bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term application of agronomic management strategies effects on soil organic carbon, energy budgeting, and carbon footprint under rice–wheat cropping system
Authors: Naresh R. K. , Singh P. K. , Bhatt Rajan , Chandra Mandapelli Sharath , Kumar Yogesh , Mahajan N. C. , Gupta S. K. , Al-Ansari Nadhir , Mattar Mohamed A. 
Published: January 2024

AbstractIn the plains of western North India, traditional rice and wheat cropping systems (RWCS) consume a significant amount of energy and carbon. In order to assess the long-term energy budgets, ecological footprint, and greenhouse gas (GHG) pollutants from RWCS with residual management techniques, field research was conducted which consisted of fourteen treatments that combined various tillage techniques, fertilization methods, and whether or not straw return was present in randomized block design. By altering the formation of aggregates and the distribution of carbon within them, tillage techniques can affect the dynamics of organic carbon in soil and soil microbial activity. The stability of large macro-aggregates (> 2 mm), small macro-aggregates (2.0–2.25 mm), and micro-aggregates in the topsoil were improved by 35.18%, 33.52%, and 25.10%, respectively, over conventional tillage (0–20 cm) using tillage strategies for conservation methods (no-till in conjunction with straw return and organic fertilizers). The subsoil (20–40 cm) displayed the same pattern. In contrast to conventional tilling with no straw returns, macro-aggregates of all sizes and micro-aggregates increased by 24.52%, 28.48%, and 18.12%, respectively, when conservation tillage with organic and chemical fertilizers was used. The straw return (aggregate-associated C) also resulted in a significant increase in aggregate-associated carbon. When zero tillage was paired with straw return, chemical, and organic fertilizers, the topsoil's overall aggregate-associated C across all aggregate proportions increased. Conversely, conventional tillage, in contrast to conservation tillage, included straw return as well as chemical and organic fertilizers and had high aggregate-associated C in the subsurface. This study finds that tillage techniques could change the dynamics of microbial biomass in soils and organic soil carbon by altering the aggregate and distribution of C therein.
Survival of microorganisms during two-year exposure in outer space near the ISS
Authors: Deshevaya Elena A. , Fialkina Svetlana V. , Shubralova Elena V. , Tsygankov Oleg S. , Khamidullina Natalia M. , Vasilyak Leonid M. , Pecherkin Vladimir Ya. , Shcherbakova Viktoria A. , Nosovsky Andrey M. , Orlov Оleg I. 
Published: January 2024

AbstractResults of an experiment named "Test" on survival and variability of microorganisms in open space near the International Space Station are presented. It was found after two-years exposure, spore-forming bacteria of the species Bacillus subtilis, fungi of the species Aureobasidium pullulans and archaea of the species Methanosarcina mazei S-6T, deposited on cotton wool, are able to survive, and their numbers decreased equally, regardless of whether the microorganisms belong to different taxonomic groups. The main factors for the long-term survival could be the result of their dehydration and partial lyophilization in the vacuum of near-Earth space. For the first time, after being in outer space, cyst-like cells of the archaea strain M. mazeiS-6T and a 14-day delay in their growth were detected when cultured on a nutrient medium compared to the ground-based control strain. In 30% of fungi species strains A. pullulans, isolated after a two-year stay in outer space, the resistance to γ—radiation increased compared to the control strain. It was found that the reaction to the action of various concentrations of hydrogen peroxide and 1% chlorine in the surviving strains of the fungus A. pullulans on the ISS is less pronounced than in the control strain.
Analysis of deafness susceptibility gene of neonates in northern Guangdong, China
Authors: Ma Zhanzhong , Huang Wenbo , Xu Jing , Qiu Jianwu , Liu Yulan , Ye Meixian , Fan Shushu 
Published: January 2024

