bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lateral gating mechanism and plasticity of the β-barrel assembly machinery complex in micelles and Escherichia coli
Authors: Gopinath Aathira , Rath Tobias , Morgner Nina , Joseph Benesh 
Published: January 2024

Abstract
The β-barrel assembly machinery (BAM) mediates the folding and insertion of the majority of outer membrane proteins (OMPs) in gram-negative bacteria. BAM is a penta-heterooligomeric complex consisting of the central β-barrel BamA and four interacting lipoproteins BamB, C, D, and E. The conformational switching of BamA between inward-open (IO) and lateral-open (LO) conformations is required for substrate recognition and folding. However, the mechanism for the lateral gating or how the structural details observed in vitro correspond with the cellular environment remains elusive. In this study, we addressed these questions by characterizing the conformational heterogeneity of BamAB, BamACDE, and BamABCDE complexes in detergent micelles and/or Escherichia coli using pulsed dipolar electron spin resonance spectroscopy (PDS). We show that the binding of BamB does not induce any visible changes in BamA, and the BamAB complex exists in the IO conformation. The BamCDE complex induces an IO to LO transition through a coordinated movement along the BamA barrel. However, the extracellular loop 6 (L6) is unaffected by the presence of lipoproteins and exhibits large segmental dynamics extending to the exit pore. PDS experiments with the BamABCDE complex in intact E. coli confirmed the dynamic behavior of both the lateral gate and the L6 in the native environment. Our results demonstrate that the BamCDE complex plays a key role in the function by regulating lateral gating in BamA.
Using Social Media to Identify the Effects of Congressional Viewpoints on Asset Prices
Authors: Bianchi Francesco , Gómez-Cram Roberto , Kung Howard 
Published: January 2024

Abstract
We use a high-frequency identification approach to document that individual politicians affect asset prices. We exploit the regular flow of viewpoints contained in Congress members’ tweets. Supportive (critical) tweets increase (decrease) the stock prices of the targeted firm and the corresponding industry in minutes around the tweet. The bulk of the stock price effects is concentrated in the tweets revealing news about future legislative action. The effects are amplified around committee meeting days, especially when the tweet originates from committee members and influential politicians. Overall, we show that Congress members’ social media accounts are an important source of political news.
Shorter total sleep time is associated with lower CD4+/CD8+ T cell ratios in virally suppressed men with HIV
Authors: Borker Priya V , Macatangay Bernard J , Margolick Joseph B , Punjabi Naresh M , Rinaldo Charles R , Stosor Valentina , Hyong-Jin Cho Joshua , McKay Heather , Patel Sanjay R 
Published: January 2024

AbstractStudy ObjectivesAlthough poor sleep quality is associated with lower CD4+ T cell counts among people living with HIV (PLWH), the association between objective sleep metrics and T lymphocyte subset counts is unknown. We evaluated the association between polysomnography (PSG) derived sleep metrics and T lymphocyte subpopulations in a cohort of men living with HIV.MethodsVirally suppressed men living with HIV participating in the Multicenter AIDS Cohort Study underwent home overnight PSG. We assessed the association of PSG parameters with CD4+ and CD8+ T cell counts and the CD4+/CD8+ T cell ratio.ResultsOverall, 289 men with mean (±SD) age 55.3 ± 11.3 years and mean CD4+ T cell count 730 ± 308 cells/mm3 were evaluated. Total sleep time (TST) was significantly associated with CD8+ but not CD4+ T cell counts. After adjusting for age, race, depressive symptoms, antidepressant use, and non-nucleoside reverse transcriptase inhibitors use, every hour of shorter TST was associated with an additional 33 circulating CD8+ T cells/mm3 (p = 0.05) and a 5.6% (p = 0.0007) decline in CD4+/CD8+ T cell ratio. In adjusted models, every hour of shorter rapid eye movement (REM) sleep was associated with an additional 113 CD8+ T cells/mm3 (p = 0.02) and a 15.1% lower CD4+/CD8+ T cell ratio (p = 0.006). In contrast, measures of sleep efficiency and sleep-disordered breathing were not associated with differences in T lymphocyte subpopulations.ConclusionsOur findings suggest that shorter TST and REM sleep durations are associated with differences in T lymphocyte subpopulations among men living with HIV. Addressing sleep may reflect a novel opportunity to improve immune function in PLWH.
Evaluating the National Action Plan (NAP) on antimicrobial resistance, and recommendations for the next 5-year NAP: a roundtable discussion
Authors: Neale Daisy , Cullen Lucky 
Published: January 2024

