bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical assessment of the concentration of anti-Muller hormone and biochemical parameters of the blood of donor cows in the luteal phase of the cycle
Authors: Kozitcyna A. I. , Karpenko L. Yu. , Nikitin G. S. , Achilov V. V. , Olontsev V. A. , Semenova V. S. 
Published: December 2023

Increasing the productivity of cows in the modern world is a key aspect of animal husbandry. Embryo transplantation in cows makes it possible to increase the production of genetically valuable animals and improve the breeding qualities of the herd. One of the methods for determining the activity and reserve of the ovaries is the determination of anti-Muller hormone in the blood. AMH plays an important role in the regulation of reproductive function in animals, including cows. High levels of AMH may indicate a good reserve capacity of the ovaries, which may be useful when choosing animals for breeding. In the presented study, the bio-chemical parameters of the blood of 9 Holstein cows in the luteal phase were analyzed. A direct correlation of a high degree (0.89 and 0.89, respectively) was found between the AMH level indicator and urea and urea nitrogen indicators. There was a direct noticeable correlation between the AMH level and the serum creatinine level (0.63). Also, when assessing the indicators of mineral metabolism, strong correlations were revealed: negative when comparing the level of AMH with the level of calcium (-0.71) and positive when comparing the level of AMH with the level of phosphorus (0.78). The regulation of the synthesis of AMH itself and its metabolic effects have been studied little, there is only a limited amount of data on the relationship of the hormone with other factors of autocrine and paracrine regulation. For a more accurate understanding of this correlation and its significance, additional research is needed.
Characteristics of erythropoiesis in sows during different periods of the production cycle
Authors: Voronina О. А. , Zaitsev S. Yu. 
Published: December 2023

The tactics of periodic monitoring of the hematology of sows allows timely measures to prevent anemia, which inevitably occurs, since the targeted screening and selection of industrial breeds are aimed at multiple births, which increases the risks of anemia during pregnancy and suckling period. With periodic and constant monitoring, it is possible to deduce individual reference intervals of hematological indicators for a particular animal and control the response to compensatory measures that directly affects the yield of viable piglets and their survival rate. The purpose of this study was to study the number of red blood cells, hemoglobin level, hematocrit, average volume of red blood cells and erythrocyte indices depending on the production cycle of the sow. The groups were formed from sows on the 80th day of pregnancy (group 1, n=5), after farrowing (group 2, n=5) and before weaning piglets (group 3, n=5). The Vet ABCTM analyzer (Horiba Medical Poland) was used. Counting of reticulocytes by “supravital staining” with a solution of “brilliant cresyl blue”. Statistical processing in the Microsoft Excel program (Data Analysis package), evaluation of the reliability of differences based on the Mann—Whitney U criterion. As a result, it was found that the average volume of red blood cells increases with a decrease in their number and hemoglobin concentration, which is quite natural and indicates a regenerative response with the appearance of reticulocytes in the blood. The correlation coefficient between erythrocytes and the average volume of erythrocytes was r=-0.53 (r2=0.28, p=0.01), between the average volume of erythrocytes and reticulocytes r=-0.50 (r2=0.25, p=0.05). There were no significant differences among the groups in reticulocytes and the average volume of erythrocytes. The dynamics and intensity of regeneration are moderate in all three groups.
Dependence of coagulation activity of chicken blood IN VITRO on temperature
Authors: Fomina L. L. , Berezina D. I. , Kulakova T. S. , Modanova K. E. 
Published: December 2023

