bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales
Authors: Costa Flávia de F. , Souza Rafael S. C. de , Voidaleski Morgana F. , Gomes Renata R. , Reis Guilherme F. , Lima Bruna J. F. de S. , Candido Giovanna Z. , Geraldo Marlon R. , Soares Jade M. B. , Schneider Gabriela X. , Trindade Edvaldo da S. , Bini Israel H. , Moreno Leandro F. , Bombassaro Amanda , Queiroz-Telles Flávio , Raittz Roberto T. , Quan Yu , Arruda Paulo , Attili-Angelis Derlene , de Hoog Sybren , Vicente Vania A. 
Published: October 2023

AbstractSugarcane (Saccharum officinarum, Poaceae) is cultivated on a large scale in (sub)tropical regions such as Brazil and has considerable economic value for sugar and biofuel production. The plant is a rich substrate for endo- and epiphytic fungi. Black yeasts in the family Herpotrichiellaceae (Chaetothyriales) are colonizers of human-dominated habitats, particularly those rich in toxins and hydrocarbon pollutants, and may cause severe infections in susceptible human hosts. The present study assessed the diversity of Herpotrichiellaceae associated with sugarcane, using in silico identification and selective isolation. Using metagenomics, we identified 5833 fungal sequences, while 639 black yeast-like isolates were recovered in vitro. In both strategies, the latter fungi were identified as members of the genera Cladophialophora, Exophiala, and Rhinocladiella (Herpotrichiellaceae), Cyphellophora (Cyphellophoraceae), and Knufia (Trichomeriaceae). In addition, we discovered new species of Cladophialophora and Exophiala from sugarcane and its rhizosphere. The first environmental isolation of Cladophialophora bantiana is particularly noteworthy, because this species up to now is exclusively known from the human host where it mostly causes fatal brain disease in otherwise healthy patients.
The novel approach for non-invasive diagnostic biomarkers from an early stage of NAFLD to advanced fibrosis
Authors: Dudeja Pooja , Pal Taishee , Sharma Aman 
Published: October 2023

Abstract
Background
Non-alcoholic fatty liver disease (NAFLD) is a spectrum of disorders that will be started from more than or equal to 5% of fats deposited into the liver hepatocyte cells and progressively leads to steatosis, further increment in fat deposition, and signature of inflammatory markers which cause the non-alcoholic steatohepatitis (NASH) condition. Due to a lack of diagnosis and effective treatment, NASH is converted into liver cirrhosis or hepatocarcinoma, which indicates the irreversible stage of the disease and finally recommends liver transplantation for patient survival. However, nowadays, several clinical biomarkers are identified, and most of the new biomarkers are in the developmental stage, but still the diagnosis of each stage of fatty liver is unaccomplished. So, in this review article, we try to present all current mechanistic perspectives to find the non-invasive biomarkers which could be the best approach in the future to diagnose fatty liver disease in each stage.

Main text
NAFLD is a growing phase disease if properly not taken care of by the patient. There are certain factors that can make fast progress in the disease stage like NAFLD to advance liver fibrosis or hepatocarcinoma. We describe to the best extent how different types of disease stages in the case of the fatty liver could be diagnosed using non-invasive biomarkers. A certain type of mechanistic pathophysiology approach is used to differentiate each stage of fatty liver disease like serum biomarkers (inflammatory cytokines), lipoproteins, micro-RNAs, gut microbiome-associated biomarkers, lipid droplet-associated perilipins, apolipoprotein E, the role of dihydroceramide, and gene expression studies.

