bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrospun nylon 6/hyaluronic acid/chitosan bioactive nanofibrous composite as a potential antibacterial wound dressing
Authors: Foroozandeh Amin , Shakiba Mohamadreza , Zamani Amirhosein , Tajiki Alireza , Sheikhi Mehdi , Pourmadadi Mehrab , Pahnavar Zohreh , Rahmani Erfan , Aghababaei Nafiseh , Amoli Hossein Salar , Abdouss Majid 
Published: January 2024

AbstractHyaluronic acid (HA) and chitosan (CS), as natural biomaterials, display excellent biocompatibility and stimulate the growth and proliferation of fibroblasts. Furthermore, nylon 6 (N6) is a low‐cost polymer with good compatibility with human tissues and high mechanical stability. In this study, HA and CS were applied to modify N6 nanofibrous mat (N6/HA/CS) for potential wound dressing. N6/HA/CS nanofibrous composite mats were developed using a simple one‐step electrospinning technique at different CS concentrations of 1, 2, and 3 wt%. The results demonstrated that incorporating HA and CS into N6 resulted in increased hydrophilicity, as well as favorable physical and mechanical properties. In addition, the minimum inhibitory concentration and (MIC) optical density techniques were used to determine the antibacterial properties of N6/HA/CS nanofibrous composite mats, and the results demonstrated that the composites could markedly inhibit the growth of Gram‐positive bacteria Staphylococcus aureus and Gram‐negative bacteria Escherichia coli. Because of its superior mechanical properties, substantial antimicrobial effects, and hydrophilic surface, N6/HA/CS at 2 wt% of CS (N6/HA/CS2) was chosen as the most suitable nanofibrous mat. The swelling, porosity, gel content, and in vitro degradation studies imply that N6/HA/CS2 nanofibrous composite mat has proper moisture retention and biodegradability. Furthermore, the N6/HA/CS2 nanofibrous composite mat was discovered to be nontoxic to L929 fibroblast cells and to even improve cell proliferation. Based on the findings, this research offers a simple and rapid method for creating material that could be utilized as prospective wound dressings in clinical environments.
Safety and efficacy of peripheral blood stem cells collection in healthy children and pediatric patients with thalassemia major weighing 20 kg or less
Authors: Chen Libai , Wen Jianyun , Xu Xiaoxiao , Du Jing , Ruan Yongsheng , Feng Xiaoqin , Li Juan , He Yuelin , Wu Xuedong 
Published: January 2024

AbstractBackgroundPeripheral blood stem cell (PBSC) collection in children poses challenges due to their small size, low body weight (BW), and unique pediatric physiology, especially among children weighing 20 kg (kg) or less.MethodsPBSC collection data of both healthy children and patients with thalassemia major (TM) weighing 20 kg or less between January 2013 and December 2020 were reviewed. Moreover, PBSCs characteristics along with various aspects of efficiency and safety between healthy donors and patients with TM were compared.ResultsA total of 262 PBSC procedures were performed on 255 children. Of these, 91 procedures were carried out on 85 allogeneic healthy donors, and 171 auto‐backup collections were performed on 170 patients with TM to ensure PBSC availability and prevent transplantation failure. A minimum pre‐apheresis hemoglobin (HGB) level of 60 g/L was discovered to be safe and feasible in patients with TM. The median CD34+ cell dose in the PBSC product during the initial apheresis procedure was higher in healthy donors compared to patients with TM (7.29 ± 5.28 × 106 cells/kg vs5.88 ± 4.23 × 106 cells/kg, P = .043). The total CD34+ cells/kg recipient weight exhibited a positive correlation with pre‐apheresis monocyte counts, but a negative correlation with donor weight. Apheresis significantly reduced hematocrit and platelet counts in the allogeneic group compared to the autologous group. Patients with TM experienced a higher occurrence of bone pain related to granulocyte colony‐stimulating factor treatment. Notably, no serious complications related to PBSCs mobilization, central venous catheter placement, or the apheresis procedure were observed in either group.ConclusionsPBSCs collection was both safe and effective in healthy children and pediatric patients with TM weighing 20 kg or less.
Relevance of pyroptosis‐associated genes in nasopharyngeal carcinoma diagnosis and subtype classification
Authors: Wang Yan , Zou Yuxia , Chen Xianghui , Wang Xiaoyan , Zheng Hao , Ye Qing 
Published: January 2024

