bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low Cost Co3O4 &amp; Neem Leaves Derived Carbon Composite: High-Performance Oxygen Reduction Electro-catalyst for AEMFC
Authors: Nagane Dhiraj , NILEGAVE DHANARAJ S. , Girawale Swapnil , Patil Sachin L. , Shaikh Gulistan Y. , Jadhav Vijay , Pathak Anagha 
Published: February 2024

Abstract
The catalyst development for the oxygen reduction reaction (ORR) which is an important part of metal-air batteries and fuel cells, is immensely profitable. Along with the aim of development, cost-effectiveness is a needful parameter that gives competitive aspects of the product in the market of energy storage and conversion field. Here, we report the co-precipitation synthesis of cost-effective Co3O4 nanoparticles with the neem leaves-derived carbon (NC) as an efficient oxygen reduction catalyst resulting in a scalable alternative. The structural, phase analysis, thermo-gravimetric, and electrochemical analysis are studied by imposing the pertinent experimental techniques of the prepared catalyst Co3O4@NC. Moreover, the affirmation of the pure phase Co3O4@NC has been done by the oxidation state analysis by X-day photoelectron microscopy. Further, the performance of the fuel cell was tested with distinct loading of catalyst in which for equal loadings of standard platinum-based Ptc 40% and with replacing with Co3O4@NC. The open circuit potential of the Ptc 40 wt% based cell supply is 0.957 and for Co3O4@NC is 0.925 which is just 3.34% less than Ptc 40%. The low cost and highly active oxygen reduction reaction (ORR) cathode Co3O4@NC has been demonstrated in anion-exchange membrane fuel cell (AEMFC) with single-cell assembly.
Facile Layer Diffusion Technique for Synthesis of Terbium-Based Metal Organic Framework for Fluorometric Sensing of Hydroquinone
Authors: Singh Gurdeep , Verma Rajpal , Kaur Kirandeep , - Deepika , Kumar Sanjay , Malik Ashok Kumar 
Published: February 2024

Abstract
A photoluminescent terbium(III)-based Metal Organic Framework (MOF) was synthesized at room temperature by layer diffusion method utilising mixed carboxylate linkers (4,4'-oxybis(benzoic acid) and benzene-1,3,5 tricarboxylic acid). Synthesized MOF has crystalline nature and rod-shaped morphology and is thermally stable up to 455°C. From elemental analysis, Carbon, oxygen, and terbium were all found to be present in the synthesized MOF. The fluorescence emission spectra and theoretical results revealed that carboxylate linkers functioned as sensitizers for Tb(III) photoluminescence which resulted in four distinct emission peaks at 495, 547, 584, and 621 nm corresponding to the transitions 5D4→7F6, 5D4→7F5, 5D4→7F4, and 5D4→7F3. Using synthesized MOF as fluorescent probe, hydroquinone was detected in aqueous medium with a detection limit of 0.048 μM, remarkable recovery (95.6-101.1%), and relative standard deviation less than 2.25%. The quenching phenomenon may be ascribed by electron transfer from synthesized probe to oxidized hydroquinone via carboxylic groups on the surface of MOF, which is further supported by photo-induced electron transfer mechanism. This study introduces a cheaper, faster, and more accurate method for hydroquinone detection.
Adrenergic Reprogramming of Preexisting Adipogenic Trajectories Steer Naïve Mural Cells Toward Beige Differentiation
Authors: Rabhi Nabil , Desevin Kathleen , Cortez Briana , Lin Jean , Lama Dechen , Layne Matthew , Farmer Stephen 
Published: February 2024

Abstract
In adult white adipose tissue, cold or β3-adrenoceptor activation promotes the appearance of thermogenic beige adipocytes. Our comprehensive single-cell analysis revealed that these cells arise through the reprogramming of existing adipogenic trajectories, rather than from a single precursor. These trajectories predominantly arise from SM22-expressing vascular mural progenitor cells. Central in this transition is the activation of Adrb3 in mature adipocytes, leading to subsequent upregulation of Adrb1 in primed progenitors. Under thermoneutral conditions, synergistic activation of both Adrb3 and Adrb1 recapitulates the pattern of cold-induced SM22+ cell recruitment. Lipolysis-derived eicosanoids, specifically docosahexaenoic acid (DHA) and arachidonic acid (AA) prime these processes and in vitro, were sufficient to recapitulate progenitor cells priming. Collectively, our findings provide a robust model for cold-induced beige adipogenesis, emphasizing a profound relationship between mature adipocytes and mural cells during cold acclimation, and revealing the metabolic potential of this unique cellular reservoir.
The relationship between zinc and epilepsy
Authors: Luo Dadong , Liu Yaqing , Li Junqiang , Liu Xuhui , Liu Xuejuan , Zhang Ruirui , Zhang Wenzhao , Liu Jiayi , Wang Tiancheng 
Published: February 2024

