bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: Laparoscopy-assisted resection for intra-abdominal gossypiboma masquerading as a jejunal tumor (with video)
Authors: Han Yihui , Yang Wenming , Dai Wenshu , Ma Qin , Yuan Tao , Yang Yun , Lu Yanrong , Zhang Bo , Zhang Mingming 
Published: November 2023

IntroductionIntra-abdominal gossypiboma, a cotton-based retained foreign body after an abdominal surgery, is associated with various clinical manifestations and complications. Its infrequent occurrence and unpredictability make its early diagnosis particularly challenging. We herein present an atypical case of intra-abdominal gossypiboma mistaken for a jejunal tumor.Case presentationA 33-year-old female presented to the emergency room with an acute episode of progressive abdominal pain and distention, nausea, and vomiting for 20 hours. She had undergone an urgent cesarean section due to fetal tachycardia seven years prior. The initial diagnosis of small bowel obstruction (SBO) due to a jejunal tumor was established by computed tomography. Subsequent to successful medical management of the SBO, a laparoscopy-assisted resection of the mass and the adherent jejunal segment was conducted, culminating in a primary side-to-side jejunojejunostomy. Examination of the excised tissue revealed an approximately spherical fibrous mass, 6 × 6 × 5 cm in dimension, embedded in the jejunal wall, housing a 20 × 20-cm gauze. Postoperative recovery and routine follow-up ensued without complications.ConclusionIn light of this case, the need for clinicians to maintain an elevated awareness and suspicion of gossypiboma should be accentuated when evaluating an intra-abdominal mass, especially in patients with a prior history of high-risk laparotomy. Laparoscopic surgery stands out as a technically proficient and minimally invasive strategy for diagnosing and treating intra-abdominal gossypiboma. Besides, it is imperative to emphasize the importance of meticulous surgical procedures and postoperative protocols to prevent such oversights, reaffirming the need for consistent intraoperative counts and checks of surgical items.
Development and evaluation of RADA-PDGF2 self-assembling peptide hydrogel for enhanced skin wound healing
Authors: Deptuła M. , Sawicka J. , Sass P. , Sosnowski P. , Karpowicz P. , Zawrzykraj M. , Wardowska A. , Tymińska A. , Dzierżyńska M. , Pietralik-Molińska Z. , Peplińska B. , Zieliński J. , Kondej K. , Kozak M. , Sachadyn P. , Rodziewicz-Motowidło S. , Pikuła M. 
Published: November 2023

Background: Wound healing complications affect numerous patients each year, creating significant economic and medical challenges. Currently, available methods are not fully effective in the treatment of chronic or complicated wounds; thus, new methods are constantly sought. Our previous studies showed that a peptide designated as PDGF2 derived from PDGF-BB could be a promising drug candidate for wound treatment and that RADA16-I can serve as a release system for bioactive peptides in wound healing. Based on that, in this work, we designed a new self-assembling hydrogel RADA-PDGF2, connecting both peptides by a sequence specific for neutrophil elastase, and evaluated its activity in wound healing.Methods: The physicochemical properties of the designed scaffold were analyzed using transmission electron microscopy, atomic force microscopy, cryoSEM microscopies, and circular dichroism spectroscopy. The enzymatic cleavage was performed using human neutrophil elastase and monitored using high-performance liquid chromatography and MS spectroscopic techniques. The aforementioned techniques (HPLC and MS) were also used to assess the stability of the peptide in water and human plasma. The biological activity was analyzed on human skin cells using a colorimetric XTT test, collagen synthesis evaluation, and a migration assay. The biocompatibility was analyzed with LDH cytotoxicity assay and flow cytometric analysis of activation of immune cells. Finally, RADA-PDGF2 activity in wound healing was checked in a mouse dorsal skin injury model.Results: The analysis showed that RADA-PDGF2 can self-assemble, form a hydrogel, and release a bioactive sequence when incubated with human elastase. It shows pro-proliferative and pro-migratory properties and accelerates wound closure in the mouse model compared to RADA16-I. In addition, it is not cytotoxic to human cells and does not show immunogenicity. RADA-PDGF2 seems to be a promising drug candidate for wound management.
Proteomic and metabolomic analyses uncover integrative mechanisms in Sesuvium portulacastrum tolerance to salt stress
Authors: Cao Dingding , Zhang Wenbin , Yang Nan , Li Ziling , Zhang Chaoyue , Wang Dan , Ye Guiping , Chen Jianjun , Wei Xiangying 
Published: November 2023