AbstractThis study aimed to explore the molecular epidemiology characteristics of deafness susceptibility genes in neonates in northern Guangdong and provide a scientific basis for deafness prevention and control. A total of 10,183 neonates were recruited between January 2018 and December 2022 at Yuebei People's Hospital. Among these, a PCR hybridization screening group of 8276 neonates was tested for four deafness genes: GJB2, SLC26A4, mtDNA, and GJB3 by PCR hybridization. Another group used next-generation sequencing (NGS) to detect genetic susceptibility genes in 1907 neonates. In PCR hybridization screening group, 346 (4.18%) of 8276 neonates were found to be carriers of the deafness gene. Among these, 182 (2.2%) had GJB2 variants, 114 (1.38%) had SLC26A4 variants, 35 (0.42%) had mtDNA variants, and 15 (0.18%) had GJB3 variants. In NGS Screening Group, 195 out of 1907 neonates were found to be carriers of the deafness gene, with a positive rate of 10.22%. Among these, 137 (7.18%) had GJB2 variants, 41 (2.15%) had SLC26A4 variants, 11 (0.58%) had mtDNA variants, and 6 (0.31%) had GJB3 variants. The prevalence of deafness gene variants was high in Northern Guangdong Province. The most common gene for deafness was GJB2, followed by SLC26A4 and mtDNA. GJB3 variants are rare. Compared with PCR hybridization method, NGS technology can expand the screening scope and greatly improve the detection rate of deafness genes. The c.109G>A of GJB2 was found to occur at a high frequency, which should be considered. Therefore, it is important to conduct neonatal deafness gene screening to prevent and control hereditary deafness.
The impact of the digital economy on urban total factor productivity: mechanisms and spatial spillover effects
Authors: Zou Shaohui , Liao Zhe , Fan Xiangbo 
Published: January 2024

AbstractDigital economy is the indispensable pathway for driving industrial structural upgrading and fostering innovation and entrepreneurship in cities, ultimately facilitating China's economic transformation. Simultaneously, the enhancement of urban total factor productivity (TFP) serves as a crucial means to achieve high-quality economic development in cities. This study examines the specific impact of the digital economy on urban TFP using a panel data model with a sample of 285 Chinese prefecture-level cities from 2011 to 2019. Additionally, it employs a mediation effect model to test the mechanisms through which the digital economy influences urban TFP and utilizes a spatial Durbin model to analyze the spatial spillover effects of the digital economy on urban TFP. The research findings reveal the following key points: (1)The digital economy has an overall significantly positive impact on urban TFP. (2)The digital economy indirectly promotes urban TFP by encouraging the advancement of industrial structure and fostering innovation and entrepreneurship in cities. (3)The influence of the digital economy on urban TFP exhibits spatial spillover effects, where the digital economy in neighboring cities significantly enhances the TFP growth of local cities.The results of this study contribute to elucidating the mechanistic pathways through which the digital economy affects urban TFP, holding significant practical implications for achieving high-quality economic development in urban areas.
Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets
Authors: Pries Ralph , Kosyna Friederike Katharina , Depping Reinhard , Plötze-Martin Kirstin , Lange Christian , Meyhöfer Svenja , Meyhöfer Sebastian M. , Marquardt Jens U. , Bruchhage Karl-Ludwig , Steffen Armin 
Published: January 2024

AbstractObstructive sleep apnea syndrome (OSAS) and obesity go hand in hand in the majority of patients and both are associated with a systemic inflammation, immune disturbance and comorbidities such as cardiovascular disease. However, the unambiguous impact of OSAS and obesity on the individual inflammatory microenvironment and the immunological consequences of human monocytes has not been distinguished yet. Therefore, aim of this study was to investigate the impact of OSAS and obesity related factors on the inflammatory microenvironment by performing flow cytometric whole blood measurements of CD14/CD16 monocyte subsets in normal weight OSAS patients, patients with obesity but without OSAS, and patients with OSAS and obesity, compared to healthy donors. Moreover, explicitly OSAS and obesity related plasma levels of inflammatory mediators adiponectin, leptin, lipocalin and metalloproteinase-9 were determined and the influence of different OSAS and obesity related factors on cytokine secretion and expression of different adhesion molecules by THP-1 monocytes was analysed. Our data revealed a significant redistribution of circulating classical and intermediate monocytes in all three patient cohorts, but differential effects in terms of monocytic adhesion molecules CD11a, CD11b, CD11c, CX3CR1, CD29, CD49d, and plasma cytokine levels. These data were reflected by differential effects of OSAS and obesity related factors leptin, TNFα and hypoxia on THP-1 cytokine secretion patterns and expression of adhesion molecules CD11b and CD49d. In summary, our data revealed differential effects of OSAS and obesity, which underlines the need for a customized therapeutic regimen with respect to the individual weighting of these overlapping diseases.
Impact on the time elapsed since SARS-CoV-2 infection, vaccination history, and number of doses, on protection against reinfection
Authors: Sánchez-de Prada Laura , Martínez-García Ana María , González-Fernández Belén , Gutiérrez-Ballesteros Javier , Rojo-Rello Silvia , Garcinuño-Pérez Sonsoles , Álvaro-Meca Alejandro , Ortiz De Lejarazu Raúl , Sanz-Muñoz Iván , Eiros José M. 
Published: January 2024