Conclusions
Recent advancements in diagnostic biomarkers research focused on non-invasive methods, but the diagnosis of different stages of fatty liver disease is still inconclusive. We tried to cover all the potential non-invasive biomarkers in our manuscript. This review helps the researchers to develop possible diagnostic biomarkers for each stage of liver disease.
The implications of the COVID-19 pandemic on hepatitis B and C elimination programs in Egypt: current situation and future perspective
Authors: Elsharkawy Aisha , Samir Reham , Abdallah Mohamed , Hassany Mohamed , El-Kassas Mohamed 
Published: October 2023

AbstractHepatitis B virus (HBV) and hepatitis C virus (HCV) are amongst the most common causative agents of viral hepatitis with its severe complications, including liver cirrhosis, decompensation, and hepatocellular carcinoma (HCC). Elimination of viral hepatitis, a significant challenge, has become an adopted global goal with certainly designed targets set by the World Health Assembly to be met by 2030. While many countries, including Egypt, have started executive plans for viral hepatitis elimination and achieved remarkable progress, the emergence of the COVID-19 pandemic has markedly affected all the machinery of the healthcare systems and specifically laid countries off their track in their viral hepatitis elimination process. The pandemic disrupted most healthcare services, and health staff and hospital resources were recruited mainly for managing the crisis, which significantly negatively impacted the management of other less severe diseases, including viral hepatitis. Social distancing and restrictive measures applied by most countries to contain the pandemic have affected medical services offered to patients with hepatitis. All supply chains of medications and vaccinations concerned with treating and preventing viral hepatitis have been markedly compromised. Many efforts and strategies are required to combat the severe and deleterious implications of the pandemic on the management of viral hepatitis worldwide in an attempt to get the situation under control and resume the pathway towards viral hepatitis elimination.
Trends and critical points of Aspergillus contamination along Ethiopian chili postharvest value chain
Authors: Tolera Tariku Hunduma , Tesfaye Anteneh , Alemu Melaku 
Published: October 2023

Abstract
Background
Chili is the most commonly grown spice in Ethiopia and is a high-value crop for household consumption and sale both at domestic and export markets. However, an unsafe level of fungal toxins is becoming a problem leading to challenges in exporting. This study assessed trends, possible points of Aspergillus contamination, and contamination risk factors along the Ethiopian chili postharvest value chain (PVC).

Methods
Chili handling practices, value chain actors, and their respective roles were investigated along the PVC through an exploratory type of research, a participant unstructured observation. A total of 214 individual sample units composed of multiple subsamples consisting of aseptically picked matured red pods (PiPP), dried red pods (DPP), crushed chili (CP), unpacked (UpPPo), and packed chili powder (PaPPo) were randomly collected along the PVC from different major chili growing localities of Ethiopia during 2017/2018 main cropping season. Individual sample units were further homogenized into a fine powder and composited. Aspergillus was analyzed using Aspergillus flavus and parasiticus agar medium. To monitor Aspergillus contamination, trend analysis was done using the mean of count data and biological inference was made in association with stages of operations and postharvest handling practices.

Results
Aspergillus was detected in 44% of PiPP, all (100%) of DPP, CP, UpPPo, and PaPPo. Counts were in the range of 5.00 × 103 to 2.10 × 105 CFU g−1 up along the PVC with fold changes of 19.6, 30, 42, and 38-fold in DPP, CP, UpPPo, and PaPPo, respectively. Nigri (99%), Flavi (85%), and Circumdati (56%) were the most detected sections with relative densities of 50, 29, and 14%, respectively. Postharvest handling practices such as harvesting, sun-drying, and transporting were generally found poor and unhygienic.

Conclusions
Counts of Aspergillus showed gradually increasing trends up along the PVC. The poor and unhygienic handling practices probably contributed to the contamination. Harvesting and direct open sun-drying were likely initial and critical points of contamination while wetting and tight stacking likely contributed to aggravated growth and proliferation of aspergilli leading to further consecutive buildup. Intervention at these stages would make a significant difference.
Evaluation of a 12-week app-guided exercise intervention in patients with knee osteoarthritis (re.flex): a study protocol for a randomized controlled trial
Authors: Dieter Valerie , Martus Peter , Janssen Pia , Krauss Inga 
Published: October 2023

Abstract
Background
Current health care demonstrates an insufficient provision and utilization of physical exercises despite their recommendation as a first-line treatment in clinical guidelines for patients with knee osteoarthritis (OA). Mobile health (m-health) technologies offer new opportunities to guide and monitor home-based exercise programs by using mobile devices and inertial sensors in combination with a digital application (app). This study will evaluate patient benefits resulting from the use of the specific digital health application re.flex for patients with knee OA.

