bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of bridging RT in relapsed/refractory diffuse large B-cell lymphoma undergoing CAR-T therapy: a multicenter study
Authors: Mannina Daniele , Bramanti Stefania , Chiappella Annalisa , Casadei Beatrice , De Philippis Chiara , Giordano Laura , Navarria Pierina , Mancosu Pietro , Taurino Daniela , Scorsetti Marta , Carlo-Stella Carmelo , Zinzani Pier Luigi , Santoro Armando , Corradini Paolo 
Published: November 2023

Abstract

The optimization of bridging regimen before chimeric antigen receptor (CAR)-T cell therapy in diffuse large B-cell lymphoma (DLBCL) may impact CAR-T efficacy and outcome. This retrospective study evaluates CAR-T outcome after bridging with radiotherapy (RT) and other bridging strategies. Among 148 patients with relapsed/refractory DLBCL who underwent leukapheresis for CAR-T manufacturing, 31 received RT-bridging, 84 chemotherapy (CT), 33 no-bridging or steroid-only. CAR-T cell were infused in 96.8% of RT-group. 89.2% of CT-group and 78.8% of no-bridge group (p = 0.079). Response to bridging was generally poor, but patients receiving RT had a significant reduction in LDH levels between pre- and post-bridging (p = 0.05). The one-year PFS was 51.2% in the RT group, 28.2% in the CT group, and 47.6% in the no-bridge group (p = 0.044); 1-year OS was 86.7% in the RT group, 52.7% in the CT group and 69% in the no-bridge group (p = 0.025). We observed a higher incidence of ICANS in patients who received CT than in others (20.0% CT group, 3.3% RT group, 7.7% no-bridge group; p = 0.05).
In conclusion, RT-bridging is associated with lower drop-out rate and CAR-T toxicity, and it might be preferred to other bridging strategies for patients with localized disease or for those with one prevalent symptomatic site.
Optimizing Experiments on the Time Course of Mental Processes: A Novel Method and Application for Measuring Keyboard Switches Bounce-back Time
Authors: Li Jia Qi , Fang Yu , Deng Yi 
Published: November 2023

Abstract
Keyboards, extensively utilized as input devices in reaction time tests, have undergone substantial technological advancements over the past decades. From the Hall Effect Switch introduced in 1965 to the modern Magnet Switches, keyboards based on the Hall Effect have seen innovations not only in hardware architecture but also in key detection mechanisms, notably Rapid Trigger. This has further amplified their advantages in minimizing input latency. Our research introduces an innovative experimental methodology for assessing the rebound time of keyboard switches. Employing this straightforward yet effective approach, we measured and calculated the bottom-out rebound time for three types of keyboard switches. The findings indicate that magnetic switches with Rapid Trigger functionality excel over mechanical keyboards in key actuation, release, and controller latency. The difference in bottom-out rebound time between the two exceeds 20ms. Consequently, we recommend the use of such keyboards in experiments related to the time course of mental processes, as this can mitigate errors induced by input devices and enhance the accuracy of the results.
Research on the Evolution of the Death Psychology of Chinese People in the Context of Lifting COVID-19 Pandemic Restrictions: A hybrid Study of Weibo Big Data
Authors: Li Xiaowen , He Yuanqing 
Published: November 2023

Abstract
Amid the novel coronavirus threat, individuals are compelled to ponder over profound existential matters like life and death.This study employs a hybrid research methodology, merging grounded theory with big data mining techniques, to delve into the psychological adaptation mechanism when individuals confront death threats amidst epidemic deregulation.We fetched texts related to death psychology keywords from the Sina Weibo platform. Post data cleaning, the database incorporated 3868 Weibo texts.Grounded theory forms the basis for data coding and theory formulation. Subsequently, big data mining techniques, including topic mining and semantic network analysis, are employed to validate the formulated codes and theories.The findings demonstrate that within the "Emotion-Cognition-Behavior-Value" framework, the implications of death threats manifest in four aspects: death anxiety, death cognition, coping efficacy, and sense of meaning.As time progresses, the study of death psychology can be segmented into four distinct phases: the tranquil phase prior to lifting pandemic restrictions, the threat phase at lifting pandemic restrictions onset, the coping phase mid-lifting pandemic restrictions, and the reformative phase post-lifting pandemic restrictions.The calculated outcomes of topic mining and semantic network analysis corroborate the coding results and theories derived from the grounded theory. This reaffirms that data mining technology can be a potent tool for validating grounded theory.
Enhancing Elderly Care Services: A Comprehensive Internet+ Community Home Indicator System
Authors: 赵 瑞 , Huo Mingshu , Tan Mingyang , Wang Lei , Liu Qiaoli , Li Jiangxu , Li Hongyu , Wang Ru 
Published: November 2023

