bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA
Authors: Moon Jeong , Liu Changchun 
Published: November 2023

AbstractNucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and deployable approach to molecular diagnostics. While exciting, there remain challenges limiting its practical applications, such as the need for pre-amplification and the lack of quantitative ability. Here, we develop an asymmetric CRISPR assay for cascade signal amplification detection of nucleic acids by leveraging the asymmetric trans-cleavage behavior of competitive crRNA. We discover that the competitive reaction between a full-sized crRNA and split crRNA for CRISPR-Cas12a can induce cascade signal amplification, significantly improving the target detection signal. In addition, we find that CRISPR-Cas12a can recognize fragmented RNA/DNA targets, enabling direct RNA detection by Cas12a. Based on these findings, we apply our asymmetric CRISPR assay to quantitatively detect microRNA without the need for pre-amplification, achieving a detection sensitivity of 856 aM. Moreover, using this method, we analyze and quantify miR-19a biomarker in plasma samples from bladder cancer patients. This asymmetric CRISPR assay has the potential to be widely applied for simple and sensitive nucleic acid detection in various diagnostic settings.
Critical role of electrons in the short lifetime of blue OLEDs
Authors: Kim Jaewook , Kim Joonghyuk , Kim Yongjun , Son Youngmok , Shin Youngsik , Bae Hye Jin , Kim Ji Whan , Nam Sungho , Jung Yongsik , Kim Hyeonsu , Kang Sungwoo , Jung Yoonsoo , Lee Kyunghoon , Choi Hyeonho , Kim Woo Youn 
Published: November 2023

AbstractDesigning robust blue organic light-emitting diodes is a long-standing challenge in the display industry. The highly energetic states of blue emitters cause various degradation paths, leading to collective luminance drops in a competitive manner. However, a key mechanism of the operational degradation of organic light-emitting diodes has yet to be elucidated. Here, we show that electron-induced degradation reactions play a critical role in the short lifetime of blue organic light-emitting diodes. Our control experiments demonstrate that the operational lifetime of a whole device can only be explained when excitons and electrons exist together. We examine the atomistic mechanisms of the electron-induced degradation reactions by analyzing their energetic profiles using computational methods. Mass spectrometric analysis of aged devices further confirm the key mechanisms. These results provide new insight into rational design of robust blue organic light-emitting diodes.
Strong transient magnetic fields induced by THz-driven plasmons in graphene disks
Authors: Han Jeong Woo , Sai Pavlo , But Dmytro B. , Uykur Ece , Winnerl Stephan , Kumar Gagan , Chin Matthew L. , Myers-Ward Rachael L. , Dejarld Matthew T. , Daniels Kevin M. , Murphy Thomas E. , Knap Wojciech , Mittendorff Martin 
Published: November 2023

AbstractStrong circularly polarized excitation opens up the possibility to generate and control effective magnetic fields in solid state systems, e.g., via the optical inverse Faraday effect or the phonon inverse Faraday effect. While these effects rely on material properties that can be tailored only to a limited degree, plasmonic resonances can be fully controlled by choosing proper dimensions and carrier concentrations. Plasmon resonances provide new degrees of freedom that can be used to tune or enhance the light-induced magnetic field in engineered metamaterials. Here we employ graphene disks to demonstrate light-induced transient magnetic fields from a plasmonic circular current with extremely high efficiency. The effective magnetic field at the plasmon resonance frequency of the graphene disks (3.5 THz) is evidenced by a strong ( ~ 1°) ultrafast Faraday rotation ( ~ 20 ps). In accordance with reference measurements and simulations, we estimated the strength of the induced magnetic field to be on the order of 0.7 T under a moderate pump fluence of about 440 nJ cm−2.
Biofilm heterogeneity-adaptive photoredox catalysis enables red light-triggered nitric oxide release for combating drug-resistant infections
Authors: Cheng Jian , Gan Guihai , Zheng Shaoqiu , Zhang Guoying , Zhu Chen , Liu Shiyong , Hu Jinming 
Published: November 2023

