bluuebunny/crossref_metadata_2025_split huggingface dataset:
A scientometric visualization analysis of the gut microbiota and gestational diabetes mellitus
Authors: Su Zehao , Liu Lina , Zhang Jian , Guo Jingjing , Wang Guan , Zeng Xiaoxi 
Published: January 2025

BackgroundThe prevalence of gestational diabetes mellitus (GDM), a condition that is widespread globally, is increasing. The relationship between the gut microbiota and GDM has been a subject of research for nearly two decades, yet there has been no bibliometric analysis of this correlation. This study aimed to use bibliometrics to explore the relationship between the gut microbiota and GDM, highlighting emerging trends and current research hotspots in this field.ResultsA total of 394 papers were included in the analysis. China emerged as the preeminent nation in terms of the number of publications on the subject, with 128 papers (32.49%), whereas the United States had the most significant impact, with 4,874 citations. The University of Queensland emerged as the most prolific institution, contributing 18 publications. Marloes Dekker Nitert was the most active author with 16 publications, and Omry Koren garnered the most citations, totaling 154. The journal Nutrients published the most studies (28 publications, 7.11%), whereas PLoS One was the most commonly co-cited journal, with a total of 805 citations. With respect to keywords, research focuses can be divided into 4 clusters, namely, “the interrelationship between the gut microbiota and pregnancy, childbirth,” “the relationship between adverse metabolic outcomes and GDM,” “the gut microbiota composition and metabolic mechanisms” and “microbiota and ecological imbalance.” Key areas of focus include the interactions between the gut microbiota and individuals with GDM, as well as the formation and inheritance of the gut microbiota. Increasing attention has been given to the impact of probiotic supplementation on metabolism and pregnancy outcomes in GDM patients. Moreover, ongoing research is exploring the potential of the gut microbiota as a biomarker for GDM. These topics represent both current and future directions in this field.ConclusionThis study provides a comprehensive knowledge map of the gut microbiota and GDM, highlights key research areas, and outlines potential future directions.
Pediatric kidney transplant recipients are at an increased risk for dysbiosis
Authors: Yılmaz Gizem , Saygılı Seha , Ağbaş Ayşe , Karabağ Yılmaz Esra , Variş Ahmet , Canpolat Nur 
Published: January 2025

IntroductionThis study aimed to compare the gut microbiota composition in pediatric kidney transplant (KTx) recipient with that of healthy children.MethodsThis cross-sectional observational study included 30 pediatric KTx recipients aged between 7 and 21 years and 25 healthy children. The gut microbiota was assessed using 16S rRNA gene sequencing, with alpha and beta diversity, as well as all statistical analyses, conducted using the Phyloseq library in the R programming language. Taxonomic profiles were evaluated with QIIME2, and differences in gut microbiota profiles were compared using linear discriminant analysis effect size (LEFSe) with an LDA threshold of &gt;2 and p &lt; 0.05.ResultsNo significant differences were found in alpha and beta diversity between the KTx recipients and healthy controls. However, KTx recipients exhibited significant alterations in microbiota composition, including higher relative abundances of Verrucomicrobiota at the phylum level, and Akkermansia and Neisseria at the genus level (p &lt; 0.05 for all). Conversely, there was a decrease in bacterial genera belonging to the phylum Firmicutes. In addition, KTx recipients with a history of frequent urinary tract infections, diarrhea and reduced GFR showed significant increases in bacterial abundance (p &lt; 0.05 for all).DiscussionPediatric KTx recipients demonstrated significant alterarions in gut microbiota composition, indicating dysbiosis. Further studies are needed to elucidate the cause-and-effect relationships of these changes and their impact on clinical consequencies and long-term prognosis.
A novel two-component system contributing the catabolism of c-di-GMP influences virulence in Aeromonas veronii
Authors: Liu Chaolun , Shao Jia , Ma Xiang , Tang Yanqiong , Li Juanjuan , Li Hong , Chi Xue , Liu Zhu 
Published: January 2025

