bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Printable Hydrogel Loaded with Medicinal Plant Extract for Promoting Wound Healing
Authors: Wang Xiaoyu , Zhang Yue , Song Anning , Wang Heng , Wu Yi , Chang Wenju , Tian Bo , Xu Jialu , Dai Huaxing , Ma Qingle , Wang Chao , Zhou Xiaozhong 
Published: January 2024

AbstractHow to promote wound healing is still a major challenge in the healthcare while macrophages are a critical component of the healing process. Compared to various bioactive drugs, many plants have been reported to facilitate the wound healing process by regulating the immune response of wounds. In this work, a Three‐dimensional (3D) printed hydrogel scaffold loaded with natural Centella asiatica extract (CA extract) is developed for wound healing. This CA@3D scaffold uses gelatin (Gel) and sodium alginate (SA) with CA extract as bio‐ink for 3D printing. The CA extract contains a variety of bioactive compounds that make the various active ingredients in Centella asiatica work in concert. The printed CA@3D scaffold can fit the shape of wound, orchestrate the macrophages and immune responses within the wound, and promote wound healing compared to commercial wound dressings. The underlying mechanism of promoting wound healing is also illuminated by applying multi‐omic analyses. Moreover, the CA extract loaded 3D scaffold also showed great ability to promote wound healing in diabetic chronic wounds. Due to its ease of preparation, low‐cost, biosafety, and therapeutic outcomes, this work proposes an effective strategy for promoting chronic wound healing.
Self‐Assembled Nanocomposite Hydrogels as Carriers for Demineralized Bone Matrix Particles and Enhanced Bone Repair
Authors: Chen Chen , Li Zhi , Xu Changlu , Kang Minjee , Lee Chung‐Sung , Aghaloo Tara , Lee Min 
Published: January 2024

AbstractDemineralized bone matrix (DBM) has been widely used as an allogeneic alternative to autologous bone graft for bone repair. However, more extensive use of DBM is limited due to its particulate nature after demineralization and rapid particle dispersion following irrigation, resulting in unpredictable osteoinductivity. Here, a new design of injectable hydrogel carriers for DBM that combine self‐healing ability and osteogenic properties based on the self‐assembly of guanidinylated hyaluronic acid and silica‐rich nanoclays is reported. The nanoclays serve as reversible linkages to form a dynamic hydrogel network with the guanidine moieties on the polymer chains. Gelation kinetics and mechanical properties can be controlled by altering nanoclay content in the hydrogel. The resulting hydrogel exerts self‐healing ability due to its dynamic crosslinks and well retains its overall performance with high DBM loading. The hydrogel exhibits great cytocompatibility and osteogenic effects mediated by the nanoclays. In vivo delivery of DBM using the nanocomposite hydrogel further demonstrates robust bone regeneration in a mouse calvarial defect model in comparison to DBM delivered with aqueous HA. This work suggests a promising hydrogel platform for many applications including therapeutic delivery and tissue engineering.
Artificial Cells and HepG2 Cells in 3D‐Bioprinted Arrangements
Authors: Westensee Isabella N. , Paffen Lars J.M.M. , Pendlmayr Stefan , De Dios Andres Paula , Ramos Docampo Miguel A. , Städler Brigitte 
Published: January 2024

