bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal relationships between susceptibility and severity of COVID-19 and neuromyelitis optica spectrum disorder (NMOSD) in European population: a bidirectional Mendelian randomized study
Authors: Wang Shengnan , Wang Lijuan , Wang Jianglong , Zhu Mingqin 
Published: December 2023

BackgroundNeurological disorders can be caused by viral infections. The association between viral infections and neuromyelitis optica spectrum disorder (NMOSD) has been well-documented for a long time, and this connection has recently come to attention with the occurrence of SARS-CoV-2 infection. However, the precise nature of the causal connection between NMOSD and COVID-19 infection remains uncertain.MethodsTo investigate the causal relationship between COVID-19 and NMOSD, we utilized a two-sample Mendelian randomization (MR) approach. This analysis was based on the most extensive and recent genome-wide association study (GWAS) that included SARS-CoV-2 infection data (122616 cases and 2475240 controls), hospitalized COVID-19 data (32519 cases and 2062805 controls), and data on severe respiratory confirmed COVID-19 cases (13769 cases and 1072442 controls). Additionally, we incorporated a GWAS meta-analysis comprising 132 cases of AQP4-IgG-seropositive NMOSD (NMO-IgG+), 83 cases of AQP4-IgG-seronegative NMOSD (NMO-IgG−), and 1244 controls.ResultsThe findings of our study indicate that the risk of developing NMO-IgG+ is elevated when there is a genetic predisposition to SARS-CoV-2 infection (OR = 5.512, 95% CI = 1.403-21.657, P = 0.014). Furthermore, patients with genetically predicted NMOSD did not exhibit any heightened susceptibility to SARS-CoV2 infection, COVID-19 hospitalization, or severity.Conclusionour study using Mendelian randomization (MR) revealed, for the first time, that the presence of genetically predicted SARS-CoV2 infection was identified as a contributing factor for NMO-IgG+ relapses.
AML alters bone marrow stromal cell osteogenic commitment via Notch signaling
Authors: Tomasoni Chiara , Arsuffi Corinne , Donsante Samantha , Corsi Alessandro , Riminucci Mara , Biondi Andrea , Pievani Alice , Serafini Marta 
Published: December 2023

IntroductionAcute myeloid leukemia (AML) is a highly heterogeneous malignancy caused by various genetic alterations and characterized by the accumulation of immature myeloid blasts in the bone marrow (BM). This abnormal growth of AML cells disrupts normal hematopoiesis and alters the BM microenvironment components, establishing a niche supportive of leukemogenesis. Bone marrow stromal cells (BMSCs) play a pivotal role in giving rise to essential elements of the BM niche, including adipocytes and osteogenic cells. Animal models have shown that the BM microenvironment is significantly remodeled by AML cells, which skew BMSCs toward an ineffective osteogenic differentiation with an accumulation of osteoprogenitors. However, little is known about the mechanisms by which AML cells affect osteogenesis.MethodsWe studied the effect of AML cells on the osteogenic commitment of normal BMSCs, using a 2D co-culture system.ResultsWe found that AML cell lines and primary blasts, but not normal hematopoietic CD34+ cells, induced in BMSCs an ineffective osteogenic commitment, with an increase of the early-osteogenic marker tissue non-specific alkaline phosphatase (TNAP) in the absence of the late-osteogenic gene up-regulation. Moreover, the direct interaction of AML cells and BMSCs was indispensable in influencing osteogenic differentiation. Mechanistic studies identified a role for AML-mediated Notch activation in BMSCs contributing to their ineffective osteogenic commitment. Inhibition of Notch using a γ-secretase inhibitor strongly influenced Notch signaling in BMSCs and abrogated the AML-induced TNAP up-regulation.DiscussionTogether, our data support the hypothesis that AML infiltration produces a leukemia-supportive pre-osteoblast-rich niche in the BM, which can be partially ascribed to AML-induced activation of Notch signaling in BMSCs.
Association of long-term aspirin use with kidney disease progression
Authors: Lu Jun Ling , Shrestha Prabin , Streja Elani , Kalantar-Zadeh Kamyar , Kovesdy Csaba P. 
Published: December 2023

