bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_00011640 inhibits lung adenocarcinoma by targeting miR-942
Authors: Han Huize , Xu Kandi , Du Xueqing , Le Van Minh , Lin Hongxia , Don Ocholi , Ni Lei , Shi Guochao , Zhou Ling 
Published: January 2024

Abstract
Objectives: To understand the role of circular RNA (circRNA) hsa_circ_0001640 in lung adenocarcinoma.
Methods: We used high-throughput sequencing and qRT-PCR to examine the functional roles of hsa_circ_0001640 in lung adenocarcinoma (LUAD). Expriment in vivo and in vitro was performed. The role of hsa_circ_0001640 in LUAD cells were evaluated by CCK-8 test, cell cycle analysis, clonal formation, transwell assay and wound healing assay. The relative protein levels were measured by western blot and immunofluorescence. Clinical significance of hsa_circ_0001640 was assessed by paired t test, independent sample t test, one-way ANOVA, and COX logistic regression.
Results: We observed that hsa_circ_0001640 was downregulated in LUAD and was negatively correlated with clinical stage, lymph node invasion and NSE, and it was a protective factor of OS of LUAD patients after surgery. In experiment in vitro, hsa_circ_0001640 could inhibit proliferation and invasion of H1975 and H1299 cells, and more cells remained in G1 phase with upregulation of hsa_circ_0001640. Hsa_circ_0001640 was proved to be combined with miR-942 as ceRNA, and thus suppressing the promotive effect of miR-942 in LUAD. Hsa_circ_0001640 upregulated e-cadherin and down-regulated fibronectin, indicating an inhibiting effect of hsa_circ_0001640 on EMT. Hsa_circ_0001640 was positively associated with GSK3β and NKIRAS2, suggesting that hsa_circ_0001640 was a potential suppressor in Wnt and NF-κB pathway.
Conclusion: Hsa_circ_0001640 was a tumor suppressor in LUAD by targeting miR-942 as a ceRNA, leading to upregulation of GSK3β and NKIRAS2.
Identification of a prognostic gene signature based on invasion-related genes in breast cancer
Authors: Niu Yicong , Zhu Xinliang , Ma Dachang , Li Xun , Ji Kun 
Published: January 2024

Abstract
Purpose:
This study focused on establishing an invasion-related prognosis prediction model for breast cancer (BC).
Methods:
mRNA expression profiles and corresponding clinical information were collected from BC patients in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Thereafter, we identified invasion-related genes based on from Cancer SEA database. Invasion-related differentially expressed genes (DEGs) were identified through differential expression analysis. In addition, a risk model was built on the basis of univariate Cox regression and least absolute shrinkage and selection operator (LASSO) analysis for the TCGA cohort. Moreover, GEO-derived BC patients were used in verification. Besides, relation of risk model with tumor microenvironment was analyzed using the ESTIMATE and CIBERSORT algorithms. This was verified using Quantitative Real-time PCR (RT-qPCR).
Results:
Altogether75 invasion-related DEGs were identified in BC versus control samples. KRT19, PSME2, HMGB3, MRPL13, and SHCBP1 were identified as prognostic signatures for the risk model. In line with the signature-based risk scores, we classified all patients as low- or high-risk group. In training and validation sets, Kaplan-Meier survival and receiver operating characteristic (ROC)analyses verified that our as-constructed 5-gene signature performed well in prediction. MRPL13 and KRT-19 showed significantly increased expression, whereas SHCBP1 showed decreased expression in BC samples compared to that in normal samples. The ESTIMATE and CIBERSORT algorithms revealed different immune statuses of both riskgroups.
Conclusion:
According to our findings, the prognosis prediction model constructed by incorporating 5 invasion-related genes is feasible in predicting BC prognosis.
Visual outcomes following bilateral implantation of a Non-diffractive Wavefront-shaping Extended Depth of Focus Toric Intraocular Lens
Authors: LEE Mun Wai 
Published: January 2024