Abstract
Background
Previous studies have indicated a potential relationship between zinc and epilepsy. The aim of this study is to investigate the causal relationship between zinc, zinc-dependent carbonic anhydrase, and gray matter volume in brain regions enriched with zinc, in relation to epileptic seizures, as well as explore the possible mechanisms by which zinc contributes to epilepsy.
Methods
First, this study assessed the risk causality between zinc, carbonic anhydrase, and gray matter volume alterations in zinc-enriched brain regions and various subtypes of epilepsy based on two-sample Mendelian randomization analysis. And then, Then, this study conducted GO/KEGG analysis based on colocalization analysis, MAGMA analysis, lasso regression, random forest model and xgboot model.
Results
1. There was a causal relationship between zinc, carbonic anhydrase-4, and generalized epilepsy (p = 0.044, p = 0.010). Additionally, carbonic anhydrase-1 and gray matter volume of the caudate nucleus were found to be associated with epilepsy and focal epilepsy (p = 0.014, p = 0.003, p = 0.022, p = 0.009).2. A colocalization relationship was found between epilepsy and focal epilepsy (PP.H4.abf = 97.7e-2). MAGMA analysis indicated that SNPs associated with epilepsy and focal epilepsy were functionally localized to zinc-finger-protein-related genes (p < 1.0e-5).3. The genes associated with focal epilepsy were found to have a molecular function of zinc ion binding (FDR = 1.9e-4). Within 4 to 24 hours after experiencing epilepsy, the function of the gene whose expression changed in the rats with focal epilepsy was enriched in the biological process of vascular response (FDR = 4.0e-5), compared to the rats without seizure.
Conclusion
The mechanism of the increased risk of epilepsy caused by zinc may be related to the increase of zinc ion-dependent carbonic anhydrase or the increase of the volume of zinc-rich caudate gray matter.
Cytoplasmic Recycling of rcDNA-containing Capsids Enhances HBV Infection
Authors: Sutradhar Rupchand , Dalal D C 
Published: February 2024

Abstract

Hepatitis B virus (HBV) infection is a deadly liver disease. The main aim of this work is to explore the role of cytoplasmic recycling of rcDNA-containing capsids in the hepatitis B virus (HBV) infection. To this purpose, considering the recycling of capsids, a noval mathematical model is proposed in order to understand the dynamics of this viral infection in a better way. Through a rigorous comparison with experimental data obtained from four chimpanzees, the proposed model exhibits a robust alignment with the dynamics of infection. The effects of three parameters (recycling rate, virus production rate, and volume fraction of newly produced capsids) are examined, revealing an interesting observation: the inclusion of recycling reverses the influence of both virus production rate and the volume fraction of newly produced capsids in infection. A comprehensive global sensitivity analysis is conducted to identify the most positively as well as negatively sensitive parameters for each compartment in the model. In conclusion, this study underscores that the accumulation of rcDNA-containing capsids within the infected hepatocyte is a key factor contributing to the exacerbation of the disease. In addition, another major finding of our study is that due to recycling of capsids, the number of released viruses increases in spite of low virus production rate. In other words, the recycling of capsids acts as a positive feedback loop in the viral infection.
Mathematics Subject Classification (2020) 37N25, 34D23, 34A34
Femtosecond Thin-flap laser assisted in situ keratomileusis for Correction of Post- Penetrating Keratoplasty Ametropia: Long-Term Outcome
Authors: Einollahi Bahram , Rezaei Javad , Sadoughi Mohammad-Mehdi , Feizi Sepehr , Einollahi Neda , Veisi Amir Reza , Hassanpour Kiana 
Published: February 2024