BackgroundChronic microinflammation contributes to the progression of chronic kidney disease (CKD). Aspirin (ASA) has been used to treat inflammation for centuries. The effects of long-term low-dose ASA on CKD progression are unclear.MethodsWe examined the association of long-term use of newly initiated low-dose ASA (50–200 mg/day) with all-cause mortality using Cox proportional hazard models; with cardiovascular/cerebrovascular (CV) mortality and with end stage kidney disease (ESKD) using Fine and Gray competing risk regression models; with progression of CKD defined as patients’ eGFR slopes steeper than −5 mL/min/1.73m2/year using logistic regression models in a nationwide cohort of US Veterans with incident CKD. Among 831,963 patients, we identified 385,457 who either initiated ASA (N = 21,228) within 1 year of CKD diagnosis or never received ASA (N = 364,229). We used propensity score matching to account for differences in key characteristics, yielding 29,480 patients (14,740 in each group).ResultsIn the matched cohort, over a 4.9-year median follow-up period, 11,846 (40.2%) patients (6,017 vs. 5,829 ASA users vs. non-users) died with 25.8% CV deaths, and 934 (3.2%) patients (476 vs. 458) reached ESKD. ASA users had a higher risk of faster decline of kidney functions, i.e., steeper slopes (OR 1.30 [95%CI: 1.18, 1.44], p &lt; 0.01), but did not have apparent benefits on mortality (HR 0.97 [95%CI: 0.94, 1.01], p = 0.17), CV mortality (Sub-Hazard Ratio [SHR]1.06 [95%CI: 0.99–1.14], p = 0.11), or ESKD (SHR1.00 [95%CI: 0.88, 1.13], p = 0.95).ConclusionChronic low-dose ASA use was associated with faster kidney function deterioration, and no association was observed with mortality or risk of ESKD.
Effects of backward-directed resistance on propulsive force generation during split-belt treadmill walking in non-impaired individuals
Authors: Moradian Negar , Ko Mansoo , Hurt Christopher P. , Brown David A. 
Published: December 2023

IntroductionBackward-directed resistance is the resistance applied in the opposite direction of the individual’s walking motion. Progressive application of backward-directed resistance during walking at a target speed engages adaptive motor control to maintain that speed. During split-belt walking, a motor control strategy must be applied that allows the person to keep up with the two belts to maintain their position on the treadmill. This situation becomes more challenging when progressive resistance is applied since each limb needs to adapt to the greater resistance to maintain the position. We propose that strategies aimed at changing relative propulsion forces with each limb may explain the motor control strategy used. This study aimed to identify the changes in propulsive force dynamics that allow individuals to maintain their position while walking on an instrumented split-belt treadmill with progressively increasing backward-directed resistance.MethodsWe utilized an instrumented split-belt treadmill while users had to overcome a set of increasing backward-directed resistance through the center of mass. Eighteen non-impaired participants (mean age = 25.2 ± 2.51) walked against five levels of backward resistance (0, 5, 10, 15, and 20% of participant’s body weight) in two different modalities: single-belt vs. split-belt treadmill. On the single-belt mode, the treadmill’s pace was the participant’s comfortable walking speed (CWS). In split-belt mode, the dominant limb’s belt pace was half of the CWS, and the non-dominant limb’s belt speed was at the CWS.ResultsWe assessed differences between single-belt vs. split-belt conditions in the slope of the linear relationship between change in propulsive impulse relative to change of backward resistance amount. In split-belt conditions, the slower limb showed a significantly steeper increase in propulsion generation compared to the fast limb across resistance levels.DiscussionAs a possible explanation, the slow limb also exhibited a significantly increased slope of the change in trailing limb angle (TLA), which was strongly correlated to the propulsive impulse slope values. We conclude that the motor control strategy used to maintain position on a split-belt treadmill when challenged with backward-directed resistance is to increase the propulsive forces of the slow limb relative to the fast limb by progressively increasing the TLA.Clinical trial registrationClinicalTrials.gov, identifier NCT04877249.
Expert group recommendation on inhaled mucoactive drugs in pediatric respiratory diseases: an Indian perspective
Authors: Singh Meenu , Varkki Sneha , Kinimi Ilin , Das Rashmi R. , Goyal Jagdish Prasad , Bhat Mushtaq , Dayal Rajeshwar , Kalyan Pawan , Gairolla Jitender , Khosla Indu 
Published: December 2023

