bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-aromatizable androgens modulate the lipopolysaccharide induced expression of the P2X7 receptor in human adipocytes
Authors: Di Vincenzo Angelo , Granzotto Marnie , Crescenzi Marika , Vettor Roberto , Rossato Marco 
Published: October 2023

Introduction: The activation of the P2X7 receptor subtype (P2X7R) has a main role in orchestrating the cellular inflammatory response in many different tissues. Obesity is characterized by dysfunctional fat deposition leading to a tissue-specific and systemic low-grade inflammation. Androgens and estrogens contribute to the whole adipose tissue inflammatory state, but the involvement of sex steroids in the purinergic signaling modulation in adipocytes is still unknown.Methods: We performed an in vitro study to evaluate the possible role of sex hormones on the P2X7R gene expression in human adipocytes, at baseline and after stimulation with bacterial lipopolysaccharide (LPS). We evaluated P2X7R gene expression during in vitro differentiation of human adipocytes, in the absence and presence of testosterone (T) and 17β-estradiol (E2) in the presence and absence of LPS. Furthermore, we analyzed the effects of incubation with dihydrotestosterone (DHT), a non-aromatizable androgen, using the co-incubation of isolated human adipocytes with T alone or in combination with anastrozole, an inhibitor of aromatase, the enzyme responsible of T conversion to E2.Results: At baseline, incubation of adipocytes with T or E2 did not significantly affect P2X7R gene expression. On the contrary, the incubation with DHT was associated with a significant reduction of P2X7R gene expression. LPS incubation significantly increased gene expression of P2X7R with respect to baseline. Interestingly, after LPS stimulation, DHT exposure showed an additional effect, markedly increasing the P2X7R gene expression. This amplificatory effect was confirmed by the incubation of adipocytes to both anastrozole and testosterone. In these experimental conditions, while no effect was observed at baseline, an amplification of the expression of the P2X7R mRNA was observed after stimulation with LPS.Discussion: The purinergic system is involved in the inflammatory response of adipocytes, and androgens may modulate its activity. In particular DHT, a non-aromatizable androgen, amplifies the LPS-induced P2X7R gene expression in human adipocytes thus showing a gender regulated response of the expression of this purinergic receptor strongly involved in the inflammatory response in adipose tissue.
The impact of mental health stigma in a young Malaysian lady with recurrent suicidal ideations and moribund presentations to the Emergency Department: a case report
Authors: Petrus Chiara Francine , Mohd Salleh Sahimi Hajar , Midin Marhani , Lim Jane Tze Yn 
Published: October 2023

IntroductionMental health stigma (MHS) has been a pervasive social issue and a significant barrier to treatment seeking behavior. The treatment pathways and outcomes for people with mental illness, specifically those with recurrent suicidal ideations and attempts have been influenced by how MHS was experienced in clinical practice.MethodsWe reported a case of a young lady diagnosed with bipolar II disorder, obsessive-compulsive disorder and borderline personality disorder who had recurrent visits to various emergency departments (ED) of tertiary hospitals in Malaysia for suicidality; each time presenting with increased mortality risk and escalating near-lethal outcomes. Among the multiple ED visits after her alleged overdoses of psychotropic medications, thrice she was near-unconscious and had to be intubated for airway protection, subsequently requiring ventilatory support and ICU care. These near-lethal presentations in ED were due to her delays in seeking treatment for fear of re-experiencing the stigmatizing environment among healthcare staff and professionals in the ED.DiscussionThe impact of MHS is detrimental. Effective interventions at various levels in the clinical setting is of utmost importance to prevent the negative consequences of suicidality against MHS.
The pleiotropic contribution of genes in dopaminergic and serotonergic pathways to addiction and related behavioral traits
Authors: Antón-Galindo Ester , Cabana-Domínguez Judit , Torrico Bàrbara , Corominas Roser , Cormand Bru , Fernàndez-Castillo Noèlia 
Published: October 2023