Abstract

Background
In the context of the smart city and aging trends, Internet + community home-based care offers a novel approach to elderly care in China. However, limited research has focused on developing a comprehensive indicator system to address the diverse needs of elderly individuals residing at home. Based on existence needs, relationship needs and growth needs abbreviated as "ERG" demand theory. In response, this study leverages the ERG demand theory as a functional framework to establish a demand-oriented Internet + community home-based elderly service indicator system.
Methods
In this study, we adopt the ERG demand theory as the conceptual framework and employ a combination of the Delphi method and Analytic Hierarchy Process (AHP), thereby integrating both qualitative and quantitative research techniques to develop and assign weightings to the demand-orientated indicator system for Internet-based community home healthcare services for the elderly. Our research methodology is based on comprehensive literature reviews and collaborative group meetings.
Results
The study developed a demand-orientated Internet + community home healthy elderly service indicator system, comprising three primary indicators, 10 secondary indicators, and 46 tertiary indicators. These top-level indicators, ranked in descending order of importance, are medical and nursing services (0.493), living care services (0.311), and psychosocial/spiritual support services (0.196).
Conclusion
The indicator system serves the purpose of comprehending the health status and care requirements of elderly individuals confined to their homes while offering personalized services. In addition to addressing the fundamental needs of homebound older people through individualized care and expert medical guidance, this program places a significant emphasis on the spiritual and psychological well-being of these individuals to ensure a holistic approach to their physical and mental needs. This research posits that the indicator system can aid community healthcare workers in discerning the demand distribution for elderly services and optimizing resource allocation. By continuously evaluating and monitoring the indicators, this approach facilitates the enhancement of service quality and the fine-tuning of strategies, ultimately delivering efficient, safe, and high-quality elderly care services.
DNA methylation participates in drought stress memory and response to drought in Medicago ruthenica
Authors: Zi Na , Guo Huiqin , Ren Weibo , Fry Ellen L. , Yuan Feng , Liu Yaling 
Published: November 2023

Abstract
Drought is a serious environmental problem, affecting plant growth and development and resulting in severe yield loss. Many plants exposed to drought stress can generate stress memory, which provides some advantages for resisting recurrent drought. DNA methylation is a mechanism of stress memory formation, and many plants can alter methylation levels to form stress memories; however, it remains unclear whether Medicago ruthenicaexhibits drought stress memory, as the molecular mechanisms underlying this process have not been described in this species. Here, we conducted methylome and transcriptome sequencing to identify gene methylation and expression changes in plants with a history of two drought stress exposures (D2). Methylation analysis showed that drought stress resulted in an approximately 4.41% decrease in M. ruthenica genome methylation levels. CG dinucleotide contexts had the highest methylation levels, followed by CHG contexts, with CHH contexts having the lowest levels. Analysis of associations between methylation and transcript levels showed that most DNA methylation was negatively correlated with gene expression, except for methylation within CHH in gene promoter regions. Genes were divided into four categories according to correlation between methylation and gene expression; demethylated genes expression upregulation accounted for the vast majority (n = 692 genes), and included genes encoding key factors for abscisic acid (ABA) and proline synthesis. Demethylation of the promoter and body regions of these two genes induced increased gene expression levels. In conclusion, DNA methylation may contribute to drought stress memory formation and maintenance in M. ruthenica by increasing transcription levels of genes key for ABA and proline biosynthesis.
PJA2 suppresses colorectal cancer progression by controlling HDAC2 degradation and stability.
Authors: Chen Zhihao , Yang Peng , Jin Chi , Wang Tuo , Wang Ye , Peng Chaofan , Sun Qingyang , Xu Hengjie , Nie Hongxu , Wang Xiaowei , Feng Yifei , Sun Yueming 
Published: November 2023

