bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological analysis and preventive strategies of pediatric burns inpatients in a Hangzhou hospital from 2020 to 2022: a retrospective study
Authors: Zhou Yichong , Dai Yefeng , Yue Xiaojie , Liu Yuxin , Zhao Xiong , Lin Li 
Published: December 2023

Abstract
Objective
The epidemiological characteristics of pediatric burn inpatients in our hospital over the past three years were analyzed to provide some advice for prevention and treatment strategies.
Methods
Data from 728 pediatric burn inpatients younger than 14 years of age admitted to our unit between January 1, 2019, and December 31, 2019, were collected. Age, gender, injury condition, injury factors, injury time, postinjury treatment and prognosis were analyzed.
Results
The proportion of children in the 1–3 age group was the highest (457 patients, 62.8%). 161 burn injuries (22.1%) occurred at a single site, and 567 burn injuries (77.9%) occurred at two or more sites at the same time. The burn sites from the most to least common were the trunk, upper limb, lower limb, head, face and neck, hip and perineum. The burn area was mostly between 5%-15% of the total body surface area (TBSA) (433 patients, 59.5%), the burn depth was mostly shallow (Ⅱ° to deep Ⅱ°) (405 patients, 55.6%), and the severity was mostly moderate (470 patients, 64.6%). Hydrothermal scald (673 patients, 92.4%) was the main injury factor in this group, followed by flame burn (32 patients, 4.4%). With increasing age, the proportion of hydrothermal scald gradually decreased, and the proportion of flame and other causes of burns gradually increased; these differences were statistically significant (х2= 47.471, P < 0.01). There were 672 burn cases in the household (92.3%) and 56 burn cases in outdoor and public places (7.7%). The proportion of outdoor and public burn injuries in rural children was greater than that in urban and suburban children. The daily peak burn times were 11:00–13:00, 17:00 and 20:00, and the monthly peak burn times were May, July and October, respectively. There were only 183 patients (25.1%) who received regular cold therapy after injury. Clinical outcomes were significantly different between patients who received reasonable treatment and those who did not (Z = 3.439, P < 0.01) and between those who received rational treatment and those who received irrational treatment (Z = 2.040, P < 0.05). Among the pediatric burn inpatients discharged with complete healing of the II° wound, the hospital stay of the children covered with acellular dermis was significantly shorter than that of the children in the conventional dressing change group (t = 3.646, P = 0.004).
Conclusion
Pediatric burns often occurred at 1–3 years of age, and most of the children had Ⅱ° combined deep burns. Indoor hydrothermal scald was the main injury factor, and regular cold therapy was not widely used. Early application of acellular dermis covering II° wounds can accelerate wound healing and reduce the length of hospital stay.
Increased serum caspase-1 in adult-onset Still’s disease
Authors: Matsumoto Haruki , Yoshida Shuhei , Koga Tomohiro , Fujita Yuya , Sumichika Yuya , Saito Kenji , Sato Shuzo , Asano Tomoyuki , Mizokami Masashi , Sugiyama Masaya , Migita Kiyoshi 
Published: December 2023

Abstract

Background
Caspase-1 is an essential component in the inflammasome activation cascade. This study assessed the potential of serum caspase-1 level as an inflammatory biomarker in patients with adult-onset Still’s disease (AOSD).
Methods
The study included 51 consecutive patients diagnosed with AOSD based on the Yamaguchi criteria, 66 patients with rheumatoid arthritis (RA) as a disease control, and 36 healthy subjects. Serum concentrations of caspase-1 were measured using enzyme linked immunosorbent assay (ELISA). In AOSD patients, the serum levels of 69 cytokines were analyzed using a multi-suspension cytokine array, and a cluster analysis of each cytokine was performed to identify specific molecular networks.
Results
AOSD patients had significantly increased serum caspase-1 levels versus RA patients (p < 0.001) and healthy subjects (p < 0.001). Serum caspase-1 also had significant positive correlations with AOSD disease activity score (Pouchot score r = 0.59, p < 0.001) and serum ferritin (r = 0.54, p < 0.001). Furthermore, among AOSD patients, significant correlations existed between serum caspase-1 and inflammatory cytokines, including IL-18. On immunoblot analysis, the cleaved form of caspase-1 (p20) was detected in the serum of untreated AOSD patients not in those from inactive AOSD patients receiving immunosuppressive treatments.
Conclusions
Caspase-1 can be a useful biomarker for the diagnosis and monitoring of AOSD. Activation of caspase-1 could be correlated with the inflammatory component of AOSD, specifically through the induction of proinflammatory cytokines via inflammasome activation cascades.
Temperature Dependence of Viscoelasticity of Lubricating Oil with Adsorptive Polymer Additives Sheared in Nanogaps
Authors: Nozue Takumi , Itoh Shintaro , Okubo Naoya , Fukuzawa Kenji , Zhang Hedong , Azuma Naoki 
Published: December 2023