AbstractArtificial cells are engineered units with cell‐like functions for different purposes including acting as supportive elements for mammalian cells. Artificial cells with minimal liver‐like function are made of alginate and equipped with metalloporphyrins that mimic the enzyme activity of a member of the cytochrome P450 family namely CYP1A2. The artificial cells are employed to enhance the dealkylation activity within 3D bioprinted structures composed of HepG2 cells and these artificial cells. This enhancement is monitored through the conversion of resorufin ethyl ether to resorufin. HepG2 cell aggregates are 3D bioprinted using an alginate/gelatin methacryloyl ink, resulting in the successful proliferation of the HepG2 cells. The composite ink made of an alginate/gelatin liquid phase with an increasing amount of artificial cells is characterized. The CYP1A2‐like activity of artificial cells is preserved over at least 35 days, where 6 nM resorufin is produced in 8 h. Composite inks made of artificial cells and HepG2 cell aggregates in a liquid phase are used for 3D bioprinting. The HepG2 cells proliferate over 35 days, and the structure has boosted CYP1A2 activity. The integration of artificial cells and their living counterparts into larger 3D semi‐synthetic tissues is a step towards exploring bottom‐up synthetic biology in tissue engineering.
Circularly Polarized Organic Light‐Emitting Diodes Based on Chiral Hole Transport Enantiomers
Authors: Zhong Xiao‐Sheng , Yuan Li , Liao Xiang‐Ji , Hu Jia‐Jun , Xing Shuai , Song Shi‐Quan , Xi Jia‐Qi , Zheng You‐Xuan 
Published: January 2024

AbstractThe circularly polarized organic light‐emitting diodes (CP‐OLEDs) demonstrate promising application in 3D display due to the direct generation of circularly polarized electroluminescence (CPEL). But the chiral luminescence materials face challenges as intricated synthetic route, enantiomeric separation, etc. Herein, fresh CP‐OLEDs are designed based on chiral hole transport material instead of chiral emitters. A pair of hole transport enantiomers (R/S‐NPACZ) exhibit intense dissymmetry factors (|gPL|) about 5.0 × 10−3. With R/S‐NPACZ as hole transport layers, CP‐OLEDs are fabricated employing six achiral phosphorescence and thermally activated delayed fluorescence (TADF) materials with different wavelengths, in consistence with the generated CPEL spectra. The CP‐OLEDs based on achiral red, green, and blue iridium(III) complexes exhibit external quantum efficiencies (EQEs) of 14.9%, 30.7%, and 14.1% with |gEL| factors of 8.8 × 10−4, 2.3 × 10−3, and 2.0 × 10−3, respectively. Moreover, the devices using achiral blue, blueish‐green, and green TADF materials display EQEs of 24.1%, 17.9%, and 25.4% with |gEL| factors of 1.0 × 10−3, 3.6 × 10−3, and 2.2 × 10−3, respectively. As far as known, it is the first example of CP‐OLEDs based on chiral hole transport materials, which act as the organic circularly polarizers and have potential to generate CPEL from achiral luminescence materials.
Ln‐MOF‐Based Hydrogel Films with Tunable Luminescence and Afterglow Behavior for Visual Detection of Ofloxacin and Anti‐Counterfeiting Applications
Authors: Yu Xiaolin , Ryadun Alexey A. , Pavlov Dmitry I. , Guselnikova Tatiana Y. , Potapov Andrei S. , Fedin Vladimir P. 
Published: January 2024

AbstractHighly selective and sensitive quantitative detection of ofloxacin (OFX) at ultralow concentrations in aqueous media and development of new afterglow materials remains a challenge. Herein, a new 2D water‐stable lanthanide metal–organic framework (NIIC‐2‐Tb) is proposed, which exhibits high selectivity towards OFX through the luminescence quenching with the lowest detection limit (1.1 × 10−9 M) reported to date and a fast response within 6 s. In addition, the luminescent detection of OFX by NIIC‐2‐Tb is not affected by typical components of blood plasma and urine. The excellent sensing effect of NIIC‐2‐Tb is further utilized to prepare a composite functional sensing carrageenan hydrogel material for the rapid detection of OFX in meat in real time and the first discovery of impressive afterglow in MOF‐based hydrogels. This study not only presents novel Ln‐MOF materials and Ln‐MOF‐based hydrogel films for luminescent sensing of OFX, but also demonstrates color‐tunable luminescent films with afterglow, which expands the application of composite luminescent materials for detection and anti‐counterfeiting.
External Mechanical Stability Regulates Hematoma Vascularization in Bone Healing Rather than Endothelial YAP/TAZ Mechanotransduction
Authors: Mehl Julia , Farahani Saeed Khomeijani , Brauer Erik , Klaus‐Bergmann Alexandra , Thiele Tobias , Ellinghaus Agnes , Bartels‐Klein Eireen , Koch Katharina , Schmidt‐Bleek Katharina , Petersen Ansgar , Gerhardt Holger , Vogel Viola , Duda Georg N. 
Published: January 2024

