by the Pollution Control Department of Thailand (PCD). However, these stations are insufficient, particularly in rural areas, where agricultural open burning are major sources of pollution after harvesting period. This study aims to enhance the monitoring of PM2.5 concentration by leveraging cost-effective technologies. We propose the integration of satellite data, specifically Aerosol Optical Depth (AOD) from Multi-Angle Atmospheric Correction (MAIAC) product and Himawari-8 satellites, with the Weather Research and Forecasting Model (WRF) data, to provide supplementary data to the ground-based monitoring. Hourly 5 × 5 km2 AOD data from Himawari-8 were downscaled to a high-resolution of 1 × 1 km2, leveraging the AOD distribution pattern of the concurrent MAIAC product using eXtreme Gradient Boosting (XGBoost) model. Notably, during Thailand’s rainy season (May to August), the study observed a relative reduction in the training model’s R-square value. This phenomenon is attributed to temporal discrepancies between Himawari-8 and the MAIAC products during this period. The predictive models of PM2.5 concentrations with the identification of pertinent variables through Pearson’s correlation analysis and recursive feature elimination, driven by the robust XGBoost model. Subsequently, the downscaled AOD, wind speed, temperature, and pressure were identified as predictors for the estimation of hourly PM2.5 concentration. This comprehensive approach enabled the projection of PM2.5 levels across Thailand, encompassing over 600,000 grids at 1 × 1 km2 resolution. The developed models, thus, offer a valuable tool for robust and high-resolution PM2.5 concentration estimation, presenting significant implications for air quality monitoring and management in Thailand.
Exploring KRAS-mutant pancreatic ductal adenocarcinoma: a model validation study
Authors: Yang Fan , He Yanjie , Ge Nan , Guo Jintao , Yang Fei , Sun Siyu 
Published: January 2024

IntroductionPancreatic ductal adenocarcinoma (PDAC) has the highest mortality rate among all solid tumors. Tumorigenesis is promoted by the oncogene KRAS, and KRAS mutations are prevalent in patients with PDAC. Therefore, a comprehensive understanding of the interactions between KRAS mutations and PDAC may expediate the development of therapeutic strategies for reversing the progression of malignant tumors. Our study aims at establishing and validating a prediction model of KRAS mutations in patients with PDAC based on survival analysis and mRNA expression.MethodsA total of 184 and 412 patients with PDAC from The Cancer Genome Atlas (TCGA) database and the International Cancer Genome Consortium (ICGC), respectively, were included in the study.ResultsAfter tumor mutation profile and copy number variation (CNV) analyses, we established and validated a prediction model of KRAS mutations, based on survival analysis and mRNA expression, that contained seven genes: CSTF2, FAF2, KIF20B, AKR1A1, APOM, KRT6C, and CD70. We confirmed that the model has a good predictive ability for the prognosis of overall survival (OS) in patients with KRAS-mutated PDAC. Then, we analyzed differential biological pathways, especially the ferroptosis pathway, through principal component analysis, pathway enrichment analysis, Gene Ontology (GO) enrichment analysis, and gene set enrichment analysis (GSEA), with which patients were classified into low- or high-risk groups. Pathway enrichment results revealed enrichment in the cytokine-cytokine receptor interaction, metabolism of xenobiotics by cytochrome P450, and viral protein interaction with cytokine and cytokine receptor pathways. Most of the enriched pathways are metabolic pathways predominantly enriched by downregulated genes, suggesting numerous downregulated metabolic pathways in the high-risk group. Subsequent tumor immune infiltration analysis indicated that neutrophil infiltration, resting CD4 memory T cells, and resting natural killer (NK) cells correlated with the risk score. After verifying that the seven gene expression levels in different KRAS-mutated pancreatic cancer cell lines were similar to that in the model, we screened potential drugs related to the risk score.DiscussionThis study established, analyzed, and validated a model for predicting the prognosis of PDAC based on risk stratification according to KRAS mutations, and identified differential pathways and highly effective drugs.
TCRβ clones in muscle tissue share structural features in patients with idiopathic inflammatory myopathy and are associated with disease activity
Authors: Anang Dornatien C. , Walter Hannah A. W. , Lim Johan , Niewold Ilse T. G. , van der Weele Linda , Aronica Eleonora , Eftimov Filip , Raaphorst Joost , van Schaik Barbera D. C. , van Kampen Antoine H. C. , van der Kooi Anneke J. , de Vries Niek 
Published: January 2024