Abstract
Background
The Praja Ring Finger Ubiquitin Ligase 2 (PJA2), one of the RING ligase family, has been reported to be degrading differential substrates and regulating diverse diseases. However, the E3 ligase function of PJA2 played in cancer development and progression, especially in colorectal cancer, is still almost unknown.
Methods
The correlation between PJA2 and clinical characteristics was explored through the TCGA and GEO data sets. The qRT-PCR and Immunohistochemical assays were employed to assess the expression of PJA2 in CRC tissues and cell lines. The biological functions of PJA2 were verified by cell counting kit-8, colony formation, flow cytometry, cell-derived xenograft, AOM/DSS colorectal tumorigenesis model and other in vivo and in vitro experiments. The RNA-seq, mass spectrometry analysis, GST pull-down, Chromatin Immunoprecipitation and Immunofluorescence were utilized to disclose the underlying molecular mechanisms of PJA2 in CRC proliferation and apoptosis.
Results
Our research discovered that PJA2 was abnormally downregulated in CRC tissues and cell lines, and the lower expression of PJA2 was detected, the poorer prognosis was present. Functionally, further in vivo and in vitro experiments jointly uncovered that PJA2 acted as a tumor suppressor gene via inhibiting tumor proliferation and promoting cancer cell apoptosis in CRC progression. Regarding mechanism, PJA2 could recognize HDAC2 through the RBD domain and bind with the N-terminal of HDAC2 to ubiquitinate and degrade HDAC2 at K90 residue. PJA2-mediated ubiquitination and degradation of HDAC2 could counteract the transcriptional suppression of the IFIT family and PJA2, thus facilitating the transcription of the IFIT family to promote cancer cell apoptosis and inhibit tumor proliferation.
Conclusion
Our data showed that PJA2 interacted with HDAC2, promoted the poly-ubiquitination and degradation of HDAC2, abrogated the transcriptional suppression of the IFIT family and PJA2, constituted a positive feedback loop, and prevented tumor proliferation. Hence, PJA2 might be a potential therapeutic target for CRC, and interruption of this positive feedback loop would be a treatment strategy to slow or restrain the progression of CRC.
Curvularia lingshanensis sp. nov., a New Pathogen Causing stem and leaf rot on Curcuma kwangsiensis and Its Sensitivity to Five Fungicides
Authors: Wang Hanyi , Su Jiali , Liu Ze , Lin Siyu , Guo Jingyi , Gao Qi , Zhou Hao 
Published: November 2023

Abstract

Curcuma kwangsiensis S. G. Lee et C. F. Liang (Zingiberaceae) is a traditional Chinese medicinal plant that is cultivated in the South-West of China. In this study, five fungal strains were isolated from diseased C. kwangsiensis plants in Lingshan County, Guangxi Province. The phylogenetic analyses, based on a combined dataset of ITS, GAPDH, and EF-1α gene sequences, revealed that the strains from this study formed a distinct clade separate from other known Curvularia species. Additionally, these strains were closely related to C. tamilnaduensis, C. radici-foliigena, and C. radicicola. Based on morphological observations, the strains exhibit distinct variations in conidiophore length, conidiogenous cell characteristics, as well as conidial size and shape, distinguishing them from similar species. Consequently, these five strains have been classified as a novel species, C. lingshanensis. Pathogenicity test carried out on the plants confirmed that the new species was pathogenic to C. kwangsiensis. Five fungicides were tested for their toxicity to the pathogen using the mycelial growth rate method. Results indicated that among the studied fungicides, 70% mancozeb, 10% difeno-conazole and 75% trifloxystrobin·tebuconazole had the highest antifungal activity against C. lingshanensis with EC50 values of 1.5233 ± 0.18 mg/L, 0.1748 ± 0.03 mg/L, 0.2557 ± 0.06 mg/L, respectively. This study provides the first report of a severe stem and leaf rot disease on C. kwangsiensis caused by C. lingshanensis and important guidance for preventing and controlling this disease effectively.
The role of HIF-1α silencing in late pregnancy hypoxia-induced autism-like behavior in rat offspring
Authors: Tang Jinghua , Yang Ying , Qu Ping , Chen Jie , Li Tingyu , Dai Ying 
Published: November 2023