AbstractBackgroundNasopharyngeal carcinoma (NPC) is a highly aggressive and metastatic malignancy originating in the nasopharyngeal tissue. Pyroptosis is a relatively newly discovered, regulated form of necrotic cell death induced by inflammatory caspases that is associated with a variety of diseases. However, the role and mechanism of pyroptosis in NPC are not fully understood.MethodsWe analyzed the differential expression of pyroptosis‐related genes (PRGs) between patients with and without NPC from the GSE53819 and GSE64634 datasets of the Gene Expression Omnibus (GEO) database. We mapped receptor operating characteristic profiles for these key PRGs to assess the accuracy of the genes for disease diagnosis and prediction of patient prognosis. In addition, we constructed a nomogram based on these key PRGs and carried out a decision curve analysis. The NPC patients were classified into different pyroptosis gene clusters by the consensus clustering method based on key PRGs, whereas the expression profiles of the key PRGs were analyzed by applying principal component analysis. We also analyzed the differences in key PRGs, immune cell infiltration and NPC‐related genes between the clusters. Finally, we performed differential expression analysis for pyroptosis clusters and obtained differentially expressed genes (DEGs) and performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses.ResultsWe obtained 14 differentially expressed PRGs from GEO database. Based on these 14 differentially expressed PRGs, we applied least absolute shrinkage and selection operator analysis and the random forest algorithm to obtain four key PRGs (CHMP7, IL1A, TP63 and GSDMB). We completely distinguished the NPC patients into two pyroptosis gene clusters (pyroptosis clusters A and B) based on four key PRGs. Furthermore, we determined the immune cell abundance of each NPC sample, estimated the association between the four PRGs and immune cells, and determined the difference in immune cell infiltration between the two pyroptosis gene clusters. Finally, we obtained and functional enrichment analyses 259 DEGs by differential expression analysis for both pyroptosis clusters.ConclusionsPRGs are critical in the development of NPC, and our research on the pyroptosis gene cluster may help direct future NPC therapeutic approaches.
Cu‐catalyzed dehydrogenative CO arylation for the synthesis of 6‐methyl benzofuro[3,2‐c] quinoline derivatives
Authors: Makwana Bhavik S. , Morja Mayur I. , Chauhan Prakashsingh M. , Chikhalia Kishor H. 
Published: January 2024

AbstractA novel strategy for the synthesis of 6‐methyl benzofuro[3,2‐c] quinoline derivatives via copper‐catalyzed dehydrogenative CO arylation has been presented. Optimization studies have been carried out by varying various catalysts, bases, solvents, and other physical parameters. Keeping use of this dehydrogenative cross‐coupling CO arylation reaction, a variety of bioactive building blocks like fused benzofuro quinoline heterocycles were smoothly assembled in moderate to higher yields.
Late MIS5a in the southern North Sea: new chronostratigraphic insights from the Brown Bank Formation
Authors: Waajen Irene M. , Busschers Freek S. , Donders Timme H. , Van Heteren Sytze , Plets Ruth , Wallinga Jakob , Hennekam Rick , Reichart Gert‐Jan , Kinnaird Tim , Wagner‐Cremer Friederike 
Published: January 2024

ABSTRACTThe Brown Bank Formation (BB Fm) is a clay‐rich sedimentary unit that is distributed over a large area in the southern North Sea. It is easily recognisable in 2D acoustic reflection profiles, forming sets of subparallel high‐amplitude reflections. Previous studies have provided only fragmentary information on the facies, and a variety of interpretations on the depositional environment and age of the BB Fm. In this study we combine high‐resolution 2D acoustic reflection profiles and multiproxy sedimentological, geochemical and geochronological analyses of three vibrocores, to provide a detailed description and a new age assessment for the BB Fm at its type locality, adjacent to the Brown Bank ridge. At the study site, the BB Fm shows morphologically well‐preserved submarine sand dunes, deposited in a high‐energy shelf setting, overlain by organic‐rich silty clays that were deposited under lower‐energy conditions in an increasingly compartmentalised marginal marine environment. Elevated terrestrial organic input shows that the Rhine–Meuse delta was situated relatively close‐by. Luminescence ages indicate that the sediments of the BB Fm in our cores were deposited during the Weichselian Odderade interstadial (MIS5a, ca. 80 ka) and earliest parts of the Early Pleniglacial (MIS4, ca. 70 ka), which is later than previously reported in the literature.
Morphologic Analysis of the Scala Tympani Using Synchrotron: Implications for Cochlear Implantation
Authors: Micuda Ashley , Li Hao , Rask‐Andersen Helge , Ladak Hanif M. , Agrawal Sumit K. 
Published: January 2024