ObjectivesTo characterize the T cell receptor (TCRβ) repertoire in peripheral blood and muscle tissues of treatment naïve patients with newly diagnosed idiopathic inflammatory myopathies (IIMs).MethodsHigh throughput RNA sequencing of the TCRβ chain was performed in peripheral blood and muscle tissue in twenty newly-diagnosed treatment-naïve IIM patients (9 DM, 5 NM/OM, 5 IMNM and 1 ASyS) and healthy controls. Results thereof were correlated with markers of disease activity.ResultsMuscle tissue of IIM patients shows more expansion of TCRβ clones and decreased diversity when compared to peripheral blood of IIM as well as healthy controls (both p=0.0001). Several expanded TCRβ clones in muscle are tissue restricted and cannot be retrieved in peripheral blood. These clones have significantly longer CDR3 regions when compared to clones (also) found in circulation (p=0.0002), while their CDR3 region is more hydrophobic (p&lt;0.01). Network analysis shows that clonal TCRβ signatures are shared between patients. Increased clonal expansion in muscle tissue is significantly correlated with increased CK levels (p=0.03), while it tends to correlate with decreased muscle strength (p=0.08).ConclusionNetwork analysis of clones in muscle of IIM patients shows shared clusters of sequences across patients. Muscle-restricted CDR3 TCRβ clones show specific structural features in their T cell receptor. Our results indicate that clonal TCRβ expansion in muscle tissue might be associated with disease activity. Collectively, these findings support a role for specific clonal T cell responses in muscle tissue in the pathogenesis of the IIM subtypes studied.
Comparative transcriptomic insights into the domestication of Pleurotus abieticola for coniferous cultivation
Authors: Sun Lei , Sossah Frederick Leo , Li Yu , Sun Huiying 
Published: January 2024

Introduction:Pleurotus abieticola, a promising edible fungus in the Pleurotaceae family, especially its ability to utilize coniferous substrate, holds significant potential for commercial cultivation. However, few reports on the adaptation of P. abieticola to coniferous substrate from the perspective of omics.Methods: This study explores the biological characteristics, domestication process, and nutritional composition of P. abieticola, along with its adaptability to coniferous substrates using transcriptomics. We assessed biological characteristics, optimizing mycelial growth on agar medium with varied carbon and nitrogen sources, temperature, and pH. Additionally, the optimization process extended to fruiting bodies, where impact on the differentiation were evaluated under varying light conditions. Fruiting body nutrient composition was analyzed per the Chinese National Food Safety Standard. Transcriptome sequencing focused on P. abieticola mycelial colonized coniferous and broadleaved substrates.Results and Discussion: The optimal conditions for mycelial growth were identified: dextrin (carbon source), diammonium hydrogen phosphate (nitrogen source), 25°C (temperature), and pH 7.0. White light promoted fruiting body growth and differentiation. Larch substrate exhibited superior yield (190 g) and biological efficiency (38.0%) compared to oak (131 g, 26.2%) and spruce (166 g, 33.2%). P. abieticola showcased high dietary fiber, protein, and total sugar content, low fat, and sufficient microelements. Transcriptome analysis revealed significant key genes involved in lignocellulose degradation, stress-resistant metabolism, and endocytosis metabolism, underscoring their pivotal for coniferous adaptation. This study offers valuable insights for the commercial development and strain breeding of P. abieticola, efficiently leveraging conifer resources. The findings underscore its potential as a valuable source for food, medicinal products, and biotechnological applications.
Characterizing a new clinical phenotype: the co-existence of cerebral venous outflow and connective tissue disorders
Authors: Midtlien Jackson P. , Curry Brian P. , Chang Emily , Kiritsis Nicholas R. , Aldridge Jennifer B. , Fargen Kyle M. 
Published: January 2024