AbstractBone fracture healing is regulated by mechanobiological cues. Both, extracellular matrix (ECM) deposition and microvascular assembly determine the dynamics of the regenerative processes. Mechanical instability as by inter‐fragmentary shear or compression is known to influence early ECM formation and wound healing. However, it remains unclear how these external cues shape subsequent ECM and microvascular network assembly. As transcriptional coactivators, the mechanotransducers yes‐associated protein 1 (YAP)/transcriptional coactivator with PDZ‐binding motif (TAZ) translate physical cues into downstream signaling events, yet their role in sprouting angiogenesis into the hematoma after injury is unknown. Using bone healing as model system for scar‐free regeneration, the role of endothelial YAP/TAZ in combination with tuning the extrinsic mechanical stability via fracture fixation is investigated. Extrinsically imposed shear across the gap delayed hematoma remodeling and shaped the morphology of early collagen fiber orientations and microvascular networks, suggesting that enhanced shear increased the nutrient exchange in the hematoma. In contrast, endothelial YAP/TAZ deletion has little impact on the overall vascularization of the fracture gap, yet slightly increases the collagen fiber deposition under semi‐rigid fixation. Together, these data provide novel insights into the respective roles of endothelial YAP/TAZ and extrinsic mechanical cues in orchestrating the process of bone regeneration.
Mortality of older construction and craft workers employed at Department of Energy (DOE) nuclear sites: Follow‐up through 2021
Authors: Ringen Knut , Dement John , Cloeren Marianne , Almashat Sammy , Hines Stella , Grier William , Quinn Patricia , Chen Anna , Haas Scott 
Published: January 2024

AbstractBackgroundTo determine if construction and trades workers formerly employed at US Department of Energy (DOE) nuclear weapons sites are at significant risk for occupational diseases, we studied the mortality experience of participants in the Building Trades National Medical Screening Program (BTMed).MethodsThe cohort included 26,922 participants enrolled between 1998 and 2021 and 8367 deaths. Standardized mortality ratios were calculated based on US death rates. Cox models compared construction workers (n = 22,747; 7487 deaths) to two nonconstruction subpopulations: administrative, scientific and security workers (n = 1894; 330 deaths), and all other nonconstruction workers (n = 2218; 550 deaths).ResultsMortality was elevated for all causes, all cancers, cancers of the trachea, bronchus, lung, kidneys, and lymphatic and hematopoietic system, mesothelioma, chronic obstructive pulmonary disease (COPD), asbestosis, transportation injuries, and other injuries, particularly accidental poisonings. There were 167 deaths from coronavirus disease 2019 (COVID‐19), which was lower than expected using US death rates. Overall cause‐specific mortality was significantly higher among construction workers than for internal comparison groups.ConclusionsConstruction workers employed at DOE sites have a significantly increased risk for occupational illnesses. Apart from COVID‐19 deaths, this update: (1) found that mortality among construction workers is significantly elevated compared to the US population and significantly higher than in the internal comparison populations, and (2) confirmed excess risk for these workers for first employment after 1990. Cancer mortality risks are similar to the cancers identified for DOE compensation from radiation exposures. The high lung cancer risk supports the value of early lung cancer detection. Continued medical surveillance is important.
Walking the talk for dementia: A unique immersive, embodied, and multi‐experiential initiative
Authors: Aguzzoli Peres Fernando , Haas Aline Nogueira , Martha Angga Dwi , Chan Melissa , Steele Michelle , Ferretti Maria Teresa , Ngcobo Ntokozo N. , Ilinca Stefania , Domínguez‐Vivero Clara , Leroi Iracema , Sajnani Nisha , Zimmer Eduardo R. , Kornhuber Alex , Kalache Alexandre , Holtzhausen Berrie , Tristão‐Pereira Catarina , Dupont Charlèss , Cohen Dan , de Jong David , Facal David , O'Sullivan Desmond , Mateus Elaine , Roso Enzo , Estrop Evon , Gamba Giulia , San Martin Elexpe Gustavo , Ulises Diaz Hernández Hector , Quaid Helena , Govia Ishtar , Barbosa João , García del Moral Joaquina , Miller Johnny , García García José Antonio , Quaid Kevin , Navarro Laura , García Laura Zúñiga , Waters Laureen , Molete Lebo , Godoy Maria Eugenia , Sigauke Maureen , de Ataide Schulte Max William , Estrop Peter , San Martin Elexpe Cardoso Priscilla , Perez Randall , Patterson Rianna , Chakrabarti Ronika , Wong Ruth , Marsillas Sara , Lowe Shontonese , Rego Taida Sánchez , Farombi Temitope , Montgomery Theresa , in ‘t Veen Ton , Yanni Valli , Weidner Wendy , Ibanez Agustin 
Published: January 2024