BackgroundCurrently, there are no guidelines or consensus statements about the usage of inhaled mucoactive drugs in pediatric respiratory disease conditions from an Indian perspective.ObjectiveTo develop a practical consensus document to help pediatricians in clinical decision-making when choosing an appropriate mucoactive drug for the management of specific respiratory disease conditions.MethodsA committee of nine experts with significant experience in pediatric respiratory disease conditions and a microbiological expert constituted the panel. An electronic search of the PubMed/MEDLINE, Cochrane Library, Scopus, and Embase databases was undertaken to identify relevant articles. Various combinations of keywords such as inhaled, nebulized, mucoactive, mucolytic, mucokinetic, expectorants, mucoregulators, mucociliary clearance, respiratory disorders, pediatric, cystic fibrosis (CF), non-CF bronchiectasis, acute wheezing, asthma, primary ciliary dyskinesia (PCD), critically ill, mechanical ventilation, tracheomalacia, tracheobronchomalacia, esophageal atresia (EA), tracheoesophageal fistula (TEF), acute bronchiolitis, sputum induction, guideline, and management were used. Twelve questions were drafted for discussion. A roundtable meeting of experts was conducted to arrive at a consensus. The level of evidence and class of recommendation were weighed and graded.ConclusionsInhaled mucoactive drugs (hypertonic saline, dry powder mannitol, and dornase alfa) can enhance mucociliary clearance in children with CF. Experts opined that hypertonic saline could be beneficial in non-CF bronchiectasis, acute bronchiolitis, and PCD. The current state of evidence is inadequate to support the use of inhaled mucoactive drugs in asthma, acute wheezing, tracheomalacia, tracheobronchomalacia, and EA with TEF.
Effects of different levels of dried onion powder on nutrient digestibility, biochemical parameters, and nitrogen balance in Wistar albino rats with induced hyperuricemia
Authors: Umer Muhammad , Nisa Mahr Un , Ahmad Nazir , Rahim Muhammad Abdul , Al-Asmari Fahad 
Published: December 2023

Introduction: Onions (Allium cepa L.) are excellent sources of bioactive compounds and phytochemicals such as allicin, quercetin, fisetin, and other sulfurous compounds. Therefore, our study aimed to investigate the effects of dried onion powder on growth performance, nitrogen balance, and biochemical parameters in Wistar albino rats with induced hyperuricemia.Methods: A total of 24 rats were randomly divided into four groups, with six in each group: HU (positive control) and HOT1, HOT2, and HOT3 groups, which received a diet containing onion powder at concentrations of 11.13, 14.84, and 18.61 g/100 g, respectively. Hyperuricemia was induced in rats by administering a new formulation intraperitoneally (250 mg/kg potassium oxonate) and orally (40 mg/kg potassium bromate) daily for 14 days. After confirmation of hyperuricemia induction, rats were fed with onion-treated diets with various concentrations of quercetin for 21 days.Results: Significant decreases (p ≤ 0.05) in serum uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total cholesterol, and low-density lipoprotein were observed. An increasing trend (p ≤ 0.05) in the levels of hemoglobin (Hb), white blood cell (WBC), red blood cell (RBC), and platelet count was observed. An improvement in the levels of serum high-density lipoprotein, triglycerides, blood urea nitrogen, serum creatinine, serum total protein and neutrophils, lymphocytes, and monocytes was observed. A positive progress (p ≤ 0.05) was observed in growth performance and nutrient digestibility.Conclusion: In conclusion, a significantly lower uric acid level was observed in rats fed with HOT2 diet. Based on the ratio of the surface area (human/rat), the best recommended dose of onion for the incidence and prevention of hyperuricemia is 189.95 g, corresponding to the dose of 204 mg/day of quercetin in humans.
AAPH-induced oxidative damage reduced anion exchanger 1 (SLC4A1/AE1) activity in human red blood cells: protective effect of an anthocyanin-rich extract
Authors: Remigante Alessia , Spinelli Sara , Patanè Giuseppe Tancredi , Barreca Davide , Straface Elisabetta , Gambardella Lucrezia , Bozzuto Giuseppina , Caruso Daniele , Falliti Giuseppe , Dossena Silvia , Marino Angela , Morabito Rossana 
Published: December 2023