AbstractSARS-CoV-2 reinfections have been frequent, even among those vaccinated. The aim of this study is to know if hybrid immunity (infection + vaccination) is affected by the moment of vaccination and number of doses received. We conducted a retrospective study in 746 patients with a history of COVID-19 reinfection and recovered the dates of infection and reinfection and vaccination status (date and number of doses). To assess differences in the time to reinfection(tRI) between unvaccinated, vaccinated before 6 months, and later; and comparing one, two or three doses (incomplete, complete and booster regime) we performed the log-rank test of the cumulative incidence calculated as 1 minus the Kaplan–Meier estimator. Also, an adjusted Cox-regression was performed to evaluate the risk of reinfection in all groups. The tRI was significantly higher in those vaccinated vs. non-vaccinated (p < 0.001). However, an early incomplete regime protects similar time than not receiving a vaccine. Vaccination before 6 months after infection showed a lower tRI compared to those vaccinated later with the same regime (adj-p < 0.001). Actually, early vaccination with complete and booster regimes provided lower length of protection compared to vaccinating later with incomplete and complete regime, respectively. Vaccination with complete and booster regimes significantly increases the tRI (adj-p < 0.001). Vaccination increases the time it takes for a person to become reinfected with SARS-CoV-2. Increasing the time from infection to vaccination increases the time in which a person could be reinfected and reduces the risk of reinfection, especially in complete and booster regimes. Those results emphasize the role of vaccines and boosters during the pandemic and can guide strategies on future vaccination policy.
Safety and tissue remodeling assay of small intestinal submucosa meshes using a modified porcine surgical hernia model
Authors: Liu Chenghu , Lin Zhenhua , Ruan Wenting , Gai Xiaoxiao , Qu Qiujin , Wang Changbin , Zhu Fuyu , Sun Xiaoxia , Zhang Jian 
Published: January 2024

AbstractIn studies to date, meshes based on extracellular matrix (ECM) have been extensively used in clinical applications. Unfortunately, little is known about the function of the immunogenic residual, absorbable profile during the tissue repair process. Moreover, there needs to be a recognized preclinical animal model to investigate the safety and efficacy of extracellular matrix meshes. Herein, we designed and fabricated a kind of SIS mesh followed by a scanned electron micrograph characterization and tested α-Gal antigen clearance rate and DNA residual. In order to prove the biocompatibility of the SIS mesh, cell viability, chemotaxis assay and local tissue reaction were assessed by MTT and RTCA cytotoxicity test in vitro as well as implantation and degradation experiments in vivo. Furthermore, we developed a stable preclinical animal model in the porcine ventral hernia repair investigation, which using laparoscopic plus open hybridization method to evaluate tissue adhesion, explant mechanical performance, and histologic analysis after mesh implantation. More importantly, we established a semi-quantitative scoring system to examine the ECM degradation, tissue remodeling and regeneration in the modified porcine surgical hernia model for the first time. Our results highlight the application prospect of the improved porcine ventral hernia model for the safety and efficacy investigation of hernia repair meshes.
Predicting m-health acceptance from the perspective of unified theory of acceptance and use of technology
Authors: Yang Marvello , Al Mamun Abdullah , Gao Jingzu , Rahman Muhammad Khalilur , Salameh Anas A. , Alam Syed Shah 
Published: January 2024

AbstractAddressing the growing popularity of mobile health (m-Health) technology in the health industry, the current study examined consumers’ intention and behaviour related to the usage of digital applications based on the unified theory of acceptance and use of technology (UTAUT). In particular, this study quantitatively assessed the moderating role of perceived product value and mediating role of intention to use m-Health application among Indonesians. This study adopted a cross-sectional design and collected quantitative data from conveniently selected respondents through an online survey, which involved 2068 Telegram users in Indonesia. All data were subjected to the analysis of partial least square- structural equation modeling (PLS-SEM). The obtained results demonstrated the moderating effect of perceived product value on the relationship between intention to use m-Health application (m-health app) and actual usage of m-Health app and the mediating effects of intention to use m-Health app on the relationships of perceived critical mass, perceived usefulness, perceived convenience, perceived technology accuracy, and perceived privacy protection on actual usage of m-Health app. However, the intention to use m-Health app did not mediate the influence of health consciousness and health motivation on the actual usage of m-Health app. Overall, this study’s findings on the significance of intention to use m-Health app and perceived product value based on the UTAUT framework serve as insightful guideline to expand the usage of m-Health app among consumers.
Daily supplement of sesame oil prevents postmenopausal osteoporosis via maintaining serum estrogen and aromatase levels in rats
Authors: Hsu Che-Chia , Ko Po-Yen , Kwan Ting-Hsien , Liu Ming-Yie , Jou I.-Ming , Lin Chi-Wei , Wu Po-Ting 
Published: January 2024