AbstractCoping with dementia requires an integrated approach encompassing personal, health, research, and community domains. Here we describe “Walking the Talk for Dementia,” an immersive initiative aimed at empowering people with dementia, enhancing dementia understanding, and inspiring collaborations. This initiative involved 300 participants from 25 nationalities, including people with dementia, care partners, clinicians, policymakers, researchers, and advocates for a 4‐day, 40 km walk through the Camino de Santiago de Compostela, Spain. A 2‐day symposium after the journey provided novel transdisciplinary and horizontal structures, deconstructing traditional hierarchies. The innovation of this initiative lies in its ability to merge a physical experience with knowledge exchange for diversifying individuals' understanding of dementia. It showcases the transformative potential of an immersive, embodied, and multi‐experiential approach to address the complexities of dementia collaboratively. The initiative offers a scalable model to enhance understanding, decrease stigma, and promote more comprehensive and empathetic dementia care and research.
C5aR1 signaling promotes region‐ and age‐dependent synaptic pruning in models of Alzheimer's disease
Authors: Gomez‐Arboledas Angela , Fonseca Maria I. , Kramar Enikö , Chu Shu‐Hui , Schartz Nicole D. , Selvan Purnika , Wood Marcelo A. , Tenner Andrea J. 
Published: January 2024

AbstractINTRODUCTIONSynaptic loss is a hallmark of Alzheimer's disease (AD) that correlates with cognitive decline in AD patients. Complement‐mediated synaptic pruning has been associated with this excessive loss of synapses in AD. Here, we investigated the effect of C5aR1 inhibition on microglial and astroglial synaptic pruning in two mouse models of AD.METHODSA combination of super‐resolution and confocal and tridimensional image reconstruction was used to assess the effect of genetic ablation or pharmacological inhibition of C5aR1 on the Arctic48 and Tg2576 models of AD.RESULTSGenetic ablation or pharmacological inhibition of C5aR1 partially rescues excessive pre‐synaptic pruning and synaptic loss in an age and region‐dependent fashion in two mouse models of AD, which correlates with improved long‐term potentiation (LTP).DISCUSSIONReduction of excessive synaptic pruning is an additional beneficial outcome of the suppression of C5a‐C5aR1 signaling, further supporting its potential as an effective targeted therapy to treat AD.Highlights
C5aR1 ablation restores long‐term potentiation in the Arctic model of AD.
C5aR1 ablation rescues region specific excessive pre‐synaptic loss.
C5aR1 antagonist, PMX205, rescues VGlut1 loss in the Tg2576 model of AD.
C1q tagging is not sufficient to induce VGlut1 microglial ingestion.
Astrocytes contribute to excessive pre‐synaptic loss at late stages of the disease.
Enantioselective Self‐Assembly of a Homochiral Tetrahedral Cage Comprising Only Achiral Precursors
Authors: Chen Yixin , Cao Ze , Feng Tinglong , Zhang Xiaobo , Li Zhaoyong , Dong Xue , Huang Shaoying , Liu Yingchun , Cao Xiaoyu , Sue Andrew C.‐H. , Peng Chuanhui , Lin Xufeng , Wang Linjun , Li Hao 
Published: January 2024