IntroductionResponse regulators from diverse two-component systems often function as diguanylate cyclases or phosphodiesterases, thereby enabling precise regulation of intracellular c-di-GMP levels to control bacterial virulence and motility. However, the regulatory mechanisms of c-di-GMP require further elucidation.MethodsThis study confirmed that ArrS and ArrR form a two-component system via structural analysis, two-hybrid, and phosphodiesterase activity detection. To evaluate the impact of ArrS/ArrR on intracellular c-di-GMP levels, biofilm detection, motility detection, fluorescence reporter plasmids, and LC-MS/MS analysis were employed. One-hybrid, EMSA, and RT-qPCR were used to demonstrate the function of ArgR on arrSR promoter. The roles of ArrS/ArrR in Aeromonas veronii were investigated using RT-qPCR, murine model, and proteomics.ResultsArrS and ArrR constituted a two-component system in Aeromonas veronii and were transcriptionally repressed by ArgR. ArrR exhibited phosphodiesterase activity, which is inhibited through phosphorylation mediated by ArrS. In Aeromonas veronii, ArrS/ArrR significantly altered the intracellular c-di-GMP levels. In a murine model, ΔarrS exhibited increased pathogenicity, leading to elevated TNF-α and IFN-γ levels in serum, and severer toxicity to spleen and kidney. These effects might be elucidated by the upregulated inflammation-associated proteins in ΔarrS. Moreover, the exonuclease RecB was also up-regulated in ΔarrS.DiscussionWe elucidated the regulatory mechanism of ArrS/ArrR on intracellular c-di-GMP levels and its impact on the virulence in Aeromonas veronii, and discussed the intricate relationship between c-di-GMP metabolism and arginine metabolism.
The effects of Bifidobacterium animalis QC08 on reducing uric acid level and providing renal protection in mice with hyperuricemia
Authors: Mao Huijia , Fan Yang , Tan Fang , Long Xingyao 
Published: January 2025

ObjectiveThe present study aimed to investigate the uric acid-lowering effects of Bifidobacterium animalis QC08 and explore its underlying mechanisms.MethodsHyperuricemia (HUA) model in mice was established using potassium oxonate (250 mg/kg) and yeast extract (15 g/kg). The serum levels of uric acid (UA), blood urea nitrogen (BUN), creatinine (Cr), and liver xanthine oxidase (XO) were measured in four groups, including normal group, control group, allopurinol group (5 mg/kg), and Bifidobacterium animalis QC08 group (1010 CFU/kg) using enzyme colorimetry. Additionally, serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were assessed using enzyme-linked immunosorbent assay (ELISA). Pathological changes in renal tissue were examined through hematoxylin–eosin (HE) staining.ResultsIn vivo experimental results indicated that compared with the normal group, the serum UA, Cr, and BUN levels, as well as the levels of inflammatory factors (TNF-α and IL-1β), and the activities of hepatic xanthine oxidase (XOD) and adenosine deaminase (ADA) were significantly elevated in the control group (p &lt; 0.05). The expression levels of uric acid transport-related genes (UAT, ABCG2, and OAT1) in kidney tissue were significantly downregulated (p &lt; 0.05), and evident kidney damage was found. In contrast, compared with the control group, the Bifidobacterium animalis QC08 group exhibited a significant decrease in serum UA, BUN, Cr, TNF-α, and IL-6 levels, along with reduced hepatic XOD and ADA activities (p &lt; 0.05). Additionally, Bifidobacterium animalis QC08 was found to regulate the mRNA transcription of renal uric acid transporters, leading to significantly upregulation of the expression levels of UAT, ABCG2, and OAT1 genes (p &lt; 0.05).ConclusionBifidobacterium animalis QC08 demonstrates certain uric acid-lowering, anti-inflammatory, and renal protective effects, which are associated with the inhibition of XOD activity and the modulation of the expression levels of uric acid transporter genes (UAT, ABCG2, and OAT1).
Sotorasib resistance triggers epithelial-mesenchymal transition and activates AKT and P38-mediated signaling
Authors: Megid Raquel Arantes , Ribeiro Guilherme Gomes , Gomes Izabela Natalia Faria , Laus Ana Carolina , Ferro Leal Letícia , Sussuchi da Silva Luciane , Ariwoola Abu-Bakr Adetayo , Dias Josiane Mourão , Reis Rui Manuel , Jose da Silva-Oliveira Renato 
Published: January 2025

