Optics and quantitative assessment of corneal transparency
Authors: Plamann Karsten 
Published: January 2024

While some optical properties of cornea like its stromal birefringence had been known since the early nineteenth century, theories explaining its transparency remained inconclusive until the publication of David Maurice' emblematic paper in 1957 Maurice made a model calculation of light scattering by a single collagen fibril as a “dielectric needle”. The volume density of the fibrils being known, Maurice could demonstrate that in the absence of any order among the fibrils the cornea would be opaque. Maurice postulated the hexagonal structure which since has been confirmed by electron microscopy. In modern terms, the order among the collagen fibrils within a stromal collagen lamella is described by a “structure factor” in the radiative transfer equation. This term corresponds to a description of the structure in reciprocal space and is thus equivalent to the representation of crystalline lattices in the first Brillouin zone used for structural modelling in crystallography.In a recent study we further analysed the collagen structure of healthy and oedematous corneas on a nanometric level observed by transmission electron microscopy (TEM). By automated image analysis we could retrieve the so‐called “pair correlation function” and calculate light attenuation within the corresponding cornea. Our findings show that the 1/e light penetration depth within healthy cornea could be calculated as 7300 ± 800 μm, which explains well the transparency of healthy cornea. However, the photon mean free path of oedematous cornea turned out to be from two to three times that value. In oedematous cornea, the “dilution” effect of the scatters is in fact stronger than the degradation of transparency by the perturbation of the structure. The actual loss of transparency in oedematous cornea may therefore be attributed to scattering processes at the micrometric level induced by the augmented optical contrast at the sites of the keratocytes between the collagen lamellae.Perturbations in corneal transparency do not only hamper vision, they also impact optical imaging methods on cornea. This, in turn, permits to study the transparency properties of the corneal tissue. One of the main research interests of our group is to analyse corneal images recorded by optical coherence tomography (OCT) and to extract quantitative parameters like photon mean path length and the fraction of coherently transmitted light.Our partners could show that in the light scattering regime, a term in the radiation transfer equation may be neglected and the attenuation of the full field OCT signal in the volume of the tissue reduces to a Lambert–Beer law. We developed a data analysis algorithm based on Bayesian inference to extract the attenuation coefficient and used it for the analysis of ex vivo corneal high‐resolution FF‐OCT images.In a second step, we developed an image processing algorithm for clinical OCT images removing typical artefacts and compensating for inhomogeneous illumination and corneal curvature. We presently are able to extract corneal transparency parameters from routine clinical OCT images and to provide post‐op follow‐up after keratoplasty or other surgical interventions.