Abstract
Autism spectrum disorder (ASD) is a neurodevelopmental disorder that can be caused by a variety of factors. Our previous study indicated that hypoxia-inducible factor 1 alpha (HIF-1α) plays a role in hypoxia-caused autism-like behavior. In this study, we investigated the mechanism by which HIF-1α contributes to prenatal hypoxia-induced autism-like behavior in vivo to provide an experimental basis for the treatment of ASD. We established a prenatal hypoxia model of pregnant rats by placing 17-day pregnant rats into a self-made hypoxia chamber filled with a nitrogen containing 10%±0.5% oxygen. Within 24 h after birth, the lateral ventricles of the prenatal hypoxia offspring rats were injected with a recombinant adeno-associated virus designed to silence HIF-1α expression. The autistic behavior of offspring rats in the HIF-1α silenced group was significantly alleviated compared with that of the prenatal hypoxia group. With the silencing of HIF-1α, the activity of phosphatase and tensin homolog (PTEN) increased and the PI3K/AKT pathway was inhibited by negative feedback. The mRNA expression level of vascular endothelial growth factor (VEGF) was decreased in the Si-HIF-1α silenced group and N-methyl D-aspartate receptor subtype 2 (NR2A) expression was downregulated. Thus, our study indicates that HIF-1α plays a role in hypoxia-induced autism-like behavior, and its regulatory effect may be achieved by inhibiting the activity of PTEN, resulting in activation of the PI3K signaling pathway. Synaptic plasticity regulation may also be involved.
Multi-dimensional comparison of abdominal obesity indices and insulin resistance indicators for assessing NAFLD
Authors: Shi Jiejun , Chen Jianhua , Zhang Zeqing , Qian Guoqing 
Published: November 2023

Abstract

Background The prevalence of non-alcoholic fatty liver disease (NAFLD) keeps increasing annually all around the world. Non-invasive assessment tools for the risk and severity of the disease are still limited. Insulin resistance (IR) and abdominal obesity (ABO) are closely related to NAFLD. Methods A retrospective large-scale, population-based study was conducted based on the data from the 2017–2018 cycle of the National Health and Nutrition Examination Survey (NHANES). Three ABO indexes, namely lipid accumulation product (LAP), visceral obesity index (VAI), waist circumference-triglyceride index (WTI), and three IR indices, including triglyceride glucose index (TyG), homeostasis model assessment of insulin resistance (HOMA-IR) and metabolic score for insulin resistance (METS-IR), were analyzed and compared for their relationships with NAFLD based on weighted multivariable logistic regression, spearman correlation heatmap, smooth curve fittings. Area under the curve (AUC) of receiver-operating characteristic (ROC) curve was used to evaluate the diagnostic capability of these indices for NAFLD. Statistical differences among the AUCs were calculated via Delong test. Results In total, 3095 participants were included in our study among which 1368 adults were diagnosed with NAFLD. Overall, all the six indices presented positive association with NAFLD. There was a claw-shaped curve between HOMA-IR, VAI, LAP and NAFLD while a smooth semi-bell curve was observed in TyG, METS-IR and WTI. Among all the six indices, LAP and HOMA-IR had the best diagnostic capability for NAFLD (for LAP: AUC = 0.8, Youden index = 0.48 and for HOMA-IR: AUC = 0.798, Youden index = 0.472) while VAI (AUC = 0.728, Youden index = 0.361) showed the lowest predictive value. Correlation heat map displayed that all the six indices were positively correlated with liver function, hepatic steatosis and fibrosis severity. Notably, in NAFLD group, IR indicators presented stronger association with alanine aminotransferase (ALT) compared against ABO indexes. Conclusions Based on our study, all the six indices can screen NAFLD among which LAP and HOMA-IR may be optimal predictors. Compared against ABO indexes, IR indices may be more sensitive to identify acute hepatic injure in patients with NAFLD.
The incidence and Risk Factors of fetuses with mirror-image dextrocardia with solitus inversus
Authors: Xu Wenjiao , Wei Yajuan , Gao Xinru , Yang Xiaoxue , Wang Liping , Gao Wenlian , Zhu Xumin , Liu Baomin 
Published: November 2023