BackgroundThe molecular non-genetic changes of resistance to sotorasib are currently uncertain. The aim of this study was to generate a sotorasib-resistant cell line via selective pressure and systematically examine the molecular and phenotypic alterations caused by resistance.MethodsMutant NCI-H358 (KRASG12C) were exposed to incremental doses (2–512 nM) of sotorasib. Then, resistant clones were separated by single-cell sorting. Proliferation was analyzed in real-time by xCELLigence; protein profiles were quantified by protein arrays; and mRNA expression profile was measured using the PanCancer Pathways panel by NanoString. In silico analyses were conducted from a database comprising patient-derived xenograft (PDX) models and cell lines resistant to sotorasib. AKT and p38. The synergistic effect of combining AKT, p38, and EGFR inhibitors was assessed using the SynergyFinder platform. Additionally, AKT and p38 genes were silenced using esiRNA.ResultsSotorasib-resistant H358-R cell line displayed markers of the mesenchymal-epithelial transition and loss of cell adhesion. Were identified 30 overexpressed genes in the resistance model, implicating in signaling pathways that leads to AKT activation and heightened protein expression levels of phosphorylated AKT and p38. To identify potential therapeutic strategies for overcoming sotorasib resistance, we investigated the combination of AKT and p38 inhibitors. Notably, combined inhibition of AKT (MK2206) and p38 (adezmapimod) restored sensitivity to sotorasib in resistant cell lines, as did silencing AKT expression.ConclusionThese findings underscore the importance of adaptive mechanisms in sotorasib resistance in NSCLC cells contributing by EMT activation and demonstrates synergic combination with AKT and p38 inhibitors to restore sotorasib sensitivity in KRASG12C cells.
Evidence from NHANES 2011–2014: a correlation between the weight-adjusted-waist index and cognitive abilities in the United States
Authors: Zhao Chan , Xu Xintian , Hao Chunyan 
Published: January 2025

BackgroundObesity exerts a significant detrimental impact on cognitive function. The weight-adjusted waist index (WWI) serves as a more precise indicator of visceral obesity that is independent of weight, in contrast to body mass index (BMI). Still, little research has been conducted on the interrelation between WWI and cognitive abilities. This investigation explored the link between WWI and older Americans’ cognitive function.MethodsUtilizing data from the 2011–2014 National Health and Nutrition Examination Cross-Sectional Survey, multiple linear regression analysis was used to assess the influence of WWI on cognitive abilities in those over 60. Three cognitive assessments were administered: the Animal Fluency Test (AFT), the Digit Symbol Substitution Test (DSST), and the Word Learning and Recall Module from the Coalition to Establish a Registry for Alzheimer’s Disease (CERAD). We implemented threshold effects analysis and smoothed curve fitting to elucidate the nonlinear relationship. Additionally, we analyzed subgroups to check for variability.ResultsThere were 2,762 participants, including1,504 (54.45%) females and 1,258 (45.55%) males. 53.77% of them had completed high school or above, and their average age was 69.05 ± 6.62. After controlling for confounding variables, the improved model predicted a negative connection between WWI and CERAD-Total, AFT, and DSST scores (all p &lt; 0.05). Furthermore, we performed a smoothenable curve fitting between WWI and cognitive function scores, resulting in a nonlinear connection and a threshold saturation effect. We also executed subgroup analyses and interaction tests based on gender, race, educational background, marital status, diabetes, body mass index, alcohol consumption, hypertension, smoking habits, stroke, depression, and sleep quality to assess whether the relationship between WWI and cognitive function was affected by heterogeneity across different population segments. The subgroup analysis found no significant differences in cognitive performance associated with WWI across the various subgroups.ConclusionHigher WWI levels are associated with impaired cognitive function in Americans aged 60 and older.
Area postrema syndrome in patients with autoimmune glial fibrillary acidic protein astrocytopathy
Authors: Li Qingchen , Teng Junfang 
Published: January 2025

ObjectiveArea postrema syndrome (APS) is a relatively rare symptom of autoimmune glial fibrillary acidic protein astrocytopathy (A-GFAP-A). This study aimed to report the APS in GFAP-immunoglobulin G (GFAP-IgG) positive patients.MethodsWe retrospectively analyzed the clinical data of APS in GFAP-IgG positive patients and reviewed relevant literature. Moreover, we compared these data with APS patients in aquaporin-4-IgG-positive neuromyelitis optica spectrum disorders (AQP4-IgG+ NMOSD).Results7 of 75 (9.3%) GFAP-IgG positive patients experienced APS, including 4 females and 3 males. The median age of onset was 42 years (range, 12–71 years). All patients presented with APS as their initial manifestation. Nausea and vomiting were observed in all 7 patients, while hiccups occurred in 5 patients. The median duration of APS episodes was 12 days (range, 6–40 days). None of the patients experienced isolated APS episodes during their illness. AQP4-IgG was positive in 2 patients. 5 patients had dorsal medulla oblongata lesions, while 3 patients showed an “inverted V” sign on axial images. In addition, 5 patients presented with longitudinally extensive linear or patchy lesions in cervical spinal cord extending to area postrema on sagittal images. All APS attacks completely disappeared after immunotherapy. Compared with the APS in AQP4 + NMOSD, APS in A-GFAP-A had a lower proportion of females (33.3% vs. 80%, p = 0.003), more hiccups (81% vs. 50%, p = 0.037), more leptomeningeal enhancement (61.9% vs. 5%, p = 0.000), higher CSF white blood cell count (median 120 vs. 10 cells/mm3, p = 0.000) and protein (median 0.949 vs. 0.407 g/L, p = 0.000). Furthermore, fewer patients with A-GFAP-A received long-term immunotherapy (19% vs. 65%, p = 0.003).ConclusionAPS often occurs as an initial manifestation of A-GFAP-A. MRI examination and antibody testing should be performed in suspected patients to avoid misdiagnosis.
Research status and trends of functional magnetic resonance imaging technology in the field of acupuncture: a bibliometric analysis over the past two decades
Authors: Wang Zhongke , Chen Lu , Jiang Tianyu , Zhang Qi , Zhao Jinying , Wang Fuchun 
Published: January 2025