AbstractEstrogen deficiency is one of the main causes of postmenopausal osteoporosis in elderly women. Hormone replacement therapy has been employed to manage postmenopausal osteoporosis; however, it has raised concerns related to heart attacks and breast cancer. Sesame oil has been reported to affect sex hormone status. The aim of the present study is to evaluate the effect of sesame oil supplement on postmenopausal osteoporosis in rats. We used female Sprague Dawley rats that underwent bilaterally ovariectomy (OVX) as an experimental postmenopausal osteoporosis animal model. These rats were orally administrated sesame oil (0.25 or 0.5 mL/kg/day) for four months as the therapeutic group. We assessed bone mineral density (BMD) and the levels of osteocalcin, procollagen-I C-terminal propeptide (PICP), collagen cross-linked N-telopeptide (NTx), estradiol, and aromatase in the sera. The daily supplementation of sesame oil significantly increased BMD, serum osteocalcin levels, and trabecular areas in the OVX-treated rats. Sesame oil also elevated serum PICP levels and decreased NTx levels in these rats. Furthermore, sesame oil effectively maintained serum estradiol and aromatase levels in the OVX-induced osteoporosis rats. In conclusion, daily supplementation of sesame oil prevents postmenopausal osteoporosis by maintaining serum estrogen and aromatase levels, while also modulating the imbalance between bone formation and resorption in osteoporosis rats.
Identification of predilection sites for wild boar carcass search based on spatial analysis of Latvian ASF surveillance data
Authors: Rogoll Lisa , Schulz Katja , Staubach Christoph , Oļševskis Edvīns , Seržants Mārtiņš , Lamberga Kristīne , Conraths Franz Josef , Sauter-Louis Carola 
Published: January 2024

AbstractTargeted search for wild boar carcasses is essential for successful control of African swine fever (ASF) in wild boar populations. To examine whether landscape conditions influence the probability of finding ASF-positive carcasses, this study analyzed Global Positioning System (GPS) coordinates of Latvian wild boar carcasses and hunted wild boar, extracted from the CSF/ASF wild boar surveillance database of the European Union, and random coordinates in Latvia. Geographic information system (GIS) software was used to determine the landscape type and landscape composition of carcass detection sites and to measure distances from the carcasses to nearest waterbodies, forest edges, roads and settlements. The results of the automated measurements were validated by manually analyzing a smaller sample. Wild boar carcasses were found predominantly in forested areas and closer to waterbodies and forest edges than random GPS coordinates in Latvia. Carcasses of ASF-infected wild boar were found more frequently in transitional zones between forest and woodland shrub, and at greater distances from roads and settlements compared to ASF-negative carcasses and random points. This leads to the hypothesis, that ASF-infected animals seek shelter in quiet areas further away from human disturbance. A detailed collection of information on the environment surrounding carcass detection sites is needed to characterize predilection sites more accurately.
Exploring adsorption capacity and mechanisms involved in cadmium removal from aqueous solutions by biochar derived from euhalophyte
Authors: Ge Shaoqing , Zhao Shuai , Wang Lei , Zhao Zhenyong , Wang Shoule , Tian Changyan 
Published: January 2024

