bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intelligent navigation for the cruise phase of solar system boundary exploration based on Q-learning EKF
Authors: Tao Wenjian , Zhang Jinxiu , Hu Hang , Zhang Juzheng , Sun Huijie , Zeng Zhankui , Song Jianing , Wang Jihe 
Published: December 2023

AbstractWith the continuous advancement of deep space exploration missions, the solar system boundary exploration mission is established as one of the China's most important deep space scientific exploration missions. The mission of the solar system boundary exploration has many challenges such as ultra-remote detection distance, ultra-long operation time, and ultra-long communication delay. Therefore, the problem of high-precision autonomous navigation needs to be solved urgently. This paper designs an autonomous intelligent navigation method based on X-ray pulsars in the cruise phase, which estimate the motion state of the probe in real time. The proposed navigation method employs the Q-learning Extended Kalman filter (QLEKF) to improve navigation accuracy during long periods of self-determining running. The QLEKF selects automatically the error covariance matrix parameter of the process noise and the measurement noise by the reward mechanism of reinforcement learning. Compared to the traditional EKF and AEKF, the QLEKF improves the estimation accuracy of position and velocity. Finally, the simulation result demonstrates the effectiveness and the superiority of the intelligent navigation algorithm based on QLEKF, which can satisfy the high-precision navigation requirements in the cruise phase of the solar system boundary exploration.
A critical review of the interactions between rhizosphere and biochar during the remediation of metal(loid) contaminated soils
Authors: Fan Chuanfang , Cui Yanshan , Zhang Qianru , Yin Naiyi , Cai Xiaolin , Yuan Xiangzhou , Senadheera Sachini , Cho Yoora , Ok Yong Sik 
Published: December 2023

AbstractBiochar has a large specific surface area, well-developed pore structure, abundant surface functional groups, and superior nutrient supply capacity, which is widely available and environmentally friendly with its advantages in waste resource utilization, heavy metal(loid) remediation, and carbon storage. This review focuses on the interactions between biochar (including raw biochar, functional biochar (modified/ engineered/ designer biochar), and composite biochar) and rhizosphere during the remediation of soil contaminated with heavy metal(loid)s (Pb, As, Cd, Hg, Co, Cu, Ni, Zn, Cr, etc.) and the effects of these interactions on the microbial communities and root exudates (enzymes and low-molecular-weight organic acids (LMWOAs)). In terms of microorganisms, biochar affects the composition, diversity, and structure of microbial communities through the supply of nutrients, provision of microbial colonization sites, immobilization of heavy metal(loid)s, and introduction of exogenous microorganisms. With regard to root exudates, biochar provides electron transfer support between the microorganisms and exudates, regulates the secretion of enzymes to resist the oxidative stress stimulated by heavy metal(loid)s, ameliorates rhizosphere acidification caused by LMWOAs, and promotes the activity of soil enzymes. The roles and mechanisms of biochar on rhizosphere soils are discussed, as well as the challenges of biochar in the remediation of heavy metal(loid)-contaminated soils, and the issues that need to be addressed in future research are foreseen.
Graphical Abstract
Community health surveillance via digital collection of syndromic and behavior data by community healthcare workers in rural Kenya: a pilot study
Authors: van Herpen Maarten M. J. W. , Saadah Nicholas H. , Otieno Pieter , Kiara Lemmy , Diehl J. C. 
Published: December 2023