AbstractThe formation of biofilms is closely associated with persistent and chronic infections, and physiological heterogeneity such as pH and oxygen gradients renders biofilms highly resistant to conventional antibiotics. To date, effectively treating biofilm infections remains a significant challenge. Herein, we report the fabrication of micellar nanoparticles adapted to heterogeneous biofilm microenvironments, enabling nitric oxide (NO) release through two distinct photoredox catalysis mechanisms. The key design feature involves the use of tertiary amine (TA) moieties, which function as sacrificial agents to avoid the quenching of photocatalysts under normoxic and neutral pH conditions and proton acceptors at acidic pH to allow deep biofilm penetration. This biofilm-adaptive NO-releasing platform shows excellent antibiofilm activity against ciprofloxacin-resistant Pseudomonas aeruginosa (CRPA) biofilms both in vitro and in a mouse skin infection model, providing a strategy for combating biofilm heterogeneity and biofilm-related infections.
Stemness signature and targeted therapeutic drugs identification for Triple Negative Breast Cancer
Authors: Gul Samina , Pang Jianyu , Yuan Hongjun , Chen Yongzhi , yu Qian , Wang Hui , Tang Wenru 
Published: November 2023

AbstractTriple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and carries the worst prognosis, characterized by the lack of progesterone, estrogen, and HER2 gene expression. This study aimed to analyze cancer stemness-related gene signature to determine patients’ risk stratification and prognosis feature with TNBC. Here one-class logistic regression (OCLR) algorithm was applied to compute the stemness index of TNBC patients. Cox and LASSO regression analysis was performed on stemness-index related genes to establish 16 genes-based prognostic signature, and their predictive performance was verified in TCGA and METABERIC merged data cohort. We diagnosed the expression level of prognostic genes signature in the tumor immune microenvironment, analyzed the TNBC scRNA-seq GSE176078 dataset, and further validated the expression level of prognostic genes using the HPA database. Finally, the small molecular compounds targeted at the anti-tumor effect of predictive genes were screened by molecular docking; this novel stemness-based prognostic genes signature study could facilitate the prognosis of patients with TNBC and thus provide a feasible therapeutic target for TNBC.
Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer
Authors: Saridogan Turcin , Akcakanat Argun , Zhao Ming , Evans Kurt W. , Yuca Erkan , Scott Stephen , Kirby Bryce P. , Zheng Xiaofeng , Ha Min Jin , Chen Huiqin , Ng Patrick K. S. , DiPeri Timothy P. , Mills Gordon B. , Rodon Ahnert Jordi , Damodaran Senthil , Meric-Bernstam Funda 
Published: November 2023

AbstractSeveral alterations in fibroblast growth factor receptor (FGFR) genes have been found in breast cancer; however, they have not been well characterized as therapeutic targets. Futibatinib (TAS-120; Taiho) is a novel, selective, pan-FGFR inhibitor that inhibits FGFR1-4 at nanomolar concentrations. We sought to determine futibatinib’s efficacy in breast cancer models. Nine breast cancer patient–derived xenografts (PDXs) with various FGFR1-4 alterations and expression levels were treated with futibatinib. Antitumor efficacy was evaluated by change in tumor volume and time to tumor doubling. Alterations indicating sensitization to futibatinib in vivo were further characterized in vitro. FGFR gene expression between patient tumors and matching PDXs was significantly correlated; however, overall PDXs had higher FGFR3-4 expression. Futibatinib inhibited tumor growth in 3 of 9 PDXs, with tumor stabilization in an FGFR2-amplified model and prolonged regression (> 110 days) in an FGFR2 Y375C mutant/amplified model. FGFR2 overexpression and, to a greater extent, FGFR2 Y375C expression in MCF10A cells enhanced cell growth and sensitivity to futibatinib. Per institutional and public databases, FGFR2 mutations and amplifications had a population frequency of 1.1%–2.6% and 1.5%–2.5%, respectively, in breast cancer patients. FGFR2 alterations in breast cancer may represent infrequent but highly promising targets for futibatinib.
Spatio-temporal pattern evolution of China’s provincial tourism efficiency and development level based on DEA-MI model
Authors: Liao Zhenjie , Zhang Lijuan , Liang Shan 
Published: November 2023