IntroductionSalt stress is a major constraint affecting crop productivity worldwide. Investigation of halophytes could provide valuable information for improving economically important crops to tolerate salt stress and for more effectively using halophytes to remediate saline environments. Sesuvium portulacastrum L. is a halophyte species widely distributed in tropical and subtropical coastal regions and can absorb a large amount of sodium (Na). This study was to analyze S. portulacastrum responses to salt stress at morphological, physiological, proteomic, and metabolomic levels and pursue a better understanding of mechanisms behind its salt tolerance. MethodsThe initial experiment evaluated morphological responses of S. portulacastrum to different concentrations of NaCl in a hydroponic system, and subsequent experiments compared physiological, proteomic, and metabolomic changes in S. portulacastrum after being exposed to 0.4 M NaCl for 24 h as immediate salt stress (IS) to 14 days as adaptive salt stress (AS). Through these analyses, a working model to illustrate the integrative responses of S. portulacastrum to salt stress was proposed.ResultsPlants grown in 0.4 M NaCl were morphologically comparable to those grown in the control treatment. Physiological changes varied in control, IS, and AS plants based on the measured parameters. Proteomic analysis identified a total of 47 and 248 differentially expressed proteins (DEPs) in leaves and roots, respectively. KEGG analysis showed that DEPs, especially those occurring in roots, were largely related to metabolic pathways. Root metabolomic analysis showed that 292 differentially expressed metabolites (DEMs) occurred in IS plants and 371 in AS plants. Among them, 20.63% of upregulated DEMs were related to phenolic acid metabolism. DiscussionBased on the integrative analysis of proteomics and metabolomics, signal transduction and phenolic acid metabolism appeared to be crucial for S. portulacastrum to tolerate salt stress. Specifically, Ca2+, ABA, and JA signalings coordinately regulated salt tolerance in S. portulacastrum. The stress initially activated phenylpropanoid biosynthesis pathway through Ca2+ signal transduction and increased the content of metabolites, such as coniferin. Meanwhile, the stress inhibited MAPK signaling pathway through ABA and JA signal transduction, which promoted Na sequestration into the vacuole to maintain ROS homeostasis and enhanced S. portulacastrum tolerance to salt stress.
Spatial and temporal regeneration patterns within gaps in the primary forests vs. secondary forests of Northeast China
Authors: Wu Danni , Lu Deliang , Zhu Jiaojun , Ge Xiaowen , Zhang Jinxin , Lin Litao , Wang Xiaoyu , Liu Huaqi , Zhang Guangqi 
Published: November 2023

Forest gaps play an important role during forest succession in temperate forest ecosystems. However, the differences in spatial distribution and replacement patterns of woody plants (trees and shrubs) between primary and secondary forests remain unclear during the gap-filling processes, especially for temperate forests in Northeast China. We recorded 45,619 regenerated trees and shrubs in young gaps (&lt;10 years), old gaps (10~20 years), and closed forest stands (i.e., filled gaps) in the primary broadleaved Korean pine (Pinus koraiensis Sieb. Rt Zucc.) forests vs. secondary forests (degraded from primary forests). The gap-filling processes along horizontal (Cartesian coordinate system) and vertical (lower layer: 0~5 m, medium layer: 5~10 m, and upper layer: &gt;10 m) dimensions were quantified by shade tolerance groups of trees and shrubs. We found that gap age, competition between species, and pre-existing regeneration status resulted in different species replacement patterns within gaps in primary vs. secondary forests. Gap formation in both primary and secondary forests increased species richness, with 33, 38, 39, and 41 in the primary closed stands, primary forest gaps, secondary closed stands, and secondary forest gaps, respectively. However, only 35.9% of species in primary forest gaps and 34.1% in secondary forest gaps successfully reached the upper layer. Based on the importance values (IVs) of tree species across different canopy heights, light-demanding trees in the upper layer of the secondary forests were gradually replaced by intermediate and shade-tolerant trees. In the primary forests, Korean pine exhibited intermittent growth patterns at different canopy heights, while it had continuous regeneration along vertical height gradients in the secondary forests. The differences in Korean pine regeneration between the primary and secondary forests existed before gap formation and continued during the gap-filling processes. The interspecific competition among different tree species gradually decreased with increasing vertical height, and compared to the primary forests, the secondary forests showed an earlier occurrence of competition exclusion within gaps. Our findings revealed the species replacement patterns within gaps and provided a further understanding of the competition dynamics among tree species during the gap-filling processes.
Exploring moral algorithm preferences in autonomous vehicle dilemmas: an empirical study
Authors: Sui Tingting 
Published: November 2023

