bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infiltrated macrophages aggravate TMJOA chronic pain via Piezo2 in IB4 + - TG neurons
Authors: Jia Xueke , Liu Xin , Zhu Taomin , Ma Xiaohan , Chen Ruiming , Li Huimin , Feng Yaping , Zheng Liwu , Liu Yu , Ke Jin 
Published: January 2024

Abstract

Temporomandibular joint osteoarthritis (TMJOA) chronic pain is one of the orofacial pains that result in limitations in chewing function and a decline in quality of life. Currently, therapies for TMJOA chronic pain are inadequate due to a lack of understanding of its underlying mechanism. Recent research has shown that macrophages in the ganglia play a role in the development of chronic pain. Piezo2, an ion channel for nociception, has potentially been discovered in ganglia neurons. In this study, we found that infiltrated macrophages, rather than tissue-resident macrophages in trigeminal ganglia (TGs), are involved in monosodium iodoacetate (MIA)-induced TMJOA chronic pain in rats. The number of infiltrated macrophages is positively correlated with the elevation of Piezo2 in the trigeminal ganglion (TG) neurons of TMJOA rats. Consistently, depletion of infiltrated macrophages through Cl2MDP tail intravenous injections leads to a down-regulation of Piezo2 in TG neurons. Additionally, overexpression of Piezo2 in TG neurons through adeno-associated virus 9 (AAV9)-Piezo2 targeting rats' neurons intracerebral injection reverses the alleviation effect of infiltrated macrophages depletion on TMJOA chronic pain in rats. Furthermore, infiltrated macrophages primarily mediate the expression of Piezo2 in IB4+-TG neurons of TMJOA chronic pain rats. Moreover, an ex vivo study demonstrates that IL-1β and TNF-α, the main pro-inflammatory cytokines secreted by infiltrated macrophages, induce the activation of rat Dil+-TG neurons by up-regulating Piezo2. This study demonstrates that infiltrated macrophages contribute to MIA-induced TMJOA chronic pain by upregulating the expression of Piezo2 in IB4+-TG neurons, providing new insights into the mechanism of TMJOA chronic pain.
Upscaling irradiation protocols of Aedes albopictus pupae within an SIT Programme in Reunion Island
Authors: Marquereau Lucie , Yamada Hanano , Damiens David , Leclercq Antonin , Derepas Brice , Brengues Cécile , Dain Brice William , Lejarre Quentin , Proudhon Mickael , Bouyer Jeremy , Gouagna Louis Clément 
Published: January 2024

Abstract
The implementation of the sterile insect technique against Aedes albopictus relies on many parameters, in particular on the success of the sterilization of males, released into the target area in overflooding numbers to mate with wild females. Achieving consistent sterility levels requires efficient and standardized irradiation protocols. Here, we assessed the effects of exposure environment, density of pupae, irradiation dose, quantity of water and location in the canister on the induced sterility of male pupae. We found that irradiation of 2000 pupae in 130 ml of water and with a dose of 40 Gy was the best combination of factors to reliably sterilize male pupae with the specific irradiator used in our control programme, allowing the sterilization of 14000 pupae per cycle. The location in the canister had no effect on induced sterility. The results reported here allowed the standardization and optimization of irradiation protocols for an SIT programme to control Ae. albopictus on Reunion Island, which required the production of more than 300,000 sterile males per week.
The Combined Improvement of Acute Exercise Fatigue through Hyperbaric Oxygen and Astaxanthin Under the Background of Artificial Intelligence
Authors: Zhang Zheng , Liu Meng , Huang Xizhang , Gao Binghong 
Published: January 2024