Abstract

Purpose: To evaluate the clinical outcomes following bilateral implantation of the AcrySof™ IQ Vivity™ toric extended depth of focus (EDOF) intraocular lens (IOL).
Design: Prospective interventional case series
Methods: Patients with bilateral significant cataracts and pre-existing corneal astigmatism underwent cataract surgery and implantation with the AcrySof™ IQ Vivity™ Toric IOL. Dominant eyes were targeted at emmetropia and non-dominant eyes at -0.50D. Primary endpoints were binocular uncorrected distance (UDVA), intermediate (UIVA at 66cm) and near (UNVA at 40cm) acuities at 3 months. Secondary outcomes were corrected distance (CDVA), distance corrected intermediate (DCIVA) and distance corrected near (DCNVA), refractive predictability, rotational stability, binocular defocus curve, contrast sensitivity, Questionnaire for Visual Disturbances (QUVID) and VF-14 questionnaire scores. All visual acuities were converted to logarithm of minimum angle of resolution (logMAR) for analysis.
Results: 30 patients underwent uneventful phacoemulsification. The mean binocular UDVA, UIVA and UNVA were 0.06 + 0.12, 0.11 + 0.10 and 0.26 + 0.10 respectively. The mean refractive spherical equivalent (MRSE) for dominant and non-dominant eyes were -0.07D + 0.27 and -0.12D +0.54 respectively. 82.4% of dominant eyes and 84.6% of non-dominant eyes within 0.50D of target respectively. The mean IOL rotation was 3.85° + 5.09 with 86.7% of eyes with less than 5° of rotation. 26.7%, 20% and 36.7% of patients reported starbursts, haloes and glare respectively. The mean VF-14 score was 91.77.
Conclusion: Bilateral implantation of the AcrySof™ IQ Vivity™ Toric IOL resulted in very good unaided visual acuities for far and intermediate distance with functional near vision. Dysphotopsias were reported but despite this, a high level of visual function was achieved.
Correlation between angiopoietin-2 and layered plaques in patients with acute coronary syndrome
Authors: Wen Jiaojiao , Yang Changlong , Deng Chancui , Yang Xueyuan , Xu Xinyu , Bai Zhixun , Wang Xi , Hu Xingwei , Shi Bei , Ma Yi 
Published: January 2024

Abstract
Objective
Through a retrospective analysis of patients diagnosed with Acute coronary syndrome (ACS), relationships between Angiopoietin-2 (Ang2) and Optical coherence tomography (OCT) were investigated, especially in layered plaque.
Methods
In the analysis of patients’OCT image with ACS before operation, a total of 101 patients and 107 target vessels participated. Moreover, patients were divided into layered plaque (LP) group and non-layered plaque (NLP) group in accordance with whether target lesions of the patients had a layered plaque. Furthermore, Ang2 concentration was determined by ELISA. Binary Logistic regression model was employed to determine relationship between Ang2 and layered plaques, and ability of Ang2 to predict layered plaques as target lesions of ACS was determined by Receiver Operating Characteristic(ROC)curve.
Results
Between LP and NLP groups, there were substantial differences in Ang2 concentration. Besides, results of univariate and multivariate analysis indicated that Ang2 was an independent risk factor for layered plaques. Except for that, ROC illustrated that AUC of Ang2 level for predicting layered plaques, and optimum cut-off value was determined by Jorden index (15.045ng/mL).
Conclusions
In comparison with NLP groups, Ang2 levels in patients with ACS in the LP group were substantially higher than those in the NLP group. Other than that, concentration of Ang2 emerged as a standalone predictor for layered plaques.
The property of modified bovine bone glue as an environmental additive in water-based drilling fluids
Authors: Du Weichao , Song Bingqian , Huang Xianbin , Ni Jiajia , Fan Jing 
Published: January 2024

Abstract

At present, animal bone glue has widely used in many fields, but there are no researches been reported in drilling fluids. In this paper, an environmental water-based drilling fluids additive bromoethane modified bone glue (BG) was developed by using bovine bone glue and bromoethane as raw materials, anhydrous ethanol as solvent, sodium hydroxide as alkaline hydrolysis agent and sodium carbonate as a system pH regulator. The inhibition and filtration performance of BG were evaluated. When 4% BG was added, the linear swelling rate was decreased from 50.2%% to 38.2%. The temperature resistance of BG was investigated, and results showed that BG showed good inhibition at 130°C. Microstructure of BG was observed by scanning electron microscope (SEM), it can be seen that BG structure was stabled. Inhibition mechanism of BG in the drilling fluids was studied by infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Zeta potential and thermogravimetric (TG) analysis. Results revealed that its main functional groups were adsorbed in the clay. TGA showed that when 4% BG was added, the basic slurry's thermal stability was improved, and Zeta potential analysis revealed that when 4% BG was added, the drilling fluids' particle size has been declined from 1.251 µm to 0.749 µm, which indicated that the BG can inhibit clay hydration swelling dispersion.
Cost-effectiveness analysis of haemodialysis and comprehensive conservative care in managing end-stage renal disease in Abuja, Nigeria
Authors: Agada-Amade Yakubu Adole , Ogbuabor Daniel Chukwuemeka , Obikeze Eric , Eborieme Ejemai , Onwujekwe Obinna Emmanuel 
Published: January 2024