IntroductionThis study delves into the ethical dimensions surrounding autonomous vehicles (AVs), with a specific focus on decision-making algorithms. Termed the “Trolley problem,” an ethical quandary arises, necessitating the formulation of moral algorithms grounded in ethical principles. To address this issue, an online survey was conducted with 460 participants in China, comprising 237 females and 223 males, spanning ages 18 to 70.MethodsAdapted from Joshua Greene’s trolley dilemma survey, our study employed Yes/No options to probe participants’ choices and Likert scales to gauge moral acceptance. The primary objective was to assess participants’ inclinations toward four distinct algorithmic strategies—Utilitarianism, Rawlsianism, Egoism, and a Hybrid approach—in scenarios involving AVsResultsOur findings revealed a significant disparity between participants’ preferences in scenarios related to AV design and those focused on purchase decisions. Notably, over half of the respondents expressed reluctance to purchase AVs equipped with an “egoism” algorithm, which prioritizes the car owner’s safety. Intriguingly, the rejection rate for “egoism” was similar to that of “utilitarianism,” which may necessitate self-sacrifice.DiscussionThe hybrid approach, integrating “Utilitarianism” and “Egoism,” garnered the highest endorsement. This highlights the importance of balancing self-sacrifice and harm minimization in AV moral algorithms. The study’s insights are crucial for ethically and practically advancing AV technology in the continually evolving realm of autonomous vehicles.
Dual burden of chronic physical conditions and mental disorders: Findings from the Saudi National Mental Health Survey
Authors: Altwaijri Yasmin , Al-Habeeb Abdulhameed , Al-Subaie Abdullah , Bruffaerts Ronny , Bilal Lisa , Hyder Sanaa , Naseem Mohammad Talal , Alghanim Abdullah J. 
Published: November 2023

IntroductionComorbidities of mental disorders and chronic physical conditions are a common medical burden reported among Western countries. National estimates of such comorbidities among the general population of Arab countries like Saudi Arabia are unknown. This study examined the prevalence of lifetime chronic physical conditions among the Saudi general population with DSM-IV 12-month mental disorders, and the associations with disability in the Kingdom of Saudi Arabia (KSA).MethodsThe Saudi National Mental Health Survey, a cross-sectional household study – part of the World Mental Health (WMH) Survey Consortium – was conducted between 2013–2016 in the KSA, with 4,001 Saudi citizens aged 15–65 (response rate 61%). The World Health Organization Composite International Diagnostic Interview 3.0 was used to assess prevalence of lifetime chronic physical conditions and 12-month mental disorders; disability was measured in terms of days out of role.ResultsThe prevalence of any comorbid 12-month mental disorder among those with chronic physical conditions was 24%. Major depressive disorder, social phobia, and adult separation anxiety disorder were the most common comorbid mental disorders across all chronic physical conditions. Gender, education, income, urbanicity, region, and employment were associated with the presence of any chronic physical condition. Respondents with mental / physical comorbidities had 2.97 days out of role (on average) in the last 30 days.ConclusionComorbidities of mental disorders and chronic physical conditions are common among Saudis. National efforts are needed to increase awareness of such comorbidities among the general population, and develop prevention and treatment services tailored to the needs of individuals at-risk for comorbidities.
Body weight, gonadectomy, and other risk factors for diagnosis of osteoarthritis in companion dogs
Authors: Graves Jessica L. , McKenzie Brennen A. , Koch Zane , Naka Alexander , Spofford Nathaniel , Morrison JoAnn 
Published: November 2023