IntroductionCo-occurrence of substance use disorders (SUD) and other behavioral conditions, such as stress-related, aggressive or risk-taking behaviors, in the same individual has been frequently described. As dopamine (DA) and serotonin (5-HT) have been previously identified as key neurotransmitters for some of these phenotypes, we explored the genetic contribution of these pathways to SUD and these comorbid phenotypes in order to better understand the genetic relationship between them.MethodsWe tested the association of 275 dopaminergic genes and 176 serotonergic genes with these phenotypes by performing gene-based, gene-set and transcriptome-wide association studies in 11 genome-wide association studies (GWAS) datasets on SUD and related behaviors.ResultsAt the gene-wide level, 68 DA and 27 5-HT genes were found to be associated with at least one GWAS on SUD or related behavior. Among them, six genes had a pleiotropic effect, being associated with at least three phenotypes: ADH1C, ARNTL, CHRNA3, HPRT1, HTR1B and DRD2. Additionally, we found nominal associations between the DA gene sets and SUD, opioid use disorder, antisocial behavior, irritability and neuroticism, and between the 5-HT-core gene set and neuroticism. Predicted gene expression correlates in brain were also found for 19 DA or 5-HT genes.DiscussionOur study shows a pleiotropic contribution of dopaminergic and serotonergic genes to addiction and related behaviors such as anxiety, irritability, neuroticism and risk-taking behavior, highlighting a role for DA genes, which could explain, in part, the co-occurrence of these phenotypes.
Effect of a school-linked life skills intervention on adolescents' sexual and reproductive health skills in Guji zone, Ethiopia (CRT)—A generalized linear model
Authors: Godana Gobena , Garoma Silesh , Ayers Nicola , Abera Muluembet 
Published: October 2023

BackgroundAlthough appropriate life skills are recognized as a fundamental right for adolescents and a critical component of health policy, they are often overlooked and rarely researched in pastoral communities. Life skills encompass the ability to adopt positive behaviors, which enable individuals to effectively deal with the demands and challenges of everyday life. This study aimed to evaluate the effectiveness of a school-linked life skills intervention program on adolescents' sexual and reproductive health skills in the pastoral community of Guji zone.MethodsA two-arm cluster randomized control trial with a pretest-posttest experimental design was conducted, involving the intervention group (N = 375) and the control group (N = 384). This study assessed the effect of a school-linked adolescent-friendly life skills intervention in comparison to the usual RH curriculum, used as a control arm. Pretest-posttest and posttest-posttest scores of the control group and trial groups were compared, and the data were collected using 27 self-administered questions. The collected data were analyzed using paired-sample independent t-tests and a generalized linear model to examine the relationship between the dependent and independent variables.ResultsData were collected from 759 adolescents in 15 intervention and 15 control clusters. The findings have shown that the proportion of mean life skills score was significantly higher in the intervention clusters than controls [(375) 70.49% vs. (384) 62.25%, P &lt; 0.001 95% CI (0.06 and 0.1)]. Adolescents who were trained in school-linked life skills (β = 1.915, 95% CI: 1.411–2.418), were confident to make safe and informed decisions (β = 1.999, 95% CI: 1.562–2.436), and had life skills to deal with SRH issues (β = 1.66, 95% CI: 1.233–2.087) were significantly correlated with predicting the relevant life skills. The proportion of adolescents with SRH life skills increased from 384 (52%) at baseline to 375 (70%) at end line in the intervention group compared to 384 (60.31%) at baseline to 384 (62.31%) in control arms, respectively.ConclusionsThe implementation of a school-linked life skills intervention program proved to have a significant effect on SRH life skills development. Furthermore, individual-level and behavioral-level variables were significant in explaining variability in life skills development within the pastoral community. Therefore, we recommend scaling up this intervention in all high schools.Trial registrationTrial registration PACTR202107905622610, registered on 16 July 2021.
Factors affecting the willingness of patients with type 2 diabetes to use digital disease management applications: a cross-sectional study
Authors: Zhang Mingjiao , Zhang Hao , Zhu Rong , Yang Huiqi , Chen Mengjie , Wang Xiaoxia , Li Zhe , Xiong Zhenzhen 
Published: October 2023