Abstract

Background
Managing end-stage renal disease (ESRD) is cost-intensive and unaffordable for most people in low and middle-income countries such as Nigeria. Nonetheless, the benefit package of health insurance schemes excludes chronic kidney disease in Nigeria. This study aimed to determine the cost-effectiveness of haemodialysis compared to comprehensive conservative care (CCC) in managing ESRD patients in Nigeria.
Methods
Using direct costs, we conducted a cost-utility analysis of haemodialysis versus CCC from the provider and patient perspectives. The effectiveness of the two alternatives was determined using Disability-Adjusted Life Years (DALYs) averted. We discounted DALYs at 3%, but not costs, because costs were cross-sectional data collected within a year. We used average and incremental cost-effectiveness ratios to compare the cost-effectiveness of the two alternatives. We used one-way sensitivity analysis to assess the robustness of the results.
Results
The unit costs of providing haemodialysis and CCC were $152.21 and $72.44, respectively. The discounted mean DALYs averted per person were $151.7 and $111.1 per DALY averted for haemodialysis and CCC patients, respectively. The average cost-effectiveness ratios were $1.00 and $0.65 per DALY averted for haemodialysis and CCC patients, respectively. Compared to CCC, the incremental cost-effectiveness ratio (ICER) of haemodialysis is $1.97 per DALY averted.
Conclusion
Haemodialysis is substantially more effective and highly cost-effective than CCC for managing ESRD in Nigeria. The findings endorse increasing the coverage of haemodialysis in Nigeria's universal health coverage schemes to make haemodialysis more accessible for ESRD patients needing sustainable kidney care.
Keratin-based Nanoparticles for Oral Delivery of Insulin
Authors: Wang Yunyun , Song Wangdi , Xue Shengnan , Gao Bo , Zhang Yan , Zhang Genlin 
Published: January 2024

Abstract
Successful oral insulin administration can considerably enhance the quality of life (QOL) of diabetes patients who must frequently take insulin injections. Oral insulin administration, on the other hand, is seriously hampered by gastrointestinal enzymes, wide pH range, mucus, and mucosal layers, which limits insulin oral bioavailability to ≤ 2%. Herein, we prepared a simple, inexpensive and safe keratin-based nanoparticles (NPs) to encapsulate sufficient insulin to realize highly efficient oral insulin delivery. The optimized NPs (β-CD-K-IN-DG) achieved high insulin loading (encapsulation efficiency (98.52%) and loading capacity (32.81%)) while protecting insulin from acid and enzymatic degradation. In vivo results confirm that β-CD-K-IN-DG prolonged the residence time of insulin and controlled insulin release under physiological conditions, leading to a notable hypoglycemic effect and a high oral bioavailability of 12.27%. Our work demonstrates that keratin-based NPs can protect insulin from the gastric environment and overcome the intestinal barrier, thus providing the possibility for oral insulin delivery.
Development and validation nomograms to predict long-term survival in patients with locally advanced rectal adenocarcinoma after surgery: a population-based study
Authors: Ma Cheng , Wu Chengjun , Liu Yangsui , Song Tao , Zhou Yun , Zhang Yifan 
Published: January 2024