Abstract
Adsorptive polymer additives have been reported to improve the retention capacity of oil films under hydrodynamic lubrication and to reduce friction under boundary lubrication. These effects are believed to result from the formation of a polymer adsorption film on the surface that acts as a lubricious coating. Polymer adsorption films have become dominant in nanometer-order microscopic gaps. However, their mechanical properties are difficult to quantify. This hinders the development of polymer additives. In our previous study, we successfully measured the shear viscoelasticity of lubricants (base oils) sheared in nanogaps using an originally developed measurement method called the fiber wobbling method (FWM). In this study, we measured the shear viscoelasticity of polymer-added lubricants in nanogaps by using FWM. In addition, we developed a heating stage in the FWM to quantify the temperature dependence of shear viscoelasticity in nanogaps. As a result, the viscosity index improved and elasticity was observed in the nanogap, where the polymer adsorption film was dominant. Furthermore, our results indicate that the elasticity of the adsorbed polymer film originates from entropic elasticity.
Cultural Characteristics, Genetic Diversity and Population Structure Analysis of an Emerging False Smut Pathogen Ustilaginoidea Virens Collected from Different Agro Climatic Zones of India
Authors: Bashyal Bishnu Maya , Yadav Gaurav Kumar , Chugh Chetna , Sharma Sapna , Sunani Sunil Kumar , Shakouka Mohamad Ayham , Gupta Ashish Kumar , Krishnan Gopala 
Published: December 2023

Abstract

Rice false smut (RFS) instigated by the fungus Ustilaginoidea virens, has emerged as one of the most devastating grain diseases in the mainstream of rice-planting regions worldwide. A total of fifty six distinct isolates of Ustilaginoidea virens collected from different states of India were characterized morphologically and also at molecular level. Isolates showed variation in the size of the spore, color of mycelium and shape of the hyphae. The spores were globose, irregularly round to elliptical and warty on the surface with diameters ranging from 4.12 to 6.34 µm. The maximum colony diameter was found in Telangana isolate Uv305 (66.00 mm) and minimum was in Uttar Pradesh isolate Uv214 (17.50 mm). Genetic Diversity was analyzed by using 12 SSR primers out of which 7 were found to be polymorphic The number of alleles per locus vary from 5 to10. The maximum number of polymorphism was shown by UvSSR97 followed by UVSSR79, UvSSR226 and Uv SSR276 while least polymorphism was observed in UvSSR107, and Uv163 primer. The Dendrogram generated based on polymorphic data revealed a considerable amount of diversity by grouping the isolates in five clusters. The PIC ranged from 0.079 to 0.900. The value of Shannon information index ranged from 2.99 to 4.52. The pairwise population fixation index (FST) value also indicated significant genetic variation among all compared geographical populations. All the isolates could be grouped into three groups according to their genetic structure confirmed through model based cluster algorithm using STRUCTURE v2.3.4. This in-depth understanding of U. virens population structure and diversity holds valuable implications for effectively managing rice false smut disease on a comprehensive scale.
Yu Linzhu alleviates primary ovarian insufficiency by improving proliferation and energy metabolism of granulosa cells through HIF1α/Cx43 pathway
Authors: Ruan Xin , Wang Pengxu , Wei Maolin , Yang Qingqing , Dong Xiaoying 
Published: December 2023