Abstract
The UK’s current 5-year National Action Plan (NAP) on tackling antimicrobial resistance (AMR), which runs from 2019 to 2024, is coming to an end. As such, the next 5-year NAP is currently under development, and progress made towards the UK’s commitments on AMR is being analysed. This analysis has involved the publication of an addendum to the current NAP, which includes some new and revised commitments. Applied Microbiology International (AMI) held a roundtable discussion in November 2022, inviting UK experts from across the human and animal sectors to discuss their thoughts on progress to date, as well as any recommendations and key considerations they thought should be taken forward. Overall, it was agreed that there is a major disparity between the increasing ambitions of the NAP, and the reducing financial support for achieving said ambitions. The gap between evidence generation and implementing recommendations into real-world settings also presents a key challenge to overcome, if the NAPs commitments are to be achieved. A lack of communication and funding appear to underpin many of the barriers that prevent the successful delivery of current NAP commitments and are therefore highlighted as key areas of concern for policymakers to consider when looking to tackle AMR. The focus should be on successfully implementing and delivering the current NAP commitments ahead of identifying future ambitions.
When two worlds collide: digital assets and your estate plan
Authors: Meyer Christo 
Published: January 2024

Abstract
With the digital world being forced to interact with the physical world when it comes to death and the transfer/succession of asset ownership, we currently have two very different worlds colliding. Even in our technology-focused world, people’s estate plans still only account for “traditional” assets, like a car, bank account, house, etc., whereas more and more individuals own digital assets. Planning for digital assets is much more involved than one may think; different laws and classifications of digital assets make planning for these types of assets very specialized, as you are working with a real risk that a digital asset may be lost forever.
Anticancer activity of zinc oxide nanoparticles on prostate and colon cancer cell line
Authors: Motafeghi Farzaneh , Mortazavi Parham , Shokrzadeh Mohammad 
Published: January 2024

Abstract

Introduction
Considering the numerous drug resistance in cancer and the advancement of science in nanomedicines, it was decided to compare the effectiveness of zinc oxide nanoparticles in colon and prostate cell lines. Considering the importance of factors and Oxidative stress pathways in cancer prevention, the aim of the study is based on oxidative stress mechanisms.

Methodes
In order to evaluate the effects of zinc oxide nanoparticles on colon and prostate cell lines, oxidative stress factors ROS, MDA, and GSH and mitochondrial function were evaluated. The data was analyzed with Prism v8 software, and the significance level was considered to be P &lt; 0.05.

Results
The results showed that nanoparticles induce ROS and reduce intracellular glutathione by destroying and disrupting mitochondrial function, and by increasing ROS production, damage to the lipid membrane and an increase in MDA were also evident. This effect was dose-dependent and the greatest at a concentration of 25 μg/mL. Also, ZnO nanoparticles performed better in the HT29 cell line than in the PC3 cell line.

Conclusion
This study showed that exposure of HT29 and PC3 cancer cells to zinc oxide nanoparticles at different concentrations inhibited growth by cytotoxic effects.
Mitochondrial impairment related to the immunotoxicity of the herbicides clomazone, glyphosate and sulfentrazone in THP-1 cells
Authors: Cestonaro Larissa Vivan , da Silva Artur Christian Garcia , Garcia Solange Cristina , Valadares Marize Campos , Arbo Marcelo Dutra 
Published: January 2024

AbstractBackgroundPesticides are indispensable for the cultivation of crops, especially those of economic importance, such as soybeans. Data on the annual use of herbicides in crops show that they correspond to 50%, making it the most used in agriculture.AimTherefore, the aim of this study was to evaluate the toxicity of the three commercial herbicides (clomazone, glyphosate, and sulfentrazone) in THP-1 cells.MethodsCells were incubated with 0–5,000 mg/L of the herbicides for 24 h at 37 °C for cytotoxicity evaluation. Additionally, a few toxicological pathways such as reactive species generation, mitochondrial impairment, and interleukin profile, which have been previously involved in the toxicity of pesticides, were also evaluated.ResultsA potential immunotoxic effect of the herbicides on THP-1 cells was observed, especially glyphosate, as it is a powerful agent of cellular immunotoxicity. It was also possible to verify an increase in oxidative stress and IL-8 levels and mitochondrial dysfunction.ConclusionAll herbicides showed cytotoxic effects in THP-1 monocytes, which were related to mitochondrial impairment.
NextGenerationEU: Hamiltonian moment or European New Deal?
Authors: Van Malleghem Pieter-Augustijn 
Published: January 2024