Introduction: During their lifespan in the bloodstream, red blood cells (RBCs) are exposed to multiple stressors, including increased oxidative stress, which can affect their morphology and function, thereby contributing to disease.Aim: This investigation aimed to explore the cellular and molecular mechanisms related to oxidative stress underlying anion exchanger 1 activity (band 3, SLC4A1/AE1) in human RBCs. To achieve this aim, the relationship between RBC morphology and functional and metabolic activity has been explored. Moreover, the potential protective effect of an anthocyanin-enriched fraction extracted from Callistemon citrinus flowers was studied.Methods: Cellular morphology, parameters of oxidative stress, as well as the anion exchange capability of band 3 have been analyzed in RBCs treated for 1 h with 50 mM of the pro-oxidant 2,2′-azobis (2-methylpropionamide)-dihydrochloride (AAPH). Before or after the oxidative insult, subsets of cells were exposed to 0.01 μg/mL of an anthocyanin-enriched fraction for 1 h.Results: Exposure to AAPH caused oxidative stress, exhaustion of reduced glutathione, and over-activation of the endogenous antioxidant machinery, resulting in morphological alterations of RBCs, specifically the formation of acanthocytes, increased lipid peroxidation and oxidation of proteins, as well as abnormal distribution and hyper-phosphorylation of band 3. Expected, oxidative stress was also associated with a decreased band 3 ion transport activity and an increase of oxidized haemoglobin, which led to abnormal clustering of band 3. Exposure of cells to the anthocyanin-enriched fraction prior to, but not after, oxidative stress efficiently counteracted oxidative stress-related alterations. Importantly, protection of band3 function from oxidative stress could only be achieved in intact cells and not in RBC ghosts.Conclusion: These findings contribute a) to clarify oxidative stress-related physiological and biochemical alterations in human RBCs, b) propose anthocyanins as natural antioxidants to neutralize oxidative stress-related modifications, and 3) suggest that cell integrity, and therefore a cytosolic component, is required to reverse oxidative stress-related pathophysiological derangements in human mature RBCs.
The PPSQ: assessing parental, child, and partner’s playfulness in the preschool and early school years
Authors: Bureau Jean-François , Bandk Khachadour , Deneault Audrey-Ann , Turgeon Jessica , Seal Harshita , Brosseau-Liard Patricia 
Published: December 2023

IntroductionDevelopmental research has traditionally focused on parenting behaviors such as nurturance and care, due to a focus on mothers’ behaviors. Other parenting dimensions such as parental playfulness (i.e., use of creativity, imagination, and humor during parent–child interactions) have comparatively received little attention. Although some measures tap into parents’ and children’s playfulness, these measures are limited. Indeed, they do not assess multiple domains of playfulness (i.e., both parents’ and the child’s playfulness) or focus on one specific setting such as children’s play with peers. Additionally, existing measures do not consider parents’ reactions to their partners’ playfulness. To address this gap, we created the Playful Parenting Style Questionnaire (PPSQ), which assesses three domains of playfulness: (a) parental domain, (b) child domain, and (c) partner domain. The current study is part of a validation effort of the PPSQ using a quantitative design. We aimed to explore the structure of the PPSQ by conducting an exploratory factor analysis (EFA) for each domain of playfulness; and assess the construct validity of the PPSQ factors by examining the association between factors and existing measures of playful parenting, child playfulness, and co-parenting.MethodThe sample includes 347 parents (294 mothers and 53 fathers) of preschool/school-age children (M = 5.10 years; 182 girls, 127 boys). Parents were mostly White (76%) and from a low socioeconomic risk background. Parents completed a series of online questionnaires including the PPSQ, 3 existing measures of parent playfulness (Parental Playfulness Questionnaire; Adult Playfulness Scale; Challenging Parenting Behavior Scale), 2 existing measures of child playfulness (Child Behavior Inventory; Children’s Playfulness Scale), a coparenting instrument (Co-parenting Relationship Scale), and sociodemographic information.ResultsThe EFA revealed 4 factors for parental playfulness, 1 factor for child playfulness, and 3 factors for partner’s playfulness. The construct validity analyses identified multiple associations indicating convergence with existing measures for the parent and partners domain but not the child factor.DiscussionThis study allowed for a better understanding of the playful dynamics that occur within a family.
An assessment of appraisals of dating relationship conflicts and perceptions of appropriate coping strategies with psychologically abusive interactions
Authors: Matheson Kimberly , Wornovitzky Daphne , Landry Jyllenna , Anisman Hymie 
Published: December 2023