AbstractBiochar has shown potential as a sorbent for reducing Cd levels in water. Euhalophytes, which thrive in saline-alkali soils containing high concentrations of metal ions and anions, present an intriguing opportunity for producing biochar with inherent metal adsorption properties. This study focused on biochar derived from the euhalophyte Salicornia europaea and aimed to investigate its Cd adsorption capacity through adsorption kinetics and isotherm experiments. The results demonstrated that S. europaea biochar exhibited a high specific surface area, substantial base cation content, and a low negative surface charge, making it a highly effective adsorbent for Cd. The adsorption data fit well with the Langmuir isotherm model, revealing a maximum adsorption capacity of 108.54 mg g−1 at 25 °C. The adsorption process involved both surface adsorption and intraparticle diffusion. The Cd adsorption mechanism on the biochar encompassed precipitation, ion exchange, functional group complexation, and cation-π interactions. Notably, the precipitation of Cd2+ with CO32− in the biochar played a dominant role, accounting for 73.7% of the overall removal mechanism. These findings underscore the potential of euhalophytes such as S. europaea as a promising solution for remediating Cd contamination in aquatic environments.
Proline-mediated redox regulation in wheat for mitigating nickel-induced stress and soil decontamination
Authors: Atta Nimra , Shahbaz Muhammad , Farhat Fozia , Maqsood Muhammad Faisal , Zulfiqar Usman , Naz Nargis , Ahmed Muhammad Mahmood , Hassan Naveed Ul , Mujahid Nazoora , Mustafa Abd El-Zaher M. A. , Elshikh Mohamed S. , Chaudhary Talha 
Published: January 2024

AbstractNickel (Ni) is known as a plant micronutrient and serves as a component of many significant enzymes, however, it can be extremely toxic to plants when present in excess concentration. Scientists are looking for natural compounds that can influence the development processes of plants. Therefore, it was decided to use proline as a protective agent against Ni toxicity. Proline (Pro) is a popularly known osmoprotectant to regulate the biomass and developmental processes of plants under a variety of environmental stresses, but its role in the modulation of Ni-induced toxicity in wheat is very little explored. This investigation indicated the role of exogenously applied proline (10 mM) on two wheat varieties (V1 = Punjab-11, V2 = Ghazi-11) exposed to Ni (100 mg/kg) stress. Proline mediated a positive rejoinder on morphological, photosynthetic indices, antioxidant enzymes, oxidative stress markers, ion uptake were analyzed with and without Ni stress. Proline alone and in combination with Ni improved the growth, photosynthetic performance, and antioxidant capacity of wheat plants. However, Ni application alone exhibited strong oxidative damage through increased H2O2 (V1 = 28.96, V2 = 55.20) accumulation, lipid peroxidation (V1 = 26.09, V2 = 38.26%), and reduced translocation of macronutrients from root to shoot. Application of Pro to Ni-stressed wheat plants enhanced actions of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and total soluble protein (TSP) contents by 45.70, 44.06, 43.40, and 25.11% in V1, and 39.32, 46.46, 42.22, 55.29% in V2, compared to control plants. The upregulation of antioxidant enzymes, proline accumulation, and uptake of essential mineral ions has maintained the equilibrium of Ni in both wheat cultivars, indicating Ni detoxification. This trial insight into an awareness that foliar application of proline can be utilized as a potent biochemical method in mitigating Ni-induced stress and might serve as a strong remedial technique for the decontamination of polluted soil particularly with metals.
RNF31 promotes proliferation and invasion of hepatocellular carcinoma via nuclear factor kappaB activation
Authors: Hoshino Kouki , Nakazawa Seshiru , Yokobori Takehiko , Hagiwara Kei , Ishii Norihiro , Tsukagoshi Mariko , Igarashi Takamichi , Araki Kenichiro , Harimoto Norifumi , Tokunaga Fuminori , Shirabe Ken 
Published: January 2024

AbstractRNF31 is a multifunctional RING finger protein implicated in various inflammatory diseases and cancers. It functions as a core component of the linear ubiquitin chain assembly complex (LUBAC), which activates the nuclear factor kappaB (NF-κB) pathway via the generation of the Met1-linked linear ubiquitin chain. We aimed to clarify the role of RNF31 in the pathogenesis of hepatocellular carcinoma (HCC) and its relevance as a therapeutic target. High RNF31 expression in HCC, assessed by both immunohistochemistry and mRNA levels, was related to worse survival rates among patients with HCC. In vitro experiments showed that RNF31 knockdown in HCC cell lines led to decreased cell proliferation and invasion, as well as suppression of tumor necrosis factor (TNF)-α-induced NF-κB activation. Treatment with HOIPIN-8, a specific LUBAC inhibitor that suppresses RNF31 ubiquitin ligase (E3) activity, showed similar effects, resulting in decreased cell proliferation and invasion. Our clinical and in vitro data showed that RNF31 is a prognostic factor for HCC that promotes tumor aggressiveness via NF-κB activation.