The paper presents the results of examine the relationship between blood plasma incubation temperature and the hemostasis parameters in chickens. The purpose of this research was to study of the blood coagulation parameters in chickens during hypo-, normo- and hyperthermia in vitro. In addition, to assess the possibility of using a coagulometer to identify hemostasis activity and the use of hemostatic reactions in chickens as models for human medicine. The Vologda region was where the study was conducted. The coagulogram was examined for the following parameters: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen activity, and antithrombin III activity (AT-III). It was found that at 46° C activation of blood clotting occurs, manifested as an acceleration of the TT by 29% (13 seconds), PT by 72% (111 seconds), and decrease in the antithrombin III activity by 6% (1.5 seconds) compared to normothermia. The parameters of secondary hemostasis also responded to hypothermia (18 °C) with hypercoagulation. It was an acceleration of PT of 81% (125 sec), an increase in fibrinogen activity by 77% (84 sec) and a decrease in AT-III activity by 55% (14 sec), with the exception of thrombin time, which was extended by 70% (104 seconds). There were no significant changes in the APTT at different temperatures. The correlation-regression and one-factor analysis of variance revealed a significant correlation between the temperature and the TT and the fibrinogen activity, where the coefficients of determination for which were 71% and 39%, respectively. Plasma hemostasis activity measured on a coagulometer (at 37 °C) showed no significant differences from those measured at 43 °C, except for prothrombin time, which was 71.51 seconds (47%) less than that measured at 43 °C.
Qualitative indicators of sperm from producing goats before and after cryopreservation
Authors: Korochkina E. A. , Trifonova A. V. , Finageev E. Yu. , Glavatskaya D. E. , Pushkina V. S. 
Published: December 2023

Currently, artificial insemination, as one of the types of assisted reproductive technologies, is widely used in dairy and beef cattle breeding. The same cannot be said about such a promising direction in the agricultural industry as goat breeding. One of the limiting factors is the negative impact of low temperature on the morphofunctional charateristics of sperm of breeding goats. The purpose of this research was to test a protocol of sperm cryopreservation of stud goats with modification of prepreparation and subsequent assessment of the quality indicators of sperm before and after its deep freezing. A comprehensive assessment of sperm quality (volume, concentration, morphology, motility) of goats (n=10) was carried out using generally accepted methods and protocols. The assessment of sperm quality indicators included five stages: after sperm collection, two hours after cooling, after thawing: 0 hours, 1 and 2 hours. According on the obtained results, the sperm of breeding goats ha low cryoresistance. After cryopreservation (0, 1 and 2 hours after thawing), there is an increase in the number of sperm with tail damage by 7.5% (p≤0.05), 15.5% and 21.8% (p≤0.01), and also a decrease in the number of progressively moving sperm by 1.4; 1.6 and 2.5 times (p≤0.01) compared with the results of the assessment 0 hours after collection. The use of a deep two-phase sperm freezing protocol allows maintaining the viability of sperm with a progression of movements equal to 54.2±5.1% and a number of morphologically normal sperm equal to 64.1±1.9%. In this case, the prepreparation of sperm for the cryopreservation process (current protocol) includes sperm centrifugation (mode: 7000 rpm for 15 minutes), removal of seminal plasma, dilution 1:4 (OptiXcell diluent), cooling (4 hours at 4℃); sperm cryopreservation protocol: 1. immersion of goblets with paillettes 4 cm above liquid nitrogen for 7 minutes; 2. complete immersion in liquid nitrogen.
The effect of the mitochondrial-directed antioxidant Mito-TEMPO on the quality of rooster sperm in the cryopreservation and lyophilization cycle
Authors: Kurochkin A. A. , Stanishevskaya O. I. , Silyukova Y. L. , Pleshanov N. V. 
Published: December 2023

In reproductive technologies applied in animal husbandry, using lyophilized semen is only possible with ICSI technology, due to complete loss sperm motility. However, the anatomical structure’s peculiarities and development of avian oocyte not allows use this technology. Strategies aimed at preserving kinetic apparatus of lyophilized sperm are the most relevant for birds. Increasing mitochondrial activity of cells due to its connection with energy metabolism and sperm motility may be one of the ways to solve this problem. However, similar studies on freeze-dried bird semen haven’t been conducted on this moment. The present study assessed effect of mitochondria-targeted antioxidant Mito-TEMPO on viability, mitochondria’s membrane potential and generation of intracellular hydrogen peroxide by rooster spermatozoa in a cryopreservation and lyophilization cycle. Proportion of cells with intact plasma membrane in lyophilized semen was higher using extenders containing an antioxidant. Addition of Mito-TEMPO at concentration of 40 µM to diluents T-20 and T-30 for the purpose of cryopreservation of semen with subsequent freezedrying allowed to increase proportion of cells with intact plasma membrane in lyophilized semen in comparison to control diluents (6.12 ± 0.11 % versus 10.19±0.46% and 13.19±1.30% versus 15.23±1.13%, respectively, p˂0.05). Effective concentration of Mito-TEMPO in diluents intended only for semen cryopreservation was significantly lower - 5 µM. Proportion of cells with intact plasma membrane was 47,97±0,64% versus 55,85±1,69% and 47,25±2,97% versus 63,21±1,78%, respectively, p˂0, 05. Dose-dependent effect of trehalose in cryoprotective diluents has been proven.
The effect of the concentration of β-hydroxybutyrate and glucose in the blood on the reproductive function and milk productivity of cows
Authors: Nikolaev S. V. 
Published: December 2023