AbstractHow Nature synthesizes enantiomerically pure substances from achiral or racemic resources remains a mystery. In this study, we aimed to emulate this natural phenomenon by constructing chiral tetrahedral cages through self‐assembly, achieved by condensing two achiral compounds–a trisamine and a trisaldehyde. The occurrence of intercomponent CH⋅⋅⋅π interactions among the phenyl building blocks within the cage frameworks results in twisted conformations, imparting planar chirality to the tetrahedrons. In instances where the trisaldehyde precursor features electron‐withdrawing ester side chains, we observed that the intermolecular CH⋅⋅⋅π forces are strong enough to prevent racemization. To attain enantioselective self‐assembly, a chiral amine was introduced during the imine formation process. The addition of three equivalents of chiral amino mediator to one equivalent of the achiral trisaldehyde precursor formed a trisimino intermediate. This chiral compound was subsequently combined with the achiral trisamino precursor, leading to an imine exchange reaction that releasing the chiral amino mediator and formation of the tetrahedral cage with an enantiomeric excess (ee) of up to 75 %, exclusively composed of achiral building blocks. This experimental observation aligns with theoretical calculations based on the free energies of related cage structures. Moreover, since the chiral amine was not consumed during the imine exchange cycle, it enabled the enantioselective self‐assembly of the tetrahedral cage for multiple cycles when new batches of the achiral trisaldehyde and trisamino precursors were successively added.
Enantioselective Intramolecular ortho Photocycloaddition Reactions of 2‐Acetonaphthones
Authors: Yan Peng , Stegbauer Simone , Wu Qinqin , Kolodzeiski Elena , Stein Christopher J. , Lu Ping , Bach Thorsten 
Published: January 2024

Abstract2‐Acetonaphthones, which bear an alkenyl group tethered to its C1 carbon atom via an oxygen atom, were found to undergo an enantioselective intramolecular ortho photocycloaddition reaction. A chiral oxazaborolidine Lewis acid leads to a bathochromic absorption shift of the substrate and enables an efficient enantioface differentiation. Visible light irradiation (λ=450 nm) triggers the reaction which is tolerant of various groups at almost any position except carbon atom C8 (16 examples, 53–99 % yield, 80–97 % ee). Consecutive reactions were explored including a sensitized rearrangement to tetrahydrobiphenylenes, which occurred with full retention of configuration. Evidence was collected that the catalytic photocycloaddition occurs via triplet intermediates, and the binding mode of the acetonaphthone to the chiral Lewis acid was elucidated by DFT calculations.
A Potentiometric Dual‐Channel Microsensor Reveals that Fluctuation of H2S is Less pH‐Dependent During Spreading Depolarization in the Rat Brain
Authors: Liu Rantong , Zhang Shuai , Zeng Hui , Gao Nan , Yin Yongyue , Zhang Meining , Mao Lanqun 
Published: January 2024

AbstractSpreading depolarization (SD) is one of the most common neuropathologic phenomena in the nervous system, relating to numerous diseases. However, real‐time monitoring the rapid chemical changes during SD to probe the molecular mechanism remains a great challenge. We develop a potentiometric dual‐channel microsensor for simultaneous monitoring of H2S and pH featuring excellent selectivity and spatiotemporal resolution. Using this microsensor we first observe real time changes of H2S and pH in the rat brain induced by SD. This changes of H2S are completely suppressed when the rat pre‐treats with aminooxyacetic acid (AOAA), a blocker to inhibit the H2S‐producing enzyme, indicating H2S fluctuation might be related to enzyme‐dependent pathway during SD and less pH‐dependent. This study provides a new perspective for studying the function of H2S and the molecular basis of SD‐associated diseases.
Innovative and Effective Education Strategies for Adult Learners in the Perioperative Setting
Authors: Ward Laura , Gordon Abby , Kirkman Allyson 
Published: January 2024