Methods
This monocentric, two-arm, randomized controlled parallel-group trial will evaluate the effectiveness of the app- and sensor-guided exercise program re.flex for patients with moderate-to-severe knee OA. We aim to recruit 200 participants via newspapers, newsletters and information events. Participants will be randomly allocated to the intervention group and the control group in a 1:1 ratio. Participants in the control group will not receive any study intervention or instruction for any change to their previous health care utilization. Despite this, they are allowed to make use of usual care provided by their treating physician. The intervention group comprises a 12-week home training program with three sessions per week in addition to usual care. Exercises will be guided and monitored by use of the training app (re.flex) and two accelerometers that are attached proximally and distally to the affected knee joint. Pre- and postmeasurements will take place at baseline (t0) and after 12 weeks (t1). Primary outcomes will be osteoarthritis-specific pain and physical function measured with the Knee Osteoarthritis Outcome Score (KOOS) subscales Pain and Function in daily living (ADL). Second, further self-reported health outcomes, a performance measurement, app logfiles and safety will be assessed. Intervention effects will be calculated by baseline-adjusted analysis of covariance (ANCOVA) using an intention-to-treat approach. Multiple imputation will be applied.

Discussion
Re.flex can bridge part of the gap between recommendations for strengthening exercises in patients with knee OA and the insufficient actual care situation. This randomized controlled trial is designed to provide conclusions on the effectiveness of the health application re.flex for the population under study and will provide further insight into adherence rates and the safety of its use.

Trial registration
The trial was registered on 20/01/2023 at www.drks.de (ID: DRKS00030932).
THU029 Subcutaneous Self-injections Of Lanreotide Is a Good Alternative For Acromegalic Patients With Long COVID Treated With Anticoagulants
Authors: Rosa Isabella Naves , Naves Luciana Ansaneli 
Published: October 2023

Abstract
Disclosure: I.N. Rosa: None. L.A. Naves: None.
Background: Acromegaly control during pandemics was an important challenge. Telemedicine was a resource to provide health care, associated to electronic prescription sent directly to central pharmacy and drugs delivered to patients' home avoided treatment disruption in 2020 and 2021. The concomitant use of anticoagulants in Covid patients is a major concern because of risk of muscle hematomas. We describe here the replacement of Octreotide LAR to Somatuline Autogel in long Covid acromegalic patients with thromboembolic co-morbidities. Methods: We describe a sample of acromegalic patients, diagnosed with long Covid and thromboembolic co-morbidities. All patients were previously submitted to transsphenoidal surgery for somatotropic tumors and were controlled by mensal injections of octreotide LAR. Outpatient management was remodeled to simplify access to care by (1) adoption of virtual meetings; (2) collection of blood samples at home; (3) abolishment of printed prescription and provision of electronic files directly to central pharmacy; and (4) replacement of intramuscular injections for subcutaneous self injection of Lanreotide- Autogel. Results: From our cohort of 128 acromegalic patients, coronavirus disease (COVID-19) was confirmed in18%, although, long COVID, and use of anticoagulant drugs for more than 4 months was observed in 8 cases, 5 women, median age 68 years. Patients adopted self injections monthly and maintained disease control in 75% of cases with good adherence. Local side-effects, as injection-site pain, equimosis were observed less frequently than reported by the patients in previous use of Octreotide LAR. Conclusion: Telemedicine was a feasible tool in acromegalic patients with Long Covid. The replacement of intramuscular injections of Octreotide LAR for Lanreotide Autogel self injected subcutaneously allowed stability of disease control and provided an alternative to injections administered in the health care
Presentation: Thursday, June 15, 2023
FRI348 Ectopic Posterior Pituitary As A Cause Of Panhypopituitarism Presenting With Hyponatremia
Authors: Min Choo Charlotte Hui 
Published: October 2023