The intensity of metabolic processes in highly productive cows is associated with metabolic disorders and the development of dystrophic changes in the body. The paper evaluates the effect of the concentration of β-hydroxybutyrate and glucose in the blood of new-bodied cows on the indicators of reproductive ability and milk productivity. To do this, venous blood was obtained from cows of different ages after childbirth, where the concentration of glucose and β-hydroxybutyrate was determined. Depending on the level of glucose and ketone bodies, animals were retrospectively differentiated into groups and the severity of economically useful signs was determined. It was found that cows with a glucose level above 3.3 mmol/l required 0.6 more inseminations (P≤0.01), compared with animals whose indicators were in the range of 2.2...3.3 mmol/l. At the same time, the greatest difference in the multiplicity of insemination was present in animals 3...7 lactation (1.2 inseminations per fertilization; P≤0.001). The period from calving to fertilization in cows with a high glucose content was longer by 39.4 days (P < 0.05), while the maximum deviation was found among animals older than the 2nd lactation (by 43.4%; P< 0.05). An increase in the level of β-hydroxybutyrate over 0.7 mmol/l was also accompanied by a deterioration in reproductive function. Thus, the lengthening of the infertility period for all lactation was 21.2 days (P≤0.05). The most pronounced negative effect on reproductive function in ketonemia was observed in first-heifers and cows of the second lactation. So, after the first birth, the lengthening of the period from calving to fertilization was 42.0 days (P < 0.05), and after the second lactation - 29.2 days (P < 0.05). Thus, an increase in the level of glucose and β-hydroxybutyrate in the blood negatively affects, first of all, the reproductive function of cows, and to a lesser extent, the indicators of milk productivity.
Laboratory methods for determining the marker of proliferative activity of Ki-67 in the placenta of cows in complicated pregnancy and pathological childbirth
Authors: Avdeenko V. S. , Makavchik S. A. , Safronov D. I. , Moiseeva K. A. 
Published: December 2023

In modern high-tech dairy enterprises, the problem of perinatal diseases in highly productive dairy cattle is acute. The current study presents the distribution of extracellular matrix in cows during physiological pregnancy ending in spontaneous labor (FB/SR) and complicated pregnancy with symptoms of preeclampsia/eclampsia ending in pathological labor (OB/PR) using histological, histochemical (HC) and immunohistochemical (IHC) research methods. Biopsy material of placentas was obtained from 10 cows during labor: from 5 cows with FB/SR and from 5 cows with OB/PR. Samples of placentas from cows with FB/SR and OB/PR were subjected to standard histological procedures. Paraffin sections of the placenta, 5 µm thick, were mounted on glasses treated with poly-L-lysine (Menzel). Using IHC studies, the immunolocalization of the Ki-67 biomarker was studied. A histochemical study in the placentas of cows with FB/SR revealed the presence of a large number of small capillaries both in the villi of the cotyledons and in the caruncular crypts, as well as an equilibrium ratio of mesenchymal and epithelial structures. The reaction to vimentin showed degradation of fibroblasts. In the lumen of the crypts, only structureless masses and a small number of macrophagelike cells were recorded. In addition, in the central part of the unchanged villi, a sharp decrease in the number of small capillaries was established, as well as a reduction in the terminal chorionic villi and a decrease in the vascularization of the remaining villi. Immunolocalization of the content of intermediate biomarkers showed the content of a large amount of Ki-67 in the cells of the crypt lining. During pregnancy OB/PR, a decrease in the vascularization of chorionic villi in the cotyledons and degradation of cytokeratin filaments involved in the formation of cellular contacts between the cytotrophoblasts of the chorionic villi of the cotyledons of the fetal part of the placenta and the epithelial cells of the crypts of the caruncles of the maternal part of the placenta have been demonstrated, which will make it possible in the future to develop an enzyme-linked immunosorbent assay (ELISA) as a rapid – test for diagnosing reproductive pathologies in dairy cattle. Pronounced expression of the Ki-67 protein was found in the cells lining the crypts of the caruncles, as well as in the cytoplasmic membrane of the villous cytotrophoblasts. In the placenta of cows whose pregnancy and childbirth were complicated by pathology, the proliferative activity of the cells lining the uterine crypts remained at a physiological level, while individual chorionic villi were completely reduced, and in those that remained, the Ki-67 expression index was 36.94%.
The genetic basis of the sperm quality of stallions
Authors: Nikitkina E. V. 
Published: December 2023