Abstract
Purpose: To evaluate the long-term clinical outcomes of femtosecond thin-flap LASIK (femto-LASIK) for correction of refractive error after penetrating keratoplasty in keratoconus-affected eyes.
Setting: a private ophthalmology clinic
Design: Prospective interventional case series
Methods: This prospective interventional case series enrolled 22 eyes of 22 patients who underwent femto-LASIK for the management of post-penetrating keratoplasty ametropia. The refractive error, uncorrected (UDVA), and corrected (CDVA) distance visual acuities and vector analysis were reported in short-term and long-term period after surgery.
Results: The mean age was 32.7 ± 7.5 years (range, 23 to 47 years) at the surgery time. The average time between PK and femto-LASIK was 42.5 ± 31.7 months. The average follow-up duration after femto-LASIK was 81.2 ± 18.6 months. The mean preoperative UDVA significantly improved from 0.47 ± 0.15 to 0.35 ± 0.14 at 12 months (P=0.048) and 0.4 ± 0.17 at final follow-up exam (P= 0.007). CDVA was 0.22 ± 0.1 at baseline which improved to 0.18 ± 0.15 and 0.15 ± 0.1 logMAR at 12 and 81 months, respectively. (Ps= 0.027, 0.014). The mean cylinder before surgery was -5.04 ± 1.4D which significantly decreased to -1.5 ± 0.8 D at 12 months postoperatively. (P< 0.001). There was a significant increase in refractive astigmatism from 12 months to 81 months postoperatively (-3.1±2.0, P=0.002).
Conclusions: Our findings suggest that femtosecond thin-flap LASIK was an effective and safe procedure to correct refractive error after penetrating keratoplasty. However, some regression was observed at the final exam compared to results achieved immediately postoperatively.
Psychological Factors and Clinical Outcomes after Total Knee Arthroplasty surgery in Patients
Authors: Lathabhavan Remya , Marznaki Zohreh Hosseini , Nukpezah Ruth Nimota , Mudgal Shiv Kumar , Afshar Amir Mahmoud , Khalilizad Majid 
Published: February 2024

Abstract

Background
This study explored insomnia's influence on mental health, general health, and quality of life among total knee arthroplasty (TKA) patients in Northern Iran. It hypothesized that insomnia moderates the link between general and mental health while mediating the connection between general health and quality of life.
Methods
A cross-sectional survey involved 270 patients who completed depression, mental health, quality of life, and insomnia assessments at one week, one month, and three months post-surgery. Structural equation modelling (SEM) was used to analyse the data.
Results
The study included 270 patients. General health positively correlated with mental health (0.21, p < 0.01;0.25, p < 0.01; 0.27, p < 0.001). Whereas general health has shown significant relationship with quality of life in time wave 2 and time wave 3(0.13, p < 0.001;0.17, p < 0.01). Mental health also positively influences the quality of life (0.17, p < 0.01; 0.19, p < 0.001;0.22, p < 0.001). Insomnia negatively moderated the relationship between general health and mental health. Furthermore, insomnia mediated the relationship between general health and quality of life in the later time points. The study highlighted the intricate relationships between general health, mental health, quality of life, and insomnia in TKA patients. General health contributed to improved mental health and quality of life over time. Insomnia acted as both a moderator and mediator, affecting these relationships.
Conclusion
This study sheds light on the complex dynamics affecting TKA outcomes, offering insights for healthcare providers to deliver more comprehensive care for improved patient well-being.
RETRACTED: Safety and clinical efficacy of immune checkpoint inhibitors in advanced gastric cancer in the real world
Authors: Hao Wen , Liu Wenjing , Chang Ruimin , Yang Mi , Xin Kai , Liu Jingxin , Wang Yibing , Ren Meijin , Xie Jiaqi , Yang Yang 
Published: February 2024

Abstract
The authors have requested that this preprint be removed from Research Square.
Psychometric evidence of the Female Sexual Function Index (FSFI) in a Chilean representative sample
Authors: Quintana Gonzalo R. , Ponce Fernando P. 
Published: February 2024

Abstract

Inconsistencies in the Female Sexual Function Index (FSFI) factor structure and current research and clinical guidelines highlight the need for continued exploration of its psychometric evidence. Furthermore, only one study has assessed its measurement invariance (MI), while the only FSFI validation conducted in Chile lacks quality and representativity. Therefore, this study aimed to evaluated the FSFI in a Chilean sample (n = 2595), examining MI across relationship status, age groups, regions of the country, BDSM practices, and paraphilic interests. Confirmatory factor analysis supported a six-factor structure. Criterion-related validity indicated modest-to-moderate effects in satisfaction differences based on relationship status, regions, and BDSM practices. MI was achieved across socio-demographic variables at the factor covariance level. While acknowledging the absence of a clinical group for female sexual dysfunction (FSD) diagnosis, potential pandemic-related impact on scores, online methodology biases, and limited diversity in gender and sexual orientation, our findings supports the FSFI as a valuable research tool for female sexual function and wellbeing. This study significantly expands the applicability of the FSFI and underscores its measurement invariance across sociodemographic factors, as well as its applicability in the Chilean context.
Evaluation of radiotherapy efficacy and prognostic analysis for solid and cystic brain metastases
Authors: sun lu , Lin Shouyu , Bi Jianping , Yuan Zilong , Li Ying , Wei Wei , peng yi , Hu Desheng , Han Guang 
Published: February 2024