Abstract
Background
Yu Linzhu (YLZ) is a classical Chinese traditional fomula, which has been used more than 600 years regulating menstruation to help pregnancy. However, the mechanism of modern scientific action of YLZ needs to be further studied.
Methods
Thirty SD female rats were divided into three groups to prepare the blank serum and drug-containing serum, and then using UHPLC-QE-MS to identify the ingredients of YLZ and it’s drug-containing serum. Twenty-four SD female rats were divided four groups, except the control group, 4-vinylcyclohexene dicycloxide (VCD) was intraperitoneally injected to establish a primary ovarian insufficiency (POI) model of all groups. By using vaginal smear to show that the estrous cycle of rats was disturbed after modeling, indicate that the POI model was successfully established. The ELISA test was used to measure the follicle-stimulating hormone (FSH), estradiol (E2) and anti-Mullerian hormone (AMH) levels in the serum of rats. HE stain was used to assess the morphology of ovarian tissue. The localization and relative expression levels of CX43 protein were detected by tissue immunofluorescence. Primary ovarian granulosa cells (GCs) were identified by cellular immunofluorescence. CCK8 was used to screen time and concentration of drug-containing serum and evaluate the proliferation effect of YLZ on VCD-induced GCs. ATP kit and Seahorse XFe24 were used to detect energy production and real-time glycolytic metabolism rate of GCs. mRNA and protein expression levels of HIF1α, CX43, PEK, LDH, HK1 were detected by RT-PCR and WB.
Results
UHPLC-QE-MS found 1702 ingredients of YLZ and 80 constituents migrating to blood. YLZ reduced the FSH while increased the AMH and E2 levels. In ovarian tissues, YLZ improved ovarian morphology, follicle development and the relative expression of CX43. In vitro studies, we found that YLZ increased the proliferative activity of GCs, ATP levels, glycolytic metabolic rate, HIF1α, CX43, PEK, HK1, LDH mRNA and protein levels.
Conclusions
The study indicated that YLZ increased the proliferation and glycolytic energy metabolism of GCs to improve the follicular development so that further alleviated the ovarian function.
Association Between Early Echocardiography Screening and Intraventricular Hemorrhage in Preterm Infants: A Prospective Observational Multicenter Study
Authors: Terroba-Seara Sandra , Oulego-Erroz Ignacio , Palanca-Arias Daniel , Galve-Pradel Zenaida , Delgado-Nicolas Sara , Pérez-Pérez Alicia , Rodríguez-Ozcoidi Jorge , Lavilla-Oiz Ana , Bravo María Carmen , Lavanda-Montalvo Leticia , Méndez-Abad Paula , Zafra-Rodríguez Pamela , Rodeño-Fernández Lorena , Montero-Gato Jon , Bustamante-Hervás Carmen , Val Cristina Vega-Del , Rodríguez-Fanjul Javier , Mayordomo-Colunga Juan , Alegría-Echauri Iosune , Pérez-González Andrea 
Published: December 2023

Abstract
Purpose:To determine whether the application of a protocolized early echocardiographic screening for low systemic blood flow and guided inotropic therapy is associated with reduced intraventricular hemorrhage in preterm infants.
Methods: Prospective, observational multicenter study between September 2020 and September 2021 in nine neonatal intensive care units. Preterm infants below 33 weeks of gestational age were eligible. The exposure variable was early targeted neonatal echocardiography screening for low systemic blood flow. Five of the centers performed early screening and guided inotrope therapy (intervention group), and 4 centers did not (control group). Our main outcome was >grade II intraventricular hemorrhage or death within the first 7 days of life. Inverse probability of treatment weighting based on the propensity score accounting for the clustered nature of data was used for the main analysis.
Results: 332 preterm infants (131 in the intervention group and 201 in thecontrol group) were included. Protocolized early echocardiography screening for low systemic blood flow and guided inotropic treatment was associated with a significant reduction in > grade II intraventricular hemorrhage or early death (odds ratio 0.285 (95% CI: 0.133-0.611); p=0.001)
Conclusions: Early echocardiography screening for low systemic blood flow and guided inotropic treatment may reduce the incidence of intraventricular hemorrhage in preterm infants.
Clinical Features at Diagnosis Are Associated With the Need of Emergency Invasive Procedures and Intensive Care Unit Admission in Patients With High-risk Neuroblastoma – A Retrospective Cohort Study
Authors: Voglino Valerio , Persano Giorgio , Martucci Cristina , Ioris Maria Antonietta , Castellano Aurora , Serra Annalisa , Prete Laura Del , Giordano Ugo , Natali Gian Luigi , Paolo Pier Luigi , Stracuzzi Alessandra , Crocoli Alessandro , Inserra Alessandro 
Published: December 2023

