bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative computed tomography guided coil versus suture hook-wire localization for multiple pulmonary nodules
Authors: Dou Yue , Song Hai-Hong , Shi Yi-Bing , Gao Yong-Guang , Bai Sheng-Jie 
Published: January 2025

Abstract

Background
Computed tomography (CT)-guided coil and suture hook-wire (SHW) insertion are widely employed for the preoperative localization of multiple pulmonary nodules (PNs). However, the comparative effectiveness and safety of these two techniques remain unclear. This study aimed to probe the clinical efficacy and safety of coil and SHW-based preoperative localization in patients with multiple PNs.
Methods
Patients with multiple PNs who underwent CT-guided coil or SHW localization prior to video-assisted thoracic surgery (VATS) resection between January 2020 and December 2024 were enrolled retrospectively. Localization outcomes and VATS-related parameters were compared.
Results
A total of 35 patients (76 PNs) in the coil group and 37 patients (81 PNs) in the SHW group were included. Technical success rates for localization were 100% in both groups. However, the mean duration of CT-guided localization was significantly shorter in the SHW group than in the coil group (24.0 ± 12.3 minutes vs. 29.3 ± 9.0 minutes, P = 0.042). Pneumothorax incidence rates were comparable between the coil and SHW groups (25.7% vs. 29.7%, P = 0.704). Both groups achieved 100% technical success rates for VATS limited resection, and all patients underwent successful one-stage multiple limited resections. The mean VATS duration (127.0 ± 42.2 minutes vs. 106.4 ± 66.3 minutes, P = 0.122) and median blood loss (25 ml vs. 50 ml, P = 0.152) were also similar in both groups.
Conclusions
Based on these results, both CT-guided coil and SHW localization strategies are safe and effective methods for the preoperative localization of multiple PNs. However, SHW localization offers a significant advantage in reducing procedure time compared to coil localization.
Pengelolaan Stakeholder dalam Pendidikan Islam
Authors: Muhamad Sidik Asep Hilmi , Mulyawan Mulyawan 
Published: January 2025

Abstract: Pendidikan merupakan sesuatu yang paling penting untuk mencetak generasi yang akan datang dalam kemajuan negara melalui keilmuan yang dihasilkan. Manusia dalam menjalani kehidupan tidak bisa dilepaskan dengan lingkungan Pendidikan. Pendidikan tersebut kaitan erat dengan lingkungan keluarga, seoklah dan Masyarakat. Dimana ketiga komonen tersebut sangat berkaitan erat dalam mencetak dan mewujudkan Pendidikan yang sehat dan berkualitas. Dalam Pendidikan tentunya memiliki proses kehidupan yang Panjang dan kompleks. Manajemen Pendidikan tentunya berhubungan erat dengan stakeholders Pendidikan untuk meningkatkan sumber daya manusia yang memiliki kualitas dan bermutu tinggi. Tujuan dari pembahasan ini tentunya untuk mengetahui stakeholders dalam Pendidikan islam di dalam realitas Masyarakat muslim dewasa kini, menegetahui perkembangan Masyarakat globa, mengetahui kompetensi lulusan Pendidikan islam yang dibutuhkan dan mengetahui rancangan kompetensi lulusan. Kata kunci: : Stakeholders, Pendidikan dan Islam
The Role of Sirtuins in Diabetic Nephropathy: A Comprehensive Review
Authors: Wal Pranay , Tarannum Tarannum , Baldaniya Lalji , Kaur Kiranjeet , Singh Priyanka , Aziz Namra , Singh Komal , Gasmi Amin 
Published: January 2025

Introduction:
Diabetic nephropathy is characterized by elevated oxidative stress and
chronic inflammation in the kidneys. A class of proteins called sirtuins is well-known to be important
for a number of cellular functions, such as metabolism, stress tolerance, and ageing. Among
them, SIRT1 is associated with the progression of diabetic nephropathy, a dangerous kidney-related
consequence of diabetes mellitus. Thus, this study aims to examine the function and pathways
of sirtuin that are responsible for the progression of this disease.

Methods:
Publications considered from the standard databases like PUBMED-MEDLINE, Google
Scholar, and Scopus using standard keywords, “Sirtuin,” Signalling pathway”, and “Diabetic
Nephropathy” well described the actual knowledge on the scientific literature indicating patient
susceptibility to kidney disease that is influenced by sirtuin-1 gene variants.

Results:
The research results imply that sirtuins offer enormous promise as cutting-edge therapeutic
targets for kidney disease prevention and management. Renal fibrosis, metabolic disorders, renal
impairment, and a possible regulation mechanism all probably entail blocking inflammation
through various signalling pathways.