AbstractDisease surveillance is essential to enable adequate detection and response to disease outbreaks. Syndromic surveillance is used to augment traditional approaches, especially in community-based surveillance. Here we demonstrate that Community Healthcare Workers (CHWs) supported by a mobile phone application can provide community-based syndromic disease surveillance in low-resource settings, and that they are able to generate relevant symptom-based and behavior data such as cough symptoms, use of mosquito nets and availability of household handwashing facilities. We analyzed 1.6 million data points collected by CHWs during home visits in rural Kenya as a proof of principle that the symptoms and behavior they observe can be used as a community-based health surveillance tool. To demonstrate the relevance of the data, we show that national covid-19 case numbers did not align with reported cough symptoms in remote populations, which implies that rural populations did not experience covid-19 outbreaks in tandem with urban populations. We also found that the behavior of using long-lasting insecticidal nets could be tracked by the CHWs, and it followed the seasonality of the mosquito burden. Strengthening community-based syndromic and behavior surveillance through CHWs is therefore a great opportunity to strengthen national public health surveillance and response in Africa and should be included in the Integrated Disease Surveillance and Response (IDSR) strategy.
Conceptualization, development, and early dissemination of eMPACTTM: A competency-based career navigation system for translational research professionals
Authors: Choi Ikseon , Kwon Sejung , Rojewski Jay W. , Hill Janette R. , Kim Eunice S. , Fisher Elaine , Thomas Rebecca S. , McCauley Linda 
Published: December 2023

Abstract

Introduction:
Purposeful training and ongoing career support are necessary to meet the evolving and expanding roles of clinical research professionals (CRP). To address the training and employment needs of clinical research coordinators (CRCs), one of the largest sectors of the CRP workforce, we designed, developed, and implemented an online career navigation system, eMPACTTM (eMpowering Purposeful Advancement of Careers and Training).

Methods:
A design-based research method was employed as an overarching approach that frames iterative design, development, and implementation of educational interventions. The five major phases of this project – conceptualization, task analysis for measurement development, algorithms development, algorithms validation, and system evaluation – presented specific goals and relevant methods.

Results:
The results reported how the eMPACTTM system was conceptualized, developed, and validated. The system allowed CRCs to navigate tailored training and job opportunities by completing their task competencies and career goals. The data sets could, in turn, support employees’ and training coordinators’ informed decisions about organizational training needs and recruitment. The early dissemination results showed steady growth in registered CRCs and diversity in users’ ethnicity and job levels.

Conclusions:
The eMPACTTM service showed the possibility of supporting CRCs’ individual career advancement and organizational workforce enhancement and diversity. Long-term research is needed to evaluate its impact on CRC workforce development, explore key factors influencing workforce sustainability, and expand eMPACTTM service to other CRP sectors.
Cycling Assessment: A tool to inform policymakers and enhance the cyclist’s travel experience, with a gender perspective
Authors: Ángel Angie , Gómez Laura Daniela , Rincón Maryfely 
Published: December 2023

Abstract
Since 1998, Bogotá has consistently made substantial efforts to foster the bicycle’s role as a primary mode of transportation. Recent years have witnessed a compelling aspiration for the city to ascend as the “bicycle capital of the world,” evident in its accomplishment of 6.6% of daily trips completed by bicycle in 2019. This achievement translates to 880.367 daily cycling journeys (District Secretariat of Mobility of Bogotá, 2019). These statistics surpass regional benchmarks; for instance, other capital cities such as Santiago de Chile account for 510.569 bicycle trips, Mexico City for 433.981, and Rio de Janeiro for 217.000 (Ríos et al., 2015). Despite this progress, Bogotá lacks a comprehensive evaluation of both infrastructure quality and the user experience while cycling.
This translational research article aimed to explore this gap by delving into the integration of user perceptions and experiences within the policy formulation process. This strategic approach is poised to enhance cycling’s allure as a mode of transportation for prospective cyclists while simultaneously maximizing the efficiency of investments in cycling infrastructure.
The Basel Compactata and the Limits of Religious Coexistence in the Age of Conciliarism and Beyond
Authors: Pálka Adam 
Published: December 2023

