bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two‐dimensional molecular profiling of mantle cell lymphoma
Authors: Antonucci Francesca , Chilosi Marco , Parolini Claudia , Hamdan Mahmoud , Astner Hubert , Righetti Pier Giorgio 
Published: July 2003

AbstractThe present research establishes standard two‐dimensional (2‐D) maps for control, reactive lymph node and non‐Hodgkin's lymphoma (mantle cell lymphoma, MCL). Medium sensitivity, mass spectrometry compatible colloidal Coomassie has revealed a total of ca. 750 spots in each of the maps. Comparison of 2‐D maps by statistical packages, such as the PDQuest, established up‐ and downregulation of a total of ca. 145 spots, with positive variations of up to 10‐folds and negative variations of up to 13‐folds in both MCL biopsies' protein extracts. Qualitative and quantitative variations in the two lymphoma samples are consistent. More than 20 proteins have been so far identified by matrix assisted laser desorption/ionisation‐time of flight (MALDI‐TOF)‐mass spectrometry, with an additional five spots, which gave very good spectra but could not be matched to any of the presently available databases. Some of the spots, such as the 78 kDa glucose‐regulated protein precursor and the glutathione S‐transferase P, appear to be in common with other tumors, such as lung adenocarcinoma. Others may simply reflect overall changes in cellular metabolism and growth rate that occur during malignancy and thus might turn out to be in common with any cell population receiving any kind of stress. Some (notably T‐cell leukemia/lymphoma protein 1A, TCL1, found to be 10‐fold overexpressed) appear to be specific of the non‐Hodgkin's lymphoma here studied. Western blot and immunohistochemical analyses were applied to obtain further information about stathmin (Op18) and TCL1, respectively.
Expression profiling of 68 glycosyltransferase genes in 27 different human tissues by the systematic multiplex reverse transcription‐polymerase chain reaction method revealed clustering of sexually related tissues in hierarchical clustering algorithm analysis
Authors: Yamamoto Miyako , Yamamoto Fumiya , Luong Trang T. , Williams Tristan , Kominato Yoshihiko , Yamamoto Fumiichiro 
Published: July 2003

AbstractWe have developed an experimental system to study the expression of 68 human glycosyltransferase genes. Using this system, we examined the expression of those genes in 27 different tissues by the technique which we named systematic multiplex reverse transcription‐polymerase chain reaction (SM RT‐PCR). The panoramic view of a total of 1836 (68×27) expression data demonstrates that some glycosyltransferase genes are differentially expressed whereas some others are ubiquitously expressed. The data gathered provide more information on glycosyltransferase gene expression in tissues than any other paper published, and surpass in quantity all the information combined from previous publications. Although the expression profiling of glycosyltransferase genes alone may not directly explain the repertoires of oligosaccharides synthesized, it is an important step toward a better understanding of the gene expression network involved in oligosaccharide synthesis/degradation. Our modestly high‐throughput gene expression study and the data analysis using a hierarchical clustering algorithm have allowed us to investigate the correlation between tissues and glycosyltransferase gene expression. Similar patterns of glycosyltransferase gene expression were observed in functionally and anatomically related tissues. All, but one, sexually related tissues formed a cluster in a tissue dendrogram, suggesting the involvement of sex hormones in the transcriptional control of many glycosyltransferase genes. Once established, the SM RT‐PCR is cost‐ and time‐efficient and requires small amounts of RNA as template. It is especially useful for the simultaneous analyses of multiple samples. Because of its simple design, the SM RT‐PCR may offer an easy alternative in studying the expression of many other families of genes, as well as groups of related/unrelated genes, in various biological phenomena.
Determination of bupivacaine and metabolites in rat urine using capillary electrophoresis with mass spectrometric detection
Authors: Krisko Ryan M. , Schieferecke Mark A. , Williams Todd D. , Lunte Craig E. 
Published: July 2003