AbstractThe spatial differences of efficiency and development level of regional tourism are evident, and the dynamic grasp of their spatiotemporal evolution characteristics and coupling coordination relationship is important to promote high-quality sustainable tourism development. Herein, we measured the tourism development level of 31 provincial units in China during 2000–2020, introduced the data envelopment analysis-based Malmquist productivity index to determine tourism efficiency, used exploratory spatiotemporal data analysis methods to explore the spatiotemporal dynamic characteristics of regional tourism differences and spatial structure, constructed a coupled coordination model of tourism efficiency and development level and analyzed their coupling excellence and synergistic consistency. The results were as follows: (1) The spatial differences of comprehensive tourism efficiency from 31 provinces were evident, and the average situation showed high and low distribution characteristics in the east and west, respectively. The interannual changes showed a fluctuating downward trend, with the scale efficiency playing a supporting role for comprehensive efficiency and technical efficiency playing an influencing and restraining role. (2) Less fluctuation existed in the local spatial structure of tourism efficiency and development level; the direction of dependence was more stable, tourism development level was slightly more volatile, and the spatial dependency direction changes were similar. The tourism efficiency of local structure competition posture was stronger than that of collaboration, with the tourism development level collaboration integration being stronger. (3) The local spatial structure of tourism development level was more stable, the relative position of provincial units was more difficult to change, tourism efficiency of local spatial structure was unstable, and provincial units exhibited a greater possibility of change. (4) Tourism efficiency and the scale of the overall coupling degree and coupling coordination gradually improved, with similar spatiotemporal values. The overall coupling degree and coupling coordination degree of tourism efficiency and development level gradually improved, with similar spatiotemporal heterogeneity and volatility of local evolution. The area with a higher value coupling coordination degree spread slower and more widely.
Dual pH- and temperature-responsive poly(dimethylaminoethyl methacrylate)-coated mesoporous silica nanoparticles as a smart drug delivery system
Authors: Ramezanian Sina , Moghaddas Jafarsadegh , Roghani-Mamaqani Hossein , Rezamand Azim 
Published: November 2023

AbstractA robust drug delivery system was created by grafting poly(dimethylaminoethyl methacrylate) (PDMAEMA) onto silica nanoparticles with two different lengths using an in situ atom transfer radical polymerization, resulting in the formation of a pH- and temperature-sensitive shell. The high molecular weight PDMAEMA demonstrated effective controlled drug release, and prevented drug release in healthy cells. Drug release occurred through polymer shell protonation at pH 5. The critical temperature of 41 °C facilitated rapid solvation of the shell polymers in the blood, preventing tissue accumulation and reducing toxicity compared to systems with lower critical solution temperatures. Field-emission scanning electron microscopy analysis and nitrogen adsorption/desorption analysis showed that the nanoparticles have a fine network, mesoporous structure, and a mean size of around 17 nm that show their excellent capacity for loading drugs. Fourier-transform infrared spectroscopy showed that all the modification steps and polymerization were successfully implemented. Thermogravimetric analysis showed PDMAEMA chains with two different lengths grafted onto the nanoparticles. Transmission electron microscopy analysis also showed grafted polymer chains on the hybrid nanoparticles. The release profile of model cancer drugs (doxorubicin and methotrexate) varied with pH and temperature, with high molecular weight PDMAEMA shells effectively preventing drug release at neutral pH. In vitro analysis using the HeLa cell line showed minimal toxicity in blank samples and significant release profile in acidic environment.
Temperature modulates the osmosensitivity of tilapia prolactin cells
Authors: Malintha G. H. T. , Woo Daniel W. , Celino-Brady Fritzie T. , Seale Andre P. 
Published: November 2023

AbstractIn euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different combinations of temperatures (20, 26 and 32 °C) and osmolalities (280, 330 and 420 mOsm/kg) for 6 h. Release of both Prl isoforms, Prl188 and Prl177, increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl188, but not Prl177, was suppressed at 20 °C. In SW fish, mRNA expression of prl188 increased with rising temperatures at lower osmolalities, while and prl177 decreased at 32 °C and higher osmolalities. In Prl cells of SW-acclimated tilapia incubated in hyperosmotic media, the expressions of Prl receptors, prlr1 and prlr2, and the stretch-activated Ca2+ channel, trpv4,decreased at 32 °C, suggesting the presence of a cellular mechanism to compensate for elevated Prl release. Transcription factors, pou1f1, pou2f1b, creb3l1, cebpb, stat3, stat1a and nfat1c, known to regulate prl188 and prl177, were also downregulated at 32 °C. Our findings provide evidence that osmoreception is modulated by temperature, and that both thermal and osmotic responses vary with acclimation salinity.
Reliability and validity of a widely-available AI tool for assessment of stress based on speech
Authors: Yawer Batul A. , Liss Julie , Berisha Visar 
Published: November 2023