Abstract
Disclosure: C. Choo: None.
Background: Ectopic posterior pituitary gland (EPP) can occur from defective embryogenesis affecting neuronal migration, or from neurodegeneration of the hypothalamic nuclei. While isolated growth hormone deficiency is most common, it is also associated with pan-hypopituitarism. The posterior pituitary function is often preserved. Clinical Case: We report a case of pan-hypopituitarism from congenital EPP, presenting with hyponatremia. A 63-year-old male with hyperlipidemia was admitted for gastroenteritis. Serum sodium was 106mmol/L [reference range 136-146mmol/L] on admission but he was otherwise asymptomatic and haemodynamically stable. This initially improved with intravenous fluids, however subsequently stagnated at 121mmol/L despite achieving euvolemia. Thyroid and cortisol axes were evaluated in view of persistent hyponatraemia. 8am cortisol was low at 119nmol/L with inappropriately normal ACTH 13.9 pg/mL [reference range 7.2 – 62.3 pg/mL]. Thyroid function test showed central hypothyroidism consistently on repeat testing with a low fT4 of 6.1 pmol/L and 7.7 pmol/L [reference range 8.8-14.4pmol/L] and inappropriately normal TSH of 2.98MU/L and 2.41MU/L respectively [reference range 0.65-3.70MU/L]. 1mcg short synacthen test done following suspicion of hypocortisolism peaked at 468 nmol/L. He had no symptoms or signs of hypocortisolism or hypothyroidism. He was a bachelor and had clinical features of hypogonadism including short stature (142cm), high-pitched voice, scarce sexual hair and pubescent sized testes. His mid-parental height is unknown as his parents were deceased. FSH, LH, testosterone and IGF-1 were all undetectable. Prolactin was otherwise normal 9.4ug/L [reference range 4.2-16.4ug/L]. Radiographs of the forearm showed unfused epiphyseal plates at the distal radius and ulna. Thus, the diagnosis of pan-hypopituitarism was made. Magnetic resonance imaging of the pituitary revealed a T1 hyperintense nodule at the floor of the third ventricle, suggestive of ectopic posterior pituitary. There was otherwise minimal normally enhancing tissue in the pituitary fossa, and the posterior pituitary bright spot and infundibulum were absent. He was started on hydrocortisone and subsequently levothyroxine replacement, with resolution of hyponatraemia (serum sodium 139nmol/L). He did not develop polyuria or polydipsia to suggest diabetes insipidus. Due to financial concerns however, he declined growth hormone and testosterone replacement therapy. Conclusion: Most reports of EPP are in the pediatric population often presenting with short stature. This was a seemingly ordinary case of hyponatraemia initially deemed to be from hypovolaemia. His atypical clinical features led to the eventual diagnosis of EPP with panhypopituitarism at a rather late age, highlighting the importance of a thorough clinical examination necessitating further evaluation.
Presentation: Friday, June 16, 2023
FRI391 A Metabolomics Analysis Of Moderate Energy Restriction In Healthy Lean Women
Authors: Levine Lindsay , Overdahl Kirsten , Molsberry Samantha , Shekhar Skand , Jarmusch Alan , Hall Janet E 
Published: October 2023