Abstract

Objective this work explored the application research of artificial intelligent (AI) algorithms in the oral and psychological rehabilitation of patients with chronic diseases through deep learning and hyperbaric oxygen combined with astaxanthin therapy. Materials and Methods a model was constructed using convolutional neural network (CNN) combined with multivariate gaussian distribution (MGD) to comprehensively analyze multi-mode health data (numeric, textual, and image). The performance of the CNN model and the CNN + MGD model was compared in terms of accuracy (Acc), area under the receiver operating characteristic curve (AUC), precision (Pre), and recall rate (Recall). Shanghai active football players were selected as the research subjects and divided into a control group, a hyperbaric oxygen intervention group, and a hyperbaric oxygen + astaxanthin intervention group. An AI system was adopted to analyze the electronic health data of patients, assess the efficacy of hyperbaric oxygen in eliminating acute fatigue in patients with chronic diseases, and explore its relationship with oral and psychological health. Results the Acc, AUC, Pre, and Recall of the CNN + MGD model were significantly higher than those of the three-dimensional CNN model (P < 0.05), demonstrating superior performance. Hyperbaric oxygen + astaxanthin treatment significantly promoted the elimination of acute fatigue in patients (P < 0.05), accelerated the recovery of blood oxygen saturation and muscle oxygen saturation, and significantly affected the degradation of blood lactate (P < 0.05). As a result, it rapidly reduced blood oxygen saturation and muscle oxygen saturation after acute fatigue exercise. The oral health-related quality of life in patients with chronic diseases was positively correlated with depression and anxiety levels. Psychological intervention can be conducted in clinical treatment to improve the treatment effect. Conclusion this work comprehensively evaluated the oral and psychological rehabilitation effects in patients with chronic diseases through deep analysis of electronic health data using intelligent algorithms that integrate deep learning with hyperbaric oxygen and astaxanthin therapy. The results suggested that hyperbaric oxygen + astaxanthin treatment not only significantly improved blood indicators but also exhibited a positive correlation with oral health-related quality of life and psychological health, providing a new treatment approach for patient recovery. Therefore, the research results were of great significance for improving patient quality of life and enhancing clinical treatment efficacy.
Quantifying collective enthusiasm and fan engagement: A case study of the Japanese professional baseball league
Authors: Otomo Naofumi , Mizuno Makoto , Sasahara Kazutoshi , Sano Yukie 
Published: January 2024

Abstract

Although collective behaviour in human societies sometimes leads to irrational outcomes, its quantitative and real-time measurement has long been challenging. In recent years, the rise of social media has led to new studies on collective attention, focusing on specific events within collective behaviour including elections and sports. In such events, quantifying collective attention using quantitative methods with a real-time nature has become possible. In collective attention, burst phenomena, rapid increase in posts, and interaction between participants have been largely examined. Conversely, more microscopic phenomena such as the participants’ identity and the exact words they post, still require thorough understanding. Therefore, enthusiasm in this study is defined as the state in which broad participants direct their narrow attention and quantify it. Specifically, we propose an indicator that represents the state in which a large number of people participate and their attention is focused locally. On testing the indicator using Twitter data collected through the hashtags of various Japanese professional baseball teams, positive and negative events including championships and player injuries generate enthusiasm, respectively. In an analysis that distinguishes between own-team fans and fans of other teams, collective attention involving even other team fans is revealed to occur for significant enthusiasm. Using the micro perspective defined in this study, which considers the local attention of enthusiasm, the mechanism of collective attention is clarified. Furthermore, clues for quantifying the real-time origins of collective behaviour in human society with both positive and negative aspects are provided.
Impact of COVID-19 Lockdown Measures on Mother and Child Health – The case of Ghana
Authors: Koka Eric , Asante-Poku Adwoa , Adjei Daniel , Sebbie Diana , Siam Ishaque , Asare Prince , Yeboah-Manu Dorothy , Gadzekpo Audrey , McCoy David , Narh Emmanuel 
Published: January 2024