AbstractCigna’s online stress management toolkit includes an AI-based tool that purports to evaluate a person’s psychological stress level based on analysis of their speech, the Cigna StressWaves Test (CSWT). In this study, we evaluate the claim that the CSWT is a “clinical grade” tool via an independent validation. The results suggest that the CSWT is not repeatable and has poor convergent validity; the public availability of the CSWT despite insufficient validation data highlights concerns regarding premature deployment of digital health tools for stress and anxiety management.
Automated analysis of feeding behaviors of females of the mosquito Aedes aegypti using a modified flyPAD system
Authors: Henriques-Santos Bianca Monteiro , Xiong Caixing , Pietrantonio Patricia V. 
Published: November 2023

AbstractMosquitoes present a global health challenge due to their ability to transmit human and animal pathogens upon biting and blood feeding. The investigation of tastants detected by mosquitoes and their associated feeding behaviors is needed to answer physiological and ecological questions that could lead to novel control methods. A high-throughput system originally developed for research in fruit flies feeding behavior, the flyPAD, was adapted and tested for behaviors associated with the interaction or consumption of liquid diets offered to females of the mosquito Aedes aegypti Liverpool strain. Females were given water, sucrose solution and sheep blood in choice and non-choice assays. The volume ingested was evaluated with fluorescein. The placement of the system on a heated surface allowed blood consumption, and without females puncturing a membrane. The flyPAD system recorded nine feeding behavioral variables, of which the number of sips and number of activity bouts correlated with meal volume ingested for both sucrose solution and blood. The adaptation to mosquitoes of the flyPAD system differentiated feeding behavior variables between two feeding deterrents, capsaicin, and caffeine. The flyPAD has potential to quickly assess diverse tastants in both sucrose and blood and may contribute to characterizing more precisely their mode of action.
A versatile model for lifetime of a component under stress
Authors: Almuhayfith Fatimah E. , Arslan Talha , Bakouch Hassan S. , Alnaghmosh Aminh M. 
Published: November 2023

AbstractIn this study, a versatile model, called $$\alpha$$
α
-monotone inverse Weibull distribution ($$\alpha$$
α
IW), for lifetime of a component under stress is introduced by using the $$\alpha$$
α
-monotone concept. The $$\alpha$$
α
IW distribution is also expressed as a scale-mixture between the inverse Weibull distribution and uniform distribution on (0, 1). The $$\alpha$$
α
IW distribution includes $$\alpha$$
α
-monotone inverse exponential and $$\alpha$$
α
-monotone inverse Rayleigh distributions as submodels and converenges to the inverse Weibull, inverse exponential, and inverse Rayleigh distributions as limiting cases. Also, slash Weibull, slash Rayleigh, and slash exponential distribuitons can be obtained under certain variable transformation and parameter settings. The $$\alpha$$
α
IW distribution is characterized by its hazard rate function and characterizing conditions are provided as well. Maximum likelihood, maximum product of spacing, and least squares methods are used to estimate distribution parameters. A Monte-Carlo simulation study is conducted to compare the efficiencies of the considered estimation methods. In the application part, two practical data sets, Kevlar 49/epoxy and Kevlar 373/epoxy, are modeled via the $$\alpha$$
α
IW distribution. Modeling performance of the $$\alpha$$
α
IW distribution is compared with its rivals by means of some well-known goodness-of-fit statistics and results show that $$\alpha$$
α
IW distribution performs better modeling than them. Results of comparison also indicate that obtaining the $$\alpha$$
α
IW distribution by using the $$\alpha$$
α
-monotone concept is cost effective since the new shape parameter added to the distribution by using the $$\alpha$$
α
-monotone concept significantly increases the modeling capability of the IW distribution. As a result of this study, it is shown that the $$\alpha$$
α
IW distribution can be an alternative to the well-known and recently-introduced distributions for modeling purposes.
Single-cell and bulk RNA sequencing reveals Anoikis related genes to guide prognosis and immunotherapy in osteosarcoma
Authors: Zhong Cheng , Yang Dongliang , Zhong Liping , Xie Weixing , Sun Guodong , Jin Daxiang , Li Yuming 
Published: November 2023