Conclusion:
A comprehensive understanding of the fundamental pathophysiological pathways targeting
sirtuin is essential as a diagnostic tool. For the treatment of diabetic nephropathy, researchers
are developing therapeutic techniques to target biological roles and functions of different types
of sirtuin, processes, and signalling pathways.
Targeting Glutamine Metabolic Reprogramming in Pancreatic Cancer: Current Insights and Future Directions
Authors: Ma Yanhui , Wang Mingling , Zhang Kexing , Ren Fangbing , Wang Yuqun , Zhang Wenqiang , Kan Chengxia , Han Fang , Sun Hongxi , Sun Xiaodong 
Published: January 2025

Abstract:
Pancreatic Cancer (PC) is a devastating malignancy with a poor prognosis and in-creasing morbidity. Current treatment strategies have limited efficacy in improving patient survival. Metabolic reprogramming is a hallmark of cancer and plays a key role in the pro-gression and maintenance of PC. PC cells exhibit a unique glutamine metabolism that is dis-tinct from other cancer types. The non-classical pathway of glutamine metabolic reprogram-ming plays a “permissive role” in the survival and proliferation of PC cells, mainly by affect-ing the redox homeostasis of the cells. In this review, we compare and contrast the canonical and non-canonical glutamine metabolic pathways and highlight recent advances in targeting non-canonical glutamine metabolism for therapeutic intervention. This may provide novel in-sights and opportunities for exploiting glutamine metabolic reprogramming in PC treatment.
Macranthoside B Suppresses the Growth of Adenocarcinoma of Esophagogastric Junction by Regulating Iron Homeostasis and Ferroptosis through NRF2 Inhibition
Authors: Wang Lingling , Pan Guangzhao , Tian Sichao , Zhang Che , Tao Fangfang , Qin Jiang-Jiang 
Published: January 2025

Background:
Macranthoside B (MB) is a saponin compound extracted from hon-eysuckle that has been reported to exhibit significant medicinal values, particularly anti-tumor activities. This study aimed to evaluate the anticancer efficacy of MB in treating adenocarci-noma of the esophagogastric junction (AEG) and elucidate its underlying mechanisms.

Methods:
Three AEG cell lines and normal gastric epithelial cells were used to assess the an-ticancer activity of MB in vitro. A series of experiments, including RNA sequencing (RNA-seq) analysis, transmission electron microscopy (TEM), immunofluorescence, and western blot assay, were conducted to validate the molecular mechanisms by which MB may mediate these physiological changes. Finally, we used shRNA assays to silence the key gene driving these changes and examined the expression of molecules involved in the affected pathways.

Results:
MB exhibited significant anti-AEG cell activity with IC50 values ranging from 9.5 to 12.7 μM. RNA-seq results indicated that MB treatment in AEG cells significantly altered mRNA levels of autophagy- and ferroptosis-related genes. Further experiments revealed that MB treatment led to the up-regulation of lipid reactive oxygen species (Lip-ROS), oxidative stress-related pathway genes, and LC3B-labeled autophagic vesicles in AEG cells. Moreover, MB mediated NCOA4-dependent ferritinophagy, disrupting iron homeostasis and causing subsequent ferroptosis. We further confirmed that the intrinsic connection between autophagy and ferroptosis was due to the inhibition of NRF2 by MB. The inhibition of NRF2 by MB triggered transcriptional repression of its downstream effector molecules HERC2 and VAMP8, thus stabilizing NCOA4.

Conclusion:
This study demonstrated MB to inhibit AEG cell growth by regulating iron ho-meostasis and inducing ferroptosis through the inhibition of NRF2, providing a basis for the development of novel drugs for AEG treatment
SRT1720 Treatments Hepatic Ischemia Reperfusion Injury by Regulation of NF-κB Signaling Pathways and Reduce Cell Apoptosis: From Network
Pharmacology to Experimental Validation
Authors: Li Zhongzhe , Geng Wenting , Yu Beilei , Wang Bin , Sun Shuxuan , Zhou Lu 
Published: January 2025

Background and objective:
Hepatic ischemia reperfusion injury (HIRI) is a common
complication closely related to the prognosis of liver surgery, and effective treatment methods
are still unavailable. SRT1720 has the characteristics of multifunction and multitarget which may
cope with the multidirectional complex pathological process caused by HIRI. The present study
aimed to explore the potential mechanism of SRT1720 in HIRI through a combination of network
pharmacology, in vitro experiments and in vivo models.