Abstract

Background The lockdown measures in response to the coronavirus disease (COVID-19) have led to a wide range of unintended consequences for women and children. Until the outbreak of COVID-19, attention was on reducing maternal and infant mortality due to pregnancy and delivery complications. The aim of this study was to interrogate the impact of lockdown measures on women and children in two contrasting districts in Ghana – Krobo Odumase and Ayawaso West Wuogon.
Methods This study adopted the mixed-method approach using both qualitative and quantitative data. The qualitative study relied on two data collection methods to explore the impacts of COVID-19 control measures on women and children in Ghana. These were: Focus Group Discussions (FGDs; n=12) and Key Informant Interviews (KIIs; n = 18).The study complemented the qualitative data with survey data - household surveys (n = 78) and policy data gathered from government websites consisting of government responses to COVID-19.
Results Engagements with participants in the study revealed that the lockdown measures implemented in Ghana had consequences on child and maternal health, and the health care system as a whole. Our study revealed, for example, that there was a decrease in antenatal and postnatal attendance in hospitals. Childhood vaccinations also came to a halt. Obesity and malnutrition were found to be common among children depending on the location of our study participants (urban and rural areas respectively). Our study also revealed that TB, Malaria and HIV treatment seeking reduced due to the fear of going to health facilities since those ailments manifest similar symptoms as COVID 19.
Conclusion Government responded to COVID-19 using different strategies however the policy response resulted in both intended and unintended consequences especially for women and children in Ghana. It is recommended that national policy directions should ensure the continuous provision of child and maternal healthcare services which are essential health services during lockdowns.
Developing and Evaluating a Novel Vessel Ligation Simulator Designed for Teaching Surgical Knot-Tying: A Mixed-Methods Study
Authors: Wu Meng-Yu , Yau Sze Yuen , Lin Jiun-Ren , Lee Li-Ang , Hsieh Ming-Ju 
Published: January 2024

Abstract
Background: Surgical knot-tying is a fundamental learning subject for surgical novices. However, no existing knot-tying training simulator offers a direct objective measurement of knot security, a critical feature of professional performance in this skill. This study aims to bridge this gap by developing a novel knot-tying simulator capable of assessing knot security objectively. Its educational impact was also investigated through professional user evaluation.Methods: A three-phase mixed-methods research study was conducted from August 2021 to September 2022. The first phase involved developing and manufacturing a novel vessel ligation simulator that measured the pressure-holding ability of knots, serving as an indicator of knot security, through post-ligation pressure testing. The second phase involved the participation of expert surgeons (n = 6) and Year-5 medical students (n = 30) in product evaluation. The final phase was a mixed-methods data analysis to determine the educational impact of the simulator. The collected data included participants’ demographics, prior experience in surgical knot-tying, performance metrics on the simulator, experts’ responses to a five-point Likert scale survey of simulator validity, novices’ responses to a 10-point scale survey of self-confidence in mastering surgical knot-tying skills before and after the training course, and their overall perceptions regarding the learning experience associated with the simulator.Results: The simulator was crafted using 3D-printing technology. The experts gave it high scores in face and content validity, with mean scores of 4.5 and 4.77, respectively. Twenty-four novice participants (80%) achieved knot security in vessel ligation by the end of the 3-hour training course, though they required more time for task completion compared to experts. Furthermore, all novice participants reported a marked increase in their post-class self-confidence in skill mastery. The unanimous approval from the novices highlighted the simulator's effectiveness in enhancing their learning, especially its high-fidelity design and objective feedback. This facilitated their reflection and motivation for the post-class deliberate practice.Conclusion: This study not only validates our hypothesis regarding the appropriateness of pressure-holding ability in teaching surgical knot-tying but also establishes a comprehensive methodological framework beneficial for curriculum developers to design effective simulation-based skill training in surgery.
Trust on Personalised Electronic Commerce: A Spanish Case
Authors: Gijón Covadonga , Ruíz-Rúa Aurora , Fernández-Bonilla Fernando , Cuadrado-Gallego Juan-J. , Rodriguez Daniel 
Published: January 2024