Abstract
Purpose
Patients affected by high-risk may occasionally need emergency procedures due to acute complications during induction chemotherapy.
The aim of the present study is to find risk factors associated with these complications.
Methods
Patients diagnosed with high-risk neuroblastoma from January 2013 until February 2022 were retrospectively reviewed.
Primary outcomes were the need of emergency invasive procedures and the Intensive Care Unit (ICU) admission.
Clinical data at diagnosis were recorded and the correlation with the primary outcomes was analyzed.
Results
Fifty patients were diagnosed with high-risk neuroblastoma.
Six patients required emergency procedures for hemothorax (2 patients), hemothorax and hemoperitoneum (1 patient), hydronephrosis (3 patients); two of them were admitted to ICU.
Factors associated with emergency procedures were stage 2 hypertension (p = 0.0001), higher number of vascular IDRF (p= 0,0068), larger diameter of the primary tumor (p = 0,0043), high LDH levels (p < 0.0001) and low levels of urinary VMA (p < 0.0001).
Factors associated with ICU admission were stage 2 hypertension (p = 0,0229), high levels of LDH (p < 0.0001) and low levels of urinary VMA (p< 0.0001).
Discussion
Specific features at diagnosis are associated with the need of emergency invasive procedures and ICU admission in high-risk neuroblastoma patients.
Numerical simulation of contact surface stress distribution based on stress-magnetization effect surface
Authors: Zhao Shuai , Xu Xinjun , BI Yaxuan 
Published: December 2023

Abstract

Taking the rough surface of C45 steel grinding as the research object, we established a two-dimensional finite element model of the rough surface and its surrounding air by using ANSYS, and studied the contact properties between the rough surface and the rigid plane under normal load (≤ 10MPa). On this basis, ANSYS Apdl language was used to establish a force-magnetic coupling model, and further simulated the impact of different load conditions on the leakage magnetic field on the contact surface. The results showed that according to the zero-crossing point of the normal component of the leakage magnetic field or the extreme point of the tangential component, the number of stress concentrations on the contact surface and the stress level of the corresponding area can be effectively determined. This method has certain reference value for studying the surface contact stress distribution between parts.
Delineating the relationship between planting density and agronomic performance in hybrid winter wheat for elevated yield and nitrogen utilization in the eastern Huang-Huai-Hai Region
Authors: zhang yulu , Du yihang , Li wenlu , Zhu Zixin , Jia Jing , Meng Fanggang , Zhang Shenquan , zhao changxing 
Published: December 2023

Abstract

The study aimed to clarify the impact of different planting densities on accumulation and remobilization of dry matter and nitrogen as well as yield in hybrid winter wheat varieties and their relationships, providing the theoretical basis for high-yield cultivation of hybrid winter wheat in the eastern Huang-Huai-Hai region. During the 2021–2023 winter wheat growing seasons, hybrid variety ‘Jingmai 17’ and conventional variety ‘Jimai 22’ were selected as experimental materials. The study investigated the effects of three planting densities (150 plants/m², 300 plants/m², and 450 plants/m²) on the flag leaf SPAD value, leaf area of per culm (LAC), dry matter and nitrogen accumulation and remobilization, grain yield (GY), and its components during the winter wheat growth period. The findings indicated that the hybrid variety ‘Jingmai 17’ maintained a larger LAC after anthesis, exhibited a slower decline in flag leaf SPAD value during the middle to late grain-filling stages, and had a longer duration of green leaf area post-anthesis, which was conducive to the accumulation of photosynthetic assimilates. This was evidenced by the higher biomass at maturity and post-anthesis, as well as biomass remobilization, thousand-grain weight (TGW), and grain numbers per unit area (GN), compared to ‘Jimai 22’, displaying a distinct heterosis with a yield exceeding standard of up to 10.6%. At a planting density of 300 plants/m², both varieties achieved a suitable LAC, which helped to maintain flag leaf SPAD values during the grain-filling stages, and a slower rate of leaf senescence post-anthesis. This suitable population density enhanced the biomass at maturity, post-anthesis, and the harvest index (HI), resulting in higher GN and TGW, and thus, a higher yield. At 300 plants/m², both varieties not only had higher nitrogen accumulation at maturity but also maintained high pre-anthesis nitrogen remobilization and remobilization rate, and the higher yield resulted in moderate nitrogen use efficiency (NUE). Notably, ‘Jingmai 17’ exhibited significant heterosis in nitrogen harvest index (NHI) and NUE. In conclusion, the hybrid variety ‘Jingmai 17’ demonstrated significant heterosis, and a planting density of 300 plants/m² was found to optimally balance yield and NUE.
Myeloid signature in the tumor immune microenvironment impacts the prognosis of urachal carcinoma
Authors: Zhou Tailai , Chen Hengxin , Wang Yuhang , Wu Menghai , Wang Yinzhao , Dao Pinghong , Huang Chuyang , Li Yong , Yan Yuzhong , Chen Minfeng 
Published: December 2023