ABSTRACTOngoing nursing education is vital for keeping nurses’ knowledge and skills current and promoting positive patient outcomes. Providing meaningful, quality nursing education in the perioperative setting requires the development and implementation of innovative and effective teaching strategies. Adult learning theory is complex, and it can be challenging to engage multigenerational perioperative staff members in education—often, a variety of creative teaching modalities are required to bridge the gaps among learning styles. This article reviews the use of experiential learning, various kinesthetic activities, advanced technology, microlearning, and other methods that may be helpful to overcome the challenges of providing education to adult learners in the perioperative setting. Educators should promote critical thinking and student engagement to encourage adult learners to be active participants in their continuing education.
Clinical Issues — February 2024
Authors: Cahn Julie 
Published: January 2024

THIS MONTH
Retaining count sheets in the patient's medical record
Key words: count sheet, medical record, procedural risk factor, retained surgical item, surgical count.
Counting instruments during cavity closure
Key words: uterine cavity, abdominal cavity, fetal procedure, cesarean delivery, instrument count.
Surgical wound classification of procedures involving cancer removal
Key words: cancer, contamination, infection, surgical wound classification, decision making.
Placing an electrosurgical unit dispersive electrode over a tattoo
Key words: dispersive electrode placement, metallic pigment, burn, tattoo, electrosurgical unit (ESU).
Hydroxychloroquine in Lupus Pregnancy and Risk of Preeclampsia
Authors: Rector Amadeia , Marić Ivana , Chaichian Yashaar , Chakravarty Eliza , Cantu Miranda , Weisman Michael H. , Shaw Gary M. , Druzin Maurice L. , Simard Julia F. 
Published: January 2024

ObjectiveSystemic lupus erythematosus (SLE) disproportionately affects women during childbearing years, and hydroxychloroquine (HCQ) is the standard first‐line treatment. Preeclampsia complicates up to one‐third of pregnancies in lupus patients, although reports vary by parity and multifetal gestation. We investigated whether taking HCQ early in pregnancy may reduce the risk of preeclampsia.MethodsWe studied 1,068 live birth singleton pregnancies among 1,020 privately insured patients with SLE (2007–2016). HCQ treatment was defined as three months preconception through the first trimester, and prescription fills were a proxy for taking HCQ. Modified Poisson regression estimated risk ratios (RRs) and 95% confidence intervals (CIs), stratified by parity. Propensity scores accounted for confounders, and stratified analyses examined effect modification.ResultsApproximately 15% of pregnant patients were diagnosed with preeclampsia. In 52% of pregnancies, patients had one or more HCQ fills. Pregnant patients exposed to HCQ had more comorbidities, SLE activity, and azathioprine treatment. We found no evidence of a statistical association between HCQ and preeclampsia among nulliparous (RR 1.26 [95% CI 0.82–1.93]) and multiparous pregnancies (RR 1.20 [95% CI 0.80–1.70]). Additional controls for confounding decreased the RRs toward the null (nulliparous pregnancy, propensity score–adjusted [PS‐adj] RR 1.09 [95% CI 0.68–1.76]; multiparous pregnancy, PS‐adj RR 1.01 [95% CI 0.66–1.53]).ConclusionUsing a large insurance‐based database, we did not observe a decreased risk of preeclampsia associated with HCQ treatment in pregnancy, although we cannot rule out residual and unmeasured confounding and misclassification. Further studies leveraging large population‐based data and prospective collection could characterize how HCQ influences preeclampsia risk in pregnant patients with SLE and among persons at greater risk of hypertensive disorders of pregnancy.