Abstract

The privacy and value of users' personal data is closely related to the risk and trust perceived by the users on the Internet about personalised advertising. This paper analyses the level of concern of Internet users in Spain for personalised advertising using datasets from surveys carried out by the Spanish National Institute of Statistics in the years 2020 and 2023. These datasets are composed of socio-demographic variables as well as other variables such as Internet security tools. It could be seen in the results that the most important variables that influence the level of concern with advertising and personalised e-commerce are those related to their personal condition, such as gender, online education, marital and employment status. Also, security features facilitate the use of the Internet when risk perceived by individuals is high and have a greater influence on those individuals who have a higher perception of risk. Finally, the pandemic has increased the concern of individuals regarding the processing of their personal data and personalised publicity. Furthermore, there is an inverse relationship between trust on the Internet and the development of excessive concern about receiving personalised advertising, the positive impact of having suffered from fraud and having high levels of concern about the use of personal data. The importance of the state regarding legal frameworks and the education and knowledge of users in relation to these issues is also highlight.
Upper endoscopy in elderly patients: a multicentre study.
Authors: Zullo Angelo , De Francesco Vincenzo , Amato Arnaldo , Bergna Irene , Bendia Emanuele , Giorgini Giorgia , Buscarini Elisabetta , Manfredi Guido , Cadoni Sergio , Cannizzaro Renato , Realdon Stefano , Ciuffi Mario , Ignomirelli Orazio , Carrara Paola Da Massa , Finucci Giovanni , Somma Antonietta Di , Frandina Chiara , Loria Mariafrancesca , Galeazzi Francesca , Ferrara Francesco , Gemme Carlo , Bertetti Noemi Sara , Gentili Federica , Lotito Antonio , Germanà Bastianello , Russo Nunzia , Grande Giuseppe , Conigliaro Rita , Cravero Federico , Venezia Giovanna , Marmo Riccardo , Senneca Piera , Milano Angelo , Efthymakis Konstantinos , Monica Fabio , Montalto Paolo , Lombardi Mario , Morelli Olivia , Castellani Danilo , Nigro Daniela , Festa Roberto , Peralta Sergio , Grasso Maria , Privitera Antonello , Stefano Maria Emanuela Di , Scaccianoce Giuseppe , Loiacono Mariangela , Segato Sergio , Balzarini Marco , Satta Paolo Usai , Lai Mariantonia , Fortunato Francesca , Manta Raffaele 
Published: January 2024

Abstract

Background: Both macroscopic and histological lesions are frequently detected at upper endoscopy in elderly patients. We assessed the prevalence of the main endoscopic and histological alteration in aged patients who underwent upper endoscopy.
Methods: In this cross-sectional study, clinical, endoscopic, and histological features of consecutive elderly patients referred for upper endoscopy in clinical practice were retrieved. Data were analysed at both univariate and multivariate analyses.
Results: A total of 1,336 (M/F: 595/741; Mean age: 74.1; range: 65-102) underwent upper endoscopy during one month in the 28 participating centres. At endoscopy, at least one macroscopic lesion was present in overall 420 (31.4%) patients. Erosive gastritis (13.3%) and erosive oesophagitis (9.8%) were the most prevalent lesions, whilst Barrett’s oesophagus, gastric ulcer, duodenal ulcer and erosive duodenitis were observed in 1.8%, 2%, 1.4%, and 3.1% patients, respectively. Nine (0.6%) cases of oesophageal, 25 (1.8%) gastric, and 2 (0.1%) duodenal neoplasia were detected. At histology, H. pylori infection was diagnosed in 99 (15.9%) patients, and extensive (antral plus corporal) precancerous lesions on gastric mucosa were detected in 80 patients (14.5%). Endoscopic lesions were more frequent in males, in patients who underwent first endoscopy, and in those with alarm symptoms, whilst were reduced in patients receiving PPI therapy. At multivariate analysis, no independent predictor was found.
Conclusions: Our data found that frequency of erosive and neoplastic lesions remained high in elderly patients, whilst the prevalence of both H. pylori infection and peptic ulcer was decreased.
Evaluation of mining landscape changes with development landscape metrics in the Ostrava-Karviná Mining District (Czech Republic)
Authors: Popelková Renata , Mulková Monika 
Published: January 2024