AbstractThe Compactata, one of the most significant documents related to the Council of Basel, have not been analyzed and understood properly in the historiography, both in relation to their content and impact. This article aims to provide a better understanding of the Basel Compactata by discussing the controversial nature of these documents as demonstrated in international diplomacy and polemical writings of the fifteenth century. The diplomatic missions of J. Carvajal, N. Cusanus, J. Capistrano, and E. S. Piccolomini prove that the Compactata could easily have become a crucial bone of contention between Catholics and Bohemian Utraquists even on the international level. Rather surprisingly, the Catholic diplomats’ negative approach toward the Compactata does not appear to have been influenced by their origins in the controversial Council of Basel, for other phenomena such as craving for perfect unity and alleged transgressions of the treaties played a more prominent role. A thorough examination of polemical writings shows that there existed major differences between the standard Catholic and Utraquist interpretation of the key provision of the Compactata, which was possible due to their compromise wording. Such differences could affect considerably the situation in the Czech Lands. For instance, the emergence of a semi-independent Utraquist Church after 1436 was not explicitly stated in the Compactata and was enabled by the manner in which the Utraquists interpreted their text. Although the Compactata did contribute to the stabilization of the political situation in the Czech Lands, unproductive disputes over their meaning actually disturbed the idea of peaceful coexistence between the Catholics and Utraquists.
Efficient gene editing in induced pluripotent stem cells enabled by an inducible adenine base editor with tunable expression
Authors: Nandy Krittika , Babu Dinesh , Rani Sonam , Joshi Gaurav , Ijee Smitha , George Anila , Palani Dhavapriya , Premkumar Chitra , Rajesh Praveena , Vijayanand S. , David Ernest , Murugesan Mohankumar , Velayudhan Shaji R. 
Published: December 2023

AbstractThe preferred method for disease modeling using induced pluripotent stem cells (iPSCs) is to generate isogenic cell lines by correcting or introducing pathogenic mutations. Base editing enables the precise installation of point mutations at specific genomic locations without the need for deleterious double-strand breaks used in the CRISPR-Cas9 gene editing methods. We created a bulk population of iPSCs that homogeneously express ABE8e adenine base editor enzyme under a doxycycline-inducible expression system at the AAVS1 safe harbor locus. These cells enabled fast, efficient and inducible gene editing at targeted genomic regions, eliminating the need for single-cell cloning and screening to identify those with homozygous mutations. We could achieve multiplex genomic editing by creating homozygous mutations in very high efficiencies at four independent genomic loci simultaneously in AAVS1-iABE8e iPSCs, which is highly challenging with previously described methods. The inducible ABE8e expression system allows editing of the genes of interest within a specific time window, enabling temporal control of gene editing to study the cell or lineage-specific functions of genes and their molecular pathways. In summary, the inducible ABE8e system provides a fast, efficient and versatile gene-editing tool for disease modeling and functional genomic studies.
Research on cross regional emergency material scheduling algorithm based on seed optimization algorithm
Authors: Li Jinbao , Cui Lichong , Chu Huayu , Su Lei , Wang Junsheng 
Published: December 2023

AbstractIn order to improve the response capability of cross regional emergency material scheduling (CREMS), a CREMS algorithm based on seed optimization algorithm is proposed. Construct a segmented regional grid distribution model structure for CREMS, use a grid matching algorithm based on block link distribution to construct the optimization objective function during the emergency material scheduling process, use variable neighborhood search technology to solve the diversity problem of cluster optimization in CREMS, and combine seed optimization algorithms for combination control and recursive analysis in the emergency material scheduling process. Based on the combination of deep learning and reinforcement learning, the optimal route and configuration scheme design for CREMS process is achieved. The simulation results show that this method has better active configuration capability, better path optimization capability and stronger spatial regional planning capability for CREMS.
Association analysis of Vascular Endothelial Growth Factor-A (VEGF-A) polymorphism in rheumatoid arthritis using computational approaches
Authors: Ahmed Iraj , John Peter , Bhatti Attya 
Published: December 2023