Abstract
Purpose
Urachal carcinoma, an infrequent yet aggressive tumor, lacks extensive research regarding its pathogenesis and prognosis. The tumor immune microenvironment (TIME) significantly influences the prognosis of malignant tumors. Consequently, understanding the role of TIME in urachal carcinoma remains a critical and unmet need.
Methods
We assessed TIME markers in both tumoral (T) and stromal (S) regions of urachal carcinoma using immunohistochemistry. Subsequently, a risk prediction function (Riskscore) was developed through Lasso regression analysis. The performance of Riskscore was validated in different cohorts using various statistical analyses. Additionally, pathway enrichment analysis was employed to unveil potential mechanisms underlying tumor progression, leading to the identification of potential therapeutic drugs.
Results
Among the 15 cell markers examined, CD16S, CD68S, and CD11bS exhibited significant associations with overall survival in urachal carcinoma. The Riskscore, constructed based on these three myeloid markers, accurately predicted prognosis, offering insights into potential pathways influencing urachal carcinoma progression and identifying promising candidate drugs.
Conclusions
In this study, we established a Riskscore based on the myeloid signature of urachal carcinoma and, for the first time, conducted genetic sequencing for urachal carcinoma. This elucidated insights into the immune landscape and potential therapeutic agents. Our work contributes to advancing the diagnosis and treatment strategies for urachal carcinoma.
Complex biological network analysis revealed the regulation of genes under multiple fungal infected silk of Zea mays
Authors: Kumar Amrendra , Arunachalam Annamalai , Lakshmi PTV 
Published: December 2023

Abstract
Background
Maize (Zea mays L.) is a more versatile and multi-purpose crop, primarily grown as food and feed, but now extensively applied in beauty, drug, and biofuel energy. However, the decline in production is reported to be promoted by the fungal pathogens, which invade developing grain using silk channels and hampering the quantity and quality of maize silk. Since understanding the molecular mechanisms will increase the chance of reducing the fungal infection, an integrated omics approach was undertaken on the transcriptome datasets with the multi-fungus at the maize silks.
Results
The available data sets were classed to A and B for simplicity, but enhancement in the analysis based on the intra and inter-transcriptomic level, keeping control data sets for comparing under the infected conditions. Hence, based on the analysis, the differentially expressed transcript isoforms were identified, which revealed approximately 39651 and 34027 transcript isoforms, 3819 and 3320 alternatively spliced regions along classes A and B. On examination, about 3212 lncRNAs were identified, among which 530 lncRNAs expressed were recognized to play a key role in multiple infectious conditions. Investigation on the complex biological network mediated by the combinations of miRNAs with lncRNAs, lncRNAs with transcript isoforms, lncRNAs with alternatively spliced genes. Interestingly, about 82 lncRNAs exhibited intersectional interactions with miRNAs, AS, and Tiso, which decoded for 103 genes.
Conclusions
This study might have contributed to key functions of response to stimulus, regulating the metabolic and cellular process during the development and of the plants, which eventually enhanced the plants immune or defence mechanisms.
Kirillov-Lipsman orbit method of a class of Gelfand pairs
Authors: Rahali Aymen 
Published: December 2023

Abstract
Let $G:=K\ltimes N$ be the semidirect product with Lie algebra $\mathfrak{g},$ where $N$ is a connected and simply connected nilpotent Lie group, and $K$ is a subgroup of the automorphism group, $Aut(N),$ of $N.$ We say that the pair $(K,N)$ is a nilpotent Gelfand pair when the set $L_K^1(N)$ of integrable $K$-invariant functions on $N$ forms an abelian algebra under convolution.According to Lipsman, the unitary dual $\widehat{G}$ of $G$ is in one-to-one correspondence with the space of admissible coadjoint orbits $\mathfrak{g}^\ddag/G$ of $G.$ Under some assumptions on the pair $(K,N)$ we will show in this work, that the quotient topology of $\mathfrak{g}^\ddag/G$ could be read from the usual topology of some parameters space $\mathcal{P},$ called Mackey's parameters space (will be defined below in section 3). Furthermore, we show that the Kirillov-Lipsman bijection $$\widehat{G}\simeq\mathfrak{g}^\ddagger/G$$is a homeomorphism for a class of Lie groups associated with the nilpotent Gelfand pairs $(K,N).$