Methods:
Differentially expressed genes (DEGs) were identified based on the GSE15480 and
Genecards database. Enrichment analyses were then conducted. SRT1720-targeted genes were
obtained through databases such as Chembl, TTD, GtoPdb, and so on. All target genes were
standardized by the Uniprot database and the Kyoto Encyclopedia of Genes and Genomes
(KEGG) pathways were identified by STRING. Shared KEGG pathways were identified using a
Venn diagram among SRT1720-targeted pathways and HIRI. Furthermore, experimental techniques
such as cell apoptosis assay and western blotting were used to confirm the most significant
biological processes and the key pathway between SRT1720-targeted and HIRI.

Results:
This study identified 118 HIRI-related DEGs, 69 shared KEGG pathways of SRT1720
and HIRI. In addition, the findings revealed that SRT1720 significantly reduced liver ischemiareperfusion
(I/R) injury. NF-κB signaling pathway and the expression of promoting apoptosis
factors such as Bax and Caspase3 were inhibited, while antiapoptotic protein Bcl-2 was promoted
in the SRT1720 group compared with the I/R group.

Conclusion:
The findings indicate that SRT1720 may inhibit the development of HIRI by inhibiting
the NF-κB signaling pathway and reducing cell apoptosis, acting as a treatment for HIRI.
First Report of HPV Genotyping and Distribution in People Living with
and Without HIV from Iran and the Middle East
Authors: Maleki Faezeh , Farahmand Mohammad , Keyvani Hossein 
Published: January 2025

Aims:
In people living with human immune deficiency (PLHIV), the rates of human papillomavirus
(HPV) infection, mixed types, and high-risk (HR) strains increase, while the virus
clearance is prevented. Here, we report HPV genotyping in PLHIVs from Iran and the Middle
East region for the first time.

background:
In people living with human immune deficiency (PLHIV), the rates of human papillomavirus (HPV) infection, mixed types, and high-risk (HR) strains increase, while the virus clearance would be called off.

Methods:
HPV genotyping in referring individuals from different provinces to our laboratory was
evaluated over 2023-2024. For this, the HPV types in specimens were detected through the INNO-
LiPA HPV genotyping kit. Statistical analysis was conducted with a 95% confidence interval
(95%CI) and P < 0.05. Accordingly, 481 subjects from various provinces participated in this
study.

Results:
The rate of HPV infection was 45.7%, of which 14% were HIV-infected women referred
from all provinces. The most prevalent types included 6, 51, and 18, but not 16 HR types. Mixed
infections in dually infected women were significantly more than in HPV-infected ones. The
HPV+/HIV+ subgroup had the lowest median age. The prevalence of HPV types and mixed infection
in PLHIVs was congruent with the previous reports, except for the low rate of type 16 infection,
perhaps due to the healthy nature of our subjects. Only HIV+/HPV+ cases' age was similar
to the previous reports, perhaps because of sample collection and study designs. Among all factors,
age and gender affected the HPV type distribution notably.

Conclusion:
The current study corroborated the results of many prior reports, demonstrating the
considerable impact of HIV status on HPV distribution. The authors recommend implementing a
national HPV vaccination and more comprehensive reports of HPV genotyping in PLHIVs.

other:
NA
Efficacy of Ozonated Olive Oil as a Topical Treatment for Dermatophytosis Caused by T. mentagrophytes in Rat Model
Authors: Mohaghegh Ali , Mansouri Mahdi , Etemad Leila , Soheili Vahid , Haghighzadeh Atoosa , Dadpour Saba , Rajabi Omid 
Published: January 2025

Introduction:
Dermatophytosis is a significant global health issue that poses a serious risk
to public well-being. This fungal infection occurs when dermatophytes invade the outer surfaces of
both human and animal bodies. The use of common anti-dermatophyte medications has some limitations
due to their side effects and drug resistance. Herbal remedies offer broad-spectrum antimicrobial
effects low toxicity, and are widely used compared to traditional chemical fungicides. Recent studies
have indicated that the ozone has antifungal activity due to its high oxidizing power and skin
penetrating capacity.

Methods:
This study examined the antifungal effects of ozonated olive oil in treating dermatophytosis
caused by Trichophyton mentagrophytes through in vitro and in vivo tests on rats. The agar dilution
method was used to establish the treatments' minimum inhibitory concentrations (MICs). For the
in vivo study, thirty male Wistar albino rats from the Laboratory Animal Unit were prepared. The
topical antifungals were then applied topically once daily for 7 days to the animals beginning 10 days
after the appearance of symptoms. Five groups of animals were treated with virgin olive oil, ozonated
olive oil (1.25, 2.5, or 5 mg/mL), and terbinafine (1.25 g/mL).