AbstractRheumatoid arthritis (RA), is marked by joint inflammation leading to pannus formation which results in cartilage destruction promoting bone erosion. The pathological hallmark of RA includes synovial hyperplasia and synovial angiogenesis. Active tissue neovascularization is observed in RA. Vascular endothelial Growth factor A (VEGFA), an endothelial cell-specific proangiogenic molecule is triggered by hypoxic cells and its levels are upregulated in RA. The aim of this study was to investigate functional and pathogenic VEGFA variants and to identify the impact of point mutation in VEGFA’s interaction with VEGFR2 and how these polymorphisms affect the susceptibility and severity of RA. We investigated impact of these point mutations on the stability of VEGFA using various computational tools. These mutations were further identified by conservational profile as they are highly involved as structural and functional mutations. Furthermore, these selected variants were modelled and docked against targeted domain regions IGD2 and IGD3 of VEGFR2. Further molecular dynamic simulations were performed using Gromacs. Out of 168 nsSNPS, 19 were highlighted as highly pathogenic using insilico prediction tools. InterPro and ConSurf revealed domains and conserved variants respectively. After stability analysis, we concluded that almost all the mutations were responsible for decreasing the protein stability. HOPE predicted that all the selected damaging nsSNPs were present in the domain which is essential for the functioning of VEGFA protein. Constructed Ramachandran plot and ERRAT validated the quality of all the models. Based on the interactions predicted by STRING database, we performed Protein–Protein docking between VEGFA and VEGFR2. We found few conserved interactions and new polar contacts among wild-type and mutants with VEGFR2. From the simulations, we concluded that mutant R108Q was the most stabilizing mutant among all others whereas R82Q, C86Y, and R108W complexed with VEGFR2 were comparatively less stabilizing as compared to the wild type. This study provides insight into pathogenic nsSNPs that can affect VEGFA protein structure and function. These high-risk variants must be taken into consideration for genetic screening of patients suffering from RA.
Molecular mechanism of CCDC106 regulating the p53-Mdm2/MdmX signaling axis
Authors: Zhou Ting , Ke Zhiqiang , Ma Qianqian , Xiang Jiani , Gao Meng , Huang Yongqi , Cheng Xiyao , Su Zhengding 
Published: December 2023

AbstractThe tumor suppressor p53 (p53) is regulated by murine double minute 2 (Mdm2) and its homologous MdmX in maintaining the basal level of p53. Overexpressed Mdm2/MdmX inhibits cellular p53 activity, which is highly relevant to cancer occurrence. Coiled-coil domain-containing protein 106 (CCDC106) has been identified as a p53-interacting partner. However, the molecular mechanism of the p53/Mdm2/MdmX/CCDC106 interactions is still elusive. Here, we show that CCDC106 functions as a signaling regulator of the p53-Mdm2/MdmX axis. We identified that CCDC106 directly interacts with the p53 transactivation domain by competing with Mdm2 and MdmX. CCDC106 overexpression downregulates the cellular level of p53 and Mdm2/MdmX, and decreased p53 reversibly downregulates the cellular level of CCDC106. Our work provides a molecular mechanism by which CCDC106 regulates the cellular levels of p53 and Mdm2/MdmX.
Dopamine precursor depletion affects performance and confidence judgements when events are timed from an explicit, but not an implicit onset
Authors: Jovanovic Ljubica , Chassignolle Morgane , Schmidt-Mutter Catherine , Behr Guillaume , Coull Jennifer T. , Giersch Anne 
Published: December 2023

AbstractDopamine affects processing of temporal information, but most previous work has tested its role in prospective tasks, where participants know in advance when the event to be timed starts. However, we are often exposed to events whose onset we do not know in advance. We can evaluate their duration after they have elapsed, but mechanisms underlying this ability are still elusive. Here we contrasted effects of acute phenylalanine and tyrosine depletion (APTD) on both forms of timing in healthy volunteers, in a within-subject, placebo-controlled design. Participants were presented with a disc moving around a circular path and asked to reproduce the duration of one full revolution and to judge their confidence in performance. The onset of the revolution was either known in advance (explicit onset) or revealed only at the end of the trial (implicit onset). We found that APTD shortened reproduced durations in the explicit onset task but had no effect on temporal performance in the implicit onset task. This dissociation is corroborated by effects of APTD on confidence judgements in the explicit task only. Our findings suggest that dopamine has a specific role in prospective encoding of temporal intervals, rather than the processing of temporal information in general.
An expanded RT-PCR melting temperature coding assay to rapidly identify all known SARS-CoV-2 variants and sub-variants of concern
Authors: Banada Padmapriya P. , Green Raquel , Streck Deanna , Kurvathi Rohini , Reiss Robert , Banik Sukalyani , Daivaa Naranjargal , Montalvan Ibsen , Jones Robert , Marras Salvatore A. E. , Chakravorty Soumitesh , Alland David 
Published: December 2023