ObjectiveAlthough fMRI has been widely used in the field of acupuncture. However, the literature analysis in this field still has significant differences. This study summarizes the current status of functional magnetic resonance imaging (fMRI) in the field of acupuncture and moxibustion and predicts its future trends through Web of Science bibliometric analysis.MethodsThis study uses “fMRI” and “acupuncture” as keywords to search for literature related to functional magnetic resonance imaging (fMRI) in acupuncture research in the Web of Science Core Collection database from January 1, 2004, to April 30, 2024. Visualization analyses were conducted using Citespace (version 6.3 R1) and VOSviewer (version 1.6.20). Citespace was employed to analyze annual publications, countries, institutions, keywords, and co-cited references. VOSviewer was used to analyze authors and co-cited authors, as well as journals and co-cited journals.ResultsFrom 2004 to 2024, a total of 967 publications were retrieved, of which 557 were included after screening. Despite annual fluctuations, the overall trend shows an increase. China and the Chinese Academy of Sciences are the countries and institutions with the highest number of publications, with Tian, J being the author with the most publications, and Kong, J having the highest Co-citation frequency. The article by Dhond, RP, published in 2008, has the highest Co-citation frequency among the co-cited literature. Evidence-based Complementary and Alternative Medicine is the journal with the most publications, while Neuroimage is the co-cited journal with the highest citation frequency. Keyword co-occurrence and burst reveal the main research hotspots, including the diversity of intervention methods, cortical activation, mechanisms related to pain-associated diseases, and brain-related diseases. Keyword burst detection reflects emerging trends, including meta-analysis and systematic reviews, the relationship between ischemic stroke and women, and the connection between mild cognitive impairment and prevention.ConclusionThis study employs bibliometric methods to explore the current status, research hotspots, and frontier issues regarding the application of functional magnetic resonance imaging (fMRI) technology in the field of acupuncture, providing new perspectives and directions for acupuncture fMRI research.
Effects of apple cider vinegar on glycemic control and insulin sensitivity in patients with type 2 diabetes: A GRADE-assessed systematic review and dose–response meta-analysis of controlled clinical trials
Authors: Arjmandfard Donya , Behzadi Mehrdad , Sohrabi Zahra , Mohammadi Sartang Mohsen 
Published: January 2025

Background and aimsDiabetes mellitus (DM) is a multifactorial metabolic disorder that affects the body’s ability to regulate blood sugar levels. Apple cider vinegar (ACV) could possibly improve diabetes; nevertheless, evidences provide conflicting results. This study aimed to evaluate the effects of ACV on glycemic profile in type 2 diabetes patients (T2DM) in controlled trials (CTs) by systematically reviewing and dose–response meta-analysis.MethodsThe Scopus, PubMed, and Web of Science databases were searched until November 2024 according to a systematic approach. All CTs investigating ACV’s effects on glycemic factors were included. We used a random-effects model to calculate WMDs and 95% confidence intervals (CIs). The present study assessed publication bias, sensitivity analysis, meta-regression, and heterogeneity based on standard methods. We assessed the bias risk of the included studies using Cochrane quality assessments and used GRADE (Grading of Recommendations Assessment, Development, and Evaluation) to calculate evidence certainty. We registered the study protocol at Prospero (no. CRD42023457493).ResultsOverall, we included seven studies in this meta-analysis. ACV significantly reduced fasting blood sugar (FBS) (WMD: −21.929 mg/dL, 95% CI: −29.19, −14.67, p &lt; 0.001) and HbA1c (WMD: −1.53, 95% CI: −2.65, −0.41, p = 0.008) and increased insulin (WMD: 2.059 μu/ml, 95% CI: 0.26, 3.86, p = 0.025), while it did not affect hemostatic model assessment for insulin resistance (HOMA-IR). We observed linear and non-linear associations between ACV consumption and FBS levels (p &lt; 0.001). Each 1 mL/day increase in ACV consumption was associated with a-1.255 mg/dL reduction in FBS. Moreover, greater effects on FBS were in dosages &gt;10.ConclusionACV had positive effects on FBS and HbA1c in T2DM patients.Systematic Review RegistrationThe study protocol was registered at Prospero (no. CRD42023457493).
Bifidobacterium breve BB05 alleviates depressive symptoms in mice via the AKT/mTOR pathway
Authors: Pan Yanni , Huang Qingling , Liang Yuan , Xie Yuwuqi , Tan Fang , Long Xingyao 
Published: January 2025