AbstractA method using capillary electrophoresis‐mass spectrometry (CE‐MS) was developed for the structural elucidation of bupivacaine and metabolites in rat urine. Prior to CE‐MS analysis, solid‐phase extraction (SPE) was used for sample cleanup and preconcentration purposes. Exact mass and tandem mass spectrometric (MS/MS) experiments were performed to obtain structural information about the unknown metabolites. Two instruments with different mass analyzers were used for mass spectrometric detection. A quadrupole time‐of‐flight (Q‐TOF) and a magnetic sector hybrid instrument were coupled to CE and used for the analysis of urine extracts. Hydroxybupivacaine as well as five other isomerically different metabolites were detected including methoxylated bupivacaine.
Ultrathin‐layer sodium dodecyl sulfate disc electrophoresis of proteins in the range from 10 to 220 kDa in homogeneous, low‐concentrated polyacrylamide gels
Authors: Maly I. Piotr , Crotet Valérie , Toranelli Mireille 
Published: July 2003

AbstractThis study describes an ultrathin‐layer sodium dodecyl sulfate (SDS) disc electrophoresis in polyacrylamide gels of a thickness of only 150 μm. By use of 2‐amino‐2‐methyl‐1,3‐propanediol/glycine instead of traditional Tris/HCl buffer in the resolving phase of the gel, proteins with a wide range of molecular sizes (10 kDa to over 220 kDa) are separated in unusually low‐concentrated gels (4%T, 3.3%C). 2‐Amino‐2‐methyl‐1,3‐propanediol in the resolving part of the gel contributes to stabilization of the pH value at 8.8, while glycine improves destacking as well as separation of small proteins from the bulk of stacked SDS. This method combines both the advantages of conventional slab‐gel electrophoresis and capillary gel electrophoresis. It is easy to apply and well suited for all further miniaturization attempts.
Carrier ampholyte‐free solution isoelectric focusing as a prefractionation method for the proteomic analysis of complex protein mixtures
Authors: Shang Tanya Q. , Ginter Joy M. , Johnston Murray V. , Larsen Barbara S. , McEwen Charles N. 
Published: July 2003

AbstractThe field of proteomics requires methods that offer high sensitivity and wide dynamic range. One of the strategies used to improve the dynamic range is sample prefractionation, such as microsolution isoelectric focusing (IEF). We have modified a commercial solution IEF instrument, the Rotofor®, to prefractionate protein mixtures by carrier ampholyte‐free solution IEF. The focusing chamber of the Rotofor was divided into several compartments by polyacrylamide membranes with imbedded Immobiline mixtures of specific pH values. When an electric field is applied, each protein migrates to the compartment confined by membranes with pH values flanking its isoelectric point. The approach was demonstrated for the focusing of myoglobin into a predicted compartment, as well as the separation of a complex soluble yeast protein mixture into several distinct fractions. The proteins were dissolved in water or 30% isopropanol. The method is applicable to both gel‐based and solution‐phase protein identification methods, without the need for further sample preparation.
Exposure to chronic, low‐dose rate γ‐radiation at Chornobyl does not induce point mutations in Big Blue® mice
Authors: Wickliffe Jeffrey K. , Bickham Amy M. , Rodgers Brenda E. , Chesser Ronald K. , Phillips Carleton J. , Gaschak Sergey P. , Goryanaya J.A. , Chizhevsky I. , Baker Robert J. 
Published: July 2003