BackgroundThe global burden of type 2 diabetes has significantly increased, leading to a considerable impact on healthcare systems worldwide. While the advent of mobile healthcare has provided some relief by addressing the shortage of certain medical resources, its adoption among the Chinese population remains relatively low. To extend the benefits of mHealth to a greater number of Chinese diabetic patients, it is essential to investigate the factors that influence their willingness to utilize it and implement targeted interventions based on these influencing factors. The Technology Acceptance Model (TAM) is widely employed to examine users' ultimate usage behaviors, and previous studies have indicated the potential relevance of the Perceived Risk (PR) theory and the eHealth Literacy Theory to users' usage behaviors.ObjectiveOur objective was to investigate the determinants that affect the willingness of Chinese patients diagnosed with type 2 diabetes patients to utilize digital disease management applications (DDMAs).MethodsWe conducted a cross-sectional study of patients with type 2 diabetes in three tertiary general hospitals in Chengdu using questionnaires designed by the investigators. Participants were sampled using a convenience sampling method. The questionnaire comprised three sections: socio-demographic profile and medical history; current awareness and willingness to use digital disease management applications; and the current level of e-health literacy. Structural equation modeling was employed to assess the impact of patient awareness of DDMAs and e-health literacy on the willingness to use such DDMAs.Results(1) Patients' attitudes toward using DDMAs were significantly influenced by perceived ease of use (β = 0.380, P &lt; 0.001) and perceived usefulness (β = 0.546, P &lt; 0.001); (2) Electronic health literacy exerted a significant impact on patients' perceived usefulness (β = 0.115, P = 0.018) and perceived ease of use (β = 0.659, P &lt; 0.001); (3) Patients' willingness to use was significantly influenced by perceived usefulness (β = 0.137, P &lt; 0.001) and use attitude (β = 0.825, P &lt; 0.001).ConclusionsThe present research findings hold both theoretical and practical significance, and can serve as a guide for healthcare practitioners and researchers to gain a deeper comprehension of the acceptance of digital disease management applications (DDMAs) among type 2 diabetes patients.
Assessing the pre-implementation context for financial navigation in rural and non-rural oncology clinics
Authors: Petermann Victoria M. , Biddell Caitlin B. , Planey Arrianna Marie , Spees Lisa P. , Rosenstein Donald L. , Manning Michelle , Gellin Mindy , Padilla Neda , Samuel-Ryals Cleo A. , Birken Sarah A. , Reeder-Hayes Katherine , Deal Allison M. , Cabarrus Kendrel , Bell Ronny A. , Strom Carla , Young Tiffany H. , King Sherry , Leutner Brian , Vestal Derek , Wheeler Stephanie B. 
Published: October 2023

BackgroundFinancial navigation (FN) is an evidence-based intervention designed to address financial toxicity for cancer patients. FN's success depends on organizations' readiness to implement and other factors that may hinder or support implementation. Tailored implementation strategies can support practice change but must be matched to the implementation context. We assessed perceptions of readiness and perceived barriers and facilitators to successful implementation among staff at nine cancer care organizations (5 rural, 4 non-rural) recruited to participate in the scale-up of a FN intervention. To understand differences in the pre-implementation context and inform modifications to implementation strategies, we compared findings between rural and non-rural organizations.MethodsWe conducted surveys (n = 78) and in-depth interviews (n = 73) with staff at each organization. We assessed perceptions of readiness using the Organizational Readiness for Implementing Change (ORIC) scale. In-depth interviews elicited perceived barriers and facilitators to implementing FN in each context. We used descriptive statistics to analyze ORIC results and deductive thematic analysis, employing a codebook guided by the Consolidated Framework for Implementation Research (CFIR), to synthesize themes in barriers and facilitators across sites, and by rurality.ResultsResults from the ORIC scale indicated strong perceptions of organizational readiness across all sites. Staff from rural areas reported greater confidence in their ability to manage the politics of change (87% rural, 76% non-rural) and in their organization's ability to support staff adjusting to the change (96% rural, 75% non-rural). Staff at both rural and non-rural sites highlighted factors reflective of the Intervention Characteristics (relative advantage) and Implementation Climate (compatibility and tension for change) domains as facilitators. Although few barriers to implementation were reported, differences arose between rural and non-rural sites in these perceived barriers, with non-rural staff more often raising concerns about resistance to change and compatibility with existing work processes and rural staff more often raising concerns about competing time demands and limited resources.ConclusionsStaff across both rural and non-rural settings identified few, but different, barriers to implementing a novel FN intervention that they perceived as important and responsive to patients' needs. These findings can inform how strategies are tailored to support FN in diverse oncology practices.
Responses of the wintertime auroral E-region neutral wind to varying levels of geomagnetic activity
Authors: Zhan Weijia , Keappler Stephen R. 
Published: October 2023