One of the important factors affecting the reproductive function is the genotype of the animal. The method of genomic selection is based on the use of polymorphic single nucleotide substitutions (SNP) as markers for determining the value of the genotype of an animal or plant. The formation and functioning of the reproductive system of animals occurs as a result of the coordinated interaction of a wide range of genes. The search for genomic associations of GWAS (Genome-Wide Association Studies) with signs of fertility will allow further selection of animals based on these indicators at the genomic level. Sperm was obtained with the help of mares in hunting and on a phantom using an artificial Hannover vagina (Minitube, Germany). The sperm of each stallion was collected at least three times. The semen quality of 96 stallions of different breeds (Arabian, Akhal-Teke, Trakenen, thoroughbred horse, Oryol trotter, French trotter, Soviet heavy horse, Shetland pony, Welsh pony, horses of different breeds) was analyzed. High variability of sperm quality indicators was revealed. For example, the concentration of sperm in the ejaculate varied from 59 to 514 million /ml, progressive mobility from 20 to 87%, and the number of cells without damage from 21.6 to 95.6%. Such variability of the studied features makes it possible to assume that their formation in the process of ontogenesis occurs under the influence of genetic factors. The GWAS analysis of ejaculate volume, concentration and motility of stallion sperm revealed potential candidate genes associated with these indicators. The associations identified in our study are promising candidates for further identification of the biological mechanisms underlying the quality of stallion sperm.
Investigation of metabolism and functioning of the antioxidant system in cats with tripelphosphate urolithiasis
Authors: Ushakova T. M. , Derezina T. N. , Chichilenko V. S. 
Published: December 2023

Urolithiasis is a chronic disease of the urinary tract of a polyethological nature, accompanied by the formation of uroliths and sand in the kidneys and urinary tract, as well as signs of dysuria, pollakiuria, ischuria, urinary colic, periodic hematuria and crystalluria. The purpose of our research was to study the metabolism and functioning of the antioxidant system in cats with tripelphosphate urolithiasis. The results of our study of the metabolism and antioxidant system of sick animals indicated the development of disorders of redox regulation of cells in tripelphosphate erythrolithiasis in cats. A disorder of liver functional activity was detected (AST - 41.74±5.35 Units/l and 49.30±6.74 Units/L; ALT - Units/l and Units/L; ALKP - Units/L and Units/L; BILT - mmol/l and mmol/l; BILD - mmol/l and 1.62mmol/L; AAR – 0.49±0.03 and 0.54±0.02) and violation of cell redox regulation (MDA - 1.29±0.08 nmol/ml and 1.40±0.10 nmol/ml; SOD - 265.90±12.08 Units/ml and 280.05±10.43 Units/ml; coenzyme Q10 - 579.03±19.06 ng/ml and 505.42±23.10 ng/ml;8-OHdG - 0.15±0.01 ng/ml and 0.13±0.02ng/ml; ascorbic acid - 5.03±0.91 mcg/ml and 4.70±1.03 micrograms/ml; TAS - 1.25±0.07 mmol/l and 1.23±0.05 mmol/L; GTP - 6234.00±24.82 Units/g Hb and 6015.12±27.56units/g Hb) in cats with tripelphosphate urolithiasis. It was found that there is a direct close correlation between the level of liver transaminases and the activity of antioxidant enzymes, as well as between the level of circulating kidney biomarkers and the level of the main markers of oxidative stress in urolithiasis struvite type in cats, which allows us to expand the understanding of this disease and use these data in the implementation of therapeutic measures.
Имеет ли русская баба-Яга отношение к греческой Ехидне?
Authors: Valentsova M. 
Published: December 2023