Results:
Following the therapy period, clinical and mycological efficacies were assessed. Ozonated
olive oil and terbinafine were shown to have MIC of 2.5 μg/mL and 0.62 μg/mL against T. mentagrophytes,
respectively. Ozonated olive oil showed antifungal effects and significantly reduced the
clinical score by increasing hair growth and decreasing inflammation, similar to terbinafine.

Conclusion:
Therefore, ozonated olive oil may serve as an alternative treatment for Trichophyton
infections.
Phillygenin: A Versatile Multi-Targeted Anti-inflammatory, Hepatoprotactive
and Anticancer Agent
Authors: Tailor Navin Kumar 
Published: January 2025

Background:
Multi-target drugs are gaining attention for treating chronic diseases. Phillygenin (C21H24O6), a compound in Forsythiae Fructus, demonstrates strong anti-inflammatory, antifibrotic, anticancer, antioxidant, and antimicrobial properties. Its polar functional groups allow for
structural modifications, offering the potential for novel drug discovery and development.

Objective:
This review provides an overview of Phillygenin as a scaffold for multi-target drug development against chronic disease and disorders. It examines the molecular mechanisms behind its therapeutic effects, focusing on targets like NF-κB, PI3K/AKT, MAPK, Nrf2-ARE, P2X7R/NLRP3,
Ca2+-calcineurin-TFEB, JAK/STAT, Notch1, TGF-β/Smads, and AMPK/ERK/NF-κB.

Methods:
A systematic review of the literature on Phillygenin was conducted to explore its therapeutic applications. The study examined Phillygenin's molecular mechanisms and potential targets to
highlight its multi-targeting capabilities.

Results:
Phillygenin shows promise in managing chronic diseases by targeting multiple cellular
pathways, including NF-κB, PI3K/AKT, MAPK, Nrf2-ARE, P2X7R/NLRP3, Ca2+-calcineurinTFEB, JAK/STAT, Notch1, TGF-β/Smads, and AMPK/ERK/NF-κB. Its versatility and natural
origin make it a valuable scaffold for developing multi-target therapeutics.

Conclusion:
Exploring Phillygenin as a framework for multi-target drugs offers the potential to
boost efficacy and reduce side effects. Further research and clinical trials are needed to confirm Phillygenin's therapeutic potential.
Aberrant Expressions of EDNRB and EDN3 in a Multifactorial Hirschsprung Disease
Authors: Gunadi . , Vujira Khanza Adzkia , Amadeus Verrell Christopher , Gabriela Gita Christy , Amaragati Adisrasti Rejeki , Geometri Esensi Tarian , Eryvinka Laudria Stella , Ferdinandus Kevin Eliezer , Pramono Nabila Ardia , Dwihantoro Andi 
Published: January 2025

Background:
Hirschsprung disease (HSCR) is a multifactorial disorder due to the enteric
nervous system (ENS) development failure. At least 35 genes have been responsible for HSCR,
including EDNRB and EDN3. Here, we aimed to determine the EDRNB and EDN3 expressions effects
in HSCR subjects.

Methods:
Our study analyzed EDNRB and EDN3 expressions in the colon of HSCR subjects and
controls by a quantitative PCR. The EDNRB and EDN3 expressions were analyzed by the Livak
method (2-ΔΔC
T).

Results:
Twenty-seven HSCR patients and 20 controls were ascertained. EDNRB and EDN3 expressions
downregulated was found in ganglionic and aganglionic HSCR than control colons (EDNRB:
ΔCT 6.78 ± 1.38 vs. 1.71 ± 2.79; p = 0.0001 (ganglionic); ΔCT 4.41 ± 1.63 vs. 1.71 ± 2.79; p
= 0.0005 (aganglionic); and EDN3: ΔCT 7.60 ± 1.93 vs. 1.81 ± 2.89; p = 0.0001 (ganglionic); ΔCT
9.72 ± 4.32 vs. 1.81 ± 2.89; p = 0.0001 (aganglionic)). A significant difference in EDNRB and
EDN3 expressions was also noted between the HSCR colon: ganglionic vs. aganglionic segment
(p = 0.00002 and 0.017).