ObjectiveThe aim of this study is to evaluate age, sex, body weight, breed, neuter status, and age at neutering as risk factors for diagnosis of osteoarthritis in companion dogs.AnimalsDogs seen as patients at Banfield Pet Hospital in the United States from 1998 to 2019 with a date of death in 2019. The final cohort consisted of 131,140 dogs.MethodsIn this retrospective cohort study, Cox proportional hazard models were used to test for associations between osteoarthritis incidence and age at baseline, sex, maximum body weight, maximum body condition score, neuter status, and age at neutering. The same model was used to test these associations in 12 representative breeds, chosen based on breed weight and sample size.ResultsOlder age, higher adult body weight, gonadectomy, and younger age at gonadectomy were significantly associated with higher risks of osteoarthritis in the total cohort and in all 12 breeds evaluated. Higher body condition scores and sex were also significantly associated with osteoarthritis but with minimal effect sizes in the overall cohort, and these risk factors were not consistently significant in all breeds tested.Clinical relevanceThese results will assist veterinarians in identifying dogs at higher risk for osteoarthritis and applying appropriate diagnostic, preventative, and treatment interventions. An understanding of potentially modifiable risk factors, such as body condition and neutering, will support evidence-based discussions with dog owners about risk management in individual patients.
COVID-19 Vaccination Among Diverse Population Groups in the Northern Governorates of Iraq
Authors: Gubari Mohammed Ibrahim Mohialdeen , Wadi Falah , Hama-Ghareeb Khalid Anwar , Fatah Fatah H. , Hosseini Mostafa , Wale Karzan Rafiq , Hipgrave David , Ali Sazan Raouf , Mahmood Shaho Osman , Ezzat Khadium Waleed , Mohammed Hayman Hasan , Jaafer Sara Feal , Al Saadi Najeeb Mohammed , Mohammed Kardar Anwar , Saeed Shireen Salih , Mohammad Mohammad Yousif , Khudhur Waleed Hamid , Wael Saleh Mohammed , Sheat Yousra Saadi , Ibrahim Khaldoon Khaleel , Nabeil Salah Marwa , Abdullah Abdulmonem Hazim , Omer Dashne Shamall , Ghafur Rafeeq Naseraldeen , Mustafa Kashma Ali , Faraj Aryan Othman , Ali Trifa Bhjat , Aziz Enayat Kamal , Wahab Ronak Assi , Ali Al-Jaf Ibrahim Ahmed , Hama Amin Nazanin Hama , Jaff Dina Dlshad , Bra Aram Mohammed , Ahmed Shwan Kanabi , Rasool Banaz Nabi , Jamal Fatimah Khalis , Mohammed Tango Dhahir , Azeez Maryam Arab 
Published: November 2023

Objectives: The present study was carried out to investigate COVID-19 vaccination coverage among populations of internally displaced persons (IDPs), refugees, and host communities in northern Iraq and the related underlying factors.Methods: Through a cross-sectional study conducted in five governorates in April–May 2022, 4,564 individuals were surveyed. Data were collected through an adapted questionnaire designed to gather data on participants.Results: 4,564 subjects were included (59.55% were 19–45 years old; 54.51% male). 50.48% of the participants (51.49% of host communities, 48.83% of IDPs, and 45.87% of refugees) had been vaccinated with at least one dose of COVID-19 vaccine. 40.84% of participants (42.28% of host communities, 35.75% of IDPs, and 36.14% of refugees) had been vaccinated by two doses, and 1.56% (1.65% of host communities, 0.93% of IDPs, and 1.46% of refugees) were vaccinated with three doses.Conclusion: Sociodemographic factors including age, gender, education, occupation, and nationality could affect vaccination coverage. Moreover, higher acceptance rate of vaccination is associated with belief in vaccine safety and effectiveness and trust in the ability of the vaccine to prevent complications.
Measurement of the Immunosuppressant Possession Ratio by Transplant Clinical Pharmacists Captures a Non-Adherence Associated With Antibody-Mediated Rejection
Authors: Chambord Jérémy , Chauveau Bertrand , Djabarouti Sarah , Vignaud Jean , Taton Benjamin , Moreau Karine , Visentin Jonathan , Merville Pierre , Xuereb Fabien , Couzi Lionel 
Published: November 2023