Abstract
During the coronavirus disease (COVID-19) pandemic, the European Union (EU) took the unprecedented step of borrowing 750 billion euros on capital markets, part of which was to be spent in the form of grants. As prior interpretations of the Union’s constitution tended to suggest that it had no power for deficit spending, NextGenerationEU (NGEU) arguably constitutes a major constitutional transformation for the Union. As so often in the literature on the EU, the comparison was made with the federal system of the USA: Commentators likened the Union’s constitutional transformation to the transformation engineered by Hamilton, the financial founding father of the USA, as well as Roosevelt’s New Deal. When the USA was teetering on the brink of bankruptcy, Hamilton transformed the landscape of public debt in the USA by proposing to restructure and assume the debt of the states on the balance sheet of the federal government. For the first time, the public debt of the federal government of the USA played a critical role in its economy. The Great Depression prompted another transformation for the finances of the USA. Roosevelt promised his voters to bring back economic growth. During Roosevelt’s tenure in office, the public borrowing of the USA went up significantly. This contribution examines the constitutional controversies provoked by Hamilton’s and Roosevelt’s transformative policies. It argues that, in spite of some similarities, the question of the constitutionality of deficit spending was controversial neither in Hamilton’s nor in Roosevelt’s day. More fundamentally, it argues that both Roosevelt and Hamilton had a transformative vision for the economy not shared by NGEU.
Whole-Genome Sequence of Colletotrichum brevisporum Causing Anthracnose on Papaya (Carica papaya)
Authors: Yang Min , Zhou Chenping , Kuang Ruibin , Wu Xiaming , Wei Yuerong 
Published: January 2024

Colletotrichum spp. are the causal agents of papaya anthracnose, which seriously affects the quality and economic value of papaya fruits. Among them, Colletotrichum brevisporum is a new and important causal pathogen of anthracnose in papaya. The infection mechanisms of C. brevisporum are still unclear, and the genome sequence of C. brevisporum has not been released. In order to systemically explore the interaction between papaya and C. brevisporum, we sequenced the whole genome of the C. brevisporum strain C1, which was isolated from an infected papaya fruit in Guangdong Province, China. The assembly consists of 18 scaffolds with a genome size of 62.66 Mb. Furthermore, we identified genes that may be associated with pathogenicity, such as carbohydrate-active enzymes, secreted proteins, and secondary metabolite gene clusters. This genome resource will provide a valuable resource for future studies on the pathogenesis of C. brevisporum and comparative genomic analyses of the Colletotrichum genus. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Targeting ENPP1 depletion may be a promising therapeutic strategy for treating oral squamous cell carcinoma via cytotoxic autophagy‐related apoptosis
Authors: Ma Chao , Zhao Jizhi , Zhou Lian , Jia Congwei , Shi Yanping , Li Xing , Jihu Kedi , Zhang Tao 
Published: January 2024

AbstractENPP1 depletion closely related with modulation immunotherapy of several types of cancer. However, the role of ENPP1 correlation with autophagy in oral squamous cell carcinoma (OSCC) pathogenesis remain unknown. In this study, effects of ENPP1 on OSCC cells in vitro were examined by cell proliferation assay, transwell chamber assay, flow cytometry analysis and shRNA technique. Cellular key proteins related to cell autophagy and apoptosis were evaluated by Western blot and immunofluorescent staining. Moreover, functions of ENPP1 on OSCC process were observed in nude mouse model. We reported that overexpression of ENPP1 promote the growth of OSCC cell xenografts in nude mouse model. In contrast, ENPP1 downregulation significantly inhibits OSCC cancer growth and induces apoptosis both in vitro and in vivo, which are preceded by cytotoxic autophagy. ENPP1downregulation induces a robust accumulation of autophagosomes, increases LC3B‐II and decreases SQSTM1/p62 in ENPP1‐shRNA‐treated cells and xenografts. Mechanistic studies show that ENPP1 downregulation increases PRKAA1 phosphorylation leading to ULK1 activation. AMPK‐inhibition abrogates ENPP1 downregulation‐induced ULK1‐activation, LC3B‐turnover and SQSTM1/p62‐degradation while AMPK‐activation potentiates it's effects. Collectively, these data uncover that ENPP1 downregulation induces autophagic cell death in OSCC cancer, which may provide a potential therapeutic target for the treatment of OSCC.
An update on managing patients with multiple sclerosis in primary care
Authors: Hardeman Paula 
Published: January 2024