AbstractEmpirical genetic effects resulting from low‐dose rate irradiation and chronic, cumulative exposure are poorly characterized. Expected effects are based on epidemiological studies and downward, linear extrapolations from nonthreshold models derived from acute, high‐dose exposures. These extrapolations and their associated risk coefficients have no experimental support, and because of their inherent uncertainty they are the subject of considerable debate. The expectation of deleterious genetic effects resulting from low‐dose rate irradiation and chronic exposure is in need of empirical assessment because this type of exposure is typical of those encountered in occupational, residential, and environmental settings. Recent acute low‐dose (<10 cGy) studies using cytogenetic and point mutation endpoints indicate that observed effects range from those lower than spontaneous to an increase in the frequency of point mutations. Using the Big Blue® assay, we examined the ability of chronic, continuous γ‐irradiation (2.3 × 10‐3 cGy/min) in the Chornobyl environment to induce point mutations. This system has demonstrated a significant point mutation sensitivity (4.5‐fold increase) to acute, high‐dose (1–3 Gy) γ‐radiation. Mutant frequencies and the mutation spectra were examined in exposed and reference samples of Big Blue® mice following 90 days exposure (cumulative absorbed dose = 3 Gy) to the Chornobyl environment. No significant increase in the mutant frequency or bias in the mutational spectrum was observed in exposed individuals. This finding suggests that low‐dose rate γ‐irradiation at Chornobyl does not induce point mutations and that cumulative, chronically absorbed doses do not induce the same genetic effects as acute doses of the same magnitude. Environ. Mol. Mutagen. 42:11–18, 2003. © 2003 Wiley‐Liss, Inc.
Comparative analysis of HPRT mutant frequency in children with cancer
Authors: Rice Sederick C. , Vacek Pamela M. , Homans Alan H. , Kendall Heather , Rivers Jami , Messier Terri , Finette Barry A. 
Published: July 2003

AbstractThe link between exposure to environmental mutagens and the development of cancer is well established. Yet there is a paucity of data on the relationship between gene–environment interactions and the mechanisms associated with the somatic mutational events involved with malignant transformation, especially in children. To gain insight into somatic mutational mechanisms in children who develop cancer, we determined the background mutant frequency (Mf) in the hypoxanthine phosphoribosyl transferase (HPRT) reporter gene of peripheral blood lymphocytes from pediatric cancer patients at the time of diagnosis and prior to therapeutic intervention. We studied 23 children with hematologic malignancies and 31 children with solid tumors prior to initial therapeutic intervention. Children with solid tumors, specifically sarcomas, and Hodgkin's disease were significantly older and had elevated HPRT Mfs (6.1 × 10−6 and 3.7 × 10−6, respectively) at the time of diagnosis, compared to normal controls (2.3 × 10−6) and other pediatric tumor groups including children with acute lymphocytic leukemia and non‐Hodgkin's lymphoma (ALL/NHL, 1.7 × 10−6), central nervous system tumors (CNS, 3.6 × 10−6), and neuroblastoma (1.9 × 10−6). Of importance is that the significant differences observed in HPRT Mfs between these groups no longer existed after correcting for the effects of age. These data demonstrate that in children who develop cancer there appears to be no significant increase in background HPRT Mf that would indicate significant exposure to genotoxic chemicals or an underlying DNA repair defect resulting in genomic instability. In addition, these data demonstrate the importance of correcting for the effect of age when comparing the frequency of somatic mutations in children and should provide baseline data for future longitudinal biomonitoring studies on the genetic effects of chemotherapy in children treated for cancer. Environ. Mol. Mutagen. 42:44–49, 2003. © 2003 Wiley‐Liss, Inc.
Gene expression patterns predict exposure to PCBs in developing Xenopus laevis tadpoles
Authors: Jelaso Anna M. , Lehigh‐Shirey Elisabeth , Means Jay , Ide Charles F. 
Published: July 2003

AbstractPolychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants that pose global ecological and human health problems. Although it is well established that PCBs are associated with a variety of adverse health effects in wildlife and in humans, it is often difficult to determine direct cause‐and‐effect relationships between exposure and specific health outcomes. In this study, gene expression signatures were used to relate exposure to PCBs with altered physiological responses and/or specific health effects. Real‐time PCR was used to measure gene expression levels for 10 genes in Xenopus laevis tadpoles (18 days postfertilization, PF) after acute exposure (2 days) to the PCB mixture Aroclor 1254. Specific gene expression signatures correlated with exposure and were predictive of adverse health effects. Exposure to low levels of Aroclor 1254 (5–50 ppb) significantly increased expression of six genes, independent of any health effects; exposure to midlevel concentrations (300–400 ppb) significantly decreased expression levels of two genes, NGF and β‐actin, prior to the onset of observable health effects; exposure to higher doses (500–700 ppb) significantly decreased NGF and β‐actin expression concomitant with the appearance of gross morphological abnormalities, behavioral deficits, and a statistically significant decrease in survival. This study expands upon our previous work that demonstrated an age‐dependent susceptibility to Aroclor 1254 in Xenopus laevis tadpoles and that defined specific gene expression signatures as useful bioindicators of exposure and as predictors of overt or impending health effects. Environ. Mol. Mutagen. 42:1–10, 2003. © 2003 Wiley‐Liss, Inc.
Developing new conservation partnerships: Expanding opportunities for integrating resource stewardship and mission requirements
Authors: Boice L. Peter 
Published: July 2003

AbstractThe Department of Defense's (DOD) conservation program has long used partnerships with federal
and state agencies, conservation organizations, and other groups to promote resource stewardship, increase public
awareness and appropriate use of resources, improve quality of life, encourage volunteer opportunities, and
improve management efficiencies. This article describes some of DOD's most successful conservation
partnerships; explores what makes a good partnership; summarizes the benefits of these
partnerships; and suggests some possible trends in future conservation partnerships. © 2003 Wiley
Periodicals, Inc.
Deciding how clean is clean enough under the Texas Law of risk‐based corrective action
Authors: Pepper Christopher B. , Block Nathan , Baladi Sadika 
Published: July 2003

AbstractUnder the Texas Risk Reduction Program (TRRP), the principles of risk‐based corrective action
(RBCA) are applied when determining the appropriate cleanup standards for contaminated sites. RBCA is
premised upon the belief that scarce resources are better allocated on other necessities when the budgets for
corrective action merely provide for negligible or marginal benefits. Therefore, RBCA seeks to establish cleanup
levels based on the actual risk to human and environmental health. Although a valiant undertaking, such regulations
are relatively new and may not provide adequate protection for the environment, current landowners, and/or
subsequent purchasers. Limitations associated with RBCA include the various uncertainties and complexities inherent
with all risk assessment paradigms, as well as the potential for conflicts with regulatory laws. Understanding RBCA,
as applied in TRPP, may be important in resolving future liability issues, especially those associated with toxic
tort litigation. © 2003 Wiley Periodicals, Inc.
Environmental management systems (EMS) implementation at chemical demilitarization facilities
Authors: Wood James D. , Baetz Linda L. , Shaheen Charles A. 
Published: July 2003

AbstractAdopting the Department of the Army (DA) “installation” EMS policy guidance to a key
DA Acquisition Program, the Program Manager for Chemical Demilitarization (PMCD), in conjunction with
the U.S. Army Center for Health Promotion and Preventive Medicine (USACHPPM) undertook an initiative
to use the ISO 14001 framework for the EMS at chemical demilitarization facilities. The Pine Bluff Chemical
Agent Disposal Facility (PBCDF) was selected as the pilot facility for this EMS initiative. This
paper presents a case study of the EMS implementation at the PBCDF; PMCD remote monitoring of the PBCDF
EMS implementation effort using the Equation ASP software; and external ISO 14001 conformance validation
of the PBCDF EMS by USACHPPM's Registrar Accreditation Board (RAB)‐certified EMS
auditors. © 2003 Wiley Periodicals, Inc.
Small arms range lead management issues: Activated carbon, molecular sieves (5A and 13X), and naturally occurring zeolites as lead adsorbents
Authors: Abdel‐Fattah Tarek M. , Isaacs Larry K. , Payne Kelly B. 
Published: July 2003