The article discusses the discrepancy between the meaning of the mythological image of the East Slavic Baba-Yaga and the etymological meaning of the name yaga / yazya in the Slavic languages. Alternative versions of the yaga’s etymology are cited that raise it to an ancient Indo-European name of the snake (and hedgehog). Also folklore plots confirming such semantics are denoted in which Yaga is interchangeable with the Snake. A hypothesis offered by O. A. Cherepanova about possible contacts of Slavs with Greeks and Iranians in the Northern Black Sea area is developed and a possible source of the Slavic Baba-Yaga image is considered: the Scythian legends about the serpentine goddess ancestress of Scythians as described by Herodotus based on Greek images of snake demons, including Echidna.
В статье обсуждается несоответствие значения мифологического образа восточнославянской бабы-Яги этимологическому значению имени яга / язя в славянских языках. Приводятся альтернативные версии этимологии имени яга, возводящие его к древнему индоевропейскому названию змеи (и ежа), а также подтверждающие эту семантику фольклорные сюжеты, в которых Яга взаимозаменяема со Змеей. Развивается предложенная О. А. Черепановой гипотеза о возможном контакте славян с греками и иранцами в Северном Причерноморье и как возможный источник образа славянской бабы-Яги рассматриваются скифские легенды о змееногой богине-прародительнице скифов в изложении Геродота, основанные на греческих образах змееподобных демонов, включая Ехидну.
Modern Greek Demons of the Twelve Days of Christmas: kalikandzars, skalapundars, planitars, pagans, pizilas
Authors: Климова К.А. 
Published: December 2023

Статья посвящена описанию новогреческих святочных демонов каликандзаров, в том числе их локальных номинаций, генезису мифологического персонажа, их внешнего облика, времени и места появления и трансформации традиционных руральных народно-мифологических представлений в современном греческом городском пространстве.
The article is devoted to the description of the modern Greek Christmas demons Kalikandzars, including their local nominations, description of the genesis and appearance, time and place of the appearance and transformation of their traditional rural folk-morphological representations in the modern Greek urban space.
On suicide: the meaning of good death in Ancient Greece and the desperate act of suicide in Modern Greek Literature. The case of the Suicidal Neohellenic writer Periklis Giannopoulos (1869–1910)
Authors: Δαπουδάνη Α.Μ. 
Published: December 2023

Η αυτοχειρία εξετάζεται μέσα από το πρίσμα της Αρχαίας Ελληνικής Φιλοσοφίας και των Αρχαίων θεατρικών δραμάτων. Ερευνάται η περίπτωση του Νεοέλληνα Λογοτέχνη Περικλή Γιαννόπουλου που επέλεξε χωρίς τον παραμικρό δισταγμό την πράξη της αυτοκτονίας ως δείγμα αντίστασης στην υποταγή και στα τυφλά προστάγματα της μοίρας. Η αυτοχειρία αποδείχθηκε τελικά και για τον Περικλή Γιαννόπουλο ως και για όλους τους Νεοέλληνες αυτόχειρες λογοτέχνες ως μία πράξη ελευθερίας και αποτίναξης κάθε τυραννικού δεσμού και επιβεβλημένου εξολοθρευτικού συμβιβασμού. Η μοίρα όλων αυτών των αυτοχείρων όπως πολύ σωστά παρατηρεί η ποιήτρια Κική Δημουλά: Λέγεται μοίρα της εξαίρεσης, της σπανιότητας [Δημουλά 2022: 69]. Πρόκειται για χαρισματικές προσωπικότητες με υψηλή νοημοσύνη και ευαισθησία που γεννήθηκαν με θείο δικαίωμα Jure Divino και ούτε ακόμη και ο ίδιος ο χρόνος μπορεί να τις αφανίσει.
Suicide is examined through the prism of Ancient Greek Philosophy and Ancient dramas. The case of the Neo-Greek writer Pericles Giannopoulos who chose without the slightest hesitation the act of suicide as a sign of resistance to submission and the blind decrees of fate is investigated. Suicide was finally proven for Periklis Giannopoulos as well as for all the Neo-Greek writers who committed suicide as an act of freedom and of shaking off every tyrannical bond and imposed exterminating compromise. The fate of all those who committed suicide as the writer Kiki Dimoula rightly observes: It is called the fate of the exception, of the rare [Dimoula 2022: 69]. These are charismatic personalities with high intelligence and sensitivity who were born with divine right Jure Divino and not even time itself can destroy them.