Introduction: The auroral E-region is an important interface where forces from the magnetosphere and the lower atmosphere converge and have a significant effect on the vertical structure of the neutral winds. The resulting vertical neutral wind structure has been reported to be associated with altitude-dependent and nonlinear effects from different forces. We conduct a statistical examination of the reactions of wintertime neutral winds to four various degrees of geomagnetic activity, with a focus on the impacts of ion drag.Methods: We derive neutral winds using the PFISR measurements covering 2010–2019 and will give a statistical view of the auroral E-region neutral wind with a focus on the winter nighttime during different disturbed conditions. We investigate the effects of the geomagnetic activity on the neutral winds and the tidal components by dividing the dataset into 4 subsets. Tidal decomposition is conducted by least square fitting of the seasonal median winds to obtain the mean, diurnal amplitude, diurnal phase, semidiurnal amplitude, and semidiurnal phase.Results and discussion: We find that 1) when geomagnetic activity increases, dawn-dusk asymmetry exists in both zonal and meridional winds in the upper E-region with stronger zonal wind in the dusk sector than in the dawn sector and much stronger meridional wind in the dawn sector than in the dusk sector. 2) Tidal decomposition results reveal that geomagnetic activity has more significant effects on the meridional diurnal amplitude than zonal diurnal amplitude while the zonal and meridional semidiurnal amplitudes show similar changes when the geomagnetic activity increases. In addition, the maximum semidiurnal amplitude, particularly in the zonal direction, appears at a higher altitude with larger values as geomagnetic activity increases, indicating an ascending transition altitude for the semidiurnal oscillations. The ascending trend of maximum semidiurnal amplitude appearing at higher altitudes during more disturbed conditions has not been reported before. 3) Zonal wind over 110 km demonstrates increasing ion drag effects in the evening sector and the effects of coupled ion drag and other factors after midnight.
Liver microbial community and associated host transcriptome in calves with feed induced acidosis
Authors: Li Wenli , Larsen Anna , Murphy Brianna , Fregulia Priscila 
Published: October 2023

IntroductionIn the dairy industry, calves are typically fed diets rich in highly fermentable carbohydrates and low in fibrous feeds to maximize ruminal papillae and tissue development. Calves on such diets are vulnerable at developing ruminal acidosis. Prevalent in cattle, liver abscess (LA) is considered a sequela to ruminal acidosis. LAs can cause significant liver function condemnation and decreased growth and production. Currently, we know little about the liver microbiome in calves with feed-induced acidosis.MethodsUsing our established model of ruminal acidosis, where young calves were fed an acidosis-inducing (AC) or -blunting (control) diet starting at birth until 17-week of age, we investigated microbial community changes in the liver resultant from ruminal acidosis. Eight calves were randomly assigned to each diet, with four animals per treatment. Rumen epithelium and liver tissues were collected at 17 weeks of age right after euthanasia. Total RNAs were extracted and followed by whole transcriptome sequencing. Microbial RNA reads were enriched bioinformatically and used for microbial taxonomy classification using Kraken2.ResultsAC Calves showed significantly less weight gain over the course of the experiment, in addition to significantly lower ruminal pH, and rumen degradation comparison to the control group (p&lt; 0.05). In the liver, a total of 29 genera showed a significant (p&lt; 0.05) abundance change (&gt; 2-fold) between the treatments at 17-week of age. Among these, Fibrobacter, Treponema, Lactobacillus, and Olsenella have been reported in abscessed liver in cattle. Concurrent abundance changes in 9 of the genera were observed in both the liver and rumen tissues collected at 17-week of age, indicating potential crosstalk between the liver and rumen epithelial microbial communities. Significant association was identified between host liver gene and its embedded microbial taxa. Aside from identifying previously reported microbial taxa in cattle abscessed liver, new repertoire of actively transcribed microbial taxa was identified in this study.DiscussionBy employing metatranscriptome sequencing, our study painted a picture of liver microbiome in young calves with or without feed induced acidosis. Our study suggested that liver microbiome may have a critical impact on host liver physiology. Novel findings of this study emphasize the need for further in-depth analysis to uncover the functional roles of liver resident microbiome in liver metabolic acidosis resultant from feed-related ruminal acidosis.
Maternal Healthcare Services Utilisation and Its Associated Risk Factors: A Pooled Study of 37 Low- and Middle-Income Countries
Authors: Shanto Hasibul Hasan , Al-Zubayer Md. Akib , Ahammed Benojir , Sarder Md. Alamgir , Keramat Syed Afroz , Hashmi Rubayyat , Haque Rezwanul , Alam Khorshed 
Published: October 2023