IntroductionStemming from a stress appraisal and coping perspective, the present investigation developed a methodology for assessing how individuals appraise abusive dating relationship conflicts (Study 1) and the implications of such appraisals for informing coping responses to abusive interactions (Study 2).MethodsParticipants ranging in age from 17 to 29 years (Study 1: 102 males, 339 females; Study 2: 88 males, 362 females) completed a survey in which they were presented with a series of 10 scenarios that conveyed relationship conflict cues that were ostensibly aligned with various forms of psychological abuse.ResultsFactor analyses indicated that blatant actions conducted in privacy were differentiated from more ambiguous public forms of psychological abuse, in that the latter were appraised by both males and females as more abusive. Females were further likely to appraise blatant conflicts as more threatening but at the same time more resolvable. Participants who had encountered abuse in their own intimate relationships were especially likely to appraise conflicts as abusive, threatening and uncontrollable. Such appraisals were associated with greater endorsement of avoidant coping strategies in response to an abusive encounter, irrespective of personal relationship experiences.DiscussionIt is suggested that how individuals appraise relationship conflicts may be key to their ability to cope effectively with such encounters or to provide appropriate support to those experiencing psychologically abusive relationships.
Case report: Anti N-methyl-D-aspartate autoimmune encephalitis following a mildly symptomatic COVID-19 infection in an adolescent male
Authors: Hainmueller Thomas , Lewis Lambert , Furer Tzvi 
Published: December 2023

BackgroundAntibodies against N-methyl-D-aspartate receptors are the most commonly identified cause of autoimmune encephalitis. While predominantly associated with malignancies, cases of anti-N-methyl-D-aspartate receptor autoimmune encephalitis have been reported after infections with the herpes-simplex virus or, more recently, in patients with severe COVID-19 disease.Case presentationA previously healthy 17-year-old male adolescent acutely developed psychosis with auditory and visual hallucinations, fluctuating mental status, and an isolated seizure 5 weeks after a mildly symptomatic COVID-19 infection. The symptoms continued to worsen, accompanied by catatonia, and additional neurological symptoms developed during the initial antipsychotic treatment. A diagnostic workup revealed antibodies against N-methyl-D-aspartate receptors in the cerebrospinal fluid without other major abnormalities. After establishing the diagnosis, initiation of immunomodulatory therapy stopped the symptom progression and led to full recovery within 2 months.ConclusionThe case is remarkable in that anti-N-methyl-D-aspartate receptor autoimmune encephalitis developed shortly after a COVID-19 infection in an adolescent, despite the individual experiencing only mild COVID symptoms. The diagnosis should be considered in cases of acute-onset psychotic symptoms during or after COVID-19 infection, particularly in individuals without a prior psychiatric history, who present with atypical psychiatric or neurological features.
Risk analysis of depression among adult patients with epilepsy of different sex: a retrospective single-center study from China
Authors: Guo Wang , Li Yu-xuan , Zhang Yi , Lv Xue-rui , Wang Sheng-xue , Zhang Shuang-yuan , Wang En-si , Chen Xin-jie , Li Yun 
Published: December 2023

ObjectiveTo determine sex differences in the prevalence of depression and assess the risk factors for depression among adult patients with epilepsy from the Dali area of China.MethodsWe retrospectively analyzed the clinical data of adult patients with epilepsy who visited the First Affiliated Hospital of Dali University from January 2017 to January 2022. Patient Health Questionnaire-9 was used to assess depressive symptoms in patients with epilepsy. The risk factors of depression were analyzed by binary logistic regression among different sex in patients with epilepsy.ResultsThere were significant sex differences in depression in patients with epilepsy (p &lt; 0.001), and females were 4.27 times more likely to suffer from depression than males (95% confidence interval: 3.70–4.92). The risk factors for depression among female patients with epilepsy included occupation (p &lt; 0.001), years with epilepsy (p &lt; 0.001), seizure frequency (p &lt; 0.001), seizure type (p &lt; 0.001), etiology (p &lt; 0.001), number of antiseizure medications used (p &lt; 0.001), antiseizure medications (p &lt; 0.001), and electroencephalogram findings (p &lt; 0.001). The risk factors for depression among male patients with epilepsy included age (p &lt; 0.001), ethnicity (p &lt; 0.001), occupation (p &lt; 0.001), years with epilepsy (p &lt; 0.001), seizure frequency (p &lt; 0.001), seizure type (p &lt; 0.001), etiology (p &lt; 0.001), number of antiseizure medications used (p &lt; 0.001), antiseizure medications (p &lt; 0.001), and electroencephalogram findings (p &lt; 0.001).ConclusionAdult female patients with epilepsy had a higher risk of depression than adult male patients with epilepsy. There were sex differences in the risk factors associated with depression among patients with epilepsy.