ABSTRACT
Multiple sclerosis (MS) is an immune-mediated inflammatory condition of the central nervous system causing periods of recurring inflammation and ultimately progression of symptoms over time. MS is a common cause of disability in younger patients. Evidence-based treatment for patients with MS early in their disease course prevents relapses and delays progression. Early treatments for MS were classified as immune-modulating; newer developments that suppress the immune system are more effective in preventing future relapses and progression but carry risks. The increased use of immunosuppressant therapies for patients with MS makes it imperative for clinicians to understand potential risks, benefits, and serious adverse reactions related to these therapies.
A Genome-Wide Association Study of Serum Metabolite Profiles in Septic Shock Patients
Authors: Daubney Emily R. , D’Urso Shannon , Cuellar-Partida Gabriel , Rajbhandari Dorrilyn , Peach Elizabeth , de Guzman Erika , McArthur Colin , Rhodes Andrew , Meyer Jason , Finfer Simon , Myburgh John , Cohen Jeremy , Schirra Horst Joachim , Venkatesh Balasubramanian , Evans David M. 
Published: January 2024

OBJECTIVES:
We sought to assess whether genetic associations with metabolite concentrations in septic shock patients could be used to identify pathways of potential importance for understanding sepsis pathophysiology.

DESIGN:
Retrospective multicenter cohort studies of septic shock patients.

SETTING:
All participants who were admitted to 27 participating hospital sites in three countries (Australia, New Zealand, and the United Kingdom) were eligible for inclusion.

PATIENTS:
Adult, critically ill, mechanically ventilated patients with septic shock (n = 230) who were a subset of the Adjunctive Corticosteroid Treatment in Critically Ill Patients with Septic Shock trial (ClinicalTrials.gov number: NCT01448109).

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
A genome-wide association study was conducted for a range of serum metabolite levels for participants. Genome-wide significant associations (p ≤ 5 × 10–8) were found for the two major ketone bodies (3-hydroxybutyrate [rs2456680] and acetoacetate [rs2213037] and creatinine (rs6851961). One of these single-nucleotide polymorphisms (SNPs) (rs2213037) was located in the alcohol dehydrogenase cluster of genes, which code for enzymes related to the metabolism of acetoacetate and, therefore, presents a plausible association for this metabolite. None of the three SNPs showed strong associations with risk of sepsis, 28- or 90-day mortality, or Acute Physiology and Chronic Health Evaluation score (a measure of sepsis severity).

CONCLUSIONS:
We suggest that the genetic associations with metabolites may reflect a starvation response rather than processes involved in sepsis pathophysiology. However, our results require further investigation and replication in both healthy and diseased cohorts including those of different ancestry.
Core Descriptor Sets for Rectal Prolapse Outcomes Research Using a Modified Delphi Consensus
Authors: Gurland Brooke H. , Olson Craig H. , McCarthy Madison S ,   Pelvic Floor Disorders Consortium Work Group, Bordeianou Liliana G. 
Published: January 2024

BACKGROUND:
There is wide variation for prolapse care.

OBJECTIVE:
To determine core descriptor sets for rectal prolapse to enhance outcomes research.

DESIGN:
Descriptors for patients undergoing rectal prolapse surgery were generated through a systematic review and expert opinion. Stakeholders were recruited internationally via list-serve and social media. Experts were encouraged to consider the minimum descriptors that could be considered during clinical care, and descriptors were grouped into core descriptor sets. Consensus was defined as > 70% agreement.