BackgroundThere is increasing recognition of connective tissue disorders and their influence on disease in the general population. A conserved clinical phenotype involving connective tissue disorders and idiopathic intracranial hypertension (IIH) and associated cerebral venous outflow disorders (CVD) has not been previously described.MethodsA single-institution retrospective review of a prospectively maintained database of patients with connective tissue disorders and CVD was performed.ResultsA total of 86 patients were identified. The majority of these patients carried a diagnosis of Ehlers-Danlos syndrome (55%) and most were non-obese (mean body mass index 29.7 kg/m2), Caucasian (90%) females (87%). Most prevalent presenting symptoms included pressure headache (98%), dizziness (90%), tinnitus (92%), and cognitive dysfunction (69%). Aside from CVD and IIH, the most common associated conditions were postural orthostatic tachycardia syndrome (POTS; 55.8%), cerebrospinal fluid (CSF) leaks (51.2%), dysautonomia (45.3%), cranio-cervical instability (37.2%), mast cell activation syndrome (25.6%), and tethered cord syndrome (23.3%). Allergies to medications (87.2%) and surgical tape (19.8%) were also frequent. Despite significantly lower opening pressures on lumbar puncture, headache severity and quality of life scores were reported with the same severity of classic IIH patients, suggesting an underlying hypersensitivity to intracranial pressures and cerebral venous congestion.ConclusionThere is a rare but conserved clinical phenotype that has not been described previously that presents with severe IIH symptoms in predominantly young, non-obese Caucasian women with a high associated incidence of dysautonomia, POTS, craniocervical instability, and CSF leaks, among others.
Association between body weight perception and intuitive eating among undergraduate students in China: the mediating role of body image
Authors: Zhu Yuanyuan , Gao Jiage , Gao Qinyi , Chen Dandan , Zeng Zhi 
Published: January 2024

BackgroundThe association between body weight perception and intuitive eating among undergraduate students in China remains insufficiently understood. In the present study, we were aimed to examine the correlation between body weight perception, body image, and intuitive eating and determine whether the link between body weight perception and intuitive eating was influenced by body image.MethodsA total of 1,050 undergraduate students completed the survey. Participants provided self-reported demographic details and completed two structured scales. The Body Esteem Scale for Adolescents and Adults (BESAA) and the Intuitive Eating Scale-2 (IES-2) were employed to assess body image and intuitive eating. Analysis of the mediation model was conducted using version 4.1 of the PROCESS Macro. Results with a value of p less than 0.05 were deemed statistically significant.ResultsThe average age of the participants was 20.08 years (SD = 1.64). Among the students, 837 (79.7%) were female, and 212 (20.3%) were male. Body image (r = −0.429, p &lt; 0.001) and intuitive eating (r = −0.313, p &lt; 0.001) exhibited significant negative associations with body weight perception. Furthermore, body image showed a significant positive correlation with intuitive eating (r = 0.318, p &lt; 0.001). Significant mediating effects of body image were identified concerning intuitive eating and body weight perception in the right weight (95% bootstrap CI = 0.007, 0.040) and overweight groups (95% bootstrap CI = −0.048, −0.009). The indirect effects of body image constituted 12.19% and 15.33% of the total effects of intuitive eating in these two groups.ConclusionAlthough the indirect effects were not substantial, these outcomes shed light on the partial understanding of how body weight perception impacted intuitive eating via body image. Importantly, our findings emphasized the significance of body image and body weight perception, offering a novel insight for prospective interventions targeting undergraduate students.