ObjectivesTo use synchrotron radiation phase‐contrast imaging (SR‐PCI) to visualize and measure the morphology of the entire cochlear scala tympani (ST) and assess cochlear implant (CI) electrode trajectories.MethodsSR‐PCI images were used to obtain geometric measurements of the cochlear scalar diameter and area at 5‐degree increments in 35 unimplanted and three implanted fixed human cadaveric cochleae.ResultsThe cross‐sectional diameter and area of the cochlea were found to decrease from the base to the apex. This study represents a wide variability in cochlear morphology and suggests that even in the smallest cochlea, the ST can accommodate a 0.4 mm diameter electrode up to 720°. Additionally, all lateral wall array trajectories were within the anatomically accommodating insertion zone.ConclusionThis is the first study to use SR‐PCI to visualize and quantify the entire ST morphology, from the round window to the apical tip, and assess the post‐operative trajectory of electrodes. These high‐resolution anatomical measurements can be used to inform the angular insertion depth that can be accommodated in CI patients, accounting for anatomical variability.Level of evidenceN/A. Laryngoscope, 134:2889–2897, 2024
Morphological characteristics and geometric prediction model of pits in complex corrosion environment
Authors: Chen Yajun , Ma Tianyi , Song Xianjie , Sui Zehuan , Song Xiaoxiao , Zhang XueYang 
Published: January 2024

AbstractFor aircraft serving in coastal environments, the aluminum alloy skin is exposed to high temperature, humidity, and salt environment for a long time. Pitting and exfoliation will affect its normal service life. In this article, static salt spray experiments and electrochemical tests were carried out on 2198‐T8 Al–Li alloy in nine different corrosive environments. The effects of salt concentration, temperature, and humidity on the corrosion behavior were analyzed and compared. The relationship between corrosion environment parameters and pits was established. The results show that, under the same corrosion environment, the length, width, and depth of the pits all follow the log‐normal distribution; compared with the salt concentration and humidity, the increase in temperature has the most obvious promotion on the corrosion rate. Based on the frequency statistics of pit size and electrochemical test results, the pit morphology was simplified to a semiellipsoid geometric model, and the pit size in different corrosive environments was predicted. The predicted size data all fall within the 1.5‐fold dispersion region.
Demonstration of magnetic resonance Z‐spectral imaging for fatty acid characterization of bone marrow at 3 T
Authors: Kuang Junfeng , Qi Yulong , Wu Qiting , Cheng Guanxun , Wu Yin 
Published: January 2024

AbstractMagnetic resonance Z‐spectral imaging (ZSI) has emerged as a new approach to measure fat fraction (FF). However, its feasibility for fat spectral imaging remains to be elucidated. In this study, a single‐slice ZSI sequence dedicated to fat spectral imaging was designed, and its capability for fatty acid characterization was investigated on peanut oil samples, a multiple‐vial fat‐water phantom with varied oil volumes, and vertebral body marrow in healthy volunteers and osteoporosis patients at 3 T. The peanut oil spectrum was also recorded with a 400‐MHz NMR spectrometer. A Gaussian–Lorentzian sum model was used to resolve water and six fat signals of the pure oil sample or four fat signals of the fat‐water phantom or vertebral bone marrow from Z spectra. Fat peak amplitudes were normalized to the total peak amplitude of water and all fat signals. Normalized fat peak amplitudes and FF were quantified and compared among vials of the fat‐water phantom or between healthy volunteers and osteoporosis patients. An unpaired student's t‐test and Pearson's correlation were conducted, with p less than 0.05 considered statistically significant. The results showed that the peanut oil spectra measured with the ZSI technique were in line with respective NMR spectra, with amplitudes of the six fat signal peaks significantly correlated between the two methods (y = x + 0.001, r = 0.996, p < 0.001 under a repetition time of 1.6 s; and y = 1.026x ‐ 0.003, r = 0.996, p < 0.001 under a repetition time of 3.1 s). Moreover, ZSI‐measured FF exhibited a significant correlation with prepared oil volumes (y = 0.876x + 1.290, r = 0.996, p < 0.001). The osteoporosis patients showed significantly higher normalized fat peak amplitudes and FF in the L4 vertebral body marrow than the healthy volunteers (all p < 0.01). In summary, the designed ZSI sequence is feasible for fatty acid characterization, and has the potential to facilitate the diagnosis and evaluation of diseases associated with fat alterations at 3 T.
Secure state estimation via robust optimization for nonlinear cyber‐physical systems
Authors: Chen Lexin , Li Yongming , Tong Shaocheng 
Published: January 2024