Abstract
Purpose
Brain metastases (BMs) are commonly categorized into cystic and solid. Despite advances in treatment modalities, the prognosis for patients with cystic and solid BMs following radiotherapy remains poorly understood. We use a retrospective design to elucidate the disparities in survival between these two patient groups undergoing radiotherapy and identify factors influencing overall survival (OS) of BMs in solid tumor.
Methods
This retrospective study encompasses 212 patients diagnosed with BMs. We meticulously analyzed the clinical characteristics, radiation therapy modalities, and risk factors influencing overall survival among these patients, categorized by BMs type, post-brain radiation therapy.
Results
A statistically significant difference in mOS was observed between the two cohorts (Solid vs. Cystic: 23.1 vs. 14.6 months). Subgroup analysis unveiled distinctions in mOS, particularly in patients with EGFR-mutant lung adenocarcinoma (Solid vs. Cystic: 23.1 vs. 6.43 months). The volume of BMs and the biological effective dose (BED) emerged as significantly prognostic factors for patients with cystic BMs. For patients with solid BMs, fraction dose, BED and the number of BMs were identified as independent prognostic factors for survival.
Conclusion
Brain radiotherapy shows superior survival benefits for lung cancer patients with solid BMs compared to those with cystic BMs, particularly in EGFR-mutant lung cancer. In particular, patients receiving BED ≥ 60Gy have a more favorable prognosis than those receiving BED < 60Gy, regardless of the type of BM (solid or cystic) in lung cancer.
Investigations of the structural, mechanical and optoelectronic attributes of Rb2BB'Tl I6 ( B'= As, Ga) double perovskites for photovoltaics
Authors: Jamil Muhammad , Ain Quratul , Munir Junaid , Murtaza Hudabia , Ghaithan Hamid M. , Ahmed Abdullah Ahmed Ali , Qaid Saif M. H. 
Published: February 2024

Abstract
Double perovskites based on rubidium have demonstrated potential for obtaining high solar cell power conversion efficiencies. Their distinct crystal structure and electrical characteristics influence these materials' potential as effective light absorbers. In this present manuscript, a detailed scrutiny of the physical aspects of halide perovskites Rb2TlAsI6 and Rb2TlGaI6 is presented using density functional theory framework implanted in Wien2K code using. Modified Becke Johnson potential is employed to treat the exchange-correlation effects. A computed tolerance factor, octahedral tilting, and formation energy ensure the structural and thermodynamic stability of given structures. Three independent elastic constants and mechanical properties were computed using the Thomas Charpin method. Ductile nature of Rb2TlAsI6 and brittle nature for Rb2TlGaI6 is revealed from computed mechanical attributes. Electronic properties revealed a direct bandgap (1.09 eV) for Rb2TlAsI6 and an indirect bandgap (1.2 eV) for Rb2TlGaI6. Optical properties indicate high polarization and absorption of incident light, which is suitable for photovoltaic applications in the visible spectrum.
Characterization, evolution, and abiotic stress responses of leucine-rich repeat receptor- like protein kinases in Liriodendron chinense
Authors: Mu Zhiying , Xu Mingyue , Manda Teja , Chen Jinhui , Yang Liming , Hwarari Delight 
Published: February 2024

Abstract

Background
The Liriodendron chinense similar to all other flowering plants is vulnerable to plant extinction due to the severity of the abiotic stresses in the wake of the global climate change. Thereby, affecting its growth and development, and geographical distribution. Nonetheless, the Liriodendron chinense plays an essential role in the socio-economic and ecological sectors. The LRR-RLK genes comprise one of the largest groups of receptor-like kinases in plants, crucial for plant development and stress regulation; and the LRR-RLK genes have not been elucidated in the Liriodendron chinense.
Result
In this study, we identified 232 LchiLRR-RLK genes that were unevenly distributed on 17 chromosomes and 24 contigs, of which 67 paralogous gene pairs portrayed gene linkages; that contributed to LchiLRR-RLK gene family expansion through tandem (35.82%) and segmental (64.18%) duplications. Additionally, the synonymous and nonsynonymous ratios showed that the LchiLRR-RLK genes underwent a purifying or stabilizing selection during the evolutionary process. Investigation in the protein structures and domain conservation exhibited that LchiLRR-RLK carried conserved PK and LRR domains that also promoted their clustering in different subfamilies implicating gene evolutionary conservation. A deeper analysis of LchiLRR-RLK full protein sequences phylogeny showed 13 families that had a common ancestor protein. Interspecies gene collinearity showed more orthologous gene pairs between L. chinense and P. trichocarpa, suggesting various similar biological functions between the two plant species. Analysis of the functional roles of the LchiLRR-RLK genes using the qPCR demonstrated that they are involved in abiotic stress regulation, especially, members of subfamilies VIII, III, and Xa.
Conclusion
Conclusively, the LRR-RLK genes are conserved in the L. chinense and function to regulate the temperature and salt stresses, and this research provides new insights into understanding LchiLRR-RLK genes and their regulatory effects in abiotic stresses.
Application of The Laparoscopic Auxiliary Needle Device in the Treatment of Pediatric Inguinal Hernia: An Eight- year Experience
Authors: Shao Jinwei , Ma Mingdi , Li Zhou , Shi Manyu , Li Zhaozhu , Cui Qingbo 
Published: February 2024