IntroductionThis study investigates the potential of Bifidobacterium breve BB05 (BB05) in mitigating depressive symptoms in a mouse model of Chronic Unpredictable Mild Stress (CUMS), with fluoxetine as a positive control.Methods and ResultsHigh-dose BB05 (1.0 × 109 CFU/kg, BB05H) significantly reduced anxiety- and depression-like behaviors in CUMS mice, as measured by the open field test, tail suspension test, and forced swim test. BB05 treatment also reduced pathological ileal damage, alleviated inflammation, and lowered serum levels of pro-inflammatory cytokines IL-6 and TNF-α. Additionally, BB05 increased serum 5-HT levels and decreased ACTH concentrations. Mechanistic analysis revealed that BB05 exerts antidepressant effects by activating the AKT/mTOR signaling pathway in the prefrontal cortex, promoting neuroprotection, neurogenesis, and synaptic plasticity.DiscussionThese findings suggest that BB05, particularly at higher doses, effectively alleviates CUMS-induced depressive behaviors and improves physiological outcomes, supporting the use of probiotics as a potential treatment for depression by targeting the AKT/mTOR pathway.
Prostate cancer incidence and mortality linked to metalworking fluid exposure: a systematic review and meta-analysis
Authors: Moradpour Zahra , Barik Amin , Jorjani Goljamal , Taherian Mohammad Reza , Tousizadeh Sepideh , Halimi Aram , Soleimani Yaser , Karimian Mobina , Khavari Tina , Kalankari Fateme Azizi , Asadipour Fatemeh , Azari Mojtaba , Shakouri Niloofar Yousefzadeh , Karamian Saeideh , Bahari Nasser , Mosavi Jarrahi Alireza 
Published: January 2025

BackgroundProstate cancer is the second most diagnosed cancer in men globally, with high prevalence in North America, Europe, and Australia. Occupational exposures, including metalworking fluids (MWFs), have emerged as a potential risk factor for prostate cancer, yet comprehensive studies on this association are limited.ObjectiveThis study aims to systematically review and conduct a meta-analysis to examine the incidence and mortality of prostate cancer linked to MWF exposure.MethodsA systematic review and meta-analysis were conducted following the PRISMA guidelines. A comprehensive search strategy was developed to identify relevant studies from PubMed, Scopus, Embase, and Web of Science. Inclusion criteria encompassed studies reporting on the association between MWF exposure and prostate cancer incidence or mortality. Data extraction and risk of bias assessment were performed independently by two reviewers, with discrepancies resolved by a third reviewer. Statistical analyses were conducted using STATA version 17.ResultsThe search identified 1376 unique references, with 5 studies meeting the inclusion criteria for the meta-analysis. These studies, conducted in the USA, primarily involved auto workers and reported Standardized Mortality Ratio (SMR), Relative Risk (RR) and 95% Confidence Interval (95%CI) measures. Meta-analysis revealed an overall RR of 1.06 (95% CI: 1.01-1.11) for prostate cancer incidence and an overall SMR of 1.20 (95% CI: 1.09-1.31) for prostate cancer mortality, indicating a statistically significant increased risk and mortality among MWF-exposed workers.ConclusionsThese findings carry significant implications for workplace safety regulations. Given the observed association between MWF exposure and prostate cancer risk, it is imperative to minimize occupational exposure through the implementation of effective engineering controls, personal protective equipment, and substitution of less hazardous fluids. Regular health surveillance and education programs for workers in industries utilizing MWFs are also essential to mitigate risk. Additionally, regulatory agencies should consider revising exposure limits and safety guidelines to account for emerging evidence on the carcinogenic potential of newer MWF formulations.