Abstract
Objective: This research seeks to ascertain the prevalence and determinants of mirror-image dextrocardia in fetuses
Study Design: With December of 2022 as the reference point , we compiled colleted data on pregnant women who carried fetuses with mirror-image dextrocardia in Xi'an, Shaanxi Province: September-October 2022, November 2022, and December 2022-January 2023 An online questionnaire was distributed to 209 pregnant across china who had contracted COVID-19. The case group comprised women whose final menstrual cycle occurred in November 2022 and who had a fetus with mirror-image dextrocardia. Women with a November 2022 final menstrual period and a fetus without this condition made up the control group. To identify the risk factors associated with fetal mirror-image dextrocardia, both univariate and multivariate logistic regression analyses were employed.
Results: A significant difference was noted in the gestational age at COVID-19 infection women with a September to October 2022 and December 2022 to January 2023 final menstrual period who did not bear a fetus with mirror-image dextrocardia, and those with a November 2022 final menstrual period whose fetus exhibited this condition. The univariate and multivariate analyses conducted on pregnant women with a final menstrual period in November 2022 who had contracted COVID-19 revealed significant differences in the presence and duration of fever between those bearing fetuses with mirror-image dextrocardia and those without (P = 0.000).
Conclusion: The findings suggest two critical factors to the increased prevalence of fetal mirror-image dextrocardia : 1) the infection timing which occurs between the 4th and 6th week of pregnancy; 2) the presence of fever and its prolonged duration.
Automatic Evaluation of EDM Electrode Wear via Integration of Image Segmentation and 3D Registration (IS-3DR)
Authors: Ma Fubin , Zhou Tianfeng , Cui Ying , Uddin Md Nasir , Yao Xiaoqiang , Zeng Jiyong , Guo Weijia , Wang Xibin 
Published: November 2023

Abstract
Electrode wear (EW) during the electrical discharge machining (EDM) process determines surface topography accuracy. In this paper, an automatic evaluation method is proposed by integrating image segmentation and three-dimensional (3D) registration (IS-3DR) to evaluate the EW more accurately. First, the original two-dimensional (2D) image and the 3D point cloud were derived from a laser microscope image, and the 2D worn area was determined by automatic recognition and segmentation. Then, 3D registration between the 3D point cloud and the 2D worn area was utilized to qualitatively define the 3D EW area. Finally, the 3D EW volume was obtained quantitatively through microelement calculation based on the 3D EW area and the shape fitting of the original electrode. The results showed that the intersection over union (IOU) of image segmentation was higher than 97.5%, compared with the manually labeled image segmentation method. The reliability of the proposed IS-3DR evaluation method was confirmed. Additionally, EDM experiments are carried out to verify the proposed IS-3DR evaluation method. The impact of various discharge parameters on EW was illustrated in detail.
Orthotropic transplantation of the bioengineered lung using a mouse-scale perfusion-based bioreactor and human primary endothelial cells
Authors: Suzuki Takaya , Tomiyama Fumiko , Watanabe Tatsuaki , Miyanaga Jun , Suzuki Anna , Murai Sho , Suzuki Yuyo , Niikawa Hiromichi , Oishi Hisashi , Notsuda Hirotsugu , Watanabe Yui , Hirama Takashi , Onodera Ken , Togo Takeo , Noda Masafumi , Waddell Thomas , Karoubi Golnaz , Okada Yoshinori 
Published: November 2023

Abstract
Whole lung engineering and the transplantation of its products is an ambitious goal and ultimately a viable solution for alleviating the donor-shortage crisis for lung transplants. There are several limitations currently impeding progress in the field with a major obstacle being efficient revascularization of decellularized scaffolds, which requires an extremely large number of cells when using larger pre-clinical animal models. Here, we developed a simple but effective experimental pulmonary bioengineering platform by utilizing the lung as a scaffold. Revascularization of pulmonary vasculature using human umbilical cord vein endothelial cells was feasible using a novel in-house developed perfusion-based bioreactor. The endothelial lumens formed in the peripheral alveolar area were confirmed using a transmission electron microscope. The quality of engineered lung vasculature was evaluated using fractal dimension analysis of histological images. The engineered mouse lungs were successfully transplanted into the orthotopic thoracic cavity. The engineered vasculature in the lung scaffold showed blood perfusion after transplantation without significant hemorrhage. The mouse-based lung bioengineering system can be utilized as an efficient ex-vivo screening platform for lung tissue engineering.