AbstractThe continued emergence of vaccine-resistant SARS-CoV-2 variants of concern (VOC) requires specific identification of each VOC as it arises. Here, we report an expanded version of our previously described sloppy molecular beacon (SMB) melting temperature (Tm) signature-based assay for VOCs, now modified to include detection of Delta (B.1.617.2) and Omicron (B.1.1.529) sub-variants. The SMB-VOC assay targets the signature codons 501, 484 and 452 in the SARS-CoV-2 spike protein which we show can specifically detect and differentiate all known VOCs including the Omicron subvariants (BA.1, BA.2, BA.2.12.1, BA.4/BA.5). The limit of detection (LOD) of the assay was 20, 22 and 36 genomic equivalents (GE) per reaction with the Delta, Omicron BA.1 and BA.2 respectively. Clinical validation of the 3-codon assay in the LC480 instrument showed the assay detected 94% (81/86) of the specimens as WT or VOCs and 6% (5/86) of the tests producing indeterminate results compared to sequencing. Sanger sequencing also failed for four samples. None of the specimens were incorrectly identified as WT or as a different VOC by our assay. Thus, excluding specimens with indeterminant results, the assay was 100% sensitive and 100% specific compared to Sanger sequencing for variant identification. This new assay concept can be easily expanded to add newer variants and can serve as a robust diagnostic tool for selecting appropriate monoclonal antibody therapy and rapid VOC surveillance.
Multiphase CT angiography perfusion maps for predicting target mismatch and ischemic lesion volumes
Authors: Chung Kevin J. , Pandey Sachin K. , Khaw Alexander V. , Lee Ting-Yim 
Published: December 2023

AbstractThe complexity of CT perfusion (CTP) acquisition protocols may limit the availability of target mismatch assessment at resource-limited hospitals. We compared CTP mismatch with a mismatch surrogate generated from a simplified dynamic imaging sequence comprising widely available non-contrast CT (NCCT) and multiphase CT angiography (mCTA). Consecutive patients with anterior circulation acute ischemic stroke who received NCCT, mCTA, and CTP were retrospectively included in this study. An mCTA-perfusion (mCTA-P) dynamic series was formed by co-registering NCCT and mCTA. We simulated an ideal mCTA-P study by down-sampling CTP (dCTP) dynamic images according to mCTA timing. Ischemic core and penumbra volumes were estimated by cerebral blood flow and Tmax thresholding, respectively, on perfusion maps calculated independently for CTP, dCTP, and mCTA-P by deconvolution. Concordance in target mismatch (core < 70 ml, penumbra ≥ 15 ml, mismatch ratio ≥ 1.8) determination by dCTP and mCTA-P versus CTP was assessed. Of sixty-one included patients, forty-six had a CTP target mismatch. Concordance with CTP profiles was 90% and 82% for dCTP and mCTA-P, respectively. Lower mCTA-P concordance was likely from differences in collimation width between NCCT and mCTA, which worsened perfusion map quality due to a CT number shift at mCTA. Moderate diagnostic agreement between CTP and mCTA-P was found and may improve with optimal mCTA scan parameter selection as simulated by dCTP. mCTA-P may be a pragmatic alternative where CTP is unavailable or the risks of additional radiation dose, contrast injections, and treatment delays outweigh the potential benefit of a separate CTP scan.