AbstractAnoikis resistance, a notable factor in osteosarcoma, plays a significant role in tumor invasion and metastasis. This study seeks to identify a distinct gene signature that is specifically associated with the anoikis subcluster in osteosarcoma. Clinical, single-cell, and transcriptional data from TARGET and GEO datasets were used to develop a gene signature for osteosarcoma based on the anoikis subcluster. Univariate Cox and LASSO regression analyses were employed. The signature's predictive value was evaluated using time-dependent ROC and Kaplan–Meier analyses. Functional enrichment analyses and drug sensitivity analyses were conducted. Validation of three modular genes was performed using RT-qPCR and Western blotting. Signature (ZNF583, CGNL1, CXCL13) was developed to predict overall survival in osteosarcoma patients, targeting the anoikis subcluster. The signature demonstrated good performance in external validation. Stratification based on the signature revealed significantly different prognoses. The signature was an independent prognostic factor. The low-risk group showed enhanced immune cell infiltration and improved immune function. Drug sensitivity analysis indicated efficacy of chemotherapy agents. Prognostic nomograms incorporating the signature provided greater predictive accuracy and clinical utility. Signatures related to the anoikis subcluster play a significant role in osteosarcoma progression. Incorporating these findings into clinical decision-making can improve osteosarcoma treatment and patient outcomes.
The effect of visual rivalry in peripheral head-mounted displays on mobility
Authors: Han Shui’er , Kim Sujin , Jung Jae-Hyun 
Published: November 2023

AbstractRecent head-mounted displays and smart glasses use vision multiplexing, an optical approach where two or more views are superimposed on each other. In vision multiplexing, augmented information is presented over an observer’s natural field of view, providing field expansion and critical information during mobility situations like walking and driving. Yet despite its utility, vision multiplexing may produce visual rivalry, a phenomenon where perception alternates between the augmented information and the background scene for seconds at a time. To investigate, we compared the effect of different peripheral vision multiplexing configurations (unilateral opaque, unilateral see-through and bilateral see-through) on the detection of augmented information, incorporating at the same time real-world characteristics (target eccentricity, depth condition, and gaze movement) for a more realistic assessment. Results showed a persistently lower target detection rate in unilateral configurations than the bilateral configuration, suggesting a larger effect of binocular rivalry on target visibility. Nevertheless, this effect does become attenuated when more naturalistic elements are incorporated, and we discuss recommendations for vision multiplexing design and possible avenues for further research.
CT-based comparison of porcine, ovine, and human pulmonary arterial morphometry
Authors: Goubergrits Leonid , Schafstedde Marie , Cesarovic Nikola , Szengel Angelika , Schmitt Boris , Wiegand Moritz , Romberg Jan , Arndt Andreas , Kuehne Titus , Brüning Jan 
Published: November 2023

AbstractTo facilitate pre-clinical animal and in-silico clinical trials for implantable pulmonary artery pressure sensors, understanding the respective species pulmonary arteries (PA) anatomy is important. Thus, morphological parameters describing PA of pigs and sheep, which are common animal models, were compared with humans. Retrospective computed tomography data of 41 domestic pigs (82.6 ± 18.8 kg), 14 sheep (49.1 ± 6.9 kg), and 49 patients (76.8 ± 18.2 kg) were used for reconstruction of the subject-specific PA anatomy. 3D surface geometries including main, left, and right PA as well as LPA and RPA side branches were manually reconstructed. Then, specific geometric parameters (length, diameters, taper, bifurcation angle, curvature, and cross-section enlargement) affecting device implantation and post-interventional device effect and efficacy were automatically calculated. For both animal models, significant differences to the human anatomy for most geometric parameters were found, even though the respective parameters’ distributions also featured relevant overlap. Out of the two animal models, sheep seem to be better suitable for a preclinical study when considering only PA morphology. Reconstructed geometries are provided as open data for future studies. These findings support planning of preclinical studies and will help to evaluate the results of animal trials.