Objectives: The utilisation of maternal healthcare services (MHS) can play an essential role in reducing maternal deaths. Thus, this study examines the prevalence and factors associated with MHS utilisation in 37 low-and-middle-income countries (LMICs).Methods: A total of 264,123 women were obtained from the Demographic and Health Surveys of 37 LMICs. Multivariate logistic regression was performed to identify the factors associated with maternal healthcare services utilisation.Results: Around one-third (33.7%) of the respondents properly utilise MHS among women of childbearing age. In the pooled sample, the odds of MHS utilisation were significantly higher with the increase in wealth index, women’s age, age at the first birth, and husband/partner’s education. Urban residence (AOR [adjusted odds ratio] = 1.56; 95% CI [confidence interval]: 1.49–1.64), women’s autonomy in healthcare decision-making (AOR = 1.19; 95% CI: 1.15–1.24) and media exposure (AOR = 1.70; 95% CI: 1.58–1.83) were found to be the strongest positive factors associated with utilisation of MHS. In contrast, larger family (AOR = 0.93; 95% CI: 0.91–0.96), and families with 7 or more children (AOR = 0.72; 95% CI: 0.68–0.77) were significantly negatively associated with MHS utilisation.Conclusion: The utilisation of MHS highly varied in LMICs and the associated factors. Expanding the wealth status, education, age at first birth, mothers’ autonomy in healthcare decisions, and media exposure could be essential strategies for increasing the utilisation of MHS; however, country-specific programs should be considered in national policy discussions. There is a need to formulate policies and design maternal health services programs that target socially marginalised women.
A New Global Air Quality Health Index Based on the WHO Air Quality Guideline Values With Application in Cape Town
Authors: Adebayo-Ojo Temitope Christina , Wichmann Janine , Arowosegbe Oluwaseyi Olalekan , Probst-Hensch Nicole , Schindler Christian , Künzli Nino 
Published: October 2023

Objectives: This study developed an Air Quality Health Index (AQHI) based on global scientific evidence and applied it to data from Cape Town, South Africa.Methods: Effect estimates from two global systematic reviews and meta-analyses were used to derive the excess risk (ER) for PM2.5, PM10, NO2, SO2 and O3. Single pollutant AQHIs were developed and scaled using the ERs at the WHO 2021 long-term Air Quality Guideline (AQG) values to define the upper level of the “low risk” range. An overall daily AQHI was defined as weighted average of the single AQHIs.Results: Between 2006 and 2015, 87% of the days posed “moderate to high risk” to Cape Town’s population, mainly due to PM10 and NO2 levels. The seasonal pattern of air quality shows “high risk” occurring mostly during the colder months of July–September.Conclusion: The AQHI, with its reference to the WHO 2021 long-term AQG provides a global application and can assist countries in communicating risks in relation to their daily air quality.