Abstract

The study presents an analysis of changes in the landscape of the Ostrava-Karviná Mining District (in the Czech Republic) covering the period of more than 170 years. In the area of interest affected by underground coal mining, both areas affected by changes and land cover preserving areas were identified in the study. A detailed assessment of the landscape changes was enabled by using landscape metrics and indices, namely development index and total landscape change index. The underlying data were obtained from maps of stable cadastre (from the year 1836) and aerial images of the years 1947, 1971, and 2009. Visual photointerpretation of aerial images and vectorisation of the maps of stable cadastre made it possible to create land cover maps according to CORINE Land Cover categories. Obtained information on the representation of individual land cover categories were used to identify and to analyse changes in the landscape affected by hard coal mining.
Unveiling phenylpropanoid regulation: the role of DzMYB activator and repressor in durian (Durio zibethinus) fruit
Authors: Weerawanich Kamonwan , Sirikantaramas Supaart 
Published: January 2024

Abstract

The durian fruit has high nutritional value attributed to enriched bioactive compounds, including phenolics, carotenoids, and vitamins. While various transcription factors (TFs) govern phenylpropanoid biosynthesis, MYB TFs emerge as pivotal players in regulating key genes within this pathway. This study delves into the identification of additional candidate MYB TFs from the transcriptome database of the Monthong cultivar at five developmental/postharvest ripening stages. Candidate transcriptional activators were discerned among MYBs upregulated during the ripe stage, based on the positive correlation observed between flavonoid biosynthetic genes and flavonoid contents in ripe durian pulps. Conversely, MYBs downregulated during the ripe stage were considered candidate repressors. The study focused on a candidate MYB activator (DzMYB2) and a candidate MYB repressor (DzMYB3) for functional characterization. LC–MS/MS analysis in Nicotiana benthamiana leaves transiently expressing DzMYB2 revealed increased phenolic compound contents compared to those expressing green fluorescence protein controls, while transiently expressed DzMYB3 led to a decrease in phenolic compounds. Furthermore, it was demonstrated that DzMYB2 controls phenylpropanoid biosynthesis in durian by regulating the promoters of various biosynthetic genes, including phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR). Simultaneously, DzMYB3 regulates the promoters of PAL, 4-coumaroyl-CoA ligase (4CL), CHS, and CHI, resulting in the activation and repression of gene expression, respectively. Additionally, it was discovered that DzMYB2 and DzMYB3 could bind to another TF, DzbHLH1, in the regulation of flavonoid biosynthesis. These findings enhance our understanding of the pivotal role of MYB proteins in regulating the phenylpropanoid pathway in durian pulps.
Identification and expression analysis of Jr4CLs gene family based on transcriptome and physiological data in Walnut ( Juglans regia )
Authors: Ma xiaolan , Gao Yanlong , Zhang zhongxing , Wang xiaoya , Wang Yan xiu 
Published: January 2024

Abstract
Shoot shriveling severely threat growth and development of deciduous trees in northern hemisphere, its essence is imbalance of water absorption and evaporation in the branches. In this study, the physiological characteristics of 'Xiangling' and 'Liaohe (Liaoning No. 4)' during the overwintering process were studied, and key overwintering periods were selected for transcripome analysis. The results showed that plant hormone metabolism, wax metabolism and lignin metabolism were significantly enriched during the overwintering process. Combined with Jr4CL family analysis, the high-expression gene (Jr4CL44) under drought stress was selected by real-time fluorescence quantitative screening for functional verification. Overexpression of Jr4CL44 can effectively remove the reactive oxygen species produced by drought stress, increase lignin content and up-regulate the expression of related genes to improve the drought resistance of Arabidopsis thaliana. These results indicate that Jr4CL44 plays an important role in plant resistance to drought stress, which laies a foundation for further study on the functions in practice.