Our objective was to calculate an immunosuppressant possession ratio (IPR) to diagnose non-adherence at the time of antibody-mediated rejection (ABMR). IPR was defined as the ratio of number of pills collected at the pharmacy to the number of pills prescribed over a defined period. In a first cohort of 91 kidney transplant recipients (KTRs), those with an IPR &lt; 90% had more frequently a tacrolimus through level coefficient of variation &gt;30% than patients with an IPR = 100% (66.7% vs. 29.4%, p = 0.05). In a case-control study, 26 KTRs with ABMR had lower 6 months IPRs than 26 controls (76% vs. 99%, p &lt; 0.001). In KTRs with ABMR, non-adherence was more often diagnosed by a 6 months IPR &lt; 90% than by clinical suspicion (73.1% vs 30.8%, p = 0.02). In the multivariable analysis, only de novo DSA and 6 months IPR &lt; 90% were independently associated with ABMR, whereas clinical suspicion was not (odds ratio, 4.73; 95% CI, 1.17–21.88; p = 0.03; and odds ratio, 6.34; 95% CI, 1.73–25.59; p = 0.007, respectively). In summary, IPR &lt; 90% is a quantifiable tool to measure immunosuppressant non-adherence. It is better associated with ABMR than clinical suspicion of non-adherence.
Microstructural changes in Oxisols under long-term different management systems
Authors: Ngolo Aristides Osvaldo , Oliveira Fábio Soares de , Oliveira Maurílio Fernandes de , Fernandes Raphael Bragança Alves 
Published: November 2023

ABSTRACT
There has long been a discussion about the effects of soil management on its structure. Since changes can occur due to management and time of use, more accurate assessments can be achieved if carried out in long-term experiments. This study investigated the long-term effects of soil management on the physical quality of a Cerrado Oxisol (Latossolo Vermelho), focusing on microstructural changes. Micromorphology and computed tomography techniques were used to assess the soil's microstructure. The study compared areas under long-term and different soil management practices, including disc plowing, no-tillage, and disc harrow+subsoiler. A native Cerrado area was considered as the reference. Micromorphology revealed some changes in the pedological features of soil aggregates, but the granular structure showed good resistance even after two decades of use and management. It also indicated a decrease in larger pores and an increase in the surface soil layer micropores for the disc plowing and no-tillage treatments. These results were consistent with traditional laboratory evaluations of soil porosity. Computed tomography was limited due to increased soil bulk density in the cultivated treatments, but it showed potential for assessing soil porosity and pore connectivity. We concluded that micromorphology effectively identifies microstructural changes in Oxisols with small and strong granular structures, and the granular soil aggregates displayed resilience even after long-term management. The micromorphometric evaluation corroborates with traditional methods and suggests loss of pores associated with the disc harrow+subsoiler treatment.
PERANCANGAN SISTEM INFORMASI PEMESANAN TIKET BUS PADA PO. HANDOYO BERBASIS ONLINE
Authors: Setiawan Sianturi Julianto Simatupang, 
Published: November 2023

Di tengah perkembangan ilmu pengetahuan dan teknologi yang sangat pesat ini, pemesanan tiket bus masih banyak dilakukan secara manual, cara ini kurang efektif dan efisien. Pada umumnya, prosedur atau metode yang digunakan saat ingin membeli tiket bus adalah datang langsung ke loket yang ada di terminal ataupun pada agen. Permasalahan yang sering terjadi adalah penumpang sering kehabisan tiket pada saat datang ke loket karena kurangnya informasi tentang jadwal keberangkatan. Oleh karena itu, penelitian ini mengangkat permasalahan mengenai sistem pemesanan tiket bus yang sampai saat ini masih dilakukan secara manual. Tujuannya untuk membangun sistem informasi pemesanan tiket berbasis online, dengan menerapkan siklus hidup pengembangan sistem yaitu metode waterfall. Dengan adanya sistem informasi pemesanan tiket bus ini diharapkan dapat menyelesaikan masalah-masalah yang terjadi dan dapat meningkatkan efisiensi dan efektifitas agar pelayanannya menjadi lebih baik dibanding dengan sistem yang terdahulu.