AbstractLead in storm water runoff from small arms ranges (SAR) at Barksdale AFB, LA, has exceeded Louisiana
Pollution Discharge Elimination System (LPDES) limits. Range managers need cost‐effective ways to
manage soluble, and clay colloid adsorbed, lead species. Two steps were suggested and undertaken to try to control
soluble lead species. First, the soluble lead species were immobilized by adding phosphorous pentoxide
(P2O5) to the top soil and mixing it in. Before treatment the combined range mean
total lead was 865 μg l−1 from Jun 2001 to Mar 2002 at a pH = 7.1. After treatment the
M‐16 SAR mean total lead from Oct 25 to Dec 5, 2002, in the soil being tested, was 59 μg
l−1 at a pH = 7.4. Second, low‐cost adsorbents were investigated to
“capture” lead ions in storm water before discharge to a receiving stream. Therefore, batch adsorption
and kinetic studies were conducted to compare and evaluate different types of adsorbents for lead removal from
aqueous media. Adsorbent materials such as activated carbon and naturally occurring zeolites (clinoptilolite
and chabazite) are potential point‐of‐use materials for mitigating waste water runoff.
Molecular sieves (13X and 5A) were selected because they provide a basis for comparison with previous
studies and represent well‐characterized materials. The relative rate for lead adsorption was: 13X >
chabazite > clinoptilolite > 5A > activated carbon. This article describes how environmental managers
researched and identified a corrective action and developed a maintenance plan to bring the installation SAR into
regulatory compliance. © 2003 Wiley Periodicals, Inc.
Composition of the essential oil of Stachys setifera C. A. Mey ssp. iranica growing in Iran
Authors: Javidnia K. , Miri R. , Azarpira A. , Tabaei S. M. H. 
Published: July 2003

AbstractThe essential oil composition of Stachys setifera ssp. iranica has been investigated by GC and GC–MS; 73 compounds, representing 96.7% of the oil were identiﬁed, of which pulegone (26.5%), piperitenone oxide (17.4%) and α‐terpinyl acetate (11.2%) were the main components. Copyright © 2003 John Wiley & Sons, Ltd.
Analysis of the essential oil of Stachys ixodes Boiss. & Hausskn. ex Boiss. from Iran
Authors: Seﬁdkon F. , Naderi Nik A. , Bagaii P. , Javidtash I. 
Published: July 2003

AbstractHydrodistilled volatile oil from the aerial parts of Stachys ixodes Boiss. & Hausskn. ex Boiss. was analysed by a combination of GC and GC–MS. 20 components were identiﬁed, constituting approximately 95.7% of the oil. The main constituents of the essential oil were myrtenyl acetate (48.7%), globulol (13.1%), caryophyllene oxide (5.9%) and spathulenol (5.6%). Copyright © 2003 John Wiley & Sons, Ltd.
Synthesis and odour evaluation of stereoisomers of N‐Alkyl‐pyrrolidine derivatives, characteristic roast odour of seafood
Authors: Tachihara Toru , Ishizaki Susumu , Kurobayashi Yoshiko , Tamura Hiroshi , Ikemoto Youichi , Onuma Atsushi , Kitahara Takeshi 
Published: July 2003

AbstractAll the possible stereoisomers of N‐(2′‐methylbutyl)pyrrolidine 1 and N‐(2′‐methylbutyl)‐2‐methylpyrrolidine 2, which have the characteristic roast odour of seafood, were synthesized. As a result of odour evaluation of all the isomers, we found that each isomer has a different and characteristic odour. Copyright © 2003 John Wiley & Sons, Ltd.
The leaf essential oil of Costus afer Ker–Grawl from Nigeria
Authors: Taiwo Asekun O. , Bolanle Adeniyi A. 
Published: July 2003