AbstractThis article proposes secure state estimation for cyber‐physical systems against sensor attacks. The attack and defense strategies are established via additional historical data, and the defender aims to reduce the estimation error maximally while the attacker aims to degrade the system performance maximally. The algorithm is implemented in the Nash equilibrium framework where the defender first designs the defense strategy and then the attacker designs corresponding attack parameters to launch attacks. Then, a robust optimization problem is formulated using Wasserstein ambiguity sets, which turn out to be equivalent to a convex program. A novel secure observer is proposed, where the attack estimation is used to mitigate attacks. Moreover, the detector is to monitor system behavior and detects the existence of sensor attacks. Finally, simulation results and comparative results illustrate the effectiveness of the defense strategy.
Exteriorization of the uterus reduces fetoscopic cannula‐induced stress and strain: A finite element model analysis
Authors: Ahmad Mirza A. , Vergote Simen , Vander Poorten Emmanuel , Devlieger Roland , De Coppi Paolo , Mazza Edoardo , Deprest Jan 
Published: January 2024

AbstractObjectiveTo estimate stresses and strains in the uterine wall and fetal membranes with single/multi‐port fetoscopy, simulating either a percutaneous access or via exteriorized uterus.Study DesignFinite element models based on anatomical dimensions, material properties and boundary conditions were created to simulate stresses, strains and displacements on the uterine wall and fetal membranes during simulated fetal surgery either via exteriorized uterus or percutaneous approach, and with one or three cannulas. Clinically, we measured the anatomical layer thickness and cannula entry point displacement in patients undergoing single port percutaneous fetoscopy.ResultsSimulations demonstrate that single port percutaneous fetoscopy increases stress on the fetal membranes (+105%, 128 to 262 kPa) and uterine wall (+115%, 0.89 to 1.9 kPa) compared to exteriorized uterine access. Using three ports increases stress by 110% (148 to 312 kPa) on membranes and 113% (1.08 to 2.3 kPa) on uterine wall. Finite Element Method showed 0.75 cm uterine entry point displacement from the cutaneous entry, while clinical measurements demonstrated displacement of more than double (1.69 ± 0.58 cm), suggesting modeled measurements may be underestimations.ConclusionThe stresses and strains on the fetal membranes and uterus are double as high when entering percutaneously than via an exteriorized uterus. Based on what can be clinically measured, this may be an underestimation.
Enhanced performance of hollow polystyrene spheres for inertial confined fusion by noncovalent interactions
Authors: Liu Meifang , Chen Qiang , Liu Yiyang , Li Jie , Xu Wenting , He Zhibing 
Published: January 2024

AbstractThin‐walled polystyrene (PS) spheres are needed for preparing the targets in the inertial confined fusion (ICF). To prevent the occurrence of craze and crack in the fabrication of the spheres, a noncovalent interaction route is developed to improve the mechanical properties of thin‐walled polystyrene spheres based on a fullerene derivative, reducing the craze and crack yield. The introduction of phenyl‐C61‐butyric acid methyl ester (PCBM) into PS spheres not only improves the thermal stability, but also reduces the craze and crack yield, probably to the π–π interaction between PCBM and PS and the hydrophobicity of PCBM. The sphericity and surface finishing of the spheres also increase at the same time. Mechanism of how the PCBM improve the quality of the spheres is also discussed.Highlights
A noncovalent interaction route to improve properties of PS spheres is proposed.
PS/PCBM spheres with good quality are successfully designed and fabricated.
Mechanisms of how the PCBM improve properties of PS spheres are also discussed.
Microsporogenesis and the biosynthesis of floral small interfering RNAs in coffee have a unique pattern among eudicots, suggesting a sensitivity to climate changes
Authors: de Oliveira Kellen Kauanne Pimenta , de Oliveira Raphael Ricon , de Campos Rume Gabriel , Ribeiro Thales Henrique Cherubino , Fernandes‐Brum Christiane Noronha , do Amaral Laurence Rodrigues , Kakrana Atul , Mathioni Sandra , Meyers Blake C. , de Souza Gomes Matheus , Chalfun‐Junior Antonio 
Published: January 2024