Abstract
Disclosure: L. Levine: None. K. Overdahl: None. S. Molsberry: None. S. Shekhar: None. A. Jarmusch: None. J.E. Hall: None.
Introduction: Metabolomics can elucidate metabolic responses to dietary interventions and has been used in energy restriction studies, primarily in overweight/obese men and women. Although the menstrual cycle affects the metabolome, most dietary intervention studies have not controlled for menstrual cycle phase, nor have they examined the effect of moderate, short-term energy restriction on the metabolic profile of healthy, normal-weight women. Objective: To characterize metabolomic changes in response to a 55% reduction in dietary intake during the early follicular phase of the menstrual cycle. Methods: Study participants included 19 healthy women aged 18-28 years who were of normal BMI and fitness, not restricting nutritional intake and with biochemically confirmed regular ovulatory menstrual cycles. Participants underwent 5-day dietary interventions in the early follicular phase of two menstrual cycles. The first diet consisted of neutral energy availability (NEA; 45 kcal/kg*lean body mass/day) and the second of deficient energy availability (DEA; 20 kcal/kg*lean body mass/day) without changes in macronutrient composition. On the 5th day of each diet, serum was collected at ∼8am following an overnight fast, and immediately before and every 10 minutes for 60 minutes after the consumption of a standardized isocaloric snack at ∼4pm. Untargeted metabolomic profiling was performed using ultra-high performance liquid chromatography/mass spectroscopy. Univariate statistics were performed on all metabolites, using t-tests with Benjamini-Hochberg false discovery rate adjusted p-values to account for multiple testing (p-value ≤ 0.05). Log2-fold change was calculated for all chemical features. Results: At baseline, participants had a weight of 61.9±2.75 kg (mean±SEM), BMI of 23.96±0.69 kg/m2 (mean±SEM), and percent body fat of 28.01±1.61% (mean±SEM). DEA resulted in a 1.25±0.13 kg (mean±SEM) decrease in body weight (p&lt;0.01). While few statistically significant differences were present between fasted NEA and DEA samples, numerous chemical features were significantly upregulated in DEA post-snack samples. Specifically, 11 carnitines, 7 phosphocholines, and 8 fatty acids or fatty acid intermediates exhibited a log2fold change of 0.5 or higher (&gt;40% increase; p ≤ 0.05) in post-snack DEA samples compared to NEA samples. Conclusion: These results strongly suggest that following isocaloric intake, moderate energy restriction in normal-weight, healthy women is associated not only with the use of fat as an energy source, but also with cellular membrane breakdown.
Presentation: Friday, June 16, 2023
FRI395 Ovary-derived Hydrogels Elucidate The Matrisome Properties That May Influence Folliculogenesis
Authors: McDowell Hannah B , Henning Nathaniel , Laronda Monica M 
Published: October 2023

Abstract
Disclosure: H.B. McDowell: None. N. Henning: None. M.M. Laronda: None.
Chemotherapy treatments can be gonadotoxic and lead to loss of fertility and hormone function. Currently, the only pre-treatment strategy available to preserve fertility for prepubescent individuals is ovarian tissue cryopreservation (OTC) and the only option to restore fertility or hormone function is through ovarian tissue transplantation (OTT). Although in most cases OTT restores endocrine function, the tissue functions for an average of 2-5 years. To extend tissue function, the development of a bioengineered ovary that supports long-term follicle growth and endocrine function is critical. Tissue longevity depends on the number of primordial follicles (PMF) and their activation rate. While most PMFs survive the cryopreservation process, 80% are lost shortly after OTT. In bovine ovaries, the cortex, housing PMFs, is 8.5 times more rigid than deep medulla and is comprised of differential matrisome proteins. As mono-ovulatory animals, their ovaries closely mimic humans, making them a suitable model. Here, we developed decellularized extracellular matrix (dECM) hydrogels from bovine cortical tissue (c-dECM) and medullary tissue (m-dECM). Using atomic force microscopy, we determined that c-dECM and m-dECM did not display significantly different rigidities (537Pa, 468Pa). We found that the concentration of COL1, COL4, DCN, and VTN in the dECM hydrogels recapitulated what is seen in native bovine ovarian compartments. Therefore, we hypothesized that differences observed in downstream applications of these gels are due to differences in biochemical cues - or protein composition and relative abundance - in the ovarian ECM. The chemical and structural properties of ECM proteins play an integral role in cellular behavior. Therefore, we performed second harmonic generation scanning microscopy and determined that c-dECM hydrogels have significantly different fiber architecture when compared to m-dECM hydrogels. m-dECM hydrogels had significantly wider and longer fibers than c-dECM (6.22,6.419 and 38.22, 38.84 pixel/ppm microns) and displayed a lower fiber angle alignment (91.01°,87.95°). We assessed whether compartmental c-dECM and m-dECM hydrogels could drive differential expression patterns of downstream candidate genes. We then depleted our gels of proteins of interest using magnetic-activated protein filtration (MAPF). We found that our depleted gels modulate expression of genes associated with regulating follicle growth. Our work employed a novel method for examining the contribution of matrisome proteins in folliculogenesis. We anticipate our work to be influential in studying matrisome-dependent biochemical cues within other organ systems. IMPACT: Determining how the matrisome of the ovary can influence follicle activation will contribute critical data on how we can extend tissue function in OTT.
Presentation: Friday, June 16, 2023