Abstract
Background
Pediatric inguinal hernia is a common surgical condition among children. In this study, we present the application of a self-designed laparoscopic auxiliary needle device (China Patent Number: ZL 201320479515.5) and evaluate its effectiveness in laparoscopic percutaneous extraperitoneal closure in pediatric patients.
Methods
The laparoscopic auxiliary needle device, with a diameter of 1 mm, consists of a cylindrical needle body and a specifically designed front end. Between January 2013 and January 2021, a total of 2075 children with inguinal hernia (1864 males and 211 females, mean age 3.71 ± 2.95 years old) were included in this study. All patients underwent laparoscopic inguinal hernia repair using the laparoscopic auxiliary needle device. Various data were collected including occult inguinal hernia incidence, complications such as recurrence, and follow-up findings.
Results
The entire patient cohort underwent laparoscopic inguinal hernia repair using the laparoscopic auxiliary needle. Pneumoperitoneum lasted 5.1 ± 1.2 minutes in unilateral hernia cases and 11.0 ± 1.3 minutes in bilateral hernia cases. Occult inguinal hernia were found in 981 (47.3%) patients, out of which initially difficult diagnoses were encountered in 516 cases (24.9%). Postoperative follow-up ranged from 3 to 96 months, with two instances (0.1%) of inguinal hernia recurrence reported. Importantly, no patients reported any visible scars or sensations of subcutaneous foreign bodies.
Conclusion
Based on our extensive patient cohort, we assert that our self-designed laparoscopic auxiliary needle device represents a valuable tool for performing laparoscopic inguinal hernia repairs while minimizing abdominal scarring. Additionally, this device offers the additional advantage of detecting contralateral occult inguinal hernia.
Handwashing Sink as a Reservoir of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit: A Prospective Multicenter Study
Authors: Wei Li , Feng Yu , Lin Ji , Kang Xia , Zhuang Hongdi , Wen Hongxia , Ran Shasha , Zheng Lan , Zhang Yujing , Xiang Qian , Liu Yan , Wu Xueqin , Duan Xiaofei , Zhang Wensheng , Li Qu , Guo Hua , Tao Chuanmin , Qiao Fu 
Published: February 2024

Abstract

BackgroundThe extent to which sinks are contaminated by carbapenem-resistantAcinetobacter baumannii(CRAB) in intensive care units (ICUs) and the association between these contaminated sinks and hospital acquired CRAB infections during non-cluster period remains largely unknown .MethodsA prospective multicenter study was performed in 16 ICUs, including 9 general ICUs (GICUs) and 7 neonatal ICUs (NICUs), at 11 tertiary hospitals in Chengdu from March 2019 to January 2020. Sampling of the sinks was performed once, and CRAB clinical isolates recovered within two weeks before and three months after the sampling date were collected. Whole-genome sequencing was used to analyze the isolates and determine their clones.ResultsA total of 789 swabs were collected from 158 sinks, and 16 CRAB isolates were recovered from 16 sinks, resulting in a contamination rate of 10.16% (95% CI, 5.40%~14.83%). Twenty-seven clinical isolates were collected during the study period. The majority (97.67%, 42/43) of the CRAB isolates belonged to ST2 and 36 (83.72%) of them had bothblaOXA−23andblaOXA−66. The 43 strains belonged to 12 clones. One certain clone caused multiple contaminations of 7 sinks in one GICU. Two clones of ST2blaOXA−23andblaOXA−66-carrying sink strains were likely the sources of the two clusters in the two GICUs, respectively. Five ST2blaOXA−23-carrying isolates were found to be a common clone but were recovered from two hospitals. Conclusion: The contamination rate of CRAB in handwashing sinks is high in some local ICUs, and the contaminated sinks can serve as environmental reservoirs for CRAB clusters.