Conclusion:
We report the downregulated EDNRB and EDN3 expressions in HSCR subjects, indicating
EDNRB/EDN3 expressions have a significant responsibility in HSCR pathogenesis. Nevertheless,
we further clarify the complexity of the development of ENS.
Optimized Rutin-incorporating PEGylated Nanoliposomes as a Model wit Remarkable Selectivity Against PANK1 and MCF7 Cell Lines
Authors: Al-Samydai Ali , Al Qaraleh Moath , Al-Halaseh Lidia K. , Abu Hajleh Maha N , Carradori Simone , Abdulmaged Maryam , Kareem Rand , Alzaidi Hasanain , AK Mousa Mohamad , Al-Hiari Yusuf , Nsairat Hamdi , Alshaer Walhan 
Published: January 2025

Background:
This study aims to enhance the delivery of polyphenols using nanotechnology.

Objective:
To develop and evaluate liposomal formulations for improved delivery and stability of polyphenols,
specifically focusing on Rutin.

Methods:
Liposomal formulations were meticulously prepared via the Thin-Film Hydration method. Comprehensive
physical characterization was conducted, including stability assessments using Dynamic Light Scattering
(DLS) and Thermogravimetric Analysis (TGA). The free radical scavenging activity was measured using the
DPPH• assay, and MTT cell viability assays were performed to assess cytotoxicity.

Results:
The results demonstrated a significant reduction in nanoparticle size from 123 nm to 116 nm and an
increase in charge from -14 to -22 with rising Rutin concentrations. The formulation achieved enhanced homogeneity
at a Rutin concentration of 2.0 mg/mL and showed higher stability. Incorporating Rutin improved the formulation's
stability over 30 days, as evidenced by a decrease in the Differential Scanning Calorimetry peak temperature
from 58.65 °C to 54.42 °C. Rutin-loaded and co-loaded nanoliposomes exhibited remarkable selectivity
against PANK1 and MCF7 cell lines, with IC50 values of 2.13±0.35 μg/mL and 4.75±0.19 μg/mL, respectively.

Conclusion:
PEGylated Rutin-loaded nanoliposomes offer a promising platform for biodegradable and biocompatible
drug delivery systems, enhancing the bioavailability, solubility, and stability of the polyphenols.
Treatment of Primary Hypothyroidism by Slow-Release Liothyronine Monotherapy
Authors: Azizi Fereidoun , Amouzegar Atieh , Abdi Hengameh , Masoumi Safdar , Mehran Ladan 
Published: January 2025

Background:
Combination therapy with levothyroxine (L-T4) and slow-release T3 (SRT3)
in the treatment of hypothyroidism results in a normal triiodothyronine/thyroxine (T3/T4) ratio
above that of L-T4 monotherapy. No clinical study has been reported on SRT3 monotherapy
for hypothyroidism.

Methods:
This study was conducted in two parts. In the first part, 20 patients with primary hypothyroidism
and serum thyrotropin (TSH) >30 mU/L were randomized into three groups receiving
1.6 μg/kg L-T4, equivalent doses of SRT3 or L-T3 of 0.55 μg/kg for 4 weeks. Their fasting
serum-free T4 [fT4], T3, and TSH were measured weekly before taking medication for up to 4
weeks. In the second part, in 9 hypothyroid patients on L-T4 therapy and normal serum TSH, LT4
therapy was discontinued, and a once-daily dose of SRT3 of 0.55 μg/kg was replaced. Serum
fT4, T3, and TSH were measured weekly.

Results:
In part one, in patients treated with L-T3 and L-T4, serum TSH decreased to normal values
after 4 weeks of intervention. In 7 patients on SRT3, serum T3 increased from 47±12 at baseline
to 110±16 ng/dL, and serum TSH decreased from 60±11 at baseline to 24±10 and 26±7
mU/L, respectively, at 14 and 21 days after intervention. At the end of 28 days, mean serum T3
was 110±16, 168±74, and 96±18 ng/dL in SRT3, L-T3, and L-T4 groups, respectively (p <
0.001). In part two, serum fT4 decreased from 1.43±0.7 to 0.41±0.14 ng/dl, and serum T3 increased
from 86±21 to 113±27 ng/dL by 21 days. Mean serum TSH remained normal until 14
days but increased to 15.1±7.6 mU/L at 21 days. In the end, mean serum fT4, T3, and TSH were
0.35±0.17 ng/dl, 77.4±8.9 ng/dL, and 35±11 mU/L, respectively.

Conclusion:
In patients with primary hypothyroidism, SRT3 monotherapy with an equivalent
dose to L-T4 maintained normal serum T3 but could not sustain normal serum TSH concentration.