Abstract
Background: This study aimed to develop predictive nomograms for long-term cancer-specific survival (CSS) and overall survival (OS) in patients diagnosed with locally advanced rectal adenocarcinoma (LARA).
Methods: Patients diagnosed with LARA between 2004 and 2015 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. The patients were randomly divided into training and validation cohorts. Optimal cutoff values for age, lymph node ratio (LNR), and tumor size were determined using X-tile. Univariate and multivariate Cox regression analyses were also conducted to identify independent factors associated with CSS and OS, and these factors were used to construct the nomograms. The performance of nomogram was assessed using the concordance index (C-index), calibration curve, receiver operating characteristic (ROC) curve, and decision curve analysis (DCA). External validation was performed at a single center in China. Furthermore, the predictive performance of the nomograms was compared with that of the 8th edition of the American Joint Committee on Cancer (AJCC) tumor-node-metastasis (TNM) staging system.
Results: This study included a total of 4900 patients diagnosed with LARA from the SEER database, with an additional 116 patients composing the external validation cohort from a single institution in China. The determined optimal cutoff values for age, LNR, and tumor size were 67, 17.1%, and 62, respectively. Cox regression analyses revealed age, race, T and N stage, carcinoembryonic antigen (CEA) levels, tumor size and differentiation, chemoradiotherapy, perineural invasion, and the LNR as independent prognostic factors for both CSS and OS. The C-indexes of the long-term survival nomograms in the training, internal validation, and external validation sets were 0.713 (0.676-0.750), 0.707 (0.670-0.744), and 0.702 (0.600-0.804) for CSS, and 0.700 (0.669-0.731), 0.700 (0.651-0.749), and 0.705 (0.631-0.779) for OS, respectively. The predictive performance of the nomograms was superior to that of the 8th edition of the AJCC TNM staging system.
Conclusions: We established and validated novel nomograms for more precise prediction of CSS and OS in patients with LARA, and the predictive power could guide prognostic prediction and therapeutic decisions.
MYBL2: a novel aggressive biomarker in IDH-mutant Astrocytoma
Authors: Sugur Harsha S. , Rao Shilpa , Arivazhagan Arimappamagan , Mehta Bhupesh , Gayathri Peri Sai , Santosh Vani 
Published: January 2024

Abstract
Purpose:Astrocytoma, IDH-mutant includes CNS WHO grades 2 (A2), 3(A3) and 4(A4). A3 patients have a heterogenous clinical outcome that cannot be fully explained with existing molecular biomarkers. In our previous study we found MYBL2 mRNA to be upregulated in A3 and associated with poor patient outcome. In this study, we aimed to understand the role of MYBL2 in the pathobiology of A3 and clinical course of these patients.
Methods:Protein expression of MYBL2 was investigated on a cohort of A3 and controls using immunohistochemistry (IHC). Its expression was evaluated in paired primary and recurrent samples of A3 and in A2 and A4 tumors. We also assessed p53 immunoreactivity, an important modulator of MYBL2. Functional role of MYBL2 was studied in vitro in three malignant astrocytoma cell-lines [SW1088 and SW783-Secondary cell lines and A3 Primary cell line] using a siRNA knock down approach.
Results:MYBL2 median labelling index (LI) was 15% (0-50%) in A3. Higher MYBL2-LI was associated with shorter PFS (P=0.044) and OS (P=0.017). MYBL2 LI was higher in recurrent tumors compared to primary and in A3 and A4, compared to A2 (P<0.05). p53 LI correlated positively with MYBL2 LI (p=0.026). MYBL2 knockdown was associated with reduced tumor cell proliferation, invasion, and migration.
Conclusion:Our study identified MYBL2 as a novel aggressive biomarker associated with poor prognosis in A3 and with tumor progression. MYBL2 facilitates tumor cell proliferation, migration, and invasion of malignant astrocytoma cells in vitro. Taken together, MYBL2 can be considered as a potentially useful prognostic marker for A3 tumors.
Case report: Mechanical thrombectomy using stent retriever devices in deep cerebral venous thrombosis. When anticoagulation fails
Authors: Álvaro Martínez Martín , Francisco Hernández Fernández , David Molina Nuevo Juan , Blanca Serrano Serrano , Tomás Segura 
Published: January 2024

Abstract

Background:
Treatment of cerebral venous thrombosis has traditionally been based on anticoagulant therapy. However, in certain circumstances, such as deep cerebral venous thrombosis, anticoagulation may be insufficient, so endovascular treatment by mechanical thrombectomy has been used for some years. There is currently no clear indication of which device is the gold standard in the endovascular treatment of cerebral venous thrombosis, although stent retriever devices are the most commonly used.
Case presentation:
We describe two cases of deep cerebral venous thrombosis refractory to anticoagulant treatment treated by mechanical thrombectomy with stent retriever devices, one of which has not been described to date as being used in the treatment of cerebral venous thrombosis (Tiger XL®).
Conclusions:
Some situations in like deep cerebral venous thrombosis may require endovascular treatment with mechanical thrombectomy if anticoagulation fails, with increasing evidence that it improves vital and functional prognosis.
Whole-genome Sequence of Bacillus Velezensis D-18, a Probiotic Bacteria for Aquaculture
Authors: Monzón-Atienza Luis , Lorenzo-Felipe Alvaro , Bravo Jimena , Gómez-Mercader Antonio , Torrecillas Silvia , Galindo-Villegas Jorge , Montero Daniel , Acosta Felix 
Published: January 2024