AbstractDetailed systematic and comprehensive analysis of the essential oil of the leaves of Costus afer Ker–Grawl (Zingiberaceae) from the western part of Nigeria by capillary gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS) led to the identiﬁcation of 27 compounds from 38 compounds in the essential oil. The results showed that sesquiterpenoids were the most abundant group of volatile compounds, with sesquilavandulyl acetate (17.0%) as the principal component. β‐caryophyllene (12.3%) and Z,E‐farnesol (9.9%) were also present in reasonable quantities. Investigation of the antimicrobial activity of the essential oil showed that it is inactive. Copyright © 2003 John Wiley & Sons, Ltd.
Volatile constituents of fruits of Garcinia dulcis Kurz. from Cuba
Authors: Pino Jorge , Marbot Rolando , Rosado Arístides , Vázquez Carlos 
Published: July 2003

AbstractThe volatile constituents of rata fruits (Garcinia dulcis Kurz.) growing in Cuba were analysed by GC–MS. Ninety compounds were identiﬁed in the aroma concentrate, of which linalool, α‐terpineol and hexadecanoic acid were found to be the major constituents. Copyright © 2003 John Wiley & Sons, Ltd.
Characterization of the major odorants found in the peel oil of Citrus reticulata Blanco cv. Clementine using gas chromatography–olfactometry
Authors: Chisholm Mary G. , Jell Jason A. , Cass Dean M. 
Published: July 2003

AbstractGas chromatography–olfactometry (GC–O), gas chromatography–mass spectrometry (GC–MS) and preparative column chromatography were used to identify the key odorants present in laboratory‐extracted clementine oil from Spain. Almost 50 odorants were identiﬁed using GC–O, many of which were unsaturated aldehydes with high odour spectrum values (OSV). α‐ and β‐sinensal, trans‐4,5‐epoxy‐(E)‐2‐decanal, (E,Z)‐2,6‐dodecadienal and linalool were found to dominate clementine oil aroma. Enrichment of the oxygenates using preparative column chromatography provided further identiﬁcation of a total of 50 aldehydes, not all of which were present in the oil at concentrations high enough to produce a response using GC–O. Aldehydes contributed approximately 80% of the total aroma of clementine oil. New odorants not previously reported in clementine oil include many unsaturated aldehydes, trans‐4,5‐epoxy‐(E)‐2‐decenal, trans‐4,5‐epoxy‐(E)‐2‐dodecenal, 4‐hydroxy‐2,5‐dimethyl‐3(2H)‐furanone (furaneol), 3‐hydroxy‐4,5‐dimethyl‐3(2H)‐furanone (sotolon) and 1,8‐cineole. No single odorant emerged as being characteristic of clementine oil aroma. Copyright © 2003 John Wiley & Sons, Ltd.
Isolation and characterization of several aromatic sesquiterpenes from Commiphora myrrha
Authors: Zhu Nanqun , Sheng Shuqun , Sang Shengmin , Rosen Robert T. , Ho Chi‐Tang 
Published: July 2003

AbstractThe puriﬁcation and structure characterization of seven aromatic sesquiterpenes from scented Commiphora myrrha are reported. Their structures were determined on the basis of spectral data, especially of NMR evidence. Among them, compound 1 is a new furanosesquiterpene and compound 2 is identiﬁed as a new natural aroma found in Commiphora spp. All compounds except 4 were isolated, for the ﬁrst time, from Commiphora myrrha. Copyright © 2003 John Wiley & Sons, Ltd.
Chemical constituents of the essential oil of the hornwort Anthoceros caucasicus
Authors: Sonwa Mesmin Mekem , König Wilfried A. 
Published: July 2003

AbstractThe essential oil of the hornwort Anthoceros caucasicus (Bryophytae), collected on the island of Madeira, was investigated by gas chromatography, mass spectrometry and NMR techniques. A new sesquiterpene ether, veticadinoxide (1) was identiﬁed as the major constituent. Copyright © 2003 John Wiley & Sons, Ltd.