AbstractRecently, the siRNAs pathways, and especially reproductive phasiRNAs, have attracted attention in eudicots since their biological roles are still unknown and their biogenesis took different evolutionary pathways compared to monocots. In this work, we used Coffea arabica L., a recent allotetraploid formed from the hybridization of Coffea canephora and C. eugenioides unreduced gametes, to explore microsporogenesis and small RNAs‐related pathways in a eudicot crop. First, we identified the microsporogenesis stages during anther development revealing that pre‐meiosis occurs in anthers of 1.5 mm inside floral buds (FBs), whereas meiosis between 1.5 and 4.2 mm FBs, and post‐meiosis in FBs larger than 4.2 mm. These stages coincide with the Brazilian winter, a period of FBs reduced growth which suggests temperature sensitivity. Next, we identified and quantified the expression of reproductive 21‐ and 24‐nt phasiRNAs during coffee anther development together with their canonical and novel miRNA triggers, and characterized the DCL and AGO families. Our results showed that the pattern of reproductive phasiRNA abundance in C. arabica is unique among described eudicots and the canonical trigger car‐miR2275 is involved in the processing of both 21‐ and 24‐nt phasiRNAs. Fourteen DCL genes were identified, but DCL5, related to phasiRNA biosynthesis in monocots, was not, according to its specificity for monocots. Thus, our work explored the knowledge gap about microsporogenesis and related siRNAs pathways in coffee, contributing to the control of reproductive development and the improvement of fertility in eudicots.
Registration of sorghum backcross‐nested association mapping (BC‐NAM) families in a BTx623 or RTx436 background
Authors: Patil Nikhil Y. , Hoffmann Leo , Winans Noah , Perumal Ramasamy , Hayes Chad , Emendack Yves , Boyles Richard E. , Dahlberg Jeff , Klein Robert R. , Klein Patricia E. , Rooney William L. 
Published: January 2024

AbstractTwo sorghum [Sorghum bicolor (L.) Moench] backcross‐nested association mapping (BC‐NAM) populations (Reg. no. MP‐5, NSL 546724 MAP) have been developed, composed of 30 families totaling 2189 BC1F4 lines using an elite maintainer (B) or restorer (R) line as the recurrent parent. The families were derived from unadapted founder lines that were backcrossed to BTx623 and/or RTx436 based on the fertility reaction score and phylogenetic analysis of the founder lines. These unadapted founder lines were selected based on agronomic fitness, breeder desirability, and genetic diversity and represent a range of races and working groups. The 30 families were developed from 27 founder parents—three of the founder parents were backcrossed to both recurrent parents to provide a genetic relationship across all 30 families. Each family consists of 45–100 BC1F4 lines selected for standard agronomic fitness, and each population was genotyped using genotype‐by‐sequencing. To identify families that possess (or segregate for) traits of interest, 13 lines from each population were randomly selected and phenotyped for a range of agronomic and stress tolerance traits. These BC‐NAM populations, which are adapted to US production regions and possess novel genetic diversity, have application in genetic research as a mapping resource for traits of interest and for sorghum breeding programs to diversify R and B line parental heterotic pools.
Registration of ‘S16‐16641R’: A glyphosate‐tolerant, high‐oleic soybean cultivar with multiple disease resistance
Authors: Chen Pengyin , Shannon J. Grover , Vieira Caio Canella , Lee Dongho , do Nascimento Emanuel Ferrari , Hunt Destiny , Lee Yi‐Chen , Ali Md Liakat , Crisel Melissa , Smothers Scotty , Clubb Michael , Mitchum Melissa Goellner , Meinhardt Clinton G. , Usovsky Mariola 
Published: January 2024

AbstractTo meet the growing demand for high‐oleic soybean [Glycine max (L.) Merr.] cultivars, the University of Missouri‐Fisher Delta Research, Extension, and Education Center soybean breeding program has developed and released ‘S16‐16641R’ (Reg. no. CV‐559, PI 703070). It is a productive maturity group 4‐late (relative maturity 4.8), glyphosate‐tolerant (Roundup Ready 1) cultivar with high‐oleic content (81.7%) and reduced linolenic acid content (4.3%). In addition to its high‐yield potential, S16‐16641R has a broad disease resistance including soybean cyst nematode (Heterodera glycines Ichinohe) races 1 (HG Type 2.5.7), 2 (HG Type 1.2.5.7), 3 (HG Type 5.7), and 5 (HG Type 2.5.7), southern root‐knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood], reniform nematode (Rotylenchulus reniformis Linford & Oliveira), and stem canker (caused by Diaporthe aspalathi Jansen, Castlebury & Crous). It is a chloride excluder with tolerance to high salt field conditions. It was tested in 43 environments across eight states from 2017 to 2020, with an overall relative yield of 99.9% of the mean of the check. The high‐oleic acid content and comprehensive disease resistance combined with broad adaptability and high yield potential across multiple environments make S16‐16641R a promising soybean cultivar for growers to potentially maximize profits by receiving a premium price for high‐oleic oil content.