Abstract
Bacillus velezensis D-18 isolated from a wastewater sample collected from a fish farm, In a previous experiment, Bacillus velezensis D-18 had demonstrated its ability to be a good candidate as probiotic in aquaculture, However, information on its genomic content is lacking. This is the complete genome assembly of Bacillus velezensis D18 using Illumina paired-ends sequencing, which resulted in a 21 contigs assembly of 3.9 Mb. About 4,179 protein-coding genes, 84 encode RNAs were predicted from this assembly.
Implementation of a community-based breastfeeding support intervention to prolong duration of and reduce social inequality in exclusive breastfeeding: a mixed-methods systematic process evaluation in a cluster-randomised trial
Authors: Rossau Henriette Knold , Gadeberg Anne Kristine , Strandberg-Larsen Katrine , Nilsson Ingrid Maria Susanne , Villadsen Sarah Fredsted 
Published: January 2024

Abstract

Background
Breastfeeding is a powerful public health intervention that produce long-term health benefits. Still, in high-income countries such as Denmark, breastfeeding rates are suboptimal and distributed unequally across socio-economic positions. The ‘Breastfeeding – a good start together’ intervention to promote longer duration of and reduce social inequality in exclusive breastfeeding, was rolled out in a cluster-randomised trial during 2022–2023 in a sample of 21 municipalities in two Danish regions. A process evaluation was conducted to assess the fidelity and quality of the implementation and identify possible contextual factors that might have impacted the proposed mechanisms of change.
Methods
The Medical Research Counsel framework for conducting process evaluations guided the study, which was conducted using mixed-methods in a convergence design. Quantitative data: contextual mapping survey (n = 20), health visitor survey (n = 284), health visitor records from 20 clusters and intervention website statistics. Qualitative data: dialogue meetings (n = 7), focus groups (n = 3) and interviews (n = 8).
Results
Overall, the intervention was delivered as planned to intended recipients, with few exceptions. Health visitors reacted positively to the intervention, which they expressed fitted well within their usual practice and which they believed to enhance families’ chances for breastfeeding. Mothers expressed having received the intervention, with few exceptions, and reacted positively to the intervention. Health visitors worried about stigmatisation of the mothers receiving the intensified intervention, however none of the interviewed mothers had felt stigmatised. Contextual factors impacting the intervention implementation and mechanisms were staff- and management turnover, project infrastructure and mothers’ context, including resources, social network and previous experiences.
Conclusions
The overall fidelity of the intervention delivery was high. Health visitors and families responded well to the intervention. Interventions aimed at enabling health care providers to deliver simplified and structured breastfeeding support, in accordance with the support in other sectors of the health care system, may be a means to increase breastfeeding rates and reduce social inequality in breastfeeding, also in international contexts.
Trial registration
Clinical Trials: NCT05311631. First posted April 5, 2022.
Determination of the optimum number of water meter test flow rates in South Africa
Authors: Ncube Mthokozisi , Sibanda Fahlani , Ilemobade Adesola A. 
Published: January 2024

Abstract

Metering errors are one of the main contributors of apparent losses, therefore the determination of the optimum number of flow rates to reproduce the meter error curve is of critical importance. The objective of this study was to determine the optimum number of flow rates suitable for testing water meters in South Africa. This was achieved by accessing data from two local studies that tested 323 used water meters over 10 flow rates. The used water meters had been removed from the field and tested in the laboratory. The data from the laboratory tests were then further analysed by recalculating the weighted error for different flow rate combinations. The optimum number of flow rates, which was found to be 7, was the combination that minimised the weighted error of the tested meters. This study therefore recommends increasing the number of flow rates prescribed by the South African National Standard 1529-1:2019 from three flow rates (qmin, qt and qp) to 7 flow rates, namely 7, 15, 30, 60, 120, 1500 and 3,000 l/h.