SETTING:
A three-round Delphi process using a 9-point Likert scale based on expert results was distributed via survey. The final interactive meeting used a polling platform.

PARTICIPANTS:
The Pelvic Floor Disorders Consortium interdisciplinary group convened to advance the clinical care of pelvic floor disorders.

MAIN OUTCOME MEASURES:
To achieve expert consensus for core descriptor sets for rectal prolapse using a modified Delphi method.

RESULTS:
A total of 206 providers participated with survey response rates of 82% and 88% respectively. Responders were from North America 56%, Europe 29%, and Latin America, Asia, Australia, New Zealand, and Africa 15%. Ninety-one percent identified as colorectal surgeons and 80% reported more than 5 years of experience (35% > 15 years). Fifty-seven attendees participated in the final meeting and voted on core descriptor sets. Ninety-three percent agreed that descriptors such as age, body mass index, frailty, nutrition, and American Society of Anesthesiology Score correlated to physiologic status. One hundred percent agreed to include baseline bowel function. 100% reported willingness to complete a synoptic operative report. Follow-up intervals 1,3,5 year after surgery (76%) with a collection of recurrence, and functional outcomes at those time periods reached an agreement.

LIMITATIONS:
Individual bias, self-identification of experts, and paucity of the knowledge related to rectal prolapse.

CONCLUSIONS:
This represents the first steps toward international consensus to unify language and data collection processes for rectal prolapse. See Video Abstract.
A Comparison of Preoperative Telephone Call Versus Mailed Educational Materials on Patient-Reported Outcomes in Mohs Micrographic Surgery
Authors: Taylor Savannah L. , Quan Eugenie Y. , Lockhart Ellen Rachel S. , Prickett Kyle A. , Phillips Mariana A. 
Published: January 2024

BACKGROUND
Preoperative education has uncertain benefits on the preparedness and satisfaction of patients undergoing Mohs micrographic surgery (MMS).

OBJECTIVE
We sought to determine the effect of a preoperative telephone call on preparedness and satisfaction in patients undergoing same-day office consultation and MMS.

MATERIALS AND METHODS
All new patients (N = 208) scheduled for same-day office consultation and MMS were mailed a standardized preoperative packet. Approximately half of those patients were randomly selected to also receive a preoperative phone call. On the day of the surgery, patients completed an anonymous preoperative and postoperative survey assessing their preparedness and satisfaction with the preoperative education received.

RESULTS
There was no significant difference in patient preparedness between the letter only (LO) and phone call and letter study groups. There was a significant difference in preoperative satisfaction—a higher percentage of LO patients were “somewhat satisfied” or “not satisfied” with the preoperative education received (p = .013).

CONCLUSION
Preoperative phone consultation, in addition to mailed educational materials, did not have a statistically significant effect on patient preparedness in patients undergoing MMS; however, there was a trend toward increased satisfaction with the preoperative education provided in patients who received a preoperative phone call.
The Fragility of Landmark Randomized Controlled Trials in the Plastic Surgery Literature
Authors: Ormseth Benjamin H. , ElHawary Hassan , Janis Jeffrey E. 
Published: January 2024

Background:
Randomized controlled trials (RCTs) are integral to the progress of evidenced-based medicine and help guide changes in the standards of care. Although results are traditionally evaluated according to their corresponding P value, the universal utility of this statistical metric has been called into question. The fragility index (FI) has been developed as an adjunct method to provide additional statistical perspective. In this study, we aimed to determine the fragility of 25 highly cited RCTs in the plastic surgery literature.

Methods:
A PubMed search was used to identify the 25 highest cited RCTs with statistically significant dichotomous outcomes across 24 plastic surgery journals. Article characteristics were extracted, and the FI of each article was calculated. Additionally, Altmetric scores were determined for each study to determine article attention across internet platforms.

Results:
The median FI score across included studies was 4 (2–7.5, interquartile range). The two highest FI scores were 208 and 58, respectively. Four studies (16%) had scores of 0 or 1. Three studies (12%) had scores of 2. All other studies (72%) had FI scores of 3 or higher. The median Altmetric score was 0 (0–3).

Conclusion:
The FI can provide additional perspective on the robustness of study results, but like the P value, it should be interpreted in the greater context of other study elements.